bluuebunny/crossref_metadata_2025 huggingface dataset:
Spatial Analysis of Regional and Income Inequality in the United States
Authors: Khan Muhammad Salar , Siddique Abu Bakkar
Published: October 2021

Understanding the spatial or geographical dependence of income inequality and regional inequality is crucial in the study of inequality. This paper employs a multi-scale, multi-mechanism framework to map and analyze historical patterns of regional and income inequality in the United States (US) by using state and regional panel data spanning over a century. To explore the patterns systematically and see the role of spatial partitioning, we organize the data around several established geographical partitions before conducting various geographical information system (GIS) analyses and statistical techniques. We also investigate the spatial dependence of income inequality and regional inequality. We find that spatial autocorrelation exists for both types of inequality in the US. However, the magnitude of spatial dependence for regional inequality is declining whereas it is volatile for income inequality over time. While income inequality has been at its peak in the most recent decades, we also notice that regional inequality is at its lowest point. As for the choice of partitioning, we observe that within inequality dominates for Census Divisions and Bureau of Economic Analysis (BEA) regions. Conversely, we see that between inequality overall contributes the most to the inequality among Census Regions.
Exploring Adolescents’ Understandings of Disability in a Blended Environment of Interactions
Authors: Stylianidou Nayia , Mavrou Katerina
Published: October 2021

Understanding how disability is socially constructed is important for establishing inclusive schools, since the ways in which educators and non-disabled nondisabled students understand disability affect their actions towards disabled students. As social interaction today is very often an interplay between online and offline communication activities, this study aimed to explore how young adolescents in Cyprus construct the concept of disability in a blended environment of interactions. A qualitative research approach was adopted involving focus groups with 18 adolescents, online and face to face semi-structured semistructured individual interviews, participant observation on a Facebook group set up for the purposes of the study, and the first author’s reflective diary. A combination of thematic analysis, critical discourse analysis and analytic elements from the field of visual semiotics were used for data analysis. Data analysis indicated that adolescents understand disability mostly through medical and charity models. Based on the data, these understandings arise from the construction of disabled people’s multimodal ‘invisibility/absence’, in both online and offline contexts of adolescents’ everyday reality. Suggestions for further research in disability constructions in blended environments, and implications for the field of inclusive education concerning teachers and pre-service teachers’ educational practice are discussed.
Thermal Calculations of Four-Row Plate-Fin and Tube Heat Exchanger Taking into Account Different Air-Side Correlations on Individual Rows of Tubes for Low Reynold Numbers
Authors: Marcinkowski Mateusz , Taler Dawid , Taler Jan , Węglarz Katarzyna
Published: October 2021

Currently, when designing plate-fin and tube heat exchangers, only the average value of the heat transfer coefficient (HTC) is considered. However, each row of the heat exchanger (HEX) has different hydraulic–thermal characteristics. When the air velocity upstream of the HEX is lower than approximately 3 m/s, the exchanged heat flow rates at the first rows of tubes are higher than the average value for the entire HEX. The heat flow rate transferred in the first rows of tubes can reach up to 65% of the heat output of the entire exchanger. This article presents the method of determination of the individual correlations for the air-side Nusselt numbers on each row of tubes for a four-row finned HEX with continuous flat fins and round tubes in a staggered tube layout. The method was built based on CFD modelling using the numerical model of the designed HEX. Mass average temperatures for each row were simulated for over a dozen different airflow velocities from 0.3 m/s to 2.5 m/s. The correlations for the air-side Nusselt number on individual rows of tubes were determined using the least-squares method with a 95% confidence interval. The obtained correlations for the air-side Nusselt number on individual rows of tubes will enable the selection of the optimum number of tube rows for a given heat output of the HEX. The investment costs of the HEX can be reduced by decreasing the tube row number. Moreover, the operating costs of the HEX can also be lowered, as the air pressure losses on the HEX will be lower, which in turn enables the reduction in the air fan power.
An Economic Assessment of the Impact on Agriculture of the Proposed Changes in EU Biofuel Policy Mechanisms
Authors: Gradziuk Piotr , Jończyk Krzysztof , Gradziuk Barbara , Wojciechowska Adrianna , Trocewicz Anna , Wysokiński Marcin
Published: October 2021

