bluuebunny/crossref_metadata_2025 huggingface dataset:
Altered cerebellar functional connectivity in chronic subcortical stroke patients
Authors: Hong Wenjun , Du Yilin , Xu Rong , Zhang Xin , Liu Zaixing , Li Ming , Yu Zhixuan , Wang Yuxin , Wang Minmin , Yang Bo , Sun Fenfen , Xu Guangxu
Published: November 2022

Background

Previous studies demonstrated that cerebellar subregions are involved in different functions. Especially the cerebellar anterior lobe (CAL) and cerebellar posterior lobe (CPL) have been postulated to primarily account for sensorimotor and cognitive function, respectively. However, the functional connectivity (FC) alterations of CAL and CPL, and their relationships with behavior performance in chronic stroke participants are unclear so far.

Materials and methods

The present study collected resting-state fMRI data from thirty-six subcortical chronic stroke participants and thirty-eight well-matched healthy controls (HCs). We performed the FC analysis with bilateral CAL and CPL as seeds for each participant. Then, we detected the FC difference between the two groups by using a two-sample t-test and evaluated the relationship between the FC and scores of motor and cognitive assessments across all post-stroke participants by using partial correlation analysis.

Results

The CAL showed increased FCs in the prefrontal cortex, superior/inferior temporal gyrus, and lingual gyrus, while the CPL showed increased FCs in the inferior parietal lobule, precuneus, and cingulum gyrus in the stroke participants compared with HCs. Moreover, the FC alteration in the right CAL and the right CPL were negatively correlated with executive and memory functions across stroke participants, respectively.

Conclusion

These findings shed light on the different increased FC alteration patterns of CAL and CPL that help understand the neuro-mechanisms underlying behavior performance in chronic stroke survivors.
Cannabis, connectivity, and coming of age: Associations between cannabis use and anterior cingulate cortex connectivity during the transition to adulthood
Authors: Lichenstein Sarah D. , Shaw Daniel S. , Forbes Erika E.
Published: November 2022

Cannabis use is common among adolescents and emerging adults and is associated with significant adverse consequences for a subset of users. Rates of use peak between the ages of 18–25, yet the neurobiological consequences for neural systems that are actively developing during this time remain poorly understood. In particular, cannabis exposure may interfere with adaptive development of white matter pathways underlying connectivity of the anterior cingulate cortex, including the cingulum and anterior thalamic radiations (ATR). The current study examined the association between cannabis use during adolescence and emerging adulthood and white matter microstructure of the cingulum and ATR among 158 male subjects enrolled in the Pitt Mother and Child Project, a prospective, longitudinal study of risk and resilience among men of low socioeconomic status. Participants were recruited in infancy, completed follow-up assessments throughout childhood and adolescence, and underwent diffusion imaging at ages 20 and 22. At age 20, moderate cannabis use across adolescence (age 12–19) was associated with higher fractional anisotropy (FA) of the cingulum and ATR, relative to both minimal and heavy adolescent use. Longitudinally, moderate and heavy extended cannabis use (age 12–21) was associated with reduced positive change in FA in the cingulum from age 20 to 22, relative to minimal use. These longitudinal results suggest that cannabis exposure may delay cingulum maturation during the transition to adulthood and potentially impact individuals’ functioning later in development.
Potential value of differentially expressed circular RNAs derived from circulating exosomes in the pathogenesis of rat spinal cord injury
Authors: Zan Chunfang , Li Jianan , Lin Fengsong , Wang Zengliang
Published: November 2022

Spinal cord injury (SCI) remains one kind of devastating neurological damage, and specific molecular mechanisms involved need to be understood deeply. Currently, circular RNAs (circRNAs), as a newly discovered type of non-coding RNAs (ncRNAs), have been under active investigation. Through functional interactions with disease-associated microRNAs (miRNAs), exosome-derived circRNAs have been extensively implicated in various organ pathogenesis. Nevertheless, the functional involvement of circulating circRNAs in SCI onset, progression as well as repair remains poorly explored until now. Of note, there still lacks clinical and experimental evidence in this regard. To obtain some relevant knowledge in this field, this study was originally designed to have a general overview of differentially expressed circRNAs derived from circulating exosomes in SCI rats in comparison with the control rats. It turned out that 709 types of downregulated circRNAs and 346 kinds of upregulated circRNAs were preliminarily screened out. Functional enrichment analyses including kyoto encyclopedia of genes and genomes (KEGG) pathway and gene ontology (GO) were performed to evaluate the possible biological functions of upregulated as well as downregulated circRNAs involved in SCI. Furthermore, five types of upregulated circulating circRNAs including chr4:208359914–208362182+, chr15:20088296–20092102+, chr1:175098934– 175134845–, chr1:175099657– 175128203–, and chr1:175104454– 175134845–, and plus five kinds of downregulated circulating circRNAs including chr11:74154652– 74159524–, chr12:45412398– 45412635–, chr7:137630261– 137648924–, chr6:6280974–6281188+, and chr4:225251864–225254087+, were verified through reverse transcription-polymerase chain reaction (RT-PCR). At last, taking these differentially expressed circRNAs in the center, the circRNA-miRNA-mRNA gene interaction network was constructed to predict the possible functionalities of circRNAs in SCI through anticipating specific interactive miRNAs, giving new insights into how circRNAs contribute to this pathological process. Taken together, these findings suggest the possible involvement and functional significance of circRNAs in SCI.
Caffeine consumption within British fencing athletes
Authors: Morris George Bowra , Manders Ralph Johannes Francisca , Hulton Andrew Thomas
Published: November 2022

