bluuebunny/crossref_metadata_2025 huggingface dataset:
Genomic variation among closely related Vibrio alginolyticus strains is located on mobile genetic elements
Authors: Chibani Cynthia Maria , Liesegang Heiko , Roth Olivia , Wendling Carolin Charlotte
Published: February 2020

Background Species of the genus Vibrio, one of the most diverse bacteria genera, have undergone niche adaptation followed by clonal expansion. Niche adaptation and ultimately the formation of ecotypes and speciation in this genus has been suggested to be mainly driven by horizontal gene transfer (HGT) through mobile genetic elements (MGEs). Our knowledge about the diversity and distribution of Vibrio MGEs is heavily biased towards human pathogens and our understanding of the distribution of core genomic signatures and accessory genes encoded on MGEs within specific Vibrio clades is still incomplete. We used nine different strains of the marine bacterium Vibrio alginolyticus isolated from pipefish in the Kiel-Fjord to perform a multiscale-comparative genomic approach that allowed us to investigate (1) those genomic signatures that characterize a habitat-specific ecotype and (2) the source of genomic variation within this ecotype. Results We found that the nine isolates from the Kiel-Fjord have a closed-pangenome and did not differ based on core-genomic signatures. Unique genomic regions and a unique repertoire of MGEs within the Kiel-Fjord isolates suggest that the acquisition of gene-blocks by HGT played an important role in the evolution of this ecotype. Additionally, we found that ~90% of the genomic variation among the nine isolates is encoded on MGEs, which supports ongoing theory that accessory genes are predominately located on MGEs and shared by HGT. Lastly, we could show that these nine isolates share a unique virulence and resistance profile which clearly separates them from all other investigated V. alginolyticus strains and suggests that these are habitat-specific genes, required for a successful colonization of the pipefish, the niche of this ecotype. Conclusion We conclude that all nine V. alginolyticus strains from the Kiel-Fjord belong to a unique ecotype, which we named the Kiel-alginolyticus ecotype. The low sequence variation of the core-genome in combination with the presence of MGE encoded relevant traits, as well as the presence of a suitable niche (here the pipefish), suggest, that this ecotype might have evolved from a clonal expansion following HGT driven niche-adaptation.
Circ_0015756 aggravates hepatocellular carcinoma development by regulating FGFR1 via sponging miR-610
Authors: Guo Weisheng , Zhao Lin , Wei Yaguang , Liu Peng , Zhang Yu , Fu Liran
Published: February 2020

Background: Hepatocellular carcinoma (HCC) is the leading threat of cancer-related death in humans with poor therapeutic effects. Circular RNAs (circRNAs) are important indicators in cancer diagnosis and prognosis. This study intended to explore the function and mechanism of circ_0015756 in HCC, providing the additional opinion for HCC treatment.Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was utilized to detect the expression of circ_0015756 and miR-610. Cell viability was assessed by cell counting kit-8 (CCK-8) assay, and colony formation capacity was ascertained by colony formation assay. Cell proliferation and invasion were monitored by transwell assay. Cell cycle progression and apoptosis were analyzed by flow cytometry assay. Circ_0015756 oncogenicity was determined by Xenograft models. The prediction of targets was performed using the bioinformatics tools, and the verification of targeted relationship was conducted using RNA pull-down, RNA immunoprecipitation (RIP) and dual-luciferase reporter assays. The expression level of fibroblast growth factor receptor 1 (FGFR1) was measured by western blot.Result: The expression of circ_0015756 was increased in HCC tissues, serums and cells. Circ_0015756 downregulation impaired HCC cell viability, colony formation capacity, invasion and migration, induced cell cycle arrest and apoptosis, and inhibited tumor growth in vivo. MiR-610 was ensured as a target of circ_0015756, and miR-610 absence reversed the effects of circ_0015756 downregulation. Further, FGFR1 was interacted by miR-610, and FGFR1 overexpression overturned the effects of miR-610 restoration in vitro. Circ_0015756 could regulate FGFR1 expression by targeting miR-610.Conclusion: Circ_0015756 played its tumorigenic properties in HCC by activating FGFR1 and sponging miR-610, and circ_0015756 was expected to be a vital indicator in HCC diagnosis and treatment.
Kartogenin mediates cartilage regeneration via stimulating the IL-6/Stat3-dependent proliferation of cartilage stem/progenitor cells
Authors: Liu Tao , Li Xiaolin , Wang Ting , Zhang Shuai , Zhu Yanxia , Chen Xuemei , Zhou Meiling , Liao Jinqi , Du Jie , Wei Pengfei , Huang Yongcan , Wang Wenxin , Chen Junhui , Zou Xuenong , Zhou Guangqian
Published: February 2020

