bluuebunny/crossref_metadata_2025 huggingface dataset:
State of the Art and Trends in the Monitoring, Detection and Diagnosis of Failures in Electric Induction Motors
Authors: Merizalde Yuri , Hernández-Callejo Luis , Duque-Perez Oscar
Published: July 2017

Despite the complex mathematical models and physical phenomena on which it is based, the simplicity of its construction, its affordability, the versatility of its applications and the relative ease of its control have made the electric induction motor an essential element in a considerable number of processes at the industrial and domestic levels, in which it converts electrical energy into mechanical energy. The importance of this type of machine for the continuity of operation, mainly in industry, is such that, in addition to being an important part of the study programs of careers related to this branch of electrical engineering, a large number of investigations into monitoring, detecting and quickly diagnosing its incipient faults due to a variety of factors have been conducted. This bibliographic research aims to analyze the conceptual aspects of the first discoveries that served as the basis for the invention of the induction motor, ranging from the development of the Fourier series, the Fourier transform mathematical formula in its different forms and the measurement, treatment and analysis of signals to techniques based on artificial intelligence and soft computing. This research also includes topics of interest such as fault types and their classification according to the engine, software and hardware parts used and modern approaches or maintenance strategies.
Analysis of Overlying Strata Movement and Behaviors in Caving and Solid Backfilling Mixed Coal Mining
Authors: Huang Yanli , Zhang Jixiong , Yin Wei , Sun Qiang
Published: July 2017

Based on techniques of close upper protective coal-rock layer mining, relieved gas extraction, and underground gangue washing-discharging-backfilling, this paper initiates the concept of mixed fully-mechanized coal mining, which combines a solid backfilling method and a caving method (hereinafter referred to as “backfill and caving mixed mining”). After the principle and key techniques are introduced, a physical simulation experiment and a numerical simulation are used to study the characteristics of the overlying strata’s fracture development, the main roof subsidence, the stress field and its influence area in the transition area with the length ratios of the backfilling section and the caving section, and the advancing distance of the mixed longwall face. Thus, the lengths of the caving section and the backfilling section, the parameters of the support system in the transition section, and the design process of the mixed longwall face are presented. In practice, the mixed longwall face Ji15-31010 in Ping-dingshan No. 12 Colliery proves that the designed lengths of 120 m and 100 m for the backfilling section and the caving section, respectively, are appropriate. The monitoring results of the hydraulic support working resistance show that the supports were working well in general; the maximum growth height of the overlying strata fracture is 18 m; the gas drainage efficiency is up to 80% and the average gas concentration is 0.1 g/m3; a large quantity of gangue generated in the Ji14 seam is disposed underground; coal and gas are extracted simultaneously; and significant environmental and economic benefits are realized.
A Novel Multi-Point Excitation Fatigue Testing Method for Wind Turbine Rotor Blades
Authors: Pan Zujin , Wu Jianzhong
Published: July 2017

Wind turbine blades have to withstand the rigorous test of 20–25 years of service. Fatigue testing is an accurate method used to verify blade reliability. Multi-point excitation could better fit the fatigue damage distribution, which reduces the power output of a single exciter and saves testing energy consumption. The amplitude, phase, and frequency characteristics of the fatigue test system and, moreover, the relationship between the excitation force, damping, and the amplitude variation of the blade, are analyzed by the Lagrangian equation and the finite element simulation method. The full-scale fatigue test of an equivalent full cycle life in the flapwise direction is carried out by multi-excitation. When the frequency and phase of the multi-point exciters are consistent, the maximum vibration effect can be exerted. When the phase difference of the dual exciters is 180°, the vibration effect produced by the dual exciters can be equivalent to each other. The blade amplitude is proportional to excitation forces, while inversely proportional to the damping ratio. The bending moment deviation of the blade is controlled within 9.2%; moreover, the energy consumption is 40% lower than that of the single-point excitation. The use of multi-point excitation allows loading the blade with high precision, stable operation, and low cost, which provides the theoretical and experimental basis for the fatigue test of large wind turbine blades.
Pole-to-Ground Fault Analysis and Fast Protection Scheme for HVDC Based on Overhead Transmission Lines
Authors: Xue Shimin , Lian Jie , Qi Jinlong , Fan Boyang
Published: July 2017

Flexible direct current (DC) transmission network technology is an effective method for large capacity clean energy access to power grids, but the DC short-circuit fault detection for it is a difficult problem. In this paper, the pole-to-ground fault transient characteristics in a multi-terminal DC power grid, based on overhead transmission lines and DC circuit breakers, are analyzed firstly. Then, a fast protection scheme is proposed according to the fault transient characteristics. Only local information is utilized for fault detection and location in the proposed scheme. Moreover, the scheme is verified to have the advantages of fast action speed, high reliability and the ability to resist the transition resistance. A four terminal DC power grid model based on actual engineering parameters is established in PSCAD/EMTDC, and the validity of the protection scheme under different fault conditions is verified by simulation results.
Epitranscriptome and FMRP Regulated mRNA Translation
Authors: Majumder Pritha , Chatterjee Biswanath , Shen C.-K.
Published: July 2017

