bluuebunny/crossref_metadata_2025 huggingface dataset:
Methotrexate Reduces the Probability of Discontinuation of TNF Inhibitors in Seropositive Patients With Rheumatoid Arthritis. A Real-World Data Analysis
Authors: Hernández-Breijo Borja , Brenis Claudia M. , Plasencia-Rodríguez Chamaida , Martínez-Feito Ana , Novella-Navarro Marta , Pascual-Salcedo Dora , Balsa Alejandro
Published: June 2021

Tumor necrosis factor inhibitors (TNFi) are widely used for the treatment of patients with rheumatoid arthritis (RA), however a considerable percentage of patients discontinued the therapy. The aim of this study is to explore real-world TNFi survival, stratified for seropositivity, and to determine the factors that may influence it. This is a retrospective, observational and longitudinal study, using real-world data of patients, who started their first TNFi therapy between 1999 and 2018 from the RA-PAZ cohort. Patients were considered seropositive if they showed positive serum levels of either RF, ACPA, or both. Treatment survival was analyzed using Kaplan-Meier curves, and Cox proportional hazards models were used to compare the risks of TNFi discontinuation for seronegative and seropositive patients. Of the included 250 patients, 213 (85%) were seropositive. Results showed that TNFi survival did not depend on seropositivity status. However, median survival time was significant longer for seropositive patients who received concomitant MTX compared to patients who did not receive it (median [95% CI]: 3.3 yr. [2.3–4.2] vs. 2.6 yr. [1.7–3.6], respectively; p = 0.008). Furthermore, seropositive patients who received concomitant MTX were 49% less likely to discontinue TNFi therapy than patients who did not receive it (HR: 0.51; 95% CI: 0.35–0.74). In addition, we found that in seropositive patients, the use of prednisone throughout the TNFi treatment was associated with a higher likelihood of therapy discontinuation (OR: 2.30; 95% CI: 1.01–5.23). In conclusion, these data provide evidence to support the use of concomitant MTX in seropositive patients to prolong the effectiveness and the survival of the TNFi therapy. Moreover, the co-administration of prednisone in seropositive patients receiving TNFi was highly associated with TNFi discontinuation.
Selective Nutrient Transport in Bacteria: Multicomponent Transporter Systems Reign Supreme
Authors: Davies James S. , Currie Michael J. , Wright Joshua D. , Newton-Vesty Michael C. , North Rachel A. , Mace Peter D. , Allison Jane R. , Dobson Renwick C.J.
Published: June 2021

Multicomponent transporters are used by bacteria to transport a wide range of nutrients. These systems use a substrate-binding protein to bind the nutrient with high affinity and then deliver it to a membrane-bound transporter for uptake. Nutrient uptake pathways are linked to the colonisation potential and pathogenicity of bacteria in humans and may be candidates for antimicrobial targeting. Here we review current research into bacterial multicomponent transport systems, with an emphasis on the interaction at the membrane, as well as new perspectives on the role of lipids and higher oligomers in these complex systems.
Case Report: A Homozygous Mutation (p.Y62X) of Phospholipase D3 May Lead to a New Leukoencephalopathy Syndrome
Authors: Liu Yi-Hui , Zhang Hai-Feng , Jin Jie-Yuan , Wei Yan-Qiu , Wang Chen-Yu , Fan Liang-Liang , Liu Lv
Published: June 2021

Leukodystrophies are a heterogeneous group of inherited disorders with highly variable clinical manifestations and pathogenetic backgrounds. At present, variants in more than 20 genes have been described and may be responsible for different types of leukodystrophies. Members of the phospholipase D family of enzymes catalyze the hydrolysis of membrane phospholipids. Meanwhile, phospholipase D3 (PLD3) has also been found to exhibit single stranded DNA (ssDNA) acid 5′ exonuclease activity. Variants in phospholipase D3 (PLD3) may increase the risk of Alzheimer's disease and spinocerebellar ataxia, but this hypothesis has not been fully confirmed. In this study, we identified a novel homozygous mutation (NM_012268.3: c.186C>G/ p.Y62X) of PLD3 in a consanguineous family with white matter lesions, hearing and vision loss, and kidney disease by whole exome sequencing. Real-time PCR revealed that the novel mutation may lead to non-sense-mediated messenger RNA (mRNA) decay. This may be the first case report on the homozygous mutation of PLD3 in patients worldwide. Our studies indicated that homozygous mutation of PLD3 may result in a novel leukoencephalopathy syndrome with white matter lesions, hearing and vision loss, and kidney disease.
Young Domestic Pigs (Sus scrofa) Can Perform Pavlovian Eyeblink Conditioning
Authors: Boele Henk-Jan , Joung Sangyun , Fil Joanne E. , Mudd Austin T. , Fleming Stephen A. , Koekkoek Sebastiaan K. E. , Dilger Ryan N.
Published: June 2021

Introduction: Pigs have been an increasingly popular preclinical model in nutritional neuroscience, as their anatomy, physiology, and nutrition requirements are highly comparable to those of humans. Eyeblink conditioning is one of the most well-validated behavioral paradigms in neuroscience to study underlying mechanisms of learning and memory formation in the cerebellum. Eyeblink conditioning has been performed in many species but has never been done on young pigs. Therefore, our aim here was to develop and validate an eyeblink conditioning paradigm in young pigs.

