bluuebunny/crossref_metadata_2025 huggingface dataset:
Peripheral Nerve Imaging Aids in the Diagnosis of Immune-Mediated Neuropathies—A Case Series
Authors: Dörner Marc , Schreiber Frank , Stephanik Heike , Tempelmann Claus , Winter Natalie , Stahl Jan-Hendrik , Wittlinger Julia , Willikens Sophia , Kramer Magdalena , Heinze Hans-Jochen , Vielhaber Stefan , Schelle Thomas , Grimm Alexander , Schreiber Stefanie
Published: July 2020

Background: Diagnosis of immune-mediated neuropathies and their differentiation from amyotrophic lateral sclerosis (ALS) can be challenging, especially at early disease stages. Accurate diagnosis is, however, important due to the different prognosis and available treatment options. We present one patient with a left-sided dorsal flexor paresis and initial suspicion of ALS and another with multifocal sensory deficits. In both, peripheral nerve imaging was the key for diagnosis. Methods: We performed high-resolution nerve ultrasound (HRUS) and 7T or 3T magnetic resonance neurography (MRN). Results: In both patients, HRUS revealed mild to severe, segmental or inhomogeneous, nerve enlargement at multiple sites, as well as an area increase of isolated fascicles. MRN depicted T2 hyperintense nerves with additional contrast-enhancement. Discussion: Peripheral nerve imaging was compatible with the respective diagnosis of an immune-mediated neuropathy, i.e., multifocal motor neuropathy (MMN) in patient 1 and multifocal acquired demyelinating sensory and motor neuropathy (MADSAM) in patient 2. Peripheral nerve imaging, especially HRUS, should play an important role in the diagnostic work-up for immune-mediated neuropathies and their differentiation from ALS.
Forecasting Bitcoin Trends Using Algorithmic Learning Systems
Authors: Cohen Gil
Published: July 2020

This research has examined the ability of two forecasting methods to forecast Bitcoin’s price trends. The research is based on Bitcoin—USA dollar prices from the beginning of 2012 until the end of March 2020. Such a long period of time that includes volatile periods with strong up and downtrends introduces challenges to any forecasting system. We use particle swarm optimization to find the best forecasting combinations of setups. Results show that Bitcoin’s price changes do not follow the “Random Walk” efficient market hypothesis and that both Darvas Box and Linear Regression techniques can help traders to predict the bitcoin’s price trends. We also find that both methodologies work better predicting an uptrend than a downtrend. The best setup for the Darvas Box strategy is six days of formation. A Darvas box uptrend signal was found efficient predicting four sequential daily returns while a downtrend signal faded after two days on average. The best setup for the Linear Regression model is 42 days with 1 standard deviation.
Patch Antenna-in-Package for 5G Communications with Dual Polarization and High Isolation
Authors: Santos Hugo , Pinho Pedro , Salgado Henrique
Published: July 2020

In this paper, we describe the design of a dual polarized packaged patch antenna for 5G communications with improved isolation and bandwidth for K-band. We introduce a differential feeding technique and a heuristic-based design of a matching network applied to a single layer patch antenna with parasitic elements. This approach resulted in broader bandwidth, reduced layer count, improved isolation and radiation pattern stability. The results were validated through finite element method (FEM) and method of moments (MoM) simulations. A peak gain of 5 dBi, isolation above 40 dB and a radiation efficiency of 60% were obtained.
The Role of Renewable Energies for the Sustainable Energy Governance and Environmental Policies for the Mitigation of Climate Change in Ecuador
Authors: Arroyo M. Flavio R. , Miguel Luis J.
Published: July 2020

This article presents a comparative analysis of energy governance with respect to renewable energy sources in Ecuador. The use of renewable energy sources increases energy security and enables countries to achieve their climate mitigation goals. Ecuador’s energy mix is dominated using fossil fuels and produces only 7.8% of its energy supply from renewable energy. The scenario analysis suggests that using the example of international renewable energy policies will achieve sustainable energy development in Ecuador. Relying less on fossil fuels and decentralizing the electricity sector from the use of thermoelectric plants is the great challenge for the country. Using the enormous water potential that Ecuador has and taking advantage of the sources of solar, biomass and wind energy available in the country will reduce the forecast of 60,233.70 KT CO2 by 2030 that would be reached if current consumption conditions and energy mix are maintained, while designing a long-term energy planning with a greater participation of renewable energies would forecast a CO2 emission of 41,232.30 KT, that is, a reduction of 31.5% in emissions.
Economic Valuation of Green Infrastructure Investments in Urban Renewal: The Case of the Station District in Taichung, Taiwan
Authors: Hsu Kuo-Wei , Chao Jen-Chih
Published: July 2020

The extreme weather conditions that are increasingly affecting Taiwan require urgent solutions, especially as land-use pressures and intensive urban development are triggering new types of vulnerability to natural disasters. Green infrastructure is an especially promising means of enhancing the resilience of urban environments, as well as their residents’ quality of life. However, due to the indirect nature of green investment, the economic value of green infrastructure is not adequately reflected in market prices, and novel methods of economic valuation are needed to ascertain their value. To fulfill that need, this study conducts a cost–benefit analysis of investment in green infrastructure related to urban renewal and identifies economic factors that could directly and indirectly increase environmental quality and promote sustainable development. The main finding of this work is that the increased cost of a green approach for a particular urban-renewal infrastructure project in Taiwan could be recouped in approximately eight years. Specifically, version of the plan based on green infrastructure would cost an additional US $9.2 million up front, but its positive impact would be greater than the non-green version by US $1.2 million per year.
Unmanned Aerial System and Machine Learning Techniques Help to Detect Dead Woody Components in a Tropical Dry Forest
Authors: Campos-Vargas Carlos , Sanchez-Azofeifa Arturo , Laakso Kati , Marzahn Philip
Published: July 2020

