bluuebunny/crossref_metadata_2025 huggingface dataset:
Centrifugal Compressor Stall Control by the Application of Engineered Surface Roughness on Diffuser Shroud Using Numerical Simulations
Authors: Khan Amjid , Irfan Muhammad , Niazi Usama Muhammad , Shah Imran , Legutko Stanislaw , Rahman Saifur , Alwadie Abdullah Saeed , Jalalah Mohammed , Glowacz Adam , Khan Mohammad Kamal Asif
Published: April 2021

Downsizing in engine size is pushing the automotive industry to operate compressors at low mass flow rate. However, the operation of turbocharger centrifugal compressor at low mass flow rate leads to fluid flow instabilities such as stall. To reduce flow instability, surface roughness is employed as a passive flow control method. This paper evaluates the effect of surface roughness on a turbocharger centrifugal compressor performance. A realistic validation of SRV2-O compressor stage designed and developed by German Aerospace Center (DLR) is achieved from comparison with the experimental data. In the first part, numerical simulations have been performed from stall to choke to study the overall performance variation at design conditions: 2.55 kg/s mass flow rate and rotational speed of 50,000 rpm. In second part, surface roughness of magnitude range 0–200 μm has been applied on the diffuser shroud to control flow instability. It was found that completely rough regime showed effective quantitative results in controlling stall phenomena, which results in increases of operating range from 16% to 18% and stall margin from 5.62% to 7.98%. Surface roughness as a passive flow control method to reduce flow instability in the diffuser section is the novelty of this research. Keeping in view the effects of surface roughness, it will help the turbocharger manufacturers to reduce the flow instabilities in the compressor with ease and improve the overall performance.
Petrographic and Geotechnical Characteristics of Carbonate Aggregates from Poland and Their Correlation with the Design of Road Surface Structures
Authors: Trzciński Jerzy , Wójcik Emilia , Marszałek Mateusz , Łukaszewski Paweł , Krajewski Marek , Styk Stanisław
Published: April 2021

The paper presents the basic problem related with practical application of carbonate rocks in construction: are carbonate aggregates produced from such rocks favorable for building engineering, particularly for road design and construction? To resolve this problem, (1) the geological-engineering properties of aggregates are presented, (2) the correlation between petrographic and engineering parameters is shown, and (3) a strict correlation between the geological-engineering properties and the freezing-thawing and crushing resistance is recognized. This knowledge has allowed to assess the usefulness of asphalt concrete (AC) made from dolomite and limestone aggregates in the design and construction of road surface structures. The petrography was characterized using optical microscopy and scanning electron microscopy (SEM) coupled with energy-dispersive X-ray spectroscope (EDS). Engineering properties were determined in accordance with European and Polish norms and guidelines. Statistical and design calculations were performed using dedicated software. The petrographic properties, and selected physical and mechanical parameters of the aggregates, were tested to show their influence on the freezing–thawing and crushing resistance. Strong functional relationships between the water adsorption, and the freezing–thawing and crushing resistance have been observed. Aggregate strength decreased after saturation with increasing concentrations of salt solutions. Calculations of AC fatigue durability and deformation allow for reducing the thickness of the road surface structure by about 20% in comparison to normative solutions. This conclusion has impact on the economy of road design and construction, and allows for a rational utilization of rock resources, which contributes to sustainable development of the construction industry.
Cold In-Place Recycling Asphalt Mixtures: Laboratory Performance and Preliminary M-E Design Analysis
Authors: Jin Dongzhao , Ge Dongdong , Chen Siyu , Che Tiankai , Liu Hongfu , Malburg Lance , You Zhanping
Published: April 2021

Cold in-place recycling (CIR) asphalt mixtures are an attractive eco-friendly method for rehabilitating asphalt pavement. However, the on-site CIR asphalt mixture generally has a high air void because of the moisture content during construction, and the moisture susceptibility is vital for estimating the road service life. Therefore, the main purpose of this research is to characterize the effect of moisture on the high-temperature and low-temperature performance of a CIR asphalt mixture to predict CIR pavement distress based on a mechanistic–empirical (M-E) pavement design. Moisture conditioning was simulated by the moisture-induced stress tester (MIST). The moisture susceptibility performance of the CIR asphalt mixture (pre-mist and post-mist) was estimated by a dynamic modulus test and a disk-shaped compact tension (DCT) test. In addition, the standard solvent extraction test was used to obtain the reclaimed asphalt pavement (RAP) and CIR asphalt. Asphalt binder performance, including higher temperature and medium temperature performance, was evaluated by dynamic shear rheometer (DSR) equipment and low-temperature properties were estimated by the asphalt binder cracking device (ABCD). Then the predicted pavement distresses were estimated based on the pavement M-E design method. The experimental results revealed that (1) DCT and dynamic modulus tests are sensitive to moisture conditioning. The dynamic modulus decreased by 13% to 43% at various temperatures and frequencies, and the low-temperature cracking energy decreased by 20%. (2) RAP asphalt incorporated with asphalt emulsion decreased the high-temperature rutting resistance but improved the low-temperature anti-cracking and the fatigue life. The M-E design results showed that the RAP incorporated with asphalt emulsion reduced the international roughness index (IRI) and AC bottom-up fatigue predictions, while increasing the total rutting and AC rutting predictions. The moisture damage in the CIR pavement layer also did not significantly affect the predicted distress with low traffic volume. In summary, the implementation of CIR technology in the project improved low-temperature cracking and fatigue performance in the asphalt pavement. Meanwhile, the moisture damage of the CIR asphalt mixture accelerated high-temperature rutting and low-temperature cracking, but it may be acceptable when used for low-volume roads.
The Emergence of Sequential Buckling in Reconfigurable Hexagonal Networks Embedded into Soft Matrix
Authors: Galich Pavel I. , Sharipova Aliya , Slesarenko Slava
Published: April 2021

