bluuebunny/crossref_metadata_2025 huggingface dataset:
Developing a Scalable Dynamic Norm Menu-Based Intervention to Reduce Meat Consumption
Authors: Sparkman Gregg , Weitz Elizabeth , Robinson Thomas N. , Malhotra Neil , Walton Gregory M.
Published: March 2020

How can we curb the current norm of unsustainable levels of meat consumption? Research on dynamic norms finds that learning that others are starting to eat less meat can inspire people to follow suit. Across four field experiments, we test efforts to scale dynamic-norm messages by incorporating them into restaurant and web-based menus. Studies 1–3 find increases in vegetarian orders when dynamic norms are included in menus (1–2.5 percentage points), although this effect does not always reach statistical significance and varies across populations and analytic models. In Study 4, dynamic norms significantly reduced vegetarian orders. These results raise two critical questions. First, where and with whom should a dynamic norm message reduce meat consumption? Our field data and past theory point to non-high socioeconomic contexts, and contexts where the reference group of people who have changed is meaningful to consumers. Second, how can the treatment be strengthened? Over five online experiments, we find that the visibility of the messages can be greatly improved, and more relatable norm referents can be selected. Although impacts on food orders appear modest, the minimal costs of scaling menu-based dynamic norm messages and the possibility of improving effect sizes make this a promising approach.
The Impact of Socio-Economic Factors on Sediment Load: A Case Study of the Yanhe River Watershed
Authors: Zhong Xue , Jiang Xiaohui , Li Leilei , Xu Jing , Xu Huanyu
Published: March 2020

Under the influence of climate change and human activities, sediment load in rivers has changed significantly, which has a profound impact on the stability of ecosystems and the sustainable development of human beings. Taking the Yanhe River watershed as a case, this paper expounds the dynamic relationship among the Grain for Green Project, social and economic development, population migration, and sediment transport. The variability of sediment load was detected by Pettitt test, the double cumulative curve method, and the regression analysis method, and the effects of climate and human activities on sediment load were quantitatively analyzed. The results showed that 1) from 1956 to 2016, the precipitation of Yanhe River watershed rose slightly in the past 10 years, but the sediment load decreased significantly; 1996 was identified as the catastrophic year of the study period, when the contribution of climate change and human activity to reduced sediment load was 14.1% and 85.9%, respectively. 2) The Grain for Green Project increased the vegetation coverage of the study area from 40.6% to 78.5%. 3) The proportion of agricultural GDP in total GDP decreased from 52.26% to 7.3%, and the proportion of agricultural GDP was positively correlated with sediment transport and cultivated land area (p < 0.01). 4) Population migration resulted in the urbanization rate reaching 40.23%, and the urbanization rate is negatively correlated with sediment load and cultivated land area (p < 0.01), while the cultivated land area is positively correlated with sediment load (p < 0.01). The decrease of cultivated land area makes the sediment load gradually decrease. Therefore, socio-economic factors promote the sustainable development of the river basin.
Short-Term Effect of the Addition of Rice Husk Gasification Slag on the Movement and Transformation of Phosphorus in Different Soil Types
Authors: Bai Yuchao , Zhu Jing , Deng Baoyuan , Shi Haili , Wang Zongkang , Han Dongfang , Duan Jixian , Wang Dehan
Published: March 2020

Rice husk gasification slag (RS) is a type of biochar that is one of the main by-products generated from the production of biomass power with rice husk as the feed. This study aimed to explore the short-term effect of the application of RS on the movement and transformation of fertilizer P in two different soil types through an incubation experiment. The results showed that the RS addition had a significant influence on the diffusive movement of P in soil microsites close to fertilizer placements both in latosolic red soil and fluvo-aquic soil. After 50 d of incubation, most of the WE-P (water-extractable P), AE-P (acid-extractable P), and Olsen-P (available P) were concentrated within 0–5 mm from the fertilization site. WE-P, Olsen-P, and the movement amount of the P in the 0–5 mm soil section were significantly increased at all levels of the RS application in the fertilizer P both in the two soil types. The application of the RS reduced the sorption and precipitation of the fertilizer P in the soil and improved the efficiency of the fertilizer P. The findings presented in this study may be used as references in developing RS applications that reduce losses of fertilizer P and reduce environmental risks.
Technology University Students’ Attitudes towards the English Graduation Benchmark (EGB): A Comparison between English-major and non-English Major Students
Authors: Lai Hsuan-Yau (Tony) , Tu Jih-Fu
Published: March 2020

The English Graduation Benchmark (EGB) is a common criterion adopted by many universities in Taiwan, including both comprehensive and technology universities to monitor their graduates’ English ability. However, this common criterion has caused some debates in recent years about its appropriateness and fairness to students from less advantageous backgrounds. Some universities have decided to stop this policy in practice. This study aims to investigate technology university students’ perspectives and attitudes towards the EGB, as well as to compare and contrast the opinions of students from English and non-English departments. A quantitative method was adopted for this study. The questionnaire included: (1) Participants’ background information, (2) their experience of taking the proficiency tests, and (3) their thoughts on the EGB policy. In total, 360 students participated in this study. The results showed that most technology university students agreed with the establishment of the EGB. Students from English and non-English departments had positive attitudes because the benchmark motivated them to learn English in some way. Last but not least, technology university students considered the EGB to be useful for their future career development. This paper considers the possible implications of these findings for universities and the government, and suggests how they might re-conceptualize their policies to make them more sustainable.
An Introduction to Neighborhood Sustainability Assessment Tool (NSAT) Study for China from Comprehensive Analysis of Eight Asian Tools
Authors: Cheshmehzangi Ali , Dawodu Ayotunde , Song Wangyang , Shi Yuzhu , Wang Yuwei
Published: March 2020

