bluuebunny/crossref_metadata_2025 huggingface dataset:
An Improved Phase Deviation Discriminator for Carrier Synchronization of APSK Signal in Satellite-to-Ground Communication Systems
Authors: Shen Yuyao , Shi Xuesen , Zhao Shiqi , Wang Yongqing
Published: May 2022

The conventional phase deviation discriminator used in the decision feedback loop for carrier synchronization of APSK signal requires symbol decision for every constellation symbol. When the number of constellation symbols used for coherent integration becomes larger, the complexity would increase greatly. To solve this problem, this work proposes an improved phase deviation discriminator based on symbol decisions. It firstly executes doubling frequency on the APSK signal to reduce the modulation order and eliminate the modulation phase of the received signal. Then, it rotates the constellation clockwise and selects the constellation symbols that on the X-axis to execute coherent integration and phase deviation extraction. Compared to the conventional discriminator in the decision feedback loop, the proposed discriminator can reduce the symbol decision times and steps. Therefore, when a large number of constellation symbols are used for coherent integration, it can reduce the computational complexity significantly. Moreover, with a large number of constellation symbols, the proposed discriminator achieves better BER and RMSE performance than other existing feedback loops.
Machine Learning Models and Videos of Facial Regions for Estimating Heart Rate: A Review on Patents, Datasets, and Literature
Authors: Pagano Tiago Palma , Santos Victor Rocha , Bonfim Yasmin da Silva , Paranhos José Vinícius Dantas , Ortega Lucas Lemos , Sá Paulo Henrique Miranda , Nascimento Lian Filipe Santana , Winkler Ingrid , Nascimento Erick Giovani Sperandio
Published: May 2022

Estimating heart rate is important for monitoring users in various situations. Estimates based on facial videos are increasingly being researched because they allow the monitoring of cardiac information in a non-invasive way and because the devices are simpler, as they require only cameras that capture the user’s face. From these videos of the user’s face, machine learning can estimate heart rate. This study investigates the benefits and challenges of using machine learning models to estimate heart rate from facial videos through patents, datasets, and article review. We have searched the Derwent Innovation, IEEE Xplore, Scopus, and Web of Science knowledge bases and identified seven patent filings, eleven datasets, and twenty articles on heart rate, photoplethysmography, or electrocardiogram data. In terms of patents, we note the advantages of inventions related to heart rate estimation, as described by the authors. In terms of datasets, we have discovered that most of them are for academic purposes and with different signs and annotations that allow coverage for subjects other than heartbeat estimation. In terms of articles, we have discovered techniques, such as extracting regions of interest for heart rate reading and using video magnification for small motion extraction, and models, such as EVM-CNN and VGG-16, that extract the observed individual’s heart rate, the best regions of interest for signal extraction, and ways to process them.
Decay on Cyclic CO2 Capture Performance of Calcium-Based Sorbents Derived from Wasted Precursors in Multicycles
Authors: Gong Dehong , Zhang Zhongxiao , Zhao Ting
Published: May 2022

In order to obtain the cheap waste calcium-based sorbent, three wasted CaCO3 precursors, namely carbide slag, chicken eggshells, and analytical reagent-grade calcium carbonate, were selected and prepared at 700 °C to form calcium-based sorbents for CO2 capture. TGA was used to test the CO2 uptake performance of each calcium-based sorbent in 20 cycles. To identify the decay mechanism of CO2 uptake with an increasing number of cycles, all calcium-based sorbents were characterized by using XRF, XRD, and N2 adsorption. The specific surface area of calcium-based sorbents was used to redefine the formula of cyclic carbonation reactivity decay. The carbonation conversion rate of three calcium-based sorbents exhibited a decreasing trend as the cycle number increased. Chicken eggshells exhibited the most significant decrease rate (over 50% compared with Cycle 1), while carbide slag and analytical reagent-grade calcium carbonate showed a flat linear decline trend. The specific surface area of the samples was used to calculate carbonation conversion for an infinite number of cycles. The carbonation conversion rates of three calcium-based sorbents were estimated to decrease to 0.2898, 0.1455, and 0.3438 mol/mol, respectively, after 100 cycles.
Low and Ultra-Low Temperature District Heating Equipped by Heat Pumps—An Analysis of the Best Operative Conditions for a Swiss Case Study
Authors: Toffanin Riccardo , Caputo Paola , Belliardi Marco , Curti Vinicio
Published: May 2022

The manuscript analyses the management of low and ultra-low-temperature district heating systems (DHS) coupled with centralised and decentralised heat pumps. Operative conditions are defined in order to satisfy the heating needs without overloading the electric grid. The results are achieved by dynamic simulations, based on a real DHS located in southern Switzerland. At the building level, the heating needs are estimated considering real data and simultaneous energy simulations. Two DHS configurations, alternatives to the existing one, are simulated and suitable parameters for the management of the DHS are selected. The global performance of the two DHS is evaluated by KPIs also including the flexibility and the impact on the electric peak due to heat pumps. The achieved results are discussed providing suggestions for the stakeholders involved in DHS management for an optimal matching of the electric grid and thermal networks towards a reduction of the peak power. The rule-based control strategies defined allow the expected electric peak shaving and load levelling, conversely, the yearly energy consumptions are lightly increased and have to be further investigated. The outcomes demonstrate a global better performance of the ultra-low temperature DHS in terms of response to the applied control strategies and of energy savings.
Microwave Pre-Treatment and Blending of Biomass Pellets for Sustainable Use of Local Energy Resources in Energy Production
Authors: Goldšteins Linards , Dzenis Māris Gunārs , Šints Viesturs , Valdmanis Raimonds , Zaķe Maija , Arshanitsa Alexandr
Published: May 2022

