bluuebunny/crossref_metadata_2025 huggingface dataset:
Nanoparticles alleviate non-alcoholic steatohepatitis via ER stress sensor-mediated intestinal barrier damage and gut dysbiosis
Authors: Zhu Manman , Cheng Yong , Tang Yue , Li Shuojiao , Rao Peng , Zhang Guiyang , Xiao Lei , Liu Jiatao
Published: March 2024

Introduction

The gut microbiota plays an important role in the development of non-alcoholic steatohepatitis (NASH), but the underlying mechanism is unclear. It has been found that the transcription factor XBP1s plays an important role in regulating inflammation and lipid metabolism and maintaining the integrity of intestinal barrier. However, whether XBP1s modulates the development of NASH by regulating the integrity of the intestinal barrier and altering the composition of the gut microbiota remains unknown.

Methods

Mice fed with a fat-, fructose-, cholesterol-rich (FFC) diet for 24 weeks successfully established the NASH model, as demonstrated by significant hepatic steatosis, inflammation, hepatocyte injury and fibrosis. The profile of gut microbiota dynamically changed with the different stages of NAFLD via 16S rDNA sequencing the feces from mice fed with FFC diet for 0, 12, or 24 weeks or NASH mice treated with siRNA-loaded folic acid-modified TPGS (hereafter named FT@XBP1).

Results

NASH mice had significantly higher abundance of Firmicutes, Blautia and Bacteroides, and lower abundance of Bifidobacterium and GCA-900066575. FT@XBP1 supplementation had a significantly attenuated effect on FFC diet-induced weight gain, hepatic fat accumulation, dyslipidemia, inflammatory cytokines, ER stress and fibrosis. In particularly, FT@XBP1 modulates the composition of the intestinal flora; for example, NASH mice demonstrated higher abundance of Blautia and Bacteroides, and lower abundance of Actinobacteriota, Muribaculaceae and Bifidobacterium, which were partially restored by FT@XBP1 treatment. Mechanistically, FT@XBP1 increased the expression of ZO-1 in the intestine and had the potential to restore intestinal barrier integrity and improve antimicrobial defense to alleviate enterogenic endotoxemia and activation of inflammatory signaling pathways.

Discussion

Regulation of the key transcription factor XBP1s can partially restore the intestinal microbiota structure, maintain the integrity of intestinal mucosal barrier, and prevent the progression of NASH, providing new evidence for treating NASH.
The contribution of BvgR, RisA, and RisS to global gene regulation, intracellular cyclic-di-GMP levels, motility, and biofilm formation in Bordetella bronchiseptica
Authors: Nicholson Tracy L. , Waack Ursula , Fleming Damarius S. , Chen Qing , Miller Laura C. , Merkel Tod J. , Stibitz Scott
Published: March 2024

Bordetella bronchiseptica is a highly contagious respiratory bacterial veterinary pathogen. In this study the contribution of the transcriptional regulators BvgR, RisA, RisS, and the phosphorylation of RisA to global gene regulation, intracellular cyclic-di-GMP levels, motility, and biofilm formation were evaluated. Next Generation Sequencing (RNASeq) was used to differentiate the global gene regulation of both virulence-activated and virulence-repressed genes by each of these factors. The BvgAS system, along with BvgR, RisA, and the phosphorylation of RisA served in cyclic-di-GMP degradation. BvgR and unphosphorylated RisA were found to temporally regulate motility. Additionally, BvgR, RisA, and RisS were found to be required for biofilm formation.
MicroRNAs participate in the regulation of apoptosis and oxidative stress-related gene expression in rabbits infected with Lagovirus europaeus GI.1 and GI.2 genotypes
Authors: Ostrycharz Ewa , Fitzner Andrzej , Kęsy Andrzej , Siennicka Aldona , Hukowska-Szematowicz Beata
Published: March 2024

MicroRNAs (miRs) are a group of small, 17–25 nucleotide, non-coding RNA that regulate gene expression at the post-transcriptional level. To date, little is known about the molecular signatures of regulatory interactions between miRs and apoptosis and oxidative stress in viral diseases. Lagovirus europaeus is a virus that causes severe disease in rabbits (Oryctolagus cuniculus) called Rabbit Hemorrhagic Disease (RHD) and belongs to the Caliciviridae family, Lagovirus genus. Within Lagovirus europaeus associated with RHD, two genotypes (GI.1 and GI.2) have been distinguished, and the GI.1 genotype includes four variants (GI.1a, GI.1b, GI.1c, and GI.1d). The study aimed to assess the expression of miRs and their target genes involved in apoptosis and oxidative stress, as well as their potential impact on the pathways during Lagovirus europaeus—two genotypes (GI.1 and GI.2) infection of different virulences in four tissues (liver, lung, kidneys, and spleen). The expression of miRs and target genes related to apoptosis and oxidative stress was determined using quantitative real-time PCR (qPCR). In this study, we evaluated the expression of miR-21 (PTEN, PDCD4), miR-16b (Bcl-2, CXCL10), miR-34a (p53, SIRT1), and miRs—related to oxidative stress—miR-122 (Bach1) and miR-132 (Nfr-2). We also examined the biomarkers of both processes (Bax, Bax/Bcl-2 ratio, Caspase-3, PARP) and HO-I as biomarkers of oxidative stress. Our report is the first to present the regulatory effects of miRs on apoptosis and oxidative stress genes in rabbit infection with Lagovirus europaeus—two genotypes (GI.1 and GI.2) in four tissues (liver, lungs, kidneys, and spleen). The regulatory effect of miRs indicates that, on the one hand, miRs can intensify apoptosis (miR-16b, miR-34a) in the examined organs in response to a viral stimulus and, on the other hand, inhibit (miR-21), which in both cases may be a determinant of the pathogenesis of RHD and tissue damage. Biomarkers of the Bax and Bax/Bcl-2 ratio promote more intense apoptosis after infection with the Lagovirus europaeus GI.2 genotype. Our findings demonstrate that miR-122 and miR-132 regulate oxidative stress in the pathogenesis of RHD, which is associated with tissue damage. The HO-1 biomarker in the course of rabbit hemorrhagic disease indicates oxidative tissue damage. Our findings show that miR-21, miR-16b, and miR-34a regulate three apoptosis pathways. Meanwhile, miR-122 and miR-132 are involved in two oxidative stress pathways.
Emerging roles of long non-coding RNAs in osteosarcoma
Authors: Liao Xun , Wei Rong , Zhou Junxiu , Wu Ke , Li Jiao
Published: March 2024

