bluuebunny/crossref_metadata_2025 huggingface dataset:
Addressing maternal medication use during breastfeeding using clinical resources and a novel physiologically based pharmacokinetic model-derived metric: A qualitative study
Authors: Yeung Cindy Hoi Ting , Houle Sherilyn K. D. , Anderson Philip O. , Best Brookie M. , Dubinsky Samuel , Edginton Andrea N.
Published: April 2023

Introduction

Breastfeeding has major benefits to the maternal-infant dyad and yet healthcare providers have expressed uncertainty about advocating breastfeeding when mothers are taking medications. The tendency for some providers to be more cautious in their advising approach is likely a consequence of limited, unfamiliar, and unreliable existing information on medication use during lactation. A novel risk metric termed the Upper Area Under the Curve Ratio (UAR) was developed to overcome existing resource shortcomings. However, the perception and use of the UAR in practice by providers is not known. The aim of this study was to understand existing resource use and potential UAR use in practice, their advantages and disadvantages, and areas of improvement for the UAR.

Methods

Healthcare providers mainly practicing in California with experience advising on medication use during lactation were recruited. One-on-one semi-structured interviews that included questions on current practices when advising medication use during breastfeeding, and approaches to a given a scenario with and without information about the UAR were conducted. The Framework Method was applied for data analysis to construct themes and codes.

Results

Twenty-eight providers representing multiple professions and disciplines were interviewed. Six main themes emerged: (1) Current Practice Approaches, (2) Advantages of Existing Resources, (3) Disadvantages of Existing Resources, (4) Advantages of the UAR, (5) Disadvantages of the UAR, and (6) Strategies to Improve the UAR. Overall, 108 codes were identified that illustrated theme topics ranging from a general lack of metric use to the realities of advising. A workflow describing current practice approaches connected all other themes. Almost all disadvantages of existing resources could be overcome by advantages of other resources and the UAR. Several improvements to the UAR were identified to address its shortcomings.

Conclusion

Through interviews with providers who use resources to advise on medication use during breastfeeding, an improved understanding of current practice approaches and accessed resources was ascertained. Ultimately, it was found that the UAR would confer multiple benefits over existing resources, and improvements of the UAR were identified. Future work should focus on implementing the suggested recommendations to ensure optimal uptake of the UAR to improve advising practices.
Development of a nomogram model to predict malignant vasovagal syncope in Chinese children
Authors: Sun Rui , Kang Yingying , Zhang Mingming , Wang Hongmao , Shi Lin , Li Xiaohui
Published: April 2023

Objective

Vasovagal syncope (VVS) is the commonest form of syncope, and malignant VVS draws substantial attention due to its life-threatening cardiac asystolic risk. This study aimed to explore the predictive role of a wide panel of clinical indicators for malignant VVS in children, and further to develop a nomogram model.

Methods

This is a retrospective case-control study. VVS is diagnosed based on head-up tilt test (HUTT). STATA software (version 14.0) was used for statistical analysis, and effect sizes are expressed as odds ratio (OR) and 95% confidence interval (CI).

Results

Total 370 children with VVS were analyzed, and of them 16 children had malignant VVS. Sixteen malignant VVS and 64 non-malignant VVS were matched on age and sex by a 1:4 propensity scores matching method. Mean corpuscular hemoglobin (MCH) and standard deviation of average RR intervals milliseconds (SDANN) were significantly and independently associated with malignant VVS after adjusting for confounders, with OR reaching 1.437 (95% CI: 1.044 to 1.979; P = 0.026) and 1.035 (95% CI: 1.003 to 1.068; P = 0.029), respectively. Calibration and discrimination analyses revealed that the addition of MCH and SDANN can enhance model performance. Then, a nomogram to predict malignant VVS was developed using general characteristics and two above significant factors, and higher values in medical history, number of syncope, MCH and SDANN were associated with a greater risk of malignant VVS.

Conclusion

MCH and SDANN were two promising factors for the development of malignant VVS, and modeling of significant factors in a nomogram can provide strong reference to aid clinical decision-making.
Geniposide plus chlorogenic acid reverses non-alcoholic steatohepatitis via regulation of gut microbiota and bile acid signaling in a mouse model in vivo
Authors: Li Hongshan , Xi Yingfei , Xin Xin , Feng Qin , Hu Yiyang
Published: April 2023

Background: Geniposide and chlorogenic acid are the major active ingredients in Yinchenhao Decoction and are widely used as herbal medicines in Asia. This study further assessed their effects on improvement of non-alcoholic steatohepatitis (NASH) in a mouse model and explored the underlying molecular events in vivo.

Methods: Male C57BL/6 and farnesoid X receptor knockout (FXR−/−) mice were used to establish the NASH model and were treated with or without geniposide, chlorogenic acid, obeticholic acid (OCA), and antibiotics for assessment of the serum and tissue levels of various biochemical parameters, bile acid, DNA sequencing of bacterial 16S amplicon, protein expression, and histology.

