bluuebunny/crossref_metadata_2025 huggingface dataset:
Long-term survival outcomes in patients with surgically treated oropharyngeal cancer and defined human papilloma virus status
Authors: Dale O T , Sood S , Shah K A , Han C , Rapozo D , Mehanna H , Winter S C
Published: November 2016

Objective:

This study investigated long-term survival outcomes in surgically treated oropharyngeal cancer patients with known human papilloma virus status.

Methods:

A case note review was performed of all patients undergoing primary surgery for oropharyngeal cancer in a single centre over a 10-year period. Human papilloma virus status was determined via dual modality testing. Associations between clinicopathological variables and survival were identified using a log-rank test.

Results:

Of the 107 cases in the study, 40 per cent (n = 41) were human papilloma virus positive. The positive and negative predictive values of p16 immunohistochemistry for human papilloma virus status were 57 per cent and 100 per cent, respectively. At a mean follow up of 59.5 months, 5-year overall and disease-specific survival estimates were 78 per cent and 69 per cent, respectively. Human papilloma virus status (p = 0.014), smoking status (p = 0.021) and tumour stage (p = 0.03) were significant prognostic indicators.

Conclusion:

The long-term survival rates in surgically treated oropharyngeal cancer patients were comparable to other studies. Variables including human papilloma virus status and tumour stage were associated with survival in patients treated with primary surgery; however, nodal stage and presence of extracapsular spread were non-prognostic.
Cannabis-induced attenuated psychotic symptoms: implications for prognosis in young people at ultra-high risk for psychosis
Authors: McHugh M. J. , McGorry P. D. , Yung A. R. , Lin A. , Wood S. J. , Hartmann J. A. , Nelson B.
Published: November 2016

Background

Cannabis use shows a robust dose-dependent relationship with psychosis risk among the general population. Despite this, it has been difficult to link cannabis use with risk for transitioning to a psychotic disorder among individuals at ultra-high risk (UHR) for psychosis. The present study examined UHR transition risk as a function of cannabis use characteristics which vary substantially between individuals including age of first use, cannabis abuse severity and a history of cannabis-induced attenuated psychotic symptoms (APS).

Method

Participants were 190 UHR individuals (76 males) recruited at entry to treatment between 2000 and 2006. They completed a comprehensive baseline assessment including a survey of cannabis use characteristics during the period of heaviest use. Outcome was transition to a psychotic disorder, with mean time to follow-up of 5.0 years (range 2.4–8.7 years).

Results

A history of cannabis abuse was reported in 58% of the sample. Of these, 26% reported a history of cannabis-induced APS. These individuals were 4.90 (95% confidence interval 1.93–12.44) times more likely to transition to a psychotic disorder (p = 0.001). Greater severity of cannabis abuse also predicted transition to psychosis (p = 0.036). However, this effect was mediated by higher abuse severity among individuals with a history of cannabis-induced APS.

Conclusions

Findings suggest that cannabis use poses risk in a subpopulation of UHR individuals who manifest cannabis-induced APS. Whether this reflects underlying genetic vulnerability requires further study. Nevertheless, findings reveal an important early marker of risk with potentially significant prognostic utility for UHR individuals.
N. T. Wright on Paul the Pharisee and ancient Jews in exile
Authors: Mason Steve
Published: November 2016

This article examines two topics that emerge from N. T. Wright's Paul and the Faithfulness of the Gospel: Paul the Shammaite-zealot and the ‘great narrative’ of an Israel in exile, waiting for something. The perspective adopted is that of a historian, for whom the fundamental question is whether Wright's accounts approximate plausible reality two thousand years ago. With respect to the first topic, analysis of source material on the Pharisees in the pre-70 period renders Wright's association of Paul with the rabbinic ‘House of Shammai’ and zealotry doubtful in every part. Similar issues arise in relation to the second topic, where Wright's proposal is supported by a kind of proof-texting, without methodical concern for the nature, context, coherence, themes, rhetoric or meaning of texts in situ.
Differentiated selves help only when identification is strong and tasks are complex
Authors: Nijstad Bernard A. , De Dreu Carsten K. W.
Published: November 2016

Whereas differentiation is overestimated – it more often hurts than helps group performance – identification is underestimated. A more viable perspective sees identification and cooperative motivation as the sine qua non of group functioning, with differentiation helping in a relatively narrow set of cognitively complex tasks that require creativity and deep and deliberate information processing by individual members.
Ecological shifts during larval development in two West African savanna frogs
Authors: Glos Julian , Wolter Johanna , Struck Ulrich , Rödel Mark-Oliver
Published: November 2016

During their ontogeny many organisms exhibit size and/or stage-specific variability in a variety of features related to morphology and ecology, leading to a shift in the ecological niche between size- or stage groups. We analysed such ecological shifts between four larval stage groups in each of two syntopic species of frogs (a total of 5870 individuals) in two West African savanna ponds in Côte d'Ivoire. We hypothesized and confirmed differences between stage groups in ecological variables related to microhabitat and feeding niche. Stage groups differed up to 1‰ in δ15N and 1.5‰ in δ13C isotopic signatures, up to 10 cm in selected water depth, up to 6 m in distance to the pond's edge, and in the coverage of submerged vegetation (range = 10–50%). However, the hypothesis that tadpoles with generalized mouthparts (Kassina spp.) exhibit more pronounced ontogenetic shifts in food-web position than a specialized filter-feeding species (Phrynomantis microps), and that differences between larval stages are more pronounced in a heterogeneous habitat (offering more choices of habitat and food niches) than in a homogeneous habitat (offering less choices) was only partly confirmed. This study underlines that potential niche shift during different ontogenetic stages is an important factor to be considered in ecological studies.
Avian predators avoid attacking artificial aposematic millipedes in Brazilian Atlantic Forest
Authors: Iniesta Luiz F. M. , Ratton Pedro , Guerra Tadeu J.
Published: November 2016

