bluuebunny/crossref_metadata_2025 huggingface dataset:
Table S2 from The Tumor Immune Microenvironment Drives a Prognostic Relevance That Correlates with Bladder Cancer Subtypes
Authors: Pfannstiel Carolin , Strissel Pamela L. , Chiappinelli Katherine B. , Sikic Danijel , Wach Sven , Wirtz Ralph M. , Wullweber Adrian , Taubert Helge , Breyer Johannes , Otto Wolfgang , Worst Thomas , Burger Maximilian , Wullich Bernd , Bolenz Christian , Fuhrich Nicole , Geppert Carol I. , Weyerer Veronika , Stoehr Robert , Bertz Simone , Keck Bastian , Erlmeier Franziska , Erben Philipp , Hartmann Arndt , Strick Reiner , Eckstein Markus
Published: April 2023

Reference Sequences - Nanostring analysis for subtyping (CCC-EMN cohort).
Table S2 from Cancer-Associated Fibroblasts Promote Immunosuppression by Inducing ROS-Generating Monocytic MDSCs in Lung Squamous Cell Carcinoma
Authors: Xiang Handan , Ramil Carlo P. , Hai Josephine , Zhang Chunsheng , Wang Huijun , Watkins Amanda A. , Afshar Roshi , Georgiev Peter , Sze Marc A. , Song Xuelei S. , Curran Patrick J. , Cheng Mangeng , Miller J. Richard , Sun Dongyu , Loboda Andrey , Jia Yanlin , Moy Lily Y. , Chi An , Brandish Philip E.
Published: April 2023

Supplementary table 2
Supplementary Figures from A Transcriptionally Distinct CXCL13+CD103+CD8+ T-cell Population Is Associated with B-cell Recruitment and Neoantigen Load in Human Cancer
Authors: Workel Hagma H. , Lubbers Joyce M. , Arnold Roland , Prins Thalina M. , van der Vlies Pieter , de Lange Kim , Bosse Tjalling , van Gool Inge C. , Eggink Florine A. , Wouters Maartje C.A. , Komdeur Fenne L. , van der Slikke Elisabeth C. , Creutzberg Carien L. , Kol Arjan , Plat Annechien , Glaire Mark , Church David N. , Nijman Hans W. , de Bruyn Marco
Published: April 2023

Supplementary Figures 1-3 with legends
Table S3 from Proteogenomics Uncovers a Vast Repertoire of Shared Tumor-Specific Antigens in Ovarian Cancer
Authors: Zhao Qingchuan , Laverdure Jean-Philippe , Lanoix Joël , Durette Chantal , Côté Caroline , Bonneil Éric , Laumont Céline M. , Gendron Patrick , Vincent Krystel , Courcelles Mathieu , Lemieux Sébastien , Millar Douglas G. , Ohashi Pamela S. , Thibault Pierre , Perreault Claude
Published: April 2023

Table S3
Supplementary Tables and Figures from MicroRNAs Affect Complement Regulator Expression and Mitochondrial Activity to Modulate Cell Resistance to Complement-Dependent Cytotoxicity
Authors: Hillman Yaron , Mardamshina Mariya , Pasmanik-Chor Metsada , Ziporen Lea , Geiger Tamar , Shomron Noam , Fishelson Zvi
Published: April 2023

Supplementary Tables 1-2 and Figures 1-3
Supplementary Array S1 from MicroRNAs Affect Complement Regulator Expression and Mitochondrial Activity to Modulate Cell Resistance to Complement-Dependent Cytotoxicity
Authors: Hillman Yaron , Mardamshina Mariya , Pasmanik-Chor Metsada , Ziporen Lea , Geiger Tamar , Shomron Noam , Fishelson Zvi
Published: April 2023

Supplementary Array S1
Overall survival of patients stratified for CCL19 serum concentration. from Accumulation of Circulating CCR7+ Natural Killer Cells Marks Melanoma Evolution and Reveals a CCL19-Dependent Metastatic Pathway
Authors: Cristiani Costanza Maria , Turdo Alice , Ventura Valeria , Apuzzo Tiziana , Capone Mariaelena , Madonna Gabriele , Mallardo Domenico , Garofalo Cinzia , Giovannone Emilia Dora , Grimaldi Antonio M. , Tallerico Rossana , Marcenaro Emanuela , Pesce Silvia , Del Zotto Genny , Agosti Valter , Costanzo Francesco Saverio , Gulletta Elio , Rizzo Aroldo , Moretta Alessandro , Karre Klas , Ascierto Paolo A. , Todaro Matilde , Carbone Ennio
Published: April 2023

Kaplan-Meier survival curves in 9 patients with low and 13 with high CCL19 serum concentration.
CSCs characterization. from Accumulation of Circulating CCR7+ Natural Killer Cells Marks Melanoma Evolution and Reveals a CCL19-Dependent Metastatic Pathway
Authors: Cristiani Costanza Maria , Turdo Alice , Ventura Valeria , Apuzzo Tiziana , Capone Mariaelena , Madonna Gabriele , Mallardo Domenico , Garofalo Cinzia , Giovannone Emilia Dora , Grimaldi Antonio M. , Tallerico Rossana , Marcenaro Emanuela , Pesce Silvia , Del Zotto Genny , Agosti Valter , Costanzo Francesco Saverio , Gulletta Elio , Rizzo Aroldo , Moretta Alessandro , Karre Klas , Ascierto Paolo A. , Todaro Matilde , Carbone Ennio
Published: April 2023

Negative controls for CD44, CD271, ABCB5, and CD166 antibodies on melanoma CSCs.
Figure S5 from Radiotherapy and Cisplatin Increase Immunotherapy Efficacy by Enabling Local and Systemic Intratumoral T-cell Activity
Authors: Kroon Paula , Frijlink Elselien , Iglesias-Guimarais Victoria , Volkov Andriy , van Buuren Marit M. , Schumacher Ton N. , Verheij Marcel , Borst Jannie , Verbrugge Inge
Published: April 2023

Cisplatin modestly reduces the RIT-induced increase in CD8:CD4 T cell ratio and enhances (R)IT-medated control of non-irradiated tumors.
Figure S4 from Radiotherapy and Cisplatin Increase Immunotherapy Efficacy by Enabling Local and Systemic Intratumoral T-cell Activity
Authors: Kroon Paula , Frijlink Elselien , Iglesias-Guimarais Victoria , Volkov Andriy , van Buuren Marit M. , Schumacher Ton N. , Verheij Marcel , Borst Jannie , Verbrugge Inge
Published: April 2023

Identification of an AT-3 tumor-specific (CD8) T cell epitope, and targeting either Ly6G or CSF1R does not improve RIT-mediated control of non-irradiated tumor.
Figure S3 from Radiotherapy and Cisplatin Increase Immunotherapy Efficacy by Enabling Local and Systemic Intratumoral T-cell Activity
Authors: Kroon Paula , Frijlink Elselien , Iglesias-Guimarais Victoria , Volkov Andriy , van Buuren Marit M. , Schumacher Ton N. , Verheij Marcel , Borst Jannie , Verbrugge Inge
Published: April 2023

