bluuebunny/crossref_metadata_2025 huggingface dataset:
Vegetation Dynamics and Their Response to Climate Changes and Human Activities: A Case Study in the Hanjiang River Basin, China
Authors: Zhang Zizheng , Liang Siyuan , Xiong Yuqing
Published: March 2023

The Hanjiang River Basin (HJRB) is an important water conservation and ecological barrier area for the South–North Water Transfer Central Project. The quantitative analysis of regional differences in vegetation changes and their main drivers is important for the monitoring of the ecological environment of the basin and formulation of ecological protection measures. Based on MODIS13Q1 data from 2000 to 2020, spatiotemporal variation characteristics of vegetation in the HJRB were analyzed using Theil–Sen + Mann–Kendall, the Hurst index, and correlation analysis. Then, we detected the drivers using an optimal parameter geographic detector. The results showed that from 2000 to 2020, the average NDVI value increased from 0.651 to 0.737, with a spatial distribution pattern of “high in the northwest and low in the southeast”, and 88.68% of the study area showed an increase in vegetation cover, while 5.80% showed a significant degradation. The positive persistence of future vegetation changes is stronger than the negative. It may show a slowdown or degradation trend, among which the vegetation restoration along the Han River and urbanized areas need to be strengthened. The factor detector indicated that the main factors influencing vegetation change were topography and climate, for which the most influential variables, respectively, were elevation (0.1979), landform (0.1720), slope (0.1647), and soil type (0.1094), with weaker influence from human activity factors. The interaction test results showed that the interaction of various geographic factors enhanced the explanatory power of vegetation changes and showed mainly nonlinear and two-factor enhancements. The dominant factor varies between sub-basins; for example, the interaction between wind speed and land use conversion was the dominant factor in the middle reaches of the HJRB; the dominant factor in the lower reaches of the HJRB was expressed as the interaction between land use conversion and temperature. Finally, the effects of the range or category of different drivers on vegetation growth were systematically analyzed. The results of the study contribute to the understanding of the dynamic changes of vegetation based on a comprehensive consideration of the interaction of topography, climate, and human activities, taking into account the totality and variability of the geographical environment, and provide a reference for the ecological restoration and rational use of vegetation resources in the HJRB.
Upgrading Major Waste Streams Derived from the Biodiesel Industry and Olive Mills via Microbial Bioprocessing with Non-Conventional Yarrowia lipolytica Strains
Authors: Sarris Dimitris , Tsouko Erminta , Kothri Maria , Anagnostou Maria , Karageorgiou Eleni , Papanikolaou Seraphim
Published: March 2023

This study reports the development of a bioprocess involving the valorization of biodiesel-derived glycerol as the main carbon source for cell proliferation of Yarrowia lipolytica strains and production of metabolic compounds, i.e., citric acid (Cit), polyols, and other bio-metabolites, the substitution of process tap water with olive mill wastewater (OMW) in batch fermentations, and partial detoxification of OMW (up to 31.1% decolorization). Increasing initial phenolics (Phen) of OMW-glycerol blends led to substantial Cit secretion. Maximum Cit values, varying between 64.1–65.1 g/L, combined with high yield (YCit/S = 0.682–0.690 g Cit/g carbon sources) and productivity (0.335–0.344 g/L/h) were achieved in the presence of Phen = 3 g/L. The notable accumulation of endopolysaccharides (EPs) on the produced biomass was determined when Y. lipolytica LMBF Y-46 (51.9%) and ACA-YC 5033 (61.5%) were cultivated on glycerol-based media. Blending with various amounts of OMW negatively affected EPs and polyols biosynthesis. The ratio of mannitol:arabitol:erythritol was significantly affected (p < 0.05) by the fermentation media. Erythritol was the major polyol in the absence of OMW (53.5–62.32%), while blends of OMW-glycerol (with Phen = 1–3 g/L) promoted mannitol production (54.5–76.6%). Nitrogen-limited conditions did not favor the production of cellular lipids (up to 16.6%). This study addressed sustainable management and resource efficiency enabling the bioconversion of high-organic-load and toxic waste streams into valuable products within a circular bioeconomy approach.
Effect of Dioscorea Opposite Waste Supplementation on Antioxidant Capacity, Immune Response and Rumen Microbiome in Weaned Lambs
Authors: Yang Ruochen , Guo Yunxia , Zhang Shuo , Hao Qinghong , Duan Chunhui , Wang Yong , Ji Shoukun , Yan Hui , Zhang Yingjie , Liu Yueqin
Published: March 2023

