bluuebunny/crossref_metadata_2025 huggingface dataset:
Forensic DNA Phenotyping: Genes and Genetic Variants for Eye Color Prediction
Authors: Brancato Desiree , Coniglio Elvira , Bruno Francesca , Agostini Vincenzo , Saccone Salvatore , Federico Concetta
Published: August 2023

In recent decades, the use of genetic polymorphisms related to specific phenotypes, such as eye color, has greatly contributed to the development of the research field called forensic DNA phenotyping (FDP), enabling the investigators of crime cases to reduce the number of suspects, making their work faster and more precise. Eye color is a polygenic phenotype, and many genetic variants have been highlighted, with the major contributor being the HERC2-OCA2 locus, where many single nucleotide variations (SNPs) were identified. Interestingly, the HERC2-OCA2 locus, containing the intronic SNP rs12913832, the major eye color determinant, shows a high level of evolutionary conservation across many species of vertebrates. Currently, there are some genetic panels to predict eye color by genomic DNA analysis, even if the exact role of the SNP variants in the formation of eye color is still poorly understood, with a low level of predictivity in the so-called intermediate eye color. Many variants in OCA2, HERC2, and other genes lie in introns or correspond to synonymous variants, highlighting greater complexity in the mechanism of action of such genes than a simple missense variation. Here, we show the main genes involved in oculocutaneous pigmentation and their structural and functional features, as well as which genetic variants show the highest level of eye color predictivity in currently used FDP assays. Despite the great recent advances and impact of FDP in criminal cases, it is necessary to enhance scientific research to better understand the mechanism of action behind each genetic variant involved in eye color, with the goal of obtaining higher levels of prediction.
Exploring Communication Practices in Italian Physiotherapy: Knowledge and Use of Effective Communication Strategies—A National Descriptive Study
Authors: Al-Wardat Mohammad , Etoom Mohammad , Lena Francesco , Pellicciari Leonardo , D’Amone Francesco , Kossi Oyéné , Brindisino Fabrizio , Abdullahi Auwal
Published: August 2023

This study aimed to investigate the knowledge and use of effective communication strategies among Italian physiotherapists. We utilized a questionnaire consisting of 19 questions to collect data on the knowledge and use of effective communication strategies among Italian physiotherapists. The results revealed that only 35.8% of the respondents reported being aware of communication strategies related to physiotherapy, with their first exposure occurring during their three-year degree. Despite the majority of respondents agreeing that communication is an effective strategy for improving patient adherence, only about half reported making moderate use of open-ended questions and metaphors during treatment sessions. Furthermore, more than half of the respondents reported being unaware of Motivational Interviewing. The results of this study found that there is a consensus among Italian physiotherapists about the importance of effective communication in clinical practice, though the knowledge and application of some communication strategies remain limited. These findings suggest that there is room for improvement in the training and education of physiotherapists in Italy, with a need for greater emphasis on communication strategies in the university educational curriculum, starting from the bachelor’s degree.
The Impact of Upper Limb Apraxia on General and Domain-Specific Self-Efficacy in Post-Stroke Patients
Authors: Sánchez-Bermejo Laura , Milla-Ortega Pedro Jesús , Pérez-Mármol José Manuel
Published: August 2023

Background: Upper limb apraxia (ULA) is a neurological syndrome characterized by the inability to perform purposeful movements. ULA could impact individuals’ perceptions, including perceived self-efficacy. The aim of this study is to investigate whether ULA is related to general self-efficacy and self-efficacy for managing symptoms in post-stroke patients. Methods: A cross-sectional study was conducted involving 82 post-stroke patients. Regression analyses were implemented using a stepwise model including seven dimensions of ULA: imitation (non-symbolic, intransitive, and transitive), pantomime (non-symbolic, intransitive, and transitive), and dimension of apraxic performance in activities of daily living. These dimensions were independent variables, while general self-efficacy and symptom management self-efficacy dimensions were dependent variables. Results: The findings revealed that intransitive imitation accounted for 14% of the variance in general self-efficacy and 10% of self-efficacy for managing emotional symptoms. Transitive imitation explained 10% of the variance in self-efficacy for managing global symptoms and 5% for social–home integration symptoms. The combination of intransitive imitation, non-symbolic pantomime, and alterations in activities of daily living performance associated with ULA explained 24% of the variance in cognitive self-efficacy. Conclusions: Hence, ULA dimensions seem to be related to the levels of general perceived self-efficacy and self-efficacy for managing symptoms among post-stroke patients.
The Effects of Different Auxin–Cytokinin Combinations on Morphogenesis of Fritillaria meleagris Using Bulb Scale Sections In Vitro
Authors: Marković Marija , Trifunović-Momčilov Milana , Radulović Olga , Paunović Danijela M. , Antonić Reljin Dragana D. , Uzelac Branka , Subotić Angelina
Published: August 2023

