bluuebunny/crossref_metadata_2025 huggingface dataset:
Acupuncture Ameliorates Depression-like Behaviors through Modulating the Neuroinflammation Mediated by TLR4 Signaling Pathway in Rats Exposed to Chronic Restraint Stress
Authors: Jiang Huili , Long Xianqi , Wang Yu , Zhang Xuhui , Chen Lu , Yang Xinjing , Zhao Bingcong , Zhang Ye , Chai Yemao , Bao Tuya
Published: June 2023

Recently, emerging evidence has identified that stress-induced activation of neuroinflammation is considered to be one of the most prevalently precipitating factors in the pathogenesis of depression. Data from clinical trials and experimental findings has verified the efficacy and safety of acupuncture in the treatment of depression. However, the mechanism of the antidepressant effect of acupuncture has not been fully elucidated. The current study aimed to investigate the antidepressant effect and mechanism of acupuncture through modulating the neuroinflammation mediated by toll-like receptor 4 (TLR4) signaling pathway in rats exposed to chronic restraint stress (CRS). All rats were subjected to CRS for 21 days, with the exception of rats in control group. One hour before CRS, rats in acupuncture group were exposed to acupuncture at Baihui (GV20) and Yintang (GV29). The depression-like behaviors were evaluated by body weight assessment and sucrose preference test at 0, 7, 14, 21 days. The expression of activated microglia in hippocampus was detected by immunofluorescence. The expression of key proteins on TLR4 signaling pathway of TLR4, MyD88, TRAF6, NF-κB p65, TNF-α and mRNA of TLR4 in the hippocampus were detected by western blot and real-time quantitative polymerase chain reaction to investigate the effect of acupuncture on stress-induced activation of neuroinflammation. The present study provided evidence that acupuncture exerted potential antidepressant-like effect that might be mediated in part by suppressing the neuroinflammation induced by TLR4 signaling pathway, which may be a promising treatment target to improve current treatments for depression.
The Inter-dependence of the Limbs of Feedback Loops Affects the Regulation and Homeostasis of Multiple Physiological Systems.
Authors: Fitzgerald Stephen , Bean Nigel , Falhammar Henrik , Hoermann Rudolf , Kohanim Yael Korem , Pohlabeln Hermann , Beverborg Niels Grote
Published: June 2023

The fundamental models of physiological regulation and homeostasis remain uncertain and controversial. We aimed to derive quantitative evidence regarding these models from the study of population data of balance points of different parameters and their respective controlling hormones in terms of the slopes of correlations between parameters and controlling hormones, and the slopes of derived estimates of the physiological responses of parameters to their respective regulating hormones. Our simulations showed that changes to the population distribution of, or dependence between, feedback loop limbs alter these slopes of population data derived correlations and estimates in specific ways. Our corresponding empirical findings were similar across multiple systems and support a general model of regulation whereby the balance points of parameters are generated peripherally under the influence of feed-back loops, both limbs of which may interdependent. This interdependence potentially bestows evolutionary homeostatic and regulatory advantages.
Near real-time detecting of wildfire using MODIS and NOAA/AVHRR data
Authors: Aghazadeh Firouz
Published: June 2023

Forest fire in recent years has given much attention to climate change and ecosystem. Detection of fire in Near real time is necessary to prevent large-scale casualties. Remote sensing is a quick and inexpensive way to detect and monitor forest fires on a large scale. The purpose of this research is to identify forest and rangeland fire using MODIS and AVHRR sensors in Kayamaki Wildlife Refuge. In order to carry out research, the dates of the fire occurred at MODIS products were recorded. Then the images of both sensors were prepared based on the date of the fires. After preprocessing the images, different fire detection algorithms (i.e. IGBP, Giglio, Extended, Dynamic for NOAA/AVHRR images and Giglio, Extended for MODIS /AQUA &TERRA images) for fire detection were investigated. The results of fire detection algorithms with MODIS products were there by evaluated. The results showed that among different algorithms, the dynamic algorithm on NOAA/AVHRR images is more suitable than the other ones with a low error rate of 28% for fire detection. Although the IGBP algorithm has a lower error rate relative to the dynamic algorithm, based on the characteristics (using NDVI, the use of two images (D1, D2) to detect fire in Near real time, the thresholds for removal of wrong alarms, etc.) has a dynamic algorithm, but the IGBP algorithm shows incorrect alarms despite the low error rate and the Giglio algorithm on MODIS/TERRA images were determined as favorable algorithm with 28% error. This is confirmed in the fires that were detected in terms of location relative to the fire locations found in the MODIS products. Also, MODIS and AVHRR sensors were compared in terms of Near real-time fire detection ability. AVHRR detected the highest number of fires (68.25%) with low error rate (31.75%) and MODIS discovered lower fire numbers (46.5%) and high error rate (53.5%).
Comparison of three methods for determination of nitrate content in different vegetables
Authors: Beheshti Mehdi , Hasheminasab Kobra Sadat , Shahbazi Karim , Tavanamehr Ainaz , Cheraghi Meysam
Published: June 2023

