bluuebunny/crossref_metadata_2025 huggingface dataset:
DEPLOYMENT BEHAVIOR OF STENT IN TAPERED VESSELS WITH MULTIPLE STENOSES: EFFECT OF DIFFERENT IMPLANTATION PLANNING SYSTEMS
Authors: SHEN XIANG , LI HANQING , JIANG JIABAO , TIAN RUN , LU KAIKAI
Published: May 2023

In-stent restenosis (ISR), which is a common complication after stent intervention, affects the long-term outcome of stent intervention. Suitable implantation planning will effectively reduce the incidence of ISR. The aim of this study is to investigate the effect of different implantation planning on the mechanical behavior of stents in tapered vessels with multiple stenoses and select a better implantation planning. The finite element method (FEM) was used in this study. A different planning was designed as follows: (a) using a cylindrical balloon to deploy cylindrical stent (plan CC), (b) using the tapered balloon to deploy cylindrical stent (plan TC) and (c) using the tapered balloon to deploy tapered stent (plan TT). When the stent was expanded to the maximum diameter, the position of maximum vascular stress all appeared at distal plaque in three implantation plans. After balloon was deflated, the vascular stress in plan TC was the minimum. Using tapered balloon to deploy cylindrical stent has advantages in the tapered vessels with multiple stenoses and could reduce the probability of ISR. Moreover, this method could assess the mechanical properties of stent deployment in multiple stenoses and then select a better implantation planning.
Central hemodynamic response to activation of the vestibular sympathetic reflex with head-down rotation in healthy young adults
Authors: Pascual Justin , DeBlois Jacob P. , Ray Chester , Hametner Bernhard , Heffernan Kevin S. , Voss Margaret A.
Published: May 2023

Despite peripheral vasoconstriction, activation of the vestibular-sympathetic reflex results in a reduction in cardiac workload. Reduction in cardiac workload is likely due to reductions in pressure from wave reflections and reservoir pressure.
Traumatic Anterior Hip Dislocation in the Elderly: Description and Review of a Rare Trauma
Authors: Schopfer Quentin , Strasser Roland , Leumessi Eric Ngassom , Traverso Aurélien
Published: May 2023

Background. Traumatic hip dislocation (THD) is an orthopaedic emergency that requires rapid reduction. THD is generally encountered in high-energy trauma. THD with low-energy trauma is extremely rare, even more so in the elderly. Methods/Results. We report the case of a 72-year-old woman who presented to the emergency department with anterior superior left hip dislocation after a low-energy trauma. Results. The patient was initially treated with closed reduction. Because of recurring dislocation, closed reduction was performed a second time. Magnetic resonance imaging showed no soft tissue interposition. At 12 week follow-up, the patient complained of intractable hip pain and was treated with total hip arthroplasty. The post-operative course was uneventful with a return to pre-injury functional mobility. We also conducted a review of the literature with regard to anterior hip dislocation in the population aged 70 years or more. Conclusion. THD can be associated with significant morbidity. Time to reduction is considered essential in improving functional outcomes. In the case of poor functional outcomes, total hip arthroplasty should be considered.
Path and Mechanism of Industrial Internet Industry Promoting the Transformation and Upgrading of Small and Medium-sized Enterprises with Artificial Intelligence
Authors: He Yi
Published: May 2023

With the development of intelligent sensing and communication, industrial Internet of Things (IoT) networks have been widely applied in many application scenarios. As a typical application of industrial Internet of Things (IoT) networks, industrial Internet industry has attracted much attention from researchers. Specifically, in the transformation and upgrading of small enterprises in the Internet industry, their paths and mechanisms are very important. The correct path and mechanism of transformation and upgrading can promote national economic growth, strengthen market competition, enhance market vitality, and optimize economic structure. Small and medium-sized enterprises have always been the backbone of my country’s economic development, especially in terms of promoting employment, promoting technological innovation, increasing national taxes, and improving people’s livelihood. In this paper, when discussing the relationship between knowledge sharing and business performance of SMEs, the organization name is introduced as an intermediate variable. By combining theoretical research and empirical research, a theoretical model is established, research hypotheses are proposed, and the previous research questionnaire is used, by exploiting the intelligent sensing and communication. As a reference, a research questionnaire suitable for the reality and needs of this research was designed. On this basis, this article first has a deep understanding of the research status of comprehensive budget management at home and abroad and analyzes the innovations and shortcomings of this article in the analysis process, understands the mobile Internet, integrates budget management, and integrates information for budget management. It prove the feasibility of helping small and medium-sized enterprises to transform in the mobile Internet environment and analyzes the feasibility of implementation to help small and medium-sized enterprises; it also shows that the industrial Internet industry is helpful to the transformation of traditional industries and is conducive to the transformation and upgrading of enterprises, thereby solving the problem of high cost of building an information platform, reducing the workload of IT personnel and extending the budget Management time provides new ideas for promoting the transformation and upgrading of the Internet for small and medium-sized enterprises, which can increase the efficiency of the small and medium-sized enterprises by more than 40% and greatly increase their profits, through the intelligent sensing and communication. The research of this paper provides important guidance for the application of industrial IoT networks, especially for the intelligent sensing and communication.
Error Bounds for Approximations Using Multichannel Deep Convolutional Neural Networks with Downsampling
Authors: Liu Xinling , Hou Jingyao
Published: May 2023

Deep learning with specific network topologies has been successfully applied in many fields. However, what is primarily called into question by people is its lack of theoretical foundation investigations, especially for structured neural networks. This paper theoretically studies the multichannel deep convolutional neural networks equipped with the downsampling operator, which is frequently used in applications. The results show that the proposed networks have outstanding approximation and generalization ability of functions from ridge class and Sobolev space. Not only does it answer an open and crucial question of why multichannel deep convolutional neural networks are universal in learning theory, but it also reveals the convergence rates.
Patients’ Experiences in a Newly Established Clinic for Late Complications after Colorectal and Anal Cancer Treatment: A Qualitative Study
Authors: Thing Oggesen Birthe , Akbar Momena , Sjødin Hamberg Marie Louise , Thomsen Thordis , Kjaergaard Danielsen Anne , Rosenberg Jacob
Published: May 2023

