bluuebunny/crossref_metadata_2025 huggingface dataset:
Correlation analysis of the abdominal visceral fat area with the structure and function of the heart and liver in obesity: a prospective magnetic resonance imaging study
Authors: Bai Jinquan , Gao Chao , Li Xiaolu , Pan Hong , Wang Shuting , Shi Zhenzhou , Zhang Tong
Published: August 2023

Background

The differences in fat deposition sites exhibit varying degrees of systemic inflammatory responses and organ damage, especially in obese individuals with excessive visceral fat. Visceral fat, which is closely related to an increase in mortality rates related to heart and liver diseases. However, few studies have analysed the differences in heart and liver indicators and their correlation among groups based on the abdominal visceral fat area (AVFA).

Objective

Clarifying the differences in and correlations of heart and liver indicators among groups with different severities of AVFA by magnetic resonance imaging (MRI).

Methods

Sixty-nine subjects with obesity were enrolled. The study group consisted of forty-one individuals (AVFA ≥ 150 cm2), and the control group consisted of twenty-eight individuals (100 cm2 ≤ AVFA < 150 cm2). The differences in and correlations between clinical, laboratory, and MRI indicators of the heart and liver between the two groups were analysed.

Results

In the study group, the incidences of type 2 diabetes mellitus (T2DM) and insulin resistance were higher, and liver function indicators were worse. The left ventricular eccentricity ratio (LVER), left ventricular mass (LVM) and global peak wall thickness (GPWT) were higher in the study group than in the control group (P = 0.002, P = 0.001, P = 0.03), and the left ventricle global longitudinal strain (LVGLS) was lower in the study group than in the control group (P = 0.016). The pericardiac adipose tissue volume (PATV) and myocardial proton density fat fraction (M-PDFF) were higher in the study group than in the control group (P = 0.001, P = 0.001). The hepatic proton density fat fraction (H-PDFF) and abdominal subcutaneous fat area (ASFA) were higher in the study group than in the control group (P < 0.001, P = 0.012). There was a moderate positive correlation (ρ = 0.39–0.59, P < 0.001) between the AVFA and LVER, LVM, GPWT, LVGLS, and H-PDFF. There was no difference in right ventricular and most left ventricular systolic and diastolic function between the two groups.

Conclusion

The high AVFA group had a larger LVM, GPWT and PATV, more obvious changes in LVER, impaired left ventricular diastolic function, an increased risk of heart disease, and more severe hepatic fat deposition and liver injury. Therefore, there is a correlation between the amount of visceral adipose tissue and subclinical cardiac changes and liver injury.
Validation of predictive equations for resting energy expenditure in children and adolescents with different body mass indexes
Authors: Acar-Tek Nilüfer , Ağagündüz Duygu , Şahin Teslime Özge , Baygut Hatice , Uzunlar Elif Adanur , Zakkour Hazal Küçükkaraca , Karaçallı Ayşegül
Published: August 2023

Background

Accurate estimation of resting energy expenditure (REE) in children and adolescents is important to establish estimated energy requirements. The objective of this study was to assess the validity of existing equations in literature and a newly developed equation in estimating REE in children and adolescents.

Methods

275 participants (148 boys, 127 girls) aged 6–18 years included in the study were classified as normal-weighted, overweight, obese based on BMI z-scores for age according to WHO-2007 growth curves for 5–19 years of age. REEs were measured using an indirect calorimeter, with various equations, and a newly established equation [REE = 505.412+(24.383*FFM);Adjusted R2 = 0.649] were compared with REE measured using Bland-Altman and further validation parameters.

Results

When the predicted REEs were compared with the measured REEs, the highest prediction accuracy was achieved using the new Eq. (64.8%) and IOM (63.8%) for normal-weight participants, Müller FFM and new Eq. (59.6%) for overweight participants and Lazzer (44.9%) for obese participants. In normal and overweight participants, lowest root mean squared error (RMSE) values were acquired from Schmelzle’s equation (respectively 136.2;159.9 kcal/d), and the highest values were found in Kim’s Eq. (315.2; 295.2 kcal/d respectively). RMSE value of the new equation was 174.7 kcal/d for normal-weight children and adolescents, and 201.9 kcal/d for overweight ones. In obese participants, the lowest RMSE value was obtained from Schmelzle’s Eq. (305.4 kcal/d) and the new Eq. (317.4 kcal/d), while the highest value was obtained from IOM Eq. (439.9 kcal/d). RMSE was higher in obese groups compared to the other BMI groups.

Conclusion

Indirect-calorimeter is the most suitable method for REE measurement in especially obese children and adolescents. The new equation and Schmelzle’s equation appear to be most accurate equations for normal and overweight children and adolescents.
Protein regulator of cytokinesis 1: a potential oncogenic driver
Authors: Li Sijing , Motiño Omar , Lambertucci Flavia , Martins Isabelle , Sun Li , Kroemer Guido
Published: August 2023

