bluuebunny/crossref_metadata_2025 huggingface dataset:
Determinants of Occupational Health and Safety Outcomes Among Sanitation Workers Across Worldwide: A Systematic Review on Cross-Sectional Studies
Authors: Tolera Sina Temesgen , Diriba Wegene , Gutema Gebisa Dirirsa , Kaweti Gudeta
Published: November 2023

As a result of working conditions, a variety of determinants or risk factors lead to the development of occupational health and safety impairments or outcomes such as injuries and musculoskeletal disorders among sanitary personnel, which must be identified in order to anticipate concerns. PubMed, Medline, Embase, and Lilacs databases were used from 2010 to April 2022. Searched strategies used logical words “AND/OR”: Occupational *OR Work AND Injuries OR Musculoskeletal Disorder AND Associated Factors [Socio-demographic *OR Behavioral factors *OR Institution Factors *OR Work pattern] AND Sanitary workers [Waste collectors and emptier *OR Street Sweepers *OR Sewage workers *OR Health facilities Cleaners] AND Cross-Sectional Studies. The databases and other collected data and reports yielded a total of 86 studies and finally 16 studies were included. From total of sanitary workers (5833), 4990 (85.5%) were solid waste collectors, and 618 (10.6%) and 225 (3.9%) were and healthcare cleaners and street sweepers, respectively. Regarding associated factors, Age (OR: 22.57, 7.29-69.88); education (OR: 2.22, 1.22-4.00); and experience (OR: 1.92, 1.11-3.31) were predictors for occurrence of injuries. Smoking cigarettes (OR:2.6, 1.55-4.34); sleeping disturbance (OR: 2.57, 1.48-4.47); eating/smoking/drinking at work (OR: 3.85, 1.34-11.06); and lack of personal protective equipment (OR: 2.62; 1.48-4.63) are the other predictors. On other side, Education (OR: 6.73, 1.92-23.51), age (OR: 7.56, 2.18-26.18), and job experience (OR: 10.79, 3.49-33.38) are socio-demographic variables that impact the development of MSDs. Cigarette smoking (OR: 0.14, 0.03-0.64) and job satisfaction (OR: 11.43, 2.04-64.08) are behavioral factors. While, working longer than 8 h (OR: 3.5, 1.543-8.204) and time pressure (OR: 3.25, 1.08-9.77), working for more than 2 h (OR: 8, 2.25; 28.85) and having a bad back (OR: 15.7, 6.47-38.18) were risk factors for MSDs where all P-value < .05. According to current reviewed evidence, socio-demographic indicators, occupational safety with work pattern features, and behavioral factors all significantly contributed to musculoskeletal disorders; occupational injuries among sanitation workers, which require emphasis from government policy and other initiatives.
Culturological analysis of “The Culture Test” and The 3D Gospel
Authors: Cox Roland Paul
Published: November 2023

Jayson Georges’ The Culture Test has over 40,000 respondents from over 200 countries. The test is based on Georges’ three culture types from his book The 3D Gospel: guilt–innocence, shame–honor, and fear–power. Using the discipline of Culturology (also called hologeistic cross-cultural analysis), The Culture Test is analyzed and compared with Hofstede’s cultural dimensions, Minkov and Kaasa’s objective cultural dimensions, and various societal measures. A three-dimensional plot and culture map show how The Culture Test clusters countries and regions. These comparisons, plot, and map highlight the culturological strengths of The Culture Test and The 3D Gospel—correlated to societal measures related to individualism versus collectivism. They also demonstrate the weaknesses of The Culture Test and The 3D Gospel—which measures only one cultural dimension and regions and countries cluster poorly.
Latinx Pre-Immigration Ideological Preferences and Party Identification in the United States
Authors: Jimeno Rafael A. , Rodriguez Javier M. , Garcia John A. , Echeverria-Estrada Carlos A.
Published: November 2023

Much political behavior research highlights the role of post-immigration experiences in framing Latinx immigrants’ preferences in the United States. We contend that immigrants’ pre-migration political socialization also shapes their current views, political engagement, and ideological orientations. Findings from a unique set of 146 interviews on political party choice before and after migration suggest that Latinx immigrants exhibit consistency of political orientations for party choice across borders. In other words, home-country political and ideological orientations help guide choice of U.S. political party. Pre-immigration variables are thus crucial for a more comprehensive assessment of Latinx immigrant political psychology and behavior.
Relational skill training for patient engagement and the creation of a trauma-informed critical care
Authors: Istanboulian Laura , Master Tasneem , Devine Christine , Hamilton Lorrie
Published: November 2023

Patients and families in critical care have a high likelihood of previous and re-experienced trauma. Unaddressed, physical, and psychological impacts of these traumas can worsen outcomes for patients and families. A trauma-informed care approach has been proposed for critical care; however, training programs do not include relational competencies or de-escalation techniques, risking the re-traumatization of patients and families in critical care and negatively impacting clinicians. This article describes a strategy that can be adopted by critical care teams towards the creation of a trauma-informed critical care unit including the use of a framework for relational training. Principles of relationship management and de-escalation are discussed with the use of a fictional exemplar scenario. Key messages include a call to action for relational training for care teams to enhance skilled relational engagement of patients and families. This article also highlights the foundational importance of policies supporting a trauma-informed approach in critical care.
Intravascular papillary endothelial hyperplasia in the lungs of a wild Korean raccoon dog
Authors: Tangchang Warisraporn , Park Su-Young , Jung Eun-Hye , Lee Dong-Min , Choi Bit-Na , Kwon Hyo-Jung , Son Hwa-Young
Published: November 2023

