bluuebunny/crossref_metadata_2025 huggingface dataset:
I Can Grow as an In Utero if You Give Me Enough Nutrition: Premature ≤29 Weeks Gestation
Authors: Hoyos Angela
Published: May 2021

Introduction: In general, everyone believe they should grow as “in utero” for the smallest premature babies but many thinks that it is not possible with today's nutrition “guidelines”. There is resistance to give enough nutrition for fear of "toxicity". We use volumes that some would consider high, although there are more and more groups that accept it. We chose the difference in Z-score between birth and a corrected discharge gestational age to assess postnatal growth in our unit.Material and methods: Between January 2018 and December 2020, we review all cases ≤ 29 weeks of GA at birth that survived to 36 weeks corrected gestational age, or that were discharged home if this occurred before. We had a protocol with extremely aggressive nutrition including parenteral as well as oral nutrition. We then separated the patients who had lost more than 1 Z-score point to see the weight trajectory plotted on the Fenton 2013 growth curve and to review the clinical characteristics of those patients. We also graphed the patients who had had a smaller Z-score difference to also see their trajectory in the Fenton curves. We calculate the number of cases, medians, and interquartile ranges for all groups.Results: We fount 32 cases. The median ± IQR (interquartile range) of change in Z-score between birth and discharge of the whole group was -0.52 ± 0.53; 6/32 (19%) had more than 1 point drop; all had severe diseases listed in Figure 1 with plotting all cases. The median decline in Z score ± IQR of this group with poor growth was 1.24 ± 0.22. The group of cases with a fall of <1 point of Z score were 26 (81%), the median ± IQR of fall of Z score was 0.39 ± 0.55 (see figure 2). All cases had the initial fall in weight the first 1-2 weeks. No important complications secondary to the ingested volumes or parenteral nutrition were reported. Conclusion: The group of cases with a fall of > 1 point of Z score had severe pathologies. The other cases had an adequate growth parallel to normal growth charts in some cases with some catch up.Discussion: With this work we try to show that in many premature babies it is possible to grow like in utero if they are given enough nutrition. Randomized studies are necessary to confirm our findings.
Crushing of Single-Walled Corrugated Board During Converting: Experimental and Numerical Study
Authors: Garbowski Tomasz , Gajewski Tomasz , Mrówczyński Damian , Jędrzejczak Radosław
Published: May 2021

Corrugated cardboard is an ecological material, mainly because, in addition to virgin cellulose fibers also the fibers recovered during recycling process are used in its production. However, the use of recycled fibers causes slight deterioration of the mechanical properties of the corrugated board. In addition, converting processes such as printing, die-cutting, lamination, etc. cause micro-damage in the corrugated cardboard layers. In this work, the focus is precisely on the crushing of corrugated cardboard. A series of laboratory experiments were conducted, in which the different types of single-walled corrugated cardboards were pressed in a fully controlled manner to check the impact of the crush on the basic material parameters. The amount of crushing (with a precision of 10 micrometers) was controlled by a precise FEMat device, for crushing the corrugated board in the range from 10 to 70 % of its original thickness. In this study, the influence of crushing on bending, twisting and shear stiffness as well as a residual thickness and edge crush resistance of corrugated board was investigated. Then, a procedure based on a numerical homogenization, taking into account a partial delamination in the corrugated layers to determine the degraded material stiffness was proposed. Finally, using the empirical-numerical method, a simplified calculation model of corrugated cardboard was derived, which satisfactorily reflects the experimental results.
Social Interactions of DAT-HET Epi-Genotypes Differing for Maternal Origins: The Development of a New Preclinical Model of Socio-Sexual Apathy
Authors: Brancato Anna , Lo Russo Sara , Liberati Anna Sara , Carbone Cristiana , Zelli Silvia , Laviola Giovanni , Cannizzaro Carla , Adriani Walter
Published: May 2021

Social interaction is essential for life and is impaired in many psychiatric disorders like schizophrenia, au-tism, depression and major anxiety disorder. Monoamine transmission plays a key role in social behavior and both genetic and epigenetic modifications of dopamine and noradrenaline neurotransmission-related genes can affect the levels of social interaction. Since heterozygous individuals for a specific genetic trait possess only one mutant allele of that trait, in order to better evaluate the role of the interaction between genetics and epigenetics in unmasking latent genetically-determined predispositions, our interest has focused on studying the interplay between genetics and epigenetics influences on social behavior in male rats obtained by two different breeding schemes: a first group by breeding of knock-out (KO) male rats with wild-type (WT) female dams (homogeneous heterozygous offspring, termed MAT-HET), and a second group of heterozygous DAT male offspring by breeding of KO male and DAT-heterozygous female subjects (to obtain comparable control pups, termed MIX-HET). Their social behavior was then assessed by partner preference, social preference and elicited preference tests. In the first test MIX-HET and MAT-HET male mice had choice between two WT females one in estrous and the other not in estrous. In the second test they met either a MIX-HET or a WT male rodent. Also, the expression of the noradrenaline transporter (NET) was assessed in the prefrontal cortex, hippocampus and hypothalamus of MAT, MIX and WTs by immunofluorescence in order to estimate its involvement in the expression of social behavior. Our results show that MIX-HET focal rodents tend to have an asocial behavior when in contact with a female in estrous, and their behavior is similar to when the stimulus is a MIX-HET male. MAT-HET male rodents, instead, tend to be very attracted by the female in estrous, but they ignore the MIX-HET stimulus. MIX-HET progeny showed a lower expression of noradrenaline transporter in both hypothalamus and hippocampus with respect to MAT-HET rats, whereas MAT-HET rats displayed increased noradrenaline transporter immunofluorescence in the hypothalamus and in the hippocampus with respect to WT rats, while no difference was observed in the prefrontal cortex. Therefore we can hypothesize that the differences observed between the two heterozygous groups may be attributable to an epigenetic factor: the different maternal care received. These data can open new perspectives towards increased the preclinical knowledge about autism and bipolar disorder.
Low-Cost Algorithms for Metabolic Pathway Pairwise Comparison
Authors: Arias-Méndez Esteban , Barquero-Morera Diego , Torres-Rojas Francisco J.
Published: May 2021

