bluuebunny/crossref_metadata_2025 huggingface dataset:
A Comparative Thermal and Economic Investigation of Similar Shell & Tube and Plate Heat Exchangers with Low Concentration Ag-H2O Nanofluid
Authors: Pourhoseini Seyed Hadi , Baghban Mojtaba , Ghodrat Maryam
Published: February 2023

Plate Heat Exchanger (PHE) and Shell and Tube Heat Exchanger (STHE) with identical heat transfer areas and material characteristics are proposed and a comparative thermal and economic comparative analysis is carried out on both exchangers. Ag-water nanofluid is used at low concentrations (0, 2.5, 5, 10 mg/L), flow rates (2, 5, and 8 L/min), and inlet temperatures (36, 46, and 56 °C) as hot flow and the heat transfer coefficient (U), electrical power consumption of the pump, and costs per unit of average U value are considered as the calculated parameters for each heat exchanger in co-current and counter-current flows. The results revealed that PHE generates a higher U value compared to the STHE under different Ag-water nanofluid concentrations. This is due to the existence of grooves on the plates of PHE which generates turbulent flow. The impact of nanofluid concentration on U is negligible for lower concentrations in both PHE and STHE. It is also found that the nanofluid flow rate has the highest impact on the U value, just like conventional fluid. Besides, even though counter-current flow increases the U values for both PHE and STHE, the flow pattern has a higher impact on the U value of PHE than that of STHE. For both PHE and STHE, increasing the nanofluid flow rate enhances the amount of U. However, the effect of flow rate on the U value of PHE is greater than that of the STHE. It is also shown that throughout the entire experimental temperature domain, PHE has had higher performance than STHE, and as the fluid temperature increased from 36 to 56 °C, there was a slight increase in the overall heat transfer of both PHE and STHE. Furthermore, for the same flow rate, both PHE and STHE had almost the same pump power consumption, and increasing the nanofluid flow rate from 2 L/min to 8 L/min promoted the electrical power consumption of the pump. Finally, we found that the costs per unit of heat transfer coefficient for PHE are significantly lower than STHE. The presented results also indicated that using a vortex generator at the inlet of STHE tubes, to form turbulent flow, increases the U values of STHE for both co-current and counter-current flows but these U values are lower than the corresponding U values of PHE. Small plates gap in PHE structure cause higher fluid flow velocities and create a chain-like structure of nanoparticles (NPs) between PHE’s plates (especially at higher nanofluids concentrations).
Grouping Control Strategy for Battery Energy Storage Power Stations Considering the Wind and Solar Power Generation Trend
Authors: Guo Wei , Fan Wenyi , Zhao Yang , An Jiakun , He Chunguang , Guo Xiaomei , Qian Yanan , Ma Libo , Zhao Hongshan
Published: February 2023

For the optimal power distribution problem of battery energy storage power stations containing multiple energy storage units, a grouping control strategy considering the wind and solar power generation trend is proposed. Firstly, a state of charge (SOC) consistency algorithm based on multi-agent is proposed. The adaptive power distribution among the units started can be realized using this algorithm. Then, considering the trend of wind and solar power generation, a reasonable grouping control strategy is formulated. The grouping situation of the units is determined by using the probability distribution characteristics of energy storage charging and discharging, which reduces the number of charging and discharging conversions and extends the power station life. Finally, the actual data of a wind–solar energy storage microgrid is used to verify the method. The simulation results demonstrate that the proposed method has certain advantages in terms of control effect, SOC consistency, and extending the power station life.
A Hybrid Model of VMD-EMD-FFT, Similar Days Selection Method, Stepwise Regression, and Artificial Neural Network for Daily Electricity Peak Load Forecasting
Authors: Aswanuwath Lalitpat , Pannakkong Warut , Buddhakulsomsiri Jirachai , Karnjana Jessada , Huynh Van-Nam
Published: February 2023

Daily electricity peak load forecasting is important for electricity generation capacity planning. Accurate forecasting leads to saving on excessive electricity generating capacity, while maintaining the stability of the power system. The main challenging tasks in this research field include improving forecasting accuracy and reducing computational time. This paper proposes a hybrid model involving variational mode decomposition (VMD), empirical mode decomposition (EMD), fast Fourier transform (FFT), stepwise regression, similar days selection (SD) method, and artificial neural network (ANN) for daily electricity peak load forecasting. Stepwise regression and similar days selection method are used for input variable selection. VMD and FFT are applied for data decomposition and seasonality capturing, while EMD is employed for determining an appropriate decomposition level for VMD. The hybrid model is constructed to effectively forecast special holidays, which have different patterns from other normal weekdays and weekends. The performance of the hybrid model is tested with real electricity peak load data provided by the Electricity Generating Authority of Thailand, the leading power utility state enterprise under the Ministry of Energy. Experimental results show that the hybrid model gives the best performance while saving computation time by solving the problems in input variable selection, data decomposition, and imbalance data of normal and special days in the training process.
Implementing Partnerships in Energy Supply Chain Cybersecurity Resilience
Authors: Wallis Tania , Dorey Paul
Published: February 2023

