bluuebunny/crossref_metadata_2025 huggingface dataset:
Fatty Acids and Eicosanoids Change during High-Fiber Diet in NAFLD Patients—Randomized Control Trials (RCT)
Authors: Maciejewska-Markiewicz Dominika , Drozd Arleta , Palma Joanna , Ryterska Karina , Hawryłkowicz Viktoria , Załęska Patrycja , Wunsh Ewa , Kozłowska-Petriczko Katarzyna , Stachowska Ewa
Published: October 2022

Background: Non-alcoholic fatty liver disease (NAFLD) is a wide spectrum condition characterized by excessive liver fat accumulation in people who do not abuse alcohol. There is no effective medical treatment for NAFLD; therefore, most important recommendations to reduce liver steatosis are diet and lifestyle, including proper physical activity. The aim of our study was to analyze the fatty acids and eicosanoids changes in the serum of patients who consumed high-fiber rolls for 8 weeks. Materials and Methods: The group of 28 Caucasian participants was randomly divided into two groups, those who received 24 g of fiber/day—from 2 buns of 12 g each (n = 14), and those who received 12 g of fiber/day—from 2 buns of 6 g (n = 14). At the beginning and on the last visit of the 8-week intervention, all patients underwent NAFLD evaluation, biochemical parameter measurements, and fatty acids and eicosanoids evaluation. Results: Patients who received 12 g of fiber had significantly reduced liver steatosis and body mass index. In the group who received 24 g of fiber/day, we observed a trend to liver steatosis reduction (p = 0.07) and significant decrease in aspartate aminotransferase (p = 0.03) and total cholesterol (p = 0.03). All changes in fatty acid and eicosanoids profile were similar. Fatty acids analysis revealed that extra fiber intake was associated with a significant increase in monounsaturated fatty acids and decrease in saturated fatty acids. Moreover, both groups showed increased concentration of gamma linoleic acid and docosahexaenoic acid. We also observed reduction in prostaglandin E2. Conclusions: Our study revealed that a high amount of fiber in the diet is associated with a reduction in fatty liver, although this effect was more pronounced in patients in the lower fiber group. However, regardless of the amount of fiber consumed, we observed significant changes in the profile of FAs, which may reflect the positive changes in the lipids liver metabolism. Regardless of the amount of fiber consumed, patients decreased the amount of PGE2, which may indicate the lack of disease progression associated with the development of inflammation.
A Program Evaluation of a Dietary Sodium Reduction Research Consortium of Five Low- and Middle-Income Countries in Latin America
Authors: Padilla-Moseley Janice , Blanco-Metzler Adriana , L’Abbé Mary R. L’Abbé , Arcand JoAnne
Published: October 2022

Excess dietary sodium is a global public health priority, particularly in low- and middle-income countries where rates of hypertension and cardiovascular disease are high. The International Development Research Centre funded a research consortium of five Latin American countries (LAC) to inform public health policy for dietary sodium reduction (2016–2020). The objective of this study was to determine the outcomes of this funding on short-term (e.g., research, capacity building) and intermediary outcomes (e.g., policies). A summative program evaluation was conducted, using a logic model and multiple data sources including document review, surveys and interviews. Researchers from Argentina, Costa Rica, Brazil, Peru and Paraguay produced a significant amount of scientific evidence to guide decision making on sodium policy related to its content in foods, consumer behaviors (social marketing), and the health and economic benefits of dietary reduction. A substantive number of knowledge translation products were produced. The funding enabled training opportunities for researchers who developed skills that can be scaled-up to other critical nutrients and health issues. It was unexpected that intermediary policy changes would occur, however several countries demonstrated early policy improvements derived from this research. A funded research consortium of LAC is a practical approach to invoke policy innovations.
Hearing Function and Nutritional Status in Aviation Pilots from Spain Exposed to High Acoustic Damage
Authors: Morais-Moreno Carmen , Montero-Bravo Ana M. , Puga Ana M. , de Lourdes Samaniego-Vaesken Mª , Ruperto Mar , Marco Mendez Rocío , Vicente-Arche Álvaro , Varela-Moreiras Gregorio , Partearroyo Teresa
Published: October 2022

Noise-induced hearing loss is the most frequent and preventable occupational disease. Aviation pilots are a vulnerable population, as they spend many hours exposed to noise pollution in their working environment. Different studies suggest that certain dietary compounds may play a key role in the etiology and prevention of this pathology. We aimed to study the relationship linking auditory function, dietary intake, and some serum biomarkers in pilots, exposed to noise in the work environment. A total of 235 pilots, who undergo a periodic medical examination at the Centro de Instrucción de Medicina Aeroespacial (Madrid, SPAIN), were evaluated. Auditory function was assessed by tonal audiometry. Energy and nutrient intakes were estimated by 24 h recall (DIALTM program). Serum homocysteine (Hcy) was determined in a Cobas 6000TM multi-analyzer while folate, vitamin B12, and D were analyzed in a Cobas e411TM. The prevalence of hearing loss (HL) was 64.3%. HL was significantly related to age (r = 0.588, p ≤ 0.001) and flight hours (r = 0.283, p ≤ 0.001). A multiple linear regression model was used to assess the relationship among HL, flight hours, serum folate, and Hcy serum levels. HL was significantly (p < 0.050) associated with flight hours (β = 0.246), serum folate (β = −0.143), and serum Hcy (β = 0.227). Nutritional interventions would be of great interest to monitor and slow down the HL progression in populations exposed to noise pollution in their workplace.
Branched-Chain Amino Acids Supplementation Does Not Accelerate Recovery after a Change of Direction Sprinting Exercise Protocol
Authors: Khemtong Chutimon , Tessitore Antonio , Jaime Salvador J. , Gobbi Giuliana , Jensen Jørgen , Yang Ai-Lun , Kuo Chia-Hua , Condello Giancarlo
Published: October 2022