Metabolic pathways provide key information to achieve a better understanding of life and all its processes; this is useful information for the improvement of medicine, agronomy, pharmacy, and other similar areas. The main analysis tool used to study these pathways is based on the idea of pathway comparison, using graph data structures. Metabolic pathway comparison has been defined as a computationally complex task \cite{ay2011submap,abaka2013campways}. In previous work from 2017, two different approaches that simplify the problem of comparing pathways represented as graphs were introduced. The first algorithm consists of the transformation of a two-dimensional graph structure, representing a metabolic pathway, to a one-dimensional structure and thus aligning the corresponding data using a reduced 1 dimension string. The second algorithm consists of performing a paired analysis between reactions in pathways and thus eliminating all similarities, finally, showing these differences to the user. The suggestion is to use the information provided by these algorithms as a previous analysis to a deeper, more expensive, comparison tool use. Here we provide an extension of this work with more data and deeper analysis. These methods have shown to be an effective way to treat the problem of metabolic pathway comparison as listed in the discussion and results section. Our results show evidence of a quick, simple and effective way to resolve the described problem.
Dual Mode of Action of Acetylcholine on Cytosolic Calcium Oscillations in Pancreatic Beta and Acinar Cells in Situ
Authors: Sluga Nastja , Postic Sandra , Sarikas Srdjan , Huang Ya-Chi , Stožer Andraz , Slak Rupnik Marjan
Published: May 2021

Cholinergic innervation in pancreas controls both the release of digestive enzymes to support the intestinal digestion and absorption, as well as insulin release to promote nutrient use in the cells of the body. The effects of muscarinic receptor stimulation are described in detail for endocrine beta cells and exocrine acinar cells separately. Here we describe morphological and functional criteria to separate these two cell types in situ in tissue slices and simultaneously measure their response to ACh stimulation on cytosolic Ca2+ oscillations [Ca2+]c in stimulatory glucose conditions. Our results show that both cell types respond to glucose directly in the concentration range compatible with the glucose transporters they express. The physiological ACh concentration increases the frequency of glucose stimulated [Ca2+]c oscillations in both cell types and synchronizes [Ca2+]c oscillations in acinar cells. The pharmacological ACh concentration further increases the oscillation frequency on the level of individual beta cells, inhibits the synchronization between these cells, and abolishes oscillatory activity in acinar cells. We discuss possible mechanisms leading to the observed phenomena.
Crosslinking Strategies for Microfluidic Production of Microgels
Authors: Chen Minjun , Bolognesi Guido , Vladisavljević Goran T.
Published: May 2021

This article provides a systematic review of the crosslinking strategies used to produce microgel particles in microfluidic chips. Various ionic crosslinking methods for gelation of charged pol-ymers are discussed, including external gelation via crosslinkers dissolved or dispersed in the oil phase, internal gelation methods using crosslinkers added to the dispersed phase in their non-active forms, such as chelating agents, photo-acid generators, sparingly soluble or slowly hydrolyzing compounds, and methods involving competitive ligand exchange, rapid mixing of polymer and crosslinking streams, and merging polymer and crosslinker droplets. Covalent crosslinking methods using enzymatic oxidation of modified biopolymers, photo-polymerization of crosslinkable monomers or polymers, and thiol-ene “click” reactions are also discussed, as well as the methods based on sol-gel transitions of stimuli responsive polymers triggered by pH or temperature change. In addition to homogeneous microgel particles, the production of structurally heterogeneous particles such as composite hydrogel particles entrapping droplet interface bi-layers, core-shell particles, organoids, and Janus particles are also discussed. Microfluidics offers the ability to precisely tune chemical composition, size, shape, surface morphology, and internal structure of microgels by bringing in contact multiple fluid streams in a highly controlled fashion using versatile channel geometries and flow configurations and allowing controlled crosslinking.
Testing Quantum Effects of Gravity and Dark Energy at Laboratory Scales
Authors: Sivaram C , Kenath Arun , Prasad Avijeet
Published: May 2021