Protein regulator of cytokinesis 1 (PRC1) is involved in cytokinesis. Growing evidence suggests the association of PRC1 with multiple cancers. Here, we unveil that, in 28 cancer types, PRC1 is higher expressed in tumor tissues than in non-malignant tissues. Overexpression of PRC1 indicates unfavorable prognostic value, especially in ACC, LGG, KIRP, LICH, LUAD, MESO, PAAD, SARC and UCEC, while methylation of the PRC1 gene at sites associated with its inactivation has a favorable prognostic value in ACC, KIRP, LUAD, MESO, KIRP and LGG. Differentially expressed genes (DEGs) associated with high (> median) PRC1 expression contribute to key signaling pathways related with cell cycle, DNA damage and repair, EMT, cell migration, invasion and cell proliferation in most cancer types. More specifically, the DEGs involved in RAS/RAF/MAPK, PI3K/AKT, WNT, NOTCH, TGF-β, integrin, EMT process, focal adhesion, RHO GTPase-related pathway or microtubule cytoskeleton regulation are upregulated when PRC1 expression is above median, as confirmed for most cancers. Most importantly, high expression of PRC1 appears to be associated with an overabundance of poor-prognosis TH2 cells. Furthermore, positive correlations of PRC1 and some immune checkpoint genes (CD274, CTLA4, HAVCR2, LAG3, PDCD1, PDCD1LG2, TIGIT, and CD86) were observed in several cancers, especially BLCA, BRCA, KIRC, LUAD, LIHC, PRAD and THCA. These findings plead in favor of further studies validating the diagnostic and prognostic impact of PRC1 as well as the elaboration of pharmacological strategies for targeting PRC1.
Monoarticular juvenile idiopathic arthritis as a distinct clinical entity A proof-of-concept study
Authors: Zulian Francesco , Pierobon Beatrice , Zannin Maria Elisabetta , Politi Caterina , Vittadello Fabio , Meneghel Alessandra , Tirelli Francesca , Martini Giorgia
Published: August 2023

Background

Currently, monoarticular Juvenile Idiopathic Arthritis (monoJIA) is included in the ILAR classification as oligoarticular subtype although various aspects, from clinical practice, suggest it as a separate entity.

Objectives

To describe the clinical characteristics of persistent monoJIA.

Methods

Patients with oligoJIA and with at least two years follow-up entered the study. Those with monoarticular onset and persistent monoarticular course were compared with those with oligoJIA. Variables considered were: sex, age at onset, presence of benign joint hypermobility (BJH), ANA, uveitis, therapy and outcome. Patients who had not undergone clinical follow-up for more than 12 months were contacted by structured telephone interview.

Results

Of 347 patients with oligoJIA, 196 with monoarticular onset entered the study and 118 (60.2%), identified as persistent monoJIA, were compared with 229 oligoJIA. The mean follow-up was 11.4 years. The switch from monoarticular onset to oligoarticular course of 78 patients (38.8%) occurred by the first three years from onset. In comparison with oligoJIA, the most significant features of monoJIA were later age at onset (6.1 vs. 4.7 years), lower female prevalence (70.3 vs. 83.4%), higher frequency of BJH (61.9 vs. 46.3%), lower frequency of uveitis (14.4 vs. 34.1%) and ANA+ (68.6 vs. 89.5%) and better long-term outcome.

Conclusions

MonoJIA, defined as persistent arthritis of unknown origin of a single joint for at least three years, seems to be a separate clinical entity from oligoJIA. This evidence may be taken into consideration for its possible inclusion into the new classification criteria for JIA and open new therapeutic perspectives.
Evaluating rates of chiropractic use and utilization by patient sex within the United States Veterans Health Administration: a serial cross-sectional analysis
Authors: Graham Sarah E. , Coleman Brian C. , Zhao Xiwen , Lisi Anthony J.
Published: August 2023

Background

Within the United States Veterans Health Administration (VHA), the number of patients using healthcare services has increased over the past several decades. Females make up a small proportion of overall patients within the VHA; however, this proportion is growing rapidly. Previous studies have described rates of VHA chiropractic use; however, no prior study assessed differences in use or utilization rates between male and female veterans. The purpose of this study was to assess rates of use and utilization of chiropractic care by sex among VHA patients receiving care at VHA facilities with on-station chiropractic clinics.

Methods

A serial cross-sectional analysis of VHA national electronic health record data was conducted in Fall 2021 for fiscal year (FY) 2005–2021. The cohort population was defined as VHA facilities with on-station chiropractic clinics, and facilities were admitted to the cohort after the first FY with a minimum of 500 on-station chiropractic visits. Variables extracted included counts of unique users of any VHA on-station facility outpatient services, unique users of VHA on-station facility chiropractic services, number of chiropractic visits, and sex. To calculate use, we determined the proportion of patients of each sex who received chiropractic services to the total patients of the same sex receiving any outpatient care within each facility. To calculate utilization, we determined the number of chiropractic care visits per patient per fiscal year. A linear mixed effects model was applied to examine the difference in chiropractic care utilization by sex.

Results

The percentage of female VHA on-station chiropractic patients increased from 11.7 to 17.7% from FY2005–FY2021. Among VHA facilities with on-station chiropractic care, the percentage of female VHA healthcare users who used chiropractic care (mean = 2.3%) was greater than the percentage of male VHA healthcare users who used chiropractic care (mean = 1.1%). Rates of chiropractic utilization by sex among VHA facilities with on-station chiropractic clinics were slightly higher for females (median = 4.3 visits per year, mean = 4.9) compared to males (median = 4.1 visits per year, mean = 4.6).