Individual tumor size following radio-immunotherapy delivered as one dose or in 3 x 8 Gy fractions.
Table S3 from Human CD4+ T Cells Specific for Merkel Cell Polyomavirus Localize to Merkel Cell Carcinomas and Target a Required Oncogenic Domain
Authors: Longino Natalie V. , Yang Junbao , Iyer Jayasri G. , Ibrani Dafina , Chow I-Ting , Laing Kerry J. , Campbell Victoria L. , Paulson Kelly G. , Kulikauskas Rima M. , Church Candice D. , James Eddie A. , Nghiem Paul , Kwok William W. , Koelle David M.
Published: April 2023

Human polyomavirus LxCxE homology sequences
Table S2 from Human CD4+ T Cells Specific for Merkel Cell Polyomavirus Localize to Merkel Cell Carcinomas and Target a Required Oncogenic Domain
Authors: Longino Natalie V. , Yang Junbao , Iyer Jayasri G. , Ibrani Dafina , Chow I-Ting , Laing Kerry J. , Campbell Victoria L. , Paulson Kelly G. , Kulikauskas Rima M. , Church Candice D. , James Eddie A. , Nghiem Paul , Kwok William W. , Koelle David M.
Published: April 2023

TCR sequences of MCPyV-WED-specific CD4 T cell clones
Table S1 from Human CD4+ T Cells Specific for Merkel Cell Polyomavirus Localize to Merkel Cell Carcinomas and Target a Required Oncogenic Domain
Authors: Longino Natalie V. , Yang Junbao , Iyer Jayasri G. , Ibrani Dafina , Chow I-Ting , Laing Kerry J. , Campbell Victoria L. , Paulson Kelly G. , Kulikauskas Rima M. , Church Candice D. , James Eddie A. , Nghiem Paul , Kwok William W. , Koelle David M.
Published: April 2023

20mer peptides spanning the MCPyV T antigen space
Figure S5 from Human CD4+ T Cells Specific for Merkel Cell Polyomavirus Localize to Merkel Cell Carcinomas and Target a Required Oncogenic Domain
Authors: Longino Natalie V. , Yang Junbao , Iyer Jayasri G. , Ibrani Dafina , Chow I-Ting , Laing Kerry J. , Campbell Victoria L. , Paulson Kelly G. , Kulikauskas Rima M. , Church Candice D. , James Eddie A. , Nghiem Paul , Kwok William W. , Koelle David M.
Published: April 2023

Minimal epitope and cross reactivity determination of WEDLT209-228 -specific CD4 T cells clones.
Supplementary Figure S1 from Phase II Trial of Ipilimumab with Stereotactic Radiation Therapy for Metastatic Disease: Outcomes, Toxicities, and Low-Dose Radiation–Related Abscopal Responses
Authors: Welsh James W. , Tang Chad , de Groot Patricia , Naing Aung , Hess Kenneth R. , Heymach John V. , Papadimitrakopoulou Vassiliki A. , Cushman Taylor R. , Subbiah Vivek , Chang Joe Y. , Simon George R. , Ramapriyan Rishab , Barsoumian Hampartsoum B. , Menon Hari , Cortez Maria Angelica , Massarelli Erminia , Nguyen Quynh , Sharma Padmanee , Allison James P. , Diab Adi , Verma Vivek , Raju Uma , Shaaban Sherif G. , Dadu Ramona , Cabanillas Maria E. , Wang Kelvin , Anderson Clark , Gomez Daniel R. , Hahn Stephen , Komaki Ritsuko , Hong David S.
Published: April 2023

Supplementary Figure S1. CONSORT flow diagram for patient selection
Figure S4 from Mammary Tumor Cells with High Metastatic Potential Are Hypersensitive to Macrophage-Derived HGF
Authors: Kitamura Takanori , Kato Yu , Brownlie Demi , Soong Daniel Y.H. , Sugano Gaël , Kippen Nicolle , Li Jiufeng , Doughty-Shenton Dahlia , Carragher Neil , Pollard Jeffrey W.
Published: April 2023

Effects of MET signal on tumor cell apoptosis in vitro.
Figure S1 from Mammary Tumor Cells with High Metastatic Potential Are Hypersensitive to Macrophage-Derived HGF
Authors: Kitamura Takanori , Kato Yu , Brownlie Demi , Soong Daniel Y.H. , Sugano Gaël , Kippen Nicolle , Li Jiufeng , Doughty-Shenton Dahlia , Carragher Neil , Pollard Jeffrey W.
Published: April 2023

Microarray analysis to compare gene expression profile between E0771-parental and E0771-HML2 cells.
Supplementary Figures from GM-CSF Promotes Antitumor Immunity by Inducing Th9 Cell Responses
Authors: Kim Il-Kyu , Koh Choong-Hyun , Jeon Insu , Shin Kwang-Soo , Kang Tae-Seung , Bae Eun-Ah , Seo Hyungseok , Ko Hyun-Ja , Kim Byung-Seok , Chung Yeonseok , Kang Chang-Yuil
Published: April 2023

Supplementary Figs. 1-9
Supplementary Table S2 from Single-Cell Transcriptome Analysis Reveals Gene Signatures Associated with T-cell Persistence Following Adoptive Cell Therapy
Authors: Lu Yong-Chen , Jia Li , Zheng Zhili , Tran Eric , Robbins Paul F. , Rosenberg Steven A.
Published: April 2023

Supplementary Table S2
Movie 1 from Tumor Vessel Normalization, Immunostimulatory Reprogramming, and Improved Survival in Glioblastoma with Combined Inhibition of PD-1, Angiopoietin-2, and VEGF
Authors: Di Tacchio Mariangela , Macas Jadranka , Weissenberger Jakob , Sommer Kathleen , Bähr Oliver , Steinbach Joachim P. , Senft Christian , Seifert Volker , Glas Martin , Herrlinger Ulrich , Krex Dietmar , Meinhardt Matthias , Weyerbrock Astrid , Timmer Marco , Goldbrunner Roland , Deckert Martina , Scheel Andreas H. , Büttner Reinhard , Grauer Oliver M. , Schittenhelm Jens , Tabatabai Ghazaleh , Harter Patrick N. , Günther Stefan , Devraj Kavi , Plate Karl H. , Reiss Yvonne
Published: April 2023

