bluuebunny/crossref_metadata_2025 huggingface dataset:
Cervical Cancer Classification From Pap Smear Images Using Deep Convolutional Neural Network Models
Authors: Tan Sher Lyn , Selvachandran Ganeshsree , Ding Weiping , Paramesran Raveendran , Kotecha Ketan
Published: November 2023

As one of the most common female cancers, cervical cancer often develops years after a prolonged and reversible pre-cancerous stage. Traditional classification algorithms used for detection of cervical cancer often require cell segmentation and feature extraction techniques, while convolutional neural network (CNN) models demand a large dataset to mitigate over-fitting and poor generalization problems. To this end, this study aims to develop deep learning models for automated cervical cancer detection that do not rely on segmentation methods or custom features. Due to limited data availability, transfer learning was employed with pre-trained CNN models to directly operate on Pap smear images for a seven-class classification task. Thorough evaluation and comparison of 13 pre-trained deep CNN models were performed using the publicly available Herlev dataset and the Keras package in Google Collaboratory. In terms of accuracy and performance, DenseNet-201 is the best-performing model. The pre-trained CNN models studied in this paper produced good experimental results and required little computing time.

Graphical Abstract
Formulation, pharmacokinetics, and antibacterial activity of florfenicol-loaded niosome
Authors: Abonashey Shimaa G. , Hassan Hatem A. F. M. , Shalaby Mostafa A. , Fouad Amr Gamal , Mobarez Elham , El-Banna Hossny A.
Published: November 2023

The growing interest in employing nano-sized pharmaceutical formulations in veterinary medicine has prompted the exploration of the novel nanocarriers’ ability to augment the therapeutic outcome. In this study, we harnessed niosomes, spherical nanocarriers formed through non-ionic surfactant self-assembly, to enhance the therapeutic efficacy of the broad-spectrum antibiotic florfenicol. Pre-formulation studies were conducted to identify the optimal parameters for preparing florfenicol-loaded niosomes (FLNs). These studies revealed that the formulation that consisted of Span 60, cholesterol, and dihexadecyl phosphate (DDP) at a molar ratio of 1:1:0.1 exhibited the highest entrapment efficiency (%EE) and uniform size distribution. In vitro antibacterial testing demonstrated the niosomal capacity to significantly reduce florfenicol minimum inhibitory concentration (MIC) against E. coli and S. aureus. Pharmacokinetic profiles of free florfenicol and FLN were assessed following oral administration of 30 mg florfenicol/kg body weight to healthy or E. coli–infected chickens. FLN exhibited a substantially higher maximum plasma concentration (Cmax) of florfenicol compared to free florfenicol. Furthermore, FLN showed significantly higher area under the curve (AUC0–t) than free florfenicol as revealed from the relative bioavailability studies. Lethal dose (LD) 50 values for both free florfenicol and FLN exceeded 5 g/kg of body weight, indicating high safety profile. Assessment of mortality protection in mice against lethal E. coli infections showed the significantly higher capability of FLN to improve the survival rate (75%) than free florfenicol (25%). Collectively, these findings demonstrate the niosomal ability to improve the oral bioavailability as well as the antibacterial activity of the incorporated veterinary antibiotic florfenicol.

Graphical abstract
Longevity trend in Germany
Authors: Reitzner Matthias
Published: November 2023

In Germany, a trend for decreasing mortality probabilities has been observed in the last 50 years, yielding an increasing life expectancy. The German Actuarial Association DAV offers a standard method for modeling this longevity trend in calculations concerning life insurance by using the life table DAV 2004R. In this note it is investigated, whether or to which extent the longevity function of the DAV 2004R can be used for calculating the expected total number of deaths in Germany.
Strategies for addressing conflicts arising from blue growth initiatives: insights from three case studies in South Africa
Authors: Sowman Merle , Mbatha Philile , von Holdt Johanna
Published: November 2023

South Africa has vigorously embraced the concept of the ‘blue economy’ and is aggressively pursuing a blue growth strategy to expand the ocean economy, create jobs, and alleviate poverty. However, many of these ‘blue initiatives’ are leading to conflicts amongst various stakeholders with different histories, relationships with resources and areas, worldviews, and values. Investment in the ocean economy is being prioritized by government and planning, environmental assessment, and decision-making processes are being fast-tracked. Consequently, historical inequities as well as environmental and social justice considerations are not being given due consideration. Communities are not being effectively consulted. This has resulted in tensions and conflicts amongst proponents of these projects and local communities living in areas affected by these initiatives. We examine the drivers of conflict and then explore the strategies that local communities and their social partners have employed in these case studies to challenge contentious developments, defend coastal and marine areas, and make their voices heard. The cases involve conflicts over air quality in an expanding marine industrial zone at Saldanha Bay, prospecting and mining applications in the vicinity of the Olifants Estuary in the Western Cape, and the expansion of the Richard’s Bay Port, mining activities, and conservation initiatives in KwaZulu-Natal. The barriers and potential opportunities to opening up deliberative spaces, shifting values and views, and co-producing knowledge, in contexts that are characterised by structural inequality, poverty, and power asymmetries, are discussed.
Molecular Autism Research in Africa: Emerging Themes and Prevailing Disparities
Authors: Frickel Emma , Mahony Caitlyn , Bam Sophia , Buchanan Erin , van der Watt Mignon , O’Ryan Colleen
Published: November 2023