Background: Declination of endogenous stem cells in cartilage is regarded as the cause of cartilage degeneration. Kartogenin (KGN) is well known to play an important role in chondrogenesis of mesenchymal stem cells (MSC).Methods: Using MSCs isolated from rat cartilages, we analyzed the changing of transcriptomics after the treatment of KGN in vitro. DMM animal models were then applied to identified the effect of MSCs proliferation in vivo after KGN capsule injection. Furthermore, we explored the potential mechanisms how KGN mediates cartilage regeneration and proliferation of cartilage progenitor cells.Results: In this study, we demonstrate that KGN can promote the proliferation of MSC from cartilage, respectively. The percentage of G2-M phase cells in culture reached over 10%, nearly twice as the control group with KGN treatment. Transcriptomic profiling of rat cartilage stem/progenitor cells (CSPC) revealed that the expression of at least 20 cell cycle related genes was significantly changed in response to the KGN treatment. IL-6 and its co-receptor Gp130 gene expression level are much higher than the untreated control. The phosphorylation of the IL-6-downstreamt molecular Stat-3 was enhanced upon the KGN stimulation. The knee joint injury animal model further showed the increased articular cartilage thickness after KGN treatment. Interestingly, the IHC staining also demonstrated the up-regulated level of Stat-3 phosphorylation and enhanced distribution of CD44+/CD105+ cells in the KGN-treated cartilage.Conclusion: Taken together, our data suggest that KGN promotes cartilage regeneration at least partially by stimulating the IL-6/Stat3-dependent proliferation of stem cells resident in the cartilage.
SIRT6-Autophagy-Warburg effect axis in papillary thyroid cancer
Authors: Yang Zhou , Huang Renhong , Yu Weiping , Min Zhijun , Ye Min
Published: February 2020

Background: Our previous study has demonstrated SIRT6 promotes aggression and epithelial-mesenchymal transition (EMT) in papillary thyroid cancer (PTC). In this study, we focused on the regulatory axis between SIRT6-Autophagy-Warburg effect.Methods: Autophagy activation was confirmed by Western Blotting, quantitative PCR, immunofluorescence and transmission electron microscopy. Warburg effect was examined by Seahorse XF96 analysis and PET imaging. Regulation of SIRT6 in negatively regulates reactive oxygen species (NRROS) was confirmed by chromatin immunoprecipitation.Results: We innovatively confirmed overexpression of SIRT6 depleted histone H3 lysine 56 acetylation (H3K56ac) of NRROS in vitro, thus increased reactive oxygen species (ROS). Then, accumulated ROS activated endoplasmic reticulum stress (ER stress), and subsequently induced autophagy. Furthermore, the overexpression of SIRT6 inhibited Glucose Transporter 1 (Glut1) via autophagic degradation, thus ulteriorly suppressed Warburg effect. Treatment of ROS scavenger N-acetyl-L-cysteine (NAC, 5mM) or autophagy inhibitor chloroquine (CQ) both rescued the inhibition of Warburg effect. In addition, higher concentration of NAC (15mM) deepened the inhibited Warburg effect. This concentration-dependent bilateral effects of NAC in this process had been confirmed owe to regulation of AMPK signaling pathway. Finally, we further determined above mechanism in vivo via subcutaneous xenografts in nude mice applied with 18F-FDG PET/CT.Conclusions: We identified a SIRT6-ROS-ERstress-Autophagy-Glut1-Warburg effect axis in PTC, which may provide new target for therapy. In addition, NAC (low concentration) and CQ which previously been considered as tumor inhibitors, have been shown to promote tumorigenesis in PTC with high SIRT6 expression via activation of Warburg effect.
Association between diet quality and food waste in Canadian families: a cross-sectional study
Authors: Carroll Nicholas , Wallace Angela , Jewell Kira , Darlington Gerarda , Ma David W.L. , Duncan Alison M. , Parizeau Kate , von Massow Michael , Haines Jess
Published: February 2020

Background: Higher diet quality has been associated with greater amounts of food waste among adults in the United States. This study aims to build on previous work by examining the association between diet quality and food waste, as assessed using detailed waste audits, among a sample of Canadian families.Methods: This cross-sectional study used data from 85 Canadian families with young children. Parent and children diet quality was assessed using the Healthy Eating Index-2015 (HEI-2015), calculated from 3-day food records. Household food waste was measured using detailed waste audits conducted over multiple weeks and these data were used to calculate daily per capita food waste. Linear regression was used to explore the association between parent and child HEI-2015 scores and daily per capita total avoidable and unavoidable food waste, as well as daily per capita avoidable and unavoidable food waste in the following categories: 1) fruits and vegetables, 2) milk, cheese and eggs, 3) meat and fish, 4) breads and cereals, 5) fats and sugars.Results: Parent HEI-2015 scores ranged from 37 to 92 (out of 100) and 81% of parents’ diets scored in the “Needs Improvement (50-80)” category. Parent and child diet quality scores were significantly correlated (r=0.61; P<0.0001) and 82% of children’s diets scored in the “Needs Improvement” category. On average, households produced 107 grams of avoidable food waste and 52 grams of unavoidable food waste per person per day. Fruits and vegetables were the highest contributor for both avoidable and unavoidable food waste. Both parent and child HEI-2015 scores were not significantly associated with total daily per capita avoidable or unavoidable food waste. However, parent HEI-2015 scores were positively associated with daily per capita avoidable fruit and vegetable waste (β= 1.05; 95%CI: 0.11, 1.99; P= 0.03) and daily per capita unavoidable fruit and vegetable waste (β= 0.60; 95%CI: 0.03, 1.17; P= 0.04), after adjusting for household income.Conclusion: This is the first study to explore the association between diet quality and food waste using detailed waste audits. Future research should explore effective strategies towards improving diet quality while simultaneously reducing food waste, especially of fruits and vegetables.
Molecular Epidemiology of Pulmonary Tuberculosis in Southern Ethiopia
Authors: Merid Yared , Hailu Elena , Habtamu Getnet , Tilahun Melaku , Abebe Markos , Hailu Mesay , Hailu Tsegaye , Datiko Daniel G. , Woldeamanuel Yimtubezinash , Aseffa Abraham
Published: February 2020

