bluuebunny/crossref_metadata_2025 huggingface dataset:
Molecular Mechanism of Ethanol Fermentation Inhibition via Protein Tyrosine Nitration of Pyruvate Decarboxylase by Reactive Nitrogen Species in Yeast
Authors: Eknikom Supapid , Nasuno Ryo , Takagi Hiroshi
Published: January 2022

Protein tyrosine nitration (PTN), in which tyrosine (Tyr) residues on proteins are converted into 3-nitrotyrosine (NT), is one of the post-translational modifications mediated by reactive nitrogen species (RNS). Many recent studies have reported that PTN contributed to signaling systems by altering the structures and/or functions of proteins. This study aimed to investigate connections between PTN and the inhibitory effect of nitrite-derived RNS on fermentation ability using the yeast Saccharomyces cerevisiae. The results indicated that RNS inhibited the ethanol production of yeast cells with increased intracellular pyruvate content. We also found that RNS decreased the activities of pyruvate decarboxylase (PDC) as a critical enzyme involved in ethanol production. Our proteomic analysis revealed that the main PDC isozyme Pdc1 underwent the PTN modification at Tyr38, Tyr157, and Tyr344. The biochemical analysis using the recombinant purified Pdc1 enzyme indicated that PTN at Tyr157 or Tyr344 significantly reduced the Pdc1 activity. Interestingly, the substitution of Tyr157 or Tyr344 to phenylalanine, which is no longer converted into NT, recovered the ethanol production under the RNS treatment conditions. These findings suggest that nitrite impairs the fermentation ability of yeast by inhibiting the Pdc1 activity via its PTN modification at Tyr157 and Tyr344 of Pdc1.
Papaver Decaisnei: GC-MS Alkaloids Profiling, in Vitro Antioxidant, and Anticancer Activity
Authors: Aj.Jabbar Ahmed , Abdullah Fuad Othman , Abdulrahman Kamaran Kaiani , Galali yaseen , Sardar Abdullah Sh.
Published: January 2022

The Papaver L. plant have been well known as a source of pharmaceutically valuable alkaloids (noscapine, thebaine, codeine, roemerine, papaverine and morphine). The current study investigates the phytochemical, in-vitro antioxidant, and anticancer activities of papaver decaisnei, an endemic plant species to the flora of Kurdistan-Iraq. The chemical analysis of the methanolic (MeOH) extracts of flowers, leaves, and roots of papaver decaisnei were made by using gas chromatography-mass spectrophotometry (GC-MS), and the antioxidant activity evaluation done by radical scavenging [on 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2j-azino-bis (3- ethylbenzothiazoline-6-sulfonic acid) (ABTS)], and reducing power [cupric reducing antioxidant capacity (CUPRAC), and ferric reducing antioxidant power (FRAP)] assays. The anticancer actions were presented as IC50 (inhibitory concentration at 50%) on human colorectal adenocarcinoma (Caco-2), mammary cancer cells (MCF-7), and human cervical carcinoma (HeLa) cells. The results of the phytochemical analysis showed 17, 19, and 22 chemical compounds for flowers, leaves, and roots of P. decaisnei, respectively. The prevalent organic compounds of P. decaisnei were alkaloids, phenolics, fatty acids, esters, and phytosterols, namely Roemerine (70.44%), Decarbomethoxytabersonine, 9,12,15-Octadecatrien-1-ol, Hexadecanoic acid, 6,8-Dioxa-3-thiabicyclo(3,2,1)octane 3,3-dioxide, and γ-Sitosterol. The antioxidant activity of plant organ extracts was within 39.1-143.5 μg/ml for DPPH and 123.12-276.4 μg/ml for ABTS assays, while, the FRAP and CUPRAC values ranged within 12.4- 34.3 and 42.6-75.8 μg/ml, respectively. The anticancer action of P.decaisnei organ extracts was found against all tested human cell lines (Caco-2, MCF-7, HeLa) with inhibitory concentrations (IC50) values between 125.3-388.4 μg/ml. The presented data on alkaloid contents and biological activity of P. decaisnei can serve a ground knowledge for the future biomedical synthesis and cancer research projects.
Effect of Cultivars and Temperature on Synergistic Interaction Between Panicum Mosaic Virus and Satellite Panicum Mosaic Virus in Switchgrass
Authors: Muhle Anthony , Palmer Nathan , Edme Serge , Sarath Gautam , Yuen Gary , Mitchell Robert , Tatineni Satyanarayana
Published: January 2022

Panicum mosaic virus (PMV), the type species of the genus Panicovirus in the family Tombusviridae, naturally infects switchgrass (Panicum virgatum L.). PMV and its molecular partner, satellite panicum mosaic virus (SPMV), interact synergistically in co-infected millets with exacerbated disease phenotype and increased accumulation of PMV, compared to plants infected only by PMV. In this study, we examined the reaction of switchgrass cvs. Summer and Kanlow to PMV and PMV+SPMV infections at 24°C and 32°C. Switchgrass cv. Summer was susceptible to PMV at both temperatures. In contrast, cv. Kanlow was tolerant to PMV at 24°C but not at 32°C, suggesting that Kanlow harbors temperature-sensitive resistance against PMV. At 24°C, PMV was readily detected in inoculated leaves but not in upper non-inoculated leaves of Kanlow, suggesting that resistance to PMV was likely mediated by abrogation of long-distance virus transport. Co-infection by PMV and SPMV at 24°C and 32°C in cv. Summer but not in Kanlow caused increased symptomatic systemic infection and mild disease synergism with slightly increased PMV accumulation compared to plants infected only by PMV. These data suggest that the interaction between PMV and SPMV in switchgrass is cultivar dependent, manifested in Summer but not in Kanlow. However, co-inoculation of cv. Kanlow by PMV+SPMV caused an enhanced asymptomatic infection, suggesting a role for SPMV in enhancing symptomless infection in a tolerant cultivar. These data suggest that enhanced asymptomatic infections in virus-tolerant switchgrass cultivar could serve as a source for virus spread and play an important role in panicum mosaic disease epidemiology under field conditions. Our data revealed that cultivars, co-infection with SPMV, and temperature influenced the severity of symptoms elicited by PMV in switchgrass.
Inhibition of RANKL and Sema4d Improves Residual Ridge Resorption in Mice.
Authors: Hisamoto Meri , Kimura Shunsuke , Iwata Kai , Iwanaga Toshihiko , Yokoyama Atsuro
Published: January 2022

