bluuebunny/crossref_metadata_2025 huggingface dataset:
Compartmentalized spatial profiling of the tumor microenvironment in head and neck squamous cell carcinoma identifies immune checkpoint molecules and tumor necrosis factor receptor superfamily members as biomarkers of response to immunotherapy
Authors: Sadeghirad Habib , Liu Ning , Monkman James , Ma Ning , Cheikh Bassem Ben , Jhaveri Niyati , Tan Chin Wee , Warkiani Majid Ebrahimi , Adams Mark N. , Nguyen Quan , Ladwa Rahul , Braubach Oliver , O’Byrne Ken , Davis Melissa , Hughes Brett G. M. , Kulasinghe Arutha
Published: April 2023

Mucosal head and neck squamous cell carcinoma (HNSCC) are the seventh most common cancer, with approximately 50% of patients living beyond 5 years. Immune checkpoint inhibitors (ICIs) have shown promising results in patients with recurrent or metastatic (R/M) disease, however, only a subset of patients benefit from immunotherapy. Studies have implicated the tumor microenvironment (TME) of HNSCC as a major factor in therapy response, highlighting the need to better understand the TME, particularly by spatially resolved means to determine cellular and molecular components. Here, we employed targeted spatial profiling of proteins on a cohort of pre-treatment tissues from patients with R/M disease to identify novel biomarkers of response within the tumor and stromal margins. By grouping patient outcome categories into response or non-response, based on Response Evaluation Criteria in Solid Tumors (RECIST) we show that immune checkpoint molecules, including PD-L1, B7-H3, and VISTA, were differentially expressed. Patient responders possessed significantly higher tumor expression of PD-L1 and B7-H3, but lower expression of VISTA. Analysis of response subgroups indicated that tumor necrosis factor receptor (TNFR) superfamily members including OX40L, CD27, 4-1BB, CD40, and CD95/Fas, were associated with immunotherapy outcome. CD40 expression was higher in patient-responders than non responders, while CD95/Fas expression was lower in patients with partial response (PR) relative to those with stable disease (SD) and progressive disease (PD). Furthermore, we found that high 4-1BB expression in the tumor compartment, but not in the stroma, was associated with better overall survival (OS) (HR= 0.28, p-adjusted= 0.040). Moreover, high CD40 expression in tumor regions (HR= 0.27, p-adjusted= 0.035), and high CD27 expression in the stroma (HR= 0.2, p-adjusted=0.032) were associated with better survival outcomes. Taken together, this study supports the role of immune checkpoint molecules and implicates the TNFR superfamily as key players in immunotherapy response in our cohort of HNSCC. Validation of these findings in a prospective study is required to determine the robustness of these tissue signatures.
Parameters for the depth of the ocean’s productive layer
Authors: Marra John F. , Chamberlin W. Sean , Knudson Carol A. , Rhea W. Joseph , Ho Cheng
Published: April 2023

Here we compare various parameters that are used to define the depth of the ocean’s productive layer, the euphotic zone, during the initiation of the spring phytoplankton bloom in the North Atlantic (47°N/20°W). These are (1) the compensation depth (Zc, where gross photosynthesis balances autotrophic respiration), (2) the depth horizon of 1% of surface photosynthetic active irradiance (Ed(0-,PAR), (3) depth horizon of 1% of surface Ed(488), and (4) the depth of the bottom of the fluorescence maximum. We also use two related parameters, the depth of the mixed layer and the ratio of integral gross production to integral respiration as a scaling factor for a proxy for the critical depth. Over the course of the observational period (25 April - 7 May, 1989), the mixed layer decreased from 162 to 20 m, and Zc decreased from 64 to 35 m. The depth of 1%Ed(0-,PAR) followed the trajectory of Zc, while Ed(488) and the depth of the bottom of the fluorescence maximum were about 10 m deeper, on average. These data support the criterion of the depth of “1%PAR” to describe the productive layer for more productive areas of the ocean. However, 1% of Ed(488) or the bottom of the fluorescence maximum could be used over a broader range of trophic conditions.
Predicting the regime shift of coastal wetlands based on the bistability features in the intertidal zone: A case study in the Liaohe estuary
Authors: Jia Xin , Yang Juan , Wang Chen , Liu Baolin , Zheng HouYi , Zou Yu , Wang Heng , Zhao Huan
Published: April 2023

Influenced by human activities and natural interference, the worldwide distribution of coastal wetlands is now undergoing rapid evolution. The prediction on the locations of vegetation conversion is greatly important for the management of these coastal ecosystems in terms of early warning. In this paper, a series of waterlines extracted from multiple satellite images were used to generate a high-precision digital elevation model (DEM) in the intertidal zone of the Liaohe estuary. Based on the characteristics of the alternative stable states in elevation and normalized difference vegetation index (NDVI), the Logistic Regression Model was applied to predict the potential locations of vegetation expansion with geomorphic factors, such as elevation, slope, and annual changing rate of elevation. Before the prediction, the existence of two stable states in the landscape was confirmed in the study area, i.e., low-lying tidal flats and high-lying saltmarshes. When the geomorphic parameters exceeded the thresholds, the stable state transition would occur. By using the Logistic Regression Model, the elevation was the best explainer for determining the vegetation conversion in the single-factor simulation, while the slope was the worst. When multiple factors were integrated in simulations, the prediction with the elevation, slope, and annual elevation change rate was the best, with R2 = 0.739, and the overall accuracy of prediction reached 88.6%. The prediction result indicated that the area of saltmarshes in the Liaohe estuary increased by 16.7 km2 at a rate of 0.8% per year between 2011 and 2015. When considering the popularization in restoration practice, it is necessary to evaluate the reliability and flexibility of the Logistic Regression Model in predicting vegetation conversion in more types of estuarine wetlands.
Geographical subdivision of Alviniconcha snail populations in the Indian Ocean hydrothermal vent regions
Authors: Jang Sook-Jin , Cho Soo-Yeon , Li Chuyu , Zhou Yadong , Wang Hui , Sun Jin , Patra Ajit Kumar , Won Yong-Jin
Published: April 2023

