bluuebunny/crossref_metadata_2025 huggingface dataset:
Structural Lessons From the Mutant Proinsulin Syndrome
Authors: Dhayalan Balamurugan , Chatterjee Deepak , Chen Yen-Shan , Weiss Michael A.
Published: September 2021

Insight into folding mechanisms of proinsulin has been provided by analysis of dominant diabetes-associated mutations in the human insulin gene (INS). Such mutations cause pancreatic β-cell dysfunction due to toxic misfolding of a mutant proinsulin and impairment in trans of wild-type insulin secretion. Anticipated by the “Akita” mouse (a classical model of monogenic diabetes mellitus; DM), this syndrome illustrates the paradigm endoreticulum (ER) stress leading to intracellular proteotoxicity. Diverse clinical mutations directly or indirectly perturb native disulfide pairing leading to protein misfolding and aberrant aggregation. Although most introduce or remove a cysteine (Cys; leading in either case to an unpaired thiol group), non-Cys-related mutations identify key determinants of folding efficiency. Studies of such mutations suggest that the hormone’s evolution has been constrained not only by structure-function relationships, but also by the susceptibility of its single-chain precursor to impaired foldability. An intriguing hypothesis posits that INS overexpression in response to peripheral insulin resistance likewise leads to chronic ER stress and β-cell dysfunction in the natural history of non-syndromic Type 2 DM. Cryptic contributions of conserved residues to folding efficiency, as uncovered by rare genetic variants, define molecular links between biophysical principles and the emerging paradigm of Darwinian medicine: Biosynthesis of proinsulin at the edge of non-foldability provides a key determinant of “diabesity” as a pandemic disease of civilization.
Impact of Sintered Temperature on the Heat and Cation Transport in NaK-BASE Tube
Authors: Guan Ning , Chen Weijian , Gao Yan
Published: September 2021

Alkali metal thermoelectric converter (AMTEC) is a clean energy converter that can be coupled with biomass for power generation. In present research, the transport of heat and cation was investigated in NaK-BASE tubes prepared at different sintered temperatures. The heat conduction and the fractal model were employed to investigate the temperature distributions based on the microstructures of the NaK-BASE tubes sintered at different temperatures, and the transport of Na+ and K+ in NaK-BASE tube was simulated by Poisson-Nernst-Planck multi-ions transport model, and the cation concentrations and surface charge densities were obtained in the NaK-BASE tubes with different temperatures. The results showed that microstructure of the NaK-BASE was related to the sintered temperature, and the microstructure of the NaK-BASE impacted the temperature distribution, the cation concentration and the surface charge density of Na+ and K+ in the NaK-BASE tubes. At the same heat source temperature, the average temperature in the NaK-BASE prepared at high sintered temperature was higher than that prepared at low sintered temperature. In addition, the increase of the average temperature resulted in the increase of the cation concentration and the surface charge density of Na+ and K+ in the NaK-BASE, therefore, the performance of the NaK-AMTEC could be enhanced by increasing the sintered temperature and the average temperature of the NaK-BASE.
Data-Driven Control for Proton Exchange Membrane Fuel Cells: Method and Application
Authors: Li Jiawen , Zhu Kedong , Yu Tao
Published: September 2021

A data-driven optimal control method for an air supply system in proton exchange membrane fuel cells (PEMFCs) is proposed with the aim of improving the PEMFC net output power and operational efficiency. Moreover, a marginal utility-based double-delay deep deterministic policy gradient (MU-4DPG) algorithm is proposed as a an offline tuner for the PID controller. The coefficients of the PID controller are rectified and optimized during training in order to enhance the controller’s performance. The design of the algorithm draws on the concept of marginal effects in Economics, in that the algorithm continuously switches between different forms of exploration noise during training so as to increase the diversity of samples, improve exploration efficiency and avoid Q-value overfitting, and ultimately improve the robustness of the algorithm. As detailed below, the effectiveness of the control method has been experimentally demonstrated.
Control Method for PEMFC Using Improved Deep Deterministic Policy Gradient Algorithm
Authors: Li Jiawen , Li Yaping , Yu Tao
Published: September 2021

A data-driven PEMFC output voltage control method is proposed. Moreover, an Improved deep deterministic policy gradient algorithm is proposed for this method. The algorithm introduces three techniques: Clipped multiple Q-learning, policy delay update, and policy smoothing to improve the robustness of the control policy. In this algorithm, the hydrogen controller is treated as an agent, which is pre-trained to fully interact with the environment and obtain the optimal control policy. The effectiveness of the proposed algorithm is demonstrated experimentally.
Life History Traits, Elasticity Analyses, and Phenotypic Plasticity of Squaliobarbus curriculus in the Pearl River Estuary, China
Authors: Wang Teng , Lin Lin , Liu Yong , Jakovlić Ivan , Li Chun-hou , Xiao Ya-yuan , Wu Peng
Published: September 2021

