bluuebunny/crossref_metadata_2025 huggingface dataset:
The Impact of Land Transfers on the Adoption of New Varieties: Evidence from Micro-Survey Data in Shaanxi Province, China
Authors: Chen Yi , Wang Zhengbing
Published: March 2023

Land transfers play a vital role in determining the level of farm machinery service and variety selection by scattered land reduction and contiguous land enhancement, which are also conducive to poverty alleviation and welfare utilization. Based on the micro-survey data of 898 kiwifruit growers in Shaanxi Province, this paper analyzed the effect of land transfers on the adoption of new varieties by propensity score matching (PSM) technology. A stepwise regression method was used to test the mediating role of land scale and the moderating role of unmanned aerial vehicles (UAVs). The results suggest the following: (1) Farmers with land transfers had a significant positive effect on the adoption of new varieties, with a 12.5% increase in the likelihood of new variety adoption. (2) The impact of land transfers on the adoption of new varieties was partially mediated through land scale. (3) The positive moderating role of UAV services was empirically emphasized. With the goal of promoting the average income and boosting farmland productivity, the improvement of the land property rights trading market, joint efforts from government and rural cooperatives, and professional and regular training are proposed to optimize land-use structures and reduce machinery service costs, thereby encouraging farmers to adopt new, improved varieties.
Quantitative Evaluation of Runoff Simulation and Its Driving Forces Based on Hydrological Model and Multisource Precipitation Fusion
Authors: Ma Zice , Yao Rui , Sun Peng , Zhuang Zhen , Ge Chenhao , Zou Yifan , Lv Yinfeng
Published: March 2023

The hydrological cycle across the source regions of the Yellow River (SRYR) affects water supply for 324 million people across the Yellow River basin (YRB), and the scarcity of meteorological stations leads to great challenges for the estimation of hydrologic and energy balance. Therefore, our work employs multisource precipitation products across the YRB to develop a new integrated precipitation product with the optimized Bayesian mean algorithm (OBMA). It investigates the performance and hydrological utility of the optimal Bayesian integrated precipitation product (OBIPP). This study found that the OBIPP improved by 14.08% in overall performance relative to the optimal precipitation product across the SRYR, respectively. Meanwhile, the variable infiltration capacity (VIC) model, driven by daily OBIPP, can drastically improve the accuracy of runoff simulation compared with other precipitation products across the SRYR. According to the VIC model driven by daily OBIPP, the average precipitation and runoff depth across the SRYR were approximately 621 mm and 64 mm from 2001 to 2019, respectively, showing a spatial trend increasing from northwest to southeast. Overall, OBIPP is characterized by smaller uncertainty of simulation and higher simulation performance across the SRYR, which should provide a scientific basis for accurate prediction and assessment of water resources in areas where meteorological data are scarce.
Effects of Main Land-Use Types on Plant and Microbial Diversity and Ecosystem Multifunctionality in Degraded Alpine Grasslands
Authors: Su Hongye , Ma Li , Chang Tao , Qin Ruimin , Zhang Zhonghua , She Yandi , Wei Jingjing , Zhou Chenyu , Hu Xue , Shi Zhengchen , Adi Haze , Li Honglin , Zhou Huakun
Published: March 2023

Grassland resources occupy an important place in the national economy. However, grasslands in alpine regions of China are severely degraded, and the effects of land-use types on species composition, soil nutrients, and ecosystem multifunctionality of degraded alpine grasslands are less certain. To ascertain the effects of main land-use types (no-tillage reseeding and fertilization) on species diversity and ecosystem multifunctionality in alpine grasslands, we investigated the changes in these factors by subjecting specified areas. Using a standardized field survey, we measured the cover, richness, and evenness of plants. At each site, we measured microbial diversity and twelve soil variables critical for maintaining ecosystem multifunctionality in alpine grasslands. The results showed that: (1) the Margalef, Shannon–Wiener, and Simpson indices of plant community, and fungal diversity indices increased significantly in no-tillage reseeding and fertilization; (2) at the phyla level, the relative abundances of Basidiomycota, Olpidiomycota, and Proteobacteria increased significantly in no-tillage reseeding and fertilization, as well as, at the genus level, those of Coniochaeta, Solirubrobacter, Pseudonocardia, and Microvirga; (3) the soil physicochemical properties (except the C:N of soil) increased significantly in no-tillage reseeding and fertilization; (4) correlation analysis showed that species diversity was mainly correlated with soil nutrients in control check, while it was mainly correlated with soil physical properties in no-tillage reseeding and fertilization; (5) linear regression analysis showed significant positive relationships between Margalef, Shannon–Wiener, and Simpson indices of plant community and ecosystem multifunctionality. In addition, ecosystem multifunctionality was positively related to Pielou, Shannon–Wiener, and Simpson indices of the fungal community and it was positively related to Pielou and Shannon–Wiener indices of bacterial community. These observations indicated that no-tillage reseeding and fertilization of degraded alpine grasslands had the potential to improve ecosystem functions in many ways.
The Eye as a Diagnostic Tool for Alzheimer’s Disease
Authors: Hussain Ahsan , Sheikh Zahra , Subramanian Manju
Published: March 2023

