bluuebunny/crossref_metadata_2025 huggingface dataset:
Autologous cell transplantation for treatment of colorectal aganglionosis in mice
Authors: Pan Weikang , Rahman Ahmed A. , Ohkura Takahiro , Stavely Rhian , Ohishi Kensuke , Han Christopher Y. , Leavitt Abigail , Kashiwagi Aki , Burns Alan J. , Goldstein Allan M. , Hotta Ryo
Published: March 2024

Neurointestinal diseases cause significant morbidity and effective treatments are lacking. This study aimes to test the feasibility of transplanting autologous enteric neural stem cells (ENSCs) to rescue the enteric nervous system (ENS) in a model of colonic aganglionosis. ENSCs are isolated from a segment of small intestine from Wnt1::Cre;R26iDTR mice in which focal colonic aganglionosis is simultaneously created by diphtheria toxin injection. Autologous ENSCs are isolated, expanded, labeled with lentiviral-GFP, and transplanted into the aganglionic segment in vivo. ENSCs differentiate into neurons and glia, cluster to form neo-ganglia, and restore colonic contractile activity as shown by electrical field stimulation and optogenetics. Using a non-lethal model of colonic aganglionosis, our results demonstrate the potential of autologous ENSC therapy to improve functional outcomes in neurointestinal disease, laying the groundwork for clinical application of this regenerative cell-based approach.
Locking the lattice oxygen in RuO2 to stabilize highly active Ru sites in acidic water oxidation
Authors: Ping Xinyu , Liu Yongduo , Zheng Lixia , Song Yang , Guo Lin , Chen Siguo , Wei Zidong
Published: March 2024

Ruthenium dioxide is presently the most active catalyst for the oxygen evolution reaction (OER) in acidic media but suffers from severe Ru dissolution resulting from the high covalency of Ru-O bonds triggering lattice oxygen oxidation. Here, we report an interstitial silicon-doping strategy to stabilize the highly active Ru sites of RuO2 while suppressing lattice oxygen oxidation. The representative Si-RuO2−0.1 catalyst exhibits high activity and stability in acid with a negligible degradation rate of ~52 μV h−1 in an 800 h test and an overpotential of 226 mV at 10 mA cm−2. Differential electrochemical mass spectrometry (DEMS) results demonstrate that the lattice oxygen oxidation pathway of the Si-RuO2−0.1 was suppressed by ∼95% compared to that of commercial RuO2, which is highly responsible for the extraordinary stability. This work supplied a unique mentality to guide future developments on Ru-based oxide catalysts’ stability in an acidic environment.
Dissecting the mechanism of atlastin-mediated homotypic membrane fusion at the single-molecule level
Authors: Shi Lijun , Yang Chenguang , Zhang Mingyuan , Li Kangning , Wang Keying , Jiao Li , Liu Ruming , Wang Yunyun , Li Ming , Wang Yong , Ma Lu , Hu Shuxin , Bian Xin
Published: March 2024

Homotypic membrane fusion of the endoplasmic reticulum (ER) is mediated by dynamin-like GTPase atlastin (ATL). This fundamental process relies on GTP-dependent domain rearrangements in the N-terminal region of ATL (ATLcyto), including the GTPase domain and three-helix bundle (3HB). However, its conformational dynamics during the GTPase cycle remain elusive. Here, we combine single-molecule FRET imaging and molecular dynamics simulations to address this conundrum. Different from the prevailing model, ATLcytocan form a loose crossover dimer upon GTP binding, which is tightened by GTP hydrolysis for membrane fusion. Furthermore, the α-helical motif between the 3HB and transmembrane domain, which is embedded in the surface of the lipid bilayer and self-associates in the crossover dimer, is required for ATL function. To recycle the proteins, Pi release, which disassembles the dimer, activates frequent relative movements between the GTPase domain and 3HB, and subsequent GDP dissociation alters the conformational preference of the ATLcytomonomer for entering the next reaction cycle. Finally, we found that two disease-causing mutations affect human ATL1 activity by destabilizing GTP binding-induced loose crossover dimer formation and the membrane-embedded helix, respectively. These results provide insights into ATL-mediated homotypic membrane fusion and the pathological mechanisms of related disease.
Benchmarking highly entangled states on a 60-atom analogue quantum simulator
Authors: Shaw Adam L. , Chen Zhuo , Choi Joonhee , Mark Daniel K. , Scholl Pascal , Finkelstein Ran , Elben Andreas , Choi Soonwon , Endres Manuel
Published: March 2024

Quantum systems have entered a competitive regime in which classical computers must make approximations to represent highly entangled quantum states1,2. However, in this beyond-classically-exact regime, fidelity comparisons between quantum and classical systems have so far been limited to digital quantum devices2–5, and it remains unsolved how to estimate the actual entanglement content of experiments6. Here, we perform fidelity benchmarking and mixed-state entanglement estimation with a 60-atom analogue Rydberg quantum simulator, reaching a high-entanglement entropy regime in which exact classical simulation becomes impractical. Our benchmarking protocol involves extrapolation from comparisons against an approximate classical algorithm, introduced here, with varying entanglement limits. We then develop and demonstrate an estimator of the experimental mixed-state entanglement6, finding our experiment is competitive with state-of-the-art digital quantum devices performing random circuit evolution2–5. Finally, we compare the experimental fidelity against that achieved by various approximate classical algorithms, and find that only the algorithm we introduce is able to keep pace with the experiment on the classical hardware we use. Our results enable a new model for evaluating the ability of both analogue and digital quantum devices to generate entanglement in the beyond-classically-exact regime, and highlight the evolving divide between quantum and classical systems.
Transcription–replication conflicts underlie sensitivity to PARP inhibitors
Authors: Petropoulos Michalis , Karamichali Angeliki , Rossetti Giacomo G. , Freudenmann Alena , Iacovino Luca G. , Dionellis Vasilis S. , Sotiriou Sotirios K. , Halazonetis Thanos D.
Published: March 2024

