bluuebunny/crossref_metadata_2025 huggingface dataset:
Development of an Objective Methodology for Identifying the Sea-Breeze Circulation and Associated Low-Level Jet in the New York Bight
Authors: McCabe Elizabeth J. , Freedman Jeffrey M.
Published: February 2023

In a midlatitude coastal region such as the New York Bight (NYB), the general thermodynamic structure and dynamics of the sea-breeze circulation is poorly understood. The NYB sea-breeze circulation is often amplified by and coterminous with other regional characteristics and phenomena such as complex coastal topology, a low-level jet (LLJ), and coastal upwelling. While typically considered a summertime phenomenon, the NYB sea-breeze circulation occurs year-round. This study creates a methodology to objectively identify sea-breeze days and their associated LLJs from 2010 to 2020. Filtering parameters include surface-based observations of sea level pressure (SLP) gradient and diurnal tendencies, afternoon wind speed and direction tendencies, air temperature gradient, and the dewpoint depression. LLJs associated with the sea-breeze circulation typically occur within 150–300 m MSL and are identified using a coastal New York State Mesonet (NYSM) profiler site. Along coastal Long Island, there are on average 32 sea-breeze days annually, featuring winds consistently backing to the south and strengthening at or around 1800 UTC (1400 EDT). The NYB LLJ is most frequent in the summer months. Sea-breeze days are classified into two categories: classic and hybrid. A classic sea breeze is driven primarily by both cross-shore pressure and temperature gradients, with light background winds; while a hybrid sea breeze occurs in combination with other larger-scale features, such as frontal systems. Both types of sea breeze are similarly distributed with a maximum frequency during July.
Communicating Economic Evidence About Immigration Changes Attitudes and Policy Preferences
Authors: Allen William L. , Ahlstrom-Vij Kristoffer , Rolfe Heather , Runge Johnny
Published: February 2023

Existing studies demonstrate that threat perceptions matter for immigration attitudes. However, while these perceptions are potentially sensitive to information about immigrants’ impacts, questions remain about whether inserting such information into public debates changes attitudes and policy preferences—especially on polarizing issues like immigration. Moreover, few studies have considered messages featuring the type of nonphotorealistic visual elements that increasingly appear in media. Using a survey experiment fielded in the United Kingdom, we examined whether evidence about European Union immigrants’ modestly positive economic impacts on the United Kingdom—presented either as text, with visualizations, or as an animated film—changed immigration attitudes and policy preferences. Although visual elements did not have an effect over and above text, all the informational treatments moved attitudes and preferences in positive directions, even among Leave voters. Our study brings together research on immigration public opinion and visual media and has implications for policymaking and journalism practice.
Knowing Palmyra: Mandatory production of archaeological knowledge
Authors: Baird J. A. , Kamash Zena , Raja Rubina
Published: February 2023

During the Syrian conflict, ongoing since 2011, Palmyra became notorious for the destruction and looting of its Roman-period remains, giving rise to many narratives of what Palmyra’s future should bring, often without attention to how we have come to know its past. This article explores that past through a key period—the French Mandate—when European archaeologists categorically reshaped the site, culminating in the relocation of the site’s population from mudbrick houses in and around the Temple of Bel to a new, military-built town north of the original. We examine the site immediately prior to that transformation through contemporary archaeological diaries from 1924 to 1928, written by Danish archaeologist Harald Ingholt. Through his diaries, it is possible to reconstruct the complexity of knowledge production at the site, which disrupts the authorized discourses of archaeological discovery with important consequences for how we understand the contribution of local inhabitants to scientific knowledge.
An investigation into carbon structures in iron-loaded charcoal based on the Raman shift and line-shape of the G´-band
Authors: Yamagishi Takayuki , Shibutani Sakae , Yamauchi Shigeru
Published: February 2023

Charcoal samples prepared from wood impregnated with Fe3+ were characterized using Raman spectroscopy to investigate ordered structures consisting of sp2-carbon atoms. The iron-loaded charcoal samples were synthesized at relatively low temperatures (600–850 °C). Most of the Raman peak-tops of G´-bands observed in the charcoal were positioned in the narrow range of 2700–2690 cm−1; however, the G´-band positions of the charcoal synthesized in a carbonization-temperature (CT) range (650–750 °C) varied widely with measurement points and some of them displayed wavenumber values lower than 2690 cm−1. The G´-band positions were also affected by holding time at CT. None of the G´-bands observed in the iron-loaded charcoal exhibited positive asymmetrical line-shapes, unlike graphite. We proposed the ordered structures of sp2-carbon atoms in the iron-loaded charcoal based on the Raman spectral features of the G´-bands.
Impact of rumination on sleep quality among patients with non‑alcoholic fatty liver disease: a moderated mediation model of anxiety symptoms and resilience
Authors: Chang Xiaolin , Guo Chenxi , Zhou Heng , Liu Li
Published: February 2023

Background

Poor sleep raises the risk of non-alcoholic fatty liver disease (NAFLD) and hastens disease progression. It is critical to figure out what factors impact the sleep quality of NAFLD patients. The present study aimed to investigate the role of anxiety symptoms in accounting for the impact of rumination on sleep quality and the moderating role of resilience on the associations of rumination with anxiety symptoms and sleep quality.

