bluuebunny/crossref_metadata_2025 huggingface dataset:
Online Failure Diagnosis in Interdependent Networks
Authors: Shiri Davood , Akbari Vahid
Published: February 2021

In interdependent networks, nodes are connected to each other with respect to their failure dependency relations. As a result of this dependency, a failure in one of the nodes of one of the networks within a system of several interdependent networks can cause the failure of the entire system. Diagnosing the initial source of the failure in a collapsed system of interdependent networks is an important problem to be addressed. We study an online failure diagnosis problem defined on a collapsed system of interdependent networks where the source of the failure is at an unknown node (v). In this problem, each node of the system has a positive inspection cost and the source of the failure is diagnosed when v is inspected. The objective is to provide an online algorithm which considers dependency relations between nodes and diagnoses v with minimum total inspection cost. We address this problem from worst-case competitive analysis perspective for the first time. In this approach, solutions which are provided under incomplete information are compared with the best solution that is provided in presence of complete information using the competitive ratio (CR) notion. We give a lower bound of the CR for deterministic online algorithms and prove its tightness by providing an optimal deterministic online algorithm. Furthermore, we provide a lower bound on the expected CR of randomized online algorithms and prove its tightness by presenting an optimal randomized online algorithm. We prove that randomized algorithms are able to obtain better CR compared to deterministic algorithms in the expected sense for this online problem.
Phantom Pains and Prosthetic Narratives
Authors: Minnis Alastair
Published: February 2021

'Phantom limb pain' designates the sensations which seem to emanate from limbs that in reality are missing. The phrase was coined by the American Civil War surgeon, Weir Mitchell, in reference to his fictional amputee, George Dedlow. Contemporary neuroscience holds that the brain encloses a schema which covers the whole body, and asserts its unity even if certain parts are missing. Reading backwards from Dedlow's sufferings, Alastair Minnis traces the medieval precedents and parallels, focusing on Augustine and Dante, who subscribed to the notion of a 'body in the soul'. Dante's souls in purgatory self-prosthesize with aerial phantoms as they long for the full embodiment which only the resurrection can bring. Is a complete body necessary for personhood? And how can the gamut of human feelings be run if parts or the entirety of one's body does not exist? Combining medieval studies and contemporary neuroscience, this absorbing study explores the fascinating and surprising history of phantom pain.
Avoiding the Subject
Authors: Clemens Justin , Pettman Dominic
Published: February 2021

What can Roger Rabbit tell us about the Second Gulf War? What can a woman married to the Berlin Wall tell us about posthumanism and inter-subjectivity? What can DJ Shadow tell us about the end of history? What can our local bus route tell us about the fortification of the West? What can Reality TV tell us about the crisis of contemporary community? And what can unauthorized pictures of Osama Bin Laden tell us about new methods of popular propaganda? These are only some of the thought-provoking questions raised in -Avoiding the Subject,- which highlights the feedback-loops between philosophy, technology, and politics in today's mediascape.
On Ruelle’s property
Authors: HUO SHENGJIN , ZINSMEISTER MICHEL
Published: February 2021

In this paper we investigate the range of validity of Ruelle’s property. First, we show that every finitely generated Fuchsian group has Ruelle’s property. We also prove the existence of an infinitely generated Fuchsian group satisfying Ruelle’s property. Concerning the negative results, we first generalize Astala and Zinsmeister’s results [Mostow rigidity and Fuchsian groups. C. R. Math. Acad. Sci. Paris311 (1990), 301–306; Teichmüller spaces and BMOA. Math. Ann.289 (1991), 613–625] by proving that all convergence-type Fuchsian groups of the first kind fail to have Ruelle’s property. Finally, we give some results about second-kind Fuchsian groups. [-3.2pc]
Intimately Old: From an Embodied to Emplaced Feminist Approach to Aging
Authors: Finlay Jessica
Published: February 2021

Aging transcends and intersects all structured social differences as a fluid complex of positionalities: a temporal situatedness in relation to gender, class, race, and sexuality. Age's operation as an organizing principle of power remains undertheorized in feminist philosophy. This article employs a geographical lens to spatialize feminist thought on old age to enrich understanding of factors underpinning expectations and practices of what particular bodies can and should do in particular spaces. Vignettes from twelve months of ethnographic fieldwork with six older individuals in a midwestern American city demonstrate the utility of advancing not just an embodied feminist philosophy of aging, but one that is emplaced to deepen understanding of the body as situated in time and space. A person's situatedness in dynamic place-events, ranging from daily life at home and engagement in supportive social spaces to experiences of discrimination and even inclement weather, produce distinct ways of being old. Investigating intimate geographies of later life from the micro to macro scale can help destabilize and challenge the objectification, control, and Othering of old people, the majority of whom are female. This article contributes greater “body a-where-ness” to feminist philosophy and stimulates novel investigation into the spatiotemporal situatedness of later life.
Mortality models incorporating long memory for life table estimation: a comprehensive analysis
Authors: Yan Hongxuan , Peters Gareth W. , Chan Jennifer
Published: February 2021

