Sintesis Hidroksiapatit secara Elektrolisis sebagai Bahan Baku Pembuatan Scaffold Tissue HA/PVA-AgCl untuk Aplikasi Penyembuhan Luka
Authors: Supriyono Supriyono , Poerwadi Bambang , Widhiyanuriyawan Denny , Wildah Nasirotul , Ardyarum Mahfita , Widadudari Nabila , Trizardi Ilhan Junio , Yustisia Ulya Ahda 
Published: October 2024

<p><span style="font-size: 8.5pt; font-family: 'Times New Roman', serif;">Hidroksiapatit (HA) sebagai bahan dasar dalam <em>scaffold tissue</em> memiliki kemampuan untuk berikatan langsung dengan jaringan tubuh secara <em>in vivo</em>. <em>Scaffold tissue</em> pada penelitian ini dalam bentuk membran komposit terdiri dari nHA/PVA-AgCl. AgCl berfungsi sebagai agen antibakteri, sedangkan PVA berfungsi sebagai polimer perekat. Ukuran nanometer untuk HA diperoleh melalui proses elektrolisis yang mengatur voltase untuk mengendalikan ukuran partikel. Variasi </span><span style="font-size: 8.5pt; font-family: 'Times New Roman', serif;" lang="PT-BR">potensial listrik</span><span style="font-size: 8.5pt; font-family: 'Times New Roman', serif;"> elektro</span><span style="font-size: 8.5pt; font-family: 'Times New Roman', serif;" lang="PT-BR">lisis</span><span style="font-size: 8.5pt; font-family: 'Times New Roman', serif;"> 4, 5, dan 6 V digunakan dalam proses selama </span><span style="font-size: 8.5pt; font-family: 'Times New Roman', serif;" lang="PT-BR">enam</span><span style="font-size: 8.5pt; font-family: 'Times New Roman', serif;"> jam. Sebagai pembanding, HA komersil juga digunakan dalam penelitian ini dan hasilnya menunjukkan bahwa voltase 5 V menghasilkan HA yang murni tanpa adanya <em>brushite</em>. Dalam hal diameter partikel, HA yang disintesis dengan potensial listrik 4, 5, dan 6 V, serta HA komersil masing-masing memiliki diameter 232 nm, 276 nm, 355 nm, dan 5421 nm. Metode BET untuk pengujian luas permukaan menghasilkan nilai berturut-turut untuk variabel potensial listrik elektrolisis HA 4, 5, dan 6 V, serta HA komersil masing-masing adalah 317,22; 344,247; 323,419; dan 205,779 m<sup>2</sup>/g. Hasil uji pre-klinis selama tujuh hari menunjukkan bahwa <em>scaffold tissue</em> yang menggunakan HA sintesis dengan voltase 5 V menghasilkan pertumbuhan fibroblas yang paling baik, dengan nilai rata-rata sekitar 47,3±4,53 fibroblas/nm<sup>2</sup>.</span></p><p><strong><br /></strong></p><p><strong>Synthesis of Hydroxyapatite by Electrolysis as Raw Material for Making HA/PVA-AgCl Tissue Scaffold for Wound Healing Application</strong>. <strong><span style="font-size: 8.5pt; font-family: 'Times New Roman',serif; mso-fareast-font-family: Calibri; mso-fareast-theme-font: minor-latin; mso-ansi-language: IN; mso-fareast-language: EN-US; mso-bidi-language: AR-SA; mso-no-proof: yes;">. </span></strong><span style="font-size: 8.5pt; font-family: 'Times New Roman', serif;">Hydroxyapatite (HA), the primary material in scaffold tissue, can directly interact with the body's tissues in vivo. In this research, the scaffold tissue forms a composite membrane consisting of nHA/PVA-AgCl, with AgCl as an antibacterial agent and PVA as a binding polymer.</span><span style="font-size: 8.5pt; font-family: 'Times New Roman', serif;">Nanometer-sized HA particles are obtained through electro-synthesis, controlling voltage to regulate particle size. Three voltage variations, 4</span><span style="font-size: 8.5pt; font-family: 'Times New Roman', serif;" lang="EN-US">,</span><span style="font-size: 8.5pt; font-family: 'Times New Roman', serif;"> 5, and 6 V</span><span style="font-size: 8.5pt; font-family: 'Times New Roman', serif;">are used in the process for 6 hours. Commercial HA is also used for comparison, and the results indicate that 5 V voltage produces pure HA without brushite contamination. In terms of particle diameter, HA synthesized at 4, 5, 6 V, and commercial HA each have diameters of 232, 276, 355, and 5421 nm, respectively. The BET method is employed to test surface area, yielding consecutive values for the electro-synthesized HA voltage variations, with 4, 5, 6 V, and commercial HA having surface areas of 317.22</span><span style="font-size: 8.5pt; font-family: 'Times New Roman', serif;" lang="EN-US">;</span><span style="font-size: 8.5pt; font-family: 'Times New Roman', serif;"> 344.247</span><span style="font-size: 8.5pt; font-family: 'Times New Roman', serif;" lang="EN-US">; </span><span style="font-size: 8.5pt; font-family: 'Times New Roman', serif;">323.419</span><span style="font-size: 8.5pt; font-family: 'Times New Roman', serif;" lang="EN-US">; </span><span style="font-size: 8.5pt; font-family: 'Times New Roman', serif;">and 205.779 m<sup>2</sup>/g, respectively. The pre-clinical test in 7-day</span><span style="font-size: 8.5pt; font-family: 'Times New Roman', serif;" lang="EN-US">s</span><span style="font-size: 8.5pt; font-family: 'Times New Roman', serif;"> period findings reveal that scaffold tissue using HA synthesized at 5</span><span style="font-size: 8.5pt; font-family: 'Times New Roman', serif;">V voltage produces the best fibroblast growth, with an average value of approximately 47.3±4.53 fibroblasts</span><span style="font-size: 8.5pt; font-family: 'Times New Roman', serif;" lang="EN-US">/</span><span style="font-size: 8.5pt; font-family: 'Times New Roman', serif;">nm<sup>2</sup>.</span></p><div><div><div id="_com_2" class="msocomtxt"><!--[if !supportAnnotations]--></div><!--[endif]--></div></div>