A male Korean raccoon dog of unknown age was rescued and placed at the Daejeon Wildlife Rescue Center, Korea. Physical examination revealed severe emaciation and dehydration, as well as thick crusts and alopecia over most of the body. During medical care, the animal died and was submitted for postmortem examination. Firm, brown-red lesions of various sizes were observed on the surface of the lungs. In cross-sections of the lungs, pulmonary vessels were thickened and dilated, with white irregular papillary luminal projections. Histologically, pulmonary blood vessels were severely hyperplastic, characterized by thickened dilated walls and fibrous papillary projections covered with a single layer of endothelial cells (ECs). Hyperplastic fibrous connective tissue was confirmed by Masson trichrome staining. The ECs expressed CD31. We diagnosed the lesion as intravascular papillary endothelial hyperplasia, a unique non-neoplastic reactive process that has not been reported previously in pulmonary vessels of canids, equids, or felids, to our knowledge.
State-Sponsored Child Separation as Cultural Genocide: Implications for Children’s Rights and Child Adoption
Authors: Mapp Susan C. , Smith Rotabi-Casares Karen
Published: November 2023

State-sponsored child separation as a method of cultural genocide and parental control has a long history around the world. While some may view this only as a historical occurrence, Russian actions in Ukraine and the previous U.S. policy of child separation at its southern border as a deterrent to seeking asylum are contemporary occurrences. Lessons from other countries in the Americas, Africa, and Asia are presented to illustrate the inherent human rights violations of these actions. The implications of cultural genocide are viewed through the lens of applicable United Nations conventions. Ethical implications are presented with actions to ensure the fulfillment of these human rights.
Valoctocogene Roxaparvovec and Etranacogene Dezaparavovec: Novel Gene Therapies for Hemophilia A and B
Authors: Dougherty John A. , Dougherty Kristiann M.
Published: November 2023

Objective:

To review efficacy and safety data of valoctocogene roxaparvovec (Roctavian) and etranacogene dezaparavovec (Hemgenix), novel gene therapies for the treatment of the life-threatening bleeding disorders hemophilia A and B, respectively.

Data Sources:

A PubMed/Google Scholar search from inception through August 11, 2023 was conducted using the following keywords: gene therapy, hemophilia A, hemophilia B, etranacogene dezaparavovec, valoctocogene roxaparvovec, and bleeding.

Study Selection and Data Extraction:

Data, including phase 1 to 3 clinical trials (non-comparator), were obtained from primary literature and package inserts. These reports evaluated clinical pharmacology, efficacy, safety, adverse events, warnings, and precautions.

Data Synthesis:

Valoctocogene phase 3 study in males (n = 134): 87% had factor VIII (FVIII) levels that at least met criteria for mild hemophilia. Etranacogene phase 3 study in males (n = 54): within 3 weeks of infusion, mean factor IX (FIX) levels had reached 26.8 IU/dL. Both therapies provided clinically and statistically significant decreases in bleeding events and prophylactic factor infusions. Most common adverse event was elevations in liver function tests that were treated with glucocorticoids.

Relevance to Patient Care and Clinical Practice in Comparison with Existing Drugs:

The endogenous production of clotting factors mimics physiological production while decreasing morbidity and mortality related to bleeding events similar to the effects of existing replacement strategies. Gene therapy was also shown to increase patient quality of life.

Conclusion:

Valoctocogene and etranacogene provide another treatment for selected patients with hemophilia. Treatment for the patient with hemophilia (gene therapy vs replacement strategy) must be personalized as new clinical data are published being cognizant of drug affordability.
Serum macrophage migration inhibitory factor levels predict brain atrophy in people with primary progressive multiple sclerosis
Authors: Ladakis Dimitrios C , Reyes-Mantilla Maria I , Gadani Sachin P , Mace Jackson W , Dominguez-Penuela Susana C , Appiah Mayaa J , Smith Matthew D , Bhargava Pavan , Fox Robert J , Saidha Shiv , Calabresi Peter A
Published: November 2023

Background:

Macrophage migration inhibitory factor (MIF) is a cytokine linked to multiple sclerosis (MS) progression that is thought to be inhibited by ibudilast. SPRINT-MS was a phase 2 placebo-controlled trial of ibudilast in progressive multiple sclerosis (PMS).

Objective:

To determine whether baseline MIF levels predict imaging outcomes and assess the effects of ibudilast on serum and cerebrospinal fluid (CSF) MIF levels in people with PMS treated with ibudilast.

Methods:

Participants in the SPRINT-MS trial were treated with either ibudilast or placebo and underwent brain magnetic resonance imaging (MRI) every 24 weeks over a duration of 96 weeks. MIF was measured in serum and CSF.

Results:

MIF levels were compared with imaging outcomes in 223 participants from the SPRINT-MS study. In the primary progressive multiple sclerosis (PPMS) cohort, males had higher serum ( p < 0.001) and CSF ( p = 0.01) MIF levels, as compared with females. Higher baseline serum MIF levels in PPMS were associated with faster brain atrophy (beta = −0.113%, 95% confidence interval (CI): −0.204% to −0.021%; p = 0.016). These findings were not observed in secondary progressive multiple sclerosis (SPMS). Ibudilast did not affect either serum or CSF MIF levels.

