bluuebunny/crossref_metadata_2025 huggingface dataset:
Conceptual Design of a Comprehensive Farm Nitrogen Management System
Authors: Weckesser Fabian , Leßke Frank , Luthardt Marco , Hülsbergen Kurt-Jürgen
Published: December 2021

Data that are required for nutrient management are becoming increasingly available in digital format, leading to a high innovation potential for digital nitrogen (N) management applications. However, it is currently difficult for farmers to analyze, assess, and optimize N flows in their farms using the existing software. To improve digital N management, this study identified, evaluated, and systematized the requirements of stakeholders. Furthermore, digital farm N management tools with varying objectives in terms of system boundaries, data requirements, used methods and algorithms, performance, and practicality were appraised and categorized. According to the identified needs, the concept of a farm N management system (FNMS) software is presented which includes the following modules: (1) management of site and farm data, (2) determination of fertilizer requirements, (3) N balancing and cycles, (4) N turnover and losses, and (5) decision support. The aim of FNMS is to support farmers in their farming practices for increasing N efficiency and reducing environmentally harmful N surpluses. In this study, the conceptual requirements from the agricultural and computer science perspectives were determined as a basis for developing a consistent, scientifically sound, and user-friendly FNMS, especially applicable in European countries. This FNMS enables farmers and their advisors to make knowledge-based decisions based on comprehensive and integrated data.
A Preliminary Investigation of Interspecific Chemosensory Communication of Emotions: Can Humans (Homo sapiens) Recognise Fear- and Non-Fear Body Odour from Horses (Equus ferus caballus)
Authors: Sabiniewicz Agnieszka , Białek Michał , Tarnowska Karolina , Świątek Robert , Dobrowolska Małgorzata , Sorokowski Piotr
Published: December 2021

Mammalian body odour conveys cues about an individual’s emotional state that can be recognised by conspecifics. Thus far, little attention has been paid to interspecific odour communication of emotions, and no studies have examined whether humans are able to recognise animal emotions from body odour. Thus, the aim of the present study was to address this question. Body odour samples were collected from 16 two-year-old thoroughbred horses in fear and non-fear situations, respectively. The horse odour samples were then assessed by 73 human odour raters. We found that humans, as a group, were able to correctly assign whether horse odour samples were collected under a fear- or a non-fear condition, respectively. Furthermore, they perceived the body odour of horses collected under the fear condition as more intense, compared with the non-fear condition. An open question remains, which is whether humans could simply distinguish between little versus much sweat and between high intensity versus low intensity or were able to recognise horses’ fear and non-fear emotions. These results appear to fit the notion that the ability to recognise emotions in other species may present an advantage to both the sender and the receiver of emotional cues, particularly in the interaction between humans and domesticated animals. To conclude, the present results indicate that olfaction might contribute to the human recognition of horse emotions. However, these results should be addressed with caution in light of the study’s limitations and only viewed as exploratory for future studies.
Myocardial Injury Complicated by Systolic Dysfunction in a COVID-19-Positive Dog
Authors: Romito Giovanni , Bertaglia Teresa , Bertaglia Luigi , Decaro Nicola , Uva Annamaria , Rugna Gianluca , Moreno Ana , Vincifori Giacomo , Dondi Francesco , Diana Alessia , Cipone Mario
Published: December 2021

A six-year-old Cavalier King Charles spaniel was referred with a two-month history of severe exercise intolerance and syncope. Clinical signs had developed during a local wave of coronavirus disease (COVID-19) two weeks after its family members had manifested symptoms of this viral disease and their positivity to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was confirmed. Cardiologic assessment documented myocardial injury complicated by systolic dysfunction. An extensive diagnostic work-up allowed us to rule out common causes of myocardial compromise, both infective and not. Accordingly, serological and molecular tests aimed at diagnosing SARS-CoV-2 infection were subsequently performed, especially in light of the dog’s peculiar history. Results of such tests, interpreted in the light of previous findings and current knowledge from human medicine, supported a presumptive diagnosis of COVID-19-associated myocardial injury, a clinical entity hitherto poorly described in this species.
Susceptibility and Pathology in Juvenile Atlantic Cod Gadus morhua to a Marine Viral Haemorrhagic Septicaemia Virus Isolated from Diseased Rainbow Trout Oncorhynchus mykiss
Authors: Sandlund Nina , Johansen Renate , Fiksdal Ingrid U. , Einen Ann Cathrine B. , Modahl Ingebjørg , Gjerset Britt , Bergh Øivind
Published: December 2021