In comparison to city-level and building-level sustainability research, neighborhood-level sustainable urban development is less studied. One of the ways of achieving sustainability at this level is the use of the Neighborhood Sustainability Assessment Tool (NSAT), which focuses on the sustainable urban development of districts, communities, and neighborhoods. NSAT is comprised of urban sustainable indicators and associated points ascribed towards achieving specific urban agendas, called headline sustainability indicators (HSIs) and themes. In China, neighborhood-level sustainability agenda has just been recently established in 2017. Hence, there is an immediate need for NSAT development of multiple cities responding to specific regions of different climate zones in China. As an example, this study utilizes the case of Ningbo City, located in east China, for such NSAT development. This paper provides a comprehensive analytical and comparison study of eight Asian NSATs to highlight compatibilities and extract specific indicators for a new NSAT development for China. The results from this comparative and analytical study, developed through a multidimensional approach of sustainable pathway model (SPM) inform a new NSAT development in a new context. This novel contribution is significant in a context where neighborhood sustainability measures are recently developed. This study serves as the starting point for future research of NSATs in China and other countries.
Hybrid Ćirić Type Graphic Υ,Λ-Contraction Mappings with Applications to Electric Circuit and Fractional Differential Equations
Authors: Ameer Eskandar , Aydi Hassen , Arshad Muhammad , De la Sen Manuel
Published: March 2020

In this paper, we initiate the notion of Ćirić type rational graphic Υ , Λ -contraction pair mappings and provide some new related common fixed point results on partial b-metric spaces endowed with a directed graph G. We also give examples to illustrate our main results. Moreover, we present some applications on electric circuit equations and fractional differential equations.
Enantiomers of Carbohydrates and Their Role in Ecosystem Interactions: A Review
Authors: Lojkova Lea , Vranová Valerie , Formánek Pavel , Drápelová Ida , Brtnicky Martin , Datta Rahul
Published: March 2020

D- and most L-enantiomers of carbohydrates and carbohydrate-containing compounds occur naturally in plants and other organisms. These enantiomers play many important roles in plants including building up biomass, defense against pathogens, herbivory, abiotic stress, and plant nutrition. Carbohydrate enantiomers are also precursors of many plant compounds that significantly contribute to plant aroma. Microorganisms, insects, and other animals utilize both types of carbohydrate enantiomers, but their biomass and excrements are dominated by D-enantiomers. The aim of this work was to review the current knowledge about carbohydrate enantiomers in ecosystems with respect to both their metabolism in plants and occurrence in soils, and to identify critical knowledge gaps and directions for future research. Knowledge about the significance of D- versus L-enantiomers of carbohydrates in soils is rare. Determining the mechanism of genetic regulation of D- and L-carbohydrate metabolism in plants with respect to pathogen and pest control and ecosystem interactions represent the knowledge gaps and a direction for future research.
A Novel Method of Laser Coating Process on Worn-Out Cutter Rings of Tunnel Boring Machine for Eco-Friendly Reuse
Authors: Agrawal Anil Kumar , Chattopadhyaya Somnath , Murthy V. M. S. R. , Legutko Stanisław , Krolczyk Grzegorz
Published: March 2020

Cutter rings form an integral part of tunnel boring machines (TBM). These cutters are deployed in various hard rock tunneling projects. The life of the cutter rings governs the economics of tunneling significantly. This paper presents a novel methodology to enhance hardness and wear resistance of used worn out disc cutters in TBM for eco-friendly reuse. Disc cutters are mainly made of H13 tool steel. To improve the hardness and wear resistance, a layer of tungsten carbide is coated on the used cutter rings. Considering the long operating hours of TBM, cutter rings get worn out due to severe interaction with the hard rock both in compression and rolling mode. Replacement of the cutter-ring is costly, and quite a time consuming and cumbersome job. Refurbishment is always a better option and laser cladding is a novel technique for enhanced life of cutters. It increases the hardness and wear resistance of the cutters to a considerable extent. Cladding is carried out with the help of a laser beam. In this method, a layer of nanoparticles of tungsten carbide powder is deposited on the worn-out surface of the cutters. For carrying out the investigation, different coating parameters are selected based on the central composite design (CCD). With different capacities of laser, a total of 13 samples were prepared at various scanning speeds varying between 200 to 300 mm/min and a level of laser power varying between 100 to 200 W. The coating is critically inspected by various means such as an optical microscope, FESEM, and EDS. Hardness testing was accomplished by Vicker’s hardness testing machine. Wear testing was carried out with the aid of pin on disc setup. The results shows an asymmetrical behavior between the yield parameters (hardness and wear rate) and process parameters (scanning speed and laser power). The hardness values increased from 16% to 95%. A correlation test was conducted between the hardness and wear rate. The results depict a clear negative correlation between them, indicating the advantage of laser coating for reducing cutter ring wear in TBM.
Numerical Simulation of Drag Reduction on a Square Rod Detached with Two Control Rods at Various Gap Spacing via Lattice Boltzmann Method
Authors: Manzoor Raheela , Khalid Asma , Khan Ilyas , Shams-Ul-Islam  , Baleanu Dumitru , Nisar Kottakkaran Sooppy
Published: March 2020