Method: Eighteen intact male pigs were artificially reared from postnatal day 2–30. The eyeblink conditioning setup consisted of a sound-damping box with a hammock that pigs were placed in, which allowed the pig to remain comfortable yet maintain a typical range of head motion. In a delay conditioning paradigm, the conditional stimulus (CS) was a 550 ms blue light-emitting diode (LED), the unconditional stimulus (US) was a 50 ms eye air-puff, the CS-US interval was 500 ms. Starting at postnatal day 14, pigs were habituated for 5 days to the eyeblink conditioning setup, followed by 5 daily sessions of acquisition training (40 paired CS-US trials each day).

Results: The group-averaged amplitude of conditioned eyelid responses gradually increased over the course of the 5 days of training, indicating that pigs learned to make the association between the LED light CS and the air-puff US. A similar increase was found for the conditioned response (CR) probability: the group-averaged CR probability on session 1 was about 12% and reached a CR probability of 55% on day 5. The latency to CR peak time lacked a temporal preference in the first session but clearly showed preference from the moment that animals started to show more CRs in session 2 and onwards whereby the eyelid was maximally closed exactly at the moment that the US would be delivered.

Conclusion: We concluded that 3-week-old pigs have the capability of performing in a cerebellar classical conditioning task, demonstrating for the first time that eyeblink conditioning in young pigs has the potential to be a valuable behavioral tool to measure neurodevelopment.
How Self-Appraisal Is Mediated by the Brain
Authors: Knyazev Gennady G. , Savostyanov Alexander N. , Bocharov Andrey V. , Rudych Pavel D.
Published: June 2021

Self-appraisal is a process that leads to the formation of self-esteem, which contributes to subjective well-being and mental health. Neuroimaging studies link self-esteem with the activity of the medial prefrontal cortex (MPFC), right temporoparietal junction (rTPJ), posterior cingulate cortex (PCC), anterior insula (AIns), and dorsolateral prefrontal cortex. It is not known, however, how the process of self-appraisal itself is mediated by the brain and how different nodes of the self-appraisal network interact with each other. In this study, we used multilevel mediation analysis of functional MRI data recorded during the trait adjective judgment task, treating the emotional valence of adjectives as the predictor, behavioral response as the dependent variable, and brain activity as the mediator. The mediation effect was revealed in the rTPJ. Dynamic causal modeling showed that positive self-descriptions trigger communication within the network, with the rTPJ exerting the strongest excitatory output and MPFC receiving the strongest excitatory input. rAIns receives the strongest inhibitory input and sends exclusively inhibitory connections to other regions pointing out to its role in the processing of negative self-descriptions. Analysis of individual differences showed that in some individuals, self-appraisal is mostly driven by the endorsement of positive self-descriptions and is accompanied by increased activation and communication between rTPJ, MPFC, and PCC. In others, self-appraisal is driven by the rejection of negative self-descriptions and is accompanied by increased activation of rAIns and inhibition of PCC and MPFC. Membership of these groups was predicted by different personality variables. This evidence uncovers different mechanisms of positive self-bias, which may contribute to different facets of self-esteem and are associated with different personality profiles.
Benchmarking Highly Parallel Hardware for Spiking Neural Networks in Robotics
Authors: Steffen Lea , Koch Robin , Ulbrich Stefan , Nitzsche Sven , Roennau Arne , Dillmann Rüdiger
Published: June 2021

Animal brains still outperform even the most performant machines with significantly lower speed. Nonetheless, impressive progress has been made in robotics in the areas of vision, motion- and path planning in the last decades. Brain-inspired Spiking Neural Networks (SNN) and the parallel hardware necessary to exploit their full potential have promising features for robotic application. Besides the most obvious platform for deploying SNN, brain-inspired neuromorphic hardware, Graphical Processing Units (GPU) are well capable of parallel computing as well. Libraries for generating CUDA-optimized code, like GeNN and affordable embedded systems make them an attractive alternative due to their low price and availability. While a few performance tests exist, there has been a lack of benchmarks targeting robotic applications. We compare the performance of a neural Wavefront algorithm as a representative of use cases in robotics on different hardware suitable for running SNN simulations. The SNN used for this benchmark is modeled in the simulator-independent declarative language PyNN, which allows using the same model for different simulator backends. Our emphasis is the comparison between Nest, running on serial CPU, SpiNNaker, as a representative of neuromorphic hardware, and an implementation in GeNN. Beyond that, we also investigate the differences of GeNN deployed to different hardware. A comparison between the different simulators and hardware is performed with regard to total simulation time, average energy consumption per run, and the length of the resulting path. We hope that the insights gained about performance details of parallel hardware solutions contribute to developing more efficient SNN implementations for robotics.
Neuromorphic Devices for Bionic Sensing and Perception
Authors: Zeng Mingyue , He Yongli , Zhang Chenxi , Wan Qing
Published: June 2021

Neuromorphic devices that can emulate the bionic sensory and perceptual functions of neural systems have great applications in personal healthcare monitoring, neuro-prosthetics, and human–machine interfaces. In order to realize bionic sensing and perception, it’s crucial to prepare neuromorphic devices with the function of perceiving environment in real-time. Up to now, lots of efforts have been made in the incorporation of the bio-inspired sensing and neuromorphic engineering in the booming artificial intelligence industry. In this review, we first introduce neuromorphic devices based on diverse materials and mechanisms. Then we summarize the progress made in the emulation of biological sensing and perception systems. Finally, the challenges and opportunities in these fields are also discussed.
Effectiveness of Nutritional Advice for Community-Dwelling Obese Older Adults With Frailty: A Systematic Review and Meta-Analysis
Authors: Yin Yue-Heng , Liu Justina Yat Wa , Fan Tsz Man , Leung Kit Man , Ng Man Wai , Tsang Tsun Yee , Wong Ka Po , Välimäki Maritta
Published: June 2021

Objectives: This systematic review was aimed to examine the effectiveness of nutritional advise interventions compared with usual care, or exercise, or exercise combined with nutritional advice as a means of improving the body weight, body composition, physical function, and psychosocial well-being of frail, obese older adults.

