bluuebunny/crossref_metadata_2025 huggingface dataset:
Pasta Enriched with Dried and Powdered Leek: Physicochemical Properties and Changes during Cooking
Authors: Biernacka Beata , Dziki Dariusz , Gawlik-Dziki Urszula
Published: July 2022

Food enrichment is commonly applied to increase the pro-health and dietary value of final products. This study aimed to evaluate how green leek powder (GL) added to semolina influenced the physicochemical, sensory, and cooking characteristics of the pasta. The pasta was prepared by partially substituting semolina with 0, 1, 2, 3, 4, and 5 g/100 g of GL. Then, the pasta samples were assessed for color, cooking properties, sensory features, mechanical properties, total phenolic content (TPC), and antioxidant activity (AA). The results indicated that GL significantly decreased the lightness and increased the yellowness of cooked pasta. The total color difference between the control pasta and enriched pasta samples ranged from 2.54 to 6.89 and 5.60 to 11.06 (for uncooked and cooked pasta, respectively). The addition of GL also caused an increase in the optimal cooking time and cooking loss. Moreover, a significant increase in stretching force was observed in cooked pasta. Sensory evaluation revealed that substitution of semolina with up to 2 g/100 g GL did not affect the smell, taste, and color of pasta. Most importantly, GL-enriched pasta was characterized by higher TPC and higher AA compared to the control samples. In summary, our results recommend partial replacement of semolina by GL (up to 3 g/100 g) in pasta production.
Therapeutic Perspectives of CD26 Inhibitors in Imune-Mediated Diseases
Authors: Hu Xiaopeng , Wang Xisheng , Xue Xingkui
Published: July 2022

The enzymatic activity of CD26/DPP4 (dipeptidyl peptidase 4/DPP4) is highlighted in multiple studies to play a vital role in glucose metabolism by cleaving and inactivating the incretins glucagon-like peptide-1 (GLP) and gastric inhibitory protein (GIP). A large number of studies demonstrate that CD26 also plays an integral role in the immune system, particularly in T cell activation. CD26 is extensively expressed in immune cells, such as T cells, B cells, NK cells, dendritic cells, and macrophages. The enzymatic activity of CD26 cleaves and regulates numerous chomokines and cytokines. CD26 inhibitors have been widely used for the treatment of diabetes mellitus, while it is still under investigation as a therapy for immune-mediated diseases. In addition, CD26’s involvement in cancer immunology was also described. The review aims to summarize the therapeutic effects of CD26 inhibitors on immune-mediated diseases, as well as the mechanisms that underpin them.
Development of Small-Molecule Fluorescent Probes Targeting Enzymes
Authors: Li Yuan-Xiang , Xie Dong-Tai , Yang Ya-Xi , Chen Zhao , Guo Wu-Yingzheng , Yang Wen-Chao
Published: July 2022

As biological catalysts, enzymes are vital in controlling numerous metabolic reactions. The regulation of enzymes in living cells and the amount present are indicators of the metabolic status of cell, whether in normal condition or disease. The small-molecule fluorescent probes are of interest because of their high sensitivity and selectivity, as well as their potential for automated detection. Fluorescent probes have been useful in targeting particular enzymes of interest such as proteases and caspases. However, it is difficult to develop an ideal fluorescent probe for versatile purposes. In the future, the design and synthesis of enzyme-targeting fluorescent probes will focus more on improving the selectivity, sensitivity, penetration ability and to couple the fluorescent probes with other available imaging molecules/technologies.
Anti-Aging Effect of Momordica charantia L. on d-Galactose-Induced Subacute Aging in Mice by Activating PI3K/AKT Signaling Pathway
Authors: Wang Dongxue , Wang Enhui , Li Ying , Teng Yaran , Li Hui , Jiao Lili , Wu Wei
Published: July 2022

Anti-aging is a challenging and necessary research topic. Momordica charantia L. is a common edible medicinal plant that has various pharmacological activities and is often employed in daily health care. However, its anti-aging effect on mice and the underlying mechanism thereof remain unclear. Our current study mainly focused on the effect of Momordica charantia L. on d-galactose-induced subacute aging in mice and explored the underlying mechanism. UHPLC-Q-Exactive Orbitrap MS was applied to qualitatively analyze the chemical components of Momordica charantia L. ethanol extract (MCE). A subacute aging mice model induced by d-galactose (d-gal) was established to investigate the anti-aging effect and potential mechanism of MCE. The learning and memory ability of aging mice was evaluated using behavioral tests. The biochemical parameters, including antioxidant enzyme activity and the accumulation of lipid peroxides in serum, were measured to explore the effect of MCE on the redox imbalance caused by aging. Pathological changes in the hippocampus were observed using hematoxylin and eosin (H&E) staining, and the levels of aging-related proteins in the PI3K/AKT signaling pathway were assessed using Western blotting. The experimental results demonstrated that a total of 14 triterpenoids were simultaneously identified in MCE. The behavioral assessments results showed that MCE can improve the learning and memory ability of subacute mice. The biochemical parameters determination results showed that MCE can improve the activity of antioxidant enzymes and decrease the accumulation of lipid peroxides in aging mice significantly. Furthermore, aging and injury in the hippocampus were ameliorated. Mechanistically, the results showed a significant upregulation in the protein expression of P-PI3K/PI3K and P-AKT/AKT (p < 0.01), as well as a significant reduction in cleaved caspase-3/caspase-3, Bax and P-mTOR/mTOR (p < 0.01). Our results confirm that MCE could restore the antioxidant status and improve cognitive impairment in aging mice, inhibit d-gal-induced apoptosis by regulating the PI3K/AKT signaling pathway, and rescue the impaired autophagy caused by mTOR overexpression, thereby exerting an anti-aging effect.
Advances in Fibrin-Based Materials in Wound Repair: A Review
Authors: Bayer Ilker S.
Published: July 2022

