bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Noni (Morinda citrifolia L.) Fruit Extract Supplemented in Cashmere Goats with a High-Concentrate Diet on Growth Performance, Ruminal and Colonic Fermentation and SARA
Authors: Zhang Qingyue , Dong Shuhui , Yu Hao , Li Yinhao , Guo Xiaoyu , Zhao Yanli , Guo Yongmei , Yan Sumei
Published: October 2023

This experiment was conducted to investigate the effects of noni fruit extract (NFE) on growth performance, ruminal and colonic fermentation, nutrient digestion, and subacute rumen acidosis (SARA) of cashmere goats with the high-concentrate diet. Twenty-four cashmere kids (17.9 ± 1.45 kg of BW ± SD) were randomly assigned to three treatments: low-concentrate diet, high-concentrate (HC) diet, or HC diet supplemented with NFE at 1 g per kg DM (0.1%). The results showed that although the HC diet improved the average daily gain (ADG) and feed conversion rate (FCR), it was accompanied by SARA with a decreased pH and an increased lactic acid of both rumen and colon, and decreased digestibility of neutral detergent fiber (NDF)and acid detergent fiber (ADF). The supplementation of 0.10% NFE in the HC diet could not only effectively alleviate SARA symptoms and colon fermentation disorders, such as reversing the decrease of pH and alleviating the increase of lactic acid in rumen and colon, but also mitigate the decline of fiber digestibility caused by long-term feeding in the HC diet, and increase the digestibility of crude protein(CP) and dry matter (DM), which improved the ADG and FCR of cashmere kids. Thus, NFE provides new strategies for alleviating SARA and promoting cashmere goat growth.
Effects of Genetic Origin of Honeybees and Climate on Prevalence and Infestation Levels of Varroa
Authors: García-Figueroa Claudia , Ramírez-Ramírez Francisco Javier , Alvarado-Avila Laura Yavarik , Arechavaleta-Velasco Miguel Enrique
Published: October 2023

The objective of this study was to evaluate the effect of honeybee genetic origin, climate type and the interactions between these variables on the prevalence and infestation levels of Varroa in a large population of honeybee colonies (n = 1134). For each colony, the morphotype, haplotype and climate type were determined. No differences between the Africanized, European and Hybrid morphotypes were found for the prevalence and infestation levels of Varroa (p > 0.05). Differences between honeybee haplotypes were found for the prevalence of Varroa (p < 0.05), and the prevalence was higher in the African haplotype than in the European haplotype. No differences between honeybee haplotypes were found for the infestation levels of Varroa (p > 0.05). Differences were found between climate type for the prevalence and infestation levels of Varroa (p < 0.05): the temperate sub-humid climate had a higher prevalence and higher infestation levels than the semi-warm climate and the warm sub-humid climate. Correlations between the infestation levels of Varroa and mean annual temperature, mean annual precipitation, winter precipitation and Lang index were found.
Combination of Cysteine and Glutathione Prevents Ethanol-Induced Hangover and Liver Damage by Modulation of Nrf2 Signaling in HepG2 Cells and Mice
Authors: Kim Hyeongyeong , Suh Hyung Joo , Hong Ki-Bae , Jung Eun-Jin , Ahn Yejin
Published: October 2023

Excessive alcohol consumption increases oxidative stress, leading to alcoholic liver disease. In this study, the protective effects of a mixture of cysteine and glutathione against ethanol-induced hangover and liver damage were evaluated in mice and HepG2 cells. Ethanol (2 mL/kg) was orally administered to the mice 30 min before receiving the test compounds (200 mg/kg), and the behavioral and oxidative stress-related biochemical parameters altered by ethanol were analyzed. Acute ethanol administration increased anxiety behavior and decreased balance coordination in mice (p < 0.001); however, a mixture of cysteine and glutathione (MIX) in a 3:1 ratio improved alcohol-induced behavior more effectively than the individual compounds (p < 0.001). The MIX group showed higher ethanol-metabolizing enzyme activity than the control group (p < 0.001) and significantly suppressed the elevation of serum alcohol (p < 0.01) and acetaldehyde (p < 0.001) levels after 1 h of ethanol administration. In HepG2 cells, 2.5 mM MIX accelerated ethanol metabolism and reduced cytochrome P450 2E1 mRNA expression (p < 0.001). MIX also increased the expression of antioxidant enzymes through the upregulation of nuclear erythroid 2-related factor 2 (Nrf2) signaling and consequently suppressed the overproduction of reactive oxygen species and malondialdehyde (p < 0.001). Collectively, MIX alleviates the hangover symptoms and attenuates the alcohol-induced oxidative stress by regulating the Nrf2 pathway.
Effects of Acetyl-L-Carnitine on Oxidative Stress in Amyotrophic Lateral Sclerosis Patients: Evaluation on Plasma Markers and Members of the Neurovascular Unit
Authors: Grossini Elena , De Marchi Fabiola , Venkatesan Sakthipriyan , Mele Angelica , Ferrante Daniela , Mazzini Letizia
Published: October 2023