Pericyte coverage in GL261 control tumors is shown (CollagenIV, red; desmin, green)
Supplementary Figures 1-8 and Tables 1-3 from A Fusion Protein Complex that Combines IL-12, IL-15, and IL-18 Signaling to Induce Memory-Like NK Cells for Cancer Immunotherapy
Authors: Becker-Hapak Michelle K. , Shrestha Niraj , McClain Ethan , Dee Michael J. , Chaturvedi Pallavi , Leclerc Gilles M. , Marsala Lynne I. , Foster Mark , Schappe Timothy , Tran Jennifer , Desai Sweta , Neal Carly C. , Pence Patrick , Wong Pamela , Wagner Julia A. , Russler-Germain David A. , Zhu Xiaoyun , Spanoudis Catherine M. , Gallo Victor L. , Echeverri Christian A. , Ramirez Laritza L. , You Lijing , Egan Jack O. , Rhode Peter R. , Jiao Jin-an , Muniz Gabriela J. , Jeng Emily K. , Prendes Caitlin A. , Sullivan Ryan P. , Berrien-Elliott Melissa M. , Wong Hing C. , Fehniger Todd A.
Published: April 2023

Supplemental Figures 1-8, Supplemental Tables 1-3
Supplementary Data from A DNA-Launched Nanoparticle Vaccine Elicits CD8+ T-cell Immunity to Promote In Vivo Tumor Control
Authors: Xu Ziyang , Chokkalingam Neethu , Tello-Ruiz Edgar , Wise Megan C. , Bah Mamadou A. , Walker Susanne , Tursi Nicholas J. , Fisher Paul D. , Schultheis Katherine , Broderick Kate E. , Humeau Laurent , Kulp Daniel W. , Weiner David B.
Published: April 2023

Supplementary figures 1-4 and Supplementary Table 1
Supplementary Figures from Tumor Cell–Intrinsic USP22 Suppresses Antitumor Immunity in Pancreatic Cancer
Authors: Li Jinyang , Yuan Salina , Norgard Robert J. , Yan Fangxue , Yamazoe Taiji , Blanco Andrés , Stanger Ben Z.
Published: April 2023

Supplementary Figures 1-4
Figures S1-S8, Table S1 from Idelalisib Rescues Natural Killer Cells from Monocyte-Induced Immunosuppression by Inhibiting NOX2-Derived Reactive Oxygen Species
Authors: Akhiani Ali A. , Hallner Alexander , Kiffin Roberta , Aydin Ebru , Werlenius Olle , Aurelius Johan , Martner Anna , Thorén Fredrik B. , Hellstrand Kristoffer
Published: April 2023

Supplementary Figures and Table
Supplementary Tables S1-S2 from IDO1 Signaling through GCN2 in a Subpopulation of Gr-1+ Cells Shifts the IFNγ/IL6 Balance to Promote Neovascularization
Authors: Dey Souvik , Mondal Arpita , DuHadaway James B. , Sutanto-Ward Erika , Laury-Kleintop Lisa D. , Thomas Sunil , Prendergast George C. , Mandik-Nayak Laura , Muller Alexander J.
Published: April 2023

Supplementary Tables S1-S2
Supplementary Figures from Activating Mucosal-Associated Invariant T Cells Induces a Broad Antitumor Response
Authors: Ruf Benjamin , Catania Vanessa V. , Wabitsch Simon , Ma Chi , Diggs Laurence P. , Zhang Qianfei , Heinrich Bernd , Subramanyam Varun , Cui Linda L. , Pouzolles Marie , Evans Christine N. , Chari Raj , Sakai Shunsuke , Oh Sangmi , Barry Clifton E. , Barber Daniel L. , Greten Tim F.
Published: April 2023

Supplementary Figure Legends, Figure S1 and Figure S2
Supplementary Figures from CD137/OX40 Bispecific Antibody Induces Potent Antitumor Activity that Is Dependent on Target Coengagement
Authors: Gaspar Miguel , Pravin John , Rodrigues Leonor , Uhlenbroich Sandra , Everett Katy L. , Wollerton Francisca , Morrow Michelle , Tuna Mihriban , Brewis Neil
Published: April 2023

Supplementary Figures 1-10
Table S4 from Characterization of BAY 1905254, an Immune Checkpoint Inhibitor Targeting the Immunoglobulin-Like Domain Containing Receptor 2 (ILDR2)
Authors: Huetter Julia , Gritzan Uwe , Gutcher Ilona , Doecke Wolf-Dietrich , Luetke-Eversloh Merlin V. , Golfier Sven , Roider Helge G. , Frisk Anna-Lena , Hunter John , Pow Andrew , Drake Andrew , Levine Zurit , Levy Ofer , Azulay Meir , Barbiro Inbal , Cojocaru Gady , Vaknin Ilan , Kreft Bertolt , Roese Lars
Published: April 2023

The amountof ILDR2 peptides in kidney and testis samples derived fromhuman, monkey and mouse.
Supplementary Table S3 from Pharmacologic Screening Identifies Metabolic Vulnerabilities of CD8+ T Cells
Authors: Drijvers Jefte M. , Gillis Jacob E. , Muijlwijk Tara , Nguyen Thao H. , Gaudiano Emily F. , Harris Isaac S. , LaFleur Martin W. , Ringel Alison E. , Yao Cong-Hui , Kurmi Kiran , Juneja Vikram R. , Trombley Justin D. , Haigis Marcia C. , Sharpe Arlene H.
Published: April 2023

Supplementary Table S3
Table S2 from Characterization of BAY 1905254, an Immune Checkpoint Inhibitor Targeting the Immunoglobulin-Like Domain Containing Receptor 2 (ILDR2)
Authors: Huetter Julia , Gritzan Uwe , Gutcher Ilona , Doecke Wolf-Dietrich , Luetke-Eversloh Merlin V. , Golfier Sven , Roider Helge G. , Frisk Anna-Lena , Hunter John , Pow Andrew , Drake Andrew , Levine Zurit , Levy Ofer , Azulay Meir , Barbiro Inbal , Cojocaru Gady , Vaknin Ilan , Kreft Bertolt , Roese Lars
Published: April 2023

Histopathological findings
Supplementary Table S1 from Combined BRAF, MEK, and CDK4/6 Inhibition Depletes Intratumoral Immune-Potentiating Myeloid Populations in Melanoma
Authors: Lelliott Emily J. , Mangiola Stefano , Ramsbottom Kelly M. , Zethoven Magnus , Lim Lydia , Lau Peter K.H. , Oliver Amanda J. , Martelotto Luciano G. , Kirby Laura , Martin Claire , Patel Riyaben P. , Slater Alison , Cullinane Carleen , Papenfuss Anthony T. , Haynes Nicole M. , McArthur Grant A. , Oliaro Jane , Sheppard Karen E.
Published: April 2023

