bluuebunny/crossref_metadata_2025 huggingface dataset:
Is There Really a Sex Recession? Period and Cohort Effects on Sexual Inactivity Among American Men, 2006–2019
Authors: Bozick Robert
Published: November 2021

There has been a growing concern among researchers and media commentators that men in the United States may be increasingly less sexually active, creating a form of a “sex recession.” Using 14 years of survey data from men in the National Survey of Family Growth (2006–2019), this study assesses whether such concerns are warranted. Cross-classified mixed-effects models are estimated to ascertain whether there is evidence of a population-wide sex recession among men due to secular conditions specific to different time periods, or if birth cohorts that comprise the male population at any given point in time are exhibiting distinct patterns of sexual behavior. The analysis finds no evidence of a population-wide sex recession among men. Rates of sexual inactivity among men have been constant across the time series, but those born between 2000 and 2004 had significantly higher rates of sexual inactivity than previous birth cohorts did at the same age. Additionally, men who are unemployed and/or living at home with their parents are more likely to refrain from sexual intercourse than their peers who are employed and/or living independently of their parents.
The Mondragon Case: Companies Addressing Social Impact and Dialogic Methodologies
Authors: Morlà-Folch Teresa , Aubert Simon Adriana , de Freitas Ana Burgués , Hernández-Lara Ana Beatriz
Published: November 2021

Mondragon Cooperative (MC) is one of the most outstanding examples of worker cooperatives in history. It has maintained its cooperative values along its trajectory, being competitive in the international market since 1956, under the motto: ‘Humanity at work’. Recently, scientific research has focused on impact, which has led to the emergence of the Successful Cooperative Actions (SCAs). Based on the case of MC, the scientific literature has identified key actions of this business model that can be transferred to other corporate contexts. Using a methodological approach, it is key to identify the SCAs that MC implements, which could apply to other cooperatives with similar principles but not the same success. This study aims to analyse the methodological innovation in qualitative research when the aim is focused on the transferability of the social impact of MC to other contexts. This paper shows that research focussing on the identification of SCAs modifies qualitative methodology by focussing on the transferability of actions to obtain social impact.
Abdominal aortic endograft infection with Clostridium difficile
Authors: Lyle Cara A , Caputo Francis J
Published: November 2021

Objectives

The increase in endovascular aortic aneurysm repair has led to increasing incidence of aortic endograft infections. Additionally, more atypical organisms are being identified as pathogens. We report on a rare aortic endograft infection to further characterize and understand these infections.

Methods

We report a Clostridium difficile culture–positive aortic endograft infection in an 82-year-old male 3 years after endovascular abdominal aortic aneurysm repair.

Results

The patient underwent successful open, complete explant of his endograft and in-situ repair using a rifampin-soaked Dacron graft. He continues to do well.

Conclusions

Aortic endograft infections are a complex problem further complicated by rare and virulent infections. Unless the patient is at prohibitive risk, the management of infected aortic endografts is surgical graft explant and in-situ or extra-anatomic reconstruction due to the exceedingly high mortality rate with non-operative management.
Towards understanding of policy transfer and policy learning in adult education in the context of United Kingdom
Authors: Kersh Natasha , Laczik Andrea
Published: December 2021

Over the past decade, adult education and Vocational Education and Training (VET) in the UK context have been strongly affected by the implications of unstable political, social and economic situations, specifically in relation to social and economic inclusion of vulnerable young adults. This paper argues, that the development of policy transfer and policy learning in adult education has been characterised by its multidimensional nature and has been influenced by the implications of contemporary global challenges, country-specific priorities and the European agenda on inclusion. The notion of policy learning in adult education highlights the complex interdependencies between policies and practices. We will endeavour to consider how the complex interplays between country-specific priorities, global discourses and the European agenda on active citizenship (AC) contribute to national policies and practices for social inclusion of young adults in the UK context. Selected case studies will demonstrate the ways this policy agenda transfers into specific programmes for vulnerable young adults.
Decolonising recognition of prior learning – The drawbacks of policy mimicking
Authors: Werquin Patrick
Published: November 2021

Recognition of prior learning features at the top of the policy agenda in many countries because recognition of prior learning (RPL) has proven effective in some parts of the world; and there is much policy learning taking place. Therefore, many countries want to gain the advantages associated with RPL. It brings benefits to individual end-users and countries as a whole. Whether RPL should be identically implemented everywhere is more questionable. This paper proposes a reflexion based on experience: It looks at what RPL is about, at what it took to implement the validation des acquis de l’expérience (VAE) system in France and its potential transferability. While it is important to learn from one another, transporting a system from one national context to another is unlikely to work because the necessary social and cultural context is never transferred along with the technical apparatus. This is not new, but this paper emphasises the need for appropriate adaption whenever systems are borrowed.
Developing epistemic impartiality to deliver on justice in higher education South Africa
Authors: Kumalo Siseko H
Published: December 2021

