bluuebunny/crossref_metadata_2025 huggingface dataset:
NATURAL "FLOW" NOT IN LE JAN–RAIMOND FRAMEWORK
Authors: CHEN JUN , XIANG KAI-NAN
Published: May 2011

A natural "flow" over super-Brownian motions, which is not in Le Jan–Raimond framework on stochastic flows of measurable maps and kernels, is discovered in this paper.
Ice-marginal and periglacial processes and sediments: an introduction
Authors: Martini I. Peter , French Hugh M. , Alberti Augusto Pérez
Published: May 2011

The volume focuses on the analysis of glacial clastic sedimentary deposits, both ancient and recent. The papers range from reviews of glacial systems and cold-climate weathering products and processes to conceptual and field studies of specific ice-marginal and cold-climate sediments. Papers are included that deal with tidewater glaciers, mountain settings on Earth, permafrost areas on both Earth and Mars and detailed regional analyses of cold-climate sediments of Late Pleistocene and Holocene age. The identification of sedimentary facies allows an accurate reconstruction of many of the developmental processes that are involved in ice-marginal and periglacial environments. Lithostratigraphic characteristics of clastic deposits also constitute circumstantial evidence for the previous existence of ancient, and certainly pre-Quaternary, cold-climate systems. This is demonstrated by a study on putative Palaeozoic glacial deposits in Saudi Arabia.
Gullies, polygons and mantles in Martian permafrost environments: cold desert landforms and sedimentary processes during recent Martian geological history
Authors: Levy J. S. , Head J. W. , Marchant D. R.
Published: May 2011

A range of cold desert landforms are found on the Martian surface that have been interpreted to indicate prevailing frozen and hyper-arid conditions for at least the past several million years. These cold desert conditions are punctuated by brief periods of localized surficial liquid water flow. Sediment transport pathways operate under these conditions of extreme cold and aridity and the processes involved generate permafrost landforms that are recognizable from spacecraft at local, regional and global scales. Thermal-contraction-crack polygons are associated with hemisphere-spanning mantle units that contain excess ice in the immediate subsurface. Sublimation is the dominant phase transition rather than melting under present Martian conditions. Evidence is presented for melting of near-surface snow, frost and/or ground ice in protected gully alcove microclimates during the most recent several million years.
Stratified slope deposits: periglacial and other processes involved
Authors: Van Steijn H.
Published: May 2011

Stratified slope deposits occur on or at the base of slopes within a wide range of hilly or mountainous environments. Gelifraction, a lack of vegetation, and snowmelt-derived slopewash are thought to be important factors in their development. The relatively fine-grained
grèzes litées
found at many sites within the French Charente region are the prototype of this kind of deposit. The original, strictly limited and genetically intended definition has been enlarged by mainly non-French authors causing considerable confusion.

Other assemblages of cold-climate slope deposits are more heterogeneous, sometimes less clearly stratified, and show much larger variation in grain sizes and sedimentary structures than
grèzes litées
. The most important of these are stone-banked solifluction (sheets or lobes), rock fall, (dry) grain flow, frost-coated clast flow, debris flow, snow avalanching and, to a lesser degree, rain-generated overland flow and (niveo-) aeolian activity. Many of these processes are azonal and occur under a wide range of environmental conditions. A periglacial context may nevertheless influence process activity in these cases, for instance with regard to their magnitude and/or frequency.
Cold-climate slope deposits and landscape modifications of the Mid-Atlantic Coastal Plain, Eastern USA
Authors: Newell W. L. , Dejong B. D.
Published: May 2011

The effects of Pleistocene cold-climate geomorphology are distributed across the weathered and eroded Mid-Atlantic Coastal Plain uplands from the Wisconsinan terminal moraine south to Tidewater Virginia. Cold-climate deposits and landscape modifications are superimposed on antecedent landscapes of old, weathered Neogene upland gravels and Pleistocene marine terraces that had been built during warm periods and sea-level highstands. In New Jersey, sequences of surficial deposits define a long history of repeating climate change events. To the south across the Delmarva Peninsula and southern Maryland, most antecedent topography has been obscured by Late Pleistocene surficial deposits. These are spatially variable and are collectively described as a cold-climate alloformation. The cold-climate alloformation includes time-transgressive details of climate deterioration from at least marine isotope stage (MIS) 4 through the end of MIS 2. Some deposits and landforms within the alloformation may be as young as the Younger Dryas. Southwards along the trend of the Potomac River, these deposits and their climatic affinities become diffused. In Virginia, a continuum of erosion and surficial deposits appears to be the product of ‘normal’ temperate, climate-forced processes. The cold-climate alloformation and more temperate deposits in Virginia are being partly covered by Holocene alluvium and bay mud.
Fingerprints of Quaternary glaciations on Svalbard
Authors: Ingólfsson Ó.
Published: May 2011

Marine and terrestrial archives can be used to reconstruct the development of glacially influenced depositional environments on Svalbard in time and space during the late Cenozoic. The marine archives document sedimentary environments, deposits and landforms associated with the Last Glacial Maximum (LGM) when Svalbard and the Barents Sea were covered by continental-scale marine-based ice sheet, the last deglaciation and the work of tidewater glaciers in interglacial setting as today. The terrestrial archives record large-scale Quaternary glacial sculpturing and repeated build-up and decay of the Svalbard–Barents Sea ice sheet. The fingerprinting of Quaternary glaciations on Svalbard reflects the transition from a full-glacial mode, with very extensive coverage by the Svalbard–Barents Sea ice sheet and subsequent deglaciation, to an interglacial mode with valley, cirque and tidewater glaciers as active agents of erosion and deposition. Conceptual models for Svalbard glacial environments are useful for understanding developments of glacial landforms and sediments in formerly glaciated areas. Svalbard glacial environments, past and present, may serve as analogues for interpreting geological records of marine-terminating and marine-based ice sheets in the past.
The kinematics of ancient tidewater ice margins: criteria for recognition from grounding-line moraines
Authors: Lønne Ida , Nemec W.
Published: May 2011