One of the biggest challenges in modern physics is how to unify gravity with quantum theory. There is an absence of a complete quantum theory of gravity, and conventionally it is thought that the effects of quantum gravity occur only at high energies (Planck scale). Here we suggest that certain novel quantum effects of gravity can become significant even at lower energies and could be tested at laboratory scales. We also suggest a few indirect effects of dark energy that can show up at laboratory scales. Using these ideas, we set observational constraints on radio recombination lines of the Rydberg atoms. We further suggest that high-precision measurements of Casimir effects for smaller plate separation could also show some manifestations of the presence of dark energy.
Energy in buildings: A review of models on hygrothermal transfer through the porous materials for building envelop
Authors: Ndukwu Macmanus Chinenye , Simo-Tagne Merlin , Ekop Ifiok Edem , Ibeh Mathew I. , Allen Maureen A. , Abam Fidelis. I. , Kharchi Razika
Published: May 2021

The hygrothermal transfer is very important for the design of a building envelope for thermal comfort and economic and energy analysis of the building envelope. The applications of various materials in building envelope have been studied extensively. The study presents several models for the hygrothermal transfer for various building walls. Several energy and mass conservation equations with different boundary conditions and input considerations were presented in this paper for concrete, bricks and wooden walls. The effect of hysteresis was ignored in developing most model equations, while few considered flow pattern of fluid through the wall surfaces. Due to the flexibility of Luikov models, it formed the basis for modelling the coupled heat and mass transfer for porous material independent of hygroscopic nature with different boundary conditions defined according to the geometry and orientations. The influence of type of wall, orientation, thickness, the density of the material and climatic variations on the temperature and moisture evolutions within the building materials was more pronounced. Literature, presenting imaging models using imagery software like COMSOL multi-physics, CFD etc. were scarce considering that microscopic imagery is now deployed to measure the heat and moisture evolution in materials. Future models should include shrinkage or expansion influence on the fibrous material like wood due to their behaviour under environmental condition.
Acute Lymphoblastic Leukemia Detection from Microscopic Images Using Weighted Ensemble of Convolutional Neural Networks
Authors: Mondal Chayan , Hasan Md. Kamrul , Jawad Md. Tasnim , Dutta Aishwariya , Islam Md. Rabiul , Awal Md. Abdul , Ahmad Mohiuddin , Alyami Salem A. , Ali Moni Mohammad
Published: May 2021

Although automated Acute Lymphoblastic Leukemia (ALL) detection is essential, it is challenging due to the morphological correlation between malignant and normal cells. The traditional ALL classification strategy is arduous, time-consuming, often suffers inter-observer variations, and necessitates experienced pathologists. This article has automated the ALL detection task, employing deep Convolutional Neural Networks (CNNs). We explore the weighted ensemble of deep CNNs to recommend a better ALL cell classifier. The weights are estimated from ensemble candidates' corresponding metrics, such as accuracy, F1-score, AUC, and kappa values. Various data augmentations and pre-processing are incorporated for achieving a better generalization of the network. We train and evaluate the proposed model utilizing the publicly available C-NMC-2019 ALL dataset. Our proposed weighted ensemble model has outputted a weighted F1-score of 88.6%, a balanced accuracy of 86.2%, and an AUC of 0.941 in the preliminary test set. The qualitative results displaying the gradient class activation maps confirm that the introduced model has a concentrated learned region. In contrast, the ensemble candidate models, such as Xception, VGG-16, DenseNet-121, MobileNet, and InceptionResNet-V2, separately produce coarse and scatter learned areas for most example cases. Since the proposed ensemble yields a better result for the aimed task, it can experiment in other domains of medical diagnostic applications.
Cardiac Myofibroblast and Fibrosis
Authors: Kurose Hitoshi
Published: May 2021

Fibroblasts are differentiated to myofibroblasts and produce collagen and other extracellular matrix when the heart is exposed to stresses. Myocardial infarction and pressure overload-induced hypertrophy are major stresses to induce differentiation of fibroblasts. Since collagen can compensate the missing tissue due to injury, appropriate production of collagen is beneficial for the injured heart against rupture. However, excessive deposition of collagen is called fibrosis and causes cardiac dysfunction. After fibroblasts are differentiated to myofibroblasts, myofibroblasts can further change their phenotypes. In addition, myofibroblasts are found to have a new function other than collagen production. Myofibroblasts have macrophage-like functions that engulf dead cells and secrete anti-inflammatory cytokines. So far, research on fibroblasts has been delayed due to the lack of available markers for selective isolation of fibroblasts. In recent years, it has become possible to genetically label fibroblasts, sequence the cells at single cell levels, and manipulate function or the number of cells. Based on new technologies, the origin of fibroblasts and myofibroblasts, time-dependent changes of fibroblast states after injury, and heterogeneity have been demonstrated. Here, I will introduce recent advances in fibroblasts and myofibroblasts.
Assessing Loss and Damage of Low Exposed Sudden Onset Disasters: Evidence from the Marginal Salt Cultivators of KutubdiaIsland, Bangladesh
Authors: Rahman Mohammad Mahbubur , Bijoy Mizanur Rahman
Published: May 2021

