bluuebunny/crossref_metadata_2025 huggingface dataset:
PSVI-18 Efficiency of the use of ultrafine particles of silicon dioxide in the synchronization scheme of estrus of heifers
Authors: Platonov Stanislav , Khristianovsky Pavel , Frolov Alexey , Zavyalov Oleg
Published: October 2021

The intensification of the reproduction of a herd of cattle requires the use of highly effective synchronization schemes for estrus. All schemes available today give a low percentage of fertilization with frontal insemination. The aim of this study was to assess the effect of ultrafine particles of silicon dioxide on fertility and the level of sex hormones in heifers during synchronization of sexual heat with subsequent frontal insemination. Ultrafine particles of silicon dioxide were injected in isotonic sodium chloride solution. The synchronization scheme included two-fold application of prostaglandin preparations; blood was taken from under the tail vein to determine the hormonal status on days 1, 11, and 14 of the experiment. The results of the study showed that ultrafine particles of silicon dioxide had a positive effect on the level of sex hormones. Concerning progesterone, the difference between the values of increase in the level in the experimental and control groups was 112.1% (P ≤ 0.01) on the 11th day. On the 14th day of the experiment, the difference between the values of the decrease level was 19.6% (P ≤ 0.05). Follicle-stimulating hormone increased by 9.1% (P ≤ 0.05) by the 14th day of the experiment compared with the previous value. In the heifers of the experimental group, the excess was more significant, i.e.15.4% (P ≤ 0.05); the level of luteinizing hormone in the experimental group on the 14th day of the experiment exceeded the level of the control by 31.1% (P ≤ 0.05). According to the results of the examination for pregnancy, an increase in fertility was revealed by 20.0% in the experimental group in comparison with the control group. This study demonstrates the potential of using ultrafine silica particles in estrus synchronization schemes for heifers to increase heifer fertility. The research was carried out in accordance with the research plan for 2019–2021. FGBNU FSC BST RAS (No. 0761-2019-0006).
PSVI-15 Combined effect of C73T × C528T polymorphisms in the leptin gene with quantitative and qualitative indicators of meat productivity
Authors: Lapshina Alexandra , Kolpakov Vladimir , Dzhulamanov Kinispay , Gerasimov Nikolay , Dubovskova Marina
Published: October 2021

The LEP is a highly polymorphicgene and it is associated with genetic differences in various productivetraits in cattle. The aim of research was to study the combined effect of C73T and C528T polymorphisms in leptin gene on meat productivity and carcass grade in Aberdeen-Angus cows and heifers. Heifers (n = 49) were slaughtered at the age of 20 months and cows (n = 30) after the first calving (3 years). There were seven haplotypes C73T (GenBank AF120500) × C528T (GenBank AB070368) identified in cows with different frequency. The maximum frequency was established in combined genotypes CT/CT (0.37), TT/CC (0.27) and CT/CC (0.13). The most massive carcasses (337.5 kg) were obtained from cows-carriers of the CT/CC haplotype, which exceeded their peers by 14.8–24.7 kg (4.59–7.90%; P >0.05). However, cows carrying the CT/CC genotype had a minimum carcass yield of 49.8%. There were eight haplotypes identified in heifers. The highest frequency was in combinations CT/CT (0.26), TT/CC (0.20), CC/TT (0.20), CC/CT (0.12), TT/CT (0.10). Heifers carrying the TT/CT genotype were characterized by the highest live weight (568.9 kg) and carcass weight (331.2 kg), exceeding the indicators of peers by 8.9–43.5 kg (1.59–8.28%; P < 0.05, P > 0.05) and 10.9–22.2 kg (3.40–7.18%; P < 0.05, P > 0.05), respectively. The maximum carcass yield was recorded in carriers of the CT/CC haplotype (60.7%). The best carcasses grade were observedin heifers with a combination of CC/TT and CT/CT genotypes. The highest categories (Prime and TopChoice) were assigned to 70.0 and 69.2% of carcasses, respectively. The superiority of haplotype CT/CT in the quality of meat raw materials was confirmed in cows. Thus, the combination of C73T and C528T polymorphisms in the leptin gene is associated with intra-breed variability in live weight, carcass weight and yield, and carcass grade in Aberdeen-Angus cattle. This research was performed with financial support from the project 0526-2021-0001 of RAS.
PSVII-18 Change in the taxonomic composition of ruminal microflora against the background of copper and plant extract
Authors: Atlanderova Kseniya , Makaeva Ayna , Miroshnikov Sergej , Duskaev Galimzhan
Published: October 2021

Plant extracts are used in cattle diets to stimulate growth and immunity, while the unstable biochemical composition of plants contributes to the search for effective compositions that exhibit a synergistic effect. Objective of the study is the effect of an aqueous extract of oak bark (0.7 ml/kg of live weight) - group I, copper particles specific surface area 24 m2/g; 0.06 mg/kg of live weight - group II, their compositions (group III) on the taxonomic composition of the bacterial community of the rumen of cattle. Bulls had rumen fistulas Bos taurus at the age of 13 months (n = 5). Test samples of feed components were given with a concentrated portion of the main diet. Diet contain 60% - roughage (hay of Sudanese grass and alfalfa), 35% - concentrated feed (mixture of barley, wheat, sunflower husk, bran). The taxonomic composition of ruminal content was determined by NGS sequencing on a MiSeq device (Illumina, USA). It was found that the combined use of the extract and copper UFPs increases the concentration of representatives of Bacteroidetes in the rumen by 5.6%, the separate use of copper UFPs is by 20.7% (P ≤ 0.05), at the same time the values of representatives of Firmicutes decreased - by 7.2 and 24.7% (P ≤ 0.05) in comparison with the control. At the taxon level, the following classes prevailed: Bacilli (2.6%) in group I; Bacteroidia (17.0%) in II, in III - Bacteroidia (5.0%) in comparison with the control. In the experimental groups, at the genus level, a lower content of Streptococcus and the absence of Faecalibacterium were registered. Thus, the composition of plant extract and copper has an inhibitory effect on certain representatives of pathogenic bacteria. The results obtained indicate that the combined use of these substances is promising. This research was performed with financial support from the RSF (20-16-00088).
PSVIII-7 Genetic parameters for health traits in dairy calves
Authors: Lynch Colin , Oliviera Hinayah , van Staaveren Nienke , Miglior Filippo , Schenkel Flavio S , Baes Christine F
Published: October 2021

