bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Early Fluid Balance on Long-Term Mortality in Critically Ill Surgical Patients: A Retrospective Cohort Study in Central Taiwan
Authors: Wu Chieh-Liang , Pai Kai-Chih , Wong Li-Ting , Wang Min-Shian , Chao Wen-Cheng
Published: October 2021

Fluid balance is an essential issue in critical care; however, the impact of early fluid balance on the long-term mortality in critically ill surgical patients remains unknown. This study aimed to address the impact of day 1–3 and day 4–7 fluid balance on the long-term mortality in critically ill surgical patients. We enrolled patients who were admitted to surgical intensive care units (ICUs) during 2015–2019 at a tertiary hospital in central Taiwan and retrieved date-of-death from the Taiwanese nationwide death registration profile. We used a Log-rank test and a multivariable Cox proportional hazards regression model to determine the independent mortality impact of early fluid balance. A total of 6978 patients were included for analyses (mean age: 60.9 ± 15.9 years; 63.9% of them were men). In-hospital mortality, 90-day mortality, 1-year and overall mortality was 10.3%, 15.8%, 23.8% and 31.7%, respectively. In a multivariable Cox proportional hazard regression model adjusted for relevant covariates, we found that positive cumulative day 4–7 fluid balance was independently associated with long-term mortality (aHR 1.083, 95% CI 1.062–1.105), and a similar trend was found on day 1–3 fluid balance, although to a lesser extent (aHR 1.027, 95% CI 1.011–1.043). In conclusion, the fluid balance in the first week of ICU stay, particularly day 4–7 fluid balance, may affect the long-term outcome in critically ill surgical patients.
Are Sarcopenia and Myosteatosis in Elderly Patients with Pelvic Ring Injury Related to Mortality, Physical Functioning and Quality of Life?
Authors: Banierink Hester , Bombach Julia J. C. , ten Duis Kaj , IJpma Frank F. A. , Heineman Erik , van Helden Sven H. , Nijveldt Robert J. , Viddeleer Alain R. , Reininga Inge H. F.
Published: October 2021

The purpose of this study was to evaluate the prevalence of sarcopenia and/or myosteatosis in elderly patients with pelvic ring injuries and their influence on mortality, patient-perceived physical functioning and quality of life (QoL). A multicenter retrospective cohort study was conducted including elderly patients aged ≥ 65 treated for a pelvic ring injury. Cross-sectional computed tomography (CT) muscle measurements were obtained to determine the presence of sarcopenia and/or myosteatosis. Kaplan–Meier analysis was used for survival analysis, and Cox proportional hazards regression analysis was used to determine risk factors for mortality. Patient-reported outcome measures for physical functioning (SMFA) and QoL (EQ-5D) were used. Multivariable linear regression analyses were used to determine the effect of sarcopenia and myosteatosis on patient-perceived physical functioning and QoL. Data to determine sarcopenia and myosteatosis were available for 199 patients, with a mean follow-up of 2.4 ± 2.2 years: 66 patients (33%) were diagnosed with sarcopenia and 65 (32%) with myosteatosis, while 30 of them (15%) had both. Mortality rates in patients at 1 and 3 years without sarcopenia and myosteatosis were 13% and 21%, compared to 11% and 36% in patients with sarcopenia, 17% and 31% in patients with myosteatosis and 27% and 43% in patients with both. Higher age at the time of injury and a higher Charlson Comorbidity Index (CCI) were independent risk factors for mortality. Patient-reported mental and emotional problems were significantly increased in patients with sarcopenia.
Behavioural Difficulties in Children and Adolescents with Mental Disorders under Extreme Situations
Authors: Gonzalez-Domenech Pablo , Romero-Béjar José , Gutierrez-Rojas Luis , Jimenez-Fernandez Sara , Diaz-Atienza Francisco
Published: October 2021

In 2020, the Governments of many countries maintained different levels of confinement of the population due to the pandemic that produced the COVID-19. There are few studies published on the psychological impact in the child and adolescent population diagnosed with mental disorders, especially during the home confinement stage. Explanatory models based on socio-demographic and clinical variables provide an approximation to level changes in different dimensions of behavioural difficulties. A categorical-response logistic ordinal regression model, based on a cross-sectional study with 139 children and adolescents diagnosed with mental disorders is performed for each dimension under analysis. Most of the socio-demographic and clinical explanatory variables considered (24 of 26) were significant at population level for at least one of the four dimensions of behavioural difficulties (15 response variables) under analysis. Odds-ratios were interpreted to identify risk or protective factors increasing or decreasing severity in the response variable. This analysis provides useful information, making it possible to more readily anticipate critical situations due to extreme events, such as a confinement, in this population.
Differences between Mycobacterium chimaera and tuberculosis Using Ocular Multimodal Imaging: A Systematic Review
Authors: Zweifel Sandrine Anne , Foa Nastasia , Wiest Maximilian Robert Justus , Carnevali Adriano , Zaluska-Ogryzek Katarzyna , Rejdak Robert , Toro Mario Damiano
Published: October 2021