An important regulatory mechanism affecting mRNA translation involves various covalent modifications of RNA, which establish distinct epitranscriptomic signatures that actively influence various physiological processes. Dendritic translation in mammalian neurons is a potent target for RNA modification-based regulation. In this mini-review, we focus on the effect of potential RNA modifications on the spatiotemporal regulation of the dendritic translation of mRNAs, which are targeted by two important neuronal translational co-regulators, namely TDP-43 and Fragile X Mental Retardation Protein (FMRP).
Barriers to the Adoption of Alley Cropping as a Climate-Smart Agriculture Practice: Lessons from Maize Cultivation among the Maya in Southern Belize
Authors: Kongsager Rico
Published: July 2017

Climate-smart agriculture (CSA) is proposed as a necessity, as the agricultural sector will need to adapt to resist future climatic change, to which high emissions from the sector contribute significantly. This study, which is an exploratory case study based on qualitative interviews and field observations, investigates the barriers to making a CSA-adjustment in maize production among Maya communities in southern Belize. The adjustment is alley cropping, which is a low-input adjustment that has the potential to result in both adaptation and mitigation benefits, and furthermore, to enhance food security. The findings show that a CSA-adjustment in small-scale maize production in Maya villages in southern Belize is possible in principle, though several barriers can make the overall climate-smart objective difficult to implement in practice. The barriers are of a proximate and indirect nature, exist at different spatial scales, and involve various levels of governance. The barriers are shown to be land tenure, market access, and changes in the traditional culture, however, these barriers are not homogenous across the villages in the region. To break down the barriers an overall district-level strategy is possible, but the toolbox should contain a wide variety of approaches. These could happen, for instance, through alterations to land tenure and the land taxation system nationally, enhancement of the agricultural extension system to ease access to knowledge and input at the district level, and support to a less complex governance structure at the village level.
Evolutionary Significance of Wolbachia-to-Animal Horizontal Gene Transfer: Female Sex Determination and the f Element in the Isopod Armadillidium vulgare
Authors: Cordaux Richard , Gilbert Clément
Published: July 2017

An increasing number of horizontal gene transfer (HGT) events from bacteria to animals have been reported in the past years, many of which involve Wolbachia bacterial endosymbionts and their invertebrate hosts. Most transferred Wolbachia genes are neutrally-evolving fossils embedded in host genomes. A remarkable case of Wolbachia HGT for which a clear evolutionary significance has been demonstrated is the “f element”, a nuclear Wolbachia insert involved in female sex determination in the terrestrial isopod Armadillidium vulgare. The f element represents an instance of bacteria-to-animal HGT that has occurred so recently that it was possible to infer the donor (feminizing Wolbachia closely related to the wVulC Wolbachia strain of A. vulgare) and the mechanism of integration (a nearly complete genome inserted by micro-homology-mediated recombination). In this review, we summarize our current knowledge of the f element and discuss arising perspectives regarding female sex determination, unstable inheritance, population dynamics and the molecular evolution of the f element. Overall, the f element unifies three major areas in evolutionary biology: symbiosis, HGT and sex determination. Its characterization highlights the tremendous impact sex ratio distorters can have on the evolution of sex determination mechanisms and sex chromosomes in animals and plants.
Boron Tolerance in Aspergillus nidulans Is Sustained by the SltA Pathway Through the SLC-Family Transporters SbtA and SbtB
Authors: Villarino María , Etxebeste Oier , Mendizabal Gorka , Garzia Aitor , Ugalde Unai , Espeso Eduardo A.
Published: July 2017

Microbial cells interact with the environment by adapting to external changes. Signal transduction pathways participate in both sensing and responding in the form of modification of gene expression patterns, enabling cell survival. The filamentous fungal-specific SltA pathway regulates tolerance to alkalinity, elevated cation concentrations and, as shown in this work, also stress conditions induced by borates. Growth of sltA− mutants is inhibited by increasing millimolar concentrations of boric acid or borax (sodium tetraborate). In an attempt to identify genes required for boron-stress response, we determined the boric acid or borax-dependent expression of sbtA and sbtB, orthologs of Saccharomyces cerevisiae bor1, and a reduction in their transcript levels in a ΔsltA mutant. Deletion of sbtA, but mainly that of sbtB, decreased the tolerance to boric acid or borax. In contrast, null mutants of genes coding for additional transporters of the Solute Carrier (SLC) family, sB, sbtD or sbtE, showed an unaltered growth pattern under the same stress conditions. Taken together, our results suggest that the SltA pathway induces, through SbtA and SbtB, the export of toxic concentrations of borates, which have largely recognized antimicrobial properties.
Land Use and Land Cover Change in the Qinghai Lake Region of the Tibetan Plateau and Its Impact on Ecosystem Services
Authors: Gong Jian , Li Jingye , Yang Jianxin , Li Shicheng , Tang Wenwu
Published: July 2017

