bluuebunny/crossref_metadata_2025 huggingface dataset:
Generation of Rapid and High-Quality Serum by Recombinant Prothrombin Activator Ecarin (RAPClot™)
Authors: Zhao Kong-Nan , Dimeski Goce , Masci Paul , Johnson Lambro , Wang Jingjing , de Jersey John , Grant Michael , Lavin Martin F.
Published: May 2024

We recently reported the potential application of recombinant prothrombin activator ecarin (RAPClot™) in blood diagnostics. In a new study, we describe RAPClot™ as an additive to develop a novel blood collection prototype tube that produces the highest quality serum for accurate biochemical analyte determination. The drying process of the RAPClot™ tube generated minimal effect on the enzymatic activity of the prothrombin activator. According to the bioassays of thrombin activity and plasma clotting, γ-radiation (>25 kGy) resulted in a 30–40% loss of the enzymatic activity of the RAPClot™ tubes. However, a visual blood clotting assay revealed that the γ-radiation-sterilized RAPClot™ tubes showed a high capacity for clotting high-dose heparinized blood (8 U/mL) within 5 min. This was confirmed using Thrombelastography (TEG), indicating full clotting efficiency under anticoagulant conditions. The storage of the RAPClot™ tubes at room temperature (RT) for greater than 12 months resulted in the retention of efficient and effective clotting activity for heparinized blood in 342 s. Furthermore, the enzymatic activity of the RAPClot™ tubes sterilized with an electron-beam (EB) was significantly greater than that with γ-radiation. The EB-sterilized RAPClot™ tubes stored at RT for 251 days retained over 70% enzyme activity and clotted the heparinized blood in 340 s after 682 days. Preliminary clinical studies revealed in the two trials that 5 common analytes (K, Glu, lactate dehydrogenase (LD), Fe, and Phos) or 33 analytes determined in the second study in the γ-sterilized RAPClot™ tubes were similar to those in commercial tubes. In conclusion, the findings indicate that the novel RAPClot™ blood collection prototype tube has a significant advantage over current serum or lithium heparin plasma tubes for routine use in measuring biochemical analytes, confirming a promising application of RAPClot™ in clinical medicine.
Geometry Optimization Algorithms in Conjunction with the Machine Learning Potential ANI-2x Facilitate the Structure-Based Virtual Screening and Binding Mode Prediction
Authors: Wang Luxuan , He Xibing , Ji Beihong , Han Fengyang , Niu Taoyu , Cai Lianjin , Zhai Jingchen , Hao Dongxiao , Wang Junmei
Published: May 2024

Structure-based virtual screening utilizes molecular docking to explore and analyze ligand–macromolecule interactions, crucial for identifying and developing potential drug candidates. Although there is availability of several widely used docking programs, the accurate prediction of binding affinity and binding mode still presents challenges. In this study, we introduced a novel protocol that combines our in-house geometry optimization algorithm, the conjugate gradient with backtracking line search (CG-BS), which is capable of restraining and constraining rotatable torsional angles and other geometric parameters with a highly accurate machine learning potential, ANI-2x, renowned for its precise molecular energy predictions reassembling the wB97X/6-31G(d) model. By integrating this protocol with binding pose prediction using the Glide, we conducted additional structural optimization and potential energy prediction on 11 small molecule–macromolecule and 12 peptide–macromolecule systems. We observed that ANI-2x/CG-BS greatly improved the docking power, not only optimizing binding poses more effectively, particularly when the RMSD of the predicted binding pose by Glide exceeded around 5 Å, but also achieving a 26% higher success rate in identifying those native-like binding poses at the top rank compared to Glide docking. As for the scoring and ranking powers, ANI-2x/CG-BS demonstrated an enhanced performance in predicting and ranking hundreds or thousands of ligands over Glide docking. For example, Pearson’s and Spearman’s correlation coefficients remarkedly increased from 0.24 and 0.14 with Glide docking to 0.85 and 0.69, respectively, with the addition of ANI-2x/CG-BS for optimizing and ranking small molecules binding to the bacterial ribosomal aminoacyl-tRNA receptor. These results suggest that ANI-2x/CG-BS holds considerable potential for being integrated into virtual screening pipelines due to its enhanced docking performance.
APOA1/C3/A4/A5 Gene Cluster at 11q23.3 and Lipid Metabolism Disorders: From Epigenetic Mechanisms to Clinical Practices
Authors: Xiao Qianqian , Wang Jing , Wang Luyun , Ding Hu
Published: May 2024

The APOA1/C3/A4/A5 cluster is an essential component in regulating lipoprotein metabolism and maintaining plasma lipid homeostasis. A genome-wide association analysis and Mendelian randomization have revealed potential associations between genetic variants within this cluster and lipid metabolism disorders, including hyperlipidemia and cardiovascular events. An enhanced understanding of the complexity of gene regulation has led to growing recognition regarding the role of epigenetic variation in modulating APOA1/C3/A4/A5 gene expression. Intensive research into the epigenetic regulatory patterns of the APOA1/C3/A4/A5 cluster will help increase our understanding of the pathogenesis of lipid metabolism disorders and facilitate the development of new therapeutic approaches. This review discusses the biology of how the APOA1/C3/A4/A5 cluster affects circulating lipoproteins and the current progress in the epigenetic regulation of the APOA1/C3/A4/A5 cluster.
Impact of TNFRSF1B (rs3397, rs1061624 and rs1061622) and IL6 (rs1800796, rs1800797 and rs1554606) Gene Polymorphisms on Inflammatory Response in Patients with End-Stage Kidney Disease Undergoing Dialysis
Authors: Coimbra Susana , Rocha Susana , Catarino Cristina , Valente Maria João , Rocha-Pereira Petronila , Sameiro-Faria Maria , Oliveira José Gerardo , Madureira José , Fernandes João Carlos , Miranda Vasco , Belo Luís , Bronze-da-Rocha Elsa , Santos-Silva Alice
Published: May 2024

