bluuebunny/crossref_metadata_2025 huggingface dataset:
Plio-Pleistocene Landscape Evolution of the Turano River Basin (Central Apennines, Italy): Insights from Continental Deposits’ Analysis and Drainage Network Development
Authors: Mancinelli Vania , Cavinato Gian Paolo , Ciavattella Francesco , Cecili Alessandro , D’Orefice Maurizio , Epifani Carmela , Esposito Gianluca , Fazzini Massimiliano , Paglia Giorgio , Parotto Maurizio , Miccadei Enrico
Published: June 2021

Quaternary continental deposits record spatio-temporal changes of the landscape and offer insights for drainage network analysis and paleoenvironmental reconstructions. This paper focuses on the Turano River, a left tributary of the Velino River, which flows in the southwestern Abruzzo area at the boundary with Lazio Region. Its basin preserves lithological and morphological field evidence particularly suitable for reconstructing the long-term geomorphological evolution of the Central Apennines and the drainage network development. In detail, the Turano River was investigated through a drainage basin-scale analysis incorporating morphometric analysis, field mapping, continental deposits analysis, and integrated drainage network analysis. This approach allowed us to define a drainage network reversal process, clearly highlighted by the spatial arrangement of continental deposits, spanning from Upper Pliocene to Holocene. The results also indicated tectonic activity as the main factor driving incision and river inversion processes. The work contributes to identifying and describing the main steps of the Quaternary landscape evolution of this mountainous catchment and its morphoneotectonic framework. Therefore, it could represent a methodological tool for multidisciplinary studies in similar mountainous catchments to support any territorial planning activity, from large infrastructure localization (i.e., artificial dams) to sustainable land management.
Lateral Constrained Inversion of DC-Resistivity Data Observed at the Area North of Tenth of Ramadan City, Egypt for Groundwater Exploration
Authors: Genedi Mohamed , Ghazala Hosni , Mohamed Adel , Massoud Usama , Tezkan Bülent
Published: June 2021

In the arid climate area north of Tenth of Ramadan City, southeast of the Nile Delta, Egypt, it is necessary to search for additional water resources for sustainable developments such as agricultural and industrial activities. Thirty two vertical electrical soundings (VES) of a electrical resistivity (DC) survey were carried out along four main profiles by using the Schlumberger array with electrode distances (AB/2) up to 500 m, to explore the shallow Pleistocene groundwater aquifer. The collected data was interpreted by a one-dimensional laterally constrained inversion (1D-LCI) and two-dimensional inversion algorithms to derive a best fit layered-earth resistivity model. The derived resistivity sections are geologically well interpreted based on information taken from the available water boreholes (P2-Well and P3-Well). The lateral constraints are part of the inversion where all data sets are inverted simultaneously, and consequently the output models are balanced between the constraints and the data-model fit. The 1D-LCI offers good analysis of the model parameters, which was successfully used to characterize a zone of groundwater aquifer, as it produces a laterally smooth model with sharp layer boundaries. The 1D-LCI inversion results show that the study area is subdivided into five geo-electrical layers of varied resistivity and thickness. In particular, the resistivity values of the last layer range between 9.3 and 110 Ωm representing the existing shallow Pleistocene aquifer located at depths between 134.5 and 118.4 m. Such results are tied and confirmed well with the results of the 2D inversion of the DC data. It reveals three interpreted geo-electric layers along the four profiles and shows that the area is affected by some normal faults striking nearly in the E–W direction. The very low resistivities of the groundwater aquifer beneath the agricultural part of the survey area probably indicates contamination due to the possible effect of irrigation operated in the cultivated lowlands. The results obtained could help the stakeholder to find additional information about the ground water aquifers in the newly reclaimed arid area and possible locations of new sites for drilling new water wells as additional water resources.
Evidencing the Influence of the COVID-19 Pandemic and Imposed Lockdown Measures on Fitness Status in Adolescents: A Preliminary Report
Authors: Sunda Mirela , Gilic Barbara , Peric Ivan , Jurcev Savicevic Anamarija , Sekulic Damir
Published: June 2021

It was established that the COVID-19 pandemic resulted in decreased physical activity levels, potentially leading to reduced physical fitness. The aim of this research was to investigate the impact of the COVID-19 lockdown on fitness indices in adolescents. We observed high school adolescents (33% girls; aged 15–17 years) divided into two cohorts. The first cohort (control; n = 48) included adolescents who were in school during the 2018/2019 year, while the second cohort (lockdown; n = 66) was observed during the school year 2019/2020 when COVID-19 lockdown measures were imposed. Variables included body height, body mass, BMI, sit-ups, and the 600 m dash. Both cohorts were tested at the beginning and at the end of the observed school years. A factorial analysis of variance for repeated measurements evidenced an improvement in sit-ups and the 600 m dash in the control, and a decrease of the same capacities in the lockdown cohort. In the lockdown cohort, a decrease in muscular fitness was more evident in boys than in girls. No differential changes between cohorts were evidenced in anthropometrics. Results suggest that the COVID-19 lockdown negatively influenced muscular fitness status in adolescents, especially in boys.
Comparing Idiopathic Chronic Fatigue and Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) in Males: Response to Two-Day Cardiopulmonary Exercise Testing Protocol
Authors: van Campen C. (Linda) M. C. , Visser Frans C.
Published: June 2021