In Poland, rapeseed production has been the fastest growing branch of plant production since 2000. Rapeseed yields have increased 2.5 times in the last 20 years. The main reason for this trend was the implementation of obligations resulting from legal acts by Member States relating to increasing the share of RES in the structure of primary energy production, and in particular relating to the share of biofuels in fuels used in transport. In Poland in the years 2010–2020, about 1.0–1.6 million tonnes of rape seeds were used for this purpose annually. Due to the fact that biofuel production competes for raw materials with the food economy, at the end of the first decade of the 21st century, many representatives of various circles intensified their voices, calling for withdrawal from the policy supporting the biofuel sector, which may have resulted in a decrease in oilseed plant cultivation areas. As a result of the research conducted here, it was determined that the place of oilseed rape in the sowing structure will be taken by rye, triticale and, on good soils, by wheat. Compared to rape, their production is characterised by lower income per 1 ha; in the years 2013–2019, these differences amounted to: wheat—8 EUR, triticale—102.3 EUR, and rye—168 EUR. This situation will deteriorate the value cereal cultivation sites and will result in a decrease in their yields. On the basis of the conducted research, the estimated value of rape as a forecrop for wheat, triticale, and rye was, respectively: 103.7; 64.6 and 46.7 EUR. An additional advantage of oilseed rape is that it is an excellent bee resource and is classified as a commodity crop, i.e., one from which significant amounts of honey can be obtained, with a net value of EUR 55 per hectare. In addition, in many agricultural holdings, as a result of forecasted changes in plant production, there will be an accumulation of field work during the harvest period, which will also affect the worse use of machinery and storage areas. The consequence of increasing the area under which cereal crops and their supply can grow may be the decline in production profitability and thus the income situation of farms, but this will be assessed at the next stage of research.
Three-Dimensional Thermal Simulations of 18650 Lithium-Ion Batteries Cooled by Different Schemes under High Rate Discharging and External Shorting Conditions
Authors: Li Yang , Zhou Zhifu , Zhao Jian , Hao Liang , Bai Minli , Li Yulong , Liu Xuanyu , Li Yubai , Song Yongchen
Published: October 2021

In this work, three-dimensional thermal simulations of single 18650 lithium-ion battery cell and 75 V lithium-ion battery pack composed of 21 18650 battery cells are performed based on a multi-scale multi-domain (MSMD) battery modeling approach. Different cooling approaches’ effects on 18650 lithium-ion battery and battery pack thermal management under fast discharging and external shorting conditions are investigated and compared. It is found that for the natural convection, forced air cooling, and/or mini-channel liquid cooling approaches, the temperature of battery cell easily exceeds 40 °C under 3C rate discharging condition. While under external shorting condition, the temperature of cell rises sharply and reaches the 80 °C in a short period of time, which can trigger thermal runaway and may even lead to catastrophic battery fire. On the other hand, when the cooling method is single-phase direct cooling with FC-72 as coolant or two-phase immersed cooling by HFE-7000, the cell temperature is effectively limited to a tolerable level under both high C rate discharging and external shorting conditions. In addition, two-phase immersed cooling scheme is found to lead to better temperature uniformity according to the 75 V battery pack simulations.
Sheet Barrier and Intubating Stylet
Authors: Tsai Phil B. , Luk Hsiang-Ning
Published: October 2021

Coronavirus disease 2019 (COVID-19), a respiratory syndrome caused by SARS-CoV-2, can be transmitted through respiratory droplets and aerosols of droplet nuclei. Aerosol-generating medical procedures (AGMP) are needed to take care of critically ill patients but place health care providers at risk of infection. With limited supplies of personal protective equipment (PPE), barrier systems were developed to help protect health care providers during tracheal intubation. The video intubating stylet shows promise to become the preferred intubation device in conjunction with plastic sheet barriers during the COVID-19 pandemic.
Silicon Micro-Strip Detectors
Authors: Landi Gregorio , Landi Giovanni E.
Published: October 2021

Silicon micro-strip detectors are fundamental tools for the high energy physics. Each detector is formed by a large set of parallel narrow strips of special surface treatments (diode junctions) on a slab of very high quality silicon crystals. Their development and use required a large amount of work and research. A very synthetic view is given of these important components and of their applications. Some details are devoted to the basic subject of the track reconstruction in silicon strip trackers. Recent demonstrations substantially modified the usual understanding of this argument.
Lean Pattern in an Altitude Range Shift of a Tree Species: Abies pinsapo Boiss.
Authors: González-Hernández Antonio , Nieto-Lugilde Diego , Peñas Julio , Alba-Sánchez Francisca
Published: October 2021

Organisms modify their geographical distributions in response to changes in environmental conditions, or modify their affinity to such conditions, to avoid extinction. This study explored the altitudinal shift of Abies pinsapo Boiss. in the Baetic System. We analysed the potential distribution of the realised and reproductive niches of A. pinsapo populations in the Ronda Mountains (Southern Spain) by using species distribution models (SDMs) for two life stages within the current populations. Then, we calculated the species’ potential altitudinal shifts and identified the areas in which the processes of persistence and migration predominated. The realised and reproductive niches of A. pinsapo are different to one another, which may indicate a displacement in its altitudinal distribution owing to changes in the climatic conditions of the Ronda Mountains. The most unfavourable conditions for the species indicate a trailing edge (~110 m) at the lower limit of its distribution and a leading edge (~55 m) at the upper limit. Even though the differences in the altitudinal shifts between the trailing and leading edges will not cause the populations to become extinct in the short term, they may threaten their viability if the conditions that are producing the contraction at the lower limit persist in the long term.
Review on Urban Forests and Trees as Nature-Based Solutions over 5 Years
Authors: Kong Xiangqi , Zhang Xinna , Xu Chengyang , Hauer Richard J.
Published: October 2021

