bluuebunny/crossref_metadata_2025 huggingface dataset:
Analysis of the Association Between MicroRNA Biogenesis Gene Polymorphisms and Venous Thromboembolism in Koreans
Authors: Ko Eun Ju , Kim Eo Jin , Kim Jung Oh , Sung Jung Hoon , Park Han Sung , Ryu Chang Soo , Oh Jisu , Chong So Young , Oh Doyeun , Kim Nam Keun
Published: August 2019

Venous thromboembolism (VTE) involves the formation of a blood clot, typically in the deep veins of the leg or arm (deep vein thrombosis), which then travels via the circulatory system and ultimately lodges in the lungs, resulting in pulmonary embolism. A number of microRNAs (miRNAs) are well-known regulators of thrombosis and thrombolysis, and mutations in miRNA biogenesis genes, such as DICER1, DROSHA have been implicated in miRNA synthesis and function. We investigated the genetic association between polymorphisms in four miRNA biogenesis genes, DICER1 rs3742330A > G, DROSHA rs10719T > C, RAN rs14035C > T and XPO5 rs11077A > C, and VTE in 503 Koreans: 300 controls and 203 patients. Genotyping was assessed with polymerase chain reaction-restriction fragment length polymorphism assays. We detected associations between polymorphisms in RAN and XPO5 and VTE prevalence (RAN rs14035CC + CT versus TT: p = 0.018; XPO5 rs11077AA + AC versus CC: p < 0.001). Analysis of allele combinations of all four polymorphisms (DICER1, DROSHA, RAN, XPO5) revealed that A-T-T-A was associated with decreased VTE prevalence (p = 0.0002), and A-T-C-C was associated with increased VTE prevalence (p = 0.027). Moreover, in subjects with provoked VTE, the DROSHA rs10719T > C, polymorphism was associated with increased disease prevalence (TT versus TC + CC: p < 0.039). Our study demonstrates that RAN and XPO5 polymorphisms are associated with risk for VTE in Korean subjects.
Aedes aegypti Males as Vehicles for Insecticide Delivery
Authors: Brelsfoard Corey L. , Mains James W. , Mulligan Steve , Cornel Anthony , Holeman Jodi , Kluh Susanne , Leal Andrea , Hribar Lawrence J. , Morales Harold , Posey Tanya , Dobson Stephen L.
Published: August 2019

Aedes aegypti continues to spread globally and remains a challenge to control, in part due to its ‘cryptic behavior’ in that it often deposits eggs (oviposits) in larval habitats that are difficult to find and treat using traditional methods. Auto-dissemination strategies target these cryptic breeding sites by employing mosquitoes to deliver lethal doses of insecticide. This report describes the initial field trials of an application known as Autodissemination Augmented by Males (ADAM), utilizing A. aegypti males dusted with pyriproxyfen (PPF). Findings presented here are drawn from both caged and field trial studies. Together, these trials examined for the ability of A. aegypti males to disseminate PPF and to impact field populations. PPF-dusted males were able to effectively deliver lethal doses of PPF to oviposition sites under the conditions tested. Results from field trials in Florida and California demonstrated reduced A. aegypti populations in treated areas, compared to areas where PPF-treated males were not released. These results indicate that the release of PPF-dusted A. aegypti males can impact A. aegypti populations as measured by both reduced larval survival and lower numbers of adult female A. aegypti. We propose the ADAM approach as an addition to existing mosquito control techniques targeting A. aegypti and other mosquitoes that utilize cryptic larval habitats.
Congruence Between Molecular Data and Morphology: Phylogenetic Position of Senodoniini (Coleoptera: Elateridae)
Authors: Kundrata Robin , Prosvirov Alexander S. , Vondracek Dominik , Sormova Eliska
Published: August 2019

Senodoniini is a small lineage of click beetles currently comprising 21 species in two genera, distributed in the Himalayas and East and Southeast Asia. The definition and limits of this group have changed considerably during its history. Recent authors treat Senodoniini as a tribe within Dendrometrinae, usually close to Dimini, but this placement has never been rigorously tested. Here, we shed new light on the systematic position and limits of Senodoniini by performing a combined phylogenetic analysis of two nuclear and two mitochondrial molecular markers. Our results recovered Senodoniini not monophyletic, and placed them into the Lissominae complex, where they formed a clade with Austrelater Calder & Lawrence (Protelaterini). Molecular phylogeny is in agreement with the adult morphology. Additionally, we examined the morphology of a monotypic genus Rostricephalus Fleutiaux from Southeast Asia, which was previously classified in various Elateridae groups including Senodoniini, and its position was always uncertain. This genus shares morphological characters with Protelaterini. We provide morphological redescriptions as well as the figures of main diagnostic characters for Senodonia Laporte, Sossor Candèze, and Rostricephalus. Based on our results, we place these genera to Lissominae: Protelaterini, and hence synonymize Senodoniini Schenkling with Protelaterini Schwarz.
Drive for Thinness Predicts Musculoskeletal Injuries in Division II NCAA Female Athletes
Authors: Scheid Jennifer L. , Stefanik Morgan E.
Published: August 2019

