bluuebunny/crossref_metadata_2025 huggingface dataset:
Validation of an LC–HRMS Method for Quantifying Indoxyl Sulfate and p-Cresyl Sulfate in Human Serum
Authors: Rodríguez-García María , Martínez Irene , Aliart Irene , Sainz de Medrano Jaime I. , Rico Nayra , Escudero-Saiz Víctor Joaquín , Maduell Francisco , Morales-Ruiz Manuel , Casals Gregori
Published: February 2025

Accurate quantification of indoxyl sulfate (IndS) and p-cresyl sulfate (pCS) is essential for understanding their role in chronic kidney disease (CKD) progression and for developing strategies to mitigate their harmful effects, including cardiovascular morbidity and renal fibrosis. Advances in liquid chromatography–high-resolution mass spectrometry (LC–HRMS) enable the integration of powerful diagnostic tools into clinical laboratories. Along with accurate quantification, precise mass measurements allow for untargeted compound identification. Methods. An LC–HRMS was validated for quantifying IndS and pCS in human serum, following EMA guidelines. The method involved protein precipitation with methanol, micro-LC for chromatographic separation, and detection based on accurate mass, with simultaneous high-resolution full-scan acquisition. Clinical samples from patients with varying degrees of renal insufficiency and samples obtained before and after hemodiafiltration were analyzed. Results. The method demonstrated acceptable linearity, precision, and accuracy. The measurement range for both analytes was from 100 to 40,000 ng/mL. Serum levels of IndS and pCS correlated with decreased renal function. After hemodiafiltration, there was a significant reduction of IndS (50%) and pCS (43%). Simultaneous untargeted analysis allowed to identify metabolites significantly underexpressed after hemodiafiltration. Conclusions. An accurate LC–HRMS method was validated for the quantification of IndS and pCS serum levels in patients with CKD, providing insights into toxin dynamics and enabling untargeted metabolic evaluation.
Iodine(III)-Containing Reagents in Photo-Assisted and Photo-Catalyzed Organic Synthesis
Authors: Ibarra-Gutiérrez Jaime G. , Segura-Quezada Luis A. , Hernández-Velázquez Edson D. , García-Dueñas Ana K. , Millán-Cortés José A. , Mondragón-Hernández Kevin , Miranda-Navarrete Luz K. , Valtierra-Camarena Evelyn M. , Yebra-Rivera Steffi Y. , Alférez-Carmona Omar E. , Ávalos-Otero Oliver E. , Chávez-Rivera Rubén , de León-Solís Claudia , Ortíz-Alvarado Rafael , Solorio-Alvarado César R.
Published: February 2025

Iodine(III) reagents have become a highly relevant tool in organic synthesis due to their great versatility as strong but green oxidants. Several transformations involving cyclizations as well as functionalization of different organic cores have been broadly described and reviewed. Herein, the participation of these reagents in photochemical transformations exclusively by direct irradition or in photoredox cycles using some transition metals, will be briefly described as well as some plausible further transformations that potentially can be developed.
Towards Completion of the “Periodic Table” of Di-2-Pyridyl Ketoxime
Authors: Stamou Christina , Polyzou Christina D. , Lada Zoi G. , Konidaris Konstantis F. , Perlepes Spyros P.
Published: February 2025

The oxime group is important in organic and inorganic chemistry. In most cases, this group is part of an organic molecule possessing one or more donor sites capable of forming bonds to metal ions. One family of such compounds is the group of 2-pyridyl (aldo)ketoximes. Metal complexes of 2-pyridyl oximes continue to attract the intense interest of many inorganic chemistry groups around the world for a variety of reasons, including their interesting structures, physical and biological properties, and applications. A unique member of 2-pyridyl ketoximes is di-2-pyridyl ketoxime (dpkoxH), which contains two 2-pyridyl groups and an oxime functionality that can be easily deprotonated giving the deprotonated ligand (dpkox−). The extra 2-pyridyl site confers a remarkable flexibility resulting in metal complexes with exciting structural and reactivity features. Our and other research groups have prepared and characterized many metal complexes of dpkoxH and dpkox− over the past 30 years or so. This work is an attempt to build a “periodic table” of dpkoxH, which is near completion. The filled spaces of this “periodic table” contain metal ions whose dpkoxH/dpkox− complexes have been structurally characterized. This work reviews comprehensively the to-date published coordination chemistry of dpkoxH with emphasis on the syntheses, reactivity, relationship to metallacrown chemistry, structures, and properties of the metal complexes; selected unpublished results from our group are also reported. The sixteen coordination modes adopted by dpkoxH and dpkox− have provided access to monomeric and dimeric complexes, trinuclear, tetranuclear, pentanuclear, hexanuclear, heptanuclear, enneanuclear, and decanuclear clusters, as well as to a small number of 1D coordination polymers. With few exceptions ({MIILnIII2} and {NiII2MnIII2}; M = Ni, Cu, Pd, and Ln = lanthanoid), most complexes are homometallic. The metals whose ions have yielded complexes with dpkoxH and dpkox− are Cr, Mn, Fe, Co, Ni, Cu, Zn, Ru, Rh, Pd, Ag, Cd, Re, Os, Ir, Au, Hg, lanthanoids (mainly Pr and Nd), and U. Most metal complexes are homovalent, but some mixed-valence Mn, Fe, and Co compounds have been studied. Metal ion-assisted/promoted transformations of dpkoxH, i.e., reactivity patterns of the coordinated ligand, are also critically discussed. Some perspectives concerning the coordination chemistry of dpkoxH and research work for the future are outlined.
[Tc(NO)Cl2(PPh3)2(CH3CN)] and Its Reactions with 2,2′-Dipyridyl Dichalcogenides
Authors: Sawallisch Till Erik , Rupf Susanne Margot , Abdulkader Abdullah , Ernst Moritz Johannes , Roca Jungfer Maximilian , Abram Ulrich
Published: February 2025

