bluuebunny/crossref_metadata_2025 huggingface dataset:
The Dimensional Structure of Internalizing Psychopathology: Relation to Diagnostic Categories
Authors: Snyder Hannah R. , Silton Rebecca L. , Hankin Benjamin L. , Smolker Harry R. , Kaiser Roselinde H. , Banich Marie T. , Miller Gregory A. , Heller Wendy
Published: December 2022

Recent approaches have aimed to represent the dimensional structure of psychopathology, but relatively little research has rigorously tested subdimensions within internalizing psychopathology. Using adult samples harmonized across three sites ( N = 427), this study tested preregistered models of the dimensional structure of internalizing psychopathology and their relations with current and lifetime depressive and anxiety disorders diagnostic data. Across S-1 bifactor and hierarchical models, we found converging evidence for both general and specific internalizing dimensions. Depression, generalized anxiety disorder (GAD), social anxiety disorder (SAD), and panic attacks were all associated with a general internalizing factor that we posit primarily represents motivational anhedonia. GAD was also associated with a specific anxious apprehension factor, and SAD was associated with specific anxious apprehension and low positive affect factors. We suggest that dimensional approaches capturing shared and specific internalizing symptom facets more accurately describe the structure of internalizing psychopathology and provide useful alternatives to categorical diagnoses to advance clinical science.
Interaction of zirconium oxide nanoparticle infiltrated resin adhesive with dentin conditioned by phosphoric acid and Er, Cr: YSGG laser
Authors: Aljamhan Abdullah S , Alrefeai Mohammad H , Alhabdan Alhanouf , Alzehiri Mona H , Naseem Mustafa , Vohra Fahim , Alkhudhairy Fahad
Published: December 2022

The aim was to prepare experimental adhesive (EA) with ZrO2 nanoparticles (ZEA) and without it; and its interaction with dentin conditioned with photodynamic therapy (PDT), etch and rinse, and Er, Cr: YSGG laser (ECL). Methods consisted of the scanning electron microscope (SEM), energy dispersive X-ray (EDX), shear bond strength (SBS) failure mode, and rheological property analysis. The methods were employed to assess bond integrity; the shape of ZrO2 NPs and interfacial dentin and adhesive interaction. Ninety premolar teeth were collected and pre-bonding, conditioning of dentin was performed utilizing phosphoric acid (H3PO4), photodynamic therapy (PDT), and ER-CR-YSGG laser (ECL). The teeth were set to form bonded specimens using two adhesives EA and ZEA. The microscopic imagery of ZrO2 revealed irregular-shaped, non-edged particles. EDX analysis revealed Zr to be 75.7% whereas, O2 by weight was 25.3%. The highest SBS was demonstrated in dentin conditioned with etch and rinse and ZEA (19.05 ± 0.13 MPa). Whereas, the lowest SBS was observed in dentin surface treated with PDT and EA (12.25 ± 0.14 MPa). Most of the reported failure modes were adhesive. Rheological analysis revealed that with increasing frequencies the viscosities of adhesives were reduced. The presence of 10% ZrO by weight in adhesive improves SBS of restoration and this improvement in bond values is statistically significant compared to EA. Bond values may be influenced by surface treatment of dentin.
Saving Indian Villages: British Empire, the Great Depression and Gandhi’s Civil Disobedience Movement
Authors: Oak Alok
Published: December 2022

This article traces an intricate relationship between Mahatma Gandhi’s call for Civil Disobedience (1930–1933) and the global economic slump of the 1920s experienced by Britain and colonial India. I argue that the economic hardships faced by Indians (particularly the peasant classes) forced Gandhi to revisit his sociopolitical approach to India’s nationalist movement. Despite the chronological overlap of the Great Depression (1929–1931) with Gandhi’s Civil Disobedience Movement (1930–1933), the relation between these two major events has not been adequately explored in recent scholarship. I propose to contextualize the changes in Gandhi’s economic ideas and political strategy (often against contending ideological trends) leading to his defence of Indian peasant interests during the Gandhi–Irwin Pact and the Second Round Table Conference. Gandhi’s increasing awareness of the economic crises and Britain’s severe opposition to granting financial autonomy to India pushed Gandhi in the direction of charting a new path for economic self-reliance. This, I suggest, resulted in his nation-wide popular movement for reviving the Indian village economy in the form of the ‘Constructive Programme’ (1934–1948) in subsequent years.
Inequality and Capitalism in India
Authors: Bardhan Pranab
Published: December 2022

Capitalism is usually associated with inequality. The Indian variant of capitalism creates its specific structure of inequality. The recent trends of capital concentration in India, facilitated by the political decision-making, shape particular patterns of the rise in inequalities. The nature of capitalism in India is linked to the nature of capital concentration and the low bargaining power of the labour. The article discusses these links. The article discusses the recent attempts at social and political legitimization of the prevailing kind of capitalism under the current political regime. The focus is on long-term issues, and instead of a detailed account, the attempt is to paint a picture with a very broad brush.
Foot and Shank Coordination During Walking in Copers Compared With Patients With Chronic Ankle Instability and Controls
Authors: Kweon Sang Jin , Harrison Kathryn , Williams D.S. Blaise , Kwon Yong Ung
Published: December 2022

Background:

Chronic ankle instability (CAI) alters sensorimotor function and joint coordination, but ankle coordination during walking in copers (patients with a history of ankle sprain without any residual symptoms of CAI) remains unknown.