Modern studies indicate that the kinematic behaviour of tidewater glaciers is a crucial part of ice-sheet dynamics. A similar relationship may be expected for ancient ice sheets, but can the kinematics of ancient tidewater ice margins be recognized? The paper addresses this methodological issue by pointing to the high-resolution stratigraphic record of marine moraines, thus far little explored. On the basis of a series of case studies, a range of field criteria are proposed for the recognition of short-term grounding-line movement and possible oscillations in moraine outcrop sections. The method combines allostratigraphic mapping and architectural facies analysis of the moraine sedimentary units formed during glacier advance, subaqueous stillstand and eventual subaerial stillstand, with recognition of the successive ice-contact surfaces. The stacking architecture of the sedimentary units and their ice-contact bounding surfaces reveal the time–distance trajectory of the grounding-line positions. It is also possible to recognize changes in the mode and rate of subglacial sediment delivery, as well as fluctuations in the ice flux and meltwater discharge. This methodology invites detailed studies of marine moraines. Systematic case studies on a regional scale may provide new information on the behaviour of tidewater ice margins and lead to unprecedented insights into the dynamics of ancient ice sheets.
The formation of Alpine lateral moraines inferred from sedimentology and radar reflection patterns: a case study from Gornergletscher, Switzerland
Authors: Lukas S. , Sass O.
Published: May 2011

Studies on the formation of alpine lateral moraines are rare; consequently, their internal structure and their modes of formation are relatively poorly understood. We present here sedimentological and ground-penetrating radar (GPR) data from a lateral moraine complex where an exposure allows radar facies to be compared to the field evidence. The moraine, which is slightly asymmetric with a slightly steeper distal slope, consists of alternating clast- and matrix-supported stratified diamicts and intercalated sorted sediment units which are all subparallel to the moraine surface. They are interpreted as subaerial debris flows and fluvial sediments, respectively. The GPR, using frequencies of 50, 100 and 200 MHz, allows tracing of very similar subparallel reflectors to
c.
10 m into the subsurface. These are interpreted to represent the diamict units while the thickness of the sorted sediment units is below GPR resolution. Our data suggests that a two or three frequency approach supplemented by common-midpoint (CMP) measurements works well in glaciated high-mountain environments. Based on our sedimentological and GPR data we develop a conceptual framework for the formation of successive lateral moraines which involves ice-contact fan sedimentation, followed by collapse and incremental reworking of the proximal side during glacier retreat.
Palaeozoic glacial depositional environments of SW Saudi Arabia: process and product
Authors: Keller Martin , Hinderer Matthias , Al-Ajmi Hussain , Rausch Randolf
Published: May 2011

Saudi Arabia is one of the few places in Gondwana where deposits of two Palaeozoic glaciations are well preserved. They were formed under similar conditions in a tectonically stable shelf setting. We studied the sedimentary facies and architectural geometries of Upper Ordovician and Permo-Carboniferous glaciogenic deposits in the Wajid Sandstone and developed a genetic model for both units. Common features and of both glacial episodes are (a) incision of glacial (tunnel?) valleys, (b) subsequent valley fill by proglacial deposits in front of an oscillating, polythermal ice shield; (c) intra-formational erosional events through repeated ice advance, (d) widespread and large-scale soft deformation due to glacial surge during deglaciation, and (e) marine transgression most probably due to eustatic sea-level rise following deglaciation. The general patterns and observations fit well with recently published observations on Upper Ordovician deposits in northern Africa, pointing to closely coupled glaciological processes of the North African–Arabian ice shield. Although the same general patterns apply for the Permo-Carboniferous glaciation, sedimentary styles and petrographical properties differ, probably due to a more heterogeneous ice-flow pattern controlled by a more pronounced topography after the Hercynian tectonic event.
Frozen sediments and previously-frozen sediments
Authors: French H.
Published: May 2011

Frozen surficial sediments that are otherwise unconsolidated contain structures and characteristics that are different from those of the same sediments when in an unfrozen state. These differences are usually related to either the nature of the ice contained within the frozen sediment or to weathering processes and chemical precipitates that are associated with freezing and thawing. This paper summarizes (a) the manner in which ground freezes when a landscape experiences the onset of cold-climate conditions and (b) what happens when newly transported sediments freeze following deposition in that environment. In the absence of obvious morphological evidence, the recognition of previously-frozen sediments is problematic. Less well-understood evidence includes secondary precipitates, neoformed clay minerals, seasonal frost cracks and fragipans.
Aging is associated with hypoxia and oxidative stress in adipose tissue: implications for adipose function
Authors: Zhang Le , Ebenezer Philip J. , Dasuri Kalavathi , Fernandez-Kim Sun Ok , Francis Joseph , Mariappan Nithya , Gao Zhanguo , Ye Jianping , Bruce-Keller Annadora J. , Keller Jeffrey N.
Published: May 2011

