bluuebunny/crossref_metadata_2025 huggingface dataset:
Secreted Cytokines within the Urine of AKI Patients Modulate TP53 and SIRT1 Levels in a Human Podocyte Cell Model
Authors: Erichsen Lars , Thimm Chantelle , Wruck Wasco , Kaierle Daniela , Schless Manon , Huthmann Laura , Dimski Thomas , Kindgen-Milles Detlef , Brandenburger Timo , Adjaye James
Published: May 2023

Acute kidney injury (AKI) is a major kidney disease with a poor clinical outcome. It is a common complication, with an incidence of 10–15% of patients admitted to hospital. This rate even increases for patients who are admitted to the intensive care unit, with an incidence of >50%. AKI is characterized by a rapid increase in serum creatinine, decrease in urine output, or both. The associated symptoms include feeling sick or being sick, diarrhoea, dehydration, decreased urine output (although occasionally the urine output remains normal), fluid retention causing swelling in the legs or ankles, shortness of breath, fatigue and nausea. However, sometimes acute kidney injury causes no signs or symptoms and is detected by lab tests. Therefore, the identification of cytokines for the early detection and diagnosis of AKI is highly desirable, as their application might enable the prevention of the progression from AKI to chronic kidney disease (CKD). In this study, we analysed the secretome of the urine of an AKI patient cohort by employing a kidney-biomarker cytokine assay. Based on these results, we suggest ADIPOQ, EGF and SERPIN3A as potential cytokines that might be able to detect AKI as early as 24 h post-surgery. For the later stages, as common cytokines for the detection of AKI in both male and female patients, we suggest VEGF, SERPIN3A, TNFSF12, ANPEP, CXCL1, REN, CLU and PLAU. These cytokines in combination might present a robust strategy for identifying the development of AKI as early as 24 h or 72 h post-surgery. Furthermore, we evaluated the effect of patient and healthy urine on human podocyte cells. We conclude that cytokines abundant in the urine of AKI patients trigger processes that are needed to repair the damaged nephron and activate TP53 and SIRT1 to maintain the balance between proliferation, angiogenesis, and cell cycle arrest.
Potential Role of EPSPS Mutations in the Resistance of Eleusine indica to Glyphosate
Authors: Chen Jingchao , Cui Hailan , Li Zhiling , Yu Haiyan , Hou Qiang , Li Xiangju
Published: May 2023

Gene mutation is a basic evolutionary mechanism in plants under selection pressure of herbicides. Such mutation has pleiotropic effects on plant growth. We systemically investigated the effects of Pro106Leu (P106L), Pro106Ser (P106S), and Thr102Ile + Pro106Ser (TIPS) mutations on EPSPS functionality and fitness traits in Eleusine indica at the biochemical and physiological levels. The affinity of natural EPSPS for glyphosate was 53.8 times higher than that for phosphoenolpyruvate (PEP), as revealed by the dissociation constant; the constant decreased in both the P106L (39.9-fold) and P106S (46.9-fold) mutants but increased in the TIPS (87.5-fold) mutant. The Km (PEP) values of the P106L, P106S, and TIPS mutants were 2.4-, 0.7-, and 4.1-fold higher than that of natural EPSPS, corresponding to resistance levels of 2.5, 1.9, and 11.4, respectively. The catalytic efficiency values (maximum reaction rates) were 0.89-, 0.94-, and 0.26-fold higher than that of natural EPSPS. The levels of metabolites related to amino acids and nucleotides were significantly reduced in the mutated plants. The fitness costs were substantial for the biomass, total leaf area, seed number, and seedling emergence throughout the growth period in the plants with P106L and TIPS mutations. These results provide insights into EPSPS kinetics and their effect on plant growth.
Heck Macrocyclization in Forging Non-Natural Large Rings including Macrocyclic Drugs
Authors: Cai Jiayou , Sun Bin , Yu Siqi , Zhang Han , Zhang Weicheng
Published: May 2023

The intramolecular Heck reaction is a well-established strategy for natural product total synthesis. When constructing large rings, this reaction is also referred to as Heck macrocyclization, which has proved a viable avenue to access diverse naturally occurring macrocycles. Less noticed but likewise valuable, it has created novel macrocycles of non-natural origin that neither serve as nor derive from natural products. This review presents a systematic account of the title reaction in forging this non-natural subset of large rings, thereby addressing a topic rarely covered in the literature. Walking through two complementary sections, namely (1) drug discovery research and (2) synthetic methodology development, it demonstrates that beyond the well-known domain of natural product synthesis, Heck macrocyclization also plays a remarkable role in forming synthetic macrocycles, in particular macrocyclic drugs.
Increased Chymase-Positive Mast Cells in High-Grade Mucoepidermoid Carcinoma of the Parotid Gland
Authors: Nishimura Hiromi , Jin Denan , Kinoshita Ichita , Taniuchi Masataka , Higashino Masaaki , Terada Tetsuya , Takai Shinji , Kawata Ryo
Published: May 2023