Residual ridge resorption (RRR) is a chronic and progressive bone resorption following tooth loss. It causes deterioration of the oral environments and leads to the pathogenesis of various systemic diseases. However, the molecular mechanisms and risk factors for RRR progression are still unclear and controversial. In this study, we developed a tooth extraction model using mice for analyzing long-term morphological and gene expression changes in the alveolar bone. We further applied ovariectomy to this model to elucidate the effects of osteoporosis on RRR progression. As a result, the alveolar bone loss was biphasic and consisted of rapid loss in the early stages and subsequently slow and sustained bone loss over a long period. Gene expression analysis indicated that ovariectomy increased the expression of pro-inflammatory cytokines in the alveolar bone and prolonged the activation of osteoclasts same as histological analysis. Furthermore, the expressions of Tnfsf11 and Sema4d kept increasing for a long time in OVX mice. Administration of neutralization antibodies for receptor activator of NF-κB ligand (RANKL) effectively suppressed RRR. Similarly, inhibition of Semaphorin 4d (Sema4d) also improved alveolar bone loss. This study demonstrated that osteoporosis is a risk factor for RRR and that RANKL and Sema4d suppression are potential treatments.
A Case-Control Study On Dietary Nutrition and Dietary Habits of Kazakh Esophageal Cancer Patients in Xinjiang
Authors: YIN Dong , YIN Yu , LI Siyao , DENG Yanchao , LI Xufeng , CHEN Yan
Published: January 2022

Objective To explore the causes of esophageal cancer (ESCC) and provide a theoretical basis for prevention and treatment of ESCC by analyzing dietary nutrients intake and other blood indicators of Kazakh patients with ESCC.Methods 198 ESCC cases and 200 controls were recruited in Xinjiang. The food frequency questionnaire was used to investigate the dietary nutrients intake. After collecting blood samples, the automated chemiluminescence immunoassay analyzer was applied to measure serum FA level and serum vitamin B12 level. The enzyme-linked immunosorbent assay was used to observe the serum DNMT1 level.Results The cholesterol intake of ESCC group was higher than that of the control group while the intakes of protein, thiamin, riboflavin, FA, vitamin A, B6, C and E were lower than the control group. Levels of serum FA and serum vitamin B12 in ESCC group were lower, while the serum DNMT1 level was higher.Conclusion Lacking fresh vegetables, low educational level, low income, alcoholism, eating solid and dry food and smoked meat, dieting irregularly, low serum FA level, high serum DNMT1 level were risky factors of ESCC for Kazakhs. The occurrence of ESCC reduced with the increase of serum FA but increased when serum DNMT1 increased.
New Paradigm On The Mechanical Properties of In-Situ Formed By Al/TiB2 and Al/TiB2/Cu MMCs
Authors: C Rajaravi , B Gobalakrishnan , A AMALA MITHIN MINTHER SINGH , P ARUL FRANCO , V SHARUN
Published: January 2022

In the current study, in-situ formed Al/6wt. % TiB2 and Al/6wt. % TiB2/4wt. % Cu Metal Matrix were investigated. Composites were made using the sir casting method, and both composites were compared. The composite is synthesized by combining two precursor salts, Potassium Hexa Fluro Titanate (KBF4) and potassium tetrafluoroborate (K2TiF6), with stoichiometric compositions corresponding to 6 percent by weight of TiB2 particles, with A356 aluminium melt at 820° C, speed 300 rpm, and holding time 30 minutes. Following that, 4wt. % Cu powder was added to the composite melt, which was then poured into the permanent mould. Mechanical properties tests such as tensile strength, hardness, and fracture toughness were carried out in accordance with ASTM guidelines. The mechanical properties of the in-situ formed Al/6wt. % TiB2/4wt. % Cu composite outperform those of the Al/6wt.% TiB2 composite and base metal. Optical micrograph and XRD analysis both confirm the presence of TiB2 and Cu particles.
Thermal Decomposition of 3-Nitro-1,2,4-Triazole-5-One (NTO) and Nanosize NTO Catalyzed by NiFe2O4
Authors: Dave Pragnesh N. , Sirach Ruksana , Thakkar Riddhi , Chaturvedi Shalini
Published: January 2022

Nanosize Nickel ferrite (NiF) was synthesized by the co-precipitation methods and its effect as a 5 % by mass additive was studied on the thermal decomposition of micrometer and nanometer size NTO. In the presence of 5 % NiF additive, the thermal decomposition peak temperature of NTO was decreased from 276.36 to 260.18 oC and that of nano NTO was decreased from 261.38 to 258.89 oC (β=10 oC min-1). The kinetics parameters confirms the catalytic activity of NiF for the thermal decomposition of NTO, and nNTO as the parameters such as activation energy (NTO=~25.45 % and nNTO=~45.94 % decrement), and pre-exponential factor (NTO=~21.94 % and nNTO=~43.12 % decrement) were decreased when 5 % NiF additive was added to NTO, and nNTO. The rate of the decomposition process was increased in the presence of 5 % NiF catalyst, indicating the faster thermal decomposition of both NTO, and nNTO in the presence of nickel catalyst.
Scaling Behavior of InAlN/GaN HEMTs on Silicon for RF Applications
Authors: Cui Peng , Zeng Yuping
Published: January 2022

