bluuebunny/crossref_metadata_2025 huggingface dataset:
Nomograms for Predicting Overall and Progression-Free Survival in Patients with Surgical Lip Squamous Cell Carcinoma
Authors: Saad Hassan A. , Baz Azza , Riad Mohamed , Eraky Mohamed E , El-Taher Ahmed k , Farid Mohamed I , Sharaf Khaled , Said Huda E. M.
Published: October 2023

Context
Receiving a personalized forecast for lip squamous cell carcinoma (LSCC) is crucial. This study aimed to develop predictive models for postoperative prognosis prediction in LSCC patients.
Methods
Two medical institutes provided 136 postoperative LSCC patients diagnosed between April 2015 and July 2022. The patients were randomly assigned to training and validation groups at a 7:3 ratio. Based on the results of univariate and multivariate Cox regression analyses, two nomograms were made to predict overall survival (OS) and progression-free survival (PFS) in LSCC patients who had surgery.
Results
According to univariate and multivariate Cox regression analyses, a lower pathological grade, being older than 70 years, having a higher T-stage, positive lymph node metastasis (LNM), perineural invasion (PNI), vascular invasion (VI), and positive surgical margin were all independent predictors of a lower OS. The independent predictors of worse PFS included higher pathological grade, age ≥ 70 years, higher T-stage, LNM, PNI, and positive surgical margins. Two nomograms were created based on the data above to forecast the 3- and 5-year OS and PFS rates in patients with LSCC. In the training group, the C-indices for the OS and PFS nomograms were 0.865 and 0.801, respectively, whereas those in the validation group were 0.915 and 0.815, respectively. The calibration curves demonstrated a reasonable degree of agreement between the observed survival rates and the predictions.
Conclusions
Curve analysis revealed superior performance of the nomogram compared to other related predictors. Kaplan-Meier curves showed statistical distinctions between the high-, medium-, and low-risk groups. In summary, With a good clinically personalized applicability value, this retrospective study developed and validated two nomograms for predicting OS and PFS in patients with postoperative LSCC.
The Role of Spirituality in Pain Experiences among Adults with Cancer: An Explanatory Sequential Mixed Methods Study
Authors: Miller Megan , Speicher Stephanie , Hardie Katie , Brown Roger , Rosa William E.
Published: October 2023

Purpose
Foundational research demonstrates that spirituality may affect the way people with cancer experience pain. One potential route is through alterations in thoughts and beliefs, such as pain-related catastrophizing. The purpose of this study is to understand whether spirituality impacts pain experiences through pain-related catastrophizing.
Methods
This explanatory sequential mixed methods study was informed by an adapted Theory of Unpleasant Symptoms. Data were collected via online surveys (N = 79) and follow-up qualitative interviews (N = 25). Phase 1 employed Empirical Bayesian analysis. Phase 2 used deductive content analysis. Phase 3 involved creating a mixed methods joint display to integrate findings and draw meta inferences.
Results
Results indicate that spirituality was directly negatively associated with pain-related catastrophizing, and indirectly negatively associated with the outcomes of pain interference, pain severity, and pain-related distress. Qualitative categories highlight the supportive role of spirituality when facing pain, while also shedding light on the limitations of spirituality in the context of some pain (i.e., severe, neuropathic, and/or chronic). Mixed methods findings reveal the importance of spirituality for some people as they face cancer and cancer-related pain, as well as the need for integrating spirituality as part of a larger pain management plan.
Conclusions
This research advances supportive cancer care by exploring the complex role of spirituality in pain experiences. Findings will inform further exploration into the role of spirituality in supporting holistic symptom management in the context of cancer, as well as developing and testing interventions to enhance spirituality and address symptom-related suffering.
The relationship between depression and mobile phone addiction in university students: the mediating role of anxiety and self-esteem
Authors: Dou Xiaofeng , Lu Jiachen , Yu Yingying , Yi Yaohui , Zhou Ling
Published: October 2023

Purpose
Cell phone addiction has become a widespread social phenomenon in China. Because this phenomenon has caused a wide range of social concerns, this study aims to explore the relationships among depression, anxiety, self-esteem, and cell phone addiction. It further aims to conduct an analysis of the factors affecting cell phone addiction.
Method
This study was conducted in 2023. We used the SDS depression scale, the SAS anxiety scale, the SAS cell phone addiction scale and the Robert self-esteem scale. Pearson correlation and structural equation modeling were used to explore the relationships among depression, anxiety, self-esteem, and cell phone addiction.
Results
The study's findings suggest that there is a correlation between depression, anxiety, self-esteem, and cell phone addiction. Additionally, self-esteem mediates the relationship between anxiety and cell phone addiction. Lastly, in the development of cell phone addiction, anxiety and self-esteem have sequential mediating roles.
Conclusion
These findings offer insight into the underlying mechanisms of the relationship between depression and cell phone addiction, thereby helping researchers and educators. In addition, the results provide practical and effective suggestions for the prevention and intervention of cell phone addiction among college students.
Comprehensive stereo monitoring and traceability analysis of ozone pollution: A case study of a continuous pollution process in Ganzhou City
Authors: Ma Fei , Wang Ming , Ye Conglei , Deng Xu , Zhang Shuai
Published: October 2023