Avian predators may represent an important selective pressure favouring the evolution of aposematic colouration in millipedes that present chemical defences. However, the role of their warning colouration in predator avoidance remains poorly explored under field conditions, especially in Neotropical forests. Thus, to evaluate the hypothesis of the predator avoidance, we conducted a factorial experiment utilizing artificial replicas of millipedes constructed from plasticine and placed in the natural habitat of Odontopeltis aleijadinho (Polydesmida, Chelodesmidae), an Atlantic Forest endemic aposematic millipede. We assessed patterns of attack to aposematic and non-aposematic replicas applied with repugnant fluid extracted from living millipedes and two control treatments, distributed as a total of 300 replicas exposed for 48 h on the forest floor. The average percentage of replicas attacked was nearly 10-fold higher on those non-aposematic control replicas (13.3% ± 3.3%) than on aposematic replicas (1.3% ± 0.9%). In 24 replicas attacked by avian predators, no effect of millipede repugnant fluid was found, but the effect of colouration pattern was statistically significant. Our data support the hypothesis that a warning colouration pattern involving yellow spots symmetrically distributed along the millipede body can mediate avian predator avoidance.
Characterizing the risk of respiratory syncytial virus in infants with older siblings: a population-based birth cohort study
Authors: JACOBY P. , GLASS K. , MOORE H. C.
Published: November 2016

From a population-based birth cohort of 245 249 children born in Western Australia during 1996–2005, we used linkage of laboratory and birth record datasets to obtain data including all respiratory syncytial virus (RSV) detections during infancy from a subcohort of 87 981 singleton children born in the Perth metropolitan area from 2000 to 2004. Using log binomial regression, we found that the risk of infant RSV detection increases with the number of older siblings, with those having ⩾3 older siblings experiencing almost three times the risk (relative risk 2·83, 95% confidence interval 2·46–3·26) of firstborn children. We estimate that 45% of the RSV detections in our subcohort were attributable to infection from an older sibling. The sibling effect was significantly higher for those infants who were younger during the season of peak risk (winter) than those who were older. Although older siblings were present in our cohort, they had very few RSV detections which could be temporally linked to an infant's infection. We conclude that RSV infection in older children leads to less severe symptoms but is nevertheless an important source of infant infection. Our results lend support to a vaccination strategy which includes family members in order to provide maximum protection for newborn babies.
Nutritional management of hyperapoB
Authors: Lamantia Valérie , Sniderman Allan , Faraj May
Published: November 2016

Plasma apoB is a more accurate marker of the risk of CVD and type 2 diabetes (T2D) than LDL-cholesterol; however, nutritional reviews targeting apoB are scarce. Here we reviewed eighty-seven nutritional studies and present conclusions in order of strength of evidence. Plasma apoB was reduced in all studies that induced weight loss of 6–12 % using hypoenergetic diets (seven studies; 5440–7110 kJ/d; 1300–1700 kcal/d; 34–50 % carbohydrates; 27–39 % fat; 18–24 % protein). When macronutrients were compared in isoenergetic diets (eleven studies including eight randomised controlled trials (RCT);n1189), the diets that reduced plasma apoB were composed of 26–51 % carbohydrates, 26–46 % fat, 11–32 % protein, 10–27 % MUFA, 5–14 % PUFA and 7–13 % SFA. Replacement of carbohydrate by MUFA, not SFA, decreased plasma apoB. Moreover, dietary enriching withn-3 fatty acids (FA) (from fish: 1·1–1·7 g/d or supplementation: 3·2–3·4 g/d EPA/DHA or 4 g/d EPA), psyllium (about 8–20 g/d), phytosterols (about 2–4 g/d) or nuts (30–75 g/d) also decreased plasma apoB, mostly in hyperlipidaemic subjects. While high intake oftrans-FA (4·3–9·1 %) increased plasma apoB, it is unlikely that these amounts represent usual consumption. Inconsistent data existed on the effect of soya proteins (25–30 g/d), while the positive association of alcohol consumption with low plasma apoB was reported in cross-sectional studies only. Five isoenergetic studies using Mediterranean diets (including two RCT; 823 subjects) reported a decrease of plasma apoB, while weaker evidence existed for Dietary Approaches to Stop Hypertension (DASH), vegetarian, Nordic and Palaeolithic diets. We recommend using a Mediterranean dietary pattern, which also encompasses the dietary components reported to reduce plasma apoB, to target hyperapoB and reduce the risks of CVD and T2D.
The association between pro-arrhythmic agents and aortic stenosis in young adults: is it sufficient to clarify the sudden unexpected deaths?
Authors: Radnic Bojana , Radojevic Nemanja , Vucinic Jelena , Duborija-Kovacevic Natasa
Published: November 2016

