bluuebunny/crossref_metadata_2025 huggingface dataset:
Differentiated Thyroid Cancer with Biochemical Incomplete Response: Clinico-Pathological Characteristics and Long Term Disease Outcomes
Authors: Steinschneider Miriam , Pitaro Jacob , Koren Shlomit , Mizrakli Yuval , Benbassat Carlos , Muallem Kalmovich Limor
Published: November 2021

Although most patients with differentiated thyroid cancer (DTC) and biochemical incomplete response (BIR) follow a good clinical outcome, progression to structural disease may occur in 8–17% of patients. We aimed to identify factors that could predict the long-term outcomes of BIR patients. To this end, we conducted a retrospective review study of 1049 charts from our Differential Thyroid Cancer registry of patients who were initially treated with total thyroidectomy between 1962 and 2019. BIR was defined as suppressed thyroglobulin (Tg) > 1 ng/mL, stimulated Tg > 10 ng/mL or rising anti-Tg antibodies, who did not have structural evidence of disease, and who were assessed 12–24 months after initial treatment. We found 83 patients (7.9%) matching the definition of BIR. During a mean follow-up of 12 ± 6.6 years, 49 (59%) patients remained in a state of BIR or reverted to no evidence of disease, while 34 (41%) progressed to structural disease. At the last follow-up, three cases (3.6%) were recorded as disease-related death. The American Thyroid Association (ATA) Initial Risk Stratification system and/or AJCC/TNM (8th ed.) staging system at diagnosis predicted the shift from BIR to structural disease, irrespective of their postoperative Tg levels. We conclude that albeit 41% of BIR patients may shift to structural disease, and most have a rather indolent disease. Specific new individual data enable the Response to Therapy reclassification to become a dynamic system to allow for the better management of BIR patients in the long term.
Can Conization Specimens Predict Sentinel Lymph Node Status in Early-Stage Cervical Cancer? A SENTICOL Group Study
Authors: Balaya Vincent , Guani Benedetta , Mereaux Julie , Magaud Laurent , Pache Basile , Bonsang-Kitzis Hélène , Ngô Charlotte , Desseauve David , Mathevet Patrice , Lécuru Fabrice ,   on behalf of the SENTICOL Group
Published: November 2021

Background: The prognosis of patients with cervical cancer is significantly worsened in case of lymph node involvement. The goal of this study was to determine whether pathologic features in conization specimens can predict the sentinel lymph node (SLN) status in early-stage cervical cancer. Methods: An ancillary analysis of two prospective multicentric database on SLN biopsy for cervical cancer (SENTICOL I and II) was carried out. Patients with IA to IB2 2018 FIGO stage, who underwent preoperative conization before SLN biopsy were included. Results: Between January 2005 and July 2012, 161 patients from 25 French centers fulfilled the inclusion criteria. Macrometastases, micrometastases and Isolated tumor cells (ITCs) were found in 4 (2.5%), 6 (3.7%) and 5 (3.1%) patients respectively. Compared to negative SLN patients, patients with micrometastatic and macrometastatic SLN were more likely to have lymphovascular space invasion (LVSI) (60% vs. 29.5%, p = 0.04) and deep stromal invasion (DSI) ≥ 10 mm (50% vs. 17.8%, p = 0.04). Among the 93 patients with DSI < 10 mm and absence of LVSI on conization specimens, three patients (3.2%) had ITCs and only one (1.1%) had micrometastases. Conclusions: Patients with DSI < 10 mm and no LVSI in conization specimens had lower risk of micro- and macrometastatic SLN. In this subpopulation, full node dissection may be questionable in case of SLN unilateral detection.
Peanut-Shaped Gold Nanoparticles with Shells of Ceragenin CSA-131 Display the Ability to Inhibit Ovarian Cancer Growth In Vitro and in a Tumor Xenograft Model
Authors: Piktel Ewelina , Oscilowska Ilona , Suprewicz Łukasz , Depciuch Joanna , Marcińczyk Natalia , Chabielska Ewa , Wolak Przemysław , Głuszek Katarzyna , Klimek Justyna , Zieliński Piotr M. , Marzec Michał T. , Savage Paul B. , Parlińska-Wojtan Magdalena , Bucki Robert
Published: November 2021

Gold nanoparticles-assisted delivery of antineoplastics into cancerous cells is presented as an effective approach for overcoming the limitations of systemic chemotherapy. Although ceragenins show great potential as anti-cancer agents, in some tumors, effective inhibition of cancer cells proliferation requires application of ceragenins at doses within their hemolytic range. For the purpose of toxicity/efficiency ratio control, peanut-shaped gold nanoparticles (AuP NPs) were functionalized with a shell of ceragenin CSA-131 and the cytotoxicity of AuP@CSA-131 against ovarian cancer SKOV-3 cells and were then analyzed. In vivo efficiency of intravenously and intratumorally administered CSA-131 and AuP@CSA-131 was examined using a xenograft ovarian cancer model. Serum parameters were estimated using ELISA methods. Comparative analysis revealed that AuP@CSA-131 exerted stronger anti-cancer effects than free ceragenin, which was determined by enhanced ability to induce caspase-dependent apoptosis and autophagy processes via reactive oxygen species (ROS)-mediated pathways. In an animal study, AuP@CSA-131 was characterized by delayed clearance and prolonged blood circulation when compared with free ceragenin, as well as enhanced anti-tumor efficiency, particularly when applied intratumorally. Administration of CSA-131 and AuP@CSA-131 prevented the inflammatory response associated with cancer development. These results present the possibility of employing non-spherical gold nanoparticles as an effective nanoplatform for the delivery of antineoplastics for the treatment of ovarian malignancy.
Molecular Minimal Residual Disease Detection in Acute Myeloid Leukemia
Authors: Vonk Christian M. , Al Hinai Adil S. A. , Hanekamp Diana , Valk Peter J. M.
Published: November 2021

