bluuebunny/crossref_metadata_2025 huggingface dataset:
Betulin Stimulates Osteogenic Differentiation of Human Osteoblasts-Loaded Alginate–Gelatin Microbeads
Authors: Karaca Mehmet Ali , Kancagi Derya Dilek , Ozbek Ugur , Ovali Ercument , Gok Ozgul
Published: May 2024

Osteoporosis, a terminal illness, has emerged as a global public health problem in recent years. The long-term use of bone anabolic drugs to treat osteoporosis causes multi-morbidity in elderly patients. Alternative therapies, such as allogenic and autogenic tissue grafts, face important issues, such as a limited source of allogenic grafts and tissue rejection in autogenic grafts. However, stem cell therapy has been shown to increase bone regeneration and decrease osteoporotic bone formation. Stem cell therapy combined with betulin (BET) supplementation might be adequate for bone remodeling and new bone tissue generation. In this study, the effect of BET on the viability and osteogenic differentiation of hFOB 1.19 cells was investigated. The cells were encapsulated in alginate–gelatin (AlGel) microbeads. In vitro tests were conducted during the 12 d of incubation. While BET showed cytotoxic activity (>1 µM) toward non-encapsulated hFOB 1.19 cells, encapsulated cells retained their functionality for up to 12 days, even at 5 µM BET. Moreover, the expression of osteogenic markers indicates an enhanced osteo-inductive effect of betulin on encapsulated hFOB 1.19, compared to the non-encapsulated cell culture. The 3D micro-environment of the AlGel microcapsules successfully protects the hFOB 1.19 cells against BET cytotoxicity, allowing BET to improve the mineralization and differentiation of osteoblast cells.
Evaluating Sustainable Practices for Managing Residue Derived from Wheat Straw
Authors: Shanmugam Harikishore , Raghavan Vijaya , Rajagopal Rajinikanth , Goyette Bernard , Lyu Linxiang , Zhou Siyuan , An Chunjiang
Published: May 2024

Farm leftovers, particularly crop residues, are a key source of renewable energy in Canada. The nation’s robust agricultural industry provides ample biomass, derived from forestry and agriculture resources, for energy generation. Crop residues, such as straws and husks, play a crucial role in this biomass reservoir, contributing to biofuel production and greenhouse gas mitigation efforts. Focusing on supply chains, waste management, and emission reduction, this study evaluates the sustainability of wheat straw, an agricultural biomass by-product. The environmental issues of various approaches to managing agricultural biomass were explored. Following an evaluation of biomass features, conversion methods, and economic and environmental advantages, the results show anaerobic digestion to be the most sustainable approach. Four metrics were examined in relation to social elements, and numerous aspects were considered as inputs in the evaluation of transportation costs. The use of electric trucks versus fuel-based trucks resulted in an 18% reduction in total operating costs and a 58% reduction in consumption costs. This study examined CO2 emissions over four different transportation distances. The data indicate that a significant reduction of 36% in kg CO2 equivalent emissions occurred when the distance was lowered from 100 km to 25 km. These findings offer insights for creating practical plans that should increase the sustainability of agricultural biomass leftovers.
M2 Macrophages Guide Periosteal Stromal Cell Recruitment and Initiate Bone Injury Regeneration
Authors: Lu Dazhuang , Zhang Yingfei , Liang Shimin , Li Yang , Qing Jia , Gu Lanxin , Xu Xiuyun , Wang Zeying , Gao Xin , Liu Hao , Zhang Xiao , Zhou Yongsheng , Zhang Ping
Published: May 2024

The periosteum plays a critical role in bone repair and is significantly influenced by the surrounding immune microenvironment. In this study, we employed 10× single-cell RNA sequencing to create a detailed cellular atlas of the swine cranial periosteum, highlighting the cellular dynamics and interactions essential for cranial bone injury repair. We noted that such injuries lead to an increase in M2 macrophages, which are key in modulating the periosteum’s immune response and driving the bone regeneration process. These macrophages actively recruit periosteal stromal cells (PSCs) by secreting Neuregulin 1 (NRG1), a crucial factor in initiating bone regeneration. This recruitment process emphasizes the critical role of PSCs in effective bone repair, positioning them as primary targets for therapeutic interventions. Our results indicate that enhancing the interaction between M2 macrophages and PSCs could significantly improve the outcomes of treatments aimed at cranial bone repair and regeneration.
Atypical Endometriosis: A Comprehensive Systematic Review of Pathological Patterns and Diagnostic Challenges
Authors: Capozzi Vito Andrea , Scarpelli Elisa , dell’Omo Sara , Rolla Martino , Pezzani Alessandra , Morganelli Giovanni , Gaiano Michela , Ghi Tullio , Berretta Roberto
Published: May 2024

