bluuebunny/crossref_metadata_2025 huggingface dataset:
Experimental Investigation of Lateral Load Test on Diagonal Braced 3M Glass Fiber Reinforced Polymer Transmission Tower
Authors: Krishnasamy R. , Johnson S. Christian , Kumar P. Saravana , Mohanraj R.
Published: March 2024

Over the past decades, numerous research and development studies worldwide have focused on various civil engineering applications involving composite materials. Presently, recent investigations have explored the utilization of Glass Fiber Reinforced Polymer (GFRP) members in tower panels, composite cross arms in towers, full-scale 66 kV FRP double circuit towers, FRP towers created through filament winding techniques, GFRP triangular base communication towers, GFRP square base tower panels with bolted connections and strength and stability assessments of Glass Fiber Polyamide (GFP) towers and carbon fibre reinforced towers. The susceptibility of conventional TLT members to theft, where thieves often cut and steal them, prompted the suggestion to consider TLTs constructed with different materials. The study for examining the behavior of hybrid joints in angle-to-angle connections, with an ‘e/d’ ratio is 5. The well-performing hybrid joint has been implemented in a GFRP tower model and subjected to lateral loading to assess the effectiveness of such joints.
Comparison of three-dimensional cell culture techniques of dedifferentiated liposarcoma and their integration with future research
Authors: Tahara Sayumi , Sharma Soumya , de Faria Fernanda Costas Casal , Sarchet Patricia , Tomasello Luisa , Rentsch Sydney , Karna Roma , Calore Federica , Pollock Raphael E.
Published: March 2024

Background: Dedifferentiated liposarcoma is a formidable sarcoma subtype due to its high local recurrence rate and resistance to medical treatment. While 2D cell cultures are still commonly used, 3D cell culture systems have emerged as a promising alternative, particularly scaffold-based techniques that enable the creation of 3D models with more accurate cell-stroma interactions.

Objective: To investigate how 3D structures with or without the scaffold existence would affect liposarcoma cell lines growth morphologically and biologically.

Methods: Lipo246 and Lipo863 cell lines were cultured in 3D using four different methods; Matrigel® ECM scaffold method, Collagen ECM scaffold method, ULA plate method and Hanging drop method, in addition to conventional 2D cell culture methods. All samples were processed for histopathological analysis (HE, IHC and DNAscope™), Western blot, and qPCR; moreover, 3D collagen-based models were treated with different doses of SAR405838, a well-known inhibitor of MDM2, and cell viability was assessed in comparison to 2D model drug response.

Results: Regarding morphology, cell lines behaved differently comparing the scaffold-based and scaffold-free methods. Lipo863 formed spheroids in Matrigel® but not in collagen, while Lipo246 did not form spheroids in either collagen or Matrigel®. On the other hand, both cell lines formed spheroids using scaffold-free methods. All samples retained liposarcoma characteristic, such as high level of MDM2 protein expression and MDM2 DNA amplification after being cultivated in 3D. 3D collagen samples showed higher cell viability after SAR40538 treatment than 2D models, while cells sensitive to the drug died by apoptosis or necrosis.

Conclusion: Our results prompt us to extend our investigation by applying our 3D models to further oncological relevant applications, which may help address unresolved questions about dedifferentiated liposarcoma biology.
Ionic liquid electrolyte selection for high voltage supercapacitors in high-temperature applications
Authors: Bahaa Ahmed , Alhammadi Ayoob , Lethesh Kallidanthiyil Chellappan , Susantyoko Rahmat Agung , Bamgbopa Musbaudeen O.
Published: March 2024

Systematic analyses of electrolyte physicochemical properties are important to screen ionic liquids (ILs) and understand the electrochemical performance of supercapacitor electrolytes. This study harmonizes the evaluation of electrochemical performance and transport properties of eight shortlisted ILs from 22 commercially available hydrophobic ILs toward achieving a ≥ 5 V supercapacitor capable of high-temperature operation (up to 353.15 K). The eight ILs are N-Propyl-N-methylpyrrolidinium bis(trifluoromethanesulfonyl)imide ([Pyr 1, 3] [TFSI], N-Pentyl-N-methylpyrrolidinium bis(trifluoromethanesulfonyl)imide ([Pyr 1, 5] [TFSI]), N-Propyl-N-methylpyrrolidinium bis(fluorosulfonyl)imide ([Pyr 1, 3] [FSI]), 1-Methyl-1-(2-methoxyethyl)pyrrolidinium Bis(trifluoromethanesulfonyl)imide ([Pyr 1, 102] [TFSI]), 1-Methyl-1-propylpiperidinium bis(trifluoromethanesulfonyl)imide ([Pip 1, 3] [TFSI]), 1-Methyl-1-propylpiperidinium bis(fluorosulfonyl)imide ([Pip 1, 3] [FSI]), N-Trimethyl-N-propylammonium bis(trifluoromethanesulfonyl)imide ([N 111, 3] [TFSI]), N-Trimethyl-N-hexylammonium bis(trifluoromethanesulfonyl)imide ([N 111, 6] [TFSI]). The density, viscosity, and ionic conductivity of the eight ILs were measured between 278.15 and 373.15 K to confirm the effects of temperature and ion structure before electrochemical characterization. The [FSI]-based ILs ([Pip 1, 3] [FSI] and [Pyr 1, 3] [FSI]) showed lower densities and viscosities compared to other ILs among the eight based on [TFSI]. Consequently, the highest conductivity was obtained for [Pyr 1, 3] [FSI]. Cyclic voltammetry and impedance spectroscopy was performed on supercapacitors assembled with the eight ILs as electrolytes between 298.15–353.15 K. Conclusion from the two-electrode supercapacitors using multi-walled carbon nanotubes showed the 6 most-applicable ILs towards the targeted ≥ 5 V SC at high temperature are [Pip 1, 3] [TFSI] (5.4 V), [Pip 1, 3] [FSI] (5 V), [N 111, 3] [TFSI] (5.1 V), [N 111, 6] [TFSI] (5.2 V), [Pyr 1, 102] [TFSI] (5.2 V), and [Pyr 1, 5] [TFSI] (5.2 V).
Development and validation a nomogram prediction model for early diagnosis of bloodstream infections in the intensive care unit
Authors: Qi Zhili , Dong Lei , Lin Jin , Duan Meili
Published: March 2024