The sparingly soluble technetium(I) complex [TcI(NO)Cl2(PPh3)2(CH3CN)] (1) slowly dissolves during reactions with 2,2′-dipyridyl ditelluride, (2-pyTe)2, 2,2′-dipyridyl diselenide, (2-pySe)2, or 2,2′-dipyridyl disulfide, (2-pyS)2, under formation of deeply colored solutions. Blue (Te compound) or red solids (Se compound) of the composition [{TcI(NO)Cl2(PPh3)2}2{µ2-(2-pyE)2}], E = Te (3), Se (4), precipitate from the reaction solutions upon addition of toluene. They represent the first technetium complexes with dichalcogenides. While [{TcI(NO)Cl2(PPh3)}2{µ2-(2-pyTe)2}] (3) is the sole product, a small amount of a second product, [TcII(NO)Cl2(PPh3)(2-pySe)] (5), was obtained from the respective mother solution of the reaction with the diselenide. From the corresponding reaction between 1 and (2-pyS)2, the technetium(II) compound, [TcII(NO)Cl2(PPh3)(2-pyS)] (6), could be isolated exclusively. The products were studied by single-crystal X-ray diffraction and spectroscopic methods including 99Tc NMR for the technetium(I) products and EPR spectroscopy for the Tc(II) complexes. The experimental results are accompanied by DFT considerations, which help to rationalize the experimental observations.
High Sensitivity and Selectivity of PEDOT/Carbon Sphere Composites for Pb2+ Detection
Authors: Ma Lirong , Wang Zhuangzhuang , Liu Xiong , Xu Feng , Abdiryim Tursun
Published: February 2025

Heavy metal ions impair human health and irreversibly damage the ecosystem. As a result, it is critical to create an efficient approach for identifying heavy metal ions. The electrochemical sensor method is a type of detection method that is highly sensitive, low in cost, and allows for real-time monitoring. In this study, solid carbon spheres were made using resorcinol and formaldehyde as raw materials, followed by the formation of PEDOT/carbon sphere composites via in situ oxidative polymerization, and Pb2+ was detected utilizing them as electrode modification materials. The structure of the PEDOT/carbon spherical composites was analyzed using scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). To investigate the electrochemical properties of these composites, electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and differential pulse voltammetry (DPV) were employed. In addition, the detection mechanism of the material for Pb2⁺ was studied using CV. The PEDOT/carbon sphere sensor showcased an extensive linear detection range of 7.5 × 10−2 to 1.0 μM for Pb2+ ions, achieving a low limit of detection (LOD) of 3.5 × 10−2 nM and displaying exceptional selectivity. These results can be attributed to its large surface area, superior electrical conductivity, and outstanding electron transport properties. This study offers an effective material for detecting low concentrations of Pb2+, with potential applications in future Pb2+ detection.
Bufadienolide Penetration Through the Skin Membrane and Antiaging Properties of Kalanchoe spp. Juices in Dermal Applications
Authors: Hering Anna , Cal Krzysztof , Kowalczyk Mariusz , Kastsevich Alina , Ivashchanka Yahor , Ochocka J. Renata , Stefanowicz-Hajduk Justyna
Published: February 2025

Skin aging is accelerated by inflammation processes generated by oxidative stress and external factors such as UV radiation. Plants belonging to the genus Kalanchoe that are rich sources of antioxidants could potentially strengthen the skin barrier if used as ingredients in cosmetic formulations. However, their use is limited due to the contents of bufadienolides, known cardiotoxins. This study aimed to establish a semi-quantitative profile of bufadienolides in the juices of K. blossfeldiana, K. daigremontiana, and K. pinnata using UHPLC combined with charged aerosol detection (CAD) and high-resolution mass spectrometry (HR-MS). Additionally, the study determined the ability of bufadienolides to penetrate the skin barrier using the Bronaugh Diffusion Cell Apparatus and Strat-M membrane. The study also assessed the ferric and molybdenum-reducing powers, as well as the radical scavenging capabilities of these plants juices using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) methods. The in vitro antihyaluronidase and antityrosinase activities and sun protection factor (SPF) were evaluated spectrophotometrically, indicating moderate capability to inhibit the skin enzymes, but low SPF protection for all analyzed juices. The semi-qualitative analysis demonstrated the presence of bufadienolides occurring in two juices from K. daigremontiana and K. pinnata, with the highest contents of 1,3,5-bersaldegenin-orthoacetate, bryophyllin-A/bryotoxin-C, bersaldegenin-acetate/bryophyllin-C, and diagremontianin. After passing through the skin model, no bufadienolide compounds were present in the subcutaneous filtrate. Antiradical and reduction assays revealed the antioxidant potential of K. blossfeldiana and K. pinnata. These results indicate that Kalanchoe juices have antiaging potential and appear safe for dermal applications.
Efficient Preparation of Li2FeSiO4/C with High Purity and Excellent Electrochemical Performance in Li-Ion Batteries
Authors: Cui Jinhai , Chen Dezhi , Xie Mengna , Zhou Yongheng , Dong Shuai , Wei Wei
Published: February 2025