Background and Objectives: Increased frequency and intensity of drought events are predicted to occur throughout the world because of climate change. These extreme climate events result in higher tree mortality and fraction of dead woody components, phenomena that are currently being reported worldwide as critical indicators of the impacts of climate change on forest diversity and function. In this paper, we assess the accuracy and processing times of ten machine learning (ML) techniques, applied to multispectral unmanned aerial vehicle (UAV) data to detect dead canopy woody components. Materials and Methods: This work was conducted on five secondary dry forest plots located at the Santa Rosa National Park Environmental Monitoring Super Site, Costa Rica. Results: The coverage of dead woody components at the selected secondary dry forest plots was estimated to range from 4.8% to 16.1%, with no differences between the successional stages. Of the ten ML techniques, the support vector machine with radial kernel (SVMR) and random forests (RF) provided the highest accuracies (0.982 vs. 0.98, respectively). Of these two ML algorithms, the processing time of SVMR was longer than the processing time of RF (8735.64 s vs. 989 s). Conclusions: Our results demonstrate that it is feasible to detect and quantify dead woody components, such as dead stands and fallen trees, using a combination of high-resolution UAV data and ML algorithms. Using this technology, accuracy values higher than 95% were achieved. However, it is important to account for a series of factors, such as the optimization of the tuning parameters of the ML algorithms, the environmental conditions and the time of the UAV data acquisition.
Adaptive Allocation Algorithm for Multi-Radio Multi-Channel Wireless Mesh Networks
Authors: Hassan Walaa , Farag Tamer
Published: July 2020

The wireless mesh network (WMN) has proven to be a great choice for network communication technology. WMNs are composed of access points (APs) that are installed and communicate with each other through multi-hop wireless networks. One or more of these APs acts as a gateway (GW) to the internet. Hosts of WMNs are stationary or mobile. According to the structure of WMNs, some network features may be affected, such as the overall performance, channel interference, and AP connectivity. In this paper, we propose a new adaptive channel allocation algorithm for a multi-radio multi-channel wireless mesh network. The algorithm is aimed to minimize the number of channel reassignments while maximizing the performance under practical constraints. The algorithm defines a decision function for the channel reassignments. The decision function aims to minimize the traffic around the GW. Whenever the traffic changes in the wireless mesh network, the decision function decides which channel radio reassignment should be done. We demonstrated the effectiveness of our algorithm through extensive simulations using Network Simulator 2 (NS-2).
The Role of the CFO of an Industrial Company: An Analysis of the Impact of Blockchain Technology
Authors: Sandner Philipp , Lange Anna , Schulden Philipp
Published: July 2020

This qualitative multiple case study explores the influence of blockchain technology on the chief financial officer (CFO) of an industrial company. Due to the advancing digitalization of business sectors and increasing competitive pressures, industrial companies are forced to promote their own digital transformation to sustain on the market. Here, the literature regards the CFO as a key corporate function to induce digitization initiatives within organizations. The blockchain technology, due to its features of transparency, immutability and cryptography combined with its ability to coordinate data flows of e.g., the Internet of Things (IoT) or Artificial Intelligence (AI), constitutes a suitable instrument for the CFO to meet the requirements of Industry 4.0. This paper provides a contribution to address existing research gaps regarding the application side of blockchain technology. Thus, the objective of this work is to provide corporate financial functions, such as the CFO of an industrial company, with an understanding of the extent to which blockchain technology can be used for the role-specific responsibilities. Therefore, the underlying qualitative study explores the influence of blockchain technology on the CFO-function of an industrial company. Thus, intending to address a research gap on the application side, it asks (1) What is the impact of blockchain technology on the financial as well strategic role of the CFO? (2) What is the impact of blockchain technology in convergence with the Machine Economy on the key performance indicators (KPIs) of the CFO? (3) What is the impact of blockchain-enabled integrated business ecosystems on the role of the CFO? Based on a review of literature, semi-structured expert interviews were conducted with 23 participants. Analysis of the responses demonstrated a considerable impact of blockchain technology on the CFO-function. The results indicate improvements of business processes in regard to efficiency and automation, a relocation of the CFO’s strategic role, improvements of CFO-relevant KPIs through integrating machines into payment networks as well as the emergence of integrated business ecosystems facilitating new forms of inter-organizational collaboration. Necessary prerequisites for adoption include digital competences of the CFO, appropriate organizational structures, digital currencies and identities on the blockchain, a change of the competitive mindset as well as standardized platforms with a neutral governance.
Study on the Influence of Bio-Based Packaging System on Sodium Benzoate Release Kinetics
Authors: Conte Amalia , Lecce Lucia , Iannetti Mariapia , Del Nobile Matteo Alessandro
Published: July 2020