Recent issues in the dairy industry related to both animal and public health concerns are leading farmers away from the use of drugs, while placing more focus on animal health and welfare. Public demands are also shifting towards ensuring socially acceptable production practices in terms of good animal health and welfare. Such challenges are moving the focus in dairying from solely financial to a broader set of themes that, once addressed, will enhance the sustainability of dairying and provide a long-term competitive advantage for the Canadian industry. Furthermore, from a production standpoint, calf diseases, such as, diarrhea and respiratory disease (RD) have been associated with decreased first lactation production and growth rate, therefore decreasing an animal’s potential lifetime profitability. As part of a larger project aiming to add calf health traits to genetic evaluations in Canadian dairy cattle, this study provides the groundwork through the estimation of genetic parameters of two calf health traits, diarrhea and RD. Data were provided by Lactanet Canada, and included 20,594 calf records for diarrhea from 741 herds, and 48,927 calf records for RD from 1,412 herds, recorded between 2004 and 2021 across Canada. Total herd records ranged between 1 and 3,860 for RD with an average of 37 records per herd, while for diarrhea records ranged between 1 and 3,724 with an average of 28 records per herd. The results of this study will be used to optimally fit both diarrhea resistance and RD resistance into a novel resiliency index for use in national genetic evaluations in Canada.
PSVIII-4 Genetic evaluation of functional heifer longevity in north American angus cattle
Authors: Oliveira Hinayah R , Miller Stephen P , Brito Luiz F , Schenkel Flavio S
Published: October 2021

The goals of this study were to develop a genetic evaluation system for a novel trait called functional heifer longevity (FHL), and determine if this novel trait is heritable. The FHL trait was defined as binary, in which the heifers received the code 1 if they had calved by the end of their third year (n = 377,938), or 0 if they were culled/sold during this period (n = 368,308). Analysis were performed using linear animal models and Bayesian inference. The significant systematic effects included in the statistical models are born by embryo transfer, year-season of birth, and age at calving (in months). Three models, differing according to their random effects (i.e., reduced model, which included only herd-year-season and additive genetic random effects; maternal genetic model, which added maternal genetic effects; and complete model, which further added maternal permanent environmental effects), were compared based on the deviance information criterion (DIC) and the estimates of genetic parameters. The reduced model was preferred according to the DIC values. However, high maternal heritabilities were estimated using the maternal genetic (0.51) and complete (0.36) models, indicating that maternal effects can impact the selection of heifers for breeding. Similar additive genetic heritabilities were estimated among the three models (0.24, 0.27, and 0.25 using the reduced, maternal genetic, and complete models, respectively), and no significant re-ranking of selection candidates were observed based on their additive genetic breeding values. Total heritabilities and correlations estimated between additive genetic and maternal genetic effects were 0.37 and -0.28 for the maternal genetic, and 0.31 and -0.27 for the complete model, respectively. This study shows that FHL is heritable, and that including maternal effects in the statistical models might be important. These results contribute to a larger project studying the genetics of female longevity in Angus cattle.
PSVIII-6 Genetic features of Russian Simmental cattle population different origin derived by assessing milk production and fertility traits
Authors: Ignatieva Larisa P , Sermyagin Alexander A , Nikitin Sergey
Published: October 2021

Since the beginning of the 20th century, over 43 thousand animals of Simmental cattle and related breeds have been imported to Russia from Europe. This had a significant impact on the selection intensity and genetic structure within the pale-motley population. The research included 42 breeding herds from 14 regions of Russia. The EBV using the BLUPF90 program based on the ANIMAL MODEL procedure was calculated. Totally of 1471 bulls by using 61816 daughters were estimated. The heritability coefficients showed moderate variability for milk yield (MY) h2=0.140, fat percentage (FP) h2=0.194, protein percentage (PP) h2=0.184 and live weight h2=0.163, as an indirect predictor for meat production. The variability for fertility features like number of inseminations per conception and days open was h2=0.073 and h2=0.061, respectively. Genetic correlation between MY and FP was rg=0.149, and for MY and PP – rg=0.180, while between FP and PP – rg=0.226. The average bulls’ EBV for milk traits in Red Holsteins consisted +23 kg MY, +0.002% FP and +0.001% PP, by Austrian Simmental +10 kg MY, +0.013% FP and +0.002% PP. For Russian Simmental origin animals was signed negative selection response for MY (-23 kg), FP (-0.001%) and PP (-0.003%). High average EBV for imported Simmental cows were revealed +146 kg MY, +0.009% FP and +0.004% PP, while in Russian Simmental we got negative values for MY -70 kg and PP -0.004% but only for FP was showed positive level +0.001%. Simmental breed improvement strategy in Russia focused not only to increasing milk yield but also to improving milk compositions with stabilizing cows’ reproductive traits using the world gene pool of related breeds from Europe. The study was funded by Ministry of Science and Higher Education No.0445-2021-0016
PSVIII-9 Genome assembly of American mink (Neovison vison) using high-fidelity long reads
Authors: Karimi Karim , Do Duy Ngoc , Miar Younes
Published: October 2021

