bluuebunny/crossref_metadata_2025 huggingface dataset:
The Synthesis and Characterization of a Delivery System Based on Polymersomes and a Xanthone with Inhibitory Activity in Glioblastoma
Authors: Alves Ana , Silva Ana Margarida , Nunes Claúdia , Cravo Sara , Reis Salette , Pinto Madalena , Sousa Emília , Rodrigues Francisca , Ferreira Domingos , Costa Paulo C. , Correia-da-Silva Marta
Published: January 2024

Glioblastoma (GBM) is the most common and deadly primary malignant brain tumor. Current therapies are insufficient, and survival for individuals diagnosed with GBM is limited to a few months. New GBM treatments are urgent. Polymeric nanoparticles (PNs) can increase the circulation time of a drug in the brain capillaries. Polymersomes (PMs) are PNs that have been described as having attractive characteristics, mainly due to their stability, prolonged circulation period, biodegradability, their ability to sustain the release of drugs, and the possibility of surface functionalization. In this work, a poly(ethylene glycol)-ε-caprolactone (PEG-PCL) copolymer was synthesized and PMs were prepared and loaded with an hydrolytic instable compound, previously synthesized by our research team, the 3,6-bis(2,3,4,6-tetra-O-acetyl-β-glucopyranosyl)xanthone (XGAc), with promising cytotoxicity on glioblastoma cells (U-373 MG) but also on healthy cerebral endothelial cells (hCMEC/D3). The prepared PMs were spherical particles with uniform morphology and similar sizes (mean diameter of 200 nm) and were stable in aqueous suspension. The encapsulation of XGAc in PMs (80% encapsulation efficacy) protected the healthy endothelial cells from the cytotoxic effects of this compound, while maintaining cytotoxicity for the glioblastoma cell line U-373 MG. Our studies also showed that the prepared PMs can efficiently release XGAc at intratumoral pHs.
Study on the Performance Mechanism of Polyformaldehyde Glycol Ether Polymer for Crude Oil Recovery Enhancement
Authors: Jiang Shaohui , Lu Wenxue , Li Tao , Ma Fujun , Yao Dahu , Li Qingsong
Published: January 2024

The demand for energy continues to increase as the global economy continues to grow. The role of oilfield chemicals in the process of oil and gas exploration, development, and production is becoming more and more important, and the demand is rising year by year. The support of national policies and the formulation of environmental protection regulations have put forward higher requirements for oilfield chemical products, which has promoted the innovative research and development and market application of oilfield chemicals. Polyformaldehyde glycol ether polymer (PGEP) is simple to synthesize, easily biodegradable, green and environmentally friendly, and in line with the development trend of chemicals used in oil and gas development. The interfacial tension performance of PGEP after compounding with different surfactants can reach as low as 0.00034 mN/m, which meets the requirements of the oilfield (interfacial tension ≤ 5 × 10−3 mN/m). The best oil washing efficiency performance of PGEP compounded with different surfactants reached 78.2%, which meets the requirements of the oilfield (oil washing efficiency ≥ 40%). The fracturing fluid drainage efficiency of PGEP after compounding with different surfactants reaches 22%, which meets the requirements of the oilfield (drainage efficiency ≥ 15%). The surface interfacial tension of the system remains constant after the concentration exceeds 0.2% and decreases with lower concentrations. The drainage efficiency increases with increasing concentrations in the range below 0.6%. It was determined that PGEP can be used as a surfactant instead of fatty-alcohol ethoxylates (FAE) in oilfield development.
Zinc Complexes of Fluorosubstituted N-[2-(Phenyliminomethyl)phenyl]-4-methylbenzenesulfamides: Synthesis, Structure, Luminescent Properties, and Biological Activity
Authors: Burlov Anatolii S. , Vlasenko Valery G. , Milutka Maxim S. , Koshchienko Yurii V. , Lazarenko Vladimir A. , Trigub Alexander L. , Kolodina Alexandra A. , Zubenko Alexander A. , Braga Elena V. , Gusev Alexey N. , Linert Wolfgang
Published: January 2024

Mono-, di-, and trifluorophenyl substituted in different positions of amine fragments bis [2-[[(E)-((fluorophenyl)iminomethyl]-N-(p-tolylsulfonyl)anilino]zinc(II) complexes were synthesized. Their crystal structure, photo- and electroluminescent properties, and protistocidal, fungistatic, and antibacterial activities were studied. It has been shown that the introduction of fluorine atoms and an increase in their number in the ligand structure of the resulting metal complexes promote the luminescence quantum yields and values of performance and brightness in EL cells compared to their previously studied chlorine-substituted analogs.
Micro-Scale Deformation Aspects of Additively Fabricated Stainless Steel 316L under Compression
Authors: Kurdi Abdulaziz , Degnah Ahmed , Tabbakh Thamer , Alnaser Husain , Basak Animesh Kumar
Published: January 2024