The extreme and unconventional properties of mechanical metamaterials originate in their sophisticated internal architectures. Traditionally, the architecture of mechanical metamaterials is decided on in the design stage and cannot be altered after fabrication. However, the phenomenon of elastic instability, usually accompanied by a reconfiguration in periodic lattices, can be harnessed to alter their mechanical properties. Here, we study the behavior of mechanical metamaterials consisting of hexagonal networks embedded into a soft matrix. Using finite element analysis, we reveal that under specific conditions, such metamaterials can undergo sequential buckling at two different strain levels. While the first reconfiguration keeps the periodicity of the metamaterial intact, the secondary buckling is accompanied by the change in the global periodicity and formation of a new periodic unit cell. We reveal that the critical strains for the first and the second buckling depend on the metamaterial geometry and the ratio between elastic moduli. Moreover, we demonstrate that the buckling behavior can be further controlled by the placement of the rigid circular inclusions in the rotation centers of order 6. The observed sequential buckling in bulk metamaterials can provide additional routes to program their mechanical behavior and control the propagation of elastic waves.
High Electrochemical Performance Silicon Thin-Film Free-Standing Electrodes Based on Buckypaper for Flexible Lithium-Ion Batteries
Authors: Nyamaa Oyunbayar , Seo Duck-Hyeon , Lee Jun-Seok , Jeong Hyo-Min , Huh Sun-Chul , Yang Jeong-Hyeon , Dolgor Erdenechimeg , Noh Jung-Pil
Published: April 2021

Recently, applications for lithium-ion batteries (LIBs) have expanded to include electric vehicles and electric energy storage systems, extending beyond power sources for portable electronic devices. The power sources of these flexible electronic devices require the creation of thin, light, and flexible power supply devices such as flexile electrolytes/insulators, electrode materials, current collectors, and batteries that play an important role in packaging. Demand will require the progress of modern electrode materials with high capacity, rate capability, cycle stability, electrical conductivity, and mechanical flexibility for the time to come. The integration of high electrical conductivity and flexible buckypaper (oxidized Multi-walled carbon nanotubes (MWCNTs) film) and high theoretical capacity silicon materials are effective for obtaining superior high-energy-density and flexible electrode materials. Therefore, this study focuses on improving the high-capacity, capability-cycling stability of the thin-film Si buckypaper free-standing electrodes for lightweight and flexible energy-supply devices. First, buckypaper (oxidized MWCNTs) was prepared by assembling a free stand-alone electrode, and electrical conductivity tests confirmed that the buckypaper has sufficient electrical conductivity (10−4(S m−1) in LIBs) to operate simultaneously with a current collector. Subsequently, silicon was deposited on the buckypaper via magnetron sputtering. Next, the thin-film Si buckypaper freestanding electrodes were heat-treated at 600 °C in a vacuum, which improved their electrochemical performance significantly. Electrochemical results demonstrated that the electrode capacity can be increased by 27/26 and 95/93 μAh in unheated and heated buckypaper current collectors, respectively. The measured discharge/charge capacities of the USi_HBP electrode were 108/106 μAh after 100 cycles, corresponding to a Coulombic efficiency of 98.1%, whereas the HSi_HBP electrode indicated a discharge/charge capacity of 193/192 μAh at the 100th cycle, corresponding to a capacity retention of 99.5%. In particular, the HSi_HBP electrode can decrease the capacity by less than 1.5% compared with the value of the first cycle after 100 cycles, demonstrating excellent electrochemical stability.
Characterization of Charge States in Conducting Organic Nanoparticles by X-ray Photoemission Spectroscopy
Authors: Fraxedas Jordi , Vollmer Antje , Koch Norbert , de Caro Dominique , Jacob Kane , Faulmann Christophe , Valade Lydie
Published: April 2021

The metallic and semiconducting character of a large family of organic materials based on the electron donor molecule tetrathiafulvalene (TTF) is rooted in the partial oxidation (charge transfer or mixed valency) of TTF derivatives leading to partially filled molecular orbital-based electronic bands. The intrinsic structure of such complexes, with segregated donor and acceptor molecular chains or planes, leads to anisotropic electronic properties (quasi one-dimensional or two-dimensional) and morphology (needle-like or platelet-like crystals). Recently, such materials have been synthesized as nanoparticles by intentionally frustrating the intrinsic anisotropic growth. X-ray photoemission spectroscopy (XPS) has emerged as a valuable technique to characterize the transfer of charge due to its ability to discriminate the different chemical environments or electronic configurations manifested by chemical shifts of core level lines in high-resolution spectra. Since the photoemission process is inherently fast (well below the femtosecond time scale), dynamic processes can be efficiently explored. We determine here the fingerprint of partial oxidation on the photoemission lines of nanoparticles of selected TTF-based conductors.
A Synergy of Innovative Technologies towards Implementing an Autonomous DIY Electric Vehicle for Harvester-Assisting Purposes
Authors: Loukatos Dimitrios , Petrongonas Evangelos , Manes Kostas , Kyrtopoulos Ioannis-Vasileios , Dimou Vasileios , Arvanitis Konstantinos G.
Published: April 2021

