bluuebunny/crossref_metadata_2025 huggingface dataset:
An artificial intelligence-based assessment of soil erosion probability indices and contributing factors in the Abha-Khamis watershed, Saudi Arabia
Authors: Alqadhi Saeed , Mallick Javed , Talukdar Swapan , Alkahtani Meshel
Published: September 2023

Soil erosion is a major problem in arid regions, including the Abha-Khamis watershed in Saudi Arabia. This research aimed to identify the soil erosional probability using various soil erodibility indices, including clay ratio (CR), modified clay ratio (MCR), Critical Level of Soil Organic Matter (CLOM), and principle component analysis based soil erodibility index (SEI). To achieve these objectives, the study used t-tests and an artificial neural network (ANN) model to identify the best SEI model for soil erosion management. The performance of the models were then evaluated using R2, Root Mean Squared Error (RMSE), Mean Squared Error (MSE), and Mean Absolute Error (MAE), with CLOM identified as the best model for predicting soil erodibility. Additionally, the study used Shapley additive explanations (SHAP) values to identify influential parameters for soil erosion, including sand, clay, silt, soil organic carbon (SOC), moisture, and void ratio. This information can help to develop management strategies oriented to these parameters, which will help prevent soil erosion. The research showed notable distinctions between CR and CLOM, where the 25–27% contribution explained over 89% of the overall diversity. The MCR indicated that 70% of the study area had low erodibility, while 20% had moderate and 10% had high erodibility. CLOM showed a range from low to high erodibility, with 40% of soil showing low CLOM, 40% moderate, and 20% high. Based on the T-test results, CR is significantly different from CLOM, MCR, and principal component analysis (PCA), while CLOM is significantly different from MCR and PCA, and MCR is significantly different from PCA. The ANN implementation demonstrated that the CLOM model had the highest accuracy (R2 of 0.95 for training and 0.92 for testing) for predicting soil erodibility, with SOC, sand, moisture, and void ratio being the most important variables. The SHAP analysis confirmed the importance of these variables for each of the four ANN models. This research provides valuable information for soil erosion management in arid regions. The identification of soil erosional probability and influential parameters will help to develop effective management strategies to prevent soil erosion and promote agricultural production. This research can be used by policymakers and stakeholders to make informed decisions to manage and prevent soil erosion.
Rethinking disaster risk for ecological risk assessment
Authors: Singh Gerald G. , Sajid Zaman , Khan Faisal , Mather Charles , Bernhardt Joey R. , Frölicher Thomas L.
Published: September 2023

While disaster events are consequential, they are rare. Ecological risk assessment processes tend to estimate risk through an “expected value” lens that focuses on the most probable events, which can drastically underappreciate the importance of rare events. Here, we show that expected value and average risk-based calculations underappreciate disaster events through questionable assumptions about equally weighing high probability low impact events with low probability high impact events, and in modeling probability as a chance among an ensemble of possible futures when many contexts of ecological risk assessment are focused on a single entity over time. We propose an update to ecological risk assessment that is specifically inclusive of disaster risk potential by adopting analytical processes that estimate the maximum hazard or impact that might be experienced in the future, borrowing from the practice of modeling “Value at Risk” in financial risk contexts. We show how this approach can be adopted in a variety of data contexts, including situations where no quantitative data is available and risk assessment is based on expert judgement, which is common for ecological risk assessment. Increased exposure to environmental variation requires assessment tools to better prepare for, mitigate, and respond to disasters.
A new perspective on Alzheimer’s disease: microRNAs and circular RNAs
Authors: Abidin Shahidee Zainal , Mat Pauzi Nurul Asykin , Mansor Nur Izzati , Mohd Isa Nurul Iffah , Hamid Adila A.
Published: September 2023

microRNAs (miRNAs) play a multifaceted role in the pathogenesis of Alzheimer’s disease (AD). miRNAs regulate several aspects of the disease, such as Aβ metabolism, tau phosphorylation, neuroinflammation, and synaptic function. The dynamic interaction between miRNAs and their target genes depends upon various factors, including the subcellular localization of miRNAs, the relative abundance of miRNAs and target mRNAs, and the affinity of miRNA-mRNA interactions. The miRNAs are released into extracellular fluids and subsequently conveyed to specific target cells through various modes of transportation, such as exosomes. In comparison, circular RNAs (circRNAs) are non-coding RNA (ncRNA) characterized by their covalently closed continuous loops. In contrast to linear RNA, RNA molecules are circularized by forming covalent bonds between the 3′and 5′ends. CircRNA regulates gene expression through interaction with miRNAs at either the transcriptional or post-transcriptional level, even though their precise functions and mechanisms of gene regulation remain to be elucidated. The current stage of research on miRNA expression profiles for diagnostic purposes in complex disorders such as Alzheimer’s disease is still in its early phase, primarily due to the intricate nature of the underlying pathological causes, which encompass a diverse range of pathways and targets. Hence, this review comprehensively addressed the alteration of miRNA expression across diverse sources such as peripheral blood, exosome, cerebrospinal fluid, and brain in AD patients. This review also addresses the nascent involvement of circRNAs in the pathogenesis of AD and their prospective utility as biomarkers and therapeutic targets for these conditions in future research.
Unleashing the power of complement activation: unraveling renal damage in human anti-glomerular basement membrane disease
Authors: Tang Anqi , Zhao Xin , Tao Tian , Xie Dengpiao , Xu Bojun , Huang Youqun , Li Mingquan
Published: September 2023

