bluuebunny/crossref_metadata_2025 huggingface dataset:
Thromboembolic Events with Cyclin-Dependent Kinase 4/6 Inhibitors in the FDA Adverse Event Reporting System
Authors: Raschi Emanuel , Fusaroli Michele , Ardizzoni Andrea , Poluzzi Elisabetta , De Ponti Fabrizio
Published: April 2021

We analyzed thromboembolic events, recognized (AESIs), with cyclin-dependent kinase (CDK)4/6 inhibitors, using the Food and Drug Administration adverse event reporting system. Methods: Thromboembolic events were characterized in terms of spectrum [venous and arterial thromboembolism (VTE; ATE)] and clinical features by combining the disproportionality approach [reporting odds ratio (ROR) with 95% confidence interval (CI)] with individual case assessment. Results: A total of 1722 thromboembolic events were retained. Increased VTE reporting emerged for CDK4/6 inhibitors in the exploratory analyses (n = 659; ROR = 1.51; 95% CI = 1.39–1.63), with consistent disproportionality in the consolidated analyses (e.g., deep vein thrombosis with abemaciclib: 17; 1.98; 1.22–3.19). Higher-than-expected ATE reporting was found for ribociclib, including myocardial infarction (41; 1.82; 1.33–2.48), with rapid onset (median latency 1 vs. 6 months for other CDK4/6 inhibitors). Causality was highly probable or probable in 83.2% of cases, with a negligible proportion of pre-existing drug- and patient-related risk factors except for cardiovascular comorbidities (26%). Conclusions: Although causal association cannot be firmly inferred, oncologists should proactively monitor the occurrence of VTE with CDK4/6 inhibitors. The unexpected distinctive increased ATE reporting with ribociclib deserves urgent clarification though large comparative population-based studies. We support pharmacovigilance for the post-marketing characterization of AESIs, thus promoting real-time safe prescribing in oncology.
Peripheral Blood from Rheumatoid Arthritis Patients Shows Decreased Treg CD25 Expression and Reduced Frequency of Effector Treg Subpopulation
Authors: Go Eunbyeol , Yoo Su-Jin , Choi Suyoung , Sun Pureum , Jung Min Kyung , Kwon Somin , Heo Bu Yeon , Kim Yeeun , Kang Ju-Gyeong , Kim Jinhyun , Shin Eui-Cheol , Kang Seong Wook , Kwon Jaeyul
Published: April 2021

Rheumatoid arthritis (RA) is a common autoimmune disease characterized by immune cell infiltration of the synovium, leading to the loss of cartilage, bone, and joint function. Although regulatory T (Treg) cells are thought to modulate the initiation and progression of RA, a consensus has yet to be reached regarding the function and composition of Treg cells in RA patients. To address these discrepancies, we analyzed not only the total Treg frequency but also that of Treg subpopulations in the peripheral blood of RA patients and healthy controls by flow cytometry. We found that the total Treg population was not significantly different between RA and control subjects. However, the effector Treg cell subgroup, defined as CD45RA−CD25hi, showed markedly decreased frequency in RA patients. In addition, the total Treg population from RA patients showed a significant decline in the expression of CD25. Both the naïve and effector Treg subgroups also showed marked reduction of CD25 expression in RA patients compared to controls. These data suggest that the decreased frequency of effector Treg cells and overall reduction of CD25 expression in Treg cells in the peripheral blood may be evidence of altered Treg homeostasis associated with RA pathogenesis.
Lysine 53 Acetylation of Cytochrome c in Prostate Cancer: Warburg Metabolism and Evasion of Apoptosis
Authors: Bazylianska Viktoriia , Kalpage Hasini A. , Wan Junmei , Vaishnav Asmita , Mahapatra Gargi , Turner Alice A. , Chowdhury Dipanwita Dutta , Kim Katherine , Morse Paul T. , Lee Icksoo , Brunzelle Joseph S. , Polin Lisa , Subedi Prabal , Heath Elisabeth I. , Podgorski Izabela , Marcus Katrin , Edwards Brian F.P. , Hüttemann Maik
Published: April 2021

Prostate cancer is the second leading cause of cancer-related death in men. Two classic cancer hallmarks are a metabolic switch from oxidative phosphorylation (OxPhos) to glycolysis, known as the Warburg effect, and resistance to cell death. Cytochrome c (Cytc) is at the intersection of both pathways, as it is essential for electron transport in mitochondrial respiration and a trigger of intrinsic apoptosis when released from the mitochondria. However, its functional role in cancer has never been studied. Our data show that Cytc is acetylated on lysine 53 in both androgen hormone-resistant and -sensitive human prostate cancer xenografts. To characterize the functional effects of K53 modification in vitro, K53 was mutated to acetylmimetic glutamine (K53Q), and to arginine (K53R) and isoleucine (K53I) as controls. Cytochrome c oxidase (COX) activity analyzed with purified Cytc variants showed reduced oxygen consumption with acetylmimetic Cytc compared to the non-acetylated Cytc (WT), supporting the Warburg effect. In contrast to WT, K53Q Cytc had significantly lower caspase-3 activity, suggesting that modification of Cytc K53 helps cancer cells evade apoptosis. Cardiolipin peroxidase activity, which is another proapoptotic function of the protein, was lower in acetylmimetic Cytc. Acetylmimetic Cytc also had a higher capacity to scavenge reactive oxygen species (ROS), another pro-survival feature. We discuss our experimental results in light of structural features of K53Q Cytc, which we crystallized at a resolution of 1.31 Å, together with molecular dynamics simulations. In conclusion, we propose that K53 acetylation of Cytc affects two hallmarks of cancer by regulating respiration and apoptosis in prostate cancer xenografts.
Bioprinting and Differentiation of Adipose-Derived Stromal Cell Spheroids for a 3D Breast Cancer-Adipose Tissue Model
Authors: Horder Hannes , Guaza Lasheras Mar , Grummel Nadine , Nadernezhad Ali , Herbig Johannes , Ergün Süleyman , Teßmar Jörg , Groll Jürgen , Fabry Ben , Bauer-Kreisel Petra , Blunk Torsten
Published: April 2021