Conclusions:

Serum MIF levels were associated with male sex and predicted brain atrophy in PPMS, but not SPMS. Ibudilast did not demonstrate an effect on MIF levels, as compared with placebo, although we cannot exclude a functional effect.
Midterm Results of Drug-Coated Balloon and Bare Metal Stent in the Treatment of TASCII C/D Femoropopliteal Artery Occlusive Disease: A Retrospective Multicenter Study
Authors: Xiao Lei , Gu Shuntong , Chen Yonghui , Zhang Xiujun , Wang Yuqiang , Dai Xiangchen
Published: November 2023

Purpose:

This study aimed to compare midterm efficacy between drug-coated balloon (DCB) and bare metal stent (BMS) in the treatment of TASCII C/D femoropopliteal artery lesions.

Methods:

The clinical data of patients with TASCII C/D femoropopliteal artery disease admitted to 3 vascular surgery centers from January 2018 to January 2021 were retrospectively analyzed. Patients were divided into DCB group and BMS group. The DCB group was further subdivided into DCB alone subgroup and DCB+BMS subgroup. Study primary outcomes were freedom from clinical-driven target lesion reintervention (FCD-TLR) at 24 months postprocedure. Secondary outcomes included ankle brachial index (ABI), Rutherford classification, major amputation rate, and mortality.

Results:

A total of 410 consecutive patients were enrolled. At 24 months follow-up, in DCB group (114 patients) relative to BMS group (296 patients), FCD-TLR (86.00% vs 71.91%, p=0.039), and ABI (0.53±0.11 vs 0.47±0.19, p=0.007) were higher; mean Rutherford class was lower (1.57±0.68 vs 3.31±1.39, p=0.000); and major amputation rate (0.87% vs 4.05%, p>0.05) and all-cause mortality (8.92% vs 6.41%, p>0.05) were statistically similar. In DCB alone, subgroup relative to DCB+BMS subgroup, FCD-TLR (90.00% vs 85.00%, p>0.05), major amputation rate (0 vs 1.08%, p>0.05), and all-cause mortality rate (9.09% vs 8.69%, p>0.05) were statistically similar, while FCD-TLR was higher in the DCB+BMS subgroup (90.00% vs 71.91%, p=0.045).

Conclusion:

At midterm follow-up of patients treated for TASCII C/D femoropopliteal artery disease, use of DCB or of DCB combined with rescue BMS appeared similarly efficacious, and yielded more favorable outcomes than use of BMS.

Clinical Impact

The efficacy of drug-coated balloons relative to that of bare metal stents for the treatment of complex femoropopliteal artery lesions, particularly TASCII grade D lesions, remains uncertain. There is a scarcity of multicenter comparative studies, and in this multicenter retrospective study with up to 24-month follow-up, use of drug-coated balloon alone or combined with rescue bare metal stent appeared similarly efficacious while yielding more favorable outcomes than use of bare metal stent alone in the treatment of TASCII grade C/D femoropopliteal artery lesions. Randomized studies are warranted.
Association between abstraction level and time: Are future and past more abstract than the present?
Authors: Bausenhart Karin M , Ulrich Rolf , Kaup Barbara
Published: November 2023

Construal level theory suggests that objects or events are represented differently depending on their psychological distance from ourselves. Specifically, objects and events should be represented more abstractly the farther they are removed from direct experience through distance in the spatial, temporal, social, or hypotheticality domains. Bar-Anan et al. reported a key finding supporting this assumed association of the various distance dimensions and abstraction level. In their study, participants responded faster in an Implicit Association Task when temporally near and concrete concepts, as well as temporally far and abstract concepts, were mapped to the same rather than different response keys. In this study, we conceptually replicated this basic finding when employing temporal adverbs relating to present versus future time, and nouns referring to concrete versus abstract concepts (Experiment 1). Evidence for such an association, however, was largely absent (and significantly weaker than in Experiment 1) when temporal adverbs relating to the past were employed as instances of the large temporal distance category (Experiment 2). We propose that the uncertainty associated with the future, as opposed to the past, might play an important role in this temporal asymmetry by increasing psychological distance.
Association of circulating CD34+ cells level and prognosis after ischemic stroke
Authors: Mizuno Takafumi , Hoshino Takao , Ishizuka Kentaro , Toi Sono , Takahashi Shuntaro , Wako Sho , Arai Satoko , Kitagawa Kazuo
Published: November 2023

Background:

CD34 is a transmembrane phosphoglycoprotein and a marker of hematopoietic and nonhematopoietic stem/progenitor cells. In experimental studies, CD34+ cells are rich sources of endothelial progenitor cells and can promote neovascularization and endothelial repair. The potential role of CD34+ cells in stroke patients remains unclear.

Aims:

We aimed to assess the prognostic effect of circulating CD34+ cell levels on the risk of vascular events and functional prognosis in stroke patients.

Patients and methods:

In this prospective observational study, patients with ischemic stroke were consecutively enrolled within 1 week of onset and followed up for 1 year. Patients were divided into three groups according to tertiles of the level of circulating CD34+ cells (Tertile 1, <0.51/µL; Tertile 2, 0.51–0.96/µL; and Tertile 3, >0.96/µL). The primary outcome was a composite of major adverse cardiovascular events (MACEs), including nonfatal stroke, nonfatal acute coronary syndrome, major peripheral artery disease, and vascular death. The secondary outcomes included the modified Rankin scale (mRS) scores.

