bluuebunny/crossref_metadata_2025 huggingface dataset:
UMA ANÁLISE DA TRAJETÓRIA DO PDE-PR NA PERSPECTIVA DE PROFESSORES FORMADORES DA ÁREA DE MATEMÁTICA
Authors: Silva Priscila Gleden Novaes da , Boscarioli Clodis , Vertuan Rodolfo Eduardo
Published: November 2020

O PDE-PR é uma política pública de formação continuada que entre 2007 e 2016 se configurou como uma parceria entre Instituições de Ensino Superior e Educação Básica, tendo mudado de formato após esse período. Apresentamos, neste artigo, as percepções de professores formadores da UNIOESTE, que atuaram na primeira fase do PDE-PR, na área de Matemática, sobre o Programa e os possíveis efeitos dessa participação em sua prática. Um questionário foi enviado para quarenta e nove desses docentes, dos quais obtivemos vinte e nove respondentes. Das análises dasrespostas emergiram suas percepções, apresentando, como principais aspectos positivos a aproximação da Educação Básica ao Ensino Superior; a reflexão de problemas ligados às escolas e ao trabalho do professor; e o afastamento remunerado. Como aspectos negativos foram citados a ascensão exclusiva eobrigatória na carreira por meio do Programa; o desinteresse manifesto em alguns professores; e a seleção dos professores, em geral, já próximos à aposentadoria. Quanto aos efeitos dessa formação na própria prática, foram elencados, como avanço, a aproximação com a realidade escolar e, como desafio, a orientação em áreas diferentes de sua formação e atuação. No formato atual, as principais mudanças dizem respeito ao fato de que não é mais oportunizada uma formação de caráter coletivo, com participação das Universidades e com direito ao professor de afastamento remunerado para a formação, o que exclui pressupostos norteadores confirmados como pontos positivos do Programa.
NARRATIVAS DA FORMAÇÃO E DA ATUAÇÃO DO PEDAGOGO NO PROCESSO DE ENSINO E APRENDIZAGEM DA MATEMÁTICA NOS ANOS INICIAIS DO ENSINO FUNDAMENTAL
Authors: Junior Wagner Alvarenga Vieira , Oliveira Sandra Alves de
Published: November 2020

Este estudo tem como temática central de sua discussão a formação teórico-metodológica do professor que ensina matemática nos anos iniciais do ensino fundamental, o pedagogo. Busca-se analisar os sentidos atribuídos, por estudantes egressas do curso de Pedagogia do Campus XII da Universidade do Estado da Bahia (UNEB), à sua formação matemática e à sua atuação como professoras nos anos iniciais do ensino fundamental; e identificar as contribuições e os desafios da formação matemática nessa atuação. Para tanto, as análises ao longo do texto se respaldam em estudos já realizados nessa vertente, como também em narrativas escritas por estudantes egressas do referido curso. Esta pesquisa de abordagem qualitativa utilizou-se da recolha dos dados dos seguintes instrumentos metodológicos: questionário e narrativas elaboradas pelas seis estudantes egressas da Pedagogia, que atuam como professoras de matemática nos anos iniciais. Os achados da pesquisa alertam para a importância de ser revista a matriz curricular do curso de Pedagogia em relação à formação matemática do pedagogo, pois há apenas um componente curricular com a carga horária de 60 horas/aula que aborda essa questão, o que tem inviabilizado a aprendizagem significativa dossaberes matemáticos. Ademais, o conteúdo, por ser parte imprescindível desse saber, deve ser valorizado e aprofundado não apenas teoricamente, mas também na prática do professor que ensina matemática.
Effects of Long-Term Cover Cropping on Weed Seedbanks
Authors: Nichols Virginia , English Lydia , Carlson Sarah , Gailans Stefan , Liebman Matt
Published: November 2020

Cool-season cover crops have been shown to reduce soil erosion and nutrient discharge from maize (Zea maysL.) and soybean [Glycine max(L.) Merr.] production systems. However, their effects on long-term weed dynamics are not well-understood. We utilized five long-term research trials in Iowa to quantify germinable weed seedbank densities and compositions after 10+ years of cover cropping treatments. All five trials consisted of zero-tillage maize-soybean rotations managed with and without the inclusion of a yearly winter rye (Secale cerealL.) cover crop. Seedbank sampling was conducted in the early spring before crop planting at all locations, with three of the five trials having grown a soybean crop the preceding year, and two a maize crop. Two of the trials (both previously soybean) showed significant and biologically relevant decreases (4,070 and 927 seeds m−2, respectively) in seedbank densities in cover crop treatments compared to controls. In another two trials, one previously maize and one previously soybean, no difference was detected in seedbank densities. In the fifth trial (previously maize), there was a significant, but biologically unimportant increase of 349 seeds m−2. All five trials' weed communities were dominated by common waterhemp [Amaranthus tuberculatus(Moq.)], and changes in seedbank composition from cover-cropping were driven by changes in this species. Although previous studies have shown that increases in cover crop biomass are strongly correlated with weed suppression, in our study we did not find a relationship between seedbank changes and the mean amount of cover crop biomass produced over a 10-years period (experiment means ranging from 0.5 to 2.0 Mg ha−1yr−1), the stability of the cover crop biomass production, nor the amount produced going into the previous crop's growing season. We conclude that long-term use of a winter rye cover crop in a maize-soybean system has the potential to meaningfully reduce the size of weed seedbanks compared to winter fallows. However, identifying the mechanisms by which this occurs requires further research into processes such as seed predation and seed decay in cover cropped systems.
Comparison of the Isotopic Composition of Hg and Pb in Two Atmospheric Bioaccumulators in a Pyrenean Beech Forest (Iraty Forest, Western Pyrenees, France/Spain)
Authors: Barre Julien P. G. , Queipo-Abad Silvia , Sola-Larrañaga Cristina , Deletraz Gaëlle , Bérail Sylvain , Tessier Emmanuel , Elustondo Valencia David , Santamaría Jesús Miguel , de Diego Alberto , Amouroux David
Published: November 2020

