bluuebunny/crossref_metadata_2025 huggingface dataset:
Mapeamento dos protocolos disponíveis para comunicação de más notícias em saúde: protocolo de revisão de escopo
Authors: Cavalcante Fabíola Pinheiro Gomes , Cestari Virna Ribeiro Feitosa , Silva Anamaria Cavalcante e
Published: September 2022

Objetivo: mapear os protocolos disponíveis para comunicação de más notícias em saúde. Método: O protocolo será baseado nas diretrizes do Joanna Briggs Institute e a seleção dos estudos seguirá os direcionamentos propostos no Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews. Para disponibilidade científica, o protocolo foi registrado na plataforma Open Science Framework nº osf.io/sgev2. Para escopo da revisão serão consideradas as literaturas com evidências para resposta da pergunta norteadora: Quais os protocolos para comunicação de más noticiais disponíveis aos profissionais de saúde?. Os bancos de dados utilizados serão: PubMed/MEDLINE, Biblioteca Virtual em Saúde, Cochrane, Embase, Web of Science e Portal de Periódicos da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior. Como literatura cinzenta serão adotados: google scholar, a Biblioteca Digital Brasileira de Teses e Dissertações e Open Grey. A pesquisa ocorrerá de forma sistemática, aos pares e de forma independente. O gerenciador para arquivo e exclusão de artigos em duplicidade será o EndNoteWeb. As referências selecionadas serão enviadas ao software Rayyan- Intelligent Sistematic Review para a leitura de títulos e resumos e classificação utilizando os critérios de inclusão e exclusão. Em caso de divergência um terceiro revisor poderá intervir. Após tiragem, os estudos serão lidos na integra e novamente avaliados segundo os critérios pre-definidos. Os dados serão apresentados de forma a proporcionar ao leitor a visão robusta e atualizada sobre a disponibilidade de protocolos para comunicação de más notícias em saúde. Na discussão, aspectos relevantes serão apontados e interrelacionados de forma narrativa.
Saúde mental na Atenção Básica: perspectivas a partir da clínica ampliada e compartilhada
Authors: Martinello Eduarda Caroline Ceriolli , Fonsêca Graciela Soares
Published: September 2022

Objetivo: compreender a organização do cuidado para as demandas de saúde mental, focando o sofrimento psíquico, em uma Unidade Básica de Saúde (UBS) de um município de pequeno porte do Estado de Santa Catarina. Métodos: foram coletados dados por meio de grupos focais, audiogravados, com 3 equipes de Estratégias de Saúde da Família de uma UBS situada no referido município. Os dados foram tratados por meio da Análise de Conteúdo de Bardin e os resultados condensados em uma categoria. Resultados: verificou-se que a saúde mental na UBS analisada está intimamente relacionada com patologias e diagnósticos em detrimento do sujeito e sua singularidade, valorizando ações de cuidado pautadas na queixa conduta e que tenham como resultados a remissão dos sintomas a partir do enquadre da saúde mental em um ideal de normalidade. O médico é tido como centro da clínica em detrimento do trabalho interprofissional, o que protagoniza o uso de tecnologias duras e leve duras de cuidado, como medicações e diagnósticos, desconsiderando o uso das tecnologias leves como o acolhimento, a escuta e o vínculo. Considerações finais: Assim, verifica-se que as ações de cuidado em saúde mental na UBS analisada estão aliadas aos parâmetros de uma clínica tradicional.
Trilha inclusiva da consciência ecológica
Authors: Comis Amonega de Fátima , Venturini Bárbara , Prado Gilaine Santos , Berg Jéssica , Souza Nairana Catiele de , Schernn Cátia Roberta de Souza , Silva Jonas Cegelka da , Brodt Michele Santa Catarina
Published: September 2022

A licenciatura em Ciências Biológicas é desafiadora e ao mesmo tempo enriquecedora, permite constatar que o ensino de ciências possui conteúdos que podem e devem ser explorados das mais variadas formas e recursos metodológicos na busca de uma aprendizagem igualitária. Nessa perspectiva, este trabalho teve por objetivo desenvolver um material didático para ensino inclusivo de ciências, tendo por foco a deficiência intelectual (DI), com o tema ecologia. Embasados em referencial teórico sobre o assunto, que sugere como devem ser as didáticas pertinentes à aprendizagem de pessoas com DI, desenvolveu-se uma Trilha Ecológica em sala de aula. Esta foi desenvolvida em uma turma de sétimo ano do ensino fundamental, de uma escola pública de Santa Rosa/RS, a qual tinha uma aluna com laudo desta deficiência. A atividade teve ótima participação dos alunos e atingiu os objetivos esperados, que eram desenvolver uma atividade inclusiva que conduzisse os alunos à reflexão sobre o quanto o ser humano interfere no meio ambiente e quais são as possíveis mudanças de atitude que podemos ter para minimizar esses impactos. Este trabalho também permitiu conhecer como o ensino inclusivo é entendido por alguns professores, mediante aplicação e análise de questionário.
Biomonitorated study of Euphorbia umbellata (Pax) Bruyns extracts to be used to prepare silver nanoparticles with antimicrobial and cytotoxic potential
Authors: Rosa Rosana Leticia , Campos Patricia Mazureki , Cruz Luiza Stolz , Novak Robson Schimandeiro , Kanunfre Carla Cristine , Esmerino Luis Antônio , Schebelski Diego José , Paula Josiane de Fátima Padilha de , Beltrame Flavio Luís
Published: September 2022

