bluuebunny/crossref_metadata_2025 huggingface dataset:
Teaching Socio-Emotional Competencies Among Primary School Students: Improving Conflict Resolution and Promoting Democratic Co-existence in Schools
Authors: Santamaría-Villar María B. , Gilar-Corbi Raquel , Pozo-Rico Teresa , Castejón Juan L.
Published: June 2021

Teaching socio-emotional skills among primary school students is the key to creating a climate of cooperation in classrooms and reducing disruptive or aggressive behaviors among students. The primary goal of this research is to present an educational proposal for imparting socio-emotional competencies among primary school students. We attempt to impart socio-emotional competencies based on: (1) fostering self-knowledge, self-esteem, and respect for others among students; (2) developing behaviors that allow them to perceive and express feelings and self-regulating emotions; and (3) developing assertive communication skills aimed at improving conflict resolution. This program has been designed in such a way that it is implemented throughout the academic year by organizing bi-monthly sessions of 45 min each, held until the completion of 15 sessions. The sample consists of 100 students in the third grade, with the control and experimental groups having an equal number of students (50 each). The instruments used for this research are: (a) BarOn Emotional Quotient Inventory (Youth Version [BarOn EQ-i:YV]): used for measuring emotional and social functioning; (b) the Matson Evaluation of Social Skills with Youngsters (MESSY): used for assessing social skills; and (c) Questionnaire for the Assessment of School Violence in Preschool and Primary School Questionnaire. To check the effectiveness of the educational intervention, a quasi-experimental design, along with pretest-posttest control group design, is used in accordance with the general linear model. Its effectiveness is also checked using repeated measures analysis of variance. The results show that the program is useful in preventing violent behaviors in the educational field and promoting the development of socio-emotional skills among third grade students. Finally, the applicability of the program to other educational contexts is discussed to enhance students' personal development and decrease the levels of violence found in primary school.
Indigenous Midwives and the Biomedical System among the Karamojong of Uganda: Introducing the Partnership Paradigm
Authors: Graham Sally , Davis-Floyd Robbie
Published: June 2021

Certainly there can be no argument against every woman being attended at birth by a skilled birth attendant. Currently, as elsewhere, the Ugandan government favors a biomedical model of care to achieve this aim, even though the logistical realities in certain regions mitigate against its realisation. This article addresses the Indigenous midwives of the Karamojong tribe in Northeastern Uganda and their biosocial model of birth, and describes the need British midwife Sally Graham, who lived and worked with the Karamojong for many years, identified to facilitate “mutual accommodation” between biomedical staff and these midwives, who previously were reluctant to refer women to the hospital that serves their catchment area due to maltreatment by the biomedical practitioners there. This polarisation of service does not meet that society’s needs. We do not argue for the provision of a unilateral, top-down educational service, but rather for one that collaborates between the biosocial model of the Karamojong and the biomedical model supported by government legislation. We show that such a partnership is practical, safer, and harnesses the best and most economical and effective use of resources. In this article, we demonstrate the roles of the Indigenous midwives/traditional birth attendants (TBAs) and show that not only is marriage of the two systems both possible and desirable, but is also essential for meeting the needs of Karamojong women. The TBA is frequently all the skilled assistance available to these women, particularly during the rainy season when roads are impassable in rural South Karamoja. Without this skilled help, the incidence of maternal and infant mortality would undoubtedly increase. Ongoing training and supervision of the TBA/Indigenous midwife in best practices will ensure better care. We offer a way forward via the Partnership Paradigm (PP) that lead author Sally Graham designed in conjunction with the Indigenous midwives and biomedical staff with whom she worked, the development and characteristics of which this article describes.
Determinants of Sports Injury in Young Female Swedish Competitive Figure Skaters
Authors: Jederström Moa , Agnafors Sara , Ekegren Christina , Fagher Kristina , Gauffin Håkan , Korhonen Laura , Park Jennifer , Spreco Armin , Timpka Toomas
Published: June 2021

Introduction: Although figure skating attracts several hundred thousand participants worldwide, there is little knowledge about physical health and sports injuries among young skaters. The present study aimed to describe the health status of a geographically defined Swedish population of licensed competitive figure skaters and to examine injury determinants.

Methods: All licensed competitive skaters in the southeastern region of Sweden were in April 2019 invited to participate in a cross-sectional study using an online questionnaire. Multiple binary logistic regression was used for the examination of injury determinants. The primary outcome measure was the 1-year prevalence of a severe sports injury episode (time loss >21 days). The secondary outcome measure was the point prevalence of an ongoing injury. The determinants analyzed were age, skating level, relative energy deficiency indicators, and training habits.

Results: In total, 142 (36%) skaters participated, 137 (96%) girls [mean (SD) age: 12.9 (SD 3.0) years]. Participating boys (n = 5) were excluded from further analysis. The 1-year prevalence of a severe sports injury episode was 31%. The most common injury locations for these injuries were the knee (25%), ankle (20%), and hip/groin (15%). In the multiple model, having sustained a severe injury episode was associated with older age (OR 1.2, 95% CI 1.1–1.4; p = 0.002) and an increased number of skipped meals per week (OR 1.1, 95% CI 1.0–1.3; p = 0.014). The point prevalence of an ongoing injury episode was 19%. The most common locations were the knee (24%), ankle (24%), and foot (24%). Having an ongoing injury episode was associated with older age (OR 1.4, 95% CI 1.2–1.7; p < 0.001) and an increased number of skipped meals per week (OR 1.1, 95% CI 1.0–1.3; p = 0.049).