The boom in the electronics industry has made a variety of credit card-sized computer systems and plenty of accompanying sensing and acting elements widely available, at continuously diminishing cost and size levels. The benefits of this situation for agriculture are not left unexploited and thus, more accurate, efficient and environmentally-friendly systems are making the scene. In this context, there is an increasing interest in affordable, small-scale agricultural robots. A key factor for success is the balanced selection of innovative hardware and software components, among the plethora being available. This work describes exactly the steps for designing, implementing and testing a small autonomous electric vehicle, able to follow the farmer during the harvesting activities and to carry the fruits/vegetables from the plant area to the truck location. Quite inexpensive GPS and IMU units, assisted by hardware-accelerated machine vision, speech recognition and networking techniques can assure the fluent operation of a prototype vehicle exhibiting elementary automatic control functionality. The whole approach also highlights the challenges for achieving a truly working solution and provides directions for future exploitation and improvements.
Impact of Regional Difference in Recovery Rate on the Total Population of Infected for a Diffusive SIS Model
Authors: Inoue Jumpei , Kuto Kousuke
Published: April 2021

This paper is concerned with an SIS epidemic reaction-diffusion model. The purpose of this paper is to derive some effects of the spatial heterogeneity of the recovery rate on the total population of infected and the reproduction number. The proof is based on an application of our previous result on the unboundedness of the ratio of the species to the resource for a diffusive logistic equation. Our pure mathematical result can be epidemically interpreted as that a regional difference in the recovery rate can make the infected population grow in the case when the reproduction number is slightly larger than one.
Control Method of Flexible Manipulator Servo System Based on a Combination of RBF Neural Network and Pole Placement Strategy
Authors: Shang Dongyang , Li Xiaopeng , Yin Meng , Li Fanjie
Published: April 2021

Gravity and flexibility will cause fluctuations of the rotation angle in the servo system for flexible manipulators. The fluctuation will seriously affect the motion accuracy of end-effectors. Therefore, this paper adopts a control method combining the RBF (Radial Basis Function) neural network and pole placement strategy to suppress the rotation angle fluctuations. The RBF neural network is used to identify uncertain items caused by the manipulator’s flexibility and the time-varying characteristics of dynamic parameters. Besides, the pole placement strategy is used to optimize the PD (Proportional Differential) controller’s parameters to improve the response speed and stability. Firstly, a dynamic model of flexible manipulators considering gravity is established based on the assumed mode method and Lagrange’s principle. Then, the system’s control characteristics are analyzed, and the pole placement strategy optimizes the parameters of the PD controllers. Next, the control method based on the RBF neural network is proposed, and the Lyapunov stability theory demonstrates stability. Finally, numerical analysis and control experiments prove the effectiveness of the control method proposed in this paper. The means and standard deviations of rotation angle error are reduced by the control method. The results show that the control method can effectively reduce the rotation angle error and improve motion accuracy.
An Approximation of Solutions for the Problem with Quasistatic Contact in the Case of Dry Friction
Authors: Pop Nicolae , Ungureanu Miorita , Pop Adrian I.
Published: April 2021

In this paper, we discuss the question of finding an optimal control for the solutions of the problem with dry friction quasistatic contact, in the case that the friction law is modeled by a nonlocal version of Coulomb’s law. In order to get the necessary optimality conditions, we use some regularization techniques, and this leads us to a problem of control for an inequality of the variational type. The optimal control problem consists, in our case, of minimizing a sequence of optimal control problems, where the control variable is given by a Neumann-type boundary condition. The state system is represented by a limit of a sequence, whose terms are obtained from the discretization, in time with finite difference and space with the finite element method of a regularized quasistatic contact problem with Coulomb friction. The purpose of this optimal control problem is that the traction force (the control variable) acting on one side of the boundary (the Neumann boundary condition) of the elastic body produces a displacement field (the state system solution) close enough to the imposed displacement field, and the traction force from the boundary remains small enough.
The Role of Prevention in Reducing the Economic Impact of ME/CFS in Europe: A Report from the Socioeconomics Working Group of the European Network on ME/CFS (EUROMENE)
Authors: Pheby Derek F. H. , Araja Diana , Berkis Uldis , Brenna Elenka , Cullinan John , de Korwin Jean-Dominique , Gitto Lara , Hughes Dyfrig A. , Hunter Rachael M. , Trepel Dominic , Wang-Steverding Xia
Published: April 2021

This report addresses the extent to which there may be scope for preventive programmes for Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS), and, if so, what economic benefits may accrue from the implementation of such programmes. We consider the economic case for prevention programmes, whether there is scope for preventive programmes for ME/CFS, and what are the health and economic benefits to be derived from the implementation of such programmes. We conclude that there is little scope for primary prevention programmes, given that ME/CFS is attributable to a combination of host and environmental risk factors, with host factors appearing to be most prominent, and that there are few identified modifiable risk factors that could be the focus of such programmes. The exception is in the use of agricultural chemicals, particularly organophosphates, where there is scope for intervention, and where Europe-wide programmes of health education to encourage safe use would be beneficial. There is a need for more research on risk factors for ME/CFS to establish a basis for the development of primary prevention programmes, particularly in respect of occupational risk factors. Secondary prevention offers the greatest scope for intervention, to minimise diagnostic delays associated with prolonged illness, increased severity, and increased costs.
The p38/MK2 Axis in Monocytes of Fibromyalgia Syndrome Patients: An Explorative Study
Authors: Nugraha Boya , Scheibe Renate , Korallus Christoph , Gaestel Matthias , Gutenbrunner Christoph
Published: April 2021