Mortality projection and forecasting of life expectancy are two important aspects of the study of demography and life insurance modelling. We demonstrate in this work the existence of long memory in mortality data. Furthermore, models incorporating long memory structure provide a new approach to enhance mortality forecasts in terms of accuracy and reliability, which can improve the understanding of mortality. Novel mortality models are developed by extending the Lee–Carter (LC) model for death counts to incorporate a long memory time series structure. To link our extensions to existing actuarial work, we detail the relationship between the classical models of death counts developed under a Generalised Linear Model (GLM) formulation and the extensions we propose that are developed under an extension to the GLM framework known in time series literature as the Generalised Linear Autoregressive Moving Average (GLARMA) regression models. Bayesian inference is applied to estimate the model parameters. The Deviance Information Criterion (DIC) is evaluated to select between different LC model extensions of our proposed models in terms of both in-sample fits and out-of-sample forecasts performance. Furthermore, we compare our new models against existing models structures proposed in the literature when applied to the analysis of death count data sets from 16 countries divided according to genders and age groups. Estimates of mortality rates are applied to calculate life expectancies when constructing life tables. By comparing different life expectancy estimates, results show the LC model without the long memory component may provide underestimates of life expectancy, while the long memory model structure extensions reduce this effect. In summary, it is valuable to investigate how the long memory feature in mortality influences life expectancies in the construction of life tables.
Dual targeting of MTOR as a novel therapeutic approach for high-risk B-cell acute lymphoblastic leukemia
Authors: Ge Zheng , Song Chunhua , Ding Yali , Tan Bi-Hua , Desai Dhimant , Sharma Arati , Gowda Raghavendra , Yue Feng , Huang Suming , Spiegelman Vladimir , Payne Jonathon L. , Reeves Mark E. , Iyer Soumya , Dhanyamraju Pavan Kumar , Imamura Yuka , Bogush Daniel , Bamme Yevgeniya , Yang Yiping , Soliman Mario , Kane Shriya , Dovat Elanora , Schramm Joseph , Hu Tommy , McGrath Mary , Chroneos Zissis C. , Payne Kimberly J. , Gowda Chandrika , Dovat Sinisa
Published: February 2021

Children of Hispanic/Latino ancestry have increased incidence of high-risk B-cell acute lymphoblastic leukemia (HR B-ALL) with poor prognosis. This leukemia is characterized by a single-copy deletion of the IKZF1 (IKAROS) tumor suppressor and increased activation of the PI3K/AKT/mTOR pathway. This identifies mTOR as an attractive therapeutic target in HR B-ALL. Here, we report that IKAROS represses MTOR transcription and IKAROS’ ability to repress MTOR in leukemia is impaired by oncogenic CK2 kinase. Treatment with the CK2 inhibitor, CX-4945, enhances IKAROS activity as a repressor of MTOR, resulting in reduced expression of MTOR in HR B-ALL. Thus, we designed a novel therapeutic approach that implements dual targeting of mTOR: direct inhibition of the mTOR protein (with rapamycin), in combination with IKAROS-mediated transcriptional repression of the MTOR gene (using the CK2 inhibitor, CX-4945). Combination treatment with rapamycin and CX-4945 shows synergistic therapeutic effects in vitro and in patient-derived xenografts from Hispanic/Latino children with HR B-ALL. These data suggest that such therapy has the potential to reduce the health disparity in HR B-ALL among Hispanic/Latino children. The dual targeting of oncogene transcription, combined with inhibition of the corresponding oncoprotein provides a paradigm for a novel precision medicine approach for treating hematological malignancies.
A dismantling study on imaginal retraining in smokers
Authors: Moritz Steffen , Gehlenborg Josefine , Wirtz Janina , Ascone Leonie , Kühn Simone
Published: February 2021

Imaginal retraining is a noncomputerized variant of cognitive bias modification, an intervention aimed at reducing craving in substance use disorders and behavioral addictions. We conducted a dismantling study to elucidate which of its multiple components are effective and hence essential ingredients of the training and which are ineffective (and hence perhaps dispensable) in reducing craving. We randomized 187 smokers to one out of six conditions that instructed participants to perform a brief intervention. In four of these, participants were instructed to perform isolated components of the imaginal retraining protocol, and in the two other conditions participants either suppressed or simply observed (control condition) the image of a cigarette. Before and after the intervention, participants were asked to rate their level of craving and how pleasant they found three smoking-related images. We examined within-group changes by means of paired t-tests separately across conditions (trial registration: DRKS00021044). Mental distancing from cigarettes (without a corresponding actual physical movement; non-motor retraining) led to a significant decline in craving (paired t-test), which remained significant when compared to the control condition. The effects of other components of the retraining were less consistent. The present study shows that a single therapeutic “dose” of distinct components involved in imaginal retraining can reduce craving for cigarettes. Future trials should investigate the effectiveness of components of imaginal retraining not yet tested (e.g., mood induction) and whether combinations and repetition of single components strengthen or dilute efficacy.
Mechanotransduction via the coordinated actions of integrins, PI3K signaling and Connexin hemichannels
Authors: Riquelme Manuel A. , Gu Sumin , Hua Rui , Jiang Jean X.
Published: February 2021

