bluuebunny/crossref_metadata_2025 huggingface dataset:
Microbial Ecology of Greek Wheat Sourdoughs, Identified by a Culture-Dependent and a Culture-Independent Approach
Authors: Syrokou Maria K. , Themeli Christina , Paramithiotis Spiros , Mataragas Marios , Bosnea Loulouda , Argyri Anthoula A. , Chorianopoulos Nikos G. , Skandamis Panagiotis N. , Drosinos Eleftherios H.
Published: November 2020

The aim of the present study was to assess the microecosystem of 13 homemade spontaneously fermented wheat sourdoughs from different regions of Greece, through the combined use of culture-dependent (classical approach; clustering by Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) and identification by PCR species-specific for Lactiplantibacillus plantarum, and sequencing of the 16S-rRNA and 26S-rRNA gene, for Lactic Acid Bacteria (LAB) and yeasts, respectively) and independent approaches [DNA- and RNA-based PCR-Denaturing Gradient Gel Electrophoresis (DGGE)]. The pH and Total Titratable Acidity (TTA) values ranged from 3.64–5.05 and from 0.50–1.59% lactic acid, respectively. Yeast and lactic acid bacteria populations ranged within 4.60–6.32 and 6.28–9.20 log CFU/g, respectively. The yeast: LAB ratio varied from 1:23–1:10,000. A total of 207 bacterial and 195 yeast isolates were obtained and a culture-dependent assessment of their taxonomic affiliation revealed dominance of Lb. plantarum in three sourdoughs, Levilactobacillus brevis in four sourdoughs and co-dominance of these species in two sourdoughs. In addition, Companilactobacillusparalimentarius dominated in two sourdoughs and Fructilactobacillussanfranciscensis and Latilactobacillus sakei in one sourdough each. Lactococcus lactis, Lb. curvatus, Leuconostoc citreum, Ln. mesenteroides and Lb. zymae were also recovered from some samples. Regarding the yeast microbiota, it was dominated by Saccharomyces cerevisiae in 11 sourdoughs and Pichia membranifaciens and P. fermentans in one sourdough each. Wickerhamomyces anomalus and Kazachstania humilis were also recovered from one sample. RNA-based PCR-DGGE provided with nearly identical results with DNA-based one; in only one sample the latter provided an additional band. In general, the limitations of this approach, namely co-migration of amplicons from different species to the same electrophoretic position and multiband profile of specific isolates, greatly reduced resolution capacity, which resulted in only partial verification of the microbial ecology detected by culture-dependent approach in the majority of sourdough samples. Our knowledge regarding the microecosystem of spontaneously fermented Greek wheat-based sourdoughs was expanded, through the study of sourdoughs originating from regions of Greece that were not previously assessed.
Synthesis, Characterization, and Evaluation of Genistein-Loaded Zein/Carboxymethyl Chitosan Nanoparticles with Improved Water Dispersibility, Enhanced Antioxidant Activity, and Controlled Release Property
Authors: Xiao Yu , Ho Chi-Tang , Chen Yulian , Wang Yuanliang , Wei Zihao , Dong Mingsheng , Huang Qingrong
Published: November 2020

Genistein is one of major isoflavones derived from soybean products and it is believed to have beneficial effects on human health. However, its low water-solubility and poor oral bioavailability severely hamper its use as a functional food ingredient or for pharmaceutical industry. In this study, zein and zein/carboxymethyl chitosan (CMCS) nanoparticles were prepared to encapsulate genistein using a combined liquid–liquid phase separation method. The physicochemical properties of fabricated nanoparticles were characterized by dynamic light scattering (DLS), atomic force microscopy (AFM), and Fourier transform infrared spectroscopy (FTIR). The results demonstrated that genistein encapsulated with zein nanoparticles significantly improved its water dispersibility, antioxidant activity in the aqueous phase, and photostability against UV light. Moreover, genistein encapsulated in zein nanoparticles showed a sustained release property. Furthermore, it was found that encapsulation efficiency of genistein was significantly enhanced after CMCS coating, and this effect was more pronounced after the complex nanoparticles cross-linked with calcium ions when compared with the use of zein as a single encapsulant. In addition, compared to zein nanoparticles without biopolymer coating, CMCS coating significantly enhanced the thermal and storage stability of the formed nanoparticles, and delayed the release of genistein. A schematic diagram of zein and zein/carboxymethyl chitosan (CMCS) nanoparticles formation mechanism for encapsulation of genistein was proposed. According to the results of the current study, it could be concluded that encapsulation of genistein in zein/CMCS nanoparticles is a promising approach to improve its water dispersibility, antioxidant activity, photostability against UV light and provide controlled release for food/pharmaceutical applications.
Preliminary Study of Microelements, Phenolics as well as Antioxidant Activity in Local, Homemade Wines from North-East Greece
Authors: Skendi Adriana , Papageorgiou Maria , Stefanou Stefanos
Published: November 2020