Development of genome-enabled selection and providing new insights in the genetic architecture of economically important traits are essential parts of mink breeding programs. Availability of a contagious genome assembly would guarantee the fundamental genomic studies in American mink (Neovison vison). Advances in long-read sequencing technologies have provided the opportunity to obtain high quality and free-gaps assemblies for different species. The objective of this study was to generate an accurate genome assembly using Single Molecule High-Fidelity (HiFi) Sequencing for American mink. The whole genome sequences of 100 mink were analyzed to select the most homozygous individual. A black American mink from Millbank Fur Farm (Rockwood, ON, Canada) was selected for PacBio sequencing. The total number of 2,884,047 HiFi reads with the average size of 20 kb were generated using three libraries of PacBio Sequel II System. Three de novo assemblers including wtdbg, Flye and IPA were used to obtain the initial draft of assembly using the long reads. The draft generated using Flye was selected as the final assembly based on the metrics of contiguity and completeness. The final assembly included 3,529 contigs with the N50 of 18.26 Mb and the largest contig of 62.16 Mb. The length of genome assembly was 2.66 Gb with 85 gaps. These results confirmed that high-coverage and accurate long-reads significantly improved the American mink genome assembly and successfully generated more contagious assembly. The chromosome conformation capture data will be integrated to the current draft to obtain a chromosome-level genome assembly for American mink at the next step of the project.
PSVIII-8 Genetic parameters for parasite resistance in an endangered and heritage sheep breed from Florida
Authors: Estrada-Reyes Zaira M , Hidalgo Moreno Jorge A , Diehl Brittany N , Ogunade Ibukun M , Pech-Cervantes Andres A , Terrill Thomas H , Rae Owen
Published: October 2021

The Florida Native Sheep is one of the oldest sheep breeds in the United States. This heritage breed from Florida, naturally adapted to humid and hot climate conditions, is one of the most parasite resistant breeds from the Southern US. However, only approximately 1,000 individuals remain alive in the world. Therefore, conservation efforts and breeding programs are critical for survival of this breed. The objective of this research was to estimate genetic parameters for parasite resistance and body condition score in Florida Native sheep. The pedigree file contained 695 animals born between 2018 and 2020 and included 279 individuals with genotypes (38,429 SNP after quality control). The dataset contained 365 animals with phenotypic records at 38 days post-infection (natural Haemonchus contortus infection) for fecal egg count (FEC), blood packed count volume (PCV), FAMACHA score (FAM), and body condition score (BCS). Genetic parameters were estimated using a multi-trait model with a Bayesian implementation in the GIBBS3F90 program. Heritabilities were 0.38 0.07, 0.47 0.05, 0.27 0.04, and 0.52 0.07 for FEC, PCV, FAM, and BCS. Genetic correlations among parasite resistance traits were high and favorable: -0.82 0.06 (FEC-PCV), 0.83 0.07 (FEC-FAM), and -0.94 0.03 (PCV-FAM). Genetic correlations among parasite resistance traits and BCS were -0.42 0.11 (FEC-BCS), 0.75 0.09 (PCV-BCS), and -0.82 0.05 (FAM-BCS). Genetic progress for parasite resistance is possible in Florida Native sheep. The FAMACHA score is a phenotypic parameter easy to record in sheep; therefore, genetic selection for this trait can be effective to improve the remaining traits.
PSVIII-2 Genetic diversity analysis of hybrid Karachaev goats (Capra hircus) population using microsatellite marker data
Authors: Fomenko Oleg , Petrov Sergey N
Published: October 2021

The Karachaev goat is an indigenous breed that possesses unique features including significantly less fat deposition in the body compared to sheep and cattle, ability to graze at an altitude of up to 1200 meters and to produce fertile hybrids with wild relatives. To understand the genetic diversity and population structure of hybrids between domestic Karachaev goats (Capra hircus) and The West Caucasian tur (Capra caucasica) 143 individuals were analyzed using 10 microsatellites panel. Sample were analyzed according to the manufacturer’s recommendations on an automatic sequencer, ABI 3130XL genetic analyzer (Applied Biosystems). Genetic diversity was calculated using GenAlEx 6.503 software. Genotyping of ten microsatellite loci in hybrid forms of Karachaev goats and turs detected 106 alleles in total. Na values ranged from five (INR063) to seventeen (SRCRSO008), averaging 10.6 alleles per locus across the 10 loci. All loci were polymorphic. The average number of alleles was 10.6 alleles per locus. This is higher than the similar indicator obtained by Kharzinova et al. (2019) in populations of Soviet wool, Tajik wool, Orenburg downy, Alpine and Zaanen dairy breeds of goats, studied using the panel of same 10 STR-markers (9.3 loci). Other key indexes of genetic diversity could be found in table 1. The values of the coefficient FIS suggest the absence of related mating in the herd. Information on genotypic variability of Karachaev goats hybrid forms obtained here will contribute into the breeding programs improvement and to preservation of existing native breeds.
PSXI-7 Genomic analysis of heterosis in a composite beef cattle breed
Authors: Hay El Hamidi , Roberts Andrew
Published: October 2021

