bluuebunny/crossref_metadata_2025 huggingface dataset:
The value of completion residual lung resection in ipsilateral recurrent non-small cell lung cancer
Authors: Yang Yong , Wang Yichao , Wan Ziwei , Qin Xiong , Zhu Yuming , Sheng Bingyong , Zhao Xiaogang
Published: November 2022

Background

Recurrence is one of the most important challenges to manage lung cancer. Selected patients might be candidates for resection. This study assessed the outcomes and hazard factors of patients after completion of lung resection for recurrence, focusing specifically on postrecurrence survival (PRS) and overall survival (OS) after surgery.

Methods

This retrospective study enrolled 63 patients who underwent complete pulmonary resection for recurrence between January 2015 and December 2018. Inclusion criteria include potentially curative first resection for primary lung cancer, histologically proven recurrent or new malignancy, and complete pathological report after both operations. PRS and OS were assessed and the influence of patient and treatment features on these endpoints was evaluated.

Results

Most of the patients recurred at stage IIIA, and nearly three-fourth received complete pneumonectomy. The overall 2- and 5-year survival rates were 95% and 75%, whereas the overall 2- and 5-year postrecurrence survival rates were 55% and 36%, respectively. No patient died within 30 or 90 days after completion of residual lung resection, and no serious complications occurred during follow-up. Upon selection of clinically important variables by the Cox proportional hazards regression model, the r-stage [hazard ratio (HR), 3.35; 95% CI, 1.11–10.10; P = 0.03] and stage of primary tumor (HR, 6.26; 95% CI, 2.00–19.55; P < 0.01) were hazard factors for PRS and OS respectively.

Conclusions

Complete pulmonary resection is an acceptable option in selected patients with recurrent lung cancer after surgery. The patients with r-stage earlier than IIIA may benefit from completion pulmonary resection but not IIIB. Completion pneumonectomy failed to significantly prolong the OS. The OS in the enrolled cases was mainly affected by the p-TNM stage assessed by the first resection for primary lung cancer.
The risk factors of low anterior resection syndrome after colorectal cancer surgery: A retrospective study of 566 patients in a single institution in China
Authors: Ri HyokJu , Kang HaoNan , Xu ZhaoHui , Kim KunHyok , Ren YanYing , Gong ZeZhong , Chen Xin
Published: November 2022

Purpose

This study aims to identify the independent risk factors in the low anterior resection syndrome (LARS) after surgery for colorectal cancer (CRC).

Method

This was a retrospective, single-institution study in the Second Affiliation Hospital of Dalian Medical University, China. Patients underwent sphincter-preserving low anterior resection with total or partial mesorectal resection (with or without protective ileostomy) and completed a self-filled questionnaire over the phone to assess postoperative bowel dysfunction from January 2017 to December 2019. The predictors of LAR were evaluated using univariate and multivariate analyses.

Result

The study population was 566 patients, 264 (46.64%), 224 (39.58%), and 78 (13.78%) patients with no, minor, and major LARS, respectively. In the univariate analysis, independent factors such as tumor location and size, anastomotic height, protective ileostomy, post-operation chemoradiotherapy, tumor T stage, lymphatic nodal metastasis classification, surgery duration, and time interval for closure of stoma were significantly associated with LARS points while we found the tumor T stage and lymphatic nodal metastasis classification as the new independent risk factors compared with the last decade studies. In the multivariate analysis, factors such as low and middle tumor location and protective ileostomy, and post operation treatment, nodal metastasis classification were the independent risk factors for major LARS.

Conclusion

The new independence risk factors were tumor T stage and lymphatic nodal metastasis status in univariate analysis in our study, with anastomotic height, low and middle tumor location, protective ileostomy, post-operation chemoradiotherapy, nodal metastasis status increasing LARS point in multivariate analysis after surgery for CRC.
Quality and Nutritional Value of ‘Chopin’ and Clone ‘JB’ in Relation to Popular Apples Growing in Poland
Authors: Kistechok Andrii , Wrona Dariusz , Krupa Tomasz
Published: November 2022

The aim of the study was to describe the physicochemical properties, with particular emphasis on nutritional value, of apples ‘Chopin’ and clone ‘JB’. The new cultivars were compared with the cultivars commonly cultivated in Polish orchards, namely ‘Gala Brookfield’, ‘Šampion’, ‘Ligol’ and ‘Idared’. The study focuses on the evaluation of physicochemical characteristic (firmness, soluble solids content and titratable acidity) and the content of monosaccharides, sucrose and organic acids based on HPLC with an RI detector. In addition, the nutritional value of apples were described separately for the flesh and peel of the fruit, focusing on the assessment of the antioxidant activity and the content of total polyphenols, phenolic acids, flavonols using the HPLC technique. ‘Chopin’ and ‘JB’ clone apples are characterized by very high acidity, over 1%, which is related to the high content of malic acid. The red flesh ‘JB’ clone is characterized by a high content of bioactive compounds in both the peel and flesh of apples. High temperatures and a lack of precipitation contribute to a higher polyphenol content in apples, which proves that apart from the genetic features of the cultivars, the climatic conditions also determine the nutritional value of the fruit.
A Novel Approach of Combining Methylene Blue Photodynamic Inactivation, Photobiomodulation and Oral Ingested Methylene Blue in COVID-19 Management: A Pilot Clinical Study with 12-Month Follow-Up
Authors: Hepburn Juliette , Williams-Lockhart Susan , Bensadoun René Jean , Hanna Reem
Published: November 2022

Coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 virus was first recognized in late 2019 and remains a significant threat. We therefore assessed the use of local methylene blue photodynamic viral inactivation (MB-PDI) in the oral and nasal cavities, in combination with the systemic anti-viral, anti-inflammatory and antioxidant actions of orally ingested methylene blue (MB) and photobiomodulation (PBM) for COVID-19 disease. The proposed protocol leverages the separate and combined effects of MB and 660nm red light emitted diode (LED) to comprehensively address the pathophysiological sequelae of COVID-19. A total of eight pilot subjects with COVID-19 disease were treated in the Bahamas over the period June 2021–August 2021, using a remote care program that was developed for this purpose. Although not a pre-requisite for inclusion, none of the subjects had received any COVID-19 vaccination prior to commencing the study. Clinical outcome assessment tools included serial cycle threshold measurements as a surrogate estimate of viral load; serial online questionnaires to document symptom response and adverse effects; and a one-year follow-up survey to assess long-term outcomes. All subjects received MB-PDI to target the main sites of viral entry in the nose and mouth. This was the central component of the treatment protocol with the addition of orally ingested MB and/or PBM based on clinical requirements. The mucosal surfaces were irradiated with 660 nm LED in a continuous emission mode at energy density of 49 J/cm2 for PDI and 4.9 J/cm2 for PBM. Although our pilot subjects had significant co-morbidities, extremely high viral loads and moderately severe symptoms during the Delta phase of the pandemic, the response to treatment was highly encouraging. Rapid reductions in viral loads were observed and negative PCR tests were documented within a median of 4 days. These laboratory findings occurred in parallel with significant clinical improvement, mostly within 12–24 h of commencing the treatment protocol. There were no significant adverse effects and none of the subjects who completed the protocol required in-patient hospitalization. The outcomes were similarly encouraging at one-year follow-up with virtual absence of “long COVID” symptoms or of COVID-19 re-infection. Our results indicate that the protocols may be a safe and promising approach to challenging COVID-19 disease. Moreover, due its broad spectrum of activity, this approach has the potential to address the prevailing and future COVID-19 variants and other infections transmitted via the upper respiratory tract. Extensive studies with a large cohort are warranted to validate our results.
UAV Path Planning Model Based on R5DOS Model Improved A-Star Algorithm
Authors: Li Jian , Liao Changyi , Zhang Weijian , Fu Haitao , Fu Shengliang
Published: November 2022

In order to solve the problems of large amounts of calculation and long calculation times of the A-star algorithm in three-dimensional space, based on the R5DOS model, this paper proposes a three-dimensional space UAV path planning model. The improved R5DOS intersection model is combined with the improved A-star algorithm. Together, they construct a local search process, and the R5DOS path planning model is established by reducing the number of search nodes. The path planning model is simulated through MATLAB software and the model can greatly reduce the number of nodes and computational complexity of the A-star algorithm in three-dimensional spaces, while also reducing the calculation time of the UAV. Finally, we compare the improved A-star algorithm with the original A-star algorithm and the geometric A-star algorithm. The final fitting result proves that the improved A-star algorithm has a shorter computation time and fewer node visits. Overall, the simulation results confirm the effectiveness of the improved A-star algorithm and they can be used as a reference for future research on path planning algorithms.
A Joint Dispatch Operation Method of Hydropower and Photovoltaic: Based on the Two-Stage Hedging Model
Authors: Xie Tuo , Liu Hong , Zhang Gang , Zhang Kaoshe , Li Pai
Published: November 2022

The randomness and volatility of large-scale clean energy output represented by wind power and photovoltaic lead to difficulties in grid connection. The problems of abandoned wind, light, and water become increasingly prominent. The adjustment capacity of traditional thermal power is limited and it is difficult to ensure the consumption of high proportion clean energy. On this basis, the marginal benefit hedging rule in economics is introduced into the hydropower and photovoltaic joint operation system in this paper. A two-stage spatio-temporal hedging strategy is designed to solve the spatio-temporal conflict problem in the hydropower and photovoltaic joint system. The multi-objective joint dispatching model of hydropower and photovoltaic system considering system benefits, risks, and stability is established, which can be solved by a MOEA/D-GABS algorithm with selection strategy. The joint system scheduling schemes under different schemes are analyzed by case. The results demonstrate that, compared with the traditional multi-objective decision-making scheme, the flood control risk in each period of the reservoir in the proposed method is controlled to be no more than 1.63 × 10−3 (the flood control standard corresponding to the 50-year flood control risk is 0.006); the flood limit water level of the reservoir is increased from 583.00 m to 583.70 m, which improves the benefit of the reservoir; and the water utilization rate is effectively improved. On the other hand, compared with the traditional scheme, the proposed method reduces the peak valley difference of the combined system by 50.67% and 59.68% in typical sunny and cloudy scenarios, respectively, which greatly reduces the uncertainty of photovoltaic output, and the stability of the combined system is improved. It is shown that the proposed method can be used to guide the economic dispatch of a complementary system with hydropower as the regulating energy.
Global Existence of Bounded Solutions for Eyring–Powell Flow in a Semi-Infinite Rectangular Conduct
Authors: Rahman Saeed ur , Palencia Jose Luis Diaz , Tariq Nomaq , Salgado Sánchez Pablo , Gonzalez Julian Roa
Published: November 2022

