bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Non-Coding RNAs on Chemotherapeutic Resistance in Oral Cancer
Authors: Yamaguchi Karen , Yamamoto Tomofumi , Chikuda Junichiro , Shirota Tatsuo , Yamamoto Yusuke
Published: February 2022

Drug resistance in oral cancer is one of the major problems in oral cancer therapy because therapeutic failure directly results in tumor recurrence and eventually in metastasis. Accumulating evidence has demonstrated the involvement of non-coding RNAs (ncRNAs), such as microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), in processes related to the development of drug resistance. A number of studies have shown that ncRNAs modulate gene expression at the transcriptional or translational level and regulate biological processes, such as epithelial-to-mesenchymal transition, apoptosis, DNA repair and drug efflux, which are tightly associated with drug resistance acquisition in many types of cancer. Interestingly, these ncRNAs are commonly detected in extracellular vesicles (EVs) and are known to be delivered into surrounding cells. This intercellular communication via EVs is currently considered to be important for acquired drug resistance. Here, we review the recent advances in the study of drug resistance in oral cancer by mainly focusing on the function of ncRNAs, since an increasing number of studies have suggested that ncRNAs could be therapeutic targets as well as biomarkers for cancer diagnosis.
An Integrative Approach to Characterize the Early Phases of Dimethylhydrazine-Induced Colorectal Carcinogenesis in the Rat
Authors: Silva-Reis Rita , Castro-Ribeiro Catarina , Gonçalves Mariana , Ferreira Tiago , Pires Maria João , Iglesias-Aguirre Carlos E. , Cortés-Martín Adrián , Selma María V. , Espín Juan Carlos , Nascimento-Gonçalves Elisabete , Moreira-Pais Alexandra , Neuparth Maria J. , Peixoto Francisco , Rosa Eduardo , Gama Adelina , Ferreira Rita , Oliveira Paula A. , Faustino-Rocha Ana I.
Published: February 2022

This study aimed to characterize an animal model of colorectal cancer (CRC) in the early stages of disease development. Twenty-nine male Wistar rats were divided into two control groups (CTRL1 and CTRL2), receiving EDTA–saline injections and two induced groups (CRC1 and CRC2), receiving 1,2-dimethylhydrazine (DMH) injections for seven consecutive weeks. CRC1 and CTRL1 were euthanized at the 11th week, while CRC2 and CTRL2 were euthanized at the 17th week. DMH treatment decreased microhematocrit values and IL-6, ghrelin, and myostatin serum levels. Histopathological analysis of intestinal sections showed that DMH-treated rats were characterized by moderate to severe epithelial dysplasia. An adenoma was observed in one animal (CRC2 group), and the presence of inflammatory infiltrate at the intestinal level was primarily observed in DMH-treated animals. DMH also induced Ki-67 immunoexpression. The gut microbiota analysis showed a higher abundance of Firmicutes, Clostridia, Clostridiales, Peptostreptococcaceae, Blautia, Romboutsia, and Clostridium sensu stricto in CRC than CTRL rats, whereas Prevotellaceae, Prevotella, Akkermansia, and Lactobacillus levels were more prevalent in CTRL animals. Our results suggest that this model could be helpful to investigate chemoprevention in the early stages of CRC.
The Combination of Galanin (1–15) and Escitalopram in Rats Suggests a New Strategy for Alcohol Use Disorder Comorbidity with Depression
Authors: Cantero-García Noelia , Flores-Burgess Antonio , Ladrón de Guevara-Miranda David , Serrano Antonia , García-Durán Laura , Puigcerver Araceli , Fuxe Kjell , Narváez José Ángel , Santín Luis Javier , Díaz-Cabiale Zaida , Millón Carmelo
Published: February 2022

Alcohol use disorder (AUD) is highly prevalent, and over 50% of AUD patients also suffer major depressive disorders. Selective 5-HT reuptake inhibitors (SSRIs) can reduce rodent ethanol drinking but exert modest clinical efficacy in alcoholic individuals. Finding new pharmacological strategies that could modulate alcohol consumption and depression is necessary. We have analyzed the effect of Galanin (1–15) [GAL(1–15)] on escitalopram (ESC)-mediated effect in alcohol consumption using the alcohol self-administration test, the nuclei involved in the effect, and whether GAL(1–15) + ESC modulated the response in despair or anxiety tests in animals under chronic alcohol intake. GAL(1–15) + ESC combination substantially reduced alcohol intake in the alcohol self-administration test and, moreover, enhanced the reduction of reward capacity of ESC on different reinforcers such as sucrose or saccharine. GAL(1–15) + ESC coadministration significantly decreases the number of C-Fos-IR TH cell bodies in the VTA, and PCA analysis suggests that one functional network, including VTA, RMTg and DR, is involved in these effects. Significantly in rats with chronic alcohol consumption, GAL(1–15) reversed adverse ESC-mediated effects in the depression-related behavioural test and forced swimming test. The results open up the possibility of using GAL(1–15) in combination with the SSRI Escitalopram as a novel strategy in AUD comorbidity with depression.
Selective Detection of Legionella pneumophila Serogroup 1 and 5 with a Digital Photocorrosion Biosensor Using Antimicrobial Peptide-Antibody Sandwich Strategy
Authors: Islam M. Amirul , Hassen Walid M. , Ishika Ishika , Tayabali Azam F. , Dubowski Jan J.
Published: February 2022