One method to enhance the electrochemical performance of carbon-coated Li2FeSiO4 cathode material in lithium-ion batteries is to produce an ideal Li2FeSiO4 precursor with minimal impurities. A novel precursor for Li2FeSiO4 (Li2O·FeCO3·CH3OSiO2H) was synthesized through a methanol solvothermal reaction under stringent conditions (180 °C and 2.7 MPa), achieving a purity level of 93.2%. During synthesis, the new Li2FeSiO4 precursor exhibits unique self-purification properties and maintains a fine morphology after annealing. The resulting carbon-coated Li2FeSiO4 composites demonstrate a Brunauer–Emmett–Teller specific surface area of 102.4 m2/g and approximately 81% mesoporous volume, with 90% of the pore sizes measuring less than 39 nm. As a cathode material for lithium-ion batteries, this carbon-coated Li2FeSiO4 exhibits initial specific capacities of 172.3 mAh/g (charge) and 159.3 mAh/g (discharge). Remarkably, nearly 50% of the theoretical specific capacity remains after 1300 cycles at a rate of 0.1 C. The excellent electrochemical performance of the carbon-coated Li2FeSiO4 materials is demonstrated by their high lithium-ion diffusivity (DLi+) value of 1.26 × 10−11 cm2/s. Additionally, the enormous capacities-controlled diffusion contribution, which accounts for 70% of the total diffusion at a rate of 1C, is noteworthy. This performance can be attributed to the high purity of the carbon-free Li2FeSiO4 composite, which contains 91% Li2FeSiO4, as well as its favorable morphology.
Effects of Different Drying Methods on Volatile Flavor Compounds in Idesia Polycarpa Maxim Fruit and Oil
Authors: Ping Hongrui , Ge Yonghui , Liu Wenxuan , Yang Jinxiang , Zhong Zhaoxue , Wang Jinhua
Published: February 2025

This study combined gas chromatography–ion mobility spectrometry (GC-IMS) and multivariate statistical analysis to explore the differences in the characteristic aroma of Idesia polycarpa Maxim (I. polycarpa) fruit and oil under different drying methods: natural drying (ND), hot air drying (HAD), microwave drying (MD), and microwave vacuum drying (MVD). The results revealed that 91 volatile compounds were identified in the fruit, and 82 were found in the oil of I. polycarpa. HAD and MD resulted in the most significant loss of volatile aroma in both the fruit and oil. In contrast, MVD demonstrated the best retention of these volatile aromas. Multivariate statistical analysis and odor activity value (OAV) analysis (OAV ≥ 1) were employed to identify 10 volatile aroma compounds considered differentiating factors in the fruit and oil subjected to different drying methods. These compounds, including hexanal, 3-methylbutyric acid, 2-acetylpyridine, guaiacol, valeraldehyde, and butyric acid, significantly contribute to the flavor characteristics of I. polycarpa fruit and oil, evoking notes of nuts, caramel, and sourness. The OAVs of these aroma-differentiating compounds in microwave vacuum-dried fruit and oil were higher compared to those from other drying methods. Therefore, when considering the enhancement of volatile flavor compounds, MVD is more effective than the other drying methods in promoting the formation of flavor compounds in I. polycarpa fruit and oil.
The Anti-Aging Mechanism of Metformin: From Molecular Insights to Clinical Applications
Authors: Zhang Ting , Zhou Lijun , Makarczyk Meagan J. , Feng Peng , Zhang Jianying
Published: February 2025

Aging represents a complex biological phenomenon marked by the progressive deterioration of physiological functions over time, reduced resilience, and increased vulnerability to age-related diseases, ultimately culminating in mortality. Recent research has uncovered diverse molecular mechanisms through which metformin extends its benefits beyond glycemic control, presenting it as a promising intervention against aging. This review delves into the anti-aging properties of metformin, highlighting its role in mitochondrial energy modulation, activation of the AMPK-mTOR signaling pathway, stimulation of autophagy, and mitigation of inflammation linked to cellular aging. Furthermore, we discuss its influence on epigenetic modifications that underpin genomic stability and cellular homeostasis. Metformin’s potential in addressing age-associated disorders including metabolic, cardiovascular, and neurodegenerative diseases is also explored. The Targeting Aging with Metformin (TAME) trial aims to provide key evidence on its efficacy in delaying aging in humans. Despite these promising insights, significant challenges persist in gaining a more comprehensive understanding into its underlying mechanisms, determining optimal dosing strategies, and evaluating long-term safety in non-diabetic populations. Addressing these challenges is crucial to fully realizing metformin’s potential as an anti-aging therapeutic.
Exploration of Regulation of Alcohol Dehydrogenation Reaction of Dibenzimidazole-Based Ruthenium Complexes
Authors: Li Shuai , Jiang Min , Dekyvere Sander , Wang Peng , Chen Cheng , Verpoort Francis
Published: February 2025

This work demonstrates the synthesis of a series of Ru(II) complexes with dibenzimidazole-based ligands and investigates the spatial relationship between ligand structure and complexes. It explores the effect of substituent changes on the N-Ru-N bond angle of the complexes, as well as the distance from the Ru center to the aryl group, and utilizes these complexes to catalyze benzyl alcohol dehydrogenation in toluene. It was found that the ligand [Ru]-complex with a spatial configuration of electron donating groups had a larger N-Ru-N bond angle and higher catalytic performance at the ruthenium center, with a yield of 91.2%. Moreover, its distance from the umbrella hydrocarbon was sufficient to allow for the attack of the reactant molecules and the occurrence of catalytic reactions. The reaction mechanism was subsequently derived. This manuscript is expected to provide assistance and inspiration for the development of high-performance catalysts for alcohol dehydrogenation reactions.
Comparative Evaluation of Vasorelaxant and Antiplatelet Activity of Two Plant-Derived Benzoquinones: Rapanone and Embelin
Authors: Wróbel-Biedrawa Dagmara , Kubacka Monika , Kotańska Magdalena , Bednarski Marek , Grabowska Karolina , Podolak Irma
Published: February 2025

