bluuebunny/crossref_metadata_2025 huggingface dataset:
Bacterial diversity and biogeochemistry of different chemosynthetic habitats of the REGAB cold seep (West African margin, 3160 m water depth)
Authors: Pop Ristova P. , Wenzhöfer F. , Ramette A. , Zabel M. , Fischer D. , Kasten S. , Boetius A.
Published: December 2012

Abstract. The giant pockmark REGAB (West African margin, 3160 m water depth) is an active methane-emitting cold seep ecosystem, where the energy derived from microbially mediated oxidation of methane supports high biomass and diversity of chemosynthetic communities. Bare sediments interspersed with heterogeneous chemosynthetic assemblages of mytilid mussels, vesicomyid clams and siboglinid tubeworms form a complex seep ecosystem. To better understand if benthic bacterial communities reflect the patchy distribution of chemosynthetic fauna, all major chemosynthetic habitats at REGAB were investigated using an interdisciplinary approach combining pore water geochemistry, in situ quantification of fluxes and consumption of methane, as well as bacterial community fingerprinting. This study revealed that sediments populated by different fauna assemblages show distinct biogeochemical activities and are associated with distinct sediment bacterial communities. The methane consumption rates and methane effluxes ranged over one to two orders of magnitude across habitats, and reached highest values at the mussel habitat, which hosted a different bacterial community compared to the other habitats. Clam assemblages had a profound impact on the sediment geochemistry, but less so on the bacterial community structure. Moreover, all clam assemblages at REGAB were restricted to sediments characterized by complete methane consumption in the seafloor, and intermediate biogeochemical activity. Overall, variations in the sediment geochemistry were reflected in the distribution of both fauna and microbial communities; and were mostly determined by methane flux.
The relationship between termite mound CH4/CO2 emissions and internal concentration ratios are species specific
Authors: Jamali H. , Livesley S. J. , Hutley L. B. , Fest B. , Arndt S. K.
Published: December 2012

Abstract. 1. We investigated the relative importance of CH4 and CO2 fluxes from soil and termite mounds at four different sites in the tropical savannas of Northern Australia near Darwin and assessed different methods to indirectly predict CH4 fluxes based on CO2 fluxes and internal gas concentrations. 2. The annual flux from termite mounds and surrounding soil was dominated by CO2 with large variations among sites. On a CO2-e basis, annual CH4 flux estimates from termite mounds were 5- to 46-fold smaller than the concurrent annual CO2 flux estimates. Differences between annual soil CO2 and soil CH4 (CO2-e) fluxes were even greater, soil CO2 fluxes being almost three orders of magnitude greater than soil CH4 (CO2-e) fluxes at site. 3. There were significant relationships between mound CH4 flux and mound CO2 flux, enabling the prediction of CH4 flux from measured CO2 flux, however, these relationships were clearly termite species specific. 4. We also observed significant relationships between mound flux and gas concentration inside mound, for both CH4 and CO2, and for all termite species, thereby enabling the prediction of flux from measured mound internal gas concentration. However, these relationships were also termite species specific. Using the relationship between mound internal gas concentration and flux from one species to predict mound fluxes from other termite species (as has been done in past) would result in errors of more than 5-fold for CH4 and 3-fold for CO2. 5. This study highlights that CO2 fluxes from termite mounds are generally more than one order of magnitude greater than CH4 fluxes. There are species-specific relationships between CH4 and CO2 fluxes from a~mound, and between the inside mound concentration of a gas and the mound flux emission of the same gas, but these relationships vary greatly among termite species. Consequently, there is no generic relationship that will allow for the prediction of CH4 fluxes from termite mounds of all species.
Exploring the controls on element ratios in middle Eocene samples of the benthic foraminifera Oridorsalis umbonatus
Authors: Dawber C. F. , Tripati A. K.
Published: December 2012

Abstract. Culturing studies and empirically based core top calibrations have been used to infer that elemental ratios in benthic foraminifera can be used as proxies to reconstruct past variations in bottom water temperature and saturation state (Δ [CO32−]). However the mechanisms linking elemental ratios to these parameters are poorly constrained. Here, we explore the environmental parameters influencing the incorporation of B, Li, Sr and Mg in Oridorsalis umbonatus in early Cenozoic sediments from Ocean Drilling Program Site 1209. We investigate the influence of middle Eocene variations in intermediate water Δ [CO32−] using relationships developed from core top samples. The fidelity of bottom water Δ[CO32−] reconstructions based on single element ratios is assessed by comparing the X/Ca-based reconstructions to each other and to carbon cycle proxy records (benthic foraminifera δ13C, organic carbon content, foraminifera dissolution indices), and a seawater δ18O reconstruction for Site 1209. Discrepancies in the reconstructed Δ[CO32−] values based on these different metal ratios suggest that there are still gaps in our understanding of the parameters influencing X/Ca and demonstrate that caution is required when interpreting palaeo-reconstructions that are derived from a single elemental ratio. The downcore record of O. umbonatus Mg/Ca does not exhibit any similarities with the Li/Ca, B/Ca and Sr/Ca records, suggesting that the environmental parameters influencing Mg/Ca may be different for this species, consistent with temperature as the strongest control on this elemental ratio. This hypothesis is supported by the coefficients of multiple linear regression models on published Mg/Ca data. An incomplete understanding of the controls on elemental incorporation into benthic foraminifera hinders our ability to confidently quantify changes in saturation state using single X/Ca reconstructions over a range of timescales.
Pollen-based reconstruction of Holocene vegetation and climate in southern Italy: the case of Lago Trifoglietti
Authors: Joannin S. , Brugiapaglia E. , de Beaulieu J.-L. , Bernardo L. , Magny M. , Peyron O. , Goring S. , Vannière B.
Published: December 2012