Results: The data showed that the combination of geniposide and chlorogenic acid (GC) reduced the levels of blood and liver lipids, serum alanine aminotransferase (ALT), serum aspartate aminotransferase (AST), and the liver tissue index in NASH mice. In addition, GC treatment improved the intestinal microbial disorders in the NASH mice as well as the intestinal and serum bile acid metabolism. At the gene level, GC induced FXR signaling, i.e., increased the expression of FXR, small heterodimer partner (SHP), and bile salt export pump (BSEP) in liver tissues and fibroblast growth factor 15 (FGF15) expression in the ileal tissues of NASH mice. However, antibiotics (ampicillin, neomycin, vancomycin, and tinidazole) in drinking water (ADW) reversed the effect of GC on NASH and altered the gut microbiota in NASH mice in vivo. Furthermore, GC treatment failed to improve NASH in the FXR−/− mouse NASH model in vivo, indicating that the effectiveness of GC treatment might be through FXR signaling activation.

Conclusion: GC was able to alleviate NASH by improving the gut microbiome and activating FXR signaling; its effect was better than each individual agent alone.
Potential therapeutic effects of Chinese meteria medica in mitigating drug-induced acute kidney injury
Authors: Li Jun , Li Tonglu , Li Zongping , Song Zhiyong , Gong Xuezhong
Published: April 2023

Drug-induced acute kidney injury (DI-AKI) is one of the leading causes of kidney injury, is associated with high mortality and morbidity, and limits the clinical use of certain therapeutic or diagnostic agents, such as antineoplastic drugs, antibiotics, immunosuppressants, non-steroidal anti-inflammatory drugs, and contrast media. In recent years, numerous studies have shown that many Chinese meteria medica, metabolites derived from botanical drugs, and Chinese medicinal formulas confer protective effects against DI-AKI by targeting a variety of cellular or molecular mechanisms, such as oxidative stress, inflammatory, cell necrosis, apoptosis, and autophagy. This review summarizes the research status of common DI-AKI with Chinese meteria medica interventions, including cisplatin, gentamicin, contrast agents, methotrexate, and acetaminophen. At the same time, this review introduces the metabolites with application prospects represented by ginseng saponins, tetramethylpyrazine, panax notoginseng saponins, and curcumin. Overall, this review provides a reference for the development of promising nephroprotectants.
Integrative bioinformatics approaches to establish potential prognostic immune-related genes signature and drugs in the non-small cell lung cancer microenvironment
Authors: Zhou Jiao , Shi Shan , Qiu Yeqing , Jin Zhongwen , Yu Wenyan , Xie Rongzhi , Zhang Hongyu
Published: April 2023

Introduction: Research has revealed that the tumor microenvironment (TME) is associated with the progression of malignancy. The combination of meaningful prognostic biomarkers related to the TME is expected to be a reliable direction for improving the diagnosis and treatment of non-small cell lung cancer (NSCLC).

Method and Result: Therefore, to better understand the connection between the TME and survival outcomes of NSCLC, we used the “DESeq2” R package to mine the differentially expressed genes (DEGs) of two groups of NSCLC samples according to the optimal cutoff value of the immune score through the ESTIMATE algorithm. A total of 978 up-DEGs and 828 down-DEGs were eventually identified. A fifteen-gene prognostic signature was established via LASSO and Cox regression analysis and further divided the patients into two risk sets. The survival outcome of high-risk patients was significantly worse than that of low-risk patients in both the TCGA and two external validation sets (p-value < 0.05). The gene signature showed high predictive accuracy in TCGA (1-year area under the time-dependent ROC curve (AUC) = 0.722, 2-year AUC = 0.708, 3-year AUC = 0.686). The nomogram comprised of the risk score and related clinicopathological information was constructed, and calibration plots and ROC curves were applied, KEGG and GSEA analyses showed that the epithelial-mesenchymal transition (EMT) pathway, E2F target pathway and immune-associated pathway were mainly involved in the high-risk group. Further somatic mutation and immune analyses were conducted to compare the differences between the two groups. Drug sensitivity provides a potential treatment basis for clinical treatment. Finally, EREG and ADH1C were selected as the key prognostic genes of the two overlapping results from PPI and multiple Cox analyses. They were verified by comparing the mRNA expression in cell lines and protein expression in the HPA database, and clinical validation further confirmed the effectiveness of key genes.

Conclusion: In conclusion, we obtained an immune-related fifteen-gene prognostic signature and potential mechanism and sensitive drugs underling the prognosis model, which may provide accurate prognosis prediction and available strategies for NSCLC.
Enhanced external counterpulsation modulates the heartbeat evoked potential
Authors: Liu Hongyun , Liang Hui , Yu Xiaohua , Wang Guojing , Han Yi , Yan Muyang , Li Shijun , Wang Weidong
Published: April 2023

Introduction: Accumulating evidence suggests that enhanced external counterpulsation (EECP) influences cardiac functions, hemodynamic characteristics and cerebral blood flow. However, little is known about whether or how the EECP affects the brain-heart coupling to produce these physiological and functional changes. We aimed to determine if the brain-heart coupling is altered during or after EECP intervention by assessing the heartbeat evoked potential (HEP) in healthy adults.

Methods: Based on a random sham-controlled design, simultaneous electroencephalography and electrocardiography signals as well as blood pressure and flow status data were recorded before, during and after two consecutive 30-min EECP in 40 healthy adults (female/male: 17/23; age: 23.1 ± 2.3 years). HEP amplitude, frequency domain heart rate variability, electroencephalographic power and hemodynamic measurements of 21 subjects (female/male: 10/11; age: 22.7 ± 2.1 years) receiving active EECP were calculated and compared with those of 19 sham control subjects (female/male: 7/12; age: 23.6 ± 2.5 years).