Mechanical loading opens connexin 43 (Cx43) hemichannels (HCs), leading to the release of bone anabolic molecules, such as prostaglandins, from mechanosensitive osteocytes, which is essential for bone formation and remodeling. However, the mechanotransduction mechanism that activates HCs remains elusive. Here, we report a unique pathway by which mechanical signals are effectively transferred between integrin molecules located in different regions of the cell, resulting in HC activation. Both integrin α5 and αV were activated upon mechanical stimulation via either fluid dropping or flow shear stress (FSS). Inhibition of integrin αV activation or ablation of integrin α5 prevented HC opening on the cell body when dendrites were mechanically stimulated, suggesting mechanical transmission from the dendritic integrin αV to α5 in the cell body during HC activation. In addition, HC function was compromised in vivo, as determined by utilizing an antibody blocking αV activation and α5-deficient osteocyte-specific knockout mice. Furthermore, inhibition of integrin αV activation, but not that of α5, attenuated activation of the phosphoinositide 3-kinase (PI3K)-protein kinase B (AKT) signaling pathway upon mechanical loading, and the inhibition of PI3K/AKT activation blocked integrin α5 activation and HC opening. Moreover, HC opening was blocked only by an anti-integrin αV antibody at low but not high FSS levels, suggesting that dendritic αV is a more sensitive mechanosensor than α5 for activating HCs. Together, these results reveal a new molecular mechanism of mechanotransduction involving the coordinated actions of integrins and PI3K/AKT in osteocytic dendritic processes and cell bodies that leads to HC opening and the release of key bone anabolic factors.
TGFβ signaling curbs cell fusion and muscle regeneration
Authors: Girardi Francesco , Taleb Anissa , Ebrahimi Majid , Datye Asiman , Gamage Dilani G. , Peccate Cécile , Giordani Lorenzo , Millay Douglas P. , Gilbert Penney M. , Cadot Bruno , Le Grand Fabien
Published: February 2021

Muscle cell fusion is a multistep process involving cell migration, adhesion, membrane remodeling and actin-nucleation pathways to generate multinucleated myotubes. However, molecular brakes restraining cell–cell fusion events have remained elusive. Here we show that transforming growth factor beta (TGFβ) pathway is active in adult muscle cells throughout fusion. We find TGFβ signaling reduces cell fusion, regardless of the cells’ ability to move and establish cell-cell contacts. In contrast, inhibition of TGFβ signaling enhances cell fusion and promotes branching between myotubes in mouse and human. Exogenous addition of TGFβ protein in vivo during muscle regeneration results in a loss of muscle function while inhibition of TGFβR2 induces the formation of giant myofibers. Transcriptome analyses and functional assays reveal that TGFβ controls the expression of actin-related genes to reduce cell spreading. TGFβ signaling is therefore requisite to limit mammalian myoblast fusion, determining myonuclei numbers and myofiber size.
An origin identification model for labeling of shiitake (Lentinula edodes)
Authors: Chung Ill-Min , Kim Yun-Ju , Kwon Chang , Moon Hee-Sung , Han Jae-Gu , Kong Won-Sik , Kim Seung-Hyun
Published: February 2021

With the increasing globalization of the food trade across countries and continents, reliable identification of the geographical origin of products is critical. In this study, we describe the limitations of the current origin labeling system for non-soil-based agricultural products and suggest alternative strategies for the identification of the geographical origin of such products. An origin identification model based on stable isotope ratio analysis combined with discriminant analysis is used to evaluate the similarities and dissimilarities between domestic and foreign shiitake mushrooms, including Chinese inoculated sawdust blocks and Chinese origin. The results show a classification sensitivity of 92.0%, classification specificity of 91.5%, and overall accuracy of 93.5%. In particular, δ15N was the most important isotope marker for the identification of the origin of shiitake mushrooms. Hence, the current origin labeling system for mushroom species has to be revised to establish fair trade and avoid improper origin labeling in the global shiitake market.
Dynamical system analysis of FLRW models with Modified Chaplygin gas
Authors: Yılmaz Ali Osman , Güdekli Ertan
Published: February 2021

We investigate Friedmann–Lamaitre–Robertson–Walker (FLRW) models with modified Chaplygin gas and cosmological constant, using dynamical system methods. We assume $$p=(\gamma -1)\mu -\dfrac{A}{\mu ^\alpha }$$

p
=

(
γ
-
1
)

μ
-

A

μ
α

as equation of state where $$\mu$$
μ
is the matter-energy density, p is the pressure, $$\alpha$$
α
is a parameter which can take on values $$0<\alpha \le 1$$

0
<
α
≤
1

as well as A and $$\gamma$$
γ
are positive constants. We draw the state spaces and analyze the nature of the singularity at the beginning, as well as the fate of the universe in the far future. In particular, we address the question whether there is a solution which is stable for all the cases.
Cross-sex hormone treatment and own-body perception: behavioral and brain connectivity profiles
Authors: Khorashad Behzad S. , Manzouri Amirhossein , Feusner Jamie D. , Savic Ivanka
Published: February 2021

Referrals for gender dysphoria (GD), characterized by a distressful incongruence between gender identity and at-birth assigned sex, are steadily increasing. The underlying neurobiology, and the mechanisms of the often-beneficial cross-sex hormone treatment are unknown. Here, we test hypothesis that own body perception networks (incorporated in the default mode network—DMN, and partly in the salience network—SN), are different in trans-compared with cis-gender persons. We also investigate whether these networks change with cross-sex hormone treatment. Forty transmen (TrM) and 25 transwomen (TrW) were scanned before and after cross-sex hormone institution. We used our own developed Body Morph test (BM), to assess the perception of own body as self. Fifteen cisgender persons were controls. Within and between-group differences in functional connectivity were calculated using independent components analysis within the DMN, SN, and motor network (a control network). Pretreatment, TrM and TrW scored lower “self” on the BM test than controls. Their functional connections were weaker in the anterior cingulate-, mesial prefrontal-cortex (mPFC), precuneus, the left angular gyrus, and superior parietal cortex of the DMN, and ACC in the SN “Self” identification and connectivity in the mPFC in both TrM and TrW increased from scan 1 to 2, and at scan 2 no group differences remained. The neurobiological underpinnings of GD seem subserved by cerebral structures composing major parts of the DMN.
Paternal effects in the initiation of migratory behaviour in birds
Authors: Méndez V. , Gill J. A. , Þórisson B. , Vignisson S. R. , Gunnarsson T. G. , Alves J. A.
Published: February 2021