Due to the low cost and the scaling capability of Si substrate, InAlN/GaN high-electron-mobility transistors (HEMTs) on silicon substrate have attracted more and more attentions. In this paper, a high-performance 50-nm-gate-length InAlN/GaN HEMT on Si with a high on/off current (Ion/Ioff) ratio of 7.28 × 106, an average subthreshold swing (SS) of 72 mV/dec, a low drain-induced barrier lowing (DIBL) of 88 mV, an off-state three-terminal breakdown voltage (BVds) of 36 V, a current/power gain cutoff frequency (fT/fmax) of 140/215 GHz, and a Johnson’s figure-of-merit (JFOM) of 5.04 THz∙V is simultaneously demonstrated. The device extrinsic and intrinsic parameters are extracted using equivalent circuit model, which is verified by the good agreement between simulated and measured S-parameter values. Then the scaling behavior of InAlN/GaN HEMTs on Si is predicted using the extracted extrinsic and intrinsic parameters of devices with different gate lengths (Lg). It presents that a fT/fmax of 230/327 GHz can be achieved when Lg­ scales down to 20 nm with the technology developed in the study, and an improved fT/fmax of 320/535 GHz can be achieved on a 20-nm-gate-length InAlN/GaN HEMT with regrown ohmic contact technology and 30% decreased parasitic capacitance. This study confirms the feasibility of further improvement of InAlN/GaN HEMTs on Si for RF applications.
A Reliable Automated Synthesis of 6-[18F]Fluoro-L-DOPA and the Clinical Application on the Imaging of Congenital Hyperinsulinism of Infants
Authors: Zhang Zhengwei , Ge Jingjie , Jing Kai , Chen Yefeng , Guan Yihui , Xie Hexin , Zhu Jianhua
Published: January 2022

6-[18F]fluoro-L-DOPA is a radiotracer widely used in the diagnosis of a range of diseases, including neuro-oncology, endocrinology, and Parkinson’s disease. To meet the fast growing clinical need for this radioactive compound, this study reports an optimized radiosynthsis of this molecule, which proved to be highly reliable and compatible with different types of automated radiosynthesizers. Moreover, with 6-[18F]fluoro-L-DOPA, the PET/CT imaging of a total of 23 patients has been conducted, further demonstrating this radiotracer as a clinically valuable reagent to diagnose congenital hyperinsulinism (CHI) of infancy and, more importantly, localize the exact lesion on pancreas.
Isolation and Identification of major bacteria from three Ethiopian rift valley lakes live and processed fish, and water samples: Implications in sanitary system of fish products
Authors: Dissasa Guta , Lemma Brook , Mamo Hassen
Published: January 2022

Bacterial pathogens are a great threat to fish production. Gram-negative bacteria are among the major bacterial fish pathogens and zoonotic with the potential to infect humans. This cross-sectional study was conducted to isolate and identify major gram-negative bacteria from live and processed fish, and water samples from Lakes Hawassa, Langanoo and Ziway. A total of 674 different types of samples: 630 tissue samples (210 samples for each intestine, Kidney and liver collected from 210 live fish (Oreochromis niloticus, Cyprinus carpio and Clarias gariepinus), 20 processed fish samples from lake Ziway fish processing center and 24 lake water samples were included in the study from each lake. The mean values of pH, temperature, dissolved oxygen and nitrate in all water samples were within the normal range at which most freshwater fish species become non-stressed. Of a total of 674 samples included in the study, the bacteria were isolated from 154 (22.8%) samples with significant difference (P<0.05) observed in some isolates with respect to sample origin. Of these 154 isolates, 103(66.8%) isolates were gram-negative bacteria consisting of 15 species based on morphology and a range of biochemical tests. From live fish samples, Escherichia coli was the dominant species with 15 isolates followed by Edwardsiella tarda (12), Salmonella Paratyphi (10), Salmonella Typhi (9), Shigella dysenteriae(7), Shigella flexneri (7), Klebsiella pneumonia (7), Enterobacter aerogenes (6), Enterobacter cloacae (5), Pseudomonas aeruginosa (5), Vibrio parahemolyticus (5), Aeromonas sobria (4), Citrobacter freundii (4), Citrobacter koseri (4) and Plesiomonas shigelloides(3). Detection of common fecal coliforms (E. coli, K. pneumoniae and E. aerogenes) and Salmonella spp. in processed fish indicates the potential danger of passage of pathogenic bacteria and/or their poisons to humans via infected and/or contaminated fish products. Human infection by pathogenic fish bacteria and food poisoning is possible through contamination of fish product in fish production chain due to inadequate handling, poor hygiene and contact with contaminated water. Therefore, producers, consumers and all other stakeholders need to be cautious during handling, processing and consumption of fish harvested from the study lakes.
Epigenetic upregulation by valproic acid of transferrin receptor 1, and not glucose transporter 1 or CD98hc, at the blood-brain barrier
Authors: Helgudóttir Steinunn Sara , Johnsen Kasper Bendix , Routhe Lisa Juul , Rasmussen Charlotte L.M. , Karamehmedovic Azra , Thomsen Maj Schneider , Moos Torben
Published: January 2022

