bluuebunny/crossref_metadata_2025 huggingface dataset:
Relativistic self-focusing in the interaction of laser beam and plasma with periodical density ripple
Authors: Zhang Geng , Liang Qiuqun , Xia Xiongping
Published: October 2020

In the paper, relativistic self-focusing in the interaction of laser beam and plasma with periodical density ripple has been studied by the applied WKB approximation and higher-order paraxial theory. The result shows that under the influence of relativistic nonlinear effect, the dielectric function shows the fierce oscillational variation with similar periodicity, which then leads to the intense relativistic beam self-focusing along the propagation distance, such self-focusing also presents similar periodic variation. Besides, in the plasma with periodical density ripple, the initial density and the density ripple amplitude have obvious influence on self-focusing. When the two factors increase, then there will be more strength self-focusing. Choosing the appropriate initial density and the periodic density parameter is benefit to the formation of the more stable self-focusing.
THE EFFECT OF LEARNER CHOICE ON L2 TASK ENGAGEMENT
Authors: Nakamura Sachiko , Phung Linh , Reinders Hayo
Published: October 2020

This study examined the effect of choice on EFL learners’ task engagement. Twenty-four Thai university students completed two opinion-gap tasks. In one, they discussed and agreed on three items among given options (+constraint). In the other, they discussed and agreed on three items among the options they generated (−constraint). Spoken interaction and questionnaires were analyzed for behavioral (time on task, words produced, turns), cognitive (negotiation of meaning and form, self-repairs), social (overlaps and turn completion, backchannels), and emotional engagement (anxiety, enjoyment), based on Philp and Duchesne’s multifaceted model. The −constraint task had positive effects on all the cognitive engagement measures, but only one of the behavioral measures (turns) and one of the social measures (overlaps). Learners reported higher anxiety and enjoyment in the −constraint task. The findings highlight the interrelated multidimensional nature of learner task engagement while suggesting pedagogical implications and avenues for future research.
The multifaceted role of mobile technologies as a strategy to combat COVID-19 pandemic
Authors: Teixeira R. , Doetsch J.
Published: October 2020

The coronavirus 2019 (COVID-19) outbreak in China rapidly spread throughout the world, becoming a threatening pandemic with unprecedented consequences. Mobile technologies have led to a revolution in health and their applicability in the context of COVID-19 is promising. In this commentary, we provide an overview of the role that mobile technologies play in the COVID-19 pandemic context and discuss the main issues associated. Four main domains stood out: health communication, prevention, support and research. Strengthening local surveillance systems, geographic contact tracing, support for clinical practice and data collection of real-time longitudinal data at the population level are some of the main advantages of the applications reported so far. The potential conflict to data privacy urges for discussion on their use in a responsible manner. Along with fair regulation and close monitoring of data collection and process, data anonymisation must be a standard and personal data must be deleted after its usage. Preparation is key for effective containment of a public health crisis and learning lessons on the role of mobile technologies is useful for future challenges in global health. It is noteworthy that their use must be driven by an equitable and inclusive orientation, and mostly integrated into an articulated policy to respond to the crisis.
New determinations of tides on the north-western Ross Ice Shelf
Authors: Ray Richard D. , Larson Kristine M. , Haines Bruce J.
Published: October 2020

New determinations of ocean tides are extracted from high-rate Global Positioning System (GPS) solutions at nine stations sitting on the Ross Ice Shelf. Five are multi-year time series. Three older time series are only 2–3 weeks long. These are not ideal, but they are still useful because they provide the only in situ tide observations in that sector of the ice shelf. The long tide-gauge observations from Scott Base and Cape Roberts are also reanalysed. They allow determination of some previously neglected tidal phenomena in this region, such as third-degree tides, and they provide context for analysis of the shorter datasets. The semidiurnal tides are small at all sites, yet M2 undergoes a clear seasonal cycle, which was first noted by Sir George Darwin while studying measurements from the Discovery expedition. Darwin saw a much larger modulation than we observe, and we consider possible explanations - instrumental or climatic - for this difference.
Normative Data for the ELSA-Brasil Neuropsychological Assessment and Operationalized Criterion for Cognitive Impairment for Middle-Aged and Older Adults
Authors: Bertola Laiss , Benseñor Isabela M. , Goulart Alessandra C. , Brunoni Andre R. , Caramelli Paulo , Barreto Sandhi Maria , Giatti Luana , Salvador Larissa , Griep Rosane Harter , Moreno Arlinda B. , Lotufo Paulo A. , Suemoto Claudia K.
Published: October 2020

Objectives:

Normative data should consider sociodemographic diversity for the accurate diagnosis of cognitive impairment. This study aims to provide normative data for a brief neuropsychological battery and present diagnostic criteria for cognitive impairment that could be used in primary care settings.

Methods:

We selected 9618 Brazilian middle-aged and older adults after detailed exclusion criteria to avoid subtle cognitive impairment. We analyzed age, sex, and education influence on cognitive performance. To verify the evidence of criterion validity, we compared the cognitive performance of subjects with and without a depressive episode. Additionally, we verified the percentage of spurious scores under three different cutoffs.

Results:

Age and education had the greatest impact on cognition. Normative scores were provided according to age and education groups. Participants with a depressive episode performed poorer than control subjects. The clinical cutoff of at least two scores below the 7th percentile revealed the adequate percentage of spurious and possible clinical performance.

