bluuebunny/crossref_metadata_2025 huggingface dataset:
Self-attention random forest for breast cancer image classification
Authors: Li Jia , Shi Jingwen , Chen Jianrong , Du Ziqi , Huang Li
Published: February 2023

Introduction

Early screening and diagnosis of breast cancer can not only detect hidden diseases in time, but also effectively improve the survival rate of patients. Therefore, the accurate classification of breast cancer images becomes the key to auxiliary diagnosis.

Methods

In this paper, on the basis of extracting multi-scale fusion features of breast cancer images using pyramid gray level co-occurrence matrix, we present a Self-Attention Random Forest (SARF) model as a classifier to explain the importance of fusion features, and can perform adaptive refinement processing on features, thus, the classification accuracy can be improved. In addition, we use GridSearchCV technique to optimize the hyperparameters of the model, which greatly avoids the limitation of artificially selected parameters.

Results

To demonstrate the effectiveness of our method, we perform validation on the breast cancer histopathological image-BreaKHis. The proposed method achieves an average accuracy of 92.96% and a micro average AUC value of 0.9588 for eight-class classification, and an average accuracy of 97.16% and an AUC value of 0.9713 for binary classification on BreaKHis dataset.

Discussion

For the sake of verify the universality of the proposed model, we also conduct experiments on MIAS dataset. An excellent average classification accuracy is 98.79% on MIAS dataset. Compared to other state-of-the-art methods, the experimental results demonstrate that the performance of the proposed method is superior to that of others. Furthermore, we can analyze the influence of different types of features on the proposed model, and provide theoretical basis for further optimization of the model in the future.
Adverse reactions after treatment with dasatinib in chronic myeloid leukemia: Characteristics, potential mechanisms, and clinical management strategies
Authors: Cheng Fang , Xu Qiling , Li Qiang , Cui Zheng , Li Weiming , Zeng Fang
Published: February 2023

Dasatinib, a second-generation tyrosine kinase inhibitor, is recommended as first-line treatment for patients newly diagnosed with chronic myeloid leukemia (CML) and second-line treatment for those who are resistant or intolerant to therapy with imatinib. Dasatinib is superior to imatinib in terms of clinical response; however, the potential pulmonary toxicities associated with dasatinib, such as pulmonary arterial hypertension and pleural effusion, may limit its clinical use. Appropriate management of dasatinib-related severe events is important for improving the quality of life and prognosis of patients with CML. This review summarizes current knowledge regarding the characteristics, potential mechanisms, and clinical management of adverse reactions occurring after treatment of CML with dasatinib.
Effects of acetazolamide on linear growth in children with pseudotumor cerebri syndrome
Authors: Sheldon Claire A. , Reggie Sara N. , Paley Grace L. , McCormack Shana E. , Liu Grant T.
Published: February 2023

Background/introduction

While oral acetazolamide is a cornerstone of management of adult and pediatric PTCS, previous studies have suggested that acetazolamide used in children with other conditions may influence growth.

Aims and methods

Retrospective chart review involving a single tertiary medical center. Thirty-four children with definite or probable PTCS were identified. Analysis was restricted to individuals from whom anthropometric data were available before and during acetazolamide treatment (n=22).

Results

Half of individuals (n=11/22) showed a decline in BMI Z-scores. Sixty-three percent (n=14/22) showed a decrease in height Z-scores during treatment with acetazolamide; in 6 of these 14 children who had complete data, 3 showed at least a partial recovery of height Z-scores after discontinuation of acetazolamide.

Conclusion

Acetazolamide may be associated with growth suppression in some children treated for pediatric PTCS. In some cases, the growth suppression appears to reverse once the acetazolamide is stopped.
Management of respiratory tract exacerbations in people with cystic fibrosis: Focus on imaging
Authors: Landini Nicholas , Ciet Pierluigi , Janssens Hettie M. , Bertolo Silvia , Ros Mirco , Mattone Monica , Catalano Carlo , Majo Fabio , Costa Stefano , Gramegna Andrea , Lucca Francesca , Parisi Giuseppe Fabio , Saba Luca , Tiddens Harm A. W. M. , Morana Giovanni
Published: February 2023

Respiratory tract exacerbations play a crucial role in progressive lung damage of people with cystic fibrosis, representing a major determinant in the loss of functional lung tissue, quality of life and patient survival. Detection and monitoring of respiratory tract exacerbations are challenging for clinicians, since under- and over-treatment convey several risks for the patient. Although various diagnostic and monitoring tools are available, their implementation is hampered by the current definition of respiratory tract exacerbation, which lacks objective “cut-offs” for clinical and lung function parameters. In particular, the latter shows a large variability, making the current 10% change in spirometry outcomes an unreliable threshold to detect exacerbation. Moreover, spirometry cannot be reliably performed in preschool children and new emerging tools, such as the forced oscillation technique, are still complementary and need more validation. Therefore, lung imaging is a key in providing respiratory tract exacerbation-related structural and functional information. However, imaging encompasses several diagnostic options, each with different advantages and limitations; for instance, conventional chest radiography, the most used radiological technique, may lack sensitivity and specificity in respiratory tract exacerbations diagnosis. Other methods, including computed tomography, positron emission tomography and magnetic resonance imaging, are limited by either radiation safety issues or the need for anesthesia in uncooperative patients. Finally, lung ultrasound has been proposed as a safe bedside option but it is highly operator-dependent and there is no strong evidence of its possible use during respiratory tract exacerbation. This review summarizes the clinical challenges of respiratory tract exacerbations in patients with cystic fibrosis with a special focus on imaging. Firstly, the definition of respiratory tract exacerbation is examined, while diagnostic and monitoring tools are briefly described to set the scene. This is followed by advantages and disadvantages of each imaging technique, concluding with a diagnostic imaging algorithm for disease monitoring during respiratory tract exacerbation in the cystic fibrosis patient.
Choroidal thickness and vascular microstructure parameters in Chinese school-age children with high hyperopia using optical coherence tomography
Authors: Zhu Dehai , Wang Hui , Li Ruoshi , Wen Jing , Li Ruiying , Zhao Jingjing
Published: February 2023