The hairy snails of the genusAlviniconchaare representative deep-sea hydrothermal vent animals distributed across the Western Pacific and Indian Ocean. Out of six known species in the genusAlviniconcha, only one nominal species ofA. marisindicawas found in the Indian Ocean from the Carlsberg Ridge (CR), Central Indian Ridge (CIR) to the northern part of Southwest Indian Ridge (SWIR) and Southeast Indian Ridge (SEIR). Recently, theAlviniconchasnails were found at three new vent fields, named Onnare, Onbada, and Onnuri, in the northern CIR, which promotes a more comprehensive phylogeographic study of this species. Here, we examined the phylogeography and connectivity of theAlviniconchasnails among seven vent fields representing the CR and CIR based on DNA sequence data of a mitochondrialCOIgene and two protein-coding nuclear genes. Phylogenetic inferences revealed that theAlviniconchasnails of the newly found in the northern CIR and two vent fields of Wocan and Tianxiu in the CR were divergent with the previously identifiedA. marisindicain the southern CIR and mitochondrialCOIdata supported the divergence with at least greater than 3% sequence divergence. Population structure analyses based on the three genetic markers detected a phylogeographic boundary between Onnuri and Solitaire that divides the whole snail populations into northern and southern groups with a low migration rate. The high degree of genetic disconnection around the ‘Onnuri’ boundary suggests that theAlviniconchasnails in the Indian Ocean may undergo allopatric speciation. The border may similarly act as a dispersal barrier to many other vent species co-distributed in the CIR. This study would expand understanding the speciation and connectivity of vent species in the Indian Ocean.
Interface structure between Nb thin film and MgO(112) substrate: A first-principles prediction
Authors: Yang Jun , Moh Pak Yan , Baco Saturi , Jin Yongzhong , Fang Yong , Zong Hongxiang
Published: April 2023

The crystal orientation of ceramic substrates is an important factor affecting the interface structure of metal/ceramic composite materials. However, there is little information about the interface composed of metal films and ceramic substrates with a high-index plane. In this work, we predicted the interface structure between a Nb film and a MgO(112) substrate by calculating the interface separation works of different interface models by using the first-principles calculation method. The results showed that the preferred growth direction is Nb [120], and that the value of the interface separation work is 0.35 eV/Å2. The lattice mismatch between the film and substrate is less than 3%, implying that a coherent interface type is highly realizable in Nb/MgO(112). Furthermore, we analyzed the interface structures of Nb/MgO(100), Nb/MgO(110), Nb/MgO(111), and Nb/MgO(112) and found that the unique atomic configuration of the MgO substrate is the main factor determining the preferred interface structure of Nb/MgO.
Interleukins, growth factors, and transcription factors are key targets for gene therapy in osteoarthritis: A scoping review
Authors: Uebelhoer Melanie , Lambert Cécile , Grisart Juliane , Guse Kilian , Plutizki Stanislav , Henrotin Yves
Published: April 2023

Objective

Osteoarthritis (OA) is the most common degenerative joint disease, characterized by a progressive loss of cartilage associated with synovitis and subchondral bone remodeling. There is however no treatment to cure or delay the progression of OA. The objective of this manuscript was to provide a scoping review of the preclinical and clinical studies reporting the effect of gene therapies for OA.

Method

This review followed the JBI methodology and was reported in accordance with the PRISMA-ScR checklist. All research studies that explore in vitro, in vivo, or ex vivo gene therapies that follow a viral or non-viral gene therapy approach were considered. Only studies published in English were included in this review. There were no limitations to their date of publication, country of origin, or setting. Relevant publications were searched in Medline ALL (Ovid), Embase (Elsevier), and Scopus (Elsevier) in March 2023. Study selection and data charting were performed by two independent reviewers.

Results

We found a total of 29 different targets for OA gene therapy, including studies examining interleukins, growth factors and receptors, transcription factors and other key targets. Most articles were on preclinical in vitro studies (32 articles) or in vivo animal models (39 articles), while four articles were on clinical trials related to the development of TissueGene-C (TG-C).