Conclusions:

The Longitudinal Study on Adult Health (ELSA-Brasil) provided normative data based on a unique selected set of cognitively normal subjects. Normative groups were selected based on age and education, and the battery was sensitive to the presence of a depressive episode. We suggested clinical cutoffs for the tests in this battery that could be used in primary care settings to improve the accurate diagnosis of cognitive impairment.
Machine learning-based broadband GaN HEMT behavioral model applied to class-J power amplifier design
Authors: Cai Jialin , King Justin , Chen Shichang , Wu Meilin , Su Jiangtao , Wang Jianhua
Published: October 2020

A novel, broadband, nonlinear behavioral model, based on support vector regression (SVR) is presented in this paper. The proposed model, distinct from existing SVR-based models, incorporates frequency information into its formalism, allowing the model to perform accurate prediction across a wide frequency band. The basic theory of the proposed model, along with model implementation and the model extraction procedure for radio frequency transistor devices is provided. The model is verified through comparisons with the simulation of an equivalent circuit model, as well as experimental measurements of a 10 W Gallium Nitride (GaN) transistor. It is seen that the efficiency prediction throughout the Smith chart, for varying fundamental and second harmonic loads, across a wideband frequency range, show excellent fidelity to the measured results. Device dc self-biasing is also modelled to allow prediction of power amplifier (PA) efficiency, which is shown to be highly accurate when compared with corresponding measured data. Finally, a class-J PA is constructed and measured across the frequency with a large-signal input tone. The resulting measured and modelled values of key PA performance figures are shown to be in excellent agreement, indicating the model is suitable for broadband PA design.
Self-powered wearable electronics
Authors: Tan Puchuan , Zou Yang , Fan Yubo , Li Zhou
Published: October 2020

Wearable electronics are an essential direction for the future development of smart wearables. Among them, the battery life of wearable electronics is a key technology that limits their development. The proposal of self-powered wearable electronics (SWE) provides a promising solution to the problem of long-term stable working of wearable electronics. This review has made a comprehensive summary and analysis of recent advances on SWE from the perspectives of energy, materials, and ergonomics methods. At the same time, some representative research work was introduced in detail. SWE can be divided into energy type SWE and sensor type SWE according to their working types. Both types of SWE are broadly applied in human–machine interaction, motion information monitoring, diagnostics, and therapy systems. Finally, this article summarizes the existing bottlenecks of SWE, and predicts the future development direction of SWE.
Entzündliche Brusterkrankungen: Klinik und Diagnostik der seltenen Mastitis-Formen
Authors: Vorburger Denise , Frauchiger-Heuer Heike , Dedes Konstantin J.
Published: October 2020

Zusammenfassung. Die häufigsten Ursachen einer Brustentzündung (Mastitis) sind bakteriell, durch die üblichen Keime der Hautflora bedingt. Sie sind sowohl bei stillenden als auch bei nicht stillenden Frauen am häufigsten. Klinische Hinweise und eine standardisierte Diagnostik können zur raschen Erkenntnis der seltenen Mastitis-Formen führen. Eine internistische Grunderkrankung oder der Nachweis seltener Krankheitserreger können hinweisend sein. Der wichtigste diagnostische Schritt in der Erkennung seltener Mastitis-Formen ist jedoch die Gewebegewinnung zur histologischen Aufarbeitung. Das inflammatorische Mammakarzinom gilt es bei allen entzündlichen Brusterkrankungen immer auszuschliessen. Dieses MiniReview liefert eine Übersicht zu den seltenen Mastitis-Formen und zeigt, wie das klinische Bild und die eingeleitete Diagnostik zur richtigen Diagnose führen.
Ungewöhnliche Ursache für ein Nierenversagen bei Patient mit metastasiertem Urothelkarzinom der Harnblase unter palliativer Chemotherapie
Authors: Müller Julia , Kalbermatten Magaya Natalie , Traichel Birgit , Taverna Christian
Published: October 2020

Zusammenfassung. Das Tumorlysesyndrom ist eine potenziell lebensbedrohliche Komplikation von Tumorerkrankungen und deren Behandlung. Meist tritt es bei hochproliferativen hämatologischen Malignomerkrankungen unter zytotoxischer Therapie auf, kann jedoch auch spontan und bei soliden Tumoren auftreten, insbesondere bei bekannter hoher Chemotherapie-Sensitivität (Keimzelltumor, kleinzelliges Lungenkarzinom) und/oder hoher Tumorlast. Der vorliegende Fall beschreibt ein Tumorlysesyndrom bei einem Patienten mit metastasiertem Urothelkarzinom mit lediglich erhöhter Laktatdehydrogenase als mögliches Korrelat einer hohen Tumorlast.
Bat Guano Traces Changes in Agriculture and Hurricane Activity
Authors: Kornei Katherine
Published: October 2020

Researchers hiked and rappeled into two caves in Jamaica to collect over 40 kilograms of excrement.
Hydrology Helps Identify Future Malaria Hot Spots
Authors: Thomasy Hannah
Published: October 2020

Complex hydrological processes—not just the amount of rainfall—help determine where malaria-transmitting mosquitoes can thrive.
Organic Carbon Export and Loss Rates in the Red Sea
Authors: Kheireddine Malika , Dall'Olmo Giorgio , Ouhssain Mustapha , Krokos George , Claustre Hervé , Schmechtig Catherine , Poteau Antoine , Zhan Peng , Hoteit Ibrahim , Jones Burton H.
Published: October 2020

