bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of Fetomaternal Complications in Women of High Parity with Women of Low Parity among Saudi Women
Authors: Khan Farida Habib , Alkwai Hend Mohammed , Alshammari Reem Falah , Alenazi Fahaad , Alshammari Khalid Farhan , Sogeir Ehab Kamal Ahmed , Batool Asma , Khalid Ayesha Akbar
Published: November 2022

High parity is associated with the risk of fetomaternal complications such as gestational diabetes mellitus, hypertensive disorders, maternal anemia, preterm labor, miscarriage, postpartum hemorrhage, and perinatal and preterm mortality. The objective of the study was to compare fetomaternal complications in women of high parity with women of low parity. This involved a cohort study on a sample size of 500 women who had singleton births. Data were collected from the Maternity and Child Hospital, Ha’il, Kingdom of Saudi Arabia. Participants were classified into two groups according to parity, i.e., women of low parity and women of high parity. Socio-demographic data and pregnancy complications, such as gestational diabetes, hypertension, preeclampsia, intrauterine growth restriction, etc., were retrieved from participants’ files. Participants were followed in the postnatal ward until their discharge. The results revealed that women of high parity mostly (49%) were married before 20 years of age, less educated, obese, and were of un-booked cases. Premature babies and fetal mortality are significantly high (0.000) in this group. There is a significant difference between the two groups with respect to maternal anemia, gestational diabetes mellitus, joint pain, perineal tear, miscarriage, postpartum hemorrhage, preeclampsia, vaginal tear, and cesarean section. Determinants responsible for high parity should be identified via evidence-based medicine. Public health education programs targeting couples, weight control, nutrition, and contraception would be a cost-effective strategy for reducing the risk of possible fetomaternal complications.
Psychological Health in Intensive Care Unit Health Care Workers after the COVID-19 Pandemic
Authors: Carola Valeria , Vincenzo Cristina , Morale Chiara , Cecchi Valentina , Rocco Monica , Nicolais Giampaolo
Published: November 2022

Background: Although the COVID-19 pandemic had an impact on the general population, health care workers (HCWs) constituted one of the groups that were most adversely affected by the associated risks, owing to the significant consequences on their mental health. This study examined these psychological effects on HCWs who cared for COVID-19 patients who were admitted to the intensive care unit in an Italian hospital. Methods: Subjects were administered several self-reported questionnaires: Kessler 10 Psychological Distress Scale (K10), Perceived Stress Scale-10 (PSS), Impact of Event Scale Revised (IES-R), and Post-traumatic Growth Inventory (PTGI), as well as two open-ended questions oriented toward understanding their positive and negative emotional experience and differentiating between two phases of the emergency. Results: Overall, 45% of HCWs showed medium-to-high anxiety/depressive symptoms, whereas 60% presented with medium-to-high levels of perceived stress. In addition, 37% of subjects developed symptoms of PTSD and 50% showed post-traumatic growth in the “appreciation of life” and “new possibilities” dimensions. With regard to the open-ended questions, three themes were identified: quality of workplace relationships, sense of emotional-relational competence, and sense of clinical-technical competence. In addition, two macrocategories of responses were identified in the answers: growth and block. Conclusions: The mental health of HCWs who are involved in the front line of COVID-19 was significantly impacted by this experience, showing high levels of post-traumatic stress and anxiety and depressive symptoms more than 1 year after the emergency began. A qualitative analysis of staff experiences can be a useful guide for structuring interventions and prevention.
Psychometric Properties of the Traditional Chinese Version of the Stanford Presenteeism Scale (SPS-6) among Taiwanese Employees in Technology Companies
Authors: Ta-Thi Kim-Ngan , Chuang Kai-Jen
Published: November 2022

Little attention has been paid by employers to reduced productivity at work due to illness (presenteeism) because valid instruments to measure presenteeism are lacking. We assessed psychometric properties of the traditional Chinese version of the six-item Stanford Presenteeism Scale (CSPS-6) among Taiwanese employees in technology companies. We carried out a cross-cultural adaptation study on 196 employees. Factor analyses were used to evaluate the construct validity of the CSPS-6. Cronbach’s alpha was 0.74. The content validity of the CSPS-6 was good. Results of factor analyses confirmed the two-factor model of the CSPS-6. CSPS-6 scores were correlated with job stress (rs = −0.22, p = 0.002), the health status SF-36 (rs = 0.28 to 0.52, p < 0.0001), job satisfaction (rs = 0.41, p < 0.0001), and the presenteeism score of the Work Productivity and Activity Impairment Questionnaire: General Health (rs = −0.46, p < 0.0001). No correlations were found between presenteeism and the disability status (p = 0.19, F-value = 1.67, degrees of freedom = 2). The CSPS-6 was found to be reliable and valid in evaluating presenteeism of Taiwanese employees. Further studies should be undertaken to validate the CSPS-6 in other working populations and assess long-term effects of health problems associated with presenteeism.
Burdens of Apprentices Caused by the COVID-19 Pandemic and How They Deal with Them: A Qualitative Study Using Content Analysis One-Year Post-Breakout
Authors: Haider Katja , Humer Elke , Pieh Christoph , Plener Paul L. , Jesser Andrea
Published: November 2022

