bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of ENSO on the intraseasonal oscillations of sea surface temperature and wind speed along Vietnam’s coastal areas
Authors:   Vietnam Institute of Meteorology, Hydrology and Climate Change, Le Quoc Huy , Tran Thuc ,   Vietnam Institute of Meteorology, Hydrology and Climate Change, Nguyen Xuan Hien ,   Vietnam Institute of Meteorology, Hydrology and Climate Change, Dinh Van Uu ,   University of Science, Vietnam National University, Hanoi
Published: June 2018

Our study applied the Ensemble Empirical Mode Decomposition (EEMD) method to analyze intraseasonal variability (ISV) of sea surface temperature (SST) and wind speed using a 22-year monitoring data set from 10 coastal stations. Results show that the El Niño and Southern Oscillation(ENSO) significantly affected the ISO Quasi-Biennial Oscillation (QBWO) 10-20 day periods and Madden-Julian Oscillation (MJO) 30-60 day periods of SST and wind speed at the coastal stations. As seen with MJO, the effects of ENSO on SST tend to increase from the north to south, whereas its impact on wind speed decreases from the north to south of Vietnam’s coastal areas. In contrast, with QBWO, the effect of ENSO on SST reduces moving from the north to south, whereas its impact on wind speed increases from the north to south of Vietnam’s coastal areas.
Development of a methodological framework for calculation of carbon footprint of rice production in Vietnam
Authors:   Vietnam Institute of Meteorology, Hydrology and Climate Change, Dao Minh Trang , Huynh Thi Lan Huong ,   Vietnam Institute of Meteorology, Hydrology and Climate Change
Published: June 2018

Currently, there are various standards and guidelines to calculate product carbon footprints in the world such as the Greenhouse Gas (GHG) Protocol of the World Resources Institute/World Business Council for Sustainable Development (WRI/WBCSD), ISO 14067, and PAS 2050. Most of the studies on carbon footprints of rice production adopt the ISO Life Cycle Assessment (LCA) method while very few studies apply PAS 2050 and the Greenhouse Gas Protocol Agricultural Guidance of WRI/WBCSD. However, the above standards and guidelines do not provide a separate methodology for calculating carbon footprints of rice production. From that perspective, this research paper has developed a methodology to calculate the carbon footprints of rice production from the upstream processes, rice production process to post-farm stage. However, several sources of GHG emissions during the life cycle of rice have not been included in this methodological framework due to either the lack of data or complicated calculation methods.
QTL analysis on rice genotypes adapted to acid sulfate soils in the Mekong river delta, Vietnam
Authors:   Institute of Agricultural Sciences for Southern Vietnam, Bui Chi Buu , Nguyen Thi Lang ,   Cuu Long Delta Rice Research Institute
Published: June 2018

Three target points in acid sulfate soils have been identified as: 1) Aluminum (Al) toxicity; 2) Iron (Fe) toxicity; 3) Phosphorous (P)deficiency; and 4) Droughts at the seedling stage. The exploitation of gene pools from wild rice species fruitfully obtained a true introgression of desirable traits into high yielding varieties (HYVs), such as AS996 (IR64/Oryza rufipogon), which are tolerant to Al-toxicity, have short durations, high yields, and adaptability to acid sulfate soils. Major QTLs on chromosome 3 were detected to control Al-toxicity as identified through an analysis of the RIL population of IR64/O. rufipogon on control relative root length (RRL). RM232 was considered as a good marker linked to the target quantitative trait locus(QTL)on chromosome 3, then SR28 and OSR29 on chromosome 9 were also used.QTL mapping by 126 SSRs through 225 individuals of the F6 RILs population of AS996/OM2395 was carried out to find the P-uptake gene on chromosome 12. The promising genotype of OM4498 from the BC population of IR64/OMCS2000 was selected through MAS with RM235 and RM247 on chromosome 12 linked to QTL, which controls the P-deficiency tolerance. Based on the leaf bronzing index (LBI), SSR markers were used to select promising genotypes tolerant to iron-toxicity, such as RM315 and RM212 on chromosome 1, and RM252 and RM211 on chromosome 2. The intervals among RM315-RM212 on chromosome 1, RM6-RM240 on chromosome 2, and RM252-RM451 on chromosome 4, were continually studied through further fine mapping.A backcrossing mapping population that included 217 individuals of BC2F2, was set up from OM1490/WAB880-1-38-18-20-P1-HB to detect the QTLs relating to drought tolerance (DT). The QTL was located in the intervals between RM201-RM511 on chromosome 9. BAC clones 13A9 and 7O3 were used as pinpoints on the high solution map for new markers designed from their sequences. The markers became useful to help rice breeders possibly select the improved genotypes adapting to drought stress in the seedling stage.
Establishment of an in-house one-step real-time RT-PCR assay for detection of Zaire ebolavirus
Authors:   Vietnam Military Medical University, Ministry of Defense, Hoang Xuan Su , Dinh Thi Thu Hang ,   Vietnam Military Medical University, Ministry of Defense, Hoang Van Tong ,   Vietnam Military Medical University, Ministry of Defense, Ho Huu Tho ,   Vietnam Military Medical University, Ministry of Defense, Bui Tien Sy ,   108 Military Central Hospital, Ministry of Defense, Nguyen Van An ,   Vietnam Military Medical University, Ministry of Defense, Nguyen Thai Son ,   Vietnam Military Medical University, Ministry of Defense
Published: June 2018

