bluuebunny/crossref_metadata_2025 huggingface dataset:
Length-Weight Relationship and Condition Factor Channa Marulioides (Bleeker, 1851) In Kapuas River – West Kalimantan as a Conservation Aquatic Resources Effort
Authors: Hutagalung Rizal Akbar , Anggoro Sutrsino , Suryanti Suryanti , Muskananfola Max Rudolf
Published: March 2024

Channa marulioides is one of the species that is facing a population decrease due to high fishing activity. This study aims to determine the value of the length-weight relationship and condition factors of C. marulioides in the Kapuas River as a relevant conservation effort. Several sampling areas in this study were used to represent the Kapuas River, including Kapuas Hulu River in Kapuas Hulu Regency, Putussibau District; The central part of the waters area of ​​Sintang Regency in Sintang Regency; and in the downstream waters of Pontianak City. The study carried out the sampling by analyzing the criteria for specimens with a dimension weight > 500 grams and length > 30 cm, with the same amount for each sex. The results showed that the growth type in the upper and middle waters was positive allometric with a value of b 3.1; however, it is negative allometric in the middle and upstream waters with b values ​​of 2.5 and 1.7. After research, the condition factor of the upstream and middle waters was observed to be in good condition with a value of 1. However, in the downstream waters the condition of the fish was rather bad with a value below 1, namely 0.99. The study concludes there is a decrease in the quality of the aquatic environment and the food availability from the middle waters to the downstream. This is what underlines conservation efforts in the form of domestication activities within the scope of structured fish farming.
Prevalence, Intensity and Degree of Infestation Profile Argulus japonicus Ectoparasite in Common Carp (Cyprinus carpio) at Ngawi and Tambakrejo, Sidoarjo, East Java, Indonesia
Authors: Bahtiar Sadida Anindya , Kismiyati  , Nirattisai Petchsupa
Published: March 2024

The demand for common carp in Indonesia is high, and the success of seed production relies on the quality of the broodstock. However, freshwater aquaculture faces challenges from diseases, particularly parasites like Argulus japonicus. This study aimed to determine the prevalence, intensity, and degree of infestation of A. japonicus on common carp. The research method was survey. The research was conducted in October 2019 at Ngawi Fish Hatchery Center and in November 2023 at Tambakrejo Village, Waru, Sidoarjo, East Java, Indonesia. There were 26 broodstock of common carp as samples. A. japonicus were ectoparasites found to infest 10 common carp (Cyprinus carpio) broodstock that were ready to spawn at Fish Hatchery Center in Ngawi, East Java and 16 common carp (C. carpio) that raised from Tambakrejo Village farmer in Waru, Sidoarjo, East Java. Prevalence of the fish infested with A. japonicus was same in 100% at both regions. However, there is different in the intensity of A. japonicus at Ngawi Fish Hatchery Center (29 individuals/head) and at Tambakrejo Village, Waru, Sidoarjo (42,5 individuals/head). But, the degree of infestation in both location was classified as severe. Attachment site of A. japonicus were in the fish body surface, fins, and mouth. During the research, water quality is in normal limits that can be observed by 28°C temperature, 7.1 pH, and 2.6 mg/L DO. In conclusion, common carp in Ngawi Fish Hatchery Center and Tambakrejo Village, Waru, Sidoarjo, East Java, were infested with the heavy category of A. japonicus.
Zoonotic Ectoparasite Burden in House Rats (Rattus spp.) in Selected Urban and Rural Areas of NCR and CALABARZON
Authors: Chan Helenar , Flores Mary Jane , Maghirang Eligio Santiago , Arellano Bridget , Chan Jan Michael
Published: March 2024

Rats and other mammalian species co-inhabiting or living in proximity with humans can serve as potential reservoir hosts and may contribute to the transmission of parasitic infections. Aside from endoparasites, ectoparasites such as lice, mites, ticks, and fleas, can also serve as vectors of various pathogens inducing diseases and contaminating the environment. This study aimed to determine the prevalence rate of Polyplax spinulosa (spiny rat louse) infestation among house rats in selected urban and rural areas in the Philippines. A total of 60 Rattus spp. were captured and examined. The overall prevalence rate of Polyplax spinulosa was 71.7% (43/60). Of the infestations, 23.3% (14/60) were recorded in Metro Manila while 48.3% (29/60) were recorded in CALABARZON. These results have revealed that rodents like rats play an important role in both direct and indirect transmission of zoonotic diseases since these pests themselves could be host to a variety of diseases. As a result, there is an immediate need to provide education and raise awareness about the role of rodents as reservoir hosts and vectors of these zoonotic diseases to develop long-term strategies for controlling and preventing rodent populations and the transmission of zoonotic ectoparasite infestation.
Identification, Prevalence, and Infestation Patterns of Ectoparasitic In MP Hybrid Type Culled Laying Ducks In Gedang Sub Village, Modopuro Village, Mojosari Sub District, Mojokerto District
Authors: Aurandini Nailla Ridhani , Hastutiek Poedji , Utama Suzanita , Sunarso Agus , Suprihati Endang , Witaningrum Adiana Mutamsari
Published: March 2024

