bluuebunny/crossref_metadata_2025 huggingface dataset:
OFP1 Interaction with ATH1 Regulates Stem Growth, Flowering Time and Flower Basal Boundary Formation in Arabidopsis
Authors: Zhang Liguo , Sun Lili , Zhang Xiaofei , Zhang Shuquan , Xie Dongwei , Liang Chunbo , Huang Wengong , Fan Lijuan , Fang Yuyan , Chang Ying
Published: August 2018

Ovate Family Protein1 (OFP1) is a regulator, and it is suspected to be involved in plant growth and development. Meanwhile, Arabidopsis Thaliana Homeobox (ATH1), a BEL1-like homeodomain (HD) transcription factor, is known to be involved in regulating stem growth, flowering time and flower basal boundary development in Arabidopsis. Previous large-scale yeast two-hybrid studies suggest that ATH1 possibly interact with OFP1, but this interaction is yet unverified. In our study, the interaction of OFP1 with ATH1 was verified using a directional yeast two-hybrid system and bimolecular fluorescence complementation (BiFC). Our results also demonstrated that the OFP1-ATH1 interaction is mainly controlled by the HD domain of ATH1. Meanwhile, we found that ATH1 plays the role of transcriptional repressor to regulate plant development and that OFP1 can enhance ATH1 repression function. Regardless of the mechanism, a putative functional role of ATH1-OFP1 may be to regulate the expression of the both the GA20ox1 gene, which is involved in gibberellin (GA) biosynthesis and control of stem elongation, and the Flowering Locus C (FLC) gene, which inhibits transition to flowering. Ultimately, the regulatory functional mechanism of OFP1-ATH1 may be complicated and diverse according to our results, and this work lays groundwork for further understanding of a unique and important protein–protein interaction that influences flowering time, stem development, and flower basal boundary development in plants.
Combining Social Vulnerability and Physical Vulnerability to Analyse Landslide Risk at the Municipal Scale
Authors: Guillard-Gonçalves Clémence , Zêzere José Luís
Published: August 2018

In this work residents’ social vulnerability and buildings’ physical vulnerability of the Loures municipality (Portugal) were combined to locate the areas where the vulnerability is the highest, and to analyse the landslide risk. The social vulnerability of Loures was assessed using the Geographic Basis for Information Reference (BGRI) terrain units by combining sensitivity and lack of resilience based on the population and housing Census 2011 data. The physical vulnerability was assessed in a previous study based on an inquiry of a pool of European landslide experts and a sub-pool of landslide experts who know the study area. A matrix approach was used to cross the classes of the social and physical vulnerabilities. Finally, the landslide risk was analysed for each terrain unit considering the combined vulnerability, the buildings’ economic value and the landslide susceptibility for a specific landslide magnitude (3-metre-deep rotational slide). Results show that 0.9% of the population reside in the area of the municipality where 75% of the future landslide should occur, and 0.8% of the buildings of the municipality—which represent a value of EUR 146,170,000—are also located in this dangerous area. This approach is reproducible: the risk analysis can be applied for another magnitude scenario in Loures, and the combined vulnerability can be assessed in any Portuguese municipality thanks to the availability of the data.
Soil Pollution and Remediation
Authors: Paz-Ferreiro Jorge , Gascó Gabriel , Méndez Ana , Reichman Suzie
Published: August 2018

n/a
Micronuclei in Exfoliated Buccal Cells of Children Living in a Cluster Area of Salento (Southern Italy) with a High Incidence of Lung Cancer: The IMP.AIR Study
Authors: Idolo Adele , Grassi Tiziana , Bagordo Francesco , Panico Alessandra , De Giorgi Mattia , Serio Francesca , Guido Marcello , Piscitelli Prisco , De Filippis Giovanni , Raho Annamaria , De Donno Antonella
Published: August 2018

During the years 2014–2016 the University of Salento performed the “Impact of Air Quality on Health of Residents in the Municipalities of Cutrofiano, Galatina, Sogliano Cavour, Soleto and Sternatia” (IMP.AIR) study, an epidemiological-molecular research project aiming to evaluate early DNA damage in children living in an area of Salento with high incidence of lung cancer among the male population. One hundred and twenty-two children aged 6–8 years attending primary school were enrolled and the frequency of micronucleated cells (MNC) in oral mucosa was evaluated. In addition, a questionnaire was administered to parents to obtain information about personal data, anthropometric characteristics and lifestyles (physical activity, food habits, family context) of the children and perform a multivariate analysis to detect any factors associated with MNC occurrence. Data on airborne pollutants detected in the study area were acquired by the Regional Agency for the Environmental Protection. The presence of MNC was highlighted in about 42% of children with a mean MNC frequency of 0.49‰. The frequency of MNC was associated to obesity, consumption of red or processed meat and having a mother who smokes. Moreover, the prevalence of biomarkers was higher than in another area of Salento not included in the cluster area.
Meta-Prediction of MTHFR Gene Polymorphism and Air Pollution on the Risks of Congenital Heart Defects Worldwide: A Transgenerational Analysis
Authors: Yang Hsiao-Ling , Yang Ya-Ling , Yu Chong , Shiao S.
Published: August 2018