Due to their non-specific diagnostic patterns of ocular infection, differential diagnosis between Mycobacterium (M.) chimaera and tuberculosis can be challenging. In both disorders, ocular manifestation can be the first sign of a systemic infection, and a delayed diagnosis might reduce the response to treatment leading to negative outcomes. Thus, it becomes imperative to distinguish chorioretinal lesions associated with M. chimaera, from lesions due to M. tuberculosis and other infectious disorders. To date, multimodal non-invasive imaging modalities that include ultra-wide field fundus photography, fluorescein and indocyanine green angiography, optical coherence tomography and optical coherence tomography angiography, facilitate in vivo examination of retinal and choroidal tissues, enabling early diagnosis, monitoring treatment response, and relapse detection. This approach is crucial to differentiate between active and inactive ocular disease, and guides clinicians in their decisional-tree during the patients’ follow-up. In this review, we summarized and compared the available literature on multimodal imaging data of M. chimaera infection and tuberculosis, emphasizing similarities and differences in imaging patterns between these two entities and highlighting the relevance of multimodal imaging in the management of the infections.
Association between Pulpal-Periapical Pathology and Autoimmune Diseases: A Systematic Review
Authors: Guerrero-Gironés Julia , Ros-Valverde Antonio , Pecci-Lloret María Pilar , Rodríguez-Lozano Francisco Javier , Pecci-Lloret Miguel Ramón
Published: October 2021

Several studies have linked apical periodontitis and systemic diseases. The aim of this study is to present a systematic review of the available literature investigating whether there is an association between pulpal-periapical pathology and autoimmune disease. The review was conducted following the PRISMA statement. A literature search was performed in five databases. Studies involving patients with pulpal-periapical pathology and autoimmune diseases were included in the review. Based on the PICO model, the research question aimed to assess whether there is an increased risk of developing pulpal-periapical pathology in patients with autoimmune disease. Article selection, data extraction, and quality assessment were performed using an adapted version of the STROBE guidelines. A total of seven studies were included in our review. The types of articles were five case-control and two cross-sectional studies. Periapical pathologies were associated to three autoimmune diseases (diabetes mellitus I, rheumatoid arthritis, and inflammatory bowel disease). Among the included studies, four show a low risk of bias, while three present a moderate risk. There could be an association between apical periodontitis and autoimmune diseases, although most studies report statistically non-significant associations.
Emerging Therapies for Advanced Cholangiocarcinoma: An Updated Literature Review
Authors: Vignone Anthony , Biancaniello Francesca , Casadio Marco , Pesci Ludovica , Cardinale Vincenzo , Ridola Lorenzo , Alvaro Domenico
Published: October 2021

Cholangiocarcinoma is a group of malignancies with poor prognosis. Treatments for the management of advanced-stage cholangiocarcinoma are limited, and the 5-year survival rate is estimated to be approximately 5–15%, considering all tumor stages. There is a significant unmet need for effective new treatment approaches. The present review is provided with the aim of summarizing the current evidence and future perspectives concerning new therapeutic strategies for cholangiocarcinoma. The role of targeted therapies and immunotherapies is currently investigational in cholangiocarcinoma. These therapeutic options might improve survival outcomes, as shown by the promising results of several clinical trials illustrated in the present review. The co-presence of driver mutations and markers of susceptibility to immunotherapy may lead to rational combination strategies and clinical trial development. A better understanding of immunologically based therapeutic weapons is needed, which will lead to a form of a precision medicine strategy capable of alleviating the clinical aggressiveness and to improve the prognosis of cholangiocarcinoma.
The Influence of Deterioration of Kidney Function on the Diagnostic Power of Laboratory Parameters Used in the Prognostic Classification of AL Amyloidosis
Authors: Czyżewska Emilia , Ciepiela Olga
Published: October 2021

There is a possibility that renal dysfunction may potentially reduce the diagnostic power of the laboratory parameters Tn, NT-proBNP and sFLC levels, used in the current prognostic classification of AL amyloidosis and the diagnosis of heart involvement by amyloid. In this study, the impact of lowering the eGFR value on the usefulness of these parameters in the prognosis and diagnosis of the presence of amyloid in the myocardium was assessed in a group of 71 patients with newly diagnosed primary amyloidosis. The assessment of diagnostic power of laboratory parameters was performed on the entire study group, and in the ranges of eGFR ≥ 60 and < 60 mL/min/1.73 m2. It has been proven that, with a decrease in the eGFR value, the concentrations of NT-proBNP and the κ uninvolved light chains increase significantly (p < 0.001). To assess the diagnostic power of laboratory parameters used in the diagnosis of myocardial involvement in patients with AL amyloidosis, an ROC analysis was performed. The highest values of AUC were obtained for the NT-proBNP concentration (AUC = 0.906). The lowest values of the AUC and Youden’s index were obtained for the dFLC values (AUC = 0.723), and involved κ FLC concentration (AUC = 0.613). For all compared parameters, the smallest values of the AUC were obtained for eGFR (<60 mL/min/1.73 m2). It seems that the most suitable cardiac parameter used in the prognostic classification of AL amyloidosis, independent of renal function, is TnI. It should be noted that a concentration of involved λ chains hada higher diagnostic power to assess the heart involvement, compared to the routinely used “cardiac parameters”, TnI and NT-proBNP. It can therefore be an additional parameter used to assess the presence of amyloid in the myocardium. A decrease in eGFR value influenced the change in the diagnostic cut-off points of the most analyzed laboratory parameters. Finally, it is concluded that lowering the eGFR value reduces the utility of laboratory parameters used in the prognostic classification of AL amyloidosis.
Safety and Efficacy of Laparoscopic Caudate Lobectomy: A Systematic Review
Authors: Dorovinis Panagiotis , Machairas Nikolaos , Kykalos Stylianos , Stamopoulos Paraskevas , Vernadakis Spyridon , Sotiropoulos Georgios C
Published: October 2021