The COVID-19 pandemic resulted in a variety of burdens for apprentices and, as a result, in a need for resources to deal with them. The present study examined answers to two open-ended questions, which were part of a larger online survey about the burdens and resources during the pandemic from 1442 Austrian apprentices. Data collection took place from 29 March to 18 May 2021. The answers were analyzed with qualitative content analysis and revealed that most apprentices were burdened by pandemic-related restrictions and rules, the impairment of their social life, and in their mental health. In terms of resources, they mostly fell back on their social contacts, especially their friends. Besides their social life, many apprentices also used personal attitudes, leisure activities, and distractions to deal with their stresses. The study sheds light on some of the background reasons for the high psychological distress among apprentices. In order to better support young people in coping with stress, it is recommended to expand preventive and educational mental health measures targeted at apprentices, to facilitate access to low-threshold psychosocial services for young people, and improve financial support for the receipt of these.
Uniform Illumination Using Single-Surface Lens through Wavefront Engineering
Authors: Moaven Aria , Pahlevaninezhad Hamid , Pahlevaninezhad Masoud , Pahlevani Majid
Published: November 2022

Recent advancements in high power light-emitting diode (LED) technology have presented greenhouse industry with a more efficient and flexible alternative light source for horticulture. However, the light distribution on the plant remains a challenge that has notable implications on the plant growth. A non-uniform light distribution on the canopy with hot spots is well-known to adversely affect the yield. Here, we present a technique to engineer the light wavefront of a solid-state source using a single-surface optics, which yields a highly uniform light distribution across the plant. This technique achieves over 90% illuminance uniformity, preserved at various distances from the source, for a cone of light with an up to 120° angular range. This work aids the paradigm shift towards LEDs as a competitive light source in horticulture.
Low Temperature Conditioning Reduced the Chilling Injury by Regulating Expression of the Dehydrin Genes in Postharvest Huangguan Pear (Pyrus bretschneideri Rehd. cv. Huangguan)
Authors: Cheng Yudou , He Jingang , Feng Yunxiao , Zhao Jiangli , Guan Junfeng
Published: November 2022

‘Huangguan’ pear (Pyrus bretschneideri Rehd. cv. Huangguan) fruit is sensitive to chilling injury (CI), which exhibits peel browning spots (PBS) during cold storage. Dehydrin (DHN) is considered to be related to cold tolerance in plants, but its function in postharvest pear fruit during storage remains unclear. In this study, six PbDHNs (PbDHN1–6) genes were identified and characterized, and the PbDHN proteins were sorted into YnKn, SKn and YnSKn according to the major conserved motifs related to the number and location of K-segments, S-segments, and Y-segments. In addition, there were five cold-responsive related cis-acting elements in the promoter region of the PbDHNs. The analysis of fruit quality suggested that compared with a storage temperature at 20 °C, a storage temperature of 0 °C results in CI in ‘Huangguan’ pear fruit, while a storage temperature of 10 °C and low temperature conditioning (LTC) alleviates the CI. Moreover, gene expression results indicated that the six PbDHNs were markedly enhanced at low temperatures, especially at 0 °C. The transcripts of PbDHN1, PbDHN4, PbDHN5 and PbDHN6 were also increased in the fruit stored at 10 °C, but they were lower than that at 0 °C except PbDHN5. Compared with low temperature storage at 0 °C, LTC treatment significantly depressed the expression of PbDHN1, PbDHN2, PbDHN3, PbDHN4, and PbDHN6, while enhanced the mRNA amount of PbDHN5. In conclusion, PbDHN1, PbDHN4, PbDHN5, and PbDHN6 were closely related to the CI, and LTC lowered the CI by down-regulating the expression of PbDHN1, PbDHN4, and PbDHN6 and by up-regulating PbDHN5 in ‘Huangguan’ pear fruit.
The Challenges of Colorectal Cancer Surgery during the COVID-19 Pandemic in Romania: A Three-Year Retrospective Study
Authors: Tarta Cristi , Marian Marco , Capitanio Marco , Faur Flaviu Ionut , Duta Ciprian , Diaconescu Razvan , Oprescu-Macovei Anca Monica , Totolici Bogdan , Dobrescu Amadeus
Published: November 2022

The predictions on the influence of the SARS-CoV-2 pandemic on access to medical services in Romania predicted a 35% drop in oncological hospitalizations in 2020 compared to the previous decade, raising the hypothesis that patients with colorectal cancer can become indirect victims of the ongoing pandemic. Therefore, the aim of the current research was to observe how the COVID-19 pandemic influenced colorectal cancer surgery in Romania, to determine the level of addressability towards specialized care, to compare the cancer staging between the pandemic and pre-pandemic periods, and to observe the risk factors for disease progression. This retrospective study was spread over three years, respectively, from March 2019 to March 2022, and included a total of 198 patients with a history of colorectal cancer surgery. It was decided to perform a parallel comparison of 2019, 2020, and 2021 to observe any significant changes during the pandemic. Our clinic encountered a significant decrease in all interventions during the pandemic; although the number of CRC surgeries remained constant, the cases were more difficult, with significantly more patients presenting in emergency situations, from 31.3% in 2019 to 50.0% in 2020 and 57.1% in 2021. Thus, the number of elective surgeries decreased significantly. The proportion of TNM (tumor-node-metastasis) staging was, however, statistically significant between the pre-pandemic and pandemic period. In 2019, 13.3% of patients had stage IIa, compared with 28.8% in 2020 and 13.1% in 2021. Similarly, the proportion of very advanced colorectal cancer was higher during the pandemic period of 2020 and 2021 (12.0% in 2019 vs. 12.5% in 2020 and 25.0% in 2021), which was represented by a significantly higher proportion of patients with bowel perforation. Patients with an advanced TNM stage had a 6.28-fold increased risk of disease progression, followed by lymphovascular invasion (HR = 5.19). However, the COVID-19 pandemic, represented by admission years 2020 and 2021, did not pose a significant risk for disease progression and mortality. In-hospital mortality during the pandemic also did not change significantly. After the pandemic restrictions have been lifted, it would be advisable to conduct a widespread colorectal cancer screening campaign in order to identify any instances of the disease that went undetected during the SARS-CoV-2 pandemic.
Reasons for Turnover of Kansas Public Health Officials during the COVID-19 Pandemic
Authors: Cain Cristi , Hunt D. Charles , Armstrong Melissa , Collie-Akers Vicki L. , Ablah Elizabeth
Published: November 2022