(1) Introduction: Multiple studies have shown that peak oxygen consumption is reduced in the majority of myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS )patients, using the gold standard for measuring exercise intolerance: cardiopulmonary exercise testing (CPET). A 2-day CPET protocol has shown different results on day 2 in ME/CFS patients compared to sedentary controls. No comparison is known between ME/CFS and idiopathic chronic fatigue (ICF) for 2-day CPET protocols. We compared ME/CFS patients with patients with chronic fatigue who did not fulfill the ME/CFS criteria in a male population and hypothesized a different pattern of response would be present during the 2nd day CPET. (2) Methods: We compared 25 male patients with ICF who had completed a 2-day CPET protocol to an age-/gender-matched group of 26 male ME/CFS patients. Measures of oxygen consumption (VO2), heart rate (HR), systolic and diastolic blood pressure, workload (Work), and respiratory exchange ratio (RER) were collected at maximal (peak) and ventilatory threshold (VT) intensities. (3) Results: Baseline characteristics for both groups were similar for age, body mass index (BMI), body surface area, (BSA), and disease duration. A significant difference was present in the number of patients with fibromyalgia (seven ME/CFS patients vs. zero ICF patients). Heart rate at rest and the RER did not differ significantly between CPET 1 and CPET 2. All other CPET parameters at the ventilatory threshold and maximum exercise differed significantly (p-value between 0.002 and <0.0001). ME/CFS patients showed a deterioration of performance on CPET2 as reflected by VO2 and workload at peak exercise and ventilatory threshold, whereas ICF patients showed improved performance on CPET2 with no significant change in peak workload. (4) Conclusion: This study confirms that male ME/CFS patients have a reduction in exercise capacity in response to a second-day CPET. These results are similar to published results in male ME/CFS populations. Patients diagnosed with ICF show a different response on day 2, more similar to sedentary and healthy controls.
Book Review: Cohn, S.L. (Ed.). Decision Making in Perioperative Medicine: Clinical Pearls. (New York: McGraw-Hill), 2021. ISBN: 978-1-260-46810-6
Authors: Parrish Richard H.
Published: June 2021

Cohn’s work fills a void in the perioperative care literature by providing a concise, comprehensive, practical, and authoritative guide to the medical management of common periprocedural issues and scenarios [...]
Extending Adjuvant Endocrine Therapy for 10 Years: A Mixed-Methods Analysis of Women’s Decision Making in an Online Breast Cancer Forum
Authors: Eraso Yolanda , Stefler Denes , Moon Zoe , Rossi Leda , Assefa Sidona
Published: June 2021

An additional 5 years of treatment with adjuvant hormonal therapy, to complete 10 years of medication, is recommended to reduce the risk of breast cancer recurrence. Yet professionals and patients should balance this benefit against side effects and toxicities. Little is known about women’s decision making regarding persistence with extended endocrine therapy. In this study, we collected data from a UK online breast cancer forum to analyse patterns of persistence and its associated factors. A mixed-methods exploratory sequential design was used, with a qualitative analysis of text (n = 61 individuals) informing the development of a quantitative instrument to statistically analyse the prevalence of the findings (n = 130). Our findings identified three different groups of women who had to make decisions regarding persistence with treatment: those about to complete 5 years of therapy, those who decided to extend treatment, and those who were initially prescribed 10 years. Factors affecting persistence were, lack of self-efficacy in managing side effects, lack of reassurance about individual risk of recurrence, and impact on quality of life. Interventions such as training of healthcare professionals including risk communication, medication reviews by clinical pharmacists, and re-planning of services in follow-up care, should better support women’s needs in extended hormonal therapy.
Understanding Contextual Factors Effects and Their Implications for Italian Physiotherapists: Findings from a National Cross-Sectional Study
Authors: Bisconti Mattia , Venturin Davide , Bianco Alessandra , Capurso Valentina , Giovannico Giuseppe
Published: June 2021

An online cross-sectional survey was conducted using Google Docs software. The aim was to understand the management of contextual factors and to identify which are most relevant and which clinicians underestimate. A total of 1250 physiotherapists were chosen from the database of the Manual Therapists group mailing list (GTM-IFOMPT MO) from July to August 2020. A total of 699 responses were received that were considered valid (56%). Participants (40.83%) identified contextual factors (CFs) as “any element, even involuntary, with which the patient interacts during treatment”. Physiotherapists individually chose the representation of CF with the “therapeutic relationship” (82.9%), followed by “therapeutic setting” (75.8%). This choice differed between participants belonging to different age groups. Participants favor communication strategies (76.93%). More than half (57.88%) pay attention to patient involvement during the course of care; and in response to the patients’ doubts about the use of treatments with limited scientific efficacy, they suggest different medical treatments. The patient’s previous clinical experience is not considered significant and does not influence the choice of treatment. Subsequently, however, the participants reported that they stimulate the patients’ positive expectations of the success of the clinical outcome (45.27%). Knowledge of contextual factors in physiotherapy appears limited and very heterogeneous. Future research could increase the focus on professional development.
Morphological, Molecular and Genomic Characterization of Two Inter-Subspecific Hybrids between Olive Cultivars and Olive Subspecies
Authors: Li Jinhua , Ji Xinyue , Wang Zhaoshan , Zeng Yanfei , Zhang Jianguo
Published: June 2021