As human living environments face increasing challenges with resilience, the concept of nature-based solutions (NBS) was proposed in recent years as a way to promote sustainable living in urban environments. Urban forests and trees play important roles in urban ecosystems, while their potential as an NBS is promising. A bibliometric analysis was first conducted to explore the research pattern of NBS in urban environments. Studies of urban forest and tree-based green infrastructure in NBS research were further investigated using a systematic literature review method. The initial studies on NBS have increased since 2015 with 493 documents published from 142 sources in over 70 countries and regions. Keyword analysis showed green infrastructure had a rather high frequency of utility and received considerable attention. As for urban forests as nature-based solutions (UF-NBS) research, the most prominent study approaches used at different scales and the main benefits and typologies of urban forest studied in the articles were identified. UF-NBS research is still relatively scarce at present. Despite the role of urban forest and trees in addressing environmental challenges being well recognized, UF-NBS studies still need to be conducted in a more comprehensive context, taking social and economic aspects into account.
Exploring the Knowledge and Perceptions of Local Communities on Illegal Hunting: Long-Term Trends in a West African Protected Area
Authors: Afriyie Jerry Owusu , Asare Michael Opare , Hejcmanová Pavla
Published: October 2021

Local communities in rural areas are dependent on hunting for their livelihoods and rely on their knowledge to understand wildlife ecology. Their knowledge and perceptions may be vital for forming effective and sustainable management plans related to wildlife conservation. We aimed to examine perceptions of local people living inside (n = 153 households) and outside (n = 178 households) the Kogyae Strict Nature Reserve (KSNR, Ghana) regarding bushmeat prevalence and long-term trends in illegal hunting, and to explore people’s knowledge about hunting tools, species, and reasons to hunt illegally. Perceptions of bushmeat sightings and illegal hunting trends were influenced by living inside or outside the protected area, gender, and residence time. Residents living inside the reserve perceived bushmeat and hunting as frequently present in their environment mainly due to frequent sightings of bushmeat and other wildlife products, while people living adjacent to the KSNR were more knowledgeable about the decrease in illegal hunting trends, probably because of awareness about penalties and biodiversity conservation. Furthermore, the perceptions of most residents about the decrease in hunting over time were validated by long-term KSNR law enforcement data. The perception in local communities that snares were the commonest form of hunting equipment used was also consistent with the ranger-based monitoring data. The need for money, bushmeat, unemployment, and retaliatory killings were the main drivers for illegal hunting. Our findings indicated that local people’s knowledge can have a valid relevance in protected area management and may assist in developing effective conservation strategies and in overall improvement of local socio-ecological systems.
Rumor Detection Based on Attention CNN and Time Series of Context Information
Authors: Peng Yun , Wang Jianmei
Published: October 2021

This study aims to explore the time series context and sentiment polarity features of rumors’ life cycles, and how to use them to optimize the CNN model parameters and improve the classification effect. The proposed model is a convolutional neural network embedded with an attention mechanism of sentiment polarity and time series information. Firstly, the whole life cycle of rumors is divided into 20 groups by the time series algorithm and each group of texts is trained by Doc2Vec to obtain the text vector. Secondly, the SVM algorithm is used to obtain the sentiment polarity features of each group. Lastly, the CNN model with the spatial attention mechanism is used to obtain the rumors’ classification. The experiment results show that the proposed model introduced with features of time series and sentiment polarity is very effective for rumor detection, and can greatly reduce the number of iterations for model training as well. The accuracy, precision, recall and F1 of the attention CNN are better than the latest benchmark model.
Optimization of the Frying Temperature and Time for Preparation of Healthy Falafel Using Air Frying Technology
Authors: Fikry Mohammad , Khalifa Ibrahim , Sami Rokkaya , Khojah Ebtihal , Ismail Khadiga Ahmed , Dabbour Mokhtar
Published: October 2021

Air-frying is an innovative technique for food frying that uses hot air circulation to prepare healthy products. The objectives of this study were to establish simplified models to reflect the efficacy of the air frying process at varying temperatures and times on the quality attributes of falafel, and to optimize the frying conditions for producing air-fried falafel. Moisture content, color, fat content, hardness, and sensory evaluation of the fried falafel were analyzed under varied temperatures (140 °C, 170 °C, and 200 °C) and time periods (5 min, 10 min, and 15 min). Statistical analysis was then applied to obtain the best fit model that can describe the properties of fried falafel. Results indicated that moisture content, fat content, and L*-value of air-fried falafel were adversely related to the frying temperature and time, but the hardness and ΔE of fried falafel were increased as the frying temperature and time increased. Moreover, an increase followed by a decrease was shown for the appearance, aroma, crispness, taste, and overall preference scores with the increase in frying temperature and time. The regression analysis showed that the proposed models could be properly used for predicting the properties of the fried falafel. In addition, the overlaid plots resulted in the optimum frying temperature of 178.8 °C and time of 11.1 min. Interestingly, the fat content of the air-fried falafel reduced by 45% at optimal frying conditions compared with that for the deep-fat fried one at 180 °C for 7 min (control). In comparison, the air-fried falafel was lower in fat content, higher in hardness with more acceptable appearance and crispness scores than deep-fat fried falafel. Such information could be beneficial to the manufacturers of the falafel to produce an optimal and healthy product.
Unauthorized Food Manipulation as a Criminal Offense: Food Authenticity, Legal Frameworks, Analytical Tools and Cases
Authors: Jurica Karlo , Brčić Karačonji Irena , Lasić Dario , Bursać Kovačević Danijela , Putnik Predrag
Published: October 2021