Public health officials played a critical role in COVID-19 mitigation and response efforts. In Kansas, 51 local health department (LHD) administrators and/or local health officers left their positions due to the pandemic between 15 March 2020 and 31 August 2021. The purpose of this study was to identify factors that led to turnover of Kansas local public health officials during the COVID-19 pandemic. Those eligible to participate in this study included former LHD administrators and/or health officers who were employed at or contracted by a Kansas LHD on 15 March 2020 and resigned, retired, or were asked to resign prior to 31 August 2021. Researchers used a demographic survey, a focus group, and key informant interviews to collect data. Twelve former LHD leaders participated in this study. Four themes emerged from phenomenological analysis: politicization of public health; a perceived lack of support; stress and burnout; and the public health infrastructure not working. The findings of this study can guide the Kansas public health system to address the issues leading to turnover of leadership and prevent future turnover. Future research must explore strategies for mitigating leadership turnover and identify alternative public health structures that could be more effective.
Impact of Family-Based Economic Empowerment Intervention, Suubi+Adherence (2012–2018) on Multidimensional Poverty for Adolescents Living with HIV (ALWHIV) in Uganda
Authors: Dvalishvili Darejan , Ssewamala Fred. M. , Nabunya Proscovia , Sensoy Bahar Ozge , Kizito Samuel , Namuwonge Flavia , Namatovu Phionah
Published: November 2022

Children growing up in poverty are disproportionately affected by diseases, including HIV. In this study, we use data from Suubi+Adherence, a longitudinal randomized control trial (2012–2018) with 702 adolescents living with HIV (ALWHIV), to examine the effectiveness of a family-based multifaceted economic empowerment (EE) intervention in addressing economic instability and multidimensional poverty among ALWHIV in Southern Uganda. We constructed a Multidimensional Poverty Index of individual and household indicators, including health, assets, housing and family dynamics. We computed the proportion of multidimensionally poor children (H), estimated poverty intensity (A) and adjusted headcount ratio (M0). Using repeated measures at five-time points (baseline, years 1, 2, 3 and 4-post baseline) across two study arms: treatment (receiving the EE intervention) vs. control arm (not receiving EE), we find that both the incidence and proportion of multidimensional poverty decreased in the treatment arm vs. the control arm. Given that there is a direct link between economic instability and poor health outcomes, these findings are informative. They point to the potential for family EE interventions to decrease multidimensional poverty among vulnerable children, including ALWHIV, impacting their overall wellbeing and ability to meet their treatment needs and improve HIV care continuum outcomes.
A Comparative Field Study of Indoor Environment Quality and Work Productivity between Job Types in a Research Institute in Korea
Authors: Lee Gyu-Bae , Lee Seung-Min , Lee Seung-Eon , Jeong Jae-Weon , Lee Jong-Won
Published: November 2022

Indoor environment quality (IEQ) evaluation can help improve building satisfaction and productivity of residents. However, for more efficient analysis, it is necessary to gain a large amount of data on the differences between specific groups, such as building and resident work types. In this study, we conducted an IEQ evaluation for administrators and researchers, which are occupational groups of a research institute. The evaluation was conducted using quantitative and qualitative methods to find the relationships between IEQ satisfaction and work productivity for each job type. Our results showed that light environment and office layout were correlated with the work productivity of administrators, and light environment, office layout, thermal comfort, and sound environment were correlated with the work productivity of researchers. In addition, there was a significant difference in layout and thermal comfort items between administrators and researchers. Therefore, this study revealed significant differences in the effect of IEQ evaluation on work productivity between different occupations in a research institute.
Health and Safety Risk Mitigation among Artisanal and Small-Scale Gold Miners in Zimbabwe
Authors: Singo Josephine , Moyo Dingani , Isunju John Bosco , Bose-O’Reilly Stephan , Steckling-Muschack Nadine , Becker Jana , Mamuse Antony
Published: November 2022