As a part of aging there are known to be numerous alterations which occur in multiple tissues of the body, and the focus of this study was to determine the extent to which oxidative stress and hypoxia occur during adipose tissue aging. In our studies we demonstrate for the first time that aging is associated with both hypoxia (38% reduction in oxygen levels, Po2 21.7 mmHg) and increases reactive oxygen species in visceral fat depots of aging male C57Bl/6 mice. Interestingly, aging visceral fat depots were observed to have significantly less change in the expression of genes involved in redox regulation compared with aging subcutaneous fat tissue. Exposure of 3T3-L1 adipocytes to the levels of hypoxia observed in aging adipose tissue was sufficient to alter multiple aspects of adipose biology inducing increased levels of in insulin-stimulated glucose uptake and decreased lipid content. Taken together, these data demonstrate that hypoxia and increased levels of reactive oxygen species occur in aging adipose tissue, highlighting the potential for these two stressors as potential modulators of adipose dysfunction during aging.
Pharmacological inhibition of Kv1.3 fails to modulate insulin sensitivity in diabetic mice or human insulin-sensitive tissues
Authors: Straub Stephen V. , Perez Sylvie M. , Tan Beijing , Coughlan Kimberly A. , Trebino Catherine E. , Cosgrove Patricia , Buxton Joanne M. , Kreeger John M. , Jackson V. Margaret
Published: May 2011

Genetic ablation of the voltage-gated potassium channel Kv1.3 improves insulin sensitivity and increases metabolic rate in mice. Inhibition of Kv1.3 in mouse adipose and skeletal muscle is reported to increase glucose uptake through increased GLUT4 translocation. Since Kv1.3 represents a novel target for the treatment of diabetes, the present study investigated whether Kv1.3 is functionally expressed in human adipose and skeletal muscle and whether specific pharmacological inhibition of the channel is capable of modulating insulin sensitivity in diabetic mouse models. Voltage-gated K+channel currents in human skeletal muscle cells (SkMC) were insensitive to block by the specific Kv1.3 blockers 5-(4-phenoxybutoxy)psoralen (PAP-1) and margatoxin (MgTX). Glucose uptake into SkMC and mouse 3T3-L1 adipocytes was also unaffected by treatment with PAP-1 or MgTX. Kv1.3 protein expression was not observed in human adipose or skeletal muscle from normal and type 2 diabetic donors. To investigate the effect of specific Kv1.3 inhibition on insulin sensitivity in vivo, PAP-1 was administered to hyperglycemic mice either acutely or for 5 days prior to an insulin tolerance test. No effect on insulin sensitivity was observed at free plasma PAP-1 concentrations that are specific for inhibition of Kv1.3. Insulin sensitivity was increased only when plasma concentrations of PAP-1 were sufficient to inhibit other Kv1 channels. Surprisingly, acute inhibition of Kv1.3 in the brain was found to decrease insulin sensitivity in ob/ob mice. Overall, these findings are not supportive of a role for Kv1.3 in the modulation of peripheral insulin sensitivity.
Opposite effects of serum- and glucocorticoid-regulated kinase-1 and glucocorticoids on POMC transcription and ACTH release
Authors: Reiter Marie Helene , Vila Greisa , Knosp Engelbert , Baumgartner-Parzer Sabina M. , Wagner Ludwig , Stalla Günter K. , Luger Anton
Published: May 2011

Serum- and glucocorticoid-regulated kinase-1 (SGK1) is a glucocorticoid early-response gene; its function, however, has been elucidated mainly in the context of mineralocorticoid signaling. Here, we investigate the expression and function of SGK1 in the pituitary gland, one of the primary glucocorticoid targets. SGK1 is expressed in the human pituitary gland and colocalizes to ACTH. The AtT-20 murine corticotroph cell line was used for functional experiments. Glucocorticoids upregulated SGK1 mRNA and protein levels, parallel to decreasing proopiomelanocortin (POMC) transcription and ACTH release. Dexamethasone-induced changes in SGK1 protein were abolished by the steroid receptor antagonist RU-486 and reduced by the inhibition of PI 3-kinase with LY-294002. SGK1 overexpression increased CREB- and activator protein-1-dependent transcription, POMC transcription, and ACTH secretion but did not influence intracellular cAMP levels. SGK1 overexpression and corticotropin-releasing hormone had additive effects on POMC promoter activity but not on ACTH secretion. SGK1 knockdown by RNA interference decreased POMC promoter activity, demonstrating the importance of SGK1 for basal POMC signaling. In summary, SGK1 is strongly stimulated by glucocorticoids in pituitary corticotrophs; however, its effects on POMC transcription are antagonistic to the classical inhibitory glucocorticoid action, suggesting a cell-regulated counterregulatory mechanism to potentially detrimental glucocorticoid effects.
Insulin and insulin signaling play a critical role in fat induction of insulin resistance in mouse
Authors: Ning Jie , Hong Tao , Yang Xuefeng , Mei Shuang , Liu Zhenqi , Liu Hui-Yu , Cao Wenhong
Published: May 2011

