bluuebunny/crossref_metadata_2025 huggingface dataset:
A Novel Interval-Valued Decision Theoretic Rough Set Model with Intuitionistic Fuzzy Numbers Based on Power Aggregation Operators and Their Application in Medical Diagnosis
Authors: Ali Wajid , Shaheen Tanzeela , Haq Iftikhar Ul , Toor Hamza Ghazanfar , Alballa Tmader , Khalifa Hamiden Abd El-Wahed
Published: October 2023

Intuitionistic fuzzy information is a potent tool for medical diagnosis applications as it can represent imprecise and uncertain data. However, making decisions based on this information can be challenging due to its inherent ambiguity. To overcome this, power aggregation operators can effectively combine various sources of information, including expert opinions and patient data, to arrive at a more accurate diagnosis. The timely and accurate diagnosis of medical conditions is crucial for determining the appropriate treatment plans and improving patient outcomes. In this paper, we developed a novel approach for the three-way decision model by utilizing decision-theoretic rough sets and power aggregation operators. The decision-theoretic rough set approach is essential in medical diagnosis as it can manage vague and uncertain data. The redesign of the model using interval-valued classes for intuitionistic fuzzy information further improved the accuracy of the diagnoses. The intuitionistic fuzzy power weighted average (IFPWA) and intuitionistic fuzzy power weighted geometric (IFPWG) aggregation operators are used to aggregate the attribute values of the information system. The established operators are used to combine information within the intuitionistic fuzzy information system. The outcomes of various alternatives are then transformed into interval-valued classes through discretization. Bayesian decision rules, incorporating expected loss factors, are subsequently generated based on this foundation. This approach helps in effectively combining various sources of information to arrive at more accurate diagnoses. The proposed approach is validated through a medical case study where the participants are classified into three different regions based on their symptoms. In conclusion, the decision-theoretic rough set approach, along with power aggregation operators, can effectively manage vague and uncertain information in medical diagnosis applications. The proposed approach can lead to timely and accurate diagnoses, thereby improving patient outcomes.
q-Analogs of Hn,mrσ and Their Applications
Authors: Guan Hao , Koparal Sibel , Ömür Neşe , Khan Waseem Ahmad
Published: October 2023

In this paper, inspired by recent works, we define q-analogs of Hn,mσ and Hn,mrσ. By implementing them, we obtain new interesting results by taking the derivative or using generating functions.
Evolution of Medical Modeling and 3D Printing in Microvascular Midface Reconstruction: Literature Review and Experience at MD Anderson Cancer Center
Authors: Shuck John W. , Largo Rene D. , Hanasono Matthew M. , Chang Edward I.
Published: October 2023

Reconstruction of the midface represents a challenge for reconstructive microsurgeons given the formidable task of restoring both aesthetics and functionality. In particular, preservation of proper globe positioning and maintaining normal vision are as important as restoring the proper projection of the midface and enabling a patient to speak and eat as normally as possible. The introduction of virtual surgical planning (VSP) and medical modeling has revolutionized bony reconstruction of the craniofacial skeleton; however, the overwhelming majority of studies have focused on mandibular reconstruction. Here, we introduce some novel advances in utilizing VSP for bony reconstruction of the midface. The present review aims (1) to provide a review of the literature on the use of VSP in midface reconstruction and (2) to provide some insights from the authors’ early experience.
Carbapenemase-Producing Bacteria Isolated from ICU Patients of a Peruvian Government Hospital during the COVID-19 Pandemic: A Descriptive Analysis
Authors: García-Cedrón David , De La Cruz Noriega Magaly , Cabanillas-Chirinos Luis , Otiniano Nélida Milly , Rojas-Villacorta Walter , Salvatierra-Espinola Waldo , Del Aguila Karen Diaz , Luján-Velásquez Manuela
Published: October 2023

Background and Objectives: In Peru, the presence of antimicrobial-resistant bacteria is a constant concern in hospitals and has likely increased in frequency during the pandemic. The objective of the study was to analyze the frequency of carbapenemase-producing bacteria resistant to two carbapenems (Imipenem and Meropenem), which were isolated from Peruvian patients in the intensive care unit of the Victor Lazarte Echegaray Hospital in Trujillo (Peru) during the COVID-19 pandemic. Materials and Methods: The biological samples of the patients hospitalized in the ICU were processed in the Microbiology Diagnostic Laboratory of the Víctor Lazarte Echegaray Hospital between May 2021 and March 2022. Antimicrobial sensitivity was determined with the automated system AutoScan-4, and for the identification of the type of carbapenemase, the RESISIT-3 O.K.N K-SET cassettes were used. Results: The results show that 76 cultures (76/129) had resistance to the two carbapenems (imipenem or meropenem), where the most frequent were Klebsiella pneuomoniae (31.6%), Pseudomonas aeruginosa (26.3%), and Acinetobacter baumannii (14.5%). Pseudomonas aeruginosa cultures showed at least three carbapenemase types (KPC, NDM, and OXA-48), while A. baumannii, Escherichia coli, and Burkholderia cepacia complex presented at least two carbapenemases (NDM and OXA-48). The carbapenemase NDM was detected in Enterobacter cloacae, Morganella morganii, and Proteus mirabilis, while KPC was present in all Klebsiella pneumoniae and Klebsiella oxytoca cultures. Conclusions: The samples from patients hospitalized in the Victor Lazarte Echegaray Hospital ICU showed a high prevalence of imipenem- and meropenem-resistant bacteria. These findings are relevant and concerning from the perspective of antibiotic-resistant bacteria monitoring, control, and disinfection. Thus, an appropriate antibiotic policy must be implemented.
Tubularization of Bone-Tendon-Bone Grafts: Effects on Mechanical Strength and Postoperative Knee Stability in Anterior Cruciate Ligament Reconstruction
Authors: Obradović Mirko , Ninković Srđan , Gvozdenović Nemanja , Tošić Milan , Milinkov Milan , Dulić Oliver
Published: October 2023