Purpose. Treatment of late complications is not systematically provided in Denmark. We therefore established a clinic to treat patients’ late complications. With this study, we wanted to explore patients’ experiences with treatment and care in such a clinic, including their recommendations for the future organization and structure of the clinic. Methods. We conducted a qualitative semistructured interview study with 14 patients who had attended a late complication clinic after treatment for colorectal or anal cancer. We used a descriptive approach to describe the patients’ experiences. Results. We found four major categories: (1) benefitting from the late complication clinic, (2) confusion about which clinic they attended, (3) recommendations for the future of the clinic, and (4) preparation and delivery of the consultations. Patients benefitted from attending the late complication clinic, and some experienced complete relief from symptoms. Others did not, but they gained hope that they might be able to receive treatment in the future. Patients wished for more information about late complications, preferring that the most common symptoms were described along with patient-friendly treatment options. The patients were satisfied with telephone consultations, as they were easy to fit into a daily schedule, and patients found it easy to express themselves openly. Conclusions. Patients were satisfied with the late complication clinic as they felt it gave them a safety net. For the future, patients recommended provision of more information about late complications and possible treatments.
Figure S1 from DAB2IP Is a Bifunctional Tumor Suppressor That Regulates Wild-Type RAS and Inflammatory Cascades in KRAS Mutant Colon Cancer
Authors: Miller Abigail L. , Perurena Naiara , Gardner Alycia , Hinoue Toshinori , Loi Patrick , Laird Peter W. , Cichowski Karen
Published: May 2023

An immunoblot showing anti-apoptotic NF-kB target genes in shCNT and shDAB2IP HCT116 cells.
Data from BCA101 Is a Tumor-Targeted Bifunctional Fusion Antibody That Simultaneously Inhibits EGFR and TGFβ Signaling to Durably Suppress Tumor Growth
Authors: Boreddy Srinivas Reddy , Nair Reshmi , Pandey Prashant Kumar , Kuriakose Anshu , Marigowda Shivakumar Bhadravathi , Dey Chaitali , Banerjee Arindam , Kulkarni Hanumant , Sagar Milind , Krishn Shiv Ram , Rao Shruthi , AR Madhukara , Tiwari Vinita , Alke Bhavna , MV Prashantha Kumar , Shri Meena , Dhamne Chaitrali , Patel Sonal , Sharma Pinky , Periyasamy Sankar , Bhatnagar Jaya , Kuriakose Moni Abraham , Reddy Ram Bhupal , Suresh Amritha , Sreenivas Suma , Govindappa Nagaraja , Moole Praveen Reddy , Bughani Usha , Tan Seng-Lai , Nair Pradip
Published: May 2023

Abstract
The EGFR and TGFβ signaling pathways are important mediators of tumorigenesis, and cross-talk between them contributes to cancer progression and drug resistance. Therapies capable of simultaneously targeting EGFR and TGFβ could help improve patient outcomes across various cancer types. Here, we developed BCA101, an anti-EGFR IgG1 mAb linked to an extracellular domain of human TGFβRII. The TGFβ “trap” fused to the light chain in BCA101 did not sterically interfere with its ability to bind EGFR, inhibit cell proliferation, or mediate antibody-dependent cellular cytotoxicity. Functional neutralization of TGFβ by BCA101 was demonstrated by several in vitro assays. BCA101 increased production of proinflammatory cytokines and key markers associated with T-cell and natural killer–cell activation, while suppressing VEGF secretion. In addition, BCA101 inhibited differentiation of naïve CD4+ T cells to inducible regulatory T cells (iTreg) more strongly than the anti-EGFR antibody cetuximab. BCA101 localized to tumor tissues in xenograft mouse models with comparable kinetics to cetuximab, both having better tumor tissue retention over TGFβ “trap.” TGFβ in tumors was neutralized by approximately 90% in animals dosed with 10 mg/kg of BCA101 compared with 54% in animals dosed with equimolar TGFβRII-Fc. In patient-derived xenograft mouse models of head and neck squamous cell carcinoma, BCA101 showed durable response after dose cessation. The combination of BCA101 and anti-PD1 antibody improved tumor inhibition in both B16-hEGFR–expressing syngeneic mouse models and in humanized HuNOG-EXL mice bearing human PC-3 xenografts. Together, these results support the clinical development of BCA101 as a monotherapy and in combination with immune checkpoint therapy.
Significance:
The bifunctional mAb fusion design of BCA101 targets it to the tumor microenvironment where it inhibits EGFR and neutralizes TGFβ to induce immune activation and to suppress tumor growth.
Data from The m6A Reader YTHDF2 Promotes Bladder Cancer Progression by Suppressing RIG-I–Mediated Immune Response
Authors: Zhang Lei , Li Yuqing , Zhou Lingli , Zhou Houhong , Ye Liefu , Ou Tong , Hong Huaishan , Zheng Shiwen , Zhou Ziyu , Wu Kang , Yan Zeqin , Thiery Jean Paul , Cui Jun , Wu Song
Published: May 2023