Two inter-subspecific F1 hybrids have been obtained by crossing olive cultivars (‘Frantoio’ and ‘Coratina’) with pollen donors from olive subspecies (Olea europaea subsp. cuspidata) to enrich the germplasm of cultivated olive in southern China. This study aimed to investigate the characterization of morphological traits and molecular markers in the two hybrids and their parents of crosses. The morphological study showed a significant difference between genotypes according to the main discriminative parameters on qualitative and quantitative traits of leaf, fruit, and endocarp. A set of six co-dominant polymorphic simple sequence repeats (SSRs) were used for molecular identification, and SSR analysis confirmed that two progenies were the offspring of their cited parents based on the presence of parental specific SSR alleles. Three single-copy nuclear loci (SCNL) primer pairs were used for amplification of single-copy genes in the two progenies and their parents and after then PCR products were sequenced. Sequence alignment analysis on the effective data showed a total of 15 different base sites between two progenies, which were confirmed as true inter-specific hybrids between olive cultivars and subsp. cuspidata.
Adoption of Tobacco 21: A Cross-Case Analysis of Ten US States
Authors: Hudson Shawna V. , Kurti Marin , Howard Jenna , Sanabria Bianca , Schroth Kevin R. J. , Hrywna Mary , Delnevo Cristine D.
Published: June 2021

Despite the recent push for Tobacco 21 legislation in the US and the national adoption of Tobacco 21, there is a paucity of data on the process of policy adoption. To explore the key factors that served as facilitators or challenges to the passage of state T21 laws that apply to the sale of all tobacco products to anyone under 21 years of age, we conducted a comparative, cross-case study in ten states that adopted Tobacco 21 between 2016 and 2019. Stakeholders from selected states were identified via snowball sampling, and interviews were conducted from November 2018 to March 2020. Three primary factors emerged as facilitators to the passage of state T21 laws: (1) increased attention on e-cigarettes as the product driving an overall increase in youth tobacco use and depiction of an “e-cigarette epidemic”, (2) having at least one influential policy entrepreneur or champion, and (3) traction from other states or local municipalities passing T21 legislation. Challenges to T21′s success included (1) influence of the tobacco industry, (2) the bill’s low ranking among legislative priorities, and (3) controversy among advocates and policymakers over bill language. As e-cigarette rates spiked, T21 bills became legislative priorities, traction from other successful efforts mounted, and ultimately, the tobacco industry flipped from opposing to supporting T21 laws. Despite these favorable headwinds, advocates struggled increasingly to pass bills with ideal policy language.
Pilot Study to Assess the Feasibility of a Mobile Unit for Remote Cognitive Screening of Isolated Elderly in Rural Areas
Authors: Zeghari Radia , Guerchouche Rachid , Tran Duc Minh , Bremond François , Lemoine Maria Pascale , Bultingaire Vincent , Langel Kai , De Groote Zeger , Kuhn Francis , Martin Emmanuelle , Robert Philippe , König Alexandra
Published: June 2021

Background: Given the current COVID-19 pandemic situation, now more than ever, remote solutions for assessing and monitoring individuals with cognitive impairment are urgently needed. Older adults in particular, living in isolated rural areas or so-called ‘medical deserts’, are facing major difficulties in getting access to diagnosis and care. Telemedical approaches to assessments are promising and seem well accepted, reducing the burden of bringing patients to specialized clinics. However, many older adults are not yet adequately equipped to allow for proper implementation of this technology. A potential solution could be a mobile unit in the form of a van, equipped with the telemedical system which comes to the patients’ home. The aim of this proof-of-concept study is to evaluate the feasibility and reliability of such mobile unit settings for remote cognitive testing. Methods and analysis: eight participants (aged between 69 and 86 years old) from the city of Digne-Les-Bains volunteered for this study. A basic neuropsychological assessment, including a short clinical interview, is administered in two conditions, by telemedicine in a mobile clinic (equipped van) at a participants’ home and face to face in a specialized clinic. The administration procedure order is randomized, and the results are compared with each other. Acceptability and user experience are assessed among participants and clinicians in a qualitative and quantitative manner. Measurements of stress indicators were collected for comparison. Results: The analysis revealed no significant differences in test results between the two administration procedures. Participants were, overall, very satisfied with the mobile clinic experience and found the use of the telemedical system relatively easy. Conclusion: A mobile unit equipped with a telemedical service could represent a solution for remote cognitive testing overcoming barriers in rural areas to access specialized diagnosis and care.
Hemodynamic Response to Three Types of Urban Spaces before and after Lockdown during the COVID-19 Pandemic
Authors: Olszewska-Guizzo Agnieszka , Mukoyama Ayako , Naganawa Sho , Dan Ippeita , Husain Syeda Fabeha , Ho Cyrus S. , Ho Roger
Published: June 2021