BackgroundThe objectives of the present study were to investigate whether the expression of transferrin receptor 1 (TfR1), glucose transporter 1 (Glut1), or Cluster of Differentiation 98 Heavy Chain (CD98hc) is epigenetically regulated in brain capillary endothelial cells (BCECs) denoting the blood-brain barrier (BBB).MethodsThe expression of these targets was investigated both in vitro and in vivo following treatment with the histone deacetylase inhibitor (HDACi) valproic acid (VPA). Mice were injected intraperitoneally with VPA followed by analysis of isolated brain capillaries, and the capillary depleted brain samples. Brain tissue, isolated brain capillaries, and cultured primary endothelial cells were analyzed by RT-qPCR, immunolabeling and ELISA for expression of TfR1, Glut1 and CD98hc. We also studied the vascular targeting in VPA-treated mice injected with monoclonal anti-transferrin receptor (Ri7) conjugated with 1.4 nm gold nanoparticles. ResultsValidating the effects of VPA on gene transcription in BCECs, transcriptomic analysis identified 24,371 expressed genes, of which 305 were differentially expressed with 192 upregulated and 113 downregulated genes. In vitro using BCECs co-cultured with glial cells, the mRNA expression of Tfrc was significantly higher after VPA treatment for 6 h with its expression returning to baseline after 24 h. Conversely, the mRNA expression of Glut1 and Cd98hc was unaffected by VPA treatment. In vivo, the TfR1 protein expression in brain capillaries increased significantly after treatment with both 100 mg/kg and 400 mg/kg VPA. Conversely, VPA treatment did not increase GLUT1 or CD98hc. Using ICP-MS-based quantification, the brain uptake of nanogold conjugated anti-TfR1 antibodies was non-significant in spite of increased expression of TfR1. ConclusionsWe report that VPA treatment upregulates TfR1 at the BBB both in vivo and in vitro in isolated primary endothelial cells. In contrast, VPA treatment does not influence the expression of GLUT1 and CD98hc. The increase in the overall TfR1 protein expression however does not increase transport of TfR-targeted monoclonal antibody and indicates that targeted delivery using the transferrin receptor should aim for increased mobilization of already available transferrin receptor molecules to improve trafficking through the BBB.
Effectiveness of Acupuncture as Adjunctive Therapy in Type 2 Diabetic: Study Protocol for a Randomized Controlled Trial
Authors: Cheok Yean Chin , Shariff Zalilah Mohd , Mun Chan Yoke , Yein Lee Ping , Chuan Ng Ooi
Published: January 2022

Background: The incidence of type 2 diabetes mellitus is increasing worldwide. The literature suggests that acupuncture is a possible complementary therapy for type 2 diabetes mellitus. This study aims to determine the effect of acupuncture as an adjunctive therapy on homeostasis model assessment-insulin resistance (HOMA-IR), adiposity and health-related quality of life (HRQOL) in patients with type 2 diabetes mellitus. Method and design: This randomised, double-blind, placebo controlled and parallel design trial will be carried out in three public university teaching hospitals in Malaysia. Eligible type 2 diabetes mellitus subjects will be randomly assigned to receive either acupuncture (n=30) or a placebo (n=30). The intervention is carried out using acupuncture ‘press’ needle on abdomen area (10 sessions of treatment), twice a week over 6 weeks. Both groups will continue with their routine diabetes care. Primary outcome of HOMA-IR and secondary outcomes of body mass index and waist circumference will be measured at the time of recruitment (baseline) and after completion of 10 sessions (week 7) of the treatment. Additionally, secondary outcome of HRQOL will be measured at the time of recruitment (baseline), after completion of 5 sessions (week 3/4) and 10 sessions (week 7) of the treatment. Any adverse event will be recorded at every visit.Discussion: The findings of this study will provide important clinical evidence for the effect of acupuncture as adjunctive therapy on HOMA-IR, adiposity and HRQOL of type 2 diabetes mellitus. Name of the registry: US National Institutes of Health Clinical Trials Registry (ClinicalTrials.gov). Trial registration Number: NCT04829045 Date of registration: 2 April 2021URL of trial registry record: https://clinicaltrials.gov/ct2/show/NCT04829045?cond=Type+2+diabetes%2C+acupuncture&draw=2&rank=1
Atypical Papillary Thyroid Carcinoma with Squamous Metaplasia and RET/NRAS/TERT/PIK3CA Mutations: A Case Report
Authors: Li Zhenyu , Huang Haiping , Guo Dongfang , Feng Yujie , Zhang Jing , Jiang Qingming
Published: January 2022

Background: Papillary thyroid carcinoma (PTC) with squamous metaplasia is a relatively rare and special subtype adenocarcinoma of thyroid which involves multiple genetic changes. We reported a rare case of atypical PTC with squamous metaplasia and RET/NRAS/TERT/PIK3CA mutations which was confirmed after surgical resection pathologically. Case summary: A 2.5×2.5×2 cm3, smooth, hard, clear boundaries and solid nodule on the left thyroid gland was found on relevant physical examination of the patient. And then, the unilateral radical resection of thyroid carcinoma was performed after diagnosing. The tumor cells were squamous metaplasia and arranged in structures with diffuse growth pattern microscopically. At high magnification, stretched nucleus, nuclear grooves and internuclear pseudoinclusions in tumor cells were detected, and the follicular epithelium cells were atypical. Immunohistochemical staining shown strong positiveness of CK19, TTF-1, P40 and Galectin-3, partial positiveness of TG of tumor cells and negativeness of Calcitonin, which could exclude medullary thyroid carcinoma (MTC). Furthermore, first generation sequencing of 18 PTC related genes techniques shown RET, NRAS, TERT and PIK3CA was mutated. Conclusion: Genetic detection is vital to the diagnosis of thyroid adenocarcinoma, especially for the PTC with atypical morphology or rare metaplasia
Preliminary Study to Investigate the Distribution and Effects of Certain Metals After Inhalation of Welding Fumes in Mice
Authors: Csaba Kővágó , Szekeres Barbara , Szűcs-Somlyó Éva , Májlinger Kornél , Jerzsele Ákos , Lehel József
Published: January 2022