In the present study analysis of co-firing microwave (MW) pre-treated biomass pellets of different origins (wood and wheat straw), with raw pellets (wood, straw, and peat), to control and improve thermochemical conversion of biomass blends and achieve a sustainable use of local energy resources in energy production has been carried out. Effects of MW pre-treatment regimes and composition of blends were studied experimentally using measurements of the weight loss of blends, the yield of volatiles, flame temperature, total heat output from the device, and composition of products. It was found that co-firing MW pre-treated and raw biomass pellets promotes synergistic interaction between components of blends by increasing mass loss rate, the intensity of which depends on the proximate composition of pellets, MW pre-treatment regime and mass fraction of pre-treated pellets in the blend. The most effective synergistic interaction was found when co-firing pre-treated straw or wood pellets with raw peat, which increased the yield of combustible volatiles and heat output from the device as well as improved the composition of emissions. The least effective synergistic interaction was observed when co-firing pre-treated straw with raw wood pellets. Main factors that influenced the thermal and chemical conversion of MW pre-treated blends are discussed considering the effects of MW pre-treatment on the structural changes, elemental and chemical composition, and heating value of pre-treated pellets.
Possibilities of Adapting a Free-Cooling System in an Existing Commercial Building
Authors: Rabczak Sławomir , Nowak Krzysztof
Published: May 2022

Increasingly, the exploitation of buildings involves the need for comprehensive management of all systems operating within it, their continuous monitoring, both for the safety of users and to mainly optimize the operating parameters of systems that are largely dependent on each other, interact with each other. This is mainly associated with the need for the most energy-efficient management of energy consumption, which has the largest share of operating costs. The main cost for the facilities is the cooling aspect, which is significant, and for this reason, it is necessary to analyze the possibility of its minimization. One of the possible systems to consider in order to reduce the consumption of cooling energy in the facility is the application of free-cooling technology, which is based on the use of the natural cooling capacity of the outside air for cooling rooms with high heat gains. The application of the free cooling system for facilities with a relatively stable cooling load, with significant heat gains from equipment is justified due to the possibility of maintaining low room temperatures, below the comfort value for people, for a long period of time. The problem arises when a large proportion of the heat gains are from people. In such a situation, cooling of rooms is possible but requires a large amount of ventilation air, which is already a serious limitation on the one hand because of the existing air conditioning equipment, lack of possibility to increase the ventilation air stream; on the other hand, the low heat capacity of the air itself, compared to, e.g., water systems used for cooling of the facility or multisplit systems. These limitations put a question mark over the possibility of adapting the technology of free cooling for the existing facilities, where the majority of heat gains come from people. It is not possible in such objects to lower the room temperature below the limit values for the sake of thermal comfort, which in itself limits the possibilities of using air as a natural cooling agent in such objects. Based on the measurement data obtained from the object, it was decided to check the possibilities of applying free-cooling technology in a large shopping facility and obtaining an answer as to what extent it is possible to reduce the power of cooling units with the application of free-cooling exchangers.
Changes in the Concentrations of Trace Elements and Supply of Nutrients to Silver Fir (Abies alba Mill.) Needles as a Bioindicator of Industrial Pressure over the Past 30 Years in Świętokrzyski National Park (Southern Poland)
Authors: Świercz Anna , Świątek Bartłomiej , Pietrzykowski Marcin
Published: May 2022

The aim of this study was to identify changes in the concentrations of elements in fir assimilation organs over the past 30 years in order to assess fir reactions as a bioindicator of changes in the functioning of Central European fir forest ecosystems under conditions of reduced anthropogenic emissions. In particular, we selected the example of the Świętokrzyskie Mountains (Świętokrzyski National Park located in the northern range of Abies alba Mill.). The research was carried out in the “Łysica-Święty Krzyż” area under strict protection, including multi-species and uneven-aged tree stands with a complex structure, dominance of beech and fir, and numerous admixtures of other tree species. A decrease in the concentrations of pollutants in fir needles indicates a significant reduction in pressure on the environment and an improvement in the conditions of ecosystems in 2018 compared with those in 1986. In the period of more than 30 years between the sets of research, the concentrations of lead in fir needles decreased threefold and those of sulfur decreased twofold. A significant increase in the concentrations of cadmium, copper, manganese, and zinc in the three-year-old needles showed that they are a good indicator of environmental pollution with trace elements and may be used in biomonitoring.
Enhancing Short-Term Sales Prediction with Microblogs: A Case Study of the Movie Box Office
Authors: Zhao Jie , Xiong Fangwei , Jin Peiquan
Published: May 2022