Purpose

This study aims to develop and validate a nomogram for predicting the risk of bloodstream infections (BSI) in critically ill patients based on their admission status to the Intensive Care Unit (ICU).

Patients and methods

Patients’ data were extracted from the Medical Information Mart for Intensive Care−IV (MIMIC−IV) database (training set), the Beijing Friendship Hospital (BFH) database (validation set) and the eICU Collaborative Research Database (eICU−CRD) (validation set). Univariate logistic regression analyses were used to analyze the influencing factors, and lasso regression was used to select the predictive factors. Model performance was assessed using area under receiver operating characteristic curve (AUROC) and Presented as a Nomogram. Various aspects of the established predictive nomogram were evaluated, including discrimination, calibration, and clinical utility.

Results

The model dataset consisted of 14930 patients (1444 BSI patients) from the MIMIC-IV database, divided into the training and internal validation datasets in a 7:3 ratio. The eICU dataset included 2100 patients (100 with BSI) as the eICU validation dataset, and the BFH dataset included 419 patients (21 with BSI) as the BFH validation dataset. The nomogram was constructed based on Glasgow Coma Scale (GCS), sepsis related organ failure assessment (SOFA) score, temperature, heart rate, respiratory rate, white blood cell (WBC), red width of distribution (RDW), renal replacement therapy and presence of liver disease on their admission status to the ICU. The AUROCs were 0.83 (CI 95%:0.81-0.84) in the training dataset, 0.88 (CI 95%:0.88-0.96) in the BFH validation dataset, and 0.75 (95%CI 0.70-0.79) in the eICU validation dataset. The clinical effect curve and decision curve showed that most areas of the decision curve of this model were greater than 0, indicating that this model has a certain clinical effectiveness.

Conclusion

The nomogram developed in this study provides a valuable tool for clinicians and nurses to assess individual risk, enabling them to identify patients at a high risk of bloodstream infections in the ICU.
Novel evidence for cue-based retrieval of top-down sets in spatial cueing
Authors: Büsel Christian , Valuch Christian , Schmid Rebecca Rosa , Sachse Pierre , Ansorge Ulrich
Published: March 2024

Task cues that correctly (vs.incorrectly) inform humans about their upcoming tasks, benefit (vs. interfere with) performance because participants can use task cues to retrieve the corresponding task set, so that targets can (vs. cannot) be processed according to the currently applying task set from target onset onwards. Here, we tested if task-associated features of peripheral cues have a similar effect. Typically, peripheral cues with a task-associated, searched-for color (i.e., top-down matching cues) capture attention: Search for targets presented at the cued position (valid condition) is faster than for targets presented away from the cue (invalid condition), even if cues do not predict the likely target location. For example, when searching for red and green targets, a red cue captures attention even if presented prior to a green target, but a blue cue does not. We know that cue-target color congruence—whether the cue has a target-similar color (congruent case) vs. a target-dissimilar color (incongruent case)—additionally expedites vs. delays search times. However, it is unclear if this congruence effect reflects feed-forward color priming of the target only; or if cue-elicited retrieval of color-specific task sets is involved. Crucially, we hypothesized that cue-based task-set retrieval should incur additional costs if the task sets for the two target colors differ more. In contrast, mere feed-forward priming should not be affected by task-set similarity between color-associated task sets. Congruence effects were indeed larger when color-associated task sets were more different. This finding indicates cue-elicited retrieval of color-associated task sets can contribute to effects of cue-target color congruence. Results are discussed in light of recent theories.
Evaluation on the willingness of migrants and native farmers to transfer farmland in resettlement areas and their coupled coordination relationships: perspectives for environmental and social sustainability
Authors: Chen Shaojun , Yu Danyang , Lei Ming , Sun Jie , Wang Xin
Published: March 2024

The construction of water conservancy and hydropower projects often involves substantial land acquisition and resettlement. Land is a crucial means of production, playing a significant role in ensuring food security and protecting the ecological environment and social development. However, the advancement of urbanization and changes in production methods have led to a noticeable shift in the behavior of farmers on lands. Abandoned land and reclaimed wasteland in host resettlement areas lead to low land-use efficiency, environmental degradation, and frequent geological disasters. It is important to evaluate the willingness of migrants and native farmers to transfer farmland in the resettlement area and their coupled coordination relationships to realize effective land use. These were evaluated in this study by constructing an index system from personal and family characteristics, land resource endowment, characteristics of the external policy environment, and psycho–cognitive characteristics. The results showed that their coupling degree was 0.999, while their coupling degree of coordination was 0.68. The relative development degree was 1.01. It showed that a strong correlation existed between the willingness of migrants and native farmers, and they were in a basic coordination phase. They influenced each other and reached a basic balanced state. Farmland can be transferred between migrants and native farmers to improve their livelihood, resettle migrants, improve land-use efficiency, and reduce wasteland reclamation. This suggested the possibility of establishing a land transfer resettlement model based on the willingness of migrants and native farmers to achieve environmental and social sustainability in the resettlement area.
Role of circular RNAs in lung cancer
Authors: Babayev Maksat , Silveyra Patricia
Published: March 2024

