bluuebunny/crossref_metadata_2025 huggingface dataset:
Book Launch and Scientific Workshop "Follow Your Heart. The School for Multipotentialites"
Authors: Poto Margherita Paola , Murray Emily Margaret , Russo Valentina
Published: March 2023

The overall aim of this report is to document the experience gained during the book launch and co-created, experiential workshop on the book "Follow Your Heart: the school for multipotentialites." Reflecting on the dynamic process triggered by the research gathered and further developed throughout the book helps to create ideas and practices toward transformative change in research and education. The authors planned three book launches, structured as innovative academic presentations combined with creativity workshops that follow some of the suggested activities that were specifically created for the book. The first book launch was held in Shepton Mallet, Somerset, UK, and hosted by the educational farm The Good Life Projects, coordinated by Craig Stevens, on February 18, 2023. The proceeds from the book launch (collected through book sales) were donated to Windsor Hill Wood, a place of refuge and community in the local area that helps people heal, grow, and rebuild their lives while taking care of nature and encouraging sustainable livelihoods. Windsor Hill Wood as a community and philosophy closely align with the authors' beliefs and the main concepts of Follow Your Heart, which made the overall impact of the first book launch exceptionally more meaningful. This report is designed to share the key insights from the event and promote further discussion within the field of emotional and ecological education, in line with the project ECO_CARE. The report is accordingly divided into the following sections: 1. Introduction; 2. Follow Your Heart: audience, vision and development; 3. Ways forward; 4. Reference list.
Genome-wide survey of HMA gene family and its characterization in wheat (Triticum aestivum)
Authors: Zahra Sadaf , Shaheen Tayyaba , Qasim Muhammad , Mahmood-ur-Rahman  , Hussain Momina , Zulfiqar Sana , Shaukat Kanval , Mehboob-ur-Rahman
Published: March 2023

Background

Abiotic stresses, particularly drought and heavy metal toxicity, have presented a significant risk to long-term agricultural output around the world. Although the heavy-metal-associated domain (HMA) gene family has been widely explored in Arabidopsis and other plants, it has not been thoroughly studied in wheat (Triticum aestivum). This study was proposed to investigate the HMA gene family in wheat.

Methods

To analyze the phylogenetic relationships, gene structure, gene ontology, and conserved motifs, a comparative study of wheat HMA genes with the Arabidopsis genome was performed.

Results

A total of 27 T. aestivum proteins belonging to the HMA gene family were identified in this study, with amino acid counts ranging from 262 to 1,071. HMA proteins were found to be grouped into three subgroups in a phylogenetic tree, and closely related proteins in the tree showed the same expression patterns as motifs found in distinct subgroups. Gene structural study elucidated that intron and exon arrangement differed by family.

Conclusion

As a result, the current work offered important information regarding HMA family genes in the T. aestivum genome, which will be valuable in understanding their putative functions in other wheat species.
Long-term outcomes and predictors of patients with ST elevated versus non-ST elevated myocardial infarctions in non-obstructive coronary arteries: a retrospective study in Northern China
Authors: Chen Lin , Fan Yinghong , Fang Zhen , Liu Ning
Published: March 2023

Background

Myocardial infarction with non-obstructive coronary arteries (MINOCA) is a heterogeneous disease entity with diverse etiologies and no uniform treatment protocols. Patients with MINOCA can be clinically classified into two groups based on whether they have an ST-segment elevation (STE) or non-ST segment elevation (NSTE), based on electrocardiogram (ECG) results, whose clinical prognosis is unclear. This study aimed to compare the outcomes and predictors of patients with STE and NSTE in the MINOCA population.

Methods

We collected the data for 196 patients with MINOCA (115 with STE and 81 with NSTE) in China. Clinical characteristics, prognoses, and predictors of major adverse cardiovascular events (MACE) were analyzed during the follow-up of all patients.

Results

The proportion of patients with STE was greater than that with NSTE in the MINOCA population. Patients with NSTE were older and had a higher incidence of hypertension. No differences were observed in the outcomes between the STE and NSTE groups during a median follow-up period of 49 (37,46) months. No significant differences were observed in those with MACE (24.35% vs 22.22%, P = 0.73) and those without MACE. The multivariable predictors of MACE in the NSTE groups were Killip grades ≥ 2 (HR 9.035, CI 95% [1.657–49.263], P = 0.011), reduced use of β-blockers during hospitalization (HR 0.238, CI 95% [0.072–0.788], P = 0.019), and higher levels of low-density lipoprotein cholesterol (LDL-C) (HR 2.267, CI 95% [1.008–5.097], P = 0.048); the reduced use of β-blockers during hospitalization was the only independent risk factor of MACE in the STE group.

