bluuebunny/crossref_metadata_2025 huggingface dataset:
Solid Lubrication at High-Temperatures—A Review
Authors: Kumar Rahul , Hussainova Irina , Rahmani Ramin , Antonov Maksim
Published: February 2022

Understanding the complex nature of wear behavior of materials at high-temperature is of fundamental importance for several engineering applications, including metal processing (cutting, forming, forging), internal combustion engines, etc. At high temperatures (up to 1000 °C), the material removal is majorly governed by the changes in surface reactivity and wear mechanisms. The use of lubricants to minimize friction, wear and flash temperature to prevent seizing is a common approach in engine tribology. However, the degradation of conventional liquid-based lubricants at temperatures beyond 300 °C, in addition to its harmful effects on human and environmental health, is deeply concerning. Solid lubricants are a group of compounds exploiting the benefit of wear diminishing mechanisms over a wide range of operating temperatures. The materials incorporated with solid lubricants are herein called ‘self-lubricating’ materials. Moreover, the possibility to omit the use of conventional liquid-based lubricants is perceived. The objective of the present paper is to review the current state-of-the-art in solid-lubricating materials operating under dry wear conditions. By opening with a brief summary of the understanding of solid lubrication at a high temperature, the article initially describes the recent developments in the field. The mechanisms of formation and the nature of tribo-films (or layers) during high-temperature wear are discussed in detail. The trends and ways of further development of the solid-lubricating materials and their future evolutions are identified.
Effect of Cu Content on the Precipitation Behaviors, Mechanical and Corrosion Properties of As-Cast Ti-Cu Alloys
Authors: Wang Zhe , Fu Binguo , Wang Yufeng , Dong Tianshun , Li Jingkun , Li Guolu , Zhao Xuebo , Liu Jinhai , Zhang Guixian
Published: February 2022

Ti-Cu alloys have broad application prospects in the biomedical field due to their excellent properties. The properties of Ti-Cu alloys are strongly dependent on Cu content, microstructures, its Ti2Cu phase and its preparation process. The aim of this work is to investigate the effect of Cu content on the precipitation behaviors, mechanical and corrosion properties of the as-cast Ti-Cu alloys. The microstructures and phase evolution were characterized by SEM and TEM, and the properties were studied by tensile and electrochemical test. The results show that the volume fraction of Ti2Cu phase increases with the increase of Cu content. The Ti2Cu phase presents a variety of microscopic morphologies with different Cu content, such as rod, granular, lath and block shaped. The crystal orientation relationships between the Ti2Cu and α-Ti matrix in Ti-4Cu and Ti-10Cu alloys are (103)Ti2Cu//(0[11¯11)α-Ti, [3¯01]Ti2Cu//[21¯1¯0]α-Ti, and (103)Ti2Cu//(0002)α-Ti, [3¯31]Ti2Cu//[12¯10]α-Ti, respectively. The tensile strength, Vickers hardness and Young’s modulus of the Ti-Cu alloys increase with the increase of Cu content, whereas the elongation decreases. The fracture morphologies of these alloys reveal ductile, ductile-brittle hybrid, and cleavage brittle mode, respectively. The corrosion resistance of the Ti-Cu alloys in SBF solution can be described as: Ti-4Cu alloy > Ti-10Cu alloy > Ti-7Cu alloy. The volume fraction of Ti2Cu phases and the “protective barrier” provided by the fine lath Ti2Cu phases strongly affected the electrochemical performances of the alloys.
Plant-Oil-Based Fibre Composites for Boat Hulls
Authors: Dąbrowska Agnieszka
Published: February 2022

Plant-oil-based fibre composites for boat hulls are an interesting and growing group of materials. Although many problems are to be tackled at different stages of their preparation, the green composites are already successfully commercialised. Within this paper, the most important chemical and physical characteristics of both natural fibres and sustainable resins are provided in the form of a comprehensive review. Finally, the complex issue of interactions filler–matrix is considered.
Shear Performance of RC Beams Reinforced with Fe-Based Shape Memory Alloy Stirrups
Authors: Ji Sang-Won , Yeon Yeong-Mo , Hong Ki-Nam
Published: February 2022

