bluuebunny/crossref_metadata_2025 huggingface dataset:
Single-Phase Microgrid Power Quality Enhancement Strategies: A Comprehensive Review
Authors: Alhaiz Hussain A. , Alsafran Ahmed S. , Almarhoon Ali H.
Published: July 2023

Renewable distributed generators (RDGs) have made inroads in recent power systems owing to the environmental effect of traditional generators and their high consumption of electric energy. The widespread use of RDGs has been a recent trend in numerous nations. The integration complexity and the intermittent nature of RDGs can undermine the security and stability of microgrids (µGs). In order to guarantee the effectiveness, dependability, and quality of the electricity delivered, appropriate control methods are necessary. RDGs are being included in single-phase microgrids (1Ø-µGs) to generate energy closer to the user. The creation of low-voltage µGs allows for increased energy efficiency and improved electrical supply dependability. Nevertheless, the combined power pumped by DGs might create power quality (PQ) difficulties, especially during off-grid operations. The three biggest problems with PQ are reactive-power swapping, voltage and frequency (VαF) variations, and current and voltage (IαV) harmonic falsification associated with 1Ø-µGs; these conditions may affect the operation of µGs. The designed and implemented (primary–secondary control systems) in RDGs are the prevalent strategy discussed in the literature for mitigating these PQ difficulties. Furthermore, emerging grid innovations like the electrical spring offer viable alternatives that might reduce some problems through decentralized operation. Although several research studies have addressed PQ concerns in 3Ø-µGs, not all of these solutions are immediately applicable to their 1Ø equivalents. In this paper, the state of the art and a performance comparison of several PQ enhancement strategies of µGs is discussed. Additionally, the primary difficulties and several PQ approach tactics are highlighted. All vital features from high-quality published articles and new dimensions in this field are presented for mitigating PQ difficulties in 1Ø-µGs.
The Relationship between Microbial Community Succession and Flavor Formation during the Natural Fermentation of Hongqu sufu
Authors: Luo Aiguo , Cheng Zilong , Zhao Jia , Hao Jianwei , Shi Shengli , Hu Bianfang
Published: July 2023

To study the diversity of microbial flora in Hongqu sufu and analyze the characteristics of special flavor compounds, this study took self-made Hongqu sufu as the research object. Dynamic changes in sufu during fermentation were studied. High-throughput sequencing (HTS) was used to analyze changes in the diversity of fungal and bacterial communities during fermentation. The results showed that at the phylum level, the dominant fungal phyla were identified, Mucormyces and Ascomycetes. The dominant bacterial phyla were Proteobacteria and Firmicutes. At the genus level, the dominant fungal genera were identified as Actinomucor, Monascus, and Aspergillus. The dominant bacterial genera were Pseudomonas, Aneurimibacillus, Sphingobacterium, and Bacillus. Headspace solid-phase microextraction gas chromatography–mass spectrometry (HS-SPME-GC-MS) combined with technology that can dynamically change flavor compounds was explored to investigate the correlation between microbiota and flavor compounds. In different stages of fermentation, 75 main volatile organic compounds were identified, including seven alcohols, four acids, 16 alkanes, 14 olefins, seven kinds of aldehydes, two kinds of ketones, 10 kinds of esters, one kind of phenol, one kind of sulfur-containing compound, one benzene, and 12 other compounds. The correlation analysis between flora and flavor compounds showed that the fungi genera Alternaria and Pichia were significantly correlated with most flavor compounds. Bacteria genera including Weissella, Hafnia-Obesumbacterium, and Leuconostoc had a strong positive correlation with ethyl oleate.
Interdisciplinary Analysis of Roman Floor Types in the Villa of Diomedes in the Archaeological Park of Pompeii
Authors: De Martino Giuseppina , Di Ludovico Marco , Dessales Hélène , Prota Andrea
Published: July 2023

The present work presents and discusses an analysis of the floor types of the Villa of Diomedes (Pompeii archaeological park in the Campania region, Italy) from an architectural, archaeological, and structural point of view. In particular, the geometrical-structural parameters of different floor types and the rules used by ancient builders to design them are investigated by means of interdisciplinary research. The links between structural assumptions, archival sources, geometric survey, in situ visual inspections, and archaeological information make it possible to define the geometrical-structural parameters of eleven barrel vaults, three wooden floors, and three sloped wooden roofs (buried and collapsed during the Vesuvius eruption). A specific study of the barrel vaults is presented to investigate the relationships between the structural parameters of the main vaults. Furthermore, a comparison between the vaults’ dimensions obtained from surveys and those produced by formulations in the literature between the 15th and 20th centuries concerning masonry vault designs is presented and discussed. These analyses, carried out within the framework of the Villa of Diomedes interdisciplinary project, were very useful for interpreting the fabrication of the villa and making a 3D reconstruction model of how the villa probably looked in the fateful year of 79 A.D.
Mouse Models for HTLV-1 Infection and Adult T Cell Leukemia
Authors: Nakajima Shinsuke , Okuma Kazu
Published: July 2023