Fritillaria meleagris is a horticulturally and medicinally valuable bulbous plant that requires a period of low temperatures for proper growth and flowering. Since conventional methods of propagation are ineffective and very slow, tissue culture techniques offer an integrated approach to mass production of this valuable geophyte. In this study, we investigated the effects of various auxin–cytokinin combinations on different morphogenetic pathways in bulb scale culture. Bulbs obtained in vitro were cut longitudinally, and bulb scales were cultured for four weeks at 7 °C on MS medium supplemented with 6-benzylaminopurine (BAP) in combination with 2,4-dichlorophenoxyacetic acid (2,4-D) or α-naphthaleneacetic acid (NAA) at different concentrations in order to investigate the influence of plant growth regulators (PGRs) on different morphogenetic responses. Regeneration percentage, number of shoots per explant, shoot length, number of bulbs and number of somatic embryos were monitored weekly. After chilling, bulb scales were transferred to 24 °C, and all parameters were recorded again. Low PGR concentrations were very effective for shoot multiplication, yielding up to 5.5 shoots per explant. 2,4-D (at 2 mg/L) in combination with low BAP (0.25 mg/L) produced the highest number of bulbs (11.00 ± 0.00), while PGR-free medium was extremely effective in somatic embryo formation (13.50 ± 2.90). Detached somatic embryos and bulblets continued to grow and develop on fresh PGR-free medium. We present data demonstrating that low auxin–cytokinin concentrations and PGR-free medium provide an effective method for a combined morphogenetic pathway in F. meleagris that is suitable for large-scale propagation.
The Biosynthesis, Mechanism of Action, and Physiological Functions of Melatonin in Horticultural Plants: A Review
Authors: Kumari Aradhna , Singh Santosh Kumar , Mathpal Bhupendra , Verma Krishan K. , Garg Vinod Kumar , Bhattacharyya Malini , Bhatt Rajan
Published: August 2023

Melatonin, a hormone known for its role in regulating sleep–wake cycles in mammals, has been found to have diverse functions in horticultural plants. In recent years, research has revealed the involvement of melatonin in various physiological processes in plants, like regulation of growth and development, stress tolerance, and antioxidant defense. Melatonin can augment seed germination, roots, shoot growth, and biomass accumulation in horticultural crops. It also performs a vital role in regulating vegetative and reproductive growth stages, floral transition, and leaf senescence. Melatonin improves stress tolerance in crops by regulating root architecture, nutrient uptake, and ion transport. Additionally, melatonin works like a broad-spectrum antioxidant by scavenging reactive oxygen species and enhancing antioxidant activity. The mechanism of action of melatonin in horticultural plants involves gene expressions, hormone signaling pathways, and antioxidant defense pathways. Melatonin also interacts with other plant growth regulators (PGRs), comprising auxins, cytokinins, and abscisic acid to coordinate various physiological processes in plants. Melatonin has evolved as a versatile chemical entity with diverse functions in horticultural plants, and its potential applications in crop production and stress management are increasingly being explored. This review aims to provide a comprehensive insight into the present state of knowledge about melatonin and its role in horticulturally important plants and identify avenues for further research and practical applications. Further study must be conducted to fully elucidate the mechanisms of melatonin action in crops and to outline effective strategies for its practical use in horticultural practices.
The Burden of Pediatric Visual Impairment and Ocular Diagnoses in Barbados
Authors: Da Silva Kirsten , Dowell Michelle , Savatovsky Eleonore J. , Grosvenor Dawn , Callender David , Campbell Michael H. , Hambleton Ian , Vanner Elizabeth A. , Grajewski Alana L. , Chang Ta Chen
Published: August 2023

Visual impairment (VI) negatively affects a child’s quality of life. The prevalence of VI in the Caribbean is nearly three times higher than in the United States, but the causes remain uncertain. This study leverages Barbados’ unique eye care system to survey the eye diseases and VI prevalence in Barbadian children. Medical records of all patients aged <19 years who received ophthalmic care in Barbados’ two public eye care centers between January and December 2019 were reviewed, capturing the entirety of public pediatric eye care within the study period. Age at the first visit to the clinic and at the final visit in 2019, sex, best-corrected visual acuity (BCVA), past medical history, and clinical diagnoses were extracted and analyzed. VI was defined as a BCVA of 6/12 or worse in the better-seeing eye. There were 3278 patient records with a mean age at the first visit of 7.8 ± 3.9 years. There were 80 (2.4%) children with VI, 62.5% of which were attributed to amblyopia. A total of 94% of VI was preventable or treatable. The most common diagnoses were refractive error (87.5%), strabismus (27.5%), and allergic eye disease (20.0%). Amblyopia is the major cause of pediatric VI in Barbados and is largely avoidable.
Nexus of Financing Constraints and Supply Chain Finance: Evidence from Listed SMEs in China
Authors: Ng Sin-Huei , Yang Yunze , Lee Chin-Chong , Ong Chui-Zi
Published: August 2023

As opposed to developed markets, financing constraints are a more pressing issue among Small and Medium-Sized Enterprises (SMEs) in emerging markets. We explore the severity of financing constraints on SMEs, and examine the role of supply chain finance (SCF) in alleviating those constraints, with the focus on a large emerging market: China. Using the panel data of SMEs listed on Shenzhen Stock Exchange from 2014 to 2020, we employ robust estimations of panel-corrected standard errors (PCSEs) and robust fixed-effects methods to analyze the issue. Our cash–cash-flow sensitivity model points out that listed SMEs in China show significant cash–cash-flow sensitivity, and financing constraints are prevalent. We document that the development of SCF has a mitigation effect on the financing constraints on the SMEs. Our robustness test with Yohai’s MM-estimator is also supportive of the main finding. Our study indicates the importance of supply chain finance development in alleviating the financing constraints on SMEs and, subsequently, supporting their sustainability journey. Overall, our findings have important policy implications for the stakeholders involved in emerging markets, and there are lessons to be learned from the Chinese experience. There is still much to be explored in the nexus of SCF and the financing difficulties of SMEs in China at present, with much of the extant literature concentrating only on specific financing mechanisms. Thus, our study fills the gap by providing a broad and comprehensive analysis of the issue.
Beyond Motor Deficits: Environmental Enrichment Mitigates Huntington’s Disease Effects in YAC128 Mice
Authors: Plácido Evelini , Gomes Welter Priscilla , Wink Ana , Karasiak Gabriela Duarte , Outeiro Tiago Fleming , Dafre Alcir Luiz , Gil-Mohapel Joana , Brocardo Patricia S.
Published: August 2023