Background Understanding the epidemiology of tuberculosis (TB) is limited by lack of genotyping data. We sought to characterize the drug susceptibility testing (DST) patterns and genetic diversity of M. tuberculosis (Mtb) isolates in Southern Ethiopia. Methodology A cross-sectional study was conducted among newly diagnosed sputum smear positive patients with TB visiting nine health facilities in southern Ethiopia from June 2015 to May 2016. Three consecutive sputum samples (spot-morning-spot) per patient were examined using acid-fast bacilli (AFB) smear microscopy with all smear positive specimens having AFB cultures performed. Mtb isolates had DST performed using indirect proportion method and were genotyped with RD9 deletion typing and spoligotyping. Spoligotyping International Types (SIT) and sub-lineages (clades) were assigned according to the SITVITWEB data base. Results Among 250 newly diagnosed patients with TB, 154 (52%) were male and 143 (57%) from rural areas. The prevalence of HIV co-infection was 4%. Of the 250 AFB positive sputum samples, 230 (92%) were culture positive. All 230 isolates were M. tuberculosis strains belonging to three lineages: Euro-American, 187 (81%); East-African-Indian, 31 (14%); and Lineage 7 (Ethiopian lineage), 8 (4%). The 230 isolates could be categorized into 65 different spoligotype patterns, of which 84% fell into 29 clusters. The dominant spoligotypes were SIT149 (21%), SIT53 (19%) and new strains (16%). Mtb strains were clustered by districts. DST revealed that 14% of Mtb isolates were resistant to > 1 first line anti-TB drugs including 11% to isoniazid. SIT 149 was the most prevalent genotype among drug resistant isolates (20%). Conclusion The study revealed several clusters including lineage 7 strains circulating in southern Ethiopia. SIT 149 (T3-ETH) was the most dominant circulating strain in the study area including among drug-resistant cases.
Non-Invasive Oxygenation Strategies in Adult Patients with Acute Respiratory Failure: A Protocol for a Systematic Review and Network Meta-analysis
Authors: Ferreyro Bruno Leonel , Angriman Federico , Munshi Laveena , Sorbo Lorenzo del , Ferguson Niall D , Rochwerg Bram , Ryu Michelle , Saskin Refik , Wunsch Hannah , Costa Bruno R da , Scales Damon C
Published: February 2020

Background: Acute hypoxemic respiratory failure is one of the leading causes of intensive care unit admission and associated with high mortality. Non-invasive oxygenation strategies such as high flow nasal cannula, standard oxygen therapy and non-invasive ventilation (delivered by either face mask or helmet interface) are widely available interventions applied in these patients. It remains unclear which of these interventions are more effective in decreasing rates of invasive mechanical ventilation and mortality. The primary objective of this network meta-analysis is to summarize the evidence and compare the effect of non-invasive oxygenation strategies on mortality and need for invasive mechanical ventilation in patients with acute hypoxemic respiratory failure. Methods: We will search key databases for randomized controlled trials assessing the effect of non-invasive oxygenation strategies in adult patients with acute hypoxemic respiratory failure. We will exclude studies in which the primary focus is either acute exacerbations of chronic obstructive pulmonary disease or cardiogenic pulmonary edema. The primary outcome will be all-cause mortality (longest available up to 90 days). The secondary outcomes will be receipt of invasive mechanical ventilation (longest available up to 30 days). We will assess the risk of bias for each of the outcomes using the Cochrane Risk of Bias Tool. Bayesian network meta-analyses will be conducted to obtain pooled estimates of head-to-head comparisons. We will report pairwise and network meta-analysis treatment effect estimates as risk ratios and 95% credible intervals. Subgroup analyses will be conducted examining key populations including immunocompromised hosts. Sensitivity analyses will be conducted by excluding those studies with high risk of bias and different etiologies of acute respiratory failure. We will assess certainty in effect estimates using GRADE methodology. Discussion : This study will help to guide clinical decision making when caring for adult patients with AHRF and improve our understanding of the limitations of the available literature assessing noninvasive oxygenation strategies in acute hypoxemic respiratory failure.
Assessment of Quality of life Among Children with Cancer, Children Undergoing Hemodialysis and Children with Thalassemia: A Comparative Study
Authors: Afifi Tayseer , Elessi Khamis , Baraka Obay , Omar Mohammed , Ahmad Israa , Al-Attar Hala , Qudaih Ameera , Alsultan Khalid , Abdelati Meral , Astal Alaa , Shammala Samar Abu , Obaid Mohammed , Abu-Rous Mohammed , Samaan Mosab , Albatsh Khalil , Almoqaid Abdarrahman
Published: February 2020

Purpose This study aims to evaluate health-related quality of life among children with different chronic conditions and make a comparison among them. In addition, the study will draw a comparison between children’s perspective about their quality of life and parents’ perspective. Furthermore, the study will address the associations between quality of life with disease severity, duration and the presence of other co-morbidities. Methods This was a prospective, analytical, correlational study design in which Pediatric Quality of Life Inventory (PedsQL 4.0 generic core scale) was administered to assess quality of life on 110 children; 50 children with cancer, 30 children with thalassemia and 30 children undergoing hemodialysis. Researchers interviewed children as well as their parents. Results Results showed that children with Thalassemia had the lowest scores and suffered from poor quality of life compared to the other two groups of children. Interestingly, quality of life level among children undergoing dialysis was lower than children with cancer. However, there were marked discrepancy between child’s and parents’ answers on scale questions. The level of quality of life, in all children, were statistically significant in relation with family size, income and parents’ education. Conclusion We identified high prevalence of poor level of health-related quality of life among children included in this study. The findings support that the quality of life among children with chronic conditions should receive more attention in our local medical settings.
A model for evaluation of the electric activity and oxygenation in the erector spinae muscle during isometric loading adapted for spine patients
Authors: Ekström Lars , Zhang Qiuxia , Abrahamson Josefin , Beck Joel , Johansson Christer , Westin Olof , Todd Carl , Baranto Adad
Published: February 2020