Resection of the caudate lobe of the liver is considered a highly challenging type of liver resection due to the region’s intimacy with critical vascular structures and deep anatomic location inside the abdominal cavity. Laparoscopic resection of the caudate lobe is considered one of the most challenging laparoscopic liver procedures. The objective of our systematic review was to evaluate the safety, technical feasibility and main outcomes of laparoscopic caudate lobectomy LCL. A systematic review of the literature was undertaken for studies published until September 2021. A total of 20 studies comprising 221 patients were included. Of these subjects, 36% were women, whereas the vast majority of resections (66%) were performed for malignant tumors. Tumor size varied significantly between 2 and 160 mm in the largest diameter. The mean operative time was 210 min (range 60–740 min), and estimated blood loss was 173.6 mL (range 50–3600 mL). The median hospital length of stay LOS was 6.5 days (range 2–15 days). Seven cases of conversion to open were reported. The vast majority of patients (93.7%) underwent complete resection (R0) of their tumors. Thirty-six out of 221 patients developed postoperative complications, with 5.8% of all patients developing a major complication (Clavien–Dindo classification ≥ III).No perioperative deaths were reported by the included studies. LCL seems to be a safe and feasible alternative to open caudate lobectomy OCL in selected patients when undertaken in high-volume centers by experienced surgeons.
Effects of Robotic-Assisted Gait Training in Children and Adolescents with Cerebral Palsy: A Network Meta-Analysis
Authors: Olmos-Gómez Raquel , Gómez-Conesa Antonia , Calvo-Muñoz Inmaculada , López-López José A.
Published: October 2021

Gait disturbances are common in children and adolescents with cerebral palsy (CP). Robotic-assisted gait training (RAGT) is becoming increasingly widespread, and hence it is important to examine its effectiveness. A network meta-analysis (NMA) of clinical trials comparing treatments with RAGT vs. other physical therapy treatments was carried out. This study was conducted according to the NMA version of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA-NMA) guidelines and following the recommendations of the Cochrane Handbook for Systematic Reviews of Interventions. The outcome variables used were the D and E dimensions of the Gross Motor Function Measure (GMFM), gait speed, resistance, and stride length. Among 120 records, 8 trials were included. This NMA did not find statistically significant results for any of the comparisons examined in any of the outcomes studied and the magnitude of the effect size estimates was low or very low. Our NMA results should be interpreted with caution due to the high clinical heterogeneity of the studies included.
A Novel Multi-Dimensional Clinical Response Index Dedicated to Improving Global Assessment of Pain in Patients with Persistent Spinal Pain Syndrome after Spinal Surgery, Based on a Real-Life Prospective Multicentric Study (PREDIBACK) and Machine Learning Techniques
Authors: Rigoard Philippe , Ounajim Amine , Goudman Lisa , Louis Pierre-Yves , Slaoui Yousri , Roulaud Manuel , Naiditch Nicolas , Bouche Bénédicte , Page Philippe , Lorgeoux Bertille , Baron Sandrine , Charrier Elodie , Poupin Laure , Rannou Delphine , de Montgazon Géraldine Brumauld , Roy-Moreau Brigitte , Grimaud Nelly , Adjali Nihel , Nivole Kevin , Many Mathilde , David Romain , Wood Chantal , Rigoard Raphael , Moens Maarten , Billot Maxime
Published: October 2021

The multidimensionality of chronic pain forces us to look beyond isolated assessment such as pain intensity, which does not consider multiple key parameters, particularly in post-operative Persistent Spinal Pain Syndrome (PSPS-T2) patients. Our ambition was to produce a novel Multi-dimensional Clinical Response Index (MCRI), including not only pain intensity but also functional capacity, anxiety-depression, quality of life and quantitative pain mapping, the objective being to achieve instantaneous assessment using machine learning techniques. Two hundred PSPS-T2 patients were enrolled in the real-life observational prospective PREDIBACK study with 12-month follow-up and received various treatments. From a multitude of questionnaires/scores, specific items were combined, as exploratory factor analyses helped to create a single composite MCRI; using pairwise correlations between measurements, it appeared to more accurately represent all pain dimensions than any previous classical score. It represented the best compromise among all existing indexes, showing the highest sensitivity/specificity related to Patient Global Impression of Change (PGIC). Novel composite indexes could help to refine pain assessment by informing the physician’s perception of patient condition on the basis of objective and holistic metrics, and also by providing new insights regarding therapy efficacy/patient outcome assessments, before ultimately being adapted to other pathologies.
Mechanical and Microstructural Assessment of Inhomogeneities in Oxide Ceramic Matrix Composites Detected by Air-Coupled Ultrasound Inspection
Authors: Roßdeutscher Jan , Mechnich Peter , Flucht Ferdinand , Shi Yuan , Jemmali Raouf
Published: October 2021

