bluuebunny/crossref_metadata_2025 huggingface dataset:
Synthesis of Berberine and Canagliflozin Chimera and Investigation into New Antibacterial Activity and Mechanisms
Authors: Hao Wenhui , Che Shiying , Li Jinsheng , Luo Jingyi , Zhang Wanqiu , Chen Yang , Zhao Zijian , Wei Hao , Xie Weidong
Published: May 2022

Berberine is an isoquinoline alkaloid isolated from Chinese herbal medicines such as Coptis chinensis. It has many pharmacological actions, such as antibacterial, hypoglycemic, anti-inflammatory, and so on. However, due to the low lipophilicity of berberine, it is difficult to penetrate the bacterial cell membrane and also difficult to be absorbed orally and usually needs a relatively high dose to achieve the ideal effect. The purpose of this study is to transform the structure of berberine in order to improve the bioavailability of berberine and reduce the dosage. Moreover, we introduce a pharmacophore named Canagliflozin, a hypoglycemic drug (which was also found to have potential anti-bacterial activity) into BBR to see whether this new compound has more existed activities. We at first connected berberine with Canagliflozin, to form a new compound (BC) and see whether BC has synergic effects. We use microbroth dilution method to determine the minimum inhibitory concentration of BC, determine the bacterial growth with the enzyme labeling instrument, observe the formation of bacterial biofilm with crystal violet staining method, observe the bacterial morphology with field emission scanning electron microscope, and determine the intracellular protein with SDS-PAGE. The above indicators reflect the damage of BC to bacteria. New compound BC was successfully obtained by chemical synthesis. The minimal inhibitory concentration of compound BC on three bacteria was significantly better than that of berberine and canagliflozin alone and the combination of berberine and canagliflozin. Moreover, compound BC has obvious destructive effect on bacterial morphology and biofilm, and the compound also has destructive effect on intracellular proteins. Therefore, new compound BC has broad-spectrum antibacterial activity and the inhibitory effect of BC might play a role by destroying the integrity of biofilm and the intracellular protein of bacteria. In conclusion, we create a new molecular entity of berberine and Canagliflozin chimera and open up a new prospect for berberine derivatives in the treatment of bacterial infection.
Correction: Rubnawaz et al. Polyphenol Rich Ajuga bracteosa Transgenic Regenerants Display Better Pharmacological Potential. Molecules 2021, 26, 4874
Authors: Rubnawaz Samina , Kayani Waqas Khan , Akhtar Nosheen , Mahmood Rashid , Khan Asif , Okla Mohammad K. , Alamri Saud A. , Alaraidh Ibrahim A. , Alwasel Yasmeen A. , Mirza Bushra
Published: May 2022

The authors of this paper [...]
Green Extraction Techniques as Advanced Sample Preparation Approaches in Biological, Food, and Environmental Matrices: A Review
Authors: Câmara José S. , Perestrelo Rosa , Berenguer Cristina V. , Andrade Carolina F. P. , Gomes Telma M. , Olayanju Basit , Kabir Abuzar , M. R. Rocha Cristina , Teixeira José António , Pereira Jorge A. M.
Published: May 2022

Green extraction techniques (GreETs) emerged in the last decade as greener and sustainable alternatives to classical sample preparation procedures aiming to improve the selectivity and sensitivity of analytical methods, simultaneously reducing the deleterious side effects of classical extraction techniques (CETs) for both the operator and the environment. The implementation of improved processes that overcome the main constraints of classical methods in terms of efficiency and ability to minimize or eliminate the use and generation of harmful substances will promote more efficient use of energy and resources in close association with the principles supporting the concept of green chemistry. The current review aims to update the state of the art of some cutting-edge GreETs developed and implemented in recent years focusing on the improvement of the main analytical features, practical aspects, and relevant applications in the biological, food, and environmental fields. Approaches to improve and accelerate the extraction efficiency and to lower solvent consumption, including sorbent-based techniques, such as solid-phase microextraction (SPME) and fabric-phase sorbent extraction (FPSE), and solvent-based techniques (μQuEChERS; micro quick, easy, cheap, effective, rugged, and safe), ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE), in addition to supercritical fluid extraction (SFE) and pressurized solvent extraction (PSE), are highlighted.
Non-Invasive Detection of Anti-Inflammatory Bioactivity and Key Chemical Indicators of the Commercial Lanqin Oral Solution by Near Infrared Spectroscopy
Authors: Ma Hui , Xiao Lulu , Xu Dongchen , Geng Yingrui , Liu Xuesong , Chen Yong , Wu Yongjiang
Published: May 2022

Quality control methods of current traditional Chinese medicine (TCM) preparation is time-consuming and difficult to assess in terms of overall efficiency of the drug. A non-destructive rapid near-infrared spectroscopy detection system for key chemical components and biological activity of Lanqin oral solution (LOS), one of the best-selling TCM formulations, was established for comprehensive quality evaluation. Near infrared spectral scanning was carried out on 101 batches of commercial LOS under the penetrated vial state and traditional state. RAW 264.7 cells were cultured to detect the anti-inflammatory ability of LOS, and the reference concentrations of epigoitrin, geniposide, and baicalin were obtained by HPLC. The quantitative models were optimized by three kinds of variable selection methods. The correlation coefficients of prediction value of the models were greater than 0.94. The system also passed the external validation. The performance of the non-invasive models was similar to the traditional models. The established non-destructive system can be applied to the rapid quality inspection of LOS to avoid unqualified drugs from entering the market and ensure drug effectiveness. The biological activity index of LOS was introduced and predicted by NIRs for the first time, which provides a new idea about the quality control of TCM formulations.
Proliferation and Invasion of Melanoma Are Suppressed by a Plant Protease Inhibitor, Leading to Downregulation of Survival/Death-Related Proteins
Authors: Bonturi Camila Ramalho , Salu Bruno Ramos , Bonazza Camila Nimri , Sinigaglia Rita de Cassia , Rodrigues Tiago , Alvarez-Flores Miryam Paola , Chudzinski-Tavassi Ana Marisa , Oliva Maria Luiza Vilela
Published: May 2022