This study proposes to develop a biomonitorated study of the Euphorbia umbellata leaves extracts to identify those that could be used to prepare, by green synthesis, silver nanoparticles (AgNPs), with cytotoxic and antimicrobial potential. Hexane, dichloromethane, acetone, methanol, and aqueous extracts were evaluated to measure the terpenes amounts, antimicrobial activity (Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus), and the cytotoxic potential (A549, H292 cell lines). Characterization of AgNPs was performed by determination of zeta potential, polydispersity index, mean diameter, UV/Vis spectrometry, and scanning electron microscope (SEM), and evaluated against cytotoxic and antimicrobial models. Hexane and dichloromethane extracts presented the higher amounts of terpenes, exhibited microbiological inhibition against K. pneumoniae and S. aureus, and cytotoxic potential against both cell lines. AgNPs prepared with hexane and dichloromethane extracts were determined after 35 minutes of artificial light exposure and presented spherical form with aggregates, mean diameter of 177.9 ± 1.3 nm, 288.8 ± 1.4 nm, polydispersity index of 0.416, 0.496, and zeta potential -35.7, -30.5 mV, respectively. AgNPs obtained from dichloromethane extract incremented in the biological activities, with IC50 of 1.56 µg/mL and 0.25 µg/mL against K. pneumoniae and S. aureus, respectively, and IC50 of 0.28 µg/mL for A549 and 0.18 µg/mL for H292 cell lines. The development of AgNPs with dichloromethane extract of E. umbellata using green synthesis demonstrated high potential to be used as cytotoxic and antimicrobial product, associating the phytochemical composition and the presence of AgNPs in a synergic effect.
POTENTIAL SITE SUITABILITY ANALYSIS OF SMALL HYDROPOWER PLANT IN IRANG RIVER CATCHMENT, MANIPUR, INDIA: A GEO-SPATIAL AND MCDM APPROACH
Authors:   The Assam Royal Global University, Assam, India-781035, Tongbram Purnachandra , Upadhyaya Chandra ,   The Assam Royal Global University, Assam, India
Published: September 2022

India being one of the leading developing country where 80% of its energy or power supply are produced by fossil fuel: coal, oil and solid biomass. Using of fossil fuel-based power led to environmental degradation and to reduce it an alternative power source is required. Amongst the alternate source, Hydropower is the most suitable source for power supply. Hydropower is one of the oldest renewable power sources where it generates power from the flow of water by converting its gravitational flow or potential energy of water source into electricity. Due to roughness and inaccessible of terrain of the study area, the use of conventional method becomes almost impossible. With the help of new innovative methodologies and techniques of Remote Sensing (RS) and Geographic Information System (GIS) this study has been carried out. In this study nine geo-spatial inputs are used to find the site suitable for small hydropower (SHP) project. The multi criteria decision making (MCDM) techniques is used to evaluate the input’s weights. After the weight is obtained for each input, it is overlaid according to its weight using overlay tool to obtained the site suitable for SHP project. The outcome of this study allows spotting identification of SHP potential zone
LUNG SCANS SEGMENTATION USING MARKER-CONTROLLED WATERSHED TRANSFORMATION
Authors:   UBDTCE, Davanagere, Karnataka, India, D Rajeshwari , Naika C. L, Dr. Shrinivasa ,   UBDTCE, Davanagere, Karnataka, India, Rafi Dr. Mohamed ,   UBDTCE, Davanagere, Karnataka, India
Published: September 2022

Image segmentation is the process of partitioning a digital image into multiple segments knows as set of pixels. Image segmentation is typically used to locate objects and boundaries (lines, curves, etc.) in images. It is the process of assigning a label to every pixel in an image such that pixels with the same label share certain visual characteristics. The Watershed Transform consists of an image partitioning into its constitutive regions. This transform is easily adapted to be used in different types of images and it allows distinguishing complex objects. The marker watershed transformation is an improvement one on the basis of the watershed transform, which is set a tag in the image. The tag can be a point, a line or an area, the importance is its location instead of the marked shape. Each tag represents the image of a final partition and selecting a tag is a key factor in the decision segmentation.
KITE SATELLITE
Authors:   Vidyalankar Institute of Technology, Mumbai, Kumari Neha , Ranghar Sharad , Mohota Smriti , Chavan Tapish ,   Vidyalankar Institute of Technology, Mumbai
Published: September 2022

These days a lot of modern weather monitoring systems are available for weather monitoring. These systems are executed on a very grand scale for monitoring real-time weather for a full city or nation. Implementing such a system for a small area is not feasible since they are not designed for it and the overhead cost for maintaining such systems for a small area is very high. Our proposed system makes use of multiple sensors to measure the weather or environmental parameters with the help of a Box Kite at a certain height. The values read from the sensors are processed by the Arduino micro-controller and then it sends the parameters to the base station using IoT techniques. The readings uploaded on a web server and are also displayed onboard LCD for quick viewing. All these readings will provide the weather characteristics of a particular area and record the weather pattern
DEEPFAKE DETECTION USING EYE-BLINKING PATTERN
Authors: SanilM Rithvika , Saathvik S. , RaiK Rithesh , P M Srinivas ,   Sahyadri College of Engineering & Management, Adyar
Published: September 2022

