bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of Long-Term Oncological Results in Young Women with Breast Cancer between BRCA-Mutation Carriers Versus Non-Carriers: How Tumor and Genetic Risk Factors Influence the Clinical Prognosis
Authors: Tinterri Corrado , Di Maria Grimaldi Simone , Sagona Andrea , Barbieri Erika , Darwish Shadya , Bottini Alberto , Canavese Giuseppe , Gentile Damiano
Published: August 2023

Background: Breast cancer (BC) is very uncommon in young women (YW) and it is unclear whether a BRCA mutation has prognostic implications. Our aim was to evaluate the characteristics of YW with BC by comparing the long-term oncological results between BRCA-mutation carriers and non-carriers. Methods: We retrospectively reviewed all the consecutive YW (aged 18–40 years) diagnosed with BC. Endpoints were disease-free survival (DFS), distant disease-free survival (DDFS), and overall survival (OS). Results: 63 YW with a BRCA mutation were compared with 339 YW without BRCA mutation. BRCA-mutation carriers were younger (60.3% versus 34.8% if age ≤ 35 years, p = 0.001) and presented with more aggressive tumors (66.7% versus 40.7% if G3, p = 0.001; 57.2% versus 12.4% if biological subtype triple-negative, p = 0.001; 73.0% versus 39.2% if Ki67 ≥ 25%, p = 0.001). Non-carriers presented significantly better DFS, DDFS, and OS compared with BRCA-mutation carriers. Neoadjuvant chemotherapy was found to be an independent protective factor for OS in BRCA-mutation carriers. Conclusions: BC is more likely to present at a younger age (≤ 35 years) and with more aggressive characteristics (G3, triple-negative, Ki67 ≥ 25%) in YW with BRCA mutation compared with their non-mutated counterparts. Young BRCA-mutation carriers showed a poorer prognosis in terms of recurrence and survival compared with non-carriers. The implementation of neoadjuvant chemotherapy may improve survival in YW with BC and BRCA mutation.
Integrative Evaluation of the Clinical Significance Underlying Protein Arginine Methyltransferases in Hepatocellular Carcinoma
Authors: Jiang Yikun , Wei Shibo , Koo Jin-Mo , Kim Hea-Ju , Park Wonyoung , Zhang Yan , Guo He , Ha Ki-Tae , Oh Chang-Myung , Kang Jong-Sun , Jeong Jee-Heon , Ryu Dongryeol , Kim Kyeong-Jin , Jo Yunju
Published: August 2023

HCC is a major contributor to cancer-related mortality worldwide. Curative treatments are available for a minority of patients diagnosed at early stages; however, only a few multikinase inhibitors are available and are marginally effective in advanced cases, highlighting the need for novel therapeutic targets. One potential target is the protein arginine methyltransferase, which catalyzes various forms of arginine methylation and is often overexpressed in various cancers. However, the diverse expression patterns and clinical values of PRMTs in HCC remain unclear. In the present study, we evaluated the transcriptional expression of PRMTs in HCC cohorts using publicly available datasets. Our results revealed a significant association between PRMTs and prognosis in HCC patients with diverse clinical characteristics and backgrounds. This highlights the promising potential of PRMTs as prognostic biomarkers in patients with HCC. In particular, single-cell RNA (scRNA) sequencing analysis coupled with another human cohort study highlighted the pivotal role of PRMT1 in HCC progression, particularly in the context of Tex. Translating these findings into specific therapeutic decisions may address the unmet therapeutic needs of patients with HCC.
Efficacy of Empirical Radioiodine Therapy in Patients with Differentiated Thyroid Cancer and Elevated Serum Thyroglobulin without Evidence of Structural Disease: A Propensity Score Analysis
Authors: Piscopo Leandra , Zampella Emilia , Volpe Fabio , Gaudieri Valeria , Nappi Carmela , Cutillo Paolo , Volpicelli Federica , Falzarano Maria , Pace Leonardo , Cuocolo Alberto , Klain Michele
Published: August 2023

We assessed the outcome of administration of empiric radioactive iodine (RAI) therapy to patients with differentiated thyroid cancer (DTC), in a propensity-score-matched cohort of patients with biochemical incomplete response (BIR) and without evidence of structural disease. We retrospectively evaluated 820 DTC patients without distant metastases, who underwent total thyroidectomy followed by RAI therapy, with available BIR at 12 months and follow-up evaluations. The patients were categorized according to the administration of empiric therapy (ET). To account for differences between patients with (n = 119) and without (n = 701) ET, a propensity-score-matched cohort of 119 ET and 119 no-ET patients was created. The need for additional therapy and the occurrence of structural disease were considered as end-points. During a median follow-up of 53 months (range 3–285), 57 events occurred (24% cumulative event rate). The rate of events was significantly higher in the no-ET compared to the ET patients (30% vs. 18% p < 0.001). The multivariate Cox analysis identified age (p < 0.01), pre-therapy Tg (p < 0.05) and empiric RAI therapy (p < 0.01) as predictors of outcome. The Kaplan–Meier analysis found that progression-free survival was lower in no-ET patients compared to the ET group (p < 0.01). In patients with DTC treated with surgery and RAI, and with biochemical incomplete response at the 12-month evaluation, their prognosis seemed to be affected by Tg values and the empiric treatment. The identification of candidates for this approach may improve prognosis.
Separation of Floodplain Flow and Bankfull Discharge: Application of 1D Momentum Equation Solver and MIKE 21C
Authors: Sarker Shiblu
Published: August 2023