The ergogenic effects of caffeine are well documented, yet despite the potential benefits of supplementation, there is a lack of understanding of caffeine habits and supplementation within fencing. British fencers (n = 136) completed a Web-based questionnaire, exploring self-reported caffeine consumption, reasons for use and education. Fencers (94.1%) habitually consumed caffeine, primarily due to the taste of the products (93.8%). Respondents ingested 183.4 ± 137.5 mg of caffeine daily, with a significant difference between age groups (p < 0.05). Many respondents (30.1%) consumed caffeine 60 mins prior/during fencing training and/or competition with the main reason highlighted as cognitive performance enhancement. Respondents ingested 140.8 ± 104.6 mg of caffeine during training/competition, mainly as energy drinks, bars, and powders. Education on caffeine supplementation was low (25.7%), with significant associations between age groups (p < 0.05). Evidence implies caffeine toxicity has been experienced by 35% of fencers, highlighting the need for education on caffeine consumption. To conclude there is evidence of caffeine supplementation in fencing, primarily to magnify cognitive performance. However, there is a requirement for targeted education on caffeine supplementation to fencers, so that negative side effects and potential anti-doping infringements can be avoided.
Microfluidic vascular models of tumor cell extravasation
Authors: Kim Seunggyu , Wan Zhengpeng , Jeon Jessie S. , Kamm Roger D.
Published: November 2022

Emerging microfluidic disease models have amply demonstrated their value in many fields of cancer research. These in vitro technologies recapitulate key aspects of metastatic cancer, including the process of tumor cell arrest and extravasation at the site of the metastatic tumor. To date, extensive efforts have been made to capture key features of the microvasculature to reconstitute the pre-metastatic niche and investigate dynamic extravasation behaviors using microfluidic systems. In this mini-review, we highlight recent microfluidic vascular models of tumor cell extravasation and explore how this approach contributes to development of in vitro disease models to enhance understanding of metastasis in vivo.
Quantifying developmental and individual differences in spontaneous drawing completion among children
Authors: Philippsen Anja , Tsuji Sho , Nagai Yukie
Published: November 2022

This study investigated how children's drawings can provide insights into their cognitive development. It can be challenging to quantify the diversity of children's drawings across their developmental stages as well as between individuals. This study observed children's representational drawing ability by conducting a completion task where children could freely draw on partially drawn objects, and quantitatively analyzed differences in children's drawing tendencies across age and between individuals. First, we conducted preregistered analyses, based on crowd-sourced adult ratings, to investigate the differences of drawing style with the age and autistic traits of the children, where the latter was inspired by reports of atypical drawing among children with autism spectrum disorder (ASD). Additionally, the drawings were quantified using feature representations extracted with a deep convolutional neural network (CNN), which allowed an analysis of the drawings at different perceptual levels (i.e., local or global). Findings revealed a decrease in scribbling and an increase in completion behavior with increasing age. However, no correlation between drawing behavior and autistic traits was found. The network analysis demonstrated that older children adapted to the presented stimuli in a more adult-like manner than younger children. Furthermore, ways to quantify individual differences in how children adapt to the presented stimuli are explored. Based on the predictive coding theory as a unified theory of how perception and behavior might emerge from integrating sensations and predictions, we suggest that our analyses may open up new possibilities for investigating children's cognitive development.
Ambidextrous human resource practices and individual creativity—A cross-layer multi-time analysis based on psychological capital and QLMX
Authors: Zhao Fuqiang , Wang Longdong , Chen Yun , Hu Wei
Published: November 2022

As an important factor in enhancing individual creativity, employees’ psychological capital has been widely tested by scholars. However, the effects of ambidextrous human resource practices (AHRP) on individual creativity has not been confirmed. On the basis of the theories of social exchange and resource preservation, we explored the mechanism of AHRP’s impact on individual creativity using three-point data collected from March to September 2021 from 23 large enterprises in the service, finance, construction, and education sectors in five Chinese cities: Wuhan, Shanghai, Guangdong, Shenzhen, and Zhengzhou. A cross-layer regression and a Monte Carlo sampling analysis were performed on 135 pairs of leaders and 810 employees. In addition, we tested the cross-layer mediating effect of the psychological capital between AHRP and individual creativity and the boundary effect of the quality of leader–member exchange (QLMX) in the cross-layer effect. Research results indicate that (1) AHRP have positive effects on individual creativity across layers; (2) psychological capital mediates the cross-layer relationship between AHRP and individual creativity; (3) QLMX moderates the direct effect of AHRP on employees’ psychological capital; and (4) QLMX moderates the indirect effect of AHRP on individual creativity through psychological capital. The research conclusions lay a theoretical foundation for AHRP at the organizational level and provide a guiding reference for the enhancement of employee creativity at the individual level.
Superordinate identities and self-transcendent emotions: Longitudinal study in Spain and Chile
Authors: Wlodarczyk Anna , Méndez Lander , Cusi Olaia , Telletxea Saioa , Mendia Jara , Briceño Mauricio , Delgado Daniela , Balbontín Francisca , Lecaros Alexandra , Páez Darío
Published: November 2022