The female athlete triad is the interrelation of low energy availability, menstrual dysfunction, and low bone mineral density. Previously, the components of the female athlete triad have been linked to bone stress injuries. The objective of this study was to explore the relationship between drive for thinness, a proxy indicator of low energy availability, and musculoskeletal injuries. Fifty-seven female athletes, from an NCAA Division II college, were followed throughout their respective sport season for musculoskeletal injuries. Women were grouped based on a median split of the drive for thinness score (high drive for thinness (DT) vs. low DT). At the end of each sport season, injury data were compiled using an electronic medical record database. Forty-seven of the 57 women (82%) incurred 90 musculoskeletal injuries. The most prevalent injuries included: Low back pain/spasm/strain (n = 12), followed by shin splints/medial tibial stress syndrome (n = 9), general knee pain (n = 7), quadriceps strain (n = 6), and knee sprain (anterior cruciate ligament, posterior cruciate ligament, medial collateral ligament, and lateral collateral ligament sprains; n = 5). The number of in-season injuries in the High DT group (2.0 ± 0.3) was significantly higher than the Low DT group (1.2 ± 0.2, p = 0.026). A high drive for thinness is associated with an increased number of injuries during the competitive season.
Multi-Scale Structural Assessment of Cellulose Fibres Cement Boards Subjected to High Temperature Treatment
Authors: Gorzelańczyk Tomasz , Pachnicz Michał , Różański Adrian , Schabowicz Krzysztof
Published: August 2019

The methodology of multi-scale structural assessment of the different cellulose fibre cement boards subjected to high temperature treatment was proposed. Two specimens were investigated: Board A (air-dry reference specimen) and Board B (exposed to a temperature of 230 °C for 3 h). At macroscale all considered samples were subjected to the three-point bending test. Next, two methodologically different microscopic techniques were used to identify evolution (caused by temperature treatment) of geometrical and mechanical morphology of boards. For that purpose, SEM imaging with EDS analysis and nanoindentation tests were utilized. High temperature was found to have a degrading effect on the fibres contained in the boards. Most of the fibres in the board were burnt-out, or melted into the matrix, leaving cavities and grooves which were visible in all of the tested boards. Nanoindentation tests revealed significant changes of mechanical properties caused by high temperature treatment: “global” decrease of the stiffness (characterized by nanoindentation modulus) and “local” decrease of hardness. The results observed at microscale are in a very good agreement with macroscale behaviour of considered composite. It was shown that it is not sufficient to determine the degree of degradation of fibre-cement boards solely on the basis of bending strength; advanced, microscale laboratory techniques can reveal intrinsic structural changes.
Effect of Addition of Ca2+ and CO32− Ions with Temperature Control on Self-Healing of Hardened Cement Paste
Authors: Choi Heesup , Inoue Masumi , Kim Dongmin , Choi Hyeonggil , Sengoku Risa
Published: August 2019

Concrete has a remarkably low ratio of tensile strength to compressive strength, and is widely used in construction. However, the occurrence of cracks in a concrete structure is inevitable. Nevertheless, in the presence of adequate moisture, small cracks in the concrete structure exhibit a propensity to self-heal by getting filled due to the rehydration of cement particles and the subsequent precipitation of calcium carbonate (CaCO3). According to previous studies, the self-healing performance can be maximized by optimizing the temperature and pH to control the crystal formation of CaCO3. This study focused on the crystal form of CaCO3 generated in the self-healing of a cement-based composite material. To evaluate the self-healing performance depending on the type of aqueous solution and the temperature, the weight change, the weight change rate, and the porosity reduction in each case were evaluated. Moreover, to increase the generation of CaCO3 (which is a self-healing precipitate), nanosized ultrafine CO2 bubbles using CO2 gas were used, along with an adequate supply of Ca2+ by adjusting the aqueous solution (Ca(OH)2, CaO + ethanol). For greater pore-filling effects by controlling the CaCO3 crystal forms in the cement matrix, the change in the crystal form of the precipitated CaCO3 in the hardened cement paste with changing temperature was analyzed by scanning electron microscopy and X-ray diffraction. As a result, the possibility of the effective generation and control of vaterite with a dense pore structure together with calcite was confirmed by adjusting the temperature to approximately 40 °C at a pH of 12.
Identification of Antimicrobial Peptides from the Microalgae Tetraselmis suecica (Kylin) Butcher and Bactericidal Activity Improvement
Authors: Guzmán Fanny , Wong Genezareth , Román Tanya , Cárdenas Constanza , Alvárez Claudio , Schmitt Paulina , Albericio Fernando , Rojas Verónica
Published: August 2019

The outburst of microbial resistance to antibiotics creates the need for new sources of active compounds for the treatment of pathogenic microorganisms. Marine microalgae are of particular interest in this context because they have developed tolerance and defense strategies to resist the exposure to pathogenic bacteria, viruses, and fungi in the aquatic environment. Although antimicrobial activities have been reported for some microalgae, natural algal bioactive peptides have not been described yet. In this work, acid extracts from the microalga Tetraselmis suecica with antibacterial activity were analyzed, and de novo sequences of peptides were determined. Synthetic peptides and their alanine and lysine analogs allowed identifying key residues and increasing their antibacterial activity. Additionally, it was determined that the localization of positive charges within the peptide sequence influences the secondary structure with tendency to form an alpha helical structure.
Long-Chain Bases from Sea Cucumber Alleviate Obesity by Modulating Gut Microbiota
Authors: Hu Shiwei , Xu Yangli , Gao Xiang , Li Shijie , Jiang Wei , Liu Yu , Su Laijin , Yang Huicheng
Published: August 2019