Background

The current study was to evaluate the choroidal thickness (CT) and vascular microstructure parameters in Chinese children with high hyperopia through enhanced depth imaging optical coherence tomography (EDI-OCT).

Methods

Cross-sectional study. A total of 23 children with high hyperopia and 29 children with normal refractive status were retrospectively enrolled in the study. The measurement of the macular CT, 7 points: the sub-foveal area point, the temporal and nasal points at a radius of 0.5-mm, 1.5-mm, and 3-mm were measured. After binarization of the OCT images, the total choroidal area (TCA), stromal area (SA) as well as the luminal area (LA) were identified and measured. The choroidal vascularity index (CVI) was defined as the ratio of LA to TCA. The independent t-test for normal distributions and Kruskal-Wallis tests for non-normal distributions were used to compare other parameters between groups. The Tamhane's T2 test was performed to adjust for multiple comparisons between groups within each analysis.

Results

The subfoveal CT (SFCT) in the high hypermetropic group was significantly thicker than that in normal controls (309.22 ± 53.14 μm vs. 291.27 ± 38.27 μm; P = 0.019). At 0.5 mm, 1.5 mm, and 3.0 mm in diameter, the nasal choroidal sectors of the high hyperopia eyes were significantly thicker than that of the control (P < 0.05). There was significant difference in the choroidal vascular parameters. TCA and LA in the high hyperopia eyes was significantly larger than that of the normal control eyes (3078129.54 ± 448271.18 μm2 vs. 2765218.17 ± 317827.19 μm2, 1926819.54 ± 229817.56 μm2 vs. 1748817.18 ± 191827.98 μm2; P = 0.009, P = 0.011; Table 2). SA values were 1086287.55 ± 212712.11 um2 in the high hyperopia eyes and 999712.71 ± 209838.12 μm2 in the control eyes. The CVI and LA/SA ratio values were differed significantly in the two groups (P = 0.019, P = 0.030, respectively). AL was significantly correlated with SFCT (r = −0.325, P = 0.047), but not significantly correlated with other parameters. Spherical equivalent (SE) was significantly correlated with AL and SFCT (r = −0.711, r = 0.311; P = 0.001, P = 0.016), whereas no significant association between sphere and other parameters.

Conclusion

The choroidal structure of the high hyperopia eyes was different from the normal control eyes. The thicker SFCT, higher LA, and TCA were characteristic of high hyperopia eyes. Choroidal blood flow may be decreased in amblyopic eyes. SFCT of high hyperopia children abnormally increased and correlated with shorter AL and higher SE. AL and SE affect choroidal structure and vascular density.
Function of sildenafil on diseases other than urogenital system: An umbrella review
Authors: Chen Zeyu , Huang Yin , Cao Dehong , Qiu Shi , Chen Bo , Li Jin , Bao Yige , Wei Qiang , Han Ping , Liu Liangren
Published: February 2023

Background: To investigate the function of sildenafil on diseases other than urogenital system, an umbrella review was conducted.

Methods: Meta-analysis and systematic reviews on this topic were comprehensively evaluated in this umbrella review. Quality of evidence was evaluated through AMSTAR and the Grading of Recommendations, Assessment, Development and Evaluation system to generate a reliable and valid conclusion.

Results: 77 out of 1164 meta-analysis were enrolled. 33 significant outcomes and 41 non-significant outcomes were extracted from all eligible articles. We found sildenafil did significant help in reducing arterial systolic pressure, mean pulmonary arterial pressure, pulmonary arterial pressure, systolic pulmonary arterial pressure in patients with pulmonary and cardiovascular diseases. Besides, sildenafil also improved exercise capacity or performance in patients with pulmonary and cardiovascular diseases. Other than these patients, this drug contributed great help in pregnant women with fetal growth restriction and preeclampsia by increasing the weight of newborns and lowering uterine and umbilical pulsatility indices. Additionally, it was reported that utilization of sildenafil has brought increased risk of melanoma.