A floodplain is an area of low-lying land adjacent to a river, stream, or other water body that is regularly inundated by water during periods of high flow. Floodplains typically have relatively flat terrain and are composed of sediments deposited by the river over time. Floodplain flow refers to the movement of water across the surface of the floodplain during periods of high flow. This flow can occur as a result of water spilling over the river banks or seeping into the ground and then re-emerging on the surface of the floodplain. Bankfull discharge is the flow of water that just fills the channel of a river or stream to the top of its banks. It is the point at which the river or stream is at its maximum capacity without overflowing onto the floodplain. Bankfull discharge is often used as a reference point for assessing flood risk and planning floodplain management strategies. To examine the bank-to-bank hydro-morphodynamics of a river, it is necessary to comprehend the flow distribution throughout the main stream and floodplain. Along with river hydraulics, bankfull discharge is a crucial parameter for estimating river bank erosion. For evaluating the distribution and generation of river flow over the floodplain and main stream, a variety of modeling tools and approaches are available. This study investigates methods for separating floodplain flow and bankfull discharge from observed discharge data using the one-dimensional momentum equation. A two-dimensional modeling tool (MIKE 21C) was also employed to investigate the usefulness of the proposed method in a region with an enormous floodplain.
Regulation of Phosphate Transporters and Novel Regulator of Phosphate Metabolism
Authors: Koike Megumi , Uga Minori , Shiozaki Yuji , Miyamoto Ken-ichi , Segawa Hiroko
Published: August 2023

Phosphorus is essential for all living organisms. It plays an important role in maintaining biological functions, such as energy metabolism, cell membrane formation, and bone mineralization. Various factors in the intestine, kidneys, and bones regulate the homeostasis of the inorganic phosphate (Pi) concentration in the body. X-linked hypophosphatemia (XLH), the most common form of hereditary hypophosphatemic rickets, is characterized by an impaired mineralization of the bone matrix, hypertrophic chondrocytes with hypophosphatemia, and active vitamin D resistance in childhood. Phosphate-regulating gene with homologies to endopeptidases on the X chromosome was recognized as the responsible gene for XLH. XLH is classified as fibroblast growth factor 23 (FGF23)-related hypophosphatemic rickets. The enhanced FGF23 stimulates renal phosphate wasting by downregulating sodium-dependent Pi cotransporters, NaPi2a and NaPi2c proteins, in the proximal tubules. Recently, transmembrane protein (Tmem) 174 has been identified as a novel regulator of phosphate transporters. This review introduces the role of Tmem174 in the Pi homeostasis in the body.
Multiomics Data Analysis Identified CpG Sites That Mediate the Impact of Smoking on Cardiometabolic Traits
Authors: Nikpay Majid
Published: August 2023

Understanding the epigenome paths through which smoking contributes to cardiometabolic traits is important for downstream applications. In this study, an SNP-based analytical pipeline was used to integrate several publicly available datasets in order to identify CpG sites that mediate the impact of smoking on cardiometabolic traits and to investigate the underlying molecular mechanisms. After applying stringent statistical criteria, 11 CpG sites were detected that showed significant association (p < 5 × 10−8) with cardiometabolic traits at both the discovery and replication stages. By integrating eQTL data, I found genes behind a number of these associations. cg05228408 was hypomethylated in smokers and contributed to higher blood pressure by lowering the expression of the CLCN6 gene. cg08639339 was hypermethylated in smokers and lowered the metabolic rate by increasing the expression of RAB29; furthermore, I noted TMEM120A mediated the impact of smoking-cg17325771 on LDL, and LTBP3 mediated the smoking-cg07029024 effect on heart rate. The pathway analysis identified processes through which the identified genes impact their traits. This study provides a list of CpG sites that mediates the impact of smoking on cardiometabolic traits and a framework to investigate the underlying molecular paths using publicly available data.
Ecological Stoichiometry of N and P across a Chronosequence of Chinese Fir Plantation Forests
Authors: Cao Juan , Yan Wende , Farooq Taimoor Hassan , Chen Xiaoyong , Wang Jun , Yuan Chenglin , Qi Yaqin , Khan Khalid Ali
Published: August 2023