African populations are consistently underrepresented in molecular research on autism spectrum disorders (ASD). Yet, Africa’s genetic diversity could reveal novel mechanisms associated with ASD etiology. We review the molecular ASD research from Africa between 2016–2022, highlighting region-specific limitations, opportunities, and areas of progress. We emphasize a need to advance null-hypothesis based molecular studies in Africa, particularly in critically understudied Sub-Saharan African (SSA) populations. Using South Africa as a case study, we show that this geographical disparity is not solely attributable to sociocultural barriers nor to an absence of molecular research infrastructure. We emphasize the importance of interdisciplinary collaboration within SSA and internationally to harness existing infrastructure for the expansion of molecular ASD research in Africa.
An orthogeriatric service can reduce prolonged hospital length of stay in hospital for older adults admitted with hip fractures: a monocentric study
Authors: Lisk Radcliffe , Yeong Keefai , Fluck David , Robin Jonathan , Fry Christopher Henry , Han Thang Sieu
Published: November 2023

Background

The Blue Book (2005), recommended guidelines for patients care with fragility fractures. Together with introduction of a National Hip Fracture Database Audit and Best Practice Tariff model to financially incentivise hospitals by payment of a supplement for patients whose care satisfied six clinical standards), have improved hip fracture after-care. However, there is a lack of data-driven evidence to support its effectiveness. We aimed to verify the impact of an orthogeriatric service on hospital length of stay (LOS)—duration from admission to discharge.

Methods

We conducted a repeated cross-sectional study over a 10 year period of older individuals aged ≥ 60 years admitted with hip fractures to a hospital.

Results

Altogether 2798 patients, 741 men and 2057 women (respective mean ages; 80.5 ± 10.6 and 83.2 ± 8.9 years) were admitted from their own homes with a hip fracture and survived to discharge. Compared to 2009–2014, LOS during 2015–2019, when the orthogeriatric service was fully implemented, was shorter for all discharge destinations: 10.4 vs 17.5 days (P < 0.001). Each discharge destination showed reductions: back to own homes, 9.7 vs 17.7 days (P < 0.001); to rehabilitation units: 10.8 vs 13.1 days (P < 0.001); to residential care: 15.4 vs 26.2 days (P = 0.001); or nursing care, 24.4 vs 53.1 days (P < 0.001). During 2009–2014, the risk of staying > 3 weeks in hospital was greater by six-fold and pressure ulcers by three-fold. The number of bed days for every thousand patients per year was also shortened during 2015–2019 by: 1665 days for discharge back to own homes; 469 days with transfer to rehabilitation units; 1258 days for discharge to residential care, and 5465 days to nursing care. Estimated annual savings (2017 costs) per thousand patients after complete establishment of the service was about £2.7 m.

Conclusions

Implementation of an orthogeriatric service generated significant reductions in hospital LOS for all patients, with associated cost-savings, especially for those discharged to nursing care.
Understanding Sociodemographic Factors among Hispanics Through a Population-Based Study on Testicular Cancer in Mexico
Authors: Ríos-Rodríguez Juan Alberto , Montalvo-Casimiro Michel , Álvarez-López Diego Ivar , Reynoso-Noverón Nancy , Cuevas-Estrada Berenice , Mendoza-Pérez Julia , Jiménez-Ríos Miguel A. , Wegman-Ostrosky Talia , Salcedo-Tello Pamela , Scavuzzo Anna , Castro-Hernández Clementina , Herrera Luis A. , González-Barrios Rodrigo
Published: November 2023

Testicular cancer (TCa) is a rare malignancy affecting young men worldwide. Sociodemographic factors, especially socioeconomic level (SEL) and healthcare access, seem to impact TCa incidence and outcomes, particularly among Hispanic populations. However, limited research has explored these variables in Hispanic groups. This study aimed to investigate sociodemographic and clinical factors in Mexico and their role in health disparities among Hispanic TCa patients. We retrospectively analyzed 244 Mexican TCa cases between 2007 and 2020 of a representative cohort with diverse social backgrounds from a national reference cancer center. Logistic regression identified risk factors for fatality: non-seminoma histology, advanced stage, and lower education levels. Age showed a significant trend as a risk factor. Patient delay and healthcare distance lacked significant associations. Inadequate treatment response and chemotherapy resistance were more likely in advanced stages, while higher education positively impacted treatment response. Cox regression highlighted non-seminoma histology, below-median SEL, higher education, and advanced-stage survival rates. Survival disparities emerged based on tumor histology and patient SEL. This research underscores the importance of comprehensive approaches that integrate sociodemographic, biological, and environmental factors to address health disparities improving outcomes through personalized interventions in Hispanic individuals with TCa.
Bridging the evidence gap: A review and research protocol for outdoor mental health therapies for young Australians
Authors: Flies Emily J. , Pryor Anita , Henderson-Wilson Claire , Turner Megan , Roydhouse Jessica , Patrick Rebecca , O’Shea Melissa , Norris Kimberly , Martin Angela , Marsh Pauline , Bartlett Larissa , Azghadi Mostafa Rahimi , Neil Amanda
Published: November 2023

Internationally, over 60% of all lifetime cases of mental health disorders are identified as emerging by 25 years of age. In Australia, young people (aged 16–24 years) report the highest prevalence of mental health problems. Acceptability of mainstream services for young people is a concern, particularly for clients 18–25 years, heterosexual males and certain marginalised communities. With unaddressed distress in young people a precursor to poor, potentially lifelong mental ill-health trajectories, the provision of acceptable, and accessible mental health services remains a critical system imperative.