Methods: CINAHL, Cochrane Library, Embase, MEDLINE, PsycINFO, and Scopus databases were searched to identify relevant studies. The quality of the included studies was assessed using Cochrane's risk of bias tool 2. Meta-analysis was performed with respect to body weight and fat mass. Other outcomes were synthesized narratively.

Results: Eight articles (from two studies) with a total of 137 participants were included in the review. The results revealed that nutritional advice was more effective than exercise in reducing body weight and fat mass. The nutritional advice was also beneficial in enhancing physical function and psychosocial well-being. However, it was less effective than exercise or combined interventions in increasing muscle strength and preventing lean mass loss.

Conclusions: Nutritional advice is an essential intervention for reducing body weight and fat mass, for enhancing physical function, and for improving the psychosocial well-being of obese older adults experiencing frailty. The limited number of studies included in this review suggests that there is a need for more well-designed interventional studies in order to confirm these findings.
Establishment of an Ultrasound Malignancy Risk Stratification Model for Thyroid Nodules Larger Than 4 cm
Authors: Xi Xuehua , Wang Ying , Gao Luying , Jiang Yuxin , Liang Zhiyong , Ren Xinyu , Gao Qing , Lai Xingjian , Yang Xiao , Zhu Shenling , Zhao Ruina , Zhang Xiaoyan , Zhang Bo
Published: June 2021

Background

The incidence and mortality of thyroid cancer, including thyroid nodules > 4 cm, have been increasing in recent years. The current evaluation methods are based mostly on studies of patients with thyroid nodules < 4 cm. The aim of the current study was to establish a risk stratification model to predict risk of malignancy in thyroid nodules > 4 cm.

Methods

A total of 279 thyroid nodules > 4 cm in 267 patients were retrospectively analyzed. Nodules were randomly assigned to a training dataset (n = 140) and a validation dataset (n = 139). Multivariable logistic regression analysis was applied to establish a nomogram. The risk stratification of thyroid nodules > 4 cm was established according to the nomogram. The diagnostic performance of the model was evaluated and compared with the American College Radiology Thyroid Imaging Reporting and Data System (ACR TI-RADS), Kwak TI-RADS and 2015 ATA guidelines using the area under the receiver operating characteristic curve (AUC).

Results

The analysis included 279 nodules (267 patients, 50.6 ± 13.2 years): 229 were benign and 50 were malignant. Multivariate regression revealed microcalcification, solid mass, ill-defined border and hypoechogenicity as independent risk factors. Based on the four factors, a risk stratified clinical model was developed for evaluating nodules > 4 cm, which includes three categories: high risk (risk value = 0.8-0.9, with more than 3 factors), intermediate risk (risk value = 0.3-0.7, with 2 factors or microcalcification) and low risk (risk value = 0.1-0.2, with 1 factor except microcalcification). In the validation dataset, the malignancy rate of thyroid nodules > 4 cm that were classified as high risk was 88.9%; as intermediate risk, 35.7%; and as low risk, 6.9%. The new model showed greater AUC than ACR TI-RADS (0.897 vs. 0.855, p = 0.040), but similar sensitivity (61.9% vs. 57.1%, p = 0.480) and specificity (91.5% vs. 93.2%, p = 0.680).

Conclusion

Microcalcification, solid mass, ill-defined border and hypoechogenicity on ultrasound may be signs of malignancy in thyroid nodules > 4 cm. A risk stratification model for nodules > 4 cm may show better diagnostic performance than ACR TI-RADS, which may lead to better preoperative decision-making.
Promoter Methylation of the MGRN1 Gene Predicts Prognosis and Response to Chemotherapy of High-Grade Serous Ovarian Cancer Patients
Authors: Li Xiao-fei , Sun Hai-yan , Hua Tian , Zhang Hai-bo , Tian Yun-jie , Li Yan , Kang Shan
Published: June 2021

Aberrant DNA methylation is considered to play a critical role in the chemoresistance of epithelial ovarian cancer (EOC). In this study, we explored the relationship between hypermethylation of the Mahogunin Ring Finger 1 (MGRN1) gene promoter and primary chemoresistance and clinical outcomes in high-grade serous ovarian cancer (HGSOC) patients. The MALDI-TOF mass spectrometry assays revealed a strong association between hypermethylation of the MGRN1 upstream region and platinum resistance in HGSOC patients. Spearman’s correlation analysis revealed a significantly negative connection between the methylation level of MGRN1 and its expression in HGSOC. In vitro analysis demonstrated that knockdown of MGRN1 reduced the sensitivity of cells to cisplatin and that expression of EGR1 was significantly decreased in SKOV3 cells with low levels of MGRN1 expression. Similarly, EGR1 mRNA expression was lower in platinum-resistant HGSOC patients and was positively correlated with MGRN1 mRNA expression. Kaplan-Meier analyses showed that high methylation of the MGRN1 promoter region and low expression of MGRN1 were associated with worse survival of HGSOC patients. In multivariable models, low MGRN1 expression was an independent factor predicting poor outcome. Furthermore, low expression of EGR1 was also been confirmed to be significantly related to the poor prognosis of HGSOC patients by Kaplan-Meier. The hypermethylation of the MGRN1 promoter region and low expression of MGRN1 were associated with platinum resistance and poor outcomes in HGSOC patients, probably by altering EGR1 expression.
Comprehensive Roles and Future Perspectives of Exosomes in Peritoneal Metastasis of Gastric Cancer
Authors: Chen Xiangliu , Wang Haiyong , Huang Yingying , Chen Yanyan , Chen Chuanzhi , Zhuo Wei , Teng Lisong
Published: June 2021

