bluuebunny/crossref_metadata_2025 huggingface dataset:
Seedless Cu Electroplating on Ru-W Thin Films for Metallisation of Advanced Interconnects
Authors: Santos Rúben F. , Oliveira Bruno M. C. , Savaris Liliane C. G. , Ferreira Paulo J. , Vieira Manuel F.
Published: February 2022

For decades, Ta/TaN has been the industry standard for a diffusion barrier against Cu in interconnect metallisation. The continuous miniaturisation of transistors and interconnects into the nanoscale are pushing conventional materials to their physical limits and creating the need to replace them. Binary metallic systems, such as Ru-W, have attracted considerable attention as possible replacements due to a combination of electrical and diffusion barrier properties and the capability of direct Cu electroplating. The process of Cu electrodeposition on Ru-W is of fundamental importance in order to create thin, continuous, and adherent films for advanced interconnect metallisation. This work investigates the effects of the current density and application method on the electro-crystallisation behaviour of Cu. The film structure, morphology, and chemical composition were assessed by digital microscopy, atomic force microscopy, scanning and transmission electron microscopies, energy-dispersive X-ray spectroscopy, and X-ray diffraction. The results show that it was possible to form a thin Cu film on Ru-W with interfacial continuity for current densities higher than 5 mA·cm−2; however, the substrate regions around large Cu particles remained uncovered. Pulse-reverse current application appears to be more beneficial than direct current as it decreased the average Cu particle size.
CO2 Absorption Mechanism by the Deep Eutectic Solvents Formed by Monoethanolamine-Based Protic Ionic Liquid and Ethylene Glycol
Authors: Cheng Jinyu , Wu Congyi , Gao Weiji , Li Haoyuan , Ma Yanlong , Liu Shiyu , Yang Dezhong
Published: February 2022

Deep eutectic solvents (DESs) have been widely used to capture CO2 in recent years. Understanding CO2 mechanisms by DESs is crucial to the design of efficient DESs for carbon capture. In this work, we studied the CO2 absorption mechanism by DESs based on ethylene glycol (EG) and protic ionic liquid ([MEAH][Im]), formed by monoethanolamine (MEA) with imidazole (Im). The interactions between CO2 and DESs [MEAH][Im]-EG (1:3) are investigated thoroughly by applying 1H and 13 C nuclear magnetic resonance (NMR), 2-D NMR, and Fourier-transform infrared (FTIR) techniques. Surprisingly, the results indicate that CO2 not only binds to the amine group of MEA but also reacts with the deprotonated EG, yielding carbamate and carbonate species, respectively. The reaction mechanism between CO2 and DESs is proposed, which includes two pathways. One pathway is the deprotonation of the [MEAH]+ cation by the [Im]− anion, resulting in the formation of neutral molecule MEA, which then reacts with CO2 to form a carbamate species. In the other pathway, EG is deprotonated by the [Im]−, and then the deprotonated EG, HO-CH2-CH2-O−, binds with CO2 to form a carbonate species. The absorption mechanism found by this work is different from those of other DESs formed by protic ionic liquids and EG, and we believe the new insights into the interactions between CO2 and DESs will be beneficial to the design and applications of DESs for carbon capture in the future.
The Potential Effect of Lidocaine, Ropivacaine, Levobupivacaine and Morphine on Breast Cancer Pre-Clinical Models: A Systematic Review
Authors: Matos Ana , Marques Inês , Pires Ana , Valentim Ana , Abrantes Ana , Botelho Maria
Published: February 2022

Breast cancer (BC) is one of the most common types of cancer and the second leading cause of death in women. Local anaesthetics (LAs) and opioids have been shown to influence cancer progression and metastasis formation in several pre-clinical studies. However, their effects do not seem to promote consensus. A systematic review was conducted using the databases Medline (via PubMed), Scopus, and Web of Science (2010 to December 2021). Search terms included “lidocaine”, “ropivacaine”, “levobupivacaine”, “morphine”, “methadone”, “breast cancer”, “breast carcinoma” and “breast neoplasms” in diverse combinations. The search yielded a total of 784 abstracts for initial review, 23 of which met the inclusion criteria. Here we summarise recent studies on the effect of analgesics and LAs on BC cell lines and animal models and in combination with other treatment regimens. The results suggest that local anaesthetics have anti-tumorigenic properties, hence their clinical application holds therapeutic potential. Regarding morphine, the findings are conflicting, but this opioid appears to be a tumour-promoting agent. Methadone-related results are scarce. Additional research is clearly required to further study the mechanisms underlying the controversial effects of each analgesic or LA to establish the implications upon the outcome and prognosis of BC patients’ treatment.
Calcium Signalling in Breast Cancer Associated Bone Pain
Authors: Bortolin Andrea , Neto Estrela , Lamghari Meriem
Published: February 2022