The most important welding processes used are the Gas Metal Arc (GMA) welding, the Tungsten Inert Gas (TIG) welding, and the Manual Metal Arc (MMA) welding processes. The goal of our investigation was to monitor the distribution of iron (Fe), manganese (Mn), calcium (Ca) and magnesium (Mg) in the lung, spleen, liver, and kidney of mice after inhalation exposure of different welding methods using different steel base materials. The treatment groups were the following: MMA-mild steel, MMA-molybdenum-manganese (MoMn) alloy, TIG-mild steel, and TIG-stainless steel. The samples were taken 24- and 96 hours after the treatments.Most importantly, it was found that the Mn concentration in the lung’ samples of the MMA-mild steel and the MMA-MoMn groups was increased extremely at both sampling times and in the spleen’ samples also. In the TIG groups, the rise of the Mn concentration was only considerable in the lungs and spleens at 24h, and emerged concentration was found in the liver in 96h samples. Histopathology demonstrated emerged siderin content in the spleens of the treated animals and in siderin filled macrophages in the lungs mostly in all treated groups. Traces of high-level glycogen retention was found in the MMA groups at both sampling times. Similar glycogen retention in TIG-Ms and TIG stainless group’s liver samples and emerged number of vacuoles, especially in the hepatocytes of the TIG-stainless steel 96h group were also found.The mentioned results raise the consequence that there is a considerable difference in the kinetics of the Mn distribution between the MMA- and the TIG-fume treated groups. Hence, the result suggests that manganese has a particle-size dependent toxico-kinetics property. The anomaly of the glycogen metabolism indicates the systemic effect of the welding fumes. Also, the numerous vacuoles mentioned above show a possible liver-specific adverse effect of some components of the TIG-stainless steel welding fumes.
Clinical and Laboratory Profile of Patients With Anaphylaxis to Fire Ant Venom (Solenopsis Sp) Under Specific Subcutaneous Immunotherapy.
Authors: Watanabe Alexandra Sayuri , Ferreira Marcelo Alves , Ventura Anne Karoline Rocha Medrado , Galvão Clóvis Eduardo Santos , Kalil Jorge , Santos Keity Souza , Castro Fabio Fernandes Morato
Published: January 2022

Anaphylaxis to fire ant venoms (Solenopsis sp) is a significant cause of systemic allergic reaction caused by Hymenoptera stings in children. There are only a few reports about the safety and efficacy of specific immunotherapy. We aim evaluate clinical characteristics, IgE and IgG4 specific responses of patients undergoing immunotherapy with a whole-body extract of Solenopsis sp after one year of the maintenance phase. Thirty-three patients were enrolled due to anaphylaxis by fire ant venom (Solenopsis sp) and underwent specific venom immunotherapy. They were assessed at baseline and one year after the beginning of the maintenance phase for skin test; specific IgE and IgG4 antibodies to fire ant venom; tryptase. All patients included presented a severe anaphylactic reaction. Although two patients (6.25%) presented a tryptase level higher than 11.4 ug/ml, systemic mastocytosis was ruled out. There was no relationship between the severity of the reaction with gender, tryptase level, atopy, previous reactions, concentration of the allergen in the skin test or specific IgE level. There was an increase of the specific IgG4/IgE ratio between the two time points. Reactions were local, with only two mild systemic reactions during the build-up phase. Twenty patients had accidental stings during immunotherapy, with 3 presenting only urticaria. This study is unprecedented in evaluating clinical and laboratory data in the fire ant immunotherapy. Our results show that after one year of the maintenance phase, patients did not develop any severe reaction with only a few mild reactions and presented a significant production of specific IgG4.
Uterine Rupture of a Patient With Rudimentary Horn Pregnancy at 26th Gestational Weeks Diagnosed via Magnetic Resonance Imaging: a Case Report
Authors: SARIKAYA SEVCAN , AYBAY MUHSİN NUH
Published: January 2022

Objective: Pregnancy of Rudimentary Horn is a type of ectopic pregnancy, that is recognized almost always during surgical treatment of a rupture of the rudimentary horn. This is an obstetric case diagnosed preoperatively by magnetic resonance imaging (MRI).Case: We report the case of a 19-years-old primigravida patient with rupture of rudimentary horn in 26th gestational week. The patient presented with sudden onset severe abdominal pain in the emergency room. Intraabdominal free fluid is detected. To evaluate the etiology of free fluid and location of the gestational sac, an abdominal magnetic resonance imaging (MRI) scan was planned. The decision of emergent laparotomy is made because of sonographic detection of abdominal excessive -concentrated- free fluid, abdominal tenderness, and 2 points decrease of hemoglobin value in the control hemogram. A rudimentary horn pregnancy and fundal rupture of the rudimentary part of the uterus are diagnosed during the surgical procedure. A baby -live- weighing 450 grams was delivered. The ruptured rudimentary horn and same-sided tuba uterina were surgically removed.Conclusion: Rudimentary horn pregnancy is a rare ectopic pregnancy. Diagnosis is difficult clinically, even with diagnostic imaging modalities. Identifying both cornuas systematically in all patients increases the detection rate. The absence of continuity between the gestational sac’s lumen and the cervical canal on imaging is an important finding. Due to the serious maternal and fetal complications, its detection at an early week may be life-saving.
Clinical Outcomes of a Primary Care Liaison Psychiatry Service for Patients with Complex Persistent Physical Symptoms.
Authors: Schofield Chris , Suarez Lori Edwards , Schofield Nathan
Published: January 2022