Results:

A total of 524 patients (mean age, 71.3 years; male, 60.1%) were included. High CD34+ cell levels were associated with younger age ( p < 0.001) and low National Institutes of Health Stroke Scale scores at admission ( p = 0.010). No significant differences were found in the risk of MACEs among the three groups (annual rates: 15.0%, 13.4%, and 12.6% in Tertiles 1, 2, and 3, respectively; log-rank p = 0.70). However, there were significant differences in the mRS scores at 3 months (median (interquartile range); 2 (1–4), 1 (1–3), and 1 (0–2) in Tertiles 1, 2, and 3, respectively; p = 0.010) and 1 year (3 (1–4), 2 (1–4), and 1 (0–3); p < 0.001) among these groups. After multivariable adjustments, a higher CD34+ cell level was independently associated with good functional outcomes (mRS score of 0–2) at 3 months (adjusted odds ratio (OR), 1.43; 95% confidence interval (CI), 1.01–2.05) and 1 year (adjusted OR, 1.53; 95% CI, 1.09–2.16).

Conclusion:

Although no correlations were found between circulating CD34+ cell levels and vascular event risk, elevated CD34+ cell levels were associated with favorable functional recovery in stroke patients.

Data access statement:

Data supporting the findings of this study are available from the corresponding author on reasonable request.

Clinical trial registration:

The TWMU Stroke Registry is registered at https://upload.umin.ac.jp as UMIN000031913.
Collective Emotion: A Framework for Experimental Research
Authors: Chung Victor , Grèzes Julie , Pacherie Elisabeth
Published: November 2023

Research on collective emotion spans social sciences, psychology and philosophy. There are detailed case studies and diverse theories of collective emotion. However, experimental evidence regarding the universal characteristics, antecedents and consequences of collective emotion remains sparse. Moreover, current research mainly relies on emotion self-reports, accounting for the subjective experience of collective emotion and ignoring their cognitive and physiological bases. In response to these challenges, we argue for experimental research on collective emotion. We start with an overview of theoretical frameworks to identify a set of three characteristics of collective emotion. Based on research in cognitive and affective sciences, we then examine the corresponding candidate mechanisms. Finally, we highlight outstanding questions, review experimental evidence, and suggest ideas for future experimental research.
Image Timing After COVID-19 Infection in Athletes
Authors: Meester Scott , Balcik Brenden J. , Chill Nicholas , Lockrem Justin , Monseau Aaron J.
Published: November 2023

Background:

Coronavirus disease 2019 (COVID-19) has significantly impacted National Collegiate Athletic Association (NCAA) athletics, with specific concerns for cardiac involvement after infection. Pericardial abnormalities have been seen in up to 39.5% of athletes after COVID-19 infection, while myocardial involvement has been reported at a lower rate of 2.7%. To date, myocardial injury has been seen in 0.6% to 0.7% of athletes when using symptom screening and imaging as clinically indicated, which increases to 2.3% to 3.0% when all athletes with COVID-19 undergo cardiac magnetic resonance (CMR) imaging.

Purpose:

This study will examine whether there exists an ideal time from positive COVID-19 results to obtaining imaging to increase the likelihood of finding abnormalities.

Study Design:

Prospective cohort study

Level of Evidence:

Level 3.

Methods:

NCAA athletes at West Virginia University who were found to be COVID-19 positive on routine screening were required to undergo echocardiography (ECG) and CMR. These data were reviewed by cardiology and determined to be normal or abnormal. Statistical analysis with logistic regression and descriptive statistics was performed to evaluate whether a time existed where abnormalities on imaging were most likely to be found.

Results:

A total of 41 athletes were included in this study. ECG was performed earlier on average than CMR imaging, at 18.2 days versus 27.5 days. No significant difference was found in timing from COVID-19 infection diagnosis and abnormalities seen on imaging for either ECG or CMR imaging.

Conclusion:

The risk of cardiac involvement in athletes in the setting of COVID-19 has already been documented. This study suggests that imaging timing is independent of cardiac involvement with no correlation to specific time periods where more abnormalities may be found. However, CMR imaging showing changes at day 54 after infection suggests cardiac findings can be seen months after imaging.

Clinical Relevance:

Cardiac imaging for athletes after contracting COVID-19 does not show a significant relationship to time of imaging. However, given the cardiac involvement seen months after diagnosis, further examination of prolonged cardiac effects must be carried out.
The emerging role of digital health in the management of asthma
Authors: Kaplan Alan , Boivin Michael , Bouchard Jacques , Kim James , Hayes Sean , Licskai Christopher
Published: November 2023

The most common reasons seen for lack of asthma control include misconceptions about disease control, low controller treatment adherence, poor inhaler technique, and the resulting underuse of controllers and overuse of short-acting beta2 agonists (SABAs). Narrowing these care gaps may be achieved through well-designed patient education that considers the patient’s motivation, beliefs, and capabilities regarding their asthma and its management and empowers the patient to become an active participant in treatment decisions. Digital health technologies (DHTs) and digital therapeutic (DT) devices provide new opportunities to monitor treatment behaviors, improve communication between healthcare providers and patients, and generate data that inform educational interactions. DHT and DT have been proven effective in enhancing patient self-management in other chronic conditions, particularly diabetes. Accelerated integration of DHT and DT into the management of asthma patients is facilitated by the use of digital inhalers that employ sensor technology (“smart” inhalers). These devices efficiently provide real-time feedback on controller adherence, SABA use, and inhaler technique that have the strong potential to optimize asthma control.
Psychosocial interventions to improve adherence in depressed and anxious older adults prescribed antidepressant pharmacotherapy: a scoping review
Authors: Stahl Sarah T. , Kincman Joelle , Karp Jordan F. , Anne Gebara Marie
Published: November 2023