Rapid detection of Legionella pneumophila (L. pneumophila) is important for monitoring the presence of these bacteria in water sources and preventing the transmission of the Legionnaires’ disease. We report improved biosensing of L. pneumophila with a digital photocorrosion (DIP) biosensor functionalized with an innovative structure of cysteine-modified warnericin antimicrobial peptides for capturing bacteria that are subsequently decorated with anti-L. pneumophila polyclonal antibodies (pAbs). The application of peptides for the operation of a biosensing device was enabled by the higher bacterial-capture efficiency of peptides compared to other traditional ligands, such as those based on antibodies or aptamers. At the same time, the significantly stronger affinity of pAbs decorating the L. pneumophila serogroup-1 (SG-1) compared to serogroup-5 (SG-5) allowed for the selective detection of L. pneumophila SG-1 at 50 CFU/mL. The results suggest that the attractive sensitivity of the investigated sandwich method is related to the flow of an extra electric charge between the pAb and a charge-sensing DIP biosensor. The method has the potential to offer highly specific and sensitive detection of L. pneumophila as well as other pathogenic bacteria and viruses.
A Pilot Feasibility Study of Reconnecting to Internal Sensations and Experiences (RISE), a Mindfulness-Informed Intervention to Reduce Interoceptive Dysfunction and Suicidal Ideation, among University Students in India
Authors: Smith April R. , Kinkel-Ram Shruti , Grunwald William , George Tony Sam , Raval Vaishali
Published: February 2022

Although 20% of the world’s suicides occur in India, suicide prevention efforts in India are lagging (Vijayakumar et al., 2021). Identification of risk factors for suicide in India, as well as the development of accessible interventions to treat these risk factors, could help reduce suicide in India. Interoceptive dysfunction—or an inability to recognize internal sensations in the body—has emerged as a robust correlate of suicidality among studies conducted in the United States. Additionally, a mindfulness-informed intervention designed to reduce interoceptive dysfunction, and thereby suicidality, has yielded promising initial effects in pilot testing (Smith et al., 2021). The current studies sought to replicate these findings in an Indian context. Study 1 (n = 276) found that specific aspects of interoceptive dysfunction were related to current, past, and future likelihood of suicidal ideation. Study 2 (n = 40) was a small, uncontrolled pre-post online pilot of the intervention, Reconnecting to Internal Sensations and Experiences (RISE). The intervention was rated as highly acceptable and demonstrated good retention. Additionally, the intervention was associated with improvements in certain aspects of interoceptive dysfunction and reductions in suicidal ideation and eating pathology. These preliminary results suggest further testing of the intervention among Indian samples is warranted.
Domain-General Cognitive Skills in Children with Mathematical Difficulties and Dyscalculia: A Systematic Review of the Literature
Authors: Agostini Francesca , Zoccolotti Pierluigi , Casagrande Maria
Published: February 2022

Mathematical performance implies a series of numerical and mathematical skills (both innate and derived from formal training) as well as certain general cognitive abilities that, if inadequate, can have a cascading effect on mathematics learning. These latter skills were the focus of the present systematic review. Method: The reviewing process was conducted according to the PRISMA statement. We included 46 studies comparing school-aged children’s performance with and without math difficulties in the following cognitive domains: processing speed, phonological awareness, short- and long-term memory, executive functions, and attention. Results: The results showed that some general cognitive domains were compromised in children with mathematical difficulties (i.e., executive functions, attention, and processing speed). Conclusions: These cognitive functions should be evaluated during the diagnostic process in order to better understand the child’s profile and propose individually tailored interventions. However, further studies should investigate the role of skills that have been poorly investigated to date (e.g., long-term memory and phonological awareness).
Next Steps: Applying a Trauma-Informed Model to Create an Anti-Racist Organizational Culture
Authors: Esaki Nina , Reddy Maxine , Bishop Cameron T.
Published: February 2022

Although there has been a significant increase in the delivery of evidence-supported, trauma-informed care over the past few years, there has been less discussion around the consideration of the broader cultural, political, and societal factors that contextualize client trauma and that also need to be recognized and understood to promote healing and prevent future trauma. In support of sharing some best practices and lessons learned, this article provides a case study of one agency that has used the Sanctuary Model®, an evidence-supported, trauma-informed organizational change model, to introduce the practice of cultural humility with staff as a facilitator of improved service delivery for clients from culturally marginalized communities. The model supports these endeavors through the adherence to the seven commitments, a set of organizational values for creating a trauma-informed community, allowing for all voices to be heard and considered and providing opportunities to begin the repair of previous experiences of inequity and suppression. Through the board of directors, leadership, and staff, the organization transformed its culture into one that truly supports and embraces diversity, equity, and inclusion in its operation for the benefit of both staff and clients alike.
Administering Docetaxel for Metastatic Hormone-Sensitive Prostate Cancer 1–6 Days Compared to More Than 14 Days after the Start of LHRH Agonist Is Associated with Better Clinical Outcomes Due to Androgen Flare
Authors: Nasser Nicola J. , Sun Kai , Scanlon Karen M. , Mishra Mark V. , Molitoris Jason K.
Published: February 2022