Oxidative stress, the alteration of mitochondrial function, and the neurovascular unit (NVU), play a role in Amyotrophic Lateral Sclerosis (ALS) pathogenesis. We aimed to demonstrate the changes in the plasma redox system and nitric oxide (NO) in 32 new ALS-diagnosed patients in treatment with Acetyl-L-Carnitine (ALCAR) compared to healthy controls. We also evaluated the effects of plasma on human umbilical cord-derived endothelial vascular cells (HUVEC) and astrocytes. The analyses were performed at the baseline (T0), after three months (T1), and after six months (T2). In ALS patients at T0/T1, the plasma markers of lipid peroxidation, thiobarbituric acid reactive substances (TBARS) and 4-hydroxy nonenal (4-HNE) were higher, whereas the antioxidants, glutathione (GSH) and the glutathione peroxidase (GPx) activity were lower than in healthy controls. At T2, plasma TBARS and 4-HNE decreased, whereas plasma GSH and the GPx activity increased in ALS patients. As regards NO, the plasma levels were firmly lower at T0–T2 than those of healthy controls. Cell viability, and mitochondrial membrane potential in HUVEC/astrocytes treated with the plasma of ALS patients at T0–T2 were reduced, while the oxidant release increased. Those results, which confirmed the fundamental role of oxidative stress, mitochondrial function, and of the NVU in ALS pathogenesis, can have a double meaning, acting as disease markers at baseline and potential markers of drug effects in clinical practice and during clinical trials.
Impaired Vestibulo-Ocular Reflex on Video Head Impulse Test in Superior Canal Dehiscence: “Spontaneous Plugging” or Endolymphatic Flow Dissipation?
Authors: Castellucci Andrea , Malara Pasquale , Martellucci Salvatore , Alfarghal Mohamad , Brandolini Cristina , Piras Gianluca , Armato Enrico , Ruberto Rosanna Rita , Brizzi Pasquale , Presutti Livio , Ghidini Angelo
Published: October 2023

Surgical plugging of the superior semicircular canal (SSC) represents an effective procedure to treat disabling symptoms in superior canal dehiscence (SCD), despite resulting in an impaired vestibulo-ocular reflex (VOR) gain for the SSC. On the other hand, SSC hypofunction on video head impulse test (vHIT) represents a common finding in patients with SCD exhibiting sound/pressure-induced vertigo, a low-frequency air–bone gap (ABG), and enhanced vestibular-evoked myogenic potentials (VEMPs). “Spontaneous canal plugging” has been assumed as the underlying process. Nevertheless, missing/mitigated symptoms and/or near-normal instrumental findings would be expected. An endolymphatic flow dissipation has been recently proposed as an alternative pathomechanism for SSC VOR gain reduction in SCD. We aimed to shed light on this debate by comparing instrumental findings from 46 ears of 44 patients with SCD exhibiting SSC hypofunction with post-operative data from 10 ears of 10 patients with SCD who underwent surgical plugging. While no difference in SSC VOR gain values was found between the two groups (p = 0.199), operated ears developed a posterior canal hypofunction (p = 0.002). Moreover, both ABG values (p = 0.012) and cervical/ocular VEMP amplitudes (p < 0.001) were significantly higher and VEMP thresholds were significantly lower (p < 0.001) in ears with SCD compared to operated ears. According to our data, canal VOR gain reduction in SCD should be considered as an additional sign of a third window mechanism, likely due to an endolymphatic flow dissipation.
Animal Models in Diabetic Research—History, Presence, and Future Perspectives
Authors: Pandey Shashank , Chmelir Tomas , Chottova Dvorakova Magdalena
Published: October 2023

Diabetes mellitus (DM) is a very serious disease, the incidence of which has been increasing worldwide. The beginning of diabetic research can be traced back to the 17th century. Since then, animals have been experimented on for diabetic research. However, the greatest development of diabetes research occurred in the second half of the last century, along with the development of laboratory techniques. Information obtained by monitoring patients and animal models led to the finding that there are several types of DM that differ significantly from each other in the causes of the onset and course of the disease. Through different types of animal models, researchers have studied the pathophysiology of all types of diabetic conditions and discovered suitable methods for therapy. Interestingly, despite the unquestionable success in understanding DM through animal models, we did not fully succeed in transferring the data obtained from animal models to human clinical research. On the contrary, we have observed that the chances of drug failure in human clinical trials are very high. In this review, we will summarize the history and presence of animal models in the research of DM over the last hundred years. Furthermore, we have summarized the new methodological approaches, such as “organ-on-chip,” that have the potential to screen the newly discovered drugs for human clinical trials and advance the level of knowledge about diabetes, as well as its therapy, towards a personalized approach.
Changes in Tinnitus Characteristics and Residual Inhibition following Cochlear Implantation: A Prospective Analysis
Authors: Deklerck Ann Nancy , Swinnen Freya , Keppler Hannah , Dhooge Ingeborg Johanna Maria
Published: October 2023

This study aims to explore the effect of cochlear implantation on tinnitus perception. A prospective study was conducted on 72 adult hearing-impaired patients to evaluate tinnitus perception before and after cochlear implantation, using standardized tinnitus questionnaires (the tinnitus sample case-history questionnaire, tinnitus functional index (TFI), and tinnitus handicap inventory (THI)). A large variety of demographic and hearing- and implant-related data was collected from patient hospital records to explore possible associations with the implantation effect. The prevalence of tinnitus complaints before implantation was 58.3%. The temporary induction or aggravation of tinnitus immediately after surgery was noted in 20% and 46.7% of patients, respectively. When evaluated 3 months after implantation, 60% of tinnitus patients experienced a clinically significant reduction in their complaints; most of the improvements were experienced immediately after activation of the implant. Only the scores for TFI and THI at baseline were found to be significantly correlated with a reduction in TFI scores after implantation. In 80% of tinnitus patients, the tinnitus remained suppressed for some time after taking off the device. The large subset of patients with residual inhibition supports the involvement of central pathophysiological processes in implantation effects on tinnitus, which are explored in this paper.
Dispelling Mist That Obscures Positional Vertigo in Vestibular Migraine
Authors: Tian E , Li Fei , Liu Dan , Wang Jun , Guo Zhaoqi , Chen Jingyu , Guo Jiaqi , Zhang Sulin
Published: October 2023