Conclusion: We can conclude from our study that sildenafil played an important role in many fields, especially in vascular protection. This finding provides a strong evidence for further expansion of sildenafil utilization in other diseases.
Renal function protection and the mechanism of ginsenosides: Current progress and future perspectives
Authors: Fan Meiling , Lan Xintian , Wang Qunling , Shan Mengyao , Fang Xiaoxue , Zhang Yegang , Wu Donglu , Luo Haoming , Gao Wenyi , Zhu Difu
Published: February 2023

Nephropathy is a general term for kidney diseases, which refers to changes in the structure and function of the kidney caused by various factors, resulting in pathological damage to the kidney, abnormal blood or urine components, and other diseases. The main manifestations of kidney disease include hematuria, albuminuria, edema, hypertension, anemia, lower back pain, oliguria, and other symptoms. Early detection, diagnosis, and active treatment are required to prevent chronic renal failure. The concept of nephropathy encompasses a wide range of conditions, including acute renal injury, chronic kidney disease, nephritis, renal fibrosis, and diabetic nephropathy. Some of these kidney-related diseases are interrelated and may lead to serious complications without effective control. In serious cases, it can also develop into chronic renal dysfunction and eventually end-stage renal disease. As a result, it seriously affects the quality of life of patients and places a great economic burden on society and families. Ginsenoside is one of the main active components of ginseng, with anti-inflammatory, anti-tumor, antioxidant, and other pharmacological activities. A variety of monomers in ginsenosides can play protective roles in multiple organs. According to the difference of core structure, ginsenosides can be divided into protopanaxadiol-type (including Rb1, Rb3, Rg3, Rh2, Rd and CK, etc.), and protopanaxatriol (protopanaxatriol)- type (including Rg1, Rg2 and Rh1, etc.), and other types (including Rg5, Rh4, Rh3, Rk1, and Rk3, etc.). All of these ginsenosides showed significant renal function protection, which can reduce renal damage in renal injury, nephritis, renal fibrosis, and diabetic nephropathy models. This review summarizes reports on renal function protection and the mechanisms of action of these ginsenosides in various renal injury models.
Mechanisms by which kidney-tonifying Chinese herbs inhibit osteoclastogenesis: Emphasis on immune cells
Authors: Jiao Yi , Wang Xing , Wang Qiong , Geng Qishun , Cao Xiaoxue , Zhang Mengxiao , Zhao Lu , Deng Tingting , Xu Yuan , Xiao Cheng
Published: February 2023

The immune system plays a crucial role in regulating osteoclast formation and function and has significance for the occurrence and development of immune-mediated bone diseases. Kidney-tonifying Chinese herbs, based on the theory of traditional Chinese medicine (TCM) to unify the kidney and strengthen the bone, have been widely used in the prevention and treatment of bone diseases. The common botanical drugs are tonifying kidney-yang and nourishing kidney-yin herbs, which are divided into two parts: one is the compound prescription of TCM, and the other is the single preparation of TCM and its active ingredients. These botanical drugs regulate osteoclastogenesis directly and indirectly by immune cells, however, we have limited information on the differences between the two botanical drugs in osteoimmunology. In this review, the mechanism by which kidney-tonifying Chinese herbs inhibiting osteoclastogenesis was investigated, emphasizing the immune response. The differences in the mechanism of action between tonifying kidney-yang herbs and nourishing kidney-yin herbs were analysed, and the therapeutic value for immune-mediated bone diseases was evaluated.
The use of remimazolam versus propofol for induction and maintenance of general anesthesia: A systematic review and meta-analysis
Authors: Ko Ching-Chung , Hung Kuo-Chuan , Illias Amina M. , Chiu Chong-Chi , Yu Chia-Hung , Lin Chien-Ming , Chen I-Wen , Sun Cheuk-Kwan
Published: February 2023

Background: The primary objective of this study was to compare the risk of hypotension, as well as the induction and recovery characteristics between remimazolam and propofol in patients receiving surgery under general anesthesia.

Methods: The Embase, Medline, Google scholar, and the Cochrane Library databases were searched from inception to March 2022 for randomized controlled trials The primary outcome was the risk of post-induction hypotension between the two agents, while the secondary outcomes included anesthetic depth, induction efficacy, time to loss of consciousness (LOC), hemodynamic profiles, time to eye opening, extubation time as well as the incidence of injection pain and postoperative nausea/vomiting (PONV).

Results: Meta-analysis of eight studies published from 2020 to 2022 involving 738 patients revealed a significantly lower risk of post-induction hypotension with the use of remimazolam compared to that with propofol [risk ratio (RR) = 0.57, 95% confidence interval (CI): 0.43 to 0.75, p < 0.0001, I2 = 12%, five studies, 564 patients]. After anesthetic induction, the anesthetic depth measured by bispectral index (BIS) was lighter in the remimazolam group than that in the propofol group (MD = 9.26, 95% confidence interval: 3.06 to 15.47, p = 0.003, I2 = 94%, five studies, 490 patients). The time to loss of consciousness was also longer in the former compared to the latter (MD = 15.49 s, 95%CI: 6.53 to 24.46, p = 0.0007, I2 = 61%, three studies, 331 patients). However, the use of remimazolam correlated with a lower risk of injection pain (RR = 0.03, 95%CI: 0.01 to 0.16, p < 0.0001, I2 = 0%, three studies, 407 patients) despite comparable efficacy of anesthetic induction (RR = 0.98, 95%CI: 0.9 to 1.06, p = 0.57, I2 = 76%, two studies, 319 patients). Our results demonstrated no difference in time to eye opening, extubation time, and risk of PONV between the two groups.

Conclusion: Remimazolam was associated with a lower risk of post-induction hypotension after anesthetic induction compared with propofol with similar recovery characteristics. Further studies are required to support our findings.