An important advance in cancer therapy has been the development of poly(ADP-ribose) polymerase (PARP) inhibitors for the treatment of homologous recombination (HR)-deficient cancers1–6. PARP inhibitors trap PARPs on DNA. The trapped PARPs are thought to block replisome progression, leading to formation of DNA double-strand breaks that require HR for repair7. Here we show that PARP1 functions together with TIMELESS and TIPIN to protect the replisome in early S phase from transcription–replication conflicts. Furthermore, the synthetic lethality of PARP inhibitors with HR deficiency is due to an inability to repair DNA damage caused by transcription–replication conflicts, rather than by trapped PARPs. Along these lines, inhibiting transcription elongation in early S phase rendered HR-deficient cells resistant to PARP inhibitors and depleting PARP1 by small-interfering RNA was synthetic lethal with HR deficiency. Thus, inhibiting PARP1 enzymatic activity may suffice for treatment efficacy in HR-deficient settings.
SQANTI3: curation of long-read transcriptomes for accurate identification of known and novel isoforms
Authors: Pardo-Palacios Francisco J. , Arzalluz-Luque Angeles , Kondratova Liudmyla , Salguero Pedro , Mestre-Tomás Jorge , Amorín Rocío , Estevan-Morió Eva , Liu Tianyuan , Nanni Adalena , McIntyre Lauren , Tseng Elizabeth , Conesa Ana
Published: March 2024

SQANTI3 is a tool designed for the quality control, curation and annotation of long-read transcript models obtained with third-generation sequencing technologies. Leveraging its annotation framework, SQANTI3 calculates quality descriptors of transcript models, junctions and transcript ends. With this information, potential artifacts can be identified and replaced with reliable sequences. Furthermore, the integrated functional annotation feature enables subsequent functional iso-transcriptomics analyses.
MiRNA-296-5p promotes the sensitivity of nasopharyngeal carcinoma cells to cisplatin via targeted inhibition of STAT3/KLF4 signaling axis
Authors: Luo Hai-qing , Wang Yan , Ren Jing , Zhang Quan-ying , Chen Yan , Chen Mei-hui , Huang Ning-xin , Wu Min-hua , Tang Xu-dong , Li Xiang-yong
Published: March 2024

Improving drug sensitivity is an important strategy in chemotherapy of cancer and accumulating evidence indicates that miRNAs are involved in the regulation of drug sensitivity, but the specific mechanism is still unclear. Our previous study has found that miR-296-5p was significantly downregulated in nasopharyngeal carcinoma (NPC). Here, we aim to explore whether miR-296-5p is involved in regulating cisplatin sensitivity in NPC by regulating STAT3/KLF4 signaling axis. The cell proliferation and clonogenic capacity of NPC cells were evaluated by CCK8 Assay and plate colony assay, respectively. The Annexin V-FITC staining kit was used to determine and quantify the apoptotic cells using flow cytometry. The drug efflux ability of NPC cells were determined by Rhodamine 123 efflux experiment. The expression of miR-296-5p, apoptosis-related genes and protein in NPC cell lines were detected by qPCR and Western blot, respectively. Animal study was used to evaluate the sensitivity of NPC cells to DDP treatment in vivo. Our results showed that elevated miR-296-5p expression obviously promoted the sensitivity of NPC cells to DDP by inhibiting cell proliferation and clonogenic capacity, and inducing apoptosis. In addition, we found that miR-296-5p inhibited the expression of STAT3 and KLF4 in NPC cells, while overexpression of exogenous STAT3 reversed miR-296-5p-mediated enhancement in cell death of DDP-treated NPC cells. In vivo studies further confirmed that miR-296-5p promotes the sensitivity of NPC cells to DDP treatment. miRNA-296-5p enhances the drug sensitivity of nasopharyngeal carcinoma cells to cisplatin via STAT3/KLF4 signaling pathway.
Sex-specific associations of empirically derived dietary patterns with colorectal cancer risk in a Korean population: a case‒control study
Authors: Kim Minji , Gunathilake Madhawa , Lee Jeonghee , Oh Jae Hwan , Chang Hee Jin , Sohn Dae Kyung , Shin Aesun , Kim Jeongseon
Published: March 2024

Dietary patterns may be a crucial modifiable factor in colorectal cancer (CRC) risk. This study aimed to examine the associations of dietary patterns derived from two methods with CRC risk in Korea. In a study of 1420 CRC patients and 2840 control participants, we obtained dietary patterns by principal component analysis (PCA) and reduced rank regression (RRR) using 33 predefined food groups. The associations between dietary patterns and CRC risk were assessed using unconditional logistic regression models to calculate odds ratios (ORs) and 95% confidence intervals (CIs). We identified two similar dietary patterns, derived from PCA 1 (prudent) and RRR (healthy), characterized by higher consumption of green/yellow vegetables, light-colored vegetables, fruits, eggs, and milk in both men and women. In women, higher prudent and healthy pattern scores were significantly associated with a lower risk of CRC (prudent, ORQ4 vs. Q1 = 0.59, 95% CI 0.40–0.86, P for trend = 0.005; healthy, ORQ4 vs. Q1 = 0.62, 95% CI 0.43–0.89, P for trend = 0.007). In men, a significant inverse association between dietary pattern and risk of rectal cancer was found only for the healthy dietary pattern (ORQ4 vs. Q1 = 0.66, 95% CI 0.45–0.97, P for trend = 0.036). Compared with the dietary pattern derived by PCA, the RRR dietary pattern had a slightly stronger association with a lower risk of distal colon cancer (ORQ4 vs. Q1 = 0.58, 95% CI 0.35–0.97, P for trend = 0.025) and rectal cancer (ORQ4 vs. Q1 = 0.29, 95% CI 0.15–0.57, P for trend < 0.001) in women. Our findings suggest cancer prevention strategies focusing on a diet rich in vegetables, fruits, eggs, and milk. Moreover, the use of both PCA and RRR methods may be advantageous to explore the associations between dietary patterns and risk of CRC.
Electrospun network based on polyacrylonitrile-polyphenyl/titanium oxide nanofibers for high-performance supercapacitor device
Authors: Kenawy El-Refaie , Moharram Youssef I. , Abouharga Fatma S. , Elfiky Mona
Published: March 2024