In this study, the shear performance of a reinforced concrete (RC) beam with Fe-based shape memory alloy (Fe-SMA) stirrups was evaluated experimentally and analytically. Five specimens that had a possibility of shear failure under four-point loading were prepared. The major experimental variables were the spacings (300 and 200 mm) between the Fe-SMA stirrups and whether the stirrups were activated or non-activated. The shear strength of the specimen reinforced with the Fe-SMA stirrups at a spacing of 200 mm was 27.1% higher than that of the specimen reinforced at a spacing of 300 mm. The activation of the Fe-SMA stirrups, which produced active confining pressure, increased the shear strength by up to 7.6% and decreased the number of shear cracks compared to the case of the non-activated specimen. Therefore, the use of Fe-SMA stirrups could significantly improve the usability of concrete members by increasing their shear strength and initial stiffness and by controlling crack formation. Furthermore, finite element method (FEM) analysis was conducted using LS-DYNA, a commercial software program, to predict the shear performance of the RC beam reinforced with the Fe-SMA stirrups. The ultimate load and displacement of each specimen were predicted with errors less than 1.4 and 9.4%, respectively. Furthermore, the FEM predicted the change in failure mode and the stiffness improvement due to the activation of the Fe-SMA stirrups. Therefore, the proposed finite element analysis model can effectively predict the behavior of an RC beam reinforced with Fe-SMA stirrups.
Stress Concentration Factors in Excavation Repairs of Surface Defects in Forgings and Castings
Authors: Rebora Alessandro , Torre Giorgio , Vernassa Gianluca
Published: February 2022

This paper provides an analytical formula for the theoretical stress concentration factor in a common type of excavation repair for large forgings and castings. Mechanical components obtained with these processes are often subjected to superficial defects. As the rejection of such pieces is out of question, given the relevant size and costs associated with them, usual industrial practice consists in the removal of the defect and a portion of the surrounding material through milling processes. The authors have selected a reference geometry of the excavation to be left on the mechanical pieces, which can be easily controllable in practice by three operating parameters. Then, the domain of existence of such a repair was investigated on a sequence of discrete points, by means of FEA, obtaining for each, the values of the stress concentration factor Kt. Finally, through polynomial regression, the Kt functions have been accurately approximated by a sixth degree polynomial formulation, which, given a triplet of dimensional geometric parameters, is able to compute the stress concentration factor Kt, with an error that never exceeds 8%.
A New Efficient Approach to Simulate Material Damping in Metals by Modeling Thermoelastic Coupling
Authors: Zacharias Christin , Könke Carsten , Guist Christian
Published: February 2022

The realistic prediction of material damping is crucial in the design and dynamic simulation of many components in mechanical engineering. Material damping in metals occurs mainly due to the thermoelastic effect. This paper presents a new approach for implementing thermoelastic damping into finite element simulations, which provides an alternative to computationally intensive, fully coupled thermoelastic simulations. A significantly better agreement between simulation results and experimental data was achieved, when compared with the empirical damping values found in the literature. The method is based on the calculation of the generated heat within a vibration cycle. The temperature distribution is determined by the mechanical eigenmodes and the energy converted into heat, and thus dissipated, is calculated. This algorithm leads to modal damping coefficients that can then be used in subsequent analyses of dynamically excited oscillations. The results were validated with experimental data obtained from vibration tests. In order to measure material damping only, a test setup excluding friction and environmental influences was developed. Furthermore, comparisons with fully coupled thermoelastic simulations were performed. It was clear that the new approach achieved results comparable to those of a computationally expensive, coupled simulation with regard to the loss factors and frequency response analyses.
Disulfated Ophiuroid Type Steroids from the Far Eastern Starfish Pteraster marsippus and Their Cytotoxic Activity on the Models of 2D and 3D Cultures
Authors: Kicha Alla A. , Kalinovsky Anatoly I. , Malyarenko Timofey V. , Malyarenko Olesya S. , Ermakova Svetlana P. , Popov Roman S. , Stonik Valentin A. , Ivanchina Natalia V.
Published: February 2022

New steroidal 3β,21-disulfates (2–4), steroidal 3β,22-disulfate (5), and the previously known related steroidal 3β,21-disulfate (1) were isolated from the ethanolic extract of the Far Eastern starfish Pteraster marsippus, collected off Urup Island in the Sea of Okhotsk. The structures of these compounds were determined by intensive NMR and HRESIMS techniques as well as by chemical transformations. Steroids 2 and 3 have an oxo-group in the tetracyclic nucleus at position C-7 and differ from each other by the presence of the 5(6)-double bond. The Δ24-22-sulfoxycholestane side chain of the steroid 5 has not been found previously in the starfish or ophiuroid steroids. The cytotoxic activities of 1, 4, 5, and the mixture of 2 and 3 were determined on the models of 2D and 3D cultures of human epithelial kidney cells (HEK293), melanoma cells (SK-MEL-28), small intestine carcinoma cells (HuTu80), and breast carcinoma cells (ZR-75-1). The mixture of 2 and 3 revealed a significant inhibitory effect on the cell viability of human breast carcinoma ZR-75-1 cells, but other tested compounds were less effective.
Calculation of Permeability Coefficients from Solute Equilibration Dynamics: An Assessment of Various Methods
Authors: Cordeiro Margarida M. , Salvador Armindo , Moreno Maria João
Published: February 2022

