bluuebunny/crossref_metadata_2025 huggingface dataset:
Generation of a Pure Culture of Neuron-like Cells with a Glutamatergic Phenotype from Mouse Astrocytes
Authors: Fernandes Gary Stanley , Singh Rishabh Deo , Kim Kyeong Kyu
Published: April 2022

Astrocyte-to-neuron reprogramming is a promising therapeutic approach for treatment of neurodegenerative diseases. The use of small molecules as an alternative to the virus-mediated ectopic expression of lineage-specific transcription factors negates the tumorigenic risk associated with viral genetic manipulation and uncontrolled differentiation of stem cells. However, because previously developed methods for small-molecule reprogramming of astrocytes to neurons are multistep, complex, and lengthy, their applications in biomedicine, including clinical treatment, are limited. Therefore, our objective in this study was to develop a novel chemical-based approach to the cellular reprogramming of astrocytes into neurons with high efficiency and low complexity. To accomplish that, we used C8-D1a, a mouse astrocyte cell line, to assess the role of small molecules in reprogramming protocols that otherwise suffer from inconsistencies caused by variations in donor of the primary cell. We developed a new protocol by which a chemical mixture formulated with Y26732, DAPT, RepSox, CHIR99021, ruxolitinib, and SAG rapidly and efficiently induced the neural reprogramming of astrocytes in four days, with a conversion efficiency of 82 ± 6%. Upon exposure to the maturation medium, those reprogrammed cells acquired a glutaminergic phenotype over the next eleven days. We also demonstrated the neuronal functionality of the induced cells by confirming KCL-induced calcium flux.
Environmentally Acquired Bacillus and Their Role in C. difficile Colonization Resistance
Authors: Ferreira William T. , Hong Huynh A. , Adams James R. G. , Hess Mateusz , Kotowicz Natalia K. , Tan Sisareuth , Ferrari Enrico , Brisson Alain , Zentek Jurgen , Soloviev Mikhail , Cutting Simon M.
Published: April 2022

Clostridioides difficile is an environmentally acquired, anaerobic, spore-forming bacterium which ordinarily causes disease following antibiotic-mediated dysbiosis of the intestinal microbiota. Although much is understood regarding the life cycle of C. difficile, the fate of C. difficile spores upon ingestion remains unclear, and the underlying factors that predispose an individual to colonization and subsequent development of C. difficile infection (CDI) are not fully understood. Here, we show that Bacillus, a ubiquitous and environmentally acquired, spore-forming bacterium is associated with colonization resistance to C. difficile. Using animal models, we first provide evidence that animals housed under conditions that mimic reduced environmental exposure have an increased susceptibility to CDI, correlating with a loss in Bacillus. Lipopeptide micelles (~10 nm) produced by some Bacilli isolated from the gastro-intestinal (GI)-tract and shown to have potent inhibitory activity to C. difficile have recently been reported. We show here that these micelles, that we refer to as heterogenous lipopeptide lytic micelles (HELMs), act synergistically with components present in the small intestine to augment inhibitory activity against C. difficile. Finally, we show that provision of HELM-producing Bacillus to microbiota-depleted animals suppresses C. difficile colonization thereby demonstrating the significant role played by Bacillus in colonization resistance. In the wider context, our study further demonstrates the importance of environmental microbes on susceptibility to pathogen colonization.
Application of Self-Assembly Nanoparticles Based on DVDMS for Fenton-Like Ion Delivery and Enhanced Sonodynamic Therapy
Authors: Liu Jinqiang , Fu Shiying , Xie Jiaxuan , Zhang Jianzhong , Pan Jintao , Chu Chengchao , Liu Gang , Ju Shenghong
Published: April 2022

Upon harnessing low-intensity ultrasound to activate sonosensitizers, sonodynamic therapy (SDT) induces cancer cell death through the reactive oxygen species (ROS) mediated pathway. Compared with photodynamic therapy (PDT), SDT possesses numerous advantages, including deeper tissue penetration, higher accuracy, fewer side effects, and better patient compliance. Sinoporphyrin sodium (DVDMS), a sonosensitizer approved by the FDA, has drawn abundant attention in clinical research, but there are some deficiencies. In order to further improve the efficiency of DVDMS, many studies have applied self-assembly nanotechnology to modify it. Furthermore, the combined applications of SDT/chemodynamic therapy (CDT) have become a research hotspot in tumor therapy. Therefore, we explored the self-assembly of nanoparticles based on DVDMS and copper to combine SDT and CDT. A cost-effective sonosensitizer was synthesized by dropping CuCl2 into the DVDMS solution with the assistance of PVP. The results revealed that the nanostructures could exert excellent treatment effects on tumor therapy and perform well for PET imaging, indicating the potential for cancer theranostics. In vitro and in vivo experiments showed that the nanoparticles have outstanding biocompatibility, higher ROS production efficiency, and antitumor efficacy. We believe this design can represent a simple approach to combining SDT and CDT with potential applications in clinical treatment and PET imaging.
Nanomechanical Sensors for Gas Detection towards Artificial Olfaction
Authors: Minami Kosuke
Published: April 2022

Humans, as well as other organisms, tend to recognize their surroundings by smells/odors [...]
Changes in the Intranetwork and Internetwork Connectivity of the Default Mode Network and Olfactory Network in Patients with COVID-19 and Olfactory Dysfunction
Authors: Zhang Hui , Chung Tom Wai-Hin , Wong Fergus Kai-Chuen , Hung Ivan Fan-Ngai , Mak Henry Ka-Fung
Published: April 2022