Squaliobarbus curriculus, commonly known as red-eye fish, is widely distributed in East Asia. It is one of the important aquatic germplasm resource and economic species in the Pearl River. To give suggestions for better protection and management, we investigated its life history and conducted elasticity analyses. Samples (n= 451) were collected between 2016 and 2017 from the western Pearl River estuary. There were no significant differences between the length-weight relationships of females and males (W= 0.00001SL3.121). The von Bertalanffy growth function was Lt= 553.2 [1 − e−0.111(t+1.009)]. The estimated length at 50% sexual maturity for females was 209.6 and 200.0 mm for males, both at 3 years of age. Oocyte size-frequency distribution suggested batch spawning. Fecundity ranged between 9,407 and 175,086 eggs per fish (mean = 51,040, or 143.9 eggs/g of fish weight). To better understand the ecological phenotypic plasticity ofS. curriculuswe conducted meta-analyses on all available life history data for this species. Our results showed that the standard lengths at ages 2 and 3 in the estuary were significantly smaller than in the upper reaches of the Pearl River basin, and there were also obvious differences in fecundity and oocyte size. For more, the standard lengths at ages 2 and 3 were correlated negatively with latitude. Elasticity analysis showed that juveniles’ (aged 1–3) survival had the largest contribution to the population growth rate, which suggests that management efforts should focus on the early life stages.
Assessments of Air Pollution Control Effectiveness Based on a Sharp Regression Discontinuity Design —Evidence From China’s Environmental Big Data
Authors: Wang Ren , Huang Jiaqi , Zhang Lizhi , Xia Yu , Xu Xu , Nong Tongli
Published: September 2021

The Assessment andAppraisal Method for Ecological Construction Targets (the Method) was promulgated in 2016, which provided a concrete instruction for China’s air pollution control and established an explicit standard for reducing air pollutant concentration. This study implements a sharp regression discontinuity (RD) design and makes an assessment on air quality control effectiveness of the Method based on the high-volume big data acquired from 173 cities in China. The results show that the Method has significantly improved air pollution control on the overall air quality index (AQI) and reducing concentrations of PM2.5, PM10, SO2, NO2, and CO across the country in the observation periods. However, no reduction effect was observed for O3. The robustness tests support the conclusion as well. Besides, the heterogeneity analysis illustrates that the policy had a significant short-term treatment effect in East, South, Central, North, Northwest, Southwest, and Northeast China. However, the Method’s effect is found to decline over time either nationwide or regionally according to the persistence analysis. Therefore, this article puts forward several suggestions regarding the formulation of long-term regulations for air pollution control, the transformation of the growth model for sustainable development, and optimization of the incentive system for improved pollution control and prevention.
Intracellular Storage Reduces Stoichiometric Imbalances in Soil Microbial Biomass – A Theoretical Exploration
Authors: Manzoni Stefano , Ding Yang , Warren Charles , Banfield Callum C. , Dippold Michaela A. , Mason-Jones Kyle
Published: September 2021

Microbial intracellular storage is key to defining microbial resource use strategies and could contribute to carbon (C) and nutrient cycling. However, little attention has been devoted to the role of intracellular storage in soil processes, in particular from a theoretical perspective. Here we fill this gap by integrating intracellular storage dynamics into a microbially explicit soil C and nutrient cycling model. Two ecologically relevant modes of storage are considered: reserve storage, in which elements are routed to a storage compartment in proportion to their uptake rate, and surplus storage, in which elements in excess of microbial stoichiometric requirements are stored and limiting elements are remobilized from storage to fuel growth and microbial maintenance. Our aim is to explore with this model how these different storage modes affect the retention of C and nutrients in active microbial biomass under idealized conditions mimicking a substrate pulse experiment. As a case study, we describe C and phosphorus (P) dynamics using literature data to estimate model parameters. Both storage modes enhance the retention of elements in microbial biomass, but the surplus storage mode is more effective to selectively store or remobilize C and nutrients according to microbial needs. Enhancement of microbial growth by both storage modes is largest when the substrate C:nutrient ratio is high (causing nutrient limitation after substrate addition) and the amount of added substrate is large. Moreover, storage increases biomass nutrient retention and growth more effectively when resources are supplied in a few large pulses compared to several smaller pulses (mimicking a nearly constant supply), which suggests storage to be particularly relevant in highly dynamic soil microhabitats. Overall, our results indicate that storage dynamics are most important under conditions of strong stoichiometric imbalance and may be of high ecological relevance in soil environments experiencing large variations in C and nutrient supply.
Hormonal Regulation of Diapause and Development in Nematodes, Insects, and Fishes
Authors: Karp Xantha
Published: September 2021

Diapause is a state of developmental arrest adopted in response to or in anticipation of environmental conditions that are unfavorable for growth. In many cases, diapause is facultative, such that animals may undergo either a diapause or a non-diapause developmental trajectory, depending on environmental cues. Diapause is characterized by enhanced stress resistance, reduced metabolism, and increased longevity. The ability to postpone reproduction until suitable conditions are found is important to the survival of many animals, and both vertebrate and invertebrate species can undergo diapause. The decision to enter diapause occurs at the level of the whole animal, and thus hormonal signaling pathways are common regulators of the diapause decision. Unlike other types of developmental arrest, diapause is programmed, such that the diapause developmental trajectory includes a pre-diapause preparatory phase, diapause itself, recovery from diapause, and post-diapause development. Therefore, developmental pathways are profoundly affected by diapause. Here, I review two conserved hormonal pathways, insulin/IGF signaling (IIS) and nuclear hormone receptor signaling (NHR), and their role in regulating diapause across three animal phyla. Specifically, the species reviewed are Austrofundulus limnaeus and Nothobranchius furzeri annual killifishes, Caenorhabditis elegans nematodes, and insect species including Drosophila melanogaster, Culex pipiens, and Bombyx mori. In addition, the developmental changes that occur as a result of diapause are discussed, with a focus on how IIS and NHR pathways interact with core developmental pathways in C. elegans larvae that undergo diapause.
Canopy Position Influences the Degree of Light Suppression of Leaf Respiration in Abundant Tree Genera in the Amazon Forest
Authors: Souza Daisy C. , Jardine Kolby J. , Rodrigues João V. F. C. , Gimenez Bruno O. , Rogers Alistair , McDowell Nate , Walker Anthony P. , Higuchi Niro , Sampaio-Filho Israel J. , Chambers Jeffrey
Published: September 2021

