bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigation on Morphology and Mechanical Properties of Rod Units in Lattice Structures Fabricated by Selective Laser Melting
Authors: Jing Chenchen , Zhu Yanyan , Wang Jie , Wang Feifan , Lu Jiping , Liu Changmeng
Published: July 2021

Selective laser melting (SLM) fabrication of lattice structures has attracted considerable interest due to its many immanent advantages, such as high specific strength. A wide variety of lattice structures have been designed and fabricated. However, as a vital prerequisite for design optimization, a clear relation between the process constraint of SLM and the apparent properties of the fabricated lattice structure has received much less attention. Therefore, this work systematically investigates the characterization and preformation of rod units, which are the basic components of lattice structures, so as to evaluate the SLM manufacturability of lattice structures. A series of rod units with different inclination angles and diameters were fabricated by SLM. Their morphology and mechanical properties were measured by scanning electron microscope observation and a tensile test, respectively. The inclination angle was found to have significant effects on profile error and little effect on mechanical properties. The higher the inclination angle, the larger the profile error. The characteristic diameter had no significant correlation with profile errors and mechanical properties. Based on systematic studies, a formula is proposed to evaluate the cross-sectional area of the fabricated rod units and further estimate their load capacity. This has important implications for optimizing the design of lattice structures fabricated by SLM.
Recent Advances in Deep Reinforcement Learning Applications for Solving Partially Observable Markov Decision Processes (POMDP) Problems: Part 1—Fundamentals and Applications in Games, Robotics and Natural Language Processing
Authors: Xiang Xuanchen , Foo Simon
Published: July 2021

The first part of a two-part series of papers provides a survey on recent advances in Deep Reinforcement Learning (DRL) applications for solving partially observable Markov decision processes (POMDP) problems. Reinforcement Learning (RL) is an approach to simulate the human’s natural learning process, whose key is to let the agent learn by interacting with the stochastic environment. The fact that the agent has limited access to the information of the environment enables AI to be applied efficiently in most fields that require self-learning. Although efficient algorithms are being widely used, it seems essential to have an organized investigation—we can make good comparisons and choose the best structures or algorithms when applying DRL in various applications. In this overview, we introduce Markov Decision Processes (MDP) problems and Reinforcement Learning and applications of DRL for solving POMDP problems in games, robotics, and natural language processing. A follow-up paper will cover applications in transportation, communications and networking, and industries.
Principal Bundle Structure of Matrix Manifolds
Authors: Billaud-Friess Marie , Falcó Antonio , Nouy Anthony
Published: July 2021

In this paper, we introduce a new geometric description of the manifolds of matrices of fixed rank. The starting point is a geometric description of the Grassmann manifold Gr(Rk) of linear subspaces of dimension r<k in Rk, which avoids the use of equivalence classes. The set Gr(Rk) is equipped with an atlas, which provides it with the structure of an analytic manifold modeled on R(k−r)×r. Then, we define an atlas for the set Mr(Rk×r) of full rank matrices and prove that the resulting manifold is an analytic principal bundle with base Gr(Rk) and typical fibre GLr, the general linear group of invertible matrices in Rk×k. Finally, we define an atlas for the set Mr(Rn×m) of non-full rank matrices and prove that the resulting manifold is an analytic principal bundle with base Gr(Rn)×Gr(Rm) and typical fibre GLr. The atlas of Mr(Rn×m) is indexed on the manifold itself, which allows a natural definition of a neighbourhood for a given matrix, this neighbourhood being proved to possess the structure of a Lie group. Moreover, the set Mr(Rn×m) equipped with the topology induced by the atlas is proven to be an embedded submanifold of the matrix space Rn×m equipped with the subspace topology. The proposed geometric description then results in a description of the matrix space Rn×m, seen as the union of manifolds Mr(Rn×m), as an analytic manifold equipped with a topology for which the matrix rank is a continuous map.
Cognitive Conflict in Technological Environment: Cognitive Process and Emotions through Intuitive Errors in Area, Perimeter and Volume
Authors: Shahbari Juhaina Awawdeh
Published: July 2021

This study was conducted among 28 seventh-grade students. They worked in groups in an activity with modeling features; the activity consisted of three tasks dealing with an intuitive error, namely, same A–same B. The data source was nine video recordings of three groups across the three activities. The results obtained from analyses of students’ discussions and interactions indicate that they moved through three central stages: the intuitive error stage, the revealing of the intuitive error connected with cognitive conflict and the stage of overcoming the intuitive errors. In each of the three stages in the three tasks, we identified similar emotion features among the three groups across the three tasks. In the intuitive error stage, the participants were characterized by confidence, comfort and enjoyment. In revealing the intuitive errors, we identified several indicators and signs of non-comfortable situations by revealing the errors in the three tasks, such as a high sound or sad tone of voice, physical movements such as moving closer to the computer screen and other physical indicators such as opening the mouth and putting a hand on the head or the face. After overcoming and understanding the sources of the errors, the participants showed confidence that was clear in their facial signs, joy and smiles, loud tone and eye contact between students and the teacher, or between students.
Market and Liquidity Risks Using Transaction-by-Transaction Information
Authors: González-Sánchez Mariano , Ibáñez Jiménez Eva M. , Segovia San Juan Ana I.
Published: July 2021