(1) Background: Patients with vestibular migraine (VM) often present with positional vertigo. A portion of these patients have features of benign paroxysmal positional vertigo (BPPV). It is a challenge to rapidly identify the BPPV component of VM associated with positional vertigo. (2) Methods: Retrospective data collected from 60 VM and 47 VM + BPPV patients were used to build a diagnostic model, and then prospective data from 47 patients were used for the external validation. All patients had VM manifesting as positional vertigo, with or without accompanying BPPV. The clinical manifestations and the results of vestibular function tests were comprehensively analyzed using logistic regression. (3) Results: The univariate and multivariate analyses showed that the age, symptom duration, tinnitus, ear fullness, nausea, head shaking nystagmus, the direction of the Dix–Hallpike and roll tests, and horizontal gain could help differentiate between the two groups. A nomogram and an online calculator were generated. The C-index was 0.870. The diagnostic model showed good discriminative power and calibration performance during internal and external validation. (4) Conclusions: This study provided a new perspective for diagnosing VM with positional vertigo by identifying the BPPV component and, for the first time, offers a prediction model integrating multiple predictors.
Examining the Effects of Theory of Mind and Social Skills Training on Social Competence in Adolescents with Autism
Authors: Ma Weina , Mao Jieyu , Xie Yu , Li Simeng , Wang Mian
Published: October 2023

Individuals with autism spectrum disorders (ASD) have impairment in interpreting emotional communication and the mental states of others, which limits their social competence. Mounting evidence has suggested that theory of mind (ToM) is a vital strategy to enhance social communication and interaction skills of children with ASD. However, very little research has looked at how ToM and social skills training affect social competence in adolescents with autism. This study examined the effectiveness of an intervention program, ToM-SS, which integrated the ToM and social skills training to improve the social competence of three adolescents with autism. A multiple baseline across behaviors design was adopted to evaluate the participants’ learning outcomes and demonstrated a functional relationship between intervention and skill mastery. Results show that the intervention produced substantial improvements in students’ acquisition of ToM (e.g., seeing leads to knowing and identifying desire-based and context-based emotions) and targeted social skills (e.g., praising others, expressing emotion and seeking help). Feedback and comments from teachers and parents also indicate good social validity of the intervention program.
Validity of Galerkin Method at Beam’s Nonlinear Vibrations of the Single Mode with the Initial Curvature
Authors: Zhang Yunbo , Huang Kun , Xu Wei
Published: October 2023

A common strategy for studying the nonlinear vibrations of beams is to discretize the nonlinear partial differential equation into a nonlinear ordinary differential equation or equations through the Galerkin method. Then, the oscillations of beams are explored by solving the ordinary differential equation or equations. However, recent studies have shown that this strategy may lead to erroneous results in some cases. The present paper carried out the following three research studies: (1) We performed Galerkin first-order and second-order truncations to discrete the nonlinear partial differential integral equation that describes the vibrations of a Bernoulli-Euler beam with initial curvatures. (2) The approximate analytical solutions of the discretized ordinary differential equations were obtained through the multiple scales method for the primary resonance. (3) We compared the analytical solutions with those of the finite element method. Based on the results obtained by the two methods, we found that the Galerkin method can accurately estimate the dynamic behaviors of beams without initial curvatures. On the contrary, the Galerkin method underestimates the softening effect of the quadratic nonlinear term that is induced by the initial curvature. This may cause erroneous results when the Galerkin method is used to study the dynamic behaviors of beams with the initial curvatures.
Research on Street Color Environment Perception Based on CEP-KASS Framework
Authors: Hu Kuntao , Xu Ziqi , Wang Xiufang , Wang Yingyu , Li Haoran , Zhang Yibing
Published: October 2023

The color of urban streets plays a crucial role in shaping a city’s image, enhancing street appeal, and optimizing the experience of citizens. Nevertheless, the relationship between street color environment and residents’ perceptions has rarely been deeply discussed, and most of the existing studies adopt qualitative methods. To accurately and effectively assess the connection between street color environment and residents’ emotional perceptions, this paper introduces a quantitative research framework based on multi-source data called “Color Emotion Perception with K-Means, Adversarial Strategy, SegNet, and SVM (CEP-KASS)”. By combining K-Means unsupervised machine learning and SegNet computer vision techniques, it captures and analyzes visual elements and color data from Baidu Street View Images (BSVI). It then employs a human–machine adversarial scoring model to quantify residents’ perceptions of BSVI and uses the support vector machine regression model to predict the final perception scores. Based on these data, a Pearson correlation analysis and visual analysis were conducted on the elements and color in the urban environment. Subsequently, the streets were classified based on perception frequency and perception scores by integrating multi-source data, and areas within the third ring of Xuzhou City were selected for validating the research framework. The results demonstrate that utilizing street-view images and the CEP-KASS framework can quantitatively analyze urban color perception and establish a connection with residents’ emotions. In terms of color perception, red, orange, and blue all have a strong positive correlation with the interesting score, whereas black is positively correlated with a sense of safety. Regarding color attributes, low-saturation bright colors result in higher fun perception scores in urban spaces; too low saturation and brightness can affect their attractiveness to residents; brightness has an inverse relationship with the perception of safety, and adjusting brightness inversely can improve the perceived safety experience in certain urban external spaces. The street classification criteria based on perception frequency and perception scores proposed herein can provide references for planners to prioritize color transformation decisions, with a priority on emulating HSHF streets and transforming LSHF streets. When formulating color planning, suggestions for color adjustment can be given based on the correlation study of color with visual elements and perception scores, optimizing urban residents’ spatial perception and their emotional experiences. These findings provide robust theoretical support for further enhancing the visual quality of streets and refining urban color planning.
Combination Therapy of Immune Checkpoint Inhibitors with Locoregional Therapy for Hepatocellular Carcinoma
Authors: Tamai Yasuyuki , Fujiwara Naoto , Tanaka Takamitsu , Mizuno Shugo , Nakagawa Hayato
Published: October 2023

