bluuebunny/crossref_metadata_2025 huggingface dataset:
Politics and Identity in the Argentine Army
Authors: Waisbord Silvio
Published: October 2022

Scholars and journalists have devoted considerable attention to analyzing the three military rebellions that rocked Argentine democracy in 1987 and 1988 (Boron 1987; Fontana 1988; López 1988; Stepan 1988). In addition to considering whether these revolts threatened the stability of the new political regime, academic and political interpretations have pointed to another issue stemming from the revolts: the emergence of a new generation of army officers with political goals and ideological values that differ from those prevailing in the upper levels of the Argentine military hierarchy. According to some observers, the experiences of middle-ranking officers during the last authoritarian regime produced a breach within the army that led, in the extreme view, to “two opposing armies.” This argument asserts that the Argentine Army currently appears divided between the high command (“oficiales superiores” made up of colonels and generals) and middle-ranking officers, who encompass “subalternos” (lieutenants and captains) and “jefes” (majors and lieutenant colonels). The split seems to have stemmed from differing political goals and ideological affiliations. The question, however, has remained speculative rather than being subjected to analytical research.
Bringing the Cuban Economy into Focus: Conceptual and Empirical Challenges
Authors: Pérez-López Jorge F.
Published: October 2022

Two differing views of the economy of contemporary Cuba continue to coexist. One portrays it as dynamic, largely unaffected by inflation, providing full employment for its citizens, diversified, and the unquestioned leader within Latin America in real economic growth in the 1970s and 1980s. The logical conclusion to be drawn from this view is that the Cuban economic model and its implementation in the island have been a success and that other developing countries in Latin America and elsewhere would do well to emulate the Cuban paradigm.
The Fertility of Maya and Ladino Women
Authors: Holian John
Published: October 2022

Second only to the Quechua-speaking peoples of the Andes, the Maya of southeastern Mexico and Guatemala constitute the “most impressive surviving American culture in the Western Hemisphere” (Vogt 1969a, 21). In Mexico the main division within the Maya falls between the highland population living in the state of Chiapas and the lowland group residing in the Yucatán Peninsula (Vogt 1969b). People of mixed Spanish and Indian ancestry, known locally as ladinos, make up most of the remaining population. Inspired by the well-known series of investigations of Indian and mestizo fertility in the Andean region, the present study seeks to describe within Mexico the fertility differences between the highland and lowland Maya and their ladino neighbors and, within the limits of the data, to account for the observed differentials.
Latin American Studies in the People's Republic of China: Current and Future Prospects
Authors: Zhang Sen-gen , Wang Ning-kun
Published: October 2022

Relations between China and Latin America date back hundreds of years and have intensified since the founding of the People's Republic of China. Recognizing that meaningful relations with Latin America require an understanding of that varied region, China has established appropriate study and research programs. This essay on current Latin American programs in the People's Republic of China will report on research organizations, research interests of Chinese scholars, and current trends within Latin American studies. The double objectives are to describe China's interest in Latin America and to establish closer contacts between Latin Americanists in China and the United States.
Fiction as History: The Case of João Ubaldo Ribeiro
Authors: Valente Luiz Fernando
Published: October 2022

Hayden White suggests inThe Content of the Formthat historiography depends upon the existence of a social center that allows the historian to locate events in relation to one another and “to charge them with ethical or moral significance” (White 1987, 11). This center makes it possible for the historical narrative—as opposed to the annals and the chronicle—to achieve closure. White specifies, however, that “in order to qualify as historical, an event must be susceptible to at least two narrations of its occurrence” (White 1987, 20). According to this perspective, the historical narrative is informed by the historian's need to assert his or her authority over other competing accounts of the past. In questioning the privileged position traditionally held by historians and suggesting that historians' discourse is only one of many paths leading to a truthful (re)presentation of the past, White's version of historiography holds particular appeal for those of us who, despite having been trained in fields other than history, consider ourselves to be authoritative interpreters of Brazil.
Economic Pressure Groups and Policy-Making in Venezuela: The Case of FEDECAMARAS Reconsidered
Authors: Salgado René
Published: October 2022

This article will focus on the interactions between the Venezuelan government and FEDECAMARAS, the umbrella organization of most Venezuelan private-sector groups, while a strategic policy was being formulated and implemented that FEDECAMARAS regarded as seriously detrimental to the interests of the Venezuelan private sector. This policy was developed after the official devaluation of the Venezuelan bolívar on 18 February 1983 and consisted of two stages: first, the government refused to supply foreign currency at the rate of 4.3 bolívares to the dollar, the predevaluation rate (PDR), for any foreign debts contracted by private enterprises prior to 18 February, a decision that forced many debtor companies to obtain foreign currency at the floating rate. This rate increased from approximately 8 bolívares to the dollar in March 1983 to some 25 to the dollar in late 1986. Second, the government imposed a price freeze and then price controls. This policy, which was simultaneously regulatory and redistributive, was vehemently opposed by FEDECAMARAS. Yet the results of the organization's efforts indicate that its actual influence has been overstated.
Rethinking Neocolonial Esthetics: Literature, Politics, and Intellectual Community in Cuba's Revista de Avance
Authors: Masiello Francine
Published: October 2022