What determines why some birds migrate and others do not? This question is fundamental to understanding how migratory systems are responding to environmental changes, but the causes of individual migratory behaviours have proven difficult to isolate. We show that, in a partially migratory population of Eurasian oystercatchers (Haematopus ostralegus), the migratory behaviour of progeny follows paternal but not maternal behaviour, and is unrelated to timing of hatching or fledging. These findings highlight the key role of social interactions in shaping the migratory behaviour of new generations, and thus the spatio-temporal distribution of migratory populations.
No echo in the chambers of political interactions on Reddit
Authors: De Francisci Morales Gianmarco , Monti Corrado , Starnini Michele
Published: February 2021

Echo chambers in online social networks, whereby users’ beliefs are reinforced by interactions with like-minded peers and insulation from others’ points of view, have been decried as a cause of political polarization. Here, we investigate their role in the debate around the 2016 US elections on Reddit, a fundamental platform for the success of Donald Trump. We identify Trump vs Clinton supporters and reconstruct their political interaction network. We observe a preference for cross-cutting political interactions between the two communities rather than within-group interactions, thus contradicting the echo chamber narrative. Furthermore, these interactions are asymmetrical: Clinton supporters are particularly eager to answer comments by Trump supporters. Beside asymmetric heterophily, users show assortative behavior for activity, and disassortative, asymmetric behavior for popularity. Our findings are tested against a null model of random interactions, by using two different approaches: a network rewiring which preserves the activity of nodes, and a logit regression which takes into account possible confounding factors. Finally, we explore possible socio-demographic implications. Users show a tendency for geographical homophily and a small positive correlation between cross-interactions and voter abstention. Our findings shed light on public opinion formation on social media, calling for a better understanding of the social dynamics at play in this context.
Environmental (e)RNA advances the reliability of eDNA by predicting its age
Authors: Marshall Nathaniel T. , Vanderploeg Henry A. , Chaganti Subba Rao
Published: February 2021

Environmental DNA (eDNA) analysis has advanced conservation biology and biodiversity management. However, accurate estimation of age and origin of eDNA is complicated by particle transport and the presence of legacy genetic material, which can obscure accurate interpretation of eDNA detection and quantification. To understand the state of genomic material within the environment, we investigated the degradation relationships between (a) size of fragments (long vs short), (b) genomic origins (mitochondrial vs nuclear), (c) nucleic acids (eDNA vs eRNA), and (d) RNA types (messenger (m)RNA vs ribosomal (r)RNA) from non-indigenous Dreissena mussels. Initial concentrations of eRNA followed expected transcriptional trends, with rRNAs found at > 1000 × that of eDNA, and a mitosis-associated mRNA falling below detection limits within 24 h. Furthermore, the ratio of eRNA:eDNA significantly decreased throughout degradation, potentially providing an estimate for the age of genomic material. Thus, eRNA quantification can increase detection due to the high concentrations of rRNAs. Furthermore, it may improve interpretation of positive detections through the eRNA:eDNA ratio and/or by detecting low abundant mitosis-associated mRNAs that degrade within ~ 24 h.
High-fructose feeding does not induce steatosis or non-alcoholic fatty liver disease in pigs
Authors: Schmidt Nikolaj H. , Svendsen Pia , Albarrán-Juárez Julián , Moestrup Søren K. , Bentzon Jacob Fog
Published: February 2021

Non-alcoholic fatty liver disease (NAFLD) is an increasingly prevalent condition that has been linked to high-fructose corn syrup consumption with induction of hepatic de novo lipogenesis (DNL) as the suggested central mechanism. Feeding diets very high in fructose (> 60%) rapidly induce several features of NAFLD in rodents, but similar diets have not yet been applied in larger animals, such as pigs. With the aim to develop a large animal NAFLD model, we analysed the effects of feeding a high-fructose (HF, 60% w/w) diet for four weeks to castrated male Danish Landrace-York-Duroc pigs. HF feeding upregulated expression of hepatic DNL proteins, but levels were low compared with adipose tissue. No steatosis or hepatocellular ballooning was seen on histopathological examination, and plasma levels of transaminases were similar between groups. Inflammatory infiltrates and the amount of connective tissue was slightly elevated in liver sections from fructose-fed pigs, which was corroborated by up-regulation of macrophage marker expression in liver homogenates. Supported by RNA-profiling, quantitative protein analysis, histopathological examination, and biochemistry, our data suggest that pigs, contrary to rodents and humans, are protected against fructose-induced steatosis by relying on adipose tissue rather than liver for DNL.
Panel of serum miRNAs as potential non-invasive biomarkers for pancreatic ductal adenocarcinoma
Authors: Khan Imteyaz Ahmad , Rashid Safoora , Singh Nidhi , Rashid Sumaira , Singh Vishwajeet , Gunjan Deepak , Das Prasenjit , Dash Nihar Ranjan , Pandey Ravindra Mohan , Chauhan Shyam Singh , Gupta Surabhi , Saraya Anoop
Published: February 2021