This study evaluated the effects of long-chain bases from sea cucumber (SC-LCBs) on modulation of the gut microbiota and inhibition of obesity in high fat diet-fed mice. Results showed that SC-LCBs exerted significant antiobese effects, which were associated with the inhibition of hyperglycemia and lipid accumulation. SC-LCBs also regulated serum adipocytokines toward to normal levels. SC-LCBs caused significant decreases in Firmicutes, Actinobacteria phylum, and obesity-related bacteria (Desulfovibro, Bifidobacterium, Romboutsia etc. genus). SC-LCBs also elevated Bacteroidetes, Proteobacteria, Verrucomicrobia phylum, and short chain fatty acids (SCFAs)-producing bacteria (Bacteroides, Lactobacillus, Lachnospiraceae_NK4A136_group etc. genus). Moreover, serum and fecal lipoplysaccharide (LPS) concentrations and its dependent toll-line receptor 4 pathway were inhibited by SC-LCBs treatment. SC-LCBs caused increases in fecal SCFAs and their mediated G-protein-coupled receptors proteins. These suggest that SC-LCBs alleviate obesity by altering gut microbiota. Thus, it sought to indicate that SC-LCBs can be developed as food supplement for the obesity control and the human gut health.
Evaluation of Clinical and Laboratory Characteristics of Children with Pulmonary and Extrapulmonary Tuberculosis
Authors: Aygun Deniz , Akcakaya Necla , Cokugras Haluk , Camcıoglu Yıldız
Published: August 2019

Background and objective: Tuberculosis (TB) is an important public health problem in both developing and developed countries. Childhood TB is also an important epidemiological indicator in terms of forming the future TB pool. The diagnosis of TB is difficult in children due to the lack of a standard clinical and radiological description. We aimed to evaluate and compare the clinical, laboratory, and radiologic findings of childhood pulmonary and extrapulmonary TB. Material and Methods: The medical records of patients hospitalized with the diagnosis of pulmonary tuberculosis (PTB) and extrapulmonary tuberculosis (EPTB) between December 2007 and December 2017 were evaluated retrospectively. Results: There were 163 patients diagnosed with TB with 94 females (57.7%) and 69 males (42.3%). Seventy-three patients (44.8%) had PTB, 71 (43.6%) patients had EPTB, and 19 patients (11.7%) had both PTB and EPTB, called as disseminated TB. Ninety-six (58.9%) patients had tuberculin skin test (TST) positivity and 64 patients (39.3%) had interferon-gamma release assay (IGRA) positivity. Acid-resistant bacteria were observed in 34 (20.9%) body fluid samples and culture positivity was observed in 33 (20.2%) samples. Comparison of PTB, EPTB, and disseminated TB revealed that low socioeconomic status, TB contact, and low body weight were more common in disseminated TB, and TST positivity was more common in PTB. Conclusion: Malnutrition, low socioeconomic status, and TB contact were important diagnostic variables in our study and all three parameters were more common in disseminated TB. Tuberculosis should be considered in patients admitted with different complaints and signs in populations with high TB incidence and low socioeconomic status.
Development of PVDF Ultrafiltration Membrane with Zwitterionic Block Copolymer Micelles as a Selective Layer
Authors: Thankappan Hajeeth , Bousquet Gauthier , Semsarilar Mona , Venault Antoine , Chang Yung , Bouyer Denis , Quemener Damien
Published: August 2019

In recent years, block copolymer micellar assemblies with the formation of structured nanoparticles have been considered as an emerging technology in membrane science. In this work, the poly(methyl methacrylate)-block-poly(sulfobetaine methacrylate) copolymer was directly synthesized using Reversible Addition-Fragmentation chain Transfer (RAFT) polymerization and self-assembled in a selective medium (2,2,2-trifluroethanol/water). Then, poly(methyl methacrylate)-block-poly(sulfobetaine methacrylate) copolymers were casted onto a commercial PVDF membrane to form a thin porous selective layer. The prepared nanoparticles and the resulting membranes were fully characterized using microscopy methods (SEM and AFM), whereas the membrane performance was evaluated in terms of permeability and the molecular weight cut off. The results from this study demonstrate the preparation of an ultrafiltration membrane made from the assembly of poly(methyl methacrylate)-block-poly(sulfobetaine methacrylate) copolymer micelles on the top of a PVDF membrane in the form of thin film. The copolymer chain orientation leads to a membrane surface enriched in hydrophilic PSBMA, which confers a suitable behavior for aqueous solution filtration on the membrane, while preserving the high chemical and mechanical resistance of the PVDF.
Synthesis, Characterization, and Pesticidal Activity of Emamectin Benzoate Nanoformulations against Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae)
Authors: Elabasy Asem , Shoaib Ali , Waqas Muhammad , Jiang Mingxing , Shi Zuhua
Published: August 2019

Using nanotechnology to develop new formulations of pesticides is considered a possible option in enhancing the efficiency, safety, and photostability of pesticides under various climatic conditions. In the present study, two novel nanoformulations (NFs) were successfully prepared based on nano-delivery systems for emamectin benzoate (EMB) by loading it on cellulose nanocrystals (CNCs) and silicon dioxide nanoparticles (SNPs) as carriers through a freeze-drying method. The synthesized nanoformulations were examined using field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and dynamic light scattering (DLS). The results showed that SNPs and CNCs had a loading efficiency of 43.31% and 15.04% (w/w) for EMB, respectively, and could effectively protect EMB from photolysis under UV radiation. The LC50 values for EMB + SNPs, EMB + CNCs, and EMB commercial formulation against Phenacoccus solenopsis were 0.01, 0.05, and 0.31 μg/mL, respectively, indicating that both NFs were more effective than the EMB commercial formulation. This work seeks to develop new nano-carriers for potential applications of pesticides in plant protection, which will reduce the recommended dose of pesticides and thereby decrease the amount of pesticide residue in food and the environment.
Mammalian DNA Polymerase Kappa Activity and Specificity
Authors: Stern Hannah R. , Sefcikova Jana , Chaparro Victoria E. , Beuning Penny J.
Published: August 2019