The processes of O3 source and sink are complicated, and few existing studies focus on Ganzhou City, leading to a limited understanding of O3 pollution in Ganzhou City or even Jiangxi Province. Here, we investigate the comprehensive formation mechanism of O3 pollution from the aspects of precursor emission, chemical transformation, meteorological effects and three-dimensional transmission by using the vertical profiles for O3 concentration and wind information, ground monitoring data of ambient air quality, meteorological and volatile organic compounds (VOCs). The mean concentrations of O3, NO2 and TVOC shows significant growth in the early stage of O3 pollution, while the VOCs components is basically the same and the concentration proportion of the average VOCs concentration in TVOC and the proportion of the average O3 formation potential (OFP) concentration in the total OFP concentration did not change significantly before and after O3 pollution. The frequent high-altitude subsidence of O3 air mass, the night-time residue and the superposition of large-area transmission result in the characteristics of multi-peak and flat-headed peaks on the daily variation curve of O3, which is not conducive to the calculation of O3-8H concentration and are important reasons for O3 exceeding the standard. Meanwhile, the meteorological conditions like wind velocity and temperature may also influence the variations in the O3 concentration. From the point of view of air pollution prevention, the industrial enterprises and mobile sources may still be the vital industry of VOCs supervision and propional, 1-pentene, m-/p-xylene and isoprene could be considered as the important species under emergency management on O3 polluted days due to their significant increasing in OFP concentrations.
Might low-level laser therapy interfere with the frequency of severe mucositis and oral candidiasis? A retrospective analysis in patients with head and neck carcinoma
Authors: Nascimento Rebeca Barros , Neri Bruna Portle , Moutinho Mylena Melo , Fortuna Tila , Martins Gabriela Botelho , Carrera Manoela , Lima Hayana Ramos
Published: October 2023

Purpose
Considering the tumor in the oral cavity or the oropharynx and nasopharynx region might be an aggravating factor for Oral Mucositis (OM) manifestation, the present study aimed to evaluate whether the location of the tumor and the use of Low-level laser therapy (LLLT) might affect the frequency of oral candidiasis during radiotherapy (RT) and/or chemotherapy (CT) treatments.
Methods
Seventy-four patients with head and neck cancer were evaluated from 2016 to 2019. All these patients were submitted to RT in an accumulated dose of 48 to 70 Gy of radiation. These patients were followed with OM and OC treatment and divided into two groups: No-LLLT (SHAM group) and LLLT (Laser group). The correlation between clinical characteristics such as age, tumor size (T), metastatic lymph node (N), number of RT and CT sessions, candidiasis, and OM were analyzed.
Results
Mucositis grades 1 and 2 were the most common among all patients, especially before the 12th radiotherapy session, regardless of the treatment with LLLT. Additionally, no difference in the grade of OM and OC was significantly observed when comparing the two laser therapy groups. OC was more frequent after the 12th radiotherapy session in all groups.
Conclusion
OM and OC was not changed by LLLT, although it helped to reduce the incidence of severe cases of OM. Patients with oral cavity tumor presented more OM, especially high grades, then patients with tumors in other regions of the head and neck, which seems to be related to the irradiation parameters of radiotherapy and/or with the limitation of conduction of LLLT in tumor areas.
Neutrophil extracellular trap formation and deoxyribonuclease I activity in patients with otitis media with antineutrophil cytoplasmic antibody-associated vasculitis
Authors: Morita Shinya , Nakamaru Yuji , Fukuda Atsushi , Fujiwara Keishi , Suzuki Masanobu , Hoshino Kimiko , Honma Aya , Nakazono Akira , Homma Akihiro
Published: October 2023

No previous studies focused on the degradation of neutrophil extracellular traps (NETs) or deoxyribonuclease (DNase) I activity in the pathogenesis of otitis media with antineutrophil cytoplasmic antibody-associated vasculitis (OMAAV). The aim of this study was to explore the formation and degradation of NETs in the middle ear of patients with OMAAV during the onset and remission phases of the disease, with a particular focus on the relationships between the quantifiable NETs levels and DNase I activity. OMAAV patients were eligible for inclusion. Patients with otitis media with effusion (OME) were examined as controls. The levels of cell-free deoxyribonucleic acid (DNA), citrullinated-histone H3 (cit-H3)-DNA complex and myeloperoxidase (MPO)-DNA complex were quantified using an enzyme-linked immunosorbent assay. DNase I activity was measured using a fluorometric method. The quantifiable levels of cell-free DNA, cit-H3-DNA complex and MPO-DNA complex in the middle ear lavage of patients with OMAAV at onset were significantly higher than those in patients with OMAAV at remission and in patients with OME. DNase I activity in the patients with OMAAV at onset was significantly lower than those in patients with OMAAV at remission and OME, and was negatively correlated with the level of MPO-DNA complex. This study suggests that excessive NET formation and impaired DNase I activity are involved in the pathogenesis of OMAAV. NETs and DNase I activity may be useful biomarkers for the diagnosis and disease activity of OMAAV.
A Greener Biocatalyst promoted Synthesis of Novel pyridine-based Schiff base: Synthesis of their metal complex; In silico molecular docking and In-vitro biological studies and its Photocatalytic dye degradation
Authors: Unjiya Parth , Patel Umang , Rathod Vaishali , Mamtora Mayank , Parmar Hemal , Vaghela Foram , Solanki Mitesh , Shah Manish K.
Published: October 2023