The usual measures of market risk are based on the axiom of positive homogeneity while neglecting an important element of market information—liquidity. To analyze the effects of this omission, in the present study, we define the behavior of prices and volume via stochastic processes subordinated to the time elapsing between two consecutive transactions in the market. Using simulated data and market data from companies of different sizes and capitalization levels, we compare the results of measuring risk using prices compared to using both prices and volumes. The results indicate that traditional measures of market risk behave inversely to the degree of liquidity of the asset, thereby underestimating the risk of liquid assets and overestimating the risk of less liquid assets.
Metabolomic Characterization of a cf. Neolyngbya Cyanobacterium from the South China Sea Reveals Wenchangamide A, a Lipopeptide with In Vitro Apoptotic Potential in Colon Cancer Cells
Authors: Ding Lijian , Bar-Shalom Rinat , Aharonovich Dikla , Kurisawa Naoaki , Patial Gaurav , Li Shuang , He Shan , Yan Xiaojun , Iwasaki Arihiro , Suenaga Kiyotake , Zhu Chengcong , Luo Haixi , Tian Fuli , Fares Fuad , Naman C. Benjamin , Luzzatto-Knaan Tal
Published: July 2021

Metabolomics can be used to study complex mixtures of natural products, or secondary metabolites, for many different purposes. One productive application of metabolomics that has emerged in recent years is the guiding direction for isolating molecules with structural novelty through analysis of untargeted LC-MS/MS data. The metabolomics-driven investigation and bioassay-guided fractionation of a biomass assemblage from the South China Sea dominated by a marine filamentous cyanobacteria, cf. Neolyngbya sp., has led to the discovery of a natural product in this study, wenchangamide A (1). Wenchangamide A was found to concentration-dependently cause fast-onset apoptosis in HCT116 human colon cancer cells in vitro (24 h IC50 = 38 μM). Untargeted metabolomics, by way of MS/MS molecular networking, was used further to generate a structural proposal for a new natural product analogue of 1, here coined wenchangamide B, which was present in the organic extract and bioactive sub-fractions of the biomass examined. The wenchangamides are of interest for anticancer drug discovery, and the characterization of these molecules will facilitate the future discovery of related natural products and development of synthetic analogues.
Impact of Contraception on Uterine Fibroids
Authors: Kwas Katarzyna , Nowakowska Aleksandra , Fornalczyk Angelika , Krzycka Magda , Nowak Anna , Wilczyński Jacek , Szubert Maria
Published: July 2021

Background and Objectives: Uterine fibroids develop in 25–40% of women of childbearing age; however, there are discrepancies resulting from population and socioeconomic differences. The pathogenesis of fibroids is not clear. The aim of the study was to assess the potential connection between the use of oral contraceptives and the occurrence of uterine fibroids in women of childbearing age. Materials and Methods: In this prospective, survey, case–control study, data were collected from Caucasian female patients (mean age = 30) using a questionnaire concerning the onset, duration and form of hormonal contraception, and medical and obstetrical history. The questionnaires were handed personally to hospitalized patients as well as distributed through Google forms on social media. Results: In a study group (n = 140) of patients using hormonal contraception, 37.8% of them were diagnosed with uterine fibroids, whereas among the patients not using hormonal contraception (n = 206), uterine fibroids were diagnosed in 59.6% of the patients. The most common hormonal contraception was two-component hormonal tablets used by 93.3% of the patients. Taking contraceptives was a uterine fibroids protective factor (OR = 0.4, p = 0.007). In the study group, 5.5% of the patients were pregnant and 60.42% were diagnosed with uterine fibroids (OR = 4.4, p < 0.000001). Conclusion: Contraception was found to be a protective factor for uterine fibroids among the women surveyed. The presented data confirm the theory about the hormonal dependence of uterine fibroids.
Modelling Nuclear Morphology and Shape Transformation: A Review
Authors: Fang Chao , Yao Jiaxing , Xia Xingyu , Lin Yuan
Published: July 2021

As one of the most important cellular compartments, the nucleus contains genetic materials and separates them from the cytoplasm with the nuclear envelope (NE), a thin membrane that is susceptible to deformations caused by intracellular forces. Interestingly, accumulating evidence has also indicated that the morphology change of NE is tightly related to nuclear mechanotransduction and the pathogenesis of diseases such as cancer and Hutchinson–Gilford Progeria Syndrome. Theoretically, with the help of well-designed experiments, significant progress has been made in understanding the physical mechanisms behind nuclear shape transformation in different cellular processes as well as its biological implications. Here, we review different continuum-level (i.e., energy minimization, boundary integral and finite element-based) approaches that have been developed to predict the morphology and shape change of the cell nucleus. Essential gradients, relative advantages and limitations of each model will be discussed in detail, with the hope of sparking a greater research interest in this important topic in the future.
Development and Proof of Concept of a Compact Metallic Reactor for MIEC Ceramic Membranes
Authors: Escolástico Sonia , Schulze-Küppers Falk , Baumann Stefan , Haas-Santo Katja , Dittmeyer Roland
Published: July 2021