The purpose of this study was to identify ectoparasites and to determine the prevalence and infestation patterns of ectoparasites that attack the MP hybrid type culled ducks in Gedang Hamlet, Modopuro Village, Mojosari District, Mojokerto Regency. This study used a sample of 100 culled laying ducks of the MP hybrid type. The obtained ectoparasites were identified at the labaroratory of division parasitology, Faculty of veterinary medicine, Universitas Airlangga, Surabaya, using the permanent mounting method without staining and viewed using a microscope with 40-100x magnification. This study found 71 samples positively infested with ectoparasites out of 100 samples of culled MP hybrid ducks examined with (prevalence of 71%). The types of ectoparasites found in this study were Menacanthus stramineus (25%), Menopon gallinae (9%), and Anaticola crassicornis (59%). Correspondence analysis test was conducted to show the pattern of infestation of the ectoparasites. The head-neck region was infested by M. stramineus and M. gallinae, while the back, wings, abdomen and tail regions were infested by A. crassicornis. A. crassicornis mostly attacked the MP hybrid type of culled laying ducks on the wings, compared to the back, abdomen and tail. A. crassicornis is an ectoparasite that is typical for the wing region, while M. gallinae and M. stramineus are ectoparasites that are typical for the head-neck region.
CHARACTERISTICS OF ANOPHELES BREEDING PLACES IN DAHIAN TAMBUK VILLAGE, GUNUNG MAS DISTRICT, CENTRAL KALIMANTAN
Authors: Tasya Zahrawana Mouldy , Mutiasari Dian , Ratnasari Arini , Jabal Arif Rahman , Triawan Nopta , Kurniawan M. Yusuf Ilham , Djajanto Marchelia , Karmila Mila
Published: March 2024

Background: Malaria cases in Dahian Tambuk Village have been ranked first for four consecutive years in Gunung Mas District. Purpose: This study aims to describe the physical and chemical characteristics of Anopheles breeding places in Dahian Tambuk Village, Gunung Mas District. Method: It is a descriptive study using a survey method. The characteristics studied were Anopheles species, number, temperature, salinity, breeding sites, and density of larvae found in each breeding site. Result: Seven breeding places were found positive to contain Anopheles larvae, consisting of puddles, fish ponds, and sewers. The sewer was predominantly found. Physical characteristics of water showed a temperature ranging from 29.9 0C - 35 0C, pH levels between 5.35 - 6, and a salinity of 0 ppt, with a density level of 9 larvae/2 dippers. The species of Anopheles larvae found in all breeding sites was identified as Anopheles kochi. Conclusion: Seven breeding sites found in the studied area consisted of puddles, fish ponds, and sewers, with physical characteristics that qualify for Anopheles larvae in general An. kochi was the only species of larvae found in all breeding sites.
ANTIOXIDANT POTENTIAL OF TEMULAWAK (CURCUMA XANTHORRHIZA) EXTRACT GEL AS A CANDIDATE FOR WOUND HEALING
Authors: Malau Reghita Claudia , Nasution Sri Wahyuni , Nasution Ali Napiah , Widowati Wahyu , Salfa Nindya Faradhina , Kusuma Hanna Sari Widya
Published: March 2024

Background: The complete treatment of chronic wounds remains a significant unmet medical need. To expedite the healing of chronic wounds, numerous studies have begun to utilize gels, including hydrogels or sol-gels, which incorporate a combination of antioxidant properties. Curcuma xanthorrhiza is known to contain polyphenolic compounds, which include flavonoids acting as an antioxidant. Purpose: Examine the potential of C. xanthorrhiza extract (CXE) gel as a candidate for wound-healing by measuring its antioxidant activity. Method: Three CXE gel formulas were prepared from different concentrations of CMC-Na (3, 4, and 5%). Each formula consisted of 5% CXE, 15% propylene Glycol, 10% Glycerin, 0.25% Methylparaben, and distilled water. The quality of the CXE gel was tested through homogeneity, spreadability, pH, and viscosity tests. The antioxidant activity was measured by 2,2-Diphenyl-1-picrylhydrazil (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)-reducing activity assay in a gel formula that was close the standards. The measurement results were then used in the calculation of antioxidant activity to determine the IC50 value. Result: Formulas 1, 2, and 3 meet the requirements by yielding a homogeneous gel with a spreadability of 5.37 – 5.93 cm, a pH level of 5.87 – 6.10, and a viscosity of 8.800 – 9296.67 cps. The highest DPPH and ABTS percentages were 34.04% and 5.28%, respectively. The IC50 values of CXE gel in DPPH and ABTS were 1973.38 μg/mL and 700.65 μg/mL, respectively. Conclusion: The CXE 1, 2, and 3 gel formula meets the requirements and has the potential to be used as a wound healing therapy through its antioxidant properties.
Cervical opening during estrus in Aceh Local Cows based on camera-equipped artificial insemination endoscope
Authors: Komala Ulfa  , Hendra Saputra  , Tazul Arifin  , Cut Nila Thasmi  , Hafizuddin  , Etriwati  , Abdullah Hamzah
Published: March 2024

Twelve healthy and not pregnant local cows, aged 3-6 years, 1-3 parity. The cows were synchronized using the PGF2α of 5 ml/cow intramuscularly, twice at 11 days intervals. The Estrus detection based on the scoring of van Eerdenburg was conducted 24 hours after the second prostaglandin injection three times daily for three days. The changes in cervical dilatation were observed at 0, 6, and 12 hours from the onset of estrus. The result showed that 8 of 12 cows were estrus after twice PGF2α injection. All the estrus cows showed mild cervical dilatation with liquid and transparent mucus (opening cervix score 1) at 0-hour observation. Six hours later, 62.50% of cows remained at opening cervix score 1, and 37.50% changed to opening cervix score 2 (optimum cervical dilatation with thick and transparent mucus). At 12 hours after the onset of estrus, 25% of estrus cows had cervical closure (opening cervix score 0, closed cervical with thick and turbid mucus), and 75% changed to opening cervix score 2. In this study, 75% of the cows experienced an optimum open cervix at 12 hours from the onset of estrus. It could be concluded that opening the external cervical canal of local Aceh cows reaches optimum at 12 hours from the onset of estrus.
Intracervical Artificial Insemination using Frozen Semen of Boer buck on Pote Does at Different Times of Estrus
Authors: Susilowati Suherni , Tri Wahyu Suprayogi  , Tatik Hernawati  , Yossy Imam Candika  , Widya Paramita Lokapirnasari
Published: March 2024

