bluuebunny/crossref_metadata_2025 huggingface dataset:
A stoic and altruistic orientation towards their work: a qualitative study of healthcare professionals’ experiences of awaiting a COVID-19 test result
Authors: Missel Malene , Bernild Camilla , Dagyaran Ilkay , Christensen Signe Westh , Berg Selina Kikkenborg
Published: November 2020

Background

Extensive measures to reduce person-to-person transmission of COVID-19 are required to control the current outbreak. Special attention is directed at healthcare professionals as reducing the risk of infection in healthcare is essential. The purpose of this study was to explore healthcare professionals’ experiences of awaiting a test result for a potential COVID-19 infection.

Methods

Qualitative interviews with 15 healthcare professionals were performed, underpinned by a phenomenological hermeneutical analytical framework.

Results

The participating healthcare professionals’ experiences of awaiting a COVID-19 test result were found to be associated with a stoic and altruistic orientation towards their work. These healthcare professionals presented a strong professional identity overriding most concerns about their own health. The result of the coronavirus test was a decisive parameter for whether healthcare professionals could return to work. The healthcare professionals were aware that their family and friends were having a hard time knowing that the COVID-19 infection risk was part of their jobs. This concern did not, however, cause the healthcare professionals to falter in their belief that they were doing the right thing by focusing on their core area. The threat to own health ran through the minds of the healthcare professionals occasionally, which makes access to testing particularly important.

Conclusion

The participating healthcare professionals had a strong professional identity. However, a discrepancy between an altruistic role as a healthcare professional and the expectations that come from the community was illuminated. A mental health coronavirus hotline for healthcare professionals is suggested.
Bioavailability of subcutaneous and intramuscular administrated buprenorphine in New Zealand White rabbits
Authors: Askar Raad , Fredriksson Elin , Manell Elin , Hedeland Mikael , Bondesson Ulf , Bate Simon , Olsén Lena , Hedenqvist Patricia
Published: November 2020

Background

Buprenorphine is one of the most used analgesics for postoperative pain in rabbits. The recommended dose in rabbits (0.01–0.05 mg/kg) is the same for intravenous (IV), intramuscular (IM), and subcutaneous (SC) administration, despite lack of pharmacokinetic data. Five male and five female New Zealand White rabbits (mean ± SD body weight 3.1 ± 0.3 kg) were administered 0.05 mg/kg buprenorphine by the IV, IM and SC routes and 0.1 mg/kg by the SC route, in a cross-over design with two-week wash-out periods between treatments. Blood was collected before, and up to 8 h post buprenorphine injection, for determination of serum levels by UPHLC-MS/MS.

Results

The area under the time concentration curve (AUC0-t) was lower after SC (398 ± 155 ng/mL/min) than IM (696 ± 168 ng/mL/min, p < 0.001) and IV (789 ± 189 ng/mL/min, p < 0.001) administration. The maximum serum concentration was lower after SC (2.2 ± 1.4 ng/mL) than after IM (11 ± 3.2 ng/mL) administration (p < 0.001). The bioavailability was lower after SC (50 ± 19%) than after IM (95 ± 21%) administration (p = 0.006). The elimination half-life was longer after SC (260 ± 120 min) than after IM (148 ± 26 min, p = 0.002) as well as IV (139 ± 33 min) injection (p < 0.001). An increase in the SC dose from 0.05 to 0.1 mg/kg resulted in an increase in the area under the time concentration curve of 50% in female (p = 0.022) and 165% in male rabbits (p < 0.001). The bioavailability did not change in the females (36 ± 14%, p = 0.6), whereas it increased in the males (71 ± 23%, p = 0.008).

Conclusions

The lower bioavailability of 0.05 mg/kg buprenorphine after SC administration could explain the lack of efficacy seen in clinical pain studies in rabbits, using this route. For immediate pain relief, IV or IM administration is therefore be recommended, whereas SC administration may be useful to sustain analgesic serum levels, once efficient pain relief has been achieved. The current data do not support an increase in dose to compensate for the lower SC bioavailability.
A cross sectional study of unmet need for health services amongst urban refugees and asylum seekers in Thailand in comparison with Thai population, 2019
Authors: Suphanchaimat Rapeepong , Sinam Pigunkaew , Phaiyarom Mathudara , Pudpong Nareerut , Julchoo Sataporn , Kunpeuk Watinee , Thammawijaya Panithee
Published: November 2020

Background

Although the Thai government has introduced policies to promote the health of migrants, it is still the case that urban refugees and asylum seekers (URAS) seem to be neglected. This study aimed to explore the degree of healthcare access through the perspective of unmet need in URAS, relative to the Thai population.

Methods

A cross-sectional survey, using a self-reporting questionnaire adapted from the Thai Health and Welfare Survey (HWS), was performed in late 2019, with 181 URAS completing the survey. The respondents were were randomly selected from the roster of the Bangkok Refugee Center. The data of the URAS survey were combined with data of the Thai population (n = 2941) from the HWS. Unmet need for health services was defined as the status of needing healthcare in the past 12 months but failing to receive it. Bivariate analysis was conducted to explore the demographic and unmet need difference between URAS and Thais. Multivariable logistic regression and mixed-effects (ME) model were performed to determine factors associated with unmet need.