We aimed to study the impact of polymorphisms in the genes encoding interleukin-6 (IL6) and tumor necrosis factor receptor-2 (TNFR2), reported to be mortality risk predictors, in patients with end-stage kidney disease (ESKD) undergoing dialysis. TNFRSF1B (rs3397, rs1061624, and rs1061622) and IL6 (rs1800796, rs1800797, and rs1554606) polymorphisms were studied in patients with ESKD and controls; the genotype and allele frequencies and the associations with inflammatory and erythropoiesis markers were determined; deaths were recorded throughout the following two years. The genotype and allele frequencies for the TNFRSF1B rs3397 polymorphism were different in these patients compared to those in the controls and the global and European populations, and patients with the C allele were less common. Patients with the CC genotype for TNFRSF1B rs3397 presented higher hemoglobin and erythrocyte counts and lower TNF-α levels, suggesting a more favorable inflammatory response that seems to be associated with erythropoiesis improvement. Patients with the GG genotype for TNFRSF1B rs1061622 showed lower serum ferritin levels. None of the TNFRSF1B (rs3397, rs1061624, and rs1061622) or IL6 (rs1800796, rs1800797, and rs1554606) polymorphisms had a significant impact on the all-cause mortality rate of Portuguese patients with ESKD.
The Spatiotemporal Expression of SOCS3 in the Brainstem and Spinal Cord of Amyotrophic Lateral Sclerosis Mice
Authors: Lin Ching-Yi , Vanoverbeke Veronica , Trent David , Willey Kathryn , Lee Yu-Shang
Published: May 2024

Amyotrophic lateral sclerosis (ALS) is characterized by the progressive loss of motor neurons from the brain and spinal cord. The excessive neuroinflammation is thought to be a common determinant of ALS. Suppressor of cytokine signaling-3 (SOCS3) is pathologically upregulated after injury/diseases to negatively regulate a broad range of cytokines/chemokines that mediate inflammation; however, the role that SOCS3 plays in ALS pathogenesis has not been explored. Here, we found that SOCS3 protein levels were significantly increased in the brainstem of the superoxide dismutase 1 (SOD1)-G93A ALS mice, which is negatively related to a progressive decline in motor function from the pre-symptomatic to the early symptomatic stage. Moreover, SOCS3 levels in both cervical and lumbar spinal cords of ALS mice were also significantly upregulated at the pre-symptomatic stage and became exacerbated at the early symptomatic stage. Concomitantly, astrocytes and microglia/macrophages were progressively increased and reactivated over time. In contrast, neurons were simultaneously lost in the brainstem and spinal cord examined over the course of disease progression. Collectively, SOCS3 was first found to be upregulated during ALS progression to directly relate to both increased astrogliosis and increased neuronal loss, indicating that SOCS3 could be explored to be as a potential therapeutic target of ALS.
Theoretical and Experimental Research on Deflection of Hollow Slabs Set by Thin-Walled Corrugated Tubes on Simply Supported and Fixed Constraints in Opposite Sides
Authors: Yang Feng , Liu Jun , Liu Yongbing , Hou Qingming
Published: May 2024

Hollow floors are widely used in structures with a large span that bear a large load. In this study, we propose a hollow floor with built-in corrugated pipes as a filling material that has the advantages of a lighter weight, higher stiffness, and lower cost than traditional floors. We first propose a novel form of stiffness by coupling the anisotropies of the material and the structure. This concept is then used to develop a theoretical formula to compute the deflection of a hollow slab set using corrugated pipes on simply supported and fixed constraints on opposite sides. We then use static loading tests to show that this hollow slab has excellent ductility and load-bearing capacity. Following this, we design a mixed finite element model of the hollow slab to predict its deflection by considering concrete, steel, and corrugated tubes. We then use six reference points on the hollow slab to verify the model in comparison with the results of the static loading test and the theoretical formula. The results show that while the maximum deformation occurred at point a1 in the middle of the slab, the maximum errors among the results of the theoretical formula, static loading tests, and the finite element model occurred at point a2. The maximum and minimum errors between the results of the theoretical prediction and the outcomes of the static loading test were 9.09% and 0%, while those between the results of the theoretical prediction and the finite element model were 8.92% and 1.19%, respectively. The proposed hollow slab, set using thin-walled corrugated tubes, can be used in a variety of engineering designs.
A Method for Measuring the Visual Coherence of Buildings in Residential Historic Areas: A Case Study of the Xiaoxihu Historic Area in Nanjing, China
Authors: Xu Yipin , Pan Zejia
Published: May 2024