Huntington’s disease (HD) is a neurodegenerative genetic disorder characterized by motor, psychiatric, cognitive, and peripheral symptoms without effective therapy. Evidence suggests that lifestyle factors can modulate disease onset and progression, and environmental enrichment (EE) has emerged as a potential approach to mitigate the progression and severity of neurodegenerative processes. Wild-type (WT) and yeast artificial chromosome (YAC) 128 mice were exposed to different EE conditions. Animals from cohort 1 were exposed to EE between postnatal days 21 and 60, and animals from cohort 2 were exposed to EE between postnatal days 60 and 120. Motor and non-motor behavioral tests were employed to evaluate the effects of EE on HD progression. Monoamine levels, hippocampal cell proliferation, neuronal differentiation, and dendritic arborization were also assessed. Here we show that EE had an antidepressant-like effect and slowed the progression of motor deficits in HD mice. It also reduced monoamine levels, which correlated with better motor performance, particularly in the striatum. EE also modulated neuronal differentiation in the YAC128 hippocampus. These results confirm that EE can impact behavior, hippocampal neuroplasticity, and monoamine levels in YAC128 mice, suggesting this could be a therapeutic strategy to modulate neuroplasticity deficits in HD. However, further research is needed to fully understand EE’s mechanisms and long-term effects as an adjuvant therapy for this debilitating condition.
The Role of Specialized Pro-Resolving Lipid Mediators in Inflammation-Induced Carcinogenesis
Authors: Torres Wheeler , Pérez José Luis , Díaz María P. , D’Marco Luis , Checa-Ros Ana , Carrasquero Rubén , Angarita Lissé , Gómez Yosselin , Chacín Maricarmen , Ramírez Paola , Villasmil Nelson , Durán-Agüero Samuel , Cano Clímaco , Bermúdez Valmore
Published: August 2023

Cancer is a process involving cell mutation, increased proliferation, invasion, and metastasis. Over the years, this condition has represented one of the most concerning health problems worldwide due to its significant morbidity and mortality. At present, the incidence of cancer continues to grow exponentially. Thus, it is imperative to open new avenues in cancer research to understand the molecular changes driving DNA transformation, cell-to-cell interaction derangements, and immune system surveillance decay. In this regard, evidence supports the relationship between chronic inflammation and cancer. In light of this, a group of bioactive lipids derived from polyunsaturated fatty acids (PUFAs) may have a position as novel anti-inflammatory molecules known as the specialized pro-resolving mediators (SPMs), a group of pro-resolutive inflammation agents that could improve the anti-tumor immunity. These molecules have the potential role of chemopreventive and therapeutic agents for various cancer types, and their effects have been documented in the scientific literature. Thus, this review objective centers around understanding the effect of SPMs on carcinogenesis and their potential therapeutic effect.
A Novel Aniline Derivative from Peganum harmala L. Promoted Apoptosis via Activating PI3K/AKT/mTOR-Mediated Autophagy in Non-Small Cell Lung Cancer Cells
Authors: Wu Zhongnan , Li Wen , Tang Qing , Huang Laiqiang , Zhan Zhaochun , Li Yaolan , Wang Guocai , Dai Xiaoyong , Zhang Yubo
Published: August 2023

Non-small cell lung cancer (NSCLC) is a common clinical malignant tumor with limited therapeutic drugs. Leading by cytotoxicity against NSCLC cell lines (A549 and PC9), bioactivity-guided isolation of components from Peganum harmala seeds led to the isolation of pegaharoline A (PA). PA was elucidated as a structurally novel aniline derivative, originating from tryptamine with a pyrrole ring cleaved and the degradation of carbon. Biological studies showed that PA significantly inhibited NSCLC cell proliferation, suppressed DNA synthesis, arrested the cell cycle, suppressed colony formation and HUVEC angiogenesis, and blocked cell invasion and migration. Molecular docking and surface plasmon resonance (SPR) demonstrated PA could bind with CD133, correspondingly decreased CD133 expression to activate autophagy via inhibiting the PI3K/AKT/mTOR pathway, and increased ROS levels, Bax, and cleaved caspase-3 to promote apoptosis. PA could also decrease p-cyclinD1 and p-Erk1/2 and block the EMT pathway to inhibit NSCLC cell growth, invasion, and migration. According to these results, PA could inhibit NSCLC cell growth by blocking PI3K/AKT/mTOR and EMT pathways. This study provides evidence that PA has a promising future as a candidate for developing drugs for treating NSCLC.
Enhancement of Arc Erosion Resistance in AgCuO Electrical Contact Materials through Rare Earth Element Doping: First-Principles and Experimental Studies
Authors: Wang Haitao , Wang Yanling , Wang Jingqin , Cai Qinglong , Hu Dekao
Published: August 2023