Abstract
N6-Methyladenosine (m6A) is the most prevalent internal modification of mammalian mRNAs. Recent studies have shown that m6A methyltransferases METTL3 and METTL14 play important roles in urothelial bladder carcinoma (BLCA). To provide a more comprehensive understanding of the m6A regulatory landscape in bladder cancer, we investigated the role of YTHDF2, a crucial m6A reader, in BLCA. YTHDF2 was frequently upregulated at both the RNA and protein level in BLCA. Functionally, YTHDF2 promoted the proliferation and tumor growth of BLCA cells in vitro and in vivo, respectively. Integrative RNA sequencing and m6A sequencing analyses identified RIG-I as a downstream target of YTHDF2. Mechanistically, YTHDF2 bound to the coding sequence of DDX58 mRNA, which encodes RIG-I, and mediated its degradation in an m6A-dependent manner. Knockdown of RIG-I inhibited apoptosis and promoted the proliferation of BLCA cells. Depleting RIG-I was also able to reverse the effects of YTHDF2 deficiency. YTHDF2-deficient BLCA cells implanted orthotopically in recipient mice activated an innate immune response and promoted recruitment of CD8+ T lymphocytes into the tumor bed and the urothelium. Moreover, YTHDF2 deficiency enhanced the efficacy of Bacillus Calmette-Guérin immunotherapy treatment. This study reveals that YTHDF2 acts as an oncogene in BLCA. YTHDF2 inhibits RIG-I to facilitate immune evasion, supporting testing YTHDF2 inhibition in combination with immunotherapy.
Significance:
YTHDF2 regulates RIG-I–mediated innate immune signaling to support bladder cancer progression, highlighting the functional importance of m6A modifications in bladder cancer and uncovering therapeutic opportunities to improve patient outcomes.
Data from Dual Impact of IGF2 on Alveolar Stem Cell Function during Tobacco-Induced Injury Repair and Development of Pulmonary Emphysema and Cancer
Authors: Boo Hye-Jin , Min Hye-Young , Park Choon-Sik , Park Jong-Sook , Jeong Ji Yun , Lee Shin Yup , Kim Woo-Young , Lee Jae-Won , Oh Sei-Ryang , Park Rang-Woon , Lee Ho-Young
Published: May 2023

Abstract
Pulmonary emphysema is a destructive inflammatory disease primarily caused by cigarette smoking (CS). Recovery from CS-induced injury requires proper stem cell (SC) activities with a tightly controlled balance of proliferation and differentiation. Here we show that acute alveolar injury induced by two representative tobacco carcinogens, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone and benzo[a]pyrene (N/B), increased IGF2 expression in alveolar type 2 (AT2) cells to promote their SC function and facilitate alveolar regeneration. Autocrine IGF2 signaling upregulated Wnt genes, particularly Wnt3, to stimulate AT2 proliferation and alveolar barrier regeneration after N/B-induced acute injury. In contrast, repetitive N/B exposure provoked sustained IGF2-Wnt signaling through DNMT3A-mediated epigenetic control of IGF2 expression, causing a proliferation/differentiation imbalance in AT2s and development of emphysema and cancer. Hypermethylation of the IGF2 promoter and overexpression of DNMT3A, IGF2, and the Wnt target gene AXIN2 were seen in the lungs of patients with CS-associated emphysema and cancer. Pharmacologic or genetic approaches targeting IGF2-Wnt signaling or DNMT prevented the development of N/B-induced pulmonary diseases. These findings support dual roles of AT2 cells, which can either stimulate alveolar repair or promote emphysema and cancer depending on IGF2 expression levels.
Significance:
IGF2-Wnt signaling plays a key role in AT2-mediated alveolar repair after cigarette smoking–induced injury but also drives pathogenesis of pulmonary emphysema and cancer when hyperactivated.
A Designer Strategy to Develop Novel Bispecific Cancer Therapeutic Antibodies
Authors: Kumar Rakesh
Published: May 2023

Therapeutic antibodies selectively targeting EPHA2 with or without co-targeting another receptor tyrosine kinase have been limited to date. By integrating state-of-art proteogenomic, ex vivo models, and short hairpin RNA screening approaches, a new designing strategy has now discovered a bispecific therapeutic antibody co-targeting EPHA2 and EGFR – which effectively inhibits tumor cell growth in various preclinical cancer models. This new antibody provides new tools to impair the acquired resistance to EGFR-directed therapies or co-target EPHA2 and EGFR in human tumor.

See related article by El Zawily et al., p. 2686
Supplementary Online Material from 3D-Informed Targeting of the Trop-2 Signal-Activation Site Drives Selective Cancer Vulnerability
Authors: Guerra Emanuela , Trerotola Marco , Relli Valeria , Lattanzio Rossano , Tripaldi Romina , Ceci Martina , Boujnah Khouloud , Pantalone Ludovica , Sacchetti Andrea , Havas Kristina M. , Simeone Pasquale , Travali Nicole , Querzoli Patrizia , Pedriali Massimo , Roversi Pietro , Iezzi Manuela , Tinari Nicola , Antolini Laura , Alberti Saverio
Published: May 2023

Supplementary Online Material
Data from 3D-Informed Targeting of the Trop-2 Signal-Activation Site Drives Selective Cancer Vulnerability
Authors: Guerra Emanuela , Trerotola Marco , Relli Valeria , Lattanzio Rossano , Tripaldi Romina , Ceci Martina , Boujnah Khouloud , Pantalone Ludovica , Sacchetti Andrea , Havas Kristina M. , Simeone Pasquale , Travali Nicole , Querzoli Patrizia , Pedriali Massimo , Roversi Pietro , Iezzi Manuela , Tinari Nicola , Antolini Laura , Alberti Saverio
Published: May 2023

Abstract
Next-generation Trop-2–targeted therapy against advanced cancers is hampered by expression of Trop-2 in normal tissues. We discovered that Trop-2 undergoes proteolytic activation by ADAM10 in cancer cells, leading to the exposure of a previously inaccessible protein groove flanked by two N-glycosylation sites. We designed a recognition strategy for this region, to drive selective cancer vulnerability in patients. Most undiscriminating anti–Trop-2 mAbs recognize a single immunodominant epitope. Hence, we removed it by deletion mutagenesis. Cancer-specific, glycosylation-prone mAbs were selected by ELISA, bio-layer interferometry, flow cytometry, confocal microscopy for differential binding to cleaved/activated, wild-type and glycosylation site–mutagenized Trop-2. The resulting 2G10 mAb family binds Trop-2–expressing cancer cells, but not Trop-2 on normal cells. We humanized 2G10 by state-of-the-art complementarity determining region grafting/re-modeling, yielding Hu2G10. This antibody binds cancer-specific, cleaved/activated Trop-2 with Kd < 10−12 mol/L, and uncleaved/wtTrop-2 in normal cells with Kd 3.16×10−8 mol/L, thus promising an unprecedented therapeutic index in patients. In vivo, Hu2G10 ablates growth of Trop-2–expressing breast, colon, prostate cancers, but shows no evidence of systemic toxicity, paving the way for a paradigm shift in Trop-2–targeted therapy.
Table S4 from MYC Induces Immunotherapy and IFNγ Resistance Through Downregulation of JAK2
Authors: Markovits Ettai , Harush Ortal , Baruch Erez N. , Shulman Eldad D. , Debby Assaf , Itzhaki Orit , Anafi Liat , Danilevsky Artem , Shomron Noam , Ben-Betzalel Guy , Asher Nethanel , Shapira-Frommer Ronnie , Schachter Jacob , Barshack Iris , Geiger Tamar , Elkon Ran , Besser Michal J. , Markel Gal
Published: May 2023