Anti-glomerular basement membrane (GBM) disease is a rare but life-threatening autoimmune disorder characterized by rapidly progressive glomerulonephritis with or without pulmonary hemorrhage. Renal biopsies of anti-GBM patients predominantly show linear deposition of IgG and complement component 3 (C3), indicating a close association between antigen-antibody reactions and subsequent complement activation in the pathogenesis of the disease. All three major pathways of complement activation, including the classical, lectin, and alternative pathways, are involved in human anti-GBM disease. Several complement factors, such as C3, C5b-9, and factor B, show a positive correlation with the severity of the renal injury and act as risk factors for renal outcomes. Furthermore, compared to patients with single positivity for anti-GBM antibodies, individuals who are double-seropositive for anti-neutrophil cytoplasmic antibody (ANCA) and anti-GBM antibodies exhibit a unique clinical phenotype that lies between ANCA-associated vasculitis (AAV) and anti-GBM disease. Complement activation may serve as a potential “bridge” for triggering both AAV and anti-GBM conditions. The aim of this article is to provide a comprehensive review of the latest clinical evidence regarding the role of complement activation in anti-GBM disease. Furthermore, potential therapeutic strategies targeting complement components and associated precautions are discussed, to establish a theoretical basis for complement-targeted therapies.
Two clip-domain serine protease homologs, cSPH35 and cSPH242, act as a cofactor for prophenoloxidase-1 activation in Drosophila melanogaster
Authors: Jin Qiao , Wang Yang , Yin Haodong , Jiang Haobo
Published: September 2023

Insect phenoloxidases (POs) catalyze phenol oxygenation ando-diphenol oxidation to form reactive intermediates that kill invading pathogens and form melanin polymers. To reduce their toxicity to host cells, POs are produced as prophenoloxidases (PPOs) and activated by a serine protease cascade as required. In most insects studied so far, PPO activating proteases (PAPs) generate active POs in the presence of a highMrcofactor, comprising two serine protease homologs (SPHs) each with a Gly residue replacing the catalytic Ser of an S1A serine protease (SP). These SPHs have a regulatory clip domain at the N-terminus, like most of the SP cascade members including PAPs. InDrosophila, PPO activation and PO-catalyzed melanization have been examined in genetic analyses but it is unclear if a cofactor is required for PPO activation. In this study, we produced the recombinant cSPH35 and cSPH242 precursors, activated them withManduca sextaPAP3, and confirmed their predicted role as a cofactor forDrosophilaPPO1 activation by MP2 (i.e., Sp7). The cleavage sites and mechanisms for complex formation and cofactor function are highly similar to those reported inM. sexta. In the presence of highMrcomplexes of the cSPHs, PO at a high specific activity of 260 U/μg was generatedin vitro. To complement thein vitroanalysis, we measured hemolymph PO activity levels in wild-type flies, cSPH35, and cSPH242 RNAi lines. Compared with the wild-type flies, only 4.4% and 18% of the control PO level (26 U/μl) was detected in the cSPH35 and cSPH242 knockdowns, respectively. Consistently, percentages of adults with a melanin spot at the site of septic pricking were 82% in wild-type, 30% in cSPH35 RNAi, and 53% in cSPH242 RNAi lines; the survival rate of the control (45%) was significantly higher than those (30% and 15%) of the two RNAi lines. These data suggest thatDrosophilacSPH35 and cSPH242 are components of a cofactor for MP2-mediated PPO1 activation, which are indispensable for early melanization in adults.
Constraining the origin of sedimentary organic matter in the eastern Guangdong coast of China using δ13C and δ15N
Authors: Wu Junwen , Chen Jisheng , Wang Cui , Yan Xiuli , Yin Xijie , Liu Qian
Published: September 2023

The source apportionment of organic carbon is of great scientific significance for understanding the carbon sink pattern in the coastal ocean. The concentrations of total organic carbon (TOC) and total nitrogen (TN) and their isotopic compositions (δ13CTOC and δ15NTN) were determined in surface sediments and sediment cores (Y3 and Y5) collected in the eastern Guangdong coast (EGDC) during spring, summer and winter cruises in 2021, in order to determine the distribution and source of sedimentary organic matter. Spatially, TOC and δ13CTOC distributions in the EGDC have contrasting patterns; TOC concentrations decreased and δ13CTOC values increased with distance offshore. Higher TOC concentrations and enriched δ13CTOC were observed in the marine aquaculture area off EGDC. The measured values of δ13CTOC, δ15NTN and the C/N ratio constrained the two dominant sources of organic matter, marine and terrestrial material, in the EGDC. Using a two end−member mixing model, we estimated the proportion of marine organic matter to be 74 ± 20% in spring, 74 ± 16% in summer and 75 ± 12% in winter, respectively. This suggests in situ production of marine phytoplankton dominated the organic carbon pool (average=~74%) in the EGDC sediment. These consistent marine organic matter contributions are also observed in sediment cores, with proportions averaging 59 ± 5% in core Y3 and 66 ± 4% in core Y5. The observed variations in biogeochemical proxies in sediments are thus mainly caused by differing relative contributions of marine and terrestrial organic matter. Additionally, the high TOC inventory in the marine aquaculture area indicates increased carbon storage and carbon fixation capacity compared to the other areas investigated. Overall, this study establishes a background carbon database in the coastal environment that can be used in future studies of blue carbon to achieve carbon neutrality in China.
Summer bottom oxygen depletion dynamics and the associated physical structure in the Bohai Sea
Authors: Zhang Jing , Yang Wei , Song Guisheng , Zhang Haiyan , Zhao Liang
Published: September 2023