The export and fate of organic carbon in the mesopelagic zone are still poorly understood and quantified due to lack of observations. We exploited data from a biogeochemical‐Argo float that was deployed in the Red Sea to study how a warm and hypoxic environment can affect the fate of the organic carbon in the ocean's interior. We observed that only 10% of the particulate organic carbon (POC) exported survived at depth due to remineralization processes in the upper mesopelagic zone. We also found that POC exported was rapidly degraded in a first stage and slowly in a second one, which may be dependent on the palatability of the organic matter. We observed that apparent oxygen utilization (AOU)‐based loss rates (a proxy of the remineralization of total organic matter) were significantly higher than the POC‐based loss rates, likely because changes in AOU are mainly attributed to changes in dissolved organic carbon. Finally, we showed that POC‐ and AOU‐based loss rates could be expressed as a function of temperature and oxygen concentration. These findings advance our understanding of the biological carbon pump and mesopelagic ecosystem.
Relativistic Electron Microbursts as High‐Energy Tail of Pulsating Aurora Electrons
Authors: Miyoshi Y. , Saito S. , Kurita S. , Asamura K. , Hosokawa K. , Sakanoi T. , Mitani T. , Ogawa Y. , Oyama S. , Tsuchiya F. , Jones S. L. , Jaynes A. N. , Blake J. B.
Published: October 2020

In this study, by simulating the wave‐particle interactions, we show that subrelativistic/relativistic electron microbursts form the high‐energy tail of pulsating aurora (PsA). Whistler‐mode chorus waves that propagate along the magnetic field lines at high latitudes cause precipitation bursts of electrons with a wide energy range from a few kiloelectron volts (PsA) to several megaelectron volts (relativistic microbursts). The rising tone elements of chorus waves cause individual microbursts of subrelativistic/relativistic electrons and the internal modulation of PsA with a frequency of a few hertz. The chorus bursts for a few seconds cause the microburst trains of subrelativistic/relativistic electrons and the main pulsations of PsA. Our simulation studies demonstrate that both PsA and relativistic electron microbursts originate simultaneously from pitch angle scattering by chorus wave‐particle interactions along the field line.
Light-induced irreversible structural phase transition in trilayer graphene
Authors: Zhang Jianyu , Han Jinsen , Peng Gang , Yang Xi , Yuan Xiaoming , Li Yongjun , Chen Jianing , Xu Wei , Liu Ken , Zhu Zhihong , Cao Weiqi , Han Zheng , Dai Jiayu , Zhu Mengjian , Qin Shiqiao , Novoselov Kostya S.
Published: October 2020

A crystal structure has a profound influence on the physical properties of the corresponding material. By synthesizing crystals with particular symmetries, one can strongly tune their properties, even for the same chemical configuration (compare graphite and diamond, for instance). Even more interesting opportunities arise when the structural phases of crystals can be changed dynamically through external stimulations. Such abilities, though rare, lead to a number of exciting phenomena, such as phase-change memory effects. In the case of trilayer graphene, there are two common stacking configurations (ABA and ABC) that have distinct electronic band structures and exhibit very different behaviors. Domain walls exist in the trilayer graphene with both stacking orders, showing fascinating new physics such as the quantum valley Hall effect. Extensive efforts have been dedicated to the phase engineering of trilayer graphene. However, the manipulation of domain walls to achieve precise control of local structures and properties remains a considerable challenge. Here, we experimentally demonstrate that we can switch from one structural phase to another by laser irradiation, creating domains of different shapes in trilayer graphene. The ability to control the position and orientation of the domain walls leads to fine control of the local structural phases and properties of graphene, offering a simple but effective approach to create artificial two-dimensional materials with designed atomic structures and electronic and optical properties.
Early and late effects of electroconvulsive therapy associated with different temporal lobe structures
Authors: Yamasaki Shimpei , Aso Toshihiko , Miyata Jun , Sugihara Genichi , Hazama Masaaki , Nemoto Kiyotaka , Yoshihara Yujiro , Matsumoto Yukiko , Okada Tomohisa , Togashi Kaori , Murai Toshiya , Takahashi Hidehiko , Suwa Taro
Published: October 2020

Recent studies examining electroconvulsive therapy (ECT) have reported that early sessions can induce rapid antidepressant and antipsychotic effects, and the early termination of ECT was reported to increase the risk of relapse. We hypothesized that different neural mechanisms associated with the therapeutic effects of ECT may be involved in the different responses observed during the early and late periods of ECT treatment. We investigated whether these antidepressant and antipsychotic effects were associated with temporally and spatially different regional gray matter volume (GMV) changes during ECT. Fourteen patients with major depressive disorder, with or without psychotic features, underwent 3-Tesla structural magnetic resonance imaging scans before (time point [Tp] 1), after the fifth or sixth ECT session (Tp2), and after ECT completion (Tp3). We investigated the regions in which GMV changed between Tp1 and Tp2, Tp2 and Tp3, and Tp1 and Tp3 using voxel-based morphometry. In addition, we investigated the association between regional GMV changes and improvement in depressive or psychotic symptoms. GMV increase in the left superior and inferior temporal gyrus during Tp1–Tp2 was associated with improvement in psychotic symptoms (P < 0.025). GMV increase in the left hippocampus was associated with improvement of depressive symptoms in Tp2–Tp3 (P < 0.05). Our findings suggest that different temporal lobe structures are associated with early antipsychotic and late antidepressant effects of ECT.
LIM domain proteins Pinch1/2 regulate chondrogenesis and bone mass in mice
Authors: Lei Yiming , Fu Xuekun , Li Pengyu , Lin Sixiong , Yan Qinnan , Lai Yumei , Liu Xin , Wang Yishu , Bai Xiaochun , Liu Chuanju , Chen Di , Zou Xuenong , Cao Xu , Cao Huiling , Xiao Guozhi
Published: October 2020