Docetaxel, when given at the beginning of androgen deprivation therapy (ADT) for patients with metastatic hormone-sensitive prostate cancer (MHSPC), results in significantly longer overall survival than ADT alone. We aimed to investigate if the delivery of the first dose of docetaxel during the testosterone flare associated with LHRH initiation results in better clinical outcomes, as testosterone induces mitosis of prostate cancer cells, and docetaxel specifically targets cells in mitosis. We analyzed data from the CHAARTED trial which randomized MHSPC patients to ADT alone or ADT plus docetaxel. We included only patients treated with LHRH agonist and docetaxel (n = 379). The only cutoff that resulted in differences in treatment outcomes was between patients who started docetaxel 1–6 days (n = 18) compared to more than 14 days from LHRH initiation (n = 297). Actuarial median overall survival was 72 versus 57 months (p = 0.2); progression-free survival was 49 versus 17 months (p = 0.06), and freedom from castrate-resistant prostate cancer was 51 versus 18 months (p = 0.04) for patients who started docetaxel 1–6 days compared to more than 14 days from LHRH initiation, respectively. Administering docetaxel 1–6 days from the initiation of LHRH agonist for patients with MHSPC could be associated with improved clinical outcomes.
Novel Gene Signatures as Prognostic Biomarkers for Predicting the Recurrence of Hepatocellular Carcinoma
Authors: Son Ju A , Ahn Hye Ri , You Donglim , Baek Geum Ok , Yoon Moon Gyong , Yoon Jung Hwan , Cho Hyo Jung , Kim Soon Sun , Nam Suk Woo , Eun Jung Woo , Cheong Jae Youn
Published: February 2022

Hepatocellular carcinoma (HCC) has a high rate of cancer recurrence (up to 70%) in patients who undergo surgical resection. We investigated prognostic gene signatures for predicting HCC recurrence using in silico gene expression analysis. Recurrence-associated gene candidates were chosen by a comparative analysis of gene expression profiles from two independent whole-transcriptome datasets in patients with HCC who underwent surgical resection. Five promising candidate genes, CETN2, HMGA1, MPZL1, RACGAP1, and SNRPB were identified, and the expression of these genes was evaluated using quantitative reverse transcription PCR in the validation set (n = 57). The genes CETN2, HMGA1, RACGAP1, and SNRPB, but not MPZL1, were upregulated in patients with recurrent HCC. In addition, the combination of HMGA1 and MPZL1 demonstrated the best area under the curve (0.807, 95% confidence interval [CI] = 0.681–0.899) for predicting HCC recurrence. In terms of clinicopathological correlation, CETN2, MPZL1, RACGAP1, and SNRPB were upregulated in patients with microvascular invasion, and the expression of MPZL1 and SNRPB was increased in proportion to the Edmonson tumor differentiation grade. Additionally, overexpression of CETN2, HMGA1, and RACGAP1 correlated with poor overall survival (OS) and disease-free survival (DFS) in the validation set. Finally, Cox regression analysis showed that the expression of serum alpha-fetoprotein and RACGAP1 significantly affected OS, whereas platelet count, microvascular invasion, and HMGA1 expression significantly affected DFS. In conclusion, HMGA1 and RACGAP1 may be potential prognostic biomarkers for predicting the recurrence of HCC after surgical resection.
The Advances in Glioblastoma On-a-Chip for Therapy Approaches
Authors: Alves Arielly H. , Nucci Mariana P. , Mamani Javier B. , Valle Nicole M. E. , Ribeiro Eduarda F. , Rego Gabriel N. A. , Oliveira Fernando A. , Theinel Matheus H. , Santos Ricardo S. , Gamarra Lionel F.
Published: February 2022

This systematic review aimed to verify the use of microfluidic devices in the process of implementing and evaluating the effectiveness of therapeutic approaches in glioblastoma on-a-chip, providing a broad view of advances to date in the use of this technology and their perspectives. We searched studies with the variations of the keywords “Glioblastoma”, “microfluidic devices”, “organ-on-a-chip” and “therapy” of the last ten years in PubMed and Scopus databases. Of 446 articles identified, only 22 articles were selected for analysis according to the inclusion and exclusion criteria. The microfluidic devices were mainly produced by soft lithography technology, using the PDMS material (72%). In the microenvironment, the main extracellular matrix used was collagen type I. Most studies used U87-MG glioblastoma cells from humans and 31.8% were co-cultivated with HUVEC, hCMEC/D3, and astrocytes. Chemotherapy was the majority of therapeutic approaches, assessing mainly the cellular viability and proliferation. Furthermore, some alternative therapies were reported in a few studies (22.6%). This study identified a diversity of glioblastoma on-a-chip to assess therapeutic approaches, often using intermediate levels of complexity. The most advanced level implemented the intersection between different biological systems (liver–brain or intestine–liver–brain), BBB model, allowing in vitro studies with greater human genetic similarity, reproducibility, and low cost, in a highly customizable platform.
Over-Reduced State of Mitochondria as a Trigger of “β-Oxidation Shuttle” in Cancer Cells
Authors: Zhelev Zhivko , Sumiyoshi Akira , Aoki Ichio , Lazarova Dessislava , Vlaykova Tatyana , Higashi Tatsuya , Bakalova Rumiana
Published: February 2022