Calcium (Ca2+) is involved as a signalling mediator in a broad variety of physiological processes. Some of the fastest responses in human body like neuronal action potential firing, to the slowest gene transcriptional regulation processes are controlled by pathways involving calcium signalling. Under pathological conditions these mechanisms are also involved in tumoral cells reprogramming, resulting in the altered expression of genes associated with cell proliferation, metastatisation and homing to the secondary metastatic site. On the other hand, calcium exerts a central function in nociception, from cues sensing in distal neurons, to signal modulation and interpretation in the central nervous system leading, in pathological conditions, to hyperalgesia, allodynia and pain chronicization. It is well known the relationship between cancer and pain when tumoral metastatic cells settle in the bones, especially in late breast cancer stage, where they alter the bone micro-environment leading to bone lesions and resulting in pain refractory to the conventional analgesic therapies. The purpose of this review is to address the Ca2+ signalling mechanisms involved in cancer cell metastatisation as well as the function of the same signalling tools in pain regulation and transmission. Finally, the possible interactions between these two cells types cohabiting the same Ca2+ rich environment will be further explored attempting to highlight new possible therapeutical targets.
Mitochondrial-Targeted Therapy for Doxorubicin-Induced Cardiotoxicity
Authors: Wu Bin Bin , Leung Kam Tong , Poon Ellen Ngar-Yun
Published: February 2022

Anthracyclines, such as doxorubicin, are effective chemotherapeutic agents for the treatment of cancer, but their clinical use is associated with severe and potentially life-threatening cardiotoxicity. Despite decades of research, treatment options remain limited. The mitochondria is commonly considered to be the main target of doxorubicin and mitochondrial dysfunction is the hallmark of doxorubicin-induced cardiotoxicity. Here, we review the pathogenic mechanisms of doxorubicin-induced cardiotoxicity and present an update on cardioprotective strategies for this disorder. Specifically, we focus on strategies that can protect the mitochondria and cover different therapeutic modalities encompassing small molecules, post-transcriptional regulators, and mitochondrial transfer. We also discuss the shortcomings of existing models of doxorubicin-induced cardiotoxicity and explore advances in the use of human pluripotent stem cell derived cardiomyocytes as a platform to facilitate the identification of novel treatments against this disorder.
ERAP2 Inhibition Induces Cell-Surface Presentation by MOLT-4 Leukemia Cancer Cells of Many Novel and Potentially Antigenic Peptides
Authors: Temponeras Ioannis , Stamatakis George , Samiotaki Martina , Georgiadis Dimitris , Pratsinis Harris , Panayotou George , Stratikos Efstratios
Published: February 2022

Recent studies have linked the activity of ER aminopeptidase 2 (ERAP2) to increased efficacy of immune-checkpoint inhibitor cancer immunotherapy, suggesting that pharmacological inhibition of ERAP2 could have important therapeutic implications. To explore the effects of ERAP2 inhibition on the immunopeptidome of cancer cells, we treated MOLT-4 T lymphoblast leukemia cells with a recently developed selective ERAP2 inhibitor, isolated Major Histocompatibility class I molecules (MHCI), and sequenced bound peptides by liquid chromatography tandem mass spectrometry. Inhibitor treatment induced significant shifts on the immunopeptidome so that more than 20% of detected peptides were either novel or significantly upregulated. Most of the inhibitor-induced peptides were 9mers and had sequence motifs and predicted affinity consistent with being optimal ligands for at least one of the MHCI alleles carried by MOLT-4 cells. Such inhibitor-induced peptides could serve as triggers for novel cytotoxic responses against cancer cells and synergize with the therapeutic effect of immune-checkpoint inhibitors.
Functional Transient Receptor Potential Ankyrin 1 and Vanilloid 1 Ion Channels Are Overexpressed in Human Oral Squamous Cell Carcinoma
Authors: Kiss Fruzsina , Kormos Viktória , Szőke Éva , Kecskés Angéla , Tóth Norbert , Steib Anita , Szállási Árpád , Scheich Bálint , Gaszner Balázs , Kun József , Fülöp Gábor , Pohóczky Krisztina , Helyes Zsuzsanna
Published: February 2022

Oral squamous cell carcinoma (OSCC) is a common cancer with poor prognosis. Transient Receptor Potential Ankyrin 1 (TRPA1) and Vanilloid 1 (TRPV1) receptors are non-selective cation channels expressed on primary sensory neurons and epithelial and immune cells. TRPV1 mRNA and immunopositivity, as well as TRPA1-like immunoreactivity upregulation, were demonstrated in OSCC, but selectivity problems with the antibodies still raise questions and their functional relevance is unclear. Therefore, here, we investigated TRPA1 and TRPV1 expressions in OSCC and analyzed their functions. TRPA1 and TRPV1 mRNA were determined by RNAscope in situ hybridization and qPCR. Radioactive 45Ca2+ uptake and ATP-based luminescence indicating cell viability were measured in PE/CA-PJ41 cells in response to the TRPA1 agonist allyl-isothiocyanate (AITC) and TRPV1 agonist capsaicin to determine receptor function. Both TRPA1 and TRPV1 mRNA are expressed in the squamous epithelium of the human oral mucosa and in PE/CA-PJ41 cells, and their expressions are significantly upregulated in OSCC compared to healthy mucosa. TRPA1 and TRPV1 activation (100 µM AITC, 100 nM capsaicin) induced 45Ca2+-influx into PE/CA-PJ41 cells. Both AITC (10 nM–5 µM) and capsaicin (100 nM–45 µM) reduced cell viability, reaching significant decrease at 100 nM AITC and 45 µM capsaicin. We provide the first evidence for the presence of non-neuronal TRPA1 receptor in the OSCC and confirm the expression of TRPV1 channel. These channels are functionally active and might regulate cancer cell viability.
Monitoring Temporal Trends in Internet Searches for “Ticks” across Europe by Google Trends: Tick–Human Interaction or General Interest?
Authors: Jensen Per M. , Danielsen Finn , Skarphedinsson Sigurdur
Published: February 2022