The present study is aimed to analyze the composition of microelements in wines prepared by amateur winemakers in Drama region (recognized as Protected Geographical Indication (PGI)), especially the toxic ones, as well as the phenolic content and antioxidant activity of these wines since moderate wine consumption may contribute to health benefits to the consumers. A total of twenty-four wines, comprising sixteen red and eight white, were tested. The micro and macroelements were determined with ICP-OES (Inductively coupled plasma-optical emission spectrometry). Chromatic characteristics of wines and total phenolic and flavonoid compounds as well as their antioxidant activity with three different assays were determined spectrophotometrically. The amateur’s wines showed levels of contaminants with toxic metals less than the limits set from the International Organization of Vine and Wine (OIV). Moreover, toxic metals concentrations were comparable to those of European wines. The Principal Component Analysis discriminated wines into white and red and further distinguished the red wine into two groups depending on the Total Flavonoid content, antioxidant activity and color intensity. The moderate consumption of amateur wines can be recommended since their consumption is not harmful to the health, and they contain high amount of phenolics and flavonoids comparable or even higher to that of commercial wines.
Unraveling the Genome-Wide Impact of Recombinant Baculovirus Infection in Mammalian Cells for Gene Delivery
Authors: Shin Ha Youn , Choi Hanul , Kim Nahyun , Park Nayoung , Kim Heesun , Kim Jaebum , Kim Young Bong
Published: November 2020

Baculovirus expression systems have been widely used to produce recombinant mammalian proteins owing to the lack of viral replication in vertebrates. Although several lines of evidence have demonstrated impacts of baculovirus infection in mammalian hosts, genome-wide effects have not been fully elucidated. Here, we provide comparative transcriptome profiles of baculovirus and host-immune response genes in recombinant baculovirus-infected mammalian and insect cells. Specifically, to decipher the impacts of baculovirus infection in mammalian cells, we conducted total RNA-seq on human 293TT cells and insect Sf9 cells infected with recombinant baculovirus. We found that baculovirus genes were rarely expressed under the control of baculoviral promoters in 293TT cells. Although some baculovirus early genes, such as PE38 and IE-01, showed limited expression in 293TT cells, baculoviral late genes were mostly silent. We also found modest induction of a small number of mammalian immune response genes associated with Toll-like receptors, cytokine signaling, and complement in baculovirus-infected 293TT cells. These comprehensive transcriptome data will contribute to improving recombinant baculovirus as tools for gene delivery, gene therapy, and vaccine development.
Lymphatic Metastasis of NSCLC Involves Chemotaxis Effects of Lymphatic Endothelial Cells through the CCR7–CCL21 Axis Modulated by TNF-α
Authors: Zhang Shuai , Wang Hongzheng , Xu Zhiyun , Bai Yongkang , Xu Lin
Published: November 2020

Metastasis and recurrence are the main causes of lung adenocarcinoma patients’ death. Lymphatic metastasis is the main way of non-small cell lung cancer (NSCLC) metastasis. C-C chemokine receptor type 7 (CCR7) overexpression has been demonstrated to mediate occurrence and progression of NSCLC. Moreover, Chemokine ligand 21 (CCL21) was used to activate CCR7. The CCR7–CCL21 axis is one of the most common “chemokine-receptor” modes of action in the development and metastasis of multiple tumors. However, the role of the CCR7–CCL21 axis in lymphatic metastasis of NSCLC is poorly understood. The study was conducted to investigate the molecular mechanism underlying CCR7–CCL21 axis-mediated lymphatic metastasis of NSCLC A549 cells. Tumor necrosis factor α (TNF-α) could regulate the tumor microenvironment balance by promoting chemokine secretion. Our study demonstrated that TNF-α promoted CCL21 production in human lymphatic endothelial cells (HLEC). Results further showed that TNF-α significantly activated the NF-κB pathway in HLEC. NF–κB pathway inhibition with ammonium pyrrolidinedithiocarbamate (PDTC) caused a significant decrease in CCL21 secretion, suggesting that TNF-α-induced CCL21 secretion in HLEC was through NF–κB pathway. Co-culture of A549 cells and TNF-α-treated HLEC confirmed that the metastasis of A549 cells was enhanced, meanwhile, apoptosis-related proteins were hardly affected. The data proved that a co-culture system prevented cell apoptosis while inducing the lymphatic metastasis of A549 cells. However, the situation was reversed after neutralizing CCL21 expression, suggesting that TNF-α-induced CCL21 secretion in HLEC is involved in A549 cells metastasis. Collectively, our finding demonstrated that NF-κB pathway-controlled CCL21 secretion of HLEC contributing to the lymphatic metastasis of A549 cells via the CCR7–CCL21 axis, validating the CCR7–CCL21 axis as a potential target to inhibit metastasis of NSCLC.
Factors Determining the Choice of a Career in Geriatrics among Students in Geriatric in-Hospital Training: A Prospective Study of 74 Medical Students
Authors: Nuss Valentine , Barben Jérémy , Laborde Caroline , Vovelle Jérémie , Deidda Martha , Mihai Anca-Maria , Putot Alain , Manckoundia Patrick
Published: November 2020