Most young patients with mild-to-moderate aortic stenosis show no symptoms, and sudden death appears only occasionally. We hypothesised that malignant ventricular arrhythmias could be responsible for the high incidence of sudden death in such patients. If multiple factors such as asymptomatic aortic stenosis in association with arrhythmia-provoking agents are involved, could it be sufficient to account for sudden unexpected death? In this study, eight cases of sudden death in young adults, with ages ranging from 22 to 36 years, who had never reported any symptoms that could be related to aortic stenosis, were investigated. Full autopsies were performed, and congenital aortic stenosis in all eight cases was confirmed. DNA testing for channelopathies was negative. Comprehensive toxicological analyses found an electrolyte imbalance, or non-toxic concentrations of amitriptyline, terfenadine, caffeine, and ethanol. Collectively, these results suggest that congenital asymptomatic aortic stenosis without cardiac hypertrophy in young adults is not sufficient to cause sudden death merely on its own; rather, an additional provoking factor is necessary. According to our findings, the provoking factor may be a state of physical or emotional stress, a state of electrolyte imbalance, or even taking a therapeutic dose of a particular drug.
Asenapine for the treatment of adults with an acute exacerbation of schizophrenia: results from a randomized, double-blind, fixed-dose, placebo-controlled trial with olanzapine as an active control
Authors: Landbloom Ronald , Mackle Mary , Wu Xiao , Kelly Linda , Snow-Adami Linda , McIntyre Roger S. , Mathews Maju , Hundt Carla
Published: November 2016

Objective

Evaluate the efficacy and safety of asenapine 2.5 mg twice daily (bid; n=97) or 5 mg bid (n=113) versus placebo (n=101) in adults with acute exacerbation of schizophrenia.

Methods

Adults with Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition, Text Revision (DSM-IV-TR) schizophrenia diagnosis were randomized to asenapine 2.5 mg bid, 5 mg bid, placebo, or olanzapine 15 mg once daily. The primary objective was to test superiority of asenapine versus placebo as measured by the change from baseline to day 42 in the Positive and Negative Syndrome Scale (PANSS) total score. The key safety objective was to evaluate weight change in asenapine versus olanzapine at day 42.

Results

The primary efficacy endpoint was met; the difference in least squares mean change from baseline to day 42 in PANSS total score between asenapine 5 mg bid and placebo was −5.5 points (unadjusted 95% CI: −10.1, −1.0; multiplicity adjusted P=0.0356). Neither asenapine 2.5 mg bid nor olanzapine 15mg were superior to placebo.

Both asenapine groups demonstrated significantly less weight gain than olanzapine at day 42. Significantly higher incidences of oral hypoesthesia and dysgeusia (combined) for asenapine 2.5 mg bid (5.2% vs 0.0%; P=0.0217) and 5 mg bid (7.1% vs 0.0%; P=0.0033) were observed versus placebo. There were no significant differences between asenapine and placebo for insomnia, extrapyramidal symptoms, akathisia, dizziness, or combination of somnolence/sedation/hypersomnia.

Conclusion

This study supports previous efficacy and safety findings of asenapine; asenapine 5 mg bid is the lowest effective dose in adults with schizophrenia. Asenapine was associated with significantly less weight gain than olanzapine at day 42.
Perinatal, sociodemographic and lifestyle correlates of increased total and visceral fat mass levels in schoolchildren in Greece: the Healthy Growth Study
Authors: Moschonis George , Kaliora Adriana C , Karatzi Kalliopi , Michaletos Aggelos , Lambrinou Christina-Paulina , Karachaliou Alexandra K , Chrousos George P , Lionis Christos , Manios Yannis
Published: November 2016

Objective

To identify possibly independent associations of perinatal, sociodemographic and lifestyle factors with childhood total and visceral body fat.

Design

A representative sample of 2655 schoolchildren (9–13 years) participated in the Healthy Growth Study, a cross-sectional epidemiological study.

Setting

Seventy-seven primary schools in four large regions in Greece.

Subjects

A sample of 1228 children having full data on total and visceral fat mass levels, as well as on anthropometric, dietary, physical activity, physical examination, socio-economic and perinatal indices, was examined.

Results

Maternal (OR=3·03 and 1·77) and paternal obesity (OR=1·62 and 1·78), maternal smoking during pregnancy (OR=1·72 and 1·93) and rapid infant weight gain (OR=1·42 and 1·96) were significantly and positively associated with children’s increased total and visceral fat mass levels, respectively. Children’s television watching for >2 h/d (OR=1·40) and maternal pre-pregnancy obesity (OR=2·46) were associated with children’s increased total and visceral fat mass level, respectively. Furthermore, increased children’s physical activity (OR=0·66 and 0·47) were significantly and negatively associated with children’s total and visceral fat mass levels, respectively. Lastly, both father’s age >46 years (OR=0·57) and higher maternal educational level (OR=0·45) were associated with children’s increased total visceral fat mass level.

Conclusions

Parental sociodemographic characteristics, perinatal indices and pre-adolescent lifestyle behaviours were associated with children’s abnormal levels of total and visceral fat mass. Any future programme for childhood prevention either from the perinatal age or at late childhood should take these indices into consideration.
CURRENT-MODIFIED EVOLUTION EQUATION FOR A BROADER BANDWIDTH CAPILLARY–GRAVITY WAVE PACKET
Authors: DEBSARMA SUMA , DAS K. P.
Published: November 2016