Abstract. A high-resolution pollen record from Lago Trifoglietti in Calabria (southern Italy) provides new insights into the paleoenvironmental and palaeoclimatic changes which characterise the Holocene period in the southern Italy. The chronology is based on 11 AMS radiocarbon dates from terrestrial organic material. The Holocene history of the vegetation cover shows the persistence of an important and relatively stable Fagus forest present over that entire period, offering a rare example of a beech woodstand able to withstand climate changes for more than 11 000 yr. Probably in relation with early Holocene dry climate conditions which affected southern Italy, the Trifoglietti pollen record supports a southward delay in thermophyllous forest expansion dated to ca. 13 500 cal BP at Monticchio, ca. 11 000 cal BP at Trifoglietti, and finally ca. 9800 cal BP in Sicily. Regarding the human impact history, the Trifoglietti pollen record shows only poor imprints of agricultural activities and anthopogenic indicators, apart from those indicating pastoralism activities beneath forest cover. The selective exploitation of Abies appears to have been the strongest human impact on the Trifoglietti surroundings. On the basis of (1) a specific ratio between hygrophilous and terrestrial taxa, and (2) the Modern Analogue Technique, the pollen data collected at Lago Trifoglietti led to the establishment of two palaeoclimatic records tracing changes in (1) lake depth and (2) annual precipitation. On a millennial scale, these records give evidence of increasing moisture from ca. 11 000 to ca. 9400 cal BP and maximum humidity from ca. 9400 to ca. 6200 cal BP, prior to a general trend towards the drier climate conditions that have prevailed up to the present. In addition, several successive centennial-scale oscillations appear to have punctuated the entire Holocene. The identification of a cold dry event around 11 300 cal BP, responsible for a marked decline in timberline altitude and possibly equivalent to the PBO, remains to be confirmed by further investigations verifying both chronology and magnitude. Two cold and possibly drier Boreal oscillations developed at ca. 9800 and 9200 cal BP. At Trifoglietti, the 8.2 kyr event corresponds to the onset of cooler and drier climatic conditions which persisted until ca. 7500 cal BP. Finally, the second half of the Holocene was characterised by dry phases at ca. 6100–5200, 4400–3500, and 2500–1800 cal BP, alternating with more humid phases at ca. 5200–4400 and ca. 3500–2500 cal BP. Considered as a whole, these millennial-scale trends and centennial-scale climatic oscillations support contrasting patterns of palaeohydrological changes recognised between the north- and south-central Mediterranean.
Low-cost multi-stage filtration enhanced by coagulation-flocculation in upflow gravel filtration
Authors: Sánchez L. D. , Marin L. M. , Visscher J. T. , Rietveld L. C.
Published: December 2012

Abstract. This paper assesses the operational and design aspects of coagulation and flocculation in upflow gravel filters (CF-UGF) in a multi-stage filtration (MSF) plant. This study shows that CF-UGF units improve the performance of MSF considerably, when the system operates with turbidity above 30 NTU. It strongly reduces the load of particulate material before the water enters in the slow sand filters (SSF) and therewith avoids short filter runs and prevents early interruption in SSF operations. The removal efficiency of turbidity in the CF-UGF with coagulant was between 85 and 96%, whereas the average efficiency without coagulant dosing was 46% (range: 21–76%). Operating with coagulant also improves the removal efficiency for total coliforms, E-coli and HPC. No reduction was observed in the microbial activity of the SSF, no obstruction of the SSF bed was demonstrated and SSF runs were maintained between 50 and 70 days for a maximum head loss of 0.70 m. The most important advantage is the flexibility of the system to operate with and without coagulant according to the influent turbidity. It was only necessary for 20% of the time to operate with the coagulant. The CF-UGF unit represented 7% of total construction costs and the O&M cost for the use of coagulant represented only 0.3%.
What drives flood trends along the Rhine River: climate or river training?
Authors: Vorogushyn S. , Merz B.
Published: December 2012