The deformation aspects associated with the micro-mechanical properties of the powder laser bed fusion (P-LBF) additively manufactured stainless steel 316L were investigated in the present work. Toward that, micro-pillars were fabricated on different planes of the stainless steel 316L specimen with respect to build direction, and an in situ compression was carried out inside the chamber of the scanning electron microscope (SEM). The results were compared against the compositionally similar stainless steel 316L, which was fabricated by a conventional method, that is, casting. The post-deformed micro-pillars on the both materials were examined by electron microscopy. The P-LBF processed steel exhibits equiaxed as well as elongated grains of different orientation with the characteristics of the melt-pool type arrangements. In contrast, the cast alloy shows typical circular-type grains in the presence of micro-twins. The yield stress and ultimate compressive stress of P-LBF fabricated steel were about 431.02 ± 15.51 − 474.44 ± 23.49 MPa and 547.78 ± 29.58 − 682.59 ± 21.59 MPa, respectively. Whereas for the cast alloy, it was about 322.38 ± 19.78 MPa and 477.11 ± 25.31 MPa, respectively. Thus, the outcome of this study signifies that the AM-processed samples possess higher mechanical properties than conventionally processed alloy of similar composition. Irrespective of the processing method, both specimens exhibit ductile-type deformation, which is typical for metallic alloys.
Investigations on the Surface Integrity and Wear Mechanisms of TiAlYN-Coated Tools in Inconel 718 Milling Operations
Authors: Silva Francisco J. G. , Sebbe Naiara P. V. , Costa Rúben D. F. S. , Pedroso André F. V. , Sales-Contini Rita C. M. , Barbosa Marta L. S. , Martinho Rui P.
Published: January 2024

Inconel 718 is a Ni superalloy with superior mechanical properties, even at high temperatures. However, due to its high hardness and low thermal conductivity, it is considered a difficult-to-machine material. This material is widely used in applications that require good dimensional stability, making the milling process the most used in machining this alloy. The wear resulting from this process and the quality of the machined surface are still challenging factors when it comes to Inconel 718. TiAlN-based coating has been used on cutting tools with Yttrium as a doping element to improve the process performance. Based on this, this work evaluated the machined surface integrity and wear resistance of cutting tools coated using Physical Vapor Deposition (PVD) HiPIMS with TiAlYN in the end milling of Inconel 718, varying the process parameters such as cutting speed (vc), feed per tooth (fz), and cutting length (Lcut). It was verified that the Lcut is the parameter that exerts the most significant influence since, even at small distances, Inconel 718 already generates high tool wear (TW). Furthermore, the main wear mechanisms were abrasive and adhesive wear, with the development of a built-up edge (BUE) under a125 m/min feed rate (f) and a Lcut = 15 m. Chipping, cracking, and delamination of the coating were also observed, indicating a lack of adhesion between the coating and the substrate, suggesting the need for a good interlayer or the adjustment of the PVD parameters.
Study on the Compressive and Flexural Properties of Coconut Fiber Magnesium Phosphate Cement Curing at Different Low Temperatures
Authors: Lin Zhiwei , Zhang Liwen , Zheng Wenzhi , Huang Xiangyun , Zhang Junping
Published: January 2024

The incorporation of coconut fiber (CF) into magnesium phosphate cement (MPC) can effectively improve upon its high brittleness and ease of cracking. In practical engineering, coconut fiber-reinforced magnesium phosphate cement (CF-MPC) will likely work in cold environments. Therefore, it is essential to understand the effects of various types of low-temperature curing on CF-MPC performances, but there are very few studies in this area. In this study, the static compression and three-point bending test were utilized to examine the compressive and flexural characteristics of CF-MPC with various CF contents and different negative curing temperatures. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) were conducted to observe the impact of low-temperature maintenance on the structure and hydration reaction of the specimens. The results indicate that CF-MPC curing at low temperatures was more prone to cracks during compression and bending, while the appropriate amount of CF could enhance its plastic deformation capability. The CF-MPC’s compressive and flexural strength declined as the curing temperature dropped. Moreover, with the rise in CF content, the samples’ compressive strength also tended to fall, and there was a critical point for the change in flexural strength. In addition, MPC’s primary hydration product (MgKPO4·6H2O) decreased with a drop in curing temperature, and more holes and fractures appeared in CF-MPC.
Effect of Silver Powder Microstructure on the Performance of Silver Powder and Front-Side Solar Silver Paste
Authors: Yu Xianglei , Sun Hu , Qian Zhuo , Li Weichao , Li Wei , Huang Fuchun , Li Junpeng , Gan Guoyou
Published: January 2024

Silver powder, as the primary component of solar silver paste, significantly influences various aspects of the paste’s performance, including printing, sintering, and conductivity. This study reveals that, beyond the shape and size of the silver powders, their microstructure is a critical factor influencing the performance of both silver powders and silver pastes in solar cell applications. The growth process leads to the formation of either polycrystalline aggregated silver powder or crystal growth silver powder. Analyzing the performance characteristics of these different microstructures provides guidance for selecting silver powders for silver pastes at different sintering temperatures. Polycrystalline aggregated silver powder exhibits higher sintering activity, with a sintering initiation temperature around 450 °C. The resulting silver paste, sintered at 750 °C, demonstrates a low sheet resistance of 2.92 mΩ/sq and high adhesion of 2.13 N. This silver powder is suitable for formulating silver pastes with lower sintering temperatures. The solar cell electrode grid lines have a high aspect ratio of 0.37, showing poor uniformity. However, due to the high sintering activity of the silver powder, the glass layer dissolves and deposits more silver, resulting in excellent conductivity, a low contact resistance of the silver electrode, a low series resistance of the solar cell of 1.23 mΩ, and a high photoelectric conversion efficiency of 23.16%. Crystal growth silver powder exhibits the highest tap density of 5.52 g/cm3. The corresponding silver paste shows improved densification upon sintering, especially at 840 °C, yielding a sheet resistance of 2.56 mΩ/sq and adhesion of 3.05 N. This silver powder is suitable for formulating silver pastes with higher sintering temperatures. The solar cell electrode grid lines are uniform with the highest aspect ratio of 0.40, resulting in a smaller shading area, a high fill factor of 81.59%, and a slightly higher photoelectric conversion efficiency of 23.17% compared to the polycrystalline aggregated silver powder.
A Review of Modeling of Composite Structures
Authors: Yu Wenbin
Published: January 2024