Osteosarcoma (OS) is a highly aggressive and lethal malignant bone tumor that primarily afflicts children, adolescents, and young adults. However, the molecular mechanisms underlying OS pathogenesis remain obscure. Mounting evidence implicates dysregulated long non-coding RNAs (lncRNAs) in tumorigenesis and progression. These lncRNAs play a pivotal role in modulating gene expression at diverse epigenetic, transcriptional, and post-transcriptional levels. Uncovering the roles of aberrant lncRNAs would provide new insights into OS pathogenesis and novel tools for its early diagnosis and treatment. In this review, we summarize the significance of lncRNAs in controlling signaling pathways implicated in OS development, including the Wnt/β-catenin, PI3K/AKT/mTOR, NF-κB, Notch, Hippo, and HIF-1α. Moreover, we discuss the multifaceted contributions of lncRNAs to drug resistance in OS, as well as their potential to serve as biomarkers and therapeutic targets. This review aims to encourage further research into lncRNA field and the development of more effective therapeutic strategies for patients with OS.
Revealing innovative JAK1 and JAK3 inhibitors: a comprehensive study utilizing QSAR, 3D-Pharmacophore screening, molecular docking, molecular dynamics, and MM/GBSA analyses
Authors: Faris Abdelmoujoud , Cacciatore Ivana , Alnajjar Radwan , Hanine Hadni , Aouidate Adnane , Mothana Ramzi A. , Alanzi Abdullah R. , Elhallaoui Menana
Published: March 2024

The heterocycle compounds, with their diverse functionalities, are particularly effective in inhibiting Janus kinases (JAKs). Therefore, it is crucial to identify the correlation between their complex structures and biological activities for the development of new drugs for the treatment of rheumatoid arthritis (RA) and cancer. In this study, a diverse set of 28 heterocyclic compounds selective for JAK1 and JAK3 was employed to construct quantitative structure-activity relationship (QSAR) models using multiple linear regression (MLR). Artificial neural network (ANN) models were employed in the development of QSAR models. The robustness and stability of the models were assessed through internal and external methodologies, including the domain of applicability (DoA). The molecular descriptors incorporated into the model exhibited a satisfactory correlation with the receptor-ligand complex structures of JAKs observed in X-ray crystallography, making the model interpretable and predictive. Furthermore, pharmacophore models ADRRR and ADHRR were designed for each JAK1 and JAK3, proving effective in discriminating between active compounds and decoys. Both models demonstrated good performance in identifying new compounds, with an ROC of 0.83 for the ADRRR model and an ROC of 0.75 for the ADHRR model. Using a pharmacophore model, the most promising compounds were selected based on their strong affinity compared to the most active compounds in the studied series each JAK1 and JAK3. Notably, the pharmacokinetic, physicochemical properties, and biological activities of the selected compounds (As compounds ZINC79189223 and ZINC66252348) were found to be consistent with their therapeutic effects in RA, owing to their non-toxic, cholinergic nature, absence of P-glycoprotein, high gastrointestinal absorption, and ability to penetrate the blood-brain barrier. Furthermore, ADMET properties were assessed, and molecular dynamics and MM/GBSA analysis revealed stability in these molecules.
Research progress on ferroptosis in the pathogenesis and treatment of neurodegenerative diseases
Authors: Wang Lijuan , Fang Xiansong , Ling Baodian , Wang Fangsheng , Xia Yu , Zhang Wenjuan , Zhong Tianyu , Wang Xiaoling
Published: March 2024

Globally, millions of individuals are impacted by neurodegenerative disorders including Huntington's disease (HD), amyotrophic lateral sclerosis (ALS), Parkinson's disease (PD), and Alzheimer's disease (AD). Although a great deal of energy and financial resources have been invested in disease-related research, breakthroughs in therapeutic approaches remain elusive. The breakdown of cells usually happens together with the onset of neurodegenerative diseases. However, the mechanism that triggers neuronal loss is unknown. Lipid peroxidation, which is iron-dependent, causes a specific type of cell death called ferroptosis, and there is evidence its involvement in the pathogenic cascade of neurodegenerative diseases. However, the specific mechanisms are still not well known. The present article highlights the basic processes that underlie ferroptosis and the corresponding signaling networks. Furthermore, it provides an overview and discussion of current research on the role of ferroptosis across a variety of neurodegenerative conditions.
Biophysical modulation and robustness of itinerant complexity in neuronal networks
Authors: Venkadesh Siva , Shaikh Asmir , Shakeri Heman , Barreto Ernest , Van Horn John Darrell
Published: March 2024

