bluuebunny/crossref_metadata_2025 huggingface dataset:
Inhibitory effect of t-butyl hydroperoxide on mitochondrial oxidative phosphorylation in isolated rat hepatocytes
Authors: Křiváková P , Lábajová A , Červinková Z , Drahota Z
Published: July 2021

Using high-resolution oxygraphy, we tested the changes of various parameters characterizing the mitochondrial energy provision system that were induced by peroxidative damage. In the presence of succinate as respiratory substrate, 3 mM t-butyl hydroperoxide increased respiration in the absence of ADP, which indicated partial uncoupling of oxidative phosphorylation. Low activity of coupled respiration was still maintained as indicated by the ADP-activated and oligomycin-inhibited respiration. However, during the incubation the phosphorylative capacity decreased as indicated by the continuous decrease of the mitochondrial membrane potential. Under these experimental conditions the maximum capacity of the succinate oxidase system was inhibited by 50% in comparison with values obtained in the absence of t-butyl hydroperoxide. Our data thus indicate that the oxygraphic evaluation of mitochondrial function represents a useful tool for evaluation of changes participating in peroxidative damage of cell energy metabolism.
Collective memory and means of claims in democracies: Evidence from Chile (2019-2021)
Authors: Martins Armando , Hemsley Pedro
Published: July 2021

This article argues that protests are a primary driver of political engagement in nations with a memory of institutional violence. The hypothesis is tested in the case of Chilean protests from October 2019 to March 2020 triggered by police violence Estallido Social and the Constituent Plebiscite conducted in October 2020 in response to the protests. Political violence plagues the history of Chile, in particular in the authoritarian era of the Pinochet regime. Qualitative evidence indicates a strong reaction from the civil society against this trauma. Quantitative assessment is done through Weighed LS and 2SLS estimates for 289 municipalities. The evidence suggests that where there was more political victimization in the Pinochet era, there was more engagement in the Estallido Social. However, these localities also participated less in the plebiscite and the voting on constituent members and made no more significant effort to favor any party coalition.
The Benefit of Hedgerow Access on the Health and Growth Rate of Pasture Raised Broiler Chickens
Authors: Delgadillo Eilea , Glidden Caroline , Pollak Marissa , Rysenga Holly , Jolles Anna , Beechler Brianna
Published: July 2021

Pasture rearing is an increasingly common option for small farms to produce humanely raised poultry products for an expanding market, however profit margins tend to be much lower for pastured poultry producers than for those that opt for conventional indoor rearing. Research into simple methods to optimize the growth and health of pasture-raised poultry can help small farmers maximize meat yields and decrease the morbidity and mortality of their flock, ultimately leading to higher profit margins and improved animal welfare. The objective of this study was to measure how the inclusion of mature, native foliage into pastures can impact the production performance and overall health status of two different popular breeds of commercial broiler chickens; fast-growing Cornish cross and slower growing Red rangers. During the finisher phase (30–78 days old), pastured chickens were separated by breed into either a treatment population with access to a mature bordering hedgerow, or a control population without access to a hedgerow. Weekly weight gain, daily feed intake and basic hematologic values were used to evaluate differences in the production performance and health status between each population. We found that hedge access led to a significant improvement in the rate of gain of the Cornish cross without an associated increase in feed intake, suggesting that the addition of hedges can increase meat yields in fast-growing broilers without increasing feed costs. Red rangers with hedge access demonstrated an improved ability to neutralize bacterial pathogens in whole blood and a lesser degree of hemodilution compared to control populations, suggesting improved immune function and a lower degree of heat stress in these populations. We conclude that the addition of natural environmental enrichment such as mature foliage to grass pastures can lead to improved production performance in fast-growing broilers and improvements in the health and immune function of slower growing broiler chickens.
A Feasibility Study on 3D Bioprinting of Microfat Constructs Towards Wound Healing Applications
Authors: Schmitt Trevor , Katz Nathan , Kishore Vipuil
Published: July 2021

Chronic wounds affect over 400,000 people in the United States alone, with up to 60,000 deaths each year from non-healing ulcerations. Tissue grafting (e.g., autografts, allografts, and xenografts) and synthetic skin substitutes are common treatment methods, but most solutions are limited to symptomatic treatment and do not address the underlying causes of the chronic wound. Use of fat grafts for wound healing applications has demonstrated promise but these grafts suffer from low cell viability and poor retention at the wound site resulting in suboptimal healing of chronic wounds. Herein, we report on an innovative closed-loop fat processing system (MiniTCTM) that can efficiently process lipoaspirates into microfat clusters comprising of highly viable regenerative cell population (i.e., adipose stromal cells, endothelial progenitors) preserved in their native niche. Cryopreservation of MiniTCTM isolated microfat retained cell count and viability. To improve microfat retention and engraftment at the wound site, microfat was mixed with methacrylated collagen (CMA) bioink and 3D printed to generate microfat-laden collagen constructs. Modulating the concentration of microfat in CMA constructs had no effect on print fidelity or stability of the printed constructs. Results from the Alamar blue assay showed that the cells remain viable and metabolically active in microfat-laden collagen constructs for up to 10 days in vitro. Further, quantitative assessment of cell culture medium over time using ELISA revealed a temporal expression of proinflammatory and anti-inflammatory cytokines indicative of wound healing microenvironment progression. Together, these results demonstrate that 3D bioprinting of microfat-laden collagen constructs is a promising approach to generate viable microfat grafts for potential use in treatment of non-healing chronic wounds.
MicroRNA-1205 Regulation of FRYL in Prostate Cancer
Authors: Naidoo Michelle , Levine Fayola , Gillot Tamara , Orunmuyi Akintunde T. , Olapade-Olaopa E. Oluwabunmi , Ali Thahmina , Krampis Konstantinos , Pan Chun , Dorsaint Princesca , Sboner Andrea , Ogunwobi Olorunseun O.
Published: July 2021