Purpose:

To identify foot and shank coordination patterns that discriminate among individuals with CAI, copers, and healthy controls and to investigate whether copers display a different strategy to overcome altered sensorimotor function after a lateral ankle sprain compared with individuals with CAI and healthy controls.

Study Design:

Controlled laboratory study.

Methods:

A total of 51 participants (17 participants with CAI, 17 copers, 17 healthy controls) walked on an instrumented treadmill at a fixed speed of 1.20 m/s for a 10-second trial, from which 8 consecutive gait cycles were extracted for analysis. Heel strike and toe-off were identified for each stance phase, and each stance phase was normalized to 100 time frames. A curve analysis was performed to detect group mean differences in vector coding coupling angles and coordination variabilities for sagittal plane ankle motion/transverse plane tibial plane motion (SAK/TT) and frontal plane ankle motion/transverse plane tibial motion (FAK/TT) with 90% CIs.

Results:

During the terminal stance, CAI and coper groups demonstrated an inversion–tibial external rotation coupling, while controls displayed a dorsiflexion–tibial internal rotation strategy. During midstance, there were no differences between the coper, CAI, or control groups. At 0% to 20% of stance, the CAI group showed the most variability, while copers showed the least. During midstance, both copers and controls displayed an increase in variability earlier than the CAI group. The CAI group displayed a peak in variability from 39% to 43% of stance, which was greater than copers. During the propulsive phase (from heel-off to toe-off), the CAI group showed greater SAK/TT variability than both copers and controls. Similar to SAK/TT variability, the CAI group showed an earlier peak in FAK/TT variability compared with controls.

Conclusion:

The CAI, coper, and control groups displayed different ankle joint coupling patterns and coordination variability during a walking gait cycle.

Clinical Relevance:

Copers may have the ability to alter their coordination during walking, which may help us understand the underlying mechanism of CAI.
Echogenic Bowel as an Indicator of Necrotizing Enterocolitis in a Term Newborn
Authors: White Gwenevere , Dulaney Breyanna , Brown Carla , Baber Megan
Published: December 2022

A 3.5-kilogram infant was born at 40 weeks gestation with an uncomplicated delivery. Prenatal ultrasounds showed echogenic bowel and a ventricular septal defect (VSD), of no clinical significance. Abdominal radiographs showed pneumatosis at 21, 36, and 48 hours of life (HOL). She was treated for necrotizing enterocolitis (NEC) with intravenous antibiotics and parenteral nutrition for 7 days, before working up on feeds and discharging home with breast milk. The only prenatal finding in this case was hyperechogenic bowel, which is a soft marker and often disregarded in the absence of other signs. Chronic intrauterine gut ischemia can cause hyperechogenicity of the bowel. That same intrauterine gut ischemia may have been responsible for NEC in our patient. If a patient has persistent echogenic bowel on prenatal imaging, a critical need exists to make sure NEC is not present.
Determination of Key Inflection Points for Maladaptive Responses in US Nurses During the Pandemic: A Network Analysis
Authors: Li Fan , Tokac Umit , Werner Kimberly , Fish Anne F. , Lou Qingqing
Published: December 2022

Introduction

During the pandemic, the nursing workforce is experiencing overwhelming workloads that carry a heavy psychological burden. A wide variety of psychological responses to the COVID-19 pandemic have been studied in nurses globally, but many are not studied or understudied in US nurses. Theoretical underpinnings of the current study are based on the disaster component of the Middle-Range Theory of Nurses' Psychological Trauma.

Objective

To explore the associations of psychological responses (life satisfaction, perceived stress, posttraumatic stress disorder [PTSD] symptomatology, attitude toward life, and compassion satisfaction), years of experience, and general health in US nurses during the COVID-19 pandemic using network analysis.

Methods

A cross-sectional study was conducted using an online survey from October to November 2020 in US nurses. Network analysis was used to model the data and analyze the centrality indices of betweenness, closeness, and strength. Data were reported according to the Strengthening the Reporting of Observational studies in Epidemiology (STROBE) checklist.

Results

In 128 nurses, 19.35% of nurses had probable PTSD. Network analysis showed strong significant correlations between life satisfaction and perceived stress (negative), between perceived stress and PTSD symptomatology (positive), and between attitude toward life and compassion satisfaction (positive).

Conclusion

Low life satisfaction, high perceived stress, and low attitude toward life are key inflection points that signal the need for psychological intervention in the US nursing workforce during the continued pandemic. Based on 2021 Tri-Council of Nursing COVID-19 Report and the 2022 International Council of Nurses guideline, healthcare should implement scalable, system-level interventions to reduce psychological burden during the pandemic. The current study suggests targets for such intervention, which may promote a healthier, more effective US nursing workforce.
Concentration of noxious gases inside and outside residential apartments across different settlements in Port Harcourt metropolis, Nigeria
Authors: Ogaji Folake M , Numbere Aroloye O , Obafemi Andrew , Ogaji Daprim S
Published: December 2022