Adult T cell leukemia (ATL) is an aggressive hematologic disease caused by human T cell leukemia virus type 1 (HTLV-1) infection. Various animal models of HTLV-1 infection/ATL have been established to elucidate the pathogenesis of ATL and develop appropriate treatments. For analyses employing murine models, transgenic and immunodeficient mice are used because of the low infectivity of HTLV-1 in mice. Each mouse model has different characteristics that must be considered before use for different HTLV-1 research purposes. HTLV-1 Tax and HBZ transgenic mice spontaneously develop tumors, and the roles of both Tax and HBZ in cell transformation and tumor growth have been established. Severely immunodeficient mice were able to be engrafted with ATL cell lines and have been used in preclinical studies of candidate molecules for the treatment of ATL. HTLV-1-infected humanized mice with an established human immune system are a suitable model to characterize cells in the early stages of HTLV-1 infection. This review outlines the characteristics of mouse models of HTLV-1 infection/ATL and describes progress made in elucidating the pathogenesis of ATL and developing related therapies using these mice.
Magneto-Responsive Textiles for Non-Invasive Heating
Authors: Józefczak Arkadiusz , Kaczmarek Katarzyna , Bielas Rafał , Procházková Jitka , Šafařík Ivo
Published: July 2023

Magneto-responsive textiles have emerged lately as an important carrier in various fields, including biomedical engineering. To date, most research has been performed on single magnetic fibers and focused mainly on the physical characterization of magnetic textiles. Herein, from simple woven and non-woven textiles we engineered materials with magnetic properties that can become potential candidates for a smart magnetic platform for heating treatments. Experiments were performed on tissue-mimicking materials to test the textiles’ heating efficiency in the site of interest. When the heat was induced with magneto-responsive textiles, the temperature increase in tissue-mimicking phantoms depended on several factors, such as the type of basic textile material, the concentration of magnetic nanoparticles deposited on the textile’s surface, and the number of layers covering the phantom. The values of temperature elevation, achieved with the use of magnetic textiles, are sufficient for potential application in magnetic hyperthermia therapies and as heating patches or bandages.
Towards the Definition of the Molecular Hallmarks of Idiopathic Membranous Nephropathy in Serum Proteome: A DIA-PASEF Approach
Authors: Previtali Paolo , Pagani Lisa , Risca Giulia , Capitoli Giulia , Bossi Eleonora , Oliveira Glenda , Piga Isabella , Radice Antonella , Trezzi Barbara , Sinico Renato Alberto , Magni Fulvio , Chinello Clizia
Published: July 2023

Idiopathic membranous nephropathy (IMN) is a pathologically defined disorder of the glomerulus, primarily responsible for nephrotic syndromes (NS) in nondiabetic adults. The underlying molecular mechanisms are still not completely clarified. To explore possible molecular and functional signatures, an optimised mass spectrometry (MS) method based on next-generation data-independent acquisition combined with ion-mobility was applied to serum of patients affected by IMN (n = 15) or by other glomerulopathies (PN) (n = 15). The statistical comparison highlighted a panel of 57 de-regulated proteins with a significant increase in lipoprotein-related proteins (APOC1, APOB, APOA1, APOL1 and LCAT) and a substantial quantitative alteration of key serpins (including A4, D1, A7, A6, F2, F1 and 1) possibly associated with IMN or NS and podocyte stress. A critical dysregulation in metabolisms of lipids (e.g., VLDL assembly and clearance) likely to be related to known hyperlipidemia in IMN, along with involvement of non-classical complement pathways and a putative enrolment of ficolin-2 in sustaining the activation of the lectin-mediated complement system have been pinpointed. Moreover, mannose receptor CD206 (MRC1-down in IMN) and biotinidase (BTD-up in IMN) are able alone to accurately distinguish IMN vs. PN. To conclude, our work provides key proteomic insights into the IMN complexity, opening the way to an efficient stratification of MN patients.
The Clinical Significance of CD73 in Cancer
Authors: Bach Niklas , Winzer Riekje , Tolosa Eva , Fiedler Walter , Brauneck Franziska
Published: July 2023

The search for new and effective treatment targets for cancer immunotherapy is an ongoing challenge. Alongside the more established inhibitory immune checkpoints, a novel potential target is CD73. As one of the key enzymes in the purinergic signalling pathway CD73 is responsible for the generation of immune suppressive adenosine. The expression of CD73 is higher in tumours than in the corresponding healthy tissues and associated with a poor prognosis. CD73, mainly by the production of adenosine, is critical in the suppression of an adequate anti-tumour immune response, but also in promoting cancer cell proliferation, tumour growth, angiogenesis, and metastasis. The upregulation of CD73 and generation of adenosine by tumour or tumour-associated immune cells is a common resistance mechanism to many cancer treatments such as chemotherapy, radiotherapy, targeted therapy, and immunotherapy. Therefore, the inhibition of CD73 represents a new and promising approach to increase therapy efficacy. Several CD73 inhibitors have already been developed and successfully demonstrated anti-cancer activity in preclinical studies. Currently, clinical studies evaluate CD73 inhibitors in different therapy combinations and tumour entities. The initial results suggest that inhibiting CD73 could be an effective option to augment anti-cancer immunotherapeutic strategies. This review provides an overview of the rationale behind the CD73 inhibition in different treatment combinations and the role of CD73 as a prognostic marker.
Effect of Non-Modified as Well as Surface-Modified SiO2 Nanoparticles on Red Blood Cells, Biological and Model Membranes
Authors: Solarska-Ściuk Katarzyna , Męczarska Katarzyna , Jencova Vera , Jędrzejczak Patryk , Klapiszewski Łukasz , Jaworska Aleksandra , Hryć Monika , Bonarska-Kujawa Dorota
Published: July 2023