Mercury (Hg) and lead (Pb) isotopic compositions were investigated in mosses and lichens collected in a large mountainous beech forest (Iraty Forest) located on the French-Spanish Pyrenean border. Hg isotopic signature in topsoil samples were also analyzed in selected sampling sites. This is the first work that uses the complementary information of both isotopic systems in two distinct atmospheric bioaccumulators. Mosses and lichens present characteristic accumulation due to their integration times, displaying different information on metal pollution over the area. Hg and Pb concentrations in annual moss shoots represent recent atmospheric accumulation, while whole lichen thalli integrates a process of accumulation over a longer period. Lead isotope ratios in mosses are consistent with reported data corresponding to the actual European atmospheric background (206Pb/207Pb ∼ 1.158), while Hg isotopic composition reflects potential uptake of both dry and wet Hg depositions. For lichens, Pb isotopic composition exhibits the contribution of a longer integration period of both industrial Pb emissions and legacy of leaded gasoline pollution. Hg isotopes in lichens discriminate two main groups: a larger one representing the background atmospheric contribution and a second one corresponding to unexpected higher Hg content. The similarities in odd and even Mass-independent fractionation of Hg isotopes between topsoils and lichens from the larger group, support the idea that foliage uptake is the main input of Hg in soils. The second group of lichens exhibits more negative δ202Hg (down to –4.69‰) suggesting a new source of fractionation in this area, probably related to lichens aging and/or stubble and grass fires due to pastoral activities. This study demonstrates that using both Hg and Pb isotopic signature in lichens and mosses allows to trace atmospheric sources and environmental pathways of these metals in forested ecosystems. This original data set in a remote environment provides also new information on the fate of atmospheric Pb and Hg depositions.
Layered MoS2 Grown on Anatase TiO2 {001} Promoting Interfacial Electron Transfer to Enhance Photocatalytic Evolution of H2 From H2S
Authors: Cai Qing , Wang Fang , Xiang Jianglai , Dan Meng , Yu Shan , Zhou Ying
Published: November 2020

The treatment of hazardous hydrogen sulfide (H2S) via photocatalysis technology has been known as one of the most promising green technologies. Photocatalytic production of hydrogen (H2) from H2S by two-dimensional (2D) semiconductor materials has gathered great attention owing to its large surface area and high catalytic activity. In this work, layered MoS2 has been successfully grown on TiO2 {001} surface to fabricate the 2D MoS2/TiO2 {001} composites for H2 evolution from H2S, which can be confirmed by the X-ray diffraction (XRD) and transmission electron microscopy (TEM) tests. Band structures and UV-Vis spectra provide important evidences that MoS2 loading can significantly narrow the band gap and broaden the light absorbance into the visible light region. Electron transfer is obviously visualized at the interface of MoS2/TiO2, resulting in the built-in potential from TiO2 to MoS2, which is determined by the density functional theory (DFT) calculations and X-ray photoelectron spectroscopy (XPS) test. Consequently, the photo-induced electrons and holes are accumulated at the sides of TiO2 and MoS2 under the illumination, respectively, which largely promote the interfacial electron transfer and prolong the lifetime of photo-generated electrons that participate in the photocatalytic reactions of H2 evolution from H2S. This efficient separation of photo-induced carriers can be further proved by photoluminescence (PL) spectra, photocurrent responses, and electrochemical impedance spectra. As a result, the photocatalytic activity of H2 evolution is largely increased by 9.4 times compared to the pristine TiO2. This study could offer a new and facile way to design highly efficient 2D photocatalysts for the application of H2S treatment.
Street Tree Density and Distribution: An International Analysis of Five Capital Cities
Authors: Smart Nicholas , Eisenman Theodore S. , Karvonen Andrew
Published: November 2020

Municipal leaders around the world are demonstrating significant interest in urban greening to realize a range of socioecological benefits. The urban greening toolkit often includes street trees, an essential component of urban design informed by historic legacies of both human and environmental factors. To date, there has been little comparative analysis of street tree density and distribution across international and intercontinental settings, and associated research has not been situated within the broader discussion of historical legacies. This study focuses on five capital cities (Ottawa, Stockholm, Buenos Aires, Paris, and Washington, DC) situated in two climate zones and it addresses two research questions: (1) What are the density and distribution of street trees across a given city and its street hierarchy? and (2) How do these metrics compare within and between cities by climate zone? The analysis draws upon up-to-date datasets from local authorities and includes geospatial analysis of street trees across hierarchical street classes within the central zones of each city. The results show clear differences in street tree density in cities within and between climate zones as well as differences in street tree distribution in cities within the same climate zone. Substantial differences within climate zones further suggest that cultural factors—including but not limited to urban form, aesthetic norms, and governance regimes—may play a pivotal role in the distribution and density of street trees. This illustrates the importance of place-specific cultural and environmental legacies as determinants of street tree density and distribution and supports further comparative research on the topic.
Adult-Onset Anti-Citrullinated Peptide Antibody-Negative Destructive Rheumatoid Arthritis Is Characterized by a Disease-Specific CD8+ T Lymphocyte Signature
Authors: Kelkka Tiina , Savola Paula , Bhattacharya Dipabarna , Huuhtanen Jani , Lönnberg Tapio , Kankainen Matti , Paalanen Kirsi , Tyster Mikko , Lepistö Maija , Ellonen Pekka , Smolander Johannes , Eldfors Samuli , Yadav Bhagwan , Khan Sofia , Koivuniemi Riitta , Sjöwall Christopher , Elo Laura L. , Lähdesmäki Harri , Maeda Yuka , Nishikawa Hiroyoshi , Leirisalo-Repo Marjatta , Sokka-Isler Tuulikki , Mustjoki Satu
Published: November 2020