Endometriosis is a benign condition affecting women of reproductive age. A potential association with ovarian cancer has been documented. Atypical endometriosis (AE) is characterized by deviations from the typical microscopic appearance of endometriosis, including cytologic and architectural atypia. AE has been recognized as a potential precursor to endometriosis-associated ovarian cancers (EAOC), particularly endometrioid and clear cell subtypes. AE presents challenges in diagnosis due to its diverse clinical and pathological features, often requiring careful histological evaluation for accurate identification. Architectural AE, defined by localized proliferation of crowded glands with atypical epithelium resembling endometrial neoplasia, and cytologic AE, characterized by nuclear atypia within the epithelial lining of endometriotic cysts, are key subtypes. Immunohistochemical and molecular studies have revealed aberrant expression of markers such as Ki67, COX-2, BAF250a, p53, estrogen receptor, progesterone receptor, and IMP-3. Long-term follow-up studies suggest relatively low recurrence and malignant transformation rates among patients with AE, but uncertainties persist regarding its exact malignancy potential and optimal management strategies. Integration of artificial intelligence and shared molecular aberrations between AE and EAOC may enhance diagnostic accuracy. Continuous interdisciplinary collaboration and ongoing research efforts are crucial for a deeper understanding of the relationship between endometriosis and carcinogenesis, ultimately improving patient care and surveillance.
Temporal Association Rule Mining: Race-Based Patterns of Treatment-Adverse Events in Breast Cancer Patients Using SEER–Medicare Dataset
Authors: Adam Nabil , Wieder Robert
Published: May 2024

PURPOSE: Disparities in the screening, treatment, and survival of African American (AA) patients with breast cancer extend to adverse events experienced with systemic therapy. However, data are limited and difficult to obtain. We addressed this challenge by applying temporal association rule (TAR) mining using the SEER–Medicare dataset for differences in the association of specific adverse events (AEs) and treatments (TRs) for breast cancer between AA and White women. We considered two categories of cancer care providers and settings: practitioners providing care in the outpatient units of hospitals and institutions and private practitioners providing care in their offices. PATIENTS AN METHODS: We considered women enrolled in the Medicare fee-for-service option at age 65 who qualified by age and not disability, who were diagnosed with breast cancer with attributed patient factors of age and race, marital status, comorbidities, prior malignancies, prior therapy, disease factors of stage, grade, and ER/PR and Her2 status and laterality. We included 141 HCPCS drug J codes for chemotherapy, biotherapy, and hormone therapy drugs, which we consolidated into 46 mechanistic categories and generated AE data. We consolidated AEs from ICD9 codes into 18 categories associated with breast cancer therapy. We applied TAR mining to determine associations between the 46 TR and 18 AE categories in the context of the patient categories outlined. We applied the spark.mllib implementation of the FPGrowth algorithm, a parallel version called PFP. We considered differences of at least one unit of lift as significant between groups. The model’s results demonstrated a high overlap between the model’s identified TR-AEs associated set and the actual set. RESULTS: Our results demonstrate that specific TR/AE associations are highly dependent on race, stage, and venue of care administration. CONCLUSIONS: Our data demonstrate the usefulness of this approach in identifying differences in the associations between TRs and AEs in different populations and serve as a reference for predicting the likelihood of AEs in different patient populations treated for breast cancer. Our novel approach using unsupervised learning enables the discovery of association rules while paying special attention to temporal information, resulting in greater predictive and descriptive power as a patient’s health and life status change over time.
Mild Disease Course of Experimental Autoimmune Encephalomyelitis without Pertussis Toxin: Brain Transcriptome Analysis Reveals Similar Signaling to Active Lesions in Multiple Sclerosis
Authors: Frodella Christa M. , Pruett Stephen B. , Kaplan Barbara L. F.
Published: May 2024

Experimental autoimmune encephalomyelitis (EAE) is a powerful model to study multiple sclerosis (MS). One of the approaches for EAE is to actively immunize with myelin-derived peptides with immune adjuvants. One of the commonly used immune adjuvants is pertussis toxin (PTx), without which EAE disease is mild with relatively longer onset. However, pertussis toxin can also inhibit G protein-coupled receptor (GPCR) signaling so it can confound investigations into the role of GPCRs in EAE or therapies designed to target GPCRs. Since EAE via active immunization without PTx results in a relatively mild disease state, we wanted to confirm that appropriate signaling molecules for the disease were being induced in one target tissue (i.e., brain). RNA-Seq analysis of whole brain tissue demonstrated that the MS signaling pathway was strongly activated in symptomatic mice. In addition, there was activation of Th1 (IFN signaling), Th2 (IL-4 signaling), and Th17 (IL-17 signaling). In comparing canonical pathways from our mouse mild EAE brains with a human MS atlas, EAE shared the most pathways with active and inactive lesions. An advantage of this approach is that disease induction is slower to develop and results in modest clinical signs, which likely more closely mimic human disease onset.
Cardiovascular Autonomic Dysfunction in Hospitalized Patients with a Bacterial Infection: A Longitudinal Observational Pilot Study in the UK
Authors: Arias-Colinas Monica , Gea Alfredo , Kwan Joseph , Vassallo Michael , Allen Stephen C. , Khattab Ahmed
Published: May 2024