Nanoparticles are extremely promising components that are used in diagnostics and medical therapies. Among them, silica nanoparticles are ultrafine materials that, due to their unique physicochemical properties, have already been used in biomedicine, for instance, in cancer therapy. The aim of this study was to investigate the cytotoxicity of three types of nanoparticles (SiO2, SiO2-SH, and SiO2-COOH) in relation to red blood cells, as well as the impact of silicon dioxide nanoparticles on biological membranes and liposome models of membranes. The results obtained prove that hemolytic toxicity depends on the concentration of nanoparticles and the incubation period. Silica nanoparticles have a marginal impact on the changes in the osmotic resistance of erythrocytes, except for SiO2-COOH, which, similarly to SiO2 and SiO2-SH, changes the shape of erythrocytes from discocytes mainly towards echinocytes. What is more, nanosilica has an impact on the change in fluidity of biological and model membranes. The research gives a new view of the practical possibilities for the use of large-grain nanoparticles in biomedicine.
Developmental Genetic Basis of Hoxd9 Homeobox Domain Deletion in Pampus argenteus Pelvic Fin Deficiency
Authors: Zhang Shun , Zhang Xiaodong , Zhang Cheng , Xu Shanliang , Wang Danli , Guo Chunyang
Published: July 2023

Pampus argenteus is important for commercial fishery catch species and is an emerging target for aquaculture production. Notably, P. argenteus has a bizarre morphology and lacks pelvic fins. However, the reason for the lack of pelvic fins remains unclear, ultimately leading to frequent upside-down floating of P. argenteus during breeding and marked consumption of physical energy. Some lineages, including whales, fugu, snakes, and seahorse, independently lost the pelvic appendages over evolutionary time. Do different taxa employ the same molecular genetic pathways when they independently evolve similar developmental morphologies? Through analysis of the gene responsible for appendage localization, Hoxd9, it was discovered that the Hox domain was absent in the Hoxd9 gene of P. argenteus, and the Hoxd9b gene lacked the Hox9 activation region, a feature not observed in the Hoxd9 gene of other fish species. Interestingly, those distinctive characteristics are not observed in the Hoxd9 gene of other fish species. To determine the association between the Hoxd9 gene characteristics and the pelvic fin deletion in P. argenteus, the full-length cDNA of the Hoxd9a gene was cloned, and morphological observations of the species’ juveniles were performed using stereomicroscopy and scanning electron microscopy. Thereafter, the tissue localization of Hoxd9a in the species was analyzed at the gene and protein levels. Based on the results, deletion of the Hoxd9a structural domain possibly leads to disruptions in the protein translation and the pelvic fin localization in P. argenteus during its early ontogenetic developmental stage, resulting in the absence of pelvic fins.
Genome-Wide Identification of Aqp Family Related to Spermatogenesis in Turbot (Scophthalmus maximus)
Authors: Wang Xueying , Zhao Ning , Wang Tao , Huang Jinwei , Liu Qinghua , Li Jun
Published: July 2023

The development and maturation of sperm entails intricate metabolic processes involving water molecules, amino acids, hormones, and various substances. Among these processes, the role of aquaporins (aqps) in the testis is crucial. Turbot (Scophthalmus maximus) is a significant marine flatfish species in China; however, natural egg laying in females is not feasible under cultured conditions. Consequently, artificial insemination becomes necessary, requiring the retrieval of sperm and eggs through artificial methods. In this study, we combined genomic, transcriptomics, RT-qPCR, computer-assisted sperm analysis (CASA), and immunohistochemistry to investigate the involvement of the aqp family in spermatogenesis in turbot. Through genomic data analysis, we identified 16 aqps genes dispersed across 13 chromosomes, each exhibiting the characteristic major intrinsic protein (MIP) domain associated with AQPs. The results from RNA-seq and RT-qPCR analysis revealed prominent expression of aqp4, 10, and 12 during the proliferative stage, whereas aqp1 showed primary expression during the mature stage. aqp11 displayed high expression levels during both MSII and MSV stages, potentially contributing significantly to the proliferation and maturation of male germ cells. Conversely, aqp8 showed elevated expression levels during the MSIII, MSIII-IV, and MSIV stages, suggesting its direct involvement in spermiogenesis. Immunohistochemical analysis unveiled the predominant localization of AQP1 protein in male germ cells rather than Sertoli cells, specifically concentrated in the head of sperm within cysts. Furthermore, a noteworthy decline in sperm motility was observed when sperm were subjected to treatment with either the AQP1-specific inhibitor (HgCl2) or the AQP1 antibody. However, no direct correlation was found between the expression of Smaqp1 and sperm quality. Overall, these findings provide new insights into the involvement of aqps in teleost spermatogenesis. Moreover, they hold potential for improving techniques related to sperm activation and cryopreservation, offering valuable knowledge for future advancements in this field.
The Role of TRPM4 in Cardiac Electrophysiology and Arrhythmogenesis
Authors: Hu Yaopeng , Cang Jiehui , Hiraishi Keizo , Fujita Takayuki , Inoue Ryuji
Published: July 2023

The transient receptor potential melastatin 4 (TRPM4) channel is a non-selective cation channel that activates in response to increased intracellular Ca2+ levels but does not allow Ca2+ to pass through directly. It plays a crucial role in regulating diverse cellular functions associated with intracellular Ca2+ homeostasis/dynamics. TRPM4 is widely expressed in the heart and is involved in various physiological and pathological processes therein. Specifically, it has a significant impact on the electrical activity of cardiomyocytes by depolarizing the membrane, presumably via Na+ loading. The TRPM4 channel likely contributes to the development of cardiac arrhythmias associated with specific genetic backgrounds and cardiac remodeling. This short review aims to overview what is known so far about the TRPM4 channel in cardiac electrophysiology and arrhythmogenesis, highlighting its potential as a novel therapeutic target to effectively prevent and treat cardiac arrhythmias.
Molecular Dynamics Modeling for the Determination of Elastic Moduli of Polymer–Single-Walled Carbon Nanotube Composites
Authors: Shamsieva Aigul , Evseev Alexander , Piyanzina Irina , Nedopekin Oleg , Tayurskii Dmitrii
Published: July 2023