Leaf respiration in the dark (Rdark) and light (Rday) is poorly characterized in diverse tropical ecosystems, and little to no information exists on the degree of light suppression in common tree species within the Amazon basin, and their dependences upon plant functional traits and position within the canopy. We quantified Rdark and apparent Rday using the Kok method and measured key leaf traits in 26 tree individuals of different species distributed in three different canopy positions: canopy, lower canopy, and understory. To explore the relationships between the leaf traits we used the standardized major axis (SMA). We found that canopy trees had significantly higher rates of Rdark and Rday than trees in the understory. The difference between Rdark and Rday (the light suppression of respiration) was greatest in the understory (68 ± 9%, 95% CI) and lower canopy (49 ± 9%, 95% CI) when compared to the canopy (37 ± 10%, 95% CI). We also found that Rday was significantly and strongly correlated with Rdark (p < 0.001) for all the canopy positions. Also, leaf mass per area (LMA) and leaf Phosphorus concentration (P) had a significant relationship with Rdark (p < 0.001; p = 0.003), respectively. In addition, a significant relationship was found for LMA in the canopy and lower canopy positions (p = 0.009; p = 0.048) while P was only significant in the canopy (p = 0.044). Finally, no significant relationship was found between Rdark and nitrogen, sugars, and starch. Our results highlight the importance of including representation of the light suppression of leaf respiration in terrestrial biosphere models and also of accounting for vertical gradients within forest canopies and connections with functional traits.
Twenty-Seven Y-Chromosome Short Tandem Repeats Analysis of Italian Mummies of the 16th and 18th Centuries: An Interdisciplinary Research
Authors: Bini Carla , Cilli Elisabetta , Sarno Stefania , Traversari Mirko , Fontani Francesco , Boattini Alessio , Pelotti Susi , Luiselli Donata
Published: September 2021

Roccapelago (MO) is a small village located in the Northern Central Apennines, with a population of 31 inhabitants (2014). In 2010, more than 400 individuals dated between the end of the 16th and the 18th century, many of which partially mummified, were discovered in the crypt of the church. This small village, because of its geographical location and surrounding environment, seems to possess the characteristics of a genetic isolate, useful for population genetics and genealogical analyses. Thus, a diachronic study of DNA aimed at investigating the structure and dynamics of the population of Roccapelago over the about 4 centuries, was conducted by analyzing ancient and modern inhabitants of the village. The 14 modern samples were selected by considering both the founder surnames of the village, identified thanks to the study of parish registers, and the grandparent’s criterion. From 25 ancient mummies, morphologically assigned to male individuals, the petrous bone, that harbors high DNA amounts, was selected for the DNA extraction. The quantification and qualitative assessment of total human male DNA were evaluated by a real-time PCR assay using the Quantifiler Trio DNA Quantification Kit and multiplex PCR of 27 Y-chromosome short tandem repeat (Y-STR) markers included in the Yfiler Plus PCR Amplification Kit, with seven rapidly mutating Y-STR loci for improving discrimination of male lineages, was performed to genotype the samples. Y-STRs were analyzed according to the criteria of ancient DNA (aDNA) analysis to ensure that authentic DNA typing results were obtained from these ancient samples. The molecular analysis showed the usefulness of the Y chromosome to identify historically relevant remains and discover patterns of relatedness in communities moving from anthropology to genetic genealogy and forensics.
The Multi-Omic Prognostic Model of Oxidative Stress-Related Genes in Acute Myeloid Leukemia
Authors: Dong Chao , Zhang Naijin , Zhang Lijun
Published: September 2021

Background: Acute myeloid leukemia (AML) is one of the most common cancers in the world, and oxidative stress is closely related to leukemia. A lot of effort has been made to improve the prognosis of AML. However, the situation remains serious. Hence, we focused on the study of prognostic genes in AML.

Materials and Methods: Prognostic oxidative stress genes were screened out. The gene expression profile of AML patients was downloaded from the The Cancer Genome Atlas (TCGA) database. The oxidative stress-related model was constructed, by which the prognosis of AML patients was predicted using the two GEO GSE23143 datasets and the stability of the GSE71014 authentication model.

Results: The prognostic oxidative stress genes were screened out in AML, and the prognostic genes were significantly enriched in a large number of pathways based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. There was a complex interaction between prognostic genes and transcription factors. After constructing the prediction model, the clinical predictive value of the model was discussed in a multi-omic study. We investigated the sensitivity of risk score to common chemotherapeutic agents, the influence of signaling pathways on the prognosis of AML patients, and the correlation of multiple genes with immune score and immune dysfunction.