Nanofibers and mat-like polyacrylonitrile-polyphenyl/titanium oxide (PAN-Pph./TiO2) with proper electrochemical properties were fabricated via a single-step electrospinning technique for supercapacitor application. Scanning electron microscopy (SEM), scanning transmission electron microscopy (STEM), thermogravimetry (TGA), fourier transform infrared (FTIR), X-ray diffraction (XRD) and energy dispersive X-ray (EDX) were conducted to characterize the morphological and chemical composition of all fabricated nanofibers. Furthermore, the electrochemical activity of the fabricated nanofibers for energy storage applications (supercapacitor) was probed by cyclic voltammetry (CV), charge–discharge (CD), and electrochemical impedance spectroscopy (EIS). The PAN-PPh./TiO2 nanofiber electrode revealed a proper specific capacitance of 484 F g−1 at a current density of 11.0 A g–1 compared with PAN (198 F g−1), and PAN-PPh. (352 F g−1) nanofibers using the charge–discharge technique. Furthermore, the PAN-PPh./TiO2 nanofiber electrode displayed a proper energy density of 16.8 Wh kg−1 at a power density (P) of 2749.1 Wkg−1. Moreover, the PAN-PPh./TiO2 nanofiber electrode has a low electrical resistance of 23.72 Ω, and outstanding cycling stability of 79.38% capacitance retention after 3000 cycles.
Open-label placebos enhance test performance and reduce anxiety in learner drivers: a randomized controlled trial
Authors: Schaefer Michael , Enge Sören
Published: March 2024

Passing the driving school test can be very challenging, especially in big cities, where up to 52% of all students fail this test. Consequently, many learner drivers experience stress and anxiety. For some learner drivers these feelings can be extreme and negatively affect the performance in the driving test. Different strategies to face anxiety and stress are known, including, for example, psychological or pharmacological approaches and even placebo pills. Recent intriguing findings have also demonstrated that placebos without deception, so-called open-label placebos, successfully reduce anxiety. Here we aimed to test effects of this novel treatment for learner drivers. We investigated whether open-label placebos affect test performance and feelings of anxiety in learner drivers. Sixty-eight healthy participants (mean age 21.94 years, 26 females) were randomized into two groups. The open-label placebo group received placebo pills two weeks before the driving test (two pills each day). The control group received no treatment. Results revealed that the open-label placebo group experienced significantly less anxiety than the control group before the test (measured with the State-Trait-Anxiety-Inventory, STAI-S, and the German Test Anxiety Inventory, PAF). Moreover, in the open-label placebo group less learner drivers failed the driving test (29.41% vs. 52.95%). The results suggest that open-label placebos may provide an ethical unproblematic way to experience less anxiety and might also enhance the probability to pass the driving test. We discuss possible mechanisms of open-label placebos and limitations of our findings.
The E3 ligase TRIM7 suppresses the tumorigenesis of gastric cancer by targeting SLC7A11
Authors: Chen Qishuai , Zhang Tongtong , Zeng Runzhi , Zhang Kunmiao , Li Bingjun , Zhu Zhenguo , Ma Xiaomin , Zhang Yun , Li Linchuan , Zhu Jiankang , Zhang Guangyong
Published: March 2024

Tripartite motif-containing protein 7 (TRIM7), as an E3 ligase, plays an important regulatory role in various physiological and pathological processes. However, the role of TRIM7 in gastric cancer (GC) is still undefined. Our study detected the expression of TRIM7 in clinical specimens and investigated the regulatory effect and molecular mechanism of TRIM7 on GC progression through in vitro and in vivo experiments. Our finding showed that TRIM7 was significantly downregulated in GC, and patients with high expression of TRIM7 showed long overall survival. Both in vitro and in vivo experiments showed that TRIM7 dramatically suppressed the malignant progression of GC. Further investigation showed that ferroptosis was the major death type mediated by TRIM7. Mechanistically, TRIM7 interacted with SLC7A11 through its B30.2/SPRY domain and promoted Lys48-linked polyubiquitination of SLC7A11, which effectively suppressing SLC7A11/GPX4 axis and inducing ferroptosis in GC cells. In vivo experiments and correlation analysis based on clinical specimens further confirmed that TRIM7 inhibited tumor growth through suppressing SLC7A11/GPX4 axis. In conclusion, our investigation demonstrated for the first time that TRIM7, as a tumor suppressor, induced ferroptosis via targeting SLC7A11 in GC, which provided a new strategy for the molecular therapy of GC by upregulating TRIM7.
Impact of seed priming with Selenium nanoparticles on germination and seedlings growth of tomato
Authors: García-Locascio Ezequiel , Valenzuela Edgardo I. , Cervantes-Avilés Pabel
Published: March 2024