Hepatocellular carcinoma (HCC) is estimated to be the fourth leading cause of cancer-related deaths globally, and its overall prognosis is dismal because most cases are diagnosed at a late stage and are unamenable to curative treatment. The emergence of immune checkpoint inhibitors (ICIs) has dramatically improved the therapeutic efficacy for advanced hepatocellular carcinoma; however, their response rates remain unsatisfactory, partly because >50% of HCC exhibit an ICI-nonresponsive tumor microenvironment characterized by a paucity of cytotoxic T cells (immune-cold), as well as difficulty in their infiltration into tumor sites (immune excluded). To overcome this limitation, combination therapies with locoregional therapies, including ablation, transarterial embolization, and radiotherapy, which are usually used for early stage HCCs, have been actively explored to enhance ICI efficacy by promoting the release of tumor-associated antigens and cytokines, and eventually accelerating the so-called cancer–immunity cycle. Various combination therapies have been investigated in early- to late-phase clinical trials, and some have shown promising results. This comprehensive article provides an overview of the immune landscape for HCC to understand ICI efficacy and its limitations and, subsequently, reviews the status of combinatorial therapies of ICIs with locoregional therapy for HCC.
Combination Therapy of Bland Transarterial Embolization and Microwave Ablation for Hepatocellular Carcinoma within the Milan Criteria Leads to Significantly Higher Overall Survival
Authors: Adwan Hamzah , Adwan Moath , Vogl Thomas J.
Published: October 2023

A comparison of the combination therapy consisting of microwave ablation (MWA) after bland lipiodol-based transarterial embolization (TAE) with MWA alone in the treatment of hepatocellular carcinoma (HCC) within the Milan criteria. Forty-nine patients in the TAE-MWA group (12 women and 37 men; mean age: 63.3 ± 9.6 years) with 55 tumors and 63 patients in the MWA group (18 women and 45 men; mean age: 65.9 ± 10.5 years) with 67 tumors were retrospectively enrolled in this study. For the investigation of treatment protocols based upon both safety and efficacy, patients’ cases were analyzed with regard to complications, local tumor progression (LTP), intrahepatic distant recurrence (IDR), overall survival (OS), and progression-free survival (PFS). There were no cases of major complications in either group. The LTP rate was 5.5% in the MWA-TAE group and 7.5% in the MWA group (p = 0.73). The rate of IDR was 42.9% in the MWA-TAE group and 52.4% in the MWA group (p = 0.42). The 12-, 24-, and 36-month OS rates starting at the date of tumor diagnosis were 97.7%, 85.1%, and 78.8% in the TAE-MWA group, and 91.9%, 71.4%, and 59.8% in the MWA group, respectively (p = 0.004). The 6-, 12-, and 24-month PFS rates were 76.5%, 55%, and 44.6% in the TAE-MWA group, and 74.6%, 49.2%, and 29.6% in the MWA group, respectively (p = 0.18). The combination therapy of TAE-MWA was significantly superior to MWA monotherapy according to OS in treating HCC within the Milan criteria.
New Generations of Tyrosine Kinase Inhibitors in Treating NSCLC with Oncogene Addiction: Strengths and Limitations
Authors: Attili Ilaria , Corvaja Carla , Spitaleri Gianluca , Del Signore Ester , Trillo Aliaga Pamela , Passaro Antonio , de Marinis Filippo
Published: October 2023

Tyrosine kinase inhibitors (TKIs) revolutionized the treatment of patients with advanced or metastatic non-small cell lung cancer (NSCLC) harboring most driver gene alterations. Starting from the first generation, research rapidly moved to the development of newer, more selective generations of TKIs, obtaining improved results in terms of disease control and survival. However, the use of novel generations of TKIs is not without limitations. We reviewed the main results obtained, as well as the ongoing clinical trials with TKIs in oncogene-addicted NSCLC, together with the biology underlying their potential strengths and limitations. Across driver gene alterations, novel generations of TKIs allowed delayed resistance, prolonged survival, and improved brain penetration compared to previous generations, although with different toxicity profiles, that generally moved their use from further lines to the front-line treatment. However, the anticipated positioning of novel generation TKIs leads to abolishing the possibility of TKI treatment sequencing and any role of previous generations. In addition, under the selective pressure of such more potent drugs, resistant clones emerge harboring more complex and hard-to-target resistance mechanisms. Deeper knowledge of tumor biology and drug properties will help identify new strategies, including combinatorial treatments, to continue improving results in patients with oncogene-addicted NSCLC.
A Novel ASCT2 Inhibitor, C118P, Blocks Glutamine Transport and Exhibits Antitumour Efficacy in Breast Cancer
Authors: Lyu Xiao-Dan , Liu Yang , Wang Jia , Wei Yuan-Cheng , Han Yi , Li Xue , Zhang Qian , Liu Zheng-Rui , Li Zheng-Zheng , Jiang Jing-Wei , Hu Hao-Lin , Yuan Sheng-Tao , Sun Li
Published: October 2023