The first bioprocess that occurs in response to wounding is the deterrence of local hemorrhage. This is accomplished by platelet aggregation and initiation of the hemostasis cascade. The resulting blood clot immediately enables the cessation of bleeding and then functions as a provisional matrix for wound healing, which begins a few days after injury. Here, fibrinogen and fibrin fibers are the key players, because they literally serve as scaffolds for tissue regeneration and promote the migration of cells, as well as the ingrowth of tissues. Fibrin is also an important modulator of healing and a host defense system against microbes that effectively maintains incoming leukocytes and acts as reservoir for growth factors. This review presents recent advances in the understanding and applications of fibrin and fibrin-fiber-incorporated biomedical materials applied to wound healing and subsequent tissue repair. It also discusses how fibrin-based materials function through several wound healing stages including physical barrier formation, the entrapment of bacteria, drug and cell delivery, and eventual degradation. Pure fibrin is not mechanically strong and stable enough to act as a singular wound repair material. To alleviate this problem, this paper will demonstrate recent advances in the modification of fibrin with next-generation materials exhibiting enhanced stability and medical efficacy, along with a detailed look at the mechanical properties of fibrin and fibrin-laden materials. Specifically, fibrin-based nanocomposites and their role in wound repair, sustained drug release, cell delivery to wound sites, skin reconstruction, and biomedical applications of drug-loaded fibrin-based materials will be demonstrated and discussed.
Novel Sterically Crowded and Conformationally Constrained α-Aminophosphonates with a Near-Neutral pKa as Highly Accurate 31P NMR pH Probes. Application to Subtle pH Gradients Determination in Dictyostelium discoideum Cells
Authors: Delehedde Caroline , Culcasi Marcel , Ricquebourg Emilie , Cassien Mathieu , Siri Didier , Blaive Bruno , Pietri Sylvia , Thétiot-Laurent Sophie
Published: July 2022

In order to discover new 31P NMR markers for probing subtle pH changes (<0.2 pH unit) in biological environments, fifteen new conformationally constrained or sterically hindered α-aminophosphonates derived from diethyl(2-methylpyrrolidin-2-yl)phosphonate were synthesized and tested for their pH reporting and cytotoxic properties in vitro. All compounds showed near-neutral pKas (ranging 6.28–6.97), chemical shifts not overlapping those of phosphorus metabolites, and spectroscopic sensitivities (i.e., chemical shifts variation Δδab between the acidic and basic forms) ranging from 9.2–10.7 ppm, being fourfold larger than conventional endogenous markers such as inorganic phosphate. X-ray crystallographic studies combined with predictive empirical relationships and ab initio calculations addressed the inductive and stereochemical effects of substituents linked to the protonated amine function. Satisfactory correlations were established between pKas and both the 2D structure and pyramidalization at phosphorus, showing that steric crowding around the phosphorus is crucial for modulating Δδab. Finally, the hit 31P NMR pH probe 1b bearing an unsubstituted 1,3,2-dioxaphosphorinane ring, which is moderately lipophilic, nontoxic on A549 and NHLF cells, and showing pKa = 6.45 with Δδab = 10.64 ppm, allowed the first clear-cut evidence of trans-sarcolemmal pH gradients in normoxic Dictyostelium discoideum cells with an accuracy of <0.05 pH units.
Drug-Loaded Polymeric Particulated Systems for Ophthalmic Drugs Release
Authors: Mihailovici Ruxandra , Croitoriu Alexandra , Nedeff Florin , Nedeff Valentin , Ochiuz Lacramioara , Vasincu Decebal , Popa Ovidiu , Agop Maricel , Moraru Andreea , Costin Danut , Costuleanu Marcel , Verestiuc Liliana
Published: July 2022

Drug delivery to the anterior or posterior segments of the eye is a major challenge due to the protection barriers and removal mechanisms associated with the unique anatomical and physiological nature of the ocular system. The paper presents the preparation and characterization of drug-loaded polymeric particulated systems based on pre-emulsion coated with biodegradable polymers. Low molecular weight biopolymers (chitosan, sodium hyaluronate and heparin sodium) were selected due to their ability to attach polymer chains to the surface of the growing system. The particulated systems with dimensions of 190–270 nm and a zeta potential varying from −37 mV to +24 mV depending on the biopolymer charges have been obtained. Current studies show that particles release drugs (dexamethasone/pilocarpine/bevacizumab) in a safe and effective manner, maintaining therapeutic concentration for a longer period of time. An extensive modeling study was performed in order to evaluate the drug release profile from the prepared systems. In a multifractal paradigm of motion, nonlinear behaviors of a drug delivery system are analyzed in the fractal theory of motion, in order to correlate the drug structure with polymer. Then, the functionality of a SL(2R) type “hidden symmetry” implies, through a Riccati type gauge, different “synchronization modes” (period doubling, damped oscillations, quasi-periodicity and intermittency) during the drug release process. Among these, a special mode of Kink type, better reflects the empirical data. The fractal study indicated more complex interactions between the angiogenesis inhibitor Bevacizumab and polymeric structure.
In Silico Drug Repurposing of FDA-Approved Drugs Highlighting Promacta as a Potential Inhibitor of H7N9 Influenza Virus
Authors: Mtambo Sphamandla E. , Kumalo Hezekiel M.
Published: July 2022