Conclusions: A highly effective prognostic risk model for AML patients was established and validated. The association of prognostic oxidative stress genes with drug sensitivity, signaling pathways, and immune infiltration was explored. The results suggested that oxidative stress genes promised to be potential prognostic biomarkers for AML, which may provide a new basis for disease management.
Whole-Genome Sequencing and Genome-Wide Studies of Spiny Head Croaker (Collichthys lucidus) Reveals Potential Insights for Well-Developed Otoliths in the Family Sciaenidae
Authors: Gan Wu , Zhao Chenxi , Liu Xinran , Bian Chao , Shi Qiong , You Xinxin , Song Wei
Published: September 2021

Spiny head croaker (Collichthys lucidus), belonging to the family Sciaenidae, is a small economic fish with a main distribution in the coastal waters of Northwestern Pacific. Here, we constructed a nonredundant chromosome-level genome assembly of spiny head croaker and also made genome-wide investigations on genome evolution and gene families related to otolith development. A primary genome assembly of 811.23 Mb, with a contig N50 of 74.92 kb, was generated by a combination of 49.12-Gb Illumina clean reads and 35.24 Gb of PacBio long reads. Contigs of this draft assembly were further anchored into chromosomes by integration with additional 185.33-Gb Hi-C data, resulting in a high-quality chromosome-level genome assembly of 817.24 Mb, with an improved scaffold N50 of 26.58 Mb. Based on our phylogenetic analysis, we observed that C. lucidus is much closer to Larimichthys crocea than Miichthys miiuy. We also predicted that many gene families were significantly expanded (p-value <0.05) in spiny head croaker; among them, some are associated with “calcium signaling pathway” and potential “inner ear functions.” In addition, we identified some otolith-related genes (such as otol1a that encodes Otolin-1a) with critical deletions or mutations, suggesting possible molecular mechanisms for well-developed otoliths in the family Sciaenidae.
Mesenchymal Stem Cell Therapy: Hope for Patients With Systemic Lupus Erythematosus
Authors: Li Aifen , Guo Fengbiao , Pan Quanren , Chen Shuxian , Chen Jiaxuan , Liu Hua-feng , Pan Qingjun
Published: September 2021

Systemic lupus erythematosus (SLE) is a chronic autoimmune disease. Although previous studies have demonstrated that SLE is related to the imbalance of cells in the immune system, including B cells, T cells, and dendritic cells, etc., the mechanisms underlying SLE pathogenesis remain unclear. Therefore, effective and low side-effect therapies for SLE are lacking. Recently, mesenchymal stem cell (MSC) therapy for autoimmune diseases, particularly SLE, has gained increasing attention. This therapy can improve the signs and symptoms of refractory SLE by promoting the proliferation of Th2 and Treg cells and inhibiting the activity of Th1, Th17, and B cells, etc. However, MSC therapy is also reported ineffective in some patients with SLE, which may be related to MSC- or patient-derived factors. Therefore, the therapeutic effects of MSCs should be further confirmed. This review summarizes the status of MSC therapy in refractory SLE treatment and potential reasons for the ineffectiveness of MSC therapy from three perspectives. We propose various MSC modification methods that may be beneficial in enhancing the immunosuppression of MSCs in SLE. However, their safety and protective effects in patients with SLE still need to be confirmed by further experimental and clinical evidence.
Stock Assessment of Hatchery-Released Clam Meretrix meretrix in an Estuary of China From the Perspectives of Population Ecology and Genetic Diversity
Authors: Zhang Anguo , Li Hongjun , Yang Xiaolong , Wang Lili , Gao Yangchun , Song Mingshan , Yuan Xiutang
Published: September 2021

In this study, the release effects of the buried clamMeretrix meretrixin a northern estuary of China (Shuangtaizi Estuary) was assessed in terms of population ecology and genetic variability by using longer-term monitoring data from 2011 (used as the baseline data) to 2018. A total of 2.4 × 108hatchery juveniles were released from 2012 to 2014, and follow-up monitoring survey of the growth, distribution, density, and biomass ofM. meretrixpopulation was carried out. Results showed a temporary spike in the population ofM. meretrix, and the population underwent considerable year-to-year fluctuations in total abundance and biomass. Meanwhile, the age structure of the populations from 2011 to 2018 is still an unstable expanding type. Genetic analysis based on 10 polymorphic microsatellite markers revealed no significant genetic differentiation and weak genetic structures between years but higher genetic diversities in 2014–2018 than in the initial period (2013) of stock restoration. Our study suggests that effective policies for management and conservation to restoreM. meretrixpopulation by local governments are still needed in the long run.
Introducing Progressive Strength Training Program in Singapore's Elder Care Settings
Authors: Björkgren Magnus , Borg Frank , Tan Ken , Laxåback Gerd , Nygård Lisette
Published: September 2021

The use of progressive strength training among the elderly has become an accepted part of evidence-based practice for preventive and rehabilitative care. Exercise is undoubtedly one of the pillars for resilient aging. While research has shown impressive outcomes from strength training, the challenge remains to get elderly persons to exercise. Here we describe a Finnish-Singaporean cross-national project that provides a unique opportunity to evaluate the implementation of strength training in settings where it had previously not been applied. We report from the first 2 years of implementation using assessment data and surveys directed to frontline therapists responsible for the implementation. The strength training concept was progressively implemented in 24 elder care locations in Singapore including residential homes, day rehab/care centers, and senior activity centers. Each location was provided with training, support, gym equipment and technology solutions. It remained for individual sites to enroll elderly to the program, to perform assessments, and to direct the progressive strength training. Based on data from the first 2 years of implementation, improvements in lower body muscle strength were found in Leg Curl (ave 11.1–48.8%), Leg Extension (ave 10.2–24.0%) and Hip Abduction/Hip Adduction (ave 7.0–15.8%). Of the trained therapists, 95% strongly agreed or agreed to some extent that the implementation had been successful. The practice-based evidence from the project has demonstrated that it is feasible to implement progressive strength training in real life settings, using technology. While the implementation initially required handholding and support, the approach yielded consistent improvement rates in muscle strength comparable to results from randomized clinical trials (meta-analysis studies). Significant improvement rates in muscle strength were found in all three types of sites, demonstrating that gym training can be employed broadly in elder care. The Senior Activity Centers offer an interesting model for reaching seniors with preventive actions at an early stage. The data support a 3-month training as an effective intervention of introducing strength training in elder care settings, promoting healthy aging.
Mycoherbicides for the Noxious Meddlesome: Can Colletotrichum be a Budding Candidate?
Authors: Chakraborty Anwesha , Ray Puja
Published: September 2021