Initial induction chemotherapy to eradicate the bulk of acute myeloid leukemia (AML) cells results in complete remission (CR) in the majority of patients. However, leukemic cells persisting in the bone marrow below the morphologic threshold remain unaffected and have the potential to proliferate and re-emerge as AML relapse. Detection of minimal/measurable residual disease (MRD) is a promising prognostic marker for AML relapse as it can assess an individual patients’ risk profile and evaluate their response to treatment. With the emergence of molecular techniques, such as next generation sequencing (NGS), a more sensitive assessment of molecular MRD markers is available. In recent years, the detection of MRD by molecular assays and its association with AML relapse and survival has been explored and verified in multiple studies. Although most studies show that the presence of MRD leads to a worse clinical outcome, molecular-based methods face several challenges including limited sensitivity/specificity, and a difficult distinction between mutations that are representative of AML rather than clonal hematopoiesis. This review describes the studies that have been performed using molecular-based assays for MRD detection in the context of other MRD detection approaches in AML, and discusses limitations, challenges and opportunities.
Salvage Chemotherapy in Patients with Previously Treated Thymic Carcinoma
Authors: Kaira Kyoichi , Imai Hisao , Yamaguchi Ou , Mouri Atsuto , Kagamu Hiroshi
Published: November 2021

Thymic carcinoma is a rare neoplasm, and it is difficult to achieve complete remission with systemic chemotherapy. In advanced or recurrent thymic carcinoma, platinum-based chemotherapy is chosen as the first-line setting; however, it remains unclear which regimen is better to improve its outcome. It remains unknown whether salvage chemotherapy should be administered to patients with platinum-based chemotherapy-refractory thymic carcinoma. Currently, several clinical studies have investigated the efficacy of second-line settings for advanced thymic carcinoma. As cytotoxic agents, S-1, amrubicin, pemetrexed, docetaxel, paclitaxel, and gemcitabine have been reported as prospective phase II studies or retrospective studies. The overall response rates (ORRs) of S-1, amrubicin, and pemetrexed were 25–50%, 11–44.4%, and 9–10%, respectively. Molecular targeting drugs, such as sunitinib, everolimus, and lenvatinib, also provide clinical effectiveness with tolerability after the failure of platinum-based regimens. Based on the results of the prospective phase II study, the ORR, median progression-free survival, and median overall survival were 16.6% and 5.6 months, respectively, in everolimus, 26% and 7.2 months, respectively, in sunitinib, and 38% and 9.3 months, respectively, in lenvatinib. Although it is difficult to compare each study, lenvatinib appears to be better in increasing efficacy as a second-line setting. However, each study had a small sample size, which may have biased the results of their studies. Further investigation is warranted to elucidate the therapeutic significance of salvage chemotherapy in advanced thymic carcinoma in a large-scale study.
Enhancement of NK Cell Antitumor Effector Functions Using a Bispecific Single Domain Antibody Targeting CD16 and the Epidermal Growth Factor Receptor
Authors: Toffoli Elisa C. , Sheikhi Abdolkarim , Lameris Roeland , King Lisa A. , van Vliet Amanda , Walcheck Bruce , Verheul Henk M. W. , Spanholtz Jan , Tuynman Jurriaan , de Gruijl Tanja D. , van der Vliet Hans J.
Published: November 2021

The ability to kill tumor cells while maintaining an acceptable safety profile makes Natural Killer (NK) cells promising assets for cancer therapy. Strategies to enhance the preferential accumulation and activation of NK cells in the tumor microenvironment can be expected to increase the efficacy of NK cell-based therapies. In this study, we show binding of a novel bispecific single domain antibody (VHH) to both CD16 (FcRγIII) on NK cells and the epidermal growth factor receptor (EGFR) on tumor cells of epithelial origin. The bispecific VHH triggered CD16- and EGFR-dependent activation of NK cells and subsequent lysis of tumor cells, regardless of the KRAS mutational status of the tumor. Enhancement of NK cell activation by the bispecific VHH was also observed when NK cells of colorectal cancer (CRC) patients were co-cultured with EGFR expressing tumor cells. Finally, higher levels of cytotoxicity were found against patient-derived metastatic CRC cells in the presence of the bispecific VHH and autologous peripheral blood mononuclear cells or allogeneic CD16 expressing NK cells. The anticancer activity of CD16-EGFR bispecific VHHs reported here merits further exploration to assess its potential therapeutic activity either alone or in combination with adoptive NK cell-based therapeutic approaches.
New Look of EBV LMP1 Signaling Landscape
Authors: Wang Ling , Ning Shunbin
Published: November 2021

The Epstein–Barr Virus (EBV) principal oncoprotein Latent Membrane Protein 1 (LMP1) is a member of the Tumor Necrosis Factor Receptor (TNFR) superfamily with constitutive activity. LMP1 shares many features with Pathogen Recognition Receptors (PRRs), including the use of TRAFs, adaptors, and kinase cascades, for signal transduction leading to the activation of NFκB, AP1, and Akt, as well as a subset of IRFs and likely the master antioxidative transcription factor NRF2, which we have gradually added to the list. In recent years, we have discovered the Linear UBiquitin Assembly Complex (LUBAC), the adaptor protein LIMD1, and the ubiquitin sensor and signaling hub p62, as novel components of LMP1 signalosome. Functionally, LMP1 is a pleiotropic factor that reprograms, balances, and perturbs a large spectrum of cellular mechanisms, including the ubiquitin machinery, metabolism, epigenetics, DNA damage response, extracellular vehicles, immune defenses, and telomere elongation, to promote oncogenic transformation, cell proliferation and survival, anchorage-independent cell growth, angiogenesis, and metastasis and invasion, as well as the development of the tumor microenvironment. We have recently shown that LMP1 induces p62-mediated selective autophagy in EBV latency, at least by contributing to the induction of p62 expression, and Reactive Oxygen Species (ROS) production. We have also been collecting evidence supporting the hypothesis that LMP1 activates the Keap1-NRF2 pathway, which serves as the key antioxidative defense mechanism. Last but not least, our preliminary data shows that LMP1 is associated with the deregulation of cGAS-STING DNA sensing pathway in EBV latency. A comprehensive understanding of the LMP1 signaling landscape is essential for identifying potential targets for the development of novel strategies towards targeted therapeutic applications.
Targeting of HSP70/HSF1 Axis Abrogates In Vitro Ibrutinib-Resistance in Chronic Lymphocytic Leukemia
Authors: Frezzato Federica , Visentin Andrea , Severin Filippo , Pizzo Serena , Ruggeri Edoardo , Mouawad Nayla , Martinello Leonardo , Pagnin Elisa , Trimarco Valentina , Tonini Alessia , Carraro Samuela , Pravato Stefano , Imbergamo Silvia , Manni Sabrina , Piazza Francesco , Brunati Anna Maria , Facco Monica , Trentin Livio
Published: November 2021