Conclusion: One-third of young female Swedish competitive figure skaters had sustained a severe injury episode during the past year, and a fifth reported an ongoing episode. Older age and an increased number of skipped meals per week were associated with a sports injury episode. Long-term monotonous physical loads with increasing intensity and insufficient energy intake appear to predispose for injury in young female figure skaters. Further examination of injury determinants among competitive figure skaters is highly warranted.
Students' Knowledge of Healthy Food and Their Actual Eating Habits: A Case Study on the University of Granada (Spain)
Authors: Entrena-Durán Francisco , Baldan-Lozano Henar , Valdera-Gil Juan-Miguel
Published: June 2021

This article focuses on an analysis of the discourses produced during 34 semi-structured interviews (17 men and 17 women) conducted at the University of Granada (Spain) with undergraduate, Master, and Ph.D. students. The interviewees were between 20 and 44 years old. It was observed that the fact of having a high educational level did not prevent University students from eating unhealthily. There is a gap between the fact that 97.1% of 34 students interviewed (that is, 33 of them) know what healthy food is and their self-perception about whether or not what they are eating is healthy, since in 41.2% of them said self-perception is negative. This gap narrows as the interviewees' age increases and their socio-economic and vital situation is stabilizing which favors that their eating habits become more regular and healthier. Thus, all the interviewees aged 27 or over self-perceived that they were eating healthily. But the biggest differences are those that have to do with the gender of interviewees. Thus, while 23.5% of women interviewed perceived that they were not eating healthy, 76.5% of them felt that they were eating healthy. However, among the men interviewed, these percentages were somehow reversed, in such a way that 58.8% of them believed that they were not eating healthy, compared to 41.2% of them who indicated that they were eating healthy. Therefore, the investigation revealed that women tend to have the best chances of assuming healthy eating habits. Male students living outside the family home or without female partners exhibited greater feeding problems, while females living under similar conditions tended to display healthier eating habits. This is related to the fact that women have traditionally been in charge of acquiring and preparing food. So, women's food education has not been restricted to the mere transmission to them of knowledge about what healthy food is, but from their childhood they were food trained through their active involvement in practical experiences. Obviously, the solution proposed to this male disadvantage is to not perpetuate macho gender stereotypes that assign women the role of home caregivers, but to seek that both women and men have the opportunity and the duty to experience equally those practical experiences that involve the tasks of the acquisition and preparation of food. Working to achieve a situation like this, not only promotes progress in gender equality, but also helps to overcome the lower training of men to perform the tasks inherent in their diet.
Lateral Stability Control of Four-Wheel-Drive Electric Vehicle Based on Coordinated Control of Torque Distribution and ESP Differential Braking
Authors: Chen Liqing , Li Zhiqiang , Yang Juanjuan , Song Yu
Published: June 2021

This research focuses on four-wheel-drive electric vehicles. On the basis of the hierarchical coordinated control strategy, the coordinated control system of driving force distribution regulation and differential braking regulation was designed to increase the electric vehicles steering stability under special road working conditions. A seven-degree-of-freedom model of an electric vehicle was established in MATLAB/Simulink, and then a hierarchical coordination control model of the Electronic stability program and dynamic torque distribution control system was established. Adaptive fuzzy control was applied to ESP and, based on the neural network PID control, a torque distribution control system was designed. On the basis of the proposed coordinated control model, a performance simulation and a hardware-in-the-loop test of the control system under the typical working condition of single line shift were carried out. From the final results, it can be seen that the proposed control strategy can greatly improve the safety of the vehicle after serious side slip, increase the stability of the whole vehicle, and effectively increase the vehicle lateral stability.
A Biostimulant Based on Seaweed (Ascophyllum nodosum and Laminaria digitata) and Yeast Extracts Mitigates Water Stress Effects on Tomato (Solanum lycopersicum L.)
Authors: Campobenedetto Cristina , Agliassa Chiara , Mannino Giuseppe , Vigliante Ivano , Contartese Valeria , Secchi Francesca , Bertea Cinzia M.
Published: June 2021

Water deficit is one of the most problematic stressors worldwide. In this context, the use of biostimulants represents an increasingly ecological practice aimed to improve crop tolerance and mitigate the negative effects on the productivity. Here, the effect derived from the foliar application of ERANTHIS®®, a biostimulant based on seaweed (Ascophyllum nodosum and Laminaria digitata) and yeast extracts, was tested on tomato plants grown under mild water-stress conditions. The potential stress mitigation action was evaluated by monitoring morphometric (fresh weight and dry matter content), physiological (stem water potential) and biochemical (ROS scavenger enzymes activity, proline, abscisic acid, hydrogen peroxide and photosynthetic pigment content) parameters closely related to the occurrence and response to stress at both flowering and fruit-set timing. In general, we observed that plants grown under drought conditions and treated with the biostimulant had a lower amount of ABA, and MDA and proline correlated to a lower activity of ROS scavenger enzymes compared to untreated plants. These data, together with the higher stem water potential and photosynthetic pigment levels recorded for the treated plants, suggest that ERANTHIS®® may mitigate water stress effects on tomato.
First Report on the Mechanisms of Insecticide Resistance in Field Populations of the Small Hive Beetle in Florida
Authors: Kanga Lambert H. B. , Marechal Walker , Ananga Anthony
Published: June 2021