DNA polymerase (pol) kappa is a Y-family translesion DNA polymerase conserved throughout all domains of life. Pol kappa is special6 ized for the ability to copy DNA containing minor groove DNA adducts, especially N2-dG adducts, as well as to extend primer termini containing DNA damage or mismatched base pairs. Pol kappa generally cannot copy DNA containing major groove modifications or UV-induced photoproducts. Pol kappa can also copy structured or non-B-form DNA, such as microsatellite DNA, common fragile sites, and DNA containing G quadruplexes. Thus, pol kappa has roles both in maintaining and compromising genomic integrity. The expression of pol kappa is altered in several different cancer types, which can lead to genome instability. In addition, many cancer-associated single-nucleotide polymorphisms have been reported in the POLK gene, some of which are associated with poor survival and altered chemotherapy response. Because of this, identifying inhibitors of pol kappa is an active area of research. This review will address these activities of pol kappa, with a focus on lesion bypass and cellular mutagenesis.
Pharmacophoric Site Identification and Inhibitor Design for Autotaxin
Authors: Lee Myeong Hwi , Lee Dae-Yon , Balupuri Anand , Jeong Jong-Woo , Kang Nam Sook
Published: August 2019

Autotaxin (ATX) is a potential drug target that is associated with inflammatory diseases and various cancers. In our previous studies, we have designed several inhibitors targeting ATX using computational and experimental approaches. Here, we have analyzed topological water networks (TWNs) in the binding pocket of ATX. TWN analysis revealed a pharmacophoric site inside the pocket. We designed and synthesized compounds considering the identified pharmacophoric site. Furthermore, we performed biological experiments to determine their ATX inhibitory activities. High potency of the designed compounds supports the predictions of the TWN analysis.
Impact of Different Storage Methods on Bioactive Compounds in Arthrospira platensis Biomass
Authors: Papalia Teresa , Sidari Rossana , Panuccio Maria Rosaria
Published: August 2019

Arthrospira platensis (spirulina) is considered a source of natural molecules with nutritional and health benefits. As the different storage forms can affect the quantity and quality of bioactive ingredients, the aim of the present work was to evaluate the effects of freezing, oven-drying and freeze-drying on chemical composition of spirulina biomass. Total proteins, photosynthetic pigments and antioxidants, were analyzed and compared to respective quantities in fresh biomass. The frozen sample exhibited the highest content of phycocyanin-C, phenols, and ascorbic acid, also respect to the fresh biomass. The highest total flavonoid amount was in the freeze-dried biomass. HPLC-DAD analysis of phenolic acids revealed the presence of the isoflavone genistein, known for its therapeutic role, in all the spirulina samples. The phosphomolybdenum method (TAC) and DPPH scavenging activity were applied to determine the antioxidant activity of different samples. The highest DPPH scavenging activity was detected in fresh and freeze-dried biomass and it was positively related to carotenoid content. A positive correlation indicated that carotenoids, chlorophyll, ascorbic acid and all phenolic compounds were the major contributors to the TAC activity in spirulina biomass. The results highlighted a different functional value of spirulina biomass, depending on the processing methods used for its storage.
Surface Effects and Optical Properties of Self-Assembled Nanostructured a-Si:Al
Authors: Kjeldstad Torunn , Thøgersen Annett , Stange Marit , Jensen Ingvild Thue , Monakhov Eduard , Galeckas Augustinas
Published: August 2019

We present a study of the surface effects and optical properties of the self-assembled nanostructures comprised of vertically aligned 5 nm-diameter Al nanowires embedded in an amorphous Si matrix (a-Si:Al). The controlled (partial) removal of Al nanowires in a selective etching process yielded nanoporous a-Si media with a variable effective surface area. Different spectroscopy techniques, such as X-ray photoelectron spectroscopy (XPS), UV-Vis spectrophotometry and photoluminescence (PL), have been combined to investigate the impact of such nanostructuring on optical absorption and emission properties. We also examine long-term exposure to air ambient and show that increasing level of surface oxidation determines the oxide defect-related nature of the dominant PL emission from the nanoporous structures. The role of bulk, nanosize and surface effects in optical properties has been separated and quantified, providing a better understanding of the potential of such nanoporous a-Si:Al structures for future device developments.
Influence of Vitamin C on Antioxidant Capacity of In Vitro Perfused Porcine Kidneys
Authors: Bleilevens Christian , Doorschodt Benedict M. , Fechter Tamara , Grzanna Tim , Theißen Alexander , Liehn Elisa A. , Breuer Thomas , Tolba René H. , Rossaint Rolf , Stoppe Christian , Boor Peter , Hill Aileen , Fabry Gregor
Published: August 2019