Methods

In the cross-sectional study, 285 NAFLD patients completed the Chinese version of the Pittsburgh Sleep Quality Index, the Ruminative Responses Scale, the Generalized Anxiety Disorder 7-item scale, and the 14-item Resilience Scale to measure sleep quality, rumination (including brooding and reflection), anxiety symptoms, and resilience, respectively. The PROCESS macro for SPSS v4.0 procedure was applied to perform moderated mediation analysis.

Results

The roles of anxiety symptoms in accounting for the positive associations of brooding, reflection and rumination with poor sleep quality were revealed. It was found that there was a significant moderating role of resilience on the positive associations of brooding, reflection and rumination with anxiety symptoms, which were gradually reduced as resilience increased. The direct associations between brooding, reflection and rumination and poor sleep quality were not significantly moderated by resilience. Thus, a moderated mediation model involving anxiety symptoms and resilience for explaining the impact of rumination on poor sleep quality was supported among patients with NAFLD.

Conclusions

Rumination (including brooding and reflection) could be positively related to poor sleep quality, and anxiety symptoms had a significant role in accounting for the relationship among patients with NAFLD. Resilience showed a moderating role that could attenuate the positive association between rumination and anxiety symptoms. Interventions aimed at alleviating rumination, reducing anxiety symptoms, and enhancing resilience could improve the sleep quality of NAFLD patients.
The role of health protection teams in reducing health inequities: findings from a qualitative study
Authors: Allison Rosalie , Roberts David J , Briggs Adam , Arora Shona , Anderson Sarah
Published: February 2023

Introduction

The UK Health Security Agency’s (UKHSA) Health Protection Teams (HPTs) provide specialist public health advice and operational support to NHS, local authorities and other agencies in England. The development of a three-year UKHSA Health Equity strategy creates a unique opportunity for HPTs to reduce health inequities within their work.

Aims

This study aimed to understand current health equity activities and structures within HPTs, and to propose future HPT-led health equity activities.

Methods

Between November 2021 - March 2022, HPT staff from the nine UKHSA regions were invited to participate in a semi-structured interview or focus group.

Results

Twenty-seven participants covering all nine UKHSA regions took part in a total of 18 interviews and two focus groups. There was enthusiasm to address health inequity, and many reported this as their motivation for working in public health. All HPTs routinely engaged in health equity work including, variously: liaising with other organisations; advocacy in case and outbreak management meetings; developing regional HPT health equity action plans; and targeting under-served populations in day-to-day work. HPT staff discussed the challenge of splitting their time between reacting to health protection incidents (e.g., COVID as the main priority at the time) and pro-active work (e.g., programmes to reduce risk from external hazards for vulnerable populations). Although COVID had raised awareness of health inequities, knowledge of health equity among the professionally diverse workforce appeared variable. Limited evidence about effective interventions, and lack of clarity about future ways of working with other organisations were also shared as barriers to tackling health inequities.

Conclusion

HPTs welcomed the development of UKHSA’s health equity strategy, and through this study identified opportunities where HPTs can influence, support and lead on tackling health inequities. This includes embedding health equity into HPTs’ acute response activities, stakeholder working, and staff management. This study also identified a need for health equity training for HPTs to improve knowledge and skills, utilising evidence-based approaches to health equity. Finally, we have identified areas where HPTs can lead, for example using brief advice interventions and through developing resources, such as standard operating procedures that focus on vulnerable populations. These findings will support a more integrated approach to addressing health equity through health protection work.
Does mandibular advancement with clear aligners have the same skeletal and dentoalveolar effects as traditional functional appliances?
Authors: Wu Yanqi , Yu Qian , Xia Yunhui , Wang Bo , Chen Siyue , Gu Kaijun , Zhang Bojun , Zhu Min
Published: February 2023

Background

The study aimed to compare the dentoskeletal effects of Vanbeek Activator, Herbst, Twin-Block and Mandibular Advancement with clear aligners in children with skeletal Class II malocclusions.

Methods

A sample with sixty-three patients (37 males, 26 females) was included and divided into untreated control group (C, n = 12), Vanbeek Activator group (V, n = 14), Herbst group (H, n = 11), Twin-Block group (TB, n = 12) and MA group (MA, n = 14). Cephalometric analysis and Johnston Pitchfork analysis were performed to quantify the skeletal and dentoalveolar components in molar relationship and overjet correction. Compare the differences of cephalometric data and Johnston-analysis data.

Results

The treatment changes showed significant differences in SNB, FH-NP, NA-PA, Co-Go, Co-Pog, ANB, lower facial height ratio, U1-PP, U6-PP, L1-MP and U1-L1. All the appliances improved overjet relationships significantly (Vanbeek, Herbst, Twin-Block and MA were 2.77 mm, 5.53 mm, 4.73 mm and 3.66 mm respectively) with significant retraction of maxillary incisors. The lower incisor displacement of group V and MA was negative, while that of group H and TB was positive and there were significant differences. Molar relationships were also improved by 3.45 mm, 6.85 mm, 3.48 mm and 0.92 mm for Vanbeek, Herbst, Twin-Block and MA. Mandible displacement showed a trend of group H > TB > V > MA. The displacement of maxillary molars in group H was greater than that in group C, TB and MA, and that of mandibular ones was greater than that in group C, V and MA, significantly. Herbst, Twin-Block and MA have more significant dentoalveolar effect than Vanbeek, while Vanbeek has more skeletal effect than the others especially in restraining maxillary growth.

