INFLUENCE OF ALCOHOL CONSUMPTION ON SP-A AND SP-D SURFACTANT PROTEIN IN NOVOSIBIRSK MEN AND WOMEN
Authors:   Research Institute of Therapy and Preventive Medicine - branch of the Federal State Budgetary Scientific Institution ", Nikolaev Konstantin Yurievich ,   Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences",   , Novosibirsk Russian Federation, Kosarev Ilya Aleksandrovich ,   Research Institute of Therapy and Preventive Medicine - branch of the Federal State Budgetary Scientific Institution "Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences", Novosibirsk Russian Federation, Dadashova Nazli Farhad Kyzy ,   Research Institute of Therapy and Preventive Medicine - branch of the Federal State Budgetary Scientific Institution "Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences", Novosibirsk Russian Federation, Lapitskaya Yana Konstantinovna ,   Research Institute of Therapy and Preventive Medicine - branch of the Federal State Budgetary Scientific Institution "Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences", Novosibirsk Russian Federation
Published: October 2023

Introduction. Currently, there are a few experimental data on the effect of alcohol consumption on the surfactant proteins SP-A and SP-D, while these aspects remain unexplored in humans. In view of the high social significance of the problem of alcohol abuse, and also due to the fact that the lungs are one of the "target organs" in excessive alcohol consumption, the study of the effect of alcohol consumption on the levels of surfactant proteins SP-A and SP-D in men and women is undoubtedly topical direction of modern medical science. The aim of the work is to determine the effect of alcohol consumption on the levels of surfactant proteins SP-A and SP-D in men and women in Novosibirsk. Materials and methods. On the basis of the NIITPM-branch of the Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, in 2022, 174 people (87 men and 87 women), aged 45 to 69 years, randomly selected from the residents of Novosibirsk, were examined. Alcohol consumption was assessed using the AUDIT questionnaire. The content of surfactant proteins SP-A and SP-D in blood serum was determined by enzyme immunoassay using standard ELISA kits. Statistical analysis used parametric and non-parametric methods of descriptive statistics, correlation analysis was carried out using the Spearman test, and multivariate analysis using binary logistic regression. ROC-analysis was used to assess the diagnostic significance of variables with the determination of sensitivity and specificity. In all procedures of statistical analysis, the critical significance level of the null statistical hypothesis (p) was taken equal to 0.05. Results. In the general sample of men and women aged 45-69 years, it was determined that the usual single dose of alcohol consumed equal to 7-9 alcohol units is inversely associated with SP-A (r=-0.154, p=0.043). Using ROC analysis, it was found that SP-A is a marker (the area under the ROC curve is 71.2%) of alcohol intake in a typical single dose of alcohol consumed equal to 7-9 alcohol units. With an SP-A level of no more than 921 pg / ml, its sensitivity in relation to the determination of alcohol consumption in a typical single dose of 7-9 alcohol units is 68.7%, and the specificity is 87.5%. Using regression analysis, it was determined that the variable "usual single dose of alcohol consumed equal to 7-9 alcohol units" directly affects the presence of SP-A no more than 921 pg / ml (Exp (B) = 13.0; 95% CI = 1, 5; 111.0, p=0.019) regardless of age and male sex. Conclusion. Thus, it has been established that in men and women of Novosibirsk aged 45-69 years, a high single dose of alcohol consumed equal to 7-9 alcohol units is an independent factor that directly affects the presence of SP-A levels in the blood that do not exceed 921 pg/ml .