Poor germination and seedlings growth can lead to significant economic losses for farmers, therefore, sustainable agricultural strategies to improve germination and early growth of crops are urgently needed. The objective of this work was to evaluate selenium nanoparticles (Se NPs) as nanopriming agents for tomato (Solanum lycopersicum) seeds germinated without stress conditions in both trays and Petri dishes. Germination quality, seedlings growth, synergism-antagonism of Se with other elements, and fate of Se NPs, were determined as function of different Se NPs concentrations (1, 10 and 50 ppm). Results indicated that the germination rate in Petri dishes improved with 10 ppm, while germination trays presented the best results at 1 ppm, increasing by 10 and 32.5%, respectively. Therefore, seedlings growth was measured only in germination trays. Proline content decreased up to 22.19% with 10 ppm, while for same treatment, the total antioxidant capacity (TAC) and total chlorophyll content increased up to 38.97% and 21.28%, respectively. Antagonisms between Se with Mg, K, Mn, Zn, Fe, Cu and Mo in the seed were confirmed. In the case of seedlings, the N content decreased as the Se content increased. Transmission Electron Microscopy (TEM) imaging confirmed that Se NPs surrounded the plastids of the seed cells. By this finding, it can be inferred that Se NPs can reach the embryo, which is supported by the antagonism of Se with important nutrients involved in embryogenesis, such as K, Mg and Fe, and resulted in a better germination quality. Moreover, the positive effect of Se NPs on total chlorophyll and TAC, and the negative correlation with proline content with Se content in the seed, can be explained by Se NPs interactions with proplastids and other organelles within the cells, resulting with the highest length and fresh weight when seeds were exposed to 1 ppm.
Enhancing the driving experience of smart city users based on content delivery framework for intelligent transportation systems
Authors: Alqubaysi Tariq , Yousef Amr
Published: March 2024

Integration of several communication technologies that facilitate user access contributes to the rapid development of the smart city notion. Intelligent transportation systems (ITS) are introduced as part of smart city development to provide drivers with enhanced communication and information-sharing capabilities. The article introduces a novel ITS content delivery framework (CDF) that addresses communication outage issues. CDF-ITS uses End-to-end decision-making system modelling to examine factors such as communication, content distribution, and vehicle features. A suitable communication slot for vehicular users is determined by processing these characteristics based on outage time and variables. By allocating time-aware communication slots according to the classification of the propagation factor, outage problems may be reduced. End-to-end decision-making is used for classification and vehicle attribute balance, allowing immediate responses to user requests. The experimental outcomes show that the latency of 0.297 s, outage time of 0.0837, distributed messages of 276, and computing complexity of 11.87 are used to assess the proposed framework's efficiency across vehicle density and velocities.
Hybridization of graphene-gold plasmons for active control of mid-infrared radiation
Authors: Feinstein Matthew D. , Almeida Euclides
Published: March 2024

Many applications in environmental and biological sensing, standoff detection, and astronomy rely on devices that operate in the mid-infrared range, where active devices can play a critical role in advancing discovery and innovation. Nanostructured graphene has been proposed for active miniaturized mid-infrared devices via excitation of tunable surface plasmons, but typically present low efficiencies due to weak coupling with free-space radiation and plasmon damping. Here we present a strategy to enhance the light-graphene coupling efficiency, in which graphene plasmons couple with gold localized plasmons, creating novel hybridized plasmonic modes. We demonstrate a metasurface in which hybrid plasmons are excited with transmission modulation rates of 17% under moderate doping (0.35 eV) and in ambient conditions. We also evaluate the metasurface as a mid-infrared modulator, measuring switching speeds of up to 16 kHz. Finally, we propose a scheme in which we can excite strongly coupled gold-graphene gap plasmons in the thermal radiation range, with applications to nonlinear optics, slow light, and sensing.
Erythropoietin receptor signal is crucial for periodontal ligament stem cell-based tissue reconstruction in periodontal disease
Authors: Zakaria MHD. Fouad , Sonoda Soichiro , Kato Hiroki , Ma Lan , Uehara Norihisa , Kyumoto-Nakamura Yukari , Sharifa M. Majd , Yu Liting , Dai Lisha , Yamauchi-Tomoda Erika , Aijima Reona , Yamaza Haruyoshi , Nishimura Fusanori , Yamaza Takayoshi
Published: March 2024

Alveolar bone loss caused by periodontal disease eventually leads to tooth loss. Periodontal ligament stem cells (PDLSCs) are the tissue-specific cells for maintaining and repairing the periodontal ligament, cementum, and alveolar bone. Here, we investigated the role of erythropoietin receptor (EPOR), which regulates the microenvironment-modulating function of mesenchymal stem cells, in PDLSC-based periodontal therapy. We isolated PDLSCs from patients with chronic periodontal disease and healthy donors, referred to as PD-PDLSCs and Cont-PDLSCs, respectively. PD-PDLSCs exhibited reduced potency of periodontal tissue regeneration and lower expression of EPOR compared to Cont-PDLSCs. EPOR-silencing suppressed the potency of Cont-PDLSCs mimicking PD-PDLSCs, whereas EPO-mediated EPOR activation rejuvenated the reduced potency of PD-PDLSCs. Furthermore, we locally transplanted EPOR-silenced and EPOR-activated PDLSCs into the gingiva around the teeth of ligament-induced periodontitis model mice and demonstrated that EPOR in PDLSCs participated in the regeneration of the periodontal ligament, cementum, and alveolar bone in the ligated teeth. The EPOR-mediated paracrine function of PDLSCs maintains periodontal immune suppression and bone metabolic balance via osteoclasts and osteoblasts in the periodontitis model mice. Taken together, these results suggest that EPOR signaling is crucial for PDLSC-based periodontal regeneration and paves the way for the development of novel options for periodontal therapy.
Increasing obstructive sleep apnea risk is associated with albuminuria in Korean adults: cross-sectional analysis
Authors: Chang Suk Won , Kang Ju Wan
Published: March 2024