This study describes the implementation of an energy sector community to examine the practice of cybersecurity for operational technology environments and their supply chains. Evaluating cybersecurity from the perspectives of different actors participating in the energy sector, the progress and challenges of operators and suppliers in delivering cybersecurity for the sector are explored. While regulatory frameworks incentivize individual organizations to improve their cybersecurity, operational services contain contributions from many organizations, and this supply chain of activity needs to be influenced and managed to achieve desired security and resilience outcomes. Through collaborations and systems engineering approaches, a reference model is created to facilitate improvements in managing the cybersecurity of supply chains for different actors, including service operators, maintainers, manufacturers, and systems integrators. This study provides an illustration of implementing a common vision of cybersecurity improvement across a community of actors. It utilizes a collaborative framework that has facilitated the co-production of cybersecurity guidance for energy sector participants.
Nanostructured Electrocatalysts for Advanced Applications in Fuel Cells
Authors: Omeiza Lukman Ahmed , Abdalla Abdalla M. , Wei Bo , Dhanasekaran Anitha , Subramanian Yathavan , Afroze Shammya , Reza Md Sumon , Bakar Saifullah Abu , Azad Abul Kalam
Published: February 2023

Nanostructured materials have gained much attention in recent engineering and material- science research due to their unique structural makeup, which stands them out from their bulk counterparts. Their novel properties of tiny-size structural elements (molecules or crystallites, clusters) of nanoscale dimensions (1 to 100 nm) make them a perfect material for energy applications. The recent keen interest in nanostructured materials research by academia and industrial experts arises from the unique variable characteristics of increased electrical and thermal conductivity. This occurs as nanostructured materials undergo a transient process from infinite-extended solid to a particle of ascertainable numbers of atoms. The commercial and energy sectors are very interested in developing and expanding simple synthetic pathways for nanostructured-electrocatalysts materials to aid in optimizing the number of active regions. Over the decades, various techniques have been put forward to design and synthesize nanostructured-electrocatalysts materials for electrochemical generation of energy and storage applications. As a result, the design of fuel cells, supercapacitors, and energy-storage devices has advanced significantly. This review provides a comprehensive outlook of various synthesis techniques and highlight the challenges of nanostructured- electrocatalysts materials application in fuel cells. Several synthesis methods are discussed and summarized for enhanced nanomaterial preparation and high product attainment with the sol-gel synthesis method being emphasized. The design methodology for an effective nanostructured electrocatalysts with high efficiency for fuel cells was also discussed.
Effects of Solids Accumulation on Greenhouse Gas Emissions, Substrate, Plant Growth and Performance of a Mediterranean Horizontal Flow Treatment Wetland
Authors: Sacco Alessandro , Sciuto Liviana , Licciardello Feliciana , Cirelli Giuseppe L. , Milani Mirco , Barbera Antonio C.
Published: February 2023

In treatment wetlands (TWs), solids accumulation can result in hydraulic malfunction, reducing the operation life, and it could enhance biological activity by favoring biofilm development. It is still unknown whether the solids accumulation can affect greenhouse gas (GHG) emissions. This study aims to evaluate the solid concentration along a horizontal flow (HF) TW, and its role in GHG emissions, hydraulics, treatment performance, and vegetation development (Phragmites australis (Cav.) Trin. ex Steud.). The study was carried out in an eight-year-old full-scale HF-TW located in the Mediterranean region (Sicily, Italy). To collect data inside the HF unit, nine observation points (besides the inlet and the outlet) along three 8.5-m-long transects (T1, T2, and T3) were identified. The first transect (close to the inlet zone) showed a hydraulic conductivity (Ks) reduction approximately one order of magnitude higher than the other two. Results highlighted that GHG emissions increased during the summer, when the temperature and solar radiation were higher than in the rest of the year, matching the macrophyte growth rate. Theoretical methane (CH4) emissions followed the trend of volatile solids (VS), which was around 3.5 and 4 times in T1 to T2 and T3. Pore clogging affected carbon dioxide (CO2) emissions, which decreased from T1 to T3, with maximum monthly values in T1 (21.4 g CO2·m−2·d−1) being approximately double with respect to T2 (12.6 g CO2·m−2·d−1) and T3 (10.7 g CO2·m−2·d−1) observed in July. The same trend for chemical oxygen demand (COD) removal efficiency, decreasing from T1 to T3, was observed. Notwithstanding this behavior, the final effluent quality was very satisfactory, with an average value of COD removal efficiency above 90%.
High Abundance of Candidatus Arthromitus in Intestinal Microbiota of Seriolella violacea (Palm Ruff) under Reared Conditions
Authors: Romero Jaime , Catalán Natalia , Ramírez Carolina , Miranda Claudio , Oliva Marcia , Flores Héctor , Romero María , Rojas Rodrigo
Published: February 2023

Intestinal microbiota has been involved in several processes that benefit the host, such as digestion, nutrient metabolism, resistance to pathogens colonization and immune function. In this study, we investigated the diversity, composition and functional prediction of microbiota of reared Seriolella violacea (palm ruff) in the same cohort sampled at different times (7-, 8- and 9-month-old). Microbial community structure analyses, using 16S rRNA amplicon sequencing, revealed that the intestinal microbiota was dominated by the phyla Firmicutes, Proteobacteria, Fusobacteria and Tenericutes. At the genus level, Candidatus Arthromitus was the most abundant in all sampled timepoints, representing in average 78% of the bacterial community (ranging from 18 to 98%), corresponding to segmented filamentous bacteria, which are interesting because they have been associated with the maturation of immune responses in the gut and protecting the host from bacterial infections. The comparisons of the intestinal microbiota among the three groups showed differences in abundance of bacterial taxa and also in alpha diversity indexes (Shannon and Simpson), as well as beta diversity metrics (weighted and unweighted UniFrac). Potential functions of the intestinal microbiota of palm ruff were retrieved using Philipin and Tax4fun and these analyses revealed high levels of genes for sugar metabolism. To our knowledge, this study represents the first description of the intestinal microbiota of S. violacea.
Apple Pomace as an Ingredient Enriching Wheat Pasta with Health-Promoting Compounds
Authors: Gumul Dorota , Kruczek Marek , Ivanišová Eva , Słupski Jacek , Kowalski Stanisław
Published: February 2023