Residential historic areas are currently the main focus of urban renewal efforts in China, primarily consisting of traditional residential buildings with similar characteristics. Hence, the visual coherence of buildings (VCoB) plays a crucial role in such areas, not only pertaining to the visual quality of the urban landscape but also regarding the preservation of historic features. The accurate measurement of the VCoB is a prerequisite for undertaking optimization efforts. However, discussions of the VCoB in the built environment are limited and seldom address residential historic areas, and methods for measuring the VCoB have yet to be refined. Therefore, taking the Xiaoxihu Historic Area in Nanjing as a case study, this work aimed to develop a more refined method for measuring the VCoB in residential historic areas based on objective physical features. The method is mainly based on the human-level perspective, identifying three key visual elements within this perspective as the objects of measurement. It collected visual data through photography, with deep learning and computer graphics processing tools being utilized to identify and extract the visual elements. Then, the study established a corresponding framework of indicators for different visual elements and optimizes the methods for indicator calculation. Through an assessment involving professionals, we validated the high accuracy of the measurement method proposed in this study. Furthermore, the study discusses factors affecting the VCoB, methods to enhance the VCoB, and the required degree of the VCoB based on the results of the measurement. The method developed in this research will provide support for the visual analysis of the urban built environment and urban renewal practices.
Quantitative Study on the Impact of Surcharge on Nearby Foundations
Authors: Li Wu , Zhang Jinzhang , Chen Hui , Ni Jiaze , Zhang Dongming
Published: May 2024

Situated within the context of a soft ground foundation at an iron ore mining site, this study investigates the impact of substantial surcharges on the settlement of such foundations and the adjacent infrastructure. By employing the finite-difference numerical software FLAC3D 6.0, a series of three-dimensional simulations were conducted to assess the stress response and deformation of gallery pile foundations, shallow foundations, and mine shed pile foundations to step loading. This study integrates the analysis of soil strength augmentation under considerable stress and its attenuation characteristics under significant deformation. Various reinforcement measures, such as the implementation of stone columns, prefabricated vertical drain, and surcharge preloading techniques, were examined for their capacity to consolidate the foundation, reduce settlement, and mitigate impacts on adjacent structures. The results reveal that horizontal displacements in the pile and shallow foundations escalate progressively with additional surcharge throughout the operational period. The most pronounced horizontal deviation in the pile foundations is observed at the juncture between sand and silt strata. Stone columns act effectively as a barrier to the sliding surface, consequently reducing the influence of surcharge on the movement of the foundation.
Preoperative Bone Loss Predicts Decreased Survival Associated with Microvascular Invasion after Resection of Hepatocellular Carcinoma
Authors: Ishida Takashi , Miki Atsushi , Sakuma Yasunaru , Watanabe Jun , Endo Kazuhiro , Sasanuma Hideki , Teratani Takumi , Kitayama Joji , Sata Naohiro
Published: May 2024

Background: Osteopenia is a well-known risk factor for survival in patients with hepatocellular carcinoma; however, it is unclear whether osteopenia can apply to both genders and how osteopenia is associated with cancer progression. The aim of this study was to elucidate whether osteopenia predicts reduced survival in regression models in both genders and whether osteopenia is associated with the pathological factors associated with reduced survival. Methods: This study included 188 consecutive patients who underwent hepatectomy. Bone mineral density was assessed using computed tomography (CT) scan images taken within 3 months before surgery. Non-contrast CT scan images at the level of the 11th thoracic vertebra were used. The cutoff value of osteopenia was calculated using a threshold value of 160 Hounsfield units. Overall survival (OS) curves and recurrence-free survival (RFS) were constructed using the Kaplan–Meier method, as was a log-rank test for survival. The hazard ratio and 95% confidence interval for overall survival were calculated using Cox’s proportional hazard model. Results: In the regression analysis, age predicted bone mineral density. The association in females was greater than that in males. The OS and RFS of osteopenia patients were shorter than those for non-osteopenia patients. According to univariate and multivariate analyses, osteopenia was an independent risk factor for OS and RFS. The sole pathological factor associated with osteopenia was microvascular portal vein invasion. Conclusion: Models suggest that osteopenia may predict decreased OS and RFS in patients undergoing resection of hepatocellular carcinoma due to the mechanisms mediated via microvascular portal vein invasion.
Lung Clearance Index as a Screening Parameter of Pulmonary Impairment in Patients under Immune Checkpoint Therapy: A Pilot Study
Authors: Steinbach Maya-Leonie C. , Eska Jakob , Weitzel Julia , Görges Alexandra R. , Tietze Julia K. , Ballmann Manfred
Published: May 2024