High mortality rates of prostate cancer (PCa) are associated with metastatic castration-resistant prostate cancer (CRPC) due to the maintenance of androgen receptor (AR) signaling despite androgen deprivation therapies (ADTs). The 8q24 chromosomal locus is a region of very high PCa susceptibility that carries genetic variants associated with high risk of PCa incidence. This region also carries frequent amplifications of the PVT1 gene, a non-protein coding gene that encodes a cluster of microRNAs including, microRNA-1205 (miR-1205), which are largely understudied. Herein, we demonstrate that miR-1205 is underexpressed in PCa cells and tissues and suppresses CRPC tumors in vivo. To characterize the molecular pathway, we identified and validated fry-like (FRYL) as a direct molecular target of miR-1205 and observed its overexpression in PCa cells and tissues. FRYL is predicted to regulate dendritic branching, which led to the investigation of FRYL in neuroendocrine PCa (NEPC). Resistance toward ADT leads to the progression of treatment related NEPC often characterized by PCa neuroendocrine differentiation (NED), however, this mechanism is poorly understood. Underexpression of miR-1205 is observed when NED is induced in vitro and inhibition of miR-1205 leads to increased expression of NED markers. However, while FRYL is overexpressed during NED, FRYL knockdown did not reduce NED, therefore revealing that miR-1205 induces NED independently of FRYL.
Emerging Importance of Chemokine Receptor CXCR4 and Its Ligand in Liver Disease
Authors: Wang Sheng , Gao Songsen , Li Yueran , Qian Xueyi , Luan Jiajie , Lv Xiongwen
Published: July 2021

Chemokine receptors are members of the G protein-coupled receptor superfamily, which together with chemokine ligands form chemokine networks to regulate various cellular functions, immune and physiological processes. These receptors are closely related to cell movement and thus play a vital role in several physiological and pathological processes that require regulation of cell migration. CXCR4, one of the most intensively studied chemokine receptors, is involved in many functions in addition to immune cells recruitment and plays a pivotal role in the pathogenesis of liver disease. Aberrant CXCR4 expression pattern is related to the migration and movement of liver specific cells in liver disease through its cross-talk with a variety of significant cell signaling pathways. An in-depth understanding of CXCR4-mediated signaling pathway and its role in liver disease is critical to identifying potential therapeutic strategies. Current therapeutic strategies for liver disease mainly focus on regulating the key functions of specific cells in the liver, in which the CXCR4 pathway plays a crucial role. Multiple challenges remain to be overcome in order to more effectively target CXCR4 pathway and identify novel combination therapies with existing strategies. This review emphasizes the role of CXCR4 and its important cell signaling pathways in the pathogenesis of liver disease and summarizes the targeted therapeutic studies conducted to date.
The Cytosolic DNA-Sensing cGAS-STING Pathway in Liver Diseases
Authors: Wang Zhilei , Chen Nian , Li Zhiyong , Xu Guang , Zhan Xiaoyan , Tang Jianyuan , Xiao Xiaohe , Bai Zhaofang
Published: July 2021

Inflammation is regulated by the host and is a protective response activated by the evolutionarily conserved immune system in response to harmful stimuli, such as dead cells or pathogens. cGAS-STING pathway is a vital natural sensor of host immunity that can defend various tissues and organs against pathogenic infection, metabolic syndrome, cellular stress and cancer metastasis. The potential impact of cGAS-STING pathway in hepatic ischemia reperfusion (I/R) injury, alcoholic/non-alcoholic steatohepatitis (ASH), hepatic B virus infection, and other liver diseases has recently attracted widespread attention. In this review, the relationship between cGAS-STING pathway and the pathophysiological mechanisms and progression of liver diseases is summarized. Additionally, we discuss various pharmacological agonists and antagonists of cGAS-STING signaling as novel therapeutics for the treatment of liver diseases. A detailed understanding of mechanisms and biology of this pathway will lay a foundation for the development and clinical application of therapies for related liver diseases.
Redefining Safety in Light of Human-Robot Interaction: A Critical Review of Current Standards and Regulations
Authors: Martinetti Alberto , Chemweno Peter K. , Nizamis Kostas , Fosch-Villaronga Eduard
Published: July 2021