Similarity between TIL ACT and PD-1 blockade response mechanism. Related to Figure S1.
Table S3 from MYC Induces Immunotherapy and IFNγ Resistance Through Downregulation of JAK2
Authors: Markovits Ettai , Harush Ortal , Baruch Erez N. , Shulman Eldad D. , Debby Assaf , Itzhaki Orit , Anafi Liat , Danilevsky Artem , Shomron Noam , Ben-Betzalel Guy , Asher Nethanel , Shapira-Frommer Ronnie , Schachter Jacob , Barshack Iris , Geiger Tamar , Elkon Ran , Besser Michal J. , Markel Gal
Published: May 2023

Comparison between proteome and transcriptome profiling. Related to Figure 1.
Supplementary Figure Legends from MYC Induces Immunotherapy and IFNγ Resistance Through Downregulation of JAK2
Authors: Markovits Ettai , Harush Ortal , Baruch Erez N. , Shulman Eldad D. , Debby Assaf , Itzhaki Orit , Anafi Liat , Danilevsky Artem , Shomron Noam , Ben-Betzalel Guy , Asher Nethanel , Shapira-Frommer Ronnie , Schachter Jacob , Barshack Iris , Geiger Tamar , Elkon Ran , Besser Michal J. , Markel Gal
Published: May 2023

Supplementary Figure Legends
Supplemental Table 3 from mWTX-330, an IL-12 INDUKINE Molecule, Activates and Reshapes Tumor-Infiltrating CD8+ T and NK Cells to Generate Antitumor Immunity
Authors: Nirschl Christopher J. , Brodkin Heather R. , Domonkos Celesztina , Dwyer Connor J. , Hicklin Daniel J. , Ismail Nesreen , Seidel-Dugan Cynthia , Steiner Philipp , Steuert Zoe , Sullivan Jenna M. , Winston William M. , Salmeron Andres
Published: May 2023

Differential Gene Expression Analysis of EMT-6 Tumor Infiltrating CD8+ T cells
Supplementary Figure 6 from MYC Induces Immunotherapy and IFNγ Resistance Through Downregulation of JAK2
Authors: Markovits Ettai , Harush Ortal , Baruch Erez N. , Shulman Eldad D. , Debby Assaf , Itzhaki Orit , Anafi Liat , Danilevsky Artem , Shomron Noam , Ben-Betzalel Guy , Asher Nethanel , Shapira-Frommer Ronnie , Schachter Jacob , Barshack Iris , Geiger Tamar , Elkon Ran , Besser Michal J. , Markel Gal
Published: May 2023

Supplementary Figure 6
Data from mWTX-330, an IL-12 INDUKINE Molecule, Activates and Reshapes Tumor-Infiltrating CD8+ T and NK Cells to Generate Antitumor Immunity
Authors: Nirschl Christopher J. , Brodkin Heather R. , Domonkos Celesztina , Dwyer Connor J. , Hicklin Daniel J. , Ismail Nesreen , Seidel-Dugan Cynthia , Steiner Philipp , Steuert Zoe , Sullivan Jenna M. , Winston William M. , Salmeron Andres
Published: May 2023

Abstract
IL-12 is a pleotropic inflammatory cytokine, which has broad stimulatory effects on various immune cell populations, making it an attractive target for cancer immunotherapy. However, despite generating robust antitumor activity in syngeneic murine tumor models, clinical administration of IL-12 has been limited by severe toxicity. mWTX-330 is a selectively inducible INDUKINE molecule comprised of a half-life extension domain and an inactivation domain linked to chimeric IL-12 by tumor protease–sensitive linkers. Systemic administration of mWTX-330 in mice was well tolerated, resulted in robust antitumor immunity in multiple tumor models, and preferentially activated tumor-infiltrating immune cells rather than immune cells present in peripheral tissues. Antitumor activity was dependent on in vivo processing of the protease cleavable linkers and required CD8+ T cells for full efficacy. Within the tumor, mWTX-330 increased the frequency of cross-presenting dendritic cells (DC), activated natural killer (NK) cells, skewed conventional CD4+ T cells toward a T helper 1 (TH1) phenotype, drove regulatory T cells (Treg) fragility, and increased the frequency of polyfunctional CD8+ T cells. mWTX-330 treatment also increased the clonality of tumor-infiltrating T cells by expanding underrepresented T-cell receptor (TCR) clones, drove CD8+ T and NK cells towards increased mitochondrial respiration and fitness, and decreased the frequency of TOX+ exhausted CD8+ T cells within the tumor. A fully human version of this INDUKINE molecule was stable in human serum, was reliably and selectively processed by human tumor samples, and is currently in clinical development.
Table S1 from Deconstructing olfactory epithelium developmental pathways in olfactory neuroblastoma
Authors: Finlay John B , Abi Hachem Ralph , Jang David W , Osazuwa-Peters Nosayaba , Goldstein Bradley J
Published: May 2023

