bluuebunny/crossref_metadata_2025 huggingface dataset:
Epidermal Fatty Acid-Binding Protein: A Novel Marker in the Diagnosis of Dry Eye Disease in Sjögren Syndrome
Authors: Shinzawa Megumi , Dogru Murat , Den Seika , Ichijima Takehiro , Higa Kazunari , Kojima Takashi , Seta Noriyuki , Nomura Takeshi , Tsubota Kazuo , Shimazaki Jun
Published: November 2018

Purpose: Sjögren syndrome (SS) is a chronic inflammatory autoimmune disease of the lacrimal and salivary glands. This study compared the concentrations of epidermal fatty-acid binding protein (E-FABP) in the saliva, serum, and tears of SS patients with dry eye and dry mouth, with those of healthy adults to investigate the usefulness of E-FABP as a diagnostic marker for SS. Design: Prospective, observational case series. Participants: The subjects were 11 new patients with untreated Sjogren syndrome and 12 healthy control individuals. Methods: The diagnosis of SS was in accordance with the Ministry of Health, Labour and Welfare (Japan) Diagnostic Criteria (1999). Saliva, serum, and tear specimens were collected during internal medicine, dental, and ophthalmological examinations. The ophthalmological tests included the Dry Eye-related Quality of life Score (DEQS), tear break-up time (BUT), vital staining with fluorescein (FS) and lissamine green (LG), and the Schirmer test-1. The E-FABP concentration in the tears, saliva, and serum was measured by enzyme-linked immunosorbent assay (ELISA). Main outcome measure: The E-FABP concentrations were compared between patients and controls. Results: There were significant differences between the patient and healthy control groups in all ophthalmological test results. There were no significant differences between the groups in the E-FABP concentrations in the saliva (p = 0.1513) or the serum (p = 0.4799), but the E-FABP concentration in the tears significantly differed between groups. The E-FABP concentration in tears tended to be significantly lower in patients with SS (mean, 323.5 ± 325.6 pg/mL) than healthy control subjects (mean, 4076 pg/mL; p = 0.0136). The E-FABP concentration in tears significantly correlated with the results of dry eye parameters. Conclusion: The E-FABP concentration in tears appears to be related to ocular surface epithelial damage and tear stability and may be a promising novel biomarker in the diagnosis of SS.
Elucidating the Beneficial Role of PPAR Agonists in Cardiac Diseases
Authors: Khuchua Zaza , Glukhov Aleksandr I. , Strauss Arnold W. , Javadov Sabzali
Published: November 2018

Peroxisome proliferator-activated receptors (PPARs) are nuclear hormone receptors that bind to DNA and regulate transcription of genes involved in lipid and glucose metabolism. A growing number of studies provide strong evidence that PPARs are the promising pharmacological targets for therapeutic intervention in various diseases including cardiovascular disorders caused by compromised energy metabolism. PPAR agonists have been widely used for decades as lipid-lowering and anti-inflammatory drugs. Existing studies are mainly focused on the anti-atherosclerotic effects of PPAR agonists; however, their role in the maintenance of cellular bioenergetics remains unclear. Recent studies on animal models and patients suggest that PPAR agonists can normalize lipid metabolism by stimulating fatty acid oxidation. These studies indicate the importance of elucidation of PPAR agonists as potential pharmacological agents for protection of the heart from energy deprivation. Here, we summarize and provide a comprehensive analysis of previous studies on the role of PPARs in the heart under normal and pathological conditions. In addition, the review discusses the PPARs as a therapeutic target and the beneficial effects of PPAR agonists, particularly bezafibrate, to attenuate cardiomyopathy and heart failure in patients and animal models.
Dynamic Collision Behavior Between Osteoblasts and Tumor Cells Regulates the Disordered Arrangement of Collagen Fiber/Apatite Crystals in Metastasized Bone
Authors: Matsugaki Aira , Harada Tatsuki , Kimura Yumi , Sekita Aiko , Nakano Takayoshi
Published: November 2018

Bone metastasis is one of the most intractable bone diseases; it is accompanied with a severe mechanical dysfunction of bone tissue. We recently discovered that the disorganized collagen/apatite microstructure in cancer-bearing bone is a dominant determinant of the disruption of bone mechanical function; disordered osteoblast arrangement was found to be one of the principal determinants of the deteriorated collagen/apatite microstructure. However, the precise molecular mechanisms regulating the disordered osteoblast arrangement triggered by cancer invasion are not yet understood. Herein, we demonstrate a significant disorganization of bone tissue anisotropy in metastasized bone in our novel ex vivo metastasis model. Further, we propose a novel mechanism underlying the disorganization of a metastasized bone matrix: A dynamic collision behavior between tumor cells and osteoblasts disturbs the osteoblast arrangement along the collagen substrate.
Proteomic Analysis of Plasma Membrane Proteins of Antler Stem Cells Using Label-Free LC–MS/MS
Authors: Wang Datao , Ba Hengxing , Li Chenguang , Zhao Quanmin , Li Chunyi
Published: November 2018