The primary player that induces insulin resistance has not been established. Here, we studied whether or not fat can cause insulin resistance in the presence of insulin deficiency. Our results showed that high-fat diet (HFD) induced insulin resistance in C57BL/6 (B6) mice. The HFD-induced insulin resistance was prevented largely by the streptozotocin (STZ)-induced moderate insulin deficiency. The STZ-induced insulin deficiency prevented the HFD-induced ectopic fat accumulation and oxidative stress in liver and gastrocnemius. The STZ-induced insulin deficiency prevented the HFD- or insulin-induced increase in hepatic expression of long-chain acyl-CoA synthetases (ACSL), which are necessary for fatty acid activation. HFD increased mitochondrial contents of long-chain acyl-CoAs, whereas it decreased mitochondrial ADP/ATP ratio, and these HFD-induced changes were prevented by the STZ-induced insulin deficiency. In cultured hepatocytes, we observed that expressions of ACSL1 and -5 were stimulated by insulin signaling. Results in cultured cells also showed that blunting insulin signaling by the PI3K inhibitor LY-294002 prevented fat accumulation, oxidative stress, and insulin resistance induced by the prolonged exposure to either insulin or oleate plus sera that normally contain insulin. Finally, knockdown of the insulin receptor prevented the oxidative stress and insulin resistance induced by the prolonged exposure to insulin or oleate plus sera. Together, our results show that insulin and insulin signaling are required for fat induction of insulin resistance in mice and cultured mouse hepatocytes.
Angiotensin II-mediated biphasic regulation of proximal tubular Na+/H+ exchanger 3 is impaired during oxidative stress
Authors: Banday Anees Ahmad , Lokhandwala Mustafa F.
Published: May 2011

Angiotensin (ANG) II via AT1 receptors (AT1Rs) maintains sodium homeostasis by regulating renal sodium transporters including Na+/H+ exchanger 3 (NHE3) in a biphasic manner. Low-ANG II concentration stimulates whereas high concentrations inhibit NHE3 activity. Oxidative stress has been shown to upregulate AT1R function that could modulate the ANG II-mediated NHE3 regulation. This study was designed to identify the signaling pathways responsible for ANG II-mediated biphasic regulation of proximal tubular NHE3 and the effect of oxidative stress on this phenomenon. Male Sprague-Dawley rats were chronically treated with a pro-oxidant l-buthionine sulfoximine (BSO) with and without an antioxidant tempol in tap water for 3 wk. BSO-treated rats exhibited oxidative stress and high blood pressure. At low concentration (1 pM) ANG II increased NHE3 activity in proximal tubules from all animals. However, in BSO-treated rats, the stimulation was more robust and was normalized by tempol treatment. ANG II (1 pM)-mediated NHE3 activation was abolished by AT1R blocker, intracellular Ca2+ chelator, and inhibitors of phospholipase C (PLC) and Ca2+-dependent calmodulin (CaM) but it was insensitive to Giα and protein kinase C inhibitors or AT2R antagonist. A high concentration of ANG II (1 μM) inhibited NHE3 activity in control and tempol-treated rats. However, in BSO-treated rats, ANG II (1 μM) continued to induce NHE3 stimulation. Tempol restored the inhibitory effect of ANG II (1 μM) in BSO-treated rats. The inhibitory effect of ANG II (1 μM) involved AT1R-dependent, cGMP-dependent protein kinase (PKG) activation and was independent of AT2 receptor and nitric oxide signaling. We conclude that ANG II stimulates NHE3 via AT1R-PLC-CaM pathway and inhibits NHE3 by AT1R-PKG activation. Oxidative stress impaired ANG II-mediated NHE3 biphasic response in that stimulation was observed at both high- and low-ANG II concentration.
SIRT1 activation by resveratrol ameliorates cisplatin-induced renal injury through deacetylation of p53
Authors: Kim Duk Hoon , Jung Yu Jin , Lee Jung Eun , Lee Ae Sin , Kang Kyung Pyo , Lee Sik , Park Sung Kwang , Han Myung Kwan , Lee Sang Yong , Ramkumar Kunga Mohan , Sung Mi Jeong , Kim Won
Published: May 2011

Nephrotoxicity is one of the important dose-limiting factors during cisplatin treatment. There is a growing body of evidence that activation of p53 has a critical role in cisplatin-induced renal apoptotic injury. The nicotinamide adenine dinucleotide-dependent protein deacetylase SIRT1 decreases apoptosis through deacetylating of p53, and resveratrol is known as an activator of SIRT1. To study the role of SIRT1 in cisplatin-induced renal injury through interaction with p53, mouse proximal tubular cells (MPT) were treated with cisplatin and examined the expression level of SIRT1, acetylation of p53, PUMA-α, Bax, the cytosolic/mitochondrial cytochrome c ratio, and active caspase-3. The expression of SIRT1 was decreased by cisplatin. Resveratrol, a SIRT1 activator, ameliorated cisplatin-induced acetylation of p53, apoptosis, and cytotoxicity in MPT cells. In addition, resveratrol remarkably blocked cisplatin-induced decrease of Bcl-xL in MPT cells. Further specific SIRT1 inhibition with EX 527 or small interference RNA specific to SIRT1 reversed the effect of resveratrol on cisplatin-induced toxicity. Inhibition of p53 by pifithrin-α reversed the effect of EX527 in protein expression of PUMA-α, Bcl-xL, and caspase-3 and cytotoxicity in MPT cells. SIRT1 protein expression after cisplatin treatment was significantly decreased in the kidney. SIRT1 activation by resveratrol decreased cisplatin-induced apoptosis while improving the glomerular filtration rate. Taken together, our findings suggest that the modulation of p53 by SIRT1 could be a possible target to attenuate cisplatin-induced kidney injury.
Involvement of Runx3 in the basal transcriptional activation of the mouse angiotensin II type 1 receptor-associated protein gene
Authors: Matsuda Miyuki , Tamura Kouichi , Wakui Hiromichi , Dejima Toru , Maeda Akinobu , Ohsawa Masato , Kanaoka Tomohiko , Haku Sona , Azushima Kengo , Yamasaki Hiroko , Saito Daisuke , Hirose Tomonori , Maeshima Yohei , Nagashima Yoji , Umemura Satoshi
Published: May 2011