Background and Objectives: The aetiology and pathomechanism of fibromyalgia syndrome 12 (FMS) as one of chronic pain syndromes still need to be further elucidated. Mitogen-activated protein kinase (MAPK) pathway has been proposed as a novel approach in pain management. Since the major symptom of fibromyalgia syndrome (FMS) patients is pain, it became of interest whether MAPK pathways, such as the stress-activated p38 MAPK/MK2 axis, are activated in FMS patients. Therefore, this study aimed at determining p38 MAPK/MK2 in FMS patients. Materials and Methods: Phosphorylation of MAPK-activated protein kinases 2 (MK2), a direct target of p38 MAPK, was measured in monocytes of FMS and healthy controls (HCs) to monitor the activity of this pathway. Results: The mean level of phosphorylated MK2 was fivefold higher in FMS patients as compared to HCs (p < 0.001). Subgroup analysis revealed that antidepressants did not influence the activity of MK2 in FMS patients. Conclusions: This result indicates that the p38/MK2 pathway could be involved in the pathomechanism of FMS, could act as a clinical marker for FMS, and could be a possible target for pain management in FMS patients.
Modeling of Inclusion Capture in a Steel Slab Caster with Vertical Section and Bending
Authors: Cho Seong-Mook , Thomas Brian G. , Hwang Jong-Yeon , Bang Jong-Geun , Bae Il-Sin
Published: April 2021

Particles in molten steel, including argon-gas bubbles, slag droplets, and non-metallic inclusions, are removed into the surface-slag layer or captured by the solidifying steel-shell during continuous steel casting. Captured particles often become serious defects in the final steel product, so understanding particle-capture mechanisms is important for steel quality. Slab casters often have a straight mold and upper-strand prior to a curved lower-strand. The present work investigates particle capture in such a caster using computational modeling with a standard k-ε model for molten-steel flow, a discrete phase model for inclusion transport, and an advanced capture criterion for inclusion entrapment and engulfment into the steel shell. A new postprocessing methodology is presented and applied to predict inclusion-capture rates in commercial cast product. The locations and size distributions of particles captured into the shell, and actual capture rates are quantified. The model predictions are validated with ultrasonic-test plant measurements of the locations of large particles captured in a steel slab. The results reveal how large-inclusion capture accumulates in the beginning of the curved strand, leading to a capture band in the slab inside radius. Finally, the capture fractions and locations due to all capture mechanisms are compared for different inclusion sizes, and the implications are discussed.
Nonisothermal Kinetic Degradation of Hybrid CNT/Alumina Epoxy Nanocomposites
Authors: Kudus Muhammad Helmi Abdul , Zakaria Muhammad Razlan , Omar Mohd Firdaus , Hafi Othman Muhammad Bisyrul , Md. Akil Hazizan , Nabiałek Marcin , Jeż Bartłomiej , Abdullah Mohd Mustafa Al Bakri
Published: April 2021

Due to the synergistic effect that occurs between CNTs and alumina, CNT/alumina hybrid-filled epoxy nanocomposites show significant enhancements in tensile properties, flexural properties, and thermal conductivity. This study is an extension of previously reported investigations into CNT/alumina epoxy nanocomposites. A series of epoxy composites with different CNT/alumina loadings were investigated with regard to their thermal-degradation kinetics and lifetime prediction. The thermal-degradation parameters were acquired via thermogravimetric analysis (TGA) in a nitrogen atmosphere. The degradation activation energy was determined using the Flynn–Wall–Ozawa (F-W-O) method for the chosen apparent activation energy. The Ea showed significant differences at α > 0.6, which indicate the role played by the CNT/alumina hybrid filler loading in the degradation behavior. From the calculations, the lifetime prediction at 5% mass loss decreased with an increase in the temperature service of nitrogen. The increase in the CNT/alumina hybrid loading revealed its contribution towards thermal degradation and stability. On average, a higher Ea was attributed to greater loadings of the CNT/alumina hybrid in the composites.
Improved Bone Quality and Bone Healing of Dystrophic Mice by Parabiosis
Authors: Li Hongshuai , Lu Aiping , Gao Xueqin , Tang Ying , Ravuri Sudheer , Wang Bing , Huard Johnny
Published: April 2021