The first known outbreak caused by a viral haemorrhagic septicaemia virus (VHSV) strain of genotype III in rainbow trout occurred in 2007 at a marine farm in Storfjorden, Norway. The source of the virus is unknown, and cod and other marine fish around the farms are suspected as a possible reservoir. The main objective of this study was to test the susceptibility of juvenile Atlantic cod to the VHSV isolate from Storfjorden. As the pathology of VHS in cod is sparsely described, an additional aim of the study was to give a histopathological description of the disease. Two separate challenge experiments were carried out, using both intra peritoneal (ip) injection and cohabitation as challenge methods. Mortality in the ip injection experiment leveled at approximately 50% three weeks post challenge. Both immunohistochemical and rRT-PCR analysis of organs sampled from diseased and surviving fish confirmed VHSV infection. No VHSV was detected in the cohabitants. The results indicate that Atlantic cod has a low natural susceptibility to this VHSV genotype III strain. One of the most extensive pathological changes was degeneration of cardiac myocytes. Immunohistochemistry confirmed that the lesions were related to VHSV. In some fish, the hematopoietic tissue of spleen and kidney showed degeneration and immunostaining, classical signs of VHS, as described in rainbow trout. Positive immunostaining of the capillaries of the gills, suggests this organ as a useful alternative when screening for VHSV.
Correction: Gulakhmadov et al. Evaluation of the CRU TS3.1, APHRODITE_V1101, and CFSR Datasets in Assessing Water Balance Components in the Upper Vakhsh River Basin in Central Asia. Atmosphere 2021, 12, 1334
Authors: Gulakhmadov Aminjon , Chen Xi , Gulakhmadov Manuchekhr , Kobuliev Zainalobudin , Gulahmadov Nekruz , Peng Jiabin , Li Zhengyang , Liu Tie
Published: December 2021

The authors were not aware of errors that were made during the proofreading phase and would hence wish to make the below-mentioned corrections to this paper [...]
Security Challenges and Air Quality Management in India: Emissions Inventory and Forecasting Estimates
Authors: Khan Haroon ur Rashid , Abbas Shujaat , Anser Muhammad Khalid , Nassani Abdelmohsen A. , Haffar Mohamed , Zaman Khalid
Published: December 2021

The defense and peace literature have focused mainly on the military-growth nexus, with little attention paid to the environmental sustainability agenda, which is impacted by increased global arms transfers. The supply of lead-containing ammunition generates complex gas mixtures (including CO2 emissions) and particulates that harm the healthcare sustainability agenda. Based on the significance of the subject matter, the study uses the Indian economy as a case study, with a significant rate of arms transfers associated with higher carbon emissions. The study analyzed data from more than four decades, from 1975 to 2020. Data on arms imports, military personnel, and military expenditures are used to evaluate the ‘ammunition emissions function’. It corresponds to the three research hypotheses, namely, the ‘emissions-defense burden hypothesis’ (arms transfers increase carbon emissions), the ‘emissions-cleaner hypothesis’ (arms transfers reduce carbon emissions), and the ‘emissions-asymmetric hypothesis’ (positive and negative shocks of arms transfers either support the ‘defense burden hypothesis’ or ‘cleaner hypothesis’). The non-linear autoregressive distributed lag (NARDL) results confirmed the ’emissions-defense burden hypothesis‘ in the long run, as positive and negative shocks from arms imports increase carbon emissions. However, in the short run, positive arms imports increase carbon emissions while negative arms imports decrease carbon emissions. Furthermore, the findings supported the ’emissions-cleaner hypothesis‘ in the relationship between armed forces personnel and carbon emissions. The findings imply that the positive and negative shocks experienced by armed forces personnel reduce carbon emissions in the short and long run. Positive shocks to military spending support the ’emissions-defense burden hypothesis‘ in the short run; however, the results vanished when negative shocks to military spending supported the ’emissions-spillover hypothesis‘ (lowering military spending reduces carbon emissions and increases economic productivity) in the short and long run. The country’s unsustainable economic activities are viewed as a negative factor contributing to long-term carbon emissions increases. The negative shocks of armed forces personnel and positive arms imports would almost certainly have a significant long-term impact on carbon emissions. As a result, the ‘treadmill theory of destruction’ has been confirmed in a country. The study concludes that lead-free ammunition and managing ammunition safety are beneficial to a country’s environmental sustainability agenda.
Blowin’ in the Wind: Wind Dispersal Ability of Phytopathogenic Fusarium in a Wind Tunnel Experiment
Authors: Hoffmann Annika , Funk Roger , Müller Marina E. H.
Published: December 2021

Dispersal processes play an essential role in cereal diseases caused by phytopathogenic Fusarium. However, most empirical studies of Fusarium spore dispersal have focused on vertical transport by rain splash, while wind dispersal has been mostly neglected. Our objective was to determine the ability of Fusarium conidiospores to disperse via wind under controlled conditions in a wind tunnel study. Ten Fusarium species with diverse spore varieties were studied by placing them in the wind stream at wind velocities of 5 and 8 m s−1 and collecting them after 6 m and a period of 1 h using a newly developed air sampling box. Although spore concentrations were high in the releasing Petri Dishes, the tested isolates were recaptured in only 18 of 78 runs. F. equiseti and F. cerealis were the most frequently recovered species. Changing abiotic conditions, wind speed, and spore shapes had no significant effect on Fusarium spore recapture rates. Another experiment showed that conidiospores were rarely released from the grown mycelium. Therefore, the importance of wind alone as a dispersal medium for Fusarium conidiospores may have been overestimated so far. Further studies should investigate the importance of carrier media or mobile linkers combined with the wind dispersal of spores.
Two Extensions of Cover Automata
Authors: Câmpeanu Cezar
Published: December 2021