We previously cloned a molecule that interacts with angiotensin II type 1 (AT1) receptor to exert an inhibitory function on AT1 receptor signaling that we named ATRAP/ Agtrap (for AT1 receptor-associated protein). In the present study we examined the regulation of basal ATRAP gene expression using renal distal convoluted tubule cells. We found that serum starvation upregulated basal expression of ATRAP gene, a response that required de novo mRNA and protein synthesis. Luciferase assay revealed that the proximal promoter region directs transcription and that a putative binding site of runt-related transcription factors (RBE) is important for transcriptional activation. The results of RBE-decoy transfection and endogenous knockdown by small interference RNA showed that the runt-related transcription factor Runx3 is involved in ATRAP gene expression. Chromatin immunoprecipitation assay also supported the binding of Runx3 to the ATRAP promoter in renal distal convoluted tubule cells. Immunohistochemistry demonstrated the expression of Runx3 and ATRAP proteins in the distal convoluted and connecting tubules of the kidney in consecutive sections. Furthermore, the Runx3 immunostaining was decreased together with a concomitant suppression of ATRAP expression in the affected kidney after 7 days of unilateral ureteral obstruction. These findings indicate that Runx3 plays a role in ATRAP gene expression in renal distal tubular cells both in vitro and in vivo.
Origins of interspecies variation in mammalian muscle metabolic enzymes
Authors: Kocha K. M. , Genge C. E. , Moyes C. D.
Published: May 2011

Do the transcriptional mechanisms that control an individual's mitochondrial content, PGC1α (peroxisome proliferator-activated receptor γ coactivator-1α) and NRF1 (nuclear respiratory factor-1), also cause differences between species? We explored the determinants of cytochrome c oxidase (COX) activities in muscles from 12 rodents differing 1,000-fold in mass. Hindlimb muscles differed in scaling patterns from isometric (soleus, gastrocnemius) to allometric (tibialis anterior, scaling coefficient = −0.16). Consideration of myonuclear domain reduced the differences within species, but interspecies differences remained. For tibialis anterior, there was no significant scaling relationship in mRNA/g for COX4-1, PGC1α, or NRF1, yet COX4-1 mRNA/g was a good predictor of COX activity (r2 = 0.55), PGC1α and NRF1 mRNA correlated with each other (r2 = 0.42), and both could predict COX4-1 mRNA (r2 = 0.48 and 0.52) and COX activity (r2 = 0.55 and 0.49). This paradox was resolved by multivariate analysis, which explained 90% of interspecies variation, about equally partitioned between mass effects and PGC1α (or NRF1) mRNA levels, independent of mass. To explore the determinants of PGC1α mRNA, we analyzed 52 mammalian PGC1α proximal promoters and found no size dependence in regulatory element distribution. Likewise, the activity of PGC1α promoter reporter genes from 30 mammals showed no significant relationship with body mass. Collectively, these studies suggest that not all muscles scale equivalently, but for those that show allometric scaling, transcriptional regulation of the master regulators, PGC1α and NRF1, does not account for scaling patterns, though it does contribute to interspecies differences in COX activities independent of mass.
Severe Hypocalcemia due to Vitamin D Deficiency after Extended Roux-en-Y Gastric Bypass
Authors: Miñambres I. , Chico A. , Pérez A.
Published: May 2011

Vitamin D deficiency is a well-known comorbidity of obesity that can be exacerbated after bariatric surgery and can predispose the patient for hypocalcemia. Vitamin D and calcium doses to prevent and treat vitamin D deficiency after weight loss surgery are not well defined. We describe a patient who developed severe hypocalcemia due to vitamin D deficiency 5 years after an extended Roux-en-Y gastric bypass for a type II obesity. No precipitating factors were present and malabsorption induced by the bypass was considered to be the main causative factor. High doses of vitamin D and calcium were needed to reach and maintain normal calcium and vitamin D concentrations. This case emphasises the importance of routine screening for vitamin D deficiency in obese individuals and reflects that while consensus does not exist regarding optimal dosage, vitamin D replacement should be tittered based on calcidiol levels.
Fine Spectra of Tridiagonal Symmetric Matrices
Authors: Altun Muhammed
Published: May 2011

The fine spectra of upper and lower triangular banded matrices were examined by several authors. Here we determine the fine spectra of tridiagonal symmetric infinite matrices and also give the explicit form of the resolvent operator for the sequence spaces c0, c, ℓ1, and ℓ∞.
Effects of Secondhand Smoke Exposure on the Health and Development of African American Premature Infants
Authors: Brooks Jada , Holditch-Davis Diane , Weaver Mark A. , Miles Margaret Shandor , Engelke Stephen C.
Published: May 2011

Objective. To explore the effects of secondhand smoke exposure on growth, health-related illness, and child development in rural African American premature infants through 24 months corrected age.Method. 171 premature infants (72 boys, 99 girls) of African American mothers with a mean birthweight of 1114 grams. Mothers reported on household smoking and infant health at 2, 6, 12, 18, and 24 months corrected age. Infant growth was measured at 6, 12, 18, and 24 months, and developmental assessments were conducted at 12 and 24 months.Results. Thirty percent of infants were exposed to secondhand smoke within their first 2 years of life. Secondhand smoke exposure was associated with poorer growth of head circumference and the development of otitis media at 2 months corrected age. Height, weight, wheezing, and child development were not related to secondhand smoke exposure.Conclusion. Exposure to secondhand smoke may negatively impact health of rural African American premature infants. Interventions targeted at reducing exposure could potentially improve infant outcomes.
Variations in Adipokine GenesAdipoQ,Lep, andLepRAre Associated with Risk for Obesity-Related Metabolic Disease: The Modulatory Role of Gene-Nutrient Interactions
Authors: Enns Jennifer Emily , Taylor Carla G. , Zahradka Peter
Published: May 2011