Recent studies suggest that identification with all humanity (IWAH), apart from being related to universalistic values, could also be related to self-transcendent emotions (STE). In this scenario, the general objective of this cross-cultural longitudinal study is to examine the relationship between identification with proximate categories (i.e., community and country) and superordinate one (all humanity), and their association with positive self-oriented and STEs during a traumatic global phenomenon such as COVID-19 pandemics. Additionally, we explore variations regarding the patterns of those associations in different cultural contexts (Chile and Spain) and examine whether they change among two different time points (T1–T2). The total sample was composed of 403 participants, of whom 224 were residents in Chile (M = 39.25, SD = 12.56; range 18–71 years; 49.6% women) and 179 were residents in Spain (M = 36.35, SD = 12.12; range 18–68 years; 59.8% women). Data collection was carried out in September (T1) and November (T2) 2020, through online surveys administered via Survey Monkey® platform. Overall, results show, as expected, greater identification with proximate categories rather than superordinate ones, and an association between STEs and IWAH, but also with national and community identification. IWAH, but not STEs decreased significantly (T1–T2) in both countries. Thereafter, these emotional and behavioral responses decline as a symptom of growing fatigue with the pandemic situation, and also reflect a shift from broader to more local concerns. Analysis regarding comparisons between countries indicated higher levels of identification with community and with all humanity in Spain and with country in Chile. The results are discussed in the context of new developments in studies on IWAH.
Quantitative study of the hardening in the Alpha Magnetic Spectrometer nuclei spectra at a few hundred GV
Authors: Niu Jia-Shu , Liu Jing
Published: November 2022

The most significant feature in the cosmic-ray (CR) nuclei spectra is the spectral hardening at a few hundred GV. It is important to know whether the hardening of different nuclei species is the same or not for constructing CR sources and propagation models. In this work, we collect the recently released AMS-02 CR nuclei spectra of primary species (proton, helium, carbon, oxygen, neon, magnesium, silicon, and iron), secondary species (lithium, beryllium, boron, and fluorine), and hybrid species (nitrogen, sodium, and aluminum) and study the break positions and the spectral index differences (less and greater than the break rigidity) of the spectral hardening quantitatively. The results show us that the CR nuclei spectral hardening at a few hundred GV has hybrid origins. In detail, the dominating factors of the spectral hardening for primary and secondary CR nuclei species are different: the former comes from the superposition of different kinds of CR sources, while the latter comes from the propagation process. Both of these factors influence all kinds of CR nuclei spectra, just with different weights.
Jain point laparoscopic entry in contraindications of Palmers point
Authors: Jain Nutan , Srivastava Sakshi , Bayya Sri Lakshmi Prasanna , Jain Vandana
Published: November 2022

Background

This study was conducted to assess the efficacy of the Jain point to overcome the contraindications of Palmer's point. The Jain point lies on the left side of the abdomen at the L4 level, 10–13 cm lateral to the umbilicus. Due to its anatomical location, the Jain point is free from adhesions because postsurgical adhesions are encountered usually in the midline or the right side.

Methods

This is a retrospective study conducted at a high-volume tertiary care referral center for advanced gynecological laparoscopic surgery, enrolling 8,586 patients who underwent laparoscopy at the center from January 2011 to March 2022. In this paper, we analyze 2,519 patients with a history of previous surgeries, who were operated using the Jain point.

Results

In the 2,519 patients with a history of previous surgeries, the Jain point port was found to be adhesion free, regardless of the location of the scars, the number and type of previous surgeries, and those in whom Palmer's point was contraindicated. No major complications were reported, except for one case (0.04%) of small bowel injury, which was managed intraoperatively. The Jain point continued to function as the main ergonomic working port.

Conclusion

The Jain point offers an alternate safe entry port in previous surgery cases for laparoscopic surgeons of various specialties, like general surgeons, urologists, oncologists, and bariatric surgeons, to overcome the contraindications of Palmer's point. The Jain point also acts as the main ergonomic working port, whereas Palmer's point becomes redundant after initial entry.
A randomized, prospective, masked clinical trial comparing an opioid-free vs. opioid-sparing anesthetic technique in adult cats undergoing ovariohysterectomy
Authors: Rufiange Maxime , Ruel Helene L. M. , Monteiro Beatriz P. , Watanabe Ryota , Cruz Benedetti Inga-Catalina , Benito Javier , Steagall Paulo V. M.
Published: November 2022

