bluuebunny/crossref_metadata_2025 huggingface dataset:
Insights into Nanoscale Wettability Effects of Low Salinity and Nanofluid Enhanced Oil Recovery Techniques
Authors: Afekare Dayo , Garno Jayne C. , Rao Dandina
Published: August 2020

In this study, enhanced oil recovery (EOR) techniques—namely low salinity and nanofluid EOR—are probed at the nanometer-scale using an atomic force microscope (AFM). Mica substrates were used as model clay-rich rocks while AFM tips were coated to present alkyl (-CH3), aromatic (-C6H5) and carboxylic acid (-COOH) functional groups, to simulate oil media. We prepared brine formulations to test brine dilution and cation bridging effects while selected concentrations (0 to 1 wt%) of hydrophilic SiO2 nanoparticles dispersed in 1 wt% NaCl were used as nanofluids. Samples were immersed in fluid cells and chemical force mapping was used to measure the adhesion force between polar/non-polar moieties to substrates. Adhesion work was evaluated based on force-displacement curves and compared with theories. Results from AFM studies indicate that low salinity waters and nanoparticle dispersions promote nanoscale wettability alteration by significantly reducing three-phase adhesion force and the reversible thermodynamic work of adhesion, also known as adhesion energy. The maximum reduction in adhesion energy obtained in experiments was in excellent agreement with existing theories. Electrostatic repulsion and reduced non-electrostatic adhesion are prominent surface forces common to both low salinity and nanofluid EOR. Structural forces are complex in nature and may not always decrease total adhesion force and energy at high nanoparticle concentration. Wettability effects also depend on surface chemical groups and the presence of divalent Mg2+ and Ca2+ cations. This study provides fresh insights and fundamental information about low salinity and nanofluid EOR while demonstrating the application of force-distance spectroscopy in investigating EOR techniques.
Effects of Progressive Resistance Training on Cognition and IGF-1 Levels in Elder Women Who Live in Areas with High Air Pollution
Authors: Molina-Sotomayor Edgardo , Castillo-Quezada Humberto , Martínez-Salazar Cristian , González-Orb Marcelo , Espinoza-Salinas Alexis , Gonzalez-Jurado Jose Antonio
Published: August 2020

The aim of this study was to determine the effects of a muscular strength programme on the levels of insulin-like growth factor-1 (IGF-1) and cognitive status in elder women with mild cognitive impairment who lived in areas of high air pollution. A total of 157 women participated in the study, distributed in four groups: Active/Clean (AC n = 38) and Active/Pollution (AP n = 37), who carried out a progressive resistance training programme for 24 months, and Sedentary/Clean (SC n = 40) and Sedentary/Pollution (SP n = 42). Maximum strength in the upper and lower limbs (1RM), cognition (Mini-Mental Scale Examination (MMSE)) and blood IGF-1 were evaluated. At the beginning of the intervention, there were no differences between the groups in the assessed variables. The active groups which carried out the resistance training programme (AC and AP), registered better results in IGF-1 than the sedentary groups. These differences were statistically significant in AC vs. SC (p < 0.01) and AP vs. SC (p < 0.05). Regarding MMSE, group AC registered the highest score increases (+8.2%) (significantly better than the other groups), while group SP worsened (−7%) significantly compared to the other three groups. In conclusion, resistance training had a positive effect on IGF-1, while sedentary behaviour and air pollution had a negative effect on cognitive status.
Source-Specific Volatile Organic Compounds and Emergency Hospital Admissions for Cardiorespiratory Diseases
Authors: Ran Jinjun , Kioumourtzoglou Marianthi-Anna , Sun Shengzhi , Han Lefei , Zhao Shi , Zhu Wei , Li Jinhui , Tian Linwei
Published: August 2020

Knowledge gaps remain regarding the cardiorespiratory impacts of ambient volatile organic compounds (VOCs) for the general population. This study identified contributing sources to ambient VOCs and estimated the short-term effects of VOC apportioned sources on daily emergency hospital admissions for cardiorespiratory diseases in Hong Kong from 2011 to 2014. We estimated VOC source contributions using fourteen organic chemicals by positive matrix factorization. Then, we examined the associations between the short-term exposure to VOC apportioned sources and emergency hospital admissions for cause-specific cardiorespiratory diseases using generalized additive models with polynomial distributed lag models while controlling for meteorological and co-pollutant confounders. We identified six VOC sources: gasoline emissions, liquefied petroleum gas (LPG) usage, aged VOCs, architectural paints, household products, and biogenic emissions. We found that increased emergency hospital admissions for chronic obstructive pulmonary disease were positively linked to ambient VOCs from gasoline emissions (excess risk (ER%): 2.1%; 95% CI: 0.9% to 3.4%), architectural paints (ER%: 1.5%; 95% CI: 0.2% to 2.9%), and household products (ER%: 1.5%; 95% CI: 0.2% to 2.8%), but negatively associated with biogenic VOCs (ER%: −6.6%; 95% CI: −10.4% to −2.5%). Increased congestive heart failure admissions were positively related to VOCs from architectural paints and household products in cold seasons. This study suggested that source-specific VOCs might trigger the exacerbation of cardiorespiratory diseases.
Impact of Air Pollution on Asthma Outcomes
Authors: Tiotiu Angelica I. , Novakova Plamena , Nedeva Denislava , Chong-Neto Herberto Jose , Novakova Silviya , Steiropoulos Paschalis , Kowal Krzysztof
Published: August 2020