Background: Simultaneous measurement of electromyography (EMG) and local muscle oxygenation is proposed in an isometric loading model adjusted for patients that have undergone spinal surgery.Methods: Twelve patients with degenerative lumbar spinal stenosis (DLSS) were included. They were subjected to a test protocol before and after surgery. The protocol consisted of two parts, a dynamic and an isometric Ito loading with a time frame of 60 seconds and accompanying rest of 120 seconds. The Ito test was repeated three times. EMG was measured bilaterally at the L4 level and L2 and was recorded using surface electrodes and collected (Biopac Systems Inc). EMG signal was expressed as RMS and median frequency (MF). Muscle tissue oxygen saturation (MrSO 2 ) was monitored using a Near-infrared spectroscopy (NIRS) device ( INVOS ® 5100C Oxymeter). Two NIRS sensors were positioned bilaterally at the L4 level. The intensity of the leg and back pain and perceived exertion before, during and after the test was evaluated with a Visual Analogue Scale (VAS) and Borg RPE-scale, respectively.Results: All patients were able to perform and complete the test protocol pre- and postoperatively. A consistency of lower median and range values was noted in the sensors of EMG1 (15.3 µV, range 4.5-30.7 µV) and EMG2 (13.6 µV, range 4.0 -46.5 µV) that were positioned lateral to NIRS sensors at L4 compared with EMG3 (18.9 µV, range 6.5 -50.0 µV) and EMG4 (20.4 µV, range 7.5 -49.0 µV) at L2. Right and left side of the erector spinae exhibited a similar electrical activity behaviour over time during Ito test (60s). Regional MrSO 2 decreased over time during loading and returned to the baseline level during recovery on both left and right side. Both low back and leg pain was significantly reduced postoperatively.Conclusion: Simultaneous measurement of surface EMG and NIRS seems to be a promising tool for objective assessment of paraspinal muscle function in terms of muscular activity and local muscle oxygenation changes in response to isometric trunk extension in patients that have undergone laminectomy for spinal stenosis.
How much total ovarian tissue can be removed without compromising ovarian function? An animal study
Authors: Cheng Jiaojiao , Ruan Xiangyan , Zhou Qi , Li Yanglu , Du Juan , Jin Fengyu , Wang Husheng , Gu Muqing , Mueck Alfred Otto
Published: February 2020

Purpose Until now, it is not clear how much total ovarian tissue can be removed without affecting ovarian functions. Aim to investigate impact of stepwise removed ovarian tissue on endocrine hormone levels reflecting ovarian function, follicle morphology and weight in rats. Methods The study was performed with six groups of animals, each consisting of six ten-week-old Sprague-Dawley rats. A blood test was performed on the day of surgery (baseline) and fortnightly thereafter to assess serum hormones reflecting ovarian function: estradiol (E2), progesterone (P), follicle-stimulating hormone (FSH), anti-Müllerian hormone (AMH), inhibin B (INHB); weight was also assessed. The ovaries obtained from surgical resection and from the rats sacrificed 12 weeks after surgery were stained with hematoxylin-eosin. Surgeries in the groups; [%] of remaining total ovarian tissue: Group 1, non-ovariectomized [100% tissue]. Group 2, half of left ovary excised [75% tissue]. Group 3, left ovary excised [50% tissue]. Group 4, left ovary + half of right ovary excised [25% tissue]. Group 5, left ovary + three quarters of right ovary excised [12.5% tissue]. Group 6, bilateral ovariectomy [0% tissue]. Results During the 12 weeks, group 4, 5 and 6 had higher FSH and lower AMH and INHB values compared to the control (group 1) ( p <0.05), but in group 4 production of E2 and P was not significantly different from control ( p >0.05). All ovarian function parameters completely stopped in group 5, i.e. the same as the bilateral ovariectomized rats (group 6). Weight significantly increased in group 6 ( p <0.05). Follicle morphology at baseline and 12 weeks after surgery in groups 2-5 was not significantly different to the control. Conclusions Our animal study confirms that removal of one ovary maintains the remaining ovarian tissue's potency for normal hormone production, as already suggested in clinical studies. However, to our knowledge, we demonstrate here for the first time that even up to 75% of total ovarian tissue can be removed without impact on E2 and P production, which if confirmed in women would mean that hazardous hormone replacement therapy is not required to avoid the negative consequences of hormone depletion.
The Effect of Glutamine on Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Rats
Authors: Wu Gengxiang , Hu Xue , Ding Jinli , Yang Jing
Published: February 2020