Deep learning algorithms have become so potent due to increased computing power that it is now relatively easy to produce human-like synthetic videos, sometimes known as & quot; deep fakes. & quot; It is simple to imagine scenarios in which these realistic face switched deep fakes are used to extort individuals, foment political unrest, and stage fake terrorist attacks. This paper provides a deep learning strategy novel for the efficient separation of fraudulent films produced by AI from actual ones. Automatically spotting replacement and recreation deep fakes is possible with our technology. To combat artificial intelligence, we are attempting to deploy artificial intelligence. The framelevel characteristics are extracted by our system using a Res-Next Convolution neural network, and later these features are applied to train an LSTM-based recurrent neural network to determine if those submitted video is being altered in any way or not, i.e. whether it is a deep fake or authentic video. We test our technique on a sizable quantity of balanced and mixed data sets created by combining the different accessible data sets, such as Face-Forensic++[1], Deep fake detection challenge[2], and Celeb-DF[3], in order to simulate real-time events and improve the model's performance on real-time data.
SOCIAL DISTANCE DETECTION
Authors: Abraham Shiji , Aathmaram Aanand , Krishna D Arjun , Munees Ahmmed , Nowfal Mahammad ,   Sahyadri college of engineering and management Mangalore Karnataka, India
Published: September 2022

COVID-19 is an illness caused by the SARSCoV-2 virus. The majority of COVID-19 patients will have low to normal symptoms and will recover without requiring extra care. Otherwise, some patients will become extremely sick and require medical support. A Deep Learning model is made to track people and measure the distance between them. To prevent the transmission of the infection, making people maintain the minimum distance is the key objective. We apply YOLOv3 and other convolutional neural network-based methods, as well as a distance measurement method, to recognise individuals. This Deep Learning model can determine whether members of a large group maintain their distance from one another. If they maintain a 6 feet distance, it will be indicated in green; if not, it will be highlighted in red. This can help identify social disparities and lessen the spread of COVID-19
INTERFACING OF ROBOTS FOR MANUFACTURING AUTOMATION
Authors: Srihari P , Sreeramulu D , Rao A. Raghupathi , Durgacharan N , Rao Ch. Bhaskara ,   Aditya Institute of Technology and Management (A), AITAM, Tekkali, India
Published: September 2022

This article outlines the various stages of operations involved in pick and place operation by the interfacing of 2 robots. The interfacing involves sending the input signals to the second robot from the first robot to perform the task. What coded by the user set of instructions. While performing any robot operation, a robot may interact with other robot in various 4 degrees ranging in between 2 extremes. Now-a-days, various advanced robots are used in industries, but still controlling is done manually or using processors likewise Arduino, microcontroller. Here the programmable logic and Controller is used for controlling and operating robotic arm. All the various problems of this process have been analyzed properly, and have been taken into consideration while programming and designing the pick and place robotic arm. Kinematics have been defined in DMU module of CATIA and Robot joints are simulated to validate the real time movements of physical robots (Igus Robotic control) of vacuum gripper and magnetic gripper igus robots present in the ARC Lab of Mechanical branch, Aditya Institute of Technology And Management. Initially, the whole layout of the manufacturing automation should be placed at correct coordinates i.e., assigning the robots, material handling and storage retrieval system to their respective coordinates. Designing of two different robots’ parts in CATIA V5 software and assembling the parts to form the vacuum and magnetic robots. The kinematics motions of robots are assigned in CATIA V5 software that gives the revolute motions of each joint and had made the simulations of two robots.
LOW POWER ELECTRIC TWO-WHEELER WITH INTELLIGENT COOLING TECHNOLOGY
Authors: Jose Thekkan Meenu , PS Alkka , VS Ardra , TM Saji , . AwinShajan ,   Universal Engineering College, Thrissur, Kerala, India
Published: September 2022

In the past decade, the electric two-wheelers have been popular in many of the southeast Asian countries for urban drives. Presence of electric twowheeler is fastly growing in India too. Environmental problems promote the development of new generation EVs for transportation. Electric scooters are plug-in electric vehicles that runs on battery and can be recharged from any external sources of electricity. The important parts of electric scooter include a motor, battery pack, controller etc. Since electric scooters are not using an engine, it becomes an effective way of road transport as it does not cause any pollution. In future, electric two-wheelers maybe a replacement product for the engine driven vehicles, provided that safety, drive performance and cost issues are similar to that of actual engine EVs. In this paper, we have proposed a low power electric twowheeler with an intelligent cooling technology. A custom build portable Li ion battery pack is developed which gives power to the electric scooter. An intelligent cooling technology is applied to the battery pack using a thermostat module to cool the battery while overheating. A gear mechanism is implemented to reduce gear strain on the motor. The advantage of the model is that it does not cause any pollution, more energy efficient, smooth operation and depends wholly on electricity.
ROBUSTNESS OF NTC-ALGORITHM FOR MISFITS IN CASE OF AIRFLOW CALIBRATION
Authors:   Hochschule Darmstadt, Darmstadt Germany, Kettner Jan-Eric
Published: September 2022