In the present study a series of new Pyridine based schiff bases were synthesized by natural acid that was extracted from certain citrus fruits. By using a metal salt of NiCl2×6H2O, CoCl2×6H2O and Na2PdCl4 to synthesized a metal complexes. Structure of these compounds were confirmed by standard study of MS, LC-MS, FTIR, 1H NMR, 13C NMR, TGA, UV-spectroscopy. Antibacterial activity of synthesized molecules was tested against Gram-positive (S. aureus and E. faecalis) and Gram-negative (E. coli and P. aeruginosa) bacterial strains. Co (II), Ni (II) and Pd (II) metal complexes were used for the photocatalytic degradation of Ramazol brilliant blue R (RBBR) dye with the help of UV visible spectroscopy. The staphyloma concourses microbial structure was pre-processed for docking study using the AutoDock-Vina. The mentioned green chemistry techniques are economical, easy to understand, and give more effective extraction while showing maximum efficiency.
Serum exosomes of pregnant and non-pregnant buffaloes revealed protein signatures of conceptus development and events of the non-fertile cycle
Authors: V.R. Jithil , D.T. Vishruta , Tatu Utpal , Dhara Sujoy K. , Ghosh Subrata , Ghosh Jyotirmoy
Published: October 2023

Exosomes are membrane-bound extracellular vesicles (EVs) that carry proteins, nucleic acids, and lipids in their cargo from the cells of origin. They are detected in circulatory body fluids and are indicated to play significant roles during the establishment of pregnancy in humans and animals. This study aimed to isolate, quantify, profile, compare, and identify the elected differentially expressed serum exosome proteins in cycling and early-pregnant buffaloes. Serum exosomes were isolated from 0.5 mL serum of non-pregnant day 0, day 10, and day 15 of the cycle (n = 6), and early pregnancy days 15, day 30, and day 60 (n = 5) buffaloes by precipitation method. The proteins of serum exosomes were harvested by sonication, passing through Sephadex G25 spin columns, quantified, and analyzed by one-(1D) and two-dimensional (2D) SDS-PAGE gel electrophoresis. Selected differentially expressed proteins were analyzed by mass spectrometry and generated peptide mass (m)/charge (z) ratio searched against NCBI cattle and buffalo database to identify the protein. The results indicated serum exosome protein on cycle day 10 was significantly lower than the tested other days of the cycle and early pregnancy samples. The 1D electrophoresis did not reveal any difference but the 2D analysis revealed subtle differences. Mass spectrometry analysis of 9 up-regulated spots (8 pregnancy-specific and one cycle-specific) identified 19 proteins under 11 categories by database search. They were functionally related to mitosis, cell cycle regulation, morphogenesis, and regulation of several cellular and molecular pathways which are essential for conceptus development. Out of 19 proteins 17 were found functionally interconnected due to their common properties indicating their importance during the period of critical period of cycle and early pregnancy. In summary, serum exosomes of pregnant and non-pregnant buffaloes revealed protein signatures of conceptus development and events of the non-fertile cycle.
An Assessment of Marine Turtle Nesting Rookeries in the Southern Atlantic Coastline of Cameroon: Implications for Management and Conservation
Authors: Abi Henry Nibam , Avin Ohiana.Revuelta , Fretey Jacques , Tomas Jesus
Published: October 2023

In the present study, we evaluate for the first time marine turtle nesting activity on the coastal beaches of Ebodje, South Region of Cameroon. Regular surveys were carried out over 27 km of coastline in 2013 and over a lesser zone of the studied area with less survey effort from 2014 to 2017. Three marine turtle species were detected nesting in these beaches: the olive ridley (Lepidochelys olivacea), the leatherback turtle (Dermochelys coriacea), and more rarely the green turtle (Chelonia mydas). According to interviews with local inhabitants, the coastal waters of this area are also important for juvenile green turtles and hawksbills (Eretmochelys imbricate) on feeding grounds. Nesting activity concentrates between October and March, with very rare nesting activity detected out of this period. In four nesting seasons, we detected a total of 310 nesting activities, from which 179 clutches were successful; olive ridley was by far the most abundant species (164 clutches), followed by leatherback (11 clutches) and green turtle (4 clutches). The estimated number of olive ridleys in the first nesting season ranged from 30 to 46 turtles. We included information on turtle biometrics and clutch parameters for comparison with closer nesting roockeries and contextualized them with information on human-related threats. Detected threats facing the species were bycatch in artisanal fisheries, beach erosion, light pollution in some beaches, and illegal harvesting of eggs and nesting females at beaches. To mitigate some of these threats, a hatchery was developed on the beach with the highest density of nesting activity. Although there is scarce information about southern Cameroon marine turtle nesting stocks, the levels of nesting and the information on anthropogenic threats reported here indicate that this breeding aggregation is a serious threat and that conservation initiatives are mandated here.
Evaluation of led light on transgenic Nicotiana benthamiana production of a recombinant antibody fragment scFvanti-BAP1.
Authors: Gomes Marinna , Aragão Matheus , Pereira Hyago , Mattos Elyabe , Peixoto Paulo , Martins Marta , Santos Marcelo
Published: October 2023