Summertime oxygen depletion has been more and more frequently observed in the bottom water of the Bohai Sea in the last decade. Based on comprehensive hydrography and microstructure measurements in summer in the Bohai Sea, the physical structure and bottom dissolved oxygen (DO) dynamics were investigated. The study area is characterized by strong tidal currents and obvious horizontal temperature and DO gradients in the bottom boundary layer. The strong tidal forcing induces large near-bottom turbulent kinetic energy dissipation rates (ϵ ~ 5 × 10-5 W kg-1) which can be well parameterized by the law of the wall. Tidal horizontal advection effects dominate the short-term variations of bottom hydrography. Although the residual current is in a near-perpendicular direction with the horizontal DO gradient (~94°), the horizontal residual DO transport was calculated to be 67.4 mg m-2 d-1 which is ~ 33% of the magnitude of sediment oxygen demand. During the observation period, we observed an intense high-turbidity event leading to a severe drawdown of near-bottom DO concentration (0.16 mg L-1) in 1.5 hours. The DO consumption rate due to this event was then estimated to be ~ 33.3 g m-2 d-1 (1.39 g m-2 h-1) which is two orders of magnitude larger than the sediment oxygen demand. Rapid DO consumption can be induced by the great increase in bioavailable surface area in bottom water and seabed when benthic organic matter is resuspended. This process should be incorporated into the coupled physical-biogeochemical model to improve accuracy in simulating DO depletion.
A novel web-based calculator to predict 30-day all-cause in-hospital mortality for 7,202 elderly patients with heart failure in ICUs: a multicenter retrospective cohort study in the United States
Authors: Wang Zhongjian , Huang Jian , Zhang Yang , Liu Xiaozhu , Shu Tingting , Duan Minjie , Wang Haolin , Yin Chengliang , Cao Junyi
Published: September 2023

Background and aims

Heart failure (HF) is a significant cause of in-hospital mortality, especially for the elderly admitted to intensive care units (ICUs). This study aimed to develop a web-based calculator to predict 30-day in-hospital mortality for elderly patients with HF in the ICU and found a relationship between risk factors and the predicted probability of death.

Methods and results

Data (N = 4450) from the MIMIC-III/IV database were used for model training and internal testing. Data (N = 2,752) from the eICU-CRD database were used for external validation. The Brier score and area under the curve (AUC) were employed for the assessment of the proposed nomogram. Restrictive cubic splines (RCSs) found the cutoff values of variables. The smooth curve showed the relationship between the variables and the predicted probability of death. A total of 7,202 elderly patients with HF were included in the study, of which 1,212 died. Multivariate logistic regression analysis showed that 30-day mortality of HF patients in ICU was significantly associated with heart rate (HR), 24-h urine output (24h UOP), serum calcium, blood urea nitrogen (BUN), NT-proBNP, SpO2, systolic blood pressure (SBP), and temperature (P < 0.01). The AUC and Brier score of the nomogram were 0.71 (0.67, 0.75) and 0.12 (0.11, 0.15) in the testing set and 0.73 (0.70, 0.75), 0.13 (0.12, 0.15), 0.65 (0.62, 0.68), and 0.13 (0.12, 0.13) in the external validation set, respectively. The RCS plot showed that the cutoff values of variables were HR of 96 bmp, 24h UOP of 1.2 L, serum calcium of 8.7 mg/dL, BUN of 30 mg/dL, NT-pro-BNP of 5121 pg/mL, SpO2 of 93%, SBP of 137 mmHg, and a temperature of 36.4°C.

Conclusion

Decreased temperature, decreased SpO2, decreased 24h UOP, increased NT-proBNP, increased serum BUN, increased or decreased SBP, fast HR, and increased or decreased serum calcium increase the predicted probability of death. The web-based nomogram developed in this study showed good performance in predicting 30-day in-hospital mortality for elderly HF patients in the ICU.
Validation of flavivirus infectious clones carrying fluorescent markers for antiviral drug screening and replication studies
Authors: Cherkashchenko Liubov , Gros Nathalie , Trausch Alice , Neyret Aymeric , Hénaut Mathilde , Dubois Gregor , Villeneuve Matthieu , Chable-Bessia Christine , Lyonnais Sébastien , Merits Andres , Muriaux Delphine
Published: September 2023

Flaviviruses have emerged as major arthropod-transmitted pathogens and represent an increasing public health problem worldwide. High-throughput screening can be facilitated using viruses that easily express detectable marker proteins. Therefore, developing molecular tools, such as reporter-carrying versions of flaviviruses, for studying viral replication and screening antiviral compounds represents a top priority. However, the engineering of flaviviruses carrying either fluorescent or luminescent reporters remains challenging due to the genetic instability caused by marker insertion; therefore, new approaches to overcome these limitations are needed. Here, we describe reverse genetic methods that include the design and validation of infectious clones of Zika, Kunjin, and Dengue viruses harboring different reporter genes for infection, rescue, imaging, and morphology using super-resolution microscopy. It was observed that different flavivirus constructs with identical designs displayed strikingly different genetic stabilities, and corresponding virions resembled wild-type virus particles in shape and size. A successful strategy was assessed to increase the stability of rescued reporter virus and permit antiviral drug screening based on quantitative automated fluorescence microscopy and replication studies.
Fungal extracellular vesicle-mediated regulation: from virulence factor to clinical application
Authors: Liu Jie , Hu Xiaoping
Published: September 2023