The LIM domain-containing proteins Pinch1/2 regulate integrin activation and cell–extracellular matrix interaction and adhesion. Here, we report that deleting Pinch1 in limb mesenchymal stem cells (MSCs) and Pinch2 globally (double knockout; dKO) in mice causes severe chondrodysplasia, while single mutant mice do not display marked defects. Pinch deletion decreases chondrocyte proliferation, accelerates cell differentiation and disrupts column formation. Pinch loss drastically reduces Smad2/3 protein expression in proliferative zone (PZ) chondrocytes and increases Runx2 and Col10a1 expression in both PZ and hypertrophic zone (HZ) chondrocytes. Pinch loss increases sclerostin and Rankl expression in HZ chondrocytes, reduces bone formation, and increases bone resorption, leading to low bone mass. In vitro studies revealed that Pinch1 and Smad2/3 colocalize in the nuclei of chondrocytes. Through its C-terminal region, Pinch1 interacts with Smad2/3 proteins. Pinch loss increases Smad2/3 ubiquitination and degradation in primary bone marrow stromal cells (BMSCs). Pinch loss reduces TGF-β-induced Smad2/3 phosphorylation and nuclear localization in primary BMSCs. Interestingly, compared to those from single mutant mice, BMSCs from dKO mice express dramatically lower protein levels of β-catenin and Yap1/Taz and display reduced osteogenic but increased adipogenic differentiation capacity. Finally, ablating Pinch1 in chondrocytes and Pinch2 globally causes severe osteopenia with subtle limb shortening. Collectively, our findings demonstrate critical roles for Pinch1/2 and a functional redundancy of both factors in the control of chondrogenesis and bone mass through distinct mechanisms.
Microbial electroactive biofilms dominated by Geoalkalibacter spp. from a highly saline–alkaline environment
Authors: Yadav Sukrampal , Patil Sunil A.
Published: October 2020

Understanding of the extreme microorganisms that possess extracellular electron transfer (EET) capabilities is pivotal to advance electromicrobiology discipline and to develop niche-specific microbial electrochemistry-driven biotechnologies. Here, we report on the microbial electroactive biofilms (EABs) possessing the outward EET capabilities from a haloalkaline environment of the Lonar lake. We used the electrochemical cultivation approach to enrich haloalkaliphilic EABs under 9.5 pH and 20 g/L salinity conditions. The electrodes controlled at 0.2 V vs. Ag/AgCl yielded the best-performing biofilms in terms of maximum bioelectrocatalytic current densities of 548 ± 23 and 437 ± 17 µA/cm2 with acetate and lactate substrates, respectively. Electrochemical characterization of biofilms revealed the presence of two putative redox-active moieties with the mean formal potentials of 0.183 and 0.333 V vs. Ag/AgCl, which represent the highest values reported to date for the EABs. 16S-rRNA amplicon sequencing of EABs revealed the dominance of unknown Geoalkalibacter sp. at ~80% abundance. Further investigations on the haloalkaliphilic EABs possessing EET components with high formal potentials might offer interesting research prospects in electromicrobiology.
Transcriptome sequencing of cochleae from constant-frequency and frequency-modulated echolocating bats
Authors: Ma Lu , Sun Haijian , Mao Xiuguang
Published: October 2020

Echolocating bats are fascinating for their ability to ‘see’ the world in the darkness. Ultrahigh frequency hearing is essential for echolocation. In this study we collected cochlear tissues from constant-frequency (CF) bats (two subspecies of Rhinolophus affinis, Rhinolophidae) and frequency-modulated (FM) bats (Myotis ricketti, Vespertilionidae) and applied PacBio single-molecule real-time isoform sequencing (Iso-seq) technology to generate the full-length (FL) transcriptomes for the three taxa. In total of 10103, 9676 and 10504 non-redundant FL transcripts for R. a. hainanus, R. a. himalayanus and Myotis ricketti were obtained respectively. These data present a comprehensive list of transcripts involved in ultrahigh frequency hearing of echolocating bats including 26342 FL transcripts, 24833 of which are annotated by public databases. No further comparative analyses were performed on the current data in this study. This data can be reused to quantify gene or transcript expression, assess the level of alternative splicing, identify novel transcripts and improve genome annotation of bat species.
Perception of saccadic reaction time
Authors: Vencato Valentina , Madelain Laurent
Published: October 2020