Purpose: A temporal reduction in the cardiovascular autonomic responses predisposes patients to cardiovascular instability after a viral infection and therefore increases the risk of associated complications. These findings have not been replicated in a bacterial infection. This pilot study will explore the prevalence of cardiovascular autonomic dysfunction (CAD) in hospitalized patients with a bacterial infection. Methods: A longitudinal observational pilot study was conducted. Fifty participants were included: 13 and 37 participants in the infection group and healthy group, respectively. Recruitment and data collection were carried out during a two-year period. Participants were followed up for 6 weeks: all participants’ cardiovascular function was assessed at baseline (week 1) and reassessed subsequently at week 6 so that the progression of the autonomic function could be evaluated over that period of time. The collected data were thereafter analyzed using STATA/SE version 16.1 (StataCorp). The Fisher Exact test, McNemar exact test, Mann–Whitney test and Wilcoxon test were used for data analysis. Results: 32.4% of the participants in the healthy group were males (n = 12) and 67.6% were females (n = 25). Participants’ age ranged from 33 years old to 76 years old with the majority being 40–60 years of age (62.1%) (Mean age 52.4 SD = 11.4). Heart rate variability (HRV) in response to Valsalva Maneuver, metronome breathing, standing and sustained handgrip in the infection group was lower than in the healthy group throughout the weeks. Moreover, both the HRV in response to metronome breathing and standing up showed a statistically significant difference when the mean values were compared between both groups in week 1 (p = 0.03 and p = 0.013). The prevalence of CAD was significantly higher in the infection group compared to healthy volunteers, both at the beginning of the study (p = 0.018) and at the end of follow up (p = 0.057), when all patients had been discharged. Conclusions: CAD, as assessed by the HRV, is a common finding during the recovery period of a bacterial infection, even after 6 weeks post-hospital admission. This may increase the risk of complications and cardiovascular instability. It may therefore be of value to conduct a wider scale study to further evaluate this aspect so recommendations can be made for the cardiovascular autonomic assessment of patients while they are recovering from a bacterial infectious process.
Autocatalysis, Autopoiesis, and the Opportunity Cost of Individuality
Authors: Kliska Nemanja , Nehaniv Chrystopher L.
Published: May 2024

Ever since Varela and Maturana proposed the concept of autopoiesis as the minimal requirement for life, there has been a focus on cellular systems that erect topological boundaries to separate themselves from their surrounding environment. Here, we reconsider whether the existence of such a spatial boundary is strictly necessary for self-producing entities. This work presents a novel computational model of a minimal autopoietic system inspired by dendrites and molecular dynamic simulations in three-dimensional space. A series of simulation experiments where the metabolic pathways of a particular autocatalytic set are successively inhibited until autocatalytic entities that could be considered autopoietic are produced. These entities maintain their distinctness in an environment containing multiple identical instances of the entities without the existence of a topological boundary. This gives rise to the concept of a metabolic boundary which manifests as emergent self-selection criteria for the processes of self-production without any need for unique identifiers. However, the adoption of such a boundary comes at a cost, as these autopoietic entities are less suited to their simulated environment than their autocatalytic counterparts. Finally, this work showcases a generalized metabolism-centered approach to the study of autopoiesis that can be applied to both physical and abstract systems alike.
Concentration of Microparticles/Cells Based on an Ultra-Fast Centrifuge Virtual Tunnel Driven by a Novel Lamb Wave Resonator Array
Authors: Wei Wei , Wang Zhaoxun , Wang Bingnan , Pang Wei , Yang Qingrui , Duan Xuexin
Published: May 2024

The µTAS/LOC, a highly integrated microsystem, consolidates multiple bioanalytical functions within a single chip, enhancing efficiency and precision in bioanalysis and biomedical operations. Microfluidic centrifugation, a key component of LOC devices, enables rapid capture and enrichment of tiny objects in samples, improving sensitivity and accuracy of detection and diagnosis. However, microfluidic systems face challenges due to viscosity dominance and difficulty in vortex formation. Acoustic-based centrifugation, particularly those using surface acoustic waves (SAWs), have shown promise in applications such as particle concentration, separation, and droplet mixing. However, challenges include accurate droplet placement, energy loss from off-axis positioning, and limited energy transfer from low-frequency SAW resonators, restricting centrifugal speed and sample volume. In this work, we introduce a novel ring array composed of eight Lamb wave resonators (LWRs), forming an Ultra-Fast Centrifuge Tunnel (UFCT) in a microfluidic system. The UFCT eliminates secondary vortices, concentrating energy in the main vortex and maximizing acoustic-to-streaming energy conversion. It enables ultra-fast centrifugation with a larger liquid capacity (50 μL), reduced power usage (50 mW) that is one order of magnitude smaller than existing devices, and greater linear speed (62 mm/s), surpassing the limitations of prior methods. We demonstrate successful high-fold enrichment of 2 μm and 10 μm particles and explore the UFCT’s potential in tissue engineering by encapsulating cells in a hydrogel-based micro-organ with a ring structure, which is of great significance for building more complex manipulation platforms for particles and cells in a bio-compatible and contactless manner.
Sensitive Detection of Oral Leukoplakia: Analyzing P90 Biomarkers in Saliva and Tissue
Authors: Wan Hsiao-Hsuan , Zhu Haochen , Chiang Chao-Ching , Li Jian-Sian , Ren Fan , Tsai Cheng-Tse , Liao Yu-Te , Neal Dan , Katz Joseph , Esquivel-Upshaw Josephine F.
Published: May 2024