The influence of film structure on the release kinetics of sodium benzoate (SB) from polymeric films is addressed in this study. In particular, four film structures were investigated, two monolayer and two multilayer systems. In particular, in one case, the active substance was uniformly distributed into a chitosan-based matrix, and in the other one, it was previously incorporated into alginate beads before dispersion in the chitosan film, thus realizing two types of monolayer films; on the other hand, the same chitosan film with SB encapsulated in alginate beads was used as the inner layer of a multilayer system constituted by two side films of alginate. The two alginate-based layers were made with two different thicknesses, thus producing a total of two multilayer systems. The release of SB from the above-mentioned films in water was studied by means of a UV/VIS spectrophotometer at 227 nm. A first-order kinetics-type equation was used to quantitatively describe the release data. Results suggest that the film structure strongly affected the release kinetics. In fact, monolayer films showed single-stage release kinetics, whereas the two investigated multilayer systems showed two-stage release kinetics. Further, the presence of alginate beads strongly affected the SB release, thus suggesting the potential of encapsulation to control the release mechanism of active compounds.
Effect of Searing Process on Quality Characteristics and Storage Stability of Sous-Vide Cooked Pork Patties
Authors: Cho Dong Kook , Lee Boin , Oh Hyeonbin , Lee Jae Sang , Kim Young Soon , Choi Young Min
Published: July 2020

This study investigated the effects of searing process before sous-vide (SV) treatment on quality traits, visual attributes, palatability, and storage stability of SV cooked pork patties. Patties were seared on each side by pan-frying for 0 (control), 30 (S30), 60 (S60), 90 (S90), or 120 (S120) s in a stainless-steel pan, and all patties were then vacuum-packed and cooked under thermally controlled conditions at 75 °C for 2 h. Marked differences were observed in quality properties between the control and searing groups, and the S120 group exhibited greater brown surface color and cooking loss compared to the other groups (p < 0.001) due to the additional heating process. Patties from the S60 group showed greater appearance and tenderness acceptability scores compared to patties from the S30 and S120 groups (p < 0.001). On another note, the effects of searing on storage stability were somewhat limited, as they were measured by 2-thiobarbituric acid reactive substance, volatile basic nitrogen, total aerobic bacterial count, and coliforms during 49 d of cold storage. Therefore, searing process before SV treatment can improve the visual attributes and palatability of cooked pork patties, and the optimum searing condition was for 60 s, without impairing the storage stability.
The Efficiency of Atmospheric Dielectric Barrier Discharge Plasma against Escherichia coli and Bacillus cereus on Dried Laver (Porphyra tenera)
Authors: Kim Ji Yoon , Jeon Eun Bi , Choi Man-Seok , Choi Eun Ha , Lim Jun Sup , Choi Jinsung , Park Shin Young
Published: July 2020

This study investigated the effects of atmospheric dielectric barrier discharge (DBD) plasma (1.1 kV, 43 kHz, 5–30 min, N2: 1.5 L/m) on the reduction of Escherichia coli and Bacillus cereus on dried laver. The reductions of E. coli and B. cereus by 5, 10, 20, and 30 min of DBD plasma were 0.56 and 0.24, 0.61 and 0.66, 0.76 and 1.24, and 1.02 and 1.38 log CFU/g, respectively. The D-value of E. coli and B. cereus was predicted as 29.80 and 20.53 min, respectively, using the Weibull model for E. coli (R2 = 0.95) and first-order kinetics for B. cereus (R2 = 0.94). After DBD plasma 5–30 min treatment, there was no change in pH (6.20–6.21) and this value was higher than the untreated dried laver (6.08). All sensory scores in DBD plasma-treated laver were determined as >6 points. The 30 min of DBD plasma is regarded as a novel intervention for the control of potential hazardous bacteria in dried laver.
Bioactive Compounds and Quality of Extra Virgin Olive Oil
Authors: Jimenez-Lopez Cecilia , Carpena Maria , Lourenço-Lopes Catarina , Gallardo-Gomez Maria , Lorenzo Jose M. , Barba Francisco J. , Prieto Miguel A. , Simal-Gandara Jesus
Published: July 2020

(1) Background: Extra virgin olive oil (EVOO) is responsible for a large part of many health benefits associated to Mediterranean diet as it is a fundamental ingredient of this diet. The peculiarities of this golden, highly valued product are in part due to the requirements that must be met to achieve this title, namely, it has to be obtained using exclusively mechanical procedures, its free acidity cannot be greater than 0.8%, it must not show sensory defects, and it has to possess a fruity taste. (2) Methods: All these characteristics are key factors to EVOO quality, thus the chemical composition of these many health-promoting compounds, such as unsaturated fatty acids (which are also the major compounds, especially oleic acid), as well as minor components such as tocopherols or phenolic compounds (which behave as natural antioxidants) must be preserved. (3) Results: Due to the presence of all these compounds, the daily consumption of EVOO entails health benefits such as cardioprotective, antioxidant, anti-inflammatory, anti-tumor properties or acting as regulator of the intestinal microbiota, among others. (4) Conclusions: Taking all together, conserving EVOO chemical composition is essential to preserve its properties, so it is worth to control certain factors during storage like exposure to light, temperature, oxygen presence or the chosen packaging material, to maintain its quality and extend its shelf-life until its consumption.
Effects of Olive Leaf Extracts as Natural Preservative on Retailed Poultry Meat Quality
Authors: Saleh Ebeed , Morshdy Alaa Eldin , El-Manakhly Eman , Al-Rashed Sarah , F. Hetta Helal , Jeandet Philippe , Yahia Ramadan , El-Saber Batiha Gaber , Ali Eman
Published: July 2020