Olfactory dysfunction (OD) is a common symptom in coronavirus disease 2019 (COVID-19) patients. Moreover, many neurological manifestations have been reported in these patients, suggesting central nervous system involvement. The default mode network (DMN) is closely associated with olfactory processing. In this study, we investigated the internetwork and intranetwork connectivity of the DMN and the olfactory network (ON) in 13 healthy controls and 22 patients presenting with COVID-19-related OD using independent component analysis and region of interest functional magnetic resonance imaging (fMRI) analysis. There was a significant correlation between the butanol threshold test (BTT) and the intranetwork connectivity in ON. Meanwhile, the COVID-19 patients with OD showed significantly higher intranetwork connectivity in the DMN, as well as higher internetwork connectivity between ON and DMN. However, no significant difference was found between groups in the intranetwork connectivity within ON. We postulate that higher intranetwork functional connectivities compensate for the deficits in olfactory processing and general well-being in COVID-19 patients. Nevertheless, the compensation process in the ON may not be obvious at this stage. Our results suggest that resting-state fMRI is a potentially valuable tool to evaluate neurosensory dysfunction in COVID-19 patients.
Does Olfactory Dysfunction Correlate with Disease Progression in Parkinson’s Disease? A Systematic Review of the Current Literature
Authors: Ercoli Tommaso , Masala Carla , Cadeddu Gianluca , Mascia Marcello Mario , Orofino Gianni , Gigante Angelo Fabio , Solla Paolo , Defazio Giovanni , Rocchi Lorenzo
Published: April 2022

Background. Loss of olfaction is a well-established early feature of Parkinson’s disease (PD). Although olfactory dysfunction has been widely described as a prodromal feature of PD in the literature, whether it can be considered a biomarker of PD progression is still a matter of debate. Objective. The aim of this work is to define the possible relationship between the progression of olfactory dysfunction and other putative clinical hallmarks of PD over time, through a systematic review of the current literature. Methods. We conducted a systematic review of the literature on PubMed from inception to March 2022. We included only longitudinal studies conducted on patients with diagnosis of idiopathic PD who underwent olfactory function testing at baseline and repeated it at least once during follow-up. Results. Among 5740 records identified through database searching, nine longitudinal studies met full criteria and underwent data extraction. Conclusions. Olfaction seemed to decrease over time, albeit with a degree of fluctuation. Moreover, smell detection ability seems to deteriorate more rapidly in the early phase of disease, indicating a possible association with disease progression. More studies are needed to better understand the role of olfaction as a biomarker of PD progression over time.
Antecedents for Older Adults’ Intention to Use Smart Health Wearable Devices-Technology Anxiety as a Moderator
Authors: Jeng Mei-Yuan , Pai Fan-Yun , Yeh Tsu-Ming
Published: April 2022

The increase in the demands for surveillance of chronic diseases, long-term care, and self-health management has allowed mobile smart health wearable devices to become products with greater business potential in past years. Wearable devices being able to be worn for long periods are the most suitable for 24-h weatherproof monitoring. Nevertheless, most technological products are not developed specifically for older adults. Older adults might be apprehensive and fearful about the use of technological equipment and might appear “technologically anxious”, so it was wondered whether older adults could smoothly operate and comfortably use smart wearable device products, and how “technological anxiety” would affect their behavior and attitude towards using these devices. The variables of “technology readiness”, “technological interactivity”, “perceived usefulness”, “perceived ease of use”, “attitude”, and “intention to use” are therefore discussed in this study. Taking “technological anxiety” as the moderating variable to develop the questionnaire scale, the quantitative research with structural equation model is applied to discuss the older adults’ intention to use smart health wearable devices. The questionnaire was distributed to older adults’ community care centers, senior centers, and senior learning centers in Taiwan, and to an older adults’ group above the age of 60 with experience in using smart bracelets. A total of 200 questionnaires were distributed, and 183 were retrieved, with 166 valid copies. The research results reveal that users with higher technology readiness, and older adult users with higher technological interactivity, present a higher perceived ease of use and perceived usefulness. Technological anxiety would affect users’ attitude and further influence the intention to use. The research results could help understand older adults’ needs for using smart health wearable devices.
Emotion Understanding in Bilingual Preschoolers
Authors: Bukhalenkova Daria , Veraksa Aleksander , Gavrilova Margarita , Kartushina Natalia
Published: April 2022

The effects of bilingualism on child development have been extensively examined in last decades. Research reveals that simultaneous use of two or more languages affects child’s language development, cognitive and social skills. The current study focuses on the so-far understudied theory of emotion understanding in bilingual children. A cohort of 593 bilingual and monolingual 5–6-year-olds took the Russian version of the Test of Emotion Comprehension (TEC) that assesses three components of emotion understanding: emotion understanding of external causes of emotions, reflective causes of emotions; and mental causes of emotions. Our results revealed no group differences between overall emotion understanding and understanding of external and reflective causes of emotions. However, monolingual children had a slightly better understanding of mental causes of emotions compared to bilingual children, when controlling for age, gender, and non-verbal intelligence. These results suggest that children growing up in bilingual environments might require more time and/or language/culture exposure to master the ability to understand mental causes of emotions, taking into account cultural differences, as well as the semantic and lexical differences in emotion labelling and emotion expression in each language.
Implementation of a Life Cycle Cost Deep Learning Prediction Model Based on Building Structure Alternatives for Industrial Buildings
Authors: Meshref Ahmed , El-Dash Karim , Basiouny Mohamed , El-Hadidi Omia
Published: April 2022