Artisanal and small-scale gold mining (ASGM) is often associated with no or compromised attention to health and safety. Although headlines of fatal accidents in Zimbabwe characterise ASGM, little attention is paid to prevention strategies. This study, therefore, explores health and safety risk mitigation in ASGM in Zimbabwe to inform prevention strategies. A qualitative design was used with focus group discussions and in-depth interviews. Data were analysed using thematic analysis, coding, and descriptive statistics. Reported factors contributing to compromised health and safety included immediate causes, workplace factors, ASM related factors, and contextual factors, with interconnectedness between the causal factors. In addition, factors related to ASGM were significant. For risk mitigation, formalisation, organisation of risk reduction, behaviour change, and enforcement of prevention strategies is proposed. A multi-causal analysis is recommended for risk assessment and accident investigation. A multi-stakeholder approach could be considered for risk mitigation including community and public health interventions. However, risk mitigation has been characterised by gaps and weaknesses such as lacking ASM policy, lack of capital, poor enforcement, negative perceptions, and non-compliance. Therefore, we recommend addressing the threats associated with health and safety mitigation to ensure health and safety protection in ASGM.
Sustainability of Cocoa (Theobroma cacao) Cultivation in the Mining District of Ponce Enríquez: A Trace Metal Approach
Authors: Ramos Carolina , Ruales Jeny , Rivera-Parra José Luis , Sakakibara Masayuki , Díaz Ximena
Published: November 2022

Historically, cocoa (Theobroma cacao) has been one of Ecuador’s most important export crops. In the Ponce Enriquez district, artisanal and small gold mining (ASGM), and quarrying account for 42% of economic activities, while agriculture and livestock farming account for 30%, making the analysis of their synergy and interaction key to understanding the long term viability of the different activities. In this study, we evaluated the concentration of potentially toxic metals in different parts of the cocoa plant and fruit, in relation to mining activities within the area. Gold extraction generates pollution, including potentially toxic metals such as mercury (Hg), cadmium (Cd), arsenic (As), copper (Cu), lead (Pb) and zinc (Zn). In order to understand the mobility of these metals within the cocoa plant and fruit, the analysis was conducted separately for leaves, pod, husk and cocoa bean. Concentrations of the target metals in the different plant parts and soil were measured using ICP-MS, and the mobility and risk factors were calculated using the transfer factor (TF) and the risk ratio (HQ). The results suggest that Zn, Cd and Cu are indeed moving from the soil to cocoa leaves and beans. Furthermore, the results show that the concentrations of toxic metals in the different parts of the cocoa fruit and plant, particularly in the cocoa bean, which is used for chocolate manufacture, are not higher than those regulated by FAO food standards, as is the case of Cd, which is limited to 0.2 mg Cd/kg and in the samples analyzed does not exceed this limit. Even though the concentration of these metals does not exceed the safety standard, the presence of these potentially hazardous metals, and the fact they are absorbed by this important local crop, are worrying for the long-term sustainability of cocoa cultivation in the area. Therefore, it is fundamental to monitor the local environment, understanding the distribution of heavy metal pollution, and work with the local authorities in landscape management to minimize the exposure of crops to ASGM pollution.
Predictors of SARS-CoV-2 Infection in University Students: A Case-Control Study
Authors: Migliara Giuseppe , Renzi Erika , Baccolini Valentina , Cerri Ambrogio , Donia Pierluigi , Massimi Azzurra , Marzuillo Carolina , De Vito Corrado , Casini Leandro , Polimeni Antonella , Gaudio Eugenio , Villari Paolo ,   The Collaborating Group
Published: November 2022

Closure of Higher Education Institutions in the early phase of the SARS-CoV-2 pandemic was largely diffused. With their reopening, numerous preventive measures have been enacted, but limited evidence exists on students’ behavior that could influence their infection risk. We conducted a case-control study at the Sapienza University of Rome to identify protective and risk factors for SARS-CoV-2 infection. Students attending the campus within 48 h of SARS-CoV-2 infection were considered cases. Controls were students who come in contact with a confirmed case within the campus. Demographic features and activities carried out before positivity or contact were investigated. Multivariable logistic regression models were built to identify factors associated with SARS-CoV-2 infection, estimating adjusted odds ratios (aOR) and 95% confidence intervals (95% CI). The analysis showed an increased risk of SARS-CoV-2 infection for attending the second year or above of university (aOR 17.7, 95% CI 2.21–142.82) and participating in private parties or ceremonies (aOR 15.9, 95% CI 2.30–109.67) while living outside the family (aOR 0.08, 95% CI 0.01–0.54) and attending practical activities or libraries on campus (aOR 0.29, 95% CI 0.08–0.97) reduced the risk. Data strongly suggests that it may be safe to participate in activities organized under strict infection prevention guidelines. Tailored prevention measures might reduce the risk of infection in university students.
It Is Better for Younger Workers: The Gain Cycle between Job Crafting and Work Engagement
Authors: Topa Gabriela , Aranda-Carmena Mercedes
Published: November 2022