BCAAs supplementation has been widely used for post-exercise recovery. However, no evidence is currently available to answer the question of whether BCAAs supplementation can attenuate muscle damage and ameliorate recovery after a bout of change of direction (COD) sprinting, which is an exercise motion frequently used during team sport actions. This study aimed to assess the effect of BCAAs supplementation on muscle damage markers, subjective muscle soreness, neuromuscular performance, and the vascular health of collegiate basketball players during a 72 h recovery period after a standardized COD protocol. Participants orally received either BCAAs (0.17 g/kg BCAAs + 0.17 g/kg glucose) or placebo (0.34 g/kg glucose) supplementation before and immediately after a COD exercise protocol in a randomized, crossover, double-blind, and placebo-controlled manner. Creatine kinase increased immediately after exercise and peaked at 24 h, muscle soreness remained elevated until 72 h, whilst arterial stiffness decreased after exercise for both supplemented conditions. A negligibly lower level of interleukin-6 was found in the BCAAs supplemented condition. In conclusion, the results of this study do not support the benefits of BCAAs supplementation on mitigating muscle damage and soreness, neuromuscular performance, and arterial stiffness after COD for basketball players.
Cyclic VLCKD Meal Replacement in a Patient with Obesity and Mild Chronic Kidney Disease following Kidney Transplantation
Authors: Vigna Luisella , Tomaino Laura , Lotito Veronica , Ingenito Maria Rosaria , Piontini Alessandra , Marsili Alessandro
Published: October 2022

(1) Background: this work reports the clinical case of a man who underwent a kidney transplant at the age of 19 for idiopathic interstitial nephritis resulting in renal failure. (2) Methods: the patient also had severe obesity and underwent four cycles of a very low-calorie ketogenic diet (VLCKD) for weight loss. (3) Results: the cycles of diet therapy carried out during a six-year period allowed a significant weight loss (from 103.2 kg in 2012 to 87.5 kg in 2018) with lean mass conservation (from 49.9 kg to 52.5 kg) and a reduction of fat mass (from 53.3 kg to 35.0 kg), as assessed with bioimpedance analysis. Throughout the diet cycles, body weight remained stable, except for a weight regain that occurred between the 2nd and 3rd cycle. Renal function was preserved despite the known mild chronic kidney disease (creatininemia ranged from 1.11 mg/dL to 1.27 mg/dL, and GRF from 91 to 71 mL/min/1.73 m2). (4) Conclusions: this report shows that a VLCKD, performed under strict medical supervision, is safe and effective even in a complex clinical picture.
Gene Screening in High-Throughput Right-Censored Lung Cancer Data
Authors: Ke Chenlu , Bandyopadhyay Dipankar , Acunzo Mario , Winn Robert
Published: October 2022

Background: Advances in sequencing technologies have allowed collection of massive genome-wide information that substantially advances lung cancer diagnosis and prognosis. Identifying influential markers for clinical endpoints of interest has been an indispensable and critical component of the statistical analysis pipeline. However, classical variable selection methods are not feasible or reliable for high-throughput genetic data. Our objective is to propose a model-free gene screening procedure for high-throughput right-censored data, and to develop a predictive gene signature for lung squamous cell carcinoma (LUSC) with the proposed procedure. Methods: A gene screening procedure was developed based on a recently proposed independence measure. The Cancer Genome Atlas (TCGA) data on LUSC was then studied. The screening procedure was conducted to narrow down the set of influential genes to 378 candidates. A penalized Cox model was then fitted to the reduced set, which further identified a 6-gene signature for LUSC prognosis. The 6-gene signature was validated on datasets from the Gene Expression Omnibus. Results: Both model-fitting and validation results reveal that our method selected influential genes that lead to biologically sensible findings as well as better predictive performance, compared to existing alternatives. According to our multivariable Cox regression analysis, the 6-gene signature was indeed a significant prognostic factor (p-value < 0.001) while controlling for clinical covariates. Conclusions: Gene screening as a fast dimension reduction technique plays an important role in analyzing high-throughput data. The main contribution of this paper is to introduce a fundamental yet pragmatic model-free gene screening approach that aids statistical analysis of right-censored cancer data, and provide a lateral comparison with other available methods in the context of LUSC.
Detection of Theileria orientalis Genotypes from Cattle in Kyrgyzstan
Authors: Ozubek Sezayi , Ulucesme Mehmet Can , Cirak Veli Yılgor , Aktas Munir
Published: October 2022

The ikeda and chitose genotypes of Theileria orientalis, which for many years were thought to be benign, cause a disease that results in significant economic losses in the cattle industry. This study was carried out in order to determine the genotypes of T. orientalis in cattle in Kyrgyzstan, and 149 archived DNA samples known to be T. orientalis were analyzed by the PCR amplification of the major piroplasm surface protein (MPSP) gene region. Single-Strand Conformation Polymorphism (SSCP) analysis was performed to uncover the nucleotide changes in the archived DNA samples, and 15 samples showing different band profiles were subjected to sequence analysis. As a result of the sequence analysis, it was seen that the samples belonged to the buffeli and chitose A genotypes. In order to identify mixed genotypes, PCR was performed using primers specific for these genotypes, and buffeli (type 3), chitose (type 1) and buffeli+chitose were found to be positive in 26.2%, 2% and 71.8% of samples, respectively. As a result of this study, we showed the presence of buffeli (type 3) and chitose (type 1) genotypes of T. orientalis in cattle in Kyrgyzstan. Comprehensive epidemiological studies are needed to understand the clinical infections caused by the pathogenic chitose A and to determine the geographical distribution and different genotypes of T. orientalis.
Pathogenic Bacilli as an Emerging Biothreat?
Authors: Mondange Lou , Tessier Émilie , Tournier Jean-Nicolas
Published: October 2022