Microblogs are one of the major social networks in people’s daily life. The increasing amount of timely microblog data brings new opportunities for enterprises to predict short-term product sales based on microblogs because the daily microblogs posted by various users can express people’s sentiments on specific products, such as movies and books. Additionally, the social influence of microblogging platforms enables the rapid spread of product information, implemented by users’ forwarding and commenting behavior. To verify the usefulness of microblogs in enhancing the prediction of short-term product sales, in this paper, we first present a new framework that adopts the sentiment and influence features of microblogs. Then, we describe the detailed feature computation methods for sentiment polarity detection and influence measurement. We also implement the Linear Regression (LR) model and the Support Vector Regression (SVR) model, selected as the representatives of linear and nonlinear regression models, to predict short-term product sales. Finally, we take movie box office predictions as an example and conduct experiments to evaluate the performance of the proposed features and models. The results show that the proposed sentiment feature and influence feature of microblogs play a positive role in improving the prediction precision. In addition, both the LR model and the SVR model can lower the MAPE metric of the prediction effectively.
Whey Beverage Emulsified System as Carrying Matrix of Fennel Seed Extract Obtained by Supercritical CO2 Extraction: Impact of Thermosonication Processing and Addition of Prebiotic Fibers
Authors: Urango Adela Cristina Martinez , Neves Maria Isabel Landim , Meireles Maria Angela A. , Silva Eric Keven
Published: May 2022

Whey beverages that were enriched with fructooligosaccharides (FOS) and xylooligosaccharides (XOS) were used for carrying Foeniculum vulgare extract that was obtained by the supercritical CO2 extraction technique to produce novel functional products. Fennel-based whey beverages were subjected to thermosonication processing (100, 200, and 300 W at 60 °C for 15 min) to verify the performance of the dairy colloidal system for protecting the bioactive fennel compounds. The impacts of thermosonication processing on the quality attributes of the functional whey beverages were examined according to their droplet size distribution, microstructure, kinetic stability, color parameters, browning index, total phenolic content (TPC), and antioxidant capacity by DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2-Azino-bis-(3-ethylbenzothiazoline)-6-sulphonic acid) assays. The enrichment of the whey beverages with FOS and XOS did not affect their kinetic stability. However, the addition of prebiotic dietary fibers contributed to reducing the mean droplet size due to the formation of whey protein–FOS/XOS conjugates. The thermosonication treatments did not promote color changes that were discernible to the human eye. On the other hand, the thermosonication processing reduced the kinetic stability of the beverages. Overall, the colloidal dairy systems preserved the antioxidant capacity of the fennel seed extract, regardless of thermosonication treatment intensity. The whey beverages enriched with FOS and XOS proved to be effective carrying matrices for protecting the lipophilic bioactive fennel compounds.
Anti-Oxidative Effect of Pu-erh Tea in Animals Trails: A Systematic Review and Meta-Analysis
Authors: Yang Chiung-Ying , Hung Kuang-Chen , Yen Yea-Yin , Liao Hung-En , Lan Shou-Jen , Lin Hsin-Cheng
Published: May 2022

This study adopted systematic literature review and meta-analysis methodology to explored anti-oxidative effect of pu-erh tea. Study authors have systemically searched seven databases up until 21 February 2020. In performing the literature search on the above-mentioned databases, the authors used keywords of pu-erh AND (superoxide dismutase OR glutathione peroxidase OR malondialdehyde). Results derived from meta-analyses showed statistically significant effects of pu-erh tea on reducing serum MDA levels (SMD, −4.19; 95% CI, −5.22 to −3.15; p < 0.001; I2 = 93.67%); increasing serum SOD levels (SMD, 2.41; 95% CI, 1.61 to 3.20; p < 0.001; I2 = 91.36%); and increasing serum GSH-Px levels (SMD, 4.23; 95% CI, 3.10 to 5.36; p < 0.001; I2 = 93.69%). Results from systematic review and meta-analyses validated that various ingredients found in pu-erh tea extracts had anti-oxidation effects, a long-held conventional wisdom with limited supporting evidence.
Study on Volatile Profiles, Polycyclic Aromatic Hydrocarbons, and Acrylamide Formed in Welsh Onion (Allium fistulosum L.) Fried in Vegetable Oils at Different Temperatures
Authors: Kim Hye-Min , Park Min-Kyung , Mun Soo-Jeong , Jung Mun-Yhung , Lee Sang-Mi , Kim Young-Suk
Published: May 2022

Welsh onion (Allium fistulosum L.) is widely used in diverse Asian cuisines, especially in stir-fried and deep-fried foods. This study investigated the effects of different temperatures (140, 165, and 190 °C) and types of the vegetable frying oil (soybean, corn, canola, and palm oils) on the formation of volatile profiles and hazardous compounds [polycyclic aromatic hydrocarbons (PAHs) and acrylamide] in Welsh onion. Specific volatile chemical groups such as aldehydes, sulfur-containing compounds, and furans/furanones were major volatiles in Welsh onion fried (WOF). The composition of aldehydes and sulfur-containing compounds decreased, while those of furans/furanones increased when WOF samples were exposed to higher temperatures. At 190 °C, PAHs were detected at lower than the EU maximum tolerable limit (the sum of 4 PAHs, <10 µg/kg), and acrylamide was detected below 36.46 μg/kg. The integrated study of both the quality and safety properties can provide fundamental data for the industrial processing of WOF.
Effects of UV-B and Water Deficit on Aroma Precursors in Grapes and Flavor Release during Wine Micro-Vinification and Consumption
Authors: Zhu Yifan , Sun Meng , Harrison Roland , Jordan Brian , Creasy Glen , Hofmann Rainer
Published: May 2022