Oral cancer represents a significant global public health challenge, contributing substantially to the incidence and mortality of cancer. Despite established risk factors such as tobacco use and alcohol consumption, early detection remains crucial for effective treatment. This study introduces a novel approach using a transistor-based biosensor system for detecting the P90 (CIP2A) protein. We tested the presence of CIP2A in human leukoplakia samples, which can undergo malignant conversion into aggressive oral squamous cell carcinoma. The method used commercially available glucose test strips functionalized with P90 antibodies, providing high sensitivity and a low limit of detection which was five orders lower than that of commercial ELISA kits. A specially designed printed circuit board (PCB) facilitated accurate measurements, and the device’s performance was optimized through characteristic tests. Human sample testing validated the biosensor’s effectiveness in distinguishing samples after cell lysis. This study contributes to advancing accurate and cost-effective diagnostic approaches for oral pre-cancer and cancer tissues.
Neonatal Physical Growth Predicts Electroencephalography Power in Rural South African Children
Authors: Tarullo Amanda R. , Evans Denise , Coetzee Lezanie , Lopera-Perez Diana C. , Brady Shaina P. , Gabard-Durnam Laurel J. , Fink Günther , Hamer Davidson H. , Yousafzai Aisha K. , Rockers Peter C.
Published: May 2024

Anthropometric measures at birth, indexing prenatal growth, are associated with later cognitive development. Children in low- and middle-income countries (LMIC) are at elevated risk for impaired prenatal and early postnatal growth and enduring cognitive deficits. However, the associations of neonatal physical growth with neural activity are not well-characterized in LMIC contexts, given the dearth of early childhood neuroimaging research in these settings. The current study examined birth length, weight, and head circumference as predictors of EEG relative power over the first three years of life in rural Limpopo Province, South Africa, controlling for postnatal growth and socioeconomic status (SES). A larger head circumference at birth predicted lower relative gamma power, lower right hemisphere relative beta power, and higher relative alpha and theta power. A greater birth length also predicted lower relative gamma power. There were interactions with timepoints such that the associations of birth head circumference and length with EEG power were most pronounced at the 7-month assessment and were attenuated at the 17- and 36-month assessments. The results identify birth head circumference and length as specific predictors of infant neural activity within an under-resourced context.
Unraveling the Epigenetic Landscape: Insights into Parkinson’s Disease, Amyotrophic Lateral Sclerosis, and Multiple Sclerosis
Authors: Di Martino Pierpaolo , Marcozzi Valentina , Bibbò Sandra , Ghinassi Barbara , Di Baldassarre Angela , Gaggi Giulia , Di Credico Andrea
Published: May 2024

Parkinson’s disease (PD), multiple sclerosis (MS), and amyotrophic lateral sclerosis (ALS) are examples of neurodegenerative movement disorders (NMDs), which are defined by a gradual loss of motor function that is frequently accompanied by cognitive decline. Although genetic abnormalities have long been acknowledged as significant factors, new research indicates that epigenetic alterations are crucial for the initiation and development of disease. This review delves into the complex interactions that exist between the pathophysiology of NMDs and epigenetic mechanisms such DNA methylation, histone modifications, and non-coding RNAs. Here, we examine how these epigenetic changes could affect protein aggregation, neuroinflammation, and gene expression patterns, thereby influencing the viability and functionality of neurons. Through the clarification of the epigenetic terrain underpinning neurodegenerative movement disorders, this review seeks to enhance comprehension of the underlying mechanisms of the illness and augment the creation of innovative therapeutic strategies.
Protein Kinase C (PKC) in Neurological Health: Implications for Alzheimer’s Disease and Chronic Alcohol Consumption
Authors: Singh Nishtha , Nandy Shouvik Kumar , Jyoti Anupam , Saxena Juhi , Sharma Aditi , Siddiqui Arif Jamal , Sharma Lalit
Published: May 2024

Protein kinase C (PKC) is a diverse enzyme family crucial for cell signalling in various organs. Its dysregulation is linked to numerous diseases, including cancer, cardiovascular disorders, and neurological problems. In the brain, PKC plays pivotal roles in synaptic plasticity, learning, memory, and neuronal survival. Specifically, PKC’s involvement in Alzheimer’s Disease (AD) pathogenesis is of significant interest. The dysregulation of PKC signalling has been linked to neurological disorders, including AD. This review elucidates PKC’s pivotal role in neurological health, particularly its implications in AD pathogenesis and chronic alcohol addiction. AD, characterised by neurodegeneration, implicates PKC dysregulation in synaptic dysfunction and cognitive decline. Conversely, chronic alcohol consumption elicits neural adaptations intertwined with PKC signalling, exacerbating addictive behaviours. By unravelling PKC’s involvement in these afflictions, potential therapeutic avenues emerge, offering promise for ameliorating their debilitating effects. This review navigates the complex interplay between PKC, AD pathology, and alcohol addiction, illuminating pathways for future neurotherapeutic interventions.
The Effects of Post-Exercise Cold Water Immersion on Neuromuscular Control of Knee
Authors: Wu Yuge , Qin Fanjun , Zheng Xinyan
Published: May 2024