A considerable amount of data have accumulated in the last decade on the pronounced mitochondrial fatty acid oxidation (mFAO) in many types of cancer cells. As a result, mFAO was found to coexist with abnormally activated fatty acid synthesis (FAS) and the mevalonate pathway. Recent studies have demonstrated that overactivated mitochondrial β-oxidation may aggravate the impaired mitochondrial redox state and vice versa. Furthermore, the impaired redox state of cancerous mitochondria can ensure the continuous operation of β-oxidation by disconnecting it from the Krebs cycle and connecting it to the citrate–malate shuttle. This could create a new metabolic state/pathway in cancer cells, which we have called the “β-oxidation-citrate–malate shuttle”, or “β-oxidation shuttle” for short, which forces them to proliferate. The calculation of the phosphate/oxygen ratio indicates that it is inefficient as an energy source and must consume significantly more oxygen per mole of ATP produced when combined with acetyl-CoA consuming pathways, such as the FAS and mevalonate pathways. The “β-oxidation shuttle” is an unconventional mFAO, a separate metabolic pathway that has not yet been explored as a source of energy, as well as a source of cataplerosis, leading to biomass accumulation, accelerated oxygen consumption, and, ultimately, a source of proliferation. The role of the “β-oxidation shuttle” and its contribution to redox-altered cancer metabolism provides a new direction for the development of future anticancer strategies. This may represent the metabolic “secret” of cancer underlying hypoxia and genomic instability.
The Role of microRNAs in Cancer: Functions, Biomarkers and Therapeutics
Authors: Tucci Paola
Published: February 2022

MicroRNAs (miRs) are small non-coding RNAs acting as post-transcriptional regulators of gene expression with important roles in almost all biological pathways, including development, differentiation, cell cycle, proliferation, and apoptosis [...]
Thermochemical Conversion of Untreated and Pretreated Biomass for Efficient Production of Levoglucosenone and 5-Chloromethylfurfural in the Presence of an Acid Catalyst
Authors: Kundu Chandan , Biswas Saheli , Kibria Mahmud Arman , Bhattacharya Sankar
Published: February 2022

Levoglucosenone (LGO) and 5-chloromethyl furfural (5-CMF) are two bio-based platform chemicals with applications in medicines, green solvents, fuels, and the polymer industry. This study demonstrates the one-step thermochemical conversion of raw and pretreated (delignified) biomass to highly-valuable two platform chemicals in a fluidized bed reactor. Hydrochloric acid gas is utilized to convert biomass thermochemically. The addition of hydrochloric acid gas facilitates the formation of LGO and CMF. Acid gas reacts with biomass to form 5-CMF, which acts as a catalyst to increase the concentration of LGO in the resulting bio-oil. The presence of higher cellulose content in delignified biomass significantly boosts the synthesis of both platform chemicals (LGO and CMF). GC-MS analysis was used to determine the chemical composition of bio-oil produced from thermal and thermochemical conversion of biomass. At 350 °C, the maximum concentration of LGO (27.70 mg/mL of bio-oil) was achieved, whereas at 400 °C, the highest concentration of CMF (19.24 mg/mL of bio-oil) was obtained from hardwood-delignified biomass. The findings suggest that 350 °C is the optimal temperature for producing LGO and 400 °C is optimal for producing CMF from delignified biomass. The secondary cracking process is accelerated by temperatures over 400 °C, resulting in a low concentration of the target platform chemicals. This work reveals the simultaneous generation of LGO and CMF, two high-value commercially relevant biobased compounds.
Metal-Supported Biochar Catalysts for Sustainable Biorefinery, Electrocatalysis, and Energy Storage Applications: A Review
Authors: Ramos Rubén , Abdelkader-Fernández Víctor K. , Matos Renata , Peixoto Andreia F. , Fernandes Diana M.
Published: February 2022

Biochar (BCH) is a carbon-based bio-material produced from thermochemical conversion of biomass. Several activation or functionalization methods are usually used to improve physicochemical and functional properties of BCHs. In the context of green and sustainable future development, activated and functionalized biochars with abundant surface functional groups and large surface area can act as effective catalysts or catalyst supports for chemical transformation of a range of bioproducts in biorefineries. Above the well-known BCH applications, their use as adsorbents to remove pollutants are the mostly discussed, although their potential as catalysts or catalyst supports for advanced (electro)catalytic processes has not been comprehensively explored. In this review, the production/activation/functionalization of metal-supported biochar (M-BCH) are scrutinized, giving special emphasis to the metal-functionalized biochar-based (electro)catalysts as promising catalysts for bioenergy and bioproducts production. Their performance in the fields of biorefinery processes, and energy storage and conversion as electrode materials for oxygen and hydrogen evolutions, oxygen reduction, and supercapacitors, are also reviewed and discussed.
Muscle Sonography in Inclusion Body Myositis: A Systematic Review and Meta-Analysis of 944 Measurements
Authors: Abdelnaby Ramy , Mohamed Khaled Ashraf , Elgenidy Anas , Sonbol Yousef Tarek , Bedewy Mahmoud Mostafa , Aboutaleb Aya Moustafa , Ebrahim Mohamed Ayman , Maallem Imene , Dardeer Khaled Tarek , Heikal Hamed Amr , Gawish Hazem Maher , Zschüntzsch Jana
Published: February 2022