Transient synchronization of bursting activity in neuronal networks, which occurs in patterns of metastable itinerant phase relationships between neurons, is a notable feature of network dynamics observed in vivo. However, the mechanisms that contribute to this dynamical complexity in neuronal circuits are not well understood. Local circuits in cortical regions consist of populations of neurons with diverse intrinsic oscillatory features. In this study, we numerically show that the phenomenon of transient synchronization, also referred to as metastability, can emerge in an inhibitory neuronal population when the neurons’ intrinsic fast-spiking dynamics are appropriately modulated by slower inputs from an excitatory neuronal population. Using a compact model of a mesoscopic-scale network consisting of excitatory pyramidal and inhibitory fast-spiking neurons, our work demonstrates a relationship between the frequency of pyramidal population oscillations and the features of emergent metastability in the inhibitory population. In addition, we introduce a method to characterize collective transitions in metastable networks. Finally, we discuss potential applications of this study in mechanistically understanding cortical network dynamics.
Analyzing the bibliometrics of brain-gut axis and Parkinson’s disease
Authors: Chen Lingshan , Chen Jianfei , Wu Min , Yan Pingkang , Zhou Xueping
Published: March 2024

Background

Parkinson’s disease (PD), characterized by the loss of dopaminergic neurons, is a progressive neurodegenerative disorder. Recent research has revealed a significant connection between gut microbiota and PD. To gain insight into research interests, disciplinary contexts, and potential future directions, a comprehensive bibliometric analysis was conducted on the brain-gut axis and PD literature published between 2014 and 2023.

Methods

Relevant literature records were gathered from the Web of Science Core Collection on August 11, 2023. The data were then analyzed by Biblioshiny R packages and VOSviewer (version 1.6.19).

Results

The dataset revealed an upward trend in annual scientific publications on the brain-gut axis and PD, with an annual growth rate of 50.24%. China, the United States, and Italy were the top three most productive countries/regions. The journal “International Journal Of Molecular Sciences” published the most articles, while “Movement Disorders” received the highest number of citations. Professor Keshavarzian A emerged as the most prolific author, while Professor Scheperjans F held the highest h-index. Keyword analysis highlighted “alpha-synuclein” as the most frequent term, with “mouse model,” “inflammation,” and “risk” as emerging research topics. Additionally, “central nervous system” and “intestinal bacterial overgrowth” attracted increasing attention.

Conclusion

This study examined current trends and hotspots in the bibliometric landscape of the brain-gut axis and PD research. Future research directions should explore the functional and metabolic activities of gut microbiota. Additionally, transitioning from observational to interventional study designs offers the potential for personalized interventions and disease prediction. These findings can guide researchers in navigating the latest developments and shaping the future directions of this field.
The efficacy and safety of novel antiepileptic drugs in treatment of epilepsy of patients with brain tumors
Authors: Zhai Weiwei , Yu Qiaoling , Wu Huizhen
Published: March 2024

Objective

This meta-analysis aimed to assess the effectiveness and safety of novel antiepileptic drugs (AEDs) in treating epilepsy in patients with brain tumors (BTRE).

Methods

A search was conducted on PubMed, EMBASE, Web of Science, and the Cochrane Library from inception to February 2023, with English language restriction.

Results

In this meta-analysis, 18 clinical trials involving 755 BTRE patients were included to assess the efficacy and safety of novel AEDs in BTRE treatment. At the last follow-up, a ≥50% reduction in seizure frequency was experienced by 72% of patients (random-effects model, 95% CI = 0.64–0.78) using novel AEDs. At the last follow-up, seizure freedom was experienced by 34% of patients (random-effects model, 95% CI = 0.28–0.41) using novel AEDs. The pooled incidence of AEs was found to be 19% (95% CI: 13%–26%), with a withdrawal rate due to adverse effects of only 3%. Comparable efficacy and incidence of adverse effects were observed between lacosamide and perampanel.

Conclusion

This meta-analysis suggests that novel antiepileptic drugs are deemed effective for seizure control in brain tumor patients, particularly when used as adjunctive therapy. Although lacosamide and perampanel received more focus in studies, no significant difference was observed in the efficacy and adverse reactions of these two drugs in seizure control. Further randomized controlled trials are deemed necessary to validate our findings.
Nomogram prediction model for the risk of intracranial hemorrhagic transformation after intravenous thrombolysis in patients with acute ischemic stroke
Authors: Ma Yong , Xu Dong-Yan , Liu Qian , Chen He-Cheng , Chai Er-Qing
Published: March 2024

Background

Hemorrhagic transformation (HT) after intravenous thrombolysis (IVT) might worsen the clinical outcomes, and a reliable predictive system is needed to identify the risk of hemorrhagic transformation after IVT.

Methods

Retrospective collection of patients with acute cerebral infarction treated with intravenous thrombolysis in our hospital from 2018 to 2022. 197 patients were included in the research study. Multivariate logistic regression analysis was used to screen the factors in the predictive nomogram. The performance of nomogram was assessed on the area under the receiver operating characteristic curve (AUC-ROC), calibration plots and decision curve analysis (DCA).

