bluuebunny/crossref_metadata_2025 huggingface dataset:
Multidimensional Information Network Big Data Mining Algorithm Relying on Finite Element Analysis
Authors: Li Haifeng
Published: April 2022

In recent years, with the rapid development of the Internet, online social networks have been continuously integrated with traditional interpersonal networks and research on information dissemination in social networks has gradually increased. This article studies and analyzes the multidimensional information network big data mining algorithm based on the finite element analysis method. This paper firstly introduces the finite element analysis and calculation process, a finite element data mining simulation application software management system will integrate current data, calculation, and background data into one, then analyzes the data mining clustering algorithm, and conducts an experimental exploration of the influential node mining algorithm in complex networks. The experimental results show that the LIC algorithm is better than the CC algorithm, the DC algorithm, and the BC algorithm; its overall performance is improved by 30%, and the effect is better. The LIC algorithm can effectively and quickly determine the influential nodes, which is helpful for social network analysis.
Antitumor Activity of Royal Jelly and Its Cellular Mechanisms against Ehrlich Solid Tumor in Mice
Authors: Albalawi Aishah E. , Althobaiti Norah A. , Alrdahe Salma S. , Alhasani Reem Hasaballah , Alaryani Fatima S. , BinMowyna Mona N.
Published: April 2022

Objective. The present study was aimed at evaluating the antitumor effects of royal jelly (RJ) obtained from Apis mellifera compared with cyclophosphamide against the Ehrlich solid tumors (EST) in mice. Methods. Tumor growth inhibition, body weight, the serum level of alpha‐fetoprotein (AFP) and carcinoembryonic antigen tumor (CAE), liver and kidney enzymes, tumor lipid peroxidation (LPO), nitric oxide (NO), antioxidant enzymes (glutathione peroxidase (GPx), catalase enzyme (CAT), and superoxide dismutase enzyme activity (SOD)), tumor necrosis factor alpha level (TNF‐α), and the apoptosis‐regulatory genes expression were assessed in EST mice treated with RJ (200 and 400 mg/kg orally once a day for 2 weeks). Results. The results showed that treatment of EST‐suffering mice with RJ at the doses of 200 and 400 mg/kg causes significant reduction in tumor volume and inhibition rate, body weight, tumor markers (AFP and CEA), serum level of liver and kidney, LPO and NO, TNF‐α level, as well as the expression level of Bcl‐2 in comparison with the EST mice receiving the normal saline; whereas RJ at the doses of 200 and 400 mg/kg/day significantly increased (p < 0.05) the level of antioxidant enzymes of GPx, CAT, and SOD and the expression level of caspase‐3 and Bax genes. Conclusion. The findings revealed that oral administration of royal jelly especially at the doses of 200 and 400 mg/kg exhibited promising antitumor effects against EST in mice through induction of apoptosis as well as its antioxidant and anti‐inflammatory effects, which suggest it as a novel anticancer agent against tumor; however, additional surveys especially in clinical setting are necessary to approve these findings.
Electrical Performance Safety Detection of Aramid Casing Based on Molecular Dynamics and Deep Learning Algorithm
Authors: Liu Bowen , Lv Fangcheng , Fan Xiaozhou , Sui Yueyi , Wang Jiaxue , Yin Shengdong
Published: April 2022

Transformer bushing is one of the key equipment of transmission system, and its performance directly affects the stability and safety of transmission system. This paper is aimed at studying the safety detection of electrical properties of aramid shells with molecular dynamics and deep learning algorithms. The bushing needs to withstand AC voltage, DC voltage, and polarity reversal voltage during operation. The complexity of operating conditions leads to the improvement of bushing requirements for insulation performance, and bushing accident is a common type of transformer accident, accounting for 30% of the total number of transformer accidents. Therefore, it is necessary to detect the electrical performance and safety of the bushing to ensure the safe and stable operation of the power system. Aramid casing is a kind of casing with many advantages, such as high strength, high tensile breaking force, high stability, and high temperature resistance. Molecular dynamics is helpful to deeply analyze the micro mechanism of various complex phenomena, so as to explain the relationship between material microstructure and macroproperties, so it is very helpful to analyze the structure and properties of aramid casing. Deep learning is an important research direction in the field of machine learning. It can extract important features and simplify casing analysis steps. In this paper, an electrical performance test system of aramid casing is designed. It is proved that the reliability of casing is generally greater than 1 and the reliability is high. The current performance of the bushing is tested. 400 A is a dividing point, and no matter how large the current is, the maximum temperature is no more than 130°, which proves that the current performance of the bushing is stable and the temperature resistance is good. Finally, the radial field strength distribution of bushing capacitor core under different initial moisture content is tested, and it is concluded that the moisture can not be greater than 6%.
Publication Trends in Rehabilitative Effects of Acupuncture: A Visual Analysis of the Literature
Authors: Zhong Yanmei , Cao Jihui , Lu Haizhen , Huang Zonghai , Liu Lu , Wen Chuanbiao , Chen Ji
Published: April 2022