Cell adhesion and migration are crucial for cancer progression and malignancy. Drugs available for the treatment of metastatic melanoma are expensive and unfit for certain patients. Therefore, there is still a need to identify new drugs that block tumor cell development. We investigated the effects of Enterolobium contortisiliquum trypsin inhibitor (EcTI), a protease inhibitor, on cell viability, cell migration, invasion, cell adhesion, and cell death (hallmarks of cancer) in vitro using human melanoma cells (SK-MEL-28 and CHL-1). Although EcTI did not affect non-tumor cells, it significantly inhibited the proliferation, migration, invasion, and adhesion of melanoma cells. Investigation of the underlying mechanisms revealed that EcTI triggered apoptosis and nuclear shrinkage, increased PI uptake, activated effector caspases-3/7, and produced reactive oxygen species (ROS). Furthermore, EcTI disrupted the mitochondrial membrane potential, altered calcium homeostasis, and modified proteins associated with survival and apoptosis/autophagy regulation. Acridine orange staining indicated acidic vesicular organelle formation upon EcTI treatment, demonstrating a cell death display. Electronic microscopy corroborated the apoptotic pattern by allowing the visualization of apoptotic bodies, mitochondrial cristae disorganization, and autophagic vesicles. Taken together, these results provide new insights into the anti-cancer properties of the natural EcTI protein, establishing it as a promising new therapeutic drug for use in melanoma treatment.
The Effect of Precipitation pH on Protein Recovery Yield and Emulsifying Properties in the Extraction of Protein from Cold-Pressed Rapeseed Press Cake
Authors: Ahlström Cecilia , Thuvander Johan , Rayner Marilyn , Matos María , Gutiérrez Gemma , Östbring Karolina
Published: May 2022

Rapeseed is the second most cultivated oilseed after soybean and is mainly used to produce vegetable oil. The by-product rapeseed press cake is rich in high-quality proteins, thus having the possibility of becoming a new plant protein food source. This study aimed to investigate how the precipitation pH affects the protein yield, protein content, and emulsifying properties when industrially cold-pressed rapeseed press cake is used as the starting material. Proteins were extracted under alkaline conditions (pH 10.5) with an extraction coefficient of 52 ± 2% followed by precipitation at various pH (3.0–6.5). The most preferred condition in terms of process efficiency was pH 4.0, which is reflected in the zeta potential results, where the proteins’ net charge was 0 at pH 4.2. pH 4.0 also exhibited the highest protein recovery yield (33 ± 0%) and the highest protein concentration (64 ± 1%, dry basis). Proteins precipitated at pH 6.0–6.5 stabilized emulsions with the smallest initial droplet size, although emulsions stabilized by rapeseed protein precipitated at pH 5.0–6.0 showed the highest emulsion stability at 37 °C for 21 days, with a limited layer of free oil. Overall, emulsion stabilized by protein precipitated at pH 5.0 was the most stable formulation, with no layer of free oil after 21 days of incubation.
Bile Acids Transporters of Enterohepatic Circulation for Targeted Drug Delivery
Authors: Durník Robin , Šindlerová Lenka , Babica Pavel , Jurček Ondřej
Published: May 2022

Bile acids (BAs) are important steroidal molecules with a rapidly growing span of applications across a variety of fields such as supramolecular chemistry, pharmacy, and biomedicine. This work provides a systematic review on their transport processes within the enterohepatic circulation and related processes. The focus is laid on the description of specific or less-specific BA transport proteins and their localization. Initially, the reader is provided with essential information about BAs′ properties, their systemic flow, metabolism, and functions. Later, the transport processes are described in detail and schematically illustrated, moving step by step from the liver via bile ducts to the gallbladder, small intestine, and colon; this description is accompanied by descriptions of major proteins known to be involved in BA transport. Spillage of BAs into systemic circulation and urine excretion are also discussed. Finally, the review also points out some of the less-studied areas of the enterohepatic circulation, which can be crucial for the development of BA-related drugs, prodrugs, and drug carrier systems.
Exploring the Extraction Methods of Phenolic Compounds in Daylily (Hemerocallis citrina Baroni) and Its Antioxidant Activity
Authors: Hao Zhilin , Liang Li , Liu He , Yan Yi , Zhang Yuyu
Published: May 2022