Asthma is a chronic respiratory disease characterized by variable airflow obstruction, bronchial hyperresponsiveness, and airway inflammation. Evidence suggests that air pollution has a negative impact on asthma outcomes in both adult and pediatric populations. The aim of this review is to summarize the current knowledge on the effect of various outdoor and indoor pollutants on asthma outcomes, their burden on its management, as well as to highlight the measures that could result in improved asthma outcomes. Traffic-related air pollution, nitrogen dioxide and second-hand smoking (SHS) exposures represent significant risk factors for asthma development in children. Nevertheless, a causal relation between air pollution and development of adult asthma is not clearly established. Exposure to outdoor pollutants can induce asthma symptoms, exacerbations and decreases in lung function. Active tobacco smoking is associated with poorer asthma control, while exposure to SHS increases the risk of asthma exacerbations, respiratory symptoms and healthcare utilization. Other indoor pollutants such as heating sources and molds can also negatively impact the course of asthma. Global measures, that aim to reduce exposure to air pollutants, are highly needed in order to improve the outcomes and management of adult and pediatric asthma in addition to the existing guidelines.
Trends and Predictors for the Uptake of Colon Cancer Screening Using the Fecal Occult Blood Test in Spain from 2011 to 2017
Authors: Zamorano-Leon José Javier , López-de-Andres Ana , Álvarez-González Ana , Maestre-Miquel Clara , Astasio-Arbiza Paloma , López-Farré Antonio , de-Miguel-Diez Javier , Jiménez-García Rodrigo , Albaladejo-Vicente Romana
Published: August 2020

Background: In Spain, colorectal cancer screening using the fecal occult blood test, targeted towards the 50–69 age bracket, was implemented on different dates. We aim to assess the temporal trend of colorectal cancer (CRC) screening uptake according to the year of screening implementation in each region and to identify predictors for the uptake of CRC screening. Methods: A cross-sectional study with 12,657 participants from the Spanish National Health Surveys 2011 and 2017 was used. Uptake rates were analyzed according to the date that the screening program was implemented. Results: For regions with programs implemented before 2011, the uptake rate increased 3.34-fold from 2011 to 2017 (9.8% vs. 32.7%; p < 0.001). For regions that implemented screening within the 2011–2016 period, the uptake rose from 4.3% to 13.2% (3.07-fold; p < 0.001), and for regions that implemented screening after 2016, the uptake increased from 3.4% to 8.8% (2.59-fold; p < 0.001). For the entire Spanish population, the uptake increased 3.21-fold (6.8% vs. 21.8%; p < 0.001). Positive predictors for uptake were older age, Spanish nationality, middle-to-high educational level, suffering chronic diseases, non-smoking and living in regions where screening programs were implemented earlier. Conclusions: The different periods for the implementation of CRC screening as well as sociodemographic and health inequalities may have limited the improvement in the screening uptake from 2011 to 2017 in Spain.
Personality as a Resource for Labor Market Participation among Individuals with Chronic Health Conditions
Authors: Brouwer Sandra , van Zon Sander K. R. , Bültmann Ute , Riese Harriëtte , Jeronimus Bertus F.
Published: August 2020

Background: The link between personality traits and employment status in individuals with chronic health conditions (CHCs) is largely unexplored. In this study, we examined this association among 21,173 individuals with CHCs and whether this association differs between individuals suffering from a heart disease, depression, anxiety, cancer, chronic obstructive pulmonary disease, musculoskeletal disease (MSD) and type 2 diabetes mellitus (T2DM). Methods: This study was conducted using baseline data from the Lifelines Cohort Study. Employment status and the presence of CHCs were determined by questionnaire data. The Revised Neuroticism-Extroversion-Openness Personality Inventory (NEO-PI-R) was used to measure eight personality facet traits. We conducted disease-generic and disease-specific logistic regression analyses. Results: Workers with higher scores on self-consciousness (OR: 1.02; 95% CI: 1.01–1.02), impulsivity (1.03; 1.02–1.04), excitement seeking (1.02; 1.01–1.02), competence (1.08; 1.07–1.10) and self-discipline (1.04; 1.03–1.05) were more often employed. Adults with higher scores on anger-hostility (0.97; 0.97–0.98), vulnerability (0.98; 0.97–0.99), and deliberation (0.96; 0.95–0.97) were least often employed. Personality facets were associated strongest with employment status among individuals suffering from MSD and weakest in individuals with T2DM. Conclusions: Personality might be a key resource to continue working despite having a CHC. This may be relevant for the development of targeted personality-focused interventions.
Assessing the Safety Risks of Civil Engineering Laboratories Based on Lab Criticity Index: A Case Study in Jiangsu Province
Authors: Zhang Yi , Mao Peng , Li Hongyang , Xu Yuxin , You Dan , Liu Hui , Huang Wei , Yuan Jingfeng
Published: August 2020