Rheumatoid arthritis (RA) is a complex autoimmune disease targeting synovial joints. Traditionally, RA is divided into seropositive (SP) and seronegative (SN) disease forms, the latter consisting of an array of unrelated diseases with joint involvement. Recently, we described a severe form of SN-RA that associates with characteristic joint destruction. Here, we sought biological characteristics to differentiate this rare but aggressive anti-citrullinated peptide antibody-negative destructive RA (CND-RA) from early seropositive (SP-RA) and seronegative rheumatoid arthritis (SN-RA). We also aimed to study cytotoxic CD8+ lymphocytes in autoimmune arthritis. CND-RA, SP-RA and SN-RA were compared to healthy controls to reveal differences in T-cell receptor beta (TCRβ) repertoire, cytokine levels and autoantibody repertoires. Whole-exome sequencing (WES) followed by single-cell RNA-sequencing (sc-RNA-seq) was performed to study somatic mutations in a clonally expanded CD8+ lymphocyte population in an index patient. A unique TCRβ signature was detected in CND-RA patients. In addition, CND-RA patients expressed higher levels of the bone destruction-associated TNFSF14 cytokine. Blood IgG repertoire from CND-RA patients recognized fewer endogenous proteins than SP-RA patients’ repertoires. Using WES, we detected a stable mutation profile in the clonally expanded CD8+ T-cell population characterized by cytotoxic gene expression signature discovered by sc-RNA-sequencing. Our results identify CND-RA as an independent RA subset and reveal a CND-RA specific TCR signature in the CD8+ lymphocytes. Improved classification of seronegative RA patients underlines the heterogeneity of RA and also, facilitates development of improved therapeutic options for the treatment resistant patients.
HDAC6 Inhibition Alleviates CLL-Induced T-Cell Dysfunction and Enhances Immune Checkpoint Blockade Efficacy in the Eμ-TCL1 Model
Authors: Maharaj Kamira , Powers John J. , Mediavilla-Varela Melanie , Achille Alex , Gamal Wael , Quayle Steven , Jones Simon S. , Sahakian Eva , Pinilla-Ibarz Javier
Published: November 2020

Development of chronic lymphocytic leukemia (CLL) is associated with severe immune dysfunction. T-cell exhaustion, immune checkpoint upregulation, and increase of regulatory T cells contribute to an immunosuppressive tumor microenvironment. As a result, CLL patients are severely susceptible to infectious complications that increase morbidity and mortality. CLL B-cell survival is highly dependent upon interaction with the supportive tumor microenvironment. It has been postulated that the reversal of T-cell dysfunction in CLL may be beneficial to reduce tumor burden. Previous studies have also highlighted roles for histone deacetylase 6 (HDAC6) in regulation of immune cell phenotype and function. Here, we report for the first time that HDAC6 inhibition exerts beneficial immunomodulatory effects on CLL B cells and alleviates CLL-induced immunosuppression of CLL T cells. In the Eμ-TCL1 adoptive transfer murine model, genetic silencing or inhibition of HDAC6 reduced surface expression of programmed death-ligand 1 (PD-L1) on CLL B cells and lowered interleukin-10 (IL-10) levels. This occurred concurrently with a bolstered T-cell phenotype, demonstrated by alteration of coinhibitory molecules and activation status. Analysis of mice with similar tumor burden indicated that the majority of T-cell changes elicited by silencing or inhibition of HDAC6 in vivo are likely secondary to decrease of tumor burden and immunomodulation of CLL B cells. The data reported here suggest that CLL B cell phenotype may be altered by HDAC6-mediated hyperacetylation of the chaperone heat shock protein 90 (HSP90) and subsequent inhibition of the Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway. Based on the beneficial immunomodulatory activity of HDAC6 inhibition, we rationalized that HDAC6 inhibitors could enhance immune checkpoint blockade in CLL. Conclusively, combination treatment with ACY738 augmented the antitumor efficacy of anti-PD-1 and anti-PD-L1 monoclonal antibodies in the Eμ-TCL1 adoptive transfer murine model. These combinatorial antitumor effects coincided with an increased cytotoxic CD8+ T-cell phenotype. Taken together, these data highlight a role for HDAC inhibitors in combination with immunotherapy and provides the rationale to investigate HDAC6 inhibition together with immune checkpoint blockade for treatment of CLL patients.
The Combination of CD8αα and Peptide-MHC-I in a Face-to-Face Mode Promotes Chicken γδT Cells Response
Authors: Liu Yanjie , Chen Rong , Liang Ruiying , Sun Beibei , Wu Yanan , Zhang Lijie , Kaufman Jim , Xia Chun
Published: November 2020

