bluuebunny/crossref_metadata_2025 huggingface dataset:
GmSWEET29 and Paralog GmSWEET34 Are Differentially Expressed between Soybeans Grown in Eastern and Western Canada
Authors: Hooker Julia C. , Nissan Nour , Luckert Doris , Zapata Gerardo , Hou Anfu , Mohr Ramona M. , Glenn Aaron J. , Barlow Brent , Daba Ketema A. , Warkentin Thomas D. , Lefebvre François , Golshani Ashkan , Cober Elroy R. , Samanfar Bahram
Published: September 2022

Over the past two decades soybeans grown in western Canada have persistently had lower seed protein than those grown in eastern Canada. To understand the discrepancy in seed protein content between eastern- and western-grown soybeans, RNA-seq and differential expression analysis have been investigated. Ten soybean genotypes, ranging from low to high in seed protein content, were grown in four locations across eastern (Ottawa) and western (Morden, Brandon, and Saskatoon) Canada. Differential expression analysis revealed 34 differentially expressed genes encoding Glycine max Sugars Will Eventually be Exported Transporters (GmSWEETs), including paralogs GmSWEET29 and GmSWEET34 (AtSWEET2 homologs) that were consistently upregulated across all ten genotypes in each of the western locations over three years. GmSWEET29 and GmSWEET34 are likely candidates underlying the lower seed protein content of western soybeans. GmSWEET20 (AtSWEET12 homolog) was downregulated in the western locations and may also play a role in lower seed protein content. These findings are valuable for improving soybean agriculture in western growing regions, establishing more strategic and efficient agricultural practices.
Membrane Localized GbTMEM214s Participate in Modulating Cotton Resistance to Verticillium Wilt
Authors: Zhao Jun , Xu Jianwen , Wang Yueping , Liu Jianguang , Dong Chengguang , Zhao Liang , Ai Nijiang , Xu Zhenzhen , Guo Qi , Feng Guoli , Xu Peng , Cheng Junling , Wang Xin , Wang Juan , Xiao Songhua
Published: September 2022

Verticillium wilt (VW) is a soil-borne fungal disease caused by Verticillium dahliae Kleb, which leads to serious damage to cotton production annually in the world. In our previous study, a transmembrane protein 214 protein (TMEM214) gene associated with VW resistance was map-based cloned from Gossypium barbadense (G. barbadense). TMEM214 proteins are a kind of transmembrane protein, but their function in plants is rarely studied. To reveal the function of TMEM214s in VW resistance, all six TMEM214s were cloned from G. barbadense in this study. These genes were named as GbTMEM214-1_A/D, GbTMEM214-4_A/D and GbTMEM214-7_A/D, according to their location on the chromosomes. The encoded proteins are all located on the cell membrane. TMEM214 genes were all induced with Verticillium dahliae inoculation and showed significant differences between resistant and susceptible varieties, but the expression patterns of GbTMEM214s under different hormone treatments were significantly different. Virus-induced gene silencing analysis showed the resistance to VW of GbTMEM214s-silenced lines decreased significantly, which further proves the important role of GbTMEM214s in the resistance to Verticillium dahliae. Our study provides an insight into the involvement of GbTMEM214s in VW resistance, which was helpful to better understand the disease-resistance mechanism of plants.
Crosstalk of Cytokinin with Ethylene and Auxin for Cell Elongation Inhibition and Boron Transport in Arabidopsis Primary Root under Boron Deficiency
Authors: Herrera-Rodríguez María Begoña , Camacho-Cristóbal Juan José , Barrero-Rodríguez Rafael , Rexach Jesús , Navarro-Gochicoa María Teresa , González-Fontes Agustín
Published: September 2022

Several studies have shown the role of phytohormones in the regulation of root growth of Arabidopsis plants under boron (B) deficiency. Ethylene and auxin play an important role in the control of Arabidopsis primary root cell elongation under short-term B deprivation, whereas cytokinins regulate root growth inhibition under B deficiency by controlling meristem cell proliferation. In this work, we study the possible interaction among cytokinin, ethylene, and auxin in the primary root response to B-deprivation treatment, as well as their possible role in B uptake and transport. Wild type (WT) and two mutants related to auxin and ethylene (aux1 and acs11) Arabidopsis plants were grown in control (10 µM B) or B starvation (0 µM B) treatment, in the absence or presence of trans-zeatin, and their primary root growth was analyzed. The possible interaction between these hormones was also studied by analyzing AUX1 gene expression in the acs11 mutant and ACS11 gene expression in the aux1 mutant. The GUS reporter lines ARR5::GUS, IAA2::GUS, and EBS::GUS were used to observe changes in cytokinin, auxin, and ethylene levels in the root, respectively. The results of this work suggest that cytokinin inhibits root cell elongation under B deficiency through two different mechanisms: (i) an ethylene-dependent mechanism through increased expression of the ACS11 gene, which would lead to increased ethylene in the root, and (ii) an ethylene-independent mechanism through decreased expression of the AUX1 gene, which alters auxin signaling in the meristematic and elongation zones and stele. We also report that changes in the expression of several B transporters occur in response to auxin, ethylene, and cytokinin that may affect the plant B content.
The Upper Range Limit of Alien Plants Is Not in Equilibrium with Climate in the Andes of Central Chile
Authors: Goncalves Estefany , Herrera Ileana , Alexander Jake , Duarte Milen , Cavieres Lohengrin A. , Morales-Salinas Luis , Bustamante Ramiro O.
Published: September 2022