Outdoor therapies, such as outdoor talking therapies, present an option for increasing the breadth of mental health interventions available to young people. Reported benefits of outdoor therapies include improved self-esteem and confidence, positive and negative affect, stress reduction and restoration, social benefits, and resilience. As outdoor therapies draw on multidisciplinary skillsets, this modality has the potential to expand services beyond existing workforce capacities. However, there are evidence gaps that must be addressed before mainstreaming of this treatment modality can occur.

Here we overview the existing evidence base for outdoor talking therapies, as a form of outdoor mental healthcare, to determine their appropriateness as an effective and efficient treatment modality for young people with psychological distress in Australia and elsewhere. We then propose a research protocol designed to determine the acceptability, efficacy and efficiency of ‘outdoor talking therapies’. Our aim is to help address identified youth mental healthcare service shortages in Australia, and potentially support the health of our mental healthcare workforce.
Literatur und Psychologie. Zum Erkenntnispotenzial fiktionaler Welten
Authors: Sichler Ralph
Published: November 2023

Die Überlegungen in diesem Beitrag gehen der Frage nach, ob und inwieweit Literatur, also eine Gattung der Kunst, als Quelle der Erfahrung zu Erkenntnissen der Psychologie beitragen kann. Ausgehend von der Zurückhaltung der akademischen Psychologie gegenüber den Werken der Dichtung und ihren möglichen Beitrag zur psychologischen Erkenntnisbildung wird auf der Grundlage der Philosophie der symbolischen Formen von Ernst Cassirer argumentiert, dass aufgrund der jeweils gegebenen sprachlichen Verfasstheit die fiktionale Welt der Literatur in gleicher Weise präsent ist wie die faktische Alltagswelt. Mithilfe des von Robert Musil eingeführten Möglichkeitssinns wird allerdings auf die Besonderheit der durch Literatur erzeugten Welten aufmerksam gemacht. Es wird deutlich, dass Dichtung nicht nur eine wichtige Quelle psychologischer Erfahrungsbildung darstellt, sondern ebenso wesentlich zur Möglichkeit der Reflexion von Lebensumständen und Lebensformen in der Gesellschaft beiträgt. Auf diese Weise verfolgt die Psychologie nicht nur ein Erkenntnisinteresse, sondern auch einen Bildungsauftrag.
What do patients want from therapy? Understanding treatment goals of patients with long-term conditions referred for cognitive behavioural therapy in primary care
Authors: Sawrikar Vilas , Newton Christopher , Nicklas Leeanne
Published: November 2023

Integrating cognitive behavioural therapy (CBT) into primary care for patients with long-terms conditions (LTCs) is a priority for the National Health Service (NHS) in the United Kingdom (UK). To inform delivery of cognitive behavioural interventions for this clinical population, the aim of this study was to evaluate the major treatment goal themes of patients with LTCs. A single group mixed-methods design was used to analys treatment goals and their association with patient characteristics. A total of n=222 patients (86 males; 132 females) who participated in a service development evaluation of the Accessible Depression and Anxiety Psychological Therapies for Individuals with Long-Term Conditions in Scotland (UK) were selected for inclusion if they reported at least one treatment goal at assessment. Data were drawn from routine outcome measures that recorded information in relation to client demographics, physical conditions, mental health, functioning and treatment goals. Participants freely reported up to three goals as part of assessment. Thematic analysis identified four major goal themes ranked in the following order of frequency: functioning, emotional health, condition management, and self-appraisal. Wanting to improve functioning was positively associated with age and depression, and negatively associated with anxiety. No other patient characteristics were associated with any of the major themes. Patients with LTCs referred to CBT in primary care can have wide-ranging goals that only partially correspond with their mental health status. Practitioners and service providers need to flexibly deliver CBT to enhance the individual relevance of therapy which is tailored to patient’s goals.

Key learning aims

(1)

Treatment goals are fundamental to a better understanding of how best to assess and plan treatments that meet the needs of patients with long-term conditions.

(2)

We highlight the need to enhance practitioner competencies in aligning treatment with patient’s goals to ensure goal-based decision-making is achieved in practice.

(3)

Key areas of goal-oriented therapy for patients with long-term conditions include integrating aspects of wanting to improve functioning, emotional health, condition management, and self-appraisal. These aspects should represent primary outcomes of treatment.
Diagnosis and management of primary hyperaldosteronism in patients with hypertension: a practical approach endorsed by the British and Irish Hypertension Society
Authors: Faconti Luca , Kulkarni Spoorthy , Delles Christian , Kapil Vikas , Lewis Philip , Glover Mark , MacDonald Thomas M. , Wilkinson Ian B.
Published: November 2023