Conclusions

There were differences between the clinical characteristics of patients with STE and NSTE in the MINOCA population, even though outcomes during follow-up were similar. Independent risk factors for major adverse cardiac events were not identical in the STE and NSTE groups, which could be attributable to the differences in disease pathogenesis.
Factors Affecting Quality of Life in Hemodialysis Patients
Authors: Lim Kyung Hee , Kwon Geum Soon
Published: March 2023

Purpose: The purpose of this study was to investigate factors affecting quality of life in hemodialysis patients.Methods: In this descriptive study, the subjects were 127 hemodialysis patients at four dialysis clinics in Pusan and Daegu metropolitan cities. Data were analyzed with descriptive statistics, independent t-test, one-way analysis of variance, Pearson’s correlation coefficients, and stepwise multiple regression analysis using SPSS/WIN version 26.0.Results: The average scores for social support, symptom experience, physical function, self-care compliance, and quality of life were 3.97±0.66, 38.50±27.94, 2.24±0.60, 3.43±0.49, and 3.02±0.57, respectively. Fifty-five (43.3%) subjects had a type D personality. Quality of life showed significant differences according to educational background (F=6.44, p<.001), job (t=2.52, p=.013), and type D personality (t=4.39, p<.001). Social support (r=.32, p<.001), physical function (r=.45, p<.001), self-care compliance (r=.24, p=.005), albumin (r=.09, p=.027), and phosphorous (r=.27, p=.002) were positively correlated with quality of life. Symptom experience (r=-.40, p<.001) was negatively correlated with quality of life. The factors affecting quality of life were social support (β=.31, p<.001), physical function (β=.30, p<.001), educational background (β=.20, p=.007), type D personality (β=-.19, p<.001), and symptom experience (β=-.18, p=.019), with a total explanatory power of approximately 43%.Conclusion: Quality of life in hemodialysis patients was affected by social support, physical function, educational background, type D personality, and symptom experience. These factors should be considered in nursing interventions to improve quality of life of hemodialysis patients.
The Influence of Long-term Care Hospital Nurses' Nursing Professionalism and Knowledge of COVID-19 on Nursing Intentions for COVID-19 Patients
Authors: Lee Mi Aie , Park Hyun Ju , Kim Bonghwa
Published: March 2023

Purpose: This study attempted to identify the influence of long-term care hospital nurses' nursing professionalism and knowledge of coronavirus disease 2019 (COVID-19) on nursing intentions for COVID-19 patients.Methods: Data were collected from nurses who had worked for at least 6 months at long-term care hospitals with more than 100 beds located in North Gyeongsang Province from July 15 to 31, 2021. In total, 207 questionnaires were analyzed using the t-test, one-way analysis of variance, Pearson correlation coefficients, and multiple linear regression with SPSS for Windows version 25.0.Results: Among the sub-factors constituting nursing professionalism, self-concept of the profession (r=.22, p=.001) and professionalism of nursing (r=.23, p=.001) were correlated with nursing intention for COVID-19 patients, whereas there no correlation was found between knowledge of COVID-19 and nursing intention for COVID-19 patients. In addition, among the sub-factors of nursing professionalism, the only factor influencing nursing intention for COVID-19 patients was professionalism of nursing, which could explain approximately 6.0% of variance in nursing intention for COVID-19 patients (β=.58, p=.049).Conclusion: It is important to strengthen professionalism of nursing among various aspects of nursing professionalism in order to improve long-term care hospital nurses’ nursing intention for COVID-19 patients.
The Influence of Relational Bonds, Followership, and Occupational Stress on Nurses' Organizational Commitment
Authors: Choi Gipeum , Park Hyojung
Published: March 2023

Purpose: This descriptive survey-based study aimed to examine the degree of relational bonds, followership, occupational stress, and organizational commitment in nurses and to identify factors influencing organizational commitment.Methods: The research was conducted among 181 nurses who had more than 1 year of experience at a general hospital with 700 beds in Seoul. Relational bonds, followership, occupational stress, and organizational commitment were measured using a structured questionnaire. Data were collected from June 3 to June 28, 2021.Results: Relational bonds, followership, and occupational stress showed significant correlations with organizational commitment. Relational bonds (r=.45, p<.001) and followership (r=.55, p<.001) had positive correlations with organizational commitment, and occupational stress (r=-.44, p<.001) had a negative correlation with organizational commitment. Followership (β=.43, p<.001) and occupational stress (β=-.32, p<.001) were identified as factors affecting organizational commitment, and the total explanatory power was 41%.Conclusion: The findings of this study show that effective strategies are needed to enhance followership and reduce occupational stress among nurses, which could improve nurses' organizational commitment.
The Validity and Reliability of Nursing Assessment Communication-Competence Scale for Clinical Nurses
Authors: Kim Hyojin , Oh Heeyoung
Published: March 2023

Purpose: The aim of this study was to evaluate the validity and reliability of the short-version for clinical nurses on a communication competence scale developed to measure the communication competence of clinical nurses.Methods: Data were collected from 396 clinical nurses who work at general hospitals in Korea. The original version of the Communication Ability Scale was shorted and content, construct, item-convergent/discriminant, convergent validity and also internal consistency and test-retest reliability were evaluated. Data were analyzed using exploratory and confirmatory factor analyses, Pearsons's correlation coefficient and Cronbach’s ⍺.Results: As a result of item analysis and exploratory factor analysis, 5 subscales and a total of 20 items were derived. The confirmatory factor analysis showed adequate model fit indices (Normed x2=1.88, RMR=.04, RMSEA=.07, GFI=.87, CFI=.90, TLI=.87, IFI=.89). The items convergence and discrimination validity were verified using the Average Variance Extracted (.50~.56), composite reliability (.76~.86) and Φ±2SE (.42~.99). Convergent validity was demonstrated using Korean Version of Self-Efficacy Questionnaire (r=.59, p<.001). Internal consistency reliability and test-retest reliability were found to be acceptable, as indicated by a Cronbach’s ⍺ of .65~.79 and an intra-class correlation coefficient of .82~.90. The validity and reliability of the scale were verified.Conclusion: The NACCS is a valid and reliable instrument for measuring communication competence for clinical nurses in Korea.
Validation of the PAINAD-K Scale for Nonverbal Pain Assessment in the Post Anesthesia Care Unit
Authors: Kang Hyemin , Uhm Ju-Yeon
Published: March 2023

