The Tabula Sapiens: A multiple-organ, single-cell transcriptomic atlas of humans
Authors: The Tabula Sapiens Consortium*  , Jones Robert C. , Karkanias Jim , Krasnow Mark A. , Pisco Angela Oliveira , Quake Stephen R. , Salzman Julia , Yosef Nir , Bulthaup Bryan , Brown Phillip , Harper William , Hemenez Marisa , Ponnusamy Ravikumar , Salehi Ahmad , Sanagavarapu Bhavani A. , Spallino Eileen , Aaron Ksenia A. , Concepcion Waldo , Gardner James M. , Kelly Burnett , Neidlinger Nikole , Wang Zifa , Crasta Sheela , Kolluru Saroja , Morri Maurizio , Tan Serena Y. , Travaglini Kyle J. , Xu Chenling , Alcántara-Hernández Marcela , Almanzar Nicole , Antony Jane , Beyersdorf Benjamin , Burhan Deviana , Calcuttawala Kruti , Carter Matthew M. , Chan Charles K. F. , Chang Charles A. , Chang Stephen , Colville Alex , Culver Rebecca N. , Cvijović Ivana , D’Amato Gaetano , Ezran Camille , Galdos Francisco X. , Gillich Astrid , Goodyer William R. , Hang Yan , Hayashi Alyssa , Houshdaran Sahar , Huang Xianxi , Irwin Juan C. , Jang SoRi , Juanico Julia Vallve , Kershner Aaron M. , Kim Soochi , Kiss Bernhard , Kong William , Kumar Maya E. , Kuo Angera H. , Leylek Rebecca , Li Baoxiang , Loeb Gabriel B. , Lu Wan-Jin , Mantri Sruthi , Markovic Maxim , McAlpine Patrick L. , de Morree Antoine , Mrouj Karim , Mukherjee Shravani , Muser Tyler , Neuhöfer Patrick , Nguyen Thi D. , Perez Kimberly , Phansalkar Ragini , Puluca Nazan , Qi Zhen , Rao Poorvi , Raquer-McKay Hayley , Schaum Nicholas , Scott Bronwyn , Seddighzadeh Bobak , Segal Joe , Sen Sushmita , Sikandar Shaheen , Spencer Sean P. , Steffes Lea C. , Subramaniam Varun R. , Swarup Aditi , Swift Michael , Van Treuren Will , Trimm Emily , Veizades Stefan , Vijayakumar Sivakamasundari , Vo Kim Chi , Vorperian Sevahn K. , Wang Wanxin , Weinstein Hannah N. W. , Winkler Juliane , Wu Timothy T. H. , Xie Jamie , Yung Andrea R. , Zhang Yue , Detweiler Angela M. , Mekonen Honey , Neff Norma F. , Sit Rene V. , Tan Michelle , Yan Jia , Bean Gregory R. , Charu Vivek , Forgó Erna , Martin Brock A. , Ozawa Michael G. , Silva Oscar , Toland Angus , Vemuri Venkata N. P. , Afik Shaked , Awayan Kyle , Botvinnik Olga Borisovna , Byrne Ashley , Chen Michelle , Dehghannasiri Roozbeh , Gayoso Adam , Granados Alejandro A. , Li Qiqing , Mahmoudabadi Gita , McGeever Aaron , Olivieri Julia Eve , Park Madeline , Ravikumar Neha , Stanley Geoff , Tan Weilun , Tarashansky Alexander J. , Vanheusden Rohan , Wang Peter , Wang Sheng , Xing Galen , Dethlefsen Les , Ho Po-Yi , Liu Shixuan , Maltzman Jonathan S. , Metzger Ross J. , Sasagawa Koki , Sinha Rahul , Song Hanbing , Wang Bruce , Artandi Steven E. , Beachy Philip A. , Clarke Michael F. , Giudice Linda C. , Huang Franklin W. , Huang Kerwyn Casey , Idoyaga Juliana , Kim Seung K. , Kuo Christin S. , Nguyen Patricia , Rando Thomas A. , Red-Horse Kristy , Reiter Jeremy , Relman David A. , Sonnenburg Justin L. , Wu Albert , Wu Sean M. , Wyss-Coray Tony 
Published: May 2022

Molecular characterization of cell types using single-cell transcriptome sequencing is revolutionizing cell biology and enabling new insights into the physiology of human organs. We created a human reference atlas comprising nearly 500,000 cells from 24 different tissues and organs, many from the same donor. This atlas enabled molecular characterization of more than 400 cell types, their distribution across tissues, and tissue-specific variation in gene expression. Using multiple tissues from a single donor enabled identification of the clonal distribution of T cells between tissues, identification of the tissue-specific mutation rate in B cells, and analysis of the cell cycle state and proliferative potential of shared cell types across tissues. Cell type–specific RNA splicing was discovered and analyzed across tissues within an individual.