Background: Immune checkpoint blockade (ICB) has presented a breakthrough in the treatment of malignant tumors and increased the overall survival of patients with various tumor entities. ICB may also cause immune-related adverse events, such as pneumonitis or interstitial lung disease. The lung clearance index (LCI) is a multiple-breath washout technique offering information on lung pathology in addition to conventional spirometry. It measures the degree of pulmonary ventilation inhomogeneity and allows early detection of pulmonary damage, especially that to peripheral airways. Methods: This cross-sectional study compared the lung function of patients with melanoma or metastatic cutaneous squamous cell carcinoma who received programmed cell death 1 (PD-1) and cytotoxic T-Lymphocyte-associated Protein 4 (CTLA-4) antibodies, alone or in combination, to age- and sex-matched controls. Lung function was assessed using spirometry, according to American Thoracic Society and European Respiratory Society standards, the LCI, and a diffusion capacity of carbon monoxide (DLCO) measurement. Results: Sixty-one screened patients and thirty-eight screened controls led to nineteen successfully included pairs. The LCI in the ICB-treated patients was 8.41 ± 1.15 (mean ± SD), which was 0.32 higher compared to 8.07 ± 1.17 in the control group, but the difference was not significant (p = 0.452). The patients receiving their ICB therapy for under five months showed a significantly lower LCI (7.98 ± 0.77) compared to the ICB patients undergoing therapy for over five months (9.63 ± 1.22) at the point of testing (p = 0.014). Spirometric analysis revealed that the forced expiratory volume between 25 and 75% of the forced vital capacity (FEF25–75%) in the ICB-treated patients was significantly reduced (p = 0.047) compared to the control group. DLCO (%predicted and adjusted for hemoglobin) was 94.4 ± 19.7 in the ICB patients and 93.4 ± 21.7 in the control group (p = 0.734). Conclusions: The patients undergoing ICB therapy showed slightly impaired lung function compared to the controls. Longer periods of ICB treatment led to deterioration of the LCI, which may be a sign of a subclinical inflammatory process. The LCI is feasible and may be easily integrated into the clinical daily routine and could contribute to early detection of pulmonary (auto-)inflammation.
Triple Negative Breast Cancer: Molecular Subtype-Specific Immune Landscapes with Therapeutic Implications
Authors: Syrnioti Antonia , Petousis Stamatios , Newman Lisa A. , Margioula-Siarkou Chrysoula , Papamitsou Theodora , Dinas Konstantinos , Koletsa Triantafyllia
Published: May 2024

Triple Negative Breast Cancer (TNBC) is characterized by distinct molecular subtypes with unique biological and clinical features. This systematic review aimed to identify articles examining the differences in the tumor immune microenvironment (TIME) across different TNBC molecular subtypes. Six studies meeting inclusion criteria were analyzed, utilizing gene expression profiling and bioinformatic analyses to classify TNBC samples into molecular subtypes, as well as immunohistochemistry and cell deconvolution methods to characterize the TIME. Results revealed significant heterogeneity in immune cell composition among TNBC subtypes, with the immunomodulatory (IM) subtype demonstrating robust immune infiltration, composed mainly of adaptive immune cells along with an increased density of CTLA-4+ and PD-1+ TILs, high PD-L1 tumor cell expression, and upregulation of FOXP3+ Tregs. A more immunosuppressive TIME with a predominance of innate immune cells and lower levels of tumor-infiltrating lymphocytes (TILs) was observed in luminal androgen receptor (LAR) tumors. In mesenchymal stem-like (MSL) tumors, the TIME was mainly composed of innate immune cells, with a high number of M2 tumor-associated macrophages (TAMs), while the BL and M tumors displayed poor adaptive and innate immune responses, indicating an “immune-cold” phenotype. Differential activation of signaling pathways, genomic diversity, and metabolic reprogramming were identified as contributors to TIME heterogeneity. Understanding this interplay is crucial for tailoring therapeutic strategies, especially regarding immunotherapy.
Using Patient-Specific 3D Modeling and Simulations to Optimize Microwave Ablation Therapy for Liver Cancer
Authors: Heshmat Amirreza , O’Connor Caleb S. , Albuquerque Marques Silva Jessica , Paolucci Iwan , Jones Aaron Kyle , Odisio Bruno C. , Brock Kristy K.
Published: May 2024

Microwave ablation (MWA) of liver tumors presents challenges like under- and over-ablation, potentially leading to inadequate tumor destruction and damage to healthy tissue. This study aims to develop personalized three-dimensional (3D) models to simulate MWA for liver tumors, incorporating patient-specific characteristics. The primary objective is to validate the predicted ablation zones compared to clinical outcomes, offering insights into MWA before therapy to facilitate accurate treatment planning. Contrast-enhanced CT images from three patients were used to create 3D models. The simulations used coupled electromagnetic wave propagation and bioheat transfer to estimate the temperature distribution, predicting tumor destruction and ablation margins. The findings indicate that prolonged ablation does not significantly improve tumor destruction once an adequate margin is achieved, although it increases tissue damage. There was a substantial overlap between the clinical ablation zones and the predicted ablation zones. For patient 1, the Dice score was 0.73, indicating high accuracy, with a sensitivity of 0.72 and a specificity of 0.76. For patient 2, the Dice score was 0.86, with a sensitivity of 0.79 and a specificity of 0.96. For patient 3, the Dice score was 0.8, with a sensitivity of 0.85 and a specificity of 0.74. Patient-specific 3D models demonstrate potential in accurately predicting ablation zones and optimizing MWA treatment strategies.
Standardized Digital Image Analysis of PD-L1 Expression in Head and Neck Squamous Cell Carcinoma Reveals Intra- and Inter-Sample Heterogeneity with Therapeutic Implications
Authors: Deuss Eric , Kürten Cornelius , Fehr Lara , Kahl Laura , Zimmer Stefanie , Künzel Julian , Stauber Roland H. , Lang Stephan , Hussain Timon , Brandau Sven
Published: May 2024

