bluuebunny/crossref_metadata_2025 huggingface dataset:
Lapatinib Suppresses HER2-Overexpressed Cholangiocarcinoma and Overcomes ABCB1– Mediated Gemcitabine Chemoresistance
Authors: Bai Zhiqing , Guo Zhiying , Liu Jiaxing , Chen Yu-Ann , Lu Qian , Zhang Ping , Hong Lili , Wang Yunfang , Dong Jiahong
Published: April 2022

Background

Recent breakthroughs in cholangiocarcinoma (CCA) genomics have led to the discovery of many unique identifying mutations, of which HER2 has been found to be overexpressed specifically in cases of extrahepatic CCA. However, whether or not lapatinib (an oral tyrosine kinase inhibitor selective for inhibition of HER2), or a combination of lapatinib and gemcitabine, exerts inhibitory effects on HER2-overexpressed CCA is still unclear.

Methods

The effect of lapatinib and a lapatinib-gemcitabine combination treatment on CCA was determined using organoid and cell line models. Cell cycle arrest, apoptosis and proteins involving HER2-dependent downstream signaling pathways were analyzed to assess the effect of lapatinib on HER2+ CCA. The synergistic effect of lapatinib and gemcitabine was interpreted by docking analysis, ABCB1-associated ATPase assay, rhodamine transport assay and LC-MS/MS analyses.

Results

dFdCTP, the active metabolite of gemcitabine, is proved to be the substrate of ABCB1 by docking analysis and ATPase assay. The upregulation of ABCB1 after gemcitabine treatment accounts for the resistance of gemcitabine. Lapatinib exerts a dual effect on HER2-overexpressed CCA, suppressing the growth of CCA cells by inhibiting HER2 and HER2-dependent downstream signaling pathways while inhibiting ABCB1 transporter function, allowing for the accumulation of active gemcitabine metabolites within cells.

Conclusions

Our data demonstrates that lapatinib can not only inhibit growth of CCA overexpressing HER2, but can also circumvent ABCB1-mediated chemoresistance after gemcitabine treatment. As such, this provides a preclinical rationale basis for further clinical investigation into the effectiveness of a combination treatment of lapatinib with gemcitabine in HER2-overexpressed CCA.
Toripalimab in Combination With Induction Chemotherapy and Subsequent Chemoradiation as First-Line Treatment in Patients With Advanced/Metastatic Esophageal Carcinoma: Protocol for a Single-Arm, Prospective, Open-Label, Phase II Clinical Trial (TR-EAT)
Authors: Wu Lei , Wang Yi , Li Baisen , Wan Gang , Liang Long , Li Tao , Lang Jinyi , Wang Qifeng
Published: April 2022

Immune checkpoint inhibitor therapy combined with chemotherapy is safe and effective in treating advanced esophageal carcinoma; however, some patients still experience tumor progression and/or metastasis. Whether the addition of radiotherapy to immunotherapy combined with chemotherapy improves the prognosis of patients with advanced/metastatic esophageal carcinoma needs to be investigated. In the present study, we developed a protocol for our clinical trial indicating that toripalimab combined with induction chemotherapy followed by chemoradiotherapy can safely prolong survival in patients with stage IV esophageal carcinoma. This open-label, single-arm, phase II trial will include patients with unresectable stage IV esophageal squamous cell carcinoma who have not received prior systemic therapy. The patients will be treated with two cycles of toripalimab (240 mg, 1 day before chemotherapy, Q3W) combined with induction chemotherapy (paclitaxel, 135–175 mg/m2 + carboplatin, area under the curve = 4–6, day 1, intravenous, Q3W). Thereafter, they will undergo two cycles of the aforementioned treatment with concurrent radiotherapy (30–50 Gy in 15–25 fractions), followed by toripalimab (240 mg, day 1, Q3W) for 1 year. The primary outcome measure will be progression-free survival; the secondary outcome measures will include the objective response rate, disease control rate, duration of remission, 1- and 2-year overall survival rates, safety and tolerability, and changes in health-related quality of life. The study protocol was approved by the Ethics Committee of Sichuan Cancer Hospital (SCCHEC-02-2021-021). The trial is underway in accordance with the Declaration of Helsinki.

Clinical Trial Registration

http://www.chictr.org.cn/showproj.aspx?proj=126830, identifier ChiCTR2100046715.
Case Report: Advanced Skeletal Muscle Imaging in S-Adenosylhomocysteine Hydrolase Deficiency and Further Insight Into Muscle Pathology
Authors: Petković Ramadža Danijela , Kuhtić Ivana , Žarković Kamelija , Lochmüller Hanns , Čavka Mislav , Kovač Ida , Barić Ivo , Prutki Maja
Published: April 2022

Introduction

S-Adenosylhomocysteine hydrolase deficiency (SAHHD) is a rare inherited multisystemic disease with muscle involvement as one of the most prominent and poorly understood features. To get better insight into muscle involvement, skeletal muscles were analyzed by magnetic resonance imaging (MRI) and MR spectroscopy (MRS) in three brothers with SAHHD in the different age group.