Deer antlers are unusual mammalian organs that can fully regenerate after annual shedding. Stem cells resident in the pedicle periosteum (PPCs) provide the main cell source for antler regeneration. Central to various cellular processes are plasma membrane proteins, but the expression of these proteins has not been well documented in antler regeneration. In the present study, plasma membrane proteins of PPCs and facial periosteal cells (FPCs) were analyzed using label-free liquid chromatography–mass spetrometry (LC–MS/MS). A total of 1739 proteins were identified. Of these proteins, 53 were found solely in the PPCs, 100 solely in the FPCs, and 1576 co-existed in both PPCs and FPCs; and 39 were significantly up-regulated in PPCs and 49 up-regulated in FPCs. In total, 226 gene ontology (GO) terms were significantly enriched from the differentially expressed proteins (DEPs). Five clusters of biological processes from these GO terms comprised responses to external stimuli, signal transduction, membrane transport, regulation of tissue regeneration, and protein modification processes. Further studies are required to demonstrate the relevancy of these DEPs in antler stem cell biology and antler regeneration.
Room Temperature Ni(II) Catalyzed Hydrophosphination and Cyclotrimerization of Alkynes
Authors: Webster Ruth L.
Published: November 2018

The catalytic activity of nickel complexes in hydrophosphination involving secondary phosphines is not a commonly studied transformation. Beyond a small number of stand-out examples, many reports in the literature focus on the use of simple nickel salts. β-Diketiminates have been proven to be incredibly effective ligands for catalysis using a range of metal centers. This synthetic study investigates the catalytic ability of a Ni(II) β-diketiminate complex in the hydrophosphination of alkenes and alkynes, with a serendipitous discovery of its ability to effect alkyne cyclotrimerization and phosphine dehydrocoupling.
The Influence of Upper and Lower Extremity Strength on Performance-Based Sarcopenia Assessment Tests
Authors: Harris-Love Michael O. , Benson Kimberly , Leasure Erin , Adams Bernadette , McIntosh Valerie
Published: November 2018

The optimal management of sarcopenia requires appropriate endpoint measures to determine intervention efficacy. While hand grip strength is a predictor of morbidity and mortality, lower extremity strength may be better associated with functional activities in comparison to hand grip strength. The purpose of our study was to examine the comparative association of upper and lower extremity strength with common measures of physical performance in older adults. Thirty community-dwelling men, aged 62.5 ± 9.2 years, completed body composition analysis, quantitative strength testing, and performance-based tests of functional status. Hand grip force values were not significantly associated with knee extensor or flexor torque values (p > 0.05). Hand grip force was only associated with fast gait speed, while knee extensor torque at 60°/s was the only variable significantly associated across all functional outcome measures: customary gait speed, fast gait speed, sit to stand time, and the Physical Performance Test (p < 0.02). Hand grip strength was not a proxy measure of lower extremity strength as assessed in this study. Overall, lower extremity muscle strength values had the strongest associations with participant functional performance. Lower extremity strength testing may provide additional value as an endpoint measure in the assessment and clinical management of sarcopenia.
Laser Scanners for High-Quality 3D and IR Imaging in Cultural Heritage Monitoring and Documentation
Authors: Ceccarelli Sofia , Guarneri Massimiliano , Ferri de Collibus Mario , Francucci Massimo , Ciaffi Massimiliano , Danielis Alessandro
Published: November 2018

Digital tools as 3D (three-dimensional) modelling and imaging techniques are having an increasing role in many applicative fields, thanks to some significative features, such as their powerful communicative capacity, versatility of the results and non-invasiveness. These properties are very important in cultural heritage, and modern methodologies provide an efficient means for analyzing deeply and virtually rendering artworks without contact or damage. In this paper, we present two laser scanner prototypes based on the Imaging Topological Radar (ITR) technology developed at the ENEA Research Center of Frascati (RM, Italy) to obtain 3D models and IR images of medium/large targets with the use of laser sources without the need for scaffolding and independently from illumination conditions. The RGB-ITR (Red Green Blue-ITR) scanner employs three wavelengths in the visible range for three-dimensional color digitalization up to 30 m, while the IR-ITR (Infrared-ITR) system allows for layering inspection using one IR source for analyses. The functionalities and operability of the two systems are presented by showing the results of several case studies and laboratory tests.
Impact of Precision Medicine on Drug Repositioning and Pricing: A Too Small to Thrive Crisis
Authors: Tiriveedhi Venkataswarup
Published: November 2018

The pricing of targeted medicines continues to be a major area of contention in healthcare economics. This issue is further complicated by redefining the role of molecular testing in precision medicine. Currently, whilst pricing of clinical laboratory diagnostics is cost-based, drug pricing is value-based. The pricing for molecular testing is under pressure to change the traditional business model, for it has a critical subsidiary role in determining the final value of targeted medicines. The market size for drugs is reduced by molecular testing when patients with the same disease are stratified based on their genetics, it is critical to determine the value of this new enhanced drug specificity to realize its full pricing potential. However, these value-based pricing strategies require a careful understanding of changing market conditions, especially, in the context of stratified patient segments made possible by precision medicine. In this article, we discuss the various factors impacting pricing decisions, and consider evolving economic trends in precision medicine.
Language Contact in Modern Uyghur. By Aminem Memtimin. Turcologica 108, Harrassowitz Verlag: Wiesbaden, Germany, 2016, 245p.; ISBN: 978-3-447-10631-3
Authors: Cabras Giulia
Published: November 2018

The study of language contact can be approached from different perspectives, such as in terms of diachronic development, synchronic variation, structural change, or social meanings. [...]
[O-Isopropyl-N-(4-nitrophenyl)thiocarbamato-κS]-(tri-4-tolylphosphine-κP)gold(I)
Authors: Yeo Chien Ing , Tiekink Edward R. T.
Published: November 2018