Systematic Review Registration:https://www.crd.york.ac.uk/prospero/; Identifier: CRD42022320658.
Averrhoa carambola leaves prevent dyslipidemia and oxidative stress in a rat model of poloxamer-407-induced acute hyperlipidemia
Authors: Abduh Maisa Siddiq , Saghir Sultan A. M. , Al Hroob Amir M. , Bin-Ammar Albandari , Al-Tarawni Ayat H. , Murugaiyah Vikneswaran , Mahmoud Ayman M.
Published: February 2023

Background:The star fruit [Averrhoa carambolaL (Oxalidaceae)] is traditionally used in the treatment of many ailments in many countries. It possesses several pharmacological activities, including antioxidant and anti-inflammatory effects. However, it contains the neurotoxic caramboxin and its high content of oxalic acid limits its consumption by individuals with compromised kidney function. This study assessed the anti-hyperlipidemic and antioxidant activities of different fractions of the methanolic extract ofA. carambolaleaves (MEACL).

Methods:The antioxidant activity was investigated using FRAP, and ABTS and DPPH radical-scavenging assays and the inhibitory activity toward pancreatic lipase (PL) and HMG-CoA reductase was assayedin vitro. Acute hyperlipidemia was induced by poloxamer-407 (P-407) in rats and different fractions of MEACL (n-hexane, chloroform,n-butanol, ethyl acetate (EA), water, and chloroform) were orally administered. Cholesterol and triglycerides were determined at 0, 12, 24, and 48 h and LDL-C, vLDL-C, HDL-C, lipid peroxidation (LPO) and antioxidants were assayed after 48 h. The expression of ABCA1, ABCG5, ABCG8, LDL-R, SREBP-1, and SREBP-2 and the activity of HMG-CoA reductase were assayed in the liver of P-407-administered rats treated with the EA fraction.

Results:Thein vitrodata revealed potent radical-scavenging activities of MEACL fractions with the most potent effect showed by the EA fraction that also suppressed the activities of HMG-CoA reductase and PL. In P-407-induced hyperlipidemic rats, all fractions prevented dyslipidemia as shown by the decrease in total cholesterol, triglycerides, LDL-C, vLDL-C and atherogenic index. MEACL and its fractions prevented LPO and boosted GSH, superoxide dismutase, glutathione peroxidase, and catalase in P-407-administered rats. The EA fraction showed more effective anti-hyperlipidemic and antioxidant effects than other fractions and downregulated SREBP-2 while upregulated ABCA1 and LDL-R and ameliorated LPL and HMG-CoA reductase in hyperlipidemic rats.

Conclusion:MEACL showedin vitroandin vivoantioxidant activity and the EA fraction significantly ameliorated dyslipidemia in a rat model of P-407-induced acute hyperlipidemia by modulating LPL, PL, HMG-CoA reductase, and cholesterolgenesis-related factors. Therefore, the leaves ofA. carambolarepresent a safe alternative for the star fruit particularly in kidney disease patients, and the EA is the most effective anti-hyperlipidemic and antioxidant fraction.
Stability and structural evolution of double-stranded DNA molecules under high pressures: A molecular dynamics study
Authors: Herrera-Velarde Salvador , Villanueva-Valencia José Ramón , Mendoza-Espinosa Paola , Castañeda-Priego Ramón
Published: February 2023

Conformational changes and stability of interacting double-stranded DNA chains under high hydrostatic pressure in biological systems are striking topics of importance to study several biomolecular phenomena. For example, to unravel the physiological conditions at which life might occur and to ensure the right functionality of the biochemical processes into the cell under extreme thermodynamic conditions. Furthermore, such processes could shed light on the physicochemical properties of the DNA under high confinement and how, through different mechanisms, a virus releases its genome in order to infect a cell and, therefore, to promote the process of viral replication. To achieve a few steps toward this direction, we propose an all-atomistic molecular dynamics approach in the NPT isothermal-isobaric ensemble to account for how the interplay of DNA—DNA interaction, hydrogen bonding, and the hydrostatic pressure modifies both the DNA conformational degrees of freedom and the spatial organization of the DNA chains in the available volume. We consider two interacting double-stranded DNA chains immersed in an explicit aqueous solution, i.e., water and ions. Our preliminary results highlight the role of hydrogen bonding and electrostatic interactions between DNA strands to avoid denaturation and, therefore, to provide mechanical stability for the DNA molecules. However, the structural evolution, whose kinetics depends on the relaxation of the stresses induced by the pressure, indicates that almost in all pressure conditions, the equilibrium configuration corresponds to an alignment of the two double-stranded DNA molecules along their main axis of symmetry; the rearrangement between the two approaching DNA dodecamers does not always correspond to complementary base pairs and becomes a function of the thermodynamic conditions.
Individuals with a previous symptomatic COVID-19 infection have altered heart rate and blood pressure variability during acute exercise
Authors: Chan Jillian , Senior Hailey , Homitz Jessica , Cashin Niamh , Guers John J.
Published: February 2023

Introduction: As the number of COVID-19 cases begin to diminish it is important to turn our attention to any long-term issues that may be associated with a prior infection. Cardiovascular defects have been noted following prior SARS-CoV-2 infections. However, less is known about how a previous infection alters the cardiovascular response to exercise. Further, differences may exist during exercise between previously SARS-CoV-2 positive individuals who had symptoms (symptomatic) relative to those who did not have symptoms (asymptomatic). We hypothesized that previously symptomatic (S) COVID-19 recoveries have an altered cardiovascular response to acute exercise relative to both control (CON; never infected), and previously COVID-19 positive asymptomatic (AS) individuals.