Bacillus anthracis, present as a very durable endospore in soil, causes zoonotic illness which is mainly associated with herbivores and domestic animals. Human cases are scarce and often involve populations close to infected livestock. If anthrax is no longer of public health concern in developed countries, B. anthracis is one of the top-tier biological weapon agents. It is classified by the CDC as a category A agent. Since 1994, emerging strains of Bacillus cereus have been associated with anthrax-like disease in mammals. Some clinical strains of B. cereus harbor anthrax-like plasmid genes (pXO1 and pXO2) associated with non-human primate and human infections, with the same clinical presentation of inhalation anthrax and mortality rates. Although currently restricted to certain limited areas of circulation, the emergence of these new strains of B. cereus extends the list of potential agents possibly usable for bioterrorism or as a biological weapon. It is therefore important to improve our knowledge of the phylogeny within the B. cereus sensu lato group to better understand the origin of these strains. We can then more efficiently monitor the emergence of new strains to better control the risk of infection and limit potentially malicious uses.
Long-Term Outcomes in Children with Congenital Toxoplasmosis—A Systematic Review
Authors: Garweg Justus G. , Kieffer François , Mandelbrot Laurent , Peyron François , Wallon Martine
Published: October 2022

Even in the absence of manifestations at birth, children with congenital toxoplasmosis (CT) may develop serious long-term sequelae later in life. This systematic review aims to present the current state of knowledge to base an informed decision on how to optimally manage these pregnancies and children. For this, a systematic literature search was performed on 28 July 2022 in PubMed, CENTRAL, ClinicalTrials.gov, Google Scholar and Scopus to identify all prospective and retrospective studies on congenital toxoplasmosis and its long-term outcomes that were evaluated by the authors. We included 31 research papers from several countries. Virulent parasite strains, low socioeconomic status and any delay of treatment seem to contribute to a worse outcome, whereas an early diagnosis of CT as a consequence of prenatal screening may be beneficial. The rate of ocular lesions in treated children increases over time to 30% in European and over 70% in South American children and can be considerably reduced by early treatment in the first year of life. After treatment, new neurological manifestations are not reported, while ocular recurrences are observed in more than 50% of patients, with a mild to moderate impact on quality of life in European cohorts when compared to a significantly reduced quality of life in the more severely affected South American children. Though CT is rare and less severe in Europe when compared with South America, antenatal screening is the only effective way to diagnose and treat affected individuals at the earliest possible time in order to reduce the burden of disease and achieve satisfying outcomes.
Chemistry and Bioactivities of Six Tunisian Eucalyptus Species
Authors: Kouki Habiba , Polito Flavio , De Martino Laura , Mabrouk Yassine , Hamrouni Lamia , Amri Ismail , Fratianni Florinda , De Feo Vincenzo , Nazzaro Filomena
Published: October 2022

The complex taxonomy of Eucalyptus genus, the renewed interest in natural compounds able to combat microbial strains, the overuse of synthetic pesticides, the consequent request for alternative control methods were the reasons for this research. The essential oils (Eos) of Eucalyptus bosistoana, Eucalyptus melliodora, Eucalyptus odorata, Eucalyptus paniculata, Eucalyptus salmonopholia, and Eucalyptus transcontinentalis were analyzed by GC/MS and their potential phytotoxic activity was evaluated against the germination and radicle elongation of Sinapis arvensis, Raphanus sativus and Lolium multiflorum. The antibiofilm activity was assayed against both Gram-positive (Staphylococcus aureus and Listeria monocytogenes) and Gram-negative (Pseudomonas aeruginosa, Escherichia coli, and Acinetobacter baumannii) bacteria. Monoterpenoids were the most representative constituents in all EOs and eucalyptol was the dominant component except in E. melliodora EO, in which p-cymene was the most abundant. In phytotoxic assays, the EOs from E. odorata and E. paniculata were the most active against germination and radical elongation of the tested seeds. Finally, the Eucalyptus EOs proved their capacity to effectively inhibit the adhesion process of all five pathogen strains, with percentages often reaching and exceeding 90%. These Eucalytpus EOs could have possible employments in the food, health and agricultural fields.
Updates on Responsive Drug Delivery Based on Liposome Vehicles for Cancer Treatment
Authors: Nikolova Maria P. , Kumar Enamala Manoj , Chavali Murthy S.
Published: October 2022