Congenital heart disease (CHD) is the leading cause of death in children, and is affected by genetic and environmental factors. To investigate the association of air pollution with methylene-tetrahydrofolate reductase (MTHFR) polymorphisms and the risk of CHD, we included 58 study groups of children and parents, with 12,347 cases and 18,106 controls worldwide. Both MTHFR C677T (rs 1801133) and A1298C (rs 1801131) gene polymorphisms were risks for CHD in children with transgenerational effects from their parents. Countries with greater risks of CHD with a pooled risk ratio (RR) > 2 from MTHFR 677 polymorphisms included Germany, Portugal, China, and Egypt for children; and Brazil, Puerto Rico, Mexico, China, and Egypt for mothers. Whereas, countries with greater risk of CHD with RR > 2 from MTHFR 1298 polymorphisms included Taiwan, Turkey, and Egypt for children; and Brazil, China, and Egypt for mothers. Additionally, meta-prediction analysis revealed that the percentages of MTHFR 677TT and TT plus CT polymorphisms together were increased in countries with higher levels of air pollution, with a trend of increased CHD risks with higher levels of air pollution for children (p = 0.07). Our findings may have significant implications for inflammatory pathways in association with MTHFR polymorphisms and future intervention studies to correct for folate-related enzyme deficits resulted from MTHFR polymorphisms to prevent CHDs for future generations.
Diarrhoeal Disease in Relation to Possible Household Risk Factors in South African Villages
Authors: Kapwata Thandi , Mathee Angela , Le Roux Wouter Jacobus , Wright Caradee Yael
Published: August 2018

Diarrhoeal disease is a significant contributor to child morbidity and mortality, particularly in the developing world. Poor sanitation, a lack of personal hygiene and inadequate water supplies are known risk factors for diarrhoeal disease. Since risk factors may vary by population or setting, we evaluated the prevalence of diarrhoeal disease at the household level using a questionnaire to better understand household-level risk factors for diarrhoea in selected rural areas in South Africa. In a sub-sample of dwellings, we measured the microbial quality of drinking water. One in five households had at least one case of diarrhoea during the previous summer. The most widespread source of drinking water was a stand-pipe (inside yard) (45%) followed by an indoor tap inside the dwelling (29%). Storage of water was common (97%) with around half of households storing water in plastic containers with an opening large enough to fit a hand through. After adjusting for confounders, the occurrence of diarrhoea was statistically significantly associated with sourcing water from an indoor tap (Adjusted Odds Ratio (AOR): 2.73, 95% CI: 2.73, 1.14–6.56) and storing cooked/perishable food in non-refrigerated conditions (AOR: 2.17, 95% CI: 2.17, 1.44–3.26). The highest total coliform counts were found in water samples from kitchen containers followed by stand-pipes. Escherichia coli were most often detected in samples from stand-pipes and kitchen containers. One in four households were at risk of exposure to contaminated drinking water, increasing the susceptibility of the study participants to episodes of diarrhoea. It is imperative that water quality meets guideline values and routine monitoring of quality of drinking water is done to minimise diarrhoea risk in relevant rural communities. The security of water supply in rural areas should be addressed as a matter of public health urgency to avoid the need for water storage.
Do Water, Sanitation and Hygiene Conditions in Primary Schools Consistently Support Schoolgirls’ Menstrual Needs? A Longitudinal Study in Rural Western Kenya
Authors: Alexander Kelly T. , Zulaika Garazi , Nyothach Elizabeth , Oduor Clifford , Mason Linda , Obor David , Eleveld Alie , Laserson Kayla F. , Phillips-Howard Penelope A.
Published: August 2018

Many females lack access to water, privacy and basic sanitation—felt acutely when menstruating. Water, sanitation and hygiene (WASH) conditions in schools, such as access to latrines, water, and soap, are essential for the comfort, equity, and dignity of menstruating girls. Our study was nested within a cluster randomized controlled pilot feasibility study where nurses provided menstrual items to schoolgirls. We observed the WASH conditions of 30 schools from June 2012–October 2013 to see if there were any changes in conditions, to compare differences between study arms and to examine agreement between observed and teacher-reported conditions. Data came from study staff observed, and school teacher reported, WASH conditions. We developed scores for the condition of school facilities to report any changes in conditions and compare outcomes across study arms. Results demonstrated that soap availability for students increased significantly between baseline and follow-up while there was a significant decrease in the number of “acceptable” latrines. During the study follow-up period, individual WASH indicators supporting menstruating girls, such as locks on latrine doors or water availability in latrines did not significantly improve. Advances in WASH conditions for all students, and menstrual hygiene facilities for schoolgirls, needs further support, a defined budget, and regular monitoring of WASH facilities to maintain standards.
The Overlooked Burden of Food Insecurity among Asian Americans: Results from the California Health Interview Survey
Authors: Becerra Monideepa B. , Mshigeni Salome Kapella , Becerra Benjamin J.
Published: August 2018