Food fraud is a criminal intent motivated by economic gain to adulterate or misrepresent food ingredients and packaging. The development of a reliable food supply system is at great risk under globalization, but Food Business Operators (FBOs) have a legal obligation to implement and maintain food traceability and quality at all stages of food production, processing, and distribution. Incidents of food fraud have a strong negative impact on consumer confidence in the food industry. Therefore, local and international regulatory mechanisms are established to prevent or mitigate food fraud. This review brings new perspectives linking EU and US legislation, as well as new definitions and descriptions of the criminal aspect of food fraud incidents. It also describes certain new insights into the application of state-of-the-art methods and techniques that provide valuable tools for geographic, botanical, or other chemical markers of food authenticity. The review also provides an overview of the most common cases of food fraud worldwide from 2010 to 2020. Further research is needed to support the development of predictive models for innovative approaches to adulteration, especially when some valuable nutrients are replaced by toxic ingredients. A possible solution to minimize food fraud incidents is to increase the level of risk-based inspections, establish more productive monitoring and implementation of food protection systems in the supply chain, and implement better ingredient control and certification. National and international (e.g., regional) police offices for food fraud should be introduced, possessing knowledge and skills in food, food safety, food processing, and food products, as initial positive results have emerged in some countries.
The Condorcet Jury Theorem with Information Acquisition
Authors: Chen Jun
Published: October 2021

We analyze a committee decision in which individuals with common preferences are uncertain which of two alternatives is better for them. Members can acquire costly information. Private signals and information choice are both continuous. As is consistent with Down’s rational ignorance hypothesis, each member acquires less information in a larger committee and tends to acquire zero information when the committee size goes to infinity. However, with more members, a larger committee can gather more aggregate information in equilibrium. The aggregate information is infinite with the size going to infinity if and only if marginal cost at “zero information acquisition” is zero. When the marginal cost at “zero information acquisition” is positive, the probability of making an appropriate decision tends to be less than one.
COVID-19 and the Gastrointestinal Tract
Authors: Mucino-Bermejo María-Jimena
Published: October 2021

Since it was discovered at the end of 2019; the pandemic of coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has made a serious public health threat worldwide, with over 175 million confirmed cases reported globally. Even when COVID-19 was initially considered a respiratory disease, it was actually known to be multisystemic, with gastrointestinal involvement a common clinical finding. Furthermore, COVID-19 may affect patients with gastrointestinal comorbidities, being the clinical intersectionality of utmost interest for gastroenterologists; critical care physicians and all the healthcare team taking care of COVID-19 patients. The present article presents a brief review of the reported gastrointestinal manifestations of COVID-19 disease in both previously healthy individuals and in patients with gastrointestinal comorbidities.
Probabilistic Failure Estimation of an Oblique Loaded Footing Settlement on Cohesive Geomaterials with a Modified Cam Clay Material Yield Function
Authors: Savvides Ambrosios-Antonios , Papadrakakis Manolis
Published: October 2021

In this work, a quantitative uncertainty estimation of the random distribution of the soil material properties to the probability density functions of the failure load and failure displacements of a shallow foundation loaded with an oblique load is portrayed. A modified Cam Clay yield constitutive model is adopted with a stochastic finite element model. The random distribution of the reload path inclination κ, the critical state line inclination c of the soil and the permeability k of the Darcian water flow relation, has been assessed with Monte Carlo simulations accelerated by using Latin hypercube sampling. It is proven that both failure load and failure displacements follow Gaussian normal distribution despite the excessive non-linear behaviour of the soil. In addition, as the obliquity increases the mean value of failure load and the failure displacement always increases. The uncertainty of the output failure stress with the increase of the obliquity of the load remains the same. The failure spline of clays can be calculated within an acceptable accuracy with the proposed numerical scheme in every possible geometry and load conditions, considering the obliquity of the load in conjunction with non-linear constitutive relations.
Body Image, Anxiety, and Bulimic Behavior during Confinement Due to COVID-19 in Mexico
Authors: Pineda-García Gisela , Serrano-Medina Aracely , Ochoa-Ruíz Estefanía , Martínez Ana Laura
Published: October 2021