Policymakers need to consider the impacts that robots and artificial intelligence (AI) technologies have on humans beyond physical safety. Traditionally, the definition of safety has been interpreted to exclusively apply to risks that have a physical impact on persons’ safety, such as, among others, mechanical or chemical risks. However, the current understanding is that the integration of AI in cyber-physical systems such as robots, thus increasing interconnectivity with several devices and cloud services, and influencing the growing human-robot interaction challenges how safety is currently conceptualised rather narrowly. Thus, to address safety comprehensively, AI demands a broader understanding of safety, extending beyond physical interaction, but covering aspects such as cybersecurity, and mental health. Moreover, the expanding use of machine learning techniques will more frequently demand evolving safety mechanisms to safeguard the substantial modifications taking place over time as robots embed more AI features. In this sense, our contribution brings forward the different dimensions of the concept of safety, including interaction (physical and social), psychosocial, cybersecurity, temporal, and societal. These dimensions aim to help policy and standard makers redefine the concept of safety in light of robots and AI’s increasing capabilities, including human-robot interactions, cybersecurity, and machine learning.
Control of Competing Thermodynamics and Kinetics in Vapor Phase Thin-Film Growth of Nitrides and Borides
Authors: Ohkubo Isao , Aizawa Takashi , Nakamura Katsumitsu , Mori Takao
Published: July 2021

Thin-film  growth is a platform technique that allows the preparation of various undeveloped materials and enables the development of novel energy generation devices. Preferred phase formation, control of crystalline orientation and quality, defect concentration, and stoichiometry in thin films are important for obtaining thin films exhibiting desired physical and chemical properties. In particular, the control of crystalline phase formation by utilizing thin-film technology favors the preparation of undeveloped materials. In this study, thin-film growth of transition metal nitride and rare-earth metal boride was performed using remote plasma–assisted molecular beam epitaxy and hybrid physical–chemical vapor deposition techniques, and was successfully achieved by tuning the competition between thermodynamics and kinetics during vapor-phase thin-film growth. Growth conditions of high crystalline quality titanium nitride thin films and high phase purity ytterbium boride thin films were not thermodynamically favorable. Appropriate control of the contribution degree of thermodynamics and kinetics during vapor-phase thin-film growth is crucial for fabricating high phase purity and high crystalline quality thin films.
Updates on Anticancer Therapy-Mediated Vascular Toxicity and New Horizons in Therapeutic Strategies
Authors: Hsu Po-Yen , Mammadova Aynura , Benkirane-Jessel Nadia , Désaubry Laurent , Nebigil Canan G.
Published: July 2021

Vascular toxicity is a frequent adverse effect of current anticancer chemotherapies and often results from endothelial dysfunction. Vascular endothelial growth factor inhibitors (VEGFi), anthracyclines, plant alkaloids, alkylating agents, antimetabolites, and radiation therapy evoke vascular toxicity. These anticancer treatments not only affect tumor vascularization in a beneficial manner, they also damage ECs in the heart. Cardiac ECs have a vital role in cardiovascular functions including hemostasis, inflammatory and coagulation responses, vasculogenesis, and angiogenesis. EC damage can be resulted from capturing angiogenic factors, inhibiting EC proliferation, survival and signal transduction, or altering vascular tone. EC dysfunction accounts for the pathogenesis of myocardial infarction, atherothrombosis, microangiopathies, and hypertension. In this review, we provide a comprehensive overview of the effects of chemotherapeutic agents on vascular toxicity leading to hypertension, microvascular rarefaction thrombosis and atherosclerosis, and affecting drug delivery. We also describe the potential therapeutic approaches such as vascular endothelial growth factor (VEGF)-B and prokineticin receptor-1 agonists to maintain endothelial function during or following treatments with chemotherapeutic agents, without affecting anti-tumor effectiveness.
IL-10 and IL-12 (P70) Levels Predict the Risk of Covid-19 Progression in Hypertensive Patients: Insights From the BRACE-CORONA Trial
Authors: Moll-Bernardes Renata , de Sousa Andrea Silvestre , Macedo Ariane V. S. , Lopes Renato D. , Vera Narendra , Maia Luciana C. R. , Feldman André , Arruda Guilherme D. A. S. , Castro Mauro J. C. , Pimentel-Coelho Pedro M. , de Albuquerque Denílson C. , de Paula Thiago Ceccatto , Furquim Thyago A. B. , Loures Vitor A. , Giusti Karla G. D. , de Oliveira Nathália M. , De Luca Fábio A. , Kotsugai Marisol D. M. , Domiciano Rafael A. M. , Santos Mayara Fraga , de Souza Olga Ferreira , Bozza Fernando A. , Luiz Ronir Raggio , Medei Emiliano
Published: July 2021

Background: Cardiovascular comorbidities such as hypertension and inflammatory response dysregulation are associated with worse COVID-19 prognoses. Different cytokines have been proposed to play vital pathophysiological roles in COVID-19 progression, but appropriate prognostic biomarkers remain lacking. We hypothesized that the combination of immunological and clinical variables at admission could predict the clinical progression of COVID-19 in hypertensive patients.