Biofabrication, including printing technologies, has emerged as a powerful approach to the design of disease models, such as in cancer research. In breast cancer, adipose tissue has been acknowledged as an important part of the tumor microenvironment favoring tumor progression. Therefore, in this study, a 3D-printed breast cancer model for facilitating investigations into cancer cell-adipocyte interaction was developed. First, we focused on the printability of human adipose-derived stromal cell (ASC) spheroids in an extrusion-based bioprinting setup and the adipogenic differentiation within printed spheroids into adipose microtissues. The printing process was optimized in terms of spheroid viability and homogeneous spheroid distribution in a hyaluronic acid-based bioink. Adipogenic differentiation after printing was demonstrated by lipid accumulation, expression of adipogenic marker genes, and an adipogenic ECM profile. Subsequently, a breast cancer cell (MDA-MB-231) compartment was printed onto the adipose tissue constructs. After nine days of co-culture, we observed a cancer cell-induced reduction of the lipid content and a remodeling of the ECM within the adipose tissues, with increased fibronectin, collagen I and collagen VI expression. Together, our data demonstrate that 3D-printed breast cancer-adipose tissue models can recapitulate important aspects of the complex cell–cell and cell–matrix interplay within the tumor-stroma microenvironment.
Genotoxic Bystander Signals from Irradiated Human Mesenchymal Stromal Cells Mainly Localize in the 10–100 kDa Fraction of Conditioned Medium
Authors: Kohl Vanessa , Fabarius Alice , Drews Oliver , Bierbaum Miriam , Jawhar Ahmed , Darwich Ali , Weiss Christel , Flach Johanna , Brendel Susanne , Kleiner Helga , Seifarth Wolfgang , Hofmann Wolf-Karsten , Popp Henning D.
Published: April 2021

Genotoxic bystander signals released from irradiated human mesenchymal stromal cells (MSC) may induce radiation-induced bystander effects (RIBEs) in human hematopoietic stem and progenitor cells (HSPC), potentially causing leukemic transformation. Although the source of bystander signals is evident, the identification and characterization of these signals is challenging. Here, RIBEs were analyzed in human CD34+ cells cultured in distinct molecular size fractions of medium, conditioned by 2 Gy irradiated human MSC. Specifically, γH2AX foci (as a marker of DNA double-strand breaks) and chromosomal instability were evaluated in CD34+ cells grown in approximate (I) < 10 kDa, (II) 10–100 kDa and (III) > 100 kDa fractions of MSC conditioned medium and un-/fractionated control medium, respectively. Hitherto, significantly increased numbers of γH2AX foci (p = 0.0286) and aberrant metaphases (p = 0.0022) were detected in CD34+ cells grown in the (II) 10–100 kDa fraction (0.67 ± 0.10 γH2AX foci per CD34+ cell ∨ 3.8 ± 0.3 aberrant metaphases per CD34+ cell sample; mean ± SEM) when compared to (I) < 10 kDa (0.19 ± 0.01 ∨ 0.3 ± 0.2) or (III) > 100 kDa fractions (0.23 ± 0.04 ∨ 0.4 ± 0.4) or un-/fractionated control medium (0.12 ± 0.01 ∨ 0.1 ± 0.1). Furthermore, RIBEs disappeared after heat inactivation of medium at 75 °C. Taken together, our data suggest that RIBEs are mainly mediated by the heat-sensitive (II) 10–100 kDa fraction of MSC conditioned medium. We postulate proteins as RIBE mediators and in-depth proteome analyses to identify key bystander signals, which define targets for the development of next-generation anti-leukemic drugs.
Australian Foster Carers’ Views and Concerns Regarding Maternal Drug Use and the Safety of Breastmilk
Authors: Blythe Stacy , Peters Kath , Elcombe Emma , Burns Elaine , Gribble Karleen
Published: April 2021