Background:
Patients with Persistent Physical Symptoms (PPS) may have chronic physical disorders such as diabetes, COPD, Crohn’s, MS and/or ongoing medically unexplained symptoms, and in either case there can be both physical and [psychiatric aetiologies combined. Such co-morbidity tends to be chronic and hard to manage in any healthcare setting and therefore frequent attendance in healthcare settings is present for these patients. We rolled out a primary care based integrated multidisciplinary liaison psychiatry team to 3 CCG areas in Nottinghamshire in October 2019 to see this patient group and have taken patient reported outcome measures from baseline up to 15 months later as part of normal service evaluation.
Results:
We show here that there are clinically relevant and statistically significant improvements in depressive symptoms (PHQ-9), anxiety symptoms (GAD-7) and physical symptoms (PHQ-15). The improvements in the EQ-5D-5L shows that in a whole health measure there are significant improvements over all and specifically in the anxiety/depression and usual activity domains.
Conclusion:
This is clinically very relevant especially when seen in the context that these results were achieved despite a pandemic affecting the whole population. This model is scalable and integrated providers should look to implement it.
Revisão de pesquisas sobre criatividade e envelhecimento
Authors: Nakano Tatiana de Cássia , Chnaider Janaina , Abreu Isabel Cristina Camelo de
Published: January 2022

O envelhecimento populacional tem propiciado uma mudança na percepção dessa fase, a qual, mais recentemente, passou a ser visualizada como um período que pode ser vivenciado de forma saudável e com qualidade de vida. Nesse contexto a criatividade tem sido reconhecida como uma característica que pode contribuir para um envelhecimento bem-sucedido. Diante disso, o presente estudo buscou analisar a produção científica sobre criatividade e envelhecimento. Após busca nas bases de dados eletrônicas da Capes, Google Acadêmico, Pepsic, Redalyc, Research Gate e Scielo, 45 artigos foram selecionados. A análise dos trabalhos indicou um número pequeno de pesquisas, publicadas especialmente nos últimos anos. A maior parte dos artigos se mostrou de natureza empírica, envolvendo idosos entre participantes de outras idades. Diversas definições foram encontradas como base para os estudos sendo que, para a criatividade, a maior parte se baseia nas definições mais tradicionais (Torrance ou Guilford) enquanto para o envelhecimento, visões positivas e negativas dessa fase se fizeram presentes. Dezesseis diferentes construtos foram investigados nas pesquisas.
Assistência multidisciplinar no acompanhamento de pacientes com pênfigo vulgar
Authors: Da Silva Weslay Rodrigues , Morais Hannah Gil de Farias , Da Costa Lucas Melo , Da Silva Thyago Morais Vicente , Melo Thayanara Silva
Published: January 2022

Este trabalho relata o caso clínico de uma paciente do sexo feminino, 33 anos, com queixa principal de múltiplas ulcerações em mucosa bucal, a qual havia sido previamente diagnosticada com pênfigo vulgar, porém devido aos efeitos adversos da terapia medicamentosa abandonou o tratamento. Foi objetivada a retomada da adesão da paciente ao tratamento, com auxílio multiprofissional, tornando-a corresponsável pela sua melhora clínica e a terapêutica envolveu o uso tópico de pomada de Propionato de Clobetasol 0,05% e a administração sistêmica de Prednisona, atingindo resultados satisfatórios pela ótima evolução clínica.
Regeneração óssea guiada em região estética da maxila: relato de caso
Authors: Michelon Luís Henrique , Pigozzi Lucas Bozzetti , Conde Alexandre , Paulus Marília , Bellan Mariá Cortina , Spineli Felipe , França Guilherme
Published: January 2022

Introdução: A reabilitação com implantes nem sempre é possível devido à falta de quantidade óssea suficiente nos rebordos alveolares, o que acaba sendo um grande problema na recuperação estético-funcional dos pacientes. Diante disso, a Regeneração Óssea Guiada surge como uma ótima técnica para proporcionar aumento de volume ósseo e permitir a instalação de implantes. Objetivo: Descrever um relato de caso de uma cirurgia com a utilização da Regeneração Óssea Guiada com enxerto ósseo heterógeno bovino e auxílio de membrana reabsorvível para posterior colocação de implantes. Materiais e métodos: Para a avaliação do ganho de espessura óssea obtida com essa técnica, procedeu-se a comparação das tomografias computadorizadas de feixe cônico realizadas antes e após 6 meses da cirurgia para reabilitação com quatro implantes. Resultados: Na avaliação tomográfica, evidenciou-se um aumento de espessura óssea na região que variam de 1,5 mm a 4 mm em determinadas áreas, possibilitando a reabilitação com implantes dentários. Conclusão: O enxerto heterógeno bovino utilizado com a técnica de Regeneração Óssea Guiada tem apresentado uma grande capacidade de promover aumento ósseo, além de apresentar grau de reabsorção dentro dos limites para instalação dos implantes. Ademais, este tipo de enxerto traz um maior conforto ao paciente, já que elimina a necessidade de um segundo ato cirúrgico, sendo, portanto, mais uma opção de tratamento em casos de defeitos ósseos horizontais em região anterior de maxila.
Discussão no rastreio de anormalidades renais em portador de apêndice pré-auricular com risco de alteração na organogênese: relato de caso
Authors: Contrucci Bruno Antunes , Pinato Gustavo Rogério , Teles Marina Vanzela Lania , Faria Gabriella Maset da Silva
Published: January 2022