Influenza virus infections continue to be a significant and recurrent public health problem. Although vaccine efficacy varies, regular immunisation is the most effective method for suppressing the influenza virus. Antiviral drugs are available for influenza, although two of the four FDA-approved antiviral treatments have resulted in significant drug resistance. Therefore, new treatments are being sought to reduce the burden of flu-related illness. The time-consuming development of treatments for new and re-emerging diseases such as influenza and the high failure rate are increasing concerns. In this context, we used an in silico-based drug repurposing method to repurpose FDA-approved drugs as potential therapies against the H7N9 virus. To find potential inhibitors, a total of 2568 drugs were screened. Promacta, tucatinib, and lurasidone were identified as promising hits in the DrugBank database. According to the calculations of MM-GBSA, tucatinib (−54.11 kcal/mol) and Promacta (−56.20 kcal/mol) occupied the active site of neuraminidase with a higher binding affinity than the standard drug peramivir (−49.09 kcal/mol). Molecular dynamics (MD) simulation studies showed that the C-α atom backbones of the complexes of tucatinib and Promacta neuraminidase were stable throughout the simulation period. According to ADME analysis, the hit compounds have a high gastrointestinal absorption (GI) and do not exhibit properties that allow them to cross the blood–brain barrier (BBB). According to the in silico toxicity prediction, Promacta is not cardiotoxic, while lurasidone and tucatinib show only weak inhibition. Therefore, we propose to test these compounds experimentally against the influenza H7N9 virus. The investigation and validation of these potential H7N9 inhibitors would be beneficial in order to bring these compounds into clinical settings.
LMWP (S3-3) from the Larvae of Musca domestica Alleviate D-IBS by Adjusting the Gut Microbiota
Authors: Peng Siyuan , Ling Xiwen , Rui Wenjing , Jin Xiaobao , Chu Fujiang
Published: July 2022

Diarrhea-based Irritable Bowel Syndrome (D-IBS) and diarrhea are both associated with ecological imbalance of the gut microbiota. Low Molecular Weight Peptides (LMWP) from the larvae of Musca domestica have been shown to be effective in the treatment of diarrhea and regulation of gut microbiota. Meanwhile, the single polypeptide S3-3 was successfully isolated and identified from LMWP in our previous studies. It remains unclear exactly whether and how LMWP (S3-3) alleviate D-IBS through regulating gut microbiota. We evaluated the gut microbiota and pharmacology to determine the regulation of gut microbiota structure and the alleviating effect on D-IBS through LMWP (S3-3). The rates of loose stools, abdominal withdrawal reflex (AWR) and intestinal tract motility results revealed that LMWP (S3-3) from the larvae of Musca domestica had a regulating effect against diarrhea, visceral hypersensitivity and gastrointestinal (GI) dysfunction in D-IBS model mice. Additionally, 16S rRNA gene sequencing was utilized to examine the gut microbiota, which suggests that LMWP induce structural changes in the gut microbiota and alter the levels of the following gut microbiota: Bacteroidetes, Proteobacteria and Verrucomicrobia. LMWP putatively functioned through regulating 5-HT, SERT, 5-HT2AR, 5-HT3AR and 5-HT4R according to the results of ELISA, qRT-PCR and IHC. The findings of this study will contribute to further understanding how LMWP (S3-3) attenuate the effects of D-IBS on diarrhea, visceral hypersensitivity and GI dysfunction.
A Study on Attention Attracting Elements of 360-Degree Videos Based on VR Eye-Tracking System
Authors: Choi Haram , Nam Sanghun
Published: July 2022

In 360-degree virtual reality (VR) videos, users possess increased freedom in terms of gaze movement. As a result, the users’ attention may not move according to the narrative intended by the director and miss out on important parts of the narrative of the 360-degree video. Therefore, it is necessary to study a directing technique that can attract user attention in 360-degree VR videos. In this study, we analyzed the directing elements that can attract users’ attention in a 360-degree VR video and developed a 360 VR eye-tracking system to investigate the effect of the attention-attracting elements on the user. Elements that can attract user attention were classified into five categories: object movement, hand gesture, GUI insertion, camera movement, and gaze angle variation. We developed a 360 VR eye-tracking system to analyze whether five attention-attracting elements influence the user’s attention. Based on the eye tracking system, an experiment was conducted to analyze whether the user’s attention moves according to the five attention-attracting elements. Based on the experimental results, it can be seen that ‘hand gesture’ attracted the second most attention shift of the subjects, and ‘GUI insertion’ induced the smallest shift of attention of the subjects.
Hollow Bioelectrodes Based on Buckypaper Assembly. Application to the Electroenzymatic Reduction of O2
Authors: Buzzetti Paulo Henrique M. , Berezovska Anastasiia , Nedellec Yannig , Cosnier Serge
Published: July 2022

A new concept of hollow electrode based on the assembly of two buckypapers creating a microcavity which contains a biocatalyst is described. To illustrate this innovative concept, hollow bioelectrodes containing 0.16–4 mg bilirubin oxidase in a microcavity were fabricated and applied to electroenzymatic reduction of O2 in aqueous solution. For hemin-modified buckypaper, the bioelectrode shows a direct electron transfer between multi-walled carbon nanotubes and bilirubin oxidase with an onset potential of 0.77 V vs. RHE. The hollow bioelectrodes showed good storage stability in solution with an electroenzymatic activity of 30 and 11% of its initial activity after 3 and 6 months, respectively. The co-entrapment of bilirubin oxidase and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) in the microcavity leads to a bioelectrode exhibiting mediated electron transfer. After 23 h of intermittent operation, 5.66 × 10−4 mol of O2 were electroreduced (turnover number of 19,245), the loss of catalytic current being only 54% after 7 days.
Copper Zinc Sulfide (CuZnS) Quantum Dot-Decorated (NiCo)–S/Conductive Carbon Matrix as the Cathode for Li–S Batteries
Authors: Artchuea Thanphisit , Srikhaow Assadawoot , Sriprachuabwong Chakrit , Tuantranont Adisorn , Tang I-Ming , Pon-On Weeraphat
Published: July 2022