Objective: Food insecurity remains a major public health issue in the United States, though lack of research among Asian Americans continue to underreport the issue. The purpose of this study was to evaluate the prevalence and burden of food insecurity among disaggregated Asian American populations. Methods: The California Health Interview Survey, the largest state health survey, was used to assess the prevalence of food insecurity among Asian American subgroups with primary exposure variable of interest being acculturation. Survey-weighted descriptive, bivariate, and multivariable robust Poisson regression analyses, were conducted and alpha less than 0.05 was used to denote significance. Results: The highest prevalence of food insecurity was found among Vietnamese (16.42%) and the lowest prevalence was among Japanese (2.28%). A significant relationship was noted between prevalence of food insecurity and low acculturation for Chinese, Korean, and Vietnamese subgroups. Language spoken at home was significant associated with food insecurity. For example, among Chinese, being food insecure was associated with being bilingual (prevalence ratio [PR] = 2.51) or speaking a non-English language at home (PR = 7.24), while among South Asians, it was associated with speaking a non-English language at home was also related to higher prevalence (PR = 3.62), as compared to English speakers only. Likewise, being foreign-born also related to being food insecure among Chinese (PR = 2.31), Filipino (PR = 1.75), South Asian (PR = 3.35), Japanese (PR = 2.11), and Vietnamese (PR = 3.70) subgroups, when compared to their US-born counterparts. Conclusion: There is an imperative need to address food insecurity burden among Asian Americans, especially those who have low acculturation.
Factors Associated with Heritable Pulmonary Arterial Hypertension Exert Convergent Actions on the miR-130/301-Vascular Matrix Feedback Loop
Authors: Bertero Thomas , Handen Adam L. , Chan Stephen Y.
Published: August 2018

Pulmonary arterial hypertension (PAH) is characterized by occlusion of lung arterioles, leading to marked increases in pulmonary vascular resistance. Although heritable forms of PAH are known to be driven by genetic mutations that share some commonality of function, the extent to which these effectors converge to regulate shared processes in this disease is unknown. We have causally connected extracellular matrix (ECM) remodeling and mechanotransduction to the miR-130/301 family in a feedback loop that drives vascular activation and downstream PAH. However, the molecular interconnections between factors genetically associated with PAH and this mechano-driven feedback loop remain undefined. We performed systematic manipulation of matrix stiffness, the miR-130/301 family, and factors genetically associated with PAH in primary human pulmonary arterial cells and assessed downstream and reciprocal consequences on their expression. We found that a network of factors linked to heritable PAH converges upon the matrix stiffening-miR-130/301-PPARγ-LRP8 axis in order to remodel the ECM. Furthermore, we leveraged a computational network biology approach to predict a number of additional molecular circuits functionally linking this axis to the ECM. These results demonstrate that multiple genes associated with heritable PAH converge to control the miR-130/301 circuit, triggering a self-amplifying feedback process central to pulmonary vascular stiffening and disease.
The Micro-RNA Expression Profiles of Autoimmune Arthritis Reveal Novel Biomarkers of the Disease and Therapeutic Response
Authors: Dudics Steven , Venkatesha Shivaprasad H. , Moudgil Kamal D.
Published: August 2018

Rheumatoid arthritis (RA) is a chronic autoimmune disease of the joints affecting about 0.3–1% of the population in different countries. About 50–60 percent of RA patients respond to presently used drugs. Moreover, the current biomarkers for RA have inherent limitations. Consequently, there is a need for additional, new biomarkers for monitoring disease activity and responsiveness to therapy of RA patients. We examined the micro-RNA (miRNA) profile of immune (lymphoid) cells of arthritic Lewis rats and arthritic rats treated with celastrol, a natural triterpenoid. Experimental and bioinformatics analyses revealed 8 miRNAs (miR-22, miR-27a, miR-96, miR-142, miR-223, miR-296, miR-298, and miR-451) and their target genes in functional pathways important for RA pathogenesis. Interestingly, 6 of them (miR-22, miR-27a, miR-96, miR-142, miR-223, and miR-296) were further modulated by celastrol treatment. Interestingly, serum levels of miR-142, miR-155, and miR-223 were higher in arthritic versus control rats, whereas miR-212 showed increased expression in celastrol-treated rats compared with arthritic rats or control rats. This is the first study on comprehensive miRNA expression profiling in the adjuvant-induced arthritis (AA) model and it also has revealed new miRNA targets for celastrol in arthritis. We suggest that subsets of the above miRNAs may serve as novel biomarkers of disease activity and therapeutic response in arthritis.
AAV-Syn-BDNF-EGFP Virus Construct Exerts Neuroprotective Action on the Hippocampal Neural Network during Hypoxia In Vitro
Authors: Mitroshina Elena , Mishchenko Tatiana , Usenko Alexandra , Epifanova Ekaterina , Yarkov Roman , Gavrish Maria , Babaev Alexey , Vedunova Maria
Published: August 2018

Brain-derived neurotrophic factor (BDNF) is one of the key signaling molecules that supports the viability of neural cells in various brain pathologies, and can be considered a potential therapeutic agent. However, several methodological difficulties, such as overcoming the blood–brain barrier and the short half-life period, challenge the potential use of BDNF in clinical practice. Gene therapy could overcome these limitations. Investigating the influence of viral vectors on the neural network level is of particular interest because viral overexpression affects different aspects of cell metabolism and interactions between neurons. The present work aimed to investigate the influence of the adeno-associated virus (AAV)-Syn-BDNF-EGFP virus construct on neural network activity parameters in an acute hypobaric hypoxia model in vitro. Materials and methods. An adeno-associated virus vector carrying the BDNF gene was constructed using the following plasmids: AAV-Syn-EGFP, pDP5, DJvector, and pHelper. The developed virus vector was then tested on primary hippocampal cultures obtained from C57BL/6 mouse embryos (E18). Acute hypobaric hypoxia was induced on day 21 in vitro. Spontaneous bioelectrical and calcium activity of neural networks in primary cultures and viability tests were analysed during normoxia and during the posthypoxic period. Results. BDNF overexpression by AAV-Syn-BDNF-EGFP does not affect cell viability or the main parameters of spontaneous bioelectrical activity in normoxia. Application of the developed virus construct partially eliminates the negative hypoxic consequences by preserving cell viability and maintaining spontaneous bioelectrical activity in the cultures. Moreover, the internal functional structure, including the activation pattern of network bursts, the number of hubs, and the number of connections within network elements, is also partially preserved. BDNF overexpression prevents a decrease in the number of cells exhibiting calcium activity and maintains the frequency of calcium oscillations. Conclusion. This study revealed the pronounced antihypoxic and neuroprotective effects of AAV-Syn-BDNF-EGFP virus transduction in an acute normobaric hypoxia model.
Pan-Cancer Analysis Reveals Differential Susceptibility of Bidirectional Gene Promoters to DNA Methylation, Somatic Mutations, and Copy Number Alterations
Authors: Thompson Jeffrey A. , Christensen Brock C. , Marsit Carmen J.
Published: August 2018