Dioscorea opposite waste (DOW) has been shown to improve the gastrointestinal microbiome, antioxidation capacity, and immune activity, indicating it is a potential feed resource to improve the physiological health and rumen function of weaned lambs. In the present study, the responses of rumen microbiome to DOW supplementation in diet were profiled using metagenome sequencing. In addition, the potential of DOW to regulate plasma parameters in weaned lambs and its possible mechanisms were investigated. Sixty healthy male small tail Han lambs (22.68 ± 2.56 kg) were selected and equally assigned to four dietary treatments: (1) DOW-free diet (CON), (2) addition of 10% DOW diet (DOW1), (3) addition of 15% DOW diet (DOW2), and (4) addition of 20% DOW diet (DOW3). Experimental lambs were fed a corresponding diet for 62 days. Rumen microbiome and plasma parameters were determined at the end of the experiment. The results showed that dietary supplementation with DOW linearly increased the concentration of aspartate aminotransferase, alkaline phosphatase, Immunoglobulin A, Immunoglobulin M, Immunoglobulin G, Glutathione peroxidase, Superoxide dismutase, and total antioxidant capacity in the plasma of weaned lambs, but an opposite trend was observed in Interleukin-1β, Interleukin-6, tumor necrosis factor-α, and Malondialdehyde between the DOW-supplemented group and the CON group. Sequencing of rumen metagenome revealed that dietary supplementation with 20% DOW significantly affected the microbial composition and function and increased the richness and diversity of rumen microbiota and relative abundance of phylum Verrucomicrobia, Planctomycetes, Fibrobacteres, Chloroflexi, Actinobacteria, and Acidobacteria and species Ruminococcaceae_bacterium, Clostridiales_bacterium_NK3B98, Clostridiales_bacterium, and Clostridia_bacterium. It was concluded that supplementing the weaned lamb’s ration with DOW increased the immune response and antioxidant capacity in a dose-dependent manner. Meanwhile, dietary supplementation with 20% DOW modulated the composition of rumen microbiome function by increasing Ruminococcaceae_bacterium and Clostridiales_bacterium with improving the polysaccharide hydrolase activity in the rumen.
Simulation of Acoustic Properties of Plaster Matrix Composite MATERIAL Reinforced with Corn Stem Fibers
Authors: Puyana-Romero Virginia , Jaramillo Cevallos Wilson Andrés , Ciaburro Giuseppe
Published: March 2023

Environmental sustainability and environmental protection are key to shaping the built environment. The use of environmentally sustainable materials in architecture is essential to transform urban centers into modern, sustainable cities, reducing the pollution of air and natural ecosystems, lowering gas emissions, and improving the energy efficiency of structures. In this study, corn processing waste was used as a reinforcing material to create a plaster matrix composite material for use as a sound absorption material. Specimens of two thicknesses were created, and the sound absorption coefficient (SAC) was measured by applying the normal incidence technique. Subsequently, a simulation model for predicting SAC using Artificial Neural Network (ANN) algorithms was utilized to compare the absorption performance of the specimens. The fibers extracted from the corn stem significantly improved the sound absorption performance of the gypsum matrix specimens. This is due to the increase in the porosity of the material caused by the adhesion between the fiber and the plaster which creates air pockets due to the roughness of the fiber. The simulation model appears to be effective in predicting the absorption properties of the material, as indicated by the results.
Unrecorded Tundra Fires of the Arctic Slope, Alaska USA
Authors: Miller Eric A. , Jones Benjamin M. , Baughman Carson A. , Jandt Randi R. , Jenkins Jennifer L. , Yokel David A.
Published: March 2023

Few fires are known to have burned the tundra of the Arctic Slope north of the Brooks Range in Alaska, USA. A total of 90 fires between 1969 and 2022 are known. Because fire has been rare, old burns can be detected by the traces of thermokarst and distinct vegetation they leave in otherwise uniform tundra, which are visible in aerial photograph archives. Several prehistoric tundra burns have been found in this way. Detection of tundra fires in this sparsely populated and remote area has been historically inconsistent and opportunistic, relying on reports by aircraft pilots. Fire reports have been logged into an administrative database which, out of necessity, has been used to scientifically evaluate changes in the fire regime. To improve the consistency of the record, we completed a systematic search of Landsat Collection 2 for the Brooks Range Foothills ecoregion over the period 1972–2022. We found 57 unrecorded tundra burns, about 41% of the total, which now numbers 138. Only 15% and 33% of all fires appear in MODIS and VIIRS satellite-borne thermal anomaly products, respectively. The fire frequency in the first 37 years of the record is 0.89 y−1 for natural ignitions that spread ≥10 ha. Frequency in the last 13 years is 2.5 y−1, indicating a nearly three-fold increase in fire frequency.
Computational Fluid Dynamics Modelling of Two-Phase Bubble Columns: A Comprehensive Review
Authors: Besagni Giorgio , Varallo Nicolò , Mereu Riccardo
Published: March 2023

Bubble columns are used in many different industrial applications, and their design and characterisation have always been very complex. In recent years, the use of Computational Fluid Dynamics (CFD) has become very popular in the field of multiphase flows, with the final goal of developing a predictive tool that can track the complex dynamic phenomena occurring in these types of reactors. For this reason, we present a detailed literature review on the numerical simulation of two-phase bubble columns. First, after a brief introduction to bubble column technology and flow regimes, we discuss the state-of-the-art modelling approaches, presenting the models describing the momentum exchange between the phases (i.e., drag, lift, turbulent dispersion, wall lubrication, and virtual mass forces), Bubble-Induced Turbulence (BIT), and bubble coalescence and breakup, along with an overview of the Population Balance Model (PBM). Second, we present different numerical studies from the literature highlighting different model settings, performance levels, and limitations. In addition, we provide the errors between numerical predictions and experimental results concerning global (gas holdup) and local (void fraction and liquid velocity) flow properties. Finally, we outline the major issues to be solved in future studies.
Valorization of Wild Edible Plants as Food Ingredients and Their Economic Value
Authors: Clemente-Villalba Jesús , Burló Francisco , Hernández Francisca , Carbonell-Barrachina Ángel A.
Published: March 2023