This study aimed to compare the analgesic effects of an injectable protocol using multimodal analgesia with or without opioids in cats undergoing ovariohysterectomy (OVH). Thirty-two healthy cats were enrolled in a prospective, blinded, randomized trial after the caregiver's written consent. Cats received a combination of ketamine (4 mg/kg), midazolam (0.25 mg/kg) and dexmedetomidine (40 μg/kg), and either buprenorphine (20 μg/kg) or saline (same volume as buprenorphine) intramuscularly [opioid-sparing (OSA) and opioid-free anesthesia (OFA) groups, respectively]. Intraperitoneal bupivacaine 0.25% (2 mg/kg) and meloxicam (0.2 mg/kg subcutaneously) were administered before OVH. Atipamezole (400 μg/kg intramuscularly) was administered at the end of surgery. Pain and sedation were evaluated using the Feline Grimace Scale (FGS) and a dynamic interactive visual analog scale, respectively. Intravenous buprenorphine was administered as rescue analgesia if FGS scores ≥ 0.39/1. Statistical analysis included repeated measures linear mixed models, Fisher's exact test and Bonferroni adjustments when appropriate (p < 0.05). Twenty-seven cats were included. The prevalence of rescue analgesia was lower in OSA (n = 0/13) than in OFA (n = 5/14) (p = 0.04). The FGS scores (least square means and 95% CI) were higher in OFA at 1 [2.0 (1.3–2.7)] and 2 h [2.2 (1.5–2.9)] than baseline [0.7 (0.0–1.4)], but not in OSA. Sedation scores were not significantly different between groups. Opioid-free injectable anesthesia was appropriate for some cats using a multimodal approach. However, a single dose of intramuscular buprenorphine eliminated the need for rescue analgesia and assured adequate pain management after OVH in cats.
Hybrid Harmony Search for Stochastic Scheduling of Chemotherapy Outpatient Appointments
Authors: Corsini Roberto Rosario , Costa Antonio , Fichera Sergio , Parrinello Vincenzo
Published: November 2022

This research deals with the same-day chemotherapy outpatient scheduling problem that is recognized as a leading strategy to pursue the objective of reducing patient waiting time. Inspired by a real-world context and different from the other studies, we modeled a multi-stage chemotherapy ward in which the pharmacy is located away from the treatment area and drugs are delivered in batches. Processes in oncology wards are characterized by several sources of uncertainty that increase the complexity of the problem; thus, a stochastic approach was preferred to study the outpatient scheduling problem. To generate effective appointment schedules, we moved in two directions. First, we adopted a late-start scheduling strategy to reduce the idle times within and among the different stages, namely medical consultation, drug preparation and infusion. Then, since the problem is NP-hard in the strong sense, we developed a hybrid harmony search metaheuristic whose effectiveness was proved through an extended numerical analysis involving another optimization technique from the relevant literature. The outcomes from the numerical experiments confirmed the efficacy of the proposed scheduling model and the hybrid metaheuristic algorithm as well.
The Research on Soft Pneumatic Actuators in Italy: Design Solutions and Applications
Authors: Paterna Maria , De Benedictis Carlo , Ferraresi Carlo
Published: November 2022

Interest in soft actuators has increased enormously in the last 10 years. Thanks to their compliance and flexibility, they are suitable to be employed to actuate devices that must safely interact with humans or delicate objects or to actuate bio-inspired robots able to move in hostile environments. This paper reviews the research on soft pneumatic actuators conducted in Italy, focusing on mechanical design, analytical modeling, and possible application. A classification based on the geometry is proposed, since a wide set of architectures and manufacturing solutions are available. This aspect is confirmed by the extent of scenarios in which researchers take advantage of such systems’ improved flexibility and functionality. Several applications regarding bio-robotics, bioengineering, wearable devices, and more are presented and discussed.
Sex Detection of Chicks Based on Audio Technology and Deep Learning Methods
Authors: Li Zeying , Zhang Tiemin , Cuan Kaixuan , Fang Cheng , Zhao Hongzhi , Guan Chenxi , Yang Qilian , Qu Hao
Published: November 2022

The sex detection of chicks is an important work in poultry breeding. Separating chicks of different sexes early can effectively improve production efficiency and commercial benefits. In this paper, based on the difference in calls among one-day-old chicks of different sexes, a sex detection method based on chick calls is designed. Deep learning methods were used to classify the calls of chicks and detect their sex. This experiment studies three different varieties of chicks. The short-time zero-crossing rate was used to automatically detect the endpoints of chick calls in audio. Three kinds of audio features were compared: Spectrogram, Cepstrogram and MFCC+Logfbank. The features were used as the input in neural networks, and there were five kinds of neural networks: CNN, GRU, CRNN, TwoStream and ResNet-50. After the cross-comparison experiment of different varieties of chicks, audio features and neural networks, the ResNet-50 neural network trained with the MFCC+Logfbank audio features of three yellow chick calls had the highest test accuracy of 83% when testing Three-yellow chicks’ calls. The GRU neural network trained with the Spectrogram audio features of native chick calls had the highest test accuracy of 76.8% when testing Native chicks’ calls. The ResNet-50 neural network trained with Spectrogram audio features of flaxen-yellow chick calls had the highest test accuracy of 66.56%when testing flaxen-yellow chick calls. Multiple calls of each chick were detected, and the majority voting method was used to detect the sex of the chicks. The ResNet-50 neural network trained with the Spectrogram of three yellow chick calls had the highest sex detection accuracy of 95% when detecting the three yellow chicks’ sex. The GRU neural network trained with the Spectrogram and cepstrogram of native chick calls and the CRNN network trained with the Spectrogram of native chick calls had the highest sex detection accuracy of 90% when detecting the native chicks’ sex. The Twostream neural network trained with MFCC+Logfbank of flaxen-yellow chick calls and the ResNet-50 network trained with the Spectrogram of flaxen-yellow chick calls had the highest sex detection accuracy of 80% when detecting the flaxen-yellow chicks’ sex. The results of the cross-comparison experiment show that there is a large diversity between the sex differences in chick calls of different breeds. The method is more applicable to chick sex detection in three yellow chicks and less so in native chicks and flaxen-yellow chicks. Additionally, when detecting the sex of chicks of a similar breed to the training chicks, the method obtained better results, while detecting the sex of chicks of other breeds, the detection accuracy was significantly reduced. This paper provides further perspectives on the sex detection method of chicks based on their calls and help and guidance for future research.
Effects of Dietary Rapeseed (Brassica napus), Hemp (Cannabis sativa) and Camelina (Camelina sativa) Seed Cakes Supplementation on Yolk and Albumen Colour and Nutritional Value of Yolk Lipids in Estonian Quail Eggs
Authors: Razmaitė Violeta , Šiukščius Artūras , Leikus Raimondas
Published: November 2022

