bluuebunny/crossref_metadata_2025 huggingface dataset:
Analysis of Cuticular Lipids of the Pharaoh Ant (Monomorium pharaonis) and Their Selective Adsorption on Insecticidal Zeolite Powders
Authors: Van Den Noortgate Heleen , Lagrain Bert , Wenseleers Tom , Martens Johan A.
Published: September 2018

The pharaoh ant is a notorious and hard to eradicate pest, which poses a threat in hospitals, spreading pathogens and contaminating sterile equipment. When applied on ants, zeolites adsorb part of their epicuticular wax layer. The ants are then vulnerable to desiccation, since this layer regulates water exchange. We analyzed the chemical composition of this wax layer using GC-MS (Gas Chromatography-Mass Spectrometry). A hexane wash of M. pharaonis foragers resulted in the identification of 53 components, four of which were not previously defined in Monomorium species. Selective adsorption of specific compounds on zeolites assisted in the identification of compounds which could not be separated on the GC column and allowed for the identification of three additional compounds. Zeolites show different affinities for the wax compounds depending on pore structure and chemical composition. Selective adsorption of alkanes on zeolites is also investigated in the fields of refinery processes and catalysis. Pore mouth and key lock adsorption mechanisms and selectivity according to molecular weight and branching, investigated in these fields, are also involved in adsorption processes of epicuticular waxes. The insecticidal activity of a zeolite is related to adsorption selectivity rather than capacity. One of the best adsorbing zeolites showed limited insecticidal activity and can be considered as a non-lethal alternative for epicuticular wax sampling.
A New Nearest Centroid Neighbor Classifier Based on K Local Means Using Harmonic Mean Distance
Authors: Mehta Sumet , Shen Xiangjun , Gou Jiangping , Niu Dejiao
Published: September 2018

The K-nearest neighbour classifier is very effective and simple non-parametric technique in pattern classification; however, it only considers the distance closeness, but not the geometricalplacement of the k neighbors. Also, its classification performance is highly influenced by the neighborhood size k and existing outliers. In this paper, we propose a new local mean based k-harmonic nearest centroid neighbor (LMKHNCN) classifier in orderto consider both distance-based proximity, as well as spatial distribution of k neighbors. In our method, firstly the k nearest centroid neighbors in each class are found which are used to find k different local mean vectors, and then employed to compute their harmonic mean distance to the query sample. Lastly, the query sample is assigned to the class with minimum harmonic mean distance. The experimental results based on twenty-six real-world datasets shows that the proposed LMKHNCN classifier achieves lower error rates, particularly in small sample-size situations, and that it is less sensitive to parameter k when compared to therelated four KNN-based classifiers.
The Development of Cytogenetic Maps for Malaria Mosquitoes
Authors: Artemov Gleb N. , Stegniy Vladimir N. , Sharakhova Maria V. , Sharakhov Igor V.
Published: September 2018

Anopheline mosquitoes are important vectors of human malaria. Next-generation sequencing opens new opportunities for studies of mosquito genomes to uncover the genetic basis of a Plasmodium transmission. Physical mapping of genome sequences to polytene chromosomes significantly improves reference assemblies. High-resolution cytogenetic maps are essential for anchoring genome sequences to chromosomes as well as for studying breakpoints of chromosome rearrangements and chromatin protein localization. Here we describe a detailed pipeline for the development of high-resolution cytogenetic maps using polytene chromosomes of malaria mosquitoes. We apply this workflow to the refinement of the cytogenetic map developed for Anopheles beklemishevi.
Effects of Statin Use in Advanced Chronic Kidney Disease Patients
Authors: Huang Tao-Min , Wu Vin-Cent , Lin Yu-Feng , Wang Jian-Jhong , Shiao Chih-Chung , Chen Likwang , Chueh Shih-Chieh , Chueh Eric , Yang Shao-Yu , Lai Tai-Shuan , Lin Shuei-Liong , Chu Tzong-Shinn , Wu Kwan-Dun ,   On Behalf of the National Taiwan University Hospital Study Group on Acute Renal Failure (NSARF)
Published: September 2018

Although statin treatment is recommended for patients with chronic kidney disease (CKD) stages I–IV, its potential benefits have not been reported in advanced CKD patients. Non-diabetic patients with advanced CKD (pre-dialysis patients, estimated glomerular filtration rate <15 mL/min/1.73 m2) were enrolled from a National Health Insurance Research Database with a population of 23 million. Statin users and non-users were matched using propensity scoring and analyzed using Cox proportional hazards models, taking mortality as a competing risk with subsequent end-stage renal disease (ESRD) and statin doses as time-dependent variables. A total of 2551 statin users and 7653 matched statin non-users were identified from a total 14,452 patients with advanced CKD. Taking mortality as a competing risk, statin use did not increase the risk of new-onset diabetes mellitus (NODM) or decrease the risk of de novo major adverse cardiovascular events (MACE), but reduced all-cause mortality (hazard ratio (HR) = 0.59 [95% CI 0.42–0.84], p = 0.004) and sepsis-related mortality (HR = 0.53 [95% CI 0.32–0.87], p = 0.012). For advanced CKD patients, statin was neither associated with increased risks of developing NODM, nor with decreased risk of de novo MACE occurrence, but with a reduced risk of all-cause mortality, mainly septic deaths.
Pre-Procedural Statin Use Is Associated with Improved Long-Term Survival and Reduced Major Cardiovascular Events in Patients Undergoing Carotid Artery Stenting: A Retrospective Study
Authors: Colussi GianLuca , Zuttion Francesca , Bais Bruno , Dolso Pierluigi , Valente Mariarosaria , Gigli Gian Luigi , Gasparini Daniele , Sponza Massimo , Catena Cristiana , Sechi Leonardo A. , Cavarape Alessandro
Published: September 2018