The use of carbon nanotubes to improve the mechanical properties of polymers is one of the promising directions in materials science. The addition of single-walled carbon nanotubes (SWCNTs) to a polymer results in significant improvements in its mechanical, electrical, optical, and structural properties. However, the addition of SWCNTs does not always improve the polymer properties. Also, when a certain content of SWCNTs is exceeded, the mechanical properties of the nanocomposite become worse. This article reports the results of computer simulations for predicting the mechanical properties of polymer/single-walled carbon nanotube nanocomposites. The efficiency of reinforcing polymer composites is considered depending on the concentration of carbon nanotubes in the polymer matrix, their size, and structure. The elastic moduli of the nanocomposites are predicted using computer simulations for unit cell tension (0.1%). General trends in the mechanical properties of composites with polypropylene (PP), poly(ethyl methacrylate) (PEMA), polystyrene (PS) matrices, and SWCNTs are shown.
L-Carnitine Suppresses Transient Receptor Potential Vanilloid Type 1 Activation in Human Corneal Epithelial Cells
Authors: Lucius Alexander , Chhatwal Sirjan , Valtink Monika , Reinach Peter S. , Li Aruna , Pleyer Uwe , Mergler Stefan
Published: July 2023

Tear film hyperosmolarity induces dry eye syndrome (DES) through transient receptor potential vanilloid type 1 (TRPV1) activation. L-carnitine is a viable therapeutic agent since it protects against this hypertonicity-induced response. Here, we investigated whether L-carnitine inhibits TRPV1 activation by blocking heat- or capsaicin-induced increases in Ca2+ influx or hyperosmotic stress-induced cell volume shrinkage in a human corneal epithelial cell line (HCE-T). Single-cell fluorescence imaging of calcein/AM-loaded cells or fura-2/AM-labeled cells was used to evaluate cell volume changes and intracellular calcium levels, respectively. Planar patch-clamp technique was used to measure whole-cell currents. TRPV1 activation via either capsaicin (20 µmol/L), hyperosmolarity (≈450 mosmol/L) or an increase in ambient bath temperature to 43 °C induced intracellular calcium transients and augmented whole-cell currents, whereas hypertonicity induced cell volume shrinkage. In contrast, either capsazepine (10 µmol/L) or L-carnitine (1–3 mmol/L) reduced all these responses. Taken together, L-carnitine and capsazepine suppress hypertonicity-induced TRPV1 activation by blocking cell volume shrinkage.
Chitosan–Silica Composites for Adsorption Application in the Treatment of Water and Wastewater from Anionic Dyes
Authors: Blachnio Magdalena , Zienkiewicz-Strzalka Malgorzata , Derylo-Marczewska Anna , Nosach Liudmyla V. , Voronin Eugeny F.
Published: July 2023

A series of new types of composites (biopolymer–silica materials) are proposed as selective and effective adsorbents. A new procedure for the synthesis of chitosan–nanosilica composites (ChNS) and chitosan–silica gel composites (ChSG) using geometrical modification of silica and mechanosorption of chitosan is applied. The highest adsorption efficiency was achieved at pH = 2, hence the desirability of modifications aimed at stabilizing chitosan in such conditions. The amount of chitosan in the synthesis grew to 1.8 times the adsorption capacity for the nanosilica-supported materials and 1.6 times for the silica gel-based composites. The adsorption kinetics of anionic dyes (acid red AR88) was faster for ChNS than for ChSG, which results from a silica-type effect. The various structural, textural, and physicochemical aspects of the chitosan–silica adsorbents were analyzed via small-angle X-ray scattering, scanning electron microscopy, low-temperature gas (nitrogen) adsorption, and potentiometric titration, as well as their adsorption effectiveness towards selected dyes. This indicates the synergistic effect of the presence of dye-binding groups of the chitosan component, and the developed interfacial surface of the silica component in composites.
Red Blood Cells and Endothelium Derived Circulating Extracellular Vesicles in Health and Chronic Heart Failure: A Focus on Phosphatidylserine Dynamics in Vesiculation
Authors: Suades Rosa , Vilella-Figuerola Alba , Padró Teresa , Mirabet Sonia , Badimon Lina
Published: July 2023

Circulating extracellular microvesicles (cEVs) are characterised by presenting surface antigens of parental cells. Since their biogenesis involves the translocation of phosphatidylserine (PS) from the inner to the outer leaflet of the plasma membrane, exposed PS has been considered as a recognition hallmark of cEVs. However, not all cEVs externalise PS. In this study, we have phenotypically and quantitatively characterised cEVs by flow cytometry, paying special attention to the proportions of PS in chronic heart failure patients (cHF; n = 119) and a reference non-HF group (n = 21). PS−-cEVs were predominantly found in both groups. Parental markers showed differential pattern depending on the PS exposure. Endothelium-derived and connexin 43-rich cEVs were mainly PS−-cEVs and significantly increased in cHF. On the contrary, platelet-derived cEVs were mostly PS+ and were increased in the non-HF group. We observed similar levels of PS+- and PS−-cEVs in non-HF subjects when analysing immune cell-derived Evs, but there was a subset-specific difference in cHF patients. Indeed, those cEVs carrying CD45+, CD29+, CD11b+, and CD15+ were mainly PS+-cEVs, while those carrying CD14+, CD3+, and CD56+ were mainly PS−-cEVs. In conclusion, endothelial and red blood cells are stressed in cHF patients, as detected by a high shedding of cEVs. Despite PS+-cEVs and PS−-cEVs representing two distinct cEV populations, their release and potential function as both biomarkers and shuttles for cell communication seem unrelated to their PS content.
A Systematic Review of the Effect of Vericiguat on Patients with Heart Failure
Authors: Sahana Urjosee , Wehland Markus , Simonsen Ulf , Schulz Herbert , Grimm Daniela
Published: July 2023