Poultry meat is commonly marketed at refrigerated temperatures (2–5 °C). The major concern for retailers and consumers is the quality and safety of refrigerated poultry meat. During the chilling period, poultry meat undergoes too many undesirable changes due to microbial growth that leads to spoilage and economic loss. Therefore, this study was conducted to assess the effects of olive leaf extracts (OLE) used at three concentrations (0.25, 0.5, and 1%) on the sensory attributes, as well as the chemical and microbiological quality of raw poultry meat stored at 4 ± 1 °C for 15 days. The results revealed that the OLE addition reduced microbial growth successfully, and maintained the chemical quality and sensory attributes of poultry meat. Moreover, OLE extended the shelf-life of the poultry meat that held under proper refrigeration conditions up to 15 days compared to the control group, that was completely spoiled by the sixth day of storage. This study concludes that OLE could be used both as a natural antioxidant and an antimicrobial preservative for chilled poultry meat held at refrigerated temperature.
An Absorption and Plasma Kinetics Study of Monoterpenes Present in Mastiha Oil in Humans
Authors: Papada Efstathia , Gioxari Aristea , Amerikanou Charalampia , Galanis Nikolaos , Kaliora Andriana C.
Published: July 2020

Monoterpenes are bioactive compounds, however studies on their metabolic fate in humans are scarce. The present work aimed to identify and quantify the bioactive monoterpenes myrcene, α- and β-pinene of the Mediterranean product Mastiha Oil, in human plasma after acute consumption of a single dose. This was an open-label, single-arm acute study. After overnight fasting, healthy males were administered with Mastiha Oil. Blood samples were collected on different time-points before and after consumption. A novel GC-MS-MS application was performed to detect and quantify terpenes in MO and in plasma. Serum lipid resistance to oxidation was also determined. Alpha-Pinene, β-pinene and myrcene were identified and quantified in plasma. Alpha-pinene concentration significantly increased after 0.5 h of Mastiha Oil consumption, remaining significantly increased at 1 h, 2 h, 4 h, 6 h and 24 h. Beta-pinene and myrcene followed similar patterns. The increase in serum lipid resistance to oxidation was significant at 1 h, reached its peak at 2 h and remained significant until 4 h. Conclusively, α-pinene, β-pinene and myrcene that are present in Mastiha Oil are absorbed by humans. (ClinicalTrials.gov Identifier: NCT04290312).
GPR174 and ITM2A Gene Polymorphisms rs3827440 and rs5912838 on the X chromosome in Korean Children with Autoimmune Thyroid Disease
Authors: Cho Won Kyoung , Shin Hye-Ri , Lee Na Yeong , Kim Seul Ki , Ahn Moon Bae , Baek In-Cheol , Kim Tai-Gyu , Suh Byung-Kyu
Published: July 2020

(1) Background: Autoimmune thyroid diseases (AITDs) are female predominant and much attention has been focused on G protein-coupled receptor 174 (GPR174) and integral membrane protein 2A (ITM2A) on the X chromosome as Grave’s disease (GD) susceptible locus. (2) Methods: We genotyped four single nucleotide polymorphisms (SNPs), rs3810712, rs3810711, rs3827440, and rs5912838, of GPR174 and ITM2A in 115 Korean children with AITD (M = 25 and F = 90; GD = 74 (14.7 ± 3.6 years), HD = 41 (13.4 ± 3.2 years); GD-thyroid-associated ophthalmopathy (TAO) = 40, GD-non-TAO=34) and 204 healthy Korean individuals (M = 104 and F = 100). The data were analyzed by sex-stratified or combined. (3) Results: Three SNPs, rs3810712, rs3810711 and rs3827440, were found to be in perfect linkage disequilibrium (D’ = 1, r2 = 1). In AITD, HD, GD, GD-TAO, and GD-non-TAO patients, rs3827440 TT/T and rs5912838 AA/A were susceptible and rs3827440 CC/C and rs5912838 CC/C were protective genotypes. When analyzed by sex, rs3827440 TT and rs5912838 AA were susceptible and rs3827440 CC and rs5912838 CC were protective genotypes in female AITD, GD, GD-TAO, and GD-non-TAO subjects. In male AITD patients, rs3827440 T and rs5912838 A were susceptible and rs3827440 C and rs5912838 C were protective genotypes. (4) Conclusions: Polymorphisms in GPR174 and ITM2A genes on the X chromosome might be associated with AITD in Korean children.
A Reappraisal of the Evolutionary and Developmental Pathway of Apomixis and Its Genetic Control in Angiosperms
Authors: Barcaccia Gianni , Palumbo Fabio , Sgorbati Sergio , Albertini Emidio , Pupilli Fulvio
Published: July 2020

Apomixis sensu stricto (agamospermy) is asexual reproduction by seed. In angiosperms it represents an easy byway of life cycle renewal through gamete-like cells that give rise to maternal embryos without ploidy reduction (meiosis) and ploidy restitution (syngamy). The origin of apomixis still represents an unsolved problem, as it may be either evolved from sex or the other way around. This review deals with a reappraisal of the origin of apomixis in order to deepen knowledge on such asexual mode of reproduction which seems mainly lacking in the most basal angiosperm orders (i.e., Amborellales, Nymphaeales and Austrobaileyales, also known as ANA-grade), while it clearly occurs in different forms and variants in many unrelated families of monocots and eudicots. Overall findings strengthen the hypothesis that apomixis as a whole may have evolved multiple times in angiosperm evolution following different developmental pathways deviating to different extents from sexuality. Recent developments on the genetic control of apomixis in model species are also presented and adequately discussed in order to shed additional light on the antagonist theories of gain- and loss-of-function over sexuality.
Standardized Genetic Transformation Protocol for Chrysanthemum cv. ‘Jinba’ with TERMINAL FLOWER 1 Homolog CmTFL1a
Authors: Haider Saba , Gao Yaohui , Gao Yike
Published: July 2020