Objectives. To conduct a comprehensive analysis of scientific outputs in 2011–2021 regarding the rehabilitative effects of acupuncture on diseases. Methods. The study was conducted in the form of knowledge graph and data visualization, with data being drawn from the Web of Science Core Collection database. Results. Articles and reviews were the dominant types; China, Guangzhou University of Chinese Medicine and Medicine ranked was the active country, institution, and journal, respectively, in terms of issued articles. Systematic reviews and the meta-analyses of stroke and pain were extensively carried out in the past decade, whose principal interventions were manual acupuncture, electroacupuncture, scalp acupuncture, and dry needling correspondingly at Baihui (DU20) and Zusanli (ST36). And most frequently utilized rehabilitation assessment criteria were the Fugl-Meyer Assessment Scale and the Barthel Index. More recently, motor function and chronic obstructive pulmonary disease have captured researchers’ attention, which might be the futuristic frontier. Conclusions. This article provided a relatively panoramic picture of the scientific outputs in acupuncture for disease rehabilitation, which may help readers embrace the heated topic and grasp the recent research focus on this field.
Comparison of Two Surgical Approaches to Supination-External Rotation-Type Ankle Fractures
Authors: Chen Bingqian , Wang Zhengfei , Chen Zhi , Qu Xiaohong , Fang Xiaowen , Wang Xuesong , Ke Guoxiu
Published: April 2022

Objective. To compare the clinical outcome and postoperative complications of the treatment of supination-external rotation-type ankle fractures using the posterolateral approach in the prone position and the lateral approach in the supine position. Methods. A retrospective cohort study was conducted in our hospital, including a total of 66 patients ranging from January 2013 to February 2016, regardless of age or sex. All the patients were classified as Lauge-Hansen supination-external rotation (SER)-type ankle fractures and were assigned to receive an open reduction and internal fixation (ORIF) through a posterolateral approach performed in the prone position (the “posterolateral approach group,” 32 patients), or a lateral approach performed in the supine position (the “Lateral Approach Group,” 34 patients). In the posterolateral approach group, 5 patients had Grade II fractures, 8 had Grade III fractures, and 19 had Grade IV fractures; among whom, 12 underwent fixation of the posterior malleolus with hollow screws and 6 with plates, and none of them had fixation of the distal tibiofibular syndesmosis; in the lateral approach group, 4 patients had Grade II fractures, 12 had Grade III fractures, and 18 had Grade IV fractures; among whom, 21 received fixation of the posterior malleolus, and 5 received fixation of the distal tibiofibular syndesmosis. The postoperative complications and ankle scores were recorded. Results. After 1 year of follow-up, in the posterolateral approach group, the incision healed by primary intention and sutures were removed 2 weeks after the operation in all patients; 1 patient had pain in the region posterior to the fibula, presumably peroneal tendon irritation induced by internal fixation, which disappeared after fracture union and fixation device removal; no loosening or breakage of the internal fixation device was reported; the rate of good to excellent results was 96.8% at 12 months. In the lateral approach group: 1 case of wound infection, 1 case of necrosis of lateral skin flap, and 3 cases of lateral skin irritation were reported; the rate of good to excellent results was 94.6% at 12 months. Conclusion. Compared with the traditional lateral approach, the posterolateral approach for the reduction and internal fixation of supination-external rotation-type ankle fractures performed in the prone position has more satisfactory clinical outcomes, with better reduction, more secure fixation, and smaller wound.
Calculation Method of Logistics Energy Consumption in Agricultural Product Supply Chain Based on Structural Equation Model
Authors: Ma Qingxiang
Published: April 2022

The ecological value chain agricultural products in China are mainly produced by scattered farmers. Due to the lack of modern planting and breeding techniques for small-scale scattered farmers, it is difficult to obtain scale effects, and the benefit-cost ratio is generally low. Their own production, supply, and marketing integration organization can only enter the market separately, which is difficult to adapt to market needs, and often bear great market risks. In the circulation link, the agricultural product logistics management level in China is low and logistics losses are huge. This research attempts to conduct a theoretical analysis and discussion on the integration of the ecological agricultural product supply chain in China based on the enterprise theory. At the same time, we conduct an empirical analysis of the relationship between supply chain integration and performance from the perspective of logistics and build agricultural product supply chain integration with the company’s logistics capabilities as the center. We perform hypothesis model, collect data through survey methods, use quantitative analysis and path analysis methods to test the hypothesis model, explore the relationship between supply chain management and agricultural product logistics capabilities, and corporate competitiveness, to provide the empirical support for the introduction of supply chain management in the agriculture in China.
TXNIP: A Double-Edged Sword in Disease and Therapeutic Outlook
Authors: Pan Min , Zhang Fengping , Qu Kai , Liu Chang , Zhang Jingyao
Published: April 2022

Thioredoxin-interacting protein (TXNIP) was originally named vitamin D3 upregulated protein-1 (VDUP1) because of its ability to bind to thioredoxin (TRX) and inhibit TRX function and expression. TXNIP is an alpha-arrestin protein that is essential for redox homeostasis in the human body. TXNIP may act as a double-edged sword in the cell. The balance of TXNIP is crucial. A study has shown that TXNIP can travel between diverse intracellular locations and bind to different proteins to play different roles under oxidative stress. The primary function of TXNIP is to induce apoptosis or pyroptosis under oxidative stress. TXNIP also inhibits proliferation and migration in cancer cells, although TXNIP levels decrease, and function diminishes in various cancers. In this review, we summarized the main structure, binding proteins, pathways, and the role of TXNIP in diseases, aiming to explore the double-edged sword role of TXNIP, and expect it to be helpful for future treatment using TXNIP as a therapeutic target.
Prioritizing and Overcoming Barriers to e-Health Use among Elderly People: Implementation of the Analytical Hierarchical Process (AHP)
Authors: Mumtaz Ayesha
Published: April 2022