Several studies have shown an association between albuminuria and obstructive sleep apnea (OSA). However, studies on the relationship between the STOP-BANG questionnaire that can screen for OSA and microalbuminuria are still insufficient. Therefore, this study attempted to clarify the relationship between microalbuminuria and OSA risk using the STOP-BANG questionnaire in Korean adults. A total of 7478 participants (3289 men and 4189 women) aged over 40 were enrolled in the Korean National Health and Nutrition Examination Survey from 2019 to 2020. STOP-BANG questionnaire to screen OSA was obtained from subjects. The urinary albumin/creatinine ratio (ACR) and proteinuria were measured via a single dipstick to evaluate renal function. The high OSA risk group had a higher mean ACR value than the low OSA risk group (36.8 ± 172.2 vs 17.7 ± 82.5; P < 0.001). The proportion of subjects with values of 30 ≤ ACR < 300 mg/g (11.9% vs 6.1%; P < 0.001) and ACR > 300 mg/g (2.1% vs 0.7%; P < 0.001) was significantly higher in high OSA risk group. Multivariate logistic regression results confirmed that microalbuminuria (OR 1.279, 95% confidence interval (CI) 1.068–1.532, P = 0.008) was significantly correlated with high OSA risk. In addition, significant correlation with high OSA risk was also found in macroalbuminuria (OR 1.684, 95% CI 1.073–2.530, P = 0.022) and proteinuria (OR 1.355, 95% CI 1.030–1.783, P = 0.030). We confirmed a significant correlation between high OSA risk and albuminuria/proteinuria in Korean adults. Therefore, renal function evaluation is required in high OSA risk patients, and OSA diagnosis through PSG test and treatment is necessary.
RNA-binding proteins potentially regulate the alternative splicing of cell cycle-associated genes in proliferative diabetic retinopathy
Authors: Yang Ning , Zhang Ningzhi , Lu Guojing , Zeng Siyu , Xing Yiqiao , Du Lei
Published: March 2024

RNA-binding proteins (RBPs) contribute to the pathogenesis of proliferative diabetic retinopathy (PDR) by regulating gene expression through alternative splicing events (ASEs). However, the RBPs differentially expressed in PDR and the underlying mechanisms remain unclear. Thus, this study aimed to identify the differentially expressed genes in the neovascular membranes (NVM) and retinas of patients with PDR. The public transcriptome dataset GSE102485 was downloaded from the Gene Expression Omnibus database, and samples of PDR and normal retinas were analyzed. A mouse model of oxygen-induced retinopathy was used to confirm the results. The top 20 RBPs were screened for co-expression with alternative splicing genes (ASGs). A total of 403 RBPs were abnormally expressed in the NVM and retina samples. Functional analysis demonstrated that the ASGs were enriched in cell cycle pathways. Cell cycle-associated ASEs and an RBP–AS regulatory network, including 15 RBPs and their regulated ASGs, were extracted. Splicing factor proline/glutamine rich (SFPQ), microtubule-associated protein 1 B (MAP1B), heat-shock protein 90-alpha (HSP90AA1), microtubule-actin crosslinking factor 1 (MACF1), and CyclinH (CCNH) expression remarkably differed in the mouse model. This study provides novel insights into the RBP–AS interaction network in PDR and for developing screening and treatment options to prevent diabetic retinopathy-related blindness.
Dissociation of H2O2 on water surfaces (ice and water droplets)
Authors: Kumar Amit , Kumar Pradeep
Published: March 2024

In the present work, we have studied the thermal dissociation of H2O2 on water droplets and the surface of ice using Born–Oppenheimer molecular dynamics simulations.
Isomerization of surface functionalized SWCNTs and the critical influence on photoluminescence: static calculations and excited-state dynamics simulations
Authors: Chen Shunwei , Li Yi , Chen Xinxin , Li Lingyun , Lu Qifang , Guo Enyan , Si Conghui , Wei Mingzhi , Han Xiujun
Published: March 2024

The migration of surface chemical groups and static/dynamic excited-state features of as-functionalized SWCNTs were revealed.
The structure-giving role of Rb+ ions for water–ice nanoislands supported on Cu(111)
Authors: Martínez Javier A. , Langguth Inga C. , Olivenza-León David , Morgenstern Karina
Published: March 2024

Solvation of rubidium in contact with the Cu(111) surface is studied by low-temperature scanning tunneling microscopy. Rubidium ions induce changes in the geometry of the water ice island, presenting a structure-building effect.
Cellulose, cellulose derivatives and cellulose composites in sustainable corrosion protection: challenges and opportunities
Authors: Verma Chandrabhan , Singh Vidusha , AlFantazi Akram
Published: March 2024

The use of cellulose-based compounds in coating and aqueous phase corrosion prevention is becoming more popular because they provide excellent protection and satisfy the requirements of green chemistry and sustainable development.
Advances in nanofluidic field-effect transistors: external voltage-controlled solid-state nanochannels for stimulus-responsive ion transport and beyond
Authors: Laucirica G. , Toum-Terrones Y. , Cayón V. M. , Toimil-Molares M. E. , Azzaroni O. , Marmisollé W. A.
Published: March 2024

Nanofluidic field-effect transistors for tailored transport and bio-inspired functionalities: solid-state nanochannels can be smartly tuned by external potentials to induce open/closed states or promote ion selectivity in the same way as biological ion channels.
Emerging polymeric materials for treatment of oral diseases: design strategy towards a unique oral environment
Authors: Jia Bo , Zhang Beibei , Li Jianhua , Qin Jinlong , Huang Yisheng , Huang Mingshu , Ming Yue , Jiang Jingjing , Chen Ran , Xiao Yufen , Du Jianzhong
Published: March 2024

We highlight the recent progress and design strategies of polymeric materials with tailored functions to overcome the highly movable and wet, microbial and inflammatory environment in the oral cavity for treatment of oral diseases.
Opto-combinatorial indexing enables high-content transcriptomics by linking cell images and transcriptomes
Authors: Tsuchida Arata , Kaneko Taikopaul , Nishikawa Kaori , Kawasaki Mayu , Yokokawa Ryuji , Shintaku Hirofumi
Published: March 2024