Medication nonadherence in depressed and anxious older adults is prevalent and associated with non-response to antidepressant pharmacotherapy. Evidence-based options to improve medication adherence are limited in this population. To review the state of the literature on the types and efficacy of psychosocial interventions for improving antidepressant pharmacotherapy adherence in depressed and anxious older adults. We conducted a scoping review according to PRISMA-ScR guidelines. PubMed/Medline and article references starting in 1980 up to 28 February 2023 were reviewed. Of the 710 records screened, 4 psychosocial interventions were included in the review. All studies included depressed older adults, and none included anxious older adults. Samples included racial and ethnic minorities and were primarily women. The psychosocial interventions consisted mainly of psychoeducation with usual care as the control comparison. Measures of antidepressant adherence included self-reported adherence or pill counting. Three of the four randomized controlled trials improved medication adherence rates and reduced depression symptom burden. Effective interventions exist for improving antidepressant medication adherence in depressed older adults. Improved adherence can reduce depression symptom burden. The lack of interventions for anxious older adults highlights the need to develop and deliver interventions for anxious older adults prescribed antidepressant pharmacotherapy.
Should I Leave My Country? Higher Education Value Shaping Students’ Satisfaction and Brain Drain Intentions in Western Balkans
Authors: Petreska Nina , Prodanova Jana , Kocarev Ljupco
Published: November 2023

Following the relevant literature on higher education services, the objective of this study explores how the higher education perceived value outlines students’ satisfaction with the service and their migration intentions. Data from1,150 university students in Macedonia was collected and analyzed using Partial Least Squares Structural Equation Modeling. The results showed that functional, emotional, conditional and image value perceived about the degree are significant incitements of overall satisfaction, while social influence by reference groups minimizes satisfaction. In turn, students’ satisfaction decreases their intentions to leave the country. Moreover, the year of study acts as a moderator by delivering higher satisfaction, hence reducing brain drain intentions, for students in higher courses. The conclusions of this research emphasize the need for universities to increase appreciation of the higher education system’s quality, efficiency, and reputation. Since the beginning of their studies, students should be able to recognize and experience their education as beneficial for their professional future in the country, reducing in this way one of the major concerns in Western Balkans, the brain drain intentions.
From Game Elements to Active Learning Intentions: Exploring the Driving Factors in Digital Learning Platforms
Authors: Cui Zhuoyao , Yang Haochen
Published: November 2023

This study investigates the influence of game elements on active learning intentions within digital learning platforms. Drawing from a situational perspective, we developed a model and validated it using data from 492 respondents collected via questionnaires. Our findings suggest that while social elements enhance active learning intentions, both achievement and immersive elements tend to weaken them. Notably, the spirituality of the learning place serves as a mediator in the relationship between game elements and active learning intentions. Furthermore, we emphasize the moderating effects of different game behavior patterns. This research enriches our comprehension of game elements’ role in shaping active learning intentions on digital platforms, offering valuable insights for educators and platform developers.
Management of Traumatic Arteriovenous Fistula as a Complication of Minimally Invasive Endoscopic Maxillofacial Surgery: A Case Report
Authors: Burris Briana J. , Regenhardt Robert W. , Rabinov James D. , McCain Joseph P.
Published: November 2023

A traumatic arteriovenous fistula (AVF) is a type of acquired vascular malformation, caused by blunt or penetrating trauma. Traumatic AVFs result in a pathologic connection between arterial and venous vasculature, bypassing the corresponding capillaries. When an intra-operative insult to vasculature within a surgical site is implicated in the subsequent formation of a traumatic AVF, the suspected etiology is iatrogenic. Although the most common etiology of traumatic AVFs of the entire body is iatrogenic trauma, intraoperative vascular insults (ie, ligation and cauterization) are routinely delivered within maxillofacial surgical sites, without formation of an acquired vascular malformation. A traumatic AVF in the pre-auricular region is exceedingly rare and has infrequently been described as a complication following minimally invasive temporomandibular joint (TMJ) surgery, managed with endovascular surgery. We describe a rare case of bilateral TMJ arthroscopies complicated by the formation of a pulsatile, traumatic AVF between the right superficial temporal artery and right retromandibular vein, treated with minimally invasive endovascular embolization by the interventional radiology team. This case report highlights the diagnosis of a traumatic AVF in the pre-auricular region and multi-specialty management of an early complication of minimally invasive maxillofacial surgery.
How I treat sickle cell disease in pregnancy
Authors: James Andra H. , Strouse John Joseph
Published: November 2023

Fifty years ago, people with sickle cell disease (SCD) were discouraged from becoming pregnant, but now, most should be supported if they choose to pursue a pregnancy. They and their providers, however, should be aware of the physiological changes of pregnancy that aggravate SCD and pregnancy’s unique maternal and fetal challenges. Maternal problems can arise from chronic underlying organ dysfunction such as renal disease or pulmonary hypertension; from acute complications of SCD such as acute anemia, vaso-occlusive crises, and acute chest syndrome; and/or from pregnancy-related complications such as preeclampsia, sepsis, severe anemia, thromboembolism, and the need for cesarean delivery. Fetal problems include alloimmunization, opioid exposure, fetal growth restriction, preterm delivery, and stillbirth. Before and during pregnancy, in addition to the assessment and care that every pregnant patient should receive, patients with SCD should be evaluated and treated by a multidisciplinary team with respect to their unique maternal and fetal issues.
SARS-CoV-2 helicase might interfere with cellular nonsense-mediated RNA decay: insights from a bioinformatics study
Authors: Akbari Behnia , Ahmadi Ehsan , Zabihi Mohammad Reza , Zamir Mina Roshan , Shaker Mina Sadeghi , Noorbakhsh Farshid
Published: November 2023

Background

Viruses employ diverse strategies to interfere with host defense mechanisms, including the production of proteins that mimic or resemble host proteins. This study aimed to analyze the similarities between SARS-CoV-2 and human proteins, investigate their impact on virus-host interactions, and elucidate underlying mechanisms.