Invasive plant species are a major threat to biodiversity and agricultural productivity. Hence, efforts to manage these menace involves extensive and effective use of chemical herbicides amongst others. However, not only is the impact of control with chemical herbicides short-lived but also leads to negative impact on human health and environment due to non-target herbicide-drift and runoff from the sprayed areas. This has ushed in much-anticipated nature-based potential regulators of weed species, in an attempt to lower the utilisation of chemical herbicides. Mycoherbicides have been seen as a benign, eco-friendly, host-specific, and replacement for chemical herbicides. There are several noteworthy genera of fungus that have been proved to be effective against weeds. They either produce strong phytotoxins or are often used as spore/conidia-based solutions and applied as a spray in growth media. One of such potential genera is Colletotrichum Corda 1831. Compared to other potent fungal genera, with well-established roles in conferring herbicidal activities by producing competent phytotoxins, only a few species under genus Colletotrichum are known to produce fungal metabolites be used as phytotoxins. This article elucidates the current understanding of using spore suspension/phytotoxin of Colletotrichum as a weedicide. We also discuss the interaction between fungal metabolites release and Colletotrichum-target plant, from a molecular and biochemical point of view. This review article has been written to accentuate on the potency of Colletotrichum, and to serve as an eye-opener to consider this genus for further fruitful investigations. However, inconsistency associated with mycoherbicides in terms of viability and efficacy under field conditions, production of bioactive compound, slow natural dispersal ability, etc., have often reduced their utility. Hence, our study emphasizes on the need to do extensive research in elucidating more phytotoxins from necrotrophic phytopathogenic microorganisms with novel mode of action for field application.
A Comparative Analysis of the Microbiome of Kiwifruit at Harvest Under Open-Field and Rain-Shelter Cultivation Systems
Authors: Sui Yuan , Zhao Qianhua , Wang Zhenshuo , Liu Jia , Jiang Mingguo , Yue Junyang , Lan Jianbin , Liu Jing , Liao Qinhong , Wang Qi , Yang Qiya , Zhang Hongyin
Published: September 2021

The composition of microbial communities can directly affect fruit quality, health status, and storability. The present study characterized the epiphytes and endophytes of “Hongyang” and “Cuiyu” kiwifruit at harvest under grown under open-field (OF) and rain-shelter (RS) cultivation systems. Disease incidence in kiwifruit was significantly lower (p < 0.05) under the RS system than it was under the OF system. High-throughput sequencing [16S V3-V4 ribosomal region and the fungal internal transcribed spacer (ITS2)] was conducted to compare the composition of the epiphytic and endophytic microbial community of kiwifruit under the two cultivation systems. Results indicated that the abundance of Actinobacteria, Bacteroidetes, Enterobacteriales, Acetobacterales, Sphingomonas, Pseudomonas, and Sphingobacterium was higher under the RS system, relative to the OF system, while the abundance of Capnodiales, Hypocreales, Vishniacozyma, and Plectosphaerella was also higher under the RS system. Some of these bacterial and fungal taxa have been reported to as act as biocontrol agents and reduce disease incidence. Notably, the α-diversity of the epiphytic bacterial and fungal communities on kiwifruit was higher under RS cultivation. In summary, RS cultivation reduced natural disease incidence in kiwifruit, which may be partially attributed to differences in the structure and composition of the microbial community present in and on kiwifruit.
tRNA Metabolism and Lung Cancer: Beyond Translation
Authors: Bian Meng , Huang Shiqiong , Yu Dongsheng , Zhou Zheng
Published: September 2021

Lung cancer, one of the most malignant tumors, has extremely high morbidity and mortality, posing a serious threat to global health. It is an urgent need to fully understand the pathogenesis of lung cancer and provide new ideas for its treatment. Interestingly, accumulating evidence has identified that transfer RNAs (tRNAs) and tRNA metabolism–associated enzymes not only participate in the protein translation but also play an important role in the occurrence and development of lung cancer. In this review, we summarize the different aspects of tRNA metabolism in lung cancer, such as tRNA transcription and mutation, tRNA molecules and derivatives, tRNA-modifying enzymes, and aminoacyl-tRNA synthetases (ARSs), aiming at a better understanding of the pathogenesis of lung cancer and providing new therapeutic strategies for it.
The Calcium Channel α2δ1 Subunit: Interactional Targets in Primary Sensory Neurons and Role in Neuropathic Pain
Authors: Cui Wenqiang , Wu Hongyun , Yu Xiaowen , Song Ting , Xu Xiangqing , Xu Fei
Published: September 2021