Methods: Twenty-seven subjects (CON = 9; AS = 9; S = 9) underwent 30 min of submaximal treadmill exercise. During exercise, blood pressure was recorded on the brachial artery every 5 min and 3-lead electrocardiography was measured continuously. Indirect indicators of autonomic nervous system health: heart rate variability and blood pressure variability were measured during each session. Baseline mean arterial pressure (MAP) was taken prior to exercise in seated, standing and supine positions.

Results: Blood pressure was similar (p > 0.05) amongst all three groups. There were no differences between average heart rate (HR; CON = 104 ± 4 BPM vs AS = 118 ± 6 BPM vs. S = 112 ± 3 BPM), mean arterial pressure (MAP; CON = 108 ± 4 mmHg vs. AS = 105 ± 13 mmHg vs. S = 108 ± 7 mmHg) or oxygen consumption (VO2) between groups during a bout of exercise. However, the standard deviation of the inter beat intervals of normal sinus beats, a measure of heart rate variability (HRV) (CON = 138 ± 2.8 m vs. AS = 156 ± 6 m vs. S = 77.7 ± 11 m; p < 0.05) and blood pressure variability (BPV; CON = 5.18 ± 1.1 vs. AS = 12.1 ± 0.88 mmHg vs. S = 10.2 ± 10.7 mmHg; p < 0.05) were different in our S group. Further, when HRV was assessed in the frequency domain the very low frequency was different during exercise in the S group relative to the other groups.

Discussion: Collectively, these data suggest that a previous symptomatic SARS-CoV-2 infection may alter heart rate and blood pressure regulation during exercise.
Mapping the lymphatic system across body scales and expertise domains: A report from the 2021 National Heart, Lung, and Blood Institute workshop at the Boston Lymphatic Symposium
Authors: Singhal Dhruv , Börner Katy , Chaikof Elliot L. , Detmar Michael , Hollmén Maija , Iliff Jeffrey J. , Itkin Maxim , Makinen Taija , Oliver Guillermo , Padera Timothy P. , Quardokus Ellen M. , Radtke Andrea J. , Suami Hiroo , Weber Griffin M. , Rovira Ilsa I. , Muratoglu Selen C. , Galis Zorina S.
Published: February 2023

Enhancing our understanding of lymphatic anatomy from the microscopic to the anatomical scale is essential to discern how the structure and function of the lymphatic system interacts with different tissues and organs within the body and contributes to health and disease. The knowledge of molecular aspects of the lymphatic network is fundamental to understand the mechanisms of disease progression and prevention. Recent advances in mapping components of the lymphatic system using state of the art single cell technologies, the identification of novel biomarkers, new clinical imaging efforts, and computational tools which attempt to identify connections between these diverse technologies hold the potential to catalyze new strategies to address lymphatic diseases such as lymphedema and lipedema. This manuscript summarizes current knowledge of the lymphatic system and identifies prevailing challenges and opportunities to advance the field of lymphatic research as discussed by the experts in the workshop.
Na+ and K+ transport and maturation stage ameloblast modulation
Authors: Ngu Jake , Bronckers Antonius L. J. J. , Katsura Kaitlin , Zhang Yan , Den Besten Pamela K.
Published: February 2023

Introduction: Enamel mineralization requires calcium transport into the extracellular matrix for the synthesis of hydroxyapatite (HA) crystals. Formation of HA releases protons into the matrix, which are then neutralized when ameloblasts modulate from cells with apical invaginations, the so-called ruffle-ended ameloblasts (RE), to smooth-ended ameloblasts (SE). Ameloblast modulation is associated with the translocation of the calcium exchanger Nckx4 to the apical border of RE, to remove Na+ from the enamel matrix in exchange for Ca2+ and K+. As enamel matures, Na+ and K+ in the matrix progressively decrease. However, the transporter to remove K+ from mineralizing enamel has not been identified.

Methods: Expression of K+ exchangers and channels in secretory and maturation stage of enamel organs were compared following an RNA-seq analysis. Kcnj15, which encodes the Kir4.2 inwardly rectifying K+ channel, was found to be the most upregulated internalizing K+ transporter in maturation stage of enamel organs. Kir4.2 was immunolocalized in wt, Nckx4−/−, Wdr72−/−, and fluorosed ameloblasts. Regulation of Wdr72 expression by pH was characterized in vitro and in vivo.

Results: Kir4.2 immunolocalized to the apical border of wild type (wt) mouse RE and cytosol of SE, a spatial distribution pattern shared by NCKX4. In Nckx4−/− ameloblasts, Kir4.2 also localized to the apical surface of RE and cytosol of SE. However, in fluorosed and Wdr72−/− ameloblasts, in which vesicle trafficking is disrupted, Kir4.2 remained in the cytosol. In vitro, Wdr72 was upregulated in LS8 cells cultured in medium with a pH 6.2, which is the pH of the enamel matrix underlying RE, as compared to pH 7.2 under SE.