The global overproduction of apples is associated with large amounts of post-production waste, for which new forms of utilization should be sought. Therefore, we aimed to enrich wheat pasta with apple pomace in various percentages (10, 20, 30 and 50%). The content of total polyphenols, individual polyphenols (using UPLC-PDA-MS/MS methods) and dietary fibre, chemical composition and physical properties of the resulting pasta were determined. The addition of apple pomace to pasta resulted in increased levels of pro-health compounds: total polyphenols, phenolic acids, quercetin derivatives, flavon-3-ols and dihydrochalcones as well as dietary fibre. Decreases in hardness and maximum cutting energy were also observed in pasta supplemented with apple pomace compared to control pasta. Water absorption capacity was not influenced by the addition of apple pomace, with the exception of pasta made with 50% apple pomace.
A Comparative Study on the Meat Quality, Taste and Aroma Related Compounds between Korean Hanwoo and Chikso Cattle
Authors: Hoa Van-Ba , Song Dong-Heon , Seol Kuk-Hwan , Kang Sun-Moon , Kim Hyun-Wook , Bae In-Seon , Kim Eun-Sung , Park Yeon-Soo , Cho Soo-Hyun
Published: February 2023

The aim of this study was to compare the meat quality and taste-and-aroma-related components of beef between breeds. For this purpose, Hanwoo and Chikso steers (n = 7 per breed) raised under identical conditions until 30 months old were used. After 24 h of slaughter, longissimus lumborum (LL) and semimembranosus (SM) muscles were collected and analyzed for technological quality, free amino acids, metabolites, and volatile compounds. The Chikso meat showed lower values for shear force and color traits (lightness, redness, and yellowness) compared to Hanwoo (p < 0.05). The Chikso presented a higher amount of sweetness-related free amino acids (alanine, proline, and threonine) in the LL muscle, while Hanwoo had a higher amount of methionine and glutamine associated with umami taste (p < 0.05). A total of 36 metabolites were identified and quantified in the meat samples; out of them, 7 compounds were affected by breed (p < 0.05). Regarding aroma compounds, a significantly higher amount of fat-derived aldehydes associated with fatty and sweet notes was found in Hanwoo, whereas a higher amount of pyrazines associated with roasty notes was found in Chikso (p < 0.05). Thus, under identical feeding conditions, breed showed a significant effect on the quality and taste-and-aroma-related components that may influence the eating quality of beef between the two breeds studied.
Role of PI3K-AKT Pathway in Ultraviolet Ray and Hydrogen Peroxide-Induced Oxidative Damage and Its Repair by Grain Ferments
Authors: Cheng Wenjing , Shi Xiuqin , Zhang Jiachan , Li Luyao , Di Feiqian , Li Meng , Wang Changtao , An Quan , Zhao Dan
Published: February 2023

UV and external environmental stimuli can cause oxidative damage to skin cells. However, the molecular mechanisms involved in cell damage have not been systematically and clearly elucidated. In our study, an RNA-seq technique was used to determine the differentially expressed genes (DEGs) of the UVA/H2O2-induced model. Gene Oncology (GO) clustering and the Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway analysis were performed to determine the core DEGs and key signaling pathway. The PI3K-AKT signaling pathway was selected as playing a part in the oxidative process and was verified by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). We selected three kinds of Schizophyllum commune fermented actives to evaluate whether the PI3K-AKT signaling pathway also plays a role in the resistance of active substances to oxidative damage. Results indicated that DEGs were mainly enriched in five categories: external stimulus response, oxidative stress, immunity, inflammation, and skin barrier regulation. S. commune-grain ferments can effectively reduce cellular oxidative damage through the PI3K-AKT pathway at both the cellular and molecular levels. Some typical mRNAs (COL1A1, COL1A2, COL4A5, FN1, IGF2, NR4A1, and PIK3R1) were detected, and the results obtained were consistent with those of RNA-seq. These results may give us a common set of standards or criteria for the screen of anti-oxidative actives in the future.
Amino-Functionalized Cellulose Nanofiber/Lignosulfonate New Aerogel Adsorbent for the Removal of Dyes and Heavy Metals from Wastewater
Authors: Elsayed Islam , Schueneman Gregory T. , El-Giar Emad M. , Hassan El Barbary
Published: February 2023