Pote goats are kept by the rural community in Bangkalan Regency, Madura Island, Indonesia. Estrus symptoms in goats are usually unclear, causing low fertility. This study aimed to determine the intracervical artificial insemination at early and mid-estrus on the Pote does. The 24 the does were randomly divided into two groups and estrous synchronization with PGF2α of 200 µg intramuscularly, twice injections with 11 days intervals. The T1 groups of estrus the Pote does were inseminated at early estrus (0-12 hours). Meanwhile, T2 groups of estrus the Pote does were inseminated at mid-estrus (12-24 hours after signs of estrus appeared. The insemination conducted artificially using post-thawed Boer semen. The observed variables were non-return rate at day 21 post-insemination (NRR21), and pregnancy rate (PR) based on abdominal palpation diagnosis. The result showed that post-thawed Boer buck was qualified (sperm motility more than 40%) for artificial insemination. All of the Pote does were estrus after twice PGF2α intramuscular injection with 11 days intervals. The onset of estrus was 48-72 hours after the second injection PGF2α. The NRR21 and PR ranged from 66.66-100% and 37.50-66.66%, respectively. The NRR21 and PR of the T2 group were higher than those of the T1 group. It could be concluded that intracervical artificial insemination on Pote the does at mid-estrus were more effective, resulting in higher NRR21 and PR than those at early estrus.
Risk factors for dystocia in primiparous and pluriparous beef cows at the Besuki Animal Health Center, Situbondo Regency, East Java, Indonesia
Authors: Nisaaturrohma Fajriyah  , Soeharsono Soeharsono  , Oky Setyo Widodo
Published: March 2024

This study aims to determine the risk factors for dystocia in primiparous and pluriparous dams at the study site. Cases of dystocia, both maternal and fetal, were obtained as secondary data from records at the Situbondo Regency Animal Husbandry Service for year 2020 and 2021. Tracking on these secondary data was carried out to look for cows experienced dystocia and the owners’ names and addresses for interviews using a questionnaire worksheet. Interviews were conducted in February - May 2022 to obtain data on the maternal breed, age, feeding, fetal sex, and exercise frequency during pregnancy of the cows. Dystocia in the working area of the Besuki Animal Health Center in Sitobondo Regency in 2020 and 2021 occured in 50 cows which included primiparous (26%) and pluriparous (74%) cows. Breed, age, feed, and sex of the calves were not significantly different (p >0.05) between primiparous and pluriparous dams. However, the frequency of the exercises differed significantly (p <0.05) between primiparous and pluriparous dams. It could be concluded that the dystocia of cows in the study area was dominated by pluriparous parturition with exercises factor as the main cause.
Red dragon (Hylocereus polyrhizus) fruit peel extract increased the motility and viability of spermatozoa of hypercholesterolemic rats (Rattus norvegicus)
Authors: Niken Meyliana Sari  , M. Gandul Atik Yuliani  , Eduardus Bimo Aksono Herupradoto  , Nanik Hidayatik  , Nurhusien Yimer  , Tatik Hernawati
Published: March 2024

This study aims to determine the effect of red dragon (Hylocereus polyrhizus) fruit peel extract (RDFPE) on spermatozoa motility and viability of hypercholesterolemic rats (Rattus norvegicus) as a model. Twenty male rats were randomly divided into negative control (NC), positive control (PC), treatment 1 (T1), treatment 2 (T2), and treatment 3 (T3) groups. All rats were given 2 mL of high cholesterol feed orally every day for 28 days. On day-15, all rats were measured for their blood cholesterol levels, followed by treatment for 14 days. Rats in the NC, PC, T1, T2, and T3 groups were treated with 1% Na-CMC, Simvastatin 10 mg/kg BW, and RDFPE of 500, 750, and 1000 mg/kg BW, respectively. On day-29, all rats were sacrificed to evaluate spermatozoa viability and motility. The results showed that spermatozoa viability and motility in the hypercholesterolemic rats (NC) group were the lowest (p <0.05) among the groups. Treatment of hypercholesterolemic rats with Simvastatin 10 mg/kg BW (PC) group showed higher (p <0.05) spermatozoa viability and motility compared to the NC group. RDFPE dose of 1000 mg/kg BW (T3 group) resulted in higher (p <0.05) spermatozoa viability and motility compared to other RDFPE doses (T1 and T2) and the control (NC) groups, and it was similar (p >0.05) compared with the Simvastatin treated (PC) group. It could be concluded that the administration of 1000 mg/kg BW ethanolic extract of red dragon fruit (Hylocereus polyrhizus) peel increased the viability and motility of spermatozoa of hypercholesterolemic rats (Rattus norvegicus) which were the same as the group of rats given Simvastatin 10 mg/kg BW.
Time estimation for mating peak and calving of Bali cattle in semi-arid area in Netemnanu village Kupang district
Authors: Marianus Yakobus Magul Datur  , Tarsisius Considus Thopianong  , Cynthia Dewi Gaina  , Nancy Diana Federika Katarina Foeh
Published: March 2024