In South Africa, the scholarship of epistemic justice has taken on an historical gaze with higher education framed as a social institution that might ameliorate the historical traumas of colonialism. Undoing the legacies of colonialism has been framed as the democratisation of the knowledge project. Using the White Paper 3 of 1997 that posits academic freedom, institutional autonomy and public accountability as fundamental to institutional governance, in part I of this analysis I broadened public accountability to include the social, political and economic factors that inhibit or act as catalyst to the attainment of educational desire. In this second part publication, I am interested in developing and proposing epistemic impartiality. This concept is developed from Mitova’s proposition of ‘decolonising knowledge without too much relativism’, which ultimately fosters epistemic justice through rigorously scrutinising each epistemic tradition. My suggestion is that epistemic impartiality enables dialogue between divergent traditions.
How to approach childhood obesity
Authors: Patterson Rosemarie , Sornalingam Sangeetha , Hannah Romanie , Cooper Max
Published: December 2021

Childhood obesity has a rising prevalence and is associated with significant morbidity, social and psychological consequences. In England, 21% of children aged between 10 and 11 are obese, with the highest rates being among children from lower socioeconomic groups. Primary care is responsible for managing most children who are overweight or obese. Consultations should generate positive conversations about childhood obesity and rule out underlying conditions and co-morbidities. Families may need support to recognise contributing factors and should be encouraged to identify simple, achievable strategies to promote healthier choices. Follow-up is essential, and families should be referred on to appropriate services for ongoing support.
7-Tesla MRI Evaluation of the Knee, 25 Years after Cartilage Repair Surgery: The Influence of Intralesional Osteophytes on Biochemical Quality of Cartilage
Authors: Janssen M.P.F. , Peters M.J.M. , Steijvers-Peeters E.G.M. , Szomolanyi P. , Jutten E.M.C. , van Rhijn L.W. , Peterson L. , Lindahl A. , Trattnig S. , Emans P.J.
Published: November 2021

Objective

To evaluate the morphological and biochemical quality of cartilage transplants and surrounding articular cartilage of patients 25 years after perichondrium transplantation (PT) and autologous chondrocyte transplantation (ACT) as measured by ultra-high-field 7-Tesla (7T) magnetic resonance imaging (MRI) and to present these findings next to clinical outcome.

Design

Seven PT patients and 5 ACT patients who underwent surgery on the femoral condyle between 1986 and 1996 were included. Patient-reported outcome measures (PROMs) were assessed by the clinical questionnaires: Knee injury and Osteoarthritis Outcome Score (KOOS), International Knee Documentation Committee (IKDC), and Visual Analogue Scale (VAS) for knee pain. The morphological (MOCART score) and biochemical quality (glycosaminoglycans [GAGs] content and collagen integrity) of cartilage transplants and surrounding articular cartilage were analyzed by 7T MRI. The results of the PT and ACT patients were compared. Finally, a detailed morphological analysis of the grafts alone was performed.

Results

No statistically significant difference was found for the PROMs and MOCART scores of PT and ACT patients. Evaluation of the graft alone showed poor repair tissue quality and high prevalence of intralesional osteophyte formation in both the PT and ACT patients. Penetration of the graft surface by the intralesional osteophyte was related to biochemically damaged opposing tibial cartilage; GAG content was significantly lower in patients with an osteophyte penetrating the graft surface.

Conclusions

Both PT and ACT patients have a high incidence of intralesional osteophyte formation 25 years after surgery. The resulting biochemical damage to the opposing tibial cartilage might be dependent on osteophyte morphology.
Id reaction and allergic contact dermatitis post-picosecond laser tattoo removal: A case report
Authors: Wong Ian TY , Cheung Larry WK
Published: November 2021

The novel picosecond lasers have emerged as a mainstay device in laser tattoo removal alongside Q-switch lasers, considered the gold standard in the field. Here, we present a 45-year-old female who developed a severe reaction to both her treated and untreated tattoos after two picosecond laser treatments and subsequent widespread eczematous eruption. Skin biopsies revealed findings consistent with hypersensitivity to exogenous red pigment. The clinicopathologic findings were consistent with an id reaction (autoeczematization) associated with allergic contact dermatitis to tattoo pigment. This case report highlights the potential for tattoo hypersensitivity following picosecond laser treatment and the dilemma associated with tattoo removal in sensitized patients. Additional therapeutic approaches are needed to provide patients with a safe means of tattoo removal while mitigating the risk of hypersensitivity reactions.
Re-evaluating the diagnostic efficacy of PSA as a referral test to detect clinically significant prostate cancer in contemporary MRI-based image-guided biopsy pathways
Authors: Lophatananon Artitaya , Light Alexander , Burns-Cox Nicholas , Maccormick Angus , John Joseph , Otti Vanessa , McGrath John , Archer Pete , Anning Jonathan , McCracken Stuart , Page Toby , Muir Ken , Gnanapragasam Vincent J
Published: December 2021

Introduction:

Modern image-guided biopsy pathways at diagnostic centres have greatly refined the investigations of men referred with suspected prostate cancer. However, the referral criteria from primary care are still based on historical prostate-specific antigen (PSA) cut-offs and age-referenced thresholds. Here, we tested whether better contemporary pathways and biopsy methods had improved the predictive utility value of PSA referral thresholds.

Methods:

PSA referral thresholds, age-referenced ranges and PSA density (PSAd) were assessed for positive predictive value (PPV) in detection of clinically significant prostate cancer (csPCa – histological ⩾ Grade Group 2). Data were analysed from men referred to three diagnostics centres who used multi-parametric magnetic resonance imaging (mpMRI)-guided prostate biopsies for disease characterisation. Findings were validated in a separate multicentre cohort.