Writers from Jorge Luis Borges to Alejo Carpentier have celebrated the role of literary journalism in Latin American cultural life. The periodical press mediates between author and public, between the heavy sea of tradition and the rising tide of the new, between the institutions that sustain convention and the spontaneous, vibrant eruptions that give life to the avant-garde. Literary journalism thus traces the struggles of writers against the canon while revealing their engagement in the political and aesthetic events of the day. But as one might expect, literary journalism also unravels the neat boundaries of the finished work or the book in the ongoing dialogue with contemporary publications and multifaceted speculations on culture. In this way, the pastiche of materials found in the modern review exposes the vivid heterogeneity of the intellectual field.
The phonetic realization of the plain uvular /q/ in a variety of South Bolivian Quechua
Authors: Gallagher Gillian
Published: October 2022

This paper presents an acoustic description of the production of the plain uvular /q/ in the speech of eight speakers of South Bolivian Quechua. While this sound patterns phonologically as a stop, its primary realization is as a voiced continuant. Variation is documented with respect to segmental and prosodic position. Segmentally, a voiced continuant is the most common realization intervocalically and after a rhotic, while a voiceless continuant is comparatively more frequent after a voiceless sibilant, and voiced stops are most common after a nasal. In post-pausal position, voiced continuant productions are still attested and are particularly common for certain speakers, suggesting that this sound category has been reanalyzed as a continuant. For other speakers, voiceless stop productions are common or preferred in post-pausal position, reflecting a standard prosodically conditioned lenition pattern. Interestingly, voiced stops also show increased frequency in post-pausal position. The production of the plain uvular is analyzed in spontaneous speech collected in an interview format, as well as in scripted speech from a word list task. A second analysis compares the realization of /q/ to the other three stops /p t k/ in the language in spontaneous speech, and finds significantly more continuant productions for /q/.
Protection against cystic echinococcosis in sheep using an Escherichia coli-expressed recombinant antigen (EG95) as a bacterin
Authors: Gauci Charles G. , Jenkins David J. , Lightowlers Marshall W.
Published: October 2022

The EG95 recombinant vaccine is protective against cystic echinococcus in animal intermediate hosts. Preparation of the existing, registered EG95 vaccines involves semi-purification of the vaccine protein, adding to the cost of production. Truncation of the EG95 cDNA, shortening both the amino and carboxy-termini of the protein, leads to high levels of recombinant protein expression. The recombinant EG95 protein was prepared as a bacterin from clarified, whole bacterial lysate, and used in a vaccine trial in sheep against an experimental challenge infection with Echinococcus granulosus eggs. The EG95 bacterin was found to induce 98% protection. Use of this in a new generation EG95 vaccine would simplify production, facilitate new sources of the vaccine and potentially enhance uptake of vaccination in control of E. granulosus transmission.
Host life-history traits predict haemosporidian parasite prevalence in tanagers (Aves: Thraupidae)
Authors: Aguiar de Souza Penha Victor , Maia Chaves Bicalho Domingos Fabricius , Fecchio Alan , Bell Jeffrey A. , Weckstein Jason D. , Ricklefs Robert E. , Braga Erika Martins , de Abreu Moreira Patrícia , Soares Letícia , Latta Steven , Tolesano-Pascoli Graziela , Alquezar Renata Duarte , Del-Claro Kleber , Manica Lilian Tonelli
Published: October 2022

Vector-borne parasites are important ecological drivers influencing life-history evolution in birds by increasing host mortality or susceptibility to new diseases. Therefore, understanding why vulnerability to infection varies within a host clade is a crucial task for conservation biology and for understanding macroecological life-history patterns. Here, we studied the relationship of avian life-history traits and climate on the prevalence ofPlasmodiumandParahaemoproteusparasites. We sampled 3569 individual birds belonging to 53 species of the family Thraupidae. Individuals were captured from 2007 to 2018 at 92 locations. We created 2 phylogenetic generalized least-squares models withPlasmodiumandParahaemoproteusprevalence as our response variables, and with the following predictor variables: climate PC1, climate PC2, body size, mixed-species flock participation, incubation period, migration, nest height, foraging height, forest cover, and diet. We found thatParahaemoproteusandPlasmodiumprevalence was higher in species inhabiting open habitats. Tanager species with longer incubation periods had higherParahaemoproteusprevalence as well, and we hypothesize that these longer incubation periods overlap with maximum vector abundances, resulting in a higher probability of infection among adult hosts during their incubation period and among chicks. Lastly, we found thatPlasmodiumprevalence was higher in species without migratory behaviour, with mixed-species flock participation, and with an omnivorous or animal-derived diet. We discuss the consequences of higher infection prevalence in relation to life-history traits in tanagers.
The influence of burial depth on germination and establishment of seeds in chimpanzee faeces, Taï National Park, Côte d’Ivoire
Authors: Koffi Kouadio Kan S. , Dosso Kanvaly , Aristophanous Marios , Moretto Philippe , Tiho Seydou , Wittig Roman M.
Published: October 2022