Daylily is a valuable plant resource with various health benefits. Its main bioactive components are phenolic compounds. In this work, four extraction methods, ultrasonic-assisted water extraction (UW), ultrasonic-assisted ethanol extraction (UE), enzymatic-assisted water extraction (EW), and enzymatic-assisted ethanol extraction (EE), were applied to extract phenolic compounds from daylily. Among the four extracts, the UE extract exhibited the highest total phenolic content (130.05 mg/100 g DW) and the best antioxidant activity. For the UE extract, the DPPH value was 7.75 mg Trolox/g DW, the FRAP value was 14.54 mg Trolox/g DW, and the ABTS value was 15.37 mg Trolox/g DW. A total of 26 phenolic compounds were identified from the four extracts, and the UE extract exhibited a higher abundance range of phenolic compounds than the other three extracts. After multivariate statistical analysis, six differential compounds were selected and quantified, and the UE extract exhibited the highest contents of all six differential compounds. The results provided theoretical support for the extraction of phenolic compounds from daylily and the application of daylily as a functional food.
A Calixarene Assembly Strategy of Combined Anti-Neuroinflammation and Drug Delivery Functions for Traumatic Brain Injury Therapy
Authors: Wang Chunxiao , Chang Yu-Xuan , Chen Xi , Bai Lihuan , Wang Heping , Pan Yu-Chen , Zhang Chunqiu , Guo Dong-Sheng , Xue Xue
Published: May 2022

Excessive inflammatory reaction aggravates brain injury and hinders the recovery of neural function in nervous system diseases. Microglia, as the major players of neuroinflammation, control the progress of the disease. There is an urgent need for effective non-invasive therapy to treat neuroinflammation mediated by microglia. However, the lack of specificity of anti-inflammatory agents and insufficient drug dose penetrating into the brain lesion area are the main problems. Here, we evaluated a series of calixarenes and found that among them the self-assembling architecture of amphiphilic sulfonatocalix[8]arene (SC8A12C) had the most potent ability to suppress neuroinflammation in vitro and in vivo. Moreover, SC8A12C assemblies were internalized into microglia through macropinocytosis. In addition, after applying the SC8A12C assemblies to the exposed brain tissue, we observed that SC8A12C assemblies penetrated into the brain parenchyma and eliminated the inflammatory factor storm, thereby restoring neurobiological functions in a mouse model of traumatic brain injury.
Structural and Magnetic Phase Transitions in BiFe1 − xMnxO3 Solid Solution Driven by Temperature
Authors: Karpinsky Dmitry V. , Silibin Maxim V. , Latushka Siarhei I. , Zhaludkevich Dmitry V. , Sikolenko Vadim V. , Al-Ghamdi Hanan , Almuqrin Aljawhara H. , Sayyed M. I. , Belik Alexei A.
Published: May 2022

The crystal structure and magnetic state of the (1 − x)BiFeO3-(x)BiMnO3 solid solution has been analyzed by X-ray diffraction using lab-based and synchrotron radiation facilities, magnetization measurements, differential thermal analysis, and differential scanning calorimetry. Dopant concentration increases lead to the room-temperature structural transitions from the polar-active rhombohedral phase to the antipolar orthorhombic phase, and then to the monoclinic phase accompanied by the formation of two-phase regions consisting of the adjacent structural phases in the concentration ranges 0.25 < x1 < 0.30 and 0.50 ≤ x2 < 0.65, respectively. The accompanied changes in the magnetic structure refer to the magnetic transitions from the modulated antiferromagnetic structure to the non-colinear antiferromagnetic structure, and then to the orbitally ordered ferromagnetic structure. The compounds with a two-phase structural state at room temperature are characterized by irreversible temperature-driven structural transitions, which favor the stabilization of high-temperature structural phases. The magnetic structure of the compounds also exhibits an irreversible temperature-induced transition, resulting in an increase of the contribution from the magnetic phase associated with the high-temperature structural phase. The relationship between the structural parameters and the magnetic state of the compounds with a metastable structure is studied and discussed depending on the chemical composition and heating prehistory.
Effect of Thermal Radiation on Three-Dimensional Magnetized Rotating Flow of a Hybrid Nanofluid
Authors: Asghar Adnan , Lund Liaquat Ali , Shah Zahir , Vrinceanu Narcisa , Deebani Wejdan , Shutaywi Meshal
Published: May 2022

The effect of thermal radiation on the three-dimensional magnetized rotating flow of a hybrid nanofluid has been numerically investigated. Enhancing heat transmission is a contemporary engineering challenge in a range of sectors, including heat exchangers, electronics, chemical and biological reactors, and medical detectors. The main goal of the current study is to investigate the effect of magnetic parameter, solid volume fraction of copper, Eckert number, and radiation parameter on velocity and temperature distributions, and the consequence of solid volume fraction on declined skin friction and heat transfer against suction and a stretching/shrinking surface. A hybrid nanofluid is a contemporary type of nanofluid that is used to increase heat transfer performance. A linear similarity variable is–applied to convert the governing partial differential equations (PDEs) into corresponding ordinary differential equations (ODEs). Using the three-stage Labatto III-A method included in the MATLAB software’s bvp4c solver, the ODE system is solved numerically. In certain ranges of involved parameters, two solutions are received. The temperature profile θη upsurges in both solutions with growing values of EC and Rd. Moreover, the conclusion is that solution duality exists when the suction parameter S≥Sci, while no flow of fluid is possible when S<Sci. Finally, stability analysis has been performed and it has been found that only the first solution is the stable one between both solutions.
The Non-Coding RNA Journal Club: Highlights on Recent Papers—11
Authors: Bonnet Hélène , Bogard Baptiste , Hubé Florent , Ilieva Mirolyuba , Uchida Shziuka , Ariza-Mateos Maria , Serganov Alexander , Pardini Barbara , Naccarati Alessio , Santulli Gaetano , Varzideh Fahimeh , Xiao Hua , Shiu Patrick
Published: May 2022

