bluuebunny/crossref_metadata_2025 huggingface dataset:
The Prognostic Value of the Surface Electromyographic Assessment of Pelvic Floor Muscles in Women with Stress Urinary Incontinence
Authors: Ptaszkowski Kuba , Malkiewicz Bartosz , Zdrojowy Romuald , Paprocka-Borowicz Malgorzata , Ptaszkowska Lucyna
Published: June 2020

Background: The use of surface electromyography (sEMG) measurements to evaluate the bioelectrical activity of the pelvic floor muscle (PFM) during therapeutic intervention is now well established. This study investigates the diagnostic possibilities of sEMG in women with stress urinary incontinence (SUI). The aim of this study was to carry out objective assessments of the bioelectrical activity of the PFM in women after menopause and determine the prognostic value of sEMG for assessing the PFM in patients with SUI. Methods: This was a prospective, observational study that evaluated the bioelectrical activity of the PFM in postmenopausal women with or without SUI (SUI group, n = 89 vs. non-SUI group, n = 62). The study was carried out between January 2013 and December 2018 at the Clinic of Urology (Wroclaw, Poland). The protocol for all sEMG measurements of PFM activity consisted of following elements: “baseline”, “quick flicks”, “contractions”, “static hold”, and “rest tone”; we then compared these results between groups. To determine the optimal cutoff level for sEMG activation of the PFM to detect the occurrence of SUI, we performed receiver operating characteristic (ROC) curve analysis (with Youden’s index). Results: Significantly lower results were obtained for all PFM measurements in women with SUI. The optimum diagnostic cutoff for “baseline” was 3.7 μV (area under curve (AUC), 0.63), “quick flicks” was 9.15 μV (AUC, 0.84), “contractions” was 11.33 μV (AUC, 0.80), “static hold” was 9.94 μV (AUC, 0.84), and “rest” was 3.89 μV (AUC, 0.63). Conclusions: Measuring sEMG activity in the PFM may be a useful diagnostic tool to confirm the absence of SUI. We can expect that the sEMG activity of subjects with SUI will be lower than that of healthy people. In order to determine appropriate reference values for assessing sEMG activity data in the PFM, it is now necessary to conduct multicenter studies.
Prospective Short-Term and Return-to-Sports Results of a Novel Uncemented Short-Stem Hip Prosthesis with Metaphyseal Anchorage
Authors: Breuer Robert , Fiala Rainer , Schrenk Nina , Tiefenboeck Thomas M.
Published: June 2020

Short-stem hip prostheses were developed to treat active patients requiring total hip arthroplasty (THA). This study provides short-term data about a short-stem total hip arthroplasty system. Functional and radiological outcomes as well as return to sports and activity level were assessed. A series of 55 patients was primarily included. Data were available for 47 patients at an average follow-up of 38 ± 4.6 months. The back-to-sports analysis showed a 98% return-to-sports rate (46/47 patients). The average time for return to sports was 13 weeks (± 8) postoperatively. Five patients (10.6%) were more active postoperatively. The Harris Hip Score (HHS) improved from 34.8 (±9.4) preoperatively to 94.7 (±8.4, p ≤ 0.001) and the University of California, Los Angeles (UCLA) score improved from 4.5 (±1.8) to 6.9 (±1.9) (p ≤ 0.001). The High Activity Arthroplasty Score (HAAS) was 12 (±3.6) at 3-year follow-up. Pre- and postoperative UCLA and postoperative HHS and HAAS scores had a positive influence on the return-to-sports rate (p ≤ 0.05). The collection of radiographic data during all postoperative follow-ups showed no signs of radiolucent lines or bone fissures. The complication rate was at 5%. Short-stem systems are equaling conventional prostheses and offer benefits regarding soft tissue and bone stock preservation. Fast recovery and return to sports can be achieved.
Dysregulation of microRNA Modulatory Network in Abdominal Aortic Aneurysm
Authors: Zalewski Daniel P. , Ruszel Karol P. , Stępniewski Andrzej , Gałkowski Dariusz , Bogucki Jacek , Komsta Łukasz , Kołodziej Przemysław , Chmiel Paulina , Zubilewicz Tomasz , Feldo Marcin , Kocki Janusz , Bogucka-Kocka Anna
Published: June 2020

Abdominal artery aneurysm (AAA) refers to abdominal aortic dilatation of 3 cm or greater. AAA is frequently underdiagnosed due to often asymptomatic character of the disease, leading to elevated mortality due to aneurysm rupture. MiRNA constitute a pool of small RNAs controlling gene expression and is involved in many pathologic conditions in human. Targeted panel detecting altered expression of miRNA and genes involved in AAA would improve early diagnosis of this disease. In the presented study, we selected and analyzed miRNA and gene expression signatures in AAA patients. Next, generation sequencing was applied to obtain miRNA and gene-wide expression profiles from peripheral blood mononuclear cells in individuals with AAA and healthy controls. Differential expression analysis was performed using DESeq2 and uninformative variable elimination by partial least squares (UVE-PLS) methods. A total of 31 miRNAs and 51 genes were selected as the most promising biomarkers of AAA. Receiver operating characteristics (ROC) analysis showed good diagnostic ability of proposed biomarkers. Genes regulated by selected miRNAs were determined in silico and associated with functional terms closely related to cardiovascular and neurological diseases. Proposed biomarkers may be used for new diagnostic and therapeutic approaches in management of AAA. The findings will also contribute to the pool of knowledge about miRNA-dependent regulatory mechanisms involved in pathology of that disease.
Disturbances of Continuous Sleep and Circadian Rhythms Account for Behavioral Difficulties in Children with Autism Spectrum Disorder
Authors: Yavuz-Kodat Enise , Reynaud Eve , Geoffray Marie-Maude , Limousin Nadège , Franco Patricia , Bonnet-Brilhault Frédérique , Bourgin Patrice , Schroder Carmen M.
Published: June 2020