Carotid artery stenting (CAS) is a minimal invasive procedure used to resolve carotid occlusion that can be affected by peri-procedural complications. Statin use before CAS has shown to reduce peri-procedural risk and improve survival, though time-dependent cofactors that influence mortality has not been considered. The aim of this study was to evaluate long-term survival of patients who undergo CAS considering new occurred major adverse cardiovascular event (MACE) as time-dependent cofactor. In this study, 171 high cardiovascular risk patients (age 72 ± 8 years, 125 males) were enrolled after CAS procedure and were followed for a median of 8.4 years. Death occurred in 44% of patients with a mean time to death of 69 ± 39 months and MACE in 34% with a mean time of 35 ± 42 months. In patients who used or not statins at baseline, death occurred in 33% and 65%, respectively (p < 0.001). Survival analysis showed that statin use reduced risk of death (hazard ratio HR 0.36, 95% confidence interval CI 0.23–0.58, p < 0.0001). Including MACE as time-dependent variable did not change beneficial effects of statins. Additionally, statin use was associated with a protective effect on MACE (HR 0.48, 95% CI 0.27–0.85, p = 0.012); particularly, the prevalence of stroke was reduced by 59% (p = 0.018). In multivariate analysis, effects of statins were independent of demographic and anthropometric variables, prevalence of cardiovascular risk factors, renal function, antiplatelet use, and MACE occurrence. In conclusion, use of statins before CAS procedure is associated with increased long-term survival and reduced MACE occurrence. This evidence supports the hypothesis that statin use before CAS might be beneficial in high risk patients.
A Modeling Study on the Oil Spill of M/V Marathassa in Vancouver Harbour
Authors: Zhong Xiaomei , Niu Haibo , Wu Yongsheng , Hannah Charles , Li Shihan , King Thomas
Published: September 2018

The M/V Marathassa oil spill occurred on 8 April 2015 in the English Bay. In the present study, the trajectory and the transport mechanism of the spilled oil have been studied by using the three-dimensional and particle-based Oil Spill Contingency and Response (OSCAR) model forced by the Finite-Volume Community Ocean Model (FVCOM). FVCOM provided the hydrodynamic variables used by the oil spill model of OSCAR. The results showed that the fraction of the oil on the water surface and on the shoreline, as well as the amount of oil recovered were affected by the time of the initial release, the overall duration of the discharge, wind and recovery actions. The hindcast study of the M/V Marathassa oil spill showed that the likely starting time for the discharge was between 14:00 and 15:00, on 8 April 2015. The release may have lasted for a relatively long time (assumed to be 22 h in this study). The results of modeling in this study were found reasonably acceptable allowing for further application in risk assessment studies in the English Bay and Vancouver Harbour. The trajectory of the spill was mainly controlled by the tidal currents, which were strongly sensitive to the local coastline and topography of First Narrows and that in the central harbour. The model results also suggested that a high-resolution model, which was able to resolve abrupt changes in the coastlines and topography, was necessary to simulate the oil spill in the harbour.
Modelling Farm Growth and Its Impact on Agricultural Land Use: A Country Scale Application of an Agent-Based Model
Authors: Beckers Veronique , Beckers Jeroen , Vanmaercke Matthias , Van Hecke Etienne , Van Rompaey Anton , Dendoncker Nicolas
Published: September 2018

The ongoing economic pressure on farmers has resulted in lower gross margins, lower income, and a continuous decrease in the number of farmers in large parts of the world. Most remaining farmers upscale their activities by taking over the land of their former competitors, resulting in a decrease in agricultural employment and an increase in average farm size, accompanied by specialisation and new management techniques. Understanding these significant trends and their impact on the land use and environment requires a deeper knowledge of the mechanisms involved and the impacts of different policy measures. These processes are ideally represented through agent-based modelling. Currently, agent-based models are rarely for larger regions. This paper presents ADAM (Agricultural Dynamics through Agent-based Modelling), using it for the case study of Belgium. ADAM was created to obtain insights in past and current agricultural trends and to explore possible effects of policy measures. ADAM simulates the evolution of a farmer population and their farms at a fine scale on the country level. It produces yearly outputs on the number of farms, their size, and the type of farming activity on every parcel. Results show that ADAM is capable of adequately modelling a farmer population according to past trends and that it can be used to explore the results of a business-as-usual scenario, therefore showing the possibility of creating agent-based models for larger scale real-world applications.
The Actors, Rules and Regulations Linked to Export Horticulture Production and Access to Land and Water as Common Pool Resources in Laikipia County, Northwest Mount Kenya
Authors: Ngutu Mariah , Bukachi Salome , Olungah Charles Owuor , Kiteme Boniface , Kaeser Fabian , Haller Tobias
Published: September 2018