Ceramic Matrix Composites (CMC) are promising materials for high-temperature applications where damage tolerant failure behavior is required. Non-destructive testing is essential for process development, monitoring, and quality assessment of CMC parts. Air-coupled ultrasound (ACU) is a fast and cost-efficient tool for non-destructive inspections of large components with respect to the detection of material inhomogeneities. Even though ACU inspection is usually used for visual inspection, the interpretation of C-scan images is often ambiguous with regard to critical defects and their impact on local material properties. This paper reports on a new approach to link the local acoustic damping of an oxide CMC plate obtained from ACU analysis with subsequent destructive mechanical testing and microstructural analyses. Local damping values of bending bars are extracted from ACU maps and compared with the results of subsequent resonant frequency damping analysis and 3-point bending tests. To support data interpretation, the homogeneous and inhomogeneous CMC areas detected in the ACU map are further analyzed by X-ray computed tomography and scanning electron microscopy. The results provide strong evidence that specific material properties such as Young’s modulus are not predictable from ACU damping maps. However, ACU shows a high, beneficial sensitivity for narrow but large area matrix cracks or delaminations, i.e., local damping is significantly correlated with specific properties such as shear moduli and bending strengths.
A Radioactive-Free Method for the Thorough Analysis of the Kinetics of Cell Cytotoxicity
Authors: Coronnello Claudia , Busà Rosalia , Cicero Luca , Comelli Albert , Badami Ester
Published: October 2021

The cytotoxic activity of T cells and Natural Killer cells is usually measured with the chromium release assay (CRA), which involves the use of 51Chromium (51Cr), a radioactive substance dangerous to the operator and expensive to handle and dismiss. The accuracy of the measurements depends on how well the target cells incorporate 51Cr during labelling which, in turn, depends on cellular division. Due to bystander metabolism, the target cells spontaneously release 51Cr, producing a high background noise. Alternative radioactive-free methods have been developed. Here, we compare a bioluminescence (BLI)-based and a carboxyfluorescein succinimidyl ester (CFSE)-based cytotoxicity assay to the standard radioactive CRA. In the first assay, the target cells stably express the enzyme luciferase, and vitality is measured by photon emission upon the addition of the substrate d-luciferin. In the second one, the target cells are labelled with CFSE, and the signal is detected by Flow Cytometry. We used these two protocols to measure cytotoxicity induced by treatment with NK cells. The cytotoxicity of NK cells was determined by adding increasing doses of human NK cells. The results obtained with the BLI method were consistent with those obtained with the CRA- or CFSE-based assays 4 hours after adding the NK cells. Most importantly, with the BLI assay, the kinetic of NK cells’ killing was thoroughly traced with multiple time point measurements, in contrast with the single time point measurement the other two methods allow, which unveiled additional information on NK cell killing pathways.
Energy Consumption Modeling for Underwater Gliders Considering Ocean Currents and Seawater Density Variation
Authors: Song Yang , Ye Huangjie , Wang Yanhui , Niu Wendong , Wan Xu , Ma Wei
Published: October 2021

Energy management is a critical and challenging factor required for efficient and safe operation of underwater gliders (UGs), and the energy consumption model (ECM) is indispensable. In this paper, a more complete ECM of UGs is established, which considers ocean currents, seawater density variation, deformation of the pressure hull, and asymmetry of gliding motion during descending and ascending. Sea trial data are used to make a comparison between ECMs with and without the consideration of ocean currents, and the results prove that the ECM that considers the currents has a significantly higher accuracy. Then, the relationship between energy consumption and multiple parameters, including gliding velocity relative to the current, absolute gliding angle, and diving depth, is revealed. Finally, a simple example is considered to illustrate the effects of the depth-averaged current on the energy consumption.
Development of a Nomogram Model for Treatment of Elderly Patients with Locoregionally Advanced Nasopharyngeal Carcinoma
Authors: Kou Jia , Zhang Lu-Lu , Yang Xing-Li , Wen Dan-Wan , Zhou Guan-Qun , Wu Chen-Fei , Xu Si-Si , Zheng Wei-Hong , Qi Zhen-Yu , Sun Ying , Lin Li
Published: October 2021

(1) Purpose: This study aims to explore risk-adapted treatment for elderly patients with locoregionally advanced nasopharyngeal carcinoma (LA-NPC) according to their pretreatment risk stratification and the degree of comorbidity. (2) Methods: A total of 583 elderly LA-NPC patients diagnosed from January 2011 to January 2018 are retrospectively studied. A nomogram for disease-free survival (DFS) is constructed based on multivariate Cox regression analysis. The performance of the model is evaluated by using the area under the curve (AUC) of the receiver operating characteristic curve and Harrell concordance index (C-index). Then, the entire cohort is divided into different risk groups according to the nomogram cutoff value determined by X-tile analysis. The degree of comorbidities is assessed by the Charlson Comorbidity Index (CCI). Finally, survival rates are estimated and compared by the Kaplan–Meier method and the log-rank test. (3) Results: A nomogram for DFS is constructed with T/N classification, Epstein-Barr virus DNA and albumin. The nomogram shows well prognostic performance and significantly outperformed the tumor-node-metastasis staging system for estimating DFS (AUC, 0.710 vs. 0.607; C-index, 0.668 vs. 0.585; both p < 0.001). The high-risk group generated by nomogram has significantly poorer survival compared with the low-risk group (3-year DFS, 76.7% vs. 44.6%, p < 0.001). For high-risk patients with fewer comorbidities (CCI = 2), chemotherapy combined with radiotherapy is associated with significantly better survival (p < 0.05) than radiotherapy alone. (4) Conclusion: A prognostic nomogram for DFS is constructed with generating two risk groups. Combining risk stratification and the degree of comorbidities can guide risk-adapted treatment for elderly LA-NPC patients.
Quantitative DNA Methylation Analysis and Epigenotype-Phenotype Correlations in Taiwanese Patients with Beckwith-Wiedemann Syndrome
Authors: Lin Hsiang-Yu , Lee Chung-Lin , Fran Sisca , Tu Ru-Yi , Chang Ya-Hui , Niu Dau-Ming , Chang Chia-Ying , Chiu Pao Chin , Chou Yen-Yin , Hsiao Hui-Pin , Yang Chia-Feng , Tsai Meng-Che , Chu Tzu-Hung , Chuang Chih-Kuang , Lin Shuan-Pei
Published: October 2021