Results:

Data from 2767 men were included in this study. The median age, PSA and PSAd were 66.4 years, 7.3 ng/mL and 0.1 ng/mL2, respectively. Biopsy detected csPCa was found in 38.7%. The overall area under the curve (AUC) for PSA was 0.68 which is similar to historical performance. A PSA threshold of ⩾ 3 ng/mL had a PPV of 40.3%, but this was age dependent (PPV: 24.8%, 32.7% and 56.8% in men 50–59 years, 60–69 years and ⩾ 70 years, respectively). Different PSA cut-offs and age-reference ranges failed to demonstrate better performance. PSAd demonstrated improved AUC (0.78 vs 0.68, p < 0.0001) and improved PPV compared to PSA. A PSAd of ⩾ 0.10 had a PPV of 48.2% and similar negative predictive value (NPV) to PSA ⩾ 3 ng/mL and out-performed PSA age-reference ranges. This improved performance was recapitulated in a separate multi-centre cohort ( n = 541).

Conclusion:

The introduction of MRI-based image-guided biopsy pathways does not appear to have altered PSA diagnostic test characteristics to positively detect csPCa. We find no added value to PSA age-referenced ranges, while PSAd offers better PPV and the potential for a single clinically useful threshold (⩾0.10) for all age groups.

Level of evidence:

IV
Cystadenoma of the seminal vesicle – Potential diagnostic pitfall: A case report and literature review
Authors: Franca Raduan Ahmed , Crocetto Felice , Pandolfo Savio Domenico , Ponsiglione Andrea , Franzese Corrado Antonio , Imbriaco Massimo , Imbimbo Ciro , Insabato Luigi
Published: December 2021

Background:

Cystadenomas of the seminal vesicles are benign cystic tumours, currently classified into the group of mixed epithelial-stromal tumours (MEST). Primary tumours arising in the seminal vesicles are quite rare, benign tumours being rarer than malignant ones, with only a few cases reported in the English literature since 1944. Cystadenomas represent a potential diagnostic pitfall, as they can be clinically confused with malignant neoplasms and histologically with non-neoplastic inflammatory lesions or other cystic tumours, even malignant.

Objective and methods:

We report a case of a 23-year-old man presenting with a cystic mass of the seminal vesicle, clinically suspected to be a malignant neoplasm. On pathological examination, a diagnosis of cystadenoma was made. Diagnostic criteria to make a diagnosis of this tumour are questionable. Therefore, we carried out a literature review in attempt to compare clinico-pathological findings of 28 cases reported in the English literature as cystadenomas of the seminal vesicle.

Results:

Only 28 cases of cystadenomas of seminal vesicles were reported in the English literature. The available data show the difficulty to rule out malignancy preoperatively in a patient with a solid-cystic mass in the pelvic region, and that morphological findings observed were often reported incompletely.

Conclusions:

The current classification appears debatable, as well as histopathological criteria to diagnose a cystadenoma of the seminal vesicle. For the proper management of these lesions, a multidisciplinary approach is mandatory.

Level of evidence:

Not applicable
Comparison of efficacy of tranexamic acid irrigation versus intravenous injection for preventing blood loss in percutaneous nephrolithotomy
Authors: Choudhury Sunirmal , Dutta Avisek , Pal Dilip Kumar
Published: December 2021

Purpose:

Bleeding is the most dreadful and distressing complication of percutaneous nephrolithotomy, which can cause significant morbidity. Intravenous use of tranexamic acid to reduce bleeding complication is widely established. Our aim is to compare the result between the two groups using tranexamic acid intravenous and in irrigation solution.

Materials and methods:

A total of 160 patients were enrolled in this study. Patients were randomly allocated to two groups. Group 1 received 0.1% tranexamic acid solution (1000 mg in 1 L of irrigation solution (normal saline)). Group 2 received 1 g tranexamic acid intravenously in intraoperative period.

Results:

The mean estimated fall in haematocrit, mean estimated total blood loss and mean operative time in tranexamic acid irrigation group is significantly less compared to intravenous tranexamic acid group. No significant difference was found with regards to stone clearance.

Conclusion:

We found that tranexamic acid irrigation was safe and associated with reduced blood loss as compared to intravenous tranexamic acid.
Development, Validation, and Internal Consistency of the Positive Psychology-Based Acceptance of Illness Scale (PAIS): A Culturally-Appropriate Japanese Measure
Authors: Suzuki Ai , Ishikawa Hirono , Okada Masafumi
Published: November 2021

Acceptance of illness has been shown to improve the well-being of individuals suffering from chronic illnesses. We developed a new acceptance of illness scale, “Positive Psychology-Based Acceptance of Illness Scale: PAIS,” for HTLV-1 associated myelopathy (HAM) patients, one of the intractable neurological diseases with a chronic course. The validity and internal consistency of this PAIS were examined in 260 HAM patients in Japan. Exploratory factor analysis extracted four factors with 21 items: “Loving myself as I am”; “Experiencing the grace of illness”; “Feeling a loving connection with others”; and “Developing as a mature person”. Cronbach’s α was .83 to .88, except for Factor 4 which was slightly low at 0.64, and internal consistency was confirmed. A significant correlation was found between the PAIS and the index of convergent validity. The study’s findings are an important step toward a more comprehensive concept of acceptance of illness.
Haken conference interpreters in Japan: Exploring status through the sociology of work and of professions
Authors: Giustini Deborah
Published: November 2021