Purpose: This study aimed to verify the reliability and validity of the Pain Assessment in Advanced Dementia Scale-Korean version (PAINAD-K) for assessing pain in patients unable to communicate in the post-anesthesia care unit (PACU).Methods: The study included 205 patients, aged >65 years, admitted to the PACU following general surgery. Reliability was assessed using Cronbach’s ⍺, test-retest, intraclass correlation coefficient (ICC), and kappa coefficients. Criterion validity was evaluated with concurrent validity, and receiver operating characteristic (ROC) analysis assessed sensitivity and specificity. Convergent and discriminant validity were analyzed to verify the construct of the scale.Results: The Cronbach’s ⍺, ICC, and test-retest correlation values were .78, .95~.98, and .56~.74, respectively, indicating good reliability. At baseline, the correlation coefficient was .93 with the Algoplus scale in the non-communicative group and .82 with the numeric rating scale in the communicative group. Further establishing criterion validity in the nonverbal group, the area under the ROC curve was 0.995 (sensitivity=98.4%, specificity=97.5%) at baseline and .95 (sensitivity=96.4%, specificity=93.2%) at the 30-minute follow-up. The correlation coefficient with the Critical-Care Pain Observation Tool was .69~.96, showing convergent validity, and there was a significant interaction effect between time and group, indicating discriminant validity.Conclusion: The study findings suggest that the PAINAD-K is a psychometrically valid scale for assessing pain in PACU patients aged >65 years who are unable to communicate.
The Trousers and Research Methodology for Oppressive Design
Authors:   Bezalel Academy of Arts and Design, Bartal Ory
Published: March 2023

As clothing represents social, political, and performative values pertaining to gender, it is not surprising that they also serve as oppressive designed objects. One of the most significant symbols of gender power relations were the trousers that women were banned from wearing in the West as a daily fashion item until the second half of the 20th century. This article presents the history of trousers via a new research methodology for studying oppressive design. This methodology is built on Michel Foucault’s approach to genealogical research and Bruno Latour’s ideas about the social agency of objects. Just as Foucault revealed the history of norms, ideas, discourses, and values, which are abstract yet powerful entities, this methodology focuses on identifying the moment in which oppressive objects first entered into daily common use, becoming a new natural and oppressive ‘truth’ that shaped the worldview of its users. This approach builds on Latour’s argument that objects serve as mediating devices of values and discourses between individuals, and the idea that genealogical research concerning their use might expose their socio-historical function and powerful involvement in shaping and policing power relations over time.
Pluriversal Spaces for Decolonizing Design: Exploring Decolonial Directions for Participatory Design
Authors:   Umeå Universitet, Baroncelli Torretta Nicholas , Reitsma Lizette ,   Malmö Universitet, Hillgren Per-Anders ,   Malmö Universitet, van Ryneveld Tara Nair ,   Lund Universitet, Hansen Anne-Marie ,   Malmö Universitet, Castillo Muñoz Yénika ,   Independent Researcher
Published: March 2023

Decolonization is a situated effort as it relates to the relations of privilege, power, politics, and access (3P-A, in Albarrán González’s terms) between the people involved in design in relation to wider societies. This complexity creates certain challenges for how we can understand, learn about, and nurture decolonization in design towards pluriversality, since such decolonizing effort is based on the relationship between specific individuals and the collective. In this paper, we present and discuss the ‘River project’, a participatory space for decolonizing design, created for designers and practitioners to reflect on their own 3P-A as a way to create awareness of their own oppressive potential in design work. These joint reflections challenged ideas of participation and shaped learning processes between the participants, bringing to the foreground the importance of seeing and allowing for a plurality of life and work worlds to be brought together. We build on the learnings from this project to propose the notions of pluriversal participation, pluriversal presence, and pluriversal directionality, which can help nurture decolonizing designs towards pluriversality. We conclude by arguing that, for nurturing pluriversality through Participatory Design, participation, presence, and direction must be equally pluriversal.
Optimización de la rigidez equivalente en estructuras de uno y dos pisos sometidas a cargas de techos verdes
Authors: Contreras Bejarano O. , Ávila Hernández E. J. , Cepeda L. F.
Published: March 2023

Desde una perspectiva lineal y estática, el aumento de los desplazamientos de una estructura debido a la implementación de un techo verde podría ser contrarrestado con el incremento de la rigidez de la estructura. En este artículo se plantea el uso del algoritmo de evolución diferencial para optimizar la rigidez equivalente de una estructura de uno y dos pisos de altura, variando la carga de techo verde bajo el análisis de fuerza horizontal equivalente en la ciudad de Bogotá. A través del software MATLAB se programa y acopla el algoritmo de optimización y la metodología de análisis matricial de estructuras aporticadas tridimensionales sujetas al cumplimiento de la distorsión máxima de piso propuesta por la normativa de construcción colombiana. Un incremento máximo en la rigidez equivalente de 19.25% y 57.80% (estructuras de un piso), y de 11.12% y 32.97% (estructuras de dos pisos) para pesos de techos verdes de 100 kg/m2 y 300 kg/m2 respectivamente, es necesaria para cumplir requerimientos normativos. Se espera que esta investigación sirva como punto de partida para generar soluciones constructivas a la implementación de techos verdes en estructuras de uno y dos pisos en la ciudad de Bogotá.
Species Distribution of the Aerobic Bacterial Profile in Pyoderma with Special Reference to Antibiotic Susceptibility Pattern of the Isolates at a Tertiary Care Hospital of West Bengal, India
Authors: Dakua Koustab , Gupta Indrajit , Bishwas Abhilekha , Ghosh Shinjini , Kumar Simit , Chattopadhyay Shubhra
Published: March 2023