Job Crafting has been proposed as a new perspective, consisting in a bottom-up strategy to achieve person–job fit by emphasizing employees’ active participation and spontaneous change in job design, which is specifically adequate for older workers. Despite this fact, the cyclical influence between Work Engagement and Job Crafting over time has been less researched. We postulated that a gain cycle could be observed in the relationships between Job Crafting and its outcomes. Hence, we tested a longitudinal moderated mediation model in which Work Engagement increases over time through an increment in Job Crafting behaviors (Hypothesis 1), while this process is moderated by workers’ age (Hypothesis 2). The present study follows a three-wave design where participants (N = 126) responded to online surveys at three measurement waves, three months apart. At Time 1 and Time 3, we assessed Work Engagement, Job Crafting behavior, and demographic variables, while at Time 2 we only assessed Job Crafting. Our findings partially differ from what was expected. The findings supported that the relationship between Work Engagement at Time 1 and changes in Job Crafting behavior across time was negative and non-significant, failing to provide support for Hypothesis 1. Related to Hypothesis 2, our results are mixed. Although the interaction between changes in Job Crafting and workers’ age did not demonstrate a statistical influence on Work Engagement at Time 3, our findings suggested that the direct influence was complemented by a negative indirect effect through the longitudinal increase of Job Crafting, which mainly affects aged workers. Practical and theoretical implications are discussed.
Body Balance Analysis in the Visually Impaired Individuals Aged 18–24 Years
Authors: Urbaniak-Olejnik Marta , Loba Wawrzyniec , Stieler Olgierd , Komar Dariusz , Majewska Anna , Marcinkowska-Gapińska Anna , Hojan-Jezierska Dorota
Published: November 2022

Background: Proper body balance is maintained by both sensory, i.e., vision, hearing, vestibular, and proprioception, and motor nervous systems. Visual control facilitates balance both in a static position, as well as during movement. Visual dysfunctions can adversely affect balance and stability control. Methods: The study comprised a group of 30 individuals visually impaired from birth aged 18–24 years. The control group consisted of 50 persons in the same age range as the study group. The trial was performed as four separate tests: two on a stable surface platform, and two on an unstable surface platform. Results: No statistically significant differences were found in the posturography test on the stable platform between the study groups. When tested on an unstable ground surface, the visually impaired subjects obtained showed worse performance than the sighted persons. Statistically significant differences were observed in the majority of the analyzed results. Conclusions: Visually impaired individuals present a poorer ability to maintain balance under dynamic conditions. Tests conducted on the unstable surface platform seem to be more relevant and applicable in the diagnosis of the subjects with visual impairment, as they differentiate the subjects to a greater extent.
Phenotypic Characterization and Fine Mapping of a Major-Effect Fruit Shape QTL FS5.2 in Cucumber, Cucumis sativus L., with Near-Isogenic Line-Derived Segregating Populations
Authors: Pan Yupeng , Chen Birong , Qiao Lijun , Chen Feifan , Zhao Jianyu , Cheng Zhihui , Weng Yiqun
Published: November 2022

Cucumber (Cucumis sativus L.) fruit size/shape (FS) is an important yield and quality trait that is quantitatively inherited. Many quantitative trait loci (QTLs) for fruit size/shape have been identified, but very few have been fine-mapped or cloned. In this study, through marker-assisted foreground and background selections, we developed near-isogenic lines (NILs) for a major-effect fruit size/shape QTL FS5.2 in cucumber. Morphological and microscopic characterization of NILs suggests that the allele of fs5.2 from the semi-wild Xishuangbanna (XIS) cucumber (C. s. var. xishuangbannesis) reduces fruit elongation but promotes radial growth resulting in shorter but wider fruit, which seems to be due to reduced cell length, but increased cellular layers. Consistent with this, the NIL carrying the homozygous XIS allele (fs5.2) had lower auxin/IAA contents in both the ovary and the developing fruit. Fine genetic mapping with NIL-derived segregating populations placed FS5.2 into a 95.5 kb region with 15 predicted genes, and a homolog of the Arabidopsis CRABS CLAW (CsCRC) appeared to be the most possible candidate for FS5.2. Transcriptome profiling of NIL fruits at anthesis identified differentially expressed genes enriched in the auxin biosynthesis and signaling pathways, as well as genes involved in cell cycle, division, and cell wall processes. We conclude that the major-effect QTL FS5.2 controls cucumber fruit size/shape through regulating auxin-mediated cell division and expansion for the lateral and longitudinal fruit growth, respectively. The gibberellic acid (GA) signaling pathway also plays a role in FS5.2-mediated fruit elongation.
A 3D–Predicted Structure of the Amine Oxidase Domain of Lysyl Oxidase–Like 2
Authors: Meier Alex A. , Kuczera Krzysztof , Mure Minae
Published: November 2022

Lysyl oxidase–like 2 (LOXL2) has been recognized as an attractive drug target for anti–fibrotic and anti–tumor therapies. However, the structure–based drug design of LOXL2 has been very challenging due to the lack of structural information of the catalytically–competent LOXL2. In this study; we generated a 3D–predicted structure of the C–terminal amine oxidase domain of LOXL2 containing the lysine tyrosylquinone (LTQ) cofactor from the 2.4Å crystal structure of the Zn2+–bound precursor (lacking LTQ; PDB:5ZE3); this was achieved by molecular modeling and molecular dynamics simulation based on our solution studies of a mature LOXL2 that is inhibited by 2–hydrazinopyridine. The overall structures of the 3D–modeled mature LOXL2 and the Zn2+–bound precursor are very similar (RMSD = 1.070Å), and disulfide bonds are conserved. The major difference of the mature and the precursor LOXL2 is the secondary structure of the pentapeptide (His652–Lys653–Ala654–Ser655–Phe656) containing Lys653 (the precursor residue of the LTQ cofactor). We anticipate that this peptide is flexible in solution to accommodate the conformation that enables the LTQ cofactor formation as opposed to the β–sheet observed in 5ZE3. We discuss the active site environment surrounding LTQ and Cu2+ of the 3D–predicted structure.
Therapeutic Ultrasound Halts Progression of Chronic Kidney Disease In Vivo via the Regulation of Markers Associated with Renal Epithelial–Mesenchymal Transition and Senescence
Authors: Lin Chen-Yu , Wang Ching-Chia , Loh Jui-Zhi , Chiang Tsai-Chen , Weng Te-I , Chan Ding-Cheng , Hung Kuan-Yu , Chiang Chih-Kang , Liu Shing-Hwa
Published: November 2022