Chrysanthemum (Chrysanthemum x morifolium Ramat.) cultivar Jinba is a distinctive short-day chrysanthemum that can be exploited as a model organism for studying the molecular mechanism of flowering. The commercial value of Jinba can be increased in global flower markets by developing its proper regeneration and genetic transformation system. By addressing typical problems associated with Agrobacterium-mediated transformation in chrysanthemum, that is, low transformation efficiency and high cultivar specificity, we designed an efficient, stable transformation system. Here, we identify the features that significantly affect the genetic transformation of Jinba and standardize its transformation protocol by using CmTFL1a as a transgene. The appropriate concentrations of various antibiotics (kanamycin, meropenem and carbenicillin) and growth regulators (6-BA, 2,4-D and NAA) for the genetic transformation were determined to check their effects on in vitro plant regeneration from leaf segments of Jinba; thus, the transformation protocol was standardized through Agrobacterium tumefaciens (EHA105). In addition, the presence of the transgene and its stable expression in CmTFL1a transgenic plants were confirmed by polymerase chain reaction (PCR) analysis. The CmTFL1a transgene constitutively expressed in the transgenic plants was highly expressed in shoot apices as compared to stem and leaves. Overexpression of CmTFL1a led to a delay in transition to the reproductive phase and significantly affected plant morphology. This study will help to understand the biological phenomenon of TFL1 homolog in chrysanthemum. Moreover, our findings can explore innovative possibilities for genetic engineering and breeding of other chrysanthemum cultivars.
miR-101b Regulates Lipid Deposition and Metabolism of Primary Hepatocytes in Teleost Yellow Catfish Pelteobagrus fulvidraco
Authors: Chen Guang-Hui , Zhao Tao , Wei Xiao-Lei , Zhang Dian-Guang , Zhuo Mei-Qin , Luo Zhi
Published: July 2020

Excessive fat deposition in the hepatocytes, associated with excess dietary fat intake, was related to the occurrence of fatty livers in fish. miR-101b plays the important roles in controlling lipid metabolism, but the underlying mechanism at the post-transcriptional level remains unclear. The purpose of this study is to explore the roles and mechanism of miR-101b-mediating lipid deposition and metabolism in yellow catfish Pelteobagrus fulvidraco. We found that miR-101b directly targeted fatty acid translocase (cd36), caspase9 (casp9) and autophagy-related gene 4A (atg4a). Furthermore, using palmitic acid (PA) or oleic acid (OA) to incubate the primary hepatocytes of yellow catfish, we demonstrated that miR-101b inversely regulated cd36, casp9, and atg4a expression at the transcriptional level; the inhibition of miR-101b aggravated fatty acids (FAs, PA or OA)-induced lipid accumulation, indicating that miR-101b mediated FAs-induced variations of lipid metabolism in yellow catfish. Taken together, our study gave novel insight into the regulatory mechanism of lipid deposition and metabolism and might provide potential targets for the prevention and treatment of fatty livers in fish.
Extensive Genetic Connectivity and Historical Persistence Are Features of Two Widespread Tree Species in the Ancient Pilbara Region of Western Australia
Authors: Nistelberger Heidi M. , Binks Rachel M. , van Leeuwen Stephen , Coates David J. , McArthur Shelley L. , Macdonald Bronwyn M. , Hankinson Margaret , Byrne Margaret
Published: July 2020

Phylogeographic studies can be used as a tool to understand the evolutionary history of a landscape, including the major drivers of species distributions and diversity. Extensive research has been conducted on phylogeographic patterns of species found in northern hemisphere landscapes that were affected by glaciations, yet the body of literature for older, unaffected landscapes is still underrepresented. The Pilbara region of north-western Australia is an ancient and vast landscape that is topographically complex, consisting of plateaus, gorges, valleys, and ranges, and experiences extreme meteorological phenomena including seasonal cyclonic activity. These features are expected to influence patterns of genetic structuring throughout the landscape either by promoting or restricting the movement of pollen and seed. Whilst a growing body of literature exists for the fauna endemic to this region, less is known about the forces shaping the evolution of plant taxa. In this study we investigate the phylogeography of two iconic Pilbara tree species, the Hamersley Bloodwood (Corymbia hamersleyana) and Western Gidgee (Acacia pruinocarpa), by assessing patterns of variation and structure in several chloroplast DNA regions and nuclear microsatellite loci developed for each species. Gene flow was found to be extensive in both taxa and there was evidence of long-distance seed dispersal across the region (pollen to seed ratios of 6.67 and 2.96 for C. hamersleyana and A. pruinocarpa, respectively), which may result from flooding and strong wind gusts associated with extreme cyclonic activity. Both species possessed high levels of cpDNA genetic diversity in comparison to those from formerly glaciated landscapes (C. hamersleyana = 14 haplotypes, A. pruinocarpa = 37 haplotypes) and showed evidence of deep lineage diversification occurring from the late Miocene, a time of intensifying aridity in this landscape that appears to be a critical driver of evolution in Pilbara taxa. In contrast to another study, we did not find evidence for topographic features acting as refugia for the widely sampled C. hamersleyana.
Drought-Induced Regulatory Cascades and Their Effects on the Nutritional Quality of Developing Potato Tubers
Authors: Da Ros Letitia , Elferjani Raed , Soolanayakanahally Raju , Kagale Sateesh , Pahari Shankar , Kulkarni Manoj , Wahab Jazeem , Bizimungu Benoit
Published: July 2020