The proliferation of harmful gases is a major global problem caused by anthropogenic activities. This study hypothesized that the emission of noxious gases varies seasonally in indoor and outdoor environments. Air samples were collected from 40 georeferenced residential areas ( N = 40) in Port Harcourt using Aeroqual 500 monitor to measure six gases: CH4, CO, NH4, NO2, O3, and SO2. Samples were collected at different times, locations, and seasons. The analysis of variance (ANOVA) indicates a significant difference in concentration between gases, times, and locations at p < 0.001. But the interaction effect shows that times and seasons significantly affect noxious gas concentration compared to location. In general, the concentration of CO was the highest, while that of O3 was the lowest. The order of concentration is CO > SO2 > NO2 > CH4 > NH4 > O3. The concentrations of NO2 and SO2 were higher than the WHO limit, while CO, CH4, NH, and O3 were below the WHO limit. Furthermore, CO concentration was highest in the morning, while NO2 and SO2 concentrations were highest in the evening. For seasons, the CH4, NH4, NO2, and SO2 were higher during the dry season, while CO concentration was higher during the wet season. Pollutant levels should be regularly monitored to help provide exposure advisory to city dwellers on the impact of atmospheric pollution on their health.
Restoration of Anatomic Parameters and Syndesmotic Reduction After Intramedullary Nailing of Distal Fibular Fractures
Authors: Bastías Gonzalo F. , Bravo Francisco , Astudillo Claudia , Giannini Esteban , Contreras Martin , Melo Rodrigo , Muñoz Gerardo , Pellegrini Manuel J. , Cuchacovich Natalio
Published: December 2022

Background:

Intramedullary nailing of the fibula (FN) is a method of fixation that has proven to be useful for treating distal fibular fractures (DFs). FN minimizes soft tissue complications and provides similar stability to plating, with fewer hardware-related symptoms. Nevertheless, FN has been associated with syndesmotic malreduction and the incapacity of restoring length and rotation of the fibula. We aimed to evaluate the fibular position and syndesmotic reduction after fixation with FN compared with the uninjured ankle in the immediate postoperative period.

Methods:

Prospective cohort study. Patients with DF fractures treated with IN between January 2017 and January 2020 were included. Immediate postoperative bilateral ankle CT was obtained in all cases. Fibular rotation, length, and translation as well as syndesmotic diastasis were measured on both ankles and compared by 3 independent observers.

Results:

Twenty-eight patients were included (16 women). The mean age was 46 years (range 16-91). Fracture type distribution according to AO/ASIF classification included 19 patients with 44.B (67.9%), 8 patients with 44.C (28.6%), and 1 patient with a 44.A fracture (3.6%). No significant differences were identified considering fibular rotation ( P = .661), syndesmotic diastasis ( P = .147), and fibular length ( P = .115) between the injured and uninjured ankle. Fibular translation had statistical differences ( P = .01) compared with the uninjured ankle. The intraclass correlation coefficient showed an excellent concordance between observers except for fibular translation on the injured ankle.

Conclusion:

In this cohort, fixation of DF fractures with FN allows restoration of anatomical parameters of the ankle in terms of fibular rotation, length, and syndesmotic diastasis. However, fibular translation had significant differences compared with the uninjured ankle based on bilateral CT scan evaluation.

Level of Evidence:

Level II, prospective cohort study.
Prediction of impact behaviour for natural fiber-reinforced composites using the finite element method
Authors: Prasanthi Parvathaneni Phani , Madhav Vallabhaneni Venkata Venu , Chaitanya Chadalavada Sri , Spandana Vallabhaneni Veda , Saxena Kuldeep K , Garg Sahil , Zeleke Migbar A
Published: December 2022

In the past ten years, as awareness of biodegradability has increased, so has the utilization of natural fiber-reinforced composites. Along with the material properties, dynamic responsiveness is also necessary for the efficient design of these natural reinforced composites. In the current work, elastic characteristics and interfacial stress are evaluated for natural fiber-reinforced composites utilizing micromechanics and finite element methods. Later, employing explicit dynamic analysis, the natural composite plate was examined under impact loading. The analytical results used to verify the finite element models at each stage show good agreement. To carry out the current study, natural fiber-reinforced composites like hemp, sisal and flax as well as hemp + sisal, sisal + flax and hemp + flax hybrid composites were evaluated for their elastic modulus in longitudinal, transverse, in-plane and out of plane directions as well as their major and minor Poisson’s ratio. By adjusting the impactor’s velocity from 2 m/s to 11 m/s, the deformation, stresses, internal energy and energy summary of the hybrid natural fiber-reinforced composite are calculated from the impact analysis. Based on all the findings, the performance of hemp fiber and hemp fiber-based hybrid composites is better than all other composites taken into consideration for the current work. This research is utilized to build composite materials that function effectively under gradual loading.
Cerebral perfusion variance in new daily persistent headache and chronic migraine: an arterial spin-labeled MR imaging study
Authors: Bai Xiaoyan , Wang Wei , Zhang Xueyan , Hu Zhangxuan , Zhang Yingkui , Li Zhiye , Zhang Xue , Yuan Ziyu , Tang Hefei , Zhang Yaqing , Yu Xueying , Zhang Peng , Wang Yonggang , Sui Binbin
Published: December 2022

Background and purpose

New daily persistent headache (NDPH) and chronic migraine (CM) are two different types of headaches that might involve vascular dysregulation. There is still a lack of clarity about altered brain perfusion in NDPH and CM. This study aimed to investigate the cerebral perfusion variances of NDPH and CM using multi-delay pseudo-continuous arterial spin-labeled magnetic resonance imaging (pCASL-MRI).