(1) Background: Prolonged lockdowns with stay-at-home orders have been introduced in many countries since the outbreak of the COVID-19 pandemic. They have caused a drastic change in the everyday lives of people living in urbanized areas, and are considered to contribute to a modified perception of the public space. As research related to the impact of COVID-19 restrictions on mental health and well-being emerges, the associated longitudinal changes of brain hemodynamics in healthy adults remain largely unknown. (2) Methods: this study examined the hemodynamic activation patterns of the prefrontal and occipital cortices of 12 participants (5 male, Mage = 47.80, SDage = 17.79, range 25 to 74, and 7 female, Mage = 39.00, SDage = 18.18, range 21 to 65) passively viewing videos from three urban sites in Singapore (Urban Park, Neighborhood Landscape and City Center) at two different time points—T1, before the COVID-19 pandemic and T2, soon after the lockdown was over. (3) Results: We observed a significant and marginally significant decrease in average oxyhemoglobin (Oxy-Hb) over time for each of the visual conditions. For both green spaces (Urban Park and Neighborhood Landscape), the decrease was in the visual cortex, while for the City Center with no green elements, the marginal decrease was observed in the visual cortex and the frontal eye fields. (4) Conclusions: The results suggest that the COVID-19-related lockdown experienced by urban inhabitants may have contributed to decreased brain hemodynamics, which are further related to a heightened risk of mental health disorders, such as depression or a decline in cognitive functions. Moreover, the busy City Center scenes induced a hemodynamic pattern associated with stress and anxiety, while urban green spaces did not cause such an effect. Urban green scenes can be an important factor to offset the negative neuropsychological impact of busy urban environments post-pandemic.
Predicting Lung Cancer in the United States: A Multiple Model Examination of Public Health Factors
Authors: Kamis Arnold , Cao Rui , He Yifan , Tian Yuan , Wu Chuyue
Published: June 2021

In this research, we take a multivariate, multi-method approach to predicting the incidence of lung cancer in the United States. We obtain public health and ambient emission data from multiple sources in 2000–2013 to model lung cancer in the period 2013–2017. We compare several models using four sources of predictor variables: adult smoking, state, environmental quality index, and ambient emissions. The environmental quality index variables pertain to macro-level domains: air, land, water, socio-demographic, and built environment. The ambient emissions consist of Cyanide compounds, Carbon Monoxide, Carbon Disulfide, Diesel Exhaust, Nitrogen Dioxide, Tropospheric Ozone, Coarse Particulate Matter, Fine Particulate Matter, and Sulfur Dioxide. We compare various models and find that the best regression model has variance explained of 62 percent whereas the best machine learning model has 64 percent variance explained with 10% less error. The most hazardous ambient emissions are Coarse Particulate Matter, Fine Particulate Matter, Sulfur Dioxide, Carbon Monoxide, and Tropospheric Ozone. These ambient emissions could be curtailed to improve air quality, thus reducing the incidence of lung cancer. We interpret and discuss the implications of the model results, including the tradeoff between transparency and accuracy. We also review limitations of and directions for the current models in order to extend and refine them.
Maximizing Acceleration and Change of Direction in Sport: A Case Series to Illustrate How the Force-Velocity Profile Provides Additional Information to That Derived from Linear Sprint Time
Authors: Baena-Raya Andrés , Rodríguez-Pérez Manuel A. , Jiménez-Reyes Pedro , Soriano-Maldonado Alberto
Published: June 2021

Sprint running and change of direction (COD) present similar mechanical demands, involving an acceleration phase in which athletes need to produce and apply substantial horizontal external force. Assessing the mechanical properties underpinning individual sprint acceleration might add relevant information about COD performance in addition to that obtained through sprint time alone. The present technical report uses a case series of three athletes with nearly identical 20 m sprint times but with different mechanical properties and COD performances. This makes it possible to illustrate, for the first time, a potential rationale for why the sprint force-velocity (FV) profile (i.e., theoretical maximal force (F0), velocity (V0), maximal power output (Pmax), ratio of effective horizontal component (RFpeak) and index of force application technique (DRF)) provides key information about COD performance (i.e., further to that derived from simple sprint time), which can be used to individualize training. This technical report provides practitioners with a justification to assess the FV profile in addition to sprint time when the aim is to enhance sprint acceleration and COD performance; practical interpretations and advice on how training interventions could be individualized based on the athletes’ differential sprint mechanical properties are also specified.
Community-Based Exercise and Lifestyle Program Improves Health Outcomes in Older Adults with Type 2 Diabetes
Authors: Kirwan Morwenna , Chiu Christine L. , Hay Mellissa , Laing Thomas
Published: June 2021

Background: The aim of this study was to assess the efficacy of Beat It—a community-based exercise and lifestyle intervention—in improving anthropometric and physical fitness outcomes in older adults with type 2 diabetes mellitus (T2DM). Methods: Australians with T2DM who were aged 60 years or older were included. These individuals were enrolled in Beat It, a twice-weekly supervised group exercise and education program conducted over 8 weeks. Anthropometric measurements and physical fitness parameters were assessed at baseline and completion. Physical fitness measures were then compared to validated criterion standards of fitness levels required by older adults to remain physically independent into later life. Results: A total of 588 individuals were included in the study. At baseline, a substantial proportion of the cohort had physical fitness measures that were below the standard for healthy independent living for their gender and age. Significant improvements in waist circumference and physical fitness were observed post program and resulted in an increase in the number of participants who met the standard for healthy independent living. Conclusions: Participation in Beat It improved important health outcomes in older adults with T2DM. A longer-term follow-up is needed to determine whether these positive changes were maintained beyond the delivery of the program.
Does Workers’ Compensation Status Affect Outcomes after Lumbar Spine Surgery? A Systematic Review and Meta-Analysis
Authors: Russo Fabrizio , De Salvatore Sergio , Ambrosio Luca , Vadalà Gianluca , Fontana Luca , Papalia Rocco , Rantanen Jorma , Iavicoli Sergio , Denaro Vincenzo
Published: June 2021