Monitoring vector–human interaction is pivotal for assessing potential transmission rates of vector borne diseases and their associated public health impact. People often seek information following an insect bite in order to identify hematophagous arthropods, which in recent years often is done using Internet resources. Through this activity, a record of net searches is generated, which include information that reflect local human–arthropod interaction, e.g., for the common tick (Ixodes ricinus) in European countries. Such records could in principle provide low cost real-time monitoring data, if indeed Internet search activities adequately reflect tick–human interaction. We here explore Google Trends records for within-year and between-year trends, for four different Danish search terms for “tick(s)”. We further assess the relationship between monthly search-frequencies and local weather conditions (temperatures and precipitation from 2007 to 2016) in nine European countries. Our findings point to significant limitations in the records due to changes in search-term preferences over the given years. However, the seasonal dynamics are comparable among search-terms. Moreover, the seasonal pattern in search terms vary across Europe in tune with changes in temperature and precipitation. We conclude that, the within-year variation for given search-terms provide credible information, which systematically vary with local weather patterns. We are not convinced that these records merely reflect general interest. It will, however, require a more in-depth analysis by researchers that have specific insight into local language practices to fully assess the strength and weaknesses of this approach
Poor Host Status of Australian Finger Lime, Citrus australasica, to Ceratitis capitata, Zeugodacus cucurbitae, and Bactrocera dorsalis (Diptera: Tephritidae) in Hawai’i
Authors: Follett Peter A. , Asmus Glenn , Hamilton Lindsey J.
Published: February 2022

We examined the host status of the Australian finger lime, Citrus australiasica F. Muell. (Rutaceae), to Hawai’i’s tephritid fruit fly pests using laboratory and field studies. In high-density (500 flies, 1:1 males and females) no-choice cage exposures (25 × 25 × 25-cm cage size), both undamaged and punctured finger limes were infested by Ceratitis capitata and Zeugodacuscucurbitae at a low rate compared to papaya controls, whereas Bactrocera dorsalis did not infest undamaged fruit, suggesting finger lime is a nonhost. In low-density (50 females) no-choice cage exposures, C. capitata and Z. cucurbitae readily oviposited in undamaged fruit but individuals rarely developed to the pupal or adult stage. For C. capitata, 274 finger limes exposed to 2000 gravid females, which laid an estimated 14,384 eggs, produced two pupae and no adults. For Z. cucurbitae, 299 fruit exposed to 2000 gravid females, which laid an estimated 4484 eggs, produced four pupae and one adult. Field sampling of undamaged fruit from the tree and off the ground from commercial farms produced five C. capitata pupa and one adult from 1119 fruit, for an infestation rate of 0.05 flies per kilogram of fruit; field collections found no natural Z. cucurbitae or B. dorsalis infestation, but the number of fruit available was too low to demonstrate nonhost status with a high degree of confidence.
Shoaling Wave Shape Estimates from Field Observations and Derived Bedload Sediment Rates
Authors: Kalra Tarandeep S. , Suttles Steve E. , Sherwood Christopher R. , Warner John C. , Aretxabaleta Alfredo L. , Leavitt Gibson R.
Published: February 2022

The shoaling transformation from generally linear deep-water waves to asymmetric shallow-water waves modifies wave shapes and causes near-bed orbital velocities to become asymmetrical, contributing to net sediment transport. In this work, we used two methods to estimate the asymmetric wave shape from data at three sites. The first method converted wave measurements made at the surface to idealized near-bottom wave-orbital velocities using a set of empirical equations: the “parameterized” waveforms. The second method involved direct measurements of velocities and pressure made near the seabed: the “direct” waveforms. Estimates from the two methods were well correlated at all three sites (Pearson’s correlation coefficient greater than 0.85). Both methods were used to drive bedload-transport calculations that accounted for asymmetric waves, and the results were compared with a traditional excess-stress formulation and field estimates of bedload transport derived from ripple migration rates based on sonar imagery. The cumulative bedload transport from the parameterized waveform was 25% greater than the direct waveform, mainly because the parameterized waveform did not account for negative skewness. Calculated transport rates were comparable to rates estimated from ripple migration except during the largest event, when calculated rates were as much as 100 times greater, which occurred during high period waves.
Philip Pullman and Spiritual Quest
Authors: Boucher Geoff M. , Devonport-Ralph Charlotte
Published: February 2022