Methods

Fifteen patients with NDPH, 18 patients with CM, and 15 age- and sex-matched healthy controls (HCs) were included. All participants underwent 3D multi-delay pCASL-MRI to obtain cerebral perfusion data, including arrival-time-corrected cerebral blood flow (CBF) and arterial cerebral blood volume (aCBV). The automated anatomical labeling atlas 3 (AAL3) was used to parcellate 170 brain regions. The CBF and aCBV values in each brain region were compared among the three groups. Correlation analyses between cerebral perfusion parameters and clinical variables were performed.

Results

Compared with HC participants, patients with NDPH were found to have decreased CBF and aCBV values in multiple regions in the right hemisphere, including the right posterior orbital gyrus (OFCpost.R), right middle occipital gyrus (MOG.R), and ventral anterior nucleus of right thalamus (tVA.R), while patients with CM showed increased CBF and aCBV values presenting in the ventral lateral nucleus of left thalamus (tVL.L) and right thalamus (tVL.R) compared with HCs (all p < 0.05). In patients with NDPH, after age and sex adjustment, the increased aCBV values of IFGorb. R were positively correlated with GAD-7 scores; and the increased CBF and aCBV values of tVA.R were positively correlated with disease duration.

Conclusion

The multi-delay pCASL technique can detect cerebral perfusion variation in patients with NDPH and CM. The cerebral perfusion changes may suggest different variations between NDPH and CM, which might provide hemodynamic evidence of these two types of primary headaches.
ReCSAI: recursive compressed sensing artificial intelligence for confocal lifetime localization microscopy
Authors: Reinhard Sebastian , Helmerich Dominic A. , Boras Dominik , Sauer Markus , Kollmannsberger Philip
Published: December 2022

Background

Localization-based super-resolution microscopy resolves macromolecular structures down to a few nanometers by computationally reconstructing fluorescent emitter coordinates from diffraction-limited spots. The most commonly used algorithms are based on fitting parametric models of the point spread function (PSF) to a measured photon distribution. These algorithms make assumptions about the symmetry of the PSF and thus, do not work well with irregular, non-linear PSFs that occur for example in confocal lifetime imaging, where a laser is scanned across the sample. An alternative method for reconstructing sparse emitter sets from noisy, diffraction-limited images is compressed sensing, but due to its high computational cost it has not yet been widely adopted. Deep neural network fitters have recently emerged as a new competitive method for localization microscopy. They can learn to fit arbitrary PSFs, but require extensive simulated training data and do not generalize well. A method to efficiently fit the irregular PSFs from confocal lifetime localization microscopy combining the advantages of deep learning and compressed sensing would greatly improve the acquisition speed and throughput of this method.

Results

Here we introduce ReCSAI, a compressed sensing neural network to reconstruct localizations for confocal dSTORM, together with a simulation tool to generate training data. We implemented and compared different artificial network architectures, aiming to combine the advantages of compressed sensing and deep learning. We found that a U-Net with a recursive structure inspired by iterative compressed sensing showed the best results on realistic simulated datasets with noise, as well as on real experimentally measured confocal lifetime scanning data. Adding a trainable wavelet denoising layer as prior step further improved the reconstruction quality.

Conclusions

Our deep learning approach can reach a similar reconstruction accuracy for confocal dSTORM as frame binning with traditional fitting without requiring the acquisition of multiple frames. In addition, our work offers generic insights on the reconstruction of sparse measurements from noisy experimental data by combining compressed sensing and deep learning. We provide the trained networks, the code for network training and inference as well as the simulation tool as python code and Jupyter notebooks for easy reproducibility.
An unbiased kinship estimation method for genetic data analysis
Authors: Jiang Wei , Zhang Xiangyu , Li Siting , Song Shuang , Zhao Hongyu
Published: December 2022

Accurate estimate of relatedness is important for genetic data analyses, such as heritability estimation and association mapping based on data collected from genome-wide association studies. Inaccurate relatedness estimates may lead to biased heritability estimations and spurious associations. Individual-level genotype data are often used to estimate kinship coefficient between individuals. The commonly used sample correlation-based genomic relationship matrix (scGRM) method estimates kinship coefficient by calculating the average sample correlation coefficient among all single nucleotide polymorphisms (SNPs), where the observed allele frequencies are used to calculate both the expectations and variances of genotypes. Although this method is widely used, a substantial proportion of estimated kinship coefficients are negative, which are difficult to interpret. In this paper, through mathematical derivation, we show that there indeed exists bias in the estimated kinship coefficient using the scGRM method when the observed allele frequencies are regarded as true frequencies. This leads to negative bias for the average estimate of kinship among all individuals, which explains the estimated negative kinship coefficients. Based on this observation, we propose an unbiased estimation method, UKin, which can reduce kinship estimation bias. We justify our improved method with rigorous mathematical proof. We have conducted simulations as well as two real data analyses to compare UKin with scGRM and three other kinship estimating methods: rGRM, tsGRM, and KING. Our results demonstrate that both bias and root mean square error in kinship coefficient estimation could be reduced by using UKin. We further investigated the performance of UKin, KING, and three GRM-based methods in calculating the SNP-based heritability, and show that UKin can improve estimation accuracy for heritability regardless of the scale of SNP panel.
EndHiC: assemble large contigs into chromosome-level scaffolds using the Hi-C links from contig ends
Authors: Wang Sen , Wang Hengchao , Jiang Fan , Wang Anqi , Liu Hangwei , Zhao Hanbo , Yang Boyuan , Xu Dong , Zhang Yan , Fan Wei
Published: December 2022

Background

The application of PacBio HiFi and ultra-long ONT reads have enabled huge progress in the contig-level assembly, but it is still challenging to assemble large contigs into chromosomes with available Hi-C scaffolding tools, which count Hi-C links between contigs using the whole or a large part of contig regions. As the Hi-C links of two adjacent contigs concentrate only at the neighbor ends of the contigs, larger contig size will reduce the power to differentiate adjacent (signal) and non-adjacent (noise) contig linkages, leading to a higher rate of mis-assembly.