Background Previous studies have shown that chronic inflammation and oxidative stress may play an important role in the pathophysiology of polycystic ovary syndrome (PCOS), and glutamine (Gln) showed the anti-inflammatory and antioxidant properties. So the aim of this study is to investigate the effect of glutamine supplementation on PCOS rats. Methods Female Sprague–Dawley rats were randomly assigned into five groups (n = 10 /group), control group, PCOS group, PCOS+0.5g/kg Gln group, PCOS+0.75g/kg Gln group and PCOS+1.0g/kg Gln group. All the PCOS rats were administrated with 6 mg/100 g dehydroepiandrosterone (DHEA) for 20 consecutive days, all the PCOS+Gln groups were intraperitoneal injected Gln twice in the next morning after the last DHEA injection. All the samples were collected 12 hours after the last administration. Ovarian histological examinations were analyzed and the concentration of serum hormone, inflammatory and oxidative stress factors were measured. Results There was no obvious ovarian histological change among the PCOS group and PCOS+Gln groups. All the detected inflammation factors (IL-6, IL-18, TNF-α and CRP) showed significantly higher in all the PCOS groups compared to the control group (P<0.01), and were significantly decreased with the low supplementation of glutamine (P<0.01, include 0.5g/kg group and 0.75g/kg group).Concentrations of SOD were significantly lower in all the PCOS groups (P<0.01) compared to the control group, and increased significantly with the low supplementation of glutamine (P<0.01, include 0.5g/kg group and 0.75g/kg group). Serum concentrations of MDA, NO and NOS were significantly higher in PCOS group (P<0.01) compared with the control group, and significantly decreased to the comparative levels of control group with the low supplementation of glutamine (P<0.01, include 0.5g/kg group and 0.75g/kg group). Conclusion There is low-grade inflammation and oxidative stress in DHEA-induced PCOS rats. The supplementation of glutamine with doses of 0.5g/kg and 0.75g/kg could effectively ameliorate the inflammatory and oxidative stress conditions of PCOS.
Back protection of canvas paintings
Authors: Padfield Tim , Padfield Nicolas , Lee Daniel Sang-Hoon , Thøgersen Anne , Nielsen Astrid Valbjørn , Andersen Cecil Krarup , Scharff Mikkel
Published: February 2020

A painting on canvas, stretched on a wooden frame, was fitted with various styles of back protection and then exposed to a cycle of temperature variation at the back, with the front exposed to a constant room temperature. The painting was also exposed to a constant wall temperature and varying room temperature. The space between the canvas and the back board was fitted with temperature and relative humidity (RH) sensors. The unprotected painting suffered a large RH variation at its back, because of the varying canvas temperature interacting with the constant room air moisture content. Effective stabilisation of the RH behind the canvas against temperature variation was provided by a shiny aluminium alloy sheet sealed against the frame. The non-absorbent back board experienced a strong variation in RH, because of humidity buffering of the space by the painting canvas at a different temperature. Either a space or insulation between this back plate and the wall reduced the risk of condensation on the inner surface of the back plate. Insulation will however increase the risk of condensation on the wall surface behind the painting. An absorbent back board de-stabilised the RH at the painting canvas surface by providing a competing humidity buffer at a different temperature. To provide protection against moisture exchange with an unsuitable room RH, extra humidity buffer was placed 3 mm behind the painting canvas, kept close to the painting temperature by insulation between this buffer and the back board. This stabilised RH at the canvas surface but increased both the temperature and the RH variation at the back board and thus increased the risk of condensation on the inner surface of the back board. The RH and the temperature in the narrow spaces between the painting canvas and the wooden stretcher frame were always more nearly constant than in the open canvas area, which suggests an explanation for the widely observed better condition of the areas of canvas paintings which lie close over the support structure. Our conclusion is that a non-absorbent, impermeable back plate gives good RH stability against a changing temperature gradient between wall and painting surface.
Estimating the Risk of Declining Funding for Malaria in Ghana: The case for continued investment in the malaria response
Authors: Shretta Rima , Silal Sheetal P , Malm Keziah , Mohammed Wahjib , Narh Joel , Piccinini Danielle , Bertram Kathryn , Rockwood Jessica , Lynch Matt
Published: February 2020

Background: Ghana has made impressive progress against malaria, decreasing mortality and morbidity by over 50% between 2005-2015. These gains have been facilitated in part, due to increased financial commitment from both government and donors. Between 2006 and 2011, total resources for malaria increased from less than USD 25 million in 2006 to over USD 100 million. However, the country still faces a high burden of disease and is at risk of declining external financing due to its strong economic growth and the consequential increased donor requirements for domestic contributions. The resulting financial gap will need to be met domestically to accelerate progress. The purpose of this study was to provide evidence of the economic impact of malaria elimination and the potential risks of withdrawing financing to shape an advocacy strategy for resource mobilization.Methods: A compartmental transmission model was developed to estimate the impact of a range of malaria interventions on the transmission of Plasmodium falciparum malaria between 2018 and 2030. The model projected scenarios that allowed the attainment of elimination using a package of common interventions and scenarios that predicted transmission if interventions were withheld. The outputs of this model were used to generate costs and economic benefits of each option.Results: Elimination was predicted using the interventions outlined in the national strategy, particularly increased net usage and improved case management. Malaria elimination in Ghana was predicted cost USD 961 million between 2020 and 2029. Compared to the baseline, elimination is estimated to prevent 85.5 million cases, save 4,468 lives, and avert USD 2.2 billion in health system expenditures. The economic gain was estimated at USD 32 billion in reduced expenditure, increased household prosperity and productivity gains. Through malaria elimination, Ghana can expect to see a 32-fold return on their investment. Withdrawing interventions, predicted an additional 38.2 clinical cases, 2,500 deaths and additional economic losses of USD 14.1 billion.Conclusions: Although government financing has increased in the past decade, the amount is less than 25% of total malaria financing. The study findings can be used to develop a robust strategy to overcome financial barriers for malaria elimination in Ghana.
The impact of macroeconomics factors on real exchange rate in Latin America: A dynamic panel data analysis
Authors: Chavez Carlos
Published: February 2020