The polarized initial reception of Philip Pullman as a “new atheist” has gradually yielded to more nuanced scholarly positionings of his work as inspired by a heterodox, even “heretical,” Christianity. But in his new series, Pullman responds decisively to both “new atheist” and “heterodox Christian” interpretations, while widening the scope of his critical representations beyond Christian—indeed, beyond Abrahamic—religion. What emerges in the completed books of the incomplete new series, The Book of Dust, is a “secret commonwealth” of supernatural beings inhabiting multiple universes. These are all manifestations of Dust, the spiritual sentience of matter itself, which provides the basis for mystical visions and shamanistic beliefs, as well as religious orthodoxies. Rejecting the latter for the former, the second book in particular, The Secret Commonwealth, suggests an endorsement of spiritual quest. To motivate acceptance of this interpretation, we begin by reviewing the critical reception of His Dark Materials, especially in relation to its theological implications. After that, we turn to the representation of reductionist positions in The Book of Dust, especially the authors presented in The Secret Commonwealth, Gottfried Brande and Simon Talbot. Then, we investigate the representation of the Abrahamic religions in that work, intrigued less by the obvious parallels between Pullman’s imaginary religions and Christianity and Islam, than by his positive representation of mysticism. Finally, we examine his representations of shamanism and animism, soul belief and hermetic doctrines, and his allusions to Zoroastrianism, before summing up. Pullman is an a-theist in the sense of being without a god, not in the post-Enlightenment sense of a rejection of the supernatural/spiritual. His imaginary universe celebrates spiritual quest and ontological multiplicity, against all forms of speculative closure.
Influence of Blockchain Technology in Manufacturing Supply Chain and Logistics
Authors: Raja Santhi Abirami , Muthuswamy Padmakumar
Published: February 2022

Background: Blockchain is a digitally managed, distributed, and decentralized ledger used to record transactions in an immutable format. Its characteristics in providing trust, transparency, and traceability make it attractive for applications where transactions are involved. Originally intended to support financial transactions, the technology has gained attention even in non-financial sectors such as health care, manufacturing, retail, and government services. Methods: For centuries, the various functions of manufacturing industries have worked based on the relationship and trust that they have with their upstream and downstream stakeholders. In addition, as the conventional factories are growing into giant gigafactories, the participation of several intermediaries further complicates the supply chain and logistics operations. Hence, the article aims to provide a comprehensive overview of the role of blockchain technology in addressing supply chain and logistics-related challenges by analyzing, organizing, and reviewing the literature. Results: The study shows that blockchain technology can transform the supply chain and logistics into secure, agile, trusted, and transparent functions. A conceptualized application scenario demonstrates the benefits of blockchain technology in providing provenance and traceability to critical products. Conclusions: In particular, a private or permissioned blockchain is suitable for multi-organizational businesses such as supply chain and logistics. In addition, IoT-blockchain integration, smart contracts, and asset tracking has immense benefits in the future.
Investigation into the Electrohydraulic Synchronous Motion Control of a Thrust System for a Tunnel Boring Machine
Authors: Wu Weiqiang , Gong Guofang , Huan Quan , Zhou Xinghai , Chen Yuxi , Peng Xiongbin
Published: February 2022

The thrust system of a tunnel boring machine plays a crucial role by driving the machine ahead and supporting the gripper shoes stably. A thrust hydraulic control system, assembled with a proportional flow control valve and a pressure relief valve, is established with system operating parameters. The mathematical model of a thrust electrohydraulic system is presented. To improve the control characteristics of the thrust system, a self-tuning fuzzy PID controller was introduced in synchronization motion control situations. To attain the best control parameters, three synchronization motion control systems were used to control the thrust propel cylinders. Tests on a Ø2.5 m scaled TBM test rig were carried out to verify the capabilities of the ISCS, SRSCS and CRSCS. Comparative tests were conducted, and the results showed that the thrust system adopting SRSCS achieved the least oscillation and the quickest response. The steady-state displacement error decreased by about 33.3% in contrast to the ISCS and CRSCS.
Optimal Management of Energy Consumption in an Autonomous Power System Considering Alternative Energy Sources
Authors: Manusov Vadim , Beryozkina Svetlana , Nazarov Muso , Safaraliev Murodbek , Zicmane Inga , Matrenin Pavel , Ghulomzoda Anvari
Published: February 2022

This work aims to analyze and manage the optimal power consumption of the autonomous power system within the Pamir region of Republic of Tajikistan, based on renewable energy sources. The task is solved through linear programming methods, production rules and mathematical modeling of power consumption modes by generating consumers. It is assumed that power consumers in the considered region have an opportunity to independently cover energy shortage by installing additional generating energy sources. The objective function is to minimize the financial expenses for own power consumption, and to maximize them from both the export and redistribution of power flows. In this study, the optimal ratio of power generation by alternative sources from daily power consumption for winter was established to be hydroelectric power plants (94.8%), wind power plant (3.8%), solar photovoltaic power plant (0.5%) and energy storage (0.8%); while it is not required in summer due to the ability to ensure the balance of energy by hydroelectric power plants. As a result, each generating consumer can independently minimize their power consumption and maximize profit from the energy exchange with other consumers, depending on the selected energy sources, thus becoming a good example of carbon-free energy usage at the micro- and mini-grid level.
Utilization of the Revised American College of Rheumatology (rACR) Scoring to Avoid Unnecessary Temporal Artery Biopsies—A Case Series
Authors: Mansoor Tayyaub , Lynch Noel P. , Rifai Hicham , Hamlin Sean , Moneley Darragh
Published: February 2022