The Btk inhibitor ibrutinib has significantly changed the management of chronic lymphocytic leukemia (CLL) patients. Despite its clinical efficacy, relapses occur, and outcomes after ibrutinib failure are poor. Although BTK and PLCγ2 mutations have been found to be associated with ibrutinib resistance in a fair percentage of CLL patients, no information on resistance mechanisms is available in patients lacking these mutations. The heat shock protein of 70 kDa (HSP70) and its transcription factor heat shock factor 1 (HSF1) play a role in mediating the survival and progression of CLL, as well as taking part in drug resistance in various cancers. We demonstrated that resveratrol and related phenols were able to induce apoptosis in vitro in leukemic cells from CLL untreated patients by acting on the HSP70/HSF1 axis. The same was achieved in cells recovered from 13 CLL patients failing in vivo ibrutinib treatment. HSP70 and HSF1 levels decreased following in vitro treatment, correlating to apoptosis induction. We suggest an involvement of HSP70/HSF1 axis in controlling resistance to ibrutinib in CLL cells, since their inhibition is effective in inducing in vitro apoptosis in cells from ibrutinib refractory patients. The targeting of HSP70/HSF1 axis could represent a novel rational therapeutic strategy for CLL, also for relapsing patients.
Contemporary Grading of Prostate Cancer: The Impact of Grading Criteria and the Significance of the Amount of Intraductal Carcinoma
Authors: Tzelepi Vasiliki , Grypari Ioanna Maria , Logotheti Souzana , Kontogiannis Stavros , Kallidonis Panagiotis , Melachrinou Maria , Zolota Vasiliki
Published: November 2021

(1) Background: Prognostic grade group (PGG) is an important prognostic parameter in prostate cancer that guides therapeutic decisions. The cribriform pattern and intraductal carcinoma (IDC) are two histological patterns, that have additional prognostic significance. However, discrepancies exist regarding the handling of IDC according to the guidelines published by two international genitourinary pathology societies. Furthermore, whether, in addition to its presence, the amount of IDC is also of importance has not been studied before. Lastly, the handling of tertiary patterns has also been a matter of debate in the literature. (2) Methods: A total of 129 prostatectomy cases were retrieved and a detailed histopathologic analysis was performed. (3) Results: Two cases (1.6%) upgraded their PGG, when IDC was incorporated in the grading system. The presence and the amount of IDC, as well as the presence of cribriform carcinoma were associated with adverse pathologic characteristics. Interestingly, in six cases (4.7%) there was a difference in PGG when using the different guidelines regarding the handling of tertiary patterns. In total, 6.2% of the cases would be assigned a different grade depending on the guidelines followed. (4) Conclusions: These findings highlight a potential area of confusion among pathologists and clinicians and underscore the need for a consensus grading system.
Current Therapeutic Strategies in Patients with Oropharyngeal Squamous Cell Carcinoma: Impact of the Tumor HPV Status
Authors: Bozec Alexandre , Culié Dorian , Poissonnet Gilles , Demard François , Dassonville Olivier
Published: November 2021

Since there is no published randomized study comparing surgical and non-surgical therapeutic strategies in patients with oropharyngeal squamous cell carcinoma (OPSCC), the therapeutic management of these patients remains highly controversial. While human papillomavirus (HPV)-positive and HPV-negative OPSCC are now recognized as two distinct diseases with different epidemiological, biological, and clinical characteristics, the impact of HPV status on the management of OPSCC patients is still unclear. In this review, we analyze the current therapeutic options in patients with OPSCC, highlighting the most recent advances in surgical and non-surgical therapies, and we discuss the impact of HPV status on the therapeutic strategy.
CD95L Inhibition Impacts Gemcitabine-Mediated Effects and Non-Apoptotic Signaling of TNF-α and TRAIL in Pancreatic Tumor Cells
Authors: Rashid Khalid , Röder Christian , Goumas Freya , Egberts Jan-Hendrik , Kalthoff Holger
Published: November 2021