Supplementary Table S1
Supplementary Figures from Combined BRAF, MEK, and CDK4/6 Inhibition Depletes Intratumoral Immune-Potentiating Myeloid Populations in Melanoma
Authors: Lelliott Emily J. , Mangiola Stefano , Ramsbottom Kelly M. , Zethoven Magnus , Lim Lydia , Lau Peter K.H. , Oliver Amanda J. , Martelotto Luciano G. , Kirby Laura , Martin Claire , Patel Riyaben P. , Slater Alison , Cullinane Carleen , Papenfuss Anthony T. , Haynes Nicole M. , McArthur Grant A. , Oliaro Jane , Sheppard Karen E.
Published: April 2023

Supplementary Figures S1-S5
Supplementary Tables S4-S10 from The Ratio of Exhausted to Resident Infiltrating Lymphocytes Is Prognostic for Colorectal Cancer Patient Outcome
Authors: Foroutan Momeneh , Molania Ramyar , Pfefferle Aline , Behrenbruch Corina , Scheer Sebastian , Kallies Axel , Speed Terence P. , Cursons Joseph , Huntington Nicholas D.
Published: April 2023

An excel file with several sheets, representing supp. tables S4-S10 in the paper.
Figure S7 from The Chemokine CX3CL1 Improves Trastuzumab Efficacy in HER2 Low–Expressing Cancer In Vitro and In Vivo
Authors: Dreyer Tobias F. , Kuhn Sabine , Stange Christoph , Heithorst Nadine , Schilling Daniela , Jelsma Jil , Sievert Wolfgang , Seitz Stefanie , Stangl Stefan , Hapfelmeier Alexander , Noske Aurelia , Wege Anja K. , Weichert Wilko , Ruland Jürgen , Schmitt Manfred , Dorn Julia , Kiechle Marion , Reuning Ute , Magdolen Viktor , Multhoff Gabriele , Bronger Holger
Published: April 2023

Impact of Cx3cl1 overexpression in the HT-29 xenograft model.
Figure S6 from The Chemokine CX3CL1 Improves Trastuzumab Efficacy in HER2 Low–Expressing Cancer In Vitro and In Vivo
Authors: Dreyer Tobias F. , Kuhn Sabine , Stange Christoph , Heithorst Nadine , Schilling Daniela , Jelsma Jil , Sievert Wolfgang , Seitz Stefanie , Stangl Stefan , Hapfelmeier Alexander , Noske Aurelia , Wege Anja K. , Weichert Wilko , Ruland Jürgen , Schmitt Manfred , Dorn Julia , Kiechle Marion , Reuning Ute , Magdolen Viktor , Multhoff Gabriele , Bronger Holger
Published: April 2023

CX3CL1 overexpression in HER2 overexpressing human breast cancer.
Figure S5 from The Chemokine CX3CL1 Improves Trastuzumab Efficacy in HER2 Low–Expressing Cancer In Vitro and In Vivo
Authors: Dreyer Tobias F. , Kuhn Sabine , Stange Christoph , Heithorst Nadine , Schilling Daniela , Jelsma Jil , Sievert Wolfgang , Seitz Stefanie , Stangl Stefan , Hapfelmeier Alexander , Noske Aurelia , Wege Anja K. , Weichert Wilko , Ruland Jürgen , Schmitt Manfred , Dorn Julia , Kiechle Marion , Reuning Ute , Magdolen Viktor , Multhoff Gabriele , Bronger Holger
Published: April 2023

Impact of Cx3cl1 overexpression in the MDA-MB 453 xenograft model.
Supplementary Figure S3 from TGFβ Programs Central Memory Differentiation in Ex Vivo–Stimulated Human T Cells
Authors: Dahmani Amina , Janelle Valérie , Carli Cédric , Richaud Manon , Lamarche Caroline , Khalili Myriam , Goupil Mathieu , Bezverbnaya Ksenia , Bramson Jonathan L. , Delisle Jean-Sébastien
Published: April 2023

Figure S3. Ex vivo stimulated Tem do not revert to Tcm
Supplementary Figure S2 from TGFβ Programs Central Memory Differentiation in Ex Vivo–Stimulated Human T Cells
Authors: Dahmani Amina , Janelle Valérie , Carli Cédric , Richaud Manon , Lamarche Caroline , Khalili Myriam , Goupil Mathieu , Bezverbnaya Ksenia , Bramson Jonathan L. , Delisle Jean-Sébastien
Published: April 2023

Additional Tcm markers
Supplemental Fig. S2 from Cross-talk between Colon Cells and Macrophages Increases ST6GALNAC1 and MUC1-sTn Expression in Ulcerative Colitis and Colitis-Associated Colon Cancer
Authors: Kvorjak Michael , Ahmed Yasmine , Miller Michelle L. , Sriram Raahul , Coronnello Claudia , Hashash Jana G. , Hartman Douglas J. , Telmer Cheryl A. , Miskov-Zivanov Natasa , Finn Olivera J. , Cascio Sandra
Published: April 2023

ST6GALNAC1 induces migratory properties of colon cancer cells.
Supplementary Data from Inhibition of MICA and MICB Shedding Elicits NK-Cell–Mediated Immunity against Tumors Resistant to Cytotoxic T Cells
Authors: Ferrari de Andrade Lucas , Kumar Sushil , Luoma Adrienne M. , Ito Yoshinaga , Alves da Silva Pedro Henrique , Pan Deng , Pyrdol Jason W. , Yoon Charles H. , Wucherpfennig Kai W.
Published: April 2023

Supplementary figures and table
Supplementary Movie S2 from Differential Expression of CD49a and CD49b Determines Localization and Function of Tumor-Infiltrating CD8+ T Cells
Authors: Melssen Marit M. , Lindsay Robin S. , Stasiak Katarzyna , Rodriguez Anthony B. , Briegel Amanda M. , Cyranowski Salwador , Rutkowski Melanie R. , Conaway Mark R. , Melief Cornelis J.M. , van der Burg Sjoerd H. , Eyo Ukpong , Slingluff Craig L. , Engelhard Victor H.
Published: April 2023

Movement of cells in anti-CD49b treated tumor slices
Supplementary Movie S1 from Differential Expression of CD49a and CD49b Determines Localization and Function of Tumor-Infiltrating CD8+ T Cells
Authors: Melssen Marit M. , Lindsay Robin S. , Stasiak Katarzyna , Rodriguez Anthony B. , Briegel Amanda M. , Cyranowski Salwador , Rutkowski Melanie R. , Conaway Mark R. , Melief Cornelis J.M. , van der Burg Sjoerd H. , Eyo Ukpong , Slingluff Craig L. , Engelhard Victor H.
Published: April 2023

Movement of cells in untreated tumor slices
Supplementary Data from CD40 Enhances Type I Interferon Responses Downstream of CD47 Blockade, Bridging Innate and Adaptive Immunity
Authors: de Silva Suresh , Fromm George , Shuptrine Casey W. , Johannes Kellsey , Patel Arpita , Yoo Kyung Jin , Huang Kaiwen , Schreiber Taylor H.
Published: April 2023