Background: Beckwith-Wiedemann syndrome (BWS; OMIM 130650) is a rare overgrowth syndrome with tumor predisposition resulting from the abnormal expression or function of imprinted genes of the chromosome 11p15.5 imprinting gene cluster. The aim of this study was to identify the epigenotype-phenotype correlations of these patients using quantitative DNA methylation analysis. Methods: One hundred and four subjects with clinically suspected BWS were enrolled in this study. All of the subjects had been referred for diagnostic testing which was conducted using methylation profiling of H19-associated imprinting center (IC) 1 and KCNQ1OT1-associated IC2 in high-resolution melting analysis and methylation quantification with the MassARRAY assay. Correlations between the quantitative DNA methylation status and clinical manifestations of the enrolled subjects were analyzed. Results: Among the 104 subjects, 19 had IC2 hypomethylation, 2 had IC1 hypermethylation, and 10 had paternal uniparental disomy (pUPD). The subjects with IC2 hypomethylation were characterized by significantly more macroglossia but less hemihypertrophy compared to the subjects with pUPD (p < 0.05). For 19 subjects with IC2 hypomethylation, the IC2 methylation level was significantly different (p < 0.05) between the subjects with and without features including macroglossia (IC2 methylation level: 11.1% vs. 30.0%) and prenatal or postnatal overgrowth (8.5% vs. 16.9%). The IC2 methylation level was negatively correlated with birth weight z score (p < 0.01, n = 19) and birth height z score (p < 0.05, n = 13). For 36 subjects with clinically diagnosed BWS, the IC2 methylation level was negatively correlated with the BWS score (r = −0.592, p < 0.01). The IC1 methylation level showed the tendency of positive correlation with the BWS score without statistical significance (r = 0.137, p > 0.05). Conclusions: Lower IC2 methylation and higher IC1 methylation levels were associated with greater disease severity in the subjects with clinically diagnosed BWS. Quantitative DNA methylation analysis using the MassARRAY assay could improve the detection of epigenotype-phenotype correlations, which could further promote better genetic counseling and medical care for these patients.
Protein Aggregation of NPAS3, Implicated in Mental Illness, Is Not Limited to the V304I Mutation
Authors: Samardžija Bobana , Pavešić Radonja Aristea , Zaharija Beti , Bergman Mihaela , Renner Éva , Palkovits Miklós , Rubeša Gordana , Bradshaw Nicholas J.
Published: October 2021

An emerging phenomenon in our understanding of the pathophysiology of mental illness is the idea that specific proteins may form insoluble aggregates in the brains of patients, in partial analogy to similar proteinopathies in neurodegenerative diseases. Several proteins have now been detected as forming such aggregates in the brains of patients, including DISC1, dysbindin-1 and TRIOBP-1. Recently, neuronal PAS domain protein 3 (NPAS3), a known genetic risk factor for schizophrenia, was implicated through a V304I point mutation in a family with major mental illness. Investigation of the mutation revealed that it may lead to aggregation of NPAS3. Here we investigated NPAS3 aggregation in insular cortex samples from 40 individuals, by purifying the insoluble fraction of these samples and testing them by Western blotting. Strikingly, full-length NPAS3 was found in the insoluble fraction of 70% of these samples, implying that aggregation is far more widely spread than can be accounted for by this rare mutation. We investigated the possible mechanism of aggregation further in neuroblastoma cells, finding that oxidative stress plays a larger role than the V304I mutation. Finally, we tested to see if NPAS3 aggregation could also be seen in blood serum, as a more accessible tissue than the human brain for future diagnosis. While no indication of NPAS3 aggregation was seen in the serum, soluble NPAS3 was detected, and was more prevalent in patients with schizophrenia than in those with major depressive disorder or controls. Aggregation of NPAS3 therefore appears to be a widespread and multifactorial phenomenon. Further research is now needed to determine whether it is specifically enhanced in schizophrenia or other mental illnesses.
Inpatient Weight Restoration Treatment Is Associated with Decrease in Post-Meal Anxiety
Authors: Sjögren Magnus , Kizilkaya Ismail , Støving Rene Klinkby
Published: October 2021