Results

Comparing the proteins of SARS-CoV-2 with human and mammalian proteins revealed sequence and structural similarities between viral helicase with human UPF1. The latter is a protein that is involved in nonsense-mediated RNA decay (NMD), an mRNA surveillance pathway which also acts as a cellular defense mechanism against viruses. Protein sequence similarities were also observed between viral nsp3 and human Poly ADP-ribose polymerase (PARP) family of proteins. Gene set enrichment analysis on transcriptomic data derived from SARS-CoV-2 positive samples illustrated the enrichment of genes belonging to the NMD pathway compared with control samples. Moreover, comparing transcriptomic data from SARS-CoV-2-infected samples with transcriptomic data derived from UPF1 knockdown cells demonstrated a significant overlap between datasets.

Conclusions

These findings suggest that helicase/UPF1 sequence and structural similarity might have the ability to interfere with the NMD pathway with pathogenic and immunological implications.
Genome-wide characterization of regulator of chromosome condensation 1 (RCC1) gene family in Artemisia annua L. revealed a conservation evolutionary pattern
Authors: Chen Jieting , Wu Wenguang , Ding Xiaoxia , Zhang Danchun , Dai Chunyan , Pan Hengyu , Shi Peiqi , Wu Chanjuan , Zhang Jun , Zhao Jianmin , Liao Baosheng , Qiu Xiaohui , Huang Zhihai
Published: November 2023

Background

Artemisia annua is the major source for artemisinin production. The artemisinin content in A. annua is affected by different types of light especially the UV light. UVR8, a member of RCC1 gene family was found to be the UV-B receptor in plants. The gene structures, evolutionary history and expression profile of UVR8 or RCC1 genes remain undiscovered in A. annua.

Results

Twenty-two RCC1 genes (AaRCC1) were identified in each haplotype genome of two diploid strains of A. annua, LQ-9 and HAN1. Varied gene structures and sequences among paralogs were observed. The divergence of most RCC1 genes occurred at 46.7 – 51 MYA which overlapped with species divergence of core Asteraceae during the Eocene, while no recent novel RCC1 members were found in A. annua genome. The number of RCC1 genes remained stable among eudicots and RCC1 genes underwent purifying selection. The expression profile of AaRCC1 is analogous to that of Arabidopsis thaliana (AtRCC1) when responding to environmental stress.

Conclusions

This study provided a comprehensive characterization of the AaRCC1 gene family and suggested that RCC1 genes were conserved in gene number, structures, constitution of amino acids and expression profiles among eudicots.
Towards unlocking the biocontrol potential of Pichia kudriavzevii for plant fungal diseases: in vitro and in vivo assessments with candidate secreted protein prediction
Authors: Elkhairy Bassma Mahmoud , Salama Nabil Mohamed , Desouki Abdalrahman Mohammad , Abdelrazek Ashraf Bakry , Soliman Khaled Abdelaziz , Ibrahim Samir Abdelaziz , Khalil Hala Badr
Published: November 2023

Background

Plant fungal pathogens cause substantial economic losses through crop yield reduction and post-harvest storage losses. The utilization of biocontrol agents presents a sustainable strategy to manage plant diseases, reducing the reliance on hazardous chemical. Recently, Pichia kudriavzevii has emerged as a promising biocontrol agent because of its capacity to inhibit fungal growth, offering a potential solution for plant disease management.

Results

Two novel Pichia kudriavzevii strains, Pk_EgyACGEB_O1 and Pk_EgyACGEB_O2, were isolated from olive brine samples. The microscopic characterization of the strains revealed similar structures. However, there were noticeable differences in their visual morphology. Based on their internal transcribed spacer (ITS) DNA sequences, Pk_EgyACGEB_O1 and Pk_EgyACGEB_O2 strains assigned by GenBank IDs MZ507552.1 and MZ507554.1 shared high sequence similarity (~ 99.8% and 99.5%) with P. kudriavzevii, respectively. Both strains were evaluated in vitro against plant pathogenic fungi. The strains revealed the ability to consistently inhibit fungal growth, with Pk_EgyACGEB_O2 showing higher effectiveness. In addition, both P. kudriavzevii strains effectively controlled grey mold disease caused by B. cinerea in golden delicious apples, suggesting their potential as sustainable and eco-friendly biocontrol agents for post-harvest diseases. Based on a comprehensive bioinformatics pipeline, candidate-secreted proteins responsible for the potent antifungal activity of P. kudriavzevii were identified. A total of 59 proteins were identified as common among the P. kudriavzevii CBS573, SD108, and SD129 strains. Approximately 23% of the secreted proteins in the P. kudriavzevii predicted secretome are hydrolases with various activities, including proteases, lipases, glycosidases, phosphatases, esterases, carboxypeptidases, or peptidases. In addition, a set of cell-wall-related proteins was identified, which might enhance the biocontrol activity of P. kudriavzevii by preserving the structure and integrity of the cell wall. A papain inhibitor was also identified and could potentially offer a supplementary defense against plant pathogens.