Predicting the rate at which substances permeate membrane barriers in vivo is crucial for drug development. Permeability coefficients obtained from in vitro studies are valuable for this goal. These are normally determined by following the dynamics of solute equilibration between two membrane-separated compartments. However, the correct calculation of permeability coefficients from such data is not always straightforward. To address these problems, here we develop a kinetic model for solute permeation through lipid membrane barriers that includes the two membrane leaflets as compartments in a four-compartment model. Accounting for solute association with the membrane allows assessing various methods in a wide variety of conditions. The results showed that the often-used expression Papp = β × r/3 is inapplicable to very large or very small vesicles, to moderately or highly lipophilic solutes, or when the development of a significant pH gradient opposes the solute’s flux. We establish useful relationships that overcome these limitations and allow predicting permeability in compartmentalised in vitro or in vivo systems with specific properties. Finally, from the parameters for the interaction of the solute with the membrane barrier, we defined an intrinsic permeability coefficient that facilitates quantitative comparisons between solutes.
Treatment of Hydrothermal-Liquefaction Wastewater with Crossflow UF for Oil and Particle Removal
Authors: Sayegh Ali , Merkert Simon , Zimmermann Joscha , Horn Harald , Saravia Florencia
Published: February 2022

This study aims to evaluate the application of ceramic ultrafiltration membranes in the crossflow mode for the separation of particles and oil in water emulsions (free oil droplets and micelles) from hydrothermal-liquefaction wastewater (HTL-WW) from the hydrothermal liquefaction of municipal sewage sludge. The experiments were carried out using one-channel TiO2 membranes with pore sizes of 30, 10 and 5 nm. The results showed that the highest stable permeability could be achieved with a membrane-pore size of 10 nm, which experienced less fouling, especially through pore blockage, in comparison to the two other pore sizes. Instead of observing an increase in the permeability, the application of a higher feed temperature as well as backwash cycles led to a clear increase in irreversible fouling due to the presence of surfactants in the HTL-WW. Among several physical and chemical cleaning methods, alkaline cleaning at pH 12 proved to be the most efficient in removing fouling and maintaining stable performance on a long-term basis. Ceramic-membrane ultrafiltration can be considered as an adequate first-stage treatment of real HTL wastewater.
Surface Modification of Matrimid® 5218 Polyimide Membrane with Fluorine-Containing Diamines for Efficient Gas Separation
Authors: Lee Tae Hoon , Lee Byung Kwan , Park Jin Sung , Park Jinmo , Kang Jun Hyeok , Yoo Seung Yeon , Park Inho , Kim Yo-Han , Park Ho Bum
Published: February 2022

Polyimide membranes have been widely investigated in gas separation applications due to their high separation abilities, excellent processability, relatively low cost, and stabilities. Unfortunately, it is extremely challenging to simultaneously achieve both improved gas permeability and selectivity due to the trade-off relationship in common polymer membranes. Diamine modification is a simple strategy to tune the separation performance of polyimide membranes, but an excessive loss in permeability is also generally observed. In the present work, we reported the effects of diamine type (i.e., non-fluorinated and fluorinated) on the physicochemical properties and the corresponding separation performance of a modified membrane using a commercial Matrimid® 5218 polyimide. Detailed spectroscopic, thermal, and surface analyses reveal that the bulky fluorine groups are responsible for the balanced chain packing modes in the resulting Matrimid membranes compared to the non-fluorinated diamines. Consequently, the modified Matrimid membranes using fluorinated diamines exhibit both higher gas permeability and selectivity than those of pristine Matrimid, making them especially effective for improving the separation performance towards H2/CH4 and CO2/CH4 pairs. The results indicate that the use of fluorinated modifiers may offer new opportunities to tune the gas transport properties of polyimide membranes.
Comparison of Physicochemical Properties of Two Types of Polyepichlorohydrin-Based Anion Exchange Membranes for Reverse Electrodialysis
Authors: Karakoç Ezgi , Güler Enver
Published: February 2022

The development of the most effective, suitable and economic ion-exchange membranes is crucial for reverse electrodialysis (RED)—the most widely studied process to harvest salinity gradient energy from mixing seawater and river water. RED utilizes two types of membranes as core elements, namely cation exchange membranes (CEM) and anion exchange membranes (AEM). Since the preparation of AEMs is more complex compared to CEMs, the design and development of anion exchange membranes have been the focus in this study. Homogeneous AEMs based on two types of polyepichlorohydrin (PECH) with different chlorine amounts (PECH-H, 37 wt% and PECH-C, 25 wt%) were synthesized, and first-time benchmarking of the membrane properties was conducted. In addition to physicochemical membrane properties, some instrumental analyses such as SEM, FTIR and DSC were investigated to characterize these anion-exchange membranes. Based on the results, although the PECH-H-type membrane had enhanced ion-exchange properties, PECH-C-based anion-exchange membranes exhibited a higher power density of 0.316 W/m2 in a lab-scale RED system. Evidently, there is room for the development of new types of PECH-C-based AEMs with great potential for energy generation in the RED process.
Impact of Cladding Technology on Residual Stresses within the Renovation of High Pressure Die Casting Molds
Authors: Šarga Patrik , Brezinová Janette , Viňáš Ján , Pástor Miroslav , Brezina Jakub
Published: February 2022