We are delighted to share with you our eleventh Journal Club and highlight some of the most interesting papers published recently [...]
Simulating Increased Permafrost Peatland Plant Productivity in Response to Belowground Fertilisation Using the JULES Land Surface Model
Authors: Vitali Rayanne , Chadburn Sarah E. , Keuper Frida , Harper Anna B. , Burke Eleanor J.
Published: May 2022

Several experimental studies have shown that climate-warming-induced permafrost thaw releases previously unavailable nitrogen which can lower nitrogen limitation, increase plant productivity, and counteract some of the carbon released from thawing permafrost. The net effect of this belowground fertilisation effect remains debated and is yet to be included in Earth System models. Here, we included the impact of thaw-related nitrogen fertilisation on vegetation in the Joint UK Land Environment Simulator (JULES) land surface model for the first time. We evaluated its ability to replicate a three-year belowground fertilisation experiment in which JULES was generally able to simulate belowground fertilisation in accordance with the observations. We also ran simulations under future climate to investigate how belowground nitrogen fertilisation affects the carbon cycle. These simulations indicate an increase in plant-available inorganic nitrogen at the thaw front by the end of the century with only the productivity of deep-rooting plants increasing in response. This suggests that deep-rooting species will have a competitive advantage under future climate warming. Our results also illustrate the capacity to simulate belowground nitrogen fertilisation at the thaw front in a global land surface model, leading towards a more complete representation of coupled carbon and nitrogen dynamics in the northern high latitudes.
Development and Validation of a Food and Nutrition Literacy Questionnaire for Chinese Adults
Authors: Zhang Yaqin , Zhang Zhaofeng , Xu Meihong , Aihemaitijiang Sumiya , Ye Chen , Zhu Wenli , Ma Guansheng
Published: May 2022

The purpose of this study aimed to develop and validate the Food and Nutrition Literacy Questionnaire for Chinese adults (FNLQ). The dimensions and core components of Food and Nutrition Literacy were constructed though literature review and qualitative consensus study. A cross-sectional survey of 8510 participants was conducted. The reliability of the questionnaire was determined by internal consistency, the construct validity was assessed by exploratory factor analysis (EFA) and confirmatory factor analysis (CFA), and the content validity was assessed by the Pearson correlation coefficient. From the literature review and qualitative methods, 20 core components and 50 questions of the FNLQ were developed, including 1 dimension of knowledge and 3 practice dimensions (ability of selection, preparing food and eating). The overall FNLQ questionnaire had good reliability and validity (Cronbach’s α = 0.893, χ2/DF = 4.750, RMSEA = 0.048, GFI = 0.891 and AGFI = 0.876). The average FNLQ score of all participants was (64.08 ± 12.77), and the score for the knowledge and understanding dimension was higher than that for the practice dimensions. In addition, 80 was set as the nutritional literacy threshold, and only 12.2% met this threshold in this survey. Sociodemographic and health status characteristics were predictors of FNLQ (R2 = 0.287, F = 244.132, p < 0.01). In conclusion, the FNLQ built in this study had good validity and reliability. It could be considered as a reliable tool to assess Food and Nutrition Literacy of Chinese adults.
Maternal Underweight and Its Association with Composite Index of Anthropometric Failure among Children under Two Years of Age with Diarrhea in Bangladesh
Authors: Das Rina , Kabir Md Farhad , Ashorn Per , Simon Jonathon , Chisti Mohammod Jobayer , Ahmed Tahmeed
Published: May 2022

Malnutrition in women has been a long-standing public health concern, with serious effects on child survival and development. Maternal body mass index (BMI) is an important maternal nutritional indicator. There are few published studies although child anthropometric failures do not occur in isolation and identifying children with single versus several co-occurring failures can better capture cases of growth failure in combination: stunting, wasting, and underweight. In the context of multiple anthropometric failures, traditional markers used to assess children’s nutritional status tend to underestimate overall undernutrition. Using the composite index of anthropometric failure (CIAF), we aimed to assess the association between maternal undernutrition and child undernutrition among children with diarrhea under the age of two and to investigate the correlates. Using 1431 mother-child dyads from the Antibiotic for Children with Diarrhea (ABCD) trial, we extracted children’s data at enrollment and on day 90 and day 180 follow-ups. ABCD was a randomized, multi-country, multi-site, double-blind, placebo-controlled clinical trial. The Bangladesh site collected data from July 2017 to July 2019. The outcome variable, CIAF, allows combinations of height-for-age, height-for-weight, and weight-for-age to determine the overall prevalence of undernutrition. The generalized estimating equation was used to explore the correlates of CIAF. After adjusting all the potential covariates, maternal undernutrition status was found to be strongly associated with child undernutrition using the CIAF [aOR: 1.4 (95% CI: 1.0, 1.9), p-value = 0.043] among the children with diarrhea under 2 years old. Maternal higher education had a protective effect on CIAF [aOR: 0.7 (95% CI: 0.5, 0.9), p-value = 0.033]. Our study findings highlight the importance of an integrated approach focusing on maternal nutrition and maternal education could affect a reduction in child undernutrition based on CIAF.
Persistent Properties of a Subpopulation of Cancer Cells Overexpressing the Hedgehog Receptor Patched
Authors: Feliz Morel Álvaro Javier , Hasanovic Anida , Morin Aurélie , Prunier Chloé , Magnone Virginie , Lebrigand Kevin , Aouad Amaury , Cogoluegnes Sarah , Favier Judith , Pasquier Claude , Mus-Veteau Isabelle
Published: May 2022