We derive a higher order nonlinear evolution equation for a broader bandwidth three-dimensional capillary–gravity wave packet, in the presence of a surface current produced by an internal wave. Instead of a set of coupled equations, a single nonlinear evolution equation is obtained by eliminating the velocity potential for the wave-induced slow motion. Finally, the equation is expressed in an integro-differential equation form, similar to Zakharov’s integral equation. Using the evolution equation derived here, we show that the two sidebands of a surface capillary–gravity wave get excited as a result of resonance with an internal wave, all propagating in the same direction. It is also shown that surface waves can grow exponentially with time at the expense of the energy of the internal wave.
Effects of supplementation of a forage-only diet with wheat bran and sugar beet pulp in organic dairy cows
Authors: Ertl Paul , Zebeli Qendrim , Zollitsch Werner , Knaus Wilhelm
Published: November 2016

Although levels of concentrate supplementation are generally lower in organic as compared with conventional dairy cows, forage-only (FO) diets are not very common in organic dairy cows because of the resulting limited dry matter intake (DMI) and lower milk production. However, from the perspective of net food production, FO diets or forage diets supplemented only with by-products from the food processing industry, offer considerable potential because they do not compete with humans for food. The aim of the present study was therefore to investigate the effects of adding a mixture of wheat bran and dried sugar beet pulp [0.56:0.44 on a dry matter (DM) basis] to a FO diet on DMI, milk production, chewing activity and production efficiency. Seventeen multiparous and three primiparous mid-lactation Holstein cows were randomly assigned to one of two treatments, receiving either a FO mixture with hay and grass silage in equal proportions (FO) or the same forage mixture supplemented with a mixture of wheat bran and dried sugar beet pulp at a rate of 25% of dietary DM (25%BP). The experiment was conducted in a change-over design with two experimental periods of 7 and 6 weeks, respectively. Overall, feeding the 25%BP diet increased DMI and energy-corrected milk (ECM) yield by 1.8 kg d−1 as compared with cows fed FO. Feed conversion efficiency (kg ECM per kg DMI) and energy efficiency (kg ECM per 10 MJ net energy for lactation intake) were higher in FO, but cows fed FO were in a slightly negative energy balance and also tended to have a higher mobilization of body tissues as compared with cows fed 25%BP. In comparison with FO, cows receiving 25%BP showed less chewing activity per kg DMI or per kg neutral detergent fiber ingested. In conclusion, results from this feeding trial showed that adding wheat bran and dried sugar beet pulp to a FO diet increased DMI and milk yield and improved the energy balance when compared with a FO diet, although the magnitude of the milk yield response was lower than expected.
Working Alliance Inventory for Children and Adolescents	(WAI-CA)
Authors: Figueiredo Bárbara , Dias Pedro , Lima Vânia Sousa , Lamela Diogo
Published: November 2016

Abstract. The purpose of this study was to validate a version of the Working Alliance Inventory (WAI) for children and adolescents (WAI-CA). The sample included 109 children/adolescents aged between 7 and 17 years, outpatients in a Clinical Psychology Unit (Portugal), who completed the WAI-CA between psychotherapy sessions 3 and 35. A subsample of 30 children/adolescents aged between 10 and 14 years filled out both the WAI-CA and the WAI within a one-to-two week’s interval. A subsample of 57 children/adolescents with ages between 7 and 17 years filled out the WAI-CA, and their accompanying parent the WAI. Results show high internal consistency (Cronbach’s alpha ranging from .71 to .89) and good external validity. Significant differences were found in the bond subscale according to age, gender, and diagnosis, with higher values in children compared to adolescents, in girls compared to boys, and in participants with internalizing and externalizing problems compared to participants with school problems. Moderate to strong significant correlations were found between children/adolescents’ WAI-CA and WAI scores and weak correlations between children/adolescents’ WAI-CA scores and parent’s WAI scores. Results suggest that the WAI-CA is a valid measure of working alliance to be used with children and adolescents.
Assessing Personality With Multi-Descriptor Items
Authors: Schult Johannes , Schneider Rebecca , Sparfeldt Jörn R.
Published: November 2016

Abstract. The need for efficient personality inventories has led to the wide use of short instruments. The corresponding items often contain multiple, potentially conflicting descriptors within one item. In Study 1 ( N = 198 university students), the reliability and validity of the TIPI (Ten-Item Personality Inventory) was compared with the reliability and validity of a modified TIPI based on items that rephrased each two-descriptor item into two single-descriptor items. In Study 2 ( N = 268 university students), we administered the BFI-10 (Big Five Inventory short version) and a similarly modified version of the BFI-10 without two-descriptor items. In both studies, reliability and construct validity values occasionally improved for separated multi-descriptor items. The inventories with multi-descriptor items showed shortcomings in some factors of the TIPI and the BFI-10. However, the other scales worked comparably well in the original and modified inventories. The limitations of short personality inventories with multi-descriptor items are discussed.
Detecting Random Responses in a Personality Scale Using IRT-Based	Person-Fit Indices
Authors: Liu Tour , Lan Tian , Xin Tao
Published: November 2016

Abstract. Random response is a very common aberrant response behavior in personality tests and may negatively affect the reliability, validity, or other analytical aspects of psychological assessment. Typically, researchers use a single person-fit index to identify random responses. This study recommends a three-step person-fit analysis procedure. Unlike the typical single person-fit methods, the three-step procedure identifies both global misfit and local misfit individuals using different person-fit indices. This procedure was able to identify more local misfit individuals than single-index method, and a graphical method was used to visualize those particular items in which random response behaviors appear. This method may be useful to researchers in that it will provide them with more information about response behaviors, allowing better evaluation of scale administration and development of more plausible explanations. Real data were used in this study instead of simulation data. In order to create real random responses, an experimental test administration was designed. Four different random response samples were produced using this experimental system.
The Effect of Alternative Scoring Procedures on the Measurement	Properties of a Self-Administered Depression Scale
Authors: Iwata Noboru , Tsutsumi Akizumi , Wakita Takafumi , Kumagai Ryuichi , Noguchi Hiroyuki , Watanabe Naotaka
Published: November 2016