Neuropathic pain is mainly triggered after nerve injury and associated with plasticity of the nociceptive pathway in primary sensory neurons. Currently, the treatment remains a challenge. In order to identify specific therapeutic targets, it is necessary to clarify the underlying mechanisms of neuropathic pain. It is well established that primary sensory neuron sensitization (peripheral sensitization) is one of the main components of neuropathic pain. Calcium channels act as key mediators in peripheral sensitization. As the target of gabapentin, the calcium channel subunit α2δ1 (Cavα2δ1) is a potential entry point in neuropathic pain research. Numerous studies have demonstrated that the upstream and downstream targets of Cavα2δ1 of the peripheral primary neurons, including thrombospondins, N-methyl-D-aspartate receptors, transient receptor potential ankyrin 1 (TRPA1), transient receptor potential vanilloid family 1 (TRPV1), and protein kinase C (PKC), are involved in neuropathic pain. Thus, we reviewed and discussed the role of Cavα2δ1 and the associated signaling axis in neuropathic pain conditions.
Case Report: Laryngospasm as Initial Manifestation of Amyotrophic Lateral Sclerosis in a Long-Survival Patient With Heterozygous p.D90A – SOD1 Mutation
Authors: Capece Giuliana , Ceroni Mauro , Alfonsi Enrico , Palmieri Ilaria , Cereda Cristina , Diamanti Luca
Published: September 2021

Amyotrophic Lateral Sclerosis (ALS) is a fatal neurodegenerative disease affecting motor neurons. Although its etiology is still unknown, many genes have been found to be implicated in ALS pathogenesis. The Cu/Zn superoxide dismutase (SOD1) gene was the first to be identified. Currently, more than 230 mutations in the SOD1 gene have been reported. p.D90A (p. Asp90Ala) is the most common SOD1 mutation worldwide. It shows both autosomal and recessive inheritance in different populations. To date, five Italian patients with the heterozygous p.D90A mutation have been reported. None of them complained of laryngological symptoms as the initial manifestation of ALS, although they had atypical clinical features. We describe a long-survival patient carrying heterozygous p.D90A mutation who presented with severe laryngospasm due to bilateral vocal cord paralysis. We suggest that genetic analysis may help to diagnose ALS with insidious onset like hoarseness, laryngospasm, and other type of voice disturbances.
Neuropsychiatric Symptoms in Behavioral Variant Frontotemporal Dementia and Alzheimer's Disease: A 12-Month Follow-Up Study
Authors: Da Silva Thais Bento Lima , Ordonez Tiago Nascimento , Bregola Allan Gustavo , Bahia Valéria Santoro , Cecchini Mário Amore , Guimarães Henrique Cerqueira , Gambogi Leandro Boson , Caramelli Paulo , Balthazar Marcio Luiz Figueredo , Damasceno Benito Pereira , Brucki Sonia Maria Dozzi , de Souza Leonardo Cruz , Nitrini Ricardo , Yassuda Monica Sanches
Published: September 2021

Introduction: Neuropsychiatric symptoms in patients with frontotemporal dementia (FTD) are highly prevalent and may complicate clinical managements.

Objective: To test whether the Neuropsychiatry Inventory (NPI) could detect change in neuropsychiatric symptoms and caregiver's distress in patients diagnosed with behavioral variant frontotemporal dementia (bvFTD) and Alzheimer's disease (AD) from baseline to a 12-month follow-up and to investigate possible predictors of change in NPI scores.

Methods: The sample consisted of 31 patients diagnosed with bvFTD and 28 patients with AD and their caregivers. The Mini-Mental State Examination (MMSE), Addenbrooke's Cognitive Examination Revised (ACE-R), the INECO Frontal Screening (IFS), the Frontal Assessment Battery (FAB), the Executive Interview (EXIT-25) and the NPI were applied. Descriptive statistics, Mann-Whitney U test, Wilcoxon test, Chi square (χ2) test and Linear Regression Analysis were used.

Results: NPI total and caregiver distress scores were statistically higher among bvFTD patients at both assessment points. MMSE, ACE-R scores significantly declined and NPI Total and Distress scores significantly increased in both groups. In the bvFTD group, age was the only independent predictor variable for the NPI total score at follow up. In the AD group, ACE-R and EXIT-25, conjunctively, were associated with the NPI total score at follow up.

Conclusions: In 12 months, cognition declined and neuropsychiatric symptoms increased in bvFTD and AD groups. In the AD group only, cognitive impairment was a significant predictor of change in neuropsychiatric symptoms.
Recurrence Rates After Microvascular Decompression in Patients With Primary Trigeminal Neuralgia and Its Influencing Factors: A Systematic Review and Meta-Analysis Based on 8,172 Surgery Patients
Authors: Chen Fangyu , Niu Yuming , Meng Fan , Xu Pan , Zhang Chao , Xue Yingying , Wu Shishi , Wang Long
Published: September 2021

Background: Primary trigeminal neuralgia (PTN) is known to reoccur following microvascular decompression (MVD) surgery. However, the rates and contributing factors related to PTN recurrence remain controversial. The purpose of this study was to explore the postoperative recurrence rates and related influencing factors of patients with PTN after MVD. Additionally, recurrence rates after different treatments were compared to provide guidelines for clinicians.

Methods: We conducted systematic reviews and meta-analyses in accordance with the preferred reporting items of the PRISMA guidelines. We searched nine databases, namely, the PubMed, EMBASE, Cochrane Library, Web of Science, CINAHL, CBM, CNKI, VIP, and Wanfang databases, from establishment to July 13, 2020, selecting for studies about the long-term postoperative efficacy of MVD in the treatment of PTN. Factors associated with higher recurrence rates after MVD and long-term postoperative results of other treatments underwent formal meta-analysis, where odds ratios (ORs) with the corresponding 95% confidence intervals (CIs) were calculated. The dose-response model was used to inspect the associations between several factors and higher recurrence rates.