That saccadic reaction times (SRTs) may depend on reinforcement contingencies has been repeatedly demonstrated. It follows that one must be able to discriminate one’s latencies to adequately assign credit to one’s actions, which is to connect behaviour to its consequence. To quantify the ability to perceive one’s SRT, we used an adaptive procedure to train sixteen participants in a stepping visual target saccade paradigm. Subsequently, we measured their RTs perceptual threshold at 75% in a conventional constant stimuli procedure. For each trial, observers had to saccade to a stepping target. Then, in a 2-AFC task, they had to choose one value representing the actual SRT, while the other value proportionally differed from the actual SRT. The relative difference between the two alternatives was computed by either adding or subtracting from the actual SRT a percent-difference value randomly chosen among a fixed set. Feedback signalling the correct choice was provided after each response. Overall, our results showed that the 75% SRT perceptual threshold averaged 23% (about 40 ms). The ability to discriminate small SRT differences provides support for the possibility that the credit assignment problem may be solved even for short reaction times.
Perceptual inference is impaired in individuals with ASD and intact in individuals who have lost the autism diagnosis
Authors: Jaffe-Dax Sagi , Eigsti Inge-Marie
Published: October 2020

Beyond the symptoms which characterize their diagnoses, individuals with autism spectrum disorder (ASD) show enhanced performance in simple perceptual discrimination tasks. Often attributed to superior sensory sensitivities, enhanced performance may also reflect a weaker bias towards previously perceived stimuli. This study probes perceptual inference in a group of individuals who have lost the autism diagnosis (LAD); that is, they were diagnosed with ASD in early childhood but have no current ASD symptoms. Groups of LAD, current ASD, and typically developing (TD) participants completed an auditory discrimination task. Individuals with TD showed a bias towards previously perceived stimuli—a perceptual process called “contraction bias”; that is, their representation of a given tone was contracted towards the preceding trial stimulus in a manner that is Bayesian optimal. Similarly, individuals in the LAD group showed a contraction bias. In contrast, individuals with current ASD showed a weaker contraction bias, suggesting reduced perceptual inferencing. These findings suggest that changes that characterize LAD extend beyond the social and communicative symptoms of ASD, impacting perceptual domains. Measuring perceptual processing earlier in development in ASD will tap the causality between changes in perceptual and symptomatological domains. Further, the characterization of perceptual inference could reveal meaningful individual differences in complex high-level behaviors.
Absorption, translocation, and effects of Bt Cry1Ac peptides from transgenic cotton to the intercrops and soil functional bacteria
Authors: Zhang Wei , Cao Zhen , Wang Mian , Chen Xiaojiao , Wang Baomin
Published: October 2020

Insecticidal proteins encoded by the truncated genes from Bacillus thuringiensis (Bt) in transgenic crops are released into soil mainly through root exudate and crop residues. In the present study, Bt Cry1Ac protein was hydrolyzed by pronase that was secreted by the soil bacterium Streptomyces griseus. Six peptides were identified as the products of enzymatic hydrolysis by nano liquid chromatography tandem mass spectrometry (LC–MS/MS). One of the six peptides was labeled with radioactive isotope iodine-125 and then purified. The 125I-peptide solution was irrigated to the rhizosphere soil of watermelon seedlings (Citrullus lanatus L.) and wheat seedlings (Triticum aestivum L.), which the two crops usually intercrop with cotton in China. Detection of radioactivity in both plant tissues within one hour proved adsorption, uptake and translocation of the peptide into watermelon and wheat seedlings. Three of the identified peptides were sprayed onto the seedling leaves of watermelon, wheat and maize (Zea mays L.) in the field or the growth chamber. No significant effects on plant growth were observed. These peptides also did not affect growth of organic phosphate-dissolving, nitrogen-fixing, and potassium-dissolving bacteria in the culture. This study provides a new view of GMO risk assessment methodology.
Effects of charge fluctuation and charge regulation on the phase transitions in stoichiometric VO2
Authors: Joshi Siddharth , Smieszek Nicholas , Chakrapani Vidhya
Published: October 2020

Detailed electrical and photoemission studies were carried out to probe the chemical nature of the insulating ground state of VO2, whose properties have been an issue for accurate prediction by common theoretical probes. The effects of a systematic modulation of oxygen over-stoichiometry of VO2 from 1.86 to 2.44 on the band structure and insulator–metal transitions are presented for the first time. Results offer a different perspective on the temperature- and doping-induced IMT process. They suggest that charge fluctuation in the metallic phase of intrinsic VO2 results in the formation of e− and h+ pairs that lead to delocalized polaronic V3+ and V5+ cation states. The metal-to-insulator transition is linked to the cooperative effects of changes in the V–O bond length, localization of V3+ electrons at V5+ sites, which results in the formation of V4+–V4+ dimers, and removal of $$\pi^{*}$$

π

∗

screening electrons. It is shown that the nature of phase transitions is linked to the lattice V3+/V5+ concentrations of stoichiometric VO2 and that electronic transitions are regulated by the interplay between charge fluctuation, charge redistribution, and structural transition.
Brain structural correlates of functional capacity in first-episode psychosis
Authors: Alkan Erkan , Davies Geoff , Greenwood Kathy , L. Evans Simon
Published: October 2020