Results

Overall, URAS were young, less educated and living in more economically deprived households, compared with Thais. About 98% of URAS were uninsured by any of the existing health insurance schemes. The prevalence of unmet need among URAS was significantly higher than among Thais in both outpatient (OP) and inpatient (IP) services (54.1% versus 2.1 and 28.0% versus 2.1%, respectively). Being uninsured showed the strongest association with unmet need, especially for OP care. The association between insurance status and unmet need was more pronounced in the ME model, relative to multivariable logistic regression. URAS migrating from Arab nations suffered from unmet need to a greater extent, compared with those originating from non-Arab nations.

Conclusion

The prevalence of unmet need in URAS was drastically high, relative to the prevalence in Thais. Factors correlated with unmet need included advanced age, lower educational achievement, and, most evidently, being uninsured. Policy makers should consider a policy option to enrol URAS in the nationwide public insurance scheme to create health security for Thai society.
Development and validation of the Short Professional Quality of Life Scale based on versions IV and V of the Professional Quality of Life Scale
Authors: Galiana Laura , Oliver Amparo , Arena Fernanda , De Simone Gustavo , Tomás José M. , Vidal-Blanco Gabriel , Muñoz-Martínez Inmaculada , Sansó Noemí
Published: November 2020

Background

This research presents a short version of the Professional Quality of Life (ProQOL) scale, one of the most frequently used questionnaires in the arena of applied healthcare investigation. It measures burnout (BO), compassion fatigue (CF), and compassion satisfaction (CS).

Methods

A 9-item version of the ProQOL was developed. In Study 1, this short version, which used items from version IV of the ProQOL, was administered to 817 palliative care professionals from Spain, Argentina, and Brazil. In Study 2, the same nine items, but this time from version V of the ProQOL, were administered to 296 Spanish palliative care professionals.

Results

Study 1: The Short ProQOL showed an adequate internal structure, and invariance across the countries studied (χ2(106) = 185.620 (p < 0.001), CFI = .929, RMSEA = 0.058 [0.044, 0.072], SRMR = 0.081). Argentinians showed higher levels of BO (mean difference = 0.172, p = 0.042, Cohen’s d = 0.168), whereas Brazilians showed higher levels of CF (Mean difference = 0.384, p = 0.002, Cohen’s d = 0.352). Study 2: the Short ProQOL again showed adequate internal structure and reliability (χ2(24) = 134.504 (p < 0.001); CFI = 0.953; RMSEA = 0.126 [0.106, 0.147]; SRMR = 0.063), and was related to coping with death, self-compassion, and self-care.

Conclusions

The Short ProQOL could help facilitate the application of harmonizing measurements and its use for cross-cultural comparisons and occupational health monitoring was satisfactory.
Chimeric antigen receptor T-cell therapy in glioblastoma: charging the T cells to fight
Authors: Land Craig A. , Musich Phillip R. , Haydar Dalia , Krenciute Giedre , Xie Qian
Published: November 2020

Glioblastoma multiforme (GBM) is the most common malignant brain cancer that invades normal brain tissue and impedes surgical eradication, resulting in early local recurrence and high mortality. In addition, most therapeutic agents lack permeability across the blood brain barrier (BBB), further reducing the efficacy of chemotherapy. Thus, effective treatment against GBM requires tumor specific targets and efficient intracranial drug delivery. With the most recent advances in immunotherapy, genetically engineered T cells with chimeric antigen receptors (CARs) are becoming a promising approach for treating cancer. By transducing T lymphocytes with CAR constructs containing a tumor-associated antigen (TAA) recognition domain linked to the constant regions of a signaling T cell receptor, CAR T cells may recognize a predefined TAA with high specificity in a non-MHC restricted manner, and is independent of antigen processing. Active T cells can travel across the BBB, providing additional advantage for drug delivery and tumor targeting. Here we review the CAR design and technical innovations, the major targets that are in pre-clinical and clinical development with a focus on GBM, and multiple strategies developed to improve CAR T cell efficacy.
Safety and immunogenicity of the quadrivalent human papillomavirus vaccine in patients with juvenile dermatomyositis: a real-world multicentre study
Authors: Grein Ingrid Herta Rotstein , Pinto Natalia Balera Ferreira , Groot Noortje , Martins Camila Bertini , Lobo Aline , Aikawa Nadia Emi , Barbosa Cassia , Terreri Maria Teresa , da Fraga Aline Coelho Moreira , de Oliveira Sheila Knupp Feitosa , Sztajnbok Flavio , Paim Marques Luciana B. , Islabão Aline Garcia , Appenzeller Simone , Bica Blanca , de Oliveira Sato Juliana , Magalhães Claudia Saad , Ferriani Virgínia , Pasmans Hella , Schepp Rutger , van der Klis Fiona , de Roock Sytze , Wulffraat Nico , Pileggi Gecilmara Salviato
Published: November 2020

Background

Concerns about the safety and efficacy of vaccines in patients with autoimmune diseases (AID) have led to contradictions and low vaccination coverage in this population, who are at a higher risk of infections, including by human papillomavirus (HPV). Although HPV vaccines have been recommended for immunocompromised patients, there is still a lack of data to support its use for AID patients, such as juvenile dermatomyositis (JDM) patients. The aim of this study was to assess the safety and immunogenicity of the quadrivalent HPV (qHPV) vaccine in a cohort of JDM patients.