Invasive fungal disease (IFD) poses a significant threat to immunocompromised patients and remains a global challenge due to limited treatment options, high mortality and morbidity rates, and the emergence of drug-resistant strains. Despite advancements in antifungal agents and diagnostic techniques, the lack of effective vaccines, standardized diagnostic tools, and efficient antifungal drugs contributes to the ongoing impact of invasive fungal infections (IFI). Recent studies have highlighted the presence of extracellular vesicles (EVs) released by fungi carrying various components such as enzymes, lipids, nucleic acids, and virulence proteins, which play roles in both physiological and pathological processes. These fungal EVs have been shown to interact with the host immune system during the development of fungal infections whereas their functional role and potential application in patients are not yet fully understood. This review summarizes the current understanding of the biologically relevant findings regarding EV in host-pathogen interaction, and aim to describe our knowledge of the roles of EV as diagnostic tools and vaccine vehicles, offering promising prospects for the treatment of IFI patients.
Multi-omics analysis reveals substantial linkages between the oral-gut microbiomes and inflamm-aging molecules in elderly pigs
Authors: Qiao Chuanmin , He Maozhang , Wang Shumei , Jiang Xinjie , Wang Feng , Li Xinjian , Tan Shuyi , Chao Zhe , Xin Wenshui , Gao Shuai , Yuan Jingli , Li Qiang , Xu Zichun , Zheng Xinli , Zhao Jianguo , Liu Guangliang
Published: September 2023

Introduction

The accelerated aging of the global population has emerged as a critical public health concern, with increasing recognition of the influential role played by the microbiome in shaping host well-being. Nonetheless, there remains a dearth of understanding regarding the functional alterations occurring within the microbiota and their intricate interactions with metabolic pathways across various stages of aging.

Methods

This study employed a comprehensive metagenomic analysis encompassing saliva and stool samples obtained from 45 pigs representing three distinct age groups, alongside serum metabolomics and lipidomics profiling.

Results

Our findings unveiled discernible modifications in the gut and oral microbiomes, serum metabolome, and lipidome at each age stage. Specifically, we identified 87 microbial species in stool samples and 68 in saliva samples that demonstrated significant age-related changes. Notably, 13 species in stool, including Clostridiales bacterium, Lactobacillus johnsonii, and Oscillibacter spp., exhibited age-dependent alterations, while 15 salivary species, such as Corynebacterium xerosis, Staphylococcus sciuri, and Prevotella intermedia, displayed an increase with senescence, accompanied by a notable enrichment of pathogenic organisms. Concomitant with these gut-oral microbiota changes were functional modifications observed in pathways such as cell growth and death (necroptosis), bacterial infection disease, and aging (longevity regulating pathway) throughout the aging process. Moreover, our metabolomics and lipidomics analyses unveiled the accumulation of inflammatory metabolites or the depletion of beneficial metabolites and lipids as aging progressed. Furthermore, we unraveled a complex interplay linking the oral-gut microbiota with serum metabolites and lipids.

Discussion

Collectively, our findings illuminate novel insights into the potential contributions of the oral-gut microbiome and systemic circulating metabolites and lipids to host lifespan and healthy aging.
Comparative genomics and transcriptome analysis reveals potential pathogenic mechanisms of Microdochium paspali on seashore paspalum
Authors: Jin Peiyuan , Kong Yixuan , Zhang Ze , Zhang Huangwei , Dong Yinglu , Lamour Kurt , Yang Zhimin , Zhou Yuxin , Hu Jian
Published: September 2023

The sparse leaf patch of seashore paspalum (Paspalum vaginatum Sw.) caused by Microdochium paspali seriously impacts the landscape value of turf and poses a challenge to the maintenance and management of golf courses. Little is known about the genome of M. paspali or the potential genes underlying pathogenicity. In this study, we present a high-quality genome assembly of M. paspali with 14 contigs using the Nanopore and Illumina platform. The M. paspali genome is roughly 37.32 Mb in size and contains 10,365 putative protein-coding genes. These encompass a total of 3,830 pathogen-host interactions (PHI) genes, 481 carbohydrate-active enzymes (CAZymes) coding genes, 105 effectors, and 50 secondary metabolite biosynthetic gene clusters (SMGCs) predicted to be associated with pathogenicity. Comparative genomic analysis suggests M. paspali has 672 species-specific genes (SSGs) compared to two previously sequenced non-pathogenic Microdochium species, including 24 species-specific gene clusters (SSGCs). Comparative transcriptomic analyses reveal that 739 PHIs, 198 CAZymes, 40 effectors, 21 SMGCs, 213 SSGs, and 4 SSGCs were significantly up-regulated during the process of infection. In conclusion, the study enriches the genomic resources of Microdochium species and provides a valuable resource to characterize the pathogenic mechanisms of M. paspali.
Theranostic in GLP-1R molecular imaging: challenges and emerging opportunities
Authors: Xie Yang , Wang Yudi , Pei Wenjie , Chen Yue
Published: September 2023