Conclusion

Our results revealed the biocontrol capabilities of P. kudriavzevii against plant pathogenic fungi. The research focused on screening novel strains for their ability to inhibit the growth of common pathogens, both in vitro and in vivo. This study shed light on how P. kudriavzevii interacts with fungal pathogens. The findings can help develop effective strategies for managing plant diseases.
The neutrophil percentage-to-albumin ratio is associated with all-cause mortality in patients with chronic heart failure
Authors: Wang Xin , Zhang Yuan , Wang Yuxing , Liu Jia , Xu Xiaorong , Liu Jiamei , Chen Mulei , Shi Linying
Published: November 2023

Background

In this study, we evaluated the predictive utility of neutrophil percentage-to-albumin ratio (NPAR) for all-cause mortality in patients with chronic heart failure (CHF).

Methods

Patients diagnosed as CHF enrolled in this retrospective cohort study were from Beijing Chaoyang Hospital, capital medical university. Admission NPAR was calculated as neutrophil percentage divided by serum albumin. The endpoints of this study were defined as 90-day, 1-year and 2-year all-cause mortality. Multivariable Cox proportional hazard regression model was performed to confirm the association between NPAR and all-cause mortality. Receiver operating characteristics (ROC) curves were used to evaluate the ability for NPAR to predict all-cause mortality.

Results

The 90-day (P = 0.009), 1-year (P < 0.001) and 2-year (P < 0.001) all-cause mortality in 622 patients with CHF were increased as admission NPAR increased. Multivariable Cox regression analysis found the higher NPAR value was still independently associated with increased risk of 90-day (Group III versus Group I: HR, 95% CI: 2.21, 1.01–4.86, P trend = 0.038), 1-year (Group III versus Group I: HR, 95% CI:2.13, 1.30–3.49, P trend = 0.003), and 2-year all-cause mortality (Group III versus Group I: HR, 95% CI:2.06, 1.37–3.09, P trend = 0.001), after adjustments for several confounders. ROC curves revealed that NPAR had a better ability to predict all-cause mortality in patients with CHF, than either albumin or the neutrophil percentage alone.

Conclusions

NPAR was independently correlated with 90-day, 1-year, and 2-year all-cause mortality in patients with CHF.
Screening UFMylation-associated genes in heart tissues of Ufm1-transgenic mice
Authors: Jiajia Hu , Ziyao Yang , Jiaqi Zheng , Yanli Chen , Xiaotao Zhao , Ming Su
Published: November 2023

UFMylation is a ubiquitination-like modification that is related to endoplasmic reticulum stress and unfolded protein response. A recent study reported that Ufl1, a key enzyme of UFMylation, protects against heart failure, indicating that UFMylation may be associated with heart function regulation. In the present study, we initially constructed a Flag-6×His-tagged Ufm1ΔSC transgenic (Tg-Ufm1) mouse model that enables UFMylation studies in vivo. Tg-Ufm1 mice showed significant activation of UFMylation in hearts. By using this model, we identified 38 potential Ufm1-binding proteins in heart tissues through LC‒MS/MS methods. We found that these proteins were associated with mitochondria, metabolism and chaperone binding. By using transcriptomic screening, we identified Tnfaip2 as a novel UFMylation-associated gene. Overexpression of Ufm1 significantly upregulated the protein expression of Tnfaip2, whereas isoproterenol treatment decreased Tnfaip2 expression in Tg-Ufm1 mice. These data may provide novel clues for UFMylation in cardiac hypertrophy.
Prevalence and antibiotic resistance pattern of bacteria from sepsis suspected neonates at St. Paul’s Hospital Millennium Medical College, Addis Ababa, Ethiopia
Authors: Sherif Merema , Abera Dessie , Desta Kassu
Published: November 2023

Background

Neonatal sepsis is the major cause of neonatal mortality and morbidity, especially in low and middle-income countries. Continuous monitoring of pathogens and their antibiotic resistance pattern is crucial for managing neonatal sepsis. This study aimed to determine neonatal sepsis due to bacteria, antibiotic resistance patterns, associated risk factors and patient outcomes at St. Paul’s Hospital Millennium Medical College.

Method

An institutional-based cross-sectional study was conducted on 400 neonates suspected of sepsis at St. Paul’s Hospital Millennium Medical College from March 2020 to July 2020. A questionnaire was used to collect socio-demographic information, clinical parameters and potential risk factors from study participants. About 2ml of blood was drawn aseptically and inoculated into Tryptone Soya Broth at the patient’s bedside. Bacterial identification was performed by using standard microbiological techniques. The disk diffusion method was used to determine the antibiotic susceptibility patterns of each isolated bacteria. Data entry and analysis were done using Statistical Package for Social Sciences (SPSS) version 20 software. Bivariate and multivariable logistic regressions were used to assess associated risk factors of neonatal sepsis. A p-value less than 0.05 was considered statically significant with a 95% confidence interval.