Despite the development of new therapeutic strategies, cancer remains one of the leading causes of mortality worldwide. One of the current major challenges is the resistance of cancers to chemotherapy treatments inducing metastases and relapse of the tumor. The Hedgehog receptor Patched (Ptch1) is overexpressed in many types of cancers. We showed that Ptch1 contributes to the efflux of doxorubicin and plays an important role in the resistance to chemotherapy in adrenocortical carcinoma (ACC), a rare cancer which presents strong resistance to the standard of care chemotherapy treatment. In the present study, we isolated and characterized a subpopulation of the ACC cell line H295R in which Ptch1 is overexpressed and more present at the cell surface. This cell subpopulation is more resistant to doxorubicin, grows as spheroids, and has a greater capability of clonogenicity, migration, and invasion than the parental cells. Xenograft experiments performed in mice and in ovo showed that this cell subpopulation is more tumorigenic and metastatic than the parental cells. These results suggest that this cell subpopulation has cancer stem-like or persistent cell properties which were strengthened by RNA-seq. If present in tumors from ACC patients, these cells could be responsible for therapy resistance, relapse, and metastases.
Laser Synthesized Core-Satellite Fe-Au Nanoparticles for Multimodal In Vivo Imaging and In Vitro Photothermal Therapy
Authors: Griaznova Olga Yu. , Belyaev Iaroslav B. , Sogomonyan Anna S. , Zelepukin Ivan V. , Tikhonowski Gleb V. , Popov Anton A. , Komlev Aleksei S. , Nikitin Petr I. , Gorin Dmitry A. , Kabashin Andrei V. , Deyev Sergey M.
Published: May 2022

Hybrid multimodal nanoparticles, applicable simultaneously to the noninvasive imaging and therapeutic treatment, are highly demanded for clinical use. Here, Fe-Au core-satellite nanoparticles prepared by the method of pulsed laser ablation in liquids were evaluated as dual magnetic resonance imaging (MRI) and computed tomography (CT) contrast agents and as sensitizers for laser-induced hyperthermia of cancer cells. The biocompatibility of Fe-Au nanoparticles was improved by coating with polyacrylic acid, which provided excellent colloidal stability of nanoparticles with highly negative ζ-potential in water (−38 ± 7 mV) and retained hydrodynamic size (88 ± 20 nm) in a physiological environment. The ferromagnetic iron cores offered great contrast in MRI images with r2 = 11.8 ± 0.8 mM−1 s−1 (at 1 T), while Au satellites showed X-ray attenuation in CT. The intravenous injection of nanoparticles enabled clear tumor border visualization in mice. Plasmonic peak in the Fe-Au hybrids had a tail in the near-infrared region (NIR), allowing them to cause hyperthermia under 808 nm laser exposure. Under NIR irradiation Fe-Au particles provided 24.1 °C/W heating and an IC50 value below 32 µg/mL for three different cancer cell lines. Taken together, these results show that laser synthesized Fe-Au core-satellite nanoparticles are excellent theranostic agents with multimodal imaging and photothermal capabilities.
Triterpene Content in Flesh and Peel of Apples Grown on Different Rootstocks
Authors: Butkevičiūtė Aurita , Janulis Valdimaras , Kviklys Darius
Published: May 2022

Advancements in rootstock breeding and selection have revolutionized the manner in which apples are grown throughout the world. Fruit tree breeding has typically focused on key horticultural characteristics. Even though agents with health benefits have been investigated more frequently during the recent years, information about the effect of different cultivation factors, such as the rootstock, on triterpene concentration is still lacking. The present study aimed to evaluate triterpene profiles and the quantitative composition of different parts of apple fruit that was grown on 17 various origin and vigor rootstocks. HPLC analyses of triterpenes in apple samples were performed. The highest total content of triterpenes (7.72 ± 0.39 mg/g) was found in peel samples of apples grown on the dwarf rootstock 62-396-B10®. Depending on the rootstock, apple peel samples accumulated 3.52 to 4.74 times more triterpene compounds than apple flesh samples. Ursolic acid was the predominant triterpene compound in apple peel and flesh samples. The highest content of ursolic acid (5.84 ± 0.29 mg/g) was found in peel samples of apples grown on the dwarf rootstock 62-396-B10®. Meanwhile, the lowest amount of ursolic acid (3.25 ± 0.16 mg/g) was found in apple peel samples grown on the dwarf rootstock Cepiland-Pajam®2. A proper match of a cultivar and a rootstock can program a fruit tree to grow larger amounts of higher quality, antioxidant-rich, and high-nutrition-value fruit.
Effect of Irrigation Dose on Powdery Mildew Incidence and Root Biomass of Sessile Oaks (Quercus petraea (Matt.) Liebl.)
Authors: Kasprzyk Winicjusz , Baranowska Marlena , Korzeniewicz Robert , Behnke-Borowczyk Jolanta , Kowalkowski Wojciech
Published: May 2022