This article investigates the professional status of conference interpreters in Japan, by focusing on interpreters employed as haken, that is, dispatched temporary workers. Combining the perspectives of interpreting studies and the sociology of work, it addresses both internal and external factors upholding interpreters’ status: expertise, autonomy, and authority, on one hand, and social and market dynamics, on the other hand. It provides a thick empirical analysis of status-related factors by drawing on fieldwork data in Japan, including 46 interviews with interpreters and 7 interviews with agency managers. The findings show that internal and external factors intertwine in limiting or upholding interpreters’ status recognition. Despite their expertise and qualifications, conference interpreters in Japan have limited control over their work, because of clients’ expectations of subordination. Furthermore, the monopoly of agencies in the Japanese market constrains their professional visibility. Last but not least, interpreters’ employment as temporary workers and the disproportionate feminisation of the category contribute to societal perceptions of interpreting as an insecure and unrewarding occupation. The findings bear practical implications for the advocacy of interpreters’ status and its betterment in Japan.
Selection of suitable bentonite and the influence of various acids on the preparation of a special clay for the removal of trace olefins from aromatics
Authors: Rouhani Hadi , Farhadi Fatola , Akbari Kenari Mahsa , Eskandari Effat , Ramakrishna Seeram
Published: December 2021

Acid-activated clays are inexpensive materials that are used extensively in the removal of unsaturated compounds on an industrial scale. The performance of bentonitic clays in removing these compounds relies heavily on the types of raw clays and acids used in the activation process. In this work, we report on the removal of olefins from aromatic streams by bentonitic clays activatedviatwo different routes. After preliminary tests of four different natural clays, the best clay was selected in terms of it having high swelling index, cation-exchange capacity, specific surface area and suspension stability values. Activation was achieved with hydrochloric acid (HCl) and sulfuric acid (H2SO4), and olefin removal was evaluated after holistic clay characterization by means of X-ray diffraction, X-ray fluorescence, Brunauer–Emmett–Teller (BET) specific surface area analysis, ζ-potential analysis, Fourier-transform infrared (FTIR) spectroscopy after treatment with pyridine, scanning electron microscopy and transmission electron microscopy. The increased basal spacing, replacement of H+with interlayer cations and retained structural stability of the clay after acid treatment contributed to the improvement of olefin removal for HCl-activated clay. The HCl-activated clay was more efficient in terms of olefin removal than its H2SO4-activated counterpart, removing up to 90% of olefin components after 40 h. Based on pyridine-FTIR spectra and quantitative measurement of the acidic properties of the samples, HCl treatment increased the total number acid sites (Brønsted and Lewis) by approximately ninefold compared to the pristine natural clay and by approximately fourfold compared to the H2SO4-activated clay.
C5a and C5aR1 are key drivers of microvascular platelet aggregation in clinical entities spanning from aHUS to COVID-19
Authors: Aiello Sistiana , Gastoldi Sara , Galbusera Miriam , Ruggenenti Piero , Portalupi Valentina , Rota Stefano , Rubis Nadia , Liguori Lucia , Conti Sara , Tironi Matteo , Gamba Sara , Santarsiero Donata , Benigni Ariela , Remuzzi Giuseppe , Noris Marina
Published: December 2021

Unrestrained activation of the complement system till the terminal products, C5a and C5b-9, plays a pathogenetic role in acute and chronic inflammatory diseases. In endothelial cells, complement hyperactivation may translate into cell dysfunction, favoring thrombus formation. The aim of this study was to investigate the role of the C5a/C5aR1 axis as opposed to C5b-9 in inducing endothelial dysfunction and loss of antithrombogenic properties. In vitro and ex vivo assays with serum from patients with atypical hemolytic uremic syndrome (aHUS), a prototype rare disease of complement-mediated microvascular thrombosis due to genetically determined alternative pathway dysregulation, and cultured microvascular endothelial cells, demonstrated that the C5a/C5aR1 axis is a key player in endothelial thromboresistance loss. C5a added to normal human serum fully recapitulated the prothrombotic effects of aHUS serum. Mechanistic studies showed that C5a caused RalA-mediated exocytosis of von Willebrand factor (vWF) and P-selectin from Weibel-Palade bodies, which favored further vWF binding on the endothelium and platelet adhesion and aggregation. In patients with severe COVID-19 who suffered from acute activation of complement triggered by severe acute respiratory syndrome coronavirus 2 infection, we found the same C5a-dependent pathogenic mechanisms. These results highlight C5a/C5aR1 as a common prothrombogenic effector spanning from genetic rare diseases to viral infections, and it may have clinical implications. Selective C5a/C5aR1 blockade could have advantages over C5 inhibition because the former preserves the formation of C5b-9, which is critical for controlling bacterial infections that often develop as comorbidities in severely ill patients. The ACCESS trial registered at www.clinicaltrials.gov as #NCT02464891 accounts for the results related to aHUS patients treated with CCX168.
PIAS3 suppresses damage in an Alzheimer’s disease cell model by inducing the STAT3-associated STAT3/Nestin/Nrf2/HO-1 pathway
Authors: Li Chen , Wang Ruili , Zhang Youyou , Hu Chunting , Ma Qiaoya
Published: November 2021

Background

Alzheimer’s disease (AD), the most common form of dementia, is caused by the degeneration of the central nervous system (CNS). A previous study reported that signal transducer and activator of transcription 3 (STAT3) is activated during AD development; nonetheless, the related mechanism remains unknown. Thus, this study used a cell model to explore whether and how the protein inhibitor of activated STAT3 (PIAS3) is involved in AD development.