Conclusions

Four appliances are all effective in mandibular advancement, modification of class II molar relationship and deep overjet, with unavoidable increase in lower facial ratio. Vanbeek Activator has the most skeletal effects. Vanbeek and MA have a good control of mandibular incisors while more compensatory lower incisors proclination in Herbst and Twin-Block. Herbst has greater maxillary molar distalization. MA allows aligning and leveling meanwhile leading the mandible forward.
Palatal changes after treatment of functional posterior cross-bite using elastodontic appliances: a 3D imaging study using deviation analysis and surface-to-surface matching technique
Authors: Lo Giudice Antonino , Ronsivalle Vincenzo , Conforte Cristina , Marzo Giuseppe , Lucchese Alessandra , Leonardi Rosalia , Isola Gaetano
Published: February 2023

Background

The present study aimed to evaluate the changes in palate dimension and morphology after treatment of functional posterior crossbite (FPXB) with elastodontic devices (EAs).

Methods

The treatment group (TG) consisted of 25 subjects (mean age 7.3 ± 0.9 years) who received treatment with EA for one year. The control group (CG) comprised 14 untreated subjects (mean age 6.8 ± 0.7 years). Inclusion criteria: intra-oral scan registered before (T0) and after treatment (T1), FPXB with a mandibular shift towards the crossbite site of ≥ 2 mm, class I molar relationship. Exclusion criteria: missing teeth, anterior crossbite, temporomandibular disorders, previous orthodontic treatment, carious lesions, mobility of deciduous posterior teeth, craniofacial deformities. Digital models were analyzed to assess the inter-canine (ICW) and inter-molar widths (IMW) and the corresponding emi-lateral measurements (eICW and eIMW) using the median palatine plane as reference. According to a specific 3D imaging technology, the morphology and symmetry of the palate was investigated by analysing the 3D deviation between the two specular models of the palate.

Results

At T0, both groups showed a significantly narrower dimension of eICW and eIMW at the crossbite side compared to the non-crossbite side (p < 0.05). Also, the 3D deviation analysis demonstrates a limited matching percentage of the original/mirrored models in both TG (81.12%) and CG (79.36%), confirming the asymmetry of the palate. The area of mis-matching was located at the alveolar bone level. At T1, subjects in the TG showed a significant increment of ICW and IMW (p < 0.05), a reduction of the differences of eICW and eIMW between both sides (p < 0.05) and an increment of the percentage matching (TG = 92.32%) (p < 0.05), suggesting a significant recovery of the palatal asymmetry. No significant changes were found between T0 and T1 in the CG (p > 0.05).

Conclusions

EAs could be successfully used to correct FPXB in mixed dentition and could restore the harmonious development of the palate in children.
Regeneration of immature incisor using platelet rich fibrin: report of a novel clinical application
Authors: Kumar Jishnu Krishna , Surendranath Padmapriya , Eswaramoorthy Rajalakshmanan
Published: February 2023

Background

Endodontic treatment of young permanent teeth with necrotic pulp presents a clinical challenge for the dentist, and conventional endodontic treatment will result in tooth fracture along with a poor prognosis. Regenerative endodontics is a new protocol that has been advanced in the last decades for managing immature permanent teeth. Rare successful management of immature permanent incisors using platelet-rich fibrin is a technique-sensitive procedure.

Case presentation

A 08 years 04 months old female reported the chief complaint of pain in the upper front tooth region for one week. A blunderbuss canal was identified on radiographic examination, and revascularization using platelet-rich fibrin was planned and adopted. After the treatment, apical closure and root lengthening were noted without complications during subsequent follow-ups. Complete periapical healing with greater than 1.5 cm of dentinal thickness was noted.

Conclusion

Revascularization can be considered a viable treatment option for immature nonvital permanent teeth; with advancements in regenerative medicine and clinical practices, revascularization therapies could be developed as a novel mode of treatment in non-vital and dental traumatic cases.
Unusual gingival actinomycosis post allogeneic hematopoietic stem-cell transplant: case report
Authors: Bruno Julia Stephanie , Miranda-Silva Wanessa , Heidrich Vitor , Gonçalves Marianne de Castro , Novis Yana , Arrais-Rodrigues Celso , Camargo Anamaria Aranha , Fregnani Eduardo Rodrigues
Published: February 2023

Background

Allogeneic hematopoietic stem cell transplant (allo-HSCT) is used to treat several hematological diseases, but immunosuppression during allo-HSCT facilitates opportunistic microbial growth in tissues, such as actinomycosis. An effective diagnosis of opportunistic diseases is essential for correct management of the disease and preservation of the immunosuppressed patient’s life.