Gastric cancer (GC) is one of the most prevalent digestive malignancies. A great number of patients at first visit or post curative resections are diagnosed with widespread metastasis within the peritoneal cavity. Overwhelming evidence has demonstrated that exosomes, a variety of biologically functional extracellular vesicles comprising active factors, mediate the progression and metastasis of GC. Although the regulatory mechanisms of exosomes remain fairly elusive, they are responsible for intercellular communication between tumor cells and normal stroma, cancer-related fibroblasts, immune cells within the primary tumor and metastatic niche. In this review, we provide new insight into the molecular signatures of GC-associated exosomes in reprogramming the tumor microenvironment and the subsequent promotion of peritoneal metastasis—including infiltration of the gastric wall, implantation of tumor cells onto the pre-metastatic peritoneum, and remodeling of the pre-metastatic niche. Based on this review, we hope to draw a more general conclusion for the functions of exosomes in the progression and peritoneal metastasis of GC and highlight the future perspective on strategies targeting exosomes in prognostic biomarkers and therapy for peritoneal metastasis.
Mitoprotective Effects of Centella asiatica (L.) Urb.: Anti-Inflammatory and Neuroprotective Opportunities in Neurodegenerative Disease
Authors: Wong Jia Hui , Barron Anna M. , Abdullah Jafri Malin
Published: June 2021

Natural products remain a crucial source of drug discovery for accessible and affordable solutions for healthy aging. Centella asiatica (L.) Urb. (CA) is an important medicinal plant with a wide range of ethnomedicinal uses. Past in vivo and in vitro studies have shown that the plant extract and its key components, such as asiatic acid, asiaticoside, madecassic acid and madecassoside, exhibit a range of anti-inflammatory, neuroprotective, and cognitive benefits mechanistically linked to mitoprotective and antioxidant properties of the plant. Mitochondrial dysfunction and oxidative stress are key drivers of aging and neurodegenerative disease, including Alzheimer’s disease and Parkinson’s disease. Here we appraise the growing body of evidence that the mitoprotective and antioxidative effects of CA may potentially be harnessed for the treatment of brain aging and neurodegenerative disease.
Insulin-Like Peptides and Cross-Talk With Other Factors in the Regulation of Insect Metabolism
Authors: Chowański Szymon , Walkowiak-Nowicka Karolina , Winkiel Magdalena , Marciniak Pawel , Urbański Arkadiusz , Pacholska-Bogalska Joanna
Published: June 2021

The insulin-like peptide (ILP) and insulin-like growth factor (IGF) signalling pathways play a crucial role in the regulation of metabolism, growth and development, fecundity, stress resistance, and lifespan. ILPs are encoded by multigene families that are expressed in nervous and non-nervous organs, including the midgut, salivary glands, and fat body, in a tissue- and stage-specific manner. Thus, more multidirectional and more complex control of insect metabolism can occur. ILPs are not the only factors that regulate metabolism. ILPs interact in many cross-talk interactions of different factors, for example, hormones (peptide and nonpeptide), neurotransmitters and growth factors. These interactions are observed at different levels, and three interactions appear to be the most prominent/significant: (1) coinfluence of ILPs and other factors on the same target cells, (2) influence of ILPs on synthesis/secretion of other factors regulating metabolism, and (3) regulation of activity of cells producing/secreting ILPs by various factors. For example, brain insulin-producing cells co-express sulfakinins (SKs), which are cholecystokinin-like peptides, another key regulator of metabolism, and express receptors for tachykinin-related peptides, the next peptide hormones involved in the control of metabolism. It was also shown that ILPs in Drosophila melanogaster can directly and indirectly regulate AKH. This review presents an overview of the regulatory role of insulin-like peptides in insect metabolism and how these factors interact with other players involved in its regulation.
Genome-Wide Association Mapping of bc-1 and bc-u Reveals Candidate Genes and New Adjustments to the Host-Pathogen Interaction for Resistance to Bean Common Mosaic Necrosis Virus in Common Bean
Authors: Soler-Garzón Alvaro , McClean Phillip E. , Miklas Phillip N.
Published: June 2021