Background: The microtubule protein inhibitor C118P shows excellent anti-breast cancer effects. However, the potential targets and mechanisms of C118P in breast cancer remain unknown. Methods: Real-time cellular analysis (RTCA) was used to detect cell viability. Apoptosis and the cell cycle were detected by flow cytometry. Computer docking simulations, surface plasmon resonance (SPR) technology, and microscale thermophoresis (MST) were conducted to study the interaction between C118P and alanine-serine-cysteine transporter 2 (ASCT2). Seahorse XF technology was used to measure the basal oxygen consumption rate (OCR). The effect of C118P in the adipose microenvironment was explored using a co-culture model of adipocytes and breast cancer cells and mouse cytokine chip. Results: C118P inhibited proliferation, potentiated apoptosis, and induced G2/M cell cycle arrest in breast cancer cells. Notably, ASCT2 was validated as a C118P target through reverse docking, SPR, and MST. C118P suppressed glutamine metabolism and mediated autophagy via ASCT2. Similar results were obtained in the adipocyte–breast cancer microenvironment. Adipose-derived interleukin-6 (IL-6) promoted the proliferation of breast cancer cells by enhancing glutamine metabolism via ASCT2. C118P inhibited the upregulation of ASCT2 by inhibiting the effect of IL-6 in co-cultures. Conclusion: C118P exerts an antitumour effect against breast cancer via the glutamine transporter ASCT2.
Ultra-Hypofractionated Re-Irradiation with Anti-PD-1 Immunotherapy for Locoregionally Recurrent (after Radical Chemo-Radiotherapy) Non-Small Cell Lung Cancer
Authors: Filippatos Konstantinos , Koukourakis Ioannis M. , Anevlavis Stavros , Giaktzidis Axiotis , Koukourakis Michael I.
Published: October 2023

Large fractions of radiotherapy of 8 Gy (ultra-hypofractionated RT, ultra-hypoRT) promote anti-tumor immune responses that have been clinically substantiated in combination trials with immune checkpoint inhibitors (ICIs). In the current study, we postulated that ultra-hypoRT in combination with ICIs may enhance tumor clearance in NSCLC patients with locoregional relapse after radical chemo-RT. Between 2019 and 2021, eleven patients received re-irradiation with one or two fractions of 8 Gy concurrently with anti-PD1 immunotherapy (nivolumab or pembrolizumab). RT-related toxicities were negligible, while immune-related adverse events enforced immunotherapy interruption in 36% of patients. The overall response rate was 81.8%. Tumor reduction between 80 and 100% was noted in 63.5% of patients. Within a median follow-up of 22 months, the locoregional relapse-free rate was 54.5%, while the projected 2-year disease-specific overall survival was 62%. The results were independent of PD-L1 status. The current report provides encouraging evidence that a relatively low biological dose of RT delivered with 8 Gy fractions is feasible and can be safely combined with anti-PD-1 immunotherapy. Despite the low number of patients, the significant tumor regression achieved and the long-lasting locoregional control and overall progression-free intervals provide a basis to pursue immuno-RT trials with U-hypoRT schemes in this group of NSCLC patients of poor prognosis.
Signal Amplification for Detection of Nilutamide in Three-Dimensional Electrochemical Sensor Using Copper Metal–Organic Framework Decorated Carbon Nanofibers
Authors: Elanthamilan Elaiyappillai , Wang Sea-Fue
Published: October 2023

The extensive use of antibiotics has rapidly spread antibiotic resistance, which poses significant health risks to humans. Unfortunately, despite this pressing issue, there is still a lack of a reliable on-site detection method for the residues of antibiotics, such as nilutamide (Nlu). Consequently, there is an urgent need to develop and perfect such a detection method to effectively monitor and control antibiotic residues. In this study, the hydrothermal development of copper-metal-organic framework (Cu-MOF) polyhedrons on the functionalized carbon nanofiber (f-CNF) matrix allowed for the detection of Nlu in biological liquids via a sensitive amperometry technique. Further electrochemical detection of Nlu took place with the cyclic voltammetry (CV) technique Cu-MOF/f-CNF. Analytical and spectroscopic approaches were used to confirm the successful synthesis of Cu-MOF/f-CNF. The prepared material was decorated on the surface of GCE and performed as an electrochemical Nlu sensor, with a broad linear range of 0.01 to 141.4 μM and 2 nM as a lower limit of detection. In addition, the composites had a large surface area and many dedicated sites, which improved electrocatalysis. In practical applications, Cu-MOF/f-CNF/GCE provides a novel strategy for improving electrochemical activity by measuring Nlu concentrations in biological samples.
Effect of Probiotic Dose Escalation on Gut Microbiota and Clinical Outcomes in Preterm Infants—A Systematic Review
Authors: Rath Chandra , Athalye-Jape Gayatri , Rao Shripada , Patole Sanjay
Published: October 2023