With the rapid development of the construction industry, an increasing amount of attention were paid by universities to the development of civil engineering experiment courses so as to improve the practical research abilities of students. In recent years, due to the frequent occurrence of civil engineering laboratory accidents, it has become an urgent issue regarding on what factors influencing safety risks and how to assess and reduce the safety risks in civil engineering laboratories. Based on the lab criticity index (LCI) model, the research specificities of civil engineering laboratories were analyzed through literature review and expert interviews and 13 risk factors of civil engineering laboratories, from the four aspects of man, object, management, and environment, identified. The data for each parameter in the LCI model was obtained through a questionnaire survey, and finally the LCI value was calculated to evaluate priority. Among them, insufficient safety awareness of operators, danger due to equipment failure, imperfect management policies, and complex floor conditions were listed as the most common risk factors. Based on the LCI model, the worsening factors of these four risk factors were further analyzed. The LCI model is applied to the new research field of safety risk assessment in civil engineering laboratories that few researchers have studied before and a risk list for civil engineering laboratories was created. We revealed the safety status of civil engineering laboratories in Jiangsu Province and provided feasible suggestions for improving the management and supervision of civil engineering laboratories at universities. It can strengthen operator awareness of the risks in civil engineering laboratories and improve the social group’s attention to the safety risks of the laboratories, thus reducing the accidents’ possibility and seriousness of civil engineering laboratories.
Are Barriers the Same Whether I Want to Start or Maintain Exercise? A Narrative Review on Healthy Older Adults
Authors: André Nathalie , Agbangla Nounagnon Frutueux
Published: August 2020

To help older adults begin or adhere to regular physical exercise, several studies have endeavored to identify barriers to active behavior. However, there is a lack of information about barriers for active older people. In addition, most of the reviews of the literature compare only active people to inactive or sedentary people without examining in detail the barriers with respect to the degree of commitment to behavioral change. Finally, there is no consistency in the results of studies investigating the effects of barriers on the relationship between stages of change and exercise behavior. The first aim of this narrative review is to compare barriers that affect exercise stages of change from those that affect levels of exercise behavior in a healthy older population and the factors that can lead to relapse or dropout; the second aim is to identify the extent to which barriers hinder the relationships between stages of change and exercise behaviors. The results showed that barriers are well identified in sedentary people and in the first two stages of change (pre-contemplation and contemplation) compared to active seniors and other stages of change (preparation, action and maintenance). Consistency between the formulations of the different stages in comparison with the transtheoretical model and the definition of barriers and the limitations of measuring physical activity in the different studies are discussed. Finally, novel perspectives of research are proposed to address the flaws in the reviewed studies.
Blue Light Acclimation Reduces the Photoinhibition of Phalaenopsis aphrodite (Moth Orchid)
Authors: Ko Swee-Suak , Jhong Chung-Min , Shih Ming-Che
Published: August 2020

The moth orchid is an important ornamental crop. It is very sensitive to high light irradiation due to photoinhibition. In this study, young orchid tissue culture seedlings and 2.5” potted plants pretreated under blue light (BL, λmax = 450 nm) at 100 µmol m−2 s−1 for 12 days (BL acclimation) were found to have an increased tolerance to high light irradiation. After BL acclimation, orchids had an increased anthocyanin accumulation, enhanced chloroplast avoidance, and increased chlorophyll fluorescence capacity whenever they were exposed to high light of 1000 μmol m−2 s−1 for two weeks (HL). They had higher Fv/Fm, electron transport rate (ETR), chlorophyll content, catalase activity and sucrose content when compared to the control without BL acclimation. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) showed that transcript levels of phototropins, D1, RbcS, PEPCK, Catalase and SUT2 were upregulated in the BL-acclimated orchids. Consequently, BL acclimation orchids had better growth when compared to the control under long-term high light stress. In summary, this study provides a solution, i.e., BL acclimation, to reduce moth orchid photoinhibition and enhance growth before transplantation of the young tissue culture seedlings and potted plants into greenhouses, where they usually suffer from a high light fluctuation problem.
N-Terminus Does Not Govern Protein Turnover of Schizosaccharomyces pombe CENP-A
Authors: Tan Hwei Ling , Zeng Yi Bing , Chen Ee Sin
Published: August 2020

Centromere integrity underlies an essential framework for precise chromosome segregation and epigenetic inheritance. Although centromeric DNA sequences vary among different organisms, all eukaryotic centromeres comprise a centromere-specific histone H3 variant, centromeric protein A (CENP-A), on which other centromeric proteins assemble into the kinetochore complex. This complex connects chromosomes to mitotic spindle microtubules to ensure accurate partitioning of the genome into daughter cells. Overexpression of CENP-A is associated with many cancers and is correlated with its mistargeting, forming extra-centromeric kinetochore structures. The mislocalization of CENP-A can be counteracted by proteolysis. The amino (N)-terminal domain (NTD) of CENP-A has been implicated in this regulation and shown to be dependent on the proline residues within this domain in Saccharomyces cerevisiae CENP-A, Cse4. We recently identified a proline-rich GRANT motif in the NTD of Schizosaccharomyces pombe CENP-A (SpCENP-A) that regulates the centromeric targeting of CENP-A via binding to the CENP-A chaperone Sim3. Here, we investigated whether the NTD is required to confer SpCENP-A turnover (i.e., counter stability) using various truncation mutants of SpCENP-A. We show that sequential truncation of the NTD did not improve the stability of the protein, indicating that the NTD of SpCENP-A does not drive turnover of the protein. Instead, we reproduced previous observations that heterochromatin integrity is important for SpCENP-A stability, and showed that this occurs in an NTD-independent manner. Cells bearing the null mutant of the histone H3 lysine 9 methyltransferase Clr4 (Δclr4), which have compromised constitutive heterochromatin integrity, showed reductions in the proportion of SpCENP-A in the chromatin-containing insoluble fraction of the cell extract, suggesting that heterochromatin may promote SpCENP-A chromatin incorporation. Thus, a disruption in heterochromatin may result in the delocalization of SpCENP-A from chromatin, thus exposing it to protein turnover. Taken together, we show that the NTD is not required to confer SpCENP-A protein turnover.
Early Molecular Events during Onset of Diapause in Silkworm Eggs Revealed by Transcriptome Analysis
Authors: Gong Jing , Zheng Xi , Zhao Shan , Yang Lingzhen , Xue Zhao , Fan Zhengjie , Tang Miao
Published: August 2020