UV-B radiation and water availability can affect amino acids(AAs) concentration in berries, resulting in the evolution of aroma compounds during alcoholic fermentation. This study investigated the effects of UV-B exposure and water availability onwine aroma compounds in Pinot noir, focusing on the role of AAs in the process.Enhanced UV-B radiation significantly decreased total AA concentrations and most individual AAs inberries and wines, while water deficitincreased some individual AAsin wines. Higher alcohols, fatty acids, esters, monoterpenes, and C13-norisoprenoids were affected by UV-B interaction with water deficit in wines. These results suggested individual or combined UV-B exposure and water deficit had direct effects on fruit AAs, leading to significant differences in some wine aroma compounds.
Trace Elements as Contaminants and Nutrients
Authors: Couto Cristina , Pinto Edgar , Almeida Agostinho
Published: May 2022

Knowledge about trace elements has evolved remarkably in recent decades, both in terms of their metabolism and their functions [...]
Effect of High-Hydrostatic-Pressure Processing and Storage Temperature on Sliced Iberian Dry-Cured Sausage (“Salchichón”) from Pigs Reared in Montanera System
Authors: Ramírez Rosario , Trejo Antonia , Delgado-Adámez Jonathan , Martín-Mateos María Jesús , García-Parra Jesús
Published: May 2022

The top-quality “salchichón” (a fermented dry-cured sausage) is manufactured from Iberian pigs reared outdoors. This work aims to evaluate the effect of hydrostatic high pressure (HHP) and storage temperature on sliced vacuum-packaged top-quality Iberian “salchichón”. Two types of “salchichón” (S1 and S2, manufactured at different companies) were processed at 600 MPa for 8 min and stored at 4 and 20 °C for 180 days. Microbiological, physicochemical, and sensory changes were evaluated. Microbiological counts were reduced by HHP treatment and also generally decreased during storage at both temperatures. Lightness and redness of slices decreased during storage at 20 °C, while yellowness values increased. Changes in color were also observed in sensory analysis of the dry-cured sausages. HHP increased lipid and protein oxidation values in S1, whereas protein oxidation increased at 20 °C in S2. S1 was more affected by HHP while S2 was more affected by the temperature of storage. Therefore, despite both products belonging to the same commercial category, slight differences in the composition of both products and/or differences in packaging determined a different behavior after HHP treatment and during storage at different temperatures.
A Study on the Time–Effect and Dose–Effect Relationships of Polysaccharide from Opuntia dillenii against Cadmium-Induced Liver Injury in Mice
Authors: Liu Ting , Li Bianli , Zhou Xin , Chen Huaguo
Published: May 2022

The purpose of this study was to evaluate the protective effect of Opuntia dillenii (Ker-Gaw) Haw. polysaccharide (ODP) against cadmium-induced liver injury. Cadmium chloride (CdCl2) was used to construct a mice evaluation model, and the indicators chosen included general signs, liver index, biochemical indicators, blood indicators, and pathological changes. A dose of 200 mg/kg ODP was applied to the mice exposed to cadmium for different lengths of time (7, 14, 21, 28, and 35 days). The results showed that CdCl2 intervention led to slow weight growth (reduced by 13–20%); liver enlargement; significantly increased aspartate aminotransferase (AST, 45.6–52.0%), alanine aminotransferase (ALT, 26.6–31.3%), and alkaline phosphatase (ALP, 38.2–43.1%) levels; and significantly decreased hemoglobin (HGB, 13.1–15.2%), mean corpuscular hemoglobin (MCH, 16.5–19.3%), and mean corpuscular hemoglobin concentrations (MCHC, 8.0–12.7%) (p < 0.01). In addition, it led to pathological features such as liver cell swelling, nuclear exposure, central venous congestion, apoptosis, and inflammatory cell infiltration. The onset of ODP anti-cadmium-induced liver injury occurred within 7 days after administration, and the efficacy reached the highest level after continuous administration for 14 days, a trend that could continue until 35 days. Different doses (50, 100, 200, 400, and 600 mg/kg) of ODP have a certain degree of protective effect on cadmium-induced liver injury, showing a good dose–effect relationship. After 28 days of administration of a 200 mg/kg dose, all pathological indicators were close to normal values. These findings indicated that ODP had positive activity against cadmium-induced liver injury and excellent potential for use as a health food or therapeutic drug.
Prominent Follicular Keratosis in Multiple Intestinal Atresia with Combined Immune Deficiency Caused by a TTC7A Homozygous Mutation
Authors: Diociaiuti Andrea , Caruso Roberta , Ricci Silvia , De Vito Rita , Strocchio Luisa , Castiglia Daniele , Zambruno Giovanna , El Hachem May
Published: May 2022

Multiple intestinal atresia with combined immune deficiency (MIA-CID) is an autosomal recessive syndrome due to mutations in the TTC7A gene implicated in the polarization of intestinal and thymic epithelial cells. MIA-CID is lethal in the first year of life in the majority of patients. Dermatological manifestations have been reported in a few cases. We describe a child affected with MIA-CID due to a previously unreported TTC7A homozygous missense mutation. Surgery for bowel occlusion was performed in the first days of life. The patient was totally dependent on parenteral nutrition since birth and presented severe diarrhea and recurrent infections. He underwent hematopoietic stem cell transplantation at 17 months with complete donor engraftment and partial immunity improvement. In the second year of life, he progressively developed diffuse papular follicular keratoses on ichthyosiform skin, nail clubbing, and subungual hyperkeratosis. Histopathology showed hyperkeratosis with follicular plugging and scattered apoptotic keratinocytes, visualized at an ultrastructural examination. Our findings expand the spectrum of dermatological manifestations which can develop in MIA-CID patients. Examination of further patients will allow defining whether keratinocyte apoptosis is also a disease feature.
Genome-Wide Characterization and Expression Analysis of GATA Transcription Factors in Response to Methyl Jasmonate in Salvia miltiorrhiza
Authors: Li Haiyan , Liu Tianrang , Wang Biao , Li Hongbo
Published: May 2022