Ebola virus is a deadly causative agent with a high mortality rate of up to 90%, therefore it has been classified by the Center for Disease Control and Prevention (CDC) as a category A biological agent. The World Health Organization (WHO) recommended using RT-PCR based assays to rapidly detect the virus. In the present study, we established an in-house assay for detection of Zaire ebolavirus via real-time RT-PCR. The nucleotide sequence of the Zaire ebolavirus nucleoprotein (NP) gene was retrieved from the Genbank for designing primer pairs and probes using Primer Express 3.0 software. The RNA positive control was generated by in vitro RNA transcript synthesis. The optimal components in the 20 μl final volume of the real-time RT-PCR assay were 10 μl 2X QuantiTectProbeRT-PCR master mix, 0,6 μM of each primer, 0,1 μM of the probe, 0,2 μl RT mix and 5 μl of RNA template. The thermal cycle conditions were as follows: 50oC for 30 min, 95°C for 15 min, then 45 cycles of 15 s at 94°C, 60s at 60°C. The limit of detection of the assay was 100 copies/reaction and 1414 FFU/ml with the positive RNA panel and sample panel of RNA extracted from cell culture supernatants of cells infected with Zaire ebolavirus 2014/Gueckedou-C05, respectively. The specificity of this assay was 100% when tested with the positive RNA panel of Ebola virus and other haemorrhagic fever viruses. In conclusion, we successfully established an in-house real-time RT-PCR assay for detection of Zaire ebolavirus in Vietnam with a limit of detection of 1414 FFU/ml and specificity of 100%.
Preparation and characterization of liposomes encapsulating Calophyllum inophyllum oil
Authors:   Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Phan Huu Trong , Tran Van Thanh ,   Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City
Published: June 2018

Callophyllum inophyllum oil, also known as Tamanu oil, is reported to treat a wide range of skin problems such as acne, eczema, psoriasis, herpes, hemorrhoids, and injuries caused due to wounds, among others. Liposomes, which are effective carriers for topical treatment of dermal diseases, could enhance the therapeutic efficiency of Tamanu oil. Therefore, the purpose of this study was to formulate and characterize liposomes loading Tamanu oil. Liposomes encapsulating Tamanu oil with different ratios of Phospholipon 90G and L-α-lecithin were prepared using the thin-film hydration technique. Liposomal formulations were characterized in terms of aspect, particle size, size distribution, zeta potential, and morphology by using light microscope and dynamic light scattering analysis (DLS). Furthermore, the best formulation was tested with the storage stability after 30 days, and Tamanu oil loaded in the liposomes was identified as dictated in Vietnamese pharmacopoeia. The data demonstrated that an average liposome diameter of 53 nm with a narrow polydispersity (0.289) was obtained at a Phospholipon 90G to L-α-lecithin molar ratio of 4:6, and a Tamanu oil to phospholipid mass ratio of 1:3 approximately. In addition, according to the DLS results, the particle size and the zeta potential were quite stable at 2-8oC during 30 days of storage. The study achieved the promising results for developing a novel formulation containing Tamanu oil, which may be valuable to treatment of skin diseases.
Evaluating the systematic position of Ehretia asperula Zoll. & Moritzi based on ITS1, matK and trnL-trnF DNA sequences
Authors:   Institute of Genome Research, Vietnam Academy of Science and Technology, Nguyen Thuy Linh , Pham Thi Hang ,   Institute of Genome Research, Vietnam Academy of Science and Technology, Do Van Truong ,   Vietnam National Museum of Nature, Vietnam Academy of Science and Technology, Huynh Thi Thu Hue ,   Institute of Genome Research, Vietnam Academy of Science and Technology
Published: June 2018

Ehretia asperula,popular as a medicinal herb that has potential on cancer treatment, has a limited research about its phylogeny relationships. With many innovative advancements in molecular biology, it is more easy and reliable to identify the taxonomic position of a species by molecular markers- DNA barcode. In this study, we used three different markers, ITS1, trnL-trnF, and matK, to evaluate E. asperula’s systematic position. Based on ITS1 sequence, E. asperula belongs to clade Ehretia I and is a close relative of E. resinosa. Moreover, ITS1 was suggested to be use as a suitable DNA barcode in order to identify E. asperula.
ON THE CONTROL OF THE HEAT CONDUCTION
Authors: Fayziev Yusuf Ergashivich
Published: June 2018

Mathematical models of thermo control processes in a rectangular plate are considered. In the model under consideration, the temperature inside a plate is controlled by heat exchange through one boundary while the other three are insulated. The control parameter is a function that satisfies certain integral equations. Sufficient conditions for achieving the given projection of the temperature at a fixed point on the plate and given average temperature are studied. ABSTRAK: Model matematik bagi proses kawalan suhu dalam bekas segi empat tepat telah dipilih. Melalui model ini, suhu bekas dikawal dengan menukar haba melalui salah satu sisi bekas, manakala tiga sisi lain telah ditebat. Parameter kawalan ini ialah fungsi, di mana ia sesuai dengan persamaan sesetengah integral. Keadaan sesuai bagi mencapai suhu tetap bekas seperti cadangan dan suhu purata yang diberikan turut dikaji.
PRESCRIPTION AND ADEQUACY OF HEMODIALYSIS IN DEPARTMENT OF NEPHROLOGY AND DIALYSIS GI "INSTITUTE OF NEPHROLOGY OF NAMS OF UKRAINE" FOR 2012 YEAR.
Authors: Kulyzkyi M. V. , Busygina J. S. , Stashevska N. V. , Bilenko A. V. , Surgko L. M.
Published: June 2018

Work of department of HD of GI "Institute of nephrology of NAMSU" is analyzed and compared to mean for Ukraine and some regions according National register of patients with chronic kidney diseases 2011.
FEATURES OF THE MICROBIAL SPECTRUM UROGENITAL SYSTEM OF WOMEN WITH RECURRENT PYELONEPHRITIS
Authors: Romanenko O. , Stepanova N. , Rudenko A. , Kruglikov V. , Lebid L. , Kolesnyk M.
Published: June 2018

The aim of our research was to identify the peculiarities of the microbial spectrum urogenital system of the women with recurrent pyelonephritis.

Materials and methods. We have conducted a comprehensive micro-biological examination different biological material (urine, vaginal swabs, scraping of urethral and cervical canal’s) of 175 women with recurrent pyelonephritis. The patients were divided in accordance with frequency of recurrent. The microbial spectrum urogenital system of 98 women with recurrent pyelonephritis (group 1) was compared with 77 women with sporadic pyelonephritis (up to 2 times per year) (group 2). The research included the process of identification of bacterial agents, mollicutes, chlamydia as well as yeasts.