Exploration of land use and land cover change (LULCC) and its impacts on ecosystem services in Tibetan plateau is valuable for landscape and environmental conservation. In this study, we conduct spatial analysis on empirical land use and land cover data in the Qinghai Lake region for 1990, 2000, and 2010 and simulate land cover patterns for 2020. We then evaluate the impacts of LULCC on ecosystem service value (ESV), and analyze the sensitivity of ESV to LULCC to identify the ecologically sensitive area. Our results indicate that, from 1990 to 2010, the area of forest and grassland increased while the area of unused land decreased. Simulation results suggest that the area of grassland and forest will continue to increase and the area of cropland and unused land will decrease for 2010–2020. The ESV in the study area increased from 694.50 billion Yuan in 1990 to 714.28 billion Yuan in 2000, and to 696.72 billion Yuan in 2020. Hydrology regulation and waste treatment are the top two ecosystem services in this region. The towns surrounding the Qinghai Lake have high ESVs, especially in the north of the Qinghai Lake. The towns with high ESV sensitivity to LULCC are located in the northwest, while the towns in the north of the Qinghai Lake experienced substantial increase in sensitivity index from 2000–2010 to 2010–2020, especially for three regulation services and aesthetic landscape provision services.
Electrochemical Oxidation of EDTA in Nuclear Wastewater Using Platinum Supported on Activated Carbon Fibers
Authors: Zhao Bo , Zhu Wenkun , Mu Tao , Hu Zuowen , Duan Tao
Published: July 2017

A novel Pt/ACF (Pt supported on activated carbon fibers) electrode was successfully prepared with impregnation and electrodeposition method. Characterization of the electrodes indicated that the Pt/ACF electrode had a larger effective area and more active sites. Electrochemical degradation of ethylenediaminetetra-acetic acid (EDTA) in aqueous solution with Pt/ACF electrodes was investigated. The results showed that the 3% Pt/ACF electrode had a better effect on EDTA removal. The operational parameters influencing the electrochemical degradation of EDTA with 3% Pt/ACF electrode were optimized and the optimal removal of EDTA and chemical oxygen demand (COD) were 94% and 60% after 100 min on condition of the electrolyte concentration, initial concentration of EDTA, current density and initial value of pH were 0.1 mol/L, 300 mg/L, 40 mA/cm2 and 5.0, respectively. The degradation intermediates of EDTA in electrochemical oxidation with 3% Pt/ACF electrode were identified by gas chromatography-mass spectrum (GC-MS).
Development and Validation of a Behavioural Index for Adaptation to High Summer Temperatures among Urban Dwellers
Authors: Valois Pierre , Talbot Denis , Caron Maxime , Carrier Marie-Pier , Morin Alexandre , Renaud Jean-Sébastien , Jacob Johann , Gosselin Pierre
Published: July 2017

One of the consequences of climate change is the growing number of extreme weather events, including heat waves, which have substantial impacts on the health of populations. From a public health standpoint, it is vital to ensure that people can adapt to high heat, particularly in cities where heat islands abound. Identifying indicators to include in a parsimonious index would help better differentiate individuals who adapt well to heat from those who do not adapt as well. This study aimed at developing and validating a summer heat adaptation index for residents of the 10 largest cities in the province of Québec, Canada. A sample of 2000 adults in 2015 and 1030 adults in 2016 completed a telephone questionnaire addressing their adoption (or non-adoption) of behaviours recommended by public health agencies to protect themselves during periods of high temperature, and their perceptions of how high summer heat affects their mental and physical health. Item analysis, confirmatory factor analysis, multiple correspondence analysis, measurement invariance analyses and criterion-validity analyses were used to develop a 12-behaviour heat adaptation index for distinguishing between individuals who adapt well to high temperatures and those who do not adapt as well. The results indicated that the measurement and the factor structure of the index were invariant (equivalent) across the two independent samples of participants who completed the questionnaire at different times one year apart, an important prerequisite for unambiguous interpretation of index scores across groups and over time. The results also showed that individuals who perceived more adverse effects on their physical or mental health adopted more preventive behaviours during periods of high temperatures and humidity conditions compared to those who felt lesser or no effects. This study thus presents support for the validity of the index that could be used in future studies to monitor preventive behaviours adoption during summer periods of high temperature.
Dissipation and Residues of Pyrethrins in Leaf Lettuce under Greenhouse and Open Field Conditions
Authors: Pan Lixiang , Feng Xiaoxiao , Zhang Hongyan
Published: July 2017

Pyrethrins are nowadays widely used for prevention and control of insects in leaf lettuce. However, there is a concern about the pesticide residue in leaf lettuce. A reliable analytical method for determination of pyrethrins (pyrethrin—and П, cinerin І and П, and jasmolin І and П) in leaf lettuce was developed by using gas chromatography–mass spectrometry (GC–MS). Recoveries of pyrethrins in leaf lettuce at three spiking levels were 99.4–104.0% with relative standard deviations of 0.9–3.1% (n = 5). Evaluation of dissipation and final residues of pyrethrins in leaf lettuce were determined at six different locations, including the open field, as well as under greenhouse conditions. The initial concentration of pyrethrins in greenhouse (0.57 mg/kg) was higher than in open field (0.25 mg/kg) and the half-life for pyrethrins disappearance in field lettuce (0.7 days) was less than that greenhouse lettuce (1.1 days). Factors such as rainfall, solar radiation, wind speed, and crop growth rate are likely to have caused these results. The final residue in leaf lettuce was far below the maximum residue limits (MRLs) (1 mg/kg established by the European Union (EU), Australia, Korea, Japan).
Towards Enhancing Integrated Pest Management Based on Volunteered Geographic Information
Authors: Yan Yingwei , Feng Chen-Chieh , Chang Klarissa
Published: July 2017