A incidência de anormalidades congênitas do trato urinário associadas a alterações congênitas esporádicas em orelha externa é relatada na literatura, principalmente quando fatores de riscos pré-natais que modificam a embriogênese e organogênese fetal estão presentes. A presença de malformações renais predispõe a aumento da morbimortalidade relacionados a cálculos, infecções urinárias de repetição, sepse e doença renal crônica. Contudo, o rastreio de alterações renais, frente à presença de apêndice pré-auricular, não é consenso na literatura, mesmo quando há fatores que aumentam o risco para seu surgimento. Nesse contexto, a utilização da ultrassonografia abdominal em neonatos com apêndice pré-auricular e que possuem risco aumentado para anormalidades congênitas, assume destaque no diagnóstico precoce de condições que possam a vir, futuramente, causar repercussões clínicas. Assim, os autores buscaram relatar o caso de recém-nascido com malformação renal evidenciado em exame de imagem, o qual foi solicitado devido a apêndice pré-auricular esporádico evidenciado em exame físico associado à diabetes mellitus, hipertensão arterial sistêmica e tabagismo materno.
Integral estimation of activity of entrepreneurial universities: practical aspect
Authors: Bayramova Gulshan
Published: January 2022

Investigated problem. Implementation of the concept of an entrepreneurial university necessitates the formation of a system of activity indicators of educational institutions.

The main scientific results. It is specified that the activity of entrepreneurial universities and educational institutions that try to comply with this concept should be systematically rated. The author analyzes modern approaches to estimation of the activity of entrepreneurial universities. The own viewpoint to this evaluation on the basis of calculation of an integrated indicator is offered. The main stages of the algorithm for estimating the activity are described. An integrated activity indicator for five universities has been calculated. The ranking of educational institutions in accordance with the calculated desegregated measures and the concept of entrepreneurial university has been held. The results of the analysis showed that the studied educational institutions from the United States are more in line with the concept of the entrepreneurial university than European ones.

The area of practical use of the research results. The practical results of the study can be used in the process of estimating the activity of entrepreneurial universities.

Innovative technological product. The scientific approach to the evaluation of the activity of entrepreneurial universities proposed by the author allows to form their rating, to track the position of each educational institution in the dynamics.

Scope of application of the innovative technological product: educational policy at the micro- and macroeconomic levels.
Analysis of tendencies in the functioning of innovative entrepreneurship in the context of technological progress
Authors: Bayramov Eshgin
Published: January 2022

The object of research is innovative entrepreneurship and the main tendencies in its operation in the world.

Investigated problem. The rapid development of innovative entrepreneurship necessitates are the identification and tracking of tendencies in this area.

The main scientific results. The article identifies tendencies in the functioning of entrepreneurship in the context of technological progress. The main subsystems of an innovative enterprise are characterized. The dynamics of frontier technologies market indicators in the context of innovative entrepreneurship is analyzed. It is noted that the transformation of the structure of the global market of advanced technologies is accompanied by increasing disparities in technological and financial development between countries. The inconsistency of national goals with global ones in the field of frontier technologies is pointed out.

The area of practical use of the research results. The results of the study can be used by representatives of the scientific community, authorities to understand global tendencies in innovative entrepreneurship.

Innovative technological product Identified global tendencies in the functioning of innovative entrepreneurship can be part of technological development strategies.

Scope of application of the innovative technological product: policy of regulation of innovation activity.
Effect of pop sorghum on the quality of gluten-free cereal bars
Authors: Aleksandrova Anhelina , Mykolenko Svitlana , Tymchak Dmytro , Aliieva Olha
Published: January 2022

Subject of research: technology for the production of gluten-free cereal bars

Investigated problem: the development of gluten-free bars with an improved recipe composition, enriched with biologically valuable substances, in particular, for people suffering from gluten intolerance.

Main scientific results: Changes in the amino acid composition of sorghum grain due to microwave processing were determined. Formulations of cereal bars were developed using the following ingredients: bananas, dates, coconut flakes, buckwheat flakes, almonds, flax seeds, sorghum pop sorghum, vanilla sugar. The advantages of the biological value of pop sorghum as a promising ingredient in bars were revealed. The influence of the content of pop sorghum in the amount of 4, 6, 8 % in the recipe of cereal bars on their organoleptic and structural-mechanical properties was determined, the nutritional and energy value of the products was established. It was revealed that the introduction of pop sorghum into the composition of the recipe for cereal bars had a significant effect on the maximum load, deformation at maximum load and the coefficient of elasticity of bars. Practical recommendations on the recipe composition of gluten-free cereal bars for pop sorghum and technological modes of their production were developed.

The area of practical use of the research results: food enterprises for the production of snacks and functional products, in particular, food concentrate enterprises, restaurants, craft food production.

Innovative technological product: technology for the production of gluten-free cereal bars with the addition of pop sorghum

Scope of application of an innovative technological product: manufactures engaged in the production of functional food products with high nutritional value in a relatively small volume are an excellent source of energy, dietary fiber, vitamins, macro- and microelements for the human body, in particular, consumers suffer from gluten intolerance.
IMPROVING THE EFFECTIVENESS OF TEACHING STUDENTS A FOREIGN LANGUAGE BASED ON A COMMUNICATIVE METHODOLOGY
Authors:   Tashkent State University of Economics Islam Karimov 49 100066 Tashkent, Uzbekistan., Ziyoda Pulatova
Published: January 2022