Vasorelaxant and antiplatelet agents play an important role in preventing and combating endothelial dysfunction, atherosclerosis and a plethora of associated cardiovascular diseases (CVDs). CVDs are the leading cause of death worldwide and nowadays occur not only in developed but also in developing societies. They include, among others, coronary heart disease, cerebrovascular disease and peripheral artery disease. Due to their high prevalence, it is important to seek efficient preventive measures, such as lifestyle changes and the implementation of appropriate herbal dietary supplementation and treatment alternatives. Plant-derived quinones have recently drawn researchers’ attention due to their interesting biological potential. Embelin and rapanone are two plant-derived benzoquinones with anti-inflammatory and antioxidant properties. Embelin has already been shown to have vasorelaxant and antiplatelet activity, but little is known about rapanone in the context of CVDs. Therefore, we decided to comparatively evaluate their activity in a specially designed experimental protocol. Following the isolation of both benzoquinones from plant sources (rapanone from Ardisia crenata leaves; embelin from Lysimachia punctata roots), their effects were comparatively assessed in a biofunctional study on isolated rat aorta (precontracted with phenylephrine) and in vitro on platelet aggregation. Both benzoquinones showed 50% vasorelaxation in an NO-dependent manner. Interestingly, rapanone was slightly more effective as an antiplatelet agent than embelin. The antiplatelet effect of both benzoquinones was specific, as no cytotoxicity towards platelets was observed at the concentrations tested. This is the first report on the vasorelaxant and antiplatelet activity of rapanone.
The Role of Brain-Derived Neurotrophic Factor as an Essential Mediator in Neuronal Functions and the Therapeutic Potential of Its Mimetics for Neuroprotection in Neurologic and Psychiatric Disorders
Authors: Numakawa Tadahiro , Kajihara Ryutaro
Published: February 2025

Among neurotrophins, including nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4/5), BDNF has been extensively studied for its physiological role in cell survival and synaptic regulation in the central nervous system’s (CNS’s) neurons. BDNF binds to TrkB (a tyrosine kinase) with high affinity, and the resulting downstream intracellular signaling cascades play crucial roles in determining cell fate, including neuronal differentiation and maturation of the CNS neurons. It has been well demonstrated that the downregulation/dysregulation of the BDNF/TrkB system is implicated in the pathogenesis of neurologic and psychiatric disorders, such as Alzheimer’s disease (AD) and depression. Interestingly, the effects of BDNF mimetic compounds including flavonoids, small molecules which can activate TrkB-mediated signaling, have been extensively investigated as potential therapeutic strategies for brain diseases, given that p75NTR, a common neurotrophin receptor, also contributes to cell death under a variety of pathological conditions such as neurodegeneration. Since the downregulation of the BDNF/TrkB system is associated with the pathophysiology of neurodegenerative diseases and psychiatric disorders, understanding how alterations in the BDNF/TrkB system contribute to disease progression could provide valuable insight for the prevention of these brain diseases. The present review shows recent advances in the molecular mechanisms underlying the BDNF/TrkB system in neuronal survival and plasticity, providing critical insights into the potential therapeutic impact of BDNF mimetics in the pathophysiology of brain diseases.
Untargeted Lipidomic Reveals Potential Biomarkers in Plasma Samples for the Discrimination of Patients Affected by Parkinson’s Disease
Authors: Tkachenko Kateryna , González-Sáiz Jose María , Pizarro Consuelo
Published: February 2025

Nowadays, the diagnosis of Parkinson’s disease (PD) remains essentially clinical, based on the subjective observations of clinicians. In addition, misdiagnosis with other neuro disorders, such as Alzheimer’s (AD), can occur. Herein, an untargeted lipidomic analysis of 75 plasma samples was performed to identify lipid species capable of discriminating between these two neuro groups. Therefore, PLS-DA and OPLS-DA analysis revealed significant differences in patient profiles in the sphingolipid and glycerophospholipid categories. As a result, a putative lipid biomarker panel was developed, which included HexCer (40:1; O2) and PC (O-32:0), with an area under the receiver operating characteristic curve (AUC) > 80, respectively. This panel was effective in discriminating between diseased and healthy subjects, but most importantly, it could discriminate between two neurodegenerative disorders that can present similar symptoms, namely PD and AD. Together, these findings suggest that the dysregulated metabolism of lipids plays a critical role in AD and PD pathology and may represent a valuable clinical tool for their diagnosis. Thus, further targeted studies are encouraged to better understand the underlying mechanisms of PD and confirm the diagnostic potency of the identified lipid metabolites.
Self-Supported Cu/Fe3O4 Hierarchical Nanosheets on Ni Foam for High-Efficiency Non-Enzymatic Glucose Sensing
Authors: Xu Jing , Cai Hairui , Yu Ke , Hou Jie , Li Zhuo , Zeng Xiaoxiao , He Huijie , Zhang Xiaojing , Su Di , Yang Shengchun
Published: February 2025

Electrochemical glucose sensors are vital for clinical diagnostics and the food industry, where accurate detection is essential. However, the limitations of glucose oxidase (GOx)-based sensors, such as complex preparation, high cost, and environmental sensitivity, highlight the need for non-enzymatic sensors that directly oxidize glucose at the electrode surface. In this study, a self-supporting hierarchical Cu/Fe3O4 nanosheet electrode was successfully fabricated by in situ growth on Ni Foam using a hydrothermal method, followed by annealing treatment. The Cu/Fe3O4 hierarchical nanosheet structure, with its large surface area, provides abundant active sites for electrocatalysis, while the strong interactions between Cu/Fe3O4 and Ni Foam enhance electron transfer efficiency. This novel electrode structure demonstrates exceptional electrochemical performance for non-enzymatic glucose sensing, with an ultrahigh sensitivity of 12.85 μA·μM−1·cm−2, a low detection limit of 0.71 μM, and a linear range extending up to 1 mM. Moreover, the Cu/Fe3O4/NF electrode exhibits excellent stability, a rapid response (~3 s), and good selectivity against interfering substances such as uric acid, ascorbic acid, H2O2, urea, and KCl. It also shows strong reliability in analyzing human serum samples. Therefore, Cu/Fe3O4/NF holds great promise as a non-enzymatic glucose sensor, and this work offers a valuable strategy for the design of advanced electrochemical electrodes.
The Impact of Long-Term Parenteral Nutrition on Physical Development and Bone Mineralization in Children with Chronic Intestinal Failure
Authors: Romanowska Hanna , Danko Mikołaj , Popińska Katarzyna , Żydak Joanna , Sibilska Marta , Wielopolska Joanna , Bartoszewicz Klaudia , Borkowska Anna , Walczak Mieczysław , Książyk Janusz
Published: February 2025