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder impacting cognition, function, and behavior in the elderly population. While there are currently no disease-modifying agents capable of curing AD, early diagnosis and management in the preclinical stage can significantly improve patient morbidity and life expectancy. Currently, the diagnosis of Alzheimer’s disease is a clinical one, often supplemented by invasive and expensive biomarker testing. Over the last decade, significant advancements have been made in our understanding of AD and the role of ocular tissue as a potential biomarker. Ocular biomarkers hold the potential to provide noninvasive and easily accessible diagnostic and monitoring capabilities. This review summarizes current research for detecting biomarkers of Alzheimer’s disease in ocular tissue.
Assessment of Dynamic Surface Leaching of Monolithic Polymer Mortars Comprised of Wastes
Authors: Maherzi Walid , Ennahal Ilyas , Bouaich Fatima Zahra , Benzerzour Mahfoud , Rais Zakia , Mamindy-Pajany Yannick , Abriak Nor-Edine
Published: March 2023

Today, the reuse of waste in building materials occupies an important place in the approach to the circularity of materials. National and European environmental regulations require ensuring the environmental safety of material-incorporating waste. For this, there are specific tests to verify that there is no health risk when using these materials. Concretely, to check the environmental acceptability of construction materials, including wastes, the release of hazardous substances into water must be assessed. In this research, we performed a diffusion test with the sequential renewal of water during a 64-day period according to the NF EN 15863 specifications on polymer mortar monoliths, common construction products used in floor-covering applications and incorporating sediments. Polymer mortars were prepared at a laboratory scale by incorporating 30 or 50% of polluted sediment for various polymer concentrations (12, 14, 16, 18, 20 and 25%). It was shown that the release of inorganic substances is limited in these hydrodynamic conditions. Among trace elements, As, Cd, Cr, Ni, Pb and Zn are lower than quantification limits in most leachates, whereas Ba, Co, Cu and V are systematically quantified at low concentration levels. This is particularly true for samples displaying the highest polymer concentration (25%) and the lowest sediment incorporation rate (30%). This is because of the low water absorption level and low porosity of polymer mortar matrices. No adverse effect is to be expected for environmental health from the leachates of these construction materials, including waterways sediments, because all the measured parameters were below the Soil Quality Decree limits applied in the Netherlands for environmental assessment of construction products.
Influence of Fumed Nanosilica on Ballistic Performance of UHPCs
Authors: Markusík David , Bocian Luboš , Novotný Radoslav , Palovčík Jakub , Hrbáčová Markéta
Published: March 2023

This research delves into the potential use of fumed nanosilica in ultra-high performance concrete for ballistic protection. First, the mechanical properties, slump flow, and specific gravity of UHPC with different contents of Aerosil 200 were determined. Then, calorimetric studies were conducted on these cement composites. Lastly, the differential efficiency factor and spalling area of UHPC with fumed nanosilica were determined. It was found out that the slump flow, the mechanical properties, and differential efficiency factor are slightly decreased by the addition of fumed nanosilica. However, the addition of the fumed nanosilica is beneficial in terms of the spalling area decrease and it is highly reactive during the induction period. Some of the results are supported by BSEM imaging.
Study on the Effect of Temperature on the Crystal Transformation of Microporous Calcium Silicate Synthesized of Extraction Silicon Solution from Fly Ash
Authors: Kang Dong , Yang Zhijie , Zhang De , Jiao Yang , Fang Chenyang , Wang Kaiyue
Published: March 2023

In this study, microporous calcium silicate was synthesized from a silicon solution of fly ash extracted by soaking in strong alkali as a silicon source. By means of XRD, TEM, FTIR, and thermodynamic calculations, the crystal evolution and growth process of microporous calcium silicate were studied under the synthesis temperature of 295~365 K. The results show that calcium silicate is a single-chain structure of the Si–O tetrahedron: Q1 type Si–O tetrahedron is located at both ends of the chain, and the middle is the [SiO44−] tetrahedron connected by [O2−] coplanar, and Ca2+ is embedded in the interlayer structure of calcium silicate. The formation rate and crystallization degree of calcium silicate hydrate were positively correlated with temperature. When the synthesis temperature was 295 K, its particle size was about 8 μm, and when the synthesis temperature was 330 K, a large number of amorphous microporous calcium silicate with a particle size of about 14 μm will be generated. When the temperature was above 350 K, the average particle size was about 17 μm. The microporous calcium silicate showed obvious crystalline characteristics, which indicate that the crystallization degree and particle size of microporous calcium silicate could be controlled by a reasonable synthesis temperature adjustment.
Clinically Relevant Properties of 3D Printable Materials for Intraoral Use in Orthodontics: A Critical Review of the Literature
Authors: Goracci Cecilia , Juloski Jovana , D’Amico Claudio , Balestra Dario , Volpe Alessandra , Juloski Jelena , Vichi Alessandro
Published: March 2023