Background and Objectives: The study addresses a significant limitation in applying bone-patellar tendon-bone (BTB) grafts in anterior cruciate ligament (ACL) surgery. By exploring the tubularization of grafts, the study extends the understanding of this surgical technique. The dual approach of the study—focusing on biomechanical properties using an animal model and postoperative outcomes in humans—offers a comprehensive perspective. Materials and Methods: The experimental cohort encompassed ten pairs of fresh porcine bone-tendon-bone grafts. One graft in each pair underwent modification through sutures that transformed the flat graft into a cylindrical structure. Testing determined the force required for the modified graft to rupture mechanically, expressed as N/mm2, compared to conventionally prepared bone-tendon-bone grafts. The second phase of the research involved a prospective randomized clinical trial comprising 120 patients undergoing operative ACL reconstruction. For half the cases, grafts were tubularized using a random selection process. Clinical evaluations preoperatively and 12 months postoperatively employed the Tegner, Lysholm, and IKDC scoring scales for knee assessment. Results: Experiments showed that ligaments made using the tubularized surgical technique have statistically significantly higher values of measured force and higher maximum elongation values than ligaments made using the classical method. The clinical study concluded that there was no significant difference between the two groups of patients in the average score on the Tegner, Lysholm, and IKDC scales before and after surgery. Conclusions: The study results showed that suturing the graft does not negatively affect its biomechanical properties, and tubularization significantly increases the values of force required to cause rupture and the values of maximum elongation during rupture. Given the possibility of the one-year follow-up period being insufficient, future investigations should extend this period to acquire objective functional insights post-surgery.
Modeling and Analysis of Metal Liquid Film Flow Characteristics during Centrifugal Spray Forming
Authors: Li Peng , Wei Shizhong , Lei Xianqing , Yang Lu , Sun Bo , Tong Shuaiwu
Published: October 2023

Liquid films are an important part of liquid metal granulation in the process of centrifugal spray forming. The size of the granulated particles has an important influence on the density, grain size and microstructure uniformity of the deposited blanks. The particle size is closely related to the flow characteristics of liquid films. Therefore, enhancing our understanding of the flow characteristics of liquid films can provide guidance for forming blanks. In this study, force analysis of a liquid film on the surface of a high-speed rotating centrifugal disc used in centrifugal spray-forming technology was carried out using D’Alembert’s principle and Newton’s law of viscosity. Then, combined with the principle of mass conservation, a theoretical model of the smooth flow of the liquid metal film was established. The experimental values obtained by Leshev were compared with our values to verify the correctness and accuracy of the model. Through the model, the influencing factors of the liquid film flow were obtained, such as the centrifugal disc speed, centrifugal disc radius, inlet volume flow rate and kinematic viscosity. Taking A390 aluminum alloy as the research object, the influence of the process parameters on the thickness, velocity and trajectory of the liquid film was revealed theoretically, and the relationship between the process parameters and the trajectory length and liquid film thickness was clarified. Modeling and analysis can not only help us to understand the flow of a liquid film, but also help us to predict the relevant parameters, which is convenient for the accurate and rapid regulation of the process to obtain the desired flow parameters. Therefore, the research content of this paper is of great significance for the preparation of billets with a uniform microstructure and excellent mechanical properties.
Exhaled Breath Analysis Using Selected Ion Flow Tube Mass Spectrometry and Disease Severity in Heart Failure
Authors: Tang Wai Hong Wilson , Tranchito Lily , Albert Chonyang , Gul Zeynep G. , Cikach Frank S. , Grove David , Wu Yuping , Dweik Raed A.
Published: October 2023

Exhaled breath volatile organic compounds (VOCs) are elevated in heart failure (HF). The ability of VOCs to predict long term cardiovascular mortality and morbidity has not been independently verified. In 55 patients admitted with acute decompensated heart failure (ADHF), we measured exhaled breath acetone and pentane levels upon admission and after 48 h of diuresis. In a separate cohort of 51 cardiac patients undergoing cardiopulmonary exercise testing (CPET), we measured exhaled breath acetone and pentane levels before and at peak exercise. In the ADHF cohort, admission acetone levels correlated with lower left ventricular ejection fraction (LVEF, r = −0.297, p = 0.035). Greater weight loss with diuretic therapy correlated with a greater reduction in both acetone levels (r = −0.398, p = 0.003) and pentane levels (r = −0.309, p = 0.021). In patients with above-median weight loss (≥4.5 kg), patients demonstrated significantly greater percentage reduction in acetone (59% reduction vs. 7% increase, p < 0.001) and pentane (23% reduction vs. 2% reduction, p = 0.008). In the CPET cohort, admission acetone and pentane levels correlated with higher VE/VCO2 (r = 0.39, p = 0.005), (r = 0.035, p = 0.014). However, there were no significant correlations between baseline or peak exercise acetone and pentane levels and peak VO2. In longitudinal follow-up with a median duration of 33 months, patients with elevated exhaled acetone and pentane levels experienced higher composite adverse events of death, ventricular assist device implantation, or orthotopic heart transplantation. In patients admitted with ADHF, higher exhaled breath acetone levels are associated with lower LVEF and poorer outcomes, and greater reductions in exhaled breath acetone and pentane tracked with greater weight loss. Exhaled acetone and pentane may be novel biomarkers in heart failure worthy of future investigation.
Report of the First Case of Candida auris Identified in Jordan
Authors: Al-Ramahi Jamal Wadi , Ghanem Rola Ali , Sayyouh Omar Helmy , Al-Jammal Dima , Said Mayar M. , Nasrat Salwa , El-Shokry Mona , Gazo Mahmoud , Osman Tamer Saied
Published: October 2023