It has long been known that high-grade mucoepidermoid carcinoma (MEC) has a poor prognosis, but the detailed molecular and biological mechanisms underlying this are not fully understood. In the present study, the pattern of chymase-positive mast cells, as well as chymase gene expression, in high-grade MEC was compared to that of low-grade and intermediate-grade MEC by using 44 resected tumor samples of MEC of the parotid gland. Chymase expression, as well as chymase-positive mast cells, was found to be markedly increased in high-grade MEC. Significant increases in PCNA-positive cells and VEGF gene expression, as well as lymphangiogenesis, were also confirmed in high-grade MEC. Chymase substrates, such as the latent transforming growth factor-beta (TGF-β) 1 and pro-matrix metalloproteinase (MMP)-9, were also detected immunohistologically in high-grade MEC. These findings suggested that the increased chymase activity may increase proliferative activity, as well as metastasis in the malignant condition, and the inhibition of chymase may be a strategy to improve the poor prognosis of high-grade MEC of the parotid gland.
TTT (Tel2-Tti1-Tti2) Complex, the Co-Chaperone of PIKKs and a Potential Target for Cancer Chemotherapy
Authors: Bhadra Sankhadip , Xu Yong-jie
Published: May 2023

The heterotrimeric Tel2-Tti1-Tti2 or TTT complex is essential for cell viability and highly conserved in eukaryotes. As the co-chaperone of ATR, ATM, DNA-PKcs, mTOR, SMG1, and TRRAP, the phosphatidylinositol 3-kinase-related kinases (PIKKs) and a group of large proteins of 300–500 kDa, the TTT plays crucial roles in genome stability, cell proliferation, telomere maintenance, and aging. Most of the protein kinases in the kinome are targeted by co-chaperone Cdc37 for proper folding and stability. Like Cdc37, accumulating evidence has established the mechanism by which the TTT interacts with chaperone Hsp90 via R2TP (Rvb1-Rvb2-Tah1-Pih1) complex or other proteins for co-translational maturation of the PIKKs. Recent structural studies have revealed the α-solenoid structure of the TTT and its interactions with the R2TP complex, which shed new light on the co-chaperone mechanism and provide new research opportunities. A series of mutations of the TTT have been identified that cause disease syndrome with neurodevelopmental defects, and misregulation of the TTT has been shown to contribute to myeloma, colorectal, and non-small-cell lung cancers. Surprisingly, Tel2 in the TTT complex has recently been found to be a target of ivermectin, an antiparasitic drug that has been used by millions of patients. This discovery provides mechanistic insight into the anti-cancer effect of ivermectin and thus promotes the repurposing of this Nobel-prize-winning medicine for cancer chemotherapy. Here, we briefly review the discovery of the TTT complex, discuss the recent studies, and describe the perspectives for future investigation.
Recent Studies on the Application of Microwave-Assisted Method for the Preparation of Heterogeneous Catalysts and Catalytic Hydrogenation Processes
Authors: Strekalova Anna A. , Shesterkina Anastasiya A. , Kustov Alexander L. , Kustov Leonid M.
Published: May 2023

Currently, microwave radiation is widely used in various chemical processes in order to intensify them and carry out processes within the framework of “green” chemistry approaches. In the last 10 years, there has been a significant increase in the number of scientific publications on the application of microwaves in catalytic reactions and synthesis of nanomaterials. It is known that heterogeneous catalysts obtained under microwave activation conditions have many advantages, such as improved catalytic characteristics and stability, and the synthesis of nanomaterials is accelerated several times compared to traditional methods used to produce catalysts. The present review article is to summarize the results of modern research on the use of microwave radiation for the synthesis of heterogeneous catalytic nanomaterials and discusses the prospects for research in the field of microwave-induced liquid-phase heterogeneous catalysis in hydrogenation.
3D ECM-Based Scaffolds Boost Young Cell Secretome-Derived EV Rejuvenating Effects in Senescent Cells
Authors: Arcuri Sharon , Pennarossa Georgia , De Iorio Teresina , Gandolfi Fulvio , Brevini Tiziana A. L.
Published: May 2023

Aging is a complex, multifaceted degenerative process characterized by a progressive accumulation of macroscopic and microscopic modifications that cause a gradual decline of physiological functions. During the last few years, strategies to ease and counteract senescence or even rejuvenate cells and tissues were proposed. Here we investigate whether young cell secretome-derived extracellular vesicles (EVs) ameliorate the cellular and physiological hallmarks of aging in senescent cells. In addition, based on the assumption that extracellular matrix (ECM) provides biomechanical stimuli, directly influencing cell behavior, we examine whether ECM-based bio-scaffolds, obtained from decellularized ovaries of young swine, stably maintain the rejuvenated phenotype acquired by cells after exposure to young cell secretome. The results obtained demonstrate that young cells release EVs endowed with the ability to counteract aging. In addition, comparison between young and aged cell secretomes shows a significantly higher miR-200 content in EVs produced using fibroblasts isolated from young donors. The effect exerted by young cell secretome-derived EVs is transient, but can be stabilized using a young ECM microenvironment. This finding indicates a synergistic interaction occurring among molecular effectors and ECM-derived stimuli that cooperate to control a unique program, driving the cell clock. The model described in this paper may represent a useful tool to finely dissect the complex regulations and multiple biochemical and biomechanical cues driving cellular biological age.
Structure and Assembly of the Proteus mirabilis Flagellar Motor by Cryo-Electron Tomography
Authors: Kaplan Mohammed , Yao Qing , Jensen Grant J.
Published: May 2023