Conclusion

We report higher use and utilization of VHA chiropractic care by females compared with males, yet for both sexes rates were lower than in the private US healthcare system. This highlights the need for further assessment of the determinants and outcomes of VHA chiropractic care.
Prioritizing children’s mental health amidst Sudan’s humanitarian crisis: policy recommendations for immediate action
Authors: Osman Sarah Hashim Mohammed , Nashwan Abdulqadir J.
Published: August 2023

The humanitarian crisis precipitated by the ongoing conflict in Sudan poses profound risks to the health and welfare of the country’s children. This paper explores essential policy interventions to safeguard child mental health services under these challenging circumstances. Crucial strategies include enhancing healthcare accessibility for children and their caregivers, promoting education, and improving household living conditions. Additionally, it is vital to provide improved access to information about nutritious food and strengthen health systems in areas directly exposed to conflict. Cooperation with international aid organizations is paramount to delivering medical supplies to functioning health facilities. The paper also recommends partnerships with local non-governmental and humanitarian organizations to execute public health programs effectively. These multi-faceted policy measures underscore the importance of a comprehensive response to ensure the health and well-being of children amid the turmoil in Sudan. Through these strategies, we aim to provide a blueprint for policymakers and humanitarian organizations to mitigate the devastating impacts of the conflict on the country’s most vulnerable population.
Mutant p53-ENTPD5 control of the calnexin/calreticulin cycle: a druggable target for inhibiting integrin-α5-driven metastasis
Authors: Pavlakis Evangelos , Neumann Michelle , Merle Nastasja , Wieboldt Ronja , Wanzel Michael , Ponath Viviane , Pogge von Strandmann Elke , Elmshäuser Sabrina , Stiewe Thorsten
Published: August 2023

Background

TP53, encoding the tumor suppressor p53, is frequently mutated in various cancers, producing mutant p53 proteins (mutp53) which can exhibit neomorphic, gain-of-function properties. The latter transform p53 into an oncoprotein that promotes metastatic tumor progression via downstream effectors such as ENTPD5, an endoplasmic reticulum UDPase involved in the calnexin/calreticulin cycle of N-glycoprotein biosynthesis. Elucidating the mechanisms underlying the pro-metastatic functions of the mutp53-ENTPD5 axis is crucial for developing targeted therapies for aggressive metastatic cancer.

Methods

We analyzed pancreatic, lung, and breast adenocarcinoma cells with p53 missense mutations to study the impact of mutp53 and ENTPD5 on the N-glycoproteins integrin-α5 (ITGA5) and integrin-β1 (ITGB1), which heterodimerize to form the key fibronectin receptor. We assessed the role of the mutp53-ENTPD5 axis in integrin-dependent tumor-stroma interactions and tumor cell motility using adhesion, migration, and invasion assays, identifying and validating therapeutic intervention targets. We employed an orthotopic xenograft model of pancreatic ductal adenocarcinoma to examine in vivo targeting of mutp53-ENTPD5-mediated ITGA5 regulation for cancer therapy.

Results

Mutp53 depletion diminished ITGA5 and ITGB1 expression and impaired tumor cell adhesion, migration, and invasion, rescued by ENTPD5. The mutp53-ENTPD5 axis maintained ITGA5 expression and function via the calnexin/calreticulin cycle. Targeting this axis using ITGA5-blocking antibodies, α-glucosidase inhibitors, or pharmacological degradation of mutp53 by HSP90 inhibitors, such as Ganetespib, effectively inhibited ITGA5-mediated cancer cell motility in vitro. In the orthotopic xenograft model, Ganetespib reduced ITGA5 expression and metastasis in an ENTPD5-dependent manner.

Conclusions

The mutp53-ENTPD5 axis fosters ITGA5 and ITGB1 expression and tumor cell motility through the calnexin/calreticulin cycle, contributing to cancer metastasis. ITGA5-blocking antibodies or α-glucosidase inhibitors target this axis and represent potential therapeutic options worth exploring in preclinical models. The pharmacologic degradation of mutp53 by HSP90 inhibitors effectively blocks ENTPD5-ITGA5-mediated cancer cell motility and metastasis in vivo, warranting further clinical evaluation in p53-mutant cancers. This research underscores the significance of understanding the complex interplay between mutp53, ENTPD5, and the calnexin/calreticulin cycle in integrin-mediated metastatic tumor progression, offering valuable insights for the development of potential therapeutic strategies.
Fance deficiency inhibits primordial germ cell proliferation associated with transcription–replication conflicts accumulate and DNA repair defects
Authors: Zhou Zhixian , Yin Huan , Suye Suye , Ren Zhen , Yan Lei , Shi Liye , Fu Chun
Published: August 2023