To date, most studies examined the effects of cold water immersion (CWI) on neuromuscular control following exercise solely on measuring proprioception, no study explores changes in the brain and muscles. The aim of this study was to investigate the effects of CWI following exercise on knee neuromuscular control capacity, and physiological and perceptual responses. In a crossover control design, fifteen participants performed an exhaustion exercise. Subsequently, they underwent a 10 min recovery intervention, either in the form of passively seated rest (CON) or CWI at 15 °C. The knee proprioception, oxygenated cerebral hemoglobin concentrations (Δ[HbO]), and muscle activation during the proprioception test, physiological and perceptual responses were measured. CWI did not have a significant effect on proprioception at the post-intervention but attenuated the reductions in Δ[HbO] in the primary sensory cortex and posterior parietal cortex (p < 0.05). The root mean square of vastus medialis was higher in the CWI compared to the CON. CWI effectively reduced core temperature and mean skin temperature and improved the rating of perceived exertion and thermal sensation. These results indicated that 10 min of CWI at 15 °C post-exercise had no negative effect on the neuromuscular control of the knee joint but could improve subjective perception and decrease body temperature.
The Role of Electroencephalogram-Assessed Bandwidth Power in Response to Hypnotic Analgesia
Authors: Jensen Mark P. , Barrett Tyler D.
Published: May 2024

Research supports the efficacy of therapeutic hypnosis for reducing acute and chronic pain. However, little is known about the mechanisms underlying these effects. This paper provides a review of the evidence regarding the role that electroencephalogram-assessed bandwidth power has in identifying who might benefit the most from hypnotic analgesia and how these effects occur. Findings are discussed in terms of the slow wave hypothesis, which posits that brain activity in slower bandwidths (e.g., theta and alpha) can facilitate hypnosis responsivity. Although the extant research is limited by small sample sizes, the findings from this research are generally consistent with the slow wave hypothesis. More research, including and especially studies with larger sample sizes, is needed to confirm these preliminary positive findings.
A Comparative Analysis of the Novel Conditional Deep Convolutional Neural Network Model, Using Conditional Deep Convolutional Generative Adversarial Network-Generated Synthetic and Augmented Brain Tumor Datasets for Image Classification
Authors: Onakpojeruo Efe Precious , Mustapha Mubarak Taiwo , Ozsahin Dilber Uzun , Ozsahin Ilker
Published: May 2024

Disease prediction is greatly challenged by the scarcity of datasets and privacy concerns associated with real medical data. An approach that stands out to circumvent this hurdle is the use of synthetic data generated using Generative Adversarial Networks (GANs). GANs can increase data volume while generating synthetic datasets that have no direct link to personal information. This study pioneers the use of GANs to create synthetic datasets and datasets augmented using traditional augmentation techniques for our binary classification task. The primary aim of this research was to evaluate the performance of our novel Conditional Deep Convolutional Neural Network (C-DCNN) model in classifying brain tumors by leveraging these augmented and synthetic datasets. We utilized advanced GAN models, including Conditional Deep Convolutional Generative Adversarial Network (DCGAN), to produce synthetic data that retained essential characteristics of the original datasets while ensuring privacy protection. Our C-DCNN model was trained on both augmented and synthetic datasets, and its performance was benchmarked against state-of-the-art models such as ResNet50, VGG16, VGG19, and InceptionV3. The evaluation metrics demonstrated that our C-DCNN model achieved accuracy, precision, recall, and F1 scores of 99% on both synthetic and augmented images, outperforming the comparative models. The findings of this study highlight the potential of using GAN-generated synthetic data in enhancing the training of machine learning models for medical image classification, particularly in scenarios with limited data available. This approach not only improves model accuracy but also addresses privacy concerns, making it a viable solution for real-world clinical applications in disease prediction and diagnosis.
Socioeconomic Inequalities Affect Brain Responses of Infants Growing Up in Germany
Authors: Wienke Annika Susann , Mathes Birgit
Published: May 2024

Developmental changes in functional neural networks are sensitive to environmental influences. This EEG study investigated how infant brain responses relate to the social context that their families live in. Event-related potentials of 255 healthy, awake infants between six and fourteen months were measured during a passive auditory oddball paradigm. Infants were presented with 200 standard tones and 48 randomly distributed deviants. All infants are part of a longitudinal study focusing on families with socioeconomic and/or cultural challenges (Bremen Initiative to Foster Early Childhood Development; BRISE; Germany). As part of their familial socioeconomic status (SES), parental level of education and infant’s migration background were assessed with questionnaires. For 30.6% of the infants both parents had a low level of education (≤10 years of schooling) and for 43.1% of the infants at least one parent was born abroad. The N2–P3a complex is associated with unintentional directing of attention to deviant stimuli and was analysed in frontocentral brain regions. Age was utilised as a control variable. Our results show that tone deviations in infants trigger an immature N2–P3a complex. Contrary to studies with older children or adults, the N2 amplitude was more positive for deviants than for standards. This may be related to an immature superposition of the N2 with the P3a. For infants whose parents had no high-school degree and were born abroad, this tendency was increased, indicating that facing multiple challenges as a young family impacts on the infant’s early neural development. As such, attending to unexpected stimulus changes may be important for early learning processes. Variations of the infant N2–P3a complex may, thus, relate to early changes in attentional capacity and learning experiences due to familial challenges. This points towards the importance of early prevention programs.
Reducing Emotional Distress with Open-Label Placebos: Assessing the Role of Motor Engagement in Pill Consumption
Authors: Schienle Anne , Kogler Wolfgang
Published: May 2024