Alien plant species are colonizing high-elevation areas along roadsides. In this study, we evaluated whether the distributions of alien plants in the central Chilean mountains have reached climatic equilibrium (i.e., upper distribution limits consistent with their climatic requirements). First, we evaluated whether the upper elevational limits of alien plants changed between 2008 and 2018 based on the Mountain Invasion Research Network (MIREN) database. Second, we compared the observed upper elevational limits with the upper limits predicted by each species’ global climatic niche. On average across species, the upper elevation limit did not change between 2008 and 2018. However, most species maintained the same limit or shifted downward, while only 23% of the species shifted upwards. This lack of change does not mean that the species’ distributions are in equilibrium with the climate, because the observed upper limit was lower than the limit predicted by the global niche model for 87% of species. Our results suggest that alien species in this study region may not only be climate-limited, but could also be limited by other local-scale factors, such as seed dispersal, intermittent disturbance rates, soil type and biotic interactions.
Distribution and Biodiversity of Seed-Borne Pathogenic and Toxigenic Fungi of Maize in Egypt and Their Correlations with Weather Variables
Authors: Shabana Yasser M. , Ghoneem Khalid M. , Rashad Younes M. , Arafat Nehal S. , Fitt Bruce D. L. , Richard Benjamin , Qi Aiming
Published: September 2022

Studies of the biodiversity of plant pathogenic and toxigenic fungi are attracting great attention to improve the predictability of their epidemics and the development of their control programs. Two hundred maize grain samples were gathered from 25 maize-growing governorates in Egypt and 189 samples were processed for the isolation and identification of seed-borne fungal microbiome. Twenty-six fungal genera comprising 42 species were identified according to their morphological characteristics and ITS DNA sequence analysis. Occurrence and biodiversity indicators of these fungal species were calculated. Ustilago maydis, Alternaria alternata, Aspergillus flavus, A. niger, Penicillium spp., Cladosporium spp. and Fusarium verticillioides were the highly frequent (>90% for each), recording the highest relative abundance (˃50%). Al-Menia governorate showed the highest species diversity and richness, followed by Sohag, Al-Nobaria and New Valley governorates. Correlations of 18 fungal species with temperature, relative humidity, precipitation, wind speed, and solar radiation were analyzed using canonical correspondence analysis. Results showed that relative humidity, temperature, and wind speed, respectively, were the most impactful weather variables. However, the occurrence and distribution of these fungi were not clearly grouped into the distinctive climatic regions in which maize crops are grown. Monitoring the occurrence and distribution of the fungal pathogens of maize grains in Egypt will play an important role in predicting their outbreaks and developing appropriate future management strategies. The findings in this study may be useful to other maize-growing countries that have similar climatic conditions.
Molecular Dynamics Simulations of the Interactions between a Hydrolyzed Polyacrylamide with the Face and Edge Surfaces of Molybdenite
Authors: Echeverry-Vargas Luver , Estrada Darwin , Gutierrez Leopoldo
Published: September 2022

Process water used in mineral processing operations corresponds to water recovered from the thickeners and tailings dams, containing residual reagents such as hydrolyzed polyacrylamides (HPAMs). These polymers depress the flotation of different minerals, and their effect on molybdenite has been experimentally demonstrated. The objective of this work was to study the interactions between a segment of a HPAM with the face and edge of molybdenite. The sigma profile, the radial distribution functions of the HPAM, and the orientation and atomic density profiles of water molecules on the face and edge surfaces of molybdenite were calculated. The results obtained from molecular dynamics simulations showed that the interactions between the HPAM and molybdenite are mainly explained by the interactions of the amide group with the faces and edges of the mineral. Molecular dynamics simulations also showed that the HPAM molecule rearranges in such a way that the amide group moves towards the molybdenite face or edge, and the carboxylate group moves away from the mineral surface. The results obtained in the simulations showed that the interactions of the HPAM with the molybdenite edge are slightly stronger than the interaction of this molecule with the mineral face. Simulations demonstrated that the presence of the sodium and hydroxide ions reduces the concentration of HPAM around the face and edge surfaces, which is expected to affect HPAM adsorption on molybdenite. The conclusions obtained through molecular dynamics simulations are in line with the results obtained in previous studies carried out at a macroscopic scale, which reported that HPAMs adsorb onto molybdenite particles and reduce their hydrophobicity.
Rheological Method for Determining the Molecular Weight of Collagen Gels by Using a Machine Learning Technique
Authors: Núñez Carrero Karina C. , Velasco-Merino Cristian , Asensio María , Guerrero Julia , Merino Juan Carlos
Published: September 2022