Proteus mirabilis is a Gram-negative Gammaproteobacterium and a major causative agent of urinary tract infections in humans. It is characterized by its ability to switch between swimming motility in liquid media and swarming on solid surfaces. Here, we used cryo-electron tomography and subtomogram averaging to reveal the structure of the flagellar motor of P. mirabilis at nanometer resolution in intact cells. We found that P. mirabilis has a motor that is structurally similar to those of Escherichia coli and Salmonella enterica, lacking the periplasmic elaborations that characterize other more specialized gammaproteobacterial motors. In addition, no density corresponding to stators was present in the subtomogram average suggesting that the stators are dynamic. Finally, several assembly intermediates of the motor were seen that support the inside-out assembly pathway.
Inorganic Compounds as Remineralizing Fillers in Dental Restorative Materials: Narrative Review
Authors: Bin-Jardan Leena Ibraheem , Almadani Dalal Ibrahim , Almutairi Leen Saleh , Almoabid Hadi A. , Alessa Mohammed A. , Almulhim Khalid S. , AlSheikh Rasha N. , Al-Dulaijan Yousif A. , Ibrahim Maria S. , Al-Zain Afnan O. , Balhaddad Abdulrahman A.
Published: May 2023

Secondary caries is one of the leading causes of resin-based dental restoration failure. It is initiated at the interface of an existing restoration and the restored tooth surface. It is mainly caused by an imbalance between two processes of mineral loss (demineralization) and mineral gain (remineralization). A plethora of evidence has explored incorporating several bioactive compounds into resin-based materials to prevent bacterial biofilm attachment and the onset of the disease. In this review, the most recent advances in the design of remineralizing compounds and their functionalization to different resin-based materials’ formulations were overviewed. Inorganic compounds, such as nano-sized amorphous calcium phosphate (NACP), calcium fluoride (CaF2), bioactive glass (BAG), hydroxyapatite (HA), fluorapatite (FA), and boron nitride (BN), displayed promising results concerning remineralization, and direct and indirect impact on biofilm growth. The effects of these compounds varied based on these compounds’ structure, the incorporated amount or percentage, and the intended clinical application. The remineralizing effects were presented as direct effects, such as an increase in the mineral content of the dental tissue, or indirect effects, such as an increase in the pH around the material. In some of the reported investigations, inorganic remineralizing compounds were combined with other bioactive agents, such as quaternary ammonium compounds (QACs), to maximize the remineralization outcomes and the antibacterial action against the cariogenic biofilms. The reviewed literature was mainly based on laboratory studies, highlighting the need to shift more toward testing the performance of these remineralizing compounds in clinical settings.
The Conditions Matter: The Toxicity of Titanium Trisulfide Nanoribbons to Bacteria E. coli Changes Dramatically Depending on the Chemical Environment and the Storage Time
Authors: Zakharova Olga V. , Belova Valeria V. , Baranchikov Peter A. , Kostyakova Anna A. , Muratov Dmitry S. , Grigoriev Gregory V. , Chebotaryova Svetlana P. , Kuznetsov Denis V. , Gusev Alexander A.
Published: May 2023

In this work, we present an analysis of the antibacterial activity of TiS3 nanostructures in water and 0.9% NaCl solution suspensions. TiS3 nanoribbons 1–10 µm long, 100–300 nm wide, and less than 100 nm thick were produced by the direct reaction of pure titanium powder with elemental sulphur in a quartz tube sealed under vacuum. For the toxicity test of a bioluminescent strain of E. coli we used concentrations from 1 to 0.0001 g L−1 and also studied fresh suspensions and suspensions left for 24 h. The strongest toxic effect was observed in freshly prepared water solutions where the luminescence of bacteria decreased by more than 75%. When saline solution was substituted for water or when the solutions were stored for 24 h it resulted in a considerable decrease in the TiS3 antibacterial effect. The toxicity of TiS3 in water exceeded the toxicity of the reference TiO2 nanoparticles, though when saline solution was used instead of water the opposite results were observed. In addition, we did not find a relationship between the antibacterial activity of water suspensions of nanoribbons and the stability of their colloidal systems, which indicates an insignificant contribution to the toxicity of aggregation processes. In 0.9% NaCl solution suspensions, toxicity increased in proportion to the increase in the zeta potential. We suppose that the noted specificity of toxicity is associated with the emission of hydrogen sulphide molecules from the surface of nanoribbons, which, depending on the concentration, can either decrease or increase oxidative stress, which is considered the key mechanism of nanomaterial cytotoxicity. However, the exact underlying mechanisms need further investigation. Thus, we have shown an important role of the dispersion medium and the period of storage in the antibacterial activity of TiS3 nanoribbons. Our results could be used in nanotoxicological studies of other two-dimensional nanomaterials, and for the development of novel antibacterial substances and other biomedical applications of this two-dimensional material.
Common Clinical and Molecular Pathways between Migraine and Sarcoidosis
Authors: Tana Claudio , Azorin David Garcia , Cinetto Francesco , Mantini Cesare , Tana Marco , Caulo Massimo , Ricci Fabrizio , Martelletti Paolo , Cipollone Francesco , Giamberardino Maria Adele
Published: May 2023