Rise in the aging population brings new challenges to modern societies. Old age is associated with several morbidities and usual issues related to health. Therefore, the provision of healthy and timely care has become the dire need to maintain their quality of life and wellbeing. The evolution of the e-health care system put pressure on societies to implement it successfully to ensure a safe and prompt provision of care services to the most vulnerable population successfully. Therefore, the provision and implementation of the e-health care system is a challenge for the health industry in terms of multi-objective decision-making. Multicriteria decision-making is a generalizable approach to making decisions with dependence and feedback and is known as an effective tool in decision-making processes, particularly in the healthcare sector. The present study aims to present an e-healthcare framework by identifying and prioritizing potential barriers towards the use of e-health by the elderly population. The analytical hierarchy process approach is adopted to calculate weights of identified potential barriers, respectively, and then rank them based on their degree of significance. The findings show that health and the ability-related barrier is ranked highest, followed by socio-environmental and attitudinal barriers. This research contributes to healthcare decision-making regarding e-health usage by implementing MCDA techniques. Our study will assist the public health practitioners and policymakers in drawing decisions on the best strategy to minimize the risks in using the e-healthcare system by the aging population, which significantly contributes to the smart healthcare system.
A Method of Monitoring and Evaluating the Quality of English Classroom Teaching Based on Big Data
Authors: Wang Ruili
Published: April 2022

To improve the reliability and accuracy of quality of English instruction in the classroom monitoring and assessment, as well as to reduce the time spent monitoring and evaluating the quality of English instruction in the classroom, a big data-based approach to monitor and evaluate the quality of English instruction in the classroom is proposed. A frequent itemset mining technique for quality of English instruction in the classroom monitoring data is built based on a study of the theoretical basis of big data and the features of quality of English instruction in the classroom monitoring data. To complete the data transformation of quality of English instruction in the classroom monitoring, the multivalued continuous attribute is quantified and turned into a two-dimensional Boolean data matrix. To mine the frequent itemsets of quality of English instruction in the classroom monitoring data, a frequent itemset mining algorithm based on the compressed matrix is applied. This work creates a quality of English instruction in the classroom evaluation model using the gray correlation method of multiobjective decision-making and weighted gray correlation analysis to realize the monitoring and evaluation of quality of English instruction in the classroom. The experimental results suggest that the proposed method is highly reliable and accurate and that it can significantly reduce the time spent monitoring and evaluating the quality of English classroom instruction.
Association between the Expression Levels of MicroRNA-101, -103, and -29a with Autotaxin and Lysophosphatidic Acid Receptor 2 Expression in Gastric Cancer Patients
Authors: Tutunchi Sara , Akhavan Saeedeh , Panahi Ghodratollah , Zare Mina , Emami Razavi Amirnader , Shirkoohi Reza
Published: April 2022

Background. Gastric cancer (GC) is regarded as the most prevalent malignancy with the high mortality rate, worldwide. However, gastroscopy, a biopsy of suspected sample, and detecting CEA, CA19-9, and CA72-4 are presently used, but these diagnostic approaches have several limitations. Recently, microRNAs as the most important member of noncoding RNAs (ncRNAs) have received attention; recent evidence demonstrates that they can be used as the promising candidate biomarkers for GC diagnosis. We aimed to investigate the association between the microRNA-29a, -101, and -103 expression and autotaxin (ATX) and lysophosphatidic acid receptor 2 (LPA2) expression in GC patients. Material and Methods. The present study was conducted on 40 paired samples of primary GC tissue and adjacent noncancerous tissue. The gene expression levels of miR-101, -103, -29, ATX, and LPA2 were analyzed by quantitative reverse-transcription PCR (qRT-PCR). Besides, the protein levels of ATX and LPA2 were evaluated using western blot. Results. The expression levels of miR-29 and miR-101 were significantly lower (

p

value < 0.0001), but the miR-103 and LPA2 were significantly higher in gastric tumor samples compared to the corresponding nontumor tissues (

p

value < 0.0001). Moreover, the diagnostic accuracy of miRs to discrimine the GC patients from noncancerous controls was reliable (miR-101, sensitivity: 82.5% and specificity: 85%; miR-103, sensitivity: 72.5% and specificity: 90%; miR-29, sensitivity: 77.5% and specificity: 70%). Conclusion. It seems that determining the expression level of miR-101, -103, and -29, as the novel diagnostic biomarkers, has diagnostic value to distinguish GC patients from healthy individuals.
An Updated Meta-analysis: Similar Clinical Efficacy of Anterior and Posterior Approaches in Peroral Endoscopic Myotomy (POEM) for Achalasia
Authors: Jing Weina , Luo Xinyue , Yang Jinlin , Wu Junchao , Chen Yuxiang , Deng Kai
Published: April 2022