Undoubtedly, most industrial buildings have a huge Life Cycle Cost (LCC) throughout their lifespan, and most of these costs occur in structural operation and maintenance costs, environmental impact costs, etc. Hence, it is necessary to think about a fast way to determine the LCC values. Therefore, this article presents an LCC deep learning prediction model to assess structural and envelope-type alternatives for industrial building, and to make a decision for the most suitable structure. The input and output criteria of the prediction model were collected from previous studies. The deep learning network model was developed using a Deep Belief Network (DBN) with Restricted Boltzmann Machine (RBM) hidden layers. Seven investigation cases were studied to validate the prediction model of a 312-item dataset over a period of 30 years, after the training phase of the network to take the suitable hidden layers of the RBM and hidden neurons in each hidden layer that achieved the minimal errors of the model. Another case was studied in the model to compare design structure alternatives, consisting of three main structure frames—a reinforced concrete frame, a precast/pre-stressed concrete frame, and a steel frame—over their life cycle, and make a decision. Precast/pre-stressed concrete frames were the best decision until the end of the life cycle cost, as it is possible to reuse the removed sections in a new industrial building.
Analysis of Youth’s Willingness to Exploit Agribusiness Opportunities in Nigeria with Entrepreneurship as a Moderating Variable
Authors: Ikuemonisan Edamisan Stephen , Mafimisebi Taiwo Ejiola , Ajibefun Igbekele Amos , Akinbola Adeyose Emmanuel , Oladoyin Olanrewaju Peter
Published: April 2022

Despite various initiatives and investments in youth entrepreneurship and start-ups, youth participation in agribusiness has not significantly increased. Therefore, this study analyzed the perceived effects of entrepreneurial traits on youth’s willingness to exploit agribusiness opportunities in Nigeria. A total of 3600 respondents were randomly selected across Nigeria using an online well-structured questionnaire. The data were analyzed using a binomial logistic regression among other tools. The study found that, although only 17% of the respondents were exploiting agribusiness opportunities as of 2020, about 77% of the respondents were willing to exploit agribusiness opportunities in the next 2 years. The study found that entrepreneurial traits had no significant influence on youth’s willingness to exploit agribusiness opportunities until each of the traits was moderated by an individual’s entrepreneurial intention. The implications of the findings are discussed in the study.
A Comparative Analysis of Photon versus Proton Beam Therapy in Neoadjuvant Concurrent Chemoradiotherapy for Intrathoracic Squamous Cell Carcinoma of the Esophagus at a Single Institute
Authors: Choi Jin-Ho , Lee Jong Mog , Kim Moon Soo , Lee Youngjoo , Suh Yang-Gun , Lee Sung Uk , Lee Doo Yeul , Oh Eun Sang , Kim Tae hyun , Moon Sung Ho
Published: April 2022

Background: Proton beam therapy (PBT), as a neoadjuvant chemoradiotherapy (nCRT) modality, is expected to result in better outcomes than photon-based radiotherapy (RT) for esophageal cancer, particularly adenocarcinoma. This study reports the results of nCRT for locally advanced esophageal squamous cell carcinoma (ESCC) using both modalities. Methods: We retrospectively reviewed the records of patients who underwent nCRT for ESCC between 2001 and 2020. A median of 41.4 Gy or cobalt gray equivalents of radiation was delivered using either photons or protons, with concurrent chemotherapy. Dosimetric and clinical parameters were compared between the two groups. Results: Of the 31 patients, the lungs and heart of the proton group (n = 15) were exposed to significantly less radiation compared to the photon group (n = 16). No significant differences in short-term postoperative outcomes or lymphocyte count were observed between the groups, and there were no significant differences between the photon and proton groups in 2-year overall survival (67.8% vs. 68.6%, p = 0.867) or 2-year disease-free survival (33.3% vs. 34.5%, p = 0.749), with a median follow-up of 17 months. Conclusions: PBT provided a significant dosimetric benefit over photon-based RT during nCRT for ESCC; however, it did not improve clinical outcomes.
Implication of COPB2 Expression on Cutaneous Squamous Cell Carcinoma Pathogenesis
Authors: Chen Taiqin , Kim Ki-Yeol , Oh Yeongjoo , Jeung Hei Cheul , Chung Kee Yang , Roh Mi Ryung , Zhang Xianglan
Published: April 2022