Despite recent advances in heart failure (HF) therapy, the risk of cardiovascular (CV) mortality, morbidity, and HF hospitalization (HFH) are major challenges in HF treatment. We aimed to review the potential of vericiguat as a treatment option for HF. A systematic literature review was performed using the PubMed database and ClinicalTrials.gov. Four randomized controlled trials were identified, which study the safety and efficacy of vericiguat in HF patients. Vericiguat activates soluble guanylate cyclase (sGC) by binding to the beta-subunit, bypassing the requirement for NO-induced activation. The nitric oxide (NO)–sGC–cyclic guanosine monophosphate (cGMP) pathway plays an essential role in cardiovascular (CV) regulation and the protection of healthy cardiac function but is impaired in HF. Vericiguat reduced the risk of CV death and HFH in HF patients with reduced ejection fraction (HFrEF) but showed no therapeutic effect on HF with preserved ejection fraction (HFpEF). The trials demonstrated a favorable safety profile with most common adverse events such as hypotension, syncope, and anemia. Therefore, vericiguat is recommended for patients with HFrEF and a minimum systolic blood pressure of 100 mmHg. Treatment with vericiguat is considered when the individual patient experiences decompensation despite being on guideline-recommended medication, e.g., angiotensin-converting inhibitor/AT1 receptor antagonist, beta-adrenoceptor antagonist, spironolactone, and sodium-glucose transporter 2 inhibitors. Furthermore, larger studies are required to investigate any potential effect of vericiguat in HFpEF patients. Despite the limitations, vericiguat can be recommended for patients with HFrEF, where standard-of-care is insufficient, and the disease worsens.
Self-Standing Pd-Based Nanostructures for Electrocatalytic CO Oxidation: Do Nanocatalyst Shape and Electrolyte pH Matter?
Authors: Salah Belal , Ipadeola Adewale K. , Abdullah Aboubakr M. , Ghanem Alaa , Eid Kamel
Published: July 2023

Tailoring the shape of Pd nanocrystals is one of the main ways to enhance catalytic activity; however, the effect of shapes and electrolyte pH on carbon monoxide oxidation (COOxid) is not highlighted enough. This article presents the controlled fabrication of Pd nanocrystals in different morphologies, including Pd nanosponge via the ice-cooling reduction of the Pd precursor using NaBH4 solution and Pd nanocube via ascorbic acid reduction at 25 °C. Both Pd nanosponge and Pd nanocube are self-standing and have a high surface area, uniform distribution, and clean surface. The electrocatalytic CO oxidation activity and durability of the Pd nanocube were significantly superior to those of Pd nanosponge and commercial Pd/C in only acidic (H2SO4) medium and the best among the three media, due to the multiple adsorption active sites, uniform distribution, and high surface area of the nanocube structure. However, Pd nanosponge had enhanced COOxid activity and stability in both alkaline (KOH) and neutral (NaHCO3) electrolytes than Pd nanocube and Pd/C, attributable to its low Pd-Pd interatomic distance and cleaner surface. The self-standing Pd nanosponge and Pd nanocube were more active than Pd/C in all electrolytes. Mainly, the COOxid current density of Pd nanocube in H2SO4 (5.92 mA/cm2) was nearly 3.6 times that in KOH (1.63 mA/cm2) and 10.3 times that in NaHCO3 (0.578 mA/cm2), owing to the greater charge mobility and better electrolyte–electrode interaction, as evidenced by electrochemical impedance spectroscopy (EIS) analysis. Notably, this study confirmed that acidic electrolytes and Pd nanocube are highly preferred for promoting COOxid and may open new avenues for precluding CO poisoning in alcohol-based fuel cells.
Short Double-Stranded DNA (≤40-bp) Affects Repair Pathway Choice
Authors: Li Zhentian , Wang Ya
Published: July 2023

To repair ionizing radiation (IR)-induced double strand breaks (DSBs), mammalian cells primarily use canonical non-homologous end-joining (cNHEJ), the homologous recombination (HR) pathway, and the alternative non-homologous end-joining (aEJ) as a backup. These pathways function either compensatively or competitively. High linear energy transfer (LET) compared to low-LET IR kills more cells at the same doses by inhibiting only cNHEJ, but not HR or aEJ. The mechanism remains unclear. The activation of each repair pathway requires the binding of different proteins to DNA fragments of varying lengths. We previously observed an increased generation of small DNA fragments (≤40 bp) in cells following high-LET IR compared to low-LET IR, suggesting that short DNA fragments were one of the major factors interfering with cNHEJ. To provide direct evidence, here we compare the efficiencies of cNHEJ, HR, or aEJ in repairing DSBs containing 30- or 60-bp fragments in vitro and in cells. We show that only cNHEJ but not HR or a-EJ was inefficient for repairing DSBs with 30-bp fragments compared to 60-bp ones, which strongly supports our hypothesis. These results not only enhance our understanding of the DSB repair pathway choice but also hold potential benefits for protection against high-LET IR-induced damage or improving high-LET radiotherapy.
bra-miR167a Targets ARF8 and Negatively Regulates Arabidopsis thaliana Immunity against Plasmodiophora brassicae
Authors: Liao Rujiao , Wei Xiaochun , Zhao Yanyan , Xie Zhengqing , Nath Ujjal Kumar , Yang Shuangjuan , Su Henan , Wang Zhiyong , Li Lin , Tian Baoming , Wei Fang , Yuan Yuxiang , Zhang Xiaowei
Published: July 2023