One of the many ecosystem services provided by dung beetles is that of secondary seed dispersal. This paper experimentally evaluates the effectiveness of this service using the chimpanzee–dung beetle seed dispersal system in Taï National Park, Côte d’Ivoire. The study focussed on the germination rate and success of four species of seeds contained in the faeces ofPan troglodytes verus: Dacryodes klaineana(Pierre) H.J. Lam,Diospyros manniiHiern,Pycnanthus angolensis(Welw.) Warb., andUapaca guineensisMuell. Arg. For each species, 600 seeds, half from chimpanzee faeces and half from mother trees, were sown in nurseries at depths of 0, 2, 5, 10, and 15 cm (i.e. 120 seeds per depth; 60 from chimp and 60 from mother trees). After germination, only the seeds ofUapaca guineensisandDiospyros manniisown at 2 and 5 cm had a >14% rate (between 14.2 and 30.8%) of germination and seedling establishment, regardless of seed origin. An increase in the depth of seed burial appears to negatively affect the probability of seedling emergence and establishment. This study shows that dung beetles have positive impacts on seed fate. However, for these plant species, the chimpanzee’s role is limited to that of primary seed dispersal.
Insiders, Outsiders, and Credible Visitors in Research
Authors: Porisky Alesha , Glas Aarie
Published: October 2022

There is a growing consensus around both the importance of researcher positionality for the conduct of research and the intersectional and variable salience of positionality and its effects. However, at the same time, static assumptions of “insider” and “outsider” status prevail. This article presents a productive and two-fold intervention in these discussions. First, we show that the insider/outsider distinction is fraught on logistical and conceptual grounds. Relying on our experiences in conducting interview research from rural villages to diplomatic offices, we show that these elements of status are fluid and dynamic. Second, we suggest an alternative to this dichotomy through the aspirational status of a “credible visitor.” We define this as a performative aspect of positionality founded on humility and reflexivity and enacted through showcasing competence and engaging in transparency. We describe how this approach to performing status may facilitate access as well as fruitful and ethical research interactions.
Olanzapine/samidorphan combination consistently mitigates weight gain across various subgroups of patients
Authors: Meyer Jonathan M. , Simmons Adam , Jiang Ying , Graham Christine , Yagoda Sergey , McDonnell David
Published: October 2022

Objective

A combination of olanzapine and the opioid receptor antagonist samidorphan (OLZ/SAM) has been approved in the United States for the treatment of adults with schizophrenia or adults with bipolar I disorder. In a phase 3 study in adults with schizophrenia (ENLIGHTEN-2), OLZ/SAM treatment was associated with significantly less weight gain compared with olanzapine. Prespecified subgroup analyses explored the consistency of the weight mitigation effect of OLZ/SAM vs olanzapine across demographic subgroups in ENLIGHTEN-2.

Methods

The multicenter, randomized, double-blind ENLIGHTEN-2 study (NCT02694328) included outpatients aged 18–55 years with a diagnosis of schizophrenia based on DSM-5 criteria, a body mass index (BMI) of 18 to 30 kg/m2, and stable body weight (self-reported change ≤5% for ≥3 months before study entry). Patients were randomized 1:1 to receive OLZ/SAM or olanzapine for 24 weeks. Co-primary endpoints (previously reported) were percent change in body weight and proportion of patients with at least 10% weight gain from baseline at week 24. Prespecified exploratory subgroup analyses by sex, age, self-reported race, and baseline BMI were conducted.

Results

At week 24, treatment with OLZ/SAM resulted in numerically less percent weight gain than with olanzapine across all subgroups evaluated. The proportion of patients with at least 10% weight gain was smaller in each subgroup treated with OLZ/SAM vs olanzapine.

Conclusion

In these exploratory subgroup analyses from the ENLIGHTEN-2 study, weight-mitigating effects of OLZ/SAM vs olanzapine were observed consistently across patient subgroups and were in line with results from the overall study population.
Specific pattern of linguistic impairment in Parkinson’s disease patients with subjective cognitive decline and mild cognitive impairment predicts dementia
Authors: Galtier Iván , Nieto Antonieta , Mata María , Lorenzo Jesús N. , Barroso José
Published: October 2022

Objective:

Parkinson’s disease patients with subjective cognitive decline (PD-SCD) and mild cognitive impairment (PD-MCI) have an increased risk of dementia (PDD). Thus, the identification of early cognitive changes that can be useful predictors of PDD is a highly relevant challenge. Posterior cortically based functions, including linguistic processes, have been associated with PDD. However, investigations that have focused on linguistic functions in PD-MCI are scarce and none of them include PD-SCD patients. Our aim was to study language performance in PD-SCD and PD-MCI. Moreover, language subcomponents were considered as predictors of PDD.

Method:

Forty-six PD patients and twenty controls were evaluated with a neuropsychological protocol. Patients were classified as PD-SCD and PD-MCI. Language production and comprehension was assessed. Follow-up assessment was conducted to a mean of 7.5 years after the baseline.

Results:

PD-MCI patients showed a poor performance in naming (actions and nouns), action generation, anaphora resolution and sentence comprehension (with and without center-embedded relative clause). PD-SCD showed a poor performance in action naming and action generation. Deficit in action naming was an independent risk factor for PDD during the follow-up. Moreover, the combination of deficit in action words and sentence comprehension without a center-embedded relative clause was associated with a greater risk.

Conclusions:

The results are of relevance because they suggest that a specific pattern of linguistic dysfunctions, that can be present even in the early stages of the disease, can predict future dementia, reinforcing the importance of advancing in the knowledge of linguistic dysfunctions in predementia stages of PD.
Patient–oncologist working alliance and its relation to locus of control
Authors: Levi Sivan , Amir Henny , Goldzweig Gil , Hasson-Ohayon Ilanit , Braun Michal
Published: October 2022

Objectives

In this study, we assessed the patient–oncologist relationship, conceptualized as the working alliance from a dyadic perspective, and its relation to locus of control.