Systemic and localized ischemia and reperfusion injury remain clinically relevant issues after organ transplantation and contribute to organ dysfunctions, among which acute kidney injury is one of the most common. An in vitro test-circuit for normothermic perfusion of porcine kidneys after warm ischemia was used to investigate the antioxidant properties of vitamin C during reperfusion. Vitamin C is known to enhance microcirculation, reduce endothelial permeability, prevent apoptosis, and reduce inflammatory reactions. Based on current evidence about the pleiotropic effects of vitamin C, we hypothesize that the antioxidant properties of vitamin C might provide organ-protection and improve the kidney graft function in this model of ischemia and reperfusion. Methods: 10 porcine kidneys from 5 Landrace pigs were perfused in vitro for 6 h. For each experiment, both kidneys of one animal were perfused simultaneously with a 1:1 mixture of autologous blood and modified Ringer’s solution at 38 °C and 75 mmHg continuous perfusion pressure. One kidney was treated with a 500 mg bolus injection of vitamin C into the perfusate, followed by continuous infusion of 60 mg/h vitamin C. In the control test circuit, an equal volume of Ringer’s solution was administered as a placebo. Perfusate samples were withdrawn at distinct points in time during 6 h of perfusion for blood gas analyses as well as measurement of serum chemistry, oxidative stress and antioxidant capacity. Hemodynamic parameters and urine excretion were monitored continuously. Histological samples were analyzed to detect tubular- and glomerular-injury. Results: vitamin C administration to the perfusate significantly reduced oxidative stress (49.8 ± 16.2 vs. 118.6 ± 23.1 mV; p = 0.002) after 6 h perfusion, and increased the antioxidant capacity, leading to red blood cell protection and increased hemoglobin concentrations (5.1 ± 0.2 vs. 3.9 ± 0.6 g/dL; p = 0.02) in contrast to placebo treatment. Kidney function was not different between the groups (creatinine clearance vit C: 2.5 ± 2.1 vs. placebo: 0.5 ± 0.2 mL/min/100 g; p = 0.9). Hypernatremia (187.8 ± 4.7 vs. 176.4 ± 5.7 mmol/L; p = 0.03), and a lower, but not significant decreased fractional sodium excretion (7.9 ± 2 vs. 27.7 ± 15.3%; p = 0.2) were observed in the vitamin C group. Histological analysis did not show differences in tubular- and glomerular injury between the groups. Conclusion: Vitamin C treatment increased the antioxidant capacity of in vitro perfused kidney grafts, reduced oxidative stress, preserved red blood cells as oxygen carrier in the perfusate, but did not improve clinically relevant parameters like kidney function or attenuate kidney damage. Nevertheless, due to its antioxidative properties vitamin C might be a beneficial supplement to clinical kidney graft perfusion protocols.
Gender Differences in Phytoestrogens and the Relationship with Speed of Processing in Older Adults: A Cross-Sectional Analysis of NHANES, 1999–2002
Authors: Alwerdt Jessie , Patterson Andrew D. , Sliwinski Martin J.
Published: August 2019

Sex hormone changes in adults are known to play a part in aging, including cognitive aging. Dietary intake of phytoestrogens can mimic estrogenic effects on brain function. Since sex hormones differ between genders, it is important to examine gender differences in the phytoestrogen–cognition association. Therefore, the goal of this study is to examine the relationship between urinary phytoestrogens and speed of processing (SOP) and the variation of the association between genders in older adults. Participants were drawn from the 1999–2002 National Health and Nutrition Examination Survey and included 354 individuals aged 65–85 years old. General linear models (GLMs) were used to test for significant gender differences in the relationship between phytoestrogens and SOP. Results from the GLMs showed significant gender differences in the relationship between genistein and SOP. Higher levels of genistein were associated with better SOP in women. This relationship was reversed in men: higher genistein levels were associated with worse performance. Results indicate that there are distinct gender differences in the relationship between genistein and SOP. These results emphasize the importance of considering gender differences when devising dietary and pharmacologic interventions that target phytoestrogens to improve brain health.
Designing Fast-Dissolving Orodispersible Films of Amphotericin B for Oropharyngeal Candidiasis
Authors: Serrano Dolores R. , Fernandez-Garcia Raquel , Mele Marta , Healy Anne Marie , Lalatsa Aikaterini
Published: August 2019

Amphotericin B possesses high activity against Candida spp. with low risk of resistance. However, Amphotericin B’s high molecular weight compared to other antifungal drugs, such as miconazole and clotrimazole, and poor water solubility hampers its efficacy at the physiological conditions of the oropharyngeal cavity (saliva pH, limited volume for dissolution) and thereby limits its clinical use in oropharyngeal candidiasis. We have prepared fast-dissolving orodispersible films with high loading (1% w/w) using solvent casting that enables amphotericin B to remain solubilised in saliva in equilibrium between the monomeric and dimeric states, and able to produce a local antifungal effect. Optimisation of the amphotericin B-loaded orodispersible films was achieved by quality by design studies combining dextran and/or maltodextrin as dextrose-derived-polymer film formers with cellulose-derived film formers (hydroxypropylmethyl/hydroxypropyl cellulose in a 1:4 weight ratio), sorbitol for taste masking, microcrystalline cellulose (Avicel 200) or microcrystalline cellulose-carboxymethylcellulose sodium (Avicel CL-611) for enhancing the mechanical strength of the film, and polyethylene glycol 400 and glycerol (1:1 w/w) as plasticizers. The optimised amphotericin B orodispersible films (containing 1% AmB, 25% dextran, 25% maltodextrin, 5% sorbitol, 10% Avicel 200, 10% polyethylene glycol 400, 10% glycerol, 3% hydroxypropylmethyl cellulose acetate succinate, 12% hydroxypropyl cellulose) possessed a fast disintegration time (60 ± 3 s), quick release in artificial saliva (>80% in 10 min), high burst strength (2190 mN mm) and high efficacy against several Candida spp. (C. albicans, C. parapsilosis and C. krusei) (>15 mm inhibition halo). Amphotericin B orodispersible films are stable for two weeks at room temperature (25 °C) and up to 1 year in the fridge. Although further toxicological and in vivo efficacy studies are required, this novel Amphotericin B orodispersible films is a promising, physicochemically stable formulation with potential wide application in clinical practice, especially for immunocompromised patients suffering from oropharyngeal candidiasis.
Mucosal Vaccination via the Respiratory Tract
Authors: Hellfritzsch Marie , Scherließ Regina
Published: August 2019