Bidirectional gene promoters affect the transcription of two genes, leading to the hypothesis that they should exhibit protection against genetic or epigenetic changes in cancer. Therefore, they provide an excellent opportunity to learn about promoter susceptibility to somatic alteration in tumors. We tested this hypothesis using data from genome-scale DNA methylation (14 cancer types), simple somatic mutation (10 cancer types), and copy number variation profiling (14 cancer types). For DNA methylation, the difference in rank differential methylation between tumor and tumor-adjacent normal matched samples based on promoter type was tested by the Wilcoxon rank sum test. Logistic regression was used to compare differences in simple somatic mutations. For copy number alteration, a mixed effects logistic regression model was used. The change in methylation between non-diseased tissues and their tumor counterparts was significantly greater in single compared to bidirectional promoters across all 14 cancer types examined. Similarly, the extent of copy number alteration was greater in single gene compared to bidirectional promoters for all 14 cancer types. Furthermore, among 10 cancer types with available simple somatic mutation data, bidirectional promoters were slightly more susceptible. These results suggest that selective pressures related with specific functional impacts during carcinogenesis drive the susceptibility of promoter regions to somatic alteration.
The Winding Roadmap of Biomarkers Toward Clinic: Lessons from Predictors of Resistance to Anti-EGFRs in Metastatic Colorectal Cancer
Authors: Antoniotti Carlotta , Ongaro Elena , Falcone Alfredo , Cremolini Chiara
Published: August 2018

In the evolving molecular landscape of metastatic colorectal cancer, optimizing available tools to select patients to receive anti-epidermal growth factor receptor (anti-EGFR) monoclonal antibodies is a modern challenge of colorectal oncologists. Several molecular biomarkers have been investigated in recent years as potential predictors of resistance to anti-EGFR agents in preclinical and clinical retrospective series. Nevertheless, none of them have been implemented in clinical practice due to the lack of a formal prospective demonstration. Here, we propose a literature review of molecular alterations associated with resistance to anti-EGFRs, underlining the reasons why their roadmap from laboratories to clinics was prematurely halted.
Contribution of STAT3 to Inflammatory and Fibrotic Diseases and Prospects for its Targeting for Treatment
Authors: Kasembeli Moses M. , Bharadwaj Uddalak , Robinson Prema , Tweardy David J.
Published: August 2018

Signal transducer and activator of transcription (STAT) 3 plays a central role in the host response to injury. It is activated rapidly within cells by many cytokines, most notably those in the IL-6 family, leading to pro-proliferative and pro-survival programs that assist the host in regaining homeostasis. With persistent activation, however, chronic inflammation and fibrosis ensue, leading to a number of debilitating diseases. This review summarizes advances in our understanding of the role of STAT3 and its targeting in diseases marked by chronic inflammation and/or fibrosis with a focus on those with the largest unmet medical need.
A Short Half-Life αIIbβ3 Antagonist ANTP266 Reduces Thrombus Formation
Authors: Liu Tong-Dan , Ren Shen-Hong , Ding Xue , Xie Zhou-Ling , Kong Yi
Published: August 2018

Integrin αIIbβ3 plays a pivotal role in platelet aggregation. Three αIIbβ3 antagonists have been approved by the Food and Drug Administration (FDA) for the treatment of cardiovascular diseases. Unfortunately, all of these three drugs can cause the side effect of severe bleeding. Therefore, developing a new αIIbβ3 antagonist with low bleeding was needed. In the present study, we screened compounds by using a fibrinogen/integrin αIIbβ3 enzyme-linked immunosorbent assay (ELISA), and a novel αIIbβ3 antagonist ANTP266 was attained. The antithrombotic effects of ANTP266 were estimated by using two animal models, the bleeding risk was estimated by using a mice tail cutting assay, and the plasma half-life time was tested by LC-MS/MS. The results showed that ANTP266 potently decreased thrombosis formation, while not prolonging bleeding time at its effective dosage. The bleeding of ANTP266 reduced rapidly as time went on from 5 to 60 min, but tirofiban produced high bleeding continuously. The plasma half-life of ANTP266 in rats was 10.8 min. Taken together, ANTP266 is an effective antithrombotic agent with a low bleeding risk. The shorter bleeding time benefits from its short plasma half-life. ANTP266 could be a candidate for developing the αIIbβ3 antagonist of rapid elimination for a patient undergoing percutaneous coronary intervention.
CTLA-4 Mediates Inhibitory Function of Mesenchymal Stem/Stromal Cells
Authors: Gaber Timo , Schönbeck Kerstin , Hoff Holger , Tran Cam Loan , Strehl Cindy , Lang Annemarie , Ohrndorf Sarah , Pfeiffenberger Moritz , Röhner Eric , Matziolis Georg , Burmester Gerd-R. , Buttgereit Frank , Hoff Paula
Published: August 2018