Despite the potential apoptotic functions, the CD95/CD95L system can stimulate survival as well as pro-inflammatory signaling, particularly through the activation of NFκB. This holds true for the TNF/TNFR and the TRAIL/TRAILR systems. Thus, signaling pathways of these three death ligands converge, yet the specific impact of the CD95/CD95L system in this crosstalk has not been well studied. In this study, we show that gemcitabine stimulates the expression of pro-inflammatory cytokines, such as IL6 and IL8, under the influence of the CD95/CD95L system and the pharmacological inhibitor, sCD95Fc, substantially reduced the expression in two PDAC cell lines, PancTuI-luc and A818-4. The stem cell phenotype was reduced when induced upon gemcitabine as well by sCD95Fc. Moreover, TNF-α as well as TRAIL up-regulate the expression of CD95 and CD95L in both cell lines. Conversely, we detected a significant inhibitory effect of sCD95Fc on the expression of both IL8 and IL6 induced upon TNF-α and TRAIL stimulation. In vivo, CD95L inhibition reduced xeno-transplanted recurrent PDAC growth. Thus, our findings indicate that inhibition of CD95 signaling altered the chemotherapeutic effects of gemcitabine, not only by suppressing the pro-inflammatory responses that arose from the CD95L-positive tumor cells but also from the TNF-α and TRAIL signaling in a bi-lateral crosstalk manner.
Genomic Instability of Circulating Tumor DNA as a Prognostic Marker for Pancreatic Cancer Survival: A Prospective Cohort Study
Authors: Woo Sang Myung , Kim Min Kyeong , Park Boram , Cho Eun-Hae , Lee Tae-Rim , Ki Chang-Seok , Yoon Kyong-Ah , Kim Yun-Hee , Choi Wonyoung , Kim Do Yei , Hwang Jin-Hyeok , Cho Jae Hee , Han Sung-Sik , Lee Woo Jin , Park Sang-Jae , Kong Sun-Young
Published: November 2021

Genomic instability of circulating tumor DNA (ctDNA) as a prognostic biomarker has not been evaluated in pancreatic cancer. We investigated the role of the genomic instability index of ctDNA in pancreatic ductal adenocarcinoma (PDAC). We prospectively enrolled 315 patients newly diagnosed with resectable (n = 110), locally advanced (n = 78), and metastatic (n = 127) PDAC from March 2015 through January 2020. Low-depth whole-genome cell-free DNA sequencing identified genome-wide copy number alterations using instability score (I-score) to reflect genome-wide instability. Plasma cell-free and matched tumor tissue DNA from 15 patients with resectable pancreatic cancer was sequenced to assess the concordance of chromosomal copy number alteration profiles. Associations of I-score with clinical factors or survival were assessed. Seventy-six patients had high genomic instability with I-score > 7.3 in pre-treatment ctDNA; proportions of high I-score were 5.5%, 5.1%, and 52% in resectable, locally advanced, and metastatic stages, respectively. Correlation coefficients between Z-scores of plasma and tissue DNA at segment resolution were high (r2 = 0.82). Univariable analysis showed the association of I-score with progression-free survival in each stage. Multivariable analyses demonstrated that clinical stage-adjusted I-scores were significant factors for progression-free and overall survival. In these patients, ctDNA genomic I-scores provided prognostic information relevant to progression-free survival in each clinical stage.
SMARCA4 Depletion Induces Cisplatin Resistance by Activating YAP1-Mediated Epithelial-to-Mesenchymal Transition in Triple-Negative Breast Cancer
Authors: Kim Jihyun , Jang Gyubeom , Sim Sung Hoon , Park In Hae , Kim Kyungtae , Park Charny
Published: November 2021

The role of SMARCA4, an ATPase subunit of the SWI/SNF chromatin remodeling complex, in genomic organization is well studied in various cancer types. However, its oncogenic role and therapeutic implications are relatively unknown in triple-negative breast cancer (TNBC). We investigated the clinical implication and downstream regulation induced by SMARCA4 inactivation using large-scale genome and transcriptome profiles. Additionally, SMARCA4 was knocked out in MDA-MB-468 and MDA-MB-231 using CRISPR/Cas9 to identify gene regulation and a targetable pathway. First, we observed an increase in SMARCA4 mutations in cisplatin resistance and metastasis in TNBC patients. Its inactivation was associated with the mesenchymal-like (MSL) subtype. Gene expression analysis showed that the epithelial-to-mesenchymal transition (EMT) pathway was activated in SMARCA4-deficient patients. Next, the Hippo pathway was activated in the SMARCA4 inactivation group, as evidenced by the higher CTNNB1, TGF-β, and YAP1 oncogene signature scores. In SMARCA4 knockout cells, EMT was upregulated, and the cell line transcriptome changed from the SL to the MSL subtype. SMARCA4 knockout cells showed cisplatin resistance and Hippo-YAP/TAZ target gene activation. The YAP1 inhibitor verteporfin suppressed the expression of YAP1 target genes, and decreased cell viability and invasiveness on SMARCA4 knockout cells. SMARCA4 inactivation in TNBC endowed the resistance to cisplatin via EMT activation. The YAP1 inhibitor could become a novel strategy for patients with SMARCA4-inactivated TNBC.
THE BASICS OF QUANTUM TECHNOLOGIES IN GENERAL SECONDARY EDUCATION INSTITUTIONS: CONDITIONALITY AND PROVISION
Authors: Lehka Liudmyla
Published: November 2021

Information about curricula of quantum-oriented content disciplines and training of quantum specialists at different levels of higher education in Ukrainian institutions is systematized. The necessity of propaedeutics of quantum technologies training in institutions of general secondary education is substantiated. Examples of the world and Ukrainian practices of popularization of quantum technologies among students of high school age are given.
MENINGKATKAN HASIL BELAJAR SISWA DENGAN MENGGUNAKAN MODEL PROBLEM BASED LEARNINGI (PBL) PADA MATERI TEKANAN ZAT
Authors: Adjilahu Meyko , Yusuf Muhammad , Abdjul Tirtawaty
Published: November 2021

The Classroom Action Research (CAR) is aimed at improving students learning outcomes by using Problem Based Learning (PBL) model on Substance Pressure material in grade VIIIA of SMP Negeri 1 Dulupi. This Classroom Action Research was carried out from October 2019 to March 2020 in the even semester of 2019-2020 with the number of students who were subject to action as many as 25 people and carried out in 2 cycles. the result of the analysis showed that students learning outcomes from cycle I to cycle II has increased. In cycle I, students’ mastery learning only at 64%, with 16 total students who were completed and 9 students incomplete. After being given action in cycle II, there was an increase to 84%, with 21 total students who were completed and 4 students incomplete. Based on these data, it is concluded that Problem Based Learning (PBL) model can improve students learning outcomes
DEVELOPMENT OF MECHANICAL ENERGY TEACHING AIDS BASED ON ARDUINO UNO MICROCONTROLLER ASSISTED ULTRASONIC SENSORS AND LOAD CELLS
Authors: Matsun Matsun , Boisandi Boisandi , Sari Ira Nofita , Hadiati Soka , Hidayat Deden
Published: November 2021