Fanconi anemia (FA) gene mutations are critical components in the genetic etiology of premature ovarian insufficiency (POI). Fance−/− mice detected meiotic arrest of primordial germ cells (PGCs) as early as embryonic day (E) 13.5 and exhibited decreased ovarian reserve after birth. However, the mechanism of Fance defect leading to dysgenesis of PGCs is unclear. We aimed to explore the effect of Fance defects on mitotic proliferation of PGCs. Combined with transcriptomic sequencing and validation, we examined the effect of Fance defects on cell cycle, transcription–replication conflicts (TRCs), and multiple DNA repair pathways in PGCs during active DNA replication at E11.5 and E12.5. Results showed Fance defects cause decreased numbers of PGCs during rapid mitosis at E11.5 and E12.5. Mitotic cell cycle progression of Fance−/− PGCs was blocked at E11.5 and E12.5, shown by decreased cell proportions in S and G2 phases and increased cell proportions in M phase. RNA-seq suggested the mechanisms involved in DNA replication and repair. We found Fance−/− PGCs accumulate TRCs during active DNA replication at E11.5 and E12.5. Fance−/− PGCs down-regulate multiple DNA repair pathways at E11.5 and E12.5 including the FA pathway, homologous recombination (HR) pathway, and base excision repair (BER) pathway. In conclusion, Fance defect impaired the mitotic proliferation of PGCs leading to rapidly decreased numbers and abnormal cell cycle distribution. Proliferation inhibition of Fance−/− PGCs was associated with accumulated TRCs and down-regulation of FA, HR, BER pathways. These provided a theoretical basis for identifying the inherited etiology and guiding potential fertility management for POI.
CCR2 macrophage response determines the functional outcome following cardiomyocyte transplantation
Authors: Vasudevan Praveen , Wolfien Markus , Lemcke Heiko , Lang Cajetan Immanuel , Skorska Anna , Gaebel Ralf , Galow Anne-Marie , Koczan Dirk , Lindner Tobias , Bergmann Wendy , Mueller-Hilke Brigitte , Vollmar Brigitte , Krause Bernd Joachim , Wolkenhauer Olaf , Steinhoff Gustav , David Robert
Published: August 2023

Background

The immune response is a crucial factor for mediating the benefit of cardiac cell therapies. Our previous research showed that cardiomyocyte transplantation alters the cardiac immune response and, when combined with short-term pharmacological CCR2 inhibition, resulted in diminished functional benefit. However, the specific role of innate immune cells, especially CCR2 macrophages on the outcome of cardiomyocyte transplantation, is unclear.

Methods

We compared the cellular, molecular, and functional outcome following cardiomyocyte transplantation in wildtype and T cell- and B cell-deficient Rag2del mice. The cardiac inflammatory response was assessed using flow cytometry. Gene expression profile was assessed using single-cell and bulk RNA sequencing. Cardiac function and morphology were determined using magnetic resonance tomography and immunohistochemistry respectively.

Results

Compared to wildtype mice, Rag2del mice show an increased innate immune response at steady state and disparate macrophage response after MI. Subsequent single-cell analyses after MI showed differences in macrophage development and a lower prevalence of CCR2 expressing macrophages. Cardiomyocyte transplantation increased NK cells and monocytes, while reducing CCR2−MHC-IIlo macrophages. Consequently, it led to increased mRNA levels of genes involved in extracellular remodelling, poor graft survival, and no functional improvement. Using machine learning-based feature selection, Mfge8 and Ccl7 were identified as the primary targets underlying these effects in the heart.

Conclusions

Our results demonstrate that the improved functional outcome following cardiomyocyte transplantation is dependent on a specific CCR2 macrophage response. This work highlights the need to study the role of the immune response for cardiomyocyte cell therapy for successful clinical translation.
Effect of intra-knee injection of autologous adipose stem cells or mesenchymal vascular components on short-term outcomes in patients with knee osteoarthritis: an updated meta-analysis of randomized controlled trials
Authors: Yang Yang , Lan Zhibin , Yan Jiangbo , Tang Zhiqun , Zhou Linghui , Jin Dian , Jin Qunhua
Published: August 2023

Objective

Assess the efficacy of single and multiple intra-articular injections of autologous adipose-derived stem cells (ASCs) and adipose-derived stromal vascular fraction (ADSVF) for the treatment of knee osteoarthritis (OA).

Methods

We conducted a thorough and systematic search of several databases, including PubMed, Embase, Web of Science, Cochrane Library, and ClinicalTrials.gov, to identify relevant studies. The included studies were randomized controlled trials (RCTs) that involved single or multiple intra-articular injections of autologous ASCs or ADSVF for the treatment of patients with knee osteoarthritis, without any additional treatment, and compared to either placebo or hyaluronic acid.

Results

A total of seven RCTs were analyzed in this study. The results of the meta-analysis show that compared to the control group, both single and multiple intra-articular injections of ASCs or ADSVF demonstrated superior pain relief in the short term (Z = 3.10; P < 0.0001 and Z = 4.66; P < 0.00001) and significantly improved function (Z = 2.61; P < 0.009 and Z = 2.80; P = 0.005). Furthermore, MRI assessment showed a significant improvement in cartilage condition compared to the control group. (Z = 8.14; P < 0.000001 and Z = 5.58; P < 0.00001).

Conclusions

In conclusion, in osteoarthritis of the knee, single or multiple intra-articular injections of autologous ASCs or ADSVF have shown significant pain improvement and safety in the short term in the absence of adjuvant therapy. Significant improvements in cartilage status were also shown. A larger sample size of randomized controlled trials is needed for direct comparison of the difference in effect between single and multiple injections.
Whole genome sequence of Citrus yellow vein clearing virus CA1 isolate
Authors: Sun Yongduo , Yokomi Raymond
Published: August 2023

Objectives

Citrus yellow vein clearing virus (CYVCV) is an emerging disease that poses a significant threat to the citrus industry in California. In this study, the viral genomic RNA was isolated from Eureka lemon plants in the greenhouse exhibiting CYVCV symptoms. Subsequently, the corresponding DNA genome amplicon was sequenced and annotated. These efforts expand the genotype database of CYVCV, which aims to enhance detection assays, promote understanding of the virus’s genetics and evolution, and support the management of this disease.