Supplementary Figures 1-6 and Supplementary Tables 1-3
Supplementary Tables and Figure Legends from A Bispecific Antibody Antagonizes Prosurvival CD40 Signaling and Promotes Vγ9Vδ2 T cell–Mediated Antitumor Responses in Human B-cell Malignancies
Authors: de Weerdt Iris , Lameris Roeland , Scheffer George L. , Vree Jana , de Boer Renate , Stam Anita G. , van de Ven Rieneke , Levin Mark-David , Pals Steven T. , Roovers Rob C. , Parren Paul W.H.I. , de Gruijl Tanja D. , Kater Arnon P. , van der Vliet Hans J.
Published: April 2023

Supplementary Tables 1-2 and Figure legends
Supplementary Table 1 from Nutlin-3a Enhances Natural Killer Cell–Mediated Killing of Neuroblastoma by Restoring p53-Dependent Expression of Ligands for NKG2D and DNAM-1 Receptors
Authors: Veneziani Irene , Infante Paola , Ferretti Elisa , Melaiu Ombretta , Battistelli Cecilia , Lucarini Valeria , Compagnone Mirco , Nicoletti Carmine , Castellano Aurora , Petrini Stefania , Ognibene Marzia , Pezzolo Annalisa , Di Marcotullio Lucia , Bei Roberto , Moretta Lorenzo , Pistoia Vito , Fruci Doriana , Barnaba Vincenzo , Locatelli Franco , Cifaldi Loredana
Published: April 2023

Sequences and schemes of PVR promoter regions, cloned into pGL3-basic vector by using BglII and HindIII restriction enzymes, to obtain PVR-promoter and PVR promoter p53-deleted plasmids used in luciferase reporter assay:
Supplementary Figures from Nutlin-3a Enhances Natural Killer Cell–Mediated Killing of Neuroblastoma by Restoring p53-Dependent Expression of Ligands for NKG2D and DNAM-1 Receptors
Authors: Veneziani Irene , Infante Paola , Ferretti Elisa , Melaiu Ombretta , Battistelli Cecilia , Lucarini Valeria , Compagnone Mirco , Nicoletti Carmine , Castellano Aurora , Petrini Stefania , Ognibene Marzia , Pezzolo Annalisa , Di Marcotullio Lucia , Bei Roberto , Moretta Lorenzo , Pistoia Vito , Fruci Doriana , Barnaba Vincenzo , Locatelli Franco , Cifaldi Loredana
Published: April 2023

Supplementary Figures
Figure S7 from Immunotargeting of the xCT Cystine/Glutamate Antiporter Potentiates the Efficacy of HER2-Targeted Immunotherapies in Breast Cancer
Authors: Conti Laura , Bolli Elisabetta , Di Lorenzo Antonino , Franceschi Valentina , Macchi Francesca , Riccardo Federica , Ruiu Roberto , Russo Luca , Quaglino Elena , Donofrio Gaetano , Cavallo Federica
Published: April 2023

Figure S7
Supplementary Legends from CD8+ T–cell Immune Surveillance against a Tumor Antigen Encoded by the Oncogenic Long Noncoding RNA PVT1
Authors: Kikuchi Yasuhiro , Tokita Serina , Hirama Tomomi , Kochin Vitaly , Nakatsugawa Munehide , Shinkawa Tomoyo , Hirohashi Yoshihiko , Tsukahara Tomohide , Hata Fumitake , Takemasa Ichiro , Sato Noriyuki , Kanaseki Takayuki , Torigoe Toshihiko
Published: April 2023

Supplementary Information
Supplementary Figure from Holistic Characterization of Tumor Monocyte-to-Macrophage Differentiation Integrates Distinct Immune Phenotypes in Kidney Cancer
Authors: Mujal Adriana M. , Combes Alexis J. , Rao Arjun A. , Binnewies Mikhail , Samad Bushra , Tsui Jessica , Boissonnas Alexandre , Pollack Joshua L. , Argüello Rafael J. , Meng Maxwell V. , Porten Sima P. , Ruhland Megan K. , Barry Kevin C. , Chan Vincent , Krummel Matthew F.
Published: April 2023

Supplementary Figure from Holistic Characterization of Tumor Monocyte-to-Macrophage Differentiation Integrates Distinct Immune Phenotypes in Kidney Cancer
Supplementary Data from Intravesical VAX014 Synergizes with PD-L1 Blockade to Enhance Local and Systemic Control of Bladder Cancer
Authors: Tsuji Shingo , Reil Katherine , Nelson Kinsey , Proclivo Veronica H. , McGuire Kathleen L. , Giacalone Matthew J.
Published: April 2023

Supplementary Data from Intravesical VAX014 Synergizes with PD-L1 Blockade to Enhance Local and Systemic Control of Bladder Cancer
Supplementary Figure from Adenosine-Deaminase-Acting-on-RNA-1 Facilitates T-cell Migration toward Human Melanoma Cells
Authors: Margolis Naama , Moalem Hanna , Meirson Tomer , Galore-Haskel Gilli , Markovits Ettai , Baruch Erez N. , Vizel Bella , Yeffet Avner , Kanterman-Rifman Julia , Debby Assaf , Besser Michal J. , Schachter Jacob , Markel Gal
Published: April 2023

Supplementary Figure from Adenosine-Deaminase-Acting-on-RNA-1 Facilitates T-cell Migration toward Human Melanoma Cells
Supplementary Data from Long Noncoding RNA MIR4435-2HG Suppresses Colorectal Cancer Initiation and Progression By Reprogramming Neutrophils
Authors: Yu Hongfei , Chen Chaoyi , Han Fengyan , Tang Jinlong , Deng Mengli , Niu Yumiao , Lai Maode , Zhang Honghe
Published: April 2023

Supplementary Data from Long Noncoding RNA MIR4435-2HG Suppresses Colorectal Cancer Initiation and Progression By Reprogramming Neutrophils
Supplementary Data from Sensory Nerves Impede the Formation of Tertiary Lymphoid Structures and Development of Protective Antimelanoma Immune Responses
Authors: Vats Kavita , Kruglov Oleg , Sahoo Bikram , Soman Vishal , Zhang Jiying , Shurin Galina V. , Chandran Uma R. , Skums Pavel , Shurin Michael R. , Zelikovsky Alex , Storkus Walter J. , Bunimovich Yuri L.
Published: April 2023

Supplementary Data from Sensory Nerves Impede the Formation of Tertiary Lymphoid Structures and Development of Protective Antimelanoma Immune Responses
Supplementary Figure from CD8+ T-cell Responses Are Boosted by Dual PD-1/VEGFR2 Blockade after EGFR Inhibition in Egfr-Mutant Lung Cancer
Authors: Nishii Kazuya , Ohashi Kadoaki , Tomida Shuta , Nakasuka Takamasa , Hirabae Atsuko , Okawa Sachi , Nishimura Jun , Higo Hisao , Watanabe Hiromi , Kano Hirohisa , Ando Chihiro , Makimoto Go , Ninomiya Kiichiro , Kato Yuka , Kubo Toshio , Ichihara Eiki , Hotta Katsuyuki , Tabata Masahiro , Toyooka Shinichi , Udono Heiichiro , Maeda Yoshinobu , Kiura Katsuyuki
Published: April 2023