Plants are an important alternative to produce monoclonal antibodies (mAbs). Although, the plant-based platform still faces several hurdles, that include the choice of plant species to be transformed, the development of suitable plant culture conditions, the genetic lineage of the plant, and the prevention of gene silencing. All of these challenges hinder the enhancement of recombinant productivity. Considering all steps in mAb production, its costs per protein gram are equivalent to that obtained using Chinese hamster ovary cells (CHO cells). Therefore, the construction of a system that optimizes some of these parameters would be welcome. Thus, the development of systems that increase protein production in plant cells are welcome. In the present work, we evaluated three different lineages of a transgenic plant producing mAb scFv-anti-BAP1 under different light conditions. Morphological characteristics, gDNA level and quantitative gene expression were monitored to evaluate the light effects in recombinant protein production. We found that White/Deep Red light increases the total soluble protein production in plants, as well as recombinant yield.
The causal relationship between C-reactive protein and idiopathic pulmonary fibrosis: A univariate and multivariate Mendelian randomization study
Authors: Yang Qiuping , Chen Xuefen , Zhang Yuhhua , Wu Qi
Published: October 2023

Background
This study employs univariate and multivariate Mendelian randomization designs, utilizing publicly available Genome-Wide Association Study (GWAS) data, to assess the causal relationship between C-reactive protein (CRP) and idiopathic pulmonary fibrosis (IPF).
Methods
We obtained IPF GWAS summary statistics (1028 cases and 196,986 controls) from the FinnGen Consortium (r5) and CRP, BMI, and ever-smoked summary statistics from IEU. The primary approach used was the inverse-variance weighted (IVW) method, supplemented by MR-Egger, weighted median and MR-PRESSO methods. Sensitivity analyses, including tests for heterogeneity, horizontal pleiotropy, and leave-one-out analysis, were also conducted.
Results
Mendelian Randomization (MR) analysis reveals a significant causal effect of genetically predicted CRP on IPF (IVW: OR 1.446, 95%CI 1.128–1.854, P = 0.004). This association remains significant after adjusting for BMI and smoking (OR 1.533, 95%CI 1.194–1.96, P < 0.001; OR 1.432, 95%CI 1.129–1.817, P = 0.003, respectively), with no evidence of heterogeneity or horizontal pleiotropy. Sensitivity analyses confirm the stability and reliability of our findings.
Conclusion
Our findings support a causal relationship between CRP and IPF, emphasizing the importance of monitoring femoral neck IPF in patients with chronic inflammatory conditions. Further research is needed to validate our conclusions and elucidate the underlying biological mechanisms. These discoveries provide valuable insights into the pathogenesis and potential future therapies for IPF.
Avoidant/Restrictive Food Intake Disorder and Psychiatric Comorbidity: a descriptive cross sectional study of a tertiary care hospital
Authors: Rodrigues Márcia , Sá Teresa , Mansilha Helena
Published: October 2023

Background: Avoidant/restrictive Food Intake Disorder (ARFID) is a heterogeneous psychiatric disorder that describes a group of patients with avoidant or restrictive eating behaviors unrelated to body image disorder or weight loss desire. Our purpose is to characterize clinical and psychiatric dimensions in children and adolescents diagnosed with ARFID.
Methods: A descriptive cross-sectional study was completed on patients under 17-years and 364 days that were assessed in a specialized consultation at the Nutrition Unit of CMIN between january 2023 and june 2023.
Results: Of the 46 children and adolescents diagnosed of ARFID, 60.9% (n=28), were male and the mean age of our population was 6.3 years (SD=3.8; range=0.8–15.9 years). Most patients presented symptoms from an early age: especially between 18 and 24 months (23.9%, n=11). The mean duration of symptoms prior to diagnosis of ARFID at the Nutrition Unit was 51.8 months (SD = 39.6; range = 2–158 months). The groupings of the ARFID patients included 71.9% (n=34) with difficulties associated with the sensory characteristics of qualities of food; 17.4% (n=8) with lack of interest in eating or food and 8.7% (n=4) with a previously conditioned negative response associated with food intake. In terms of psychiatric comorbidity, 82.6 % (n=38) met additional inclusion criteria for other formal Diagnostic and Statistical Manual of mental disorders 5th edition, text revision (DSM-5-TR) comorbid psychiatric diagnosis. Of these, 63.2% (n=24) had two or more comorbid diagnoses. The most frequent comorbidity was with Autism Spectrum Disorder plus Global Developmental Delay which occurred in 23.7% (n=9) of the population with other diagnosis than ARFID, followed by Sensory Processing Disorder plus Global Developmental Delay diagnosed in 15.8% (n=6) and Autism Spectrum Disorder plus Intellectual Developmental Disorder (10.5%, n=4).
Discussion/Conclusions: Our results underscore the neurodevelopment comorbidity among individuals with ARFID, and highlight the potential shared psychopathology between specific ARFID characteristics and other psychiatric dimensions that may be relevant for treatment.
An unusual case of Isolated piemeningeal hemangioma in a child with significantly elevated cerebrospinal fluid protein in Sturge–Weber syndrome (Type III)
Authors: Zhu Jicai , Hu Yulei , Liu Ailin , Su Lina , Guo Cheng , Ma Mingmin , Xiong Caiyun , Ju Yan , Chen Xiaofang , Tang Chunhui
Published: October 2023