Deterministic Finite Cover Automata (DFCA) are compact representations of finite languages. Deterministic Finite Automata with “do not care” symbols and Multiple Entry Deterministic Finite Automata are both compact representations of regular languages. This paper studies the benefits of combining these representations to get even more compact representations of finite languages. DFCAs are extended by accepting either “do not care” symbols or considering multiple entry DFCAs. We study for each of the two models the existence of the minimization or simplification algorithms and their computational complexity, the state complexity of these representations compared with other representations of the same language, and the bounds for state complexity in case we perform a representation transformation. Minimization for both models proves to be NP-hard. A method is presented to transform minimization algorithms for deterministic automata into simplification algorithms applicable to these extended models. DFCAs with “do not care” symbols prove to have comparable state complexity as Nondeterministic Finite Cover Automata. Furthermore, for multiple entry DFCAs, we can have a tight estimate of the state complexity of the transformation into equivalent DFCA.
Natural Intervarietal Hybrids of Coffea canephora Have a High Content of Diterpenes
Authors: Francisco Julyene Silva , Dias Rafael Carlos Eloy , Alves Enrique Anastácio , Rocha Rodrigo Barros , Dalazen Janderson Rodrigues , Mori André Luiz Buzzo , Benassi Marta de Toledo
Published: December 2021

The present investigation characterized the diterpene profile of Coffea canephora coffees, which are natural intervarietal hybrids of Conilon and Robusta. The impact of genetic diversity and environment on these compounds was also evaluated. Five genotypes (clones 03, 05, 08, 25, and 66) from six growing sites in the State of Rondônia in the western Amazon (Alto Alegre dos Parecis, São Miguel do Guaporé, Nova Brasilândia do Oeste, Porto Velho, Rolim de Moura, and Alto Paraíso) were analyzed. The contents of kahweol, cafestol, and 16-O-methylcafestol in light-medium roasted coffees were assessed by UPLC. Data were analyzed by ANOVA and Tukey’s test (p ≤ 0.05). The contents of cafestol and 16-O-methylcafestol ranged from 96 to 457 mg 100 g−1 and 75 to 433 mg 100 g−1, respectively. As for kahweol, from absence up to contents of 36.9 mg 100 g−1 was observed. The diterpene profile was dependent on genetics, growing site, and the interaction between these factors. A higher variability was observed for kahweol contents. The natural intervarietal hybrid coffees stood out for their high contents of diterpenes and increased frequency of kahweol presence (77% of the samples).
Developing a Novel Integrated Generalised Data Envelopment Analysis (DEA) to Evaluate Hospitals Providing Stroke Care Services
Authors: Mirmozaffari Mirpouya , Shadkam Elham , Khalili Seyed Mohammad , Yazdani Maziar
Published: December 2021

Stroke is the biggest cause of adult disability and the third biggest cause of death in the US. Stroke is a medical emergency, and the treatment given in the early hours is important in shaping the patient’s long-term recovery and prognosis. Despite the fact that substantial attention has been dedicated to this complex and difficult issue in healthcare, novel strategies such as operation research-based approaches have hardly been used to deal with the difficult challenges associated with stroke. This study proposes a novel approach with data envelopment analysis (DEA) and multi-objective linear programming (MOLP) in hospitals that provide stroke care services to select the most efficient approach, which will be a new experiment in literature perception. DEA and MOLP are widely used for performance evaluation and efficiency measurement. Despite their similarities and common concepts, the two disciplines have evolved separately. The generalised DEA (GDEA) cannot incorporate the preferences of decision-makers (DMs) preferences and historical efficiency data. In contrast, MOLP can incorporate the DM’s preferences into the decision-making process. We transform the GDEA model into MOLP through the max-ordering approach to (i) solve the problem interactively; (ii) use the step method (STEM) and consider DM’s preferences; (iii) eliminate the need for predetermined preference information; and (iv) apply the most preferred solution (MPS) to identify the most efficient approach. A case study of hospitals that provide stroke care services is taken as an example to illustrate the potential application of the proposed approach method.
Metabolic and Transcriptional Changes across Osteogenic Differentiation of Mesenchymal Stromal Cells
Authors: Sigmarsdottir Thora Bjorg , McGarrity Sarah , de Lomana Adrián López García , Kotronoulas Aristotelis , Sigurdsson Snaevar , Yurkovich James T. , Rolfsson Ottar , Sigurjonsson Olafur Eysteinn
Published: December 2021

Mesenchymal stromal cells (MSCs) are multipotent post-natal stem cells with applications in tissue engineering and regenerative medicine. MSCs can differentiate into osteoblasts, chondrocytes, or adipocytes, with functional differences in cells during osteogenesis accompanied by metabolic changes. The temporal dynamics of these metabolic shifts have not yet been fully characterized and are suspected to be important for therapeutic applications such as osteogenesis optimization. Here, our goal was to characterize the metabolic shifts that occur during osteogenesis. We profiled five key extracellular metabolites longitudinally (glucose, lactate, glutamine, glutamate, and ammonia) from MSCs from four donors to classify osteogenic differentiation into three metabolic stages, defined by changes in the uptake and secretion rates of the metabolites in cell culture media. We used a combination of untargeted metabolomic analysis, targeted analysis of 13C-glucose labelled intracellular data, and RNA-sequencing data to reconstruct a gene regulatory network and further characterize cellular metabolism. The metabolic stages identified in this proof-of-concept study provide a framework for more detailed investigations aimed at identifying biomarkers of osteogenic differentiation and small molecule interventions to optimize MSC differentiation for clinical applications.
Merkel Cell Carcinoma: From Pathobiology to Clinical Management
Authors: Farooq Baba Peerzada Umar , Rasool Zubaida , Younas Khan Ishrat , Cockerell Clay J. , Wang Richard , Kassir Martin , Stege Henner , Grabbe Stephan , Goldust Mohamad
Published: December 2021

