bluuebunny/crossref_metadata_2025 huggingface dataset:
Phenotypic and molecular analysis of insecticide resistance in Aedes albopictus populations from Greece
Authors: Balaska Sofia , Fotakis Emmanouil Alexandros , Kioulos Ilias , Grigoraki Linda , Mpellou Spyridoula , Chaskopoulou Alexandra , Vontas John
Published: April 2020

Background: Aedes albopictus has a well-established presence in southern European countries, associated with recent disease outbreaks (e.g. Chikungunya). Development of insecticide resistance in the vector is a major concern as it’s control mainly relies on the use of biocides. Data on the specie’s resistance status is essential for efficient and sustainable control. Methods: We investigated the insecticide resistance status of several Ae. albopictus populations from Greece. Bioassays were performed against diflubenzuron (DFB), B. thuringiensis var. israelensis (Bti), deltamethrin and malathion. Molecular analysis of known insecticide resistance loci was performed, i.e. voltage-gated sodium channel (VGSC) mutations associated with pyrethroid resistance; presence and frequency of carboxylesterases 3 (CCEae3a) and 6 (CCEae6a) gene amplification associated with organophosphate (OP) resistance and; chitin synthase-1 (CHS-1) for the possible presence of DFB resistance mutations. Results: Bioassays showed full susceptibility to DFB, Bti and deltamethrin, but resistance against the OP malathion. VGSC analysis revealed a widespread distribution of mutations F1534C (in all populations, with allelic frequencies between 6.6% - 68.3%), and I1532T (in 6 populations), but absence of V1016G. CCE gene amplifications were recorded in 8 out of 11 populations. Co-presence of mutation F1534C and CCEae3a amplification was reported in a subgroup of samples. No mutations at the CHS locus I1043 were detected. Conclusions: The results indicate: (i) the suitability of larvicides DFB and Bti for Ae. albopictus control in Greece, (ii) a possible incipient pyrethroid resistance due to the presence of kdr mutations and (iii) a possible reduced efficacy of OPs, in a scenario of re-introducing them for vector control. The study highlights the need for systematic resistance monitoring for developing and implementing appropriate evidence-based control programs. Key words: diagnostic, arbovirus, mosquito tiger, insecticide resistance, vector control, Europe
Theoretical Molecular Docking Study of the Structural Disruption of the Viral 3CL-Protease of COVID19 Induced by Binding of Capsaicin, Piperine and Curcumin Part 1: A Comparative Study with Chloroquine and Hydrochloroquine Two Antimalaric Drugs
Authors: Gonzalez-Paz Lenin A. , Lossada Carla A. , Moncayo Luis S. , Romero Freddy , Paz J. L. , Vera-Villalobos Joan , Pérez Aleivi E. , San-Blas Ernesto , Alvarado Ysaias J.
Published: April 2020

The global pandemic caused by infections of the new coronavirus (COVID-19) makes it necessary to find possible less toxic and easily accessible therapeutic agents. In this study, we used strategies docking and molecular dynamics to analyze phytochemical compounds against FDA-approved antimalarial drugs recommended for the treatment of COVID-19. The evaluation was performed with the docking scores MolDock Score and Rerank Score calculated by Molegro Molecular. The DockThor server was used to generate the complexes and myPresto for the dynamic studies. Preliminary results suggested that piperine, capsaicin, and curcumin have the best docking scores and that they are capable of promoting structural changes in the viral protease by inducing folding of the enzyme. Curcumin and capsaicin bring the enzyme to a more compact conformational state compared to the native state, compared to chloroquine. Even though, it is unknown if these induced changes in protease are related to any inhibitory effect observed both in vitro and in vivo for any of these compounds. Further studies on the mechanisms of action of these compounds of interest are required, as well as experimental demonstrations. However, these results are interesting because they can serve as a starting point for subsequent experimental or/and in silico studies based on chemical structure-activity relationships taking these small molecules and their possible derivatives.
From sequence data to patient result: a solution for HIV drug resistance genotyping with Exatype, end to end software for Pol-HIV-1 Sanger based Sequence analysis and patient HIV drug resistance result generation
Authors: Kingwara Leonard , Karanja Muthoni , Ngugi Catherine , Kangogo Geoffrey , Bera Kipkerich , Kimani Maureen , Bowen Nancy , Abuya Dorcus , Oramisi Violet , Mukui Irene
Published: April 2020