Inclusion body myositis (IBM) is a slowly progressive muscle weakness of distal and proximal muscles, which is diagnosed by clinical and histopathological criteria. Imaging biomarkers are inconsistently used and do not follow international standardized criteria. We conducted a systematic review and meta-analysis to investigate the diagnostic value of muscle ultrasound (US) in IBM compared to healthy controls. A systematic search of PubMed/MEDLINE, Scopus and Web of Science was performed. Articles reporting the use of muscle ultrasound in IBM, and published in peer-reviewed journals until 11 September 2021, were included in our study. Seven studies were included, with a total of 108 IBM and 171 healthy controls. Echogenicity between IBM and healthy controls, which was assessed by three studies, demonstrated a significant mean difference in the flexor digitorum profundus (FDP) muscle, which had a grey scale value (GSV) of 36.55 (95% CI, 28.65–44.45, p < 0.001), and in the gastrocnemius (GC), which had a GSV of 27.90 (95% CI 16.32–39.48, p < 0.001). Muscle thickness in the FDP showed no significant difference between the groups. The pooled sensitivity and specificity of US in the differentiation between IBM and the controls were 82% and 98%, respectively, and the area under the curve was 0.612. IBM is a rare disease, which is reflected in the low numbers of patients included in each of the studies and thus there was high heterogeneity in the results. Nevertheless, the selected studies conclusively demonstrated significant differences in echogenicity of the FDP and GC in IBM, compared to controls. Further high-quality studies, using standardized operating procedures, are needed to implement muscle ultrasound in the diagnostic criteria.
Biomaterials and Cell-Based Regenerative Therapies for Intervertebral Disc Degeneration with a Focus on Biological and Biomechanical Functional Repair: Targeting Treatments for Disc Herniation
Authors: Yamada Katsuhisa , Iwasaki Norimasa , Sudo Hideki
Published: February 2022

Intervertebral disc (IVD) degeneration is a common cause of low back pain and most spinal disorders. As IVD degeneration is a major obstacle to the healthy life of so many individuals, it is a major issue that needs to be overcome. Currently, there is no clinical treatment for the regeneration of degenerated IVDs. However, recent advances in regenerative medicine and tissue engineering suggest the potential of cell-based and/or biomaterial-based IVD regeneration therapies. These treatments may be indicated for patients with IVDs in the intermediate degenerative stage, a point where the number of viable cells decreases, and the structural integrity of the disc begins to collapse. However, there are many biological, biomechanical, and clinical challenges that must be overcome before the clinical application of these IVD regeneration therapies can be realized. This review summarizes the basic research and clinical trials literature on cell-based and biomaterial-based IVD regenerative therapies and outlines the important role of these strategies in regenerative treatment for IVD degenerative diseases, especially disc herniation.
MSK-Mediated Phosphorylation of Histone H3 Ser28 Couples MAPK Signalling with Early Gene Induction and Cardiac Hypertrophy
Authors: Robinson Emma L. , Drawnel Faye M. , Mehdi Saher , Archer Caroline R. , Liu Wei , Okkenhaug Hanneke , Alkass Kanar , Aronsen Jan Magnus , Nagaraju Chandan K. , Sjaastad Ivar , Sipido Karin R. , Bergmann Olaf , Arthur J. Simon C. , Wang Xin , Roderick H. Llewelyn
Published: February 2022

Heart failure is a leading cause of death that develops subsequent to deleterious hypertrophic cardiac remodelling. MAPK pathways play a key role in coordinating the induction of gene expression during hypertrophy. Induction of the immediate early gene (IEG) response including activator protein 1 (AP-1) complex factors is a necessary and early event in this process. How MAPK and IEG expression are coupled during cardiac hypertrophy is not resolved. Here, in vitro, in rodent models and in human samples, we demonstrate that MAPK-stimulated IEG induction depends on the mitogen and stress-activated protein kinase (MSK) and its phosphorylation of histone H3 at serine 28 (pH3S28). pH3S28 in IEG promoters in turn recruits Brg1, a BAF60 ATP-dependent chromatin remodelling complex component, initiating gene expression. Without MSK activity and IEG induction, the hypertrophic response is suppressed. These studies provide new mechanistic insights into the role of MAPK pathways in signalling to the epigenome and regulation of gene expression during cardiac hypertrophy.
Experiences of Pediatric Pain Professionals Providing Care during the COVID-19 Pandemic: A Qualitative Study
Authors: Killackey Tieghan , Baerg Krista , Dick Bruce , Lamontagne Christine , Poolacherla Raju , Finley G. Allen , Noel Melanie , Birnie Kathryn A. , Choinière Manon , Pagé M. Gabrielle , Dassieu Lise , Lacasse Anaïs , Lalloo Chitra , Poulin Patricia , Ali Samina , Battaglia Marco , Campbell Fiona , Harris Lauren , Mohabir Vina , Nishat Fareha , Benayon Myles , Jordan Isabel , Stinson Jennifer
Published: February 2022

Chronic pain affects 1 in 5 youth, many of whom manage their pain using a biopsychosocial approach. The COVID-19 pandemic has impacted the way that healthcare is delivered. As part of a larger program of research, this study aimed to understand the impact of the pandemic on pediatric chronic pain care delivery including impact on patients’ outcomes, from the perspective of pediatric healthcare providers. A qualitative descriptive study design was used and 21 healthcare providers from various professional roles, clinical settings, and geographic locations across Canada were interviewed. Using a reflexive thematic analysis approach 3 themes were developed: (1) duality of pandemic impact on youth with chronic pain (i.e., how the pandemic influenced self-management while also exacerbating existing socioeconomic inequalities); (2) changes to the healthcare system and clinical practices (i.e., triaging and access to care); (3) shift to virtual care (i.e., role of institutions and hybrid models of care). These findings outline provider perspectives on the positive and negative impacts of the pandemic on youth with chronic pain and highlight the role of socioeconomic status and access to care in relation to chronic pain management during the pandemic in a high-income country with a publicly funded healthcare system.
Artificial Neural Networks to Predict Sheet Resistance of Indium-Doped Zinc Oxide Thin Films Deposited via Plasma Deposition
Authors: Salimian Ali , Aminishahsavarani Arjang , Upadhyaya Hari
Published: February 2022