Methods

JDM patients aged from 9 to 20 years and healthy controls (HC) were enrolled to receive a 3-dose schedule of qHPV vaccine from March/2014 to March/2016. Study visits were performed before the first dose, 1 month after the second and third doses, and 6 months after the third dose. Participants completed a diary of possible adverse events for 14 days following each dose of vaccination (AEFV). Disease activity and current therapy were analyzed at each visit for JDM patients. In addition, serum samples from all participants were collected to test antibody concentrations against HPV16 and 18 at each visit. Participant recruitment was conducted in ten Brazilian centres. From 47 eligible JDM patients and 41 HC, 42 and 35, respectively, completed the 3-dose schedule of the vaccine, given that five JDM patients and two HC had received doses prior to their inclusion in the study.

Results

The AEFVs presented by the participants were mild and in general did not differ between JDM and HC groups. No severe AEFVs were related to the vaccination. Disease activity was stable, or even improved during the follow-up. One month after the third dose of the vaccine the JDM group presented seropositivity of 100% for HPV16 and 97% for HPV18, similarly to the HC group, who presented 100% for both serotypes (p = 1.000). Six months after the third dose the seropositivity for the patient group was 94% for both HPV types.

Conclusions

The HPV vaccination in this cohort of JDM patients was safe and immunogenic. Since the seropositivity against HPV16 and 18 was very high after the 3-dose schedule, this regimen should be recommended for JDM patients.

Trial registration

Brazilian Clinical Trials Registry, number: RBR-9ypbtf. Registered 20 March 2018 – Retrospectively registered.
The global governance of antimicrobial resistance: a cross-country study of alignment between the global action plan and national action plans
Authors: Munkholm Louise , Rubin Olivier
Published: November 2020

Background

Antimicrobial resistance (AMR) is a growing problem worldwide in need of global coordinated action. With the endorsement of the Global Action Plan (GAP) on AMR in 2015, the 194 member states of the World Health Organization committed to integrating the five objectives and corresponding actions of the GAP into national action plans (NAPs) on AMR. The article analyzes patterns of alignment between existing NAPs and the GAP, bringing to the fore new methodologies for exploring the relationship between globally driven health policies and activities at the national level, taking income, geography and governance factors into account.

Methods

The article investigates the global governance of AMR. Concretely, two proxies are devised to measure vertical and horizontal alignment between the GAP and existing NAPs: (i) a syntactic indicator measuring the degree of verbatim overlap between the GAP and the NAPs; and (ii) a content indicator measuring the extent to which the objectives and corresponding actions outlined in the GAP are addressed in the NAPs. Vertical alignment is measured by the extent to which each NAP overlaps with the GAP. Horizontal alignment is explored by measuring the degree to which NAPs overlap with other NAPs across regions and income groups. In addition, NAP implementation is explored using the Global Database for Antimicrobial Resistance Country Self-Assessment.

Findings

We find strong evidence of vertical alignment, particularly among low-income countries and lower-middle-income countries but weaker evidence of horizontal alignment within regions. In general, we find the NAPs in our sample to be mostly aligned with the GAP’s five overarching objectives while only moderately aligned with the recommended corresponding actions. Furthermore, we see several cases of what can be termed ‘isomorphic mimicry’, characterized by strong alignment in the policies outlined but much lower levels of alignment in terms of actual implemented policies.

Conclusion

To strengthen the alignment of national AMR policies, we recommend global governance initiatives based on individualized responsibilities some of which should be legally binding. Our study provides limited evidence of horizontal alignment within regions, which implies that regional governance institutions (e.g., WHO regional offices) should primarily act as mediators between global and local demands to strengthen a global governance regime that minimizes policy fragmentation and mimicry behavior across member states.
Degradation of subchondral bone collagen in the weight-bearing area of femoral head is associated with osteoarthritis and osteonecrosis
Authors: Wu Zongyi , Wang Bingzhang , Tang Jiahao , Bai Bingli , Weng Sheji , Xie Zhongjie , Shen Zijian , Yan Deyi , Chen Liang , Zhang Jingdong , Yang Lei
Published: November 2020

Background

The aim of the study was to evaluate the change of subchondral bone collagen and trabecular bone in the weight-bearing area of femoral head from patients with osteoarthritis (OA) or osteonecrosis of femoral head (ONFH), and discuss the effect of collagen degradation on OA and ONFH.

Methods

Femoral heads from patients with femoral neck fracture (FNF) were collected as control group. All collected samples were divided into OA group (N = 10), ONFH group (N = 10), and FNF group (N = 10). Differences of subchondral bone collagen were compared through scanning electron microscope (SEM) observation, immunohistochemistry staining, and Masson’s trichrome staining. Alteration of subchondral bone was displayed through hematoxylin and eosin (H&E) staining and gross morphology.

Results

SEM results showed that collagen fibers in OA and ONFH group appeared to be thinner, rougher, sparser, and more wizened. Immunohistochemistry and Masson’s trichrome staining results demonstrated that the content of collagen fibers in the OA and ONFH group was obviously less than the FNF group. H&E staining results showed that trabecular bone in OA and ONFH group appeared to be thinner and ruptured. Gross morphology results showed that the degeneration and destruction of cartilage and subchondral bone in OA and ONFH group were severer than FNF group. The characteristics mentioned above in ONFH group were more apparent than OA group.