Background: This cross-sectional study aimed to assess growth, body weight, and bone mineralization and to identify predictors of metabolic bone disease (MBD) in children with chronic intestinal failure (CIF) on long-term parenteral nutrition (LPN). Methods: Twenty-six children with CIF were evaluated on total parenteral nutrition (PN) for at least three years, and 60 healthy controls were assessed. Measurements included body weight, height, BMI, serum levels of 25-hydroxyvitamin D3 (25-OHD3), calcium (Ca), phosphorus (P), magnesium (Mg), and aluminum (Al), as well as urinary excretion of these elements. Dual-energy X-ray absorptiometry (DXA) and the mid-arm muscle area (MAMA) and mid-arm fat area (MAFA) of the CIF group were estimated. Results: CIF children had significantly lower height, weight, and BMI Z-scores than controls (p < 0.001). While the median bone mineral density (BMD) Z-score was >−2, 34.7% had L1–L4 Z-scores ≤ −2. Urinary Ca and Al excretion were higher in LPN patients, positively correlating with serum 25-OHD3 levels (r = 0.48). Lower serum Ca, P, and Mg were observed in LPN patients (p < 0.001), and BMC L1–L4 correlated positively with MAMA, MAFA, and BMI. Conclusions: The physical development of children who require long-term parenteral nutrition due to intestinal failure is poorer than that of healthy children. Although there are risk factors for bone mineralization disorders in children with intestinal failure, no clinical issues, such as pathological fractures, have been observed.
A Preliminary Analysis of the Influence of Elderberry Juice Consumption on Thyroid Metabolism in Mice and Humans Fed High-Fat Diets
Authors: Jarrett Catherine L. , Teets Christy , Carbonero Franck G. , Etter Andrea J. , Solverson Patrick M.
Published: February 2025

Background/Objectives: Elderberry juice (EBJ) consumption prevents weight gain in mice fed a high-fat diet and increases fat oxidation in response to a meal challenge in overweight humans. Thyroid hormones influence metabolism and substrate oxidation, and the impact of EBJ consumption on thyroid homeostasis remains unexplored. Thus, the primary objective of this analysis was to investigate whether elderberry consumption in mice and humans affects serum thyroid biomarkers. Methods: Serum samples from a previous trial incorporating an EBJ intervention were analyzed for thyroxine (T4), thyroid stimulating hormone (TSH), and thyroglobulin (Tg). The samples are from a meal-tolerance test in 18 humans who followed a 7-day diet-controlled crossover design. Samples from 33 male mice were collected after 13 weeks following a high-fat diet with or without EBJ powder, and with or without free wheel running. Results: Short-term EBJ consumption in humans resulted in significant increases in T4 (PL: 42.3 ± 3.2 vs. EBJ: 49.3 ± 4.1 ng/mL, p < 0.05), and TSH (PL: 0.094 ± 0.012 vs. EBJ: 0.104 ± 0.011 ng/mL, p < 0.05), with no change in Tg (p > 0.05). Whereas supplementation with EBJ powder in rodents resulted in a non-significant reduction in T4 (p = 0.07). Conclusions: These findings suggest that elderberry juice consumption may influence thyroid metabolism, contributing to the observed metabolic benefits, such as improved fat oxidation, body composition, and protection against high-fat diet-induced weight gain. The increased T4 and TSH in humans align with enhanced metabolic rate, while the reduction in T4 in rodents indicates potential long-term adaptations requiring further exploration.
High Rates of Abnormal Glucose Metabolism Detected by 75 g Oral Glucose Tolerance Test in Major Psychiatric Patients with Normal HbA1c and Fasting Glucose Levels
Authors: Yoshida Sumiko , Aizawa Emiko , Ishihara Naoko , Hattori Kotaro , Segawa Kazuhiko , Kunugi Hiroshi
Published: February 2025

Objectives: Comorbid diabetes is an important factor in the treatment of major psychiatric disorders. However, a substantial proportion of diabetic patients remain undetected by routine diabetic indices such as blood glucose and HbA1c. This study tried to estimate rates of such unidentified diabetic or prediabetic patients by using a 75 g oral glucose tolerance test (OGTT). Methods: Participants in the test were 25 patients with major depressive disorder (MDD), 28 patients with bipolar disorder (BP), 26 patients with schizophrenia, and 28 psychiatrically normal controls. They were all Japanese, and showed non-diabetic levels of blood glucose (<126 mg/dL) and HbA1c (<6.0%). Results: Relatively high rates of psychiatric patients showed diabetes mellites (DM)-type abnormality (32% of MDD, 21.4% of BP and 42.3% of schizophrenia v. 10.7% of controls). The difference in the rates between schizophrenia and control groups was statistically significant (p = 0.008). When abnormal glucose metabolism was defined as a prediabetic state (either normal high glycemia, impaired fast glycemia or impaired glucose tolerance) or DM type in OGTT, it was more frequently seen in the psychiatric patients than in controls (64% of MDD, 46.4% of BP and 46.2% of schizophrenia v. 35.7% of controls). Individuals with DM type showed higher HbA1c values compared with those with normal (p < 0.001) and prediabetic (p = 0.021) states. Conclusions: The results suggest that relatively high proportions of patients with a major psychiatric disorder remain undetected by routine indices for abnormal glucose metabolism, indicating the importance of OGTT even if the patients showed non-diabetic levels in blood glucose or HbA1c.
Cholesin mRNA Expression in Human Intestinal, Liver, and Adipose Tissues
Authors: Gilliam-Vigh Hannah , Suppli Malte P. , Heimbürger Sebastian M. N. , Lund Asger B. , Knop Filip K. , Ellegaard Anne-Marie
Published: February 2025