This paper study the determinants of real exchange rate from an internal perspective using macroeconomic variables that can be endogenous. We use a GMM System estimator and transform our variables using forward orthogonal deviations (FOD) and first differences (FD). For check the robustness of our estimations, we use different sizes T. Our results show that terms of trade have negative and significant impact for all sizes. Th gross domestic product has positive and significant impact and the rest of variables has mixed effects depending the sizes T.
The role of swnR gene on the biosynthesis pathway of the swainsonine in Metarhizium anisopliae
Authors: Sun Lu , Song Runjie , Wang Jinglong , Zhang Yu , Zhu Yanli , Li Bo , Liu Yiling , Zhao Wenxing , Mo Chonghui , Guo Qingyun , Zhao Baoyu , Lu Hao
Published: February 2020

Background: Swainsonine (SW) is the principal toxic ingredient of locoweeds, and is produced by fungi including Metarhizium anisopliae, Slafractonia leguminicola, and Alternaria oxytropis (found in locoweeds of Oxytropis). Studies of the SW biosynthesis pathway in these fungi have demonstrated the requirement for a swnK gene and the presence of a variety of other SWN cluster genes, but have not determined a precise role for the swnR gene, which encodes a NADB Rossmann-fold reductase, nor if it is necessary for the biosynthesis of SW. In this study, we used homologous recombination (HR) to knock out the swnR gene of M. anisopliae to determine its effect on the SW biosynthesis pathway.Results: The concentration of SW was measured in the fermentation broth of M. anisopliae at 1 d, 3 d, 5 d and 7 d using a Q Exactive Mass Spectrometer. The gene for swnR was detected by RT-qPCR. To determine the role of the swnR gene in the SW biosynthesis pathway of M. anisopliae, we used PEG-mediated homologous recombination (HR) to transform a wild-type strain (WT) with a Benomyl (ben)-resistant fragment to knock out the swnR gene producing a mutant-type strain (MT). A complemented-type (CT) strain was produced by adding a complementation vector that contains the glufosinate (herbicide) resistance (bar) gene as a marker. The content of SW decreased, but was not eliminated in the fermentation broth of the MT strain, and returned to the original level in the CT strain.Conclusions: These results indicate that the swnR gene plays a crucial role in the SW biosynthesis pathway of M. anisopliae, but suggests that another gene in the fungus may share the function of swnR.
The role of animals as a source of antimicrobial resistant nontyphoidal Salmonella causing invasive and non-invasive human disease in Vietnam
Authors: Parisi Andrea , Phuong Tu , Mather Alison E , Jombart Thibaut , Tuyen Ha Thanh , Lan Nguyen Phu Huong , Trang Nguyen Hoang Thu , Carrique-Mas Juan , Campbell James I , Trung Nguyen Vinh , Glass Kathryn , Kirk Martyn D , Baker Stephen
Published: February 2020

BackgroundNontyphoidal Salmonella (NTS) are associated with both diarrhea and bacteremia. Antimicrobial resistance (AMR) is common in NTS in low-middle income countries, but the major source(s) of AMR NTS in humans are not known. Here, we aimed to assess the role of animals as a source of AMR in human NTS infections in Vietnam. We retrospectively combined and analyzed 672 NTS human and animal isolates from four studies in southern Vietnam and compared serovars, sequence types (ST), and AMR profiles. We generated a population structure of circulating organisms and aimed to attribute sources of AMR in NTS causing invasive and noninvasive disease in humans using Bayesian multinomial mixture models.ResultsAmong 672 NTS isolates, 148 (41%) originated from human blood, 211 (59%) from human stool, and 136 (43%) from animal stool. The distribution of serovars, STs, and AMR profiles differed among sources; serovars Enteritidis, Typhimurium, and Weltevreden were the most common in human blood, human stool, and animals, respectively. We identified an association between the source of NTS and AMR profile; the majority of AMR isolates were isolated from human blood (p<0.001). Modelling by ST-AMR profile found chickens and pigs were likely the major sources of AMR NTS in human blood and stool, respectively; but unsampled sources were found to be a major contributor.ConclusionsAntimicrobial use in food animals is hypothesized to play role in the emergence of AMR in human pathogens. Our cross-sectional population-based approach suggests a significant overlap between AMR in NTS in animals and humans, but animal NTS does explain the full extent of AMR in human NTS infections in Vietnam.
Rare partial trisomy and tetrasomy of 15q11-q13 associated with developmental delay and autism spectrum disorder
Authors: Liang Yi , Lu Yinghong , Ning Sisi , Deng Guosheng , Xie Yulin , Song Jujie , Zuo Na , Feng Chunfeng , Qin Yunrong
Published: February 2020

Background: Small supernumerary marker chromosomes (sSMCs), are additional abnormal chromosomes, which can’t be detected accurately by conventional cytogenetic analysis. Duplication of chromosome 15 might be account for 50% of the total sSMCs and usually leads to mental retardation, structural malformation, behavioral problems and epilepsy.Case presentation: An 11-month-old infant with an sSMC was referred to our clinic because of developmental retardation and autism spectrum disorder. After several months of rehabilitation treatment, the progress of motor development was obvious, but the consciousness was still far from satisfied. High-resolution karyotype analysis, multiplex ligation-dependent probe amplification and copy number variation sequencing (CNV-Seq) were conducted to confirm the identity of the sSMC. A bisatellited dicentric sSMC was observed clearly in high-resolution karyotype analysis and a 10.16-Mb duplication of 15q11.1q13.2 together with a 1.84-Mb duplication of 15q13.2q13.3 was showed by CNV-Seq in the proband. It suggested that the molecular cytogenetic karyotype was 47, XY,+inv dup(15)(pter/q13::q13/pter) which identify the proband was 15q duplication syndrome (dup15q). Furthermore, the clinical symptoms of the proband mostly fit this disease which is characterized by hypotonia motor delays, autism spectrum disorder (ASD), and intellectual disability.Conclusion: Our research indicates that CNV-seq is a robust, sensitive and economical way for diagnosis of dup15q and related disorders.
Influence of high-heeled shoe parameters on biomechanical performance of young female adults during stair ascent motion
Authors: shang jiangyinzi , Zhang Shuiqiang , yan alan , ma xin
Published: February 2020