Case description

A 57-year-old female patient was diagnosed with extranodal nasal type NK/T cell lymphoma and underwent curative treatment with allo-HSCT. Twenty-one days after the last clinical follow-up, the patient presented a necrotizing lesion in the papilla region between the first and second molars of the second quadrant. Histopathological analysis showed the presence of a bacterial cluster consistent with Actinomyces infection, and a dense lymphoid infiltrate was also observed. Immunohistochemistry for CD20, CD3, and CD56 was performed to exclude the possibility of the recurrence of extranodal NK/T cell lymphoma. Oral microbiota profiling showed a huge increase in the abundance of Actinomyces bacteria in the subgingival region three weeks prior to appearance of the lesion.

Conclusions

Opportunistic infections with an unusual clinical appearance are confounding factors in therapeutic decision-making. We present for the first time a case of actinomycosis in the gingival papilla region following allo-HSCT. We also highlight how microbiota profiling through next-generation sequencing could be used to anticipate bacterial infection diagnosis.
Detection of gender-based violence in primary care in Central Catalonia: a descriptive cross-sectional study
Authors: Muñoz-Sellés Ester , Pujolar-Díaz Georgina , Fuster-Casanovas Aïna , Miró Catalina Queralt
Published: February 2023

Background

Violence against women is a serious public health problem. Primary care could be one of the ideal places for the detection of gender-based violence (GBV), since women come into contact with PC at some point in their lives to look after their sexual and reproductive health. The increase in initiatives promoted by the health authorities regarding GBV offers the possibility of observing its evolution over the last few years.

Methods

A descriptive cross-sectional study of reported cases of GBV in the region of Central Catalonia, during the period from 2017 to 2021, was carried out. All women of legal age, belonging to the specified health region and suffering episodes of GBV, were included. The variables analysed were age, area of residence, health diagnoses related to GBV, whether or not they were pregnant at the time of the attack, and mental health history.

Results

Of the total number of women studied, 1,467 presented some type of diagnosis of GBV, with a total of 3,452 episodes reported. We found an increase in the detection of cases, although it must be noted that there is an underreporting of cases in PC. The prevalence according to the total number of women assigned per year over the period studied was 0.42% in 2017 and 0.48% in 2021. It has also been observed that the average number of episodes per woman increased from 1.03 in 2017 to 1.15 in 2021. During the 5 years analysed, the minimum number of episodes per woman was 1 and the maximum was 10. In reference to the duration of the episodes, the minimum was 1 day, and the maximum was 32 years. The mean age of the women was 42.10 years, the most frequent nationality was Spanish (46.60%), and 54.15% of them lived in rural areas.

Conclusions

Despite the established protocols and procedures, it seems that primary health care is not the most frequent place for its detection. It is necessary to continue working to raise awareness and train professionals, and to ensure coordination among all the parties involved in accompanying women in these processes.

Trial registration

CEIm: 21/278-P.
Socio-demographic trends in malaria knowledge and implications for behaviour change interventions in Zanzibar
Authors: Abbas Faiza , Kigadye Emmanuel , Mohamed Fauzia , Khamis Mwinyi , Mbaraka John , Serbantez Naomi , Al-Mafazy Abdul-Wahid , Monroe April , Kiware Samson
Published: February 2023

Background

Zanzibar is among the few places within East Africa that have documented a significant reduction of malaria morbidity and mortality. Despite tremendous gains over the past decade, malaria transmission still persists in Zanzibar. This study aimed at understanding levels of malaria knowledge to provide recommendations that can be used to reinforce and scale up targeted malaria social and behaviour change interventions.

Methods

A descriptive cross-sectional survey was conducted through an administered questionnaire to 431 households selected randomly. The interviewees were the heads of household or representative adults above 18 years. This study investigated the levels of knowledge about the causes, symptoms, and prevention of malaria in areas with high (> 1.9 per 1000) and low (< 1 per 1000) incidence of local malaria cases. The Principal Component Analysis (PCA) was used to compute the composite variable of each category. Descriptive statistics were calculated to understand variables of interest between low and high transmission areas. Multinomial logistic regression model was used to compare knowledge on malaria based on key variables.

Results

A total of 431 heads of households were interviewed. Respondent age, education level, and wealth status were significantly associated with variations in level of malaria knowledge. Old age was found to be significantly associated with low knowledge of malaria (P < 0.001). The majority of study participants who had secondary and higher education levels had good knowledge of malaria (P < 0.006). Participants characterized as middle-income had good knowledge compared to those characterized as low-income (P < 0.001).

Conclusion

The study identified existing gaps in malaria knowledge in low and high transmission areas. Low levels of malaria knowledge were documented among elderly and populations with lower education and income levels. There is a need to extend mobilization, advocacy, and expand channels of communication to reach all community members. The reported gaps in knowledge are important to consider when designing strategies to engage communities in malaria elimination in Zanzibar. Tailored social and behavioural change interventions aiming to increase malaria knowledge could enhance the uptake of malaria prevention services in the community.
PSAT1 positively regulates the osteogenic lineage differentiation of periodontal ligament stem cells through the ATF4/PSAT1/Akt/GSK3β/β-catenin axis
Authors: Jia Linglu , Li Dongfang , Wang Ya-Nan , Zhang Dongjiao , Xu Xin
Published: February 2023

Background

Periodontal ligament stem cells (PDLSCs) are important seed cells for tissue engineering to realize the regeneration of alveolar bone. Understanding the gene regulatory mechanisms of osteogenic lineage differentiation in PDLSCs will facilitate PDLSC-based bone regeneration. However, these regulatory molecular signals have not been clarified.