Conclusion

In the absence of any DMOAD, gene therapy could be a highly promising treatment for OA, even though further development is required to bring more targets to the clinical stage.
Identifying microbial signatures for patients with postmenopausal osteoporosis using gut microbiota analyses and feature selection approaches
Authors: Huang Dageng , Wang Jihan , Zeng Yuhong , Li Qingmei , Wang Yangyang
Published: April 2023

Osteoporosis (OP) is a metabolic bone disorder characterized by low bone mass and deterioration of micro-architectural bone tissue. The most common type of OP is postmenopausal osteoporosis (PMOP), with fragility fractures becoming a global burden for women. Recently, the gut microbiota has been connected to bone metabolism. The aim of this study was to characterize the gut microbiota signatures in PMOP patients and controls. Fecal samples from 21 PMOP patients and 37 controls were collected and analyzed using amplicon sequencing of the V3-V4 regions of the 16S rRNA gene. The bone mineral density (BMD) measurement and laboratory biochemical test were performed on all participants. Two feature selection algorithms, maximal information coefficient (MIC) and XGBoost, were employed to identify the PMOP-related microbial features. Results showed that the composition of gut microbiota changed in PMOP patients, and microbial abundances were more correlated with total hip BMD/T-score than lumbar spine BMD/T-score. Using the MIC and XGBoost methods, we identified a set of PMOP-related microbes; a logistic regression model revealed that two microbial markers (Fusobacteria and Lactobacillaceae) had significant abilities in disease classification between the PMOP and control groups. Taken together, the findings of this study provide new insights into the etiology of OP/PMOP, as well as modulating gut microbiota as a therapeutic target in the diseases. We also highlight the application of feature selection approaches in biological data mining and data analysis, which may improve the research in medical and life sciences.
Sensory innervation of the lumbar 5/6 intervertebral disk in mice
Authors: Chen Lunhao , Lu Xuan , Jin Qianjun , Gao Zhihua , Wang Yue
Published: April 2023

Introduction

Over the years, most back pain-related biological studies focused on the pathogenesis of disk degeneration. It is known that nerve distributions at the outer layer of the annulus fibrosus (AF) may be an important contributor to back pain symptoms. However, the types and origins of sensory nerve terminals in the mouse lumbar disks have not been widely studied. Using disk microinjection and nerve retrograde tracing methods, the current study aimed to characterize the nerve types and neuropathway of the lumbar 5/6 (L5/6) disk in mice.

Methods

Using an anterior peritoneal approach, the L5/6 disk of adult C57BL/6 mice (males, 8–12 weeks) disk microinjection was performed. Fluorogold (FG) was injected into the L5/6 disk using the Hamilton syringe with a homemade glass needle driven by a pressure microinjector. The lumbar spine and bilateral thoracic 13 (Th13) to L6 DRGs were harvested at 10 days after injection. The number of FG+ neurons among different levels was counted and analyzed. Different nerve markers, including anti-neurofilament 160/200 (NF160/200), anti-calcitonin gene-related peptide (CGRP), anti-parvalbumin (PV), and anti-tyrosine hydroxylase (TH), were used to identify different types of nerve terminals in AF and their origins in DRG neurons.

Results

There were at least three types of nerve terminals at the outer layer of L5/6 AF in mice, including NF160/200+ (indicating Aβ fibers), CGRP+ (Aδ and C fibers), and PV+ (proprioceptive fibers). No TH+ fibers (sympathetic nerve fibers and some C-low threshold mechanoreceptors) were noticed in either. Using retrograde tracing methods, we found that nerve terminals in the L5/6 disk were multi-segmentally from Th13-L6 DRGs, with L1 and L5 predominately. An immunofluorescence analysis revealed that FG+ neurons in DRGs were co-localized with NF160/200, CGRP, and PV, but not TH.

Conclusion

Intervertebral disks were innervated by multiple types of nerve fibers in mice, including Aβ, Aδ, C, and proprioceptive fibers. No sympathetic nerve fibers were found in AF. The nerve network of the L5/6 disk in mice was multi-segmentally innervated by the Th13-L6 DRGs (mainly L1 and L5 DRGs). Our results may serve as a reference for preclinical studies of discogenic pain in mice.
Solving inherited white matter disorder etiologies in the neurology clinic: Challenges and lessons learned using next-generation sequencing
Authors: Perrier Stefanie , Guerrero Kether , Tran Luan T. , Michell-Robinson Mackenzie A. , Legault Geneviève , Brais Bernard , Sylvain Michel , Dorman James , Demos Michelle , Köhler Wolfgang , Pastinen Tomi , Thiffault Isabelle , Bernard Geneviève
Published: April 2023

Introduction

Rare neurodevelopmental disorders, including inherited white matter disorders or leukodystrophies, often present a diagnostic challenge on a genetic level given the large number of causal genes associated with a range of disease subtypes. This study aims to demonstrate the challenges and lessons learned in the genetic investigations of leukodystrophies through presentation of a series of cases solved using exome or genome sequencing.

Methods

Each of the six patients had a leukodystrophy associated with hypomyelination or delayed myelination on MRI, and inconclusive clinical diagnostic genetic testing results. We performed next generation sequencing (case-based exome or genome sequencing) to further investigate the genetic cause of disease.

Results

Following different lines of investigation, molecular diagnoses were obtained for each case, with patients harboring pathogenic variants in a range of genes including TMEM106B, GJA1, AGA, POLR3A, and TUBB4A. We describe the lessons learned in reaching the genetic diagnosis, including the importance of (a) utilizing proper multi-gene panels in clinical testing, (b) assessing the reliability of biochemical assays in supporting diagnoses, and (c) understanding the limitations of exome sequencing methods in regard to CNV detection and region coverage in GC-rich areas.