For practical reasons, in many studies PD-L1 expression is measured by combined positive score (CPS) from a single tumor sample. This does not reflect the heterogeneity of PD-L1 expression in head and neck squamous cell carcinoma (HNSCC). We investigated the extent and relevance of PD-L1 expression heterogeneity in HNSCC analyzing primary tumors and recurrences (LRs), as well as metastases. Tumor tissue from 200 HNSCC patients was immunohistochemically stained for PD-L1 and analyzed using image-analysis software QuPath v3.4 with multiple specimens per patient. CPS was ≥20 in 25.6% of primary tumors. Intra-tumoral heterogeneity led to a therapeutically relevant underestimation of PD-L1 expression in 28.7% of patients, when only one specimen per patient was analyzed. Inter-tumoral differences in PD-L1 expression between primary tumors and lymph node metastasis (LNM) or LR occurred in 44.4% and 61.5% (CPS) and in 40.6% and 50% of cases (TPS). Overall survival was increased in patients with CPS ≥ 1 vs. CPS < 1 in primary tumors and LNM (hazard ratio: 0.46 and 0.35; p < 0.005); CPS in LR was not prognostic. Our analysis shows clinically relevant intra- and inter-sample heterogeneity of PD-L1 expression in HNSCC. To account for heterogeneity and improve patient selection for immunotherapy, multiple sample analyses should be performed, particularly in patients with CPS/TPS < 1.
MRI for Differentiation between HPV-Positive and HPV-Negative Oropharyngeal Squamous Cell Carcinoma: A Systematic Review
Authors: Chen Linda L. , Lauwers Iris , Verduijn Gerda , Philippens Marielle , Gahrmann Renske , Capala Marta E. , Petit Steven
Published: May 2024

Human papillomavirus (HPV) is an important risk factor for oropharyngeal squamous cell carcinoma (OPSCC). HPV-positive (HPV+) cases are associated with a different pathophysiology, microstructure, and prognosis compared to HPV-negative (HPV−) cases. This review aimed to investigate the potential of magnetic resonance imaging (MRI) to discriminate between HPV+ and HPV− tumours and predict HPV status in OPSCC patients. A systematic literature search was performed on 15 December 2022 on EMBASE, MEDLINE ALL, Web of Science, and Cochrane according to PRISMA guidelines. Twenty-eight studies (n = 2634 patients) were included. Five, nineteen, and seven studies investigated structural MRI (e.g., T1, T2-weighted), diffusion-weighted MRI, and other sequences, respectively. Three out of four studies found that HPV+ tumours were significantly smaller in size, and their lymph node metastases were more cystic in structure than HPV− ones. Eleven out of thirteen studies found that the mean apparent diffusion coefficient was significantly higher in HPV− than HPV+ primary tumours. Other sequences need further investigation. Fourteen studies used MRI to predict HPV status using clinical, radiological, and radiomics features. The reported areas under the curve (AUC) values ranged between 0.697 and 0.944. MRI can potentially be used to find differences between HPV+ and HPV− OPSCC patients and predict HPV status with reasonable accuracy. Larger studies with external model validation using independent datasets are needed before clinical implementation.
High-Risk Genotypes of Human Papillomavirus at Diverse Anogenital Sites among Chinese Women: Infection Features and Potential Correlation with Cervical Intraepithelial Neoplasia
Authors: Zhao Chao , An Jiahui , Li Mingzhu , Li Jingran , Zhao Yun , Wang Jianliu , Xie Heidi Qunhui , Wei Lihui
Published: May 2024

Background: Both cervical cancer and cervical intraepithelial neoplasia (CIN) are associated with human papillomavirus (HPV) infection at different anogenital sites, but the infection features of high-risk (HR) HPVs at these sites and their association with cervical lesions have not been well characterized. Given the limitation of cervical HPV 16/18 test in screening patients with high-grade CIN (CIN 2+), studies on whether non-16/18 HR-HPV subtype(s) have potential as additional indicator(s) to improve CIN 2+ screening are needed. Methods: The infection of 15 HR-HPVs in vulva, anus, vagina, and cervix of 499 Chinese women was analyzed, and CIN lesion-associated HR-HPV subtypes were revealed. Results: In addition to the well-known cervical-cancer-associated HPV 16, 52, and 58, HPV 51, 53, and 56 were also identified as high-frequency detected subtypes prevalently and consistently present at the anogenital sites studied, preferentially in multi-infection patterns. HPV 16, 52, 58, 56, and 53 were the top five prevalent subtypes in patients with CIN 2+. In addition, we found that cervical HPV 33/35/52/53/56/58 co-testing with HPV 16/18 might improve CIN 2+ screening performance. Conclusion: This study provided a new insight into HR-HPV screening strategy based on different subtype combinations, which might be used in risk stratification clinically.
Regulating the Monomer Symmetry of Poly-Perylene-Diimides for Photocatalytic H2O2 Production
Authors: Zhou Meiwanqin , Yan Yukun , Zhang Jinsong , Xiao Jun
Published: May 2024