The study aims to produce a mechanical energy prop by worthy using an Arduino Uno microcontroller assisted by an ultrasonic sensor and a load cell. The research method uses research and development design according to the ADDIE model which consists of 5 stages by analysis, design, development, implementation, and evaluation. The feasibility of mechanical energy props is determined according to experts. The research was carried out in 2021, a limited trial was carried out on students at SMA Negeri 3 Sungai Kakap. Research subjects' responses to the props are also known to use response questionnaires. The validation results of mechanical energy props based on the Arduino Uno microcontroller by two media experts obtained an average score of 95% in the excellent category and by two material expert validators, an average percentage of 97.21% was obtained in the excellent category. The response of research subjects to mechanical energy teaching aids based on the Arduino Uno microcontroller obtained an average score of 87.72% with an excellent category. Overall, mechanical energy teaching aids based on the Arduino Uno microcontroller assisted by an ultrasonic sensor and a load cell have been produced.
Biosorption of Ni2+ and Cr3+ in synthetic sewage: Adsorption capacities of water hyacinth (Eichhornia crassipes)
Authors: Ogbozige Francis James , Nwobu Helen
Published: November 2021

Water hyacinth (Eichhornia crassipes) is an aquatic weed that is causing numerous adverse effects on freshwater bodies. Developing countries are still battling on how to control the growth of this weed without damaging other aquatic lives important to man. Literatures have revealed that most developing countries are still discharging untreated sewage containing heavy metals into waterbodies due to economic and technical constraints in handling conventional methods of treating heavy metals. Hence, the research investigated the possibility of using water hyacinth to adsorb heavy metals (Ni2+ and Cr3+) from sewage before discharging into waterbodies in order to solve two major problems faced in the aquatic environment, at minimal cost. This was achieved by using the said weed (water hyacinth) to treat Ni2+ and Cr3+ solutions prepared in the lab. Results showed that the adsorption process for both ions occurred on heterogeneous surfaces while the mechanism of adsorption followed Pseudo 2nd–order kinetics. The Freundlich, Langmuir and Temkin adsorption capacities for Ni2+ are 19.6925 L/g, 0.7470 L/mg and 1.1093 L/mg respectively while for Cr3+ are 16.814 L/g, 0.7011 L/mg and 0.9623 L/mg respectively. However, the heat of sorption for Ni2+ is 96.906 KJ/mol while that of Cr3+ is 98.749 KJ/mol. Furthermore, FT-IR analysis identified seven functional groups involved in the binding sites with more of hydroxyl group (O–H) from alcohol and carboxylic acid. It was concluded that water hyacinth could be used as a potential bio-adsorbent of metal ions.
Keterampilan Menulis Karangan Persuasi Siswa Dalam Pembelajaran Think Talk Write dengan Media Poster
Authors: Octavia Wilda Ayu Hajar , Satrijono Hari , Hutama Fajar Surya , Haidar Dimas Abdi , Rukmana Linda Purnamasari
Published: November 2021

Writing is a complex skill and is difficult for students to master well. This study aims to determine the effect of the application of Think Talk Write learning models with poster media on the writing skills of persuasion essay from fifth grade elementary school students. This type of research is a quasi-experimental design with a pre-test post-test control group. The sample in this study was VA and V class students of SDN Rambigundam 01 Jember. Collecting data in this study using interviews, tests, and documentation. The data analysis technique of student learning outcomes uses t-test. Based on the results of the t-test on the learning outcomes of students' persuasive essay writing skills, it is known that the value of tcount> ttable, (2.208> 2.0180), so it can be concluded that the hypothesis is accepted that the Think Talk Write learning model through poster media influences the writing skills of persuasion writing in the fifth grade students of SDN Rambigundam Jember 1. The results of the pretest and posttest activities also showed that the increase in the value of writing skills written by students in the experimental class was higher than the increase experienced by the control class. Difference in the increase in the average value experienced by the experimental class and control class by 3,7.
 
Menulis merupakan salah satu keterampilan yang kompleks dan sulit dikuasai siswa dengan baik. Penelitian ini bertujuan untuk mengetahui pengaruh penerapan model pembelajaran Think Talk Writedengan media poster terhadap keterampilan menulis karangan persuasi siswa kelas V SD. Jenis penelitian ini merupakan eksperimen semu dengan desainpre-test post-test control group. Sampel dalam penelitian ini adalah siswa kelas VA dan V B SDN Rambigundam 01 Jember. Pengumpulan data dalam penelitian ini menggunakan metode wawancara, tes, dan dokumentasi. Teknik analisisdata hasil belajar siswa menggunakan uji t-test. Berdasarkan hasil uji-t terhadap hasil belajar keterampilan menulis karangan persuasif siswa, diketahui bahwa nilai thitung>ttabel, (2,208>2,0180), sehingga dapat disimpulkan bahwa hipotesis diterima yaitu model pembelajaran Think Talk Write melalui media poster berpengaruh terhadap keterampilan menulis karangan persuasi pada siswa kelas V SDN Rambigundam 01 Jember. Hasil kegiatan pretest dan posttest juga menunjukan bahwa peningkatan nilai keterampilan menulis karangan siswa di kelas eksperimen lebih tinggi dari pada peningkatan yang dialami kelas kontrol. Selisih peningkatan rata-rata nilai yang dialami kelas eksperimen dan kelas kontrol sebesar 3,7.
Food Waste Management: Suatu Studi Literatur
Authors: Sukismanto Sukismanto , Artha Bhenu
Published: November 2021