Study with comparison of three-point airflowauto calibration algorithm for NTC-thermistors. The results show that Hoge-5 is the best implementation of the NTC as well as the most robust to unavoidable calibration errors.
THE EFFECTS OF RESISTANCE BAND EXERCISES ON SOME PERFORMANCE PARAMETERS IN YOUNG FOOTBALL PLAYERS
Authors: MOR Ahmet , KARAKAŞ Fatih , MOR Hakkı , YURTSEVEN Rıfat , YILMAZ Ali Kerim , ACAR Kürşat
Published: September 2022

The aim of this study was to investigate the acute effect of resistance band exercises on some performance parameters in young football players. Active and trained 16 male football players (age: 15.18 ± .40 years; height: 170.81 ± 7.21 cm; weight: 59.43 ± 8.61 kg; body mass index (BMI): 20.26 ± 1.60 kg/m2) voluntarily participated in this study. Subjects performed two different warmup protocols including jogging+dynamic stretching exercises and jogging+dynamic stretching+resistance band exercises on non-consecutive days. Following the warm-up protocols and then three minutes of passive recovery, subjects were tested on the balance test, reaction time test, vertical jump and anaerobic power test, Illinois agility test, 30-m sprint, and ball kicking speed. Data were checked for normality by using Shapiro-Wilk test. Comparison between groups was analyzed with paired sample t-test. Besides, Cohen’s d was utilized in calculation of effect size. Statistical analyses and interpretations of the data were accepted as p<0.05. In comparison of the balance, vertical jump, 30-m sprint, and Illinois agility tests results between two groups, resistance band exercises were found to have positive effects on performance in all tests. However, statistically significant differences were detected in balance, agility, and sprint tests (p<0.05). In conclusion, balance, vertical jump, 30-m sprint, and Illinois agility test performance parameters of football players improved following the resistance band exercises. Accordingly, it is recommended that coaches and athletes incorporate resistance band exercises into sport-specific warmup protocols, and resistance band exercises should be performed in pre-training warm up session for achieving high performance in football players.
HISTORY OF THE SCIENTIFIC RATIONALE OF THE "TELEMEDICINE" CONCEPT: PROFESSOR K.T. BIRD’S RESEARCH GROUP CONTRIBUTION
Authors:   Research and Practical Clinical Center for Diagnostics and Telemedicine Technologies of the Moscow Health Care Department, Moscow, Russian Federation, VLADZYMYRSKYY ANTON
Published: September 2022

Scientific development of the problems of using telecommunications for medical purposes covers the period from the second half of the 19th century to the present day. The progress of technical and methodological aspects proceeded in parallel. After decades of scientific knowledge’s evolution, in the 1970s the concept and the term «telemedicine» appeared. The purpose of the article is to reconstruct the history of the scientific rationale of the «telemedicine» concept and term, also as to study the activities of the scientific group that played a key role in this process. In 1968-1976 at the Massachusetts General Hospital (USA), a scientific group of Professor Kenneth Bird functioned; they carried out two scientific projects to create a «telemedicine network». The group made consistent research, which resulted in the substantiation the «telemedicine» as the distant medical care using telecommunication technologies; the corresponding term was introduce into scientific circulation. The necessity of an integrated approach is substantiated. «Telemedicine» is perceived as a set of electrical communications, diagnostic devices, and methods of translation and interpretation of medical data. The scientific group for the first time evaluated the accuracy of distant diagnostic decisions made using two-way television communication. The special design for this scientific study created. Now, it become a classic.
Broadcasting anti-media populism in the Philippines: YouTube influencers, networked political brokerage, and implications for governance
Authors: Soriano Cheryll Ruth , Gaw Marie Fatima
Published: September 2022

This article interrogates platform governance and accountability amid the growing role of influencers in constructing political discourses, and particularly, in the intermediation of anti-media ideological frames through their embeddedness in networked assemblages. We deploy the concept of ‘networked political brokerage’ to examine the socio-technical relations among influencers, the platform, and its users, and how this dynamic assemblage engages in the intermediation of anti-media populism. The study draws from a critical examination of the network and discursive tactics deployed by Filipino YouTube influencers who advance partisan political commentary and deceptive narratives to delegitimize mainstream media institutions through issue network analysis concerning the franchise denial and eventual shutdown of the Philippines’ oldest media network, ABS-CBN. We problematize how influencers engage platform affordances and cultures of use to enable, amplify, and fortify the brokering of their political agenda within a larger network of political actors, while eliding accountability. Through the mutually affirming relationship of content creators and the platform, networked hyper-politicized contents gain visibility and galvanize anti-media rhetoric. Ultimately, the article raises concerns on the social consequences of networked political brokerage and offers a framework for how governance and policy discussions can treat the functioning of such networked political influence.
Republicans for Labor: State-level Labor Power and Republican Senate Behavior
Authors: R. Daneri Danny
Published: September 2022