Conclusion: Taken together these results suggest that Kir4.2 participates in K+ uptake by maturation ameloblasts, and that K+ and Na+ uptake by Kir4.2 and Nckx4, respectively, may be regulated by pH through WDR72-mediated endocytosis and membrane trafficking.
Predicting VO2max in competitive cyclists: Is the FRIEND equation the optimal choice?
Authors: Jurov Iva , Cvijić Marta , Toplišek Janez
Published: February 2023

Predicting VO2max in athletes is vital for determining endurance capacity, for performance monitoring, in clinical diagnostic procedures, and for disease management. This study aimed to assess the most suitable equation for predicting VO2max in competitive cyclists. Competitive cyclists (496 males, 84 females, Caucasian, 580 total) were included in the study from 1 January 2014 to 31 December 2019. Only subjects who were actively participating in national or international competitions and who were registered competitive cyclists and part of cycling teams at the time of the measurements were included. Subjects performed an incremental test on a cycle ergometer, and VO2max was measured as indicated by a plateau in VO2. In addition, four prediction equations (the FRIEND, Storer, Fairbarn, and Jones) were used to estimate VO2max. The predicted VO2max using the FRIEND equation was in good agreement with the measured VO2max in male and female athletes. This was reflected by a high correlation with r = 0.684 for men and r = 0.897 for women (p = 0.000), with ICC = 0.568 (95% CI 0.184, 0.752) for men and ICC = 0.881 (95% CI 0.813, 0.923) for women. Total error was 1.56 and 1.48 ml/min/kg and a minimal bias of−3.6 and −1.1 ml/min/kg (men and women, respectively). Using other equations resulted in a slight decline in agreement with the measured standard. The FRIEND equation predicted VO2max accurately with small total error, small prediction errors, and with the smallest constant error in our study cohort, indicating the potential value of using FRIEND equation also in competitive cyclists. This equation proved to have the highest accuracy both in male and female cyclists.
Magnesium application improves the morphology, nutrients uptake, photosynthetic traits, and quality of tobacco (Nicotiana tabacum L.) under cold stress
Authors: Li Jian , Muneer Muhammad Atif , Sun Aihua , Guo Qinyu , Wang Yuemin , Huang Zhenrui , Li Wenqing , Zheng Chaoyuan
Published: February 2023

Cold stress is one of the major constraints limiting the productivity of many important crops, including tobacco (Nicotiana tabacum L.) production and quality worldwide. However, the role of magnesium (Mg) nutrition in plants has been frequently overlooked, especially under cold stress, and Mg deficiency adversely affects plant growth and development. Here, we evaluated the influence of Mg under cold stress on tobacco morphology, nutrient uptake, photosynthetic and quality attributes. The tobacco plants were grown under different levels of cold stress, i.e., 8°C, 12°C, 16°C, including with a controlled temperature of 25°C, and evaluated their effects with Mg (+Mg) and without Mg (–Mg) application. Cold stress resulted in reduced plant growth. However, the +Mg alleviated the cold stress and significantly increased the plant biomass on an average of 17.8% for shoot fresh weight, 20.9% for root fresh weight, 15.7% for shoot dry weight, and 15.5% for root dry weight. Similarly, the nutrients uptake also increased on average for shoot-N (28.7%), root-N (22.4%), shoot-P (46.9%), root-P (7.2%), shoot-K (5.4%), root-K (28.9%), shoot-Mg (191.4%), root-Mg (187.2%) under cold stress with +Mg compared to –Mg. Mg application significantly boosted the photosynthetic activity (Pn 24.6%) and increased the chlorophyll contents (Chl-a (18.8%), Chl-b (25%), carotenoids (22.2%)) in the leaves under cold stress in comparison with –Mg treatment. Meanwhile, Mg application also improved the quality of tobacco, including starch and sucrose contents, on an average of 18.3% and 20.8%, respectively, compared to –Mg. The principal component analysis revealed that tobacco performance was optimum under +Mg treatment at 16°C. This study confirms that Mg application alleviates cold stress and substantially improves tobacco morphological indices, nutrient absorption, photosynthetic traits, and quality attributes. In short, the current findings suggest that Mg application may alleviate cold stress and improve tobacco growth and quality.
SSR marker based analysis for identification and of genetic diversity of non-heading Chinese cabbage varieties
Authors: Yin Jiwei , Zhao Hong , Wu Xingting , Ma Yingxue , Zhang Jingli , Li Ying , Shao Guirong , Chen Hairong , Han Ruixi , Xu Zhenjiang
Published: February 2023

As a widely cultivated vegetable in China and Southeast Asia, the breeding of non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) is widespread; more than 400 varieties have been granted new plant variety rights (PVRs) in China. Distinctness is one of the key requirements for the granting of PVRs, and molecular markers are widely used as a robust supplementary method for similar variety selection in the distinctness test. Although many genome-wide molecular markers have been developed, they have not all been well used in variety identification and tests of distinctness of non-heading Chinese cabbage. In this study, by using 423 non-heading Chinese cabbage varieties collected from different regions of China, 287 simple sequence repeat (SSR) markers were screened for polymorphisms, and 23 core markers were finally selected. The polymorphic information content (PIC) values of the 23 SSR markers ranged from 0.555 to 0.911, with an average of 0.693, and the average number of alleles per marker was 13.65. Using these 23 SSR markers, 418 out of 423 varieties could be distinguished, with a discrimination rate of 99.994%. Field tests indicated that those undistinguished varieties were very similar and could be further distinguished by a few morphological characteristics. According to the clustering results, the 423 varieties could be divided into three groups: pak-choi, caitai, and tacai. The similarity coefficient between the SSR markers and morphological characteristics was moderate (0.53), and the efficiency of variety identification was significantly improved by using a combination of SSR markers and morphological characteristics.
Employee wellbeing and cost reduction drivers of corporate social responsibility: Evidence from Congolese mining sector
Authors: Sun Huaping , Bahizire Grace Mulindwa
Published: February 2023