Numerical simulations are performed to examine the effect of size of control rods (d1) and spacing ratio (g) on flow around a square rod with upstream and downstream control rods aligned in-line using the lattice Boltzmann method (LBM). The Reynolds number (Re) is fixed at Re = 160, while the spacing between the main rod and control rods is taken in the range 1 ≤ g ≤ 5 and the size of the control rod is varied between 4 and 20. Seven different types of flow mods are observed in this study at different values of g and d1. Variation in force statistics, like mean drag coefficient (Cdmean), Strouhal number (St), root mean square values of drag (Cdrms) and lift coefficients (Clrms), and percentage reduction in mean drag coefficient is discussed in detail. It was examined that vortex shedding completely suppressed at (g, d1) = (1, 12), (2, 12), and (2, 16) where steady flow mode exists. Moreover, it was found that at large gap spacing, where g = 5, the effect of control rods on the main rod vanishes. Due to this strong vortex shedding produced and as a result, maximum value of Cdmean is found at (g, d1) = (5, 8). The negative values of mean drag force are also observed at some gap spacing and size of control rods are due to the effect of thrust. Furthermore, the maximum percentage reduction in Cdmean is 121%, found at (g, d1) = (2, 20).
Majorization and Coefficient Problems for a General Class of Starlike Functions
Authors: Cho Nak Eun , Oroujy Zahra , Analouei Adegani Ebrahim , Ebadian Ali
Published: March 2020

In the current paper, we study a majorization issue for a general category S * ( ϑ ) of starlike functions, the region of which is often symmetric with respect to the real axis. For various special symmetric functions ϑ , corresponding consequences of the main result are also presented with some relevant connections of the outcomes rendered here with those obtained in recent research. Moreover, coefficient bounds for some majorized functions are estimated.
Behavior of Non-Oscillatory Solutions of Fourth-Order Neutral Differential Equations
Authors: Moaaz Osama , El-Nabulsi Rami Ahmad , Bazighifan Omar
Published: March 2020

In this paper, we deal with the asymptotics and oscillation of the solutions of fourth-order neutral differential equations of the form r t z ‴ t α ′ + q t x α g t = 0 , where z t : = x t + p t x δ t . By using a generalized Riccati transformation, we study asymptotic behavior and derive some new oscillation criteria. Our results extend and improve some well-known results which were published recently in the literature. Symmetry ideas are often invisible in these studies, but they help us decide the right way to study them, and to show us the correct direction for future developments. An example is given to illustrate the importance of our results.
A Low Complexity Channel Estimation and Detection for Massive MIMO Using SC-FDE
Authors: Marques da Silva Mário , Dinis Rui , Guerreiro João
Published: March 2020

5G Communications will support millimeter waves (mm-Wave), alongside the conventional centimeter waves, which will enable much higher throughputs and facilitate the employment of hundreds or thousands of antenna elements, commonly referred to as massive Multiple Input–Multiple Output (MIMO) systems. This article proposes and studies an efficient low complexity receiver that jointly performs channel estimation based on superimposed pilots, and data detection, optimized for massive MIMO (m-MIMO). Superimposed pilots suppress the overheads associated with channel estimation based on conventional pilot symbols, which tends to be more demanding in the case of m-MIMO, leading to a reduction in spectral efficiency. On the other hand, MIMO systems tend to be associated with an increase of complexity and increase of signal processing, with an exponential increase with the number of transmit and receive antennas. A reduction of complexity is obtained with the use of the two proposed algorithms. These algorithms reduce the complexity but present the disadvantage that they generate a certain level of interference. In this article, we consider an iterative receiver that performs the channel estimation using superimposed pilots and data detection, while mitigating the interference associated with the proposed algorithms, leading to a performance very close to that obtained with conventional pilots, but without the corresponding loss in the spectral efficiency.
Toxins of Okadaic Acid-Group Increase Malignant Properties in Cells of Colon Cancer
Authors: Jiménez-Cárcamo Danae , García Carlos , Contreras Héctor R.
Published: March 2020

Diarrhetic shellfish poisoning (DSP) is a syndrome caused by the intake of shellfish contaminated with a group of lipophilic and thermostable toxins, which consists of okadaic acid (OA), dinophysistoxin-1 (DTX-1) and dinophysistoxin-2 (DTX-2). These toxins are potent protein Ser/Thr phosphatase inhibitors, mainly type 1 protein phosphatase (PP1) and type 2A protein phosphatase (PP2A). Different effects have been reported at the cellular, molecular and genetic levels. In this study, changes in cell survival and cell mobility induced by OA, DTX-1 and DTX-2 were determined in epithelial cell lines of the colon and colon cancer. The cell viability results showed that tumoral cell lines were more resistant to toxins than the nontumoral cell line. The results of the functional assays for testing cell migration, evaluation of cell death and the expression of proteins associated with cell adhesion showed a dual effect of toxins since in the nontumoral cell line, a greater induction of cell death, presumably by anoikis, was detected. In the tumoral cell lines, there was an induction of a more aggressive phenotype characterized by increased resistance to toxins, increased migration and increased FAK activation. In tumoral cell lines of colon cancer, OA, DTX-1/DTX-2 induce a more aggressive phenotype.
The Pharmacological Mechanism of Diabetes Mellitus-Associated Overactive Bladder and Its Treatment with Botulinum Toxin A
Authors: Wang Chung-Cheng , Jiang Yung-Hong , Kuo Hann-Chorng
Published: March 2020