Methods: The levels of biomarkers, including C-reactive protein, lymphocytes, monocytes, and a panel of 29 cytokines, were measured in blood samples from 167 hypertensive patients included in the BRACE-CORONA trial. The primary outcome was the highest score during hospitalization on the modified WHO Ordinal Scale for Clinical Improvement. The probability of progression to severe disease was estimated using a logistic regression model that included clinical variables and biomarkers associated significantly with the primary outcome.

Results: During hospitalization, 13 (7.8%) patients showed progression to more severe forms of COVID-19, including three deaths. Obesity, diabetes, oxygen saturation, lung involvement on computed tomography examination, the C-reactive protein level, levels of 15 cytokines, and lymphopenia on admission were associated with progression to severe COVID-19. Elevated levels of interleukin-10 and interleukin-12 (p70) combined with two or three of the abovementioned clinical comorbidities were associated strongly with progression to severe COVID-19. The risk of progression to severe disease reached 97.5% in the presence of the five variables included in our model.

Conclusions: This study demonstrated that interleukin-10 and interleukin-12 (p70) levels, in combination with clinical variables, at hospital admission are key biomarkers associated with an increased risk of disease progression in hypertensive patients with COVID-19.
Evaluation of Micropiles With Different Configuration Settings for Landslide Stabilization Based on Large-Scale Experimental Testing
Authors: Liu Xueling , Yan Jinkai , Tong Bin , Liu Lei
Published: July 2021

In this study, a large-scale model test was performed to investigate the effect of the single-row and double-row micropiles on the landside stabilization. For two different testing configuration settings, the bending moment along the micropiles, failure mode, and force condition were captured and compared. It is found that the landslide thrust on piles was distributed in a triangular shape. The piles in the front row carried greater pressure than the piles in the rear row. The resistance of the sliding body behind the pile was distributed in a parabolic shape, and mainly concentrated on the middle of the pile. The piles were destroyed due to the combined shearing and bending impact applied near the slipping surface. The boundary of the failure zone was from the position of two times the pile diameter under the slipping surface to the position of two and a half times the pile diameter above the slipping surface. Under the action of the landslide, each row of piles deformed at the same time. The capability of landslide stabilization for double-row piles was better than that of a single-row pile. The sections of the pile above slide surface were mainly subjected to negative bending moments and were distributed mainly within the pile length range of one-third of the anti-sliding section above the sliding surface. The pile body of the embedded section located in the range of ten times the pile diameter below the sliding surface was subjected to a positive bending moment.
Optical Stimulation Luminescence Dating of Deltas Revealed the Early to Mid-Holocene Lake-level Fluctuations of Daihai, Inner Mongolia, Northern China
Authors: Huang Shixin , Chun Xi
Published: July 2021

Lake-level reconstruction of inland enclosed lakes especially for monsoon-sensitive areas is of great significance to reveal regional climate changes. Daihai, a typical enclosed lake at the marginal of the East Asian summer monsoon (EASM) area in north China, is sensitive to climate changes due to its unique regional characteristics. There were a series of lakeshore terraces, highstand lacustrine sediments, and braided river deltas, providing sufficient geomorphologic and stratigraphic evidence for the reconstruction of lake-level fluctuations of Daihai. Reconstructed lake-level variations during the early and mid-Holocene were constructed based on 22 quartz optical stimulated luminescence (OSL) ages from six well-preserved profiles around Daihai Basin. Our results indicated Daihai showed a relatively low level at 10.2 ka, and a gradually increasing lake level following the enhanced monsoon precipitation during the mid-Holocene. Specifically, the high lake level began to develop at 8.1 ka and reached the maximum at 5.2 ka, with ∼40 m higher than present. At this time, the lake area expanded to ∼400 km2, approximately six times as large as that of present, corresponding to the maximum monsoon precipitation and intensity of EASM during the mid-Holocene. However, our stratigraphic records showed a part of the depositional records in the north and east of the Daihai was missed after 5.2 ka, probably indicating a sudden drop of the Daihai lake level. These rapid level fluctuations were likely to be interpreted by some local scenarios and need to be further investigated in the future. Overall, the lake-level fluctuation of Daihai during the early and mid-Holocene was slightly different from that observed in the previously published regional records. Possibly, the interaction of the EASM and regional feedback from topography, and hydrology factors might have contributed to the spatial complexity and distinction.
Quantitative and Qualitative Differences in the Canonical and the Reverse Distance Effect and Their Selective Association With Arithmetic and Mathematical Competencies
Authors: Vogel Stephan E. , Faulkenberry Thomas J. , Grabner Roland H.
Published: July 2021