Bali cattle have advantages in terms of adaptation and fertilization rates, but tend to have slow growth rate because the rearing system is still grazing so that livestock growth becomes uncontrollable. This study aims to determine the peak of mating and calving estimation of Bali cattle (Bos sondaicus) that did not experience reproductive disorders, aged 4-7 years, belonging to smallholder farmers. The cattle were reared in semi-arid area of Netemnanu village, South Amfoang district, Kupang regency, Nusa Tenggara Timur. The method used was interviews with farmers, diagnosis of pregnancy through rectal palpation and ultrasonography. The results showed that the pregnancy rate was 86.20% (50/58).  Based on pregnancy data, it was known that the peak of mating would occur in December 2021 and estimated calving would reach its peak in September 2022.
Reproductive Performance of Crossbreed Cows on Lowland Terrain
Authors: Ardiyansyah Septiana Alafia Yusi , Sri Agus Sudjarwo  , Nusdianto Triakoso  , Tri Wahyu Suprayogi  , Ismudiono Ismudiono  , Oky Setyo Widodo
Published: March 2024

This study aims to identify the differences in reproduction performance of Ongole cross (PO), Limousine cross (Limpo), and Simmental cross (Simpo) in Kerek district, Tuban regency. Primary data was taken by interviewing inseminators and farmers, while secondary data was taken from the Fisheries and Animal Husbandry Department of Tuban regency. The total sample was 360 cows consisting of 30 PO, Limpo, and Simpo cows aged 3, 4, 5, and 6 years respectively which were calculated based on the Roscoe formula. Data was analyzed using the Simple Linear Regression and ANOVA followed by Duncan's multiple range test at a significance level of 95%. The results showed that the services per conception (S/C), conception rate (CR), and calving rate (CvR) were not significantly different (p >0.05) between PO, Limpo and Simpo cows. Meanwhile, days open (DO) and fertility status (FS) of PO cows were higher (p <0.05) than Limpo and Simpo cows. There was no significant difference (p >0.05) in DO and FS between Limpo and Simpo cows. The linear equation with age as a predictor (X) of S/C, DO, and CI (Y) values were not significant (p >0.05) in PO, Limpo, and Simpo cows. It could be concluded that the reproductive performance of PO cows was better than Limpo and Simpo cows.
Impact of green tea (Camellia sinensis) leaf extract in skim milk-goose egg yolk semen extender on the quality of Sapudi ram spermatozoa stored at 5°C
Authors: Ardina Sahra Miranda  , Tri Wahyu Suprayogi  , Budi Utomo  , Suherni Susilowati  , Yeni Dhamayanti
Published: March 2024

Livestock production requires Sapudi rams, a breed native to Indonesia, to meet meat demand and food security. In artificial high-quality frozen semen is needed to spread Sapudi rams. To maximize the survival of spermatozoa during cryopreservation, semen should be stored in an extender. Green tea leaf extract (GTLE) and skim milk-goat egg yolk (SM-GEY) may be a good cryoprotectants due to their antioxidant properties. This study aimed to determine the effect of adding GTLE to the SM-GEY extender on the quality of Sapudi ram spermatozoa stored at 5°C. The fresh semen sample was divided into four different GTLE treatment groups, which each contained a 0.1 mL semen sample and a 25-mL extender of SM-GEY. Group T0: no GTLE added to SM-GEY; Groups T1, T2, and T3: 0.1 mL semen diluted in 25 mL SM-GEY with 0.05, 0.10, and 0.15 mg GTLE. Extended semen was then stored at 5°C, and its quality was evaluated daily for five days. The variables observed included spermatozoa motility, viability, and membrane integrity. Data were analyzed using one-way analysis of variance followed by Duncan's test using Statistical Program and Service Solution version 23. The result of this study was that adding 0.05 mg GTLE to 25 mL of SM-GEY extender significantly maintained the spermatozoa motility, viability, and plasma membrane integrity of Sapudi ram spermatozoa for three days at 5°C (p <0.05). Therefore, it could be concluded that adding 0.05 mg of GTLE to the SM-GEY extender preserved Sapudi ram spermatozoa's motility, viability, and membrane integrity for three days at 5°C.
Reproduction record of captive Sumatera elephant (Elephas maximus sumatranus) at Way Kambas National Park, Indonesia
Authors: Sofie Adi Keren Jayanti , Budiarto Budiarto  , Hermin Ratnani  , Pudji Srianto  , Budi Utomo  , Suzanita Utama  , Setiawan Koesdarto  , Nurhusien Yimer
Published: March 2024

This research aims to determine reproductive data on Sumatran elephants (Elephas maximus sumatranus) in the ETC and ERU of Way Kambas National Park, Indonesia during 1988-2021. Data recorded from the elephant population at both locations (ETC and ERU) includes the number, gender of elephants, elephant calves, ages and birth dates. Calving intervals and service periods were calculated from calving records of cows with a minimum parity of two. Data collection produced data on 47 elephant calves from 13 female elephants with at least twice the parity and 12 primiparous cows. Elephant reproductive records at ETC and ERU were: age (37.44 ± 9.03 vs. 29.75 ± 3.30 years), parity (2.78 ± 1.09 vs. 2.50 ± 0.58), and age at first birth (18, 11 ± 3.92 vs. 17.75 ± 0.50 years). While the calving interval was 1857.56 ± 870.81 vs. 1833.00 ± 305.18 days, and service period respectively 1229.44 ± 846.18 vs. 1210.50 ± 283.59 days, respectively. It can be concluded that the calving interval and service period for captive elephants at ETC and ERU were not much different and were within the normal range. Young cows showed better reproductive efficiency than older cows. Monitoring calves is very important. Further assistance is needed to improve elephant mobile veterinary services, increase diagnostic laboratory capacity, and educate camp managers, veterinary assistants, and mahouts about elephant diseases, their monitoring and treatment. In addition, the use of reproductive technology such as monitoring ovulation using ultrasound and carrying out artificial insemination was expected to increase reproductive efficiency.
Addition of dextrose and caffeine to the intravenous treatment of milk fever in Holstein Friesian cattle
Authors: Ady Kurnianto  , Novianto Meska Pratama
Published: March 2024