Competition for scarce water resources and the continued effects of global warming exacerbate current constraints on potato crop production. While plants’ response to drought in above-ground tissues has been well documented, the regulatory cascades and subsequent nutritive changes in developing tubers have been largely unexplored. Using the commercial Canadian cultivar “Vigor”, plants were subjected to a gradual drought treatment under high tunnels causing a 4 °C increase in the canopy temperature. Tubers were sampled for RNAseq and metabolite analysis. Approximately 2600 genes and 3898 transcripts were differentially expressed by at least 4-fold in drought-stressed potato tubers, with 75% and 69% being down-regulated, respectively. A further 229 small RNAs were implicated in gene regulation during drought. Expression of several small RNA clusters negatively correlated with expression of their six target patatin genes, suggesting involvement in the regulation of storage proteins during drought. The comparison of protein homologues between Solanum tuberosum L. and Arabidopsis thaliana L. indicated that down-regulated genes were associated with phenylpropanoid and carotenoid biosynthesis. As is indicative of reduced flow through the phenylpropanoid pathway, phenylalanine accumulated in drought-stressed tubers. This suggests that there may be nutritive implications to drought stress occurring during the potato tuber bulking phase in sensitive cultivars.
Factors Related to Diabetes Mellitus in the Middle-Aged and Over in Taiwan
Authors: Liang Chia-Chun , Hsu Wei-Chung , Tsai Yao-Te , Weng Shao-Jen , Liu Shih-Chia , Lin Cheng-Hsiang
Published: July 2020

Study Objective: to investigate the factors related to diabetes mellitus in the middle-aged and over in Taiwan. Method: data from seven surveys (in 1989–2011) from the “Taiwan Longitudinal Study on Aging” (TLSA), among cohort B (above the age 60 in 1989), cohort A (aged 50–66 in 1996), and cohort C (aged 50–66 in 2003), were analyzed by the interval-censored Cox model. Results: in the early aging stage (aged 60–64), diabetes mellitus prevalence among the same age appeared the lowest in cohort B, followed by cohort A; cohort C reveals the highest than the young generation. Moreover, suffering from hypertension and kidney diseases are closely related to diabetes mellitus, with the diabetes mellitus suffering hazard ratio of 2.53 (95%: 2.35, 2.73) and 1.26 (95%: 1.11, 1.44) times, respectively. For people with fair and poor self-rated health, the risk of suffering from diabetes mellitus is 1.16 (95%: 1.07, 1.27) and 1.50 (95%: 1.35, 1.67) times compared to people with good self-rated health, respectively. Conclusions: in this study, it is considered that an advanced interval censoring model analysis could more accurately grasp the characteristics of factors in people who are middle-aged and over suffering from diabetes mellitus in Taiwan.
Low-Intensity Exercise Training Additionally Increases Mitochondrial Dynamics Caused by High-Fat Diet (HFD) but Has No Additional Effect on Mitochondrial Biogenesis in Fast-Twitch Muscle by HFD
Authors: Kang Yun Seok , Seong Donghun , Kim Jae Cheol , Kim Sang Hyun
Published: July 2020

This study examines how the high-fat diet (HFD) affects mitochondrial dynamics and biogenesis, and also whether combining it with low-intensity endurance exercise adds to these effects. Six 8-week-old male Sprague–Dawley (SD) rats were put on control (CON; standard chow diet), HF (HFD intake), and HFEx (HFD + low-intensity treadmill exercise) for 6 weeks. As a result, no change in body weight was observed among the groups. However, epididymal fat mass increased significantly in the two groups that had been given HFD. Blood free fatty acid (FFA) also increased significantly in the HF group. While HFD increased insulin resistance (IR), this was improved significantly in the HFEx group. HFD also significantly increased mitochondrial biogenesis-related factors (PPARδ, PGC-1α, and mtTFA) and mitochondrial electron transport chain proteins; however, no additional effect from exercise was observed. Mitochondrial dynamic-related factors were also affected: Mfn2 increased significantly in the HFEx group, while Drp1 and Fis-1 increased significantly in both the HF and HFEx groups. The number of mitochondria in the subsarcolemmal region, and their size in the subsarcolemmal and intermyofibrillar regions, also increased significantly in the HFEx group. Taken overall, these results show that HFD in combination with low-intensity endurance exercise has no additive effect on mitochondrial biogenesis, although it does have such an effect on mitochondrial dynamics by improving IR.
HRV-Guided Training for Professional Endurance Athletes: A Protocol for a Cluster-Randomized Controlled Trial
Authors: Carrasco-Poyatos María , González-Quílez Alberto , Martínez-González-Moro Ignacio , Granero-Gallegos Antonio
Published: July 2020