Discussion

This study illustrates the importance of applying a collaborative diagnostic approach by combining detailed phenotyping data and metabolic results from the clinical environment with advanced next generation sequencing analysis techniques from the research environment to increase the diagnostic yield in patients with genetically unresolved leukodystrophies.
Clinical impacts of the concomitant use of L-asparaginase and total parenteral nutrition containing L-aspartic acid in patients with acute lymphoblastic leukemia
Authors: Ko Minoh , Kim Myeong Gyu , Yoon Sung-Soo , Kim In-Wha , Suh Sung Yun , Cho Yoon-Sook , Oh Jung Mi
Published: April 2023

Introduction

L-asparaginase (ASNase) depletes L-asparagine and causes the death of leukemic cells, making it a mainstay for the treatment of acute lymphoblastic leukemia (ALL). However, ASNase's activity can be inhibited by L-aspartic acid (Asp), which competes for the same substrate and reduces the drug's efficacy. While many commercially used total parenteral nutrition (TPN) products contain Asp, it is unclear how the concomitant use of TPNs containing Asp (Asp-TPN) affects ALL patients treated with ASNase. This propensity-matched retrospective cohort study evaluated the clinical effects of the interaction between ASNase and Asp-TPN.

Methods

The study population included newly diagnosed adult Korean ALL patients who received VPDL induction therapy consisting of vincristine, prednisolone, daunorubicin, and Escherichia coli L-asparaginase between 2004 and 2021. Patients were divided into two groups based on their exposure to Asp-TPN: (1) Asp-TPN group and (2) control group. Data, including baseline characteristics, disease information, medication information, and laboratory data, were collected retrospectively. The primary outcomes for the effectiveness were overall and complete response rates. Relapse-free survival at six months and one year of treatment were also evaluated. The safety of both TPN and ASNase was evaluated by comparing liver function test levels between groups. A 1:1 propensity score matching analysis was conducted to minimize potential selection bias.

Results

The analysis included a total of 112 ALL patients, and 34 of whom received Asp-TPN and ASNase concomitantly. After propensity score matching, 30 patients remained in each group. The concomitant use of Asp-TPN and ASNase did not affect the overall response rate (odds ratio [OR] 0.53; 95% confidence interval [CI] = 0.17–1.62) or the complete response rate (OR 0.86; 95% CI = 0.29–2.59) of the ASNase-including induction therapy. The concomitant use of Asp-TPN and ASNase also did not impact relapse-free survival (RFS) at six months and one year of treatment (OR 1.00; 95% CI = 0.36–2.78 and OR 1.24; 95% CI, 0.50–3.12, respectively). The peak levels of each liver function test (LFT) and the frequency of LFT elevations were evaluated during induction therapy and showed no difference between the two groups.

Conclusion

There is no clear rationale for avoiding Asp-TPN in ASNase-treated patients.
Next-generation sequencing study on poorly differentiated carcinoma derived from a thirty-year-old epidermoid cyst: A case report
Authors: Choi Woosuk , Park Joseph Kyu-hyung , Song Seung Geun , Kim Baek-kyu
Published: April 2023

Although epidermoid cysts are frequently seen as benign lesions, they are highly uncommon to develop into cancerous lesions. A 36-year-old man with a cystic mass present on his left flank since childhood presented to our department. Based on the patient’s medical history and abdominal computed tomography scan, we excised the lesion under the suspicion of an epidermoid cyst. Histopathological evaluation revealed the presence of poorly differentiated carcinoma with squamoid and basaloid differentiation, which showed a strong possibility of carcinoma arising from an epidermal cyst. Next-generation sequencing using TruSight oncology 500 assay showed copy number variation of ATM and CHEK1 genes.
Telomere dysfunction in some pediatric congenital and growth-related diseases
Authors: Zheng Bo , Fu Jianhua
Published: April 2023

Telomere wear and dysfunction may lead to aging-related diseases. Moreover, increasing evidence show that the occurrence, development, and prognosis of some pediatric diseases are also related to telomere dysfunction. In this review, we systematically analyzed the relationship between telomere biology and some pediatric congenital and growth-related diseases and proposed new theoretical basis and therapeutic targets for the treatment of these diseases.
Hypolipidemic and insulin sensitizing effects of salsalate beyond suppressing inflammation in a prediabetic rat model
Authors: Hüttl Martina , Markova Irena , Miklánková Denisa , Zapletalova Iveta , Kujal Petr , Šilhavý Jan , Pravenec Michal , Malinska Hana
Published: April 2023

Background and aims: Low-grade chronic inflammation plays an important role in the pathogenesis of metabolic syndrome, type 2 diabetes and their complications. In this study, we investigated the effects of salsalate, a non-steroidal anti-inflammatory drug, on metabolic disturbances in an animal model of prediabetes—a strain of non-obese hereditary hypertriglyceridemic (HHTg) rats.

Materials and Methods: Adult male HHTg and Wistar control rats were fed a standard diet without or with salsalate delivering a daily dose of 200 mg/kg of body weight for 6 weeks. Tissue sensitivity to insulin action was measured ex vivo according to basal and insulin-stimulated 14C-U-glucose incorporation into muscle glycogen or adipose tissue lipids. The concentration of methylglyoxal and glutathione was determined using the HPLC-method. Gene expression was measured by quantitative RT-PCR.