Low back pain (LBP) is currently the leading cause of disability worldwide and the most common reason for workers’ compensation (WC) claims. Studies have demonstrated that receiving WC is associated with a negative prognosis following treatment for a vast range of health conditions. However, the impact of WC on outcomes after spine surgery is still controversial. The aim of this meta-analysis was to systematically review the literature and analyze the impact of compensation status on outcomes after lumbar spine surgery. A systematic search was performed on Medline, Scopus, CINAHL, EMBASE and CENTRAL databases. The review included studies of patients undergoing lumbar spine surgery in which compensation status was reported. Methodological quality was assessed through ROBINS-I and quality of evidence was estimated using the GRADE rating. A total of 26 studies with a total of 2668 patients were included in the analysis. WC patients had higher post-operative pain and disability, as well as lower satisfaction after surgery when compared to those without WC. Furthermore, WC patients demonstrated to have a delayed return to work. According to our results, compensation status is associated with poor outcomes after lumbar spine surgery. Contextualizing post-operative outcomes in clinical and work-related domains helps understand the multifactorial nature of the phenomenon.
Are Demanding Job Situations Associated with Alcohol-Related Presenteeism? The WIRUS-Screening Study
Authors: Bonsaksen Tore , Thørrisen Mikkel Magnus , Skogen Jens Christoffer , Hesse Morten , Aas Randi Wågø
Published: June 2021

Alcohol-related presenteeism (impaired work performance caused by alcohol use) is an important but under-researched topic. The aim of this study was to explore whether psychosocial work environment factors were associated with alcohol-related presenteeism. A cross sectional study of Norwegian employees (n = 6620) was conducted. Logistic regression analyses were used for estimating associations with alcohol-related presenteeism, which was reported among 473 (7.1%) of the employees. Adjusted by age, gender, education level and managerial level, higher levels of overcommitment to work were associated with alcohol-related presenteeism. Higher age, male gender and higher education were also associated with alcohol-related presenteeism. Occupational health services and employers should especially focus on overcommitted employees when designing workplace health promotion programs. Modifying attitudes towards alcohol-related presenteeism among overcommitted employees may be of importance for safety at work.
Kramer Score, an Evidence of Its Use in Accordance with Indonesian Hyperbilirubinemia Published Guideline
Authors: Sampurna Mahendra Tri Arif , Mapindra Muhammad Pradhika , Mahindra Muhammad Pradhiki , Ratnasari Kinanti Ayu , Rani Siti Annisa Dewi , Handayani Kartika Darma , Angelika Dina , Harianto Agus , Utomo Martono Tri , Etika Risa , Sauer Pieter J. J.
Published: June 2021

Background: In some hospitals in low/middle-income countries, methods to determine the bilirubin level in newborn infants are unavailable and based on a clinical evaluation, namely a clinical score designed by Kramer. In this study, we evaluated if this score can be used to identify those infants that need phototherapy. Method: Infants admitted between November 2018 and June 2019 to three hospitals in Surabaya, Indonesia were included. The jaundice intensity was scored using the Kramer score. Blood was sampled for total serum bilirubin (TSB) measurement. The infants were categorized into Treatment Needed (TN) group when treatment with phototherapy was indicated and the No Treatment Needed (NTN) group when phototherapy was not indicated, based on the Indonesian Guideline for hyperbilirubinemia. Result: A total of 280 infants with a mean birth weight of 2744.6 ± 685.8 g and a gestational age of 37.3 ± 2.3 weeks were included. Twenty-seven of 113 (24%) infants with Kramer score 2 needed phototherapy, compared with 41 of 90 (46%) infants with score 3 and 20 of 28 (71%) of infants with score 4. The percentage of infants that needed phototherapy was higher with decreasing gestational age. Conclusion: The Kramer score is an invalid method to distinguish between those infants needing phototherapy and those infants where this treatment is not indicated.
Determinants of Food Choice and Perceptions of Supermarket-Based Nudging Interventions among Adults with Low Socioeconomic Position: The SUPREME NUDGE Project
Authors: Harbers Marjolein C. , Middel Cédric N.H. , Stuber Josine M. , Beulens Joline W.J. , Rutters Femke , van der Schouw Yvonne T.
Published: June 2021

Nudging has received ample attention in scientific literature as an environmental strategy to promote healthy diets, and may be effective for reaching populations with low socioeconomic position (SEP). Therefore, the objective of this study was to investigate how the determinants of food choice shape the perceptions regarding supermarket-based nudging strategies among adults with low SEP. We conducted semi-structured interviews among fifteen adults with low SEP using a pre-defined topic list and visual examples of nudges. Interviews were recorded and transcribed verbatim and content analysis was used to analyse the data. The results show that food costs, convenience, healthiness, taste, and habits were frequently mentioned as determinants of food choice. However, the relative importance of these determinants seemed to be context-dependent. Interviewees generally had a positive attitude towards nudges, especially when they were aligned with product preferences, information needs, and beliefs about the food environment. Still, some interviewees also expressed distrust towards nudging strategies, suspecting ulterior motives. We conclude that nudging strategies should target foods which align with product preferences and information needs. However, the suspicion of ulterior motives highlights an important concern which should be considered when implementing supermarket-based nudging strategies.
INS Error Estimation Based on an ANFIS and Its Application in Complex and Covert Surroundings
Authors: Duan Yabo , Li Huaizhan , Wu Suqin , Zhang Kefei
Published: June 2021