Introduction: The American College of Rheumatology (ACR) criteria, and more recently the revised ACR criteria (rACR), are a scoring system developed to aid in the diagnosis of giant cell arteritis (GCA). Our aim was to investigate the value of the non-biopsy criteria of the original ACR criteria and rACR criteria to predict GCA, and investigate the utilization of such scores to avoid biopsy when a very high or very low likelihood of a positive temporal artery biopsy TAB was predicted. Method: We conducted a retrospective cohort study of 59 patients undergoing TAB from 2013 to 2017 in Beaumont Hospital, a tertiary referral centre in Dublin, Ireland. Demographic data, biochemical results, presenting features, and histology results were collected and collated. Results: Data were analysed from 53 patients and ACR scores were compiled. Seventeen scored < 3 and thirty-six scored 3–5. All 11 positive biopsies were in the 3–5 score range. Forty-five patients were analysed with rACR scores. Eight were excluded due to not meeting the inclusion criteria. Of the 11 positive biopsies, 2 were in the 3–4 score range, and 9 were in the ≥5 score range. In the ACR method, 36% of all biopsies scored as low-risk pre-biopsy. In the rACR method, 84.4% of all biopsies scored in the low- and intermediate-risk group pre-biopsy and 15.6% of all biopsies scored in the high-risk group pre-biopsy. Conclusions: This study illustrates the potential value of the rACR scoring system as a useful tool to categorize patients according to risk with a view to avoiding unnecessary TAB. The data suggest that a TAB has a helpful role in low- and intermediate-risk groups but is of minimal benefit in the high-risk group.
Phylogenetic Studies and Metabolite Analysis of Sticta Species from Colombia and Chile by Ultra-High Performance Liquid Chromatography-High Resolution-Q-Orbitrap-Mass Spectrometry
Authors: Albornoz Laura , Torres-Benítez Alfredo , Moreno-Palacios Miguel , Simirgiotis Mario J. , Montoya-Serrano Saúl A. , Sepulveda Beatriz , Stashenko Elena , García-Beltrán Olimpo , Areche Carlos
Published: February 2022

Eleven species of lichens of the genus Sticta, ten of which were collected in Colombia (S. pseudosylvatica S. luteocyphellata S. cf. andina S. cf. hypoglabra, S. cordillerana, S. cf. gyalocarpa S. leucoblepharis, S. parahumboldtii S. impressula, S. ocaniensis) and one collected in Chile (S. lineariloba), were analyzed for the first time using hyphenated liquid chromatography with high-resolution mass spectrometry. In the metabolomic analysis, a total of 189 peaks were tentatively detected; the analyses were divided in five (5) groups of compounds comprising lipids, small phenolic compounds, saturated acids, terpenes, and typical phenolic lichen compounds such as depsides, depsidones and anthraquinones. The metabolome profiles of these eleven species are important since some compounds were identified as chemical markers for the fast identification of Sticta lichens for the first time. Finally, the usefulness of chemical compounds in comparison to traditional morphological traits to the study of ancestor–descendant relationships in the genus was assessed. Chemical and morphological consensus trees were not consistent with each other and recovered different relationships between taxa.
A Piece of the Puzzle—Possible Mechanisms for Why Low Dietary EPA and DHA Cause Hepatic Lipid Accumulation in Atlantic Salmon (Salmo salar)
Authors: Hundal Bjørg Kristine , Lutfi Esmail , Sigholt Trygve , Rosenlund Grethe , Liland Nina Sylvia , Glencross Brett , Sissener Nini Hedberg
Published: February 2022

The present study aimed at elucidating the effects of graded levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on the hepatic metabolic health of Atlantic salmon reared in sea cages. Diets containing 10, 13, 16 and 35 g/kg EPA + DHA (designated diets 1.0, 1.3, 1.6 and 3.5, respectively) were fed in triplicate through a full production cycle from an average starting weight of 275 g to slaughter size (~5 kg). Feeding low dietary EPA + DHA altered the hepatic energy metabolism, evidenced by reductions in tricarboxylic acid cycle intermediates originating from β-oxidation, which was compensated by elevated activity in alternative energy pathways (pentose phosphate pathway, branched chain amino acid catabolism and creatine metabolism). Increases in various acylcarnitines in the liver supported this and indicates issues with lipid metabolism (mitochondrial β-oxidation). Problems using lipids for energy in the lower EPA + DHA groups line up well with observed increases in liver lipids in these fish. It also aligns with the growth data, where fish fed the highest EPA + DHA grew better than the other groups. The study showed that diets 1.0 and 1.3 were insufficient for maintaining good liver metabolic health. However, diet 3.5 was significantly better than diet 1.6, indicating that diet 1.6 might also be suboptimal.
Editorial for the Special Issue: “Antimicrobial Resistance and Molecular Tracing of Foodborne Pathogens”
Authors: Hammerl Jens André
Published: February 2022

Foodborne pathogens are a major cause of diarrheal disease worldwide, but also constitute a severe threat for the spread of antimicrobial-resistant bacteria from livestock via food products to humans [...]
Detection of mcr-1 Gene in Undefined Vibrio Species Isolated from Clams
Authors: Valdez Christian , Costa Cátia , Simões Marco , de Carvalho Carla C. C. R. , Baptista Teresa , Campos Maria J.
Published: February 2022