The integration of mixed ionic–electronic conducting separation membranes in catalytic membrane reactors can yield more environmentally safe and economically efficient processes. Concentration polarization effects are observed in these types of membranes when O2 permeating fluxes are significantly high. These undesired effects can be overcome by the development of new membrane reactors where mass transport and heat transfer are enhanced by adopting state-of-the-art microfabrication. In addition, careful control over the fluid dynamics regime by employing compact metallic reactors equipped with microchannels could allow the rapid extraction of the products, minimizing undesired secondary reactions. Moreover, a high membrane surface area to catalyst volume ratio can be achieved. In this work, a compact metallic reactor was developed for the integration of mixed ionic–electronic conducting ceramic membranes. An asymmetric all-La0.6Sr0.4Co0.2Fe0.8O3–δ membrane was sealed to the metallic reactor by the reactive air brazing technique. O2 permeation was evaluated as a proof of concept, and the influence of different parameters, such as temperature, sweep gas flow rates and oxygen partial pressure in the feed gas, were evaluated.
Quantification of Solid Solution Strengthening and Internal Stresses through Creep Testing of Ni-Containing Single Crystals at 980 °C
Authors: Glatzel Uwe , Schleifer Felix , Gadelmeier Christian , Krieg Fabian , Müller Moritz , Mosbacher Mike , Völkl Rainer
Published: July 2021

Various alloy compositions were cast as single crystals in a Bridgman vacuum induction furnace and creep tested at 980 °C: pure Ni, the equiatomic alloys CoCrNi and CrMnFeCoNi (Cantor alloy), single-phase fcc (Ni) solid solution alloys (with the composition of the matrix-phase of CMSX-3 and CMSX-4), and two-phase Ni-based superalloys CMSX-3 and CMSX-4. Due to the single-crystal state, grain size effects, grain boundary sliding, and grain boundary diffusion can be excluded. The results identify two major strengthening mechanisms: solid solution strengthening and other mechanisms summarized as precipitation hardening. Configurational entropy does not increase creep strength: The Cantor alloy, with the highest configurational entropy of all alloys tested, shows a weak and similar creep strength at 980 °C in comparison to pure Ni with zero configurational entropy. The element Re is a very effective strengthener, both in single-phase fcc (Ni) solid solution alloys as well as in two-phase superalloys. Quantitative estimations of different strengthening mechanisms: internal back stress, misfit stresses, Orowan bowing, and γ′-phase cutting (in the case of two-phase superalloys) are presented. Finite element simulations allow estimating the influence of solid solution strengthening of the matrix on the creep behavior of the two-phase superalloys.
A Metabolic Model of Intestinal Secretions: The Link between Human Microbiota and Colorectal Cancer Progression
Authors: Salahshouri Pejman , Emadi-Baygi Modjtaba , Jalili Mahdi , Khan Faiz M. , Wolkenhauer Olaf , Salehzadeh-Yazdi Ali
Published: July 2021

The human gut microbiota plays a dual key role in maintaining human health or inducing disorders, for example, obesity, type 2 diabetes, and cancers such as colorectal cancer (CRC). High-throughput data analysis, such as metagenomics and metabolomics, have shown the diverse effects of alterations in dynamic bacterial populations on the initiation and progression of colorectal cancer. However, it is well established that microbiome and human cells constantly influence each other, so it is not appropriate to study them independently. Genome-scale metabolic modeling is a well-established mathematical framework that describes the dynamic behavior of these two axes at the system level. In this study, we created community microbiome models of three conditions during colorectal cancer progression, including carcinoma, adenoma and health status, and showed how changes in the microbial population influence intestinal secretions. Conclusively, our findings showed that alterations in the gut microbiome might provoke mutations and transform adenomas into carcinomas. These alterations include the secretion of mutagenic metabolites such as H2S, NO compounds, spermidine and TMA (trimethylamine), as well as the reduction of butyrate. Furthermore, we found that the colorectal cancer microbiome can promote inflammation, cancer progression (e.g., angiogenesis) and cancer prevention (e.g., apoptosis) by increasing and decreasing certain metabolites such as histamine, glutamine and pyruvate. Thus, modulating the gut microbiome could be a promising strategy for the prevention and treatment of CRC.
Plant Responses to Abiotic Stresses and Rhizobacterial Biostimulants: Metabolomics and Epigenetics Perspectives
Authors: Lephatsi Motseoa M. , Meyer Vanessa , Piater Lizelle A. , Dubery Ian A. , Tugizimana Fidele
Published: July 2021

In response to abiotic stresses, plants mount comprehensive stress-specific responses which mediate signal transduction cascades, transcription of relevant responsive genes and the accumulation of numerous different stress-specific transcripts and metabolites, as well as coordinated stress-specific biochemical and physiological readjustments. These natural mechanisms employed by plants are however not always sufficient to ensure plant survival under abiotic stress conditions. Biostimulants such as plant growth-promoting rhizobacteria (PGPR) formulation are emerging as novel strategies for improving crop quality, yield and resilience against adverse environmental conditions. However, to successfully formulate these microbial-based biostimulants and design efficient application programs, the understanding of molecular and physiological mechanisms that govern biostimulant-plant interactions is imperatively required. Systems biology approaches, such as metabolomics, can unravel insights on the complex network of plant-PGPR interactions allowing for the identification of molecular targets responsible for improved growth and crop quality. Thus, this review highlights the current models on plant defence responses to abiotic stresses, from perception to the activation of cellular and molecular events. It further highlights the current knowledge on the application of microbial biostimulants and the use of epigenetics and metabolomics approaches to elucidate mechanisms of action of microbial biostimulants.
Lipoprotein Proteomics and Aortic Valve Transcriptomics Identify Biological Pathways Linking Lipoprotein(a) Levels to Aortic Stenosis
Authors: Bourgeois Raphaëlle , Bourgault Jérôme , Despres Audrey-Anne , Perrot Nicolas , Guertin Jakie , Girard Arnaud , Mitchell Patricia L. , Gotti Clarisse , Bourassa Sylvie , Scipione Corey A. , Gaudreault Nathalie , Boffa Michael B. , Koschinsky Marlys L. , Pibarot Philippe , Droit Arnaud , Thériault Sébastien , Mathieu Patrick , Bossé Yohan , Arsenault Benoit J.
Published: July 2021