Abstract. The Rhine River catchment was heavily trained over the past decades and faced the construction of the Rhine weir cascade, flood protection dikes and detention basins. For the same time period, several studies detected positive trends in flood flows and faced the challenge of flood trend attribution, i.e. identifying the drivers of observed change. The presented study addresses the question about the responsible drivers for changes in annual maximum daily flows at Rhine gauges starting from Maxau down to Lobith. In particular, the role of river training measures including the Rhine weir cascade and a series of detention basins in enhancing Rhine floods was investigated. By applying homogenisation relationships to the original flow records in the period from 1952 till 2009, the annual maximum series were computed that would have been recorded had river training measures not been in place. Using multiple trend analysis, the relative changes in the homogenised time series were found to be smaller up to about 20% points compared to the original records. This effect is attributable to the river training measures and primarily to the construction of the Rhine weir cascade. The increase in Rhine flood discharges was partly caused by the unfavourable superposition of the Rhine and Neckar flood waves. It resulted from the acceleration of the Rhine waves due to construction of the weir cascade. However, at the same time, the tributary flows across the entire Upper and Lower Rhine, which enhance annual Rhine peaks, showed very strong positive trends. This suggests the dominance of a large-scale driver such as climate variability/change which acted along with river training. In particular, the analysis suggests that the river training measures fell in a period with increasing flood trends driven by factors other than river training of the Rhine main channel.
Propagation of Atlantic Ocean swells in the north Indian Ocean: a case study
Authors: Samiksha S. V. , Vethamony P. , Aboobacker V. M. , Rashmi R.
Published: December 2012

Abstract. An analysis of altimeter significant wave height data of May 2007 revealed the occurrence of an extreme weather event off southern tip of South Africa in the Atlantic Ocean, and generation of a series of very high swells at 40° S. These swells propagated towards northeast and broke over La Réunion island in the Indian Ocean on 12 May 2007. The wave model WAVEWATCH III was used to study the propagation of these swells in the Indian Ocean. The model was validated for the Indian Ocean using moored buoy data at 12 locations and merged altimeter wave data. The wave model accurately reproduced the event of May 2007. Swell heights, of the order of 15.0 m, at the generation area reduced to 6.0 m near La Réunion island. This study shows that the swells generated in the Roaring Forties of the Atlantic Ocean (between 15° to 80° E longitude) propagate in the NE/NNE direction towards the north Indian Ocean, and wave characteristics of the Arabian Sea are least influenced compared to that of Bay of Bengal, when swells from the Atlantic Ocean enter the Indian Ocean. The double peak spectrum extracted for the Bay of Bengal indicates that one of the peaks is due to swells generated off southern tip of South Africa.
Mass balance, runoff and surges of the Bering Glacier, Alaska
Authors: Tangborn W.
Published: December 2012

Abstract. The historical net, ablation and accumulation daily balances and runoff of the Bering Glacier, Alaska are determined for the 1951–2011 period with the PTAA (precipitation-temperature-area-altitude) model, using daily precipitation and temperature observations collected at the Cordova and Yakutat weather stations, together with the area-altitude distribution of the glacier. The mean annual balance for this 61-yr period is −0.6 mwe, the accumulation balance is +1.4 and the ablation balance is −2.0 mwe. Periodic surges of this glacier transport large volumes of ice to lower elevations where the ablation rate is higher, producing more negative balances and increasing runoff. During the 1993–1995 surge the average ablation balance is −3.3 mwe, over a meter greater than the 1951–2011 average. Runoff from the Bering Glacier (derived from simulated ablation and precipitation as rain) is highly correlated with the four glacier surges that have been observed since 1951. Ice volume loss for the 1972–2003 period measured with the PTAA model is 2.3 km3 we a−1 and closely agrees with losses for the same period measured with the geodetic method.
Echiodon prionodon, a new species of Carapidae (Pisces, Ophidiiformes) from New Zealand
Authors: Parmentier Eric
Published: December 2012

A new species of pearlfish, Echiodon prionodon, is described from three specimens. This species is diagnosed by having a serrated margin on the posterior edge of the fangs, expanded thoracic plates on some abdominal vertebrae and ventral swimbladder tunic ridges. This species was only found in coastal waters around the North Island of New Zealand. The diagnosis of Eurypleuron is revised.
A Core–Shell Albumin Copolymer Nanotransporter for High Capacity Loading and Two‐Step Release of Doxorubicin with Enhanced Anti‐Leukemia Activity
Authors: Wu Yuzhou , Ihme Susann , Feuring‐Buske Michaela , Kuan Seah Ling , Eisele Klaus , Lamla Markus , Wang Yanran , Buske Christian , Weil Tanja
Published: December 2012

The native transportation protein serum albumin represents an attractive nano‐sized transporter for drug delivery applications due to its beneficial safety profile. Existing albumin‐based drug delivery systems are often limited by their low drug loading capacity as well as noticeable drug leakage into the blood circulation. Therefore, a unique albumin‐derived core‐shell doxorubicin (DOX) delivery system based on the protein denaturing‐backfolding strategy was developed. 28 DOX molecules were covalently conjugated to the albumin polypeptide backbone via an acid sensitive hydrazone linker. Polycationic and pegylated human serum albumin formed two non‐toxic and enzymatically degradable protection shells around the encapsulated DOX molecules. This core‐shell delivery system possesses notable advantages, including a high drug loading capacity critical for low administration doses, a two‐step drug release mechanism based on pH and the presence of proteases, an attractive biocompatibility and narrow size distribution inherited from the albumin backbone, as well as fast cellular uptake and masking of epitopes due to a high degree of pegylation. The IC50 of these nanoscopic onion‐type micelles was found in the low nanomolar range for Hela cells as well as leukemia cell lines. In vivo data indicate its attractive potential as anti‐leukemia treatment suggesting its promising profile as nanomedicine drug delivery system.
Mobility, mortality, and the middle ages: Identification of migrant individuals in a 14th century black death cemetery population
Authors: Kendall E.J. , Montgomery J. , Evans J.A. , Stantis C. , Mueller V.
Published: December 2012