Results: Seventy-four studies (8,172 patients, 32 case series studies, and 42 non-randomized controlled trials) were analyzed in our research. Overall, 956 out of 8,172 patients relapsed, and the pooled recurrence rate was 0.096 (0.080–0.113). Influencing factors of relatively higher recurrence rates included atypical trigeminal neuralgia symptoms, lack of nerve groove, non-arterial compression, patients who were 50–60 years old, and longer disease duration. Dose–response analysis showed that the recurrence rate had a significant trend with the published year and the follow-up time. Simultaneously, the recurrence rate of MVD treatment was much lower than that of conventional drug treatment, gamma knife surgery, percutaneous balloon compression, and radiofrequency thermocoagulation. When the surgical technique was improved or combined with partial sensory rhizotomy (PSR), the postoperative recurrence rates were significantly reduced.

Conclusions: Even for PTN patients who have a successful operation, ~10% of them will still relapse. This research identifies several factors that can affect the recurrence rate. Compared with other operations, MVD has a relatively lower recurrence rate. Our analysis suggests that improved surgical techniques and combining PSR and MVD will yield better results.

Systematic Review Registration:https://www.crd.york.ac.uk/PROSPERO/, identifier: CRD42020159276.
Intracranial Stenting as Rescue Therapy After Failure of Mechanical Thrombectomy for Basilar Artery Occlusion: Data From the ANGEL-ACT Registry
Authors: Luo Gang , Gao Feng , Zhang Xuelei , Jia Baixue , Huo Xiaochuan , Liu Raynald , Chi Man Sum , Ma Gaoting , Peng Guangge , Zhang Jingyu , Qi Zhongqi , Guo Xu , Han Bin , Tong Xu , Wang Bo , Song Ligang , Liu Lian , He Zijun , Mo Dapeng , Ma Ning , Sun Xuan , Yang Ming , Miao Zhongrong
Published: September 2021

Background and Purpose: Studies on rescue therapy for acute posterior circulation stroke due to basilar artery occlusion (BAO) are limited in the modern era of mechanical thrombectomy (MT). The aim of this study was to evaluate the safety and efficacy of rescue stenting (RS) following MT failure in patients with BAO.

Methods: Data were collected from the Endovascular Treatment Key Technique and Emergency Work Flow Improvement of Acute Ischemic Stroke (ANGEL-ACT) prospective registry in China. Patients who underwent MT for BAO with failure of recanalization were enrolled in this study. The patients were divided into the RS and non-RS groups. Clinical and laboratory findings, procedural details, and clinical outcomes were compared between the two groups.

Results: Overall, 93 patients with acute BAO were analyzed. The RS group included 81 (87.1%) patients, and the non-RS group included 12 patients. A modified treatment in cerebral infarction (mTICI) score of 2b/3 was achieved in 75 (92.6%) patients in the RS group. Compared with the non-RS group, the RS group had a significantly higher rate of successful recanalization and favorable clinical outcomes (modified Rankin Scale score at 90 days post-procedure, 0–3: 16.7 vs. 51.9%, respectively; P = 0.023) without an increase in the rate of symptomatic intracranial hemorrhage and a significantly lower mortality rate (58.3 vs. 18.5%, respectively; P = 0.006). Furthermore, the use of a glycoprotein IIb/IIIa inhibitor improved the rate of recanalization of the target artery without increasing the rate of symptomatic intracranial hemorrhage.

Conclusions: Permanent stenting appears to be a feasible rescue modality when MT fails and might provide functional benefits in patients with acute ischemic stroke due to BAO.
Machine Learning for Subtyping Concussion Using a Clustering Approach
Authors: Rosenblatt Cirelle K. , Harriss Alexandra , Babul Aliya-Nur , Rosenblatt Samuel A.
Published: September 2021

Background: Concussion subtypes are typically organized into commonly affected symptom areas or a combination of affected systems, an approach that may be flawed by bias in conceptualization or the inherent limitations of interdisciplinary expertise.

Objective: The purpose of this study was to determine whether a bottom-up, unsupervised, machine learning approach, could more accurately support concussion subtyping.

Methods: Initial patient intake data as well as objective outcome measures including, the Patient-Reported Outcomes Measurement Information System (PROMIS), Dizziness Handicap Inventory (DHI), Pain Catastrophizing Scale (PCS), and Immediate Post-Concussion Assessment and Cognitive Testing Tool (ImPACT) were retrospectively extracted from the Advance Concussion Clinic's database. A correlation matrix and principal component analysis (PCA) were used to reduce the dimensionality of the dataset. Sklearn's agglomerative clustering algorithm was then applied, and the optimal number of clusters within the patient database were generated. Between-group comparisons among the formed clusters were performed using a Mann-Whitney U test.

Results: Two hundred seventy-five patients within the clinics database were analyzed. Five distinct clusters emerged from the data when maximizing the Silhouette score (0.36) and minimizing the Davies-Bouldin score (0.83). Concussion subtypes derived demonstrated clinically distinct profiles, with statistically significant differences (p < 0.05) between all five clusters.