Results. In the women with recurrent pyelonephritis had the significant increase of frequency of identification of E. faecalis in urine (p=0.03), in urethral canal (p=0.05), and vaginal scraping (p=0.02). 48 women from 1st group had bacterial mix-infection. At the same time, the women from comparative group had only in 25 (32.6%) cases (p=0.027). The direct associative relationship was found between existence of S. epidermidis in urethral canal, in cervical canal, in vagina and the frequency of recurrent pyelonephritis (ρ=0.2, р=0.009; ρ=0.23, р=0.004 and ρ=0.19, р=0.01 respectively); E. faecalis in the urethral canal (ρ=0.19, р=0.02); Candida albicans in the vagina (ρ=0.17, р=0.03). 79.6% examined patients were infected by mollicutes, viruses and chlamydia.

Conclusions. Consistent high level of urethral’s and vaginal’s bacterial weight of the women with recurrent pyelonephritis leads to the migration of causative agents into bladder and to activates formation of recurrent of the disease. Identified peculiarities of microbial spectrum prove the necessity mandatory micro-biological research not only urine, but urethra, cervical canal, and vagina.
AGE–RELATED ASPECTS AND FEATURES OF THE USE OF METHODS OF EXTRACORPORAL THERAPY FOR CHILDREN WITH ACUTE RENAL FAILURE
Authors: Sheyman B.S. , Babicheva O.V. , Voloshina N.A. , Mazur S.V. , Urin A.A.
Published: June 2018

Aim of work it was to conduct the analysis of frequency and testimonies to application of auxiliary technologies of экстракорпорального treatment of children with ARFof the different age–related groups.
EXPRESSION OF INTERCELLULAR ADHESION MOLECULE (ICAM-1) ON LYMPHOCYTES AND THE LEVELS OF PRO- AND ANTIINFLAMMATORY CYTOKINES IN PATIENTS WITH PYELONEPHRITIS
Authors: Gaysenyuk F.Z.
Published: June 2018

The aim of this work is to investigate the level of mononuclear cells, expressing intercellular adhesion molecule (CD54+), pro- (IL-1, TNF-a) and antiinflammatory (IL-4, TGF-в) blood cytokines in the patients with pyelonephritis PN, to determine the peculiarities in acute and chronic its course.

Materials and methods. The immuno-enzymic method ELISA and the corresponding test-systems (“Diaclone”, “DRG”) were used to study the levels of blood cytokines and immuno-fluorescent with “Coltage” (USA) – the level of CD54+-cells.

Results. It has been demonstrated that CD54+-lymphocytes, IL-1, TNF-a, IL-4 and TGF-ft in blood were increased in patients with acute and chronic pyelonephritis, compared with healthies. The number of lymphocytes expressing CD54 and the level of TGF-ft were higher in patients with chronic pyelonephritis.

Conclusion. The high level of cytokines and CD54+-lymphocytes in blood ofpatients confirm their important role in immunogenesis of pyelonephritis, the interrelation is especially interesting between CD54+-cells and TGF-ft in chronic process.
FEATURES TOPICAL DIAGNOSIS OF CHRONIC INFLAMMATORY DISEASES OF THE PELVIC ORGANS CAUSED ÇHLAMYDTA TRACHOMATIS
Authors: Shcherbak M.O.
Published: June 2018

To analyze the information content of the topical diagnostic C.trachomatis in a comparative perspective in view of possible lesions.

Materials and methods: In this paper the results ofan assessment of topical diagnosis in 142 women with Chlamydia infection HPID from different parts of the fence biomaterial (cervix, urethra, tonsils, colon, and conjunctiva of the eye) by PCR.

Results: The majority of women with HPID - C.trachomatis postulation were the study of the biological material by PCR from the cervices, at 62.0% of the urethra, at 38.7% of the rectum, at 37.3 from the tonsils 17.6 and % of the conjunctiva of the eye.

Conclusions: conducting clinical and microbiological analysis of Chlamydia infection HPID allowing for the topical diagnosis revealed informative clinical extended analysis of the study pathology.
THEORY AND PRACTICE OF EVIDENCE-BASED MEDICINE: NEPHROLOGY. TYPES OF STUDY AND EVIDENCE LEVELS
Authors: Kolesnyk N. A. , Fomina S. P. , Nepomnyashchy V. N.
Published: June 2018

The basic features of different clinical studies types and their hierarchy considering strength of the evidence were considered. The quality assessment examples of information in medicine and data presentation options to form of evidence were presented. The role of a clinician as a participant in evidence process was underlined.
SPECIFIC INFLAMMATORY PROTEINS IN THE BLOOD AND URINE OF PATIENTS WITH RECURRENT PYELONEPHRITIS
Authors: Korol L. , Stepanova N. , Migal L.
Published: June 2018

The aim of our study was to investigate the content of specific inflammatory proteins (C-reactive protein, fibronectin, cystatin C) in the blood and urine ofpatients with pyelonephritis, depending on the frequency of recurrences.

Patients and methods. A cross-sectional study was carried out and included 64 female patients with uncomplicated pyelonephritis, over 18 years (mean 32.3 ± 9 years). We determined C-reactive protein (CRP), fibronectin (FN), and cystatin C CysC) in the blood and urine of patients using test kits «Dialab» (Austria) by immunoturbidimetric method. After the examination patients were divided into II groups: I (n = 34) – women with recurrent pyelonephritis and II (n = 30) – patients with sporadic pyelonephritis (up to 2 times per year).

Results. The patients of group I showed the highest level of blood concentrations of CysC (p = 0.02) and a decrease of FN (p = 0.01). We found an inverse correlation between the number of recurrences of pyelonephritis per year and the blood level of FN (r = -0.42, p = 0.007). In addition, we determined a strong direct correlation between the urinary FN and blood level of CysC (r = 0.6, p <0.0001).