(1) Background: Wild Edible Plants (WEPs) are plants that grow without human help, by simply using the available resources. These types of plants are undervalued, because there is a lack of knowledge about their bioactive composition and nutritional/functional potential. (2) Scope and Approach: The main aim of this review is to fully identify the potential uses and importance of WEPs in certain regions based on (i) their sustainability, because they grow with their own resources, (ii) their content of bioactive compounds and consequently nutritional and functional value, (iii) their socio-economic relevance, and (iv) their ability to be useful in the agri-food industry in the short term. (3) Results: This review found evidence that a consumption of between 100 and 200 g of some of these WEPs can cover up to 50% of the recommended daily intake of proteins and fiber, being also a natural source of macro- and micro-minerals. Regarding their bioactive composition, most of these plants contain phenolic compounds and flavonoids, which determine their antioxidant capacity. (4) Conclusions: These reported results clearly demonstrate the high potential of the WEPs from a nutritional, economic and social point of view; although further studies are needed to gather deeper scientific information about their potential role in the socio-economic sustainability of specific groups of farmers worldwide.
Exploring the Impact of α-Amylase Enzyme Activity and pH on Flavor Perception of Alcoholic Drinks
Authors: Santos Maria João , Correia Elisete , Vilela Alice
Published: March 2023

The introduction of a drink in the mouth and the action of saliva and enzymes cause the perception of basic tastes and some aromas perceived in a retro-nasal way. Thus, this study aimed to evaluate the influence of the type of alcoholic beverage (beer, wine, and brandy) on lingual lipase and α-amylase activity and in-mouth pH. It was possible to see that the pH values (drink and saliva) differed significantly from the pH values of the initial drinks. Moreover, the α-amylase activity was significantly higher when the panel members tasted a colorless brandy, namely Grappa. Red wine and wood-aged brandy also induced greater α-amylase activity than white wine and blonde beer. Additionally, tawny port wine induced greater α-amylase activity than red wine. The flavor characteristics of red wines due to skin maceration and the contact of the brandy with the wood can cause a synergistic effect between beverages considered “tastier” and the activity of human α-amylase. We can conclude that saliva-beverage chemical interactions may depend on the saliva composition but also on the chemical composition of the beverage, namely its constitution in acids, alcohol concentration, and tannin content. This work is an important contribution to the e-flavor project, the development of a sensor system capable of mimicking the human perception of flavor. Furthermore, a better understanding of saliva–drink interactions allow us to comprehend which and how salivary parameters can contribute to taste and flavor perception.
Fluorescent and Colorimetric Dual-Mode Strategy Based on Rhodamine 6G Hydrazide for Qualitative and Quantitative Detection of Hg2+ in Seafoods
Authors: Zhang Ziwen , Han Ran , Chen Sixuan , Zheng Feilin , Ma Xinmiao , Pan Mingfei , Wang Shuo
Published: March 2023

In this study, a rapid fluorescent and colorimetric dual-mode detection strategy for Hg2+ in seafoods was developed based on the cyclic binding of the organic fluorescent dye rhodamine 6G hydrazide (R6GH) to Hg2+. The luminescence properties of the fluorescent R6GH probe in different systems were investigated in detail. Based on the UV and fluorescence spectra, it was determined that the R6GH has good fluorescence intensity in acetonitrile and good selective recognition of Hg2+. Under optimal conditions, the R6GH fluorescent probe showed a good linear response to Hg2+ (R2 = 0.9888) in the range of 0–5 μM with a low detection limit of 2.5 × 10−2 μM (S/N = 3). A paper-based sensing strategy based on fluorescence and colorimetric analysis was developed for the visualization and semiquantitative analysis of Hg2+ in seafoods. The LAB values of the paper-based sensor impregnated with the R6GH probe solution showed good linearity (R2 = 0.9875) with Hg2+ concentration in the range of 0–50 μM, which means that the sensing paper can be combined with smart devices to provide reliable and efficient Hg2+ detection.
Correlation between Water Characteristics and Gel Strength in the Gel Formation of Golden Pompano Surimi Induced by Dense Phase Carbon Dioxide
Authors: Duan Weiwen , Qiu Hui , Htwe Kyi Kyi , Wang Zefu , Liu Yang , Wei Shuai , Xia Qiuyu , Sun Qinxiu , Han Zongyuan , Liu Shucheng
Published: March 2023

The relationship between the gel quality of golden pompano surimi treated with dense phase carbon dioxide (DPCD) and changes in water characteristics was evaluated. Low-field nuclear magnetic resonance (LF-NMR) and nuclear magnetic resonance imaging were used to monitor changes in the water status of surimi gel under different treatment conditions. Whiteness, water-holding capacity and gel strength were used as the quality indicators of the surimi gel. The results showed that DPCD treatment could significantly increase the whiteness of surimi and the strength of the gel, while the water-holding capacity decreased significantly. LF-NMR analysis showed that, as the DPCD treatment intensity increased, the relaxation component T22 shifted to the right, T23 shifted to the left, the proportion of A22 decreased significantly (p < 0.05) and the proportion of A23 increased significantly (p < 0.05). A correlation analysis of water characteristics and gel strength showed that the water-holding capacity of surimi induced by DPCD was strongly positively correlated with gel strength, while A22 and T23 were strongly negatively correlated with gel strength. This study provides helpful insights into the quality control of DPCD in surimi processing and also provides an approach for the quality evaluation and detection of surimi products.
Impact of Longkong Pericarp Extract on the Physicochemical Properties of Alginate-Based Edible Nanoparticle Coatings and Quality Maintenance of Shrimp (Penaeus monodon) during Refrigerated Storage
Authors: Charoenphun Narin , Rajasekaran Bharathipriya , Palanisamy Suguna , Venkatachalam Karthikeyan
Published: March 2023