Crossbreeding is widely used in the beef cattle industry to exploit benefits of heterosis. This study evaluated the effects of heterozygosity on growth traits in an Angus x Hereford cross population. Moreover, a genome wide association study was conducted to detect regions in the genome with significant dominance effects on growth traits contributing to heterosis. A total of 1,530 animals, comprised of pure Line 1 Hereford, Angus and Angus x Line 1 Hereford crosses, were evaluated. Phenotypes included birth weight, weaning weight and yearling weight. All animals were genotyped with GeneSeek GGP LD 50k. Effects of genomic heterozygosity on growth traits were estimated. These effects were -0.76 kg (P < 0.001), 4.67 kg (P < 0.0001), 42.39 kg (P < 0.02) on birth weight, weaning weight and yearling weight respectively. A genome wide association study revealed several SNP markers with significant heterotic effects associated with birth weight, weaning weight and yearling weight. These SNP markers were located on chromosomes 1, 2, 14, 19, 13 and 12. Genes in these regions were reported to be involved in growth and other important physiological mechanisms. Our study revealed several regions associated with dominance effects and contributing to heterosis. These results could be beneficial in optimizing crossbreeding.
PSXI-8 Heritability estimates of antibody- and cell-mediated immune response in north American angus beef cattle
Authors: Beard Shannon , Hodgins Doug C , Schmied Julie , Caswell Jeff , Lohuis Michael M , Miller Steve , Devani Kajal , Schenkel Flavio S , Mallard Bonnie
Published: October 2021

Including immune response traits in breeding programs has been suggested as a solution to improve overall animal health and enhance disease resistance but is not yet available for Angus cattle in North America. One way to measure immune response is to use the High Immune Response (HIR™) technology, which identifies animals with superior heritable immunity by evaluating antibody-mediated (AMIR) and cell-mediated (CMIR) immune response, allowing for the calculation of an estimated breeding value for total adaptive immune response for each animal. Before HIR™ can be included in beef cattle breeding programs, genetic parameters, including heritability, must be estimated for the traits involved. The objective of this study was to estimate a preliminary heritability for AMIR and CMIR in North American Angus beef cattle. On day 0 of the phenotyping protocol, cattle were immunized intramuscularly with a preparation of type 1 (CMIR) and type 2 (AMIR) antigens with adjuvant. On day 14, cattle received an intradermal injection of 0.1 mL of the CMIR test antigen in the right tail fold, and 0.1 mL PBS in the left tail fold. Change in cutaneous double skinfold thickness after 24 hours was used to assess CMIR. Blood was collected on days 0 and 14 to evaluate serum antibody to the type 2 antigen by ELISA to quantify AMIR. Heritability estimates were calculated for Canada and the USA separately, and then for both countries combined using single-trait animal models in ASReml and are presented in Table 1. The results of this study indicate that the heritabilities of AMIR and CMIR are moderate and emphasize the potential for its inclusion into Angus breeding schemes.
PSXI-6 Genome-wide SNP analysis of three Azerbaijani sheep breeds
Authors: Dotsev Arsen V , Deniskova Tatiana E , Bagirov Vugar A , Abilov Ahmed I , Reyer Henry , Wimmers Klaus , Brem Gottfried , Zinovieva Natalia A
Published: October 2021

Azerbaijani sheep breeds are of particular interest since this region is in close proximity to the Fertile Crescent – the proposed place of domestication. Molecular genetic studies of local breeds will help shed light on the history of sheep breeding. Our work was aimed to identify genetic characteristics of three sheep breeds from Azerbaijan: Bozakh (n = 19), Karabakh (n = 16) and Mazekh (n = 19). For genotyping, we used Illumina OvineHD BeadChip (AgResearch) containing around 600 thousand SNPs. PLINK 1.9 was used to perform quality control. Genetic diversity parameters and pairwise FST distances were calculated in R package diveRsity. After all quality control procedures, including LD pruning, 286663 SNPs were selected for subsequent analyses. PCA revealed that, while all the breeds formed their own clusters, only 1.62% of genetic variability was explained by the first component (PC1). Cluster analysis conducted in Admixture 1.3 showed that Bozakh was derived from Karabakh and Mazekh breeds. However, based on CV error, the number of clusters (K) judged to be one. Pairwise FST distances were quite low, with highest value of 0.017 between Karabakh and Mazekh and lowest (0.006) between Mazekh and Bozakh breeds. The global fixation index (FST) was 0.01, which indicated that 99% of genetic variability was due to within breeds differences. Expected heterozygosity was similar in Bozah and Mazeh (0.363±0.000 and 0.360±0.000, respectively) and a slight lower in Karabakh breed (0.353±0.000). We observed neither heterozygotes excess nor deficiency in all of the studied breeds. The inbreeding coefficient (Fis), ranged from 0.001 in Karabakh to 0.011 in Mazekh sheep. Our results demonstrate that the studied Azerbaijani sheep breeds are genetically close, but carry specific genomic components that allows consider them as valuable genetic resources. The study was funded by the RSF 21-66-00007 (genotyping) and the Russian Ministry of Science and Higher Education 0445-2019-0024 (expedition studies).
PSXI-3 Genome-wide association study for MIR-predicted milk fatty acids composition in Russian Holstein cattle population
Authors: Sermyagin Alexander A , Lashneva Irina , Ignatieva Larisa P , Kositsin Alexander , Gladyr Elena , Ermilov Alexander , Yanchukov Ivan , Zinovieva Natalia A
Published: October 2021