Mobility and migration patterns of groups and individuals have long been a topic of interest to archaeologists, used for broad explanatory models of cultural change as well as illustrations of historical particularism. The 14th century AD was a tumultuous period of history in Britain, with severely erratic weather patterns, the Great Famine of 1315–1322, the Scottish Wars of Independence, and the Hundred Years' War providing additional migration pressures to the ordinary economic issues drawing individuals to their capital under more stable conditions. East Smithfield Black Death Cemetery (Royal Mint) had a documented use period of only 2 years (AD 1348–1350), providing a precise historical context (∼50 years) for data. Adults (n = 30) from the East Smithfield site were sampled for strontium and oxygen stable isotope analyses of tooth enamel. Five individuals were demonstrated to be statistical outliers through the combined strontium and oxygen isotope data. Potential origins for migrants ranged from London's surrounding hinterlands to distant portions of northern and western Britain. Historic food sourcing practices for London were found to be an important factor for consideration in a broader than expected 87Sr/86Sr range reflected in a comparison of enamel samples from three London datasets. The pooled dataset demonstrated a high level of consistency between site data, divergent from the geologically predicted range. We argue that this supports the premise that isotope data in human populations must be approached as a complex interaction between behavior and environment and thus should be interpreted cautiously with the aid of alternate lines of evidence. Am J Phys Anthropol, 2013. © 2012 Wiley Periodicals, Inc.
Morphological variation of genetically confirmedAlouatta Pigra×A. palliatahybrids from a natural hybrid zone in Tabasco, Mexico
Authors: Kelaita Mary A. , Cortés‐Ortiz Liliana
Published: December 2012

While hybridization has been reported for a large number of primate taxa, there is a general lack of data on hybrid morphology for wild individuals with known genetic ancestry. A confirmed hybrid zone for the closely related Neotropical primatesAlouatta palliataandA. pigrahas provided a unique opportunity to study primate hybrid morphological variation. Here we used molecular evidence based on mitochondrial, Y‐chromosome, and autosomal data to assess hybrid ancestry. We conducted univariate and multivariate statistical comparisons of morphometric data collected from individuals both outside and within the hybrid zone in Tabasco, Mexico. Our results show that of all the hybrids detected (N= 128), only 12% of them were approximately genetically intermediate, and none of them were first generation hybrids. Univariate pairwise comparisons among parental individuals, multigenerational backcrossed hybrids, and intermediate hybrids showed that overall, multigenerational backcrossed hybrids resemble the parental species with which they share most of their alleles. Conversely, intermediates were highly variable. Similarly, principal component analysis depicts an overlap between the parental species and their backcrosses when considering overall morphological differences. Finally, discriminant function analysis of the morphological variables was overall unreliable for classifying individuals into their assigned genotypic classes. Taken together, our results suggest that primate natural hybridization studies should incorporate molecular methods for determining ancestry, because morphology may not always be a reliable indicator of hybrid status. Hybrid zones could comprise a large number of multigenerational backcrossed hybrids that are indistinguishable from the parental species. The implications for studying hybridization in the primate fossil record are discussed. Am J Phys Anthropol, 2013. © 2012 Wiley Periodicals, Inc.
Consensus Of Identical Linear Systems with Communication Delays by Using the Information of Second‐Order Neighbors
Authors: Rong Lina , Xu Shengyuan , Zhang Baoyong , Yuan Deming
Published: December 2012

This paper studies the consensus protocol using the information of second‐order neighbors in undirected and connected networks of linear systems with communication delays. The feedback matrix which guarantees consensus under both the proposed and the traditional protocol is given; it is shown that with this matrix, the networks under the proposed protocol converge faster than the traditional protocol. Based on this result, the delay sensitivity of the proposed protocol is considered under an assumption about the communication topology. The maximum allowable upper bound of the delays is obtained by solving certain linear matrix inequalities. Two simulation examples are presented to illustrate the effectiveness of the theoretical results.
A diclofenac suppository–nabumetone combination therapy for arthritic pain relief and a monitoring method for the diclofenac binding capacity of HSA site II in rheumatoid arthritis
Authors: Setoguchi Nao , Takamura Norito , Fujita Ken‐ichi , Ogata Kenji , Tokunaga Jin , Nishio Toyotaka , Chosa Etsuo , Arimori Kazuhiko , Kawai Keiichi , Yamamoto Ryuichi
Published: December 2012