Duchenne muscular dystrophy (DMD) is a degenerative muscle disorder characterized by a lack of dystrophin expression in the sarcolemma of muscle fibers. DMD patients acquire bone abnormalities including osteopenia, fragility fractures, and scoliosis indicating a deficiency in skeletal homeostasis. The dKO (dystrophin/Utrophin double knockout) is a more severe mouse model of DMD than the mdx mouse (dystrophin deficient), and display numerous clinically-relevant manifestations, including a spectrum of degenerative changes outside skeletal muscle including bone, articular cartilage, and intervertebral discs. To examine the influence of systemic factors on the bone abnormalities and healing in DMD, parabiotic pairing between dKO mice and mdx mice was established. Notably, heterochronic parabiosis with young mdx mice significantly increased bone mass and improved bone micro-structure in old dKO-hetero mice, which showed progressive bone deterioration. Furthermore, heterochronic parabiosis with WT C56/10J mice significantly improved tibia bone defect healing in dKO-homo mice. These results suggest that systemic blood-borne factor(s) and/or progenitors from WT and young mdx mice can influence the bone deficiencies in dKO mice. Understanding these circulating factors or progenitor cells that are responsible to alleviate the bone abnormalities in dKO mice after heterochronic parabiosis might be useful for the management of poor bone health in DMD.
Brazilian Coffee Production and the Future Microbiome and Mycotoxin Profile Considering the Climate Change Scenario
Authors: dos Santos Deiziane Gomes , Coelho Caroline Corrêa de Souza , Ferreira Anna Beatriz Robottom , Freitas-Silva Otniel
Published: April 2021

Brazil holds a series of favorable climatic conditions for agricultural production including the hours and intensity of sunlight, the availability of agricultural land and water resources, as well as diverse climates, soils and biomes. Amidst such diversity, Brazilian coffee producers have obtained various standards of qualities and aromas, between the arabica and robusta species, which each present a wide variety of lineages. However, temperatures in coffee producing municipalities in Brazil have increased by about 0.25 °C per decade and annual precipitation has decreased. Therefore, the agricultural sector may face serious challenges in the upcoming decades due to crop sensitivity to water shortages and thermal stress. Furthermore, higher temperatures may reduce the quality of the culture and increase pressure from pests and diseases, reducing worldwide agricultural production. The impacts of climate change directly affect the coffee microbiota. Within the climate change scenario, aflatoxins, which are more toxic than OTA, may become dominant, promoting greater food insecurity surrounding coffee production. Thus, closer attention on the part of authorities is fundamental to stimulate replacement of areas that are apt for coffee production, in line with changes in climate zoning, in order to avoid scarcity of coffee in the world market.
Current Status of Putative Animal Sources of SARS-CoV-2 Infection in Humans: Wildlife, Domestic Animals and Pets
Authors: Maurin Max , Fenollar Florence , Mediannikov Oleg , Davoust Bernard , Devaux Christian , Raoult Didier
Published: April 2021

SARS-CoV-2 is currently considered to have emerged from a bat coronavirus reservoir. However, the real natural cycle of this virus remains to be elucidated. Moreover, the COVID-19 pandemic has led to novel opportunities for SARS-CoV-2 transmission between humans and susceptible animal species. In silico and in vitro evaluation of the interactions between the SARS-CoV-2 spike protein and eucaryotic angiotensin-converting enzyme 2 (ACE2) receptor have tentatively predicted susceptibility to SARS-CoV-2 infection of several animal species. Although useful, these data do not always correlate with in vivo data obtained in experimental models or during natural infections. Other host biological properties may intervene such as the body temperature, level of receptor expression, co-receptor, restriction factors, and genetic background. The spread of SARS-CoV-2 also depends on the extent and duration of viral shedding in the infected host as well as population density and behaviour (group living and grooming). Overall, current data indicate that the most at-risk interactions between humans and animals for COVID-19 infection are those involving certain mustelids (such as minks and ferrets), rodents (such as hamsters), lagomorphs (especially rabbits), and felines (including cats). Therefore, special attention should be paid to the risk of SARS-CoV-2 infection associated with pets.
Escherichia coli Affects Expression of Circadian Clock Genes in Human Hepatoma Cells
Authors: Kovač Urša , Žužek Zala , Raspor Dall’Olio Lucija , Pohar Katka , Ihan Alojz , Moškon Miha , Rozman Damjana , Starčič Erjavec Marjanca
Published: April 2021

Recent research has indicated that dysbiosis of the gut microbiota can lead to an altered circadian clock of the mammalian host. Herein we developed an original system that allows real-time circadian studies of human HepG2 hepatoma cells co-cultured with bacteria. The HepG2 cells with stably integrated firefly luciferase reporter under the control of PERIOD2 promoter were co-cultured with E. coli strains isolated from human fecal samples from healthy individuals. The two E. coli strains differ in the phylogenetic group and the number of ExPEC virulence-associated genes: BJ17 has only two, and BJ23 has 15 of 23 tested. In the first 24 h, the E. coli BJ17 affected the HepG2 circadian clock more than BJ23. Cosinor analysis shows a statistically significant change in the amplitude of PER1 and 2 and the phase advance of PER3. A high percentage of necrotic and apoptotic cells occurred at 72 h, while a correlation between the number of ExPEC genes and the influence on the HepG2 core clock gene expression was observed. Our study reveals that the E. coli genetic background is important for the effect on the mammalian circadian clock genes, indicating possible future use of probiotic E. coli strains to influence the host circadian clock.
Functional Expression, Purification and Identification of Interaction Partners of PACRG
Authors: Liu Tiankai , Zhao Haizhou , Jian Shaofen , Gong Shu , Li Sainan , Ma Yanhong , Chen Jun , Liu Wenhua
Published: April 2021