To understand why students in the 2nd cycle of medical studies choose to complete a Diploma of Specialized Studies (DSS) in geriatrics, we conducted a study to identify the factors influencing the choice of a future specialty. In addition, we assessed the impact of clinical in-hospital training (CIHT) in a geriatric hospital on the students’ selection of their future specialty. We included all students who completed CIHT in the geriatric facility of our University Hospital between 1 May and 31 October 2018. Data were collected using a two-part questionnaire: one part was given before CIHT and the other after. The students were classified into two groups: those considering a career in geriatrics (CIG) before CIHT, forming the group DSS geriatrics+ (GDSSG+), and those not considering it, constituting the group DSS geriatrics− (GDSSG−). Seventy-four students aged 22 years old were included. Of these students, 26% were considering a CIG before CIHT. This rate increased significantly to 42% after CIHT (p = 0.04). However, none of the students who indicated that they were potentially interested in pursuing geriatrics before CIHT preselected geriatrics as their first option. For more than 92% of the students, the comprehensive care of geriatric patients was an asset. The main drawbacks were diagnostic and therapeutic limitations (60% of students), then managing aging, disability, and neurocognitive disorders (55% of students). After CIHT, the view of geriatrics improved by 74%. In conclusion, geriatric CIHT improves students’ opinions of geriatrics and increases the number of students considering a CIG. However, geriatrics still suffers from a lack of prestige.
Knowledge, Attitude, and Barriers to Telerehabilitation-Based Physical Therapy Practice in Saudi Arabia
Authors: Aloyuni Saleh , Alharbi Raed , Kashoo Faizan , Alqahtani Mazen , Alanazi Ahmad , Alzhrani Msaad , Ahmad Mehrunnisha
Published: November 2020

(1) Telerehabilitation (TR) is a part of telemedicine involved in providing rehabilitation services to people in remote locations. TR in physical therapy in the kingdom of Saudi Arabia is still in its infancy and its implementation may pose different challenges in the physical therapy settings. The purpose of this nation-wide survey is to explore physiotherapists (PTs) knowledge, attitudes, and barriers towards implementation of TR in physical therapy settings; (2) Methods: A 14 item questionnaire was developed and mailed to PTs working in hospitals and rehabilitation centers across 13 provinces in Saudi Arabia; (3) Results: 347 PTs responded. Results are as follows: 58.8% (n = 204) of PTs reported that they had sufficient knowledge about TR. About31.7% (n = 110) of PTs reported that their hospital and rehabilitation center had installed TR, yet only 19.9% (n = 69) utilized the TR facility. Image-based TR was more frequently used (n = 33) as compared to sensor-based TR (n = 29) and virtual reality TR (n = 10).The main barriers were technical issues and cost related to implement TR in physical therapy settings; and (4) Conclusions: There is a relatively high number of PTs with self-reported knowledge about TR, however facilities and usage were limited. The main barriers were technical issues, staff skills, and the high cost involved in the introduction of TR in the PT-based health care settings.
Reply to “Comment on the Correlation between Complete Blood Count Parameters and Appendix Diameter for the Diagnosis of Acute Appendicitis”
Authors: Daldal Emin , Dagmura Hasan
Published: November 2020

We would like to thank Akbulut and his colleague for reading our article with interest, and for their valuable comments and criticisms [...]
Women’s Empowerment as a Mitigating Factor for Improved Antenatal Care Quality despite Impact of 2014 Ebola Outbreak in Guinea
Authors: Merrell Laura K. , Blackstone Sarah R.
Published: November 2020

Improving maternal outcomes and reducing pregnancy morbidity and mortality are critical public health goals. The provision of quality antenatal care (ANC) is one method of doing so. Increasing women’s empowerment is associated with positive women’s health outcomes, including the adequate timing and amount of ANC use. However, little is known about the relationship between women’s empowerment and quality ANC care. Despite a history of political instability, low women’s equality and poor maternal health, the Republic of Guinea has committed to improving the status of women and access to health. However, the 2014 Ebola outbreak may have had a negative impact on achieving these goals. This study sought to examine factors in the relationship between women’s empowerment and the receipt of quality ANC (indicated by the number of health components) within the context of the Ebola outbreak. This study conducted multiple logistic regressions examining associations between covariates and the number of ANC components received using data from the 2012 and 2018 Guinea Demographic Health Surveys. Several aspects of women’s empowerment (healthcare decision-making, literacy/access to magazines, monogamous relationship status, contraceptive use, socio-economic status/employment) were significantly linked with the receipt of a greater number of ANC components, highlighting the importance of women’s empowerment in accessing quality maternity care.
Facilitators and Inhibitors of Lifestyle Modification and Maintenance of KOREAN Postmenopausal Women: Revealing Conversations from FOCUS Group Interview
Authors: Kim Hye-Ryoung , Yang Hwa-Mi
Published: November 2020

Modifiable lifestyle behaviors, such as lack of physical activity, smoking, and unhealthy diet, are associated with the risk of cardiovascular diseases in postmenopausal women, in addition to other risk factors, such as aging and physiological changes. Therefore, it is necessary to emphasize the importance of encouraging healthy lifestyles and health-promoting behaviors among postmenopausal women, to achieve a better health status. However, it is difficult to modify lifestyle and maintain that change. This study was aimed at identifying the factors that affect the maintenance of healthy lifestyle habits in postmenopausal women, using various theoretical models. This qualitative study included focus-group interviews with 21 Korean postmenopausal women aged 54 to 69 years. A theory-guided thematic analysis was performed based on the Health Belief Model, Self-Determination Theory, Social Cognitive Theory, and Theory of Planned Behavior. As a result, facilitators and inhibitors to healthy lifestyle modification and maintenance are identified. Various sources of motivation and reinforcement are important for menopausal women to maintain a healthy lifestyle. Autonomy support and self-regulation strategies play an important role in integrating health-promoting behaviors into a daily routine. In addition to personal effort, a social support system is also important to help individuals maintain a healthy lifestyle
Elusive Diagnostic Markers for Russian Wheat Aphid Resistance in Bread Wheat: Deliberating and Reviewing the Status Quo
Authors: Tolmay Vicki L. , Sydenham Scott L. , Sikhakhane Thandeka N. , Nhlapho Bongiwe N. , Tsilo Toi J.
Published: November 2020