Residual stresses significantly affect the overall lifetime of constructions. The main disadvantage of residual stresses is that their effect and magnitude is not evident at first sight and can only be reliably determined using experimental methods. The experiment aimed to find out how much the cladding technology affects the residual stresses within the renovation of high pressure die casting molds. A total of four samples were prepared by different cladding techniques and with varying thicknesses of the result layer. The samples were then annealed and mechanically treated. Finally, the hole drilling method was applied to determine the residual stresses in the samples. The results point to more significant differences in the levels of residual stresses, which were determined in the subsurface layers.
Investigating Metal Solidification with X-ray Imaging
Authors: Feng Shikang , Han Insung , Lui Andrew , Vincent Robin , Ring Gideon , Grant Patrick S. , Liotti Enzo
Published: February 2022

In the last two decades, X-ray imaging techniques have been used increasingly to study metal solidification in real-time as, thanks to advances in X-ray sources (synchrotron and laboratory-based) and detector technology, images can now be obtained with spatio-temporal resolutions sufficient to record key phenomena and extract quantitative information, primarily relating to crystal growth. This paper presents an overview of the research conducted at the University of Oxford over the last 6 years as a partner in the UK’s Future Liquid Metal Engineering (LiME) Manufacturing Hub. The focus is on in situ X-ray radiography to investigate the solidification of Al alloys, including the formation of primary α-Al crystals, and the formation and growth of secondary intermetallic phases. Technologically, the thrust is to understand how to control as-cast phases, structures and element distributions, particularly elements associated with recycling, as a means to facilitate greater recirculation of aluminium alloys. We first present studies on refinement of primary α-Al, including extrinsic grain refinement using inoculation and intrinsic refinement based on dendrite fragmentation. Second, we describe studies on intermetallic phase formation and growth, because intermetallic fraction, morphology and distribution are frequently a limiting factor of alloy mechanical properties and recyclability. Then we present some of the latest progress in studying liquid flow during solidification and associated hot tear formation. Finally, future research directions are described.
Solid-State Fermented Okara with Aspergillus spp. Improves Lipid Metabolism and High-Fat Diet Induced Obesity
Authors: Ichikawa Natsumi , Ng Li Shiuan , Makino Saneyuki , Goh Luo Lin , Lim Yun Jia , Ferdinandus  , Sasaki Hiroyuki , Shibata Shigenobu , Lee Chi-Lik Ken
Published: February 2022

Okara is a major by-product of soymilk and tofu production. Despite retaining abundant nutrients after the process, okara is often under-utilized. In this study, solid-state fermentation (SSF) of okara was carried out using a koji starter (containing both Aspergillus oryzae and Aspergillus sojae) with the intention of releasing its untapped nutrients. Its effects on lipid metabolism in diet-induced obesity (DIO) were observed. The nutritional profile of fermented okara was elucidated using the following parameters: total phenolic content (TPC), pH, protein content, dietary fiber, amino acid content, and free sugar content. In vivo experiments were conducted using high-fat diets supplemented with unfermented okara and fermented okara over three weeks. Supplementation with fermented okara reduced body weight gain, adipose tissue weight, the serum triglyceride profile, and lipid accumulation in the liver, and altered the mRNA expression levels related to lipid metabolism; however, it did not affect pH and short-chain fatty acid (SCFA) production in this study. In conclusion, high-fat diets supplemented using okara fermented with Aspergillus spp. improved the lipid metabolism in mice, due to their high nutritional value, such as TPC, soy protein, and amino acids, and their synergistic effects without altering the gut microbiota.
A Multi-Label Classifier for Predicting the Most Appropriate Instrumental Method for the Analysis of Contaminants of Emerging Concern
Authors: Alygizakis Nikiforos , Konstantakos Vasileios , Bouziotopoulos Grigoris , Kormentzas Evangelos , Slobodnik Jaroslav , Thomaidis Nikolaos S.
Published: February 2022