Methods

One hundred and three oncologist–patient dyads were recruited. Measures included a sociodemographic and medical questionnaire; the “internal, powerful others, and chance” locus of control scale; and the working alliance inventory.

Results

Application of the actor–partner interdependence model yielded 2 actor effects: a positive association between oncologist “internal” locus of control and oncologist working alliance, and a negative association between oncologist “chance” locus of control and oncologist working alliance. It also yielded one partner effect: a positive association between oncologist “internal” locus of control and patient working alliance.

Significance of results

The actor–partner effect suggests that oncologists’ locus of control has a role in the establishment of the patient–oncologist working alliance; oncologists’ internal locus of control is a dominant factor affecting not only their own perceived alliance but patients’ perceived alliance as well.
Burnings, Beatings, and Bombings: Disaggregating Anti-Christian Violence in Egypt, 2013–2018
Authors: Barrie Christopher , Clarke Killian , Ketchley Neil
Published: October 2022

What are the determinants of ethnic violence? Existing research has forwarded a range of often competing explanations, from political opportunism to economic competition to state incapacity. We argue that this diversity of accounts is attributable, in part, to scholars’ tendency to lump together distinct forms of ethnic violence that have different underlying determinants. We propose that scholars instead disaggregate ethnic violence and put forward a typology based on the target of the attack (properties vs. individuals) and whether assailants use arms. We demonstrate the utility of this typology by applying it to an original dataset of ethnic attacks against Christians in Egypt from 2013 to 2018. In addition to a set of shared factors, we find that unarmed attacks against property (“burnings”) are the product of political mobilization, unarmed attacks against individuals (“beatings”) are related to socioeconomic tensions, and armed attacks (“bombings”) follow the strategic logic of terrorist violence.
Multi-criteria sustainability performance assessment of horticultural crops using DEA and ELECTRE IV methods
Authors: Banaeian Narges , Zangeneh Morteza , Golinska-Dawson Paulina
Published: October 2022

This paper presents a novel approach to multi-criteria sustainability performance assessment of horticultural crops. The crops are ranked by the decision-making method ELECTRE IV with environmental, energy and technological criteria. In total eight indicators are taken into consideration and calculated based on primary data collected from over 260 farms in northern Iran. Additionally, Data Envelopment Analysis is used to calculate the technical efficiency and potential for energy saving by different management of the production units. The novel contribution of this study is the comparison of several horticultural products (oranges, kiwis, persimmons and tangerines), when most of the previous studies have focused on one product. Moreover, novel calculations of the carbon footprint are presented for oranges, tangerines and persimmons. This paper also includes the first study on the environmental impact of persimmon fruit's production. The obtained results show that energy efficiency for orange, tangerine, kiwi and persimmon products: 1.1, 0.84, 0.53 and 1.22, respectively. In each hectare of kiwi orchards, the amount of CO2 emissions of 1219 kg and the ecological footprint of 3.21 hectares have been calculated, which is statistically significant compared to orange, tangerine and persimmon. The chemical and fuel inputs have the greatest potential for reducing energy consumption in the studied products. Results of ELECTRE IV showed that kiwi is the most sustainable selection for the studied region followed by orange, persimmon and tangerine, respectively. Kiwi has also relatively low technical efficiency. This means that this product has the greatest potential for a reduction of energy consumption, while maintaining the same amount of crop. It is recommended to include the development of kiwi orchards in the policies of Guilan, but with more careful management of the production inputs.
A Seismic Approach to Flood Detection and Characterization in Upland Catchments
Authors: Dietze M. , Hoffmann T. , Bell R. , Schrott L. , Hovius N.
Published: October 2022

About 10% of Europe's surface area is prone to rapid flooding of rivers confined in valleys. The devastating potential of such floods is exacerbated by the deficits of existing gauging networks, including low station densities and recording frequencies, and lack of information beyond stage height. Here, we use seismic data of the July 2021 Ahrtal flood, Germany, to extract information to complement sparse hydrometric data, and to reconstruct the rapid evolution of this fatal event. We show that a seismic station can deliver essential flood metrics such as magnitude, propagation velocity and debris transport rate. These seismic products provide high resolution insight to the non‐linear flood behavior. We argue that an approach combining distributed low‐cost seismometers with existing seismic stations, can provide important real time data on future catastrophic floods and associated hazards in upland catchments, offering precious response time also in currently ungauged landscapes.
Heavy Copper Isotopes in Arc‐Related Lavas From Cold Subduction Zones Uncover a Sub‐Arc Mantle Metasomatized by Serpentinite‐Derived Sulfate‐Rich Fluids
Authors: Chen Zuxing , Chen Jiubin , Tamehe Landry Soh , Zhang Yuxiang , Zeng Zhigang , Xia Xiaoping , Cui Zexian , Zhang Ting , Guo Kun
Published: October 2022