Salvia miltiorrhiza is an important medicinal plant, which is mainly used for treatment of cardiovascular and cerebrovascular diseases. GATA transcription factors are evolutionarily conser-ved proteins that play essential roles in biological process of plants. In this study, we systematically characterized the GATA transcription factors in S. miltiorrhiza. A total 28 SmGATA genes were identified and divided into four subfamilies based on phylogenetic analysis and domain. SmGATA genes being clustered into a subfamily have similar conserved motifs and exon-intron patterns, and unevenly distribute on eight chromosomes of S. miltiorrhiza. Tissue-specific expression analysis based on transcriptome datasets showed that the majority of SmGATA genes were preferentially expressed in roots. Under methyl jasmonate (MeJA) treatment, the quantitative real-time PCR (qRT-PCR) analysis indicated that several SmGATA genes in roots showed distinct upregulation post-MeJA treatment, especially SmGATA08, which was highly responsive to MeJA, and might be involved in the jasmonate signal, thereby affecting root growth, development, tolerance to various stresses, or secondary metabolites biosynthesis. The study found that several SmGATAs, like SmGATA08, are highly responsive to MeJA, indicating that these SmGATAs might be vital in the biosynthesis of tanshinones and phenolic acids by regulating the response to MeJA in S. miltiorrhiza. Our results laid the foundation for understanding their biological roles and quality improvement in S. miltiorrhiza.
Discovery of Stishovite in the Prismatine-Bearing Granulite from Waldheim, Germany: A Possible Role of Supercritical Fluids of Ultrahigh-Pressure Origin
Authors: Thomas Rainer , Davidson Paul , Rericha Adolf , Recknagel Ulrich
Published: May 2022

For the first time in the sixty years since the synthesis of stishovite, we report unambiguous evidence of stishovite formed in the deep Earth. A minimum pressure of about 7.5 GPa at 1000 °C is necessary for the formation of stishovite, corresponding to a depth of about 230 km. In this manuscript we report the identification of stishovite along with coesite as inclusions in mineral grains from the Waldheim granulite. This implies that the stishovite was transported upwards, probably very rapidly to a depth of about 130 km, corresponding to the highest pressure indicated by newly identified coesite in the prismatine of the Waldheim granulite, and continuing up to the depth of emplacement of the Waldheim prismatine granulite. The analysis of the Raman spectra obtained from a metastable trapped stishovite micro-crystal show that all the diagnostic Raman bands are present. However, given the metastability of the stishovite at room temperatures and pressures, this mineral breaks down step-by-step into stable polymorphs, first coesite and then quartz and cristobalite, during the Raman stimulation. The rare coesite crystals in prismatine have also resulted from the irreversible transformation from stishovite. Although the Waldheim occurrence may be unique, we suggest that Raman analysis of co-trapped crystals in similar deep-seated rocks, an area of limited previous research, may prove an important innovation in the study of mantle processes.
Understanding Attitudes, Social Norms, and Behaviors of a Cohort of Post-Operative Nurses Related to Pain and Pain Management
Authors: Ayaz Nur Pinar , Sherman Deborah Witt
Published: May 2022

Despite advances in surgical techniques and peri-operative care, pain is a significant symptom post-operatively. The purpose of this study was to examine the attitudes, social norms, and behaviors of a cohort of nurses of various ethnic and cultural backgrounds regarding pain and pain assessment and management. The design was a descriptive qualitative study guided by Theory of Planned Behavior and the Theory of Transcultural Nursing. The sample included six registered nurses (RNs) (two Hispanic, two African American, and two Caucasian), along with patients of the same and different cultural and ethnic backgrounds in the post-operative unit within 48 h of surgery. The nurses’ results indicated that nurses assess pain severity and patient treatment preferences yet do not conduct a comprehensive pain assessment and have limited knowledge of non-pharmacologic and complementary pain therapies. Despite knowledge of the patient’s pain history, tolerance, and cultural background, the nurses believed that “all patients should be treated the same” and were concerned about the use of opioids beyond the first 24–48 h post-operatively due to the risk of addiction and professional ramifications related to opioid administration. The conclusions are that ongoing education is needed regarding comprehensive pain assessment and pharmacologic, non-pharmacologic, and complementary pain therapies during the post-operative period. Discussion is needed regarding the importance of diversity and equity as it relates to cultural competence within the context of pain assessment and management to provide patient-centered individualized care.
Genetic Characterization of the Factor VIII Gene in a Cohort of Colombian Patients with Severe Hemophilia A with Inhibitors
Authors: Doncel Samuel Sarmiento , Mosquera Gina Alejandra Diaz , Pelaez Ronald Guillermo , Cortes Javier Mauricio , Rico Carol Agudelo , Cadavid Francisco Javier Meza , Plazas Nelson Ramirez , Amar Ivan Alfredo Perdomo , Siado Jorge Enrique Peña , Rey Fabian Andres Parrado , Montaño Cesar Alberto , Villadiego Alexys Maza
Published: May 2022