Ecological stoichiometry is crucial in understanding nutrient dynamics and its impact on plant growth and development at various ecological scales. Among the different nutrients, nitrogen (N) and phosphorus (P) have been widely recognized as key elements regulating substance transport, energy utilization, and ecosystem conversion. The N:P ratio in plants serves as a sensitive indicator of ecological processes, reflecting the availability and balance of these nutrients. Therefore, studying the ecological stoichiometry of N and P is essential for accurately assessing soil fertility and site productivity, particularly in forest ecosystems with low-fertility soils. In this study conducted in Huitong, Hunan province, southern China, the contents of N and P, as well as the N:P ratios, were investigated in plant-soil systems across four different aged stands of Chinese fir forests (3-, 8-, 18-, and 26-year-old stands). The results revealed varying concentrations of N and P in soils and foliage across the different plantations. Soil N concentrations increased by approximately 4%, 30%, and 22% in 8-, 18-, and 26-year-old plantations compared to the 3-year-old plantation. Soil P concentration was significantly higher in 8-, 18-, and 26-year-old plantations compared to the 3-year-old plantation. The average soil N:P ratio followed the order of 3-year-old plantation > 18-year-old plantation > 26-year-old plantation > 8-year-old plantation. Regarding foliage, both N and P contents exhibited a similar pattern across the different aged leaves, with current-year-old leaves having higher concentrations than 1-year-old, 2-year-old, and 3-year-old leaves in all four Chinese fir plantations. The study further established relationships between soil and foliage nutrient ratios. Soil N:P ratio was positively correlated with soil N content but negatively associated with soil P content. The foliage N:P ratio also showed a significant negative correlation between leaf N and foliage P content. These findings suggest that soil nutrient conditions improved with the aging of Chinese fir plantations, mainly due to increased inputs of above- and below-ground litter. Overall, this study provides valuable insights into the ecological stoichiometry of N and P in Chinese fir plantations, offering a scientific basis for sustainable forest management practices in southern China.
Comparing Forest Understory Fuel Classification in Portugal Using Discrete Airborne Laser Scanning Data and Satellite Multi-Source Remote Sensing Data
Authors: Mihajlovski Bojan , Fernandes Paulo M. , Pereira José M. C. , Guerra-Hernández Juan
Published: August 2023

Wildfires burn millions of hectares of forest worldwide every year, and this trend is expected to continue growing under current and future climate scenarios. As a result, accurate knowledge of fuel conditions and fuel type mapping are important for assessing fire hazards and predicting fire behavior. In this study, 499 plots in six different areas in Portugal were surveyed by ALS and multisource RS, and the data thus obtained were used to evaluate a nationwide fuel classification. Random Forest (RF) and CART models were used to evaluate fuel models based on ALS (5 and 10 pulse/m2), Sentinel Imagery (Multispectral Sentinel 2 (S2) and SAR (Synthetic Aperture RaDaR) data (C-band (Sentinel 1 (S1)) and Phased Array L-band data (PALSAR-2/ALOS-2 Satellite) metrics. The specific goals of the study were as follows: (1) to develop simple CART and RF models to classify the four main fuel types in Portugal in terms of horizontal and vertical structure based on field-acquired ALS data; (2) to analyze the effect of canopy cover on fuel type classification; (3) to investigate the use of different ALS pulse densities to classify the fuel types; (4) to map a more complex classification of fuel using a multi-sensor approach and the RF method. The results indicate that use of ALS metrics (only) was a powerful way of accurately classifying the main four fuel types, with OA = 0.68. In terms of canopy cover, the best results were estimated in sparse forest, with an OA = 0.84. The effect of ALS pulse density on fuel classification indicates that 10 points m−2 data yielded better results than 5 points m−2 data, with OA = 0.78 and 0.71, respectively. Finally, the multi-sensor approach with RF successfully classified 13 fuel models in Portugal, with moderate OA = 0.44. Fuel mapping studies could be improved by generating more homogenous fuel models (in terms of structure and composition), increasing the number of sample plots and also by increasing the representativeness of each fuel model.
Novel α-1,3-Glucosyltransferase Variants and Their Broad Clinical Polycystic Liver Disease Spectrum
Authors: Boerrigter Melissa M. , te Morsche René H. M. , Venselaar Hanka , Pastoors Nikki , Geerts Anja M. , Hoorens Anne , Drenth Joost P. H.
Published: August 2023

Protein-truncating variants in α-1,3-glucosyltransferase (ALG8) are a risk factor for a mild cystic kidney disease phenotype. The association between these variants and liver cysts is limited. We aim to identify pathogenic ALG8 variants in our cohort of autosomal dominant polycystic liver disease (ADPLD) individuals. In order to fine-map the phenotypical spectrum of pathogenic ALG8 variant carriers, we performed targeted ALG8 screening in 478 ADPLD singletons, and exome sequencing in 48 singletons and 4 patients from two large ADPLD families. Eight novel and one previously reported pathogenic variant in ALG8 were discovered in sixteen patients. The ALG8 clinical phenotype ranges from mild to severe polycystic liver disease, and from innumerable small to multiple large hepatic cysts. The presence of <5 renal cysts that do not affect renal function is common in this population. Three-dimensional homology modeling demonstrated that six variants cause a truncated ALG8 protein with abnormal functioning, and one variant is predicted to destabilize ALG8. For the seventh variant, immunostaining of the liver tissue showed a complete loss of ALG8 in the cystic cells. ALG8-associated ADPLD has a broad clinical spectrum, including the possibility of developing a small number of renal cysts. This broadens the ADPLD genotype–phenotype spectrum and narrows the gap between liver-specific ADPLD and kidney-specific ADPKD.
Changes in m6A in Steatotic Liver Disease
Authors: Petri Belinda J. , Cave Matthew C. , Klinge Carolyn M.
Published: August 2023