Objective: Cholesin is a recently discovered gut-derived hormone secreted by enterocytes upon dietary cholesterol uptake via the transmembrane sterol transporter Niemann–Pick disease C1-like intracellular cholesterol transporter 1 (NPC1L1). In the liver, cholesin activates G protein-coupled receptor 146 (GPR146), causing reduced cholesterol synthesis. In this exploratory, hypothesis-generating study based on post hoc analysis, human data on the cholesin system are presented. Methods: Mucosal biopsies were collected throughout the intestinal tract from 12 individuals with type 2 diabetes (T2D) and 12 healthy, matched controls. Upper small intestinal mucosal biopsies were collected from 20 individuals before and after Roux-en-Y gastric bypass (RYGB) surgery. Liver biopsies were collected from 12 men with obesity and 15 matched controls without obesity. Subcutaneous abdominal adipose tissue biopsies were collected from 20 men with type 1 diabetes (T1D). All biopsies underwent full mRNA sequencing. Results: Cholesin mRNA expression was observed throughout the intestinal tracts of the individuals with T2D and the controls, in the livers of men with and without obesity, and in adipose tissue of men with T1D. NPC1L1 mRNA expression was robust throughout the small intestines but negligible in the large intestines of both individuals with and without T2D. RYGB surgery induced the expression of NPC1L1 mRNA in the upper small intestine. GPR146 mRNA was expressed in the livers of men, both with and without obesity, and in the adipose tissue of men with T1D, but not in the intestines. Conclusions: Our results suggest a role of the cholesin system in human physiology, but whether it is perturbed in metabolic diseases remains unknown. Clinical trial registration numbers: NCT03044860, NCT03093298, NCT02337660, NCT03734718.
The Importance of Nutriepigenetics in Older Adults with Inflammatory Bowel Disease
Authors: Prada Ana-Gabriela , Gîță Costina Daniela , Gîdei Sandra Monica , Istratescu Doina , Stroie Tudor , Aurelian Sorina Maria , Prada Gabriel Ioan , Penes Ovidiu Nicolae , Pop Anca Lucia , Diculescu Mihai Mircea
Published: February 2025

Background: Nutriepigenetics, the study of nutritional effects on gene expression, offers new insights into the clinical variability and relapse frequency of inflammatory bowel disease (IBD). As older adult populations are frequently unrepresented in the literature regarding their nutrition’s influence on their gene expression, we considered that studying this aspect would shed light on this population group. Materials and Methods: We conducted a prospective observational study on 60 IBD patients, categorized by age, residence, and nutrition access. Patients were grouped according to age groups (“young”-old—65–74 years old and old-old—75–86 years old), gender, IBD type, organic diet preference, Simple Clinical Colitis Activity Index (SCCAI), and Harvey-Bradshaw Index (HBI) at diagnosis and after diet change. All patients were followed up at the Fundeni Clinical Institute in Bucharest, Romania, and received personalized dietary recommendations, in accordance with the European guidelines. Results: Our findings revealed that a diet that was predominantly organic had a positive impact on decreasing the number of relapse episodes, irrespective of IBD type. Predominant diets, rich in dairy and saturated fats, mostly from non-organic sources, exacerbated inflammation and increased the frequencies of relapses. Conclusions: Older adult patients who adopted a predominantly organic diet had a reduction in frequency of relapse episodes, thus proving that diet is an important part of the treatment regimen of this population.
Interplay of n-3 Polyunsaturated Fatty Acids, Intestinal Inflammation, and Gut Microbiota in Celiac Disease Pathogenesis
Authors: Bascuñán Karla A. , Araya Magdalena , Rodríguez Juan Manuel , Roncoroni Leda , Elli Luca , Alvarez Josefina Del Pilar López , Valenzuela Rodrigo
Published: February 2025

Celiac disease (CD) is a chronic autoimmune disorder driven by both genetic and environmental factors, with the HLA DQ2/DQ8 genotypes playing a central role in its development. Despite the genetic predisposition, only a small percentage of individuals carrying these genotypes develop the disease. Gluten, a protein found in wheat, rye, and barley, is the primary environmental trigger, but other factors, such as the intestinal microbiota, may also contribute to disease progression. While the gluten-free diet (GFD) remains the cornerstone of treatment, many CD patients experience persistent inflammation and gut dysbiosis, leading to ongoing symptoms and complications. This chronic inflammation, which impairs nutrient absorption, increases the risk of malnutrition, anemia, and other autoimmune disorders. Recent studies have identified an altered gut microbiota in CD patients, both on and off the GFD, highlighting the potential role of the microbiota in disease pathogenesis. An emerging area of interest is the supplementation of n-3 polyunsaturated fatty acids (PUFAs), known for their anti-inflammatory properties, as a potential therapeutic strategy. n-3 PUFAs, found in fish oil and certain plant oils, modulate the immune cell function and cytokine production, making them a promising intervention for controlling chronic inflammation in CD. This review explores the current understanding of n-3 PUFAs’ effects on the gut microbiota’s composition and inflammation in CD, with the goal of identifying new avenues for complementary treatments to improve disease management.
Impact of Probiotic Supplementation and High-Intensity Interval Training on Primary Dysmenorrhea: A Double-Blind, Randomized Controlled Trial Investigating Inflammation and Hormonal Modulation
Authors: Yang Min-Yi , Chen Hao-Yu , Ho Chi-Hong , Huang Wen-Ching
Published: February 2025