Liquid chromatography-high resolution mass spectrometry (LC-HRMS) and gas chromatography-high resolution mass spectrometry (GC-HRMS) have revolutionized analytical chemistry among many other disciplines. These advanced instrumentations allow to theoretically capture the whole chemical universe that is contained in samples, giving unimaginable opportunities to the scientific community. Laboratories equipped with these instruments produce a lot of data daily that can be digitally archived. Digital storage of data opens up the opportunity for retrospective suspect screening investigations for the occurrence of chemicals in the stored chromatograms. The first step of this approach involves the prediction of which data is more appropriate to be searched. In this study, we built an optimized multi-label classifier for predicting the most appropriate instrumental method (LC-HRMS or GC-HRMS or both) for the analysis of chemicals in digital specimens. The approach involved the generation of a baseline model based on the knowledge that an expert would use and the generation of an optimized machine learning model. A multi-step feature selection approach, a model selection strategy, and optimization of the classifier’s hyperparameters led to a model with accuracy that outperformed the baseline implementation. The models were used to predict the most appropriate instrumental technique for new substances. The scripts are available at GitHub and the dataset at Zenodo.
Untargeted Metabolomics Identify a Panel of Urinary Biomarkers for the Diagnosis of Urothelial Carcinoma of the Bladder, as Compared to Urolithiasis with or without Urinary Tract Infection in Dogs
Authors: Tsamouri Maria Malvina , Durbin-Johnson Blythe P. , Culp William T. N. , Palm Carrie A. , Parikh Mamta , Kent Michael S. , Ghosh Paramita M.
Published: February 2022

Urothelial carcinoma (UC), the most common urologic cancer in dogs, is often diagnosed late because the clinical signs are shared by other non-malignant lower urinary tract disorders (LUTD). The urine-based BRAFV595E test for UC is highly effective only in certain breeds; hence additional non-invasive biomarkers of UC are needed. Here, urine from dogs with UC (n = 27), urolithiasis (n = 8), or urolithiasis with urinary tract infection (UTI) (n = 8) were subjected to untargeted metabolomics analyses, using GC-TOF-MS for primary metabolites, QTOF-MS for complex lipids, and HILIC-QTOF MS for secondary and charged metabolites. After adjusting for age and sex, we identified 1123 known metabolites that were differentially expressed between UC and LUTD. Twenty-seven metabolites were significant (1.5 ≤ log2FC ≤ −1.5, adjusted p-value < 0.05); however, 10 of these could be attributed to treatment-related changes. Of the remaining 17, 6 (hippuric acid, N-Acetylphenylalanine, sarcosine, octanoylcarnitine, N-alpha-methylhistamine, glycerol-3-galactoside) discriminated between UC and LUTD (area under the ROC curve > 0.85). Of the 6 metabolites, only hippuric acid and N-alpha-methylhistamine were discriminatory in both male (n = 20) and female (n = 23) dogs, while sarcosine was an effective discriminator in several breeds, but only in females. Further investigation of these metabolites is warranted for potential use as non-invasive diagnostic biomarkers of dogs with UC that present with LUTD-related clinical signs.
Hyaluronic Acid–Zein Core-Shell Nanoparticles Improve the Anticancer Effect of Curcumin Alone or in Combination with Oxaliplatin against Colorectal Cancer via CD44-Mediated Cellular Uptake
Authors: Liu Lu , Yang Shufang , Chen Feng , Cheng Ka-Wing
Published: February 2022

Curcumin (CUR) has been reported to enhance the chemotherapeutic efficacy of oxaliplatin (OXA) in colorectal cancer (CRC) and inhibit OXA-induced side effects. However, shortcomings, including poor solubility and sensitivity to metabolic transformation, have greatly undermined its value in clinical applications. In this study, the potential of CUR-encapsulated hyaluronic acid (HA)–zein composite nanoparticles (HZ-CUR) as an oral adjuvant for OXA-based chemotherapy was assessed in representative CRC models in mice. Cell viability and colony formation assays in three human CRC cell lines showed that HZ-CUR had a stronger anti-CRC effect than free CUR when given alone and a stronger synergistic effect when combined with OXA, especially in HCT116 and HT29 cell lines. Western blotting, cellular uptake, and RNA interference assays revealed that OXA-induced upregulation of CD44 likely contributed to enhanced cellular uptake of HZ-CUR and thus the enhanced anticancer effect. The significantly improved anti-CRC effects and potential underlying mechanism of HZ-CUR alone and in combination with OXA were further validated in a subcutaneous xenograft and an in situ CRC model in mice. These findings support that HZ-CUR may be an effective oral adjuvant for OXA-based CRC chemotherapy that would not only improve its efficacy but also help reduce the associated side effects.
Investigation of Sulfonated Graphene Oxide as the Base Material for Novel Proton Exchange Membranes
Authors: Basso Peressut Andrea , Di Virgilio Matteo , Bombino Antonella , Latorrata Saverio , Muurinen Esa , Keiski Riitta L. , Dotelli Giovanni
Published: February 2022