Sleep disorders are among the most common comorbidities in children with Autism Spectrum Disorder (ASD), and subjectively defined sleep disturbances have been related to ASD symptom severity. However, no study has investigated the differential impact of objectively measured sleep and circadian rhythm disturbances on behavioral difficulties in this population. Fifty-two children with ASD aged 3–10 years underwent assessments of sleep and circadian rest–activity rhythms objectively with actigraphy and subjectively with the Children’s Sleep Habits Questionnaire. Behavioral difficulties were assessed using the ABC-C. Group comparison analyses were used to compare sleep and circadian rhythm parameters of children with higher and lower behavioral difficulties and dominance analysis to rank predictors and address multicollinearity. Children with high irritability had a shorter continuous sleep period compared to those with lower irritability (−60 min, p = 0.04), as well as those with high stereotypic behaviors compared to children with less stereotypies (−75 min, p = 0.006). Objective circadian and sleep disturbances accounted together for, respectively, 17%, 18% and 36% of the variance in social withdrawal, irritability and stereotypic behaviors. The identification of both sleep and circadian rhythm disturbances as explanatory factors for behavioral difficulties warrants their inclusion in the existing behavioral management strategies for children with ASD.
Inter-Hospital Transfer after Return of Spontaneous Circulation Shows no Correlation with Neurological Outcomes in Cardiac Arrest Patients Undergoing Targeted Temperature Management in Cardiac Arrest Centers
Authors: Choi Yoon Hee , Lee Dong Hoon , Oh Je Hyeok , Min Jin Hong , Jang Tae Chang , Kim Won Young , Jeong Won Jung , You Je Sung
Published: June 2020

This study evaluated whether inter-hospital transfer (IHT) after the return of spontaneous circulation (ROSC) was associated with poor neurological outcomes after 6 months in post-cardiac-arrest patients treated with targeted temperature management (TTM). We used data from the Korean Hypothermia Network prospective registry from November 2015 to December 2018. These out-of-hospital cardiac arrest (OHCA) patients had either received post-cardiac arrest syndrome (PCAS) care at the same hospital or had been transferred from another hospital after ROSC. The primary endpoint was the neurological outcome 6 months after cardiac arrest. Subgroup analyses were performed to determine differences in the time from ROSC to TTM induction according to the electrocardiography results after ROSC. We enrolled 1326 patients. There were no significant differences in neurological outcomes between the direct visit and IHT groups. In patients without ST elevation, the mean time to TTM was significantly shorter in the direct visit group than in the IHT group. IHT after achieving ROSC was not associated with neurologic outcomes after 6 months in post-OHCA patients treated with TTM, even though TTM induction was delayed in transferred patients.
Pre-Pregnancy Obesity, Excessive Gestational Weight Gain, and the Risk of Pregnancy-Induced Hypertension and Gestational Diabetes Mellitus
Authors: Lewandowska Małgorzata , Więckowska Barbara , Sajdak Stefan
Published: June 2020

Excessive pre-pregnancy weight is a known risk factor of pregnancy complications. The purpose of this analysis was to assess the relationship between several categories of maternal weight and the risk of developing hypertension and diabetes in pregnancy, and the relationship of these complications with the results of the newborn. It was carried out in a common cohort of pregnant women and taking into account the influence of disturbing factors. Our analysis was conducted in a prospective cohort of 912 Polish pregnant women, recruited during 2015–2016. We evaluated the women who subsequently developed diabetes with dietary modification (GDM-1) (n = 125) and with insulin therapy (GDM-2) (n = 21), as well as the women who developed gestational hypertension (GH) (n = 113) and preeclampsia (PE) (n = 24), compared to the healthy controls. Odds ratios of the complications (and confidence intervals (95%)) were calculated in the multivariate logistic regression. In the cohort, 10.8% of the women had pre-pregnancy obesity (body mass index (BMI) ≥ 30 kg/m2), and 36.8% had gestational weight gain (GWG) above the range of the Institute of Medicine recommendation. After correction for excessive GWG and other confounders, pre-pregnancy obesity (vs. normal BMI) was associated with a higher odds ratio of GH (AOR = 4.94; p < 0.001), PE (AOR = 8.61; p < 0.001), GDM-1 (AOR = 2.99; p < 0.001), and GDM-2 (AOR = 11.88; p <0.001). The threshold risk of development of GDM-2 occurred at lower BMI values (26.9 kg/m2), compared to GDM-1 (29.1 kg/m2). The threshold point for GH was 24.3 kg/m2, and for PE 23.1 kg/m2. For GWG above the range (vs. GWG in the range), the adjusted odds ratios of GH, PE, GDM-1, and GDM-2 were AOR = 1.71 (p = 0.045), AOR = 1.14 (p = 0.803), AOR = 0.74 (p = 0.245), and AOR = 0.76 (p = 0.672), respectively. The effect of maternal edema on all the results was negligible. In our cohort, hypertension and diabetes were associated with incorrect birth weight and gestational age at delivery. Conclusions: This study highlights the importance and influence of excessive pre-pregnancy maternal weight on the risk of pregnancy complications such as diabetes and hypertension which can impact fetal outcomes.
Morphometry of the Oculomotor Nerve in Duane’s Retraction Syndrome
Authors: Kang Min Seok , Yang Hee Kyung , Kim Jounghan , Kim Jae Hyoung , Hwang Jeong-Min
Published: June 2020