Parental substance misuse and mental health issues are major factors associated with infant placement into out-of-home care. Such placements may result in disruption and/or cessation of breastfeeding. Provision of breastmilk to infants in out-of-home care (OOHC) is desirable in terms of infant health and development, and also in supporting maternal caregiving. However, little is known about how breastfeeding is supported for infants in out-of-home care. This study used an online survey to explore the facilitation of breastfeeding in the context of OOHC and foster carers’ management of expressed breastmilk (EBM). Foster carers were generally open to the idea of maternal breastfeeding and infants in their care receiving EBM from their mothers. However, the majority of respondents expressed concern regarding the safety of EBM for infant consumption due to the possibility of harmful substances in the milk. Concerns regarding the safety of handling EBM were also prevalent. These concerns caused foster carers to discard EBM. Findings suggest foster carers’ may lack knowledge related to maternal substance use and breastmilk. Better integration between health care and social service systems, where the voices of mothers, foster carers and child protection workers are heard, is necessary to develop solutions enabling infants living in OOHC access to their mother’s breastmilk.
Synthesis and Properties of p-Si/n-Cd1−xAgxO Heterostructure for Transparent Photodiode Devices
Authors: Anitha Mannarsamy , Deva Arun Kumar Karuppiah , Mele Paolo , Anitha Nagarajan , Saravanakumar Karunamoorthy , Sayed Mahmoud Ahmed , Ali Atif Mossad , Amalraj Lourdusamy
Published: April 2021

We developed silver-doped Cd1–xAgxO thin films (where x = 0, 0.01, 0.02, 0.03 and 0.04) on amorphous glass substrate by an automated nebulizer spray pyrolysis set-up. The XRD patterns show rock salt cubic crystal structures, and the crystallite sizes vary with respect to Ag doping concentrations. SEM images exhibited a uniform distribution of grains with the addition of Ag; this feature could support the enhancement of electron mobility. The transmittance spectra reveal that all films show high transmittance in the visible region with the observed bandgap of about 2.40 eV. The room temperature photoluminescence (PL) studies show the increase of near-band-edge (NBE) emission of the films prepared by different Ag doping levels, resulting in respective decreases in the bandgaps. The photodiode performance was analyzed for the fabricated p-Si/n-Cd1–xAgxO devices. The responsivity, external quantum efficiency and detectivity of the prepared p-Si/n-Cd1–xAgxO device were investigated. The repeatability of the optimum (3 at.% Ag) photodiode was also studied. The present investigation suggests that Cd1–xAgxO thin films are the potential candidates for various industrial and photodetector applications.
A Comprehensive Review on the Performance of Structural Lightweight Aggregate Concrete for Sustainable Construction
Authors: Agrawal Yash , Gupta Trilok , Sharma Ravi , Panwar Narayan Lal , Siddique Salman
Published: April 2021

Lightweight aggregate concrete is an innovative building material used to reduce the self-weight of a high-rise building. Recently, the use of lightweight aggregate in construction is increasing immensely due to its performance during an earthquake. Lightweight aggregate concrete (LWAC) is a solution for the achievement of sustainability in the construction sector, which helps us cut down the overall cost of a project in massive construction work (tall buildings and bridges). Additionally, using various industrial by-products and waste instead of natural aggregate allows us to reduce the negative impact on the environment. The development of lightweight aggregate concrete with its relevance is still prominent. The performance of lightweight aggregate on various properties of concrete is explored in this study. This study shows that the lightweight aggregate and waste materials of less density can be used for structural applications with a strength equivalent to that of normal weight concrete. The application and advantages of LWAC are also discussed in this study. The paper’s overall finding reveals that LWAC can be used in sustainable construction growth and reduce waste by using it as natural aggregate in concrete to maintain environmental sustainability.
Halogen Bonding Involving I2 and d8 Transition-Metal Pincer Complexes
Authors: Freindorf Marek , Yannacone Seth , Oliveira Vytor , Verma Niraj , Kraka Elfi
Published: April 2021

We systematically investigated iodine–metal and iodine–iodine bonding in van Koten’s pincer complex and 19 modifications changing substituents and/or the transition metal with a PBE0–D3(BJ)/aug–cc–pVTZ/PP(M,I) model chemistry. As a novel tool for the quantitative assessment of the iodine–metal and iodine–iodine bond strength in these complexes we used the local mode analysis, originally introduced by Konkoli and Cremer, complemented with NBO and Bader’s QTAIM analyses. Our study reveals the major electronic effects in the catalytic activity of the M–I–I non-classical three-center bond of the pincer complex, which is involved in the oxidative addition of molecular iodine I2 to the metal center. According to our investigations the charge transfer from the metal to the σ* antibonding orbital of the I–I bond changes the 3c–4e character of the M–I–I three-center bond, which leads to weakening of the iodine I–I bond and strengthening of the metal–iodine M–I bond, facilitating in this way the oxidative addition of I2 to the metal. The charge transfer can be systematically modified by substitution at different places of the pincer complex and by different transition metals, changing the strength of both the M–I and the I2 bonds. We also modeled for the original pincer complex how solvents with different polarity influence the 3c–4e character of the M–I–I bond. Our results provide new guidelines for the design of pincer complexes with specific iodine–metal bond strengths and introduce the local vibrational mode analysis as an efficient tool to assess the bond strength in complexes.
Old Donors for New Molecular Conductors: Combining TMTSF and BEDT-TTF with Anionic (TaF6)1−x/(PF6)x Alloys
Authors: Allain Magali , Mézière Cécile , Auban-Senzier Pascale , Avarvari Narcis
Published: April 2021