Impaired functional capacity is a core feature of schizophrenia and presents even in first-episode psychosis (FEP) patients. Impairments in daily functioning tend to persist despite antipsychotic therapy but their neural basis is less clear. Previous studies suggest that volume loss in frontal cortex might be an important contributor, but findings are inconsistent. We aimed to comprehensively investigate the brain structural correlates of functional capacity in FEP using MRI and a reliable objective measure of functioning [University of California, San Diego Performance-Based Skills Assessment (UPSA)]. In a sample of FEP (n = 39) and a well-matched control group (n = 21), we measured cortical thickness, gray matter volume, and white matter tract integrity (fractional anisotropy, FA) within brain regions implicated by previous work. The FEP group had thinner cortex in various frontal regions and fusiform, and reduced FA in inferior longitudinal fasciculus (ILF). In FEP, poorer functional capacity correlated with reduced superior frontal volume and lower FA in left ILF. Importantly, frontal brain volumes and integrity of the ILF were identified as the structural correlates of functional capacity in FEP, controlling for other relevant factors. These findings enhance mechanistic understanding of functional capacity deficits in schizophrenia by specifying the underlying neural correlates. In future, this could help inform intervention strategies.
Synthetic Biology-derived triterpenes as efficacious immunomodulating adjuvants
Authors: Tateno Mizuki , Stone Barbara J. , Srodulski Sarah J. , Reedy Stephanie , Gawriluk Thomas R. , Chambers Thomas M. , Woodward Jerold , Chappell Joe , Kempinski Chase F.
Published: October 2020

The triterpene oil squalene is an essential component of nanoemulsion vaccine adjuvants. It is most notably in the MF59 adjuvant, a component in some seasonal influenza vaccines, in stockpiled, emulsion-based adjuvanted pandemic influenza vaccines, and with demonstrated efficacy for vaccines to other pandemic viruses, such as SARS-CoV-2. Squalene has historically been harvested from shark liver oil, which is undesirable for a variety of reasons. In this study, we have demonstrated the use of a Synthetic Biology (yeast) production platform to generate squalene and novel triterpene oils, all of which are equally as efficacious as vaccine adjuvants based on physiochemical properties and immunomodulating activities in a mouse model. These Synthetic Biology adjuvants also elicited similar IgG1, IgG2a, and total IgG levels compared to marine and commercial controls when formulated with common quadrivalent influenza antigens. Injection site morphology and serum cytokine levels did not suggest any reactogenic effects of the yeast-derived squalene or novel triterpenes, suggesting their safety in adjuvant formulations. These results support the advantages of yeast produced triterpene oils to include completely controlled growth conditions, just-in-time and scalable production, and the capacity to produce novel triterpenes beyond squalene.
Interleukin-26 activates macrophages and facilitates killing of Mycobacterium tuberculosis
Authors: Hawerkamp Heike C. , van Geelen Lasse , Korte Jan , Di Domizio Jeremy , Swidergall Marc , Momin Afaque A. , Guzmán-Vega Francisco J. , Arold Stefan T. , Ernst Joachim , Gilliet Michel , Kalscheuer Rainer , Homey Bernhard , Meller Stephan
Published: October 2020

Tuberculosis-causingMycobacterium tuberculosis(Mtb) is transmitted via airborne droplets followed by a primary infection of macrophages and dendritic cells. During the activation of host defence mechanisms also neutrophils and T helper 1 (TH1) and TH17 cells are recruited to the site of infection. The TH17 cell-derived interleukin (IL)-17 in turn induces the cathelicidin LL37 which shows direct antimycobacterial effects. Here, we investigated the role of IL-26, a TH1- and TH17-associated cytokine that exhibits antimicrobial activity. We found that both IL-26 mRNA and protein are strongly increased in tuberculous lymph nodes. Furthermore, IL-26 is able to directly kill Mtb and decrease the infection rate in macrophages. Binding of IL-26 to lipoarabinomannan might be one important mechanism in extracellular killing of Mtb. Macrophages and dendritic cells respond to IL-26 with secretion of tumor necrosis factor (TNF)-α and chemokines such as CCL20, CXCL2 and CXCL8. In dendritic cells but not in macrophages cytokine induction by IL-26 is partly mediated via Toll like receptor (TLR) 2. Taken together, IL-26 strengthens the defense against Mtb in two ways: firstly, directly due to its antimycobacterial properties and secondly indirectly by activating innate immune mechanisms.
Integrative transcriptomic analysis for linking acute stress responses to squamous cell carcinoma development
Authors: Nguyen Tran N. , Rajapakshe Kimal , Nicholas Courtney , Tordesillas Leticia , Ehli Erik A. , Davis Christel M. , Coarfa Cristian , Flores Elsa R. , Dickinson Sally E. , Curiel-Lewandrowski Clara , Tsai Kenneth Y.
Published: October 2020

Cutaneous squamous cell carcinoma (cuSCC) is the second most common skin cancer and commonly arises in chronically UV-exposed skin or chronic wounds. Since UV exposure and chronic wounds are the two most prominent environmental factors that lead to cuSCC initiation, we undertook this study to test whether more acute molecular responses to UV and wounding overlapped with molecular signatures of cuSCC. We reasoned that transcriptional signatures in common between acutely UV-exposed skin, wounded skin, and cuSCC tumors, might enable us to identify important pathways contributing to cuSCC. We performed transcriptomic analysis on acutely UV-exposed human skin and integrated those findings with datasets from wounded skin and our transcriptomic data on cuSCC using functional pair analysis, GSEA, and pathway analysis. Integrated analyses revealed significant overlap between these three datasets, thus highlighting deep molecular similarities these biological processes, and we identified Oncostatin M (OSM) as a potential common upstream driver. Expression of OSM and its downstream targets correlated with poorer overall survival in head and neck SCC patients. In vitro, OSM promoted invasiveness of keratinocytes and cuSCC cells and suppressed apoptosis of irradiated keratinocytes. Together, these results support the concept of using an integrated, biologically-informed approach to identify potential promoters of tumorigenesis.
Bundle analytics, a computational framework for investigating the shapes and profiles of brain pathways across populations
Authors: Chandio Bramsh Qamar , Risacher Shannon Leigh , Pestilli Franco , Bullock Daniel , Yeh Fang-Cheng , Koudoro Serge , Rokem Ariel , Harezlak Jaroslaw , Garyfallidis Eleftherios
Published: October 2020