Conclusion: This machine learning approach enabled the identification and characterization of five distinct concussion subtypes, which were best understood according to levels of complexity, ranging from Extremely Complex to Minimally Complex. Understanding concussion in terms of Complexity with the utilization of artificial intelligence, could provide a more accurate concussion classification or subtype approach; one that better reflects the true heterogeneity and complex system disruptions associated with mild traumatic brain injury.
Changes of Plasma Amino Acid Profiles in Infants With a Nutrient-Fortified Complementary Food Supplement: Evidence From a 12-Month Single-Blind Cluster-Randomized Controlled Trial
Authors: Furuta Chie , Sato Wataru , Murakami Hitoshi , Suri Devika J. , Otoo Gloria E. , Tano-Debrah Kwaku , Ghosh Shibani A.
Published: September 2021

Stunting is reportedly associated with low circulating levels of essential amino acids (EAAs). This study examined the effect of a macronutrient- and micronutrient-fortified complementary food supplement (KOKO Plus) on specific plasma EAA levels and stunting in infants aged 6–18 months. In a single-blind cluster-randomized controlled trial conducted in Ghana, infants were enrolled at 6 months and followed until 18 months. Thirty-eight communities were randomly assigned to receive KOKO Plus (KP, fourteen communities, n = 321), multiple-micronutrient powder (MN, thirteen communities, n = 327), or only nutritional education as control group (NE, eleven communities, n = 318), and all groups received nutrition education. Plasma amino acids (AAs) were measured at 6, 12, and 18 months (end point). Mixed-effects models were used to assess the effect of the intervention on plasma AAs, and the relationship between plasma branched-chain AAs (BCAAs) and the risk of stunting was assessed. At the end point, total BCAA concentrations (±standard error) significantly exceeded baseline in the KP (284.2 ± 4.3 μM) and NE (289.1 ± 4.4 μM) groups but not the MN group (264.4 ± 4.1 μM). After adjustment for compliance at 200 sachets, plasma BCAAs exceeded in the KP group (284.5 ± 4.2 μM) compared to the MN group (264.6 ± 4 μM). Plasma BCAAs were positively correlated with changes in length-for-age Z-score from baseline (R = 0.327, p = 0.048). In conclusion, the plasma BCAA concentrations of infants that received KP and the NE group was significantly higher compared to the MN group but there were no differences between the KP and NE group at end point. Improved plasma BCAAs may be due to improved nutrient intake by infants exposed to KP or NE. Low BCAAs were associated with stunting, replicating the previous finding.

Clinical Trial Registration:https://clinicaltrials.gov/ct2/show/NCT03181178?term=NCT03181178&draw=2&rank=1, identifier: NCT03181178.
Improving of Gelling Capacity of Cooked Crab Meat Proteins
Authors: Trejo-Díaz Gabriela Nallely , Martínez-Maldonado Miguel Ángel , Uresti-Marín Rocío M. , Velazquez Gonzalo , Ramírez José Alberto
Published: September 2021

Cooked crab meat subjected to a cutting process can aggregate again, forming weak gels. The objective of this work was to determine the effect of two mixing methods, combined with the addition of the microbial enzyme TGase (MTGase) on the mechanical and functional properties of gels from washed or unwashed blue crab (Callinectes sapidus) meat. Live crabs were obtained from Laguna Madre, Tamaulipas, Mexico, and cooked at 120°C for 20 min before hand-picking the meat from the shell. Cooked meat was processed by mixing and cut at temperatures of 25 or 60°C, without (control) or 0.5% of MTGase. Then cooked at 90°C for 15 min. Changes in texture profile analysis, percentage of extractable water, and color were evaluated. The mixing method at 60°C allowed increasing the textural properties of the gels, and the addition of MTGase significantly improved the mechanical properties. The results allowed stablishing a viable technique to obtain restructured gels from cooked crab meat with no need to extract the soluble compounds responsible for their distinctive odor and taste which often affect the mechanical properties.
Advanced Lung Cancer Inflammation Index Predicts Survival Outcomes of Patients With Oral Cavity Cancer Following Curative Surgery
Authors: Tsai Yao-Te , Hsu Cheng-Ming , Chang Geng-He , Tsai Ming-Shao , Lee Yi-Chan , Huang Ethan I. , Lai Chia-Hsuan , Fang Ku-Hao
Published: September 2021

Aim

The aim of our study was to investigate the prognostic value of preoperative advanced lung cancer inflammation index (ALI) and to establish prognostic nomograms for the prediction of survival outcomes in patients with oral cavity squamous cell carcinoma (OSCC).

Materials and Methods

A total of 372 patients who received primary curative surgery for OSCC during 2008–2017 at a tertiary referral center were enrolled. We used the receiver operating characteristic curve to determine the optimal cutoff point of ALI. Through a Cox proportional hazards model and Kaplan–Meier analysis, we elucidated the ALI–overall survival (OS) and ALI–disease-free survival (DFS) associations. Prognostic nomograms based on ALI and the results of multivariate analysis were created to predict the OS and DFS. We used the concordance indices (C-indices) and calibration plots to assess the discriminatory and predictive ability.

Results

The results revealed that the ALI cutoff was 33.6, and 105 and 267 patients had ALI values of <33.6 and ≥33.6, respectively. ALI < 33.6 significantly indicated lower OS (44.0% vs. 80.1%, p < 0.001) and DFS (33.6% vs. 62.8%; p < 0.001). In multivariate analysis, ALI < 33.6 was independently associated with poor OS and DFS (both p < 0.001). The C-indices of established nomograms were 0.773 and 0.674 for OS and DFS, respectively; moreover, the calibration plots revealed good consistency between nomogram-predicted and actual observed OS and DFS.

Conclusion

ALI is a promising prognostic biomarker in patients undergoing primary surgery for OSCC; moreover, ALI-based nomograms may be a useful prognostic tool for individualized OS and DFS estimations.