Migraine and sarcoidosis are two distinct medical conditions that may have some common biological and clinical pathways. Sarcoidosis is a chronic granulomatous disease characterized by the formation of granulomas in various organs, including the lungs, skin, cardiovascular system, lymph nodes, and brain. Migraine is a common comorbidity in sarcoidosis patients and a common neurological disorder characterized by recurrent headaches that can be accompanied by other symptoms, such as nausea, vomiting, and sensitivity to light and sound. There have been several reports of individuals with neurosarcoidosis experiencing migraines, though the exact relationship between the two disorders is not well understood. Both conditions have been associated with inflammation and the activation of the immune system. In sarcoidosis, the formation of granulomas is thought to be an immune response to the presence of an unknown antigen. Similarly, the pain and other symptoms associated with migraines are thought to be caused by inflammation in the brain and the surrounding blood vessels. There is also evidence to suggest an interplay of environmental and genetic factors playing a role in both conditions, but evidence is inconsistent with the hypothesis of shared genetic susceptibility. This review aims to illustrate common clinical and biological pathways between migraine and sarcoidosis, including inflammation and dysregulation of the immune system, with a focus on the cumulative burden of concurrent disorders and therapeutic implications.
Risk Effects of rs1799945 Polymorphism of the HFE Gene and Intergenic Interactions of GWAS-Significant Loci for Arterial Hypertension in the Caucasian Population of Central Russia
Authors: Ivanova Tatiana , Churnosova Maria , Abramova Maria , Ponomarenko Irina , Reshetnikov Evgeny , Aristova Inna , Sorokina Inna , Churnosov Mikhail
Published: May 2023

The aim of this case-control replicative study was to investigate the link between GWAS-impact for arterial hypertension (AH) and/or blood pressure (BP) gene polymorphisms and AH risk in Russian subjects (Caucasian population of Central Russia). AH (n = 939) and control (n = 466) cohorts were examined for ten GWAS AH/BP risk loci. The genotypes/alleles of these SNP and their combinations (SNP–SNP interactions) were tested for their association with the AH development using a logistic regression statistical procedure. The genotype GG of the SNP rs1799945 (C/G) HFE was strongly linked with an increased AH risk (ORrecGG = 2.53; 95%CIrecGG1.03–6.23; ppermGG = 0.045). The seven SNPs such as rs1173771 (G/A) AC026703.1, rs1799945 (C/G) HFE, rs805303 (G/A) BAG6, rs932764 (A/G) PLCE1, rs4387287 (C/A) OBFC1, rs7302981 (G/A) CERS5, rs167479 (T/G) RGL3, out of ten regarded loci, were related with AH within eight SNP–SNP interaction models (<0.001 ≤ pperm-interaction ≤ 0.047). Three polymorphisms such as rs8068318 (T/C) TBX2, rs633185 (C/G) ARHGAP42, and rs2681472 (A/G) ATP2B1 were not linked with AH. The pairwise rs805303 (G/A) BAG6–rs7302981 (G/A) CERS5 combination was a priority in determining the susceptibility to AH (included in six out of eight SNP–SNP interaction models [75%] and described 0.82% AH entropy). AH-associated variants are conjecturally functional for 101 genes involved in processes related to the immune system (major histocompatibility complex protein, processing/presentation of antigens, immune system process regulation, etc.). In conclusion, the rs1799945 polymorphism of the HFE gene and intergenic interactions of BAG6, CERS5, AC026703.1, HFE, PLCE1, OBFC1, RGL3 have been linked with AH risky in the Caucasian population of Central Russia.
Rivastigmine–Benzimidazole Hybrids as Promising Multitarget Metal-Modulating Compounds for Potential Treatment of Neurodegenerative Diseases
Authors: Vicente-Zurdo David , Brunetti Leonardo , Piemontese Luca , Guedes Beatriz , Cardoso Sandra M. , Chavarria Daniel , Borges Fernanda , Madrid Yolanda , Chaves Sílvia , Santos M. Amélia
Published: May 2023

With the goal of combating the multi-faceted Alzheimer’s disease (AD), a series of Rivastigmine-Benzimidazole (RIV–BIM) hybrids was recently reported by us as multitarget-directed ligands, thanks to their capacity to tackle important hallmarks of AD. In particular, they exhibited antioxidant activity, acted as cholinesterase inhibitors, and inhibited amyloid-β (Aβ) aggregation. Herein, we moved forward in this project, studying their ability to chelate redox-active biometal ions, Cu(II) and Fe(III), with widely recognized roles in the generation of oxidative reactive species and in protein misfolding and aggregation in both AD and Parkinson’s disease (PD). Although Cu(II) chelation showed higher efficiency for the positional isomers of series 5 than those of series 4 of the hybrids, the Aβ-aggregation inhibition appears more dependent on their capacity for fibril intercalation than on copper chelation. Since monoamine oxidases (MAOs) are also important targets for the treatment of AD and PD, the capacity of these hybrids to inhibit MAO-A and MAO-B was evaluated, and they showed higher activity and selectivity for MAO-A. The rationalization of the experimental evaluations (metal chelation and MAO inhibition) was supported by computational molecular modeling studies. Finally, some compounds showed also neuroprotective effects in human neuroblastoma (SH-SY5Y cells) upon treatment with 1-methyl-4-phenylpyridinium (MPP+), a neurotoxic metabolite of a Parkinsonian-inducing agent.
Two-Dimensional Numerical Analysis for TBM Tunneling-Induced Structure Settlement: A Proposed Modeling Method and Parametric Study
Authors: Alsirawan Rashad , Sheble Ashraf , Alnmr Ammar
Published: May 2023