The CD8αα homodimer is crucial to both thymic T cell selection and the antigen recognition of cytotoxic T cells. The CD8-pMHC-I interaction can enhance CTL immunityviastabilizing the TCR-pMHC-I interaction and optimizing the cross-reactivity and Ag sensitivity of CD8+T cells at various stages of development. To date, only human and mouse CD8-pMHC-I complexes have been determined. Here, we resolved the pBF2*1501 complex and the cCD8αα/pBF2*1501 and cCD8αα/pBF2*0401 complexes in nonmammals for the first time. Remarkably, cCD8αα/pBF2*1501 and the cCD8αα/pBF2*0401 complex both exhibited two binding modes, including an “antibody-like” mode similar to that of the known mammal CD8/pMHC-I complexes and a “face-to-face” mode that has been observed only in chickens to date. Compared to the “antibody-like” mode, the “face-to-face” binding mode changes the binding orientation of the cCD8αα homodimer to pMHC-I, which might facilitate abundant γδT cells to bind diverse peptides presented by limited BF2 alleles in chicken. Moreover, the forces involving in the interaction of cCD8αα/pBF2*1501 and the cCD8αα/pBF2*0401 are different in this two binding model, which might change the strength of the CD8-pMHC-I interaction, amplifying T cell cross-reactivity in chickens. The coreceptor CD8αα of TCR has evolved two peptide-MHC-I binding patterns in chickens, which might enhance the T cell response to major or emerging pathogens, including chicken-derived pathogens that are relevant to human health, such as high-pathogenicity influenza viruses.
Type I Interferon (IFN)-Regulated Activation of Canonical and Non-Canonical Signaling Pathways
Authors: Mazewski Candice , Perez Ricardo E. , Fish Eleanor N. , Platanias Leonidas C.
Published: November 2020

For several decades there has been accumulating evidence implicating type I interferons (IFNs) as key elements of the immune response. Therapeutic approaches incorporating different recombinant type I IFN proteins have been successfully employed to treat a diverse group of diseases with significant and positive outcomes. The biological activities of type I IFNs are consequences of signaling events occurring in the cytoplasm and nucleus of cells. Biochemical events involving JAK/STAT proteins that control transcriptional activation of IFN-stimulated genes (ISGs) were the first to be identified and are referred to as “canonical” signaling. Subsequent identification of JAK/STAT-independent signaling pathways, critical for ISG transcription and/or mRNA translation, are denoted as “non-canonical” or “non-classical” pathways. In this review, we summarize these signaling cascades and discuss recent developments in the field, specifically as they relate to the biological and clinical implications of engagement of both canonical and non-canonical pathways.
Semi-Active Scissors-Seat Suspension With Magneto-Rheological Damper
Authors: Zhu Hongtao , Rui Xiaoting , Yang Fufeng , Zhu Wei , Gu Junjie
Published: November 2020

A cab seat suspension with a magneto-rheological (MR) fluid damper is introduced in this paper. A unified-format model for the MR damper is proposed to describe the dynamic characteristics of the MR damper. Also, a simple force-inverse model and a viscous damping tracking model are used for the coil current solution. A digital integrator and an extended Kalman filter are respectively adopted to obtain the vibration velocity of the chair frame and the relative motion velocity of the MR damper piston. A new skyhook control base with viscous damping tracking is applied to the semi-active seat suspension. In the simulation, compared with passive seat suspension under different displacement excitation (2, 4, 6, 8 Hz-sine, and random), the acceleration root mean square of the seat suspension with the MR damper is reduced by 52.2%, 32.2%, 41.3%, 50.8%, and 34.6%, respectively. In the experiment, the acceleration root mean square is reduced by 11.2%, 41.2%, 45.8%, and 31.5%, respectively under different displacement excitation (2, 4, 6, and 8 Hz-sine).
Using Poloxamer® 407 as Building Block of Amphiphilic Poly(ether urethane)s: Effect of its Molecular Weight Distribution on Thermo-Sensitive Hydrogel Performances in the Perspective of Their Biomedical Application
Authors: Laurano Rossella , Abrami Michela , Grassi Mario , Ciardelli Gianluca , Boffito Monica , Chiono Valeria
Published: November 2020