Methods

To screen potential regulators of osteogenic differentiation, the gene expression profiles of undifferentiated and osteodifferentiated PDLSCs were compared by microarray and bioinformatics methods, and PSAT1 was speculated to be involved in the gene regulation network of osteogenesis in PDLSCs. Lentiviral vectors were used to overexpress or knock down PSAT1 in PDLSCs, and then the proliferation activity, migration ability, and osteogenic differentiation ability of PDLSCs in vitro were analysed. A rat mandibular defect model was built to analyse the regulatory effects of PSAT1 on PDLSC-mediated bone regeneration in vivo. The regulation of PSAT1 on the Akt/GSK3β/β-catenin signalling axis was analysed using the Akt phosphorylation inhibitor Ly294002 or agonist SC79. The potential sites on the promoter of PSAT1 that could bind to the transcription factor ATF4 were predicted and verified.

Results

The microarray assay showed that the expression levels of 499 genes in PDLSCs were altered significantly after osteogenic induction. Among these genes, the transcription level of PSAT1 in osteodifferentiated PDLSCs was much lower than that in undifferentiated PDLSCs. Overexpressing PSAT1 not only enhanced the proliferation and osteogenic differentiation abilities of PDLSCs in vitro, but also promoted PDLSC-based alveolar bone regeneration in vivo, while knocking down PSAT1 had the opposite effects in PDLSCs. Mechanistic experiments suggested that PSAT1 regulated the osteogenic lineage fate of PDLSCs through the Akt/GSK3β/β-catenin signalling axis. PSAT1 expression in PDLSCs during osteogenic differentiation was controlled by transcription factor ATF4, which is realized by the combination of ATF4 and the PSAT1 promoter.

Conclusion

PSAT1 is a potential important regulator of the osteogenic lineage differentiation of PDLSCs through the ATF4/PSAT1/Akt/GSK3β/β-catenin signalling pathway. PSAT1 could be a candidate gene modification target for enhancing PDLSCs-based bone regeneration.
Overview of microbial profiles in human hepatocellular carcinoma and adjacent nontumor tissues
Authors: He Yuting , Zhang Qiyao , Yu Xiao , Zhang Shuijun , Guo Wenzhi
Published: February 2023

Background

Intratumoral microbial communities have been recently discovered to exist in a variety of cancers and have been found to be intricately involved in tumour progression. Therefore, investigating the profiles and functions of intratumoral microbial distribution in hepatocellular carcinoma (HCC) is imperative.

Methods

To verify the presence of microorganisms in HCC, we performed fluorescence in situ hybridization (FISH) using HCC tissues and conducted MiSeq using 99 HCC and paracancerous tissues to identify the key microorganisms and changes in metabolic pathways affecting HCC progression through a variety of bioinformatics methods.

Results

Microbial diversity was significantly higher in HCC tissues than in adjacent tissues. The abundances of microorganisms such as Enterobacteriaceae, Fusobacterium and Neisseria were significantly increased in HCC tissues, while the abundances of certain antitumour bacteria such as Pseudomonas were decreased. Processes such as fatty acid and lipid synthesis were significantly enhanced in the microbiota in HCC tissues, which may be a key factor through which intratumoral microbes influence tumour progression. There were considerable differences in the microbes and their functions within tumour tissue collected from patients with different clinical features.

Conclusion

We comprehensively evaluated the intratumoral microbial atlas of HCC tissue and preliminarily explored the mechanism of the effects of the microbial community involving changes in lipid metabolism and effects on HCC progression, which lays the foundation for further research in this field.
Elucidation of the mechanisms underlying tumor aggravation by the activation of stress-related neurons in the paraventricular nucleus of the hypothalamus
Authors: Yoshida Sara , Hamada Yusuke , Narita Michiko , Sato Daisuke , Tanaka Kenichi , Mori Tomohisa , Tezuka Hiroyuki , Suda Yukari , Tamura Hideki , Aoki Kazunori , Kuzumaki Naoko , Narita Minoru
Published: February 2023