Introduction: With the rapid scale-up of antiretroviral therapy (ART) to treat HIV infection, there are ongoing concerns regarding probable emergence and transmission of HIV drug resistance (HIVDR) mutations. This scale-up has to lead to an increased need for routine HIVDR testing to inform the clinical decision on a regimen switch. Although the majority of wet laboratory processes are standardized, slow, labor-intensive data transfer and subjective manual sequence interpretation steps are still required to finalize and release patient results. We thus set out to validate the applicability of a software package to generate HIVDR patient results from raw sequence data independently.Methods: We assessed the performance characteristics of Hyrax Bioscience's Exatype (a sequence data to patient result, fully automated sequence analysis software, which consolidates RECall, MEGA X and the Stanford HIV database) against the standard method (RECall and Stanford database). Exatype is a web-based HIV Drug resistance bioinformatic pipeline available at sanger.exatype.com. To validate the exatype, we used a test set of 135 remnant HIV viral load samples at the National HIV Reference Laboratory (NHRL).Result: We analyzed, and successfully generated results of 126 sequences out of 135 specimens by both standard and Exatype software. Result production using Exatype required minimal hands-on time in comparison to the standard (6 computation-hours using the standard method versus 1.5 Exatype computation-hours). Concordance between the two systems was 99.8% for 311,227 bases compared. 99.7 % of the 0.2% discordant bases, were attributed to nucleotide mixtures as a result of the sequence editing in Recall. Both methods identified similar (99.1%) critical antiretroviral resistance-associated mutations resulting in a 99.2% concordance of resistance susceptibility interpretations. Base calling comparison between the two methods had Cohen's kappa (0.97 to 0.99) implying an almost perfect agreement with minimal base calling variation. On a predefined dataset, RECall editing displayed the highest probability to score mixtures accurately one vs. 0.71 and the lowest chance to inaccurately assign mixtures to pure nucleotides (0.002–0.0008). This advantage is attributable to the manual sequence editing in RECall.Conclusion: The reduction in hands-on time needed is a benefit when using the Exatype HIV DR sequence analysis platform and result generation tool. There is a minimal difference in base calling between Exatype and standard methods. Although the discrepancy has minimal impact on drug resistance interpretation, allowance of sequence editing in Exatype as RECall can significantly improve its performance.
Analysis of clinical characteristics of SARS-CoV-2 infected cases: a retrospective study of medical records
Authors: Zhang Han , Lu Lian , Hu Wei , Zhang Jian , Zhu Wei , He Qi-Qiang , Sun Cheng-Cao , Li De-Jia
Published: April 2020

In order to identify the clinical characteristics of patients with Corona Virus Disease 2019 (COVID-19) and find out the characteristic effects of 2019 New Coronavirus (SARS-CoV-2) infection on changes in clinical and laboratory data, we analyzed the medical records of 80 suspected cases who admitted in the national designated hospital due to the relevant clinical manifestations of SARS-CoV-2 infection from January 22 to February 13, 2020. 62 (77.5%) confirmed cases and 18 (22.5%) negative cases were confirmed by SARS-CoV-2 nucleic acid test. Epidemiological investigation and statistical analysis were carried out on the clinical and laboratory data of all suspected cases of COVID-19, the specific indicators were found, and the clinical characteristics of COVID-19 were described. Compared with the patients with negative nucleic acid test, the patients with positive nucleic acid test showed shorter time of onset of symptoms, higher plasma CO2 level, lower eosinophil ratio, lower platelet count and hematocrit, lower serum sodium level, higher serum creatinine, higher blood urea and plasma albumin levels (all P<0.05). Our results might provide some suggestions in diagnosis, clinical treatment and prevention for COVID-19.
Detection and Identification of Microbial Contaminant in Bakery Products in Yogyakarta City, Indonesia
Authors: Ferdian Evelyn , Ariestanti Catarina Aprilia , Budiarso Tri Yahya
Published: April 2020

Bread has been chosen as an alternative food because of its availability. In Indonesia, consumptionof breads is increased due to the change of diet pattern into packed and ready-made meals. Therefore, it isimportant to raise the awareness of bakery products quality. The purpose of this study was to detect andidentify the microbial contaminant in bakery products in Yogyakarta City, Indonesia. Bacterial coloniesfrom expired bakery products were isolated into pure isolate then confirmed by API Staph and PolymeraseChain Reaction (PCR) method. The results showed there were five kinds of gram-positive bacteria. Isolatedbacteria identified by API were Bacillus cereus (52.8%), Bacillus subtilis (97.7%), Staphylococcus aureus (97.7%),Staphylococcus epidermidis (97,9%) and Staphylococcus saprophyticus (72,2% and 61,8%).
Synthesis of Biodiesel from Kesambi Oil using Indigenous Lipase
Authors: Ledo Mellisa E.S. , Nitsae Merpiseldin , Solle Hartini R.L.
Published: April 2020

Kesambi (Schleichera oleosa L.) is one of ligneous tree that is abundantly found in Timor island, East Nusa Tenggara Province of Indonesia. Kesambi is known as an important component in local smoked meat home industry. Kesambi is also a promising natural source for the production of biodiesel. Application of three lipolytic fungi that is isolated from kesambi seeds and the use kesambi seeds flour as medium for indigenous lipase synthesis (Aspergillus niger M1407 lipase) by Solid-State Fermentation (SSF) showed good result for lipase and biodiesel industry development. This study aimed to characterize biodiesel synthesis using indigenous lipase from kesambi seeds. Experiments were carried out through five stages namely: oil extraction from kesambi seeds, purification of kesambi oil (degumming), extraction of A. niger M1407 lipase, trans-esterification and esterification activity of A. niger M1407 lipase assay and characterization of biodiesel. Biodiesel synthesis using A. niger M1407 lipase through esterification reaction produced biodiesel with an average acid number of 0.33 and 0.40 mg/g, water content 9.52 % and 10.47 % and density 0.7 g/mL. Biodiesel obtained from kesambi oil has lower acid numbers and densities but higher water content compared to Indonesian National Standard (SNI) value. Optimalization of the biodiesel kesambi oil synthesis could be done through adjusting ethanol and kesambi oil ratio and also by reducing water as a by-product of esterification reaction
The Fröhlich-Morchio-Strocchi mechanism and quantum gravity
Authors: Maas Axel
Published: April 2020