Merkel cell carcinoma (MCC) is an infrequent, rapidly growing skin neoplasm that carries a greater probability of regional lymph node involvement, and a grim prognosis in advanced cases. While it is seen predominantly in old age in sun-exposed body parts, the prevalence varies among different races and geographical regions. Merkel cell polyomavirus and UV radiation-induced mutations contribute to its etiopathogenesis. The clinical presentation of MCC lacks pathognomonic features and is rarely considered highly at the time of presentation. Histopathological examination frequently reveals hyperchromatic nuclei with high mitotic activity, but immunohistochemistry is required to confirm the diagnosis. Sentinel lymph node biopsy (SLNB) and imaging are advised for effective staging of the disease. Multimodal management including surgery, radiation therapy, and/or immunotherapy are deployed. Traditional cytotoxic chemotherapies may result in an initial response, but do not result in a significant survival benefit. Checkpoint inhibitors have dramatically improved the prognosis of patients with metastatic MCC, and are recommended first-line in advanced cases. There is a need for well-tolerated agents with good safety profiles in patients who have failed immunotherapies.
Exploring the Functionality of Mesh-to-Mesh Value Comparison in Pair-Matching and Its Application to Fragmentary Remains
Authors: McWhirter Zoe , Karell Mara A. , Er Ali , Bozdag Mustafa , Ekizoglu Oguzhan , Kranioti Elena F.
Published: December 2021

Many cases encountered by forensic anthropologists involve commingled remains or isolated elements. Common methods for analysing these contexts are characterised by limitations such as high degrees of subjectivity, high cost of application, or low proven accuracy. This study sought to test mesh-to-mesh value comparison (MCV), a relatively new method for pair-matching skeletal elements, to validate the claims that the technique is unaffected by age, sex and pathology. The sample consisted of 160 three-dimensional clavicle models created from computed tomography (CT) scans of a contemporary Turkish population. Additionally, this research explored the application of MVC to match fragmented elements to their intact counterparts by creating a sample of 480 simulated fragments, consisting of three different types based on the region of the bone they originate from. For comparing whole clavicles, this resulted in a sensitivity value of 87.6% and specificity of 90.9% using ROC analysis comparing clavicles. For the fragment comparisons, each type was compared to the entire clavicles of the opposite side. The results included a range of sensitivity values from 81.3% to 87.6%. Overall results are promising and the MVC technique seems to be a useful technique for matching paired elements that can be accurately applied to a Modern Turkish sample.
Structural Changes and Mechanical Resistance of Claws and Denticles in Coconut Crabs of Different Sizes
Authors: Inoue Tadanobu , Oka Shin-ichiro , Nakazato Koji , Hara Toru
Published: December 2021

The exoskeleton of the pinching side of claws with denticles and of the outer side without them on the coconut crab, Birgus latro, which is a rare organism, were studied using a materials science approach. The mechanical resistance of three claws of different sizes was investigated along the exoskeleton thickness from the outer surface to the inner surface, and the results were compared, including the contribution of the microstructure and chemical compositions. Mechanical properties, hardness (H) and stiffness (Er), were probed through nanoindentation tests. The results showed the H, Er, microstructures, and chemical components of the exocuticle and endocuticle layers were almost the same, in a BW range of 300 g to 1650 g. At the same time, the H and Er near the surface of the denticles of a small coconut crab of 300 g were lower than those of other large coconut crabs. The microstructure of the denticles was clearly different from that of the exocuticle, but the maximum mechanical properties near their surface indicated almost the same values, Hmax = 4 GPa and Er(max) = 70 GPa, regardless of being on the pinching side or the outer side. A denticle can be regarded as a bulge of the cuticle without phosphorus and with high magnesium. The results provided novel information that expanded our knowledge about the claw microstructure of coconut crabs with different body sizes, and may be used in further studies
MS-222 and Propofol Sedation during and after the Simulated Transport of Nile tilapia (Oreochromis niloticus)
Authors: Félix Luís , Correia Rita , Sequeira Rita , Ribeiro Cristiana , Monteiro Sandra , Antunes Luís , Silva José , Venâncio Carlos , Valentim Ana
Published: December 2021