The construction of tunnels in densely populated urban areas poses a significant challenge in terms of anticipating the settlement that may result from tunnel excavation. This paper presents a new and more realistic modeling method for tunnel excavation using a Tunnel Boring Machine (TBM). This method is compared with other reference modeling methods using a validated model of a subsurface tunnel excavated by a TBM with a slurry shield. A parametric study is conducted to investigate the impact of key parameters, including structure width, foundation depth, eccentricity, load on the structure, overburden depth, and tunnel diameter, on tunnel–soil–structure interaction and the resulting structure settlements. The results reveal that the tunnel diameter, eccentricity, and overburden depth have a significant impact on structure settlements, with values of 22.5%, 17%, and 7.1%, respectively. Finally, the paper proposes an equation for predicting the maximum settlement of a structure, considering the critical parameters. The validity of the equation is evaluated by comparing its results with the outputs from various case studies, including a newly validated model, two real-life case studies, and centrifuge tests. The results indicate a high level of consistency between the calculated and measured settlements.
Bulk Tungsten Fiber-Reinforced Tungsten (Wf/W) Composites Using Yarn-Based Textile Preforms
Authors: Lau Alexander , Coenen Jan Willem , Schwalenberg Daniel , Mao Yiran , Höschen Till , Riesch Johann , Raumann Leonard , Treitz Michael , Gietl Hanns , Terra Alexis , Göhts Beatrix , Linsmeier Christian , Theis-Bröhl Katharina , Gonzalez-Julian Jesus
Published: May 2023

The use of tungsten fiber-reinforced tungsten composites (Wf/W) has been demonstrated to significantly enhance the mechanical properties of tungsten (W) by incorporating W-fibers into the W-matrix. However, prior research has been restricted by the usage of single fiber-based textile fabrics, consisting of 150 µm warp and 50 µm weft filaments, with limited homogeneity, reproducibility, and mechanical properties in bulk structures due to the rigidity of the 150 µm W-fibers. To overcome this limitation, two novel textile preforms were developed utilizing radial braided W-yarns with 7 core and 16 sleeve filaments (R.B. 16 + 7), with a diameter of 25 µm each, as the warp material. In this study, bulk composites of two different fabric types were produced via a layer-by-layer CVD process, utilizing single 50 µm filaments (type 1) and R.B. 16 + 7 yarns (type 2) as weft materials. The produced composites were sectioned into KLST-type specimens based on DIN EN ISO 179-1:2000 using electrical discharge machining (EDM) and subjected to three-point bending tests. Both composites demonstrated enhanced mechanical properties with pseudo-ductile behavior at room temperature and withstood over 10,000 load cycles between 50–90% of their respective maximum load without sample fracture in three-point cyclic loading tests. Furthermore, a novel approach to predict the fatigue behavior of the material under cyclic loading was developed based on the high reproducibility of the composites produced, especially for the composite based on type 1. This approach provides a new benchmark for upscaling endeavors and may enable a better prediction of the service life of the produced components made of Wf/W in the future. In comparison, the composite based on fabric type 1 demonstrated superior results in manufacturing performance and mechanical properties. With a high relative average density (>97%), a high fiber volume fraction (14–17%), and a very homogeneous fiber distribution in the CVD-W matrix, type 1 shows a promising option to be further tested in high heat flux tests and to be potentially used as an alternative to currently used materials for the most stressed components of nuclear fusion reactors or other potential application fields such as concentrated solar power (CSP), aircraft turbines, the steel industry, quantum computing, or welding tools. Type 2 composites have a higher layer spacing compared to type 1, resulting in gaps within the matrix and less homogeneous material properties. While type 2 composites have demonstrated a notable enhancement over 150 µm fiber-based composites, they are not viable for industrial scale-up unlike type 1 composites.
Solvent- and Co-Catalyst-Free Cycloaddition of Carbon Dioxide and Epoxides Catalyzed by Recyclable Bifunctional Niobium Complexes
Authors: Wen Qin , Yuan Xuexin , Zhou Qiqi , Yang Hai-Jian , Jiang Qingqing , Hu Juncheng , Guo Cun-Yue
Published: May 2023

CO2, as a cheap and abundant renewable C1 resource, can be used to synthesize high value-added chemicals. In this paper, a series of bifunctional metallic niobium complexes were synthesized and their structures were characterized by IR, NMR and elemental analysis. All of these complexes have been proved to be efficient catalysts for the coupling reaction of CO2 and epoxides to obtain cyclic carbonates under solvent- and co-catalyst-free conditions. By using CO2 and propylene oxide as a model reaction, the optimal reaction conditions were systematically screened as: 100 °C, 1 MPa, 2 h, ratio of catalyst to alkylene oxide 1:100. Under the optimal reaction conditions, the bifunctional niobium catalysts can efficiently catalyze the coupling reaction with high yield and excellent selectivity (maximum yield of >99% at high pressure and 96.8% at atmospheric pressure). Moreover, this series of catalysts can also catalyze the coupling reaction at atmospheric pressure and most of them showed high conversion of epoxide. The catalysts have good substrate suitability and are also applicable to a variety of epoxides including diepoxides and good catalytic performances were achieved for producing the corresponding cyclic carbonates in most cases. Furthermore, the catalysts can be easily recovered by simple filtration and reused for at least five times without obvious loss of catalytic activity and selectivity. Kinetic studies were carried out preliminarily for the bifunctional niobium complexes with different halogen ions (3a(Cl−), 3b(Br−), 3c(I−)) and the formation activation energies (Ea) of cyclic carbonates were obtained. The order of apparent activation energy Ea is 3a (96.2 kJ/mol) > 3b (68.2 kJ/mol) > 3c (37.4 kJ/mol). Finally, a possible reaction mechanism is proposed.
Wood-Decaying Fungi: From Timber Degradation to Sustainable Insulating Biomaterials Production
Authors: Charpentier-Alfaro Camila , Benavides-Hernández Jorge , Poggerini Marco , Crisci Alfonso , Mele Giacomo , Della Rocca Gianni , Emiliani Giovanni , Frascella Angela , Torrigiani Tommaso , Palanti Sabrina
Published: May 2023