Lung cancer remains a global public health concern with significant research focus on developing better diagnosis/prognosis biomarkers and therapeutical targets. Circular RNAs (circRNAs) are a type of single-stranded RNA molecules that covalently closed and have ubiquitous expression. These molecules have been implicated in a variety of disease mechanisms, including lung cancer, as they exhibit oncogenic or tumor suppressor characteristics. Recent research has shown an important role that circRNAs play at different stages of lung cancer, particularly in lung adenocarcinoma. In this review, we summarize the latest research on circRNAs and their roles within lung cancer diagnosis, as well as on disease mechanisms. We also discuss the knowledge gaps on these topics and possible future research directions.
Targeting tumor-infiltrating tregs for improved antitumor responses
Authors: Qin Diyuan , Zhang Yugu , Shu Pei , Lei Yanna , Li Xiaoyu , Wang Yongsheng
Published: March 2024

Immunotherapies have revolutionized the landscape of cancer treatment. Regulatory T cells (Tregs), as crucial components of the tumor immune environment, has great therapeutic potential. However, nonspecific inhibition of Tregs in therapies may not lead to enhanced antitumor responses, but could also trigger autoimmune reactions in patients, resulting in intolerable treatment side effects. Hence, the precision targeting and inhibition of tumor-infiltrating Tregs is of paramount importance. In this overview, we summarize the characteristics and subpopulations of Tregs within tumor microenvironment and their inhibitory mechanisms in antitumor responses. Furthermore, we discuss the current major strategies targeting regulatory T cells, weighing their advantages and limitations, and summarize representative clinical trials targeting Tregs in cancer treatment. We believe that developing therapies that specifically target and suppress tumor-infiltrating Tregs holds great promise for advancing immune-based therapies.
Effects of tumor necrosis factor-alpha inhibitors on lipid profiles in patients with psoriasis: a systematic review and meta-analysis
Authors: Su Liang , Xu Chunyan , Huang Hong , Zhang Peilian , Wang Jinrong , Ouyang Xiaoyong , Yang Xuesong , Ye Jianzhou
Published: March 2024

Background

There is no consensus on the effect of tumor necrosis factor-alpha (TNF-alpha) inhibitors on lipid profiles in patients with psoriasis. This study aimed to investigate the effects of TNF-alpha inhibitors on lipid profiles (triglycerides, total cholesterol, low-density lipoprotein, or high-density lipoprotein) in patients with psoriasis.

Methods

We searched PubMed, Embase, and Cochrane Library databases for articles published before October 17, 2023. Four TNF-alpha inhibitors (infliximab, etanercept, adalimumab, and certolizumab) were included in our study. (PROSPERO ID: CRD42023469703).

Results

A total of twenty trials were included. Overall results revealed that TNF-alpha inhibitors elevated high-density lipoprotein levels in patients with psoriasis (WMD = 2.31; 95% CI: 0.96, 3.67; P = 0.001), which was supported by the results of sensitivity analyses excluding the effect of lipid-lowering drugs. Subgroup analyses indicated that high-density lipoprotein levels were significantly increased in the less than or equal to 3 months group (WMD = 2.88; 95% CI: 1.37, 4.4; P < 0.001), the etanercept group (WMD = 3.4; 95% CI = 1.71, 5.09, P < 0.001), and the psoriasis group (WMD = 2.52; 95% CI = 0.57, 4.48, P = 0.011). Triglyceride levels were significantly increased in the 3 to 6-month group (WMD = 4.98; 95% CI = 1.97, 7.99, P = 0.001) and significantly decreased in the 6-month and older group (WMD = -19.84; 95% CI = -23.97, -15.7, P < 0.001). Additionally, Triglyceride levels were significantly increased in the psoriasis group (WMD = 5.22; 95% CI = 2.23, 8.21, P = 0.001).

Conclusion

Our results revealed that TNF-alpha inhibitors might temporarily increase high-density lipoprotein levels in patients with psoriasis. However, changes in triglycerides were not consistent among the different durations of treatment, with significant increases after 3 to 6 months of treatment. Future prospective trials with long-term follow-up contribute to confirming and extending our findings.

Systematic Review Registration

https://www.crd.york.ac.uk/PROSPERO/, identifier CRD42023469703.
Health-related quality of life among celiacs in Portugal: a comparison between general and specific questionnaires
Authors: Chaves Cláudia , Zandonadi Renata Puppin , Raposo António , Nakano Eduardo Yoshio , Ramos Fernando , Farage Priscila , Teixeira-Lemos Edite
Published: March 2024

Objective

This study aimed to compare the 36-Item Short Form Survey Instrument version 2 (SF-36-v2) (generic) and Celiac Disease Questionnaire (CDQ) (specific) questionnaires used to evaluate the quality of life (QoL) in celiac Portuguese adult individuals.

Methods

This cross-sectional study used non-probabilistic sampling based on Portuguese celiac patients who accessed the online survey in 2022. The online data collection used a self-reported instrument composed of three parts: (i) socioeconomic, health, and gluten-free diet (GFD) adherence questions; (ii) SF-36 v2 – Portuguese version (generic questionnaire) and (iii) Celiac Disease Questionnaire (CDQ) (specific questionnaire).