In recent years in Bangladesh, there has been regular cyclonic event, flooding and erratic pre-monsoons precipitation that has hampered production greatly and forced Bangladesh to import salt from abroad to manage market deficiency. There is much uncertainty about the effects of climate change on the frequency and intensity of small-scale, sudden onset weather phenomena such as heavy rainfall and subsequent loss and damage (L&D). But, several studies indicate that an obvious strong relationship exists between irregular rainfall and associated L&D. Nowadays, severe changing rainfall patterns are observed in Bangladesh, which is rapid-onset in nature, but low exposed in terms of response. The current study explored a ‘double-exposed’ burden combined of both climatic (e.g., uneven rainfall) and non-climatic governance factors (e.g., imperfect trade policy, the absence of risk transfer mechanisms) which are hindering salt production and pushing the country from the aspiration of salt exporting to the net buyer. This chapter mainly assesses the impacts of L&D due to climatic events that are causing overwhelming effects on the well-being of marginal salt farmers at Kutubdia Upazila of Bangladesh. Data were mainly collected through Participatory Vulnerability Analysis (PVA), Key informant interviews (KII), and Sample Surveys (SS). This study would provide insights for improved disaster management policy and an appropriate adaptive measure to address such extreme events as well as to initiate a further study for understanding the nexus of ‘nature and market’ in building resilience among the marginal salt farmers.
Productivity and Cost Models for the First Commercial Thinning of a Scots Pine Stand Using an Excavator with an Arbro 400S Harvester Head and a Farm Tractor Coupled to a Logging Trailer
Authors: Leszczyński Krzysztof , Stańczykiewicz Arkadiusz , Kulak Dariusz , Szewczyk Grzegorz , Tylek and Paweł
Published: May 2021

The objective of the present work was to determine the productivity and costs of timber harvesting and skidding during the first commercial thinning of a Scots pine stand. The analyzed harvesting set consisted of a mini-excavator (34 kW) with an stroke harvester head (gripping range: 4–30 cm), and a farm tractor coupled to an logging trailer with a hydraulic crane. Merchantable timber (roundwood with a minimum diameter of 5 cm inside bark) was harvested from a 25-year old planted Scots pine stand growing on a grid of 1.4×1.8 m. The study showed the productivity of the mini-harvester range from 3.09 to 3.47 m3∙PMH15 -1, and that of the forwarding set to be 4.07 m3∙PMH15 -1. The analyzed model of productivity as a function of individual tree volume and thinning intensity was statistically significant, but the intensity parameter was significant only on plots located along wide access trails (3.7 m) and insignificant on plots located along the narrow access trial (2.5m). The intertree distance was not found to be significant. The calculated net machine costs for the forwarding set and mini-harvester were 36.12 Euro∙PMH 1 and 52.47 Euro∙PMH-1, respectively. An increase in the utilization rate of the harvesting set to 80% would reduce the timber harvesting and skidding costs to 22.07 Euro∙m-3.
The Topological Universe’s Topological Materials
Authors: Bartlett Rodney
Published: May 2021

The part of this article dealing with topological insulators and topological superconductors was first written about two years ago - the ideas in the part about the topological universe originated six years ago or more. It’s rather strange that I never put the two parts together in writing before. My belief in unification is unshakeable - I’ve been convinced for years that the universe must be composed of topology. Since Earth is part of the cosmos, entanglement means it must have topological materials. The reverse is also true: topological materials on Earth are well known to science - so in a unification, space and time inevitably possess topological composition. Topological materials (topological insulators, topological superconductors) can be less mystifying if they’re related to the paradigm-shifting deterministic view of quantum mechanics which is described in the universal topology (the “rubber-sheet geometry” of the cosmos): see my previous submission “Hypothesis of Quantum Gravity - Resulting from a Static, Topological Universe Resulting from the Positives and Negatives of the Steady State and Big Bang Theories" at https://www.preprints.org/manuscript/202105.0239/v1 (the first section of this present article is a quick summary of the relevant parts).
Using Slit-Lamp Images for Deep Learning–Based Identification of Bacterial and Fungal Keratitis
Authors: Hung Ning , Kang Eugene Yu-Chuan , Shih Andy Guan-Yu , Lin Chi-Hung , Kuo Ming‐Tse , Hwang Yih-Shiou , Wu Wei-Chi , Kuo Chang-Fu , Hsiao Ching-Hsi
Published: May 2021

In this study, we aimed to develop a deep learning model for identifying bacterial keratitis (BK) and fungal keratitis (FK) by using slit-lamp images. We retrospectively collected slit-lamp images of patients with culture-proven microbial keratitis between January 1, 2010, and December 31, 2019, from two medical centers in Taiwan. We constructed a deep learning algorithm, consisting of a segmentation model for cropping cornea images and a classification model that applies convolutional neural networks to differentiate between FK and BK. The model performance was evaluated and presented as the area under the curve (AUC) of the receiver operating characteristic curves. A gradient-weighted class activation mapping technique was used to plot the heatmap of the model. By using 1330 images from 580 patients, the deep learning algorithm achieved an average diagnostic accuracy of 80.00%. The diagnostic accuracy for BK ranged from 79.59% to 95.91% and that for FK ranged from 26.31% to 63.15%. DenseNet169 showed the best model performance, with an AUC of 0.78 for both BK and FK. The heat maps revealed that the model was able to identify the corneal infiltrations. The model showed better diagnostic accuracy than the previously reported diagnostic performance of both general ophthalmologists and corneal specialists.
Renal and Red Marrow Dosimetry in Peptide Receptor Radionuclide Therapy: 20 Years of History and Ahead
Authors: Walrand Stephan , jamar francois
Published: May 2021