Theranostic in nuclear medicine combines diagnostic imaging and internal irradiation therapy using different therapeutic nuclear probes for visual diagnosis and precise treatment. GLP-1R is a popular receptor target in endocrine diseases, non-alcoholic steatohepatitis, tumors, and other areas. Likewise, it has also made breakthroughs in the development of molecular imaging. It was recognized that GLP-1R imaging originated from the study of insulinoma and afterwards was expanded in application including islet transplantation, pancreatic β-cell mass measurement, and ATP-dependent potassium channel-related endocrine diseases. Fortunately, GLP-1R molecular imaging has been involved in ischemic cardiomyocytes and neurodegenerative diseases. These signs illustrate the power of GLP-1R molecular imaging in the development of medicine. However, it is still limited to imaging diagnosis research in the current molecular imaging environment. The lack of molecular-targeted therapeutics related report hinders its radiology theranostic. In this article, the current research status, challenges, and emerging opportunities for GLP-1R molecular imaging are discussed in order to open a new path for theranostics and to promote the evolution of molecular medicine.
Electrophysiological properties of layer 2/3 pyramidal neurons in the primary visual cortex of a retinitis pigmentosa mouse model (rd10)
Authors: Halfmann Claas , Rüland Thomas , Müller Frank , Jehasse Kevin , Kampa Björn M.
Published: September 2023

Retinal degeneration is one of the main causes of visual impairment and blindness. One group of retinal degenerative diseases, leading to the loss of photoreceptors, is collectively termed retinitis pigmentosa. In this group of diseases, the remaining retina is largely spared from initial cell death making retinal ganglion cells an interesting target for vision restoration methods. However, it is unknown how downstream brain areas, in particular the visual cortex, are affected by the progression of blindness. Visual deprivation studies have shown dramatic changes in the electrophysiological properties of visual cortex neurons, but changes on a cellular level in retinitis pigmentosa have not been investigated yet. Therefore, we used therd10mouse model to perform patch-clamp recordings of pyramidal neurons in layer 2/3 of the primary visual cortex to screen for potential changes in electrophysiological properties resulting from retinal degeneration. Compared to wild-type C57BL/6 mice, we only found an increase in intrinsic excitability around the time point of maximal retinal degeneration. In addition, we saw an increase in the current amplitude of spontaneous putative inhibitory events after a longer progression of retinal degeneration. However, we did not observe a long-lasting shift in excitability after prolonged retinal degeneration. Together, our results provide evidence of an intact visual cortex with promising potential for future therapeutic strategies to restore vision.
Altered gut microbiota and metabolites in untreated myasthenia gravis patients
Authors: Ding Xiao-Jun , Li Hong-Yan , Wang Huaiping , Zhang Xue-Hua , Song Min , Jiang Xiao-Han , Zhang Xu , Yue Yao-Xian , Li Xiao-Hong
Published: September 2023

Objective

The homeostasis of the immune system is influenced by the gut microbiota. Previous studies have reported dysbiosis in the gut microbiota of myasthenia gravis (MG) patients. To investigate potential alterations in gut microbiota and metabolites in newly diagnosed and untreated MG patients, we conducted a case-control study.

Methods

Fecal samples were collected from 11 newly diagnosed and untreated MG patients as well as 11 age-and sex-matched healthy controls. These samples underwent analysis for gut microbiota using 16S ribosomal RNA (rRNA) gene sequencing, while fecal metabolome was analyzed using liquid chromatography-electrospray tandem mass spectrometry system (LC-ESI-MS/MS).

Results

The microbial community richness (observed species) and diversity (Shannon and Simpson indices) were significantly lower in the MG group compared to the control group. Microbiota composition analysis revealed significant differences between the MG and control groups at phylum, family, and genus levels. Linear discriminant analysis effect size (LEfSe) analysis showed a substantial decrease in abundance of the genus Faecalibacterium within the MG group. Fecal metabolome analysis identified three up-regulated metabolites involved in amino acid metabolism (taurine, creatinine, L-carnitine), one up-regulated metabolite involved in lipid metabolism (oleic acid), with correlation analysis indicating a positive association between Faecalibacterium abundance and creatinine levels.

Conclusion

Our findings suggest that dysbiosis already exists in newly diagnosed and untreated MG patients, implying that dysbiosis within the gut microbiota may be an initiating factor contributing to MG pathogenesis. Furthermore, F. prausnitzii may hold promise as a probiotic for treating MG.
Multimodal evaluation of the bloodstream alteration before and after combined revascularization for Moyamoya disease
Authors: Cao Lei , Yuan Xiaoli , Dong Yang , Wang Zeming , Guo Mengguo , Li Dongpeng , Zhang Manxia , Yan Dongming , Yang Bo , Li Hongwei
Published: September 2023

Objective

This study aimed to explore the hemodynamic changes before and after anastomosis in patients with Moyamoya disease (MMD) using multiple models.

Methods

We prospectively enrolled 42 MMD patients who underwent combined revascularization. Intraoperative FLOW800 was performed before and after anastomosis, and parameters was collected, including maximum intensity, delay time, rise time, slope, blood flow index, and microvascular transit time (MVTT). Additionally, preoperative and postoperative hemodynamic parameters were measured using color Doppler ultrasonography (CDUS), including peak systolic velocity, end-diastolic velocity, resistance index (RI), pulsatility index (PI), and flow volume. Subsequently, the correlation between FLOW800 and CDUS parameters was explored.

Results

A total of 42 participants took part with an average age of 46.5 years, consisting of 19 men and 23 women. The analysis of FLOW800 indicated that both the delay time and rise time experienced a substantial decrease in both the recipient artery and vein. Additionally, the MVTT was found to be significantly reduced after the surgery (5.7 ± 2.2 s vs. 4.9 ± 1.6, p = 0.021). However, no statistically significant differences were observed among the other parameters. Similarly, all postoperative parameters in CDUS hemodynamics exhibited significant alterations in comparison to the preoperative values. The correlation analysis between FLOW800 and CDUS parameters indicated a significant association between MVTT and RI and PI, no significant relationships were found among the other parameters in the two groups.