The synthesis, spectroscopic characterization and X-ray crystal structure of the title compound, (4-tolyl)3PAu[SC(O-i-Pr)=NC6H4NO2-4] (1) are described. Spectroscopy exhibited the expected features confirming the formation of the compound. The molecular structure of 1 confirms the expected linear P–Au–S coordination geometry defined by thiolate-S and phosphane-P atoms. The nearly 7° deviation from linearity is ascribed to the close approach of the imine-bound phenyl group, indicative of a semi-localized Au…π(arene) interaction. The three-dimensional molecular packing is consolidated by methyl- and tolyl-C–H…O(nitro) and tolyl-C–H…π(tolyl) interactions.
Preparation and Properties Analysis of Chlorinated Butyl Rubber (CIIR)/Organic Diatomite Damping Composites
Authors: Sheng Zeyuan , Yang Siyuan , Wang Jincheng , Lu Yao , Tang Keya , Song Shiqiang
Published: November 2018

In this work, a novel type of diatomite was prepared with a limited content of hindered phenol groups grafted on its hydrophobic surface. The obtained samples were characterized for their surface groups, particle morphology, pore structure, and thermal behaviors. Then, modified diatomite (MDT) was used in preparation of reinforced chlorinated butyl rubber (CIIR) composites by mechanical blending method. The powder of MDT can be uniformly dispersed in CIIR matrices and the compatibility was good. In addition, the MDT showed a positive effect on damping performance of CIIR composites. A blending ratio of CIIR/MDT = 100/10 presented the best damping performance and the damping temperature range (tan δ > 0.7) was extended from 60 to 70 °C. The variable temperature FTIR spectra showed the presence of hydrogen bonds between the hydroxyl groups and chloride atoms in the CIIR matrices, and a blue shift exhibited when these hydrogen bonds were dissociated. Hence, these CIIR composites provided good damping behaviors and supplied a novel and promising way for preparation of high damping rubber composites with broad temperature ranges.
Blending In Situ Polyurethane-Urea with Different Kinds of Rubber: Performance and Compatibility Aspects
Authors: Tahir Muhammad , Heinrich Gert , Mahmood Nasir , Boldt Regine , Wießner Sven , Stöckelhuber Klaus Werner
Published: November 2018

Specific physical and reactive compatibilization strategies are applied to enhance the interfacial adhesion and mechanical properties of heterogeneous polymer blends. Another pertinent challenge is the need of energy-intensive blending methods to blend high-tech polymers such as the blending of a pre-made hard polyurethane (-urea) with rubbers. We developed and investigated a reactive blending method to prepare the outstanding blends based on polyurethane-urea and rubbers at a low blending temperature and without any interfacial compatibilizing agent. In this study, the polyurethane-urea (PUU) was synthesized via the methylene diphenyl diisocyanate end-capped prepolymer and m-phenylene diamine based precursor route during blending at 100 °C with polar (carboxylated nitrile rubber (XNBR) and chloroprene rubber (CR)) and non-polar (natural rubber (NR), styrene butadiene rubber (sSBR), and ethylene propylene butadiene rubber (EPDM)) rubbers. We found that the in situ PUU reinforces the tensile response at low strain region and the dynamic-mechanical response up to 150 °C in the case of all used rubbers. Scanning electron microscopy reveals a stronger rubber/PUU interface, which promotes an effective stress transfer between the blend phases. Furthermore, energy filtered transmission electron microscopy (EFTEM) based elemental carbon map identifies an interphase region along the interface between the nitrile rubber and in situ PUU phases of this exemplary blend type.
A Numerical Method to Model Non-linear Damping Behaviour of Martensitic Shape Memory Alloys
Authors: Haghdoust Pouya , Lo Conte Antonietta , Cinquemani Simone , Lecis Nora
Published: November 2018

This article investigates the efficiency of hybridizing composites with thin layers of martensitic shape memory alloys for improvement of damping. The non-linear damping behaviour of martensitic shape memory alloys is simulated using a modified version of Masing’s rules. The model was implemented in a user subroutine of a finite element code, and validated by a numerical simulation of experimental hysteresis loops at different maximum strain amplitudes. The experimental free decay of hybridized glass fiber reinforced polymer beams was simulated using the finite element model, including the validated model of the investigated materials. The amplitude-dependent damping of the hybrid beams in free decay was reproduced successfully in the numerical analysis and it was proven that the hybridization technique is efficient for improvement of damping.
Graphene-Modulated Removal Performance of Nitrogen and Phosphorus Pollutants in a Sequencing Batch Chlorella Reactor
Authors: Xia Gonghan , Xu Wenlai , Fang Qinglin , Mou Zishen , Pan Zhicheng
Published: November 2018

In this work, the influence of graphene on nitrogen and phosphorus in a batch Chlorella reactor was studied. The impact of graphene on the removal performance of Chlorella was investigated in a home-built sewage treatment system with seven identical sequencing batch Chlorella reactors with graphene contents of 0 mg/L (T1), 0.05 mg/L (T2), 0.1 mg/L (T3), 0.2 mg/L (T4), 0.4 mg/L (T5), 0.8 mg/L (T6) and 10 mg/L (T7). The influence of graphene concentration and reaction time on the pollutant removal performance was studied. The malondialdehyde (MDA) and total superoxide dismutase (SOD) concentrations in each reactor were measured, and optical microscopy and scanning electron microscopy (SEM) characterizations were performed to determine the related mechanism. The results show that after 168 h, the total nitrogen (TN), ammonia nitrogen (AN) and total phosphorus (TP) removal rates of reactors T1–T7 become stable, and the TN, AN and TP removal rates were gradually reduced with increasing graphene concentration. At 96 h, the concentrations of both MDA and SOD in T1–T7 gradually increased as the graphene concentration increased. In optical microscopy and SEM measurements, it was found that graphene was adsorbed on the surface of Chlorella, and entered Chlorella cells, deforming and reducing Chlorella. Through the blood plate count method, we estimated an average Chlorella reduction of 16%. According to the water quality and microscopic experiments, it can be concluded that the addition of graphene causes oxidative damage to microalgae and destruction of the Chlorella cell wall and cell membrane, inhibiting the nitrogen and phosphorus removal in Chlorella reactors. This study provides theoretical and practical support for the safe use of graphene.
Comparison of Microstructure and Properties of In-Situ TiN- and WC-Reinforced NiCrBSi Composite Coatings Prepared by Plasma Spraying
Authors: Zhu Linlin , Wang Jing , Deng Xianqiang , Dong Yanchun , Yang Yong , Li Dongyang
Published: November 2018