Serpentinite dehydration during subduction releases fluids into the sub‐arc mantle, yet the oxidizing capability of these fluids remains controversial. Here, redox‐sensitive Cu isotopes and in situ S isotopes of magmatic sulfides in fresh lavas from three subduction zones in the western Pacific were analyzed to determine the speciation of multi‐valence elements in the serpentinite‐derived fluids. Notably, the basaltic lavas from the Mariana Arc, eastern Manus Basin, and southern Okinawa Trough have isotopically heavy Cu (δ65Cu = +0.26 to +0.67‰) compared to the mantle‐like δ65Cu values of the middle Okinawa Trough and Mariana Trough basalts (+0.11 to +0.14‰). In addition, positive correlations between δ65Cu and slab fluid indicators (e.g., B/Nb, Ba/Th, and Ba/La) combined with positive δ34S values (∼+4‰) of magmatic sulfides in the Mariana Arc lavas, suggest that fluids with isotopically heavy Cu and S metasomatized their mantle sources. Theoretically, the bearing fluids released by serpentinite dehydration have extremely heavy Cu and S isotope compositions. Consequently, the sulfate‐rich fluids infiltration into the sub‐arc mantle produces heavy Cu and S isotopes in arc‐related magmas. Comparatively, sulfate‐rich fluids are absent at back‐arc depths due to the complete breakdown of serpentine minerals during the early stages of subduction, which results in the mid‐ocean ridge basalts‐like δ65Cu and δ34S values (∼−1‰) observed in the middle Okinawa Trough and Mariana Trough basalts. Therefore, heavy Cu isotopes in arc‐related magmas uncover that the sub‐arc mantle was metasomatized by serpentinite‐derived sulfate‐rich (oxidizing) fluids, which accounts for the oxidized nature of arc magmas in cold subduction zones.
Canyon Downwelling and Water Mass Influences on Winter Zooplankton Distributions in the Coastal Northeast Pacific
Authors: Howatt Tara , Ross Tetjana , Waterman Stephanie
Published: October 2022

Submarine canyons are considered biological hotspots due to enhanced mixing, upwelling, and other dynamic processes. There have been few observational surveys of canyon processes or zooplankton‐watermass relations during stormy downwelling seasons. In winter 2017, three autonomous underwater gliders provided highly‐resolved hydrographic and zooplankton backscatter observations in and around a canyon in the Northeast Pacific. While a glider's slow speed typically confounds spatial and temporal signals, the concurrent use of multiple gliders, sampling a canyon transect repeatedly, allowed us to explore the canyon system both spatially (i.e., canyon influence) and temporally (i.e., changing water type fractions). Canyon downwelling displaced the pycnocline by about 30 m depth over the scale of the canyon, which is ∼30% of the total pycnocline depth deviation observed over the course of the study. Diel vertically migrating (DVM) zooplankton generally stayed above the pycnocline and mean zooplankton volume scattering strength (Sv) in the upper water column (above the pycnocline) was higher when the pycnocline was deeper. At mid‐depths (150–200 m depth), zooplankton backscatter and DVM behavior were correlated with water type fractions, with higher Sv being associated with higher proportions of Pacific Equatorial Water and stronger DVM being associated with higher proportions of Pacific Subarctic Upper Water. Winter zooplankton distributions appear to be influenced by multiple, interacting drivers including canyon dynamics and water mass composition. In our study, the latter was found to have the stronger influence on zooplankton distribution.
Thermal Structure of the Middle and Upper Atmosphere of Mars From ACS/TGO CO2Spectroscopy
Authors: Belyaev D. A. , Fedorova A. A. , Trokhimovskiy A. , Alday J. , Korablev O. I. , Montmessin F. , Starichenko E. D. , Olsen K. S. , Patrakeev A. S.
Published: October 2022

Temperature and density in the upper Martian atmosphere, above ∼100 km, are key diagnostic parameters to study processes of the species' escape, investigate the impact of solar activity, model the atmospheric circulation, and plan spacecraft descent or aerobraking maneuvers. In this paper, we report vertical profiling of carbon dioxide (CO2) density and temperature from the Atmospheric Chemistry Suite (ACS) solar occultations onboard the ExoMars Trace Gas Orbiter. A strong CO2absorption band near 2.7 μm observed by the middle infrared spectrometric channel (ACS MIR) allows the retrieval of the atmospheric thermal structure in an unprecedentedly large altitude range, from 20 to 180 km. We present the latitudinal and seasonal climatology of the thermal structure for 1.5 Martian years (MYs), from the middle of MY 34 to the end of MY 35. The results show the variability of distinct atmospheric layers, such as a mesopause (derived from 70 to 145 km) and homopause, changing from 90 to 100 km at aphelion to 120–130 km at perihelion. Some short‐term homopause fluctuations are also observed depending on the dust activity.
Activation of glucagon-like peptide-1 receptors reduces the acquisition of aggression-like behaviors in male mice
Authors: Vestlund Jesper , Zhang Qian , Shevchouk Olesya T. , Hovey Daniel , Sebastian Lundström , Westberg Lars , Jerlhag Elisabet
Published: October 2022