Diabetes mellitus (DM) is an independent risk factor for overactive bladder (OAB). The pathophysiology of DM-associated OAB is multifactorial and time-dependent. Diabetic bladder dysfunction is highly associated with diabetic complications, mainly including diabetic neuropathy and atherosclerosis. Chronic systemic inflammation and bladder urothelial inflammation may contribute to the onset of OAB. Intravesical botulinum toxin A (BoNT-A) injection has proved to be a successful treatment for idiopathic and neurogenic OAB. BoNT-A can inhibit the efferent pathways of the bladder as well as the chronic inflammation and hypersensitivity via the afferent pathways. We conducted a review of the published literature in Pubmed using a combination of two keywords, namely “botulinum toxin A” (BoNT-A) and “overactive bladder”, with or without the additional keywords “detrusor overactivity”, “diabetes mellitus”, “inflammation”, and “urodynamic study”. We also reviewed the experience of our research teams, who have published several studies of the association between DM and OAB. Since limited data support the effectiveness and safety of BoNT-A for treating patients with DM-associated OAB, a comprehensive evaluation of diabetic complications and urodynamic study is needed before treatment. In the future, it is imperative to explore the clinical characteristics and inflammatory biomarkers of diabetes as determining predictors of the treatment efficacy.
Parathyroid Hormone: A Uremic Toxin
Authors: Duque Eduardo J. , Elias Rosilene M. , Moysés Rosa M. A.
Published: March 2020

Parathyroid hormone (PTH) has an important role in the maintenance of serum calcium levels. It activates renal 1α-hydroxylase and increases the synthesis of the active form of vitamin D (1,25[OH]2D3). PTH promotes calcium release from the bone and enhances tubular calcium resorption through direct action on these sites. Hallmarks of secondary hyperparathyroidism associated with chronic kidney disease (CKD) include increase in serum fibroblast growth factor 23 (FGF-23), reduction in renal 1,25[OH]2D3 production with a decline in its serum levels, decrease in intestinal calcium absorption, and, at later stages, hyperphosphatemia and high levels of PTH. In this paper, we aim to critically discuss severe CKD-related hyperparathyroidism, in which PTH, through calcium-dependent and -independent mechanisms, leads to harmful effects and manifestations of the uremic syndrome, such as bone loss, skin and soft tissue calcification, cardiomyopathy, immunodeficiency, impairment of erythropoiesis, increase of energy expenditure, and muscle weakness.
Red-Crowned Crane (Grus japonensis) Reproduction Was Improved by Inhibiting Mycotoxins with Montmorillonite in Feed
Authors: Liu Dawei , Gu Chao , Lu Changhu , Wu Qinghua , Kuca Kamil , Wu Wenda
Published: March 2020

The red-crowned crane (Grus japonensis) is a vulnerable bird species. Mycotoxins are toxic substances produced by filamentous fungi and are considered as naturally unavoidable contaminants in animal feed. Our recent survey indicated that feeds designed for captive red-crowned cranes were contaminated with mycotoxins. This study was conducted to investigate the protective effects of the mycotoxin binder montmorillonite on the reproductive behavior, sex hormone levels, and egg quality of red-crowned cranes. Twelve pairs of G. japonensis were divided into four groups, and each group was fed one of the following: a selected diet (with extra low levels of mycotoxins), a regular diet, a selected diet with 0.5% montmorillonite added, or a regular diet with 0.5% montmorillonite added. Consumption of the regular diet decreased courtship and mating behaviors, testosterone concentration, egg weight, and shell thickness. However, feed supplementation with montmorillonite increased the courtship, mating behaviors and testosterone concentration during the pre-breeding period, as well as egg weight and shell thickness. These findings suggest that the addition of dietary montmorillonite is effective for controlling mycotoxins in the feed, resulting in improvements in reproductive behaviors, testosterone concentrations, and some egg quality parameters of the red-crowned crane.
Salt Shock Responses of Microcystis Revealed through Physiological, Transcript, and Metabolomic Analyses
Authors: Georges des Aulnois Maxime , Réveillon Damien , Robert Elise , Caruana Amandine , Briand Enora , Guljamow Arthur , Dittmann Elke , Amzil Zouher , Bormans Myriam
Published: March 2020