Probiotics are known to decrease incidences of necrotising enterocolitis, feeding intolerance, late-onset sepsis, and mortality in preterm infants. Administering an adequate dose is important for optimizing the benefits and safety of probiotics. We conducted a systematic review to assess the effect of probiotic dose escalation on clinical outcomes and gut microbiota in preterm neonates. We searched PubMed, EMBASE, EMCARE, Medline, Cochrane Library, Google Scholar, and MedNar databases in July 2023. Three studies were included. In one of the randomized studies (n = 149, gestation 27 to 33 weeks), no significant differences in faecal Lactobacillus and Bifidobacterium counts and clinical outcomes were seen between the high- and low-dose groups. There was a trend towards increased Lactobacillus and Bifidobacterium counts in the high-dose group. In the other randomized study (n = 120, birth weight 500 to 2000 gm), smaller infants (500 to 1000 gm) required higher doses to display Lactobacillus in their faeces. The cohort study (n = 12, gestation < 33 weeks) showed a trend towards an increase in faecal abundance of bifidobacteria and bacterial diversity in the B. infantis group with increasing dose/time. Limited evidence suggests a higher dose might improve gut colonization in preterm infants. Further studies are urgently needed to address this gap in the knowledge considering the increasing use of probiotics for preterm infants.
Polychromy in the Iberian Sculptures of Cerrillo Blanco: Analytical Study, Historical Context and State of Conservation
Authors: Romero-Noguera Julio , Ruiz-Ruiz María Belén , Doménech-Carbó María Teresa , Bolívar-Galiano Fernando
Published: October 2023

In the environs of the city of Ipolca, today’s town of Porcuna (Jaén), the Iberian civilisation left behind one of the most outstanding sculptural ensembles of Antiquity, made up of 27 groups of figures and hundreds of fragments dating from the 7th to the 2nd centuries BC. Despite its great relevance, there are very few scientific studies that serve as a basis for understanding the many questions that remain about how they were made, their significance, and their relationship to the culture that gave rise to them. This article studies the polychrome techniques used in the sculptures and puts them into context in Iberian art. The research has been carried out on original pieces from the Archaeological Museum of Jaén using stereoscopic optical microscopy (SOM), petrographic microscopy (PM), Fourier-transform infrared spectroscopy (FTIR), and scanning electron microscopy-energy dispersive X-ray analysis (SEM-EDX).
Study on the Rapid Degradation Performance of Salix/Wheat Straw Fiber Degradable Film
Authors: Liang Yuehui , Zhang Qian , Wang Bo , Liang Zhanpeng , Wang Xin
Published: October 2023

The preparation of biodegradable mulch film to replace non-degradable mulch film is of great significance for reducing the harm of non-degradable agricultural mulch film to the environment. However, there are few studies on the degradation performance and degradation mechanisms of degradable cellulose mulch. Therefore, the wet papermaking process was adopted in this work. Salix fiber and wheat straw fiber were used as raw materials. A Salix/wheat straw fiber degradable film was prepared by adding cationic polyacrylamide, alkyl ketene dimer, and paraffin emulsion. The degradation process of cellulose film was studied using a UV degradation test and an acid-base degradation test system. The results showed that after 40 days of UV degradation, the degradation rate of Salix/wheat straw fiber degradable film could reach 6.66%. The tensile strength could still maintain 2.878 KN/m. The results of the brightness change index (ΔL) and color overall change index (ΔE) showed that the surface of the Salix/wheat straw fiber degradable film had been successfully partially degraded. After 4 days of alkaline degradation, the degradation rate could reach 11.89%. After 4 days of acid degradation, the degradation rate could reach 14.64%. At the same time, the specific degradation process of Salix/wheat straw fiber degradable film was further studied by infrared spectroscopy and scanning electron microscopy. This work provides a new method for the study of agricultural degradable cellulose mulch, which is of great significance for the future development of agricultural mulch.
A Narrative Review on Non-Cirrohotic Portal Hypertension: Not All Portal Hypertensions Mean Cirrhosis
Authors: Fiordaliso Michele , Marincola Giuseppe , Pala Barbara , Muraro Raffaella , Mazzone Mariangela , Di Marcantonio Maria Carmela , Mincione Gabriella
Published: October 2023

Non-cirrhotic portal hypertension (NCPH), also known as idiopathic non-cirrhotic portal hypertension (INCPH) and porto-sinusoidal vascular disorder (PSVD), is a rare disease characterized by intrahepatic portal hypertension (IPH) in the absence of cirrhosis. The precise etiopathogenesis of IPH is an area of ongoing research. NCPH diagnosis is challenging, as there are no specific tests available to confirm the disease, and a high-quality liver biopsy, detailed clinical information, and an expert pathologist are necessary for diagnosis. Currently, the treatment of NCPH relies on the prevention of complications related to portal hypertension, following current guidelines of cirrhotic portal hypertension. No treatment has been studied that aimed to modify the natural history of the disease; however, transjugular intrahepatic porto-systemic shunt (TIPS) placement, shunt and liver transplantation are considerable symptomatic options. In this review, we discuss the heterogeneity of NCPH as well as its etiopathogenesis, clinical presentation and management issues. Starting from the assumption that portal hypertension does not always mean cirrhosis, cooperative studies are probably needed to clarify the issues of etiology and the possible genetic background of this rare disease. This knowledge might lead to better treatment and perhaps better prevention.
The Endoscopic Retrograde Cholangiopancreatography and Endoscopic Ultrasound Connection: Unity Is Strength, or the Endoscopic Ultrasonography Retrograde Cholangiopancreatography Concept
Authors: De Angelis Claudio Giovanni , Dall’Amico Eleonora , Staiano Maria Teresa , Gesualdo Marcantonio , Bruno Mauro , Gaia Silvia , Sacco Marco , Fimiano Federica , Mauriello Anna , Dibitetto Simone , Canalis Chiara , Stasio Rosa Claudia , Caneglias Alessandro , Mediati Federica , Rocca Rodolfo
Published: October 2023