Agriculture is the backbone of Kenya’s economy, supporting up to 80% of rural livelihoods. Kenya’s export horticulture is currently the leading agriculture subsector in Kenya and is regarded as an agro-industrial food system based on the economies of scale, producing for mass markets outside of the production area. Much of the food consumed from Kenya’s export horticulture sector has undergone multiple transformations and been subject to a host of formal and informal institutions (rules, regulations, standards, norms and values). Kenya’s export horticulture production, driven by rising global demands, has expanded beyond the ‘traditional’ mountainous high yielding areas into arid and semi-arid (ASALs) zones such as Laikipia County, Northwest of Mount Kenya. An anthropological study of export horticulture viewed as an agro-industrial food system in Laikipia County was carried out utilizing the new institutionalism theory in anthropology to explore the actors, rules and regulations linked to export horticulture production and access to common pool resources. The study employed qualitative data collection methods to collect data over an extended field work period of eight months. The data from 40 in-depth interviews complemented by unstructured observations, four focus group discussions and five key informant interviews was transcribed, coded and analyzed thematically based on the grounded theory approach. This paper, therefore, presents findings from the qualitative case study on the actors as well as the rules and regulations (the institutional settings) of export horticulture production and access to common pool resources from an emic perspective of the involved actors. The formal and informal rules and regulations which form the institutional setting in this food system are viewed as changing and defining the operations of the food system’s access and management of common pool resources, namely water and land. With the agro-industrial food system competing with local food systems such as agro-pastoralism and small holder agriculture for these scarce resources in a semi-arid zone, there is potential for conflict and reduced production, as well as overall benefits to the different actors in the study area.
Laboratory and Field Investigation of the Feasibility of Crumb Rubber Waste Application to Improve the Flexibility of Anti-Rutting Performance of Asphalt Pavement
Authors: Li Hongyin , Jiang Hailong , Zhang Wenwu , Liu Peng , Wang Shanshan , Wang Fei , Zhang Jizhe , Yao Zhanyong
Published: September 2018

Resistance of asphalt mix to low-temperature cracking and rutting at high temperature is very important to ensure the service performance of asphalt pavement under seasonal changes in temperature and loading. However, it is challenging to balance the improvement of such resistance by using additives, e.g., anti-rutting agent (ARA). This study focuses on improving the flexibility of anti-rutting asphalt mix by incorporating crumb rubber (CR) and ARA. The properties of the prepared modified asphalt mix were evaluated in the laboratory by performing wheel tracking, three-point bending, indirect tensile, and uniaxial compression tests. The experimental results showed that the dynamic stability of modified asphalt mix was significantly increased due to the addition of ARA and further improved by incorporating CR. The maximum bending strain at −10 °C was increased due to the contribution of CR. The results of indirect tensile strength and resilient modulus further indicated that the CR-modified anti-rutting mixture was more flexible. Moreover, the field observation and evaluation indicated that the CR-modified anti-rutting asphalt pavement met the standard requirements, better than normal asphalt mixture in many parameters. A conclusion can be made that incorporating CR in asphalt mixture prepared with ARA can improve pavement performance at both high and low in-service temperatures.
Electricity Consumption Estimation of the Polymer Material Injection-Molding Manufacturing Process: Empirical Model and Application
Authors: Elduque Ana , Elduque Daniel , Pina Carmelo , Clavería Isabel , Javierre Carlos
Published: September 2018

Polymer injection-molding is one of the most used manufacturing processes for the production of plastic products. Its electricity consumption highly influences its cost as well as its environmental impact. Reducing these factors is one of the challenges that material science and production engineering face today. However, there is currently a lack of data regarding electricity consumption values for injection-molding, which leads to significant errors due to the inherent high variability of injection-molding and its configurations. In this paper, an empirical model is proposed to better estimate the electricity consumption and the environmental impact of the injection-molding process. This empirical model was created after measuring the electricity consumption of a wide range of parts. It provides a method to estimate both electricity consumption and environmental impact, taking into account characteristics of both the molded parts and the molding machine. A case study of an induction cooktop housing is presented, showing adequate accuracy of the empirical model and the importance of proper machine selection to reduce cost, electricity consumption, and environmental impact.
Taguchi Grey Relational Analysis for Multi-Response Optimization of Wear in Co-Continuous Composite
Authors: Achuthamenon Sylajakumari Prasanth , Ramakrishnasamy Ramesh , Palaniappan Gopalakrishnan
Published: September 2018

Co-continuous composites have potential in friction and braking applications due to their unique tribological characteristics. The present study involves Taguchi grey relational analysis-based optimization of wear parameters such as applied load, sliding speed and sliding distance, and their effect on dry sliding wear performance of AA6063/SiC co-continuous composite manufactured by gravity infiltration. A Taguchi L9 orthogonal array was designed and nine experimental runs were performed based on the designed experiments. The coefficient of wear and specific wear rate were recorded for each experiment. Based on the average responses computed from Taguchi grey relational analysis, an applied load of 60 N, sliding speed of 1 m/s and sliding distance of 1000 m were estimated to be the optimal parameters. An Analysis of Variance (ANOVA) was conducted to identify the predominant factor and established all the three factors as being significant. The sliding distance was found to have the highest significant influence of 61.05% on the wear of the C4 composite. Confirmation experiments conducted using the optimal parameters indicated an improvement of 35.25% in grey relational grade. Analysis of the worn surfaces of the confirmation experiment revealed adhesive and abrasive wear as the governing mechanisms.
Layer by Layer Mesoporous Silica-Hyaluronic Acid-Cyclodextrin Bifunctional “Lamination”: Study of the Application of Fluorescent Probe and Host–Guest Interactions in the Drug Delivery Field
Authors: Nie Kun , An Qi , Zink Jeffrey I. , Yu Xiang , Zhang Yihe
Published: September 2018