Bean common mosaic necrosis virus (BCMNV) is a major disease in common bean (Phaseolus vulgaris L.). Host plant resistance is the primary disease control. We sought to identify candidate genes to better understand the host-pathogen interaction and develop tools for marker-assisted selection (MAS). A genome-wide association study (GWAS) approach using 182 lines from a race Durango Diversity Panel (DDP) challenged by BCMNV isolates NL-8 [Pathogroup (PG)-III] and NL-3 (PG-VI), and genotyped with 1.26 million single-nucleotide polymorphisms (SNPs), revealed significant peak regions on chromosomes Pv03 and Pv05, which correspond to bc-1 and bc-u resistance gene loci, respectively. Three candidate genes were identified for NL-3 and NL-8 resistance. Side-by-side receptor-like protein kinases (RLKs), Phvul.003G038700 and Phvul.003G038800 were candidate genes for bc-1. These RLKs were orthologous to linked RLKs associated with virus resistance in soybean (Glycine max). A basic Leucine Zipper (bZIP) transcription factor protein is the candidate gene for bc-u. bZIP protein gene Phvul.005G124100 carries a unique non-synonymous mutation at codon 14 in the first exon (Pv05: 36,114,516 bases), resulting in a premature termination codon that causes a nonfunctional protein. SNP markers for bc-1 and bc-u and new markers for I and bc-3 genes were used to genotype the resistance genes underpinning BCMNV phenotypes in the DDP, host group (HG) differentials, and segregating F3 families. Results revealed major adjustments to the current host-pathogen interaction model: (i) there is only one resistance allele bc-1 for the Bc-1 locus, and differential expression of the allele is based on presence vs. absence of bc-u; (ii) bc-1 exhibits dominance and incomplete dominance; (iii) bc-1 alone confers resistance to NL-8; (iv) bc-u was absent from HGs 2, 4, 5, and 7 necessitating a new gene symbol bc-ud to reflect this change; (v) bc-ud alone delays susceptible symptoms, and when combined with bc-1 enhanced resistance to NL-3; and (vi) bc-ud is on Pv05, not Pv03 as previously thought. These candidate genes, markers, and adjustments to the host-pathogen interaction will facilitate breeding for resistance to BCMNV and related Bean common mosaic virus (BCMV) in common bean.
Sleep-Related Breathing Problem Trajectories Across Early Childhood and Academic Achievement-Related Performance at Age Eight
Authors: Harding Rebecca , Schaughency Elizabeth , Haszard Jillian J. , Gill Amelia I. , Luo Rebekah , Lobb Carmen , Dawes Patrick , Galland Barbara
Published: June 2021

Background:Childhood sleep disordered breathing (SDB) has been linked to poorer academic performance; however, research has not investigated the extent improvement in SDB may alter outcomes across key academic skills. This study aimed to investigate if children's early SDB status could predict later academic outcomes, and if an improvement in SDB status across the early childhood years would coincide with better, later performance in key academic skills related to reading, numeracy, and listening comprehension.

Methods:Eighty five case children with an SDB symptom score >25 (maximum 77) were matched to 85 control children (score <12) at recruitment (age 3). SDB severity (symptom history and clinical assessment) was evaluated at ages 3, 4, 6, and 8 years and performance on individually-administered academic skills assessed at age 8 (91% retention from age 3). Case children were categorized into “improved” or “not-improved” groups based on SDB trajectories over the 5 years. Contributions of SDB status and trajectory group to academic performance were determined using regression analysis adjusted for demographic variables.

Results:History of SDB from age 3 predicted significantly poorer performance on some key academic skills (oral reading and listening skills) at age 8. Children whose SDB improved (45%) performed better in oral reading fluency than those whose SDB did not improve, but difficulties with specific tasks involving oral language (listening retell) remained when compared to controls.

Conclusion:Findings support links between early SDB and worse academic outcomes and suggest key academic areas of concern around oral language. Findings highlight the need for child mental health professionals to be aware of children's sleep problems, particularly SDB (past and present), when assessing potential barriers to children's achievement, to assist with appropriate and timely referrals for evaluation of children's sleep difficulties and collaborative evaluation of response to intervention for sleep difficulties.
Message Design Choices Don't Make Much Difference to Persuasiveness and Can't Be Counted On—Not Even When Moderating Conditions Are Specified
Authors: O'Keefe Daniel J. , Hoeken Hans
Published: June 2021

Persuaders face many message design choices: narrative or non-narrative format, gain-framed or loss-framed appeals, one-sided or two-sided messages, and so on. But a review of 1,149 studies of 30 such message variations reveals that, although there are statistically significant differences in persuasiveness between message forms, it doesn't make much difference to persuasiveness which option is chosen (as evidenced by small mean effect sizes, that is, small differences in persuasiveness: median mean rs of about 0.10); moreover, choosing the on-average-more-effective option does not consistently confer a persuasive advantage (as evidenced by 95% prediction intervals that include both positive and negative values). Strikingly, these results obtain even when multiple moderating conditions are specified. Implications for persuasive message research and practice are discussed.
When Depression Breeds Rejection Rather Than Compassion: Disagreeableness, Stigma, and Lack of Empathic Concern Among Support Providers
Authors: Mongrain Myriam , Shoikhedbrod Ariel
Published: June 2021

Past research has shown that the close relationships of depressed individuals are often characterised by rejection rather than compassion. The goal of this research was to broaden interpersonal models of depression by investigating the reports of support providers themselves. Individual differences, including disagreeableness, stigmatic beliefs about depression, and empathic concern were measured. These were examined in relation to reported interpersonal behaviours toward a significant other who was currently depressed. A cross-sectional design was used in an undergraduate (N = 312) and community sample (N = 296). Disagreeable individuals reported less compassionate and more rejecting behaviours toward depressed significant others based on an interpersonal circumplex model of social support. Serial mediation models further indicated that the associations between disagreeableness and rejecting behaviours reported by providers were mediated by stigma and lower empathic concern. The current studies shed light on how the personality, attitudes and emotions of support providers influence the level of compassion expressed toward depressed individuals.
The Prevalence and Correlative Factors of Depression Among Chinese Teachers During the COVID-19 Outbreak
Authors: Zhou Jiaojiao , Yuan Xiaofei , Huang Huanhuan , Li Yaqiong , Yu HongYe , Chen Xu , Luo Jia
Published: June 2021

Background: Epidemiological data on outbreak-associated depression of Chinese teachers are not available. This study aimed to investigate the prevalence and correlates of depression among teachers during the coronavirus disease 2019 (COVID-19) outbreak in mainland China.