The objective of this study was to evaluate the impact of varying concentrations of longkong pericarp extract (LPE) on the physicochemical properties of alginate-based edible nanoparticle coatings (NP-ALG) on shrimp. For developing the nanoparticles, the alginate coating emulsion with different LPE concentrations (0.5, 1.0, and 1.5%) was ultrasonicated at 210 W with a frequency of 20 kHz for 10 min and a pulse duration of 1s on and 4 off. After that, the coating emulsion was separated into four treatments (T): T1: Coating solution containing basic ALG composition and without the addition of LPE or ultrasonication treatment; T2: ALG coating solution converted into nano-sized particles with ultrasonication and containing 0.5% LPE; T3: ALG coating solution converted into nano-sized particles with ultrasonication and containing 1.0% LPE; T4: ALG coating solution converted into nano-sized particles with ultrasonication and containing 1.5% LPE. A control (C) was also used, where distilled water was used instead of ALG coating. Before coating the shrimp, all the coating materials were tested for pH, viscosity, turbidity, whiteness index, particle size, and polydispersity index. The control samples had the highest pH and whiteness index and was followed by the lowest viscosity and turbidity (p < 0.05). Among the T1–T4 coating materials, T4 coating had higher turbidity, particle size, polydispersity index, but lower pH, viscosity, and whiteness index (p < 0.05). To study the quality and shelf-life of the shrimp, all coated shrimp samples were refrigerated at 4 °C for a period of 14 days. At 2-day intervals, physiochemical and microbial analyses were performed. The coated shrimp also had a lower increase in pH and weight loss over the storage period (p < 0.05). Coatings containing 1.5% LPE significantly reduced the polyphenol oxidase activity in the shrimp (p > 0.05). The addition of LPE to NP-ALG coatings demonstrated dose-dependent antioxidant activity against protein and lipid oxidation. The highest LPE concentration (1.5%) led to increased total and reactive sulfhydryl content, along with a significant decrease in carbonyl content, peroxide value, thiobarbituric acid reactive substances, p-anisidine, and totox values at the end of the storage period (p < 0.05). Additionally, NP-ALG-LPE coated shrimp samples exhibited an excellent antimicrobial property and significantly inhibited the growth of total viable count, lactic acid bacteria, Enterobacteriaceae, and psychotropic bacteria during storage. These results suggested that NP-ALG-LPE 1.5% coatings effectively maintained the quality as well as extended the shelf-life of shrimp during 14 days of refrigerated storage. Therefore, the use of nanoparticle-based LPE edible coating could be a new and effective way to maintain the quality of shrimp during prolonged storage.
Gas Chromatography with Flame-Ionization Detection-Based Analysis of Sugar Contents in Korean Agricultural Products for Patients with Galactosemia
Authors: Jeong Ha-Neul , Kwon Ryeong Ha , Kim Yuri , Yoo Sang-Ho , Yoo Seon Mi , Wee Chi-Do
Published: March 2023

Patients with galactosemia accumulate galactose in their bodies, requiring a lifelong galactose-restricted diet. Therefore, accurate information on the galactose content in commercial agro-food resources is essential. The HPLC method generally used for sugar analysis has low separation and detection sensitivity. Here, we sought to establish an accurate analytical method for determining the galactose content in commercial agro-food resources. To that aim, we employed gas chromatography with flame-ionization detection to detect trimethylsilyl-oxime (TMSO) sugar derivatives (concentration: ≤0.1 mg/100 g). The galactose content in 107 Korean agro-food resources reflecting intake patterns was then analyzed. The galactose content in steamed barley rice was 5.6 mg/100 g, which was higher than that in steamed non-glutinous and glutinous rice. Moist-type and dry-type sweet potatoes, blanched zucchini, and steamed Kabocha squash had high galactose content (36.0, 12.8, 23.1, and 61.6 mg/100 g, respectively). Therefore, these foods are detrimental to patients with galactosemia. Among fruits, avocado, blueberry, kiwi, golden kiwifruit, and sweet persimmon had galactose contents of ≥10 mg/100 g. Dried persimmon had 132.1 mg/100 g and should therefore be avoided. Mushrooms, meat, and aquatic products showed low galactose content (≤10 mg/100 g), making them safe. These findings will help patients to manage their dietary galactose intake.
Palmitic Acid Regulation of Stem Browning in Freshly Harvested Mini-Chinese Cabbage (Brassica pekinensis (Lour.) Rupr.)
Authors: Gao Hongdou , Zeng Shixian , Yue Xiaozhen , Yuan Shuzhi , Zuo Jinhua , Wang Qing
Published: March 2023