Diclofenac suppository, a non‐steroidal anti‐inflammatory drug (NSAID), is used widely in rheumatoid arthritis (RA) patients with severe arthritic pain. As the binding percentage of diclofenac to serum proteins is high, its free (unbound) concentration after rectal administration is low. To increase temporarily the free concentration of diclofenac and to enhance its analgesic effect by inhibiting the protein binding of diclofenac, the analgesic effect of diclofenac was examined before and after the start of an inhibitor administration to RA patients with insufficient control of arthritic pain, and the protein binding capacity of diclofenac was evaluated. Binding experiments were performed by ultrafiltration, and arthritic pain was recorded by the face scale. Free fractions of diazepam and diclofenac were augmented by increasing 6‐methoxy‐2‐naphthylacetic acid (6‐MNA; the active metabolite of the NSAID nabumetone) concentrations. The free fraction of diazepam increased after the start of nabumetone administration to RA patients, and arthritic pain relief was observed. These results suggest that 6‐MNA has an inhibitory effect on the protein binding of diclofenac and the free fraction of diazepam can be used to evaluate the binding capacity of diclofenac. It is considered that diclofenac suppository–nabumetone combination therapy and the method for protein binding monitoring by diazepam can positively benefit RA patients with insufficient control of arthritic pain. Copyright © 2013 John Wiley & Sons, Ltd.
Outcome of surgical resection of second-time locally recurrent rectal cancer
Authors: Harji D P , Sagar P M , Boyle K , Maslekar S , Griffiths B , McArthur D R
Published: December 2012

Background

Locally recurrent rectal cancer relapses in the pelvis in up to 60 per cent of patients following resection. This study assessed the surgical and oncological outcomes of patients who underwent surgery for re-recurrent rectal cancer.

Methods

Patients who underwent second-time resection of locally recurrent rectal cancer between 2001 and 2010 were eligible for inclusion. Data were collected on demographics, presentation of disease, preoperative staging imaging, adjuvant therapy, operative detail, histopathology and follow-up status (clinical and imaging) for the primary tumour, and first and second recurrences.

Results

Thirty patients (of 56 discussed at the multidisciplinary meeting) underwent resection of re-recurrent rectal cancer. Postoperative morbidity occurred in nine patients but none died within 30 days. Negative resection margins (R0) were achieved in ten patients, microscopic margin positivity (R1) was evident in 15 and macroscopic involvement (R2) was found in five. Although no patient had distant metastatic disease, 22 had involvement of the pelvic side wall. One- and 3-year overall survival rates were 77 and 27 per cent respectively, with a median overall survival of 23 (range 3–78) months. An R0 resection conferred a survival benefit (median survival 32 (11–78) months versus 19 (6–33) months after R1 and 7 (3–10) months after R2 resection).

Conclusion

Surgical resection of re-recurrent rectal cancer had comparable surgical and oncological outcomes to initial recurrences in well selected patients.
Ultra‐high pressure LC for astaxanthin determination in shrimp by‐products and active food packaging
Authors: Sanches‐Silva A. , Ribeiro T. , Albuquerque T. G. , Paseiro P. , Sendón R. , de Quirós A. Bernaldo , López‐Cervantes J. , Sánchez‐Machado D. I. , Soto Valdez H. , Angulo I. , Aurrekoetxea G. P. , Costa H. S.
Published: December 2012

Nowadays, there is increasing interest in natural antioxidants from food by‐products. Astaxanthin is a potent antioxidant and one of the major carotenoids in crustaceans and salmonids. An ultra‐high pressure liquid chromatographic method was developed and validated for the determination of astaxanthin in shrimp by‐products, and its migration from new packaging materials to food simulants was also studied. The method uses an UPLC® BEH guard‐column (2.1 × 5 mm, 1.7 µm particle size) and an UPLC® BEH analytical column (2.1 × 50 mm, 1.7 µm particle size). Chromatographic separation was achieved using a programmed gradient mobile phase consisting of (A) acetonitrile–methanol (containing 0.05 m ammonium acetate)–dichloromethane (75:20:5, v/v/v) and (B) ultrapure water. This method was evaluated with respect to validation parameters such as linearity, precision, limit of detection, limit of quantification and recovery. Low‐density polyethylene films were prepared with different amounts of the lipid fraction of fermented shrimp waste by extrusion, and migration was evaluated into food simulants (isooctane and ethanol 95%, v/v). Migration was not detected under the tested conditions. Copyright © 2012 John Wiley & Sons, Ltd.
Climate Change Response: Evidence from the Margaret River Wine Region of Australia
Authors: Galbreath Jeremy
Published: December 2012

This study explores the relevancy of climate change to business using a sample of wine firms operating in Margaret River, Western Australia, one of the premier wine regions of the world. Using a qualitative approach based on thematic analysis, the results challenge the extent to which climate change is a salient stakeholder, while demonstrating that the phenomenon may, in fact, be beneficial. Response actions towards climate change demonstrate both mitigative and adaptive actions, although differences in their level and rate of implementation appear to be attributable to a mix of normative and instrumental trade‐offs. Implications of the findings are discussed, with a particular focus on location theory and economic barriers as a key driver of trade‐offs between the choice of mitigative or adaptive response to climate change. Copyright © 2012 John Wiley & Sons, Ltd and ERP Environment.
Fluid flow in a spiral device used for irradiation of biological fluids
Authors: Nikolof Todd , Prakash Mahesh , Cleary Paul W , Bertolini Joseph
Published: December 2012