This work deals with the development of graphene oxide (GO)-based self-assembling membranes as possible innovative proton conductors to be used in polymer electrolyte membrane fuel cells (PEMFCs). Nowadays, the most adopted electrolyte is Chemours’ Nafion; however, it reveals significant deficiencies such as strong dehydration at high temperature and low humidity, which drastically reduces its proton conductivity. The presence of oxygenated moieties in the GO framework makes it suitable for functionalization, which is required to enhance the promising, but insufficient, proton-carrying features of GO. In this study, sulfonic acid groups (–SO3H) that should favor proton transport were introduced in the membrane structure via a reaction between GO and concentrated sulfuric acid. Six acid-to-GO molar ratios were adopted in the synthesis procedure, giving rise to final products with different sulfonation degrees. All the prepared samples were characterized by means of TGA, ATR-FTIR and Raman spectroscopy, temperature-dependent XRD, SEM and EDX, which pointed out morphological and microstructural changes resulting from the functionalization stage, confirming its effectiveness. Regarding functional features, electrochemical impedance spectroscopy (EIS) as well as measurements of ion exchange capacity (IEC) were carried out to describe the behavior of the various samples, with pristine GO and commercial Nafion® 212 used as reference. EIS tests were performed at five different temperatures (20, 40, 60, 80 and 100 °C) under high (95%) and medium (42%) relative humidity conditions. Compared to both GO and Nafion® 212, the sulfonated specimens demonstrate an increase in the number of ion-carrying groups, as proved by both IEC and EIS tests, which reveal the enhanced proton conductivity of these novel membranes. Specifically, an acid-to-GO molar ratio of 10 produces a six-fold improvement of IEC (4.23 meq g−1) with respect to pure GO (0.76 meq g−1), while a maximum eight-fold improvement (5.72 meq g−1) is achieved in SGO-15.
Archaeal and Bacterial Content in a Two-Stage Anaerobic System for Efficient Energy Production from Agricultural Wastes
Authors: Kabaivanova Lyudmila , Hubenov Venelin , Dimitrova Lyudmila , Simeonov Ivan , Wang Haoping , Petrova Penka
Published: February 2022

Anaerobic digestion (AD) is a microbially-driven process enabling energy production. Microorganisms are the core of anaerobic digesters and play an important role in the succession of hydrolysis, acidogenesis, acetogenesis, and methanogenesis processes. The diversity of participating microbial communities can provide new information on digester performance for biomass valorization and biofuel production. In this study anaerobic systems were used, operating under mesophilic conditions that realized biodegradation processes of waste wheat straw pretreated with NaOH—a renewable source for hydrogen and methane production. These processes could be managed and optimized for hydrogen and methane separately but combining them in a two-stage system can lead to higher yields and a positive energy balance. The aim of the study was to depict a process of biohydrogen production from lignocellulosic waste followed by a second one leading to the production of biomethane. Archaeal and bacterial consortia in a two-stage system operating with wheat straw were identified for the first time and the role of the most important representatives was elucidated. The mixed cultures were identified by the molecular-biological methods of metagenomics. The results showed that biohydrogen generation is most probably due to the presence of Proteiniphilum saccharofermentans, which was 28.2% to 45.4% of the microbial community in the first and the second bioreactor, respectively. Archaeal representatives belonging to Methanobacterium formicicum (0.71% of the community), Methanosarcina spelaei (0.03%), Methanothrix soehngenii (0.012%), and Methanobacterium beijingense (0.01%) were proven in the methane-generating reactor. The correlation between substrate degradation and biogas accumulation was calculated, together with the profile of fatty acids as intermediates produced during the processes. The hydrogen concentration in the biogas reached 14.43%, and the Methane concentration was 69%. Calculations of the energy yield during the two-stage process showed 1195.89 kWh·t−1 compared to a 361.62 kWh·t−1 cumulative yield of energy carrier for a one-stage process.
Recent Advancements in Mitochondria-Targeted Nanoparticle Drug Delivery for Cancer Therapy
Authors: Xu Jiangsheng , Shamul James , Kwizera Elyahb , He Xiaoming
Published: February 2022