Due to its hydroxyl terminal groups, Poloxamer® 407 (P407), a commercially available poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) triblock copolymer can be used as macrodiol for the synthesis of high molecular weight amphiphilic poly(ether urethane)s (PEUs). This work was aimed at studying the effect of P407 purification by removing PEO-PPO diblock copolymer by-products on the chemical properties of PEU polymer and the physical properties of PEU hydrogels. Removal of PEO-PPO diblock copolymers (P407_P) was found to preserve the thermo-responsiveness of resulting hydrogels, although slightly lower gelation onset temperature (Tonset) was found for P407_P (15.3°C) vs. P407 (16.7°C) hydrogels (25% w/V) as assessed through temperature ramp test. P407 and P407_P were then reacted with 1,6-diisocyanatohexane and 1,4-cyclohexanedimethanol to synthesize two different PEUs, coded as CHP407 and CHP407_P, respectively. Lower Number Average Molecular Weight (Mn¯) and higher polydispersity Index (D) was measured for CHP407 (Mn¯: 34 kDa, D: 1.6) respect to CHP407_P (Mn¯: 40 kDa, D: 1.4) as a consequence of macrodiol purification. CHP407_P hydrogels formed bigger micelles (43.9 ± 4.1 nm vs. 28.7 ± 4 nm) while showed similar critical micellar temperatures (22.1°C vs. 21.6°C) respect to CHP407 formulations. Sol-to-gel transition of CHP407 and CHP407_P hydrogels was similar while CHP407_P gelation time at 37°C was longer as assessed by tube inverting test. The rheological analysis showed slightly lower Tonset for CHP407_P hydrogels (15% w/V), probably due to larger micelle size, promoting micellar assembly. However, CHP407_P hydrogels showed a significantly lower critical strain than CHP407 hydrogels, as assessed by strain sweep test, suggesting their higher brittleness due to a lower density of intermicellar bridge chains. Nano-scale hydrogel characterization by Low-Field Nuclear Magnetic Resonance spectroscopy supported previous findings, showing lower spin-spin relaxation time (i.e., 1,259 ms) for CHP407_P than for CHP407 hydrogels (i.e., 1,560 ms) at 37°C, which suggested the formation of a more tightly packed network for CHP407_P than CHP407 hydrogel. Finally, lower swelling capability and resistance against dissolution were measured for CHP407_P hydrogels. Overall, the here‐reported results suggested that the heterogeneous structure in the CHP407 hydrogel network caused by the presence of diblock copolymer-based macrodiols improved PEU hydrogel properties in light of their applicability in the biomedical field.
The Impact of Periodontal Disease on Hospital Admission and Mortality During COVID-19 Pandemic
Authors: Larvin Harriet , Wilmott Sheryl , Wu Jianhua , Kang Jing
Published: November 2020

Introduction: COVID-19 has had a huge impact on society and healthcare and it has been suggested that people with periodontal disease are at risk of having worse outcomes from the disease. The aim of this study was to quantify the impact of periodontal disease on hospital admission and mortality during the COVID-19 pandemic.

Materials and Methods: The study extracted UK Biobank participants who had taken a COVID-19 test between March and June 2020 (n = 13,253), of which 1,616 were COVID-19 positive (12%) and 11,637 were COVID-19 negative (88%). Self-reported oral health indicators of painful or bleeding gums and loose teeth were used as surrogates for periodontal disease, participants who did not report any of the aforementioned indicators were used as controls. Multivariable logistic regressions were used to obtain crude and adjusted odds ratios of COVID-19 infection, subsequent hospital admission and mortality adjusted for demographics, BMI, biomarkers, lifestyle and co-morbidities.

Results: Painful gums, bleeding gums and loose teeth were reported in 2.7, 11.2 and 3.3% of participants with COVID-19 infection, respectively. Risk of COVID-19 infection in participants with painful or bleeding gums and loose teeth compared to controls was not increased (odds ratio [OR]: 1.10, 95% CI: 0.72–1.69; OR: 1.15, 95% CI: 0.84–1.59). COVID-19 positive participants with painful or bleeding gums had a higher risk of mortality (OR: 1.71, 95% CI: 1.05–2.72) but not hospital admission (OR: 0.90, 95% CI: 0.59–1.37). Participants with loose teeth did not show higher risk of hospital admission or mortality compared to the control group (OR = 1.55, 95% CI: 0.87–2.77; OR: 1.85; 95% CI: 0.92–2.72).

Conclusion: There was insufficient evidence to link periodontal disease with an increased risk of COVID-19 infection. However, amongst the COVID-19 positive, there was significantly higher mortality for participants with periodontal disease. Utilization of linked dental and hospital patient records would improve the understanding of the impact of periodontal disease on COVID-19 related outcomes.
A Novel Major Pilin Subunit Protein FimM Is Involved in Adhesion of Bifidobacterium longum BBMN68 to Intestinal Epithelial Cells
Authors: Xiong Yao , Zhai Zhengyuan , Lei Yuanqiu , Xiao Bingbing , Hao Yanling
Published: November 2020

Adhesion to the gastrointestinal tract is considered to be important for bifidobacteria to colonize the human gut and exert their probiotic effects. Some cell surface proteins of bifidobacteria, known as adhesins, play critical roles in the binding to host cells or the extracellular matrix (ECM). To elucidate the mechanisms associated with the adhesion of Bifidobacterium longum BBMN68, a centenarian originated potential probiotic, PSORTdb was employed to identify putative extracellular localized proteins in the B. longum BBMN68. Of the 560 predicted extracellular proteins, 21 were further identified as putative adhesion proteins using the conserved domain database of NCBI, and four were successfully overexpressed in the heterologous host, Lactococcus lactis NZ9000. Notably, a recombinant strain expressing FimM showed a significantly increased adhesive affinity for both HT-29 and mucus-secreting LS174T goblet cells (2.2- and 5.4-fold higher than that of the control strain, respectively). Amino acid sequence alignment showed that FimM is a major pilin subunit protein containing a Cna-B type domain and a C-terminal LPKTG sequence. However, in silico analysis of the fimM-coding cluster revealed that BBMN68_RS10200, encoding a pilus-specific class C sortase, was a pseudogene, indicating that FimM may function as a surface adhesin that cannot polymerize into a pili-like structure. Immunogold electron microscopy results further confirmed that FimM localized to the surface of L. lactis NZfimM and B. longum BBMN68 but did not assemble into pilus filaments. Moreover, the adhesive affinity of L. lactis NZfimM to fibronectin, fibrinogen, and mucin were 3.8-, 2.1-, and 3.1-fold higher than that of the control. The affinity of FimM for its attachment receptors was further verified through an inhibition assay using anti-FimM antibodies. In addition, homologs of FimM were found in Bifidobacterium bifidum 85B, Bifidobacterium gallinarum CACC 514, and 23 other B. longum strains by sequence similarity analysis using BLASTP. Our results suggested that FimM is a novel surface adhesin that is mainly present in B. longum strains.
Diversity and Geographic Distribution of Ligninolytic Fungi Associated With Castanopsis sieboldii Leaf Litter in Japan
Authors: Osono Takashi , Matsuoka Shunsuke , Hirose Dai
Published: November 2020