The underlying molecular mechanisms of cutaneous squamous cell carcinoma (cSCC) pathogenesis are largely unknown. In the present study, we aimed to evaluate the effect of coatomer protein complex subunit beta 2 (COPB2) expression on cSCC pathogenesis. Clinicopathological significance of COPB2 in cSCC was investigated by analyzing the Gene Expression Omnibus (GEO) database and through a retrospective cohort study of 95 cSCC patients. The effect of COPB2 expression on the biological behavior of cSCC cells was investigated both in vitro and in vivo. We found that COPB2 expression was significantly higher in cSCC samples than in normal skin samples. In our cohort, a considerable association was found between COPB2 expression and indicators of tumor immune microenvironment (TIME), such as histocompatibility complex class (MHC) I, and MHC II, CD4+/ CD8+ tumor-infiltrating lymphocytes. Additionally, COPB2 expression had an independent impact on worsened recurrence-free survival in our cohort. Furthermore, decreased proliferation, invasion, tumorigenic activities, and increased apoptosis were observed after COPB2 knockdown in cSCC cells. COPB2 may act as a potential oncogene and candidate modulator of the TIME in cSCC. Therefore, it can serve as a novel predictive prognostic biomarker and candidate immunotherapeutic target in cSCC patients.
Head and Neck Cancer Susceptibility and Metabolism in Fanconi Anemia
Authors: Chihanga Tafadzwa , Vicente-Muñoz Sara , Ruiz-Torres Sonya , Pal Bidisha , Sertorio Mathieu , Andreassen Paul R. , Khoury Ruby , Mehta Parinda , Davies Stella M. , Lane Andrew N. , Romick-Rosendale Lindsey E. , Wells Susanne I.
Published: April 2022

Fanconi anemia (FA) is a rare inherited, generally autosomal recessive syndrome, but it displays X-linked or dominant negative inheritance for certain genes. FA is characterized by a deficiency in DNA damage repair that results in bone marrow failure, and in an increased risk for various epithelial tumors, most commonly squamous cell carcinomas of the head and neck (HNSCC) and of the esophagus, anogenital tract and skin. Individuals with FA exhibit increased human papilloma virus (HPV) prevalence. Furthermore, a subset of anogenital squamous cell carcinomas (SCCs) in FA harbor HPV sequences and FA-deficient laboratory models reveal molecular crosstalk between HPV and FA proteins. However, a definitive role for HPV in HNSCC development in the FA patient population is unproven. Cellular metabolism plays an integral role in tissue homeostasis, and metabolic deregulation is a known hallmark of cancer progression that supports uncontrolled proliferation, tumor development and metastatic dissemination. The metabolic consequences of FA deficiency in keratinocytes and associated impact on the development of SCC in the FA population is poorly understood. Herein, we review the current literature on the metabolic consequences of FA deficiency and potential effects of resulting metabolic reprogramming on FA cancer phenotypes.
Sugarcane Bagasse Ash as a Catalyst Support for Facile and Highly Scalable Preparation of Magnetic Fenton Catalysts for Ultra-Highly Efficient Removal of Tetracycline
Authors: Rattanachueskul Natthanan , Dokkathin Oraya , Dechtrirat Decha , Panpranot Joongjai , Watcharin Waralee , Kaowphong Sulawan , Chuenchom Laemthong
Published: April 2022

Sugarcane bagasse ash, which is waste from the combustion process of bagasse for electricity generation, was utilized as received as a catalyst support to prepare the magnetic sugarcane bagasse ash (MBGA) with different iron-to-ash ratios using a simple co-precipitation method, and the effects of NaOH and iron loadings on the physicochemical properties of the catalyst were investigated using various intensive characterization techniques. In addition, the catalyst was used with a low amount of H2O2 for the catalytic degradation of a high concentration of tetracycline (800 mg/L) via a Fenton system. The catalyst exhibited excellent degradation activity of 90.43% removal with good magnetic properties and high stabilities and retained good efficiency after four cycles with NaOH as the eluent. Moreover, the hydroxyl radical on the surface of catalyst played a major role in the degradation of TC, and carbon-silica surface of bagasse ash significantly improved the efficiencies. The results indicated that the MBGA catalyst shows the potential to be highly scalable for a practical application, with high performance in the heterogeneous Fenton system.
Low-Temperature O3 Decomposition over Pd-TiO2 Hybrid Catalysts
Authors: Touati Houcine , Mehri Afef , Karouia Fathi , Richard Frédéric , Batiot-Dupeyrat Catherine , Daniele Stéphane , Clacens Jean-Marc
Published: April 2022

In aircraft and spacecraft, outside air is not directly fed to the passenger because it contains ozone at elevated altitudes. The decomposition of low concentration ozone in the air was carried out at 25 °C by catalytic oxidation on Pd-based catalysts supported on a high surface area hybrid TiO2. The use of these hybrid catalysts has shown a beneficial effect, both on the catalytic activity and on the catalyst stability. Kinetic studies showed that the most promising catalytic phase (Pd/TiO2_100) was the one obtained from the TiO2 support containing the lowest content of citrate ligands and leading to small Pd particles (around 4 nm). The effect of catalyst synthesis on the decomposition of O3 gas (15 ppm) in a dry and humid (HR = 10%) stream in a closed environment such as aircraft or spacecraft was also investigated in this study and further elucidated by detailed characterizations. It was shown that the system could be used as an effective treatment for air coming from outside.
Genomic Hippo Pathway Alterations and Persistent YAP/TAZ Activation: New Hallmarks in Head and Neck Cancer
Authors: Faraji Farhoud , Ramirez Sydney I. , Anguiano Quiroz Paola Y. , Mendez-Molina Amaya N. , Gutkind J. Silvio
Published: April 2022