Due to the increasingly widespread water pollutants and the high cost of treatment methods, there is a demand for new, inexpensive, renewable, and biodegradable adsorbent materials for the purification of wastewater contaminants. In this study, a new biocomposite aerogel (Amf-CNF/LS) was prepared using a chemically cross-linking method between the amino-functionalized cellulose nanofibers (Amf-CNF) and lignosulfonates (LS). The physical and chemical properties of the prepared aerogel were investigated using several techniques including elemental analysis, scanning electron microscopy (SEM-EDS), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), and N2 adsorption-desorption analysis. The Amf-CNF/LS aerogel was then applied for the removal of methylene blue (MB), rhodamine B dye (RhB), and the heavy metal cadmium ion (Cd2+) from synthetic wastewater solutions. The adsorption parameters controlling the adsorption process including the pH, contact time, adsorbent dosage, and adsorbate concen-tration were optimized. High adsorption kinetics and isotherms were observed, with the adsorption isotherms of the Amf-CNF/LS aerogel fitting the Langmuir model with maximum adsorption capacities of 170.94, 147.28, and 129.87 mg/g for MB, RhB, and Cd2+, respectively. These results show that Amf-CNF/LS aerogel is a promising green and inexpensive adsorbent for MB, RhB, and Cd2+ removal from wastewater.
Functional Exercise Versus Specific Pelvic Floor Exercise: Observational Pilot Study in Female University Students
Authors: Díaz-Mohedo Esther , Odriozola Aguirre Itxaso , Molina García Elena , Infantes-Rosales Miguel Angel , Hita-Contreras Fidel
Published: February 2023

Objectives: To evaluate the electromyographic (EMG) activity of the pelvic floor musculature (PFM) that takes place when performing the functional movement screen (FMS) exercise, comparing it with the activation in the maximum voluntary contraction of PFM in the supine position (MVC-SP) and standing (MVC-ST). Material and Methods: A descriptive, observational study conducted in two phases. In the first study phase, the baseline EMG activity of PFM was measured in the supine position and standing during MVC-SP and MVC-ST and during the execution of the seven exercises that make up the FMS. In the second phase of the study, the baseline EMG activity of PFM was measured in the supine position and standing during MVC-SP and MVC-ST and during the FMS exercise that produced the most EMG in the pilot phase: trunk stability push-up (PU). ANOVA, Friedman’s and Pearson’s tests were used. Results: All FMS exercises performed in the pilot phase showed a value below 100% maximum voluntary contraction (MVC) except PU, which presented an average value of 101.3 μv (SD = 54.5): 112% MVC (SD = 37.6). In the second phase of the study, it was observed that there were no significant differences (p = 0.087) between the three exercises performed: MVC-SP, MVC-ST and PU (39.2 μv (SD = 10.4), 37.5 μv (SD = 10.4) and 40.7 μv (SD = 10.2), respectively). Conclusions: There is no evidence of the existence of significant differences in EMG activation in PFM among the three exercises analysed: MVC-SP, MVC-ST and PU. The results show better EMG values in the functional exercise of PU.
Study of Burden in Polycystic Ovary Syndrome at Global, Regional, and National Levels from 1990 to 2019
Authors: Gao Yong , Liu Haobiao , Qiao Lichun , Liang Jiawei , Yao Haoyan , Lin Xue , Gao Yane
Published: February 2023

Increasing attention has recently been paid to the harm of polycystic ovary syndrome (PCOS) to women. However, due to the inconsistency of global clinical diagnostic standards and the differing allocation of medical resources among different regions, there is a lack of comprehensive estimation of the global incidence and disability-adjusted life years (DALYs) of PCOS. Thus, it is difficult to assess the disease burden. We extracted PCOS disease data from 1990 to 2019 from the Global Burden of Disease Study (GBD) 2019 and estimated the incidence, DALYs, and the corresponding age-standardized rates (ASRs) of PCOS, as well as the socio-demographic index (SDI) quintiles, to describe epidemiological trends at the global level, encompassing 21 regions and 204 countries and territories. Globally, the incidence and DALYs of PCOS have increased. Its ASR also shows an increasing trend. Among them, the high SDI quintile seems relatively stable, whereas other SDI quintiles are constantly rising over time. Our research has provided clues regarding the disease pattern and epidemic trend of PCOS and analyzed the possible causes of disease burden in some specific countries and territories, which may have some value in health resource allocation and health policy formulation and prevention strategies.
NFT Digital Twins: A Digitalization Strategy to Preserve and Sustain Miao Silver Craftsmanship in the Metaverse Era
Authors: Wang Mingke , Lau Newman
Published: February 2023

Miao silver, generally referred to as the unique silver ornaments manufactured by the Miao ethnicity with over 400 years of history, was listed as a Chinese national intangible cultural heritage in 2006. Through primary research methods, including local investigation, interviews, and workshops at Maliao Village in Guizhou, this study found that sizeable industrial production shrank the market needs for traditional Miao silver handicrafts. A growing number of young Miao people pursue higher incomes and leave their hometowns to make a living in cities instead of engaging in Miao silver handcrafting in villages. Based on the difficulties encountered in the inheritance of Miao silver craftsmanship, this project creatively proposed a digital strategy based on the NFT digital twin application to preserve and maintain Miao silver technology in the context of the metaverse era. By conducting design experiments combining digital technology and the traditional Miao silver manufacturing techniques, a collection of NFT digital twins, digital duplications of Miao silver handicrafts that Miao silversmith jointly worked with 3D technologist, was created as a vital research outcome. The results show that this attempt is beneficial to help expand the use scenarios, extend the lifespan, and increase the commercial value of Miao silver. To sum up, taking Miao silverware handicrafts as a research case, this paper dialectically discusses the relationship between industrial manufacturing and traditional handicraft industries, making contributions to the inheritance and development of Miao silver and also putting forward innovative solutions to promote the prosperity of intangible cultural heritage worldwide.
Enhancing Rosemary (Rosmarinus officinalis, L.) Growth and Volatile Oil Constituents Grown under Soil Salinity Stress by Some Amino Acids
Authors: Al-Fraihat Ahmad H. , Al-Dalain Sati Y. , Zatimeh Ahmad A. , Haddad Moawiya A.
Published: February 2023