Results: EECP intervention caused immediate obvious fluctuations of HEP from 100 to 400 ms after T-peak and increased HEP amplitudes in the (155–169) ms, (354–389) ms and (367–387) ms time windows after T-peak in the region of the frontal pole lobe. The modifications in HEP amplitude were not associated with changes in the analyzed significant physiological measurements and hemodynamic variables.

Discussion: Our study provides evidence that the HEP is modulated by immediate EECP stimuli. We speculate that the increased HEP induced by EECP may be a marker of enhanced brain-heart coupling. HEP may serve as a candidate biomarker for the effects and responsiveness to EECP.
Characteristics of historical precipitation for winter wheat cropping in the semi-arid and semi-humid area
Authors: Fang Dan , Huang Jingyao , Sun Weiwei , Ullah Najeeb , Jin Suwen , Song Youhong
Published: April 2023

Winter wheat (Triticum aestivum L.) is one of major crops in the area along Huai river, China where it is a semi-arid and semi-humid region with sufficient precipitation for an entire season, but with uneven distribution within various growth stages. The instability of precipitation is an important factor in limiting wheat production potential under climate change. Therefore, it is essential to characterise the precipitation associated with different crop developmental stages. Based on climate data from 1999 to 2020 in six representative meteorological stations, we characterised the historical precipitation relating to seven key growth stages in winter wheat. There is no clear trend of interannual variation of precipitation for wheat season, with an average of precipitation of 414.4 ± 121.2 mm. In terms of the distribution of precipitation grade within a season, light rain was dominant. Continuous rain occurred frequently during the pre-winter seedling and overwintering stages. The critical period of water demand, such as jointing and booting, has less precipitation. The fluctuation range of precipitation in sowing, heading-filling and maturation stages is large, which means that there is flood and drought at times. In conclusion, these findings provide a foundation for instructing winter wheat cropping in confronting with waterlogging and drought risk due to uneven precipitation in ‘Yanhuai’ region, China.
Crop pest image classification based on improved densely connected convolutional network
Authors: Peng Hongxing , Xu Huiming , Gao Zongmei , Zhou Zhiyan , Tian Xingguo , Deng Qianting , He Huijun , Xian Chunlong
Published: April 2023

Introduction

Crop pests have a great impact on the quality and yield of crops. The use of deep learning for the identification of crop pests is important for crop precise management.

Methods

To address the lack of data set and poor classification accuracy in current pest research, a large-scale pest data set named HQIP102 is built and the pest identification model named MADN is proposed. There are some problems with the IP102 large crop pest dataset, such as some pest categories are wrong and pest subjects are missing from the images. In this study, the IP102 data set was carefully filtered to obtain the HQIP102 data set, which contains 47,393 images of 102 pest classes on eight crops. The MADN model improves the representation capability of DenseNet in three aspects. Firstly, the Selective Kernel unit is introduced into the DenseNet model, which can adaptively adjust the size of the receptive field according to the input and capture target objects of different sizes more effectively. Secondly, in order to make the features obey a stable distribution, the Representative Batch Normalization module is used in the DenseNet model. In addition, adaptive selection of whether to activate neurons can improve the performance of the network, for which the ACON activation function is used in the DenseNet model. Finally, the MADN model is constituted by ensemble learning.

Results

Experimental results show that MADN achieved an accuracy and F1Score of 75.28% and 65.46% on the HQIP102 data set, an improvement of 5.17 percentage points and 5.20 percentage points compared to the pre-improvement DenseNet-121. Compared with ResNet-101, the accuracy and F1Score of MADN model improved by 10.48 percentage points and 10.56 percentage points, while the parameters size decreased by 35.37%. Deploying models to cloud servers with mobile application provides help in securing crop yield and quality.
The effects of anxiety on practice behaviors and performance quality in expert pianists
Authors: Passarotto Edoardo , Worschech Florian , Altenmüller Eckart
Published: April 2023

Introduction

During their career, musicians need to undergo intense periods of training to master musical instruments and become accomplished artists. Dysfunctional practice behaviors and anxiety are often mentioned among the possible risk factors for playing-related injuries in musicians. However, the mechanism through which these might lead to the onset of these injuries is still unclear. The present study aims at overcoming this limitation by investigating the relationship between quantitative measurements of anxiety, practice behaviors and music performance quality.

Methods

The experiment consisted in monitoring practice behaviors in 30 pianists practicing a short musical task.

Results

Most self-report anxiety measurements were positively correlated with practice time, especially those collected right before the practice sessions. Similar correlations were identified between anxiety and the number of repetitions of the musical task. Physiological markers of anxiety were only weakly related to practice behaviors. Subsequent analyses showed that high levels of anxiety were associated with poor quality of music performances at baseline. Nevertheless, the interaction between participants’ learning rate and anxiety measures showed no association with performance quality scores. Moreover, anxiety and performance quality co-developed during practice sessions, showing that pianists who improved their playing were also less anxious in the latter part of the experiment.