Hemophilia A is an X-linked bleeding disorder caused by mutations in the FVIII gene. Genetic factors have been shown to be a risk factor for the development of inhibitors. We aimed to identify the specific variations of the FVIII gene of patients with hemophilia A with inhibitors and their association with the inhibitor titer. Methods: Cross-sectional descriptive study. We included 12 Colombian patients from a health care provider, “Integral Solutions SD”, who underwent analysis of genetic material (DNA), which was reported by the Molecular Hemostasis Laboratory in Bonn, Germany. Results: All of these patients were diagnosed with severe hemophilia A with inhibitors; ages ranged between 6 and 48 years, with a median age of 13.5 years. Molecular analysis showed the inversion of intron 22 in six patients (50.0%), a small duplication in two patients (16.7%), the inversion of intron 1 in one patient (8.3%), a large deletion (8.3%), a nonsense mutation (8.3%) and a splice-site (8.3%), findings similar to those of other studies. A total of 58.3% of the patients presented inversion mutations with a high risk of developing inhibitors A total of 83.3% of the evaluated patients presented null mutations; however the presence of high inhibitor titers was 66.7%. The most frequent mutation was the inversion intron 22. Knowing the type of mutation and its association as a risk factor for generating inhibitors invites us to delve into other outcomes such as residual values of coagulation FVIII as well as its impact on the half-life of the exogenous factor applied in prophylaxis.
A Tomato Pomace Enriched Gluten-Free Ready-to-Cook Snack’s Nutritional Profile, Quality, and Shelf Life Evaluation
Authors: Rehal Jagbir , Aggarwal Poonam , Dhaliwal Inderpreet , Sharma Meenakshi , Kaushik Prashant
Published: May 2022

Attempts were undertaken to design a quick ready-to-cook gluten-free snack utilizing finger millet and potato flour (50:50) as well as tomato pomace due to the restricted availability of gluten-free snack goods in the Indian market. The nutritional content of the food and its general acceptability, cooking characteristics, and storage stability were all tested. The addition of tomato pomace had a distinct influence on the product’s color and hardness. Additionally, it resulted in a significant reduction in the amount of oil used, cooking loss, and frying time required. With a high acceptance level, the snack supplemented with 10% tomato pomace was determined to be the most optimal formulation. When the same substance was subjected to FTIR analysis, it was discovered that it retained all the important functional groups required for sustaining antioxidant activity. It also displayed high storage stability, a desirable overall acceptance score, and a very promising nutritional profile, all of which would benefit the product’s end users.
The Effects of Plant Health Status on the Community Structure and Metabolic Pathways of Rhizosphere Microbial Communities Associated with Solanum lycopersicum
Authors: Adedayo Afeez , Fadiji Ayomide , Babalola Olubukola
Published: May 2022

Powdery mildew disease caused by Oidium neolycopersici is one of the major diseases affecting tomato production in South Africa. Interestingly, limited studies exist on how this disease affects the community structure microbial communities associated with tomato plants employing shotgun metagenomics. In this study, we assess how the health status of a tomato plant affects the diversity of the rhizosphere microbial community. We collected soil samples from the rhizosphere of healthy (HR) and diseased (DR; powdery mildew infected) tomatoes, alongside bulk soil (BR), extracted DNA, and did sequencing using shotgun metagenomics. Our results demonstrated that the rhizosphere microbiome alongside some specific functions were abundant in HR followed by DR and bulk soil (BR) in the order HR > DR > BR. We found eighteen (18) bacterial phyla abundant in HR, including Actinobacteria, Acidobacteria, Aquificae, Bacteroidetes, etc. The dominant fungal phyla include; Ascomycota and Basidiomycota, while the prominent archaeal phyla are Thaumarchaeota, Crenarchaeota, and Euryarchaeota. Three (3) bacteria phyla dominated the DR samples; Bacteroidetes, Gemmatimonadetes, and Thermotoga. Our result also employed the SEED subsystem and revealed that the metabolic pathways involved were abundant in HR. The α-diversity demonstrates that there is no significant difference among the rhizosphere microbiomes across the sites, while β-diversity demonstrated a significant difference.
The Effect of Climate Change on the Water Supply and Hydraulic Conditions in the Upper Pejibaye River Basin, Cartago, Costa Rica
Authors: Watson-Hernández Fernando , Guzmán-Arias Isabel , Chavarría-Pizarro Laura , Quesada-Alvarado Francisco
Published: May 2022

The consequences of climate change have challenged researchers to generate models and projections to understand climate behavior under different scenarios. In Costa Rica, as in other countries, climate-change (CC) models and projections are essential to make decisions about the management of natural resources, mainly water. To understand climate change’s impact on hydraulic parameters such as velocity, depth, and river surface area, we studied the Pejibaye river basin, located in Jiménez in Cartago, Costa Rica. This watershed is characterized by having more than 90% of its surface area covered by forest. We used the precipitation and temperature data from meteorological stations (2000 to 2009) and climate-change scenarios (2000–2099) to predict the response of the basin in different periods. First, we calibrated (NSE = 0.77) and validated (NSE = 0.81) the HBV hydrological model using ten years of daily data from 2000 to 2009. The climate-change data (2000–2099) were incorporated into the calibrated HBV model. This allowed us to determine the impact of CC on the basin water regime for the periods 2040–2059 (CCS1) and 2080–2099 (CCS2). The IBER mathematical model was used to determine the changes in the hydraulic variables of the river flow. For the CCS1, we determined a 10.9% decrease in mean velocity and a 0.1-meter decrease in depth, while for CCS2, the effect will be an 11.3% reduction in mean velocity and a 0.14-meter decrease in depth. The largest decreases in river surface area per kilometer will occur in May (1710 m2) for CCS1 and April (2250 m2) for CCS2.
Revising Cadastral Data on Land Boundaries Using Deep Learning in Image-Based Mapping
Authors: Fetai Bujar , Grigillo Dejan , Lisec Anka
Published: May 2022