A farmer reported a cow unable to stand 48 hours postpartum. The cow was a 2.5 years old dairy cow with a body weight of around 350 kg. The cow was fed elephant grass, standard concentrate (16–18%) crude protein, and drinking water ad libitum without mineral supplementation during the dry season. The cow's position and posture when lying down, ear temperature, and eye pupils were included in the physical examination. Availability of food and drink was examined to predict appetite and thirst. The results of the history and physical examination showed that the cow was unable to stand 48 hours after calving, had no appetite and eye reflexes, had cold ears, and trembling hind legs. The cow was laid on its right side, with its head turned to flank. Based on the anamnesis and physical examination, the cow was diagnosed as suffering from stage 2 puerperal hypocalcemia. The cow was infused via the jugular vein with a solution containing 270 mg calcium borogluconate, 70 mg magnesium borogluconate, 300 mg dextrose, 5 mg phosphorus element, 2 mg potassium chloride, and 5 mg caffeine per 1 mL of solution. Forty-five minutes after the infusion, the cow was able to stand, although it still looked weak. It could be concluded that the addition of dextrose and caffeine to the standard treatment of milk fever was beneficial for recovery.
CARGO TRANSPORT ON THE EXAMPLE OF A SELECTED MODE OF TRANSPORT IN POLAND
Authors: MACIOSZEK Elżbieta
Published: March 2024

The article presents the problem of the cargo transport with the example of a selected mode of transport in Poland. The volume of goods transported by air transport in 2010-2021 was analysed. Cargo transport in air transport is very popular. From year to year, there is an increase in the volume of transported goods. The coronavirus pandemic, and the related economic crisis did not significantly affect cargo transport, as was the case with passenger transport, the number of which decreased significantly during the pandemic.
WELDING OF 1.4462 DUPLEX STEEL USED IN MEANS OF ROAD TRANSPORT
Authors: SZCZUCKA-LASOTA Bożena , WĘGRZYN Tomasz
Published: March 2024

Materials with good mechanical and chemical properties (materials should be resistant to various types of corrosion) are proposed for the various structures of means of transport. For this reason, high-alloy steels, including duplex steels, are increasingly used in the automotive industry. The 1.4462 duplex steel used in means of road transport steel could be treated as an interesting high-strength stainless material used in the automotive industry. Generally, duplex steels have a high resistance to localized and uniform corrosion. The duplex and superduplex steels are assumed to be rather proper weldable materials; however, cracks could be observed both in the heat-affected zone (HAZ) and in the weld. The article analyses the influence of tubular cored metal arc (136) with active gas shield welding (process 136) parameters on the arrangement of correct joints used in tanker truck elements, as an example of welding in the automotive sector. Various mechanical tests checking the properties of joints were realized. The goal of the paper is to choose the correct welding parameters for elements of 1.4462 steel. The construction elements of a tanker truck are made by the 136 method of welding.
Equine Endothelial Cells Show Pro-angiogenic Behaviours in Response to FGF2 but Not VEGF-A
Authors: Finding Elizabeth JT , Faulkner Ashton , Wheeler-Jones Caroline PD
Published: March 2024

Understanding the factors which control endothelial cell (EC) function and angiogenesis is crucial for developing the horse as a disease model, but equine ECs remain poorly studied. In this study we have optimised methods for the isolation and culture of equine aortic endothelial cells (EA-oECs) and characterised their angiogenic functions in vitro. Mechanical dissociation, followed by magnetic purification using an anti-VE-cadherin antibody, resulted in EC-enriched cultures suitable for further study. Fibroblast growth factor 2 (FGF2) increased EAoEC proliferation rate and stimulated scratch wound closure and tube formation by EAoECs on extracellular matrix. Pharmacological inhibitors of FGFR1 (SU5402) or MEK (PD184352) blocked FGF2-induced ERK1/2 phosphorylation and functional responses, suggesting that these are dependent on FGFR1/MEK-ERK signalling. In marked contrast, VEGF-A had no effect on EAoEC proliferation, migration or tubulogenesis and did not promote ERK1/2 phosphorylation, indicating a lack of sensitivity to this classical pro-angiogenic growth factor. Gene expression analysis showed that, unlike human ECs, FGFR1 is expressed by EAoECs at a much higher level than both VEGFR1 and VEGFR2. These results suggest a predominant role for FGF2 versus VEGF-A in controlling the angiogenic functions of equine ECs. Collectively, our novel data provide a sound basis for stud-ying angiogenic processes in the horse and lay the foundations for comparative studies of EC biology in horses versus humans.
Mathematics of Epidemics: On the General Solution of SIRVD, SIRV, SIRD and SIR Compartment Models
Authors: Schlickeiser Reinhard , Kröger Martin
Published: March 2024