Conclusions

This study revealed that degradation of collagen fibers from subchondral bone in the weight-bearing area of femoral head was associated with OA and ONFH, which may help to find new therapeutic strategies of the diseases.
Umbilical cord-derived Wharton’s jelly for regenerative medicine applications in orthopedic surgery: a systematic review protocol
Authors: Main Benjamin J. , Valk Josiah A. , Maffulli Nicola , Rodriguez Hugo C. , Gupta Manu , Stone Ian W. , El-Amin Saadiq F. , Gupta Ashim
Published: November 2020

Background

Musculoskeletal injuries and conditions affect millions of individuals. These ailments are typically managed by immobilization, physiotherapy, or activity modification. Regenerative medicine has experienced tremendous growth in the past decades, especially in musculoskeletal medicine. Umbilical cord-derived Wharton’s jelly is an exciting new option for such therapies. Wharton’s jelly is a connective tissue located within the umbilical cord largely composed of mesenchymal stem cells and extracellular matrix components, including collagen, chondroitin sulfate, hyaluronic acid, and sulfated proteoglycans. Wharton’s jelly is a promising and applicable biologic source for orthopedic regenerative application.

Methods

A systematic search will be conducted in PubMed, ScienceDirect, and Google Scholar databases of English, Italian, French, Spanish, and Portuguese language articles published to date. References will be screened and assessed for eligibility by two independent reviewers as per PRISMA guidelines. Articles will be considered without exclusion to sex, activity, or age. Studies will be included if they used culture-expanded, mesenchymal stem/stromal cells of mesenchymal stem cells and/or connective tissue obtained from Wharton’s jelly. Studies will be excluded if Wharton’s jelly is not the sole experimental examined cell type. Placebos, conventional non-operative therapies including steroid injections, exercise, and NSAIDs will be compared. The study selection process will be performed independently by two reviewers using a reference software. Data synthesis and meta-analysis will be performed separately for clinical and pre-clinical studies.

Discussion

The results will be published in relevant peer-reviewed scientific journals. Investigators will present results at national or international conferences.

Trial registration

The protocol was registered on PROSPERO international prospective register of systematic reviews prior to commencement, CRD42020182487.
ARTP/EMS-combined multiple mutagenesis efficiently improved production of raw starch-degrading enzymes in Penicillium oxalicum and characterization of the enzyme-hyperproducing mutant
Authors: Gu Li-Sha , Tan Ming-Zhu , Li Shi-Huan , Zhang Ting , Zhang Qi-Qiang , Li Cheng-Xi , Luo Xue-Mei , Feng Jia-Xun , Zhao Shuai
Published: November 2020

Background

Application of raw starch-degrading enzymes (RSDEs) in starch processing for biofuel production can effectively reduce energy consumption and processing costs. RSDEs are generally produced by filamentous fungi, such as Penicillium oxalicum, but with very low yields, which seriously hampers industrialization of raw starch processing. Breeding assisted by random mutagenesis is an efficient way to improve fungal enzyme production.

Results

A total of 3532 P. oxalicum colonies were generated after multiple rounds of mutagenesis, by atmospheric and room-temperature plasma (ARTP) and/or ethyl methanesulfonate (EMS). Of these, one mutant A2-13 had the highest RSDE activity of 162.7 U/mL, using raw cassava flour as substrate, a yield increase of 61.1%, compared with that of the starting strain, OXPoxGA15A. RSDE activity of A2-13 further increased to 191.0 U/mL, through optimization of culture conditions. Increased expression of major amylase genes, including the raw starch-degrading glucoamylase gene, PoxGA15A, and its regulatory gene, PoxAmyR, as well as several single-nucleotide polymorphisms in the A2-13 genome, were detected by real-time reverse transcription quantitative PCR and genomic re-sequencing, respectively. In addition, crude RSDEs produced by A2-13, combined with commercial α-amylase, could efficiently digest raw corn flour and cassava flour at 40 °C.

Conclusions

Overall, ARTP/EMS-combined mutagenesis effectively improved fungal RSDE yield. An RSDE-hyperproducing mutant, A2-13, was obtained, and its RSDEs could efficiently hydrolyze raw starch, in combination with commercial α-amylase at low temperature, which provides a useful RSDE resource for future starch processing.
Sand flies (Diptera: Psychodidae) in eight Balkan countries: historical review and region-wide entomological survey
Authors: Dvorak Vit , Kasap Ozge Erisoz , Ivovic Vladimir , Mikov Ognyan , Stefanovska Jovana , Martinkovic Franjo , Omeragic Jasmin , Pajovic Igor , Baymak Devrim , Oguz Gizem , Hlavackova Kristyna , Gresova Marketa , Gunay Filiz , Vaselek Slavica , Ayhan Nazli , Lestinova Tereza , Cvetkovikj Aleksandar , Soldo Darinka Klaric , Katerinova Ivelina , Tchakarova Simona , Yılmaz Ayda , Karaoglu Begum , Iranzo Jose Risueno , Kadriaj Perparim , Velo Enkelejda , Ozbel Yusuf , Petric Dusan , Volf Petr , Alten Bulent
Published: November 2020

Background

Sand flies (Diptera: Psychodidae) are medically important vectors of human and veterinary disease-causing agents. Among these, the genus Leishmania (Kinetoplastida: Trypanosomatidae), and phleboviruses are of utmost importance. Despite such significance, updated information about sand fly fauna is missing for Balkan countries where both sand flies and autochtonous leishmaniases are historically present and recently re-emerging. Therefore, a review of historical data on sand fly species composition and distribution in the region was followed by a large-scale entomological survey in eight Balkan countries to provide a recent update on local sand fly fauna.