Inertial navigation is a crucial part of vehicle navigation systems in complex and covert surroundings. To address the low accuracy of vehicle inertial navigation in multifaced and covert surroundings, in this study, we proposed an inertial navigation error estimation based on an adaptive neuro fuzzy inference system (ANFIS) which can quickly and accurately output the position error of a vehicle end-to-end. The new system was tested using both single-sequence and multi-sequence data collected from a vehicle by the KITTI dataset. The results were compared with an inertial navigation system (INS) position solution method, artificial neural networks (ANNs) method, and a long short-term memory (LSTM) method. Test results indicated that the accumulative position errors in single sequence and multi-sequences experiments decreased from 9.83% and 4.14% to 0.45% and 0.61% by using ANFIS, respectively, which were significantly less than those of the other three approaches. This result suggests that the ANFIS can considerably improve the positioning accuracy of inertial navigation, which has significance for vehicle inertial navigation in complex and covert surroundings.
Portraying Citizens’ Occupations and Assessing Urban Occupation Mixture with Mobile Phone Data: A Novel Spatiotemporal Analytical Framework
Authors: Zhang Xiaoming , Gao Feng , Liao Shunyi , Zhou Fan , Cai Guanfang , Li Shaoying
Published: June 2021

Mobile phone data is a typical type of big data with great potential to explore human mobility and individual portrait identification. Previous studies in population classifications with mobile phone data only focused on spatiotemporal mobility patterns and their clusters. In this study, a novel spatiotemporal analytical framework with an integration of spatial mobility patterns and non-spatial behavior, through smart phone APP (applications) usage preference, was proposed to portray citizens’ occupations in Guangzhou center through mobile phone data. An occupation mixture index (OMI) was proposed to assess the spatial patterns of occupation diversity. The results showed that (1) six types of typical urban occupations were identified: financial practitioners, wholesalers and sole traders, IT (information technology) practitioners, express staff, teachers, and medical staff. (2) Tianhe and Yuexiu district accounted for most employed population. Wholesalers and sole traders were found to be highly dependent on location with the most obvious industrial cluster. (3) Two centers of high OMI were identified: Zhujiang New Town CBD and Tianhe Smart City (High-Tech Development Zone). It was noted that CBD has a more profound effect on local as well as nearby OMI, while the scope of influence Tianhe Smart City has on OMI is limited and isolated. This study firstly integrated both spatial mobility and non-spatial behavior into individual portrait identification with mobile phone data, which provides new perspectives and methods for the management and development of smart city in the era of big data.
Ecosystem-Dependent Responses of Vegetation Coverage on the Tibetan Plateau to Climate Factors and Their Lag Periods
Authors: Cai Shuohao , Song Xiaoning , Hu Ronghai , Guo Da
Published: June 2021

The spatiotemporal variation characteristics of the Normalized Difference Vegetation Index (NDVI) and its climate response patterns are of significance in deepening our understanding of regional vegetation and climate change. The response of vegetation to climate factors varies spatially and may have lag periods. In this paper, we studied the spatiotemporal responses of vegetation to climatic factors on an ecosystem-dependent scale using GIMMS NDVI3g data and climatic parameters. Pure pixels with a single vegetation type were firstly extracted to reduce the influence of mixed vegetation types. Then, a lag correlation analysis was used to explore the lag effects of climatic parameters affecting NDVI. Finally, the stepwise regression method was adopted to calculate the regression equation for NDVI and meteorological data with the consideration of effect lag times. The results show that precipitation has significant lag effects on vegetation. Temperature is the main climatic factor that affects most vegetation types at the start of growing season. At the end of growing season, the temperate desert, temperate steppe, and temperate desert steppe are greatly affected by precipitation. Moreover, the alpine steppe, alpine desert, alpine meadow, and alpine sparse vegetation are greatly affected by temperature. The needleleaf forest, subalpine scrub, and broadleaf evergreen forest are sensitive to sunshine percentage during almost the whole growing season. These findings could contribute to a better understanding of the drivers and mechanisms of vegetation degradation on the Tibetan Plateau.
Promoter Methylation of PRKCB, ADAMTS12, and NAALAD2 Is Specific to Prostate Cancer and Predicts Biochemical Disease Recurrence
Authors: Daniunaite Kristina , Bakavicius Arnas , Zukauskaite Kristina , Rauluseviciute Ieva , Lazutka Juozas Rimantas , Ulys Albertas , Jankevicius Feliksas , Jarmalaite Sonata
Published: June 2021