Results: Salsalate treatment of HHTg rats when compared to their untreated controls was associated with significant amelioration of inflammation, dyslipidemia and insulin resistance. Specificaly, salsalate treatment was associated with reduced inflammation, oxidative and dicarbonyl stress when inflammatory markers, lipoperoxidation products and methylglyoxal levels were significantly decreased in serum and tissues. In addition, salsalate ameliorated glycaemia and reduced serum lipid concentrations. Insulin sensitivity in visceral adipose tissue and skeletal muscle was significantly increased after salsalate administration. Further, salsalate markedly reduced hepatic lipid accumulation (triglycerides −29% and cholesterol −14%). Hypolipidemic effects of salsalate were associated with differential expression of genes coding for enzymes and transcription factors involved in lipid synthesis (Fas, Hmgcr), oxidation (Pparα) and transport (Ldlr, Abc transporters), as well as changes in gene expression of cytochrome P450 proteins, in particular decreased Cyp7a and increased Cyp4a isoforms.

Conclusion: These results demonstrate important anti-inflammatory and anti-oxidative effects of salsalate that were associated with reduced dyslipidemia and insulin resistance in HHTg rats. Hypolipidemic effects of salsalate were associated with differential expression of genes regulating lipid metabolism in the liver. These results suggest potential beneficial use of salsalate in prediabetic patients with NAFLD symptoms.
Genomic and genetic advances of oiltea-camellia (Camellia oleifera)
Authors: Ye Changrong , He Zhilong , Peng Jiayu , Wang Rui , Wang Xiangnan , Fu Mengjiao , Zhang Ying , Wang Ai , Liu Zhixian , Jia Gaofeng , Chen Yongzhong , Tian Bingchuan
Published: April 2023

Oiltea-camellia (C. oleifera) is a widely cultivated woody oil crop in Southern China and Southeast Asia. The genome of oiltea-camellia was very complex and not well explored. Recently, genomes of three oiltea-camellia species were sequenced and assembled, multi-omic studies of oiltea-camellia were carried out and provided a better understanding of this important woody oil crop. In this review, we summarized the recent assembly of the reference genomes of oiltea-camellia, genes related to economic traits (flowering, photosynthesis, yield and oil component), disease resistance (anthracnose) and environmental stress tolerances (drought, cold, heat and nutrient deficiency). We also discussed future directions of integrating multiple omics for evaluating genetic resources and mining key genes of important traits, and the application of new molecular breeding and gene editing technologies to accelerate the breeding process of oiltea-camellia.
Changes and transcriptome regulation of endogenous hormones during somatic embryogenesis in Ormosia henryi Prain
Authors: Wu Gaoyin , Wei Xiaoli , Wang Xiao , Wei Yi
Published: April 2023

Introduction

Ormosia henryi is a rare and endangered plant growing in southern China. Somatic embryo culture is an effective measure for the rapid propagation of O. henryi. It has not been reported how regulatory genes induce somatic embryogenesis by regulating endogenous hormone changes during the process of somatic embryogenesis in O. henryi.

Methods

In this study, we analysed the endogenous hormone levels and transcriptome data of nonembryogenic callus (NEC), embryogenic callus (EC), globular embryo (GE) and cotyledon embryo (CE) in O. henryi.

Results

The results showed that the indole-3-acetic acid (IAA) content was higher and the cytokinins (CKs) content was lower in EC than in NEC, and the gibberellins (GAs) and abscisic acid (ABA) contents were significantly higher in NEC than in EC. The contents of IAA, CKs, GAs and ABA increased significantly with EC development. The expression patterns of differentially expressed genes (DEGs) involved in the biosynthesis and signal transduction of auxin (AUX) (YUCCA and SAUR), CKs (B-ARR), GAs (GA3ox, GA20ox, GID1 and DELLA) and ABA (ZEP, ABA2, AAO3, CYP97A3, PYL and ABF) were consistent with the levels of endogenous hormones during somatic embryogenesis (SE). In this study, 316 different transcription factors (TFs) regulating phytohormones were detected during SE. AUX/IAA were downregulated in the process of EC formation and GE differentiation into CE, but other TFs were upregulated and downregulated.

Conclusion

Therefore, we believe that relatively high IAA content and low CKs, GAs and ABA contents contribute to EC formation. The differential expression of AUX, CKs, GAs and ABA biosynthesis and signal transduction genes affected the endogenous hormone levels at different stages of SE in O. henryi. The downregulated expression of AUX/IAA inhibited NEC induction, promoted EC formation and GE differentiation into CE.
Insight into the biochemical and physiological mechanisms of nanoparticles-induced arsenic tolerance in bamboo
Authors: Emamverdian Abolghassem , Ding Yulong , Hasanuzzaman Mirza , Barker James , Liu Guohua , Li Yang , Mokhberdoran Farzad
Published: April 2023

Introduction

Arsenic (As) contamination in soil, sediments, and water poses a significant threat to the growth of bamboo plants. However, nanoparticles with high metal absorbance capacity can play a key role in the reduction of heavy metals toxicity in plants as well as maintaining their growth under toxicity.

Methods

Hence, an in vitro experiment was conducted to determine the influence of three types of nanoparticles: 150 µM silicon nanoparticles (SiO2 NPs), 150 µM titanium nanoparticles (TiO2 NPs), and 150 µM zinc oxide nanoparticles (ZnO NPs) on As (150 µM and 250 µM) tolerance enhancement of a one-year-old bamboo species (Pleioblastus pygmaeus).