PACRG (Parkin co-regulated gene) shares a bi-directional promoter with the Parkinson’s disease-associated gene Parkin, but the physiological roles of PACRG have not yet been fully elucidated. Recombinant expression methods are indispensable for protein structural and functional studies. In this study, the coding region of PACRG was cloned to a conventional vector pQE80L, as well as two cold-shock vectors pCold II and pCold-GST, respectively. The constructs were transformed into Escherichia coli (DE3), and the target proteins were overexpressed. The results showed that the cold-shock vectors are more suitable for PACRG expression. The soluble recombinant proteins were purified with Ni2+ chelating column, glutathione S-transferase (GST) affinity chromatography and gel filtration. His6 pull down assay and LC-MS/MS were carried out for identification of PACRG-binding proteins in HEK293T cell lysates, and a total number of 74 proteins were identified as potential interaction partners of PACRG. GO (Gene ontology) enrichment analysis (FunRich) of the 74 proteins revealed multiple molecular functions and biological processes. The highest proportion of the 74 proteins functioned as transcription regulator and transcription factor activity, suggesting that PACRG may play important roles in regulation of gene transcription.
Protective Effects of Cinnamaldehyde on the Inflammatory Response, Oxidative Stress, and Apoptosis in Liver of Salmonella typhimurium-Challenged Mice
Authors: Wang Renjie , Li Senlin , Jia Hai , Si Xuemeng , Lei Yan , Lyu Jirong , Dai Zhaolai , Wu Zhenlong
Published: April 2021

Salmonella typhimurium infection is associated with gastrointestinal disorder and cellular injury in the liver of both humans and animals. Cinnamaldehyde, the main component of essential oil from cinnamon, has been reported to have anti-inflammatory, anti-oxidative, and anti-apoptotic effects. However, it remains unknown whether cinnamaldehyde can alleviate Salmonella typhimurium infection-induced liver injury in mice. In the present study, we found that cinnamaldehyde attenuated Salmonella typhimurium-induced body weight loss, the increase of organ (liver and spleen) indexes, hepatocyte apoptosis, and the mortality rate in mice. Further study showed that cinnamaldehyde significantly alleviated Salmonella typhimurium-induced liver injury as shown by activities of alanine transaminase, aspartate transaminase, and myeloperoxidase, as well as malondialdehyde. The increased mRNA level of pro-inflammatory cytokines (IL-1β, IL-6, TNF-α, and IFN-γ) and chemokines (CCL2 and CCL3) induced by Salmonella typhimurium were significantly abolished by cinnamaldehyde supplementation. These alterations were associated with a regulatory effect of cinnamaldehyde on TLR2, TLR4, and MyD88. 16S rDNA sequence analysis showed that Salmonella typhimurium infection led to upregulation of the abundances of genera Akkermansia, Bacteroides, Alistipes, Muribaculum, and Prevotellaceae UCG-001, and downregulation of the abundances of genera Lactobacillus, Enterorhabdus, and Eggerthellaceae (unclassified). These alterations were reversed by cinnamaldehyde supplementation. In conclusion, cinnamaldehyde attenuated the inflammatory response, oxidative stress, and apoptosis in the liver of Salmonella typhimurium-infected mice. Supplementation of cinnamaldehyde might be a preventive strategy to alleviate liver injury caused by Salmonella typhimurium infection in humans and animals.
Disposable Electrochemical Sensor for Food Colorants Detection by Reduced Graphene Oxide and Methionine Film Modified Screen Printed Carbon Electrode
Authors: Akkapinyo Chutimon , Subannajui Kittitat , Poo-arporn Yingyot , Poo-arporn Rungtiva P.
Published: April 2021

A facile synthesis of reduced graphene oxide (rGO) and methionine film modified screen printed carbon electrode (rGO-methionine/SPCE) was proposed as a disposable sensor for determination of food colorants including amaranth, tartrazine, sunset yellow, and carminic acid. The fabrication process can be achieved in only 2 steps including drop-casting of rGO and electropolymerization of poly(L-methionine) film on SPCE. Surface morphology of modified electrode was studied by scanning electron microscopy (SEM). This work showed a successfully developed novel disposable sensor for detection of all 4 dyes as food colorants. The electrochemical behavior of all 4 food colorants were investigated on modified electrodes. The rGO-methionine/SPCE significantly enhanced catalytic activity of all 4 dyes. The pH value and accumulation time were optimized to obtain optimal condition of each colorant. Differential pulse voltammetry (DPV) was used for determination, and two linear detection ranges were observed for each dye. Linear detection ranges were found from 1 to 10 and 10 to 100 µM for amaranth, 1 to 10 and 10 to 85 µM for tartrazine, 1 to 10 and 10 to 50 µM for sunset yellow, and 1 to 20 and 20 to 60 µM for carminic acid. The limit of detection (LOD) was calculated at 57, 41, 48, and 36 nM for amaranth, tartrazine, sunset yellow, and carminic acid, respectively. In addition, the modified sensor also demonstrated high tolerance to interference substances, good repeatability, and high performance for real sample analysis.
A Zinc-Mediated Deprotective Annulation Approach to New Polycyclic Heterocycles
Authors: Veltri Lucia , Amuso Roberta , Petrilli Marzia , Cuocci Corrado , Chiacchio Maria A. , Vitale Paola , Gabriele Bartolo
Published: April 2021