The aim of this study was to investigate the effects of supplementing commercial diets with rapeseed, hempseed and camelina seed cakes on the yolk and albumen colour and nutritional value of yolk lipids in Estonian quail eggs. A total of 585 eggs were used to evaluate egg weight, yolk and albumen pH and colour, proximate yolk composition, cholesterol content and fatty acid composition in the yolk lipids of three Estonian quail groups each fed a diet supplemented with 10% rapeseed, hemp or camelina seed cakes. The higher (p < 0.01 and p < 0.05, respectively) lightness (L*) of the yolk was detected in the group of quail fed the diet supplemented with rapeseed and camelina cakes compared to the hempseed addition. Meanwhile, the albumen lightness in the rapeseed group was higher (p < 0.01) in comparison to the camelina group. A lower (p < 0.001) yolk negative a* value was estimated in the hemp group than in the groups fed rapeseed and camelina seed cake supplements, while the rapeseed group demonstrated lower (p < 0.001) albumen yellowness (b*) and colour saturation (C) in comparison to the other groups. The highest and lowest proportions of total saturated (SFAs)and monounsaturated (MUFAs) fatty acids, respectively, were found in the yolk lipids of quails fed a mixture supplemented by hemp cake. A higher (p < 0.05) proportion of polyunsaturated fatty acids (PUFAs) was only found in the hemp group compared to the camelina group. The highest and lowest (p < 0.001) n-6/n-3 PUFA ratios were found in the yolk lipids of quail receiving mixtures supplemented with rapeseed and camelina cakes, respectively. The yolk of the quail from the rapeseed group showed more favourable lower and higher (p < 0.01 and p < 0.05, respectively) atherogenic index (AI) and hypocholesterolemic/hypercholesterolemic (h/H) ratio compared to the hemp and camelina groups. The lower yolk cholesterol content was found in the eggs of the quail fed the diet supplemented with rapeseed cake compared to the eggs of the quail which were fed the diets containing more n-3 PUFAs from the addition of hemp and camelina cakes (p < 0.05 and p < 0.01, respectively).
Using Cumulus Cell Biopsy as a Non-Invasive Tool to Access the Quality of Bovine Oocytes: How Informative Are They?
Authors: Sprícigo José Felipe Warmling , Guimarães Ana Luiza Silva , Cunha Andrielle Thainar Mendes , Leme Ligiane de Oliveira , Carneiro Marcos Coura , Franco Maurício Machaim , Dode Margot Alves Nunes
Published: November 2022

The present study aimed to determine whether cumulus cells (CC) biopsy, acquired before or after in vitro maturation (IVM), presents similar gene expression pattern and if would compromises oocyte quality. First, immature cumulus oocyte complexes (COCs) were distributed: (1) maturated in groups (control); (2) individually maturated, but not biopsied; (3) subjected to CC biopsy before maturation and individually matured; (4) individually matured and submitted to CC biopsy after maturation; (5) individually matured and CC biopsied before and after maturation. Secondly, candidate genes, described as potential markers of COCs quality, were quantified by RT-qPCR in CCs before and after IVM. After in vitro fertilization (IVF), zygotes were tracked and sorted regarding their developmental potential: fully developed to embryo, cleaved and arrested, and not-cleaved. The COC’s biopsy negatively affects embryo development (p < 0.05), blastocyst cell number (p < 0.05), and apoptotic cell ratio (p < 0.05), both before and after IVM. The PTGS2, LUM, ALCAM, FSHR, PGR, SERPINE2, HAS2, and PDRX3 genes were differentially expressed (p < 0.05) on matured CCs. Only PGR gene (p = 0.04) was under-expressed on matured CCs on Not-Cleaved group. The SERPINE2 gene was overexpressed (p = 0.01) in the Cleaved group on immature CCs. In summary, none of the selected gene studies can accurately predict COC’s fate after fertilization.
Impact of Climate Change and Drought Attributes in Nigeria
Authors: Ogunrinde Akinwale T. , Oguntunde Philip G. , Akinwumiju Akinola S. , Fasinmirin Johnson T. , Olasehinde David A. , Pham Quoc Bao , Linh Nguyen Thi Thuy , Anh Duong Tran
Published: November 2022