Taking manifest invariance under both gauge symmetry and
diffeomorphisms as a guiding principle physical objects are constructed
for Yang-Mills-Higgs theory coupled to quantum gravity. These objects
are entirely classified by quantum numbers defined in the tangent space.
Applying the Fröhlich-Morchio-Strocchi mechanism to these objects
reveals that they coincide with ordinary correlation functions in
quantum-field theory, if quantum fluctuations of gravity and curvature
become small. Taking these descriptions literally exhibits how quantum
gravity fields need to dress quantum fields to create physical objects,
i. e. giving a graviton component to ordinary observed particles. The
same mechanism provides access to the physical spectrum of pure
gravitational degrees of freedom.
Formulation and Evaluation of a Novel Itraconazole-Clotrimazole Topical Emulgel for the Treatment of Sporotrichosis
Authors: Noronha Letícia L. , Ferreira Patricia G. , G.S. Lima Carolina , Borba-Santos Luana P. , Rozental Sonia , de Moraes Marcela , Silva Fernando C. da , Ferreira Vitor F. , Futuro Débora O.
Published: April 2020

:

In recent years, the development of new pharmaceutical formulations for the treatment of sporotrichosis
has become a relevant research field. In this work, we aimed to develop an emulgel containing itraconazole
and clotrimazole to ensure therapeutic effectiveness against Sporothrix brasiliensis. The topical use of a formulation
that combines both drugs represents an interesting option for the complementary treatment of sporotrichosis.
The emulgel formulation was prepared and evaluated for its zeta potential, viscosity, in vitro antifungal activity
and stability at different storage conditions. The results showed that the newly developed emulgel displayed
promising physicochemical characteristics, as well as a good in vitro inhibitory activity against S. brasiliensis
yeasts. The results obtained in this work suggest that the emulgel containing itraconazole and clotrimazole might
highly be efficient and a complementary therapy to oral administration in the treatment of sporotrichosis.
Synthesis and Antimicrobial Activity of Novel 1, 2, 4-Triazolopyrimidofuroquinazolinones from Natural Furochromones (Visnagenone and Khellinone)
Authors: Abu-Hashem Ameen A. , Hussein Hoda A.R. , Aly Ahmed S.
Published: April 2020

Background:

Previous and recent scientific research has shown that triazolopyrimidine and furochromones have a wide range of pharmacological activities for the treatment of numerous diseases, including anticancer, antiviral, anti-depressant, anti-microbial, anti-inflammatory, and analgesic activities.

Objective:

Preparation of new drugs derived from a natural furochromones as (1-hydrazinyl or methylthio),-furopyrimidoquinazolinone, 1, 2, 4-triazolopyrimidofuroquinazolin-5-one, and quinazoline- pyrimidofuro- quinazoline-8, 10-dione and the study of their biological activity as antimicrobial agents.

Method:

A series of novel N’-furopyrimidoquinazoline-hydrazide; 1, 2, 4-triazolopyrimidofuroquinazolin- 5-one; furopyrimidoquinazolin-3-one and quinazoline-pyrimidofuroquinazoline-8, 10- dione derivatives were synthesized from substituted (methylthio)-furopyrimidoquinazolinone (3ab) and 1-hydrazinyl-furopyrimido- quinazolinone (4a-b) as the starting material.

Results:

All compounds were synthesized in good yields (71-95%) in a gradually efficient system under mild condition and some of the procedures were used such as microwave oven. The new compounds have been confirmed by means of different spectroscopic methods such as IR, 1D and 2D -NMR techniques and mass spectrum. The in vitro antimicrobial activities were evaluated for the prepared compounds using many types of bacteria (Gram-positive and Gram-negative) and fungi.

Conclusion:

1, 2, 4-triazolopyrimidofuroquinazolin-5-one derivatives (10a-f, 8a-b, 7a-b and 6a-d) showed the most efficient antimicrobial activities compared with the cefotaxime sodium and nystatin as standard drugs.
An Occupational safety and health (OSH) factors identified in Indonesian batik textile small/medium enterprises
Authors: Apsari Ayudyah Eka , Purnomo Hari
Published: April 2020

Indonesia is a country with numerous SMEs spread throughout 34 provinces. One of the important problems that need to consent more is labor health and safety. To increase safety, SMEs need to identify factors of occupational health and safety. SMEs need to properly avoid workplace accidents, and the batik industry is no exception. As a first step, it is necessary to identify the OSH factors that can be used in a prevention accident strategy. The population in this study were workers in Indonesia, these batik companies were located in Solo, Yogyakarta, Pekalongan, Cirebon, Minahasa, Bali, Madura, Indramayu, Bengkulu, and Palembang. The questionnaire consisted of latent variables, from the research that has been done, variables that affect work safety are the physical work environment, social work environment, job stress, and work productivity, while the variables that have a significant influence on occupational health are the physical work environment, social work environment, and work stress.
Ensino de Artes Visuais: histórias e memórias
Authors: Azevedo Fernando Antônio Gonçalves de , Alves Flávia Leal
Published: April 2020