This article presents, for the first time, the results of applying the rheological technique to measure the molecular weights (Mw) and their distributions (MwD) of highly hierarchical biomolecules, such as non-hydrolyzed collagen gels. Due to the high viscosity of the studied gels, the effect of the concentrations on the rheological tests was investigated. In addition, because these materials are highly sensitive to denaturation and degradation under mechanical stress and temperatures close to 40 °C, when frequency sweeps were applied, a mathematical adjustment of the data by machine learning techniques (artificial intelligence tools) was designed and implemented. Using the proposed method, collagen fibers of Mw close to 600 kDa were identified. To validate the proposed method, lower Mw species were obtained and characterized by both the proposed rheological method and traditional measurement techniques, such as chromatography and electrophoresis. The results of the tests confirmed the validity of the proposed method. It is a simple technique for obtaining more microstructural information on these biomolecules and, in turn, facilitating the design of new structural biomaterials with greater added value.
Slurry Homopolymerization of Ethylene Using Thermostable α-Diimine Nickel Catalysts Covalently Linked to Silica Supports via Substituents on Acenaphthequinone-Backbone
Authors: Zong Kening , Hou Yanhui , Zhao Xiaobei , Sun Yali , Liu Binyuan , Yang Min
Published: September 2022

Four supported α-diimine nickel(II) catalysts covalently linked to silica via hydroxyl functionality on α-diimine acenaphthequinone-backbone were prepared and used in slurry polymerizations of ethylene to produce branched polyethylenes. The catalytic activities of these still reached 106 g/molNi·h at 70 °C. The life of the supported catalyst is prolonged, as can be seen from the kinetic profile. The molecular weight of the polyethylene obtained by the 955 silica gel supported catalyst was higher than that obtained by the 2408D silica gel supported catalyst. The melting points of polyethylene obtained by the supported catalysts S-C1-a/b are all above 110 °C. Compared with the homogeneous catalyst, the branching numbers of the polyethylenes obtained by the supported catalysts S-C1-a/b is significantly lower. The polyethylenes obtained by supported catalyst S-C1-a/b at 30–50 °C are free-flowing particles, which is obviously better than the rubber-like cluster polymer obtained from homogeneous catalyst.
Colorimetric Freshness Indicator Based on Cellulose Nanocrystal–Silver Nanoparticle Composite for Intelligent Food Packaging
Authors: Kwon Seongyoung , Ko Seonghyuk
Published: September 2022

In this study, a colorimetric freshness indicator based on cellulose nanocrystal-silver nanoparticles (CNC-AgNPs) was successfully fabricated to offer a convenient approach for monitoring the quality of packaged food. AgNPs were directly synthesized and embedded in CNC via a one-pot hydrothermal green synthesis, and CNC-AgNP composited indicator films were prepared using a simple casting method. The AgNPs obtained were confirmed by UV-Vis diffuse reflectance spectroscopy and X-ray diffraction. The ability of the as-prepared CNC-AgNP film to indicate food quality was assessed in terms of the intensity of its color change when in contact with spoilage gases from chicken breast. The CNC-AgNP films initially exhibited a yellowish to dark wine-red color depending on the amount of AgNPs involved. They gradually turned colorless and subsequently to metallic grey. This transition is attributed to the reaction of AgNPs and hydrogen sulfide (H2S), which alters the surface plasmon resonance of AgNPs. Consequently, the color change was suitably discernible to the human eye, implying that the CNC-AgNP composite is a highly effective colorimetric freshness indicator. It can potentially serve as an accurate and irreversible food quality indicator in intelligent packaging during distribution or storage of products that emit hydrogen sulfide when deteriorating, such as poultry products or broccoli.
Utilisation of Paunch Waste as a Natural Fibre in Biocomposites
Authors: Chan Clement Matthew , Martin Darren , Gauthier Emilie , Jensen Paul , Laycock Bronwyn , Pratt Steven
Published: September 2022

Paunch is a fibrous solid residue consisting of partially digested feed from the stomachs of processed cattle. It is the largest untapped solid waste stream from animals at meat processing plants, and potentially a valuable source of fibres for the production of sustainable and potentially higher-value natural biocomposite materials. Paunch was obtained from the waste effluent of a red meat processing plant, and the fibre characteristics of the as-obtained material were studied and benchmarked against wood flour and ground buffel grass, with a view to evaluating the potential of paunch as a fibre for polymer composites. The ground paunch possessed a rough fibrous surface and fibre-like characteristics that were comparable to both wood flour and ground buffel grass, demonstrating their potential for use in composites. Without any pre-treatment or compatibilisation, composites of a representative biopolymer, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and ground paunch were successfully produced for the first time via extrusion, with up to 50 wt% paunch content. Mechanical property analysis showed that, at 30 wt% content, PHBV/ground paunch composites yielded mechanical properties that were comparable to those of composites with ground buffel grass.
Generation of Polyamide 12 Coatings on Stainless Steel Substrates by Directed Energy Deposition with a Thulium-Doped Fiber Laser (DED-LB/P)
Authors: Wittmann Alexander , Hentschel Oliver , Sommereyns Alexander , Schmidt Michael
Published: September 2022

Due to their good material properties (e.g., corrosion and wear resistance, biocompatibility), thermoplastic materials like polyamide 12 (PA12) are interesting for functional coatings on metallic components. To ensure a spatially resolved coating and to shorten the process chain, directed energy deposition of polymer powders by means of a laser beam (DED-LB/P) offers a promising approach. Due to characteristic absorption bands, the use of a thulium fiber laser with a wavelength of 1.94 μm is investigated in a DED-LB/P setup to generate PA12 coatings on stainless steel substrates without the need to add any absorbing additives. The influence of the energy density and powder mass flow was analyzed by infrared thermography. Furthermore, the coatings were characterized by differential scanning calorimetry, laser-scanning-microscopy, optical microscopy and cross-cutting tests. The results in this study demonstrate for the first time the basic feasibility of an absorber-free DED-LB/P process by using a thulium fiber laser. PA12 coatings with a low porosity and good adhesion are achievable. Depending on the application-specific requirements, a trade-off must be made between the density and surface quality of the PA12 coatings. The use of infrared thermography is appropriate for in-situ detection of process instabilities caused by an excessive energy input.
Effect of Carbon Fibres on Electromagnetic-Interference-Shielding Properties of Geopolymer Composites
Authors: Wanasinghe Dimuthu , Aslani Farhad , Ma Guowei
Published: September 2022