This paper provides a brief review on modeling of composite structures. Composite structures in this paper refer to any structure featuring anisotropy and heterogeneity, including but not limited to their traditional meaning of composite laminates made of unidirectional fiber-reinforced composites. Common methods used in modeling of composite structures, including the axiomatic method, the formal asymptotic method, and the variational asymptotic method, are illustrated in deriving the classical lamination theory for the composite laminated plates. Future research directions for modeling composite structures are also pointed out.
A Novel Technique for Substrate Toughening in Wood Single Lap Joints Using a Zero-Thickness Bio-Adhesive
Authors: Jalali Shahin , Borges Catarina da Silva Pereira , Carbas Ricardo João Camilo , Marques Eduardo André de Sousa , Akhavan-Safar Alireza , Barbosa Ana Sofia Oliveira Ferreira , Bordado João Carlos Moura , da Silva Lucas Filipe Martins
Published: January 2024

In contemporary engineering practices, the utilization of sustainable materials and eco-friendly techniques has gained significant importance. Wooden joints, particularly those created with polyurethan-based bio-adhesives, have garnered significant attention owing to their intrinsic environmental advantages and desirable mechanical properties. In comparison to conventional joining methods, adhesive joints offer distinct benefits such as an enhanced load distribution, reduced stress concentration, and improved aesthetic appeal. In this study, reference and toughened single-lap joint samples were investigated experimentally and numerically under quasi-static loading conditions. The proposed research methodology involves the infusion of a bio-adhesive into the wooden substrate, reinforcing the matrix of its surfaces. This innovative approach was developed to explore a synergetic effect of both wood and bio-adhesive. The experimentally validated results showcase a significant enhancement in joint strength, demonstrating an 85% increase when compared to joints with regular pine substrates. Moreover, the increased delamination thickness observed in toughened joints was found to increase the energy absorption of the joint.
Microporous Fluorescent Poly(D,L-lactide) Acid–Carbon Nanodot Scaffolds for Bone Tissue Engineering Applications
Authors: Mauro Nicolò , Calabrese Giovanna , Sciortino Alice , Rizzo Maria G. , Messina Fabrizio , Giammona Gaetano , Cavallaro Gennara
Published: January 2024

In this study, we introduce novel microporous poly(D,L-lactide) acid–carbon nanodot (PLA-CD) nanocomposite scaffolds tailored for potential applications in image-guided bone regeneration. Our primary objective was to investigate concentration-dependent structural variations and their relevance to cell growth, crucial aspects in bone regeneration. The methods employed included comprehensive characterization techniques such as DSC/TGA, FTIR, rheological, and degradation assessments, providing insights into the scaffolds’ thermoplastic behavior, microstructure, and stability over time. Notably, the PLA-CD scaffolds exhibited distinct self-fluorescence, which persisted after 21 days of incubation, allowing detailed visualization in various multicolor modalities. Biocompatibility assessments were conducted by analyzing human adipose-derived stem cell (hADSC) growth on PLA-CD scaffolds, with results substantiated through cell viability and morphological analyses. hADSCs reached a cell viability of 125% and penetrated throughout the scaffold after 21 days of incubation. These findings underscore the scaffolds’ potential in bone regeneration and fluorescence imaging. The multifunctional nature of the PLA-CD nanocomposite, integrating diagnostic capabilities with tunable properties, positions it as a promising candidate for advancing bone tissue engineering. Our study not only highlights key aspects of the investigation but also underscores the scaffolds’ specific application in bone regeneration, providing a foundation for further research and optimization in this critical biomedical field.
Study of Lightweight Ceramic Matrix-Less Syntactic Foam Composed of Cenosphere Using Spark Plasma Sintering
Authors: Eiduks Toms Valdemars , Drunka Reinis , Abramovskis Vitalijs , Zalite Ilmars , Gavrilovs Pavels , Baronins Janis , Lapkovskis Vjaceslavs
Published: January 2024

The current investigation presents porous ceramic materials prepared with cenospheres (CS) by using spark plasma sintering. The impact of sintering temperature, mould diameter (20, 30 and 50 mm) and cenosphere size on the properties of the sintered material was investigated. Shrinkage of the samples during sintering started at 900 °C. Total sample shrinkage during sintering increases with increasing temperature and decreases with increasing mould size; increasing sample sintering temperature increases the apparent density of all sample series CS 63–150 µm in a 20 mm mould from 0.97 to 2.3 g·cm−3 at 1050–1300 °C; in a 30 mm mould, 0.81–1.87 g·cm−3 at 1050–1200 °C; in 50 mm mould, 0.54–0.75 g·cm−3 at 1050–1150 °C; while CS 150–250 µm in a 20 mm mould is 0.93–1.96 g·cm−3 at 1050–1200 °C. Total porosity decreases from 61.5% to 3.9% by increasing sintering temperature from 1050 to 1250 °C, while open porosity reduces at lower temperatures, with closed porosity being highest in samples sintered at 1150 °C. When the sintering temperature increases from 1050 to 1300 °C, the compressive strength of the CS 63–150 samples produced in a 20 mm mould increases from 11 MPa to 312 MPa. These results correlate with the Rice model, which describes an exponential dependence of compressive strength on material porosity and fully dense material compressive strength.
Gingival Manifestations in Oral Chronic Autoimmune Bullous Diseases: A Retrospective Study
Authors: Parlatescu Ioanina , Tovaru Serban , Tofan Cristina , Perlea Paula , Milanesi Elena , Dobre Maria , Mihai Laurenta Lelia
Published: January 2024