Results

A total of 197 patients were recruited, of whom 24 (12.1%) developed HT. In multivariate logistic regression model National Institute of Health Stroke Scale (NIHSS) (OR, 1.362; 95% CI, 1.161–1.652; p = 0.001), N-terminal pro-brain natriuretic peptide (NT-pro BNP) (OR, 1.012; 95% CI, 1.004–1.020; p = 0.003), neutrophil to lymphocyte ratio (NLR) (OR, 3.430; 95% CI, 2.082–6.262; p < 0.001), systolic blood pressure (SBP) (OR, 1.039; 95% CI, 1.009–1.075; p = 0.016) were the independent predictors of HT which were used to generate nomogram. The nomogram showed good discrimination due to AUC-ROC values. Calibration plot showed good calibration. DCA showed that nomogram is clinically useful.

Conclusion

Nomogram consisting of NIHSS, NT-pro BNP, NLR, SBP scores predict the risk of HT in AIS patients treated with IVT.
Functional contribution of the intestinal microbiome in autism spectrum disorder, attention deficit hyperactivity disorder, and Rett syndrome: a systematic review of pediatric and adult studies
Authors: Caputi Valentina , Hill Lee , Figueiredo Melanie , Popov Jelena , Hartung Emily , Margolis Kara Gross , Baskaran Kanish , Joharapurkar Papiha , Moshkovich Michal , Pai Nikhil
Published: March 2024

Introduction

Critical phases of neurodevelopment and gut microbiota diversification occur in early life and both processes are impacted by genetic and environmental factors. Recent studies have shown the presence of gut microbiota alterations in neurodevelopmental disorders. Here we performed a systematic review of alterations of the intestinal microbiota composition and function in pediatric and adult patients affected by autism spectrum disorder (ASD), attention-deficit/hyperactivity disorder (ADHD), and Rett syndrome (RETT).

Methods

We searched selected keywords in the online databases of PubMed, Cochrane, and OVID (January 1980 to December 2021) with secondary review of references of eligible articles. Two reviewers independently performed critical appraisals on the included articles using the Critical Appraisal Skills Program for each study design.

Results

Our systematic review identified 18, 7, and 3 original articles describing intestinal microbiota profiles in ASD, ADHD, and RETT, respectively. Decreased Firmicutes and increased Bacteroidetes were observed in the gut microbiota of individuals affected by ASD and ADHD. Proinflammatory cytokines, short-chain fatty acids and neurotransmitter levels were altered in ASD and RETT. Constipation and visceral pain were related to changes in the gut microbiota in patients affected by ASD and RETT. Hyperactivity and impulsivity were negatively correlated with Faecalibacterium (phylum Firmicutes) and positively correlated with Bacteroides sp. (phylum Bacteroidetes) in ADHD subjects. Five studies explored microbiota-or diet-targeted interventions in ASD and ADHD. Probiotic treatments with Lactobacillus sp. and fecal microbiota transplantation from healthy donors reduced constipation and ameliorated ASD symptoms in affected children. Perinatal administration of Lactobacillus sp. prevented the onset of Asperger and ADHD symptoms in adolescence. Micronutrient supplementation improved disease symptomatology in ADHD without causing significant changes in microbiota communities’ composition.

Discussion

Several discrepancies were found among the included studies, primarily due to sample size, variations in dietary practices, and a high prevalence of functional gastrointestinal symptoms. Further studies employing longitudinal study designs, larger sample sizes and multi-omics technologies are warranted to identify the functional contribution of the intestinal microbiota in developmental trajectories of the human brain and neurobehavior.

Systematic review registration

https://clinicaltrials.gov/, CRD42020158734.
Treatment response of advanced HNSCC towards immune checkpoint inhibition is associated with an activated effector memory T cell phenotype
Authors: Schumacher Max , Beer Sina , Moraes Ribeiro Emmanuelle , Korkmaz Fulya , Keppeler Hildegard , Fitzel Rahel , Erkner Estelle , Radszuweit Pia , Lengerke Claudia , Schneidawind Corina , Hoefert Sebastian , Mauz Paul Stefan , Schneidawind Dominik
Published: March 2024

Locally advanced or metastatic head and neck squamous cell carcinoma (HNSCC) is associated with a poor prognosis. The introduction of PD-1 inhibitors has led to a significant improvement in survival, but only a subpopulation of patients responds to therapy. Current biomarkers cannot reliably identify these patients. The identification of biomarkers for the prediction and monitoring of immunotherapy is therefore of great importance. In this study, we characterized lymphocyte subsets in the peripheral blood of HNSCC patients under PD-1 inhibition. Patients with primary response (n=11) to PD-1 inhibition showed an increase of the CD3+ effector memory (CD3/EM) population and an elevated expression of the activation marker CD69 in CD3+ T cells, particularly in the CD3/EM subpopulation at 3 months when treatment response was assessed. In contrast, patients with primary treatment failure and progressive disease (n=9) despite PD-1 inhibition had lower absolute lymphocyte counts and an increased expression of CTLA-4 in CD3+ T cells at the time of treatment failure compared with baseline, particularly in CD4+ and CD8+ effector memory populations. Our results demonstrate that HNSCC patients’ response to immune checkpoint inhibition shows a distinct immune signature in peripheral blood, which could help identify refractory patients earlier. Furthermore, strategies to overcome primary therapy failure by inducing a beneficial T cell phenotype or adding alternative immune checkpoint inhibitors could improve response rates and survival of HNSCC patients.
Global trends in the research on Legg–Calve–Perthes disease in Web of Science
Authors: Qin Wenbao , He Mingwei , Qin Hongsheng , Wei Qingjun , Yan Haiwei
Published: March 2024