Early-stage diagnosis of pancreatic ductal adenocarcinoma (PDAC) is difficult due to non-specific symptoms. Circulating miRNAs in body fluids have been emerging as potential non-invasive biomarkers for diagnosis of many cancers. Thus, this study aimed to assess a panel of miRNAs for their ability to differentiate PDAC from chronic pancreatitis (CP), a benign inflammatory condition of the pancreas. Next-generation sequencing was performed to identify miRNAs present in 60 FFPE tissue samples (27 PDAC, 23 CP and 10 normal pancreatic tissues). Four up-regulated miRNAs (miR-215-5p, miR-122-5p, miR-192-5p, and miR-181a-2-3p) and four down-regulated miRNAs (miR-30b-5p, miR-216b-5p, miR-320b, and miR-214-5p) in PDAC compared to CP were selected based on next-generation sequencing results. The levels of these 8 differentially expressed miRNAs were measured by qRT-PCR in 125 serum samples (50 PDAC, 50 CP, and 25 healthy controls (HC)). The results showed significant upregulation of miR-215-5p, miR-122-5p, and miR-192-5p in PDAC serum samples. In contrast, levels of miR-30b-5p and miR-320b were significantly lower in PDAC as compared to CP and HC. ROC analysis showed that these 5 miRNAs can distinguish PDAC from both CP and HC. Hence, this panel can serve as a non-invasive biomarker for the early detection of PDAC.
Correlation minor norms, entanglement detection and discord
Authors: Peled Bar Y. , Te’eni Amit , Carmi Avishy , Cohen Eliahu
Published: February 2021

In this paper we develop an approach for detecting entanglement, which is based on measuring quantum correlations and constructing a correlation matrix. The correlation matrix is then used for defining a family of parameters, named Correlation Minor Norms, which allow one to detect entanglement. This approach generalizes the computable cross-norm or realignment (CCNR) criterion, and moreover requires measuring a state-independent set of operators. Furthermore, we illustrate a scheme which yields for each Correlation Minor Norm a separable state that maximizes it. The proposed entanglement detection scheme is believed to be advantageous in comparison to other methods because correlations have a simple, intuitive meaning and in addition they can be directly measured in experiment. Moreover, it is demonstrated to be stronger than the CCNR criterion. We also illustrate the relation between the Correlation Minor Norm and entanglement entropy for pure states. Finally, we discuss the relation between the Correlation Minor Norm and quantum discord. We demonstrate that the CMN may be used to define a new measure for quantum discord.
A synthetic kinematic index of trunk displacement conveying the overall motor condition in Parkinson’s disease
Authors: Troisi Lopez Emahnuel , Minino Roberta , Sorrentino Pierpaolo , Rucco Rosaria , Carotenuto Anna , Agosti Valeria , Tafuri Domenico , Manzo Valentino , Liparoti Marianna , Sorrentino Giuseppe
Published: February 2021

Parkinson's disease (PD) is characterized by motor impairment, affecting quality of life and increasing fall risk, due to ineffective postural control. To this day, the diagnosis remains based on clinical approach. Similarly, motor evaluation is based on heterogeneous, operator-dependent observational criteria. A synthetic, replicable index to quantify motor impairment is still lacking. Hence, we have designed a new measure of postural stability which assesses the trunk displacement in relation to the center of mass, that we named trunk displacement index (TDI). Twenty-three PD patients and twenty-three healthy controls underwent motor examination through a stereophotogrammetric system. A correlation analysis was performed to assess the relationship of TDI with gait parameters and clinical motor scale (UPDRS-III). The TDI sensitivity was estimated, comparing pre- and post- L-DOPA subclinical dose intake. The TDI showed significant correlations with many gait parameters and with the UPDRS-III. Furthermore, the TDI resulted capable in discriminating between off and on state in PD, whereas gait parameters failed two show any difference between those two conditions. Our results suggest that the TDI may be considered a highly sensitive biomechanical index, reflecting the overall motor condition in PD, and provided of clinical relevance due to the correlation with the clinical evaluation.
Using stable isotopes as tracer to investigate hydrological condition and estimate water residence time in a plain region, Chengdu, China
Authors: Zhou Jing , Liu Guodong , Meng Yuchuan , Xia ChengCheng , Chen Ke , Chen Yu
Published: February 2021

The oxygen and hydrogen isotopic compositions (δ18O and δ2H) were measured on river water and precipitation collected from four sub-catchments within the upper Tuojiang River catchment. δ18O values of river water and precipitation exhibit significant seasonal variations. These seasonal variations are used for estimating the mean residence time (MRT) for four sub-catchments by an exponential model, ranging from 346 to 493 days. The correlation between catchment MRT and mean slope of the catchment (r2 = 0.29) is weak, while the correlations between catchment MRT, catchment area (r2 = 0.79) and topographic index (r2 = 0.98) are strong. These results indicate that topography and catchment area, both control the catchment MRT and the topographic index may be a reliable parameter for estimating the catchment MRT. Moreover, the relationship between land use types and MRT was investigated. The results show that paddy fields (r2 = 0.95) compared to the other land use types may have a greater impact on the MRT of the irrigation-dominated catchment. This study provides a preliminary exploration of the factors affecting MRT in the plain region and a basis for simulating MRT in the future.
Dufour’s gland analysis reveals caste and physiology specific signals in Bombus impatiens
Authors: Derstine Nathan T. , Villar Gabriel , Orlova Margarita , Hefetz Abraham , Millar Jocelyn , Amsalem Etya
Published: February 2021