The layer-by-layer technique was exploited to adjust the magnitude of the host–guest interactions between adamantane and cyclodextrin. The effect depends on numerous complex and changeable growth profiles of the films and the number of bilayers. These composite films of mesoporous silica nanoparticles and hyaluronic acid–cyclodextrin(HA-CD) were constructed to load the fluorescent dyes and peptides. The release rates of these molecules would decrease with an increase in the number of layers. A laser scanning confocal microscope was utilized to obtain the diffusion coefficient of fluorescein isothiocyanate. Hybrid films could be applied to increase the loading of different kinds of molecules and could also be integrated into the lamination to delay the rate of release.
Influence of Milling Parameters on Mechanical Properties of AA7075 Aluminum under Corrosion Conditions
Authors: Martín María Jesús , Cano María José , Castillo Germán , Herrera Manuel José , Martín Francisco
Published: September 2018

The paper describes an experimental study developed on the AA7075 T651, which is an aluminum alloy extensively used in the aeronautical industry. This work presents a double approach of investigation where there is no literature about previous research. This includes the analysis of the results obtained by the combination of mechanical and chemical actions on the mechanical properties of this material. On the one hand, the combinations of relevant milling parameters (feed rate, cutting speed) on flat samples (flat specimens have been selected by attempting to reproduce with the most accurate way the geometry and the type of machining process known as face milling is usually used in this manufacturing field). On the other hand, the stimulating effect of the corrosion by salt spray on selected batches of specimens was machined in the previous stage. Results from tensile tests performed on the whole of specimens allowed us to evaluate how the main mechanical properties (yield strength, tensile strength, and elongation at break) have been affected by the processes applied. Elongation at the break presents a reduction in an inverse order to feed a rate increase (up to 24.5%) and this reduction is extended (additional 19.17%) in specimens under corrosion conditions, which results in a greater fragility of the material.
Convergence in Fuzzy Semi-Metric Spaces
Authors: Wu Hsien-Chung
Published: September 2018

The convergence using the fuzzy semi-metric and dual fuzzy semi-metric is studied in this paper. The infimum type of dual fuzzy semi-metric and the supremum type of dual fuzzy semi-metric are proposed in this paper. Based on these two types of dual fuzzy semi-metrics, the different types of triangle inequalities can be obtained. We also study the convergence of these two types of dual fuzzy semi-metrics.
Almost Periodic Solutions of First-Order Ordinary Differential Equations
Authors: Kadry Seifedine , Alferov Gennady , Ivanov Gennady , Sharlay Artem
Published: September 2018

Approaches to estimate the number of almost periodic solutions of ordinary differential equations are considered. Conditions that allow determination for both upper and lower bounds for these solutions are found. The existence and stability of almost periodic problems are studied. The novelty of this paper lies in the fact that the use of apparatus derivatives allows for the reduction of restrictions on the degree of smoothness of the right parts. In our work, regarding the number of periodic solutions of equations first order, we don’t require a high degree of smoothness and no restriction on the smoothness of the second derivative of the Schwartz equation. We have all of these restrictions lifted. Our new form presented also emphasizes this novelty.
Isolation, Characterization, and Pharmaceutical Applications of an Exopolysaccharide from Aerococcus Uriaeequi
Authors: Wang Chunlei , Fan Qiuping , Zhang Xiaofei , Lu Xiaoping , Xu Yanrui , Zhu Wenxing , Zhang Jie , Hao Wen , Hao Lujiang
Published: September 2018

Many marine bacteria secrete exopolysaccharides (EPSs), which are made up of a substantial component of the macro-molecules surrounding cells. Recently, the wide demand for EPSs for food, cosmetics, pharmaceutical and other applications has led to great interest in them. In this study, an EPS produced by marine bacteria Aerococcus uriaeequi HZ strains (EPS-A) was isolated and purified to examine its structure and biological function. The molecular weight of EPS-A analyzed by high-performance liquid gel filtration chromatography (HPGFC) is found to have a number average of 2.22 × 105 and weight average of 2.84 × 105, respectively. High-performance liquid chromatography (HPLC) and Fourier-transform–infrared (FT–IR) analysis indicate that EPS-A was a polysaccharide composed of glucose and a little mannose. In addition, the flocculating rate of sewage of EPS-A was 79.90%. The hygroscopicity studies showed that hygroscopicity of EPS-A was higher than chitosan but lower than that of sodium hyaluronate. The moisture retention of EPS-A showed similar retention activity to both chitosan and sodium hyaluronate. EPS-A also can scavenge free radicals including both OH• free radical and O2•− free radical and the activity to O2•− free radical is similar to vitamin C. Safety assessment on mice indicated that the EPS-A is safe for external use and oral administration. EPS-A has great potential for applications in medicine due to its characteristics mentioned above.
Thermodynamic Aspects in Non-Ideal Metal Membranes for Hydrogen Purification
Authors: Bellini Stefano , Sun Yu , Gallucci Fausto , Caravella Alessio
Published: September 2018