Milk fatty acids (FA) derived from infrared spectra are a new type of traits that allow fast and predictability use their in dairy breeding and herd cattle management. The Holstein animals feature are the high milk yield, but milk composition traits can be different according to population or country origin. The purpose of our study was to find out genetic variation for milk FA and detect QTLs associated with Holstein sires’ EBV in Russian cattle population. For this analysis, we used an experimental dataset for 36982 milk test records from 14 breeding herds in the Moscow region. Individual milk samples per animal analyzed routinely by MilkoScan7 for different FA types: C14:0, C16:0, C18:0, C18:1, saturated, mono- and polyunsaturated, short-, medium- and long-chain. Total number of bulls consisted 778 individuals including 222 animals with genotypes (39051 SNP, Illumina 50K). For calculating EBV by Sire Model, we applied BLUPF90. Plink 1.90 performed quality check control and GWAS procedure. Heritability coefficients were 0.071–0.155 for C14:0-C18:0 levels, 0.196 for C18:1, 0.083 for SFA, 0.018 for PUFA, 0.176 for MUFA, 0.114–0.155 for SCFA-LCFA levels. GWAS revealed most significant (P < 0.001-0.00001) frequently QTLs associated with FA content that were cited in articles previously for BTA5 (CHST11,C18:1), BTA6 (KCNIP4,C18:1; PPRAGC1A,C18:0), BTA11 (NRXN1,LPIN1,C18:1; NBAS,C18:0), BTA26 (PCDH15,PUFA; PRKG1,C18:1). These genes were responsible for synthesis milk fat, fertility, udder conformation traits, lauric, myristic, myristoleic, palmatoleic, oleic and other types of FA. In addition, we identified several QTLs for C14:0, C16:0, SFA, MUFA, SCFA, LCFA on BTA1 (137.32 Mb), BTA10 (5.50 Mb, 9.79 Mb), BTA14 (44.35 Mb), BTA19 (17.57–17.89 Mb) and BTA22 (14.02–14.06 Mb, 20.29–20.45 Mb). Our results are the first steps toward to understanding genetic and genomic mechanisms for using FA in selection processes to improve milk quality for Holstein cattle in Russia. The study was funded by RSF (project No. 21-76-20046)
PSXI-2 Genome-Wide association study and possibilities for genomic selection of Simmental cattle breed in Russia
Authors: Ignatieva Larisa P , Sermyagin Alexander A , Nikitin Sergey , Conte Alexander , Naryshkina Elena , Zinovieva Natalia A
Published: October 2021

The implementation of genomic selection methods in dairy cattle is limited due to the size of the reference population, quantitative traits variability and genetic structure of population. The Simmental cattle and related breeds in Russia occupy the third place by registered cows - 87.6 thousand, and are bred from the western borders of central Russia to Yakutia in Siberia. The study aim was to search for QTL using as a pseudo phenotype sires’ EBV to validate the effectiveness of genomic assessments for Simmental cattle in Russia. Reference group based on 358 bulls from different Russian regional populations was formed. By the dataset of 61976 cows, using BLUP Animal Model approach, animals’ EBV were calculated. The GBLUP procedure was used to get genomic prediction (DGV). Genotype quality control selected of 37143 SNPs. GWAS analysis was perform by Plink 1.90. To prove DGV we used verification procedure to comparison with EBV in training population dataset that showed slightly moderate repeatability for 305-milk yield - 37.6%, milk fat - 39.8% and milk protein - 43.6%. Significant SNPs associated with bulls EBV according to milk production and fertility traits revealed for: 305-milk yield on BTA5 (ACO2,p=0.0005), BTA29 (NAP1L4, P = 0.00004; KCNQ1, P = 0.00004); fat percentage on BTA3 (ROR1, P = 0.0003), BTA14 (CRH, P = 0.0003; TRIM55, P = 0.0003; DNAJC5B, P = 0.0003; REX2, P = 0.0020; ZFHX4, P = 0.0020) BTA17 (TLR2, P = 0.0011); days open on BTA1 (TNIK, P = 0.0007; FNDC3B, P = 0.0007), BTA21 (IGF1R, P = 0.0002). Using Cattle QTL Database, the identified genes were associated with milk yield, fat and protein percentage, protein yield, somatic cell score, protein-to-fat ratio, stearic fatty acid content, calving ease, daughter pregnancy rate, stillbirth and inseminations per conception. GWAS results identified significant SNPs associated with milk production and fertility traits that will allow in the nearest future improve reliability for genomic prediction of young bulls and boost genetic trend in the Russian Simmental cattle population. The study was funded by RFBR within project No.17-29-08030
PSXII-8 Mitochondrial DNA diversity within domestic reindeer in Russia
Authors: Kharzinova Veronika R , Dotsev Arsen V , Bardukov Nikolay V , Deniskova Tatiana E , Upadhyay Maulik , Krebs Stefan , Kunz Elisabeth , Medugorac Ivica , Zinovieva Natalia A
Published: October 2021