Introduction. Currently, there are few studies on the efficacy of peroral endoscopic myotomy (POEM) in the anterior or posterior approach; however, limited studies have shown contradictory findings. Thus, the goal was to obtain more quantitative and objective outcomes and further compare the clinical efficacy of these two approaches in this meta-analysis. Methods. A comprehensive search of PubMed, Embase, Cochrane Library, and Web of Science was conducted to find studies relevant to POEM. The retrieval time was from database inception to September 2021. Studies reporting the effects of POEM according to the anterior or posterior approach were included. STATA 16.0 was used to perform statistical analysis, mainly comparing the quantitative objective indicators (lower esophageal sphincter (LES) pressure and Eckardt scores, etc.) in anterior and posterior approaches by meta-analysis. Result. A total of 19 studies with 1261 patients were finally included. Except for shorter procedure time in the posterior approach, other factors (pooled difference of LES pressure, Eckardt scores, clinical success, length of total myotomy, hospital stays, gastroesophageal reflux (GERD), and adverse event) were compared, and all above confirmed that there is no difference between anterior and posterior approaches, and the safety of POEM is ensured. In addition, both anterior and posterior myotomy can improve LES pressure and Eckardt scores, and the difference in anterior and posterior myotomy was unconspicuous. Conclusion. The terms of the pooled difference in LES pressure, Eckardt scores, and other factors (clinical success, length of total myotomy, hospital stays, GERD, adverse events, and procedure time) seemed to be similar for the anterior and posterior approaches. However, the further prognosis after POEM via anterior and posterior approaches needs to be answered in the future.
Land Use Classification Method of Remote Sensing Images for Urban and Rural Planning Monitoring Using Deep Learning
Authors: Xie Xiaoling , Kang Xueqin , Yan Lei , Zeng Liqin , Ye Lin
Published: April 2022

Aiming at the problems that most existing segmentation methods are difficult to deal with the imbalance of remote sensing image distribution and the overlap of segmentation target edges, a land use classification method of remote sensing image for urban and rural planning monitoring based on deep learning is proposed. Firstly, the U-Net is improved by pooling index upsampling and dimension superposition. The improvement can not only extract high-level abstract features but also extract low-level detail features, so as to reduce the loss of image edge information in the process of deconvolution. Then, the batch normalization and scaling exponential linear unit (SeLU) are used to improve the U-Net model. Finally, the improved U-Net model is applied to the classification of remote sensing images of land use types to realize dynamic monitoring. The experimental analysis of the proposed method based on TensorFlow deep learning framework shows that its total accuracy exceeds 94%. The segmentation effect of land use types in remote sensing images is good.
Cushing Syndrome in a Pediatric Patient with Topical Steroid Overuse
Authors: Kuikel Sandip , Aryal Sunil , Basnyat Rupesh Shingh , Rimal Suman
Published: April 2022

Cushing syndrome is a state of hypercortisolism from exogenous or endogenous exposure to glucocorticoids resulting in various clinical manifestations. In this case report, we present a case of a 15-month-old child who presented with cushingoid facies due to over-the-counter misuse of a very potent topical steroid (clobetasol 0.05%) for suspected scabies. Laboratory measurement of urinary free cortisol level was low, and 8 : 00 am basal cortisol level was measured, which was decreased, which confirmed the diagnosis of Cushing syndrome due to exogenous source. Over-the-counter topical steroids should not be used, and one should always consult a registered medical practitioner before using such products. Physicians when prescribing topical steroids should warn patients about the potential side effects of prolonged use of topical steroids.
Defect Point Location Method of Civil Bridge Based on Internet of Things Wireless Communication
Authors: Yan Xiaofeng , Liu Zedong , Zhuang Zijing , Miao Yong
Published: April 2022

With the growth of the country’s comprehensive strength, China’s road and bridge traffic is also growing rapidly. Therefore, the maintenance of highway bridge pavement has become extremely important. The main manifestation of highway bridge deck diseases is bridge deck cracks. If the bridge deck cracks are found in the early stage of damage and solved in time, it will undoubtedly greatly reduce the maintenance cost and care and ensure that the road can be driven safely. At present, the detection of highway bridge defects is mainly based on human vision, but this kind of artificial visual inspection is difficult to complete efficiently. The purpose of the article was to study image recognition techniques and measure the surface damage to bridge superstructures. It has also developed an intelligent software system that can measure and identify cracks under bridges. Aiming at the compatibility problem of wireless communication front end caused by the difference in wireless communication protocols, this study designs a high-applicability front-end control interface for wireless communication. After testing, data can be sent and received when the I/O mode rate drops to 10 Kbps. This method is severely limited and is not suitable for IoT applications with low power consumption and low frequency. It uses the SPI interface for communication and can send and receive normally at different rates, with an upper limit of 8 Mbps. This method consumes a little more pins, but the clock signal is stable, and the transmission performance can meet the needs of most applications.
Is There a Difference in the Diagnosis and Prognosis of Local Recurrence between Autologous Tissue and Implant-Based Breast Reconstruction?
Authors: Min Kyunghyun , Han Hyun Ho , Kim Eun Key , Lee Sae Byul , Kim Jisun , Chung Il Yong , Kim Hee Jeong , Ko Beom Seok , Lee Jong Won , Son Byung Ho , Ahn Sei Hyun , Eom Jin Sup
Published: April 2022