The effect of palmitic acid (PA) on stem browning was investigated in freshly harvested mini-Chinese cabbage (Brassica pekinensis). Results indicated that concentrations of PA ranging from 0.03 g L−1 to 0.05 g L−1 inhibited stem browning and decreased the rate of respiration, electrolyte leakage, and weight loss, as well as the level of malondialdehyde (MDA) in freshly harvested mini-Chinese cabbage stored at 25 °C for 5 d. The PA treatment enhanced the activity of antioxidant enzymes (ascorbate peroxidase (APX), catalase (CAT), peroxidase (POD), 4-coumarate:CoA ligase (4CL) and phenylalamine ammonia lyase (PAL)), and inhibited the activity of polyphenol oxidase (PPO). The PA treatment also increased the level of several phenolics (chlorogenic acid, gallic acid, catechin, p-coumaric acid, ferulic acid, p-hydroxybenzoic acid, and cinnamic acid) and flavonoids (quercetin, luteolin, kaempferol, and isorhamnetin). In summary, results indicate that treatment of mini-Chinese cabbage with PA represents an effective method for delaying stem browning and maintaining the physiological quality of freshly harvested mini-Chinese cabbage due to the ability of PA to enhance antioxidant enzyme activity and the level of phenolics and flavonoids during 5 d.
Caloric and Lipid Profiles during Pregnancy in a Socio-Culturally Diverse Society
Authors: Fernández-Gómez Elisabet , Mohatar-Barba Miriam , López-Olivares María , Luque-Vara Trinidad , Sánchez-Ojeda María Angustias , Martín-Salvador Adelina , Enrique-Mirón Carmen
Published: March 2023

This research analyzes the determining factors in diet quality among the Spanish pregnant population with the aim of promoting healthier eating habits and preventing the development of non-communicable diseases. It is a diagnostic, non-experimental, cross-sectional, and observational study, with correlational descriptive methodology, and 306 participants. The information was collected using the 24 h dietary recall. Various sociodemographic factors that influence diet quality were analyzed. It was found that pregnant women consume too much protein and fat, score high in SFA consumption, and do not achieve the CH recommendations, consuming twice as much sugar. Carbohydrate intake is inversely related to income (β = −0.144, p < 0.005). Likewise, protein intake is linked to marital status (β = −0.114, p < 0.005) and religion (β = 0.110, p < 0.005). Finally, lipid intake appears conditional upon age (β = 0.109, p < 0.005). As regards the lipid profile, a positive association is only observed with age and MFA consumption (β = 0.161, p < 0.01). On the other hand, simple sugars are positively related to education (β = 0.106, p < 0.005). The results of this research show that the diet quality of pregnant women does not meet the nutritional recommendations established for the Spanish population.
Development of a Turning Movement Estimator Using CV Data
Authors: Nazari Enjedani Somayeh , Khanal Mandar
Published: March 2023

Turning movement (TM) data of vehicular traffic at intersections are a basic input for signal timing design. Existing methods of collecting TM data are time- and cost-intensive. Using connected vehicle (CV) data is an alternative method. Trajectories of vehicles through an intersection can be constructed using CV data. However, because of the low number of CVs in the traffic stream, it is imprecise to consider TM data from CVs as representative of the whole traffic flow. To address this issue, a Kalman filter (KF) for estimating TM rates at intersections based on CV data under low market penetration levels using commercially available connected vehicle data was developed in this study. This method is independent of intersection geometry or the presence of shared lanes. The algorithm was evaluated using data from an intersection in Salt Lake City, Utah. The manually collected TM counts at this intersection were compared with the raw CV data as well as the results obtained from the developed methodology. The comparison shows that while TM counts based on raw CV data show severe violations in accuracy, making them unreliable, the method developed in this research gives results that have much lower accuracy violations.
Magnetism in High-Mass Stars
Authors: Keszthelyi Zsolt
Published: March 2023

Magnetism is a ubiquitous property of astrophysical plasmas, yet stellar magnetism still remains far from being completely understood. In this review, we describe recent observational and modelling efforts and progress to expand our knowledge of the magnetic properties of high-mass stars. Several mechanisms (magneto-convection, mass-loss quenching, internal angular momentum transport, and magnetic braking) have significant implications for stellar evolution, populations, and end-products. Consequently, it remains an urgent issue to address and resolve open questions related to magnetism in high-mass stars.
Single Hydrogel Particle Mechanics and Dynamics Studied by Combining Capillary Micromechanics with Osmotic Compression
Authors: Bakal Kalpit J. , Pollet Andreas M. A. O. , den Toonder Jaap M. J. , Wyss Hans M.
Published: March 2023

Hydrogels can exhibit a remarkably complex response to external stimuli and show rich mechanical behavior. Previous studies of the mechanics of hydrogel particles have generally focused on their static, rather than dynamic, response, as traditional methods for measuring single particle response at the microscopic scale cannot readily measure time-dependent mechanics. Here, we study both the static and the time-dependent response of a single batch of polyacrylamide (PAAm) particles by combining direct contact forces, applied by using Capillary Micromechanics, a method where particles are deformed in a tapered capillary, and osmotic forces are applied by a high molecular weight dextran solution. We found higher values of the static compressive and shear elastic moduli for particles exposed to dextran, as compared to water (KDex≈63 kPa vs. Kwater≈36 kPa, and GDex≈16 kPa vs. Gwater≈7 kPa), which we accounted for, theoretically, as being the result of the increased internal polymer concentration. For the dynamic response, we observed surprising behavior, not readily explained by poroelastic theories. The particles exposed to dextran solutions deformed more slowly under applied external forces than did those suspended in water (τDex≈90 s vs. τwater≈15 s). The theoretical expectation was the opposite. However, we could account for this behaviour by considering the diffusion of dextran molecules in the surrounding solution, which we found to dominate the compression dynamics of our hydrogel particles suspended in dextran solutions.
Recent Advances in Decellularized Matrix-Derived Materials for Bioink and 3D Bioprinting
Authors: Liu Huaying , Gong Yuxuan , Zhang Kaihui , Ke Shen , Wang Yue , Wang Jing , Wang Haibin
Published: March 2023