Despite a half-century of decline in membership and political influence, unions still play a prominent role in lobbying for congressional policy. For much of post-war history, policies around labor and employment have divided along partisan lines, seemingly confining labor’s influence to politicians in the Democratic Party. In this paper, we construct a dataset of labor and employment legislation from 1970 to 2020 to examine the influence of state-level union density on Republican Senators’ votes over the past half-century. We use a two-stage ordinary least squares model with a novel instrument to provide causal estimates of the effect of unionization on Senator voting behavior. The results suggest that the percentage of employed workers who are in unions in a state is strongly predictive of whether Republicans will defect in favor of labor policies supported primarily by Democrats.
Commutative Ring and Field on the Binomial Coefficients of Combinatorial Geometric Series
Authors: Annamalai Chinnaraji
Published: September 2022

This paper discusses an abelian group, ring, and field under addition and multiplication of the binomial coefficients in combinatorial geometric series. The coefficient for each term in combinatorial geometric series refers to a binomial coefficient. This idea can enable the scientific researchers to solve the real life problems.
Effects of University Teachers’ Emotional Labor on their Well-Being and Job Satisfaction
Authors: CHEN Kieranna , CHEN Chenin
Published: September 2022

Excellent teachers who provide excellent educational quality educate students with both skills and virtues. Teachers face various emotional problems; they not only work with labor, intellect, and affection, but also have to show appropriate emotions under increasing pressure to create a rational and warm organizational climate in schools. Teachers’ hard work is the emotional labor load. The university teachers in Bangkok, Thailand participated in this study. A total of 480 copies of the questionnaire were distributed. After removing invalid and incomplete ones, 437 copies were remained valid, with a response rate of 91%. The research results showed that a university that fails to meet the teachers’ satisfaction and expectations can result in teachers’ low well-being and job satisfaction as well as teaching inefficiency which seriously lowers the effectiveness of the university and university teachers are medium and high emotional labor workers who consume large amount of emotion and labor in their workplaces, therefore, they can easily show burnout, teaching apathy, and even physical and mental exhaustion under excessive use of emotions, and 3. a university leader who is willing to find a solution to the teachers’ emotional labor load can help them correctly manage their emotions to obtain a high level of well-being and job satisfaction. According to the results it can be discussed that the current study is expected to help in terms of increasing the number of emotion management courses and channels for releasing teachers’ emotional labor load, avoiding emotional dissonance, and promoting teacher efficiency.
(Ан)тропология фронтира: дороги и тропы в судьбах приамурских эвенков
FRONTIER ANTRAILPOLOGY: ON THE TRAILS AND ROADS IN THE LIVES OF AMUR EVENKS
Authors: Mikhalev M. S.
Published: September 2022

На основе сравнения современных судеб двух небольших эвенкийских поселений в Хабаровском крае и в Амурской области обосновывается тезис о том, что сохранность культуры коренного малочисленного народа во многом зависит от того, насколько хорошо ему удается сберечь свою собственную систему координат. Показывается, что в то время как тропы обладают способность сохранять историческую память народа и тем самым препятствуют его ассимиляции, современные магистральные дороги и другие объекты крупной транспортной инфраструктуры ускоряют эрозию национальной культуры, являясь причиной потери связи народа со средой своего обитания, и, в конечном итоге, его гибели. Утверждается, что в то время как с точки зрения экономики и государственного управления дороги видятся «неизбежным благом», антропологи должны уделить особое внимание изучению троп и вопросам их сохранности. В этой связи в статье вводится понятие и обосновывается актуальность появления (ан)тропологии как отдельной субдисциплины, изучающей роль троп и других объектов «народной инфраструктуры» в жизни коренных малочисленных народов.
Based on a comparison of the two Evenk settlements in Russia’s Khabarovsky Krai and Amurskaya Oblast, this paper argues that preservation of traditional culture by an ethnic minority group depends on how well it preserves its own systems of axes. It shows also that while trails that are capable of keeping national heritage alive and preventing minorities from assimilation, modern highways cause traditional culture to decline as they destroy their ties with the environment that may eventually lead to its extinction. It states that while both economists and governmental agencies view road construction as an “inevitable good”, anthropologists shall pay attention to the study of trails as repositories of traditional culture and to their preservation. To achieve this goal, this paper suggests developing Antrailpology as a subdicipline within social anthropology that would elaborate on the role of trails and other “folk infrastructure” in the life of minority ethnic groups.
Детское (и) советское: взрослые и дети в музеях советской повседневности ADULTS AND CHILDREN IN MUSEUMS OF SOVIET EVERYDAY LIFE
Authors: Kupriyanov P. S.
Published: September 2022