The current study was carried out during the two consecutive winter seasons of 2019/2020 and 2020/2021 at the Experimental Farm of Ash-Shoubak University College, Jordan, as a pot experiment. This experiment was planned to evaluate the impact of various soil salinity levels (1.17, 3.34, 6.51, and 9.68 ds/m) and amino acid types (control, L-tryptophan acid at 100 ppm, glutamine acid at 200 ppm, and L-tryptophan acid + glutamine acid) as well as their combinations on growth, salt resistance index, and some of the chemical constituents of rosemary plants. The obtained results indicated that plant height and the total herb dry weight of rosemary, the salt resistance index (SRI), and the total chlorophyll in leaves were discernably reduced with increasing soil salinity levels compared with the control. However, salinity enhanced leaf proline content. Each amino acid or its mixture improved plant growth, chlorophyll content, and SRI parameters. The SRI percentage of R. officinalis enhanced to more than 100% under a soil salinity level of 1.17 ds/m, combined with amino acids at any type, when compared with the other combination treatments. Furthermore, GC/MS showed that the identified compounds ranged from 98.39% to 99.18% and the unidentified compounds from 0.82% to 1.61% from the volatile oil of rosemary plants. The major constituents of volatile oil samples were camphor (34.95% to 40.21%), D-verbenone (13.74% to 15.23%), and α-pinene (13.21% to 16.73%).
Paclobutrazol Promotes Root Development of Difficult-to-Root Plants by Coordinating Auxin and Abscisic Acid Signaling Pathways in Phoebe bournei
Authors: Li Jing , Xu Peiyue , Zhang Baohong , Song Yanyan , Wen Shizhi , Bai Yujie , Ji Li , Lai Yong , He Gongxiu , Zhang Dangquan
Published: February 2023

Phoebe bournei is a rare and endangered plant endemic to China with higher-value uses in essential oil and structural wood production. Its seedlings are prone to death because of its undeveloped system. Paclobutrazol (PBZ) can improve root growth and development in certain plants, but its concentration effect and molecular mechanism remain unclear. Here, we studied the physiological and molecular mechanisms by which PBZ regulates root growth under different treatments. We found that, with moderate concentration treatment (MT), PBZ significantly increased the total root length (69.90%), root surface area (56.35%), and lateral root number (47.17%). IAA content was the highest at MT and was 3.83, 1.86, and 2.47 times greater than the control, low, and high-concentration treatments. In comparison, ABA content was the lowest and reduced by 63.89%, 30.84%, and 44.79%, respectively. The number of upregulated differentially expressed genes (DEGs) induced at MT was more than that of down-regulated DEGs, which enriched 8022 DEGs in response to PBZ treatments. WGCNA showed that PBZ-responsive genes were significantly correlated with plant hormone content and involved in plant hormone signal transduction and MAPK signal pathway-plant pathways, which controls root growth. The hub genes are observably associated with auxin, abscisic acid syntheses, and signaling pathways, such as PINs, ABCBs, TARs, ARFs, LBDs, and PYLs. We constructed a model which showed PBZ treatments mediated the antagonism interaction of IAA and ABA to regulate the root growth in P. bournei. Our result provides new insights and molecular strategies for solving rare plants’ root growth problems.
Neurodevelopmental Toxicity of Emamectin Benzoate to the Early Life Stage of Zebrafish Larvae (Danio rerio)
Authors: Gu Jie , Guo Liguo , Zhu Yuanhui , Qian Lingling , Shi Lili , Zhang Huanchao , Ji Guixiang
Published: February 2023

Emamectin benzoate (EMB) is a widely used pesticide and feed additive in agriculture and aquaculture. It easily enters the aquatic environment through various pathways, thus causing adverse effects on aquatic organisms. However, there are no systematic studies regarding the effects of EMB on the developmental neurotoxicity of aquatic organisms. Therefore, the aim of this study was to evaluate the neurotoxic effects and mechanisms of EMB at different concentrations (0.1, 0.25, 0.5, 1, 2, 4 and 8 μg/mL) using zebrafish as a model. The results showed that EMB significantly inhibited the hatching rate, spontaneous movement, body length, and swim bladder development of zebrafish embryos, as well as significantly increased the malformation rate of zebrafish larvae. In addition, EMB adversely affected the axon length of motor neurons in Tg (hb9: eGFP) zebrafish and central nervous system (CNS) neurons in Tg (HuC: eGFP) zebrafish and significantly inhibited the locomotor behavior of zebrafish larvae. Meanwhile, EMB induced oxidative damage and was accompanied by increasing reactive oxygen species in the brains of zebrafish larvae. In addition, gene expression involvement in oxidative stress-related (cat, sod and Cu/Zn-sod), GABA neural pathway-related (gat1, gabra1, gad1b, abat and glsa), neurodevelopmental-related (syn2a, gfap, elavl3, shha, gap43 and Nrd) and swim bladder development-related (foxa3, pbxla, mnx1, has2 and elovlla) genes was significantly affected by EMB exposure. In conclusion, our study shows that exposure to EMB during the early life stages of zebrafish significantly increases oxidative damage and inhibits early central neuronal development, motor neuron axon growth and swim bladder development, ultimately leading to neurobehavioral changes in juvenile zebrafish.
Use of 3D Spheroid Models for the Assessment of RT Response in Head and Neck Cancer
Authors: Wegge Marilyn , Dok Rüveyda , Dubois Ludwig J. , Nuyts Sandra
Published: February 2023