Tetramethyl-tetraselenafulvalene (TMTSF) and bis(ethylenedithio)-tetrathiafulvalene (BEDT-TTF) are flagship precursors in the field of molecular (super)conductors. The electrocrystallization of these donors in the presence of (n-Bu4N)TaF6 or mixtures of (n-Bu4N)TaF6 and (n-Bu4N)PF6 provided Bechgaard salts formulated as (TMTSF)2(TaF6)0.84(PF6)0.16, (TMTSF)2(TaF6)0.56(PF6)0.44, (TMTSF)2(TaF6)0.44(PF6)0.56 and (TMTSF)2(TaF6)0.12(PF6)0.88, together with the monoclinic and orthorhombic phases δm-(BEDT-TTF)2(TaF6)0.94(PF6)0.06 and δo-(BEDT-TTF)2(TaF6)0.43(PF6)0.57, respectively. The use of BEDT-TTF and a mixture of (n-Bu4N)TaF6/TaF5 afforded the 1:1 phase (BEDT-TTF)2(TaF6)2·CH2Cl2. The precise Ta/P ratio in the alloys has been determined by an accurate single crystal X-ray data analysis and was corroborated with solution 19F NMR measurements. In the previously unknown crystalline phase (BEDT-TTF)2(TaF6)2·CH2Cl2 the donors organize in dimers interacting laterally yet no organic-inorganic segregation is observed. Single crystal resistivity measurements on the TMTSF based materials show typical behavior of the Bechgaard phases with room temperature conductivity σ ≈ 100 S/cm and localization below 12 K indicative of a spin density wave transition. The orthorhombic phase δo-(BEDT-TTF)2(TaF6)0.43(PF6)0.57 is semiconducting with the room temperature conductivity estimated to be σ ≈ 0.16–0.5 S/cm while the compound (BEDT-TTF)2(TaF6)2·CH2Cl2 is also a semiconductor, yet with a much lower room temperature conductivity value of 0.001 to 0.0025 S/cm, in agreement with the +1 oxidation state and strong dimerization of the donors.
High Mobility Reactive Sputtered CuxO Thin Film for Highly Efficient and Stable Perovskite Solar Cells
Authors: Islam Mohammad Aminul , Wahab Yasmin Abdu , Khandaker Mayeen Uddin , Alsubaie Abdullah , Almalki Abdulraheem S. A. , Bradley David A. , Amin Nowshad
Published: April 2021

Copper oxide (CuxO) films are considered to be an attractive hole-transporting material (HTM) in the inverted planar heterojunction perovskite solar cells due to their unique optoelectronic properties, including intrinsic p-type conductivity, high mobility, low-thermal emittance, and energy band level matching with the perovskite (PS) material. In this study, the potential of reactive sputtered CuxO thin films with a thickness of around 100 nm has been extensively investigated as a promising HTM for effective and stable perovskite solar cells. The as-deposited and annealed films have been characterized by using X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Photoluminescence (PL), UV-Vis spectroscopy, and Hall-effect measurement techniques. The significant change in structural and optoelectronic properties has been observed as an impact of the thermal annealing process. The phase conversion from Cu2O to CuO, including grain size increment, was observed upon thermal annealing. The transmittance and optical bandgap were found to vary with the films’ crystallographic transformation. The predominant p-type conductivity and optimum annealing time for higher mobility have been confirmed from the Hall measurement. Films’ optoelectrical properties were implemented in the complete perovskite solar cell for numerical analysis. The simulation results show that a 40 min annealed CuxO film yields the highest efficiency of 22.56% with a maximum open-circuit voltage of 1.06 V.
Concordance between Response Assessment Using Prostate-Specific Membrane Antigen PET and Serum Prostate-Specific Antigen Levels after Systemic Treatment in Patients with Metastatic Castration Resistant Prostate Cancer: A Systematic Review and Meta-Analysis
Authors: Han Sangwon , Woo Sungmin , Kim Yong-il , Lee Jae-Lyun , Wibmer Andreas G. , Schoder Heiko , Ryu Jin-Sook , Vargas Hebert Alberto
Published: April 2021

Prostate-specific membrane antigen positron emission tomography (PSMA PET) has recently gained interest as a promising tool for treatment response evaluation in metastatic castration-resistant prostate cancer (CRPC). We performed a systematic review and meta-analysis assessing the concordance between response evaluation using PSMA PET and serum prostate-specific antigen (PSA) level after systemic treatment and the association between PSMA PET and overall survival in metastatic CRPC patients. PubMed, Embase, and Cochrane library databases were searched until August 2020. Studies that reported the concordance between PSMA PET and PSA response were included. PSMA PET and PSA response evaluation were dichotomized into response vs. non-response to construct two-by-two contingency tables; an ≥30% increase in PSMA PET according to PET Response Criteria in Solid Tumors 1.0 and as an increase in serum PSA level of ≥25% as per Prostate Cancer Working Group 3 guidelines were defined as non-response. The percent agreement rates were pooled using random-effect model. Ten studies (268 patients) were included. The concordance rates ranged 0.50–0.84 with a pooled proportion of 0.73 (95% confidence interval 0.67–0.79). Patients were treated with 177Lu-PSMA therapy in five, chemotherapy in three, 223Ra in one, and more than one type in one study. Various PET parameters were used: the most widely evaluated was PSMA tumor volume (PSMA-TV). Similar proportions were found across different therapeutic agents, PET response parameters, and regarding directionality of discordance (PSA response/PSMA non-response vs. PSMA response/PSA non-response). Two studies reported that a decrease in PSMA-TV was associated with better overall survival. PSMA PET and PSA response assessments were discordant in nearly a fourth of metastatic CRPC patients. Further studies are warranted to establish the clinical meaning of this discordance and define appropriate management for such clinical situation.
Change Point Test for the Conditional Mean of Time Series of Counts Based on Support Vector Regression
Authors: Lee Sangyeol , Lee Sangjo
Published: April 2021