The purpose of the present study is to obtain regularity results and existence topics regarding an Eyring–Powell fluid. The geometry under study is given by a semi-infinite conduct with a rectangular cross section of dimensions L×H. Starting from the initial velocity profiles (u10,u20) in xy-planes, the fluid flows along the z-axis subjected to a constant magnetic field and Dirichlet boundary conditions. The global existence is shown in different cases. First, the initial conditions are considered to be squared-integrable; this is the Lebesgue space (u10,u20)∈L2(Ω), Ω=[0,L]×[0,H]×(0,∞). Afterward, the results are extended for (u10,u20)∈Lp(Ω), p>2. Lastly, the existence criteria are obtained when (u10,u20)∈H1(Ω). A physical interpretation of the obtained bounds is provided, showing the rheological effects of shear thinningand shear thickening in Eyring–Powell fluids.
Local Grey Predictor Based on Cubic Polynomial Realization for Market Clearing Price Prediction
Authors: Saxena Akash , Alrasheedi Adel Fahad , Alnowibet Khalid Abdulaziz , Alshamrani Ahmad M. , Shekhawat Shalini , Mohamed Ali Wagdy
Published: November 2022

With the development of restructured power markets, the profit-making competitive business environment has emerged. With the help of different advanced technologies, generating companies are taking decisions regarding trading electricity with imperfect information about marketing operating conditions. The forecasting of the market clearing price (MCP) is a potential issue in these markets. Early information on the MCP can be a proven beneficial tool for accumulating profit. In this work, a local grey prediction model based on a cubic polynomial function is presented to estimate the MCP with the help of historical data. The mathematical framework of this grey model was established and evaluated for different market conditions and databases. The comparison between traditional grey models and some advanced grey models reveals that the proposed model yields accurate results.
A Transwell-Based Vascularized Model to Investigate the Effect of Interstitial Flow on Vasculogenesis
Authors: Deng Pengwei , Zhao Mengqian , Zhang Xu , Qin Jianhua
Published: November 2022

Interstitial flow plays a significant role in vascular system development, mainly including angiogenesis and vasculogenesis. However, compared to angiogenesis, the effect of interstitial flow on vasculogenesis is less explored. Current in vitro models for investigating the effect of interstitial flow on vasculogenesis heavily rely on microfluidic chips, which require microfluidic expertise and facilities, and may not be accessible to biological labs. Here, we proposed a facile approach to building perfusable vascular networks through the self-assembly of endothelial cells in a modified transwell format and investigated the effect of interstitial flow on vasculogenesis. We found that the effect of interstitial flow on vasculogenesis was closely related to the existence of VEGF and fibroblasts in the developed model: (1) In the presence of fibroblasts, interstitial flow (within the range of 0.1–0.6 μm/s) facilitated the perfusability of the engineered vasculatures. Additional VEGF in the culture medium further worked synergically with interstitial flow to develop longer, wider, denser, and more perfusable vasculatures than static counterparts; (2) In the absence of fibroblasts, vasculatures underwent severe regression within 7 days under static conditions. However, interstitial flow greatly inhibited vessel regression and enhanced vascular perfusability and morphogenesis without the need for additional VEGF. These results revealed that the effect of interstitial flow might vary depending on the existence of VEGF and fibroblasts, and would provide some guidelines for constructing in vitro self-assembled vasculatures. The established transwell-based vascularized model provides a simple method to build perfusable vasculatures and could also be utilized for creating functional tissues in regenerative medicine.
Embedded Electronic Sensor for Monitoring of Breathing Activity, Fitting and Filter Clogging in Reusable Industrial Respirators
Authors: Aqueveque Pablo , Díaz Macarena , Gomez Britam , Osorio Rodrigo , Pastene Francisco , Radrigan Luciano , Morales Anibal
Published: November 2022

Millions of workers are required to wear reusable respirators in several industries worldwide. Reusable respirators include filters that protect workers against harmful dust, smoke, gases, and vapors. These hazards may cause cancer, lung impairment, and diseases. Respiratory protection is prone to failure or misuse, such as wearing respirators with filters out of service life and employees wearing respirators loosely. Currently, there are no commercial systems capable of reliably alerting of misuse of respiratory protective equipment during the workday shifts or provide early information about dangerous clogging levels of filters. This paper proposes a low energy and non-obtrusive functional building block with embedded electronics that enable breathing monitoring inside an industrial reusable respirator. The embedded electronic device collects multidimensional data from an integrated pressure, temperature, and relative humidity sensor inside a reusable industrial respirator in real time and sends it wirelessly to an external platform for further processing. Here, the calculation of instantaneous breathing rate and estimation of the filter’s respirator fitting and clogging level is performed. The device was tested with ten healthy subjects in laboratory trials. The subjects were asked to wear industrial reusable respirator with the embedded electronic device attached inside. The signals measured with the system were compared with airflow signals measured with calibrated transducers for validation purposes. The correlation between the estimated breathing rates using pressure, temperature, and relative humidity with the reference signal (airflow) is 0.987, 0.988 and 0.989 respectively, showing that instantaneous breathing rate can be calculated accurately using the information from the embedded device. Moreover, respirator fitting (well-fitted or loose condition) and filter’s clogging levels (≤60%, 80% and 100% clogging) also can be estimated using features extracted from absolute pressure measurements combined to statistical analysis ANOVA models. These experimental outputs represent promising results for further development of data-driven prediction models using machine learning techniques to determine filters end-of-service life. Furthermore, the proposed system would collect relevant data for real-time monitoring of workers’ breathing conditions and respirator usage, helping to improve occupational safety and health in the workplace.
Apelin-13 Protects Neurons by Attenuating Early-Stage Postspinal Cord Injury Apoptosis In Vitro
Authors: Lin Taotao , Zhao Yujie , Guo Shengyu , Wu Zhengru , Li Wenwen , Wu Rongcan , Wang Zhenyu , Liu Wenge
Published: November 2022