Alongside the lack of homogeneity among international guidelines and consensus documents on primary hyperaldosteronism, the National UK guidelines on hypertension do not provide extensive recommendations regarding the diagnosis and management of this condition. Local guidelines vary from area to area, and this is reflected in the current clinical practice in the UK. In an attempt to provide support to the clinicians involved in the screening of subjects with hypertension and clinical management of suspected cases of primary hyperaldosteronism the following document has been prepared on the behalf of the BIHS Guidelines and Information Service Standing Committee. Through remote video conferences, the authors of this document reviewed an initial draft which was then circulated among the BIHS Executive members for feedback. A survey among members of the BIHS was carried out in 2022 to assess screening strategies and clinical management of primary hyperaldosteronism in the different regions of the UK. Feedback and results of the survey were then discussed and incorporated in the final document which was approved by the panel after consensus was achieved considering critical review of existing literature and expert opinions. Grading of recommendations was not performed in light of the limited available data from properly designed randomized controlled trials.
Recent advances and research progress on microsystems and bioeffects of terahertz neuromodulation
Authors: Liu Meiting , Liu Juntao , Liang Wei , Lu Botao , Fan Penghui , Song Yilin , Wang Mixia , Wu Yirong , Cai Xinxia
Published: November 2023

Terahertz waves can interact with the nervous system of organisms under certain conditions. Compared to common optical modulation methods, terahertz waves have the advantages of low photon energy and low risk; therefore, the use of terahertz waves to regulate the nervous system is a promising new method of neuromodulation. However, most of the research has focused on the use of terahertz technology for biodetection, while relatively little research has been carried out on the biological effects of terahertz radiation on the nervous system, and there are almost no review papers on this topic. In the present article, we begin by reviewing principles and objects of research regarding the biological effects of terahertz radiation and summarizing the current state of related research from a variety of aspects, including the bioeffects of terahertz radiation on neurons in vivo and in vitro, novel regulation and detection methods with terahertz radiation devices and neural microelectrode arrays, and theoretical simulations of neural information encoding and decoding. In addition, we discuss the main problems and their possible causes and give some recommendations on possible future breakthroughs. This paper will provide insight and assistance to researchers in the fields of neuroscience, terahertz technology and biomedicine.
The EZH2-H3K27me3 axis modulates aberrant transcription and apoptosis in cyclophosphamide-induced ovarian granulosa cell injury
Authors: Chen Yingyan , Ai Leilei , Zhang Yingyi , Li Xiang , Xu Shiqian , Yang Weijie , Jin Jiamin , Ma Yerong , Hu Zhanhong , Zhang Yinli , Rong Yan , Zhang Songying
Published: November 2023

Chemotherapy-induced ovarian damage and infertility are significant concerns for women of childbearing age with cancer; however, the underlying mechanisms are still not fully understood. Our study has revealed a close association between epigenetic regulation and cyclophosphamide (CTX)-induced ovarian damage. Specifically, CTX and its active metabolite 4-hydroperoxy cyclophosphamide (4-HC) were found to increase the apoptosis of granulosa cells (GCs) by reducing EZH2 and H3K27me3 levels, both in vivo and in vitro. Furthermore, RNA-seq and CUT&Tag analyses revealed that the loss of H3K27me3 peaks on promoters led to the overactivation of genes associated with transcriptional regulation and apoptosis, indicating that stable H3K27me3 status could help to provide a safeguard against CTX-induced ovarian damage. Administration of the H3K27me3-demethylase inhibitor, GSK-J4, prior to CTX treatment could partially mitigate GC apoptosis by reversing the reduction of H3K27me3 and the aberrant upregulation of specific genes involved in transcriptional regulation and apoptosis. GSK-J4 could thus potentially be a protective agent for female fertility when undergoing chemotherapy. The results provide new insights into the mechanisms for chemotherapy injury and future clinical interventions for fertility preservation.
Hofbauer cell function in the term placenta associates with adult cardiovascular and depressive outcomes
Authors: Fitzgerald Eamon , Shen Mojun , Yong Hannah Ee Juen , Wang Zihan , Pokhvisneva Irina , Patel Sachin , O’Toole Nicholas , Chan Shiao-Yng , Chong Yap Seng , Chen Helen , Gluckman Peter D. , Chan Jerry , Lee Patrick Kia Ming , Meaney Michael J.
Published: November 2023

Pathological placental inflammation increases the risk for several adult disorders, but these mediators are also expressed under homeostatic conditions, where their contribution to adult health outcomes is unknown. Here we define an inflammation-related expression signature, primarily expressed in Hofbauer cells of the term placenta and use expression quantitative trait loci to create a polygenic score (PGS) predictive of its expression. Using this PGS in the UK Biobank we conduct a phenome-wide association study, followed by Mendelian randomization and identify protective, sex-dependent effects of the placental module on cardiovascular and depressive outcomes. Genes differentially regulated by intra-amniotic infection and preterm birth are over-represented within the module. We also identify aspirin as a putative modulator of this inflammation-related signature. Our data support a model where disruption of placental Hofbauer cell function, due to preterm birth or prenatal infection, contributes to the increased risk of depression and cardiovascular disease observed in these individuals.
Bacteria can maintain rRNA operons solely on plasmids for hundreds of millions of years
Authors: Anda Mizue , Yamanouchi Shun , Cosentino Salvatore , Sakamoto Mitsuo , Ohkuma Moriya , Takashima Masako , Toyoda Atsushi , Iwasaki Wataru
Published: November 2023