Abstract. To investigate the effect of response alternatives/scoring procedures on the measurement properties of the Center for Epidemiologic Studies Depression Scale (CES-D) which has the four response alternatives, a polytomous item response theory (IRT) model was applied to the responses of 2,061 workers and university students (1,640 males, 421 females). Test information functions derived from the polytomous IRT analyses on the CES-D data with various scoring procedures indicated that: (1) the CES-D with its standard (0-1-2-3) scoring procedure should be useful for screening to detect subjects with “at high-risk” of depression if the θ point showing the highest information corresponds to the cut-off point, because of its extremely higher information; (2) the CES-D with the 0-1-1-2 scoring procedure could cover wider range of depressive severity, suggesting that this scoring procedure might be useful in cases where more exhaustive discrimination in symptomatology is of interest; and (3) the revised version of CES-D with replacing original positive items into negatively revised items outperformed the original version. These findings have never been demonstrated by the classical test theory analyses, and thus the utility of this kind of psychometric testing should be warranted to further investigation for the standard measures of psychological assessment.
Ancient and methane-derived carbon subsidizes contemporary food webs
Authors: DelVecchia Amanda G. , Stanford Jack A. , Xu Xiaomei
Published: November 2016

While most global productivity is driven by modern photosynthesis, river ecosystems are supplied by locally fixed and imported carbon that spans a range of ages. Alluvial aquifers of gravel-bedded river floodplains present a conundrum: despite no possibility for photosynthesis in groundwater and extreme paucity of labile organic carbon, they support diverse and abundant large-bodied consumers (stoneflies, Insecta: Plecoptera). Here we show that up to a majority of the biomass carbon composition of these top consumers in four floodplain aquifers of Montana and Washington is methane-derived. The methane carbon ranges in age from modern to up to >50,000 years old and is mostly derived from biogenic sources, although a thermogenic contribution could not be excluded. We document one of the most expansive ecosystems to contain site-wide macroinvertebrate biomass comprised of methane-derived carbon and thereby advance contemporary understanding of basal resources supporting riverine productivity.
Structure of the ribosome post-recycling complex probed by chemical cross-linking and mass spectrometry
Authors: Kiosze-Becker Kristin , Ori Alessandro , Gerovac Milan , Heuer André , Nürenberg-Goloub Elina , Rashid Umar Jan , Becker Thomas , Beckmann Roland , Beck Martin , Tampé Robert
Published: November 2016

Ribosome recycling orchestrated by the ATP binding cassette (ABC) protein ABCE1 can be considered as the final—or the first—step within the cyclic process of protein synthesis, connecting translation termination and mRNA surveillance with re-initiation. An ATP-dependent tweezer-like motion of the nucleotide-binding domains in ABCE1 transfers mechanical energy to the ribosome and tears the ribosome subunits apart. The post-recycling complex (PRC) then re-initiates mRNA translation. Here, we probed the so far unknown architecture of the 1-MDa PRC (40S/30S·ABCE1) by chemical cross-linking and mass spectrometry (XL-MS). Our study reveals ABCE1 bound to the translational factor-binding (GTPase) site with multiple cross-link contacts of the helix–loop–helix motif to the S24e ribosomal protein. Cross-linking of the FeS cluster domain to the ribosomal protein S12 substantiates an extreme lever-arm movement of the FeS cluster domain during ribosome recycling. We were thus able to reconstitute and structurally analyse a key complex in the translational cycle, resembling the link between translation initiation and ribosome recycling.
Pupil fluctuations track rapid changes in adrenergic and cholinergic activity in cortex
Authors: Reimer Jacob , McGinley Matthew J , Liu Yang , Rodenkirch Charles , Wang Qi , McCormick David A , Tolias Andreas S
Published: November 2016

Rapid variations in cortical state during wakefulness have a strong influence on neural and behavioural responses and are tightly coupled to changes in pupil size across species. However, the physiological processes linking cortical state and pupil variations are largely unknown. Here we demonstrate that these rapid variations, during both quiet waking and locomotion, are highly correlated with fluctuations in the activity of corticopetal noradrenergic and cholinergic projections. Rapid dilations of the pupil are tightly associated with phasic activity in noradrenergic axons, whereas longer-lasting dilations of the pupil, such as during locomotion, are accompanied by sustained activity in cholinergic axons. Thus, the pupil can be used to sensitively track the activity in multiple neuromodulatory transmitter systems as they control the state of the waking brain.
Genome sequence and analysis of the Japanese morning glory Ipomoea nil
Authors: Hoshino Atsushi , Jayakumar Vasanthan , Nitasaka Eiji , Toyoda Atsushi , Noguchi Hideki , Itoh Takehiko , Shin-I Tadasu , Minakuchi Yohei , Koda Yuki , Nagano Atsushi J. , Yasugi Masaki , Honjo Mie N. , Kudoh Hiroshi , Seki Motoaki , Kamiya Asako , Shiraki Toshiyuki , Carninci Piero , Asamizu Erika , Nishide Hiroyo , Tanaka Sachiko , Park Kyeung-Il , Morita Yasumasa , Yokoyama Kohei , Uchiyama Ikuo , Tanaka Yoshikazu , Tabata Satoshi , Shinozaki Kazuo , Hayashizaki Yoshihide , Kohara Yuji , Suzuki Yutaka , Sugano Sumio , Fujiyama Asao , Iida Shigeru , Sakakibara Yasubumi
Published: November 2016