Apelin is a 77-amino-acid peptide that is an endogenous ligand for the G protein-coupled receptor APJ (Apelin receptor, APJ). Apelin-13, as the most bioactive affinity fragment of apelin, plays a role in energy metabolism, myocardial ischemia-reperfusion injury, and the regulation of the inflammatory response during oxidative stress, but its role in spinal cord injury is still unclear. This research identified and verified the differential expression of apelin in rat spinal cord injured tissues and normal spinal cord tissues by transcriptome sequencing in vivo and proved that apelin-13 protects neurons by strengthening autophagy and attenuating early-stage postspinal cord injury apoptosis in vitro. After constructing the model concerning a rat spinal cord hemisection damage, transcriptome sequencing was performed on the injured and normal spinal cord tissues of rats, which identified the differentially expressed gene apelin, with qRT-PCR detecting the representative level of apelin. The oxygen-glucose deprivation (OGD) model of PC12 cells was constructed in vitro to simulate spinal cord injury. The OGD injury times were 2 h, 4 h, 6 h, 8 h, and 12 h, and the non-OGD injury group was used as the control. The expression of apelin at each time point was observed by Western blotting. The expression of apelin was the lowest in the 6 h OGD injury group (p < 0.05). Therefore, the OGD injury time of 6 h was used in subsequent experiments. The noncytotoxic drug concentration of apelin-13 was determined with a Cell Counting Kit-8 (CCK-8) assay. An appropriate dose of apelin-13 (1 μM) significantly improved cell survival (p < 0.05). Thus, subsequent experiments selected a concentration of 1 μM apelin-13 as it significantly increased cell viability. Finally, we divided the experimental groups into four groups according to whether they received drugs (1 μM apelin-13, 24 h) or OGD (6 h): (1) control group: without apelin-13 or OGD injury; (2) apelin-13 group: with apelin-13 but no OGD injury; (3) OGD group: with OGD injury but without apelin-13; and (4) OGD + apelin-13 group: with apelin-13 and OGD injury. The TUNEL assay and flow cytometry results showed that compared with the OGD group, apoptosis in the OGD+Apelin-13 group was significantly reduced (p < 0.001). Determination of cell viability under different conditions by CCK-8 assay results displays that Apelin-13 can significantly improve the cell viability percentage under OGD conditions (p < 0.001). Western blotting results showed that apelin-13 decreased the expression ratios of apoptosis-related proteins Bax/Bcl-2 and cleaved-caspase3/caspase3 (p < 0.05), increasing the key to Beclin1-dependent autophagy pathway expression of the protein Beclin1. This finding indicates that apelin-13 protects neurons by strengthening autophagy and attenuating early-stage postspinal cord injury apoptosis in vitro.
Opposing Roles of IGFBP-3 and Heparanase in Regulating A549 Lung Cancer Cell Survival
Authors: Al Khashali Hind , Wareham Jadziah , Ray Ravel , Haddad Ben , Coleman Kai-Ling , Ranzenberger Robert , McCombs Patrick , Guthrie Jeffrey , Heyl Deborah , Evans Hedeel Guy
Published: November 2022

In this study, we examined the roles of heparanase and IGFBP-3 in regulating A549 and H1299 non-small-cell lung cancer (NSCLC) survival. We found that H1299 cells, known to be p53-null with no expression of IGFBP-3, had higher heparanase levels and activity and higher levels of heparan sulfate (HS) in the media compared to the media of A549 cells. Inhibiting heparanase activity or its expression using siRNA had no effect on the levels of IGFBP-3 in the media of A549 cells, reduced the levels of soluble HS fragments, and led to decreased interactions between IGFBP-3 and HS in the media. HS competed with HA for binding to IGFBP-3 or IGFBP-3 peptide (215-KKGFYKKKQCRPSKGRKR-232) but not the mutant peptide (K228AR230A). HS abolished the cytotoxic effects of IGFBP-3 but not upon blocking HA–CD44 signaling with the anti-CD44 antibody (5F12). Blocking HA–CD44 signaling decreased the levels of heparanase in the media of both A549 and H1299 cell lines and increased p53 activity and the levels of IGFBP-3 in A549 cell media. Knockdown of p53 led to increased heparanase levels and reduced IGFBP-3 levels in A549 cell media while knockdown of IGFBP-3 in A549 cells blocked p53 activity and increased heparanase levels in the media.
Distinct Dynamics of Migratory Response to PD-1 and CTLA-4 Blockade Reveals New Mechanistic Insights for Potential T-Cell Reinvigoration following Immune Checkpoint Blockade
Authors: Safaeifard Fateme , Goliaei Bahram , Aref Amir R. , Foroughmand-Araabi Mohammad-Hadi , Goliaei Sama , Lorch Jochen , Jenkins Russell W. , Barbie David A. , Shariatpanahi Seyed Peyman , Rüegg Curzio
Published: November 2022

Cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) and programmed cell death protein 1 (PD-1), two clinically relevant targets for the immunotherapy of cancer, are negative regulators of T-cell activation and migration. Optimizing the therapeutic response to CTLA-4 and PD-1 blockade calls for a more comprehensive insight into the coordinated function of these immune regulators. Mathematical modeling can be used to elucidate nonlinear tumor–immune interactions and highlight the underlying mechanisms to tackle the problem. Here, we investigated and statistically characterized the dynamics of T-cell migration as a measure of the functional response to these pathways. We used a previously developed three-dimensional organotypic culture of patient-derived tumor spheroids treated with anti-CTLA-4 and anti-PD-1 antibodies for this purpose. Experiment-based dynamical modeling revealed the delayed kinetics of PD-1 activation, which originates from the distinct characteristics of PD-1 and CTLA-4 regulation, and followed through with the modification of their contributions to immune modulation. The simulation results show good agreement with the tumor cell reduction and active immune cell count in each experiment. Our findings demonstrate that while PD-1 activation provokes a more exhaustive intracellular cascade within a mature tumor environment, the time-delayed kinetics of PD-1 activation outweighs its preeminence at the individual cell level and consequently confers a functional dominance to the CTLA-4 checkpoint. The proposed model explains the distinct immunostimulatory pattern of PD-1 and CTLA-4 blockade based on mechanisms involved in the regulation of their expression and may be useful for planning effective treatment schemes targeting PD-1 and CTLA-4 functions.
The Role of the Synthesis Routes on the CO-Sensing Mechanism of NiO-Based Gas Sensors
Authors: Stanoiu Adelina , Ghica Corneliu , Mihalcea Catalina Gabriela , Ghica Daniela , Simion Cristian Eugen
Published: November 2022

In this study, two alternative synthesis routes have been used in obtaining gas-sensitive NiO materials. The structural and morphological aspects were systematically investigated by X-ray diffraction (XRD) and transmission electron microscopy (TEM), revealing significant differences further mirrored in their sensing performances. Simultaneous electrical resistance and contact potential differences have been involved aiming to decouple the energetic contributions: work function (ΔΦ), surface band bending (qΔVs) and electron affinity (Δχ). Two sensing mechanism scenarios explained the enhancement and downgrading in the sensor response to carbon monoxide (CO) concerning the synthesis strategies. The role of relative humidity (RH) was considered throughout the electrical operando (in-field) investigations.
Longitudinal Analysis of Adolescent Adjustment: The Role of Attachment and Emotional Competence
Authors: Jiménez-Rodríguez Tamara , De la Barrera Usue , Schoeps Konstanze , Valero-Moreno Selene , Montoya-Castilla Inmaculada
Published: November 2022

Emotional competencies as well as secure attachment relationships with peers are protective factors that facilitate psychological adjustment among adolescents. In this study, we will analyse how these socio-emotional factors influence adolescents’ emotional symptoms, conduct problems and peer problems. The participants were 815 Spanish adolescents aged 12–17 years (M = 13.69; SD = 1.21) who completed the Strengths and Difficulties Questionnaire (SDQ), the Parent and Peer Attachment Inventory (IPPA) and the Emotional Skills and Competence Questionnaire (ESCQ). Besides descriptive analyses, such as Pearson’s bivariate correlations, two different methodologies were combined to predict adolescent adjustment: structural equation modelling (SEM) and fuzzy set qualitative comparative analysis (fsQCA). The results show that secure attachment with peers and high emotional competence predict better psychological adjustment (low levels of emotional symptoms, conduct problems and peer problems), while insecure peer attachment and low emotional competence predict maladjustment. These results emphasise the role of socio-emotional variables in the promotion of psychological adjustment in adolescence through the implementation of emotional education programs.
Clinical Validation of a New Optical Biometer for Myopia Control in a Healthy Pediatric Population
Authors: Martínez-Plaza Elena , Molina-Martín Ainhoa , Arias-Puente Alfonso , Piñero David P.
Published: November 2022

To assess the clinical validation of the Myah device in a pediatric population by evaluating the repeatability of biometric evaluations and analyzing its agreement with the Myopia Master system. A total of 51 children (51 eyes) were enrolled. Repeated measurements of flat (K1) and steep (K2) corneal radius, white-to-white (WTW) distance and axial length (AL) were performed with the Myah device. The same parameters were obtained from a subgroup (30 eyes) with the Myopia Master for the agreement analysis. The repeatability was assessed using the intrasubject standard deviation (Sw) and the intraclass correlation coefficient (ICC). The agreement was analyzed using the Bland–Altman method and the paired Student t-test. The Sw was 0.018 D, 0.021 D, 0.071 mm and 0.017 mm for K1, K2, WTW and AL, respectively (ICC ≥ 0.971). The mean difference and limits of agreement when comparing instruments were −0.013 (−0.102/0.077) for K1 (p = 0.16), −0.058 (−0.127/0.012) for K2 (p < 0.001), 0.151 (−0.370/0.673) for WTW (p < 0.001) and 0.030 (−0.091/0.151) for AL (p = 0.009). In conclusion, the Myah device provides consistent measurements of corneal radius, WTW distance and AL in a healthy pediatric population, validating their usefulness in clinical practice. These measurements could be used interchangeably with those provided by the Myopia Master device, although with some caution.
An Evaluation of Health-Related Quality of Life in Children with Nasal Septum Deviation
Authors: Chmielik Lechosław Paweł , Mielnik-Niedzielska Grażyna , Kasprzyk Anna , Stankiewicz Tomasz , Niedzielski Artur
Published: November 2022