Low-intensity pulsed ultrasound (LIPUS), a therapeutic type of ultrasound, is known to enhance bone fracture repair processes and help some tissues to heal. Here, we investigated the therapeutic potential of LIPUS for the treatment of chronic kidney disease (CKD) in two CKD mouse models. CKD mice were induced using both unilateral renal ischemia/reperfusion injury (IRI) with nephrectomy and adenine administration. The left kidneys of the CKD mice were treated using LIPUS with the parameters of 3 MHz, 100 mW/cm2, and 20 min/day, based on the preliminary experiments. The mice were euthanized 14 days after IRI or 28 days after the end of adenine administration. LIPUS treatment effectively alleviated the decreases in the body weight and albumin/globulin ratio and the increases in the serum renal functional markers, fibroblast growth factor-23, renal pathological changes, and renal fibrosis in the CKD mice. The parameters for epithelial–mesenchymal transition (EMT), senescence-related signal induction, and the inhibition of α-Klotho and endogenous antioxidant enzyme protein expression in the kidneys of the CKD mice were also significantly alleviated by LIPUS. These results suggest that LIPUS treatment reduces CKD progression through the inhibition of EMT and senescence-related signals. The application of LIPUS may be an alternative non-invasive therapeutic intervention for CKD therapy.
The Stress Response of the Holothurian Central Nervous System: A Transcriptomic Analysis
Authors: Cruz-González Sebastián , Quesada-Díaz Eduardo , Miranda-Negrón Yamil , García-Rosario Raúl , Ortiz-Zuazaga Humberto , García-Arrarás José E.
Published: November 2022

Injury to the central nervous system (CNS) results in permanent damage and lack of function in most vertebrate animals, due to their limited regenerative capacities. In contrast, echinoderms can fully regenerate their radial nerve cord (RNC) following transection, with little to no scarring. Investigators have associated the regenerative capacity of some organisms to the stress response and inflammation produced by the injury. Here, we explore the gene activation profile of the stressed holothurian CNS. To do this, we performed RNA sequencing on isolated RNC explants submitted to the stress of transection and enzyme dissection and compared them with explants kept in culture for 3 days following dissection. We describe stress-associated genes, including members of heat-shock families, ubiquitin-related pathways, transposons, and apoptosis that were differentially expressed. Surprisingly, the stress response does not induce apoptosis in this system. Other genes associated with stress in other animal models, such as hero proteins and those associated with the integrated stress response, were not found to be differentially expressed either. Our results provide a new viewpoint on the stress response in the nervous system of an organism with amazing regenerative capacities. This is the first step in deciphering the molecular processes that allow echinoderms to undergo fully functional CNS regeneration, and also provides a comparative view of the stress response in other organisms.
Scoping Review on Platelets and Tumor Angiogenesis: Do We Need More Evidence or Better Analysis?
Authors: Filippelli Arianna , Del Gaudio Cinzia , Simonis Vittoria , Ciccone Valerio , Spini Andrea , Donnini Sandra
Published: November 2022

Platelets are an active component of the tumor microenvironment (TME), involved in the regulation of multiple tumor processes, including angiogenesis. They are generated rich in angiogenic factors in their granules to actively participate in the hemostatic process by megakaryocytes and further enriched in angiogenic factors by all components of the tumor microenvironment to control the angiogenic process because of their preferential relationship with the endothelial component of vessels. In recent decades, the literature has reported a great deal of evidence on the role of platelets in tumor angiogenesis; however, it is unclear whether the number or mean volume of platelets and/or their content and localization in TME may have clinical relevance in the choice and management of therapy for the cancer patient. In this scoping review, we collected and critically reviewed the scientific evidence supporting a close relationship between platelets, cancer, and angiogenesis. The aim of this work was to define the landscape of platelet-activated angiogenesis in cancer progression and analyze what and how much evidence is present in the last 20 years in the literature at both the preclinical and clinical levels, to answer whether platelets could be a useful determinant for analyzing tumor angiogenesis. In conclusion, this scoping review indicates that there is much evidence, both preclinical and clinical, but in the preclinical context, studies demonstrate the direct involvement of platelets in tumor angiogenesis; in the clinical context the evidence is indirect, though strong, and the indication of how and to what extent platelet content contributes to tumor angiogenesis is lacking. So, do we need more evidence or better analysis? More molecular and quali-quantitative data is needed to translate the results obtained in preclinical studies into the clinical setting. This information about platelets, if correlated with tumor type and its biology, including tumor vasculature, type of angiogenesis, and patient characteristics (age, sex, comorbidities, drug treatments for chronic diseases) could be an important pa- rameter for correlating platelet biology to angiogenesis, for personalizing cancer therapy, and for clinical prognosis.
Genomic Determinants Potentially Associated with Clinical Manifestations of Human-Pathogenic Tick-Borne Flaviviruses
Authors: Bondaryuk Artem N. , Kulakova Nina V. , Potapova Ulyana V. , Belykh Olga I. , Yudinceva Anzhelika V. , Bukin Yurij S.
Published: November 2022