Understanding the relationship between symbolic numerical abilities and individual differences in mathematical competencies has become a central research endeavor in the last years. Evidence on this foundational relationship is often based on two behavioral signatures of numerical magnitude and numerical order processing: thecanonicaland thereverse distance effect.Theformerindicates faster reaction times for the comparison of numerals that are far in distance (e.g., 2 8) compared to numerals that are close in distance (e.g., 2 3). The latter indicates faster reaction times for the ordinal judgment of numerals (i.e., are numerals in ascending/descending order) that are close in distance (e.g., 2 3 4) compared to numerals that are far in distance (e.g., 2 4 6). While a substantial body of literature has reported consistent associations between thecanonical distance effectand arithmetic abilities, rather inconsistent findings have been found for thereverse distance effect. Here, we tested the hypothesis that estimates of thereverse distance effectshow qualitative differences (i.e., not all participants show areverse distance effectin the expected direction) rather than quantitative differences (i.e., all individuals show areverse distance effect, but to a different degree), and that inconsistent findings might be a consequence of this variation. We analyzed data from 397 adults who performed a computerized numerical comparison task, a computerized numerical order verification task (i.e., are three numerals presented in order or not), a paper pencil test of arithmetic fluency, as well as a standardized test to assess more complex forms of mathematical competencies. We found discriminatory evidence for the two distance effects. While estimates of thecanonical distance effectshowed quantitative differences, estimates of thereverse distance effectshowed qualitative differences.Comparisons between individuals who demonstrated an effect and individuals who demonstrated noreverse distance effectconfirmed a significant moderation on the correlation with mathematical abilities. Significantly larger effects were found in the group who showed an effect. These findings confirm that estimates of thereverse distance effectare subject to qualitative differences and that we need to better characterize the underlying mechanisms/strategies that might lead to these qualitative differences.
Circulating Ceramides- Are Origins Important for Sphingolipid Biomarkers and Treatments?
Authors: Mah Michael , Febbraio Mark , Turpin-Nolan Sarah
Published: July 2021

Biomarkers are important tools for describing the adequacy or inadequacy of biological processes (to allow for the early and accurate diagnosis) and monitoring the biological effects of intervention strategies (to identify and develop optimal dose and treatment strategies). A number of lipid biomarkers are implicated in metabolic disease and the circulating levels of these biomarkers are used in clinical settings to predict and monitor disease severity. There is convincing evidence that specific circulating ceramide species can be used as biological predictors and markers of cardiovascular disease, atherosclerosis and type 2 diabetes mellitus. Here, we review the existing literature that investigated sphingolipids as biomarkers for metabolic disease prediction. What are the advantages and disadvantages? Are circulating ceramides predominantly produced in the liver? Will hepatic sphingolipid inhibitors be able to completely prevent and treat metabolic disease? As sphingolipids are being employed as biomarkers and potential metabolic disease treatments, we explore what is currently known and what still needs to be discovered.
Hydrogen Atom and Molecule Adsorptions on FeCrAl (100) Surface: A First-Principle Study
Authors: Li Xiaojing , Ma Yu , Zhou Wenzhong , Liu Zhao
Published: July 2021

FeCrAl alloys are promising accident-tolerant fuel (ATF) cladding materials for applications in light water reactors (LWRs). Despite the excellent mechanical and antioxidation properties, this series of iron-based alloys has poor hydrogen embrittlement (HE) resistance due to the strong hydrogen uptaking ability. The hydrogen embrittlement effect can cause the degradation and premature failure of the material, and this effect can be enhanced by the high-temperature/high-pressure/high-irradiation environment in reactors. So, the potential danger should be taken seriously. In this paper, we have studied the hydrogen atom and molecule adsorptions on both Fe (100) and FeCrAl (100) surfaces to discover how the hydrogen atom and molecule (H/H2) interact with the Fe and FeCrAl (100) surface in the first place. The results show that there are strong element effects on the FeCrAl surface. The Al atom itself has no interaction with hydrogen. When the Al atom is beside the Fe atom, this Fe atom has a slightly lower interaction with hydrogen. However, the Al atom beside the Cr atom will enhance the hydrogen interaction with this Cr atom. On the other hand, when the Cr atom is beside the Fe atom, these two atoms (Fe–Cr bridge site) can reduce the interactions with H. In addition, when two Cr and two Fe atoms together make a four-fold site (FF site), the two Cr atoms can increase the interaction of the two Fe atoms with H. The element effects discovered can be a good guide for making hydrogen prevention coatings.
Habitat Preferences of Italian Freshwater Fish: A Systematic Review of Data Availability for Applications of the MesoHABSIM Model
Authors: Negro Giovanni , Fenoglio Stefano , Quaranta Emanuele , Comoglio Claudio , Garzia Isabella , Vezza Paolo
Published: July 2021

The MesoHABitat SImulation Model (MesoHABSIM) is the preferred method to calculate spatio-temporal variation in the fish habitat availability in Italian rivers. With the aim of improving the applicability of the MesoHABSIM approach in the Italian territory, we carried out a systematic review of physical habitat preferences for 31 freshwater fish species and three freshwater lampreys, representing 75% of the total indigenous freshwater fish community of Italy. Information related to suitable ranges of depth, flow velocity, biotic/abiotic substrates, covers/shelters was collected and summarized for two critical life stages (adult and juvenile) and two bioperiods (rearing/growth and spawning). Overall, 250 publications were reviewed, classified as 206 peer-reviewed papers, 20 books, 7 PhD thesis, and 17 grey literature sources. Our analysis revealed substantial deficits of information about habitat requirements for more than 30% of Italian freshwater fish species. This information is particularly scarce for the most threatened endemic species, especially for their most critical bioperiod (i.e., spawning). With the aim of preserving freshwater fish biodiversity as required in the EU Biodiversity Strategy for 2030 (European Commission, 2020), accurate information on physical habitat requirements for spawning is crucial. As an example application of MesoHABSIM, the collected habitat preference information was used to define and apply mesohabitat suitability criteria for one fish species (Telestes muticellus) in a regulated river reach of Argentina Creek (Province of Imperia, Italy). This analysis demonstrates the potential for applying information from the current review to other fish species.
Environmental Change Threatens Freshwater Insect Communities in Northwest Africa: A Meta-Analysis
Authors: Kaczmarek Nils , Schäfer Ralf B. , Berger Elisabeth
Published: July 2021