Introduction: Pyoderma is a common health problem characterised by pyogenic infections of the skin and its appendages. Though easily treatable, the condition is known for its chronicity, recurrence and other complications. Therefore, timely recognition and prompt bacterial diagnosis with antimicrobial sensitivity is imperative for the effective management and treatment of pyoderma. It is a common bacterial skin infection accounting for nearly 25% of patients attending Dermatology Outpatient Department (OPD) in India and other tropical countries. Aim: To determine the incidence of pyoderma in relation to age, sex and socio-economic status, to isolate and identify the common aerobic microbial pathogens associated with pyoderma prevalent in the community and antibiotic susceptibility pattern. Materials and Methods: An institutional based crosssectional observational study was conducted on 148 cases in Department of Microbiology, Rampurhat Government Medical College and Hospital, Burdwan, West Bengal, India, clinical features of suspected pyoderma for a period of 12 months from March 2021 to February 2022. Lesion swabs were collected and isolates were identified; antibiotic susceptibility testing was also performed according to Clinical and Laboratory Standards Institute (CLSI) guidelines using antibiotic discs. Descriptive data was presented as count and percentages. Results: Out of 148 samples collected 144 (97.3%) were culture positive while, rest 04 (2.7%) were culture negative. Overcrowding and low socio-economic group were closely related with pyoderma patients. Primary pyoderma (72.2%) cases were detected more than the secondary (27.8%) cases. Impetigo contagiosa (54%) were detected more among the primary pyoderma patients. High numbers (66.7%) of pyoderma were detected among children (0 to 10 years). The culture positive samples were more in male patients (68.7%) than in female patients (31.3%) and mostly detected from OPD. Out of 144 isolates, 89 (61.8%) were Staphylococcus aureus, 23 (15.9%) isolates were Coagulase Negative Staphylococci (CoNS) and 04 (2.8%) were Streptococcus pyogens. Further 06 (4.2%) isolates of Pseudomonas aeruginosa, 10 (6.9%) isolates of Escherichia coli and 08 (5.5%) isolates of Klebsiella pneumonia were found. Conclusion: The present study results suggest that the era of antibiotics has ushered in an unprecedented predominance of Staphylococcal rather than Streptococcal infections and other gram negative infections for pyoderma cases. Increasing incidence of methicillin, quinolones and amikacin resistance in Staphylococci and other gram negative isolates have limited treatment options. For this, a single infection like pyoderma is challenging in all the healthcare facility.
Ethno-botanical Survey of Plants Applied for the Treatment of Sexually Transmitted Diseases in the Sudan Savannah Region of Nigeria
Authors: Salami K. D. , Alao J. S. , Abubakar U. , Adam Fatima
Published: March 2023

This study aimed at the assessment of ethno-botanical plants applied by most the rural people as a therapy for Sexually Transmitted Diseases. Ethno-botanical study of medicinal plants was carried out from January 2023 to March 5, 2023 at Dutse, Local Government Dutse, Jigawa State, Nigeria. Purposive random sampling technique was used and one hundred and forty (140) structured questionnaires were administered to the respondents which were 40% of population (350). Two major markets sampled were: Tsilliya (Kiyawa) and Shuwarin market (Dutse). Descriptive statistics was used such as table, frequency and percentage. Results showed that majority of the respondents (32.14%) were within the age brackets of 21-30 and 63.57% were married, 63.57% had non-formal education while 53.57% of the respondents’ occupation was herb sellers. The prominent religion in the study area was Muslim (91.43%). A total of 26 plants observed, distributed into 15 families used in the management of eight (8) diseases. Combreataceae (4 species) and Legumenaceae (4 species) had the highest plants in the study area applied for various diseases treatment. Jatropha curcas (8) had the highest frequency followed by Securidaca longepedunculata (6). Gonorrhea is the most prominent disease (8) followed by virginal discharge (6) respectively. Result also showed that maceration had the highest percentage with the total of 68% followed by decoction method which contains 20% followed by infusion techniques which was 8%. Liquid extract was rated least with the total of 4%. Bark was mostly used (37.50%), roots (18.51%), whole plant (18.51%) and leaf (11.11%). It is therefore, necessary to recommend the preservation of the local knowledge of traditional medicine by appropriate identification, conservation, preparation application of the plant species.
Proximate Composition of Banana and Pineapple Wine Fermented by Meyerozyma guilliermondii and Pichia guilliermondii
Authors: Alabere A. , Adebayo-Olajide T. C.
Published: March 2023