The manufacture of plasma‐derived therapeutics includes dedicated viral inactivation steps to minimize the risk of infection. Traditional viral inactivation methods are effective for the removal and inactivation of enveloped viruses, but less effective against small nonenveloped viruses. UV‐C irradiation has been demonstrated to be an effective means of inactivating such viruses. The UVivatec lab system consists of a spiral tube around an UV‐C irradiation source. Flow of a solution through the chamber generates and ensures controlled mixing and uniform exposure to irradiation. A detailed assessment of the effect of flow rate, alternate cross sectional design and scale up of the irradiation chamber on Dean vortices was performed using the smoothed particle hydrodynamics method. The aim was to provide a basis for setting flow rate limits and using a laboratory scale apparatus to model viral inactivation in larger manufacturing scale equipment. The effect of flow rate related changes on the fluence rate was also investigated through chemical actinometry studies. The data were consistent with the simulations indicating that Dean vortices were present at low flow rates, but dissipated at higher flow rates through the spiral chamber. Importantly, this work also allowed a correlation between the small system and large scale system to be established. This will greatly facilitate process development and viral validation studies. © 2013 American Institute of Chemical Engineers Biotechnol. Prog., 29: 359–367, 2013
A segment‐based sparse matrix–vector multiplication on CUDA
Authors: Feng Xiaowen , Jin Hai , Zheng Ran , Shao Zhiyuan , Zhu Lei
Published: December 2012

The challenge for Sparse Matrix–Vector multiplication (SpMV) performance is memory bandwidth, which mostly depends on input matrices and underlying computing platforms. To solve this challenge, many researchers have explored a variety of optimization techniques. One of the most promising aspects focuses on designing storage formats to represent sparse matrices. However, lots of prior storage formats cannot fully take advantage of the underlying computing platforms, resulting in unsatisfactory performance and large memory footprint. Therefore, a novel storage format, called Segmented Hybrid ELL + Compressed Sparse Row (CSR) (SHEC for short), is proposed to further improve the throughput and lessen memory footprint on Graphics Processing Unit (GPU). SHEC format employs an interleaved combination pattern, which combines certain amount of compressed rows to form a new SHEC row. Segmentation is brought in to balance load and reduce memory footprint. According to the empirical data, an automatic SHEC‐based SpMV is developed to fit for all the matrices. Experimental results show that SHEC approach outperforms the best results of NVIDIA SpMV library and exhibits a comparable performance with state‐of‐the‐art storage formats on the standard dataset. Copyright © 2012 John Wiley & Sons, Ltd.
Finite volume simulation of unsteady shock‐cavitation in compressible water
Authors: Causon D. M. , Mingham C. G.
Published: December 2012

The present paper extends an established Riemann‐based finite volume hydrocode for compressible pure water to admit change of phase. The thermal and caloric behaviour of water and water vapour is described by a one‐fluid model on the assumption that the two‐phase regime can be described locally within a finite volume cell as a homogeneous mixture that remains in thermodynamic equilibrium. Closure of the equation set is achieved by deriving equations of state for pure fluids and the mixture that cover all possible fluid states. Following a description of the flow model and the numerical method, computations are carried out to demonstrate the potential of the method. Calculations include a one‐dimensional cavitation tube, one‐dimensional condensation front, collapse of a cavitation bubble in water in one dimension, and an unsteady two‐dimensional hypervelocity flow past a cavitating hydrofoil. Copyright © 2012 John Wiley & Sons, Ltd.
In Situ Soft X‐ray Microscopy Study of Fe Interconnect Corrosion in Ionic Liquid‐Based Nano‐PEMFC Half‐Cells
Authors: Bozzini B. , Gianoncelli A. , Kaulich B. , Mele C. , Prasciolu M. , Kiskinova M.
Published: December 2012

This in situ soft X‐ray scanning microscopy electrochemical study of model proton exchange cathodic and anodic nano‐fuel cells is exploring the evolving structure and chemical composition of key cell components represented by Au and Fe electrodes in contact with Nafion‐ionic liquid composite electrolyte containing Pt black catalyst particles. Morphological and chemical changes of the electrodes as well as the chemical state and fate of the Fe species released into the electrolyte are monitored in short circuit and with applied cathodic or anodic polarization. The in situ X‐ray absorption images of the cathodic cell fed with 2.5 × 10–5 mbar O2 have revealed corrosion‐induced morphology changes in the Fe electrode, being more pronounced in the vicinity of Pt‐black particles, and deposition of the Fe species released into the electrolyte, onto the intact Au counter electrode upon cathodic polarization. The Fe electrodes of the anodic cell containing NaBH4 in the electrolyte appear relatively more corrosion resistant. The Fe L3 absorption spectra taken in different locations within the Fe electrode have shown lateral variations in the relative ratio between Fe2+ and Fe3&4+ oxidation states, whereas the Fe species released into the RTIL electrolyte are only in the high Fe3&4+ oxidation states.
Late‐life depression in older African Americans: a comprehensive review of epidemiological and clinical data
Authors: Pickett Yolonda R. , Bazelais Kisha N. , Bruce Martha L.
Published: December 2012

Objective

The population of older African Americans is expected to triple by 2050, highlighting the public health importance of understanding their mental health needs. Despite evidence of the negative impact of late‐life depression, less is known of how this disorder affects the lives of older African Americans. Lack of studies focusing on how depression presents in older African Americans and their subsequent treatment needs lead to a gap in epidemiologic and clinical knowledge for this population. In this review, we aim to present a concise report of prevalence, correlates, course, outcomes, symptom recognition, and treatment of depression for these individuals.