Background

Legg–Calve–Perthes disease (LCPD) is a form of idiopathic femoral head necrosis that can lead to permanent femoral head deformities and premature osteoarthritis in children under the age of 15. Its pathogenesis is utterly and remains to be clarified. Although many research publications on LCPD have emerged during the last few decades, few systematic bibliometric analyses of these articles have been reported.

Methods

A bibliometric analysis was performed to investigate the development processes and hotspots, as well as the collaboration and influence among countries, institutions, authors, journals, and keywords of papers relevant to LCPD from the Web of Science Core Collection (WoSCC) during the period from 1 January 2000 to 30 June 2023.

Results

A total of 2,205 researchers from 916 institutions across 53 countries/regions have contributed to 673 papers published in 199 academic journals. The research on LCPD has shown significant fluctuations but a gradual increase in the number of articles published over the last two decades. The United States leads in the number of publications of LCPD, with the Texas Scottish Rite Hospital for Children being the most productive institution. English, as the most widely used language in the world, was undoubtedly the most popular language. Herring JA, who acted as both the corresponding and first author, has contributed to the most co-cited papers published. The most number of LCPD papers are published in the Journal of Pediatric Orthopaedics, whereas the Journal of Bone and Joint Surgery American Volume garnered the highest total citations, indicating the great importance of these two journals in the field of orthopedics. The most frequently used keywords in published articles were related to the symptoms, mechanisms, and prognosis, revealing the research focus of most scholars.

Conclusion

Our research described the development trends and hotspots in the research field of LCPD and will help researchers make better decisions.
Total phenolic contents, cytotoxic, free radicals, porcine pancreatic α-amylase, and lipase suppressant activities of Artemisia dracunculus plant from Palestine
Authors: Jaradat Nidal , Dwikat Majdi , Amer Johnny , Ghanim Mustafa , Hawash Mohammed , Hussein Fatima , Issa Linda , Ishtawe Salsabeel , Salah Shahd , Nasser Sara
Published: March 2024

Artemisia dracunculus: L. (A. dracunculus) is a popular vegetable and spice cultivated across many Middle Eastern countries. The herb’s aqueous extract has significant folkloric medicinal importance for treating various disorders. Hence, the present investigation aimed to investigate A. dracunculus hydrophilic extract phytochemical constituents and pleiotropic biological potentials, as no previous studies have investigated the antilipase and anti-α-amylase effects of the A. dracunculus plant. Total phenol content and phytochemical screening assays were performed utilizing standard analytical methods. While the α-amylase inhibition, free radical-scavenging, antilipase, and cytotoxic activities were determined using dinitrosalicylic acid (DNSA), DPPH, p-nitrophenyl butyrate (PNPB), and MTS assays, respectively. The standard phytochemical analysis of A. dracunculus aqueous extract shows that this extract contains only a phenolic group. The total phenol content was 0.146 ± 0.012 mg GAE/g of the plant dry extract. The A. dracunculus aqueous extract exhibited potent DPPH free radical inhibitory (IC50 dose of 10.71 ± 0.01 μg/mL) and anti-lipase activities (IC50 dose of 60.25 ± 0.33 μg/mL) compared with Trolox (IC50 = 5.7 ± 0.92 μg/mL) and Orlistat (IC50 = 12.3 ± 0.35 μg/mL), respectively. However, it showed a weak anti-α-amylase effect (IC50 value > 1,000 μg/mL) compared with Acarbose (IC50 = 28.18 ± 1.27 μg/mL). A. dracunculus has a cytotoxic effect against the HeLa cancer cell line compared with the chemotherapeutic agent Doxorubicin. The extract has the same percent of inhibition as Doxorubicin (99.9%) at 10 mg/mL. Overall, these results pointed out for the first time the importance of considering A. dracunculus effects as a favorite candidate for preventing and treating metabolic disorders. Also, our results confirm the findings of previous reports on the role of A. dracunculus in the management of cancer and disorders resulting from the accumulation of harmful free radicals. On the contrary, the current study concluded that the antidiabetic role of A. dracunculus could be minimal. Further in-depth investigations are urgently warranted to explore the importance of A. dracunculus in pharmaceutical production.
The role of inflammation in silicosis
Authors: Liu Tong-Tong , Sun Hai-Fei , Han Yan-Xing , Zhan Yun , Jiang Jian-Dong
Published: March 2024

Silicosis is a chronic illness marked by diffuse fibrosis in lung tissue resulting from continuous exposure to SiO2-rich dust in the workplace. The onset and progression of silicosis is a complicated and poorly understood pathological process involving numerous cells and molecules. However, silicosis poses a severe threat to public health in developing countries, where it is the most prevalent occupational disease. There is convincing evidence supporting that innate and adaptive immune cells, as well as their cytokines, play a significant role in the development of silicosis. In this review, we describe the roles of immune cells and cytokines in silicosis, and summarize current knowledge on several important inflammatory signaling pathways associated with the disease, aiming to provide novel targets and strategies for the treatment of silicosis-related inflammation.
Auto-verbalizer filtering for prompt-based aspect category detection
Authors: Xian Yantuan , Qin Yuan , Xiang Yan
Published: March 2024