Objective: To investigate the morphometric characteristics of the oculomotor nerve and its association with horizontal rectus muscle volume in patients with Duane’s retraction syndrome (DRS) according to the presence of the abducens nerve. Methods: Fifty patients diagnosed with unilateral DRS were divided into two groups according to high-resolution magnetic resonance imaging (MRI) findings; DRS without an abducens nerve on the affected side (absent group, n = 41), and DRS with symmetric abducens nerves on both sides (present group, n = 9). Oculomotor nerve diameter was measured on high-resolution MRI in the middle of the cisternal space. The medial rectus muscle (MR) and lateral rectus muscle (LR) volumes were measured on T2-weighted coronal MRI of the orbit. Associations of oculomotor nerve diameter and horizontal rectus muscle volumes were performed according to the presence and absence of the abducens nerve. Results: Oculomotor nerve diameter on the affected side was thicker than that of the non-affected side in the absent group (p < 0.001), but not in the present group (p = 0.623). In the absent group, there was a positive correlation between oculomotor nerve diameter and MR volume (r = 0.779, p < 0.001), as well as the LR volume (r = 668, p = 0.023) of the affected eye. Conclusions: In DRS patients with an absent abducens nerve, the oculomotor nerve diameter was thicker in the affected eye compared to the non-affected eye. Oculomotor nerve diameter was associated with MR and LR volumes in the absent group. This study provides structural correlates of aberrant innervation of the oculomotor nerve in DRS patients.
Use of HER2-Directed Therapy in Metastatic Breast Cancer and How Community Physicians Collaborate to Improve Care
Authors: Mortimer Joanne E. , Kruper Laura , Cianfrocca Mary , Lavasani Sayeh , Liu Sariah , Tank-Patel Niki , Sedrak Mina , Smith Wade , Stewart Daphne , Waisman James , Yeon Christina , Wang Tina , Yuan Yuan
Published: June 2020

The development of new HER2-directed therapies has resulted in a significant prolongation of survival for women with metastatic HER2-positive breast cancer. Discoveries in the laboratory inform clinical trials which are the basis for improving the standard of care and are also the backbone for quality improvement. Clinical trials can be completed more rapidly by expanding trial enrollment to community sites. In this article we review some of the challenges in treating metastatic breast cancer with HER2-directed therapies and our strategies for incorporating our community partners into the research network.
Variable Stiffness Composites: Optimal Design Studies
Authors: Marques Filipe Eduardo Correia , Mota Ana Filipa Santos da , Loja Maria Amélia Ramos
Published: June 2020

This research work has two main objectives, being the first related to the characterization of variable stiffness composite plates’ behavior by carrying out a comprehensive set of analyses. The second objective aims at obtaining the optimal fiber paths, hence the characteristic angles associated to its definition, that yield maximum fundamental frequencies, maximum critical buckling loads, or minimum transverse deflections, both for a single ply and for a three-ply variable stiffness composite. To these purposes one considered the use of the first order shear deformation theory in connection to an adaptive single objective method. From the optimization studies performed it was possible to conclude that significant behavior improvements may be achieved by using variable stiffness composites. Hence, for simply supported three-ply laminates which were the cases where a major impact can be observed, it was possible to obtain a maximum transverse deflection decrease of 11.26%, a fundamental frequency increase of 5.61%, and a buckling load increase of 51.13% and 58.01% for the uniaxial and biaxial load respectively.
Severe Coastal Hypoxia Interchange with Ocean Acidification: An Experimental Perturbation Study on Carbon and Nutrient Biogeochemistry
Authors: Kapetanaki Natalia , Krasakopoulou Evangelia , Stathopoulou Eleni , Dassenakis Manos , Scoullos Michael
Published: June 2020

Normally atmospheric CO2 is the major driver of ocean acidification (OA); however, local discharge/degradation of organic matter (OM) and redox reactions can exacerbate OA in coastal areas. In this work we study the response of nutrient and carbon systems to pH decrease in relation to hydrographically induced intermittent characteristics and examine scenarios for future ocean acidification in a coastal system. Laboratory microcosm experiments were conducted using seawater and surface sediment collected from the deepest part of Elefsis Bay; the pH was constantly being monitored while CO2 gas addition was adjusted automatically. In Elefsis Bay surface pCO2 is already higher than global present atmospheric values, while near the bottom pCO2 reaches 1538 μatm and carbonate saturation states were calculated to be around 1.5. During the experiment, in more acidified conditions, limited alkalinity increase was observed and was correlated with the addition of bicarbonates and OM. Ammonium oxidation was decelerated and a nitrification mechanism was noticed, despite oxygen deficiency, paralleled by reduction of Mn-oxides. Phosphate was found significantly elevated for the first time in lower pH values, without reprecipitating after reoxygenation; this was linked with Fe(II) oxidation and Fe(III) reprecipitation without phosphate adsorption affecting both available dissolved phosphate and (dissolved inorganic nitrogen) DIN:DIP (dissolved inorganic phosphate)ratio.
Study on the Coastline Evolution in Sopot (2008–2018) Based on Landsat Satellite Imagery
Authors: Specht Mariusz , Specht Cezary , Lewicka Oktawia , Makar Artur , Burdziakowski Paweł , Dąbrowski Paweł
Published: June 2020