One of the main concerns of land administration in developed countries is to keep the cadastral system up to date. The goal of this research was to develop an approach to detect visible land boundaries and revise existing cadastral data using deep learning. The convolutional neural network (CNN), based on a modified architecture, was trained using the Berkeley segmentation data set 500 (BSDS500) available online. This dataset is known for edge and boundary detection. The model was tested in two rural areas in Slovenia. The results were evaluated using recall, precision, and the F1 score—as a more appropriate method for unbalanced classes. In terms of detection quality, balanced recall and precision resulted in F1 scores of 0.60 and 0.54 for Ponova vas and Odranci, respectively. With lower recall (completeness), the model was able to predict the boundaries with a precision (correctness) of 0.71 and 0.61. When the cadastral data were revised, the low values were interpreted to mean that the lower the recall, the greater the need to update the existing cadastral data. In the case of Ponova vas, the recall value was less than 0.1, which means that the boundaries did not overlap. In Odranci, 21% of the predicted and cadastral boundaries overlapped. Since the direction of the lines was not a problem, the low recall value (0.21) was mainly due to overly fragmented plots. Overall, the automatic methods are faster (once the model is trained) but less accurate than the manual methods. For a rapid revision of existing cadastral boundaries, an automatic approach is certainly desirable for many national mapping and cadastral agencies, especially in developed countries.
Functions and Mechanisms of Pro-Lysyl Oxidase Processing in Cancers and Eye Pathologies with a Focus on Diabetic Retinopathy
Authors: Trackman Philip C. , Peymanfar Yaser , Roy Sayon
Published: May 2022

Lysyl oxidases are multifunctional proteins derived from five lysyl oxidase paralogues (LOX) and lysyl oxidase-like 1 through lysyl oxidase-like 4 (LOXL1–LOXL4). All participate in the biosynthesis of and maturation of connective tissues by catalyzing the oxidative deamination of lysine residues in collagens and elastin, which ultimately results in the development of cross-links required to function. In addition, the five LOX genes have been linked to fibrosis and cancer when overexpressed, while tumor suppression by the propeptide derived from pro-LOX has been documented. Similarly, in diabetic retinopathy, LOX overexpression, activity, and elevated LOX propeptide have been documented. The proteolytic processing of pro-forms of the respective proteins is beginning to draw attention as the resultant peptides appear to exhibit their own biological activities. In this review we focus on the LOX paralogue, and what is known regarding its extracellular biosynthetic processing and the still incomplete knowledge regarding the activities and mechanisms of the released lysyl oxidase propeptide (LOX-PP). In addition, a summary of the roles of both LOX and LOX-PP in diabetic retinopathy, and brief mentions of the roles for LOX and closely related LOXL1 in glaucoma, and keratoconus, respectively, are included.
Profile of Innate Immunity in Gilthead Seabream Larvae Reflects Mortality upon Betanodavirus Reassortant Infection and Replication
Authors: García-Álvarez Miguel Ángel , Arizcun Marta , Chaves-Pozo Elena , Cuesta Alberto
Published: May 2022

Historically, gilthead seabream (Sparus aurata) has been considered a fish species resistant to nervous necrosis virus (NNV) disease. Nevertheless, mortality in seabream hatcheries, associated with typical clinical signs of the viral encephalopathy and retinopathy (VER) disease has been confirmed to be caused by RGNNV/SJNNV reassortants. Because of this, seabream larvae at 37 and 86 days post-hatching (dph) were infected by immersion with RGNNV/SJNNV and SJNNV/RGNNV reassortants under laboratory conditions, and mortality, viral replication and immunity were evaluated. Our results show that gilthead seabream larvae, mainly those at 37 dph, are susceptible to infection with both NNV reassortant genotypes, with the highest impact from the RGNNV/SJNNV reassortant. In addition, viral replication occurs at both ages (37 and 86 dph) but the recovery of infective particles was only confirmed in 37 dph larvae,; this value was also highest with the RGNNV/SJNNV reassortant. Larvae immunity, including the expression of antiviral, inflammatory and cell-mediated cytotoxicity genes, was affected by NNV infection. Levels of the natural killer lysin (Nkl) peptide were increased in SJNNV/RGNNV-infected larvae of 37 dph, though hepcidin was not. Our results demonstrate that the seabream larvae are susceptible to both NNV reassortants, though mainly to RGNNV/SJNNV, in an age-dependent manner.
Acidic and Alkaline Conditions Affect the Growth of Tree Peony Plants via Altering Photosynthetic Characteristics, Limiting Nutrient Assimilation, and Impairing ROS Balance
Authors: Aung Theint Thinzar , Shi Fengrui , Zhai Yanning , Xue Jingqi , Wang Shunli , Ren Xiuxia , Zhang Xiuxin
Published: May 2022