To investigate the stability and electrical and physical properties of undoped CuO and CuO doped with rare earth elements, electronic structures and elastic constants were calculated using first-principles density functional theory. Additionally, experimental verification was carried out on AgCuO and AgCuO-X (La, Ce, Y) electrical contacts, which were prepared using sol–gel and powder metallurgy methods. The contacts were tested under an 18 V/15 A DC resistive load using the JF04D contact material testing system. Arc parameters were analyzed, and three-dimensional surface profilometry and scanning electron microscopy were used to study the altered erosion morphology of the electrically contacted materials; moreover, the potential mechanisms behind their arc erosion behavior were investigated in depth. The results demonstrate that the doping of rare earth elements can improve the electrical conductivity and physical properties of the contacts, optimize the arc parameters, and enhance their resistance to arc erosion. Notably, AgCuO-Ce exhibited the highest electrical conductivity and the least amount of material transfer; moreover, it had excellent arc time and energy parameters, resulting in the best resistance to arc erosion. This study provides a theoretical basis for the screening of doping elements to enhance the performance of AgCuO contact materials and offers new ideas and scientific references for this field.
Smouldering Lesion in MS: Microglia, Lymphocytes and Pathobiochemical Mechanisms
Authors: Pukoli Dániel , Vécsei László
Published: August 2023

Multiple sclerosis (MS) is an immune-mediated, chronic inflammatory, demyelinating, and neurodegenerative disease of the central nervous system (CNS). Immune cell infiltration can lead to permanent activation of macrophages and microglia in the parenchyma, resulting in demyelination and neurodegeneration. Thus, neurodegeneration that begins with acute lymphocytic inflammation may progress to chronic inflammation. This chronic inflammation is thought to underlie the development of so-called smouldering lesions. These lesions evolve from acute inflammatory lesions and are associated with continuous low-grade demyelination and neurodegeneration over many years. Their presence is associated with poor disease prognosis and promotes the transition to progressive MS, which may later manifest clinically as progressive MS when neurodegeneration exceeds the upper limit of functional compensation. In smouldering lesions, in the presence of only moderate inflammatory activity, a toxic environment is clearly identifiable and contributes to the progressive degeneration of neurons, axons, and oligodendrocytes and, thus, to clinical disease progression. In addition to the cells of the immune system, the development of oxidative stress in MS lesions, mitochondrial damage, and hypoxia caused by the resulting energy deficit and iron accumulation are thought to play a role in this process. In addition to classical immune mediators, this chronic toxic environment contains high concentrations of oxidants and iron ions, as well as the excitatory neurotransmitter glutamate. In this review, we will discuss how these pathobiochemical markers and mechanisms, alone or in combination, lead to neuronal, axonal, and glial cell death and ultimately to the process of neuroinflammation and neurodegeneration, and then discuss the concepts and conclusions that emerge from these findings. Understanding the role of these pathobiochemical markers would be important to gain a better insight into the relationship between the clinical classification and the pathomechanism of MS.
Bacteriocin-Producing Escherichia coli Q5 and C41 with Potential Probiotic Properties: In Silico, In Vitro, and In Vivo Studies
Authors: Mihailovskaya Veronika S. , Sutormin Dmitry A. , Karipova Marina O. , Trofimova Anna B. , Mamontov Victor A. , Severinov Konstantin , Kuznetsova Marina V.
Published: August 2023

Commensal bacteriocin-producing Escherichia coli are of interest for possible use as probiotics to selectively control the spread of pathogenic bacteria. Here, we evaluated the biosafety and efficacy of two new bacteriocin-producing E. coli strains, Q5 (VKM B-3706D) and C41 (VKM B-3707D), isolated from healthy farm animals. The genomes of both strains were sequenced, and genes responsible for the antagonistic and colonization abilities of each strain were identified. In vitro studies have shown that both strains were medium-adhesive and demonstrated antagonistic activity against most enteropathogens tested. Oral administration of 5 × 108 to 5 × 1010 colony-forming units of both strains to rats with drinking water did not cause any disease symptoms or side effects. Short-term (5 days) oral administration of both strains protected rats from colonization and pathogenic effects of a toxigenic beta-lactam-resistant strain of E. coli C55 and helped preserve intestinal homeostasis. Taken together, these in silico, in vitro, and in vivo data indicate that both strains (and especially E. coli Q5) can be potentially used for the prevention of colibacillosis in farm animals.
Characterization of GEXP15 as a Potential Regulator of Protein Phosphatase 1 in Plasmodium falciparum
Authors: Mansour Hala , Cabezas-Cruz Alejandro , Peucelle Véronique , Farce Amaury , Salomé-Desnoulez Sophie , Metatla Ines , Guerrera Ida Chiara , Hollin Thomas , Khalife Jamal
Published: August 2023