Vegetables are the major source of dietary nitrate, with a wide range of nitrate content. The presence of nitrate in vegetables is often associated with harmful effects on human health, i.e. with toxic effects of methemoglobinemia and the possibility of causing an endogenous formation of carcinogenic N-nitroso compounds. Therefore, accurate measurement of nitrate in these products is of great importance. Various analytical techniques have been developed to determine nitrate in biological samples and vegetables. The purpose of this study is to compare the three methods BS EN 12014-2 (HPLC UV/Vis), ISO 6635, and Cd-column for nitrate measurement and to introduce an accurate, safe, and useful method for nitrate measurement in 11 important vegetables with a wide range of nitrate content. The results of this study showed strong agreement between the three methods. A strong and significant regression was observed between the HPLC run using ISO 6635 and the HPLC method using the Cd-column, with the slope of the regression curve close to 1 for most of the vegetable samples. Between HPLC and ISO 6635 methods, the fitted regression curve had slopes as high as 1.0946 and as low as 0.9195, respectively. While, the highest and lowest slopes of the fitted regression curve between HPLC and the Cd-column methods were 1.088 and 0.9217, respectively. The highest slope between the two methods ISO 6635 and Cd-column was equal to 1.0832 and the lowest was equal to 0.8421. The correlation coefficient of the regression equation was significant at a level of 99% (p ˂ 0.01) across all samples and correlations of linear regression. The results of this study showed that all three methods have high accuracy for nitrate measurement in vegetables. However, the type of sample and the accessibility of the facility plays a big role in the selection of an analytical method. The HPLC method is slower than the Spectrophotometry methods (ISO 6635 and Cd-column), despite having a higher initial investment cost. However, it has a lower risk of environmental pollution and health risk for the staff. Spectrophotometry methods have high speed, and the initial cost of equipment is lower, but they have more environmental risk and are labor-intensive than the HPLC method. Finally, despite the fact that the compared methods were very accurate at measuring nitrate in vegetables, the HPLC method is suggested because it is reliable, secure, and practical in vegetables due to the figure of merits mentioned.
SARS-CoV-2 infection as a model to study the effect of cinnamaldehyde as adjuvant therapy for viral pneumonia.
Authors: Vezzani Bianca , Perrone Mariasole , Carinci Marianna , Tombolato Alberto , Tombolato Denis , Gentili Valentina , Rizzo Roberta , Campo Gianluca , Morandi Luca , Papi Alberto , Spadaro Savino , Contoli Marco , Pinton Paolo , Giorgi Carlotta , Casolari Paolo , Daminato Claudio
Published: June 2023

Introduction:
The recent pandemic outbursts, due to SARS-CoV-2, has highlighted once more the central role of the inflammatory process in the propagation of viral infection. In fact, the main consequence of COVID-19 is the induction of a diffuse pro-inflammatory state, also defined as cytokine storm, that affects different organs, but mostly the lungs. Therefore, despite the progress in the vaccination campaign, the high mutability of this virus also requires effective pharmaceutical approaches aimed to reduce the inflammatory spread.
Methods
The effect of SARS-CoV-2 infection on IL-1β and IL-6 levels was assessed by performing ELISA on patients' serum and stimulated-PBMCs. The therapeutic potential of cinnamaldehyde on COVID-19 was evaluated in vitro, testing the effects on THP-1 macrophages' inflammatory state, and on the viral replication in Calu-3 and Vero6 cells. Moreover, we assessed the effect of cinnamaldehyde also in vivo generating a lung-inflammatory model by intranasal administration of LPS.
Results
In this study we highlight that COVID-19 patients have higher IL-1β and IL-6 plasma levels compared to non-COVID-19 pneumonia patients, showing that human mononuclear cells (PBMCs) isolated from SARS-CoV-2 infected patients are more prone to release pro-inflammatory cytokines upon stimuli. Furthermore, we demonstrated how cinnamaldehyde, a natural compound easily tolerated by the majority of human beings, significantly reduces SARS-CoV-2-related inflammation by inhibiting IL-1β release by macrophages, and viral replication in epithelial cells. In addition, we confirmed the ability of aerosol-administered cinnamaldehyde to in vivo reduce IL-1β release in a lung-inflammatory model.
Conclusion
The obtained results suggest the possible use of cinnamaldehyde suitable as a co-adjuvant preventive treatment for COVID-19 disease together with vaccination, but also as a promising dietary supplement to reduce, more broadly, viral induced inflammation.
Association between the level of concern for COVID-19 and information sources among college students
Authors: Peprah Emmanuel , Amesimeku Etornam , Chherti Himani , Ruan Christina , Fordjuoh Judy , Wang Cong , Patena John , Vieira Dorice , Ryan Nessa , Iloegbu Chukwuemeka , Gyamfi Joyce , Odumegwu Jonathan
Published: June 2023

Objective We administered an online survey to 185 college students to assess their level of concern, information sources, and mitigation strategies during the COVID-19 pandemic in 2021. Responses from only students enrolled in college during 2021 were analyzed. We sought to examine their level of concern as defined as utilization of protective measures and/or personal protective equipment and/or social distancing and/or following other public health recommendations from public health sources.
Results College students that participated in our survey used various sources to obtain information about COVID-19 that included social media and other sources (58.92%), followed by mainstream media (27.57%) and email from their university/college (13.51%). Students who learned about the pandemic from social media (65.94%) were concerned; however, only 23.19% and 10.87% of the participants that sourced information from mainstream media and their schools’ email, respectively, were concerned. Furthermore, students who received information from social media and other sources were three times more likely to be concerned about COVID-19 than students who received information from the university via email.
New insights on habitat preferences of the Northern Emerald dragonfly (Somatochlora arctica)
Authors: Cristofaro Luigi , Batty Patricia , Muir Daniele , Law Alan
Published: June 2023