Addressing the impacts of climate change and global warming has become an urgent priority for the planet’s well-being. In recent decades the great potential of fungal-based products with characteristics equal to, or even outperforming, classic petroleum-derived products has been acknowledged. These new materials present the added advantage of having a reduced carbon footprint, less environmental impact and contributing to the shift away from a fossil-based economy. This study focused on the production of insulation panels using fungal mycelium and lignocellulosic materials as substrates. The process was optimized, starting with the selection of Trametes versicolor, Pleurotus ostreatus, P. eryngii, Ganoderma carnosum and Fomitopsis pinicola isolates, followed by the evaluation of three grain spawn substrates (millet, wheat and a 1:1 mix of millet and wheat grains) for mycelium propagation, and finishing with the production of various mycelium-based composites using five wood by-products and waste materials (pine sawdust, oak shavings, tree of heaven wood chips, wheat straw and shredded beech wood). The obtained biomaterials were characterized for internal structure by X-ray micro-CT, thermal transmittance using a thermoflowmeter and moisture absorption. The results showed that using a wheat and millet 1:1 (w/w) mix is the best option for spawn production regardless of the fungal isolate. In addition, the performance of the final composites was influenced both by the fungal isolate and the substrate used, with the latter having a stronger effect on the measured properties. The study shows that the most promising sustainable insulating biomaterial was created using T. versicolor grown on wheat straw.
Direct Energy Deposition Parametric Simulation Investigation in Gear Repair Applications
Authors: Ferreira Nuno Miguel , Vila Pouca Maria , Fernandes Carlos , Seabra Jorge , Lesiuk Grzegorz , Parente Marco , Jesus Abílio
Published: May 2023

Additive manufacturing technologies have numerous advantages over conventional technologies; nevertheless, their production process can lead to high residual stresses and distortions in the produced parts. The use of numerical simulation models is presented as a solution to predict the deformations and residual stresses resulting from the printing process. This study aimed to predict the tensions and distortions imposed in the gear repair process by directed energy deposition (DED). First, the case study proposed by National Institute of Standards and Technology (NIST) was analyzed to validate the model and the numerically obtained results. Subsequently, a parametric study of the influence of some of the parameters of DED technology was carried out. The results obtained for the validation of the NIST benchmark bridge model were in agreement with the results obtained experimentally. In turn, the results obtained from the parametric study were almost always in line with what is theoretically expected; however, some results were not very clear and consistent. The results obtained help to clarify the influence of certain printing parameters. The proposed model allowed accounting for the effect of residual stresses in calculating the stresses resulting from gear loading, which are essential data for fatigue analysis. Modeling and simulating a deposition process can be challenging due to several factors, including calibrating the model, managing the computational cost, accounting for boundary conditions, and accurately representing material properties. This paper aimed to carefully address these parameters in two case studies, towards reliable simulations.
Loss Mitigation in Self-Biased Microstrip Circulators
Authors: Kong Lingqi , Schuchinsky Alexander , Joseph Sumin , Eker Taylan , Huang Yi
Published: May 2023

Integration of the ferrite devices in the RF front-end and active antennas is hindered by the need for external magnets, biasing soft microwave ferrites. The hexaferrite-based self-biased nonreciprocal devices can operate without external magnets at mm-wave frequencies but the currently available hexaferrite materials inflict high RF losses at lower frequencies, particularly in the wireless communication bands. In this paper, the parameters of La-Co-substituted hexaferrite compounds are used for the self-biased circulators in the low GHz frequency bands, and a means of the dissipation loss reduction are discussed.
Structural Manipulations of Marine Natural Products Inspire a New Library of 3-Amino-1,2,4-Triazine PDK Inhibitors Endowed with Antitumor Activity in Pancreatic Ductal Adenocarcinoma
Authors: Carbone Daniela , De Franco Michele , Pecoraro Camilla , Bassani Davide , Pavan Matteo , Cascioferro Stella , Parrino Barbara , Cirrincione Girolamo , Dall’Acqua Stefano , Sut Stefania , Moro Stefano , Gandin Valentina , Diana Patrizia
Published: May 2023