Method

The study was based on analysis of MRI and MRS of skeletal muscles of the lower and the proximal muscle groups of the upper extremities in three SAHHD patients.

Results

Three siblings presented in early infancy with similar signs and symptoms, including motor developmental delay. All manifested myopathy, more pronounced in the lower extremities and the proximal skeletal muscle groups, and permanently elevated creatine kinase. At the time of MRI and MRS study, the brothers were at the age of 13, 11, and 8 years, respectively. MRI revealed lipid infiltration, and the MRS curve showed an elevated muscle lipid fraction (higher peak of lipid), which increased with age, and was more prominent in the proximal skeletal muscles of the lower extremities. These results were consistent with muscle biopsy findings in two of them, while the third patient had no specific pathological changes in the examined muscle tissue.

Conclusions

These findings demonstrate that an accessible and non-invasive method of MRI and MRS is useful for an insight into the extent of muscle involvement, monitoring disease progression, and response to treatment in SAHHD.
Is There a Minimum Effective Dose for Vascular Occlusion During Blood Flow Restriction Training?
Authors: Das Arpan , Paton Bruce
Published: April 2022

Background

Blood flow restriction (BFR) training at lower exercise intensities has a range of applications, allowing subjects to achieve strength and hypertrophy gains matching those training at high intensity. However, there is no clear consensus on the percentage of limb occlusion pressure [%LOP, expressed as a % of the pressure required to occlude systolic blood pressure (SBP)] and percentage of one repetition max weight (%1RM) required to achieve these results. This review aims to explore what the optimal and minimal combination of LOP and 1RM is for significant results using BFR.

Method

A literature search using PubMed, Scopus, Wiley Online, Springer Link, and relevant citations from review papers was performed, and articles assessed for suitability. Original studies using BFR with a resistance training exercise intervention, who chose a set %LOP and %1RM and compared to a non-BFR control were included in this review.

Result

Twenty-one studies met the inclusion criteria. %LOP ranged from 40 to 150%. %1RM used ranged from 15 to 80%. Training at 1RM ≤20%, or ≥ 80% did not produce significant strength results compared to controls. Applying %LOP of ≤50% and ≥ 80% did not produce significant strength improvement compared to controls. This may be due to a mechanism mediated by lactate accumulation, which is facilitated by increased training volume and a moderate exercise intensity.

Conclusion

Training at a minimum of 30 %1RM with BFR is required for strength gains matching non-BFR high intensity training. Moderate intensity training (40–60%1RM) with BFR may produce results exceeding non-BFR high intensity however the literature is sparse. A %LOP of 50–80% is optimal for BFR training.
Long-Term Stability of Bacterial Associations in a Microcosm of Ostreococcus tauri (Chlorophyta, Mamiellophyceae)
Authors: Vacant Sophie , Benites L. Felipe , Salmeron Christophe , Intertaglia Laurent , Norest Manon , Cadoudal Adrien , Sanchez Frederic , Caceres Carlos , Piganeau Gwenael
Published: April 2022

Phytoplankton–bacteria interactions rule over carbon fixation in the sunlit ocean, yet only a handful of phytoplanktonic–bacteria interactions have been experimentally characterized. In this study, we investigated the effect of three bacterial strains isolated from a long-term microcosm experiment with one Ostreococcus strain (Chlorophyta, Mamiellophyceae). We provided evidence that two Roseovarius strains (Alphaproteobacteria) had a beneficial effect on the long-term survival of the microalgae whereas one Winogradskyella strain (Flavobacteriia) led to the collapse of the microalga culture. Co-cultivation of the beneficial and the antagonistic strains also led to the loss of the microalga cells. Metagenomic analysis of the microcosm is consistent with vitamin B12 synthesis by the Roseovarius strains and unveiled two additional species affiliated to Balneola (Balneolia) and Muricauda (Flavobacteriia), which represent less than 4% of the reads, whereas Roseovarius and Winogradskyella recruit 57 and 39% of the reads, respectively. These results suggest that the low-frequency bacterial species may antagonize the algicidal effect of Winogradskyella in the microbiome of Ostreococcus tauri and thus stabilize the microalga persistence in the microcosm. Altogether, these results open novel perspectives into long-term stability of phytoplankton cultures.
Handheld Multifunctional Fluorescence Imager for Non-invasive Plant Phenotyping
Authors: Zhang Ruochong , Koh Sally Shuxian , Teo Mark Ju Teng , Bi Renzhe , Zhang Shuyan , Dev Kapil , Urano Daisuke , Dinish U. S. , Olivo Malini
Published: April 2022