Sulfur composites consisting of electrochemical reactive catalysts/conductive materials are investigated for use in lithium–sulfur (Li–S) batteries (LSBs). In this paper, we report the synthesis, physicochemical and electrochemical properties of CuZnS quantum dots (CZSQDs) decorated with nickel–cobalt–sulfide ((NiCo)–S)) mixed with reduced graphene oxide (rGO)/oxidized carbon nanotube (oxdCNT) (rGO/oxdCNT) ((NiCo)–S@rGO/oxdCNT) composites. These composites are for the purpose of being the sulfur host cathode in Li–S batteries. The as-prepared composites showed a porous structure with the CZSQDs being uniformly found on the surface of the rGO/oxdCNT, which had a specific surface area of 26.54 m2/g. Electrochemical studies indicated that the (NiCo)–S@rGO/oxdCNT cells forming the cathode exhibited a maximum capacity of 1154.96 mAhg−1 with the initial discharge at 0.1 C. The smaller size of the CZSQDs (~10 nm) had a positive effect on the CZSQDs@(NiCo)–S@rGO/oxdCNT composites in that they had a higher initial discharge capacity of 1344.18 mAhg−1 at 0.1 C with the Coulombic efficiency being maintained at almost 97.62% during cycling. This latter property is approximately 1.16 times more compared to the absence of the Cu–Zn–S QD loading. This study shows that the CuZnS quantum dots decorated with a (NiCo)–S@rGO/oxdCNT supporting matrix-based sulfur cathode have the potential to improve the performance of future lithium–sulfur batteries.
Temperature Dependence of Electron Leakage Current in InGaN Blue Light-Emitting Diode Structures
Authors: Onwukaeme Chibuzo , Lee Bohae , Ryu Han-Youl
Published: July 2022

We investigated the temperature dependence of the electron leakage current in the AlGaN electron-blocking layer (EBL) of an InGaN/GaN blue light-emitting diode (LED) structure at temperatures between 20 and 100 °C. The percentage of electron leakage current was experimentally determined by fitting the measured external quantum efficiency of an LED using the ABC recombination model. The electron leakage current decreased significantly as the temperature increased from 20 to 100 °C. The experiment obtained temperature-dependent electron leakage current was also found to agree well with the simulation results. This counter-intuitive temperature dependence of the electron leakage current resulted from an increase in potential barrier for electrons with increasing temperature due to the increased ionized acceptor concentration in the EBL with temperature. Moreover, the results obtained for the temperature-dependent electron leakage were consistent with the thermionic emission model. The results of the temperature dependence reported here are expected to provide insight into the thermal droop of GaN-based LEDs.
Molecular Dynamic Simulation of Defective Graphene Nanoribbons for Tension and Vibration
Authors: Mao Jia-Jia , Liu Shuang , Li Lili , Chen Jie
Published: July 2022

As deformation and defects are inevitable during the manufacture and service of graphene resonators, comprehensive molecular dynamic (MD) simulations are performed to investigate the vibrational properties of the defective single-layer graphene sheets (SLGSs) during tension. Perfect SLGSs, SLGSs with single vacancy, SLGSs with low-concentration vacancies, and SLGSs with high-concentration vacancies are considered, respectively. The frequencies of the perfect and defective SLGSs at different stretching stages are investigated in detail. The effects of different external forces are also taken into account to study the vibration properties of the defective SLGSs. Results show that the perfect and defective SLGSs both successively perform four stages, i.e., the elastic stage, the yield stage, the hardening stage, and the fracture stage during stretching, and the elastic properties of the SLGSs are insensitive to the vacancy defects, while the ultimate strain is noticeably reduced by the vacancies. The single vacancy has no effect on the vibration properties of SLGS, while the frequency decreases with the increasing vacancy concentration for SLGS at the elastic stage. The frequency of yielded SLGS with a certain vacancy concentration is almost constant even with a varying external force.
Pearson’s Principle-Inspired Robust 2D Amorphous Ni-Fe-Co Ternary Hydroxides on Carbon Textile for High-Performance Electrocatalytic Water Splitting
Authors: Hu Rong , Jiang Huiyu , Xian Jinglin , Mi Shiyun , Wei Liyun , Fang Guangyu , Guo Jiayue , Xu Siqi , Liu Ziyang , Jin Huanyu , Yu Huimin , Wan Jun
Published: July 2022