Background: Dysmenorrhea, categorized as primary (PD) or secondary (SD), significantly affects females during their reproductive years, impairing quality of life, performance, and social relationships. Alongside medical treatment, exercise and probiotics are complementary measures for managing PD and promoting health. This study examined the impact of probiotic supplementation and high-intensity interval training (HIIT) on PD severity, physiological modulation, and physical fitness. Methods: Participants, recruited according to the primary dysmenorrhea criteria, were divided into non-PD (control) and PD groups, with the PD group further classified into dysmenorrhea (Dysmen), dysmenorrhea with probiotics (DysmenPro), dysmenorrhea with exercise (DysmenEx), and dysmenorrhea with both (DysmenExPro). Interventions included 10 weeks of HIIT and probiotics. Pre- and post-intervention assessments included questionnaires on premenstrual and menstrual symptoms, physical fitness evaluations, and blood sample analyses for biochemical, hormonal, and prostaglandin levels. Results: HIIT significantly reduced premenstrual symptoms, menstrual distress, and pain severity, likely due to hormone (estradiol, prolactin, progesterone, cortisol) modulation and decreased inflammation (high-sensitivity C-reactive protein, PGE2, PGF2α). Cardiovascular endurance and explosive strength showed improvement through high-intensity interval training (HIIT), whereas probiotics had no significant effect on these aspects of physical fitness. While probiotics reduced premenstrual and menstrual distress symptoms, they had no notable impact on pain, inflammation, or hormone levels. Dysmenorrhea-related discomforts were correlated significantly with inflammation and hormones. Conclusions: The intervention strategy involving probiotics and HIIT exercise may be utilized as an alternative and complementary treatment to alleviate PD symptoms. Furthermore, this strategy could also be incorporated into educational health plans to promote women’s health and potentially prevent gynecological disorders in the adolescent population.
Nutritional Interventions for Pressure Ulcer Prevention in Hip Fracture Patients: A Systematic Review and Meta-Analysis of Controlled Trials
Authors: Moran Jose M. , Trigo-Navarro Laura , Diestre-Morcillo Esther , Pastor-Ramon Elena , Puerto-Parejo Luis M.
Published: February 2025

Background/Objective: Pressure ulcers represent a significant complication in patients with reduced mobility, such as those recovering from hip fractures. In the present study, we aimed to comprehensively assess the impact of oral nutritional interventions on the development of pressure ulcers in hip fracture patients via a systematic review and meta-analysis of controlled studies evaluating the effectiveness of oral nutritional supplements compared with standard care. Methods: In accordance with PRISMA standards, this systematic review and meta-analysis of controlled studies evaluated the effectiveness of any type of oral nutritional supplements compared with standard care in hip fracture patients. The risk of bias was evaluated using the Cochrane ROB2 tool for randomized controlled trials and the ROBINS-1 tool for nonrandomized trials. Results: Fourteen studies (10 randomized controlled trials and 4 controlled trials) published since 1990 (n = 1648) were included. Oral nutritional supplementation was associated with a statistically significant decrease in the odds ratio of developing pressure ulcers in hip fracture patients (OR 0.54, 95% CI: 0.40–0.73, p < 0.001). Conclusions: The incidence and evolution of pressure ulcers can be improved by oral dietary supplementation in patients who have undergone hip fracture surgery. Accordingly, we propose that oral nutritional supplementation should be considered an essential component of comprehensive post-hip-fracture care.
Anticancer and Antioxidant Effects of Bioactive Peptides from Black Soldier Fly Larvae (Hermetia illucens)
Authors: Praseatsook Kwanchanok , Vachiraarunwong Arpamas , Taya Sirinya , Setthaya Phatthawin , Sato Kenji , Wanibuchi Hideki , Wongpoomchai Rawiwan , Dejkriengkraikul Pornngarm , Gi Min , Yodkeree Supachai
Published: February 2025

Background: Protein hydrolysates from insects are recognized for their biological activities. Black soldier fly larvae (BSFL) have drawn attention due to their antioxidant protein hydrolysates. However, research on bioactive peptides derived from these hydrolysates, particularly their cancer chemopreventive potential, remains limited. This study aims to evaluate the antioxidant, anti-inflammatory, antimutagenic, and anticancer activities of BSFL-derived bioactive peptides and explore the molecular mechanisms. Methods: Alkali-soluble BSFL protein (ASBP) was extracted and hydrolyzed using Alcalase and bromelain under optimized conditions. Antioxidant activity was assessed via FRAP, ABTS, and DPPH assays. The hydrolysate with the highest antioxidant activity was fractionated into molecular weight (MW) groups (>30, 10, and <3 kDa). The bioactivity of fractionated peptides was evaluated through antioxidant, anti-inflammatory (nitric oxide production in RAW 264.7 cells), antimutagenic (Ames test), and anticancer (CCK-8 assay on HCT 116, COLO205, Cw-2, and Caco-2 cells) assays. Mechanistic insights were obtained via microarray and Western blot analyses. Peptides were identified by LC-MS/MS. Results: The ASBP-Alcalase hydrolysate (ASBP-AH) showed optimal antioxidant activity at 3% (w/w) for 4 h. The ASBP-AH 30 (MW > 30 kDa) fraction exhibited the highest antioxidant capacity. In contrast, the ASBP-AH3 (MW < 3 kDa) fraction exhibited significant antimutagenic effects, reduced nitric oxide production, and decreased COLO205 cell viability. Treatment with ASBP-AH3 at its LC50 dose modulated the SKP2/p21/cyclin D1 pathways. Mostly peptides from ASBP-AH3 were composed of hydrophobic and charged amino acids. Conclusions: BSFL-derived bioactive peptides exhibit potential as multifunctional agents for cancer chemoprevention. In vivo studies are required to explore their clinical applications.
Body Fat and Visceral Fat Values in Spanish Healthcare Workers: Associated Variables
Authors: Tárraga Marcos Pedro Javier , López-González Ángel Arturo , Martínez-Almoyna Rifá Emilio , Paublini Oliveira Hernán , Martorell Sánchez Cristina , Tárraga López Pedro Juan , Ramírez-Manent José Ignacio
Published: February 2025