We implemented deep learning models to examine the accuracy of predicting a single feature (sheet resistance) of thin films of indium-doped zinc oxide deposited via plasma sputter deposition by feeding the spectral data of the plasma to the deep learning models. We carried out 114 depositions to create a large enough dataset for use in training various artificial neural network models. We demonstrated that artificial neural networks could be implemented as a model that could predict the sheet resistance of the thin films as they were deposited, taking in only the spectral emission of the plasma as an input with the objective of taking a step toward digital manufacturing in this area of material engineering.
Study of Helium Swelling and Embrittlement Mechanisms in SiC Ceramics
Authors: Tynyshbayeva Kymbat , Kadyrzhanov Kayrat , Kozlovskiy Artem , Kuldeyev Yerzhan , Uglov Vladimir , Zdorovets Maxim
Published: February 2022

This work is devoted to the study of the radiation damage kinetics and subsequent embrittlement of the near-surface layer of SiC ceramics subjected to irradiation with low-energy He2+ ions. Interest in these types of ceramics is due to their great prospects for use as structural materials for nuclear power, as well as for use in the creation of protective structures for long-term storage of spent nuclear fuel. During the study, the dependences of changes in the structural, mechanical, strength, and morphological characteristics of SiC ceramics depending on irradiation fluence were obtained. It has been established that the greatest changes in the strength properties are associated with the dominance of the crystal lattice swelling effect in the structure due to an increase in the concentration of implanted helium, and its further agglomeration with the formation of vacancy complexes of the He-V type. A model for changing the structural properties of ceramics irradiated with low-energy He2+ ions based on the change in the contributions of the dislocation density concentration, anisotropic distortion of the crystal lattice, and the effect of swelling as a result of implantation is proposed.
Purely Australian Essential Oils Past and Present: Chemical Diversity, Authenticity, Bioactivity, and Commercial Value
Authors: Sadgrove Nicholas John
Published: February 2022

In this comprehensive commentary, Australian essential oils and their components are listed and discussed in the context of their value to industry and aesthetics. The historic and cultural significance of endemic essential oils is explained. Several promising candidates are identified that have commercial potential and will enter the marketplace in the not-too-distant future. This text elaborates on the current progress in research, and explains the up-to-date view of ‘bioactive,’ with reference to insect repellence, antimicrobial activity, anti-inflammatory activity, and potential toxicity. The concept of chemotypes and chemophenetics is explained in detail to justify why chemically variable species in Australia require standardisation practices to ensure reproducibility of their derived natural products: standardisation practice includes cultivar development and authentication protocols. Thereafter, some of the more significant essential oils are defined and some background information provided. This review concludes with a comprehensive table of aromatic species that were studied by Joseph Brophy over the last 30 years, thereby providing the most comprehensive overview available, on the chemistry of Australian essential oil yielding species.
Myelin-Weighted Imaging Presents Reduced Apparent Myelin Water in Patients with Alzheimer’s Disease
Authors: Lim Seung-Hyun , Lee Jiyoon , Jung Sumin , Kim Bokyung , Rhee Hak Young , Oh Se-Hong , Park Soonchan , Cho Ah Rang , Ryu Chang-Woo , Jahng Geon-Ho
Published: February 2022

The purpose of this study was to investigate myelin loss in both AD and mild cognitive impairment (MCI) patients with a new myelin water mapping technique within reasonable scan time and evaluate the clinical relevance of the apparent myelin water fraction (MWF) values by assessing the relationship between decreases in myelin water and the degree of memory decline or aging. Twenty-nine individuals were assigned to the cognitively normal (CN) elderly group, 32 participants were assigned to the MCI group, and 31 patients were assigned to the AD group. A 3D visualization of the short transverse relaxation time component (ViSTa)-gradient and spin-echo (GraSE) sequence was developed to map apparent MWF. Then, the MWF values were compared between the three participant groups and was evaluated the relationship with the degree of memory loss. The AD group showed a reduced apparent MWF compared to the CN and MCI groups. The largest AUC (area under the curve) value was in the corpus callosum and used to classify the CN and AD groups using the apparent MWF. The ViSTa-GraSE sequence can be a useful tool to map the MWF in a reasonable scan time. Combining the MWF in the corpus callosum with the detection of atrophy in the hippocampus can be valuable for group classification.
Cross-Sectional and Time-Dependent Analyses on Inflammatory Markers following Natural Killer Cell Activity
Authors: Lee Yun-Kyong , Haam Ji-Hee , Cho Sung-Hoon , Kim Young-Sang
Published: February 2022

The function of natural killer (NK) cells in inflammation has not been explored enough in large-scale population studies. The cross-sectional and time-dependent relationship between NK cell activity (NKA) and inflammatory markers was examined. Methods: A total of 7031 subjects were involved in the cross-sectional analyses. Non-linear relationship between NKA and inflammatory indices was analyzed using generalized additive models. The time-dependent changes were analyzed in 1005 subjects with repeated measurement in 3–6 months. The changes in inflammatory markers were analyzed based on the changes in NKA. Results: As NKA reduces to a very low level, the white blood cell (WBC) and neutrophil counts increase sharply, and the lymphocyte count exhibits a slow decline. With increasing NKA larger than about 500 pg/mL, WBC and neutrophil-lymphocyte ratio (NLR) reduces in a mild slope. Among the subjects with repeated measurements, the follow-up NKA was increased with advancing baseline NKA levels. The subjects with a reduction in NKA indicated increment in WBC count, neutrophil count, and NLR, and decrease in lymphocyte count. Conclusions: Very low levels of NKA suggest a high inflammatory immune response. The changes in NKA may interact with the balance between neutrophils and lymphocytes.
Prediction of Wall Heat Fluxes in a Rocket Engine with Conjugate Heat Transfer Based on Large-Eddy Simulation
Authors: Potier Luc , Duchaine Florent , Cuenot Bénédicte , Saucereau Didier , Pichillou Julien
Published: February 2022