Background: Sturge-weber syndrome is a rare congenital neurocutaneous disorder. Its main feature is facial and meningeal hemangioma. Sturge-weber syndrome may present pial meningeal hemangioma but no facial hemangioma in a small number of patients. However, in children with Sturge-Weber syndrome disease, only cerebral hemangioma, but no facial hemangioma, and elevated cerebrospinal fluid protein level have not been reported.
Case presentation: A Chinese male children presented with leptomeningeal angiomatosis,seizure, the protein level of cerebrospinal fluid increased significantly and no facial hemangioma and ophthalmic symptom. The imaging examination were consistent with the diagnostic criteria of Sturge-Weber syndrome.
Conclusions: We report a case of Sturge-Weber syndrome with cerebral hemangioma but no facial hemangioma and a significant increase in cerebrospinal fluid protein.
An Innovative Approach for Text Document Identification through Word Clouds technique
Authors: Khandagale Sachin , Kurundkar Gajanan , Tathe Rajkumar , Kawade Dipak
Published: October 2023

Text is a versatile and valuable approach to conveying information and knowledge. Unstructured data, however, poses challenges when it comes to reading and extracting valuable information. Users cannot find their subject domains within a matter of seconds without reading them at all. To accurately determine the category of a document or text data, the user must identify the presence of certain key sections in the document. So, text analysis is required to get hidden features from data. Word cloud is one of the important tools in machine learning useful for summarizing and visualizing important words and themes. It can extract relevant features necessary for categorizing text documents into appropriate themes. In this study, we use a document identification approach based on word clouds. Present study uses various news for identification purpose which were downloaded from different newspaper websites. People can find their topics in seconds without even reading them. This study provides numerous interactive graphics and robust, customizable features to help understand the subject of a document.
Achieving the Automation of Knee Joint Functional Scoring Through Based on Improved YOLOX
Authors: Yang Tao , Zhao Jie , Wang Ben , Wang Li , Bao Hengzhe , Li Bing , Luo Wen , Zhao Huiwen , Liu Jun
Published: October 2023

Objective
This study aims to assess the feasibility of computer model-based evaluation of knee joint functional capacity in comparison to manual assessments.
Methods
The study consists of two phases: (1) developing the automatic knee joint action recognition and classification systems based on improved YOLOX; (2) analyzing the feasibility between the software systems and doctors, identifying knee joint function of patients, and determining the accuracy of the software systems.
Results
In this study, the items of knee joint assessment included the stair climbing, walking on uneven surfaces, and knee joint function in the life. The weighted Kappa coefficients between doctors and the software systems are 0.76180, 0.64148, 0.68792, respectively, indicating good consistency of software systems.
Conclusion
This paper introduces an automatic knee joint action recognition and classification method based on improved YOLOX. Comparing the results between the orthopedic doctors and software system, the effectiveness of this software system is validated.
Patterns of wild edible plants consumption of District Swat, Pakistan: Emphasizing the importance of plants in uplifting the quality of life of local residents
Authors: Ali Shujat , Qurashi Rahmat Ullah , Munazir Mehmooda , Shah Sayed Afzal , Hussain Wahid , Bussmann Rainer W.
Published: October 2023

The people of Swat possess vast knowledge of wild food plants and used as dietary component. There is dire need to document such knowledge because these plants can serve as alternate source of energy, nutrients, minerals, and phytochemicals in a scenario of global food shortages and ever-increasing health problems. The present research is an endeavor to document such data from the Swat district, Pakistan. For this purpose field surveys were carried out during 2018 to 2022 and various growing localities of the region were visited for data collection from knowledgeable locals using semi-structured questionnaire, oral discussions and personal observations. In addition, the available literature on the area 26 was searched to identify novel food plant species. In all, a total 175 plant species belonging to 64 families were identified. The family Rosaceae contributed maximum plant species (19), followed by Fabaceae (15 spp.), Brassicaceae (12 spp.), 28 Rhamnaceae (9 spp.), Lamiaceae (8 spp.), Asteraceae, and Solanaceae (6 spp. each), Polygonaceae and Moraceae (5 spp. each). Fruit was the most widely used part (43%), followed by the shoot (39%), leaves (13%), seeds (10%), pods (5%), and miscellaneous parts (5%). Most of the plants were eaten as fruit (39%), followed by vegetable (37%); while 33% were used for making sauces, curries, flavoring agents, salads, or eaten in raw form. The current study reflected the role of plants as life-sustainers and life-savers for local communities. This research work is expected to provide basis for future research in nutrition science.
The expression of deiodinase 2 and deiodinase 3 are reduced by insulin and D-glucose respectively, in human placental explant cultures
Authors: Herrera-Chavez Nestor , Appel-Rubio Javiera , Rojas Daniela , Mennickent Daniela , Buchert Jorge , Roble Katherine , Sanchez Andrea , Aguayo Claudio , Gonzalez Marcelo , Guzmán-Gutiérrez Enrique
Published: October 2023