A straightforward approach to new polycyclic heterocycles, 1H-benzo[4,5]imidazo[1,2-c][1,3]oxazin-1-ones, is presented. It is based on the ZnCl2-promoted deprotective 6-endo-dig heterocyclization of N-Boc-2-alkynylbenzimidazoles under mild conditions (CH2Cl2, 40 °C for 3 h). The zinc center plays a dual role, as it promotes Boc deprotection (with formation of the tert-butyl carbocation, which can be trapped by substrates bearing a nucleophilic group) and activates the triple bond toward intramolecular nucleophilic attack by the carbamate group. The structure of representative products has been confirmed by X-ray diffraction analysis.
Curcumin Inhibits Lysophosphatidic Acid Mediated MCP-1 Expression via Blocking ROCK Signalling
Authors: Zhou Ying , Little Peter J. , Xu Suowen , Kamato Danielle
Published: April 2021

Curcumin is a natural compound that has been widely used as a food additive and medicine in Asian countries. Over several decades, diverse biological effects of curcumin have been elucidated, such as anti-inflammatory and anti-oxidative activities. Monocyte chemoattractant protein-1 (MCP-1) is a key inflammatory marker during the development of atherosclerosis, and curcumin blocks MCP-1 expression stimulated by various ligands. Hence, we studied the action of curcumin on lysophosphatidic acid (LPA) mediated MCP-1 expression and explored the specific underlying mechanisms. In human vascular smooth muscle cells, LPA induces Rho-associated protein kinase (ROCK) dependent transforming growth factor receptor (TGFBR1) transactivation, leading to glycosaminoglycan chain elongation. We found that LPA also signals via the TGFBR1 transactivation pathway to regulate MCP-1 expression. Curcumin blocks LPA mediated TGFBR1 transactivation and subsequent MCP-1 expression by blocking the ROCK signalling. In the vasculature, ROCK signalling regulates smooth muscle cell contraction, inflammatory cell recruitment, endothelial dysfunction and vascular remodelling. Therefore, curcumin as a ROCK signalling inhibitor has the potential to prevent atherogenesis via multiple ways.
Piper betle (L): Recent Review of Antibacterial and Antifungal Properties, Safety Profiles, and Commercial Applications
Authors: Nayaka Ni Made Dwi Mara Widyani , Sasadara Maria Malida Vernandes , Sanjaya Dwi Arymbhi , Yuda Putu Era Sandhi Kusuma , Dewi Ni Luh Kade Arman Anita , Cahyaningsih Erna , Hartati Rika
Published: April 2021

Piper betle (L) is a popular medicinal plant in Asia. Plant leaves have been used as a traditional medicine to treat various health conditions. It is highly abundant and inexpensive, therefore promoting further research and industrialization development, including in the food and pharmaceutical industries. Articles published from 2010 to 2020 were reviewed in detail to show recent updates on the antibacterial and antifungal properties of betel leaves. This current review showed that betel leaves extract, essential oil, preparations, and isolates could inhibit microbial growth and kill various Gram-negative and Gram-positive bacteria as well as fungal species, including those that are multidrug-resistant and cause serious infectious diseases. P. betle leaves displayed high efficiency on Gram-negative bacteria such as Escherichia coli and Pseudomonas aeruginosa, Gram-positive bacteria such as Staphylococcus aureus, and Candida albicans. The ratio of MBC/MIC indicated bactericidal and bacteriostatic effects of P. betle leaves, while MFC/MIC values showed fungicidal and fungistatic effects. This review also provides a list of phytochemical compounds in betel leaves extracts and essential oils, safety profiles, and value-added products of betel leaves. Some studies also showed that the combination of betel leaves extract and essential oil with antibiotics (streptomycin, chloramphenicol and gentamicin) could provide potentiating antibacterial properties. Moreover, this review delivers a scientific resume for researchers in respected areas and manufacturers who want to develop betel leaves-based products.
Optical Characterization of Ciprofloxacin Photolytic Degradation by UV-Pulsed Laser Radiation
Authors: Tozar Tatiana , Boni Mihai , Staicu Angela , Pascu Mihail Lucian
Published: April 2021

Ciprofloxacin is one of the most prescribed antibiotics in treating bacterial infections, becoming an important pollutant of the wastewaters. Moreover, ciprofloxacin is hard to be destroyed by conventional water treatment processes; therefore, efficient treatments to destroy it are needed in water decontamination. This study offers insights into the performance of 266 nm laser beams on the photodegradation of ciprofloxacin. An Nd:YAG laser was used that emitted 266 nm at an energy of 6.5 mJ (power of 65 mW) and ciprofloxacin water solutions were irradiated up to 240 min. The irradiated solutions were investigated by UV-Vis and FTIR absorption spectroscopy, pH assay, and laser-induced fluorescence. An HPTLC densitometer was used to characterize the laser-induced fluorescence and fluorescence lifetime of photodegradation products. The UV-Vis absorption, FTIR, and laser-induced fluorescence spectra showed the degradation of ciprofloxacin. Moreover, HPTLC densitometry offered the fluorescence and fluorescence lifetime of ciprofloxacin and its three photoproducts as well as their relative quantification. From the FTIR spectra, the molecular structure of two out of three photoproducts was proposed. In conclusion, the laser irradiation method provided the efficient photodegradation of ciprofloxacin, whereas the analytical techniques offered the proper means to monitor the process and detect the obtained photoproducts.
Micro Salting-Out Assisted Matrix Solid-Phase Dispersion: A Simple and Fast Sample Preparation Method for the Analysis of Bisphenol Contaminants in Bee Pollen
Authors: Zhang Jianing , Yu Fengjie , Tao Yunmin , Du Chunping , Yang Wenchao , Chen Wenbin , Tu Xijuan
Published: April 2021