The small hive beetle (Aethina tumida Murray) is a serious threat to beekeeping and crops that rely on honeybees for pollination. The small hive beetle not only causes significant damage to honeybees by feeding on pollen and honey, attacking bee brood and causing stored honey to ferment, but also might serve as a vector of diseases. In addition, the small hive beetle has developed resistance to the pyrethroid and organophosphate insecticides registered for control of honeybee pests in the United States. The development of resistance in small hive beetle populations is a great concern to the beekeeping industry; thus, there is an urgent need for strategies to manage that resistance. Therefore, we used synergist probes to determine the mechanisms of resistance in a small hive beetle population to these insecticides. Our studies on the toxicity of insecticides alone or with the synergists piperonyl butoxide (PBO) and S,S,S,-tributyl phosphorotrithionate (DEF) suggested that mixed-function oxidases and esterases were the major resistance factors to these insecticides in a studied population of the small hive beetle. In contrast, there was no synergism with diethyl maleate (DEM), triphenyl phosphate (TPP) and formamidine. Therefore, glutathione-S-transferase, carboxylesterase and target site were not involved in insecticide resistance in the small hive beetle. Rotation of classes of insecticides (with different modes of action) and metabolic synergists were suggested for the development of successful resistance management programs. To the best of our knowledge, this is the first study of the mechanisms of resistance in small hive beetle populations in Florida and suggests an urgent need for alternative control strategies for these serious pests of honeybee colonies.
The Importance of Environmental Factors for the Development of Water Erosion of Soil in Agricultural Land: The Southern Part of Hronská Pahorkatina Hill Land, Slovakia
Authors: Petlušová Viera , Petluš Peter , Ševčík Michal , Hreško Juraj
Published: June 2021

The water erosion research was carried out in the lowland type of hilly landscape. The aim was to monitor and evaluate the importance of environmental factors (steepness of slope, relief shapes, aspect, slope length, combination slope length (L) and slope (S)—LS factor, types of land use changes) for the development of water erosion. We focused on the identification of areas threatened by erosion by interpreting aerial photographs from several time periods. This was followed by verification of erosion using soil probes. We identified 408.44 ha of areas affected by erosion, and measured the depth of soil and “A” horizons thickness. The environmental factors were modeled in geographical information systems by tools for spatially oriented data. Subsequently, the influence and significance of individual environmental factors were compared, and the probability of erosion was statistically estimated. The decisive factors in the formation of erosive surfaces are the LS factor and the slope. We also consider the factor of the relief shape to be important. The shape did not appear to be very significant as a separately evaluated factor, but all convex parts correlate with the identified erosion surfaces. The susceptibility of erosion related to the aspect of the slopes to the cardinal directions has not been confirmed. Types of land use changes with the most significant relation of erosion were confirmed in areas of strong intensification. We confirmed the importance of factors and land use for the development of erosion processes.
How Different Personalities Affect the Reaction to Adoption of Dogs Adopted from a Shelter
Authors: Corsetti Sara , Pimpolari Luisa , Natoli Eugenia
Published: June 2021

Dog shelters provide a valuable service by housing homeless dogs and seeking subsequent adoption for these dogs. Few studies have aimed to monitor the behavior of adopted dogs when adoption is successful. The aim of this study was to detect what behavioral modifications, based on their personality, occurred in dogs after their adoption. The personality of 34 healthy dogs was evaluated in the pre-adoption phase by means of a Principal Component Analysis (PCA) of their behavioral patterns. In the post-adoption phase, we analyzed the behavior of the same dogs, completing a questionnaire with their owners. Pre- and post-adoption data were standardized and a PCA was run on the differences between these variables. A k-means cluster analysis was run on the six components, obtaining three groups of dogs: for groups one and two, changes in behavior after adoption seemed to be influenced by dog personality: bolder dogs (1st group) became more active, excitable and playful, showed increased aggressive behavior towards humans, and decreased anxious and submissive behavior towards dogs and humans; shyer dogs (2nd group) went in the opposite direction, displaying increased aggressive behavior. For the 3rd group, personality was not predictive of behavior changes. All the dog adoptions in this study were successful.
Alternative and Unconventional Feeds in Dairy Diets and Their Effect on Fatty Acid Profile and Health Properties of Milk Fat
Authors: Hadrová Sylvie , Sedláková Kateřina , Křížová Ludmila , Malyugina Svetlana
Published: June 2021