Mitochondria are critical subcellular organelles that produce most of the adenosine triphosphate (ATP) as the energy source for most eukaryotic cells. Moreover, recent findings show that mitochondria are not only the “powerhouse” inside cells, but also excellent targets for inducing cell death via apoptosis that is mitochondria-centered. For several decades, cancer nanotherapeutics have been designed to specifically target mitochondria with several targeting moieties, and cause mitochondrial dysfunction via photodynamic, photothermal, or/and chemo therapies. These strategies have been shown to augment the killing of cancer cells in a tumor while reducing damage to its surrounding healthy tissues. Furthermore, mitochondria-targeting nanotechnologies have been demonstrated to be highly efficacious compared to non-mitochondria-targeting platforms both in vitro and in vivo for cancer therapies. Moreover, mitochondria-targeting nanotechnologies have been intelligently designed and tailored to the hypoxic and slightly acidic tumor microenvironment for improved cancer therapies. Collectively, mitochondria-targeting may be a promising strategy for the engineering of nanoparticles for drug delivery to combat cancer.
Efficient Saturable Absorber Based on Ferromagnetic Insulator Cr2Ge2Te6 in Er-Doped Mode-Locked Fiber Laser
Authors: Sun Ruyi , Guo Linguang , Shang Xinxin , Zhang Huanian , Yue Qingyang
Published: February 2022

A ferromagnetic insulator Cr2Ge2Te6 as a saturable absorber in an Er-doped fiber laser (EDFL) was demonstrated. In this work, a CGT-PVA composite film was successfully fabricated using the liquid-phase exfoliation method and employed in an EDFL. The modulation depth and saturation intensity of the SA are 4.26% and 89.40 MW/cm2, respectively. Stable pulses with a minimum pulse width of 978.5 fs when the repetition rate was 3.25 MHz were recorded experimentally. Furthermore, stable solitons still need to be obtained when the pulse energy in the cavity is as high as 11.6 nJ. The results fully suggest that CGT has outstanding nonlinear absorption properties, which may have broad potential applications in ultrafast photons.
A Comparison of Dietary Patterns and Factors Influencing Food Choice among Ethnic Groups Living in One Locality: A Systematic Review
Authors: Bennett Grace , Bardon Laura A. , Gibney Eileen R.
Published: February 2022

Globally, the number of minority ethnic groups in high-income countries is increasing. However, despite this demographic change, most national food consumption surveys are not representative of ethnically diverse populations. In consequence, many ethnic minorities’ dietary intakes are underreported, meaning that accurate analysis of food intake and nutrient status among these groups is not possible. This systematic review aims to address these gaps and understand differences in dietary intakes and influencers of dietary habits of ethnic groups worldwide. A systematic search was conducted through three databases (Pubmed, Web of Science and Scopus) and manual searches, generating n = 56,647 results. A final search of these databases was completed on 13 September 2021, resulting in a total of 49 studies being included in this review. Overall, food group intakes—particularly fruit, vegetable and fish intake—and diet quality scores were seen to differ between ethnicities. Overall Black/African American groups were reported to be among the poorest consumers of fruit and vegetables, whilst Asian groups achieved high diet quality scores due to higher fish intakes and lower fat intakes compared to other groups. Limited data investigated how nutrient intakes, dietary and meal patterns compared between groups, meaning that not all aspects of dietary intake could be compared. Socioeconomic status and food availability appeared to be associated with food choice of ethnic groups, however, confounding factors should be considered more closely. Future work should focus on comparing nutrient intakes and meal patterns between ethnicities and investigate potential targeted interventions which may support adherence to food-based dietary guidelines by all ethnic groups.
Development and Validation of Cutoff Value for Reduced Muscle Mass for GLIM Criteria in Patients with Gastrointestinal and Hepatobiliary–Pancreatic Cancers
Authors: Takimoto Mami , Yasui-Yamada Sonoko , Nasu Nanami , Kagiya Natsumi , Aotani Nozomi , Kurokawa Yumiko , Tani-Suzuki Yoshiko , Kashihara Hideya , Saito Yu , Nishi Masaaki , Shimada Mitsuo , Hamada Yasuhiro
Published: February 2022

The Global Leadership Initiative on Malnutrition (GLIM) criteria recommends using race- and sex-adjusted cutoff values for reduced muscle mass (RMM), but the only cutoff values available for Asians are the skeletal muscle mass index (SMI) established by the Asian Working Group for Sarcopenia (AWGS). This retrospective study aimed to develop and validate cutoff values for the fat-free mass index (FFMI) and arm circumference (AC) of Asians, and to investigate the association between GLIM malnutrition and prognosis. A total of 660 patients with primary gastrointestinal (GI) and hepatobiliary–pancreatic (HBP) cancers who underwent their first resection surgery were recruited and randomly divided into development and validation groups. The FFMI and AC cutoff values were calculated by receiver operating characteristic curve analysis for the AWGS SMI as the gold standard. The cutoff values for each RMM were used to diagnose malnutrition on the basis of GLIM criteria, and the survival rates were compared. The optimal FFMI cutoff values for RMM were 17 kg/m2 for men and 15 kg/m2 for women, and for AC were 27 cm for men and 25 cm for women. In the validation group, the accuracy of the FFMI and AC cutoff values to discriminate RMM were 85.2% and 68.8%, respectively. Using any of the three measures of RMM, overall survival rates were significantly lower in the GLIM malnutrition group. In conclusion, the cutoff values for the FFMI and AC in this study could discriminate RMM, and GLIM malnutrition using these cutoff values was associated with decreased survival.
Selenium Status in Patients with Chronic Liver Disease: A Systematic Review and Meta-Analysis
Authors: Lin Yaduan , He Fanchen , Lian Shaoyan , Xie Binbin , Liu Ting , He Jiang , Liu Chaoqun
Published: February 2022