This study considers support vector regression (SVR) and twin SVR (TSVR) for the time series of counts, wherein the hyper parameters are tuned using the particle swarm optimization (PSO) method. For prediction, we employ the framework of integer-valued generalized autoregressive conditional heteroskedasticity (INGARCH) models. As an application, we consider change point problems, using the cumulative sum (CUSUM) test based on the residuals obtained from the PSO-SVR and PSO-TSVR methods. We conduct Monte Carlo simulation experiments to illustrate the methods’ validity with various linear and nonlinear INGARCH models. Subsequently, a real data analysis, with the return times of extreme events constructed based on the daily log-returns of Goldman Sachs stock prices, is conducted to exhibit its scope of application.
Stable Isotope Analysis of Ozark Hellbender (Cryptobranchus alleganiensis bishopi) Living and Preserved Museum Tissue Reveals a Shift in Their Generalist Diet Composition
Authors: Hiler Waylon , Trauth Stanley E. , Wheeler Benjamin , Jimenez Aimee , Radanovic Milica , Milanovich Joseph R. , Christian Alan D.
Published: April 2021

Ozark hellbenders (Cryptobranchus alleganiensis bishopi) have undergone marked population declines across their entire distribution. A variety of ecological life history research has been conducted to determine the cause(s) of the declines. Historically, hellbender diet studies used stomach content examination methods; however, alternative approaches such as less intrusive stable isotope analyses are now options for researchers. The goals of our study were to conduct stable isotope analysis on live and formalin-preserved museum specimen Ozark hellbender tissues to identify diet composition in the Eleven Point and Spring rivers, Arkansas. Also, we used stable isotope analysis to investigate if Spring River hellbender diets have changed over time. We sampled fish, live hellbenders (non-destructively), and formalin-preserved hellbender tissues from museum collections for stable isotope analysis. We sampled crayfish for assemblage composition and stable isotope analysis. The results of our stable isotope study revealed three main findings: (1) there were no statistically significant differences between hellbender δ13C and δ15N values among sites and hellbender stable C and N isotopes were correlated with body length; (2) traditional δ13C versus δ15N bi-plots and trophic discrimination values did not provide complete discernment in hellbender diets; however, Bayesian MixSIAR models revealed hellbenders to be generalists, and (3) the use of δ13C and δ15N values adjusted historic formalin-fixed and ethanol preserved hellbenders matched well with current crayfish and fish stable isotope values based on Bayesian MixSIAR models. These findings provide important diet information and a possible tool to examine dietary patterns from preserved specimens that may be used for hellbender conservation and management.
Environment Management of Hydropower Development: A Case Study
Authors: Zhang Yakun , Tang Wenzhe , Duffield Colin F. , Zhang Lihai , Hui Felix Kin Peng
Published: April 2021

Environment management is one of the key aspects of hydropower development in acquiring sustainable energy. However, there has been limited research demonstrating the overall aspects of environment management of hydropower development with support of sound empirical evidence. In present study, the status of environment management in hydropower development was comprehensively investigated by conducting a case study based on the data collected from a field survey. The results show that, as environment management is largely subject to legal requirements, the environment management system needs to be established by integrating the legal requirements and needs of project implementation. This could potentially reduce the influence of legal restrictions on hydropower development. The main hydropower project environment management processes include identifying key environmental factors, implementation, monitoring, and performance measurement, which deal with environmental issues such as terrestrial and aquatic ecology protection, wastewater treatment, solid waste disposal, and acoustic-environment protection. Project participants should establish partnering relationships to cooperatively deal with environmental impacts of hydropower project development, in which public participation and sufficient resources input into environmental protection are essential for project success. The results of this study provide a sound basis for participants to deal with the key issues of environmental protection such as meeting legal requirements, training for improving environment management process, cost control, and cooperative environment management. The results of this study could help practitioners to tackle the interactions among project delivery, environmental protection, and engagement of local communities in an optimized way with the aim of maximizing effectiveness of the resources of all participants.
Scenario Analyses of Exhaust Emissions Reduction through the Introduction of Electric Vehicles into the City
Authors: Jacyna Marianna , Żochowska Renata , Sobota Aleksander , Wasiak Mariusz
Published: April 2021

In recent years, policymakers of urban agglomerations in various regions of the world have been striving to reduce environmental pollution from harmful exhaust and noise emissions. Restrictions on conventional vehicles entering the inner city are being introduced and the introduction of low-emission measures, including electric ones, is being promoted. This paper presents a method for scenario analysis applied to study the reduction of exhaust emissions by introducing electric vehicles in a selected city. The original scenario analyses relating to real problems faced by contemporary metropolitan areas are based on the VISUM tool (PTV Headquarters for Europe: PTV Planung Transport Verkehr AG, 76131 Karlsruhe, Germany). For the case study, the transport model of the city of Bielsko-Biala (Poland) was used to conduct experiments with different forms of participation of electric vehicles on the one hand and traffic restrictions for high emission vehicles on the other hand. Scenario analyses were conducted for various constraint options including inbound, outbound, and through traffic. Travel time for specific transport relations and the volume of harmful emissions were used as criteria for evaluating scenarios of limited accessibility to city zones for selected types of vehicles. The comparative analyses carried out showed that the introduction of electric vehicles in the inner city resulted in a significant reduction in the emission of harmful exhaust compounds and, consequently, in an increase in the area of clean air in the city. The case study and its results provide some valuable insights and may guide decision-makers in their actions to introduce both driving ban restrictions for high-emission vehicles and incentives for the use of electric vehicles for city residents.
Low-Rank Coal Supported Ni Catalysts for CO2 Methanation
Authors: Kim Soohyun , Yang Yunxia , Lippi Renata , Choi Hokyung , Kim Sangdo , Chun Donghyuk , Im Hyuk , Lee Sihyun , Yoo Jiho
Published: April 2021