Integrated pest management (IPM) involves integrating multiple pest control methods based on site information obtained through inspection, monitoring, and reports. IPM has been deployed to achieve the judicious use of pesticides and has become one of the most important methods of securing agricultural productivity. Despite the efforts made to strengthen IPM during the past decades, overuse as well as indiscriminate use of pesticides is still common. This problem is particularly serious in underserved farming communities which suffer from ineffectiveness with respect to pest management information collection and dissemination. The recent development of volunteered geographic information (VGI) offers an opportunity to the general public to create and receive ubiquitous, cost-effective, and timely geospatial information. Therefore, this study proposes to enhance IPM through establishing a VGI-based IPM. As a starting point of this line of research, this study explored how such geospatial information can contribute to IPM enhancement. Based on this, a conceptual framework of VGI interaction was built to guide the establishment of VGI-based IPM. To implement VGI-based IPM, a mobile phone platform was developed. In addition, a case study was conducted in the town of Shuibian in Jiangxi province of China to demonstrate the effectiveness of the proposed approach. In the case study, by analyzing infestation incidents of an overwintering outbreak of striped rice stem borers voluntarily reported by farmers through mobile phones, spatiotemporal infestation patterns of the borers throughout the study area were revealed and disseminated to the farmers. These patterns include the dates and degree-days the pest infestations intensified, and the orientation or spatial structural variations of the clustering of the infestations. This case study showcased the unique merit of VGI in enhancing IPM, namely the acquisition of previously unrecorded spatial data in a cost-effective and real-time manner for discovering and disseminating previously unknown pest management knowledge.
FOSS Tools and Applications for Education in Geospatial Sciences
Authors: Ciolli Marco , Federici Bianca , Ferrando Ilaria , Marzocchi Roberto , Sguerso Domenico , Tattoni Clara , Vitti Alfonso , Zatelli Paolo
Published: July 2017

While the theory and implementation of geographic information systems (GIS) have a history of more than 50 years, the development of dedicated educational tools and applications in this field is more recent. This paper presents a free and open source software (FOSS) approach for education in the geospatial disciplines, which has been used over the last 20 years at two Italian universities. The motivations behind the choice of FOSS are discussed with respect to software availability and development, as well as educational material licensing. Following this philosophy, a wide range of educational tools have been developed, covering topics from numerical cartography and GIS principles to the specifics regarding different systems for the management and analysis of spatial data. Various courses have been implemented for diverse recipients, ranging from professional training workshops to PhD courses. Feedback from the students of those courses provides an invaluable assessment of the effectiveness of the approach, supplying at the same time directions for further improvement. Finally, lessons learned after 20 years are discussed, highlighting how the management of educational materials can be difficult even with a very open approach to licensing. Overall, the use of free and open source software for geospatial (FOSS4G) science provides a clear advantage over other approaches, not only simplifying software and data management, but also ensuring that all of the information related to system design and implementation is available.
Combination Therapy of PEG-HM-3 and Methotrexate Retards Adjuvant-Induced Arthritis
Authors: Hao Jingchao , Wu Xiaodong , Setrerrahmane Sarra , Qian Kun , Hou Yueying , Yu Liting , Lin Chenyu , Wu Qianqian , Xu Hanmei
Published: July 2017

At present, the early phenomenon of inflammatory angiogenesis is rarely studied in Rheumatoid arthritis (RA). Previous research found that PEG-HM-3, an integrin inhibitor, possessed anti-angiogenesis and anti-rheumatic activity. In this study, the advantages of inhibiting angiogenesis and immune cell adhesion and migration, as well as the benefits of anti-arthritis effects, were evaluated using a combination of PEG-HM-3 and methotrexate (MTX). In vitro, spleen cell proliferation and the levels of tumor necrosis factor α (TNF-α) in macrophage supernatant were assessed. Hind paw edema, arthritis index, clinical score, body weight and immunohistochemistry (IHC) of the spleen, thymus, and joint cavity were evaluated in vivo in adjuvant-induced arthritis rats. Joints of the left hind paws were imaged by X-ray. The expression of the toll-like receptor 4 (TLR-4) protein was assessed in lipopolysaccharide (LPS)-induced synoviocytes. PEG-HM-3 combined with MTX significantly reduced primary and secondary swelling of the hind paws, the arthritis index, the clinical score and bone erosion. The results of IHC showed that the levels of interleukin-6 (IL-6) in spleens and the levels of TNF-α, CD31 (cluster of differentiation 31), and CD105 in the joint cavity were decreased. The body weight of rats was maintained during combination therapy. Ankle cavity integrity, and bone erosion and deformity were improved in combination treatment. The expression of TLR-4 was significantly reduced with combination treatment in rat synoviocytes. Co-suppression of both inflammation and angiogenesis in arthritis was achieved in this design with combination therapy. The activity of nuclear transcription factor (NF-κB) and the expression of inflammatory factors were down regulated via integrin αvβ3 and TLR-4 signaling pathways. In the future, the application of this combination can be a candidate in early and mid-term RA therapy.
Dietary Modulation of Oxidative Stress in Alzheimer’s Disease
Authors: Thapa Arjun , Carroll Nick
Published: July 2017