Introduction: Gestational diabetes mellitus is associated with altered expression of deiodinases (DIO), a group of seleno-enzymes that metabolize thyroid hormones in several tissues, including human placenta. It has been reported that these alterations could lead to reduced fetal thyroid hormone levels and impaired central nervous system development. However, it is not clear if D-glucose or insulin, which levels are increased in metabolic pathologies such as Gestational diabetes mellitus, are responsible for this phenomenon.
Methods: We recruited 10 women with normal pregnancies from Hospital Guillermo Grant Benavente, Concepcion, Chile. After delivery, explants were extracted from placenta to perform cultures exposed to different concentrations of D-glucose and insulin, in order to evaluate deiodinase mRNA expression by RT-qPCR, enzymatic activity and protein localization by immunohistochemistry.
Results: We observed that insulin could decrease both DIO2 mRNA (~38%) and activity (~40%), and D-glucose diminished DIO3 mRNA (~48%) as well as its activity (~36%). At control conditions DIO2 expression was observed mainly in fetal vasculature, while DIO3 expression focused on macrophage-like cells. D-glucose did not change deiodinase localization in placenta, whereas insulin promoted DIO2 and DIO3 expression in syncytiotrophoblast.
Conclusion: We suggest that a diabetogenic state in pregnancy with high levels of D-glucose and insulin, may lead to deiodinase alterations in placenta and consequently fetal thyroid dysfunction.
WITHDRAWN: Descriptive epidemiology of 30,223 histopathologically confirmed meningiomas in France: 2006–2015
Authors: Depond Charles Champeaux , Zouaoui Sonia , Darlix Amélie , Rigau Valérie , Mathieu-Daudé Hélène , Bauchet Fabienne , Khettab Mohamed , Trétarre Brigitte , Figarella-Branger Dominique , Taillandier Luc , Boetto Julien , Pallud Johan , Zemmoura Ilyess , Roche Pierre-Hugues , Bauchet Luc
Published: October 2023

Purpose
To describe the epidemiology of meningioma operated in France between 2006 and 2015.
Methods
We processed the French Brain Tumour Database (FBTDB) to conduct a nationwide population-based study of all histopathologically confirmed meningioma in France. Descriptive methods were implemented.
Results
30,223 meningiomas cases were operated on 28,424 patients, in 61 centres. Meningioma was 3 times more common in women (74.1% vs. 25.9%). Mean age at surgery was 58.5±13.9 years; 57.9±13.5 years for women vs.59.9 ±14.8 years for men (p<0.001). The incidence of meningioma surgery increased with age. Grade 1, 2, and 3 meningiomas accounted for 83.9%, 13.91% and, 2.19% respectively. The proportion of grade 2 significantly grew over the study period (p<0.001). There was a significant variability of meningioma grading by institutions, especially for grade 2 which spanned from 5.1% up to 22.4% (p <0.001). 89.05% of the patients had solely one meningioma surgery, 8.52% two and, 2.43% three or more. The number of surgeries was associated with the grade of malignancy (p <0.001).
Conclusion
The FBTBD is an effective source that comforts previous studies and, bring new information from 30,223 meningiomas cases operated on over a 10-year-long period.
Three quarters of meningioma were removed in women. The incidence of meningioma surgery increased with age and, peaked at 58.5 years. They were predominantly benign with meningothelial subtype being the most common. However, the rate of grade 2 has significantly grown over the time, on contrary to malignant meningioma proportion, which remained rare and stable over time around 2%.
Prognostic Value of HIF1A-AS3 in Breast cancer
Authors: Yong Liyun , Luis Manuel A , Yin XiaoXing , Cai Lu , Dique Marcia R , Tang Shasha , Cai Fengfeng
Published: October 2023

Breast cancer (BC) is the predominant malignant tumor among women globally. It has high recurrence which have adverse effects on patient outcomes. Therefore, there is need to identify effective biomarkers for prognostic evaluation. Numerous long non-coding RNAs (lncRNAs) have been shown to be implicated in the regulation of different stages of BC tumorigenesis. The role of lncRNA hypoxia-inducible factor 1 alpha-antisense RNA 3 (HIF1A-AS3) in BC has not been studied. The purpose of this study was to find a novel biomarker that can guide the formulation of effective treatments. The relationship between HIF1A-AS3 and BC was explored using the Breast Cancer on The Cancer Genome Atlas (TCGA-BRCA) database. The expression level of HIF1A-AS3 in various BC cells and normal breast cell, BC and paracancerous tissues from clinical patient samples, as well as primary breast cancer cell, respectively was analyzed. The R package was used to analyze RNA sequencing data and identify the differentially expressed genes (DEGs) between BC tissues with high and low expression of HIF1A-AS3 from the TCGA-BRCA database. Kaplan-Meier (KM) and Cox regression analyses were used in the current study to find the predictive value of HIF1A-AS3 on clinical outcomes in patients with BC. The association between infiltration of HIF1A-AS3 and immune cells was determined through Spearman's correlation analyses. The results revealed that the expression of HIF1A-AS3 was significantly elevated in breast cancer cell, BC tissues and primary breast cancer cell compared with that in normal breast cell, paracancerous tissues, and primary breast epithelial cell. KM analysis showed that high expression of HIF1A-AS3 was associated with poor OS [hazard ratio (HR): 1.42, P = 0.029], progression-free interval (PFS) (HR: 1.60, P = 0.005), and DSS (HR: 1.60, P = 0.031), especially in elderly patients below the age of 60, clinical T stage T1 and T2 as well as pathological stages III and IV. This suggested that higher HIF1A-AS3 expression levels were associated with unfavorable prognosis in BC. Moreover, analysis of immune infiltration showed that HIF1A-AS3 expression was associated with some types of immune infiltrating cells. The study identified that HIF1A-AS3 is a potential prognostic biomarker in BC.
Tertiary lymphoid structures critical for the prognosis of patients with cervical cancer
Authors: Zhang Qianqian , Wang Mengxin , Wu Suhui
Published: October 2023