It has been posited that ingesting a pill constitutes a pivotal action that facilitates the effects of open-label placebos (OLPs: placebos honestly prescribed). In the present OLP experiment, the motor components of a placebo treatment were systematically varied. The participants (n = 183) were randomly allocated to one of four groups that all viewed aversive pictures. The ‘active OLP’ group took a placebo pill with specific instructions concerning the sequence of motor actions for the intake. The ‘usual OLP’ group swallowed the pill (without specific motor instructions), while the third group received an ‘imaginary OLP’ (no pill intake). The fourth group applied cognitive reappraisal (CR; active control group) to reduce emotional distress. The participants rated their affective state as well as the efficacy and plausibility of the treatment approach. Moreover, blood pressure and pulse were recorded as indicators of bodily arousal. The four groups did not differ in their valence ratings and physiological measures. The ‘imaginary OLP’ received higher ratings for both effectiveness and plausibility than the ‘usual OLP’. CR was rated as superior relative to all OLP conditions. In conclusion, reducing emotional distress with OLPs does not necessitate the consumption of a placebo pill. In terms of acceptability and ease of implementation, CR stands as a well-established alternative.
From Struggle to Strength: Coping with Abusive Supervision in Project Teams through Proactive Behavior and Team Building
Authors: Zhou Qiwei , Zhang Hang , Wu Qiong , Sampaio Suzana , Zouggar Anne , Cormican Kathryn
Published: May 2024

While considerable attention has been devoted to positive leadership patterns in the realm of project management, the dark side of leadership has rarely been studied within project teams. To address this gap, we focus on abusive supervision in project teams and develop a team-level moderated mediation model to examine whether, how, and when abusive supervision influences project outcomes by drawing from the Proactive Motivation Theory. Survey data were collected from 132 project teams containing 132 project managers and 392 project members using a multi-source time-lagged survey design. Our findings reveal significant negative relationships between abusive supervision and both project performance and project team creativity. Furthermore, we found that a team’s proactive behavior plays a mediating role in these relationships. More importantly, our study identifies that team building mitigates the direct negative impact of abusive supervision on proactive behavior and the indirect effects of abusive supervision on project performance and project team creativity. These findings provide valuable theoretical and managerial implications for abusive supervision and project management scholars and practitioners.
The Involvement of Academic and Emotional Support for Sustainable Use of MOOCs
Authors: Luo Zhanni , Li Huazhen
Published: May 2024

MOOCs, the Massive Open Online Courses, are online educational courses that offer open access to a large number of participants globally. However, online engagement during MOOC learning remains a problem, as reflected in relatively high dropout rates. This paper involves academic and emotional support, aiming to explore whether they contribute to users’ sustainable use of the MOOC platform. A total of 410 college students learning English as a foreign language (EFL) and with MOOC learning experience participated in this study. Employing the structural equation modeling (SEM) techniques, we examined the relationships among five factors in the EFL MOOC learning context: academic support (AS), emotional support (ES), perceived usefulness (PU), perceived ease of use (PEoU), and platform reputation (PR). The results indicate that academic support influences learners’ perceptions of the usefulness and ease of use of the MOOC platform, as well as enhancing learners’ feelings of being emotionally supported. Simultaneously, platform reputation plays a crucial role in influencing learners’ perceptions of MOOC platforms. However, results suggest that emotional support does not have a statistically significant impact on the perceived usefulness and perceived ease of use of the platform in EFL MOOC learning contexts.
Research on Planning Strategy for Urban Community Living Environment for the Elderly That Promotes “Living Mutual Aid”
Authors: Liu Tianye , Zhu Chendi , Zhou Dian , Wang Yupeng
Published: May 2024

With the development of urban population aging in China, enhancing the quality of community living environments for the elderly has become crucial. Traditional residential planning focused on functionality, neglecting the elderly’s active participation and mutual aid needs. This paper proposes the development of urban community environments promoting “living mutual aid” to improve elderly life quality and practice active aging. Using qualitative and quantitative methods, the study identifies key components of mutual aid, explores the relationship between mutual aid behaviors and spatial composition, and outlines strategies for designing community environments that support mutual aid. Based on “active aging” theory, the study qualitatively summarizes the concept of mutual aid among the elderly through literature and policy analysis. Surveys, interviews, and observations in Xi’an’s typical communities were conducted, with results analyzed using factor and frequency analysis. The study categorizes mutual aid activities and behavior characteristics, and explores the relationship between behavior and spatial needs using environmental behavior theory. It identifies mutual aid space units and suggests types, paths, strategies, and methods for integrating these units into community environments. The findings provide scientific guidance for urban community planning and valuable references for creating elderly-friendly urban habitats.
A Case Study of Air Infiltration for Highly Airtight Buildings under the Typical Meteorological Conditions of China
Authors: Du Yichen , Ji Yongming , Duanmu Lin , Hu Songtao
Published: May 2024