Obesity rates are rapidly increasing worldwide and facilitate the development of many related disease states, such as cardiovascular disease, the metabolic syndrome, type 2 diabetes mellitus, and various types of cancer. Variation in metabolically important genes can have a great impact on a population's susceptibility to becoming obese and/or developing related complications. The adipokines adiponectin and leptin, as well as the leptin receptor, are major players in the regulation of body energy homeostasis and fat storage. This paper summarizes the findings of single nucleotide polymorphisms in these three genes and their effect on obesity and metabolic disease risk. Additionally, studies of gene-nutrient interactions involving adiponectin, leptin, and the leptin receptor are highlighted to emphasize the critical role of diet in susceptible populations.
CD8 T Cells andToxoplasma gondii: A New Paradigm
Authors: Gigley Jason P. , Bhadra Rajarshi , Khan Imtiaz A.
Published: May 2011

CD8 T cells are essential for control ofToxoplasma gondiiinfection. Once activated they undergo differentiation into short-lived effector and memory precursor effector cells. As effector cells, CD8 T cells exert immune pressure on the parasite via production of inflammatory cytokines and through their cytolytic activity. Once immune control has been established, the parasite encysts and develops into chronic infection regulated by the memory CD8 T-cell population. Several signals are needed for this process to be initiated and for development of fully differentiated memory CD8 T cells. With newly developed tools including CD8 T-cell tetramers and TCR transgenic mice, dissecting the biology behindT. gondii-specific CD8 T-cell responses can now be more effectively addressed. In this paper, we discuss what is known about the signals required for effectiveT. gondii-specific CD8 T-cell development, their differentiation, and effector function.
The Metabolic Syndrome and Mind-Body Therapies: A Systematic Review
Authors: Anderson Joel G. , Taylor Ann Gill
Published: May 2011

The metabolic syndrome, affecting a substantial and increasing percentage of the worldwide population, is comprised of a cluster of symptoms associated with increased risk of type 2 diabetes, cardiovascular disease, and other chronic conditions. Mind-body modalities based on Eastern philosophy, such as yoga, tai chi, qigong, and meditation, have become increasingly popular worldwide. These complementary therapies have many reported benefits for improving symptoms and physiological measures associated with the metabolic syndrome. However, clinical trial data concerning the effectiveness of these practices on the syndrome as a whole have not been evaluated using a systematic and synthesizing approach. A systematic review was conducted to critically evaluate the data from clinical trials examining the efficacy of mind-body therapies as supportive care modalities for management of the metabolic syndrome. Three clinical trials addressing the use of mind-body therapies for management of the metabolic syndrome were identified. Findings from the studies reviewed support the potential clinical effectiveness of mind-body practices in improving indices of the metabolic syndrome.
An ELISA to Detect Serum Antibodies to the Salivary Gland Toxin ofIxodes holocyclusNeumann in Dogs and Rodents
Authors: Hall-Mendelin S. , O'Donoghue P. , Atwell R. B. , Lee R. , Hall R. A.
Published: May 2011

TheIxodes holocyclustick causes paralysis in up to 10,000 companion and domestic animals each year in Australia. Treatment requires the removal of the parasite and the administration of a commercial tick antiserum that is prepared from hyperimmune dogs. Each batch of this serum is initially tested for toxin-neutralising potency in a mouse bioassay that is expensive, time consuming, and subjective. With the aim of developing a rapidin vitroassay to replace the bioassay, we used a partially purified antigen prepared fromI. holocyclussalivary glands to develop an ELISA to detect toxin-reactive antibodies in hyperimmune dog sera. The optimised ELISA reliably detected antibodies reactive toI. holocyclussalivary gland antigens. Parallel testing of sera with a negative control antigen prepared from the salivary glands of the nontoxic tickRhipicephalus(Boophilus)microplusprovided further evidence that we were detecting toxin-specific antibodies in the assay. Using the ELISA, we could also detect antibodies induced in rats after experimental infestation withI. holocyclus. This assay shows promise as an alternative means of assessing the potency of batches of hyperimmune dog serum and to screen for toxin-reactive monoclonal antibodies produced from immunised rodents.
Synthesis and Crystal Structure Differences between Fully and Partially Fluorinated β-Diketonate Metal (Co2+, Ni2+, and Cu2+) Complexes
Authors: Hori Akiko , Mizutani Masaya
Published: May 2011

Coordination complexes, [Co2(1)4(H2O)2] (2), [Ni2(1)4(H2O)2] (3), and [Cu(1)2] (4), by using an asymmetric and partially fluorinated 3-hydroxy-3-pentafluorophenyl-1-phenyl-2-propen-1-one (H1) have been prepared, and the structures were investigated to compare with the corresponding fully fluorinated complexes of [Co2(5)4(H2O)2] (6), [Ni2(5)4(H2O)2] (7), and [Cu(5)2] (8) with bis(pentafluorobenzoyl)methane (H5) and to understand the fluorine-substituted effects. While the coordination mode of the partially fluorinated complexes was quite similar to the fully fluorinated complexes, the intra- and intermolecular π-interactions of the ligand moieties were highly influenced by the fluorination effects; the arene-perfluoroarene interactions were observed in complexes 2 and 3 as a reason of the dinucleation. In this paper, we describe detail structures of the protonated form of the ligand, H1, and complexes 2–4 by X-ray crystallographic studies.
Myasthenia Gravis Development and Crisis Subsequent to Multiple Sclerosis
Authors: Gharagozli Kurosh , Shojaei Maziar , Harandi Ali Amini , Akbari Nayyereh , Ilkhani Manouchehr
Published: May 2011