In this study, NiCrBSi-30 wt.% TiN composite (NTC) coating was produced on carbon steel via plasma spraying, with NiCrBSi-30 wt.% WC composite (NWC) coating as the comparison object. The microstructure and phase constituents of the composite coatings were characterized using scanning electron microscopy (SEM) coupled with energy dispersive spectroscopy (EDS) techniques, transmission electron microscopy (TEM) and x-ray diffraction (XRD). Atomic force microscopy (AFM) was used to measure electronic work functions. The microhardness and wear performance of coatings were also investigated. The average microhardness of the NTC and NWC coatings was 1000 HV and 850 HV, respectively. In addition, the NTC coating had a wear volume loss of 0.8118 mm3, less than 1.4772 mm3, the volume loss of the NWC coating. This was due to the presence of TiN in the form of nanograins in the composite coating and tighter binding to the matrix.
Assessments of Secondary Reinforcement of Epoxy Matrix-Glass Fibre Composite Laminates through Nanosilica (SiO2)
Authors: Lazar Prince Jeya Lal , Sengottuvelu Ramesh , Natarajan Elango
Published: November 2018

The principal objective of this research work was to investigate the results of impregnating epoxy matrix-glass fibre composite laminates with nanosilica as secondary reinforcement. 0.5, 0.75, 1 and 3 wt% nanosilica was used and thereafter properties of composites were assessed through tensile, three point bending, quasi static indentation tests and dynamic mechanical analysis. Scanning electron microscope examinations were done on fracture surfaces and failure modes were analyzed. The internal failures of the composite due to quasi-static indentation were evaluated through C-Scan. Among samples of different weight fractions, 0.75 wt% nanosilica reinforced composite laminates exhibited substantial increase of 42% in tensile strength and 39.46% in flexural strength. The reduction in glass transition temperature (Tg), increase in storage modulus (E′), loss modulus (E″) and damping factor (tan δ) were also observed. Quasi-static indentation assessments revealed that energy absorption property was enhanced significantly by 53.97%. Hence nanosilica up to 0.75 wt% can be used as a potential candidate for secondary reinforcement in epoxy composite laminates.
Self-Assembly Properties of Amphiphilic Iron(III) Spin Crossover Complexes in Water and at the Air–Water Interface
Authors: Martinho Paulo N. , Kühne Irina A. , Gildea Brendan , McKerr George , O’Hagan Barry , Keyes Tia E. , Lemma Tibebe , Gandolfi Claudio , Albrecht Martin , Morgan Grace G.
Published: November 2018

The assembly properties of three known spin crossover iron(III) complexes 1–3, at the air–water interface, are reported. All three complexes are amphiphiles, each bearing a pair of Cn alkyl chains on the polyamino Schiff base sal2trien ligand (n = 6, 12, or 18). Complex 1 is water-soluble but complexes 2 and 3 form Langmuir films, and attempts were made to transfer the film of the C18 complex 3 to a glass surface. The nature of the assembly of more concentrated solutions of 3 in water was investigated by light scattering, cryo-SEM (scanning electron microscopy), and TEM (transmission electron microscopy), all of which indicated nanoparticle formation. Lyophilization of the assembly of complex 3 in water yielded a powder with a markedly different magnetic profile from the powder recovered from the initial synthesis, notably, the spin crossover was almost completely quenched, and the thermal behavior was predominantly low spin, suggesting that nanoparticle formation traps the system in one spin state.
Bipolar Fuzzy UP-Algebras
Authors: Kawila Korawut , Udomsetchai Chaiphon , Iampan Aiyared
Published: November 2018

In this paper, we apply the notion of bipolar-valued fuzzy set to UP-algebras. We introduce the notions of bipolar fuzzy UP-subalgebras (resp., bipolar fuzzy UP-filters, bipolar fuzzy UP-ideals, and bipolar fuzzy strongly UP-ideals) of UP-algebras and prove their generalizations. We provide a condition for a bipolar fuzzy UP-filter to be a bipolar fuzzy UP-ideal. Further, we discuss the relation between bipolar fuzzy UP-subalgebras (resp., bipolar fuzzy UP-filters, bipolar fuzzy UP-ideals, and bipolar fuzzy strongly UP-ideals) and their level cuts.
Astaxanthin Prevents Human Papillomavirus L1 Protein Binding in Human Sperm Membranes
Authors: Donà Gabriella , Andrisani Alessandra , Tibaldi Elena , Brunati Anna Maria , Sabbadin Chiara , Armanini Decio , Ambrosini Guido , Ragazzi Eugenio , Bordin Luciana
Published: November 2018