Apresentaçao
Anita, Mário e a modernidade da arte/educação brasileira
Authors: Coutinho Rejane Galvão
Published: April 2020

O artigo ressalta as contribuições de Anita Malfatti e Mário de Andrade para a modernidade da arte/educação brasileira, colocando em evidência o plano de curso de desenho para crianças deixado por Anita, que é comentado e posto em relação com a coleção de desenhos infantis de Mário de Andrade. Trata-se de uma livre interpretação de parte da história do ensino de artes no Brasil tendo como referências principais as pesquisas de Ana Mae Barbosa e Marta Rossetti Batista.
O fazer sensível de Nelma Pezzin: artista e professora
Authors: Rebouças Moema Martins
Published: April 2020

Este texto tem como objetivo principal recuperar práticas artísticas e docentes para interrogar como a poeticidade de um fazer sensível atua e faz sentido. Tem como hipótese que a Arte e os processos artísticos nutrem a docência e instituem metodologias e práticas educativas em contextos de ensino. Elege como corpus uma das 10 artistas integrantes da pesquisa “O lugar do discurso na Arte e na Docência: entrelaçamentos e articulações tecidas em contextos educativos”, que foram professoras no Centro de Artes da Universidade Federal do Espírito Santo (UFES), desde o ano de 1976. Utiliza Duve como referencial para o ensino superior e Fiorin, Landowski, Oliveira para as análises na abordagem semiótica. Conclui que as relações entre a Arte e o ensino são de trocas e a educação é partilha. A “forma de vida” da professora artista, ou o que a faz-ser tem na articulação das práticas artísticas e docentes a sua própria constituição como sujeito.
Music, Markets and Consumption
Authors: O’Reilly Dr Daragh , Larsen Dr Gretchen , Kubacki Dr Krzysztof
Published: April 2020

A fully international and scholarly analysis integrating the unique popular music sector both within arts marketing and current marketing and consumption theories. Music, Markets and Consumption offers an up-to-date business-theoretical reading of the music business which complements viewpoints from other disciplines. It will be a much needed new perspective for students and scholars in music studies, cultural studies, marketing and consumer studies who wish to gain further insight into commercial aspects of music.
OS JOGOS MATEMÁTICOS E O JOGO “1º GRAU OU GRAU?”
Authors: Francisquini Barreto Laísa , Gava Alessio
Published: April 2020

O presente artigo irá discorrer sobre o uso de jogos para o ensino da matemática, em busca de um aprendizado mais efetivo e consistente dos conceitos dessa disciplina. Em particular, será apresentado o resultado de uma oficina aplicada em turmas do segundo e do terceiro ano do ensino médio do colégio estadual “Heitor Cavalcanti de Alencar Furtado” (Apucarana, PR). Nela, foi utilizado um jogo criado pela autora do presente trabalho,“1º grau ou grau?”, que envolve dois tópicos importantes do conteúdo programático de matemática do ensino médio: às equações de primeiro grau e a trigonometria. O objetivo da oficina foi trabalhar esses dois conceitos de maneira lúdica e colaborativa, mas também verificar se esse pode ser ao mesmo tempo um modo eficaz e produtivo de desenvolver o processo de ensino-aprendizagem da disciplina. Pelas observações das professoras que supervisionaram a atividade e pelas respostas que os discentes forneceram em um questionário aplicado posteriormente, concluimos que os jogos matemáticos constituem de fato um instrumento precioso para os docentes, capaz de cativar a atenção e o interesse dos estudantes e de auxiliar esses últimos na aquisição de noções essenciais da disciplina.
A tributação sobre o consumo de combustíveis de Uberlândia - MG e a aplicabilidade da Lei 12.741 de 2012
Authors: Morais Luiz Fernando
Published: April 2020

O objeto de estudo da pesquisa foi a tributação sobre o consumo de combustíveis de Uberlândia – MG e a aplicabilidade da Lei 12.741/2012. Desta forma o problema da pesquisa foi demonstrar qual a aplicabilidade da lei de Transparencia Fiscal sobre o consumo de combustíveis. As hipóteses da pesquisa foram que a maioria dos estabelecimentos utilizam como fonte o IBPT (Instituto Brasileiro de Planejamento e Tributação) e um mesmo produto pode apresentar cargas tributárias diferentes. Sendo assim o objetivo da pesquisa foi analisar a aplicabilidade da Lei “De olho no imposto” e seus impactos no consumo de combustíveis. O método de pesquisa foi uma pesquisa documental de coleta de dados de cupons fiscais em Postos de Combustíveis de Uberlândia referente ao período de janeiro á maio de 2016. A amostra foram extraídas do total de 22 estabelecimentos de combustíveis. A análise dos dados foi baseada no referencial teórico do IBPT e textos legais (legislação específica). Os resultados encontrados indicam que a maioria dos estabelecimentos não aplicam a lei 12.741/2012 corretamente, informando carga tributária incorreta em relação á fonte indicada. Conclui-se que a aplicabilidade da lei não é eficiente e eficaz, pois as informações ao consumidor é distorcida.
The Nexus Between Government Revenue and Economic Growth in Nigeria
Authors: Joseph Fineboy Ikechi , Omodero Cordelia Onyinyechi
Published: April 2020