A climatic shift from temperate to arid conditions is predicted for Northwest Africa. Water temperature, salinity, and river intermittency are likely to increase, which may impact freshwater communities, ecosystem functioning, and related ecosystem services. Quantitative data and information on the impact of climate change on insect communities (e.g., richness, taxonomic and trait composition) are still scarce for Northwest Africa. In this study, we extracted information on freshwater insect occurrence and environmental variables in Northwest Africa from the results of a literature search to study potential consequences of changing climatic conditions for these communities. Our data set covered 96 families in 165 sites in Morocco and Algeria. We quantified the impact of several explanatoryvariables (climate, altitude, water temperature, conductivity, intermittency, flow, aridity, dams, and land cover) on richness, taxonomic and functional trait composition using negative binomial regression models and constrained ordination. Family richness in arid sites was on average 37 % lower than in temperate sites in association with flow, river regulation, cropland extent, conductivity, altitude, and water temperature. With 36 % of the studied temperate sites predicted to turn arid by the end of the century, a loss of insect families can be predicted for Northwest Africa, mainly affecting species adapted to temperate environments. Resistance and resilience traits such as small body size, aerial dispersal, and air breathing promote survival in arid climates. Future research should report insect occurrences on species level to allow for better predictions on climate change effects.
Odour Learning Bees Have Longer Foraging Careers Than Non-learners in a Natural Environment
Authors: Evans Lisa J. , Smith Karen E. , Raine Nigel E.
Published: July 2021

Individual animals allowed the opportunity to learn generally outperform those prevented from learning, yet, within a species the capacity for learning varies markedly. The evolutionary processes that maintain this variation in learning ability are not yet well understood. Several studies demonstrate links between fitness traits and visual learning, but the selection pressures operating on cognitive traits are likely influenced by multiple sensory modalities. In addition to vision, most animals will use a combination of hearing, olfaction (smell), gustation (taste), and touch to gain information about their environment. Some animals demonstrate individual preference for, or enhanced learning performance using certain senses in relation to particular aspects of their behaviour (e.g., foraging), whereas conspecific individuals may show different preferences. By assessing fitness traits in relation to different sensory modalities we will strengthen our understanding of factors driving observed variation in learning ability. We assessed the relationship between the olfactory learning ability of bumble bees (Bombus terrestris) and their foraging performance in their natural environment. We found that bees which failed to learn this odour-reward association had shorter foraging careers; foraging for fewer days and thus provisioning their colonies with fewer resources. This was not due to a reduced propensity to forage, but may have been due to a reduced ability to return to their colony. When comparing among only individuals that did learn, we found that the rate at which floral resources were collected was similar, regardless of how they performed in the olfactory learning task. Our results demonstrate that an ability to learn olfactory cues can have a positive impact of the foraging performance of B. terrestris in a natural environment, but echo findings of earlier studies on visual learning, which suggest that enhanced learning is not necessarily beneficial for bee foragers provisioning their colony.
Culture-Based and Culture-Independent Assessments of Endophytic Fungal Diversity in Aquatic Plants in Southwest China
Authors: Zheng Hua , Qiao Min , Xu Jianping , Yu Zefen
Published: July 2021

Aquatic ecosystems contain tremendous plant and microbial diversity. However, little is known about endophyte diversity in aquatic plants. In this study, we investigated the diversity of endophytic fungi in aquatic plants in southwest China using both culture-based and culture-independent high-throughput sequencing methods. A total of 1,689 fungal isolates belonging to three phyla and 154 genera were obtained from 15,373 plant tissue segments of 30 aquatic plant species. The most abundant endophytic fungi were those in ascomycete genera Aspergillus, Ceratophoma, Fusarium, Penicillium, Phoma and Plectosporium. No difference in fungal isolation rates was observed among tissues from roots, stems, and leaves. Twenty tissue samples from three most common plant species were further subjected to culture-independent meta-barcode sequencing. The sequence-based analyses revealed a total of 1,074 OTUs belonging to six fungal phyla and 194 genera. Among the three plants, Batrachium bungei harbored the highest number of OTUs. Besides, a total of 66 genera were detected by two methods. Both the culture-dependent and independent methods revealed that aquatic plants in southwest China have abundant endophytic fungal diversity. This study significantly expands our knowledge of the fungal community of aquatic plants.
Long Non-coding RNA: An Emerging Contributor and Potential Therapeutic Target in Renal Fibrosis
Authors: Xia Weiping , He Yao , Gan Yu , Zhang Bo , Dai Guoyu , Ru Feng , Jiang Zexiang , Chen Zhi , Chen Xiang
Published: July 2021