Data description

In this report, we present the complete genome sequence of the CYVCV California isolate (CA1). The genome was found to be 7,530 bp in length, with a G + C content of 51.7%. The 5′ and 3′ termini were determined using 5′ and 3′ termini rapid amplification of cDNA ends (RACE) systems. Furthermore, our analysis revealed the presence of six open reading frames (ORFs) potentially encoding proteins. All sequence data and annotation have been deposited in GenBank under the accession number OR037276.1.
Acute liver failure due to herpes simplex viral hepatitis diagnosed by skin lesions and blood tests: a case report
Authors: Shionoya Kento , Kako Makoto , Masuda Sakue , Makazu Makomo , Koizumi Kazuya
Published: August 2023

Background

The incidence of acute liver failure from herpes simplex virus is rare.

Case presentation

A 71-year-old Japanese man was diagnosed with acute liver failure and was transferred to our hospital. Steroid therapy, plasma exchange, and hemodiafiltration were started for liver failure, and antimicrobial therapy was initiated for pneumonia. Staphylococcus epidermidis was detected in blood culture. Skin rash appeared; a positive anti-herpes simplex virus result led to the diagnosis of acute liver failure from herpes simplex virus. Hence, acyclovir was started. After blood tests improved, treatments for acute liver failure were discontinued. Antimicrobial therapy was continued; however, he died. In this case, persistent bacteremia and drug-induced liver damage due to acyclovir may have contributed to his death.

Conclusions

Acute liver failure can lead to complications and death. Thus, careful observation is crucial, even if the patient has shown some improvements.
Cinnamic acid and p-coumaric acid are metabolized to 4-hydroxybenzoic acid by Yarrowia lipolytica
Authors: Konzock Oliver , Tous-Mohedano Marta , Cibin Irene , Chen Yun , Norbeck Joakim
Published: August 2023

Yarrowia lipolytica has been explored as a potential production host for flavonoid synthesis due to its high tolerance to aromatic acids and ability to supply malonyl-CoA. However, little is known about its ability to consume the precursors cinnamic and p-coumaric acid. In this study, we demonstrate that Y. lipolytica can consume these precursors through multiple pathways that are partially dependent on the cultivation medium. By monitoring the aromatic acid concentrations over time, we found that cinnamic acid is converted to p-coumaric acid. We identified potential proteins with a trans-cinnamate 4-monooxygenase activity in Y. lipolytica and constructed a collection of 15 knock-out strains to identify the genes responsible for the reaction. We identified YALI1_B28430g as the gene encoding for a protein that converts cinnamic acid to p-coumaric acid (designated as TCM1). By comparing different media compositions we found that complex media components (casamino acids and yeast extract) induce this pathway. Additionally, we discover the conversion of p-coumaric acid to 4-hydroxybenzoic acid. Our findings provide new insight into the metabolic capabilities of Y. lipolytica and hold great potential for the future development of improved strains for flavonoid production.
Melasma in people with darker skin types: a scoping review protocol on prevalence, treatment options for melasma and impact on quality of life
Authors: Mpofana Nomakhosi , Chibi Buyisile , Gqaleni Nceba , Hussein Ahmed , Finlayson Avenal Jane , Kgarosi Kabelo , Dlova Ncoza Cordelia
Published: August 2023

Background

Melasma is one of the most encountered dermatoses in dermatology and skin care clinics. It is a challenging chronic, recurrent condition associated with hyperpigmentation. Its aetiology is poorly understood. Melasma affects all races and gender but is more prevalent in women with darker skin types. Being a facial lesion, melasma has a severe impact on quality of life due to its disfigurement. While many modalities of treatment for melasma exists, unfortunately, effectiveness and safety remain a huge concern. Treatment modalities are variable and often unsatisfactory. The objective of this scoping review is to systemically map available evidence from literature regarding melasma on people with darker skin types, garner insight as to how melasma affects the quality of life and begin to investigate and gain understanding on effectiveness of different treatments used for melasma.

Methods

A scoping review guided by Arksey and O’Malley’s framework, the enhancements and recommendations of Levac, Colquhoun and O’Brien, Daudt and associates and the 2015 Johanna Briggs Institute’s guidelines will be conducted. Systematic electronic searches of databases and search engines will include Scopus, PubMed, CINAHL Complete, Cochrane, Science Direct, and Web of Science which will be conducted to attain published peer-reviewed articles of all study designs excluding reviews and grey literature. All literature that meets the inclusion criteria, research question and sub-question will be included in this review. All the retrieved literature will be exported to an Endnote X20 library. Quality appraisal of the included articles will be conducted using the mixed methods appraisal tool (MMAT) 2018 version.

Discussion

We anticipate mapping relevant literature on the melasma, investigating the effectiveness of treatment options of melasma as well as evaluating its association with quality of life in people with darker skin types. This study is likely to reveal research gaps, which could guide future implementation research on melasma treatment interventions.