Conclusion

The hemodynamic outcomes of the donor and recipient arteries demonstrated significant changes following bypass surgery. The parameter of time appears to be more precise and sensitive in assessing hemodynamics using FLOW800. Multiple evaluations of hemodynamics could offer substantial evidence for perioperative management.
Sarcopenia defined with L3-SMI is an independent predictor of survival in male patients with ARLD in mainland China
Authors: Zhang Yu , Wei Liangui , Chang Chunyan , Duan Fangfang , Quan Min , Yang Song
Published: September 2023

Background

The burden of alcohol-related liver disease (ARLD) is increasing in China. Patients with ARLD are more likely to have comorbid sarcopenia, which may impair their survival. This study aimed to evaluate the relationship between the prognoses of patients with ARLD and sarcopenia, identified using the skeletal muscle index at the third lumbar vertebra level (L3-SMI).

Methods

Hospitalized patients with ARLD were retrospectively enrolled between 2015 and 2018 and followed up for 24 months to evaluate their survival profiles. Cox proportional hazards regression models were used to estimate patient survival factors. A receiver operating characteristic curve was created to identify the cut-off point of the L3-SMI for predicting the prognoses of Chinese patients with ARLD.

Results

The study enrolled 168 male patients with ARLD who were followed-up for 24 months or until a study endpoint was met. The overall L3-SMI in patients with ARLD was 42.61 ± 9.15 cm2/m2, and 42.86% (72/168) of patients with ARLD were comorbid with sarcopenia. The overall survival in patients with ARLD was 77.38% at 24 months. The survival rate of patients with sarcopenia was lower than that of patients without sarcopenia (66.67% vs. 85.42%, p = 0.004). Multiple Cox regression analysis showed that sarcopenia, abstinence, and baseline creatinine level were independent prognostic factors of 24-month survival with hazard ratios (95% confidence intervals) of 2.022 (1.025–3.991), 0.275 (0.122–0.617), and 1.018 (1.008–1.027), respectively. The cut-off value of the L3-SMI for predicting 24-month survival was 40.0 cm2/m2 for male patients with ARLD.

Conclusion

Sarcopenia is an independent mortality risk factor in male patients with ARLD in mainland China. Early diagnosis and intervention of sarcopenia are important for optimizing the management of patients with ARLD.
The potential use of Indian rice flour or husk in fortification of pan bread: assessing bread’s quality using sensory, physicochemical, and chemometric methods
Authors: Elkatry Haiam O. , El-Beltagi Hossam S. , Ahmed Abdelrahman R. , Mohamed Heba I. , Al-Otaibi Hala Hazam , Ramadan Khaled M. A. , Mahmoud Mohamed A. A.
Published: September 2023

Hassawi rice is an Indica variety cultivated in Saudi Arabia with a higher nutritional value than the commercial Basmati rice varieties. The present study has investigated the feasibility of combining Hassawi rice flour (HRF) or husk (HRHF), an abundant byproduct, with wheat flour to produce nutritious economical pan bread. To achieve this aim, the physicochemical properties of HRF and HRHF were assessed using techniques such as UPLC-tandem MS, ICP-OES, and colorimeter. The proximate composition (moisture, crude fiber, and ash) and mineral contents of HRHF are significantly (p < 0.05) higher than HRF. On the other hand, the compounds p-coumaric acid, vanillic acid, γ- and δ-tocotrienols, and γ-oryzanol were unique to HRF. We further determined the changes in sensory, technological, and physicochemical properties of wheat flour bread substituted with 5%, 10%, and 15% of HRF or HRHF. The rheological tests showed that the addition of HRF and HRHF increased dough development and stability time. Further, substituting wheat flour for HRF and HRHF at levels higher than 10% affected sensory attributes, such as color, taste, odor, flavor, and appearance. These changes, however, were not always at a significant level. The causes of the differences in properties between control and fortified bread samples were investigated by chemometric methods. Samples of bread +HRF at 5 and 10% had comparable overall profiles to the control. On the other hand, bread +HRHF samples proved to retain higher concentrations of bioactive molecules compared to the control bread. Our findings shed light on the possible use of rice husk fibers in baking goods, notably pan bread. Furthermore, by integrating rice husk fibers into baked goods, we may boost their health benefits while also contributing to the long-term use of agricultural waste.
Ameliorative effect of black tea extract on the skin of D-galactose-induced aging mice
Authors: Zheng Xiaojie , Deng Wenbin , Wang Xinzhou , Wu Zufang , Li Chong , Zhang Xin
Published: September 2023

Aging is a universal and irreversible process, and the skin is an important feature that reflects the aging of the organism. Skin aging has been a focus of attention in recent years because it leads to changes in an individual’s external features and the loss of many important biological functions. This experiment investigated the improvement effect of black tea extract (BTE) on the skin of aging mice under D-galactose induction. After 6 weeks of administration, the changes in skin bio-chemical indices and tissue structure were compared with the blank and positive control groups. It was observed that BTE increased water and hyaluronic acid (HA) content, decreased malondialdehyde (MDA) content, enhanced superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) activities in the skin of aging mice, and improved the structure of aging damaged skin tissues and increased the content of total collagen. The experimental results showed that BTE can play a significant anti-aging effect on the skin, which can be used as a functional food for aging inhibition.
PET imaging with [68Ga]-labeled TGFβ-targeting peptide in a mouse PANC-1 tumor model
Authors: Li Yong , Zhao Hong , Hu Shan , Zhang Xichen , Chen Haojian , Zheng Qihuang
Published: September 2023

Purpose

Transforming growth factor β (TGFβ) is upregulated in many types of tumors and plays important roles in tumor microenvironment construction, immune escape, invasion, and metastasis. The therapeutic effect of antibodies and nuclide-conjugated drugs targeting TGFβ has not been ideal. Targeting TGFβ with small-molecule or peptide carriers labeled with diagnostic/therapeutic nuclides is a new development direction. This study aimed to explore and confirm the imaging diagnostic efficiency of TGFβ-targeting peptide P144 coupled with [68Ga] in a PANC-1 tumor model.