It is generally assumed that all bacteria must have at least one rRNA operon (rrn operon) on the chromosome, but some strains of the genera Aureimonas and Oecophyllibacter carry their sole rrn operon on a plasmid. However, other related strains and species have chromosomal rrn loci, suggesting that the exclusive presence of rrn operons on a plasmid is rare and unlikely to be stably maintained over long evolutionary periods. Here, we report the results of a systematic search for additional bacteria without chromosomal rrn operons. We find that at least four bacterial clades in the phyla Bacteroidota, Spirochaetota, and Pseudomonadota (Proteobacteria) lost chromosomal rrn operons independently. Remarkably, Persicobacteraceae have apparently maintained this peculiar genome organization for hundreds of millions of years. In our study, all the rrn-carrying plasmids in bacteria lacking chromosomal rrn loci possess replication initiator genes of the Rep_3 family. Furthermore, the lack of chromosomal rrn operons is associated with differences in copy numbers of rrn operons, plasmids, and chromosomal tRNA genes. Thus, our findings indicate that the absence of rrn loci in bacterial chromosomes can be stably maintained over long evolutionary periods.
Single amino acid change alters specificity of the multi-allelic wheat stem rust resistance locus SR9
Authors: Zhang Jianping , Nirmala Jayaveeramuthu , Chen Shisheng , Jost Matthias , Steuernagel Burkhard , Karafiatova Mirka , Hewitt Tim , Li Hongna , Edae Erena , Sharma Keshav , Hoxha Sami , Bhatt Dhara , Antoniou-Kourounioti Rea , Dodds Peter , Wulff Brande B. H. , Dolezel Jaroslav , Ayliffe Michael , Hiebert Colin , McIntosh Robert , Dubcovsky Jorge , Zhang Peng , Rouse Matthew N. , Lagudah Evans
Published: November 2023

Most rust resistance genes thus far isolated from wheat have a very limited number of functional alleles. Here, we report the isolation of most of the alleles at wheat stem rust resistance gene locus SR9. The seven previously reported resistance alleles (Sr9a, Sr9b, Sr9d, Sr9e, Sr9f, Sr9g, and Sr9h) are characterised using a synergistic strategy. Loss-of-function mutants and/or transgenic complementation are used to confirm Sr9b, two haplotypes of Sr9e (Sr9e_h1 and Sr9e_h2), Sr9g, and Sr9h. Each allele encodes a highly related nucleotide-binding site leucine-rich repeat (NB-LRR) type immune receptor, containing an unusual long LRR domain, that confers resistance to a unique spectrum of isolates of the wheat stem rust pathogen. The only SR9 protein effective against stem rust pathogen race TTKSK (Ug99), SR9H, differs from SR9B by a single amino acid. SR9B and SR9G resistance proteins are also distinguished by only a single amino acid. The SR9 allelic series found in the B subgenome are orthologs of wheat stem rust resistance gene Sr21 located in the A subgenome with around 85% identity in protein sequences. Together, our results show that functional diversification of allelic variants at the SR9 locus involves single and multiple amino acid changes that recognize isolates of wheat stem rust.
Deciphering a critical role of uterine epithelial SHP2 in parturition initiation at single cell resolution
Authors: Liu Meng , Ji Mengjun , Cheng Jianghong , Li Yingzhe , Tian Yingpu , Zhao Hui , Wang Yang , Zhu Sijing , Zhang Leilei , Xu Xinmei , Feng Gen-Sheng , Liang Xiaohuan , Bao Haili , Tang Yedong , Kong Shuangbo , Lu Jinhua , Wang Haibin , Lu Zhongxian , Deng Wenbo
Published: November 2023

The timely onset of female parturition is a critical determinant for pregnancy success. The highly heterogenous maternal decidua has been increasingly recognized as a vital factor in setting the timing of labor. Despite the cell type specific roles in parturition, the role of the uterine epithelium in the decidua remains poorly understood. This study uncovers the critical role of epithelial SHP2 in parturition initiation via COX1 and COX2 derived PGF2α leveraging epithelial specificShp2knockout mice, whose disruption contributes to delayed parturition initiation, dystocia and fetal deaths. Additionally, we also show that there are distinct types of epithelium in the decidua approaching parturition at single cell resolution accompanied with profound epithelium reformation via proliferation. Meanwhile, the epithelium maintains the microenvironment by communicating with stromal cells and macrophages. The epithelial microenvironment is maintained by a close interaction among epithelial, stromal and macrophage cells of uterine stromal cells. In brief, this study provides a previously unappreciated role of the epithelium in parturition preparation and sheds lights on the prevention of preterm birth.
Detangling electrolyte chemical dynamics in lithium sulfur batteries by operando monitoring with optical resonance combs
Authors: Liu Fu , Lu Wenqing , Huang Jiaqiang , Pimenta Vanessa , Boles Steven , Demir-Cakan Rezan , Tarascon Jean-Marie
Published: November 2023

Challenges in enabling next-generation rechargeable batteries with lower cost, higher energy density, and longer cycling life stem not only from combining appropriate materials, but from optimally using cell components. One-size-fits-all approaches to operational cycling and monitoring are limited in improving sustainability if they cannot utilize and capture essential chemical dynamics and states of electrodes and electrolytes. Herein we describe and show how the use of tilted fiber Bragg grating (TFBG) sensors to track, via the monitoring of both temperature and refractive index metrics, electrolyte-electrode coupled changes that fundamentally control lithium sulfur batteries. Through quantitative sensing of the sulfur concentration in the electrolyte, we demonstrate that the nucleation pathway and crystallization of Li2S and sulfur govern the cycling performance. With this technique, a critical milestone is achieved, not only towards developing chemistry-wise cells (in terms of smart battery sensing leading to improved safety and health diagnostics), but further towards demonstrating that the coupling of sensing and cycling can revitalize known cell chemistries and break open new directions for their development.
Fungal secondary metabolism is governed by an RNA-binding protein CsdA/RsdA complex
Authors: Song Zili , Zhou Shuang , Zhang Hongjiao , Keller Nancy P. , Oakley Berl R. , Liu Xiao , Yin Wen-Bing
Published: November 2023