Background and Objectives: Desquamative gingivitis (DG) is a clinical term indicating “peeling gums” and is associated with different oral manifestations. In this study, we aimed to assess the association between DG and autoimmune blistering mucocutaneous diseases (ABMD) with oral manifestations. Materials and Methods: A retrospective study including 88 patients diagnosed between 1998 and 2019 with ABMD (intraepithelial and subepithelial autoimmune blistering diseases) was performed at the Oral Medicine Department, Faculty of Dentistry, “Carol Davila” University of Medicine and Pharmacy in Bucharest. For each patient, the sociodemographic and anamnestic data, as well as clinical features of oral lesions (location), histological evaluation, and direct immunofluorescence data were collected. Results: Most of the patients involved in the study were female (78.4%). In total, 34 patients (38.63%) were diagnosed with subepithelial autoimmune diseases (SAD) and 54 (61.36%) had intraepithelial autoimmune diseases (IAD). Differences in the anatomic distribution of oral involvement were found between SAD and IAD. The presence of DG was significantly more common in patients with SAD compared to those with a diagnosis of IAD. Conclusions: Specific anatomical locations of the oral lesions are significantly associated with different subtypes of ABMD, with gingiva and hard palate mucosa being more involved in SAD and the soft palate and buccal mucosa in IAD. Desquamative gingivitis is a clinical sign that raises diagnostic challenges for several conditions in oral medicine.
Transient Elastography Is the Best-Performing Non-Invasive Test of Liver Fibrosis in Obese Asian Patients with Non-Alcoholic Fatty Liver Disease: A Pilot, Cross-Sectional Study
Authors: Kalaiyarasi Kaliyaperumal , Sanchalika Acharyya , Hsien Min Low , Wei Ming Yap , Vishalkumar Shelat , Kuo Chao Yew , Jee Keem Low , Sameer Junnarkar , Terence Huey Cheong Wei , Yen Ping Tan
Published: January 2024

Background and Objectives: The prevalence of NAFLD (non-alcoholic fatty liver disease) is increasing, and up to 64% of Asian patients with NAFLD are obese. Non-invasive tests (NITs) for the assessment of liver fibrosis are increasingly being used, but data on their performance in obese Asian patients are lacking. In this pilot cross-sectional study, we aim to compare the distribution of serum and radiological markers of fibrosis between obese Asian biopsy-proven NAFLD patients with and without fibrosis and estimate the diagnostic accuracies of these indices. Materials and Methods: Obese Asian patients with NAFLD and who had undergone a liver biopsy showing histological evidence of NAFLD were invited to participate. Liver fibrosis was assessed using laboratory (APRI, AAR, BARD, FIB4, NFS, and Asia–Pacific NAFLD advanced fibrosis score) and imaging modalities (TE: transient elastography, MRE: magnetic resonance elastography, and SWU: shear wave ultrasonography). Results: A total of 16 patients were included in the final analysis. On liver biopsy, nine patients (56.3%) had significant fibrosis (F2 or higher), and six of these patients had advanced fibrosis (F3 or higher). F4 fibrosis was present in one patient (6.3%). For the laboratory markers, we found that the BARD score correctly identified five out of six patients with advanced fibrosis (83.4%, p value 0.045). Among all the NITs studied, liver stiffness measured by TE had the highest accuracy of 87.5% in its established threshold of 8.5 kPa for the detection of advanced fibrosis. MRE also performed well (81.2% in 3.64 kPa). Conclusions: In conclusion, TE has performed well in the detection of advanced fibrosis in obese Asian patients with biopsy-proven NAFLD in our pilot study. Further large-scale definitive studies are needed to validate the results of our findings.
A New Player in the Game: Can Exergame Be of Support in the Management of Atrial Fibrillation?
Authors: Leo Donato Giuseppe , Proietti Riccardo
Published: January 2024

Atrial fibrillation (AF) is the most common form of cardiac arrhythmia, currently affecting 2–3% of the world’s population. Traditional exercise and physical activity interventions have been successfully implemented in the management of AF, with the aim of improving patients’ quality of life and their exercise capacity, as well as reducing their mortality rate. Currently, new technology-mediated approaches to exercise, defined as exergame, have been shown to be successful in the delivery of exercise home-based interventions in patients with cardiovascular diseases. However, data on the effects of exergame on AF are not yet available. In this paper, we summarise the current literature on the role of traditional exercise in AF and how it affects the pathophysiology of this condition. We also review the current literature on exergame and its employment in cardiac rehabilitation and suggest its potential role in the management of AF patients. A review of the evidence suggests that traditional exercise (of light-to-moderate intensity) is beneficial in patients with AF. Additionally, exergame seems to be a promising approach for delivering exercise interventions in patients with cardiovascular diseases. Exergame may be a promising tool to improve the quality of life and exercise capacity in patients with AF, with the additional advantage of being remotely delivered, and the potential to increase patients’ engagement. Proper guidelines are required to prescribe exergame interventions, considering the principles of traditional exercise prescription and applying them to this new e-health approach. Further studies are needed to validate the use of exergame in patients with AF.
Corrosion Protection of Steel by NO3− and NO2− Intercalated Mg-Al Layered Double Hydroxides in Simulated Pore Solutions of Alkali-Activated Slag
Authors: Zhong Minxuan , Xu Jinxia , Jiang Yiyang , Wu You
Published: January 2024