The aim of the study is to examine the relationship between government revenues and the economic growth of Nigeria. The study employs exploratory and ex-post facto research designs while using secondary form of data spanning from 1981 to 2018 collected from the Federal Inland Revenue Services (FIRS), National Bureau of Statistics and CBN statistical bulletin. The relationship is tested by using Ordinary Least Squares (OLS) regression technique. The result reveals that federally received revenue and Value Added Tax (VAT) have a moderate positive relationship with the economic growth. The study provides evidence that there is a need for the government to formulate relevant revenue policies that will boost government income in order to have more favourable implication on the economy.
‘Vex the devil’: Scripture, God-talk and holiness at Villa Road
Authors: Smith Jennifer H.
Published: April 2020

This article gives a critical account of the engagement with Scripture among a group of women and men from the Villa Road Methodist Church, Handsworth, Birmingham, UK. It presents research done in community during 2004/05 for the unpublished thesis, ‘Mary in the Kitchen, Martha in the Pew: Patterns of Holiness in a Methodist Church’ (MPhil., Birmingham, 2006), updated to include critical engagement with contemporary scholarship in Black British theology, womanist and feminist theology, holiness teaching and hermeneutics, and congregational studies. Working from ethnographic research, the article considers three clusters of emphasis, or creative tensions, in the use of Scripture: gender and God, thanksgiving/resistance in response to evil, and displacement/home-coming. The article argues that these themes specially concern the negotiation of identity and relationship between self, God, Scripture and context.
Numerical Simulation of Heat Transfer Behavior of Dissimilar AA5052-AA6061 Plates in Fiction Stir Welding: An Experimental Validation
Authors: Vishwanath M. M. , Lakshamanaswamy N. , Ramesh G. K.
Published: April 2020

Fiction Stir welding (FSW) a unique type of metal joining process in solid state, where the heat generation takes place due to the friction action between the rotating tool and the work piece. It is generally used to join all series of Aluminum alloys with good strength and other metallic alloys finds difficult to weld through regular fusion welding techniques. The metal joining takes place in the solid state as the metal to be welded reaches about 80% to 90% of its melting temperature. The joining of metals in friction stir welding does not require any filler metals all classes of Aluminium alloys can be joined and in some desirable cases dissimilar metal compositions and Aluminiun metals composites can be joined satisfactorily. Joining of dissimilar metals has become a trend in the industries like aerospace, automotive chemical etc. as the helps in reducing the cost incurred by eliminating the costly materials. In the present study an experimental investigations are made to study the heat transfer behavior by determining the temperature distribution in AA5052-AA6061 plates during the Friction Stir welding. A three dimensional transient analysis is made by using ANSYS finite element analysis software. Thermocouples are placed at the suitable locations and the same point the temperature readings were taken from the simulation results. The experimental data is compared with the numerically simulated results. The numerical simulations results obtained are in better agreement with the experimental data obtained.
Research into behavioral traits of the susceptibility to the risk of significant financial misstatements
Authors: Arzhenovskii S.V. , Sinyavskaya T.G. , Bakhteev A.V.
Published: April 2020

Subject. The article identifies behavioral signs of the susceptibility to the risk of material misstatements through the expert survey of professional auditors.
Objectives. We do empirical research into the impact five behavioral traits have, which we discovered through the two parameter risk assessment model, i.e. tolerance to violation of laws, money pathology, susceptibility to high risk, aspiration of impunity and legislative illiteracy in finance.
Method.s We performed the expert survey of professional auditors to discover what determines the susceptibility to fraud among those charged with financial reporting. The expert group was made on the basis of an unbiased approach and documentation. We applied the Rasch model to rank personal traits. The collected data were processed with methods of descriptive statistics and multivariate statistical analysis.
Results. Carrying out the statistical analysis of experts’ opinions, we found that their significantly correlated. Personal traits were sorted by their impact on risk assessment. Money pathology, susceptibility to high risk, aspiration of impunity and legislative illiteracy in finance were acknowledged as the most influential factors in terms of the susceptibility to misstatements of financial reporting.
Conclusions and Relevance. We empirically proved the importance of factors influencing the propensity to risk of misstating financial reports. We used our own theoretical concept. The findings can be useful to auditing forms to detect the customers’ propensity to the risk of manipulating financial reporting.
Tax capacity in the system of fiscal federalism and promotion of regional development
Authors: Ermakova Yu.S.
Published: April 2020