The diversity and geographic pattern of ligninolytic fungi were investigated within the distribution range of an evergreen tree,Castanopsis sieboldii(Fagaceae), in Japan. Fungal isolates obtained from 18 sites in subtropical and temperate regions in Japan were classified into 50 operational taxonomic units in Ascomycota and Basidiomycota according to the base sequence of the rDNA internal transcribed spacer region. Ordination by nonmetric multidimensional scaling showed the separation of fungal compositions between the study sites which was significantly related to the latitude, longitude, and mean annual temperature (MAT) of the study sites. We applied variation partitioning to separate the magnitude of the climatic, spatial, and leaf property factors and found the roles of MAT and spatial factors in structuring fungal assemblages, suggesting the importance of both niche processes and such non-niche processes as priority effect and dispersal limitation. The bleached area on leaf litter was greater at sites with higher MAT and precipitation located at lower latitudes and at sites where some major ligninolytic fungi occurred at greater relative frequencies, indicating that not only the climatic conditions but also the biogeographic patterns of distribution of ligninolytic fungi influence the decomposition of lignin in leaf litter.
A New Cold-Active Glucose Oxidase From Penicillium: High-Level Expression and Application in Fish Preservation
Authors: Yuan Mingxue , Ning Chen , Yang Suxiao , Liang Qingping , Mou Haijin , Liu Zhemin
Published: November 2020

Glucose oxidase (GOx) with high enzyme activity at low temperature (4°C) is potentially useful for food preservation, especially for aquatic products preservation. A cold-active GOx with approximately 83% similarity to known protein sequences, was isolated fromPenicilliumsp. MX3343 and expressed inPichia pastorisX33. Through high cell density fermentation, the yield of recombinant enzyme (named GOxP5) reached 458.6 U/mL. GOxP5showed optimal activity at 30°C and pH 5.5, and was stable at a broad pH range from pH 2–6. Moreover, GOxP5could maintain 72% maximum activity at 4°C, suggesting its application for the preservation of aquatic products at low-temperatures. Importantly, GOxP5showed a good antimicrobial effect against common fish pathogenic bacteria (Listeria monocytogenesandVibrio parahaemolyticus). Moreover, sensory, microbiological (total bacterial count), and physicochemical (total volatile basic nitrogen and pH) systematic analyses proved GOxP5to be an excellent freshness preserving agent in the context of the grass carp. These favorable enzymatic properties of GOxP5make it potentially useful in food biopreservation, and the effect was better compared to the commonly used chemical preservatives.
Effect of Aggressive Experience in Female Syrian Hamsters on Glutamate Receptor Expression in the Nucleus Accumbens
Authors: Borland Johnathan M. , Kim Ellen , Swanson Samuel P. , Rothwell Patrick E. , Mermelstein Paul G. , Meisel Robert L.
Published: November 2020

Our social relationships determine our health and well-being. In rodent models, there is now strong support for the rewarding properties of aggressive or assertive behaviors to be critical for the expression and development of adaptive social relationships, buffering from stress and protecting from the development of psychiatric disorders such as depression. However, due to the false belief that aggression is not a part of the normal repertoire of social behaviors displayed by females, almost nothing is known about the neural mechanisms mediating the rewarding properties of aggression in half the population. In the following study, using Syrian hamsters as a well-validated and translational model of female aggression, we investigated the effects of aggressive experience on the expression of markers of postsynaptic structure (PSD-95, Caskin I) and excitatory synaptic transmission (GluA1, GluA2, GluA4, NR2A, NR2B, mGluR1a, and mGluR5) in the nucleus accumbens (NAc), caudate putamen and prefrontal cortex. Aggressive experience resulted in an increase in PSD-95, GluA1 and the dimer form of mGluR5 specifically in the NAc 24 h following aggressive experience. There was also an increase in the dimer form of mGluR1a 1 week following aggressive experience. Aggressive experience also resulted in an increase in the strength of the association between these postsynaptic proteins and glutamate receptors, supporting a common mechanism of action. In addition, 1 week following aggressive experience there was a positive correlation between the monomer of mGluR5 and multiple AMPAR and NMDAR subunits. In conclusion, we provide evidence that aggressive experience in females results in an increase in the expression of postsynaptic density, AMPARs and group I metabotropic glutamate receptors, and an increase in the strength of the association between postsynaptic proteins and glutamate receptors. This suggests that aggressive experience may result in an increase in excitatory synaptic transmission in the NAc, potentially encoding the rewarding and behavioral effects of aggressive interactions.
Sex Differences in the Patterns and Predictors of Cognitive Function in HIV
Authors: Rubin Leah H. , Sundermann Erin E. , Dastgheyb Raha , Buchholz Alison S. , Pasipanodya Elizabeth , Heaton Robert K. , Grant Igor , Ellis Ronald , Moore David J.
Published: November 2020