Systematic review registration

This protocol has been registered a priori with OSF and is accessible on this link: https://osf.io/ru3jc/.
Inequalities for partial determinants of accretive block matrices
Authors: Fu Xiaohui , Hu Lihong , Salarzay Abdul Haseeb
Published: August 2023

Let $A=[A_{i,j}]^{m}_{i,j=1}\in \mathbf{M}_{m}(\mathbf{M}_{n})$
A
=

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,
j

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,
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=
1

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∈

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(

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be an accretive block matrix. We write det1 and det2 for the first and second partial determinants, respectively. In this paper, we show that $$\begin{aligned} \bigl\Vert \operatorname{det}_{1} (\operatorname{Re}A) \bigr\Vert \leq \biggl\Vert \biggl(\frac{\operatorname{tr}( \vert A \vert )}{m} \biggr)^{m}I_{n} \biggr\Vert \end{aligned}$$

∥

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(
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A
)
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≤

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(

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(
|
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)

m

)

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n

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and $$\begin{aligned} \bigl\Vert \operatorname{det}_{2} (\operatorname{Re}A) \bigr\Vert \leq \biggl\Vert \biggl( \frac {\operatorname{tr}( \vert A \vert )}{n} \biggr)^{n}I_{m} \biggr\Vert \end{aligned}$$

∥

det
2

(
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hold for any unitarily invariant norm $\|\cdot \|$
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⋅
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. The two inequalities generalize some known results related to partial determinants of positive-semidefinite block matrices.
Two-dimensional materials for high density, safe and robust metal anodes batteries
Authors: Wong Hoilun , Li Yuyin , Wang Jun , Tang Tsz Wing , Cai Yuting , Xu Mengyang , Li Hongliang , Kim Tae-Hyung , Luo Zhengtang
Published: August 2023

With a high specific capacity and low electrochemical potentials, metal anode batteries that use lithium, sodium and zinc metal anodes, have gained great research interest in recent years, as a potential candidate for high-energy-density storage systems. However, the uncontainable dendrite growth during the repeated charging process, deteriorates the battery performance, reduces the battery life and more importantly, raises safety concerns. With their unique properties, two-dimensional (2D) materials, can be used to modify various components in metal batteries, eventually mitigating the dendrite growth, enhancing the cycling stability and rate capability, thus leading to safe and robust metal anodes. In this paper, we review the recent advances of 2D materials and summarize current research progress of using 2D materials in the applications of (i) anode design, (ii) separator engineering, and (iii) electrolyte modifications by guiding metal ion nucleation, increasing ion conductivity, homogenizing the electric field and ion flux, and enhancing the mechanical strength for safe metal anodes. The 2D material modifications provide the ultimate solution for obtaining dendrite-free metal anodes, realizes the high energy storage application, and indicates the importance of 2D materials development. Finally, in-depth understandings of subsequent metal growth are lacking due to research limitations, while more advanced characterizations are welcome for investigating the metal deposition mechanism. The more facile and simplified preparation of 2D materials possess great prospects in high energy density metal anode batteries, and thus fulfils the development of EVs.
Enhanced phosphate adsorption and desorption characteristics of MgO-modified biochars prepared via direct co-pyrolysis of MgO and raw materials
Authors: Tu Panfeng , Zhang Guanlin , Cen Yingyuan , Huang Baoyuan , Li Juan , Li Yongquan , Deng Lifang , Yuan Haoran
Published: August 2023

Biochar modified by metal ions—particularly Mg—is typically used for the effective recovery of phosphorous. In this study, MgO-modified biochars were synthesized via the direct co-pyrolysis of MgO and raw materials such as rice straw, corn straw, Camellia oleifera shells, and branches from garden waste, which were labeled as MRS, MCS, MOT, and MGW, respectively. The resulting phosphate (PO) adsorption capacities and potential adsorption mechanisms were analyzed. The PO adsorption capacities of the biochars were significantly improved after the modification with MgO: MRS (24.71 ± 0.32 mg/g) > MGW (23.55 ± 0.46 mg/g) > MOT (15.23 ± 0.19 mg/g) > MCS (14.12 ± 0.21 mg/g). PO adsorption on the modified biochars was controlled by physical adsorption, precipitation, and surface inner-sphere complexation processes, although no electrostatic attraction was observed. Furthermore, PO adsorbed on modified biochars could be released under acidic, alkaline, and neutral conditions. The desorption efficiency of MRS was modest, indicating its suitability as a slow-release fertilizer.

Graphical Abstract
Successful treatment of nonocclusive mesenteric ischemia in a reconstructed jejunum after esophagectomy and remnant gastric tube resection: a case report
Authors: Okamoto Koichi , Saito Hiroto , Shimada Mari , Yamaguchi Takahisa , Tsuji Toshikatsu , Moriyama Hideki , Kinoshita Jun , Nakamura Keishi , Ninomiya Itasu , Takamura Hiroyuki , Inaki Noriyuki
Published: August 2023

Background

Nonocclusive mesenteric ischemia (NOMI), an ischemic bowel disease without a disruption of the mesenteric blood flow or strangulation of the mesentery or intestine, may cause a lethal clinical course. We report a very rare case of jejunal necrosis caused by NOMI in the pedicled mesentery of the reconstructed jejunum after remnant gastric tube resection for heterochronous gastric tube cancer after esophagectomy.

Case presentation

An 80-year-old man visited our department with chief complaints of fever and appetite loss after 4 months from gastric tube resection and digestive reconstruction with pedicled jejunum. Contrast-enhanced computed tomography (CT) revealed impaired blood flow without torsion of the mesentery, severe wall thickness, and micro-penetration in the reconstructed jejunum and combined pyothorax in the right thoracic cavity. Esophagogastroduodenoscopy demonstrated extensive mucosal necrosis confined to the jejunum, which was elevated in the thoracic cavity. The jejunal necrosis due to NOMI occurring in the reconstructed jejunum was suspected, and lifesaving small bowel resection with right thoracotomy was considered necessary. However, radical operation with right thoracotomy was considered to be excessively invasive and not valid due to the patient’s poor physical status, advanced age, and presence of left adrenal metastasis from the remnant gastric cancer. Therefore, we selected the conservative treatment with fasting, transnasal drainage, and administration of antibiotics due to the patient’s intention. CT-guided right thoracic drainage for the intrathoracic abscess was needed 10 days after starting treatment and the inflammatory response rapidly improved. Follow-up CT and esophagogastroduodenoscopy revealed the improvement in the ischemic changes in jejunal mucosa without perforation. Intake was initiated at 20 days after symptom onset, and the patient was discharged at 40 hospital days without any complications and sequelae.