We introduce a simple integrated analysis method that links cellular phenotypic behaviour with single-cell RNA sequencing by utilizing a combination of optical indices from cells and hydrogel beads.
A cheaper substitute for HRP: ultra-small Cu–Au bimetallic enzyme mimics with infinitesimal steric hindrance to promote catalytic lateral flow immunodetection of clenbuterol
Authors: Hu Huilan , Tian Jiaqi , Shu Rui , Liu Huihui , Wang Shaochi , Yin Xuechi , Wang Jianlong , Zhang Daohong
Published: March 2024

A lateral flow immunoassay (LFIA) is developed for enzyme-catalyzed double-reading determination of clenbuterol (CLE) through the direct electrostatic adsorption of ultra-small copper–gold bimetallic enzyme mimics (USCGs) and monoclonal antibodies.
Recent advances in iodine–DMSO mediated C(sp3)–H functionalization of methyl-azaarenes via Kornblum oxidation
Authors: Acharya Swadhin Swaraj , Patra Sagarika , Barad Liza Mama , Roul Ananya , Parida Bibhuti Bhusan
Published: March 2024

In the iodine–DMSO medium, the methyl group of methyl azaarenes is converted into aldehyde via Kornblum oxidation and trapped in situ by nucleophiles to create azaarene-linked functionalized scaffolds.
In situ fabrication of a robust P–N heterojunction based on Mn–Zn–S–ethylenediamine hybrid nanorods for boosting photocatalytic performance
Authors: Liang Zhi-Yu , Huang Wang-Jun , Zhan Er-Da , Yang Peng , Huang Ren-Kun , Wang Ying , Yan Gui-Yang
Published: March 2024

Multifarious novel photocatalysts based on hybrid nanocomposites have been developed to boost photocatalytic efficiency.
A potential catalyst for α-pinene isomerization: a solid superacid
Authors: Meng Xuan , Chen Yanqi , Wu Wenxing , Liu Naiwang , Shi Li
Published: March 2024

The calcination temperature affects the crystallinity of the catalyst.
Design and application of metal organic frameworks for heavy metals adsorption in water: a review
Authors: Essalmi S. , Lotfi S. , BaQais A. , Saadi M. , Arab M. , Ait Ahsaine H.
Published: March 2024

The growing apprehension surrounding heavy metal pollution in both environmental and industrial contexts has spurred extensive research into adsorption materials aimed at efficient remediation.
Smartphone-based paper strip assay for putrescine and spermidine detection using hybrid organic–inorganic perovskite with Eu3+ complex
Authors: Truong Thi Thuy , Huy Bui The , Huong Le Thi Cam , Truong Hai Bang , Lee Yong-Ill
Published: March 2024

A new method utilizing fluorescent ratiometry is proposed for detecting putrescine and spermidine.
Characterization of nanozyme kinetics for highly sensitive detection
Authors: Panferov Vasily G. , Wang Xiaoqin , Liu Juewen
Published: March 2024

Enzymes (1) and nanozymes (2) have significant differences in the Michaelis–Menten constant. Detection of nanozymes using enzyme substrates results in lower catalytic activity and a significantly higher limit of detection.
Advancements in defect engineering of two-dimensional nanomaterial-based membranes for enhanced gas separation
Authors: Luo Wenjia , Wang Changzheng , Li Xueguo , Liu Jian , Hou Duo , Zhang Xi , Huang Guoxian , Lu Xingwu , Li Yanlong , Zhou Tao
Published: March 2024

Defect engineering of 2D nanomaterials for gas capture in membrane separation processes.
A dynamic covalent approach to [PtnL2n]2n+ cages
Authors: van Hilst Quinn V. C. , Pearcy Aston C. , Preston Dan , Wright L. James , Hartinger Christian G. , Brooks Heather J. L. , Crowley James D.
Published: March 2024

A dynamic covalent approach was exploited to generate a family of homometallic [PtnL2n]2n+ cage (predominantly [Pt2L4]4+ systems) architectures.
Highly active and regioselective hydroaminomethylation of olefins catalyzed by Rh/sulfoxantphos with ZSM-5
Authors: Yuan Xiaoshuang , Zhang Xueqing , Zheng Zhaohui , Sun Shuhui , Jia Xiaofei , Dong Shuxiang
Published: March 2024

Rh/sulfoxantphos-catalyzed hydroaminomethylation has been developed to afford corresponding linear amines with good yields (71–95%) with high regioselectivities (l/b up to 132.4) and excellent catalytic activities (TON of Rh catalyst up to 23 760).
Cyanometallic charge engineering in spin crossover metal–organic frameworks
Authors: Lyu Bang-Heng , Xie Kai-Ping , Cui Wen , Chen Yan-Cong , Chen Guan-Xi , Wu Si-Guo , Tong Ming-Liang
Published: March 2024

By employing the “cyanometallic charge engineering” strategy, cationic/neutral/anionic inverse-Hofmann-type frameworks were constructed with fine-tuning SCO behaviors.
Thermodynamic model for voltammetric responses in conducting redox polymers
Authors: Anishchenko Dmitrii V. , Vereshchagin Anatoliy A. , Kalnin Arseniy Y. , Novoselova Julia V. , Rubicheva Lyubov G. , Potapenkov Vasiliy V. , Lukyanov Daniil A. , Levin Oleg V.
Published: March 2024

Both experimental and modeling research elucidates the voltammetric response dependence of conductive redox polymers on their nature and composition.
Designing a hybrid nanomaterial based on Cr-containing polyoxometalate and graphene oxide as an electrocatalyst for the hydrogen evolution reaction
Authors: Khalaji-Verjani Maryam , Masteri-Farahani Majid
Published: March 2024