The susceptible-infected-recovered-vaccinated-deceased (SIRVD) epidemic compartment model extends the SIR model to include the effects of vaccination campaigns and time-dependent fatality rates on epidemic outbreaks. It encompasses the SIR, SIRV, SIRD, and SI models as special cases, with individual time-dependent rates governing transitions between different fractions. We investigate a special class of exact solutions and accurate analytical approximations for the SIRVD and SIRD compartment models. While the SIRVD and SIRD equations pose complex integro-differential equations for the rate of new infections and the fractions as a function of time, a simpler approach considers determining equations for the sum of ratios for given variations. This approach enables us to derive fully exact analytical solutions for the SIRVD and SIRD models. For nonlinear models with a high-dimensional parameter space, such as the SIRVD and SIRD models, analytical solutions, exact or accurately approximative, are of high importance and interest: not only as suitable benchmarks for numerical codes, but especially as they allow us to understand the critical behavior of epidemic outbursts as well as the decisive role of certain parameters. In the second part of our study, we apply a recently developed analytical approximation for the SIR and SIRV models to the more general SIRVD model. This approximation offers accurate analytical expressions for epidemic quantities, such as the rate of new infections and the fraction of infected persons, particularly when the cumulative fraction of infections is small. The distinction between recovered and deceased individuals in the SIRVD model affects the calculation of the death rate, which is proportional to the infected fraction in the SIRVD/SIRD cases but often proportional to the rate of new infections in many SIR models using a posteriori approach. We demonstrate that the temporal dependence of the infected fraction and the rate of new infections differs when considering the effects of vaccinations and when the real-time dependence of fatality and recovery rates diverge. These differences are highlighted for stationary ratios and gradually decreasing fatality rates. \Revised{The case of stationary ratios allows one to construct a new powerful diagnostics method to extract analytically all SIRVD model parameters from measured COVID-19 data of a completed pandemic wave.
Being Physically Active for a Long-Standing Mitigates Inflammaging Progression in Older Adults during the COVID-19 Pandemic
Authors: Dos Santos Carlos André Freitas , Nardy Ariane , Gomes Renato Jimenez , Silva Brenda Rodrigues , Monteiro Fernanda Rodrigues , Rossi Marcelo , Amaral Jônatas Bussador do , Paixão Vitória , Vaisberg Mauro , Amirato Gislene Rocha , Vieira Rodolfo de Paula , Dos Santos Juliana de Melo Batista , Furtado Guilherme Eustáquio , Ribeiro Ana Paula , Colombo Patrícia de Souza , Jacinto Alessandro Ferrari , Bachi André Luis Lacerda
Published: March 2024

Background: Here, we investigated the inflammaging and physical performance in older adults before and after the COVID-19 pandemic. Methods: Women(n=18) and men(n=7) older were evaluated before the COVID-19 pandemic(PRE), 12 months after the lockdown(POST), and 10 months after resuming exercise training(POS-TR). Physical tests [gait speed(GS) and timed-up-and-go(TUG)]; muscle strength (handgrip-HG), and serum cytokine levels were assessed. Results: The older women showed higher GS and TUG at POST than PRE and POST-TR, besides lower HG at POST-TR than PRE, whereas older men exhibited lower HG at POST and POST-TR than PRE. Both groups presented (1)lower IL-10 and IL-12p70 values in contrast to higher IL-6/IL-10 and IL-8/IL-10 ratios at POST than PRE; (2)higher IL-10 values and lower IL-8/IL-10 ratio at POST-TR than POST; (3)higher IL-12p70/IL-10 ratio at POST-TR than PRE and POST. Particularly older women showed: (4)lower IL-6 values at POST and POST-TR than PRE; (5)lower IL-8 and IL-10 values at POST-TR than POST; (6)and higher TNF-α/IL-10 and IFN-γ/IL-10 ratios at POST than PRE and POST-TR. Significant correlations between the variables were found in both groups. Conclusion: During the COVID-19 pandemic, detraining and resumption of exercise training promoted distinct alterations in physical capacity and inflammaging among older women and older men population.
Convergence in Discrete Inverse Dynamical Systems
Authors: Diedrich Eduardo
Published: March 2024

This article presents a new methodology for modeling and analyzing discrete dynamic systems through the construction of inverse algebraic models, giving rise to the Theory of Inverse Discrete Dynamical Systems. Key concepts such as inverse modeling, structural analysis in inverse algebraic trees, and the establishment of topological equivalences for the analytical transfer of properties between the canonical system and its inverted counterpart are introduced. Central theorems on homeomorphic invariance and topological transport are demonstrated, consolidating the validity of transferring cardinal attributes between both equivalent dynamic representations. As a concrete application, an alternative proof of the historic Collatz Conjecture is presented through the rigorous construction of the associated inverse model and the analytical transfer of properties exhibited in the inverted tree structure. Furthermore, evidence has been presented on Conway's Conjecture in the Game of Life through meticulous inverse modeling in bounded cases, overcoming initial limitations of the theory in combinatorial explosions. Although a complete proof of the conjecture is not achieved, this positively expands the modeling and analysis capacity of the methodology. The impact of properly applying this novel theory to expand understanding and solve open problems in discrete dynamical systems seems vast and profound.
A New Evidence Preservation Forensics Model Using Blockchain and Stenography Techniques
Authors: AlKhanafseh Mohammad , Surakhi Ola
Published: March 2024