The molecular diversity of prostate cancer (PCa) has been demonstrated by recent genome-wide studies, proposing a significant number of different molecular markers. However, only a few of them have been transferred into clinical practice so far. The present study aimed to identify and validate novel DNA methylation biomarkers for PCa diagnosis and prognosis. Microarray-based methylome data of well-characterized cancerous and noncancerous prostate tissue (NPT) pairs was used for the initial screening. Ten protein-coding genes were selected for validation in a set of 151 PCa, 51 NPT, as well as 17 benign prostatic hyperplasia samples. The Prostate Cancer Dataset (PRAD) of The Cancer Genome Atlas (TCGA) was utilized for independent validation of our findings. Methylation frequencies of ADAMTS12, CCDC181, FILIP1L, NAALAD2, PRKCB, and ZMIZ1 were up to 91% in our study. PCa specific methylation of ADAMTS12, CCDC181, NAALAD2, and PRKCB was demonstrated by qualitative and quantitative means (all p < 0.05). In agreement with PRAD, promoter methylation of these four genes was associated with the transcript down-regulation in the Lithuanian cohort (all p < 0.05). Methylation of ADAMTS12, NAALAD2, and PRKCB was independently predictive for biochemical disease recurrence, while NAALAD2 and PRKCB increased the prognostic power of multivariate models (all p < 0.01). The present study identified methylation of ADAMTS12, NAALAD2, and PRKCB as novel diagnostic and prognostic PCa biomarkers that might guide treatment decisions in clinical practice.
Glycogen Synthase Kinase 3 Beta Regulates the Human Aryl Hydrocarbon Receptor Cellular Content and Activity
Authors: Yang Yujie , Chan William K.
Published: June 2021

The aryl hydrocarbon receptor (AHR) is a cytosolic receptor which is involved in diverse cellular events in humans. The most well-characterized function of AHR is its ability to upregulate gene transcription after exposure to its ligands, such as environmental toxicants, dietary antioxidants, drugs, and endogenous ligands. The cellular content of AHR is partly controlled by its degradation via the ubiquitin–proteasome system and the lysosome-dependent autophagy. We used human cervical cancer (HeLa) cells to investigate how AHR undergoes protein degradation and how its activity is modulated. Since the glycogen synthase kinase 3 beta (GSK3β)-mediated phosphorylation can trigger protein degradation and substrates of GSK3β contain stretches of serine/threonine residues which can be found in AHR, we examined whether degradation and activity of AHR can be controlled by GSK3β. We observed that AHR undergoes the GSK3β-dependent, LC3-mediated lysosomal degradation without ligand treatment. The AHR can be phosphorylated in a GSK3β-dependent manner at three putative sites (S436/S440/S444, S689/S693/T697, and S723/S727/T731), which leads to lysosomal degradation of the AHR protein. Inhibition of the GSK3β activity suppresses the ligand-activated transcription of an AHR target gene in HeLa, human liver cancer (Hep3B), and human breast cancer (MCF-7) cells. Collectively, our findings support that phosphorylation of AHR by GSK3β is essential for the optimal activation of its target gene transcription and this phosphorylation may partake as an “off” switch by subjecting the receptor to lysosomal degradation.
Inhibition of Carbonic Anhydrase IX Promotes Apoptosis through Intracellular pH Level Alterations in Cervical Cancer Cells
Authors: Temiz Ebru , Koyuncu Ismail , Durgun Mustafa , Caglayan Murat , Gonel Ataman , Güler Eray Metin , Kocyigit Abdurrahim , Supuran Claudiu T.
Published: June 2021

Carbonic anhydrase IX (CAIX) is a hypoxia-related protein that plays a role in proliferation in solid tumours. However, how CAIX increases proliferation and metastasis in solid tumours is unclear. The objective of this study was to investigate how a synthetic CAIX inhibitor triggers apoptosis in the HeLa cell line. The intracellular effects of CAIX inhibition were determined with AO/EB, AnnexinV-PI, and γ-H2AX staining; measurements of intracellular pH (pHi), reactive oxygen species (ROS), and mitochondrial membrane potential (MMP); and analyses of cell cycle, apoptotic, and autophagic modulator gene expression (Bax, Bcl-2, caspase-3, caspase-8, caspase-9, caspase-12, Beclin, and LC3), caspase protein level (pro-caspase 3 and cleaved caspase-3, -8, -9), cleaved PARP activation, and CAIX protein level. Sulphonamide CAIX inhibitor E showed the lowest IC50 and the highest selectivity index in CAIX-positive HeLa cells. CAIX inhibition changed the morphology of HeLa cells and increased the ratio of apoptotic cells, dramatically disturbing the homeostasis of intracellular pHi, MMP and ROS levels. All these phenomena consequent to CA IX inhibition triggered apoptosis and autophagy in HeLa cells. Taken together, these results further endorse the previous findings that CAIX inhibitors represent an important therapeutic strategy, which is worth pursuing in different cancer types, considering that presently only one sulphonamide inhibitor, SLC-0111, has arrived in Phase Ib/II clinical trials as an antitumour/antimetastatic drug.
SREBP-1c Deficiency Affects Hippocampal Micromorphometry and Hippocampus-Dependent Memory Ability in Mice
Authors: Ang Mary Jasmin , Lee Sueun , Wada Mai , Weerasinghe-Mudiyanselage Poornima D. E. , Kim Sung-Ho , Shin Taekyun , Jeon Tae-Il , Im Seung-Soon , Moon Changjong
Published: June 2021