Cells generate unpaired electrons, typically via oxygen- or nitrogen-based by-products during normal cellular respiration and under stressed situations. These pro-oxidant molecules are highly unstable and may oxidize surrounding cellular macromolecules. Under normal conditions, the reactive oxygen or nitrogen species can be beneficial to cell survival and function by destroying and degrading pathogens or antigens. However, excessive generation and accumulation of the reactive pro-oxidant species over time can damage proteins, lipids, carbohydrates, and nucleic acids. Over time, this oxidative stress can contribute to a range of aging-related degenerative diseases such as cancer, diabetes, macular degeneration, and Alzheimer’s, and Parkinson’s diseases. It is well accepted that natural compounds, including vitamins A, C, and E, β-carotene, and minerals found in fruits and vegetables are powerful anti-oxidants that offer health benefits against several different oxidative stress induced degenerative diseases, including Alzheimer’s disease (AD). There is increasing interest in developing anti-oxidative therapeutics to prevent AD. There are contradictory and inconsistent reports on the possible benefits of anti-oxidative supplements; however, fruits and vegetables enriched with multiple anti-oxidants (e.g., flavonoids and polyphenols) and minerals may be highly effective in attenuating the harmful effects of oxidative stress. As the physiological activation of either protective or destructive pro-oxidant behavior remains relatively unclear, it is not straightforward to relate the efficacy of dietary anti-oxidants in disease prevention. Here, we review oxidative stress mediated toxicity associated with AD and highlight the modulatory roles of natural dietary anti-oxidants in preventing AD.
The Role of Tumor Microenvironment in Chemoresistance: To Survive, Keep Your Enemies Closer
Authors: Senthebane Dimakatso Alice , Rowe Arielle , Thomford Nicholas Ekow , Shipanga Hendrina , Munro Daniella , Mazeedi Mohammad A. M. Al , Almazyadi Hashim A. M. , Kallmeyer Karlien , Dandara Collet , Pepper Michael S. , Parker M. Iqbal , Dzobo Kevin
Published: July 2017

Chemoresistance is a leading cause of morbidity and mortality in cancer and it continues to be a challenge in cancer treatment. Chemoresistance is influenced by genetic and epigenetic alterations which affect drug uptake, metabolism and export of drugs at the cellular levels. While most research has focused on tumor cell autonomous mechanisms of chemoresistance, the tumor microenvironment has emerged as a key player in the development of chemoresistance and in malignant progression, thereby influencing the development of novel therapies in clinical oncology. It is not surprising that the study of the tumor microenvironment is now considered to be as important as the study of tumor cells. Recent advances in technological and analytical methods, especially ‘omics’ technologies, has made it possible to identify specific targets in tumor cells and within the tumor microenvironment to eradicate cancer. Tumors need constant support from previously ‘unsupportive’ microenvironments. Novel therapeutic strategies that inhibit such microenvironmental support to tumor cells would reduce chemoresistance and tumor relapse. Such strategies can target stromal cells, proteins released by stromal cells and non-cellular components such as the extracellular matrix (ECM) within the tumor microenvironment. Novel in vitro tumor biology models that recapitulate the in vivo tumor microenvironment such as multicellular tumor spheroids, biomimetic scaffolds and tumor organoids are being developed and are increasing our understanding of cancer cell-microenvironment interactions. This review offers an analysis of recent developments on the role of the tumor microenvironment in the development of chemoresistance and the strategies to overcome microenvironment-mediated chemoresistance. We propose a systematic analysis of the relationship between tumor cells and their respective tumor microenvironments and our data show that, to survive, cancer cells interact closely with tumor microenvironment components such as mesenchymal stem cells and the extracellular matrix.
In Situ β-Glucan Fortification of Cereal-Based Matrices by Pediococcus parvulus 2.6: Technological Aspects and Prebiotic Potential
Authors: Pérez-Ramos Adrián , Mohedano María Luz , López Paloma , Spano Giuseppe , Fiocco Daniela , Russo Pasquale , Capozzi Vittorio
Published: July 2017

Bacterial exopolysaccharides produced by lactic acid bacteria are of increasing interest in the food industry, since they might enhance the technological and functional properties of some edible matrices. In this work, Pediococcus parvulus 2.6, which produces an O2-substituted (1,3)-β-d-glucan exopolysaccharide only synthesised by bacteria, was proposed as a starter culture for the production of three cereal-based fermented foods. The obtained fermented matrices were naturally bio-fortified in microbial β-glucans, and used to investigate the prebiotic potential of the bacterial exopolysaccharide by analysing the impact on the survival of a probiotic Lactobacillus plantarum strain under starvation and gastrointestinal simulated conditions. All of the assays were performed by using as control of the P. parvulus 2.6’s performance, the isogenic β-glucan non-producing 2.6NR strain. Our results showed a differential capability of P. parvulus to ferment the cereal flours. During the fermentation step, the β-glucans produced were specifically quantified and their concentration correlated with an increased viscosity of the products. The survival of the model probiotic L. plantarum WCFS1 was improved by the presence of the bacterial β-glucans in oat and rice fermented foods under starvation conditions. The probiotic bacteria showed a significantly higher viability when submitted to a simulated intestinal stress in the oat matrix fermented by the 2.6 strain. Therefore, the cereal flours were a suitable substrate for in situ bio-fortification with the bacterial β-glucan, and these matrices could be used as carriers to enhance the beneficial properties of probiotic bacteria.
Aquatic Insects and their Potential to Contribute to the Diet of the Globally Expanding Human Population
Authors: Williams D. , Williams Siân
Published: July 2017