Odonates are widely considered to be bioindicators of freshwater habitat quality. Somatochlora arctica (Corduliidae) are commonly found across Eurasia, predominantly North and Central Europe, but in the UK, they is listed as near threatened and currently restricted to few areas of Scotland despite a large, potential habitat availability. Across their range their larvae are commonly found in Sphagnum-filled bog pools nearby coniferous woodlands, but detailed data on their habitat preferences are limited which inhibits conservation efforts. We designed a survey targeting 3 known, but geographically distinct, S. arctica locations in Scotland: Abernethy & Loch Garten National Nature Reserve (NNR), Beinn Eighe NNR and Flanders Moss NNR to evaluate how different environmental factors (e.g. water depth and chemistry, bryophyte species and coverage, woodland distance, etc.) affect the presence and abundance of S. arctica. We found pools that were located far from woodlands (i.e. >50m) had a lower presence of S. arctica and that S. arctica was mostly found in pools with high densities of Sphagnum coverage (> 90%). Our results highlight the importance of nearby woodlands and Sphagnum quantity and potentially species type for different life stages of S. arctica. Future conservation projects should consider the proximity of woodland to current and restored S.arctica sites or promoting expansion of surrounding natural woodland patches in order to benefit S.arctica and potentially other odonates.
Multidrug-resistant Gram-negative bacterial colonization in patients, carriage by healthcare workers and contamination of hospital environments in Ghana
Authors: Yeboah Esther Eyram Asare , Agyepong Nicholas , Amoako Daniel Gyamfi , Abia Akebe Luther King , Owusu-Ofori Alexander , Essack Sabiha Yusuf
Published: June 2023

Purpose
Patients already colonized with multidrug-resistant (MDR) Gram-negative bacteria (GNB) on admission to critical care units may be an important source of their transmission in hospitals. We sought to determine the prevalence of MDR GNB colonization in patients, staff, and the ward environment and to assess the risk factors for colonization of patients.
Methods
The study was conducted from April 2021 to July 2021 in a teaching hospital in Ghana. MDR GNB were isolated from rectal and hand swabs of patients on admission and after 48 hours. Swabs from HCW’s hands and the ward environment were also taken. Risk factors for colonization with MDR GNB were assessed using univariate and multivariate analysis.
Results
MDR GNB rectal colonization rate among patients was 50.62% on admission and 44.44% after 48 hours. MDR GNB were isolated from 6 (5.26%) and 24 (11.54%) of HCW’s hand swabs and environmental swabs, respectively. Escherichia coli and Klebsiella pneumoniae were the most frequently isolated MDR. Previous hospitalization (p-value = 0.021, OR,95% CI = 7.170 (1.345–38.214) was significantly associated with colonization by MDR GNB after 48 hours of admission. Age (21–30 years) (p-value = 0.022, OR, 95% CI = 0.103(0.015–0.716) was significantly identified as a protective factor associated with a reduced risk of rectal MDR GNB colonization.
Conclusions
The high colonization of MDR GNB in patients and carriage of MDR GNB on HCW’s hands and contamination of environments, highlights the need for patient screening, and stringent infection prevention and control practices to prevent the spread of MDR GNB in hospitals.
Dietary thiamine requirement of Pacific white shrimp, Litopenaeus vannamei
Authors: Wijemanna Udith , Lee Kyeong-Jun
Published: June 2023

This study investigated the dietary thiamine (vitamin B1) requirement of Pacific white shrimp (Litopenaeus vannamei) for optimal growth performance, feed utilization, and innate immunity. Six test diets were formulated to contain 3.32, 20.8, 38.7, 60.2, 80.7, and 108 mg/kg of graded thiamine levels (designated as T0, T20, T40, T60, T80, and T100, respectively). A total of 360 shrimp (6.03 ± 0.03 g) were randomly assigned into 24 acryl tanks (240 L) to be quadruplicated per treatment, and feeding was done in six equal portions daily for 58 days. The T20 diet fed group exhibited significantly higher growth performance compared to shrimp fed T0 and T100 diets. Feed conversion ratio and protein efficiency ratio were not significantly different among experimental groups. A significantly higher phenoloxidase activity was exhibited in T20 dietary group than T0 and T100 groups. Lysozyme activity was significantly enhanced in T20, T40, T60, and T80 groups than in T0 group and antiprotease activity was not significantly affected. Antioxidant parameters: superoxide dismutase activity and glutathione peroxidase activities were significantly elevated in T20 dietary treatment group. Significantly upregulated proPO gene expression was observed in T20 and T40 dietary treatment groups, although penaidine 3a and insulin-like growth factor-1 relative gene expressions were not significantly different. Glucose, total cholesterol, triglyceride, and total protein levels in hemolymph were not significantly different. Intestinal histology observations did not show significant differences in villi heights. A piecewise regression of weight gain percentage (WG%) estimates the optimal dietary thiamine requirement of L. vannamei at 19.7 mg/kg.
Systematic Analysis Of Expression Profiles Of SRSF Family Members For Prognostic Application In DLBCL
Authors: Wang Zanzan , Zhang Jiawei , Yan Xiao , Sheng Lixia , Ouyang Guifang
Published: June 2023