Passive house standard buildings (PHSBs), characterized by exceptional airtightness, present a promising technology for attaining carbon neutrality by 2060. The level of building airtightness is closely associated with air infiltration, which significantly impacts building energy consumption. However, there has been insufficient analysis of air infiltration in highly airtight buildings across diverse climatic regions. The present study involves the numerical simulation of the air infiltration rate (AIR) in an airtight building under varying design conditions during winter and summer, followed by a comprehensive analysis of the corresponding energy demand associated with air infiltration. The simulation results indicate that the building’s AIR ranges from 125 to 423 m3/h, with an average of 189 m3/h under summer design conditions, and from 40 to 344 m3/h, with an average of 198 m3/h under winter design conditions. The statistical findings demonstrate distinct distribution patterns for AIR and energy demand across various climatic regions, exhibiting significant variations in values. The discussion emphasizes the substantial heating load associated with air infiltration, even at a building airtightness level of 0.5 h−1, highlighting the necessity of considering its impact in the design of highly airtight buildings. Furthermore, it is recommended to establish specific airtightness limits for buildings in different climatic regions of China. This study offers theoretical guidance for the airtightness design of highly airtight buildings.
Research on the Method of Prestressing Tendon Layout for Large-Span Prestressed Components Continuous Rigid Frame Bridge Based on “Zero Bending Moment Dead Load Theory”
Authors: Liu Fanggang , Gu Lixiong , Fu Haishan , Li Xinping , Zhao Xiaolong , Ma Niujing , Guo Zhaolong
Published: May 2024

Based on the theory of zero bending moment under constant load, various optimization methods exist for the top beam of large-span continuous rigid frame bridges. These include achieving zero bending moment at the root of the cantilever beam, at the control stage section, and through the zero deflection method. This study aims to explore the methods and effects of optimizing roof beam design using the constant load zero bending moment method and the “three group bundle method”. Using finite element modeling, the total number and eccentricity of prestressed tendons required for each suspended pouring block are determined. Additionally, the “three group beam matching method” is employed to adjust the steel beam, adhering to the design concept of “large cantilever beam matching and small cantilever beam matching”, to achieve a reasonable configuration of the top plate beam. Through specific engineering examples, the results demonstrate that utilizing the constant load zero moment method and the “three group bundle method” can significantly enhance the structural performance and economy of large-span continuous rigid frame bridges. Moreover, it offers practical operability, providing an important reference basis for similar project designs.
Reasons for Discordance between 68Ga-PSMA-PET and Magnetic Resonance Imaging in Men with Metastatic Prostate Cancer
Authors: Wang Jade , O’Dwyer Elisabeth , Martinez Zuloaga Juana , Subramanian Kritika , Hu Jim C. , Jhanwar Yuliya S. , Nagar Himanshu , RoyChoudhury Arindam , Babich John , Huicochea Castellanos Sandra , Osborne Joseph R. , Margolis Daniel J. A.
Published: May 2024

Background: PSMA PET has emerged as a “gold standard” imaging modality for assessing prostate cancer metastases. However, it is not universally available, and this limits its impact. In contrast, whole-body MRI is much more widely available but misses more lesions. This study aims to improve the interpretation of whole-body MRI by comparing false negative scans retrospectively to PSMA PET. Methods: This study was a retrospective sub-analysis of a prospectively collected database of patients who participated in a clinical trial of PSMA PET/MRI comparing PSMA PET and whole-body MRI from 2018–2021. Subjects whose separately read PSMA PET and MRI diagnostic reports showed discrepancies (“false negative” MRI cases) were selected for sub-analysis. The cases were reviewed by the same attending radiologist who originally read the scans. The radiologist noted specific features on MRI indicating metastatic disease that were initially missed. Results: Of 263 cases, 38 (14%) met the inclusion criteria and were reviewed. Six classes of mpMRI false negatives were identified: anatomically normal (18, 47%), atypical MRI appearance (6, 16%), mischaracterization (1, 3%), undercall (6, 16%), obscured (4, 11%), and no abnormality on MRI (3, 8%). Considering that the atypical and undercalled cases could have been adjusted in retrospect, and that 4 additional cases had positive lesions to the same extent and 11 further cases had disease confined to the pelvis, only 11 (4%) of the original 263 would have had disease outside of a conventional radiation treatment plan. Conclusion: Notably, almost 50% of the cases, including most lymph node metastases, were anatomically normal using standard criteria. This suggests that current anatomic criteria for evaluating prostate cancer lymph node metastases are not ideal, and there is a need for improved criteria. In addition, 32% of cases involved some element of human interpretive error, and, therefore, improving reader training may lead to more accurate results.
Serum β-hCG as a Biomarker in Pancreatic Neuroendocrine Tumors: Rethinking Single-Analyte Approach
Authors: Komarnicki Paweł , Gut Paweł , Cieślewicz Maja , Musiałkiewicz Jan , Maciejewski Adam , Czupińska Michalina , Mastorakos George , Ruchała Marek
Published: May 2024