Liposomes are well-known nanoparticles with a non-toxic nature and the ability to incorporate both hydrophilic and hydrophobic drugs simultaneously. As modern drug delivery formulations are produced by emerging technologies, numerous advantages of liposomal drug delivery systems over conventional liposomes or free drug treatment of cancer have been reported. Recently, liposome nanocarriers have exhibited high drug loading capacity, drug protection, improved bioavailability, enhanced intercellular delivery, and better therapeutic effect because of resounding success in targeting delivery. The site targeting of smart responsive liposomes, achieved through changes in their physicochemical and morphological properties, allows for the controlled release of active compounds under certain endogenous or exogenous stimuli. In that way, the multifunctional and stimuli-responsive nanocarriers for the drug delivery of cancer therapeutics enhance the efficacy of treatment prevention and fighting over metastases, while limiting the systemic side effects on healthy tissues and organs. Since liposomes constitute promising nanocarriers for site-targeted and controlled anticancer drug release, this review focuses on the recent progress of smart liposome achievements for anticancer drug delivery applications.
Gold-Polypyrrole-Loaded Eosin in Photo-Mediated Treatment of Hidradenitis Suppurativa: In Vivo Trans-Epidermal Permeation Study and Clinical Case Report
Authors: El-Kholy Abdullah I. , Fadel Maha , Nasr Maha , El-Sherbiny Ibrahim , Tawfik Abeer , Mosaad Yasser O. , Abdel Fadeel Doaa
Published: October 2022

This study reports a new protocol for the management of Hidradenitis Suppurativa (HS), depending on the synergistic photodynamic and photothermal effect of eosin yellow-gold-polypyrrole hybrid nanoparticles (E-G-Ppy NPs). E-G-Ppy NPs and gold-polypyrrole NPs (G-Ppy NPs) were synthesized, characterized, and formulated in topical hydrogels. Then, in vivo trans-epidermal permeation study, under both dark and white light-irradiation conditions, was done on albino mice. The E-G-Ppy hydrogel was then applied on a twenty-four years old female with recurrent axillary HS lesions pretreated with fractional CO2 laser. Thereafter, the treated lesions were irradiated sequentially, using an IPL system, in the visible (~550 nm) and NIR band (630–1100 nm) to activate the synthesized nanoparticles. Results showed that, upon application to mice skin, E-G-Ppy exhibited good tolerance and safety under dark conditions and induced degenerative changes into dermal layers after white-light activation, reflecting deep penetration. Photo-activation of E-G-Ppy hydrogel to a severe Hidradenitis Suppurativa case showed an improvement of 80% of the lesions according to average HS-LASI scores after 4 sessions with no recurrence during a follow-up period of six months. In summary, the dual photodynamic/photothermal activation of E-G-Ppy NPs can represent a promising modality for management of HS. Further expanded clinical studies may be needed.
Polymers Enhancing Bioavailability in Drug Delivery
Authors: Fernandes Ana I. , Jozala Angela F.
Published: October 2022

A drug’s bioavailability, i.e., the extent to and rate at which it enters the systemic circulation, thus accessing the site of action, is largely determined by the properties of the drug [...]
Efficacy and Safety of a Brain-Penetrant Biologic TNF-α Inhibitor in Aged APP/PS1 Mice
Authors: Ou Weijun , Ohno Yuu , Yang Joshua , Chandrashekar Devaraj V. , Abdullah Tamara , Sun Jiahong , Murphy Riley , Roules Chuli , Jagadeesan Nataraj , Cribbs David H. , Sumbria Rachita K.
Published: October 2022

Tumor necrosis factor alpha (TNF-α) plays a vital role in Alzheimer’s disease (AD) pathology, and TNF-α inhibitors (TNFIs) modulate AD pathology. We fused the TNF-α receptor (TNFR), a biologic TNFI that sequesters TNF-α, to a transferrin receptor antibody (TfRMAb) to deliver the TNFI into the brain across the blood–brain barrier (BBB). TfRMAb-TNFR was protective in 6-month-old transgenic APP/PS1 mice in our previous work. However, the effects and safety following delayed chronic TfRMAb-TNFR treatment are unknown. Herein, we initiated the treatment when the male APP/PS1 mice were 10.7 months old (delayed treatment). Mice were injected intraperitoneally with saline, TfRMAb-TNFR, etanercept (non-BBB-penetrating TNFI), or TfRMAb for ten weeks. Biologic TNFIs did not alter hematology indices or tissue iron homeostasis; however, TfRMAb altered hematology indices, increased splenic iron transporter expression, and increased spleen and liver iron. TfRMAb-TNFR and etanercept reduced brain insoluble-amyloid beta (Aβ) 1-42, soluble-oligomeric Aβ, and microgliosis; however, only TfRMAb-TNFR reduced Aβ peptides, Thioflavin-S-positive Aβ plaques, and insoluble-oligomeric Aβ and increased plaque-associated phagocytic microglia. Accordingly, TfRMAb-TNFR improved spatial reference memory and increased BBB-tight junction protein expression, whereas etanercept did not. Overall, despite delayed treatment, TfRMAb-TNFR resulted in a better therapeutic response than etanercept without any TfRMAb-related hematology- or iron-dysregulation in aged APP/PS1 mice.
Recent Advances in Amorphous Solid Dispersions: Preformulation, Formulation Strategies, Technological Advancements and Characterization
Authors: Tambe Srushti , Jain Divya , Meruva Sai Kishore , Rongala Gopinath , Juluri Abhishek , Nihalani Girish , Mamidi Hemanth Kumar , Nukala Pavan Kumar , Bolla Pradeep Kumar
Published: October 2022