Reproductive division of labor in insect societies is regulated through multiple concurrent mechanisms, primarily chemical and behavioral. Here, we examined if the Dufour’s gland secretion in the primitively eusocial bumble bee Bombus impatiens signals information about caste, social condition, and reproductive status. We chemically analyzed Dufour’s gland contents across castes, age groups, social and reproductive conditions, and examined worker behavioral and antennal responses to gland extracts. We found that workers and queens each possess caste-specific compounds in their Dufour’s glands. Queens and gynes differed from workers based on the presence of diterpene compounds which were absent in workers, whereas four esters were exclusive to workers. These esters, as well as the total amounts of hydrocarbons in the gland, provided a separation between castes and also between fertile and sterile workers. Olfactometer bioassays demonstrated attraction of workers to Dufour’s gland extracts that did not represent a reproductive conflict, while electroantennogram recordings showed higher overall antennal sensitivity in queenless workers. Our results demonstrate that compounds in the Dufour’s gland act as caste- and physiology-specific signals and are used by workers to discriminate between workers of different social and reproductive status.
Transcriptomic uniqueness and commonality of the ion channels and transporters in the four heart chambers
Authors: Iacobas Sanda , Amuzescu Bogdan , Iacobas Dumitru A.
Published: February 2021

Myocardium transcriptomes of left and right atria and ventricles from four adult male C57Bl/6j mice were profiled with Agilent microarrays to identify the differences responsible for the distinct functional roles of the four heart chambers. Female mice were not investigated owing to their transcriptome dependence on the estrous cycle phase. Out of the quantified 16,886 unigenes, 15.76% on the left side and 16.5% on the right side exhibited differential expression between the atrium and the ventricle, while 5.8% of genes were differently expressed between the two atria and only 1.2% between the two ventricles. The study revealed also chamber differences in gene expression control and coordination. We analyzed ion channels and transporters, and genes within the cardiac muscle contraction, oxidative phosphorylation, glycolysis/gluconeogenesis, calcium and adrenergic signaling pathways. Interestingly, while expression of Ank2 oscillates in phase with all 27 quantified binding partners in the left ventricle, the percentage of in-phase oscillating partners of Ank2 is 15% and 37% in the left and right atria and 74% in the right ventricle. The analysis indicated high interventricular synchrony of the ion channels expressions and the substantially lower synchrony between the two atria and between the atrium and the ventricle from the same side.
Exogenous hemin improves Cd2+ tolerance and remediation potential in Vigna radiata by intensifying the HO-1 mediated antioxidant defence system
Authors: Mahawar Lovely , Popek Robert , Shekhawat Gyan Singh , Alyemeni Mohammed Nasser , Ahmad Parvaiz
Published: February 2021

The present study evaluated the effects of exogenous hemin on cadmium toxicity in terms of metal accretion and stress resilience in Vigna radiata L. (Wilczek). One-week-old seedlings were treated with CdCl2 (50 μM) alone and in combination with hemin (0.5 mM) in half-strength Hoagland medium for 96 h. The optimum concentrations of Cd and hemin were determined on the basis of haem oxygenase-1 activity. The results demonstrated that under Cd stress, plants accumulated a considerable amount of metal in their tissues, and the accumulation was higher in roots than in leaves, which significantly reduced the plant biomass and chlorophyll content by increasing the oxidative stress (MDA and H2O2 content). However, hemin supplementation under Cd,-stress improved plant growth by enhancing the harvestable biomass and photosynthetic pigments, increasing antioxidant activities (SOD, APX, POD, HO-1 and proline), lowering oxidative damage and increasing Cd tolerance in plants. Furthermore, the application of hemin enhances the removal efficiency of Cd in V. radiata by increasing the uptake of Cd via roots and its translocation from roots to foliar tissues. Thus, the study suggests that hemin has the potential to improve the stress tolerance and phytoremediation ability of heavy metal-tolerant plants so that they can be used instead of hyperaccumulators for remediation of Cd-contaminated environments.
Tunable electronic structure and magnetic anisotropy in bilayer ferromagnetic semiconductor Cr2Ge2Te6
Authors: Ren Wen-ning , Jin Kui-juan , Wang Jie-su , Ge Chen , Guo Er-Jia , Ma Cheng , Wang Can , Xu Xiulai
Published: February 2021

The emergence of ferromagnetism in two-dimensional van der Waals materials has aroused broad interest. However, the ferromagnetic instability has been a problem remained. In this work, by using the first-principles calculations, we identified the critical ranges of strain and doping for the bilayer Cr2Ge2Te6 within which the ferromagnetic stability can be enhanced. Beyond the critical range, the tensile strain can induce the phase transition from the ferromagnetic to the antiferromagnetic, and the direction of magnetic easy axis can be converted from out-of-plane to in-plane due to the increase of compressive strain, or electrostatic doping. We also predicted an electron doping range, within which the ferromagnetism can be enhanced, while the ferromagnetic stability was maintained. Moreover, we found that the compressive strain can reverse the spin polarization of electrons at the conduction band minimum, so that two categories of half-metal can be induced by controlling electrostatic doping in the bilayer Cr2Ge2Te6. These results should shed a light on achieving ferromagnetic stability for low-dimensional materials.
Molecular profiling of epigenetic landscape of cancer cells during extracellular matrix detachment
Authors: Khan Mohammad Imran , Zamzami Mazin A. , Ahmad Aftab , Choudhry Hani
Published: February 2021