Results

A total of 234 individuals who accessed the survey completed the questionnaire. Seven of the eight SF-36 domains positively correlated to the specific questionnaire CDQ. The “General Health” domain (domain 4) showed a negative correlation with the CDQ. Differences in content between the two instruments might be able to explain this finding since the CDQ explores issues regarding the specificities of celiac disease (CD) and the lifelong GFD burden. About half of the sample from this study displayed poor diet adherence, it is possible that the SF-36 could not reflect the impact of CD treatment - the complete elimination of gluten from the diet - on patients’ health. Therefore, this issue should be carefully evaluated in future research.

Conclusion

Specific validated questionnaires for CD individuals, such as the CDQ, contemplate social, economic, and clinical variables that permeate the patient’s life context. Therefore, these instruments may be more suitable for evaluating QoL in this public. However, using a general questionnaire such as the SF-36 would be indicated for comparing QOL between celiac patients and the general population or even between CD and other disease individuals. In this case, we recommend assessing GFD compliance for control parallelly.
Bycatch mitigation from the sky: using helicopter communication for mobulid conservation in tropical tuna fisheries
Authors: Waldo Jennifer L. , Altamirano-Nieto Ernesto , Croll Donald A. , Palacios Marta D. , Lezama-Ochoa Nerea , Lopez Jon , Moreno Gala , Rojas-Perea Stefany , Cronin Melissa R.
Published: March 2024

The incidental capture of non-target species (bycatch) in tuna fisheries impacts some marine vertebrates, particularly species with vulnerable life histories such as manta and devil rays (mobulids). There is broad interest in reducing mobulid bycatch in tuna purse seine fisheries, with existing efforts mainly focusing on reducing post-capture mortality rates. We explore a novel potential pre-capture mobulid bycatch avoidance strategy for the tuna purse seine fishery using communication between fishing vessels and associated spotter helicopters. We conducted a survey of tuna purse seine helicopter pilots, spotters, and fishers operating in the eastern Pacific Ocean (n = 33) to ascertain the ability of helicopter crew to detect mobulids prior to capture and communicate bycatch avoidance with vessel crew. Results indicate over half of the helicopter crew report being “always” or “sometimes” able to sight and identify mobulids and that helicopter crew regularly communicate mobulid sightings to the vessel already. Given that an average of 63% of class-6 vessel trips between 2017 to 2022 carried onboard helicopters, our results suggest that helicopter-vessel communication could be feasible and scalable for mobulid bycatch detection, enabling potential bycatch avoidance and early alerts for proper handling protocols. We also identify the potential use of helicopter detection to improve research efforts for mobulid conservation (e.g., data collection of population and habitat observations). This study is the first to investigate the utility of helicopter-vessel communication as a bycatch mitigation strategy for elasmobranchs and identifies research and management directions that could be further investigated to avoid bycatch of mobulids.
Effect of PCI on ophthalmic artery hemodynamics in patients with acute coronary syndrome
Authors: Liu Wen-long , Wu Lan-ting , Wang Jia-lin , Sun Jiao , Cheng Xue-ru , Zhou Zhuo-hua , Guan Jia-xin , Wang Yan-ling , Meng Zhao-yang
Published: March 2024

Purpose

We aimed to explore the effects of percutaneous coronary intervention (PCI) on the ophthalmic artery (OA) hemodynamics in patients with acute coronary syndrome (ACS).

Methods

A total of 73 participants (Group0: healthy controls, Group1: Patients with ACS underwent PCI < 3 months, Group2: Patients with ACS underwent PCI ≥ 3 months) were enrolled. Computed tomographic angiography images were used to construct three-dimensional models of participants' OAs. Numerical simulations based on computational fluid dynamics were used to acquire hemodynamic parameters.

Results

The angle between the OA and internal carotid artery in Group2 was significantly larger compared with Group0 and Group1 (P = 0.003 and P = 0.044). Hemodynamic simulation showed a significantly slower OA blood velocity in Group1 than in the control (P < 0.001) and Group2 (P = 0.033). Lower wall shear stress was found in Group1 than that in control (P = 0.040). Patients after PCI had a higher wall pressure than healthy controls (P = 0.012 and P = 0.004). Mass flow ratios were decreased in Group1 and Group2 (P = 0.021 and P = 0.002). The hemodynamic parameters of OA were correlated with several clinical indicators.

Conclusions

The OA blood flow velocity of patients with ACS after PCI initially slowed down, which increased the risk of plaque formation, and then showed an increasing trend. There was a correlation between OA hemodynamic parameters and clinical indexes related to cardiac stress. Ischemia-reperfusion injury and changes in blood flow status after PCI may affect OA morphology and hemodynamics, leading to ocular lesions.