Conclusions

To the best of our knowledge, this is the first case of NOMI occurring in the reconstructed jejunum after remnant gastric tube resection that was successfully treated with a conservative treatment. For NOMI, it is important to make appropriate diagnosis based on imaging findings and perform proper assessment of the patient’s condition. Conservative treatments may be also useful depending on the patient’s condition.
A Systematic Review of the Physical, Physiological, Nutritional and Anthropometric Profiles of Soccer Referees
Authors: Martinho Diogo V. , Field Adam , Rebelo André , Gouveia Élvio R. , Sarmento Hugo
Published: August 2023

Background

The importance of soccer referees is widely accepted by international soccer organizations and governing bodies, although there is little research summarizing and appraising the literature on soccer referees. The aim of this study was to systematically review the information related to physical demands, nutrition and physiological profiling of soccer referees.

Methods

Conforming with Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, searches of three electronic databases (Web of Sciences, PubMed and Scopus) were conducted on 24 April 2022. The following search terms were used: (Soccer OR football AND refer*) AND (physical OR physiolo* OR load* OR “body composition” OR “fat mass” OR “fat free mass” OR “body size” OR “nutrition*” OR “nutritional assessment” OR “nutritional intake” OR “macronutrient*” OR “micronutrient*”). The inclusion criteria of the manuscripts written in English were that articles with male and/or female soccer referees and included relevant data concerning performance, physical testing, nutrition, body composition, body size and/or physiology of soccer referees. The tools developed by the National Institute of Health were used to assess risk of bias according to the study design.

Results

In total, 110 manuscripts were included in the present review. Match activities of soccer referees and assistant referees are not comparable. Variation in performance is influenced by competitive level and competitive schedules. Additionally, match performance is often used to validate field protocols. The associations between match indicators and field protocols were not constant across the included studies, particularly in short-maximal tests. An age decline in physical performance was not conclusive. Body size did not discriminate between referees and assistant referees, as well as referees of different competitive levels. Few studies focused on nutritional guidelines among referees, particularly exercise energy expenditure. Energy intake was comparable across studies, but referees did not follow the daily dietary recommendations. Aerobic output was frequently assessed, and it was not comparable to the values for soccer players.

Conclusions

Although there are decreases in the running profiles of older referees, they maintain the same distances from the ball, and thus, age per se should not be used to define participation at the international level. The assessment of physical fitness warrants future consideration given the levels of fatigue that are apparent at the end of matches. In order to attain ideal levels of body composition, future studies need to provide guidelines for daily energy expenditure and nutritional intake.

Trial registration: The protocol was published in INPLASY (International Platform of Registered Systematic Review and Meta-Analysis Protocols) with the registration number 202280052 and https://doi.org/10.37766/inplasy2022.8.005.
Associations Between Cancer Predisposition Mutations and Clonal Hematopoiesis in Patients With Solid Tumors
Authors: Franch-Expósito Sebastià , Mehine Miika , Ptashkin Ryan N. , Bolton Kelly L. , Bandlamudi Chaitanya , Srinivasan Preethi , Zhang Linda , Goodell Margaret A. , Gedvilaite Erika , Menghrajani Kamal , Sánchez-Vela Pablo , Mandelker Diana , Comen Elizabeth , Norton Larry , Benayed Ryma , Gao Teng , Papaemmanuil Elli , Taylor Barry , Levine Ross , Offit Kenneth , Stadler Zsofia , Berger Michael F. , Zehir Ahmet
Published: August 2023

PURPOSE

Clonal hematopoiesis (CH), the expansion of clones in the hematopoietic system, has been linked to different internal and external features such as aging, genetic ancestry, smoking, and oncologic treatment. However, the interplay between mutations in known cancer predisposition genes and CH has not been thoroughly examined in patients with solid tumors.

METHODS

We used prospective tumor-blood paired sequencing data from 46,906 patients who underwent Memorial Sloan Kettering-Integrated Mutation Profiling of Actionable Cancer Targets (MSK-IMPACT) testing to interrogate the associations between CH and rare pathogenic or likely pathogenic (P/LP) germline variants.

RESULTS

We observed an enrichment of CH-positive patients among those carrying P/LP germline mutations and identified a significant association between P/LP germline variants in ATM and CH. Germline and CH comutation patterns in ATM, TP53, and CHEK2 suggested biallelic inactivation as a potential mediator of clonal expansion. Moreover, we observed that CH- PPM1D mutations, similar to somatic tumor-associated PPM1D mutations, were depleted in patients with P/LP germline mutations in the DNA damage response (DDR) genes ATM, CHEK2, and TP53. Patients with solid tumors and harboring P/LP germline mutations, CH mutations, and mosaicism chromosomal alterations might be at an increased risk of developing secondary leukemia while germline variants in TP53 were identified as an independent risk factor (hazard ratio, 36; P < .001) for secondary leukemias.