The use of anesthetics has been suggested as a strategy to hamper live fish transport-induced stress. Still, there is insufficient data available on the use of alternative anesthetics to MS-222. This study investigated the use of propofol to mitigate stress in Nile tilapia (Oreochromis niloticus, 143.8 ± 20.9 g and 20.4 ± 0.9 cm) during a 6 h simulated transport. Individuals (n = 7) were divided into three groups: control, 40 mg L−1 MS-222, and 0.8 mg L−1 propofol. A naïve group non-transported was also considered. During the 6 h transport and 24 h after, the response to external stimuli, opercular movements, water quality parameters, behavior, blood hematology and other physiological values, the histopathology of the gills, the quality of the fillet, and oxidative-stress changes in gills, muscle, brain, and liver were evaluated. Propofol increased swimming activity of fish but decreased opercular movements and responses to external stimuli, indicating oscillations of the sedation depth. Water pH and glucose levels increased, while hematocrit (HCT) and lactate decreased in propofol groups at 6 h. At this time-point, MS-222 also induced a decrease in the HCT and lactate levels while increasing cortisol levels. Despite these effects, the stress-related behaviors lessened with anesthetics compared to the control group. After the recovery period, physiological responses normalized in animals from both anesthetic groups, but the control still had high cortisol levels. Overall, propofol is a good alternative for the transportation of this species, showing efficient sedation without compromising health or fillet quality. However, further pharmacodynamics and pharmacokinetics knowledge is required to support its use in aquaculture settings.
DNA Nanodevice-Based Drug Delivery Systems
Authors: Guan Chaoyang , Zhu Xiaoli , Feng Chang
Published: December 2021

DNA, a natural biological material, has become an ideal choice for biomedical applications, mainly owing to its good biocompatibility, ease of synthesis, modifiability, and especially programmability. In recent years, with the deepening of the understanding of the physical and chemical properties of DNA and the continuous advancement of DNA synthesis and modification technology, the biomedical applications based on DNA materials have been upgraded to version 2.0: through elaborate design and fabrication of smart-responsive DNA nanodevices, they can respond to external or internal physical or chemical stimuli so as to smartly perform certain specific functions. For tumor treatment, this advancement provides a new way to solve the problems of precise targeting, controllable release, and controllable elimination of drugs to a certain extent. Here, we review the progress of related fields over the past decade, and provide prospects for possible future development directions.
Investigation of the Effect of Nanocrystalline Calcium Carbonate-Substituted Hydroxyapatite and L-Lysine and L-Arginine Surface Interactions on the Molecular Properties of Dental Biomimetic Composites
Authors: Goloshchapov Dmitry , Kashkarov Vladimir , Nikitkov Kirill , Seredin Pavel
Published: December 2021

Differences in the surface interactions of non-stoichiometric nanocrystalline B-type carbonate-substituted hydroxyapatite (n-cHAp) with the amino acids L-Lysine hydrochloride (L-LysHCl) and L-Arginine hydrochloride (L-ArgHCl) in acidic and alkaline media were determined using structural and spectroscopic analysis methods. The obtained data confirm that hydroxyapatite synthesized using our technique, which was used to develop the n-cHAp/L-LysHCl and n-cHAp/L-ArgHCl composites, is nanocrystalline. Studies of molecular composition of the samples by Fourier transform infrared spectroscopy under the change in the charge state of L-Lysine in environments with different alkalinity are consistent with the results of X-ray diffraction analysis, as evidenced by the redistribution of the modes’ intensities in the spectra that is correlated with the side chains, i.e., amide and carboxyl groups, of the amino acid. During the formation of a biomimetic composite containing L-Lysine hydrochloride and n-cHAp, the interaction occurred through bonding of the L-Lysine side chain and the hydroxyl groups of hydroxyapatite, which created an anionic form of L-Lysine at pH ≤ 5. In contrast, in biocomposites based on L-Arginine and n-cHAp, the interaction only slightly depends on pH value, and it proceeds by molecular orientation mechanisms. The X-ray diffraction and infrared spectroscopy results confirm that changes in the molecular composition of n-cHAp/L-ArgHCl biomimetic composites are caused by the electrostatic interaction between the L-ArgHCl molecule and the carbonate-substituted calcium hydroxyapatite. In this case, the bond formation was detected by Fourier transform infrared (FTIR) spectroscopy; the vibrational modes attributed to the main carbon chain and the guanidine group of L-Arginine are shifted during the interaction. The discovered interaction mechanisms between nanocrystalline carbonate-substituted hydroxyapatite that has physicochemical properties characteristic of the apatite in human dental enamel and specific amino acids are important for selecting the formation conditions of biomimetic composites and their integration with the natural dental tissue.
A Facile and Scalable Hydrogel Patterning Method for Microfluidic 3D Cell Culture and Spheroid-in-Gel Culture Array
Authors: Su Chengxun , Chuah Yon Jin , Ong Hong Boon , Tay Hui Min , Dalan Rinkoo , Hou Han Wei
Published: December 2021