In this paper, an overview on thermodynamic aspects related to hydrogen-metal systems in non-ideal conditions is provided, aiming at systematically merging and analyzing information achieved from several different studies present in the open literature. In particular, the relationships among inner morphology, dissolved hydrogen and internal stresses are discussed in detail, putting in evidence the conformation complexity and the various types of dislocations induced by the presence of H-atoms in the lattice. Specifically, it is highlighted that the octahedral sites are preferentially occupied in the FCC metals (such as palladium), whereas tetrahedral sites are more energetically favored in BCC-structured ones (such as vanadium). These characteristics are shown to lead to a different macroscopic behavior of the two classes of metals, especially in terms of solubility and mechanical failure due to the consequent induced stresses. Furthermore, starting from the expression of the chemical potential generally presented in the literature, a new convenient expression of the activity of the H-atoms dissolved into the metal lattice as a function of the H-concentration is achieved. Such an activity expression is then used in the dissolution equilibrium relationship, which is shown to be the overall result of two different phenomena: (i) dissociative adsorption of molecular hydrogen onto the surface; and (ii) atomic hydrogen dissolution from the surface to the metal bulk. In this way, the obtained expression for equilibrium allows a method to calculate the equilibrium composition in non-ideal conditions (high pressure), which are of interest for real industrial applications.
Continuous Recirculation of Microdroplets in a Closed Loop Tailored for Screening of Bacteria Cultures
Authors: Debski Pawel , Sklodowska Karolina , Michalski Jacek , Korczyk Piotr , Dolata Miroslaw , Jakiela Slawomir
Published: September 2018

Emerging microfluidic technology has introduced new precision controls over reaction conditions. Owing to the small amount of reagents, microfluidics significantly lowers the cost of carrying a single reaction. Moreover, in two-phase systems, each part of a dispersed fluid can be treated as an independent chemical reactor with a volume from femtoliters to microliters, increasing the throughput. In this work, we propose a microfluidic device that provides continuous recirculation of droplets in a closed loop, maintaining low consumption of oil phase, no cross-contamination, stabilized temperature, a constant condition of gas exchange, dynamic feedback control on droplet volume, and a real-time optical characterization of bacterial growth in a droplet. The channels (tubing) and junction cubes are made of Teflon fluorinated ethylene propylene (FEP) to ensure non-wetting conditions and to prevent the formation of biofilm, which is particularly crucial for biological experiments. We show the design and operation of a novel microfluidic loop with the circular motion of microdroplet reactors monitored with optical sensors and precision temperature controls. We have employed the proposed system for long term monitoring of bacterial growth during the antibiotic chloramphenicol treatment. The proposed system can find applications in a broad field of biomedical diagnostics and therapy.
Measurement of Carcinoembryonic Antigen in Clinical Serum Samples Using a Centrifugal Microfluidic Device
Authors: Gao Zhigang , Chen Zongzheng , Deng Jiu , Li Xiaorui , Qu Yueyang , Xu Lingling , Luo Yong , Lu Yao , Liu Tingjiao , Zhao Weijie , Lin Bingcheng
Published: September 2018

Carcinoembryonic antigen (CEA) is a broad-spectrum tumor marker used in clinical applications. The primarily clinical method for measuring CEA is based on chemiluminescence in serum during enzyme-linked immunosorbent assays (ELISA) in 96-well plates. However, this multi-step process requires large and expensive instruments, and takes a long time. In this study, a high-throughput centrifugal microfluidic device was developed for detecting CEA in serum without the need for cumbersome washing steps normally used in immunoreactions. This centrifugal microdevice contains 14 identical pencil-like units, and the CEA molecules are separated from the bulk serum for subsequent immunofluorescence detection using density gradient centrifugation in each unit simultaneously. To determine the optimal conditions for CEA detection in serum, the effects of the density of the medium, rotation speed, and spin duration were investigated. The measured values from 34 clinical serum samples using this high-throughput centrifugal microfluidic device showed good agreement with the known values (average relative error = 9.22%). These results indicate that the high-throughput centrifugal microfluidic device could provide an alternative approach for replacing the classical method for CEA detection in clinical serum samples.
Liquid Cladding Mediated Optical Fiber Sensors for Copper Ion Detection
Authors: Tran Vien Thi , Tran Nhu Hoa Thi , Nguyen Than Thi , Yoon Won Jung , Ju Heongkyu
Published: September 2018

We present a label-free optical fiber based sensor device to detect copper ions (Cu2+) in water. A multimode optical fiber, with its polymer cladding removed along a 1-cm length, is used for the optical sensor head, where the injected Cu2+ in the liquid phase acts as a liquid cladding for the optical mode. The various Cu2+ concentrations modulate the numerical aperture (NA) of the liquid cladding waveguide part. The degree of NA mismatch between the liquid cladding and solid cladding guided parts gives rise to an optical power transmittance change, forming the sensing principle. The presented liquid cladding fiber sensor exhibits a minimum resolvable refractive index of 2.48 × 10−6. For Cu2+ detection, we functionalize the sensor head surface (fiber core) using chitosan conjugated ethylenediaminetetraacetic acid (EDTA) which captures Cu2+ effectively due to the enhanced chelating effects. We obtain a limit of detection of Cu2+ of 1.62 nM (104 ppt), which is significantly lower than the tolerable level in drinking water (~30 µM), and achieve a dynamic range of 1 mM. The simple structure of the sensor head and the sensing system ensures the potential capability of being miniaturized. This may allow for in-situ, highly-sensitive, heavy metal sensors in a compact format.
LED Optrode with Integrated Temperature Sensing for Optogenetics
Authors: Goncalves S. Beatriz , Palha José M. , Fernandes Helena C. , Souto Márcio R. , Pimenta Sara , Dong Tao , Yang Zhaochu , Ribeiro João F. , Correia José H.
Published: September 2018