The Protein Phosphatase type 1 catalytic subunit (PP1c) (PF3D7_1414400) operates in combination with various regulatory proteins to specifically direct and control its phosphatase activity. However, there is little information about this phosphatase and its regulators in the human malaria parasite, Plasmodium falciparum. To address this knowledge gap, we conducted a comprehensive investigation into the structural and functional characteristics of a conserved Plasmodium-specific regulator called Gametocyte EXported Protein 15, GEXP15 (PF3D7_1031600). Through in silico analysis, we identified three significant regions of interest in GEXP15: an N-terminal region housing a PP1-interacting RVxF motif, a conserved domain whose function is unknown, and a GYF-like domain that potentially facilitates specific protein–protein interactions. To further elucidate the role of GEXP15, we conducted in vitro interaction studies that demonstrated a direct interaction between GEXP15 and PP1 via the RVxF-binding motif. This interaction was found to enhance the phosphatase activity of PP1. Additionally, utilizing a transgenic GEXP15-tagged line and live microscopy, we observed high expression of GEXP15 in late asexual stages of the parasite, with localization predominantly in the nucleus. Immunoprecipitation assays followed by mass spectrometry analyses revealed the interaction of GEXP15 with ribosomal- and RNA-binding proteins. Furthermore, through pull-down analyses of recombinant functional domains of His-tagged GEXP15, we confirmed its binding to the ribosomal complex via the GYF domain. Collectively, our study sheds light on the PfGEXP15–PP1–ribosome interaction, which plays a crucial role in protein translation. These findings suggest that PfGEXP15 could serve as a potential target for the development of malaria drugs.
Insights into Hepatocellular Carcinoma in Patients with Thalassemia: From Pathophysiology to Novel Therapies
Authors: Lin Pei-Chin , Hsu Wan-Yi , Lee Po-Yi , Hsu Shih-Hsien , Chiou Shyh-Shin
Published: August 2023

Thalassemia is a heterogeneous congenital hemoglobinopathy common in the Mediterranean region, Middle East, Indian subcontinent, and Southeast Asia with increasing incidence in Northern Europe and North America due to immigration. Iron overloading is one of the major long-term complications in patients with thalassemia and can lead to organ damage and carcinogenesis. Hepatocellular carcinoma (HCC) is one of the most common malignancies in both transfusion-dependent thalassemia (TDT) and non-transfusion-dependent thalassemia (NTDT). The incidence of HCC in patients with thalassemia has increased over time, as better chelation therapy confers a sufficiently long lifespan for the development of HCC. The mechanisms of iron-overloading-associated HCC development include the increased reactive oxygen species (ROS), inflammation cytokines, dysregulated hepcidin, and ferroportin metabolism. The treatment of HCC in patients with thalassemia was basically similar to those in general population. However, due to the younger age of HCC onset in thalassemia, regular surveillance for HCC development is mandatory in TDT and NTDT. Other supplemental therapies and experiences of novel treatments for HCC in the thalassemia population were also reviewed in this article.
Inhibition of the Exocyst Complex Attenuates the LRRK2 Pathological Effects
Authors: Ciampelli Cristina , Galleri Grazia , Puggioni Silvia , Fais Milena , Iannotta Lucia , Galioto Manuela , Becciu Marta , Greggio Elisa , Bernardoni Roberto , Crosio Claudia , Iaccarino Ciro
Published: August 2023

Pathological mutations in leucine-rich repeat kinase 2 (LRRK2) gene are the major genetic cause of Parkinson’s disease (PD). Multiple lines of evidence link LRRK2 to the control of vesicle dynamics through phosphorylation of a subset of RAB proteins. However, the molecular mechanisms underlying these processes are not fully elucidated. We have previously demonstrated that LRRK2 increases the exocyst complex assembly by Sec8 interaction, one of the eight members of the exocyst complex, and that Sec8 over-expression mitigates the LRRK2 pathological effect in PC12 cells. Here, we extend this analysis using LRRK2 drosophila models and show that the LRRK2-dependent exocyst complex assembly increase is downstream of RAB phosphorylation. Moreover, exocyst complex inhibition rescues mutant LRRK2 pathogenic phenotype in cellular and drosophila models. Finally, prolonged exocyst inhibition leads to a significant reduction in the LRRK2 protein level, overall supporting the role of the exocyst complex in the LRRK2 pathway. Taken together, our study suggests that modulation of the exocyst complex may represent a novel therapeutic target for PD.
Comparison of Fine Structure of the Compound Eyes in Eucryptorrhynchus scrobiculatus and Eucryptorrhynchus brandti Adults
Authors: Hao Yingying , Wang Qi , Wen Chao , Wen Junbao
Published: August 2023

Eucryptorrhynchus scrobiculatus and E. brandti are the main borers of Ailanthus altissima, causing serious economic and ecological losses. The external morphology and internal ultrastructure of the compound eyes of two related weevils were investigated with light microscopy, scanning electron microscopy, and transmission electron microscopy. E. scrobiculatus and E. brandti possess a pair of reniform apposition compound eyes and contain about 550 ommatidia per eye. The interommatidial angle of E. scrobiculatus and E. brandti are 7.08 ± 0.31° and 4.84 ± 0.49°, respectively. The corneal thickness, rhabdom length, and ommatidium length of E. scrobiculatus are significantly greater than those of E. brandti. Under light-adapted conditions, the pigment granules are mainly distributed at the junction of the cone and the rhabdom, and the diameter and the cross-sectional area of the middle end of the rhabdom is increased in the two weevil species. Under dark-adapted conditions, the pigment granules shift longitudinally and are evenly distributed on both sides of the cone and the rhabdom, and the diameter and cross-sectional area of the middle end of the rhabdom are decreased. The discrepancy in visual structure is beneficial for adaptation to niche differentiation of the two related species. The present results suggest that the two weevils possess different visual organ structures to perceive visual information in the external environment.
Evaluation of a New Treatment Strategy for Geriatric Fragility Fractures of the Posterior Pelvic Ring Using Sensor-Supported Insoles: A Proof-of-Concept Study
Authors: Lebert Luca , Keppler Alexander Martin , Bruder Jan , Faust Leon , Becker Christopher Alexander , Böcker Wolfgang , Neuerburg Carl , Cavalcanti Kußmaul Adrian
Published: August 2023