CONCLUSION

Our results suggest a close relationship between inherited variants and CH mutations within the DDR genes in patients with solid tumors. Associations identified in this study might translate into enhanced clinical surveillance for CH and associated comorbidities in patients with cancer harboring these germline mutations.
Epic Solutions of the “Goethe Problem” in Lukács and Moretti
Authors: Geulen Eva
Published: August 2023

What made Goethe’s Faust so special to Georg Lukács? The contribution explores possible answers and examines the theoretical implications of the unique position Goethe’s Faust occupies in Lukács’s postwar writings. During his engagement Lukács modifies some of his most dearly held convictions. For example, his criticism of mixing genres disappears in his identification of Faust’s peculiar form as an “epic-dramatic form” said to approximate the elusive epical poem. But as such, it is also the paradoxical manifestation of a singular form without predecessors or followers. The essay concludes with a coda on the aftermath of Lukács’s Faust obsession in the work of the latter-day Marxist Franco Moretti.
A universal method for generating knockout mice in multiple genetic backgrounds using zygote electroporation
Authors: Tamari Tomohiro , Ikeda Yoshihisa , Morimoto Kento , Kobayashi Keiko , Mizuno-Iijima Saori , Ayabe Shinya , Kuno Akihiro , Mizuno Seiya , Yoshiki Atsushi
Published: August 2023

Genetically engineered mouse models are essential tools for understanding mammalian gene functions and disease pathogenesis. Genome editing allows the generation of these models in multiple inbred strains of mice without backcrossing. Zygote electroporation dramatically removed the barrier for introducing the CRISPR-Cas9 complex in terms of cost and labour. Here, we demonstrate that the generalised zygote electroporation method is also effective for generating knockout mice in multiple inbred strains. By combining in vitro fertilisation and electroporation, we obtained founders for knockout alleles in eight common inbred strains. Long-read sequencing analysis detected not only intended mutant alleles but also differences in read frequency of intended and unintended alleles among strains. Successful germline transmission of knockout alleles demonstrated that our approach can establish mutant mice targeting the same locus in multiple inbred strains for phenotyping analysis, contributing to reverse genetics and human disease research.
The cortical microtubules of Toxoplasma gondii underlie the helicity of parasite movement
Authors: Tengganu Isadonna F. , Arias Padilla Luisa F. , Munera Lopez Jonathan , Liu Jun , Brown Peter T. , Murray John M. , Hu Ke
Published: August 2023

Motility is essential for apicomplexan parasites to infect their hosts. In a three-dimensional (3D) environment, the apicomplexan parasite Toxoplasma gondii moves along a helical path. The cortical microtubules, which are ultra-stable and spirally arranged, have been considered to be a structure that guides the long-distance movement of the parasite. Here, we address the role of the cortical microtubules in parasite motility, invasion and egress by utilizing a previously generated mutant (dubbed ‘TKO’) in which these microtubules are destabilized in mature parasites. We found that the cortical microtubules in ∼80% of the non-dividing (i.e. daughter-free) TKO parasites are much shorter than normal. The extent of depolymerization was further exacerbated upon commencement of daughter formation or cold treatment, but parasite replication was not affected. In a 3D Matrigel matrix, the TKO mutant moved directionally over long distances, but along trajectories that were significantly more linear (i.e. less helical) than those of wild-type parasites. Interestingly, this change in trajectory did not impact either movement speed in the matrix or the speed and behavior of the parasite during entry into and egress from the host cell.
Modern approaches to address the concept of territorial division in Islamic jurisprudence
Authors: Alotaibi Hajed A.
Published: August 2023

Background: This article examines how current jurists can deal with a juristic division of the world into abode of "Islam” and “infidelity". The success with which jurists re-evaluate this dichotomy will have major impacts on how an Islamic state interacts with non-Muslim governments on the one hand, and Muslims residing temporarily or permanently in a non-Muslim territory on the other.

Methods: Here, an attempt made to review the concepts of Dar Al-Islam and Dar Al-Harb from two perspectives: that of the dialectical limits and liberalization, as discussed in secondary material such as books, research articles, and reports.

Results: this paper has explored and discussed the criteria for identifying Dar Al-Islam and Dar Al-Harb in Islamic jurisprudence.

Conclusions: This article analyzes the various arguments put forward to establish new ways of peaceful coexisting and maintaining healthy international connections.
Cointegration and causality relationship of Indian stock market with selected world markets
Authors: Ali Farman , Suri Pradeep , Kaur Tarunpreet , Bisht Deepa
Published: August 2023

Background: The purpose of this study is to explore the trends and causes of established and emerging nations' stock market integration with India. The National Stock Exchange (NSE) indices act as a counterweight to international market indices.

This study investigates the sustained interest of foreign investors in the Indian stock market in the wake of capital market reforms, as well as whether it moves in tandem with other markets in Asia and the United States.

Methods: Our study examined the possibility of cross-country cointegration between the largest economies and indices around the world using multiple financial econometric models, such as Augmented Dickey-Fuller, Unit Root, Correlation, and Johansen Cointegration.

Results: The findings of this study significantly support the notion that Indian and international financial markets are highly integrated. Vector error correction model indicates that the Indian market (NSE) is highly cointegrated with the US market (National Association of Securities Dealers Automated Quotations) and increased volatility signifies global contagion.

Conclusion: A cursory examination of the data reveals distinct investment and portfolio diversification options for global investors. This could assist regulators in formulating more effective rules regarding price discovery processes.