Endoscopic ultrasound (EUS) and endoscopic retrograde cholangiopancreatography (ERCP) are both crucial for the endoscopic management of biliopancreatic diseases: the combination of their diagnostic and therapeutic potential is useful in many clinical scenarios, such as indeterminate biliary stenosis, biliary stones, chronic pancreatitis and biliary and pancreatic malignancies. This natural and evident convergence between EUS and ERCP, which by 2006 we were calling the “Endoscopic ultrasonography retrograde colangiopancreatography (EURCP) concept”, has become a hot topic in the last years, together with the implementation of the therapeutic possibilities of EUS (from EUS-guided necrosectomy to gastro-entero anastomoses) and with the return of ERCP to its original diagnostic purpose thanks to ancillary techniques (extraductal ultrasound (EDUS), intraductal ultrasound (IDUS), cholangiopancreatoscopy with biopsies and probe-based confocal laser endomicroscopy (pCLE)). In this literary review, we retraced the recent history of EUS and ERCP, reported examples of the clinical applicability of the EURCP concept and explored the option of performing the two procedures in only one endoscopic session, with its positive implications for the patient, the endoscopist and the health care system. In the last few years, we also evaluated the possibility of combining EUS and ERCP into a single endoscopic instrument in a single step, but certain obstacles surrounding this approach remain.
Metabolic Dysfunction-Associated Fatty Liver Disease in Taiwanese Patients with Inflammatory Bowel Disease: A Study in Patients with Clinical Remission
Authors: Hsiao Shun-Wen , Chen Ting-Chun , Su Pei-Yuan , Yang Chen-Ta , Huang Siou-Ping , Chen Yang-Yuan , Yen Hsu-Heng
Published: October 2023

The prevalence of inflammatory bowel disease (IBD) has increased worldwide. The prevalence of metabolic dysfunction associated fatty liver disease (MAFLD) has also risen. However, there is limited research on the connection between MAFLD and IBD in the Asian population. This study aims to analyze the prevalence and clinical significance of MAFLD in Taiwanese IBD patients with clinical remission. We retrospectively analyzed IBD patients who received transient elastography for liver fibrosis and controlled attenuation parameter evaluation for liver steatosis. This study enrolled 120 patients with IBD, including 45 Crohn’s disease (CD) and 75 ulcerative colitis (UC). MAFLD prevalence in IBD was 29.2%. Patients with MAFLD had a shorter disease duration (2.8 years vs. 5.3 years, p = 0.017), higher alanine aminotransferase levels (24 U/L vs. 17 U/L, p = 0.003), a lower estimated glomerular filtration rate (91.37 mL/min/1.73 m2 vs. 103.92 mL/min/1.73 m2, p = 0.004), and higher γ-glutamyl transferase (γ-GT) (24 mg/dL vs. 13 mg/dL, p < 0.001). The prevalence of significant fibrosis in IBD with MAFLD was 17.1%. Significant fibrosis was found in older age (58.5 years vs. 40 years, p = 0.004) and the high type 2 diabetes mellitus proportion (50.0% vs. 10.3%, p = 0.049). A trend of longer disease duration was found in significant fibrosis (4.9 years vs. 1.6 years, p = 0.051). The prevalence of MALFD in IBD was 29.2%. and 17.1% of them had significant fibrosis. In addition to the intestinal manifestation, the study findings remind clinicians that they should be aware of the possibility of hepatic complications for IBD patients.
A Hydraulic Online Monitoring System for Forestry Harvesters Based on LabVIEW
Authors: Qin Haoxian , Xu Jingwei , Wang Jianli , Huang Qingqing , Ma Yuewei , Liu Jinhao
Published: October 2023

The hydraulic system is a key component of intelligent forestry harvesters. In the testing of a forestry harvester, researchers need to analyze the operating efficiency and energy consumption of the forestry harvester based on the pressure and flow rate data in the hydraulic system of the forestry harvester and formulate energy-efficient control strategies. In order to enable researchers to monitor and extract the parameters of the hydraulic system of an intelligent forestry harvester in real time, this paper designs a hydraulic online monitoring system for forestry harvesters based on the LabVIEW 2019 software platform. This system realizes the following functions by reading the CAN (controller area network, a serial communication protocol for multi-host localized networks) bus of the harvester: (1) it collects and stores hydraulic system pressure, flow, and other data and displays the value curve in the system interface in real time, and (2) it monitors the control signals received by the hydraulic system and displays the control signal status and value received by the system interface in real time. This paper used this system to carry out on-site testing of an actual machine, and compared and analyzed the online monitoring data of the hydraulic system and the theoretical pressure data of the main hydraulic valve manifold. The average value of the relative error between the two was 1.65%, and the maximum value of the relative error was 2.75%. The results show that the designed system has good accuracy and stability, and can effectively realize online monitoring of the working status of the hydraulic system of forestry harvesters during their operation.
Effects of Gap Size on Natural Regeneration in Picea asperata Forests of Northern China
Authors: Yang Xin , Li Jiajing , Fan Niqiao , Wang Yiwen , Zhang Zhidong
Published: October 2023