The coastline is the boundary between the water surface in a reservoir or watercourse and the land, which is characterised by high instability and functional diversity. For these reasons, research on coastal monitoring has been conducted for several decades. Currently, satellite images performed with synthetic aperture radars (SARs) are used to determine its course and variability together with high-resolution multispectral imagery from satellites such as IKONOS, QuickBird, and WorldView, or moderate-resolution multispectral images from Landsat satellites. This paper analysed the coastline variability in Sopot (2008–2018) based on Landsat satellite imagery. Furthermore, based on multispectral images obtained, it was determined how the beach surface in Sopot changed. Research has shown that the coastline keeps moving away from the land every year. This was particularly noticeable between 2008 and 2018 when the coastline moved on average 19.1 m towards the Baltic Sea. Moreover, it was observed that the area of the sandy beach in Sopot increased by 14 170.6 m2, which translates into an increase of 24.7% compared to 2008. The probable cause of the continuous coastline shift towards the sea and the increase of the beach surface is the oceanographic phenomenon called tombolo, which occurred in this area as a result of the construction of a yacht marina near the coast.
Sparrow: A Magnetic Climbing Robot for Autonomous Thickness Measurement in Ship Hull Maintenance
Authors: Enjikalayil Abdulkader Raihan , Veerajagadheswar Prabakaran , Htet Lin Nay , Kumaran Selva , Vishaal Suresh Raj , Mohan Rajesh Elara
Published: June 2020

The maintenance of ship hulls involves a series of routine tasks during dry-docking that renews its life-time and operating efficiency. One such task is hull inspection, which is always seen as harmful for human operators and a time-consuming task. The shipping maintenance industries started using the robotic solutions in order to reduce the human risk. However, most of such robotic systems cannot operate fully autonomously due to the fact that it requires humans in the loop. On the other hand, an autonomous hull inspection robot, called Sparrow, is presented in this paper. The proposed robot is capable of navigating autonomously on the vertical metal surface and it could perform metal thickness inspection. This article summarizes the robot’s mechanical design, system control, autonomy, and the inspection module. We evaluated the robot’s performance by conducting experimental trials on three different metal plates that varied in thickness. The results indicate that the presented robot achieves significantly better locomotion while climbing, and it can autonomously measure the metal thickness, which significantly reduces the human efforts in real-time.
Multiple TLDs on Motion Reduction Control of the Offshore Wind Turbines
Authors: Yeh Po-Hung , Chung Shao-Hua , Chen Bang-Fuh
Published: June 2020

This study explores the damping effects of tuned liquid dampers (TLDs) on a monopile offshore wind turbine (OWT). The fluid–solid coupling of ANSYS was used to simulate the damping effect of a TLD on the structures. The environmental conditions refer to the IEC-61400-3 and the Design Load Case (DLC) 1.2 for the annual average environmental conditions and DLC 6.2 for the 50-year regression period, and the extreme environmental conditions were used in the study. The turbulent wind field simulation was performed by TurbSim, and the load of wind waves on structures was generated by FAST, which were all developed by the NREL (National Renewable Energy Laboratory). In addition to wind and waves, the seismic force was also considered. The cylindrical TLD was located above the rotor nacelle assembly (RNA). A TLD has different damping effects when acting under wind, wave, and earthquake loads, respectively. The effect of the TLD regarding motion reduction on the OWT under coupled wind, wave, and seismic loads was studied. This study also designed a simple experiment to verify the correctness of the numerical simulation results. Fatigue analysis shows that multi-layer TLDs can extend the fatigue life (37%) of an OWT. In addition, under extreme environmental load conditions, multi-layer TLDs have a better vibration damping performance than single-layer TLDs. The study demonstrates that multi-layer TLDs can be considered as a vibration reduction damper for OWTs.
CyVerse Austria—A Local, Collaborative Cyberinfrastructure
Authors: Lang Konrad , Stryeck Sarah , Bodruzic David , Stepponat Manfred , Trajanoski Slave , Winkler Ursula , Lindstaedt Stefanie
Published: June 2020

Life sciences (LS) are advanced in research data management, since LS have established disciplinary tools for data archiving as well as metadata standards for data reuse. However, there is a lack of tools supporting the active research process in terms of data management and data analytics. This leads to tedious and demanding work to ensure that research data before and after publication are FAIR (findable, accessible, interoperable and reusable) and that analyses are reproducible. The initiative CyVerse US from the University of Arizona, US, supports all processes from data generation, management, sharing and collaboration to analytics. Within the presented project, we deployed an independent instance of CyVerse in Graz, Austria (CAT) in frame of the BioTechMed association. CAT helped to enhance and simplify collaborations between the three main universities in Graz. Presuming steps were (i) creating a distributed computational and data management architecture (iRODS-based), (ii) identifying and incorporating relevant data from researchers in LS and (iii) identifying and hosting relevant tools, including analytics software to ensure reproducible analytics using Docker technology for the researchers taking part in the initiative. This initiative supports research-related processes, including data management and analytics for LS researchers. It also holds the potential to serve other disciplines and provides potential for Austrian universities to integrate their infrastructure in the European Open Science Cloud.
Prognostic Factors for All-Cause Mortality in Thai Patients with Fragility Fracture of Hip: Comorbidities and Laboratory Evaluations
Authors: Atthakomol Pichitchai , Manosroi Worapaka , Phinyo Phichayut , Pipanmekaporn Tanyong , Vaseenon Tanawat , Rojanasthien Sattaya
Published: June 2020