Fatty liver disease is one of the major causes of morbidity and mortality worldwide. Fatty liver includes non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH), now replaced by a consensus group as metabolic dysfunction-associated steatotic liver disease (MASLD). While excess nutrition and obesity are major contributors to fatty liver, the underlying mechanisms remain largely unknown and therapeutic interventions are limited. Reversible chemical modifications in RNA are newly recognized critical regulators controlling post-transcriptional gene expression. Among these modifications, N6-methyladenosine (m6A) is the most abundant and regulates transcript abundance in fatty liver disease. Modulation of m6A by readers, writers, and erasers (RWE) impacts mRNA processing, translation, nuclear export, localization, and degradation. While many studies focus on m6A RWE expression in human liver pathologies, limitations of technology and bioinformatic methods to detect m6A present challenges in understanding the epitranscriptomic mechanisms driving fatty liver disease progression. In this review, we summarize the RWE of m6A and current methods of detecting m6A in specific genes associated with fatty liver disease.
The Danish Trauma Database for Refugees (DTD): A Multicenter Database Collaboration—Overcoming the Challenges and Enhancing Mental Health Treatment and Research for Refugees
Authors: Thøgersen Marie Høgh , Bager Line , Bangsgaard Sofie Grimshave , Palic Sabina , Auning-Hansen Mikkel , Møller Stine Bjerrum , Larsen Kirstine Bruun , Tækker Louise , Jensen Bo Søndergaard , Bothe Søren , Nordin Linda
Published: August 2023

Mental health of trauma-affected refugees is an understudied area, resulting in inadequate and poorer treatment outcomes. To address this, more high-quality treatment studies that include predictive analyses, long-term evaluations, cultural adaptations, and take account for comorbidities, are needed. Moreover, given the complex intertwining of refugees’ health with post-migration stressors and other social factors, it is crucial to examine the social determinants of refugee mental health. The Danish Trauma Database for Refugees (DTD) is a multicenter research database uniting six national centers that provide outpatient treatment for trauma-affected refugees. Through the database, we collect clinical and sociodemographic data from approximately 1200 refugees annually and will merge the database with Danish population register data. The purpose of the DTD is two-fold; clinical and research. The DTD offers data-driven guidance for routine clinical treatment planning of the individual patient, as well as exceptional research opportunities for testing treatment interventions in clinical settings, with larger sample sizes, and more representative heterogeneity of the population. Complex analyses of risk and protective factors, barriers, access to treatment, and societal and transgenerational aspects of trauma are possible with the DTD. This conceptual paper introduces the DTD, the historical background, the development process and implementation strategy, and the associated challenges with developing and running a multicenter database. Most importantly, it highlights the clinical and research potential of the DTD for advancing the understanding and treatment of trauma-affected refugees.
Influence of Sociodemographic, Organizational, and Social Factors on Turnover Consideration Among Eldercare Workers: A Quantitative Survey
Authors: Krsnik Sabina , Erjavec Karmen
Published: August 2023

Staff turnover in long-term care (LTC) is considered one of the main causes of staff shortages and a key problem for LTC systems in the developed world. Factors affecting staff turnover in LTC facilities are poorly understood due to a fragmented approach. The aim of this study was to use multivariate analysis to identify the factors at the macro-, meso-, and micro-level that influence LTC workers’ turnover in Slovenia, a typical Central and Eastern European country. A correlational cross-sectional survey design with a self-reported online questionnaire was used among Slovenian LTC workers (N = 452). The results show that more than half of LTC workers intend to quit their jobs and leave the LTC sector. LTC workers who intend to leave are generally younger, have worked in the LTC sector for a shorter period, are mainly employed in the public sector, especially in nursing homes, and earn less. The connection between the intention to leave and the factors at the macro-, meso-, and micro-level is very high. Over 75% of the variance of intention to leave was explained by the linear influence of sociodemographic characteristics, social recognition, and work environment. Urgent measures for improving the work environment are needed.
Healthy Food Policies Documented in University Food Service Contracts
Authors: Fandetti Stacy M. , Dahl Alicia Anne , Webster Caitlan , Bably Morium Barakat , Coffman Maren J. , Racine Elizabeth F.
Published: August 2023

In the United States, there is an opportunity to improve the nutritional health of university students through the campus food environment. This project used a content analysis approach to investigate whether healthy food standards and policies were incorporated into the contract agreements between North Carolina (NC) public universities and their food service management companies. Food service contracts were collected from 14 NC public universities using food service management companies on campus. Each contract was evaluated using the 35-item North Carolina Food Service Policy Guidelines Assessment to examine four elements of the campus food environment: Beverages, Packaged Snacks, Prepared Foods, and Other (e.g., strategic placement of healthier food). Five university food service contracts incorporated no North Carolina Food Service Policy Guidelines, three university contracts included one to five guidelines, and six university contracts included six to nine guidelines. Altogether, 13 of the 35 guidelines were incorporated into at least one university food service contract. This project presents a cost and time-effective assessment method for determining if evidence-based nutrition guidelines have been included in university food service contracts. This approach and findings may lead to contract revisions to improve the campus food environment and, subsequently, the nutritional health of college populations.
Potential Role of Antibodies against Aquaporin-1 in Patients with Central Nervous System Demyelination
Authors: Pechlivanidou Maria , Xenou Konstantina , Tzanetakos Dimitrios , Koutsos Emmanuel , Stergiou Christos , Andreadou Elisabeth , Voumvourakis Konstantinos , Giannopoulos Sotirios , Kilidireas Constantinos , Tüzün Erdem , Tsivgoulis Georgios , Tzartos Socrates , Tzartos John
Published: August 2023