Diffuse large B-cell lymphoma (DLBCL) is the most frequent subtype of non-Hodgkin’s lymphoma. Serine and arginine-rich splicing factors (SRSF) family is an important splicing regulator and plays vital roles in RNA nuclear transportation and RNA stability. However, the role of the SRSF family in DLBCL is still unknown. The GEPIA database and the Human Protein Atlas were used to investigate the differential expression of SRSF in the DLBCL tissue and corresponding normal tissues. The mRNA expression profiles and clinicopathological data from the GSE10846 dataset from the Gene Expression Omnibus (GEO) database were downloaded to explore the clinical significance, prognostic value, and tumor immune microenvironments in DLBCL. SRSF family expression levels were substantially upregulated in DLBCL compared with normal tissues. Increased SRSF mRNA expression levels were associated with poorer overall survival. We selected 4 SRSF genes (SRSF1, SRSF7, SRSF10, and SRSF12) related to prognosis to conduct a risk model. Additionally, SRSF is strongly associated with MMR genes, DNA methyltransferase, and tumor stemness index. SRSF expression was closely correlated with infiltrating levels of specific types of immune cells in DLBCL, especially B cells naïve and T cell CD4 memory activated. Pathway enrichment analysis indicated that the SRSF family was involved in the upregulation of tumor metabolism and metastasis. Finally, SRSF protein was higher in DLBCL cell lines than in control cells by western blot. SRSF family genes have complicated roles in tumorigenesis, progression, and prognosis in DLBCL.
Subtle transcriptomic response of Eurasian perch (Perca fluvatilis) associated with Triaenophorus nodulosus plerocercoid infection
Authors: Taube Konrad , Noreikiene Kristina , Kahar Siim , Gross Riho , Ozerov Mikhail , Vasemägi Anti
Published: June 2023

BackgroundDetermining the physiological effects of parasites and characterizing genes involved in host responses to infections are essential to improving our understanding of host-parasite interactions and their ecological and evolutionary consequences. This task, however, is complicated by high diversity and complex life histories of many parasite species. The use of transcriptomics in the context of wild-caught specimens can help ameliorate this by providing both qualitative and quantitative information on gene expression patterns in response to parasites in specific host organs and tissues. Here, we evaluated the physiological impact of the widespread parasite, the pike tapeworm (Triaenophorus nodulosus),on its second intermediate host, the Eurasian perch (Perca fluviatilis).MethodsWe used an RNAseq approach to analyse gene expression in the liver, the target organ ofT. nodulosusplerocercoids, and spleen which is one of the main immune organs in teleost fishes. We compared perch collected from multiple lakes with individuals with (n = 8) and without (n = 6)T. nodulosusplerocercoids in the liver.ResultsResults revealed a small number of differentially expressed genes (DEGs, adjusted p-value ≤ 0.05) in both spleen (n = 22) and liver (n = 10). DEGs in spleen consisted of mostly upregulated immune related genes (e.g.,JUN,SIK1,THSB1), while those in the liver were often linked to metabolic functions (e.g.,FABP1,CADM4, CDAB). However, Gene Ontology (GO) analysis showed lack of functional enrichment among DEGs.ConclusionsThis study demonstrates that Eurasian perch displays a subtle response at a gene expression level toT. nodulosus, which together with earlier work, suggests that moderateT. nodulosusplerocercoid infection most likely does not provoke an extensive host immune response and have relatively low physiological costs for the host. Our findings illustrate that not all conspicuous infections have severe effects on host gene regulation.
Natural factors or human disturbance: What shapes the occurrence of black grouse Lyrurus tetrix on the edge of its continuous range?
Authors: Adamowicz Michał , Gortat Tomasz , Czortek Patryk , Chiliński Michał
Published: June 2023

The Eurasian black grouse belongs to a group of birds sensitive to environmental changes. A decrease in its numbers may be a signal of the deteriorating habitat condition, making this species an important indicator of shifts the in functioning of ecosystems. In this study, we analyzed the impact of a number of predictors on the occurrence of the black grouse in one of its last Central European, mountainous refuge - Polish Tatra Mountains. The aim of the study was to understand the requirements of the species and identify the most important factors for its survival. We found that the existence of black grouse depends on specific proportions between natural factors shaping the local environment – mainly the type of vegetation land cover. Smaller, but important in the context of species protection, is the negative impact of human disturbance. The effect of analyzed factors on the presence of the black grouse occurs not only in the areas inhabited by the birds but also in their surroundings. The presence of black grouse positively correlated with vegetation providing food and lekking sites and land cover by forest in the surrounding. A negative relationship was found with bare rock cover and high tourist pressure around the occurrence sites. Our results indicate that for black grouse protection, it is necessary to maintain natural vegetation cover and open areas, and reduce tourist pressure. It is also important to protect adjacent areas that meet the species’ habitat requirements, in order to avoid fragmentation of the population.
Pre-Marital Sexual Debut and Associated Factors among Unmarried Regular Students of AdigratUniversity, Northern Ethiopia
Authors: Gebremariam Guesh , Adhanom Gebre , Hagos Selemun , Berihu Tesfay
Published: June 2023

Background Premarital sexual debuts are increasing in the globe. It has vital role in spreading sexually transmitted diseases and unwanted pregnancy among the youths. Even though a single study was conducted among high school students of Adigrat town, there is no published data that describes premarital sex and factor associated among students of Adigrat University.Thus, this study has aimed to assess pre-marital sexual debut and associated factors among unmarried regular studentsof AdigratUniversity, northern Ethiopia. Methods Institutional based cross-sectional study was conducted using a facilitator guided self-administered questionnaire among students of Adigrat University. Study participants were recruited using multistage sampling technique. Data was analyzed using statistical package for social science version 22 to determine the association between variables. A p-value < 0.05 with a corresponding 95% confidence interval was considered as statistically significant. Result The overall prevalence of premarital debut among the study participants was,22.9%.According to multivariate analysis, peer pressure (AOR = 1.8, 95% CI: 1.2–2.6, p = 0.002); watching pornographic video (AOR = 1.6, 95% CI: 1.1–2.3, p = 0.031) and using psychoactive drugs (substance) (AOR = 2.0, 95% CI: 1.3–3.1, p = 0.001) were statistically significant factors associated with the occurrence of pre-marital sexual debut among the study participants. Conclusion Premarital sexual debut is becoming more prevalent among students. Watching pornographic video, peer pressure and using psychoactive drugs were statistically contributing factors to debut student in premarital sex. Hence, our finding suggests ministry of health and ministry of education should work together giving more attention for designing persistent and effective reproductive health education programme among students of higher education.
Additive Manufacturing of Hierarchically Porous Silicon-based Ceramics
Authors: Gupta Vipul , Norouzi Ahmad , Paull Brett
Published: June 2023