Russian wheat aphid, Diuraphis noxia (Kurdjumov), is a severe pest of wheat, Triticum aestivum L., throughout the world. Resistant cultivars are viewed as the most economical and environmentally viable control available. Studies to identify molecular markers to facilitate resistance breeding started in the 1990s, and still continue. This paper reviews and discusses the literature pertaining to the D. noxia R-genes on chromosome 7D, and markers reported to be associated with them. Individual plants with known phenotypes from a panel of South African wheat accessions are used as examples. Despite significant inputs from various research groups over many years, diagnostic markers for resistance to D. noxia remain elusive. Factors that may have impeded critical investigation, thus blurring the accumulation of a coherent body of information applicable to Dn resistance, are discussed. This review calls for a more fastidious approach to the interpretation of results, especially considering the growing evidence pointing to the complex regulation of aphid resistance response pathways in plants. Appropriate reflection on prior studies, together with emerging knowledge regarding the complexity and specificity of the D. noxia–wheat resistance interaction, should enable scientists to address the challenges of protecting wheat against this pest in future.
Cellular Reprogramming—A Model for Melanoma Cellular Plasticity
Authors: Granados Karol , Poelchen Juliane , Novak Daniel , Utikal Jochen
Published: November 2020

Cellular plasticity of cancer cells is often associated with phenotypic heterogeneity and drug resistance and thus remains a major challenge for the treatment of melanoma and other types of cancer. Melanoma cells have the capacity to switch their phenotype during tumor progression, from a proliferative and differentiated phenotype to a more invasive and dedifferentiated phenotype. However, the molecular mechanisms driving this phenotype switch are not yet fully understood. Considering that cellular heterogeneity within the tumor contributes to the high plasticity typically observed in melanoma, it is crucial to generate suitable models to investigate this phenomenon in detail. Here, we discuss the use of complete and partial reprogramming into induced pluripotent cancer (iPC) cells as a tool to obtain new insights into melanoma cellular plasticity. We consider this a relevant topic due to the high plasticity of melanoma cells and its association with a strong resistance to standard anticancer treatments.
Extracellular Vesicles and the Oviduct Function
Authors: Harris Emily A. , Stephens Kalli K. , Winuthayanon Wipawee
Published: November 2020

In mammals, the oviduct (or the Fallopian tube in humans) can be divided into the infundibulum (responsible for oocyte pick-up), ampulla (site of fertilization), isthmus (where preimplantation embryos develop), and uterotubal junction (where embryos transit to the uterus). The oviductal fluid, as well as extracellular vesicles produced from the oviduct epithelial cells, referred to as oEVs, have been shown to improve the fertilization process, prevent polyspermy, and aid in embryo development. oEVs contain molecular cargos (such as miRNAs, mRNAs, proteins, and lipids) that can be delivered and fuse to recipient cells. oEVs produced from the ampulla appear to be functionally distinct from those produced from the isthmus. In multiple species including mice, cats, dogs, pigs, and cows, oEVs can be incorporated into the oocytes, sperm, and embryos. In this review, we show the positive impact of oEVs on gamete function as well as blastocyst development and how they may improve embryo quality in in vitro conditions in an assisted reproductive technology setting for rodents, domestic animals, farm animals, and humans.
miR-34a: A Promising Target for Inflammaging and Age-Related Diseases
Authors: Raucci Angela , Vinci Maria Cristina
Published: November 2020

The term “inflammaging” describes the chronic, low-grade systemic inflammation that occurs during physiological aging in the absence of an overt infection [...]
Random Forest with Sampling Techniques for Handling Imbalanced Prediction of University Student Depression
Authors: Sawangarreerak Siriporn , Thanathamathee Putthiporn
Published: November 2020

In this work, we propose a combined sampling technique to improve the performance of imbalanced classification of university student depression data. In experimental results, we found that combined random oversampling with the Tomek links under sampling methods allowed generating a relatively balanced depression dataset without losing significant information. In this case, the random oversampling technique was used for sampling the minority class to balance the number of samples between the datasets. Then, the Tomek links technique was used for undersampling the samples by removing the depression data considered less relevant and noisy. The relatively balanced dataset was classified by random forest. The results show that the overall accuracy in the prediction of adolescent depression data was 94.17%, outperforming the individual sampling technique. Moreover, our proposed method was tested with another dataset for its external validity. This dataset’s predictive accuracy was found to be 93.33%.
Use of Deep Learning to Study Modeling Deterioration of Pavements a Case Study in Iowa
Authors: Hosseini Seyed Amirhossein , Alhasan Ahmad , Smadi Omar
Published: November 2020