The tick-borne flavivirus group contains at least five species that are pathogenic to humans, three of which induce encephalitis (tick-borne encephalitis virus, louping-ill virus, Powassan virus) and another two species induce hemorrhagic fever (Omsk hemorrhagic fever virus, Kyasanur Forest disease virus). To date, the molecular mechanisms responsible for these strikingly different clinical forms are not completely understood. Using a bioinformatic approach, we performed the analysis of each amino acid (aa) position in the alignment of 323 polyprotein sequences to calculate the fixation index (Fst) per site and find the regions (determinants) where sequences belonging to two designated groups were most different. Our algorithm revealed 36 potential determinants (Fst ranges from 0.91 to 1.0) located in all viral proteins except a capsid protein. In an envelope (E) protein, most of the determinants were located on the virion surface regions (domains II and III) and one (absolutely specific site 457) was located in the transmembrane region. Another 100% specific determinant site (E63D) with Fst = 1.0 was located in the central hydrophilic domain of the NS2b, which mediates NS3 protease activity. The NS5 protein contains the largest number of determinants (14) and two of them are absolutely specific (T226S, E290D) and are located near the RNA binding site 219 (methyltransferase domain) and the extension structure. We assume that even if not absolutely, highly specific sites, together with absolutely specific ones (Fst = 1.0) can play a supporting role in cell and tissue tropism determination.
Genetic Deletion of Mmp9 Does Not Reduce Airway Inflammation and Structural Lung Damage in Mice with Cystic Fibrosis-like Lung Disease
Authors: Wagner Claudius , Balázs Anita , Schatterny Jolanthe , Zhou-Suckow Zhe , Duerr Julia , Schultz Carsten , Mall Marcus A.
Published: November 2022

Elevated levels of matrix metalloprotease 9 (MMP-9) and neutrophil elastase (NE) are associated with bronchiectasis and lung function decline in patients with cystic fibrosis (CF). MMP-9 is a potent extracellular matrix-degrading enzyme which is activated by NE and has been implicated in structural lung damage in CF. However, the role of MMP-9 in the in vivo pathogenesis of CF lung disease is not well understood. Therefore, we used β-epithelial Na+ channel-overexpressing transgenic (βENaC-Tg) mice as a model of CF-like lung disease and determined the effect of genetic deletion of Mmp9 (Mmp9-/-) on key aspects of the pulmonary phenotype. We found that MMP-9 levels were elevated in the lungs of βENaC-Tg mice compared with wild-type littermates. Deletion of Mmp9 had no effect on spontaneous mortality, inflammatory markers in bronchoalveolar lavage, goblet cell metaplasia, mucus hypersecretion and emphysema-like structural lung damage, while it partially reduced mucus obstruction in βENaC-Tg mice. Further, lack of Mmp9 had no effect on increased inspiratory capacity and increased lung compliance in βENaC-Tg mice, whereas both lung function parameters were improved with genetic deletion of NE. We conclude that MMP-9 does not play a major role in the in vivo pathogenesis of CF-like lung disease in mice.
Two-Photon Excitation Spectra of Various Fluorescent Proteins within a Broad Excitation Range
Authors: Leben Ruth , Lindquist Randall L. , Hauser Anja E. , Niesner Raluca , Rakhymzhan Asylkhan
Published: November 2022

Two-photon excitation fluorescence laser-scanning microscopy is the preferred method for studying dynamic processes in living organ models or even in living organisms. Thanks to near-infrared and infrared excitation, it is possible to penetrate deep into the tissue, reaching areas of interest relevant to life sciences and biomedicine. In those imaging experiments, two-photon excitation spectra are needed to select the optimal laser wavelength to excite as many fluorophores as possible simultaneously in the sample under consideration. The more fluorophores that can be excited, and the more cell populations that can be studied, the better access to their arrangement and interaction can be reached in complex systems such as immunological organs. However, for many fluorophores, the two-photon excitation properties are poorly predicted from the single-photon spectra and are not yet available, in the literature or databases. Here, we present the broad excitation range (760 nm to 1300 nm) of photon-flux-normalized two-photon spectra of several fluorescent proteins in their cellular environment. This includes the following fluorescent proteins spanning from the cyan to the infrared part of the spectrum: mCerulean3, mTurquoise2, mT-Sapphire, Clover, mKusabiraOrange2, mOrange2, LSS-mOrange, mRuby2, mBeRFP, mCardinal, iRFP670, NirFP, and iRFP720.
PET-Derived Radiomics and Artificial Intelligence in Breast Cancer: A Systematic Review
Authors: Urso Luca , Manco Luigi , Castello Angelo , Evangelista Laura , Guidi Gabriele , Castellani Massimo , Florimonte Luigia , Cittanti Corrado , Turra Alessandro , Panareo Stefano
Published: November 2022