Data from historical observatories and future simulations were analyzed using the representative concentration pathway (RCP) 8.5 scenario, which covered the period from 1951 to 2100. In order to characterize the drought, three widely used drought indicators were used: the standardized precipitation index (SPI), the reconnaissance drought index (RDI), and the standardized precipitation and evapotranspiration index (SPEI). The ensemble of the seven (7) GCMs that used RCA-4 was able to capture several useful characteristics of Nigeria’s historical climatology. Future climates were forecasted to be wetter than previous periods during the study period based on the output of drought characteristics as determined by SPI. SPEI and RDI predicted drier weather, in contrast. SPEI and RDI’s predictions must have been based on the effect of rising temperatures brought on by global warming as depicted by RCP 8.5, which would then have an impact on the rate of evapotranspiration. According to drought studies using the RCP 8.5 scenario, rising temperatures will probably cause more severe/extreme droughts to occur more frequently. SPEI drought frequency changes in Nigeria often range from 0.75 (2031–2060) to 1.80 (2071–2100) month/year, whereas RDI changes typically range from 0.30 (2031–2060) to 0.60 (2071–2100) month/year. The frequency of drought incidence has recently increased and is now harder to forecast. Since the Sendai Framework for Disaster Risk Reduction 2015–2030 (SFDRR) and the Sustainable Development Goals (SDGs) have few more years left to be completed, drastic efforts must be made to create climate-resilient systems that can tackle the effects that climate change may have on the water resources and agricultural sectors.
Preliminary Findings on CO2 Capture over APTES-Modified TiO2
Authors: Wanag Agnieszka , Kapica-Kozar Joanna , Sienkiewicz Agnieszka , Rokicka-Konieczna Paulina , Kusiak-Nejman Ewelina , Morawski Antoni W.
Published: November 2022

In this work, the impact of TiO2 properties on the CO2 adsorption properties of titanium dioxide modified with 3-aminopropyltriethoxysilane (APTES) was presented. The APTES-modified TiO2 materials were obtained by solvothermal process and thermal modification in the argon atmosphere. The prepared adsorbents were characterized by various techniques such as X-ray diffraction (XRD), Fourier transform infrared (DRIFT), thermogravimetric analysis and BET specific surface area measurement. CO2adsorption properties were measured at different temperatures (0, 30, 40, 50 and 60 °C). Additionally, the carbon dioxide cyclic adsorption-desorption measurements were also investigated. The results revealed that modifying TiO2 with APTES is an efficient method of preparing CO2 sorbents. It was found that the CO2 adsorption capacity for the samples after modification with APTES was higher than the sorption capacity for unmodified sorbents. The highest sorption capacity reached TiO2-4 h-120 °C-100 mM-500 °C sample. It was also found that the CO2 adsorption capacity shows excellent cyclic stability and regenerability after 21 adsorption-desorption cycles.
What Differentiates Dielectric Oxides and Solid Electrolytes on the Pathway toward More Efficient Energy Storage?
Authors: Guerreiro Antonio Nuno , Maia Beatriz Arouca , Khalifa Hesham , Baptista Manuela Carvalho , Braga Maria Helena
Published: November 2022

Taking advantage of electrode thicknesses well beyond conventional dimensions allowed us to follow the surface plasmonic THz frequency phenomenon with vacuum wavelengths of 100 μm to 1 mm, only to scrutinize them within millimeters-thicknesses insulators. Here, we analyze an Al/insulator/Cu cell in which the metal electrodes-collectors were separated by a gap that was alternatively filled by SiO2, MgO, Li2O, Na3Zr2Si2PO12–NASICON, Li1.5Al0.5Ge1.5(PO4)3–LAGP, and Li2.99Ba0.005ClO–Li+ glass. A comparison was drawn using experimental surface chemical potentials, cyclic voltammetry (I-V plots), impedance spectroscopy, and theoretical approaches such as structure optimization, simulation of the electronic band structures, and work functions. The analysis reveals an unexpected common emergency from the cell’s materials to align their surface chemical potential, even in operando when set to discharge under an external resistor of 1842 Ω.cminsulator. A very high capability of the metal electrodes to vary their surface chemical potentials and specific behavior among dielectric oxides and solid electrolytes was identified. Whereas LAGP and Li2O behaved as p-type semiconductors below 40 °C at OCV and while set to discharge with a resistor in agreement with the Li+ diffusion direction, NASICON behaved as a quasi n-type semiconductor at OCV, as MgO, and as a quasi p-type semiconductor while set to discharge. The capacity to behave as a p-type semiconductor may be related to the ionic conductivity of the mobile ion. The ferroelectric behavior of Li2.99Ba0.005ClO has shown surface plasmon polariton (SPP) waves in the form of surface propagating solitons, as in complex phenomena, as well as electrodes’ surface chemical potentials inversion capabilities (i.e., χ (Al) − χ (Cu) > 0 to χ (Al) − χ (Cu) < 0 vs. Evacuum = 0 eV) and self-charge (ΔVcell ≥ +0.04 V under a 1842 Ω.cminsulator resistor). The multivalent 5.5 mm thick layer cell filled with Li2.99Ba0.005ClO was the only one to display a potential bulk difference of 1.1 V. The lessons learned in this work may pave the way to understanding and designing more efficient energy harvesting and storage devices.
Bilayers as Basic Formation of Epimolecular Structure of Mostly Lyotropic (Hydrotropic) Structuralized Liquid Systems Being Influenced Predominantly by the Temperature
Authors: Milichovský Miloslav
Published: November 2022