Pancreatic ductal adenocarcinoma (PDAC) is one of the main aggressive types of cancer, characterized by late prognosis and drug resistance. Among the main factors sustaining PDAC progression, the alteration of cell metabolism has emerged to have a key role in PDAC cell proliferation, invasion, and resistance to standard chemotherapeutic agents. Taking into account all these factors and the urgency in evaluating novel options to treat PDAC, in the present work we reported the synthesis of a new series of indolyl-7-azaindolyl triazine compounds inspired by marine bis-indolyl alkaloids. We first assessed the ability of the new triazine compounds to inhibit the enzymatic activity of pyruvate dehydrogenase kinases (PDKs). The results showed that most of derivatives totally inhibit PDK1 and PDK4. Molecular docking analysis was executed to predict the possible binding mode of these derivatives using ligand-based homology modeling technique. Evaluation of the capability of new triazines to inhibit the cell growth in 2D and 3D KRAS-wild-type (BxPC-3) and KRAS-mutant (PSN-1) PDAC cell line, was carried out. The results showed the capacity of the new derivatives to reduce cell growth with a major selectivity against KRAS-mutant PDAC PSN-1 on both cell models. These data demonstrated that the new triazine derivatives target PDK1 enzymatic activity and exhibit cytotoxic effects on 2D and 3D PDAC cell models, thus encouraging further structure manipulation for analogs development against PDAC.
Female Gonadal Venous Insufficiency in a Clinical Presentation Which Suggested an Acute Abdomen—A Case Report and Literature Review
Authors: Matei Sergiu-Ciprian , Dumitru Cristina Ștefania , Oprițoiu Andrei-Ion , Marian Lucian , Murariu Marius-Sorin , Olariu Sorin
Published: May 2023

Pelvic venous insufficiency (PVI) is frequently associated with symptoms of abdominal pain or discomfort that is overlooked or under-diagnosed in women. Despite the fact that pelvic venous insufficiency in men is very well documented, its occurrence in women needs to be further studied. Patients with pelvic varicose veins undergo a long and inconclusive diagnostic work-up before the exact cause of the symptoms is identified. Gonadal venous insufficiency (GVI) is a condition that can present acutely, leading to diagnostic challenges. We present a case report of a 47-year-old female with acute abdominal pain and GVI, where endovascular embolization was used for successful treatment. The patient was diagnosed with GVI based on imaging findings of an enlarged left ovarian vein with retrograde flow and dilated pelvic veins seen on magnetic resonance imaging (MRI) with contrast material. Due to the severity of her symptoms and imaging findings, endovascular embolization was chosen as the treatment modality. The embolization was successful, and the patient’s symptoms resolved completely. This case highlights the challenge of diagnosing GVI with acute clinical expression and the potential benefits of endovascular embolization as a treatment option. Further studies are needed to determine the optimal management strategies for acute GVI, but endovascular embolization should be considered a safe and effective option. At the same time, we present a short review of the recent literature data related to this topic.
Novel Material Obtained from the Gasification Residues of Candiota Mine’s Coal for Cationic Dye Adsorption
Authors: Lucena Gabriella , Rodrigues Tereza Longaray , da Rosa Josué Vieira , da Rosa Gabriela Silveira , Muniz Ana Rosa Costa
Published: May 2023

This work aims to prepare and characterize the unburned carbon obtained from gasification residues and evaluate its application as an adsorbent for the removal of textile dye contaminants. The results of physical and chemical properties showed a specific mass of 2.05 g/cm3, surface area of 23.983 g/cm2, and diameter and pore volume of 0.844 nm and 2.262 cm3/g, respectively. These properties, along with the point of zero charge and chemical bonds present on the surface, favored the adsorption of cationic dyes. The adsorption results showed great potential for the removal of methylene blue, crystal violet, and basic fuchsin if compared with bromocresol green, and indigo carmine. The maximum removal values obtained for methylene blue were up to 99% and the kinetic adsorption was faster at the beginning of the process, reaching the equilibrium in less than 5 min. The results obtained through the adsorption isotherms showed a maximum adsorption capacity of 333.33 and 476.19 mg/g, at the temperature of 291 and 328 K, respectively. The satisfactory results showed that the use of unburned carbon is a cost-effective and eco-friendly alternative to reusing the residue from gasification and also contributes to the decontamination of watercourses.
E2E-RDS: Efficient End-to-End Ransomware Detection System Based on Static-Based ML and Vision-Based DL Approaches
Authors: Almomani Iman , Alkhayer Aala , El-Shafai Walid
Published: May 2023

Nowadays, ransomware is considered one of the most critical cyber-malware categories. In recent years various malware detection and classification approaches have been proposed to analyze and explore malicious software precisely. Malware originators implement innovative techniques to bypass existing security solutions. This paper introduces an efficient End-to-End Ransomware Detection System (E2E-RDS) that comprehensively utilizes existing Ransomware Detection (RD) approaches. E2E-RDS considers reverse engineering the ransomware code to parse its features and extract the important ones for prediction purposes, as in the case of static-based RD. Moreover, E2E-RDS can keep the ransomware in its executable format, convert it to an image, and then analyze it, as in the case of vision-based RD. In the static-based RD approach, the extracted features are forwarded to eight various ML models to test their detection efficiency. In the vision-based RD approach, the binary executable files of the benign and ransomware apps are converted into a 2D visual (color and gray) images. Then, these images are forwarded to 19 different Convolutional Neural Network (CNN) models while exploiting the substantial advantages of Fine-Tuning (FT) and Transfer Learning (TL) processes to differentiate ransomware apps from benign apps. The main benefit of the vision-based approach is that it can efficiently detect and identify ransomware with high accuracy without using data augmentation or complicated feature extraction processes. Extensive simulations and performance analyses using various evaluation metrics for the proposed E2E-RDS were investigated using a newly collected balanced dataset that composes 500 benign and 500 ransomware apps. The obtained outcomes demonstrate that the static-based RD approach using the AB (Ada Boost) model achieved high classification accuracy compared to other examined ML models, which reached 97%. While the vision-based RD approach achieved high classification accuracy, reaching 99.5% for the FT ResNet50 CNN model. It is declared that the vision-based RD approach is more cost-effective, powerful, and efficient in detecting ransomware than the static-based RD approach by avoiding feature engineering processes. Overall, E2E-RDS is a versatile solution for end-to-end ransomware detection that has proven its high efficiency from computational and accuracy perspectives, making it a promising solution for real-time ransomware detection in various systems.
The Application of a Self-Made Integrated Three-in-One Microsensor and Commercially Available Wind Speed Sensor to the Cold Air Pipe of the Heating, Ventilation, and Air Conditioning in a Factory for Real-Time Wireless Measurement
Authors: Lee Chi-Yuan , Shieh Jiann-Shing , Chen Jerry , Wang Xin-Wen , Liu Chen-Kai , Wei Chia-Hsin
Published: May 2023