Fluorescence imaging has shown great potential in non-invasive plant monitoring and analysis. However, current systems have several limitations, such as bulky size, high cost, contact measurement, and lack of multifunctionality, which may hinder its applications in a wide range of settings including indoor vertical farming. Herein, we developed a compact handheld fluorescence imager enabling multipurpose plant phenotyping, such as continuous photosynthetic activity monitoring and non-destructive anthocyanin quantification. The compact imager comprises of pulse-amplitude-modulated multi-color light emitting diodes (LEDs), optimized light illumination and collection, dedicated driver circuit board, miniaturized charge-coupled device camera, and associated image analytics. Experiments conducted in drought stressed lettuce proved that the novel imager could quantitatively evaluate the plant stress by the non-invasive measurement of photosynthetic activity efficiency. Moreover, a non-invasive and fast quantification of anthocyanins in green and red Batavia lettuce leaves had excellent correlation (>84%) with conventional destructive biochemical analysis. Preliminary experimental results emphasize the high throughput monitoring capability and multifunctionality of our novel handheld fluorescence imager, indicating its tremendous potential in modern agriculture.
Natural Deterioration Processes of Salix psammophila Sand Barriers in Atmospheric Exposure Section
Authors: Wang Ruidong , Dang Xiaohong , Gao Yong , Yang Xia , Liang Yumei , Zhao Chen , Duan Xiaoting
Published: April 2022

The atmospheric conditions of desert environments are important for the protection of Salix psammophila Sand Barrier, and these conditions can affect and change the structure and performance of the sand barrier, causing them to lose their wind proofing and sand fixing benefits. In this study, we have first examined the key environmental factors that affect the exposure of S. psammophila sand barrier. Then, we assessed how key factors in the desert atmospheric environment affect structural aging and performance. The relative crystallinity and chemical composition changes in the sand barrier were measured by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS), and the main degradation factors and processes were discussed. The results showed that the degradation degree of the exposed S. psammophila sand barrier was mainly affected by moisture and ultraviolet radiation. Lignin was the main component and the source of photodegradation and photodiscoloration observed in the sand barrier. However, other polysaccharides, such as cellulose and hemicellulose, were less affected by photodegradation. The stress generated by alternating desorption-absorption was the main cause of the expansion and contraction, deformation, cracking, and warping observed in S. psammophila sand barrier. We also found a series of irreversible changes and losses that occurred, which affected the natural material properties of S. psammophila sand barrier exposed to atmospheric conditions for several years. Exposure times between 5 and 7 years were the most important turning point in time for determining the deterioration of the S. psammophila sand barrier. Our results highlighted the importance of the interactions between atmospheric factors and the exposed atmospheric sections of the S. psammophila sand barrier from the perspective of environmental effects. However, the exact mechanisms of the sand barrier deterioration still need further investigation. Nevertheless, our overall findings advanced the current understanding of the environmental effects of S. psammophila sand barrier for ecological restoration and desertification reversal, especially in stressful desert environments.
The Characteristics of Radial Growth and Ecological Response of Caragana korshinskii Kom. Under Different Precipitation Gradient in the Western Loess Plateau, China
Authors: Che Cunwei , Xiao Shengchun , Ding Aijun , Peng Xiaomei , Su Jingrong
Published: April 2022

Understanding the temporal-spatial variability of tree radial growth and ecological response is the basis for assessing forest vulnerability in sight of climate change. We studied stands of the shrub Caragana korshinskii Kom. at four sampling sites (natural forest CL and plantation forests XZJ, CK and TPX) that spanned the different precipitation gradient (180–415 mm) across China’s western Loess Plateau, and demonstrated its radial growth dynamics and ecological response. We found that the growth of natural C. korshinskii in arid regions have adapted and cope with regional environmental changes and radial growth was less affected by drought stress. However, the growth of planted C. korshinskii was significantly affected by drought stress in arid and semi-arid regions, especially during the growing season (from June to September). Variations in radial growth rates and growth indicators such as shrub height, canopy area are consistent with the climate-growth relationship. With increase of precipitation, the limiting of drought on the growth of planted C. korshinskii gradually decreased and the amount of radial growth variation explained by drought decreased from 53.8 to 34.2% and 22.3% from 270 to 399 and 415 mm of precipitation, respectively. The age-related radial growth trend shows that radial growth increased until 4 years of age, then decreased rapidly until 12–14 years of age, and then eventually tend to stabilized. In the context of climate warming and humidification, increased precipitation and regular branch coppicing management at around 12 years old will help to mitigate the limitation of drought on the growth of C. korshinskii. Moreover, the initial planting density should be tailored to local precipitation conditions (below 5,000 shrubs per hectare). The above results have important practical significance for the maintenance of the stability and sustainable management of plantation forests in the western Loess Plateau.
OsDDM1b Controls Grain Size by Influencing Cell Cycling and Regulating Homeostasis and Signaling of Brassinosteroid in Rice
Authors: Guo Mingliang , Zhang Wenchao , Mohammadi Mohammad Aqa , He Zhimei , She Zeyuan , Yan Maokai , Shi Chao , Lin Lingwei , Wang Aqiong , Liu Jindian , Tian Dagang , Zhao Heming , Qin Yuan
Published: April 2022