Lipoprotein(a) (Lp(a)) is one of the most important risk factors for the development of calcific aortic valve stenosis (CAVS). However, the mechanisms through which Lp(a) causes CAVS are currently unknown. Our objectives were to characterize the Lp(a) proteome and to identify proteins that may be differentially associated with Lp(a) in patients with versus without CAVS. Our second objective was to identify genes that may be differentially regulated by exposure to high versus low Lp(a) levels in explanted aortic valves from patients with CAVS. We isolated Lp(a) from the blood of 21 patients with CAVS and 22 volunteers and performed untargeted label-free analysis of the Lp(a) proteome. We also investigated the transcriptomic signature of calcified aortic valves from patients who underwent aortic valve replacement with high versus low Lp(a) levels (n = 118). Proteins involved in the protein activation cascade, platelet degranulation, leukocyte migration, and response to wounding may be associated with Lp(a) depending on CAVS status. The transcriptomic analysis identified genes involved in cardiac aging, chondrocyte development, and inflammation as potentially influenced by Lp(a). Our multi-omic analyses identified biological pathways through which Lp(a) may cause CAVS, as well as key molecular events that could be triggered by Lp(a) in CAVS development.
Chronic Kidney Disease Cohort Studies: A Guide to Metabolome Analyses
Authors: Schultheiss Ulla T. , Kosch Robin , Kotsis Fruzsina , Altenbuchinger Michael , Zacharias Helena U.
Published: July 2021

Kidney diseases still pose one of the biggest challenges for global health, and their heterogeneity and often high comorbidity load seriously hinders the unraveling of their underlying pathomechanisms and the delivery of optimal patient care. Metabolomics, the quantitative study of small organic compounds, called metabolites, in a biological specimen, is gaining more and more importance in nephrology research. Conducting a metabolomics study in human kidney disease cohorts, however, requires thorough knowledge about the key workflow steps: study planning, sample collection, metabolomics data acquisition and preprocessing, statistical/bioinformatics data analysis, and results interpretation within a biomedical context. This review provides a guide for future metabolomics studies in human kidney disease cohorts. We will offer an overview of important a priori considerations for metabolomics cohort studies, available analytical as well as statistical/bioinformatics data analysis techniques, and subsequent interpretation of metabolic findings. We will further point out potential research questions for metabolomics studies in the context of kidney diseases and summarize the main results and data availability of important studies already conducted in this field.
Epidemiology of African Swine Fever and Its Risk in Nepal
Authors: Subedi Deepak , Bhandari Suman , Pantha Saurav , Poudel Uddab , Jyoti Sumit , Kandel Milan , Karki Surendra , Dhakal Santosh
Published: July 2021

African swine fever (ASF) is a highly contagious viral infection of domestic and wild pigs with high mortality. First reported in East Africa in the early 1900s, ASF was largely controlled in domestic pigs in many countries. However, in recent years ASF outbreaks have been reported in several countries in Europe and Asia. The occurrence of ASF in China, the largest pork producer in the world, in 2018 and in India, the country that surrounds and shares open borders with Nepal, has increased the risk of ASF transmission to Nepal. Lately, the pork industry has been growing in Nepal, overcoming traditional religious and cultural biases against it. However, the emergence of viral infections such as ASF could severely affect the industry’s growth and sustainability. Because there are no effective vaccines available to prevent ASF, the government should focus on preventing entry of the virus through strict quarantine measures at the borders, controls on illegal trade, and effective management practices, including biosecurity measures.
Viruses and Type 1 Diabetes: From Enteroviruses to the Virome
Authors: Isaacs Sonia R. , Foskett Dylan B. , Maxwell Anna J. , Ward Emily J. , Faulkner Clare L. , Luo Jessica Y. X. , Rawlinson William D. , Craig Maria E. , Kim Ki Wook
Published: July 2021

For over a century, viruses have left a long trail of evidence implicating them as frequent suspects in the development of type 1 diabetes. Through vigorous interrogation of viral infections in individuals with islet autoimmunity and type 1 diabetes using serological and molecular virus detection methods, as well as mechanistic studies of virus-infected human pancreatic β-cells, the prime suspects have been narrowed down to predominantly human enteroviruses. Here, we provide a comprehensive overview of evidence supporting the hypothesised role of enteroviruses in the development of islet autoimmunity and type 1 diabetes. We also discuss concerns over the historical focus and investigation bias toward enteroviruses and summarise current unbiased efforts aimed at characterising the complete population of viruses (the “virome”) contributing early in life to the development of islet autoimmunity and type 1 diabetes. Finally, we review the range of vaccine and antiviral drug candidates currently being evaluated in clinical trials for the prevention and potential treatment of type 1 diabetes.
Alterations and Contaminations in Ceramics Deposited in Underwater Environments: An Experimental Approach
Authors: Sanchez-Garmendia Uxue , Iñañez Javier G. , Arana Gorka
Published: July 2021