Introduction. Breast reconstruction has become common after total mastectomy; however, certain types of breast reconstruction may be associated with delayed local recurrence or poor survival. Here, we investigated whether there are differences in the diagnosis and prognosis of local recurrence between autologous reconstruction and implant reconstruction. Materials and Methods. A retrospective analysis was performed on patients undergoing breast cancer surgery with autologous tissue or immediate implant reconstruction in a single center (January 2003-December 2017). Patient data including the period from cancer surgery to local recurrence diagnosis, tumor size at the time of recurrence, and survival time after cancer surgery and recurrence detection were analyzed. Results. There was a significant difference (p = 0.021) in the time from surgery to recurrence between the autologous tissue (1,246 days) and implant (909 days) groups. Recurrence tumor size did not differ (autologous: 1.00 cm2 vs. implant: 0.90 cm2; p = 0.813). Survival time after surgery (p = 0.63) and recurrence detection (p = 0.74) did not statistically significant. Conclusions. Statistical difference in the detection time was observed between autologous tissue and implant group. On the other hand, there is no difference in recurrence tumor size or survival time. A further study is necessary to identify the different detection time of local recurrence.
Agricultural Information Management Data Model Construction Based on Cloud Computing and Semantic Technology
Authors: Wang Chunhang , An Zhe
Published: April 2022

In order to improve the data retrieval and mining ability of agricultural information management system, an agricultural information management data model based on cloud computing and semantic technology was proposed. Fuzzy C-means algorithm is used for adaptive fusion and clustering of semantic association features of distributed data in large-scale information management systems; feature compressors are used to reduce the storage space dimension of large-scale information management system to improve the target data mining capability and adaptive scheduling capability of information management system. The registration rate improves than the traditional method by 12.46% and computational cost by 23.76%. This method has higher accuracy in storage data mining and retrieval for large information management systems and superior performance than the traditional method. The introduction of the concept and technical methods of related data is the best practice to realize the fine disclosure, standardized description, semantic organization, and in-depth integration of massive agricultural science and technology information resources, which will play an important role in improving the visibility, visibility, and accessibility of agricultural information resources. It is innovative in the construction of the multidimensional semantic-related model and field knowledge service system driven by related data.
Basic Principles of Electrospinning, Mechanisms, Nanofibre Production, and Anticancer Drug Delivery
Authors: Odularu Ayodele Temidayo
Published: April 2022

Electrospun nanofibres are environmentally friendly compounds, when compared with other approaches of manufacturing nanofibres. This study reviews an easy and simple approach process of producing nanofibres called electrospinning. This review further gives an overview and successful methodical approaches to obtain electrospun (ES) nanofibres appropriate for anticancer drug delivery. The properties and characterization of electrospun nanofibres were reported to confirm successful nanofibre production. The application of characterized ES nanofibres is to deliver the anticancer drug to the right target in the human body. The implication of this study is the application of some of the merits of ES nanofibres (biocompatability, biodegradability, low-cost production, small pore size, and ability to transport anticancer drug to the target cell or organ) to overcome the challenges experienced in the use of anticancer chemotherapeutic agents.
Naoluo Xintong Decoction Ameliorates Cerebral Ischemia-Reperfusion Injury by Promoting Angiogenesis through Activating the HIF-1α/VEGF Signaling Pathway in Rats
Authors: Li Pei-Pei , He Ling , Zhang Li-Miao , Qin Xue-Mei , Hu Jian-Peng
Published: April 2022

Background. Naoluo Xintong decoction (NLXTD) is a traditional Chinese medicine (TCM) formula which has been used to improve neuronal functional recovery after cerebral ischemic stroke. However, the molecular mechanism underlying NLXTD’s amelioration of ischemic stroke remains unclear. The present study was designed to explore the effect and mechanism of NLXTD on brain angiogenesis in a rat model with cerebral ischemia-reperfusion (I/R) injury targeting the hypoxia-inducible factor-1α (HIF-1α)/vascular endothelial growth factor (VEGF) pathway. Materials and Methods. Cerebral I/R model was established by the classical middle cerebral artery occlusion (MCAO) method. Sprague-Dawley (SD) male rats (n = 80) were randomly divided into the sham-operation group, the model group, the HIF-1α inhibitor 2-methoxyestradiol (2ME2) group, the 2ME2 with NLXTD group, and the NLXTD group. Neurological deficit test, TTC staining, H&E staining, TUNEL staining, immunohistochemistry (IH), immunofluorescence (IF), western blot, and quantitative RT-PCR were performed to evaluate the effect of NLXTD after MCAO. Results. Administration of NLXTD significantly decreased neuron deficiency scores, reduced brain infarct volume, and lowered damaged and apoptotic cells after brain I/R injury in rats. Meanwhile, NLXTD had a protective effect on angiogenesis by increasing the MVD and the expressions of BrdU and CD34, which enhanced the number of endothelial cells in the ischemic penumbra brain. NLXTD treatment significantly raised the protein and mRNA levels of HIF-1α, VEGF, VEGFR2, and Notch1 compared with the model treatment. In contrast, a specific HIF-1α inhibitor, 2ME2, inhibited the improvement of neurological function and angiogenesis in NLXTD-induced rats with cerebral I/R injury, suggesting that NLXTD played a positive role in ischemic brain injury by activating the HIF-1α/VEGF signaling pathway. Conclusions. NLXTD exerts neuroprotection targeting angiogenesis by upregulating the HIF-1α/VEGF signaling pathway on cerebral I/R injury rats.
The Combination of Zerumbone with 5-Fluorouracil for Sensitizing Colorectal Cancer-Associated Fibroblasts to Treatment
Authors: Nobari Sima , Najafi Rezvan , Mahdavinezhad Ali , jalali Akram , Amini Razieh
Published: April 2022