Head and neck squamous cell carcinoma (HNSCC) represents a highly prevalent and deadly malignancy worldwide. The prognosis for locoregionally advanced HNSCC has not appreciably improved over the past 30 years despite advances in surgical, radiation, and targeted therapies and less than 20% of HNSCC patients respond to recently approved immune checkpoint inhibitors. The Hippo signaling pathway, originally discovered as a mechanism regulating tissue growth and organ size, transduces intracellular and extracellular signals to regulate the transcriptional co-activators YAP and TAZ. Alterations in the Hippo pathway resulting in persistent YAP and TAZ activation have emerged as major oncogenic drivers. Our analysis of the human HNSCC oncogenome revealed multiple genomic alterations impairing Hippo signaling and activating YAP and TAZ, which in turn contribute to HNSCC development. This includes mutations and deletions of the FAT1 gene (29%) and amplification of the WWTR1 (encoding TAZ, 14%) and YAP1 genes (8%), together representing one of the most genetically altered signaling mechanisms in this malignancy. Here, we discuss key elements of the mammalian Hippo pathway, detail mechanisms by which perturbations in Hippo signaling promote HNSCC initiation and progression and outline emerging strategies to target Hippo signaling vulnerabilities as part of novel multimodal precision therapies for HNSCC.
Incomplete Recovery of Zebrafish Retina Following Cryoinjury
Authors: Džulová Denisa , Lawless Dylan , Pinton Gaëtan G. , Renner Nicole A. , Schorderet Daniel F.
Published: April 2022

Zebrafish show an extraordinary potential for regeneration in several organs from fins to central nervous system. Most impressively, the outcome of an injury results in a near perfect regeneration and a full functional recovery. Indeed, among the various injury paradigms previously tested in the field of zebrafish retina regeneration, a perfect layered structure is observed after one month of recovery in most of the reported cases. In this study, we applied cryoinjury to the zebrafish eye. We show that retina exposed to this treatment for one second undergoes an acute damage affecting all retinal cell types, followed by a phase of limited tissue remodeling and regrowth. Surprisingly, zebrafish developed a persistent retinal dysplasia observable through 300 days post-injury. There is no indication of fibrosis during the regeneration period, contrary to the regeneration process after cryoinjury to the zebrafish cardiac ventricle. RNA sequencing analysis of injured retinas at different time points has uncovered enriched processes and a number of potential candidate genes. By means of this simple, time and cost-effective technique, we propose a zebrafish injury model that displays a unique inability to completely recover following focal retinal damage; an outcome that is unreported to our knowledge. Furthermore, RNA sequencing proved to be useful in identifying pathways, which may play a crucial role not only in the regeneration of the retina, but in the first initial step of regeneration, degeneration. We propose that this model may prove useful in comparative and translational studies to examine critical pathways for successful regeneration.
Insight into Extracellular Vesicle-Cell Communication: From Cell Recognition to Intracellular Fate
Authors: Ginini Lana , Billan Salem , Fridman Eran , Gil Ziv
Published: April 2022

Extracellular vesicles (EVs) are heterogamous lipid bilayer-enclosed membranous structures secreted by cells. They are comprised of apoptotic bodies, microvesicles, and exosomes, and carry a range of nucleic acids and proteins that are necessary for cell-to-cell communication via interaction on the cells surface. They initiate intracellular signaling pathways or the transference of cargo molecules, which elicit pleiotropic responses in recipient cells in physiological processes, as well as pathological processes, such as cancer. It is therefore important to understand the molecular means by which EVs are taken up into cells. Accordingly, this review summarizes the underlying mechanisms involved in EV targeting and uptake. The primary method of entry by EVs appears to be endocytosis, where clathrin-mediated, caveolae-dependent, macropinocytotic, phagocytotic, and lipid raft-mediated uptake have been variously described as being prevalent. EV uptake mechanisms may depend on proteins and lipids found on the surfaces of both vesicles and target cells. As EVs have been shown to contribute to cancer growth and progression, further exploration and targeting of the gateways utilized by EVs to internalize into tumor cells may assist in the prevention or deceleration of cancer pathogenesis.
Real-Time Fluorescence Imaging of His-Tag-Driven Conjugation of mCherry Proteins to Silver Nanowires
Authors: Jankowska Martyna , Sulowska Karolina , Wiwatowski Kamil , Niedziółka-Jönsson Joanna , Mackowski Sebastian
Published: April 2022

In this work, we aimed to apply fluorescence microscopy to image protein conjugation to Ni-NTA modified silver nanowires in real time via the His-tag attachment. First, a set of experiments was designed and performed for the mixtures of proteins and silver nanowires in order to demonstrate plasmon enhancement of mCherry protein fluorescence as well as the ability to image fluorescence of single molecules. The results indicated strong enhancement of single-protein fluorescence emission upon coupling with silver nanowires. This conclusion was supported by a decrease in the fluorescence decay time of mCherry proteins. Real-time imaging was carried out for a structure created by dropping protein solution onto a glass substrate with functionalized silver nanowires. We observed specific attachment of mCherry proteins to the nanowires, with the recognition time being much longer than in the case of streptavidin–biotin conjugation. This result indicated that it is possible to design a universal and efficient real-time sensing platform with plasmonically active functionalized silver nanowires.
Liver and Pancreatic Involvement in Children with Multisystem Inflammatory Syndrome Related to SARS-CoV-2: A Monocentric Study
Authors: Giannattasio Antonietta , Maglione Marco , D’Anna Carolina , Muzzica Stefania , Pappacoda Serena , Lenta Selvaggia , Di Mita Onorina , Ranucci Giusy , Mandato Claudia , Tipo Vincenzo
Published: April 2022