The review aimed at analyzing the evidence available on 3D printable materials and techniques used for the fabrication of orthodontic appliances, focusing on materials properties that are clinically relevant. MEDLINE/PubMed, Scopus, and Cochrane Library databases were searched. Starting from an initial retrieval of 669 citations, 47 articles were finally included in the qualitative review. Several articles presented proof-of-concept clinical cases describing the digital workflow to manufacture a variety of appliances. Clinical studies other than these case reports are not available. The fabrication of aligners is the most investigated application of 3D printing in orthodontics, and, among materials, Dental LT Clear Resin (Formlabs) has been tested in several studies, although Tera Harz TC-85 (Graphy) is currently the only material specifically marketed for direct printing of aligners. Tests of the mechanical properties of aligners materials lacked homogeneity in the protocols, while biocompatibility tests failed to assess the influence of intraoral conditions on eluents release. The aesthetic properties of 3D-printed appliances are largely unexplored. The evidence on 3D-printed metallic appliances is also limited. The scientific evidence on 3D printable orthodontic materials and techniques should be strengthened by defining international standards for laboratory testing and by starting the necessary clinical trials.
Ageing Evaluation of Foamed Polymer Modified Bitumen with Bio-Flux Additive
Authors: Radziszewski Piotr , Liphardt Adam , Sarnowski Michał , Kowalski Karol J. , Pokorski Piotr , Konieczna Katarzyna , Król Jan B. , Iwański Marek , Chomicz-Kowalska Anna , Maciejewski Krzysztof , Iwański Mateusz M. , Michalec Maciej
Published: March 2023

This paper presents the results of an analysis of the changes in the stiffness of asphalt binders modified with a bio-flux additive and subjected to the processes of foaming and short-term ageing. The purpose of the analyses was to determine changes in the rheological properties of asphalt binder as a result of technological processes characteristic of hot and warm mix asphalt technology. Three asphalt binders with similar penetration but varying degrees of polymer modification were tested: 50/70, 45/80–55 polymer-modified bitumen, and 45/80–80 highly modified bitumen. Tests were carried out on four groups of binders: original binders, foamed binders after 14 days of storage, non-foamed binders after Rolling Thin Film Oven Test (RTFOT) ageing, and foamed binders after 14 days of storage subjected to RTFOT ageing. The master curves of the complex shear modulus G* were analysed, and three indexes of binder stiffening were determined, characterising the investigated effects. The tests showed that some of the stiffening indices significantly depended on the degree of polymer modification and the content of the bio-flux additive. Moreover, it was found that the foaming process in the case of paving-grade bitumen and polymer-modified bitumen did not contribute to the additional stiffening of the binders.
Homogeneity and Thermal Stability of Sputtered Al0.7Sc0.3N Thin Films
Authors: Carmona-Cejas José Manuel , Mirea Teona , Nieto Jesús , Olivares Jimena , Felmetsger Valery , Clement Marta
Published: March 2023

This work presents a study on the homogeneity and thermal stability of Al0.7Sc0.3N films sputtered from Al-Sc segmented targets. The films are sputtered on Si substrates to assess their structural properties and on SiO2/Mo-based stacked acoustic mirrors to derive their piezoelectric activity from the frequency response of acoustic resonators. Post-deposition annealing at temperatures up to 700 °C in a vacuum are carried out to test the stability of the Al0.7Sc0.3N films and their suitability to operate at high temperatures. Despite the relatively constant radial composition of the films revealed from RBS measurements, a severe inhomogeneity in the piezoelectric activity is observed across the wafer, with significantly poorer activity in the central zone. RBS combined with NRA analysis shows that the zones of lower piezoelectric activity are likely to show higher surface oxygen adsorption, which is attributed to higher ion bombardment during the deposition process, leading to films with poorer crystalline structures. AFM analysis reveals that the worsening of the material properties in the central area is also accompanied by an increased roughness. XRD analysis also supports this hypothesis, even suggesting the possibility of a ScN non-piezoelectric phase coexisting with the AlScN piezoelectric phase. Thermal treatments do not result in great improvements in terms of piezoelectric activity and crystalline structure.
Painting the Black Box White: Experimental Findings from Applying XAI to an ECG Reading Setting
Authors: Cabitza Federico , Campagner Andrea , Natali Chiara , Parimbelli Enea , Ronzio Luca , Cameli Matteo
Published: March 2023

The emergence of black-box, subsymbolic, and statistical AI systems has motivated a rapid increase in the interest regarding explainable AI (XAI), which encompasses both inherently explainable techniques, as well as approaches to make black-box AI systems explainable to human decision makers. Rather than always making black boxes transparent, these approaches are at risk of painting the black boxes white, thus failing to provide a level of transparency that would increase the system’s usability and comprehensibility, or even at risk of generating new errors (i.e., white-box paradox). To address these usability-related issues, in this work we focus on the cognitive dimension of users’ perception of explanations and XAI systems. We investigated these perceptions in light of their relationship with users’ characteristics (e.g., expertise) through a questionnaire-based user study involved 44 cardiology residents and specialists in an AI-supported ECG reading task. Our results point to the relevance and correlation of the dimensions of trust, perceived quality of explanations, and tendency to defer the decision process to automation (i.e., technology dominance). This contribution calls for the evaluation of AI-based support systems from a human–AI interaction-oriented perspective, laying the ground for further investigation of XAI and its effects on decision making and user experience.
A Robust Fractional-Order Control Scheme for PV-Penetrated Grid-Connected Microgrid
Authors: Pachauri Nikhil , Thangavel Vigneysh , Suresh Velamuri , Kantipudi Mvv Prasad , Kotb Hossam , Tripathi Ravi Nath , Bajaj Mohit
Published: March 2023

This article presents a new cascaded control strategy to control the power flow in a renewable-energy-based microgrid operating in grid-connected mode. The microgrid model is composed of an AC utility grid interfaced with a multi-functional grid interactive converter (MF-GIC) acting as a grid-forming converter, a photovoltaic (PV) power-generation system acting as grid-feeding distributed generation unit, and various sensitive/non-sensitive customer loads. The proposed control strategy consists of a fractional order PI (FO-PI) controller to smoothly regulate the power flow between the utility grid, distributed generation unit, and the customers. The proposed controller exploits the advantages of FO (Fractional Order) calculus in improving the steady-state and dynamic performance of the renewable-energy-based microgrid under various operating conditions and during system uncertainties. To tune the control parameters of the proposed controller, a recently developed evaporation-rate-based water-cycle algorithm (ERWCA) is utilized. The performance of the proposed control strategy is tested under various operating conditions to show its efficacy over the conventional controller. The result shows that the proposed controller is effective and robust in maintaining all the system parameters within limits under all operating conditions, including system uncertainties.
On the Borderline of Fields and Hyperfields
Authors: Massouros Christos G. , Massouros Gerasimos G.
Published: March 2023

The hyperfield came into being due to a mathematical necessity that appeared during the study of the valuation theory of the fields by M. Krasner, who also defined the hyperring, which is related to the hyperfield in the same way as the ring is related to the field. The fields and the hyperfields, as well as the rings and the hyperrings, border on each other, and it is natural that problems and open questions arise in their boundary areas. This paper presents such occasions, and more specifically, it introduces a new class of non-finite hyperfields and hyperrings that is not isomorphic to the existing ones; it also classifies finite hyperfields as quotient hyperfields or non-quotient hyperfields, and it gives answers to the question that was raised from the isomorphic problems of the hyperfields: when can the subtraction of a field F’s multiplicative subgroup G from itself generate F? Furthermore, it presents a construction of a new class of hyperfields, and with regard to the problem of the isomorphism of its members to the quotient hyperfields, it raises a new question in field theory: when can the subtraction of a field F’s multiplicative subgroup G from itself give all the elements of the field F, except the ones of its multiplicative subgroup G?
A Soft Robot Driven by a Spring-Rolling Dielectric Elastomer Actuator with Two Bristles
Authors: Du Yangyang , Wu Xiaojun , Xue Jiasheng , Chen Xingyu , Cao Chongjing , Gao Xing
Published: March 2023

Confined space searches such as pipeline inspections are widely demanded in various scenarios, where lightweight soft robots with inherent compliance to adapt to unstructured environments exhibit good potential. We proposed a tubular soft robot with a simple structure of a spring-rolled dielectric elastomer (SRDE) and compliant passive bristles. Due to the compliance of the bristles, the proposed robots can work in pipelines with inner diameters both larger and smaller than the one of the bristles. Firstly, the nonlinear dynamic behaviors of the SRDE were investigated experimentally. Then, we fabricated the proposed robot with a bristle diameter of 19 mm and then studied its performance in pipelines on the ground with inner diameters of 18 mm and 20 mm. When the pipeline’s inner diameter was less than the outer diameter of the bristles, the bristles remained in the state of bending and the robot locomotion is mainly due to anisotropic friction (1.88 and 0.88 body lengths per second horizontally and vertically, respectively, in inner diameter of 18 mm and 0.06 body length per second in that of 16 mm). In the case of the pipeline with the larger inner diameter, the bristles were not fully constrained, and a small bending moment applied on the lower bristle legs contributed to the robot’s locomotion, leading to a high velocity (2.78 body lengths per second in 20 mm diameter acrylic pipe). In addition, the robot can work in varying geometries, such as curving pipes (curve radius ranges from 0.11 m to 0.31 m) at around two body lengths per second horizontally and on the ground at 3.52 body lengths per second, showing promise for pipeline or narrow space inspections.
Antiviral Potential of Selected N-Methyl-N-phenyl Dithiocarbamate Complexes against Human Immunodeficiency Virus (HIV)
Authors: Mufhandu Hazel T. , Obisesan Oluwafemi S. , Ajiboye Timothy O. , Mhlanga Sabelo D. , Onwudiwe Damian C.
Published: March 2023

Despite the use of highly active antiretroviral therapy approved by the United States Food and Drug Administration (FDA) for the treatment of human immunodeficiency virus (HIV) infection, HIV remains a public health concern due to the inability of the treatment to eradicate the virus. In this study, N-methyl-N-phenyl dithiocarbamate complexes of indium(III), bismuth(III), antimony(III), silver(I), and copper(II) were synthesized. The complexes were characterized by thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), and Fourier transform infrared spectroscopy (FTIR). The N-methyl-N-phenyl dithiocarbamate complexes were then evaluated for their antiviral effects against HIV-1 subtypes A (Q168), B (QHO.168), and C (CAP210 and ZM53). The results showed that the copper(II)-bis (N-methyl-N-phenyl dithiocarbamate) complex had a neutralization efficiency of 94% for CAP210, 54% for ZM53, 45% for Q168, and 63% for QHO.168. The silver(I)-bis (N-methyl-N-phenyl dithiocarbamate) complex showed minimal neutralization efficiency against HIV, while indium(III) and antimony(III) N-methyl-N-phenyl dithiocarbamate complexes had no antiviral activity against HIV-1. The findings revealed that copper(II)-bis (N-methyl-N-phenyl dithiocarbamate), with further improvement, could be explored as an alternative entry inhibitor for HIV.
LncRNA 8244-ssc-miR-320-CCR7 Regulates IFN-β during SVA Infecting PK-15 Cells
Authors: Tang Xiaoyu , Zhang Ruiyu , Gao Long , Lv Xiaocheng , Sun Yuan , Ma Jingyun
Published: March 2023

Seneca Valley virus (SVV), a member of the Picornaviridae family, is an oncolytic RNA virus that can cause idiopathic vesicular disease and increase mortality in newborn piglets. Although research on the pathogenic characteristics, epidemiology, pathogenic mechanism, and clinical diagnosis of SVA has increased due to its emergence and prevalence, the interaction between SVA and its host lncRNA has not been fully studied. This study used qualcomm sequencing to analyze differentially expressed lncRNAs and found that during SVA infection, lncRNA 8244 was significantly down-regulated in both PK-15 cells and piglets. Further analysis through quantitative real-time PCR and dual luciferase experiments demonstrated that lncRNA8244 could compete with ssc-miR-320 to regulate the expression of CCR7. The lncRNA824-ssc-miR-320-CCR7 axis activated the TLR-mediated signaling pathway, which recognized viral molecules and induced the expression of IFN-β. These findings provide new insight into the interaction between lncRNA and SVA infection, which could lead to a better understanding of SVA pathogenesis and contribute to the prevention and control of SVA disease.
Substantiation of New Reagent Compositions for the Effective Extraction of Rhenium in the Processing of Complex Molybdenum Ores
Authors: Chanturiya Valentine A. , Matveeva Tamara N. , Getman Viktoriya V. , Karkeshkina Anna Yu. , Gromova Nadezhda K.
Published: March 2023

Modern trends in technological improvement for producing high-quality rhenium-containing molybdenum concentrates are aimed at developing environmentally friendly solutions com-pared to traditional ones, involving a reduction in consumption or complete replacement of toxic substances and the use of plant reagents and microorganisms and make it possible to eliminate energy-intensive processes of steaming and roasting. It is known that up to 25%–30% of Mo, Cu and Re goes to rough flotation tailings and is lost in wastes. Those losses are a consequence of the ore composition variability, chalcopyrite–molybdenite ratio, an increase in the proportion between primary copper sulfide and finely disseminated molybdenite and the recovery of floating pyrite. High rates of valuable metal recovery into bulk concentrate can be achieved by using new compositions of flotation reagents that selectively change the hydrophobic properties of the target minerals. The application of new reagent compositions, including novel chemicals—dithiopyrylmethane (DTM), composite reagent (CR) and conventional butyl xanthate (ButX)—was theoretically and experimentally substantiated for the effective rhenium extraction in the processing of hard-to-beneficiate complex molybdenum ores. It is identified that DTM forms a complex DTM–Re compound and chemically adsorbed on rhenium-containing molybdenite providing an increase in Re recovery into the bulk Cu–Mo–Re concentrate by 17%, reducing by two times the loss of rhenium with flotation tailings, and the subsequent 97.6% Re extraction of the molybdenum concentrate by autoclave leaching.
The Investigation of the Chemical Composition and Applicability of Gold Nanoparticles Synthesized with Amygdalus communis (Almond) Leaf Aqueous Extract as Antimicrobial and Anticancer Agents
Authors: Baran Mehmet Fırat , Keskin Cumali , Baran Ayşe , Eftekhari Aziz , Omarova Sabina , Khalilov Rovshan , Adican Mehmet Tevfik , Rosić Gvozden , Selakovic Dragica , Yıldıztekin Mahmut , Kurt Kadri , Aytuğ Ava Canan , Atalar Mehmet Nuri
Published: March 2023

The current work’s main objective was to determine the chemical composition of Amygdalus communis (AC) leaf extract and examine the antibacterial and cytotoxic properties of biosynthesized gold nanoparticles (AuNPs). The chemical composition of AC leaf extract was determined using LC-ESI/MS/MS to detect compounds that may be responsible for the reducing, stabilizing, and capping steps in the synthesis of nanoparticles and their biological activities. The AC-AuNPs were spherical, with a particle size lower than 100 nm and a face-centered cubic structure. The EDX spectrum confirmed the formation of AuNPs and a negative zeta potential value (−27.7 mV) suggested their physicochemical stability. The in vitro cytotoxic efficacy of the AC-AuNPs against colorectal adenocarcinoma (Caco-2), glioma (U118), and ovarian (Skov-3) cancer cell lines and human dermal fibroblasts (HDFs) was evaluated by MTT assay. CaCo-2 cell proliferation was effectively inhibited by the AC-AuNPs at concentrations between 25 and 100 g mL−1. The AC-AuNPs exerted preeminent antimicrobial activity against Bacillus subtilis with an MIC of 0.02 μg/mL, whilst good activity was shown against Staphylococcus aureus bacteria and Candida albicans yeast with an MIC of 0.12 μg/mL. Ultimately, the results support the high antibacterial and anticancer potential of biosynthesized AuNPs from AC leaf extract.
Photoactivatable BODIPYs for Live-Cell PALM
Authors: Zhang Yang , Zheng Yeting , Tomassini Andrea , Singh Ambarish Kumar , Raymo Françisco M.
Published: March 2023

Photoactivated localization microscopy (PALM) relies on fluorescence photoactivation and single-molecule localization to overcome optical diffraction and reconstruct images of biological samples with spatial resolution at the nanoscale. The implementation of this subdiffraction imaging method, however, requires fluorescent probes with photochemical and photophysical properties specifically engineered to enable the localization of single photoactivated molecules with nanometer precision. The synthetic versatility and outstanding photophysical properties of the borondipyrromethene (BODIPY) chromophore are ideally suited to satisfy these stringent requirements. Specifically, synthetic manipulations of the BODIPY scaffold can be invoked to install photolabile functional groups and photoactivate fluorescence under photochemical control. Additionally, targeting ligands can be incorporated in the resulting photoactivatable fluorophores (PAFs) to label selected subcellular components in live cells. Indeed, photoactivatable BODIPYs have already allowed the sub-diffraction imaging of diverse cellular substructures in live cells using PALM and can evolve into invaluable analytical probes for bioimaging applications.
Diphenyl Diselenide-Assisted Radical Addition Reaction of Diphenyl Disulfide to Unsaturated Bonds upon Photoirradiation
Authors: Yamamoto Yuki , Chen Qiqi , Ogawa Akiya
Published: March 2023

The addition reaction of interelement compounds with heteroatom–heteroatom single bonds to unsaturated bonds under photoirradiation is an important method for the efficient and atom-economical construction of carbon–heteroatom bonds. However, in practice, the desired addition reaction is sometimes unable to proceed as expected due to the low efficiency of the desired addition reactions or the preferential polymerization of unsaturated compounds. In this study, by combining an interelement compound with homologous heteroatom compounds as a catalyst, we succeeded in suppressing the polymerization of the unsaturated compounds and in attaining a highly selective carbon–heteroatom bond formation through the desired addition reaction. In this paper, we have examined in detail whether such a “catalytic radical reaction” proceeds for unsaturated compounds and found that the dithiolation of some unsaturated compounds (i.e., vinylic ethers, styrenes, and isocyanides) could proceed with the assistance of (PhSe)2 under light. The developed methods in this study are expected to have strong implications in the fields of radical chemistry, heteroatom chemistry, synthetic organic chemistry, and catalyst chemistry as atom-economical methods for carbon–heteroatom bond formation.
Doxorubicin- and Trastuzumab-Modified Gold Nanoparticles as Potential Multimodal Agents for Targeted Therapy of HER2+ Cancers
Authors: Żelechowska-Matysiak Kinga , Wawrowicz Kamil , Wierzbicki Mateusz , Budlewski Tadeusz , Bilewicz Aleksander , Majkowska-Pilip Agnieszka
Published: March 2023

Recently, targeted nanoparticles (NPs) have attracted much attention in cancer treatment due to their high potential as carriers for drug delivery. In this article, we present a novel bioconjugate (DOX–AuNPs–Tmab) consisting of gold nanoparticles (AuNPs, 30 nm) attached to chemotherapeutic agent doxorubicin (DOX) and a monoclonal antibody, trastuzumab (Tmab), which exhibited specific binding to HER2 receptors. The size and shape of synthesized AuNPs, as well as their surface modification, were analyzed by the TEM (transmission electron microscopy) and DLS (dynamic light scattering) methods. Biological studies were performed on the SKOV-3 cell line (HER2+) and showed high specificity of binding to the receptors and internalization capabilities, whereas MDA-MB-231 cells (HER2−) did not. Cytotoxicity experiments revealed a decrease in the metabolic activity of cancer cells and surface area reduction of spheroids treated with DOX–AuNPs–Tmab. The bioconjugate induced mainly cell cycle G2/M-phase arrest and late apoptosis. Our results suggest that DOX–AuNPs–Tmab has great potential for targeted therapy of HER2-positive tumors.
The Potent G-Quadruplex-Binding Compound QN-302 Downregulates S100P Gene Expression in Cells and in an In Vivo Model of Pancreatic Cancer
Authors: Ahmed Ahmed A. , Greenhalf William , Palmer Daniel H. , Williams Nicole , Worthington Jenny , Arshad Tariq , Haider Shozeb , Alexandrou Effrosyni , Guneri Dilek , Waller Zoe A. E. , Neidle Stephen
Published: March 2023

The naphthalene diimide compound QN-302, designed to bind to G-quadruplex DNA sequences within the promoter regions of cancer-related genes, has high anti-proliferative activity in pancreatic cancer cell lines and anti-tumor activity in several experimental models for the disease. We show here that QN-302 also causes downregulation of the expression of the S100P gene and the S100P protein in cells and in vivo. This protein is well established as being involved in key proliferation and motility pathways in several human cancers and has been identified as a potential biomarker in pancreatic cancer. The S100P gene contains 60 putative quadruplex-forming sequences, one of which is in the promoter region, 48 nucleotides upstream from the transcription start site. We report biophysical and molecular modeling studies showing that this sequence forms a highly stable G-quadruplex in vitro, which is further stabilized by QN-302. We also report transcriptome analyses showing that S100P expression is highly upregulated in tissues from human pancreatic cancer tumors, compared to normal pancreas material. The extent of upregulation is dependent on the degree of differentiation of tumor cells, with the most poorly differentiated, from more advanced disease, having the highest level of S100P expression. The experimental drug QN-302 is currently in pre-IND development (as of Q1 2023), and its ability to downregulate S100P protein expression supports a role for this protein as a marker of therapeutic response in pancreatic cancer. These results are also consistent with the hypothesis that the S100P promoter G-quadruplex is a potential therapeutic target in pancreatic cancer at the transcriptional level for QN-302.
Terpenic Constituents of Essential Oils with Larvicidal Activity against Aedes Aegypti: A QSAR and Docking Molecular Study
Authors: Cruz-Castillo Adrián Ulises , Rodríguez-Valdez Luz María , Correa-Basurto José , Nogueda-Torres Benjamín , Andrade-Ochoa Sergio , Nevárez-Moorillón Guadalupe Virginia
Published: March 2023

Aedes aegypti is a vector for the arbovirus responsible for yellow fever, Zika and Chikungunya virus. Essential oils and their constituents are known for their larvicidal properties and are strong candidates for mosquito control. This work aimed to develop a quantitative structure–activity study and molecular screening for the search and design of new larvicidal agents. Twenty-five monoterpenes with previously evaluated larvicidal activity were built and optimized using computational tools. QSAR models were constructed through genetic algorithms from the larvicidal activity and the calculation of theoretical descriptors for each molecule. Docking studies on acetylcholinesterase (AChE) and sterol carrier protein (SCP-2) were also carried out. Results demonstrate that the epoxide groups in the structure of terpenes hinder larvicidal activity, while lipophilicity plays an important role in enhancing biological activity. Larvicidal activity correlates with the interaction of the sterol-carrier protein. Of the 25 compounds evaluated, carvacrol showed the highest larvicidal activity with an LC50 of 8.8 µg/mL. The information included in this work contributes to describing the molecular, topological, and quantum mechanical properties related to the larvicidal activity of monoterpenes and their derivatives.
Bioassay-Guided Alkaloids Isolation from Camellia sinensis and Colchicum luteum: In Silico and In Vitro Evaluations for Protease Inhibition
Authors: Aatif Mohammad , Raza Muhammad Asam , El Oirdi Mohamed , Farhan Mohd , Mumtaz Muhammad Waseem , Hamayun Muhammad , Ashraf Adnan , Muteeb Ghazala
Published: March 2023

Bioassay-guided isolation from Camellia sinensis (Theaceae) and Colchicum luteum (Liliaceae) utilizing an in vitro model of protease assay revealed colchicine (1) and caffeine (2) from chloroform fractions, respectively. Their structures were validated using spectral techniques. The purified compounds were further optimized with Gaussian software utilizing the B3LYP functional and 6-31G(d,p) basis set. The result files were utilized to determine several global reactivity characteristics to explain the diverse behavior of the compounds. Colchicine (1) showed a higher inhibition of protease activity (63.7 ± 0.5 %age with IC50 = 0.83 ± 0.07 mM), compared with caffeine (2) (39.2 ± 1.3 %age). In order to determine the type of inhibition, compound 1 was further studied, and, based on Lineweaver–Burk/Dixon plots and their secondary replots, it was depicted that compound 1 was a non-competitive inhibitor of this enzyme, with a Ki value of 0.690 ± 0.09 mM. To elucidate the theoretical features of protease inhibition, molecular docking studies were performed against serine protease (PDB #1S0Q), which demonstrated that compound 1 had a strong interaction with the different amino acid residues located on the active site of this understudied enzyme, with a high docking score of 16.2 kcal/mol.
Optimization, Probiotic Characteristics, and Rheological Properties of Exopolysaccharides from Lactiplantibacillus plantarum MC5
Authors: Zhao Xuefang , Liang Qi
Published: March 2023

This study optimized the exopolysaccharides (EPS) production for Lactiplantibacillus plantarum MC5 (Lp. plantarum MC5) and evaluated the resistance to human simulated digestive juices, antioxidant activity in vitro, and rheological properties of EPS-MC5. The results showed that maximum EPS production of 345.98 mg/L (about 1.5-old greater than the initial production) was obtained at optimal conditions of inoculum size (4.0%), incubation time (30 h), incubation temperature (34.0 °C), and initial pH value (6.40). Furthermore, the resisting-digestion capacity of EPS-MC5 after 180 min in α-amylase, simulated gastric juice (pH 2.0, 3.0, 4.0), and simulated intestinal juice (pH 6.8) was 98.59%, 98.62%, 98.78%, 98.86%, and 98.74%, respectively. In addition, the radical scavenging rates of DPPH•, ABTS•, •OH, and ferric-iron reducing power (OD700) of EPS-MC5 were 73.33%, 87.74%, 46.07%, and 1.20, respectively. Furthermore, rheological results showed that the EPS-MC5 had a higher apparent viscosity (3.01 Pa) and shear stress (41.78 Pa), and the viscoelastic modulus (84.02 and 161.02 Pa at the shear frequency of 100 Hz). These results provide a new insight into the application of EPS in human health and functional foods, which could also improve theoretical guidance for the industrial application of EPS.
Enhanced Tensile Properties, Biostability, and Biocompatibility of Siloxane–Cross-Linked Polyurethane Containing Ordered Hard Segments for Durable Implant Application
Authors: Wu Xiaofei , Jia Hanxiao , Fu Wenshuo , Li Meng , Pan Yitong
Published: March 2023

This work developed a series of siloxane-modified polyurethane (PU–Si) containing ordered hard segments by a facile method. The polyaddition between poly(ε-caprolactone) and excess diurethane diisocyanate was carried out to synthesize a polyurethane prepolymer with terminal isocyanate groups, which was then end-capped by 3-aminopropyl triethoxysilane to produce alkoxysilane-terminated polyurethane; the target products of PU–Si were obtained with hydrolysis and the condensation of alkoxysilane groups. The chemical structures were confirmed by FT-IR and XPS, and the effect of the siloxane content or cross-linked degree on the physicochemical properties of the PU–Si films was investigated in detail. The formation of the network structure linked by Si–O–Si bonds and interchain denser hydrogen bonds endowed PU–Si films with fine phase compatibility, low crystallinity, high thermal stability, and excellent tensile properties. Due to the high cross-linked degree and low interfacial energy, the films displayed a high surface water contact angle and low equilibrium water absorption, which resulted in slow hydrolytic degradation rates. Furthermore, the evaluation of protein adsorption and platelet adhesion on the PU–Si film surface presented high resistance to biofouling, indicating superior surface biocompatibility. Consequently, the siloxane–cross-linked polyurethane, which possessed excellent tensile properties, high biostability, and superior biocompatibility, showed great potential to be explored as biomaterials for durable implants.
Molecular Beam Epitaxy Growth of Quantum Wires and Quantum Dots
Authors: Li Xiaohui , Xu Qian , Zhang Ziyang
Published: March 2023

Molecular beam epitaxy technology has a significant advantage in semiconductor technology due to its strong controllability, especially for the preparation of materials such as quantum wires and quantum dots [...]
Nanobionics: A Sustainable Agricultural Approach towards Understanding Plant Response to Heavy Metals, Drought, and Salt Stress
Authors: Faizan Mohammad , Karabulut Fadime , Alam Pravej , Yusuf Mohammad , Tonny Sadia Haque , Adil Muhammad Faheem , Sehar Shafaque , Ahmed S. Maqbool , Hayat Shamsul
Published: March 2023

In the current scenario, the rising concentration of heavy metals (HMs) due to anthropogenic activities is a severe problem. Plants are very much affected by HM pollution as well as other abiotic stress such as salinity and drought. It is very important to fulfil the nutritional demands of an ever-growing population in these adverse environmental conditions and/or stresses. Remediation of HM in contaminated soil is executed through physical and chemical processes which are costly, time-consuming, and non-sustainable. The application of nanobionics in crop resilience with enhanced stress tolerance may be the safe and sustainable strategy to increase crop yield. Thus, this review emphasizes the impact of nanobionics on the physiological traits and growth indices of plants. Major concerns and stress tolerance associated with the use of nanobionics are also deliberated concisely. The nanobionic approach to plant physiological traits and stress tolerance would lead to an epoch of plant research at the frontier of nanotechnology and plant biology.
Nutritional Assessment of Plant-Based Meat Alternatives: A Comparison of Nutritional Information of Plant-Based Meat Alternatives in Spanish Supermarkets
Authors: Rizzolo-Brime Lucía , Orta-Ramirez Alicia , Puyol Martin Yael , Jakszyn Paula
Published: March 2023

Since the classification of processed meat as carcinogenic by the International Agency for Research on Cancer (IARC) in 2015, an increase in consumption of plant-based meat alternatives (PBMAs) has been observed worldwide. This occurs in a context characterized by concern for health, animal welfare, and sustainability; however, evidence of their nutritional quality is still limited. Therefore, our objective was to evaluate the nutritional profile and processing degree of PBMAs available in Spain. In 2020, products from seven Spanish supermarkets were analyzed for their nutritional content and ingredients. Of the 148 products, the majority were low in sugars but moderate in carbohydrates, total and saturated fat, and high in salt. The main vegetable protein sources were soy (91/148) and wheat gluten (42/148). Comparatively, 43/148 contained animal protein, the most common being egg. Overall, PBMAs had a long list of ingredients and additives, and they were classified as ultra-processed foods (UPFs) according to the NOVA system. This study shows that the PBMAs available in Spanish supermarkets have a variable nutritional composition within and between categories. Further research is needed to determine if replacing meat with these UPFs could be a good alternative towards healthier and more sustainable dietary patterns.
The Role of Functionalization and Size of Gold Nanoparticles in the Response of MCF-7 Breast Cancer Cells to Ionizing Radiation Comparing 2D and 3D In Vitro Models
Authors: Musielak Marika , Boś-Liedke Agnieszka , Piwocka Oliwia , Kowalska Katarzyna , Markiewicz Roksana , Szymkowiak Barbara , Bakun Paweł , Suchorska Wiktoria M.
Published: March 2023

Gold nanoparticles (AuNPs), as an agent enhancing radiosensitivity, play a key role in the potential treatment of breast cancer (BC). Assessing and understanding the kinetics of modern drug delivery systems is a crucial element that allows the implementation of AuNPs in clinical treatment. The main objective of the study was to assess the role of the properties of gold nanoparticles in the response of BC cells to ionizing radiation by comparing 2D and 3D models. In this research, four kinds of AuNPs, different in size and PEG length, were used to sensitize cells to ionizing radiation. The in vitro viability, uptake, and reactive oxygen species generation in cells were investigated in a time- and concentration-dependent manner using 2D and 3D models. Next, after the previous incubation with AuNPs, cells were irradiated with 2 Gy. The assessment of the radiation effect in combination with AuNPs was analyzed using the clonogenic assay and γH2AX level. The study highlights the role of the PEG chain in the efficiency of AuNPs in the process of sensitizing cells to ionizing radiation. The results obtained imply that AuNPs are a promising solution for combined treatment with radiotherapy.