The development of dosimetry and studies in peptide receptor radionuclide therapy (PRRT) over these two last decades are reviewed. Differences in kidney and bone marrow toxicity reported between 90Y, 177Lu and external beam radiotherapy (EBRT) are discussed with regards to the physical properties of these beta emitter radionuclides. The impact of these properties on the response to small and large tumors is also considered. Capacities of the imaging modalities to assess the dosimetry to target tissues are evaluated. Studies published in the last two years that confirm a red marrow uptake in 177Lu-DOTATATE therapy, as already observed 20 years ago in 86Y-DOTATOC PET studies, are commented and analyzed in the light of the recent knowledges in transferrin transport mechanism. The review enlightens the importance i) of using state of the art imaging modalities, ii) of individualizing the activity to be injected with regards to the huge tissue uptake variability observed between patients, iii) of challenging the currently used but inappropriate blood based red marrow dosimetry and iv) of considering individually tandem therapy. Last, a smart individually optimized tandem therapy taking benefit of the bi-orthogonal toxicity-response pattern of 177Lu-DOTATATE and of 90Y-DOTATOC is proposed.
Interspecies Behavioral Variability of Medaka Fish Assessed by Comparative Phenomics
Authors: Audira Gilbert , Siregar Petrus , Chen Kelvin H.-C. , Roldan Marri Jmelou M. , Huang Jong-Chin , Lai Hong-Thih , Hsiao Chung-Der
Published: May 2021

Recently, medaka has been used as a model organism in various research fields. However, even though it possesses several advantages over zebrafish, fewer studies were done in medaka than zebrafish, especially its behaviour. Thus, to provide more information regarding its behaviour and to demonstrate the be-havioural differences between several species of medaka, we compared the behavioural performance and biomarker expression in the brain between four medaka fishes, which were Oryzias latipes, O. dancena, O. woworae, and O. sinensis. From the results, we found that each medaka species explicitly exhib-ited different behaviours to each other, which might be related to the different basal levels of several biomarkers. Furthermore, by phenomics and ge-nomic-based clustering, the differences between these medaka fishes were further investigated. Interestingly, even though both phenomics and ge-nomic-based clustering showed some resemblances to each other in terms of the interspecies relationship between medaka and zebrafish, however, in the medaka interspecies comparisons, this similarity was not displayed. Therefore, these results suggest a re-interpretation of several prior studies in comparative biology. We hope that these results contribute to the growing database of medaka fish phenotypes and provide one of the foundations for future phe-nomics studies of medaka fish.
Explainable Hopfield Neural Networks by Using an Automatic Video Generation System
Authors: Manzano Clemente Rubio
Published: May 2021

Hopfield Neural Networks (HNNs) are recurrent neural networks used to implement associative memory. Their main feature is their ability to pattern recognition, optimization, or image segmentation. However, sometimes it is not easy to provide the users with good explanations about the results obtained with them due to mainly the large number of changes in the state of neurons (and their weights) produced during a problem of machine learning. There are currently limited techniques to visualize, verbalize, or abstract HNNs. This paper outlines how we can construct automatic video generation systems to explain their execution. This work constitutes a novel approach to get explainable artificial intelligence systems in general and HNNs in particular building on the theory of data-to-text systems and software visualization approaches. We present a complete methodology to build these kinds of systems. Software architecture is also designed, implemented, and tested. Technical details about the implementation are also detailed and explained. Finally, we apply our approach for creating a complete explainer video about the execution of HNNs on a small recognition problem.
Effect of Blend Oils from Waste Paring Coconut and Candlenut and Selenium for Formulating of Feed-in Nile Tilapia and Production Parameters
Authors: Haetami Kiki , Junianto Junianto , Iskandar Iskandar , Abun Abun
Published: May 2021

This study aims to get growth and feed efficiency of Nile tilapia (Oreochromis niloticus) with plant oils supplement from paring coconut (P.C.) and candlenut (C.N.) enrichment of Se in rations formula. The oil of P.C. was dominated by saturated fatty acid (SFA) lauric (42.67%), while the extract of C.N. was unsaturated fatty acids (UFA), linoleic (34.4%), and oleic (48.99%). The extract of P.C. and C.N. or mix oils added 4% in basal ration formula (28% crude protein (C.P.) with energy-protein ratio 8 kcal/kg). Completely Randomized Design (6 × 3) consists R1: basal ration; R2: basal formula with blend of paring coconut and candlenut oils (2% PC + 2% CN); R3: blend oils (R2) with trace additive Se; R4: 4% PC + Se; R5: 4% CN + Se; R6: control ration (32% CP). The result of production parameters showed that blend oils supplement enrichment Se 0.15 ppm in feed formula with ratio SFA: UFA = 1: 1 was the best growth rate equal with high protein feed. Feed efficiency ranged from 50.14-57.93% and protein efficiency ratio 1.72-2.06 both for CN oil (SFA: UFA = 1 : 2), paring coconut (SFA : UFA = 2 : 1) or blend oils (SFA : UFA = 1 : 1). Incorporation of blend oils with Se can be used for Nile tilapia fingerlings (≥ 10 g). Paring coconut oil was trend increasing on feed efficiency for tilapia bigger stadium (≥ 30 g).
Coat colour inheritance in American mink (Neovison vison): Pedigree analysis
Authors:   Wrocław University of Environmental and Life Sciences, Department of Genetics, extsuperscript{ }Kożuchowska 7, 51-631 Wrocław, Poland, Wacławik Patrycja , Grabolus Dominika ,   Wrocław University of Environmental and Life Sciences, Department of Genetics, extsuperscript{ }Kożuchowska 7, 51-631 Wrocław, Poland, Zatoń-Dobrowolska Magdalena ,   Wrocław University of Environmental and Life Sciences, Department of Genetics, extsuperscript{ }Kożuchowska 7, 51-631 Wrocław, Poland, Kruszyński Wojciech ,   Wrocław University of Environmental and Life Sciences, Department of Genetics, extsuperscript{ }Kożuchowska 7, 51-631 Wrocław, Poland
Published: May 2021