Amorphous solid dispersions (ASDs) are among the most popular and widely studied solubility enhancement techniques. Since their inception in the early 1960s, the formulation development of ASDs has undergone tremendous progress. For instance, the method of preparing ASDs evolved from solvent-based approaches to solvent-free methods such as hot melt extrusion and Kinetisol®. The formulation approaches have advanced from employing a single polymeric carrier to multiple carriers with plasticizers to improve the stability and performance of ASDs. Major excipient manufacturers recognized the potential of ASDs and began introducing specialty excipients ideal for formulating ASDs. In addition to traditional techniques such as differential scanning calorimeter (DSC) and X-ray crystallography, recent innovations such as nano-tomography, transmission electron microscopy (TEM), atomic force microscopy (AFM), and X-ray microscopy support a better understanding of the microstructure of ASDs. The purpose of this review is to highlight the recent advancements in the field of ASDs with respect to formulation approaches, methods of preparation, and advanced characterization techniques
Far-Red Light Effects on Lettuce Growth and Morphology in Indoor Production Are Cultivar Specific
Authors: Liu Jun , van Iersel Marc W.
Published: October 2022

Understanding crop responses to the light spectrum is critical for optimal indoor production. Far-red light is of particular interest, because it can accelerate growth through both physiological and morphological mechanisms. However, the optimal amount of supplemental far-red light for indoor lettuce production is yet to be quantified. Lettuce ‘Cherokee’, ‘Green SaladBowl’, and ‘Little Gem’ were grown under 204 µmol·m−2·s−1 warm-white light-emitting diodes (LEDs) with supplemental far-red ranging from 5.3 to 75.9 µmol·m−2·s−1. Supplemental far-red light increased canopy light interception 5 days after the start of far-red light treatment (DAT) for ‘Green SaladBowl’ and ‘Little Gem’ and 7 DAT for ‘Cherokee’. The increase in light interception was no longer evident after 12 and 16 DAT for ‘Green SaladBowl’ and ‘Little Gem’, respectively. We did not find evidence that supplemental far-red light increased leaf-level photosynthesis. At the final harvest, shoot dry weights of ‘Cherokee’ and ‘Little Gem’ increased by 39.4% and 19.0%, respectively, while ‘Green SaladBowl’ was not affected. In conclusion, adding far-red light in indoor production increased light interception during early growth and likely increased whole plant photosynthesis thus growth, but those effects were cultivar-specific; the increase in dry weight was linear up to 75.9 µmol·m−2·s−1 far-red light.
Targeted Whole Genome Sequencing (TWG-Seq) of Cucumber Green Mottle Mosaic Virus Using Tiled Amplicon Multiplex PCR and Nanopore Sequencing
Authors: Mackie Joanne , Kinoti Wycliff M. , Chahal Sumit I. , Lovelock David A. , Campbell Paul R. , Tran-Nguyen Lucy T. T. , Rodoni Brendan C. , Constable Fiona E.
Published: October 2022

Rapid and reliable detection tools are essential for disease surveillance and outbreak management, and genomic data is essential to determining pathogen origin and monitoring of transmission pathways. Low virus copy number and poor RNA quality can present challenges for genomic sequencing of plant viruses, but this can be overcome by enrichment of target nucleic acid. A targeted whole genome sequencing (TWG-Seq) approach for the detection of cucumber green mottle mosaic virus (CGMMV) has been developed where overlapping amplicons generated using two multiplex RT-PCR assays are then sequenced using the Oxford Nanopore MinION. Near complete coding region sequences were assembled with ≥100× coverage for infected leaf tissue dilution samples with RT-qPCR cycle quantification (Cq) values from 11.8 to 38 and in seed dilution samples with Cq values 13.8 to 27. Consensus sequences assembled using this approach showed greater than 99% nucleotide similarity when compared to genomes produced using metagenomic sequencing. CGMMV could be confidently detected in historical seed isolates with degraded RNA. Whilst limited access to, and costs associated with second-generation sequencing platforms can influence diagnostic outputs, the portable Nanopore technology offers an affordable high throughput sequencing alternative when combined with TWG-Seq for low copy or degraded samples.
Evaluation of the Phytochemical Composition of Phenolic and Triterpene Compounds in Fruit of Large Cranberries (Vaccinium macrocarpon Aiton) Grown in Latvia
Authors: Šedbarė Rima , Siliņa Dace , Janulis Valdimaras
Published: October 2022

We carried out a qualitative and quantitative analysis of the phytochemical composition of the fruits of large cranberry cultivars ‘Ben Lear’, ‘Bergman’, ‘Kalnciema agra’, ‘Lemunyon’, ‘Pilgrim’, ‘Stevens’, and ‘Tina’ grown in Latvian climatic conditions. The following predominant compounds were found in cranberry fruit samples: peonidin-3-O-galactoside, peonidin-3-O-arabinoside, cyanidin-3-O-galactoside, cyanidin-3-O-arabinoside, myricetin-3-galactoside, quercetin-3-galactoside, quercetin-3-α-L-arabinofuranoside, quercetin 3-rhamnoside, ursolic acid, and oleanolic acid. During the berry ripening period (from 16 August until 15 September), a trend of decreasing amounts of compounds was found in the fruit samples of the studied cranberry cultivars: the total amount of proanthocyanidins decreased by 1.3 times, the total amount of the identified flavonols decreased by 1.3 times, the total amount of triterpenoids decreased by 1.2 times, and the total amount of chlorogenic acid decreased by 1.7 times. During the period from 16 August until 15 September, the total amount of anthocyanins in the cranberry fruit samples increased by 2.6 to 17 times. The highest total amount of anthocyanins (5305.80 ± 27 µg/g) was detected in fruit samples of the cranberry cultivar ‘Kalnciema agra’ collected on 15 September. The amount of biologically active compounds in cranberry fruit samples varies during berry ripening. Thus, the choice of the picking time is one of the factors that determines the phytochemical composition of raw cranberry material.
Effects of Plant-Growth-Promoting Rhizobacteria (PGPR) and Cyanobacteria on Botanical Characteristics of Tomato (Solanum lycopersicon L.) Plants
Authors: Gashash Ebtesam A. , Osman Nahid A. , Alsahli Abdulaziz A. , Hewait Heba M. , Ashmawi Ashmawi E. , Alshallash Khalid S. , El-Taher Ahmed M. , Azab Enas S. , Abd El-Raouf Hany S. , Ibrahim Mohamed F. M.
Published: October 2022