Diapause is a form of dormancy, and Bombyx mori silkworm embryos are ideal models for studying diapause in insects. However, molecular events in eggs during the onset of diapause remain unclear. In this study, transcriptome analyses were performed on silkworm diapause eggs via RNA sequencing at 20 and 48 h after oviposition. A total of 6402 differentially expressed genes (DEGs) were detected in diapause eggs at 48 h versus that at 20 h after oviposition. Gene ontology enrichment analysis showed that DEGs in diapause eggs at 48 h versus that at 20 h after oviposition were involved in ribosome-related metabolism and hydrogen transport. Kyoto Encyclopedia of Genes and Genomes analysis revealed several significantly enriched biological pathways, namely the oxidative phosphorylation, Forkhead box protein O3 (FoxO) signaling, ribosome, endoplasmic reticular protein processing, and autophagy pathways. Fifteen DEGs from the FoxO signaling pathway were selected, and their expression profiles were consistent with the transcriptome results from real-time quantitative reverse transcription polymerase chain reaction. Our results can improve understanding of the diapause mechanism in silkworm eggs and identified key pathways for future studies.
Mitoquinone (MitoQ) Inhibits Platelet Activation Steps by Reducing ROS Levels
Authors: Méndez Diego , Arauna Diego , Fuentes Francisco , Araya-Maturana Ramiro , Palomo Iván , Alarcón Marcelo , Sebastián David , Zorzano Antonio , Fuentes Eduardo
Published: August 2020

Platelet activation plays a key role in cardiovascular diseases. The generation of mitochondrial reactive oxygen species (ROS) has been described as a critical step required for platelet activation. For this reason, it is necessary to find new molecules with antiplatelet activity and identify their mechanisms of action. Mitoquinone (MitoQ) is a mitochondria-targeted antioxidant that reduces mitochondrial overproduction of ROS. In this work, the antiplatelet effect of MitoQ through platelet adhesion and spreading, secretion, and aggregation was evaluated. Thus MitoQ, in a non-toxic effect, decreased platelet adhesion and spreading on collagen surface, and expression of P-selectin and CD63, and inhibited platelet aggregation induced by collagen, convulxin, thrombin receptor activator peptide-6 (TRAP-6), and phorbol 12-myristate 13-acetate (PMA). As an antiplatelet mechanism, we showed that MitoQ produced mitochondrial depolarization and decreased ATP secretion. Additionally, in platelets stimulated with antimycin A and collagen MitoQ significantly decreased ROS production. Our findings showed, for the first time, an antiplatelet effect of MitoQ that is probably associated with its mitochondrial antioxidant effect.
The Role of Amino Acids in Neurotransmission and Fluorescent Tools for Their Detection
Authors: Dalangin Rochelin , Kim Anna , Campbell Robert E.
Published: August 2020

Neurotransmission between neurons, which can occur over the span of a few milliseconds, relies on the controlled release of small molecule neurotransmitters, many of which are amino acids. Fluorescence imaging provides the necessary speed to follow these events and has emerged as a powerful technique for investigating neurotransmission. In this review, we highlight some of the roles of the 20 canonical amino acids, GABA and β-alanine in neurotransmission. We also discuss available fluorescence-based probes for amino acids that have been shown to be compatible for live cell imaging, namely those based on synthetic dyes, nanostructures (quantum dots and nanotubes), and genetically encoded components. We aim to provide tool developers with information that may guide future engineering efforts and tool users with information regarding existing indicators to facilitate studies of amino acid dynamics.
MicroRNA miR-181—A Rheostat for TCR Signaling in Thymic Selection and Peripheral T-Cell Function
Authors: Grewers Zoe , Krueger Andreas
Published: August 2020

The selection of T cells during intra-thymic d evelopment is crucial to obtain a functional and simultaneously not self-reactive peripheral T cell repertoire. However, selection is a complex process dependent on T cell receptor (TCR) thresholds that remain incompletely understood. In peripheral T cells, activation, clonal expansion, and contraction of the active T cell pool, as well as other processes depend on TCR signal strength. Members of the microRNA (miRNA) miR-181 family have been shown to be dynamically regulated during T cell development as well as dependent on the activation stage of T cells. Indeed, it has been shown that expression of miR-181a leads to the downregulation of multiple phosphatases, implicating miR-181a as ‘‘rheostat’’ of TCR signaling. Consistently, genetic models have revealed an essential role of miR-181a/b-1 for the generation of unconventional T cells as well as a function in tuning TCR sensitivity in peripheral T cells during aging. Here, we review these broad roles of miR-181 family members in T cell function via modulating TCR signal strength.
The Role of NLRP3 Inflammasome in the Pathogenesis of Traumatic Brain Injury
Authors: Irrera Natasha , Russo Massimo , Pallio Giovanni , Bitto Alessandra , Mannino Federica , Minutoli Letteria , Altavilla Domenica , Squadrito Francesco
Published: August 2020