Aspect category detection (ACD) is a basic task in sentiment analysis that aims to identify the specific aspect categories discussed in reviews. In the case of limited label resources, prompt-based models have shown promise in few-shot ACD. However, their current limitations lie in the manual selection or reliance on external knowledge for obtaining the verbalizer, a critical component of prompt learning that maps predicted words to final categories. To solve these issues, we propose an ACD method to automatically build the verbalizer in prompt learning. Our approach leverages the semantic expansion of category labels as prompts to automatically acquire initial verbalizer tokens. Additionally, we introduce an indicator mechanism for auto-verbalizer filtering to obtain reasonable verbalizer words and improve the predicting aspect category reliability of the method. In zero-shot task, our model exhibits an average performance improvement of 7.5% over the second-best model across four ACD datasets. For the other three few-shot tasks, the average performance improvement over the second-best model is approximately 2%. Notably, our method demonstrates effectiveness, particularly in handling general or miscellaneous category aspects.
BmC/EBPZ gene is essential for the larval growth and development of silkworm, Bombyx mori
Authors: Mei Xinglin , Huang Tianchen , Chen Anli , Liu Weibin , Jiang Li , Zhong Shanshan , Shen Dongxu , Qiao Peitong , Zhao Qiaoling
Published: March 2024

The genetic male sterile line (GMS) of the silkworm Bombyx mori is a recessive mutant that is naturally mutated from the wild-type 898WB strain. One of the major characteristics of the GMS mutant is its small larvae. Through positional cloning, candidate genes for the GMS mutant were located in a region approximately 800.5 kb long on the 24th linkage group of the silkworm. One of the genes was Bombyx mori CCAAT/enhancer-binding protein zeta (BmC/EBPZ), which is a member of the basic region-leucine zipper transcription factor family. Compared with the wild-type 898WB strain, the GMS mutant features a 9 bp insertion in the 3′end of open reading frame sequence of BmC/EBPZ gene. Moreover, the high expression level of the BmC/EBPZ gene in the testis suggests that the gene is involved in the regulation of reproduction-related genes. Using the CRISPR/Cas9-mediated knockout system, we found that the BmC/EBPZ knockout strains had the same phenotypes as the GMS mutant, that is, the larvae were small. However, the larvae of BmC/EBPZ knockout strains died during the development of the third instar. Therefore, the BmC/EBPZ gene was identified as the major gene responsible for GMS mutation.
Associations between olfactory reference disorder and social phobia – results of an internet-based study
Authors: Reuter Julia , Grocholewski Anja , Steil Regina
Published: March 2024

Despite the similar clinical features of Olfactory Reference Disorder (ORD) and Social Phobia (SP), or studies showing elevated comorbidity of the two disorders, and the conceptualization of ORD as a form of SP in the East Asian culture, to our knowledge, the relationship between ORD and SP has not been investigated. This study examined the association of ORD according to the 11th revision of the International Classification of Diseases (ICD-11) and SP in 225 German university / college students who completed self-ratings with regard to socio-demographic data and symptoms of SP and ORD within an anonymous internet-based survey. Symptoms of SP were assessed with the Social Phobia Inventory (SPIN). Symptoms of ORD according to the ICD-11 were assessed with the Olfactory Reference Disorder Questionnaire (ORDQ), developed for this study. In our sample, 86.6% of the participants who met the self-rated features for ORD also met the self-rated criteria for current SP. ORD severity scores were significantly related to SP. Participants with and without self-reported ORD differed significantly in their SP total scores. SP severity was also significantly correlated with poorer insight of ORD-related beliefs, greater ORD-related avoidance of intimate relationships and higher levels of shame and fear of rejection due to body odor. These preliminary findings indicate that ORD could be closely related to SP and highlight the need for future research on the relationship of ORD and SP in order to gain a better understanding of the development, maintenance, treatment and classification of ORD.
Efficacy of eHealth interventions to reduce depression symptoms in individuals with obesity: a systematic review of randomized controlled trials
Authors: Kocol Dilara , Bäuerle Alexander , Schadendorf Theresa , Geiger Sheila , Krakowczyk Julia Barbara , Skoda Eva-Maria , Teufel Martin
Published: March 2024

Introduction

Obesity and depression are inter-related health concerns, demanding a high level of treatment and costs in the health care system. The development of eHealth interventions that simultaneously address obesity and mental health can be supportive in this regard. However, evidence of the efficacy of eHealth interventions in the treatment of depression symptoms in individuals with obesity is lacking. The aim of this systematic literature review is to evaluate the efficacy of existing eHealth interventions for individuals with obesity that target depression symptoms.

Methods

We systematically searched electronic databases (Cochrane Library, PubMed, Scopus) to identify studies published in English between January 2016 and January 2023, that focused on eHealth interventions, targeting depression symptoms in individuals with obesity people. Exclusion criteria were study objectives that (1) focused specifically on one or more metabolic comorbidities of individuals with obesity, e.g., hypertension, hyperlipidemia, diabetes; (2) focused specifically on eating disorders comorbidities e.g., binge eating disorder, and (3) focused specifically on patients before or after bariatric surgery.