Aquaporins (AQPs; AQP0–AQP12) are water channels expressed in many and diverse cell types, participating in various functions of cells, tissues, and systems, including the central nervous system (CNS). AQP dysfunction and autoimmunity to AQPs are implicated in several diseases. The best-known example of autoimmunity against AQPs concerns the antibodies to AQP4 which are involved in the pathogenesis of neuromyelitis optica spectrum disorder (NMOSD), an autoimmune astrocytopathy, causing also CNS demyelination. The present review focuses on the discovery and the potential role of antibodies against AQP1 in the CNS, and their potential involvement in the pathophysiology of NMOSD. We describe (a) the several techniques developed for the detection of the AQP1-antibodies, with emphasis on methods that specifically identify antibodies targeting the extracellular domain of AQP1, i.e., those of potential pathogenic role, and (b) the available evidence supporting the pathogenic relevance of AQP1-antibodies in the NMOSD phenotype.
Twenty Years of Anti-Vascular Endothelial Growth Factor Therapeutics in Neovascular Age-Related Macular Degeneration Treatment
Authors: Moon Bo-Hyun , Kim Younghwa , Kim Soo-Young
Published: August 2023

Neovascular age-related macular degeneration (nAMD) is the primary disastrous retinal disease that leads to blindness in the elderly population. In the early 2000s, nAMD resulted in irreversible vision loss and blindness with no available treatment options. However, there have been breakthrough advances in the drug development of anti-angiogenic biological agents over the last two decades. The primary target molecule for treating nAMD is the vascular endothelial growth factor (VEGF), and there are currently several anti-VEGF drugs such as bevacizumab, ranibizumab, and aflibercept, which have made nAMD more manageable than before, thus preventing vision loss. Nevertheless, it should be noted that these anti-VEGF drugs for nAMD treatment are not effective in more than half of the patients, and even those who initially gain visual improvements lose their vision over time, along with potential deterioration in the geography of atrophy. As a result, there have been continuous endeavors to improve anti-VEGF agents through better efficacy, fewer doses, expanded intervals, and additional targets. This review describes past and current anti-VEGF therapeutics used to treat nAMD and outlines future directions to improve the effectiveness and safety of anti-VEGF agents.
Tricuspid Regurgitation (TR) after Implantation of a Cardiac Implantable Electronic Device (CIED)—One-Year Observation of Patients with or without Left Ventricular Dysfunction
Authors: Chodór-Rozwadowska Karolina , Sawicka Magdalena , Morawski Stanisław , Kalarus Zbigniew , Kukulski Tomasz
Published: August 2023

The frequency of tricuspid regurgitation (TR) progression after cardiac implantable electronic devices (CIEDs) implantation varies from 7.2% to 44.7%. TR is associated with increased mortality and hospitalizations due to heart failure (HF) decompensation. The aim of this study was to assess the rate of early TR progression after CIED implantation and the frequency of HF decompensation and mortality. The 101 patients, who received a CIED between March 2020 and October 2021, before the procedure were divided into two groups–one with left ventricle ejection fraction (LVEF) ≥ 40% (n = 60) and one with LVEF < 40% (n = 41). Lead-related tricuspid regurgitation (LRTR) was defined as an increase of TR by at least one grade. The follow-up period was similar between both groups and was on average 13 (12–16) months. In the whole study group, TR progression by one grade was 34.6% and by two or more grades 15.8%. The significant changes in the dynamic of TR degree were as follows before and after implantation: none/trivial TR in group 1 (61.7% vs. 28.3%, p = 0.01) and severe/massive TR in group 2 (0.0% vs. 14.6%, p = 0.03). The groups did not differ from each other in terms of survival from decompensation of HF (18.3% vs. 36.6%, p = 0.70) and survival from death (1.7% vs. 4.9%, p = 0.16). At the one-year follow-up, the baseline LVEF did not affect the survival rate from death or HF decompensation among patients with a progression of TR after CIED implantation. In this study, a progression by one grade was more common in group 1, but the occurrence of severe/massive TR after implantation was more specific for group 2.
Analysis of In Vitro Leukocyte Responses to Biomaterials in the Presence of Antimicrobial Porcine Neutrophil Extract (AMPNE)
Authors: Drzewiecka Beata , Przekora Agata , Dobko Dominika , Kozera Aleksandra , Krać Katarzyna , Nguyen Ngoc Dominika , Fernández-De la Cruz Eric , Wessely-Szponder Joanna
Published: August 2023

Implant insertion can evoke excessive inflammation which disrupts the healing process and potentially leads to complications such as implant rejection. Neutrophils and macrophages play a vital role in the early inflammatory phase of tissue repair, necessitating the study of cellular responses in host–implant interactions. In order to deepen the knowledge about these interactions, the response of neutrophils and macrophages to contact with selected biomaterials was examined in vitro on the basis of secretory response as well as reactive oxygen species/reactive nitrogen species (ROS/RNS) generation. Porcine neutrophils exposed to hydroxyapatite (HA) released more enzymes and generated higher levels of ROS/RNS compared to the control group. The addition of AMPNE diminished these responses. Although the results from porcine cells can provide valuable preliminary data, further validation using human cells or clinical studies would be necessary to fully extrapolate the findings to human medicine. Our study revealed that human neutrophils after contact of with HA increased the production of nitric oxide (NO) (10.00 ± 0.08 vs. control group 3.0 ± 0.11 µM, p < 0.05), while HAP or FAP did not elicit a significant response. Human macrophages cultured with HA produced more superoxide and NO, while HAP or FAP had a minimal effect, and curdlan reduced ROS/RNS generation. The addition of AMPNE to cultures with all biomaterials, except curdlan, reduced neutrophil activity, regardless of the peptides’ origin. These results highlight the potential of antimicrobial peptides in modulating excessive biomaterial/host cell reactions involving neutrophils and macrophages, enhancing our understanding of immune reactions, and suggesting that AMPNE could regulate leukocyte response during implantation.
Unraveling the Interplay of Physical-Chemical Factors Impacting the Carbonation Performance of Recycled Aggregate Concrete
Authors: Pico-Cortés Carlos , Villagrán-Zaccardi Yury
Published: August 2023