Aggression is a complex social behavior, which is provoked in the defense of limited resources including food and mates. Recent advances show that the gut-brain hormone ghrelin modulates aggressive behaviors. As the gut-brain hormone glucagon-like peptide-1 (GLP-1) reduces food intake and sexual behaviors its potential role in aggressive behaviors is likely. Therefore, we investigated a tentative link between GLP-1 and aggressive behaviors by combining preclinical and human genetic-association studies. The influence of acute or repeated injections of a GLP-1 receptor (GLP-1R) agonist, exendin-4 (Ex4), on aggressive behaviors was assessed in male mice exposed to the resident-intruder paradigm. Besides, possible mechanisms participating in the ability of Ex4 to reduce aggressive behaviors were evaluated. Associations of polymorphisms inGLP-1Rgenes and overt aggression in males of the CATSS cohort were assessed. In male mice, repeated, but not acute, Ex4 treatment dose-dependently reduced aggressive behaviors. Neurochemical and western blot studies further revealed that putative serotonergic and noradrenergic signaling in nucleus accumbens, specifically the shell compartment, may participate in the interaction between Ex4 and aggression. As high-fat diet (HFD) impairs the responsiveness to GLP-1 on various behaviors the possibility that HFD blunts the ability of Ex4 to reduce aggressive behaviors was explored. Indeed, the levels of aggression was similar in vehicle and Ex4 treated mice consuming HFD. In humans, there were no associations between polymorphisms of theGLP-1Rgenes and overt aggression. Overall, GLP-1 signaling suppresses acquisition of aggressive behaviors via central neurotransmission and additional studies exploring this link are warranted.
Genetic deconvolution of fetal and maternal cell-free DNA in maternal plasma enables next-generation non-invasive prenatal screening
Authors: Xu Chenming , Li Jianli , Chen Songchang , Cai Xiaoqiang , Jing Ruilin , Qin Xiaomei , Pan Dong , Zhao Xin , Ma Dongyang , Xu Xiufeng , Liu Xiaojun , Wang Can , Yang Bingxin , Zhang Lanlan , Li Shuyuan , Chen Yiyao , Pan Nina , Tang Ping , Song Jieping , Liu Nian , Zhang Chen , Zhang Zhiwei , Qiu Xiang , Lu Weiliang , Ying Chunmei , Li Xiaotian , Xu Congjian , Wang Yanlin , Wu Yanting , Huang He-Feng , Zhang Jinglan
Published: October 2022

Current non-invasive prenatal screening (NIPS) analyzes circulating fetal cell-free DNA (cfDNA) in maternal peripheral blood for selected aneuploidies or microdeletion/duplication syndromes. Many genetic disorders are refractory to NIPS largely because the maternal genetic material constitutes most of the total cfDNA present in the maternal plasma, which hinders the detection of fetus-specific genetic variants. Here, we developed an innovative sequencing method, termed coordinative allele-aware target enrichment sequencing (COATE-seq), followed by multidimensional genomic analyses of sequencing read depth, allelic fraction, and linked single nucleotide polymorphisms, to accurately separate the fetal genome from the maternal background. Analytical confounders including multiple gestations, maternal copy number variations, and absence of heterozygosity were successfully recognized and precluded for fetal variant analyses. In addition, fetus-specific genomic characteristics, including the cfDNA fragment length, meiotic error origins, meiotic recombination, and recombination breakpoints were identified which reinforced the fetal variant assessment. In 1129 qualified pregnancies tested, 54 fetal aneuploidies, 8 microdeletions/microduplications, and 8 monogenic variants were detected with 100% sensitivity and 99.3% specificity. Using the comprehensive cfDNA genomic analysis tools developed, we found that 60.3% of aneuploidy samples had aberrant meiotic recombination providing important insights into the mechanism underlying meiotic nondisjunctions. Altogether, we show that the genetic deconvolution of the fetal and maternal cfDNA enables thorough and accurate delineation of fetal genome which paves the way for the next-generation prenatal screening of essentially all types of human genetic disorders.
INF2-mediated actin filament reorganization confers intrinsic resilience to neuronal ischemic injury
Authors: Calabrese Barbara , Jones Steven L. , Shiraishi-Yamaguchi Yoko , Lingelbach Michael , Manor Uri , Svitkina Tatyana M. , Higgs Henry N. , Shih Andy Y. , Halpain Shelley
Published: October 2022

During early ischemic brain injury, glutamate receptor hyperactivation mediates neuronal death via osmotic cell swelling. Here we show that ischemia and excess NMDA receptor activation cause actin to rapidly and extensively reorganize within the somatodendritic compartment. Normally, F-actin is concentrated within dendritic spines. However, <5 min after bath-applied NMDA, F-actin depolymerizes within spines and polymerizes into stable filaments within the dendrite shaft and soma. A similar actinification occurs after experimental ischemia in culture, and photothrombotic stroke in mouse. Following transient NMDA incubation, actinification spontaneously reverses. Na+, Cl−, water, and Ca2+ influx, and spine F-actin depolymerization are all necessary, but not individually sufficient, for actinification, but combined they induce activation of the F-actin polymerization factor inverted formin-2 (INF2). Silencing of INF2 renders neurons vulnerable to cell death and INF2 overexpression is protective. Ischemia-induced dendritic actin reorganization is therefore an intrinsic pro-survival response that protects neurons from death induced by cell edema.
The deglacial forest conundrum
Authors: Dallmeyer Anne , Kleinen Thomas , Claussen Martin , Weitzel Nils , Cao Xianyong , Herzschuh Ulrike
Published: October 2022