The adoption of technology and digital tools across various user groups has brought about a heightened risk of cyber-attacks targeting devices and shared data. These elevated levels of cybercrime necessitate thorough investigations to address victims’ inquiries about the nature, causes, affected data, and impacted resources of the attack. Digital forensics serves as a crucial means to answer these queries. Additionally, it enables the formulation of strategies and delineation of roles essential for fortifying future defenses against such attacks. Within digital forensics, digital evidence holds pivotal importance in the investigative process as it serves as a cornerstone in legal proceedings to identify perpetrators. Numerous research endeavors focus on devising solutions to safeguard evidence, including centralized storage centers or cloud computing. These efforts aim to create frameworks integrating diverse security levels for sharing and preserving evidence effectively. In this paper, a new framework is proposed that seeks to amalgamate novel technologies with forensic practices to address challenges in the preservation phase of digital forensics. Strategies include segmenting evidence into smaller components, leveraging steganography to support preservation, and employing blockchain technology to ensure the integrity of collected evidence.
Enhanced Bone Healing in Critical-Sized Rabbit Femoral Defects: Impact of Helical and Alternate Scaffold Architectures.
Authors: Alonso Fernández Iván , Haugen Håvard Jostein , Nogueira Liebert Parreiras , López-Álvarez Miriam , González Pío , López-Peña Mónica , González-Cantalapiedra Antonio , Muñoz-Guzón Fernando
Published: March 2024

This study investigates the effect of scaffold architecture on bone regeneration, focusing on 3D-printed polylactic acid-bioceramic calcium phosphate (PLA-bioCaP) composite scaffolds in rabbit femoral condyle critical defects. To assess their influence on bone healing and scaffold performance, we explored two distinct scaffold designs, alternate and helical laydown patterns. The scaffolds were meticulously characterized for pore size, strut thickness, porosity, pore accessibility, and mechanical properties. The in vivo efficacy of these scaffolds was evaluated using a rabbit femoral condyle critical defect model. Our findings indicate that both scaffold architectures are biocompatible and support bone formation. The helical scaffolds, characterized by larger pore sizes and higher porosity, demonstrated significantly greater bone regeneration than the alternate structures. However, their lower mechanical strength presented limitations for use in load-bearing sites. This study highlights the importance of scaffold architecture in bone tissue engineering and underscores the need to balance porosity and mechanical strength for optimal bone regeneration. The findings provide crucial insights for the design of 3D- printed scaffolds in clinical applications, particularly in the context of critical-sized bone defects. The study also outlines potential areas for future research, including exploring hybrid scaffold designs to achieve optimal porosity without compromising mechanical integrity.
mRNA-Delivered Transcytosis Enabled Treatment of Neurodegenerative Diseases
Authors: Niazi Sarfaraz K. , Magoola Matthias
Published: March 2024

The recent setbacks in the withdrawal and approval delays of antibody treatments of neurodegenerative disorders (NDs) can be attributed to their poor entry across the blood-brain barrier (BBB). While several invasive and noninvasive approaches can be used to make the BBB more permeable, their inconsistency makes them a poor choice; however, if an antibody is chemically modified to attach a transferrin molecule that enhances its permeation across the BBB, this could be the most practical and useful modification that can transform the science of ND treatment. Antibodies can be conjugated with transferrin in vitro, but preferably through mRNA, as we propose to convert current treatments to bring faster treatment options. The mRNA technology yields products at a meager cost, which is a consideration for the affordability of these drugs globally. This paper provides complete details with templates to convert the current medicines and those under development into mRNA delivery systems to ensure clinical success, avoiding the current failures in their efficacy, more specifically the products that have demonstrated efficacy but are being removed from the market.
The Invasion Factor ODZ1 Is Upregulated through an Epidermal Growth Factor-Induced Pathway in Primary Glioblastoma Cells
Authors: Velasquez Carlos , Gutierrez Olga , Carcelén María , Fernandez-Luna Jose L.
Published: March 2024

We have previously shown that the transmembrane protein ODZ1 promotes cytoskeletal remodelling of glioblastoma (GBM) cells and invasion of the surrounding parenchyma through activation of a RhoA-ROCK pathway. We also described that GBM cells can control the expression of ODZ1 through transcriptional mechanisms triggered by binding of IL-6 to its receptor and a hypoxic environment. Epidermal growth factor (EGF) plays a key role in the invasive capacity of GBM. However, the molecular mechanisms that enable tumor cells to acquire the morphological changes to migrate out from the tumor core have not been fully characterized. Here we show that EGF is able to induce the expression of ODZ1 in primary GBM cells. We analysed the levels of EGF receptor (EGFR) in 20 GBM primary cell lines and found expression in 19 of them by flow cytometry. We selected two cell lines that do or do not express EGFR and found that EGFR-expressing cells responded to EGF ligand by increasing ODZ1 at the mRNA and protein level. Moreover, blockade of EGF-EGFR binding by Cetuximab, inhibition of the p38 MAPK pathway or siRNA-mediated knockdown of MAPK11 (p38 MAPK) reduced the induction of ODZ1 in response to EGF. Overall, we show that EGF may activate an EGFR-mediated signalling pathway through p38 MAPK, to upregulate the invasion factor ODZ1, which may initiate morphological changes for tumor cells to invade the surrounding parenchyma. These data identify a new candidate of the EGF-EGFR pathway for novel therapeutic approaches.
Willow (Salix acmophylla) Leaf and Branch Extracts Inhibit In Vitro Sporulation of Coccidia (Eimeria spp.) from Goats
Authors: Haj-Zaroubi Manal , Mattar Nariman , Awabdeh Sami , Sweidan Rawad , Markovics Alex , Klein Joshua D. , Azaizeh Hassan
Published: March 2024