Method

We performed a literature review of English‐language articles identified from PubMed and Medline published between January 1990 and June 2012. Studies included older adults and contained the key words ‘geriatric depression in African Americans’, ‘geriatric depression in Blacks’, and ‘geriatric depression in minorities’.

Results

Although in most studies, older African Americans had higher or equivalence prevalence of depression compared with Caucasian Americans, we also found lower rates of recognition of depression and treatment. Many studies reported worse outcomes associated for depression among older African Americans compared with older Caucasians.

Conclusions

Serious racial and ethnic disparities persist in the management of older African Americans with depression. Understanding their unmet needs and improving depression care for these individuals is necessary to reduce these disparities. Copyright © 2012 John Wiley & Sons, Ltd.
Response of hydrology to climate change in the southern Appalachian Mountains using Bayesian inference
Authors: Wu Wei , Clark James S. , Vose James M.
Published: December 2012

Predicting long‐term consequences of climate change on hydrologic processes has been limited due to the needs to accommodate the uncertainties in hydrological measurements for calibration, and to account for the uncertainties in the models that would ingest those calibrations and uncertainties in climate predictions as basis for hydrological predictions. We implemented a hierarchical Bayesian (HB) analysis to coherently admit multiple data sources and uncertainties including data inputs, parameters, and model structures to identify the potential consequences of climate change on soil moisture and streamflow at the head watersheds ranging from low to high elevations in the southern Appalachian region of the United States. We have considered climate change scenarios based on three greenhouse gas emission scenarios of the Interovernmental Panel on Climate Change: A2, A1B, and B1 emission scenarios.

Full predictive distributions based on HB models are capable of providing rich information and facilitating the summarization of prediction uncertainties. With predictive uncertainties taken into account, the most pronounced change in soil moisture and streamflow would occur under the A2 scenario at both low and high elevations, followed by the A1B scenario and then by the B1 scenario. Uncertainty in the change of soil moisture is less than that of streamflow for each season, especially at high elevations. A reduction of soil moisture in summer and fall, a reduction or slight increase of streamflow in summer, and an increase of streamflow in winter are predicted for all three scenarios at both low and high elevations.

The hydrological predictions with quantified uncertainties from a HB model could aid more‐informed water resource management in developing mitigation plans and dealing with water security under climate change. Copyright © 2012 John Wiley & Sons, Ltd.
FINANCIAL INTEGRATION AND EXTERNAL SUSTAINABILITY
Authors: Towbin Pascal
Published: December 2012

A stable net external position requires that the trade balance responds negatively to changes in the net external position. If financial integration makes financing external imbalances less costly, we expect slower external adjustment in more integrated economies. The study estimates theoretically founded trade balance reaction functions for a panel of 70 countries from 1970–2008. The empirical analysis finds that adjustment in integrated economies is slower. Consistent with the presented theory, the trade balance of integrated economies is more persistent, responds less strongly to net foreign assets and is more sensitive to fluctuations in net output. Under high integration, the response to the net external position is weak and close to the minimum required to ensure external sustainability. Copyright © 2012 John Wiley & Sons, Ltd.
Systematic investigation of protein–small molecule interactions
Authors: Li Xiyan , Wang Xin , Snyder Michael
Published: December 2012

Cell signaling is extensively wired between cellular components to sustain cell proliferation, differentiation, and adaptation. The interaction network is often manifested in how protein function is regulated through interacting with other cellular components including small molecule metabolites. While many biochemical interactions have been established as reactions between protein enzymes and their substrates and products, much less is known at the system level about how small metabolites regulate protein functions through allosteric binding. In the past decade, study of protein–small molecule interactions has been lagging behind other types of interactions. Recent technological advances have explored several high‐throughput platforms to reveal many “unexpected” protein–small molecule interactions that could have profound impact on our understanding of cell signaling. These interactions will help bridge gaps in existing regulatory loops of cell signaling and serve as new targets for medical intervention. In this review, we summarize recent advances of systematic investigation of protein–metabolite/small molecule interactions, and discuss the impact of such studies and their potential impact on both biological researches and medicine. © 2012 IUBMB Life, 65(1):2–8, 2013
Rapid immuno‐SERS microscopy for tissue imaging with single‐nanoparticle sensitivity
Authors: Salehi Mohammad , Steinigeweg Dennis , Ströbel Philipp , Marx Alexander , Packeisen Jens , Schlücker Sebastian
Published: December 2012

Immuno‐SERS microscopy is a novel imaging technique in nano‐biophotonics, which employs antibodies labeled with SERS‐active nanoparticles in conjunction with Raman microscopy. Rapid data acquisition is of central importance for screening large areas of tissue specimens. Here, we first discuss the role of SERS labels with single‐particle sensitivity in immuno‐SERS microscopy, in particular with respect to false‐negative results. In combined single‐particle experiments (SERS microscopy/dark‐field microscopy/HR‐SEM), we then demonstrate that small glass‐coated clusters (dimers and trimers) of gold nanospheres exhibit the desired single‐particle SERS sensitivity, even at acquisition times as short as 30 msec per pixel, while monomers do not. The proof‐of‐concept for rapid immuno‐SERS microscopy with 30 msec acquisition time per pixel for selective imaging of the p53 family member p63 in prostate tissue sections is demonstrated. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Efficient implementation of restricted active space configuration interaction with the hole and particle approximation
Authors: Casanova David
Published: December 2012