Tractography has created new horizons for researchers to study brain connectivity in vivo. However, tractography is an advanced and challenging method that has not been used so far for medical data analysis at a large scale in comparison to other traditional brain imaging methods. This work allows tractography to be used for large scale and high-quality medical analytics. BUndle ANalytics (BUAN) is a fast, robust, and flexible computational framework for real-world tractometric studies. BUAN combines tractography and anatomical information to analyze the challenging datasets and identifies significant group differences in specific locations of the white matter bundles. Additionally, BUAN takes the shape of the bundles into consideration for the analysis. BUAN compares the shapes of the bundles using a metric called bundle adjacency which calculates shape similarity between two given bundles. BUAN builds networks of bundle shape similarities that can be paramount for automating quality control. BUAN is freely available in DIPY. Results are presented using publicly available Parkinson’s Progression Markers Initiative data.
5,7,3ʹ,4ʹ-Tetrahydroxyflav-2-en-3-ol 3-O-glucoside, a new biosynthetic precursor of cyanidin 3-O-glucoside in the seed coat of black soybean, Glycine max
Authors: Yoshida Kumi , Teppabut Yada , Sawaguchi Reo , Nakane Yuhsuke , Hayashi Emi , Oyama Kin-ichi , Nishizaki Yuzo , Goda Yukihiro , Kondo Tadao
Published: October 2020

The seed coat of mature black soybean, Glycine max, accumulates a high amount of cyanidin 3-O-glucoside (Cy3G), which is the most abundant anthocyanin in nature. In the pod, it takes two months for the seed coat color change from green to black. However, immature green beans rapidly adopt a black color within one day when the shell is removed. We analyzed the components involved in the color change of the seed coat and detected a new precursor of Cy3G, namely 5,7,3ʹ,4ʹ-tetrahydroxyflav-2-en-3-ol 3-O-glucoside (2F3G). Through quantitative analysis using purified and synthetic standard compounds, it was clarified that during this rapid color change, an increase in the Cy3G content was observed along with the corresponding decrease in the 2F3G content. Chemical conversion from 2F3G to Cy3G at pH 5 with air and ferrous ion was observed. Our findings allowed us to propose a new biosynthetic pathway of Cy3G via a colorless glucosylated compound, 2F3G, which was oxidized to give Cy3G.
Self-limiting electrospray deposition on polymer templates
Authors: Lei Lin , Gamboa Arielle R. , Kuznetsova Christianna , Littlecreek Sunshine , Wang Jingren , Zou Qingze , Zahn Jeffrey D. , Singer Jonathan P.
Published: October 2020

Electrospray deposition (ESD) applies a high voltage to liquids flowing through narrow capillaries to produce monodisperse generations of droplets down to hundreds of nanometers in diameter, each carrying a small amount of the delivered solute. This deposition method has been combined with insulated stencil masks for fabricating micropatterns by spraying solutions containing nanoparticles, polymers, or biomaterials. To optimize the fabrication process for micro-coatings, a self-limiting electrospray deposition (SLED) method has recently been developed. Here, we combine SLED with a pre-existing patterned polymer film to study SLED’s fundamental behavior in a bilayer geometry. SLED has been observed when glassy insulating materials are sprayed onto conductive substrates, where a thickness-limited film forms as charge accumulates and repels the arrival of additional charged droplets. In this study, polystyrene (PS), Parylene C, and SU-8 thin films of varying thickness on silicon are utilized as insulated spraying substrates. Polyvinylpyrrolidone (PVP), a thermoplastic polymer is sprayed below its glass transition temperature (Tg) to investigate the SLED behavior on the pre-deposited insulating films. Furthermore, to examine the effects of in-plane confinement on the spray, a microhole array patterned onto the PS thin film by laser dewetting was sprayed with dyed PVP in the SLED mode. This was then extended to an unmasked electrode array showing that masked SLED and laser dewetting could be used to target microscale regions of conventionally-patterned electronics.
Prostate cancer risk prediction using a polygenic risk score
Authors: Sipeky Csilla , Talala Kirsi M. , Tammela Teuvo L. J. , Taari Kimmo , Auvinen Anssi , Schleutker Johanna
Published: October 2020

Hereditary factors have a strong influence on prostate cancer (PC) risk and poorer outcomes, thus stratification by genetic factors addresses a critical need for targeted PC screening and risk-adapted follow-up. In this Finnish population-based retrospective study 2283 clinically diagnosed and 455 screen-detected patients from the Finnish Randomised Study of Screening for Prostate Cancer (FinRSPC), 2400 healthy individuals have been involved. Individual genetic risk through establishment of a polygenic risk score based on 55 PC risk SNPs identified through the Finnish subset of the Collaborative Oncological Gene-Environment Study was assessed. Men with PC had significantly higher median polygenic risk score compared to the controls (6.59 vs. 3.83, P < 0.0001). The polygenic risk score above the control median was a significant predictor of PC (OR 2.13, 95% CI 1.90–2.39). The polygenic risk score predicted the risk of PC with an AUC of 0.618 (95% CI 0.60–0.63). Men in the highest polygenic risk score quartile were 2.8—fold (95% CI 2.4–3.30) more likely to develop PC compared with men in the lowest quartile. In the FinRSPC cohort, a significantly higher percentage of men had a PSA level of ≥ 4 ng/mL in polygenic risk score quartile four compared to quartile one (18.7% vs 8.3%, P < 0.00001). Adding the PRS to a PSA-only model contributed additional information in predicting PC in the FinRSPC model. Results strongly suggest that use of the polygenic risk score would facilitate the identification of men at increased risk for PC.
Estimated internal exposure doses due to indoor radiocaesium contamination in residential houses after the Fukushima nuclear accident
Authors: Yoshida-Ohuchi Hiroko , Shinohara Naohide
Published: October 2020