Results

The database search identified 214 records. Six articles were included in this review. Sample sizes ranged from 70 to 1267 participants of ages 18-60 years. All included studies were randomized controlled trials. Two of the six included studies were web-based interventions guided either by medical doctors or psychologists. All interventions included video, printed materials, and interactive parts of which two studies integrated elements of Cognitive Behavioural Therapy and Social Cognitive Therapy. The findings showed that eHealth treatment services, supported and guided throughout the intervention had high acceptance and efficacy in the reduction of depression symptoms among individuals with obesity.

Conclusion

EHealth interventions that address and target both mental and physical health with interactive strategies calls for better efficacy in the reduction of depression symptoms. Future eHealth interventions that target depression symptoms in individuals with obesity should integrate digital strategies that address both mental and physical health through interactive modules.
Work–family conflict categories and support strategies for married female nurses: a latent profile analysis
Authors: Yao Xin , Wen Siqi , Song Ziling , Wang Jing , Shen Yuanyuan , Huang Xiaoqiong
Published: March 2024

Objective

To clarify subgroups of married female nurses experiencing work–family conflict (WFC), explore the factors associated with the subgroups, and determine how desired support strategies differ among the subgroups.

Methods

Data was collected from a sample of 646 married female nurses from public hospitals in Zhejiang Province, China, in December 2021. Latent profile analysis was used to group the participants, and multiple logistic regression was used to identify factors associated with higher WFC. The STROBE criteria were used to report results.

Results

According to latent profile analysis, there were three distinct profiles of WFC among married female nurses: “low-conflict type,” “work-dominant-conflict type,” and “high-conflict type.” These profiles differed in the number of children, night shifts, family economic burden, childcare during working hours, family harmony, colleague support, and nurse–patient relationships. Nurses with multiple children, higher pressures in childcare during working hours, heavier family economic burdens, lower family harmony, lower colleague support, and poorer nurse–patient relationships are more likely to be classified as “high-conflict type” nurses.

Conclusion

This study found that married female nurses experience different types of WFCs. The structure of these WFCs and their associated factors suggests that customized intervention strategies can be developed to address the specific needs of married female nurses.
Digital twins in sustainable transition: exploring the role of EU data governance
Authors: Garske Beatrice , Holz Wilmont , Ekardt Felix
Published: March 2024

Introduction

Digital twins can accelerate sustainable development by leveraging big data and artificial intelligence to simulate state, reactions and potential developments of physical systems. In doing so, they can create a comprehensive basis for data-driven policy decisions. One of the purposes of digital twins is to facilitate the implementation of the EU's Green Deal-in line with internationally binding climate and environmental targets. One prerequisite for the success of digital twins is a comprehensive, high-quality database. This requires a suitable legal framework that ensures access to such data.

Methods

Applying a qualitative governance analysis, the following article examines if the EU's strategies and legal acts on data governance are paving the way for digital twin projects which promote sustainability.

Results

Results show important starting points for open and fair data use within the growing field of EU digital law. However, there is still a lot of progress to be made to legally link the use of digital twins with binding sustainability objectives.
Assessing anaerobic microbial degradation rates of crude light oil with reverse stable isotope labelling and community analysis
Authors: Beilig Sebastian , Pannekens Mark , Voskuhl Lisa , Meckenstock Rainer U.
Published: March 2024

Oil reservoirs represent extreme environments where anaerobic degradation profoundly influences oil composition and quality. Despite the common observation of biodegraded oil, the microbial degradation rates remain largely unknown. To address this knowledge gap, we conducted microcosm incubations with light oil as carbon source, original formation water and sulfate as electron acceptor, closely mimicking in situ conditions to assess oil degradation rates. Samples were taken from a newly drilled oil well to exclude contamination with injection water and allochthonous microorganisms. At the end of the incubations, microbial community analyses with 16S rRNA gene amplicon sequencing revealed the most prominent phyla as Desulfobacterota, Thermotogota, Bacteroidota, Bacillota (formerly Firmicutes), and Synergistota, collectively accounting for up to 44% of relative abundance. Ion chromatography and reverse stable isotope labeling were used to monitor sulfate reduction and CO2 evolution respectively. We calculated an average degradation rate of 0.35 mmol CO2 per year corresponding to 15.2 mmol CO2/mol CH2(oil) per year. This resembles to approximately 200 years to degrade one gram of oil under the applied, presumably ideal conditions. Factoring in the available oil-water-contact (OWC) zone within the incubations yielded a degradation rate of 120 g CH2 m−2 OWC per year, closely aligning with the modeled degradation rates typically observed in oil reservoirs. Moreover, our study highlighted the utility of the reverse stable isotope labeling (RSIL) approach for measuring complex substrate degradation at minute rates.
Nutrition security and traditional food markets in Africa: gender insights
Authors: de Kanter Emilie , Trevenen-Jones Ann , Billiard Charlie M. J.
Published: March 2024