Despite recent advances, neuroendocrine tumors (NETs) remain a challenging topic, due to their diversity and the lack of suitable biomarkers. Multianalyte assays and the shift to an omics-based approach improve on the conventional single-analyte strategy, albeit with their own drawbacks. We explored the potential of serum β-hCG as a biomarker for NETs and discussed its role in disease monitoring. We recruited 40 patients with non-functioning pancreatic NETs, all with liver metastases. Serum β-hCG concentrations were measured at 3-month intervals over 48 months. We performed a comparative and a repeated measures analysis of β-hCG depending on WHO grade (G1, G2), liver tumor burden (LTB; below 10%, 10–25%), and RECIST 1.1. (stable disease, progressive disease). Patients with progressive disease (p < 0.001), 10–25% LTB (p < 0.001) and WHO Grade 2 (p < 0.001) displayed higher β-hCG concentrations. Throughout the study, β-hCG concentrations consistently increased across the entire cohort. Delta β-hCG during the study period was greater in patients with 10–25% LTB (p < 0.001), progressive disease (p < 0.001), and G2 (p = 0.003). Serum β-hCG correlates with established indicators of malignancy and disease progression in metastatic NETs, supporting further studies as a monitoring and prognostic biomarker. Despite promising results from novel biomarkers, there is still a place for single-analyte assays in NETs.
ARID1A Mutations in Gastric Cancer: A Review with Focus on Clinicopathological Features, Molecular Background and Diagnostic Interpretation
Authors: Angelico Giuseppe , Attanasio Giulio , Colarossi Lorenzo , Colarossi Cristina , Montalbano Matteo , Aiello Eleonora , Di Vendra Federica , Mare Marzia , Orsi Nicolas , Memeo Lorenzo
Published: May 2024

AT-rich interaction domain 1 (ARID1A) is a pivotal gene with a significant role in gastrointestinal tumors which encodes a protein referred to as BAF250a or SMARCF1, an integral component of the SWI/SNF (SWItch/sucrose non-fermentable) chromatin remodeling complex. This complex is instrumental in regulating gene expression by modifying the structure of chromatin to affect the accessibility of DNA. Mutations in ARID1A have been identified in various gastrointestinal cancers, including colorectal, gastric, and pancreatic cancers. These mutations have the potential to disrupt normal SWI/SNF complex function, resulting in aberrant gene expression and potentially contributing to the initiation and progression of these malignancies. ARID1A mutations are relatively common in gastric cancer, particularly in specific adenocarcinoma subtypes. Moreover, such mutations are more frequently observed in specific molecular subtypes, such as microsatellite stable (MSS) cancers and those with a diffuse histological subtype. Understanding the presence and implications of ARID1A mutations in GC is of paramount importance for tailoring personalized treatment strategies and assessing prognosis, particularly given their potential in predicting patient response to novel treatment strategies including immunotherapy, poly(ADP) ribose polymerase (PARP) inhibitors, mammalian target of rapamycin (mTOR) inhibitors, and enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2) inhibitors.
The Association between Family History of Lung Cancer and Development of Lung Cancer: Analysis from the KoGES Data in Korea
Authors: Kim Sang Hyuk , Lee Hyun , Kim Bo-Guen , Kim Sang-Heon , Sohn Jang Won , Yoon Ho Joo , Jang Seung Hun , Park Dong Won
Published: May 2024

Comprehensive analyses of the association between a family history of lung cancer and lung cancer risk are limited, especially in the Korean population. We used baseline data from the Korean Genome and Epidemiology Study, conducted between 2001 and 2013. This study enrolled 198,980 individuals. Lung cancer diagnoses and family histories were determined using questionnaires. Multivariable logistic regression analysis was performed to evaluate the effect of family history on the risk of lung cancer. Of 198,980 individuals, 6296 (3.2%) and 140 (0.1%) had a family history of lung cancer and lung cancer, respectively. Individuals with a family history of lung cancer in first-degree relatives (FDRs) had a higher risk of lung cancer development than those without (adjusted odds ratio [aOR] = 2.28, 95% confidence interval [CI] = 1.11–4.66). This was more pronounced in young individuals (<60 years) who had affected relatives diagnosed with lung cancer before the age of 60 years (aOR = 3.77, 95% CI = 1.19–11.88). In subgroup analyses, this association was more evident in women, never smokers, and young individuals. A family history of lung cancer, especially in FDRs, is a significant risk factor for lung cancer development in Korea.
Hu14.18K.322A Causes Direct Cell Cytotoxicity and Synergizes with Induction Chemotherapy in High-Risk Neuroblastoma
Authors: Thomas Maria , Nguyen Thu Hien , Drnevich Jenny , D’Souza Amber M. , de Alarcon Pedro A. , Gnanamony Manu
Published: May 2024

The disialoganglioside, GD2, is a promising therapeutic target due to its overexpression in certain tumors, particularly neuroblastoma (NB), with limited expression in normal tissues. Despite progress, the intricate mechanisms of action and the full spectrum of the direct cellular responses to anti-GD2 antibodies remain incompletely understood. In this study, we examined the direct cytotoxic effects of the humanized anti-GD2 antibody hu14.18K322A (hu14) on NB cell lines, by exploring the associated cell-death pathways. Additionally, we assessed the synergy between hu14 and conventional induction chemotherapy drugs. Our results revealed that hu14 treatment induced direct cytotoxic effects in CHLA15 and SK-N-BE1 cell lines, with a pronounced impact on proliferation and colony formation. Apoptosis emerged as the predominant cell-death pathway triggered by hu14. Furthermore, we saw a reduction in GD2 surface expression in response to hu14 treatment. Hu14 demonstrated synergy with induction chemotherapy drugs with alterations in GD2 expression. Our comprehensive investigation provides valuable insights into the multifaceted effects of hu14 on NB cells, shedding light on its direct cytotoxicity, cell-death pathways, and interactions with induction chemotherapy drugs. This study contributes to the evolving understanding of anti-GD2 antibody therapy and its potential synergies with conventional treatments in the context of NB.