Hierarchically porous silicon-based ceramics present unique opportunities for new health, energy, aerospace, and defence applications. However, their manufacturing has always been challenging, limiting their wider adoption and demonstrating their true potential. Manufacturing these materials through conventional templating and etching processes has been impractical, and modern additive manufacturing of hierarchically porous silicon-based ceramics has not been realised. Here we show a new resin system to allow 3D printing of controlled porosity silicon-based ceramics using low-cost and high-resolution photo-polymerisation-based printing methods. The method has enabled printing of three-dimensional complex micro-structures, such as microneedle arrays, and the intimate mimicking of leaves and insect wings, with controlled unimodal, bimodal, and trimodal porosity black glass. It has also been used to develop high-resolution multi-material structures with different porosities integrated into a unibody design using the standard single-material digital light projection-based printers. The approach can be extended to other binary, tertiary, or quaternary silicon-based ceramics. This work allows the production of bespoke macrostructures with three-dimensionally controlled microfeatures, incorporating an hierarchical series of pores, from a few micrometres to sub-nanometre-sized, delivering unique structure and function relationships and paving the way for the next generation of nature-inspired energy generators, photonic crystals, catalytic reactors, sorbents, filters, and medical implants.
All-p ZVLL Inverter with Bootstrapping based on a ditch OTFT
Authors: Gupta Sakshi , Mittal Poornima , Juneja Pradeep
Published: June 2023

This research manifests the characteristics both static and dynamic, of a bootstrapped all-p ZVLL inverter using a single gate BGBC organic transistor with extra p + doping near the source and drain electrodes and a 40 nm ditch incorporated in the pentacene organic semiconductor layer. Several topologies of the inverter were experimented with and compared in terms of their performance pre and post the application of the bootstrap technique. Bootstrapping proved to be a befitting choice for obtaining the maximum efficient results in terms of noise margin, delay in propagation, transient time, and gain. Upon comparison it was observed that the bootstrapped inverter showed considerably better voltage transfer characteristics in terms of high and low noise margins. A surge of 18.46% and 22.25% is noticed in noise margins of the bootstrapped device as compared to the device without bootstrapping. Also, the dynamic response of DLL and ZVLL topology devices exhibit a hike in the voltage swing by 24% and 13% respectively. Furthermore, contrast to DLL, the average delay of the ZVLL inverter decreases by 54.5 µs after the application of bootstrapping technique, whereas the value of average propagation delay decreases by a mere 10 µs. Pertaining to the results attained, ZVLL proves to be a more responsive circuit, leading to an inverter that is apparently more robust, better noise margins, attenuated power leakage and improved sensitivity.
Atypical phenotypes and novel OCRL variations in Southern Chinese patients with Lowe syndrome
Authors: Du Rong , Lin Yunting , Li Tong , Xu Aijing , Huang Yonglan , Mei Huifen , Huang Xiaoli , Tan Dongdong , Zheng Ruidan , Liang Cuili , Cai Yanna , Shao Yongxian , Zhang Wen , Liu Li , Zeng Chunhua
Published: June 2023

Background:Lowe syndrome is an uncommon genetic disorder that follows an X-linked recessive inheritance pattern. It is defined by the occurrence of congenital cataracts, psychomotor retardation, and dysfunctional proximal renal tubules. This study examined the clinical and genetic features of eight children diagnosed with Lowe syndrome in Southern China.Methods:Whole-exome sequencing was performed on eight Lowe syndrome patients from three medical institutes in Southern China, and clinical and genetic data were collected and analyzed retrospectively.Results:In our cohort, the clinical symptoms of the eight Lowe syndrome individuals varied. One patient was diagnosed with Lowe syndrome but did not have congenital cataracts. All patients had psychomotor retardation, short stature, low molecular weight proteinuria, and albuminuria. The clinical characteristics also included elevated creatine kinase (CK)/ aspartate aminotransferase (AST)/ lactate dehydrogenase (LDH) (87.5%), cryptorchidism (66.7%), renal rickets (37.5%), renal tubular acidosis (37.5%), phosphaturia (37.5%), hypercalciuria (37.5%), nephrocalcinosis (25%) and glycosuria (25%). Eight variations inOCRLwere identified in all eight patients with Lowe syndrome, involving three known and five novel variations. All variations are located in exons 8-23 and occur in functional domains. Three novel nonsense variations were classified as pathogenic. Two patients with novel missense variations classified as uncertain significance showed typical severe phenotypes.Conclusion:This study describes the first case of an atypical Lowe syndrome patient without congenital cataracts in China and identifies novelOCRLgene variants, which broadens the genetic and symptomatic range for Lowe syndrome.
Appearance, molecule identification, and phylogenetic analysis of the three Leonurus species used for the treatment of gynecological diseases
Authors: Du Qing , Rong Ziyi , Zhang Chang , Wang Liqiang , Guan Yanjuan , Chen Haimei , Wang Bin , Liu Chang
Published: June 2023