Results and discussion

The results showed that while As at 150 µM and 250 µM significantly disrupted the plant growth by excessive generation of reactive oxygen species (ROS) components, and inducing cell membrane peroxidation, the addition of NPs increased both enzymatic and non-enzymatic antioxidant activities, upregulated glyoxalase defense system, and improved gas exchange parameters and photosynthetic pigments content, leading to the enhanced plant shoot and root dry weight. These were achieved by lowering levels of ROS, electrolyte leakage (EL), malondialdehyde (MDA), hydrogen peroxide (H2O2) and the superoxide radical (O2•−), as well as decreasing As accumulation in the plant organs. Thus, it might be concluded that ZnO NPs, SiO2NPs, and TiO2NPS alone or in combination can significantly increase the bamboo plant tolerance to As toxicity via key mechanisms, including induction of various antioxidants and glyoxalase defense systems, scavenging of ROS and methylglyoxal (MG), increasing phytochelatins production, reduction of As accumulation and translocation, and improving photosynthetic pigments under As toxicity. Additionally, the results showed that the combined application of 150 µM ZnO NPs, SiO2 NPs, and TiO2 NPs had the greatest effect on enhancing the plant tolerance to As at 150 µM and 250 µM.
Impact of irrigation water quality on human norovirus surrogate survival during leafy green production
Authors: Wu Xi , Moyne Anne-laure , Ramos Thais De Melo , Harris Linda J. , DiCaprio Erin
Published: April 2023

Introduction

The impact of water quality on the survival of human norovirus (NoV) was determined in irrigation water field run-off (tail water) and well water from a representative Central Coast vegetable production site in the Salinas Valley, California.

Methods

Tail water, well water, and ultrapure water samples were inoculated separately with two surrogate viruses for human NoV—Tulane virus (TV) and murine norovirus (MNV)—to achieve a titer of 1×105 plaque forming units (PFU)/ml. Samples were stored at 11, 19, and 24°C for 28 days. Additionally, inoculated water was applied to soil collected from a vegetable production site in the Salinas Valley or to the surface of growing romaine lettuce leaves, and virus infectivity was evaluated for 28 days in a growth chamber.

Results

Virus survival was similar for water stored at 11, 19, and 24°C and there was no difference in infectivity based on water quality. After 28 days, a maximum 1.5 log reduction was observed for both TV and MNV. TV decreased by 1.97-2.26 log and MNV decreased by 1.28- 1.48 logs after 28 days in soil; infectivity was not influenced by water type. Infectious TV and MNV were recovered from lettuce surfaces for up to 7 and 10 days after inoculation, respectively. Across the experiments there was no significant impact of water quality on the stability of the human NoV surrogates.

Discussion

Overall, the human NoV surrogates were highly stable in water with a less than 1.5 log reduction over 28 days and no difference observed based on the water quality. In soil, the titer of TV declined by approximately 2 logs over 28 days, while MNV declined by 1 log during the same time interval, suggesting surrogate-specific inactivation dynamics in the soil tested in this study. A 5-log reduction in MNV (day 10 post inoculation) and TV (day 14 post inoculation) was observed on lettuce leaves, and the inactivation kinetics were not significantly impacted by the quality of water used. These results suggest that human NoV would be highly stable in water, and the quality of the water (e.g., nutrient content, salinity, and turbidity) does not significantly impact viral infectivity.
Genome-edited HEADING DATE 3a knockout enhances leaf production in Perilla frutescens
Authors: Yun Hee Rang , Chen Chong , Kim Jee Hye , Kim Hae Eun , Karthik Sivabalan , Kim Hye Jeong , Chung Young-Soo , Baek Hee Soon , Sung Sibum , Kim Hyun Uk , Heo Jae Bok
Published: April 2023

Environmental cues regulate the transition of many plants from vegetative to flowering development. Day length, or photoperiod, is one cue that synchronizes flowering by changing seasons. Consequently, the molecular mechanism of flowering control is prominent in Arabidopsis and rice, where essential genes like FLOWERING LOCUS T (FT) homolog, HEADING DATE 3a (Hd3a), have been connected to flowering regulation. Perilla is a nutrient-rich leaf vegetable, and the flowering mechanism remains largely elusive. We identified flowering-related genes under short-day conditions using RNA sequencing to develop an enhanced leaf production trait using the flowering mechanism in the perilla. Initially, an Hd3a-like gene was cloned from the perilla and defined as PfHd3a. Furthermore, PfHd3a is highly rhythmically expressed in mature leaves under short-day and long-day conditions. Ectopic expression of PfHd3a in Atft-1 mutant plants has been shown to complement Arabidopsis FT function, resulting in early flowering. In addition, our genetic approaches revealed that overexpression of PfHd3a in perilla caused early flowering. In contrast, the CRISPR/Cas9 generated PfHd3a-mutant perilla showed significantly late flowering, resulting in approximately 50% leaf production enhancement compared to the control. Our results suggest that PfHd3a plays a vital role in regulating flowering in the perilla and is a potential target for molecular breeding in the perilla.
The core triacylglycerol toolbox in woody oil plants reveals targets for oil production bioengineering
Authors: Cao Yunpeng , Li Qiang , Zhang Lin
Published: April 2023