Objective: Anorexia nervosa (AN) is characterized by weight loss, distorted body image with fear of becoming fat and associated with anxiety, especially in relation to food intake. Anxiety in relation to meals and weight restoration remains a major challenge in the treatment of AN. We examined the effects of inpatient weight restoration treatment on levels of post-meal anxiety using visual analogue scale (VAS) ratings in patients with AN. Materials: Thirty-two patients with AN, all part of the PROspective Longitudinal all-comer inclusion study on Eating Disorders (PROLED) were followed over eight weeks with baseline psychometric measures and weekly VAS anxiety self-scoring. Methods: Apart from the weekly body mass index (BMI) and VAS, patients were characterized at baseline using the Eating Disorder Examination Questionnaire (EDE-Q), Eating Disorder Inventory (EDI), Symptom Check List 92 (SCL-92), Major Depression Inventory (MDI), and Autism Quotient (AQ). Results: The results showed a significant time effect, Wilks Lambda = 0.523, F = 3.12, p < 0.05 (power of 0.862), indicating a reduction in VAS scores of anxiety from baseline to week 8. There was no effect of baseline medication or scores of MDI on the results. BMI increased from a mean of 15.16 (week 1) to 17.35 (week 8). In comparison, patients dropping out after only three weeks (n = 31) also had a trend toward a reduction in VAS anxiety (ns). Conclusions: Inpatient weight restoration treatment is associated with a decrease in post-meal anxiety in AN, an effect that occurs early and becomes clinically significant in patients who stay in treatment.
Spatial Distribution of Permafrost in the Xing’an Mountains of Northeast China from 2001 to 2018
Authors: Zhang Yanyu , Zang Shuying , Li Miao , Shen Xiangjin , Lin Yue
Published: October 2021

Permafrost is a key element of the cryosphere and sensitive to climate change. High-resolution permafrost map is important to environmental assessment, climate modeling, and engineering application. In this study, to estimate high-resolution Xing’an permafrost map (up to 1 km2), we employed the surface frost number (SFN) model and ground temperature at the top of permafrost (TTOP) model for the 2001–2018 period, driven by remote sensing data sets (land surface temperature and land cover). Based on the comparison of the modeling results, it was found that there was no significant difference between the two models. The performances of the SFN model and TTOP model were evaluated by using a published permafrost map. Based on statistical analysis, both the SFN model and TTOP model efficiently estimated the permafrost distribution in Northeast China. The extent of Xing’an permafrost distribution simulated by the SFN model and TTOP model were 6.88 × 105 km2 and 6.81 × 105 km2, respectively. Ground-surface characteristics were introduced into the permafrost models to improve the performance of models. The results provided a basic reference for permafrost distribution research at the regional scale.
Use of Quantitative Dried Blood Spots to Evaluate the Post-Vaccination Level of Neutralizing Antibodies against SARS-CoV-2
Authors: Marchand Alexandre , Roulland Ingrid , Semence Florian , Beck Olof , Ericsson Magnus
Published: October 2021

To combat the COVID-19 pandemic, vaccines against SARS-CoV-2 are now given to protect populations worldwide. The level of neutralizing antibodies following the vaccination will evolve with time and vary between individuals. Immunoassays quantifying immunoglobulins against the viral spike (S) protein in serum/plasma have been developed, but the need for venous blood samples could limit the frequency and scale of control in populations. The use of a quantitative dried blood spot (DBS) that can be self-collected would simplify this monitoring. The objective of this study was to determine whether a quantitative DBS device (Capitainer qDBS 10 µL) could be used in combination with an Elecsys anti-SARS-CoV-2 S immunoassay from Roche to follow the development and persistence of anti-S antibodies. This objective was carried out through two clinical studies. The first study investigated 14 volunteers who received two doses of the Comirnaty (Pfizer) vaccine. The levels of anti-S antibodies and the progression over time post-vaccination were studied for three months. The level of produced antibodies varied between subjects, but a similar trend was observed. The anti-S antibodies were highly stimulated by the second dose (×100) and peaked two weeks later. The antibody levels subsequently decreased and three months later were down to 65%. DBS proved to be sufficiently sensitive for use in evaluating the immune status against SARS-CoV-2 over a prolonged time. The second cohort was composed of 200 random patients from a clinical chemistry department in Stockholm. In this cohort, we had no information on previous COVID-19 infections or vaccination. Nevertheless, 87% of the subjects had anti-S immunoglobulins over 0.8 U/mL, and the bias between plasma and DBS proved to be variable, as was also seen in the first vaccination study.
Examining the Toxicity of α-Synuclein in Neurodegenerative Disorders
Authors: Shan Frank Y. , Fung Kar-Ming , Zieneldien Tarek , Kim Janice , Cao Chuanhai , Huang Jason H.
Published: October 2021

α-synuclein is considered the main pathological protein in a variety of neurodegenerative disorders, such as Parkinson’s disease, multiple system atrophy, and dementia with Lewy bodies. As of now, numerous studies have been aimed at examining the post-translational modifications of α-synuclein to determine their effects on α-synuclein aggregation, propagation, and oligomerization, as well as the potential cellular pathway dysfunctions caused by α-synuclein, to determine the role of the protein in disease progression. Furthermore, α-synuclein also appears to contribute to the fibrilization of tau and amyloid beta, which are crucial proteins in Alzheimer’s disease, advocating for α-synuclein’s preeminent role in neurodegeneration. Due to this, investigating the mechanisms of toxicity of α-synuclein in neurodegeneration may lead to a more proficient understanding of the timeline progression in neurodegenerative synucleinopathies and could thereby lead to the development of potent targeted therapies.
Activity and Function of the PRMT8 Protein Arginine Methyltransferase in Neurons
Authors: Dong Rui , Li Xuejun , Lai Kwok-On
Published: October 2021

Among the nine mammalian protein arginine methyltransferases (PRMTs), PRMT8 is unusual because it has restricted expression in the nervous system and is the only membrane-bound PRMT. Emerging studies have demonstrated that this enzyme plays multifaceted roles in diverse processes in neurons. Here we will summarize the unique structural features of PRMT8 and describe how it participates in various neuronal functions such as dendritic growth, synapse maturation, and synaptic plasticity. Recent evidence suggesting the potential role of PRMT8 function in neurological diseases will also be discussed.
Variable Stiffness Design and Multiobjective Crashworthiness Optimization for Collision Post of Subway Cab Cars
Authors: Guo Wei , Xu Ping , Yi Zhaofeng , Xing Jie , Zhao Hui , Yang Chengxing
Published: October 2021