Wine is an alcoholic beverage produced from juices of a variety of fruits by the fermentative action of microorganisms. There is a quest for alternative sources of must for wine fermentation, however, the proximate composition should be known to ascertain how nutritious it is. The study was thus aimed at determining the proximate composition of the wines fermented by Meyerozyma guilliermondii and Pichia guilliermondii. Two isolates identified as Meyerozyma guilliermondii strain 1621 and Pichia guilliermondii strain PAX-PAT 18S were used for the fermentation of the substrates obtained from a mixture of pineapple and banana pulp. The fermentation process was for 28 days, followed by a series of racking, clarification, and aging process which was for 2 months. The fermentation process comprised two setups: one was fermented by Meyerozyma guilliermondii strain 1621 and the other by Pichia guilliermondii strain PAX-PAT 18S. The process was monitored and the proximate analysis of the wines was ascertained. The wine produced by Meyerozyma guilliermondii strain 1621 had a moisture content of 82.56 %, ash content of 1.41 %, fat content of 0.08 %, protein content of 1.43%, and carbohydrate content of 9.77%. The wine produced by Pichia guilliermondii strain PAX-PAT 18S had a moisture content of 79.51%, ash content of 1.19%, fat content of 0.15%, protein content of 0.49%, and carbohydrate content of 10.49%. Although this study is not exhaustive, it shows that wines with good nutritional composition can be successfully produced using Meyerozyma guilliermondii strain 1621 and Pichia guilliermondii strain PAX-PAT 18S.
Casting the couch
Authors: Milton Chris , Malcolm Charles
Published: March 2023

Analysis occurs when the space of meeting becomes the place of encounter. This place is no ordinary place, it is a place dedicated to the analysand appropriating her/his own, authentic possibilities of being. The place and process which is analysis is evoked in several ways: by the presence of analyst and analysand, the 'rules' which guide the process and the ambiance of the setting. The last of these includes the use (or not} of the analytic couch.
Drawing on qualitative research into several analysts' lived experience of having an analys and use the couch this paper discusses the role of the couch in constituting intersubjective privacy and some of the ways in which the couch contributes to a particular way ofbeing-together-with, bodily attunement, the emergence of an analytic third, and reverie.
The relevance of play to the teaching and learning of psychotherapy
Authors: McDermott Anne
Published: March 2023

I draw on my backgrounds as a kindergarten teacher, psychotherapist and supervisor to examine how play is relevant in the teaching and learning of psychotherapy. Specifically, the role of play in supervision is explored. Within the supervisory relationship, I examine the kinds of learning spaces that enhance or impede development. This paper is an expanded version of a paper published in Psychotherapy in Australia (2005) 12(1), 28-31.
Perforation During Gynecological Procedures
Authors: Richards Elliott G. , Rehmer Jenna M. , Falcone Tommaso
Published: March 2023

This article in the Women’s Health series discusses uterine perforation occurring during gynecological procedures, including prevention, identification of risk factors, recognition, management, and long-term outcomes.
Association of BRAF Variants With Disease Characteristics, Prognosis, and Targeted Therapy Response in Intrahepatic Cholangiocarcinoma
Authors: Xin Hao-Yang , Sun Rong-Qi , Zou Ji-Xue , Wang Peng-Cheng , Wang Jia-Yin , Ye Yu-Hang , Liu Kai-Xuan , Hu Zhi-Qiang , Zhou Zheng-Jun , Fan Jia , Zhou Jian , Zhou Shao-Lai
Published: March 2023

Importance

BRAF variants are associated with tumor progression; however, the prevalence of BRAF variant subtypes and their association with disease characteristics, prognosis, and targeted therapy response in patients with intrahepatic cholangiocarcinoma (ICC) are largely unknown.

Objective

To explore the association of BRAF variant subtypes with disease characteristics, prognosis, and targeted therapy response in patients with ICC.

Design, Setting, and Participants

In this cohort study, 1175 patients who underwent curative resection for ICC from January 1, 2009, through December 31, 2017, were evaluated at a single hospital in China. Whole-exome sequencing, targeted sequencing, and Sanger sequencing were performed to identify BRAF variants. The Kaplan-Meier method and log-rank test were used to compare overall survival (OS) and disease-free survival (DFS). Univariate and multivariate analyses were performed using Cox proportional hazards regression. Associations between BRAF variants and targeted therapy response were tested in 6 BRAF-variant, patient-derived organoid lines and in 3 of the patient donors of those lines. Data were analyzed from June 1, 2021, to March 15, 2022.

Interventions

Hepatectomy in patients with ICC.

Main Outcomes and Measures

The association of BRAF variant subtypes with OS and DFS.

Results

Of 1175 patients with ICC, the mean (SD) age was 59.4 (10.4) years and 701 (59.7%) were men. A total of 20 different subtypes of BRAF somatic variance affecting 49 patients (4.2%) were identified; V600E was the most frequent allele in this cohort, accounting for 27% of the identified BRAF variants, followed by K601E (14%), D594G (12%), and N581S (6%). Compared with patients with non-V600E BRAF variants, patients with BRAF V600E variants were more likely to have large tumor size (10 of 13 [77%] vs 12 of 36 [33%]; P = .007), multiple tumors (7 of 13 [54%] vs 8 of 36 [22%]; P = .04), and more vascular/bile duct invasion (7 of 13 [54%] vs 8 of 36 [22%]; P = .04). Multivariate analysis revealed that BRAF V600E variants, but not overall BRAF variants or non-V600E BRAF variants, were associated with poor OS (hazard ratio [HR], 1.87; 95% CI, 1.05-3.33; P = .03) and DFS (HR, 1.66; 95% CI, 1.03-2.97; P = .04). There were also broad differences among organoids with different BRAF variant subtypes in sensitivity to BRAF or MEK inhibitors.