In optogenetic studies, the brain is exposed to high-power light sources and inadequate power density or exposure time can cause cell damage from overheating (typically temperature increasing of 2 ∘ C). In order to overcome overheating issues in optogenetics, this paper presents a neural tool capable of assessing tissue temperature over time, combined with the capability of electrical recording and optical stimulation. A silicon-based 8 mm long probe was manufactured to reach deep neural structures. The final proof-of-concept device comprises a double-sided function: on one side, an optrode with LED-based stimulation and platinum (Pt) recording points; and, on the opposite side, a Pt-based thin-film thermoresistance (RTD) for temperature assessing in the photostimulation site surroundings. Pt thin-films for tissue interface were chosen due to its biocompatibility and thermal linearity. A single-shaft probe is demonstrated for integration in a 3D probe array. A 3D probe array will reduce the distance between the thermal sensor and the heating source. Results show good recording and optical features, with average impedance magnitude of 371 k Ω , at 1 kHz, and optical power of 1.2 mW·mm − 2 (at 470 nm), respectively. The manufactured RTD showed resolution of 0.2 ∘ C at 37 ∘ C (normal body temperature). Overall, the results show a device capable of meeting the requirements of a neural interface for recording/stimulating of neural activity and monitoring temperature profile of the photostimulation site surroundings, which suggests a promising tool for neuroscience research filed.
Rhizophagy Cycle: An Oxidative Process in Plants for Nutrient Extraction from Symbiotic Microbes
Authors: White James F. , Kingsley Kathryn L. , Verma Satish K. , Kowalski Kurt P.
Published: September 2018

In this paper, we describe a mechanism for the transfer of nutrients from symbiotic microbes (bacteria and fungi) to host plant roots that we term the ‘rhizophagy cycle.’ In the rhizophagy cycle, microbes alternate between a root intracellular endophytic phase and a free-living soil phase. Microbes acquire soil nutrients in the free-living soil phase; nutrients are extracted through exposure to host-produced reactive oxygen in the intracellular endophytic phase. We conducted experiments on several seed-vectored microbes in several host species. We found that initially the symbiotic microbes grow on the rhizoplane in the exudate zone adjacent the root meristem. Microbes enter root tip meristem cells—locating within the periplasmic spaces between cell wall and plasma membrane. In the periplasmic spaces of root cells, microbes convert to wall-less protoplast forms. As root cells mature, microbes continue to be subjected to reactive oxygen (superoxide) produced by NADPH oxidases (NOX) on the root cell plasma membranes. Reactive oxygen degrades some of the intracellular microbes, also likely inducing electrolyte leakage from microbes—effectively extracting nutrients from microbes. Surviving bacteria in root epidermal cells trigger root hair elongation and as hairs elongate bacteria exit at the hair tips, reforming cell walls and cell shapes as microbes emerge into the rhizosphere where they may obtain additional nutrients. Precisely what nutrients are transferred through rhizophagy or how important this process is for nutrient acquisition is still unknown.
Beneficial Effects of Tomato Juice Fermented by Lactobacillus Plantarum and Lactobacillus Casei: Antioxidation, Antimicrobial Effect, and Volatile Profiles
Authors: Liu Yiyun , Chen Haiming , Chen Wenxue , Zhong Quipping , Zhang Guanfei , Chen Weijun
Published: September 2018

Tomato juice was fermented by Lactobacillus plantarum and Lactobacillus casei to produce an innovative high-bioactivity probiotic beverage. The levels of lycopene, total carotenoids, ascorbic acid, total phenolic and volatile compounds, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azinobis-3-ethylbenzotiazo-line-6-sulfonic acid (ABTS) radical scavenging capacities, ferric reducing antioxidant power (FRAP), and Escherichia coli flora, as well as the inhibition of copper-induced human low-density lipoproteins (LDL)-cholesterol oxidation assays, were measured. The results revealed that the ABTS and DPPH inhibition values, as well as the FRAP and total phenolic content, were significantly increased. LDL-cholesterol oxidation was markedly delayed after the addition of the fermented juice. The in vitro inhibitory effects of Escherichia coli flora were substantially increased after being fermented with Lactobacillus plantarum and Lactobacillus casei. The results associated with the volatile compounds indicated that fermentation with Lactobacillus plantarum and Lactobacillus casei is a meaningful strategy for modifying flavors.
RhoA/ROCK-2 Pathway Inhibition and Tight Junction Protein Upregulation by Catalpol Suppresses Lipopolysaccaride-Induced Disruption of Blood-Brain Barrier Permeability
Authors: Feng Shan , Zou Li , Wang Hongjin , He Ran , Liu Ke , Zhu Huifeng
Published: September 2018