Dampak negatif dari pemborosan makanan telah lama dikaitkan dengan hubungan erat antara makanan yang terbuang dan kerawanan pangan, mengingat secara global sepertiga dari makanan yang dihasilkan dari konsumsi manusia telah dibuang. Meskipun penelitian ilmiah dapat menginformasikan praktik pengurangan limbah makanan, sehingga memangkas biaya operasional dan menghemat sumber daya bisnis, operator jasa makanan takut akan potensi kerusakan reputasi yang ditimbulkan sebagai akibat dari pengungkapan data tentang jumlah makanan yang terbuang di tempat mereka. Oleh karena itu, untuk mendapatkan persetujuan dari penyedia jasa makanan maka untuk mengambil bagian dalam penelitian ilmiah tentang limbah makanan bisa menjadi sulit. Sebagian besar penyedia layanan makanan tidak dapat menugaskan anggota staf yang berdedikasi untuk mencatat pemborosan makanan karena kekurangan staf dan sibuk beroperasi pada layanan makanan. Keengganan industri untuk berkolaborasi dengan akademisi, menjelaskan mengapa kehadiran peneliti di dapur restoran dengan tujuan audit limbah makanan tidak selalu ada. Penelitian ini menggunakan metode literature review untuk mengetahui faktor-faktor yang berhubungan dengan pengelolaan limbah makanan. Penelitian ini menggunakan 15 artikel penelitian yang diambil dari database Scopus. Penelitian bertujuan untuk mengetahui faktor-faktor yang berhubungan dengan pengelolaan limbah makanan. Hasil penelitian menunjukkan bahwa terdapat variabel yang berpengaruh terhadap pengelolaan sampah makanan, baik yang berpengaruh, berpengaruh negatif, bahkan tidak berpengaruh. Penelitian lain juga menunjukkan pengaruh pengelolaan limbah makanan terhadap variabel lain, yaitu sirkular bioekonomi.
EFFECT OF ARBUSCULAR MYCORRHIZA ON THE UPTAKE OF NUTRIENTS AND PLANT TOLERANCE TO UNFAVORABLE ENVIRONMENTAL FACTORS
Authors: Guralchuk Zh.z.
Published: November 2021

The modern information concerning the importance of arbuscular mycorrhizal symbiosis in phosphorus and other mineral nutrients uptake by plants both under optimal and stress conditions (drought and salinity) is presented. The influence of mycorrhiza on drought and salinity tolerance of plants are discussed.
INFLUENCE OF INSECTICIDE CRUISER 350 FS AND FUNGICIDE MAXIM XL 035 FS ON VIABILITY AND FUNCTIONAL ACTIVITY OF BACTERIA ACHROMOBACTER ALBUM 1122, THE BIOAGENT OF MICROBIC PREPARATION ALBOBACTERIN
Authors: Тokmakova L.M. , Pyschur I.M. , Sabluk V.T. , Gryschenko O.M.
Published: November 2021

The possibility of application of Albobakterin in combination with protectants of a sugar beet – insecticide Cruiser 350 FS and fungicide MaximXL035FSwas shown.Itwasestablishedthatspecifiedprotectants do not suppress the viability and functional activity of bioagent of the microbial preparation Albobakterin – bacteria Achromobacter album 1122.
INFLUENCE OF MICROBIC PREPARATIONS AND MINERAL FERTILIZERS ON THE DISEASES RESISTANCE AND PRODUCTIVITY OF SOYBEAN CULTIVARS
Authors: Derevyanskyi V.P. , Vlasyuk O.S. , Krutylo D.V. , Kovalevska T.M. , Nadkernychnyyi S.P. , Kopilov E.P.
Published: November 2021

Influence of complex of factors (mineral fertilizers, treatment of seed and crops with microbiological preparations) on plants resistance to diseases and productivity of different soybean cultivars was studied. Compositions, which allow to improve plants growth and development, as well as decrease diseases prevalence and increase productivity and quality of products were selected. The models of soybean cultivars with microorganisms – Glуcine max–Bradyrhizobium japonicum were created.
EFFECT OF POLYMYXOBACTERIN ON PRODUCTIVITY AND QUALITY OF WINTER WHEAT GRAIN IN THE FOREST-STEPPE CONDITIONS OF UKRAINE
Authors: Skarzhinsky V. F. , Broschak I.C. , Fedorchak Yu.T. , Tokmakova L.M.
Published: November 2021

The positive dynamics of Polymyxobaсterin on productivity and quality of winter wheat grains of the Smuglyanka cultivar was observed in the conditions of the western Forest-steppe of Ukraine.
EXOPOLYSACCHARIDE SYNTHESIS PRODUCTIVITY BY BACILLUS MUCILAGINOSUS DEPENDING ON THE NITROGEN SOURCE AND ORIGIN OF INOCULUM
Authors: Malinovska I. M.
Published: November 2021

It was established that Bacillus mucilaginosus C-3 requires introduction of nitrogen sourceinto the culture medium in form of nitrate as themost optimal for maximal accumulation of bacterial biomass and sporulation process. Introduction of the ammonium form was less efficient. It was shown that the intensity of nitrogensource use by B. mucilaginosus is highly depended on the physiological state of the inoculum cells. At sowing of spore inoculum theoptimal concentration of potassium nitrate was2.5 g/l resulting in 1010 cells/ml, while under theuse of vegetative cells the optimal concentrationof potassium nitrate was 1.0 g/l leading to theaccumulation of 109 cells/ml.Use of spore inoculum had ensured themaximum productivity of extracellular polysaccharide synthesis. With the higher number ofB. mucilaginosus subcultures in a vegetativestate the efficiency of the exopolysaccharidesynthesis has decreased: after the second passage — by 20.0 %, after the fourth — by 56.5 %,after the sixth — at 127.8 %. After eighth passage the culture loses its ability to synthesizepolysaccharide, especially on the medium withthe N : C ratio 1 : 3 and resume this ability onlyafter sporulation and the significant increase ofthe N : C ratio.
EVALUATION OF CLADOSPORIUM SOIL MOLDS PATHOGENICITY
Authors: Kravchenko N. O. , Kopylova O. B. , Golovach O. V. , Dmytruk O. M.
Published: November 2021