The transfer of Microcystis aeruginosa from freshwater to estuaries has been described worldwide and salinity is reported as the main factor controlling the expansion of M. aeruginosa to coastal environments. Analyzing the expression levels of targeted genes and employing both targeted and non-targeted metabolomic approaches, this study investigated the effect of a sudden salt increase on the physiological and metabolic responses of two toxic M. aeruginosa strains separately isolated from fresh and brackish waters, respectively, PCC 7820 and 7806. Supported by differences in gene expressions and metabolic profiles, salt tolerance was found to be strain specific. An increase in salinity decreased the growth of M. aeruginosa with a lesser impact on the brackish strain. The production of intracellular microcystin variants in response to salt stress correlated well to the growth rate for both strains. Furthermore, the release of microcystins into the surrounding medium only occurred at the highest salinity treatment when cell lysis occurred. This study suggests that the physiological responses of M. aeruginosa involve the accumulation of common metabolites but that the intraspecific salt tolerance is based on the accumulation of specific metabolites. While one of these was determined to be sucrose, many others remain to be identified. Taken together, these results provide evidence that M. aeruginosa is relatively salt tolerant in the mesohaline zone and microcystin (MC) release only occurs when the capacity of the cells to deal with salt increase is exceeded.
Development of a Sensitive and Reliable UHPLC-MS/MS Method for the Determination of Multiple Urinary Biomarkers of Mycotoxin Exposure
Authors: Liu Zhezhe , Zhao Xiaoxue , Wu Libiao , Zhou Shuang , Gong Zhiyong , Zhao Yunfeng , Wu Yongning
Published: March 2020

A variety of mycotoxins from different sources frequently contaminate farm products, presenting a potential toxicological concern for animals and human. Mycotoxin exposure has been the focus of attention for governments around the world. To date, biomarkers are used to monitor mycotoxin exposure and promote new understanding of their role in chronic diseases. The goal of this research was to develop and validate a sensitive UHPLC-MS/MS method using isotopically-labeled internal standards suitable for accurate determination of 18 mycotoxin biomarkers, including fumonisins, ochratoxins, Alternaria and emerging Fusarium mycotoxins (fumonisin B1, B2, and B3, hydrolyzed fumonisin B1 and B2, ochratoxin A, B, and alpha, alternariol, alternariol monomethyl ether, altenuene, tentoxin, tenuazonic acid, beauvericin, enniatin A, A1, B, and B1) in human urine. After enzymatic digestion with β-glucuronidase, human urine samples were cleaned up using HLB solid phase extraction cartridges prior to instrument analysis. The multi-mycotoxin and analyte-specific method was validated in-house, providing satisfactory results. The method provided good linearity in the tested concentration range (from LOQ up to 25–500 ng/mL for different analytes), with R2 from 0.997 to 0.999. The limits of quantitation varied from 0.0002 to 0.5 ng/mL for all analytes in urine. The recoveries for spiked samples were between 74.0% and 133%, with intra-day precision of 0.5%–8.7% and inter-day precision of 2.4%–13.4%. This method was applied to 60 urine samples collected from healthy volunteers in Beijing, and 10 biomarkers were found. At least one biomarker was found in all but one of the samples. The high sensitivity and accuracy of this method make it practical for human biomonitoring and mycotoxin exposure assessment.
Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum
Authors: Oliveira Flavio , Diez-Quijada Leticia , Turkina Maria V. , Morais João , Felpeto Aldo Barreiro , Azevedo Joana , Jos Angeles , Camean Ana M. , Vasconcelos Vitor , Martins José Carlos , Campos Alexandre
Published: March 2020

Toxic cyanobacterial blooms are a major contaminant in inland aquatic ecosystems. Furthermore, toxic blooms are carried downstream by rivers and waterways to estuarine and coastal ecosystems. Concerning marine and estuarine animal species, very little is known about how these species are affected by the exposure to freshwater cyanobacteria and cyanotoxins. So far, most of the knowledge has been gathered from freshwater bivalve molluscs. This work aimed to infer the sensitivity of the marine mussel Mytilus galloprovincialis to single as well as mixed toxic cyanobacterial cultures and the underlying molecular responses mediated by toxic cyanobacteria. For this purpose, a mussel exposure experiment was outlined with two toxic cyanobacteria species, Microcystis aeruginosa and Chrysosporum ovalisporum at 1 × 105 cells/mL, resembling a natural cyanobacteria bloom. The estimated amount of toxins produced by M. aeruginosa and C. ovalisporum were respectively 0.023 pg/cell of microcystin-LR (MC-LR) and 7.854 pg/cell of cylindrospermopsin (CYN). After 15 days of exposure to single and mixed cyanobacteria, a depuration phase followed, during which mussels were fed only non-toxic microalga Parachlorella kessleri. The results showed that the marine mussel is able to filter toxic cyanobacteria at a rate equal or higher than the non-toxic microalga P. kessleri. Filtration rates observed after 15 days of feeding toxic microalgae were 1773.04 mL/ind.h (for M. aeruginosa), 2151.83 mL/ind.h (for C. ovalisporum), 1673.29 mL/ind.h (for the mixture of the 2 cyanobacteria) and 2539.25 mL/ind.h (for the non-toxic P. kessleri). Filtering toxic microalgae in combination resulted in the accumulation of 14.17 ng/g dw MC-LR and 92.08 ng/g dw CYN. Other physiological and biochemical endpoints (dry weight, byssus production, total protein and glycogen) measured in this work did not change significantly in the groups exposed to toxic cyanobacteria with regard to control group, suggesting that mussels were not affected with the toxic microalgae. Nevertheless, proteomics revealed changes in metabolism of mussels related to diet, specially evident in those fed on combined cyanobacteria. Changes in metabolic pathways related with protein folding and stabilization, cytoskeleton structure, and gene transcription/translation were observed after exposure and feeding toxic cyanobacteria. These changes occur in vital metabolic processes and may contribute to protect mussels from toxic effects of the toxins MC-LR and CYN.
Coinfections of Novel Polyomavirus, Anelloviruses and a Recombinant Strain of Myxoma Virus-MYXV-Tol Identified in Iberian Hares
Authors: Águeda-Pinto Ana , Kraberger Simona , Lund Michael C. , Gortázar Christian , McFadden Grant , Varsani Arvind , Esteves Pedro J.
Published: March 2020