Cervical cancer (CC) is the most common female reproductive system tumor. However, the tertiary lymphoid structures (TLSs) associated with CC have not been well studied. This study aimed to develop a novel TLS-based signature to stratify the prognosis of patients with CC and predict the clinical outcomes. The data were obtained from the TCGA (n = 292) and GEO (n = 300) databases. We classified TLS subtypes (C1 and C2) of CC based on the TLSs-9 gene signature using unsupervised hierarchical clustering, with the C1 subtype having a worse prognosis than the C2 subtype. Regarding immune-related features, immune and stromal scores, immune checkpoint gene expression, and tumor mutation burden were significantly higher in C2 than in C1. We found higher infiltration ratios of memory B cells, CD8+ T cells, activated memory CD4+ T cells, and activated macrophages in C2. Patients with C1 were more sensitive to cisplatin, doxorubicin, bleomycin, vinorelbine, and imatinib than those with C2. The risk score model and prognostic nomogram constructed based on the TLS signature were significantly and positively correlated with the survival rate of patients with CC. The presence of TLSs provides new insights into the prognosis of patients with CC, contributing to the precise stratification and accurate treatment.
Physicochemical Interaction of Microstructured Drumsticks withSynthetic Dye: The Tailoring of Surface Phenomenon in Technology
Authors: Rahman Md. Wasikur , Nipa Sumaya Tarannum , Shefa Nawrin Rahman , Akter Rumana , Khan Md. Maksudur Rahman , Uddin M. Jasim
Published: October 2023

The current study shows that drumstick powder (DSP) is a potential adsorbent to remove synthetic Congo Red (CR) from wastewater (WW) by a batch technique. CR as a textile waste harms the ecology and the water ecosystem, and it should be removed from WW. At various experimental conditions, various operating parameters such as initial concentration (IC) of dye (25–300 ppm), contact time (10–240 mins), pH (1–12) and adsorbent dosage (0.1-1.0 g/L) were investigated. The reaction kinetics and adsorption isotherms were examined. Maximum removal of dye has been found at pH 2. Dye concentration is an important factor, and it is inversely related to the percentage removal. 100 ppm was chosen as the IC for the optimization experiment. Increasing the amount of adsorbent dosage and contact time, there is a rise in the percentage removal of dye particles. The optimum adsorbent dosage was found 0.5 g/L and the contact time 90 mins. The maximum adsorption capacity (qmax) has been found to be 114.94 mg/g. The adsorption mechanism closely matched the Freundlich isotherm and pseudo-second order (PSO) kinetic models.
Modeling monthly streamflow of intermittent rivers in semi-arid regions using a hybrid forecasting model
Authors: Al-Juboori Anas
Published: October 2023

The process of forecasting the monthly flows of intermittent rivers is one of the important issues in hydrology as well as in managing water resources and designing hydraulic structures for those rivers. In this research, Support Vector Machine (SVM) model was used to predict the monthly flows of the Adhaim River in Iraq, and the results showed that the application of SVM model failed to model intermittent monthly flows, as the value of the determination coefficient did not exceed 0.23 in the validation stage of the model. A hybrid model consisting of three machine learning models, K-means clustering, Bayesian networks, and Support vector machine, was applied to predict intermittent monthly flows. The results proved the efficiency of the proposed hybrid model in predicting monthly streamflow. The value of the coefficient of determination was 0.84 at the model validation stage. The application of the proposed hybrid model improved the results by 72% compared to the implementation of the SVM model.
Frequency Stabilization of diode laser via Two-photon transitions spectroscopy induced by optical frequency comb pulses combined with diode laser
Authors: zhang yichi , han hongyan
Published: October 2023

We present the observation of high-precision direct frequency comb spectroscopy
excited by an optical frequency comb and a diode laser when each of them
drives one step of the two-photon transitions in a rubidium vapor system. We
demonstrate a stable and low noise system by directly locking the frequency of
continuous-wave laser to rubidium two-photon transitions. The frequency stabil
ity of diode laser via two-photon transitions locking technique is 8*10−11 for a
1-s gate time and 3*10−12 for 1000-s. It proves a potential technique to locking
the diode laser with high stability. We choice the suitable optical frequency comb
pulse and the frequency of diode laser to fulfill the double-resonance condition.
These techniques eliminate spectrum lines overlap would benefit spectroscopy
measurement.
Educational Interventions for Imposter phenomenon: a scoping review
Authors: Siddiqui Zaha Kamran , Church Helen , Jayasuriya Raveen , Boddice Timothy , Tomlinson James
Published: October 2023

Background
Imposter Phenomenon (IP) is a subjective feeling of intellectual fraud and self-doubt experienced by individuals in goal-orientated high-achieving professions, regardless of gender. The impact of IP within healthcare has been associated with individual physical and mental health and concerns around training, career progression and DEI at an institutional level. To effectively address IP in healthcare, this scoping review aims to explore educational interventions designed to empower high-achieving individuals with the tools needed to confront and overcome IP.
Methods
The scoping review adhered to a predetermined protocol informed by the JBI methodology and PRISMA-ScR guidelines in order to identify educational interventions addressing IP in high-achieving industries. Articles were searched across multiple databases, including MEDLINE (Ovid), PsychINFO, SCOPUS, and Web of Science, alongside grey literature, without imposing any time constraints.
Results
Seventeen articles were incorporated into the review, with the majority originating from the USA and majority being published since 2020. Ten studies targeted healthcare professionals, undergraduate and postgraduate healthcare students. Majority of studies aimed at addressing IP, featured a larger number of female participants than males. Workshops with self-reflection and group-guided exercises to overcome IP were the most popular educational interventions. Coaching and structured supervision were also suggested. Across all papers, three themes emerged for coping strategies: individual, peer-to-peer, and institutional.
Conclusions
This scoping review suggests how group and individual interventions such as workshops, small group discussions and coaching can be used to overcome IP in healthcare. Institutional changes like diversity promotion, supervisor education, and support networks are crucial in addressing IP. Further long term and speciality specific assessments are needed to measure impact. Overall, the review highlights how educational awareness and a variety of strategies can be implemented to create a supportive environment for professionals dealing with IP, promoting their well-being and success.
The long-term outcome and safety of severe aplastic anemia treated with porcine antilymphocyte globulin plus cyclosporine, with or without thrombopoietin receptor agonists: a double-center retrospective study
Authors: Liang xingquan , xu shan , xiao yue , Lu yan , deng mingyang
Published: October 2023