Many of the construction materials available are known to cause a drastic level of damage to the environment during their manufacturing stages. Hence, many researchers have attempted to formulate construction materials that are more environmentally friendly. Additionally, the rise in wireless communications in recent decades has seen a rapid increase in electromagnetic pollution and interference, which affects the functionality of sensitive electronic devices. This research is focused on fabricating a more sustainable construction material that could prevent electromagnetic interference for electronic devices housed inside. Carbon fibres of three different lengths were added in four variations to a geopolymer control mix to study their effect on electromagnetic interference shielding. The results showed that the amount of shielding produced by these composites increases with carbon fibre length and quantity. Morphological analyses showed that the interconnectivity of the fibres plays a crucial role in having a high level of shielding. While the flexural strength showed an improvement with the addition of carbon fibre, the compressive strength showed a slight reduction with the increase in carbon fibre length. The optimal level of shielding was produced by the specimen containing 0.7% of 12 mm carbon fibre, which was the maximum amount of fibre of any length used in this study; the optimal level of shielding generated was 43.43 dB within the frequency range of 30 MHz to 1.5 GHz.
Predictive Shapes of Ellipsoid PPDL-PTHF Copolymer Particles Prepared by the Phantom Stretching Technique
Authors: Wischke Christian , Hofmann Dieter
Published: September 2022

Ellipsoidal polymer particles can be prepared from spheres by unidirectional stretching at elevated temperatures, while the particles’ aspect ratios (AR) that result from this phantom stretching methodology are often not precisely predictable. Here, an elastic deformation model was exemplarily evaluated for ~50 µm spherical microparticles from PPDL-PTHF block copolymers. The prolate ellipsoidal particles, obtained by stretching in polyvinyl alcohol phantoms, differed in dimensions at identical relative phantoms elongations up to 150%, depending on the relative polymer composition and their systematically altered mechanical properties. Importantly, the resulting particle shapes within the studied range of AR up to ~4 matched the predictions of the elastic deformation model, which includes information of the elastic moduli of phantom and particle materials. These data suggest that the model may be applicable to predict the conditions needed to precisely prepare ellipsoids of desired AR and may be applicable to various deformable particle materials.
Effect of Single and Synergistic Reinforcement of PVA Fiber and Nano-SiO2 on Workability and Compressive Strength of Geopolymer Composites
Authors: Zhang Peng , Wei Shiyao , Zheng Yuanxun , Wang Fei , Hu Shaowei
Published: September 2022

Geopolymer composites can be used as a proper substitute for ordinary Portland cement, which can reduce carbon dioxide (CO2) emissions and make rational use of industrial waste. In this study, an investigation of the workability and compressive strength of geopolymer composites was carried out through a series of experiments, such as slump flow test, consistency meter test and compressive strength test, to clarify the interaction mechanism among superplasticizer (SP), polyvinyl alcohol (PVA) fiber, Nano-SiO2 (NS) and geopolymer composites, thereby improving the properties of engineered composites. The results showed that with the increase in PVA fiber content, the flowability of geopolymer composites decreased, while the thixotropy increased. With the increase in the NS content, the flowability of geopolymer composites first increased and then decreased, reaching its best at 1.0%, while the thixotropy was the opposite. With the increase in the SP content, the flowability of geopolymer composites increased, while the thixotropy decreased. A significant correlation between thixotropy and flowability of geopolymer composites was found (R2 > 0.85). In addition, the incorporation of single PVA fiber or NS significantly improved the compressive strength of geopolymer composites. Specifically, the compressive strength of geopolymer composites with 0.8% content PVA fiber (60.3 MPa) was 33.4% higher than that without PVA fiber (45.2 MPa), and the compressive strength of geopolymer composites with 1.5% content NS (52.6 MPa) was 16.4% higher than that without NS (45.2 MPa). Considering the synergistic effect, it is found that the compressive strength of geopolymer composites (58.5–63.3 MPa) was significantly higher than that without PVA fiber (45.2–52.6 MPa). However, the flowability and compressive strength of geopolymer composites were only slightly improved compared to that without NS. With the increase in the SP content, the compressive strength of geopolymer composites showed a trend of a slight decrease on the whole. Consequently, the results of this study may be useful for further research in the field of repair and prevention of the delamination of composite structures.
Targeting of Nrf2/PPARγ/NLRP3 Signaling Pathway by Stevia rebudiana Bertoni Extract Provides a Novel Insight into Its Protective Effect against Acute Gouty Arthritis-Induced Synovial Inflammation, Oxidative Stress and Apoptosis in a Rat Model
Authors: Badawy Alaa M. , Taha Medhat , Elazab Sara T. , El-Shenbaby Ibrahim , Alghamdi Bandar A , M Hendawy , Al-Kushi Abdullah G. , Fathy Khaled , Baokbah Tourki A. S. , Ibrahim Mohie Mahmoud
Published: September 2022