The present study aimed to evaluate the synergic effects of combination therapy on 5-fluorouracil (5-FU) resistance-cancer-associated fibroblasts (CAFs) to treatment. Chemotherapy resistance is an important challenge in colorectal cancer (CRC) eradication attention to the tumor microenvironment (TME) is very important. CAFs in the TME play an essential role in cancer chemoresistance and relapse. Additionally, many patients with advanced CRC show resistance to 5-FU therapy. Anti-tumorigenic activities of ZER, a chemopreventive compound derived from the rhizomes of the wild ginger, have been demonstrated. Synergistic and potentiating effects of combination therapy, using herbal and chemical drugs, can improve patients’ response. At the first, CAFs were isolated from a CRC patient and sorted by fluorescent-activated cell sorting (FACS), then, confirmed by flow cytometry, and immunocytochemistry (ICC). The effect of 5-FU and ZER on the cell viability was investigated by MTT assay in a dose and time-dependent manner, after that, the expression of vimentin, β-catenin, and survivin was quantified. Apoptosis, cell cycle, and invasion were analyzed by flow cytometry and scratch test, respectively. ZER could significantly sensitize CAFs cells to 5-FU. A combination of 5-FU + ZER revealed a marked decrease in the marker of interest in both mRNA and protein levels compared to control groups, including 5-FU, ZER treated, and untreated cells. Functional evaluation of cells in different groups presented significant suppression in migration of CAFs and an apparent increase in cell arrest and apoptosis by 5-FU + ZER treatment.
UBE2D1 and COX7C as Potential Biomarkers of Diabetes‐Related Sepsis
Authors: Wang Xin , Wang Lan-tao , Yu Bin
Published: April 2022

Patients with diabetes are physiologically frail and more likely to suffer from infections and even life‐threatening sepsis. This study aimed to identify and verify potential biomarkers of diabetes‐related sepsis (DRS). Datasets GSE7014, GSE57065, and GSE95233 from the Gene Expression Omnibus were used to explore diabetes‐ and sepsis‐related differentially expressed genes (DEGs). Gene set enrichment analysis (GSEA) and functional analyses were performed to explore potential functions and pathways associated with sepsis and diabetes. Weighted gene co‐expression network analysis (WGCNA) was performed to identify diabetes‐ and sepsis‐related modules. Functional enrichment analysis was performed to determine the characteristics and pathways of key modules. Intersecting DEGs that were also present in key modules were considered as common DEGs. Protein‐protein interaction (PPI) network and key genes were analyzed to screen hub genes involved in DRS development. A mouse C57 BL/6J‐DRS model and a neural network prediction model were constructed to verify the relationship between hub genes and DRS. In total, 7457 diabetes‐related DEGs and 2606 sepsis‐related DEGs were identified. GSEA indicated that gene datasets associated with diabetes and sepsis were mainly enriched in metabolic processes linked to inflammatory responses and reactive oxygen species, respectively. WGCNA indicated that grey60 and brown modules were diabetes‐ and sepsis‐related key modules, respectively. Functional analysis showed that grey60 module genes were mainly enriched in cell morphogenesis, heart development, and the PI3K‐Akt signaling pathway, whereas genes from the brown module were mainly enriched in organelle inner membrane, mitochondrion organization, and oxidative phosphorylation. UBE2D1, IDH1, DLD, ATP5C1, COX6C, and COX7C were identified as hub genes in the PPI network. Animal DRS and neural network prediction models indicated that the expression levels of UBE2D1 and COX7C in DRS models and samples were higher than control mice. UBE2D1 and COX7C were identified as potential biomarkers of DRS. These findings may help develop treatment strategies for DRS.
The Value of Serum Exosomal miR-184 in the Diagnosis of NSCLC
Authors: Li Shujun , Lin Yanming , Wu Yanxia , Chen Hualin , Huang Zhong , Lin Muwen , Dong Jiali , Wang Yongcun , Yang Zhixiong
Published: April 2022

Lung cancer has the highest morbidity rate (11.6%) and mortality rate (18.4%) among all current tumors. The morbidity rate in China accounts for approximately one-third, and it is still rising. Nonsmall cell lung cancer is the most common type of lung cancer, accounting for 80%–85% of all lung cancers, and approximately 57% of patients with advanced nonsmall cell lung cancer have distant metastases at the time of diagnosis. To explore the expression changes in microRNA-184 (miR-184) and its clinical value in serum exosomes of patients with nonsmall cell lung cancer (NSCLC). This study adopted a case-control study method, selecting 88 patients (NSCLC group) from June 2015 to June 2017 in our hospital who are confirmed to have NSCLC by fiber-optic bronchoscopy, and 90 patients who are confirmed to have benign lung diseases by pathological examination during the same period (control group). Fluorescence quantitative PCR technology is used to detect the levels of miR-184 in serum exosomes of the two groups, and the differences in the levels of miR-184 in serum exosomes of NSCLC patients with different pathological characteristics are analyzed. According to the results of the 3-year follow-up, the miR-184 levels in serum exosomes of NSCLC patients are grouped and compared. The expression level of miR-184 in serum exosomes in the NSCLC group is significantly higher than that in the control group, and the difference between the two groups is statistically significant (