Subject. The article discusses the role of tax capacity in the management of inter-budget relationships.
Objectives. The article aims to build and develop a qualitatively new motivational model to encourage Russian constituents to increase their tax potential by ranking them in socio-economic development groups.
Methods. For the study, I used the methods of comparative, vertical and horizontal analysis, and statistical groupings.
Results. The article clarifies that the mechanism for providing financial or other support to regions with the best tax potential is not working well because the economically developed territories have a higher rate of tax capacity and will always be ahead of the less developed ones. However, that could not be a significant growth rate in the main macroeconomic indicators, since a certain level of development has already been achieved.
Conclusions. The article reckons that it is necessary to rate the regions by group of social and economic development and identify leaders with the best tax potential value in each group. This can help to develop an effective system of budgetary allocation and stimulating the regions' tax potential development.
The methodological apparatus for planning and regulating trends in high-tech production costs under risk
Authors: Leonov A.V. , Pronin A.Yu.
Published: April 2020

Subject. The article discusses the methodological apparatus for assessing how many financial resources are needed, and optimize their gradual consumption in high-tech production projects. Results of the projects can be forecasted only as a probable estimate, since there is great uncertainty due to a multitude of random factors.
Objectives. The study aims to form the methodological apparatus to assess the amount of financial resources needed under risk and substantiate the reasonable strategy for consuming them in high-tech production projects within a given period of time. The apparatus is to allow for quick adjustments of the financing plan and estimation of project expenditures.
Methods. We applied the comprehensive approach to planning and regulating trends in high-tech production costs, methods of economic and mathematical modeling and the probability theory.
Results. We reviewed methods used to assess how many financial resources are needed through demand probability distribution laws. Based on them, we devised the interval technique for regulating cost trends so as to substantiate the reasonable strategy for the performance of projects with the desired probability within the given period of time. What distinguishes the interval technique is that it provides the overall vision of the period, during which the project will be performed, and the probability of the advanced prediction of a shortage or excess of financial resources.
Conclusions and Relevance. The methodological apparatus proposed herein will facilitate the technological and economic evaluation of various options of high-tech production and choose those ones which ensure the best use of financial resources, quickly regulate the economic dynamism throughout the high-tech production phases in line with a variety of factors, which randomly emerge at certain phases.
The working conditions of the substitute university professor in contemporary times: exploring the reality of a public university
Authors: Silva Maiara Lopes da , Maia Filho Osterne Nonato , Rabelo Josefa Jackline
Published: April 2020

The present study aims to discuss the precariousness of the teaching labor conditions in contemporary capitalism, particularly addressing the status of the substitute teacher in the magisterium career on academic education. The nature of the study is qualitative and exploratory, based on documentary analysis and interview, due to its approximate bias to the theme, being guided by the theoretical-methodological perspective of historical-dialectical materialism and Marxist ontology. The main results of our investigation indicate that substitute teachers are submitted to visible conditions of devaluation of their teaching activities. His work, marked by transience, almost becomes a necessary requirement for his future hiring as an effective public teacher. In this sense, the teachers are subordinated to receive a remuneration that, in general, is well inferior compared to effective teachers. Their activities are distinguished by the “aulism”, instead of the research and extension, which, in addition to teaching,also compose the basic social functions of the university, whose reference is the production of knowledge and its application.
“Educating for alterity”: Shoah History Teaching and use of USC Shoah Foundation audiovisual testimonies
Authors: Palmeira Alyne Nathálier da Silva , Schurster Karl
Published: April 2020

This work, in the theoretical light of the History of Present Time, proposed to construct an analysis of the History Teaching of the Shoah, more specifically from the audiovisual materials of the USC Shoah Foundation Institute for Visual History and Education, expanding the Brazilian academic research in the field of studies called “Teaching Pedagogy of Collective Traumas”. We will try to discuss the set of materials chosen, which include testimonies of survivors of the Shoah, appropriating a content analysis. It was established what these materials elucidate on the teaching of this subject, problematizing its methods and objectives, as well as about the proper role of the teacher as a tool of this teaching. From this perspective, Shoah's History Teaching establishes itself as a possible tool in the fight against fascism, in the negation of revisionist theories, and in the understanding of a new worldview about the moral, ethical and juridical values of society in the post-war.
Permanent formation of tutors: a study inspired in Paulo Freire with teachers of early years of basic education
Authors: Soares Maria Perpétua do Socorro Beserra
Published: April 2020

This article presents the results of a research about permanent formation of teachers of early years of fundamental teaching of a public district school of a city of São Paulo-SP; whose general objective is to analyze scopes and limits of permanent formation of teachers to a pedagogical practice committed to building a democratic school. The theoretical contributions is based on the pedagogy of Paulo Freire (2001, 2013b, 2015, and 2016), Imbernón (2009, 2010), and Saul (2010). The nature of the investigation is qualitative and follows Chizzotti (2005) with bibliographic study, field and documentary research. The results of the research show a strong presence of dimensions of permanent formation; materializing principles as the hearing, the dialog, the participation and the movement of action-reflection-action, demonstrated on documents, interviews done, observations, formation meetings and parent-teaching conference.
Criminogenic role of cryptocurrency
Authors: Korchagin Anatolii Georgievich , Yakovenko Andrei Aleksandrovich
Published: April 2020