Conclusions. Decrease of FN and growth of CysC in the blood ofpatients with recurrent pyelonephritis is the result offrequent inflammatory activity. Determination of specific inflammatory proteins in the blood and urine ofpatients with pyelonephritis can be used as alternative markers for diagnosis and monitoring of disease
INFLUENCE OF ANGIOTENSIN-CONVERTING ENZYME RAMIPRIL ON INDICATORS OF TUBULO- INTERSTITIAL RENAL DAMAGE IN PATIENTS WITH CKDI-II: GLOMERULONEPHRITIS
Authors: Dolinnayä M.A. , Shekhovtseva T.G.
Published: June 2018

The aim: to examine the relations between morphological tubulointerstitial (TIT) parameters of kidney damage and neutrophil gelatinase-associated lipocalin (NGAL) and interleukin-18 (IL-18) of blood serum in patients with chronic glomerulonephritis (CGN) with saved renal function, and to estimate the therapeutic correction of identified changes using ACE inhibitor ramipril..

Materials and methods. The study included 81 patients with CGN. Patients were divided into 2 clinical groups: CGN patients with arterial hypertension (AH), CGN without AH. The average daily dose of ramipril in patients with AH was 12,8 ± 5,6 mg, in patients without hypertension – 2,5 mg.

We used kidney biopsy data for the analysis of renal damage on the following parameters: dystrophic and necrotic changes in tubular epithelium, thickening and/or cleavage of tubular basement membrane, presence ofcellular infiltration, interstitial fibrosis (IF). The level of NGAL and IL-18 in serum were determined by ELISA.

Results. We statistically confirmed a direct link between blood NGAL and IF (r=+0,65;p<0,05), blood IL-18 and dystrophic changes in tubular epithelium (r=+0,81;p<0,05). It was established that the kidney IF diagnostic using NGAL determination in serum is highly sensitive and specific, with an efficiency of 95,3 %, and dystrophic changes in tubular epithelium via serum IL-18 – 96,6 %.

Conclusion: serum levels of NGAL and IL-18 are sensitive markers of kidney TIT damage in patients with CGN with diagnostic efficiency up to 97 %. Under the influence of 24 weeks treatment with an ACE inhibitor ramipril we observed a significant decrease in levels of markers of kidney TIT damage, that confirmed nephroprotective effect of the drug.
ASSOCIATION OF HLA AND PROINFLAMMATORY CYTOKINES OF BLOOD IN PATIENTS WITH GLOMERULONEPHRITIS
Authors: Kolesnyk M. , Driyanska V. , Velychko M. , Drannik G. , Petrina O.
Published: June 2018

Introduction. Cytokines and HLA are of important part of immunogenesis of many diseases, therefore the analysis of these indices and this associations in dependence of glomerulonephritis (GN) can define their value as the additional prognostic markers.

Aim of the work is to determine the peculiarities of associations the high serum levels ofproinflammatory cytokines (TNF a, MCP-1, IL-18) and some HLA in phenotype to substantiate of chronic glomerulonephritis with nephrotic syndrome (CGN, NS) immunogenesis and to ascertain the additional prognostic markers.

Materials and methods. There was studied the HLA-antigens distribution in the 264 CGN, NS adult patients and 350 healthy donors by typing the lymphocytes with the aid of standard microlymphocytotoxic test (Terasaki’s test). Using IFA, the level of the proinflammatory cytokines was studied in the blood serum - MCP-1 in 39, IL-18 – 40 and TNF-a - 96patients.

Results. HLA-A23, -24, -B8, -38, -41, -44, DR1, -4, -w52 in adults patients have associations (RR>2) CGN, NS; the attributive risk (a>0,1) to develop GN detected in patients have A24, B8, DR 1, 4, w52. The relative risk (RR) to develop chronic renal failure (CRF) is in detection of HLA-10, -29, -30, -41, -51, DR4; attributive risk (AR) - A10.

The CGN, NS patients showed statistically higher level of the serum proinflammatory cytokines – TNF-6, IL-17, MCP-1 with more high indices of the TNF-a in patients with HLA-A23, -A28, -B44 (RR of CGN, NS), -A10 (AR of CRF), IL-18 - A24 (AR of CGN, NS) ma A10 (AR of CRF). The highest levels of MCP-1 detected in adults case have risk antigens - relative B41, attributive - A28, B8, and predictor of CRF B41, wich may be negative marker for prognosis.

Conclusion. It was to determine associations the serum levels of some cytokines and HLA in patients with CGN, NS. We think it appropriate to study HLA and proinflammatory cytokines TNF-a, IL-18 and MCP-1 in blood as additional negative prognostic predictors for the differentiating approach to treatment.
DIABETES MELLITUS, DIABETIC NEPHROPATHY, CHILDREN: CERTAINISSUES
Authors: Fomina A. P.
Published: June 2018

The report presented the current approach to diabetes and chronic kidney disease relationship, the problem of nutritional carbohydrates and their role in the diabetes epidemiological situation. The relevant diagnostic and genetic tests in diabetic nephropathy, particularly in children, were outlined. Evidence based treatment was considered according to renal function and pediatric specification.
PECULIARITIES OF IMMUNITY IN PATIENTS WITH DIABETES MELLITUS AND CHRONIC PYELONEPHRITIS
Authors: Busygina Yu. , Driyanska V. , Stepanova N. , Gaysenyuk F. , Lebed L. , Lavrenchuk O. , Kolesnyк M.
Published: June 2018

The pro- and antiinflammatory cytokines participate in antiinfective immunity, that is why it is necessary study their peculiarities in determination of the role in the immunopathogenesis of pyelonephritis, including complications of diabetes mellitus (DM).

The purpose of the work is to study the immune status and levels of pro- and antiinflammatory blood cytokines in the patients with chronic pyelonephritis (CPN), to determine the peculiarities in cases of DM.

Materials and methods. The immuno-enzymic method ELISA and the corresponding test-systems were used to study the levels of cytokines in blood and also standard immunity indices.

Results. The analysis showed the significant increase in levels of all studied pro- (TNF-б, MCP-1, IL-17, -23) and antiinflammatory (IL-4, TGF-в) cytokines in all patients with CPN and in every group – with (2) and without DM (3). In 3 gr. (CPN+DM) the CD4+/CD8+ are significantly lower, while in IgM, МСР-1 and TNF-б levels – higher than in the 1 gr. (DM without CPN), and IL-4 , NGAL – than in 2 gr. (CPN without DM).