Procedures

TGFβ-targeting inhibitory peptide P144 with stable activity was prepared through peptide synthesis and screening, and P144 was coupled with biological chelator DOTA and labeled with radionuclide [68Ga] to achieve a stable TGFβ-targeting tracer [68Ga]Ga-P144. This tracer was first used for positron emission tomography (PET) molecular imaging study of pancreatic cancer in a mouse PANC-1 tumor model.

Results

[68Ga]Ga-P144 had a high targeted uptake and relatively long uptake retention time in tumors and lower uptakes in non-target organs and backgrounds. Target pre-blocking experiment with the cold drug P144-DOTA demonstrated that the radioactive uptake with [68Ga]Ga-P144 PET in vivo, especially in tumor tissue, had a high TGFβ-targeting specificity. [68Ga]Ga-P144 PET had ideal imaging efficiency in PANC-1 tumor-bearing mice, with high specificity in vivo and good tumor-targeting effect.

Conclusion

[68Ga]Ga-P144 has relatively high specificity and tumor-targeted uptake and may be developed as a promising diagnostic tool for TGFβ-positive malignancies.
Prognostic and therapeutic impact of the KRAS G12C mutation in colorectal cancer
Authors: Qunaj Lindor , May Michael S. , Neugut Alfred I. , Herzberg Benjamin O.
Published: September 2023

KRAS G12C mutations are critical in the pathogenesis of multiple cancer types, including non-small cell lung (NSCLC), pancreatic ductal adenocarcinoma (PDAC), and colorectal (CRC) cancers. As such, they have increasingly become a target of novel therapies in the management of these malignancies. However, the therapeutic success of KRAS G12C inhibitors to date has been far more limited in CRC and PDAC than NSCLC. In this review, we briefly summarize the biochemistry of KRAS targeting and treatment resistance, highlight differences in the epidemiology of various G12C-mutated cancers, and provide an overview of the published data on KRAS G12C inhibitors for various indications. We conclude with a summary of ongoing clinical trials in G12C-mutant CRC and a discussion of future directions in the management of this disease. KRAS G12C mutation, targeted therapies, colorectal cancer, non-small cell lung cancer, pancreatic cancer, drug development.
The application of artificial technology in pediatric pyeloplasty the efficacy analysis of robotic-assisted laparoscopic pyeloplasty in the treatment of ureteropelvic junction obstruction
Authors: Hu Zhongli , Chen Shan , Wang Zhihong , Xu Di , Zhang Xiaolang , Lin Yang , Zhang Lin , Wang Jianbin , Li Lizhi
Published: September 2023

Objective

To investigate the clinical effect of the da Vinci robotic-assisted laparoscopic pyeloureteroplasty (RALP) in treating pediatric ureteropelvic junction obstruction (UPJO).

Methods

We retrospectively analyzed clinical data from 32 children with UPJO who suffered from RALP in our hospital from October 2020 to February 2023, compared with those treated with common laparoscopy at the same time. After the establishment of the robotic arm, a mesenteric approach was performed after entering the abdominal cavity to focus on the lesion site. The dilated renal pelvis was then cut and the stenotic ureter was removed; the anastomosis and the incision were sutured by layer.

Results

A total of 62 children (44 boys and 20 girls) with a median age of 14 months (ranging from 3 to 38 months) were included. All 62 cases had hydronephrosis caused by unilateral UPJO, and the surgery was successfully completed without conversion to open. All intraoperative blood losses amounted to less than 10 ml. In the RALP group, the average operative duration was 131.28 min (ranging from 108 to 180 min). The average catheter time was 3.66 days (ranging from 2 to 7 days). The average hematuria time was 3.84 days (ranging from 2 to 6 days). The average postoperative hospital stay was 7.8 days (ranging from 6 to 12 days). The average hospitalization costs were 59,048.31 yuan (ranging from 50,484 to 69,977 yuan). The double-J tube was removed 1 month after surgery. Only one patient suffered from complications, developing a urinary tract infection 4 weeks after surgery, and was cured with the administration of oral cefaclor anti-inflammatory drugs for 3 days. All patients were followed up for 2–28 months, with a median follow-up time of 12 months. The thickness of the renal cortex was increased after surgery [(1.95 ± 0.24) vs. (4.82 ± 0.50)] cm, and the isotope renograms revealed a definite recovery of the split renal function [(28.32 ± 1.95) vs. (37.01 ± 2.71)]%.

Conclusion

The robotic-assisted laparoscopic pyeloureteroplasty (RALP) in the treatment of children with upper ureteral obstruction has overall clinical efficiency. With technological advancements and an increased number of experienced surgeons, robotic surgery may become a new trend in surgery.
Association between interruption of intervention and language performance in young children with language delay—a cohort study during COVID-19 pandemic
Authors: Hsu Shao-Chih , Wong Alice May-Kuen
Published: September 2023

Introduction

To assess the association between a three-month interruption of language intervention programs and the language performance of children with language delay during the COVID-19 pandemic, and to identify which children are more vulnerable to such interruptions.