Tumor demographics separated by Hyams grade, Dulguerov T stage, and gene expression groups (low versus high) used for survival analysis in Figure 5 and Figure S4. Average Ki-67 mRNA expression obtained from bulk RNA-Seq outputs. Average Ki-67 index obtained from histology data reported by Classe et al., 2018. +/- in the last two columns is standard error of the mean (SEM).
Figure S4 from Deconstructing olfactory epithelium developmental pathways in olfactory neuroblastoma
Authors: Finlay John B , Abi Hachem Ralph , Jang David W , Osazuwa-Peters Nosayaba , Goldstein Bradley J
Published: May 2023

Additional Kaplan-Meier survival curves based on Hyams Grade, Dulguerov T-stage, and MKI67 gene expression. Log-rank test used to determine statistical significance.
Figure S3 from Deconstructing olfactory epithelium developmental pathways in olfactory neuroblastoma
Authors: Finlay John B , Abi Hachem Ralph , Jang David W , Osazuwa-Peters Nosayaba , Goldstein Bradley J
Published: May 2023

Identical RNA expression plots to those shown in Fig. 4 A and B, but with IDH2 mutant ONB separated out from wild type tumors.
Figure S2 from Deconstructing olfactory epithelium developmental pathways in olfactory neuroblastoma
Authors: Finlay John B , Abi Hachem Ralph , Jang David W , Osazuwa-Peters Nosayaba , Goldstein Bradley J
Published: May 2023

RNA expression signatures of ONB from Classe et al., 2018 based on markers traditionally associated with sinonasal undifferentiated carcinoma (A), small cell neuroendocrine carcinoma (B), and olfactory neuroblastoma (C), divided by control OE, low grade ONB, high grade ONB, and IDH2 mutant ONB. A) Expression of keratins is not significantly increased in IDH2 mutant ONB versus IDH2 wild type tumors. B) Small cell neuroendocrine carcinoma (SNEC) marker genes are not highly expressed ONB from Classe et al. dataset. C) Expression of canonical neuroendocrine markers is negatively associated with ONB grade, and is not significantly different between high grade IDH2 and high grade IDH2 mutant ONB.
Supplementary Table S3 from Chemokine Analysis in Patients with Metastatic Uveal Melanoma Suggests a Role for CCL21 Signaling in Combined Epigenetic Therapy and Checkpoint Immunotherapy
Authors: Sah Vasu R. , Jespersen Henrik , Karlsson Joakim , Nilsson Lisa M. , Bergqvist Mattias , Johansson Iva , Carneiro Ana , Helgadottir Hildur , Levin Max , Ullenhag Gustav , Ståhlberg Anders , Olofsson Bagge Roger , Nilsson Jonas A. , Ny Lars
Published: May 2023

Patient sample information
Figure S6 from Chemokine Analysis in Patients with Metastatic Uveal Melanoma Suggests a Role for CCL21 Signaling in Combined Epigenetic Therapy and Checkpoint Immunotherapy
Authors: Sah Vasu R. , Jespersen Henrik , Karlsson Joakim , Nilsson Lisa M. , Bergqvist Mattias , Johansson Iva , Carneiro Ana , Helgadottir Hildur , Levin Max , Ullenhag Gustav , Ståhlberg Anders , Olofsson Bagge Roger , Nilsson Jonas A. , Ny Lars
Published: May 2023

Figure S6. Analysis of clinical parameters gender (a), tumor BAP1 status (b) and LDH levels (c-d) compared to serum levels of CCL21 (a-c) or presence of TLS-like regions in tumors.
Figure S5 from Chemokine Analysis in Patients with Metastatic Uveal Melanoma Suggests a Role for CCL21 Signaling in Combined Epigenetic Therapy and Checkpoint Immunotherapy
Authors: Sah Vasu R. , Jespersen Henrik , Karlsson Joakim , Nilsson Lisa M. , Bergqvist Mattias , Johansson Iva , Carneiro Ana , Helgadottir Hildur , Levin Max , Ullenhag Gustav , Ståhlberg Anders , Olofsson Bagge Roger , Nilsson Jonas A. , Ny Lars
Published: May 2023

Figure S5. Immunohistochemistry magenta of a) Immunohistochemistry magenta showing CCL21 and corresponding CCR7 expression within tumor biopsies for patient 1-013 and 3-009. b) Patient 1-013, a lymph node biopsy showing CD20, CD3 and CCL21 staining with serial sections. c) Patient 2-027, a liver met biopsy showing SOX10, CCR7, TK1 and PDL1 (DAB) within the same areas as shown in Fig 6.
Figure S4 from Chemokine Analysis in Patients with Metastatic Uveal Melanoma Suggests a Role for CCL21 Signaling in Combined Epigenetic Therapy and Checkpoint Immunotherapy
Authors: Sah Vasu R. , Jespersen Henrik , Karlsson Joakim , Nilsson Lisa M. , Bergqvist Mattias , Johansson Iva , Carneiro Ana , Helgadottir Hildur , Levin Max , Ullenhag Gustav , Ståhlberg Anders , Olofsson Bagge Roger , Nilsson Jonas A. , Ny Lars
Published: May 2023

Figure S4. Kaplan–Meier analysis showing PFS of a) CCL13 and b) IL-21. c) Flow cytometry plots showing CCR7 and CD45RA gating among CD3 positive cells in blood samples d) Flow cytometry based comparison between short (n=18) and long (n=6) term survivors with CD3+CCR7+ % (T naive, T stem cell memory and T central memory) analysis using pretreatment blood samples and e) Kaplan–Meier analysis showing Overall survival with median CD3+CCR7+ % (n=24).
Figure S3 from Chemokine Analysis in Patients with Metastatic Uveal Melanoma Suggests a Role for CCL21 Signaling in Combined Epigenetic Therapy and Checkpoint Immunotherapy
Authors: Sah Vasu R. , Jespersen Henrik , Karlsson Joakim , Nilsson Lisa M. , Bergqvist Mattias , Johansson Iva , Carneiro Ana , Helgadottir Hildur , Levin Max , Ullenhag Gustav , Ståhlberg Anders , Olofsson Bagge Roger , Nilsson Jonas A. , Ny Lars
Published: May 2023