Incorporation of extracellular matrix (ECM) and hydrogel in microfluidic 3D cell culture platforms is important to create a physiological microenvironment for cell morphogenesis and to establish 3D co-culture models by hydrogel compartmentalization. Here, we describe a simple and scalable ECM patterning method for microfluidic cell cultures by achieving hydrogel confinement due to the geometrical expansion of channel heights (stepped height features) and capillary burst valve (CBV) effects. We first demonstrate a sequential “pillar-free” hydrogel patterning to form adjacent hydrogel lanes in enclosed microfluidic devices, which can be further multiplexed with one to two stepped height features. Next, we developed a novel “spheroid-in-gel” culture device that integrates (1) an on-chip hanging drop spheroid culture and (2) a single “press-on” hydrogel confinement step for rapid ECM patterning in an open-channel microarray format. The initial formation of breast cancer (MCF-7) spheroids was achieved by hanging a drop culture on a patterned polydimethylsiloxane (PDMS) substrate. Single spheroids were then directly encapsulated on-chip in individual hydrogel islands at the same positions, thus, eliminating any manual spheroid handling and transferring steps. As a proof-of-concept to perform a spheroid co-culture, endothelial cell layer (HUVEC) was formed surrounding the spheroid-containing ECM region for drug testing studies. Overall, this developed stepped height-based hydrogel patterning method is simple to use in either enclosed microchannels or open surfaces and can be readily adapted for in-gel cultures of larger 3D cellular spheroids or microtissues.
Watershed Brain Regions for Characterizing Brand Equity-Related Mental Processes
Authors: Watanuki Shinya
Published: December 2021

Brand equity is an important intangible for enterprises. As one advantage, products with brand equity can increase revenue, compared with those without such equity. However, unlike tangibles, it is difficult for enterprises to manage brand equity because it exists within consumers’ minds. Although, over the past two decades, numerous consumer neuroscience studies have revealed the brain regions related to brand equity, the identification of unique brain regions related to such equity is still controversial. Therefore, this study identifies the unique brain regions related to brand equity and assesses the mental processes derived from these regions. For this purpose, three analysis methods (i.e., the quantitative meta-analysis, chi-square tests, and machine learning) were conducted. The data were collected in accordance with the general procedures of a qualitative meta-analysis. In total, 65 studies (1412 foci) investigating branded objects with brand equity and unbranded objects without brand equity were examined, whereas the neural systems involved for these two brain regions were contrasted. According to the results, the parahippocampal gyrus and the lingual gyrus were unique brand equity-related brain regions, whereas automatic mental processes based on emotional associative memories derived from these regions were characteristic mental processes that discriminate branded from unbranded objects.
What Do Cognitive Networks Do? Simulations of Spoken Word Recognition Using the Cognitive Network Science Approach
Authors: Vitevitch Michael S. , Mullin Gavin J. D.
Published: December 2021

Cognitive network science is an emerging approach that uses the mathematical tools of network science to map the relationships among representations stored in memory to examine how that structure might influence processing. In the present study, we used computer simulations to compare the ability of a well-known model of spoken word recognition, TRACE, to the ability of a cognitive network model with a spreading activation-like process to account for the findings from several previously published behavioral studies of language processing. In all four simulations, the TRACE model failed to retrieve a sufficient number of words to assess if it could replicate the behavioral findings. The cognitive network model successfully replicated the behavioral findings in Simulations 1 and 2. However, in Simulation 3a, the cognitive network did not replicate the behavioral findings, perhaps because an additional mechanism was not implemented in the model. However, in Simulation 3b, when the decay parameter in spreadr was manipulated to model this mechanism the cognitive network model successfully replicated the behavioral findings. The results suggest that models of cognition need to take into account the multi-scale structure that exists among representations in memory, and how that structure can influence processing.
Deriving Implications for Care Delivery in Parkinson’s Disease by Co-Diagnosing Caregivers as Invisible Patients
Authors: Thieken Franziska , van Munster Marlena
Published: December 2021

For persons with Parkinson’s disease, the loss of autonomy in daily life leads to a high level of dependency on relatives’ support. Such dependency strongly correlates with high levels of perceived stress and psychosocial burden in informal caregivers. Global developments, such as demographic change and the associated thinning infrastructure in rural areas cause a continuously growing need for medical and nursing care. However, this need is not being adequately met. The resulting care gap is being made up by unpaid or underpaid work of informal caregivers. The double burden of care work and gainful employment creates enormous health-related impairments of the informal caregivers, so that they eventually become invisible patients themselves. Expectedly, those invisible patients do not receive the best care, leading to a decrease in quality of life and, in the end, to worse care for PD patients. Suggested solutions to relieve relatives, such as moving the person affected by Parkinson’s to a nursing home, often do not meet the wishes of patients and informal caregivers, nor does it appear as a structural solution in the light of demographic change against an economic background. Rather, it requires the development, implementation and evaluation of new, holistic approaches to care that make invisible patients visible.
Repetitive and Inflexible Active Coping and Addiction-like Neuroplasticity in Stressed Mice of a Helplessness–Resistant Inbred Strain
Authors: Cabib Simona , Campus Paolo , Latagliata Emanuele Claudio , Orsini Cristina , Tarmati Valeria
Published: December 2021