Liver and pancreatic involvement in children with Multisystem Inflammatory Syndrome related to SARS-CoV-2 (MIS-C) has been poorly investigated so far. We reviewed a cohort of MIS-C patients to analyze the prevalence of acute liver injury (ALI) and pancreatic injury and their correlation with clinical outcomes. Demographic, clinical, laboratory and imaging features of children with MIS-C at admission and during hospital stay were prospectively collected. Fifty-five patients (mean age 6.5 ± 3.7 years) were included. At admission, 16 patients showed ALI and 5 had increased total serum lipase. During observation, 10 more patients developed ALI and 19 more subjects presented raised pancreatic enzymes. In comparison to those with normal ALT, subjects with ALI were significantly older (p = 0.0004), whereas pancreatic involvement was associated to a longer duration of hospital stay compared with patients with normal pancreatic enzymes (p = 0.004). Time between hospital admission and onset of ALI was shorter compared to the onset of raised pancreatic enzymes (3.2 ± 3.9 versus 5.3 ± 2.7 days, respectively; p = 0.035). Abdominal ultrasound showed liver steatosis in 3/26 (12%) and hepatomegaly in 6/26 (16%) patients with ALI; 2 patients presented enlarged pancreas. Although liver and pancreatic involvement is commonly observed in MIS-C patients, it is mild in most cases with a complete recovery.
Strain Engineering of Domain Coexistence in Epitaxial Lead-Titanite Thin Films
Authors: Dong Yanzhe , Lu Xiaoyan , Fan Jinhui , Choi Si-Young , Li Hui
Published: April 2022

Phase and domain structures in ferroelectric materials play a vital role in determining their dielectric and piezoelectric properties. Ferroelectric thin films with coexisting multiple domains or phases often have fascinating high sensitivity and ultrahigh physical properties. However, the control of the coexisting multiple domains is still challenging, thus necessitating the theoretical prediction. Here, we studied the phase coexistence and the domain morphology of PbTiO3 epitaxial films by using a Landau–Devonshire phenomenological model and canonic statistical method. Results show that PbTiO3 films can exist in multiple domain structures that can be diversified by the substrates with different misfit strains. Experimental results for PbTiO3 epitaxial films on different substrates are in good accordance with the theoretical prediction, which shows an alternative way for further manipulation of the ferroelectric domain structures.
Deposition Behavior and Microstructure of Cold-Sprayed Ni-Coated Al Particles
Authors: Chen Xiao , Zhou Hongkai , Pi Zhimin , Huang Zhiwu
Published: April 2022

Cold spraying is a novel technology for preparing solid-state coatings. Single Ni-coated Al particles were deposited onto different substrates by cold spraying at different accelerating gas temperatures, as well as preparing for the coatings. The influence of the accelerating gas temperature and substrate microhardness on the particle deposition deformation, microstructure, and microhardness of Ni-coated Al coatings were investigated. The results show that the embedding depth of Ni-coated Al particles into the Al substrate increased with increasing the accelerating gas temperature. However, the cold-sprayed Ni-coated Al particles did not embed into the Q235 steel substrate, and the degree of plastic deformation of the Ni-coated Al particles increased with increasing the accelerating gas temperature. Moreover, the morphology of the Ni-coated Al splat deposited onto the Q235 steel substrate at an accelerating gas temperature of 400 °C presented a flattened morphology, which was different from the nearly spherical or ellipsoidal morphology of the Ni-coated Al feedstock. Ni-coated Al coatings exhibited the same phase compositions as the feedstock powders, and the Ni and Al phases in the coatings incurred a certain plastic deformation. Compared with the Q235 steel substrate, an Al substrate with a lower microhardness is beneficial for forming the first layer coating, as well as for the formation of an intermixing structure between the Ni-coated Al coating and Al substrate. The porosity of Ni-coated Al coatings decreased and the thickness increased when increasing the gas temperature; in particular, the coating deposited onto Al substrate had the lowest porosity and the largest thickness at an accelerating gas temperature of 400 °C. Meanwhile, the microhardness of the coating deposited onto the Al substrate was higher than that deposited onto the Q235 steel substrate under the same cold spraying conditions.
WO3 Films Grown by Spray Pyrolysis for Smart Windows Applications
Authors: Mouratis Kyriakos , Tudose Ioan Valentin , Romanitan Cosmin , Pachiu Cristina , Popescu Marian , Simistiras Georgios , Couris Stelios , Suchea Mirela Petruta , Koudoumas Emmanuel
Published: April 2022

WO3 nanostructured thin films were grown using spray deposition on FTO coated glass. The effect of the precursor concentration and the solution quantity, which determines the deposition time, on the electrochemical, electrochromic and optical properties of the WO3 films was investigated. The films were found to exhibit a good electrochromic activity with a reasonably good durability of charge exchange and optical modulation under harsh electrochemical cycling in Li-ion-conducting electrolyte. Associated compositional and structural characteristics were probed by several techniques, indicating that the observed improved durability may be due to the unique WO3 thin films’ structuring, the surface of the films consisting of wall-like structures combined with bubble-like islands on a polycrystalline WO3 granular background, that requires further study in greater detail.
Classifying the Degree of Bark Beetle-Induced Damage on Fir (Abies mariesii) Forests, from UAV-Acquired RGB Images
Authors: Leidemer Tobias , Gonroudobou Orou Berme Herve , Nguyen Ha Trang , Ferracini Chiara , Burkhard Benjamin , Diez Yago , Lopez Caceres Maximo Larry
Published: April 2022