In the present work, a novel sample preparation method, micro salting-out assisted matrix solid-phase dispersion (μ-SOA-MSPD), was developed for the determination of bisphenol A (BPA) and bisphenol B (BPB) contaminants in bee pollen. The proposed method was designed to combine two classical sample preparation methodologies, matrix solid-phase dispersion (MSPD) and homogenous liquid-liquid extraction (HLLE), to simplify and speed-up the preparation process. Parameters of μ-SOA-MSPD were systematically investigated, and results indicated the significant effect of salt and ACN-H2O extractant on the signal response of analytes. In addition, excellent clean-up ability in removing matrix components was observed when primary secondary amine (PSA) sorbent was introduced into the blending operation. The developed method was fully validated, and the limits of detection for BPA and BPB were 20 μg/kg and 30 μg/kg, respectively. Average recoveries and precisions were ranged from 83.03% to 94.64% and 1.76% to 5.45%, respectively. This is the first report on the analysis of bisphenol contaminants in bee pollen sample, and also on the combination of MSPD and HLLE. The present method might provide a new strategy for simple and fast sample preparation of solid and semi-solid samples.
Study on Extraction Process of Root of Henry Wood Betony Polysaccharides and Their Antitumor Activity against S180
Authors: Feng Haibo , Tian Lan
Published: April 2021

We optimized the hot water extraction of polysaccharides from the root of Henry wood betony (RHWPs) using a uniform test and explored their anti-tumor activities in vitro and in vivo. The optimal extraction conditions were as follows: 40 min extraction time, liquid/solid ratio 30 mL/g, 100 min soaking time, two extraction cycles, 100% ethanol concentration, and extraction temperature of 80 °C. The molecular weight distribution of RHWPs with MWs was 228,600 g/mol and 5001 g/mol. The IR spectrum further indicated that RHWPs are acidic polysaccharides containing pyranose and furan rings. The main monosaccharides found in RHWPs were mannose, ribose, l-rhamnose monohydrate, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose, and fucose. RHWPs inhibited the proliferation of S180 tumor cells and induced apoptosis in vitro. Oral administration of RHWPs to tumor-bearing mice significantly inhibited the growth of the S180 xenografts, accelerated apoptosis in tumor cells, and expanded the necrotic regions. Furthermore, RHWPs also markedly increased the levels of TNF-α, IFN-γ, and IL-2 in the sera of tumor-bearing mice, and activated immune cells such as lymphocytes, NK cells, and macrophages, thereby inducing tumor cell apoptosis. Taken together, RHWPs are a promising anti-tumor agent that ought to be explored further.
Bioactive Compounds Obtained from Polish “Marynka” Hop Variety Using Efficient Two-Step Supercritical Fluid Extraction and Comparison of Their Antibacterial, Cytotoxic, and Anti-Proliferative Activities In Vitro
Authors: Klimek Katarzyna , Tyśkiewicz Katarzyna , Miazga-Karska Malgorzata , Dębczak Agnieszka , Rój Edward , Ginalska Grazyna
Published: April 2021

Given the health-beneficial properties of compounds from hop, there is still a growing trend towards developing successful extraction methods with the highest yield and also receiving the products with high added value. The aim of this study was to develop efficient extraction method for isolation of bioactive compounds from the Polish “Marynka” hop variety. The modified two-step supercritical fluid extraction allowed to obtain two hop samples, namely crude extract (E1), composed of α-acids, β-acids, and terpene derivatives, as well as pure xanthohumol with higher yield than that of other available methods. The post-extraction residues (R1) were re-extracted in order to obtain extract E2 enriched in xanthohumol. Then, both samples were subjected to investigation of their antibacterial (anti-acne, anti-caries), cytotoxic, and anti-proliferative activities in vitro. It was demonstrated that extract (E1) possessed more beneficial biological properties than xanthohumol. It exhibited not only better antibacterial activity against Gram-positive bacteria strains (MIC, MBC) but also possessed a higher synergistic effect with commercial antibiotics when compared to xanthohumol. Moreover, cell culture experiments revealed that crude extract neither inhibited viability nor divisions of normal skin fibroblasts as strongly as xanthohumol. In turn, calculated selectivity indexes showed that the crude extract had from slightly to significantly better selective anti-proliferative activity towards cancer cells in comparison with xanthohumol.
Synthesis and Single Crystal Structures of N-Substituted Benzamides and Their Chemoselective Selenation/Reduction Derivatives
Authors: Hua Guoxiong , Carpenter-Warren Cameron L. , Cordes David B. , Slawin Alexandra M. Z. , Woollins J. Derek
Published: April 2021

A series of N-aryl-N-(2-oxo-2-arylethyl) benzamides and cinnamides has been prepared. The reaction of the benzamides with Woollins’ reagent, a highly efficient chemoselective selenation/reduction reagent, gave the corresponding N-aryl-N-(arylenethyl) benzoselenoamides in good yields. Five representative single crystal X-ray structures are discussed.