The sessile oak is one of the most significant forest tree species in Europe. This species is vulnerable to various stresses, among which drought and powdery mildew have been the most serious threats. The aim of this study was to determine the influence of irrigation levels (overhead sprinklers) on the damage caused by powdery mildew to Quercus petraea growing in a nursery setting. Four irrigation rates were used: 100%, 75%, 50% and 25% of the full rate. The area of the leaves was measured and the ratio between the dry mass of the roots and the dry mass of the entire plant was calculated after the growing season in years’ 2015 and 2016. Limiting the total amount of water provided to a level between 53.6 mm × m−2 and 83.6 mm × m−2, particularly in the months when total precipitation was low (VII and VIII 2015), a supplemental irrigation rate between 3 and 9 mm × m−2 resulted in a lower severity of oak powdery mildew on leaves and lead to a favorable allocation of the biomass of the sessile oak seedlings to the root system. The severity of infection on oak leaf blades was lower when irrigation rates were reduced. The greatest mean degree of infestation in 2015 was noted in the 100% irrigation rate (14.6%), 75% (6.25%), 50% (4.35%) and 25% (5.47%). In 2016, there was no significant difference between the mean area of leaves infected by powdery mildew depending on the applied irrigation rate. The shoot-root biomass rate showed greater variation under limited irrigation rates. Controlling the irrigation rate can become an effective component of integrated protection strategies against this pathogen.
Cyano-Phycocyanin: Mechanisms of Action on Human Skin and Future Perspectives in Medicine
Authors: Dranseikienė Daiva , Balčiūnaitė-Murzienė Gabrielė , Karosienė Jūratė , Morudov Dmitrij , Juodžiukynienė Nomeda , Hudz Nataliia , Gerbutavičienė Rima Jūratė , Savickienė Nijolė
Published: May 2022

Cyano-phycocyanin is one of the active pigments of the blue-green algae and is usually isolated from the filamentous cyanobacteria Arthrospira platensis Gomont (Spirulina). Due to its multiple physiological functions and non-toxicity, cyano-phycocyanin may be a potential substance for the topical treatment of various skin diseases. Considering that the conventional medicine faces drug resistance, insufficient efficacy and side effects, the plant origin compounds can act as an alternative option. Thus, the aim of this paper was to review the wound healing, antimicrobial, antioxidative, anti-inflammatory, antimelanogenic and anticancer properties and mechanisms of cyano-phycocyanin topical activities on human skin. Moreover, possible applications and biotechnological requirements for pharmaceutical forms of cyano-phycocyanin for the treatment of various skin diseases are discussed in this review.
Characterization of Triterpene Saponin Glycyrrhizin Transport by Glycyrrhiza glabra
Authors: Kato Kakuki , Horiba Asako , Hayashi Hiroaki , Mizukami Hajime , Terasaka Kazuyoshi
Published: May 2022

Glycyrrhizin (GL), a triterpene compound produced by Glycyrrhiza species, is a crucial pharmacologically active component of crude drugs. In contrast to the biosynthesis of GL in plants, little is known about GL transport and accumulation in plants. The transport mechanism of GL was characterized using cultured cells of Glycyrrhiza glabra. Cultured cells of G. glabra efficiently incorporated exogenously supplied GL. Proton pump inhibitors, such as probenecid and niflumic acid, as well as a protonophore (carbonylcyanide m-chlorophenylhydrazone), markedly inhibited GL uptake by cultured cells, whereas vanadate exhibited a moderate inhibition. Furthermore, GL transport by G. glabra tonoplast vesicles is dependent not on a H+-electrochemical gradient but MgATP and is markedly inhibited by vanadate. These results suggest that GL uptake by cultured cells is mediated by a H+-symporter in the plasma membrane and an ATP-binding cassette transporter, which has high specificity for the aglycone structure of GL on the tonoplast.
Exploration of Soil Functional Microbiomes—A Concept Proposal for Long-Term Fertilized Grasslands
Authors: Stoian Vlad , Vidican Roxana , Florin Păcurar , Corcoz Larisa , Pop-Moldovan Victoria , Vaida Ioana , Vâtcă Sorin-Daniel , Stoian Valentina Ancuța , Pleșa Anca
Published: May 2022

Exploring grassland microbiomes is a challenge in the current context of linking soil microorganism activity with the balance of these ecosystems. Microbiologists are constantly attempting to develop faster and lower-cost methods, and propose new and best-fitted indicators that will provide a more complex data analysis. A different concept was proposed for assessing functional microbiomes by splitting the functional ecological niche into complementary segments. The comparison with the upper and lower limits of the ecological niche provides a clearer image of community alterations due to long-term applied treatments. The method allows the extraction of the most sensitive and stable functional guilds, with the extraction of the most critical dominant–codominant functional groups in every segment of the functional niche. The resulting microbial functional–sociological model is ready to use on community-level physiological profile databases and also can be applied backward for vegetation analysis.
The Effect of Two Different Light-Curing Units and Curing Times on Bulk-Fill Restorative Materials
Authors: Bayrak Gokcen Deniz , Yaman-Dosdogru Elif , Selvi-Kuvvetli Senem
Published: May 2022