Photocatalysis technology is an economical and effective new energy technology which depends on the conversion and storage of light energy through an energy transfer process or charge transfer process. Recently, organic semiconductor photocatalytic materials with the advantages of controllable structure, broad spectral response, designability, and flexibility have received wide attention. In particular, the organic polymeric materials containing poly-perylene diimides (PDI) show significant promise in the realm of photocatalysis due to their impressive catalytic capabilities and wide spectral reactivity. However, a poor charge separation and transportation (CST) process undermines their photocatalytic efficiency in most polymer photocatalysts, as well as in PDI photocatalysts. In this context, we propose a new strategy through regulating the monomer symmetry to construct highly efficient PDI photocatalysts. As proof-of-concept, a series of new PDI-based organic supramolecular photocatalytic materials with full visible spectral response from the perspectives of both the π-π conjugated structure and the symmetry of chain structure are successfully synthesized. Meanwhile, the structural compositions, morphology features, electrical properties, and photocatalytic performances of those obtained PDI photocatalysts were systematically studied. The results shown that the as-prepared PDI-1,5NDA exhibits 1.6-fold and 3.7-fold higher levels of photosynthesis of H2O2 activity than those of PDI-1,4NDA and PDI-PDA, respectively, which could be ascribe to its lower symmetry and large π-conjugate systems greatly enhances the separation of charge carriers.
Endoscopic Ultrasound-Guided Tissue Sampling for the Cytohistological Diagnosis of Focal Liver Lesions
Authors: Lariño-Noia Jose , Jardi-Cuadrado Andrea , Dominguez-Muñoz Juan Enrique , Domínguez-Novoa Yessica , Galego Marco , Rama Alberto , de la Iglesia-Garcia Daniel , Martinez-Seara Xurxo , Abdulkader-Nallib Ihab , Iglesias-Garcia Julio
Published: May 2024

Background: Focal liver lesions (FLL) often require cytohistological evaluation. Endoscopic Ultrasound (EUS)-guided tissue acquisition (EUS-TA) is highly accurate in diagnosing pancreatic and gastrointestinal malignancies. The aim of our study was to evaluate the role of EUS-TA in the characterization of FLL. Methods: A retrospective analysis of a prospective database of patients who underwent EUS-TA for the evaluation of FLL. Diagnostic yield, adverse events and factors associated with diagnostic yield were evaluated as endpoints. The effect of variables such as needle size, lesion size, rapid on-site evaluation (ROSE) and the use of cytological or histological needles were analyzed. Results: A total of 114 cases were included (mean age 68.05 ± 11.35 years, 64 male). A correct diagnosis was made using EUS-TA in 100 of the 114 cases (diagnostic yield of 88%). The EUS-TA of additional extrahepatic lesions during the same EUS procedure increased the diagnostic yield to 94%. No adverse events were reported. Multivariate analysis did not identify any factor influencing the diagnostic yield. Conclusions: EUS-TA is a highly accurate and safe technique for the differential diagnosis of FLL and could be considered as the primary approach in this setting.
Development and Validation of an Ultrasonography-Based Machine Learning Model for Predicting Outcomes of Bruxism Treatments
Authors: Orhan Kaan , Yazici Gokhan , Önder Merve , Evli Cengiz , Volkan-Yazici Melek , Kolsuz Mehmet Eray , Bağış Nilsun , Kafa Nihan , Gönüldaş Fehmi
Published: May 2024

Background and Objectives: We aimed to develop a predictive model for the outcome of bruxism treatments using ultrasonography (USG)-based machine learning (ML) techniques. This study is a quantitative research study (predictive modeling study) in which different treatment methods applied to bruxism patients are evaluated through artificial intelligence. Materials and Methods: The study population comprised 102 participants with bruxism in three treatment groups: Manual therapy, Manual therapy and Kinesio Tape or Botulinum Toxin-A injection. USG imaging was performed on the masseter muscle to calculate muscle thickness, and pain thresholds were evaluated using an algometer. A radiomics platform was utilized to handle imaging and clinical data, as well as to perform a subsequent radiomics statistical analysis. Results: The area under the curve (AUC) values of all machine learning methods ranged from 0.772 to 0.986 for the training data and from 0.394 to 0.848 for the test data. The Support Vector Machine (SVM) led to excellent discrimination between bruxism and normal patients from USG images. Radiomics characteristics in pre-treatment ultrasound scans of patients, showing coarse and nonuniform muscles, were associated with a greater chance of less effective pain reduction outcomes. Conclusions: This study has introduced a machine learning model using SVM analysis on ultrasound (USG) images for bruxism patients, which can detect masseter muscle changes on USG. Support Vector Machine regression analysis showed the combined ML models can also predict the outcome of the pain reduction.
Ectopic “Ectopic” Gastric Mucosa
Authors: Haq Adeel , Haghighat Jahromi Amin
Published: May 2024

Meckel’s diverticulum is a developmental GI anomaly. It is a remnant of the omphalomesenteric duct (vitelline duct) and the most common congenital anomaly found in the small intestine. It contains ectopic/heterotopic gastric mucosa in half of the cases. Imaging investigations for diagnosing Meckel’s diverticulum may include a plain radiography; however, this has a very limited diagnostic value. A blind-ending fluid-filled structure can sometimes be seen with sonography, but again, this technique’s diagnostic value is limited due to multiple factors. A CT scan may be helpful in localizing the bleeding diverticulum, which can be better visualized with CT enterography. Diverticula containing gastric mucosa can be diagnosed with a higher sensitivity with Tc-99 scintigraphy. The typical location of Meckel’s diverticulum is within two feet of the ileocecal valve; thus, ectopic gastric mucosal uptake is typically seen in the lower right quadrant in scintigraphy. We present a rare case of Tc-99 pertechnetate scintigraphy showing ectopic gastric mucosa in the upper mid abdomen, which was surgically proven to be at the mid ileum. To our knowledge, there is no ectopic Meckel’s diverticulum case published in the literature. Familiarity with this atypical imaging presentation of relatively common ectopic gastric mucosa may help the radiologists in the timely diagnosis and management of the patient.
Human Papillomavirus Infection of the Oropharyngeal and Laryngeal Squamous Papilloma: Disparities in Prevalence and Characteristics
Authors: Kwak Jihye , Ahn Dongbin , Kim Mee-seon
Published: May 2024