Of the 30 extant orders of true insect, 12 are considered to be aquatic, or semiaquatic, in either some or all of their life stages. Out of these, six orders contain species engaged in entomophagy, but very few are being harvested effectively, leading to over-exploitation and local extinction. Examples of existing practices are given, ranging from the extremes of including insects (e.g., dipterans) in the dietary cores of many indigenous peoples to consumption of selected insects, by a wealthy few, as novelty food (e.g., caddisflies). The comparative nutritional worth of aquatic insects to the human diet and to domestic animal feed is examined. Questions are raised as to whether natural populations of aquatic insects can yield sufficient biomass to be of practicable and sustained use, whether some species can be brought into high-yield cultivation, and what are the requirements and limitations involved in achieving this?
Use of Multibeam and Dual-Beam Sonar Systems to Observe Cavitating Flow Produced by Ferryboats: In a Marine Renewable Energy Perspective
Authors: Francisco Francisco , Carpman Nicole , Dolguntseva Irina , Sundberg Jan
Published: July 2017

With the prospect to deploy hydrokinetic energy converters in areas with heavy boat traffic, a study was conducted to observe and assess the depth range of cavitating flow produced by ferryboats in narrow channels. This study was conducted in the vicinity of Finnhamn Island in Stockholm Archipelago. The objectives of the survey were to assess whether the sonar systems were able to observe and measure the depth of what can be cavitating flow (in a form of convected cloud cavitation) produced by one specific type of ferryboats frequently operating in that route, as well as investigate if the cavitating flow within the wake would propagate deep enough to disturb the water column underneath the surface. A multibeam and a dual-beam sonar systems were used as measurement instruments. The hypothesis was that strong and deep wake can disturb the optimal operation of a hydrokinetic energy converter, therefore causing damages to its rotors and hydrofoils. The results showed that both sonar system could detect cavitating flows including its strength, part of the geometrical shape and propagation depth. Moreover, the boat with a propeller thruster produced cavitating flow with an intense core reaching 4 m of depth while lasting approximately 90 s. The ferry with waterjet thruster produced a less intense cavitating flow; the core reached depths of approximately 6 m, and lasted about 90 s. From this study, it was concluded that multibeam and dual-beam sonar systems with operating frequencies higher than 200 kHz were able to detect cavitating flows in real conditions, as long as they are properly deployed and the data properly analyzed.
Improvement of Cr-Co-Mo Membrane Surface Used as Barrier for Bone Regeneration through UV Photofunctionalization: An In Vitro Study
Authors: Decco Oscar , Zuchuat Jésica , Farkas Nicolás
Published: July 2017

Although there are several studies of the ultraviolet (UV) light-mediated photofunctionalization of titanium for use as implant material, the underlying mechanism is not fully understood. However, the results of in vitro and in vivo studies are very encouraging. The use of UV photofunctionalization as a surface treatment on other implant materials, as the Cr-Co-Mo alloy, has not been explored in depth. Using sandblasted Cr-Co-Mo discs, the surface photofunctionalization was studied for ultraviolet A (UVA, 365 nm) and ultraviolet C (UVC, 254 nm), and the surfaces were evaluated for their ability to sustain hydroxyapatite crystal growth through incubation in simulated body fluid for a seven-day period. The variation of the pre- and post-irradiation contact angle and surface composition was determined through the quantification of the weight percentage of Ca and P crystals by the EDAX ZAF method (EDS). Statistically significant differences (p < 0.05) were found for samples irradiated with UVA over 48 h, corresponding with hydrophilic surfaces, and the same result was found for samples exposed to 3 h of UVC. Superhydrophilic surfaces were found in samples irradiated for 12, 24 and 48 h with UVC. The decrease in the carbon content is related with the increase in the surface content of Ca and P, and vice versa over the Cr-Co-Mo surfaces.
Influences of Thermal Treatment on the Dielectric Performances of Polystyrene Composites Reinforced by Graphene Nanoplatelets
Authors: Fan Benhui , Liu Yu , He Delong , Bai Jinbo
Published: July 2017

Dielectric properties of composites near percolation threshold (fc) are often sensitive to thermal treatments, and the annealing temperature is usually associated with a polymer’s rheological properties. In this study, the influences of the thermal treatment on dielectric properties are investigated for the polystyrene (PS) matrix composite reinforced by graphene nanoplatelets (GNP) fillers near fc. It can be found that the thermal treatment can not only increase the dielectric constant, but also decrease the dielectric loss for the PS/GNP composite. This interesting phenomenon possibly happens in the interfacial region of PS/GNP with the thickness about 4–6 nm according to the electron energy-loss spectroscopy (EELS) results. The free volumes around the interface can be easily altered by the movement of polymeric segments after annealing at the glass transition temperature.
The Microstructural Characterization of Multiferroic LaFeO3-YMnO3 Multilayers Grown on (001)- and (111)-SrTiO3 Substrates by Transmission Electron Microscopy
Authors: Pang Bin , Sun Lei , Shen Xuan , Lv Yang-Yang , Li Xiao , Wu Fei-Xiang , Yao Shu-Hua , Zhou Jian , Zhang Shan-Tao , Chen Y.B.
Published: July 2017