Malnutrition is a major global challenge. Multiple forms, from underweight to obesity, exist, and several forms coexist within communities and households. Traditional food markets, also known as wet, local, or informal markets, are widespread in sub-Saharan Africa (SSA) and are a key place where people buy and sell food and socially interact, especially those vulnerable to malnutrition. As such they are vital to food and nutrition security. While it is recognized that gender is an important consideration in food and nutrition security, very little has been published in peer reviewed journals, with respect to gender and traditional food markets in SSA. This mini review aims to explore the nexus role of traditional food markets and gender in food and nutrition security. This study presents a narrative literature review, informed by literature identified in a systematic manner. Four databases were searched for key terms, including nutrition, different forms of malnutrition, gender, traditional food markets, and vendors. The papers provided insight into two main topics pertaining to the role of traditional food market practices, gender, and food and nutrition security. While few papers were identified in this mini review, they illustrated insightful nuances into traditional food markets, gender, and food and nutrition security. There is a need for explicitly framed gender studies that can better inform the limited existing knowledge of the experiences of gender and nutritional security of women and men in traditional food markets in SSA.
Agricultural socialized services empowering smallholder rice producers to achieve high technical efficiency: empirical evidence from southern China
Authors: Cai Baozhong , Shi Fang , Meseretchanie Abate , Betelhemabraham Geremew , Zeng Rong
Published: March 2024

Introduction

This study emphasizes the importance of agricultural efficiency for food security and income generation, especially among smallholder rice farmers in southern China. Limited access to essential agricultural services hinders productivity in this region. The study highlights the significant potential of agricultural socialized services (ASS) in improving the technical efficiency of smallholder rice production.

Methods

To analyze the impact of ASS on technical efficiency in rice production, we focused on tillage, transplanting, crop protection, and harvest operations. We employed stochastic frontier analysis and collected data from smallholder farmers in Hunan, Jiangxi, and Zhejiang provinces. By estimating the technical efficiency of rice production, we aimed to assess the relationship between ASS and smallholder farmers’ technical efficiency.

Results and discussion

The results of our analysis revealed that ASS significantly enhance the technical efficiency of rice production among smallholder farmers by supporting agricultural practices such as transplanting, crop protection, and harvest operations. However, we found that the impact of ASS on tillage operations was not statistically significant. Participation in ASS enhances smallholders’ access to modern production techniques, resources, and knowledge, leading to improved technical efficiency. These services also empower smallholder rice producers to adopt sustainable farming practices, access credit, financing, and market information, and promote collective action and cooperation, ultimately influencing technical efficiency.

Conclusion

This study emphasizes the potential of ASS in improving the technical efficiency of smallholder rice production in southern China. Policymakers and agricultural organizations can use these insights to design interventions that promote efficient practices, enhance productivity, support livelihoods, and ensure food security in the region.
Exploring Control Authority Preferences in Robotic Arm Assistance for Power Wheelchair Users
Authors: Styler Breelyn Kane , Deng Wei , Simmons Reid , Admoni Henny , Cooper Rory , Ding Dan
Published: March 2024

This paper uses mixed methods to explore the preliminary design of control authority preferences for an Assistive Robotic Manipulator (ARM). To familiarize users with an intelligent robotic arm, we perform two kitchen task iterations: one with user-initiated software autonomy (predefined autonomous actions) and one with manual control. Then, we introduce a third scenario, enabling users to choose between manual control and system delegation throughout the task. Results showed that, while manually switching modes and controlling the arm via joystick had a higher mental workload, participants still preferred full joystick control. Thematic analysis indicates manual control offered greater freedom and sense of accomplishment. Participants reacted positively to the idea of an interactive assistive system. Users did not want to ask the system to only assist, by taking over for certain actions, but also asked for situational feedback (e.g., ‘How close am I (the gripper)?’, ‘Is the lid centered over the jug?’). This speaks to a future assistive system that ensures the user feels like they drive the system for the entirety of the task and provides action collaboration in addition to more granular situational awareness feedback.
Design and Experiment of Oil-Electric Hybrid Air-Suction Sorghum Plot Seeder
Authors: Yuan Xinyu , Huang Caojun , Tao Guixiang , Yi Shujuan , Li Yifei
Published: March 2024

To tackle the issues of low seeding accuracy and seed injury caused by the seeders utilized at a small scale and in the plot seeding of sorghum in mountainous or hilly regions, this study presents the design of an oil–electric hybrid air suction sorghum plot seeder. The main working parts of the seeder are described, and the performance of the seed-mixing device is simulated using EDEM software. An oil–electric hybrid drive mode is used to provide power for operation and to the seed-metering device and fan. Additionally, a sowing control and monitoring system is designed using a single-chip microcomputer controller to ensure uniform plant spacing at different forward speeds. A multi-factor experiment is conducted using the central synthesis method to determine the optimal operating parameters of the seed-metering device through bench tests. The results show that a profile hole diameter of 2.5 mm on the seed tray, a negative-pressure chamber vacuum of 8.0 kPa, and a seed-metering device speed of 28 r/min result in a 95.95% pass rate, 0.5% missing rate, and 3.55% reseeding rate. The deviation between the experimental and analytical results that validate the optimum parameters is kept within acceptable limits. Field tests are conducted at different forward speeds using the optimum parameter combinations, and a comparison is made with the widely used duckbill planter. The results show pass, missing, and reseeding rates of 94.41%, 2.3%, and 3.29%, respectively. The missing monitoring error is less than 7.19%. All of the indices of the oil–electric hybrid air suction sorghum plot seeder are superior to those of the duckbill planter; thus, it fulfills the agronomic requirements for seeding a sorghum plot.