The increase of antimicrobial resistant strains is leading to an emerging threat to public health. Pathogenic Vibrio are responsible for human and animal illness. The Enterobacteriaceae family includes microorganisms that affect humans, causing several infections. One of the main causes of human infection is related to the ingestion of undercooked seafood. Due to their filter-feeding habit, marine invertebrates, such as clams, are known to be a natural reservoir of specific microbial communities. In the present study, Vibrionaceae and coliforms microorganisms were isolated from clams. A microbial susceptibility test was performed using the disk diffusion method. From 43 presumptive Vibrio spp. and 17 coliforms, three Vibrio spp. with MICs to colistin >512 mg L−1 were found. From the 23 antimicrobial resistance genes investigated, only the three isolates that showed phenotypic resistance to colistin contained the mcr-1 gene. Genotypic analysis for virulence genes in EB07V indicated chiA gene presence. The results from the plasmid cure and transformation showed that the resistance is chromosomally mediated. Biochemical analysis and MLSA, on the basis of four protein-coding gene sequences (recA, rpoB, groEL and dnaJ), grouped the isolates into the genus Vibrio but distinguished them as different from any known Vibrio spp.
Homo-Acetogens: Their Metabolism and Competitive Relationship with Hydrogenotrophic Methanogens
Authors: Karekar Supriya , Stefanini Renan , Ahring Birgitte
Published: February 2022

Homo-acetogens are microbes that have the ability to grow on gaseous substrates such as H2/CO2/CO and produce acetic acid as the main product of their metabolism through a metabolic process called reductive acetogenesis. These acetogens are dispersed in nature and are found to grow in various biotopes on land, water and sediments. They are also commonly found in the gastro-intestinal track of herbivores that rely on a symbiotic relationship with microbes in order to breakdown lignocellulosic biomass to provide the animal with nutrients and energy. For this motive, the fermentation scheme that occurs in the rumen has been described equivalent to a consolidated bioprocessing fermentation for the production of bioproducts derived from livestock. This paper reviews current knowledge of homo-acetogenesis and its potential to improve efficiency in the rumen for production of bioproducts by replacing methanogens, the principal H2-scavengers in the rumen, thus serving as a form of carbon sink by deviating the formation of methane into bioproducts. In this review, we discuss the main strategies employed by the livestock industry to achieve methanogenesis inhibition, and also explore homo-acetogenic microorganisms and evaluate the members for potential traits and characteristics that may favor competitive advantage over methanogenesis, making them prospective candidates for competing with methanogens in ruminant animals.
Development of New Technologies for Risk Identification of Schistosomiasis Transmission in China
Authors: Shi Liang , Zhang Jian-Feng , Li Wei , Yang Kun
Published: February 2022

Schistosomiasis is serious parasitic disease with an estimated global prevalence of active infections of more than 190 million. Accurate methods for the assessment of schistosomiasis risk are crucial for schistosomiasis prevention and control in China. Traditional approaches to the identification of epidemiological risk factors include pathogen biology, immunology, imaging, and molecular biology techniques. Identification of schistosomiasis risk has been revolutionized by the advent of computer network communication technologies, including 3S, mathematical modeling, big data, and artificial intelligence (AI). In this review, we analyze the development of traditional and new technologies for risk identification of schistosomiasis transmission in China. New technologies allow for the integration of environmental and socio-economic factors for accurate prediction of the risk population and regions. The combination of traditional and new techniques provides a foundation for the development of more effective approaches to accelerate the process of schistosomiasis elimination.
Magnolol and Luteolin Inhibition of α-Glucosidase Activity: Kinetics and Type of Interaction Detected by In Vitro and In Silico Studies
Authors: Djeujo Francine Medjiofack , Ragazzi Eugenio , Urettini Miriana , Sauro Beatrice , Cichero Elena , Tonelli Michele , Froldi Guglielmina
Published: February 2022

Magnolol and luteolin are two natural compounds recognized in several medicinal plants widely used in traditional medicine, including type 2 diabetes mellitus. This research aimed to determine the inhibitory activity of magnolol and luteolin on α-glucosidase activity. Their biological profile was studied by multispectroscopic methods along with inhibitory kinetic analysis and computational experiments. Magnolol and luteolin decreased the enzymatic activity in a concentration-dependent manner. With 0.075 µM α-glucosidase, the IC50 values were similar for both compounds (~ 32 µM) and significantly lower than for acarbose (815 μM). Magnolol showed a mixed-type antagonism, while luteolin showed a non-competitive inhibition mechanism. Thermodynamic parameters suggested that the binding of magnolol was predominantly sustained by hydrophobic interactions, while luteolin mainly exploited van der Waals contacts and hydrogen bonds. Synchronous fluorescence revealed that magnolol interacted with the target, influencing the microenvironment around tyrosine residues, and circular dichroism explained a rearrangement of the secondary structure of α-glucosidase from the initial α-helix to the final conformation enriched with β-sheet and random coil. Docking studies provided support for the experimental results. Altogether, the data propose magnolol, for the first time, as a potential α-glucosidase inhibitor and add further evidence to the inhibitory role of luteolin.
Structural Analysis and Optimization of Heavy Vehicle Chassis Using Aluminium P100/6061 Al and Al GA 7-230 MMC
Authors: Agarwal Abhishek , Mthembu Linda
Published: February 2022