Milk fat is an important nutritional compound in the human diet. From the health point of view, some fatty acids (FAs), particularly long-chain PUFAs such as EPA and DHA, have been at the forefront of interest due to their antibacterial, antiviral, anti-inflammatory, and anti-tumor properties, which play a positive role in the prevention of cardiovascular diseases (CVD), as well as linoleic and γ-linolenic acids, which play an important role in CVD treatment as essential components of phospholipids in the mitochondria of cell membranes. Thus, the modification of the FA profile—especially an increase in the concentration of polyunsaturated FAs and n-3 FAs in bovine milk fat—is desirable. The most effective way to achieve this goal is via dietary manipulations. The effects of various strategies in dairy nutrition have been thoroughly investigated; however, there are some alternative or unconventional feedstuffs that are often used for purposes other than basic feeding or modifying the fatty acid profiles of milk, such as tanniferous plants, herbs and spices, and algae. The use of these foods in dairy diets and their effects on milk fatty acid profile are reviewed in this article. The contents of selected individual FAs (atherogenic, rumenic, linoleic, α-linolenic, eicosapentaenoic, and docosahexaenoic acids) and their combinations; the contents of n3 and n6 FAs; n6/n3 ratios; and atherogenic, health-promoting and S/P indices were used as criteria for assessing the effect of these feeds on the health properties of milk fat.
Veterinary Education during Covid-19 and Beyond—Challenges and Mitigating Approaches
Authors: Routh Jennifer , Paramasivam Sharmini Julita , Cockcroft Peter , Nadarajah Vishna Devi , Jeevaratnam Kamalan
Published: June 2021

The public health implications of the Covid-19 pandemic have caused unprecedented and unexpected challenges for veterinary schools worldwide. They are grappling with a wide range of issues to ensure that students can be trained and assessed appropriately, despite the international, national, and local restrictions placed on them. Moving the delivery of knowledge content largely online will have had a positive and/or negative impact on veterinary student learning gain which is yet to be clarified. Workplace learning is particularly problematic in the current climate, which is concerning for graduates who need to develop, and then demonstrate, practical core competences. Means to optimise the learning outcomes in a hybrid model of curriculum delivery are suggested. Specific approaches could include the use of video, group discussion, simulation and role play, peer to peer and interprofessional education.
Influence of Residual Feed Intake and Cow Age on Dry Matter Intake Post-Weaning and Peak Lactation of Black Angus Cows
Authors: Parsons Cory T. , Dafoe Julia M. , Wyffels Samuel A. , DelCurto Timothy , Boss Darrin L.
Published: June 2021

We evaluated heifer post-weaning residual feed intake (RFI) classification and cow age on dry matter intake (DMI) at two stages of production. Fifty-nine non-lactating, pregnant, (Study 1) and fifty-four lactating, non-pregnant (Study 2) commercial black Angus beef cows were grouped by age and RFI. Free-choice, hay pellets were fed in a GrowSafe feeding system. In Study 1, cow DMI (kg/d) and intake rate (g/min) displayed a cow age effect (p < 0.01) with an increase in DMI and intake rate with increasing cow age. In Study 2, cow DMI (kg/d) and intake rate (g/min) displayed a cow age effect (p < 0.02) with an increase in DMI and intake rate with increasing cow age. Milk production displayed a cow age × RFI interaction (p < 0.01) where both 5–6-year-old and 8–9-year-old low RFI cows produced more milk than high RFI cows. For both studies, intake and intake behavior were not influenced by RFI (p ≥ 0.16) or cow age × RFI interaction (p ≥ 0.21). In summary, heifer’s post-weaning RFI had minimal effects on beef cattle DMI or intake behavior, however, some differences were observed in milk production.
A Machine Learning Approach to Study Demographic Alterations in Honeybee Colonies Using SDS–PAGE Fingerprinting
Authors: Cabbri Riccardo , Ferlizza Enea , Bellei Elisa , Andreani Giulia , Galuppi Roberta , Isani Gloria
Published: June 2021

Honeybees, as social insects, live in highly organised colonies where tasks reflect the age of individuals. As is widely known, in this context, emergent properties arise from interactions between them. The accelerated maturation of nurses into foragers, stimulated by many negative factors, may disrupt this complex equilibrium. This complexity needs a paradigm shift: from the study of a single stressor to the study of the effects exerted by multiple stressors on colony homeostasis. The aim of this research is, therefore, to study colony population disturbances by discriminating overaged nurses from proper aged nurses and precocious foragers from proper aged foragers using SDS-PAGE patterns of haemolymph proteins and a machine-learning algorithm. The KNN (K Nearest Neighbours) model fitted on the forager dataset showed remarkably good performances (accuracy 0.93, sensitivity 0.88, specificity 1.00) in discriminating precocious foragers from proper aged ones. The main strength of this innovative approach lies in the possibility of it being deployed as a preventive tool. Depopulation is an elusive syndrome in bee pathology and early detection with the method described could shed more light on the phenomenon. In addition, it enables countermeasures to revert this vicious circle.
Analysis of the Gastro-Intestinal Tract of Marine Mammals: A Multidisciplinary Approach with a New Multi-Sieves Tool
Authors: Corazzola Giorgia , Baini Matteo , Grattarola Carla , Panti Cristina , Marcer Federica , Garibaldi Fulvio , Berio Enrica , Mancusi Cecilia , Galli Matteo , Mazzariol Sandro , Fossi Maria Cristina , Centelleghe Cinzia , Casalone Cristina
Published: June 2021