Methods

The literature search involved the period 1910–2019. The entomological survey was conducted at 1189 sampling stations in eight countries (Bulgaria, Bosnia and Herzegovina, Croatia, Kosovo, Montenegro, North Macedonia, Serbia and Slovenia), covering 49 settlements and 358 sampling sites between June and October in the years 2014 and 2016, accumulating 130 sampling days. We performed a total of 1189 trapping nights at these stations using two types of traps (light and CO2 attraction traps) in each location. Sampling was performed with a minimal duration of 6 (Montenegro) and a maximal of 47 days (Serbia) between 0–1000 m.a.s.l. Collected sand flies were morphologically identified.

Results

In total, 8490 sand fly specimens were collected. Morphological identification showed presence of 14 species belonging to genera Phlebotomus and Sergentomyia. Historical data were critically reviewed and updated with our recent findings. Six species were identified in Bosnia and Herzegovina (2 new records), 5 in Montenegro (2 new records), 5 in Croatia (2 new records), 9 in Bulgaria (5 new records), 11 in North Macedonia (1 new record), 10 in Serbia (no new records), 9 in Kosovo (3 new records) and 4 in Slovenia (no new records).

Conclusions

This study presents results of the first integrated sand fly fauna survey of such scale for the Balkan region, providing first data on sand fly populations for four countries in the study area and presenting new species records for six countries and updated species lists for all surveyed countries. Our findings demonstrate presence of proven and suspected vectors of several Leishmania species.

Graphical Abstract
Spatio-chromatic information available from different neural layers via Gaussianization
Authors: Malo Jesús
Published: November 2020

How much visual information about the retinal images can be extracted from the different layers of the visual pathway?This question depends on the complexity of the visual input, the set of transforms applied to this multivariate input, and the noise of the sensors in the considered layer. Separate subsystems (e.g. opponent channels, spatial filters, nonlinearities of the texture sensors) have been suggested to be organized for optimal information transmission. However, the efficiency of these different layers has not been measured when they operate together on colorimetrically calibrated natural images and using multivariate information-theoretic units over the joint spatio-chromatic array of responses.

In this work, we present a statistical tool to address this question in an appropriate (multivariate) way. Specifically, we propose an empirical estimate of the information transmitted by the system based on a recent Gaussianization technique. The total correlation measured using the proposed estimator is consistent with predictions based on the analytical Jacobian of a standard spatio-chromatic model of the retina–cortex pathway. If the noise at certain representation is proportional to the dynamic range of the response, and one assumes sensors of equivalent noise level, then transmitted information shows the following trends: (1) progressively deeper representations are better in terms of the amount of captured information, (2) the transmitted information up to the cortical representation follows the probability of natural scenes over the chromatic and achromatic dimensions of the stimulus space, (3) the contribution of spatial transforms to capture visual information is substantially greater than the contribution of chromatic transforms, and (4) nonlinearities of the responses contribute substantially to the transmitted information but less than the linear transforms.
Stationary distribution of a stochastic hybrid phytoplankton model with allelopathy
Authors: Ji Weiming , Wang Zhaojuan , Hu Guixin
Published: November 2020

This research proposes and delves into a stochastic competitive phytoplankton model with allelopathy and regime-switching. Sufficient criteria are proffered to ensure that the model possesses a unique ergodic stationary distribution (UESD). Furthermore, it is testified that these criteria are sharp on certain conditions. Some critical functions of regime-switching on the existence of a UESD of the model are disclosed: regime-switching could lead to the appearance of the UESD. The theoretical findings are also applied to research the evolution of Heterocapsa triquetra and Chrysocromulina polylepis.
Successful treatment of severe pneumonia, pyopneumothorax with severe acute respiratory distress syndrome, and septic shock: a case report
Authors: Wang Xi , Lan Jian , Zhang Ruijie , Luo Xiaoqing
Published: November 2020

Background

This article reports a patient who survived severe pneumonia, pyopneumothorax with acute respiratory distress syndrome (ARDS), and septic shock, which is very difficult to treat.

Case presentation

Antibiotics, continuous renal replacement therapy (CRRT), bronchial lavage and other treatments were used to treat a patient with pneumonia, pyopneumothorax, severe ARDS and septic shock. After comprehensive treatment, the patient was successfully treated and survived for a long time.

Conclusions

There is a low successful clinical treatment rate for patients with pneumonia, pyopneumothorax with severe ARDS and septic shock. The successful treatment of this patient benefited from early and effective empirical therapy, targeted drug selection in the later stage, adequate closed thoracic drainage, repeated bronchial lavage, early CRRT, an appropriate respiratory support mode and parameter setting, immunotherapy and nutritional support therapy. This paper proposes a reference diagnosis and treatment solution for similar cases.
High prevalence of Babesia microti in small mammals in Beijing
Authors: Wei Chun-Yan , Wang Xiao-Mei , Wang Zhen-Sheng , Wang Zhi-Hua , Guan Zeng-Zhi , Zhang Lian-Hui , Dou Xiang-Feng , Wang Heng
Published: November 2020

Background

Babesiosis is an emerging tick-borne zoonotic infectious disease. Babesia microti is responsible for most cases of human babesiosis globally. It is important to investigate the prevalence of B. microti in the mammalian host population of a specific region in order to elucidate mechanisms of pathogen transmission and to define geographic areas where humans face the greatest risk of exposure. The aim of this study is to understand the prevalence and genotypes of B. microti in the small mammals that are found in Beijing, China.