Background/Objectives: Excessive body adiposity is a significant public health challenge on a global scale. This study aimed to investigate the association between various sociodemographic factors and healthy lifestyle habits and the presence or absence of elevated body adiposity levels. Methodology: Two studies were conducted, a retrospective longitudinal study and a cross-sectional descriptive study. The analysis included 44,939 healthcare workers, categorised into four professional groups, to explore the relationship between age, sex, smoking, physical activity, and adherence to the Mediterranean diet and body adiposity, assessed as elevated body fat (BF) and visceral fat (VF) levels. Descriptive statistics encompassed categorical and quantitative variables, analysed using frequencies, Student’s t-tests, chi-square tests, and multinomial logistic regression models. Associations, concordances, and correlations were further examined using logistic regression and Cohen’s and Pearson’s kappa coefficients. Results: Age, sex, and physical activity were the factors most strongly associated with elevated BF and VF levels. Odds ratios (ORs) indicated the following significant associations: individuals aged 60 years and older exhibited ORs of 6.71 (95% CI: 5.68–7.74) for BF and 12.18 (95% CI: 10.01–14.26) for VF; male sex was associated with ORs of 2.21 (95% CI: 2.06–2.36) for BF and 12.51 (95% CI: 11.29–13.74) for VF. Sedentary behaviour was linked to ORs of 3.69 (95% CI: 3.41–3.97) for BF and 4.20 (95% CI: 3.78–4.63) for VF. Among healthcare professionals, nursing assistants and orderlies demonstrated the highest levels of adipose tissue accumulation. Conclusions: Elevated BF and VF levels among healthcare personnel are significantly associated by lifestyle factors, sex, and age, with the most pronounced risk observed in nursing assistants and orderlies. Further research focusing on the causal relationships between lifestyle behaviours and adiposity in this population will provide valuable insights and support the design of targeted preventive strategies to mitigate its prevalence.
Each Indicator of Socioeconomic Status (Education, Occupation, Income, and Household Size) Is Differently Associated with Children’s Diets: Results from a Cross-Sectional CroCOSI Study
Authors: Hasanović Jasmina , Križan Helena , Šatalić Zvonimir , Milanović Sanja Musić
Published: February 2025

Background: There has yet to be an agreement on which specific socioeconomic status (SES) indicator most effectively reflects disparities in children’s diets. However, children from lower SES backgrounds are particularly vulnerable, as research in other countries indicates that their diets contain fewer fruits and vegetables and more sweetened beverages. This paper aims to evaluate the associations between dietary habits and various SES indicators (education, occupation, income, and household size) among a representative sample of children in Croatia aged 7–10. Methods: Parents of children were asked to complete a questionnaire that contained indicators of their children’s dietary habits and socioeconomic status (n = 5608). Associations between SES and children’s dietary habits were assessed using logistic regression models. Results: The mother and father’s educational attainment were strongly positively associated with breakfast consumption. Children of parents with a lower educational level consumed sweetened beverages, sweet snacks, and fast food slightly more often than children in families with a higher educational background. The mother’s education was inversely associated with vegetable and cereal consumption, while the father’s education was inversely associated with fruit and bakery product consumption. Meanwhile, household income per unit had a significant influence on the consumption of soft drinks and bakery products. Household size had a significant influence solely on sweet snack consumption. Conclusions: Each SES indicator showed an independent association with at least one particular dietary habit, except for the parent’s employment status.
Diet Quality and Nutritional Assessment of Menus Served in Spanish Defense Ministry Preschool Canteens
Authors: Lozano Diego , Abenoza María , Bayarri Susana , Lázaro Regina
Published: February 2025

Background/Objectives: A healthy diet in early childhood has been shown to be one of the essential mainstays for the development of children. Few studies have been conducted on preschool canteens, despite the fact that they play a crucial role. Our study aimed to assess the dietary and nutritional quality of the menus served in Spanish Defense Ministry preschool education centers (1–3 years old) and to ascertain their compliance with dietary and nutritional recommendations. We also evaluated the influence of the canteen management model. Methods: Our cross-sectional observational study was carried out on the 448 menus offered in the 25 centers (operating under two management models: direct and contracted) of the Spanish Ministry of Defense during a school year all across the Spanish territory. Results: Under the contracted management model, the frequency of rice was low, as well as that of salads, eggs, fish, and fruit. The rotation within the fish group did not comply with recommendations, showing a shortage of oily fish. The menus’ energy values were correct, but the energy profile in the contracted management menus was higher than recommended in protein and fat while being lower in carbohydrates. The lipid profile was adequate. Values were high in cholesterol, simple sugars, fiber, vitamin K, selenium, potassium, and sodium; they were deficient in omega-3 fatty acids, vitamin D, iodine, and zinc. Conclusions: These results could help to establish special references or limits for the Spanish preschool population.