Snf2 family proteins are the crucial subunits of chromatin-remodeling complexes (CRCs), which contributes to the biological processes of transcription, replication, and DNA repair using ATP as energy. Some CRC subunits have been confirmed to be the critical regulators in various aspects of plant growth and development and in epigenetic mechanisms such as histone modification, DNA methylation, and histone variants. However, the functions of Snf2 family genes in rice were poorly investigated. In this study, the relative expression profile of 40 members of Snf2 family in rice was studied at certain developmental stages of seed. Our results revealed that OsCHR741/OsDDM1b (Decrease in DNA methylation 1) was accumulated highly in the early developmental stage of seeds. We further analyzed the OsDDM1b T-DNA insertion loss-of-function of mutant, which exhibited dwarfism, smaller organ size, and shorter and wider grain size than the wild type (Hwayoung, HY), yet no difference in 1,000-grain weight. Consistent with the grain size, the outer parenchyma cell layers of lemma in osddm1b developed more cells with decreased size. OsDDM1b encoded a nucleus, membrane-localized protein and was distributed predominately in young spikelets and seeds, asserting its role in grain size. Meanwhile, the osddm1b was less sensitive to brassinosteroids (BRs) while the endogenous BR levels increased. We detected changes in the expression levels of the BR signaling pathway and feedback-inhibited genes with and without exogenous BR application, and the alterations of expression were also observed in grain size-related genes in the osddm1b. Altogether, our results suggest that OsDDM1b plays a crucial role in grain size via influencing cell proliferation and regulating BR signaling and homeostasis.
Environmental and Genetic Factors Involved in Plant Protection-Associated Secondary Metabolite Biosynthesis Pathways
Authors: Zhan Xiaori , Chen Zhehao , Chen Rong , Shen Chenjia
Published: April 2022

Plant specialized metabolites (PSMs) play essential roles in the adaptation to harsh environments and function in plant defense responses. PSMs act as key components of defense-related signaling pathways and trigger the extensive expression of defense-related genes. In addition, PSMs serve as antioxidants, participating in the scavenging of rapidly rising reactive oxygen species, and as chelators, participating in the chelation of toxins under stress conditions. PSMs include nitrogen-containing chemical compounds, terpenoids/isoprenoids, and phenolics. Each category of secondary metabolites has a specific biosynthetic pathway, including precursors, intermediates, and end products. The basic biosynthetic pathways of representative PSMs are summarized, providing potential target enzymes of stress-mediated regulation and responses. Multiple metabolic pathways share the same origin, and the common enzymes are frequently to be the targets of metabolic regulation. Most biosynthetic pathways are controlled by different environmental and genetic factors. Here, we summarized the effects of environmental factors, including abiotic and biotic stresses, on PSM biosynthesis in various plants. We also discuss the positive and negative transcription factors involved in various PSM biosynthetic pathways. The potential target genes of the stress-related transcription factors were also summarized. We further found that the downstream targets of these Transcription factors (TFs) are frequently enriched in the synthesis pathway of precursors, suggesting an effective role of precursors in enhancing of terminal products. The present review provides valuable insights regarding screening targets and regulators involved in PSM-mediated plant protection in non-model plants.
Research on the Influence of Tolerance of Opportunistic Behaviors of Channel Boundaries on the Choice of Response Strategies
Authors: Chen Jinsong , Liu Zhaoxia , Hu Ruoqian
Published: April 2022

In the Chinese society, border agents in channel transactions will choose different opportunistic behavior response strategies to the tolerance of other members based on the relationship between the two parties. Based on 206 valid questionnaires collected, structural equation model and regression analysis were used to investigate the influence of opportunistic behavior tolerance on response strategy selection. The results show that the channel boundary personnel's tolerance to opportunistic behavior (based on work or personal) negatively influences their choice of a positive response strategy and positively influences their choice of a negative response strategy. Among the mediating effects of contract formulation, transaction terms have a positive effect on the choice of negative response strategies based on the work and individual opportunistic behavior tolerance and have no mediating effect on the choice of positive response strategies; the contingency clause has no mediating effect on the choice of positive response strategies based on individual opportunistic behavior tolerance.
The Effects of Psychological Interventions on Symptoms and Psychology of Functional Dyspepsia: A Systematic Review and Meta-Analysis
Authors: Wei Zhongcao , Xing Xin , Tantai Xinxing , Xiao Cailan , Yang Qian , Jiang Xiaosa , Hao Yujie , Liu Na , Wang Yan , Wang Jinhai
Published: April 2022

Background

The effects of psychological interventions on symptoms and psychology of functional dyspepsia (FD) remain unclear. We aimed to comprehensively evaluate the effects of psychological interventions on symptoms and psychology of FD.

Methods

We searched the PubMed, Cochrane Library, and Embase electronic databases for randomized controlled trials (RCTs) evaluating the role of psychological interventions in FD patients published before July 2021. Standardized mean differences (SMDs), risk ratios (RRs) and 95% confidence intervals (CIs) were calculated by a random effects model. Subgroup analyses and sensitivity analyses were also performed.

Results

Fourteen RCTs with a total of 1,434 FD patients were included. Compared with the control group, psychological interventions were significantly more likely to symptom improvement [RR = 1.74, 95% CI (1.12, 2.72), p = 0.01], relieve gastrointestinal symptoms scores at follow up [SMD = −1.06, 95% CI (−1.55, −0.57), p < 0.0001], relieve gastrointestinal symptoms scores at end of treatment [SMD = −0.98, 95% CI (−1.29, −0.67), p < 0.001], decrease anxiety [SMD = −0.8, 95% CI (−1.38, −0.22), p = 0.006] and depression levels [SMD = −1.11, 95% CI (−1.62, −0.61), p < 0.001]. The results of the subgroup analysis showed that psychotherapy was more likely to symptom improvement, relieve gastrointestinal symptoms scores and decreased depression levels compared to the control.