Background: No prior work has compared the biomechanical workload of stair ascent (SA) using shoes of different heel heights and heel types. Therefore, this study aimed to investigate the influence of high-heeled shoe (HHS) parameters on pelvis position, lower extremities kinematics, ground reaction force, and intern stress in young women during SA motion.Methods: The participants were instructed to ascend a 3-step staircase, wearing HHSs of different heel heights and heel types, and the control group used a pair of flat shoes. By using finite element methodology, we investigated the influence of shoe parameters on metatarsal stress and metatarsal head loading during SA.Results: As the heel height increased, increased ranges of ankle dorsiflexion-plantarflexion and pelvic rotation were observed. A thinner heel type displayed a larger pelvic forward tilt movement and 1st peak vertical force, as well as a smaller 2nd peak vertical force. With higher heels, increased external rotation of the knee, inversion and plantar flexion, and flexion values of the knee were observed. Meanwhile, there was decreased external rotation of the pelvis, ankle eversion, varus, and dorsiflexion. Three major stance phases namely heel strike, midstance, and push off were simulated to investigate the biomechanical response of high-heeled walking. It was found that the contact pressure at plantar pressure and 1st metatarsal pressure intensified with flat shoes and reached their maximum at push off phase during locomotion.Conclusion: To stabilize body posture during SA when wearing HHSs with a small heel base area, compensatory changes to drive an effective motor response include increasing pelvic range of motion in the transverse plane and sagittal plane, changing the joint angles of the lower extremities, and increasing metatarsal intern stress, may lead to clinical symptoms.
The variation of pelvis in unilateral Crowe type IV developmental dysplasia of the hip
Authors: Du Yinqiao , Sun Jingyang , Ma Haiyang , Shen Junmin , Ni Ming , Zhou Yonggang
Published: February 2020

BackgroundThe whole pelvic morphology is very common in developmental dysplasia of the hip (DDH). The abnormalities may influence the pelvic landmarks, and then misguide the surgeon’s preoperative plan. The purpose of this study was to investigate the variation of pelvis in unilateral Crowe type IV DDH and analyze the reliability of pelvic landmarks.MethodsWe received preoperative anteroposterior pelvic radiographs examined for 89 adult patients with unilateral Crowe type IV DDH at our institution between September 2008 and May 2019. 48 patients without a false acetabulum was type IVA and 41 with a false acetabulum was type IVB. The heights of the ilium, acetabulum and ischium areas in affected and unaffected sides were measured, the discrepancies of bilateral iliac crest, inferior sacroiliac articulation, teardrop and ischial tuberosity on the bisector of the pelvis were also measured.ResultsThe mean heights of the ilium, acetabulum, ischium areas in the affected side were 74.4 mm, 88.6 mm and 37.0 mm, respectively, in type IVA group and 77.7 mm, 83.5 mm and 37.8 mm, respectively, in type IVB group. The heights in the unaffected side were 82.1 mm, 84.6 mm and 43.8 mm, respectively, in type IVA group and 84.6 mm, 82.0 mm and 44.0 mm, respectively, in type IVB group. The discrepancies of bilateral iliac crest, inferior sacroiliac articulation, teardrop and ischial tuberosity on the line of the bisector of the pelvis were 6.7 mm, 4.4 mm, 2.7 mm and 6.1 mm, respectively.ConclusionsThe pelvic asymmetry was a common occurrence in adults unilateral Crowe type IV DDH. Furthermore, it should be reliable to use teardrop as pelvic landmark to balance leg length discrepancy in preoperative planning.
Selenium ameliorates S. aureus-induced inflammation through ROS-mediated NLRP3 inflammasome in bovine mammary epithelial cells
Authors: Bi Chongliang , Zhang Shujiu , Tang He , Li Hui
Published: February 2020

Background Some research has indicated that selenium (Se) plays a significant role during mastitis. However the intracellular anti-inflammatory effect of Se is not fully clear. Due to the ability of Staphylococcus aureus ( S. aureus ) to internalize into host cell, in this study we explored whether Se could regulate inflammation induced by S. aureus through reactive oxygen species (ROS)-mediated NLRP3 inflammasome in bMECs. Result bMECs were treated with 8 μmol/L Na 2 SeO 3 for 12 h before infected with S. aureus for 2 h. Through flow cytometry, Western blot and qPCR analysis, ROS and NLRP3 imflammasome were detected. Result shown that the production of ROS was increased by S. aureus , Se exerted strong inhibitory effects on the production of ROS; The protein expression of NLRP3 inflammasome including NLRP3, ASC and Caspase-1 increased significantly after S. aureus infection, Se played an important role in regulating the expression of NLRP3, ASC and Caspase-1; To further investigate the anti-inflammatory effect of Se, the expression level of IL-1β associated molecule pro-IL-1β and IL-1β were detected. Result shown that the mRNA expression of IL-1β was up-regulated by S. aureus and after Se treatment the expression level of IL-1β mRNA was markedly down-regulated, meanwhile Se play a regulation effect on the protein expression of Pro-IL-1β and IL-1β. Conclusions Here we show that ROS is involved in bMECs inflammation induced by S. aureus and Se ameliorates S. aureus -induced inflammation through ROS-mediated NLRP3 pathway in bMECs.
The dynamic mutation investigation and whole exome sequencing in a cohort of Chinese autosomal dominant cerebellar ataxia patients
Authors: Peng Fang , Zhang Yue , Zhou Xin-Yue , Huang Shuai-Qi , Chen Chen , Ding Zheng-Tong , Wang Jian , Sun Yi-Min , Wu Jianjun
Published: February 2020