Background and Objectives: Although the types of comorbidities and laboratory evaluations are major factors associated with mortality after hip fractures, there have been no studies of the association of these factors and mortality in Thai hip-fracture patients. This study aimed to identify prognostic factors associated with mortality after a hip fracture in the Thai population, including types of comorbidities, treatment-related factors, and laboratory evaluations. Materials and Methods: This five-year retrospective study was conducted in a tertiary care hospital in Thailand. A total of 775 Thai patients who had been admitted with a hip fracture resulting from a simple fall were identified using the International Classification of Disease 10 codes, and a review of their medical charts was conducted. Associations between general factors, comorbidities, laboratory evaluations, treatment factors including type of treatment, and time to death were analyzed using the Cox proportional hazard regression and the hazard ratio (HR). Results: The overall mortality rate of hip fracture patients was 13.94%. Independent prognostic factors found to be significantly associated with mortality were nonoperative treatment (HR = 3.29, p < 0.001), admission glomerular filtration rate (GFR) < 30 mL/min/1.73 m2 (HR = 3.40, p < 0.001), admission hemoglobin concentration <10 g/dL. (HR = 2.31, p < 0.001), chronic obstructive pulmonary disorder (HR = 2.63, p < 0.001), dementia or Alzheimer’s disease (HR = 4.06, p < 0.001), and active malignancy (HR = 6.80, p < 0.001). Conclusion: The types of comorbidities and laboratory evaluation findings associated with mortality in Thai patients with hip fractures include chronic obstructive pulmonary disorder, dementia or Alzheimer’s disease, active malignancy, admission GFR < 30 mL/min/1.73 m2, and admission hemoglobin concentration <10 g/dL. The risks of mortality for Thai hip-fracture patients with these comorbidities or laboratory evaluation findings were 2.5, 4, 7, 3.5, and 2.5 times higher, respectively, than patients without those factors.
Osteopathic Manipulative Treatment in Neonatal Intensive Care Units
Authors: Cicchitti Luca , Di Lelio Alessandra , Barlafante Gina , Cozzolino Vincenzo , Di Valerio Susanna , Fusilli Paola , Lucisano Giuseppe , Renzetti Cinzia , Verzella Marco , Rossi Maria Chiara
Published: June 2020

The aim of this study was to assess the impact of osteopathic manipulative treatment (OMT) on newborn babies admitted at a neonatal intensive care unit (NICU). This was an observational, longitudinal, retrospective study. All consecutive admitted babies were analyzed by treatment (OMT vs. usual care). Treatment group was randomly assigned. Between-group differences in weekly weight change and length of stay (LOS) were evaluated in the overall and preterm populations. Among 1249 babies (48.9% preterm) recorded, 652 received usual care and 597 received OMT. Weight increase was more marked in the OMT group than in the control group (weekly change: +83 g vs. +35 g; p < 0.001). Similar trends were found in the subgroup of preterm babies. A shorter LOS was found in the OMT group vs. the usual care group both in overall population (average mean difference: −7.9 days, p = 0.15) and in preterm babies (−12.3 days; p = 0.04). In severe preterm babies, mean LOS was more than halved as compared to the control group. OMT was associated with a more marked weekly weight increase and, especially in preterm babies, to a relevant LOS reduction: OMT may represent an efficient support to usual care in newborn babies admitted at a NICU.
Diffusion Dialysis for Separation of Hydrochloric Acid, Iron and Zinc Ions from Highly Concentrated Pickling Solutions
Authors: Gueccia Rosa , Aguirre Alba Ruiz , Randazzo Serena , Cipollina Andrea , Micale Giorgio
Published: June 2020

Acid recovery from pickling waste solutions is an important step to enhance hot-dip-galvanizing industry process sustainability. Diffusion dialysis (DD) can be used to separate acids and heavy metals (e.g., iron and zinc) from pickling waters, promoting the circular use of such raw materials. In the present study, a laboratory scale unit operating in batch and a continuous large scale unit, both equipped with Fumasep anionic exchange membranes, were tested. Results obtained show that zinc and iron concentration affect the HCl recovery in opposite ways. Iron chlorides enhance acid recovery, while zinc chlorides considerably tend to diffuse through the membrane because of negatively charged chloro-complexes formation and slightly reduce the acid diffusion. A multi-components mathematical model, with a time-dependent and distributed-parameters architecture, was adopted enabling the prediction of operations with hydrochloric acid, zinc, and iron metals both in batch and in continuous dialyzers. As a result, a good comparison between model simulations and experiments was achieved in both configurations.
Mechanism and Kinetics of Malachite Dissolution in an NH4OH System
Authors: Aracena Alvaro , Pino Javiera , Jerez Oscar
Published: June 2020

Copper oxide minerals composed of carbonates consume high quantities of leaching reagent. The present research proposes an alternative procedure for malachite leaching (Cu2CO3(OH)2) through the use of only compound, ammonium hydroxide (NH4OH). Preliminary studies were also carried out for the dissolution of malachite in an acid system. The variables evaluated were solution pH, stirring rate, temperature, NH4OH concentration, particle size, solid/liquid ratio and different ammonium reagents. The experiments were carried out in a stirred batch system with controlled temperatures and stirring rates. For the acid dissolution system, sulfuric acid consumption reached excessive values (986 kg H2SO4/ton of malachite), invalidating the dissolution in these common systems. On the other hand, for the ammoniacal system, there was no acid consumption and the results show that copper recovery was very high, reaching values of 84.1% for a concentration of 0.2 mol/dm3 of NH4OH and an experiment time of 7200 s. The theoretical/thermodynamic calculations indicate that the solution pH was a significant factor in maintaining the copper soluble as Cu(NH3)42+. This was validated by the experimental results and solid analysis by X-ray diffraction (XRD), from which the reaction mechanisms were obtained. A heterogeneous kinetic model was obtained from the diffusion model in a porous layer for particles that begin the reaction as nonporous but which become porous during the reaction as the original solid splits and cracks to form a highly porous structure. The reaction order for the NH4OH concentration was 3.2 and was inversely proportional to the square of the initial radius of the particle. The activation energy was calculated at 36.1 kJ/mol in the temperature range of 278 to 313 K.
Tool Performance Optimization While Machining Aluminium-Based Metal Matrix Composite
Authors: Umer Usama , Abidi Mustufa Haider , Qudeiri Jaber Abu , Alkhalefah Hisham , Kishawy Hossam
Published: June 2020