Ancient ceramics recovered after a long burial period have probably undergone several alterations and contaminations, introducing a chemical variability, affecting the ceramic’s natural variability. That is, the chemical and the mineralogical compositions of the ceramic pastes after their deposition will not be the same as they originally were. Therefore, it is known that the alteration and contamination processes, and the discrimination of some elements, should be considered when studying the ceramics to avoid incorrect interpretations about their provenance, technology and the use of the artefact, as well as its proper preservation. In the present work, the authors performed an experimental approach in order to study the alterations and contaminations that occurred in 60 ceramic cylinders buried in two different underwater environments. Once the pieces were taken out from the water environments, they were characterized by a multi-analytical approach. For this purpose, inductively coupled plasma mass spectrometry (ICP-MS), X-ray diffraction (XRD), scanning electron microscopy–energy dispersive spectrometry (SEM–EDS) and Raman spectroscopy were used. Newly formed minerals of different forms have been identified, with different crystallization grades. Some examples are the needles, flakes, sponges and long and short prisms composed of several elements such as Ca, F, S and O.
Structure and IR Spectra of 3(5)-Aminopyrazoles and UV-Induced Tautomerization in Argon Matrix
Authors: Secrieru Alina , Lopes Susy , Cristiano Maria L. S. , Fausto Rui
Published: July 2021

The prototropic tautomerism in 3(5)-aminopyrazoles was investigated by matrix isolation infrared (IR) spectroscopy, supported by DFT(B3LYP)/6-311++G(d,p) calculations. In consonance with the experimental data, the calculations predict tautomer 3-aminopyrazole (3AP) to be more stable than the 5-aminopyrazole (5AP) tautomer (calculated energy difference: 10.7 kJ mol−1; Gibbs free energy difference: 9.8 kJ mol−1). The obtained matrix isolation IR spectra (in both argon and xenon matrices) were interpreted, and the observed bands were assigned to the tautomeric forms with help of vibrational calculations carried out at both harmonic and anharmonic levels. The matrix-isolated compound (in argon matrix) was then subjected to in situ broadband UV irradiation (λ > 235 nm), and the UV-induced transformations were followed by IR spectroscopy. Phototautomerization of the 3AP tautomer into the 5AP form was observed as the strongly prevalent reaction.
Ferroptosis-Inhibitory Difference between Chebulagic Acid and Chebulinic Acid Indicates Beneficial Role of HHDP
Authors: Yang Lin , Liu Yangping , Zhang Wenhui , Hua Yujie , Chen Ban , Wu Quanzhou , Chen Dongfeng , Liu Shuqin , Li Xican
Published: July 2021

The search for a safe and effective inhibitor of ferroptosis, a recently described cell death pathway, has attracted increasing interest from scientists. Two hydrolyzable tannins, chebulagic acid and chebulinic acid, were selected for the study. Their optimized conformations were calculated using computational chemistry at the B3LYP-D3(BJ)/6-31G and B3LYP-D3(BJ)/6-311 + G(d,p) levels. The results suggested that (1) chebulagic acid presented a chair conformation, while chebulinic acid presented a skew-boat conformation; (2) the formation of chebulagic acid requires 762.1729 kcal/mol more molecular energy than chebulinic acid; and (3) the 3,6-HHDP (hexahydroxydiphenoyl) moiety was shown to be in an (R)- absolute stereoconfiguration. Subsequently, the ferroptosis inhibition of both tannins was determined using a erastin-treated bone marrow-derived mesenchymal stem cells (bmMSCs) model and compared to that of ferrostatin-1 (Fer-1). The relative inhibitory levels decreased in the following order: Fer-1 > chebulagic acid > chebulinic acid, as also revealed by the in vitro antioxidant assays. The UHPLC–ESI-Q-TOF-MS analysis suggested that, when treated with 16-(2-(14-carboxytetradecyl)-2-ethyl-4,4-dimethyl-3-oxazolidinyloxy free radicals, Fer-1 generated dimeric products, whereas the two acids did not. In conclusion, two hydrolyzable tannins, chebulagic acid and chebulinic acid, can act as natural ferroptosis inhibitors. Their ferroptosis inhibition is mediated by regular antioxidant pathways (ROS scavenging and iron chelation), rather than the redox-based catalytic recycling pathway exhibited by Fer-1. Through antioxidant pathways, the HHDP moiety in chebulagic acid enables ferroptosis-inhibitory action of hydrolyzable tannins.
Characterization of Citrusnobilis Peel Methanolic Extract for Antioxidant, Antimicrobial, and Anti-Inflammatory Activity
Authors: Malik Anjali , Najda Agnieszka , Bains Aarti , Nurzyńska-Wierdak Renata , Chawla Prince
Published: July 2021

Currently, the potential utilization of fruits and vegetable waste as a source of micronutrients and antioxidants has increased. The present study, therefore, aimed to determine the antimicrobial and anti-inflammatory activities of Citrus nobilis peel extract. A modified solvent evaporation technique was employed for peel extract preparation. For effective utilization of the natural product, quantitative analysis of phenolic compounds was carried out using liquid chromatography and mass spectroscopy technique. Phenolic and flavonoids were present in high amounts, while β-carotene and lycopene were present in vestigial amounts. The antimicrobial efficiency of peel extract was evaluated against four bacterial strains including Staphylococcus aureus (MTCC 3160), Klebsiella pneumoniae (MTCC 3384), Pseudomonas aeruginosa (MTCC 2295), and Salmonella typhimurium (MTCC 1254), and one fungal strain Candida albicans (MTCC 183), and zone of inhibition was comparable to the positive control streptomycin and amphotericin B, respectively. The extract of Citrus nobilis peels showed effective anti-inflammatory activity during human red blood cell membrane stabilization (HRBC) and albumin denaturation assay. The extracts also exhibited 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity ranging from 53.46 to 81.13%. Therefore, the obtained results suggest that Citrus nobilis peel could be used as an excellent source of polyphenols and transformed into value-added products.
Adsorption Properties of Hydrocarbon and Fluorocarbon Surfactants Ternary Mixture at the Water-Air Interface
Authors: Jańczuk Bronisław , Szymczyk Katarzyna , Zdziennicka Anna
Published: July 2021