Background: The potential impact of the COVID-19 pandemic on weight, shape-related appearance behaviors (body image dissatisfaction), and bulimic symptoms in nonclinical participants is poorly evaluated. This study aimed to identify the relationship between labor status, confinement degree due to COVID-19, dissatisfaction with body image, and anxiety and to discover its effect on bulimic behavior in Mexican adults. Methods: A cross-sectional study was conducted with a non-probabilistic sample of 276 participants via an online survey. Questions addressed their anxiety, body image dissatisfaction, and bulimic behavior. Results: The path analysis showed direct effects on the confinement degree (β = −0.157); of the labor situation (β = −0.147) and gender (β = 0.129) on anxiety; of dissatisfaction on bulimic behavior (β = 0.443) and anxiety about bulimic behavior (β = 0.184); and dissatisfaction (β = 0.085). Conclusions: The confinement, gender, and labor status are predictors of anxiety, while anxiety and body dissatisfaction directly influence bulimic behavior.
Energy Efficiency Measures Applied to Heritage Retrofit Buildings: A Simulated Student Housing Case Study in Vienna
Authors: Elnagar Essam , Munde Simran , Lemort Vincent
Published: October 2021

One pavilion was selected for deep retrofitting from the Otto Wagner area situated in the west of Vienna. The retrofitting process involves sustainable and energy-efficient construction to improve the energy performance and energy production potential of the building while preserving the cultural heritage and significance. This four-story pavilion was re-designed according to the proposed regulations of a net positive energy university building to become a student residence. Architectural, building envelope, and engineering interventions along with various changes were simulated through the Sefaira tool in the SketchUp model. These included: optimization of the U-values of the roof, walls, and floor; the addition of different layers of sustainable energy-efficient insulation materials to decrease the overall energy demand. The specific energy demands for heating, cooling, and lighting were decreased in the proposed model to reduce the total energy use intensity from 248.9 kWh/(m2 year) to 54.3 kWh/(m2 year) resulting in a 78.2% reduction. The main goal of this study is to try and achieve a net positive energy status building as part of the Otto Wagner area by improving the building envelope and integrating renewable energies. A total of 22.5% of the annual energy consumption was generated by the designed PV system. The selected building achieved the passive house standards in Austria by optimizing the energy performance with the proposed energy efficiency measures.
Risk Factors of Eye Complications in Patients Treated in the Intensive Care Unit
Authors: Płaszewska-Żywko Lucyna , Sega Aurelia , Bukowa Agnieszka , Wojnar-Gruszka Katarzyna , Podstawa Marcelina , Kózka Maria
Published: October 2021

In critically ill patients, normal eye protection mechanisms, such as tear production, blinking, and keeping the eye closed, are impaired. In addition, many other factors related to patients’ severe condition and treatment contribute to ocular surface disease. Reducing risk factors and proper eye care can have a significant impact on incidences of eye complications and patient quality of life after discharge from the intensive care unit (ICU). The aim of the study was to determine risk factors for ocular complication, especially those related to nursing care. The study was conducted in the ICU of a university hospital. Methods for estimating and analyzing medical records were used. The patient’s evaluation sheet covering 12 categories of risk factors for eye complications was worked out. The study group included 76 patients (34 patients with injuries and 42 without injuries). The Shapiro–Wilk test, the Spearman’s rank correlation test, the Mann–Whitney U test and the Friedman’s ANOVA test were used. The level of significance was set at α = 0.05. The most important risk factors for eye complications in the study group were: lagophthalmos (p < 0.001), sedation (p < 0.01), use of some cardiological drugs and antibiotics (p < 0.01), mechanical ventilation (p < 0.05), use of an open suctioning system (p < 0.01), presence of injuries (p < 0.01) including craniofacial trauma (p < 0.001), high level of care intensity (p < 0.01), failure to follow eye care protocol (p < 0.001), length of hospitalization at the ICU (p < 0.001), and the frequency of ophthalmological consultations (p < 0.001). There was no correlation between the incidence of these complications and the age and gender of the patients. The exposure of critically ill patients to eye complications was high. It is necessary to disseminate protocols and guidelines for eye care in ICU patients to reduce the risk factors.
Toothbrushes as a Source of DNA for Gender and Human Identification—A Systematic Review
Authors: Sujatha Govindarajan , Priya Veeraraghavan Vishnu , Dubey Alok , Mujoo Sheetal , Sulimany Ayman M. , Omar Tawhari Ali Mohammed , Mokli Lujain Khalawi , Mohana Arwa Jaber , Varadarajan Saranya , Balaji Thodur Madapusi , Raj A. Thirumal , Patil Shankargouda
Published: October 2021

Background: Few studies have reported the use of toothbrushes as a reliable source of DNA for human or gender identification. The present systematic review with the available information was conducted to answer the focus question “Is a toothbrush a reliable source of DNA for human or gender identification?”. Methods: The keyword combination “Toothbrush” and “DNA” was used to search databases including MEDLINE, Scopus, and Web of Science along with a manual search of reference lists of relevant articles. Duplicates and irrelevant articles were excluded, and the remaining articles were fully read for the final selection of articles. The risk of bias of the included studies was evaluated using the Appraisal tool for Cross-Sectional Studies (AXIS tool). Results: Of the 130 articles obtained, 122 duplicates or irrelevant articles were eliminated. Following the full-text reading of eight articles, five articles were selected based on eligibility criteria. The five studies reported that a toothbrush is a good source of DNA irrespective of the time interval. In a few studies some samples were not sufficient for complete DNA profiling due to factors such as the method of DNA extraction. Conclusion: Although a toothbrush is an excellent source of DNA for human and gender identification, future studies with a larger sample size, appropriate control group, and standardized technique of DNA extraction need to be conducted. Additionally, factors influencing the quantity and quality of DNA in toothbrushes need to be determined with standardized techniques.
Predicting Psychosocial Health of Children and Adolescents with Obesity in Germany: The Underappreciated Role of Physical Fitness
Authors: Eisenburger Nina , Friesen David , Haas Fabiola , Klaudius Marlen , Schmidt Lisa , Vandeven Susanne , Joisten Christine
Published: October 2021