Domestic reindeer in Russia are a valuable resource of vital importance to the physical and cultural survival of the Northern indigenous minority. During the last decades, the mitochondrial (mt) genetic markers have been widely used as a molecular tool to investigate genetic structure and diversity of livestock species. Here we aimed at the assessing the mtDNA diversity of the domestic reindeer inhabiting the area from the Kola Peninsula in the west to the Chukotka region in the east. A complete cytochrome b (cytb) sequences (1,140 bp) from representatives of six populations, including Nenets (NEN, n = 16), Evenk (EVK, n = 12), Even (EVN, n = 6), Chukotka (CHU, n = 6), Chukotka-Khargin (CHUKH, n = 6) and Tuva (TUVA, n = 6) were obtained. Sequences’ alignment was conducted using MUSCLE algorithm in R package msa. In total, 34 haplotypes were identified. Median-joining network, constructed in PopART 1.7, revealed three major groups of haplotypes: the first one joined the samples of all the populations, the second one included NEN, EVN and CHUKH, and the third group was presented by the one sample of CHU. AMOVA, calculated in Arlequin 3.5.2.2, showed that only 9.58% of molecular variance could be explained by the differences between populations and 90.42% - within populations. Genetic diversity parameters calculated in DnaSP 6.12.03, demonstrated that average number of nucleotide differences (K) was highest in CHUKH (28.333) and EVN (27.409) and lowest in TUVA (4.533) and EVK (5.400). Nucleotide diversity (Pi) was 0.01238±0.00559, 0.00474±0.00091, 0.02404±0.00453, 0.01281±0.00464, 0.02485±0.00744, and 0.00398±0.00110 for NEN, EVK, EVN, CHU, CHUKH and TUVA, respectively. Our study demonstrated the lack of clear genetic structure of the studied reindeer populations in relation to cytb sequence. The level of genetic diversity was associated with census size and was lowest in the smallest Tuva population. This study was supported by RSF-21-16-00071 and Russian Ministry of Science and Higher Education-0445-2019-0024.
PSXII-1 Tracing the historical genetic components in turano-mongolian cattle breeds based on the microsatellite analysis of modern and museum samples
Authors: Abdelmanova Alexandra S , Kharzinova Veronika R , Volkova Valeria , Dotsev Arsen V , Sermyagin Alexander A , Boronetskaya Oxana , Chinarov Roman , Brem Gottfried , Zinovieva Natalia A
Published: October 2021

In their centuries-long history, the nomads of the south of Russia developed the local cattle breeds, which are well adapted to the harsh steppe conditions. In 20th century, the population size of such cattle breeds was dramatically decreased. At the same time, intensive cross breeding with high-producing transboundary breeds had been practiced on the small remaining part of population. The aim of our study was to trace the historical genetic components in modern populations of local cattle breeds of the turano-mongolian origin. The historical specimens of Kalmyk (n = 10), Kyrgyz (n = 11) and Kazakh (n = 3) cattle dated by the first quarter of the 20th century were derived from the craniological collection of the E.F. Liskun Museum for Animal Husbandry and subjected to the study. The modern representatives of Kalmyk (n = 28), Kyrgyz (n = 20), Kazakh White-headed (n = 30), Hereford (n = 26), and Mongolian (n = 41) cattle were used for comparison. All works with historical samples were performed in dedicated facility of the L.K. Ernst research Center for Animal Husbandry. The samples were genotyped for 11 microsatellite loci (BM1818, BM2113, BM1824, ETH10, ETH225, INRA023, SPS115, TGLA53, TGLA122, TGLA126, and TGLA227). For historical samples, the PCR reactions were carried out in five replicates to determine the consensus genotypes. In total, 132 alleles were identified, including 97 alleles in historical samples and 124 alleles in modern samples. The values of unbiased expected heterozygosity were 0.767–0.776 and 0.653–0.778 for the historical and modern samples, respectively. The STRUCTURE clustering showed the visible differences in genetic structure between the historical and modern populations of Kalmyk, Kyrgyz and Kazakh cattle breeds; however, the historical genetic components were still maintained in modern representatives of all of studied breeds. The research results will be useful for the sustainable breeding and conservation of valuable local genetic resources. The study was funded by the RSF No. 19-76-20012.
PSXV-6 The effect of ultrafine iron particles on the adaptation of the digestive system of cattle to fat diets
Authors: Klimova Tatyana , Shejjda Elena , Shajahmetova Elvina , Lebedev Svyatoslav , Miroshnikov Sergej , Rakhmatullin Shamil , Kvan Olga
Published: October 2021

The aim of our study was to determine the possibility of using ultrafine Fe particles (2.2 mg/head/day) as modulators of metabolic activity when using vegetable soybean and sunflower oils in ruminant diets. The studies carried out on calves with external duodenal anastomosis (diet – grassland hay (2 kg), a mixture of concentrates (1.5 kg), corn silage (5 kg), wheat straw (1 kg), oil-3% of the dry matter of the diet). The digestibility of the nutritional components of the diet analyzed daily throughout the study period. The collection of pancreatic juice was carried out for 8 hours with an interval of 60 minutes on the 7th day, the determination of the amount and enzymatic activity of the juice carried out “incito.” Introduction of ultrafine Fe particles significantly increased the digestibility of organic matter by 38% (P ≤ 0.05) with the introduction of vegetable soybean oil, crude fat by 28.4% (P ≤ 0.05) with the introduction of sunflower oil. Additional introduction of ultrafine Fe particles significantly increased the level of pancreatic secretion when sunflower oil was introduced by 59.8%, soybean oil by 56% (P ≤ 0.05). Additional inclusion of ultrafine iron particles contributed to the decrease of lipase, protease and amylase activity by 86,8%, 37,4% and 33% (P ≤ 0,05) in the sunflower oil group, by 68,2% of lipase, by 24,6% of amylase in the soybean oil group (P ≤ 0,05). A significant increase in the level of NO-metabolites was recorded in the group receiving soybean oil by 37.5 % (P < 0.05). This study shows the potential use of ultrafine Fe particles as a mineral supplement to regulate the functional activity of digestive and metabolic processes in ruminants, which may play a positive role in increasing the bioavailability of nutritional components of diets. This research performed with financial support from the project 0761-2019-0005.
PSIX-12 “Lamarin Saldonum” for the raising of milk production of cows
Authors: Anikin Sergey , Shemuranova Natalia , Garifullina Natalia , Filatov Andrey , Sapozhnikov Alexander
Published: October 2021