Trial registration

ChiCTR2100050428.
Normal distribution of H3K9me3 occupancy co-mediated by histone methyltransferase BcDIM5 and histone deacetylase BcHda1 maintains stable ABA synthesis in Botrytis cinerea TB-31
Authors: Wei Zhao , Shu Dan , Hou Xiaonan , Li Tianfu , Li Zhemin , Luo Di , Yang Jie , Tan Hong
Published: March 2024

Abscisic acid (ABA) is a conserved and important “sesquiterpene signaling molecule” widely distributed in different organisms with unique biological functions. ABA coordinates reciprocity and competition between microorganisms and their hosts. In addition, ABA also regulates immune and stress responses in plants and animals. Therefore, ABA has a wide range of applications in agriculture, medicine and related fields. The plant pathogenic ascomycete B. cinerea has been extensively studied as a model strain for ABA production. Nevertheless, there is a relative dearth of research regarding the regulatory mechanism governing ABA biosynthesis in B. cinerea. Here, we discovered that H3K9 methyltransferase BcDIM5 is physically associated with the H3K14 deacetylase BcHda1. Deletion of Bcdim5 and Bchda1 in the high ABA-producing B. cinerea TB-31 led to severe impairment of ABA synthesis. The combined analysis of RNA-seq and ChIP-seq has revealed that the absence of BcDIM5 and BcHda1 has resulted in significant global deficiencies in the normal distribution and level of H3K9me3 modification. In addition, we found that the cause of the decreased ABA production in the ΔBcdim5 and ΔBchda1 mutants was due to cluster gene repression caused by the emergence of hyper-H3K9me3 in the ABA gene cluster. We concluded that the ABA gene cluster is co-regulated by BcDIM5 and BcHda1, which are essential for the normal distribution of the B. cinerea TB-31 ABA gene cluster H3K9me3. This work expands our understanding of the complex regulatory network of ABA biosynthesis and provides a theoretical basis for genetic improvement of high-yielding ABA strains.
Dysfunction of cecal microbiota and CutC activity in mice mediating diarrhea with kidney-yang deficiency syndrome
Authors: Guo Mingmin , Fang Leyao , Chen Meili , Shen Junxi , Tan Zhoujin , He Wenzhi
Published: March 2024

Objective

Previous studies have indicated that diarrhea with kidney-yang deficiency syndrome leads to a disorder of small intestine contents and mucosal microbiota. However, the relationship of TMA-lyase (CutC) activity and TMAO with diarrhea with kidney-yang deficiency syndrome remains unexplored. Therefore, this study explores the relationship between cecal microbiota and choline TMA-lyase (CutC) activity, as well as the correlation between trimethylamine oxide (TMAO), inflammatory index, and CutC activity.

Method

Twenty SPF-grade male KM mice were randomly divided into the normal group (CN) and the diarrhea model group (CD). Diarrhea mouse models were established by adenine combined with Folium sennae administration. CutC activity, TMAO, interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) levels were detected, and the cecal content microbiota was sequenced.

Result

After 14 days, diarrhea occurred in the CD group. Compared with the CN group, there was no significant change in the activity of CutC in the small intestine of the CD group, while the activity of CutC in the cecum was significantly increased, and the levels of TMAO, IL-6, and TNF-α showed a significant increase. The Chao1 index, Observed_species index, Shannon index, and Simpson index all exhibited a decreasing trend. The main changes at the bacterial genus level were Alistipes, Enterorhabdus, Desulfovibrio, Bacteroides, Candidatus_Saccharimonas, and [Ruminococcus]_torques_group. The results of LEfSe analysis, random forest analysis and ROC curve analysis revealed Paludicola, Blautia, Negativibacillus, Paraprevotella, Harryflintia, Candidatus_Soleaferrea, Anaerotruncus, Oscillibacter, Colidextribacter, [Ruminococcus]_torques_group, and Bacteroides as characteristic bacteria in the CD group. Correlation analysis showed a significant negative correlation between cecal CutC activity and Ligilactobacillus, and a significant positive correlation with Negativibacillus and Paludicola. The level of TMAO was significantly positively correlated with CutC activity and IL-6.

Conclusion

Diarrhea with kidney-yang deficiency syndrome significantly affects the physiological status, digestive enzyme activity, CutC activity, TMAO levels, and inflammatory response in mice. Additionally, there are changes in the composition and function of cecal microbiota, indicating an important impact of diarrhea with kidney-yang deficiency syndrome on the host intestinal microbiota balance. The occurrence of diarrhea with kidney-yang deficiency syndrome may be associated with dysbiosis of intestinal microbiota, increased CutC activity, elevated TMAO levels, and heightened inflammatory factor levels.
Comparison of local activation, functional connectivity, and structural connectivity in the N-back task
Authors: Satake Takatoshi , Taki Ai , Kasahara Kazumi , Yoshimaru Daisuke , Tsurugizawa Tomokazu
Published: March 2024

The N-back task is widely used to investigate working memory. Previous functional magnetic resonance imaging (fMRI) studies have shown that local brain activation depends on the difficulty of the N-back task. Recently, changes in functional connectivity and local activation during a task, such as a single-hand movement task, have been reported to give the distinct information. However, previous studies have not investigated functional connectivity changes in the entire brain during N-back tasks. In this study, we compared alterations in functional connectivity and local activation related to the difficulty of the N-back task. Because structural connectivity has been reported to be associated with local activation, we also investigated the relationship between structural connectivity and accuracy in a N-back task using diffusion tensor imaging (DTI). Changes in functional connectivity depend on the difficulty of the N-back task in a manner different from local activation, and the 2-back task is the best method for investigating working memory. This indicates that local activation and functional connectivity reflect different neuronal events during the N-back task. The top 10 structural connectivities associated with accuracy in the 2-back task were locally activated during the 2-back task. Therefore, structural connectivity as well as fMRI will be useful for predicting the accuracy of the 2-back task.
Upper extremity training followed by lower extremity training with a brain-computer interface rehabilitation system
Authors: Sieghartsleitner Sebastian , Sebastián-Romagosa Marc , Cho Woosang , Grünwald Johannes , Ortner Rupert , Scharinger Josef , Kamada Kyousuke , Guger Christoph
Published: March 2024

Introduction

Brain-computer interfaces (BCIs) based on functional electrical stimulation have been used for upper extremity motor rehabilitation after stroke. However, little is known about their efficacy for multiple BCI treatments. In this study, 19 stroke patients participated in 25 upper extremity followed by 25 lower extremity BCI training sessions.