Recycled aggregate concrete (RAC) includes recycled concrete aggregates (coarse and/or fine) as substitutes for natural aggregates as an approach to achieving a circular economy. Some concerns remain about its performance, including the carbonation resistance. The higher porosity of recycled concrete aggregates is logically a disadvantage, but the analysis must address many other factors. This paper provides an in-depth examination of recent advances in the carbonation performance of RAC. The emphasis is on factors that influence CO2 diffusion and the carbonation rate, e.g., the replacement ratio, source concrete quality, interfacial transition zone features, residual portlandite content, and porosity. The influences of previous treatments, combined action with supplementary cementitious materials, and loading conditions are also discussed. The replacement ratio has a significant impact on the carbonation performance of concrete, but it is also dependent on other factors. During carbonation, the physical effects of the porosity of the aggregate and the physical–chemical effects of the portlandite content in the adhered mortar are particularly important. The residual portlandite is especially significant because it is the primary hydration product responsible for the alkaline reserve for carbonation and the potential pozzolanic reaction, which are per se competing factors that determine the carbonation rate.
Measurement of Creep Stress Exponent of TC17 Titanium Alloy by Nanoindentation Method at Room Temperature
Authors: Li Fagui , Chen Xiyu , Wang Yuannan , Zhao Guolong , Yang Yinfei
Published: August 2023

The creep stress exponent is commonly employed to characterize the deformation mechanism during the steady-state creep stage, serving as an indicator of creep behavior. The creep phenomenon of high melting point metallic materials is not obvious at room temperature. However, the nanoindentation method proves suitable for investigating the creep properties of metallic materials under such conditions. Consequently, this paper places emphasis on measuring the creep stress exponent of TC17 titanium alloy at room temperature using the load preservation stage of the nanoindentation method with a constant loading rate. In order to investigate the effects of loading rate and maximum load on the experimental results, different loading rates were applied to the diamond Berkovich indenter to reach different maximum loads. The indenter was held under the maximum load for a duration of 360 s, and the relationship between the indentation strain rate and indentation stress during the holding process was used to obtain the creep stress exponent of the material at room temperature. The findings indicate that within the loading rate range of 1.25 to 15 mN/s and maximum load range of 50 to 300 mN, the influence on the experimental results is insignificant. Ultimately, the distribution range of the creep stress exponent for TC17 titanium alloy at room temperature was measured to be 8.524–8.687.
Effect of Tempering Temperature on Hydrogen Embrittlement of SCM440 Tempered Martensitic Steel
Authors: Kim Sang-Gyu , Kim Jae-Yun , Hwang Byoungchul
Published: August 2023

The effect of tempering temperature on the hydrogen embrittlement characteristics of SCM440 tempered martensitic steels was investigated in terms of their microstructure and hydrogen desorption behavior. The microstructures were characterized using scanning and transmission electron microscopy, as well as X-ray diffraction and electron backscattered diffraction analysis. Thermal desorption analysis (TDA) was performed to examine the amount and trapping behavior of hydrogen. The cementite morphology of the SCM440 tempered martensitic steels gradually changed from a long lamellar shape to a segmented short-rod shape with an increasing tempering temperature. A slow strain rate tensile test was conducted after electrochemical hydrogen charging to evaluate the hydrogen embrittlement resistance. The hydrogen embrittlement resistance of the SCM440 tempered martensitic steels increased with an increasing tempering temperature because of the decrease in the fraction of the low-angle grain boundaries and dislocation density. The low-angle grain boundaries and dislocations, which acted as reversible hydrogen trap sites, were critical factors in determining the hydrogen embrittlement resistance, and this was supported by the decreased diffusible hydrogen content as measured by TDA. Fine carbides formed in the steel tempered at a relatively higher temperature acted as irreversible hydrogen trap sites and contributed to improving the hydrogen embrittlement resistance. Our findings can suggest that the tempering temperature of SCM440 tempered martensitic steel plays an important role in determining its hydrogen embrittlement resistance.
Artificial Neural Networks for Predicting the Diameter of Electrospun Nanofibers Synthesized from Solutions/Emulsions of Biopolymers and Oils
Authors: Cuahuizo-Huitzil Guadalupe , Olivares-Xometl Octavio , Eugenia Castro María , Arellanes-Lozada Paulina , Meléndez-Bustamante Francisco J. , Pineda Torres Ivo Humberto , Santacruz-Vázquez Claudia , Santacruz-Vázquez Verónica
Published: August 2023