Mesenchymal stem/stromal cells (MSCs) are stem cells of the connective tissue, possess a plastic phenotype, and are able to differentiate into various tissues. Besides their role in tissue regeneration, MSCs perform additional functions as a modulator or inhibitor of immune responses. Due to their pleiotropic function, MSCs have also gained therapeutic importance for the treatment of autoimmune diseases and for improving fracture healing and cartilage regeneration. However, the therapeutic/immunomodulatory mode of action of MSCs is largely unknown. Here, we describe that MSCs express the inhibitory receptor CTLA-4 (cytotoxic T lymphocyte antigen 4). We show that depending on the environmental conditions, MSCs express different isoforms of CTLA-4 with the secreted isoform (sCTLA-4) being the most abundant under hypoxic conditions. Furthermore, we demonstrate that the immunosuppressive function of MSCs is mediated mainly by the secretion of CTLA-4. These findings open new ways for treatment when tissue regeneration/fracture healing is difficult.
The Aphid-Transmitted Turnip yellows virus Differentially Affects Volatiles Emission and Subsequent Vector Behavior in Two Brassicaceae Plants
Authors: Claudel Patricia , Chesnais Quentin , Fouché Quentin , Krieger Célia , Halter David , Bogaert Florent , Meyer Sophie , Boissinot Sylvaine , Hugueney Philippe , Ziegler-Graff Véronique , Ameline Arnaud , Brault Véronique
Published: August 2018

Aphids are important pests which cause direct damage by feeding or indirect prejudice by transmitting plant viruses. Viruses are known to induce modifications of plant cues in ways that can alter vector behavior and virus transmission. In this work, we addressed whether the modifications induced by the aphid-transmitted Turnip yellows virus (TuYV) in the model plant Arabidopsis thaliana also apply to the cultivated plant Camelina sativa, both belonging to the Brassicaceae family. In most experiments, we observed a significant increase in the relative emission of volatiles from TuYV-infected plants. Moreover, due to plant size, the global amounts of volatiles emitted by C. sativa were higher than those released by A. thaliana. In addition, the volatiles released by TuYV-infected C. sativa attracted the TuYV vector Myzus persicae more efficiently than those emitted by non-infected plants. In contrast, no such preference was observed for A. thaliana. We propose that high amounts of volatiles rather than specific metabolites are responsible for aphid attraction to infected C. sativa. This study points out that the data obtained from the model pathosystem A. thaliana/TuYV cannot be straightforwardly extrapolated to a related plant species infected with the same virus.
Enhancing Online Patient Support through Health-Care Knowledge in Online Health Communities: A Descriptive Study
Authors: Chen Donghua , Zhang Runtong , Liu Kecheng , Hou Lei
Published: August 2018

Online health communities (OHCs) should utilize health-care knowledge for enhancing online patient support. To examine the use of existing OHCs to identify the challenges and strategies of enhancing online patients’ decision-making support, we conducted a descriptive study to evaluate the information availability, user availability and knowledge usability in 100 carefully-selected health-related websites. On the basis of criteria for effective OHCs, we used three evaluation instruments for health-care professionals to review and score the websites. Questionnaire results were examined from the perspective of information, user and knowledge support. Results corroborate that over 80% of the websites facilitate effective social functions, whereas only 33% provide health-care decision-making services to online patients. Approximately 46% of them satisfy four or five effective OHCs’ criteria. Three of them only offer the functions of patients’ charts and journals to support health data management. Although the existing OHCs are facilitated with good social interaction and support, only a few can assist patients in making effective health-care decisions, not to mention properly using health-care knowledge support.
Dual Generalized Nonnegative Normal Neutrosophic Bonferroni Mean Operators and Their Application in Multiple Attribute Decision Making
Authors: Mo Jiongmei , Huang Han-Liang
Published: August 2018

For multiple attribute decision making, ranking and information aggregation problems are increasingly receiving attention. In a normal neutrosophic number, the ranking method does not satisfy the ranking principle. Moreover, the proposed operators do not take into account the correlation between any aggregation arguments. In order to overcome the deficiencies of the existing ranking method, based on the nonnegative normal neutrosophic number, this paper redefines the score function, the accuracy function, and partial operational laws. Considering the correlation between any aggregation arguments, the dual generalized nonnegative normal neutrosophic weighted Bonferroni mean operator and dual generalized nonnegative normal neutrosophic weighted geometric Bonferroni mean operator were investigated, and their properties are presented. Here, these two operators are applied to deal with a multiple attribute decision making problem. Example results show that the proposed method is effective and superior.
Outcome Prediction of Acute Kidney Injury Biomarkers at Initiation of Dialysis in Critical Units
Authors: Wu Vin-Cent , Shiao Chih-Chung , Chi Nai-Hsin , Wang Chih-Hsien , Chueh Shih-Chieh Jeff , Liou Hung-Hsiang , Spapen Herbert D. , Honore Patrick M. , Chu Tzong-Shinn
Published: August 2018