A polyoxometalate (POM)-based hybrid nanomaterial is designed via covalent interaction between the Cr(acac)3 complex and [PMo11O39]7−, followed by immobilization on the surface of modified graphene oxide (mGO) for the hydrogen evolution reaction.
α acid fraction from Hop extract exerts an endothelium-derived hyperpolarization vasorelaxant effect through TRPV4 employing the feedforward mechanism of PKCα
Authors: Di Pietro Paola , Salviati Emanuela , Damato Antonio , Prete Valeria , Abate Angela Carmelita , Campiglia Pietro , Vecchione Carmine , Sommella Eduardo , Carrizzo Albino
Published: March 2024

A representative mechanism evoked by the B1 subfraction at vascular levels in resistance arteries. ECs: endothelial cells; SMCs: smooth muscle cells.
Valorisation of the industrial hemp residue from essential oil production by recovery of cannabidiol and chemo-enzymatic conversion to cannabielsoin
Authors: Fiorito Daniele , Tessaro Davide , Sangalli Fabio , Nobbio Celeste , Nebuloni Mario , Vezzini Matteo , Brenna Elisabetta , Parmeggiani Fabio
Published: March 2024

A sustainable method to extract and purify cannabidiol (CBD) from soaked biomass waste from the production of essential oil of industrial hemp is disclosed. A simple and scalable lipase-mediated oxidation of CBD to cannabielsoin is also reported.
From waste to resource: advancements in sustainable lignin modification
Authors: Libretti Celeste , Santos Correa Luis , Meier Michael A. R.
Published: March 2024

An overview on lignin modification and applications possibilities is provided. Importantly, a quantitative comparison of all discussed literature procedures in terms of sustainability is included.
A dual-functional catalyst for selective dicarbamate synthesis via oxidative carbonylation: enhanced methoxylation for suppressing urea polymer formation
Authors: Han Seulgi , You Young-Woo , Jeong Kwanyong , Im Mintaek , Lim Jung-Ae , Kim Soo Min , Lee Jin Hee , Park Ji Hoon
Published: March 2024

The balance between carbonylation and methoxylation rates is pivotal in dicarbamate synthesis via oxidative carbonylation. The superior methoxylation reactivity of Pd/CeO2 prevents urea accumulation, thereby achieving a high yield of dicarbamates.
Correction: Exploring the Mpemba effect: a universal ice pressing enables porous ceramics
Authors: Yang Xiaodan , Shan Yao , Hong Ying , Zhang Zhuomin , Liu Shiyuan , Yan Xiaodong , Gong Xuetian , Zhang Guangzu , Yang Zhengbao
Published: March 2024

Correction for ‘Exploring the Mpemba effect: a universal ice pressing enables porous ceramics’ by Xiaodan Yang et al., Mater. Horiz., 2024, DOI: https://doi.org/10.1039/d3mh01869e.
Design and synthesis of few-layer molybdenum oxide selenide encapsulated in a 3D interconnected nitrogen-doped carbon anode toward high-performance sodium storage
Authors: Qin Yonghong , Abidin Shahriman Zainal , Bin Md Hashim Azhari , Hassan Oskar Hasdinor , Zhao Xiaojun
Published: March 2024

Novel few-layered MoO3–MoSe2 encapsulated in a 3D NC skeleton is constructed to improve the electrochemical performances of SIBs.
Highly crystalline graphite nanofibers as an anode for high-performance potassium-ion batteries
Authors: Sun Kaixuan , Chang Kun , Tan Jinshuo , Sun Chuan-Fu , Liu Qin
Published: March 2024

Graphite nanofibres with high crystallinity and low defect levels for potassium-ion batteries with high initial Coulombic efficiency and long cycling stability are reported in this study.
High-entropy-based nano-materials for sustainable environmental applications
Authors: Das Shubhasikha , Chowdhury Shamik , Tiwary Chandra Sekhar
Published: March 2024

A comprehensive overview of high entropy materials (HEMs), encompassing their sustainable energy and environmental applications.
Accurately simulating electrical double layers structure and formation using all-atom scaled-charge force fields
Authors: He Haoyu , Zhou Jianguo , Yang Lei , Liang Chenglin , Xu Shuaikai , Chen Ming , Mo Tangming
Published: March 2024

This study revealed that the all-atom scaled-charge force field could more accurately simulate charge storage and charging dynamics of the electric double layers compared to other force fields.
Encapsulation of an enzyme-immobilized smart polymer membrane in a metal–organic framework for enhancement of catalytic performance
Authors: Jabeen Rubina , Ali Nasir , Tajwar Muhammad Ali , Liu Yutong , Luo Dong , Li Dan , Qi Li
Published: March 2024

Using liposome as sacrificial template, a strategy was explored for fabrication of enzymes@smart-polymer-membrane@MOF with hollow cavity. A concept was established for improving the catalytic performance of the nano-reactor by varying temperature.
How Toddlers Use Core and Fringe Vocabulary: What's in an Utterance?
Authors: Binger Cathy , Magallanes Priscilla , Miguel Vanessa San , Harrington Nancy , Hahs-Vaughn Debbie
Published: March 2024

Purpose:

Selecting vocabulary for preliterate individuals who use augmentative and alternative communication presents multiple challenges, as the number of symbols provided must be balanced with cognitive, motoric, and other needs. Prioritizing certain types of vocabulary thus becomes a necessity. For example, prioritizing core vocabulary—that is, words that are commonly used across a group of people and contexts—is a common practice that attempts to address some of these issues. However, most core vocabulary research to date has narrowly focused on individual word counts, ignoring other critical aspects of language development such as how vocabulary aligns with typical development and how children use core and fringe vocabulary within their utterances.