Ipomoeais the largest genus in the family Convolvulaceae.Ipomoea nil(Japanese morning glory) has been utilized as a model plant to study the genetic basis of floricultural traits, with over 1,500 mutant lines. In the present study, we have utilized second- and third-generation-sequencing platforms, and have reported a draft genome ofI. nilwith a scaffold N50 of 2.88 Mb (contig N50 of 1.87 Mb), covering 98% of the 750 Mb genome. Scaffolds covering 91.42% of the assembly are anchored to 15 pseudo-chromosomes. The draft genome has enabled the identification and cataloguing of theTpn1family transposons, known as the major mutagen ofI. nil, and analysing the dwarf gene,CONTRACTED, located on the genetic map published in 1956. Comparative genomics has suggested that a whole genome duplication in Convolvulaceae, distinct from the recent Solanaceae event, has occurred after the divergence of the two sister families.
Magnetic slippery extreme icephobic surfaces
Authors: Irajizad Peyman , Hasnain Munib , Farokhnia Nazanin , Sajadi Seyed Mohammad , Ghasemi Hadi
Published: November 2016

Anti-icing surfaces have a critical footprint on daily lives of humans ranging from transportation systems and infrastructure to energy systems, but creation of these surfaces for low temperatures remains elusive. Non-wetting surfaces and liquid-infused surfaces have inspired routes for the development of icephobic surfaces. However, high freezing temperature, high ice adhesion strength, and high cost have restricted their practical applications. Here we report new magnetic slippery surfaces outperforming state-of-the-art icephobic surfaces with a ice formation temperature of −34 °C, 2–3 orders of magnitude higher delay time in ice formation, extremely low ice adhesion strength (≈2 Pa) and stability in shear flows up to Reynolds number of 105. In these surfaces, we exploit the magnetic volumetric force to exclude the role of solid–liquid interface in ice formation. We show that these inexpensive surfaces are universal and can be applied to all types of solids (no required micro/nano structuring) with no compromise to their unprecedented properties.
Hybrid mass spectrometry approaches in glycoprotein analysis and their usage in scoring biosimilarity
Authors: Yang Yang , Liu Fan , Franc Vojtech , Halim Liem Andhyk , Schellekens Huub , Heck Albert J. R.
Published: November 2016

Many biopharmaceutical products exhibit extensive structural micro-heterogeneity due to an array of co-occurring post-translational modifications. These modifications often effect the functionality of the product and therefore need to be characterized in detail. Here, we present an integrative approach, combining two advanced mass spectrometry-based methods, high-resolution native mass spectrometry and middle-down proteomics, to analyse this micro-heterogeneity. Taking human erythropoietin and the human plasma properdin as model systems, we demonstrate that this strategy bridges the gap between peptide- and protein-based mass spectrometry platforms, providing the most complete profiling of glycoproteins. Integration of the two methods enabled the discovery of three undescribed C-glycosylation sites on properdin, and revealed in addition unexpected heterogeneity in occupancies of C-mannosylation. Furthermore, using various sources of erythropoietin we define and demonstrate the usage of a biosimilarity score to quantitatively assess structural similarity, which would also be beneficial for profiling other therapeutic proteins and even plasma protein biomarkers.
Combining social cognitive treatment, cognitive remediation, and functional skills training in schizophrenia: a randomized controlled trial
Authors: Peña Javier , Ibarretxe-Bilbao Naroa , Sánchez Pedro , Iriarte Maria B , Elizagarate Edorta , Garay Maria A , Gutiérrez Miguel , Iribarren Aránzazu , Ojeda Natalia
Published: November 2016

This study examined the efficacy of an integrative cognitive remediation program (REHACOP) in improving cognition and functional outcome in patients with schizophrenia. The program combines cognitive remediation, social cognitive intervention, and functional skills training. Few studies have attempted this approach. One hundred and eleven patients diagnosed with schizophrenia were randomly assigned to either the cognitive remediation group (REHACOP) or an active control group (occupational activities) for 4 months (three sessions per week, 90 min). Primary outcomes were change on general neurocognitive performance and social cognition, including theory of mind (ToM), emotion perception (EP), attributional style, and social perception (SP). Secondary outcomes included changes on clinical symptoms (Positive and Negative Syndrome Scale) and functional outcome (UCSD Performance-Based Skills Assessment and the Global Assessment of Functioning). The trial was registered with clinicaltrials.gov (NCT02796417). No baseline group differences were found. Significant differences were found in the mean change between the REHACOP group and control group in neurocognition (