Supplementary Figure from CD8+ T-cell Responses Are Boosted by Dual PD-1/VEGFR2 Blockade after EGFR Inhibition in Egfr-Mutant Lung Cancer
Supplementary Figure from Immune Phenotypes and Target Antigens of Clonally Expanded Bone Marrow T Cells in Treatment-Naïve Multiple Myeloma
Authors: Welters Carlotta , Lammoglia Cobo María Fernanda , Stein Christian Alexander , Hsu Meng-Tung , Ben Hamza Amin , Penter Livius , Chen Xiaojing , Buccitelli Christopher , Popp Oliver , Mertins Philipp , Dietze Kerstin , Bullinger Lars , Moosmann Andreas , Blanc Eric , Beule Dieter , Gerbitz Armin , Strobel Julian , Hackstein Holger , Rahn Hans-Peter , Dornmair Klaus , Blankenstein Thomas , Hansmann Leo
Published: April 2023

Supplementary Figure from Immune Phenotypes and Target Antigens of Clonally Expanded Bone Marrow T Cells in Treatment-Naïve Multiple Myeloma
Table S6-8 from Extracellular Vesicle–Packaged circATP2B4 Mediates M2 Macrophage Polarization via miR-532-3p/SREBF1 Axis to Promote Epithelial Ovarian Cancer Metastasis
Authors: Wang Fang , Niu Yuequn , Chen Kelie , Yuan Xiaoyu , Qin Yuheng , Zheng Fang , Cui Zhenyan , Lu Weiguo , Wu Yihua , Xia Dajing
Published: April 2023

Supplementary table S6-8
Figure S3 from Extracellular Vesicle–Packaged circATP2B4 Mediates M2 Macrophage Polarization via miR-532-3p/SREBF1 Axis to Promote Epithelial Ovarian Cancer Metastasis
Authors: Wang Fang , Niu Yuequn , Chen Kelie , Yuan Xiaoyu , Qin Yuheng , Zheng Fang , Cui Zhenyan , Lu Weiguo , Wu Yihua , Xia Dajing
Published: April 2023

Supplementary Figure S3
Supplementary Table S2 from Infiltration of Tumors Is Regulated by T cell–Intrinsic Nitric Oxide Synthesis
Authors: Cunha Pedro P. , Bargiela David , Minogue Eleanor , Krause Lena C.M. , Barbieri Laura , Brombach Carolin , Gojkovic Milos , Marklund Emilia , Pietsch Sandra , Foskolou Iosifina , Branco Cristina M. , Veliça Pedro , Johnson Randall S.
Published: April 2023

Supplementary Table S2
Supplementary Data from CD59-Regulated Ras Compartmentalization Orchestrates Antitumor T-cell Immunity
Authors: Li Luying , Ding Peipei , Lv Xinyue , Xie Suhong , Li Ling , Chen Jianfeng , Zhou Danlei , Wang Xiaochao , Wang Qi , Zhang Wei , Xu Yanqing , Lu Renquan , Hu Weiguo
Published: April 2023

Supplementary Data from CD59-Regulated Ras Compartmentalization Orchestrates Antitumor T-cell Immunity
Supplementary Figures and Tables from A Novel Engineered AAV-Based Neoantigen Vaccine in Combination with Radiotherapy Eradicates Tumors
Authors: Huang Kevin Chih-Yang , Lai Chia-Ying , Hung Wei-Ze , Chang Hsin-Yu , Lin Pei-Chun , Chiang Shu-Fen , Ke Tao-Wei , Liang Ji-An , Shiau An-Cheng , Yang Pei-Chen , Chen William Tzu-Liang , Chao K.S. Clifford
Published: April 2023

Supplementary Figures S1-S6 and Tables S1-S3
Data from CD16+NKG2Ahigh Natural Killer Cells Infiltrate Breast Cancer–Draining Lymph Nodes
Authors: Frazao Alexandra , Messaoudene Meriem , Nunez Nicolas , Dulphy Nicolas , Roussin France , Sedlik Christine , Zitvogel Laurence , Piaggio Eliane , Toubert Antoine , Caignard Anne
Published: April 2023

Abstract
Tumor-draining lymph nodes (TD-LNs) are the first site of metastasis of breast cancer. Natural killer (NK) cells that infiltrate TD-LNs [including noninvaded (NI) or metastatic (M)-LNs from breast cancer patients] and NK cells from healthy donor (HD)-LNs were characterized, and their phenotype analyzed by flow cytometry. Low percentages of tumor cells invaded M-LNs, and these cells expressed ULBP2 and HLA class I molecules. Although NK cells from paired NI and M-LNs were similar, they expressed different markers compared with HD-LN NK cells. Compared with HD-LNs, TD-LN NK cells expressed activating DNAM-1, NKG2C and inhibitory NKG2A receptors, and exhibited elevated CXCR3 expression. CD16, NKG2A, and NKp46 expression were shown to be increased in stage IIIA breast cancer patients. TD-LNs contained a large proportion of activated CD56brightCD16+ NK cells with high expression of NKG2A. We also showed that a subset of LN NK cells expressed PD-1, expression of which was correlated with NKp30 and NKG2C expression. LN NK cell activation status was evaluated by degranulation potential and lytic capacity toward breast cancer cells. NK cells from TD-LNs degranulated after coculture with breast cancer cell lines. Cytokine-activated TD-LN NK cells exerted greater lysis of breast cancer cell lines than HD-LN NK cells and preferentially lysed the HLA class Ilow MCF-7 breast cancer cell line. TD-LNs from breast cancer patients, thus, contained activated lytic NK cells. The expression of inhibitory receptor NKG2A and checkpoint PD-1 by NK cells infiltrating breast cancer–draining LNs supports their potential as targets for immunotherapies using anti-NKG2A and/or anti–PD-1.
Data from Phenotypic and Genomic Determinants of Immunotherapy Response Associated with Squamousness
Authors: Goodman Aaron M. , Kato Shumei , Chattopadhyay Ranajoy , Okamura Ryosuke , Saunders Ila M. , Montesion Meagan , Frampton Garrett M. , Miller Vincent A. , Daniels Gregory A. , Kurzrock Razelle
Published: April 2023