Conclusion. The high level of cytokines (TNF-б, MCP-1 and IL-23) in blood confirms their important role in CPN+DM and CPN without DM. In patients with CPN+DM marked reduction in regulatory index (CD4+/CD8+) and higher levels of proinflammatory cytokines (TNF-б, MCP-1). Our investigation showed that NGAL level in serum was increased more significantly in 3 gr.; this fact may confirm that renal disfunction was more severe in patients with CPN+DM.
SYMPATHETIC HYPERACTIVITY IN CHRONIC KIDNEY DISEASE PATIENTS: FROM PATHOGENESIS TO TREATMENT
Authors: Stepanova N.
Published: June 2018

Sympathetic nervous system plays a crucial role in the development of cardiovascular complications in chronic kidney disease (CKD) patients. The aim of this review is to summarize up-to-date knowledge of the sympathetic hyperactivity in the pathogenesis of CKD, its clinical relevance, and as the options of current treatment.
HERPES VIRUS INFECTION AND GLOMERULONEPHRITIS IN CHILDREN
Authors: Kruglikov V. , Bagdasarova I. , Kruglikova I. , Vasilyeva I. , Zyubko O.
Published: June 2018

The article presents the analysis of the native and foreign literature data and also the results of our own research devoted to studying the role of herpes virus infection in the appearance and course of glomerulonephritis in children. There has been described pathogenesis and clinical aspects of virus associated glomerulonephritis in children. Much attention has been paid to the diagnostic methods of herpes virus infection in glomerulonephritis. The importance of association of immunosuppressive and antiviral therapy has been underlined.
ANTIBIOTIC- ASSOCIATED RISK THE REDUCTION OF LACTOBACILLUS SPP. IN THE INTESTINAL MICROBIOTA IN PATIENTS WITH URINARY TRACT INFECTIONS
Authors: Stepanova N. , Stashevska N. , Lebid L. , Savchenko S. , Kolesnyk M.
Published: June 2018

The aim of our study was to evaluate a risk reduction of indigenous gut microbiota in patients with urinary tract infection (UTI, associated with the intake of antibacterial medicinal products

Materials and methods. To this retrospective cohort case-control study were involved 233 women with UTI. The mean age in the patient population was 36.7 ± 15.6 years. Cases were defined as reducing the number of Lactobacillus spp. < 10 million colony forming units in 1 g offeces (mln. CFU/g). We evaluated the daily urinary oxalate excretion and bacteriological seeding feces. Investigational antibiotics were medicines which are generally used to treat the outpatient with UTI.

Results. The data analysis demonstrated an extremely high risk the reducing of Lactobacillus spp. colonization in the patient’s intestine. The use of ciprofloxacin was significantly associated with an availability of hyperoxaluria ( = 0.31,

p = 0.008).

Conclusions. Thus, the use of antibacterial medicinal products, even in prophylactic doses, by the patients with UTI leads to the destruction of the normal composition of gut microbiota, and, primarily, due to the fact that the content of Lactobacillus spp. is deceased.
QUALITY OF LIFE OF HEMODIALYSIS PATIENTS WITH ARTERIOVENOUS FISTULA
Authors: Rusyno I. , Tsiupiaka O.
Published: June 2018

Objective: to study the basic quality of life in patients with chronic kidney disease and their relationship, with different numbers formed arteriovenous fistula.

Materials and methods. Interviewed 56patients, who are treated with chronic hemodialysis department. To study the quality of life used (KDQOL-SF ™). Also used a questionnaire that enables presence fistula determine the impact on quality of life of patients.

Results. Using statistical method cluster analysis, made differentiation studied three groups - clusters, which differ in different levels of the indicators of QOL, namely «Patients middle-manifestation of physical and psychological indicators of QOL» – 40%, «Patients with low the manifestation of physical and psychological QOL parameters «- 25% of subjects, and» Patients with high level of physical and psychological manifestations of QOL performance «- 35% sample of of the patients.

Based on statistical comparative analysis revealed differences between the estimated quality of life in men and women. Role functioning due to physical condition in women is slightly lowered than men. In women, there is a greater level of pain than men. Men overall higher rate their health than women. Men are characterized by a higher level of social functioning than women.

Conclusions. Patients with chronic hemodialysis age have difficulties in physical functioning and performance of daily affairs. Mental health by increasing the biological age becomes stronger and fitter.

His attitude to the presence of fistula in upper limb showing men and women equally, that they feel discomfort and limitations in performing daily routine. The more surgeries performed to form vascular access in patients, the occurrence characteristically uncomfortable sensations in all aspects of life
PHARMACOKINETICS AND PHARMACODYNAMICS OF ANTIMICROBIAL AGENTS IN PATIENTS WITH IMPAIRED RENAL FUNCTION: AMINOGLYCOSIDES
Authors: Kolesnyk M. , Zakon K. , Dudarenko V.
Published: June 2018

This is review of aminoglycoside antibiotics pharmacokinetics and pharmacodynamics in patients with normal and impaired renal function. Dosing of different aminoglycosides in patients with different stage of renal function impairment and different modalities of renal replacement therapy are highlighted.
URINARY SYSTEM PATHOLOGY IN CHILDREN POPULATION OF KHARKIV REGION: MORBIDITY AND SOME RISK FACTORS
Authors: Muratov G. R. , Kolibaeva T. F. , Sorokina I. V. , Myroshnychenko M. S.
Published: June 2018

Urinary system pathology in children now is one of the most important problems of medical science.

The aim of this study – to analyze the morbidity and reveal the risk factors for development of urinary system diseases in children of Kharkiv region.

Materials and methods. The material of the study was the information (gender, age, weight and body length at birth, gestational age, clinical diagnosis), obtained during hospitalization of 663 patients aged from 3 months to 17 years in the nephrological center of Kharkiv regional children clinical hospital during the period from 2012 to 2013 years.

Results. The authors found that in children population of Kharkiv region among all diseases of urinary system a significant number of cases occur in congenital malformations, dismetabolic nephropathy, urolithiasis.