Conclusions

Psychological interventions may be effective in alleviating the symptoms and psychology of FD, but the effect appears to be limited to psychotherapy with fewer trials for other psychological interventions. More data from high-quality RCTs are needed to confirm their use in the treatment of FD.
Predictability of Seasonal Mood Fluctuations Based on Self-Report Questionnaires and EEG Biomarkers in a Non-clinical Sample
Authors: Höller Yvonne , Urbschat Maeva Marlene , Kristófersson Gísli Kort , Ólafsson Ragnar Pétur
Published: April 2022

Induced by decreasing light, people affected by seasonal mood fluctuations may suffer from low energy, have low interest in activities, experience changes in weight, insomnia, difficulties in concentration, depression, and suicidal thoughts. Few studies have been conducted in search for biological predictors of seasonal mood fluctuations in the brain, such as EEG oscillations. A sample of 64 participants was examined with questionnaires and electroencephalography in summer. In winter, a follow-up survey was recorded and participants were grouped into those with at least mild (N = 18) and at least moderate (N = 11) mood decline and those without self-reported depressive symptoms both in summer and in winter (N = 46). A support vector machine was trained to predict mood decline by either EEG biomarkers alone, questionnaire data from baseline alone, or a combination of the two. Leave-one-out-cross validation with lasso regularization was used with logistic regression to fit a model. The accuracy for classification for at least mild/moderate mood decline was 77/82% for questionnaire data, 72/82% for EEG alone, and 81/86% for EEG combined with questionnaire data. Self-report data was more conclusive than EEG biomarkers recorded in summer for prediction of worsening of depressive symptoms in winter but it is advantageous to combine EEG with psychological assessment to boost predictive performance.
Depressive Symptoms Among Children and Adolescents in China During the Coronavirus Disease-19 Epidemic: A Systematic Review and Meta-Analysis
Authors: Chen Jianghe , Yang Kun , Cao Yujia , Du Yun , Wang Ningqun , Qu Miao
Published: April 2022

Background

The Coronavirus Disease-19 (COVID-19) pandemic negatively impacts mental health. Some published studies have investigated the prevalence of depression among children and adolescents in China during the pandemic. However, the results vary widely. We aimed to systematically analyze and estimate the prevalence of depressive symptoms and attempted to reveal the reasons for prevalence variety in previous studies.

Methods

Published studies were searched in PubMed, Embase, Cochrane Central, the Chinese Scientific Journal Database (VIP Database), China National Knowledge database (CNKI), and the WanFang database from December 2019 to May 2021. The quality of all included studies was assessed by the Joanna Briggs Institute (JBI) checklist and the American Agency for Health Care Quality and Research’s (AHRQ) cross-sectional study quality evaluation items. Meta-analysis was performed using random-effects modeling.

Results

Of the 1,708 references screened, 13 related reports that involve 41,729 participants were included. The results suggested that the pooled prevalence of depressive symptoms among Chinese children and adolescents during the COVID-19 epidemic was 28.6%. Subgroup analyses showed that the pooled prevalence was highest among the studies using the Patient Health Questionnaire (PHQ)-9 (46.8%) and lowest among these using Depression Self-Rating Scale for Children (DSRSC) (11.4%). All studies using PHQ-9 set the cutoff at 5 points instead of 10. The pooled prevalence of studies that include primary school students was lower (16.5%) than that of studies excluding primary school students (39.1%).

Conclusion

The meta-analysis suggests that depressive symptoms were relatively prevalent among Chinese children and adolescents during COVID-19, especially among the secondary school students. The suitable screening tools and cutoff should be carefully chosen in the survey.
Parents and Children Should Be More Active Together to Address Physical Inactivity and Sedentary Behaviours
Authors: Rainham Daniel G , Bennett Mark , Blanchard Christopher M , Kirk Sara FL , Rehman Laurene , Stone Michelle , Stevens Daniel
Published: April 2022

Increasing rates of physical inactivity and sedentary behaviours among children and the youth are important determinants of chronic disease. Supporting children's participation in organised physical activities like sports has been promoted as a public health strategy to increase physical activity. Evidence shows that successful interventions are family-focused, although research on how parental eating and physical activity behaviours influence children's behaviours is deficient. In this commentary, we argue that interventions for countering physical inactivity and sedentary behaviours should include greater focus on home and social environments, specifically the influence and involvement of parents, siblings, and friends in supporting these health behaviours. We conclude that the design of interventions to prevent chronic diseases in children should also consider more carefully the conditions in which the behaviours of children and their parents occur. This means encouraging parents and children to be active together to address physical inactivity and sedentary behaviours, while being mindful of unintended consequences of focusing on one behaviour over another.
The Effect of Household Dependency Ratio on the Mental Health of Workforce: Evidence From China
Authors: Fang Dongli
Published: April 2022