Our study aimed to assess the impacts of varying forest gap sizes on the density, growth, and spatial patterns of seedlings and saplings in spruce (Picea asperata) forests in the Saihanba region, Hebei Province, China. Twenty-four forest gaps were surveyed and categorized into six classes based on the gap size. A one-way ANOVA was used to compare differences in the density, height, and ground diameter of seedlings and saplings among six gap classes. Ripley’s K function was used to explore the spatial patterns of regeneration establishment in each class. The findings of our study indicated that the forest gap size did not significantly influence the density of seedlings or the ground diameter growth of saplings, whereas it significantly influenced the height growth of saplings. In smaller gaps, natural regeneration occurred primarily in the gap edges. As the gap size increased, the natural generation began to shift from the edge areas to the gap centers. Large forest gaps had the highest percentages of random distribution patterns across all spatial scales. Aggregated distributions were observed at distances less than 1 m in all gap size classes, whereas uniform distributions tended to occur in the small gaps at distances of 2–4 m. Our findings indicated that larger forest gaps, ranging from 60 to 120 m2, were more conducive to spruce regeneration. The results can inform the development of targeted strategies for understory afforestation and the artificial promotion of natural regeneration in spruce forests.
Flame-Retardant and Smoke-Suppression Properties of Bamboo Scrimber Coated with Hexagonal Boron Nitride
Authors: Li Gaihuan , Yu Ying , Yuan Shaofei , Zhang Wenfu , Wang Xinzhou
Published: October 2023

In order to improve the flame-retardant properties of bamboo scrimber, chitosan (CS) and polyvinyl alcohol (PVA) were used as the film-forming substances, and hexagonal boron nitride (h-BN) was used as the flame-retardant substance to prepare h-BN flame-retardant coatings, which were coated on the surface of the bamboo scrimber. The effects of the h-BN flame-retardant coatings with different quality concentrations on the flame-retardant properties of the bamboo scrimber, as well as on the morphology of the residual carbon, were investigated using the analytical methods of FTIR, environmental scanning electron microscopy, thermogravimetric analysis, combustion test, and coating adhesion test. The results showed that the h-BN flame-retardant coating could improve the thermal stability of the bamboo scrimber and that the higher the mass concentration, the better the thermal stability of the h-BN. Compared to the control, the time to ignition (TTI) of the 5% h-BN flame-retardant-treated specimens increased by 56%; the peak heat release rate (Pk-HRR), total heat release (THR), and total smoke production (TSP) decreased by 9.92%, 7.54%, and 32.35%, respectively; however, due to the presence of PVA, the peak smoke production rate (Pk-SPR) increased by 17.78%. The 5% h-BN coating had very good adhesion, with an adhesion grade of zero. In conclusion, the h-BN coating could be well-adhered to the surface of the bamboo scrimber, and the 5% h-BN flame-retardant coating had a better flame retardancy compared to other treatments, meaning that it could provide a new strategy for improving the flame-retardant properties of bamboo scrimber for construction use.
Pine Wood Nematode’s Migration and Defense Mechanism of Highly Resistant and Susceptible Pinus massoniana
Authors: Li Wenhua , Zuo Yifan , Deng Lili , Xie Yini , Gao Kai , Zhou Zhichun , Liu Qinghua
Published: October 2023

Pine wilt disease has caused great economic loss and become an ecological threat since it was introduced into East Asia. In China, Pinus massoniana Lamb. is highly susceptible. The pathogenic of this disease is linked to the invasion of P. massoniana by the pine wood nematode (PWN, Bursaphelenchus xylophilus), leading to various physiological activities. However, the migration pathway of PWN and the defense mechanisms of P. massoniana tissue structure following invasion remain unclear. This knowledge is vital for understanding the pathogenesis of pine wood nematode disease. To address this issue, we analyzed the tissue structure damage, horizontal and vertical migration pathways, and histochemical reactions of P. massoniana after PWN inoculation. The results are as follows: susceptible P. massoniana exhibited more tissue structure damage compared to highly resistant P. massoniana. PWN reproduced and migrated by feeding on and damaging cells. In susceptible P. massoniana, PWN propagated and migrated throughout the entire plant. Highly resistant P. massoniana displayed limited horizontal and vertical migration of PWN, making it challenging for PWNs to move from cambium to xylem. After P. massoniana was damaged by PWNs, a protein cross-linking phenomenon appeared rapidly, with highly resistant P. massoniana exhibiting less protein cross-linking than the susceptible variety. Lignin synthesis is a crucial factor in the tissue defense of P. massoniana. Protein crosslinking provides time for lignin synthesis and is an vital component of tissue defense.
The Natural Growth of CaCO3 Crystals on Hemp Yarns: A Morphology Analysis and the Mechanical Effects on Composites
Authors: Drouhet Quentin , Barbière Romain , Touchard Fabienne , Chocinski-Arnault Laurence , Mellier David
Published: October 2023

Plant fibres are promising candidates to replace synthetic fibres in polymer matrix composites. However, there is still an important issue to overcome: the poor quality of adhesion at the fibre/matrix interface. Many surface treatments of plant fibres have been developed, most of them based on non-environmentally friendly processes. In this paper, a 100% natural treatment is proposed. Hemp yarns are immersed in tap water until the natural growth of limestone beads attached to their surface occurs. The morphology analysis reveals that these calcium carbonate crystals have a nanoneedle architecture, with hemp fibres acting as nucleators for these highly ordered coral-like structures. Tensile tests on ±45° woven hemp/epoxy composites show that the presence of CaCO3 beads improves the adhesion quality of the fibre/matrix interface and, therefore, increases Young’s modulus value.