Methods

Cerebrospinal fluid (CSF) specimens of 30 patients with AD and 10 normal participants were included in this study. SH-SY5Y cells were used to constructed AD model. Relevant indices were then detected and analyzed.

Results

The results showed that compared with the control group, PIAS3 expression was substantially decreased in patients with AD and amyloid beta (Aβ)-treated SH-SY5Y cells. PIAS3 overexpression was able to reverse the detrimental effects of Aβ treatment on cell survival and growth. Further, it could also ameliorate apoptosis and oxidative stress in Aβ-treated SH-SY5Y cells. Additionally, PIAS3 was shown to reduce the activated form of STAT3 and increase the activity of the downstream Nestin/nuclear factor erythroid 2-related factor/heme oxygenase-1 pathway.

Conclusions

STAT3 reactivation by colivelin treatment negated the influence of PIAS3 on the survival, growth, apoptosis, and oxidative stress of Aβ-treated SH-SY5Y cells.
LAGE3 promoted cell proliferation, migration, and invasion and inhibited cell apoptosis of hepatocellular carcinoma by facilitating the JNK and ERK signaling pathway
Authors: Xing Ying , Liu Yang , Qi Zhong , Liu Zhengrong , Wang Xin , Zhang Hongyi
Published: November 2021

Background

Hepatocellular carcinoma (HCC) is now the second leading cause of cancer death worldwide and lacks effectual therapy due to its high rate of tumor recurrence and metastasis. The aim of this study was to investigate the effects of L antigen family member 3 (LAGE3, a member of the LAGE gene family involved in positive transcription) on the progression of HCC.

Methods

The expression of LAGE3 was detected by quantitative real-time polymerase chain reaction. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, colony formation assay, EdU, and cell cycle analysis assay were employed to evaluate the proliferation of HCC cells. Annexin V-FITC/PI and TUNEL assay were used to assess the apoptosis rate of HCC cells. Wound healing and transwell assay were used to investigate the migration and invasion of HCC cells. A xenograft model of HCC was established with 2 × 106 Hep3B or SK-HEP1 cells to investigate the in vivo effects of LAGE3. Then, the protein levels of LAGE3, p-p38, p-38, c-Jun N-terminal kinase (JNK),p-JNK, extracellular signal-regulated kinase (ERK), and p-ERK were detected by western blot.

Results

We found that LAGE3 was upregulated in HCC tissues compared to adjacent tissues, and its high expression was correlated with poor overall survival by bioinformatics analysis. Next, we manually regulated the expression of LAGE3 in HCC cells. The knockdown of LAGE3 inhibited the proliferation of HCC cells by arresting the cell cycle in G1 phase. Also the downregulation of LAGE3 inhibited cell migration and invasion and induced apoptosis of HCC cells, while overexpression of LAGE3 promoted the malignant phenotypes of HCC. These results were further confirmed by the in vivo growth of HCC xenografts and the inhibition of apoptosis of HCC tumor cells. Furthermore, we found that LAGE3 exerted cancer-promoting effects by potentiating the JNK and ERK signaling pathway. An ERK inhibitor (10 μM SCH772984) or JNK inhibitor (25 μM SP600125) repressed the upregulated LAGE3-induced proliferation, migration, and invasion of HCC cells.

Conclusions

LAGE3 enhanced the malignant phenotypes of HCC by promoting the JNK and ERK signaling pathway.
Advances and Challenges of Fluorescent Nanomaterials for Synthesis and Biomedical Applications
Authors: Xiao Deli , Qi Haixiang , Teng Yan , Pierre Dramou , Kutoka Perpetua Takunda , Liu Dong
Published: November 2021

With the rapid development of nanotechnology, new types of fluorescent nanomaterials (FNMs) have been springing up in the past two decades. The nanometer scale endows FNMs with unique optical properties which play a critical role in their applications in bioimaging and fluorescence-dependent detections. However, since low selectivity as well as low photoluminescence efficiency of fluorescent nanomaterials hinders their applications in imaging and detection to some extent, scientists are still in search of synthesizing new FNMs with better properties. In this review, a variety of fluorescent nanoparticles are summarized including semiconductor quantum dots, carbon dots, carbon nanoparticles, carbon nanotubes, graphene-based nanomaterials, noble metal nanoparticles, silica nanoparticles, phosphors and organic frameworks. We highlight the recent advances of the latest developments in the synthesis of FNMs and their applications in the biomedical field in recent years. Furthermore, the main theories, methods, and limitations of the synthesis and applications of FNMs have been reviewed and discussed. In addition, challenges in synthesis and biomedical applications are systematically summarized as well. The future directions and perspectives of FNMs in clinical applications are also presented.
A tool (SPOLERC) to guide the evaluation of phosphorus leaching for agricultural soil by using the change point value in the Xingkai Lake Basin, China
Authors: Xie Zijian , Zhang Fan , Ye Chun , Wang Hao , Wei Weiwei , Li Chunhua , Shi Xiaogang
Published: November 2021

Background

The soil P leaching change point (CP) has been widely used to evaluate soil P leaching risk. However, an automation calculation method for soil P leaching CP value, and an effective risk grading method performed for classifying soil P leaching risk evaluation have not been developed.