During cancer, a major challenge faced by oncologists is the treatment of metastasis; a leading cause of cancer-related deaths around the world. Metastasis involves a highly ordered sequence of events starting with the detachment of tumor cells from the extracellular matrix (E.C.M.). In normal cells, detachment from E.C.M. triggers programmed cell death, termed anoikis. However, tumor cells dodge their way to anoikis and spread to distant sites for initiating the metastatic program. In this work, we explored the impact of E.C.M. detachment on the expression of some major oncogenic histone methyltransferases. Results showed both EZH2 expression and its enzymatic activity were significantly increased in E.C.M. detached cancer cells when compared to the attached cells. Inhibition of EZH2 results in a significant reduction in cell proliferation, spheroids size, and induction in apoptosis in E.C.M. detached cells. Furthermore, we observed a reduction in EZH2 expression levels in single cells when compared to clusters of E.C.M. detached cells. Finally, we combined the EZH2 inhibition with AMPK, known to be highly expressed in E.C.M. detached cancer cells and observed antagonistic effects between the two pathways. The observed results clearly showed that E.C.M. detached cancer cells require oncogenic EZH2 and can be targeted by EZH2 inhibitors.
Differentiating malignant from benign salivary gland lesions: a multiparametric non-contrast MR imaging approach
Authors: Takumi Koji , Nagano Hiroaki , Kikuno Hidehiko , Kumagae Yuichi , Fukukura Yoshihiko , Yoshiura Takashi
Published: February 2021

The purpose of this study is to determine whether multiparametric non-contrast MR imaging including diffusion-weighted imaging (DWI), arterial spin labeling (ASL), and amide proton transfer (APT) weighted imaging can help differentiate malignant from benign salivary gland lesions. The study population consisted of 42 patients, with 31 benign and 11 malignant salivary gland lesions. All patients were evaluated using DWI, three-dimensional pseudo-continuous ASL, and APT-weighted imaging on 3 T MR imaging before treatment. Apparent diffusion coefficient (ADC), tumor blood flow (TBF), and APT-related signal intensity (APTSI) values within the lesion were compared between the malignant and benign lesions by Mann–Whitney U test. For each parameter, optimal cutoff values were chosen using a threshold criterion that maximized the Youden index for predicting malignant lesions. The performance of ADC, TBF, APTSI, individually and combined, was evaluated in terms of diagnostic ability for malignant lesions. Diagnostic performance was compared by McNemar test. APTSI was significantly higher in malignant lesions (2.18 ± 0.89%) than in benign lesions (1.57 ± 1.09%, p = 0.047). There was no significant difference in ADC or TBF between benign and malignant lesions (p = 0.155 and 0.498, respectively). The accuracy of ADC, TBF, and APTSI for diagnosing malignant lesions was 47.6%, 50.0%, and 66.7%, respectively; whereas the accuracy of the three parameters combined was 85.7%, which was significantly higher than that of each parameter alone (p = 0.001, 0.001, and 0.008, respectively). Therefore, the combination of ADC, TBF, and APTSI can help differentiate malignant from benign salivary gland lesions.
Forging a Bayesian link between habitat selection and avoidance behavior in a grassland grouse
Authors: Patten Michael A. , Barnard Alexandra A. , Curry Claire M. , Dang Henry , Loraamm Rebecca W.
Published: February 2021

Habitat selection is a basic aspect of the ecology of many species, yet often the term is conflated or confused with both habitat preference and habitat use. We argue that each term fits within a conceptual framework that can be viewed in Bayesian terms and demonstrate, using long-term data on occupancy patterns of a grassland grouse, how prior probability profiles can be estimated. We obtained estimates by specifically focusing on whether and to what extent the Lesser Prairie-Chicken (Tympanuchus pallidicinctus) avoids anthropogenic features such as roads, powerlines, petroleum wells, fences, and buildings, in two study areas, one with denser and one with sparser incidence of features. Grouse strongly avoided large features such as outbuildings and tended to avoid tall features such as powerlines; by contrast, grouse did not or only slightly avoided low, unobtrusive features such as fences. We further examined co-location of pairs of anthropogenic features and found that certain features were avoided so strongly that avoidance distance may be shorter for other features; that is, birds were “pushed toward” some features because they are “pushed away” from others. In each case, our approach points toward a means to incorporate avoidance behavior directly into analytic studies of habitat selection, in that data on use (the posterior, as it were) could be used to infer the selection process provided data on preference (the prior, as it were) could be obtained.
Alterations in the gut bacterial microbiome in people with type 2 diabetes mellitus and diabetic retinopathy
Authors: Das Taraprasad , Jayasudha Rajagopalaboopathi , Chakravarthy SamaKalyana , Prashanthi Gumpili Sai , Bhargava Archana , Tyagi Mudit , Rani Padmaja Kumari , Pappuru Rajeev Reddy , Sharma Savitri , Shivaji Sisinthy
Published: February 2021