Candida auris (C. auris) is an opportunistic budding yeast that has been identified across 41 countries, including several countries in the Middle East. The increasing global concern stems from the pathogen’s acquired resistance to antifungal drugs and its ability to form biofilms, which allows it to survive on hospital surfaces and medical devices for up to 14 days, compromising infection prevention measures in hospitals. In this report, we present the first (reported) case of C. auris isolated from a urine sample from a 48-year-old female living in Jordan. The patient succumbed to illness five days following admission to hospital. The isolate was identified retrospectively through the national surveillance system in Jordan and was confirmed by real-time PCR. Antifungal susceptibility was carried out using the microbroth dilution technique and the isolate was found to be susceptible to all tested antifungal drugs. Overall, the report emphasizes the need for active surveillance for the rapid identification of high-risk patients colonized with C. auris. It also stresses the importance of understanding the inter-clade difference in the susceptibility pattern of C. auris to facilitate the development of preventive and therapeutic strategies.
Estimated Incidence of Symptomatic Lyme Borreliosis Cases in Lublin, Poland in 2021
Authors: Colby Emily , Olsen Julia , Angulo Frederick J. , Kelly Patrick , Halsby Kate , Pilz Andreas , Sot Urszula , Chmielewski Tomasz , Pancer Katarzyna , Moïsi Jennifer C. , Jodar Luis , Stark James H.
Published: October 2023

Lyme borreliosis (LB), the most common tick-borne disease in Europe, is endemic to Poland. Despite public health surveillance with mandatory reporting of LB cases by physicians and laboratories, many symptomatic LB cases are not included in surveillance in Poland. We estimated the extent of the under-ascertainment of symptomatic LB cases via surveillance in the Polish province of Lublin to better understand Poland’s LB burden. The number of incident symptomatic LB cases in Lublin in 2010 was estimated from two seroprevalence studies conducted among adults in Lublin, as well as estimates of the proportion of asymptomatic LB cases and the duration of LB antibody persistence. The estimated number of incident symptomatic LB cases was compared to the number of surveillance-reported cases in Lublin to derive an under-ascertainment multiplier. This multiplier was applied to the number of surveillance-reported cases in 2021 to estimate the number and population-based incidence of symptomatic LB cases in Lublin in 2021. We estimate that there are 5.9 symptomatic LB cases for every surveillance-reported LB case in Lublin. Adjusting for under-ascertainment, the estimated number of symptomatic LB cases in Lublin in 2021 was 6204 (population-based incidence: 467.6/100,000). After adjustment for under-ascertainment, the incidence of symptomatic LB in Lublin, Poland, is high.
Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomy
Authors: Barreiros-Mota Inês , R. Araújo João , Marques Cláudia , Sousa Laura , Morais Juliana , Castela Inês , Faria Ana , Neto Maria Teresa , Cordeiro-Ferreira Gonçalo , Virella Daniel , Pita Ana , Pereira-da-Silva Luís , Calhau Conceição
Published: October 2023

Early-life gut dysbiosis has been associated with an increased risk of inflammatory, metabolic, and immune diseases later in life. Data on gut microbiota changes in infants undergoing intestinal surgery requiring enterostomy are scarce. This prospective cohort study examined the enterostomy effluent of 29 infants who underwent intestinal surgery due to congenital malformations of the gastrointestinal tract, necrotizing enterocolitis, or spontaneous intestinal perforation. Initial effluent samples were collected immediately after surgery and final effluent samples were collected three weeks later. Gut microbiota composition was analysed using real-time PCR and 16S rRNA gene sequencing. Three weeks after surgery, an increase in total bacteria number (+21%, p = 0.026), a decrease in Staphylococcus (−21%, p = 0.002) and Candida spp. (−16%, p = 0.045), and an increase in Lactobacillus (+3%, p = 0.045) and in less abundant genera belonging to the Enterobacteriales family were found. An increase in alpha diversity (Shannon’s and Simpson’s indexes) and significant alterations in beta diversity were observed. A correlation of necrotizing enterocolitis with higher Staphylococcus abundance and higher alpha diversity was also observed. H2-blockers and/or proton pump inhibitor therapy were positively correlated with a higher total bacteria number. In conclusion, these results suggest that positive changes occur in the gut microbiota profile of infants three weeks after intestinal surgery.
Foodborne Clostridioides Species: Pathogenicity, Virulence and Biocontrol Options
Authors: Garvey Mary
Published: October 2023