Finite element (FE) models and the multi objective genetic algorithm (MOGA-II) have been applied for tool performance optimization while machining aluminum-based metal matrix composites. The developed and verified FE models are utilized to generate data for the full factorial design of experiment (DOE) plan. The FE models consist of a heterogenous workpiece, which assumes uniform distribution of reinforced particles according to size and volume fraction. Cutting forces, chip morphology, temperature contours, stress distributions in the workpiece and tool by altering cutting speed, feed rate, and reinforcement particle size can be estimated using developed FE models. The DOE data are then utilized to develop response surfaces using radial basis functions. To reduce computational time, these response surfaces are used as solver for optimization runs using MOGA-II. Tool performance has been optimized with regard to cutting temperatures and stresses while setting a limit on specific cutting energy. Optimal solutions are found with low cutting speed and moderate feed rates for each particle size metal matrix composite (MMC).
Mechanical Rupture-Based Antibacterial and Cell-Compatible ZnO/SiO2 Nanowire Structures Formed by Bottom-Up Approaches
Authors: Shimada Taisuke , Yasui Takao , Yonese Akihiro , Yanagida Takeshi , Kaji Noritada , Kanai Masaki , Nagashima Kazuki , Kawai Tomoji , Baba Yoshinobu
Published: June 2020

There are growing interests in mechanical rupture-based antibacterial surfaces with nanostructures that have little toxicity to cells around the surfaces; however, current surfaces are fabricated via top-down nanotechnologies, which presents difficulties to apply for bio-surfaces with hierarchal three-dimensional structures. Herein, we developed ZnO/SiO2 nanowire structures by using bottom-up approaches and demonstrated to show mechanical rupture-based antibacterial activity and compatibility with human cells. When Escherichia coli were cultured on the surface for 24 h, over 99% of the bacteria were inactivated, while more than 80% of HeLa cells that were cultured on the surface for 24 h were still alive. This is the first demonstration of mechanical rupture-based bacterial rupture via the hydrothermally synthesized nanowire structures with antibacterial activity and cell compatibility.
Characterization of the Phenanthrene-Degrading Sphingobium yanoikuyae SJTF8 in Heavy Metal Co-Existing Liquid Medium and Analysis of Its Metabolic Pathway
Authors: Yin Chong , Xiong Weiliang , Qiu Hua , Peng Wanli , Deng Zixin , Lin Shuangjun , Liang Rubing
Published: June 2020

Polycyclic aromatic hydrocarbons (PAHs) are common organic pollutants with great carcinogenic threaten, and metal/PAH-contaminated environments represent one of the most difficult remedial challenges. In this work, Sphingobium yanoikuyae SJTF8 was isolated and identified with great and stable PAH-degrading efficiency even under stress conditions. It could utilize typical PAHs (naphthalene, phenanthrene, and anthracene) and heterocyclic and halogenated aromatic compounds (dibenzothiophene and 9-bromophenanthrene) as the sole carbon source. It could degrade over 98% of 500 mg/L phenanthrene in 4 days, and the cis-3,4-dihydrophenanthrene-3,4-diol was the first-step intermediate. Notably, strain SJTF8 showed great tolerance to heavy metals and acidic pH. Supplements of 0.30 mM of Cu2+, 1.15 mM of Zn2+, and 0.01 mM of Cd2+ had little effect on its cell growth and phenanthrene degradation; phenanthrene of 250 mg/L could still be degraded completely in 48 h. Further, the whole genome sequence of S. yanoikuyae SJTF8 was obtained, and three plasmids were found. The potential genes participating in stress-tolerance and PAH-degradation were annotated and were found mostly distributed in plasmids 1 and 2. Elimination of plasmid 2 resulted in the loss of the PAH-degradation ability. On the basis of genome mining results, the possible degrading pathway and the metabolites of S. yanoikuyae SJTF8 to phenanthrene were predicted.
First Demonstration of Clinical Fusarium Strains Causing Cross-Kingdom Infections from Humans to Plants
Authors: Meza-Menchaca Thuluz , Singh Rupesh Kumar , Quiroz-Chávez Jesús , García-Pérez Luz María , Rodríguez-Mora Norma , Soto-Luna Manuel , Gastélum-Contreras Guadalupe , Vanzzini-Zago Virginia , Sharma Lav , Quiroz-Figueroa Francisco Roberto
Published: June 2020

Mycotoxins from the Fusarium genus are widely known to cause economic losses in crops, as well as high mortalities rates among immunocompromised humans. However, to date, no correlation has been established for the ability of Fusarium to cause cross-kingdom infection between plants and humans. The present investigation aims to fill this gap in the literature by examining cross-kingdom infection caused by Furasium strains isolated from non-immunocompromised or non-immunosuppressed humans, which were subsequently reinfected in plants and on human tissue. The findings document for the first time cross-kingdom infective events in Fusarium species, thus enhancing our existing knowledge of how mycopathogens continue to thrive in different hosts.
Plant Growth Enhancement using Rhizospheric Halotolerant Phosphate Solubilizing Bacterium Bacillus licheniformis QA1 and Enterobacter asburiae QF11 Isolated from Chenopodium quinoa Willd
Authors: Mahdi Ismail , Fahsi Nidal , Hafidi Mohamed , Allaoui Abdelmounaaim , Biskri Latefa
Published: June 2020