This study aimed to evaluate the effect of two different light-curing units and curing times on the surface microhardness (SMH), compressive strength (CS), and volumetric shrinkage (VS) of four restorative materials (FiltekTM Z250, FiltekTM Bulk Fill Posterior, Beautifil® Bulk Restorative, ACTIVATM BioACTIVE). For all tests, each material was divided into two groups depending on the curing unit (Woodpecker LED-E and CarboLED), and each curing unit group was further divided into two subgroups according to curing time (10 s and 20 s). SMH was evaluated using a Vickers hardness tester, CS was tested using a universal testing machine, and VS was measured using video imaging. In all the restorative materials cured with Woodpecker LED-E, the 20 s subgroup demonstrated significantly higher SMH values than the 10 s subgroup. In both light-curing time subgroups, the CarboLED group showed significantly higher CS values than the Woodpecker LED-E group for all restorative materials except FiltekTM Bulk Fill Posterior cured for 20 s. ACTIVATM BioACTIVE showed significantly greater volumetric change than the other restorative materials. A higher curing light intensity and longer curing time had a positive effect on the SMH and CS of the restorative materials tested in this study. On the other hand, curing unit and time did not show a significant effect on the VS values of restorative materials.
Aging Characteristics of Plant Hot-Mix Recycled Asphalt and Its Induced Aging on New Asphalt
Authors: Chen Huiqiang , Tang Ge , Wang Xiong
Published: May 2022

In order to explore the aging process and mechanism of new and old asphalt in plant hot-mix recycled asphalt mixture during the mixing and use process, the liquid viscosity test and low-temperature bending creep test are carried out to test the dynamic viscosity, stiffness modulus, creep rate, and low-temperature flexibility of the matrix asphalt, recycled asphalt, and old and new asphalt after rolling thin film oven test (RTFOT) aging and pressurized aging vessel (PAV) aging. The macroscopic performance attenuation law of new and old asphalt during the aging process in thermal regeneration is compared and analyzed. After that, the aging process and mechanism of new and old asphalt are explored by infrared spectroscopy and differential calorimetric analysis scanning. The results show that RTFOT aging and PAV aging make the viscosity of recycled asphalt rise significantly and the low temperature performance decline rapidly. After RTFOT aging and PAV aging of new and old asphalt, the dynamic viscosity and low-temperature performance change range is much higher than that of the matrix asphalt. Some technical indicators are even closer to the recycled asphalt after aging, which proves that its aging speed is faster than that of the matrix asphalt. Meanwhile, the results of infrared spectroscopy and differential scanning calorimetry analysis show that in addition to the independent aging of new asphalt and recycled asphalt, there is also a chemical effect between them–that is, some active groups in recycled asphalt have a more obvious promotion effect on the aging process of new asphalt, here called “induced aging”. This induced aging changes the aging mechanism of the matrix asphalt by changing the aging process of it, which greatly limits the popularization and application of thermal regeneration technology.
Grid-Based Employee Safety Behavior Risk Assessment of the Train Operation Department
Authors: Zhang Huafeng , Qi Changmao , Ma Mingyuan
Published: May 2022

In the train operation department, the most important and dynamic factor is that the department employees are involved in all areas. Realizing the dynamic control of “key person, key event, and key period” to fundamentally curb employee inertia violation is a significant issue that needs to be solved on the railway site. The traditional “probability–severity” two-dimensional risk assessment model is carried out from the perspective of the system, ignoring the spatiotemporal risk characteristics of the individual, and a large amount of hazard factor data generated in the operation process is not applied in the risk assessment process. As a result, safety behavior risk practice lacks pertinence, accuracy, and individuation. This study proposes a safety behavior risk assessment model based on the grid management and hazard factor assignment function to improve the traditional two-dimensional risk matrix. By introducing spatial location variables, the method accurately locates and classifies the site staff and organizes the disorder and lack of associated risk data with regard to time and space. With a focus on the hazard factor, the induced intensity is proposed for the first time and considered as the input of probability calculation to innovate the traditional “probability–severity” risk matrix. Finally, the methodology is applied to the risk event assessment of “the assistant watchman doesn’t appear as required” scenario in the Huangyangcheng station of Shenshuo Railway, and the evaluation results realize the personalized evaluation of the risk event in different cell grids.
The Visbreaking of Heavy Oil in Supercritical Cyclohexane: The Effect of H-Donation
Authors: Ding Lei , Wang Lin , Yang Jingyi , Yuan Peiqing , Huang Zibin
Published: May 2022

The performance of heavy oil visbreaking in supercritical cyclohexane (SCC6H12) was evaluated, followed by a comparison with its reaction in supercritical benzene (SCC6H6). The dealkylation-based viscosity reduction in the SCC6H12 was accelerated by improving the diffusivity, through which a product viscosity (80 °C) as low as 0.5 Pa.s was readily obtained by visbreaking at 380 °C for 5 min. A competition between dealkylation and condensation took place throughout the visbreaking process. As the reaction proceeded or the temperature increased, condensation played an increasingly dominant role in the visbreaking. Unlike the inert SCC6H6, the SCC6H12 participated in the visbreaking by saturating the alkyl carbon radicals essential for dealkylation and the aromatic carbon radicals essential for condensation. The viscosity reduction efficiency of the visbreaking in the SCC6H12 was initially suppressed by the H-donation of the solvent, but recovered rapidly due to the improved diffusion environment. Benefiting from the saturation of the aromatic carbon radicals, the asphaltene content of the product obtained in the SCC6H12 was lower than the corresponding value of the product obtained in the SCC6H6.
Automatic Control System for Cane Honey Factories in Developing Country Conditions
Authors: Mejía Víctor Cerda , Mejía Galo Cerda , Rubio Octavio Guijarro , Cortes Isnel Benítez , Yordi Estela Guardado , Tenezaca Bernabe Ortega , Miño Valdés Juan , Suárez Erenio González , Martínez Amaury Pérez
Published: May 2022