Layered double hydroxide (LDH) is widely used in electrocatalytic water splitting due to its good structural tunability, high intrinsic activity, and mild synthesis conditions, especially for flexible fiber-based catalysts. However, the poor stability of the interface between LDH and flexible carbon textile prepared by hydrothermal and electrodeposition methods greatly affects its active area and cyclic stability during deformation. Here, we report a salt-template-assisted method for the growth of two-dimensional (2D) amorphous ternary LDH based on dip-rolling technology. The robust and high-dimensional structure constructed by salt-template and fiber could achieve a carbon textile-based water splitting catalyst with high loading, strong catalytic activity, and good stability. The prepared 2D NiFeCo-LDH/CF electrode showed overpotentials of 220 mV and 151 mV in oxygen evolution and hydrogen evolution reactions, respectively, and simultaneously had no significant performance decrease after 100 consecutive bendings. This work provides a new strategy for efficiently designing robust, high-performance LDH on flexible fibers, which may have great potential in commercial applications.
Polarization-Induced Phase Transitions in Ultra-Thin InGaN-Based Double Quantum Wells
Authors: Łepkowski Sławomir P. , Anwar Abdur Rehman
Published: July 2022

We investigate the phase transitions and the properties of the topological insulator in InGaN/GaN and InN/InGaN double quantum wells grown along the [0001] direction. We apply a realistic model based on the nonlinear theory of elasticity and piezoelectricity and the eight-band k·p method with relativistic and nonrelativistic linear-wave-vector terms. In this approach, the effective spin–orbit interaction in InN is negative, which represents the worst-case scenario for obtaining the topological insulator in InGaN-based structures. Despite this rigorous assumption, we demonstrate that the topological insulator can occur in InGaN/GaN and InN/InGaN double quantum wells when the widths of individual quantum wells are two and three monolayers (MLs), and three and three MLs. In these structures, when the interwell barrier is sufficiently thin, we can observe the topological phase transition from the normal insulator to the topological insulator via the Weyl semimetal, and the nontopological phase transition from the topological insulator to the nonlocal topological semimetal. We find that in InGaN/GaN double quantum wells, the bulk energy gap in the topological insulator phase is much smaller for the structures with both quantum well widths of 3 MLs than in the case when the quantum well widths are two and three MLs, whereas in InN/InGaN double quantum wells, the opposite is true. In InN/InGaN structures with both quantum wells being three MLs and a two ML interwell barrier, the bulk energy gap for the topological insulator can reach about 1.2 meV. We also show that the topological insulator phase rapidly deteriorates with increasing width of the interwell barrier due to a decrease in the bulk energy gap and reduction in the window of In content between the normal insulator and the nonlocal topological semimetal. For InN/InGaN double quantum wells with the width of the interwell barrier above five or six MLs, the topological insulator phase does not appear. In these structures, we find two novel phase transitions, namely the nontopological phase transition from the normal insulator to the nonlocal normal semimetal and the topological phase transition from the nonlocal normal semimetal to the nonlocal topological semimetal via the buried Weyl semimetal. These results can guide future investigations towards achieving a topological insulator in InGaN-based nanostructures.
New Cryogels Based on Poly(vinyl alcohol) and a Copolymacrolactone System: I-Synthesis and Characterization
Authors: Crețu Bianca-Elena-Beatrice , Nita Loredana Elena , Șerban Alexandru-Mihail , Rusu Alina Gabriela , Doroftei Florica , Chiriac Aurica P.
Published: July 2022

Physical cryogels were obtained using the successive freeze–thaw technique of poly(vinyl alcohol) (PVA)/poly(ethylene brassylate-co-squaric acid) (PEBSA) solutions. The cryogel systems were prepared by using two different molecular weights of PVA and PEBSA with three different ratios between the ethylene brassylate (EB) and squaric acid (SA) comonomers. The presence of interactions, the thermal properties and the morphology were investigated using Fourier Transform Infrared Spectroscopy (FT-IR), thermogravimetry (TGA and DTG) and scanning electron microscopy (SEM), respectively. The influence of the composition on the degree of swelling in a physiological environment was demonstrated. The study highlighted improvements in terms of new network flexibility due to the intermolecular chains interactions brought by the introduction of PEBSA in the cryogel structure. We also concluded that the presence of PEBSA in the PVA/PEBSA cryogel network improved the loading capacity of the new system with specific hydrophobic agents, for example essential oils, which (due to their antimicrobial character) can lead to the use of new systems obtained for various applications.
Investigation of Combinatorial WO3-MoO3 Mixed Layers by Spectroscopic Ellipsometry Using Different Optical Models
Authors: Fried Miklos , Bogar Renato , Takacs Daniel , Labadi Zoltan , Horvath Zsolt Endre , Zolnai Zsolt
Published: July 2022

Reactive (Ar-O2 plasma) magnetron sputtered WO3-MoO3 (nanometer scaled) mixed layers were investigated and mapped by Spectroscopic Ellipsometry (SE). The W- and Mo-targets were placed separately, and 30 × 30 cm glass substrates were slowly moved under the two (W and Mo) separated targets. We used different (oscillator- and Effective Medium Approximation, EMA-based) optical models to obtain the thickness and composition maps of the sample layer relatively quickly and in a cost-effective and contactless way. In addition, we used Rutherford Backscattering Spectrometry to check the SE results. Herein, we compare the “goodness” of different optical models depending upon the sample preparation conditions, for instance, the speed and cycle number of the substrate motion. Finally, we can choose between appropriate optical models (2-Tauc-Lorentz oscillator model vs. the Bruggeman Effective Medium Approximation, BEMA) depending on the process parameters. If one has more than one “molecular layer” in the “sublayers”, BEMA can be used. If one has an atomic mixture, the multiple oscillator model is better (more precise) for this type of layer structure.
Insight into a Fenton-like Reaction Using Nanodiamond Based Relaxometry
Authors: Padamati Sandeep Kumar , Vedelaar Thea Annie , Perona Martínez Felipe , Nusantara Anggrek Citra , Schirhagl Romana
Published: July 2022