  This article explores the phenomenon of cryptocurrency and technology it is based upon. The authors describe the mechanism of its functionality along with the occurred problems of legal nature, which being interrelated make the phenomenon in question appealing within the criminal environment. Global digitalization sets new requirements, namely the combinations of legal and technical regulators meant to achieving adequate legal regulation in the digital era. The subject of this research is the Russian and foreign legal doctrine that reveals the essence of the indicated technology and mechanisms for protecting social relations in the context of utilization of this technology. The research methodology consists of the following methods: statistical, dogmatic, comparative-legal, synergetic, logical, functional, and systemic. The scientific novelty is substantiated by the need for convergence of the legal and information systems and demonstration of such convergence during the period of rapid digitalization in all social spheres. The authors draw a conclusion that the problem carries a comprehensive character, and the longer it takes to resolve the issues of legal regulation, the higher are the risks of using cryptocurrency.  
SAMPL7 TrimerTrip Host-Guest Binding Poses and Binding Affinities from Spherical-Coordinates-Biased Simulations
Authors: Sun Zhaoxi
Published: April 2020

Host-guest binding remains a major challenge in modern computational modelling. The newest 7th statistical assessment of the modeling of proteins and ligands (SAMPL) challenge contains a new series of host-guest systems. The TrimerTrip host binds to 16 structurally diverse guests. Previously, we have successfully employed the spherical coordinates as the collective variables coupled with the enhanced sampling technique metadynamics to enhance the sampling of the binding/unbinding event, search for possible binding poses and predict the binding affinities in all three host-guest binding cases of the 6th SAMPL challenge. In this work, we employed the same protocol to investigate the TrimerTrip host in the SAMPL7 challenge. As no binding pose is provided by the SAMPL7 host, our simulations initiate from randomly selected configurations and are proceeded long enough to obtain converged free energy estimates and search for possible binding poses. The predicted binding affinities are in good agreement with the experimental reference, and the obtained binding poses serve as a nice starting point for end-point or alchemical free energy calculations.
Solving an X-Ray Structure of a Bio-Artificial Neurotransmitter Receptor Designed for the Naked-Eye Recognition of Dopamine
Authors: Rossel Thibaud , Zhang Bing , Gobat Raphael
Published: April 2020

The literature is with a wide variety of against a plethora of . This seminal library is used to inspire chemists to improve them using chemical synthesis. However, their optimization via chemical synthesis is a difficult task which takes time without the guarantee of final success. We show here that chemistry, the use of first and second coordination spheres and the displacement of indicators united within a protein cavity offers an easy-to-assemble bio-chemical sensor. It consists only of commercial chemicals. This sensor is highly modular, cheap and . Its X-ray structure reveals the composition of its active site. This allows to design it rationally for the recognition of dopamine with the naked eye. Our bio-sensor therefore resembles a biological receptor for the recognition of neurotransmitters. Its immediate high adaptability and ability to be evolved can be useful for the selective detection of a wide variety of going from small molecules to microorganisms. This discovery therefore makes it possible to dream of new biotechnological or new applications.
ADMINISTRATIVE AND LEGAL ASPECTS OF LICENCING IN HIGHER EDUCATION
Authors: Sysojev Oleksij
Published: April 2020

The study provides an insight into administrative and legal aspects of licensing higher educational institutions, analyses changes in the licensing system of higher educational institutions and examines measures aimed at nhancing the role of this institution in addressing the problem of quality assurance of higher educational institutions. The author investigates the legal nature and administrative aspects of licensing in higher education and proves that licensing in higher education is an administrative procedure, which takes into account the specific nature and characteristics of the activities of an educational institution, that are stipulated by the corresponding regulatory documents. The paper determines licensing as a legal phenomenon, which means that licensing activity belongs to the system of state-executive relations, the core of which is the organizing the citizens and the legal entities’ activities in the spheres where steadfast adherence to definite requirements and certain legal behavior is expected. One of the key research questions of this paper was the ambiguity of the concept of licensing in scientific literature, which combines the norms of administrative and civil law. Social and legal functions of licensing higher education institutions with the aim of their effective functioning in social and legal environment are outlined. Further, the author identifies the characteristic features of licensing in education.
This article also address the research questions of licensing in education and offers the conclusions regarding the features of licensing as a legal phenomenon. The role of licensing activity in the system of state-executive relations and its contents and constituents are determined.
ACADEMIC MOBILITY OF UKRAINIAN STUDENTS IN THE HISTORICAL RETROSPECTIVE: BEGINNINGS, DIRECTIONS, MOTIVES
Authors: Suchyk Oleksandr , Suchyk Iryna
Published: April 2020

The article reveals the phenomenon of academic mobility of students, shows its genesis, trends and flows of mobile youth during historical periods. The attention was paid to the motivational component of the process of youth integration into the global educational space. The main periods of the academic mobility of students from the medieval origins to the present are highlighted.
The first Western European universities are shown by the international intellectual centers of Europe XIV-XVII, where the leading youth of those times went. It is noted that the wandering lifestyle of medieval students was a way of integrating into the world of educational space. The attention is paid to the emergence of Ukrainian youth in European universities. The data on the number of Ukrainian students, their adaptation to the new cultural and educational society is given. Names of Ukrainians, graduates of European universities that became known to the world are mentioned.
The need for mobility, as moved to find a better option and learning outside of their own intellectual environment, has been traced in different historical periods. Changes in the global picture of the movement of mobile students from the late 90’s of the XX century. The attention is focused on the modern problem of immigration of progressive Ukrainian youth, the reasons for the migration of students, the main centers of their accumulation are named.
The main obstacles to promoting academic mobility of students to the European and world educational communities are considered. The emergence of virtual universities as an alternative to traditional appears, which become the basis of virtual academic mobility of the present.
Implementation of Thoharoh in early childhood through Telling Story in Babadan Village
Authors: Rahmawati Lilis
Published: April 2020