(1) Background: A proposal for the automatic control of sugar cane honey factories based on simulation with real data is presented. (2) Methods: The P&ID diagram of the artisanal process is designed, as well as the measurement and control systems of the different process variables. A data acquisition and monitoring system is proposed with all the required equipment. Using GNU Octave software, the process was simulated, where the transfer functions and parameters of the different stages were determined. The transient responses of these systems are determined before step-jump type disturbances, as well as that of the controllers. (3) Results: A correct adjustment of the controllers is obtained, indicating those that work in a stable way before disturbance variations in the real ranges of plant work. (4) Conclusions: Simulation of controllers before different forcing functions in the ranges of the operating parameters allowed for establishing dynamic responses of each one, demonstrating that they are capable of adjusting the value of the variable of interest or the control, and determining control of the main operating variables.
Study of a Hydrophilic Healing-Promoting Porcine Acellular Dermal Matrix
Authors: Ding Zhuang , Dan Nianhua , Chen Yining
Published: May 2022

Sodium hyaluronate (SH) is recognized as the strongest natural humectant, since it contains a large number of hydroxyl and carboxyl groups in its structure, and can absorb 1000 times its own weight of water. The porcine acellular dermal matrix (pADM) has been widely used in biological materials for its biological activities, such as promoting cell proliferation and promoting wound healing. Enhancing the hydrophilic and moisturizing properties of the pADM is expected to further improve its ability to promote wound healing. However, there are no strong chemical bonds between SH and pADM. Therefore, SH was oxidized by sodium periodate in this study, and was further used to cross-link it with pADM. The microstructure, hydrophilicity, moisture retention, degradation and cytotoxicity of pADM cross-linked with different oxidation degrees of oxidized sodium hyaluronate (OSH) were studied. The results show that OSH-pADM maintained the secondary structure of natural collagen, as well as the good microporous structure of native pADM after cross-linking. With increasing oxidation degree, the surface hydrophilicity and moisture retention capacities of OSH-pADM increased; among them, OSH-pADM cross-linked with 40% oxidation degree of OSH was found to have the strongest moisture retention capacity. The hygroscopic kinetics at 93% RH were conformed to the second-order hygroscopic kinetics equation, indicating that the hygroscopic process was controlled by chemical factors. The degradation resistance of OSH-pADM also increased with increasing oxidation degree, and the cytotoxicity of OSH-pADM was acceptable. The in vivo full-thickness wound healing experiments showed that OSH-pADM had an obvious ability to promote wound healing. It can be speculated that OSH-pADM, with its good hydrophilic and moisturizing properties, physicochemical properties and biocompatibility, has great potential for facilitating wound repair.
Societal Evaluation of Bioeconomy Scenarios for Germany
Authors: Zander Katrin , Will Sabine , Göpel Jan , Jung Christopher , Schaldach Rüdiger
Published: May 2022

Transitioning to a bio-based economy is widely perceived as a necessary way to tackle climate change and other key environmental challenges. Given the major socio-economic consequences that such a transition entails for people’s everyday lives, it is crucial to take account of citizens’ perceptions and levels of acceptance of these changes. This study applies a holistic approach to gain an understanding of how citizens in Germany assess possible developments associated with transitioning to a bioeconomy. We developed three future scenarios modelling the impacts on people’s day-to-day lives of adopting different elements of a bioeconomy and of replacing fossil resources with biogenic resources to a lesser or greater extent by 2050. German citizens were asked to evaluate the different scenarios through a quantitative online survey. Although the respondents largely preferred the scenario of “Bioeconomy Change” entailing the most substantial changes in resource use, many also expressed concerns about the possible negative socio-economic effects of this scenario, including fears of increased product prices, declining living standards, and greater social injustice. The results show that younger people and women are more in favour of changes towards a bioeconomy than men and older people. Since the acceptance of bio-technical innovations increases with greater knowledge about such innovations and their usefulness, the transformation process must make use of sound communication strategies that involve all societal groups by engaging them in constructive dialogue.
Is the Financial Report Quality Important in the Default Prediction? SME Portuguese Construction Sector Evidence
Authors: Costa Magali , Lisboa Inês , Gameiro Ana
Published: May 2022

This work analyses whether financial information quality is relevant to explaining firms’ probability of default. A financial default prediction model for SMEs (Small and Medium Enterprises) is presented, which includes not only traditional measures but also financial reporting quality (FRQ) measures. FRQ influences the decision-making due to its impact on financial information, which has repercussions on the accounting ratios’ informativeness. A panel data of 1560 Portuguese SMEs in the construction sector, from 2012 to 2018, is analysed. First, firms are classified as default or compliant using an ex-ante criterion which allows us to identify signs of financial constraints in advance. Then, the stepwise method is employed to identify which variables are more relevant to explain the default probability. Results show that FRQ measures, namely accruals quality and timeliness, impact firms’ defaulting, supporting their relevance in predicting financial difficulties. Finally, using a logit approach, the accuracy of the model increased when FRQ variables were included. Results are confirmed using “new age” classifiers, namely the random forest methodology. This work is not only relevant to the extant financial distress literature but has also relevant implications for practice since stakeholders can understand the impact of financial reporting quality to prevent additional risks.