p

 < 0.05). The ROC curve is drawn with the expression level of miR-184 in serum exosomes of the two groups of patients. The results showed that the area under the ROC curve for the differential diagnosis of NSCLC and benign lung tumors with the expression level of miR-184 in serum exosomes is 0.927, and the sensitivity is 87.61%, while the specificity is 84.02%. The expression levels of miR-184 in serum exosomes of NSCLC patients with different pathological characteristics, in different TNM stages [(I+II) vs. (III+IV)], lymph node metastasis (yes vs. no), and different degrees of differentiation [(High + Medium) vs. Poorly differentiated] are compared and showed statistical significance (

p

 < 0.05). In 88 NSCLC patients, after 3 years of follow-up, 33 survived, and 55 died, with a survival rate of 37.50%. The expression of miR-184 in serum exosomes of the 33 surviving patients is significantly lower than that of the nonsurviving group (

p

 < 0.05). The expression level of miR-184 in serum exosomes of NSCLC patients is significantly increased, which has a certain value for the differential diagnosis of the nature of benign and malignant lung diseases and is closely related to the prognosis of patients.
Experimental Study on Corrosion of Galfan‐Coated Full‐Locked Coil Ropes in a Natatorium Environment
Authors: Zhang Shi-Chang , Xu Xiao-Ming , Gao Feng , Luo Bin , Shi Wei-Zhou , Fang Qing
Published: April 2022

Galfan‐coated full‐locked coil rope is widely applied in long‐span spatial structures because of its high strength and lightweight. However, the corrosion problem is relatively serious in the natatorium, which affects the structure’s durability. To deeply reveal the corrosion mechanism of Galfan‐coated cable, this study first measured the natatorium on‐site, and the results indicated that the natatorium environment can be simulated by a constant temperature and humidity environment in engineering. Then, the constant temperature and humidity corrosion test was carried out to simulate the corrosion of Galfan‐coated cable with a certain amount of chloride ions on the surface, and the early corrosion rate was obtained. Later, the neutral salt spray accelerated corrosion test was carried out to predict the corrosion rate of the cable in the natatorium environment in the middle and late periods. The specimens included no‐stress unprotected coating cable, high‐stress unprotected coating cable, and high‐stress protected coating cable. The results show that high stress will accelerate the corrosion, and a protective coating can effectively prevent the corrosion. Finally, the linear model, bilinear model, and bilinear model considering chloride ion accumulation were proposed to study the corrosion of the Galfan coating. The results show that the linear model overestimates the corrosion rate, and the bilinear model and bilinear model considering chloride ion accumulation are practical. Meanwhile, corresponding maintenance suggestions were given for the natatorium project to improve the structure's durability.
Reduced Graphene Oxide‐Tailored CuFe2O4 Nanoparticles as an Electrode Material for High‐Performance Supercapacitors
Authors: Mary B. Carmel Jeeva , Vijaya J. Judith , Nair Radhika R. , Mustafa A. , Selvamani P. Stephen , Saravanakumar B. , Bououdina M. , Kennedy L. John
Published: April 2022

Transition metal oxide‐based magnetic nanocomposites attract great attention due to their unique properties and applications in the field of energy storage. Herein, we present a facile microwave procedure for the synthesis of CuFe2O4 (CF) and CuFe2O4 incorporated with reduced graphene oxide CuFe2O4/rGO (CG) as potential electrode materials for hybrid supercapacitor. The structure and morphology of CF and CG nanoparticles are examined. The electrochemical performance is studied in 6 M aqueous KOH electrolyte using cyclic voltammetry (CV), galvanostatic charge‐discharge (GCD), and electrochemical impedance spectroscopy (EIS) techniques. The attractive CG nanocomposite exhibits high specific capacity of 800 C/g at a current density of 2 A/g and better cycling stability when compared to pure CF, due to the formation of nanostructure composed of ferrite nanoparticles homogeneously incorporated onto rGO sheets. Furthermore, the practicability of CG electrode is investigated by the fabrication of CG and activated carbon. The hybrid supercapacitor device shows excellent electrochemical performance with specific energy of 18.3 Wh/kg and a specific power of 455 W/kg. It is noteworthy that the cyclic stability is excellent with a capacity retention of ~98% after 3000 cycles manifesting the superiority of CG electrode. The proposed device demonstrates the potential to fabricate other metal oxides with activated carbon via a facile synthesis method for promoting application in energy storage materials and promoting new opportunities of binary nanocomposite.
Loss of KMT5C Promotes EGFR Inhibitor Resistance in NSCLC via LINC01510-Mediated Upregulation of MET
Authors: Pal Arpita S. , Agredo Alejandra , Lanman Nadia A. , Son Jihye , Sohal Ikjot Singh , Bains Manvir , Li Chennan , Clingerman Jenna , Gates Kayla , Kasinski Andrea L.
Published: April 2022