A growing body of evidence suggests that excess stress could aggravate tumor progression. The paraventricular nucleus (PVN) of the hypothalamus plays an important role in the adaptation to stress because the hypothalamic–pituitary–adrenal (HPA) axis can be activated by inducing the release of corticotropin-releasing hormone (CRH) from the PVN. In this study, we used pharmacogenetic techniques to investigate whether concomitant activation of CRHPVN neurons could directly contribute to tumor progression. Tumor growth was significantly promoted by repeated activation of CRHPVN neurons, which was followed by an increase in the plasma levels of corticosterone. Consistent with these results, chronic administration of glucocorticoids induced tumor progression. Under the concomitant activation of CRHPVN neurons, the number of cytotoxic CD8+ T cells in the tumor microenvironment was dramatically decreased, and the mRNA expression levels of hypoxia inducible factor 1 subunit α (HIF1α), glucocorticoid receptor (GR) and Tsc22d3 were upregulated in inhibitory lymphocytes, tumor-associated macrophages (TAMs) and myeloid-derived suppressor cells (MDSCs). Furthermore, the mRNA levels of various kinds of driver molecules related to tumor progression and tumor metastasis were prominently elevated in cancer cells by concomitant activation of CRHPVN neurons. These findings suggest that repeated activation of the PVN-CRHergic system may aggravate tumor growth through a central–peripheral-associated tumor immune system.
Does tidal volume challenge improve the feasibility of pulse pressure variation in patients mechanically ventilated at low tidal volumes? A systematic review and meta-analysis
Authors: Wang Xiaoying , Liu Shuai , Gao Ju , Zhang Yang , Huang Tianfeng
Published: February 2023

Background

Pulse pressure variation (PPV) has been widely used in hemodynamic assessment. Nevertheless, PPV is limited in low tidal volume ventilation. We conducted this systematic review and meta-analysis to evaluate whether the tidal volume challenge (TVC) could improve the feasibility of PPV in patients ventilated at low tidal volumes.

Methods

PubMed, Embase and Cochrane Library inception to October 2022 were screened for diagnostic researches relevant to the predictability of PPV change after TVC in low tidal volume ventilatory patients. Summary receiving operating characteristic curve (SROC), pooled sensitivity and specificity were calculated. Subgroup analyses were conducted for possible influential factors of TVC.

Results

Ten studies with a total of 429 patients and 457 measurements were included for analysis. The predictive performance of PPV was significantly lower than PPV change after TVC in low tidal volume, with mean area under the receiving operating characteristic curve (AUROC) of 0.69 ± 0.13 versus 0.89 ± 0.10. The SROC of PPV change yielded an area under the curve of 0.96 (95% CI 0.94, 0.97), with overall pooled sensitivity and specificity of 0.92 (95% CI 0.83, 0.96) and 0.88 (95% CI 0.76, 0.94). Mean and median cutoff value of the absolute change of PPV (△PPV) were 2.4% and 2%, and that of the percentage change of PPV (△PPV%) were 25% and 22.5%. SROC of PPV change in ICU group, supine or semi-recumbent position group, lung compliance less than 30 cm H2O group, moderate positive end-expiratory pressure (PEEP) group and measurements devices without transpulmonary thermodilution group yielded 0.95 (95%0.93, 0.97), 0.95 (95% CI 0.92, 0.96), 0.96 (95% CI 0.94, 0.97), 0.95 (95% CI 0.93, 0.97) and 0.94 (95% CI 0.92, 0.96) separately. The lowest AUROCs of PPV change were 0.59 (95% CI 0.31, 0.88) in prone position and 0.73 (95% CI 0.60, 0.84) in patients with spontaneous breathing activity.

Conclusions

TVC is capable to help PPV overcome limitations in low tidal volume ventilation, wherever in ICU or surgery. The accuracy of TVC is not influenced by reduced lung compliance, moderate PEEP and measurement tools, but TVC should be cautious applied in prone position and patients with spontaneous breathing activity.

Trial registration PROSPERO (CRD42022368496). Registered on 30 October 2022.
From fox to fork? Toxocara contamination of spinach grown in the south of England, UK
Authors: Healy Sara R. , Morgan Eric R. , Prada Joaquin M. , Betson Martha
Published: February 2023

Background

Toxocara canis and Toxocara cati are intestinal parasites of dogs, cats and foxes, with infected animals shedding eggs of the parasite in their faeces. If humans accidentally ingest embryonated Toxocara spp. eggs from the environment, severe clinical consequences, including blindness and brain damage, can occur. Previous work has demonstrated the presence of Toxocara spp. eggs on vegetable produce grown in the UK, but only in small-scale community gardens. The aim of this study was to determine whether Toxocara spp. eggs are also present on vegetables grown on commercial farms in the UK, which supply produce to a greater number of people.

Methods

A total of 120 samples (300 g each) of spinach (Spinacia oleracea) were collected across four farms in the south of England, UK. The samples were processed using a sieving approach followed by multiplex quantitative polymerase chain reaction analysis.

Results

Overall, 23.0% of samples were positive for T. canis (28/120; 95% confidence interval 16.7–31.7%) and 1.7% for T. cati (2/120; 95% confidence interval 0.5–5.9%). There was a statistically significant difference in the number of positive samples between farms (P = 0.0064). To our knowledge, this is the first report of the isolation of Toxocara spp. from vegetables grown on commercial farms in the UK.

Conclusions

The results of this study highlight the requirement for the thorough washing of vegetables prior to their consumption, especially those such as spinach which may be eaten without first peeling or cooking, and effective farm biosecurity measures to minimise access to farmland by definitive host species of Toxocara spp.