Traumatic brain injury (TBI) represents an important problem of global health. The damage related to TBI is first due to the direct injury and then to a secondary phase in which neuroinflammation plays a key role. NLRP3 inflammasome is a component of the innate immune response and different diseases, such as neurodegenerative diseases, are characterized by NLRP3 activation. This review aims to describe NLRP3 inflammasome and the consequences related to its activation following TBI. NLRP3, caspase-1, IL-1β, and IL-18 are significantly upregulated after TBI, therefore, the use of nonspecific, but mostly specific NLRP3 inhibitors is useful to ameliorate the damage post-TBI characterized by neuroinflammation. Moreover, NLRP3 and the molecules associated with its activation may be considered as biomarkers and predictive factors for other neurodegenerative diseases consequent to TBI. Complications such as continuous stimuli or viral infections, such as the SARS-CoV-2 infection, may worsen the prognosis of TBI, altering the immune response and increasing the neuroinflammatory processes related to NLRP3, whose activation occurs both in TBI and in SARS-CoV-2 infection. This review points out the role of NLRP3 in TBI and highlights the hypothesis that NLRP3 may be considered as a potential therapeutic target for the management of neuroinflammation in TBI.
Impact of Bi and Sn on Microstructure and Corrosion Resistance of Zinc Coatings Obtained in Zn-AlNi Bath
Authors: Kania Henryk , Saternus Mariola , Kudláček Jan
Published: August 2020

The paper presents results of studies on the impact of bismuth and tin additions to the Zn-AlNi bath on microstructure and corrosion resistance of hot dip galvanizig coatings. The structure at high magnifications on the top surface and cross-section of coatings received in the Zn-AlNiBiSn bath was revealed and the microanalysis EDS (energy dispersion spectroscopy) of chemical composition was determined. The corrosion resistance of the coatings was tested relatively in a neutral salt spray test (NSS), and tests in a humid atmosphere containing SO2. Electrochemical parameters of coatings corrosion were determined. It was found that Zn-AlNiBiSn coatings show lower corrosion resistance in comparison with the coatings received in the Zn-AlNi bath without Sn and Bi alloying additions. Structural research has shown the existence of precipitations of Sn-Bi alloy in the coating. It was found that Sn-Bi precipitations have more electropositive potential in relation to zinc, which promotes the formation of additional corrosion cells.
Asymmetric Synthesis of Tertiary α -Hydroxyketones by Enantioselective Decarboxylative Chlorination and Subsequent Nucleophilic Substitution
Authors: Kam Mei Kee , Sugiyama Akira , Kawanishi Ryouta , Shibatomi Kazutaka
Published: August 2020

Chiral tertiary α-hydroxyketones were synthesized with high enantiopurity by asymmetric decarboxylative chlorination and subsequent nucleophilic substitution. We recently reported the asymmetric decarboxylative chlorination of β-ketocarboxylic acids in the presence of a chiral primary amine catalyst to obtain α-chloroketones with high enantiopurity. Here, we found that nucleophilic substitution of the resulting α-chloroketones with tetrabutylammonium hydroxide yielded the corresponding α-hydroxyketones without loss of enantiopurity. The reaction proceeded smoothly even at a tertiary carbon. The proposed method would be useful for the preparation of chiral tertiary alcohols.
Pharmacological Aspects of Over-the-Counter Opioid Drugs Misuse
Authors: Sobczak Łukasz , Goryński Krzysztof
Published: August 2020

Several over-the-counter (OTC) drugs are known to be misused. Among them are opioids such as codeine, dihydrocodeine, and loperamide. This work elucidates their pharmacology, interactions, safety profiles, and how pharmacology is being manipulated to misuse these common medications, with the aim to expand on the subject outlined by the authors focusing on abuse prevention and prevalence rates. The reviewed literature was identified in several online databases through searches conducted with phrases created by combining the international non-proprietary names of the drugs with terms related to drug misuse. The results show that OTC opioids are misused as an alternative for illicit narcotics, or prescription-only opioids. The potency of codeine and loperamide is strongly dependent on the individual enzymatic activity of CYP2D6 and CYP3A4, as well as P-glycoprotein function. Codeine can also be utilized as a substrate for clandestine syntheses of more potent drugs of abuse, namely desomorphine (“Krokodil”), and morphine. The dangerous methods used to prepare these substances can result in poisoning from toxic chemicals and impurities originating from the synthesis procedure. OTC opioids are generally safe when consumed in accordance with medical guidelines. However, the intake of supratherapeutic amounts of these substances may reveal surprising traits of common medications.
Influence of the Molecular Weight and the Presence of Calcium Ions on the Molecular Interaction of Hyaluronan and DPPC
Authors: Zander Thomas , Garamus Vasil M. , Dédinaité Andra , Claesson Per M. , Bełdowski Piotr , Górny Krzysztof , Dendzik Zbigniew , Wieland D. C. Florian , Willumeit-Römer Regine
Published: August 2020