Results

We design and develop a novel Hi-C based scaffolding tool EndHiC, which is suitable to assemble large contigs into chromosomal-level scaffolds. The core idea behind EndHiC, which distinguishes it from other Hi-C scaffolding tools, is using Hi-C links only from the most effective regions of contig ends. By this way, the signal neighbor contig linkages and noise non-neighbor contig linkages are separated more clearly. Benefiting from the increased signal to noise ratio, the reciprocal best requirement, as well as the robustness evaluation, EndHiC achieves higher accuracy for scaffolding large contigs compared to existing tools. EndHiC has been successfully applied in the Hi-C scaffolding of simulated data from human, rice and Arabidopsis, and real data from human, great burdock, water spinach, chicory, endive, yacon, and Ipomoea cairica, suggesting that EndHiC can be applied to a broad range of plant and animal genomes.

Conclusions

EndHiC is a novel Hi-C scaffolding tool, which is suitable for scaffolding of contig assemblies with contig N50 size near or over 10 Mb and N90 size near or over 1 Mb. EndHiC is efficient both in time and memory, and it is interface-friendly to the users. As more genome projects have been launched and the contig continuity constantly improved, we believe EndHiC has the potential to make a great contribution to the genomics field and liberate the scientists from labor-intensive manual curation works.
Construction of transcriptome atlas of white yak hair follicle during anagen and catagen using single-cell RNA sequencing
Authors: Zheng Qingbo , Ye Na , Bao Pengjia , Zhang Xiaolan , Wang Fubin , Ma Lanhua , Chu Min , Guo Xian , Liang Chunnian , Pan Heping , Yan Ping
Published: December 2022

Background

As the direct organ of villus, hair follicles have obvious seasonal cycles. The hair follicle cycle is orchestrated by multiple cell types that together direct cell renewal and differentiation. But the regulation property of hair follicle cells from anagen to catagen in yak is still unknown.

Results

In this study, single-cell RNA sequencing was performed on 24,124 single cells of the scapular skin from white yak. Based on tSNE cluster analysis, the cell types of IFE-DC, epidermal cell lines, fibroblasts, keratinocytes, IRS, DS, INFU, and other cells in yak hair follicles during anagen and catagen were successfully identified, and the gene expression profiles were described. The GO enrichment analysis indicated the different cells characteristic genes to be mainly enriched in the epidermal development, epithelial cell differentiation and wound healing pathways. The pseudotime trajectory analysis described the differentiation trajectory of the epidermal lineage and dermal lineage of the hair follicle during anagen and catagen. Moreover, the dynamic changes of the genes like LHX2, KRT25, and KRT71 were found to be highly expressed in HS and IRS, but not in the IFE-DC, INFU, and keratinocyte during differentiation.

Conclusions

Our results analyzed the time-varying process of gene expression in the dermal cell lineage and epidermal cell lineage of hair follicles during anagen and catagen during fate differentiation was expounded at the single cell level, revealing the law of fate specialization of different types of cells. In addition, based on the enrichment analysis, the transcriptional regulatory factors involved in the different cell fates were also revealed. These results will help to enhance our understanding of yak hair follicle cycle and promote the development and utilization of yak villus.
A comparative transcriptomic analysis reveals a coordinated mechanism activated in response to cold acclimation in common vetch (Vicia sativa L.)
Authors: Dong Rui , Luo Ben , Tang Li , Wang Qiu-xia , Lu Zhong-Jie , Chen Chao , Yang Feng , Wang Song , He Jin
Published: December 2022

Background

Due to its strong abiotic stress tolerance, common vetch is widely cultivated as a green manure and forage crop in grass and crop rotation systems. The comprehensive molecular mechanisms activated in common vetch during cold adaptation remain unknown.

Results

We investigated physiological responses and transcriptome profiles of cold-sensitive (Lanjian No. 1) and cold-tolerant (Lanjian No. 3) cultivars during cold acclimation to explore the molecular mechanisms of cold acclimation. In total, 2681 and 2352 differentially expressed genes (DEGs) were identified in Lanjian No. 1 and Lanjian No. 3, respectively; 7532 DEGs were identified in both lines. DEGs involved in “plant hormone signal transduction” were significantly enriched during cold treatment, and 115 DEGs involved in cold-processed hormone signal transduction were identified. Common vetch increased the level of indoleacetic acid (IAA) by upregulating the transcriptional regulator Aux/IAA and downregulating GH3, endowing it with stronger cold tolerance. An auxin-related DEG was overexpressed in yeast and shown to possess a biological function conferring cold tolerance.