Graphical Abstract
Infestation of pine (Pinus sylvestris L.) seedlings with the pinewood nematode Bursaphelenchus xylophilus Steiner and Buhrer (Nickle) through wood sawdust
Authors: Arbuzova Elena N. , Kulinich Oleg A. , Chalkin Andrey A. , Kozyreva Natalia I. , Gorbach Vyacheslav V. , Ryss Alexander Yu.
Published: February 2023

Key message

There are various pathways for infesting pine trees with the pinewood nematode (PWN), Bursaphelenchus xylophilus. As a result of the experiment, we determined that sawdust infested with the nematode can pose a phytosanitary risk. Nematodes actively moved from infested sawdust into root or stem damaged pine seedlings.

Context

The spread of PWN, Bursaphelenchus xylophilus in pine plantations and forests is caused by beetles of the genus Monochamus, but the nematode can also be introduced via different coniferous commodities.

Aims

The study objective was to assess the possibility that injured roots and stems of Pinus sylvestris seedlings could be infested with PWN through nematode-infested sawdust.

Methods

Experiments of PWN infestation of pines were conducted in a climatic chamber at a temperature of 26 °C and moisture content of 60–70%. After a month in the climatic chamber, the seedlings were exposed to PWN through infested sawdust.

Results

It was determined that PWN actively penetrated seedlings with injured stems when directly exposed to PWN-infested sawdust (83% of seedlings (p < 0.012)). Similar results were obtained in the trial in which during planting, seedlings with damaged roots were exposed to PWN infested sawdust (50% of seedlings (p < 0.008)). The nematodes could not infest seedlings if the sawdust occurred on the soil surface at a distance of 2.5 cm from the seedling stem.

Conclusion

Our results indicate nematode infestation of pine trees can occur through PWN-infested sawdust.
Existence of the positive solutions for boundary value problems of mixed differential equations involving the Caputo and Riemann–Liouville fractional derivatives
Authors: Liu Yujing , Yan Chenguang , Jiang Weihua
Published: February 2023

We prove the existence of the solutions for the new mixed differential equations, which is characteristic of the right-sided Caputo and the left-sided Riemann–Liouville fractional derivatives. There are four major ingredients. The first is composed of some basic definitions and lemmas. The second is the Green’s function of the new mixed fractional differential equations. We calculate the corresponding Green’s functions as well as their properties. The third, which is the main new ingredient of this paper, is demonstration of the existence of the solutions for fractional equations by the fixed-point theorem in cone expansion and compression of norm type. The fourth, as applications, is the example provided to illustrate our main results.
Lysophospholipids and branched chain amino acids are associated with aging: a metabolomics-based study of Chinese adults
Authors: Pan Yiming , Liu Pan , Li Shijie , Li Bowen , Li Yun , Ma Lina
Published: February 2023

Background

Aging is an inevitable process associated with impairments in multiple organ systems, which increases the risk of comorbidity and disability, and reduces the health-span. Metabolomics is a powerful tool in aging research, which can reflect the characteristics of aging at the level of terminal metabolism, and may contribute to the exploration of aging mechanisms and the formulation of anti-aging strategies.

Methods

To identify possible biomarkers and pathways associated with aging using untargeted metabolomics methods, we performed liquid chromatography–mass spectrometry (LC–MS)-based untargeted metabolomics profiling on serum samples from 32 older adults and 32 sex-matched young controls.

Results

Metabolite profiling could distinguish the two groups. Among the 349 metabolites identified, 80—including lysophospholipids whose levels gradually decline—are possible candidate aging biomarkers. Valine, leucine and isoleucine degradation and biosynthesis were important pathways in aging, with reduced levels of l-isoleucine (r = − 0.30, p = 0.017) and l-leucine (r = − 0.32, p = 0.010) observed in older adults.

Conclusions

We preliminarily revealed the metabolite changes associated with aging in Chinese adults. Decreases in mitochondrial membrane-related lysophospholipids and dysfunction of branched-chain amino acid metabolism were determined to be the characteristics and promising research targets for aging.
Architecture for determining the cleanliness in shared vehicles using an integrated machine vision and indoor air quality-monitoring system
Authors: Jayawickrama Nilusha , Ollé Enric Perarnau , Pirhonen Jesse , Ojala Risto , Kivekäs Klaus , Vepsäläinen Jari , Tammi Kari
Published: February 2023

In an attempt to mitigate emissions and road traffic, a significant interest has been recently noted in expanding the use of shared vehicles to replace private modes of transport. However, one outstanding issue has been the hesitancy of passengers to use shared vehicles due to the substandard levels of interior cleanliness, as a result of leftover items from previous users. The current research focuses on developing a novel prediction model using computer vision capable of detecting various types of trash and valuables from a vehicle interior in a timely manner to enhance ambience and passenger comfort. The interior state is captured by a stationary wide-angled camera unit located above the seating area. The acquired images are preprocessed to remove unwanted areas and subjected to a convolutional neural network (CNN) capable of predicting the type and location of leftover items. The algorithm was validated using data collected from two research vehicles under varying conditions of light and shadow levels. The experiments yielded an accuracy of 89% over distinct classes of leftover items and an accuracy of 91% among the general classes of trash and valuables. The average execution time was 65 s from image acquisition in the vehicle to displaying the results in a remote server. A custom dataset of 1379 raw images was also made publicly available for future development work. Additionally, an indoor air quality (IAQ) unit capable of detecting specific air pollutants inside the vehicle was implemented. Based on the pilots conducted for air quality monitoring within the vehicle cabin, an IAQ index was derived which corresponded to a 6-level scale in which each level was associated with the explicit state of interior odour. Future work will focus on integrating the two systems (item detection and air quality monitoring) explicitly to produce a discrete level of cleanliness. The current dataset will also be expanded by collecting data from real shared vehicles in operation.
Protective effect of methanolic extract of Areca catechu nut on ethanol withdrawal symptoms in mice
Authors: Pandy Vijayapandi , Challa Haritha , Byram Preethi
Published: February 2023