Breast cancer (BC) is a heterogeneous malignancy that still represents the second cause of cancer-related death among women worldwide. Due to the heterogeneity of BC, the correct identification of valuable biomarkers able to predict tumor biology and the best treatment approaches are still far from clear. Although molecular imaging with positron emission tomography/computed tomography (PET/CT) has improved the characterization of BC, these methods are not free from drawbacks. In recent years, radiomics and artificial intelligence (AI) have been playing an important role in the detection of several features normally unseen by the human eye in medical images. The present review provides a summary of the current status of radiomics and AI in different clinical settings of BC. A systematic search of PubMed, Web of Science and Scopus was conducted, including all articles published in English that explored radiomics and AI analyses of PET/CT images in BC. Several studies have demonstrated the potential role of such new features for the staging and prognosis as well as the assessment of biological characteristics. Radiomics and AI features appear to be promising in different clinical settings of BC, although larger prospective trials are needed to confirm and to standardize this evidence.
Correction: Mahmoudi et al. Solvent-Induced Formation of Novel Ni(II) Complexes Derived from Bis-Thiosemicarbazone Ligand: An Insight from Experimental and Theoretical Investigations. Int. J. Mol. Sci. 2021, 22, 5337
Authors: Mahmoudi Ghodrat , Babashkina Maria G. , Maniukiewicz Waldemar , Afkhami Farhad Akbari , Nunna Bharath Babu , Zubkov Fedor I. , Ptaszek Aleksandra L. , Szczepanik Dariusz W. , Mitoraj Mariusz P. , Safin Damir A.
Published: November 2022

We received a complaint from the Université Catholique de Louvain [...]
Epigenetic Mechanisms of Tree Responses to Climatic Changes
Authors: Kurpisz Barbara , Pawłowski Tomasz Andrzej
Published: November 2022

Forest trees are complex perennial organisms that are adapted to the local environment in the results of prevailing climate conditions in population history. Because they lead a sedentary lifestyle, plants are exposed to various environmental stimuli, such as changes which can lead to the rapid adjustment or failure of their defence mechanisms. As forests play a key role in environment homeostasis and are the source of many products, it is crucial to estimate the role of forest trees’ plasticity mechanisms in the face of the climate change. Fast epigenetic adjustment is the basis for surviving climate fluctuations, however the question is whether this mechanism will be also efficient if climate fluctuations increase. Epigenetic modifications enable rapid reactions to the inducing stimulus by establishing chromatin patterns and manipulating gene expression without affecting the DNA itself. This work aimed to gather information about the epigenetic mechanisms of tree responses to changing environmental conditions, in order to summarise what is known so far and emphasize the significance of the discussed issue. Applying this knowledge in the future to study the interactions between climate change and gene regulation at the levels of plant development could generate answers to questions about the limitations of plasticity of plant adaptation to changing environment. We still know very little about how organisms, especially trees, cope with climate change and we believe that this overview will encourage researchers to fill this gap in the knowledge, and that results will be applied in improving defensive capacity of this ecologically and economically important species.
Investigation on Purification of Saturated LiNO3 Solution Using Titanium Phosphate Ion Exchanger: Kinetics Study
Authors: Maslova Marina , Ivanenko Vladimir , Evstropova Polina , Mudruk Natalia , Gerasimova Lidia
Published: November 2022

Lithium compounds are of high interest to many industries. The presence of undesirable impurities in Li precursors leads to uncontrolled change in the functional properties of final compounds. Therefore, the development of reliable methods for lithium salt purification is considered a key factor for their application in various industries. This work focuses on the application of a titanium phosphate ion exchanger (Li-TiOP) toward Cu2+, Co2+, Mn2+, Ni2+, and Cr3+ ions in the purification of a saturated LiNO3 solution. The sorption kinetics of the selected ions, considering external and internal mass transfer, as well as chemical interaction, were deeply studied. The kinetic study showed that the values of intraparticle diffusion rate and effective diffusion coefficients for the studied ions decreased in the following order: Cr(III) ˃ Cu(II) Mn(II) ˃ Co(II) ˃ Ni(II). For all the selected ions, chemical interaction was described with a pseudo-second-order reaction model. The sorption kinetics were controlled by the size of the solvated metal ion, its effective charge, the electronic structure of the adsorbed ion, and the interaction with the functional groups of the sorbent. Due to fast kinetics, the high degree of removal of trace quantities of the impurities this material gives it consideration as a promising sorbent for the deep purification of lithium salts.
Proprotein Convertase Subtilisin/Kexin 6 in Cardiovascular Biology and Disease
Authors: Wu Qingyu , Chen Shenghan
Published: November 2022

Proprotein convertase subtilisin/kexin 6 (PCSK6) is a secreted serine protease expressed in most major organs, where it cleaves a wide range of growth factors, signaling molecules, peptide hormones, proteolytic enzymes, and adhesion proteins. Studies in Pcsk6-deficient mice have demonstrated the importance of Pcsk6 in embryonic development, body axis specification, ovarian function, and extracellular matrix remodeling in articular cartilage. In the cardiovascular system, PCSK6 acts as a key modulator in heart formation, lipoprotein metabolism, body fluid homeostasis, cardiac repair, and vascular remodeling. To date, dysregulated PCSK6 expression or function has been implicated in major cardiovascular diseases, including atrial septal defects, hypertension, atherosclerosis, myocardial infarction, and cardiac aging. In this review, we describe biochemical characteristics and posttranslational modifications of PCSK6. Moreover, we discuss the role of PCSK6 and related molecular mechanisms in cardiovascular biology and disease.