Results

The overall prevalence of neonatal septicemia was 21% (84/400). Of these, 67 (79.8%) and 17 (20.2%) were gram-negative and gram-positive bacteria, respectively. Klebsiella spp, 37 (44%), E. coli 19 (21.6%) and Coagulase negative Staphylococci 13 (15.47%) were the leading cause of neonatal sepsis. Ciprofloxacin and amikacin were the most effective antibiotics for gram-negative and gram-positive bacteria. Multidrug resistance was observed in 84% of the bacterial isolates. Low birth weight and preterm were associated with neonatal septicemia (AOR = 49.90, 95% CI = 15.14-123.081, P = 0.002) and (AOR = 18.20, 95% CI = 6.835–27.541, P = 0.004) respectively.

Conclusion

Klebsiella spp and E. coli were frequently isolated bacteria in our study. The proportion of multidrug-resistance was significantly high. Most isolated bacteria were resistant to ampicillin, ceftazidime, cefotaxime and gentamycin, which indicates the necessity of continuous evaluation of antibiotic resistance rate.
Cannulation via the external jugular vein——An alternative to conventional peripherally inserted central catheterisation for paediatric patients
Authors: Zhang Ping , Jia Miao , Li Wan-yuan , Li Juan , Niu Jin-lei , Ding Hong , Zhou Wang-mei
Published: November 2023

Purpose

This study aimed to describe a peripherally inserted central catheterisation (PICC) for paediatric patients with inaccessible access and a high risk of general anaesthesia (GA). Methods: This was a retrospective observational study involving all paediatric inpatients who performed the PICC via an EJV approach without GA between September 2014 and September 2021 in a provincial key clinical speciality. Results: A total of 290 EJV line placement attempts were performed, and 29 were excluded due to missing placement results, resulting in a sample size of 261. The anatomical localisation, punctures, and catheterisation success rates for this practice were 100%, 100%, and 90.04%, respectively. The placement success rate in children younger than one year was 93.75% (45/48). The median line duration of use was 19 days, with a median length of catheter insertion of 13 cm. The most common complications were catheter malposition (n = 20) and dislodgement (n = 7). Conclusion: The PICC via an EJV approach without GA is a feasible and safe practice with acceptable success and complication rates, and low costs. It might be an attractive alternative for obtaining central vascular access for paediatric patients.
Novel mutation in ELN gene causes cardiac abnormalities and inguinal hernia: case report
Authors: Zhang Hua-yong , Xiao Min , Zhang Yong
Published: November 2023

Background

Elastin-driven genetic diseases are a group of complex diseases driven by elastin protein insufficiency and dominant-negative production of aberrant protein, including supravalvular aortic stenosis (SVAS) and autosomal dominant cutis laxa. Here, a Chinese boy with a novel nonsense mutation in the ELN gene is reported.

Case presentation

We report a 1-year-old boy who presented with exercise intolerance, weight growth restriction with age, a 1-year history of heart murmur, and inguinal hernia. Gene sequencing revealed a novel nonsense mutation in the ELN gene (c.757 C > T (p.Gln253Ter), NM_000501.4). Due to severe branch pulmonary artery stenosis, the reconstruction of the branch pulmonary artery with autologous pericardium was performed. The inguinal hernia repair was performed 3 months postoperatively. After six months of outpatient follow-up, the child recovered well, gained weight with age, and had no special clinical symptoms.

Conclusion

We identified a de novo nonsense mutation in the ELN gene leading to mild SVAS and severe branch pulmonary artery stenosis. A new phenotype of inguinal hernia was also needed to be considered for possible association with the ELN gene. Still, further confirmation will be necessary.
Binge drinking and condom negotiation behaviours among adolescent girls and young women living in Cape Town, South Africa: sexual control and perceived personal power
Authors: Singer Suzanne E. , Wechsberg Wendee M. , Kline Tracy , Browne Felicia A. , Howard Brittni N. , Carney Tara , Myers Bronwyn , Bonner Courtney Peasant , Chin-Quee Dawn
Published: November 2023

Background

Condom use among young people in South Africa has declined in recent years and adolescent girls and young women continue to bear the highest incidence of HIV in the country. Young women who have dropped out of school may be more at risk because of traditional gender norms that create substantial power imbalances and a lack of power to negotiate condom use with their male partners, especially when using alcohol and other drugs.

Methods

This study presents an analysis of baseline data provided by 500 adolescent girls and young women (AGYW) from Cape Town communities between November 2016 and November 2018 who were reached for a cluster-randomised trial conducted to assess the efficacy of an evidence-based, young woman-focused intervention seeking to reduce HIV risk and substance use behaviours. The analysis focuses on associations between binge drinking, condom use, and sexual negotiation, including impaired sex (any substance use at last sex).

Results

AGYW who reported frequent condom negotiation with their partners were 8.92 times (95% CI: [4.36, 18.24]) as likely to use a condom when alcohol or other drugs were not used at last sex and 5.50 times (95% CI: [2.06, 14.72]) as likely when alcohol or other drugs were used at last sex (p < 0.05). AGYW who reported frequent binge drinking in the past month (n = 177) had significantly reduced odds of condom use at last sex, irrespective of whether the sex was impaired (OR 0.60, 95% CI: [0.49, 0.73]) or not impaired (OR 0.69, 95% CI: [0.60, 0.81]).

Discussion

The findings highlight the need for interventions that reach AGYW in South Africa by specifically aiming to educate AGYW about the effect of binge drinking on negotiating power in their relationships, thus providing them with the knowledge and skills to increase agency regarding condom use.

Trial registration

ClinicalTrials.gov Identifier: NCT02974998 (recruitment completed). 29/11/2016.