Organs and content of the gastro-intestinal tract (GIT) of marine mammals are relevant for a variety of investigations and provide data to researchers from different fields. Currently used protocols applied to the GIT for specific analysis limit the possibility to execute other investigations and important information could be lost. To ensure a proper sample collection and a multidisciplinary investigation of the GIT of marine mammals, a new multi-sieves tool and a specific protocol have been developed. This new device and approach allowed the simultaneous sampling of the GIT and its content for the main investigations concerned. The samples collected during these preliminary trials were suitable to perform all the different research procedures considered in this work. The obtained results show that with a few and easy procedural adjustments, a multidisciplinary sampling and evaluation of the GIT of marine mammals is possible. This will reduce the risk of losing important data aimed at understanding the cause of death of the animal, but also biology and ecology of marine mammals, and other important data for their conservation and habitats management.
Metagenomic Analysis of the Fecal Archaeome in Suckling Piglets Following Perinatal Tulathromycin Metaphylaxis
Authors: Zeineldin Mohamed , Megahed Ameer , Blair Benjamin , Aldridge Brian , Lowe James
Published: June 2021

The gastrointestinal microbiome plays an important role in swine health and wellbeing, but the gut archaeome structure and function in swine remain largely unexplored. To date, no metagenomics-based analysis has been done to assess the impact of an early life antimicrobials intervention on the gut archaeome. The aim of this study was to investigate the effects of perinatal tulathromycin (TUL) administration on the fecal archaeome composition and diversity in suckling piglets using metagenomic sequencing analysis. Sixteen litters were administered one of two treatments (TUL; 2.5 mg/kg IM and control (CONT); saline 1cc IM) soon after birth. Deep fecal swabs were collected from all piglets on days 0 (prior to treatment), 5, and 20 post intervention. Each piglet’s fecal archaeome was composed of rich and diverse communities that showed significant changes over time during the suckling period. At the phylum level, 98.24% of the fecal archaeome across all samples belonged to Euryarchaeota. At the genus level, the predominant archaeal genera across all samples were Methanobrevibacter (43.31%), Methanosarcina (10.84%), Methanococcus (6.51%), and Methanocorpusculum (6.01%). The composition and diversity of the fecal archaeome between the TUL and CONT groups at the same time points were statistically insignificant. Our findings indicate that perinatal TUL metaphylaxis seems to have a minimal effect on the gut archaeome composition and diversity in sucking piglets. This study improves our current understanding of the fecal archaeome structure in sucking piglets and provides a rationale for future studies to decipher its role in and impact on host robustness during this critical phase of production.
Objective Assessment of Chronic Pain in Horses Using the Horse Chronic Pain Scale (HCPS): A Scale-Construction Study
Authors: van Loon Johannes P. A. M. , Macri Lucia
Published: June 2021

The objective assessment of chronic pain is of utmost importance for improving welfare and quality of life in horses. Freedom from disease and pain is one of the ‘five freedoms’ that are necessary for animal welfare. The aim of this study was to develop a pain scale for the assessment of chronic pain in horses (Horse Chronic Pain Scale; HCPS), which is based on behavioural and facial expressions. The scale was used to assess 53 horses (26 horses diagnosed with chronic painful conditions by means of clinical examination and additional diagnostic procedures (consisting of osteoarthritis, chronic laminitis, chronic back and neck problems, chronic dental disorders) and 27 healthy control animals). Animals were assessed once daily for three consecutive days by two observers that were blinded to the condition of the animals and were unaware of any analgesic treatment regimens. The HCPS consists of two parts, the Horse Chronic Pain Composite Pain Scale (HCP CPS, with behavioural parameters) and the EQUUS-FAP (Equine Utrecht University Scale for Facial Assessment of Pain). The HCP CPS had good inter-observer reliability (intraclass correlation coefficient (ICC) = 0.84, p < 0.001), while the EQUUS-FAP component (with facial expression-based parameters) had poor inter-observer reliability (ICC = 0.45, p < 0.05). The inter-observer reliability of the combined HCPS was good (ICC = 0.78, p < 0.001). The HCPS revealed significant differences between horses with chronic painful conditions and control horses on 2 out of 3 days (p < 0.05). In conclusion, we tested a composite pain scale for the assessment of chronic pain in horses based on behavioural and facial expression-based parameters. Further studies are needed to validate this pain scale before it can be used in practice.
Sperm Behavior and Response to Melatonin under Capacitating Conditions in Three Sheep Breeds Subject to the Equatorial Photoperiod
Authors: Carvajal-Serna Melissa , Cardozo-Cerquera Jaime Antonio , Grajales-Lombana Henry Alberto , Casao Adriana , Pérez-Pe Rosaura
Published: June 2021