Abstract
Advanced and metastatic squamous cell carcinomas (SCC) are common and difficult-to-treat malignancies. We assessed 75 immunotherapy-treated patients with SCC from a clinically annotated database of 2,651 patients, as well as 9,407 patients from a deidentified database for molecular features that might influence checkpoint blockade response. SCCs had higher tumor mutational burdens (TMB) than non-SCCs (P < 0.0001). Cutaneous SCCs had the highest TMB (P < 0.0001), with 41.3% demonstrating a very high TMB (≥50 mutations/Mb). In immunotherapy-treated patients with SCC, higher TMB (≥12 mutations/Mb) correlated with a trend to higher clinical benefit rate [stable disease ≥ 6 months or partial/complete remission; 60% vs. 29%; (high vs. low TMB); P = 0.06] and significantly longer median time-to-treatment failure (TTF; 9.9 vs. 4.4 months; P = 0.0058). Cutaneous SCCs had the highest clinical benefit [11/15 patients (73%) vs. 20/60 (33%) non-cutaneous (P = 0.008)], TTF (P = 0.0015), and overall survival (P = 0.06) with immunotherapy treatment. In conclusion, among a diverse set of SCCs, higher TMB and cutaneous disease associated with better immunotherapy outcome.
Supplementary Data from Modulation of CD22 Protein Expression in Childhood Leukemia by Pervasive Splicing Aberrations: Implications for CD22-Directed Immunotherapies
Authors: Zheng Sisi , Gillespie Elisabeth , Naqvi Ammar S. , Hayer Katharina E. , Ang Zhiwei , Torres-Diz Manuel , Quesnel-Vallières Mathieu , Hottman David A. , Bagashev Asen , Chukinas John , Schmidt Carolin , Asnani Mukta , Shraim Rawan , Taylor Deanne M. , Rheingold Susan R. , O'Brien Maureen M. , Singh Nathan , Lynch Kristen W. , Ruella Marco , Barash Yoseph , Tasian Sarah K. , Thomas-Tikhonenko Andrei
Published: April 2023

Supplementary Data from Modulation of CD22 Protein Expression in Childhood Leukemia by Pervasive Splicing Aberrations: Implications for CD22-Directed Immunotherapies
Table S5 from Type-I Interferon Signaling Is Essential for Robust Metronomic Chemo-Immunogenic Tumor Regression in Murine Breast Cancer
Authors: Vergato Cameron , Doshi Kshama A. , Roblyer Darren , Waxman David J.
Published: April 2023

Table S5. RNAseq analysis of E0771 tumors, with vs without CPA treatment.
Revision of the Lucanus boileaui group and notes on L . takeoi Adachi, 2020 from the L . maculifemoratus group (Coleoptera: Lucanidae: Lucaninae)
Authors: QI ZHI-HAO , HE HAO , SU RONG-XIANG , AI HONG-MU , SONG HAI-TIAN
Published: April 2023

A new stag beetle is described from Yunnan Province, China: Lucanus jiaozishanus Qi, He, Su & Song, new species. It is the third species of the L. boileaui group which are distributed only in Southwestern China and Northeastern Myanmar. The differences of the new species with the more related taxa (L. boileaui Planet, 1897, L. ludivinae Boucher, 1998 and L. takeoi Adachi, 2020) are discussed. A new synonymy, L. bidentis Schenk, 2013 = L. boileaui, is proposed. Keys to males and females of the L. boileaui group are provided. The variation of males, the female and the host plant of L. takeoi (L. maculifemoratus group) are illustrated for the first time.
Using Neural Networks to Learn the Jet Stream Forced Response from Natural Variability
Authors: Connolly Charlotte , Barnes Elizabeth A. , Hassanzadeh Pedram , Pritchard Mike
Published: April 2023

Two distinct features of anthropogenic climate change, warming in the tropical upper troposphere and warming at the Arctic surface, have competing effects on the midlatitude jet stream’s latitudinal position, often referred to as a “tug-of-war.” Studies that investigate the jet’s response to these thermal forcings show that it is sensitive to model type, season, initial atmospheric conditions, and the shape and magnitude of the forcing. Much of this past work focuses on studying a simulation’s response to external manipulation. In contrast, we explore the potential to train a convolutional neural network (CNN) on internal variability alone and then use it to examine possible nonlinear responses of the jet to tropospheric thermal forcing that more closely resemble anthropogenic climate change. Our approach leverages the idea behind the fluctuation–dissipation theorem, which relates the internal variability of a system to its forced response but so far has been only used to quantify linear responses. We train a CNN on data from a long control run of the CESM dry dynamical core and show that it is able to skillfully predict the nonlinear response of the jet to sustained external forcing. The trained CNN provides a quick method for exploring the jet stream sensitivity to a wide range of tropospheric temperature tendencies and, considering that this method can likely be applied to any model with a long control run, could be useful for early-stage experiment design.
WORDS MATTER
Authors: Collins Stephen , DeWitt Jeff
Published: April 2023

Twitter is regarded today as an essential communication platform of U.S. diplomacy. Of all diplomatic tweets, those published by U.S. presidents carry the greatest weight and hold great potential to influence perceptions of the country. In this study, we conduct cross‐presidential comparative analyses on an original dataset of over 2,000 tweets published by the first two presidents of the Twitter era. In particular, we test the commonly held notion that the substance and tone of Barack Obama's communication reflected positively on America's image abroad, with the potential to expand soft power—a vital foreign policy asset—while Donald Trump's communication reflected negatively on America's image, potentially eroding the nation's image and its soft power. Findings demonstrate that what and how presidents communicate on Twitter may produce profound and disparate impacts on America's image abroad and on U.S. soft power.
Effect of plant growth regulators DA-6 and COS on drought tolerance of pineapple through bromelain and oxidative stress
Authors: Huang XiaoKui , Rao GangShun , Peng XiaoDu , Xue YingBin , Hu HanQiao , Feng NaiJie , Zheng DianFeng
Published: April 2023

Background

Due to global warming, drought climates frequently occur on land, and despite being drought resistant, pineapples are still subjected to varying degrees of drought stress. Plant growth regulators can regulate the stress tolerance of plants through hormonal effects. This experiment aims to investigate the regulatory effects of different plant growth regulators on Tainong- 16 and MD-2 Pineapple when subjected to drought stress.

Results

In this experiment, we examined the regulatory effects of two different plant growth regulators, sprayed on two pineapple varieties: MD-2 Pineapple and Tainong-16. The main component of T1 was diethyl aminoethyl hexanoate (DA-6) and that of T2 is chitosan oligosaccharide (COS). An environment similar to a natural drought was simulated in the drought stress treatments. Then, pineapples at different periods were sampled and a series of indicators were measured. The experimental results showed that the drought treatments treated with T1 and T2 plant growth regulators had a decrease in malondialdehyde, an increase in bromelain and antioxidant enzyme indicators, and an increase in phenotypic and yield indicators.