How fast the Northern Hemisphere (NH) forest biome tracks strongly warming climates is largely unknown. Regional studies reveal lags between decades and millennia. Here we report a conundrum: Deglacial forest expansion in the NH extra-tropics occurs approximately 4000 years earlier in a transient MPI-ESM1.2 simulation than shown by pollen-based biome reconstructions. Shortcomings in the model and the reconstructions could both contribute to this mismatch, leaving the underlying causes unresolved. The simulated vegetation responds within decades to simulated climate changes, which agree with pollen-independent reconstructions. Thus, we can exclude climate biases as main driver for differences. Instead, the mismatch points at a multi-millennial disequilibrium of the NH forest biome to the climate signal. Therefore, the evaluation of time-slice simulations in strongly changing climates with pollen records should be critically reassessed. Our results imply that NH forests may be responding much slower to ongoing climate changes than Earth System Models predict.
Traceless cysteine-linchpin enables precision engineering of lysine in native proteins
Authors: Reddy Neelesh C. , Molla Rajib , Joshi Pralhad Namdev , T. K. Sajeev , Basu Ipsita , Kawadkar Jyotsna , Kalra Neetu , Mishra Ram Kumar , Chakrabarty Suman , Shukla Sanjeev , Rai Vishal
Published: October 2022

The maintenance of machinery requires its operational understanding and a toolbox for repair. The methods for the precision engineering of native proteins meet a similar requirement in biosystems. Its success hinges on the principles regulating chemical reactions with a protein. Here, we report a technology that delivers high-level control over reactivity, chemoselectivity, site-selectivity, modularity, dual-probe installation, and protein-selectivity. It utilizes cysteine-based chemoselective Linchpin-Directed site-selective Modification of lysine residue in a protein (LDMC-K). The efficiency of the end-user-friendly protocol is evident in quantitative conversions within an hour. A chemically orthogonal C-S bond-formation and bond-dissociation are essential among multiple regulatory attributes. The method offers protein selectivity by targeting a single lysine residue of a single protein in a complex biomolecular mixture. The protocol renders analytically pure single-site probe-engineered protein bioconjugate. Also, it provides access to homogeneous antibody conjugates (AFC and ADC). The LDMC-K-ADC exhibits highly selective anti-proliferative activity towards breast cancer cells.
Single cell analysis of cribriform prostate cancer reveals cell intrinsic and tumor microenvironmental pathways of aggressive disease
Authors: Wong Hong Yuen , Sheng Quanhu , Hesterberg Amanda B. , Croessmann Sarah , Rios Brenda L. , Giri Khem , Jackson Jorgen , Miranda Adam X. , Watkins Evan , Schaffer Kerry R. , Donahue Meredith , Winkler Elizabeth , Penson David F. , Smith Joseph A. , Herrell S. Duke , Luckenbaugh Amy N. , Barocas Daniel A. , Kim Young J. , Graves Diana , Giannico Giovanna A. , Rathmell Jeffrey C. , Park Ben H. , Gordetsky Jennifer B. , Hurley Paula J.
Published: October 2022

Cribriform prostate cancer, found in both invasive cribriform carcinoma (ICC) and intraductal carcinoma (IDC), is an aggressive histological subtype that is associated with progression to lethal disease. To delineate the molecular and cellular underpinnings of ICC/IDC aggressiveness, this study examines paired ICC/IDC and benign prostate surgical samples by single-cell RNA-sequencing, TCR sequencing, and histology. ICC/IDC cancer cells express genes associated with metastasis and targets with potential for therapeutic intervention. Pathway analyses and ligand/receptor status model cellular interactions among ICC/IDC and the tumor microenvironment (TME) including JAG1/NOTCH. The ICC/IDC TME is hallmarked by increased angiogenesis and immunosuppressive fibroblasts (CTHRC1+ASPN+FAP+ENG+) along with fewer T cells, elevated T cell dysfunction, and increased C1QB+TREM2+APOE+-M2 macrophages. These findings support that cancer cell intrinsic pathways and a complex immunosuppressive TME contribute to the aggressive phenotype of ICC/IDC. These data highlight potential therapeutic opportunities to restore immune signaling in patients with ICC/IDC that may afford better outcomes.
Quorum-sensing control of matrix protein production drives fractal wrinkling and interfacial localization of Vibrio cholerae pellicles
Authors: Qin Boyang , Bassler Bonnie L.
Published: October 2022

Bacterial cells at fluid interfaces can self-assemble into collective communities with stunning macroscopic morphologies. Within these soft, living materials, called pellicles, constituent cells gain group-level survival advantages including increased antibiotic resistance. However, the regulatory and structural components that drive pellicle self-patterning are not well defined. Here, using Vibrio cholerae as our model system, we report that two sets of matrix proteins and a key quorum-sensing regulator jointly orchestrate the sequential mechanical instabilities underlying pellicle morphogenesis, culminating in fractal patterning. A pair of matrix proteins, RbmC and Bap1, maintain pellicle localization at the interface and prevent self-peeling. A single matrix protein, RbmA, drives a morphogenesis program marked by a cascade of ever finer wrinkles with fractal scaling in wavelength. Artificial expression of rbmA restores fractal wrinkling to a ΔrbmA mutant and enables precise tuning of fractal dimensions. The quorum-sensing regulatory small RNAs Qrr1-4 first activate matrix synthesis to launch pellicle primary wrinkling and ridge instabilities. Subsequently, via a distinct mechanism, Qrr1-4 suppress fractal wrinkling to promote fine modulation of pellicle morphology. Our results connect cell-cell signaling and architectural components to morphogenic patterning and suggest that manipulation of quorum-sensing regulators or synthetic control of rbmA expression could underpin strategies to engineer soft biomaterial morphologies on demand.
Chronic intake of high dietary sucrose induces sexually dimorphic metabolic adaptations in mouse liver and adipose tissue
Authors: Stephenson Erin J. , Stayton Amanda S. , Sethuraman Aarti , Rao Prahlad K. , Meyer Alice , Gomes Charles Klazer , Mulcahy Molly C. , McAllan Liam , Puchowicz Michelle A. , Pierre Joseph F. , Bridges Dave , Han Joan C.
Published: October 2022