The microstructure of multiferroic LaFeO3-YMnO3 (LFO-YMO) multilayers grown on (001)- and (111)-SrTiO3 substrates is characterized by the transmission electron microscopy (TEM). Detailed TEM characterization reveals that LFO-YMO multilayers grown on both substrates have clear layer-by-layer morphology and distinct chemical-composition layered structure. The most notable feature is that LFO-YMO multilayers grown on (001)-SrTiO3 substrate have three types of domains, while those on (111)-SrTiO3 have only one. The multi-/twin- domain structure can be qualitatively explained by the lattice mismatch in this system. The details of the domain structure of LFO-YMO multilayers are crucial to understanding their magnetic properties.
Interaction of Shear and Rayleigh–Lamb Waves with Notches and Voids in Plate Waveguides
Authors: Pau Annamaria , Achillopoulou Dimitra
Published: July 2017

This paper investigates the interaction of different shear- and Rayleigh–Lamb-guided waves in plates with a discontinuity such as a notch or an internal void. The problem was solved numerically using a finite element model and by exploiting an analytical solution obtainable for the double sharp changes of the cross-section that served as a reference. We aimed to elucidate the relation between the size and shape of the discontinuity and the reflection and transmission coefficients of the scattered field. Different sizes and profiles of the discontinuity were considered, with the shapes ranging from step changes of the height to ellipses, both symmetric and nonsymmetric. Regimes related to low and high values of the product frequency multiplied by the height of the plate were investigated. These showed how the mode conversion was related to the symmetry between the incident mode and the discontinuity, and to the actual existence of multiple propagating modes. The analysis presented was motivated by the need to set up procedures that exploit propagating waves not only to detect the presence of a notch, but also to characterize its size and shape.
Variable Shape Parameter Strategy in Local Radial Basis Functions Collocation Method for Solving the 2D Nonlinear Coupled Burgers’ Equations
Authors: Nojavan Hananeh , Abbasbandy Saeid , Allahviranloo Tofigh
Published: July 2017

This study aimed at investigating a local radial basis function collocation method (LRBFCM) in the reproducing kernel Hilbert space. This method was, in fact, a meshless one which applied the local sub-clusters of domain nodes for the approximation of the arbitrary field. For time-dependent partial differential equations (PDEs), it would be changed to a system of ordinary differential equations (ODEs). Here, we intended to decrease the error through utilizing variable shape parameter (VSP) strategies. This method was an appropriate way to solve the two-dimensional nonlinear coupled Burgers’ equations comprised of Dirichlet and mixed boundary conditions. Numerical examples indicated that the variable shape parameter strategies were more efficient than constant ones for various values of the Reynolds number.
Rapid Nucleic Acid Extraction and Purification Using a Miniature Ultrasonic Technique
Authors: Branch Darren , Vreeland Erika , McClain Jamie , Murton Jaclyn , James Conrad , Achyuthan Komandoor
Published: July 2017

Miniature ultrasonic lysis for biological sample preparation is a promising technique for efficient and rapid extraction of nucleic acids and proteins from a wide variety of biological sources. Acoustic methods achieve rapid, unbiased, and efficacious disruption of cellular membranes while avoiding the use of harsh chemicals and enzymes, which interfere with detection assays. In this work, a miniature acoustic nucleic acid extraction system is presented. Using a miniature bulk acoustic wave (BAW) transducer array based on 36° Y-cut lithium niobate, acoustic waves were coupled into disposable laminate-based microfluidic cartridges. To verify the lysing effectiveness, the amount of liberated ATP and the cell viability were measured and compared to untreated samples. The relationship between input power, energy dose, flow-rate, and lysing efficiency were determined. DNA was purified on-chip using three approaches implemented in the cartridges: a silica-based sol-gel silica-bead filled microchannel, nucleic acid binding magnetic beads, and Nafion-coated electrodes. Using E. coli, the lysing dose defined as ATP released per joule was 2.2× greater, releasing 6.1× more ATP for the miniature BAW array compared to a bench-top acoustic lysis system. An electric field-based nucleic acid purification approach using Nafion films yielded an extraction efficiency of 69.2% in 10 min for 50 µL samples.
Effects of Chlorhexidine-Encapsulated Mesoporous Silica Nanoparticles on the Anti-Biofilm and Mechanical Properties of Glass Ionomer Cement
Authors: Yan Huiyi , Yang Hongye , Li Kang , Yu Jian , Huang Cui
Published: July 2017