Astaxanthin (Asta), red pigment of the carotenoid family, is known for its anti-oxidant, anti-cancer, anti-diabetic, and anti-inflammatory properties. In this study, we evaluated the effects of Asta on isolated human sperm in the presence of human papillomavirus (HPV) 16 capsid protein, L1. Sperm, purified by gradient separation, were treated with HPV16-L1 in both a dose and time-dependent manner in the absence or presence of 30 min-Asta pre-incubation. Effects of HPV16-L1 alone after Asta pre-incubation were evaluated by rafts (CTB) and Lyn dislocation, Tyr-phosphorylation (Tyr-P) of the head, percentages of acrosome-reacted cells (ARC) and endogenous reactive oxygen species (ROS) generation. Sperm membranes were also analyzed for the HPV16-L1 content. Results show that HPV16-L1 drastically reduced membrane rearrangement with percentage of sperm showing head CTB and Lyn displacement decreasing from 72% to 15.8%, and from 63.1% to 13.9%, respectively. Accordingly, both Tyr-P of the head and ARC decreased from 68.4% to 10.2%, and from 65.7% to 14.6%, respectively. Asta pre-incubation prevented this drop and restored values of the percentage of ARC up to 40.8%. No alteration was found in either the ROS generation curve or sperm motility. In conclusion, Asta is able to preserve sperm by reducing the amount of HPV16-L1 bound onto membranes.
Fishing for Targets of Alien Metabolites: A Novel Peroxisome Proliferator-Activated Receptor (PPAR) Agonist from a Marine Pest
Authors: Vitale Rosa , D'Aniello Enrico , Gorbi Stefania , Martella Andrea , Silvestri Cristoforo , Giuliani Maria , Fellous Tariq , Gentile Alessandra , Carbone Marianna , Cutignano Adele , Grauso Laura , Magliozzi Laura , Polese Gianluca , D'Aniello Biagio , Defranoux Fanny , Felline Serena , Terlizzi Antonio , Calignano Antonio , Regoli Francesco , Di Marzo Vincenzo , Amodeo Pietro , Mollo Ernesto
Published: November 2018

Although the chemical warfare between invasive and native species has become a central problem in invasion biology, the molecular mechanisms by which bioactive metabolites from invasive pests influence local communities remain poorly characterized. This study demonstrates that the alkaloid caulerpin (CAU)—a bioactive component of the green alga Caulerpa cylindracea that has invaded the entire Mediterranean basin—is an agonist of peroxisome proliferator-activated receptors (PPARs). Our interdisciplinary study started with the in silico prediction of the ligand-protein interaction, which was then validated by in vivo, ex vivo and in vitro assays. On the basis of these results, we candidate CAU as a causal factor of the metabolic and behavioural disorders observed in Diplodus sargus, a native edible fish of high ecological and commercial relevance, feeding on C. cylindracea. Moreover, given the considerable interest in PPAR activators for the treatment of relevant human diseases, our findings are also discussed in terms of a possible nutraceutical/pharmacological valorisation of the invasive algal biomasses, supporting an innovative strategy for conserving biodiversity as an alternative to unrealistic campaigns for the eradication of invasive pests.
Limonoids Containing a C1–O–C29 Moiety: Isolation, Structural Modification, and Antiviral Activity
Authors: Ren Jing-Ling , Zou Xiao-Peng , Li Wan-Shan , Shen Li , Wu Jun
Published: November 2018

Five new limonoids named thaigranatins A–E (1–5), containing a C1–O–C29 moiety, were isolated from seeds of the Thai Xylocarpus granatum, collected at the mangrove swamp of Trang Province, together with the known limonoid, granatumin L (6). The structures of these compounds were established by HR-ESIMS and extensive NMR spectroscopic data. The absolute configuration of 1 was unequivocally determined by single-crystal X-ray diffraction analysis, conducted with Cu Kα radiation; whereas that of 2 or 6 was established to be the same as that of 1 by the similarity of their electronic circular dichroism (ECD) spectra. In view of the marked antiviral activity of 6, its structure was modified via hydrolysis with alkaline KOH, esterification with diazomethane and various organic acids, and oximization with hydroxyamine. Finally, 18 derivatives, viz. 7–10, 8a–8i, 9a–9b, and 10a–10c, were obtained. In vitro antiviral activities of these derivatives against human immunodeficiency virus 1 (HIV-1) and influenza A virus (IAV) were evaluated. Most notably, 8i exhibited marked inhibitory activity against HIV-1 with an IC50 value of 15.98 ± 6.87 μM and a CC50 value greater than 100.0 μM; whereas 10b showed significant inhibitory activity against IAV with an IC50 value of 14.02 ± 3.54 μM and a CC50 value greater than 100.0 μM.
Micro-Galvanic Corrosion of Steam Generator Materials within Pores of Magnetite Flakes in Alkaline Solutions
Authors: Jeon Soon-Hyeok , Song Geun Dong , Hur Do Haeng
Published: November 2018

In secondary coolant system of the pressurized water reactors, the reduced corrosion products such as metallic Cu and Pb particles were accumulated in the pores of the magnetite flakes and electrically contacted to the steam generator materials. The micro-galvanic corrosion behavior of steam generator materials (steam generator tube materials: Alloy 600 and Alloy 690, steam generator tube sheet materials: SA508 Gr.3) contacted to the corrosion products (magnetite, Cu, and Pb) was investigated in an alkaline solution. The steam generator materials considered in this study were all the anodic elements of the galvanic pair because their corrosion potentials were lower than those of the corrosion products. The corrosion rate of the steam generator materials was increased by the galvanic coupling with the each corrosion products, and was more accelerated with increasing the area ratio of the corrosion products to the steam generator materials. Among the corrosion products, Cu has the largest galvanic effect on steam generator materials in the pores when area ratio of cathode to anode is 10.
Tensile Deformation Behavior of Medium Manganese Steels with High Carbon Concentrations and Austenitic Microstructures
Authors: Luan Guoqing , Volkova Olena , Mola Javad
Published: November 2018