As an emerging 3D printing technology, 3D bioprinting has shown great potential in tissue engineering and regenerative medicine. Decellularized extracellular matrices (dECM) have recently made significant research strides and have been used to create unique tissue-specific bioink that can mimic biomimetic microenvironments. Combining dECMs with 3D bioprinting may provide a new strategy to prepare biomimetic hydrogels for bioinks and hold the potential to construct tissue analogs in vitro, similar to native tissues. Currently, the dECM has been proven to be one of the fastest growing bioactive printing materials and plays an essential role in cell-based 3D bioprinting. This review introduces the methods of preparing and identifying dECMs and the characteristic requirements of bioink for use in 3D bioprinting. The most recent advances in dECM-derived bioactive printing materials are then thoroughly reviewed by examining their application in the bioprinting of different tissues, such as bone, cartilage, muscle, the heart, the nervous system, and other tissues. Finally, the potential of bioactive printing materials generated from dECM is discussed.
Genome-Wide Association Study for Grain Protein, Thousand Kernel Weight, and Normalized Difference Vegetation Index in Bread Wheat (Triticum aestivum L.)
Authors: Krishnappa Gopalareddy , Khan Hanif , Krishna Hari , Devate Narayana Bhat , Kumar Satish , Mishra Chandra Nath , Parkash Om , Kumar Sachin , Kumar Monu , Mamrutha Harohalli Masthigowda , Singh Gyanendra Pratap , Singh Gyanendra
Published: March 2023

Genomic regions governing grain protein content (GPC), 1000 kernel weight (TKW), and normalized difference vegetation index (NDVI) were studied in a set of 280 bread wheat genotypes. The genome-wide association (GWAS) panel was genotyped using a 35K Axiom array and phenotyped in three environments. A total of 26 marker-trait associations (MTAs) were detected on 18 chromosomes covering the A, B, and D subgenomes of bread wheat. The GPC showed the maximum MTAs (16), followed by NDVI (6), and TKW (4). A maximum of 10 MTAs was located on the B subgenome, whereas, 8 MTAs each were mapped on the A and D subgenomes. In silico analysis suggest that the SNPs were located on important putative candidate genes such as NAC domain superfamily, zinc finger RING-H2-type, aspartic peptidase domain, folylpolyglutamate synthase, serine/threonine-protein kinase LRK10, pentatricopeptide repeat, protein kinase-like domain superfamily, cytochrome P450, and expansin. These candidate genes were found to have different roles including regulation of stress tolerance, nutrient remobilization, protein accumulation, nitrogen utilization, photosynthesis, grain filling, mitochondrial function, and kernel development. The effects of newly identified MTAs will be validated in different genetic backgrounds for further utilization in marker-aided breeding.
Structural and Functional Classification of G-Quadruplex Families within the Human Genome
Authors: Neupane Aryan , Chariker Julia H. , Rouchka Eric C.
Published: March 2023

G-quadruplexes (G4s) are short secondary DNA structures located throughout genomic DNA and transcribed RNA. Although G4 structures have been shown to form in vivo, no current search tools that examine these structures based on previously identified G-quadruplexes and filter them based on similar sequence, structure, and thermodynamic properties are known to exist. We present a framework for clustering G-quadruplex sequences into families using the CD-HIT, MeShClust, and DNACLUST methods along with a combination of Starcode and BLAST. Utilizing this framework to filter and annotate clusters, 95 families of G-quadruplex sequences were identified within the human genome. Profiles for each family were created using hidden Markov models to allow for the identification of additional family members and generate homology probability scores. The thermodynamic folding energy properties, functional annotation of genes associated with the sequences, scores from different prediction algorithms, and transcription factor binding motifs within a family were used to annotate and compare the diversity within and across clusters. The resulting set of G-quadruplex families can be used to further understand how different regions of the genome are regulated by factors targeting specific structures common to members of a specific cluster.
Genomic Diversity and Runs of Homozygosity in Bernese Mountain Dogs
Authors: Letko Anna , Hédan Benoît , Snell Anna , Harris Alexander C. , Jagannathan Vidhya , Andersson Göran , Holst Bodil S. , Ostrander Elaine A. , Quignon Pascale , André Catherine , Leeb Tosso
Published: March 2023

Bernese mountain dogs are a large dog breed formed in the early 1900s in Switzerland. While originally farm dogs that were used for pulling carts, guarding, and driving cattle, today they are considered multi-purpose companion and family dogs. The breed is predisposed to several complex diseases, such as histiocytic sarcoma, degenerative myelopathy, or hip dysplasia. Using whole-genome sequencing (WGS) data, we assessed the genomic architecture of 33 unrelated dogs from four countries: France, Sweden, Switzerland, and the United States. Analysis of runs of homozygosity (ROH) identified 12,643 ROH with an average length of 2.29 Mb and an average inbreeding coefficient of 0.395. Multidimensional scaling analysis of the genetic relatedness revealed limited clustering of European versus USA dogs, suggesting exchanges of breeding stock between continents. Furthermore, only two mtDNA haplotypes were detected in the 33 studied dogs, both of which are widespread throughout multiple dog breeds. WGS-based ROH analyses revealed several fixed or nearly fixed regions harboring discreet morphological trait-associated as well as disease-associated genetic variants. Several genes involved in the regulation of immune cells were found in the ROH shared by all dogs, which is notable in the context of the breed’s strong predisposition to hematopoietic cancers. High levels of inbreeding and relatedness, strongly exaggerated in the last 30 years, have likely led to the high prevalence of specific genetic disorders in this breed.
Factors Related to Nutritional Status of Single Older Residents in Semi-Mountainous Rural Regions of Japan: A Cross-Sectional Study
Authors: Nakai Ai , Morioka Ikuharu
Published: March 2023

