Panax quinquefolium hairy root extracts and their effect in connections with antibiotics against pathogenic bacteria – preliminary study
Authors: Kochan Ewa 
Published: June 2019

<p class="western" style="margin-bottom: 0cm; line-height: 100%;" align="justify"> </p><p class="western" style="margin-bottom: 0cm; line-height: 100%;" align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en-US">The aim of the present study was to determine the level of ginsenosides in </span></span></span></span><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en-US">extracts from </span></span></span></span><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en-US">hairy root A, B, G clones of</span></span></span></span><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en-US"><em> Panax quinquefolium </em></span></span></span></span><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en-US">and their action with antibiotics against clinical bacterial isolates. The content of ginsenosides (the key biologically active compounds) were determined in tested extracts using HPLC. The activity of extracts with antibiotics was established by micro-dilution broth method. Total triterpene saponin content was 14.68, 14.32 and 10.07 mgg</span></span></span></span><span style="color: #000000;"><sup><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en-US">-1</span></span></span></sup></span><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en-US"> d.w. for root culture clones B, A and G, respectively. Our research indicates that the addition of extracts mainly from B and G clone hairy root cultures to antibiotics allow to reduce the ampicillin and tetracycline effective concentration respectively against </span></span></span></span><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en-US"><em>Enterococcus faecalis</em></span></span></span></span><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en-US"> and both </span></span></span></span><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en-US"><em>Escherichia coli</em></span></span></span></span><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en-US"> and </span></span></span></span><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en-US"><em>Acintobacter baumannii</em></span></span></span></span><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en">.</span></span></span></span></p><p class="western" style="margin-bottom: 0cm; line-height: 100%;" align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en"><br /></span></span></span></span></p>