Based on the Chinese General Social Survey (CGSS) 2010, this article investigates the relationship between household dependency ratio and the mental health of household workforce. The empirical results verify the negative impacts of both household old-age dependency ratio and child dependency ratio on the mental health of the workforce and find that the negative effect of old-age dependency ratio is greater than that of child dependency ratio. Moreover, the depression source of the young workforce mainly comes from the child dependency ratio, while the depression source of the older workforce comes from the old-age dependency ratio. The mental health of the workforce in poor regions is impaired by the household dependency burden, but we found no same evidence in rich regions. The old-age dependency ratio negatively affects the mental health of the workforce due to the household healthcare burden, while the child dependency ratio negatively affects the mental health of the workforce due to the education expenditure pressure. Our findings provide evidence on how household structure affects the welfare of the family, and give implications to the improvement of residents' health.
How Do Intergenerational Economic Support, Emotional Support and Multimorbidity Affect the Catastrophic Health Expenditures of Middle-Aged and Elderly Families?–Evidence From CHARLS2018
Authors: Tang Shaoliang , Yao Ling , Li Zhengjun , Yang Tongling , Liu Meixian , Gong Ying , Xu Yun , Ye Chaoyu
Published: April 2022

Objectives

The elderly face multiple vulnerabilities such as health, economy and society, and are prone to catastrophic health expenditures. This study aims to analyze the impact of children's intergenerational economic support, emotional support, and illness on the catastrophic health expenditures of middle-aged and elderly families.

Methods

Using China Health and Retirement Longitudinal Study (CHARLS 2018) data to calculate the catastrophic health expenditure of Chinese households as the dependent variable. Taking children's intergenerational economic support, emotional support and multimorbidity as core independent variables, gender, age, marital status, medical insurance and other variables as control variables, and perform logistic regression analysis. According to the heterogeneity analysis of age and gender, the impact of intergenerational economic support, emotional support and multimorbidity on the catastrophic health expenditure of middle-aged and elderly families is explored.

Results

When catastrophic health expenditures occur in middle-aged and elderly families, the children's intergenerational economic support will increase significantly, especially in families with members aged 60–74. Children's emotional support can effectively reduce the risk of catastrophic health expenditures for middle-aged and elderly families. Compared with children's intergenerational economic support and emotional support, the impact of multimorbidity on the catastrophic health expenditures of middle-aged and elderly families is the most significant. Suffering from multimorbidity can increase the risk of catastrophic health expenditures for middle-aged and elderly families, especially families with male members suffering from multiple diseases.

Conclusions

It is recommended that we should do a good job in popularizing the knowledge of chronic diseases to minimize the occurrence of multimorbidity. The government should establish group medical insurance related to chronic disease diagnosis. According to the severity of the disease or the special circumstances of the patient, the level of medical insurance reimbursement is divided in detail, especially for chronic disease clinics and drug reimbursement. Children should be encouraged to strengthen the emotional connection and effective care of the elderly, focusing on the elderly 60–74 years old, in order to reduce their care pressure and maintain the physical and mental health of the elderly.
Amylolytic Fungi in the Ethnic Beer Starter “emao” and Their Beer-Producing Attributes
Authors: Boro Nitesh , Narzary Diganta
Published: April 2022

Naturally occurring autochthonous microbes associated with ethnic beer starters are diverse and important as they play different functional roles in beer fermentations. The study on culturable microbes from the ethnic rice beer starter “emao” of the Bodo community of Assam is limited. Here we isolated and identified the culturable fungal diversity associated with emao and screened them for beer-producing capability from glucose and starch substrates. Based on morphology and molecular characterization, the species identified were Candida glabrata (Cgla_RF2), Cyberlindnera fabianii (Cfab_RF37), Hyphopichia burtonii (Hbur_RF19), Mucor circinelloides (Mcir_RF48), Mucor indicus (Mind_RF25), Penicillium citrinum (Pcit_RF32), Rhodosporidiobolus ruineniae (Rrui_RF4 & Rrui_RF43), Saccharomyces cerevisiae (Scer_RF6), Saccharomycopsis fibuligera (Sfib_RF11), and Wickerhamomyces anomalus (Wano_RF3) among which the relative abundance (RA) of W. anomalus was the highest (24%) followed by C. glabrata and H. burtonii (16% in each). Five (Hbur_RF19, Sfib_RF11, Mind_RF25, Mcir_RF48, and Pcit_RF32) of eleven isolates showed amylase positive in the starch medium. Scer_RF6 showed the highest ethanol tolerance (14% v/v) followed by Hbur_RF19 (12% v/v), Cgla_RF2 (11% v/v) and Wano_RF3 (11% v/v). The amylase-positive strains produced beer-containing ethanol in the range of 3.17–7.3 (% v/v) from rice substrate. Although the rice beer produced by amylase-positive strains showed negligible pH difference, other parameters like ethanol, ascorbic acid, total phenol, and antioxidant properties were varied from beer to beer. Antibacterial activities shown by Mcir_RF48 and Pcit_RF32 against the test bacteria were higher with a 23–35 mm zone of inhibition than the other isolates. The present findings reveal the presence of fungi with antibacterial, amylolytic, ethanol fermenting, and antioxidant producing capacity in emao which could the source for future bioprospection.
Intraoperative Colonic Irrigation for Low Rectal Resections With Primary Anastomosis: A Fail-Safe Surgical Model
Authors: Herzberg Jonas , Khadem Shahram , Guraya Salman Yousuf , Strate Tim , Honarpisheh Human
Published: April 2022