Bark beetle outbreaks are responsible for the loss of large areas of forests and in recent years they appear to be increasing in frequency and magnitude as a result of climate change. The aim of this study is to develop a new standardized methodology for the automatic detection of the degree of damage on single fir trees caused by bark beetle attacks using a simple GIS-based model. The classification approach is based on the degree of tree canopy defoliation observed (white pixels) in the UAV-acquired very high resolution RGB orthophotos. We defined six degrees (categories) of damage (healthy, four infested levels and dead) based on the ratio of white pixel to the total number of pixels of a given tree canopy. Category 1: <2.5% (no defoliation); Category 2: 2.5–10% (very low defoliation); Category 3: 10–25% (low defoliation); Category 4: 25–50% (medium defoliation); Category 5: 50–75% (high defoliation), and finally Category 6: >75% (dead). The definition of “white pixel” is crucial, since light conditions during image acquisition drastically affect pixel values. Thus, whiteness was defined as the ratio of red pixel value to the blue pixel value of every single pixel in relation to the ratio of the mean red and mean blue value of the whole orthomosaic. The results show that in an area of 4 ha, out of the 1376 trees, 277 were healthy, 948 were infested (Cat 2, 628; Cat 3, 244; Cat 4, 64; Cat 5, 12), and 151 were dead (Cat 6). The validation led to an average precision of 62%, with Cat 1 and Cat 6 reaching a precision of 73% and 94%, respectively.
Aggregating Composite Indicators through the Geometric Mean: A Penalization Approach
Authors: Mariani Francesca , Ciommi Mariateresa
Published: April 2022

In this paper, we introduce a penalized version of the geometric mean. In analogy with the Mazziotta Pareto Index, this composite indicator is derived as a product between the geometric mean and a penalization term to account for the unbalance among indicators. The unbalance is measured in terms of the (horizontal) variability of the normalized indicators opportunely scaled and transformed via the Box–Cox function of order zero. The penalized geometric mean is used to compute the penalized Human Development Index (HDI), and a comparison with the geometric mean approach is presented. Data come from the Human Development Data Center for 2019 and refer to the classical three dimensions of HDI. The results show that the new method does not upset the original ranking produced by the HDI but it impacts more on countries with poor performances. The paper has the merit of proposing a new reading of the Mazziotta Pareto Index in terms of the reliability of the arithmetic mean as well as of generalizing this reading to the geometric mean approach.
Estimation of the Grüneisen Parameter of High-Entropy Alloy-Type Functional Materials: The Cases of REO0.7F0.3BiS2 and MTe
Authors: Abbas Fysol Ibna , Nakahira Yuki , Yamashita Aichi , Kasem Md. Riad , Yoshida Miku , Goto Yosuke , Miura Akira , Terashima Kensei , Matsumoto Ryo , Takano Yoshihiko , Moriyoshi Chikako , Mizuguchi Yoshikazu
Published: April 2022

In functional materials such as thermoelectric materials and superconductors, the interplay between functionality, electronic structure, and phonon characteristics is one of the key factors to improve functionality and to understand the underlying mechanisms. In the first part of this article, we briefly review investigations on lattice anharmonicity in functional materials on the basis of the Grüneisen parameter (γG). We show that γG can be a good index for large lattice anharmonicity and for detecting a change in anharmonicity amplitude in functional materials. Then, we show original results on the estimation of γG for recently developed high-entropy alloy-type (HEA-type) functional materials with a layered structure and a NaCl-type structure. As a common trend for those two systems with two- and three-dimensional structures, we found that γG increased with a slight increase in the configurational entropy of mixing (ΔSmix) and then decreased with increasing ΔSmix in the high-entropy region.
Robot-Assisted Radical Prostatectomy for Potential Cancer Control in Patients with Metastatic Prostate Cancer
Authors: Takagi Kimiaki , Kawase Makoto , Kato Daiki , Kawase Kota , Takai Manabu , Iinuma Koji , Nakane Keita , Hagiwara Noriyasu , Yamada Toru , Tomioka Masayuki , Koie Takuya
Published: April 2022

Recently, cytoreductive prostatectomy for metastatic prostate cancer (mPCa) has been associated with improved oncological outcomes. This study was aimed at evaluating whether robot-assisted radical prostatectomy (RARP) as a form of cytoreductive prostatectomy can improve oncological outcomes in patients with mPCa. We conducted a retrospective study of twelve patients with mPCa who had undergone neoadjuvant therapy followed by RARP. The endpoints were biochemical recurrence-free survival, treatment-free survival, and de novo metastasis-free survival. At the end of the follow-up period, none of the enrolled patients had died from PCa. The 1- and 2-year biochemical recurrence-free survival rates were 83.3% and 66.7%, respectively, and treatment-free survival rates were 75.0% and 56.3%, respectively. One patient developed de novo bone metastases 6.4 months postoperatively, and castration-resistant prostate cancer 8.9 months postoperatively. After RARP, the median duration of recovery of urinary continence was 5.2 months. One patient had severe incontinence (>2 pads/day) 24 months postoperatively. RARP may be a treatment option in patients with mPCa who have achieved a serum prostate-specific antigen level < 0.2 ng/mL, and present without new lesions on imaging.
Comparative Analysis of Postoperative Complications of Sentinel Node Identification Using the SentiMag® Method and the Use of a Radiotracer in Patients with Breast Cancer
Authors: Lorek Andrzej , Steinhof-Radwańska Katarzyna , Zarębski Wojciech , Lorek Joanna , Stojčev Zoran , Zych Jacek , Syrkiewicz Aleksandra , Niemiec Paweł , Szyluk Karol
Published: April 2022