Tensile properties of the Fe–1.44C–8Mn–1.9Al (mass %) steel with a fully austenitic microstructure, obtained by rapid quenching from the austenite range, was determined at room temperature. Tensile tests were performed using specimens prepared in two different routes involving an exchanged sequence of machining and heat treatment as the last processing steps prior to tensile tests. In spite of the occurrence of deformation twinning, total tensile elongations in both processing routes remained below 18%. Abrupt stress drops during tensile tests suggested the occurrence of discontinuities in tensile specimens. Detailed examination of the fracture surface indicated a mixed intergranular–transgranular fracture mode. Furthermore, a high density of surface cracks was observed near the outer surface in the gauge section of fractured tensile specimens. The origin of surface cracks could not be identified. The coincidence of surface cracks with the grain boundaries, especially those nearly perpendicular to the tensile direction, is thought to be responsible for the accelerated grain boundary decohesion and the premature fracture of tensile specimens.
Effect of Carbon Content in Retained Austenite on the Dynamic Tensile Behavior of Nanostructured Bainitic Steel
Authors: Zhou Wen , Hou Tingping , Zhang Cong , Zhong Lei , Wu Kaiming
Published: November 2018

Results of dynamic tensile testing of three-step low-temperature-transformed nanostructured bainitic steel and quenching and partitioning martensitic steel at different strain rates (0.1–500 s−1) are reported here. The results showed that the high carbon film-like austenite was much more stable than the low carbon blocky austenite during deformation. The nanostructured bainite steel exhibited the more remarkable dynamic tensile properties due to the better transformation-induced plasticity effect and strain rate hardening effect exhibited by stable film-like retained austenite. The big gap in engineering stress and strain curves occurred at a higher strain rate (100–200 s−1) for the nanostructured bainite steel because of the better stability of film-like austenite. Therefore, the present study is able to assist in explaining the effect of carbon content in retained austenite on the dynamic tensile properties and understanding the microstructure property relationship in complex steels.
An Improved UU-MESFET with High Power Added Efficiency
Authors: Jia Hujun , Hu Mei , Zhu Shunwei
Published: November 2018

An improved ultrahigh upper gate 4H-SiC metal semiconductor field effect transistor (IUU-MESFET) is proposed in this paper. The structure is obtained by modifying the ultrahigh upper gate height h of the ultrahigh upper gate 4H-SiC metal semiconductor field effect transistor (UU-MESFET) structure, and the h is 0.1 μm and 0.2 μm for the IUU-MESFET and UU-MESFET, respectively. Compared with the UU-MESFET, the IUU-MESFET structure has a greater threshold voltage and trans-conductance, and smaller breakdown voltage and saturation drain current, and when the ultrahigh upper gate height h is 0.1 μm, the relationship between these parameters is balanced, so as to solve the contradictory relationship that these parameters cannot be improved simultaneously. Therefore, the power added efficiency (PAE) of the IUU-MESFET structure is increased from 60.16% to 70.99% compared with the UU-MESFET, and advanced by 18%.
Research on the Relationship between Cutting Force and Machined Surface Quality in Micro Ball End-Milling of Potassium Dihydrogen Phosphate Crystal
Authors: Chen Ni , Wu Chunya , Chen Mingjun , Li Liang , He Ning
Published: November 2018

Potassium dihydrogen phosphate (KDP or KH2PO4) crystal is widely used as terminal frequency converters in inertial confinement fusion (ICF). However, KDP crystal is a typical difficult-to-cut optical crystal with the characteristic of soft-brittle. In this work, the relationship between cutting force and processed surface quality in micro ball end-milling of KDP crystal with various depth of cut and spindle speed is studied by carried out the micro-milling experiments. Fast Fourier Transform (FFT) algorithm is used to diagnose the recorded cutting force. The periodic change of cutting force and the cutting force after filtering noises can be got through FFT analysis. Through calculating the correlation coefficients between the static component of thrust force and roughness value Ra of machined grooves, as well as the peak-valley (P-V) value of thrust force and dimensional error of machined grooves, the roughness value Ra and dimensional error of machined grooves would be predicted by monitoring the static component and P-V value of the thrust force, respectively. The relatively large spindle speed helps to reduce the roughness value Ra. The spindle speed with moderate value is recommended to reduce the dimensional error of machined groove because the dimensional error of machined groove will increase when the spindle speed is small enough (causing brittle cutting) or large enough (reducing cutting stability).
Development of Flexible Robot Skin for Safe and Natural Human–Robot Collaboration
Authors: Pang Gaoyang , Deng Jia , Wang Fangjinhua , Zhang Junhui , Pang Zhibo , Yang Geng
Published: November 2018

For industrial manufacturing, industrial robots are required to work together with human counterparts on certain special occasions, where human workers share their skills with robots. Intuitive human–robot interaction brings increasing safety challenges, which can be properly addressed by using sensor-based active control technology. In this article, we designed and fabricated a three-dimensional flexible robot skin made by the piezoresistive nanocomposite based on the need for enhancement of the security performance of the collaborative robot. The robot skin endowed the YuMi robot with a tactile perception like human skin. The developed sensing unit in the robot skin showed the one-to-one correspondence between force input and resistance output (percentage change in impedance) in the range of 0–6.5 N. Furthermore, the calibration result indicated that the developed sensing unit is capable of offering a maximum force sensitivity (percentage change in impedance per Newton force) of 18.83% N−1 when loaded with an external force of 6.5 N. The fabricated sensing unit showed good reproducibility after loading with cyclic force (0–5.5 N) under a frequency of 0.65 Hz for 3500 cycles. In addition, to suppress the bypass crosstalk in robot skin, we designed a readout circuit for sampling tactile data. Moreover, experiments were conducted to estimate the contact/collision force between the object and the robot in a real-time manner. The experiment results showed that the implemented robot skin can provide an efficient approach for natural and secure human–robot interaction.
Mineral Assemblages, Textures and In Situ Sulphur Isotope Geochemistry of Sulphide Mineralization from the Cyprus-Type Ice Volcanogenic Massive Sulphide (VMS) Deposit, Yukon, Canada
Authors: McDonald Mervin J. , Piercey Stephen J. , Layne Graham D. , Pigage Lee C. , Piercey Glenn
Published: November 2018