As renewable energy source integration increases, P2G technology that can store surplus renewable power as methane is expected to expand. The development of a CO2 methanation catalyst, one of the core processes of the P2G concept, is being actively conducted. In this work, low-rank coal (LRC) was used as a catalyst support for CO2 methanation, as it can potentially enhance the diffusion and adsorption behavior by easily controlling the pore structure and composition. It can also improve the process efficiency owing to its simplicity (no pre-reduction step) and high thermal conductivity, compared to conventional metal oxide-supported catalysts. A screening of single metals (Ni, Co, Ru, Rh, and Pd) on LRC was performed, which showed that Ni was the most active. When Ni on the LRC catalyst was doped with a promoter (Ce and Mg), the CO2 conversion percentage increased by >10% compared to that of the single Ni catalyst. When the CO2 methanation activity was compared at 250–500 °C, the Ce-doped Ni/Eco and Mg-doped Ni/Eco catalysts showed similar or better activity than the commercial metal oxide-supported catalyst. In addition, the catalytic performance remained stable even after the test for an extended time (~200 h). The results of XRD, TEM, and TPR showed that highly efficient LRC-based CO2 methanation catalysts can be made when the metal dispersion and composition are modified.
Monitoring Radon Levels in Hospital Environments. Findings of a Preliminary Study in the University Hospital of Sassari, Italy
Authors: Deiana Giovanna , Dettori Marco , Masia Maria Dolores , Spano Antonio Lorenzo , Piana Andrea , Arghittu Antonella , Castiglia Paolo , Azara Antonio
Published: April 2021

Background: The aim of this preliminary study was to measure radon concentrations in a hospital in order to verify to what extent these concentrations depend on various environmental variables taken into consideration, and consequently to determine the urgency to implement mitigation actions. Methods: The rooms where the concentration of the gas was potentially highest were monitored. Investigators adopted a Continuous Radon Monitor testing device. Qualitative and normally distributed quantitative variables were summarised with absolute (relative) frequencies and means (standard deviations, SD), respectively. As regards environmental variables, the difference in radon concentrations was determined using the rank-based nonparametric Kruskal–Wallis H test and the Mann–Whitney U test. Results: All measurements, excluding the radiotherapy bunkers that showed high values due to irradiation of radiotherapy instruments, showed low radon levels, although there is currently no known safe level of radon exposure. In addition, high variability in radon concentration was found linked to various environmental and behavioural characteristics. Conclusions: The results on the variability of radon levels in hospital buildings highlighted the key role of monitoring activities on indoor air quality and, consequently, on the occupants’ health.
The Dynamic Impacts of the COVID-19 Pandemic on Log Prices in China: An Analysis Based on the TVP-VAR Model
Authors: Tao Chenlu , Diao Gang , Cheng Baodong
Published: April 2021

China’s wood industry is vulnerable to the COVID-19 pandemic since wood raw materials and sales of products are dependent on the international market. This study seeks to explore the speed of log price recovery under different control measures, and to perhaps find a better way to respond to the pandemic. With the daily data, we utilized the time-varying parameter autoregressive (TVP-VAR) model, which can incorporate structural changes in emergencies into the model through time-varying parameters, to estimate the dynamic impact of the pandemic on log prices at different time points. We found that the impact of the pandemic on oil prices and Renminbi exchange rate is synchronized with the severity of the pandemic, and the ascending in the exchange rate would lead to an increase in log prices, while oil prices would not. Moreover, the impulse response in June converged faster than in February 2020. Thus, partial quarantine is effective. However, the pandemic’s impact on log prices is not consistent with changes of the pandemic. After the pandemic eased in June 2020, the impact of the pandemic on log prices remained increasing. This means that the COVID-19 pandemic has long-term influences on the wood industry, and the work resumption was not smooth, thus the imbalance between supply and demand should be resolved as soon as possible. Therefore, it is necessary to promote the development of the domestic wood market and realize a “dual circulation” strategy as the pandemic becomes a “new normal”.
Oxidative Stability and Antioxidant Activity in Canned Eels: Effect of Processing and Filling Medium
Authors: Gómez-Limia Lucía , Sanmartín Nicolás Moya , Carballo Javier , Domínguez Rubén , Lorenzo José M. , Martínez Sidonia
Published: April 2021