Woody oil plants are the most productive oil-bearing species that produce seeds with high levels of valuable triacylglycerols (TAGs). TAGs and their derivatives are the raw materials for many macromolecular bio-based products, such as nylon precursors, and biomass-based diesel. Here, we identified 280 genes encoding seven distinct classes of enzymes (i.e., G3PAT, LPAAT, PAP, DGAT, PDCT, PDAT, and CPT) involved in TAGs-biosynthesis. Several multigene families are expanded by large-scale duplication events, such as G3PATs, and PAPs. RNA-seq was used to survey the expression profiles of these TAG pathway-related genes in different tissues or development, indicating functional redundancy for some duplicated genes originated from the large-scale duplication events, and neo-functionalization or sub-functionalization for some of them. Sixty-two genes showed strong, preferential expression during the period of rapid seed lipid synthesis, suggesting that their might represented the core TAG-toolbox. We also revealed for the first time that there is no PDCT pathway in Vernicia fordii and Xanthoceras sorbifolium. The identification of key genes involved in lipid biosynthesis will be the foundation to plan strategies to develop woody oil plant varieties with enhanced processing properties and high oil content.
Oil content analysis of corn seeds using a hand-held Raman spectrometer and spectral peak decomposition algorithm
Authors: Jin Yuan , Tian Hongwu , Gao Zhen , Yang Guiyan , Dong Daming
Published: April 2023

Rapid, non-destructive and reliable detection of the oil content of corn seeds is important for development of high-oil corn. However, determination of the oil content is difficult using traditional methods for seed composition analysis. In this study, a hand-held Raman spectrometer was used with a spectral peak decomposition algorithm to determine the oil contents of corn seeds. Mature and waxy Zhengdan 958 corn seeds and mature Jingke 968 corn seeds were analyzed. Raman spectra were obtained in four regions of interest in the embryo of the seed. After analysis of the spectra, a characteristic spectral peak for the oil content was identified. A Gaussian curve fitting spectral peak decomposition algorithm was used to decompose the characteristic spectral peak of oil at 1657 cm−1. This peak was used to determine the Raman spectral peak intensity for the oil content in the embryo and differences in the oil contents among seeds of varying maturity and different varieties. This method is feasible and effective for detection of corn seed oil.
Self-construal priming modulates sonic seasoning
Authors: Xu Jingxian , Guo Xiyu , Liu Mengying , Xu Hui , Huang Jianping
Published: April 2023

Introduction

“Sonic seasoning” is when music influences the real taste experiences of consumers. “Self-construal” is how individuals perceive, understand, and interpret themselves. Numerous studies have shown that independent and interdependent self-construal priming can affect a person's cognition and behavior; however, their moderating effect on the sonic seasoning effect remains unclear.

Methods

This experiment was a 2 (self-construal priming: independent self-construal or interdependent self-construal) × 2 (chocolate: milk chocolate or dark chocolate) × 2 (emotional music: positive emotional music or negative emotional music) mixed design, and explored the moderating role of self-construal priming and the effect of emotional music on taste by comparing participants' evaluations of chocolates while listening to positive or negative music after different levels of self-construal priming.

Results

After initiating independent self-construal, participants increased their ratings of milk chocolate sweetness when listening to music that elicited positive emotions, t(32) = 3.11, p = 0.004, Cohen's d = 0.54, 95% CI = [0.33, 1.61]. In contrast, interdependent self-construal priming led participants to perceive dark chocolate as sweeter when they heard positive music, t(29) = 3.63, p = 0.001, Cohen's d = 0.66, 95%CI = [0.44, 1.56].

Discussion

This study provides evidence for improving people's individual eating experience and enjoyment of food.
Cross-group friendship and collective action in community solidarity initiatives with displaced people and resident/nationals
Authors: Vine Megan , Greenwood Ronni Michelle
Published: April 2023

Introduction: In Ireland, people seeking asylum (displaced people) receive accommodation in a system called “Direct Provision” (DP) while they wait for their applications for protection to be processed. The living conditions of DP have been described as illegal and inhumane by national and international human rights groups, and the system exacerbates the social exclusion of displaced people. Community responses to DP by displaced people and resident/nationals of Ireland include the creation of informal groups called community solidarity initiatives (CSI), through which cross-group friendships are forged by participation in shared cultural activities. We hypothesized that, compared to non-CSI participants, participants of CSI would report more cross-group friendships, and that more cross-group friendships would predict stronger collective action intentions to support the campaign to end DP, especially among resident/nationals.

Methods: We recruited residents/nationals and displaced persons with and without CSI experience to complete a self-report questionnaire (n = 199), measuring cross-group friendship, collective action intentions, and intergroup attitudes. Data were collected between July 2020 and March 2021, using a combination of online and paper surveys. We conducted ANOVA and conditional process analyses on the data to test our hypotheses.

Results: As predicted, CSI participants reported more contact with cross-group friends and stronger collective action intentions than non-participators. Conditional process analysis indicated that CSI participation facilitated resident/nationals’ political solidarity with displaced people through cross-group friendship.

Discussion: Findings identify the role of group membership in the relationship between contact and collective action for migrant justice, illustrating the potential of CSI to bolster intergroup solidarity and social cohesion through shared activities and cross-group friendship. As such, findings make an important contribution to the literature on intergroup contact, solidarity, and social cohesion, and will be relevant for community practitioners, civil society organisations, NGOs, and policy makers.
The effect of the time parents spend with children on children's well-being
Authors: Li Dongxu , Guo Xi
Published: April 2023

Background

The time spent with parents is a crucial factor in the growth of children, and children's well-being is an important indicator of their mental health.