Methods

Patients’ functional state was assessed using two sets of clinical scales for the two BCI treatments. The Upper Extremity Fugl-Meyer Assessment (FMA-UE) and the 10-Meter Walk Test (10MWT) were the primary outcome measures for the upper and lower extremity BCI treatments, respectively.

Results

Patients’ motor function as assessed by the FMA-UE improved by an average of 4.2 points (p < 0.001) following upper extremity BCI treatment. In addition, improvements in activities of daily living and clinically relevant improvements in hand and finger spasticity were observed. Patients showed further improvements after the lower extremity BCI treatment, with walking speed as measured by the 10MWT increasing by 0.15 m/s (p = 0.001), reflecting a substantial meaningful change. Furthermore, a clinically relevant improvement in ankle spasticity and balance and mobility were observed.

Discussion

The results of the current study provide evidence that both upper and lower extremity BCI treatments, as well as their combination, are effective in facilitating functional improvements after stroke. In addition, and most importantly improvements did not stop after the first 25 upper extremity BCI sessions.
Caucasian and African American racial disparity in neonatal hospital mortality
Authors: Burdjalov Maria , Qattea Ibrahim , Nandakumar Vanishree , Mohamed Mohamed A. , Aly Hany
Published: March 2024

Objective

To examine disparity in hospital mortality among Caucasian (C) and African American (AA) neonates born at different gestational ages (GA).

Methods

De-identified national inpatient data were obtained from the Healthcare Cost and Utilization Project (HCUP) from the Agency for Healthcare Research and Quality (AHRQ) for the years 2011–2018. We compared the odds ratio for mortality among C and AA infants by sex and GA category. Analyses were repeated after controlling for multiple maternal and neonatal confounding variables in a logistic regression model.

Results

The study included 18,758,233 infants; 78.3% of them were C and 21.7% were AA. Compared to C population, AA population has a significantly higher mortality in term infants born at GA ≥ 36 weeks. The racial/ethnic disparity in preterm infants was inconsistent without any difference at 35–36 weeks in male and female infants. The overall aOR for mortality in AA in all male preterm infants ≤36 weeks was 1.44 (1.39–1.49), <0.01; and the overall aOR for mortality in AA in all preterm female infants ≤36 weeks was 1.38 (1.33–1.44).

Conclusion

Racial/Ethnic disparity in hospital mortality exists with higher AA mortality in infants born with GA > 36 weeks and less AA mortality in infants born with GA 24–26 weeks.
Synthesis, biological and computational evaluation of novel cyanomethyl vinyl ether derivatives
Authors: Martín-Encinas Endika , Fuertes María , Delgado-Hernández Samuel , García-Tellado Fernando , Tejedor David , Alonso Concepción
Published: March 2024

This work explores the biological evaluation of novel cyanomethyl vinyl ether derivatives as antiproliferative agents. Tubulin, crucial to microtubule structure and function, is a target for cancer therapies. In vitro cytotoxicity assessments revealed significant activity in SKOV3 ovarian carcinoma cells and A549 lung carcinoma cells. Structure-Activity Relationship (SAR) analysis indicated that the E isomer and specific substitutions influenced the biological activity. Computational assays predicted favorable ADME properties, highlighting potential as anticancerous agents. Molecular docking studies demonstrated that compound 12E, with the E geometry of the double bond and fused polyaromatic rings such as phenanthrene, has robust interaction with tubulin, suggesting enhanced stability due to diverse amino acid interactions. Comparative spatial distributions with colchicine further indicated potential mechanistic similarities.
Effect of aromatic substituents on thermoresponsive functional polycaprolactone micellar carriers for doxorubicin delivery
Authors: Wang Hanghang , Polara Himanshu , Bhadran Abhi , Shah Tejas , Babanyinah Godwin Kweku , Ma Ziyuan , Calubaquib Erika L. , Miller Justin T. , Biewer Michael C. , Stefan Mihaela C.
Published: March 2024

Amphiphilic functional polycaprolactone (PCL) diblock copolymers are excellent candidates for micellar drug delivery. The functional groups on the backbone significantly affect the properties of PCL. A systematic investigation of the effect of aromatic substituents on the self-assembly of γ-functionalized PCLs and the delivery of doxorubicin (DOX) is presented in this work. Three thermoresponsive amphiphilic diblock copolymers with poly(γ-benzyloxy-ε-caprolactone) (PBnCL), poly(γ-phenyl- ε-caprolactone) (PPhCL), poly(γ-(4-ethoxyphenyl)-ε-caprolactone) (PEtOPhCL), respectively, as hydrophobic block and γ-tri(ethylene glycol) functionalized PCL (PME3CL) as hydrophilic block were prepared through ring-opening polymerization (ROP). The thermoresponsivity, thermodynamic stability, micelle size, morphology, DOX-loading, and release profile were determined. The LCST values of amphiphilic diblock copolymers PME3CL-b-PBnCL, PME3CL-b-PPhCL, and PME3CL-b-PEtOPhCL are 74.2°C, 43.3°C, and 37.3°C, respectively. All three copolymers formed spherical micelles in phosphate-buffered saline (PBS, 1×, pH = 7.4) at low concentrations ranging from 8.7 × 10−4 g/L to 8.9 × 10−4 g/L. PME3CL-b-PBnCL micelles showed the highest DOX loading capacity of 3.01 ± 0.18 (wt%) and the lowest drug release, while PME3CL-b-PEtOPhCL micelles exhibited the lowest DOX loading capacity of 1.95 ± 0.05 (wt%) and the highest drug release. Cytotoxicity and cellular uptake of all three micelles were assessed in vitro using MDA-MB-231 breast cancer cells. All three empty micelles did not show significant toxicity to the cells at concentrations high up to 0.5 mg/mL. All three DOX-loaded micelles were uptaken into the cells, and DOX was internalized into the nucleus of the cells.
In vitro biological control of Pyrrhoderma noxium using volatile compounds produced by termite gut-associated streptomycetes
Authors: Adra Cherrihan , Panchalingam Harrchun , Foster Keith , Tomlin Russell , Hayes R. Andrew , Kurtböke D. İpek
Published: March 2024