Exposure to acidic and alkaline conditions were found to cause the excess accumulation of reactive oxygen species in tree peony, thereby causing damage and inhibiting plant growth and development. The activities of antioxidant enzymes were also found to be significantly up-regulated, especially under alkaline conditions; this explained why tree peony is better adapted to alkaline than to acidic conditions. Through pairwise comparisons, 144 differentially expressed genes (DEGs) associated with plant growth, photosynthesis, and stress were identified. The DEGs related to stress were up-regulated, whereas the remaining DEGs were almost all down-regulated after acid and alkaline treatments. The nutrient assimilation was greatly inhibited. Chlorophyll synthesis genes were suppressed, and chlorophyll content was reduced. The development and structures of stomata and chloroplasts and the transcription of related genes were also influenced. Among photosynthesis-related DEGs, electron transport chains were the most sensitive. The suppressed expression of photosynthesis genes and the reduced light-harvesting capacity, together with the impairment of chloroplasts and stomata, finally led to a sharp decrease in the net photosynthetic rate. Carbohydrate accumulation and plant biomass were also reduced. The present study provides a theoretical basis for the response mechanisms of tree peony to adverse pH conditions and enriches knowledge of plant adaptation to alkaline conditions.
Decreased BAFF Receptor Expression and Unaltered B Cell Receptor Signaling in Circulating B Cells from Primary Sjögren’s Syndrome Patients at Diagnosis
Authors: Neys Stefan F. H. , Verstappen Gwenny M. , Bootsma Hendrika , Kroese Frans G. M. , Hendriks Rudi W. , Corneth Odilia B. J.
Published: May 2022

Animal models of autoimmunity and human genetic association studies indicate that the dysregulation of B-cell receptor (BCR) signaling is an important driver of autoimmunity. We previously showed that in circulating B cells from primary Sjögren’s syndrome (pSS) patients with high systemic disease activity, protein expression of the BCR signaling molecule Bruton’s tyrosine kinase (BTK) was increased and correlated with T-cell infiltration in the target organ. We hypothesized that these alterations could be driven by increased B-cell activating factor (BAFF) levels in pSS. Here, we investigated whether altered BCR signaling was already present at diagnosis and distinguished pSS from non-SS sicca patients. Using (phospho-)flow cytometry, we quantified the phosphorylation of BCR signaling molecules, and investigated BTK and BAFF receptor (BAFFR) expression in circulating B cell subsets in an inception cohort of non-SS sicca and pSS patients, as well as healthy controls (HCs). We found that both BTK protein levels and BCR signaling activity were comparable among groups. Interestingly, BAFFR expression was significantly downregulated in pSS, but not in non-SS sicca patients, compared with HCs, and correlated with pSS-associated alterations in B cell subsets. These data indicate reduced BAFFR expression as a possible sign of early B cell involvement and a diagnostic marker for pSS.
Identifying the Molecular Mechanisms and Types of Cell Death Induced by bio- and pyr-Silica Nanoparticles in Endothelial Cells
Authors: Solarska-Ściuk Katarzyna , Adach Kinga , Fijałkowski Mateusz , Haczkiewicz-Leśniak Katarzyna , Kulus Michał , Olbromski Mateusz , Glatzel-Plucińska Natalia , Szelest Oskar , Bonarska-Kujawa Dorota
Published: May 2022

The term “nanosilica” refers to materials containing ultrafine particles. They have gained a rapid increase in popularity in a variety of applications and in numerous aspects of human life. Due to their unique physicochemical properties, SiO2 nanoparticles have attracted significant attention in the field of biomedicine. This study aimed to elucidate the mechanism underlying the cellular response to stress which is induced by the exposure of cells to both biogenic and pyrogenic silica nanoparticles and which may lead to their death. Both TEM and fluorescence microscopy investigations confirmed molecular changes in cells after treatment with silica nanoparticles. The cytotoxic activity of the compounds and intracellular RNS were determined in relation to HMEC-1 cells using the fluorimetric method. Apoptosis was quantified by microscopic assessment and by flow cytometry. Furthermore, the impact of nanosilica on cell migration and cell cycle arrest were determined. The obtained results compared the biological effects of mesoporous silica nanoparticles extracted from Urtica dioica L. and pyrogenic material and indicated that both types of NPs have an impact on RNS production causing apoptosis, necrosis, and autophagy. Although mesoporous silica nanoparticles did not cause cell cycle arrest, at the concentration of 50 μg/mL and higher they could disturb redox balance and stimulate cell migration.
Effects of Sodium-Glucose Co-Transporter-2 Inhibitors on Pancreatic β-Cell Mass and Function
Authors: Nakamura Akinobu
Published: May 2022

Sodium-glucose co-transporter-2 inhibitors (SGLT2is) not only have antihyperglycemic effects and are associated with a low risk of hypoglycemia but also have protective effects in organs, including the heart and kidneys. The pathophysiology of diabetes involves chronic hyperglycemia, which causes excessive demands on pancreatic β-cells, ultimately leading to decreases in β-cell mass and function. Because SGLT2is ameliorate hyperglycemia without acting directly on β-cells, they are thought to prevent β-cell failure by reducing glucose overload in this cell type. Several studies have shown that treatment with an SGLT2i increases β-cell proliferation and/or reduces β-cell apoptosis, resulting in the preservation of β-cell mass in animal models of diabetes. In addition, many clinical trials have shown that that SGLT2is improve β-cell function in individuals with type 2 diabetes. In this review, the preclinical and clinical data regarding the effects of SGLT2is on pancreatic β-cell mass and function are summarized and the protective effect of SGLT2is in β-cells is discussed.