We analyzed and compared the three Leonurus species from appearance characteristics, intrinsic gene contents and functions, gene transcribed spacer regions (ITS2) in the nuclear genome, and the process of evolutionary development. The chloroplast genomes of them were found to be common circular structures with the four regions, the length range from 151236bp to 151689bp including the identical number of 37 tRNA and 8 rRNA. The special trans-spliced rps12 genes with two introns and three exons can be caught sight of L. sibiricus. The isoleucine (AUU) and cysteine (UGC) was the most and least abundant amino acid with the codons ending with most bases of A/U. The eleven hotspot divergent regions and four specific CDS genes were systematically counted with the highest nucleotide diversity. We cloned the DNA sequences of the two chosen IGS regions (atpH-atpI and rps15-ycf1) to develop the DNA barcodes, thus found that sixteen specific variable SNP sites and two Indel sites within the three Leonurus species. Furthermore, the ITS2 DNA sequences of 7 Leonurus species were varied after the 57th base in a total of 221 bases. In the aftermath of evolutional analysis, the three Leonurus species were significantly clustered into one great clade, while they were located at the different sub-branch with similar topology and close relationship of Phlomoides rotata based on the sequences of 64 shared nucleotide and ITS2 DNA. Thus above results can directly offer various evidence to better clarify the specific distinction among the three Leonurus species and their evolutionary history.
Clinical Features and Short-Term Outcomes in Virus-Induced Bradycardia: Comparison Between Covid and non-Covid Infected Patients
Authors: Scuotto Frederico , Marques Mayara Maza , Rodrigues Daniela Pereira , Parravano Daniella Cardoso , Coutinho Enia Lucia , Cirenza Claudio
Published: June 2023

Covid-19 outbreak has drawn attention to the fact that viral infections might present with clinical bradycardia. Seeking its clinical significance, not yet unveiled by the literature, we come across other viral infections that also show clinical bradycardia during its clinical course, such as dengue fever and viral diarrhea. The clinical presentation of the latest seems to be severe, often presenting with orthostatic intolerance and fatigue symptoms, requiring expert consultation irrespective of the infection stage, and in case of dengue fever, frequently during the recovery phase. Meanwhile, in Covid-19 infected patients, the bradycardia observed is mild, frugal, and usually asymptomatic.
Thus, we conducted a comparison between two different groups of patients with viral infection displaying clinical bradycardia during hospital stay: Covid and non-Covid patients regarding clinical and Holter monitoring parameters. All patients had other causes of bradycardia excluded and echocardiography and cardiac biomarkers ruled out acute myocarditis.
The results showed that non-Covid patients presented with significantly lower mean and minimum heart rates (HR) on Holter monitoring, as well as longer times in with HR < 50 beats per minute (bpm). SDNN and pNN>50% were also significantly higher in non-Covid patients. The minimum systolic BP was significantly lower in non-Covid patients.
The study shows that Covid-19 is not the only viral infection that may display with clinical bradycardia, but it’s much milder than other viral infections such as dengue fever and viral diarrhea. It remains unclear the mechanism throughout Covid-related bradycardia comes about.
Notes on Fissidens flavolimbatus besch. and F. ghanae Bizot
Authors: Bruggeman-Nannenga Maria Alida
Published: June 2023

Fissidens flavolimbatus is discussed, and reduced to a synonym of F. madecassus, and F. ghanae is reinstated, described and figured. Fissidens madecassus, F. flavolimbatus and F. ghanae are lectotypified.
Revision of bryophyte specimens from Montenegro
Authors: Papp Beáta , Erzberger Peter , Dragicevic Snezana
Published: June 2023

More than hundred specimens of problematic or taxonomically re-evaluated taxa were revised in the Bryophyte Herbarium of the Hungarian Natural History Museum, Budapest (BP) and in the herbarium of P. Erzberger, Botanic Garden and Botanical Museum Berlin Dahlem (B). As result two species (Lophozia silvicola, Ulota interm edia) can be added to the Montenegrin bryophyte flora and one species (Brachythecium tenuicale) should be deleted. The recent distribution of several taxa of conservation interest was clarified, e. g. rarities like Neckera pumila, Ulota bruchii or genera potentially threatened due to climate change like Philonotis and Timmia. The results are important contributions to the preparation of an updated checklist and red list of the Montenegrin bryoflora.
Checklist and distribution of the liverworts of the Andasibe (Périnet) region (Madagascar)
Authors: Sass-Gyarmati Andrea , Hodgetts Nicholas G. , Brinda John C. , Abalo-Loko Goudjo-Ameto , Lavocat-Bernard Elisabeth , Vanderpoorten Alain , Boucheron-Dubuisson Elodie , Allard Amandine , Kervran Lionel , Andriamiarisoa Roger Lala , Reeb Catherine
Published: June 2023

This updated checklist of Marchantiophyta (liverworts) of Andasibé (Périnet) region, Madagascar was compiled from the literature, from herbarium specimens and recent collections. A total of 222 species including 9 infraspecific taxa, from 62 genera are recorded. Five species: Cheilolejeunea ngongensis Malombe et Pócs, Cheilolejeunea unciloba (Lindenb.) Malombe, Heteroscyphus grandistipus (Steph.) Schiffn., Lejeunea angulifolia Mitt. and Kymatocalyx africanus Vána et Wigginton are newly reported for Madagascar. Detailed informations on species occurrences are provided as a basis for subsequent research on species distributions and conservation.
Pócs’ rose (Rosa pocsii KER.-NAGY), distribution and new localities of an endemic rose species in Carpathian Basin
Authors: Kerényi-Nagy Viktor
Published: June 2023