Methods

We trapped small mammals from all of the 16 urban, suburban, and outer suburban districts of Beijing during the years 2014, 2017 and 2018. Genomic DNA was extracted from the heart tissues individually and the Babesia 18S rRNA gene was detected by PCR. The genotypes of B. microti were identified based on sequence alignments and phylogenetic analysis. The morphology of the parasites was observed under light microscopy. The risk factors were analyzed statistically based on both univariate analyses and multivariate logistic regression.

Results

A total of 1391 small mammals were collected. Positive infection of B. microti was detected in 12.1% (168/1391) of small mammals from 15 out of the 16 districts. Both Kobe-type and U.S.-type B. microti, accounting for 9.5% and 2.7%, respectively, were identified. Classic diverse morphologic forms of B. microti were observed. Specific types of ecological habitats including shrub areas, broad-leaved forest, and cropland were revealed to be risk factors associated with B. microti infection.

Conclusions

This study demonstrated the wide prevalence of B. microti infection in eight species of small mammals in Beijing, with Kobe-type more prevalent than U.S.-type. This study provides fundamental information for the development of informed prevention and control measures by public health authorities; the data gathered indicates a need for further monitoring of both clinical diseases in individuals presenting with babesiosis-like symptoms, as well as the infection status of ticks in high risk areas.
A robust machine learning approach to SDG data segmentation
Authors: Mwitondi Kassim S. , Munyakazi Isaac , Gatsheni Barnabas N.
Published: November 2020

In the light of the recent technological advances in computing and data explosion, the complex interactions of the Sustainable Development Goals (SDG) present both a challenge and an opportunity to researchers and decision makers across fields and sectors. The deep and wide socio-economic, cultural and technological variations across the globe entail a unified understanding of the SDG project. The complexity of SDGs interactions and the dynamics through their indicators align naturally to technical and application specifics that require interdisciplinary solutions. We present a consilient approach to expounding triggers of SDG indicators. Illustrated through data segmentation, it is designed to unify our understanding of the complex overlap of the SDGs by utilising data from different sources. The paper treats each SDG as a Big Data source node, with the potential to contribute towards a unified understanding of applications across the SDG spectrum. Data for five SDGs was extracted from the United Nations SDG indicators data repository and used to model spatio-temporal variations in search of robust and consilient scientific solutions. Based on a number of pre-determined assumptions on socio-economic and geo-political variations, the data is subjected to sequential analyses, exploring distributional behaviour, component extraction and clustering. All three methods exhibit pronounced variations across samples, with initial distributional and data segmentation patterns isolating South Africa from the remaining five countries. Data randomness is dealt with via a specially developed algorithm for sampling, measuring and assessing, based on repeated samples of different sizes. Results exhibit consistent variations across samples, based on socio-economic, cultural and geo-political variations entailing a unified understanding, across disciplines and sectors. The findings highlight novel paths towards attaining informative patterns for a unified understanding of the triggers of SDG indicators and open new paths to interdisciplinary research.
Pre-harvest field application of enhanced freshness formulation reduces yield loss in orange
Authors: Samwel Jaspa , Msogoya Theodosy , Kudra Abdul , Mtui Hosea Dunstan , Baltazari Anna , Sullivan J. Alan , Subramanian Jayasankar , Mwatawala Maulid Walad
Published: November 2020

Background

Orange (Citrus sinensis L.) production in Tanzania is constrained by several pre-harvest factors that include pests. Hexanal, sprayed as Enhanced Freshness Formulation (EFF) is a relatively new technology that has been reported to reduce pre-harvest loss in fruits. However, the effects of hexanal on pre-harvest yield loss of orange are not known. We studied the effects of hexanal as EFF on yield losses of three sweet orange cultivars namely, Early Valencia, Jaffa, and Late Valencia. Factorial experiments tested the effects of EFF concentration, variety, and time of EFF application on number of dropped fruit, percentage of non-marketable fruit and incidence of pest damage.

Results

Results showed significant negative correlation (p < 0.001) between EFF and the percentage of dropped fruit, non-marketable yield, and incidence of pest damage. An increase in hexanal concentration by 1%, is expected to reduce number of dropped fruit by 50, percentage of non-marketable by 35.6, and incidences of pest damage by 36.5% keeping other factors constant. Results also show significant association (p < 0.001) between time of hexanal application and non-marketable yield. Percentage of dropped fruit is expected to increase by 1 for each day away from harvest, keeping other factors constant.

Conclusion

Pre-harvest application of hexanal as EFF can significantly reduce number of dropped fruits, percentage of non-marketable fruit and incidence of pest damage.
CT-based radiomics features in the prediction of thyroid cartilage invasion from laryngeal and hypopharyngeal squamous cell carcinoma
Authors: Guo Ran , Guo Jian , Zhang Lichen , Qu Xiaoxia , Dai Shuangfeng , Peng Ruchen , Chong Vincent F. H. , Xian Junfang
Published: November 2020

Background

Laryngeal and hypopharyngeal squamous cell carcinoma (LHSCC) with thyroid cartilage invasion are considered T4 and need total laryngectomy. However, the accuracy of preoperative diagnosis of thyroid cartilage invasion remains lower. Therefore, the purpose of this study was to assess the potential of computed tomography (CT)-based radiomics features in the prediction of thyroid cartilage invasion from LHSCC.