Production of secondary metabolites is controlled by a complicated regulatory network in eukaryotic cells. Several layers of regulators are involved in this process, ranging from pathway-specific regulation, to epigenetic control, to global regulation. Here, we discover that interaction of an RNA-binding protein CsdA with a regulator RsdA coordinates fungal secondary metabolism. Employing a genetic deletion approach and transcriptome analysis as well as metabolomics analysis, we reveal that CsdA and RsdA synergistically regulate fungal secondary metabolism comprehensively. Mechanistically, comprehensive genetic and biochemical studies prove that RsdA and CsdA co-localize in the nucleus and physically interact to achieve their functions. In particular, we demonstrate that CsdA mediates rsdA expression by binding specific motif “GUCGGUAU” of its pre-mRNA at a post-transcriptional level. We thus uncover a mechanism in which RNA-binding protein physically interacts with, and controls the expression level of, the RsdA to coordinate fungal secondary metabolism.
Quasiperiodic circuit quantum electrodynamics
Authors: Herrig T. , Pixley J. H. , König E. J. , Riwar R.-P.
Published: November 2023

Superconducting circuits are an extremely versatile platform to realize quantum information hardware and to emulate topological materials. We here show how a simple arrangement of capacitors and conventional superconductor-insulator-superconductor junctions can realize an even broader class of systems, in the form of a nonlinear capacitive element which is quasiperiodic with respect to the quantized Cooper-pair charge. Our setup allows to create protected Dirac points defined in the transport degrees of freedom, whose presence leads to a suppression of the classical finite-frequency current noise. Furthermore, the quasiperiodicity can emulate Anderson localization in charge space, measurable via vanishing charge quantum fluctuations. The realization by means of the macroscopic transport degrees of freedom allows for a straightforward generalization to arbitrary dimensions and implements truly non-interacting versions of the considered models. As an outlook, we discuss potential ideas to simulate a transport version of the magic-angle effect known from twisted bilayer graphene.
A Large Ensemble Global Dataset for Climate Impact Assessments
Authors: Gao Xiang , Sokolov Andrei , Schlosser C. Adam
Published: November 2023

We present a self-consistent, large ensemble, high-resolution global dataset of long‐term future climate, which accounts for the uncertainty in climate system response to anthropogenic emissions of greenhouse gases and in geographical patterns of climate change. The dataset is developed by applying an integrated spatial disaggregation (SD) − bias-correction (BC) method to climate projections from the MIT Integrated Global System Model (IGSM). Four emission scenarios are considered that represent energy and environmental policies and commitments of potential future pathways, namely, Reference, Paris Forever, Paris 2 °C and Paris 1.5 °C. The dataset contains nine key meteorological variables on a monthly scale from 2021 to 2100 at a spatial resolution of 0.5°x 0.5°, including precipitation, air temperature (mean, minimum and maximum), near-surface wind speed, shortwave and longwave radiation, specific humidity, and relative humidity. We demonstrate the dataset’s ability to represent climate-change responses across various regions of the globe. This dataset can be used to support regional-scale climate-related impact assessments of risk across different applications that include hydropower, water resources, ecosystem, agriculture, and sustainable development.
Image facilitated assessment of intra-spike variation in grain size in wheat under high temperature and drought stress
Authors: Thakur Vidisha , Rane Jagadish , Pandey Girish Chandra , Yadav Satish
Published: November 2023

In wheat (Triticum aestivum L.), the grain size varies according to position within the spike. Exposure to drought and high temperature stress during grain development in wheat reduces grain size, and this reduction also varies across the length of the spike. We developed the phenomics approach involving image-based tools to assess the intra-spike variation in grain size. The grains were arranged corresponding to the spikelet position and the camera of smart phone was used to acquire 333 images. The open-source software ImageJ was used to analyze features of each grain and the image-derived parameters were used to calculate intra-spike variation as standard deviation (ISVAD). The effect of genotype and environment were highly significant on the ISVAD of grain area. Sunstar and Raj 4079 contrasted in the ISVAD of grain area under late sown environment, and RNA sequencing of the spike was done at 25 days after anthesis. The genes for carbohydrate transport and stress response were upregulated in Sunstar as compared to Raj 4079, suggesting that these play a role in intra-spike assimilate distribution. The phenomics method developed may be useful for grain phenotyping and identifying germplasm with low intra-spike variation in grain size for their further validation as parental material in breeding.
Gut inflammation associated with age and Alzheimer’s disease pathology: a human cohort study
Authors: Heston Margo B. , Hanslik Kendra L. , Zarbock Katie R. , Harding Sandra J. , Davenport-Sis Nancy J. , Kerby Robert L. , Chin Nathaniel , Sun Yi , Hoeft Ana , Deming Yuetiva , Vogt Nicholas M. , Betthauser Tobey J. , Johnson Sterling C. , Asthana Sanjay , Kollmorgen Gwendlyn , Suridjan Ivonne , Wild Norbert , Zetterberg Henrik , Blennow Kaj , Rey Federico E. , Bendlin Barbara B. , Ulland Tyler K.
Published: November 2023