Radiotherapy (RT) is a key player in the treatment of head and neck cancer (HNC). The RT response, however, is variable and influenced by multiple tumoral and tumor microenvironmental factors, such as human papillomavirus (HPV) infections and hypoxia. To investigate the biological mechanisms behind these variable responses, preclinical models are crucial. Up till now, 2D clonogenic and in vivo assays have remained the gold standard, although the popularity of 3D models is rising. In this study, we investigate the use of 3D spheroid models as a preclinical tool for radiobiological research by comparing the RT response of two HPV-positive and two HPV-negative HNC spheroid models to the RT response of their corresponding 2D and in vivo models. We demonstrate that HPV-positive spheroids keep their higher intrinsic radiosensitivity when compared to HPV-negative spheroids. A good correlation is found in the RT response between HPV-positive SCC154 and HPV-negative CAL27 spheroids and their respective xenografts. In addition, 3D spheroids are able to capture the heterogeneity of RT responses within HPV-positive and HPV-negative models. Moreover, we demonstrate the potential use of 3D spheroids in the study of the mechanisms underlying these RT responses in a spatial manner by whole-mount Ki-67 and pimonidazole staining. Overall, our results show that 3D spheroids are a promising model to assess the RT response in HNC.
Dipolarophile-Controlled Regioselective 1,3-Dipolar Cycloaddition: A Switchable Divergent Access to Functionalized N-Fused Pyrrolidinyl Spirooxindoles
Authors: Wang Yongchao , Yan Lijun , Yan Yuxin , Li Sujin , Lu Hongying , Liu Jia , Dong Jianwei
Published: February 2023

N-fused pyrrolidinyl spirooxindole belongs to a class of privileged heterocyclic scaffolds and is prevalent in natural alkaloids and synthetic pharmaceutical molecules. To realize the switchable synthesis of divergent N-fused pyrrolidinyl spirooxindoles for further biological activity evaluation via a substrate-controlled strategy, a chemically sustainable, catalysis-free, and dipolarophile-controlled three-component 1,3-dipolar cycloaddition of isatin-derived azomethine ylides with diverse dipolarophiles is described in this work. A total of 40 functionalized N-fused pyrrolidinyl spirooxindoles were synthesized in 76–95% yields with excellent diastereoselectivities (up to >99:1 dr). The scaffolds of these products can be well-controlled by employing different 1,4-enedione derivatives as dipolarophiles in EtOH at room temperature. This study provides an efficient strategy to afford a spectrum of natural-like and potentially bioactive N-fused pyrrolidinyl spirooxindoles.
Mitochondrial DNA Deficiency and Supplementation in Sus scrofa Oocytes Influence Transcriptome Profiles in Oocytes and Blastocysts
Authors: Okada Takashi , McIlfatrick Stephen , St. John Justin C.
Published: February 2023

Mitochondrial DNA (mtDNA) deficiency correlates with poor oocyte quality and fertilisation failure. However, the supplementation of mtDNA deficient oocytes with extra copies of mtDNA improves fertilisation rates and embryo development. The molecular mechanisms associated with oocyte developmental incompetence, and the effects of mtDNA supplementation on embryo development are largely unknown. We investigated the association between the developmental competence of Sus scrofa oocytes, assessed with Brilliant Cresyl Blue, and transcriptome profiles. We also analysed the effects of mtDNA supplementation on the developmental transition from the oocyte to the blastocyst by longitudinal transcriptome analysis. mtDNA deficient oocytes revealed downregulation of genes associated with RNA metabolism and oxidative phosphorylation, including 56 small nucleolar RNA genes and 13 mtDNA protein coding genes. We also identified the downregulation of a large subset of genes for meiotic and mitotic cell cycle process, suggesting that developmental competence affects the completion of meiosis II and first embryonic cell division. The supplementation of oocytes with mtDNA in combination with fertilisation improves the maintenance of the expression of several key developmental genes and the patterns of parental allele-specific imprinting gene expression in blastocysts. These results suggest associations between mtDNA deficiency and meiotic cell cycle and the developmental effects of mtDNA supplementation on Sus scrofa blastocysts.
Melatonin Treatments Reduce Chilling Injury and Delay Ripening, Leading to Maintenance of Quality in Cherimoya Fruit
Authors: Medina-Santamarina Jorge , Guillén Fabián , Ilea Mihaela Iasmina Madalina , Ruiz-Aracil María Celeste , Valero Daniel , Castillo Salvador , Serrano María
Published: February 2023

Spain is the world’s leading producer of cherimoya, a climacteric fruit highly appreciated by consumers. However, this fruit species is very sensitive to chilling injury (CI), which limits its storage. In the present experiments, the effects of melatonin applied as dipping treatment on cherimoya fruit CI, postharvest ripening and quality properties were evaluated during storage at 7 °C + 2 days at 20 °C. The results showed that melatonin treatments (0.01, 0.05, 0.1 mM) delayed CI, ion leakage, chlorophyll losses and the increases in total phenolic content and hydrophilic and lipophilic antioxidant activities in cherimoya peel for 2 weeks with respect to controls. In addition, the increases in total soluble solids and titratable acidity in flesh tissue were also delayed in melatonin-treated fruit, and there was also reduced firmness loss compared with the control, the highest effects being found for the 0.05 mM dose. This treatment led to maintenance of fruit quality traits and to increases in the storage time up to 21 days, 14 days more than the control fruit. Thus, melatonin treatment, especially at 0.05 mM concentration, could be a useful tool to decrease CI damage in cherimoya fruit, with additional effects on retarding postharvest ripening and senescence processes and on maintaining quality parameters. These effects were attributed to a delay in the climacteric ethylene production, which was delayed for 1, 2 and 3 weeks for 0.01, 0.1 and 0.05 mM doses, respectively. However, the effects of melatonin on gene expression and the activity of the enzymes involved in ethylene production deserves further research.
Taurine and Creatine Transporters as Potential Drug Targets in Cancer Therapy
Authors: Stary Dorota , Bajda Marek
Published: February 2023