Clubroot is a soil-borne disease caused by Plasmodiophora brassicae, which can seriously affect the growth and production of cruciferous crops, especially Chinese cabbage crops, worldwide. At present, few studies have been conducted on the molecular mechanism of this disease’s resistance response. In this experiment, we analyzed the bioinformation of bra-miR167a, constructed a silencing vector (STTM167a) and an overexpression vector (OE-miR167a), and transformed them to Arabidopsis to confirm the role of miR167a in the clubroot resistance mechanism of Arabidopsis. Afterwards, phenotype analysis and expression level analysis of key genes were conducted on transgenic plants. From the result, we found that the length and number of lateral roots of silence transgenic Arabidopsis STTM167a was higher than that of WT and OE-miR167a. In addition, the STTM167a transgenic Arabidopsis induced up-regulation of disease resistance-related genes (PR1, PR5, MPK3, and MPK6) at 3 days after inoculation. On the other hand, the auxin pathway genes (TIR1, AFB2, and AFB3), which are involved in maintaining the balance of auxin/IAA and auxin response factor (ARF), were down-regulated. These results indicate that bra-miR167a is negative to the development of lateral roots and auxins, but positive to the expression of resistance-related genes. This also means that the STTM167a can improve the resistance of clubroot by promoting lateral root development and the level of auxin, and can induce resistance-related genes by regulating its target genes. We found a positive correlation between miR167a and clubroot disease, which is a new clue for the prevention and treatment of clubroot disease.
New Gene Markers of Exosomal Regulation Are Involved in Porcine Granulosa Cell Adhesion, Migration, and Proliferation
Authors: Kulus Jakub , Kranc Wiesława , Kulus Magdalena , Bukowska Dorota , Piotrowska-Kempisty Hanna , Mozdziak Paul , Kempisty Bartosz , Antosik Paweł
Published: July 2023

Exosomal regulation is intimately involved in key cellular processes, such as migration, proliferation, and adhesion. By participating in the regulation of basic mechanisms, extracellular vesicles are important in intercellular signaling and the functioning of the mammalian reproductive system. The complexity of intercellular interactions in the ovarian follicle is also based on multilevel intercellular signaling, including the mechanisms involving cadherins, integrins, and the extracellular matrix. The processes in the ovary leading to the formation of a fertilization-ready oocyte are extremely complex at the molecular level and depend on the oocyte’s ongoing relationship with granulosa cells. An analysis of gene expression from material obtained from a primary in vitro culture of porcine granulosa cells was employed using microarray technology. Genes with the highest expression (LIPG, HSD3B1, CLIP4, LOX, ANKRD1, FMOD, SHAS2, TAGLN, ITGA8, MXRA5, and NEXN) and the lowest expression levels (DAPL1, HSD17B1, SNX31, FST, NEBL, CXCL10, RGS2, MAL2, IHH, and TRIB2) were selected for further analysis. The gene expression results obtained from the microarrays were validated using quantitative RT-qPCR. Exosomes may play important roles regarding intercellular signaling between granulosa cells. Therefore, exosomes may have significant applications in regenerative medicine, targeted therapy, and assisted reproduction technologies.
Core Fucosylation Mediated by the FucT-8 Enzyme Affects TRAIL-Induced Apoptosis and Sensitivity to Chemotherapy in Human SW480 and SW620 Colorectal Cancer Cells
Authors: López-Cortés Rubén , Correa Pardo Isabel , Muinelo-Romay Laura , Fernández-Briera Almudena , Gil-Martín Emilio
Published: July 2023

Epithelial cells can undergo apoptosis by manipulating the balance between pro-survival and apoptotic signals. In this work, we show that TRAIL-induced apoptosis can be differentially regulated by the expression of α(1,6)fucosyltransferase (FucT-8), the only enzyme in mammals that transfers the α(1,6)fucose residue to the pentasaccharide core of complex N-glycans. Specifically, in the cellular model of colorectal cancer (CRC) progression formed using the human syngeneic lines SW480 and SW620, knockdown of the FucT-8-encoding FUT8 gene significantly enhanced TRAIL-induced apoptosis in SW480 cells. However, FUT8 repression did not affect SW620 cells, which suggests that core fucosylation differentiates TRAIL-sensitive premetastatic SW480 cells from TRAIL-resistant metastatic SW620 cells. In this regard, we provide evidence that phosphorylation of ERK1/2 kinases can dynamically regulate TRAIL-dependent apoptosis and that core fucosylation can control the ERK/MAPK pro-survival pathway in which SW480 and SW620 cells participate. Moreover, the depletion of core fucosylation sensitises primary tumour SW480 cells to the combination of TRAIL and low doses of 5-FU, oxaliplatin, irinotecan, or mitomycin C. In contrast, a combination of TRAIL and oxaliplatin, irinotecan, or bevacizumab reinforces resistance of FUT8-knockdown metastatic SW620 cells to apoptosis. Consequently, FucT-8 could be a plausible target for increasing apoptosis and drug response in early CRC.
The Review of Current and Proposed Methods of Manufacturing Fir Tree Slots of Turbine Aero Engine Discs
Authors: Buk Jarosław , Sułkowicz Paweł , Szeliga Dariusz
Published: July 2023