Age-related disease may be mediated by low levels of chronic inflammation (“inflammaging”). Recent work suggests that gut microbes can contribute to inflammation via degradation of the intestinal barrier. While aging and age-related diseases including Alzheimer’s disease (AD) are linked to altered microbiome composition and higher levels of gut microbial components in systemic circulation, the role of intestinal inflammation remains unclear. To investigate whether greater gut inflammation is associated with advanced age and AD pathology, we assessed fecal samples from older adults to measure calprotectin, an established marker of intestinal inflammation which is elevated in diseases of gut barrier integrity. Multiple regression with maximum likelihood estimation and Satorra–Bentler corrections were used to test relationships between fecal calprotectin and clinical diagnosis, participant age, cerebrospinal fluid biomarkers of AD pathology, amyloid burden measured using11C-Pittsburgh compound B positron emission tomography (PiB PET) imaging, and performance on cognitive tests measuring executive function and verbal learning and recall. Calprotectin levels were elevated in advanced age and were higher in participants diagnosed with amyloid-confirmed AD dementia. Additionally, among individuals with AD dementia, higher calprotectin was associated with greater amyloid burden as measured with PiB PET. Exploratory analyses indicated that calprotectin levels were also associated with cerebrospinal fluid markers of AD, and with lower verbal memory function even among cognitively unimpaired participants. Taken together, these findings suggest that intestinal inflammation is linked with brain pathology even in the earliest disease stages. Moreover, intestinal inflammation may exacerbate the progression toward AD.
Rapid detection of residual chlorpyrifos and pyrimethanil on fruit surface by surface-enhanced Raman spectroscopy integrated with deep learning approach
Authors: Chen Zhu , Dong Xuan , Liu Chao , Wang Shenghao , Dong Shanshan , Huang Qing
Published: November 2023

Chlorpyrifos and pyrimethanil are widely used insecticides/fungicides in agriculture. The residual pesticides/fungicides remaining in fruits and vegetables may do harm to human health if they are taken without notice by the customers. Therefore, it is important to develop methods and tools for the rapid detection of pesticides/fungicides in fruits and vegetables, which are highly demanded in the current markets. Surface-enhanced Raman spectroscopy (SERS) can achieve trace chemical detection, while it is still a challenge to apply SERS for the detection and identification of mixed pesticides/fungicides. In this work, we tried to combine SERS technique and deep learning spectral analysis for the determination of mixed chlorpyrifos and pyrimethanil on the surface of fruits including apples and strawberries. Especially, the multi-channel convolutional neural networks-gate recurrent unit (MC-CNN-GRU) classification model was used to extract sequence and spatial information in the spectra, so that the accuracy of the optimized classification model could reach 99% even when the mixture ratio of pesticide/fungicide varied considerably. This work therefore demonstrates an effective application of using SERS combined deep learning approach in the rapid detection and identification of different mixed pesticides in agricultural products.
Assessment of eruption source parameters using infrasound and plume modelling: a case study from the 2021 eruption of Mt. Etna, Italy
Authors: De Angelis Silvio , Zuccarello Luciano , Scollo Simona , Mereu Luigi
Published: November 2023

Atmospheric injection of volcanic ash during eruptions is a threat to aviation. Reliable forecast of airborne ash dispersal relies on empirical and numerical models. Key inputs into these models are so-called eruption source parameters such as the rate at which pyroclastic material is ejected from the vent and the maximum height of eruptive columns. Here, we use infrasound data recorded during eruptive activity in June 2021 at Mt. Etna, Italy, to demonstrate its potential for assessment of eruption rates in near-real time. We calculate a time series of flow velocity at the vent using data corrected for topographic scattering, and the effect of vent geometry on the acoustic source radiation. We obtain values of flow velocity of 50–125 m/s during a period of sustained, paroxysmal, activity. We use independent estimates from other ground-based remote sensing data to validate our results. Further, we use the infrasound-derived flow velocities as input into a 1D plume model to estimate the maximum height of the eruption column. Our results suggest that infrasound technology holds promise to assess eruption rates and inform modelling of volcanic plumes. We anticipate that implementation of real-time operational workflows based on infrasound data and plume modelling will impact decision-making and risk mitigation at active volcanoes.
Systematic review and network meta-analysis of efficacy and safety of interventions for preventing anti-tuberculosis drug induced liver injury
Authors: Akkahadsee Pattaraporn , Sawangjit Ratree , Phumart Panumart , Chaiyakunapruk Nathorn , Sakloetsakun Duangkamon
Published: November 2023