Human papillomavirus (HPV) infection has emerged as an etiologic factor of squamous papilloma (SP). The oropharynx and larynx are common sites of SP, but studies on the prevalence of HPV infection in these sites are lacking. This study aimed to evaluate and compare the prevalence and characteristics of HPV infection in oropharyngeal SP (OPSP) and laryngeal SP (LSP). HPV detection and genotyping data of patients with pathologically confirmed OPSP and LSP were retrospectively analyzed. A total of 119 patients were enrolled, consisting of 93 patients with OPSP and 26 patients with LSP. Of those patients, 13 patients with OPSP and 14 patients with LSP were positive for HPV infection, accounting for a prevalence of 14.0% and 53.8%, respectively (p < 0.001). The most prevalent genotype was HPV16 in OPSP and HPV6 in LSP. Over two-thirds (69.2%) of HPV(+)-OPSP infections were high-risk types compared with 14.3% of HPV(+)-LSP infections (p = 0.004). The prevalence of HPV infection in patients with OPSP and LSP demonstrated no differences in terms of age, sex, and smoking status. These results could provide a better understanding of HPV infection in OPSP and LSP and serve as a background for the epidemiology of HPV-related tumorigenesis of the oropharynx and larynx.
Can Artificial Intelligence “Hold” a Dermoscope?—The Evaluation of an Artificial Intelligence Chatbot to Translate the Dermoscopic Language
Authors: Karampinis Emmanouil , Toli Olga , Georgopoulou Konstantina-Eirini , Kampra Elli , Spyridonidou Christina , Roussaki Schulze Angeliki-Victoria , Zafiriou Efterpi
Published: May 2024

This survey represents the first endeavor to assess the clarity of the dermoscopic language by a chatbot, unveiling insights into the interplay between dermatologists and AI systems within the complexity of the dermoscopic language. Given the complex, descriptive, and metaphorical aspects of the dermoscopic language, subjective interpretations often emerge. The survey evaluated the completeness and diagnostic efficacy of chatbot-generated reports, focusing on their role in facilitating accurate diagnoses and educational opportunities for novice dermatologists. A total of 30 participants were presented with hypothetical dermoscopic descriptions of skin lesions, including dermoscopic descriptions of skin cancers such as BCC, SCC, and melanoma, skin cancer mimickers such as actinic and seborrheic keratosis, dermatofibroma, and atypical nevus, and inflammatory dermatosis such as psoriasis and alopecia areata. Each description was accompanied by specific clinical information, and the participants were tasked with assessing the differential diagnosis list generated by the AI chatbot in its initial response. In each scenario, the chatbot generated an extensive list of potential differential diagnoses, exhibiting lower performance in cases of SCC and inflammatory dermatoses, albeit without statistical significance, suggesting that the participants were equally satisfied with the responses provided. Scores decreased notably when practical descriptions of dermoscopic signs were provided. Answers to BCC scenario scores in the diagnosis category (2.9 ± 0.4) were higher than those with SCC (2.6 ± 0.66, p = 0.005) and inflammatory dermatoses (2.6 ± 0.67, p = 0). Similarly, in the teaching tool usefulness category, BCC-based chatbot differential diagnosis received higher scores (2.9 ± 0.4) compared to SCC (2.6 ± 0.67, p = 0.001) and inflammatory dermatoses (2.4 ± 0.81, p = 0). The abovementioned results underscore dermatologists’ familiarity with BCC dermoscopic images while highlighting the challenges associated with interpreting rigorous dermoscopic images. Moreover, by incorporating patient characteristics such as age, phototype, or immune state, the differential diagnosis list in each case was customized to include lesion types appropriate for each category, illustrating the AI’s flexibility in evaluating diagnoses and highlighting its value as a resource for dermatologists.
Even and Odd Cat States of Two and Three Qubits in the Probability Representation of Quantum Mechanics
Authors: Mechler Matyas , Man’ko Margarita A. , Man’ko Vladimir I. , Adam Peter
Published: May 2024

We derive the probability representation of even and odd cat states of two and three qubits. These states are even and odd superpositions of spin-1/2 eigenstates corresponding to two opposite directions along the z axis. The probability representation of even and odd cat states of an oscillating spin-1/2 particle is also discussed. The exact formulas for entangled probability distributions describing density matrices of all these states are obtained.
Supervised-Learning-Based Method for Restoring Subsurface Shallow-Layer Q Factor Distribution
Authors: Zang Danfeng , Li Jian , Li Chuankun , Ma Mingxing , Guo Chenli , Wang Jiangang
Published: May 2024