Aim

Regardless the technological developments in surgery, the anastomotic leakage (AL) rate of low rectal anastomosis remains high. Though various perioperative protocols have been tested to reduce the risk for AL, there is no standard peri-operative management approach in rectal surgery. We aim to assess the short-term outcome of a multidisciplinary approach to reduce the rates of ALs using a fail-safe-model using preoperative and intraoperative colonic irrigation in low rectal resections with primary anastomosis.

Methods

Between January 2015 and December 2020, 92 patients received low rectal resections for rectal cancer with primary anastomosis and diverting ileostomy. All these patients received pre-operative mechanical bowel preparation (MBP) without antibiotics as well as intraoperative colonic irrigation. The intraoperative colonic irrigation was performed via the efferent loop of the ileostomy. All data were analyzed by SPSS for descriptive and inferential analyses.

Results

In the study period, 1.987 colorectal surgical procedures were performed. This study reports AL in 3 (3.3%) of 92 recruited patients. Other postoperative complications (Dindo-Clavien I-IV) were reported in 25 patients (27.2%), which occurred mainly due to non-surgical reasons such as renal dysfunction and sepsis. According to the fail-safe model, AL was treated by endoscopic or re-do surgery. The median postoperative length of hospitalization was 8 days (4–45) days.

Conclusion

This study validates the effectiveness of a multi-disciplinary fail-safe model with a pre-operative MBP and an intraoperative colonic irrigation in reducing AL rates. Intraoperative colonic irrigation is a feasible approach that lowers the AL rates by reducing fecal load and by decontamination of the colon and anastomotic region. Our study does not recommend a pre-operative administration of oral antibiotics for colorectal decontamination.
Current Knowledge on Infectious Bronchitis Virus Non-structural Proteins: The Bearer for Achieving Immune Evasion Function
Authors: Peng Shuwei , Wang Yiming , Zhang Yu , Song Xu , Zou Yuanfeng , Li Lixia , Zhao Xinghong , Yin Zhongqiong
Published: April 2022

Infectious bronchitis virus (IBV) is the first coronavirus discovered in the world, which is also the prototype of gamma-coronaviruses. Nowadays, IBV is widespread all over the world and has become one of the causative agent causing severe economic losses in poultry industry. Generally, it is believed that the viral replication and immune evasion functions of IBV were modulated by non-structural and accessory proteins, which were also considered as the causes for its pathogenicity. In this study, we summarized the current knowledge about the immune evasion functions of IBV non-structural and accessory proteins. Some non-structural proteins such as nsp2, nsp3, and nsp15 have been shown to antagonize the host innate immune response. Also, nsp7 and nsp16 can block the antigen presentation to inhibit the adapted immune response. In addition, nsp13, nsp14, and nsp16 are participating in the formation of viral mRNA cap to limit the recognition by innate immune system. In conclusion, it is of vital importance to understand the immune evasion functions of IBV non-structural and accessory proteins, which could help us to further explore the pathogenesis of IBV and provide new horizons for the prevention and treatment of IBV in the future.
Phytochemical Constituents of Propolis Flavonoid, Immunological Enhancement, and Anti-porcine Parvovirus Activities Isolated From Propolis
Authors: Ma Xia , Guo ZhenHuan , Li Yana , Yang Kun , Li Xianghui , Liu Yonglu , Shen Zhiqiang , Zhao Li , Zhang Zhiqiang
Published: April 2022

Propolis is widely used in health preservation and disease healing; it contains many ingredients. The previous study had revealed that the ethanolic or water extracts of propolis have a wide range of efficacy, such as antiviral, immune enhancement, anti-inflammatory, and so on, but its antiviral components and underlying mechanism of action remain unknown. In this study, we investigated the chemical composition, anti-porcine parvovirus (PPV) effectiveness, and immunological enhancement of propolis flavone ethanolic extracts. The chemical composition of propolis flavone was distinguished by ultra-performance liquid chromatography-quadrupole/time-of-flight tandem mass spectrometry analysis. In this study, the presence and characterization of 26 major components were distinguished in negative ionization modes to evaluate the effects of propolis flavonoid used as an adjuvant on the immune response of Landrace–Yorkshire hybrid sows immunized with an inactivated vaccine of PPV. Thirty Landrace-Yorkshire hybrid sows were randomly assigned to one of three groups, and the sows in the adjuvant groups were intramuscularly injected with PPV vaccine with a 2.0-ml propolis flavonoid adjuvant (PA) and oil emulsion adjuvant. After that, serum hemagglutination inhibition antibody titers and specific immunoglobulin (Ig)M and IgG subclasses were measured to evaluate the adjuvant effects of propolis flavonoid on the humoral immune responses, as well as peripheral lymphocyte proliferation activity and serum concentrations of Th1 and Th2 cytokines for cellular immunity. Results indicated an enhancing effect of PA on IgM, interleukins 2 and 4, interferon-γ, and IgG subclass responses. Especially in the effect of improving cellular immune response, the PA was the best. These findings suggested that PA can significantly enhance the immune responses against the PPV vaccine and could be an alternative way to improve PPV vaccination in sows. Furthermore, we screened the PF chemical components to the effectiveness of anti-PPV. Ferulic acid has an excellent anti-PPV effect.
Internet Survey Evaluation of Demographic Risk Factors for Injury in Canine Agility Athletes
Authors: Sundby Annika E. , Pechette Markley Arielle , Shoben Abigail B. , Kieves Nina R.
Published: April 2022