This paper describes the process and outcome of deterioration modeling for three different pavement types (asphalt, concrete, and composite) in the state of Iowa. Pavement condition data is collected by the Iowa Department of Transportation (DOT) and stored in a Pavement-Management Information System (PMIS). In the state of Iowa, the overall pavement condition is quantified using the Pavement Condition Index (PCI), which is a weighted average of indices representing different types of distress, roughness, and deflection. Deterioration models of PCI as a function of time were developed for the different pavement types using two modeling approaches. The first approach is the long/short-term memory (LSTM), a subset of a recurrent neural network. The second approach, used by the Iowa DOT, is developing individual regression models for each section of the different pavement types. A comparison is made between the two approaches to assess the accuracy of each model. The results show that the LSTM model achieved a higher prediction accuracy over time for all different pavement types.
The Function of LmPrx6 in Diapause Regulation in Locusta migratoria Through the Insulin Signaling Pathway
Authors: Chen Jun , Cui Dong-Nan , Ullah Hidayat , Li Shuang , Pan Fan , Xu Chao-Min , Tu Xiong-Bing , Zhang Ze-Hua
Published: November 2020

Peroxiredoxins (Prxs), which scavenge reactive oxygen species (ROS), are cysteine-dependent peroxide reductases that group into six structurally discernable classes: AhpC-Prx1, BCP-PrxQ, Prx5, Prx6, Tpx, and AhpE. A previous study showed that forkhead box protein O (FOXO) in the insulin signaling pathway (ISP) plays a vital role in regulating locust diapause by phosphorylation, which can be promoted by the high level of ROS. Furthermore, the analysis of transcriptome between diapause and non-diapause phenotypes showed that one of the Prxs, LmPrx6, which belongs to the Prx6 class, was involved. We presumed that LmPrx6 might play a critical role in diapause induction of Locusta migratoria and LmPrx6 may therefore provide a useful target of control methods based on RNA interference (RNAi). To verify our hypothesis, LmPrx6 was initially cloned from L. migratoria to make dsLmPrx6 and four important targets were tested, including protein-tyrosine phosphorylase 1B (LmPTP1B), insulin receptor (LmIR), RAC serine/threonine-protein kinase (LmAKT), and LmFOXO in ISP. When LmPrx6 was knocked down, the diapause rate was significantly reduced. The phosphorylation level of LmPTP1B significantly decreased while the phosphorylation levels of LmIR, LmAKT, and LmFOXO were significantly increased. Moreover, we identified the effect on two categories of genes downstream of LmFOXO, including stress tolerance and storage of energy reserves. Results showed that the mRNA levels of catalase and Mn superoxide dismutase (Mn-SOD), which enhanced stress tolerance, were significantly downregulated after silencing of LmPrx6. The mRNA levels of glycogen synthase and phosphoenolpyruvate carboxy kinase (PEPCK) that influence energy storage were also downregulated after knocking down of LmPrx6. The silencing of LmPrx6 indicates that this regulatory protein may probably be an ideal target for RNAi-based diapause control of L. migratoria.
Thermal Transformation of End-of-Life Latex to Valuable Materials
Authors: Haque Enamul , Pahlevani Farshid , Gorjizadeh Narjes , Hossain Rumana , Sahajwalla Veena
Published: November 2020

Recent studies have demonstrated that carbon nanomaterials have huge potential in composite applications, but there is a continuous quest for identifying the most viable technique for producing this material. In this study, the possibility of using an innovative approach for the synthesis of value-added carbon nanomaterials and green gases from end-of-life soft mattress materials (latex) was investigated. Thermogravimetric analysis (TGA) was used to determine the thermal degradation of latex. Quantitative gas analysis at three different temperatures by infrared spectroscopy (IR) suggested that small gas molecules, especially CH4, could be produced at a higher temperature. The carbon residues produced after gas analysis were characterised by XRD, SEM, TEM, XPS, and Raman spectroscopy, suggesting the possibility of direct synthesis of carbon nanomaterials from waste latex. These carbon materials have Na, Zn, Si, and K in their structure, and further study is needed for understanding the effect of these elements on composite properties. Our study demonstrated that heat treatment of waste latex at 1000 °C for 15 min produced carbon materials, which contained 7–16% S and 1.2–2% N, and gases, such as CH4, could be synthesised.
Assessing Rhotic Production by Bilingual Spanish Speakers
Authors: Cummings Ruiz Laura D. , Montrul Silvina
Published: November 2020

Due to its articulatory precision, the Spanish rhotic system is generally acquired in late childhood by monolingually-raised (L1) Spanish speakers. Heritage speakers and second language (L2) learners, unlike L1 speakers, risk an incomplete acquisition of the rhotic system due to limited Spanish input and possible phonological interference from English. In order to examine the effects of age of onset of bilingualism and cross-linguistic influence on bilinguals’ rhotic productions, twenty-four adult participants (six sequential bilingual heritage speakers, six simultaneous bilingual heritage speakers, six L1 Spanish speakers, six L2 Spanish learners) were audio recorded in a storytelling task and a picture naming task. The alveolar taps [ɾ] and alveolar trills [r] produced in these tasks were examined according to duration of the rhotic sound and number of apical occlusions. Results showed that the sequential bilinguals, but not the simultaneous bilinguals or the L2 learners, patterned similarly to the L1 Spanish speakers in their production of taps and trills. Neither heritage group produced the English alveolar approximant [ɹ]; the L2 learners, on the other hand, did produce [ɹ] when speaking Spanish. The results of this study suggest that early language input can affect the production of sounds that are acquired in late childhood.
Numerical Computation of Lightly Multi-Objective Robust Optimal Solutions by Means of Generalized Cell Mapping
Authors: Hernández Castellanos Carlos Ignacio , Schütze Oliver , Sun Jian-Qiao , Morales-Luna Guillermo , Ober-Blöbaum Sina
Published: November 2020