Tomatoes are an important agricultural product because they contain high concentrations of bioactive substances, such as folate, ascorbate, polyphenols, and carotenoids, as well as many other essential elements. As a result, tomatoes are thought to be extremely beneficial to human health. Chemical fertilizers and insecticides are routinely utilized to maximize tomato production. In this context, microbial inoculations, particularly those containing PGPR, may be utilized in place of chemical fertilizers and pesticides. In this study, we investigated the effects of PGPR (Bacillus subtilis, and Bacillus amyloliquefaciens) and cyanobacteria when utilized alone, and in conjunction with each other, on the growth, quality, and yield of fresh fruits of tomato plants. The results showed that the inoculation significantly increased all measured parameters of tomato plants compared with the control. Combined use of B. subtilis and B. amyloliquefaciens had a positive impact on tomato yield, increasing fruit yield. Moreover, leaflet anatomical characteristics were altered, with increased thickness of the upper epidermis, lower epidermis, palisade tissue, spongy tissue, and vascular bundles. Tomato fruit quality was improved, as measured by an increased number of fruit per plant (76% increase), fruit weight (g; 33% increase), fruit height (cm; 50% increase), fruit diameter (cm; 50%), total soluble solids (TSS; 26% increase), and ascorbic acid (mg/100 g F.W.; 75% increase), relative to the control, in the first season. In addition, fruit chemical contents (N, P, and K) were increased with inoculation. The results suggest that inoculation with B. subtilis and B. amyloliquefaciens could be successfully used to enhance tomato plant growth and yield.
Long−Distance Wind Dispersal Drives Population Range Expansion of Solidago canadensis
Authors: Zhang Zheng , Wen Guangyue , Bu Dexiao , Sun Guojun , Qiang Sheng
Published: October 2022

Canada goldenrod (Solidago canadensis L.) is a serious invasive alien plant species that exerts negative effects on natural and agricultural ecosystems in China. Few studies have addressed the dispersal of S. canadensis to explain how it rapidly spreads to large areas over long distances. Here, we quantify the dispersal of S. canadensis via wind by capturing in situ−stained diaspores. The diaspores were trapped and counted along 11 radiating transects from the center of a dispersal source. Solidago canadensis diaspores could be dispersed in all directions from the source, traveling longer distances and in greater amounts in the downwind direction than the upwind one. With a source including about 58 million diaspores and a wind speed at Beaufort scale 4, the dispersal distance in the prevailing wind direction (PWD) was at least 2000 m. Diaspores shattered at a rate of approximately 3% daily with the common wind speed of scale 4, indicating that dispersal could last for more than a month. A mechanistic model was used to fit the dispersal curve along the PWD. Although the model slightly underestimated long−distance dispersal, it still demonstrated the potential of long−distance dispersal with great source strength. Wind−dispersed diaspores to new areas persisted over winter and were able to form new plants at a density of about 2 plants per m2 at 500 m away from the source. Further experiments showed that the dispersed amount of S. canadensis diaspores was significantly positively correlated with the temperature and wind speed, but significantly negatively correlated with relative humidity, which indicated that, during a day, the maximum dispersal usually occurred in the afternoon when the temperature was the highest and the relative humidity the lowest. In addition, for an already existent population patch, the patch range can expand 2−4 m per year, mainly depending on the seedlings recruited from the rhizomes. These results provide insights into the long−distance dispersal of S. canadensis by wind and its effects on the range expansion process.
Tailored Polyelectrolyte Multilayer Systems by Variation of Polyelectrolyte Composition and EDC/NHS Cross-Linking: Controlled Drug Release vs. Drug Reservoir Capabilities and Cellular Response for Improved Osseointegration
Authors: Ludolph Johanna , Rothe Holger , Schirmer Uwe , Möbus Katharina , Behrens Christina , Schliephake Henning , Liefeith Klaus
Published: October 2022

Polyelectrolyte multilayers (PEM) are versatile tools used to investigate fundamental interactions between material-related parameters and the resulting performance in stem cell differentiation, respectively, in bone tissue engineering. In the present study, we investigate the suitability of PEMs with a varying collagen content for use as drug carriers for the human bone morphogenetic protein 2 (rhBMP-2). We use three different PEM systems consisting either of the positively charged poly-L-lysine or the glycoprotein collagen type I and the negatively charged glycosaminoglycan heparin. For a specific modification of the loading capacity and the release kinetics, the PEMs were stepwise cross-linked before loading with cytokine. We demonstrate the possibility of immobilizing significant amounts of rhBMP-2 in all multilayer systems and to specifically tune its release via cross-linking. Furthermore, we prove that the drug release of rhBMP-2 plays only a minor role in the differentiation of osteoprogenitor cells. We find a significantly higher influence of the immobilized rhBMP-2 within the collagen-rich coatings that obviously represent an excellent mimicry of the native extracellular matrix. The cytokine immobilized in its bioactive form was able to achieve an increase in orders of magnitude both in the early stages of differentiation and in late calcification compared to the unloaded layers.
Synergistic Membrane Disturbance Improves the Antibacterial Performance of Polymyxin B
Authors: Li Wenwen , Zhang Che , Lu Xuemei , Sun Shuqing , Yang Kai , Yuan Bing
Published: October 2022