Hyaluronan is an essential physiological bio macromolecule with different functions. One prominent area is the synovial fluid which exhibits remarkable lubrication properties. However, the synovial fluid is a multi-component system where different macromolecules interact in a synergetic fashion. Within this study we focus on the interaction of hyaluronan and phospholipids, which are thought to play a key role for lubrication. We investigate how the interactions and the association structures formed by hyaluronan (HA) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) are influenced by the molecular weight of the bio polymer and the ionic composition of the solution. We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics). The interaction of hyaluronan and phospholipids depends on the molecular weight, where hyaluronan with lower molecular weight has the strongest interaction. Furthermore, the interaction is increased by the presence of calcium ions. Our simulations show that calcium ions are located close to the carboxylate groups of HA and, by this, reduce the number of formed hydrogen bonds between HA and DPPC. The observed change in the DPPC phase behavior can be attributed to a local charge inversion by calcium ions binding to the carboxylate groups as the binding distribution of hyaluronan and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine is not changed.
Hydrothermal Conversion of Spent Sugar Beets into High-Value Platform Molecules
Authors: Pfersich Jens , Arauzo Pablo J. , Lucian Michela , Modugno Pierpaolo , Titirici Maria-Magdalena , Fiori Luca , Kruse Andrea
Published: August 2020

The growing importance of bio-based products, combined with the desire to decrease the production of wastes, boosts the necessity to use wastes as raw materials for bio-based products. A waste material with a large potential is spent sugar beets, which are mainly used as animal feeds or fertilizers. After hydrothermal treatment, the produced chars exhibited an H/C ratio of 1.2 and a higher heating value of 22.7 MJ/kg, which were similar to that of subbituminous coal and higher than that of lignite. Moreover, the treatment of 25 g/L of glucose and 22 g/L of fructose by heating up to 160 °C led to a possible application of spent sugar beets for the production of 5-hydroxymethylfurfural. In the present study, the maximum concentration of 5-hydroxymethylfurfural was 3.4 g/L after heating up to 200 °C.
Nucleotides and AHCC Enhance Th1 Responses In Vitro in Leishmania-Stimulated/Infected Murine Cells
Authors: Dea-Ayuela María Auxiliadora , Segarra Sergi , Serrano Dolores R. , Bolás-Fernández Francisco
Published: August 2020

A stronger Th1 (cellular) immune response in canine leishmaniosis (CanL) leads to a better prognosis. Dietary nucleotides plus AHCC® have shown beneficial effects in dogs with clinical leishmaniosis and in clinically healthy Leishmania-infected dogs. The potential leishmanicidal activity of nucleotides and AHCC was assessed by quantifying nitric oxide (NO) production and replication of parasites. Their effects on lymphocyte proliferation were studied with and without soluble Leishmania infantum antigen (SLA) stimulation. Cytokine level variations were assessed using naïve and L. infantum-infected macrophages/lymphocytes cocultures. Promastigotes and amastigotes proliferation and NO macrophage production were not directly affected. Lymphocyte proliferation was significantly enhanced by nucleotides, AHCC, and their combinations only after SLA stimulation. Nucleotides and AHCC significantly increased the production of IL-1β, IL-2, IL-5, IL-9, IL-10, and IL-12 by naïve immune cells. In naïve and L. infantum-infected macrophage/lymphocyte cocultures, nucleotides with or without AHCC led to significant increases in IFN-γ and TNF-α. Given that these cytokines are involved in the effective Th1 immune response against Leishmania parasites, these mechanisms of action could explain the previously reported in vivo clinical efficacy of such combination and further support the use of nucleotides with or without AHCC in the management of CanL patients.
Age-Related Changes and Sex-Related Differences in Brain Iron Metabolism
Authors: Grubić Kezele Tanja , Ćurko-Cofek Božena
Published: August 2020

Iron is an essential element that participates in numerous cellular processes. Any disruption of iron homeostasis leads to either iron deficiency or iron overload, which can be detrimental for humans’ health, especially in elderly. Each of these changes contributes to the faster development of many neurological disorders or stimulates progression of already present diseases. Age-related cellular and molecular alterations in iron metabolism can also lead to iron dyshomeostasis and deposition. Iron deposits can contribute to the development of inflammation, abnormal protein aggregation, and degeneration in the central nervous system (CNS), leading to the progressive decline in cognitive processes, contributing to pathophysiology of stroke and dysfunctions of body metabolism. Besides, since iron plays an important role in both neuroprotection and neurodegeneration, dietary iron homeostasis should be considered with caution. Recently, there has been increased interest in sex-related differences in iron metabolism and iron homeostasis. These differences have not yet been fully elucidated. In this review we will discuss the latest discoveries in iron metabolism, age-related changes, along with the sex differences in iron content in serum and brain, within the healthy aging population and in neurological disorders such as multiple sclerosis, Parkinson’s disease, Alzheimer’s disease, and stroke.
Who Reads Food Labels? Selected Predictors of Consumer Interest in Front-of-Package and Back-of-Package Labels during and after the Purchase
Authors: Bryła Paweł
Published: August 2020