Conclusions. Regional risk factors for development of urinary system pathology in children are female and living in the village. Decreased weight and body length at birth are risk factors for development in children urinary system congenital malformations.
COLON OXALATE-DEGRADING BACTERIA COLONIZATION IN PATIENTS WITH RECURRENT PYELONEPHRITIS AND ITS RELATIONSHIP WITH ADMINISTRATION OF ANTIBIOTICS (PILOT STUDY)
Authors: Dzyubenko N. , Stashevska N. , Tolstanova G. , Sergiychuk T. , Akulenko I. , Stepanova N.
Published: June 2018

Antibiotic therapy and long-term antibiotic prophylaxis affect the normal flora of the gastrointestinal tract in the patients with recurrent pyelonephritis. It may disturb the balance in oxalate-degrading bacteria in colon and induce hyperoxaluria.

The aim of this study was to investigate the level of colon oxalate-degrading bacteria colonization inpatients with recurrentpyelonephritis and its association with administration ofantibiotics.

Patients and methods. The pilot observational cross-sectional study, involving 12 patients with uncomplicated recurrent pyelonephritis, non-stone formers. Oxalate-degrading bacteria in feces were identified by bacteriological culture method in the sodium oxalate reach medium.

Results. Oxalate-degrading bacteria were detected in feces of 6 (50%) surveyed patients. The number of oxalatedegrading bacteria ranged from 2,0 to 7,0 lg CFU/g in feces and depended on the time after the last administration of antibiotics (F = 4,6; P = 0.05). The levels of oxalate-degrading bacteria colonization and urinary oxalate excretion in surveyed women had a moderate inverse correlation (p =-0,6;P = 0.03).

Conclusions. The significantly higher urinary oxalate excretion in women non stone-formers with recurrent pyelonephritis may be associated with the application of antibiotics and a subsequent decolonization of oxalate-degrading bfcteria:     must befurther confirmed in ranaomizea study.
CHANGES OF MORPHOLOGY AND IMMUNOREACTIONS IN THE VESSELS OF KIDNEYS, HEART AND AORTA IN PATIENTS WITH CHRONIC KIDNEY DISEASE
Authors: Jakovtsova I.I. , Topchij I.I. , Daniluk S.V. , Kirienkow A.N. , Dunaievska М.М.
Published: June 2018

Chronic kidney diseases are etiology of complications in cardiovascular system. Structural and functional changes ofendothelium on a background ofimmunoreactions lies in basis ofthese disorders and complications.

The aim of research was to study of morphologicalfeatures of kidneys and immune reactions in the vessels of kidneys, heart, aorta inpatients with CDK.

Materials and methods. An autopsy material of the 20 dead’s age from 45 to 55 was investigated . The deceased had signs ofchronic desease of kidneys (CDK). Theprimary monoclonal antibody (PMA) company ofDAKO (Denmark) Rady - to - Use were used to reveal of features of cellular immunoreactions in the zones of inflammatoryprocess. We used microscope Primo Star (Carl Zeiss) with theprogram AxioCam (ERc 5s).

Results and discussion. Changes of kidneys vessels, coronary arteries and aorta were characterized of transformation similar with immune inflammation. Condition of endothelium was characterized disorder of endothelization

and manifest irregularity, lumpiness with outcrop of wide intercellular connections. All this proses bring to activation of apoptosis.

Conclusion. Disturbance of vessel morphology and activity of immunoinflammatory reaction can be one of main cause ofdeveloping cardio-vascular complication.
PERITONEAL DIALYSISINUKRAINE: 2009–2013
Authors: Saidakova N. , Kozlyuk N. , Nikolaenko S. , Stepanova N.
Published: June 2018

We analyzed the information of the state ofperitoneal dialysis in Ukraine for the period 2009–2013. The authors are grateful to all the main coordinators of the regional registries who had sent the relevant information to the SI «Institute of Nephrology NAMS of Ukraine», and contributed to the formation of the National Register.
ROLE OF HYPOXIA–INDUCED APOPTOSIS IN CHRONIC GLOMERULONEPHRITIS PROGRESSION IN CHILDREN
Authors: Maidannyk V.G. , Burlaka E.A. , Bagdasarova I.V. , Fomina S.P. , Nepomnyaschiy V.M.
Published: June 2018

Aim of the study: to study the indicators of cellular hypoxia and apoptosis in pediatric patients with nephritic type of chronic glomerulonephritis.

Material and methods: 52patients with active stage of nephrotic type ofChronic glomerulonephritis were inspected. All patients were divided into groups of Chronic Kidney Disease (CKD) by the level of glomerular filtration rate (GFR). Detection of the hypoxia–induced factor (HIF) and antiapoptotic factor Bcl–xL in serum performed using Western Blotting assay and immunohistochemically on material of kidney biopsies. Imaging was done using confocal laser microscopy.

Results: it has been found that the disease course is accompanied by increased levels of hypoxia–induced factor HIF–1a and decreased expression of antiapoptotic factor Bcl–xL (in plasma and on biopsies). Detected changes significantly depended on the degree of proteinuria and declining of glomerular filtration rate. Dependence between the levels of hypoxia–induced damages and level of kidney function impairment was documented. In children with Chronic Kidney Disease (SKDIst.) HIF–1a was at level 128.6±2.3% (P<0.01, compared to Control group), in children with CKD II–III st. – 141.3±1.9% (P<0.01, compared to Control group and CKD I st.). Level of antiapoptotic defense in children with nephrotic type of Chronic glomerulonephritis was related to the level of kidney function impairment as well. In group of patient with CKDIst. Bcl–xL expression was down–regulated to 75.1±2.2%, in group with CKDII–IIIst. — to 60.1+1.8% (P<0.01 and P<0.001, compared to Control group, respectively). The level of evaluated changes has a dependence on levels ofproteinuria and kidney function impairment.

Conclusion. Studied parameters might be used as predictors of unfavorable disease course.
ACTIVITY OF CHRONIC INFLAMMATION AND ENDOTHELIAL DAMAGE IN PATIENTS WITH CARDIAC VALVE CALCIFICATION IN DIALYSIS–DEPENDENT CHRONIC KIDNEY DISEASE
Authors: Susla O. B.
Published: June 2018

The purpose of the research was to identify the role of chronic inflammation in mechanisms of cardiac valve calcification (CVC) in patients undergoing chronic hemodialysis (HD) by determining the relation of inflammatory markers with valve calcification and the correlation of the latter with endothelial damage indices.