This paper aims to perform a comparative investigation on the corrosion protection of steel in the simulated pore solutions of alkali-activated slag (SH) by NO3− and NO2− intercalated Mg-Al layered double hydroxides (MAL) which were fabricated by the calcination rehydration method. The corrosion potential, electrochemical impedance spectroscopy, potentiodynamic polarization and corrosion condition of steel were measured. Furthermore, changes in the microstructures of NO3− intercalated MAL (MAL-N3) and NO2− intercalated MAL (MAL-N2) before and after the adsorption of chloride ion were observed by X-ray diffraction and Fourier transform infrared spectroscopy. The results show that compared to the simulated concrete pore solution (OPCH), MAL-N3 and MAL-N2 exhibit lower chloride adsorption capacities and better corrosion inhibition effects in SH. The chloride adsorption capacity of MAL-N2 is lower compared with that of MAL-N3 due to the different volumes of intercalated anions. In contrast, MAL-N2 presents superior corrosion inhibition than MAL-N3. Furthermore, the decreases in [OH−] in SH due to the additions of MAL-N3 and MAL-N2 are more prominent than those in OPCH. The different synergistic effects due to the competitive anion-exchanges in the interlayers of NO3− and NO2− intercalated MAL in the two solutions contribute to the above effects.
Effect of Different Post-Processing Thermal Treatments on the Fracture Toughness and Tempering Resistance of Additively Manufactured H13 Hot-Work Tool Steel
Authors: Deirmina Faraz , Amirabdollahian Sasan , Pellizzari Massimo , Molinari Alberto
Published: January 2024

Near-full density and crack-free AISI H13 hot-work tool steel was fabricated using laser-directed energy deposition (L-DED). Two different heat-treatment scenarios, i.e., direct tempering (ABT) from the as-built (AB) condition and systematization and quenching prior to tempering (QT), were investigated, and their effect on the microstructure, hardness, fracture toughness (Kapp), and tempering resistance of the L-DED H13 is reported. For this purpose, the optimal austenitization schedule was identified, and tempering curves were produced. At a similar hardness level (500 HV1), QT parts showed higher Kapp (89 MPa√m) than ABT (70 MPa√m) levels. However, the fracture toughness values obtained for both parts were comparable to those of wrought H13. The slightly larger Kapp in the QT counterpart was discussed considering the microstructural homogenization and recrystallization taking place during high-temperature austenitization. The tempering resistance of the ABT material at 600 °C was slightly improved compared with that of the QT material, but for longer holding times (up to 40 h) and higher temperatures (650 °C), ABT showed superior resistance to thermal softening due to a finer martensite substructure (i.e., block size), a finer secondary carbide size, and a larger volume fraction of secondary V(C,N) carbides.
Effect of Friction Stir Welding and Tempering on the Microstructure and Strength of a Tempformed Low-Alloy Steel
Authors: Dolzhenko Anastasiia S. , Lugovskaya Anna S. , Malopheyev Sergey , Dudko Valeriy , Tikhonova Marina , Kaibyshev Rustam , Belyakov Andrey
Published: January 2024

The microstructure developed in a low-alloy steel during friction stir welding and post-weld tempering was studied. The quenched steel samples were subjected to tempering at 650 °C for 1 h, followed by warm rolling to a total strain of 1.5 at the same temperature. The processed steel samples were characterized by an ultrafine-grained microstructure of the lamellar type with a transverse grain size of 360 nm and exhibited an yield strength of about 1200 MPa and a total elongation of 13%. Then, the steel plates were joined by friction stir welding. The yield strength of the weld joint was about 1170 MPa, although the total elongation decreased to 1.5%. The martensite microstructure, with a high-angle grain boundary spacing of about 800 nm, was developed in the stir zone. This martensite in the stir zone originated from the ultrafine-grained prior austenite, resulting in an almost two-fold increase in hardness as compared to the base material. Tempering of the welded sample at 650 °C for 1 h resulted in a decrease in the hardness of the weld joint to the level of the base material. Nevertheless, the fracture of the welded and tempered sample occurred in the base material. The yield strength of the welded sample after tempering was 930 MPa, with a total elongation of 13%.
Thyroid Hormone Metabolites Quantified in Pup and Adult Rat Cerebellum, Cortex and Whole-Brain Samples Using an Automated Online SPE-LC-MS/MS Method
Authors: Hindrichs Christiane , Walk Tilmann , Landsiedel Robert , Kamp Hennicke , Schneider Steffen , Melching-Kollmuss Stephanie , Funk-Weyer Dorothee
Published: January 2024

Changes in thyroid hormone (TH) levels in rat brain at early developmental stages are correlated with adverse effects on offspring development. To characterize the ability of substances to interfere with the TH concentrations in, e.g., rat brain, it is essential to know the mean TH concentrations in this tissue under control conditions. In this publication, an online solid-phase extraction (SPE) liquid chromatography (LC) tandem mass spectrometry (MS/MS) method was validated and used to measure TH metabolites (T4, T3, rT3, T2 and T1) in the brains of untreated rats. Data on TH concentrations in the whole brain and separate data from the cerebellum and the cortex are shown. The corresponding samples were gathered from young rats at postnatal days (PND) 4 and 21/22 and from adult rats. The results show inter alia the high accuracy and precision of the method, and LOQs of 0.02 ng/mL were determined for T1, T2 and rT3 and of 0.15 ng/mL for T3 and T4. Technical variability is low, as shown by the relative standard deviations of 7.5–20%. For our rat model, we found that T4, T3 and T2 concentrations rise from PND4 to PND21, whereas the rT3 concentration decreases; as well as there is no statistical difference between TH concentrations in the male and female rat brain. This method is suitable to analyze TH metabolites in the brain and build up a database of historical TH concentrations in control rats. Together, this yields a robust diagnostic tool to detect potentially adverse disturbances of TH homeostasis in the most vulnerable anatomic structure.
Micromotors of MnO2 for the Recovery of Microplastics
Authors: Cervantes Oscar , Valtierra-Montiel Claudia , Sampedro-Plata Laura , Casillas Norberto , Menendez Nieves , Herrasti Pilar
Published: January 2024