Background: From the 1950s, the quality of life criterion came to be studied in earnest, originally forming a part of measurement of human development in Western Europe and the USA. The present study aims to compare the health-related quality of life (HRQL) between children with nasal septum deviation and healthy children controls. Materials and Methods: Subjects were children suffering from nasal septum deviation, one of the commonest chronic diseases of the upper respiratory tract. Controls were randomly recruited from kindergarten, primary and secondary schools (junior high school & high school). All schools and subjects were randomly selected. The CHQ-PF50 questionnaire was used and outcome scores were calculated by an algorithm for the 13 tested HRQL variables. Results: Means for all outcome scores in the test subjects (i.e., children with deviated nasal septums) varied between 3.65–89.27 with a standard deviation between 0.83–25.66 and a median between 3.4–100 (n = 101). Those for the controls (n = 102) were 3.78–97.11, 0.86–14.21 and 4.40–100, respectively. Test subjects showed significant scoring declines in Physical Fitness, Role/Social–Emotional/Behavioral, Role/Social–Physical, Mental Health, Self-esteem, General Health Perceptions, Parental Impact Emotional and Time and Family Limitations in Activities. Conclusions: 1. The well-being of children with nasal septum deviation was found to be chiefly limited by their physical fitness, effects of physical condition on social behavior/interaction and how health is perceived. 2. Parents considered their children’s health to be paramount, as demonstrated by assessing the HRQL.
An Experimental Study on Strengthening Recycled Aggregates Considering Dry Mixing before Slurry Coating
Authors: Li Xiaomeng , Fan Liujun , Chai Shupeng
Published: November 2022

The strengthening of recycled aggregates is a critical issue, as the low strength of recycled aggregates is the main reason that limits their widespread use. The slurry coating method can strengthen the recycled aggregates by repairing the aggregate surface, but it is hard to improve the internal strength due to the existence of pores and cracks. In this study, a new methodology considering dry mixing with fines to fill and bond the internal pores and cracks before slurry coating is proposed. Twelve strengthened samples considering different combinations of dry-mixing fines and coating solutions were prepared, and the basic physical and mechanical properties were compared, including the water-absorption rate, crushing value, and apparent density with unstrengthened aggregates. The results indicate that the proposed methodology can change the water-absorption rate significantly and improve the crushing resistance and apparent density of the recycled aggregates. A high correlation between the apparent density and the crushing value was also observed. Furthermore, the strengthening mechanism of dry mixing was also investigated by scanning electron microscopy. The micromorphology of the strengthened aggregates indicates that internal pores and cracks can be filled by dry mixing fines and then bonded together after hydration.
Nasal Mycology of Chronic Rhinosinusitis Revealed by Nanopore Sequencing
Authors: Jiang Rong-San , Shih Chien-Hung , Jiang Yu-Han , Hsieh Han-Hsueh , Chiang Yi-Fang , Chuang Han-Ni , Hsiao Tzu-Hung
Published: November 2022

Background: Nanopore sequencing (NS) is a third-generation sequencing technology capable of generating reads of long sequences. In this study, we used NS to investigate nasal mycology in patients with chronic rhinosinusitis (CRS). Methods: Nasal cavities of 13 CRS patients were individually irrigated with 20 mL of distilled water. The irrigant was forcefully blown by the patient into a basin. The collected fluid was placed into a centrifuge tube and processed using the method of Ponikau et al. The collected specimens were used for traditional fungal culture and sequenced for total DNA using NS. Results: Traditional fungal culture successfully grew fungi in the specimens of 11 (84.6%) patients. Aspergillus sp. and Penicillium sp. were found in four (30.8%) patients, Cladosporium sp. in three (23.1%) patients, and Candida albicans, Mucor sp. and Chaetomium sp. in one patient. NS revealed fungi abundance ranged from 81 to 2226, with the Shannon species diversity ranging from 1.094 to 1.683 at the genus level. Malassezia sp. was sequenced in 13 patients, Aspergillus sp. in 12 (92.3%) patients, Candida albicans in 11 (84.6%) patients, and Penicillium sp. in 10 (76.9%) patients. Conclusion: Our results showed that NS was sensitive and fast in detecting nasal fungi in CRS patients.
Ultrasound Radiomics for the Detection of Early-Stage Liver Fibrosis
Authors: Al-Hasani Maryam , Sultan Laith R. , Sagreiya Hersh , Cary Theodore W. , Karmacharya Mrigendra B. , Sehgal Chandra M.
Published: November 2022