Copper has several biological functions, but also some toxicity, as it can act as a catalyst for oxidative damage to tissues. This is especially relevant in the presence of H2O2, a by-product of oxygen metabolism. In this study, the reactions of copper with H2O2 have been investigated with spectroscopic techniques. These results were complemented by a new quantum sensing technique (relaxometry), which allows nanoscale magnetic resonance measurements at room temperature, and at nanomolar concentrations. For this purpose, we used fluorescent nanodiamonds (FNDs) containing ensembles of specific defects called nitrogen-vacancy (NV) centers. More specifically, we performed so-called T1 measurements. We use this method to provide real-time measurements of copper during a Fenton-like reaction. Unlike with other chemical fluorescent probes, we can determine both the increase and decrease in copper formed in real time.
Optical and Photocatalytic Properties of Br-Doped BiOCl Nanosheets with Rich Oxygen Vacancies and Dominating {001} Facets
Authors: Zhang Qian , Nie Wuyang , Hou Tian , Shen Hao , Li Qiang , Guan Chongshang , Duan Libing , Zhao Xiaoru
Published: July 2022

Crystal facet engineering and nonmetal doping are regarded as effective strategies for improving the separation of charge carriers and photocatalytic activity of semiconductor photocatalysts. In this paper, we developed a facial method for fabricating oxygen-deficient Br-doped BiOCl nanosheets with dominating {001} facets through a traditional hydrothermal reaction and explored the impact of the Br doping and specific facets on carrier separation and photocatalytic performance. The morphologies, structures, and optical and photocatalytic properties of the obtained products were characterized systematically. The BiOCl samples prepared by the hydrothermal reaction exhibited square-like shapes with dominating {001} facets. Photodeposition results indicated that photoinduced electrons preferred to transfer to {001} facets because of the strong internal static electric fields in BiOCl nanosheets with dominating {001} facets. Br doping not only contributed to the formation of impurity energy levels that could promote light absorption but introduced a large number of surface oxygen vacancies (VO) in BiOCl photocatalysts, which was beneficial for photocatalytic performance. Moreover, the photocatalytic activities of these products under visible light were tested by degradation of rhodamine B (RhB). Because of the synergistic effect of the dominating {001} facets, Br doping, and rich VO, oxygen-deficient Br-doped BiOCl nanosheets exhibited improved carrier separation, visible light absorption, and photocatalytic efficiency.
Magnesium Intake, C-Reactive Protein, and Muscle Mass in Adolescents
Authors: Dong Yutong , Chen Li , Gutin Bernard , Huang Ying , Dong Yanbin , Zhu Haidong
Published: July 2022

Background: Adult studies have suggested that magnesium intake may regulate C-reactive protein (CRP) and muscle mass, known risk factors for cardiometabolic diseases. Given the large deficiencies in magnesium intake in adolescents, we aimed to investigate sex and race differences in dietary magnesium intake and test the hypothesis that lower magnesium intake is associated with higher CRP and lower muscle mass. Methods: A total of 766 black and white adolescents, 14 to 18 years old (51% black; 50% female) were previously recruited. Diet was assessed with four to seven independent 24-h recalls. Body composition was measured by dual-energy X-ray absorptiometry. High-sensitivity CRP (hs-CRP), leptin, resistin, and adiponectin were measured using fasting blood samples by ELISA. Results: There were sex and race differences in the daily consumption of magnesium. The average daily magnesium intakes were 200.66 ± 7.09 mg and 205.03 ± 7.05 mg for males and females, respectively, far below the recommended amounts of 410 mg for males and 360 mg for females. White subjects (217.95 ± 6.81 mg/day) consumed more than black subjects (187.75 ± 6.92 mg/day). Almost none of the adolescents met the recommendations. Adjusted multiple linear regressions revealed that lower magnesium intake was associated with higher hs-CRP and lower fat-free mass (FFM) (p-values < 0.05). Higher hs-CRP was associated with lower FFM. Moreover, an interaction between magnesium intake and hs-CRP on FFM was identified (p-value < 0.05). Lower magnesium intake amplified the inverse relationships between hs-CRP and FFM (p-values < 0.05). Conclusion: Magnesium consumption in our adolescents was far below daily recommended levels with male and black subjects consuming less than female and white subjects. Lower magnesium intake was associated with higher CRP and lower muscle mass. Low magnesium intake may also augment the inverse relationship between CRP and FFM.
The Effects of Fatty Acids on Inflammatory Bowel Disease: A Two-Sample Mendelian Randomization Study
Authors: He Jian , Luo Xiaobei , Xin Hongjie , Lai Qianwei , Zhou Yuanping , Bai Yang
Published: July 2022