Renal fibrosis (RF) is a pathological process that culminates in terminal renal failure in chronic kidney disease (CKD). Fibrosis contributes to progressive and irreversible decline in renal function. However, the molecular mechanisms involved in RF are complex and remain poorly understood. Long non-coding RNAs (lncRNAs) are a major type of non-coding RNAs, which significantly affect various disease processes, cellular homeostasis, and development through multiple mechanisms. Recent investigations have implicated aberrantly expressed lncRNA in RF development and progression, suggesting that lncRNAs play a crucial role in determining the clinical manifestation of RF. In this review, we comprehensively evaluated the recently published articles on lncRNAs in RF, discussed the potential application of lncRNAs as diagnostic and/or prognostic biomarkers, proposed therapeutic targets for treating RF-associated diseases and subsequent CKD transition, and highlight future research directions in the context of the role of lncRNAs in the development and treatment of RF.
Comparative Analysis of the Chloroplast Genome for Four Pennisetum Species: Molecular Structure and Phylogenetic Relationships
Authors: Xu Jin , Liu Chen , Song Yun , Li Mingfu
Published: July 2021

The genus Pennisetum (Poaceae) is both a forage crop and staple food crop in the tropics. In this study, we obtained chloroplast genome sequences of four species of Pennisetum (P. alopecuroides, P. clandestinum, P. glaucum, and P. polystachion) using Illumina sequencing. These chloroplast genomes have circular structures of 136,346–138,119 bp, including a large single-copy region (LSC, 79,380–81,186 bp), a small single-copy region (SSC, 12,212–12,409 bp), and a pair of inverted repeat regions (IRs, 22,284–22,372 bp). The overall GC content of these chloroplast genomes was 38.6–38.7%. The complete chloroplast genomes contained 110 different genes, including 76 protein-coding genes, 30 transfer RNA (tRNA) genes, and four ribosomal RNA (rRNA) genes. Comparative analysis of nucleotide variability identified nine intergenic spacer regions (psbA-matK, matK-rps16, trnN-trnT, trnY-trnD-psbM, petN-trnC, rbcL-psaI, petA-psbJ, psbE-petL, and rpl32-trnL), which may be used as potential DNA barcodes in future species identification and evolutionary analysis of Pennisetum. The phylogenetic analysis revealed a close relationship between P. polystachion and P. glaucum, followed by P. clandestinum and P. alopecuroides. The completed genomes of this study will help facilitate future research on the phylogenetic relationships and evolution of Pennisetum species.
Novel Insights on MRGPRX2-Mediated Hypersensitivity to Neuromuscular Blocking Agents And Fluoroquinolones
Authors: Elst Jessy , Maurer Marcus , Sabato Vito , Faber Margaretha A. , Bridts Chris H. , Mertens Christel , Van Houdt Michel , Van Gasse Athina L. , van der Poorten Marie-Line M. , De Puysseleyr Leander P. , Hagendorens Margo M. , Van Tendeloo Viggo F. , Lion Eva , Campillo-Davo Diana , Ebo Didier G.
Published: July 2021

Neuromuscular blocking agents (NMBAs) like atracurium and rocuronium as well as fluoroquinolones (FQs) cause mast cell-mediated anaphylaxis by activating Mas-related G protein-coupled receptor X2 (MRGPRX2), but many questions remain unanswered. Here, we address three of them, namely whether primary human mast cells show similar activation by these drugs as murine mast cells and mast cell lines, how sugammadex protects from atracurium-induced MRGPRX2-mediated mast cell activation, and why some but not all patients treated with rocuronium develop anaphylaxis. We used peripheral blood-derived cultured mast cells from healthy donors and patients, assessed mast cell activation and degranulation by quantifying intracellular calcium and CD63 expression, respectively, and made use of MRGPRX2-silencing, via electroporation with Dicer-substrate small interfering RNAs, and single cell flow cytometric analyses. Atracurium, ciprofloxacin, and levofloxacin activated and degranulated primary human mast cells, but only MRGPRX2-positive and not MRGPRX2-negative or -silenced mast cells. Sugammadex attenuated the atracurium-induced and MRGPRX2-mediated activation and degranulation of human mast cells by reducing free atracurium levels. The mast cells of patients with IgE-independent anaphylaxis to rocuronium were similar, in their MRGPRX2 expression and function, to those of patients with IgE-mediated anaphylaxis. These findings further improve our understanding of the role and relevance of MRGPRX2-driven mast cell activation in anaphylactic reactions to NMBAs and FQs and may help to improve their prediction, prevention, and treatment.
Congenital Deficiency of Conventional Dendritic Cells Promotes the Development of Atopic Dermatitis-Like Inflammation
Authors: Nishikawa Yotaro , Fukaya Tomohiro , Fukui Takehito , Uto Tomofumi , Takagi Hideaki , Nasu Junta , Miyanaga Noriaki , Riethmacher Dieter , Choijookhuu Narantsog , Hishikawa Yoshitaka , Amano Masahiro , Sato Katsuaki
Published: July 2021