The ideal circumstances for whether and when to start RRT remain unclear. The outcome predictive ability of acute kidney injury (AKI) biomarkers measuring at dialysis initializing need more validation. This prospective, multi-center observational cohort study enrolled 257 patients with AKI undergoing renal replacement therapy (RRT) shortly after admission. At the start of RRT, blood and urine samples were collected for relevant biomarker measurement. RRT dependence and all-cause mortality were recorded up to 90 days after discharge. Areas under the receiver operator characteristic (AUROC) curves and a multivariate generalized additive model were applied to predict outcomes. One hundred and thirty-five (52.5%) patients died within 90 days of hospital discharge. Plasma c-terminal FGF-23 (cFGF-23) had the best discriminative ability (AUROC, 0.687) as compared with intact FGF-23 (iFGF-23) (AUROC, 0.504), creatinine-adjusted urine neutrophil gelatinase-associated lipocalin (AUROC, 0.599), and adjusted urine cFGF-23 (AUROC, 0.653) regardless whether patients were alive or not on day 90. Plasma cFGF-23 levels above 2050 RU/mL were independently associated with higher 90-day mortality (HR 1.76, p = 0.020). Higher cFGF-23 levels predicted less weaning from dialysis in survivors (HR, 0.62, p = 0.032), taking mortality as a competing risk. Adding cFGF-23 measurement to the AKI risk predicting score significantly improved risk stratification and 90-day mortality prediction (total net reclassification improvement = 0.148; p = 0.002). In patients with AKI who required RRT, increased plasma cFGF-23 levels correlated with higher 90-day overall mortality after discharge and predicted worse kidney recovery in survivors. When coupled to the AKI risk predicting score, cFGF-23 significantly improved mortality risk prediction. This observation adds evidence that cFGF-23 could be used as an optimal timing biomarker to initiate RRT.
Semantic Segmentation of Underwater Imagery Using Deep Networks Trained on Synthetic Imagery
Authors: O’Byrne Michael , Pakrashi Vikram , Schoefs Franck , Ghosh Bidisha
Published: August 2018

Recent breakthroughs in the computer vision community have led to the emergence of efficient deep learning techniques for end-to-end segmentation of natural scenes. Underwater imaging stands to gain from these advances, however, deep learning methods require large annotated datasets for model training and these are typically unavailable for underwater imaging applications. This paper proposes the use of photorealistic synthetic imagery for training deep models that can be applied to interpret real-world underwater imagery. To demonstrate this concept, we look at the specific problem of biofouling detection on marine structures. A contemporary deep encoder–decoder network, termed SegNet, is trained using 2500 annotated synthetic images of size 960 × 540 pixels. The images were rendered in a virtual underwater environment under a wide variety of conditions and feature biofouling of various size, shape, and colour. Each rendered image has a corresponding ground truth per-pixel label map. Once trained on the synthetic imagery, SegNet is applied to segment new real-world images. The initial segmentation is refined using an iterative support vector machine (SVM) based post-processing algorithm. The proposed approach achieves a mean Intersection over Union (IoU) of 87% and a mean accuracy of 94% when tested on 32 frames extracted from two distinct real-world subsea inspection videos. Inference takes several seconds for a typical image.
Wave and Tidal Controls on Embayment Circulation and Headland Bypassing for an Exposed, Macrotidal Site
Authors: McCarroll R. Jak , Masselink Gerd , Valiente Nieves G. , Scott Tim , King Erin V. , Conley Daniel
Published: August 2018

Headland bypassing is the transport of sediment around rocky headlands by wave and tidal action, associated with high-energy conditions and embayment circulation (e.g., mega-rips). Bypassing may be a key component in the sediment budget of many coastal cells, the quantification of which is required to predict the coastal response to extreme events and future coastal change. Waves, currents, and water levels were measured off the headland of a sandy, exposed, and macrotidal beach in 18-m and 26-m depths for 2 months. The observations were used to validate a Delft3D morphodynamic model, which was subsequently run for a wide range of scenarios. Three modes of bypassing were determined: (i) tidally-dominated control during low–moderate wave conditions [flux O (0–102 m3 day−1)]; (ii) combined tidal- and embayment circulation controls during moderate–high waves [O (103 m3 day−1)]; and (iii) multi-embayment circulation control during extreme waves [O (104 m3 day−1)]. A site-specific bypass parameter is introduced, which accurately (R2 = 0.95) matches the modelled bypass rates. A 5-year hindcast predicts bypassing is an order of magnitude less than observed cross-shore fluxes during extreme events, suggesting that bypassing at this site is insignificant at annual timescales. This work serves a starting point to generalise the prediction of headland bypassing.
Gene-Metabolite Interaction in the One Carbon Metabolism Pathway: Predictors of Colorectal Cancer in Multi-Ethnic Families
Authors: Shiao S. Pamela K. , Grayson James , Yu Chong Ho
Published: August 2018