In this paper, we present a novel algorithm for the computation of lightly robust optimal solutions for multi-objective optimization problems. To this end, we adapt the generalized cell mapping, originally designed for the global analysis of dynamical systems, to the current context. This is the first time that a set-based method is developed for such kinds of problems. We demonstrate the strength of the novel algorithms on several benchmark problems as well as on one feed-back control design problem where the objectives are given by the peak time, the overshoot, and the absolute tracking error for the linear control system, which has a control time delay. The numerical results indicate that the new algorithm is well-suited for the reliable treatment of low dimensional problems.
A Memetic Decomposition-Based Multi-Objective Evolutionary Algorithm Applied to a Constrained Menu Planning Problem
Authors: Marrero Alejandro , Segredo Eduardo , León Coromoto , Segura Carlos
Published: November 2020

Encouraging healthy and balanced diet plans is one of the most important action points for governments around the world. Generating healthy, balanced and inexpensive menu plans that fulfil all the recommendations given by nutritionists is a complex and time-consuming task; because of this, computer science has an important role in this area. This paper deals with a novel constrained multi-objective formulation of the menu planning problem specially designed for school canteens that considers the minimisation of the cost and the minimisation of the level of repetition of the specific courses and food groups contained in the plans. Particularly, this paper proposes a multi-objective memetic approach based on the well-known multi-objective evolutionary algorithm based on decomposition (MOEA/D). A crossover operator specifically designed for this problem is included in the approach. Moreover, an ad-hoc iterated local search (ILS) is considered for the improvement phase. As a result, our proposal is referred to as ILS-MOEA/D. A wide experimental comparison against a recently proposed single-objective memetic scheme, which includes explicit mechanisms to promote diversity in the decision variable space, is provided. The experimental assessment shows that, even though the single-objective approach yields menu plans with lower costs, our multi-objective proposal offers menu plans with a significantly lower level of repetition of courses and food groups, with only a minor increase in cost. Furthermore, our studies demonstrate that the application of multi-objective optimisers can be used to implicitly promote diversity not only in the objective function space, but also in the decision variable space. Consequently, in contrast to the single-objective optimiser, there was no need to include an explicit strategy to manage the diversity in the decision space in the case of the multi-objective approach.
Inventory Management at a Chilean Hospital Pharmacy: Case Study of a Dynamic Decision-Aid Tool
Authors: Silva-Aravena Fabián , Ceballos-Fuentealba Irlanda , Álvarez-Miranda Eduardo
Published: November 2020

Pharmacy inventory management is a critical process in healthcare centers. On the one hand, effective drug procurement is fundamental for fulfilling the therapeutic requirements of patients. On the other hand, as hospital pharmacies’ purchasing and storage costs comprise an important share in the hospital budgets, efficient inventory management may play a central role in operational cost containment. Therefore, healthcare centers should design and implement decision-aid strategies for planning the purchase of drugs with the aim of avoiding excessive purchasing volumes and optimizing warehouse capacity, while also meeting forecast demand and ensuring critical stock levels. In this study, we present the methodological features of a decision-aid tool for planning the purchases and inventory levels for the controlled medication pharmacy of the Regional Hospital of Talca, Chile. We report the results obtained after 1 year of operation; these results show that our strategy produced more than 7% savings compared to the regular inventory planning strategy and was more effective in preserving critical stock levels. Furthermore, from a computational point of view, our strategy outperforms a recently published approach for a similar application.
Some Intrinsic Properties of Tadmor–Tanner Functions: Related Problems and Possible Applications
Authors: Kyurkchiev Nikolay
Published: November 2020

In this paper, we study some properties of an exponentially optimal filter proposed by Tadmor and Tanner. More precisely, we consider the problem for approximating the function of rectangular type F(t) by the class of exponential functions σadapt(t) about the Hausdorff metric. We prove upper and lower estimates for “saturation”—d (in the case q=2). New activation and “semi-activation” functions based on σadapt(t) are defined. Some related problems are discussed. We also consider modified families of functions with “polynomial variable transfer”. Numerical examples, illustrating our results using CAS MATHEMATICA are given.
Characterizations, Potential, and an Implementation of the Shapley-Solidarity Value
Authors: Su Jun , Liang Yuan , Wang Guangmin , Xu Genjiu
Published: November 2020

In this paper, we provide cooperative and non-cooperative interpretations of the Shapley–Solidarity value for cooperative games with coalition structure. Firstly, we present two new characterizations of this value based on intracoalitional quasi-balanced contributions property. Secondly, we study a potential function of the Shapley–Solidarity value. Finally, we propose a new bidding mechanism for the Solidarity value and then apply the result to the Shapley–Solidarity value.
Culture-Dependent Microbiome of the Ciona intestinalis Tunic: Isolation, Bioactivity Profiling and Untargeted Metabolomics
Authors: Utermann Caroline , Echelmeyer Vivien A. , Blümel Martina , Tasdemir Deniz
Published: November 2020