The aim of the research was to establish the effectiveness of the use of the feed additive “Lamarin Saldonum.” The feed additive contains Japanese kelp, milk thistle, to increase the milk productivity during the milking period. The studies were conducted on 40 cows of the black-and-white Holstein breed at the age of 2–4 lactation and the amount of milk for the last lactation not less than 7,500 kg. The cows were divided into 4 groups (GE1, GE2, GE3, GC) of 10 heads in each. Cows GE1, GE2, GE3 received 30 days before calving daily feed additive in doses of 0.1, 0.2, 0.3 g/kg, respectively, for 60 days. In the GC animals didn’t receive the feed additive (control group). Control milking was performed every 10 days. Using of feed additive in the ration increased milk productivity. In the 1st month of lactation, the average daily amount of milk in GC was 24.00±0.93 kg, which is 7.4%, 19.6% (P < 0.01), 7.4% less than in GE1, GE2 and GE3, respectively. In the 2nd month, the highest average daily amount of milk in GE2 and GE3, which is 13.4% (P < 0.01) and 4.6% more than the GC, respectively. In the 3rd month, the average daily amount of milk in GE1, GE2, and GE3 was 30.22±2.44 kg, 39.57±2.68 kg, 35.11±2.59 kg, respectively, which is 6.8%, 39.9% (P < 0.01), 24.1% more than in GC. The mass fraction of fat in milk in animals GE1, GE2, GE3 was 0.2–0.92%(P < 0.01) higher than in GC. In the second month of lactation, the mass fraction of protein in GE2 was higher by 0.24% (P < 0.01), in GE3–by 0.14 (P < 0.05) relative to GC. At the 3rd month of lactation, the protein content in milk was 0.29% higher in GE2 (P < 0.001) and GE3-by 0.21% (P < 0.001) than in GC.
PSIX-13 Activation of cell signaling pathways by galectins in cow blood
Authors: Rehrah Djaafar M , Worku Mulumebet , Ismail Hamid
Published: October 2021

Galectins are part of a conserved family of β-galactoside-binding proteins that contribute to critical biological events during mammalian gestation and increasingly recognized for a possible role in the immune response of the cow. The objective of this study was to evaluate the effect of Galectins on signal transduction and cell activation in cow blood. Whole blood was collected aseptically from the jugular vein of healthy Holstein Friesian dairy cows (N=3). Blood samples (2.5ml) in duplicate were treated with 150µl of the four different type of recombinant galectins (1, 3, 4, and 9) respectively and untreated samples were served as control. The concentration of total plasma protein was determined using the Pierce BCA kit. Protein expression profiling was performed using1,358 antibodies on the Full Moon BioSystems’ Signaling Explorer antibody array covering 20 cell signaling pathways, as recommended by the manufacturer using an Agilent microarray scanner. Data normalization was performed using GeneSpring GX software to generate fold changes in gene expression and then filtered to obtain a list of significantly upregulated and downregulated genes. Features were extracted from protein array images of samples treated with Galectin 1, 3, 4, 9, and untreated sample as a control group. Treatment with all four Galectins increased the concentration of total plasma protein. Average increases due to treatment with Gal1, Gal3, Gal4, and Gal9 were 27%, 10%, 20%, and 14% respectively. ANOVA test showed significance difference among the groups (p < 0.05). Dunnett option was used to compare each of the treated samples to the control group as a baseline. The results also showed that there was significant difference between the control group and any of the treated group (p < 0.05). Distinct signaling pathways are activated in response to Galectin exposure. Further studies are needed to define their regulation and functional impact on cow health.
PSIX-16 Association between Thrombin and horn fly abundance in beef cattle
Authors: Krause Taylor , Ling Ashley S , Warner Amanda , Duggin Jason , Heins Bradley , Hinkle Nancy , Aggrey Samuel E , Pringle Dean , Rekaya Romdhane
Published: October 2021

It has been reported that differences in horn fly tolerance in beef cattle could be associated with variation in blood enzymes. Thrombin (TH), a major enzyme for hemostasis that plays an important role in the activation of several pro-coagulation factors through the conversion of fibrinogen to fibrin, has been associated with horn fly prevalence on cattle. Although this assumption is reasonable, as fast-coagulating blood tends to complicate the feeding process for flies, there is little meaningful field data that clearly supports this hypothesis. Therefore, the objective of this study was to assess TH levels in relation to horn fly abundance using 360 cows at two University of Georgia farms (Northwest Georgia REC, 34.5°N, 85.0°W and Eatonton Beef Research Unit, 33.4°N, 83.5°W). Horn fly counts were taken twice by trained evaluators during the summer of 2019 (June and August). Additionally, blood samples were collected twice on each cow to quantify serum TH levels using an ELISA assay (MyBioSource, San Diego, CA). The Pearson correlation between serum TH level and horn fly count was -0.19, indicating a decrease in the number of flies with increasing TH in the blood. In order to reduce the impact of the uncertainty associated with the subjective assessment of horn fly abundance and the potential nonlinear relationship between both parameters, TH was categorized into four classes (1=TH >500 ng/mL; 2=250< TH< 500 ng/mL; 3=100< TH< 250 ng/mL; 4=< 100 ng/mL). Average fly counts were 336, 353, 391, and 464 for the four classes, respectively. Between the first (high TH) and fourth (low TH) classes, there was a 38% reduction in fly abundance. Across sire families, fly abundance ranged between 190 and 650. Collectively, these results indicate that selection for higher serum TH levels could be used as a proxy for improving horn fly tolerance in beef cattle.
PSX-B-30 Study of the effects of gestational and weaning stresses on blood cytokines and analytes in pigs
Authors: Rymut Haley E , Rund Laurie A , Bolt Courtni R , Johnson Rodney W , Rodriguez-Zas Sandra L
Published: October 2021