Clostridioides species possess many virulence factors and alarming levels of muti-drug resistance which make them a significant risk to public health safety and a causative agent of livestock disease. Clostridioides result in serious systemic and gastrointestinal diseases such as myonecrosis, colitis, food poisoning and gastroenteritis. As foodborne pathogens, Clostridioides species are associated with significant incidences of morbidity and mortality where the application of broad-spectrum antibiotics predisposes patients to virulent Clostridioides colonisation. As part of the One Health approach, there is an urgent need to eliminate the use of antibiotics in food production to safeguard animals, humans and the environment. Alternative options are warranted to control foodborne pathogens at all stages of food production. Antimicrobial peptides and bacteriophages have demonstrated efficacy against Clostridioides species and may offer antimicrobial biocontrol options. The bacteriocin nisin, for example, has been implemented as a biopreservative for the control of Listeria, Staphylococcus and Clostridia species in food. Bacteriophage preparations have also gained recognition for the antibacterial action against highly virulent bacterial species including foodborne pathogens. Studies are warranted to mitigate the formulation and administration limitations associated with the application of such antimicrobials as biocontrol strategies. This review outlines foodborne Clostridioides species, their virulence factors, and potential biocontrol options for application in food production.
Geological and Geomechanical Characterization of Phosphate Mine Waste Rock in View of Their Potential Civil Applications: A Case Study of the Benguerir Mine Site, Morocco
Authors: Chlahbi Safa , Belem Tikou , Elghali Abdellatif , Rochdane Samia , Zerouali Essaid , Inabi Omar , Benzaazoua Mostafa
Published: October 2023

Sedimentary phosphate extraction in open-pit operations generates large volumes of waste rock (WR), which are mainly overburdens and interburdens. Traditionally, the WR is mixed and stored on the surface in waste rock piles (WRPs). This paper presents a case study of the Benguerir mine site in Morocco. It investigates the potential valorization of each WR lithology based on the geological and geomechanical properties to reduce their environmental footprint and create added value to “waste.” The WR samples (soils and rocks) were collected from drill cores and mining trenches in the Benguerir mine. The geological characterization results using petrographic descriptions indicate the presence of nine phosphate layers and, in addition to the overburdens, eight interburdens. Four types of WR are identified: carbonate, siliceous, marly clay, and phosphate. The geomechanical characterization of soil-like samples showed an average plasticity index (PI) of 50% according to the methylene blue value (MBV) of 7.1, classifying them in the A3–A4 categories as plastic and clayey marl soils. The hard rock samples have excellent mechanical properties in terms of their uniaxial compressive strength (UCS), Los Angeles abrasion value (LA), and micro-Deval value (MD). The average compressive strength is 104 MPa for the flint, 35 MPa for the phosphate flint, 32 MPa for the silexite, 26 MPa for the limestone, 11 MPa for the indurated phosphate, and 8 MPa for the marly limestone. Based on the obtained results, these WRs can be considered as an excellent alternative secondary raw material for use in civil engineering applications, ceramics, and cement industries.
ZnS/CoS@C Derived from ZIF-8/67 Rhombohedral Dodecahedron Dispersed on Graphene as High-Performance Anode for Sodium-Ion Batteries
Authors: Jia Miao , Chen Wenfeng , He Yilin , Liu Yutong , Jia Mengqiu
Published: October 2023

Metal sulfides are highly promising anode materials for sodium-ion batteries due to their high theoretical capacity and ease of designing morphology and structure. In this study, a metal–organic framework (ZIF-8/67 dodecahedron) was used as a precursor due to its large specific surface area, adjustable pore structure, morphology, composition, and multiple active sites in electrochemical reactions. The ZIF-8/67/GO was synthesized using a water bath method by introducing graphene; the dispersibility of ZIF-8/67 was improved, the conductivity increased, and the volume expansion phenomenon that occurs during the electrochemical deintercalation of sodium was prevented. Furthermore, vulcanization was carried out to obtain ZnS/CoS@C/rGO composite materials, which were tested for their electrochemical properties. The results showed that the ZnS/CoS@C/rGO composite was successfully synthesized, with dodecahedrons dispersed in large graphene layers. It maintained a capacity of 414.8 mAh g−1 after cycling at a current density of 200 mA g−1 for 70 times, exhibiting stable rate performance with a reversible capacity of 308.0 mAh g−1 at a high current of 2 A g−1. The excellent rate performance of the composite is attributed to its partial pseudocapacitive contribution. The calculation of the diffusion coefficient of Na+ indicates that the rapid sodium ion migration rate of this composite material is also one of the reasons for its excellent performance. This study highlights the broad application prospects of metal–organic framework-derived metal sulfides as anode materials for sodium-ion batteries.
Glucosamine Improves Non-Alcoholic Fatty Liver Disease Induced by High-Fat and High-Sugar Diet through Regulating Intestinal Barrier Function, Liver Inflammation, and Lipid Metabolism
Authors: Li Feng , Zhang Zhengyan , Bai Yan , Che Qishi , Cao Hua , Guo Jiao , Su Zhengquan
Published: October 2023

Non-alcoholic fatty liver disease (NAFLD) is a liver disease syndrome. The prevalence of NAFLD has continued to increase globally, and NAFLD has become a worldwide public health problem. Glucosamine (GLC) is an amino monosaccharide derivative of glucose. GLC has been proven to not only be effective in anti-inflammation applications, but also to modulate the gut microbiota effectively. Therefore, in this study, the therapeutic effect of GLC in the NAFLD context and the mechanisms underlying these effects were explored. Specifically, an NAFLD model was established by feeding mice a high-fat and high-sugar diet (HFHSD), and the HFHSD-fed NAFLD mice were treated with GLC. First, we investigated the effect of treating NAFLD mice with GLC by analyzing serum- and liver-related indicator levels. We found that GLC attenuated insulin resistance and inflammation, increased antioxidant function, and attenuated serum and liver lipid metabolism in the mice. Then, we investigated the mechanism underlying liver lipid metabolism, inflammation, and intestinal barrier function in these mice. We found that GLC can improve liver lipid metabolism and relieve insulin resistance and oxidative stress levels. In addition, GLC treatment increased intestinal barrier function, reduced LPS translocation, and reduced liver inflammation by inhibiting the activation of the LPS/TLR4/NF-κB pathway, thereby effectively ameliorating liver lesions in NAFLD mice.
Production of Mineral-Carbon Composites and Activated Carbons as a Method of Used Gear Oil, Ashes, and Low-Quality Brown Coals Management
Authors: Wiśniewska Małgorzata , Sadłowska Amanda , Herda Karolina , Urban Teresa , Nowicki Piotr
Published: October 2023