Discussion

These findings suggest that anxious musicians are likely at higher risk of developing playing-related injuries related to overuse and repetitive strains. Future directions and clinical implications are discussed.
Time trends and heterogeneity in the disease burden of visual impairment due to cataract, 1990–2019: A global analysis
Authors: Han Xiaotong , Zou Minjie , Liu Zhenzhen , Sun Yi , Young Charlotte Aimee , Zheng Danying , Jin Guangming
Published: April 2023

Objectives

This study aimed to estimate the disease burden of cataract and evaluate the contributions of risk factors to cataract-associated disability-adjusted life years (DALYs).

Materials and methods

Prevalence and DALYs of visual impairment due to cataract were extracted from the Global Burden of Disease (GBD) study 2019 to explore time trends and annual changes. Regional and country-level socioeconomic indexes were obtained from open databases. The time trend of prevalence and DALYs was demonstrated. Stepwise multiple linear regression was used to evaluate associations between the age-standardized rate of DALYs of cataract and potential predictors.

Results

Global Prevalence rate of visual impairment due to cataract rose by 58.45% to 1,253.9 per 100,000 population (95% CI: 1,103.3 to 1,417.7 per 100,000 population) in 2019 and the DALYs rate rose by 32.18% from 65.3 per 100,000 population (95% CI: 46.4 to 88.2 per 100,000 population) in 1990 to 86.3 per 100,000 population (95% CI: 61.5 to 116.4 per 100,000 population) in 2019. Stepwise multiple linear regression model showed that higher refractive error prevalence (β = 0.036, 95% CI: 0.022, 0.050, P < 0.001), lower number of physicians per 10,000 population (β = −0.959, 95% CI: −1.685, −0.233, P = 0.010), and lower level of HDI (β = −134.93, 95% CI: −209.84, −60.02, P = 0.001) were associated with a higher disease burden of cataract.

Conclusion

Substantial increases in the prevalence of visual impairment and DALYs of cataract were observed from 1990 to 2019. Successful global initiatives targeting improving cataract surgical rate and quality, especially in regions with lower socioeconomic status, is a prerequisite to combating this growing burden of cataract in the aging society.
Relay intercropping of maize with common dry beans to rationalize nitrogen fertilizer
Authors: El-Mehy Amira A. , Shehata Manal A. , Mohamed Ahmed S. , Saleh Said A. , Suliman Ahmed A.
Published: April 2023

Maize (Zea mays L.) and dry beans (Phaseolus vulgaris L.) are important staple food and cash crops worldwide. Common bean in an intercrop with maize contributes to biological nitrogen fixation, which stabilize productivity of cropping systems and reduce negative environmental impacts and loss of biodiversity for sustainable agriculture. A field experiments was performed during the years of 2020 and 2021 at Sers El-Layian Station, northern Egypt. The current study aiming to study the effect of three sowing dates of maize, represent 3 co-growth duration [T1: at flowering stage (FS) of common beans (60 days co-growth duration), T2: 15 days after FS (45 days co-growth duration), and T3: 30 days after FS (30 days co-growth duration with beans)] and three N fertilizer levels (N1: 190.4, N2: 238.0, and N3: 285.6 kg N/ha of maize) on productivity, profitability and N fertilizer rationalization. The longest co-growth duration of maize intercropping with common beans (T1) significantly (P ≤ 0.05) decreased common beans and maize yields compared with T2 and T3. Performance of common beans did not show (P ≤ 0.05) any variation under different N fertilizer levels of maize. Significant (P ≤ 0.05) increase in maize yield and its components with raising N fertilizer level up to N3. Although there was no significant variation in maize yield when applied N2 and N3, however, nitrogen use efficiency (NUE) was significant (P ≤ 0.05) higher in N2 than N3 by 18.34%. Regardless of planting time and N fertilizer level of maize, combined productivity of common beans and maize increased in the intercropped system as cleared by higher total land equivalent ratios (LER) and area time equivalent ratios (ATER). Highest LER value 1.99 was observed at the shortest co-growth period T3 under N3 followed by 1.97 with N2. Positive values in the actual yield loss index (AYL) indicated intercropping advantage. Different competition indices showed a greater dominance of maize over common beans (aggressivity, Ag; competitive ratio, CR; actual yield losses, AYL). However, the intercropping systems increased the economic advantage (intercropping advantage index, IAI and monetary advantage index MAI) over monoculture. These results imply that shortening the period of co-growth maize with common beans (T3) and applying 238.0 kg N/ha in the relay intercropping system reduced mineral N fertilizer use by 16.67% compared to the advised level 285.6 kg N/ha along with increased productivity per unit area and economic advantages for small-farmer.
A coupled and coordinated analysis in livelihoods of poverty alleviation migrants from a just transition perspective
Authors: Tao Siji , Shi Guoqing
Published: April 2023

Introduction

From 2016 to 2020, China resettled 9.6 million poor people for the purpose of poverty alleviation. While this policy achieved the goal of eliminating absolute poverty, it also caused massive displacement and problems related to achieving a “just transition”. This study considers the “coupled coordination model” of resettlement, and examines livelihood capital and livelihood stability, from the perspective of “just transition”.