Methods: A large cross-sectional online survey was conducted during the COVID-19 outbreak. Depression was assessed using the Patient Health Questionnaire-9 (PHQ-9). The Connor-Davidson Resilience Scale 25 (CD-RISC 25) and Perceived Stress Scale-10 (PSS-10) were used to measure the mental resilience and stress of participants. The correlative factors of depression were analyzed.

Results: In this study, 1,096 teachers were analyzed with a median (range) age of 41 (20–65) years. Of them, 624 (56.9%) suffered from depression (PHQ-9 total score of >4). The multivariate analyses showed that participants with aged ≥41 years (OR = 0.752, 95% CI:0.578–0.979, p = 0.034), participating in epidemic prevention and control (OR = 1.413, 95% CI:1.070–1.867, p = 0.015), thinking prolonged school closure have bad effect (OR = 1.385, 95% CI:1.017–1.885, p = 0.038), sleep duration/day of <6 h (OR = 1.814, 95% CI:1.240–2.655, p < 0.001), physical exercise duration/day of <30 min (OR = 1.619, 95% CI:1.247–2.103, p < 0.001), spending less time with family (OR = 1.729, 95% CI: 1.063–2.655, p = 0.002), being concerned about COVID-19 (OR = 0.609, 95% CI:0.434–0.856, p = 0.004), having poor mental resilience (OR = 6.570, 95% CI:3.533–12.22, p < 0.001) and higher PSS-10 scores (OR = 9.058, 95% CI:3.817–21.50, p < 0.001) were independently associated with depression.

Conclusion: During the COVID-19 outbreak, depression was common among teachers. Age, participating in epidemic prevention and control, opinions toward distant teaching and prolonged school closure, sleep duration/day, physical exercise duration, spending time with family, attitude toward COVID-19, mental resilience and stress represented the independent factors for suffering from depression.
Internet Use, Life Satisfaction, and Subjective Well-Being Among the Elderly: Evidence From 2017 China General Social Survey
Authors: Yang Hua-lei , Wu Yuan-yang , Lin Xue-yu , Xie Lin , Zhang Shuo , Zhang Si-qing , Ti Shi-ming , Zheng Xiao-dong
Published: June 2021

Purpose: The research objectives of this study are to test the scientific propositions of whether Internet use promotes life satisfaction among the elderly, whether there is variability in the effect of Internet use on the well-being of the elderly, and through what channels Internet use affects the elderly's life satisfaction.

Method: Using data from the 2017 Chinese General Social Survey (CGSS), this paper employed linear regression, ordered logit model, and the propensity score matching (PSM) approach to investigate the association of Internet use with life satisfaction among the elderly in China.

Results: The results show that Internet use was significantly and negatively associated with the life satisfaction of the Chinese elderly. Further analysis on group heterogeneity demonstrates that the negative association was more prominent among the participants who were males, at a lower age, had lower income and education, non-agricultural registered, and relatively healthy. Finally, the mediating effect indicates that Internet use may affect life satisfaction among the elderly through the channel of reducing their perceptions of social justice.

Conclusions: In order to avoid the negative effects of using the Internet, the following policy suggestions are put forward: Improve the elderly's attitudes toward Internet use; pay attention to the sense of fairness of the elderly to improve life satisfaction; guide the elderly to reduce the time of using the Internet.
A Comparison of Flower and Grass Strips for Augmentation of Beneficial Arthropods in Apple Orchards
Authors: Dong Zhaoke , Xia Mengjing , Li Cheng , Mu Baofeng , Zhang Zhiyong
Published: June 2021

Sowing plants that provide food resources in orchards is a potential habitat management practice for enhancing biological control. Flowering plants (providing pollen and nectar) and grasses (providing alternative prey) can benefit natural enemies in orchards; however, little is known about their relative importance. We studied the effect of management practices (flower strips, grass strips, and spontaneous grass) on arthropod predators under organic apple management regimes in apple orchards in Beijing, China. Orchards located at two different sites were assessed for 3 years (2017–2019). The cover crops had a significant impact on the abundance and diversity of arthropod predators. The grass treatment consistently supported significantly greater densities of alternative prey resources for predators, and predators were more abundant in the grass than in the other treatments. The Shannon–Wiener diversity was significantly higher for the cover crop treatment than for the control. Community structure was somewhat similar between the grass and control, but it differed between the flower treatment and grass/control. Weak evidence for an increase in mobile predators (ladybirds and lacewings) in the orchard canopy was found. Ladybirds and lacewings were more abundant in the grass treatment than in the other treatments in 2019 only, while the aphid abundance in the grass treatment was lowest. The fact that grass strips promoted higher predator abundance and stronger aphid suppression in comparison to the flower strips suggests that providing alternative prey for predators has great biocontrol service potential. The selection of cover crops and necessary management for conserving natural enemies in orchards are discussed in this paper.
Flow Cytometric Assessment of Ki-67 Expression in Lymphocytes From Physiologic Lymph Nodes, Lymphoma Cell Populations and Remnant Normal Cell Populations From Lymphomatous Lymph Nodes
Authors: Rütgen Barbara C. , Baumgartner Daniel , Fuchs-Baumgartinger Andrea , Rigillo Antonella , Škor Ondřej , Hammer Sabine E. , Saalmüller Armin , Schwendenwein Ilse
Published: June 2021