Methods. The research included 94patients undergoing chronic HD (males, 52, age, (46,4±11,2) years, duration of HD, (28,9±32,4) months). Patients with chronic glomerulonephritis (47,9 %) dominated. All subjects underwent echo– cardiographic examination for detection of CVC. The intensity of the inflammatory process was estimated by the serum content of fibrinogen (FG), amount of circulatory immune complexes (CICs), concentration of C–reactive protein (CRP) and middle molecules (MM). The nitric oxide (NO) production was studied on the plasma content of nitrite–anions (NO2–) by spectrophotometric method, the amount of circulating endothelial cells (CECs) in platelet rich plasma under the method (Hladovec J. et al., 1978), modified by (Susla A.B., Mysula I.R., 2011).

Results. The CRP, FG, CICs indices in patients with CVC exceeded in those without the calcification by 44,2 (р=0,009), 18,4 (р<0,001) and 17,2 % (р=0,002) accordingly. The dynamic of the MM with wave length of 254 nm (MM/254) and 280 nm (MM/280) had identical direction. For the first time a group of patients with CVC (n=42) had been identified with correlations between CECs and CRP indices (Rs=0,42, р=0,006), FG (Rs=0,31, р=0,043), CICs (Rs=0,55, р<0,001), MM/254 (Rs=0,36, р=0,018), MM/280 (Rs=0,42, р=0,005) as well as between the values of NO2– and CRP (Rs=–0,55, р<0,001), FG (Rs=–0,41, р=0,007), CICs (Rs=–0,41, р=0,008), MM/254 (Rs=–0,38, р=0,014), MM/280 (Rs=–0,34, р=0,029).

Conclusions. The valve calcification in HD patients combines with the activation of chronic inflammation, which manifests itself in accumulation of CRP, FG, CICs and MM, and the inflammation markers tightly correlate with endothelial damage and lack of NO
EFFICACY AND SAFETY OF IRON (III)–HYDROXIDE SUCROSE COMPLEX IN CORRECTION OF ANEMIA STAGE 5D CHRONIC KIDNEYDISEASE HEMODIALYSIS PATIENTS NOT TREATED BY ERYTHROPOIESIS–STIMULATING AGENTS (PROSPECTIVEANALYSIS)
Authors: Dudar І. О. , Gonchar I. I. , Shifris І. М. , Krasjuk Е. К. , Savchuk V. М. , Loboda О. М. , Pruskiy F. О. , Krot V. F. , Nechiporuk Т. М. , Alekseeva N. G.
Published: June 2018

The aim of study was to evaluat the efficacy and safety ofSUFER® (iron (III) sucrose complex) in correction of anemia stage 5D chronic kidney disease hemodialysis patients.

Methods. This study included thirty patients undergoing regular hemodialysis (mean age 48,81±3,24 years, mean duration of dialysis 30,43±9,25 months) with renal anemia and iron deficiency. All patients were treated with SUFER® intravenously 200 mg three times a week. Correction dose was determined according to the manufacturer’s recommendations.

Results. Mean level offerritin was significantly increased from 125,15 ± 21,46 ng / ml to 375,56 ± 64,12 ng / ml (p <0,001), transferrin saturation – from 17,48 ± 2,71% to 38,21 ± 4,90 ng / ml (p <0,001). 23 (76.67%) patients achieved target levels offerritin and transferrin, 12 (40% ) adverse events.

Conclusions. SUFER® is effective and safety drug in dialysis patients.
SERUM NEUTROPHIL GELATINASE - ASSOCIATED LIPOCALIN (NGAL) AS A MARKER OF TUBULOINTERSTITIAL DAMAGE IN PATIENTS WITH CHRONIC GLOMERULONEPHRITIS
Authors: Dolinnaya M.A.
Published: June 2018

Determining of serum creatinine level, glomerular filtration rate and albuminuria gives us no information about tubulointerstitial kidney damage. So it is especially important to search for non - invasive diagnostic methods that allow to diagnose early stages of tubulointerstitial kidney damage and start adequate treatment. This circumstance attempts to use neutrophil gelatinase - associated lipocalin (NGAL) to assess tubulointerstitial kidney damage in patients with chronic glomerulonephritis (CGN).

The aim: to analyze the morphological picture of the kidneys in patients with CGN with arterial hypertension (AH) and without, to investigate the relationship between serum NGAL and histological indexes of tubulointerstitial kidney damage in CGN patients with saved renal function.

Materials and methods. We examined 81 patients with CGN. Patients were divided into two clinical groups: CGN patients with AH, CGN without AH. We analyzed indicators of tubulointerstitial kidney damage according to vivo morphological study, level of serum NGAL.

Conclusion: there is a correlation of varying strength between serum NGAL and indexes of tubulointerstitial kidney damage in CGN patients, strong direct relationship is found between serum NGAL and IF. Diagnostics of IF by determing ofserum NGAL is a highly sensitive and specific method with the efficiency of95.3%. Increase ofserum NGAL above 5ng/ ml indicates a high probability of the IF in CGN patients.Results. Patients with AH have more pronounced histological damage of tubulointerstitial tissue than CGNpatients without AH. Serum NGAL levels are higher in CGN patients with AH and without than in the control group. We find a correlation between serum NGAL and indicators of tubulointer - stitial kidney damage. Analysis of the diagnostic operating characteristics of interstitial fibrosis (IF) by determining of serum NGAL shows that this method is highly sensitive and specific, with an efficiency of95,3%. Constructing ofa non - linear model revealed that increase of serum NGAL level above 5 ng/ml indicates a high probability of the IF in CGN patients.
PARENTERAL NUTRITION IN PATIENTS WITH ACUTE KIDNEY INJURY
Authors: Zakon K. , Berezyak T. , Radchenko G. , Romanova V.
Published: June 2018