Ascidians and their associated microbiota are prolific producers of bioactive marine natural products. Recent culture-independent studies have revealed that the tunic of the solitary ascidian Cionaintestinalis (sea vase) is colonized by a diverse bacterial community, however, the biotechnological potential of this community has remained largely unexplored. In this study, we aimed at isolating the culturable microbiota associated with the tunic of C.intestinalis collected from the North and Baltic Seas, to investigate their antimicrobial and anticancer activities, and to gain first insights into their metabolite repertoire. The tunic of the sea vase was found to harbor a rich microbial community, from which 89 bacterial and 22 fungal strains were isolated. The diversity of the tunic-associated microbiota differed from that of the ambient seawater samples, but also between sampling sites. Fungi were isolated for the first time from the tunic of Ciona. The proportion of bioactive extracts was high, since 45% of the microbial extracts inhibited the growth of human pathogenic bacteria, fungi or cancer cell lines. In a subsequent bioactivity- and metabolite profiling-based approach, seven microbial extracts were prioritized for in-depth chemical investigations. Untargeted metabolomics analyses of the selected extracts by a UPLC-MS/MS-based molecular networking approach revealed a vast chemical diversity with compounds assigned to 22 natural product families, plus many metabolites that remained unidentified. This initial study indicates that bacteria and fungi associated with the tunic of C.intestinalis represent an untapped source of putatively new marine natural products with pharmacological relevance.
Convalescent Plasma against COVID-19: A Broad-Spectrum Therapeutic Approach for Emerging Infectious Diseases
Authors: Thijssen Marijn , Devos Timothy , Ejtahed Hanieh-Sadat , Amini-Bavil-Olyaee Samad , Pourfathollah Ali Akbar , Pourkarim Mahmoud Reza
Published: November 2020

In the lack of an effective vaccine and antiviral treatment, convalescent plasma (CP) has been a promising therapeutic approach in past pandemics. Accumulating evidence in the current severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic corroborates the safety of CP therapy and preliminary data underline the potential efficacy. Recently, the Food and Drug Administration (FDA) permitted CP therapy for coronavirus disease 2019 (COVID-19) patients under the emergency use authorization, albeit additional clinical studies are still needed. The imminent threat of a second or even multiple waves of COVID-19 has compelled health authorities to delineate and calibrate a feasible preparedness algorithm for deploying CP as an immediate therapeutic intervention. The success of preparedness programs depends on the interdisciplinary actions of multiple actors in politics, science, and healthcare. In this review, we evaluate the current status of CP therapy for COVID-19 patients and address the challenges that confront the implementation of CP. Finally, we propose a pandemic preparedness framework for future waves of the COVID-19 pandemic and unknown pathogen outbreaks.
Weak Influence of Paleoenvironmental Conditions on the Subsurface Biosphere of Lake Ohrid over the Last 515 ka
Authors: Thomas Camille , Francke Alexander , Vogel Hendrik , Wagner Bernd , Ariztegui Daniel
Published: November 2020

Lacustrine sediments are widely used to investigate the impact of climatic change on biogeochemical cycling. In these sediments, subsurface microbial communities are major actors of this cycling but can also affect the sedimentary record and overprint the original paleoenvironmental signal. We therefore investigated the subsurface microbial communities of the oldest lake in Europe, Lake Ohrid (North Macedonia, Albania), to assess the potential connection between microbial diversity and past environmental change using 16S rRNA gene sequences. Along the upper ca. 200 m of the DEEP site sediment record spanning ca. 515 thousand years (ka), our results show that Atribacteria, Bathyarchaeia and Gammaproteobacteria structured the community independently from each other. Except for the latter, these taxa are common in deep lacustrine and marine sediments due to their metabolic versatility adapted to low energy environments. Gammaproteobacteria were often co-occurring with cyanobacterial sequences or soil-related OTUs suggesting preservation of ancient DNA from the water column or catchment back to at least 340 ka, particularly in dry glacial intervals. We found significant environmental parameters influencing the overall microbial community distribution, but no strong relationship with given phylotypes and paleoclimatic signals or sediment age. Our results support a weak recording of early diagenetic processes and their actors by bulk prokaryotic sedimentary DNA in Lake Ohrid, replaced by specialized low-energy clades of the deep biosphere and a marked imprint of erosional processes on the subsurface DNA pool of Lake Ohrid.
Zinc Gallium Oxide—A Review from Synthesis to Applications
Authors: Chen Mu-I , Singh Anoop Kumar , Chiang Jung-Lung , Horng Ray-Hua , Wuu Dong-Sing
Published: November 2020