This work aims to establish a simplified genotype for American mink (Neovison vison), on the basis of a group of basic genes (Asip, Tyrp–1, Tyr, Myo–5a, and Mc–1r) and three modifying genes (fawn, Ednrb, and Kit). The analysis used pedigrees of 61 females of standard brown, palomino, and silverblue colour variations. The database covered 380 offspring in nine colour variations: brown, silverblue, palomino, brown cross, palomino cross, pearl, pastel, silverpastel, and white. The analysis led to a simplified genotype explaining the principles of inheritance of most common coat colour variations in Polish mink farms. Due to the limited number of animals and the limited number of colour variations used, the analysis could not test the inheritance of all colours found in mink. The genotype was constructed on the basis of the homologous genes responsible for coat colour found in most animal species.
Analysis of mineral compounds in dry dog foods and their compliance with nutritional guidelines
Authors:   Department of Monogastric Animal Sciences, Laboratory of Animal Feed and Food, West Pomeranian University of Technology in Szczecin, Klemensa Janickiego 29, 71-270 Szczecin, Poland, Kępińska-Pacelik Jagoda , Biel Wioletta ,   Department of Monogastric Animal Sciences, Laboratory of Animal Feed and Food, West Pomeranian University of Technology in Szczecin, Klemensa Janickiego 29, 71-270 Szczecin, Poland
Published: May 2021

The aim of this study was to estimate complete extruded dry food for adult dogs, with a particular focus on: mineral profiles and ratios and the division into breed size. Dog foods were subjected to chemical analyzes to determine the content of macroelements and microelements. Additionally, the presence of heavy metals was verified and mineral ratios were estimated. The material for the research consisted of 15 randomly selected industrial household maintenance foods for adult dogs (five for large breed dogs, five for small breed dogs and five for all breeds dogs). All analyzed feeds met the FEDIAF recommended minimum recommended levels of microelements and macroelements, although the potassium level in 33% of the feeds analyzed was below the recommended minimum. 20% of the feeds tested exceeded the permissible quantitative ratio of calcium to phosphorus. In none of the tested feeds no heavy metals (Ni, Cd, Pb) were found. When choosing a feed, always pay attention to the label and carefully analyze the component of the product.
Gastrointestinal helminths and coccidia infestation of city pigeons (Columba livia forma urbana) on selected monuments in Szczecin
Authors:   Department of Biotechnology of Animal Reproduction and Environment Hygiene, West Pomerania University of Technology, Klemensa Janickiego 32, 71-270 Szczecin, Poland, Balicka-Ramisz Aleksandra , Laurans Łukasz ,   Clinic of Infectious Diseases, Hepatology and Liver Transplantation, Pomeranian Medical University, Arkońska 4, 71-455 Szczecin, Poland, Ramisz Anna ,   Student, Pomeranian Medical University, Szczecin, Poland, Stolbowa Elena ,   State Russian Museum, Inzhenernaya 4, Sankt-Petersburg, Russian Federation, Bal Wojciech ,   Department of Contemporary Architecture, Design Theory and Methodology, West Pomeranian University of Technology in Szczecin, Żołnierska 50, 71-210 Szczecin Poland
Published: May 2021

City pigeons (Columba livia forma urbana) are an integral part of the urbanized environment. However, they host parasites that can be passed zoonotically to humans and to domestic and wild birds. The aim of the study was to establish the current prevalence of nematode and coccidians infestation among city pigeons roosting in old buildings in Szczecin. The pigeon kits, comprising city pigeons and stray breeding pigeons which had not returned to their lofts, numbered from 45 to 60 individuals. Samples of pigeon droppings were tested qualitatively by the flotation method and then quantitatively by the McMaster technique. Oocyst culture was also performed in a humid chamber at a temperature of 24–26° C. The oocytes were identified using standard keys. Two roundworm families were identified, Ascaridia spp. (two species) and Capillaria spp., and three coccidian species: Eimeria labbeana, E.columbarum and E. columbae. Overall, the extent of infection was approximately 100% throughout the study area, but varied depending on the place of origin of the material. The prevalence of worms with eggs ranged from 9% to 16% for Ascaris spp. and 24–41% for Capillaria spp. The maximum OPG values, i.e. the number of oocytes per gram of faeces, were 360 for Ascaris spp. and 1134 for Capillaria spp; these indicate the intensity of infection. The most frequently observed species was Eimeria labbeana, whose prevalence was found to range from 82% to 96% depending on location, with a mean infection intensity of 1130–1870 OPG. The prevalence of the other two Eimeria species was also high, with respectively values of 56–77% for E. columbarum and 32–43% for E. columbae, depending on the location. The mean OPG values for the species were 430–1770 and 29–52, respectively. Maximum OPG values were 223,000 for E. labbeana, 54,000 for E. columbarum and 830 for E. columbae.
Analysis items of the four-tier immune system multiple choice test instrument using rasch model
Authors: Andriani Feni , Indrowati Meti , Sugiharto Bowo
Published: May 2021