The main objective of this study was to assess the usefulness of the low-quality brown coal, ash obtained as a result of its combustion, as well as used gear oil for the production of mineral-carbon adsorbents. The adsorbents were characterized in terms of textural parameters, acidic-basic character of the surface, mineral matter contribution to the structure, as well as their suitability for drinking water purification. Adsorption tests were carried out against two synthetic dyes—methylene blue and methyl orange. In order to understand the nature of the organic pollutants adsorption, the effect of the initial dye concentration, temperature, and pH of the system as well as the phase contact time were investigated. The obtained mineral-carbon composite and activated carbons significantly differed not only in terms of the elemental composition and chemical character of the surface (from slightly acidic to strongly alkaline), but also showed a very diverse degree of specific surface development (from 21 to 656 m2/g) and the type of porous structure generated (from micro/mesoporous to typically mesoporous). Adsorption tests showed that the efficiency of organic dye removal from aqueous solutions primarily depends on the type of the adsorbent and adsorbate applied, and, to a lesser extent, on the temperature and pH of the system. In turn, kinetic studies have shown that the sorption of dyes on such materials is consistent with a pseudo-second-order kinetics model, regardless of the type of adsorbed dye.
Multiscale Porous Carbon Materials by In Situ Growth of Metal–Organic Framework in the Micro-Channel of Delignified Wood for High-Performance Water Purification
Authors: Jeon Youngho , Kim Dabum , Lee Suji , Lee Kangyun , Ko Youngsang , Kwon Goomin , Park Jisoo , Kim Ung-Jin , Hwang Sung Yeon , Kim Jeonghun , You Jungmok
Published: October 2023

Porous carbon materials are suitable as highly efficient adsorbents for the treatment of organic pollutants in wastewater. In this study, we developed multiscale porous and heteroatom (O, N)-doped activated carbon aerogels (CAs) based on mesoporous zeolitic imidazolate framework-8 (ZIF-8) nanocrystals and wood using 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) oxidation, in situ synthesis, and carbonization/activation. The surface carboxyl groups in a TEMPO-oxidized wood (TW) can provide considerably large nucleation sites for ZIF-8. Consequently, ZIF-8, with excellent porosity, was successfully loaded into the TW via in situ growth to enhance the specific surface area and enable heteroatom doping. Thereafter, the ZIF-8-loaded TW was subjected to a direct carbonization/activation process, and the obtained activated CA, denoted as ZIF-8/TW-CA, exhibited a highly interconnected porous structure containing multiscale (micro, meso, and macro) pores. Additionally, the resultant ZIF-8/TW-CA exhibited a low density, high specific surface area, and excellent organic dye adsorption capacity of 56.0 mg cm−3, 785.8 m2 g−1, and 169.4 mg g−1, respectively. Given its sustainable, scalable, and low-cost wood platform, the proposed high-performance CA is expected to enable the substantial expansion of strategies for environmental protection, energy storage, and catalysis.
Polyoxometalate-Decorated Gold Nanoparticles Inhibit β-Amyloid Aggregation and Cross the Blood–Brain Barrier in a µphysiological Model
Authors: Perxés Perich Marta , Palma-Florez Sujey , Solé Clara , Goberna-Ferrón Sara , Samitier Josep , Gómez-Romero Pedro , Mir Mònica , Lagunas Anna
Published: October 2023

Alzheimer’s disease is characterized by a combination of several neuropathological hallmarks, such as extracellular aggregates of beta amyloid (Aβ). Numerous alternatives have been studied for inhibiting Aβ aggregation but, at this time, there are no effective treatments available. Here, we developed the tri-component nanohybrid system AuNPs@POM@PEG based on gold nanoparticles (AuNPs) covered with polyoxometalates (POMs) and polyethylene glycol (PEG). In this work, AuNPs@POM@PEG demonstrated the inhibition of the formation of amyloid fibrils, showing a 75% decrease in Aβ aggregation in vitro. As it is a potential candidate for the treatment of Alzheimer’s disease, we evaluated the cytotoxicity of AuNPs@POM@PEG and its ability to cross the blood–brain barrier (BBB). We achieved a stable nanosystem that is non-cytotoxic below 2.5 nM to human neurovascular cells. The brain permeability of AuNPs@POM@PEG was analyzed in an in vitro microphysiological model of the BBB (BBB-on-a-chip), containing 3D human neurovascular cell co-cultures and microfluidics. The results show that AuNPs@POM@PEG was able to cross the brain endothelial barrier in the chip and demonstrated that POM does not affect the barrier integrity, giving the green light to further studies into this system as a nanotherapeutic.
Epigenetic Biomarkers of Metabolic Responses to Lifestyle Interventions
Authors: Ramos-Lopez Omar
Published: October 2023