In the present work, different configurations of nt iartificial neural networks (ANNs) were analyzed in order to predict the experimental diameter of nanofibers produced by means of the electrospinning process and employing polyvinyl alcohol (PVA), PVA/chitosan (CS) and PVA/aloe vera (Av) solutions. In addition, gelatin type A (GT)/alpha-tocopherol (α-TOC), PVA/olive oil (OO), PVA/orange essential oil (OEO), and PVA/anise oil (AO) emulsions were used. The experimental diameters of the nanofibers electrospun from the different tested systems were obtained using scanning electron microscopy (SEM) and ranged from 93.52 nm to 352.1 nm. Of the three studied ANNs, the one that displayed the best prediction results was the one with three hidden layers with the flow rate, voltage, viscosity, and conductivity variables. The calculation error between the experimental and calculated diameters was 3.79%. Additionally, the correlation coefficient (R2) was identified as a function of the ANN configuration, obtaining values of 0.96, 0.98, and 0.98 for one, two, and three hidden layer(s), respectively. It was found that an ANN configuration having more than three hidden layers did not improve the prediction of the experimental diameter of synthesized nanofibers.
Analyzing Quality Measurements for Dimensionality Reduction
Authors: Thrun Michael C. , Märte Julian , Stier Quirin
Published: August 2023

Dimensionality reduction methods can be used to project high-dimensional data into low-dimensional space. If the output space is restricted to two dimensions, the result is a scatter plot whose goal is to present insightful visualizations of distance- and density-based structures. The topological invariance of dimension indicates that the two-dimensional similarities in the scatter plot cannot coercively represent high-dimensional distances. In praxis, projections of several datasets with distance- and density-based structures show a misleading interpretation of the underlying structures. The examples outline that the evaluation of projections remains essential. Here, 19 unsupervised quality measurements (QM) are grouped into semantic classes with the aid of graph theory. We use three representative benchmark datasets to show that QMs fail to evaluate the projections of straightforward structures when common methods such as Principal Component Analysis (PCA), Uniform Manifold Approximation projection, or t-distributed stochastic neighbor embedding (t-SNE) are applied. This work shows that unsupervised QMs are biased towards assumed underlying structures. Based on insights gained from graph theory, we propose a new quality measurement called the Gabriel Classification Error (GCE). This work demonstrates that GCE can make an unbiased evaluation of projections. The GCE is accessible within the R package DR quality available on CRAN.
Specific Learning Disorders: Variation Analysis of 15 Candidate Genes in 9 Multiplex Families
Authors: Calì Francesco , Di Blasi Francesco Domenico , Avola Emanuela , Vinci Mirella , Musumeci Antonino , Gloria Angelo , Greco Donatella , Raciti Daniela Rita , Zagami Alessandro , Rizzo Biagio , Città Santina , Federico Concetta , Vetri Luigi , Saccone Salvatore , Buono Serafino
Published: August 2023

Background and Objectives: Specific Learning Disorder (SLD) is a complex neurobiological disorder characterized by a persistent difficult in reading (dyslexia), written expression (dysgraphia), and mathematics (dyscalculia). The hereditary and genetic component is one of the underlying causes of SLD, but the relationship between genes and the environment should be considered. Several genetic studies were performed in different populations to identify causative genes. Materials and Methods: Here, we show the analysis of 9 multiplex families with at least 2 individuals diagnosed with SLD per family, with a total of 37 persons, 21 of whom are young subjects with SLD, by means of Next-Generation Sequencing (NGS) to identify possible causative mutations in a panel of 15 candidate genes: CCPG1, CYP19A1, DCDC2, DGKI, DIP2A, DYM, GCFC2, KIAA0319, MC5R, MRPL19, NEDD4L, PCNT, PRMT2, ROBO1, and S100B. Results: We detected, in eight families out nine, SNP variants in the DGKI, DIP2A, KIAA0319, and PCNT genes, even if in silico analysis did not show any causative effect on this behavioral condition. In all cases, the mutation was transmitted by one of the two parents, thus excluding the case of de novo mutation. Moreover, the parent carrying the allelic variant transmitted to the children, in six out of seven families, reports language difficulties. Conclusions: Although the present results cannot be considered conclusive due to the limited sample size, the identification of genetic variants in the above genes can provide input for further research on the same, as well as on other genes/mutations, to better understand the genetic basis of this disorder, and from this perspective, to better understand also the neuropsychological and social aspects connected to this disorder, which affects an increasing number of young people.
Muscle Cramps in Outpatients with Liver Diseases in Tokyo, Japan
Authors: Kanda Tatsuo , Sasaki-Tanaka Reina , Matsumoto Naoki , Arima Shuhei , Kanezawa Shini , Honda Masayuki , Totsuka Mai , Ishii Tomotaka , Masuzaki Ryota , Ogawa Masahiro , Yamagami Hiroaki , Kogure Hirofumi
Published: August 2023

Background and Objectives: Muscle cramps are often observed in patients with liver diseases, especially advanced liver fibrosis. The exact prevalence of muscle cramps in outpatients with liver diseases in Japan is unknown. Patients and Methods: This study examined the prevalence of, and therapies for, muscle cramps in outpatients with liver diseases in Tokyo, Japan. A total of 238 outpatients with liver diseases were retrospectively examined. We investigated whether they had muscle cramps using a visual analog scale (VAS) (from 0, none, to 10, strongest), and also investigated their therapies. Results: Muscle cramps were observed in 34 outpatients with liver diseases (14.3%); their mean VAS score was 5.53. A multivariate analysis demonstrated that older age (equal to or older than 66 years) was the only significant factor as-sociated with muscle cramps. The prevalence of muscle cramps among patients with liver diseases seemed not to be higher. The problem was that only 11 (32.4%) of 34 outpatients received therapy for their muscle cramps. Conclusions: Only age is related to muscle cramps, which is rather weak, and it is possible that this common symptom may not be limited to liver disease patients.
Is There a Difference in the Distribution of Mechanoreceptors among the Three Sections of the Anterior Talofibular Ligament?
Authors: Lee Youngkoo , Park Wonseok , Lee Hyerim , Choi Youngsuk , Kim Sunghwan , Yeo Euidong , Lee Hongseop , Jung Kijin , Lee Byungryul , Lee Myoungjin , Kim Woojong
Published: August 2023