Dysfunctional coping styles are involved in the development, persistence, and relapse of psychiatric diseases. Passive coping with stress challenges (helplessness) is most commonly used in animal models of dysfunctional coping, although active coping strategies are associated with generalized anxiety disorder, social anxiety disorder, panic, and phobias as well as obsessive-compulsive and post-traumatic stress disorder. This paper analyzes the development of dysfunctional active coping strategies of mice of the helplessness–resistant DBA/2J (D2) inbred strain, submitted to temporary reduction in food availability in an uncontrollable and unavoidable condition. The results indicate that food-restricted D2 mice developed a stereotyped form of food anticipatory activity and dysfunctional reactive coping in novel aversive contexts and acquired inflexible and perseverant escape strategies in novel stressful situations. The evaluation of FosB/DeltaFosB immunostaining in different brain areas of food-restricted D2 mice revealed a pattern of expression typically associated with behavioral sensitization to addictive drugs and compulsivity. These results support the conclusion that an active coping style represents an endophenotype of mental disturbances characterized by perseverant and inflexible behavior.
Experimental Study on the Behavior of Steel–Concrete Composite Decks with Different Shear Span-to-Depth Ratios
Authors: Sirimontree Sayan , Thongchom Chanachai , Keawsawasvong Suraparb , Nuaklong Peem , Jongvivatsakul Pitcha , Dokduea Warayut , Bui Linh Van Hong , Farsangi Ehsan Noroozinejad
Published: December 2021

This paper presents the results of an experimental study on the mechanical behaviors of steel‒concrete composite decks with different shear span-to-depth ratios. Herein, four composite decks categorized into two types with shear span-to-depth ratios of 2.5 and 4.6 are designed for an experimental program. The decks then undergo the four-point bending tests until failure to investigate the structural responses, such as the load, displacement, crack mechanism, and failure mode. Conventional section analysis is used to derive the flexural strength of composite decks in comparison with the test results. Additionally, the ductility of the composite decks is assessed based on the displacement indices. The analysis results demonstrate that the stiffness and capacity of the composite deck increase with the decrease in the shear span length. However, the ductility of the composite slabs increases with the shear span length. The flexural strengths predicted by section analysis overestimate the actual test results. The shear span-to-depth ratio affects the crack mechanism of the composite decks.
Influence of Gradation on the Mechanical Properties of Aged Hot-Mix Asphalts
Authors: Chaves-Pabón Saieth Baudilio , Rondón-Quintana Hugo Alexander , Zafra-Mejía Carlos Alfonso
Published: December 2021

When a hot-mix asphalt (HMA) ages, its mechanical properties, resistance, and durability change. Several studies have been conducted throughout the world to evaluate the effects of aging in HMAs. However, few studies have analyzed the influence of gradation. The main objective of this study was to evaluate the influence of gradation on the mechanical properties of aged HMA mixes. For such purposes, three HMA mixes with different gradations were manufactured (named HMA-10, HMA-19, and HMA-25), which were conditioned in STOA (short-term oven aging) and LTOA (long-term oven aging) by following the guidelines established by the AASHTO R30 specification. Marshall, Indirect Tensile Strength (ITS), resilient modulus, permanent deformation, fatigue (under controlled-stress condition), and Cantabro tests were performed. These tests were carried out to evaluate resistance under monotonic and cyclic load as well as the resistance to moisture damage and abrasion. The best performing mix in the test was HMA-19. There is no clear trend about the influence of gradation over the susceptibility of mixes for aging. This susceptibility changes depending on the test performed and the property measured. Furthermore, in resilient modulus tests, it is observed that the influence of gradation on aging susceptibility changes depending on the test temperature.
An Exhaustive Search Energy Optimization Method for Residential Building Envelope in Different Climatic Zones of Kazakhstan
Authors: Kaderzhanov Mirzhan , Memon Shazim Ali , Saurbayeva Assemgul , Kim Jong R.
Published: December 2021

Nowadays, the residential sector of Kazakhstan accounts for about 30% of the total energy consumption. Therefore, it is essential to analyze the energy estimation model for residential buildings in Kazakhstan so as to reduce energy consumption. This research is aimed to develop the Overall Thermal Transfer Value (OTTV) based Building Energy Simulation Model (BESM) for the reduction of energy consumption through the envelope of residential buildings in seven cities of Kazakhstan. A brute force optimization method was adopted to obtain the optimal envelope configuration varying window-to-wall ratio (WWR), the angle of a pitched roof, the depth of the overhang shading system, the thermal conductivity, and the thicknesses of wall composition materials. In addition, orientation-related analyses of the optimized cases were conducted. Finally, the economic evaluation of the base and optimized cases were presented. The results showed that an average energy reduction for heating was 6156.8 kWh, while for cooling it was almost 1912.17 kWh. The heating and cooling energy savings were 16.59% and 16.69%, respectively. The frontage of the building model directed towards the south in the cold season and north in the hot season demonstrated around 21% and 32% of energy reduction, respectively. The energy cost savings varied between 9657 to 119,221 ₸ for heating, 9622 to 36,088 ₸ for cooling.
Planting Season Impacts Sugarcane Stem Development, Secondary Metabolite Levels, and Natural Antisense Transcription
Authors: Wijma Maryke , Lembke Carolina Gimiliani , Diniz Augusto Lima , Santini Luciane , Zambotti-Villela Leonardo , Colepicolo Pio , Carneiro Monalisa Sampaio , Souza Glaucia Mendes
Published: December 2021