In this study, we demonstrated that, in seasonal Mediterranean ovine breeds, supplementing the TALP medium with cAMP-elevating agents (the cocktail medium) is effective for achieving ram sperm capacitation, and that melatonin is able to regulate this phenomenon. We investigated the behavior under capacitating conditions using the TALP and cocktail mediums, and the response to melatonin, of spermatozoa from three sheep breeds (Colombian Creole, Romney Marsh, and Hampshire) subject to the equatorial photoperiod, during the dry and the rainy seasons. The cocktail medium was able to induce sperm capacitation, assayed by chlortetracycline staining and phosphotyrosine levels, to a greater extent than TALP, without a higher loss of viability (membrane integrity and viable spermatozoa without phosphatidylserine (PS) translocation). The addition of melatonin at 100 pM or 1 µM in the cocktail medium partially prevented the decrease in viability without PS translocation and the increase in capacitated spermatozoa from all breeds, with no significant effect on phosphotyrosine levels. Differences between breeds and seasons were evidenced. This study shows that melatonin is able to exert direct effects on spermatozoa in ovine breeds under equatorial photoperiod conditions, as it does in seasonal breeds located in temperate regions.
New Insights on NETosis Induced by Entamoeba histolytica: Dependence on ROS from Amoebas and Extracellular MPO Activity
Authors: Díaz-Godínez César , Jorge-Rosas Joshue Fabián , Néquiz Mario , Martínez-Calvillo Santiago , Laclette Juan P. , Rosales Carlos , Carrero Julio C.
Published: June 2021

NETosis is a neutrophil process involving sequential steps from pathogen detection to the release of DNA harboring antimicrobial proteins, including the central generation of NADPH oxidase dependent or independent ROS. Previously, we reported that NETosis triggered by Entamoeba histolytica trophozoites is independent of NADPH oxidase activity in neutrophils, but dependent on the viability of the parasites and no ROS source was identified. Here, we explored the possibility that E. histolytica trophozoites serve as the ROS source for NETosis. NET quantitation was performed using SYTOX® Green assay in the presence of selective inhibitors and scavengers. We observed that respiratory burst in neutrophils was inhibited by trophozoites in a dose dependent manner. Mitochondrial ROS was not also necessary, as the mitochondrial scavenger mitoTEMPO did not affect the process. Surprisingly, ROS-deficient amoebas obtained by pre-treatment with pyrocatechol were less likely to induce NETs. Additionally, we detected the presence of MPO on the cell surface of trophozoites after the interaction with neutrophils and found that luminol and isoluminol, intracellular and extracellular scavengers for MPO derived ROS reduced the amount of NET triggered by amoebas. These data suggest that ROS generated by trophozoites and processed by the extracellular MPO during the contact with neutrophils are required for E. histolytica induced NETosis.
Cytoprotective Effects of Fish Protein Hydrolysates against H2O2-Induced Oxidative Stress and Mycotoxins in Caco-2/TC7 Cells
Authors: Taroncher Mercedes , Rodríguez-Carrasco Yelko , Aspevik Tone , Kousoulaki Katerina , Barba Francisco J. , Ruiz María-José
Published: June 2021

Many studies report the potent antioxidant capacity for fish protein hydrolysates, including radical scavenging activity and inhibition ability on lipid peroxidation (LPO). In this study, the in vitro cytotoxicity of protein hydrolysates from different salmon, mackerel, and herring side streams fractions was evaluated in the concentration range from 1 to 1:32 dilution, using cloned human colon adenocarcinoma cells TC7 (Caco-2/TC7) by MTT and PT assays. The protein hydrolysates’ antioxidant capacity and oxidative stress effects were evaluated by LPO and reactive oxygen species (ROS) generation, respectively. The antioxidant capacity for pure and bioavailable hydrolysate fraction was also evaluated and compared. Additionally, mycotoxin levels were determined in the fish protein hydrolysates, and their cytoprotective effect against T-2 toxin was evaluated. Both hydrolysates and their bioavailable fraction induced similar cell viability rates. The highest cytoprotective effect was obtained for the salmon viscera protein hydrolysate (HSV), which increased the cell viability by 51.2%. ROS accumulation induced by H2O2 and LPO was suppressed by all pure hydrolysates. The cytoprotective effect of hydrolysates was observed against T-2. Moreover, the different fish fraction protein hydrolysates contain variable nutrients and unique bioactive peptide composition showing variable bioactivity, which could be a useful tool in developing dietary supplements with different target functional properties.
Inhibition of Oxidative Stress and ALOX12 and NF-κB Pathways Contribute to the Protective Effect of Baicalein on Carbon Tetrachloride-Induced Acute Liver Injury
Authors: Dai Chongshan , Li Hui , Wang Yang , Tang Shusheng , Velkov Tony , Shen Jianzhong
Published: June 2021