Verification of soil molds from Cladosporium genus was performed in acute experiments with white mice. LD50 of the studied strains was determined followed by the pathological and bacteriological examinations of the inner organs of laboratory animals. It was revealed that studied Cladosporium strains belong to the group of avirulent microorganisms not capable of invasion of the inner organs of the studied warm-blooded animals and thus can be considered as non-pathogenic.
FUNGOID DISEASES OF GALEGA ORIENTALIS
Authors: Kyrylenko L. V. , Patyka V. P.
Published: November 2021

It was established that the major fungoid diseases of Galeca orientalis of Kavkazkyi branets variety under cultivation in woodland of Ukraine are rust, brown spot, ramularia, cercosporosis. Less commonly, plants are affected by downy mildew, ascochytosis, powdery mildew, gray rot.
CREATION OF NEW DRUG FORMS OF SYMBIOTIC AND ASSOCIATIVE RHIZOBACTERIA WITH AN IMPROVED EFFICIENCY
Authors: Laktionov Y.V. , Kozhemyakov A.P. , Popova T.A.
Published: November 2021

Results of study of the new carrier of microorganisms on the basis of vermikulit, and also influence of humidity, temperatures of storage, various fillers, nutrient elements, stabilizers and other substances on a titer of bacteria in a drug are represented. It is shown that it is possible to keep a high titer of bacteria in a preparation for a long time at an optimum combination of components in the drug.
THE INFLUENCE OF SPECIES OF THE LUPINUS L. GENUS ON THE NUMBER OF RHIZOSPHERIC MICROORGANISMS
Authors: Pyda S.V.
Published: November 2021

The article provides the investigation results of the influence of different species of Lupinus L. genus from three genetic centres on the number of oligonitrophyles, bacteria utilising organic nitrogen, cellulose destructive miсroorganisms, azotobacter, micromyces and streptomyces in the rhizosphere of plants. It was shown that the number of rhizospheric microorganisms depends on the specific peculiarities of plant kinds and sorts.
穴位敷贴治疗良性前列腺增生护理疗效观察
Authors: 凌 晓萍
Published: November 2021

目的:研究良性前列腺增生采用穴位敷贴护理的疗效。 方法:择选 2020 年 01 月至 12 月期间我院收诊的良性前列腺增生患者 120 例,按照收诊先后次序分为两组:观察组和对比组,对比组良性前列腺增生患者在常规治疗+常规护理,而观察组良性前列腺增生患者行常规治疗+常规护理+穴位敷贴,互比两组患者的疗效。 结果:观察组 BPH 患者治疗有效率远高于对比组(P
滋肾活血方联合缬沙坦就早期糖尿病肾病临床疗效评估
Authors: 陈 亮
Published: November 2021

目的:评估滋肾活血方与缬沙坦联合治疗早期糖尿病肾病的疗效。 方法:择取 2019 年 1 月~2021 年 1 月期间本院收治的142 例早期糖尿病肾病患者为对象。 随机均分为对照与观察两组。 对照组予口服缬沙坦,观察组则予缬沙坦联合滋肾活血方治疗。 比对两组治疗后各生化指标及疗效。 结果:观察组 4 项生化指标均明显优于对照组(P
Association between Tea-drinking Habits and Anemia on Pregnant Women in Makassar, Indonesia
Authors: Sundari Sundari , Husnah Nurul , Sharief Suci Avnalurini , Alwi M. Akbar
Published: November 2021

Background: The condition of anemia in pregnant women in Indonesia is exacerbated by the habits of consuming tea. Anemia in pregnant women is still a health problem and a high-level cause of death that causes maternal bleeding indirectly. In Indonesia, tea is the most consumed beverage after water. The aroma of fragrant tea and its distinctive taste make this drink widely consumed. Tea can also be used as an antioxidant, improve skin, and slim the body. However, tea-drinking habits in pregnant women can affect the risk of anemia because tea contains tannins that can inhibit the absorption of iron.
Purpose: To analyze the association between tea-drinking habits and anemia on pregnant women in Makassar, Indonesia.
Methods: This was a quantitative study with cross-sectional design. The subject in this study was 36 pregnant women in the 2nd trimester of pregnancy that was chosen by accidental sampling. The analysis that used was chi-square test to examine the association between tea-drinking habits and anemia. A systematic questionnaire was used to collect data by interviewing respondents.
Result: Of the 36 pregnant women who always drink tea, 22 respondents (48%) had anemia and 14 respondents (30%) were not anemic.  Meanwhile, of 10 respondents who did not have tea consumption habits, 6 respondents (13%) had anemia and 4 respondents (9%) did not suffer from anemia.  Chi-square test showed p=1,000 (p>0,05), which means that there was no association between tea consumption and anemia.
Conclusion: Tea-drinking habit was not related to anemia in pregnant women in Makassar, Indonesia. Pregnant women may consume tea after eating but they need to control the interval, at least one hour after having a meal to avoid anemia.
Features of combined endoscopic tympanoplasty in children with chronic otitis media with cholesteatoma
Authors: Berezniuk Ihor V. , Kovtunenko Oleksandr V. , Berezniuk Volodymyr V.
Published: November 2021