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.
148

), EP (

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136

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.
154

). The combination of cognitive remediation, social cognitive intervention, and functional skills training demonstrated statistically significant and clinically meaningful changes in neurocognition, social cognition, negative, and functional disability.
Gene-gene Interaction Analyses for Atrial Fibrillation
Authors: Lin Honghuang , Mueller-Nurasyid Martina , Smith Albert V. , Arking Dan E. , Barnard John , Bartz Traci M. , Lunetta Kathryn L. , Lohman Kurt , Kleber Marcus E. , Lubitz Steven A. , Geelhoed Bastiaan , Trompet Stella , Niemeijer Maartje N. , Kacprowski Tim , Chasman Daniel I. , Klarin Derek , Sinner Moritz F. , Waldenberger Melanie , Meitinger Thomas , Harris Tamara B. , Launer Lenore J. , Soliman Elsayed Z. , Chen Lin Y. , Smith Jonathan D. , Van Wagoner David R. , Rotter Jerome I. , Psaty Bruce M. , Xie Zhijun , Hendricks Audrey E. , Ding Jingzhong , Delgado Graciela E. , Verweij Niek , van der Harst Pim , Macfarlane Peter W. , Ford Ian , Hofman Albert , Uitterlinden André , Heeringa Jan , Franco Oscar H. , Kors Jan A. , Weiss Stefan , Völzke Henry , Rose Lynda M. , Natarajan Pradeep , Kathiresan Sekar , Kääb Stefan , Gudnason Vilmundur , Alonso Alvaro , Chung Mina K. , Heckbert Susan R. , Benjamin Emelia J. , Liu Yongmei , März Winfried , Rienstra Michiel , Jukema J. Wouter , Stricker Bruno H. , Dörr Marcus , Albert Christine M. , Ellinor Patrick T.
Published: November 2016

Atrial fibrillation (AF) is a heritable disease that affects more than thirty million individuals worldwide. Extensive efforts have been devoted to the study of genetic determinants of AF. The objective of our study is to examine the effect of gene-gene interaction on AF susceptibility. We performed a large-scale association analysis of gene-gene interactions with AF in 8,173 AF cases, and 65,237 AF-free referents collected from 15 studies for discovery. We examined putative interactions between genome-wide SNPs and 17 known AF-related SNPs. The top interactions were then tested for association in an independent cohort for replication, which included more than 2,363 AF cases and 114,746 AF-free referents. One interaction, between rs7164883 at the HCN4 locus and rs4980345 at the SLC28A1 locus, was found to be significantly associated with AF in the discovery cohorts (interaction OR = 1.44, 95% CI: 1.27–1.65, P = 4.3 × 10–8). Eight additional gene-gene interactions were also marginally significant (P < 5 × 10–7). However, none of the top interactions were replicated. In summary, we did not find significant interactions that were associated with AF susceptibility. Future increases in sample size and denser genotyping might facilitate the identification of gene-gene interactions associated with AF.
Auxin flow-mediated competition between axillary buds to restore apical dominance
Authors: Balla Jozef , Medveďová Zuzana , Kalousek Petr , Matiješčuková Natálie , Friml Jiří , Reinöhl Vilém , Procházka Stanislav
Published: November 2016

Apical dominance is one of the fundamental developmental phenomena in plant biology, which determines the overall architecture of aerial plant parts. Here we show apex decapitation activated competition for dominance in adjacent upper and lower axillary buds. A two-nodal-bud pea (Pisum sativum L.) was used as a model system to monitor and assess auxin flow, auxin transport channels, and dormancy and initiation status of axillary buds. Auxin flow was manipulated by lateral stem wounds or chemically by auxin efflux inhibitors 2,3,5-triiodobenzoic acid (TIBA), 1-N-naphtylphtalamic acid (NPA), or protein synthesis inhibitor cycloheximide (CHX) treatments, which served to interfere with axillary bud competition. Redirecting auxin flow to different points influenced which bud formed the outgrowing and dominant shoot. The obtained results proved that competition between upper and lower axillary buds as secondary auxin sources is based on the same auxin canalization principle that operates between the shoot apex and axillary bud.
Synthesis and Characterization of Superhydrophobic, Self-cleaning NIR-reflective Silica Nanoparticles
Authors: Sriramulu Deepa , Reed Ella Louise , Annamalai Meenakshi , Venkatesan Thirumalai Venky , Valiyaveettil Suresh
Published: November 2016

Multifunctional coatings offer many advantages towards protecting various surfaces. Here we apply aggregation induced segregation of perylene diimide (PDI) to control the surface morphology and properties of silica nanoparticles. Differentially functionalized PDI was incorporated on the surface of silica nanoparticles through Si-O-Si bonds. The absorption and emission spectra of the resultant functionalised nanoparticles showed monomeric or excimeric peaks based on the amounts of perylene molecules present on the surface of silica nanoparticles. Contact angle measurements on thin films prepared from nanoparticles showed that unfunctionalised nanoparticles were superhydrophilic with a contact angle (CA) of 0°, whereas perylene functionalised silica particles were hydrophobic (CA > 130°) and nanoparticles functionalised with PDI and trimethoxy(octadecyl)silane (TMODS) in an equimolar ratio were superhydrophobic with static CA > 150° and sliding angle (SA) < 10°. In addition, the near infrared (NIR) reflectance properties of PDI incorporated silica nanoparticles can be used to protect various heat sensitive substrates. The concept developed in this paper offers a unique combination of super hydrophobicity, interesting optical properties and NIR reflectance in nanosilica, which could be used for interesting applications such as surface coatings with self-cleaning and NIR reflection properties.
Intake of 7,8-Dihydroxyflavone During Juvenile and Adolescent Stages Prevents Onset of Psychosis in Adult Offspring After Maternal Immune Activation
Authors: Han Mei , Zhang Ji-chun , Yao Wei , Yang Chun , Ishima Tamaki , Ren Qian , Ma Min , Dong Chao , Huang Xu-Feng , Hashimoto Kenji
Published: November 2016