Plastics, primarily microplastics, are among the greatest pollutants in aquatic environments. Their removal and/or degradation in these environments are crucial to ensure an optimal future of these ecosystems. In this work, MnO2 particles were synthesized and characterized for the removal of polystyrene microplastics as a model. MnO2 catalyzes the peroxide reaction, resulting in the formation of oxygen bubbles that propel the pollutants to the surface, achieving removal efficiencies of up to 80%. To achieve this, hydrothermal synthesis was employed using various methods. Parameters such as MnO2, pH, microplastics, and H2O2 concentrations were varied to determine the optimal conditions for microplastics recovering. The ideal conditions for a low microplastic concentrations (10 mg L−1) are 0.2 g L−1 MnO2, 1.6% of H2O2 and 0.01 triton as a surfactant. In these conditions, the micromotors can recover approximately 80% of 300 nm sized polystyrene microplastic within 40 min.
A Novel Compact Broadband Quasi-Twisted Branch Line Coupler Based on a Double-Layered Microstrip Line
Authors: Ahmed Fayyadh H. , Saad Rola , Khamas Salam K.
Published: January 2024

A novel quasi-twisted miniaturized wideband branch line coupler (BLC) is proposed. The design is based on bisecting the conventional microstrip line BLC transversely and folding bisected sections on double-layered substrates with a common ground plane in between. The input and output terminals, each with a length of λg/4, and the pair of quarter-wavelength horizontal parallel arms are converted into a Z-shaped meandered microstrip line in the designed structure. Conversely, the pair of quarter-wavelength vertical arms are halved into two lines and transformed into a periodically loaded slow-wave structure. The bisected parts of the BLC are placed on the opposite side of the doubled-layer substrate and connected through four vias passing through the common ground plane. This technique enabled a compact BLC size of 6.4 × 18 mm2, which corresponds to a surface area miniaturization by ~50% as compared to the classical BLC size of 10 × 23 mm2 at 6 GHz. Moreover, the attained relative bandwidth is 73.9% (4.6–10 GHz) for S11, S33, S21, and the phase difference between outputs (∠S21 − ∠S41). However, if a coupling parameter (S41) of up to −7.5 dB is considered, then the relative bandwidth reduces to 53.9% (4.6–10 GHz) for port 1 as the input. Similarly, for port 3 as the input, the obtained bandwidth is 75.8% (4.5–10 GHz) for S33, S11, S43, and the phase difference between outputs (∠S43 − ∠S23). Likewise, this bandwidth reduces to 56% (4.5–8 GHz) when a coupling parameter (S23) of up to −7.5 dB is considered. In contrast, the relative bandwidth for the ordinary BLC is 41% at the same resonant frequency. The circuit is constructed on a double-layered low-cost FR4 substrate with a relative permittivity of 4.3 and a loss tangent of 0.025. An isolation of −13 dB was realized in both S13 and S31 demonstrating an excellent performance. The transmission coefficients between input/output ports S21, S41, S23, and S43 are between −3.1 dB to −3.5 dB at a frequency of 6 GHz. Finally, the proposed BLC provides phase differences between output ports of 90.5° and 94.8° at a frequency of 6 GHz when the input ports 1 and 3 are excited, respectively. The presented design offers the potential of being utilized as a unit cell for building a Butler matrix (BM) for sub-6 GHz 5G beamforming networks.
A Study on the Surface Quality and Damage Properties of Single-Crystal Silicon Using Different Post-Treatment Processes
Authors: Li Wei , Zha Fangyuan , Fu Bo , Li Yanglong , Duan Jiazhu , Zhou Ziyou
Published: January 2024

Detecting subsurface defects in optical components has always been challenging. This study utilizes laser scattering and photothermal weak absorption techniques to detect surface and subsurface nano-damage precursors of single-crystal silicon components. Based on laser scattering and photothermal weak absorption techniques, we successfully establish the relationship between damage precursors and laser damage resistance. The photothermal absorption level is used as an important parameter to measure the damage resistance threshold of optical elements. Single-crystal silicon elements are processed and post-processed optimally. This research employs dry etching and wet etching techniques to effectively eliminate damage precursors from optical components. Additionally, detection techniques are utilized to comprehensively characterize these components, resulting in the successful identification of optimal damage precursor removal methods for various polishing types of single-crystal silicon components. Consequently, this method efficiently enhances the damage thresholds of optical components.
Exploring Bacterial Cellulose and a Biosurfactant as Eco-Friendly Strategies for Addressing Pharmaceutical Contaminants
Authors: Castanho Nathália Roberta Cardoso Mendes , de Marco Nathane , Caetano Érika Leão Ajala , Alves Patrícia Lius Melo , Pickler Thaisa Borim , Ibanez Natasha Lien de Almeida , Jozala Angela Faustino , Grotto Denise
Published: January 2024