The incidence of fragility fractures of the pelvis (FFPs) is currently rising. Surgical treatment, which is performed using sacroiliac screws, is complicated by compromised bone quality, oftentimes resulting in implant failure. The iFuse implant system aims to improve attachment and durability with promising results for sacroiliac dysfunction, though data for its feasibility on FFPs are rare. Consequently, this study aims to evaluate the feasibility of the iFuse for FFPs. A total of 10 patients with FFPs were treated with the iFuse in this study. Pre- and postoperatively, both mobility using an established insole force sensor for an inpatient gait analysis and general well-being and pain using questionnaires were evaluated. When comparing pre- and postoperative findings, this study demonstrated a significant increase in the average (8.14%) and maximum (9.4%) loading (p < 0.001), a reduction in pain, as measured by the visual analog scale (VAS), from 4.60 to 2.80 at rest (p = 0.011) and from 7.00 to 4.40 during movement (p = 0.008), an increase in the Barthel Index by 20 points (p < 0.001) and an increase in the Parker Mobility Score by 2.00 points (p = 0.011). All this contributes to the possibility of early postoperative mobilization and improved general well-being, ultimately preventing the late consequences of postoperative immobilization and maintaining patients autonomy and contentment.
Internet Addiction and Physical Activity among Polish and Portuguese Students in the Final Year of the COVID-19 Pandemic
Authors: Zalewska Anna , Gałczyk Monika , Sobolewski Marek , Fernandes Hélder
Published: August 2023

Aim: The purpose of this study was to analyze the level of Internet addiction and physical activity, as well as the relationship between internet addiction and physical activity, among Polish and Portuguese students in the final year of the COVID-19 pandemic. Methods: A web-based online survey was conducted among students in Poland and Portugal (398 respondents) aged between 17 and 26 in October 2022. The level of internet addiction was assessed by the Kimberly Young questionnaire in Polish and in Portuguese. The level of physical activity was measured by the international physical activity questionnaire in Polish and in Portuguese. Results: In the study population, the majority of the respondents were mildly addicted to the internet. The average level of addiction was reported by 9.3–23.5% of the respondents. The average level of internet addiction was higher among Portuguese students and among men. Those who had suffered from COVID-19 (especially more than once) and were more physically active showed a higher tendency towards internet addiction. Conclusions: Internet addiction is a problem that has to be monitored and given more attention, particularly among male students. The results presented provide the first statistical insight into the problem of internet addiction among students in both countries and form the basis for further studies. They also highlight the severity of the problem and point to the need for early preventive and protective interventions against problematic internet use. It appears that effective strategies should include promoting the benefits of physical activity.
Treatment of Myopia with Atropine 0.125% Once Every Night Compared with Atropine 0.125% Every Other Night: A Pilot Study
Authors: Chen Zi-Rong , Chen Shin-Chieh , Wan Tsung-Yao , Chuang Lan-Hsin , Chen Hung-Chi , Yeh Lung-Kun , Kuo Yu-Kai , Wu Pei-Chang , Chen Yun-Wen , Lai Ing-Chou , Hwang Yih-Shiou , Liu Chun-Fu
Published: August 2023

(1) Purpose: To investigate the efficacy of myopia treatment in children using atropine 0.125% once every two nights (QON) compared with atropine 0.125% once every night (HS). (2) Methods: This retrospective cohort study reviewed the medical records of two groups of children with myopia. Group 1 comprised children treated with atropine 0.125% QON, while group 2 included children treated with atropine 0.125% HS. The first 6 months of data of outcome measurements were subtracted as washout periods in those children undergoing both atropine QON and HS treatment. The independent t-test and Pearson’s chi-square test were used to compare the baseline clinical characteristics between the two groups. A generalized estimating equations (GEE) model was used to determine the factors that influence treatment effects. (3) Results: The average baseline ages of group 1 (38 eyes from 19 patients) and group 2 (130 eyes from 65 patients) were 10.6 and 10.2 years, respectively. There were no significant differences in axial length (AL) or cycloplegic spherical equivalent (SEq) at baseline or changes of them after 16.9 months of follow-up. GEE showed that the frequency of atropine 0.125% use has no association with annual AL (QON vs. HS: 0.16 ± 0.10 vs. 0.18 ± 0.12) and SEq (QON vs. HS: −0.29 ± 0.44 vs. −0.34 ± 0.36) changes in all children with myopia. It also showed that older baseline age (B = −0.020, p < 0.001) was associated with lesser AL elongation. (4) Conclusion: The treatment effects of atropine 0.125% HS and QON were similar in this pilot study. The use of atropine 0.125% QON may be an alternative strategy for children who cannot tolerate the side effects of atropine 0.125% HS. This observation should be confirmed with further large-scale studies.
Abundance, Biomass, and Production of Bacterioplankton at the End of the Growing Season in the Western Laptev Sea: Impact of Khatanga River Discharge (Arctic)
Authors: Kopylov Alexander I. , Kosolapov Dmitriy B. , Romanenko Anna V. , Zabotkina Elena A. , Sazhin Andrey F.
Published: August 2023