The purpose of this study was to analyze the feasibility of the items of the four-tier multiple-choice test immune system instrument that had been developed. The development of the instrument using the Treagust (1988) model, namely defining content, collecting student misconceptions information, and developing a diagnostic test. A total of 25 items have been developed. The results of the instrument development were tested on 142 students of grade XI from several high schools in Surakarta who were selected by simple random sampling. The data analysis technique was performed using Rasch analysis in the Winstep application. The results of the construct validity test showed items number 5, 7, and 9 did not fit the validity standards. The reliability test shows that the value of Cronbach Alpha reliability is bad (n = 0.51), the value of the reliability item is special (no = 0.97), the value of person reliability is sufficient (n = 0.68), the value of person separation is weak (n = 1.44), and the item separation value is special (n = 5.38). The person discrimination test showed student 056P31 has the highest ability and student 098P51 has the lowest ability. The item discrimination test shows item number 1 is the best item and the bad item is number 14. The item difficulty analysis showed less proportionality because there were too many items in the easy and difficult categories. An expansion of the sample is needed to see a more comprehensive and diverse range of responses to instruments.
Youth‒Police Contact: Burdens and Inequities in an Adverse Childhood Experience, 2014‒2017
Authors: Geller Amanda
Published: May 2021

Objectives. To assess police contact as a potential adverse childhood experience by measuring its prevalence, nature, and distribution among urban adolescents.

Methods. Detailed US population-based data on youth‒police contact were collected in the Fragile Families and Child Wellbeing Study (n = 2478) from 2014 to 2017. Using regression modeling, I assessed adolescents’ police exposure and the magnitude and robustness of racial disparities in police contact. Sensitivity analyses examined disparities by behavior and socioeconomic context.

Results. Urban youths are heavily policed, beginning in preadolescence. Exposure to policing is unevenly distributed, with non-White adolescents—particularly Black boys—reporting more, and more aggressive, contact than their White counterparts. Hispanic‒White differences and disparities in girls’ experiences were less pronounced but present, particularly in how intrusive stops were. Intrusion disparities were robust to most behavioral controls, but not observed among youths with higher socioeconomic status.

Conclusions. Given extant literature documenting adverse health consequences of police encounters, findings implicate policing as a driver of health disparities in adolescence and throughout the life course. Public health infrastructure dedicated to the prevention and treatment of adverse childhood experiences is well suited for mitigating these harms and inequities.
Factors Associated With Calling 911 for an Overdose: An Ethnographic Decision Tree Modeling Approach
Authors: Wagner Karla D. , Koch Brandon , Bowles Jeanette M. , Verdugo Silvia R. , Harding Robert W. , Davidson Peter J.
Published: May 2021

Objectives. To identify factors that influence when people who use drugs (PWUDs) call 911 for an overdose.

Methods. We conducted 45 qualitative interviews and 180 surveys with PWUDs who had recently witnessed overdoses in Southern California from 2017 to 2019. We used conditional inference tree and random forest models to generate and validate a model to predict whether 911 would be called.

Results. Our model had good in- (83%) and out-of-sample (84%) predictive accuracy. Three aspects of the social and policy environment influenced calling 911 for an overdose: the effectiveness of response strategies employed, the behavior of other bystanders, and whether the responder believes it is their responsibility to call.

Conclusions. Even in the presence of policies that provide some protections, PWUDs are faced with difficult decisions about calling 911 and must weigh their own safety against that of an overdose victim. Potential interventions include strengthening training and safety planning for PWUDs, bolstering protections for PWUDs when they call 911, and separating law enforcement response from emergency medical response to overdoses.
Digital Health Engagement in the US Population: Insights From the 2018 Health Information National Trends Survey
Authors: Ratcliff Chelsea L. , Krakow Melinda , Greenberg-Worisek Alexandra , Hesse Bradford W.
Published: May 2021

Objectives. To examine prevalence and predictors of digital health engagement among the US population.

Methods. We analyzed nationally representative cross-sectional data on 7 digital health engagement behaviors, as well as demographic and socioeconomic predictors, from the Health Information National Trends Survey (HINTS 5, cycle 2, collected in 2018; n = 2698–3504). We fitted multivariable logistic regression models using weighted survey responses to generate population estimates.