This research proposes an internal organizational framework that explains why firms implement corporate social responsibility (CSR). Based on the perspective of managers in the mining sector of the Democratic Republic of Congo (DRC), wellbeing of employees and cost reduction were identified as internal driving factors of CSR. This study was carried out on a sample of 103 mining, using a structural equation through LISREL. The findings of the study reveal; first, that firms' pursuit of cost reduction positively influences the implementation of CSR; second, firms are driven by CSR in order to ensure the wellbeing of their employees, fostered by compliance with labor standards in the mining sector. Therefore, CSR compliance was included as a mediating variable. Lastly, the lack of resources remains a barrier to CSR implementation. This research adds to the growing body of literature on CSR antecedents by demonstrating that in the Congolese mining sector, CSR implementation is linked to the wellbeing of employees as well as compliance to labor standards and regulations, cost reduction, and financial resources as well as human resources. This research responds to deficiency in novelty and lack of academic studies on drivers of CSR in the mining sector in Sub-Saharan regions.
An evolutionary case for polygyny to counter demographic collapse
Authors: Larsen Mads
Published: February 2023

Sex ratio theory suggests why mating practices have become dysfunctional in the West and other regions. Spain, Japan, and over 20 other nations are on course to have their populations halved by 2100, dramatically aging their citizenry. Experts and opinion makers warn that a demographic collapse cannot be absorbed by our current social order; Elon Musk proclaims this to be “the biggest threat to human civilization.” Statistics from the Nordic countries—the world’s most gender-equal region—indicate that subjective perceptions of the sex ratio in modern environments drive singledom and low reproduction. Scandinavia has the world’s highest occurrence of one-person households: 43–46%. In the past decade, the Norwegian fertility rate dropped from 2.0 to 1.5. Sex ratio studies suggest that women’s perception of there being few acceptable partners activates a polygynous mindset, which in prosperous, monogamous societies drives promiscuity to the detriment of pair-bonding. More than 6 million years of hominin evolution under promiscuous, polygynous, and monogamous regimes shaped mate preferences that evoke different cultural and behavioral responses as environments change. The Church’s imposition of lifelong monogamy contributed to the emergence of the modern world, but if this world’s gender-equal societies no longer motivate reproduction, being more open to polygyny could be worth considering as a means for increasing fertility. This article makes this case by exploring hominin mating from our last common ancestor with chimpanzees—through the genusHomo’sforager and agricultural periods—to modern Scandinavians. In the past millennium, mating practices have coevolved with the emergence of modernity, necessitating frequent cultural updates. An evolutionary analysis of Nordic works of literature illuminates the ways in which ideological narratives influence reproductive norms. The insights gleaned are considered in the context of people’s perceived sex ratio.
Is machine translation a dim technology for its users? An eye tracking study
Authors: Kasperė Ramunė , Motiejūnienė Jurgita , Patasienė Irena , Patašius Martynas , Horbačauskienė Jolita
Published: February 2023

State-of-the-art research shows that the impact of language technologies on public awareness and attitudes toward using machine translation has been changing. As machine translation acceptability is considered to be a multilayered concept, this paper employs criteria of usability, satisfaction and quality as components of acceptability measurement. The study seeks to determine whether there are any differences in the machine-translation acceptability between professional users, i.e., translators and language editors, and non-professional users, i.e., ordinary users of machine translation who use it for non-professional everyday purposes. The main research questions whether non-professional users process raw machine translation output in the same way as professional users and whether there is a difference in the processing of raw machine-translated output between users with different levels of machine-translated text acceptability are analyzed. The results of an eye tracking experiment, measuring fixation time, dwell time and glance count, indicate a difference between professional and non-professional users' cognitive processing and acceptability of machine translation output: translators and language editors spend more time overall reading the machine-translated texts, possibly because of their deeper critical awareness as well as professional attitude toward the text. In terms of acceptability overall, professional translators critically assess machine translation on all components of which confirms the findings of previous similar research. However, the study draws attention to non-professional users' lower awareness regarding machine translation quality. The study was conducted within a research project that received funding from the Research Council of Lithuania (LMTLT, agreement No S-MOD-21-2), seeking to explore and evaluate the impact on society of machine translation technological solutions.
Impact of COVID-19 on Rocky Vista University medical students’ mental health: A cross-sectional survey
Authors: Paz Dean C. , Bains Manav Singh , Zueger Morgan L. , Bandi Varasiddimounish R. , Kuo Victor Y. , Payton Mark , Ryznar Rebecca Jean
Published: February 2023

Background

The COVID-19 pandemic brought immense changes to medical school curriculums world-wide, such as the widespread adoption of virtual learning. We sought to better understand the impact on medical students’ mental health at Rocky Vista University College of Osteopathic Medicine, Parker, CO, United States. This study assessed the impact the pandemic had on anxiety and depression levels of medical students. It also assessed the impact of several domains on student mental health during the pandemic and how various sub-groups within the studied population were affected.