Our research work examined the potential protection of Stevia rebaudiana extract against monosodium urate crystals (MSU)-induced acute gouty arthritis in a rat model and its possible underlying mechanism. Forty rats were allocated into four groups (n = 10); a control group; an MSU group, whose rats received 0.1 of MSU single intra-articular injection in the ankle joint on the fifth day of the experiment; an MSU + Stevia group, which received 250 mg/kg/day of Stevia extract orally for seven days and MSU crystals on the fifth day; and an MSU + colchicine group, which was administered colchicine at 0.28 mg/kg daily for seven days and MSU crystals on the fifth day. Pretreatment with Stevia extract mitigated MSU-induced inflammation as evidenced by a decrease of the ankle edema and inflammatory cell infiltration and a significant downregulation of the protein level of NFκB, TNFα, IL-1β, IL6, and IL18 as well as NLRP3 gene expression. Additionally, there was a markedly increased PPARγ gene expression (p < 0.001) compared with the MSU group (p < 0.001) and alleviated oxidative stress via significant upregulating of Nrf2/HO-1. Moreover, the pretreatment attenuated apoptosis by significantly decreasing cytochrome c, Bax, Caspase-3, and by increasing Bcl-2 protein. In conclusion, Stevia extract exhibited strong anti-inflammatory, antioxidant, and antiapoptotic effects against MSU-induced gouty arthritis similar to the standard anti-inflammatory colchicine drugs.
Extraction, Isolation, and Purification of Value-Added Chemicals from Lignocellulosic Biomass
Authors: Chaturvedi Tanmay , Hulkko Laura Sini Sofia , Fredsgaard Malthe , Thomsen Mette Hedegaard
Published: September 2022

This review covers the operating conditions for extracting top value-added chemicals, such as levulinic acid, lactic acid, succinic acid, vanillic acid, 3-hydroxypropionic acid, xylitol, 2,5-furandicarboxylic acid, 5-hydroxymethyl furfural, chitosan, 2,3-butanediol, and xylo-oligosaccharides, from common lignocellulosic biomass. Operating principles of novel extraction methods, beyond pretreatments, such as Soxhlet extraction, ultrasound-assisted extraction, and enzymatic extraction, are also presented and reviewed. Post extraction, high-value biochemicals need to be isolated, which is achieved through a combination of one or more isolation and purification steps. The operating principles, as well as a review of isolation methods, such as membrane filtration and liquid–liquid extraction and purification using preparative chromatography, are also discussed.
Multi-Resource Computing Offload Strategy for Energy Consumption Optimization in Mobile Edge Computing
Authors: Wei Zhe , Yu Xuebin , Zou Lei
Published: September 2022

The energy consumption optimization of edge devices in the mobile edge computing environment is mainly based on computational offload strategy. Most of the current common computing offload strategies only consider a single computing resource and do not comprehensively consider different kinds of computing resources in mobile edge computing environments, which cannot fully reduce the energy consumption of edge devices under the condition of ensuring response time constraints. To solve this problem, a multi-resource computing unloading energy consumption model is proposed in the mobile edge computing environment, and a new fitness calculation method for evaluating the energy consumption of edge devices is designed. Combined with the workflow management system, a multi-resource computing offloading particle swarm optimization task scheduling algorithm for energy consumption optimization in mobile edge computing is proposed. The algorithm can fully reduce the energy consumption of mobile terminals under the condition of considering the response time constraint. Experiments show that, compared with the existing four algorithms, the task scheduling algorithm corresponding to the new strategy has stable convergence and optimal fitness. Under the constraint of user response time, the energy consumption of edge devices in the task scheduling scheme is better than the other four unloading strategies.
Experimental and Numerical Study on Dynamic Characteristics of Droplet Impacting on a Hot Tailings Surface
Authors: Zhao Yan , Wen Liangying , Zhang Yuefeng , Liu Bo , Yang Huan , Deng Qingyu
Published: September 2022

It is quite important to clearly understand the fluid dynamic process of water droplets impacting on a hot extracted titanium tailings surface for the recovery and utilization of tailings. In this research, the finite volume simulations of the droplet impingement were developed by applying the volume-of-fluid method and were validated against experimental results. Droplet-impact experiments were performed by using a high-speed camera. The effects of the Weber number, surface tension value, and contact angle on the spreading factor were quantitatively investigated, and the distributions of pressure, velocity contour, and temperature were analyzed in detail during the impact process. We found that the numerical results of the impact process and spreading factor conform to the experiments. Moreover, the surface tension, contact angle, and Weber number have important consequences for droplet dynamic characteristics. Finally, a new maximum spreading factor model that is governed by Weber numbers is proposed for the extracted titanium tailings surface based on the experimental and numerical results. These findings provide a pathway for controlling dynamic interactions of the droplets impacting on the tailings’ surfaces.
Desilication of Sodium Aluminate Solutions from the Alkaline Leaching of Calcium-Aluminate Slags
Authors: Mwase James Malumbo , Safarian Jafar
Published: September 2022