Viruses are ubiquitous in nature; however, very few have been identified in the Leporid species. In the fall of 2018, an outbreak of myxomatosis in Iberian hares (Lepus granatensis) was reported in Spain and a novel recombinant myxoma virus strain (MYXV-Tol) was identified. To investigate variability within the recombinant region of the MYXV-Tol and identify any potential viral coinfections, samples (ear, eyelid or vaginal) of Iberian hares were collected from Spain and analyzed. The presence of the recombinant region of the MYXV-Tol was confirmed in six out of eleven samples analyzed. Additionally, a polyomavirus (family Polyomaviridae), representing a putative new species, and anelloviruses (family Anelloviridae) belonging to two putative species were identified, some as coinfection with the recombinant MYXV-Tol. The two polyomavirus genomes were identified in two hares and share >99% genome-wide identity. Based on the analysis of their large T-antigen, the new polyomavirus clusters in a distant clade from other mammals sharing <64% amino acid identity. A total of 14 anelloviruses were identified, which share 63–99% genome-wide identity. Overall, our results show a coinfection of different DNA viruses in the studied samples and raise awareness regarding the extensive unsampled diversity of viruses in hares.
Forecasting of Landslides Using Rainfall Severity and Soil Wetness: A Probabilistic Approach for Darjeeling Himalayas
Authors: Abraham Minu Treesa , Satyam Neelima , Pradhan Biswajeet , Alamri Abdullah M.
Published: March 2020

Rainfall induced landslides are creating havoc in hilly areas and have become an important concern for the stakeholders and public. Many approaches have been proposed to derive rainfall thresholds to identify the critical conditions that can initiate landslides. Most of the empirical methods are defined in such a way that it does not depend upon any of the in situ conditions. Soil moisture plays a key role in the initiation of landslides as the pore pressure increase and loss in shear strength of soil result in sliding of soil mass, which in turn are termed as landslides. Hence this study focuses on a Bayesian analysis, to calculate the probability of occurrence of landslides, based on different combinations of severity of rainfall and antecedent soil moisture content. A hydrological model, called Système Hydrologique Européen Transport (SHETRAN) is used for the simulation of soil moisture during the study period and event rainfall-duration (ED) thresholds of various exceedance probabilities were used to characterize the severity of a rainfall event. The approach was used to define two-dimensional Bayesian probabilities for occurrence of landslides in Kalimpong (India), which is a highly landslide susceptible zone in the Darjeeling Himalayas. The study proves the applicability of SHETRAN model for simulating moisture conditions for the study area and delivers an effective approach to enhance the prediction capability of empirical thresholds defined for the region.
Model of Nutrient and Pesticide Outflow with Surface Water to Puck Bay (Southern Baltic Sea)
Authors: Kalinowska Dominika , Wielgat Paweł , Kolerski Tomasz , Zima Piotr
Published: March 2020

Coastal basins are particularly exposed to the adverse impact of anthropogenic stress. In many places, despite only the seasonal increase in the number of residents, progressive urbanization and associated changes in the catchment characteristics are noticeable. Puck Bay is part of the Gulf of Gdansk and belongs to the Baltic Sea. Although the area of Puck Bay is covered by the Natura 2000 Network, this has not saved it from eutrophication problems. As part of the work on a complex coastal basin analysis (WaterPUCK project), the Soil and Water Assessment Tool (SWAT) model was used to determine the agricultural impact on water quality in rivers with a flow into Puck Bay: Reda, Gizdepka, Płutnica, and Błądzikowski Stream. The results include the loads of nutrients and pesticides that flow out from the agricultural areas of Puck community into Puck Bay. In this article, special attention has been paid to the impact of precipitation on the quality of water at the outflow of rivers into the Bay of Puck, because it is a decisive element in the amount of nutrients leached along with surface runoff to watercourses and then into the Gulf. The distribution of precipitation thus affects the amount of nutrients absorbed by plants. Modeling the effects of agricultural practices, taking into account long-term meteorological forecasts, is helpful in attempts to reduce the amount of pollutants entering the Baltic Sea.
Understanding the Role of Organic Matter Cycling for the Spatio-Temporal Structure of PCBs in the North Sea
Authors: Daewel Ute , Yakushev Evgeniy V. , Schrum Corinna , Nizzetto Luca , Mikheeva Elena
Published: March 2020

Using the North Sea as a case scenario, a combined three-dimensional hydrodynamic-biogeochemical-pollutant model was applied for simulating the seasonal variability of the distribution of hydrophobic chemical pollutants in a marine water body. The model was designed in a nested framework including a hydrodynamic block (Hamburg Shelf Ocean Model (HAMSOM)), a biogeochemical block (Oxygen Depletion Model (OxyDep)), and a pollutant-partitioning block (PolPar). Pollutants can be (1) transported via advection and turbulent diffusion, (2) get absorbed and released by a dynamic pool of particulate and dissolved organic matter, and (3) get degraded. Our model results indicate that the seasonality of biogeochemical processes, including production, sinking, and decay, favors the development of hot spots with particular high pollutant concentrations in intermediate waters of biologically highly active regions and seasons, and it potentially increases the exposure of feeding fish to these pollutants. In winter, however, thermal convection homogenizes the water column and destroys the vertical stratification of the pollutant. A significant fraction of the previously exported pollutants is then returned to the water surface and becomes available for exchange with the atmosphere, potentially turning the ocean into a secondary source for pollutants. Moreover, we could show that desorption from aging organic material in the upper aphotic zone is expected to retard pollutants transfer and burial into sediments; thus, it is considerably limiting the effectiveness of the biological pump for pollutant exports.
Flow Measurements Using a Sluice Gate; Analysis of Applicability
Authors: Kubrak Elżbieta , Kubrak Janusz , Kiczko Adam , Kubrak Michał
Published: March 2020