Objective

The purpose of this study was to compare previously identified demographic risk factors for injury in agility dogs, and explore other potential associations with demographic risk factors in new populations, and across different levels of injury severity.

Procedures

An internet-based survey of agility handlers was conducted. The primary outcome was if the dog had ever had an injury that kept from agility for over a week. Demographic information about the dog and handler were recorded. Logistic regression was used to quantify associations between variables of interest with injury history and all models were adjusted for age. Analyses were stratified by geographic location. Final model building was done via backward selection.

Results

The sample included 2,962 dogs from North America and 1,235 dogs from elsewhere. In the North American sample, 8 variables were associated with injury history; dog breed, height and weight, handler age, gender, agility experience, competing at the national level, age dog was acquired, and taking radiographs to assess growth plate closure. In the non-North American sample, 4 variables were associated with injury history; breed, handler age, occupation (dog trainer or not), and handler medical training. In both samples, Border Collies showed a marked increase in injury risk (ORs 1.89 and 2.34) and handler age >65 was associated with lower risk (ORs 0.62 and 0.77). Consistent with previous studies, greater handler experience was associated with reduced risk in the North American sample, but the other sample did not show this pattern, even in unadjusted models. Dog spay/neuter status was not associated with injury risk in either sample.

Conclusions and Clinical Relevance

Dogs with radiographs assessing growth plate closure may have increased injury risk as this population of owners may plan to train their dog harder, and at an earlier age. This finding also poses the question of whether or not growth plate closure is a good indicator of safety for increasing training intensity. Knowledge of what risk factors exist for injury in agility dogs is imperative in determining direction for future prospective studies, as well as creating recommendations to help prevent injury in this population of dogs.
Advanced Characterization of Monofloral Honeys from Romania
Authors: Pauliuc Daniela , Dranca Florina , Ropciuc Sorina , Oroian Mircea
Published: April 2022

Honey’s authenticity is a major concern for producers and consumers, and this prompts research into reliable methods to determine the source of honey (botanical and geographical). This study aimed to find the botanical origin of seven samples of monofloral honey (acacia, thyme, tilia, rape, raspberry, mint and sunflower) based on pollen analysis and identification of the physicochemical characteristics of these types of honey. For these types of honey, the following parameters were determined: color, electrical conductivity, free acidity, moisture content, pH, hydroxymethylfurfural content and sugar content. Alongside pollen analysis, these methods succeeded in classifying the analyzed samples as monofloral honey. Non-destructive methods of analysis such as Fourier transform infrared spectroscopy, the determination of the rheological behavior of honey in the negative domain and the determination of the thermal behavior were also employed to characterize the honey samples. The best differentiation between samples was achieved in the spectral region between 950 and 750 cm−1, which is of interest for carbohydrate analysis in IR spectroscopy. In the negative interval, the viscous and elastic modules intersected at different temperatures, these temperatures being influenced both by the moisture of the analyzed samples and the botanical origin.
Effects of Multi-Species Microbial Inoculants on Early Wheat Growth and Litterbag Microbial Activity
Authors: Hett Jonas , Neuhoff Daniel , Döring Thomas F. , Masoero Giorgio , Ercole Enrico , Bevivino Annamaria
Published: April 2022

The use of microbial consortia (MC) with complementing features is considered to be a promising method of sustainable crop intensification, potentially trumping the limited performance of single-strain applications. We assessed the effect of two novel MC on early wheat growth and litterbag microbial activity in heated and unheated soil. Pot experiments were carried out in duplicate in a greenhouse over 63 days using a completely randomized design with six replications. A range of parameters of plant growth and nutrient uptake were regularly assessed and statistically analyzed by ANOVA. The litterbag-NIRS method was used to trace the microbial activity. Averaged over both trials, soil heating resulted in a significant increase in shoot biomass (+53%) and subsequent nitrogen uptake (+307 mg N pot−1) but strongly reduced root development (−46%) compared with unheated soil. The application of MC had no effect on wheat growth in the heated soil. By contrast, in the unheated soil, shoot (+12%) and root (+15%) biomass and shoot nitrogen uptake (+11%) were significantly increased after double inoculation with MC compared with autoclaved MC. The litterbag-NIRS method confirmed clear effects of soil heating on microbial activity. Differences between MC application and the control were noted, indicating a buffering effect of MC.