Cancer cells are characterized by uncontrolled growth, proliferation, and impaired apoptosis. Tumour progression could be related to poor prognosis and due to this fact, researchers have been working on novel therapeutic strategies and antineoplastic agents. It is known that altered expression and function of solute carrier proteins from the SLC6 family could be associated with severe diseases, including cancers. These proteins were noticed to play important physiological roles through transferring nutrient amino acids, osmolytes, neurotransmitters, and ions, and many of them are necessary for survival of the cells. Herein, we present the potential role of taurine (SLC6A6) and creatine (SLC6A8) transporters in cancer development as well as therapeutic potential of their inhibitors. Experimental data indicate that overexpression of analyzed proteins could be connected with colon or breast cancers, which are the most common types of cancers. The pool of known inhibitors of these transporters is limited; however, one ligand of SLC6A8 protein is currently tested in the first phase of clinical trials. Therefore, we also highlight structural aspects useful for ligand development. In this review, we discuss SLC6A6 and SLC6A8 transporters as potential biological targets for anticancer agents.
Gain-of-Function p53N236S Mutation Drives the Bypassing of HRasV12-Induced Cellular Senescence via PGC–1α
Authors: Yang Hao , Zhang Ke , Guo Yusheng , Guo Xin , Hou Kailong , Hou Jing , Luo Ying , Liu Jing , Jia Shuting
Published: February 2023

One of the key steps in tumorigenic transformation is immortalization in which cells bypass cancer-initiating barriers such as senescence. Senescence can be triggered by either telomere erosion or oncogenic stress (oncogene-induced senescence, OIS) and undergo p53- or Rb-dependent cell cycle arrest. The tumor suppressor p53 is mutated in 50% of human cancers. In this study, we generated p53N236S (p53S) mutant knock-in mice and observed that p53S heterozygous mouse embryonic fibroblasts (p53S/+) escaped HRasV12-induced senescence after subculture in vitro and formed tumors after subcutaneous injection into severe combined immune deficiency (SCID) mice. We found that p53S increased the level and nuclear translocation of PGC–1α in late-stage p53S/++Ras cells (LS cells, which bypassed the OIS). The increase in PGC–1α promoted the biosynthesis and function of mitochondria in LS cells by inhibiting senescence-associated reactive oxygen species (ROS) and ROS-induced autophagy. In addition, p53S regulated the interaction between PGC–1α and PPARγ and promoted lipid synthesis, which may indicate an auxiliary pathway for facilitating cell escape from aging. Our results illuminate the mechanisms underlying p53S mutant-regulated senescence bypass and demonstrate the role played by PGC–1α in this process.
Knockout of Purinergic P2Y6 Receptor Fails to Improve Liver Injury and Inflammation in Non-Alcoholic Steatohepatitis
Authors: Nishiyama Kazuhiro , Ariyoshi Kohei , Nishimura Akiyuki , Kato Yuri , Mi Xinya , Kurose Hitoshi , Kim Sang Geon , Nishida Motohiro
Published: February 2023

Nonalcoholic steatohepatitis (NASH) is a disease that progresses from nonalcoholic fatty liver (NAFL) and which is characterized by inflammation and fibrosis. The purinergic P2Y6 receptor (P2Y6R) is a pro-inflammatory Gq/G12 family protein-coupled receptor and reportedly contributes to intestinal inflammation and cardiovascular fibrosis, but its role in liver pathogenesis is unknown. Human genomics data analysis revealed that the liver P2Y6R mRNA expression level is increased during the progression from NAFL to NASH, which positively correlates with inductions of C-C motif chemokine 2 (CCL2) and collagen type I α1 chain (Col1a1) mRNAs. Therefore, we examined the impact of P2Y6R functional deficiency in mice crossed with a NASH model using a choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD). Feeding CDAHFD for 6 weeks markedly increased P2Y6R expression level in mouse liver, which was positively correlated with CCL2 mRNA induction. Unexpectedly, the CDAHFD treatment for 6 weeks increased liver weights with severe steatosis in both wild-type (WT) and P2Y6R knockout (KO) mice, while the disease marker levels such as serum AST and liver CCL2 mRNA in CDAHFD-treated P2Y6R KO mice were rather aggravated compared with those of CDAHFD-treated WT mice. Thus, P2Y6R may not contribute to the progression of liver injury, despite increased expression in NASH liver.
Structure Fault Tolerance of Bubble-Sort Star Graphs
Authors: You Lantao , Jiang Jianfeng , Han Yuejuan
Published: February 2023

As two significant performance indicators, structure connectivity and substructure connectivity have been widely studied, and they are used to judge a network’s fault tolerance properties from the perspective of the structure becoming faulty. An n-dimensional bubble-sort star graph BSn is a popular interconnection network with many good properties. We find the upper bounds of κ(BSn;K1,3) and κs(BSn;K1,3) in this paper. Furthermore, we establish κ(BSn;H) and κs(BSn;H) of BSn, where H∈{K1,K1,1,K1,2}.
Novel Light Convolutional Neural Network for COVID Detection with Watershed Based Region Growing Segmentation
Authors: Khan Hassan Ali , Gong Xueqing , Bi Fenglin , Ali Rashid
Published: February 2023