For personalized healthcare, the purpose of this study was to examine the key genes and metabolites in the one-carbon metabolism (OCM) pathway and their interactions as predictors of colorectal cancer (CRC) in multi-ethnic families. In this proof-of-concept study, we included a total of 30 participants, 15 CRC cases and 15 matched family/friends representing major ethnic groups in southern California. Analytics based on supervised machine learning were applied, with the target variable being specified as cancer, including the ensemble method and generalized regression (GR) prediction. Elastic Net with Akaike’s Information Criterion with correction (AICc) and Leave-One-Out cross validation GR methods were used to validate the results for enhanced optimality, prediction, and reproducibility. The results revealed that despite some family members sharing genetic heritage, the CRC group had greater combined gene polymorphism-mutations than the family controls (p < 0.1) for five genes including MTHFR C677T, MTHFR A1298C, MTR A2756G, MTRR A66G, and DHFR 19bp. Blood metabolites including homocysteine (7 µmol/L), methyl-folate (40 nmol/L) with total gene mutations (≥4); age (51 years) and vegetable intake (2 cups), and interactions of gene mutations and methylmalonic acid (MMA) (400 nmol/L) were significant predictors (all p < 0.0001) using the AICc. The results were validated by a 3% misclassification rate, AICc of 26, and >99% area under the receiver operating characteristic curve. These results point to the important roles of blood metabolites as potential markers in the prevention of CRC. Future intervention studies can be designed to target the ways to mitigate the enzyme-metabolite deficiencies in the OCM pathway to prevent cancer.
Enif-Lang: A Specialized Language for Programming Network Functions on Commodity Hardware
Authors: Bonelli Nicola , Giordano Stefano , Procissi Gregorio
Published: August 2018

The maturity level reached by today’s commodity platforms makes even low-cost PCs viable alternatives to dedicated hardware to implement real network functions without sacrificing performance. Indeed, the availability of multi-core processing packages and multi-queue network interfaces that can be managed by accelerated I/O frameworks, provides off-the-shelf servers with the necessary power capability for running a broad variety of network applications with near hardware-class performance. At the same time, the introduction of the Software Defined Networks (SDN) and the Network Functions Virtualization (NFV) paradigms call for new programming abstractions and tools to allow this new class of network devices to be flexibly configured and functionally repurposed from the network control plane. The paper presents the ongoing work towards Enif-Lang (Enhanced Network processIng Functional Language), a functional language for programming network functions over generic middleboxes running the Linux operating system. The language addresses concurrent programming by design and is targeted at developing simple stand-alone applications as well as pre-processing stages of packet elaborations. Enif-Lang is implemented as a Domain Specific Language embedded in the Haskell language and inherits the main principles of its ancestor, including the strong typedness and the concept of function compositions. Complex network functions are implemented by composing a set of elementary operations (primitives) by means of a compact yet expressive language grammar. Throughout the paper, the description of the design principles and features of Enif-Lang are accompanied by examples and use cases. In addition, a preliminary performance assessment is carried out to prove the effectiveness of the language for developing practical applications with the performance level required by 5G systems and the Tactile Internet.
Combustion Behaviors of CIGS Thin-Film Solar Modules from Cone Calorimeter Tests
Authors: Yin Lulu , Jiang Yong , Qiu Rong
Published: August 2018

As is well known, building integrated photovoltaic (BIPV) technology is becoming more commonly used in residential and commercial buildings. Fire assessment of photovoltaic (PV) modules as a whole is still insufficient. This work focuses on the thermal properties and combustion behavior of CIGS (copper, indium, gallium and selenium) thin-film modules. Cone calorimeter experiments were conducted at different external heat flux of 25, 30, 35, 40 and 45 kW m−2. Several parameters are discussed, including surface temperature, ignition time, heat release rate (HRR), mass loss rate, carbon monoxide (CO) and carbon dioxide (CO2) concentrations. The results show that CIGS thin-film solar modules are inflammable at intermediate or high flashover risk. A correction calculation for the gas toxicity index has been used to reduce the well-ventilation condition effect. Compared with the uncorrected calculation, peak fractional effective dose (FED) and lethal concentration for 50% of the population (LC50) are almost double. This work will help to determine a more stringent fire safety provision for PV modules.
Mechanical Characteristics of Hybrid Composites with ±45° Glass and 0°/90° Stainless Steel Fibers
Authors: O′Brien Caitlin , Zaghi Arash E.
Published: August 2018

Lack of energy dissipation is one of the shortcomings of conventional glass fiber reinforced composites. The addition of steel fibers to the conventional FRP composite to create a hybrid composite has been recently investigated as an option to address this limitation. The current literature is limited to composites reinforced with metal and non-metal fibers of the same alignment. In this study, hybrid and nonhybrid FRP composites of different layups, fiber content, and weave type were manufactured and subjected to hysteretic tensile loads. Woven glass fabrics in ±45° orientation were hybridized with unidirectional stainless steel fabrics in 0° and 90° orientations. This put the glass and steel layers in in-plane shear and normal stresses, respectively. The nonlinear stress–strain relationship, residual plastic strains, energy dissipation capability, and failure mechanisms of hybrid and nonhybrid composite type were compared. The hybrid composites presented improved energy dissipation, tensile strength, and stiffness when compared to nonhybrid ones. The applicability of an existing constitutive model that was originally developed for in-plane shear of conventional composites was investigated and refinements were proposed to present the hysteretic stress–strain relationship after addition of steel fibers. The refined model captured the increased plastic strain values and energy dissipation because of stainless steel fibers in the hybrid composite samples. An Armstrong–Frederick plasticity model was implemented to model the stress–strain relationship of the stainless steel composite samples.
Radial Compressive Property and the Proof-of-Concept Study for Realizing Self-expansion of 3D Printing Polylactic Acid Vascular Stents with Negative Poisson’s Ratio Structure
Authors: Wu Zichao , Zhao Ji , Wu Wenzheng , Wang Peipei , Wang Bofan , Li Guiwei , Zhang Shuo
Published: August 2018