This paper proposes a variable stiffness collision post (VSCP) structure based on a uniform stiffness collision post (USCP) structure and performs stiffness matching optimization for VSCPs. A collision post structure assembled in a subway front-end frame can maintain the living space and absorb a certain amount of the kinetic energy of an impact. The experiment was applied on USCP, and the finite element model was verified experimentally. To investigate the effects of the stiffness parameters of VSCP on the specific energy absorption response (SEA_VSCP) and the area of intrusion response (S_In), response surface models fitted from design of experiment were adopted with the finite element model. In addition, a multiobjective optimization design was realized by using the global response search method and a Pareto frontier sequence was generated, which was based on the developed response surface model. It was found that the optimal value of SEA_VSCP and S_In responses cannot be achieved at the same time. Finally, a grey relational analysis is propounded to attain a desirable balance between SEA_VSCP and S_In from the Pareto frontier sequence under constraints of the peak crash force of VSCP and energy absorption of the front-end of cab car. The optimization result shows that the crashworthiness of VSCP is better than that of USCP.
The Service Strategy and Influencing Factors of Online Recycling of Used Mobile Phones
Authors: Shen Liang , Fan Runjie , Yu Zhaoqing , Wang Yuyan
Published: October 2021

Currently, many online platforms participate in the recycling of waste products, and the online recycling of used mobile phones is especially popular. We explore the service strategy for and the factors influencing customers’ willingness of online recycling (CWOR) of used mobile phones. We develop a game model of a reverse supply chain comprising a mobile phone manufacturer (MPM) and an online recycling platform (ORP) to analytically examine the influencing factors of CWOR. We show that the MPM’s profit is mainly affected by CWOR and the ORP’s offered recycling service. The impacts of customers’ preferences of the recycling price and recycling service on the MPM’s profit depend on the service cost coefficient. The ORP’s profit is independent of its service cost and customers’ preference of the recycling service. However, the impact of the recycling commission is restricted by customers’ preference of the recycling price. We also use structural equation modelling to empirically show that recycling service and environmental consciousness positively affect CWOR, and environmental protection publicity enhances environmental consciousness. In addition, customers’ age and income moderate the recycling service–CWOR link. Our findings reveal that improving the recycling service can directly and indirectly promote the recycling of used mobile phones. These findings provide guidance to the government and businesses related to used mobile phone recycling.
On Andrews’ Partitions with Parts Separated by Parity
Authors: Alanazi Abdulaziz M. , Nyirenda Darlison
Published: October 2021

In this paper, we present a generalization of one of the theorems in Partitions with parts separated by parity introduced by George E. Andrews, and give its bijective proof. Further variations of related partition functions are studied resulting in a number of interesting identities.
Evaluation of Multivariate Adaptive Regression Splines and Artificial Neural Network for Prediction of Mean Sea Level Trend around Northern Australian Coastlines
Authors: Raj Nawin , Gharineiat Zahra
Published: October 2021

Mean sea level rise is a significant emerging risk from climate change. This research paper is based on the use of artificial intelligence models to assess and predict the trend on mean sea level around northern Australian coastlines. The study uses sea-level times series from four sites (Broom, Darwin, Cape Ferguson, Rosslyn Bay) to make the prediction. Multivariate adaptive regression splines (MARS) and artificial neural network (ANN) algorithms have been implemented to build the prediction model. Both models show high accuracy (R2 > 0.98) and low error values (RMSE < 27%) overall. The ANN model showed slightly better performance compared to MARS over the selected sites. The ANN performance was further assessed for modelling storm surges associated with cyclones. The model reproduced the surge profile with the maximum correlation coefficients ~0.99 and minimum RMS errors ~4 cm at selected validating sites. In addition, the ANN model predicted the maximum surge at Rosslyn Bay for cyclone Marcia to within 2 cm of the measured peak and the maximum surge at Broome for cyclone Narelle to within 7 cm of the measured peak. The results are comparable with a MARS model previously used in this region; however, the ANN shows better agreement with the measured peak and arrival time, although it suffers from slightly higher predictions than the observed sea level by tide gauge station.
Jet Impingement Cooling of a Rotating Hot Circular Cylinder with Hybrid Nanofluid under Multiple Magnetic Field Effects
Authors: Ayadi Badreddine , Selimefendigil Fatih , Alresheedi Faisal , Kolsi Lioua , Aich Walid , Said Lotfi Ben
Published: October 2021