Drug-resistant Gram-negative bacteria pose a serious threat to public health, and polymyxin B (PMB) is clinically used as a last-line therapy for the treatment of infections caused by these pathogens. However, the appearance of PMB resistance calls for an effort to develop new approaches to improve its antibacterial performance. In this work, a new type of nanocomposite, composed of PMB molecules being chemically decorated on the surface of graphene oxide (GO) nanosheets, was designed, which showed potent antibacterial ability through synergistically and physically disturbing the bacterial membrane. The as-fabricated PMB@GO nanocomposites demonstrated an enhanced bacterial-killing efficiency, with a minimum inhibitory concentration (MIC) value half of that of free PMB (with an MIC value as low as 0.5 μg mL−1 over Escherichia coli), and a bacterial viability less than one fourth of that of PMB (with a bacterial reduction of 60% after 3 h treatment, and 90% after 6 h incubation). Furthermore, the nanocomposite displayed moderate cytotoxicity or hemolysis effect, with cellular viabilities over 85% at concentrations up to 16 times the MIC value. Studies on antibacterial mechanism revealed that the synergy between PMB molecules and GO nanosheets greatly facilitated the vertical insertion of the nanocomposite into the lipid membrane, leading to membrane disturbance and permeabilization. Our results demonstrate a physical mechanism for improving the antibacterial performance of PMB and developing advanced antibacterial agents for better clinic uses.
Liquid Phase Infiltration of Block Copolymers
Authors: Murataj Irdi , Cara Eleonora , Baglieri Nicoletta , Pirri Candido Fabrizio , De Leo Natascia , Ferrarese Lupi Federico
Published: October 2022

Novel materials with defined composition and structures at the nanoscale are increasingly desired in several research fields spanning a wide range of applications. The development of new approaches of synthesis that provide such control is therefore required in order to relate the material properties to its functionalities. Self-assembling materials such as block copolymers (BCPs), in combination with liquid phase infiltration (LPI) processes, represent an ideal strategy for the synthesis of inorganic materials into even more complex and functional features. This review provides an overview of the mechanism involved in the LPI, outlining the role of the different polymer infiltration parameters on the resulting material properties. We report newly developed methodologies that extend the LPI to the realisation of multicomponent and 3D inorganic nanostructures. Finally, the recently reported implementation of LPI into different applications such as photonics, plasmonics and electronics are highlighted.
Additive Manufacturing of Drug-Eluting Multilayer Biodegradable Films
Authors: Proshin Pavel I. , Abdurashitov Arkady S. , Sindeeva Olga A. , Ivanova Anastasia A. , Sukhorukov Gleb B.
Published: October 2022

Drug-eluting films made of bioresorbable polymers are a widely used tool of modern personalized medicine. However, most currently existing methods of producing coatings do not go beyond the laboratory, as they have low encapsulation efficiency and/or difficulties in scaling up. The PLACE (Printed Layered Adjustable Cargo Encapsulation) technology proposed in this article uses an additive approach for film manufacturing. PLACE technology is accessible, scalable, and reproducible in any laboratory. As a demonstration of the technology capabilities, we fabricated layered drug-eluting polyglycolic acid films containing different concentrations of Cefazolin antibiotic. The influence of the amount of loaded drug component on the film production process and the release kinetics was studied. The specific loading of drugs was significantly increased to 200–400 µg/cm2 while maintaining the uniform release of Cefazolin antibiotic in a dosage sufficient for local antimicrobial therapy for 14 days. The fact that the further increase in the drug amount results in the crystallization of a substance, which can lead to specific defects in the cover film formation and accelerated one-week cargo release, was also shown, and options for further technology development were proposed.
Microwave-Assisted Lignin Extraction—Utilizing Deep Eutectic Solvents to Their Full Potential
Authors: Meindl Alina , Petutschnigg Alexander , Schnabel Thomas
Published: October 2022

The current research intended to investigate the suitability of different choline-chloride-based deep eutectic solvents for their role in microwave lignin extraction. Lignin, a widely spread biopolymer in plants and woody structures, is a valuable replacement for fossil-fuel-based materials. While some promising applications have been trialled already, the extraction of this material from its matrix still causes problems. Here, we highlight an efficient and fast method to extract lignin from untreated larch bark with deep eutectic solvents in a standard domestic microwave. We developed a straightforward, green methodology, which can be used on various reaction scales, with materials available to many researchers. Lignin was extracted within only 30 min of microwave irradiation in yields of up to 96%. Compared to traditional deep eutectic extraction by conventional heating, the reaction time was cut by 87% and the energy costs were reduced by 93.5%. The hydrogen bond donors were exchanged and different types, namely acid-based, hydroxyl-based and amide-based donor systems, were evaluated for their suitability concerning microwave lignin extraction. This study presents a novel approach towards energy-efficient and green lignin valorisation, without the inherent need for costly equipment.
Progress in the Preparation, Properties, and Applications of PLA and Its Composite Microporous Materials by Supercritical CO2: A Review from 2020 to 2022
Authors: Peng Kangming , Mubarak Suhail , Diao Xuefeng , Cai Zewei , Zhang Chen , Wang Jianlei , Wu Lixin
Published: October 2022