Despite advancements in antiretroviral therapy, mild cognitive deficits persist in nearly half of people with HIV (PWH). The profile of impairment in HIV is highly variable with deficits observed in a range of cognitive domains. Despite evidence of greater cognitive impairment among women with HIV (WWH) vs. men with HIV (MWH), it is unclear how MWH and WWH differ in the type of cognitive impairment and in risk factors associated with cognitive impairment profiles. In a large and well-characterized sample of PWH, we used machine learning to identify profiles of cognitive functioning and their associated factors overall and within sex. Participants included 1,666 PWH (201 WWH; 1,465 MMH) from the HIV Neurobehavioral Research Program who completed a neuropsychological test battery at their baseline visits. Using demographically-adjusted T-scores from 13 test outcomes assessing motor skills, executive functioning, attention/working memory, episodic learning and memory, verbal fluency, and processing speed, we used Kohonen self-organizing maps to identify patterns of high-dimensional data by mapping participants to similar nodes based on T-scores (MCLUST R package). Random forest models were used to determine how sociodemographic (e.g., age, education), clinical (e.g., depressive symptoms, substance use disorder), and biological (e.g., HIV disease characteristics) factors differentially related to membership within a cognitive profile. All analyses were repeated within sex. Three cognitive profiles were identified overall and within each sex. Overall and within MWH, there were unimpaired and global weakness profiles. The third profile in the total sample demonstrated relatively weak auditory attention whereas in MWH showed relative strengths in attention and processing speed. Conversely, there was no unimpaired profile among WWH. Rather, WWH demonstrated separate profiles reflecting weakness in motor skills, a relative weakness in learning and delayed recall, and global weaknesses with spared recognition memory. Despite different cognitive profiles by sex, the most discriminative factors were similar between men and women and included reading level (cognitive reserve), current and nadir CD4 count, plasma HIV viral load, duration of HIV disease, age, depressive symptoms, and race/ethnicity. Findings fill a knowledge gap concerning sex differences in cognitive impairment in PWH and inform personalized risk reduction and therapeutic strategies.
Resting Heart Rate Variability Is Associated With Subsequent Orthostatic Hypotension: Comparison Between Healthy Older People and Patients With Rapid Eye Movement Sleep Behavior Disorder
Authors: Sumi Yukiyoshi , Nakayama Chikao , Kadotani Hiroshi , Matsuo Masahiro , Ozeki Yuji , Kinoshita Takafumi , Goto Yuki , Kano Manabu , Yamakawa Toshitaka , Hasegawa-Ohira Masako , Ogawa Keiko , Fujiwara Koichi
Published: November 2020

Background: Orthostatic hypotension (OH) caused by autonomic dysfunction is a common symptom in older people and patients with idiopathic rapid eye movement sleep behavior disorder (iRBD). The orthostatic challenge test is a standard autonomic function test that measures a decrease of blood pressure during a postural change from supine to standing positions. Although previous studies have reported that changes in heart rate variability (HRV) are associated with autonomic dysfunction, no study has investigated the relationship between HRV before standing and the occurrence of OH in an orthostatic challenge test. This study aims to examine the connection between HRV in the supine position and the occurrence of OH in an orthostatic challenge test.

Methods: We measured the electrocardiograms of patients with iRBD and healthy older people during an orthostatic challenge test, in which the supine and standing positions were held for 15 min, respectively. The subjects were divided into three groups: healthy controls (HC), OH-negative iRBD [OH (–) iRBD], and OH-positive iRBD [OH (+) iRBD]. HRV measured in the supine position during the test were calculated by time-domain analysis and Poincaré plots to evaluate the autonomic dysfunction.

Results: Forty-two HC, 12 OH (–) iRBD, and nine OH (+) iRBD subjects were included. HRV indices in the OH (–) and the OH (+) iRBD groups were significantly smaller than those in the HC group. The multivariate logistic regression analysis for OH identification for the iRBD groups showed the model whose inputs were the HRV indices, i.e., standard deviation 2 (SD2) and the percentage of adjacent intervals that varied by more than 50 ms (pNN50), had a receiver operating characteristic curve with area under the curve of 0.840, the sensitivity to OH (+) of 1.000, and the specificity to OH (–) of 0.583 (p = 0.023).

Conclusions: This study showed the possibility that short-term HRV indices in the supine position would predict subsequent OH in iRBD patients. Our results are of clinical importance in terms of showing the possibility that OH can be predicted using only HRV in the supine position without an orthostatic challenge test, which would improve the efficiency and safety of testing.
A Novel Transfer Support Matrix Machine for Motor Imagery-Based Brain Computer Interface
Authors: Chen Yan , Hang Wenlong , Liang Shuang , Liu Xuejun , Li Guanglin , Wang Qiong , Qin Jing , Choi Kup-Sze
Published: November 2020