Methods

This study is based on survey data gathered in Lanping County, Yunnan Province from 235 resettled people that belong to “ethnic minority groups directly-entering-socialism”, making use of the livelihood capital assessment index system of farm households which is based on China's poverty alleviation resettlement policy and background-established a “coupled coordination degree model” which coordinates and combines livelihood capital and livelihood stability analyzes how accumulation of livelihood capital affects livelihood stability.

Results

Results indicate that (1) resettlement and supportive measures increased the coupling and coordination of migrants' household livelihood capital and livelihood stability.; (2) This type of coupled coordination led to a change from “dysfunctional decline type” to a “transitional development type”, thus reflecting “just transition”; (3) Although livelihood capital had an important positive impact on the coupling and coordination of migrants livelihood coupling and coordination, different livelihood strategies influenced livelihood coupling and coordination in different ways.

Discussion

This requires that government decision makers focus on facilitating a “just transition” as migrants become urbanized citizens. Disposing of natural resources such as farmland for migrants, promoting the implementation of land circulation policies, considering the contribution of different types of livelihood capital to different families, improving the precision of employment training, and promoting sustainable livelihood development.
Analgesic effect of subcutaneous injection of different concentrations of methylene blue after hemorrhoidectomy: A retrospective study
Authors: Long Qing , Li Jun , Li Yan
Published: April 2023

Objective

Subcutaneous injection of methylene blue around the anus may help reduce postoperative pain. However, the concentration of methylene blue is still controversial. Therefore, Our study aims to investigate the efficacy and safety of different methylene blue injected concentrations subcutaneously in pain treatment after hemorrhoidectomy.

Methods

A total of 180 consecutive patients with grade III or IV hemorrhoids from March 2020 to December 2021 were reviewed. All patients underwent hemorrhoidectomy under spinal anesthesia and were divided into three groups. Group A received subcutaneous injection of 0.1% methylene blue after hemorrhoidectomy, group B received subcutaneous injection of 0.2% methylene blue, and Group C did not received subcutaneous injection of methylene blue. The primary outcome measures were the visual analog scale (VAS) pain score on postoperative days 1, 2, 3, 7, 14, and total analgesic consumption within 14 days. Secondary outcomes were complications after hemorrhoidectomy, including acute urinary retention, secondary bleeding, perianal incision edema, and perianal skin infection, and the Wexner scores used to assess the level of anal incontinence at one and three months after surgery.

Results

There was no significant difference among three groups in sex, age, course of the disease, hemorrhoid grade and the number of incisions, and there was no significant difference in the volume of methylene blue injected between group A and group B. The VAS pain score and total analgesics consumption within 14 days in group A and group B were significantly lower than those in group C, but the differences between group A and group B were not statistically significant. The Wexner scores of group B were significantly higher than those of group A and group C one month after the operation, but the differences between group A and group C were not statistically significant. In addition, the Wexner score among three groups decreased to zero at three months after operation. There was no significant difference in the incidence of other complications among three groups.

Conclusion

The perianal injection of 0.1% methylene blue and 0.2% methylene blue have a similar analgesic effect in pain treatment after hemorrhoidectomy, but 0.1% methylene blue has higher safety.
Comparative analysis of newly identified rodent arteriviruses and porcine reproductive and respiratory syndrome virus to characterize their evolutionary relationships
Authors: Zhao Zhuang-Yan , Yu De , Ji Chun-Miao , Zheng Qiankun , Huang Yao-Wei , Wang Bin
Published: April 2023

Porcine reproductive and respiratory syndrome virus (PRRSV) has caused huge economic losses for the global pig industry, but its origins and evolution remain a mystery. In 2018, the genome sequences of seven arteriviruses isolated from rodents were determined, and here we publish new analysis showing that they may be ancestors of PRRSV. The sequence similarity of these viruses to PRRSV was ~60%, with shared genome organization and other characteristics, such as slippery sequences and C-rich motifs in nsp2, and a transactivated protein sequence in nsp1β. Codon usage basis analysis showed that PRRSV was closer to these rodent arteriviruses than lactate dehydrogenase-elevating virus (LDV) and they were both under pressure of natural selection. Evolutionary analysis revealed that four of the rodent arteriviruses shared the same genus with PRRSV, and were more closely related to PRRSV-2 than PRRSV-1. In addition to this, they all appeared earlier than PRRSV according to evolutionary modeling, and we speculate that they represent an intermediate step in the origin of PRRSV by arterivirus transmission from rodents to swine. Our in-depth analysis furthers our understanding of arteriviruses, and will serve as the basis for subsequent exploration of the evolution of PRRSV and other arteriviruses.
Case report: Limb-sparing surgery of tibial chondrosarcoma with frozen autologous bone graft using liquid nitrogen in a dog
Authors: Shimada Masakazu , Nagashima Tomokazu , Michishita Masaki , Yazawa Daisuke , Hara Yasushi
Published: April 2023