Methods

To promote the children's well-being, this study, which is relying on the data from the 2017 China Time Use Survey (CTUS), explores the relationship between parental time and children's well-being and specific influencing factors.

Results

The more time parents spent with children, the higher their children's well-being will be (coefficient 0.1020, p < 0.01). The life and leisure time parents spent with children promoted children's well-being (coefficient 0.1020, p < 0.01). The life and leisure time the mother spent with children (coefficient 0.1030, p < 0.05) the life and leisure time (coefficient 0.1790, p < 0.05) and the educational interactions time the father spent with children (coefficient 0.3630, p < 0.10) positively affected children's well-being. The influence of the time parents spent with children on children's well-being was heterogeneous based on their children's academic performance.

Conclusions

Parental accompaniment is an important determinant of children's well-being. Family education, guidance services, and mental health services should be strengthened, and it is necessary to improve the time spent with children and to pay attention to individual differences in children.
Mobile-assisted academic vocabulary learning with digital flashcards: Exploring the impacts on university students’ self-regulatory capacity
Authors: Boroughani Tahereh , Behshad Nastaran , Xodabande Ismail
Published: April 2023

With the global rise in international journals over the past decades, successful communication in science largely hinges upon developing competency in using English as the academic lingua franca. Accordingly, one aspect of developing academic literacy entails helping university students learn a group of medium-frequency and cross-disciplinary words (i.e., core academic vocabulary) employed extensively to describe abstract processes and organize rhetorical aspects of academic discourse. The current study aimed to investigate the contribution of mobile-assisted vocabulary learning with digital flashcards in scaffolding academic vocabulary learning and self-regulatory capacity development among university students. The participants were 54 Iranian university students selected based on their availability in the study context. The participants were assigned to an experimental group (N = 33) and a control learning condition (N = 21). Those in the experimental group used digital flashcards (i.e., Quizlet) to learn academic words in a recently developed core academic wordlist (i.e., NAWL), and the control group used traditional materials (wordlists) to learn the same vocabulary items. The participants’ vocabulary knowledge and self-regulatory capacity for vocabulary learning were tested before and after the treatments. The findings indicated that although both groups improved their vocabulary knowledge and self-regulatory capacity after 4 months, the experimental group outperformed the control group in both measures, and the effect sizes of the observed differences were very large. Consequently, the study provided empirical evidence for the effectiveness of mobile-assisted vocabulary learning over traditional materials in developing academic literacy. The findings also indicated that using digital flashcards for vocabulary learning improves university students’ capacity for undertaking self-regulated vocabulary learning. The implications of these findings for EAP programs are highlighted.
Widowhood, social networks, and mental health among Chinese older adults: The moderating effects of gender
Authors: Tang Dan , Mair Christine A. , Hu Qing
Published: April 2023

Objectives

This study aimed to examine the three-way interaction between widowhood, social ties, and gender and its effects on older adults’ mental health, including depressive symptoms and life satisfaction, in the context of China.

Methods

Participants were 7,601 Chinese older adults. Their social network was divided between family and friendship ties, and their mental health was measured by depressive symptoms and life satisfaction. Linear regression was employed to analyze the associations between widowhood, social networks, and mental health, as well as to explore the moderating effect of gender.

Results

Widowhood is associated with more depressive symptoms, but not with life satisfaction, while family and friendship ties are associated with less depressive symptoms and greater life satisfaction. Furthermore, the lack of family ties is associated with more depressive symptoms for widowed men compared to married older men, while it is associated with lower life satisfaction for widowed women compared to married older women.

Conclusion

Family ties are the most important social support resource for Chinese older adults, especially for the widowed group. The vulnerability of older widowed men who lack family ties in China deserves public concern and attention.
Demographic factors, partial social belonging and psychological resources associated with coping
Authors: Eshel Yohanan , Kimhi Shaul , Marciano Hadas , Adini Bruria
Published: April 2023

Introduction

The present study investigates the role of perceived partial social belonging (PPSB) in determining societal and individual resilience and positive and negative coping indicators. It is assumed that most people aspire to belong and be integrated into their society. A sense of only partial belonging is therefore distressing for them.

Methods

Two hypotheses are examined in the current study: (a) A higher level of PPSB will predict a lower level of resilience and a higher level of psychological symptoms. (b) PPSB will mediate the associations between three stress-evoking demographic characteristics (younger age, low income, and gender) and the lower psychological resilience and higher distress associated with these demographic characteristics. These hypotheses were examined using a sample of the Israeli Jewish public (N = 1,502) who responded to an anonymous questionnaire about the investigated issues. The data were collected by an internet panel company possessing a database of more than 65,000 residents, representing the varied components of the Israeli society.

Results

The findings supported our hypotheses: (a) PPSB negatively predicted societal and individual resilience and hope and positively predicted distress symptoms and sense of danger. (b) PPSB mediated the effects of the investigated demographic variables on these psychological variables.

Conclusion

These results are discussed in association with the concept of belonging competencies. Our findings display that being unsure about one’s belonging to a desired social group, has a major role in increasing psychological distress and sense of danger and in reducing hope and both individual and societal resilience.