The bilayer’s formations of amphiphilic molecules or polyions of different ionogenity comprise the basic building units of most organic biological and non-biological systems. A theory has evolved to explain their behaviour during the creation of those organized structures, such as anisotropic liquid crystal (LC) in lyotropic (especially hydrotropic) systems and polyelectrolyte multilayer (PEM) assemblies. Particular attention has been paid to the temperature and the important role of water in the formation and behaviour of the bilayers. A novel insight into the formation of hydrotropic liquid LC systems and their thermotropic behaviour is presented. In this context, the systems PEM assemblies are also discussed. Essentially, a structuralised form of water fills out continuous and discontinuous, i.e., confined, nano-spaces among hydrophilic interfaces of bilayers, controlling their supramolecular structure through a system of attractive and repulsive hydration forces. The character of those sophisticated bonding hydration systems is predestined by the composition and type of these hydrophilic interface groups. The miscellaneous complexity of the bilayer’s aqueous systems suggests the need to study these examples in greater detail. Therefore, the bilayer’s processes connected with disruption as far as destruction of bilayers are mentioned, i.e., the processes with the highest potential to combat bacteria, fungi, and viruses, such as in a situation where a person exhales a breath of micro-droplets containing virus nanoparticles (e.g., the COVID-19 virus).
The Effect of Intraocular Pressure Load Boundary on the Biomechanics of the Human Conventional Aqueous Outflow Pathway
Authors: Karimi Alireza , Razaghi Reza , Rahmati Seyed Mohammadali , Downs J. Crawford , Acott Ted S. , Kelley Mary J. , Wang Ruikang K. , Johnstone Murray
Published: November 2022

Background: Aqueous humor outflow resistance in the trabecular meshwork (TM), juxtacanalicular connective tissue (JCT), and Schlemm’s canal (SC) endothelium of the conventional outflow pathway actively contribute to intraocular pressure (IOP) regulation. Outflow resistance is actively affected by the dynamic outflow pressure gradient across the TM, JCT, and SC inner wall tissues. The resistance effect implies the presence of a fluid–structure interaction (FSI) coupling between the outflow tissues and the aqueous humor. However, the biomechanical interactions between viscoelastic outflow tissues and aqueous humor dynamics are largely unknown. Methods: A 3D microstructural finite element (FE) model of a healthy human eye TM/JCT/SC complex was constructed with elastic and viscoelastic material properties for the bulk extracellular matrix and embedded elastic cable elements. The FE models were subjected to both idealized and a physiologic IOP load boundary using the FSI method. Results: The elastic material model for both the idealized and physiologic IOP load boundary at equal IOPs showed similar stresses and strains in the outflow tissues as well as pressure in the aqueous humor. However, outflow tissues with viscoelastic material properties were sensitive to the IOP load rate, resulting in different mechanical and hydrodynamic responses in the tissues and aqueous humor. Conclusions: Transient IOP fluctuations may cause a relatively large IOP difference of ~20 mmHg in a very short time frame of ~0.1 s, resulting in a rate stiffening in the outflow tissues. Rate stiffening reduces strains and causes a rate-dependent pressure gradient across the outflow tissues. Thus, the results suggest it is necessary to use a viscoelastic material model in outflow tissues that includes the important role of IOP load rate.
From Promise to Reality: Bioengineering Strategies to Enhance the Therapeutic Potential of Extracellular Vesicles
Authors: de Almeida Fuzeta Miguel , Gonçalves Pedro P. , Fernandes-Platzgummer Ana , Cabral Joaquim M. S. , Bernardes Nuno , da Silva Cláudia L.
Published: November 2022

Extracellular vesicles (EVs) have been the focus of great attention over the last decade, considering their promising application as next-generation therapeutics. EVs have emerged as relevant mediators of intercellular communication, being associated with multiple physiological processes, but also in the pathogenesis of several diseases. Given their natural ability to shuttle messages between cells, EVs have been explored both as inherent therapeutics in regenerative medicine and as drug delivery vehicles targeting multiple diseases. However, bioengineering strategies are required to harness the full potential of EVs for therapeutic use. For that purpose, a good understanding of EV biology, from their biogenesis to the way they are able to shuttle messages and establish interactions with recipient cells, is needed. Here, we review the current state-of-the-art on EV biology, complemented by representative examples of EVs roles in several pathophysiological processes, as well as the intrinsic therapeutic properties of EVs and paradigmatic strategies to produce and develop engineered EVs as next-generation drug delivery systems.
Therapy of PsO in Special Subsets of Patients
Authors: Di Cesare Antonella , Ricceri Federica , Rosi Elia , Fastame Maria Thais , Prignano Francesca
Published: November 2022

Psoriasis is a chronic, inflammatory skin disease that may occur at any age, with a bimodal peak of incidence around the age of 16–20 years of age (early onset) and 57–60 years (late-onset). It is estimated that roughly 70% of patients develop the disease before the age of 40, which coincides with the reproductive years. Moreover, psoriasis is a chronic disease, meaning that, with increased life-duration expectancy, the number of patients affected with psoriasis aged over 65 years is going to increase and represent a big therapeutic challenge. Actually, no specific drug recommendation is available, based only on the age of the patients, while therapeutic prescription should take into account that elderly patients have more comorbidities than younger patients, with polypharmacy and an increased risk of drug interactions. Women with psoriasis are more likely to report a worse influence of the disease on their quality of life, and they are more susceptible to the development of depression. Furthermore, pregnancy and lactation represent a major contraindication to several systemic agents, and only a few studies exist providing the safety of certain drugs during these periods of life of a woman, such as certolizumab pegol. In this paper, we discuss systemic therapeutic strategies, including conventional and biological therapies, in a special subset of patients affected with moderate-to-severe psoriasis focusing on elderly patients and on female patients in fertile age, pregnancy, and lactation.
Arrhythmic Burden in Cardiac Amyloidosis: What We Know and What We Do Not
Authors: Argirò Alessia , Del Franco Annamaria , Mazzoni Carlotta , Allinovi Marco , Tomberli Alessia , Tarquini Roberto , Di Mario Carlo , Perfetto Federico , Cappelli Francesco , Zampieri Mattia
Published: November 2022