Chondrosarcoma is the second most common primary bone tumor after osteosarcoma in dogs. Chondrosarcoma has a good prognosis owing to its low metastatic rate and long survival time, even with amputation alone. However, amputation risks reducing the quality of life in patients with other orthopedic diseases of the non-affected limb, neurological diseases, or large body size. Limb-sparing surgery with frozen autologous bone grafting using liquid nitrogen allows bone quality to be maintained in the normal bone area while killing tumor cells, thereby preserving the affected limb. Thus, it is expected to maintain the quality of life. We describe herein limb-sparing surgery for tibial chondrosarcoma with frozen autologous bone graft using liquid nitrogen in an 8-year and 8-month-old castrated male bulldog weighing 29.2 kg. The patient had chondrosarcoma of the left tibia, suspected cranial cruciate ligament rupture of the right stifle, and degenerative lumbosacral stenosis. In such a case, amputation would increase the burden on the non-affected limb or spine, which could cause difficulty in walking; therefore, we performed limb-sparing surgery. Postoperatively, although a circumduction gait associated with stifle arthrodesis remained, the patient maintained the quality of life for 20 months, and the owner was satisfied with the results.
Pharmacist Interventions in Minimizing Drug Related Problems in Diabetes With Co-Existing Hypertension: A Five-Year Overview and Ground Report From India
Authors: Osoro Ian , Amir Mohammed , Vohra Manisha , Sharma Amit
Published: April 2023

Objective: The study aimed to investigate the pharmacist interventions in minimizing drug-related problems in diabetes with co-existing hypertension.

Methods: Prospective observational study.

Results: Overall, a total of 628 interventions were recommended for 1,914 patients during the 5-year period of study. Among all the interventions, the majority were suggested regarding “substituting the drug” (39%), change in frequency of administration (25%), and addition of drug (14%). Patient compliance status was found significant (p = 0.29 ± 0.07).

Conclusion: Clinical pharmacists have a crucial role in minimizing drug related problems. Particularly, there should be a greater emphasis on patient counselling and patient follow-up.
NSGA-PINN: A Multi-Objective Optimization Method for Physics-Informed Neural Network Training
Authors: Lu Binghang , Moya Christian , Lin Guang
Published: April 2023

This paper presents NSGA-PINN, a multi-objective optimization framework for the effective training of physics-informed neural networks (PINNs). The proposed framework uses the non-dominated sorting genetic algorithm (NSGA-II) to enable traditional stochastic gradient optimization algorithms (e.g., ADAM) to escape local minima effectively. Additionally, the NSGA-II algorithm enables satisfying the initial and boundary conditions encoded into the loss function during physics-informed training precisely. We demonstrate the effectiveness of our framework by applying NSGA-PINN to several ordinary and partial differential equation problems. In particular, we show that the proposed framework can handle challenging inverse problems with noisy data.
Distance Learning of Financial Accounting: Mature Undergraduate Students’ Perceptions
Authors: Maldonado Isabel , Silva Ana Paula , Magalhães Miguel , Pinho Carlos , Pereira Manuel Sousa , Torre Lígia
Published: April 2023

This research sought to explore self-reported satisfaction levels of mature students enrolled in the virtual financial accounting course of the first online-only bachelor’s degree in Portugal. While doing so, it attempted to generate understanding of which factors may affect undergraduate mature students’ engagement—herein measured in terms of overall satisfaction—with online learning, particularly, of financial accounting. Thereby, this research addresses several research gaps. First, unlike most recent empirical research, it provides evidence from a post-pandemic period, in 2022. Second, responding to calls for further education research in different contexts, Portugal poses a highly relevant, unexplored research setting since it was only in 2019 that the Portuguese government approved a legal regime to frame distance education at Higher Education Institutions (HEIs). Third, this research focuses on the overlooked, and yet growing, population of adult mature students. The research evidence emerges from 32 valid responses to a structured electronic questionnaire circulated to students at the end of a financial accounting module (in July 2022). Satisfaction rates from students’ own perspectives were derived in terms of (i) overall satisfaction, (ii) learning outcomes, (iii) e-learning process, and (iv) pedagogical practices adopted. The assessment of satisfaction levels was determined through Likert-type items with responses ranging from a minimum score of 1 to the highest score of 5. Data gathered were subject to quantitative analysis: descriptive statistics, Pearson correlations, statistical tests, principal component analysis, and linear regression. High levels of satisfaction with distance education were uncovered. We found that pedagogical practices constitute the dimension that contributed the least (though, still importantly) to overall satisfaction as compared with learning outcomes and e-learning process. The results of this research offer the potential to contribute to the implementation of training offerings of online courses at other Portuguese HEIs as well as abroad.
Safety and Efficiency Evaluation Model for Converging Operation of Aircraft and Vehicles
Authors: Yang Kai , Yang Hongyu , Zhang Jianwei , Kang Rui
Published: April 2023