Measurements were made of the surface tension of the aqueous solutions of p-(1,1,3,3-tetramethylbutyl) phenoxypoly(ethylene glycols) having 10 oxyethylene groups in the molecule (Triton X-100, TX100) and cetyltrimethylammonium bromide (CTAB) with Zonyl FSN-100 (FC6EO14, FC1) as well as with Zonyl FSO-100 (FC5EO10, FC2) ternary mixtures. The obtained results were compared to those provided by the Fainerman and Miller equation and to the values of the solution surface tension calculated, based on the contribution of a particular surfactant in the mixture to the reduction of water surface tension. The changes of the aqueous solution ternary surfactants mixture surface tension at the constant concentration of TX100 and CTAB mixture at which the water surface tension was reduced to 60 and 50 mN/m as a function of fluorocarbon surfactant concentration, were considered with regard to the composition of the mixed monolayer at the water-air interface. Next, this composition was applied for the calculation of the concentration of the particular surfactants in the monolayer using the Frumkin equation. On the other hand, the Gibbs surface excess concentration was determined only for the fluorocarbon surfactants. The tendency of the particular surfactants to adsorb at the water-air interface was discussed, based on the Gibbs standard free energy of adsorption which was determined using different methods. This energy was also deduced, based on the surfactant tail surface tension and tail-water interface tension.
Nanotechnology Integration for SARS-CoV-2 Diagnosis and Treatment: An Approach to Preventing Pandemic
Authors: Asdaq Syed Mohammed Basheeruddin , Ikbal Abu Md Ashif , Sahu Ram Kumar , Bhattacharjee Bedanta , Paul Tirna , Deka Bhargab , Fattepur Santosh , Widyowati Retno , Vijaya Joshi , Al mohaini Mohammed , Alsalman Abdulkhaliq J. , Imran Mohd. , Nagaraja Sreeharsha , Nair Anroop B. , Attimarad Mahesh , Venugopala Katharigatta N.
Published: July 2021

The SARS-CoV-2 outbreak is the COVID-19 disease, which has caused massive health devastation, prompting the World Health Organization to declare a worldwide health emergency. The corona virus infected millions of people worldwide, and many died as a result of a lack of particular medications. The current emergency necessitates extensive therapy in order to stop the spread of the coronavirus. There are various vaccinations available, but no validated COVID-19 treatments. Since its outbreak, many therapeutics have been tested, including the use of repurposed medications, nucleoside inhibitors, protease inhibitors, broad spectrum antivirals, convalescence plasma therapies, immune-modulators, and monoclonal antibodies. However, these approaches have not yielded any outcomes and are mostly used to alleviate symptoms associated with potentially fatal adverse drug reactions. Nanoparticles, on the other hand, may prove to be an effective treatment for COVID-19. They can be designed to boost the efficacy of currently available antiviral medications or to trigger a rapid immune response against COVID-19. In the last decade, there has been significant progress in nanotechnology. This review focuses on the virus’s basic structure, pathogenesis, and current treatment options for COVID-19. This study addresses nanotechnology and its applications in diagnosis, prevention, treatment, and targeted vaccine delivery, laying the groundwork for a successful pandemic fight.
Construction of Novel Nanocomposites (Cu-MOF/GOD@HA) for Chemodynamic Therapy
Authors: Hao Ya-Nan , Qu Cong-Cong , Shu Yang , Wang Jian-Hua , Chen Wei
Published: July 2021

The emerging chemodynamic therapy (CDT) has received an extensive attention in recent years. However, the efficiency of CDT is influenced due to the limitation of H2O2 in tumor. In this study, we designed and synthesized a novel core-shell nanostructure, Cu-metal organic framework (Cu-MOF)/glucose oxidase (GOD)@hyaluronic acid (HA) (Cu-MOF/GOD@HA) for the purpose of improving CDT efficacy by increasing H2O2 concentration and cancer cell targeting. In this design, Cu-MOF act as a CDT agent and GOD carrier. Cu(II) in Cu-MOF are reduced to Cu(I) by GSH to obtain Cu(I)-MOF while GSH is depleted. The depletion of GSH reinforces the concentration of H2O2 in tumor to improve the efficiency of CDT. The resultant Cu(I)-MOF catalyze H2O2 to generate hydroxyl radicals (·OH) for CDT. GOD can catalyze glucose (Glu) to supply H2O2 for CDT enhancement. HA act as a targeting molecule to improve the targeting ability of Cu-MOF/GOD@HA to the tumor cells. In addition, after loading with GOD and coating with HA, the proportion of Cu(I) in Cu-MOF/GOD@HA is increased compared with the proportion of Cu(I) in Cu-MOF. This phenomenon may shorten the reactive time from Cu-MOF to Cu(I)-MOF. The CDT enhancement as a result of GOD and HA effects in Cu-MOF/GOD@HA was evidenced by in vitro cell and in vivo animal studies.
Food Insecurity and Food Label Comprehension among Libyan Migrants in Australia
Authors: Mansour Reima , John James Rufus , Liamputtong Pranee , Arora Amit
Published: July 2021