Conclusion

This study identifies specific genes involved in Ca2+ signaling, redox regulation, circadian clock, plant hormones, and transcription factors whose transcriptional differentiation during cold acclimation may improve cold tolerance and contributes to the understanding of common and unique molecular mechanisms of cold acclimation in common vetch. The candidate genes identified here also provide valuable resources for further functional genomic and breeding studies.
Midterm outcomes of simultaneous carotid revascularization combined with coronary artery bypass grafting
Authors: Jia Songhao , Wang Maozhou , Gong Ming , Zhang Hongjia , Jiang Wenjian
Published: December 2022

Background

Simultaneous carotid endarterectomy (CEA) combined with coronary artery bypass grafting (CABG) has been widely used in patients with coronary heart disease complicated with severe carotid stenosis to reduce the risk of stroke and death. Carotid artery stenting (CAS) has been proven to be an alternative to CEA in recent years. We investigated the early and mid-term outcomes of simultaneous CEA or CAS combined with CABG in these patients.

Methods

From January 2011 to January 2021, 88 patients who underwent simultaneous carotid revascularization combined with CABG under the same anesthesia in Beijing Anzhen Hospital were retrospectively analyzed, and this study included 25 patients who underwent CAS–CABG and 63 patients who underwent CEA–CABG. The main outcomes included all-cause death, stroke, myocardial infarction and combined adverse events. The main outcomes of the two groups were compared at 30 days after the operation and the mid-term follow-up. Univariate and multivariate Cox proportional hazards regression analyses were performed to determine the independent risk factors affecting mid-term mortality.

Results

Within 30 days after the operation, there was no significant difference in combined adverse events between the two groups (P = 0.88). During the median follow-up period of 6.69 years (IQR, 5.82–7.57 years), 9 patients (14.30%) in the combined CEA–CABG group died, while 1 patient (4.00%) in the combined CAS–CABG group died. There were no significant differences in mid-term death (P = 0.20), stroke (P = 0.78), myocardial infarction (P = 0.88), or combined adverse events (P = 0.62) between the two groups. Univariate and multivariate Cox proportional hazards regression showed that NYHA grade IV (HR 5.01, 95% CI 1.16–21.64, P = 0.03) and previous myocardial infarction (HR 5.43, 95% CI 1.01–29.29, p = 0.04) were independent risk factors for mid-term mortality. We also found that combined CEA–CABG surgery may be associated with a higher risk of death (HR, 13.15; 95% CI 1.10–157.69, p = 0.04).

Conclusions

Combined CAS–CABG is a safe and effective treatment for patients with coronary heart disease complicated with severe carotid stenosis. NYHA grade IV and previous MI were independent risk factors for mid-term mortality.
The mediating role of psychological resilience between social participation and life satisfaction among older adults in China
Authors: Liao Zhiliu , Zhou Hanmeng , He Zhifei
Published: December 2022

Background

A significant correlation has been discovered between social participation and older adults’ life satisfaction, but the relationships among social participation, psychological resilience, and life satisfaction remain to be confirmed. Therefore, this study aims to identify the relationship between social participation and life satisfaction for Chinese older adults and to analyse the possible mediating role of psychological resilience between these two aspects.

Methods

Data on 15,779 people aged 65 years and above were extracted from the 2018 wave of the Chinese Longitudinal Healthy Longevity Survey (CLHLS). Social participation was classified into two levels: low-level involvement activities and high-level involvement activities. Hierarchical regression analysis was applied to analyse the correlations between the two levels of social participation and older adults’ life satisfaction as well as the mediating effects of psychological resilience on this association.

Results

The results indicate that two levels of social participation were each positively correlated with life satisfaction. Specifically, high-level involvement activities (β = 0.070, P < 0.001) were more strongly associated with life satisfaction than low-level involvement activities (β = 0.051, P < 0.001). Moreover, psychological resilience was found to partially mediate the association between low-level involvement activities and high-level involvement activities and life satisfaction.

Conclusion

A higher level of life satisfaction for older adults is related to participation in high-level involvement activities. Psychological resilience has a mediating effect on the association between two levels of older adults’ social participation and life satisfaction. These findings suggest that the government and society should establish a more concrete understanding of the psychological resilience of older adults.
Presence of gastrointestinal symptoms in IgA nephropathy: a cross-sectional study
Authors: Pohjonen Jussi T. , Kaukinen Katri M. , Metso Martti J. , Nurmi Rakel KK. , Huhtala Heini SA. , Pörsti Ilkka H. , Mustonen Jukka T. , Mäkelä Satu M.
Published: December 2022

Background

Gastrointestinal (GI) symptoms are common in end-stage kidney disease. Mounting evidence indicates that the intestine plays an important role in the pathogenesis of IgA nephropathy (IgAN). However, no studies have addressed the obvious question; do IgAN patients suffer from GI symptoms?

Methods

Presence of GI symptoms and health-related quality of life were evaluated using the validated Gastrointestinal Symptom Rating Scale (GSRS) and Psychological General Well-Being (PGWB) questionnaires in 104 patients with kidney biopsy-verified IgAN and in 147 healthy controls. A person was regarded to experience ‘increased GI symptoms’ if the GSRS score exceeded plus 1 standard deviation of the mean of the corresponding score in the healthy controls.