Background

The purpose of the current study was to examine the potential impact of a methanolic extract of Areca catechu nut (MAN) on handling-induced convulsions (HIC), anxiety and anhedonia behaviour of alcohol-withdrawn mice. 30 female Swiss albino mice were divided into 5 groups, each with 6 animals. Group 1 (saline withdrawal) received saline during the 3-day alcohol/saline induction phase, while the other 4 groups (alcohol withdrawal) received 20% v/v ethanol (1.25 ml/100 g body weight, i.p.; 20% v/v ethanol was made from absolute ethanol with 79.9 ml saline + 0.1 ml fomepizole, an alcohol dehydrogenase inhibitor). Day four (test day) involved studying handling-induced convulsions; open field test (OFT), elevated plus maze test (EPM), marble burying test (MBT) for anxiety; 24-h sucrose preference test (SPT) for anhedonia in mice. On the test day, Group I and II (saline withdrawal and alcohol withdrawal) received oral treatments with 1% w/v sodium carboxyl methylcellulose 1 h prior to the behavioural testing. Group III received an injection of diazepam (1 mg/kg, i.p., 30 min prior) and Group IV and V were treated with two different doses of MAN (50 and 100 mg/kg, p.o.) 1 h prior to the behavioural test.

Results

At doses of 50 and 100 mg/kg, p.o., the Areca catechu nut methanolic extract significantly reduced handling convulsions and anxiety, and had an anti-anhedonic effect using various evaluation criteria, such as convulsion score (HIC), no. of central and peripheral line crossings (OFT), % entries and time spent in open arms (EPM), no. of marbles buried (MBT), and sucrose intake ratio (SPT) in alcohol-withdrawn mice.

Conclusion

In mice undergoing alcohol withdrawal, Areca catechu nut extract (MAN) greatly lessens handling-induced convulsions, anxiety and depression symptoms.

Graphical Abstract
Absolute acoustic impedance inversion using convolutional neural networks with transfer learning
Authors: Liu Shaoyong , Ni Wenjun , Fang Wenqian , Fu Lihua
Published: February 2023

Acoustic impedance (AI) is a key parameter frequently used for characterizing reservoirs in the oil and gas industry. The absolute AI can be divided into background and high-wavenumber components, which are related to the background velocity and migrated reflectivity, respectively. The relative AI derived from the migrated images is commonly used, but it is generally limited by the wavenumber range of the migrated image. Owing to the advantage of deep learning methods in determining the relationships among data sets, a learning-based AI inversion method that fuses the background model and the migrated reflectivity is proposed to determine the absolute AI. We first acquire learning labels using a synthetic model, and the initial parameters of a convolutional neural network are obtained by training the mapping from the known background AI and the reflectivity to the labels. We then establish a transfer learning scheme to refine the mapping network parameters using the target data. Finally, the target field data are processed using the refined network. The Marmousi model is used as the label for pretraining, and the synthetic Sigsbee2A model and field data are used for validation. Numerical tests demonstrate the effectiveness of the proposed method for absolute AI fusion, and the transfer learning scheme performs well to constrain the inverted AI that has decent consistency with the background model and reflectivity of the target data.
When the illness speaks
Authors: Greenberg Norman R.
Published: February 2023

The nature of culpability and agency in patients with a variety of psychiatric diagnoses is complex. In this article, a psychiatry resident (specialty trainee in psychiatry) reflects on a clinical encounter in medical school to demonstrate some of the benefits (including the removal of stigma) and dangers (including the threat to patients’ agency) of using the medical model to conceptualise psychiatric illness.
Neural correlates of a mindfulness-based intervention in anorexia nervosa
Authors: Noda Tomomi , Isobe Masanori , Mishima Ryo , Tose Keima , Kawabata Michiko , Aso Toshihiko , Tei Shisei , Noma Shun'ichi , Murai Toshiya
Published: February 2023

We examined the neural underpinnings of the effects of mindfulness on anxiety in anorexia nervosa using functional magnetic resonance imaging in 21 anorexia patients. We used a functional magnetic resonance imaging task designed to induce weight-related anxiety and asked participants to regulate their anxiety either using or not using an acceptance strategy. Our results showed reduced activity in the amygdala, anterior cingulate cortex, putamen, caudate, orbital gyrus, middle frontal gyrus, posterior cingulate cortex and precuneus following a mindfulness-based intervention. The present study provides new insight regarding the neural mechanisms underlying the effect of mindfulness-based intervention in ameliorating anorexia nervosa.