Figure S3. a) Heat map showing plasma chemokine and cytokine levels pre-treatment to end of study (EOS) in patients with progressive disease (PD) b) Heat map of plasma chemokine levels pre-treatment to EOS in stable disease (SD) patients. c) Heat map of plasma chemokine levels pre-treatment to EOS in partial response (PR) patients d) Levels of CXCL9 in different response groups pretreatment and EOS. e) Levels of CXCL9 pretreatment and EOS in patients surviving longer or shorter in the PEMDAC trail.
FIGURE 3 from Chemokine Analysis in Patients with Metastatic Uveal Melanoma Suggests a Role for CCL21 Signaling in Combined Epigenetic Therapy and Checkpoint Immunotherapy
Authors: Sah Vasu R. , Jespersen Henrik , Karlsson Joakim , Nilsson Lisa M. , Bergqvist Mattias , Johansson Iva , Carneiro Ana , Helgadottir Hildur , Levin Max , Ullenhag Gustav , Ståhlberg Anders , Olofsson Bagge Roger , Nilsson Jonas A. , Ny Lars
Published: May 2023

A, Heatmap showing TK1 activity value (DuA) from PEMDAC patient plasma samples, grouped within response groups. Each square is a timepoint for each patient and shows TK1 levels from pretreatment to end of study, until otherwise stated. Total plasma samples analyzed for TK1 = 287. B, Longitudinal TK1 activity for individual patients, as shown in A. C and D, Kaplan–Meier analysis showing PFS and OS, respectively for pretreatment TK1 values using a threshold of 150 DuA (median TK1 for all samples = 113). Patients with nonavailability of pretreatment samples were excluded from the analysis. E, IHC of TK1 showing nuclear/cytoplasmic magenta staining in patient biopsies 2-027, 3-012, and 3-010. F, Comparison between pretreatment TK1 values for short- and long-term survivors. G, Correlation between pretreatment TK1 (DuA) and circulating tumor DNA (counts/mL) matched patient samples (n = 21). All statistical tests were unpaired two-tailed t tests, assuming equal variance, with *, P < 0.05; **, P < 0.01; ***, P < 0.001.
Data from Deconstructing Olfactory Epithelium Developmental Pathways in Olfactory Neuroblastoma
Authors: Finlay John B. , Abi Hachem Ralph , Jang David W. , Osazuwa-Peters Nosayaba , Goldstein Bradley J.
Published: May 2023

Olfactory neuroblastoma is a rare tumor arising from the olfactory cleft region of the nasal cavity. Because of the low incidence of this tumor, as well as an absence of established cell lines and murine models, understanding the mechanisms driving olfactory neuroblastoma pathobiology has been challenging. Here, we sought to apply advances from research on the human olfactory epithelial neurogenic niche, along with new biocomputational approaches, to better understand the cellular and molecular factors in low- and high-grade olfactory neuroblastoma and how specific transcriptomic markers may predict prognosis. We analyzed a total of 19 olfactory neuroblastoma samples with available bulk RNA-sequencing and survival data, along with 10 samples from normal olfactory epithelium. A bulk RNA-sequencing deconvolution model identified a significant increase in globose basal cell (GBC) and CD8 T-cell identities in high-grade tumors (GBC from ∼0% to 8%, CD8 T cell from 0.7% to 2.2%), and significant decreases in mature neuronal, Bowman's gland, and olfactory ensheathing programs, in high-grade tumors (mature neuronal from 3.7% to ∼0%, Bowman's gland from 18.6% to 10.5%, olfactory ensheathing from 3.4% to 1.1%). Trajectory analysis identified potential regulatory pathways in proliferative olfactory neuroblastoma cells, including PRC2, which was validated by immunofluorescence staining. Survival analysis guided by gene expression in bulk RNA-sequencing data identified favorable prognostic markers such as SOX9, S100B, and PLP1 expression.
Significance:
Our analyses provide a basis for additional research on olfactory neuroblastoma management, as well as identification of potential new prognostic markers.
Mário de Andrade Entra em Cena
Authors: Fonseca Maria Augusta
Published: May 2023

Este artigo tem como objetivo a trajetória intelectual e artística de Mário de Andrade, tendo por base alguns de seus mais relevantes estudiosos, buscando situar o escritor no período que antecede a Semana de Arte Moderna, entre 1917 e 1921. É no final desse período que Mário escreve a obra inaugural do movimento, Pauliceia desvairada
Zigue-zague Ideológico
Authors: Ferro Tiago
Published: May 2023

Com foco na produção do crítico literário Astrojildo Pereira, este ensaio procura sugerir pontos de contato entre o tempo contraditório da inserção do país na história do capital e a formação de um partido comunista no Brasil.
Mínima Maximaliana
Authors: Antelo Raul
Published: May 2023

A Revolução Russa de 1917 produziu um discurso de energias renovadas em muitos países da América latina. Uma palestra do sociólogo argentino José Ingenieros imediatamente traduzida pela Revista do Brasil ajuda-nos a compreender o debate brasileiro sobre a ideia de uma sociedade em busca de uma nova identidade coletiva, ou seja, uma revolução proletária mundial.
Arruaceiros!
Authors: Eleutério Maria de Lourdes
Published: May 2023

Este ensaio aborda a cidade de São Paulo nas primeiras duas décadas do século XX, quando greves operárias se sucederam entre 1906 e 1920, se intensificando em luta incessante, reivindicando condições dignas de trabalho e moradia. O período é de grande transformação do espaço urbano, simultaneamente marcado por estruturas precárias nos bairros operários e de regiões privilegiadas projetadas para a elite
O Pirralho e o Pré-Modernismo
Authors: Rio Doce Cláudia
Published: May 2023

