Sulfoconjugated and free plasma catecholamine levels at rest and during exercise in patients with idiopathic dilated cardiomyopathy
Authors: Dobnig Harald , Leb Georg , Lipp Rainer , Porta Sepp , Dusleag Johann , Eber Bernd , Klein Werner , Krejs Guenter J 
Published: December 2008

Dobnig H, Leb G, Lipp R, Porta S, Dusleag J, Eber B, Klein W, Krejs GJ. Sulfoconjugated and free plasma catecholamine levels at rest and during exercise in patients with idiopathic dilated cardiomyopathy. Eur J Endocrinol 1995;132:181–91. ISSN 0804–4643

Plasma levels of sulfoconjugated (sc) catecholamines (CA) have been shown to be increased with activation of the sympathoadrenal system in a number of clinical settings. We evaluated the relation between scCA and clinical or hemodynamic parameters of patients with idiopathic dilated cardiomyopathy (IDC) at rest and during incremental exercise testing. Eleven healthy subjects, nine patients in New York Heart Association (NYHA) functional class I (IDC-A group) and 11 in NYHA functional class II and III (IDC-B group) performed a symptom-limited, graded bicycle exercise test. Resting, peak and various postexercise levels of plasma free and scCA were determined by high-pressure liquid chromoatography. Resting CA levels obtained in the supine position were remarkable for elevations of free norepinephrine (NE) in IDC-B patients (355 ± 157 ng/l) as compared to IDC-A patients (177 ± 54, p = 0.006) or healthy controls (193 ± 74, p = 0.007). Similarly, scNE was highest in IDC-B patients with 1856 ± 1089 ng/l, followed by IDC-A (1028 ± 187, p = 0.025) and control subjects (1109 ± 440, p = 0.025). There was a highly significant correlation between free and scNE (r = 0.76, p < 0.0005). Whereas resting free dopamine (DA) levels were comparable in all three groups, scDA was found to be elevated clearly in IDC-B patients (8772 ± 2097 ng/l) and significantly different to IDC-A (5786 ± 2481, p = 0.01) or control subjects (4892 ± 1575, p = 0.0005). The NYHA functional class and maximum exercise performance correlated best with resting scDA (r = 0.68, p = 0.001 and r = 0.56, p = 0.005, respectively). At peak exercixe, IDC-B patients exhibited a significant decrease in scNE and sc epinephrine (E) (from 1856 ± 1089 to 1495 ± 932 ng/l, p < 0.005 and from 491 ± 173 to 282 ± 143 ng/l, p < 0.01) compared to controls (from 1109 ± 444 to 1094 ± 548 ng/l and from 379 ± 200 to 329 ± 134 ng/l). In IDC-B patients this decrease in scNE and scE at peak exercise was related inversely to the rise in free NE and E (r = −0.81, p < 0.005 and r = −0.68, p < 0.05). Resting hemodynamic indices generally were reflected better by some free CA rather than by conjugated forms or by parameters of clinical performance. These findings suggest that in addition to free or scNE levels, resting scDA is elevated in symptomatic patients with IDC. In the present study, scDA proved to be a more sensitive marker of heart failure than resting free NE. Maximum exercise performance in symptomatic patients with IDC was accompanied by decreases in scNE and scE that were correlated to the degree of elevation of their respective free fractions.

Harald Dobnig, Orthopedic Research, 3-69 Medical Sciences Building, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA