bluuebunny/crossref_metadata_2025 huggingface dataset:
Active Laser-Camera Scanning for High-Precision Fruit Localization in Robotic Harvesting: System Design and Calibration
Authors: Zhang Kaixiang , Chu Pengyu , Lammers Kyle , Li Zhaojian , Lu Renfu
Published: December 2023

Robust and effective fruit detection and localization is essential for robotic harvesting systems. While extensive research efforts have been devoted to improving fruit detection, less emphasis has been placed on the fruit localization aspect, which is a crucial yet challenging task due to limited depth accuracy from existing sensor measurements in the natural orchard environment with variable lighting conditions and foliage/branch occlusions. In this paper, we present the system design and calibration of an Active LAser-Camera Scanner (ALACS), a novel perception module for robust and high-precision fruit localization. The hardware of the ALACS mainly consists of a red line laser, an RGB camera, and a linear motion slide, which are seamlessly integrated into an active scanning scheme where a dynamic-targeting laser-triangulation principle is employed. A high-fidelity extrinsic model is developed to pair the laser illumination and the RGB camera, enabling precise depth computation when the target is captured by both sensors. A random sample consensus-based robust calibration scheme is then designed to calibrate the model parameters based on collected data. Comprehensive evaluations are conducted to validate the system model and calibration scheme. The results show that the proposed calibration method can detect and remove data outliers to achieve robust parameter computation, and the calibrated ALACS system is able to achieve high-precision localization with the maximum depth measurement error being less than 4 mm at distance ranging from 0.6 to 1.2 m.
Effect of Biofertilizers on Broccoli Yield and Soil Quality Indicators
Authors: Ollio Irene , Santás-Miguel Vanesa , Gómez Diego Soto , Lloret Eva , Sánchez-Navarro Virginia , Martínez-Martínez Silvia , Egea-Gilabert Catalina , Fernández Juan A. , Calviño David Fernández , Zornoza Raúl
Published: December 2023

High rates of fertilizer applications potentially have significant environmental consequences, such as soil and water contamination and biodiversity loss. This study aimed to compare the use of biofertilizers and inorganic fertilizers in a broccoli crop to determine their impact on soil microorganism abundance, microbial community structure, functional gene diversity, yield, and greenhouse gas emissions. Four different fertilization treatments were designed: (i) inorganic fertilizers applied at a rate to cover the nutritional demands of the crop (F100); (ii) 50% of the rate of inorganic fertilizers added in F100 (F50); (iii) F50 + the application of a formulation of various bacteria (BA); and (iv) F50 + the application of a formulation of bacteria and non-mycorrhizal fungi (BA + FU). The results showed that reduced fertilization and the addition of both biofertilizer products had no significant effect on soil nutrients, microbial population, microbial activity, or yield when compared to conventional inorganic fertilization. Thus, microbial inoculants were ineffective in enhancing soil microbial abundance and activity, and there were no changes in GHG emissions or crop yields. Nonetheless, crop yield was positively related to total soil N, microbial activity, and CO2 emissions, confirming the positive effect of soil biodiversity on production. The application of biofertilizers can help reduce mineral fertilization in a broccoli crop with no negative effect on yield.
Agricultural Water Footprints and Productivity in the Colorado River Basin
Authors: Frisvold George B. , Duval Dari
Published: December 2023

The Colorado River provides water to 40 million people in the U.S. Southwest, with river basin spanning 250,000 square miles (647,497 km2). Quantitative water rights assigned to U.S. states, Mexico, and tribes in the Colorado Basin exceed annual streamflows. Climate change is expected to limit streamflows further. To balance water demands with supplies, unprecedented water-use cutbacks have been proposed, primarily for agriculture, which consumes more than 60% of the Basin’s water. This study develops county-level, Basin-wide measures of agricultural economic water productivity, water footprints, and irrigation cash rent premiums, to inform conservation programs and compensation schemes. These measures identify areas where conservation costs in terms of foregone crop production or farm income are high or low. Crop sales averaged USD 814 per acre foot (AF) (USD 0.66/m3) of water consumed in the Lower Basin and 131 USD/AF (USD 0.11/m3) in the Upper Basin. Crop sales minus crop-specific input costs averaged 485 USD/AF (USD 0.39/m3) in the Lower Basin and 93 USD/AF (USD 0.08 per m3) in the Upper Basin. The blue water footprint (BWF) was 1.2 AF/USD 1K (1480 m3/USD1K) of water per thousand dollars of crop sales in the Lower Basin and 7.6 AF/USD 1K (9374 m3/USD1K) in the Upper Basin. Counties with higher water consumption per acre have a lower BWF.
Pilot Testing Two Versions of a Social Network Intervention to Increase HIV Testing and Case-finding among Men in South Africa’s Generalized HIV Epidemic
Authors: Williams Leslie D. , van Heerden Alastair , Ntinga Xolani , Nikolopoulos Georgios K. , Paraskevis Dimitrios , Friedman Samuel R.
Published: December 2023

Locating undiagnosed HIV infections is important for limiting transmission. However, there is limited evidence about how best to do so. In South Africa, men have been particularly challenging to reach for HIV testing due, in part, to stigma. We pilot-tested two versions of a network-based case-finding and care-linkage intervention. The first, TRIP, asked “seeds” (original participants) to recruit their sexual and/or injection partners. The second, TRIPLE, aimed to circumvent some stigma-related issues by asking seeds to recruit anyone they know who might be at risk of being HIV-positive-unaware. We recruited 11 (18% male) newly diagnosed HIV-positive (NDP) seeds from two clinics in KwaZulu-Natal, South Africa and randomly assigned them to either TRIP or TRIPLE. Network members were recruited two steps from each seed. The TRIP arm recruited 12 network members; the TRIPLE arm recruited 62. Both arms recruited NDPs at higher rates than local clinic testing, with TRIP (50.0%) outperforming (p = 0.012) TRIPLE (14.5%). However, TRIPLE (53.2%) was far superior to clinics (27.8%) and to TRIP (25.0%) at recruiting men. Given challenges around testing and treating men for HIV in this context, these findings suggest that the TRIPLE expanded network-tracing approach should be tested formally among larger samples in multiple settings.
Effect of Training for an Athletic Challenge on Illness Cognition in Individuals with Chronic Disability: A Prospective Cohort Study
Authors: DeShazo Joy M. , Kouwijzer Ingrid , de Groot Sonja , Post Marcel W. M. , Valent Linda J. M. , van Leeuwen Christel M. C. , Wen Huacong , Cowan Rachel E. ,   on behalf of the HandbikeBattle Group
Published: December 2023

Illness cognitions (IC) influence how a patient adapts to a chronic disease. The aim was (1) to determine if training for a handcycling mountain time trial (HandbikeBattle) improves IC and (2) to identify factors associated with IC change scores. Persons with a chronic disability (N = 220; including N = 151 with spinal cord disorder) trained 5 months and participated in the time trial. The IC Questionnaire measured helplessness, acceptance, perceived benefits and was assessed before training (T1), after training (T2), and four months after the event (T3). Age, sex, body mass index (BMI), time since injury (TSI), disability characteristics, self-efficacy, mental health (MH) and musculoskeletal pain were obtained at T1. Multilevel regression analyses showed that helplessness decreased (from 11.96 to 11.28, p < 0.01) and perceived benefits increased (from 16.91 to 17.58, p < 0.01) from T1 to T2. For helplessness this decrease persisted during follow-up (11.16 at T3). Changes in helplessness were associated with self-efficacy (p = 0.02), MH (p = 0.02) and lesion completeness (p = 0.02), and were independent of disability type (p = 0.66), lesion level (p = 0.30) and demographics such as sex (p = 0.29) and age (p = 0.67). Training with peers may improve helplessness and perceived benefits in individuals with a chronic disability. Especially individuals with MH problems might benefit from training for an athletic challenge with peers to improve illness cognitions, and ultimately, quality of life.
Cardiovascular Responses to Eccentric Cycling Based on Perceived Exertion Compared to Concentric Cycling, Effect of Pedaling Rate, and Sex
Authors: Faivre-Rampant Victorien , Rakobowchuk Mark , Tordi Nicolas , Mourot Laurent
Published: December 2023

Interest in eccentric exercises has increased over the last decades due to its efficiency in achieving moderate–high intensity muscular work with reduced metabolic demands. However, individualizing eccentric exercises in rehabilitation contexts remains challenging, as concentric exercises mainly rely on cardiovascular parameters. To overcome this, perceived exertion could serve as an individualization tool, but the knowledge about cardiovascular responses to eccentric cycling based on perceived exertion are still scarce. For this purpose, the cardiorespiratory parameters of 26 participants were assessed during two 5 min bouts of concentric cycling at 30 and 60 rpm and two bouts of eccentric cycling at 15 and 30 rpm matched for rating of perceived exertion. With this method, we hypothesized higher exercise efficiency during eccentric cycling for a same perceived exertion. The results revealed significantly elevated heart rate and cardiac index at higher pedalling rates during concentric (p < 0.001), but not during eccentric cycling (p ≈ 1). Exercise efficiency was higher during concentric cycling (64%), decreasing with pedalling rate, while eccentric cycling exhibited increased work rates (82%), and increased by over 100% with higher pedalling rate. Hence, eccentric cycling, with lower cardiorespiratory work for the same perceived exertion, facilitates higher work rates in deconditioned populations. However, further studies are needed for effective individualization.
Developing a Base Domain Ontology from Geoscience Report Collection to Aid in Information Retrieval towards Spatiotemporal and Topic Association
Authors: Tao Liufeng , Ma Kai , Tian Miao , Hui Zhenyang , Zheng Shuai , Liu Junjie , Xie Zhong , Qiu Qinjun
Published: December 2023

The efficient and precise retrieval of desired information from extensive geological databases is a prominent and pivotal focus within the realm of geological information services. Conventional information retrieval methods primarily rely on keyword matching approaches, which often overlook the contextual and semantic aspects of the keywords, consequently impeding the retrieval system’s ability to accurately comprehend user query requirements. To tackle this challenge, this study proposes an ontology-driven information-retrieval framework for geological data that integrates spatiotemporal and topic associations. The framework encompasses the development of a geological domain ontology, extraction of key information, establishment of a multi-feature association and retrieval framework, and validation through a comprehensive case study. By employing the proposed framework, users are empowered to actively and automatically retrieve pertinent information, simplifying the information access process, mitigating the burden of comprehending information organization and software application models, and ultimately enhancing retrieval efficiency.
Towards Topological Geospatial Conflation: An Optimized Node-Arc Conflation Model for Road Networks
Authors: Lei Zhen , Lei Ting L.
Published: December 2023

Geospatial data conflation is the process of identifying and merging the corresponding features in two datasets that represent the same objects in reality. Conflation is needed in a wide range of geospatial analyses, yet it is a difficult task, often considered too unreliable and costly due to various discrepancies between GIS data sources. This study addresses the reliability issue of computerized conflation by developing stronger optimization-based conflation models for matching two network datasets with minimum discrepancy. Conventional models match roads on a feature-by-feature basis. By comparison, we propose a new node-arc conflation model that simultaneously matches road-center lines and junctions in a topologically consistent manner. Enforcing this topological consistency increases the reliability of conflation and reduces false matches. Similar to the well-known rubber-sheeting method, our model allows for the use of network junctions as “control” points for matching network edges. Unlike rubber sheeting, the new model is automatic and matches all junctions (and edges) in one pass. To the best of our knowledge, this is the first optimized conflation model that can match nodes and edges in one model. Computational experiments using six road networks in Santa Barbara, CA, showed that the new model is selective and reduces false matches more than existing optimized conflation models. On average, it achieves a precision of 94.7% with over 81% recall and achieves a 99.4% precision when enhanced with string distances.
Regulation of Satellite Cells Functions during Skeletal Muscle Regeneration: A Critical Step in Physiological and Pathological Conditions
Authors: Careccia Giorgia , Mangiavini Laura , Cirillo Federica
Published: December 2023

Skeletal muscle regeneration is a complex process involving the generation of new myofibers after trauma, competitive physical activity, or disease. In this context, adult skeletal muscle stem cells, also known as satellite cells (SCs), play a crucial role in regulating muscle tissue homeostasis and activating regeneration. Alterations in their number or function have been associated with various pathological conditions. The main factors involved in the dysregulation of SCs’ activity are inflammation, oxidative stress, and fibrosis. This review critically summarizes the current knowledge on the role of SCs in skeletal muscle regeneration. It examines the changes in the activity of SCs in three of the most common and severe muscle disorders: sarcopenia, muscular dystrophy, and cancer cachexia. Understanding the molecular mechanisms involved in their dysregulations is essential for improving current treatments, such as exercise, and developing personalized approaches to reactivate SCs.
Comprehensive Analysis of MICALL2 Reveals Its Potential Roles in EGFR Stabilization and Ovarian Cancer Cell Invasion
Authors: Xia Tianxiang , Ye Fengwen , Zhao Weizhen , Min Pengxiang , Qi Chenxiang , Wang Qianwen , Zhao Mingyu , Zhang Yujie , Du Jun
Published: December 2023

Molecules interacting with CasL (MICALs) are critical mediators of cell motility that act by cytoskeleton rearrangement. However, the molecular mechanisms underlying the regulation of cancer cell invasion remain elusive. The aim of this study was to investigate the potential role of one member of MICALs, i.e., MICALL2, in the invasion and function of ovarian cancer cells. We showed by bioinformatics analysis that MICALL2 expression was significantly higher in tissues of advanced-stage ovarian cancer and associated with poor overall survival of patients. MICALL2 was strongly correlated with the infiltration of multiple types of immune cells and T-cell exhaustion markers. Moreover, enrichment analyses showed that MICALL2 was involved in the tumor-related matrix degradation pathway. Mechanistically, MMP9 was identified as the target gene of MICALL2 for the regulation of invadopodium formation and SKOV3, HO-8910PM cell invasion. In addition, EGFR–AKT–mTOR signaling was identified as the downstream pathway of MICALL2 in the regulation of MMP9 expression. Furthermore, MICALL2 silencing promoted EGFR degradation; however, this effect was abrogated by treatment with the autophagy inhibitors acadesine and chloroquine diphosphate. Silencing of MICALL2 resulted in a suppressive activity of Rac1 while suppressing Rac1 activation attenuated the pro-EGFR, pro-MMP9, and proinvasive effects induced by the overexpression of MICALL2. Collectively, our results indicated that MICALL2 participated in the process of immune infiltration and invasion by ovarian cancer cells. Moreover, MICALL2 prevented EGFR degradation in a Rac1-dependent manner, consequently leading to EGFR–AKT–mTOR–MMP9 signaling activation and invadopodia-mediated matrix degradation.
Serotonergic Modulation of the Excitation/Inhibition Balance in the Visual Cortex
Authors: Carlos-Lima Estevão , Higa Guilherme Shigueto Vilar , Viana Felipe José Costa , Tamais Alicia Moraes , Cruvinel Emily , Borges Fernando da Silva , Francis-Oliveira José , Ulrich Henning , De Pasquale Roberto
Published: December 2023

Serotonergic neurons constitute one of the main systems of neuromodulators, whose diffuse projections regulate the functions of the cerebral cortex. Serotonin (5-HT) is known to play a crucial role in the differential modulation of cortical activity related to behavioral contexts. Some features of the 5-HT signaling organization suggest its possible participation as a modulator of activity-dependent synaptic changes during the critical period of the primary visual cortex (V1). Cells of the serotonergic system are among the first neurons to differentiate and operate. During postnatal development, ramifications from raphe nuclei become massively distributed in the visual cortical area, remarkably increasing the availability of 5-HT for the regulation of excitatory and inhibitory synaptic activity. A substantial amount of evidence has demonstrated that synaptic plasticity at pyramidal neurons of the superficial layers of V1 critically depends on a fine regulation of the balance between excitation and inhibition (E/I). 5-HT could therefore play an important role in controlling this balance, providing the appropriate excitability conditions that favor synaptic modifications. In order to explore this possibility, the present work used in vitro intracellular electrophysiological recording techniques to study the effects of 5-HT on the E/I balance of V1 layer 2/3 neurons, during the critical period. Serotonergic action on the E/I balance has been analyzed on spontaneous activity, evoked synaptic responses, and long-term depression (LTD). Our results pointed out that the predominant action of 5-HT implies a reduction in the E/I balance. 5-HT promoted LTD at excitatory synapses while blocking it at inhibitory synaptic sites, thus shifting the Hebbian alterations of synaptic strength towards lower levels of E/I balance.
Toxicity of Metal Oxide Nanoparticles: Looking through the Lens of Toxicogenomics
Authors: Boyadzhiev Andrey , Wu Dongmei , Avramescu Mary-Luyza , Williams Andrew , Rasmussen Pat , Halappanavar Sabina
Published: December 2023

The impact of solubility on the toxicity of metal oxide nanoparticles (MONPs) requires further exploration to ascertain the impact of the dissolved and particulate species on response. In this study, FE1 mouse lung epithelial cells were exposed for 2–48 h to 4 MONPs of varying solubility: zinc oxide, nickel oxide, aluminum oxide, and titanium dioxide, in addition to microparticle analogues and metal chloride equivalents. Previously published data from FE1 cells exposed for 2–48 h to copper oxide and copper chloride were examined in the context of exposures in the present study. Viability was assessed using Trypan Blue staining and transcriptomic responses via microarray analysis. Results indicate material solubility is not the sole property governing MONP toxicity. Transcriptional signaling through the ‘HIF-1α Signaling’ pathway describes the response to hypoxia, which also includes genes associated with processes such as oxidative stress and unfolded protein responses and represents a conserved response across all MONPs tested. The number of differentially expressed genes (DEGs) in this pathway correlated with apical toxicity, and a panel of the top ten ranked DEGs was constructed (Hmox1, Hspa1a, Hspa1b, Mmp10, Adm, Serpine1, Slc2a1, Egln1, Rasd1, Hk2), highlighting mechanistic differences among tested MONPs. The HIF-1α pathway is proposed as a biomarker of MONP exposure and toxicity that can help prioritize MONPs for further evaluation and guide specific testing strategies.
Developing Pharmacological Therapies for Atrial Fibrillation Targeting Mitochondrial Dysfunction and Oxidative Stress: A Scoping Review
Authors: Menezes Júnior Antônio da Silva , França-e-Silva Ana Luísa Guedes de , Oliveira Joyce Monteiro de , Silva Daniela Melo da
Published: December 2023

Atrial fibrillation (AF) is a cardiac arrhythmia caused by electrophysiological anomalies in the atrial tissue, tissue degradation, structural abnormalities, and comorbidities. A direct relationship exists between AF and altered mitochondrial activity resulting from membrane potential loss, contractile dysfunction, or decreased ATP levels. This review aimed to elucidate the role of mitochondrial oxidative mechanisms in AF pathophysiology, the impact of mitochondrial oxidative stress on AF initiation and perpetuation, and current therapies. This review followed the Preferred Reporting Items for Systematic Reviews and the Meta-Analysis Extension for Scoping Reviews. PubMed, Excerpta Medica Database, and Scopus were explored until June 2023 using “MESH terms”. Bibliographic references to relevant papers were also included. Oxidative stress is an imbalance that causes cellular damage from excessive oxidation, resulting in conditions such as AF. An imbalance in reactive oxygen species production and elimination can cause mitochondrial damage, cellular apoptosis, and cardiovascular diseases. Oxidative stress and inflammation are intrinsically linked, and inflammatory pathways are highly correlated with the occurrence of AF. AF is an intricate cardiac condition that requires innovative therapeutic approaches. The involvement of mitochondrial oxidative stress in the pathophysiology of AF introduces novel strategies for clinical treatment.
The Plastic Interplay between Lung Regeneration Phenomena and Fibrotic Evolution: Current Challenges and Novel Therapeutic Perspectives
Authors: Lettieri Sara , Bertuccio Francesco R. , del Frate Lucia , Perrotta Fabio , Corsico Angelo G. , Stella Giulia M.
Published: December 2023

Interstitial lung diseases (ILDs) are a heterogeneous group of pulmonary disorders characterized by variable degrees of inflammation, interstitial thickening, and fibrosis leading to distortion of the pulmonary architecture and gas exchange impairment. Among them, idiopathic pulmonary fibrosis (IPF) displays the worst prognosis. The only therapeutic options consist of the two antifibrotic drugs, pirfenidone and nintedanib, which limit fibrosis progression but do not reverse the lung damage. The shift of the pathogenetic paradigm from inflammatory disease to epithelium-derived disease has definitively established the primary role of type II alveolar cells, which lose their epithelial phenotype and acquire a mesenchymal phenotype with production of collagen and extracellular matrix (EMC) deposition. Some predisposing environmental and genetic factors (e.g., smoke, pollution, gastroesophageal reflux, variants of telomere and surfactant genes) leading to accelerated senescence set a pro-fibrogentic microenvironment and contribute to the loss of regenerative properties of type II epithelial cells in response to pathogenic noxae. This review provides a complete overview of the different pathogenetic mechanisms leading to the development of IPF. Then, we summarize the currently approved therapies and the main clinical trials ongoing. Finally, we explore the potentialities offered by agents not only interfering with the processes of fibrosis but also restoring the physiological properties of alveolar regeneration, with a particular focus on potentialities and concerns about cell therapies based on mesenchymal stem cells (MSCs), whose anti-inflammatory and immunomodulant properties have been exploited in other fibrotic diseases, such as graft versus host disease (GVHD) and COVID-19-related ARDS.
Long-Term Exposure to Supraphysiological Levels of Testosterone Impacts Rat Submandibular Gland Proteome
Authors: Valente-Santos João , Vitorino Rui , Sousa-Mendes Cláudia , Oliveira Paula , Colaço Bruno , Faustino-Rocha Ana I. , Neuparth Maria João , Leite-Moreira Adelino , Duarte José Alberto , Ferreira Rita , Amado Francisco
Published: December 2023

The salivary glands play a central role in the secretion of saliva, whose composition and volume affect oral and overall health. A lesser-explored dimension encompasses the possible changes in salivary gland proteomes in response to fluctuations in sex hormone levels. This study aimed to examine the effects of chronic exposure to testosterone on salivary gland remodeling, particularly focusing on proteomic adaptations. Therefore, male Wistar rats were implanted with subcutaneous testosterone-releasing devices at 14 weeks of age. Their submandibular glands were histologically and molecularly analyzed 47 weeks later. The results underscored a significant increase in gland mass after testosterone exposure, further supported by histologic evidence of granular duct enlargement. Despite increased circulating sex hormones, there was no detectable shift in the tissue levels of estrogen alpha and androgen receptors. GeLC-MS/MS and subsequent bioinformatics identified 308 proteins in the submandibular glands, 12 of which were modulated by testosterone. Of note was the pronounced upregulation of Klk3 and the downregulation of Klk6 and Klk7 after testosterone exposure. Protein–protein interaction analysis with the androgen receptor suggests that Klk3 is a potential target of androgenic signaling, paralleling previous findings in the prostate. This exploratory analysis sheds light on the response of salivary glands to testosterone exposure, providing proteome-level insights into the associated weight and histological changes.
Chitosan: Structural and Chemical Modification, Properties, and Application
Authors: Kluczka Joanna
Published: December 2023

Chitosan is a polymer of natural origins that possesses many favourable properties [...]
Ectromelia Virus Affects the Formation and Spatial Organization of Adhesive Structures in Murine Dendritic Cells In Vitro
Authors: Biernacka Zuzanna , Gregorczyk-Zboroch Karolina , Lasocka Iwona , Ostrowska Agnieszka , Struzik Justyna , Gieryńska Małgorzata , Toka Felix N. , Szulc-Dąbrowska Lidia
Published: December 2023

Ectromelia virus (ECTV) is a causative agent of mousepox. It provides a suitable model for studying the immunobiology of orthopoxviruses, including their interaction with the host cell cytoskeleton. As professional antigen-presenting cells, dendritic cells (DCs) control the pericellular environment, capture antigens, and present them to T lymphocytes after migration to secondary lymphoid organs. Migration of immature DCs is possible due to the presence of specialized adhesion structures, such as podosomes or focal adhesions (FAs). Since assembly and disassembly of adhesive structures are highly associated with DCs’ immunoregulatory and migratory functions, we evaluated how ECTV infection targets podosomes and FAs’ organization and formation in natural-host bone marrow-derived DCs (BMDC). We found that ECTV induces a rapid dissolution of podosomes at the early stages of infection, accompanied by the development of larger and wider FAs than in uninfected control cells. At later stages of infection, FAs were predominantly observed in long cellular extensions, formed extensively by infected cells. Dissolution of podosomes in ECTV-infected BMDCs was not associated with maturation and increased 2D cell migration in a wound healing assay; however, accelerated transwell migration of ECTV-infected cells towards supernatants derived from LPS-conditioned BMDCs was observed. We suggest that ECTV-induced changes in the spatial organization of adhesive structures in DCs may alter the adhesiveness/migration of DCs during some conditions, e.g., inflammation.
Modeling User Participation in Facebook Live by Applying the Mediating Role of Social Presence
Authors: Lum Yimin , Chang Chen-Wei
Published: December 2023

The rapid development of mobile Internet technology has brought about the flourishing growth of social media live streaming. This study employs social presence theory as the primary framework to investigate the impact of Facebook Live’s features of sociality, immediacy, and entertainment on users’ sense of presence. These features were then considered within the dimensions of awareness, emotion, and cognition. The influence of social presence on user engagement behaviors was divided into browsing behavior, interactive behavior, and creative behavior. Using snowball sampling, an online survey was administered to Facebook Live users, and 416 valid responses were collected. The research team used software to analyze the data, primarily encompassing descriptive statistics, reliability and validity analyses, structural equation modeling, and mediation effects testing. The research findings are as follows. First, the media characteristics of Facebook Live significantly influence the sense of presence. Specifically, sociality, immediacy, and entertainment on Facebook Live have a notable impact on users’ awareness, emotion, and cognition. Second, different dimensions of social presence have distinct effects on various user engagement behaviors. Notably, the dimensions of awareness, emotion, and cognition of social presence positively affect users’ browsing and interactive behaviors, while emotion influences users’ creative behavior. The third finding was that awareness, emotion, and cognition act as intermediates between Facebook Live’s media characteristics and user engagement behaviors. Implications are discussed.
Fabrication of a Laminated Actuator with Excellent Linearity Using Ground Potassium Sodium Niobate-Based Ceramic Sheets
Authors: Zhang Youming , Hang Qiang , Zheng Dongxi , Lin Fei , Chen Caifeng
Published: December 2023

Linearity is an important factor that affects actuator accuracy. However, the high nonlinearity of KNN piezoelectric ceramics restricts their application in actuators. In this study, we used grinding stress to improve the linearity of ceramic chips, and used them to fabricate a laminated actuator. The ceramic sheets were ground to a thickness of 0.5 mm. During grinding, some areas of the ceramic changed from tetragonal to orthorhombic, owing to the grinding stress. The piezoelectric constant (d33) increased from 198 to 268 pC/N. Notably, the linearity of the ceramics improved. Seven pieces of ground ceramics were bound, to fabricate a laminated multilayer actuator with a total thickness of 3.5 mm. A DC voltage was applied to the actuator, and the displacement was measured. The displacement reached 0.73 μm under a low driving voltage of 200 V. A linear regression analysis of the displacement–voltage relationship was performed, obtaining the regression equation of the actuator. The linearity correlation coefficient was approximately 0.9903, implying that the actuator exhibits a high accuracy. The grinding stress improved the linearity, together with the piezoelectric properties of the ceramic chips, thus improving the actuator accuracy. This research will promote the application of KNN piezoelectric ceramics in actuators.
Radio Telemetry and Harmonic Radar Tracking of the Spotted Lanternfly, Lycorma delicatula (White) (Hemiptera: Fulgoridae)
Authors: Siderhurst Matthew S. , Murman Kelly M. , Kaye Kyle T. , Wallace Matthew S. , Cooperband Miriam F.
Published: December 2023

Lycorma delicatula (White) (Hemiptera: Fulgoridae), spotted lanternfly (SLF), is an invasive pest that feeds and oviposits on numerous woody and herbaceous plants important to agricultural, forest, ornamental, and nursery industries. Describing and understanding SLF movements is key to implementing surveillance and control strategies for this pest and projecting population spread. We used radio telemetry (RT) and harmonic radar (HR) to track the movements of individual SLF at field sites in eastern Pennsylvania and northwestern New Jersey. SLF equipped with HR or RT tags were tracked in 2019 and 2020 from adult emergence until oviposition time, and their movements are described. Although the bulkier RT tags disproportionately affected the distance traveled by males, which are smaller than females, both males and females were more likely to be lost due to signal attenuation when affixed with the lighter-weight HR tags. Females were tracked moving longer distances than males, with maximum distances of 434 m by a single female and 57 m by a single male. A significant positive relationship was found between their height in trees and the distance of subsequent movement. Adult SLF were found in trees predominantly at heights between 6–9 m high. For the fraction of SLF found at eye level, males, but not females, significantly moved above eye level in the weeks prior to mating, likely resulting in the observed sex ratio shift that defines the Early-2 stage. During mating time, tracked SLF were significantly higher than 8 m and oriented to trees where tight aggregations of SLF were present. This orientation towards tight aggregations started when mating began and peaked in the following 2.5 weeks for males in Late-1 and the beginning of Late-2 (after oviposition began), whereas females started this orientation behavior a half-week after males, and this activity peaked for two weeks. Male and female SLF adults exhibited slight differences in host preference, and strong preferences for wild grape, black walnut, sweet birch, and tree-of-heaven were observed. The HR-tagged nymphs moved up to 27.6 m over a five-day period in a cornfield. Nitinol wire HR tags performed better than Wollaston process or tungsten wire tags. SLF movement parameters in the field are described.
A Distributed Lightweight PUF-Based Mutual Authentication Protocol for IoV
Authors: Alkanhal Mona , Alali Abdulaziz , Younis Mohamed
Published: December 2023

In recent times, the advent of innovative technological paradigms like the Internet of Things has paved the way for numerous applications that enhance the quality of human life. A remarkable application of IoT that has emerged is the Internet of Vehicles (IoV), motivated by an unparalleled surge of connected vehicles on the roads. IoV has become an area of significant interest due to its potential in enhancing traffic safety as well as providing accurate routing information. The primary objective of IoV is to maintain strict latency standards while ensuring confidentiality and security. Given the high mobility and limited bandwidth, vehicles need to have rapid and frequent authentication. Securing Vehicle-to-Roadside unit (V2R) and Vehicle-to-Vehicle (V2V) communications in IoV is essential for preventing critical information leakage to an adversary or unauthenticated users. To address these challenges, this paper proposes a novel mutual authentication protocol which incorporates hardware-based security primitives, namely physically unclonable functions (PUFs) with Multi-Input Multi-Output (MIMO) physical layer communications. The protocol allows a V2V and V2R to mutually authenticate each other without the involvement of a trusted third-party (server). The protocol design effectively mitigates modeling attacks and impersonation attempts, where the accuracy of predicting the value of each PUF response bit does not exceed 54%, which is equivalent to a random guess.
Myocardial Calcium Handling in Type 2 Diabetes: A Novel Therapeutic Target
Authors: Dattani Abhishek , Singh Anvesha , McCann Gerry P. , Gulsin Gaurav S.
Published: December 2023

Type 2 diabetes (T2D) is a multisystem disease with rapidly increasing global prevalence. Heart failure has emerged as a major complication of T2D. Dysregulated myocardial calcium handling is evident in the failing heart and this may be a key driver of cardiomyopathy in T2D, but until recently this has only been demonstrated in animal models. In this review, we describe the physiological concepts behind calcium handling within the cardiomyocyte and the application of novel imaging techniques for the quantification of myocardial calcium uptake. We take an in-depth look at the evidence for the impairment of calcium handling in T2D using pre-clinical models as well as in vivo studies, following which we discuss potential novel therapeutic approaches targeting dysregulated myocardial calcium handling in T2D.
Multimodal Cardiac Imaging in the Assessment of Patients Who Have Suffered a Cardioembolic Stroke: A Review
Authors: Thong Elizabeth Hui En , Kong William K. F. , Poh Kian-Keong , Wong Raymond , Chai Ping , Sia Ching-Hui
Published: December 2023

Cardioembolic strokes account for 20–25% of all ischaemic strokes, with their incidence increasing with age. Cardiac imaging plays a crucial role in identifying cardioembolic causes of stroke, with early and accurate identification affecting treatment, preventing recurrence, and reducing stroke incidence. Echocardiography serves as the mainstay of cardiac evaluation. Transthoracic echocardiography (TTE) is the first line in the basic evaluation of structural heart disorders, valvular disease, vegetations, and intraventricular thrombus. It can be used to measure chamber size and systolic/diastolic function. Trans-oesophageal echocardiography (TOE) yields better results in identifying potential cardioembolic sources of stroke and should be strongly considered, especially if TTE does not yield adequate results. Cardiac computed tomography and cardiac magnetic resonance imaging provide better soft tissue characterisation, high-grade anatomical information, spatial and temporal visualisation, and image reconstruction in multiple planes, especially with contrast. These techniques are useful in cases of inconclusive echocardiograms and can be used to detect and characterise valvular lesions, thrombi, fibrosis, cardiomyopathies, and aortic plaques. Nuclear imaging is not routinely used, but it can be used to assess left-ventricular perfusion, function, and dimensions and may be useful in cases of infective endocarditis. Its use should be considered on a case-by-case basis. The accuracy of each imaging modality depends on the likely source of cardioembolism, and the choice of imaging approach should be tailored to individual patients.
Validation of Quantitative Flow Ratio-Derived Virtual Angioplasty with Post-Angioplasty Fractional Flow Reserve—The QIMERA-I Study
Authors: Amat-Santos Ignacio J. , Marengo Giorgio , Sánchez-Luna Juan Pablo , Cortés Villar Carlos , Rivero Crespo Fernando , Jiménez Díaz Víctor Alfonso , de la Torre Hernández José María , Pérez de Prado Armando , Sabaté Manel , López-Palop Ramón , Vegas Valle José Miguel , Suárez de Lezo Javier , Fernandez Cordon Clara , Gonzalez Jose Carlos , García-Gómez Mario , Redondo Alfredo , Carrasco Moraleja Manuel , San Román J. Alberto
Published: December 2023

Background: Quantitative flow ratio (QFR) virtual angioplasty with pre-PCI residual QFR showed better results compared with an angiographic approach to assess post-PCI functional results. However, correlation with pre-PCI residual QFR and post-PCI fractional flow reserve (FFR) is lacking. Methods: A multicenter prospective study including consecutive patients with angiographically 50–90% coronary lesions and positive QFR results. All patients were evaluated with QFR, hyperemic and non-hyperemic pressure ratios (NHPR) before and after the index PCI. Pre-PCI residual QFR (virtual angioplasty) was calculated and compared with post-PCI fractional flow reserve (FFR), QFR and NHPR. Results: A total of 84 patients with 92 treated coronary lesions were included, with a mean age of 65.5 ± 10.9 years and 59% of single vessel lesions being the left anterior descending artery in 69%. The mean vessel diameter was 2.82 ± 0.41 mm. Procedural success was achieved in all cases, with a mean number of implanted stents of 1.17 ± 0.46. The baseline QFR value was 0.69 ± 0.12 and baseline FFR and NHPR were 0.73 ± 0.08 and 0.82 ± 0.11, respectively. Mean post-PCI FFR increased to 0.87 ± 0.05 whereas residual QFR had been estimated as 0.95 ± 0.05, showing poor correlation with post-PCI FFR (0.163; 95% CI:0.078–0.386) and low diagnostic accuracy (30.9%, 95% CI:20–43%). Conclusions: In this analysis, the results of QFR-based virtual angioplasty did not seem to accurately correlate with post-PCI FFR.
MTHFR Gene C677T Polymorphism (rs1801133) and Susceptibility to Colorectal Polyps in an Azerbaijani Population
Authors: Aslanov Hazi , Bayramov Bayram , Reissfelder Christoph , Abdullayeva Shams , Mammadova Zeynab , Aliyev Fikrat , Keese Michael , Hajibabazade Javahir , Yagublu Vugar
Published: December 2023

Background: Understanding the relationships between the methylenetetrahydrofolate reductase (MTHFR) gene polymorphism, colorectal polyps, and CRC risk can aid in advancing personalized medicine approaches in CRC prevention. The aim of the current study is to identify the association of C677T polymorphism of the MTHFR gene with the risk of colorectal polyps in the Azerbaijani population. Methods: This study included 125 patients with colon polyps and 155 healthy individuals as a control group. DNA was extracted from venous blood samples obtained from patients and healthy individuals, and the results were analyzed through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and agarose gel electrophoresis. Results: Wild-type, heterozygote, and homozygous mutant were revealed within 69 (55.2%), 49 (39.2%), and 7 (5.6%) patients and within 100 (64.5%), 45 (29%), and 10 (6.5%) healthy controls, respectively. However, no significant statistical associations were observed between CT and TT genotypes, dominant (CC vs. CT + TT) and recessive (CC + CT vs. TT) models, and the mutant T allele and disease risk. There were also no significant differences between patients and controls regarding age, sex, smoking and alcohol use. Conclusion: Our research did not reveal any significant association between the MTHFR C677T polymorphism and susceptibility to colorectal polyps in the Azerbaijan population.
Cardiac Amyloidosis and Valvular Heart Disease
Authors: Duca Franz , Kronberger Christina , Willixhofer Robin , Bartko Philipp E. , Bergler-Klein Jutta , Nitsche Christian
Published: December 2023

Growing interest has accrued in the co-existence of cardiac amyloidosis and valvular heart disease. Amyloid infiltration from either transthyretin (ATTR) or of light chain (AL) origin may affect any structure of the heart, including the valves. The recent literature has mainly focused on aortic stenosis and cardiac amyloidosis, improving our understanding of the epidemiology, diagnosis, treatment and prognosis of this dual pathology. Despite being of high clinical relevance, data on mitral/tricuspid regurgitation and cardiac amyloidosis are rather scarce and mostly limited to case reports and small cases series. It is the aim of this review article to summarize the current evidence of concomitant valvular heart disease and cardiac amyloidosis by including studies on epidemiology, diagnostic approaches, screening possibilities, therapeutic management, and prognostic implications.
Dipole Density Guided Catheter Ablation versus Conventional Substrate Modification for Repeat Catheter Ablation of Persistent Atrial Fibrillation
Authors: Schipper Jan-Hendrik , Steven Daniel , Lüker Jakob , Wörmann Jonas , van den Bruck Jan-Hendrik , Filipovic Karlo , Dittrich Sebastian , Scheurlen Cornelia , Erlhöfer Susanne , Pavel Friederike , Sultan Arian
Published: December 2023

Aims: The optimal ablation strategy for recurrent persistent atrial fibrillation (persAF) after initially successful catheter ablation (CA) remains debatable. Dipole density (DD) guided CA using the AcQMap system has been proven to be feasible and effective in patients with persAF. So far, long-term outcome data for DD-guided CA in patients with recurrence of persAF are sparse. This study sought to assess long-term outcome data in patients undergoing a DD-guided CA for recurrence of persAF after previous CA in comparison to conventional repeat CA. Methods and Results: Patients undergoing DD-guided CA for recurrence of persAF after previous ablation were compared to patients undergoing conventional substrate modification (CSM). A total of 64 patients (32 DD-guided and 32 CSM) were included in this analysis. Procedure duration (DD: 236 ± 61 min; CSM: 198 ± 59 min; p = 0.004) and fluoroscopy time (DD: 36 ± 15 min; CSM: 20 ± 11 min; p = 0.0001) were significantly longer in the DD group. After a long-term median follow-up (FU) of 27 months (interquartile range 12.8–34.3), DD-guided CA was inferior to CSM regarding overall arrhythmia-free survival (DD: 6 patients (19%), CSM: 11 patients (34%); HR 1.47; p = 0.04). Freedom from AF did not differ between both groups (DD: 16 patients (50%); CSM: 18 patients (56%), HR 0.99, p = 0.47). During FU, more patients underwent repeat CA after DD-guided ablation (DD: 16 patients (50%), CSM: 7 patients (22%), p = 0.04). No major complications occurred overall. Conclusions: Dipole density-guided CA is equally safe but associated with longer procedure duration compared to conventional substrate modification for treatment of recurrent persAF after previous CA. Of note, long-term arrhythmia-free survival is significantly worse after DD-guided ablation, and more patients undergo redo procedures.
Esophageal Endoscopic Submucosal Dissection in Older Patients Is Safe and Feasible: A Retrospective Single-Center Cohort Study in the United States
Authors: Koseki Mako , Nishimura Makoto , Beauvais Jacques C. , Nammour Tarek , Nagao Sayaka , Schattner Mark A.
Published: December 2023

Background: Endoscopic submucosal dissection (ESD) is a well–established method for treating early esophageal carcinomas. However, data on the safety and efficacy of esophageal ESD in older patients in the United States are limited. Methods: This retrospective study investigated the outcomes of esophageal ESD in patients aged ≥80 years and included those who underwent esophageal ESD between June 2018 and April 2023 at a single center in the United States. Patients were divided into two age groups for comparison: ≥80 and <80 years. Treatment outcomes and complications were evaluated and compared between these groups. Results: A total of 53 cases of esophageal ESD for malignant neoplasms were included, with 12 patients in the ≥80 years age group. No significant differences were observed in the patients’ background and characteristics, except for a prior history of interventions (p = 0.04). The en bloc resection rate was 100% in both groups. The R0 resection rate was lower in the ≥80 years age group (75% vs. 88%). There were no complications requiring additional intervention in the ≥80 years age group, such as post–ESD bleeding, perforation, mediastinal emphysema, or pneumonia. Conclusions: Esophageal ESD may be a safe and feasible procedure for treating esophageal carcinomas in older patients.