Willow (Salix spp.) trees, found worldwide, contain secondary metabolites that are valuable as dietary supplements for animal feed and as anti-parasitic compounds. We quantified secondary metabolites (phenolic, flavonoids, and salicylic acid) in ethanolic extracts from leaves and branches of three Salix acmophylla genotypes and investigated their potential to inhibit Eimeria sp. sporulation, a major concern in ruminants. The total phenolic content of willow leaves and branches was similar in two of three different genotypes. The total flavonoid content of the branches was significantly higher than that of leaves of the same genotype; however, the salicylic acid content was significantly higher in leaves than in branches. Importantly, all extracts exhibited significant inhibition of Eimeria sporulation, where over 70% inhibition was obtained at concentrations as low as 750 ppm. The sporulation inhibition by branch or leaf extracts exceeded 80% for leaves and 90% for branches at concentrations above 1250 ppm. The study highlights the potential of using Salix extracts as bioactive compounds for biological control of coccidiosis in ruminants and emphasizes the importance of genotype-specific variations in secondary metabolite profiles. We conclude that all parts of S. acmophylla can provide secondary metabolites that act as a coccidiostat to treat Eimeria in goats.
An Efficient Trans Complementation System for In Vivo Replication of Defective Poliovirus Mutants
Authors: Arita Minetaro
Published: March 2024

The picornavirus genome encodes a large single polyprotein that is processed by viral proteases to form an active replication complex. The replication complex is formed with the viral genome, host proteins, and viral proteins that are produced/translated directly from each of the viral genomes (cis-provided viral proteins). Efficient complementation in vivo of replication complex formation by trans-provided viral proteins, thus exogenous or ectopically expressed viral proteins, remains to be demonstrated. Here we report an efficient trans-complementation system for the replication of defective poliovirus (PV) mutants by a viral polyprotein precursor in HEK 293 cells. Viral 3AB in the polyprotein, but not 2BC, was processed exclusively in cis. Replication of a defective PV replicon mutant, with a disrupted cleavage site for viral 3Cpro protease between 3Cpro and 3Dpol (3C/D[A/G] mutant) could be rescued by a trans-provided viral polyprotein. Only a defect of 3Dpol activity, of the replicon could be rescued in trans; inactivating mutations in 2CATPase/hel, 3B, and 3Cpro of the replicon completely abrogated the trans-rescued replication. An intact N-terminus of the 3Cpro moiety, of the trans-provided 3CDpro, was essential for the trans-rescue activity. By using this trans-complementation system, a high-titer defective PV pseudovirus (> 107 infectious units per mL) could be produced with the defective mutants, whose replication was completely dependent on trans-complementation. This work reveals potential roles of exogenous viral proteins in PV replication and offers insights into protein/protein and protein/RNA interactions during picornavirus infection.
Characterization of a Biodegradable Mg-Alloy after SLM Processing
Authors: Raducanu Doina , Cojocaru Vasile Danut , Nocivin Anna , Drob Silviu Iulian , Hendea Radu Emil , Stanciu Doina , Ivanescu Steliana , Raducanu Vlad Andrei , Serban Nicolae , Cojocaru Elisabeta Mirela , Campian Radu Septimiu
Published: March 2024

A new Mg-Zn-Zr-Ca alloy in powder state was obtained via mechanical alloying method, from pure elemental powder. Further, the obtained powder-alloy was processed by selective laser melting (SLM) procedure to obtain 3D printed samples for small biodegradable implants. A series of microstructural, mechanical and corrosion analysis were performed. The SEM analysis of powder-alloy reveal a good dimensional homogeneity, with uniform colored, no agglutinated and almost rounded particles, suitable for selective laser melting procedure. Further, the SLM samples reveal a robust and unbreakable morphology, with a suitable porosity and without undesirable balling effect. The tested Young’s modulus of the SLM samples, of 42 GPa, is close to cortical bone one, of 30 GPa. The corrosion tests performed in PBS (Phosphate-buffered saline) solution show that the corrosion parameters have a satisfactory evolution.
Inflammation Indexes PLR, SII, AISI, MLR, NLPR and MCVL in Peripartum Treated Thrombophilia Patients Undergoing Cesarean Section at Term-STROBE Compliant
Authors: Filip Catalina , Covali Roxana , Socolov Demetra , Vasilache Ingrid Andrada , Carauleanu Alexandru , Akad Mona , Scripcariu Ioana Sadyie , Butureanu Tudor , Dumachita-Sargu Gabriela , Boiculese Lucian Vasile , Melinte Alina , Socolov Razvan
Published: March 2024

(1) Background: Thrombophilia is a tendency towards hypercoagulability, and it is increased by pregnancy, peaking around delivery. Research question: does thrombophilia stimulate the in-flammatory state of the patient, already suppressed by pregnancy? (2) Methods: Eighty thrombo-philia patients admitted for delivery between 1/10/2017 and 1/12/2021 were prospectively stud-ied, together with eighty age- and para-matched control patients. Patients were admitted for de-livery at term by means of cesarean section. The inflammation indexes from the complete blood count values analysis during both the 24 hours before and after labor—extracted from the hospi-tal’s medical records—were calculated and then correlated with uterine involution. (3) Results: Except for the PLR, most of the numerous inflammation indexes studied were not significantly different in treated thrombophilia patients, before and after delivery, compared to non-thrombophilia patients. Only when we split the patients, based on the uterine haematometra in the first 24-48 hours postpartum, we found out that the few treated thrombophilia patients with haematometra (14 with haematometra out of 79 with treated thrombophilia) had elevated SII and AISI antepartum. In pregnant patients treated for thrombophilia, MLR is higher in pa-tients without the Rh factor than in those with the Rh factor. In non-thrombophilia patients, MCVL and NLPR increased with age and before labor, while SII and AISI increased with age postpartum. (4) Conclusions: Thrombophilia did not stimulate the inflammatory state of the pa-tient, already suppressed by pregnancy.