В современных музеях советской повседневности 1950–1980 гг. тема детства занимает весьма важное место. Она присутствует как в музейном «сообщении» (и в экспозициях, и в экскурсиях), так и в его восприятии посетителями. Разговор о советском в музее почти никогда не обходится без детского компонента, а иногда практически сводится к нему. В статье рассматриваются формы, контексты и эффекты такого «альянса». Она основана на полевых материалах 2019–2021 гг., полученных в г. Коломне Московской области и в некоторых других российских городах, а также на данных с туристических и музейных сайтов и страниц в соцсетях. Анализируя избранные кейсы, автор отмечает, что для представителей старшего поколения детство служит важной формой или рамкой для ностальгического восприятия советского прошлого, и прослеживает механизмы, обеспечивающие эту функцию. Детское же восприятие рассмотренных экспозиций, особенно в популярном интерактивном формате варьирует между двумя основными моделями — реконструкцией (строящейся на экзотизации советского) и ресайклинга (предполагающего вторичное использование советских практик и ценностей). В статье отмечается недостаток данных, касающихся собственно детского музейного опыта, приводится исследовательский контекст рассматриваемой темы: актуальные направления и дискуссии, продуктивные теоретические концепты. The topic of childhood has an important place in contemporary museums of lateSoviet everyday life (1950–1980s). It can be seen in the message a museum conveys (both in what is shown and what is said) and in its perception by visitors. The topic of the Soviet in a museum nearly always includes the “children”’s component, and sometimes is virtually reduced to it. This paper examines the forms, contexts, and effects of such an “alliance”. The paper is based on the field material obtained in Kolomna, Moscow region, and in some other Russian cities in 2019–2021, and on the data from tourist and museum websites and social network accounts. Analyzing the selected cases, the author observes that the notion of childhood serves as an important frame for the nostalgic perception of the Soviet past for the older generation, and also demonstrates the mechanisms that fulfil this function. Children’s perception of expositions varies between two main models — reconstruction (based on the exoticization of the Soviet) and recycling (involving the reuse of Soviet practices and values). The article points out the lack of data concerning actual children’s museum experience and provides the research context for the topic: current trends, discussions and useful theoretical concepts.
Machine learning identifies exosome features related to hepatocellular carcinoma
Authors: Zhu Kai , Tao Qiqi , Yan Jiatao , Lang Zhichao , Li Xinmiao , Li Yifei , Fan Congcong , Yu Zhengping
Published: September 2022

Background: Hepatocellular carcinoma (HCC) is one of the most malignant tumors with a poor prognosis. There is still a lack of effective biomarkers to predict its prognosis. Exosomes participate in intercellular communication and play an important role in the development and progression of cancers.

Methods: In this study, two machine learning methods (univariate feature selection and random forest (RF) algorithm) were used to select 13 exosome-related genes (ERGs) and construct an ERG signature. Based on the ERG signature score and ERG signature-related pathway score, a novel RF signature was generated. The expression of BSG and SFN, members of 13 ERGs, was examined using real-time quantitative polymerase chain reaction and immunohistochemistry. Finally, the effects of the inhibition of BSG and SFN on cell proliferation were examined using the cell counting kit-8 (CCK-8) assays.

Results: The ERG signature had a good predictive performance, and the ERG score was determined as an independent predictor of HCC overall survival. Our RF signature showed an excellent prognostic ability with the area under the curve (AUC) of 0.845 at 1 year, 0.811 at 2 years, and 0.801 at 3 years in TCGA, which was better than the ERG signature. Notably, the RF signature had a good performance in the prediction of HCC prognosis in patients with the high exosome score and high NK score. Enhanced BSG and SFN levels were found in HCC tissues compared with adjacent normal tissues. The inhibition of BSG and SFN suppressed cell proliferation in Huh7 cells.

Conclusion: The RF signature can accurately predict prognosis of HCC patients and has potential clinical value.
The Flp type IV pilus operon of Mycobacterium tuberculosis is expressed upon interaction with macrophages and alveolar epithelial cells
Authors: Alteri Christopher J. , Rios-Sarabia Nora , De la Cruz Miguel A. , González-y-Merchand Jorge A. , Soria-Bustos Jorge , Maldonado-Bernal Carmen , Cedillo María L. , Yáñez-Santos Jorge A. , Martínez-Laguna Ygnacio , Torres Javier , Friedman Richard L. , Girón Jorge A. , Ares Miguel A.
Published: September 2022

The genome of Mycobacterium tuberculosis (Mtb) harbors the genetic machinery for assembly of the Fimbrial low-molecular-weight protein (Flp) type IV pilus. Presumably, the Flp pilus is essential for pathogenesis. However, it remains unclear whether the pili genes are transcribed in culture or during infection of host cells. This study aimed to shed light on the expression of the Flp pili-assembly genes (tadZ, tadA, tadB, tadC, flp, tadE, and tadF) in Mtb growing under different growth conditions (exponential phase, stationary phase, and dormancy NRP1 and NRP2 phases induced by hypoxia), during biofilm formation, and in contact with macrophages and alveolar epithelial cells. We found that expression of tad/flp genes was significantly higher in the stationary phase than in exponential or NRP1 or NRP2 phases suggesting that the bacteria do not require type IV pili during dormancy. Elevated gene expression levels were recorded when the bacilli were in contact for 4 h with macrophages or epithelial cells, compared to mycobacteria propagated alone in the cultured medium. An antibody raised against a 12-mer peptide derived from the Flp pilin subunit detected the presence of Flp pili on intra- and extracellular bacteria infecting eukaryotic cells. Altogether, these are compelling data showing that the Flp pili genes are expressed during the interaction of Mtb with host cells and highlight a role for Flp pili in colonization and invasion of the host, subsequently promoting bacterial survival during dormancy.
Unbiased screen for pathogens in human paraffin-embedded tissue samples by whole genome sequencing and metagenomics
Authors: Nienhold Ronny , Mensah Nadine , Frank Angela , Graber Anne , Koike Jacqueline , Schwab Nathalie , Hernach Claudia , Zsikla Veronika , Willi Niels , Cathomas Gieri , Hamelin Baptiste , Graf Susanne , Junt Tobias , Mertz Kirsten D.
Published: September 2022