Objectives: To evaluate the long-term outcome and safety of porcine antilymphocyte globulin plus cyclosporine, with or without thrombopoietin receptor agonist (TPO-RA), for patients with severe aplastic anemia (SAA) and identification of factors influencing hematology response and long-term outcome.
Methods: We retrospectively analyzed the data of 85 people with newly diagnosed SAA who underwent porcine antithymocyte globulin (p-ATG) plus cyclosporine (CsA), with or without TPO-RA in the second Xiangya hospital and the first people’s hospital of Chenzhou during 2014 to 2023.
Results: The overall response rates were 55.3% and 65.9% with 3 and 6 months. Hematologic response rates for the TPO-RA group were 66.7% and 72.3%, at 3 and 6 months and without the TPO-RA group were 27.8% and 55.6%. In multivariate analysis, platelet count＞10x109/L was a predictor of favorable response at 6 months (P= 0.015). The median follow-up time for all patients was 39 months (range 0.4~104), the 5-year OS rate of 90.6% [95% CI = 82.1–95.2%], and the FFS rate was 68.9% [95% CI =56.6–78.4%]. The very severe aplastic anemia（VSAA）subgroup was an independent unfavorable predictor of failure-free survival (FFS) and overall survival (OS). 12 patients (14.1%) suffered from serum sickness, and 9.5% of patients had mild hepatic impairment.
Conclusions: p-ATG can be used as an appropriate alternative preparation for rabbit ATG, with fewer side effects and lower medical costs, and combined with TPO-RA may improve early hematologic response rates.
Experimental Study on Fatigue Mechanical Properties and Acoustic Emission Behavior of Hard Layer Sandstone under Cyclic Disturbance
Authors: Cheng Yun , Song Zhanping , Wu Fahong , Yuan Wei , Yang Tengtian
Published: October 2023

The fatigue deformation of sedimentary rock at tunnel bottom under the train disturbance load contains the mechanical damage behavior. The fatigue mechanics test and acoustic emission (AE) monitoring are carried out to investigate the fatigue deformation and AE response characteristics of layer sandstone under fatigue load. This study showed that there is a time-sensitive correspondence between hysteresis loop and layer degradation effect. The hysteresis loop undergoes intensive distribution, strain transition, sparse distribution and fracture instability, and the continuous stress drops induces a significant strain transition. The layer structure has a significant deterioration effect on bearing strength and fatigue life. The cycle peak strength is degraded by 15.05%-28.19% compared with static peak strength. The fatigue life is positively correlated with peak strength and fatigue life decreases first and then increases with the layer angle increasing. The fracture modes of layer sandstone has anisotropic properties, including tensile fracture I, tensile fracture Ⅱ, shear fracture and composite fracture, and the fractal dimension is negatively correlated with layer degradation effect. AE amplitudes at low-medium-high stress levels are distributed in banded shape, tower shape and semi-tower shape, showing a time-corresponding relationship with the stress paths. There is a competitive relationship between compaction strengthening and fracturing damage. The evolution stages of AE damage variable include damage calm phase, damage acceleration phase and damage burst phase, and 0.20 is the starting point of damage acceleration and mutation damage (DAE−C) near the peak stress indicates a complete fracture. The stronger the layer deterioration effect, the smaller the DAE−C. The result may be represents a useful complement to mechanical properties of sedimentary rocks and is of important for ensuring the rock engineering safety.
Preparation of Cu(І)Y from binary Cu(II) precursors and its CO adsorption performance
Authors: Zhang Jiali , Li Xue
Published: October 2023

Separation and purification of CO is an important chemical process. Adsorption is competitive due to its efficient operation, low energy and matured technology. For CO adsorption, the capacity and selectivity of the adsorbent is the key factor to determine the efficiency. In this paper, C2H5OH was used as the dispersing agent, CuCl2 and Cu(CH3COO)2 were used as the precursors mixing with HY molecular sieve, then followed by activation at different temperatures to prepare CO adsorbents. The maximum CO adsorption capacity was 42 mL/gHY, which was 28.4 % higher than that of the adsorbent prepared with water as the dispersing agent. The adsorbents were characterized by BET, XRD, XRF, XPS and TG-MS. It was revealed that Cu(II) can be reduced to Cu(I) at 290℃ and highly dispersed on surface of HY. Ion exchange occurred between HY molecular sieve and Cu((I) salts, and Cu (I) Y came into being. Compared with Cu(II), Cu and even CuCl/HY, Cu (I) Y had the best CO adsorption performance.