In the current article I would like to present a little-known microspecies, the Pócs’ rose (Rosa pocsii Ker.-Nagy). In addition to the morphological description of the Pócs’ rose, I describe the distribution of the species based on the literature data and after the revision of herbarium collection I publish ten new localities for endemic Pócs’ rose.
TRAUMATIC ABDOMINAL AORTIC DISSECTION, A RARE NEAR-MISSED DIAGNOSIS: A CASE REPORT AND LITERATURE REVIEW
Authors:   Anesthesiology, Intensive Care and Emergency Department, University Hospital Mohammed VI of Marrakech, Morocco., Rahmani Chadi ,   Faculty of Medicine and Pharmacy of Marrakech, Cadi Ayyad University, Morocco., Kherchttou Yassine ,   Anesthesiology, Intensive Care and Emergency Department, University Hospital Mohammed VI of Marrakech, Morocco.,   Faculty of Medicine and Pharmacy of Marrakech, Cadi Ayyad University, Morocco., Rhezali Manal ,   Anesthesiology, Intensive Care and Emergency Department, University Hospital Mohammed VI of Marrakech, Morocco.,   Faculty of Medicine and Pharmacy of Marrakech, Cadi Ayyad University, Morocco., Abou Elhassan Taoufik ,   Anesthesiology, Intensive Care and Emergency Department, University Hospital Mohammed VI of Marrakech, Morocco.,   Faculty of Medicine and Pharmacy of Marrakech, Cadi Ayyad University, Morocco., Nejmi Hicham ,   Anesthesiology, Intensive Care and Emergency Department, University Hospital Mohammed VI of Marrakech, Morocco.,   Faculty of Medicine and Pharmacy of Marrakech, Cadi Ayyad University, Morocco.
Published: June 2023

Acute traumatic dissection of the abdominal aorta due to blunt trauma is a rare and life-threatening condition, often resulting in death. Immediate diagnosis is critical for successful treatment and improved prognosis. In this case presentation, a 60-year-old man was found unconscious after a road accident and was diagnosed with aortic dissection that went unnoticed initially due to associated severe injuries. CT-scan and ultrasound examination confirmed the diagnosis of abdominal aortic dissection. This case highlights the importance of a high index of suspicion when certain signs are present, such as seat-belt sign, bowel injury, or spinal injury. CT-scan is an accurate diagnostic tool, and close imaging follow-up is necessary as delayed complications can occur. Nonoperative and endovascular management is often chosen.
A STUDY TO ASSESS THE LEVEL OF SIGNIFICANCE OF COMPASSION FOR STAFF NURSES AT SMVMCH PUDUCHERRY
Authors:   M.Sc., (Nursing)-I year Sri ManakulaVinayagar Nursing College., R. Ramya , Manohari K. ,   Reader in nursing, Sri ManakulaVinayagar Nursing College., Muthamilselvi G. ,   Principal, Sri ManakulaVinayagar Nursing College.
Published: June 2023

Compassion is one of the key concepts of being a nurse. It is defined as asympathetic consciousness of others distress, together with a desire to alleviate it.Nurseswhoarecompassionatearehoweverexposedtotheriskofdevelopingcompassion fatigue, which has been linked to poor quality of care among nurses,increased staff turnover rate, decreased productivity, and low quality of life, all ofwhich led to a negative impact on patients safety and satisfaction, as well as on thenursesthemselves.
FINANCIAL INCLUSION- A FRONT WHEEL FOR WOMENS EMPOWERMENT
Authors: Kumar Singh Prashant , Singh Kirti
Published: June 2023

This study examines the impact of financial inclusion on womens empowerment in Uttar Pradesh, Madhya Pradesh, and Rajasthan, India, from a social, economic, political, legal, and psychological standpoint. An analysis of descriptive statistics revealed that financial inclusion and empowerment are significantly correlated among diverse women. Based on the regression analysis results, financial inclusion is crucial to womens empowerment in general. Several factors contribute to womens empowerment in these areas, including financial literacy initiatives and access to banking services. Furthermore, the paper stresses the need for a targeted strategy and program to promote financial inclusion for women, gender equality, and inclusive growth in Uttar Pradesh, Madhya Pradesh, and Rajasthan.
PRODUCTION AND OPTIMIZATION OF BIOPLASTIC FROM BACILLUS ISOLATED FROM ENVIRONMENTAL SAMPLE
Authors:   Department of Biosciences, Mangalore University, Mangalagangothri, Karnataka-574199., Sheri John Britto Jolitta , Priyakumar Austin ,   Athmic Biotech Solutions Pvt Ltd R&D Lab, Kaliyoor - Punnamoodu Road, Kalliyoor, Kerala- 695042, India., R Nair Nimisha ,   Athmic Biotech Solutions Pvt Ltd R&D Lab, Kaliyoor - Punnamoodu Road, Kalliyoor, Kerala- 695042, India.
Published: June 2023

Biopolymers represent a major alternative to petroleum-derived plastics, with poly-3-hydroxybutyrate (PHB) being the most common. To reduce the cost of production and expand the use of such biopolymers, our research focused on identifying microorganisms with effective PHB-producing capabilities. Through standard microbiological procedures, we isolated six different samples and used Sudan Black B colony staining to determine their PHB-producing capabilities. The Crotonic acid method revealed a 90.1% yield of PHB in the sample. Biochemical, morphological, and molecular analyses - including 16S rRNA gene sequencing - confirmed the bacterium as Bacillus cereus. FTIR analysis was also used to confirm the polymer as PHB. Furthermore, the ability of Bacillus cereus to use various carbon sources, coupled with gentamycin encapsulated in PHB polymer sheets to effectively demonstrate its antimicrobial properties, and a cell viability analysis to show its biocompatibility, highlight this microorganism as an effective candidate for large-scale industrial production of bacterial polyhydroxybutyrate.
THE RELATIONSHIP BETWEEN FAMILY ATTACHMENT AND DEVIANT BEHAVIORS OF HIGH SCHOOL STUDENTS
Authors:   University of Education - VNU., Thi Mai Phuong Tran
Published: June 2023