Inflammatory Bowel Disease (IBD) is a severe relapsing inflammation of the gastrointestinal tract. The association between fatty acids (FAs) and IBD is controversial and it remains unclear whether there is a causal relationship between them. Mendelian randomization (MR) analysis was province/state for affiliations from the same country performed to clarify the causality. Eligible single nucleotide polymorphisms were selected as instrumental variables from six Genome-wide association studies, involving 114,999 individuals in UK Biobank. The summary-level data on IBD, including Crohn’s disease (CD) and ulcerative colitis (UC), were obtained from the International Inflammatory Bowel Disease Genetics Consortium with 20,883 and 27,432 individuals involved. The primary inverse variance weighted (IVW) method as well as other supplementary analysis ones were adopted to evaluate the causal relationship between diverse FAs and IBD. The tests for heterogeneity and pleiotropy, and Leave-one-out analysis were adopted to verify the stability of the results. Omega-3 FA was found to have a causal effect on UC instead of CD. For each Standard Deviation increase in Omega-3 FA genetic levels, the risk of ulcerative colitis was found to be reduced by 39.9% by the IVW method (p = 1.766 × 10−4), by 57.8% by the MR Egger (p = 1.11 × 10−2), by 51.5% by the Weighted median estimator (p = 7.706 × 10−4), by 39% by the Maximum likelihood estimation (p = 3.262 × 10−4), and by 54.5% by the penalized weighted median estimator (p = 1.628 × 10−4). No causal relationship was found between other FAs (including total FA, saturated FA, polyunsaturated FA, monounsaturated FA and omega-6 FA) and IBD. The pleiotropic test and Leave-one-out analysis both proved the validity and reliability of these MR analyses. Omega-3 FA was observed to have a protective effect against UC, providing a new perspective on the investigation of the associations between FAs and IBD.
Secondary Metabolites in the Dendrobium heterocarpum Methanolic Extract and Their Impacts on Viability and Lipid Storage of 3T3-L1 Pre-Adipocytes
Authors: Warinhomhoun Sakan , Khine Hnin Ei Ei , Sritularak Boonchoo , Likhitwitayawuid Kittisak , Miyamoto Tomofumi , Tanaka Chiaki , Punsawad Chuchard , Punpreuk Yanyong , Sungthong Rungroch , Chaotham Chatchai
Published: July 2022

Although many natural products have proven their potential to regulate obesity through the modulation of adipocyte biology, none of them has yet been approved for clinical use in obesity therapy. This work aims to isolate valuable secondary metabolites from an orchid species (Dendrobium heterocarpum) and evaluate their possible roles in the growth and differentiation of 3T3-L1 pre-adipocytes. Six compounds were isolated from the orchid’s methanolic extracts and identified as amoenylin (1), methyl 3-(4-hydroxyphenyl) propionate (2), 3,4-dihydroxy-5,4’-dimethoxybibenzyl (3), dendrocandin B (4), dendrofalconerol A (5), and syringaresinol (6). Among these phytochemicals, compounds 2, 3, and 6 exhibited lower effects on the viability of 3T3-L1 cells, offering non-cytotoxic concentrations of ≲10 µM. Compared to others tested, compound 3 was responsible for the maximum reduction of lipid storage in 3T3-L1 adipocytes (IC50 = 6.30 ± 0.10 µM). A set of protein expression studies unveiled that compound 3 at non-cytotoxic doses could suppress the expression of some key transcription factors in adipocyte differentiation (i.e., PPARγ and C/EBPα). Furthermore, this compound could deactivate some proteins involved in the MAPK pathways (i.e., JNK, ERK, and p38). Our findings prove that D. heterocarpum is a promising source to explore bioactive molecules capable of modulating adipocytic growth and development, which can potentially be assessed and innovated further as pharmaceutical products to defeat obesity.
Whey Derivatives and Galactooligosaccharides Stimulate the Wound Healing and the Function of Human Keratinocytes through the NF-kB and FOXO-1 Signaling Pathways
Authors: Bergandi Loredana , Flutto Tania , Valentini Sabina , Thedy Laura , Pramotton Rita , Zenato Simona , Silvagno Francesca
Published: July 2022

Skin repair requires the activation of keratinocytes and is mediated by controlled inflammation and cell migration and proliferation, ending with the regeneration of well-differentiated cell layers. Whey derivatives contain galactooligosaccharides (GOS), which have potential beneficial effects on wound healing due to their activity as toll-like receptor ligands, although their direct nonprebiotic effects in the skin have not yet been described. In this study, we investigated the effects of different whey-derived products and purified GOS on a human keratinocyte cell line. We found that the inflammatory cytokine interleukin-8 (IL-8) was upregulated by nuclear factor kappa B (NF-kB) signaling triggered by whey derivatives and GOS and that wound healing was accelerated by promoting cell migration and the loss of E-cadherin in the absence of epithelial–mesenchymal transition. Interestingly, the treatments enhanced the mitochondrial function in association with the translocation of the Forkhead Box O1 (FOXO-1) transcription factor. Finally, we detected the increased expression of the differentiation markers induced by GOS and whey derivatives. All together, our results show that GOS-containing products can promote wound closure and skin health by direct activity on keratinocyte functions. Among the preparations tested, the fermented compound produced by autochthonous microorganisms was the most active in modulating keratinocyte activity, supporting the biological value of whey derivatives for health.
Dietary Patterns, Food Insecurity, and Their Relationships with Food Sources and Social Determinants in Two Small Island Developing States
Authors: Bhagtani Divya , Augustus Eden , Haynes Emily , Iese Viliamu , Brown Catherine R. , Fesaitu Jioje , Hambleton Ian , Badrie Neela , Kroll Florian , Saint-Ville Arlette , Samuels Thelma Alafia , Forouhi Nita G. , Benjamin-Neelon Sara E. , Unwin Nigel ,   on behalf of the CFaH Team
Published: July 2022