To reduce the potentially irreversible environmental impacts caused by fossil fuels, the use of renewable energy sources must be increased on a global scale. One promising source of biomass and bioenergy is sugarcane. The study of this crop’s development in different planting seasons can aid in successfully cultivating it in global climate change scenarios. The sugarcane variety SP80-3280 was field grown under two planting seasons with different climatic conditions. A systems biology approach was taken to study the changes on physiological, morphological, agrotechnological, transcriptomics, and metabolomics levels in the leaf +1, and immature, intermediate and mature internodes. Most of the variation found within the transcriptomics and metabolomics profiles is attributed to the differences among the distinct tissues. However, the integration of both transcriptomics and metabolomics data highlighted three main metabolic categories as the principal sources of variation across tissues: amino acid metabolism, biosynthesis of secondary metabolites, and xenobiotics biodegradation and metabolism. Differences in ripening and metabolite levels mainly in leaves and mature internodes may reflect the impact of contrasting environmental conditions on sugarcane development. In general, the same metabolites are found in mature internodes from both “one-year” and “one-and-a-half-year sugarcane”, however, some metabolites (i.e., phenylpropanoids with economic value) and natural antisense transcript expression are only detected in the leaves of “one-year” sugarcane.
Porous Functional Graded Bioceramics with Integrated Interface Textures
Authors: Biggemann Jonas , Köllner David , Simon Swantje , Heik Paula , Hoffmann Patrizia , Fey Tobias
Published: December 2021

Porous functional graded ceramics (porous FGCs) offer immense potential to overcome the low mechanical strengths of homogeneously porous bioceramics used as bone grafts. The tailored manipulation of the graded pore structure including the interfaces in these materials is of particular interest to locally control the microstructural and mechanical properties, as well as the biological response of the potential implant. In this work, porous FGCs with integrated interface textures were fabricated by a novel two-step transfer micro-molding technique using alumina and hydroxyapatite feedstocks with varied amounts of spherical pore formers (0–40 Vol%) to generate well-defined porosities. Defect-free interfaces could be realized for various porosity pairings, leading to porous FGCs with continuous and discontinuous transition of porosity. The microstructure of three different periodic interface patterns (planar, 2D-linear waves and 3D-Gaussian hills) was investigated by SEM and µCT and showed a shape accurate replication of the CAD-designed model in the ceramic sample. The Young’s modulus and flexural strength of bi-layered bending bars with 0 and 30 Vol% of pore formers were determined and compared to homogeneous porous alumina and hydroxyapaite containing 0–40 Vol% of pore formers. A significant reduction of the Young’s modulus was observed for the porous FGCs, attributed to damping effects at the interface. Flexural 4-point-testing revealed that the failure did not occur at the interface, but rather in the porous 30 Vol% layer, proving that the interface does not represent a source of weakness in the microstructure.
Association of Infant Feeding Practices with Iron Status and Hematologic Parameters in 6-Month-Old Infants
Authors: Ruangkit Chayatat , Prachakittikul Nawapat , Hemprachitchai Nutthida , Dumrongwongsiri Oraporn , Soonsawad Sasivimon
Published: December 2021

Background: Infants’ feeding practices in the first 6 months of life and their association with iron status and hematologic parameters has not been well studied. We aim to evaluate this association. Methods: In a retrospective chart review, we identified 403 infants who received laboratory screening for anemia at 6-month visits. Infants were categorized into four groups according to feeding practices. Hematologic parameters and incidence of anemia, iron deficiency (ID), and iron deficiency anemia (IDA) were compared. Results: In total, 105 infants were breastfed (BF), 78 were breastfed with iron supplementation starting at 4 months (BI), 109 were mixed-fed (breast milk and formula) with or without iron supplementation (MF), and 111 were formula-fed (FF). The BF group had the highest incidence of anemia (38.1%), ID (28.6%), and IDA (17.1%) when compared with the other groups (p < 0.001). In multivariate logistic regression, BI, MF, and FF infants had 90.4%, 97.5%, and 96.9% decreased risk of IDA, respectively, with BF infants as a reference group. Conclusion: The incidence of anemia, ID, and IDA at age 6 months was higher in BF than FF or MF infants. However, iron supplements in BF infants starting at 4 months significantly reduced their ID and IDA incidence.
Patterns of Father Involvement and Child Development among Families with Low Income
Authors: Yoon Susan , Kim Minjung , Yang Junyeong , Lee Joyce Y. , Latelle Anika , Wang Jingyi , Zhang Yiran , Schoppe-Sullivan Sarah
Published: December 2021

This study examined patterns of father involvement and their relations with social, behavioral, and cognitive development among low-income children < 5 years. Latent class analysis on data from 2650 fathers (Mage = 29.35 years) in the Supporting Healthy Marriages program revealed four father involvement patterns: (1) High positive involvement (48%); (2) engaged but harsh discipline (42%); (3) low cognitive stimulation (8%); and (4) lower involvement (2%). The low cognitive stimulation pattern was associated with greater father- and mother-reported child behavior problems and lower child socioemotional and cognitive functioning. The engaged but harsh discipline pattern was associated with more father-reported child behavior problems. These findings highlight the need for active engagement of fathers in parenting interventions to promote child development.