Methods

A total of 265 patients with pathologically proven LHSCC were enrolled in this retrospective study (86 with thyroid cartilage invasion and 179 without invasion). Two head and neck radiologists evaluated the thyroid cartilage invasion on CT images. Radiomics features were extracted from venous phase contrast-enhanced CT images. The least absolute shrinkage and selection operator (LASSO) and logistic regression (LR) method were used for dimension reduction and model construction. In addition, the support vector machine-based synthetic minority oversampling (SVMSMOTE) algorithm was adopted to balance the dataset and a new LR-SVMSMOTE model was constructed. The performance of the radiologist and the two models were evaluated with receiver operating characteristic (ROC) curves and compared using the DeLong test.

Results

The areas under the ROC curves (AUCs) in the prediction of thyroid cartilage invasion from LHSCC for the LR-SVMSMOTE model, LR model, and radiologist were 0.905 [95% confidence interval (CI): 0.863 to 0.937)], 0.876 (95%CI: 0.830 to 0.913), and 0.721 (95%CI: 0.663–0.774), respectively. The AUCs of both models were higher than that of the radiologist assessment (all P < 0.001). There was no significant difference in predictive performance between the LR-SVMSMOTE and LR models (P = 0.05).

Conclusions

Models based on CT radiomic features can improve the accuracy of predicting thyroid cartilage invasion from LHSCC and provide a new potentially noninvasive method for preoperative prediction of thyroid cartilage invasion from LHSCC.
Testing spore amyloidity in Agaricales under light microscope: the case study of Tricholoma
Authors: Vizzini Alfredo , Consiglio Giovanni , Setti Ledo
Published: November 2020

Although species of the genusTricholomaare currently considered to produce inamyloid spores, a novel standardized method to test sporal amyloidity (which involves heating the sample in Melzer’s reagent) showed evidence that in the tested species of this genus, which belong in all 10 sections currently recognized from Europe, the spores are amyloid. In two species,T.josserandiiandT. terreum, the spores are also partly dextrinoid. This result provides strong indication that a positive reaction of the spores in Melzer’s reagent could be a character shared by all genera inTricholomataceaes. str.
Typical and atypical CT chest imaging findings of novel coronavirus 19 (COVID-19) in correlation with clinical data: impact on the need to ICU admission, ventilation and mortality
Authors: Emara Doaa M. , Naguib Nagy N. , Moustafa M. A. , Ali Salma M. , El Abd Amr Magdi
Published: November 2020

Background

The aim of this study was to highlight the typical and atypical chest CT imaging features at first presentation in 120 patients who were proved to be COVID-19 by PCR and to correlate these findings with the need for ICU admission, ventilation, and mortality. We retrospectively included 120 patients 71 males (59.2%) and 49 females (40.8%) with a mean age of 47.2 ± 14.4 years. Patients subjected to clinical assessment, CBC, PCR for COVID-19, and non-contrast CT chest at first presentation. Typical and atypical imaging findings were reported and correlated with the clinical findings of the patients, the need for ICU admission, ventilation, and mortality.

Results

Clinically, fever was seen in 112 patients followed by dry cough in 108 patients and malaise in 35 patients. The final outcome was complete recovery in 113 cases and death in 7 cases. Typical CT findings included bilateral peripheral ground-glass opacities (GGO) in 74.7%, multilobar affection in 92.5% while atypical findings such as homogeneous consolidation, pleural effusion, mediastinal lymphadenopathy, and single lobar affection were found in 13.4, 5, 6.7, and 7.5% respectively. A statistically significant association between the presence of white lung, pleural effusion, peripheral GGO, and the need for ICU admission as well as mechanical ventilation was noted. The death was significantly higher among elderly patients; however, no significance was found between the imaging features and mortality.

Conclusion

CT features at first presentation can predict the need for ICU admission and the need for ventilation but cannot predict the mortality outcome of the patients.
Prestack diffraction separation in the common virtual source gather
Authors: Li Chuangjian , Zhao Jingtao , Peng Suping , Cui Xiaoqin
Published: November 2020

Diffractions are the seismic responses of subsurface small-scale geologic discontinuities or inhomogeneities, whose energy is much weaker than that of reflections. To eliminate the masking effect of strong reflections on weak diffractions, a prestack diffraction separation method is proposed based on the common virtual source gather (CVSG) and the median filter. The reflection generated from a reflector can be regarded as originating from a virtual source (or a focused area). According to this behavior, the reflection can be transformed into its corresponding virtual source position to build the CVSG. Unlike the reflection, diffraction cannot be regarded as originating from one virtual source. Therefore, diffractions have a curved shape, whereas reflections are flat in the CVSG. Based on the traveltime moveout method, the reflection traveltime is calculated for describing reflection events and generating CVSG. Theory and a simple synthetic data test demonstrate that there is almost no energy lost when transforming between a shot gather and CVSG. Then, for removing the reflection, the median filter is used in the CVSG where the reflection appears as a linear event. Complex synthetic and field examples demonstrate that the proposed method can flatten reflection events and thus can separate diffraction events from strong reflections in the prestack domain.
Robust estimation of primaries by sparse inversion and Marchenko equation-based workflow for multiple suppression in the case of a shallow water layer and a complex overburden: A 2D case study in the Arabian Gulf
Authors: Staring Myrna , Dukalski Marcin , Belonosov Mikhail , Baardman Rolf H. , Yoo Jewoo , Hegge Rob F. , van Borselen Roald , Wapenaar Kees
Published: November 2020