Lipopolysaccaride (LPS) directly or indirectly injures brain microvascular endothelial cells (BMECs) and damages the intercellular tight junction that gives rise to altered blood-brain barrier (BBB) permeability. Catalpol plays a protective role in LPS-induced injury, but whether catalpol protects against LPS-caused damage of BBB permeability and the underlying mechanism remain to be delineated. Prophylactic protection with catalpol (5 mg/kg, i.v.) consecutively for three days reversed the LPS-induced damage of BBB by decreased Evans Blue (EB) leakage and restored tight junctions in C57 mice. Besides, catalpol co-administrated with LPS increased BMECs survival, decreased their endothelin-1, TNF-Α and IL-6 secretion, improved transmembrane electrical resistance in a time-dependent manner, and in addition increased the fluorescein sodium permeability coefficient of BMECs. Also, transmission electron microscopy showed catalpol protective effects on tight junctions. Fluorescence staining displayed that catalpol reversed the rearrangement of the cytoskeleton protein F-actin and upregulated the tight junction protein of claudin-5 and ZO-1, which have been further demonstrated by the mRNA and protein expression levels of ZO-1, ZO-2, ZO-3, claudin-5, and occludin. Moreover, catalpol concurrently downregulated the mRNA and protein levels of RhoA, and ROCK2, the critical proteins in the RhoA/ROCK2 signaling pathway. This study thus indicated that catalpol, via inhibition of the RhoA/ROCK2 signaling pathway, reverses the disaggregation of cytoskeleton actin in BMECs and prevents down-regulation of junctional proteins, such as claudin-5, occludin, and ZO-1, and decreases endothelin-1 and inflammatory cytokine secretion, eventually alleviating the increase in LPS-induced BBB permeability.
Aggregation-Induced Emission of Tetraphenylethene-Conjugated Phenanthrene Derivatives and Their Bio-Imaging Applications
Authors: Khuong Mai Duy , Lee Joomin , Min Ilgi , Vales Temmy Pegarro , Choi Kyong-Hoon , Park Bong Joo , Cho Sung , Kim Ho-Joong
Published: September 2018

In this study, a series of rationally designed emissive phenanthrene derivatives were synthesized and their aggregation-induced emission (AIE) properties in tetrahydrofuran (THF)/water mixtures were investigated. Two tetraphenylethene (TPE) segments were conjugated to both ends of the phenanthrene core at the para-positions and meta-positions, resulting in pTPEP and mTPEP derivatives, respectively. While the TPE-conjugated phenanthrene derivatives did not show any emission when dissolved in pure THF, they showed strong sky-blue emissions in water-THF mixtures, which is attributed to the restriction of intramolecular motions of TPE segments by aggregation. Furthermore, silica nanoparticles loaded with these AIE-active compounds were prepared and proved to be promising intracellular imaging agents.
Microstructure and Mechanical Properties of Nano-Carbon Reinforced Titanium Matrix/Hydroxyapatite Biocomposites Prepared by Spark Plasma Sintering
Authors: Li Feng , Jiang Xiaosong , Shao Zhenyi , Zhu Degui , Luo Zhiping
Published: September 2018

Nano-carbon reinforced titanium matrix/hydroxyapatite (HA) biocomposites were successfully prepared by spark plasma sintering (SPS). The microstructure, mechanical properties, biocompatibility, and the relationship between microstructure and properties of biocomposites were systematically investigated. Results showed there are some new phases in sintered composites, such as β-Ti, TiO3, ZrO2, etc. Moreover, a small amount of Ti17P10, CaTiO3, Ca3(PO4)2 were also detected. The reaction that may occur during the preparation process is suppressed to some extent, which is because that the addition of second phases can prevent the direct contact of titanium with HA and reduce the contact areas. Transmission electron microscope (TEM) analysis proved the existence of elemental diffusion and chemical reactions in sintered composites. Compared with results of composites prepared by hot-pressed sintering before, mechanical properties (microhardness, compressive strength, and shear strength) of 0.5-GNFs composites prepared by SPS were increased by about 2.8, 4.8, and 4.1 times, respectively. The better mechanical properties of 0.5-GNFs composite in nano-carbon reinforced composites are mainly due to the lower degree of agglomeration of tubular carbon nanotubes (CNTs) compared to lamellar graphene nanoflakes (GNFs). Moreover, the strengthening and toughening mechanisms of nano-carbon reinforced titanium alloy/HA biocomposite prepared by spark plasma sintering (SPS) mainly included second phase strengthening, grain refinement strengthening, solution strengthening, graphene extraction, carbon nanotubes bridging, crack tail stripping, etc. In addition, in vitro bioactivity test revealed that the addition of nano-carbon was beneficial to promote the adhesion and proliferation of cells on the surface of titanium alloy/HA composite, because nano-carbon can enhance the formation of mineralized necks in the composites after transplantation, stimulate biomineralization and promote bone regeneration.
The Enhanced Lithium-Storage Performance for MnO Nanoparticles Anchored on Electrospun Nitrogen-Doped Carbon Fibers
Authors: Zhang Rui , Dong Xue , Peng Lechao , Kang Wenjun , Li Haibo
Published: September 2018