This study investigates the protective effect of baicalein on carbon tetrachloride (CCl4)-induced acute liver injury and the underlying molecular mechanisms. Mice were orally administrated baicalein at 25 and 100 mg/kg/day for 7 consecutive days or ferrostatin-1 (Fer-1) at 10 mg/kg was i.p. injected in mice at 2 and 24 h prior to CCl4 injection or the vehicle. Our results showed that baicalein or Fer-1 supplementation significantly attenuated CCl4 exposure-induced elevations of serum alanine aminotransferase and aspartate aminotransferase, and malondialdehyde levels in the liver tissues and unregulated glutathione levels. Baicalein treatment inhibited the nuclear factor kappa-B (NF-κB) pathway, activated the erythroid 2-related factor 2 (Nrf2)/heme oxygenase 1 (HO-1) pathway in liver tissues, and markedly improved CCl4-induced apoptosis, inflammation and ferroptosis in liver tissues exposed with CCl4. In vitro, baicalein treatment improved CCl4 -induced decreases of cell viabilities and knockdown of Nrf2 and arachidonate 12-lipoxygenase (ALOX12) genes partly abolished the protective effect of baicalein on CCl4 -induced cytotoxicity in HepG2 cells. In conclusion, our results reveal that baicalein supplementation ameliorates CCl4-induced acute liver injury in mice by upregulating the antioxidant defense pathways and downregulating oxidative stress, apoptosis, inflammation and ferroptosis, which involved the activation of Nrf2 pathway and the inhibition of ALOX12 and NF-κB pathways.
Assessment of Fit on Ten Screw-Retained FrameworksRealized through Digital Full-Arch Implant Impression
Authors: De Francesco Maurizio , Stellini Edoardo , Granata Stefano , Mazzoleni Sergio , Ludovichetti Francesco Saverio , Monaco Carlo , Di Fiore Adolfo
Published: June 2021

Background: Discordant opinions have emerged among clinicians and researchers regarding a digital impression for full-arch implant-supported fixed dental prostheses (FDPs). The purpose of this study was to assess the fit of screw-retained milled frameworks on six implants realized from digital impressions through the Sheffield test. Methods: One patient received a maxillary full-arch implant-supported FDP. Six months after the surgical procedure, ten intraoral full-arch digital impressions were performed to mill ten frameworks. To clinically assess the fit, the Sheffield test was applied for all frameworks. The gaps among the frameworks and the implant analogs were measured using a microscope on the master model realized with a traditional impression. The Wilcoxon sum-rank test was used to compare the misfit value among the different implant positions. Results: The Sheffield test did not show gaps in the framework–implant interfaces when the screw was completely tightened on the more distal implant for all the milled frameworks. The mean misfit value calculated after microscope examination was 38 ± 5 μm. Differences that were statistically significant emerged when the misfit values of central positions were compared with other values. Conclusions: The use of full-arch implant digital impressions represents a viable alternative to traditional impressions for the fabrication of implant-supported FDPs.
Bioresorption Control and Biological Response of Magnesium Alloy AZ31 Coated with Poly-β-Hydroxybutyrate
Authors: Wang Lu , Aversa Raffaella , Houa Zhengjun , Tian Jie , Liang Shuang , Ge Shuping , Chen Yu , Perrotta Valeria , Apicella Antonio , Apicella Davide , Cioffi Luigi , Wang Guixue
Published: June 2021

Magnesium and its alloys are not normally used as bioresorbable temporary implants due to their high and uncontrolled degradation rate in a physiological liquid environment. The improvement of corrosion resistance to simulated body fluids (SBF) of a magnesium alloy (AZ31) coated with poly-β-hydroxybutyrate (PHB) was investigated. Scanning electron microscopy, Fourier transform infrared spectrometer, and contact angle measurements were used to characterize surface morphology, material composition, and wettability, respectively. pH modification of the SBF corroding medium, mass of Mg2+ ions released, weight loss of the samples exposed to the SBF solution, and electrochemical experiments were used to describe the corrosion process and its kinetics. The material’s biocompatibility was described by evaluating the effect of corrosion by products collected in the SBF equilibrating solution on hemolysis ratio, cytotoxicity, nitric oxide (NO), and total antioxidant capacity (T-AOC). The results showed that the PHB coating can diffusively control the degradation rate of magnesium alloy, improving its biocompatibility: the hemolysis rate of materials was lower than 5%, while in vitro human umbilical vein endothelial cell (HUVEC) compatibility experiments showed that PHB-coated Mg alloy promoted cell proliferation and had no effect on the NO content and that the T-AOC was enhanced compared with the normal group and bare AZ31 alloy. PHB-coated AZ31 magnesium alloy extraction fluids have a less toxic behavior due to the lower concentration of corrosion byproducts deriving from the diffusion control exerted by the PHB coating films both from the metal surface to the solution and vice versa. These findings provide more reference value for the selection of such systems as tunable bioresorbable prosthetic materials.
Reactive Powder Concrete: Durability and Applications
Authors: Sanjuán Miguel Ángel , Andrade Carmen
Published: June 2021

Reactive powder concrete (RPC) is an ultra-high-performance concrete (UHPC) developed years ago by Bouygues, with the aim to build strong, durable, and sustainable structures. Some differences can be underlined between the RPC and high-performance concrete (HPC); that is to say, RPC exhibits higher compressive and flexural strength, higher toughness, lower porosity, and lower permeability compared to HPC. Microstructural observations confirm that silica fume enhances the fiber–matrix interfacial characteristics, particularly in fiber pullout energy. This paper reviews the reported literature on RPC, and it offers a comparison between RPC and HPC. Therefore, some RPC potential applications may be inferred. For instance, some examples of footbridges and structural repair applications are given. Experimental measurements on air permeability, porosity, water absorption, carbonation rate, corrosion rate, and resistivity are evidence of the better performance of RPC over HPC. When these ultra-high-performance concretes are reinforced with discontinuous, short fibers, they exhibit better tensile strain-hardening performance.
Polyethylenimine-Modified Magnetic Chitosan for the Uptake of Arsenic from Water
Authors: Alsaiari Norah Salem , Alzahrani Fatimah Mohammed , Katubi Khadijah Mohammedsaleh , Amari Abdelfattah , Rebah Faouzi Ben , Tahoon Mohamed A.
Published: June 2021