Plant growth-promoting rhizobacteria represent a promising solution to enhancing agricultural productivity. Here, we screened phosphate solubilizing bacteria from the rhizospheric soil of Chenopodium quinoa Willd and assessed their plant-growth promoting rhizobacteria (PGPR) properties including production of indole-3-acetic acid (IAA), siderophores, hydrogen cyanide (HCN), ammonia and extracellular enzymes. We also investigated their tolerance to salt stress and their capacity to form biofilms. Two isolated strains, named QA1 and QF11, solubilized phosphate up to 346 mg/L, produced IAA up to 795.31 µg/mL, and tolerated up to 2 M NaCl in vitro. 16S rRNA and Cpn60 gene sequencing revealed that QA1 and QF11 belong to the genus Bacillus licheniformis and Enterobacter asburiae, respectively. In vivo, early plant growth potential showed that quinoa seeds inoculated either with QA1 or QF11 displayed higher germination rates and increased seedling growth. Under saline irrigation conditions, QA1 enhanced plant development/growth. Inoculation with QA1 increased leaf chlorophyll content index, enhanced P and K+ uptake and decreased plant Na+ uptake. Likewise, plants inoculated with QF11 strain accumulated more K+ and had reduced Na+ content. Collectively, our findings support the use of QA1 and QF11 as potential biofertilizers.
Novel Antifungal Activity of Lolium-Associated Epichloë Endophytes
Authors: Fernando Krishni , Reddy Priyanka , Hettiarachchige Inoka K. , Spangenberg German C. , Rochfort Simone J. , Guthridge Kathryn M.
Published: June 2020

Asexual Epichloë spp. fungal endophytes have been extensively studied for their functional secondary metabolite production. Historically, research mostly focused on understanding toxicity of endophyte-derived compounds on grazing livestock. However, endophyte-derived compounds also provide protection against invertebrate pests, disease, and other environmental stresses, which is important for ensuring yield and persistence of pastures. A preliminary screen of 30 strains using an in vitro dual culture bioassay identified 18 endophyte strains with antifungal activity. The novel strains NEA12, NEA21, and NEA23 were selected for further investigation as they are also known to produce alkaloids associated with protection against insect pests. Antifungal activity of selected endophyte strains was confirmed against three grass pathogens, Ceratobasidium sp., Dreschlera sp., and Fusarium sp., using independent isolates in an in vitro bioassay. NEA21 and NEA23 showed potent activity against Ceratobasidium sp. and NEA12 showed moderate inhibition against all three pathogens. Crude extracts from liquid cultures of NEA12 and NEA23 also inhibited growth of the phytopathogens Ceratobasidium sp. and Fusarium sp. and provided evidence that the compounds of interest are stable, constitutively expressed, and secreted. Comparative analysis of the in vitro and in planta metabolome of NEA12 and NEA23 using LCMS profile data revealed individual metabolites unique to each strain that are present in vitro and in planta. These compounds are the best candidates for the differential bioactivity observed for each strain. Novel endophyte strains show promise for endophyte-mediated control of phytopathogens impacting Lolium spp. pasture production and animal welfare.
Early Ediacaran Magmatism in the Yenisei Ridge and Evolution of the Southwestern Margin of the Siberian Craton
Authors: Vernikovskaya Antonina , Vernikovsky Valery A. , Matushkin Nikolay , Kadilnikov Pavel , Metelkin Dmitry V. , Romanova Irina , Li Zheng-Xiang , Bogdanov Egor
Published: June 2020

We present the first comprehensive data for the early Ediacaran stage of evolution of the western active continental margin of the Siberian Craton (Yenisei Ridge). U-Pb (SHRIMP-II SIMS) data for zircons from dikes of picrodolerites, quartz diorites, and leucocratic granites show that they were emplaced at 625 ± 5, 623 ± 8, and 626 ± 5 Ma, respectively, which indicates a narrow time window of Ediacaran magmatic events. The mafic tholeiitic rocks have OIB and E-MORB mantle components in their magmatic sources. Mineralogical and geochemical observations showed that the mantle-sourced mafic melts assimilated some crustal material, forming Th-enriched dikes of intermediate composition and K- and Rb-enriched felsic rocks. The possible geodynamic conditions for the formation of these early Ediacaran dikes are shown and a geodynamic model is presented for the development of the Yenisei Ridge orogen from the late Cryogenian to the late Ediacaran.
Quantitative Analysis of Asbestos-Containing Materials Using Various Test Methods
Authors: Yang Kiho , Yoo Kyu-Cheul , Jung Jaewoo
Published: June 2020

The advantages of X-ray powder diffraction (XRPD) analysis are its non-destructive nature, reliability, fast and easy sample preparation, and low costs. XRPD analysis has been used for mineral identification and the quantitative/qualitative determination of various types of fibrous minerals in asbestos-containing materials (ACMs). In order to test the detection limit of ACMs by XRPDD, standard samples with various concentrations of ACMs (0.1%, 1%, and 3%) were fabricated using three matrix materials (talc, vermiculite, and sepiolite). Asbestiform tremolite and chrysotile were identified in the XRPD profiles of the samples with 1% and 3% ACMs. Their integral intensities were positively correlated with the concentrations. However, the XRPD peak of asbestos was not found in the samples with 0.1% ACMs. Therefore, scanning and transmission electron microscopy were utilized to investigate the samples with a very low concentration of ACMs. Although the ACM concentration (0.1%) was negligible and its direct observation was time-consuming, electron microscopy allowed for the detection of asbestos in several matrix materials. Thus, a combination of XRPD and electron microscopy improve analytical performance and data reliability.
Epigallocatechin-3-Gallate (EGCG), an Active Compound of Green Tea Attenuates Acute Lung Injury Regulating Macrophage Polarization and Krüpple-Like-Factor 4 (KLF4) Expression
Authors: Almatroodi Saleh A. , Almatroudi Ahmad , Alsahli Mohammed A. , Aljasir Mohammad A. , Syed Mansoor Ali , Rahmani Arshad Husain
Published: June 2020

Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) are serious clinical complications with a high frequency of morbidity and mortality. The initiation and amplification of inflammation is a well-known aspect in the pathogenesis of ALI and related disorders. Therefore, inhibition of the inflammatory mediators could be an ideal approach to prevent ALI. Epigallocatechin-3-gallate (EGCG), a major constituent of green tea, has been shown to have protective effects on oxidative damage and anti-inflammation. The goal of the present study was to determine whether EGCG improves phenotype and macrophage polarisation in LPS-induced ALI. C57BL/6 mice were given two doses of EGCG (15 mg/kg) intraperitoneally (IP) 1 h before and 3 h after LPS instillation (2 mg/kg). EGCG treatment improved histopathological lesions, Total Leucocyte count (TLC), neutrophils infiltration, wet/dry ratio, total proteins and myeloperoxidase (MPO) activity in LPS-induced lung injury. The results displayed that EGCG reduced LPS-induced ALI as it modulates macrophage polarisation towards M2 status. Furthermore, EGCG also reduced the expression of proinflammatory M1 mediators iNOS TNF-α, IL-1β and IL-6 in the LPS administered lung microenvironment. In addition, it increased the expression of KLF4, Arg1 and ym1, known to augment the M2 phenotype of macrophages. EGCG also alleviated the expression of 8-OHdG, nitrotyrosine, showing its ability to inhibit oxidative damage. TREM1 in the lung tissue and improved lung regenerative capacity by enhancing Ki67, PCNA and Ang-1 protein expression. Together, these results proposed the protective properties of EGCG against LPS-induced ALI in may be attributed to the suppression of M1/M2 macrophages subtype ratio, KLF4 augmentation, lung cell regeneration and regulating oxidative damage in the LPS-induced murine ALI.
Flavonoids in Decorticated Sorghum Grains Exert Antioxidant, Antidiabetic and Antiobesity Activities
Authors: Ofosu Fred Kwame , Elahi Fazle , Daliri Eric Banan-Mwine , Yeon Su-Jung , Ham Hun Ju , Kim Joong-Hark , Han Sang-Ik , Oh Deog-Hwan
Published: June 2020

Eight new genotypes of brown sorghum grain were decorticated and assessed for their antioxidant, antidiabetic and antiobesity activities in vitro. The DPPH and ABTS radical scavenging assays of the soluble fractions were evaluated, followed by digestive enzymes and advanced glycation end-products (AGEs) formation inhibition assays. DSOR 33 and DSOR 11 exhibited the highest DPPH (IC50 = 236.0 ± 1.98 µg/mL and 292.05 ± 2.19 µg/mL, respectively) and ABTS radical scavenging activity (IC50 = 302.50 ± 1.84 µg/mL and 317.05 ± 1.06 µg/mL, respectively). DSOR 17, DSOR 11 and DSOR 33 showed significantly higher inhibitory activity of both α-glucosidase and α-amylase (IC50 = 31.86, 35.10 and 49.40 µg/mL; and 15.87, 22.79 and 37.66 µg/mL, respectively) compared to acarbose (IC50 = 59.34 and 27.73 µg/mL, respectively). Similarly, DSOR 33, DSOR 11 and DSOR 17 showed potent inhibition of both AGEs and lipase with IC50 values of 18.25, 19.03 and 38.70 µg/mL; and 5.01, 5.09 and 4.94 µg/mL, respectively, compared to aminoguanidine (52.30 µg/mL) and orlistat (5.82 µg/mL). Flavonoids were the predominant compounds identified, with flavones being the major subclass in these three extracts. Our findings suggest that decorticated sorghum grains contain substantial amounts of flavonoids and could be promising candidates for the prevention and treatment of diabetes and obesity.
Antibody-Drug Conjugates and Targeted Treatment Strategies for Hepatocellular Carcinoma: A Drug-Delivery Perspective
Authors: Dahlgren David , Lennernäs Hans
Published: June 2020

Increased understanding of cancer biology, pharmacology and drug delivery has provided a new framework for drug discovery and product development that relies on the unique expression of specific macromolecules (i.e., antigens) on the surface of tumour cells. This has enabled the development of anti-cancer treatments that combine the selectivity of antibodies with the efficacy of highly potent chemotherapeutic small molecules, called antibody-drug conjugates (ADCs). ADCs are composed of a cytotoxic drug covalently linked to an antibody which then selectively binds to a highly expressed antigen on a cancer cell; the conjugate is then internalized by the cell where it releases the potent cytotoxic drug and efficiently kills the tumour cell. There are, however, many challenges in the development of ADCs, mainly around optimizing the therapeutic/safety benefits. These challenges are discussed in this review; they include issues with the plasma stability and half-life of the ADC, its transport from blood into and distribution throughout the tumour compartment, cancer cell antigen expression and the ADC binding affinity to the target antigen, the cell internalization process, cleaving of the cytotoxic drug from the ADC, and the cytotoxic effect of the drug on the target cells. Finally, we present a summary of some of the experimental ADC strategies used in the treatment of hepatocellular carcinoma, from the recent literature.