Background Spinocerebellar ataxias (SCAs) are the autosomal dominant cerebellar ataxia (ADCA) with great clinical and genetic heterogeneity. Genetic testing will contribute to the final diagnosis. Methods A total of 204 Chinese ADCA patients were recruited and 190 had genetic testing. Dynamic mutations of SCA1, 2, 3, 6, 7, 8, 10, 12, 17 and dentatorubral-pallidoluysian atrophy (DRPLA) were screened firstly. For the patients with negative results, the dynamic mutations of HTT of Huntington Disease (HD), SCA31, 36 and even the whole exome sequencing (WES) were further performed. We investigated the genetic results and clinical characteristics retrospectively. Results Among these 190 index cases, 177(93.16%) were identified SCA dynamic mutations. SCA3 was the commonest, accounting for 70.06%, followed by SCA1 (9.6%), 2 (9.05%), 12 (3.39%), 6 (2.26%), DRPLA (2.26%), 7(1.13%), 8 (1.13%) and 17(0.56%). One patient carried a compound dynamic mutation of SCA6 and SCA17 (SCA6/17). No SCA10 or SCA36 was found. Among the remaining 13 patients, three were diagnosed with HD (1.58%) and one with Episodic Ataxia 2 (EA2). WES did reveal several variants with uncertain significance (VUS) in the remaining nine patients, but failed to detect causative mutations. Conclusion We illustrated the approach and challenge of genetic testing in Chinese ADCA patients. Dynamic mutations of SCAs should be screened firstly. When the results were negative, dynamic mutation of HTT would better be screened consequently. In early-onset ADCA patients, WES might be effective to identify causative mutations, but in adult-onset cases, WES might be less effective.
Primary needle-knife fistulotomy for preventing post-endoscopic retrograde cholangiopancreatography pancreatitis: importance of the endoscopist’s expertise level
Authors: Han Sung Yong , Baek Dong Hoon , Kim Dong Uk , Park Chang Joon , Han In Sub , Kim Gwang Ha , Song Geun Am
Published: February 2020

Background Needle-knife fistulotomy (NKF) is used as a rescue technique for difficult cannulation. However, the data are limited regarding the use of needle-knife fistulotomy for primary biliary cannulation, especially when performed by beginners. To assess the effectiveness and safety of primary NKF for biliary cannulation, and the role of the endoscopist’s expertise level (beginner vs. expert). Methods We retrospectively evaluated the records of 613 patients with naïve papilla and no history of pancreatitis, who underwent bile duct cannulation at a tertiary referral center. The patients were categorized according to the endoscopist’s expertise level and the technique used for bile duct cannulation. We assessed the rates of successful cannulation and adverse events. Results The baseline characteristics did not differ between the pre-defined groups. The incidence rate of post-endoscopic retrograde cholangiopancreatography (ERCP) pancreatitis (PEP) was significantly affected by the endoscopist’s expertise level in patients who received conventional cannulation with sphincterotomy (11.3% vs. 5.7% for beginner vs. expert, P =0.033), but not in those who received NKF. In the multivariable analysis, a lower expertise level of the biliary endoscopist and longer cannulation time were significant risk factor of post-endoscopic retrograde cholangiopancreatography pancreatitis ( P =0.030, P =0.005) in patients who received conventional cannulation with sphincterotomy but not in those who received NKF. Conclusion Compared to conventional cannulation with sphincterotomy, primary NKF carries a lower risk of PEP regardless of the endoscopist’s level of expertise. Primary NKF may be effective and safe in achieving ductal access in patients with naïve papilla.
Clinical Outcomes of Preimplantation Genetic Testing (PGT) Application in Couples with Chromosomal Inversion, a Study in the Chinese Han Population
Authors: Shao Yuhan , Li Jing , Lu Juanjuan , Li Hongchang , Zhu Yueting , Jiang Wenjie , Yan Junhao
Published: February 2020

Background Chromosomal inversion was considered to have adverse effects on pregnancy outcomes through abnormal gametogenesis. The purpose of this retrospective study was to investigate whether preimplantation genetic testing (PGT) improves pregnancy outcomes for couples with chromosomal inversion. Methods A total of 188 cycles from 165 couples with one chromosomal inversion carrier were divided into two groups: PGT (136 cycles, 125 couples) and non-PGT (52 cycles, 50 couples). Biochemical pregnancy, clinical pregnancy, ongoing pregnancy, miscarriage and live birth rates of their first transfer cycles, as well as cumulative live birth rates of each cycle and euploidy rates, were analyzed. Results There were no statistically significant differences in the pregnancy outcomes between the two groups. The euploidy rate of pericentric inversion carriers was not higher than that of paracentric inversion carriers in PGT group (60.71% vs 50.54%, P=0.073). Similarly, the euploid rate of male carriers was not higher than that of female carriers (61.2% vs 56.1%, P=0.256). Conclusions Due to limitation of retrospective study and small sample size, our current data showed that PGT cannot provide prominent benefits for inversion carriers in the Chinese Han population. Further prospective randomized controlled trials are needed to evaluate the effects of PGT.