During the last decade, sporadic combination of multiple sclerosis (MS) and myasthenia gravis (MG) has been reported repeatedly. Although these are anecdotal, they are important enough to raise concerns about co-occurrence of MG and MS. Here, we present a case of an MS patient who developed an MG crisis. She had received interferon for relapsing remitting MS. Interestingly, she developed an MG crisis 4 years after the diagnosis of MS. MS and MG have relatively the same distribution for age, corresponding to the younger peak of the bimodal age distribution in MG. They also share some HLA typing characteristics. Furthermore, some evidences support the role of systemic immune dysregulation due to a genetic susceptibility that is common to these two diseases. The association may be underdiagnosed because of the possible overlap of symptoms especially bulbar manifestations in which either MG or MS can mimic each other, leading to underestimating incidence of the combination. The evidence warrants physicians, especially neurologists, to always consider the possibility of the other disease when encountering any patients either with MS or MG. Anecdotal and sporadic reports of combination of multiple sclerosis (MS) and myasthenia gravis (MG) have been raised concerns about co-occurrence of them.
Effects onGlomus mosseaeRoot Colonization byPaenibacillus polymyxaandPaenibacillus brasilensisStrains as Related to Soil P-Availability in Winter Wheat
Authors: Arthurson Veronica , Hjort Karin , Muleta Diriba , Jäderlund Lotta , Granhall Ulf
Published: May 2011

Greenhouse experiments were conducted to assess the effects of inoculating winter wheat (Triticum aestivum) with plant growth promoting rhizobacteria (PGPR) of the genusPaenibacillusunder phosphate P-limited soil conditions in the presence or absence of the arbuscular mycorrhizal fungus (AMF)Glomus mosseae. FourP. polymyxastrains and oneP. brasilensisstrain were compared at two cell concentrations (106and 108 cells g−1seeds) of inoculation, and surface sterilized AMF spores were added to pots. Mycorrhizal root colonization, plant growth, and plant uptake of phosphorus were analyzed. Bacterial phosphate solubilization was examined separatelyin vitro. MostP. polymyxastrains, isolated from wheat, had dramatic effectsper seon root growth and root P-content. No treatment gave significant effect on shoot growth. AMF root colonization levels and total plant uptake of P were much stimulated by the addition of mostP. polymyxastrains. The AM fungus alone and theP. brasilensis, alone or in combination with the fungus, did not affect total plant P-levels. Our results indicate that practical application of inoculation with plant host-specific rhizobacteria (i.e.,P. polymyxa) could positively influence uptake of phosphorus in P-deficient soils by wheat plants, provided that suitable AM fungi (e.g.,G. mosseae) are present.
The Rieske Iron-Sulfur Protein: Import and Assembly into the Cytochrome Complex of Yeast Mitochondria
Authors: Conte Laura , Zara Vincenzo
Published: May 2011

The Rieske iron-sulfur protein, one of the catalytic subunits of the cytochrome complex, is involved in electron transfer at the level of the inner membrane of yeast mitochondria. The Rieske iron-sulfur protein is encoded by nuclear DNA and, after being synthesized in the cytosol, is imported into mitochondria with the help of a cleavable N-terminal presequence. The imported protein, besides incorporating the 2Fe-2S cluster, also interacts with other catalytic and non-catalytic subunits of the cytochrome complex, thereby assembling into the mature and functional respiratory complex. In this paper, we summarize the most recent findings on the import and assembly of the Rieske iron-sulfur protein intoSaccharomyces cerevisiaemitochondria, also discussing a possible role of this protein both in the dimerization of the cytochrome complex and in the interaction of this homodimer with other complexes of the mitochondrial respiratory chain.
Phosphorylation: The Molecular Switch of Double-Strand Break Repair
Authors: Summers K. C. , Shen F. , Sierra Potchanant E. A. , Phipps E. A. , Hickey R. J. , Malkas L. H.
Published: May 2011

Repair of double-stranded breaks (DSBs) is vital to maintaining genomic stability. In mammalian cells, DSBs are resolved in one of the following complex repair pathways: nonhomologous end-joining (NHEJ), homologous recombination (HR), or the inclusive DNA damage response (DDR). These repair pathways rely on factors that utilize reversible phosphorylation of proteins as molecular switches to regulate DNA repair. Many of these molecular switches overlap and play key roles in multiple pathways. For example, the NHEJ pathway and the DDR both utilize DNA-PK phosphorylation, whereas the HR pathway mediates repair with phosphorylation of RPA2, BRCA1, and BRCA2. Also, the DDR pathway utilizes the kinases ATM and ATR, as well as the phosphorylation of H2AX and MDC1. Together, these molecular switches regulate repair of DSBs by aiding in DSB recognition, pathway initiation, recruitment of repair factors, and the maintenance of repair mechanisms.
Applying Sewage Sludge toEucalyptus grandisPlantations: Effects on Biomass Production and Nutrient Cycling through Litterfall
Authors: da Silva Paulo Henrique Müller , Poggiani Fabio , Laclau Jean Paul
Published: May 2011