Changes in structural and functional neuroplasticity have been implicated in various neurological disorders. Sterol regulatory element-binding protein (SREBP)-1c is a critical regulatory molecule of lipid homeostasis in the brain. Recently, our findings have shown the potential involvement of SREBP-1c deficiency in the alteration of novel modulatory molecules in the hippocampus and occurrence of schizophrenia-like behaviors in mice. However, the possible underlying mechanisms, related to neuronal plasticity in the hippocampus, are yet to be elucidated. In this study, we investigated the hippocampus-dependent memory function and neuronal architecture of hippocampal neurons in SREBP-1c knockout (KO) mice. During the passive avoidance test, SREBP-1c KO mice showed memory impairment. Based on Golgi staining, the dendritic complexity, length, and branch points were significantly decreased in the apical cornu ammonis (CA) 1, CA3, and dentate gyrus (DG) subregions of the hippocampi of SREBP-1c KO mice, compared with those of wild-type (WT) mice. Additionally, significant decreases in the dendritic diameters were detected in the CA3 and DG subregions, and spine density was also significantly decreased in the apical CA3 subregion of the hippocampi of KO mice, compared with that of WT mice. Alterations in the proportions of stubby and thin-shaped dendritic spines were observed in the apical subcompartments of CA1 and CA3 in the hippocampi of KO mice. Furthermore, the corresponding differential decreases in the levels of SREBP-1 expression in the hippocampal subregions (particularly, a significant decrease in the level in the CA3) were detected by immunofluorescence. This study suggests that the contributions of SREBP-1c to the structural plasticity of the mouse hippocampus may have underlain the behavioral alterations. These findings offer insights into the critical role of SREBP-1c in hippocampal functioning in mice.
Extinction vs. Abstinence: A Review of the Molecular and Circuit Consequences of Different Post-Cocaine Experiences
Authors: Schwendt Marek , Knackstedt Lori A.
Published: June 2021

The intravenous cocaine self-administration model is widely used to characterize the neurobiology of cocaine seeking. When studies are aimed at understanding relapse to cocaine-seeking, a post-cocaine abstinence period is imposed, followed by “relapse” tests to assess the ability of drug-related stimuli (“primes”) to evoke the resumption of the instrumental response previously made to obtain cocaine. Here, we review the literature on the impact of post-cocaine abstinence procedures on neurobiology, finding that the prelimbic and infralimbic regions of the prefrontal cortex are recruited by extinction training, and are not part of the relapse circuitry when extinction training does not occur. Pairing cocaine infusions with discrete cues recruits the involvement of the NA, which together with the dorsal striatum, is a key part of the relapse circuit regardless of abstinence procedures. Differences in molecular adaptations in the NA core include increased expression of GluN1 and glutamate receptor signaling partners after extinction training. AMPA receptors and glutamate transporters are similarly affected by abstinence and extinction. Glutamate receptor antagonists show efficacy at reducing relapse following extinction and abstinence, with a modest increase in efficacy of compounds that restore glutamate homeostasis after extinction training. Imaging studies in humans reveal cocaine-induced adaptations that are similar to those produced after extinction training. Thus, while instrumental extinction training does not have face validity, its use does not produce adaptations distinct from human cocaine users.
The Function of the Histamine H4 Receptor in Inflammatory and Inflammation-Associated Diseases of the Gut
Authors: Schirmer Bastian , Neumann Detlef
Published: June 2021

Histamine is a pleiotropic mediator involved in a broad spectrum of (patho)-physiological processes, one of which is the regulation of inflammation. Compounds acting on three out of the four known histamine receptors are approved for clinical use. These approved compounds comprise histamine H1-receptor (H1R) antagonists, which are used to control allergic inflammation, antagonists at H2R, which therapeutically decrease gastric acid release, and an antagonist at H3R, which is indicated to treat narcolepsy. Ligands at H4R are still being tested pre-clinically and in clinical trials of inflammatory diseases, including rheumatoid arthritis, asthma, dermatitis, and psoriasis. These trials, however, documented only moderate beneficial effects of H4R ligands so far. Nevertheless, pre-clinically, H4R still is subject of ongoing research, analyzing various inflammatory, allergic, and autoimmune diseases. During inflammatory reactions in gut tissues, histamine concentrations rise in affected areas, indicating its possible biological effect. Indeed, in histamine-deficient mice experimentally induced inflammation of the gut is reduced in comparison to that in histamine-competent mice. However, antagonists at H1R, H2R, and H3R do not provide an effect on inflammation, supporting the idea that H4R is responsible for the histamine effects. In the present review, we discuss the involvement of histamine and H4R in inflammatory and inflammation-associated diseases of the gut.
RNA Interference by Ingested Dsrna-Expressing Bacteria to Study Porphyrin Pigmentation in Crassostrea gigas
Authors: Hu Biyang , Li Qi , Yu Hong
Published: June 2021

Porphyrins are a widespread group of pigments in nature which are believed to contribute to shell colors in mollusks. Previous studies have provided candidate genes for porphyrin shell coloration, however, the linkage analysis between functional genes and porphyrin pigmentation remains unclear in mollusks. RNA interference is a powerful molecular tool for analyzing the loss of functions of genes in vivo and alter gene expression. In this study, we used unicellular alga Platymonas subcordiformis and Nitzschia closterium f. minutissima as vectors to feed oysters with Escherichia coli strain HT115 engineered to express double-stranded RNAs targeting specific genes involved in porphyrin synthesis. A strain of Crassostrea gigas with orange shell was used to target key haem pathway genes expression using the aforementioned approach. We show here that feeding the oysters with E. coli, containing dsRNA targeting pigmentation genes, can cause changes in the color of the newly deposited shell. For example, the RNAi knockdown of CgALAS and CgPBGD resulted in the loss of uroporphyrin pigmentation from the shell due to the accumulation of the pigment in the oyster’s mantle. The study probed the crucial role of ALAS and PBGD genes potential functions of uroporphyrin production and shell color pigmentation in C. gigas.