In recent years, emerging matrix learning methods have shown promising performance in motor imagery (MI)-based brain-computer interfaces (BCIs). Nonetheless, the electroencephalography (EEG) pattern variations among different subjects necessitates collecting a large amount of labeled individual data for model training, which prolongs the calibration session. From the perspective of transfer learning, the model knowledge inherent in reference subjects incorporating few target EEG data have the potential to solve the above issue. Thus, a novel knowledge-leverage-based support matrix machine (KL-SMM) was developed to improve the classification performance when only a few labeled EEG data in the target domain (target subject) were available. The proposed KL-SMM possesses the powerful capability of a matrix learning machine, which allows it to directly learn the structural information from matrix-form EEG data. In addition, the KL-SMM can not only fully leverage few labeled EEG data from the target domain during the learning procedure but can also leverage the existing model knowledge from the source domain (source subject). Therefore, the KL-SMM can enhance the generalization performance of the target classifier while guaranteeing privacy protection to a certain extent. Finally, the objective function of the KL-SMM can be easily optimized using the alternating direction method of multipliers method. Extensive experiments were conducted to evaluate the effectiveness of the KL-SMM on publicly available MI-based EEG datasets. Experimental results demonstrated that the KL-SMM outperformed the comparable methods when the EEG data were insufficient.
Clinicopathologic Features and Prognosis of BRAF Mutated Colorectal Cancer Patients
Authors: Guan Wen-Long , Qiu Miao-Zhen , He Cai-Yun , Yang Li-Qiong , Jin Ying , Wang Zhi-Qiang , Li Yu-Hong , Xu Rui-Hua , Wang Feng-Hua
Published: November 2020

Background:BRAFV600E mutation is associated with poor prognosis of colorectal cancer (CRC) patients, but the comparison of clinic-pathologic features between V600E and non-V600E mutation was not well-known in CRC patients. The aim of this study is to evaluate the clinical and pathological features, prognostic value of BRAF mutations in CRC.

Methods: We conducted a retrospective study to characterize the clinical and pathological features and survival of patients with BRAF mutated CRC. Patients were classified according to BRAF status as BRAFV600E mutation and non-V600E mutations. Difference of characteristics and survival between the two groups was analyzed.

Results: There was no significant difference in gender, family history, location of primary tumor, metastatic sites between patients with BRAF-V600E mutation and non-V600E mutations. Patients with V600E mutation were younger than those with non-V600E mutations (p = 0.002). Patients with BRAFV600E mutation showed a poorer outcome than those with non-V600E mutations (23.1 vs. 49.9 months, respectively, p = 0.0024). Lack of CDX2 expression was associated with worse prognosis (mOS: 9.4 m vs. not reached, respectively, p = 0.016). Status of V600E mutation did not affect the mPFS and ORR of first-line or second-line treatment.

Conclusion:BRAFV600E mutation defines a distinct subgroup of CRC with worse prognosis. Lack of CDX2 expression is associated with poor OS. Status of V600E mutation did not affect the mPFS of first-line or second-line treatment.
Isotropic Expansion of the Intraprostatic Gross Tumor Volume of Primary Prostate Cancer Patients Defined in MRI—A Correlation Study With Whole Mount Histopathological Information as Reference
Authors: Kramer Maria , Spohn Simon K. B. , Kiefer Selina , Ceci Lara , Sigle August , Oerther Benedict , Schultze-Seemann Wolfgang , Gratzke Christian , Bock Michael , Bamberg Fabian , Grosu Anca L. , Benndorf Matthias , Zamboglou Constantinos
Published: November 2020

Introduction

An accurate delineation of the intraprostatic gross tumor volume (GTV) is of importance for focal treatment in patients with primary prostate cancer (PCa). Multiparametric MRI (mpMRI) is the standard of care for lesion detection but has been shown to underestimate GTV. This study investigated how far the GTV has to be expanded in MRI in order to reach concordance with the histopathological reference and whether this strategy is practicable in clinical routine.

Patients and Methods

Twenty-two patients with planned prostatectomy and preceded 3 Tesla mpMRI were prospectively examined. After surgery, PCa contours delineated on histopathological slides (GTV-Histo) were superimposed on MRI using ex-vivo imaging as support for co-registration. According to the PI-RADSv2 classification, GTV was manually delineated in MRI (GTV-MRI) by two experts in consensus. For volumetric analysis, we compared GTV-MRI and GTV-Histo. Subsequently, we isotropically enlarged GTV-MRI in 1 mm increments within the prostate and also compared those with GTV-Histo regarding the absolute volumes. For evaluating the spatial accuracy, we considered the coverage ratio of GTV-Histo, the Sørensen–Dice coefficient (DSC), as well as the contact with the urethra.

Results

In 19 of 22 patients MRI underestimated the intraprostatic tumor volume compared to histopathological reference: median GTV-Histo (4.7 cm3, IQR: 2.5–18.8) was significantly (p<0.001) lager than median GTV-MRI (2.6 cm3, IQR: 1.2–6.9). A median expansion of 1 mm (range: 0–4 mm) adjusted the initial GTV-MRI to at least the volume of GTV-Histo (GTVexp-MRI). Original GTV-MRI and expansion with 1, 2, 3, and 4 mm covered in median 39% (IQR: 2%–78%), 62% (10%–91%), 70% (15%–95%), 80% (21–100), 87% (25%–100%) of GTV-Histo, respectively. Best DSC (median: 0.54) between GTV-Histo and GTV-MRI was achieved by median expansion of 2 mm. The urethra was covered by initial GTVs-MRI in eight patients (36%). After applying an expansion with 2 mm the urethra was covered in one more patient by GTV-MRI.

Conclusion

Using histopathology as reference, we demonstrated that MRI underestimates intraprostatic tumor volume. A 2 mm–expansion may improve accurate GTV-delineation while respecting the balance between histological tumor coverage and overtreatment.