In most Brazilian cities sewage sludge is dumped into sanitary landfills, even though its use in forest plantations as a fertilizer and soil conditioner might be an interesting option. Sewage sludge applications might reduce the amounts of mineral fertilizers needed to sustain the productivity on infertile tropical soils. However, sewage sludge must be applied with care to crops to avoid soil and water pollution. The aim of our study was to assess the effects of dry and wet sewage sludges on the growth and nutrient cycling ofEucalyptus grandisplantations established on the most common soil type for Brazilian eucalypt plantations. Biomass production and nutrient cycling were studied over a 36-month period in a complete randomized block design. Four experimental treatments were compared: wet sewage sludge, dry sludge, mineral fertilizer, and no fertilizer applications. The two types of sludges as well as mineral fertilizer increased significantly the biomass ofEucalyptustrees. Wood biomass productions 36 months after planting were similar in the sewage sludge and mineral fertilization treatments (about 80 tons ha−1) and 86% higher than in the control treatment. Sewage sludge application also affected positively leaf litter production and significantly increased nutrient transfer among the components of the ecosystem.
A Matrix Formulation for the Moment Distribution Method Applied to Continuous Beams
Authors: Lopes Arlindo Pires , Santos Adriana Alencar , Lopes Rogério Coelho
Published: May 2011

The Moment Distribution Method is a quite powerful hand method of structural analysis, in which the solution is obtained iteratively without even formulating the equations for the unknowns. It was formulated by Professor Cross in an era where computer facilities were not available to solve frame problems that normally require the solution of simultaneous algebraic equations. Its relevance today, in the era of personal computers, is in its insight on how a structure reacts to applied loads by rotating its nodes and thus distributing the loads in the form of member-end moments. Such an insight is the foundation of the modern displacement method. This work has a main objective to present an exact solution for the Moment Distribution Method through a matrix formulation using only one equation. The initial moments at the ends of the members and the distribution and carry-over factors are calculated from the elementary procedures of structural analysis. Four continuous beams are investigated to illustrate the applicability and accuracy of the proposed formulation. The use of a matrix formulation yields excellent results when compared with those in the literature or with a commercial structural program.
Iatrogenic Hepatic Pneumovenogram
Authors: Rajendiran Govarthanan , Rathore Sulaiman , Sidhu Gurmeet , Catevenis James
Published: May 2011

Accidental air entry during central venous catheterization is a preventable iatrogenic complication that can cause venous air embolism (VAE). Many cases of VAE are subclinical with no adverse outcome and thus go unreported. Usually, when symptoms are present, they are nonspecific, and a high index of clinical suspicion of possible VAE is required to prompt investigations and initiate appropriate therapy. Occasionally large embolism can lead to life-threatening acute cor pulmonale, asystole, sudden death, and arterial air embolism in the presence of shunt or patent foramen ovale. This paper discusses VAE during emergency central line placement and the diagnostic dilemma that it can be created in critically ill patients. All necessary precautions have to be strictly followed to prevent this iatrogenic complication.
Knowledge of Toxoplasmosis among Doctors and Nurses Who Provide Prenatal Care in an Endemic Region
Authors: da Silva Laura Berriel , de Oliveira Raquel de Vasconcelos Carvalhaes , da Silva Marizete Pereira , Bueno Wendy Fernandes , Amendoeira Maria Regina Reis , Neves Elizabeth de Souza
Published: May 2011

Congenital toxoplasmosis is a potentially severe infection and its prevention is most often based on serological screening in pregnant women. Many cases could be prevented by simple precautions during pregnancy. Aiming to assess the knowledge about toxoplasmosis among professionals working in antenatal care in a high prevalent region, a questionnaire was administered to 118 obstetric nurses and physicians attending at primary care units and hospitals. The questionnaire was self-completed and included questions on diagnosis, clinical issues, and prevention. Only 44% of total answers were corrected. Lower scores were observed among those with over 10 years of graduation, working in primary care units, and nurses. Errors were mainly observed in questions of prevention and diagnosis. As congenital toxoplasmosis is a mother-to-child (MTC) transmitted disease, early diagnosis and treatment can prevent serious and irreversible fetal damage. Thus, doctors and nurses who provide prenatal care must be appropriately trained on prophylactic, diagnostic, and clinical aspects of toxoplasmosis. The authors suggest that measures should be taken for continuing education regarding toxoplasmosis in pregnancy.
Autophagic and Apoptotic Effects of HDAC Inhibitors on Cancer Cells
Authors: Rikiishi Hidemi
Published: May 2011

Because epigenetic alterations are believed to be involved in the repression of tumor suppressor genes and the promotion of tumorigenesis in cancers, novel compounds endowed with histone deacetylase (HDAC) inhibitory activity are an attractive therapeutic approach. Indeed, the potential of HDAC inhibitors for cancer therapy has been explored in preclinical models, and some agents approved for hematologic malignancies have reached the clinical setting. HDAC inhibitors are able to mediate the induction of both apoptosis and autophagy, which are related to anticancer activity in a variety of cancer cell lines. Given the inherent resistance to apoptosis that characterizes cancer, the targeting of alternative pathways is an attractive strategy to improve anti‐tumor therapy. The activation of autophagy represents novel cancer treatment targets. This paper aims to critically discuss how the anticancer potential of HDAC inhibitors may elicit a response to human cancers through different cell pathways leading to cell death.