A chassis is one of the vital parts of a heavy motor vehicle, which provides rigidity to the vehicle and improves structural stability and rigidity for accurate handling. The design and material of a chassis structure significantly affects its strength and weight. Optimization techniques can be used in systematic design improvement of chassis to meet industry requirements. The current research is intended to optimize the design of chassis using the Box–Behnken design scheme and the material tested is P100/6061 Al and Al GA 7-230 MMC. Different design points were generated using the design of the experiments. Equivalent stress, deformation and mass were evaluated for each design point. The variable selected for optimization using the Box–Behnken scheme was cross member width. The CAD modelling and FE simulation of the heavy motor vehicle chassis were conducted using ANSYS software. From the optimization conducted on the chassis design, response surface plots of equivalent stress, deformation and mass were generated, which enabled to determine the range of dimensions for which these parameters are maximum or minimum. The sensitivity plots of different variables were generated, which has shown that cross member 2’s width has a maximum effect on equivalent stress and cross member 3’s width has a minimum effect on equivalent stress, whereas for total deformation, cross member 3 shows the maximum sensitivity percentage, which signifies that cross member 3 has the maximum effect on total deformation, and vice versa. The use of the aluminium metal matrix composites P100/6061 Al and Al GA 7-230 MMC aided to reduce the weight of the chassis by 68% and 70%, respectively, without much reduction in the strength of the chassis.
Acknowledgment to Reviewers of Processes in 2021
Authors:   Processes Editorial Office
Published: February 2022

Rigorous peer-reviews are the basis of high-quality academic publishing [...]
Study on Mechanical Properties of Cement-Improved Frozen Soil under Uniaxial Compression Based on Discrete Element Method
Authors: Ding Fei , Song Lei , Yue Fengtian
Published: February 2022

Taking cement-improved frozen soil as the research object, this paper, based on uniaxial unconfined compressive tests of improved-frozen soil under the conditions of different cement contents (6%, 12%, 18%) and curing ages (7 d, 14 d, 28 d), analyzed the results and probed the relationship between the strength and elastic modulus of cement-improved frozen soil and cement content and curing age. In combination with laboratory test results, numerical simulations were set with the PFC3D group, building on the samples with 6% and 18% cement content at 14 days of curing, respectively, and the mesoscopic parameter values of the two different amounts were calibrated, which proved the simulation of cement with PFC3D reliable to improve frozen soil, and from the microscopic view, the crack development, stress field, and the particle displacement field of the two samples were analyzed. The result shows that the force is not evenly distributed in the samples; with the main force chain on the cement particles, an increase in particles can lessen the cracks, and the failure of the 6% sample is a tensile plastic failure and that of the 18% sample is a tensile shear failure.
Influence of Blade Angle Deviation on the Hydraulic Performance and Structural Characteristics of S-Type Front Shaft Extension Tubular Pump Device
Authors: Shi Lijian , Wu Changxin , Wang Li , Xu Tian , Jiang Yuhang , Chai Yao , Zhu Jun
Published: February 2022

When the axial-flow pump is running, the blade angle is not fully adjusted or there are errors in the manufacture of the blades, which will lead to inconsistent blade placement angles during operation, and which will reduce the efficiency of the axial-flow pump. This paper uses the research methods of numerical simulation and model experiments to analyze the hydraulic performance and impeller structure characteristics of each flow components under different schemes when the angles of each blade of the S-type front shaft extension tubular pump device are inconsistent. The research phenomenon is that the guide vane greatly recovers the flow velocity circulation at the impeller outlet, reduces the hydraulic loss of guide vane, and widens the best efficiency range with an increase in guide vane blade angle. When the blade angle deviation occurs, the flow field of each blade channel affects each other, and the maximum decrease in the best efficiency is up to 7.78%, mainly due to the increased hydraulic loss in the outlet channel. The blade angle deviation will also affect the maximum equivalent stress and maximum deformation of the impeller, which is more obvious in large flow conditions. Inconsistent blade angles seriously affect the operating efficiency of the water pump and water pump device, and make the structural characteristics of the impeller worse.
Production of Non-Volatile Metabolites from Sooty Molds and Their Bio-Functionalities
Authors: Haituk Sukanya , Withee Patchareeya , Sangta Jiraporn , Senwanna Chanokned , Khamsaw Pattarapol , Karunarathna Anuruddha , Hongsibsong Surat , Sringarm Korawan , Prasad Shashanka K. , Sommano Sarana Rose , Cheewangkoon Ratchadawan
Published: February 2022

In the current study, eleven sooty mold isolates were collected from different tropical host plants. The isolates were identified under Capnodium, Leptoxyphium and Trichomerium, based on morphology and phylogeny. For the secondary metabolite analysis, the isolates were grown on Potato Dextrose Broth (PDB). The well-grown mycelia were filtered and extracted over methanol (MeOH). The metabolites in the growth medium (or filtrate) were extracted over ethyl acetate (EtOAc). The antifungal activities of each crude extract were tested over Alternaria sp., Colletotrichum sp., Curvularia sp., Fusarium sp. and Pestalotiopsis sp. The metabolites were further tested for their total phenolic, flavonoid and protein content prior to their antioxidant and anti-fungal potential evaluation. The MeOH extracts of sooty molds were enriched with proteins and specifically inhibited Curvularia sp. The total phenolic content and 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) activity was largely recovered from the filtrate corresponding to the inhibition of Alternaria sp.; while the flavonoid and free radical reduction suggested a relative induction of growth of the Fusarium sp., Colletotrichum sp. and Pestalotiopsis sp. Hence, this study reveals the diversity of sooty molds in Thailand by a modern phylogenetic approach. Furthermore, the preliminary screening of the isolates reveals the potential of finding novel compounds and providing insights for the future research on secondary metabolites of bio-trophic fungi and their potential usage on sustainable agriculture.
Real-World Testing of the Self Grasping Hand, a Novel Adjustable Passive Prosthesis: A Single Group Pilot Study
Authors: O’Brien Lisa , Montesano Elena , Chadwell Alix , Kenney Laurence , Smit Gerwin
Published: February 2022