Anti-tuberculosis drug induced liver injury (Anti-TB DILI) is the most common adverse events (AEs) necessitating therapy interruption but there is no preventing regimen. This study aimed to examine the efficacy and safety of herbs/alternative medicines for preventing anti-TB DILI. Relevant articles were identified through a systematic search in 5 international databases from inception till March 2022. All randomized controlled trials (RCT) assessing the effects of herbal or alternative medicines against anti-TB DILI were included. The network meta-analysis (NMA) was used to synthesize the evidence for preventing hepatotoxicity using a random-effects model. A total of 3423 patients from 14 RCTs were included. The NMA indicated that supplementation of Turmeric plus Tinospora cordifolia (RR 0.07; 95% CI 0.02 to 0.28), and N-acetyl cysteine (NAC) (RR 0.09; 95% CI 0.01 to 0.75) significantly reduced the incidence of anti-TB DILI compared with placebo. In addition, poly herbal product significantly reduced alkaline phosphatase (ALP) (MD − 21.80; 95% CI − 33.80 to − 9.80) and total bilirubin (Tbil) compared with placebo (MD − 0.51; 95% CI − 0.76 to − 0.26). There was no statistically significant difference in the occurrence of AEs in any intervention. In conclusion, Turmeric plus Tinospora cordifolia, NAC and poly-herbal product may provide benefit for preventing anti-TB DILI in TB patients. However, these findings are based on a small number of studies. Additional studies are warranted to confirm the findings.
The expanding range of emerging tick-borne viruses in Eastern Europe and the Black Sea Region
Authors: Ergunay Koray , Bourke Brian P. , Reinbold-Wasson Drew D. , Nikolich Mikeljon P. , Nelson Suppaluck P. , Caicedo-Quiroga Laura , Vaydayko Nataliya , Kirkitadze Giorgi , Chunashvili Tamar , Long Lewis S. , Blackburn Jason K. , Cleary Nora G. , Tucker Cynthia L. , Linton Yvonne-Marie
Published: November 2023

We analysed both pooled and individual tick samples collected from four countries in Eastern Europe and the Black Sea region, using metagenome-based nanopore sequencing (NS) and targeted amplification. Initially, 1337 ticks, belonging to 11 species, were screened in 217 pools. Viruses (21 taxa) and human pathogens were detected in 46.5% and 7.3%, respectively. Tick-borne viral pathogens comprised Tacheng Tick Virus 2 (TTV2, 5.9%), Jingmen Tick Virus (JMTV, 0.9%) and Tacheng Tick Virus 1 (TTV1, 0.4%). An association of tick species with individual virus taxa was observed, with the exception of TTV2, which was observed in both Dermacentor and Haemaphysalis species. Individual ticks from pools with pathogen detection were then further screened by targeted amplification and then NS, which provided extensive genome data and revealed probable pathogen Haseki Tick Virus (HTV, 10.2%). Two distinct TTV2 clades were observed in phylogenetic analysis, one of which included closely related Dermacentor reticulatus Uukuviruses. JMTV detection indicated integrated virus sequences. Overall, we observed an expansion of newly documented pathogenic tick-borne viruses into Europe, with TTV1 being identified on the continent for the first time. These viruses should be included in the diagnostic assessment of symptomatic cases associated with tick bites and vector surveillance efforts. NS is shown as a useful tool for monitoring tick-associated pathogens in pooled or individual samples.
The impact of food availability on tumorigenesis is evolutionarily conserved
Authors: Tissot Sophie , Guimard Lena , Meliani Jordan , Boutry Justine , Dujon Antoine M. , Capp Jean-Pascal , Tökölyi Jácint , Biro Peter A. , Beckmann Christa , Fontenille Laura , Do Khoa Nam , Hamede Rodrigo , Roche Benjamin , Ujvari Beata , Nedelcu Aurora M. , Thomas Frédéric
Published: November 2023

The inability to control cell proliferation results in the formation of tumors in many multicellular lineages. Nonetheless, little is known about the extent of conservation of the biological traits and ecological factors that promote or inhibit tumorigenesis across the metazoan tree. Particularly, changes in food availability have been linked to increased cancer incidence in humans, as an outcome of evolutionary mismatch. Here, we apply evolutionary oncology principles to test whether food availability, regardless of the multicellular lineage considered, has an impact on tumorigenesis. We used two phylogenetically unrelated model systems, the cnidarian Hydra oligactis and the fish Danio rerio, to investigate the impact of resource availability on tumor occurrence and progression. Individuals from healthy and tumor-prone lines were placed on four diets that differed in feeding frequency and quantity. For both models, frequent overfeeding favored tumor emergence, while lean diets appeared more protective. In terms of tumor progression, high food availability promoted it, whereas low resources controlled it, but without having a curative effect. We discuss our results in light of current ideas about the possible conservation of basic processes governing cancer in metazoans (including ancestral life history trade-offs at the cell level) and in the framework of evolutionary medicine.
Exposure to climate change information predicts public support for solar geoengineering in Singapore and the United States
Authors: Rosenthal Sonny , Irvine Peter J. , Cummings Christopher L. , Ho Shirley S.
Published: November 2023

Solar geoengineering is a controversial climate policy measure that could lower global temperature by increasing the amount of light reflected by the Earth. As scientists and policymakers increasingly consider this idea, an understanding of the level and drivers of public support for its research and potential deployment will be key. This study focuses on the role of climate change information in public support for research and deployment of stratospheric aerosol injection (SAI) in Singapore (n = 503) and the United States (n = 505). Findings were consistent with the idea that exposure to information underlies support for research and deployment. That finding was stronger in the United States, where climate change is a more contentious issue, than in Singapore. Cost concern was negatively related to support for funding and perceived risk was negatively related to support for deployment. Perceived government efficacy was a more positive predictor of support for funding in Singapore than in the United States. Additionally, relatively low support for local deployment was consistent with a NIMBY mindset. This was the first study to quantify the role of climate change information in SAI policy support, which has practical implications for using the media and interpersonal channels to communicate about SAI policy measures.