Background: The aim of this study was to analyze the inhibitory and promotive factors of psychosocial health in the context of childhood obesity, incorporating physical fitness as an additional, potentially relevant predictor. Methods: The sample comprised cross-sectional data of 241 children and adolescents with obesity and overweight from the German Children’s Health InterventionaL TriaL III program (12.5 ± 2.1 years; 51.9% girls). Demographics and lifestyle patterns were assessed via parent reports. Anthropometric data and physical fitness in relation to body weight (W/kg) were measured. Children and adolescents completed standardized questionnaires (GW-LQ-KJ, FSK-K) to assess health-related quality of life (HRQOL) and five dimensions of self-concept (scholastic, social, physical, behavioral, and self-worth). Results: Multiple linear regression analysis showed that HRQOL was significantly related to relative physical fitness (W/kg; β = 0.216, p = 0.011) as were scholastic (β = 0.228, p = 0.008) and social self-concept (β = 0.197, p = 0.023). Increasing body mass index (BMI) Z-scores, age, physical activity (hours/day), low parental educational levels, and/or migration background were negatively associated with three subdomains of self-concept (physical, behavioral, self-worth; all p < 0.05). Conclusion: The results emphasize BMI Z-scores, age, physical activity, migration background, and parents’ educational level as relevant predictors of psychosocial health in the context of childhood obesity. Additionally, this study adds physical fitness as a key determinant of HRQOL and self-concept. To enable the development of more effective weight management, therapeutic strategies should therefore consider addressing these aspects and improving physical fitness in particular not only for weight loss but also to strengthen psychosocial health.
Blood Transfusion Knowledge among Nurses in Malaysia: A University Hospital Experience
Authors: Mohd Noor Noor Haslina , Saad Noor Hafiza , Khan Mohammad , Hassan Mohd Nazri , Ramli Marini , Bahar Rosnah , Mohamed Yusoff Shafini , Iberahim Salfarina , Wan Ab Rahman Wan Suriana , Zulkafli Zefarina , Islam Md Asiful
Published: October 2021

Blood transfusion is a fundamental and life-saving procedure where the consequence of errors can be fatal. Nurses’ knowledge plays an essential role in ensuring quality and safety in blood transfusion. The objective of this study was to assess blood transfusion-associated knowledge of tertiary hospital nurses on the east coast of Malaysia. This was a cross-sectional study with 200 registered nurses involved in blood transfusion procedures at Hospital Universiti Sains Malaysia. The knowledge of the nurses was evaluated by using the routine blood transfusion knowledge questionnaire based on five parts, and <50%, 50–74%, or ≥75% of the knowledge was considered as poor, moderate, or high, respectively. Based on the scoring system, the overall knowledge of blood transfusion among Malaysian nurses (33.2 ± 8.4 years) was estimated to be 54.9 ± 7.6%. In individual items, the scoring was 81.0%, 45.4%, 49.2%, 63.0%, and 90.0% in knowledge prior to blood transfusion, on pre-transfusion, on post-transfusion, on complications, and on transfusion policy, respectively. The findings of this study indicated that most of the nurses’ overall knowledge of blood transfusion was at a moderate level; therefore, training courses and continuous medical education are warranted to improve knowledge and skills of the nurses to ensure good practices of blood transfusion.
Transdisciplinary Online Health Assessment of an Artisanal and Small-Scale Gold Mining Community during the COVID-19 Pandemic in the Mandalay Region of Myanmar
Authors: Kyaw Win Thiri , Myint Yee Mon , Kuang Xiaoxu , Sakakibara Masayuki
Published: October 2021

Artisanal and small-scale gold mining (ASGM) has a known negative effect on the community’s health; therefore, assessment to monitor community health is essential to detect any issues and enable early treatment. Because ASGM-related health issues are complex and cannot be addressed effectively with a traditional one-time health assessment alone, both long-term and regular health assessments using a transdisciplinary approach should be considered. In response to this need, we designed an online health assessment tool as a reference for a future long-term health assessment system. An online video interview was conducted with 54 respondents living in the ASGM area of Chaung Gyi Village, Thabeikkyin Township, Mandalay Region, Myanmar, via a social networking service application. The tool was used to evaluate community health during the coronavirus 2019 pandemic, including mercury intoxication symptoms, mining-related diseases, and other diseases. Results show that persons working in mining versus non-mining occupations had a greater prevalence of pulmonary diseases, such as pulmonary tuberculosis, silicosis, and bronchial asthma, in addition to malaria. Based on these findings, online health assessment using a transdisciplinary approach can be recommended as an effective tool for sustainable and long-term health assessment of ASGM-related disease and should be performed regularly following physical health surveys.
Psychological Changes and Cancer Occurrence in Seoul Citizens Due to Changes in Fine Dust Concentration before Seoul Fine Dust Policy
Authors: Choi Kilyong , Yang Wonho
Published: October 2021