Small Island Developing States (SIDS) have high burdens of nutrition-related chronic diseases. This has been associated with lack of access to adequate and affordable nutritious foods and increasing reliance on imported foods. Our aim in this study was to investigate dietary patterns and food insecurity and assess their associations with socio-demographic characteristics and food sources. We recruited individuals aged 15 years and above from rural and urban areas in Fiji (n = 186) and St. Vincent and the Grenadines (SVG) (n = 147). Data collection included a 24 h diet recall, food source questionnaire and the Food Insecurity Experience Scale. We conducted latent class analysis to identify dietary patterns, and multivariable regression to investigate independent associations with dietary patterns. Three dietary patterns were identified: (1) low pulses, and milk and milk products, (2) intermediate pulses, and milk and milk products and (3) most diverse. In both SIDS, dietary pattern 3 was associated with older age, regularly sourcing food from supermarkets and borrowing, exchanging, bartering or gifting (BEB). Prevalence of food insecurity was not statistically different across dietary patterns. In both SIDS, food insecurity was higher in those regularly sourcing food from small shops, and in SVG, lower in those regularly using BEB. These results complement previous findings and provide a basis for further investigation into the determinants of dietary patterns, dietary diversity and food insecurity in these settings.
Serum Osteoprotegerin Is an Independent Marker of Left Ventricular Hypertrophy, Systolic and Diastolic Dysfunction of the Left Ventricle and the Presence of Pericardial Fluid in Chronic Kidney Disease Patients
Authors: Romejko Katarzyna , Rymarz Aleksandra , Szamotulska Katarzyna , Bartoszewicz Zbigniew , Niemczyk Stanisław
Published: July 2022

Background: Osteoprotegerin (OPG) is a molecule which belongs to the tumor necrosis factor receptor superfamily. OPG concentration is elevated in patients with left ventricle hypertrophy, heart failure and acute myocardial infarction. OPG concentrations rise in chronic kidney disease (CKD). The aim of this study was to investigate the association between OPG concentrations and cardiovascular complications, such as left ventricle hypertrophy, systolic and diastolic dysfunction of left ventricle and dysfunction of right ventricle in chronic kidney disease patients not treated with dialysis. The relation between OPG and the amount of pericardial fluid was also examined. Methods: One hundred and one men with CKD stage 3–5 not treated with dialysis were included in the study. Overhydration, body fat mass and lean body mass were measured using bioimpedance spectroscopy (BIS). Echocardiography was performed to evaluate the amount of pericardial fluid and to measure the thickness of the interventricular septum (IVS), systolic and diastolic function of left ventricle, as well as systolic function of right ventricle. Results: We observed a significant positive association between OPG and the thickness of the interventricular septum, the size of the left atrium (LA) and the presence of pericardial fluid. A negative relationship was observed between OPG and ejection fraction (EF). Conclusions: Our results suggest that OPG can be an independent marker of left ventricular hypertrophy, systolic and diastolic dysfunction of left ventricle and the presence of pericardial fluid in chronic kidney disease patients.
Alteration in Gut Microbiota Associated with Zinc Deficiency in School-Age Children
Authors: Chen Xiaohui , Jiang Yu , Wang Zhuo , Chen Youhai , Tang Shihua , Wang Shuyue , Su Li , Huang Xiaodan , Long Danfeng , Wang Liang , Guo Wei , Zhang Ying
Published: July 2022

Zinc deficiency could lead to a dynamic variation in gut microbial composition and function in animals. However, how zinc deficiency affects the gut microbiome in school-age children remains unclear. The purpose of this study was to profile the dynamic shifts in the gut microbiome of school-age children with zinc deficiency, and to determine whether such shifts are associated with dietary intake. A dietary survey, anthropometric measurements, and serum tests were performed on 177 school-age children, and 67 children were selected to explore the gut microbial community using amplicon sequencing. School-age children suffered from poor dietary diversity and insufficient food and nutrient intake, and 32% of them were zinc deficient. The inflammatory cytokines significantly increased in the zinc deficiency (ZD) group compared to that in the control (CK) group (p < 0.05). There was no difference in beta diversity, while the Shannon index was much higher in the ZD group (p < 0.05). At the genus level, Coprobacter, Acetivibrio, Paraprevotella, and Clostridium_XI were more abundant in the ZD group (p < 0.05). A functional predictive analysis showed that the metabolism of xenobiotics by cytochrome P450 was significantly depleted in the ZD group (p < 0.05). In conclusion, gut microbial diversity was affected by zinc deficiency with some specific bacteria highlighted in the ZD group, which may be used as biomarkers for further clinical diagnosis of zinc deficiency.
Weight Loss Management and Lifestyle Changes during COVID-19 Lockdown: A Matched Italian Cohort Study
Authors: De Amicis Ramona , Foppiani Andrea , Galasso Letizia , Montaruli Angela , Roveda Eliana , Esposito Fabio , Battezzati Alberto , Bertoli Simona , Leone Alessandro
Published: July 2022

During the COVID-19 lockdown, lifestyle deterioration had a negative impact on weight, and yet no study has focused on patients already undergoing dietary therapy. We performed a cohort study among adults to evaluate the effect of lockdown on weight loss programs, and we investigated changes in eating habits and chronotype. We matched confined cases with non-confined cases among individuals who followed the same diet in 2017–2019. At baseline, all patients underwent a clinical examination and completed questionnaires on lifestyle. At follow-up, patients of the confined group were interviewed by a web call, and questionnaires were re-evaluated. We recruited 61 patients. The confined sample was mainly composed of middle-aged (52 (43,58) years) females (46 (75%)) with overweight (27 (44%)) or obesity (24 (39%)) and a moderate physical activity level (48 (81%)). Body weight at follow-up was significantly higher (1.1 (95% CI: 0.14, 2.1) kg) in the confined group adjusting for all matching variables. Adherence to the Mediterranean diet and eating behavior generally improved. Concerning chronotype, patients differentiated from Neither-types to Evening- and Morning-types. A well-monitored dietary therapy maintains weight loss during lockdown. Improvement in eating habits was observed; however, a shift of the circadian typology occurred.