Topicality: Chronic purulent otitis media is a disease that is often encountered in everyday practice by otolaryngologists. To date, the full rehabilitation of the middle ear in pediatric practice has caused much debate about the method of surgery and remains a difficult surgical task.
Aim: to develop a more effective combined method of endoscopic microsurgery in children with chronic otitis media with cholesteatoma.
Materials and methods: examined 52 children aged from 2 to 12 years, operated on a closed variant of tympanoplasty for cholesteatoma otitis. Patients were divided into 2 groups: 1st group (comparison), which used the standard technique of closed variant of surgery with retroauricular incision using an operating microscope (33 patients); and the 2nd group (main), where the combined surgery with retroauricular incision with the using of a microscope and endoscope and endoaural drainage of the antrum was performed in 19 patients.
Results: We conducted an analysis of different types of tympanoplasty in a closed variant of tympanoplasty in children with cholesteatoma otitis and endoaural drainage of the antrum or without it. In the main group, the number of secondary engraftment of the neotympanic membrane decreased 3.4 times, residual perforations – 2.9 times, residual cholesteatoma – 2.9 times, the number of repeated operations – 3.5 times relative to the comparison group.
Conclusion: At the combined surgery of cholesteatoma otitis with endoscopic support at patients of the main group the percent of residual cholesteatoma is lower. The endoaural drainage of the antrum provides additional ventilation and drainage of the reconstructed cavities of a middle ear in the early postoperative period, increases the number of primary engraftment of the neotympanic membrane and reduces the percentage of residual perforations.
The role of bacteriotherapy in the restoration of the nasal mucosa in patients with chronic rhinosinusitis
Authors: Naumenko Alexander N. , Abdelrazzaq Moh’dabdel razzaq Ahmad , Amirkhanova Margaryta R.
Published: November 2021

Topicality: Chronic rhinosinusitis is an polyetiological disease with shch basic complaints as nasal obstruction, nasal discharge, headache or facial pain and reduction of smell. The SNOT-22 questionnaire is widely used to detail patient complaints in various fields. Chronic rhinosinusitis is most often divided into chronic rhinosinusitis with polyps and without nasal polyps. Given the large number of phenotypes and endotypes of the disease, it is important to study further the mechanisms of pathogenesis and selec-
tion of individualized treatment.
Aim: to determine the role of topical application of Streptococcus salivarius 24SMBc and Streptococcus orals 89a in the form
of nasal spray in improving the condition of the nasal mucosa in chronic rhinosinusitis and reducing the recurrence rate.
Materials and methods: The study was conducted at the Department of Otolaryngology of National Medical University on
the basis of KNP "Oleksandrivs’ka Clinical Hospital of Kyiv". It was attended by 82 patients (38 women and 44 men) aged 18 to 60 years, who were randomly divided into two groups. The 1st group (main, 43 patients) received treatment: irrigation therapy with isotonic saline, topical corticosteroid mometasone furoate in combination with a nasal spray, which contains autochthonous saprophytes (AC) – Streptococcus salivarius 89SMBc and Streptococcus orals 89a nasal mucus. The 2nd group (control, 39 patients) received nasal saline irrigation therapy, topical corticosteroid mometasone furoate in combination with topical antibiotic framicetin sulfate. Assessment was performed according to the VAS scale, SNOT-22 questionnaire (before treatment, on the 10th day of treatment and one month after the end of therapy) and determining the frequency of relapses in patients of both groups.
Results: According to the obtained results, the indicators of patients of both groups before treatment and on the 10th day had no statistically significant difference (p>0.05). However a month later patients in the main group had better results (p<0.05) on the scale of VAS and the SNOT-22 questionnaire. So, topical use of Streptococcus salivarius 24SMBc and Streptococcus orals 89a as a nasal spray is effective and can be recommended for the treatment of patients with chronic rhinosinusitis in the acute stage.
Dynamics of changes in the structural components of the mucosa and cartilage of the larynx of rats at the end of 28th and 35th days of experimental opioid exposure
Authors: Ivasivka Khrystyna P. , Paltov Evgeniy V. , Masna Zoryana Z. , Kryvko Yuri Ya. , Cherkes Maryana B.
Published: November 2021

In our work we aimed to conduct a fundamental study of the process of morphological disorganization of the structural components of the mucous membrane and cartilage of the larynx at the end of the 28 and 35 days of experimental opioid effects at the microstructural level.
This information in the future will allow to form a pathomorphological base, which will be used to compare the components of the mucous membrane and cartilage of the larynx in the norm with the dynamics of their changes as a result of experimental opioid effects at different times.
THE WHISTLEBLOWING AS A CATALYST TO SUSTAINABLE ECONOMIC GROWTH AND DEVELOPMENT IN NIGERIA: A CONCEPTUAL REVIEW
Authors: O. Babatunde Sodiq , Lateef Saheed Ademola , D. Olanipekun Wahid , B. Babalola Haorayau
Published: November 2021

Abstract: Whistle-blowing activities around the world have generated huge interests from concerned parties such as the financial and public service, as the aftermath of its neglect often has dilapidating effects on the sustainability of economic growth and development of the nation. This is often seen in the 3rd world nations, such as Nigeria. This study aimed to examine the existing phenomenon between whistle-blowing, economic sustainability, growth and development of Nigeria. This study adopted the Theory of Planned Behavior. However, this was a literature review study that basically draws inferences from studies in this field from different continents (i.e. Europe, America, Asia and Africa). Consequently, the objective of this study was to conceptually investigate the whistle-blowing activities in Nigeria as to how it has assisted in curbing corruption in the public space in turn and improving the economic growth and development. In furtherance, this study reviewed events in African countries with close indexation with Nigeria. Therefore, this study concludes that whistle-blowing has a direct impact on sustainable economic growth and development of Nigeria. This is evident in the Transparency International index report. Lastly, this study recommends that whistleblowing policy should be properly implemented in public institutions to achieve minimum involvement of corruption in the country and that whistleblowers should be protected to encourage disclosure of corrupt acts in the public sector. This in turn will assist in the attainment of sustainable economic growth and development.

Keyword: Whistleblowing, sustainable economic growth, theory of planned behaviour