(1) Background: This study investigated the feasibility of conducting a two-week “real-world” trial of the Self Grasping Hand (SGH), a novel 3D printed passive adjustable prosthesis for hand absence; (2) Methods: Single-group pilot study of nine adults with trans-radial limb absence; five used body-powered split-hooks, and four had passive cosmetic hands as their usual prosthesis. Data from activity monitors were used to measure wear time and bilateral activity. At the end of the two-week trial, function and satisfaction were measured using the Orthotics and Prosthetics Users’ Survey Function Scale (OPUS) and the prosthesis satisfaction sub-scales of the Trinity Amputations and Prosthesis Experience Scale (TAPES). Semi-structured interviews captured consumer feedback and suggestions for improvement; (3) Results: Average SGH wear time over 2 weeks was 17.5 h (10% of total prosthesis wear time) for split-hook users and 83.5 h (63% of total prosthesis wear time) for cosmetic hand users. Mean satisfaction was 5.2/10, and mean function score was 47.9/100; (4) Two-week real-world consumer testing of the SGH is feasible using the methods described. Future SGH designs need to be more robust with easier grasp lock/unlock.
Radiometric Assessment of ICESat-2 over Vegetated Surfaces
Authors: Neuenschwander Amy , Magruder Lori , Guenther Eric , Hancock Steven , Purslow Matt
Published: February 2022

The ice, cloud, and land elevation satellite-2 (ICESat-2) is providing global elevation measurements to the science community. ICESat-2 measures the height of the Earth’s surface using a photon counting laser altimeter, ATLAS (advanced topographic laser altimetry system). As a photon counting system, the number of reflected photons per shot, or radiometry, is a function primarily of the transmitted laser energy, solar elevation, surface reflectance, and atmospheric scattering and attenuation. In this paper, we explore the relationship between detected scattering and attenuation in the atmosphere against the observed radiometry for three general forest types, as well as the radiometry as a function of day versus night. Through this analysis, we found that ATLAS strong beam radiometry exceeds the pre-launch design cases for boreal and tropical forests but underestimates the predicted radiometry over temperate forests by approximately half a photon. The weak beams, in contrast, exceed all pre-launch conditions by a factor of two to six over all forest types. We also observe that the signal radiometry from day acquisitions is lower than night acquisitions by 10% and 40% for the strong and weak beams, respectively. This research also found that the detection ratio between each beam-pair was lower than the predicted 4:1 values. This research also presents the concept of ICESat-2 radiometric profiles; these profiles provide a path for calculating vegetation structure. The results from this study are intended to be informative and perhaps serve as a benchmark for filtering or analysis of the ATL08 data products over vegetated surfaces.
DGS-SLAM: A Fast and Robust RGBD SLAM in Dynamic Environments Combined by Geometric and Semantic Information
Authors: Yan Li , Hu Xiao , Zhao Leyang , Chen Yu , Wei Pengcheng , Xie Hong
Published: February 2022

Visual Simultaneous Localization and Mapping (VSLAM) is a prerequisite for robots to accomplish fully autonomous movement and exploration in unknown environments. At present, many impressive VSLAM systems have emerged, but most of them rely on the static world assumption, which limits their application in real dynamic scenarios. To improve the robustness and efficiency of the system in dynamic environments, this paper proposes a dynamic RGBD SLAM based on a combination of geometric and semantic information (DGS-SLAM). First, a dynamic object detection module based on the multinomial residual model is proposed, which executes the motion segmentation of the scene by combining the motion residual information of adjacent frames and the potential motion information of the semantic segmentation module. Second, a camera pose tracking strategy using feature point classification results is designed to achieve robust system tracking. Finally, according to the results of dynamic segmentation and camera tracking, a semantic segmentation module based on a semantic frame selection strategy is designed for extracting potential moving targets in the scene. Extensive evaluation in public TUM and Bonn datasets demonstrates that DGS-SLAM has higher robustness and speed than state-of-the-art dynamic RGB-D SLAM systems in dynamic scenes.
Concentration of Microparticles Using Flexural Acoustic Wave in Sessile Droplets
Authors: Peng Tao , Li Luming , Zhou Mingyong , Jiang Fengze
Published: February 2022

Acoustic manipulation of microparticles and cells has attracted growing interest in biomedical applications. In particular, the use of acoustic waves to concentrate particles plays an important role in enhancing the detection process by biosensors. Here, we demonstrated microparticle concentration within sessile droplets placed on the hydrophobic surface using the flexural wave. The design benefits from streaming flow induced by the Lamb wave propagated in the glass waveguide to manipulate particles in the droplets. Microparticles will be concentrated at the central area of the droplet adhesion plane based on the balance among the streaming drag force, gravity, and buoyancy at the operating frequency. We experimentally demonstrated the concentration of particles of various sizes and tumor cells. Using numerical simulation, we predicted the acoustic pressure and streaming flow pattern within the droplet and characterized the underlying physical mechanisms for particle motion. The design is more suitable for micron-sized particle preparation, and it can be valuable for various biological, chemical, and medical applications.