The effect of canning and the use of different filling media (sunflower oil, olive oil, and spiced olive oil) on oxidation parameters (acidity, peroxide value (PV), and thiobarbituric acid reactive substances (TBARS) index), antioxidant capacity, and total phenol and vitamin E contents in eels was studied. A preliminary frying treatment caused a decrease in titratable acidity and an increase in TBARS, antioxidant capacity, and vitamin E in the eel muscle. During sterilization, TBARS also increased significantly. The magnitude of the changes depended on the filling medium. Storage also had a significant effect on oxidation parameters in eel muscle and in filling media. After one year of storage, the sunflower oil and canned eels packed in this oil presented higher antioxidant capacity and vitamin E content than olive oil, spiced olive oil, or canned eels packed in these oils. However, the total phenol contents were higher when olive oil or spiced olive oil were used as filling media. Despite the losses, the results show that the canning process and subsequent storage preserved a great part of the antioxidant capacity and vitamin E content of the filling medium, which is of interest to the consumer. Both sunflower oil and olive oil as filling media are of great nutritional interest.
cROStalk for Life: Uncovering ROS Signaling in Plants and Animal Systems, from Gametogenesis to Early Embryonic Development
Authors: Lodde Valentina , Morandini Piero , Costa Alex , Murgia Irene , Ezquer Ignacio
Published: April 2021

This review explores the role of reactive oxygen species (ROS)/Ca2+ in communication within reproductive structures in plants and animals. Many concepts have been described during the last years regarding how biosynthesis, generation products, antioxidant systems, and signal transduction involve ROS signaling, as well as its possible link with developmental processes and response to biotic and abiotic stresses. In this review, we first addressed classic key concepts in ROS and Ca2+ signaling in plants, both at the subcellular, cellular, and organ level. In the plant science field, during the last decades, new techniques have facilitated the in vivo monitoring of ROS signaling cascades. We will describe these powerful techniques in plants and compare them to those existing in animals. Development of new analytical techniques will facilitate the understanding of ROS signaling and their signal transduction pathways in plants and mammals. Many among those signaling pathways already have been studied in animals; therefore, a specific effort should be made to integrate this knowledge into plant biology. We here discuss examples of how changes in the ROS and Ca2+ signaling pathways can affect differentiation processes in plants, focusing specifically on reproductive processes where the ROS and Ca2+ signaling pathways influence the gametophyte functioning, sexual reproduction, and embryo formation in plants and animals. The study field regarding the role of ROS and Ca2+ in signal transduction is evolving continuously, which is why we reviewed the recent literature and propose here the potential targets affecting ROS in reproductive processes. We discuss the opportunities to integrate comparative developmental studies and experimental approaches into studies on the role of ROS/ Ca2+ in both plant and animal developmental biology studies, to further elucidate these crucial signaling pathways.
Genomic Structure of the Porcine CYP19 Locus and Expression of the CYP19A3 Paralog
Authors: Vanselow Jens , Conley Alan J. , Corbin Cynthia J. , Berger Trish
Published: April 2021

Proper, tissue-specific regulation of CYP19, the gene encoding aromatase, the key enzyme of estrogen synthesis, is essential for reproductive processes. Here, we analyzed transcriptional regulation of the porcine CYP19 in female and male gonads and brain by 5’RACE and RT-PCR and comprehensively mapped the pig CYP19 locus by in silico analysis. Our data revealed that the complete locus, including three paralogous copies, CYP19A1, CYP19A2 and CYP19A3, spans approximately 330 kb of the porcine chromosome 1. The locus also harbors the first exon of the Gliomedin gene (GLDN) in reverse orientation. Only transcripts of the CYP19A3 paralog were substantially expressed in gonads and hypothalamus. We identified CYP19A3-associated untranslated exons approximately 160 kb and 50 kb distal from the first codon. The 5´ untranslated regions of transcripts were derived from either a proximal or from one of these distal untranslated exons. Transcripts including only untranslated exons could be amplified from testis, thus suggesting long non-coding transcripts. The data revealed an additional layer of complexity in the regulation of the porcine CYP19 locus. Tissue-specific expression is not only achieved by tissue- and stage-specific expression of the three different CYP19 paralogs, but also by directing the expression of CYP19A3 from different, proximal and distal promoter regions.
Metagenomics Assessment of Soil Fertilization on the Chemotaxis and Disease Suppressive Genes Abundance in the Maize Rhizosphere
Authors: Enebe Matthew , Babalola Olubukola
Published: April 2021

Soil fertility is a function of the level of organic and inorganic substances present in the soil, and it influences the activities of soil-borne microbes, plant growth performance and a host of other beneficial ecological functions. In this metagenomics study, we evaluated the response of maize microbial functional gene diversity involved in chemotaxis, antibiotics, siderophores, and antifungals producing genes within the rhizosphere of maize plants under compost, inorganic fertilizer, and unfertilized conditions. The results show that fertilization treatments at higher compost manure and lower inorganic fertilizer doses as well as maize plants itself in the unfertilized soil through rhizosphere effects share similar influences on the abundance of chemotaxis, siderophores, antifungal, and antibiotics synthesizing genes present in the samples, while higher doses of inorganic fertilizer and lower compost manure treatments significantly repress these genes. The implication is for a disease suppressive soil to be achieved, soil fertilization with high doses of compost manure fertilizer treatments as well as lower inorganic fertilizer should be used to enrich soil fertility and boost the abundance of chemotaxis and disease suppressive genes. Maize crops also should be planted sole or intercropped with other crops to enhance the rhizosphere effect of these plants in promoting the expression and abundance of these beneficial genes in the soil.
Assessing Current Seismic Hazards in Irpinia Forty Years after the 1980 Earthquake: Merging Historical Seismicity and Satellite Data about Recent Ground Movements
Authors: Piombino Aldo , Bernardini Filippo , Farolfi Gregorio
Published: April 2021