To explore the mixed traffic characteristics of vehicles and aircraft on the airport surface and solve the problem of real-time conflict detection at key intersections. According to the actual taxiing procedures and airport control rules in China, this paper focus on abstracting the mixed motion process of aircraft and vehicles in the maneuvering area, defining the convergent cross-safety operation scenario. To quantify the driver’s attention to safety separation and the degree of conservatism in adjusting speed, the vehicle deceleration rate and acceleration rate are defined with α as the exponent. Under the same spacing, the vehicle deceleration rate is directly proportional to α, and α is named the vehicle safety sensitivity. At the same time, the rules of speed change of vehicles and aircraft will be designed, and a convergent operation model of aircraft and vehicles will be proposed. Based on real-time speed and separation dynamic assessment of safety and efficiency under different traffic strategies, the computer simulation results show that vehicle safety sensitivity and deceleration rules determine the sequence of vehicles and aircraft when they are passing through in the short term and can affect the proportion of mixed traffic flow in the long run. The safety probability of vehicles passing the intersection first is negatively correlated with vehicle safety sensitivity, while the safety probability of aircraft passing the intersection first is correlated positively with vehicle safety sensitivity. The efficiency of passing without conflict with mixed traffic has an inverse relationship with vehicle safety sensitivity. When the vehicle safety sensitivity takes the value in the interval [0.4, 0.6], a mixed traffic flow with higher safety and efficiency, better stability, and a balanced ratio of locomotives can be obtained.
Identification of Lateral-Directional Aerodynamic Parameters for Aircraft Based on a Wind Tunnel Virtual Flight Test
Authors: Tai Shang , Wang Lixin , Wang Yanling , Lu Shiguang , Bu Chen , Yue Ting
Published: April 2023

In the early stages of aircraft design, a scaled model of the aircraft is installed in a wind tunnel for dynamic semi-free flight to approximate real flight, and the test data are then used to identify the aerodynamic parameters. However, the absence of the translational motion of the test model makes its flight dynamics different from those in free flight, and the effect of this difference on parameter identification needs to be investigated. To solve this problem, a 3-DOF wind tunnel virtual flight test device is built to fix the test model on a rotating mechanism, and the model is free to rotate in three axes through the deflection of the control surfaces. The flight dynamics equations for the wind tunnel virtual flight test are established and expressed as a decoupled form of the free flight force and the influence of the test support frame force on the model’s motions through linearization. The differences between wind tunnel virtual flight and free flight are analysed to develop a model for the identification of aerodynamic parameters. The selection of the lateral-directional excitation signal and the design method of its parameters are established based on the requirements for the identifiability of the aerodynamic derivatives, and a step-by-step method for the identification of aerodynamic force and moment derivatives is established. The aerodynamic parameter identification results of a blended wing body aircraft show that the identification method proposed in this paper can obtain results with high accuracy, and the response of the modified motion model is consistent with that of the free flight motion model.
Effects of the In ovo Administration of the 6/85 Mycoplasma gallisepticum Vaccine on Layer Chicken Embryo Hatchability and Early Posthatch Performance
Authors: Alqhtani Abdulmohsen H. , Fatemi Seyed Abolghasem , Elliott Katie Elaine Collins , Branton Scott L. , Evans Jeff Dwayne , Peebles Edgar David
Published: April 2023

In ovo administration as a possible alternative method of 6/85 MG vaccination was assessed. After 18 days of incubation (doi), the eggs were administered a particular dosage of a live attenuated 6/85 MG vaccine in either the air cell (AC) or amnion (AM). The treatments included non-injected eggs and eggs injected into the AC or AM with diluent alone as controls. Treatments also included eggs injected with diluent, which contained 1.73 × 102, or 1.73 × 104 CFU of 6/85 MG. Hatchability of viable injected eggs (HI) and residual embryonic mortality were determined at 22 doi. At hatch and at three weeks posthatch, one hatched chick per treatment replicate was bled and swabbed for the detection of 6/85 MG in the choanal cleft using PCR, serum plate agglutination (SPA), and ELISA methods. The results show that AC in ovo injection of 6/85 MG had no negative impacts on HI or on the live performance of pullets, but that it failed to provide adequate protection (p ≤ 0.0001) in hatchlings or three-week-old pullets. The 1.73 × 104 6/85 MG CFU dosage injected into the AM decreased the hatchability of injected eggs containing viable embryos (HI; p = 0.009) and was associated with a significant increase in late dead mortality (p = 0.001). Hatchling and three-week-old chick mortalities (p = 0.008) were significantly greater in the 1.73 × 104 CFU-AM treatment group in comparison with the other treatment groups. In addition, the 1.73 and 1.73 × 102 6/85 MG-AM treatments had no negative effects on the hatching process or on posthatch growth, and the 1.73 × 102 6/85 MG-AM treatment was more effective in the protection of pullets against MG (p ≤ 0.0001) as compared with the low dosage and non-injected treatment groups. Further research is needed to examine the influence of the 6/85 MG in ovo vaccine on layer immune competence.
Investigation of the Expression Pattern and Functional Role of miR-10b in Intestinal Inflammation
Authors: Wu Zijuan , Pi Guolin , Song Wenxin , Li Yali
Published: April 2023

Implications of miRNAs for animal health management in livestock remain elusive. To identify suitable miRNAs as monitoring biomarkers, piglets were randomly selected for sampling on days 0, 1, 3, 7, and 14 post-weaning. The results show that miR-10b levels in the villus upper cells of the jejunum on days 3 and 7 were significantly lower than that on day 14 post-weaning and reduced by approximately 30% on day 3 and 55% on day 7 compared to day 0. In contrast, miR-10b in crypt cells decreased by approximately 82% on day 7 and 64% on day 14 compared with day 0. Next, miR-10 knockout mice and wild-type mice were subjected to dextran sulfate sodium (DSS) for 7 days. The findings demonstrate that mice lacking miR-10b were more susceptible to DSS administration, as demonstrated by worse survival, greater weight loss, more severe tissue damage, and increased intestinal permeability. Moreover, the increased disease severity was correlated with enhanced macrophage infiltration, coincident with significantly elevated pro-inflammatory mediators and immunoglobulins. Bioinformatic analysis further reveals that the enriched pathways were mainly involved in host immune responses, and Igtp was identified as a potential target of miR-10b. These findings may provide new strategies for future interventions for swine health and production.
Rawls and Animal Moral Personality
Authors: Baldwin Guy
Published: April 2023