Manganese monoxide (MnO) is a promising anode material in the lithium-ion battery for its high capacity, low operation potential, and environmental benignity. However, its application is impeded by poor rate capability and rapid capacity fading. In this work, a MnO/carbon hybrid material, in which small-sized MnO nanoparticles are tightly anchored on carbon fibers (denoted as MnO@CFs), was prepared by annealing the electrospun precursor fibers at 650 °C. When applied as the anode material of the Li-ion battery, the small size of MnO shortens the Li-ion diffusion path, and the carbon fibers not only greatly improve the conductivity but also efficiently buffer the MnO structure strain during the charge–discharge process, endowing the MnO@CFs electrode with a good rate capability (185 mAh g−1 at 5 A g−1) and cyclic stability (406 mAh g−1 after 500 cycles at 1.0 A g−1).
Electrospinning Fabrication of Poly(vinyl alcohol)/Coptis chinensis Extract Nanofibers for Antimicrobial Exploits
Authors: Yang Seong Baek , Kim Eun Hee , Kim Seung Hee , Kim Young Hun , Oh Weontae , Lee Jin-Tae , Jang Young-Ah , Sabina Yeasmin , Ji Byung Chul , Yeum Jeong Hyun
Published: September 2018

Coptis chinensis (CC) is used in conventional Chinese medicine. The main active components of CC are isoquinoline alkaloids, including berberine, coptisine, palmatine, and magnoflorine; all these are known to have several pharmacological properties. Poly(vinyl alcohol) (PVA) is a well-known synthetic biocompatible polymer suitable for a range of pharmaceutical uses; it can be used as a matrix for the incorporation of functional materials and has a wide range of applications in the cosmetics, food, pharmaceutical, and packaging industries. In this study, PVA-based electrospun nanofibers containing CC extract were successfully fabricated. Furthermore, the effects of different CC extract contents on the morphologies, and antimicrobial and antifungal properties of PVA/CC extract nanofibers were investigated. Morphological changes were observed using different molecular weights of PVA. For characterization, field-emission scanning electron microscopy, thermogravimetric analysis, and Fourier transform infrared analysis were performed. The effectiveness of these nanofibers has been demonstrated by evaluating the thermal stability against Staphylococcus aureus, antimicrobial activity against Staphylococcus aureus and Staphylococcus epidermidis, and the antifungal activity against the fungi Aureobasidium pullulans and Penicillium pinophilum. The PVA/CC extract nanofibers were found to have excellent antibacterial and antifungal activity and thermal stability; hence, their use in medicinal sectors is highly recommended.
Novel Roles of Non-Coding RNAs in Opioid Signaling and Cardioprotection
Authors: Melo Zesergio , Ishida Cecilia , Goldaraz Maria de la Paz , Rojo Rocio , Echavarria Raquel
Published: September 2018

Cardiovascular disease (CVD) is a significant cause of morbidity and mortality across the world. A large proportion of CVD deaths are secondary to coronary artery disease (CAD) and myocardial infarction (MI). Even though prevention is the best strategy to reduce risk factors associated with MI, the use of cardioprotective interventions aimed at improving patient outcomes is of great interest. Opioid conditioning has been shown to be effective in reducing myocardial ischemia-reperfusion injury (IRI) and cardiomyocyte death. However, the molecular mechanisms behind these effects are under investigation and could provide the basis for the development of novel therapeutic approaches in the treatment of CVD. Non-coding RNAs (ncRNAs), which are functional RNA molecules that do not translate into proteins, are critical modulators of cardiac gene expression during heart development and disease. Moreover, ncRNAs such as microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) are known to be induced by opioid receptor activation and regulate opioid signaling pathways. Recent advances in experimental and computational tools have accelerated the discovery and functional characterization of ncRNAs. In this study, we review the current understanding of the role of ncRNAs in opioid signaling and opioid-induced cardioprotection.
Paediatric Home Artificial Nutrition in Italy: Report from 2016 Survey on Behalf of Artificial Nutrition Network of Italian Society for Gastroenterology, Hepatology and Nutrition (SIGENP)
Authors: Lezo Antonella , Capriati Teresa , Spagnuolo Maria Immacolata , Lacitignola Laura , Goreva Irina , Di Leo Grazia , Cecchi Nicola , Gandullia Paolo , Amarri Sergio , Forchielli Maria Luisa , Dipasquale Valeria , Parma Barbara , Gatti Simona , Ravaioli Elisa , Salvatore Silvia , Mainetti Martina , Norsa Lorenzo , Pellegrino Maristella , Fornaro Martina , Fiorito Valentina , Lanari Marcello , Giaquinto Ester , Verduci Elvira , Baldassarre Maria Elisabetta , Diamanti Antonella
Published: September 2018

Home Artificial Nutrition (HAN) is a safe and efficacious technique that insures children’s reintegration into the family, society and school. Epidemiological data on paediatric HAN in Italy are not available. Aim: to detect the prevalence and incidence of Home Parenteral Nutrition (HPN) and Home Enteral Nutrition (HEN), either via tube or mouth, in Italy in 2016. Materials and methods: a specific form was sent to all registered SIGENP members and investigators of local HAN centres, inviting them to provide the requested centre’s data and demographics, underlying diseases and HAN characteristics of the patients. Results: we recorded 3403 Italian patients on HAN aged 0 to 19 years from 22 centres: 2277 HEN, 950 Oral Nutritional Supplements (ONS) and 179 HPN programs. The prevalence of HEN (205 pts/million inhabitants) and HPN (16 pts/million inhabitants) has dramatically increased in Italy in the last 9 years. Neurodisabling conditions were the first indication for HEN by tube or mouth while HPN is mainly requested in digestive disorders. Conclusions: HAN is a widespread and rapidly growing treatment in Italy, as well as in other European countries. Awareness of its extent and characteristics helps improving HAN service and patients’ quality of life.