Japan’s notably high aging rate presents the risk of malnutrition. This study aimed to clarify the nutritional status and factors related to the nutritional status of single older residents in a semi-mountainous rural region of Japan. Using a cross-sectional study design, surveys were administered to older adults in the semi-mountainous rural region in the area of Kochi Prefecture, Japan. Factors associated with a risk of malnutrition were identified using binomial logistic regression analysis. In addition, nutritional status was evaluated using the Mini Nutritional Assessment-Short Form (MNA-SF). Among 53 participants, the MNA-SF score was 12.1 ± 1.5 (mean ± standard deviation), and 71.7% had a normal nutritional status. We observed that participation in local residents’ association gatherings (odds ratio [OR]: 7.42, 95% confidence interval [CI]: 1.17–47.01) and risk of depression/anxiety (OR: 12.77, 95% CI: 1.99–81.94) were associated with an increased risk of malnutrition, whereas social interaction with friends (OR: 0.11, 95% CI: 0.02–0.76) were associated with a decreased risk. The nutritional status was normal overall. Community health workers should share information on the health of residents and promote social events to enable older residents living alone to continue leading healthy lifestyles.
Alpine Grassland Degradation and Its Restoration in the Qinghai–Tibet Plateau
Authors: Zhou Huakun , Yang Xiaoyuan , Zhou Chenyu , Shao Xinqing , Shi Zhengchen , Li Honglin , Su Hongye , Qin Ruimin , Chang Tao , Hu Xue , Yuan Fang , Li Shan , Zhang Zhonghua , Ma Li
Published: March 2023

The alpine grasslands of the Qinghai–Tibet Plateau are one of the most famous grazing ecosystems in the world, providing a variety of ecosystem functions and services. The rate of grassland degradation has been slowed by the implementation of national grassland restoration projects, but the degradation of grasslands on the Qinghai–Tibet Plateau has not yet been fundamentally reversed, and some grasslands are still degraded to varying degrees. The main causes of grassland degradation on the Qinghai–Tibet Plateau are both human and natural factors. Human factors include overgrazing, over-cultivation, indiscriminate digging and mining, mineral resource development, infrastructure construction and use, and tourism development. Natural factors include climate change, wildlife destruction, pests, etc. Based on the principles of restoration ecology, a number of effective practices and integrated management responses for restoring degraded grasslands have been developed on the Qinghai–Tibet Plateau. The degraded grassland restoration practices include fencing, fertilization, sown grassland establishment, rodent control, and grazing management. Based on these practices, the comprehensive restoration of degraded grasslands and the establishment and sustainable management of sown grasslands in the alpine grasslands of the Qinghai–Tibet Plateau should be further strengthened, and research on the mechanisms of grassland degradation and restoration should be further developed.
Super-Resolution Techniques in Photogrammetric 3D Reconstruction from Close-Range UAV Imagery
Authors: Panagiotopoulou Antigoni , Grammatikopoulos Lazaros , El Saer Andreas , Petsa Elli , Charou Eleni , Ragia Lemonia , Karras George
Published: March 2023

Current Multi-View Stereo (MVS) algorithms are tools for high-quality 3D model reconstruction, strongly depending on image spatial resolution. In this context, the combination of image Super-Resolution (SR) with image-based 3D reconstruction is turning into an interesting research topic in photogrammetry, around which however only a few works have been reported so far in the literature. Here, a thorough study is carried out on various state-of-the-art image SR techniques to evaluate the suitability of such an approach in terms of its inclusion in the 3D reconstruction process. Deep-learning techniques are tested here on a UAV image dataset, while the MVS task is then performed via the Agisoft Metashape photogrammetric tool. The data under experimentation are oblique cultural heritage imagery. According to results, point clouds from low-resolution images present quality inferior to those from upsampled high-resolution ones. The SR techniques HAT and DRLN outperform bicubic interpolation, yielding high precision/recall scores for the differences of reconstructed 3D point clouds from the reference surface. The current study indicates spatial image resolution increased by SR techniques may indeed be advantageous for state-of-the art photogrammetric 3D reconstruction.
5G Service and Pacemakers/Implantable Defibrillators: What Is the Actual Risk?
Authors: Vivarelli Cecilia , Censi Federica , Calcagnini Giovanni , De Ruvo Ermenegildo , Calò Leonardo , Mattei Eugenio
Published: March 2023