Biodegradable stents offer the potential to reduce the in-stent restenosis by providing support long enough for the vessel to heal. The polylactic acid (PLA) vascular stents with negative Poisson’s ratio (NPR) structure were manufactured by fused deposition modeling (FDM) 3D printing in this study. The effects of stent diameter, wall thickness and geometric parameters of arrowhead NPR structure on radial compressive property of 3D-printed PLA vascular stent were studied. The results showed that the decrease of stent diameter, the increase of wall thickness and the increase of the surface coverage could enhance the radial force (per unit length) of PLA stent. The radial and longitudinal size of PLA stent with NPR structure decreased simultaneously when the stent was crimped under deformation temperature. The PLA stent could expand in both radial and longitudinal direction under recovery temperature. When the deformation temperature and recovery temperature were both 65 °C, the diameter recovery ratio of stent was more than 95% and the maximum could reach 98%. The length recovery ratio was above 97%. This indicated the feasibility of utilizing the shape memory effect (SME) of PLA to realize the expansion of 3D-printed PLA vascular stent under temperature excitation.
POSS Nanofiller-Induced Enhancement of the Thermomechanical Properties in a Fluoroelastomer Terpolymer
Authors: Berthier Daphné , Deffarges Marie-Pierre , Berton Nicolas , Venin Mathieu , Lacroix Florian , Schmaltz Bruno , Tendron Yohan , Pestel Eric , Tran-Van François , Méo Stéphane
Published: August 2018

The present study reports on the use of three types of polyhedral oligomeric silsesquioxanes (POSS) nanoparticles with various organic substituents as fillers in a fluoroelastomer (FKM). A series of/POSS elastomer composite thin films is prepared. Microstructural SEM/TEM (scanning electron microscopy/transmission electron microscopy) imaging reveals a dispersion state allowing the presence of micron-sized domains. The influence of POSS content is studied in order to optimize thermal stability and mechanical properties of the composite thin films. Both POSS-A (with an acryloyl functional group and seven isobutyl substituents) and POSS-P (with eight phenyl substituents) lead to higher thermal stability and modulus of the composites, with respect to the unfilled FKM terpolymer matrix. covalent grafting of POSS-A onto the FKM network is found to play a critical role. Enhanced storage modulus in the rubbery plateau region (+210% at 200 °C for 20 phr) suggests that POSS-A is particularly suitable for high temperature applications.
The Effect of the Morphology of Coarse Aggregate on the Properties of Self-Compacting High-Performance Fibre-Reinforced Concrete
Authors: Ostrowski Krzysztof , Sadowski Łukasz , Stefaniuk Damian , Wałach Daniel , Gawenda Tomasz , Oleksik Konrad , Usydus Ireneusz
Published: August 2018

When understanding the effect of the morphology of coarse aggregate on the properties of a fresh concrete mixture, the strength and deformability of self-compacting high-performance fibre-reinforced concrete (SCHPFRC) can be seen to be critical for its performance. In this research, regular and irregular grains were separated from granite coarse aggregate. The morphology of these grains was described while using digital image analysis. As a result, the aspect ratio, roundness and area ratio were determined in order to better understand this phenomenon. Then, the principal rheological, physical, and mechanical properties of SCHPFRC were determined. The obtained results indicated that the morphology of the grains of coarse aggregate has an impact on the strength and stiffness properties of SCHPFRC. Moreover, significant differences in the transverse strain of concretes were observed. The morphology of the coarse aggregate also has an impact on the rheological parameters of a fresh concrete mixture. To better understand this phenomenon, the hypothesized mechanism of the formation of SCHPFRC caused by different morphology of coarse aggregate was proposed at the end of the article.
Injectable Hyaluronic Acid-co-Gelatin Cryogels for Tissue-Engineering Applications
Authors: Rezaeeyazdi Mahboobeh , Colombani Thibault , Memic Adnan , Bencherif Sidi A.
Published: August 2018

Polymeric scaffolds such as hydrogels can be engineered to restore, maintain, or improve impaired tissues and organs. However, most hydrogels require surgical implantation that can cause several complications such as infection and damage to adjacent tissues. Therefore, developing minimally invasive strategies is of critical importance for these purposes. Herein, we developed several injectable cryogels made out of hyaluronic acid and gelatin for tissue-engineering applications. The physicochemical properties of hyaluronic acid combined with the intrinsic cell-adhesion properties of gelatin can provide suitable physical support for the attachment, survival, and spreading of cells. The physical characteristics of pure gelatin cryogels, such as mechanics and injectability, were enhanced once copolymerized with hyaluronic acid. Reciprocally, the adhesion of 3T3 cells cultured in hyaluronic acid cryogels was enhanced when formulated with gelatin. Furthermore, cryogels had a minimal effect on bone marrow dendritic cell activation, suggesting their cytocompatibility. Finally, in vitro studies revealed that copolymerizing gelatin with hyaluronic acid did not significantly alter their respective intrinsic biological properties. These findings suggest that hyaluronic acid-co-gelatin cryogels combined the favorable inherent properties of each biopolymer, providing a mechanically robust, cell-responsive, macroporous, and injectable platform for tissue-engineering applications.