Conclusions and Relevance

The findings of this cohort study suggest that there are broad differences among organoids with different BRAF variant subtypes in sensitivity to BRAF or MEK inhibitors. Identifying and classifying BRAF variants may be able to help guide precise treatment for patients with ICC.
Obesity‐induced oxidative stress and mitochondrial dysfunction negatively affect sperm quality
Authors: Jing Jia , Peng Yuanhong , Fan Weimin , Han Siyang , Peng Qihua , Xue Chunran , Qin Xinran , Liu Yue , Ding Zhide
Published: March 2023

Obesity is a systemic metabolic disease that can induce male infertility or subfertility through oxidative stress. The aim of this study was to determine how obesity impairs sperm mitochondrial structural integrity and function, and reduces sperm quality in both overweight/obese men and mice on a high‐fat diet (HFD). Mice fed the HFD demonstrated higher body weight and increased abdominal fat content than those fed the control diet. Such effects accompanied the decline in antioxidant enzymes, such as glutathione peroxidase (GPX) and catalase and superoxide dismutase (SOD) in testicular and epidydimal tissues. Moreover, malondialdehyde (MDA) content significantly increased in sera. Mature sperm in HFD mice demonstrated higher oxidative stress, including increased mitochondrial reactive oxygen species (ROS) levels and decreased protein expression of GPX1, which may impair mitochondrial structural integrity and reduce mitochondrial membrane potential (MMP) and ATP production. Moreover, cyclic AMPK phosphorylation status increased, whereas sperm motility declined in the HFD mice. Clinical studies demonstrated that being overweight/obese reduced SOD enzyme activity in the seminal plasma and increased ROS in sperm, accompanied by lower MMP and low‐quality sperm. Furthermore, ATP content in the sperm was negatively correlated with increases in the BMI of all clinical subjects. In conclusion, our results suggest that excessive fat intake had similar disruptive effects on sperm mitochondrial structure and function, as well as oxidative stress levels in humans and mice, which in turn induced lower sperm motility. This agreement strengthens the notion that fat‐induced increases in ROS and impaired mitochondrial function contribute to male subfertility.
Coherence of child maltreatment narratives by emerging adults: How does child maltreatment affect how we share our story?
Authors: Fishere Mariam , Habermas Tilmann
Published: March 2023

Individuals narrate stories to explain how they became who they are, forming their own narrative identity. Highly disruptive experiences such as child maltreatment (CM) may be challenging to integrate into the life story and even to narrate coherently just by themselves. To test these potential effects, we divided a total of 171 students at an English‐speaking university in Egypt into two groups, those with a history of CM and those who experienced none. We compared narratives of four different types of emotional childhood experiences. Narratives of CM were no less coherent than narratives of most stressful childhood experiences in the control group, but were more integrated into the life story, which in turn predicted symptoms of posttraumatic stress disorder (PTSD). The present study contributes to the literature by confirming that there is no evidence for a lack of coherence among adults' CM narratives, and by highlighting the association between the centrality of the traumatic event in one's life and PTSD symptoms.
The Electroless Monolayer and Duplex Ni–B and Ni–P Coatings for 316L Stainless Steel in Synergistic Combination of Mechanical (Wear) and Chemical (Corrosion) Processes
Authors: Mindivan Ferda , Mindivan Harun , Bayram Ali
Published: March 2023

Conventional processes are performed to improve the low hardness and low wear resistance properties of 316L steel, but these processes generally decrease the corrosion resistance. Electroless nickel alloy coatings provide a hard, wear‐resistant, and corrosion‐resistant surface. Thus, the present study aims to investigate the wear, corrosion, and tribocorrosion behaviors of monolayer and duplex coatings with nickel–boron (Ni–B) and nickel–phospore (Ni–P) on 316L steel in comparison with 316L steel in dry sliding and 0.9 wt% NaCl solution environments. It is determined that the coatings have a mixture of crystal and amorphous structures, the interfaces on the 316L are uniform, and the compatibility between the layers is good. The Ni–B, Ni–P/Ni–B, and Ni–B/Ni–P coatings are 2.3, 2.06, and 1.6 times as hard as the 316L, respectively. The wear rates of Ni–B, Ni–P/Ni–B, and Ni–B/Ni–P coatings show decrease by 99.3%, 92.5%, and 99.1% in the dry‐sliding condition and by 98.5%, 30.1%, and 19.1% in the tribocorrosion condition compared with that of 316L, respectively. It is observed that the monolayer Ni–B coating exhibits superior hardness, a higher contact angle, low electrical conductivity, and better tribological performance in both dry sliding and tribocorrosion conditions compared to the 316L and duplex coatings.
Building Highly Light‐Harvesting Near‐Infrared AIEgens Using Triazole‐Based Luminescent Core for Improved Intravital Afterglow Imaging
Authors: Li Jisen , Zhang Guo‐Qiang , Zhang Yufan , Tang Youhong , Ding Dan , Li Wen , Liu Qian
Published: March 2023