This study analyzes the possibilities of using an irrigation sluice gate in submerged conditions to measure water flow rate. Hydraulic experiments on sluice gate discharge capacity were performed on a model made on a 1:2 scale. Measurements were taken for the submerged flow of the sluice gate. Nomograms and relationships for discharge coefficients of the analyzed sluice gate were developed. The possibility of using the existing nomogram for discharge capacity of the submerged sluice gate to determine the discharge capacity of the modeled gate was also investigated. The effect of narrowing of the sluice gate cross-section resulting from different mounting techniques on its capacity was explained. The analyses confirmed the possibility of using the formulas for the submerged sluice gate to estimate the flow through the irrigation sluice gate.
Spatial Variation in Aragonite Saturation State and the Influencing Factors in Jiaozhou Bay, China
Authors: Li Yunxiao , Zhang Longjun , Xue Liang , Fan Wenhua , Liu Fenwu , Yang Hong
Published: March 2020

Both natural processes and human activities affect seawater calcium carbonate saturation state (Ωarag), while the mechanisms are still far from being clearly understood. This study analysed the seawater surface Ωarag during summer and winter in Jiaozhou Bay (JZB), China, based on two cruises observations performed in January and June 2017. The ranges of Ωarag values were 1.55~2.92 in summer and 1.62~2.15 in winter. Regression analyses were conducted to identify the drivers of the change of Ωarag distribution, and then the relative contributions of temperature, mixing processes and biological processes to the spatial differences in Ωarag were evaluated by introducing the difference between total alkalinity (TA) and dissolved inorganic carbon (DIC) as a proxy for Ωarag. The results showed that biological processes were the main factor affecting the spatial differences in Ωarag, with relative contributions of 70% in summer and 50% in winter. The contributions of temperature (25% in summer and 20% in winter) and the mixing processes (5% in summer and 30% in winter) were lower. The increasing urbanization in offshore areas can further worsen acidification, therefore environmental protection in both offshore and onshore is needed.
Precipitation Characteristic Analysis of the Zhoushan Archipelago: From the View of MSWEP and Rainfall Merging
Authors: Xuan Dangwei , Hu Qingfang , Wang Yintang , Yang Hanbo , Li Lingjie , Wang Leizhi
Published: March 2020

Based on the long series of gauge rainfall data from 1979 to 2015, the performance of Multi-Source Weighted-Ensemble Precipitation (MSWEP) precipitation dataset in the Zhoushan Archipelago and its surrounding sea area in Southeast China was evaluated from a variety of perspectives, and then the Cressman scheme was used to merge MSWEP with surface gauge measurements. It was found that at the spatial scale of 0.1° × 0.1°, MSWEP correctly detected most of the daily rainfall events in the study area. The surface precipitation was generally underestimated, with a relative deviation no more than 10%, but there was a fairly high miss reporting on heavy precipitation. The performance of MSWEP is also obviously characterized with seasonal fluctuation. Compared with the gauge records interpolation results, the accuracy statistics of rainfall dataset generated by merging MSWEP with gauge observations is improved to a certain degree. Especially its comprehensive identification ability of the dry and wet state for daily precipitation has been obviously raised. In addition, the merged data has the mixed characteristics of rain gauge observations and MSWEP in spatial structure. This paper has deepened the understanding of the performance of MSWEP in islands and sea areas, and also strengthened the understanding of the marginal effect of merging gauge data with MSWEP, even other global precipitation datasets.
Fractal-Heuristic Method of Water Quality Sensor Locations in Water Supply Network
Authors: Beata Kowalska , Dariusz Kowalski , Ewa Hołota
Published: March 2020

The article presents a new methodology for the selection of the water quality monitoring sensor locations using the water quality model created by means of the EPANET 2.0 software (United States Environmental Protection Agency (USEPA), Durham, NC, USA). The model represents the propagation of free chlorine in a water supply network, in conjunction with the heuristic method, applying the elements of fractal geometry. In the first stage, a subarea is determined, while in the second, a specific node for the location of the measuring point is indicated. The process of determining the location is based on a ranking method, in which the usefulness of individual subareas and measuring nodes is determined by means of a formula taking into account the amount of water intake, the required level of water supply and the effects of a lack of water supply, as well as the calculated concentration of free chlorine. The application of the method requires the construction and calibration of a numerical model of free chlorine decay in the network, as well as the knowledge on the location of the objects of particular importance for the network users. The proposed method will be applied in an existing water supply network of approximately 50,000 recipients.
Climate and Extreme Rainfall Events in the Mono River Basin (West Africa): Investigating Future Changes with Regional Climate Models
Authors: Amoussou Ernest , Awoye Hervé , Totin Vodounon Henri S. , Obahoundje Salomon , Camberlin Pierre , Diedhiou Arona , Kouadio Kouakou , Mahé Gil , Houndénou Constant , Boko Michel
Published: March 2020