The distribution of shallow subsurface quality factors (Q) is a crucial indicator for assessing the integrity of subsurface structures and serves as a primary parameter for evaluating the attenuation characteristics of seismic waves propagating through subsurface media. As the complexity of underground spaces increases, regions expand, and testing environments diversify, the survivability of test nodes is compromised, resulting in sparse effective seismic data with a low signal-to-noise ratio (SNR). Within the confined area defined by the source and sensor placement, only the Q factor along the wave propagation path can be estimated with relative accuracy. Estimating the Q factor in other parts of the area is challenging. Additionally, in recent years, deep neural networks have been employed to address the issue of missing values in seismic data; however, these methods typically require large datasets to train networks that can effectively fit the data, making them less applicable to our specific problem. In response to this challenge, we have developed a supervised learning method for the restoration of shallow subsurface Q factor distributions. The process begins with the construction of an incomplete labeled data volume, followed by the application of a block-based data augmentation technique to enrich the training samples and train the network. The uniformly partitioned initial data are then fed into the trained network to obtain output data, which are subsequently combined to form a complete Q factor data volume. We have validated this training approach using various networks, all yielding favorable results. Additionally, we compared our method with a data augmentation approach that involves creating random masks, demonstrating that our method reduces the mean absolute percentage error (MAPE) by 5%.
Fast Versatile Video Coding (VVC) Intra Coding for Power-Constrained Applications
Authors: Chen Lei , Cheng Baoping , Zhu Haotian , Qin Haowen , Deng Lihua , Luo Lei
Published: May 2024

Versatile Video Coding (VVC) achieves impressive coding gain improvement (about 40%+) over the preceding High-Efficiency Video Coding (HEVC) technology at the cost of extremely high computational complexity. Such an extremely high complexity increase is a great challenge for power-constrained applications, such as Internet of video things. In the case of intra coding, VVC utilizes the brute-force recursive search for both the partition structure of the coding unit (CU), which is based on the quadtree with nested multi-type tree (QTMT), and 67 intra prediction modes, compared to 35 in HEVC. As a result, we offer optimization strategies for CU partition decision and intra coding modes to lessen the computational overhead. Regarding the high complexity of the CU partition process, first, CUs are categorized as simple, fuzzy, and complex based on their texture characteristics. Then, we train two random forest classifiers to speed up the RDO-based brute-force recursive search process. One of the classifiers directly predicts the optimal partition modes for simple and complex CUs, while another classifier determines the early termination of the partition process for fuzzy CUs. Meanwhile, to reduce the complexity of intra mode prediction, a fast hierarchical intra mode search method is designed based on the texture features of CUs, including texture complexity, texture direction, and texture context information. Extensive experimental findings demonstrate that the proposed approach reduces complexity by up to 77% compared to the latest VVC reference software (VTM-23.1). Additionally, an average coding time saving of 70% is achieved with only a 1.65% increase in BDBR. Furthermore, when compared to state-of-the-art methods, the proposed method also achieves the largest time saving with comparable BDBR loss. These findings indicate that our method is superior to other up-to-date methods in terms of lowering VVC intra coding complexity, which provides an elective solution for power-constrained applications.
A Practical Superconducting DC Dynamo for Charging Conduction-Cooled HTS Magnet
Authors: Zhai Yujia , Mu Chunran , Wang Jinduo , Zhu Litong , Weng Tingkun , Li Zhuo , Wu Xingzheng , Shen Liufei , Liu Jianhua , Wang Qiuliang
Published: May 2024

At present, HTS magnets cannot operate in the real closed-loop persistent current mode due to the existence of joint resistance, flux creep, and AC loss of the HTS tape. Instead of using a current source, HTS flux pumps are capable of injecting flux into closed HTS magnets without electrical contact. This paper presents a practical superconducting DC dynamo for charging a conduction-cooled HTS magnet system based on a flux-pumping technique. To minimize heat losses, the rotor is driven by a servo motor mounted outside the vacuum dewar by utilizing magnetic fluid dynamic sealing. Different parameters, such as air gap and rotating speed, have been tested to investigate the best pumping effect, and finally, it successfully powers a 27.3 mH HTS non-insulated double-pancake coil to the current of 54.2 A within 76 min. As a low-cost and compact substitute for the traditional current source, the realization of a contactless DC power supply can significantly improve the flexibility and mobility of the HTS magnet system and could be of great significance for the technological innovation of future HTS magnets used in offshore wind turbines, biomedical, aerospace, etc.
Analysis and Modeling of the System Boundaries of a High-Speed Direct-Yarn-Placement System for In Situ Impregnation of Carbon Fibre Heavy Tows as Textile Reinforcements for Concrete Parts
Authors: Knoch Erik , Rittner Steffen , Holschemacher Klaus
Published: May 2024

This study investigates a novel approach in modeling the system limits of a braked, high-speed yarn-laying process with in situ impregnation. Special attention is paid to the investigation of the yarn spool overrun after the robot has come to a standstill. This phenomenon occurs at low yarn tensions in combination with high traversing speed and/or acceleration. The modeling of the yarn spool overrun is carried out using physical equations, taking into account the travel speed, acceleration of the robot, and braking force of the spool brake. Previous research has confirmed various operating points of the yarn-laying process, but a comprehensive and complete analysis of the system limits at different operating points and speeds up to 2 m/s is missing. The result of the study is a novel model that describes the system boundaries of the direct-yarn-placement. Furthermore, models for robot braking time, carbon spool diameter, and spool mass are developed. The proposed models have an R2 > 0.9674. Regarding the system stability boundaries, the calculations reveal that, as acceleration rises, the minimum tension requirement also increases. The same trend is found for system velocity. At a=12.5%, a minimum tension of 16 N suffices, compared to 23 N and 32 N at a=25% and 50%, respectively. The impact on tension of quadrupling the speed outweighs that of acceleration, with tension increasing by factors of up to 22.5 and 2, respectively.