The removal of heavy metals from water has become a global environmental problem. Various materials have been applied as adsorbent to remove metals from water. In this field, nanomaterials have been gaining increasing interest due to their exceptional properties. In this work, we discuss the synthesis of a core-shell structure nanocomposite by the modification of magnetic chitosan (CS) (Fe3O4/CS) with polyethylenimine (PEI) to produce Fe3O4/CS/PEI composite for the adsorption of arsenic ions (As(V) and As(III)) from aqueous solution. The synthesized materials were characterized using X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), and vibrating sample magnetometer (VSM). The results indicated the successful combination of three components of the nanocomposite. The adsorption conditions were optimized by studying the effect of different parameters included pH, contact time, initial concentration, and adsorbent dosage. The optimum adsorption pH was found to be 6.7 while the optimum adsorbent dosage was found to be 2.0 and 1.5 g/L for As(III) and As(V), respectively. The removal efficiency for the uptake of As(III) and As(V) ions over Fe3O4/CS/PEI nanocomposite at optimum conditions was found to be 99.5 and 99.7%, respectively. The experimental results were fitted using Freundlich’s and Langmuir’s isotherms. The data were more fitted to Langmuir isotherm providing a suggestion of monolayer adsorption with maximum adsorption capacity equal to 77.61 and 86.50 mg/g for the removal of As(III) and As(V), respectively. Moreover, linear regression coefficient (R2) indicated that the adsorption of arsenic ions over the synthesized magnetic nanocomposite obeyed pseudo 2nd order suggesting the chemisorption process. The reusability of the nanosorbent for arsenic uptake using sodium hydroxide as eluent was also assessed up to five cycles. Interestingly, Fe3O4/CS/PEI nanocomposite can be considered as a promising adsorbent for As ions’ removal from water and should be tested for the removal of other pollutants.
Semantic Microservice Framework for Digital Twins
Authors: Steindl Gernot , Kastner Wolfgang
Published: June 2021

Digital Twins (DT) in industrial cyber-physical systems are the key enabling technology for Industry 4.0. Services are an essential part of almost every DT concept, but their interaction is usually implementation-specific since no common guidelines are available. This work identifies some fundamental requirements for a DT service framework based on applications identified in corresponding literature. Based on these requirements, a service framework architecture is proposed. The architecture utilizes Semantic Web technology and a workflow engine for service orchestration to support the fulfilment of the identified requirements. As a case study for sensor data evaluation of an industrial process, a proof-of-concept implementation is presented, showing the feasibility and suitability of the proposed DT service framework architecture.
Interdisciplinary Learning Methodology for Supporting the Teaching of Industrial Radiology through Technical Drawing
Authors: Vergara Diego , Rubio Manuel P. , Extremera Jamil , Lorenzo Miguel
Published: June 2021

Technical drawing (TD) is a subject frequently perceived by engineering students as difficult and even lacking in practical application. Different studies have shown that there is a relationship between studying TD and improvement of spatial ability, and there are precedents of works describing successful educational methodologies based on information and communications technology (ICT), dedicated in some cases to improving spatial ability, and in other cases to facilitating the teaching of TD. Furthermore, interdisciplinary learning (IL) has proven to be effective for the training of science and engineering students. Based on these facts, this paper presents a novel IL educational methodology that, using ICT-based tools, links the teaching of industrial radiology with the teaching of TD, enhancing the spatial ability of students. First, the process of creating the didactic material is described in summary form, and thereafter, the way in which this educational methodology is implemented in the classroom. Finally, we analyze how the use of ICT-based didactic tools such as the one described in this paper can contribute to the achievement of the sustainable development goals set out in the 2030 Agenda of the United Nations.
Task-Based Design Approach: Development of a Planar Cable-Driven Parallel Robot for Upper Limb Rehabilitation
Authors: Ennaiem Ferdaws , Chaker Abdelbadiâ , Laribi Med Amine , Sandoval Juan , Bennour Sami , Mlika Abdelfattah , Romdhane Lotfi , Zeghloul Saïd
Published: June 2021

This paper deals with the optimal design of a planar cable-driven parallel robot (CDPR), with three degrees of freedom, intended for assisting the patient’s affected upper limb along a prescribed movement. A Qualisys motion capture system was used to record the prescribed task performed by a healthy subject. For each pose taken by the center of mass of the end-effector, the cable tensions, the elastic stiffness and the dexterity were optimized while satisfying a set of constraints. First, a multiobjective formulation of the optimization problem was adopted. Since selecting a single solution among the multiple ones given by the Pareto front presents an issue, a mono-objective formulation was chosen, where the objective function was defined as a weighted sum of the chosen criteria. The appropriate values of the weighted coefficients were studied with the aim of identifying their influence on the optimization process and, thus, a judicious choice was made. A prototype of the optimal design of the CDPR was developed and validated experimentally on the prescribed workspace using the position control approach for the motors. The tests showed promising reliability of the proposed design for the task.