The Permian (~273–274 Ma) Ice volcanogenic massive sulphide (VMS) deposit represents a mound shaped Cyprus (mafic)-type VMS deposit (~4.5 Mt @ 1.5% Cu) hosted in basaltic rocks of Slide Mountain terrane. The deposit consists of massive sulphides that are underlain by a chlorite-sulphide-hematite-rich stringer pipe, and overlain by a hematite-(pyrite)-rich exhalative chert. The sulphides are divided into five facies: (1) pyrite-rich; (2) pyrite-bornite-rich; (3) pyrite-chalcopyrite-rich; (4) hematite-pyrite; and (5) stringer sulphide. The sulphides have a distinct paragenetic and textural evolution in the massive sulphide that reflect: (1) an early, low temperature stage (<250 °C dominated by Fe-Zn-Cu-rich mineralization; (2) an intermediate, high temperature stage (>300 °C) dominated by Cu-Fe-rich mineralization; and (3) a late, low temperature phase (<150 °C) dominated by Fe-rich mineralization. In situ sulphur isotope data pyrite and chalcopyrite (by secondary ion mass spectrometry (SIMS)) range from δ34S = +1.8‰ to +8.2‰, but vary as a function of paragenesis and temperature of deposition. Both early and late forming sulfides were dominated by sulphur from partial thermochemical sulphate reduction (TSR) of seawater sulfate, whereas intermediate, high temperature mineralization was dominated leached, igneous sulphur from basement rocks. These results are similar to modern seafloor vents and many ancient VMS deposits.
Minerals and Enrichment of W, Rb, and Cs in Late Permian Coal from Meitian Mine, Meitian Coalfield, Southern China by Magmatic Hydrothermal Fluids
Authors: Xiao Zhenghui , Cao Yunjiang , Jiang Wei , Zhou Ping , Huang Yanran , Liu Jisong
Published: November 2018

We report on the effects of magmatic hydrothermal fluids on the mineralogical and geochemical compositions of 12U and 12L Coals from the Meitian Mine in the Meitian Coalfield, southern China. The minerals in 12U Coal are predominantly chlorite, quartz, and calcite, while the minerals in 12L Coal consist mainly of illite, quartz, chlorite, kaolinite, and mixed-layer illite/smectite (I/S). The vesicle- and fracture-filling illite, chlorite, I/S, pyrite, and fluorite, cleat- and fracture-filling carbonate minerals (i.e., calcite, and dolomite), and cleat-filling tremolite, diopside, and talc have epigenetic hydrothermal origins. Tremolite, diopside, and talc were probably formed from the reaction between dolomite and Si-rich magmatic hydrothermal fluids. Elevated Pb–Zn–Sn–Cd assemblages are characteristic for the 12U Coal, while 12L Coal is enriched in W, Rb, Cs, Th, V, Zn, and Zr, most notably W, Rb, and Cs. REY (Rare Earth Elements and Yttrium) plots for almost all coals, partings and host rocks are similar, showing an M-type REY distribution, Gd-maximum, positive Y anomalies, and negative Ce anomalies, suggesting acid hydrothermal circulation in the coal-bearing strata. Rubidium and cesium in the coal is clearly associated with K-rich clay minerals (illite + I/S), and to a lesser extent with silicate minerals that were precipitated from hydrothermal solutions. W in the coals mainly occurs in the inorganic constituents of illite and pyrite, especially illite. Enrichment of W, Rb, and Cs in the coal and host rocks is genetically associated with magmatic hydrothermal fluids. Specifically, magmatic hydrothermal fluids of relatively high temperatures that are rich in volatile matter can extract abundant W, Rb, and Cs from granitic melts. The enrichment of these rare metals in the coal is mainly related to illitization. Our study results suggest that, for coal intruded by magmatic rocks, the type of hydrothermal alteration may greatly influence the enrichment of elements.
Plant Tissues and Embryos Biominerals in Sarcocornia pruinosa, a Halophyte from the Río Tinto Salt Marshes
Authors: De la Fuente Vicenta , Rufo Lourdes , Sánchez-Gavilán Irene , Ramírez Esteban , Rodríguez Nuria , Amils Ricardo
Published: November 2018