Conclusion

This experiment demonstrated that DA-6 and COS can enhance the drought resistance of pineapple plants to a certain extent through bromelain and oxidative stress. Therefore, DA-6 and COS have potential applications and this experiment lays the foundation for further research.
Early symptoms preceding post-infectious irritable bowel syndrome following COVID-19: a retrospective observational study incorporating daily gastrointestinal symptoms
Authors: Yamamoto Ryo , Yamamoto Asako , Masaoka Tatsuhiro , Homma Koichiro , Matsuoka Tadashi , Takemura Ryo , Wada Michihiko , Sasaki Junichi ,   Keio Donner Project, Kanai Takanori , Amagai Masayuki , Saya Hideyuki , Nishihara Hiroshi
Published: April 2023

Background

Intestinal microinflammation with immune dysfunction due to severe acute respiratory syndrome coronavirus 2 reportedly precipitates post-infectious irritable bowel syndrome. This study aimed to elucidate potential risk factors for subsequent development of irritable bowel syndrome, hypothesizing that it is associated with specific symptoms or patient backgrounds.

Methods

This single-center retrospective observational study (2020–2021) included adults with confirmed coronavirus disease requiring hospital admission and was conducted using real-world data retrieved from a hospital information system. Patient characteristics and detailed gastrointestinal symptoms were obtained and compared between patients with and without coronavirus disease-induced irritable bowel syndrome. Multivariate logistic models were used to validate the risk of developing irritable bowel syndrome. Moreover, daily gastrointestinal symptoms during hospitalization were examined in patients with irritable bowel syndrome.

Results

Among the 571 eligible patients, 12 (2.1%) were diagnosed with irritable bowel syndrome following coronavirus disease. While nausea and diarrhea during hospitalization, elevated white blood cell count on admission, and intensive care unit admission were associated with the development of irritable bowel syndrome, nausea and diarrhea were identified as risk factors for its development following coronavirus disease, as revealed by the adjusted analyses (odds ratio, 4.00 [1.01–15.84] and 5.64 [1.21–26.31], respectively). Half of the patients with irritable bowel syndrome had both diarrhea and constipation until discharge, and constipation was frequently followed by diarrhea.

Conclusions

While irritable bowel syndrome was rarely diagnosed following coronavirus disease, nausea and diarrhea during hospitalization precede the early signs of irritable bowel syndrome following coronavirus disease.
Interrelationship between muscle fitness in childhood and bone mineral density in adulthood: mediation analysis of muscle fitness in adulthood
Authors: Barbosa Cynthia Correa Lopes , da Costa Julio Cesar , Romanzini Catiana Leila Possamai , Batista Mariana Biagi , Blasquez-Shigaki Gabriela , Fernandes Rômulo Araújo , Martinho Diogo V. , Oliveira Tomás , Ribeiro Luís P. , Coelho-e-Silva Manuel João , Ronque Enio Ricardo Vaz
Published: April 2023

Background

This study was aimed to examine the relationship between muscular fitness indicators in childhood and areal bone mineral density (aBMD) in adulthood and to verify whether the relationship is mediated by performance on muscular fitness indicators in adulthood.

Methods

A sample of 138 healthy adults (69 males; 22.3 years) were followed after a previous assessment at the age of 7–10 years. Stature, body mass and muscular fitness indicators (handgrip strength, standing long jump and sit-ups tests) were assessed in childhood and adulthood. Additionally, total body, upper limbs, lower limbs, right femoral neck and lumbar spine aBMD was assessed in adulthood using dual X-ray absorptiometry. Analysis included descriptive statistics; t-test or Mann-Whitney U-test for comparison between males and females, multiple linear regression for the prediction aBMD from muscular fitness indicators in childhood, mediation analysis of the respective muscular fitness indicators in adulthood and the relationship between muscular fitness indicators in childhood and aBMD.

Results

Males were stronger compared to females regarding muscular fitness indicators in childhood and adulthood, and presented higher mean values for aBMD in adulthood, except for lumbar spine (p < 0.05). Regression analysis revealed that some muscular fitness indicators in childhood showed significant positive relationship with bone health indicators in adulthood, such as: handgrip strength and total body aBMD (β = 0.005; R2 = 0.35; p = 0.040) and upper limbs aBMD (β = 0.005; R2 = 0.55; p = 0.019); and sit-ups test was a significant predictors of lumbar spine BMD (β = 0.003; R2 = 0.06; p = 0.039). Mediation analysis pointed out the following: adulthood handgrip strength mediated relationships between childhood handgrip strength and total aBMD (indirect effect (IE) = 0.0025; 95%CI = 0.0005–0.0048), and upper limbs aBMD (IE = 0.0040; 95%CI = 0.0017–0.0069).

Conclusions

Muscular fitness indicators in childhood showed significant relationship with bone health indicators in adulthood and the sit-ups test in childhood had direct effect on lumbar spine aBMD in adulthood. Adulthood handgrip strength mediated the relationship between childhood handgrip strength and total body and upper limb aBMD, pointing out that muscular fitness in childhood may be a aBMD determinant in adulthood, especially when higher muscle fitness performance is maintained in adulthood.
Pulmonary function in a cohort of heart-healthy individuals from Northern Sweden—a comparison with discordant reference values
Authors: Erelund Sofia , Karp Kjell , Arvidsson Sandra , Johansson Bengt , Sundström Nina , Wiklund Urban
Published: April 2023

Background

Dynamic spirometry is an important investigation to differentiate between impaired and normal lung function. This study aimed to evaluate the results of lung function testing in a cohort of subjects from Northern Sweden without any known heart or pulmonary disease. Our focus was to compare with two reference materials that have showed differences in the age-dependency of lung function in Swedish subjects.

Methods

The study population consisted of 285 healthy adults (148 males, 52%) between 20–90 years of age. The subjects had been randomly selected from the population register for inclusion in a study investigating cardiac function in heart-healthy subjects, but were also assessed with dynamic spirometry. At least seven percent reported smoking. Sixteen subjects presented with pulmonary functional impairments and were excluded from the current study. The sex-specific age-dependency in lung volumes was estimated using the LMS model, where non-linear equations were derived for the mean value (M), the location (L) or skewness, and the scatter (S) or coefficient of variation. This model of the observed lung function data was compared with reference values given by the original LMS model published by the Global Lung Initiative (GLI), and with the model from the recent Obstructive Lung Disease In Norrbotten (OLIN) study, where higher reference values were presented for Swedish subjects than those given by the GLI model.

Results

No differences were found in the age-dependency of pulmonary function between the LMS model developed in the study and the OLIN model. Although the study group included smokers, the original GLI reference values suggested significantly lower normal values of FEV1 (forced expiratory volume) and FVC (forced vital capacity), and consequently fewer subjects below the lower limit of normality, than both the rederived LMS and OLIN models.

Conclusions

Our results are in line with previous reports and support that the original GLI reference values underestimate pulmonary function in the adult Swedish population. This underestimation could be reduced by updating the coefficients in the underlying LMS model based on a larger cohort of Swedish citizens than was available in this study.