This review article presents a summary of currently used and proposed methods of manufacturing fir tree slots of discs in turbine engines. The production of aircraft, including aircraft engines during times of overlapping global economic crises related to the COVID-19 pandemic or the war in Eastern Europe requires a quick response to the changing numbers of passengers and cargo. Similarly, the aviation industry must adapt to these conditions, and thus utilize flexible production methods allowing for a quick change in the design or type of a given part. Due to the constant adoption of new materials for the most critical aero engine parts and the necessity of complying with environmental regulations, it is necessary to search for new methods of manufacturing these parts, including fir tree slots. As an alternative to currently used expensive and energy-intensive broaching, many manufacturers try to implement creep feed grinding CFG or contour milling. However, other manufacturing methods, thus far rarely used for crucial machine parts such as WEDM, ECDM or AWJ, are gaining more and more popularity in the aviation industry. This article presents the advantages and shortcomings of these methods in the context of manufacturing fir tree slots.
Production of Ceramics/Metal Oxide Nanofibers via Electrospinning: New Insights into the Photocatalytic and Bactericidal Mechanisms
Authors: Algethami Jari S. , Amna Touseef , S. Alqarni Laila , Alshahrani Aisha A. , Alhamami Mohsen A. M. , Seliem Amal F. , Al-Dhuwayin Badria H. A. , Hassan M. Shamshi
Published: July 2023

Environmental pollution is steadily rising and is having a negative influence on all living things, especially human beings. The advancement of nanoscience in recent decades has provided potential to address this issue. Functional metal oxide nanoparticles/nanofibers have been having a pull-on effect in the biological and environmental domains of nanobiotechnology. Current work, for the first time, is focusing on the electrospinning production of Zr0.5Sn0.5TiO3/SnO2 ceramic nanofibers that may be utilized to battle lethal infections swiftly and inexpensively. By using characterizations like XRD, FT–IR, FESEM, TEM, PL, and UV–Vis–DRS, the composition, structure, morphology, and optical absorption of samples were determined. The minimum inhibitory concentration (MIC) approach was used to investigate the antibacterial activity. Notably, this research indicated that nanofibers exert antibacterial action against both Gram-positive and Gram-negative bacteria with a MIC of 25 µg/mL. Furthermore, negatively charged E. coli was drawn to positively charged metal ions of Zr0.5Sn0.5TiO3/SnO2, which showed a robust inhibitory effect against E. coli. It was interesting to discover that, compared to pure TiO2, Zr0.5Sn0.5TiO3/SnO2 nanofibers revealed increased photocatalytic activity and exceptional cyclability to the photodegradation of Rhodamine B. The composite completely degrades dye in 30 min with 100% efficacy and excellent (97%) reusability. The synergetic effects of Zr0.5Sn0.5TiO3 and SnO2 may be responsible for increased photocatalytic and bactericidal activity.
Continuous SiC Skeleton-Reinforced Reaction-Bonded Boron Carbide Composites with High Flexural Strength
Authors: Xia Qian , Sun Shihao , Ye Jun , Zhang Cuiping , Ru Hongqiang
Published: July 2023

Reaction-bonded boron carbide (RBBC) composites have broad application prospects due to their low cost and net size sintering. The microstructure, reaction mechanism of boron carbide with molten silicon (Si), and mechanical properties have been substantially studied. However, the mechanical properties strengthening mechanism of reaction-bonded boron carbide composites are still pending question. In this study, dense boron carbide ceramics were fabricated by liquid Si infiltration of B4C-C preforms with dispersed carbon black (CB) as the carbon source. Polyethyleneimine (PEI) with a molecular weight of 1800 was used as the dispersant. CB powders uniformly distributed around boron carbide particles and efficiently protected them from reacting with molten Si. The uniformly distributed CB powders in situ reacted with molten Si and formed uniformly distributed SiC grains, thus forming a continuous boron carbide–SiC ceramic skeleton. Meanwhile, the Si content of the composites was reduced. Using PEI-dispersed CB powders as additional carbon source, the composites’ flexural strength, fracture toughness, and Vickers hardness reach up to 470 MPa, 4.6 MPa·m1/2, and 22 GPa, which were increased by 44%, 15%, and 10%, respectively. The mechanisms of mechanical properties strengthening were also discussed.
Investigation of Severe Plastic Deformation Effects on Magnesium RZ5 Alloy Sheets Using a Modified Multi-Pass Equal Channel Angular Pressing (ECAP) Technique
Authors: Sahoo Partha Sarathi , Mahapatra Manas Mohan , Vundavilli Pandu Ranga , Sabat Rama Krushna , Sirohi Sachin , Kumar Sanjeev
Published: July 2023

The present study investigates the effects of multiple passes of equal channel angular pressing (ECAP) on magnesium alloy sheets with the assistance of an Inconel plunger along with a die setup having a channel angle of 120° and corner angle of 10° operating at a temperature of 200 °C followed by the required heat treatment processes. The microstructural analysis of the sheet samples at various stages of the multi-pass hot ECAP has shown evidence of ultrafine grain refinement (UFG) due to the occurrence of severe plastic deformation. X-ray diffraction analysis has also exhibited the presence of phases like MgZn and CeZn3 which is supposedly responsible for the enhancement of the mechanical properties. As a result, the room temperature tensile and compressive strengths have improved by 6.12% and 6.63%, respectively, after the second pass, and 11.56% and 15.64%, respectively, after the fourth pass of ECAP. Additionally, the hardness of the sheets has increased by 6.49% and 16.64% after the second and fourth pass of hot ECAP, respectively, mainly attributed to the drastic decrease in grain size from 164 μm to 12 μm within four ECAP passes, all these with a negligible change in ductility. This success in the thermomechanical processing of Mg-RZ5 alloy sheets using a die channel angle of 120° with a minimal number of passes of hot ECAP under a controlled equivalent strain, further opens doors for incorporating optimizations and/or additional aspects so as to achieve even better grain refinements, and consequently, mechanical strength improvements thereby catering to the industrial needs of aerospace and construction areas.
Dynamic Hydrogels with Viscoelasticity and Tunable Stiffness for the Regulation of Cell Behavior and Fate
Authors: Zhang Yuhang , Wang Zhuofan , Sun Qingqing , Li Qian , Li Shaohui , Li Xiaomeng
Published: July 2023