In this study, the integrated three-in-one (temperature, humidity, and wind speed) microsensor was made through the technology of the Micro-electro-mechanical Systems (MEMS) to measure three important physical quantities of the internal environment of the cold air pipe of the Heating, Ventilation and Air Conditioning (HVAC) in the factory, plan the installation positions of the integrated three-in-one microsensor and commercially available wind speed sensor required by the internal environment of the cold air pipe, and conduct the actual 310-h long term test and comparison. In the experiment, it was also observed that the self-made micro wind speed sensor had higher stability compared to the commercially available wind speed sensor (FS7.0.1L.195). The self-made micro wind speed sensor has a variation range of ±200 mm/s, while the commercially available wind speed sensor a variation range of ±1000 mm/s. The commercially available wind speed sensor (FS7.0.1L.195) can only measure the wind speed; however, the self-made integrated three-in-one microsensor can conduct real-time measurements of temperature and humidity according to the environment at that time, and use different calibration curves to know the wind speed. As a result, it is more accurate and less costly than commercially available wind speed sensors. The material cost of self-made integrated three-in-one microsensor includes chemicals, equipment usage fees, and wires. In the future, factories may install a large number of self-made integrated three-in-one microsensors in place of commercially available wind speed sensors. Through real-time wireless measurements, the self-made integrated three-in-one microsensors can achieve the control optimization of the HVAC cold air pipe’s internal environment to improve the quality of manufactured materials.
DR-ALOHA-Q: A Q-Learning-Based Adaptive MAC Protocol for Underwater Acoustic Sensor Networks
Authors: Tomovic Slavica , Radusinovic Igor
Published: May 2023

Underwater acoustic sensor networks (UASNs) are challenged by the dynamic nature of the underwater environment, large propagation delays, and global positioning system (GPS) signal unavailability, which make traditional medium access control (MAC) protocols less effective. These factors limit the channel utilization and performance of UASNs, making it difficult to achieve high data rates and handle congestion. To address these challenges, we propose a reinforcement learning (RL) MAC protocol that supports asynchronous network operation and leverages large propagation delays to improve the network throughput.he protocol is based on framed ALOHA and enables nodes to learn an optimal transmission strategy in a fully distributed manner without requiring detailed information about the external environment. The transmission strategy of sensor nodes is defined as a combination of time-slot and transmission-offset selection. By relying on the concept of learning through interaction with the environment, the proposed protocol enhances network resilience and adaptability. In both static and mobile network scenarios, it has been compared with the state-of-the-art framed ALOHA for the underwater environment (UW-ALOHA-Q), carrier-sensing ALOHA (CS-ALOHA), and delay-aware opportunistic transmission scheduling (DOTS) protocols. The simulation results show that the proposed solution leads to significant channel utilization gains, ranging from 13% to 106% in static network scenarios and from 23% to 126% in mobile network scenarios.oreover, using a more efficient learning strategy, it significantly reduces convergence time compared to UW-ALOHA-Q in larger networks, despite the increased action space.
Object Affordance-Based Implicit Interaction for Wheelchair-Mounted Robotic Arm Using a Laser Pointer
Authors: Liu Yaxin , Liu Yan , Yao Yufeng , Zhong Ming
Published: May 2023

With the growth of the world’s population, limited healthcare resources cannot provide adequate nursing services for all people in need. The wheelchair-mounted robotic arm (WMRA) with interactive technology could help to improve users’ self-care ability and relieve nursing stress. However, the users struggle to control the WMRA due to complex operations. To use the WMRA with less burden, this paper proposes an object affordance-based implicit interaction technology using a laser pointer. Firstly, a laser semantic identification algorithm combined with the YOLOv4 and the support vector machine (SVM) is designed to identify laser semantics. Then, an implicit action intention reasoning algorithm, based on the concept of object affordance, is explored to infer users’ intentions and learn their preferences. For the purpose of performing the actions about task intention in the scene, the dynamic movement primitives (DMP) and the finite state mechanism (FSM) are respectively used to generalize the trajectories of actions and reorder the sequence of actions in the template library. In the end, we verified the feasibility of the proposed technology on a WMRA platform. Compared with the previous method, the proposed technology can output the desired intention faster and significantly reduce the user’s limb involvement time (about 85%) in operating the WMRA under the same task.