Background: Particulate matter and urban air pollution affect the human body and can lead to death. Epidemiological studies should consider exposure to pollutants and the diverse responses of individuals, depending on their sensitivity to the pollutants. Methods: In this study, air pollution measurements were obtained hourly at measuring stations operated by national and local governments to increase the reliability of the measured values. A β-ray absorption method was used to analyze the measurements of fine dust and determine the particulate matter content. Results: The air pollution data were log-linear, thereby enabling a comparison of data from different time periods. The comparison was made by focusing on the period of the implementation of Seoul’s fine dust policy. It was observed that the cancer incidence rate decreased after the implementation of the policy. The data on individual characteristics were obtained from a survey of Seoul citizens conducted from 2015 to 2016 using indicators such as quality of life and the social trust of Seoul citizens. Conclusion: The survey on the living environment and residence indicated that 80% of the heads of households were men. Women had a greater dissatisfaction than men with their residential, economic, and social environments. The survey questions on well-being were related to elements of internal and external environments, such as air pollution, noise, and fine dust.
A Novel Invariant Based Commutative Encryption and Watermarking Algorithm for Vector Maps
Authors: Li Yu , Zhang Liming , Wang Xiaolong , Zhang Xingang , Zhang Qihang
Published: October 2021

Commutative encryption and watermarking (CEW) is an emerging method that combines encryption technology with digital watermarking technology. It has the dual capability of secure transmission and copyright protection. However, the existing CEW methods for vector maps have good robustness in resisting geometric attacks but poor resistance to vertex attacks (e.g., addition, deletion, etc.). To solve this problem, here we propose a novel invariant-based CEW algorithm for vector maps, which consists of permutation-based encryption scheme and coordinates-based watermarking scheme. In the encryption scheme, the encryption key is generated via the Gaussian distribution method combined with the SHA-512 hash method; then, the double random position permutation strategy is applied to the vector map encryption. In watermarking embedding scheme, the original watermark image is scrambled via logistic chaotic encryption before embedding, and the coordinates of all the vertices are normalized. Then, the scrambled watermark image is embedded into the normalized coordinates. Results show that: proposed method is more robust to conventional attacks (e.g., vertex addition and deletion, reordering and data format conversion) and geometric attacks (e.g., scaling and translation). In addition, compared with the existing CEW methods for vector maps, the proposed method has higher security and stronger robustness against vertex attacks.
Structural and Functional Characterization of OXA-48: Insight into Mechanism and Structural Basis of Substrate Recognition and Specificity
Authors: Chiou Jiachi , Cheng Qipeng , Shum Perry Tim-fat , Wong Marcus Ho-yin , Chan Edward Wai-chi , Chen Sheng
Published: October 2021

Class D β-lactamase OXA-48 is widely distributed among Gram-negative bacteria and is an important determinant of resistance to the last-resort carbapenems. Nevertheless, the detailed mechanism by which this β-lactamase hydrolyzes its substrates remains poorly understood. In this study, the complex structures of OXA-48 and various β-lactams were modeled and the potential active site residues that may interact with various β-lactams were identified and characterized to elucidate their roles in OXA-48 substrate recognition. Four residues, namely S70, K73, S118, and K208 were found to be essential for OXA-48 to undergo catalytic hydrolysis of various penicillins and carbapenems both in vivo and in vitro. T209 was found to be important for hydrolysis of imipenem, whereas R250 played a major role in hydrolyzing ampicillin, imipenem, and meropenem most likely by forming a H-bond or salt-bridge between the side chain of these two residues and the carboxylate oxygen ions of the substrates. Analysis of the effect of substitution of alanine in two residues, W105 and L158, revealed their roles in mediating the activity of OXA-48. Our data show that these residues most likely undergo hydrophobic interaction with the R groups and the core structure of the β-lactam ring in penicillins and the carbapenems, respectively. Unlike OXA-58, mass spectrometry suggested a loss of the C6-hydroxyethyl group during hydrolysis of meropenem by OXA-48, which has never been demonstrated in Class D carbapenemases. Findings in this study provide comprehensive knowledge of the mechanism of the substrate recognition and catalysis of OXA-type β-lactamases.
Proteomic Landscape of Adeno-Associated Virus (AAV)-Producing HEK293 Cells
Authors: Strasser Lisa , Boi Stefano , Guapo Felipe , Donohue Nicholas , Barron Niall , Rainbow-Fletcher Alana , Bones Jonathan
Published: October 2021