EGFR inhibitors (EGFRi) are standard-of-care treatments administered to patients with non–small cell lung cancer (NSCLC) that harbor EGFR alterations. However, development of resistance posttreatment remains a major challenge. Multiple mechanisms can promote survival of EGFRi-treated NSCLC cells, including secondary mutations in EGFR and activation of bypass tracks that circumvent the requirement for EGFR signaling. Nevertheless, the mechanisms involved in bypass signaling activation are understudied and require further elucidation. In this study, we identify that loss of an epigenetic factor, lysine methyltransferase 5C (KMT5C), drives resistance of NSCLC to multiple EGFRis, including erlotinib, gefitinib, afatinib, and osimertinib. KMT5C catalyzed trimethylation of histone H4 lysine 20 (H4K20), a modification required for gene repression and maintenance of heterochromatin. Loss of KMT5C led to upregulation of an oncogenic long noncoding RNA, LINC01510, that promoted transcription of the oncogene MET, a component of a major bypass mechanism involved in EGFRi resistance. These findings underscore the loss of KMT5C as a critical event in driving EGFRi resistance by promoting a LINC01510/MET axis, providing mechanistic insights that could help improve NSCLC treatment.

Significance:

Dysregulation of the epigenetic modifier KMT5C can drive MET-mediated EGFRi resistance, implicating KMT5C loss as a putative biomarker of resistance and H4K20 methylation as a potential target in EGFRi-resistant lung cancer.
A Genome-Wide CRISPR Activation Screen Identifies PRRX2 as a Regulator of Enzalutamide Resistance in Prostate Cancer
Authors: Rodríguez Yara , Unno Kenji , Truica Mihai I. , Chalmers Zachary R. , Yoo Young A. , Vatapalli Rajita , Sagar Vinay , Yu Jindan , Lysy Barbara , Hussain Maha , Han Huiying , Abdulkadir Sarki A.
Published: April 2022

Androgen receptor (AR) pathway inhibitors are the mainstay treatment for advanced prostate cancer, but resistance to therapy is common. Here, we used a CRISPR activation screen in metastatic castration-sensitive prostate cancer cells to identify genes that promote resistance to AR inhibitors. Activation of the TGFβ target gene paired-related homeobox2 (PRRX2) promoted enzalutamide resistance. PRRX2 expression was the highest in double-negative prostate cancer (DNPC), which lack AR signaling and neuroendocrine differentiation, and a PRRX2-related gene signature identified a subset of patients with DNPC with reduced overall survival. PRRX2-expressing cells showed alterations in the CDK4/6/Rb/E2F and BCL2 pathways. Accordingly, treatment with CDK4/6 and BCL2 inhibitors sensitized PRRX2-expressing, castration-resistant tumors to enzalutamide. Overall, PRRX2 was identified as a driver of enzalutamide resistance. The PRRX2 signature merits investigation as a biomarker of enzalutamide resistance in prostate cancer that could be reversed with CDK4/6 and BCL2 inhibitors.

Significance:

PRRX2 mediates enzalutamide resistance via activation of the E2F and BCL2 pathways, which can be targeted with CDK4/6 and BCL2 inhibitors to reverse resistance.
B7-H3 Specific CAR T Cells for the Naturally Occurring, Spontaneous Canine Sarcoma Model
Authors: Zhang Shihong , Black R. Graeme , Kohli Karan , Hayes Brian J. , Miller Cassandra , Koehne Amanda , Schroeder Brett A. , Abrams Kraig , Schulte Brian C. , Alexiev Borislav A. , Heimberger Amy B. , Zhang Ali , Jing Weiqing , Ng Juliana Chi Kei , Shinglot Himaly , Seguin Bernard , Salter Alexander I. , Riddell Stanley R. , Jensen Michael C. , Gottschalk Stephen , Moore Peter F. , Torok-Storb Beverly , Pollack Seth M.
Published: April 2022

One obstacle for human solid tumor immunotherapy research is the lack of clinically relevant animal models. In this study, we sought to establish a chimeric antigen receptor (CAR) T-cell treatment model for naturally occurring canine sarcomas as a model for human CAR T-cell therapy.

Canine CARs specific for B7-H3 were constructed using a single-chain variable fragment derived from the human B7-H3–specific antibody MGA271, which we confirmed to be cross-reactive with canine B7-H3. After refining activation, transduction, and expansion methods, we confirmed target killing in a tumor spheroid three-dimensional assay. We designed a B7-H3 canine CAR T-cell and achieved consistently high levels of transduction efficacy, expansion, and in vitro tumor killing. Safety of the CAR T cells were confirmed in two purposely bred healthy canine subjects following lymphodepletion by cyclophosphamide and fludarabine. Immune response, clinical parameters, and manifestation were closely monitored after treatments and were shown to resemble that of humans. No severe adverse events were observed.

In summary, we demonstrated that similar to human cancers, B7-H3 can serve as a target for canine solid tumors. We successfully generated highly functional canine B7-H3–specific CAR T-cell products using a production protocol that closely models human CAR T-cell production procedure. The treatment regimen that we designed was confirmed to be safe in vivo. Our research provides a promising direction to establish in vitro and in vivo models for immunotherapy for canine and human solid tumor treatment.