Although a lot of research and development has been done to understand and master the major physics involved in cryogenic rocket engines (combustion, feeding systems, heat transfer, stability, efficiency, etc.), the injection system and wall heat transfer remain critical issues due to complex physics, leading to atomization in the subcritical regime and the interactions of hot gases with walls. In such regimes, the fuel is usually injected through a coaxial annulus and triggers the atomization of the central liquid oxidizer jet. This type of injector is often referred to as air-assisted, or coaxial shear, injector, and has been extensively studied experimentally. Including such injection in numerical simulations requires specific models as simulating the atomization process is still out of reach in practical industrial systems. The effect of the injection model on the flame stabilization process and thus on wall heat fluxes is of critical importance when it comes to the design of wall-cooling systems. Indeed, maximizing the heat flux extracted from the chamber can lead to serious gain for the cooling and feeding systems for expander-type feeding cycles where the thermal energy absorbed by the coolant is converted into kinetic energy to drive the turbo-pumps of the feeding system. The methodology proposed in this work to numerically predict the flame topology and associated heat fluxes is based on state-of-the-art methods for turbulent reactive flow field predictions for rocket engines, including liquid injection, combustion model, and wall treatment. For this purpose, high-fidelity Large Eddy Simulation Conjugate Heat Transfer, along with a reduced kinetic mechanism for the prediction of H2/O2 chemistry, liquid injection model LOx sprays, and the use of a specific wall modeling to correctly predict heat flux for large temperature ratio between the bulk flow and the chamber walls, is used. A smooth and a longitudinally ribbed combustor configuration from JAXA are simulated. The coupling strategy ensures a rapid convergence for a limited additional cost compared to a fluid-only simulation, and the wall heat fluxes display a healthy trend compared to the experimental measurements. An increase of heat transfer coherent with the literature is observed when walls are equipped with ribs, compared to smooth walls. The heat transfer enhancement of the ribbed configuration with respect to the smooth walls is coherent with results from the literature, with an increase of around +80% of wall heat flux extracted for the same chamber diameter.
The Asymmetric Impact of Oil Price Shocks on Sectoral Returns in Pakistan: Evidence from the Non-Linear ARDL Approach
Authors: Ali Basit , Khan Dilawar , Shafiq Muhammad , Magda Róbert , Oláh Judit
Published: February 2022

This study explores the asymmetric impact of oil supply and demand shocks on the sectoral stock market returns of Pakistan. For this purpose, the study uses the non-linear autoregressive distributed lag (ARDL) approach based on monthly time series data for four sectors in the Pakistan Stock Exchange over the period 2005–2018. First, the findings of the unit root tests identified that all data series are stationary at first difference. Second, the F-bound test explored that oil supply and demand shocks have a cointegration relationship with sectoral stock market returns. Third, the study explored the asymmetric impact of oil supply shocks (OSS) and oil-specific demand shocks (OSDS) on sectoral returns of commercial banking and the symmetric impact of oil demand shocks (ODS) on sectoral returns of commercial banking. In addition, the asymmetric impact of oil supply shocks (OSS) and oil demand shocks (ODS) on sectoral returns of the power generation, chemical and fertilizer sectors was found. Moreover, the symmetric impact of oil-specific demand shocks (OSDS) on sectoral returns of the power generation, chemical and fertilizer sectors was explored. The study suggests important policy implications for policymakers and investors.
Voltage Pulse Driven VO2 Volatile Resistive Transition Devices as Leaky Integrate-and-Fire Artificial Neurons
Authors: Xu Zhen , Bernussi Ayrton A. , Fan Zhaoyang
Published: February 2022

In a hardware-based neuromorphic computation system, using emerging nonvolatile memory devices as artificial synapses, which have an inelastic memory characteristic, has attracted considerable interest. In contrast, the elastic artificial neurons have received much less attention. An ideal material system that is suitable for mimicking biological neurons is the one with volatile (or mono-stable) resistive change property. Vanadium dioxide (VO2) is a well-known material that exhibits an abrupt and volatile insulator-to-metal transition property. In this work, we experimentally demonstrate that pulse-driven two-terminal VO2 devices behave in a leaky integrate-and-fire (LIF) manner, and they elastically relax back to their initial value after firing, thus, mimicking the behavior of biological neurons. The VO2 device with a channel length of 20 µm can be driven to fire by a single long-duration pulse (>83 µs) or multiple short-duration pulses. We further model the VO2 devices as resistive networks based on their granular domain structure, with resistivities corresponding to the insulator or metallic states. Simulation results confirm that the volatile resistive transition under voltage pulse driving is caused by the formation of a metallic filament in an avalanche-like process, while this volatile metallic filament will relax back to the insulating state at the end of driving pulses. The simulation offers a microscopic view of the dynamic and abrupt filament formation process to explain the experimentally observed LIF behavior. These results suggest that VO2 insulator–metal transition could be exploited for artificial neurons.
Mechanism of Magnetic Nanoparticle Enhanced Microwave Pyrolysis for Oily Sludge
Authors: Qi Hongyuan , Jiang Huayi , You Yanzhen , Hu Juan , Wang Yulong , Wu Zhe , Qi Hongxin
Published: February 2022