Results

This study optimized the calculation process for soil P leaching CP value with two different fitting models. Subsequently, based on the Python programming language, a computation tool named Soil Phosphorus Leaching Risk Calculator (SPOLERC) was developed for soil P leaching risk assessment. SPOLERC not only embedded the calculation process of the soil P leaching CP value, but also introduced the single factor index (SFI) method to grade the soil P leaching risk level. The relationships between the soil Olsen-P and leachable P were fitted by using SPOLERC in paddy soils and arid agricultural soils in the Xingkai Lake Basin, and the results showed that there was a good linear fitting relationship between the soil Olsen-P and leachable P; and the CP values were 59.63 and 35.35 mg Olsen-P kg−1 for paddy soils and arid agricultural soils, respectively. Additionally, 32.7, 21.8, and 3.64% of arid agricultural soil samples were at low risk, medium risk, and high risk of P leaching, and 40.6% of paddy soil samples were at low risk.

Conclusions

SPOLERC can accurately fit the split-line model relationship between the soil Olsen-P and leachable P, and greatly improved the calculation efficiency for the soil P leaching CP value. Additionally, the obtained CP value can be used for soil P leaching risk assessment, which could help recognize key area of soil P leaching.
Differences in olfactory habituation between orthonasal and retronasal pathways
Authors: Xiao Wei , Sun Zhifu , Yan Xiaoguang , Gao Xing , Lv Qianwen , Wei Yongxiang
Published: November 2021

The odorant arrives at nasal olfactory epithelium ortho- and retronasally. This experiment aimed to study the potential different olfactory habituation in orthonasal and retronasal pathways. 68 subjects were stimulated by constant airflow with an odor (50% phenethyl alcohol, PEA or 5% n-butyl acetate, BA) presented ortho- or retronasally. Participants rated the perceived odor intensity (0–10 points) per minute until the odor sensation disappeared. We also investigated the cross-habituation: when the subjects achieved full habituation, continue to rate odor intensity in a different pathway after instantly switching the odor stimulation pathway. The olfactory habituation curve was drawn. The differences of ratings between the orthonasal and retronasal olfaction at different time points and between male and female subjects were analyzed. The two odor intensity ratings decreased as the time extended, share the same “fast followed by slow” type. The ratings of orthonasal olfaction decreased faster than that of retronasal. The intensity rating of PEA of male retronasal approach was lower than that of female at the 5th min (p = 0.018). When orthonasal full habituation achieved, there was significant difference between the intensity ratings and the initial ratings of the retronasal stimulation pathway (p < 0.0001), and vice versa. We found obvious habituation as well as cross-habituation in both orthonasal and retronasal olfaction. The habituation of orthonasal olfaction was faster than that of retronasal olfaction. These different habituations were related to the gender.
Synergistic action of curcumin and cisplatin on spinel ferrite/hierarchical MCM-41 nanocomposite against MCF-7, HeLa and HCT 116 cancer cell line
Authors: Jermy B. Rabindran , Almohazey D. , Alamoudi W. A. , Palanivel R. M. , AlSudairi Nora , Dafalla H. , Almofleh A. A. , Alfareed T. M. , Ravinayagam Vijaya
Published: November 2021

Background

Platinum-based drugs are widely used in cancer therapy, but are known for toxic side effects and resistance. Combinational drug delivery represents an effective chemotherapeutic strategy, but often leads to an increased toxicity. Aim of this study is to test the co-delivery of cisplatin with natural antioxidants on hierarchial porous large surface area hexagonal nanocarriers for synergistic action.

Results

A series of structured mesoporous materials were impregnated with magnetic spinel ferrite (30% CuFe2O4) and then coated with curcumin (25% wt/wt). Mesosilicalite and MCM-41 with high curcumin release abilities were functionalized with cisplatin (5% wt/wt) for synergistic effect of combinational drugs. The cytotoxic efficiency of our nanocomposites was tested on cell viability of MCF7 (human breast cancer), human cervical cancer (HeLa), colorectal cancer (HCT116), and HFF (human foreskin fibroblasts) cell lines using the MTT cell viability assay. At a concentration of 0.1 mg/ml, CuFe2O4/mesosilicalite/curcumin/cisplatin resulted in 89.53% reduction in viability in MCF7, 94.03% in HeLa, 64% in HCT116 and 87% in HFF; whereas, CuFe2O4/MCM-41/curcumin/cisplatin resulted in 76% reduction in viability in MCF7, 64.46% in HeLa, 64% in HCT116 and 24% in HFF. The EC50for CuFe2O4/mesosilicalite/curcumin/cisplatin was 81.23 µg/ml in MCF7, 47.55 µg/ml in HeLa, 48.96 µg/ml in HCT116 and 76.83 µg/ml in HFF. The EC50for CuFe2O4/MCM-41/curcumin/cisplatin was 72.51 µg/ml in MCF7, 58.6 µg/ml in HeLa, 62.58 µg/ml in HCT116 and 154.2 µg/ml in HFF. Furthermore, cells treated with both nanocomposites had a high number of cleaved Caspase 3-positive cells suggesting that the reduction in cell viability was triggered by activating the apoptotic signaling pathway.