Studies have examined the possible utility of epigenetic phenomena (DNA methylation changes, covalent histone modifications, and miRNA expression patterns) in predicting individual responses to different lifestyle programs. Nonetheless, most available evidence is focused on identifying epigenetic marks eventually associated with body composition and adiposity outcomes, whereas their roles in metabolic endings remain less explored. This document comprehensively reviewed the evidence regarding the use of epigenetic signatures as putative biomarkers of metabolic outcomes (glycemic, lipid, blood pressure, and inflammatory/oxidative stress features) in response to different lifestyle interventions in humans. Although more investigation is still necessary in order to translate this knowledge in clinical practice, these scientific insights are contributing to the design of advanced strategies for the precise management of cardiometabolic risk, gaining understanding on metabolic heterogeneity, allowing for the prediction of metabolic outcomes, and facilitating the design of epigenome-based nutritional strategies for a more customized approach for metabolic alterations treatment under the scope of precision nutrition.
New Insights into the Pathogenesis and Treatment of Urinary Tract Infections
Authors: Biondo Carmelo
Published: October 2023

About 150 million people around the world experience urinary tract infections (UTI) every year, with adult women 30 times more likely to develop a UTI than men [...]
Cytotoxic Activity of Wild Plant and Callus Extracts of Ageratina pichinchensis and 2,3-Dihydrobenzofuran Isolated from a Callus Culture
Authors: Sánchez-Ramos Mariana , Encarnación-García José Guillermo , Marquina-Bahena Silvia , Sánchez-Carranza Jessica Nayelli , Bernabé-Antonio Antonio , Domínguez-Villegas Valeri , Cabañas-García Emmanuel , Cruz-Sosa Francisco
Published: October 2023

Ageratina pichinchensis (Kunth) R.M. King & H. Rob. belongs to the Asteraceae family and is a plant native to Mexico to which several biological properties are attributed. In this study, the cytotoxic effect of four extracts from the wild plants and two extracts from A. pichinchensis callus culture were evaluated against carcinogenic cell lines including prostate carcinoma, cervical cancer, hepatocellular carcinoma, hepatoma human, lung cancer, and cellular keratinocytes. The extracts were obtained with ethyl acetate and methanol using both leaves and stems or the callus. Only the ethyl acetate extract of the callus culture influenced the cervical cancer cell line (HeLa) with an IC50 of 94.79 ± 2.0 µg/mL. From the ethyl acetate callus extract, 2,3-dihydrobenzofuran was isolated and purified and also evaluated against cancer cells. The cytotoxic evaluation of this compound showed a significant effect against the HeLa cell line with an IC50 of 23.86 ± 2.5 µg/mL. Our results contribute to the development of biotechnological alternatives and extraction processes to produce compounds with possible potential against certain types of human cancer.
Extraction, Isolation, Characterization, and Biological Activity of Sulfated Polysaccharides Present in Ascidian Viscera Microcosmus exasperatus
Authors: Bento Ananda de Araujo , Maciel Marianna Cardoso , Bezerra Francisco Felipe , Mourão Paulo Antônio de Souza , Pavão Mauro Sérgio Gonçalves , Stelling Mariana Paranhos
Published: October 2023

Ascidians are marine invertebrates that synthesize sulfated glycosaminoglycans (GAGs) within their viscera. Ascidian GAGs are considered analogues of mammalian GAGs and possess great potential as bioactive compounds, presenting antitumoral and anticoagulant activity. Due to its worldwide occurrence and, therefore, being a suitable organism for large-scale mariculture in many marine environments, our main objectives are to study Microcosmus exasperatus GAGs regarding composition, structure, and biological activity. We also aim to develop efficient protocols for sulfated polysaccharides extraction and purification for large-scale production and clinical applications. GAGs derived from M. exasperatus viscera were extracted by proteolytic digestion, purified by ion-exchange liquid chromatography, and characterized by agarose gel electrophoresis and enzymatic treatments. Anticoagulant activity was evaluated by APTT assays. Antitumoral activity was assessed in an in vitro model of tumor cell culture using MTT, clonogenic, and wound healing assays, respectively. Our results show that M. exasperatus presents three distinct polysaccharides; among them, two were identified: a dermatan sulfate and a fucosylated dermatan sulfate. Antitumoral activity was confirmed for the total polysaccharides (TP). While short-term incubation does not affect tumor cell viability at low concentrations, long-term TP incubation decreases LLC tumor cell growth/proliferation at different concentrations. In addition, TP decreased tumor cell migration at different concentrations. In conclusion, we state that M. exasperatus presents great potential as an alternative GAG source, producing compounds with antitumoral properties at low concentrations that do not possess anticoagulant activity and do not enhance other aspects of malignancy, such as tumor cell migration. Our perspectives are to apply these molecules in future preclinical studies for cancer treatment as antitumoral agents to be combined with current treatments to potentiate therapeutic efficacy.
Anti-Inflammatory Effect of Cinnamomum japonicum Siebold’s Leaf through the Inhibition of p38/JNK/AP-1 Signaling
Authors: Kim Ji Min , Jung In A , Kim Jae Min , Choi Moon-Hee , Yang Ji Hye
Published: October 2023