In view of the high dielectric constant of magnetic nanoparticles, this paper intends to use it as a new type of microwave absorbing medium to accelerate the microwave pyrolysis process of oily sludge. Microwave thermogravimetric reaction and pyrolysis product staged collection devices were established, respectively. The main stage of pyrolysis process of oily sludge was divided based on the thermogravimetric experiments. Mechanism was studied through the characteristics of pyrolysis products and reaction kinetics simulation. Experimental results showed that the addition of magnetic ZnFe2O4 particle did not change the microwave pyrolysis process of oily sludge and the pyrolysis efficiency could be improved. Pyrolysis process was divided into three stages, rapid heating and water evaporation stage (20~150 °C), light component evaporation stage (150~240 °C) and heavy component cracking stage (240~300 °C). Due to the addition of magnetic ZnFe2O4 particles, the content of C4~C12 increased by 3.5%, and the content of C18+ decreased by 4.1%, indicating that more recombinant components participated in the reaction pyrolysis to form light gas components. The kinetic analysis showed that the activation energy of oily sludge decreased by 36.49% and the pre-exponential factor decreased by 91.39% in stage III, indicating that magnetic nanoparticles had good catalytic activity.
Effects of Air Supply Terminal Devices on the Performance of Variable Refrigerant Flow Integrated Stratum Ventilation System: An Experimental Study
Authors: Yau Yat Huang , Rajput Umair Ahmed , Rajpar Altaf Hussain , Lastovets Natalia
Published: February 2022

A variable refrigerant flow integrated stratum ventilation (VRF-SV) system was proposed as an energy efficient substitute for conventional central cooling systems for buildings. The novel system provided conditioned air to enclosed spaces with high indoor air quality and thermal comfort. This study investigated the effects of different types of ASTDs on the performance of the VRF-SV hybrid system. The performance was experimentally evaluated with five air terminal types, including bar grille, double deflection grille, jet slot, perforated and drum louver diffusers. The evaluation was carried out using standard indices: temperature and velocity distribution, airflow pattern, effective draft temperature (EDT), air distribution performance index (ADPI), thermal sensation vote and comfort feedback survey. The results indicated that the ASTD type had a significant impact on airflow pattern. Furthermore, the bar grille diffuser provided the occupants with greater thermal comfort and acceptable indoor environment. Almost all the EDT values determined in the breathing zone in the case with bar grille diffuser found under the satisfactory range, i.e., −1.2 < K < 1.2. Based on these values, the ADPI for bar grille diffuser was calculated as 92.8%. Thus, the bar grille diffuser is recommended to be installed with the VRF-SV hybrid system in buildings.
Effects of Nitrogen Addition on Microbial Carbon Use Efficiency of Soil Aggregates in Abandoned Grassland on the Loess Plateau of China
Authors: Zhao Xue , Lu Xiaoyue , Yang Jiayi , Zhang Dan , Ren Chengjie , Wang Xiukang , Zhang Xiaoxi , Deng Jian
Published: February 2022

Soil microbial carbon use efficiency (CUE) plays a crucial role in terrestrial C cycling. However, how microbial CUE responds to nitrogen addition and its mechanisms in soil aggregates from abandoned grassland systems remains poorly understood. In this study, we designed a nitrogen (N) addition experiment (0 (N0), 10 (N1), 20 (N2), 40 (N3), 80 (N4) kg N ha−1yr−1) from abandoned grassland on the Loess Plateau of China. Subsequently, the enzymatic stoichiometry in soil aggregates was determined and modeled to investigate microbial carbon composition and carbon utilization. The vegetation and soil aggregate properties were also investigated. Our research indicated that soil microbial CUE changed from 0.35 to 0.53 with a mean value of 0.46 after N addition in all aggregates, and it significantly varied in differently sized aggregates. Specifically, the microbial CUE was higher and more sensitive in macro-aggregates after N addition than in medium and micro-aggregates. The increasing microbial CUE in macro-aggregates was accompanied by an increase in soil organic carbon and microbial biomass carbon, indicating that N addition promoted the growth of microorganisms in macro-aggregates. N addition significantly improved the relative availability of nitrogen in all aggregates and alleviated nutrient limitation in microorganisms, thus promoting microbial CUE. In conclusion, our study indicates that soil microbial CUE and its influencing factors differ among soil aggregates after N addition, which should be emphasized in future nutrient cycle assessment in the context of N deposition.
Resin Transfer Molding of Wood Strand Composite Panels
Authors: Gartner Benjamin , Yadama Vikram , Smith Lloyd
Published: February 2022

The purpose of this study was to develop high-performance wood strand panels for automotive application using resin transfer and compression resin transfer molding technologies. Wood strand preforms bonded with 1%, 5%, and 20% by weight low-density polyethylene and 1% by weight high-density polyethylene were impregnated with resin during the molding process and compared. The results showed that 1% low-density polyethylene is sufficient to bind wood strands into a stable preform for handling and processing. Permeability measurements using a volumetric interpretation of Darcy’s law accounted for resin flow through the thickness of the preform. When compared with previously published data on other natural fiber composites, resin transfer molded wood strand composites generally exhibited superior mechanical properties and significantly greater dimensional stability when exposed to moisture.