Results

According to the GSRS total score, the IgAN patients had more GI symptoms than the healthy controls (2.0 vs. 1.7, p < 0.001). Female IgAN patients had higher GSRS total score than male patients (2.2 vs. 1.7, p = 0.001). More IgAN patients with preserved kidney function (eGFR > 60ml/min/1.73m2) suffered from increased symptoms of diarrhoea (76 vs. 25%, p = 0.028), constipation (81 vs. 19%, p = 0.046) and reflux (85 vs. 15%, p = 0.004) than did IgAN patients with reduced kidney function (eGFR < 60ml/min/1.73m2).

Conclusions

IgAN patients and especially female IgAN patients experienced more GI symptoms than healthy controls. More prevalent GI symptoms were already observed before kidney function was clearly reduced. Systematic enquiry of GI symptoms might increase the standard of care among IgAN patients. Moreover, GI symptoms may provide clues for future studies that examine the pathophysiology of IgAN.
Prognostic value of the combination of neutrophil-to-lymphocyte ratio, monocyte-to-lymphocyte ratio and platelet-to-lymphocyte ratio on mortality in patients on maintenance hemodialysis
Authors: Liao Jiaxian , Wei Dongyan , Sun Chenghui , Yang Yuqi , Wei Yinxia , Liu Xinhui
Published: December 2022

Background

Hemodialysis (HD) is the most important renal replacement therapy for patients with end-stage kidney disease (ESKD). Systemic inflammation is a risk factor of mortality in HD patients. Neutrophil-to-lymphocyte ratio (NLR), monocyte-to-lymphocyte ratio (MLR), and platelet-to-lymphocyte ratio (PLR) are new inflammatory markers. However, previous studies have inconsistent conclusions about the predictive value of NLR, MLR and PLR on mortality of HD patients. The aim of this study was to establish an inflammation scoring system by including NLR, MLR and PLR, and evaluate the association between the inflammation score and all-cause and cardiovascular mortality in HD patients.

Methods

In this single center retrospective cohort study, 213 incident HD patients from January 1, 2015 to December 31, 2020 were included. Baseline demographic and clinical data and laboratory measurements were collected. According to the optimal cut-off values, NLR, MLR and PLR were assigned 0 or 1 point, respectively. Then, the inflammation score was obtained by adding the NLR, MLR and PLR scores. All patients were followed until July 31, 2021. The associations of the inflammation score with all-cause and cardiovascular mortality were assessed by multivariable-adjusted Cox models.

Results

Of 213 patients, the mean (± SD) age was 56.8 ± 14.4 years, 66.2% were men, and 32.9% with diabetes. The primary cause of ESKD was mainly chronic glomerulonephritis (46.5%) and diabetic nephropathy (28.6%). The median inflammation score was 2 (interquartile range = 1–3). During a median 30 months (interquartile range = 17–50 months) follow-up period, 53 patients had died, of which 33 deaths were caused by cardiovascular disease. After adjusting for demographics, primary diseases and other confounders in multivariable model, the inflammation score = 3 was associated with a hazard ratio for all-cause mortality of 4.562 (95% confidence interval, 1.342–15.504, P = 0.015) and a hazard ratio for cardiovascular mortality of 4.027 (95% confidence interval, 0.882–18.384, P = 0.072).

Conclusion

In conclusion, an inflammation scoring system was established by including NLR, MLR and PLR, and the higher inflammation score was independently associated with all-cause mortality in HD patients.
The impact of prolonged landscape fire smoke exposure on women with asthma in Australia
Authors: Beyene Tesfalidet , Murphy Vanessa E. , Gibson Peter G. , McDonald Vanessa M. , Van Buskirk Joe , Holliday Elizabeth G. , Vertigan Anne E. , Horvat Jay C. , Zosky Graeme R. , Morgan Geoffrey G. , Jegasothy Edward , Hanigan Ivan , Mattes Joerg , Collison Adam M. , Jensen Megan E.
Published: December 2022

Background

Little is known about the physical and mental health impact of exposure to landscape fire smoke in women with asthma. This study examined the health impacts and information-seeking behaviours of women with asthma exposed to the 2019/2020 Australian fires, including women who were pregnant.

Methods

Women with asthma were recruited from the Breathing for Life Trial in Australia. Following the landscape fire exposure period, self-reported data were collected regarding symptoms (respiratory and non-respiratory), asthma exacerbations, wellbeing, quality of life, information seeking, and landscape fire smoke exposure mitigation strategies. Participants’ primary residential location and fixed site monitoring was used to geolocate and estimate exposure to landscape fire-related fine Particulate Matter (PM2.5).

Results

The survey was completed by 81 pregnant, 70 breastfeeding and 232 non-pregnant and non-breastfeeding women with asthma. Participants had a median daily average of 17 μg/m3 PM2.5 and 105 μg/m3 peak PM2.5 exposure over the fire period (October 2019 to February 2020). Over 80% of participants reported non-respiratory and respiratory symptoms during the fire period and 41% reported persistent symptoms. Over 82% reported asthma symptoms and exacerbations of asthma during the fire period. Half the participants sought advice from a health professional for their symptoms. Most (97%) kept windows/doors shut when inside and 94% stayed indoors to minimise exposure to landscape fire smoke. Over two in five (43%) participants reported that their capacity to participate in usual activities was reduced due to prolonged smoke exposure during the fire period. Participants reported greater anxiety during the fire period than after the fire period (mean (SD) = 53(13) versus 39 (13); p < 0.001). Two in five (38%) pregnant participants reported having concerns about the effect of fire events on their pregnancy.