Physiological training responses depend on sympathetic (SNS) and parasympathetic nervous system (PNS) balance. This activity can be measured using heart rate variability (HRV). Such a measurement method can favor individualized training planning to improve athletes’ performance. Recently, HRV-guided training has been implemented both on professional and amateur sportsmen and sportswomen with varied results. There is a dearth of studies involving professional endurance athletes following a defined HRV-guided training protocol. The objectives of the proposed protocol are: (i) to determine changes in the performance of high-level athletes after following an HRV-guided or a traditional training period and (ii) to determine differences in the athletes’ performance after following both training protocols. This will be a 12-week cluster-randomized controlled protocol in which professional athletes will be assigned to an HRV-based training group (HRV-G) or a traditional-based training group (TRAD-G). TRAD-G will train according to a predefined training program. HRV-G training will depend on the athletes’ daily HRV. The maximal oxygen uptake (VO2max) attained in an incremental treadmill test will be considered as the primary outcome. It is expected that this HRV-guided training protocol will improve functional performance in the high-level athletes, achieving better results than a traditional training method, and thus providing a good strategy for coaches of high-level athletes.
Cultural Influences on Complementary Feeding Beliefs amongst New Chinese Immigrant Mothers in England: A Mixed Methods Study
Authors: Zhang Xiaoning , Rosenthal Diana Margot , Benton Lorna , Lakhanpaul Monica
Published: July 2020

Adequate nutrition during infancy and early childhood is essential for ensuring the growth, health, and development of children so that they can reach their full potential. There is a current void of data on infant and young child feeding practices (IYCF) in ethnic minority communities in the UK; specifically, it is difficult to find accurate Chinese IYCF data in the UK because survey data often includes Chinese in the category of ‘Chinese or other ethnic group’, further contributing to health inequalities. This mixed methods study aimed to explore the cultural influences on IYCF beliefs among new Chinese immigrant mothers. A total of 31 mothers of infants aged 6–23 months were recruited from informal community organizations. All 31 mothers were born in Mainland China, the mean length of their stay after immigrating to the UK was 10 years (range = 1–21 years), and their mean age was 29 ± 3.40 years. When using the Infant Feeding Style Questionnaire (IFSQ) to investigate IYCF beliefs, the highest score was obtained for responsive attention, with a value of 4.28 ± 0.92, indicating that parents were very attentive to child hunger and satiety cues; lower scores were obtained for indulgence soothing (1.82 ± 1.01), indulgence coaxing (2.11 ± 1.18), indulgence pampering (1.90 ± 0.95), and pressuring to soothe (1.92 ± 0.86), indicating lesser maternal indulgence and pressuring/controlling beliefs. A sub-sample (n = 14) participated in semi-structured interviews in order to understand the balancing sources of information and cultural preferences, the influence of traditional Chinese medicine, and language difficulties in accessing health services. The mothers reported barriers of IYCF beliefs and the introduction of solid foods earlier than the NHS guidelines. This study can promote optimal IYCF in Chinese immigrants and show health services the need to reconcile differences between the perceptions of British and Chinese health beliefs.
A Cross-Sectional Study on Benzene Exposure in Pediatric Age and Parental Smoking Habits at Home
Authors: Antonucci Arianna , Vitali Matteo , Martellucci Stefano , Mattei Vincenzo , Protano Carmela
Published: July 2020

After the introduction of the smoke-free legislation, household smoking has become the major source of environmental tobacco smoke (ETS) exposure for children. In our previous research, we found a strong association between urinary unmodified benzene (u-UB) levels and passive smoking exposure related to the home smoking policies (HSP). The aim of the study is to further investigate the impacts of several factors on ETS-exposure in childhood by using u-UB as tobacco-related carcinogen biomarker of exposure. Two cross-sectional studies were performed on the same target population of our previous research, in summer and winter season of the years 2017 and 2018, respectively. A questionnaire and a head space–solid phase micro-extraction/gas chromatography-mass spectrometry (HS-SPME/GC-MS) analytical method were used as investigative procedures. The improvement found in smoking habits, when compared to our previous surveys, reduced the levels of u-UB in children. However, significant differences related to the high number of smokers and smoked cigarettes, in total and at home, still persist. These differences are more relevant in the winter season. Finally, the only effective way for making homes completely smokefree is to develop public health policies for encouraging people to quit or drastically reduce smoking.
Decreased Blood Glucose and Lactate: Is a Useful Indicator of Recovery Ability in Athletes?
Authors: Yang Woo-Hwi , Park Hyuntae , Grau Marijke , Heine Oliver
Published: July 2020

During low-intensity exercise stages of the lactate threshold test, blood lactate concentrations gradually diminish due to the predominant utilization of total fat oxidation. However, it is unclear why blood glucose is also reduced in well-trained athletes who also exhibit decreased lactate concentrations. This review focuses on decreased glucose and lactate concentrations at low-exercise intensity performed in well-trained athletes. During low-intensity exercise, the accrued resting lactate may predominantly be transported via blood from the muscle cell to the liver/kidney. Accordingly, there is increased hepatic blood flow with relatively more hepatic glucose output than skeletal muscle glucose output. Hepatic lactate uptake and lactate output of skeletal muscle during recovery time remained similar which may support a predominant Cori cycle (re-synthesis). However, this pathway may be insufficient to produce the necessary glucose level because of the low concentration of lactate and the large energy source from fat. Furthermore, fatty acid oxidation activates key enzymes and hormonal responses of gluconeogenesis while glycolysis-related enzymes such as pyruvate dehydrogenase are allosterically inhibited. Decreased blood lactate and glucose in low-intensity exercise stages may be an indicator of recovery ability in well-trained athletes. Athletes of intermittent sports may need this recovery ability to successfully perform during competition.
Echocardiography and Newer Imaging Techniques in Diagnosis and Long-Term Follow-Up of Primary Heart Tumors in Children
Authors: Morka Aleksandra , Kohut Joanna , Radzymińska-Chruściel Beata , Mroczek Tomasz , Gładki Marcin , Weryński Piotr , Rudziński Andrzej , Skalski Janusz , Szydłowski Lesław
Published: July 2020