The cooling performance of jet impinging hybrid nanofluid on a rotating hot circular cylinder was numerically assessed under the effects of multiple magnetic fields via finite element method. The numerical study was conducted for different values of Reynolds number (100≤Re≤300), rotational Reynolds number (0≤Rew≤800), lower and upper domain magnetic field strength (0≤Ha≤20), size of the rotating cylinder (2 w ≤r≤ 6 w) and distance between the jets (6 w ≤ H ≤ 16 w). In the presence of rotation at the highest speed, the Nu value was increased by about 5% when Re was increased from Re = 100 to Re = 300. This value was 48.5% for the configuration with the motionless cylinder. However, the rotations of the cylinder resulted in significant heat transfer enhancements in the absence or presence of magnetic field effects in the upper domain. At Ha1 = 0, the average Nu rose by about 175%, and the value was 249% at Ha1 = 20 when cases with the cylinder rotating at the highest speed were compared to the motionless cylinder case. When magnetic field strengths of the upper and lower domains are reduced, the average Nu decreases. The size of the cylinder is influential on the flow dynamics and heat transfer when the cylinder is rotating. An optimum value of the distance between the jets was obtained at H = 14 w, where the Nu value was highest for the rotating cylinder case. A modal analysis of the heat transfer dynamics was performed with the POD technique. As diverse applications of energy system technologies with impinging jets are available, considering the rotations of the cooled surface under the combined effects of using magnetic field and nanoparticle loading in heat transfer fluid is a novel contribution. The outcomes of the present work will be helpful in the initial design and optimization studies in applications from electronic cooling to convective drying, solar power and many other systems.
Laxative Effects of a Standardized Extract of Dendropanax morbiferus H. Léveille Leaves on Experimental Constipation in Rats
Authors: Na Ju-Ryun , Lee Ki Hoon , Kim Eun , Hwang Kwontack , Na Chang-Su , Kim Sunoh
Published: October 2021

Background and Objectives: This study aimed at investigating the laxative effects of a standardized aqueous extract of Dendropanax morbiferus H. Lév. on two different constipation rat models. Materials and Methods: Animal studies were conducted with low-fiber diet-induced and loperamide-induced constipation animal models, and isolated colons were used in ex vivo analysis to determine the changes in colonic motility caused by D. morbiferus H. Lév. leaf extract (DPL). Results: The results showed that DPL administration significantly improved certain reduced fecal parameters (number, weight, and water content of the stools) in a both low-fiber diet and loperamide-induced constipation models without adverse effects of diarrhea. The laxative effect of DPL was confirmed to improve the charcoal excretion time upon DPL treatment in a low-fiber diet or loperamide-induced constipation model through gastrointestinal (GI) motility evaluation using the charcoal meal test. In addition, when DPL was administered to RAW264.7 cells and loperamide-induced constipation model rats, the production of prostaglandin E2 (PGE2) increased significantly in cells and tissue. Furthermore, DPL dose-dependently stimulated the spontaneous contractile amplitude and frequency of the isolated rat colon. Conclusion: Although our study did not provide information on the acute or chronic toxicity of DPL, our results demonstrated that DPL can effectively promote defecation frequency and rat colon contraction, providing scientific evidence to support the use of DPL as a therapeutic application. However, further toxicity studies of DPL are needed prior to the initiation of clinical trials and clinical applications.
Prognostic Value of the PROFUND Index for 30-Day Mortality in Acute Heart Failure
Authors: Méndez-Bailón Manuel , Iguarán-Bermúdez Rosario , López-García Lidia , Sánchez-Sauce Beatriz , Pérez-Mateos Pablo , Barrado-Cuchillo Julia , Villar-Martínez Miguel , Fernández-Castelao Santiago , García-Klepzig Jose , Fuentes-Ferrer Manuel , García-García Alejandra , Vilacosta Isidre , de Miguel-Yanes José , Casas-Rojo José , Calvo-Manuel Elpidio , Andres Emmanuel , Lorenzo-Villalba Noel ,   on behalf of the Heart Failure and Pluripathology Groups from the Spanish National Society of Internal Medicine
Published: October 2021

Background and Objectives: The prevalence and incidence of heart failure (HF) have been increasing in recent years as the population ages. These patients show a distinct profile of comorbidity, which makes their care more complex. In recent years, the PROFUND index, a specific tool for estimating the mortality rate at one year in pluripathology patients, has been developed. The aim of this study was to evaluate the prognostic value of the PROFUND index and of in-hospital and 30-day mortality after discharge of patients admitted for acute heart failure (AHF). Materials and Methods: A prospective multicenter longitudinal study was performed that included patients admitted with AHF and ≥2 comorbid conditions. Clinical, analytical, and prognostic variables were collected. The PROFUND index was collected in all patients and rates of in-hospital and 30-day mortality after discharge were analyzed. A bivariate analysis was performed with quantitative variables between patients who died and those who survived at the 30-day follow-up. A logistic regression analysis was performed with the variables that obtained statistical significance in the bivariate analysis between deceased and surviving subjects. Results: A total of 128 patients were included. Mean age was 80.5 +/− 9.98 years, and women represented 51.6%. The mean PROFUND index was 5.26 +/− 4.5. The mortality rate was 8.6% in-hospital and 20.3% at 30 days. Preserved left ventricular ejection fraction was found in 60.9%. In the sample studied, there were patients with a PROFUND score < 7 predominated (89 patients (70%) versus 39 patients (31%) with a PROFUND score ≥ 7). Thirteen patients (15%) with a PROFUND score < 7 died versus the 13 (33%) with a PROFUND score ≥ 7, p = 0.03. Twelve patients (15%) with a PROFUND score < 7 required readmission versus 12 patients (35%) with a PROFUND score ≥ 7, p = 0.02. The ROC curve of the PROFUND index for in-hospital mortality and 30-day follow-up in patients with AHF showed AUC 0.63, CI: 95% (0.508–0.764), p <0.033. Conclusions: The PROFUND index is a clinical tool that may be useful for predicting short-term mortality in elderly patients with AHF. Further studies with larger simple sizes are required to validate these results.