Recently, a new strain rate map of Italy and the surrounding areas has been obtained by processing data acquired by the persistent scatterers (PS) of the synthetic aperture radar interferometry (InSAR) satellites—ERS and ENVISAT—between 1990 and 2012. This map clearly shows that there is a link between the strain rate and all the shallow earthquakes (less than 15 km deep) that occurred from 1990 to today, with their epicenters being placed only in high strain rate areas (e.g., Emilia plain, NW Tuscany, Central Apennines). However, the map also presents various regions with high strain rates but in which no damaging earthquakes have occurred since 1990. One of these regions is the Apennine sector, formed by Sannio and Irpinia. This area represents one of the most important seismic districts with a well-known and recorded seismicity from Roman times up to the present day. In our study, we merged historical records with new satellite techniques that allow for the precise determination of ground movements, and then derived physical dimensions, such as strain rate. In this way, we verified that in Irpinia, the occurrence of new strong shocks—forty years after one of the strongest known seismic events in the district that occurred on the 23 November 1980, measuring Mw 6.8—is still a realistic possibility. The reason for this is that, from 1990, only areas characterized by high strain rates have hosted significant earthquakes. This picture has been also confirmed by analyzing the historical catalog of events with seismic completeness for magnitude M ≥ 6 over the last four centuries. It is easy to see that strong seismic events with magnitude M ≥ 6 generally occurred at a relatively short time distance between one another, with a period of 200 years without strong earthquakes between the years 1732 and 1930. This aspect must be considered as very important from various points of view, particularly for civil protection plans, as well as civil engineering and urban planning development.
Three-Dimensional Reproducibility of the Soft Tissue Landmarks Taken by Structured-Light Facial Scanner in Accordance with the Head Position Change
Authors: Oh Seung-Hoon , Park Jung-Soo , Ryu Jae-Jun , Song In-Seok , Jung Seok-Ki
Published: April 2021

The aim of this study was to evaluate the three-dimensional reproducibility of the structured-light facial scanner according to the head position change. A mannequin head was used and angle of the mannequin’s axis-orbital plane to the true horizontal plane was adjusted to +10, +5, 0, −5, and −10°. Facial scanning was conducted 30 times, respectively, and 150 3D images were obtained. Reoriented landmarks of each group were compared and analyzed. Reproducibility decreased as the distance from the facial center increased. Additionally, the landmarks below showed lower reproducibility and higher dispersion than landmarks above. These differences occurred mainly in the anteroposterior direction as opposed to other directions. Positive inclination of the head position showed superior reproducibility compared to a negative inclination. This study showed that reproducibility of a structured-light scanner could be varied depending on the head position. Inaccuracies of landmarks in the anteroposterior direction are greater than in other directions. This means that evaluations of the profile using a structured-light scanner should be made carefully. Therefore, the proper head position should be set to ensure the accuracy of the image.
In-Hospital Outcomes in Patients Admitted to the Intensive Care Unit after a Return Visit to the Emergency Department
Authors: Lin Chun-Fu , Huang Yi-Syun , Tsai Ming-Ta , Wu Kuan-Han , Lin Chien-Fu , Chiu I-Min
Published: April 2021

Background: Intensive care unit (ICU) admission following a short-term emergency department (ED) revisit has been considered a particularly undesirable outcome among return-visit patients, although their in-hospital prognosis has not been discussed. We aimed to compare clinical outcomes between adult patients admitted to the ICU after unscheduled ED revisits and those admitted during index ED visits. Method: This retrospective study was conducted at two tertiary medical centers in Taiwan from 1 January 2016 to 31 December 2017. All adult non-trauma patients admitted to the ICU directly via the ED during the study period were included and divided into two comparison groups: patients admitted to the ICU during index ED visits and those admitted to the ICU during return ED visits. The outcomes of interest included in-hospital mortality, mechanical ventilation (MV) support, profound shock, hospital length of stay (HLOS), and total medical cost. Results: Altogether, 12,075 patients with a mean (standard deviation) age of 64.6 (15.7) years were included. Among these, 5.3% were admitted to the ICU following a return ED visit within 14 days and 3.1% were admitted following a return ED visit within 7 days. After adjusting for confounding factors for multivariate regression analysis, ICU admission following an ED revisit within 14 days was not associated with an increased mortality rate (adjusted odds ratio (aOR): 1.08, 95% confidence interval (CI): 0.89 to 1.32), MV support (aOR: 1.06, 95% CI: 0.89 to 1.26), profound shock (aOR: 0.99, 95% CI: 0.84 to 1.18), prolonged HLOS (difference: 0.04 days, 95% CI: −1.02 to 1.09), and increased total medical cost (difference: USD 361, 95% CI: −303 to 1025). Similar results were observed after the regression analysis in patients that had a 7-day return visit. Conclusion: ICU admission following a return ED visit was not associated with major in-hospital outcomes including mortality, MV support, shock, increased HLOS, or medical cost. Although ICU admissions following ED revisits are considered serious adverse events, they may not indicate poor prognosis in ED practice.