Conclusion

Our results show that CuFe2O4/MCM-41/curcumin/cisplatin is a better candidate for combinational drug therapy due to its lowest EC50value and the wider difference in EC50(a fold change) between cancerous and non-cancerous cell line.
Predictive modeling of gene expression regulation
Authors: Regondi Chiara , Fratelli Maddalena , Damia Giovanna , Guffanti Federica , Ganzinelli Monica , Matteucci Matteo , Masseroli Marco
Published: November 2021

Background

In-depth analysis of regulation networks of genes aberrantly expressed in cancer is essential for better understanding tumors and identifying key genes that could be therapeutically targeted.

Results

We developed a quantitative analysis approach to investigate the main biological relationships among different regulatory elements and target genes; we applied it to Ovarian Serous Cystadenocarcinoma and 177 target genes belonging to three main pathways (DNA REPAIR, STEM CELLS and GLUCOSE METABOLISM) relevant for this tumor. Combining data from ENCODE and TCGA datasets, we built a predictive linear model for the regulation of each target gene, assessing the relationships between its expression, promoter methylation, expression of genes in the same or in the other pathways and of putative transcription factors. We proved the reliability and significance of our approach in a similar tumor type (basal-like Breast cancer) and using a different existing algorithm (ARACNe), and we obtained experimental confirmations on potentially interesting results.

Conclusions

The analysis of the proposed models allowed disclosing the relations between a gene and its related biological processes, the interconnections between the different gene sets, and the evaluation of the relevant regulatory elements at single gene level. This led to the identification of already known regulators and/or gene correlations and to unveil a set of still unknown and potentially interesting biological relationships for their pharmacological and clinical use.
Clinical characteristics and prognosis in patients with urosepsis from intensive care unit in Shanghai, China: a retrospective bi-centre study
Authors: Sheng Ying , Zheng Wen-long , Shi Qi-fang , Zhang Bing-yu , Yang Guang-yao
Published: November 2021

Background

The purpose of this study was to retrospectively analyze clinical characteristics and prognostic risk factors of urosepsis patients admitted to two intensive care units in Shanghai, China.

Methods

Clinical data from patients diagnosed with urosepsis were retrospectively retrieved and analyzed from ICU in two regional medical centers from January 2015 to December 2019.

Results

Two hundred two patients were included in the subsequent analysis eventually, with an average age of 72.02 ± 9.66 years, 79.21% of the patients were female and the mortality rate of 15.84%.The proportion of patients with chronic underlying diseases such as diabetes and hypertension was relatively high (56.44, 49.50%, respectively), and the incidence of shock was also high (41.58%) correspondingly. The most common pathogen isolated was Escherichia coli (79.20%), of which the extended-spectrumβ-lactamases (ESBLs)(+) accounted for 42.57%. In multivariate analysis, the strongest predictors for death were mechanical ventilation (OR 7.260, 95% CI 2.200–23.963; P = 0.001),chronic kidney disease (CKD) (OR 5.140, 95% CI 1.596–16.550; P = 0.006), APACHE II score (OR 1.321, 95% CI 1.184–1.473; P < 0.001) and lactate (OR 1.258, 95% CI 1.037–1.527; P = 0.020). Both APACHE II score and lactate had the ideal predictive value, with the area under the ROC curve (AUC) of 0.858 and 0.805 respectively.

Conclusion

The patients with urosepsis were characterized by a higher proportion of female, older age, more percentage of comorbidities in this region, and patients with ESBLs (+) Escherichia coli infection were more prone to shock. Mechanical ventilation, comorbidity with CKD, APACHE II score and lactate were independent risk factors for death in urosepsis patient, but lactate level and APACHE II score had better predictive value for prognosis.
Similarities, reliability and gaps in assessing the quality of conduct of systematic reviews using AMSTAR-2 and ROBIS: systematic survey of nutrition reviews
Authors: Swierz Mateusz J. , Storman Dawid , Zajac Joanna , Koperny Magdalena , Weglarz Paulina , Staskiewicz Wojciech , Gorecka Magdalena , Skuza Anna , Wach Adam , Kaluzinska Klaudia , Bochenek-Cibor Justyna , Johnston Bradley C. , Bala Malgorzata M.
Published: November 2021

Background

AMSTAR-2 (‘A Measurement Tool to Assess Systematic Reviews, version 2’) and ROBIS (‘Risk of Bias in Systematic Reviews’) are independent instruments used to assess the quality of conduct of systematic reviews/meta-analyses (SR/MAs). The degree of overlap in methodological constructs together with the reliability and any methodological gaps have not been systematically assessed and summarized in the field of nutrition.