The desilication of sodium aluminate solutions prior to precipitation of aluminum tri-hydroxides is an essential step in the production of high purity alumina for aluminum production. This study evaluates the desilication of sodium aluminate solutions derived from the leaching of calcium-aluminate slags with sodium carbonate, using CaO, Ca(OH)2, and MgO fine particles. The influence of the amount of CaO used, temperature, and comparisons with Ca(OH)2 and MgO were explored. Laboratory scale test work showed that the optimal conditions for this process were using 6 g/L of CaO at 90 °C for 90 min. This resulted in 92% of the Si being removed with as little as 7% co-precipitation of Al. The other desilicating agents, namely Ca(OH)2 and MgO, also proved effective in removing Si but at slower rates and higher amounts of Al co-precipitated. The characteristics of solid residue obtained after the process indicated that the desilication is via the formation of hydrogarnet, Grossular, and hydrotalcite dominant phases for CaO, Ca(OH)2 and MgO agents, respectively.
A Review of Mo-Si Intermetallic Compounds as Ultrahigh-Temperature Materials
Authors: Jiang Liang , Zheng Bin , Wu Changsong , Li Pengxiang , Xue Tong , Wu Jiandong , Han Fenglan , Chen Yuhong
Published: September 2022

Mo-Si compound-based ultrahigh temperature structural material (UHTM) is a new high-temperature structural material with great potential due to its high melting point, high hardness, and suitable density. As an important material to meet the demands of advanced high-temperature structural applications in the range 1200–1600 °C, in oxidizing and aggressive environments, it has attracted the attention of researchers in aerospace, energy, chemical industry, machinery, mechanical metallurgy, and other fields. This study could provide a reference for the research and application of Mo-Si intermetallic compounds as UHTMs. Based on their crystal structure and properties, we summarized the preparation, limitations, and modification methods of Mo-Si intermetallic compounds and reviewed the research progress in their toughness reinforcement and medium-temperature resistance improvement. Based on the literature review, there has been a certain level of progress in the research on modification of Mo-Si intermetallic compounds worldwide. However, both the high brittleness at room temperature and insufficient strength at high temperature have not been solved yet.
A Framework for Service-Oriented Architecture (SOA)-Based IoT Application Development
Authors: Giao Joao , Nazarenko Artem A. , Luis-Ferreira Fernando , Gonçalves Diogo , Sarraipa Joao
Published: September 2022

In the last decades, the increasing complexity of industrial information technology has led to the emergence of new trends in manufacturing. Factories are using multiple Internet of Things (IoT) platforms to harvest sensor information to improve production. Such a transformation contributes to efficiency growth and reduced production costs. To deal with the heterogeneity of the services within an IoT system, Service-Oriented Architecture (SOA) is referred to in the literature as being advantageous for the design and development of software to support IoT-based production processes. The aim of SOA-based design is to provide the leverage to use and reuse loosely coupled IoT services at the middleware layer to minimise system integration problems. We propose a system architecture that follows the SOA architectural pattern and enables developers and business process designers to dynamically add, query or use instances of existing modular software in the IoT context. Furthermore, an analysis of utilization of modular software that presents some challenges and limitations of this approach is also in the scope of this work.
The Roadway Layout and Control Technology of Pillar-Free Mining of Soft Coal Seams in High Gassy Mines
Authors: Ma Qi , Zhang Yidong , Li Zexin , Song Guangyuan , Zheng Yu
Published: September 2022

High gassy mines have low recovery rates of coal resources in the stoping of high-seam coal resources in addition to difficulties in the gas control of the working face and gas accumulation in the goaf and upper corner. Pillar-free gob-side entry-retaining technology was combined with the high-pumping roadway for gas control in the goaf based on the W1319 working face in the Gaohe Coal Mine. Theoretical analysis and numerical simulation were used to derive the width of the reserved coal pillar in the high-gas pillar-free working face and the horizon of the high-pumping roadway. The location of the high-pumping roadway was determined in combination with the on-site investigation. Numerical simulations were used to compare the plastic failures and deformations of surrounding rocks in the cases of non-roof-cutting, roof cutting, and reinforced roof cutting. It could solve large and uncontrolled surrounding rock deformations of the gob-side entry-retaining technology and return airway in the stoping of the working face. The plasticizing zone and surrounding rock deformation of the gob-side entry-retaining technology were weakened in the case of roof cutting. However, the surrounding-rock failure of the return airway was not significantly weakened. The plastic failure of surrounding rocks in the gob-side entry-retaining technology and return airway weakened after the roof-cutting pressure relief and grouting reinforcement, and deformations reduced greatly. The surrounding-rock control measures of roof-cutting pressure relief and grouting reinforcement were determined. The gob-side entry-retaining technology had small surrounding-rock deformations in the advance of the working face, which verified the reliability of numerical simulations and the feasibility of the technical measures of roof-cutting pressure relief and grouting reinforcement. Meanwhile, the high-pumping roadway had a good drainage effect to avoid gas accumulation in the goaf, which provides a reference for the mining of high-seam coal resources.
Green Extraction Process of Food Grade C-phycocyanin: Biological Effects and Metabolic Study in Mice
Authors: Fratelli Camilly , Bürck Monize , Silva-Neto Artur Francisco , Oyama Lila Missae , De Rosso Veridiana Vera , Braga Anna Rafaela Cavalcante
Published: September 2022