Suppression of surface-related and internal multiples is an outstanding challenge in seismic data processing. The former is particularly difficult in shallow water, whereas the latter is problematic for targets buried under complex, highly scattering overburdens. We have developed a two-step, amplitude- and phase-preserving, inversion-based workflow that addresses these problems. We apply robust estimation of primaries by sparse inversion (R-EPSI) to solve simultaneously for the surface-related primaries Green’s function and the source wavelet. A significant advantage of the inversion approach of the R-EPSI method is that it does not rely on an adaptive subtraction step that typically limits other demultiple methods such as surface-related multiple elimination. The resulting Green’s function is used as the input to a Marchenko equation-based approach to predict the complex interference pattern of all overburden-generated internal multiples at once. In this approach, no a priori information about the subsurface is needed. In theory, the interbed multiples can be predicted with correct amplitude and phase and, again, no adaptive filters are required. We illustrate this workflow by applying it on an Arabian Gulf field data example. It is crucial that all preprocessing steps are performed in an amplitude-preserving way to restrict any impact on the accuracy of the multiple prediction. In practice, some minor inaccuracies in the processing flow may end up as prediction errors for which corrections will be needed. Hence, we conclude that the use of conservative adaptive filters were necessary to obtain the best results after interbed multiple removal. The obtained results indicate promising suppression of surface-related and interbed multiples.
A correction method based on geometric factor for resistivity log response of thinly laminated sand reservoirs: A case study
Authors: Hu Shuyong , Huang Wenhai , Yao Hengshen , Ling Kegang
Published: November 2020

The log response of thin oil layers is greatly subject to environmental factors such as shoulder beds, resulting in errors high enough to influence the appraisal of rock lithology and fluid properties and increasing the difficulty of interpretation of logging curves and the effective evaluation of thinly laminated sand. The development of high-resolution logging instruments and logging curve processing technology improves the resolution and accuracy of logs, but with some limitations. So far, the geometric factor theory has been an effective approximate approach for induction logging correction. Based on the working principle of the induction/resistivity log and on previous studies, we have developed a new model to correct the resistivity log response of thin layers by taking advantage of the geometric factor. This method can improve the accuracy of the resistivity log for the calculation of porosity and water saturation. Our case study indicates that more reliable resistivity can be acquired to better characterize thin layers.
Rifampin Reduces the Plasma Concentrations of Oral and Intravenous Hydromorphone in Healthy Volunteers
Authors: Lohela Terhi J. , Poikola Satu , Neuvonen Mikko , Niemi Mikko , Backman Janne T. , Olkkola Klaus T. , Lilius Tuomas O.
Published: November 2020

BACKGROUND:

Several opioids are metabolized by the inducible cytochrome P450 (CYP) 3A isozymes. Coadministration with strong inducers of drug metabolism, such as rifampin, can dramatically reduce systemic exposure to these opioids. As the CYP metabolism of hydromorphone is of minor importance, we studied in healthy volunteers whether hydromorphone would be an effective analgesic for patients who concomitantly receive the prototypical enzyme inducer rifampin.

METHODS:

In this paired, randomized, crossover study, 12 participants received oral placebo or rifampin for 8 days. Oral hydromorphone (2.6 mg) was administered on day 6 followed by intravenous hydromorphone (0.02 mg/kg) on day 8. Hydromorphone and hydromorphone-3-glucuronide (HM3G) plasma concentrations were measured for 24 hours and psychomotor responses, including perceived drug effect, change in pupil diameter, and cold pressor threshold were evaluated for 6 hours. Our primary outcome was the change in the area under the concentration–time curve (AUC0–last) of oral and intravenous hydromorphone after pretreatment with rifampin or placebo. Pharmacodynamic parameters and other pharmacokinetic parameters were analyzed as secondary outcomes.

RESULTS:

Rifampin reduced the AUC0–last of oral and intravenous hydromorphone by 43% (ratio to control: 0.57, 90% confidence interval [CI], 0.50-0.65) and 26% (ratio to control: 0.74, 90% CI, 0.69-0.79), respectively. The maximum concentration of oral hydromorphone was reduced by 37% (ratio to control: 0.63, 90% CI, 0.55-0.72), and oral bioavailability decreased from 33% to 26% (ratio to control: 0.78, 90% CI, 0.67-0.91) in the rifampin phase compared with placebo. The HM3G-to-hydromorphone ratio increased by 50% (90% CI, 25-79) and 42% (90% CI, 29-55) after oral and intravenous hydromorphone, respectively. Rifampin did not significantly affect the pharmacodynamic parameters.

CONCLUSIONS:

Rifampin significantly reduces the concentrations of oral and intravenous hydromorphone. This interaction is due to an increase in the first-pass and systemic metabolism of hydromorphone, likely involving induction of uridine 5′-diphospho- glucuronosyltransferase enzymes by rifampin. The enhancement of hydromorphone elimination should be considered when managing pain of patients who are treated with strong enzyme inducers.