Food security among migrants and refugees remains an international public health issue. However, research among ethnic minorities in Australia is relatively low. This study explored the factors that influence the understanding of food labelling and food insecurity among Libyan migrants in Australia. An online survey was completed by 271 Libyan migrant families. Data collection included the 18-item US Household Food Security Survey Module (for food security) and a question from the Food Standards Australia New Zealand Consumer Label Survey (for food labelling comprehension). Multivariable logistic regression modelling was utilised to identify the predictors of food label comprehension and food security. Food insecurity prevalence was 72.7% (n = 196) while 35.8% of families (n = 97) reported limited food label understanding. Household size, food store location, and food affordability were found to be significantly related to food insecurity. However, gender, private health insurance, household annual income, education, and food store type and location were found to be significantly related to food labelling comprehension. Despite the population’s high educational status and food labelling comprehension level, food insecurity remained an issue among the Libyan migrants. Policy makers should consider the incorporation of food label comprehension within a broader food security approach for migrants.
Effect of Extra Virgin Olive Oil and Butter on Endothelial Function in Type 1 Diabetes
Authors: Cutruzzolà Antonio , Parise Martina , Vallelunga Rosarina , Lamanna Francesco , Gnasso Agostino , Irace Concetta
Published: July 2021

Post-prandial hyperglycemia can be relevant in developing early manifestations of atherosclerosis. EVOO (Extra Virgin Olive Oil), rich in saturated fatty acids and commonly used in the Mediterranean diet, seems to control post-prandial hyperglycemia better than butter. Subjects with type 1 diabetes are at higher risk of developing cardiovascular disease and show endothelial dysfunction, an early manifestation of atherosclerosis in the first years of the disease. Our study aims to evaluate whether EVOO and butter influence endothelial function in subjects with type 1 diabetes when added to a single high glycemic index (HGI) meal. In this exploratory cross-over study, 10 subjects with type 1 diabetes and 6 healthy subjects were scheduled to receive two types of HGI meals: one enriched with EVOO and one with butter. Before and after each test meal at different time points, all subjects underwent the evaluation of endothelial function by flow-mediated dilation technique, glucose and lipids measurements, and gastric emptying assessment by ultrasound. Flow-mediated dilation significantly increased after EVOO-enriched meal compared with butter in subjects with type 1 diabetes (two-way-repeated measurements ANOVA, p = 0.007). In patients with type 1 diabetes, the add-on of EVOO to HGI meal improves vascular function compared to butter, which has detrimental effects.
Consumption of Monounsaturated Fatty Acids Is Associated with Improved Cardiometabolic Outcomes in Four African-Origin Populations Spanning the Epidemiologic Transition
Authors: Mehta Supal , Ruth Dugas Lara , Choo-Kang Candice , Bovet Pascal , Forrester Terrence , Bedu-Addo Kweku , Lambert Estelle Vicki , Plange-Rhule Jacob , Riesen Walter , Korte Wolfgang , Luke Amy
Published: July 2021

Long-chain omega-3 PUFAs, specifically eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are of increasing interest because of their favorable effect on cardiometabolic risk. This study explores the association between omega 6 and 3 fatty acids intake and cardiometabolic risk in four African-origin populations spanning the epidemiological transition. Data are obtained from a cohort of 2500 adults aged 25–45 enrolled in the Modeling the Epidemiologic Transition Study (METS), from the US, Ghana, Jamaica, and the Seychelles. Dietary intake was measured using two 24 h recalls from the Nutrient Data System for Research (NDSR). The prevalence of cardiometabolic risk was analyzed by comparing the lowest and highest quartile of omega-3 (EPA+ DHA) consumption and by comparing participants who consumed a ratio of arachidonic acid (AA)/EPA + DHA ≤4:1 and >4:1. Data were analyzed using multiple variable logistic regression adjusted for age, gender, activity, calorie intake, alcohol intake, and smoking status. The lowest quartile of EPA + DHA intake is associated with cardiometabolic risk 2.16 (1.45, 3.2), inflammation 1.59 (1.17, 2.16), and obesity 2.06 (1.50, 2.82). Additionally, consuming an AA/EPA + DHA ratio of >4:1 is also associated with cardiometabolic risk 1.80 (1.24, 2.60), inflammation 1.47 (1.06, 2.03), and obesity 1.72 (1.25, 2.39). Our findings corroborate previous research supporting a beneficial role for monounsaturated fatty acids in reducing cardiometabolic risk.
Efficacy of an Anti-Cellulite Herbal Emgel: A Randomized Clinical Trial
Authors: Ngamdokmai Ngamrayu , Waranuch Neti , Chootip Krongkarn , Jampachaisri Katechan , Scholfield C. Norman , Ingkaninan Kornkanok
Published: July 2021