In the context of globalization and integration in the country into the international community, the modernization of foreign language teaching in domestic higher education is taking place. The development of international contacts in the country causes a need for specialists of various profiles who are well-versed in foreign languages, but their training does not always give the desired results. Practice has shown that the grammatical translation method used in the last few decades to teach a foreign language to specialists of various profiles, in which the emphasis is on learning rules and translating texts, rather than on communication, has not fully justified itself. Having generally good grammar knowledge, and skills in the field of translation, graduates of non-linguistic universities have difficulties in communication with foreigners in the course of professional activities, and the question about the level of knowledge of foreign language professionals are increasingly responsible read and translate with a dictionary, which actually means no possession and failure to carry out practical communication in a foreign language.
Adoption of Multiplayer Online Battle Arena to Introduce Wayang Character
Authors: Andre Andre
Published: January 2022

Wayang is considered a cultural heritage that has been passed down from generation to generation in Indonesia. However, the younger generation known as digital natives shows a lack of motivation and interest in Wayang. Therefore, it became an important matter to introduce traditional Wayang to younger audiences in engaging formats. This paper proposes an adaptation of the multiplayer online battle arena or known as MOBA. This type of multiplayer game for the mobile market is gaining high popularity among the young generation because it embraces quick-paced, strategic, and intense battle scenes. We called our game "Wayang Tarung" or "Wayang Battle" in English. Furthermore, the game has several features supporting the narrative of wayang characters, such as simple digital motion comics and character briefing between loading screens. A mechanic-dynamic-aesthetic framework (MDA) is used as a guideline to assure the game has engaging gameplay. Since the game runs asymmetrically, the starting condition for both players must be considered. Therefore, we also apply a game balancing strategy to ensure that each Wayang character has weak and strong points. In addition, we were adapting artificial intelligence from the Clash Royale game to dictate autonomous character behavior. The implementation of the Wayang Tarung game has been evaluated using a heuristic testing method adapted from the norman Nielsen heuristic usability. We also conducted a direct observation in a controlled-lab condition and gathered the data using a pre-post test that evaluated player knowledge of Wayang's character. Based on the excellent mark pre-post test result, we believe that adopting the multiplayer game platform in introducing wayang characters to the young generation has enriched prominent to explore further.
REVISIÓN SISTEMÁTICA DE LITERATURA: EVOLUCIÓN DEL ESPACIO ÁULICO EDUCATIVO EN EL CONTEXTO COVID-19
Authors: Luévano Hernández Uriel , Ramírez Ramírez Leticia Nayeli
Published: January 2022

La concepción, el diseño y la construcción del espacio áulico en la educación han experimentado transformaciones a lo largo de la historia; se ha transitado de un espacio tradicional en el que se privilegiaba la funcionalidad, el asoleamiento, la ventilación y la escala, a otro diseño –bajo el contexto COVID-19– en el que se prioriza la flexibilidad, el recálculo de aforos, circulaciones de aire y espacios abiertos. El objetivo de esta investigación es realizar una revisión sistemática de literatura que contemple las investigaciones empíricas sobre la configuración del espacio áulico en la educación, durante el período de 2015 a 2021. Como criterios de inclusión se tuvieron en cuenta los siguientes: artículos que abordan el tema del espacio áulico de aprendizaje; publicaciones entre 2015-2021, y las realizadas en el contexto de Iberoamérica. Se utilizaron las bases de datos de acceso abierto SCIELO, REDALYC y motores de búsqueda como INVENTIO. En total se recopiló una base de datos de 111 artículos de los cuales se filtraron, sustentados en los criterios de inclusión, 18 artículos seleccionados. Los resultados se analizaron con base en la estrategia de análisis de contenido. Se identificaron ocho de las investigaciones de España, tres de México, tres de Colombia, una de Perú, una Ecuador, una de Venezuela y una de Panamá. Se concluye la necesidad de crear espacios áulicos que cumplan con los criterios de aforo limitado y ventilación, para el contexto COVID-19, sin descuidar el empleo de estrategias que promuevan el aprendizaje colaborativo.
ENDÜLÜS MÜSLÜMANLARININ İSPANYA’DAN SÜRGÜNÜNÜN OSMANLI COĞRAFYASI VE EKONOMİSİ ÜZERİNDEKİ ETKİLERİ: KUZEY AFRİKA ÖRNEĞİ
Authors: BAL Faruk
Published: January 2022

İspanyol Yahudilerinin İspanya’dan sürgünü, İstanbul, Selanik ve İzmir gibi Osmanlı topraklarına iskânları ile İmparatorluk coğrafyasındaki etkileri üzerine çok sayıda çalışma yapılmasına rağmen Endülüs Müslümanlarının göçünün sosyal ve ekonomik sonuçlarına temas eden çalışmalar sınırlı sayıda kalmıştır. 1492’de İberya Yarımadası’ndaki son Müslüman şehri Gırnata’nın İspanyol Krallığı’na teslim olmasından itibaren başlayan büyük göç dalgaları, 1609 yılında Yarımada’da kalan Müslümanların tamamının sürgün kararı alınmasıyla birlikte 1609-1614 tarihleri arasında en yoğun dönemini yaşamıştır. Sürgün edilen Müslümanların yerleştiği yerlerin başında Osmanlı hâkimiyetindeki Kuzey Afrika toprakları gelmekteydi. Bu sürgünün hem İspanyollar hem de Osmanlılar için önemli sonuçları olmuştur. Müslümanların sürgünü belirli oranda İspanya’nın ekonomik olarak gerilemesinin nedenleri arasında görülürken, Kuzey Afrika’daki iktisadi dinamizmin de başlıca faktörlerinden biri olarak değerlendirilmektedir. Farklı ürünleri ve üretim tarzlarını Kuzey Afrika’ya taşıyan Endülüslü Müslümanlar bölgenin gelişmesine ciddi oranda katkı sağlamışlardır. Bu makalede Endülüs Müslümanlarının yerleştikleri bölgelerde sosyal ve ekonomik hayata yaptıkları katkılar ve bunların sonuçları tartışılacaktır.