Thoharoh became the basis of a practice of worship, as it became the condition of worship.  So it is necessary to be taught since early childhood is good and correct. Since early childhood is the Golden Age. This research aims to discuss the implementation of education in Thoharoh in early childhood in Babadan village. Using a descriptive method is a clear picture of delivering, presenting, and concluding data. Population retrieval and samples from the research object as a tool to obtain data from an issue that is also required by the authors. As for digging data, the methods used are documentation methods, interviews, polls and to know the type of data using qualitative data. His study was done very impressively through various fun games, demonstrations and Thoharoh festivals. The results showed that Thoharoh could be implemented early on through a pleasant method. With the hope that parents participate in this religious education so that the child can practice it optimally.
Addressing the Unmet Needs of Measuring Vascular Ageing in Clinical Practice—European COoperation in Science and Technology Action VascAgeNet
Authors: Climie Rachel Emma , Mayer Christopher Clemens , Bruno Rosa Maria , Hametner Bernhard
Published: April 2020

Cardiovascular Disease (CVD) is the leading cause of morbidity and mortality worldwide, regardless of gender, ethnicity or income. Evidence that vascular age, as opposed to chronological age, is better related to the prognosis of CVD is convincing. Despite recent technological advances for the measurement of vascular ageing, unmet needs remain which currently limit the assessment of vascular ageing in routine clinical practice. These unmet needs include: (1) lack of easy-to-use, affordable techniques/devices, (2) insufficient validation in clinical settings, (3) fragmentation of expertise in few research laboratories globally, and (4) lack of investigator/research driven intervention studies and head-to-head comparisons between different measurement techniques. This paper summarises how a recently awarded European COoperation in Science and Technology (COST) Action on Vascular Ageing (VascAgeNet) will address these unmet needs. The broad aim of the COST Action VascAgeNet is to establish a network which will work to refine, harmonize and promote the use of vascular ageing biomarkers, in order to improve clinical practice and to reduce the burden of CVD globally.
Best Determined Position of Vents Based on Jet Cooling Model
Authors: Huang Na
Published: April 2020

In some data centers, cold air is required to act on the cabinet to achieve cooling requirements, and the mixing of cold air and hot air reduces the utilization efficiency of cold air. In order to solve this problem, a jet cooling model is established to solve the optimal position of the outlet through the movement of cold air.
EFFECT OF COUNSELLING GUIDANCE IN IMPROVING PROCESS OF STUDENT LEARNING ACHIEVEMENT IN MTS NEGERI I BOJONEGORO
Authors: Asrori Asrori
Published: April 2020

Quality education is one that integrates several fields of activity in synergy namely administrative, instructional and guidance and counselling. This research aimed to transform the knowledge into the education system integrally. The type of research used is field research (field resources) with a quantitative approach. In this research, there were two variables, namely Guidance and Counselling as independent variables, and student achievement as a dependent variable. The researcher conducted a study in MTs Negeri I Bojonegoro and the researchers selected grade 7 students as population, and assigned 10% of them as samples. Techniques in sampling in the form of random sampling. The source of this research data obtained by the method of observation, interview, questionnaire and documentation. The results of this research showed that counselling and guidance services affect the process of improving student achievement. This is based on the questionnaire score data that has been calculated with the product moment formula that results in a correlation index value (rxy) of 0.681. This result proved larger than the value at the significance level of 5% of 0.361 and 1% of 0.463. The results of this study indicate that there is influence between Counselling Guidance on the process of improving student achievement in MTs Negeri I Bojonegoro.
A Study on Student Attitudes in Learning Programming using Physical Computing
Authors: Zaini Zuraini Hanim , Arshad Noreen Izza , Singh Balbir SN , Aszemi Nurshazlyn M , Anggoro Subuh , Hawanti Santhy
Published: April 2020

Learning to program can be difficult for the students. Students must master language syntax, programming theory, and problem-solving techniques. Efforts have been made to assist students in understanding how to program. This study is intended to examine whether Arduino, as a teaching and learning tool, helps in generating students’ interests towards programming. Arduino is one of the physical computing tools which has an open-source electronics platform based on user-friendly hardware and software for creating different projects and applications. Arduino is easy to be used by beginners, yet flexible enough for advanced users to learn physical computing and programming. This study adopted a quantitative research method to measure the student’s attitude in learning programming using physical computing. The sample of this study is 56 students from the foundation program and undergraduate program. To gauge students’ perception, students’ attitude survey was adapted. The collected data were analyzed using descriptive analysis. Based on the analysis, the study found that the overall mean score was 4.253. The result indicated that student has a positive attitude in learning programming using physical computing.