The structure and productivity of planktonic microbial communities in the ecosystems of the Siberian Arctic seas are significantly dependent on freshwater input. During the study, we determined the spatial distribution of the abundance, biomass, and production of heterotrophic bacterioplankton in the Western Laptev Sea on the transect from the Khatanga River estuary to the continental slope and assessed the impact of river freshwater discharge. The influence of fresh water on bacterioplankton was restricted mainly to Khatanga Bay (KHAB) and the transitional zone (TZ) and was poorly recognized in the Western shelf (WS) and continental slope (CS) areas. The total bacterial abundance decreased from KHAB to the CS. Particle-attached bacteria constituted on average 63.0% of the total abundance of bacterioplankton in KHAB and 1.0% at the CS. Average bacterial production in the water column was highest in KHAB (10.3 mg C m−3 d−1), decreasing towards the CS (0.7 mg C m−3 d−1). In KHAB and TZ, bacteria were the main component of the planktonic community (44−55%). These results show that at the end of the growing season, bacterial processes prevailed over autotrophic ones and contributed largely to the total biological carbon flux in the coastal ecosystem of the Western Laptev Sea.
Field Measurements of Longshore Sediment Transport along Denu Beach, Volta Region, Ghana
Authors: Agbetossou Kwami Serge Foga , Aheto Denis Worlanyo , Angnuureng Donatus Bapentire , van Rijn Leo Cornelius , Houédakor Koko Zébéto , Brempong Emmanuel Kwadzo , Tomety Folly Serge
Published: August 2023

Field measurements of longshore sediment transport have been performed in the surf zone along Denu Beach, located in the Volta Region of Ghana, West Africa. This study consisted of measuring sediment transport rates by the deployment of modified versions of Kraus streamer traps on a cross-shore distance, which was about half the average surf zone width. In addition to the measured transport rates, data on waves, longshore currents, and other morphological parameters were collected with simple and low-cost instrumentation. In total, about 22 datasets were obtained through several days of data collection spanning a period of 5 months. Mathematical formulae proposed by CERC (1984), Kamphuis (1991), and the new van Rijn (2014) were used to compute theoretical transports for the entire surf zone, which were validated by measured data. Applying the CERC equation with a K-value of 0.39, the theoretical transports were about one order of magnitude higher than the measured rates. The rates computed by the Kamphuis formula were slightly higher than those yielded by the van Rijn equation. They produced values that were, on average, about 3.5 and 2.9 times higher than the measured rates, respectively. This study then confirmed the capabilities of both the Kamphuis and van Rijn equations to provide closer estimates of longshore sediment transport on Denu Beach. It especially sheds light on the new van Rijn formula, which has not been used extensively in LST quantification across the world. We, therefore, concluded that both the Kamphuis and van Rijn equations could be particularly useful to local engineers in the prediction of LST prior to the design of coastal protection structures. Further long-term studies employing available cutting-edge technologies were also recommended to provide more information on longshore transport not only in Ghana but also in the entire Gulf of Guinea and for the establishment of a reliable sediment budget for the country and the entire region.
Effectiveness of Dupilumab in the Treatment of Adult and Older Adult Patients with Severe, Uncontrolled CRSwNP
Authors: Ottaviano Giancarlo , De Corso Eugenio , Saccardo Tommaso , D’Auria Leandro Maria , Zampollo Sonny , D’Agostino Giuseppe , Mairani Edoardo , De Maio Gabriele , Scarpa Bruno , Bacci Christian , Favero Vittorio
Published: August 2023

Chronic rhinosinusitis with nasal polyps (CRSwNP) is a multifactorial disease that significantly impacts patients’ quality of life. New therapeutic strategies and in particular biologic treatments are now available for these patients. It has been demonstrated that Dupilumab (an anti IL-4/IL-13 biologic drug) is effective in reducing the size of nasal polyps and in improving patients’ symptoms and thus, quality of life. No real-world studies examining Dupilamab’s efficacy in the elderly with respect to other adult age groups have as yet been carried out. The aim of this multicentric study was to evaluate Dupilumab’s efficacy in young–middle adults as opposed to an older adult population affected by severe, uncontrolled CRSwNP. Of the 96 patients included in the study, 22 were 65 years old or older. Significant improvements were observed in all the parameters considered in both age groups after treatment was begun (T0 mean values for SNOT-22 = 58.5 ± 20.3, VAS NO = 7.6 ± 2.2, VAS smell = 8.6 ± 2.1, NPS = 5.6 ± 1.4, PNIF = 101.6 ± 59.4, S’S = 5.1 ± 3.1), T4 mean values for SNOT-22 = 15.1 ± 12.7, VAS NO = 1.7 ± 1.8, VAS smell = 2.4 ± 3, NPS = 1.7 ± 1.7, PNIF = 162.4 ± 43.2, S’S = 10.4 ± 3.7) (p < 0.0001). No differences in the variables considered were observed between the two age groups during the study, with the exception of the Peak Nasal Inspiratory Flow (PNIF), which was marginally higher; this was also the case according to multivariate analyses (p = 0.008) in the young–middle adult group with respect to the elderly one (p = 0.07). At multivariate analyses, asthma and the female sex negatively influenced the PNIF values (p = 0.001 and p = 0.012, respectively). Age negatively influenced the Visual Analog Scale (VAS) for nasal obstruction (p = 0.0032) and Endoscopic Sinus Surgery (ESS) negatively influenced the patents’ olfactory performance (p = 0.028) to the same degree in both groups. Dupilumab was found to be effective to the same degree in both age groups. It can be considered a safe and reliable option for the treatment of elderly patients with severe, uncontrolled CRSwNP.
Non-Invasive Estimation of Central Systolic Blood Pressure by Radial Tonometry: A Simplified Approach
Authors: Chemla Denis , Agnoletti Davide , Jozwiak Mathieu , Zhang Yi , Protogerou Athanase D. , Millasseau Sandrine , Blacher Jacques
Published: August 2023