This study characterizes the future changes in extreme rainfall and air temperature in the Mono river basin where the main economic activity is weather dependent and local populations are highly vulnerable to natural hazards, including flood inundations. Daily precipitation and temperature from observational datasets and Regional Climate Models (RCMs) output from REMO, RegCM, HadRM3, and RCA were used to analyze climatic variations in space and time, and fit a GEV model to investigate the extreme rainfalls and their return periods. The results indicate that the realism of the simulated climate in this domain is mainly controlled by the choice of the RCMs. These RCMs projected a 1 to 1.5 °C temperature increase by 2050 while the projected trends for cumulated precipitation are null or very moderate and diverge among models. Contrasting results were obtained for the intense rainfall events, with RegCM and HadRM3 pointing to a significant increase in the intensity of extreme rainfall events. The GEV model is well suited for the prediction of heavy rainfall events although there are uncertainties beyond the 90th percentile. The annual maxima of daily precipitation will also increase by 2050 and could be of benefit to the ecosystem services and socioeconomic activities in the Mono river basin but could also be a threat.
Copula-Based Multivariate Frequency Analysis of the 2012–2018 Drought in Northeast Brazil
Authors: Pontes Filho João Dehon , Souza Filho Francisco de Assis , Martins Eduardo Sávio Passos Rodrigues , Studart Ticiana Marinho de Carvalho
Published: March 2020

The 2012–2018 drought was such an extreme event in the drought-prone area of Northeast Brazil that it triggered a discussion about proactive drought management. This paper aims at understanding the causes and consequences of this event and analyzes its frequency. A consecutive sequence of sea surface temperature anomalies in the Pacific and Atlantic Oceans, at both the decadal and interannual scales, led to this severe and persistent drought. Drought duration and severity were analyzed using run theory at the hydrographic region scale as decision-makers understand impact analysis better at this scale. Copula functions were used to properly model drought joint characteristics as they presented different marginal distributions and an asymmetric behavior. The 2012–2018 drought in Ceará State had the highest mean bivariate return period ever recorded, estimated at 240 years. Considering drought duration and severity simultaneously at the level of the hydrographic regions improves risk assessment. This result advances our understanding of exceptional events. In this sense, the present work proposes the use of this analysis as a tool for proactive drought planning.
Multi-Objective Approach for Determining Optimal Sustainable Urban Drainage Systems Combination at City Scale. The Case of San Luis Potosí (México)
Authors: Zubelzu Sergio , Rodríguez-Sinobas Leonor , Sordo-Ward Alvaro , Pérez-Durán Alan , Cisneros-Almazán Rodolfo
Published: March 2020

A method for determining the optimal Sustainable Urban Drainage Systems (SUDs) combination at city scale is presented in this paper. A comprehensive set of SUDs categories comprising infrastructures aimed at either detaining and locally reusing or infiltrating precipitation are considered. A volumetric water balance is proposed for modelling hydrological processes in urban catchments. A multi-criteria approach combining a cost function and aims for both recharging aquifers and limiting runoff contribution to water courses is proposed to find the optimal SUDs combination. The water balance was run with each possible SUDs combination and the optimal set of SUDs was found. The method was applied to the Metropolitan Area of San Luis Potosí (Mexico). The optimal solutions in this case clearly promoted surface runoff detention and reuse over porous pavements and green roofs but they were sensitive to the considered costs. The SUD requirements to potential new urban developments for each catchment to comply with the original hydrological aims were also studied. The method requires customizing the cost function and using representative climatic data.
Lag in Hydrologic Recovery Following Extreme Meteorological Drought Events: Implications for Ecological Water Requirements
Authors: Liu Qiang , Ma Xiaojing , Yan Sirui , Liang Liqiao , Pan Jihua , Zhang Junlong
Published: March 2020

Hydrological regimes, being strongly impacted by climate change, play a vital role in maintaining the integrity of aquatic river habitats. We investigated lag in hydrologic recovery following extreme meteorological drought events, and we also discussed its implications in the assessment of ecological environment flow. We used monthly anomalies of three specific hydrometeorological variables (precipitation, streamflow, and baseflow) to identify drought, while we used the Chapman–Maxwell method (the CM filter) with recession constant calculated from Automatic Baseflow Identification Technique (ABIT) to separate baseflow. Results showed that: (i) Compared to the default recession parameter (α = 0.925), the CM filter with the ABIT estimate (α = 0.984) separated baseflow more accurately. (ii) Hydrological drought, resulting from meteorological drought, reflected the duration and intensity of meteorological drought; namely, longer meteorological drought periods resulted in longer hydrological drought periods. Interestingly, the time lag in streamflow and baseflow indicated that aquatic ecosystem habitat recovery also lagged behind meteorological drought. (iii) Assessing environmental flow by quantifying drought provided greater detail on hydrological regimes compared to abrupt changes, such as the increased hydrological periods and the different environment flows obtained. Taken together, our results indicated that the hydrological response in streamflow and baseflow (e.g., the time lag and the precipitation recovery rate (Pr)) played a vital role in the assessment of environmental flow.