A rapidly spreading epidemic, COVID-19 had a serious effect on millions and took many lives. Therefore, for individuals with COVID-19, early discovery is essential for halting the infection’s progress. To quickly and accurately diagnose COVID-19, imaging modalities, including computed tomography (CT) scans and chest X-ray radiographs, are frequently employed. The potential of artificial intelligence (AI) approaches further explored the creation of automated and precise COVID-19 detection systems. Scientists widely use deep learning techniques to identify coronavirus infection in lung imaging. In our paper, we developed a novel light CNN model architecture with watershed-based region-growing segmentation on Chest X-rays. Both CT scans and X-ray radiographs were employed along with 5-fold cross-validation. Compared to earlier state-of-the-art models, our model is lighter and outperformed the previous methods by achieving a mean accuracy of 98.8% on X-ray images and 98.6% on CT scans, predicting the rate of 0.99% and 0.97% for PPV (Positive predicted Value) and NPV (Negative predicted Value) rate of 0.98% and 0.99%, respectively.
Study on Elucidation of the Roundness Improvement Mechanism of the Internal Magnetic Abrasive Finishing Process Using a Magnetic Machining Tool
Authors: Liu Jiangnan , Zou Yanhua
Published: February 2023

The magnetic abrasive finishing process using the magnetic machining tool was proposed to finish the internal surface of the thick tube (the thickness of the tube is 5~30 mm). It has been proved that this process can improve the roundness while improving the roughness. In this paper, we mainly study the machining mechanism of roundness improvement. Firstly, the influence of finishing characteristics on the roundness improvement was discussed, including the rotational speed of the magnetic machining tool and the rotational speed of the tube. It was concluded that the roundness improvement increases with the increase in the rotational speed through the analysis of finishing force and finishing times. Furthermore, the influence on roundness improvement of different distributions of magnetic particles were experimentally compared. After finishing, due to the magnetic force generated by the magnetic machining tool and the magnetic pole unit exerting pressure on the magnetic particles, a fixed magnetic brush is formed. The experimental results show that the circumferential length of the fixed magnetic brush is different due to the different distribution areas of magnetic particles. It was concluded that the roundness improvement increases with the circumferential length of the fixed magnetic brush increases by discussing the relationship between the circumferential length of the fixed magnetic brush and the wavelength of the roundness curve. When the circumferential length of the fixed magnetic brush is 76 mm, the roundness was improved from 379 μm to 236 μm after 60 min of finishing.
Monitoring the Rice Panicle Blast Control Period Based on UAV Multispectral Remote Sensing and Machine Learning
Authors: Ma Bin , Cao Guangqiao , Hu Chaozhong , Chen Cong
Published: February 2023

The heading stage of rice is a critical period for disease control, such as for panicle blast. The rapid and accurate monitoring of rice growth is of great significance for plant protection operations in large areas for mobilizing resources. For this paper, the canopy multispectral information acquired continuously by an unmanned aerial vehicle (UAV) was used to obtain the heading rate by inversion. The results indicated that the multi-vegetation index inversion model is more accurate than the single-band and single-vegetation index inversion models. Compared with traditional inversion algorithms such as neural network (NN) and support vector regression (SVR), the adaptive boosting algorithm based on ensemble learning has a higher inversion accuracy, with a correlation coefficient (R2) of 0.94 and root mean square error (RMSE) of 0.12 for the model. The study suggests that a more effective inversion model of UAV multispectral remote sensing and heading rate can be built using the AdaBoost algorithm based on the multi-vegetation index, which provides a crop growth information acquisition and processing method for determining the timing of rice tassel control.
Accelerated Thermo-Catalytic Degradation of Perfluoropolyether (PFPE) Lubricants for Space Applications
Authors: Gleirscher Milena , Wolfberger Archim , Schlögl Sandra , Hołyńska Małgorzata , Hausberger Andreas
Published: February 2023

Perfluoropolyethers (PFPE) are a class of frequently used lubricants in space applications due to their high stability under demanding conditions. However, they are susceptible to aging, with the aging mechanism being dependent on the specific material combination and storage condition. A Lewis-acid-induced thermo-catalytic degradation mechanism is of concern, for example, under steel-on-steel sliding contact, and can be relevant for long-term storage (LTS). Accelerated aging experiments were performed on Fomblin® Z25 and Krytox™ 143AC to investigate thermal stability under the influence of iron(III) fluoride (FeF3) at elevated temperatures (180 °C, 200 °C, and 220 °C) up to a total duration of 2000 h. The degradation effects were monitored via selected analysis techniques: mass loss of the samples due to degradation and subsequent evaporation during aging, FTIR spectroscopy to investigate changes to the chemical structure, dynamic viscosity measurements for the investigation of a potential impact due to changes in molecular mass, and a ball-on-disc tribological test setup to obtain friction behavior of the aged lubricants. Distinct differences between the two types of PFPE lubricants regarding stability to thermo-catalytic degradation were found. Fomblin® Z25 was highly affected by the presence of FeF3 within the selected aging conditions, exhibiting high mass loss, a significant drop in dynamic viscosity, and an increased coefficient of friction due to degradation reactions.