Recent literature suggests conventional flow cytometric (FCM) immunophenotyping complemented by Ki-67 FCM assessment as a reliable tool to classify canine lymphomas. Ki-67 expression assessed by FCM is rarely reported in canine lymphoma cases and reference data for normal canine lymph nodes are missing. Moreover, nothing is known about the Ki-67 expression within the occasionally observed remnant cell population within the gates of normal lymphocytes in lymphoma cases. Aim of this study was to compare flow cytometric Ki-67 expression of lymphocyte populations from normal canine lymph nodes, lymphoma cells from World-Health-Organisation (WHO) classified lymphoma patient samples and their neighboring normal remnant cell population. Cryopreserved lymphocyte cell suspensions from normal lymph nodes from eight dogs free of lymphoma served as reference material. Fourteen cases diagnosed by cytology, FCM, clonality testing, histopathology including immunohistochemistry consisting of 10 DLBCL, 1 MZL, 1 PTCL and 2 TZL showed a residual small lymphocyte population and were investigated. The Ki-67 expression in normal canine lymphoid tissue was 3.19 ± 2.17%. Mean Ki-67 expression in the malignant cell populations was 41 ± 24.36%. Ki-67 positivity was 12.34 ± 10.68% in the residual physiologic lymphocyte population, which otherwise exhibited a physiologic immunophenotype pattern. This ratio was equivalent (n= 3) or lower (n= 11) than the Ki-67 expression of the malignant cell population within the sample. This is the first report of FCM derived Ki-67 expression combined with immunophenotype patterns in normal canine lymph nodes, compared with lymphoma cell populations and residual normal cell populations of lymphoma cases diagnosed by state of the art technology.
General Methodology for the Identification of Reduced Dynamic Models of Barge-Type Floating Wind Turbines
Authors: Villoslada Daniel , Santos Matilde , Tomás-Rodríguez María
Published: June 2021

Floating offshore wind turbines (FOWT) are designed to overcome some of the limitations of offshore bottom-fixed ones. The development of computational models to simulate the behavior of the structure and the turbine is key to understanding the wind energy system and demonstrating its feasibility. In this work, a general methodology for the identification of reduced dynamic models of barge-type FOWTs is presented. The method is described together with an example of the development of a dynamic model of a 5 MW floating offshore wind turbine. The novelty of the proposed identification methodology lies in the iterative loop relationship between the identification and validation processes. Diversified data sets are used to select the best-fitting identified parameters by cross evaluation of every set among all validating conditions. The data set is generated for different initial FOWT operating conditions. Indeed, an optimal initial condition for platform pitch was found to be far enough from the system at rest to allow the dynamics to be well characterized but not so far that the unmodeled system nonlinearities were so large that they affected significantly the accuracy of the model. The model has been successfully applied to structural control research to reduce fatigue on a barge-type FOWT.
How Task Constraints Influence the Gaze and Motor Behaviours of Elite-Level Gymnasts
Authors: Barreto Joana , Casanova Filipe , Peixoto César , Fawver Bradley , Williams Andrew Mark
Published: June 2021

Perception-action coupling is fundamental to effective motor behaviour in complex sports such as gymnastics. We examined the gaze and motor behaviours of 10 international level gymnasts when performing two skills on the mini-trampoline that matched the performance demands of elite competition. The presence and absence of a vaulting table in each skill served as a task-constraint factor, while we compared super-elite and elite groups. We measured visual search behaviours and kinematic variables during the approach run phase. The presence of a vaulting table influenced gaze behaviour only in the elite gymnasts, who showed significant differences in the time spent fixating on the mini-trampoline, when compared to super-elite gymnasts. Moreover, different approach run characteristics were apparent across the two different gymnastic tasks, irrespective of the level of expertise, and take-off velocity was influenced by the skill being executed across all gymnasts. Task constraints and complexity influence gaze behaviours differed across varying levels of expertise in gymnastics, even within a sample of international level athletes. It appears that the time spent fixating their gazes on the right areas of interest during the approach run is crucial to higher-level performance and therefore higher scores in competition, particularly on the mini-trampoline with vaulting table.
Club Drugs and Psychiatric Sequelae: An Issue of Vulnerability and Previous Psychiatric History
Authors: Martinotti Giovanni , Merino Del Villar Cristina , Garcia Cordoba Andrés , Andrés Tubau Lluís , Castro Sánchez Ivan , Di Carlo Francesco , Chiappini Stefania , Pettorruso Mauro , Schifano Fabrizio , Di Giannantonio Massimo
Published: June 2021