Prenatal infection and subsequent abnormal neurodevelopment of offspring is involved in the etiology of schizophrenia. Brain-derived neurotrophic factor (BDNF) and its high affinity receptor, tropomyosin receptor kinase B (TrkB) signaling plays a key role in the neurodevelopment. Pregnant mice exposed to polyriboinosinic-polyribocytidylic acid [poly(I:C)] causes schizophrenia-like behavioral abnormalities in their offspring at adulthood. Here we found that the juvenile offspring of poly(I:C)-treated mice showed cognitive deficits, as well as reduced BDNF-TrkB signaling in the prefrontal cortex (PFC). Furthermore, the adult offspring of poly(I:C)-treated mice showed cognitive deficits, prepulse inhibition (PPI) deficits, reduced BDNF-TrkB signaling, immunoreactivity of parvalbumin (PV) and peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α) in the prelimbic (PrL) of medial PFC and CA1 of hippocampus. Supplementation of a TrkB agonist 7,8-dihydroxyflavone (1 mg/mL in drinking water) during juvenile and adolescent stages could prevent these behavioral abnormalities, reduced BDNF-TrkB signaling in PFC and CA1, and immunoreactivity of PV and PGC-1α in the PrL of medial PFC and CA1 in the adult offspring from poly(I:C)-treated mice. These findings suggest that early intervention by a TrkB agonist in subjects with ultra-high risk for psychosis may reduce the risk of subsequent transition to schizophrenia.
Trafficking of the exported P. falciparum chaperone PfHsp70x
Authors: Rhiel Manuel , Bittl Verena , Tribensky Anke , Charnaud Sarah C. , Strecker Maja , Müller Sebastian , Lanzer Michael , Sanchez Cecilia , Schaeffer-Reiss Christine , Westermann Benoit , Crabb Brendan S. , Gilson Paul R. , Külzer Simone , Przyborski Jude M.
Published: November 2016

Plasmodium falciparum extensively modifies its chosen host cell, the mature human erythrocyte. This remodelling is carried out by parasite-encoded proteins that are exported into the host cell. To gain access to the human red blood cell, these proteins must cross the parasitophorous vacuole, a membrane bound compartment surrounding the parasite that is generated during the invasion process. Many exported proteins carry a so-called PEXEL/HT signal that directs their transport. We recently reported the unexpected finding of a species-restricted parasite-encoded Hsp70, termed PfHsp70x, which is exported into the host erythrocyte cytosol. PfHsp70x lacks a classical PEXEL/HT motif, and its transport appears to be mediated by a 7 amino acid motif directly following the hydrophobic N-terminal secretory signal. In this report, we analyse this short targeting sequence in detail. Surprisingly, both a reversed and scrambled version of the motif retained the capacity to confer protein export. Site directed mutagenesis of glutamate residues within this region leads to a block of protein trafficking within the lumen of the PV. In contrast to PEXEL-containing proteins, the targeting signal is not cleaved, but appears to be acetylated. Furthermore we show that, like other exported proteins, trafficking of PfHsp70x requires the vacuolar translocon, PTEX.
PGAdb-builder: A web service tool for creating pan-genome allele database for molecular fine typing
Authors: Liu Yen-Yi , Chiou Chien-Shun , Chen Chih-Chieh
Published: November 2016

With the advance of next generation sequencing techniques, whole genome sequencing (WGS) is expected to become the optimal method for molecular subtyping of bacterial isolates. To use WGS as a general subtyping method for disease outbreak investigation and surveillance, the layout of WGS-based typing must be comparable among laboratories. Whole genome multilocus sequence typing (wgMLST) is an approach that achieves this requirement. To apply wgMLST as a standard subtyping approach, a pan-genome allele database (PGAdb) for the population of a bacterial organism must first be established. We present a free web service tool, PGAdb-builder (http://wgmlstdb.imst.nsysu.edu.tw), for the construction of bacterial PGAdb. The effectiveness of PGAdb-builder was tested by constructing a pan-genome allele database for Salmonella enterica serovar Typhimurium, with the database being applied to create a wgMLST tree for a panel of epidemiologically well-characterized S. Typhimurium isolates. The performance of the wgMLST-based approach was as high as that of the SNP-based approach in Leekitcharoenphon’s study used for discerning among epidemiologically related and non-related isolates.
Design, synthesis and biological evaluation of hybrids of β-carboline and salicylic acid as potential anticancer and apoptosis inducing agents
Authors: Xu Qi-Bing , Chen Xiang-Fan , Feng Jiao , Miao Jie-Fei , Liu Ji , Liu Feng-Tao , Niu Bi-Xi , Cai Jin-Yang , Huang Chao , Zhang Yanan , Ling Yong
Published: November 2016

A novel series of hybrids (7a-l, 8a-l) from β-carboline and salicylic acid (SA) were designed and synthesized, and their in vitro biological activities were evaluated. Most of the hybrids displayed potent antiproliferative activity against five cancer cell lines in vitro, showing potencies superior to 5-FU and harmine. In particular, compound 8h selectively inhibited proliferation of liver cancer SMMC-7721 cells but not normal liver LO2 cells, and displayed greater inhibitory selectivity than intermediate 5h and SA. 8h also induced cancer cell apoptosis in an Annexin V-FITC/propidium iodide flow cytometry assay, and triggered the mitochondrial/caspase apoptosis by decreasing mitochondrial membrane potential which was associated with up-regulation of Bax, down-regulation of Bcl-2 and activation levels of the caspase cascade in a concentration-dependent manner. Our findings suggest that the β-carboline/SA hybrids may hold greater promise as therapeutic agents for the intervention of human cancers.