Background: Primary heart tumors (PHTs) in the pediatric population are very rare and do not manifest any characteristic symptoms. Methods: A retrospective analysis of 61 cases was undertaken. Data from three centers for the years 2003–2018 were gathered. The tumors’ clinical course, location, number, hemodynamic, treatment, and follow-up were evaluated. Echocardiography was complemented with magnetic resonance imaging, computer tomography, and histopathological examination. Results: Out of 61 PHT diagnoses, 56 (91.8%) were circumstantial including all 16 (26.2%) prenatal tumors. The reasons for cardiological consultations were arrhythmia, syncopes, lowered physical performance, and murmurs. Only five patients (8.2%) were suspected of tumors based on previous symptoms of sclerosis tuberosa. Rhabdomyoma was the most frequently found PHT (60.7%). The tumors were predominantly located in the ventricles (49.1%) and intraventricular septum (14.9%) and tended to be single (70.5%). About 37.7% of patients suffered from coexistent multi-organ problems, two (3.28%) from congenital heart defects and one (1.64%) from Carney’s syndrome. Tumor resection was performed on 26 (42.7%) patients, of which 16 (61.5%) underwent total and 10 (38.5%) partial tumor resection. During the follow-up (mean 4.3 years), 54 patients (88.5%) have improved or were stable, while seven (11.5%) died. Conclusions: Primary pediatric heart tumors are diagnosed completely circumstantially, and the most common is rhabdomyoma, although arrhythmia may suggest fibroma. Diagnosis of a heart tumor in children is not synonymous with fatal prognosis, and most of them require only constant observation. Life-saving operation allows improvement, while the prognosis for malignant tumors in children is definitely unfavorable.
The Relationships between a Dietary Pattern Linked to Cardiometabolic Risk Factors and Life Satisfaction in Early Adolescence
Authors: Appannah Geeta , Emi Nor Aishah , Gan Wan Ying , Mohd Shariff Zalilah , Shamsuddin Nurainul Hana , Anuar Zaini Azriyanti , Appukutty Mahenderan
Published: July 2020

Little is known about the contribution of dietary patterns of poor quality on life satisfaction among Malaysian children. We evaluated associations between an empirically derived ”high sugar, high fibre, high dietary energy dense (DED) and low fat” dietary pattern and life satisfaction score in adolescents. A total of 548 adolescents aged 13 years were recruited from randomly selected public schools located in three southern states of Peninsular Malaysia. Dietary intake was assessed using a validated food frequency questionnaire (FFQ) while life satisfaction was measured using a Multidimensional Students’ Life Satisfaction Scale (MSLSS). Z-score for a ”high sugar, high fibre, high DED and low fat” dietary pattern was estimated by applying reduced rank regression analysis. Relationships between the dietary pattern and life satisfaction scores were assessed using regression models. Mean and SD of life satisfaction score was higher in girls (70.5 (12.8)) compared to boys (67.6 (15.4)), p < 0.05. The overall life satisfaction score (β = −0.119; 95% CI: −0.125, −0.004) was inversely associated with dietary pattern z-score as well as scores for self (β = −0.13; 95% CI: −0.170, −0.015) and living environment (β = −0.12; 95% CI: −0.163, −0.007) domains in girls. An opposite trend was observed for school domain in boys whereby an increasing dietary pattern score was positively associated with increasing life satisfaction score (β = 0.216; 95% CI: 0.054, 0.36). The finding of this study highlights the role of free sugar and DED particularly, within the framework of whole diet, and target population at risk to improve life satisfaction among adolescents.
Coping Dynamics of Consulting Psychology Doctoral Students Transitioning a Professional Role Identity: A Systems Psychodynamic Perspective
Authors: Barnard Antoni , Flotman Aden-Paul
Published: July 2020

To remain relevant and valuable, the psychology profession in South Africa continues to transform and evolve in response to the changing needs of society. Some psychologists embark on development opportunities to advance their professional qualifications and skills. In doing so, they experience identity tensions inherent to professional identity development and transformation. Understanding how psychologists cope with professional identity transition will enable them to develop a self-efficacious service offering and broaden the reach of psychology in the South African context. The aim of this study was to explore the identity work of a group of eight consulting psychology doctoral students to develop a system psychodynamic understanding of their coping dynamics while transitioning to a professional role identity. Students’ self-reflective essays about becoming a consulting psychologist constituted the data protocols for the study and were analysed through hermeneutic phenomenological analysis. Findings describe how students cope with performance and survival anxieties through anti-task behaviour and immature as well as sophisticated psychodynamic defences. The study contributes to the exploration of the coping concept and its manifestation, by proposing defensive coping as a natural dynamic phenomenon in the process of adapting to a transforming professional identity.