One of the primary causes for the failure of glass ionomer cement (GIC) is secondary caries. To enhance the anti-microbial performance of GIC without affecting its mechanical properties, chlorhexidine (CHX) was encapsulated in expanded-pore mesoporous silica nanoparticles (pMSN) to synthesize CHX@pMSN. CHX@pMSN was added at three mass fractions (1%, 5%, and 10% (w/w)) to GIC powder as the experimental groups. Pure GIC was set as the control group. The mechanical and anti-biofilm properties of GIC from each group were tested. The results demonstrated that CHX was successfully encapsulated on/into pMSN, and the encapsulating efficiency of CHX was 44.62% in CHX@pMSN. The anti-biofilm ability was significantly enhanced in all experimental groups (p < 0.001) compared with that in the control group. CHX was continuously released, and anti-biofilm ability was maintained up to 30 days. In addition, the mechanical properties (compressive strength, surface hardness, elastic modulus, water sorption, and solubility) of 1% (w/w) group were maintained compared with those in the control group (p > 0.05). In conclusion, adding 1% (w/w) CHX@pMSN to GIC led to conspicuous anti-biofilm ability and had no adverse effect on the mechanical properties of this restorative material. This study proposes a new strategy for preventing secondary caries by using CHX@pMSN-modified GIC.
Growth Mechanism Studies of ZnO Nanowires: Experimental Observations and Short-Circuit Diffusion Analysis
Authors: Shih Po-Hsun , Wu Sheng
Published: July 2017

Plenty of studies have been performed to probe the diverse properties of ZnO nanowires, but only a few have focused on the physical properties of a single nanowire since analyzing the growth mechanism along a single nanowire is difficult. In this study, a single ZnO nanowire was synthesized using a Ti-assisted chemical vapor deposition (CVD) method to avoid the appearance of catalytic contamination. Two-dimensional energy dispersive spectroscopy (EDS) mapping with a diffusion model was used to obtain the diffusion length and the activation energy ratio. The ratio value is close to 0.3, revealing that the growth of ZnO nanowires was attributed to the short-circuit diffusion.
Intra-Amniotic Administration (Gallus gallus) of Cicer arietinum and Lens culinaris Prebiotics Extracts and Duck Egg White Peptides Affects Calcium Status and Intestinal Functionality
Authors: Hou Tao , Kolba Nikolai , Glahn Raymond , Tako Elad
Published: July 2017

Calcium (Ca) is one of the most abundant inorganic elements in the human body and has many important physiological roles. Prebiotics and bioactive peptides are two important substances used to promote calcium uptake. However, the difference in mechanisms of the calcium uptake from these two supplements is not clear. By using the Gallus gallus model and the intra-amniotic administration procedure, the aim of this study was to investigate whether Ca status, intestinal functionality, and health-promoting bacterial populations were affected by prebiotics extracted from chickpea and lentil, and duck egg white peptides (DPs). Eleven groups (non-injected; 18 MΩ H2O; 4 mmol/L CaCl2; 50 mg/mL chickpea + 4 mmol/L CaCl2; 50 mg/mL lentil + 4 mmol/L CaCl2; 40 mg/mL DPs + 4 mmol/L CaCl2; 5 mg/mL Val-Ser-Glu-Glu (VSEE) + 4 mmol/L CaCl2; 50 mg/mL chickpea; 50 mg/mL lentil; 40 mg/mL DPs; 5 mg/mL VSEE) were utilized. Upon hatch, blood, cecum, small intestine, liver and bone were collected for assessment of serum bone alkaline phosphate level (BALP), the relative abundance of intestinal microflora, expression of Ca-related genes, brush border membrane (BBM) functional genes, and liver and bone mineral levels, respectively. The BALP level increased in the presence of lentil, DPs and VSEE (p < 0.05). The relative abundance of probiotics increased significantly (p < 0.05) by VSEE + Ca and chickpea. The expression of CalbindinD9k (Ca transporter) increased (p < 0.05) in Ca, chickpea + Ca and lentil + Ca groups. In addition, the brush border membrane functionality genes expressions increased (p < 0.05) by the chickpea or lentil extracts. Prebiotics and DPs beneficially affected the intestinal microflora and duodenal villus surface area. This research expands the understanding of the prebiotics’ properties of chickpea and lentil extracts, and peptides’ effects on calcium metabolism and gut health.
Iron Bioavailability Studies of the First Generation of Iron-Biofortified Beans Released in Rwanda
Authors: Glahn Raymond , Tako Elad , Hart Jonathan , Haas Jere , Lung’aho Mercy , Beebe Steve
Published: July 2017

This paper represents a series of in vitro iron (Fe) bioavailability experiments, Fe content analysis and polyphenolic profile of the first generation of Fe biofortified beans (Phaseolus vulgaris) selected for human trials in Rwanda and released to farmers of that region. The objective of the present study was to demonstrate how the Caco-2 cell bioassay for Fe bioavailability can be utilized to assess the nutritional quality of Fe in such varieties and how they may interact with diets and meal plans of experimental studies. Furthermore, experiments were also conducted to directly compare this in vitro approach with specific human absorption studies of these Fe biofortified beans. The results show that other foods consumed with beans, such as rice, can negatively affect Fe bioavailability whereas potato may enhance the Fe absorption when consumed with beans. The results also suggest that the extrinsic labelling approach to measuring human Fe absorption can be flawed and thus provide misleading information. Overall, the results provide evidence that the Caco-2 cell bioassay represents an effective approach to evaluate the nutritional quality of Fe-biofortified beans, both separate from and within a targeted diet or meal plan.