The study aimed to assess the relationship between the level of family attachment and the deviant behaviors of high school students - survey data from 126 high school students of Hanoi city. Research results show that: family cohesion is negatively correlated with the bad behavior of high school students. In families where parents often criticize and impose on their children, family members often have conflicts and conflicts, and the childrens morale is higher. In families where family members often have inappropriate behaviors and words, children also have high expressions of similar deviant behaviors.
ASSESSMENT OF BODY FAT COMPOSITION PARAMETERS IN WOMEN OF POLYCYSTIC OVARY SYNDROME (PCOS) : A CASE- CONTROL STUDY
Authors:   Junior Resident., Agarwal Shweta , Rathi Preeti ,   Professor and Head, Department of Physiology, S.M.M.H Medical College, Sharanpur., Lalita Chaudhary ,   Professor and Head, Department of Physiology, LLRM Medical College, Meerut., Tandon Anshu ,   Associate Professor, Department of Physiology, LLRM Medical College, Meerut., Kulshreshtha Megha ,   Assistant Professor, Department of Physiology, LLRM Medical College, Meerut., Verma Aruna ,   Professor, Department of Obstetrics and Gynaecology, LLRM Medical College, Meerut.
Published: June 2023

Introduction: Polycystic ovary syndrome is a complex condition of women characterized by menstrual irregularities, elevated androgen levels & polycystic ovarian morphology. It is often associated with psychological impairment , metabolic derangements and obesity . Objectives: Present study has been done to assess body fat mass composition in PCOS patients & to compare it with age-matched controls. Materials and Methods ÃƒÂ‚Ã‚Â– 29 newly diagnosed PCOS patients as per Rotterdam criterion were recruited from the Obstetrics and Gynaecology OPD of SVBP hospital, Meerut. The cases were compared with 30 healthy age-matched women which acted as controls. Bioelectrical Impedance Analysis was used to record Body composition parameters such as Total body water, Soft Lean Mass, Fat Free Mass, Skeletal Muscle Mass, Body Fat Mass, Percentage Body Fat, Visceral Fat level, Body Mass Index, Fat-Free Mass Index, Skeletal Muscle Index. Anthropometric parameters including weight and height were also recorded. Results: Body Mass Index (BMI), Skeletal Mass Index (SMI), Fat-Free Mass Index (FFMI) were significantly higher in patients with PCOS (p<0.01). Further, as compared to control group, other body fat composition parameters were also higher in patients of PCOS. Conclusion: This study exhibits that women with PCOS have higher Body Fat and Skeletal Muscle parameters as compared to age-matched controls. Annual Screening of patients with PCOS by performing Body Composition Analysis will help them to maintain their body fat and muscle ratio in defined values & also prevent the various diseases associated with higher body fat in PCOS.
Polymer-Supported Dibutylstannyl Azide: An Efficient and Recoverable Reagent in the One-Pot Synthesis of Aryl Azides and 5-Aryl 1H-tetrazoles
Authors: Godoy Prieto Leonela , Lockhart María , Lo Fiego Marcos
Published: June 2023

Herein, a very stable and highly reactive insoluble polymer-supported organotin azide was prepared from a Merrifield resin and its azide loading was determined by elemental analysis. This immobilized azide was employed in one-pot diazotization-azidodediazoniation of aromatic amines to provide a wide range of aryl azides in very good yields under mild conditions. On the other hand, this supported reagent was successfully applied in the 1,3-dipolar cycloaddition reaction with aromatic nitriles to provide 5-aryl 1H-tetrazoles in good yields. In addition, the recyclability of tin azide from the supported dibutyltin sulfonate recovered in the synthesis processes was reported. The developed methods allow the recycling and reuse of the supported tin azide, without significant loss of reactivity after four cycles in our reaction scale and less than 20 ppm of residual tin concentration in final products without additional purification.
Baskı Tasarımı ve Nakış Uygulamasında Fosforlu İplik Kullanımı
Authors: Bilir Mehmet Zahit
Published: June 2023

Günümüz rekabet koşullarında yenilikçi ürünlerin üretilmesi, ülkelerin ticaret güçlerinin arttırılması açısından giderek artan bir öneme sahiptir. Tekstil sektöründe kullanılan yenilikçi ürünlerden biriside fosforlu ipliklerdir. Fosforlu iplikler, güneşten veya başka bir ışık kaynağından gelen ışık enerjisini absorbe edebilen, bu enerjiyi yapısında tutabilen ve daha sonra yapısındaki ışık enerjisini karanlık ortamda tekrar yayabilen iplik çeşitlerindendir. Tekstilde fosforlu malzemeler ile direkt baskı, dokuma, nakış işleme işleri vb. yapılarak çeşitli tekstil ürünleri oluşturulabilmektedir. Bu çalışmada ise fosforlu iplikler dokuma yüzeye özel olarak işlenmekte ve işlenen yüzey üzerinde yapılacak tasarım alanı dışında kalan fosforlu alanlar ise ışık almayacak şekilde baskı işlemi ile kapatılmaktadır. Bu yönü ile yapılan uygulamanın diğer tasarım yöntemlerine göre farklılığı da gösterilmektedir. Çalışma sonucunda, fosforlu ipliklerin tekstil sektöründe kullanımına yönelik farkındalığın arttırılması ve fosforlu iplik işlemesi ile tekstil baskı işleminin beraber kullanılmasıyla, farklı ve ilgi çeken tasarımların yapılabileceği gösterilmektedir.