Atopic dermatitis (AD) is a common pruritic inflammatory skin disease characterized by impaired epidermal barrier function and dysregulation of Thelper-2 (TH2)-biased immune responses. While the lineage of conventional dendritic cells (cDCs) are implicated to play decisive roles in T-cell immune responses, their requirement for the development of AD remains elusive. Here, we describe the impact of the constitutive loss of cDCs on the progression of AD-like inflammation by using binary transgenic (Tg) mice that constitutively lacked CD11chi cDCs. Unexpectedly, the congenital deficiency of cDCs not only exacerbates the pathogenesis of AD-like inflammation but also elicits immune abnormalities with the increased composition and function of granulocytes and group 2 innate lymphoid cells (ILC2) as well as B cells possibly mediated through the breakdown of the Fms-related tyrosine kinase 3 ligand (Flt3L)-mediated homeostatic feedback loop. Furthermore, the constitutive loss of cDCs accelerates skin colonization of Staphylococcus aureus (S. aureus), that associated with disease flare. Thus, cDCs maintains immune homeostasis to prevent the occurrence of immune abnormalities to maintain the functional skin barrier for mitigating AD flare.
Polyfunctional KLRG-1+CD57+ Senescent CD4+ T Cells Infiltrate Tumors and Are Expanded in Peripheral Blood From Breast Cancer Patients
Authors: Ramello Maria C. , Núñez Nicolás G. , Tosello Boari Jimena , Bossio Sabrina N. , Canale Fernando P. , Abrate Carolina , Ponce Nicolas , Del Castillo Andrés , Ledesma Marta , Viel Sophie , Richer Wilfrid , Sedlik Christine , Tiraboschi Carolina , Muñoz Marcos , Compagno Daniel , Gruppi Adriana , Acosta Rodríguez Eva V. , Piaggio Eliane , Montes Carolina L.
Published: July 2021

Senescent T cells have been described during aging, chronic infections, and cancer; however, a comprehensive study of the phenotype, function, and transcriptional program of this T cell population in breast cancer (BC) patients is missing. Compared to healthy donors (HDs), BC patients exhibit an accumulation of KLRG-1+CD57+CD4+and CD8+T cells in peripheral blood. These T cells infiltrate tumors and tumor-draining lymph nodes. KLRG-1+CD57+CD4+and CD8+T cells from BC patients and HDs exhibit features of senescence, and despite their inhibitory receptor expression, they produce more effector cytokines and exhibit higher expression of Perforin, Granzyme B, and CD107a than non-senescent subsets. When compared to blood counterparts, tumor-infiltrating senescent CD4+T cells show similar surface phenotype but reduced cytokine production. Transcriptional profiling of senescent CD4+T cells from the peripheral blood of BC patients reveals enrichment in genes associated with NK or CD8+-mediated cytotoxicity, TCR-mediated stimulation, and cell exhaustion compared to non-senescent T cells. Comparison of the transcriptional profile of senescent CD4+T cells from peripheral blood of BC patients with those of HDs highlighted marked similarities but also relevant differences. Senescent CD4+T cells from BC patients show enrichment in T-cell signaling, processes involved in DNA replication, p53 pathways, oncogene-induced senescence, among others compared to their counterparts in HDs. High gene expression of CD4, KLRG-1, and B3GAT1 (CD57), which correlates with increased overall survival for BC patients, underscores the usefulness of the evaluation of the frequency of senescent CD4+T cells as a biomarker in the follow-up of patients.
Gene-Edited Interleukin CAR-T Cells Therapy in the Treatment of Malignancies: Present and Future
Authors: Zhang Zhengchao , Miao Lele , Ren Zhijian , Tang Futian , Li Yumin
Published: July 2021

In recent years, chimeric antigen receptor T cells (CAR-T cells) have been faced with the problems of weak proliferation and poor persistence in the treatment of some malignancies. Researchers have been trying to perfect the function of CAR-T by genetically modifying its structure. In addition to the participation of T cell receptor (TCR) and costimulatory signals, immune cytokines also exert a decisive role in the activation and proliferation of T cells. Therefore, genetic engineering strategies were used to generate cytokines to enhance tumor killing function of CAR-T cells. When CAR-T cells are in contact with target tumor tissue, the proliferation ability and persistence of T cells can be improved by structurally or inductively releasing immunoregulatory molecules to the tumor region. There are a large number of CAR-T cells studies on gene-edited cytokines, and the most common cytokines involved are interleukins (IL-7, IL-12, IL-15, IL-18, IL-21, IL-23). Methods for the construction of gene-edited interleukin CAR-T cells include co-expression of single interleukin, two interleukin, interleukin combined with other cytokines, interleukin receptors, interleukin subunits, and fusion inverted cytokine receptors (ICR). Preclinical and clinical trials have yielded positive results, and many more are under way. By reading a large number of literatures, we summarized the functional characteristics of some members of the interleukin family related to tumor immunotherapy, and described the research status of gene-edited interleukin CAR-T cells in the treatment of malignant tumors. The objective is to explore the optimized strategy of gene edited interleukin-CAR-T cell function.