(1) Background: The purpose of the study was a retrospective, comparative assessment of complications of the surgical sentinel node biopsy (SNB) procedure in breast cancer using the radiotracer method and the SentiMag® method on groups of patients after 3.5 years of use. (2) Methods: The material was a group of 345 patients with primary surgical breast cancer who underwent the SNB procedure with the use of a radiotracer in combination with wide local excision (WLE), simple amputation (SA) with SNB and an independent SNB procedure in the period from May 2018 to January 2021 in the Department of Oncological Surgery. Of the patients who were monitored in the Hospital Outpatient Clinic, 300 were enrolled. The analyzed group was compared in terms of the occurrence of the same complications with the group of 303 patients also operated on in our center in the period from January 2014 to September 2017, in which SN identification was performed using the SentiMag® method. (3) Results: The most common complications found were sensation disorders in the arm, which occurred in 16 (14.1%) patients using the radiotracer method, SentiMag®-11 (9.9%). By comparing the complication rate between the methods with the radiotracer (n = 300) and SentiMag® (n = 303), no significant differences were found. (4) Conclusions: Sentinel node (SN) identification using the radiotracer method and the SentiMag® method are comparable diagnostic methods in breast cancer, with a low risk of complications.
Cryptic Triploids and Leaky Premating Isolation in an Odontophrynus Hybrid Zone
Authors: Martino Adolfo L. , Grenat Pablo R. , Sinsch Ulrich
Published: April 2022

The diploid Odontophrynus cordobae and its autopolyploid counterpart O. americanus (4n) co-occur in a small-sized contact zone in Central Argentina, together with numerous specimens of a cryptic triploid taxon. Additionally, we monitor another five localities inhabited by taxa of this species complex within a radius of 76 km north and south of the contact zone to look for possible co-occurrence of taxa. In this study, we analyze the reproductive interactions in this breeding assemblage covering three levels of ploidy. We focus on the advertisement call structure as a powerful mechanism of premating isolation and on the mating preferences of males and females in the natural habitat. Advertisement calls of triploids were indistinguishable from those of tetraploids, and both differed significantly in pulse rate from diploids. Analyses of 21 amplexi demonstrated that heterospecific matings dominated breeding: one between a diploid female and tetraploid male, and ten between triploids and tetraploids. At three localities with syntopic diploids and tetraploids, premating isolation was almost perfect, preventing the formation of triploids. Therefore, we question the putative origin of triploids from hybridization and discuss alternatives. This unique system, including three bisexual taxa of distinct ploidy, which interact reproductively, remains to be investigated in more detail to fully understand the mechanism stabilizing its persistence.
Diagnosis and Management of Malignant Pleural Effusion: A Decade in Review
Authors: Jacobs Blake , Sheikh Ghias , Youness Houssein A. , Keddissi Jean I. , Abdo Tony
Published: April 2022

Malignant pleural effusion (MPE) is a common complication of thoracic and extrathoracic malignancies and is associated with high mortality. Treatment is mainly palliative, with symptomatic management achieved via effusion drainage and pleurodesis. Pleurodesis may be hastened by administering a sclerosing agent through a thoracostomy tube, thoracoscopy, or an indwelling pleural catheter (IPC). Over the last decade, several randomized controlled studies shaped the current management of MPE in favor of an outpatient-based approach with a notable increase in IPC usage. Patient preferences remain essential in choosing optimal therapy, especially when the lung is expandable. In this article, we reviewed the last 10 to 15 years of MPE literature with a particular focus on the diagnosis and evolving management.
Parental Participation in Intellectual and Developmental Disability Research: A Review of Diversity
Authors: Grove Lauren , Yang Yingying , Diggs Dai’jah , Hershkovich Arielle
Published: April 2022

Parents provide important insights into the psychology, behaviors, and activities of themselves and their children with intellectual and developmental disabilities (IDD). However, it is unknown how prevalent parental participation in IDD research is in general, nor the diversity of geographical locations and research methods of these studies with parental participation. The current review screened 7845 published works in 7 IDD-focused journals between 2010 and 2019. A total of 1519 articles, accounting for 19.37% of all screened works, included at least one parental measure. For each parental article, we coded the country/continent of authors/participants, measurement tools used to obtain data from parents (e.g., questionnaire, interview), and the focus of the measurement (i.e., about parents, child, or both). We found an increasing number and proportion of studies involving parents between 2010 and 2019. Most studies (76%) came from North America and Europe, whereas other continents such as Africa and South America represented less than 1% of all studies reviewed. The predominant (88.0%) measurement tools were questionnaires and interviews. More studies were about both the children and the parents (42.6%) than either only the children (31.3%) or only the parents (26.1%). Together, our review showed a somewhat limited diversity in parental participation in IDD research and these findings have important implications for future research.