Objective: The study evaluates quantitative ultrasound (QUS) texture features with machine learning (ML) to enhance the sensitivity of B-mode ultrasound (US) for the detection of fibrosis at an early stage and distinguish it from advanced fibrosis. Different ML methods were evaluated to determine the best diagnostic model. Methods: 233 B-mode images of liver lobes with early and advanced-stage fibrosis induced in a rat model were analyzed. Sixteen features describing liver texture were measured from regions of interest (ROIs) drawn on B-mode images. The texture features included a first-order statistics run length (RL) and gray-level co-occurrence matrix (GLCM). The features discriminating between early and advanced fibrosis were used to build diagnostic models with logistic regression (LR), naïve Bayes (nB), and multi-class perceptron (MLP). The diagnostic performances of the models were compared by ROC analysis using different train-test sampling approaches, including leave-one-out, 10-fold cross-validation, and varying percentage splits. METAVIR scoring was used for histological fibrosis staging of the liver. Results: 15 features showed a significant difference between the advanced and early liver fibrosis groups, p < 0.05. Among the individual features, first-order statics features led to the best classification with a sensitivity of 82.1–90.5% and a specificity of 87.1–89.8%. For the features combined, the diagnostic performances of nB and MLP were high, with the area under the ROC curve (AUC) approaching 0.95–0.96. LR also yielded high diagnostic performance (AUC = 0.91–0.92) but was lower than nB and MLP. The diagnostic variability between test-train trials, measured by the coefficient-of-variation (CV), was higher for LR (3–5%) than nB and MLP (1–2%). Conclusion: Quantitative ultrasound with machine learning differentiated early and advanced fibrosis. Ultrasound B-mode images contain a high level of information to enable accurate diagnosis with relatively straightforward machine learning methods like naïve Bayes and logistic regression. Implementing simple ML approaches with QUS features in clinical settings could reduce the user-dependent limitation of ultrasound in detecting early-stage liver fibrosis.
The Moderating Effect of Corporate Governance on Corporate Social Responsibility and Information Asymmetry: An Empirical Study of Chinese Listed Companies
Authors: Alduais Fahd , Almasria Nashat Ali , Airout Rana
Published: November 2022

This study is conducted to investigate the relationship between corporate social responsibility (CSR) and information asymmetry (IA), as well as the role of corporate governance (CG) as a moderating factor. This paper employs panel data regression analysis. The CSR disclosure scores are collected from the HX database by way of Hexun.com, while financial data are collected from the CSMAR database. The association between CSR and information asymmetry is examined using generalised least squares (GLS). The current evidence shows that CSR disclosure reduces information asymmetry. In addition, the findings illustrate that particular aspects of CG moderate the relationship between CSR and information asymmetry. More specifically, board size, CEO duality, and board independence positively affect the bid–ask spread. Moderation by the independence board positively affects the relationship between CSR disclosure and information asymmetry. Since the sample is derived from large Chinese companies, the results should be supported by samples obtained from the COVID-19 pandemic in 2020 and, subsequently, comparisons with the entire stock market. In future studies, we recommend conducting research using other variables as proxies regarding information asymmetry. The current study extends existing research on CSR and IA by adding both board characteristics and ownership concentration variables as moderating variables.
Quiescent Optical Solitons with Cubic–Quartic and Generalized Cubic–Quartic Nonlinearity
Authors: Arnous Ahmed H. , Biswas Anjan , Yıldırım Yakup , Moraru Luminita , Moldovanu Simona , Moshokoa Seithuti P.
Published: November 2022

The enhanced Kudryashov’s approach retrieves quiescent bright, dark, and singular solitons to the governing model that is considered with cubic–quartic form of self-phase modulation. The algorithm however fails to retrieve stationary solitons when the nonlinearity is the generalized version of the cubic–quartic form. The current analysis is conducted with a direct approach without an intermediary phase-portrait analysis as in the past.
An 11-Bit 10 MS/s SAR ADC with C–R DAC Calibration and Comparator Offset Calibration
Authors: Jung Hoyong , Youn Eunji , Jang Young-Chan
Published: November 2022

An 11-bit 10 MS/s successive approximation register (SAR) analog-to-digital converter (ADC) is proposed for low-power and small-area applications. A 10-bit differential capacitor–resistor (C–R) digital-to-analog converter (DAC) is used to minimize the area of a DAC. The use of a C–R DAC reduces the capacitor area of a SAR ADC used CDAC by 75%. A capacitor calibration for the upper 5-bit capacitors of the C–R DAC is proposed to increase the linearity of the C–R DAC. To evaluate the proposed SAR ADC, an 11-bit 10 MS/s SAR ADC is implemented using a 180 nm 1-poly six-metal CMOS process with a supply of 1.8 V. The proposed SAR ADC has an effective number of bits (ENOBs) of 10.3 bits at a sampling rate of 10 MS/s for a 3.6-VPP differential sinusoidal analog input with a frequency of 4.789 MHz. The measured ENOBs is 10.45 bits when the frequency of the analog input signal is 42.39 kHz. The proposed C–R DAC calibration including comparator offset calibration improves the performances of differential nonlinearity (DNL) and integral nonlinearity (INL) from −1/+1.26 LSBs and −1.98/+1.96 LSBs to −0.97/+0.85 LSBs and −0.79/+0.83 LSBs, respectively.
The Evolution of Hypovolemic and Euvolemic Hyponatremia Coincides with an Inflammatory Status in Patients with COVID-19: An Observational Cohort Study
Authors: Ruiz-Sánchez Jorge Gabriel , Chávez-Díaz Pamela , Cárdenas-Salas Jersy
Published: November 2022