Aquatic environments face contamination by pharmaceuticals, prompting concerns due to their toxicity even at low concentrations. To combat this, we developed an ecologically sustainable biosurfactant derived from a microorganism and integrated it into bacterial cellulose (BC). This study aimed to evaluate BC’s efficacy, with and without the biosurfactant, as a sorbent for paracetamol and 17α-ethinylestradiol (EE2) in water. We cultivated BC membranes using Gluconacetobacter xylinus ATCC 53582 and synthesized the biosurfactant through pre-inoculation of Bacillus subtilis in a synthetic medium. Subsequently, BC membranes were immersed in the biosurfactant solution for incorporation. Experiments were conducted using contaminated water, analyzing paracetamol concentrations via spectrophotometry and EE2 levels through high-performance liquid chromatography. Results indicated BC’s superior adsorption for EE2 over paracetamol. Incorporating the biosurfactant reduced hormone adsorption but enhanced paracetamol sorption. Notably, original and freeze-dried BC exhibited better adsorption efficacy than biosurfactant-infused BC. In conclusion, BC showed promise in mitigating EE2 contamination, suggesting its potential for environmental remediation. Future research could focus on optimizing biosurfactant concentrations to enhance sorption capabilities without compromising BC’s inherent effectiveness.
Environmental Analytical Chemistry
Authors: Tsakovski Stefan , Venelinov Tony
Published: January 2024

Environmental analytical chemistry has evolved into a well-established interdisciplinary field (analytical chemistry, pollution chemistry, chemical engineering, etc [...]
Influence of Combinations of Lipophilic and Phosphate Backbone Modifications on Cellular Uptake of Modified Oligonucleotides
Authors: Zharkov Timofey D. , Markov Oleg V. , Zhukov Sergey A. , Khodyreva Svetlana N. , Kupryushkin Maxim S.
Published: January 2024

Numerous types of oligonucleotide modifications have been developed since automated synthesis of DNA/RNA became a common instrument in the creation of synthetic oligonucleotides. Despite the growing number of types of oligonucleotide modifications under development, only a few of them and, moreover, their combinations have been studied widely enough in terms of their influence on the properties of corresponding NA constructions. In the present study, a number of oligonucleotides with combinations of 3′-end lipophilic (a single cholesteryl or a pair of dodecyl residues) and phosphate backbone modifications were synthesized. The influence of the combination of used lipophilic groups with phosphate modifications of various natures and different positions on the efficiency of cell penetration was evaluated. The obtained results indicate that even a couple of phosphate modifications are able to affect a set of oligonucleotide properties in a complex manner and can remarkably change cellular uptake. These data clearly show that the strategy of using different patterns of modification combinations has great potential for the rational design of oligonucleotide structures with desired predefined properties.
Identification of GM1-Ganglioside Secondary Accumulation in Fibroblasts from Neuropathic Gaucher Patients and Effect of a Trivalent Trihydroxypiperidine Iminosugar Compound on Its Storage Reduction
Authors: Ceni Costanza , Clemente Francesca , Mangiavacchi Francesca , Matassini Camilla , Tonin Rodolfo , Caciotti Anna , Feo Federica , Coviello Domenico , Morrone Amelia , Cardona Francesca , Calamai Martino
Published: January 2024

Gaucher disease (GD) is a rare genetic metabolic disorder characterized by a dysfunction of the lysosomal glycoside hydrolase glucocerebrosidase (GCase) due to mutations in the gene GBA1, leading to the cellular accumulation of glucosylceramide (GlcCer). While most of the current research focuses on the primary accumulated material, lesser attention has been paid to secondary storage materials and their reciprocal intertwining. By using a novel approach based on flow cytometry and fluorescent labelling, we monitored changes in storage materials directly in fibroblasts derived from GD patients carrying N370S/RecNcil and homozygous L444P or R131C mutations with respect to wild type. In L444P and R131C fibroblasts, we detected not only the primary accumulation of GlcCer accumulation but also a considerable secondary increase in GM1 storage, comparable with the one observed in infantile patients affected by GM1 gangliosidosis. In addition, the ability of a trivalent trihydroxypiperidine iminosugar compound (CV82), which previously showed good pharmacological chaperone activity on GCase enzyme, to reduce the levels of storage materials in L444P and R131C fibroblasts was tested. Interestingly, treatment with different concentrations of CV82 led to a significant reduction in GM1 accumulation only in L444P fibroblasts, without significantly affecting GlcCer levels. The compound CV82 was selective against the GCase enzyme with respect to the β-Galactosidase enzyme, which was responsible for the catabolism of GM1 ganglioside. The reduction in GM1-ganglioside level cannot be therefore ascribed to a direct action of CV82 on β-Galactosidase enzyme, suggesting that GM1 decrease is rather related to other unknown mechanisms that follow the direct action of CV82 on GCase. In conclusion, this work indicates that the tracking of secondary storages can represent a key step for a better understanding of the pathways involved in the severity of GD, also underlying the importance of developing drugs able to reduce both primary and secondary storage-material accumulations in GD.
Anti-PD-L1-Based Bispecific Antibodies Targeting Co-Inhibitory and Co-Stimulatory Molecules for Cancer Immunotherapy
Authors: Geng Qiaohong , Jiao Peifu
Published: January 2024