Methods

A cross-sectional survey was sent to students through an online anonymous google survey in May to June 2021, centered around the 7-item questionnaire used to screen for Generalized Anxiety Disorder GAD-7, 9-item questionnaire used to screen for depression PHQ-9, and self-designed questions to assess the personal impact of the pandemic. Data obtained were screened for error and analyzed with significance value of p < 0.05.

Results

A total of 152 responses were received (25.5% response rate). Of these, 64.1% identified as female, 75.8% were white, 50.3% were between ages 21–25, and 77.8% were first year medical students. During the pandemic, 79.6% of respondents felt more anxious and 65.1% felt more depressed. 67.8% of students reported feeling social isolation amidst the pandemic. Students living with friends were more likely to see a therapist for depression during the pandemic (p = 0.0169) and prescribed an antidepressant (p = 0.0394). Females and students in relationships were more likely to score higher on GAD-7 (p = 0.0194) and (p = 0.0244), respectively.

Conclusion

This study investigated the effect of the pandemic on medical students’ mental health and the need to address this issue. Results suggest that the pandemic had a negative impact on medical student’s mental health and that anxiety and depression levels worsened for pre-clinical medical students at Rocky Vista University. As such, it is imperative to incorporate additional resources to protect the well-being of medical students as they progress through their medical careers.
EFL university students’ perceptions about cross-cultural presentations via videoconferencing
Authors: Yu Li-Tang , Wu Hsiao-Ping
Published: February 2023

This study aims to examine EFL university students’ perceptions about cross-cultural videoconferencing presentations for professors in the United States. Nineteen Taiwanese English-as-foreign-language university students who studied in a night program for a bachelor’s degree attended the study. Each of them was free to choose a topic related to Taiwanese cultures and individually made a 25-min presentation plus a five-minute question-and-answer session. After the presentation, they watched recordings of their presentations, completed a five-point Likert-scale attitude survey, and wrote down their reflections. The results showed that the participants were positive about the activity. Based on the analysis of their reflections, the participants acknowledged various aspects of the cross-cultural presentations, such as the opportunities to use English meaningfully, motivation to prepare for the project, and the authentic nature of the interaction. However, the participants had concerns about their language abilities, anxiety, and limited preparation time. Finally, they mentioned the impact the presentation had on them. Pedagogical implications and suggestions for future research are provided.
Risk factors of social anxiety in patients with essential tremor
Authors: Hou Lijun , Lei Xiaoguang
Published: February 2023

Objective

To study the risk factors of social anxiety (SA) in essential tremor (ET) patients.

Methods

Motor, cognition, and SA were evaluated using the Fahn-Tolosa-Marin Tremor Rating Scale (FTMTRS), Mini-Mental State Examination (MMSE), and Liebowitz Social Anxiety Scale (LSAS) for each subject. The potential risk factors of SA in ET were analyzed using univariate analysis.

Results

A total of 80 ET patients and 85 healthy controls completed the evaluation. The LSAS evaluation showed that the prevalence of SA in the ET group was 48.8%, higher than that in controls (12.9%, P < 0.001). Female (OR = 4.959, P = 0.014), younger age (OR = 4.172, P = 0.037), and head tremor (OR = 4.707, P = 0.025) were risk factors of SA among ET patients.

Conclusion

SA is prevalent in patients with ET. Risk factors, such as female sex, age, and head tremor, should be considered for the prevention and intervention of SA in ET patients.
Unmet healthcare needs predict frailty onset in the middle-aged and older population in China: A prospective cohort analysis
Authors: Li Jun , Wu Di , Li Haomiao , Chen Jiangyun
Published: February 2023

Objectives

Older populations have a relatively high prevalence of unmet healthcare needs, which can result in poor health status. Moreover, in the coming century, frailty is expected to become one of the most serious global public health challenges. However, there is a lack of clear evidence proving an association between unmet healthcare needs and frailty. This study aimed to assess whether unmet healthcare needs predict the onset of frailty in China.

Methods

The association between frailty and unmet healthcare needs was explored by analyzing data from the China Health and Retirement Longitudinal Study (CHARLS) using random-effects logistic regression and Cox regression with time-varying exposure.

Results

At baseline, 7,719 respondents were included in the analysis. Random-effects logistic regression shows that unmet outpatient healthcare needs were associated with increased risk of both contemporaneous (adjusted OR [aOR], 1.17; 95% CI, 1.02–1.35) and lagged (aOR, 1.24; 95% CI, 1.05–1.45) frailty, as were unmet inpatient needs (contemporaneous: aOR, 1.28; 95% CI, 1.00–1.64; lagged: aOR, 1.55; 95% CI, 1.17–2.06). For respondents not classified as frail at baseline (n = 5,392), Cox regression with time-varying exposure shows significant associations of both unmet outpatient needs (adjusted HR, 1.23; 95% CI, 1.05–1.44) and unmet inpatient needs (adjusted HR, 1.48; 95% CI, 1.11–1.99) with increased risk of developing frailty.

Conclusions

Reducing unmet healthcare needs would be a valuable intervention to decrease frailty risk and promote healthy aging in middle-aged and older populations. It is urgent and essential that the equity and accessibility of the medical insurance and health delivery systems be strengthened.