The extracellular matrix (ECM) of natural cells typically exhibits dynamic mechanical properties (viscoelasticity and dynamic stiffness). The viscoelasticity and dynamic stiffness of the ECM play a crucial role in biological processes, such as tissue growth, development, physiology, and disease. Hydrogels with viscoelasticity and dynamic stiffness have recently been used to investigate the regulation of cell behavior and fate. This article first emphasizes the importance of tissue viscoelasticity and dynamic stiffness and provides an overview of characterization techniques at both macro- and microscale. Then, the viscoelastic hydrogels (crosslinked via ion bonding, hydrogen bonding, hydrophobic interactions, and supramolecular interactions) and dynamic stiffness hydrogels (softening, stiffening, and reversible stiffness) with different crosslinking strategies are summarized, along with the significant impact of viscoelasticity and dynamic stiffness on cell spreading, proliferation, migration, and differentiation in two-dimensional (2D) and three-dimensional (3D) cell cultures. Finally, the emerging trends in the development of dynamic mechanical hydrogels are discussed.
3D Printing of Habitats on Mars: Effects of Low Temperature and Pressure
Authors: Hedayati Reza , Stulova Victoria
Published: July 2023

Due to payload weight limitations and human vulnerability to harsh space conditions, it is preferable that the potential landing location for humans has an already constructed habitat preferably made from in situ materials. Therefore, the prospect of utilizing a readily available Martian material, such as regolith, in an easily programmable manufacturing method, such as 3D printing, is very lucrative. The goal of this research is to explore a mixture containing Martian regolith for the purposes of 3D printing in unfavorable conditions. A binder consisting of water and sodium silicate is used. Martian conditions are less favorable for the curing of such a mixture because of low temperature and pressure on the surface of the planet. In order to evaluate mechanical properties of the mixture, molding and 3D printing were conducted at various curing conditions and the mechanical and physical characteristics were compared. Due to the combination of low reaction speed at low temperature (2 °C) and rapid water evaporation at low pressure (0.1–0.01 bar), curing of the specimens in Martian conditions yielded unsatisfactory results. The reaction medium (water) evaporated before the curing reaction could progress enough to form a proper geopolymer. The specimens cured at high temperatures (60 °C) showed satisfactory results, with flexural strength up to 9 MPa when cured at a temperature of 60 °C and pressure of 1 bar. The specimens manufactured by 3D printing showed ultimate flexural strength that was 20% lower than that of equivalent molded specimens. Exploring potential mixture modifications and performing improved tests using the basis laid in this research can lead to an effective and realistic way of utilizing Martian regolith for unmanned 3D-printing purposes with minimal investment.
Zinc Evaporation from Brass Scraps in the Atmosphere of Inert Gas
Authors: Wilk Magdalena , Matula Tomasz , Blacha Leszek , Smalcerz Albert , Labaj Jerzy
Published: July 2023

A description of the process of metal evaporation from liquid alloys at an atmospheric pressure has a practical value for both the smelting and remelting of their scraps. The quantities of volatile components that are eliminated in these processes depend on many factors of which the type of melting device, the method and conditions of the process performance, the alloy composition and the kind of applied atmosphere are of the greatest importance. In this paper, the results of the research on zinc evaporation from brass scraps containing 10.53 wt% Zn are presented. The experiments were conducted using the thermogravimetric method at 1080 ÷ 1240 °C in a helium atmosphere. In the research, the levels of zinc removal from copper ranged between 82% and 99%. The values of the overall mass transfer coefficient for zinc kZn, determined based on the experimental data, ranged from 4.74 to 8.46 × 10−5 ms−1. The kinetic analysis showed that the rate of the analysed process was determined by mass transfer in the gas phase.
Antibacterial and Biocompatible Polyethylene Composites with Hybrid Clay Nanofillers
Authors: Klecandová Lenka , Nakonieczny Damian S. , Reli Magda , Simha Martynková Gražyna
Published: July 2023

Low-density polyethylene is one of the basic polymers used in medicine for a variety of purposes; so, the relevant improvements in functional properties are discussed here, making it safer to use as devices or implants during surgery or injury. The objective of the laboratory-prepared material was to study the antimicrobial and biocompatible properties of low-density polyethylene composites with 3 wt. % hybrid nanoclay filler. We found that the antimicrobial activity was mainly related to the filler, i.e., the hybrid type, where inorganic clay minerals, vermiculite or montmorillonite, were intercalated with organic chlorhexidine diacetate and subsequently decorated with Ca-deficient hydroxyapatite. After fusion of the hybrid nanofiller with polyethylene, intense exfoliation of the clay layers occurred. This phenomenon was confirmed by the analysis of the X-ray diffraction patterns of the composite, where the original basal peak of the clays decreased or completely disappeared, and the optimal distribution of the filler was observed using the transmission mode of light microscopy. Functional property testing showed that the composites have good antibacterial activity against Staphylococcus aureus, and the biocompatibility prediction demonstrated the formation of Ca- and P-containing particles through an in vitro experiment, thus applicable for medical use.