Cardiac amyloidosis (CA), caused by the deposition of insoluble amyloid fibrils, impairs different cardiac structures, altering not only left ventricle (LV) systo-diastolic function but also atrial function and the conduction system. The consequences of the involvement of the cardiac electrical system deserve more attention, as well as the study of the underlying molecular mechanisms. This is an issue of considerable interest, given the conflicting data on the effectiveness of conventional antiarrhythmic strategies. Therefore, this review aims at summarizing the arrhythmic burden related to CA and the available evidence on antiarrhythmic treatment in this population.
Investigation of hs-TnI and sST-2 as Potential Predictors of Long-Term Cardiovascular Risk in Patients with Survived Hospitalization for COVID-19 Pneumonia
Authors: Fiedler Lukas , Motloch Lukas J. , Jirak Peter , Gumerov Ruslan , Davtyan Paruir , Gareeva Diana , Lakman Irina , Tataurov Alexandr , Lasinova Gulnaz , Pavlov Valentin , Hauptmann Laurenz , Kopp Kristen , Hoppe Uta C. , Lichtenauer Michael , Pistulli Rudin , Dieplinger Anna-Maria , Zagidullin Naufal
Published: November 2022

Introduction: COVID-19 survivors reveal an increased long-term risk for cardiovascular disease. Biomarkers like troponins and sST-2 improve stratification of cardiovascular risk. Nevertheless, their prognostic value for identifying long-term cardiovascular risk after having survived COVID-19 has yet to be evaluated. Methods: In this single-center study, admission serum biomarkers of sST-2 and hs-TnI in a single cohort of 251 hospitalized COVID-19 survivors were evaluated. Concentrations were correlated with major cardiovascular events (MACE) defined as cardiovascular death and/or need for cardiovascular hospitalization during follow-up after hospital discharge [FU: 415 days (403; 422)]. Results: MACE was a frequent finding during FU with an incidence of 8.4% (cardiovascular death: 2.8% and/or need for cardiovascular hospitalization: 7.2%). Both biomarkers were reliable indicators of MACE (hs-TnI: sensitivity = 66.7% & specificity = 65.7%; sST-2: sensitivity = 33.3% & specificity = 97.4%). This was confirmed in a multivariate proportional-hazards analysis: besides age (HR = 1.047, 95% CI = 1.012–1.084, p = 0.009), hs-TnI (HR = 4.940, 95% CI = 1.904–12.816, p = 0.001) and sST-2 (HR = 10.901, 95% CI = 4.509–29.271, p < 0.001) were strong predictors of MACE. The predictive value of the model was further improved by combining both biomarkers with the factor age (concordance index hs-TnI + sST2 + age = 0.812). Conclusion: During long-term FU, hospitalized COVID-19 survivors, hs-TnI and sST-2 at admission, were strong predictors of MACE, indicating both proteins to be involved in post-acute sequelae of COVID-19.
Impact of Scala Tympani Geometry on Insertion Forces during Implantation
Authors: Hrncirik Filip , Roberts Iwan V. , Swords Chloe , Christopher Peter J. , Chhabu Akil , Gee Andrew H. , Bance Manohar L.
Published: November 2022

(1) Background: During a cochlear implant insertion, the mechanical trauma can cause residual hearing loss in up to half of implantations. The forces on the cochlea during the insertion can lead to this mechanical trauma but can be highly variable between subjects which is thought to be due to differing anatomy, namely of the scala tympani. This study presents a systematic investigation of the influence of different geometrical parameters of the scala tympani on the cochlear implant insertion force. The influence of these parameters on the insertion forces were determined by testing the forces within 3D-printed, optically transparent models of the scala tympani with geometric alterations. (2) Methods: Three-dimensional segmentations of the cochlea were characterised using a custom MATLAB script which parametrised the scala tympani model, procedurally altered the key shape parameters (e.g., the volume, vertical trajectory, curvature, and cross-sectional area), and generated 3D printable models that were printed using a digital light processing 3D printer. The printed models were then attached to a custom insertion setup that measured the insertion forces on the cochlear implant and the scala tympani model during a controlled robotic insertion. (3) Results: It was determined that the insertion force is largely unaffected by the overall size, curvature, vertical trajectory, and cross-sectional area once the forces were normalised to an angular insertion depth. A Capstan-based model of the CI insertion forces was developed and matched well to the data acquired. (4) Conclusion: By using accurate 3D-printed models of the scala tympani with geometrical alterations, it was possible to demonstrate the insensitivity of the insertion forces to the size and shape of the scala tympani, after controlling for the angular insertion depth. This supports the Capstan model of the cochlear implant insertion force which predicts an exponential growth of the frictional force with an angular insertion depth. This concludes that the angular insertion depth, rather than the length of the CI inserted, should be the major consideration when evaluating the insertion force and associated mechanical trauma caused by cochlear implant insertion.