Backround. Central systolic blood pressure (cSBP) provides valuable clinical and physiological information. A recent invasive study showed that cSBP can be reliably estimated from mean (MBP) and diastolic (DBP) blood pressure. In this non-invasive study, we compared cSBP calculated using a Direct Central Blood Pressure estimation (DCBP = MBP2/DBP) with cSBP estimated by radial tonometry. Methods. Consecutive patients referred for cardiovascular assessment and prevention were prospectively included. Using applanation tonometry with SphygmoCor device, cSBP was estimated using an inbuilt generalized transfer function derived from radial pressure waveform, which was calibrated to oscillometric brachial SBP and DBP. The time-averaged MBP was calculated from the radial pulse waveform. The minimum acceptable error (DCBP-cSBP) was set at ≤5 (mean) and ≤8 mmHg (SD). Results. We included 160 patients (58 years, 54%men). The cSBP was 123.1 ± 18.3 mmHg (range 86–181 mmHg). The (DCBP-cSBP) error was −1.4 ± 4.9 mmHg. There was a linear relationship between cSBP and DCBP (R2 = 0.93). Forty-seven patients (29%) had cSBP values ≥ 130 mmHg, and a DCBP value > 126 mmHg exhibited a sensitivity of 91.5% and specificity of 94.7% in discriminating this threshold (Youden index = 0.86; AUC = 0.965). Conclusions. Using the DCBP formula, radial tonometry allows for the robust estimation of cSBP without the need for a generalized transfer function. This finding may have implications for risk stratification.
“Emerging Topics in Pain Medicine”: Advancing Research and Patient-Centered Health Strategies
Authors: Cascella Marco , Petrucci Emiliano , Marinangeli Franco , Vittori Alessandro
Published: August 2023

Pain, in all its various forms and manifestations, impacts the lives of millions worldwide [...]
Bowel Perforation in Vascular Ehlers–Danlos Syndrome: Case Report and Comprehensive Review
Authors: Menni Alexandra , Tzikos Georgios , Sarafis Alexandros , Ververi Athina , Chatziantoniou George , Rafailidis Vasileios , Panidis Stavros , Goulas Patroklos , Karlafti Eleni , Apostolidis Stylianos , Giouleme Olga , Michalopoulos Antonios , Paramythiotis Daniel
Published: August 2023

Introduction: Ehlers–Danlos syndromes (EDS) comprise a rare variety of genetic disorders, affecting all types of collagen. Herein, we describe a case of the vascular type of EDS, with coexisting segmental absence of intestinal musculature, while simultaneously performing a narrative review of the existing literature. Case Presentation: A 23-year-old male patient with a history of multiple abdominal operations due to recurrent bowel perforations and the presence of a high-output enterocutaneous fistula was admitted to our surgical department for further evaluation and treatment. After detailed diagnostic testing, the diagnosis of vascular-type EDS (vEDS) was made and a conservative therapeutic approach was adopted. In addition, a comprehensive review of the international literature was carried out by applying the appropriate search terms. Results: The diagnosis of vEDS was molecularly confirmed by means of genetic testing. The patient was treated conservatively, with parenteral nutrition and supportive methods. Thirty-four cases of bowel perforation in vEDS have been reported so far. Interestingly, this case is the second one ever to report co-existence of vEDS with Segmental Absence of Intestinal Musculature. Conclusions: Establishing the diagnosis of vEDS promptly is of vital significance in order to ensure that patients receive appropriate treatment. Due to initial non-specific clinical presentation, EDS should always be included in the differential diagnoses of young patients with unexplained perforations of the gastrointestinal tract.
Insurance Penetration and Institutional Spillover on Economic Growth: A Dynamic Spatial Econometric Approach on the Asian and Europe Region
Authors: Fernando Kurukulasuriya Dinesh Udana Devindra , Tharanga Thambawita Maddumage Nimali , Dewasiri Narayanage Jayantha , Sood Kiran , Grima Simon , Thalassinos Eleftherios
Published: August 2023

The contemporary environment is interrelated, and interactions between markets, countries, and international actors at different levels exist in every corner of the globe. Amid this, the failures of the free-market system have paved the way for institutionalism, which proposes minimising transaction costs, substantial property rights, and enabling proper contract enforcement. Studies on institutions and insurance development spillover concerning growth relationships are rare and a critical area needing exploration. This study explores the behaviour of economic development in terms of potential spatial dependencies and spatial institutional and insurance development spillover on economic growth. To measure insurance development by the life insurance and non-life insurance penetration, economic growth by per capita gross domestic product (GDP), and indicators of good governance for institutions in the nations. The study explored the spatial impact between countries using panel data of 56 countries between 2002 and 2020 representing the Asian and European regions. We did this by using dynamic spatial econometric modelling (DSEM) on institutional and insurance development and seeing the spatial implications and the spatial institutional impact moderated by insurance development on growth. Results indicate that developing the life insurance and non-life insurance of surrounding countries creates a spillover impact on the local countries’ economies. In contrast, institutions have created a reverse spatial spillover impact on local countries. However, life insurance development, moderated through accountability and government effectiveness, has created a spatial spillover between countries. Both life and non-life penetration moderated by the control of corruption and overall institutions have shown a reverse spillover on countries’ economies. This suggests that global governance is a positive-sum game, and monitoring and governance structures have failed at the international level concerning separate countries. Therefore, it is seen that to prevent institutional failure at the state level, good governance and links with the global governance structure could disrupt or energise local institutions.