The pursuit of pleasure among clubbers and disco-goers often involves drug use. However, whether substance use may represent a relevant risk factor contributing to the development of psychiatric symptoms and of mental illness remains debated. The purposes of this study were to evaluate the percentage of subjects who developed long-lasting psychiatric symptoms in a sample of subjects reporting use of substances in nightclubs, and to evaluate the role of a previous psychiatric diagnosis in these subjects. Data were collected during three consecutive years in dedicated nursing units inside all the nightclubs of Ibiza, in emergency hospital rooms at the Can Misses Hospital and inside the psychiatric ward. A total of 10,163 subjects required medical assistance inside discos in the medical-nursing units, of which 223 required transfers to hospital emergency rooms. Of these, 110 required subsequent psychiatric hospitalization. Ninety-one (82.7%) of these patients had a positive psychiatric history, which was also found in thirty-one of the 113 subjects (27.4%) not requiring psychiatric hospitalization. Negative psychiatric history was negatively associated with hospitalization (Coefficient = −2.574; p = 0.000) and for subjects with a negative psychiatric history the odds to be hospitalized changed by a factor of 0.076. Gender, age, civil status and nationality were not significant predictors of hospitalization. Overall, the number of subjects who developed major psychiatric disorders appeared to be limited. However, the presence of a psychiatric history here played a crucial role. Club drugs are therefore able to induce psychiatric sequelae requiring hospitalization mainly in subjects who are already vulnerable from a psychopathological point of view.
Taking Students on a Strengths Safari: A Multidimensional Pilot Study of School-Based Wellbeing for Young Neurodiverse Children
Authors: Naples Lauren H. , Tuckwiller Elizabeth D.
Published: June 2021

There is a robust body of psychological research linking youth mental health and academic achievement. However, students in early childhood are rarely represented in this research, and children with disabilities and/or neurological differences are virtually absent. Thus, the present pilot study explored the effects of a structured psychoeducation program designed to enhance school-based wellbeing (SBWB) for young students who are neurodivergent (ND). This study utilized a quasi-experimental design to investigate the effects of the Student Strengths Safari intervention on (1) students’ self-reported covitality and (2) teacher-rated executive functioning to (3) examine data for evidence of a dual-factor model of SBWB. Two classrooms in a suburban, Mid-Atlantic private school were randomly assigned to the waitlist control group (n = 14) (1st grade) and the intervention group (n = 10) (2nd grade), and quantitative data were analyzed at pretest and posttest to determine intervention outcomes. Key findings produced evidence to support (a) a statistically significant interaction effect for improvements in executive functioning relative to the waitlist control group (p = 0.011), and (b) the utility of a new theoretical dual-factor model to advance SBWB for ND students in early elementary education.
Seasonal Succession of Bacterial Communities in Three Eutrophic Freshwater Lakes
Authors: Ji Bin , Liu Cheng , Liang Jiechao , Wang Jian
Published: June 2021

Urban freshwater lakes play an indispensable role in maintaining the urban environment and are suffering great threats of eutrophication. Until now, little has been known about the seasonal bacterial communities of the surface water of adjacent freshwater urban lakes. This study reported the bacterial communities of three adjacent freshwater lakes (i.e., Tangxun Lake, Yezhi Lake and Nan Lake) during the alternation of seasons. Nan Lake had the best water quality among the three lakes as reflected by the bacterial eutrophic index (BEI), bacterial indicator (Luteolibacter) and functional prediction analysis. It was found that Alphaproteobacteria had the lowest abundance in summer and the highest abundance in winter. Bacteroidetes had the lowest abundance in winter, while Planctomycetes had the highest abundance in summer. N/P ratio appeared to have some relationships with eutrophication. Tangxun Lake and Nan Lake with higher average N/P ratios (e.g., N/P = 20) tended to have a higher BEI in summer at a water temperature of 27 °C, while Yezhi Lake with a relatively lower average N/P ratio (e.g., N/P = 14) tended to have a higher BEI in spring and autumn at a water temperature of 9–20 °C. BEI and water temperature were identified as the key parameters in determining the bacterial communities of lake water. Phosphorus seemed to have slightly more impact on the bacterial communities than nitrogen. It is expected that this study will help to gain more knowledge on urban lake eutrophication.
Forest Therapy Alone or with a Guide: Is There a Difference between Self-Guided Forest Therapy and Guided Forest Therapy Programs?
Authors: Kim Jin-Gun , Shin Won-Sop
Published: June 2021

There are generally two types of forest therapy. One is to walk or view the forest alone without a guide, and the other is to be accompanied by a guide. This study aimed to investigate the healing factors and health benefits of self-guided forest therapy and guided forest therapy programs and examine the differences in characteristics between interventions. Thirty-seven undergraduate students participated in a randomized experiment (19 in the self-guided forest therapy and 18 in the guided forest therapy program). Data were collected from 111 self-reported essays after each intervention (three essays per person). Results revealed that the forest healing factors contained four categories in common: auditory element, visual element, tactile element, and olfaction element. Forest therapy’s health benefits included five categories in common: change of mind and body, introspection, change of emotion, cognitive change, and social interaction. Among the typical differences, the self-guided forest therapy group mentioned more keywords related to introspection than the guided forest therapy program group. On the other hand, the guided forest therapy program group mentioned more keywords associated with the change of emotion and social interaction than the self-guided forest therapy. Our findings show that self-guided forest therapy provides an opportunity for self-reflection to focus on and think about one’s inner self. On the other hand, guided forest therapy programs provide positive emotional changes and promoting social bonds through interaction with others. Therefore, because the effects that can be obtained vary depending on the type of forest therapy, participants can utilize forest healing to suit the desired outcomes.