O artigo apresenta a revista O Pirralho (1911-1918), fundada e dirigida por Oswald de Andrade, buscando explorar, principalmente, aspectos que dialogam com as obras posteriores do autor ou que sejam perceptíveis nas publicações modernistas, anos mais tarde.
“Eclipse da palavra”: travessias no “Canto de Ulisses” de Primo Levi
Authors: Carminati Helena Bressan , Peterle Patricia
Published: May 2023

“Hoje é Primo quem vai comigo buscar a sopa” (LEVI, 1988, p. 163). A frase, aparentemente simples, simboliza o convite para uma troca pouco possível. O espaço concentracionário, onde a linguagem sofre aquilo que, sabiamente, o escritor italiano Primo Levi (1919-1987) define como “eclipse da palavra”, abre-se nesse momento, mais especificamente no capítulo “O canto de Ulisses” de É isto um homem? (1947), para uma outra possibilidade. A palavra que pouco a pouco vai perdendo vida encontra no diálogo entre os dois personagens, Primo Levi e Pikolo, um reencontro com suas subjetividades e não só. É pensando nesse capítulo, e sobretudo, no gesto tradutório como um ato de amorosidade, que este ensaio se inscreve. Nesse sentido, enquanto encontro, a tradução será parte de movimentos que não necessariamente precisam ser binários, isto é, uma cisão entre um dentro e um fora, entre o eu e o outro. De fato, em Tradução como cultura, Gayatri Spivak se refere à tradução como um ato não apenas de leitura íntima, mas também como ato amoroso, de entrega e solidariedade. É a escuta de Pikolo que torna possível o ato tradutório de Levi que recita os versos da Divina Comédia. E se “Nenhuma fala é fala enquanto não é ouvida” (SPIVAK, 2015, p. 58), pretende-se pensar de que forma a tradução acontece em “O canto de Ulisses” e quais caminhos ela torna possíveis dentro de um ambiente tão cerceado quanto o Campo.
A escuta silenciosa da língua de neve que ressoa em “Argéman”– acenos, estrelas por vir
Authors: Santi Elena , Xavier Júlia Bellei
Published: May 2023

Este artigo tem por objetivo evidenciar não somente as características do poema “Argéman”, composto pelo poeta suíço de língua italiana Fabio Pusterla, mas, também, o produto dialógico correspondente à sua tradução pela poeta Prisca Agustoni. Nesse sentido, visamos investigar não só a tarefa heurística desempenhada pelo autor, em sua busca incessante pelos resquícios vitais intrínsecos a diferentes eras e formas de ser, mas, também, o trabalho desenvolvido pela tradutora, que, perscrutando as palavras em sua esfera de partida, procura sondar suas reverberações na esfera de chegada. Assim sendo, tencionamos enfatizar os caminhos pelos quais Agustoni enveredou a fim de “recomeçar a luta da escritura para transformá-la novamente em dança” (PERRONE-MOISÉS, 2013, p. 32), caminhos esses que apostam na escuta dos fragmentos de natureza evocados pelo poema em análise, bem como na irrefutável sensibilidade que o atravessa. Dessa maneira, pretendemos salientar também os efeitos gerados pelo encontro de díspares lentes poéticas e linguageiras, ecoando os silêncios, as entregas e os inúmeros atos de fé àquelas inerentes.
Il libro tradotto come libro-corpo
Authors: Rondini Alessandra
Published: May 2023

Il testo si presenta come un corpo, che viene plasmato dall’ambiente culturale di cui è espressione e che porta su di sé l’impronta di chi lo genera. La traduzione è un viaggio, tra mondi, esperienze di significato, interpretazioni, diversi. Nel momento in cui vive questa esperienza, una sorta di migrazione verso un altro universo di significato, il libro-corpo si trova a dover vivere un processo di inclusione, in un nuovo mondo, che può anche non accettarlo per quello che è e segnarlo, cercare di renderlo più simile a ciò che è conosciuto e, quindi, rassicurante. Interventi sul libro-corpo paragonabili a certi segni rituali, che testimoniano un passaggio, tatuaggi che possono aggiungere un qualcosa di interessante, ma che talvolta sono vere e proprie cicatrici, che lo feriscono profondamente. Si potrebbe parlare di manipolazione del libro-corpo, di cui i vari paratesti fanno parte a pieno titolo, di riletture e riscritture che alcune volte possono portare a esiti soddisfacenti, altre deludenti. I romanzi degli anni Trenta di Jorge Amado, Graciliano Ramos e José Lins do Rego sono passati attraverso questo rito di passaggio nel momento in cui sono stati tradotti in italiano. Alcuni segni sono più nascosti e risulta più difficile individuarli a prima vista, può essere necessario un rapporto di maggiore intimità tra l’osservatore e il corpo che ne è portatore; altri invece possono essere colti a un primo sguardo.
"Oito escritores" de Michele Mari: dos fios da imaginação à agulha da sintaxe
Authors: Gonçalves Victor , Santurbano Andrea
Published: May 2023

O presente artigo pretende apresentar características singulares da literatura do autor milanês Michele Mari através do conto traduzido ao português Oito escritores (2019 [1997]), incluso na coletânea de contos Tu, sanguinosa infanzia (2009), ainda sem tradução. Mari é um autor que ao mesmo tempo que dialoga com algumas ideias da pós-modernidade, dela se afasta, recorrendo aos clássicos e estilos literários do Settecento e Ottocento, por exemplo. Sua obra reflete o experimentalismo das formas literárias, passeando pelo gótico, fantástico e pelos livros de aventura. Em Mari, a ideia de escrita está ligada à infância e suas perdas, ao mundo privado, mas também coletivo, ao fragmento (episódico) mas também às narrativas longas. Otto scrittori é um conto que contém essas características. Há nele um trabalho intenso de linguagem e intertextualidade, recuperando obras e autores como Poe, Stevenson e Salgari, para citar três de oito. O objetivo do artigo é ler e analisar os elementos literários que constituem a narrativa marinheiresca e fantástica do conto Otto scrittori, evidenciando, simultaneamente, aspectos linguísticos característicos da escrita de Mari que o filólogo Luca Serianni (2021) chamou de “hiperliterários”.
Facultative male investment in prolonged mate-guarding in a butterfly
Authors: Gór Ádám , Fónagy Adrien , Pásztor Kata , Szigeti Viktor , Lang Zsolt , Kis János
Published: May 2023