Methods

We performed a systematic survey of MEDLINE, EMBASE, and the Cochrane Library for SR/MAs published between January 2010 and November 2018 that examined the effects of any nutritional intervention/exposure for cancer prevention. We followed a systematic review approach including two independent reviewers at each step of the process. For AMSTAR-2 (16 items) and ROBIS (21 items), we assessed the similarities, the inter-rater reliability (IRR) and any methodological limitations of the instruments. Our protocol for the survey was registered in PROSPERO (CRD42019121116).

Results

We found 4 similar domain constructs based on 11 comparisons from a total of 12 AMSTAR-2 and 14 ROBIS items. Ten comparisons were considered fully overlapping. Based on Gwet’s agreement coefficients, six comparisons provided almost perfect (> 0.8), three substantial (> 0.6), and one a moderate level of agreement (> 0.4). While there is considerable overlap in constructs, AMSTAR-2 uniquely addresses explaining the selection of study designs for inclusion, reporting on excluded studies with justification, sources of funding of primary studies, and reviewers’ conflict of interest. By contrast, ROBIS uniquely addresses appropriateness and restrictions within eligibility criteria, reducing risk of error in risk of bias (RoB) assessments, completeness of data extracted for analyses, the inclusion of all necessary studies for analyses, and adherence to predefined analysis plan.

Conclusions

Among the questions on AMSTAR-2 and ROBIS, 70.3% (26/37 items) address the same or similar methodological constructs. While the IRR of these constructs was moderate to perfect, there are unique methodological constructs that each instrument independently addresses. Notably, both instruments do not address the reporting of absolute estimates of effect or the overall certainty of the evidence, items that are crucial for users’ wishing to interpret the importance of SR/MA results.
Comparing current and emerging practice models for the extrapolation of survival data: a simulation study and case-study
Authors: Kearns Benjamin , Stevenson Matt D. , Triantafyllopoulos Kostas , Manca Andrea
Published: November 2021

Background

Estimates of future survival can be a key evidence source when deciding if a medical treatment should be funded. Current practice is to use standard parametric models for generating extrapolations. Several emerging, more flexible, survival models are available which can provide improved within-sample fit. This study aimed to assess if these emerging practice models also provided improved extrapolations.

Methods

Both a simulation study and a case-study were used to assess the goodness of fit of five classes of survival model. These were: current practice models, Royston Parmar models (RPMs), Fractional polynomials (FPs), Generalised additive models (GAMs), and Dynamic survival models (DSMs). The simulation study used a mixture-Weibull model as the data-generating mechanism with varying lengths of follow-up and sample sizes. The case-study was long-term follow-up of a prostate cancer trial. For both studies, models were fit to an early data-cut of the data, and extrapolations compared to the known long-term follow-up.

Results

The emerging practice models provided better within-sample fit than current practice models. For data-rich simulation scenarios (large sample sizes or long follow-up), the GAMs and DSMs provided improved extrapolations compared with current practice. Extrapolations from FPs were always very poor whilst those from RPMs were similar to current practice. With short follow-up all the models struggled to provide useful extrapolations. In the case-study all the models provided very similar estimates, but extrapolations were all poor as no model was able to capture a turning-point during the extrapolated period.

Conclusions

Good within-sample fit does not guarantee good extrapolation performance. Both GAMs and DSMs may be considered as candidate extrapolation models in addition to current practice. Further research into when these flexible models are most useful, and the role of external evidence to improve extrapolations is required.
Modular literature review: a novel systematic search and review method to support priority setting in health policy and practice
Authors: Koivu Annariina M. , Hunter Patricia J. , Näsänen-Gilmore Pieta , Muthiani Yvonne , Isojärvi Jaana , Pörtfors Pia , Ashorn Ulla , Ashorn Per
Published: November 2021

Background

There is an unmet need for review methods to support priority-setting, policy-making and strategic planning when a wide variety of interventions from differing disciplines may have the potential to impact a health outcome of interest. This article describes a Modular Literature Review, a novel systematic search and review method that employs systematic search strategies together with a hierarchy-based appraisal and synthesis of the resulting evidence.

Methods

We designed the Modular Review to examine the effects of 43 interventions on a health problem of global significance. Using the PICOS (Population, Intervention, Comparison, Outcome, Study design) framework, we developed a single four-module search template in which population, comparison and outcome modules were the same for each search and the intervention module was different for each of the 43 interventions. A series of literature searches were performed in five databases, followed by screening, extraction and analysis of data. “ES documents”, source documents for effect size (ES) estimates, were systematically identified based on a hierarchy of evidence. The evidence was categorised according to the likely effect on the outcome and presented in a standardised format with quantitative effect estimates, meta-analyses and narrative reporting. We compared the Modular Review to other review methods in health research for its strengths and limitations.

Results

The Modular Review method was used to review the impact of 46 antenatal interventions on four specified birth outcomes within 12 months. A total of 61,279 records were found; 35,244 were screened by title-abstract. Six thousand two hundred seventy-two full articles were reviewed against the inclusion criteria resulting in 365 eligible articles.

Conclusions

The Modular Review preserves principles that have traditionally been important to systematic reviews but can address multiple research questions simultaneously. The result is an accessible, reliable answer to the question of “what works?”. Thus, it is a well-suited literature review method to support prioritisation, decisions and planning to implement an agenda for health improvement.