This work first reports the estimation of the internal exposure from ingestion of house dust and inhalation of aerosol, by employing a measured data on 137Cs activities, bioaccessibility (solubility to water and 1 M HCl), and particle size distribution. The house dust and aerosol samples were collected during the actual indoor cleaning by vacuuming and dusting, from 65 houses and buildings in proximity to the Fukushima Daiichi nuclear power plant (FDNPP) (1.6–16.1 km from the FDNPP) during a period from April 2016 to January 2019. Committed effective doses for an adult owing to the ingestion of house dust of 20 mg per day, which adheres to one’s hands through the hand-to-mouth, and those owing to inhalation of aerosol during dusting for 1.5 h while wearing a mask, were calculated using DCAL software for each house or building, as 1.13 µSv and 4.55 µSv as maximum doses, respectively (as of March 2011). Both the committed effective doses, owing to ingestion and inhalation, were inversely correlated with the distance from the FDNPP, and positively correlated with the indoor surface contamination.
Synthesis and application of gas diffusion cathodes in an advanced type of undivided electrochemical cell
Authors: Zaidi S. Z. J. , Luan Y. , Harito C. , Utari L. , Yuliarto B. , Walsh F. C.
Published: October 2020

This paper reports the oxidation of Remazol black B dye by employing iron ions catalyst based gas diffusion cathodes, (GDCs). A GDC was synthesized by using a layer of carbon black and iron ions catalyst for oxygen reduction to hydrogen peroxide. The results demonstrated around 97% decolorization of Remazol black-B dye for 50 min by iron ions catalyst based GDC. The degradation study was performed under electrogenerated hydrogen peroxide at a constant voltage of − 0.6 V vs Hg/HgSO4 in which the rate of degradation was correlated with hydrogen peroxide production. Overall, the GDC’s found to be effective method to degrade the dyes via electro-Fenton.
Specific cyprinid HIF isoforms contribute to cellular mitochondrial regulation
Authors: Chen Jing , Guan Lihong , Zou Ming , He Shunping , Li Dapeng , Chi Wei
Published: October 2020

Hypoxia-inducible factor 1 (HIF-1) functions as a master regulator of the cellular response to hypoxic stress. Two HIF-1α paralogs, HIF-1αA and HIF-1αB, were generated in euteleosts by the specific, third round of genome duplication, but one paralog was later lost in most families with the exception of cyprinid fish. How these duplicates function in mitochondrial regulation and whether their preservation contributes to the hypoxia tolerance demonstrated by cyprinid fish in freshwater environments is not clear. Here we demonstrated the divergent function of these two zebrafish Hif-1a paralogs through cellular approaches. The results showed that Hif-1aa played a role in tricarboxylic acid cycle by increasing the expression of Citrate synthase and the activity of mitochondrial complex II, and it also enhanced mitochondrial membrane potential and ROS production by reducing free Ca2+ in the cytosol. Hif-1ab promoted intracellular ATP content by up-regulating the activity of mitochondrial complexes I, III and IV and the expression of related genes. Furthermore, both the two zebrafish Hif-1a paralogs promoted mitochondrial mass and the expression level of mtDNA, contributing to mitochondrial biogenesis. Our study reveals the divergent functions of Hif-1aa and Hif-1ab in cellular mitochondrial regulation.
Understanding deep learning in land use classification based on Sentinel-2 time series
Authors: Campos-Taberner Manuel , García-Haro Francisco Javier , Martínez Beatriz , Izquierdo-Verdiguier Emma , Atzberger Clement , Camps-Valls Gustau , Gilabert María Amparo
Published: October 2020

The use of deep learning (DL) approaches for the analysis of remote sensing (RS) data is rapidly increasing. DL techniques have provided excellent results in applications ranging from parameter estimation to image classification and anomaly detection. Although the vast majority of studies report precision indicators, there is a lack of studies dealing with the interpretability of the predictions. This shortcoming hampers a wider adoption of DL approaches by a wider users community, as model’s decisions are not accountable. In applications that involve the management of public budgets or policy compliance, a better interpretability of predictions is strictly required. This work aims to deepen the understanding of a recurrent neural network for land use classification based on Sentinel-2 time series in the context of the European Common Agricultural Policy (CAP). This permits to address the relevance of predictors in the classification process leading to an improved understanding of the behaviour of the network. The conducted analysis demonstrates that the red and near infrared Sentinel-2 bands convey the most useful information. With respect to the temporal information, the features derived from summer acquisitions were the most influential. These results contribute to the understanding of models used for decision making in the CAP to accomplish the European Green Deal (EGD) designed in order to counteract climate change, to protect biodiversity and ecosystems, and to ensure a fair economic return for farmers.