Development of the powerful building block is of great significance to construct materials with advanced properties. Herein, for the first time, a triazole‐based luminescent core with balanced twist and conjugation is reported, which is explored to construct a D‐A near‐infrared (NIR) aggregation‐induced emission luminogens (TPT‐DCM) with high molar extinction coefficient, good brightness and excellent reactive oxygen species generation rate. These features enable it to function as a nanoprobe with ultralong NIR afterglow luminescence (up to 20 days) and ultrahigh tumor‐to‐liver signal ratio (up to 187) for in vivo deep‐tissue afterglow imaging (with depth reaching 1.6 cm), in combination with chemiluminescence resonance energy transfer aided by active Schaap's dioxetane. Moreover, thanks to the excellent properties of the nanoprobe, the afterglow imaging‐guided surgery navigation can be successfully conduced to remove the tumors especially with tiny size of < 1 mm. This is particularly useful to eliminate tumor residuals and prevent the cancer recurrence after surgery.
Low‐Valent Manganese Atoms Stabilized on Ceria for Nitrous Oxide Synthesis
Authors: Surin Ivan , Tang Zhenchen , Geiger Julian , Damir Suyash , Eliasson Henrik , Agrachev Mikhail , Krumeich Frank , Mitchell Sharon , Kondratenko Vita A. , Kondratenko Evgenii V. , Jeschke Gunnar , Erni Rolf , López Núria , Pérez‐Ramírez Javier
Published: March 2023

Nitrous oxide, N2O, exhibits unique reactivity in oxidation catalysis, but the high manufacturing costs limit its prospective uses. Direct oxidation of ammonia, NH3, to N2O can ameliorate this issue but its implementation is thwarted by suboptimal catalyst selectivity and stability, and the lack of established structure–performance relationships. Systematic and controlled material nanostructuring offers an innovative approach for advancement in catalyst design. Herein low‐valent manganese atoms stabilized on ceria, CeO2, are discovered as the first stable catalyst for NH3 oxidation to N2O, exhibiting two‐fold higher productivity than the state‐of‐the‐art. Detailed mechanistic, computational and kinetic studies reveal CeO2 as the mediator of oxygen supply, while undercoordinated manganese species activate O2 and facilitate N2O evolution via NN bond formation between nitroxyl, HNO, intermediates. Synthesis via simple impregnation of a small metal quantity (1 wt%) predominantly generates isolated manganese sites, while full atomic dispersion is achieved upon redispersion of sporadic oxide nanoparticles during reaction, as confirmed by advanced microscopic analysis and electron paramagnetic resonance spectroscopy. Subsequently, manganese speciation is maintained, and no deactivation is observed over 70 h on stream. CeO2‐supported isolated transition metals emerge as a novel class of materials for N2O production, encouraging future studies to evaluate their potential in selective catalytic oxidations at large.
PIP2 Alteration Caused by Elastic Modulus and Tropism of Electrospun Scaffolds Facilitates Altered BMSCs Proliferation and Differentiation
Authors: Gao Chen , Lai Yulin , Cheng Liang , Cheng Yifan , Miao Anqi , Chen Jialong , Yang Runhuai , Xiong Fei
Published: March 2023

Aligned submicron fibers have played an essential role in inducing stem cell proliferation and differentiation. In this study, it is aimed to identify the differential causes of stem cell proliferation and differentiation between bone marrow mesenchymal stem cells (BMSCs) on aligned‐random fibers with different elastic modulus, and to change the differential levels through a regulatory mechanism mediated by B‐cell lymphoma 6 protein(BCL‐6) and miRNA‐126‐5p(miR‐126‐5p). The results showed that phosphatidylinositol(4,5)bisphosphate alterations are found in the aligned fibers compared with the random fibers, which has a regular and oriented structure, excellent cytocompatibility, regular cytoskeleton, and high differentiation potential. The same trend is actual for the aligned fibers with a lower elastic modulus. The level of proliferative differentiation genes in cells is altered by BCL‐6 and miR‐126‐5p mediated regulatory mechanisms to make the cell distribution nearly consistent with the cell state on low elastic modulus aligned fibers. This work demonstrates the reason for the difference of cells between the two kinds of fibers and on fibers with different elastic modulus. These findings provide more insights for understanding the gene‐level regulation of cell growth in tissue engineering.
Compromising Charge Generation and Recombination of Organic Photovoltaics with Mixed Diluent Strategy for Certified 19.4% Efficiency
Authors: Chen Tianyi , Li Shuixing , Li Yaokai , Chen Zeng , Wu Haotian , Lin Yi , Gao Yuan , Wang Mengting , Ding Guanyu , Min Jie , Ma Zaifei , Zhu Haiming , Zuo Lijian , Chen Hongzheng
Published: March 2023

The ternary blend is demonstrated as an effective strategy to promote the device performance of organic photovoltaics (OPVs) due to the dilution effect. While the compromise between the charge generation and recombination remains a challenge. Here, a mixed diluent strategy for further improving the device efficiency of OPV is proposed. Specifically, the high‐performance OPV system with a polymer donor, i.e., PM6, and a nonfullerene acceptor (NFA), i.e., BTP‐eC9, is diluted by the mixed diluents, which involve a high bandgap NFA of BTP‐S17 and a low bandgap NFA of BTP‐S16 (similar with that of the BTP‐eC9). The BTP‐S17 of better miscibility with BTP‐eC9 can dramatically enhance the open‐circuit voltage (VOC), while the BTP‐S16 maximizes the charge generation or the short‐circuit current density (JSC). The interplay of BTP‐17 and BTP‐S16 enables better compromise between charge generation and recombination, thus leading to a high device performance of 19.76% (certified 19.41%), which is the best among single‐junction OPVs. Further analysis on carrier dynamics validates the efficacy of mixed diluents for balancing charge generation and recombination, which can be further attributed to the more diverse energetic landscapes and improved morphology. Therefore, this work provides an effective strategy for high‐performance OPV for further commercialization.
Inkjet Printing Bio‐Inspired Electrochromic Pixels
Authors: Sullivan Patrick A. , Wilson Daniel J. , Vallon Matthew , Bower Duncan Q. , Deravi Leila F.
Published: March 2023