Almost all effective treatments for non-alcoholic fatty liver disease (NAFLD) involve reduction of adiposity, which suggests the metabolic axis between liver and adipose tissue is essential to NAFLD development. Since excessive dietary sugar intake may be an initiating factor for NAFLD, we have characterized the metabolic effects of liquid sucrose intake at concentrations relevant to typical human consumption in mice. We report that sucrose intake induces sexually dimorphic effects in liver, adipose tissue, and the microbiome; differences concordant with steatosis severity. We show that when steatosis is decoupled from impairments in insulin responsiveness, sex is a moderating factor that influences sucrose-driven lipid storage and the contribution of de novo fatty acid synthesis to the overall hepatic triglyceride pool. Our findings provide physiologic insight into how sex influences the regulation of adipose-liver crosstalk and highlight the importance of extrahepatic metabolism in the pathogenesis of diet-induced steatosis and NAFLD.
Coemissive luminescent nanoparticles combining aggregation-induced emission and quenching dyes prepared in continuous flow
Authors: Li Chong , Liu Qi , Tao Shengyang
Published: October 2022

Achieving an ideal light-harvesting system at a low cost remains a challenge. Herein, we report the synthesis of a hybrid dye system based on tetraphenylene (TPE) encapsulated organic dyes in a continuous flow microreactor. The composite dye nanoparticles (NPs) are synthesized based on supramolecular self-assembly to achieve the co-emission of aggregation-induced emission dyes and aggregation-caused quenching dyes (CEAA). Numerical simulations and molecular spectroscopy were used to investigate the synthesis mechanism of the CEAA dyes. Nanoparticles of CEAA dyes provide a platform for efficient cascade Förster resonance energy transfer (FRET). Composite dye nanoparticles of TPE and Nile red (NiR) are synthesized for an ideal light-harvesting system using coumarin 6 (C-6) as an energy intermediate. The light-harvesting system has a considerable red-shift distance (~126 nm), high energy-transfer efficiency (ΦET) of 99.37%, and an antenna effect of 26.23. Finally, the versatility of the preparation method and the diversity of CEAA dyes are demonstrated.
Nonlinear germanium-silicon photodiode for activation and monitoring in photonic neuromorphic networks
Authors: Shi Yang , Ren Junyu , Chen Guanyu , Liu Wei , Jin Chuqi , Guo Xiangyu , Yu Yu , Zhang Xinliang
Published: October 2022

Silicon photonics is promising for artificial neural networks computing owing to its superior interconnect bandwidth, low energy consumption and scalable fabrication. However, the lack of silicon-integrated and monitorable optical neurons limits its revolution in large-scale artificial neural networks. Here, we highlight nonlinear germanium-silicon photodiodes to construct on-chip optical neurons and a self-monitored all-optical neural network. With specifically engineered optical-to-optical and optical-to-electrical responses, the proposed neuron merges the all-optical activation and non-intrusive monitoring functions in a compact footprint of 4.3 × 8 μm2. Experimentally, a scalable three-layer photonic neural network enables in situ training and learning in object classification and semantic segmentation tasks. The performance of this neuron implemented in a deep-scale neural network is further confirmed via handwriting recognition, achieving a high accuracy of 97.3%. We believe this work will enable future large-scale photonic intelligent processors with more functionalities but simplified architecture.
Algal photosystem I dimer and high-resolution model of PSI-plastocyanin complex
Authors: Naschberger Andreas , Mosebach Laura , Tobiasson Victor , Kuhlgert Sebastian , Scholz Martin , Perez-Boerema Annemarie , Ho Thi Thu Hoai , Vidal-Meireles André , Takahashi Yuichiro , Hippler Michael , Amunts Alexey
Published: October 2022

Photosystem I (PSI) enables photo-electron transfer and regulates photosynthesis in the bioenergetic membranes of cyanobacteria and chloroplasts. Being a multi-subunit complex, its macromolecular organization affects the dynamics of photosynthetic membranes. Here we reveal a chloroplast PSI from the green alga Chlamydomonas reinhardtii that is organized as a homodimer, comprising 40 protein subunits with 118 transmembrane helices that provide scaffold for 568 pigments. Cryogenic electron microscopy identified that the absence of PsaH and Lhca2 gives rise to a head-to-head relative orientation of the PSI–light-harvesting complex I monomers in a way that is essentially different from the oligomer formation in cyanobacteria. The light-harvesting protein Lhca9 is the key element for mediating this dimerization. The interface between the monomers is lacking PsaH and thus partially overlaps with the surface area that would bind one of the light-harvesting complex II complexes in state transitions. We also define the most accurate available PSI–light-harvesting complex I model at 2.3 Å resolution, including a flexibly bound electron donor plastocyanin, and assign correct identities and orientations to all the pigments, as well as 621 water molecules that affect energy transfer pathways.