Introduction

Pyrrhoderma noxium is a plant pathogen that causes economic losses in agricultural and forestry industries, including significant destruction to amenity trees within the city of Brisbane in Australia. Use of chemical control agents are restricted in public areas, there is therefore an urgent need to investigate biological control approaches. Members of the phylum Actinomycetota, commonly known as actinomycetes, are known for their industrially important secondary metabolites including antifungal agents. They have proven to be ideal candidates to produce environmentally friendly compounds including the volatile organic compounds (VOCs) which can be used as biofumigants.

Methods

Different Streptomyces species (n=15) previously isolated from the guts of termites and stored in the University of the Sunshine Coast’sMicrobial Library were tested for their antifungal VOCs against Pyrrhoderma noxium.

Results

Fourteen of them were found to display inhibition (39.39-100%) to the mycelial development of the pathogen. Strongest antifungal activity displaying isolates USC-592, USC-595, USC-6910 and USC-6928 against the pathogen were selected for further investigations. Their VOCs were also found to have plant growth promotional activity observed for Arabidopsis thaliana with an increase of root length (22-36%) and shoot length (26-57%). The chlorophyll content of the test plant had a slight increase of 11.8% as well. Identified VOCs included geosmin, 2-methylisoborneol, 2-methylbutyrate, methylene cyclopentane, β-pinene, dimethyl disulfide, ethyl isovalerate, methoxyphenyl-oxime and α-pinene. Additionally, all 15 Streptomyces isolates were found to produce siderophores and indole acetic acid as well as the enzyme chitinase which is known to break down the fungal cell wall.

Discussion

Findings indicate that termite gut-associated streptomycetes might be used to control Pyrrhoderma noxium by utilizing their wide range of inhibitory mechanisms.
Satisfaction, engagement, and outcomes in internet-delivered cognitive behaviour therapy adapted for people of diverse ethnocultural groups: an observational trial with benchmarking
Authors: Sapkota Ram P. , Valli Emma , Dear Blake F. , Titov Nickolai , Hadjistavropoulos Heather D.
Published: March 2024

Introduction

Depression and anxiety are the most common mental health disorders worldwide. Internet-Delivered Cognitive Behaviour Therapy (ICBT) can reduce barriers to care to broad cross sections of the population. However, People of Diverse Ethnocultural Backgrounds (PDEGs) other than White/Caucasian underutilize mental health services and are under represented in clinical trials of psychological interventions.

Methods

To address this research gap we adapted an evidence-based ICBT program for PDEGs. The current pilot study explores the engagement, satisfaction, and effectiveness in the adapted ICBT program by PDEGs (N=41) when benchmarked against a sample of PDEGs (N=134) who previously completed a non-adapted version of the ICBT program.

Results

An intent-to-treat analyses showed that the adapted ICBT program is effective in reducing anxiety and depression symptoms among PDEGs. Large within-group pre-to post-treatment Cohen’s effect sizes of d = 1.23, 95% CI [0.68, 1.77] and d = 1.24, 95% CI [0.69, 1.79] were found for depression and anxiety, respectively. Further, 81.8% of the PDEGs who received the adapted ICBT reported overall satisfaction, 90.9% reported increased confidence in managing symptoms, and 70.7% completed majority of the psychoeducational lessons in the ICBT program.

Conclusion

No statistically significant differences in the clinical outcomes, engagement, and satisfaction were found between the pilot study and benchmark sample. Future directions for ICBT research with PDEGs are described.

Clinical trial registration

https://beta.clinicaltrials.gov/study/NCT05523492, identifier NCT05523492.
Mediation of the association between screen time and suicidality by overweight/obesity and perceived overweight: results from the youth risk behavior surveillance system of the United States
Authors: Gao Hong , Wang Yifei , Wang Xugang , Gao Ming
Published: March 2024

Aim

Adolescent suicide is a major public health concern, and modifiable risk factors associated with adolescent suicide remain poorly understood. This study aimed to assess the association between screen time and overweight/obesity and self-perceived overweigh and suicidality in adolescents.

Methods

Adolescents from the United States Youth Risk Behavior Surveillance System (YRBSS) between 2013 and 2019 were included in this cross-sectional study. The outcome was suicidality, including considered suicide, made a suicide plan, attempted suicide, and injurious suicide attempt. Multivariable logistic regression model was used to investigate the associations between screen time, overweight/obesity, self-perceived overweight, and suicidality, and expressed as odds ratio (OR) and 95% confidence interval (CI). Mediation analysis was used to explore the role of overweight/obesity and self-perceived overweight on the association between screen time and suicidality.