Gut bacterial microbiome dysbiosis in type 2 Diabetes Mellitus (T2DM) has been reported, but such an association with Diabetic Retinopathy (DR) is not known. We explored possible link between gut bacterial microbiome dysbiosis and DR. Using fecal samples of healthy controls (HC) and people with T2DM with/without DR, gut bacterial communities were analysed using 16S rRNA gene sequencing and data analysed using QIIME and R software. Dysbiosis in the gut microbiomes, at phyla and genera level, was observed in people with T2DM and DR compared to HC. People with DR exhibited greater discrimination from HC. Microbiomes of people with T2DM and DR were also significantly different. Both DM and DR microbiomes showed a decrease in anti-inflammatory, probiotic and other bacteria that could be pathogenic, compared to HC, and the observed change was more pronounced in people with DR. This is the first report demonstrating dysbiosis in the gut microbiome (alteration in the diversity and abundance at the phyla and genera level) in people with DR compared to HC. Such studies would help in developing novel and targeted therapies to improve treatment of DR.
Potentilla anserina L. developmental changes affect the rhizosphere prokaryotic community
Authors: Wang Yaqiong , Liu Yuxi , Li Xue , Han Xiaoyan , Zhang Zhen , Ma Xiaoling , Li Junqiao
Published: February 2021

Plant roots and soil prokaryotes primarily interact with each other in the rhizosphere. Changes in the rhizosphere prokaryotic structure are influenced by several factors. In this study, the community structure of the Potentilla anserina L. rhizosphere prokaryotes was identified and evaluated by high-throughput sequencing technology in different continuous cropping fields and developmental stages of the plant. In total, 2 archaeal (Euryarchaeota and Thaumarchaeota) and 26 bacterial phyla were identified in the P. anserina rhizosphere. The bacterial community was mainly composed of Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Gemmatimonadetes, Planctomycetes, Proteobacteria, and Verrucomicrobia. Moreover, the prokaryotic community structure of the rhizosphere varied significantly during plant development. Our results provide new insights into the dynamics of the P. anserina rhizosphere prokaryotic community and may provide useful information for enhancing the growth and development of P. anserina through artificial control of the soil prokaryotes.
Importance of diagnosis-to-treatment interval in newly diagnosed patients with diffuse large B-cell lymphoma
Authors: Yoshida Masahiro , Nakaya Yosuke , Shimizu Katsujun , Tatsumi Naoko , Tsutsumi Minako , Fuseya Hoyuri , Horiuchi Mirei , Yoshimura Takuro , Hayashi Yoshiki , Nakao Takafumi , Inoue Takeshi , Yamane Takahisa
Published: February 2021

Treatment of patients with malignancy sometimes be delayed due to various reasons. Several studies revealed that an influence of diagnosis-to-treatment interval (DTI) on outcomes differs depending on the type of malignancy. In this study, we evaluated the influence of DTI on clinical outcomes in newly diagnosed patients with diffuse large B-cell lymphoma (DLBCL). A total of 199 patients were identified with a median DTI of 22 days. At 2 years, patients with short DTI (0–22 days) showed significantly poorer OS (62.7% vs 86.4%) and PFS (55.1% vs 75.9%) compared to those with long DTI (over 22 days). Although short DTI was strongly correlated with several known adverse factors, it remained to be an independent prognostic factor by multivariate analysis. In conclusion, our study confirmed the importance of DTI in patients with DLBCL. Researchers should consider DTI as one of the important prognostic factors and plan clinical trials to be able to enroll patients with aggressive disease requiring urgent treatment.
Circulatory shear stress induces molecular changes and side population enrichment in primary tumor-derived lung cancer cells with higher metastatic potential
Authors: Alvarado-Estrada Keila , Marenco-Hillembrand Lina , Maharjan Sushila , Mainardi Valerio Luca , Zhang Yu Shrike , Zarco Natanael , Schiapparelli Paula , Guerrero-Cazares Hugo , Sarabia-Estrada Rachel , Quinones-Hinojosa Alfredo , Chaichana Kaisorn L.
Published: February 2021

Cancer is a leading cause of death and disease worldwide. However, while the survival for patients with primary cancers is improving, the ability to prevent metastatic cancer has not. Once patients develop metastases, their prognosis is dismal. A critical step in metastasis is the transit of cancer cells in the circulatory system. In this hostile microenvironment, variations in pressure and flow can change cellular behavior. However, the effects that circulation has on cancer cells and the metastatic process remain unclear. To further understand this process, we engineered a closed-loop fluidic system to analyze molecular changes induced by variations in flow rate and pressure on primary tumor-derived lung adenocarcinoma cells. We found that cancer cells overexpress epithelial-to-mesenchymal transition markers TWIST1 and SNAI2, as well as stem-like marker CD44 (but not CD133, SOX2 and/or NANOG). Moreover, these cells display a fourfold increased percentage of side population cells and have an increased propensity for migration. In vivo, surviving circulatory cells lead to decreased survival in rodents. These results suggest that cancer cells that express a specific circulatory transition phenotype and are enriched in side population cells are able to survive prolonged circulatory stress and lead to increased metastatic disease and shorter survival.
LIMPID: a versatile method for visualization of brain vascular networks
Authors: Xie Wenguang , Gong Xiao-Ting , Cheng Xiaofeng , Cao Jingjing , Zhao Jin , Zhang Hao-Li , Zhang Shengxiang
Published: February 2021

Amino-functionalized and fluorescence-labeled nanoparticles were used to label blood vessels in the brain. This method achieved stable labeling of cerebrovascular networks and was compatible with tissue clearing and multi-channel imaging.
Dynamic change of MMP-9 in diabetic stroke visualized by optical imaging and treated with CD28 superagonist
Authors: Cai Yu , Leng Shou , Ma Yuanyuan , Xu Tingting , Chang Di , Ju Shenghong
Published: February 2021

By utilizing NIRF imaging, diabetic stroke mice were visualized with a trend of higher levels of MMP-9 than wild-type mice. CD28 SA down-regulated the expression of MMP-9 and represents a potential treatment to diabetic stroke.