Spinel ZnGa2O4 has received significant attention from researchers due to its wide bandgap and high chemical and thermal stability; hence, paving the way for it to have potential in various applications. This review focuses on its physical, optical, mechanical and electrical properties, contributing to the better understanding of this material. The recent trends for growth techniques and processing in the research and development of ZnGa2O4 from bulk crystal growth to thin films are discussed in detail for device performance. This material has excellent properties and is investigated widely in deep-ultraviolet photodetectors, gas sensors and phosphors. In this article, effects of substrate temperature, annealing temperature, oxygen partial pressure and zinc/gallium ratio are discussed for device processing and fabrication. In addition, research progress and future outlooks are also identified.
Understanding the Fate of Almond (Prunus dulcis (Mill.) D.A. Webb) Oleosomes during Simulated Digestion
Authors: Trombetta Domenico , Smeriglio Antonella , Denaro Marcella , Zagami Roberto , Tomassetti Mara , Pilolli Rosa , De Angelis Elisabetta , Monaci Linda , Mandalari Giuseppina
Published: November 2020

Background: Almond kernels contain phytochemicals with positive health effects in relation to heart disease, diabetes and obesity. Several studies have previously highlighted that almond cell wall encapsulation during digestion and particle size are factors associated with these benefits. In the present study, we have characterized almond oleosomes, natural oil droplets abundant in plants, and we have investigated their integrity during simulated gastrointestinal digestion. Methods: Oleosomes were visualized on the almond seed surface by imaging mass spectrometry analysis, and then characterized in terms of droplet size distribution by dynamic light scattering and protein profile by liquid chromatography high-resolution tandem mass spectrometry analysis. Results: The almond oleosomes’ distribution remained monomodal after in vitro mastication, whereas gastric and duodenal digestion led to a bimodal distribution, albeit characterized mainly by a prevalent population with a droplet size decrease related to a rearrangement of the protein profile. Oleosins, structural proteins found in plant oil bodies, persisted unchanged during simulated mastication, with the appearance of new prunin isoforms after gastric and duodenal digestion. Conclusions: The rearrangement of the protein profile could limit lipid bioaccessibility. The data improve our understanding of the behavior of almond lipids during gastrointestinal digestion, and may have implications for energy intake and satiety imparted by almonds.
Whey Versus Casein as a Protein Source during the Weaning Period: Impact on Growth and Later Adiposity and Glucose Homeostasis in a Rat Model of Intrauterine Growth Restriction
Authors: Shahkhalili Yasaman , Blancher-Budin Florence , Monnard Cathriona , Moulin Julie , Sanchez-Garcia José , Macé Katherine
Published: November 2020

The impact of early life protein source (whey vs. casein) on short- and long-term glucose homeostasis and adiposity is unknown and was investigated in this study. At the end of the suckling period, non-IUGR (intrauterine growth restriction) and IUGR pups were separated from dams and were randomized into four groups. From age 21–49 days, non-IUGR and IUGR pups were fed ad-libitum chow or a semi-synthetic diet (20% from protein; casein or whey) and from age 50–199 days, all groups were fed ad-libitum chow. Food intake, body composition, glucose, and insulin homeostasis were assessed. Among the chow groups, IUGR had slower growth and higher fasting glucose at age 42 days, as well as higher fasting and AUC glucose at age 192 days relative to non-IUGR. The whey IUGR group had a slower growth rate and higher fasting glycemia in early life (age 21–49 days) and higher HOMA-IR later in life (age 120–122 and 190–192 days) relative to casein IUGR. This study shows the potential advantage of casein relative to whey during weaning on short term energy intake, growth, and glucose homeostasis in an IUGR model and reveals, for the first time, its long term impact on insulin sensitivity, which may have implications for later metabolic health, particularly in small-for-gestational-age populations at risk of type 2 diabetes.
Diet Influences the Oral Microbiota of Infants during the First Six Months of Life
Authors: Oba Patrícia M. , Holscher Hannah D. , Mathai Rose Ann , Kim Juhee , Swanson Kelly S.
Published: November 2020

Background: Oral microorganisms contribute to oral health and disease, but few have studied how infant feeding methods affect their establishment. Methods: Infant (n = 12) feeding records and tongue and cheek swabs were collected within 48 h of birth, and after 2, 4, and 6 mo. DNA was extracted from samples, bacterial and fungal amplicons were generated and sequenced using Illumina MiSeq, and sequences were analyzed using Quantitative Insights Into Microbial Ecology (QIIME) and Statistical Analysis System (SAS) to evaluate differences over time and among breast-fed, formula-fed, mixed-fed, and solid food-fed infants. Results: Considering all time points, breast milk- and mixed-fed infants had lower oral species richness than solid food-fed infants (p = 0.006). Regardless of feeding mode, species richness was lower at birth than at other time points (p = 0.006). Principal coordinates analysis (PCoA) of unique fraction metric (UniFrac) distances indicated that bacterial communities were impacted by feeding method (p < 0.005). Considering all time points, breast-fed infants had higher Streptococcus, while formula-fed infants had higher Actinomyces and Prevotella. Regardless of feeding mode, Propionibacterium, Porphyromonas, Prevotella, Gemella, Granulicatella, Veillonella, Fusobacterium, Leptotrichia, Neisseria, and Haemophilus increased with age, while Cloacibacterium and Dechloromonas decreased with age. Oral fungi were detected in infants but were not impacted by diet. Conclusions: These findings demonstrate that the establishment of oral bacteria depends on dietary composition and age. More research is necessary to determine whether this affects risk of oral caries and other health outcomes later in life.