Results. Digitally seeking health information (70.14%) was relatively common, whereas using health apps (39.53%) and using a digital device to track health metrics (35.37%) or health goal progress (38.99%) were less common. Digitally communicating with one’s health care providers (35.58%) was moderate, whereas sharing health data with providers (17.20%) and sharing health information on social media (14.02%) were uncommon. Being female, younger than 65 years, a college graduate, and a smart device owner positively predicted several digital health engagement behaviors (odds ratio range = 0.09–4.21; P value range < .001–.03).

Conclusions. Many public health goals depend on a digitally engaged populace. These data highlight potential barriers to 7 key digital engagement behaviors that could be targeted for intervention.
Minimal Clinically Important Differences for Patient-Reported Outcomes After TKA Depend on Central Sensitization
Authors: Kim Man Soo , Koh In Jun , Choi Keun Young , Seo Jeong Yong , In Yong
Published: May 2021

Background:

To our knowledge, it is still unknown if central sensitization (CS) influences the magnitude of the minimal clinically important difference (MCID) for patient-reported outcome measures after total knee arthroplasty (TKA). The purpose of this study was to determine the influence of CS on the MCID for the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) score in patients who underwent TKA for knee osteoarthritis.

Methods:

A total of 422 patients who underwent unilateral TKA and completed a 2-year follow-up were enrolled in this study. CS was measured using the Central Sensitization Inventory (CSI). The WOMAC score was used to evaluate preoperative and postoperative patient-reported outcomes. The measurement of the MCID was performed separately for patients with and without CS using both the anchor-based method and the distribution method. The change difference method defined the MCID as the difference in preoperative-to-postoperative change between the minimal-improvement group and the no-change group. In addition, the MCID was calculated using receiver operating characteristic (ROC) curve analysis. The percentage of MCID achievement in each group was also compared.

Results:

According to the change difference method, the MCID for the WOMAC total score was 23.4 points for patients with CS and 14.7 points for patients without CS. The MCID using the ROC cutoff value for the WOMAC total score was 29.5 points for the patients with CS and 26.5 points for the patients without CS. MCID achievement rates in WOMAC pain, function, and total scores were all found to be significantly higher in the patients without CS through the change difference method and the ROC method (all p < 0.05).

Conclusions:

The MCID for the WOMAC score of patients with CS after TKA was greater than that for patients without CS. Furthermore, by applying the calculated MCID to the group to which the patients belonged (with or without CS), we determined that patients with CS showed a lower MCID achievement rate than patients without CS.

Level of Evidence:

Prognostic Level III. See Instructions for Authors for a complete description of levels of evidence.
Employment Negotiation Behaviours of Canadian Academic Librarians: An Exploratory Study
Authors: Cardozo Paula , Scott Emma
Published: May 2021

While a small number of studies have investigated salary negotiation in librarianship, none have focused on Canadian academic libraries. What are the motivations and barriers that impact employment-offer negotiation? When Canadian librarians negotiate, what subjects are discussed, and do they feel they were successful? This preliminary report is a descriptive summary on the results of a survey we conducted to explore the negotiation propensity and perceptions of Canadian academic librarians when presented with their most recent offer of employment. A better understanding of this topic may encourage negotiation conversations and empower librarians in future employment offer negotiations to improve salaries and working conditions.
The Emotional Labour of Public Library Work
Authors: Rodger Joanne , Erickson Norene
Published: May 2021

This study seeks to extend the research on the emotional labour of public library workers. Because emotional labour is a relatively new concept in library and information science research, researchers and practitioners need to better understand the emotional labour experiences of front-line workers in public libraries. A qualitative survey was distributed electronically to library workers in one Canadian province. Participants described meaningful experiences connecting with customers, but also identified major challenges in performing customer service work. Results showed that the public facing display of regulated emotions that is ingrained in library customer service training often conflicts with inner emotions. The inability to reconcile opposing emotions and perceived limited administrative support affects individual enjoyment of work and their personal well-being. Participants report exhaustion and burnout as outcomes of emotional labour. Library organizations must acknowledge the emotional labour aspect of library customer service work and provide more extensive formalized support for staff who are in customer service roles. Equipping staff with stronger emotional labour strategies might also help to build resilience and increase job satisfaction.
La peste negra bajomedieval (1348-1351 AD) en el valle del Tiétar (sierra de Gredos, Ávila): aspectos económicos y paleoambientales
Authors: Luelmo-Lautenschlaeger Reyes , Izdebski Adam , Blanco-González Antonio , Pérez-Díaz Sebastián , López-Sáez José Antonio
Published: May 2021

En el presente trabajo se evalúan los efectos de la peste negra bajomedieval (1348–1351 AD) sobre los bosques y las actividades agroforestales de un valle intramontano de la Meseta sur española, a partir de análisis polínicos de alta resolución. Para comprender mejor los efectos de aquella pandemia se analiza la secuencia paleoambiental de cinco turberas, a lo largo de un amplio recorrido cronológico entre los siglos XII y XIV. Este análisis demuestra que la peste provocó una desaceleración de las actividades agropecuarias –agricultura, arboricultura, ganadería– y la consiguiente recuperación de las masas forestales, salvo en las zonas de montaña donde un menor control de los pasos ganaderos incidió en cierta degradación del bosque.