This study aimed to evaluate different parameters in the green process of organic Spirulina biomass (SB) C-phycocyanin (C-PC) extraction to understand the impact on weight and oral glucose tolerance of C-PC extract in Swiss mice fed with a high-fat diet (HFD). The proximate composition and antioxidant activity were analyzed in Spirulina by-products: SB, C-PC, and Remaining biomass (RB). The protein content on a dry basis was 52.05% in SB and 61.16% in RB and 118.97 μg/g in C-PC. The antioxidant activity was equal for SB and C-PC but higher than RB. However, RB can be considered a promising ingredient, promoting the sustainable use of the whole SB. Swiss mice were distributed in five groups: control diet (CD), HFD, HFD plus Spirulina biomass (HFDS), HFD plus C-PC (HFDC), and HFD plus remaining biomass (HFDR). HFDS increased the delta weight of the animals and showed glucose intolerance compared to the CD and HFDC groups. The results demonstrated that the supplementation of 500 mg/kg of body weight of SB in the HFDS group did not show antiobesogenic potential with an HFD, but it is essential to conduct further studies to bring other interesting responses regarding C-PC biological in vivo effects.
Research and Performance Evaluation on Selective Absorption of H2S from Gas Mixtures by Using Secondary Alkanolamines
Authors: Xue Jingwen , Yang Chaoyue , Fu Jingqiang , He Jinlong , Li Jinjin
Published: September 2022

Exploring new solvents for efficient acid gas removal is one of the most attractive topics in industrial gas purification. Herein, using 2-tertiarybutylamino-2-ethoxyethanol as an absorbent in a packed column at atmospheric pressure was examined for selective absorption of H2S from mixed gas streams. In the present work, the acid gas load, H2S absorption selectivity, acid gas removal ratio, amine solution regeneration performance, and corrosion performance were investigated through evaluating experiments absorbing H2S and CO2 by using methyldiethanolamine and 2-tertiarybutylamino-2-ethoxyethanol. The experimental results illustrate that the H2S absorption selective factors were 3.88 and 15.81 by using 40% methyldiethanolamine and 40% 2-tertiarybutylamino-2-ethoxyethanol at 40 °C, respectively, showing that 2-tertiarybutylamino-2-ethoxyethanol is an efficient solvent for selective H2S removal, even better than methyldiethanolamine. Based on the consideration of cost, we added 5% TBEE to 35% MDEA to form a blended aqueous solvent. To our satisfaction, the blended amine solvent obtained a 99.79% H2S removal rate and a 22.68% CO2 co-absorption rate, while using the methyldiethanolamine alone achieved a 98.33% H2S removal rate and a 23.52% CO2 co-absorption rate; the blended solvent showed better H2S absorption efficiency and selectivity. Taken together, this work provides valuable information for a promising alkanolamine for acid gas removal, and the preliminary study has found that the aqueous blend of methyldiethanolamine and 2-tertiarybutylamino-2-ethoxyethanol is an efficient solvent for selective H2S removal, which not only extends the application field for sterically hindered amines, but also opens up new opportunities in blended solvent design.
Novel Process for Butyl Acetate Production via Membrane Reactor: A Comparative Study with the Conventional and Reactive Distillation Processes
Authors: Al-Rabiah Abdulrahman A. , Alqahtani Abdullah E. , Al Darwish Rayan K. , Bin Naqyah Abdulaziz S.
Published: September 2022

Butyl acetate (BuAc) is widely used as a solvent in many applications, mainly in the food and pharmaceutical industries. The conventional process for BuAc production is both capital and energy intensive. The purification process involves the separation of BuAc from the azeotropic mixture of water and n-butanol, which is difficult to accomplish using a simple distillation unit. In this study, a membrane reactor (MR) for BuAc production via the esterification of n-butanol was investigated. The MR using the Amberlyst-15 catalyst was modeled and validated with previously reported experimental data, and a good agreement was achieved. The ultimate conversion of n-butanol using the MR was 92.0%, compared to 69.8% for the conventional reactor. This study is the first to propose an intensified MR-based process for butyl acetate production. The MR-based process was developed and rigorously simulated using Aspen Plus for an annual plant capacity of 92,500 metric tons of BuAc. The MR-based process is environmentally friendly regarding CO2 emissions, with a reduction of 80% compared to the conventional process. The economic analysis of the MR-based process shows a payback period of 2.7 years and a return on investment (ROI) of 23.1%. The MR-based process for BuAc production is a promising technology that provides similar key benefits as compared to the reactive distillation (RD) process.
Obtention of New Edible Biofilms from Water Kefir Grains in Comparison with Conventional Biofilms from Taro (Colocasia esculenta) and Cassava (Manihot esculenta) Starch
Authors: Linares-Bravo Paul , Cabo-Araoz Samantha D. , Luna-Solano Guadalupe , Urrea-Garcia Galo R. , Cantú-Lozano Denis
Published: September 2022