Identification of bacterial pathogens in formalin fixed, paraffin embedded (FFPE) tissue samples is limited to targeted and resource-intensive methods such as sequential PCR analyses. To enable unbiased screening for pathogens in FFPE tissue samples, we established a whole genome sequencing (WGS) method that combines shotgun sequencing and metagenomics for taxonomic identification of bacterial pathogens after subtraction of human genomic reads. To validate the assay, we analyzed more than 100 samples of known composition as well as FFPE lung autopsy tissues with and without histological signs of infections. Metagenomics analysis confirmed the pathogenic species that were previously identified by species-specific PCR in 62% of samples, showing that metagenomics is less sensitive than species-specific PCR. On the other hand, metagenomics analysis identified pathogens in samples, which had been tested negative for multiple common microorganisms and showed histological signs of infection. This highlights the ability of this assay to screen for unknown pathogens and detect multi-microbial infections which is not possible by histomorphology and species-specific PCR alone.
Loss of Vhl alters trabecular bone loss during S. aureus osteomyelitis in a cell-specific manner
Authors: Ford Caleb A. , Hurford Ian M. , Fulbright Laura E. , Curry Jacob M. , Peek Christopher T. , Spoonmore Thomas J. , Cruz Victorio Virginia , Johnson Joshua R. , Peck Sun H. , Cassat James E.
Published: September 2022

Osteomyelitis, or bone infection, is a major complication of accidental trauma or surgical procedures involving the musculoskeletal system. Staphylococcus aureus is the most frequently isolated pathogen in osteomyelitis and triggers significant bone loss. Hypoxia-inducible factor (HIF) signaling has been implicated in antibacterial immune responses as well as bone development and repair. In this study, the impact of bone cell HIF signaling on antibacterial responses and pathologic changes in bone architecture was explored using genetic models with knockout of either Hif1a or a negative regulator of HIF-1α, Vhl. Deletion of Hif1a in osteoblast-lineage cells via Osx-Cre (Hif1aΔOB) had no impact on bacterial clearance or pathologic changes in bone architecture in a model of post-traumatic osteomyelitis. Knockout of Vhl in osteoblast-lineage cells via Osx-Cre (VhlΔOB) caused expected increases in trabecular bone volume per total volume (BV/TV) at baseline and, intriguingly, did not exhibit an infection-mediated decline in trabecular BV/TV, unlike control mice. Despite this phenotype, bacterial burdens were not affected by loss of Vhl. In vitro studies demonstrated that transcriptional regulation of the osteoclastogenic cytokine receptor activator of NF-κB ligand (RANKL) and its inhibitor osteoprotegerin (OPG) is altered in osteoblast-lineage cells with knockout of Vhl. After observing no impact on bacterial clearance with osteoblast-lineage conditional knockouts, a LysM-Cre model was used to generate Hif1aΔMyeloid and VhlΔMyeloid mouse models to explore the impact of myeloid cell HIF signaling. In both Hif1aΔMyeloid and VhlΔMyeloid models, bacterial clearance was not impacted. Moreover, minimal impacts on bone architecture were observed. Thus, skeletal HIF signaling was not found to impact bacterial clearance in our mouse model of post-traumatic osteomyelitis, but Vhl deletion in the osteoblast lineage was found to limit infection-mediated trabecular bone loss, possibly via altered regulation of RANKL-OPG gene transcription.
Therapeutic prospects of ceRNAs in COVID-19
Authors: Liu Lin , Zhang Yao , Chen Yu , Zhao Yueshui , Shen Jing , Wu Xu , Li Mingxing , Chen Meijuan , Li Xiaobing , Sun Yuhong , Gu Li , Li Wanping , Wang Fang , Yao Lei , Zhang Zhuo , Xiao Zhangang , Du Fukuan
Published: September 2022

Since the end of 2019, COVID-19 caused by SARS-CoV-2 has spread worldwide, and the understanding of the new coronavirus is in a preliminary stage. Currently, immunotherapy, cell therapy, antiviral therapy, and Chinese herbal medicine have been applied in the clinical treatment of the new coronavirus; however, more efficient and safe drugs to control the progress of the new coronavirus are needed. Long noncoding RNAs (lncRNAs), microRNAs (miRNAs), and circular RNAs (circRNAs) may provide new therapeutic targets for novel coronavirus treatments. The first aim of this paper is to review research progress on COVID-19 in the respiratory, immune, digestive, circulatory, urinary, reproductive, and nervous systems. The second aim is to review the body systems and potential therapeutic targets of lncRNAs, miRNAs, and circRNAs in patients with COVID-19. The current research on competing endogenous RNA (ceRNA) (lncRNA-miRNA-mRNA and circRNA-miRNA-mRNA) in SARS-CoV-2 is summarized. Finally, we predict the possible therapeutic targets of four lncRNAs, MALAT1, NEAT1, TUG1, and GAS5, in COVID-19. Importantly, the role of PTEN gene in the ceRNA network predicted by lncRNA MALAT1 and lncRNA TUG1 may help in the discovery and clinical treatment of effective drugs for COVID-19.
Coronary artery disease risk factors affected by RNA modification-related genetic variants
Authors: Li Ru , Zhang Huan , Tang Fan , Duan Chengcheng , Liu Dan , Wu Naqiong , Zhang Yonghong , Wang Laiyuan , Mo Xingbo
Published: September 2022

Background

Single nucleotide polymorphisms that affect RNA modification (RNAm-SNPs) may have functional roles in coronary artery disease (CAD). The aim of this study was to identify RNAm-SNPs in CAD susceptibility loci and highlight potential risk factors.