Conclusion

Prolonged landscape fire smoke exposure during the 2019/2020 Australian fire period had a significant impact on the health and wellbeing of women with asthma, including pregnant women with asthma. This was despite most women taking actions to minimise exposure to landscape fire smoke. Effective and consistent public health messaging is needed during landscape fire events to guard the health of women with asthma.
Recurrent miscarriage in a woman with congenital factor V deficiency: a case report
Authors: Shirzadi Mohammad , Radfar Amir Hossein , Dehghani Mehdi
Published: December 2022

Background

Factor V deficiency is a rare bleeding disorder that can be either congenital or acquired. Factor V deficiency mostly present with mucosal bleeding. Coagulation factor V does not increase considerably during normal gestation. Since pregnancy can be threatened by blood clotting disorders, abnormal changes in coagulation factors level can pose challenges to pregnant women.

Case presentation

We report a 40-year-old pregnant woman with prolonged gingival bleeding and epistaxis at 28 weeks of pregnancy. Her past medical history included two unexplained abortions. Physical examination was unremarkable, but the blood test showed elevated PT and PTT with a considerable decrease in factor V activity, while other factors were within normal range. Subsequently, the patient was diagnosed with congenital factor V deficiency. After treatment with fresh frozen plasma, she underwent vaginal delivery and a baby with factor V deficiency was born.

Conclusions

This is the second report of recurrent miscarriage in congenital factor V deficiency patients. Clinicians should consider the possibility of factor V deficiency in women with a history of idiopathic miscarriage even in patients without any symptoms.
Reply to: ‘comments on ‘effects of refractive accommodation on subfoveal choroidal thickness in silicone oil-filled eyes”
Authors: Yan Ying , Zeng Bo , Gao Chengyuan , Song Yanping , Chen Xiao
Published: December 2022

In this article, we answered the questions in Lei Gao et al.’s comments on the "effects of refractive accommodation on subfoveal choroidal thickness in silicone oil-filled eyes" one by one.
Annular choroidal detachment following intravitreal aflibercept injection in a patient with nivolumab treatment: a case report
Authors: Sato Maho , Kubono Hirohisa , Yamashita Kazuya , Nagamoto Takashi , Ofuji Yoshiko , Sakakura Saki , Fukumoto Ryuki , Hata Seiichiro , Kawamura Mari , Suzuki Kotaro
Published: December 2022

Background

To present a novel case that developed annular choroidal detachment after intravitreal anti-vascular endothelial growth factor antibody injection in a patient after immune checkpoint inhibitor treatment.

Case presentation

A 58-year-old Japanese man presented visual impairment in the right eye. Ophthalmological examination revealed macular edema in the right eye, which suggested the possibility of age-related macular degeneration. Following the intravitreal aflibercept injection, the annular choroidal detachment was observed in the injected eye. As hypotony or thick sclera was not observed, choroidal detachment seemed to have appeared due to enhanced inflammation by intravitreal injection. The patient had a history of stage IV paranasal cavity cancer and was treated with nivolumab, an immune checkpoint inhibitor. The immune response might have been enhanced due to the use of nivolumab so that intravitreal injection triggered inflammation. Three weeks after sub-tenon injection of triamcinolone acetonide, macular edema and choroidal detachment improved.

Conclusions

Intravitreal aflibercept injection caused annular choroidal detachment in our patient, presumably because the immune system was activated after nivolumab treatment. To the best of our knowledge, this is the first case report of annular choroidal detachment that developed after intravitreal injection in a patient with a history of nivolumab therapy. With the increasing use of immune checkpoint inhibitors in patients with various cancers, clinicians should be aware of these potentially associated immune-related adverse events.
Role of corneal epithelial thickness during myopic regression in femtosecond laser-assisted in situ keratomileusis and transepithelial photorefractive keratectomy
Authors: Li Hua , Han Qichao , Zhang Jiafan , Shao Ting , Wang Huifeng , Long Keli
Published: December 2022

Background

The study aimed to investigate the relationship between changes in corneal epithelial thickness and the outcome of myopic regression after femtosecond laser-assisted in situ keratomileusis (FS-LASIK) and transepithelial photorefractive keratectomy (TPRK).

Methods

This study included 45 eyes of 25 patients undergoing FS-LASIK and 44 eyes of 24 patients undergoing TPRK. Myopic regression occurred in these patients postoperatively from 8 to 21 months. The corneal epithelial thickness was measured using a spectral-domain optical coherence tomography at the onset of regression, 3 months after treatment, and 3 months after drug withdrawal.

Results

Compared with that of preoperation, corneal epithelial thickness increased when regression occurred in both groups (all P < 0.05). The thickness of central corneal epithelium in FS-LASIK and TPRK groups reached 65.02 ± 4.12 µm and 61.63 ± 2.91 µm, respectively. The corneal epithelial thickness decreased when myopic regression subsided after 3 months of steroid treatment compared to the onset (P < 0.05). With a decrease in corneal epithelial thickness, the curvature of the anterior corneal surface, central corneal thickness, and refractive power all decreased (all P < 0.05). The corneal epithelial thickness and refractive error remained relatively stable after 3 months of treatment withdrawal (P > 0.05).

Conclusion

The corneal epithelial thickness determined the outcome of myopic regression similarly in FS-LASIK and TPRK. When the corneal epithelium thickened, regression occurred. After steroid treatment, epithelial thickness decreased whereas regression subsided.