Method:

Descriptive and inferential statistics were used to analyze 112 transcripts to describe how typically developing toddlers (aged 2.5 years) use core and fringe vocabulary within their utterances, in reference to a range of commonly used core vocabulary lists.

Results:

Results indicated that the proportion of the toddlers' utterances that consisted of only core, only fringe, or core + fringe vocabulary varied dramatically depending on the size of the core vocabulary list used, with smaller core lists yielding few “core-only” utterances. Furthermore, utterances containing both core and fringe vocabulary were both grammatically and semantically superior to utterances containing only core or only fringe vocabulary, as evidenced by measures such as mean length of utterance and total number of words.

Conclusion:

Thus, relying on word frequency counts is an insufficient basis for selecting vocabulary for aided preliterate communicators.
Forest vegetation of the national park “Koigorodsky” (Komi republic)
Authors: Kanev V.A. , Dubrovskiy Yu.A. , Neshatayev V.Yu. , Shchekalyov R.V. , Danilov D.A.
Published: March 2024

The Koygorodsky National Park was organized by the Resolution of the Government of the Russian Federation dated 07.12.2019. The area is 56.7 thousand hectares. Organizers of the Koygorodsky National Park hypostasized that in the Southern part of Komi Republic where the Park was located spruce and fir forests with nemoral occurred. They also hypostasized also that the forests of Koygorodsky National Park existed for a long period in the conditions of natural dynamics. The purpose of the article is to test these hypotheses. The National Park is located in the eastern part of the East European Plain near the border between meddle and southern subzones of taiga zone. The relief on the most part of the reserve is formed by the edges of the Northern Uvals The first data on the forest vegetation and flora of vascular plants of the Koigorodsky National Park, which has not been studied botanically before, have been obtained. 12 plant associations from five formations of dark coniferous (spruce, fir), light coniferous (pine) and small-leaved (aspen, birch) forests were identified in the classification of forest vegetation of the Koygorodsky National Park. Spruce forests occupy a fairly wide range of sites, were represented by three groups of associations: green-moss, herb-rich and bog moss, and occurred mainly to river valleys and wetlands of the watershed. Aspen forests were represented by green-moss and herbaceous communities and occupied most of the territory of the National Park. Birch, pine and fir forests occupied small areas. The predominance of secondary small-leaved aspen and birch forests in the territory of the National Park refutes the hypothesis that its forests existed for a long time without human influence. At the same time, the hypothesis about the presence of communities with a high proportion of nemoral species in the Koygorodsky National Park was confirmed.
Egg productivity and viability of larvae of promising breeding systems obtained based on foreign silkworm breeds
Authors: Nasirillaev Bakhtiyar , Khudjamatov Safarali
Published: March 2024

This article comparatively analyzes the viability as well as the fertility of silkworms of local breeds and promising breeding systems during the egg and larval periods. Based on the results of the study, average indicators of eggs hatchability, viability and the morbidity degree of larvae of new breeding systems were identified. At the same time, according to the results obtained, new promising systems in the combination Parvoz 1 x foreign and Parvoz 2 x foreign showed an average degree in terms of fertility characteristics. Uzbek-Chinese breeds are important in the rearing of high-quality cocoon raw materials for sericulture in Uzbekistan in the future, and therefore it is advisable further development of scientific research in this direction and bring it to a new level.
Simulation model of crop yields
Authors: Logachev Maxim , Goncharov Dmitrii
Published: March 2024

The article identifies the factors that affect the yield of crops grown in the open ground. Most factors are random in nature, which depending on a variety of conditions can positively or negatively affect crop growth processes. To predict the strategy for growing plants to maximize possible yield requires the development of simulation models that allow repeated virtual experiments, taking into account changes in a variety of parameters. This justifies the need for the study to be conducted. The use of structural analysis methods allowed to establish key objects and processes affecting all stages of plant development, to determine the links between them and the rules of interaction. Based on this, a model of system dynamics was developed, reflecting the processes of plant transition from one state to another, and the parameters affecting the speed of such transition. The developed model can be used as the basis for an expert system that manages agricultural processes.
Modeling processes of digital transformation of organization and waste collection of farming households
Authors: Krasnikova Irina , Kulibaba Irina
Published: March 2024

The article justifies the necessity of using software tools to organize the processes of waste collection, sorting, temporary storage, and disposal for farms and large agro-industrial complexes. To create a specialized software product, formal models of the processes are developed which show all aspects of their implementation. The method of structural analysis is used to identify key objects and corresponding rules of their interaction. The use of Business Process Model and Notation enables the creation of models that depict the interaction of objects and the relationships between tasks, as well as the necessary resources for the developed software product. These models can be used to create a software product and technical documentation supporting the process of its operation, as well as to create job descriptions.
Digital design in the HRM system as a component of the sustainable development strategy
Authors: Fokina Olga , Goncharenko Larisa , Sobol Tatyana , Mottaeva Angela , Mottaeva Asiiat
Published: March 2024

The article is devoted to digital design in the human resource management system and its role in the implementation of the organization's sustainable development strategy. Digitalization of the Russian economy in the field of human resource management is becoming increasingly popular, opening up broad prospects for the economic growth of enterprises and stimulating competition in the market. In turn, the focus on digital transformation of the HRM system is reflected in the strategic management of the enterprise. The purpose of the work is to analyze digital design in the HRM system as a component of the strategic management process in the light of the sustainable development of the organization. A systematic approach was chosen as the leading approach to the research, considering the process of digital transformation as a complex and, at the same time, holistic phenomenon included in the organization’s’s management system. During the research, an analysis of the dynamics of digital transformation implemented in the form of projects in the field of HRM was carried out, trends in the digitalization of personnel management processes were identified, segments of digital design in the HRM system were identified, the main suppliers of digital design solutions were indicated, drivers and problems of digitalization of HRM systems were noted, and the role of digitalization of the HRM system in the sustainable development strategy.