The development of degradable plastic foams is in line with the current development concept of being pollution free and sustainable. Poly(lactic acid) (PLA) microporous foam with biodegradability, good heat resistance, biocompatibility, and mechanical properties can be successfully applied in cushioning packaging, heat insulation, noise reduction, filtration and adsorption, tissue engineering, and other fields. This paper summarizes and critically evaluates the latest research on preparing PLA microporous materials by supercritical carbon dioxide (scCO2) physical foaming since 2020. This paper first introduces the scCO2 foaming technologies for PLA and its composite foams, discusses the CO2-assisted foaming processes, and analyzes the effects of process parameters on PLA foaming. After that, the paper reviews the effects of modification methods such as chemical modification, filler filling, and mixing on the rheological and crystallization behaviors of PLA and provides an in-depth analysis of the mechanism of PLA foaming behavior to provide theoretical guidance for future research on PLA foaming. Lastly, the development and applications of PLA microporous materials based on scCO2 foaming technologies are prospected.
High Performance Composites Based on Highly Filled Glass Fiber-Reinforced Polybenzoxazine for Post Application
Authors: Mora Phattarin , Nunwong Chananya , Sriromreun Parkpoom , Kaewsriprom Preecha , Srisorrachatr Ukrit , Rimdusit Sarawut , Jubsilp Chanchira
Published: October 2022

Glass fiber post based on the new polymeric material, polybenzoxazine, is prepared and the effects of glass fiber contents on mechanical and thermal properties are evaluated. The mechanical response to externally applied loads of tooth restored with glass fiber-reinforced polybenzoxazine composite posts is also simulated by finite element analysis of a tridimensional model and compared with the response to that of a natural tooth. The reinforcing of glass fiber can help improve the mechanical and thermal properties of the polybenzoxazine influenced by the interfacial adhesion between the glass fiber and polybenzoxazine matrix, except for the relatively high mechanical property of the glass fiber. The mechanical data, i.e., elastic modulus under flexure load or flexural modulus by three-point bending test of the glass fiber-reinforced polybenzoxazine composites are agreed with the elastic modulus of dentin and then used in the finite element model. The restoration using the glass fiber-reinforced polybenzoxazine composite post provided the maximum von Mises equivalent stress at the cervical third area of the endodontically treated tooth model as similarly observed in the natural tooth. In addition, the maximum von Mises equivalent stress of the tooth restored with the glass fiber-reinforced polybenzoxazine composite post is also quietly like that of the natural tooth. The finding of this work provided the essential properties of the glass fiber-reinforced polybenzoxazine composite for dental restorations and appliances.
Polyelectrolyte Chondroitin Sulfate Microgels as a Carrier Material for Rosmarinic Acid and Their Antioxidant Ability
Authors: Sahiner Mehtap , Suner Selin S. , Yilmaz Aynur S. , Sahiner Nurettin
Published: October 2022

Polyelectrolyte microgels derived from natural sources such as chondroitin sulfate (CS) possess considerable interest as therapeutic carriers because of their ionic nature and controllable degradation capability in line with the extent of the used crosslinker for long-term drug delivery applications. In this study, chemically crosslinked CS microgels were synthesized in a single step and treated with an ammonia solution to attain polyelectrolyte CS−[NH4]+ microgels via a cation exchange reaction. The spherical and non-porous CS microgels were injectable and in the size range of a few hundred nanometers to tens of micrometers. The average size distribution of the CS microgels and their polyelectrolyte forms were not significantly affected by medium pH. It was determined that the −34 ± 4 mV zeta potential of the CS microgels was changed to −23 ± 3 mV for CS− [NH4]+ microgels with pH 7 medium. No important toxicity was determined on L929 fibroblast cells, with 76 ± 1% viability in the presence of 1000 μg/mL concentration of CS−[NH4]+ microgels. Furthermore, these microgels were used as a drug carrier material for rosmarinic acid (RA) active agent. The RA-loading capacity was about 2.5-fold increased for CS−[R]+ microgels with 32.4 ± 5.1 μg/mg RA loading, and 23% of the loaded RA was sustainably release for a long-term period within 150 h in comparison to CS microgels. Moreover, RA-loaded CS−[R]+ microgels exhibited great antioxidant activity, with 0.45 ± 0.02 μmol/g Trolox equivalent antioxidant capacity in comparison to no antioxidant properties for bare CS particles.
Evaluation of the Orthotropic Behavior in an Auxetic Structure Based on a Novel Design Parameter of a Square Cell with Re-Entrant Struts
Authors: Valle Rodrigo , Pincheira Gonzalo , Tuninetti Víctor , Garrido Cesar , Treviño Cecilia , Morales Jorge
Published: October 2022

In this research, a three-dimensional auxetic configuration based on a known re-entrant cell is proposed. The 3D auxetic cell is configured from a new design parameter that produces an internal rotation angle to its re-entrant elements to study elastic properties in its three orthogonal directions. Through a topological analysis using Timoshenko beam theory, the bending of its re-entrant struts is modeled as a function of the new design parameter to manipulate Poisson’s ratio and Young’s modulus. Experimental samples were fabricated using a fused filament fabrication system using ABS and subsequently tested under quasi-static compression and bending tests. Additionally, an orthotropy factor is applied that allows for measuring the deviation between the mechanical properties of each structure. The experimental results validate the theoretical design and show that this new unit cell can transmit an orthotropic mechanical behavior to the macrostructure. In addition, the proposed structure can provide a different bending stiffness behavior in up to three working directions, which allows the application under different conditions of external forces, such as a prosthetic ankle.