Rapidly growing worldwide, 5G service is expected to deeply change the way we communicate, connect and share data. It encompasses the whole spectrum of new technology, infrastructure and mobile connectivity, and will touch not only every sector in the industry, but also many aspects of our everyday life. Although the compliance with international regulations provides reasonable protection to public health and safety, there might be specific issues not fully covered by the current technical standards. Among the aspects that shall be carefully considered, there is the potential interference that can be induced on medical devices, and in particular on implantable medical devices that are critical for the patient’s life, such as pacemakers and implantable defibrillators. This study aims to assess the actual risk that 5G communication systems pose to pacemakers and implantable defibrillators. The setup proposed by the ISO 14117 standard was adapted to include 5G characteristic frequencies of 700 MHz and 3.6 GHz. A total number of 384 tests were conducted. Among them, 43 EMI events were observed. Collected results reveal that RF hand-held transmitters operating in these two frequency bands do not pose additional risk compared to pre-5G bands and that the safety distance of 15 cm typically indicted by the PM/ICD manufacturer is still able to guarantee the patient’s safety.
Smoking Bans and Circulatory System Disease Mortality Reduction in Macao (China): Using GRA Models
Authors: Peng Xinxin , Tang Xiaolei , Zhang Jing Hua , Chen Yijun
Published: March 2023

This study evaluates the association between smoking rates and mortality from circulatory system diseases (CSD) after implementing a series of smoking bans in Macao (China). (1) Background: Macao phased in strict total smoking bans since 2012. During the past decade, smoking rates among Macao women have dropped by half. CSD mortalities in Macao also show a declining trend. (2) Method: Grey relational analysis (GRA) models were adopted to rank the importance of some key factors, such as income per capita, physician density, and smoking rates. Additionally, regressions were performed with the bootstrapping method. (3) Results: Overall, smoking rate was ranked as the most important factor affecting CSD mortality among the Macao population. It consistently remains the primary factor among Macao’s female population. Each year, on average 5 CSD-caused deaths were avoided among every 100,000 women, equivalent to about 11.45% of the mean annual CSD mortality. (4) Conclusions: After the implementation of smoking bans in Macao, the decrease in smoking rate among women plays a primary role in the reduction in CSD mortality. To avoid excess CSD mortality due to smoking, Macao needs to continue to promote smoking cessation among the male population.
Analysis of Factors Affecting Academic Performance of Mathematics Education Doctoral Students: A Structural Equation Modeling Approach
Authors: Wijaya Tommy Tanu , Yu Boran , Xu Fei , Yuan Zhiqiang , Mailizar Mailizar
Published: March 2023

Student academic performance is an important indicator of doctoral education quality, but limited research has focused on how multiple influential factors of doctoral students’ academic performance work together. This study aims to explore the factors significantly affecting the academic performance of mathematics education doctoral students in Indonesia. Several factors were recognized from prior studies, such as the fear of delay, student engagement, parental support, teacher support, facilitating conditions, stress level, and well-being. An online questionnaire was designed and answered by a total of 147 mathematics education doctoral students. The partial least squares structural equation modeling (PLS-SEM) approach was adopted to analyze the questionnaire data. The results suggested that teacher support had the strongest positive effects on mathematics education doctoral students’ academic performance in Indonesia. Student engagement was the most significant positive factor in improving doctoral students’ well-being, while parental support could most significantly reduce their stress levels. Practically, these results are expected to provide implications to universities and supervisors regarding the improvement of doctoral students’ well-being to promote their academic success and further the quality of doctoral programs in education. Theoretically, these results can also contribute to building an empirical model that can be used to explore and explain how multiple factors could affect doctoral students’ academic performance in other contexts.
Effects of Whole-Body Vibration Exercise on Athletes with Ankle Instability: A Systematic Review
Authors: Coelho-Oliveira Ana Carolina , Taiar Redha , Pessanha-Freitas Juliana , Reis-Silva Aline , Ferreira-Souza Luiz Felipe , Jaques-Albuquerque Luelia Teles , Lennertz Aline , Moura-Fernandes Márcia Cristina , Rodrigues Lacerda Ana Cristina , Mendonça Vanessa A. , Sañudo Borja , Seixas Adérito , Boyer François Constant , Bernardo-Filho Mario , Rapin Amandine , Sá-Caputo Danúbia
Published: March 2023

Objective: Chronic Ankle Instability (CAI) or Functional Ankle Instability (FAI) is a condition characterized by laxity and mechanical instability in the ankle joint. This instability interferes with the activities and physical-functional parameters of athletes, which leads to repetitive ankle sprains. The current systematic review was carried out to identify the effects of whole-body vibration exercise (WBVE) in athletes with CAI. Methods: We conducted electronic searches in Pubmed, the Cochrane Library, Embase, Web of Science, Scopus, Science Direct, Allied Health Literature (CINAHL) and Academic Search Premier (ASP) (EBSCO) databases on 26 February 2022. Registers were identified, and studies were selected for inclusion according to the eligibility criteria. The methodological quality was assessed by the Physiotherapy Evidence Database (PEDro) scale. Results: Seven studies were included with a mean methodological quality score of 5.85, considered ‘regular’ quality on the PEDro scale. WBVE interventions in athletes with CAI showed that this exercise contributes to a better response on parameters of neuromuscular performance, muscle strength and consequently in balance and postural control, variables that are for the management of CAI. Conclusion: WBVE interventions in sports modalities promote physiological responses that may lead to positive effects in several parameters. The protocols proposed in each modality can be carried out in practice and are considered effective additional exercise and training methods beyond traditional types of training for athletes. However, more studies are needed on athletes with this condition, with specific protocols, to highlight the possible physiological and physical-functional responses. Protocol study registration: PROSPERO (CRD42020204434).