The effects of the practice of weaning in pigs have been documented, and include changes in organ function and immune mechanisms. However, the effects of weaning following exposure to maternal infection during gestation are incompletely characterized. We hypothesize that the disruption of physiological processes triggered by weaning can be influenced by the exposure of the pig to maternal immune signals elicited by a challenge during gestation. To test our hypothesis, the concentrations of biochemical indicators and cytokines were measured in the blood from 72 female and male pigs distributed across two weaning groups (nursed or weaned), and two gestational challenge groups (gilts infected with porcine reproductive and respiratory virus or healthy controls). Weaning had a significant effect on the concentration of albumin among females born from healthy gilts (P < 0.01). The albumin concentrations were higher in weaned relative to nursed pigs from healthy gilts. This pattern may be associated with the transition to a higher protein diet, whereas no differences were observed among pigs from infected gilts. The interaction between gestation and weaning stresses had a significant effect on the concentration of bicarbonate. The highest levels of bicarbonate were detected among nursed pigs from infected gilts. The lowest levels of bicarbonate were detected among weaned pigs from health gilts, and this could indicate metabolic acidosis. An interaction effect (P < 0.02) was detected for pro-inflammatory interleukin 4. The highest concentrations of interleukin 4 were detected in weaned females from infected gilts, while the lowest levels were detected among weaned males from health gilts. The studied biomarker patterns indicate that the effect of weaning on blood indicators can be influenced by the exposure of the pigs to challenges during gestation. This study is supported by USDA NIFA AFRI, grant number 2018-67015-27413.
PSX-B-23 Immune tolerance of dairy heifers in response to repeated bacterial endotoxin exposure
Authors: Sullivan Tianna
Published: October 2021

Lipopolysaccharide (LPS) endotoxin from the membrane of Gram-negative bacteria is often used to simulate bacterial infection in mammals. Repeated exposure to LPS over a short period of time, however, is reported to result in an attenuated acute-phase response. This “LPS tolerance” is an essential immune-homeostatic response that can protect against over-activation of the inflammatory response during chronic exposure to LPS. In the present study, Holstein calves (n=20) were initially intramuscularly challenged with either saline, or 100, 200 or 400 ng/kg of LPS and then all animals were re-challenged with 200 ng/kg of LPS 2-weeks later to assess potential LPS tolerance in ruminants. Serum was collected every 2 hrs for 6 hrs to profile changes in circulatory stress biomarkers. In comparison to the first challenge and saline control animals that received LPS for the first time, heifers re-challenged with LPS demonstrated significantly attenuated cortisol responses in the second LPS challenge (p < 0.05). Blood cell populations were also attenuated in animals receiving their second LPS challenge, in that re-challenged animals showed significantly less severe thrombocytopenic (p < 0.05), and leukopenic responses to their second LPS challenge as compared to their first (p < 0.05); this trend was also apparent when comparing newly LPS-challenged animals to the LPS re-challenged animals. MicroRNA expression profiles were determined to assess a potential epigenetic response to repeated LPS exposure, which may help identify therapeutic targets to protect against LPS-associated diseases including clinical mastitis and sepsis. The present study demonstrated that LPS tolerance occurs in dairy cattle, and understanding the roles of various miRNAs in the context of innate immune cell tolerance is essential for evaluating their impact on immune system homeostasis.
PSX-B-28 Reproductive function of sows with the using of feed additive “Verva”
Authors: Anikin Sergey , Shemuranova Natalia , Filatov Andrey , Sapozhnikov Alexander
Published: October 2021

The aim of the study was to study the effect of the feed additive “Verva” on the reproductive ability of sows and the recovery of their sexual cyclicity. Fir triterpenic acids are the main active ingredient of the feed additive. The study was conducted on 80 sows of the large white breed. Experimental and control groups were formed: GE (n=50), GC (n=30). GE sows from the 80th day of pregnancy for 30 days were additionally given “Verva” at a dose of 3 g per head per day. GC sows weren’t given the “Verva”. Hunting was detected within seven days after weaning the piglets. Artificial insemination was performed twice and the effectiveness was determined on day 28 by ultrasound. The number, weight of newborn piglets in GE and GC don’t differ significantly. By weaning, 10.94±0.17 heads were obtained from one sow in GE, which is 9.1% (p < 0.01) more than in GC. By weaning, the weight of the nest (total weight of piglets) in GC was 66.72=2.13 kg, which is 7.3% (p < 0.05) less than in GE. The “Verva” has a positive effect on the activation of sexual cyclicity: in GE, 95.3% of animals came to the hunt, and in GC-by 4.4% less. On the 4th day after weaning the piglets, 34.1% of the animals came to the hunt in GE, 40% in GC, on the 5th day–46.3% in GE and 50% in GC. On the 6th, 7th days in GE, 17.1% and 2.1% of sows came to the hunt, and in GC, 10.0% and 0.0%, respectively. The efficiency of artificial insemination in GE was 95.4%, which is 13.5% higher than in GC. The “Verva” has a positive effect on the milk production of sows, preserves their progeny, sexual cyclicity after weaning piglets, increases the duration of economic use of animals.