Adeno-associated viral (AAV) vectors are widely used for gene therapy, providing treatment for diseases caused by absent or defective genes. Despite the success of gene therapy, AAV manufacturing is still challenging, with production yields being limited. With increased patient demand, improvements in host cell productivity through various engineering strategies will be necessary. Here, we study the host cell proteome of AAV5-producing HEK293 cells using reversed phase nano-liquid chromatography and tandem mass spectrometry (RPLC-MS/MS). Relative label-free quantitation (LFQ) was performed, allowing a comparison of transfected vs. untransfected cells. Gene ontology enrichment and pathway analysis revealed differential expression of proteins involved in fundamental cellular processes such as metabolism, proliferation, and cell death. Furthermore, changes in expression of proteins involved in endocytosis and lysosomal degradation were observed. Our data provides highly valuable insights into cellular mechanisms involved during recombinant AAV production by HEK293 cells, thus potentially enabling further improvements of gene therapy product manufacturing.
Phosphonates as Unique Components of Plant Seeds—A Promising Approach to Use Phosphorus Profiles in Plant Chemotaxonomy
Authors: Wieczorek Dorota , Żyszka-Haberecht Beata , Kafka Anna , Lipok Jacek
Published: October 2021

Phosphorus is one of the most important elements essential for all living beings. Plants accumulate and store phosphorous in various forms that have diverse physiological and biochemical functions. In this study, we determine and then examine the phosphorus profiles of seeds of plants belonging to different taxa based on extractable inorganic phosphates and organic forms of phosphorus. We paid particular attention to the presence of natural phosphonates in the tested materials. The inorganic phosphates were determined colorimetrically, whereas phosphorus profiles were created by using 31P NMR spectroscopy. Our study on phosphorus profiles revealed that the obtainedsets of data vary significantly among the representatives of different taxa and were somehow specific for families of plants. It should be emphasised that the measurements obtained using 31P NMR spectroscopy undoubtedly confirmed—for the first time—the presence of phosphonates among the natural components of plant seeds. Hence, the classification of plants considering the phosphorus profiles, including the presence of phosphonates, may be a new additional chemotaxonomic feature.
OCT1 Is a Poor Prognostic Factor for Breast Cancer Patients and Promotes Cell Proliferation via Inducing NCAPH
Authors: Ogura Takuya , Azuma Kotaro , Sato Junichiro , Kinowaki Keiichi , Takayama Ken-Ichi , Takeiwa Toshihiko , Kawabata Hidetaka , Inoue Satoshi
Published: October 2021

Octamer transcription factor 1 (OCT1) is a transcriptional factor reported to be a poor prognostic factor in various cancers. However, the clinical value of OCT1 in breast cancer is not fully understood. In the present study, an immunohistochemical study of OCT1 protein was performed using estrogen receptor (ER)-positive breast cancer tissues from 108 patients. Positive OCT1 immunoreactivity (IR) was associated with the shorter disease-free survival (DFS) of patients (p = 0.019). Knockdown of OCT1 inhibited cell proliferation in MCF-7 breast cancer cells as well as its derivative long-term estrogen-deprived (LTED) cells. On the other hand, the overexpression of OCT1 promoted cell proliferation in MCF-7 cells. Using microarray analysis, we identified the non-structural maintenance of chromosomes condensin I complex subunit H (NCAPH) as a novel OCT1-taget gene in MCF-7 cells. Immunohistochemical analysis showed that NCAPH IR was significantly positively associated with OCT1 IR (p < 0.001) and that positive NCAPH IR was significantly related to the poor DFS rate of patients (p = 0.041). The knockdown of NCAPH inhibited cell proliferation in MCF-7 and LTED cells. These results demonstrate that OCT1 and its target gene NCAPH are poor prognostic factors and potential therapeutic targets for patients with ER-positive breast cancer.
Early Development of Newborn Screening for HCU and Current Challenges
Authors: Levy Harvey L.
Published: October 2021

Classic homocystinuria (HCU) was added to newborn screening (NBS) by Robert Guthrie a few years after the disorder was first described. The justification for NBS was similar to that for PKU, that presymptomatic identification and early dietary treatment would prevent the clinical consequences, which, for HCU, are mental deficiency, ectopia lentis, skeletal abnormalities, and thromboembolism. It was assumed that identifying increased methionine in the screening blood specimen would identify all affected neonates. However, it is now clear that many with HCU are missed by NBS, mainly because the methionine level in the first days of life is normal or below the cutoff level in the NBS program. This includes virtually all of those with B6-responsive HCU. Thus, a more effective method of NBS for HCU should be considered. Included among the possibilities are decreasing the methionine cutoff level, requiring an increase in the Met/Phe ratio if the methionine level is not at or greater than the cutoff level, using methionine as the primary screen with homocysteine as a second-tier test, or replacing methionine with homocysteine as the primary screen. Homocysteine is the primary metabolite that increases in HCU, while the methionine increase is secondary, so homocysteine is usually increased before the increase in methionine, almost always during the first few days of life. Finally, targeted gene screening might be considered. All of these possibilities would impose added expense and labor to NBS, so meeting these challenges would likely require a regional or national effort.