Vaccine delivery via mucosal surfaces is an interesting alternative to parenteral vaccine administration, as it avoids the use of a needle and syringe. Mucosal vaccine administration also targets the mucosal immune system, which is the largest lymphoid tissue in the human body. The mucosal immune response involves systemic, antigen-specific humoral and cellular immune response in addition to a local response which is characterised by a predominantly cytotoxic T cell response in combination with secreted IgA. This antibody facilitates pathogen recognition and deletion prior to entrance into the body. Hence, administration via the respiratory mucosa can be favoured for all pathogens which use the respiratory tract as entry to the body, such as influenza and for all diseases directly affecting the respiratory tract such as pneumonia. Additionally, the different mucosal tissues of the human body are interconnected via the so-called “common mucosal immune system”, which allows induction of an antigen-specific immune response in distant mucosal sites. Finally, mucosal administration is also interesting in the area of therapeutic vaccination, in which a predominant cellular immune response is required, as this can efficiently be induced by this route of delivery. The review gives an introduction to respiratory vaccination, formulation approaches and application strategies.
Abramovsky—Gribov—Kancheli Theorem in the Physics of Black Holes
Authors: Abramovsky Victor A.
Published: August 2019

The proof of the Abramovsky—Gribov—Kancheli (AGK) theorem for black hole physics is given. Based on the AGK relations, a formula for the luminosity of a black hole as a function of the mass of the black hole is derived. The correspondence to experimental data is considered. It is shown that the black holes of the galaxies NGC3842 and NGC4889 do not differ from those of the other galaxies.
Blue Light-Initiated Alcoholic RAFT Dispersion Polymerization of Benzyl Methacrylate: A Detailed Study
Authors: Liu Dongdong , Zeng Ruiming , Sun Hao , Zhang Li , Tan Jianbo
Published: August 2019

Blue light-initiated alcoholic reversible addition-fragmentation chain transfer (RAFT) dispersion polymerization of benzyl methacrylate (BzMA) using bis (acyl) phosphane oxide (BAPO) as the photo-initiator is developed to prepare diblock copolymer nano-objects. High monomer conversion (95%) was achieved within 2 h of blue light irradiation in an isopropanol/water mixture. Effects of solvent, light intensity, and reaction temperature on the polymerization kinetics were evaluated. Finally, the effect of reaction temperature on the morphologies of diblock copolymer nano-objects was investigated and two morphological phase diagrams were constructed at 25 and 70 °C. Transmission electron microscopy (TEM) measurement confirmed that increasing the reaction temperature promoted the evolution of higher order morphology. We believe this study will provide more mechanistic insights into alcoholic RAFT dispersion polymerization for the creation of diblock copolymer nano-objects with well-defined structures.
Applying a Watershed and Reservoir Model in an Off-Site Reservoir to Establish an Effective Watershed Management Plan
Authors: Chen Chi-Feng , Wu Yi-Ru , Lin Jen-Yang
Published: August 2019

Off-site reservoirs use water from other watersheds to supplement their water quantity. Water quality is usually better in off-site reservoirs than in onsite reservoirs, because in comparison to onsite reservoirs, watershed areas are smaller and fewer pollutants are collected; moreover, cleaner water is introduced. However, in Taiwan, the water quality of some off-site reservoirs can gradually worsen, and this factor needs to be addressed. In this study, the Liyutan reservoir in central Taiwan was used as an example to demonstrate the process of evaluating pollution in an off-site reservoir. Pollution loads from point sources (PSs) and nonpoint sources (NPSs) were carefully estimated. Domestic sewage and tourist wastewater were considered the major PS loads in this study. The NPS load evaluation was dependent on the results of a verified watershed model, the stormwater management model (SWMM). The observed data in 2015 and 2016 and supplementary total phosphorous (TP) samplings in upstream rivers in 2018 were used to validate the model results. Model calibration and verification were implemented during dry weather and wet weather to ensure the accuracy of the PS and NPS simulations. The results of this study showed that the average total phosphorous (TP) load generated from within the watershed was 9013 kg/y, and that the TP load from outside the watershed was 4545 kg/y. The percentages of TP loads from NPSs and PSs in the watershed were 83% and 17%, respectively. Finally, we used a verified Vollenweider model to convert the TP loads to the TP concentration in the reservoir. The pollution reduction measures and the associated predicted water quality values were assessed using the verified models.
Optimization of Microwave-Assisted Extraction of Total Phenolic and Total Flavonoid Contents from Fruits of Docynia indica (Wall.) Decne. Using Response Surface Methodology
Authors: Le Xuan , Nguyen Manh , Vu Dinh , Pham Minh , Pham Quoc , Nguyen Quang , Nguyen Tuan , Pham Van , Bach Long , Nguyen Tuong , Tran Quoc
Published: August 2019