In this report the design, fabrication, and testing of inkjet‐printed electrochromic pixels (ECPs) incorporating the biochrome, xanthommatin (Xa) as programmable display units is described. As a redox sensitive chromophore, Xa is present in some species as a physiological indicator with red (reduced) or yellow (oxidized) colors associated with different behavioral or developmental stages. These features have been recently leveraged in some materials applications, illustrating a bio‐inspired design solution to color‐changing sensors and displays. This paper describes an extension of these applications to print individually addressable ECPs that can be processed in a mild annealing step to introduce localized conductivity on initially nonconductive substrates. When formulated together with a poly(3,4‐ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) carrier ink, an addition of 0.19 wt% Xa is enough to generate dynamic ECPs which can be batch printed as lateral electrodes on any substrate to serve as both conductors and display units across electrically isolated boundaries. Application of low potentials triggers reversible color changes that span the red/yellow color space and can cycle for days. These results represent an important step towards the incorporation of alternative active materials like Xa to manufacture and scale low‐power, color‐changing pixels and patterns.
Swelling‐Induced Structural Transformation Strategy: Controllable Synthesis of 2D Porous Polypyrrole/MXene Heterostructures with Tunable Pore Structures
Authors: Liu Zhilin , Zhang Rui , Xiong Hailong , Zhang Liangliang , Li Junzhi , Wang Luoqi , Qiao Zhen‐An
Published: March 2023

2D porous polymers have received great attention in many fields. Particularly, 2D porous polymers are considered as potential electrode materials for supercapacitors. However, the controllable synthesis of 2D porous polymers is not only faced with the difficulty in precisely controlling the reaction dynamics but is also challenged by the complex pore structure fabrication. In this paper, a swelling‐induced structural transformation strategy for the construction of 2D porous polypyrrole/MXene heterostructures (2D porous PPy/MXene) is proposed. The obtained 2D porous PPy/MXene samples own similar chemical compositions with adjusted pore structures (cylindrical mesostructure, spherical mesostructure, and spherical macrostructure), high specific surface areas (≈129–188 m2 g−1), and tunable pore sizes (≈7.8–52 nm). Due to the fast transport and diffusion of electrolytes and more electrode/electrolyte interface offered by cylindrical mesopores with high specific surface areas, the 2D porous PPy/MXene shows superior supercapacitor performances. This work sheds light on the effect of pore structures on supercapacitor performances of 2D porous polymers, and also provides clues for the fabrication of 2D porous polymers with tailorable pore structures as high‐performance energy storage materials.
Broadband Nonlinear Photonics in Zeolite‐Based Selenium Chains
Authors: Sun Lang , Sun Dalin , Xu Xintong , Chu Shaowen , Chen YeWang , Wang Meng , Lu Juan , Chen Jiaqi , Ruan Shuangchen
Published: March 2023

As one of the 1D nanomaterials, Se nanochains have been widely used in photoelectric devices, such as photodetectors, battery devices, and optical sensors due to their excellent photoelectric properties. Among them, ultrafast photonics has attracted much attention due to its wide applications; for instance, environmental monitoring, material processing, biomedical imaging, nonlinear optical generation, and free‐space optical communication. Herein, Se nanochains are fabricated by trapping Se species in the circular 1D channel of AlPO4‐5 (AFI) crystal, and the characteristics of Se@AFI are systemically investigated. In addition, the AFI single crystal loaded with Se nanochains in the channel is successfully prepared as a saturable absorber in the fiber laser. The mode‐locking pulse at 1.5 and 1 μm with the repetition rate of 13.16 and 14.74 MHz can be obtained by integrating the Se@AFI composite material in the cavity. The signal‐to‐noise ratios are both over 70 dB, suggesting the high operational stability of the pulses. These results indicate that Se@AFI has good potential as saturable absorber material with a broad wavelength range for ultrafast pulsed fiber lasers. Our results will also provide a new idea for designing air‐stable ultrafast photonic devices and other composite materials in various fields.
Broadband White‐Light‐Emitting Electrochemical Cells
Authors: Adranno Brando , Tang Shi , Paterlini Veronica , Smetana Volodymyr , Renier Olivier , Bousrez Guillaume , Edman Ludvig , Mudring Anja-Verena
Published: March 2023

Emerging organic light‐emitting devices, such as light‐emitting electrochemical cells (LECs), offer a multitude of advantages but currently suffer from that most efficient phosphorescent emitters are based on expensive and rare metals. Herein, it is demonstrated that a rare metal‐free salt, bis(benzyltriphenylphosphonium)tetrabromidomanganate(II) ([Ph3PBn]2[MnBr4]), can function as the phosphorescent emitter in an LEC, and that a careful device design results in the fact that such a rare metal‐free phosphorescent LEC delivers broadband white emission with a high color rendering index (CRI) of 89. It is further shown that broadband emission is effectuated by an electric‐field‐driven structural transformation of the original green‐light emitter structure into a red‐emitting structure.
Whither social media and clinical genetics?
Authors: Gunter Chris , Solomon Benjamin D.
Published: March 2023

Social media has become ubiquitous in daily life, and increasingly impacts medical and scientific fields, including related to clinical genetics. Recent events have led to questions about the use of certain social media platforms, as well as social media more generally. We discuss these considerations, including alternative and emerging platforms that can offer forums for the clinical genetics and related communities.