Cellulite describes unsightly skin overlying subcutaneous fat around thighs and buttocks of post-pubescent females. A herbal ‘emgel’ containing volatile oils and extracts of A traditional Thai herbal compress was tested in a double-blind, placebo-controlled trial with 18 women aged 20–50 year with severe cellulite. Appearance of cellulite (primary outcome), thigh circumferences, skin firmness, and cutaneous blood flow (secondary outcomes) were assessed at baseline, 2, 4, 8 and 12 weeks with a 2-week follow-up. Herbal emgel applied onto the thigh skin twice daily reduced cellulite severity scores in every time point. The score was reduced from 13.4 ± 0.3 (baseline) to 12.1 ± 0.3 (week 2) and 9.9 ± 0.6 (week 12). All secondary outcomes improved with both placebo and herbal emgels suggesting that ingredients in the base-formulation might be responsible. Querying of participants, analysis of their diaries, and physical monthly inspections found no adverse events. The herbal emgel safely improved the appearance of cellulite, while the base emgel may play a role for other endpoints. Further studies on the active constituents and their mechanism of action are needed to further explore these factors.
Delaying Effects of Prolactin and Growth Hormone on Aging Processes in Bovine Oocytes Matured In Vitro
Authors: Singina Galina N. , Shedova Ekaterina N. , Lopukhov Alexander V. , Mityashova Olga S. , Lebedeva Irina Y.
Published: July 2021

Aging processes accelerate dramatically in oocytes that have reached the metaphase-II (M-II) stage. The present work aimed to study the patterns and intracellular pathways of actions of prolactin (PRL) and growth hormone (GH) on age-associated changes in bovine M-II oocytes aging in vitro. To this end, we analyzed spontaneous parthenogenetic activation (cytogenetic assay), apoptosis (TUNEL assay), and the developmental capacity (IVF/IVC) of in vitro-matured oocytes after prolonged culturing. Both PRL and GH reduced the activation rate of aging cumulus-enclosed oocytes (CEOs) and denuded oocytes (DOs), and their respective hormone receptors were revealed in the ova. The inhibitor of Src-family tyrosine kinases PP2 eliminated the effects of PRL and GH on meiotic arrest in DOs, whereas the MEK inhibitor U0126 only abolished the PRL effect. Furthermore, PRL was able to maintain the apoptosis resistance and developmental competence of aging CEOs. The protein kinase C inhibitor calphostin C suppressed both the actions of PRL. Thus, PRL and GH can directly support meiotic arrest in aging M-II oocytes by activating MAP kinases and/or Src-family kinases. The effect of PRL in maintaining the developmental capacity of aging oocytes is cumulus-dependent and related to the pro-survival action of the protein kinase C-mediated signal pathway.
Olive Oil Lipophenols Induce Insulin Secretion in 832/13 β-Cell Models
Authors: Caroleo Maria Cristina , Plastina Pierluigi , Fazio Alessia , La Torre Chiara , Manetti Fabrizio , Cione Erika
Published: July 2021

Glycemic control is a mainstay of type 2 diabetes mellitus (T2DM) clinical management. Despite the continuous improvement in knowledge and progress in terms of treatment, the achievement of the physiologic metabolic profile is still an ongoing challenge in diabetic patients. Pancreatic β-cell line INS-1 832/13 was used to assess the insulin secretagogue activity of hydroxytyrosyl oleate (HtyOle) and tyrosyl oleate (TyOle), two naturally occurring lipophenols deriving from the conjugation of oleic acid (OA) and hydroxytyrosol (Hty) or tyrosol (Ty), respectively. The insulin secretion was determined under a glucose-induced insulin secretion (GSIS) condition by the ELISA method. The potential involvement of G-protein-coupled receptor 40 (GPR40), also known as free fatty acid receptor 1 (FFAR1), was investigated by both molecular docking and functional pharmacological approaches. Herein, we demonstrated that HtyOle and TyOle exerted a facilitatory activity on insulin secretion under the GSIS condition. Moreover, we provided evidence that both lipophenols are natural modulators of FFAR1 receptor. From our results, the anti-diabetes properties associated with olive oil consumption can be partly explained by the HtyOle and TyOle effects.
Medicated Hydroxyapatite/Collagen Hybrid Scaffolds for Bone Regeneration and Local Antimicrobial Therapy to Prevent Bone Infections
Authors: Mulazzi Manuela , Campodoni Elisabetta , Bassi Giada , Montesi Monica , Panseri Silvia , Bonvicini Francesca , Gentilomi Giovanna Angela , Tampieri Anna , Sandri Monica
Published: July 2021

Microbial infections occurring during bone surgical treatment, the cause of osteomyelitis and implant failures, are still an open challenge in orthopedics. Conventional therapies are often ineffective and associated with serious side effects due to the amount of drugs administered by systemic routes. In this study, a medicated osteoinductive and bioresorbable bone graft was designed and investigated for its ability to control antibiotic drug release in situ. This represents an ideal solution for the eradication or prevention of infection, while simultaneously repairing bone defects. Vancomycin hydrochloride and gentamicin sulfate, here considered for testing, were loaded into a previously developed and largely investigated hybrid bone-mimetic scaffold made of collagen fibers biomineralized with magnesium doped-hydroxyapatite (MgHA/Coll), which in the last ten years has widely demonstrated its effective potential in bone tissue regeneration. Here, we have explored whether it can be used as a controlled local delivery system for antibiotic drugs. An easy loading method was selected in order to be reproducible, quickly, in the operating room. The maintenance of the antibacterial efficiency of the released drugs and the biosafety of medicated scaffolds were assessed with microbiological and in vitro tests, which demonstrated that the MgHA/Coll scaffolds were safe and effective as a local delivery system for an extended duration therapy—promising results for the prevention of bone defect-related infections in orthopedic surgeries.