The paper aims to identify selected predictors of food label use to extend our knowledge about consumer behavior related to food purchases. Two types of information were examined: front-of-package (FOP) and back-of-package (BOP), and two contexts of reading labels were distinguished: during shopping and at home. Various types of potential predictors were tested, including demographic (e.g., age, gender, household size, place of living), socioeconomic (e.g., education, professional activity, income), behavioral (e.g., purchasing certain types of products), and psychographic (e.g., importance attached to various types of information) criteria. The survey was conducted with the use of the CAWI (Computer-Assisted Web Interviews) methodology in a sample of 1051 Polish consumers. Quota sampling was applied based on sex, age, education, place of living (urban vs. rural), and region. Descriptive statistics, t-tests, ANOVAs, Pearson correlation coefficients, and multiple and retrograde step regressions were applied. In retrograde step regression models, only one predictor (self-rated knowledge about nutrition healthiness) turned out to be significant for all four measures of label reading. The remaining predictors were specific to selected measures of reading labels. The importance of the information about the content of fat and that about the health effects of consuming a food product were significant predictors of three types of food label use. This study confirms the necessity to investigate reading labels in fine-grained models, adapted to different types of labels and different contexts of reading. Our results show that demographic or socioeconomic variables are not significant predictors of reading food labels for a large group of Polish consumers.
Effectiveness of Protein Supplementation Combined with Resistance Training on Muscle Strength and Physical Performance in Elderly: A Systematic Review and Meta-Analysis
Authors: Labata-Lezaun Noé , Llurda-Almuzara Luis , López-de-Celis Carlos , Rodríguez-Sanz Jacobo , González-Rueda Vanessa , Hidalgo-García César , Muniz-Pardos Borja , Pérez-Bellmunt Albert
Published: August 2020

The aim of this study was to evaluate the effectiveness of the combination of resistance training (RT) and protein supplementation (PS), compared to RT alone or combined with a placebo (plS), in the improvement of muscle strength and physical performance. The search strategy in PubMed, Cochrane Library, and Web of Sciences databases found a total of 294 studies. Once inclusion and exclusion criteria were applied, 16 studies were included for the qualitative analysis. A total of 657 healthy elderly (>60 years) participants were analysed. Finally, 15 articles were included in the quantitative analysis with one being excluded due to issues with data availability. Upper-limb, lower-limb, and handgrip strength were the primary outcomes of the meta-analysis. The secondary outcomes, related to physical performance, were Short Physical Performance Battery (SPPB), gait speed, and the five-chair-rise test (5CRT). The main results of the meta-analysis show no statistical differences for upper-limb (SMD: 0.56, 95% CI: −0.09, 1.21, p = 0.09, I2 = 68%), lower-limb (SMD: 0.00, 95% CI: −0.18, 0.18, p = 1.0, I2 = 11%), and handgrip strength (SMD: 0.03, 95% CI: −0.26, 0.32, p = 0.84, I2 = 0%) between the RT + PS and the RT alone (or combined with plS). Moreover, no statistical differences were found relating to physical performance. In view of these results, protein supplementation combined with RT does not provide additional benefits compared to RT alone or with plS in healthy elderly adults.
24-h Urine Collection: A Relevant Tool in CKD Nutrition Evaluation
Authors: Abdel-Nabey Moustafa , Saint-Jacques Camille , Boffa Jean-Jacques , Frochot Vincent , Livrozet Marine , Daudon Michel , Flamant Martin , Letavernier Emmanuel , Haymann Jean-Philippe
Published: August 2020

Dietary management is a cornerstone of Chronic Kidney Disease (CKD) monitoring, and dietary surveys often difficult to perform. We studied in a CKD patient cohort with two years follow-up, whether validated 24-h urine ionogram would be a relevant tool for diet evaluation and compliance. We included 404 non-dialysis CKD patients, with three evaluations, including repeated measurements of fractional renal creatinine clearance and 24-h urine collection. Completeness of the 24-h urine collection, assessed by daily urine creatinine excretion extrapolated from fractional creatinine clearance, was 64.6%, 75.5%, and 78.2% at the first, second, and third visits, respectively. One hundred sixty-eight patients (41.6%) had three complete collections, with a measured glomerular filtration of 42.3 mL/min/1.73 m2 at baseline and prevalence of anemia and secondary hyperparathyroidism of 13.9% and 26.2%, respectively, increasing during follow-up to 15% and 31.5% (p < 0.001 and p < 0.001). The urine analysis showed at baseline a urine volume of above 2 L/day, and estimated sodium and protein intake within targets in 51.6% and 40.3% of cases, which improved during follow-up only for protein (to 45.9%, p < 0.0001). Our data suggest that a 24-h urine ionogram is an interesting, reliable tool in CKD patients for dietary monitoring to achieve target recommendation noteworthy salt and protein intake.
Curcumin Diglutaric Acid, a Prodrug of Curcumin Reduces Pain Hypersensitivity in Chronic Constriction Injury of Sciatic Nerve Induced-Neuropathy in Mice
Authors: Limcharoen Thanchanok , Dasuni Wasana Peththa Wadu , Hasriadi Hasriadi , Muangnoi Chawanphat , Vajragupta Opa , Rojsitthisak Pornchai , Towiwat Pasarapa
Published: August 2020