Results

A total of 30,731 adolescents were included, of which 6,350 (20.65%) had suicidality, including 5,361 (17.45%) with considered suicide, 4,432 (14.42%) with made a suicide plan, 2,300 (7.45%) with attempted suicide, and 677 (2.21%) with injurious suicide attempt. Adolescents with screen time ≥3h were related to higher odds of suicidality (OR=1.35, 95%CI: 1.23-1.46), overweight/obesity (OR=1.27, 95%CI: 1.19-1.38), and self-perceived overweight (OR=1.38, 95%CI: 1.30-1.48) after adjusting confounders. Adolescents with overweight/obesity (OR=1.30, 95%CI: 1.19-1.43) and self-perceived overweight (OR=1.54, 95%CI: 1.39-1.70) were associated with higher odds of suicidality. The association between screen time and suicidality was 4.67% mediated by overweight/obesity and 9.66% mediated by self-perceived overweight. Moreover, the mediating role of overweight/obesity was observed only in females, whereas there were no sex differences in the mediating effect of self-perceived overweight.

Conclusion

Both overweight/obesity and self-perceived overweight mediated the association between screen time and suicidality.
The effect of the cultural formulation interview on therapeutic working alliance: a study protocol
Authors: Brand Alma M. , Groen Simon P. N. , Destoop Nathalie , Jongsma Hannah E. , Ghane Samrad , Sabbe Bernard G. C. , van Velsen Harry , van Houten Kurt , Becan Özlem , Al Alyan Dhiya , Braakman Mario H.
Published: March 2024

Background

The Cultural Formulation Interview (CFI) is designed to improve understanding of patients’ mental health care needs. The lack of empirical evidence on the impact and effectiveness of CFI use in clarifying people’s perspectives, experiences, context, and identity, and in preventing cultural misunderstandings between migrant patients and clinicians, inspired this study. The objective is to examine the effect of the CFI on the strength of therapeutic working alliances, and the potential mediating or moderating role of perceived empathy.

Materials and methods

A multicenter randomized controlled trial will be conducted, involving migrant patients, their confidants, and clinicians. The CFI will be administered in the intervention group, but not in the control group. Validated questionnaires will be used to assess therapeutic working alliances and perceived empathy. T-tests and linear regression analyses will be conducted to investigate between-group differences and possible mediating or moderating effects.

Results

This study will indicate whether or not the CFI strengthens the therapeutic working alliance between patients and clinicians, as moderated and/or mediated by perceived empathy.

Discussion

Research on the effect and impact of using the CFI in mental health care for migrant patients is important to clarify whether its use strengthens the therapeutic working alliance with clinicians. This can lead to a reduction in cultural misunderstandings and improve mental health care for migrant patients. The results may also be important for the implementation of the CFI as a standard of care.

Ethics and dissemination

This research protocol was tailored to the needs of patients in collaboration with experts by experience. It was approved by the Ethical Review Board of the Tilburg Law School and registered in the Clinical Trials Register under number NCT05788315. Positive results may stimulate further implementation of the CFI in clinical practice, and contribute to improving the impact of the CFI on the therapeutic working alliances.
SARS-CoV-2 presence in recreational seawater and evaluation of intestine permeability: experimental evidence of low impact on public health
Authors: Norese Clelia , Nicosia Elena , Cortese Katia , Gentili Valentina , Rizzo Roberta , Rizzo Sabrina , Grasselli Elena , De Negri Atanasio Giulia , Gagliani Maria Cristina , Tiso Micaela , Zinni Matteo , Pulliero Alessandra , Izzotti Alberto
Published: March 2024

Introduction

Coastal seawater pollution poses a public health risk due to the potential ingestion of contaminated water during recreational activities. Wastewater-based epidemiology has revealed the abundant presence of SARS-CoV-2 in seawater emitted from wastewater outlets. The objective of this research was to investigate the impact of seawater on SARS-CoV-2 infectivity to assess the safety of recreational activities in seawater.

Methods

Wild SARS-CoV-2 was collected from oral swabs of COVID-19 affected patients and incubated for up to 90 min using the following solutions: (a) standard physiological solution (control), (b) reconstructed seawater (3.5% NaCl), and (c) authentic seawater (3.8%). Samples were then exposed to two different host systems: (a) Vero E6 cells expressing the ACE2 SARS-CoV-2 receptor and (b) 3D multi-tissue organoids reconstructing the human intestine. The presence of intracellular virus inside the host systems was determined using plaque assay, quantitative real-time PCR (qPCR), and transmission electron microscopy.

Results

Ultrastructural examination of Vero E6 cells revealed the presence of virus particles at the cell surface and in replicative compartments inside cells treated with seawater and/or reconstituted water only for samples incubated up to 2 min. After a 90-min incubation, the presence of the virus and its infectivity in Vero E6 cells was reduced by 90%. Ultrastructural analysis performed in 3D epi-intestinal tissue did not reveal intact viral particles or infection signs, despite the presence of viral nucleic acid detected by qPCR. Indeed, viral genes (Orf1ab and N) were found in the intestinal luminal epithelium but not in the enteric capillaries. These findings suggest that the intestinal tissue is not a preferential entry site for SARS-CoV-2 in the human body. Additionally, the presence of hypertonic saline solution did not increase the susceptibility of the intestinal epithelium to virus penetration; rather, it neutralized its infectivity.

Conclusion

Our results indicate that engaging in recreational activities in a seawater environment does not pose a significant risk for COVID-19 infection, despite the possible presence of viral nucleic acid deriving from degraded and fragmented viruses.