Materials and methods

This is a retrospective study involving 33 children with language delay who experienced a three-month suspension of language interventions due to the COVID-19 pandemic. We collected their demographic data and language performance scores from the Comprehensive Developmental Inventory for Infants and Toddlers—Diagnostic test (CDIIT-DT) at four different time points. The scores were analyzed using a Wilcoxon Signed Ranks test.

Results

The median scores of language comprehension and overall language ability showed a decreasing trend during the interruption period. However, resuming interventions post-interruption showed a statistically significant increase in all language domains. Children in the borderline delay group (CDIIT-DT DQ scores between 71 and 85) were more likely to experience a decline in their language abilities during the interruption.

Discussion

This is the first study to reveal a decreasing trend in language performance during interruption periods, and highlighting the significance of post-interruption language interventions in facilitating improvements. Furthermore, our study brings attention to the heightened vulnerability of children exhibiting borderline language delay in overall language ability tests when faced with interruptions in language interventions.
Impact of CytoSorb and CKRT on hemodynamics in pediatric patients with septic shock: the PedCyto study
Authors: Bottari Gabriella , Guzzo Isabella , Cappoli Andrea , Labbadia Raffaella , Perdichizzi Salvatore , Serpe Carmela , Creteur Jacques , Cecchetti Corrado , Taccone Fabio Silvio
Published: September 2023

Background

There is a lack of data to support the use of hemoadsorption in pediatric septic shock. The aim of our study was to assess the effectiveness and safety of CytoSorb therapy in this setting.

Methods

Phase II interventional single arm pilot study in which 17 consecutive children admitted with septic shock who required continuous kidney replacement therapy (CKRT) and weighed ≥10 kg were included. A CytoSorb (CytoSorbents Inc, New Jersey, USA) hemoadsorption cartridge was added to the CKRT every 24 h for a maximum of 96 h. A control group of 13 children with septic shock treated with CKRT but not hemoadsorption at Children's Hospital Bambino Gesù and enrolled in the EuroAKId register was selected as an historical cohort. The primary outcome of the study was a reduction in vasopressor or inotrope dose of >50% from baseline by the end of CytoSorb therapy. Secondary outcomes included hemodynamic and biological changes, changes in severity scores, and 28-day mortality.

Results

There were significant decreases in the Vasoactive Inotropic Score (VIS) and the Pediatric Logistic Organ Dysfunction 2 (PELOD-2) score at 72 and 96 h from the start of the CytoSorb therapy compared to baseline; the reductions were larger in the hemoadsorption group than in the control group (historical cohort). 28-day mortality was lower, although not significantly, in the hemoadsorption group when compared to the control group (5/17 [29%] vs. 8/13 [61%] OR 0.26 [95% CI: 0.05–1.2]; p = 0.08).

Conclusions

CytoSorb therapy may have some benefits in pediatric patients with septic shock. Future larger randomized trials are needed in this setting.

Clinical Trial Registration

https://clinicaltrials.gov/ct2/show/NCT05658588, identifier (Clinicaltrials.gov NCT05658588).
Modeling solar chromospheric spicules with intense lasers
Authors: Wang Jianzhao , Zhong Jiayong , An Weiming , Zhou Weimin , Wang Chen , Zhang Bo , Ping Yongli , Sun Wei , Yuan Xiaoxia , Tang Pengfei , Zhang Yapeng , Zhang Qian , Xing Chunqing , Liu Zhengdong , Yu Jiacheng , Xiong Jun , He Shukai , Hutton Roger , Gu Yuqiu , Zhao Gang , Zhang Jie
Published: September 2023

Solar spicules are small-scale jet-like structures in the lower solar atmosphere. Currently, the formation of these widely distributed structures lacks a complete explanation. It is still unclear whether they play an essential role in corona heating. Here, based on the magnetohydrodynamic scaling transformation relation, we perform experiments with the interaction of a high power laser with a one-dimensional sinusoidal modulated target to model solar spicules. We observe several spicule-like structures with alternating polarity magnetic fields around them. Magnetohydrodynamic simulations with similar parameters show the detail information during the spicules’ formation. The results suggest that the so-called strong pulse model can lead to the formation of the solar spicules. The magnetic reconnection process may also play a part and lead to additional heating and brightening phenomena.
GWAS with principal component analysis identify QTLs associated with main peanut flavor-related traits
Authors: Zhang Hui , Dean Lisa , Wang Ming Li , Dang Phat , Lamb Marshall , Chen Charles
Published: September 2023

Peanut flavor is a complex and important trait affected by raw material and processing technology owing to its significant impact on consumer preference. In this research, principal component analysis (PCA) on 33 representative traits associated with flavor revealed that total sugars, sucrose, and total tocopherols provided more information related to peanut flavor. Genome-wide association studies (GWAS) using 102 U.S. peanut mini-core accessions were performed to study associations between 12,526 single nucleotide polymorphic (SNP) markers and the three traits. A total of 7 and 22 significant quantitative trait loci (QTLs) were identified to be significantly associated with total sugars and sucrose, respectively. Among these QTLs, four and eight candidate genes for the two traits were mined. In addition, two and five stable QTLs were identified for total sugars and sucrose in both years separately. No significant QTLs were detected for total tocopherols. The results from this research provide useful knowledge about the genetic control of peanut flavor, which will aid in clarifying the molecular mechanisms of flavor research in peanuts.