Cinnamomum japonicum Siebold (CJ) branch bark, commonly known as Japanese cinnamon, has been used for various culinary and medicinal applications for many centuries. Although the efficacy of CJ branch bark’s anti-inflammatory and antioxidant activity for the treatment of various diseases has been confirmed, the efficacy of CJ leaves (CJLs) has not been examined. We therefore investigated whether CJL3, an ethyl acetate extract of a 70% ethanol CJL extract, exerts anti-inflammatory effects on lipopolysaccharide (LPS)-activated Kupffer cells, specialized macrophages found in the liver. Liver inflammation can activate Kupffer cells, inducing the release of pro-inflammatory molecules that contribute to tissue damage. We found that CJL3 has high 2,2-diphenyl-1-picrylhydrazyl and 2,2-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) radical-scavenging activity. Among the CJL extracts, CJL3 exhibited the greatest polyphenol content, with protocatechuic acid and 4-hydroxybenzoic acid being the most abundant. In addition, we verified that CJL3, which has strong antioxidant properties, ameliorates LPS-induced pro-inflammatory responses by inhibiting p38/JNK/AP-1 signaling. CJL3 therefore has potential for treating liver disease, including hepatitis.
Investigation and Evaluation of the Transdermal Delivery of Ibuprofen in Various Characterized Nano-Drug Delivery Systems
Authors: Myburgh Jeanri , Liebenberg Wilna , Willers Clarissa , Dube Admire , Gerber Minja
Published: October 2023

The aim was to assess the suitability of three nano-based transdermal drug delivery systems containing ibuprofen: a nano-emulsion, a nano-emulgel, and a colloidal suspension with ibuprofen-loaded nanoparticles. Understanding the transdermal delivery of ibuprofen using nano-based drug delivery systems can lead to more effective pain relief and improved patient compliance. Characterization tests assessed the suitability of the developed drug delivery systems. Membrane release and skin diffusion studies, along with tape stripping, were performed to determine drug release and skin permeation of ibuprofen. In vitro cytotoxicity studies on HaCaT cells were conducted using MTT and neutral red assays to evaluate the safety of the developed drug delivery systems. Characterization studies confirmed stable drug delivery systems with ideal properties for transdermal delivery. Membrane release studies demonstrated the successful release of ibuprofen. In vitro skin diffusion experiments and tape stripping, detecting ibuprofen in the receptor phase, stratum corneum-epidermis, and epidermis-dermis, indicating successful transdermal and topical delivery. The in vitro cytotoxicity studies observed only minor cytotoxic effects on HaCaT cells, indicating the safety of the developed drug delivery systems. The investigation demonstrated promising results for the transdermal delivery of ibuprofen using the developed drug delivery systems, which contributes to valuable insights that may lead to improved pain management strategies.
Development, Characterization and Pharmacological Evaluation of Cannabidiol-Loaded Long Circulating Niosomes
Authors: Gugleva Viliana , Ahchiyska Katerina , Georgieva Dilyana , Mihaylova Rositsa , Konstantinov Spiro , Dimitrov Erik , Toncheva-Moncheva Natalia , Rangelov Stanislav , Forys Aleksander , Trzebicka Barbara , Momekova Denitsa
Published: October 2023

Cannabidiol (CBD) is a promising drug candidate with pleiotropic pharmacological activity, whose low aqueous solubility and unfavorable pharmacokinetics have presented obstacles to its full clinical implementation. The rational design of nanocarriers, including niosomes for CBD encapsulation, can provide a plausible approach to overcoming these limitations. The present study is focused on exploring the feasibility of copolymer-modified niosomes as platforms for systemic delivery of CBD. To confer steric stabilization, the niosomal membranes were grafted with newly synthesized amphiphilic linear or star-shaped 3- and 4-arm star-shaped copolymers based on polyglycidol (PG) and poly(ε-caprolactone) (PCL) blocks. The niosomes were prepared by film hydration method and were characterized by DLS, cryo-TEM, encapsulation efficacy, and in vitro release. Free and formulated cannabidiol were further investigated for cytotoxicity and pro-apoptotic and anti-inflammatory activities in vitro in three human tumor cell lines. The optimal formulation, based on Tween 60:Span60:Chol (3.5:3.5:3 molar ration) modified with 2.5 mol% star-shaped 3-arm copolymer, is characterized by a size of 235 nm, high encapsulation of CBD (94%), and controlled release properties. Niosomal cannabidiol retained the antineoplastic activity of the free agent, but noteworthy superior apoptogenic and inflammatory biomarker-modulating effects were established at equieffective exposure vs. the free drug. Specific alterations in key signaling molecules, implicated in programmed cell death, cancer cell biology, and inflammation, were recorded with the niosomal formulations.
The Role of Bacteriophages in the Gut Microbiota: Implications for Human Health
Authors: Emencheta Stephen C. , Olovo Chinasa V. , Eze Osita C. , Kalu Chisom F. , Berebon Dinebari P. , Onuigbo Ebele B. , Vila Marta M. D. C. , Balcão Victor M. , Attama Anthony A.
Published: October 2023

Bacteriophages (phages) are nano-sized viruses characterized by their inherent ability to live off bacteria. They utilize diverse mechanisms to absorb and gain entry into the bacterial cell wall via the release of viral genetic material, which uses the replication mechanisms of the host bacteria to produce and release daughter progeny virions that attack the surrounding host cells. They possess specific characteristics, including specificity for particular or closely related bacterial species. They have many applications, including as potential alternatives to antibiotics against multi-resistant bacterial pathogens and as control agents in bacteria-contaminated environments. They are ubiquitously abundant in nature and have diverse biota, including in the gut. Gut microbiota describes the community and interactions of microorganisms within the intestine. As with bacteria, parasitic bacteriophages constantly interact with the host bacterial cells within the gut system and have obvious implications for human health. However, it is imperative to understand these interactions as they open up possible applicable techniques to control gut-implicated bacterial diseases. Thus, this review aims to explore the interactions of bacteriophages with bacterial communities in the gut and their current and potential impacts on human health.
A Large-Scale Validation of an Improved Embryo-Rescue Protocol for the Obtainment of New Table-Grape Seedless Genotypes
Authors: Chiaromonte Emanuele , Bottalico Giovanna , Lanotte Pierfederico , Campanale Antonia , Montilon Vito , Morano Massimo , Saponari Antonia , Pirolo Costantino Silvio , Gerin Donato , Faretra Francesco , Pollastro Stefania , Savino Vito Nicola
Published: October 2023