Sexual conflict over mating may induce male mate-guarding prolonged in time in the absence of males, via devices such as mating plugs, widespread in insects. In most Lepidoptera, internal plugs are common, while in some butterfly families large external devices, called sphragides, evolved independently. Lack of, or incomplete sphragis in a few individuals were reported in sphragis-bearing species. Previous studies focused on typifying species-specific devices in a few specimens of many species. In contrast, we investigated alternative mate-guarding devices in detail of a sphragis-bearing butterfly. We conducted a six-year observational field study in a Clouded Apollo Parnassius mnemosyne population. Inspecting 492 females, we identified 3 different devices, filament, stopple and shield (i.e., sphragis) increasing in size and structure complexity, implying differential male investment and effectiveness in securing paternity. Shield dimensions, colour and all devices’ shapes varied considerably. Shields were far more frequent than other devices. Some devices were lost, and a few were video-recorded when removed by males, showing the role of different parts of male external genitalia. We discuss potential causes of device variation and the role of removal attempts, and assess potential costs and benefits for both sexes.
Music across Borders in the Ancient Mediterranean World
Authors: Casali Giovanna , Zangrando Alessia
Published: May 2023

This report provides a conspectus of the round table organised by MOISA for the 21st IMS Quinquennial Congress (IMS 2022). In line with the theme of the congress, namely Music across Borders, the round table focused on the concept of ‘musical mobility’ in the Ancient Mediterranean World, starting from the assumption that the musical identity of the ancient Mediterranean peoples was significantly determined by the mobility of music, concerning dance as well as musicians and musical instruments. The papers presented touched on these different fields, showing how musicology needs to continue a dialogue with new methodologies and approaches of investigation to study and interpret ancient music, including an anthropological perspective.
Doppler Lidar Measurements of Wind Variability and LLJ Properties in Central Oklahoma during the August 2017 Land–Atmosphere Feedback Experiment
Authors: Pichugina Yelena L. , Banta Robert M. , Brewer W. Alan , Turner D. D. , Wulfmeyer V. O. , Strobach E. J. , Baidar S. , Carroll B. J.
Published: May 2023

Low-level jets (LLJs) are an important nocturnal source of wind energy in the U.S. Great Plains. An August 2017 lidar-based field-measurement campaign [the Land–Atmosphere Feedback Experiment (LAFE)] studied LLJs over the central SGP site in Oklahoma and found nearly equal occurrences of the usual southerly jets and postfrontal northeasterly jets—typically rare during this season—for an opportunity to compare the two types of LLJs during this month. Southerly winds were stronger than the northeasterlies by more than 4 m s−1 on average, reflecting a significantly higher frequency of winds stronger than 12 m s−1. The analysis of this dataset has been expanded to other SGP Doppler-lidar sites to quantify the variability of winds and LLJ properties between sites of different land use. Geographic variations of winds over the study area were noted: on southerly wind nights, the winds blew stronger at the highest, westernmost sites by 2 m s−1, whereas on the northeasterly flow nights, the easternmost sites had the strongest wind speeds. Lidar measurements at 5 sites during August 2017, contrasted to the 2016–21 summertime data, revealed unusual wind and LLJ conditions. Temporal hodographs using hourly averaged winds at multiple heights revealed unorganized behavior in the turbulent stable boundary layer (SBL) below the jet nose. Above the nose, some nights showed veering qualitatively similar to inertial oscillation (IO) behavior, but at amplitudes much smaller than expected for an IO, whereas other nights showed little veering. Vertical hodographs had a linear shape in the SBL, indicating little directional shear there, and veering above, resulting in a hook-shaped hodograph with height.

Significance Statement

Doppler-lidar measurements at the Atmospheric Radiation Measurement (ARM) Southern Great Plains (SGP) were used to quantify the variability of winds and low-level jet (LLJ) properties between five sites of different land use and wind regimes across this area. Knowledge of wind and LLJ structure and dynamics is important for many applications, and strong southerly LLJ winds at night are an important resource for wind energy. The analysis of multiyear (2016–21) summertime LLJ parameters provided insight into the LLJ climatology in this part of the Great Plains.
An Approach for Assessing Misclassification of Tornado Characteristics Using Damage
Authors: Lombardo Franklin T. , Wienhoff Zachary B. , Rhee Daniel M. , Nevill Justin B. , Poole Charlotte A.
Published: May 2023

Tornado characteristics (e.g., frequency and intensity) are challenging to capture. Assessment of tornado characteristics typically requires damage as a proxy. The lack of validation in the enhanced Fujita (EF) scale and the likelihood of rural tornadoes suggests that tornado characteristics are not accurately captured. This paper presents an approach to quantify the potential misclassification of tornado characteristics using Monte Carlo simulation for residential structures in rural areas. An analytical tornado wind field model coupled with fragility curves generates degrees of damage (i.e., DOD) from the EF scale in a wind speed–to-damage approach. The simulated DODs are then used to derive damage-to–wind speed relationships built from the National Weather Service Damage Assessment Toolkit (NWS DAT). Comparisons are then made between the simulated tornado characteristics and those derived from damage. Results from the simulations show a substantial proportion of tornadoes were “missed” and path width and pathlength on average are underestimated. An EF4 rating based on damage is favored for EF3–EF5 simulated tornadoes. A linear regression was utilized and determined damage-based wind speeds of different percentiles, damage length, damage width, and the number of structures rated at a particular DOD were important for prediction. The distribution of DODs was also used to predict wind speed and the associated intensity rating. These methods were tested on actual tornado cases. Tornadoes that have the same damage-based peak wind speed can be objectively assessed to determine differences in overall intensity. The results also raise questions about the level of confidence when assessing wind speed based on damage.