Targeting PD-L1 via monospecific antibodies has shown durable clinical benefits and long-term remissions where patients exhibit no clinical cancer signs for many years after treatment. However, the durable clinical benefits and long-term remissions by anti-PD-L1 monotherapy have been limited to a small fraction of patients with certain cancer types. Targeting PD-L1 via bispecific antibodies (referred to as anti-PD-L1-based bsAbs) which can simultaneously bind to both co-inhibitory and co-stimulatory molecules may increase the durable antitumor responses in patients who would not benefit from PD-L1 monotherapy. A growing number of anti-PD-L1-based bsAbs have been developed to fight against this deadly disease. This review summarizes recent advances of anti-PD-L1-based bsAbs for cancer immunotherapy in patents and literatures, and discusses their anti-tumor efficacies in vitro and in vivo. Over 50 anti-PD-L1-based bsAbs targeting both co-inhibitory and co-stimulatory molecules have been investigated in biological testing or in clinical trials since 2017. At least eleven proteins, such as CTLA-4, LAG-3, PD-1, PD-L2, TIM-3, TIGIT, CD28, CD27, OX40, CD137, and ICOS, are involved in these investigations. Twenty-two anti-PD-L1-based bsAbs are being evaluated to treat various advanced cancers in clinical trials, wherein the indications include NSCLC, SNSCLC, SCLC, PDA, MBNHL, SCCHN, UC, EC, TNBC, CC, and some other malignancies. The released data from clinical trials indicated that most of the anti-PD-L1-based bsAbs were well-tolerated and showed promising antitumor efficacy in patients with advanced solid tumors. However, since the approved and investigational bsAbs have shown much more significant adverse reactions compared to PD-L1 monospecific antibodies, anti-PD-L1-based bsAbs may be further optimized via molecular structure modification to avoid or reduce these adverse reactions.
Polymorphism and Red Photoluminescence Emission from 5s2 Electron Pairs of Sb(III) in a New One-Dimensional Organic–Inorganic Hybrid Based on Methylhydrazine: MHy2SbI5
Authors: Rowińska Magdalena , Stefańska Dagmara , Bednarchuk Tamara J. , Zaręba Jan K. , Jakubas Ryszard , Gągor Anna
Published: January 2024

We explore the crystal structure and luminescent properties of a new 1D organic–inorganic hybrid, MHy2SbI5, based on methylhydrazine. The compound reveals the red photoluminescence (PL) originating from the 5s2 electron pairs of Sb(III) as well as complex structural behavior. MHy2SbI5 crystalizes in two polymorphic forms (I and II) with distinct thermal properties and structural characteristics. Polymorph I adopts the acentric P212121 chiral space group confirmed by SHG, and, despite a thermally activated disorder of MHy, does not show any phase transitions, while polymorph II undergoes reversible low-temperature phase transition and high-temperature reconstructive transformation to polymorph I. The crystal structures of both forms consist of 1D perovskite zig-zag chains of corner-sharing SbI6 octahedra. The intriguing phase transition behavior of II is associated with the unstable arrangement of the [SbI5]2−∞ chains in the structure. The energy band gap (Eg) values, estimated based on the UV-Vis absorption spectra, indicate that both polymorphs have band gaps, with Eg values of 2.01 eV for polymorph I and 2.12 eV for polymorph II.
Effects of Chalcogen Atoms on Excited-State Double-Proton Transfer Behavior for 3,6-bis(4,5-Dihydroxyoxazo-2-yl)benzene-1,2-diol Derivatives: A Computational Investigation
Authors: Yang Dapeng , Liu Chang , Zhang Meiyi , Zhao Jinfeng
Published: January 2024

The impact of the chalcogen atomic electronegativity (O, S, and Se atoms) of new organic molecules on excited-state dynamical reactions is self-evident. Inspired by this kind of distinguished photochemical characteristic, in this work, we performed a computational investigation of chalcogen-substituted 3,6-bis(4,5-dihydroxyoxazo-2-yl)benzene-1,2-diol (BDYBD) derivatives (i.e., BDYBD-O, BDYBD-S, and BDYBD-Se). In this paper, we pay close attention to characteristic BDYBD derivatives that contain intramolecular double hydrogen bonds (O1–H2···N3 and O4–H5···N6). The main goal of this study was to explore how changes in atomic electronegativity affect the way hydrogen bonds interact and how excited molecules affect transfer protons. We go into further detail in the main text of the paper. By fixing our attention to geometrical variations and infrared (IR) vibrational spectra between the S0 and S1 states, exploring hydrogen bonding behaviors using the core-valence bifurcation (CVB) index, and simulating hydrogen bonding energy (EHB) via the atom in molecule (AIM) method, we clarified the photo-induced strengthened dual hydrogen bonding interactions that facilitate the excited-state dual-proton transfer (ESDPT) behavior of BDYBD derivatives. The reorganization of charge stemming from photoexcitation further verifies the tendencies of ESDPT reactions. We relied on constructing potential energy surfaces (PESs) by adopting a restrictive optimization approach, and herein, we finally clarify the gradual ESDPT mechanism of BDYBD derivatives. Particularly, we confirm that the variation in chalcogen atomic electronegativity has a regulatory effect on the ESDPT behavior of BDYBD derivatives; that is, the lower the atomic electronegativity, the more favorable it is for the gradual ESDPT reaction.
A Facile Ugi/Ullmann Cascade Reaction to Access Fused Indazolo-Quinoxaline Derivatives with Potent Anticancer Activity
Authors: Li Yong , He Liujun , Qin Hongxia , Liu Yao , Yang Binxin , Xu Zhigang , Yang Donglin
Published: January 2024

A facile methodology for the construction of a complex heterocycle indazolo-fused quinoxalinone has been developed via an Ugi four-component reaction (U-4CR) followed by an intramolecular Ullmann reaction. The expeditious process features an operationally simple approach, time efficiency, and a broad substrate scope. Biological activity was evaluated and demonstrated that compound 6e inhibits human colon cancer cell HCT116 proliferation with an IC50 of 2.1 μM, suggesting potential applications for developing a drug lead in medicinal chemistry.