Although biomineralization in plants is an important area of research, there is very limited information. In this work, we report the location of Na, K, Ca, Mg and Fe biominerals in Sarcocornia pruinosa (Chenopodiacaeae), a halophyte species growing in the estuarine area of Río Tinto, an extreme acidic environment. The estuarine soils of the Tinto basin are characterized by slightly acidic pH and high concentrations of ions. They are exposed to Atlantic Ocean tides that contribute to the increase in pH, Na and Mg concentrations. The aim of this work was to characterize the elemental composition and to identify the biominerals detected in cell tissues of S. pruinosa. Analytical techniques, such as ICP-MS (Inductively coupled plasma mass spectrometry), XRD and microscopy such as OM (optical microscopy) with histochemical staining, SEM and TEM (scanning and transmission electronic microscopy) coupled with EDX (energy dispersive X-ray) were carried out to analyze the plant tissues of S. pruinosa and characterize the detected biominerals. The elemental composition in succulent stems and seeds of S. pruinosa showed high values of Na and K followed by Ca, Mg and Fe. The presence of halite, sylvite, weddellite, glushinskite and Fe oxides biominerals in this halophyte species is reported. Our data suggest the importance of vegetation in the biogeochemical cycles in estuarine areas.
Production of Selenomethionine-Enriched Bifidobacterium bifidum BGN4 via Sodium Selenite Biocatalysis
Authors: Jin Weihong , Yoon Cheolho , Johnston Tony , Ku Seockmo , Ji Geun
Published: November 2018

Selenium is a trace element essential for human health that has received considerable attention due to its nutritional value. Selenium’s bioactivity and toxicity are closely related to its chemical form, and several studies have suggested that the organic form of selenium (i.e., selenomethionine) is more bioavailable and less toxic than its inorganic form (i.e., sodium selenite). Probiotics, especially Bifidobacteriium and Lactobacillus spp., have received increasing attention in recent years, due to their intestinal microbial balancing effects and nutraceutical benefits. Recently, the bioconversion (a.k.a biotransformation) of various bioactive molecules (e.g., minerals, primary and secondary metabolites) using probiotics has been investigated to improve substrate biofunctional properties. However, there have been few reports of inorganic selenium conversion into its organic form using Bifidobacterium and Lactobacillus spp. Here we report that the biosynthesis of organic selenium was accomplished using the whole cell bioconversion of sodium selenite under controlled Bifidobacterium bifidum BGN4 culture conditions. The total amount of organic and inorganic selenium was quantified using an inductively coupled plasma-atomic emission spectrometer (ICP-AES). The selenium species were separated via anion-exchange chromatography and analyzed with inductively coupled plasma-mass spectrometry (ICP-MS). Our findings indicated that the maximum level of organic selenium was 207.5 µg/g in selenium-enriched B. bifidum BGN4. Selenomethionine was the main organic selenium in selenium-enriched B. bifidum BGN4 (169.6 µg/g). Considering that B. bifidum BGN4 is a commercial probiotic strain used in the functional food industry with clinically proven beneficial effects, selenium-enriched B. bifidum BGN4 has the potential to provide dual healthy functions as a daily supplement of selenium and regulator of intestinal bacteria. This is the first report on the production of organic selenium using B. bifidum spp.
Stable Isotope-Labeled Lipidomics to Unravel the Heterogeneous Development Lipotoxicity
Authors: Shih Lu-Min , Tang Hsiang-Yu , Lynn Ke-Shiuan , Huang Cheng-Yu , Ho Hung-Yao , Cheng Mei-Ling
Published: November 2018

Non-alcoholic fatty liver disease (NAFLD) as a global health problem has clinical manifestations ranging from simple non-alcoholic fatty liver (NAFL) to non-alcoholic steatohepatitis (NASH), cirrhosis, and cancer. The role of different types of fatty acids in driving the early progression of NAFL to NASH is not understood. Lipid overload causing lipotoxicity and inflammation has been considered as an essential pathogenic factor. To correlate the lipid profiles with cellular lipotoxicity, we utilized palmitic acid (C16:0)- and especially unprecedented palmitoleic acid (C16:1)-induced lipid overload HepG2 cell models coupled with lipidomic technology involving labeling with stable isotopes. C16:0 induced inflammation and cell death, whereas C16:1 induced significant lipid droplet accumulation. Moreover, inhibition of de novo sphingolipid synthesis by myriocin (Myr) aggravated C16:0 induced lipoapoptosis. Lipid profiles are different in C16:0 and C16:1-treated cells. Stable isotope-labeled lipidomics elucidates the roles of specific fatty acids that affect lipid metabolism and cause lipotoxicity or lipid droplet formation. It indicates that not only saturation or monounsaturation of fatty acids plays a role in hepatic lipotoxicity but also Myr inhibition exasperates lipoapoptosis through ceramide in-direct pathway. Using the techniques presented in this study, we can potentially investigate the mechanism of lipid metabolism and the heterogeneous development of NAFLD.
Frontiers in Computational Chemistry for Drug Discovery
Authors: Luque F. Javier
Published: November 2018

Computational methods pervade almost all aspects of drug discovery [...]
Study of Osteocyte Behavior by High-Resolution Intravital Imaging Following Photo-Induced Ischemia
Authors: Yuan Hengfeng , Jiang Wen , Chen Yuanxin , Kim Betty Y. S.
Published: November 2018

Ischemic injuries and local hypoxia can result in osteocytes dysfunction and play a key role in the pathogenesis of avascular osteonecrosis. Conventional imaging techniques including magnetic resonance imaging (MRI) and computed tomography (CT) can reveal structural and functional changes within bony anatomy; however, characterization of osteocyte behavioral dynamics in the setting of osteonecrosis at the single cell resolution is limited. Here, we demonstrate an optical approach to study real-time osteocyte functions in vivo. Using nicotinamide adenine dinucleotide (NADH) as a biomarker for metabolic dynamics in osteocytes, we showed that NADH level within osteocytes transiently increase significantly after local ischemia through non-invasive photo-induced thrombosis of afferent arterioles followed by a steady decline. Our study presents a non-invasive optical approach to study osteocyte behavior through the modulation of local environmental conditions. Thus it provides a powerful toolkit to study cellular processes involved in bone pathologies in vivo.