Microorganism biomass is a sustainable and innovative source of biopolymers, such as proteins and polysaccharides, that is suitable for the development of biodegradable films. The aim of this research was to evaluate the synthesis, morphology, rheology, and morphological and mechanical properties on the production of edible biofilms based on water kefir grains, and compare them with edible films based on thermoplastic compounds from starch (TPS) obtained from taro (Colocasia esculenta) and cassava (Manihot esculenta). Edible biofilms were prepared in solution with 30% wt/wt glycerol relative to starch mass and kefir grain biofilms using the casting method. A stationary rheological analysis was performed on the film-forming suspensions of kefir, taro starch, and cassava starch. Once the films were obtained, a physicochemical and morphological characterization was carried out. Results of the characterization showed the following main aspects: The results indicated an increase in biomass production using muscovado and pineapple peel. The film-forming suspensions had a dilating behavior; however, the results obtained not only show the viscoelastic behavior but also the elastic limit (σ0), which varied from 0.077 to 0.059 Pa for suspensions of water kefir grains and from 0.077 to 0.072 Pa for starch suspensions. These elastic limit variations can be defined as the minimum shear stress required to start the flow, and all these rheological data were adjusted to the Herschel–Bulkley model; the morphological and mechanical characterization of the films obtained showed homogeneous surfaces with transparency and without cracks; regarding the water activity, values lower than 6 were obtained, which indicates that there will be no growth of any microorganism, and the hardness data showed differences between those obtained from kefir and taro and cassava starch. The similar results of the rheological characterization in the formation of the kefir biofilm and the conventional edible starch films, in addition to the similar results in the water activity below 6 and the hardness, points to an attractive alternative capable of replacing the conventional materials with a mass production of biofilms of probiotic microorganisms. The results also revealed that water kefir grains biomass is a viable and innovative source of biodegradable materials, and these grains can be an alternative to conventional established starch materials.
Evaluating the Potential of Glycyrrhiza uralensis (Licorice) in Treating Alcoholic Liver Injury: A Network Pharmacology and Molecular Docking Analysis Approach
Authors: Zhu Lichun , Xie Shuangquan , Geng Zhihua , Yang Xuhai , Zhang Qian
Published: September 2022

Glycyrrhiza uralensis is used to treat alcoholic liver injury in China; however, its pharmacological mechanism remains to be clarified. Here, the potential of G. uralensis for the treatment of alcoholic liver injury was explored using a network pharmacology and molecular docking approach. The effective components and targets of G. uralensis were searched in a traditional Chinese medicine systems pharmacology database and analysis platform. Disease targets were obtained using the GeneCards and OMIM databases. The target genes of G. uralensis and alcoholic liver injury were compared to obtain common target genes. Symbol conversion was carried out using the Uniport database, and the composition–target network of G. uralensis and alcoholic liver injury was prepared. A protein–protein interaction network was constructed. Gene Ontology functional enrichment and the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed. AutoDock was used for the molecular docking of core compounds and key targets. One hundred and ninety-three common target genes of G. uralensis and alcoholic liver injury were screened. We identified targets of five active components of G. uralensis, namely, formononetin, 3′-methoxyglabridin, glypallichalcone, 1-methoxyphaseollidin, and glabridin. The main targets identified via the protein–protein interaction network analysis were JUN, MAPK3, STAT3, AKT1, and MAPK1. The biological processes associated with xenobiotic stimulus and lipopolysaccharide metabolism were involved. These biological processes were common between Glycyrrhiza treatment and liver injury. They mainly involved lipid and atherosclerosis, chemical carcinogenic gene-receptor activation, and Kaposi sarcoma-associated herpesvirus. Shinpterocarpin and 7-methoxy-2-methyl-isoflavone had good docking effects with MAPK3, and their binding energies were less than −5 kcal/mol. Based on the network pharmacology and molecular docking analyses, the chemical compositions, potential targets, and pathways involved in G. uralensis treatment of alcoholic liver injury were successfully predicted. This study lays a foundation for the selection of drugs to treat alcoholic liver injury.
Antioxidative Properties and Phenolic Profile of the Core, Pulp and Peel of Commercialized Kiwifruit by LC-ESI-QTOF-MS/MS
Authors: Liu Zifan , Shi Linghong , Qi Yunyang , Barrow Colin J. , Dunshea Frank R. , Suleria Hafiz A. R.
Published: September 2022

The kiwifruit is cultivated globally due to its diversity of phytochemicals, especially phenolic compounds, which have antioxidant, anti-inflammatory and anti-cancer medical effects. However, only the pulp of the kiwifruit is consumed, while the peels and cores—which are also rich in phytochemicals—are usually wasted. Meanwhile, detailed information on the comparison among the three parts is still limited. In this study, the antioxidant potentials in the core, pulp, and peel of the three most commercialized kiwifruit cultivars (Australian-grown Hayward kiwifruit, New Zealand-grown Zesy002 kiwifruit, and New Zealand-grown organic Hayward kiwifruit) were selected. Their antioxidant capacities were tested, and their phenolic profiles were identified and characterized by liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS/MS). The antioxidant results showed that the peel of New Zealand-grown organic Hayward kiwifruit contained the highest total phenolic content (9.65 mg gallic acid equivalent (GAE) mg/g) and total antioxidant capacity (4.43 mg ascorbic acid equivalent (AAE) mg/g), respectively. In addition, the antioxidant capacity of the peel is generally higher than that of the pulp and cores in all species, especially ABTS (2,2-Azino-bis-3ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging ability), ranging from 13.25 mg AAE/g to 18.31 mg AAE/g. The LC-ESI-QTOF-MS/MS tentatively identified the phenolic compounds present in the three kiwifruit species, including 118 unique compounds in kiwifruit peel, 12 unique compounds in the kiwifruit cores, and three unique compounds in kiwifruit pulp. The comprehensive characterization of the phenolics in the kiwifruits’ parts indicates the importance of their waste part as a promising source of phenolics with antioxidant properties. Therefore, this study can guide the industry with meaningful information on kiwifruit waste, and can provide it with the utilization of food and pharmacological aspects.