Methods

CAD-associated RNAm-SNPs were identified in the CARDIoGRAMplusC4D and UK Biobank genome-wide association studies. Gene expression and circulating protein levels affected by the RNAm-SNPs were identified by QTL analyses. Cell experiments and Mendelian randomization (MR) methods were applied to test whether the gene expression levels were associated with CAD.

Results

We identified 81 RNAm-SNPs that were associated with CAD or acute myocardial infarction (AMI), including m6A-, m1A-, m5C-, A-to-I- and m7G-related SNPs. The m6A-SNPs rs3739998 in JCAD, rs148172130 in RPL14 and rs12190287 in TCF21 and the m7G-SNP rs186643756 in PVT1 were genome-wide significant. The RNAm-SNPs were associated with gene expression (e.g., MRAS, DHX36, TCF21, JCAD and SH2B3), and the expression levels were associated with CAD. Differential m6A methylation and differential expression in FTO-overexpressing human aorta smooth muscle cells and peripheral blood mononuclear cells of CAD patients and controls were detected. The RNAm-SNPs were associated with circulating levels of proteins with specific biological functions, such as blood coagulation, and the proteins (e.g., cardiotrophin-1) were confirmed to be associated with CAD and AMI in MR analyses.

Conclusion

The present study identified RNAm-SNPs in CAD susceptibility genes, gene expression and circulating proteins as risk factors for CAD and suggested that RNA modification may play a role in the pathogenesis of CAD.
Cardiotoxicity induced by immune checkpoint inhibitor: The complete insight into mechanisms, monitoring, diagnosis, and treatment
Authors: Ganesh Sridha , Zhong Peng , Zhou Xiaoyang
Published: September 2022

Immune checkpoint inhibitors (ICIs) have been taking cancer research by storm as they provide valuable therapeutic benefits to cancer patients in terms of immunotherapy. Melanoma and non-small cell lung cancer (NSCLC) are among the most prevalent cancer varieties that were utilized in ICI trials with many other cancer types being involved too. Despite impressive clinical benefits of overall response rate (ORR), progression-free survival (PFS), etc., ICIs are also accompanied by various immune-related adverse events (irAEs). Amongst the irAEs, cardiotoxicity bags a crucial role. It is of paramount importance that ICI-induced cardiotoxicity should be studied in detail due to its high mortality rate although the prevalence rate is low. Patients with ICI cardiotoxicity can have a greatly enhanced life quality despite adverse reactions from ICI therapy if diagnosed early and treated in time. As such, this review serves to provide a complete insight into the predisposing factors, mechanism, diagnostic methods and treatment plans revolving around ICI-induced cardiotoxicity.
Confirmation of human ovulation in assisted reproduction using an adhesive axillary thermometer (femSense®)
Authors: Weiss Gregor , Strohmayer Karl , Koele Werner , Reinschissler Nina , Schenk Michael
Published: September 2022

Objective

Timing for sexual intercourse is important in achieving pregnancy in natural menstrual cycles. Different methods of detecting the fertile window have been invented, among them luteinization hormone (LH) to predict ovulation and biphasic body basal temperature (BBT) to confirm ovulation retrospectively. The gold standard to detect ovulation in gynecology practice remains transvaginal ultrasonography in combination with serum progesterone. In this study we evaluated a wearable temperature sensing patch (femSense®) using continuous body temperature measurement to confirm ovulation and determine the end of the fertile window.

Methods

96 participants received the femSense® system consisting of an adhesive axillary thermometer patch and a smartphone application, where patients were asked to document information about their previous 3 cycles. Based on the participants data, the app predicted the cycle length and the estimated day of ovulation. From these predictions, the most probable fertile window and the day for applying the patch were derived. Participants applied and activated the femSense® patch on the calculated date, from which the patch continuously recorded their body temperature throughout a period of up to 7 days to confirm ovulation. Patients documented their daily urinary LH test positivity, and a transvaginal ultrasound was performed on day cycle day 7, 10, 12 and 14/15 to investigate the growth of one dominant follicle. If a follicle reached 15 mm in diameter, an ultrasound examination was carried out every day consecutively until ovulation. On the day ovulation was detected, serum progesterone was measured to confirm the results of the ultrasound. The performance of femSense® was evaluated by comparing the day of ovulation confirmation with the results of ovulation prediction (LH test) and detection (transvaginal ultrasound).

Results

The femSense® system confirmed ovulation occurrence in 60 cases (81.1%) compared to 48 predicted cases (64.9%) with the LH test (p = 0.041). Subgroup analysis revealed a positive trend for the femSense® system of specific ovulation confirmation within the fertile window of 24 h after ovulation in 42 of 74 cases (56.8%). Cycle length, therapy method or infertility reason of the patient did not influence accuracy of the femSense® system.

Conclusions

The femSense® system poses a promising alternative to the traditional BBT method and is a valuable surrogate marker to transvaginal ultrasound for confirmation of ovulation.