This review is dedicated to changes in metabolism of major nutrients (proteins, fats and carbohydrates) in patients with acute kidney injury. Differences with other acute diseases and influence of renal replacement therapy are highlighted. Results of experimental and clinical trials are debated. The aim of nutritional support and macronutrients requirements are specified, as well as indications to parenteral nutrition, alternative approaches to control protein catabolism and influence of nutritional support on outcome.
SYSTEMIC EFFECTS OF LOCAL PRODUCTIONOFPRO- AND ANTI-FIBROGENIC MEDIATORS IN PERITONEAL DIALYSIS PATIENTS
Authors: Stepanova N. , Burdeyna O. , Driyanska V. , Savchenko V. , Kolesnyk M.
Published: June 2018

Peritonealfibrosis (PF) is a common morphological change in peritoneal dialysis (PD) patients. With the progression of PF, peritoneal membrane function is impaired, which leads to ultrafiltration failure. Results of studies in recent years show the crucial role of high production monocytic chemoattractant protein -1 (MCP-1) of peritoneal fibrosis in PD-patients. The aim was to determine intraperitoneally production ofIL-10 and MCP-1 and its relationship with clinical and laboratory parameters of adequacy of dialysis.

Patients and methods. A prospective observational study involved 18patients with CKD stage Vwith non-diabetic nephropathy, were treated with continuous ambulatory peritoneal dialysis (PAPD). The limits of the normal range (reference range) were obtained from the survey results of 10 conditionally healthy individuals. ELISA method in serum and peritoneal dialysis effluent (PDE) investigated levels ofIL-10 and MSP-1. PAPD adequacy indicators evaluated by determining the concentration of urea and creatinine in plasma, urine and dialysate, calculated weekly creatinine clearance (CrCl), dialysis (Ш/ Vd), renal (Ш / Vr) and шА weekly urea clearance (Kt/ V). nmstep@ukr.net 

Results. In all of the PD-patients compared to healthy donors conventionally, there was a significant increase in concentration of the cytokines. We determined the positive regression relationship between the concentration of MCP-1 and IL-10 in PDE and the level of total weekly (Kt / V) and renal (Kt / Vr).

Conclusions. We determined the regression dependence, which allowed to identify clinical parameters that are most closely (statistically significant) related to intraperitoneal production of MCP-1 and IL-10.
ANADOLU ARISI (Apis mellifera anatoliaca)’NIN BAZI ÖNEMLİ ÖZELLİKLERİ
Authors: Sıralı Recep
Published: June 2018

Anadolu arısı (Apis mellifera anatoliaca) Türkiye’deki
en yaygın arı ırkıdır. Bu arı ırkı, kuzeydoğu ve güneydoğu bölgeleri hariç
ülkenin orta kısımlarındaki yerli arı popülâsyonunu oluşturmaktadır. Bu arı
ırkı  doğu arılarının bir dalı olarak
sınıflandırılmıştır. Son zamanlarda yapılan genetik çalışmalar, bu bal arısı
ırkının Doğu Avrupa arılarının bir dalı olduğunu doğrulamıştır. Renk, verim,
morfolojik, fizyolojik ve davranış özellikleri bakımından büyük varyasyon
gösteren Anadolu arısı, çeşitli bölgelere adapte olmuş birçok ekotipe sahiptir.
Anadolu arı ırkının her ekotipi endemik çevresel koşullara sahip yaşam alanında
farklı özellikler ortaya koymaktadır. Anadolu arı ırkının en yaygın ekotipleri
Muğla arısı ve Orta Anadolu arısıdır. Anadolu arıları kirli sarı renkte ve
küçük vücut yapısına sahiptir. İtalyan, Karniyol ve Kafkas arılarına oranla
oldukça hırçın bir ırktır. Bu arı ırkı bazı çok iyi özelliklere de sahiptir.
Nektar toplama, kışlama yeteneği ve oğul eğilimi yüksek fakat yağmacılık
eğilimi düşüktür. Ayrıca kış döneminde az bal tüketen Anadolu bal arısı ırkı,
uygun koşullarda hızlı gelişir. Besin durumuna bağlı olarak iyi larva besleme
eğilimi Anadolu arısının önemli davranış özelliklerindendir. Bunlara ek olarak
kovan içerisinde ilave petek örme ve propolisi fazla taşıyıp kullanma gibi arzu
edilmeyen özelliklere sahiptir.
ARI ÜRÜNLERİ VE HAYVAN BESLEMEDE KULLANIMI
Authors: SUR ARSLAN Aslıhan , BİRBEN Nurgül , TATLI SEVEN Pınar , SEVEN İsmail
Published: June 2018

Arıcılık

aktivitelerinden bal, polen, propolis, arı sütü ve arı zehiri gibi ürünler elde

edilmektedir. Bu ürünler, halk arasında birçok hastalığın tedavisinde

kullanılmaktadır. Apiterapi adı verilen arı ürünleri ile tedavi yöntemleri son

zamanlarda gelişme göstermektedir. Antibakteriyel, antiviral, antikanserojen,

antioksidan, antiromatizmal v.b. birçok etkisi araştırılmış bu ürünler hayvan besleme

konusunda da son zamanlarda ilgi görmektedir. Bilindiği gibi antibiyotiklerin

yem katkı maddesi olarak kullanımının yasaklanması sebebi ile antibiyotiğe

alternatif doğal ürünlerin kullanımı yaygınlaşmaktadır. Propolis, polen, arı

sütü ve arı zehiri ile yapılan çalışmalar göstermektedir ki bu ürünler

hayvanların yem tüketimini, canlı ağırlığını ve yemden yararlanma oranını

artırarak performans üzerinde olumlu etkiye sebep olabilmektedir. Arı ürünleri

gelişmeyi artırıcı bu özellikleri ile alternatif ürün olarak kullanılabilecek

doğal ürünlerdir. Bu derlemede başta propolis olmak üzere polen, arı sütü ve

arı zehiri gibi doğal ürünlerin hayvan beslemede etkisinin araştırıldığı

çalışmaların derlenerek kullanımları hakkında bilgi verilmesi amaçlanmıştır. 