The restricted active space configuration interaction (RASCI) formalism with the hole and particle truncation of the wavefunction, that is, RASCI(h,p), holds very nice properties such as balanced treatment of ground and low‐lying excited states, spin‐completeness, large flexibility of the wavefunction, and moderate computational cost. In this article, I present a new implementation of the RASCI(h,p) method using a general algorithm based on the integral‐driven approach. The new implementation allows to choose any electronic configuration as the single reference in combination with an excitation operator with any number of ionization, electron attachment, or spin‐flip (SF) excitations. The applicability and good performance of the new computational code is tested in the ground state calculation of water molecule with increasingly large active spaces and up to the full‐CI limit, the calculation of all‐trans linear polyenes with variable number of SF excitations, and the low‐lying states of fluorine molecule with a double‐ionization potential operator. © 2012 Wiley Periodicals, Inc.
Membrane protein native state discrimination by implicit membrane models
Authors: Yuzlenko Olga , Lazaridis Themis
Published: December 2012

Four implicit membrane models [IMM1, generalized Born (GB)‐surface area‐implicit membrane (GBSAIM), GB with a simple switching (GBSW), and heterogeneous dielectric GB (HDGB)] were tested for their ability to discriminate the native conformation of five membrane proteins from 450 decoys generated by the Rosetta‐Membrane program. The energy ranking of the native state and Z‐scores were used to assess the performance of the models. The effect of membrane thickness was examined and was found to be substantial. Quite satisfactory discrimination was achieved with the all‐atom IMM1 and GBSW models at 25.4 Å thickness and with the HDGB model at 28.5 Å thickness. The energy components by themselves were not discriminative. Both van der Waals and electrostatic interactions contributed to native state discrimination, to a different extent in each model. Computational efficiency of the models decreased in the order: extended‐atom IMM1 > all‐atom IMM1 > GBSAIM > GBSW > HDGB. These results encourage the further development and use of implicit membrane models for membrane protein structure prediction. © 2012 Wiley Periodicals, Inc.
Exploring the dark side of organizational citizenship behavior
Authors: Bolino Mark C. , Klotz Anthony C. , Turnley William H. , Harvey Jaron
Published: December 2012

In recent years, there has been increasing interest in positive organizational scholarship in general, including positive organizational behavior (POB) in particular. This work identifies organizational citizenship behavior (OCB) as a prototypical POB. Conceptualizing OCBs in this way is sensible in light of more than 30 years of research highlighting the desirable aspects of such behavior. At the same time, some researchers have raised questions about positive organizational scholarship and have called for a more balanced view of ostensibly positive behaviors. The purpose of this paper, then, is to take a more nuanced view of OCBs while highlighting the dark side of citizenship behavior. In doing so, we review conceptual and empirical work that has challenged the idea that OCBs are inherently positive. We also discuss research that seeks to develop a deeper understanding of the conditions under which OCB does more harm than good. Finally, important areas for future research and the practical realities facing scholars who seek to publish research investigating the dark side of citizenship are addressed as well. Copyright © 2012 John Wiley & Sons, Ltd.
The evolution of traumatic brain injury in a rat focal contusion model
Authors: Turtzo L. Christine , Budde Matthew D. , Gold Eric M. , Lewis Bobbi K. , Janes Lindsay , Yarnell Angela , Grunberg Neil E. , Watson William , Frank Joseph A.
Published: December 2012

Serial MRI facilitates the in vivo analysis of the intra‐ and intersubject evolution of traumatic brain injury lesions. Despite the availability of MRI, the natural history of experimental focal contusion lesions in the controlled cortical impact (CCI) rat model has not been well described. We performed CCI on rats and MRI during the acute to chronic stages of cerebral injury to investigate the time course of changes in the brain. Female Wistar rats underwent CCI of their left motor cortex with a flat impact tip driven by an electromagnetic piston. In vivo MRI was performed at 7 T serially over 6 weeks post‐CCI. The appearances of CCI‐induced lesions and lesion‐associated cortical volumes were variable on MRI, with the percentage change in cortical volume of the CCI ipsilateral side relative to the contralateral side ranging from 18% within 2 h of injury on day 0 to a peak of 35% on day 1, and a trough of –28% by week 5/6, with an average standard deviation of ±14% at any given time point. In contrast, the percentage change in cortical volume of the ipsilateral side relative to the contralateral side in control rats was not significant (1 ± 2%). Hemorrhagic conversion within and surrounding the CCI lesion occurred between days 2 and 9 in 45% of rats, with no hemorrhage noted on the initial scan. Furthermore, hemorrhage and hemosiderin within the lesion were positive for Prussian blue and highly autofluorescent on histological examination. Although some variation in injuries may be technique related, the divergence of similar lesions between initial and final scans demonstrates the inherent biological variability of the CCI rat model. Published 2012. This article is a US Government work and is in the public domain in the USA.