We evaluated the hospital evolution of hyponatremia and inflammation markers in patients with coronavirus disease 2019 (COVID-19). The hospital evolutions of a cohort of adult patients with COVID-19 pneumonia and hyponatremia were retrospectively analyzed. Data of the admission day, 2nd–3rd and 7th–10th day of hospitalization, and of the discharge day were collected. Comparative and multivariate analyzes were developed, and Hazzard ratio (HR) with 95% confidence intervals (95% CI) were calculated. Of the 172 hospitalized patients with COVID-19, 49 of them (28.5%) had hyponatremia, which were analyzed. A total of 32/49 (65.3%) patients were male, and 22/49 (44.9%) euvolemic. Mean age: 69.9 ± 14.7 years. All patients had high inflammatory markers at admission. Of the total patients with hyponatremia at admission, only 26.2% remained hyponatremic at the 7th–10th day of hospitalization. Improvement in serum sodium (SNa) coincided with improvement in inflammatory markers during hospitalization, in both euvolemic and hypovolemic hyponatremic patients. A higher serum creatinine at admission was independently associated with mortality (HR: 12.23, 95% CI: 2 to 25.6) in hyponatremic COVID-19 patients. In conclusion, both hypovolemic and euvolemic hyponatremia in COVID-19 patients occurred in an inflammation status, and improved as inflammation decreased.
Preparation and Antioxidant Activity In Vitro of Fermented Tremella fuciformis Extracellular Polysaccharides
Authors: Huang Qian , Liu Yu , Deng Yongfei , Yang Bin , Guo Ruixue , Jin Xiaobao , Zhou Lin
Published: November 2022

This study was aimed at increasing the capacity of fermented Tremella fuciformis extracellular polysaccharides (TEPS) for possible functional food applications. Thus, strain varieties, fermentation parameters and purification conditions, and the in vitro antioxidant activities of purified EPS fractions were investigated. An EPS high-yield strain Tf526 was selected, and the effects of seven independent fermentation factors (time, temperature, initial pH, inoculum size, shaking speed, carbon, and nitrogen source) on the EPS yield were evaluated. By single factor optimization test, yeast extract and glucose were chosen as nitrogen sources and carbon sources, respectively, and with initial pH of 6.0, inoculum size of 8%, shaking speed of 150 rpm, and culture at 25 °C for 72 h, the optimal yield of TEPS reached 0.76 ± 0.03 mg/mL. Additionally, A-722MP resin showed the most efficient decoloration ratio compared to six other tested resins. Furthermore, optimal decoloration parameters of A-722MP resin were obtained as follows: decoloration time of 2 h, resins dosage of 2 g, and temperature of 30 °C. Decoloration ratio, deproteinization ratio, and polysaccharide retention ratio were 62.14 ± 2.3%, 81.21 ± 2.13%, and 73.42 ± 1.96%, respectively. Furthermore, the crude TEPS was extracted and four polysaccharide fractions were isolated and purified as Tf1-a, Tf1-b, Tf2, and Tf3 by the DEAE-Sepharose FF column and the Sephasryl S100 column. In general, the antioxidant activities of the Lf1-a and Lf1-b were lower compared with Vc at the concentration of 0.1 to 3 mg/mL, but the FRAP assay, DPPH scavenging activity, and hydroxyl radical scavenging activity analysis still revealed that Tf1-a and Tf1-b possess significant antioxidant activities in vitro. At the concentration of 3 mg/mL, the reducing power of Lf1-a and Lf1-b reached 0.86 and 0.70, the maximum DPPH radical were 54.23 ± 1.68% and 61.62 ± 2.73%, and the maximum hydroxyl radicals scavenging rates were 58.76 ± 2.58% and 45.81 ± 1.79%, respectively. Moreover, there were significant correlations (r > 0.8) among the selected concentrations and antioxidant activities of TEPS major fractions Tf1-a and Tf1-b. Therefore, it is expected that Tf1-a and Tf1-b polysaccharide fractions from fermented TEPS may serve as active ingredients in functional foods.
Relationships between Virulence Genes and Antibiotic Resistance Phenotypes/Genotypes in Campylobacter spp. Isolated from Layer Hens and Eggs in the North of Tunisia: Statistical and Computational Insights
Authors: Gharbi Manel , Kamoun Selim , Hkimi Chaima , Ghedira Kais , Béjaoui Awatef , Maaroufi Abderrazak
Published: November 2022

Globally, Campylobacter is a significant contributor to gastroenteritis. Efficient pathogens are qualified by their virulence power, resistance to antibiotics and epidemic spread. However, the correlation between antimicrobial resistance (AR) and the pathogenicity power of pathogens is complex and poorly understood. In this study, we aimed to investigate genes encoding virulence and AR mechanisms in 177 Campylobacter isolates collected from layer hens and eggs in Tunisia and to assess associations between AR and virulence characteristics. Virulotyping was determined by searching 13 virulence genes and AR-encoding genes were investigated by PCR and MAMA-PCR. The following genes were detected in C. jejuni and C. coli isolates: tet(O) (100%/100%), blaOXA-61 (18.82%/6.25%), and cmeB (100%/100%). All quinolone-resistant isolates harbored the Thr-86-Ile substitution in GyrA. Both the A2074C and A2075G mutations in 23S rRNA were found in all erythromycin-resistant isolates; however, the erm(B) gene was detected in 48.38% and 64.15% of the C. jejuni and C. coli isolates, respectively. The machine learning algorithm Random Forest was used to determine the association of virulence genes with AR phenotypes. This analysis showed that C. jejuni virulotypes with gene clusters encompassing the racR, ceuE, virB11, and pldA genes were strongly associated with the majority of phenotypic resistance. Our findings showed high rates of AR and virulence genes among poultry Campylobacter, which is a cause of concern to human health. In addition, the correlations of specific virulence genes with AR phenotypes were established by statistical analysis.