The new trends in the consumption of table grapes and the growing interest in the environmental impact of this crop have pushed breeders toward the development of seedless cultivars endowed with resistance, through crossbreeding programs. To obtain seedless grapes, the use of embryo-rescue techniques is fundamental. In this research, a grape embryo-culture protocol was optimized and validated by using 39 cultivars and 41 cross-combinations carried out in the framework of a large private table grape program of the private network Italian Variety Club in the period 2017–2021 evaluating several factors, such as the improvement in embryo formation, germination and growth, and plantlet development. The embryo culture attitude of crosses between different combinations of seedless parents was assessed, and the rates of embryo development from the extracted ovules mostly ranged from 3.5 to 35.5% with 5 out of 43 genotypes outliers. Experiments conducted at different sampling times, in a range of 43–62 days after pollination (DAP), did not show significant differences between the samples analyzed, while the rate of embryos developed with the applied protocol proved its employability on multiple genotypes, although the grapevine genotype significantly influenced the technique efficiency.
Genetic Polymorphism in the Amaranthaceae Species in the Context of Stress Tolerance
Authors: Terletskaya Nina V. , Khapilina Oxana N. , Turzhanova Ainur S. , Erbay Malika , Magzumova Saule , Mamirova Aigerim
Published: October 2023

The adaptive potential and biochemical properties of the Amaranthaceae species make them promising for introduction into agriculture and markets, particularly in arid conditions. Molecular genetic polymorphism analysis is the most powerful tool for studying plant resources; therefore, the current study aimed to investigate the polymorphisms of allelic variations in the ARF and SOD gene families, as well as the genetic diversity of six Amaranthaceae species, using retrotransposon-based fingerprinting with the multi-locus EPIC-PCR profiling approach. Additionally, the iPBS PCR amplification was employed for genome profiling, revealing variations in genetic diversity among the studied Amaranthaceae samples. The observed genetic diversity in Amaranthaceae species contributes to their enhanced tolerance to adverse environmental conditions. The knowledge about the genetic diversity of genes crucial in plant development and stress resistance can be useful for the genetic improvement of cultivated Amaranthaceae species.
Simulation-Based Identification of Operating Point Range for a Novel Laser-Sintering Machine for Additive Manufacturing of Continuous Carbon-Fibre-Reinforced Polymer Parts
Authors: Baranowski Michael , Shao Zijin , Spintzyk Alexander , Kößler Florian , Fleischer Jürgen
Published: October 2023

Additive manufacturing using continuous carbon-fibre-reinforced polymer (CCFRP) presents an opportunity to create high-strength parts suitable for aerospace, engineering, and other industries. Continuous fibres reinforce the load-bearing path, enhancing the mechanical properties of these parts. However, the existing additive manufacturing processes for CCFRP parts have numerous disadvantages. Resin- and extrusion-based processes require time-consuming and costly post-processing to remove the support structures, severely restricting the design flexibility. Additionally, the production of small batches demands considerable effort. In contrast, laser sintering has emerged as a promising alternative in industry. It enables the creation of robust parts without needing support structures, offering efficiency and cost-effectiveness in producing single units or small batches. Utilising an innovative laser-sintering machine equipped with automated continuous fibre integration, this study aims to merge the benefits of laser-sintering technology with the advantages of continuous fibres. The paper provides an outline, using a finite element model in COMSOL Multiphysics, for simulating and identifying an optimised operating point range for the automated integration of continuous fibres. The results demonstrate a remarkable reduction in processing time of 233% for the fibre integration and a reduction of 56% for the width and 44% for the depth of the heat-affected zone compared to the initial setup.
Polymer Packaging through the Blending of Biowaste Oyster Shell and Low-Density Polyethylene: A Sustainable Approach for Enhanced Food Preservation
Authors: Qu Chang-Lei , Lin Shang-Ming , Potiyaraj Pranut , Meng Lei , Wu Chin-San , Yuan Li , Luo Xin , Ge Fei-Fan , Tsou Chi-Hui
Published: October 2023

This research delves into the impact of incorporating thermally treated oyster shell powder (TOS), a biowaste filler, into low-density polyethylene (LDPE) to develop a LDPE/TOS blend, aiming at enhancing food packaging materials. The LDPE/TOS blend portrays advantageous characteristics such as augmented mechanical strength, thermostability, crystallinity, water absorption, and improved hydrophobicity with TOS content up to 50%. Microstructure analysis reveals a transition from a sparse to a more interconnected structure, contributing to the amplified tensile strength. The blend demonstrates increased barrier properties against water vapor transmission, which is attributed to elongated diffusion paths induced by the TOS particles. Application of the blend material in vegetable preservation trials manifested a substantial reduction in water loss compared to pure LDPE or no packaging. This biowaste-based blend film extends the shelf-life of chicken significantly when compared to that of pure LDPE. Importantly, the LDPE/TOS blend exhibits excellent antibacterial properties against both Escherichia coli and Staphylococcus aureus.