Docynia indica (D. indica) shows various useful biological activities, such as antioxidant, anti-inflammatory, antibacterial effects, and positive benefits for human health. Such biological activities relate to the main phytochemicals of D. indica including phenolic and flavonoid. However, isolation for phenolic and flavonoid by popular methods such as hot extraction, soxhlet extraction, and ultrasonic extraction have been relatively ineffective. Therefore, in this study, microwave-assisted extraction (MAE) was used for the extraction of total phenolic and total flavonoid from D. indica. The optimization experiments were conducted based on response surface methodology (RMS) according to a central composite design with four independent variables: extraction time (min), ethanol concentration (%, v/v), microwave power (W), and pH of the solvent. Three dependent variables were total phenolic content (TPC), total flavonoid content (TFC), and yield. The optimal conditions for the extraction of phenolic and flavonoid from D. indica were: extraction time of 50 min, ethanol concentration of 65%, microwave power of 440 W, and solvent pH of 5.4. These conditions corresponded to TPC, TFC, and yield values of 33.57 ± 0.12 (mg GAE/g), 25.01 ± 0.11 (mg QE/g) and 33.44 ± 0.14 (%), respectively.
Optimal Nonlinear Adaptive Control for Voltage Source Converters via Memetic Salp Swarm Algorithm: Design and Hardware Implementation
Authors: Jiang Yueping , Jin Xue , Wang Hui , Fu Yihao , Ge Weiliang , Yang Bo , Yu Tao
Published: August 2019

Voltage source converter (VSC) has been extensively applied in renewable energy systems which can rapidly regulate the active and reactive power. This paper aims at developing a novel optimal nonlinear adaptive control (ONAC) scheme to control VSC in both rectifier mode and inverter mode. Firstly, the nonlinearities, parameter uncertainties, time-varying external disturbances, and unmodelled dynamics can be aggregated into a perturbation, which is then estimated by an extended state observer (ESO) called high-gain perturbation observer (HGPO) online. Moreover, the estimated perturbation will be fully compensated through state feedback. Besides, the observer gains and controller gains are optimally tuned by a recent emerging biology-based memetic salp swarm algorithm (MSSA), the utilization of such method can ensure a desirably satisfactory control performance. The advantage of ONAC is that even though the operation conditions are constantly changing, the control performance can still be maintained to be globally consistent. In addition, it is noteworthy that in rectifier mode only the reactive power and DC voltage are required to be measured, while in inverter mode merely the reactive power and active power have to be measured. At last, in order to verify the feasibility of ONAC in practical application, a hardware experiment is implemented.
Flow Behavior and Hot Processing Map of GH4698 for Isothermal Compression Process
Authors: Chen Rongchuang , Xiao Haifeng , Wang Min , Li Jianjun
Published: August 2019

An in-depth understanding of the flow behaviors of materials deformed at high temperatures is of paramount significance. However, insufficient research on the nickel-based GH4698 alloy has resulted in inaccurate material flow prediction or even cracking in the practical billet opening of GH4698 large forgings. In this study, hot compressions were performed at 950–1150 °C and 0.001–3 s−1. Single-peaked strain-stress curves were obtained under various conditions, owing to dislocation motions in dynamic recrystallizations. The Arrhenius model was formulated to accurately describe the flow stress evolutions and the mean prediction error of the flow stress was 5.90%. Processing maps were constructed at various hot working conditions. It was found that the hot working ability of GH4698 markedly decreased under lower temperatures (950–1080 °C) and higher strain rates (0.1–3 s−1). Optimal thermal processing parameters were suggested. In sum, this study systematically investigated the flow behaviors and hot working ability of GH4698 in isothermal compressions.
The Transcript OPEN Library Political Science Model: A Sustainable Way into Open Access for E-Books in the Humanities and Social Sciences
Authors: Jobmann Alexandra , Schönfelder Nina
Published: August 2019

The strategic goal of the project “National Contact Point Open Access OA2020-DE” is to create the conditions for a large-scale open-access transformation in accordance with the Alliance of German Science Organizations. In close collaboration with the publisher transcript, we developed a business model that strengthens the transformation process for e-books in the humanities and social sciences. It largely addresses the drawbacks of existing models. Moreover, it is manageable, sustainable, transparent, and scalable for both publishers and libraries. This case report describes the setup of the model, its successful implementation for the branch “political science” of transcript in 2019, and provides a Strengths–Weaknesses–Opportunities–Threats (SWOT) analysis. We believe that it has the potential to become one of the major open-access business models for research monographs and anthologies in the humanities and social sciences, especially for non-English e-books.
Parametric Conditions of High Financial Risk in the SME Sector
Authors: Ślusarczyk Beata , Grondys Katarzyna
Published: August 2019