Background: We investigated whether the distribution of mechanoreceptors in three sections of the anterior talofibular ligament (ATFL) differed. Methods: The ATFL was obtained from 29 ankles of 21 fresh-frozen cadavers and divided into fibular attachment, mid-ligament, and talar attachment parts. Histologically, mechanoreceptors were classified as Ruffini (type I), Vater–Pacini (type II), Golgi–Mazzoni (type III), and free nerve ending corpuscles (type IV); the presence of these mechanoreceptors was compared among the three ATFL sections. Results: Type I mechanoreceptors were significantly more numerous than the other receptor types. Comparing the three sections of the ATFL, the number of type I mechanoreceptors differed significantly between the mid-ligament and fibular attachment (p = 0.006), while the number of type III mechanoreceptors differed significantly between the talar and fibular attachments (p = 0.005) and between the mid-ligament and talar attachment (p = 0.011). Conclusions: The four types of mechanoreceptors were distributed differently among the three sections of the ATFL. Type I mechanoreceptors were more numerous in all sections compared to the other receptors.
Breakthrough in Silicon Photonics Technology in Telecommunications, Biosensing, and Gas Sensing
Authors: Shahbaz Muhammad , Butt Muhammad A. , Piramidowicz Ryszard
Published: August 2023

Silicon photonics has been an area of active research and development. Researchers have been working on enhancing the integration density and intricacy of silicon photonic circuits. This involves the development of advanced fabrication techniques and novel designs to enable more functionalities on a single chip, leading to higher performance and more efficient systems. In this review, we aim to provide a brief overview of the recent advancements in silicon photonic devices employed for telecommunication and sensing (biosensing and gas sensing) applications.
A Frequency-Dependent Dynamic Electric–Mechanical Network for Thin-Wafer Piezoelectric Transducers Polarized in the Thickness Direction: Physical Model and Experimental Confirmation
Authors: Fa Lin , Liu Dongning , Gong Hong , Chen Wenhui , Zhang Yandong , Wang Yimei , Liang Rui , Wang Baoni , Shi Guiquan , Fang Xiangrong , Li Yuxia , Zhao Meishan
Published: August 2023

This paper is concerned with electric–acoustic/acoustic–electric conversions of thin-wafer piezoelectric transducers polarized in the thickness direction. By introducing two mechanical components with frequency-dependent values, i.e., radiation resistance and radiation mass, into the equivalent circuit of the thin-wafer piezoelectric transducer, we established a frequency-dependent dynamic mechanic-electric equivalent network with four terminals for an arbitrary given frequency, an enhancement from the conventional circuit networks. We derived the analytic expressions of its electric–acoustic and acoustic–electric conversion impulse responses using the four-terminal equivalent circuit to replace the traditional six-terminal equivalent circuit for a thin-wafer transducer with harmonic vibrational motion. For multifrequency electrical/acoustic signals acting on the transducer, we established parallel electric–acoustic/acoustic–electric conversion transmission networks. These two transmission network models have simple structures and clear physical and mathematical descriptions of thin-wafer transducers for electric–acoustic/acoustic–electric conversion when excited by a multifrequency electric/acoustic signal wavelet. The calculated results showed that the transducer’s center frequency shift relates to its mechanical load and vibration state. The method reported in this paper can be applied to conventional-sized and small-sized piezoelectric transducers with universal applicability.
Optimising Surface Roughness and Density in Titanium Fabrication via Laser Powder Bed Fusion
Authors: Hassanin Hany , El-Sayed Mahmoud Ahmed , Ahmadein Mahmoud , Alsaleh Naser A. , Ataya Sabbah , Ahmed Mohamed M. Z. , Essa Khamis
Published: August 2023

The Ti6Al4V alloy has many advantages, such as being lightweight, formal, and resistant to corrosion. This makes it highly desirable for various applications, especially in the aerospace industry. Laser Powder Bed Fusion (LPBF) is a technique that allows for the production of detailed and unique parts with great flexibility in design. However, there are challenges when it comes to achieving high-quality surfaces and porosity formation in the material, which limits the wider use of LPBF. To tackle these challenges, this study uses statistical techniques called Design of Experiments (DoE) and Analysis of Variance (ANOVA) to investigate and optimise the process parameters of LPBF for making Ti6Al4V components with improved density and surface finish. The parameters examined in this study are laser power, laser scan speed, and hatch space. The optimisation study results show that using specific laser settings, like a laser power of 175 W, a laser scan speed of 1914 mm/s, and a hatch space of 53 µm, produces Ti6Al4V parts with a high relative density of 99.54% and low top and side surface roughness of 2.6 µm and 4.3 µm, respectively. This promising outcome demonstrates the practicality of optimising Ti6Al4V and other metal materials for a wide range of applications, thereby overcoming existing limitations and further expanding the potential of LPBF while minimising inherent process issues.