The drug treatment for neuropathic pain remains a challenge due to poor efficacy and patient satisfaction. Curcumin has been reported to alleviate neuropathic pain, but its clinical application is hindered by its low solubility and poor oral bioavailability. Curcumin diglutaric acid (CurDG) is a curcumin prodrug with improved water solubility and in vivo antinociceptive effects. In this study, we investigated the anti-inflammatory mechanisms underlying the analgesic effect of CurDG in the chronic constriction injury (CCI)-induced neuropathy mouse model. Repeated oral administration of CurDG at a low dose equivalent to 25 mg/kg/day produced a significant analgesic effect in this model, both anti-allodynic activity and anti-hyperalgesic activity appearing at day 3 and persisting until day 14 post-CCI surgery (p < 0.001) while having no significant effect on the motor performance. Moreover, the repeated administration of CurDG diminished the increased levels of the pro-inflammatory cytokines: TNF-α and IL-6 in the sciatic nerve and the spinal cord at the lowest tested dose (equimolar to 25 mg/kg curcumin). This study provided pre-clinical evidence to substantiate the potential of pursuing the development of CurDG as an analgesic agent for the treatment of neuropathic pain.
Improving Tumor Retention of Effector Cells in Adoptive Cell Transfer Therapies by Magnetic Targeting
Authors: Sanz-Ortega Laura , Rojas José Manuel , Barber Domingo F.
Published: August 2020

Adoptive cell transfer therapy is a promising anti-tumor immunotherapy in which effector immune cells are transferred to patients to treat tumors. However, one of its main limitations is the inefficient trafficking of inoculated effector cells to the tumor site and the small percentage of effector cells that remain activated when reaching the tumor. Multiple strategies have been attempted to improve the entry of effector cells into the tumor environment, often based on tumor types. It would be, however, interesting to develop a more general approach, to improve and facilitate the migration of specific activated effector lymphoid cells to any tumor type. We and others have recently demonstrated the potential for adoptive cell transfer therapy of the combined use of magnetic nanoparticle-loaded lymphoid effector cells together with the application of an external magnetic field to promote the accumulation and retention of lymphoid cells in specific body locations. The aim of this review is to summarize and highlight the recent findings in the field of magnetic accumulation and retention of effector cells in tumors after adoptive transfer, and to discuss the possibility of using this approach for tumor targeting with chimeric antigen receptor (CAR) T-cells.
Thermal, Viscoelastic, Mechanical and Wear Behaviour of Nanoparticle Filled Polytetrafluoroethylene: A Comparison
Authors: Tóth Levente Ferenc , De Baets Patrick , Szebényi Gábor
Published: August 2020

In this research work, unfilled and mono-filled polytetrafluoroethylene (PTFE) materials were developed and characterised by physical, thermal, viscoelastic, mechanical, and wear analysis. The applied fillers were graphene, alumina (Al2O3), boehmite alumina (BA80), and hydrotalcite (MG70) in 0.25/1/4/8 and 16 wt % filler content. All samples were produced by room temperature pressing–free sintering method. All of the fillers were blended with PTFE by intensive dry mechanical stirring; the efficiency of the blending was analysed by Energy-dispersive X-ray spectroscopy (EDS) method. Compared to neat PTFE, graphene in 4/8/16 wt % improved the thermal conductivity by ~29%/~84%/~157%, respectively. All fillers increased the storage, shear and tensile modulus and decreased the ductility. PTFE with 4 wt % Al2O3 content reached the lowest wear rate; the reduction was more than two orders of magnitude compared to the neat PTFE.
Analyzing Spatiotemporal Variation Modes and Industry-Driving Force Research Using VIIRS Nighttime Light in China
Authors: Song Xiaoke , Chen Yunhao , Li Kangning
Published: August 2020

Urbanization is a complex process closely involving the economy, society, and population. While monitoring urban development and exploring the industry-driving force in a real-time and effective way are the prerequisites for optimizing industry structure, narrowing the urban development gap, and achieving sustainable development. Nighttime light is an effective tool to monitor urban development from a macro perspective. However, the systematic research of nighttime light spatiotemporal variation modes and the industry-driving force of urban nighttime light are still unknown. Considering these issues, this paper analyzes the spatiotemporal variation modes of the average light index (ALI) and investigates the industry-driving force of ALI in 100 major prefecture-level cities across China mainland based on National Polar-Orbiting Partnership Satellite Visible Infrared Imaging Radiometer Suite (NPP VIIRS). The conclusions are as following three aspects. First, ALI is observed a funnel pattern among four regions in spatial dimension, with low in center and high in the surrounding, and it shows 5 variation modes (“W,” “√,” “Exponent,” “Logarithm,” and “N”) in temporal dimension, of which the “√” mode accounts for the highest proportion (60%). Second, the industry structure is closely related to ALI. Besides, the factor analysis result illustrates that the secondary and tertiary industry are the driving industries of ALI. Third, the classification result based on the industry contribution rate indicates that cities driven by different industries show significant spatial distribution differences. The three major industry-driving cities are mainly distributed in central and western regions, the secondary and tertiary industry-driving cities are evenly distributed, and the tertiary industry-driving cities are mainly distributed in provincial capitals. From 2013 to 2018, the fluctuation of city distribution driven by different industries changes obviously. The number of tertiary industry-driving cities increases steadily and the three major industry-driving cities are distributed wider spatially. Additionally, the impacts of location and raw coal on ALI are discussed. In general, these findings are essential to further research urban development mode and can be considered as the reference to narrow urban development gap.