The sector of SME is the major force for the national economic and social development. Financial risk is one of the key threats to the activity of small and medium enterprises. The most common manifestation of the financial risk of SMEs is difficulty in financing the business and lack of funds for development. Banks are unwilling to grant loans to such companies. Moreover, it is the rising operating costs that cause shrinking profits, which may result in corporate debt, difficulty in debt repayment, and consequently, high financial risk of these entities. Numerous differences in conducting the activity of small and large enterprises intensify this risk and mean that the model of credit financing for companies is not adjusted to the capabilities and principles of the operation of small enterprises. Therefore, risk management is one of the most important internal processes in small and medium enterprises. The identification of factors that affect the level of financial risk in these entities is therefore crucial. The main objective of this research was to analyze the impact of selected parametric characteristics of the SME sector on the intensity of financial risk they take. This objective was accomplished on the basis of the survey with the participation of Polish SMEs. In order to test the adopted research assumptions, the linear regression model was used with four continuous variables for each type of the identified financial risk. Based on the final research results, the logit model was obtained for the risk of insufficient profits. It was indicated that both the internationalization of the company and the ability to manage risk are the only factors that affect a high level of risk of low income. The article ends with the discussion and the comparison with some previous research in this area.
Using Forest Inventory Data with Landsat 8 Imagery to Map Longleaf Pine Forest Characteristics in Georgia, USA
Authors: Hogland John , Anderson Nathaniel , Affleck David , St. Peter Joseph
Published: August 2019

This study improved on previous efforts to map longleaf pine (Pinus palustris) over large areas in the southeastern United States of America by developing new methods that integrate forest inventory data, aerial photography and Landsat 8 imagery to model forest characteristics. Spatial, statistical and machine learning algorithms were used to relate United States Forest Service Forest Inventory and Analysis (FIA) field plot data to relatively normalized Landsat 8 imagery based texture. Modeling algorithms employed include softmax neural networks and multiple hurdle models that combine softmax neural network predictions with linear regression models to estimate key forest characteristics across 2.3 million ha in Georgia, USA. Forest metrics include forest type, basal area and stand density. Results show strong relationships between Landsat 8 imagery based texture and field data (map accuracy > 0.80; square root basal area per ha residual standard errors < 1; natural log transformed trees per ha < 1.081). Model estimates depicting spatially explicit, fine resolution raster surfaces of forest characteristics for multiple coniferous and deciduous species across the study area were created and made available to the public in an online raster database. These products can be integrated with existing tabular, vector and raster databases already being used to guide longleaf pine conservation and restoration in the region.
An Improved Stanford Method for Persistent Scatterers Applied to 3D Building Reconstruction and Monitoring
Authors: Yang Bo , Xu Huaping , Liu Wei , Ge Junxiang , Li Chunsheng , Li Jingwen
Published: August 2019

Persistent scatterers interferometric Synthetic Aperture Radar (PS-InSAR) is capable of precise topography measurement up to sub-meter scale and monitoring subtle deformation up to mm/year scale for all the radar image pixels with stable radiometric characteristics. As a representative PS-InSAR method, the Stanford Method for Persistent Scatterers (StaMPS) is widely used due to its high density of PS points for both rural and urban areas. However, when it comes to layover regions, which usually happen in urban areas, the StaMPS is limited locally. Moreover, the measurement points are greatly reduced due to the removal of adjacent PS pixels. In this paper, an improved StaMPS method, called IStaMPS, is proposed. The PS pixels are selected with high density by the improved PS selection strategy. Moreover, the topography information not provided in StaMPS can be accurately measured in IStaMPS. Based on the data acquired by TerraSAR-X/TanDEM-X over the Terminal 3 E (T3 E) site of Beijing Capital International Airport and the Chaobai River of Beijing Shunyi District, a comparison between StaMPS-retrieved results and IStaMPS-retrieved ones was performed, which demonstrated that the density of PS points detected by IStaMPS is increased by about 1.8 and 1.6 times for these two areas respectively. Through comparisons of local statistical results of topography estimation and mean deformation rate, the improvement granted by the proposed IStaMPS was demonstrated for both urban areas with complex buildings or man-made targets and non-urban areas with natural targets. In terms of the spatiotemporal deformation variation, the northwest region of T3 E experienced an exceptional uplift during the period from June 2012 to August 2015, and the maximum uplift rate is approximately 4.2 mm per year.
New Strategies for Time Delay Estimation during System Calibration for UAV-Based GNSS/INS-Assisted Imaging Systems
Authors: LaForest Lisa , Hasheminasab Seyyed Meghdad , Zhou Tian , Flatt John Evan , Habib Ayman
Published: August 2019

The need for accurate 3D spatial information is growing rapidly in many of today’s key industries, such as precision agriculture, emergency management, infrastructure monitoring, and defense. Unmanned aerial vehicles (UAVs) equipped with global navigation satellite systems/inertial navigation systems (GNSS/INS) and consumer-grade digital imaging sensors are capable of providing accurate 3D spatial information at a relatively low cost. However, with the use of consumer-grade sensors, system calibration is critical for accurate 3D reconstruction. In this study, ‘consumer-grade’ refers to cameras that require system calibration by the user instead of by the manufacturer or other high-end laboratory settings, as well as relatively low-cost GNSS/INS units. In addition to classical spatial system calibration, many consumer-grade sensors also need temporal calibration for accurate 3D reconstruction. This study examines the accuracy impact of time delay in the synchronization between the GNSS/INS unit and cameras on-board UAV-based mapping systems. After reviewing existing strategies, this study presents two approaches (direct and indirect) to correct for time delay between GNSS/INS recorded event markers and actual time of image exposure. Our results show that both approaches are capable of handling and correcting this time delay, with the direct approach being more rigorous. When a time delay exists and the direct or indirect approach is applied, horizontal accuracy of 1–3 times the ground sampling distance (GSD) can be achieved without either the use of any ground control points (GCPs) or adjusting the original GNSS/INS trajectory information.