The relationship between animal rights and contractarian theories of justice such as that of Rawls has long been vexed. In this article, I contribute to the debate over the possibility of inclusion of animals in Rawls’s theory of justice by critiquing the rationale he gives for their omission: that they do not possess moral personality. Contrary to Rawls’s assumptions, it appears that some animals may possess the moral powers that comprise moral personality, albeit to a lesser extent than most humans. Some animals can act in pursuit of preferences and desires (and communicate them non-verbally), which might be taken as implicitly selecting a conception of the good; further, scientific research demonstrating inequity aversion and social play behaviors suggests that some animals can have a sense of justice relating to their own social groups. I conclude that Rawls’s theory needs to acknowledge any animals that can be considered to meet the threshold of moral personality, while the concept of moral personality as a range property may also require reconsideration.
Gluten-Free Bread Enriched with Artichoke Leaf Extract In Vitro Exerted Antioxidant and Anti-Inflammatory Properties
Authors: Vacca Mirco , Pinto Daniela , Annunziato Alessandro , Ressa Arianna , Calasso Maria , Pontonio Erica , Celano Giuseppe , De Angelis Maria
Published: April 2023

Due to its high nutritional value and broad beneficial effects, the artichoke plant (Cynara cardunculus L.) is an excellent healthy food candidate. Additionally, the artichoke by-products are usually discarded even though they still contain a huge concentration of dietary fibers, phenolic acids, and other micronutrients. The present work aimed to characterize a laboratory-made gluten-free bread (B) using rice flour supplemented with a powdered extract from artichoke leaves (AEs). The AE, accounting for the 5% of titratable chlorogenic acid, was added to the experimental gluten-free bread. Accounting for different combinations, four different bread batches were prepared. To evaluate the differences, a gluten-free type-II sourdough (tII-SD) was added in two doughs (SB and SB-AE), while the related controls (YB and YB-AE) did not contain the tII-SD. Profiling the digested bread samples, SB showed the lowest glycemic index, while SB-AE showed the highest antioxidant properties. The digested samples were also fermented in fecal batches containing viable cells from fecal microbiota samples obtained from healthy donors. Based on plate counts, no clear tendencies emerged concerning the analyzed microbial patterns; by contrast, when profiling volatile organic compounds, significant differences were observed in SB-AE, exhibiting the highest scores of hydrocinnamic and cyclohexanecarboxylic acids. The fecal fermented supernatants were recovered and assayed for healthy properties on human keratinocyte cell lines against oxidative stress and for effectiveness in modulating the expression of proinflammatory cytokines in Caco-2 cells. While the first assay emphasized the contribution of AE to protect against stressor agents, the latter enlightened how the combination of SB with AE decreased the cellular TNF-α and IL1-β expression. In conclusion, this preliminary study suggests that the combination of AE with sourdough biotechnology could be a promising tool to increase the nutritional and healthy features of gluten-free bread.
Analysis of Single- and Double-Stranded DNA Damage in Osteoblastic Cells after Hyperbaric Oxygen Exposure
Authors: Schönrock Nele , Tillmans Frauke , Sebens Susanne , Kähler Wataru , Klapa Sebastian , Rieger Bente , Scherthan Harry , Koch Andreas
Published: April 2023

(1) Background: Hyperbaric oxygen (HBO) exposure induces oxidative stress that may lead to DNA damage, which has been observed in human peripheral blood lymphocytes or non-human cells. Here, we investigated the impact of hyperbaric conditions on two human osteoblastic cell lines: primary human osteoblasts, HOBs, and the osteogenic tumor cell line SAOS-2. (2) Methods: Cells were exposed to HBO in an experimental hyperbaric chamber (4 ATA, 100% oxygen, 37 °C, and 4 h) or sham-exposed (1 ATA, air, 37 °C, and 4 h). DNA damage was examined before, directly after, and 24 h after exposure with an alkaline comet assay and detection of γH2AX+53BP1 colocalizing double-strand break (DSB) foci and apoptosis. The gene expression of TGFß-1, HO-1, and NQO1, involved in antioxidative functions, was measured with qRT-PCR. (3) Results: The alkaline comet assay showed significantly elevated levels of DNA damage in both cell lines after 4 h of HBO, while the DSB foci were similar to sham. γH2AX analysis indicated a slight increase in apoptosis in both cell lines. The increased expression of HO-1 in HOB and SAOS-2 directly after exposure suggested the induction of an antioxidative response in these cells. Additionally, the expression of TGF-ß1 was negatively affected in HOB cells 4 h after exposure. (4) Conclusions: in summary, this study indicates that osteoblastic cells are sensitive to the DNA-damaging effects of hyperbaric hyperoxia, with the HBO-induced DNA damage consisting largely of single-strand DNA breaks that are rapidly repaired.