Background: The potential role of selenium in preventing chronic liver diseases remains controversial. This meta-analysis aimed to summarize the available evidence from observational studies and intervention trials that had evaluated the associations between body selenium status and chronic liver diseases. Methods: We comprehensively searched MEDLINE, Embase, Web of Science, and Cochrane Library from inception to April 2021. The study protocol was registered at PROSPERO (CRD42020210144). Relative risks (RR) for the highest versus the lowest level of selenium and standard mean differences (SMD) with 95% confidence intervals (CI) were pooled using random-effects models. Heterogeneity and publication bias were evaluated using the I2 statistic and Egger’s regression test, respectively. Results: There were 50 studies with 9875 cases and 12975 population controls in the final analysis. Patients with hepatitis (SMD = −1.78, 95% CI: −2.22 to −1.34), liver cirrhosis (SMD = −2.06, 95% CI: −2.48 to −1.63), and liver cancer (SMD = −2.71, 95% CI: −3.31 to −2.11) had significantly lower selenium levels than controls, whereas there was no significant difference in patients with fatty liver diseases (SMD = 1.06, 95% CI: −1.78 to 3.89). Moreover, the meta-analysis showed that a higher selenium level was significantly associated with a 41% decrease in the incidence of significant advanced chronic liver diseases (RR = 0.59, 95% CI: 0.49 to 0.72). Conclusion: Our meta-analysis suggested that both body selenium status and selenium intake were negatively associated with hepatitis, cirrhosis, and liver cancer. However, the associations for fatty liver diseases were conflicting and need to be established in prospective trials.
Mechanism of Astragalus membranaceus Alleviating Acquired Hyperlipidemia Induced by High-Fat Diet through Regulating Lipid Metabolism
Authors: Wang Ling , Zheng Wenya , Yang Jinxin , Ali Anwar , Qin Hong
Published: February 2022

Astragalus membranaceus (AM) is a food and medicinal homologous plant. The current research is aimed to investigate the beneficial effects and mechanisms of AM in treating acquired hyperlipidemia. The network pharmacology and bioinformatics analysis results showed 481 AM-related targets and 474 acquired hyperlipidemia-associated targets, and 101 candidate targets were obtained through the intersection, mainly enriched in endocrine resistance, AGE-RAGE in diabetic complications and p53 signaling pathways. Quercetin, kaempferol, calycosin, formononetin and isorhamnetin were determined as the candidate active components of AM in the treatment of acquired hyperlipidemia. Moreover, key targets of AM, namely, AKT serine/threonine kinase 1 (AKT1), vascular endothelial growth factor A (VEGFA), cyclin D1 (CCND1) and estrogen receptor 1 (ESR1), were screened out, which were closely related to adipogenesis, fatty acid metabolism and bile acid metabolism. The subsequent animal experiments showed that AM extract treatment improved the lipid profiles of the high-fat diet (HFD)-fed mice by reducing lipogenesis and increasing lipolysis and lipid β-oxidation, which were associated with the downregulating of AKT1 and CCND1, and the upregulating of VEGFA and ESR1 in liver and adipose tissue. Overall, AM alleviated acquired hyperlipidemia through regulating lipid metabolism, and AKT1, VEGFA, CCND1 and ESR1 might be the key targets.
Journey on VX-809-Based Hybrid Derivatives towards Drug-like F508del-CFTR Correctors: From Molecular Modeling to Chemical Synthesis and Biological Assays
Authors: Parodi Alice , Righetti Giada , Pesce Emanuela , Salis Annalisa , Tomati Valeria , Pastorino Cristina , Tasso Bruno , Benvenuti Mirko , Damonte Gianluca , Pedemonte Nicoletta , Cichero Elena , Millo Enrico
Published: February 2022

Cystic fibrosis (CF) is a genetic disease affecting the lungs and pancreas and causing progressive damage. CF is caused by mutations abolishing the function of CFTR, a protein whose role is chloride’s mobilization in the epithelial cells of various organs. Recently a therapy focused on small molecules has been chosen as a main approach to contrast CF, designing and synthesizing compounds acting as misfolding (correctors) or defective channel gating (potentiators). Multi-drug therapies have been tested with different combinations of the two series of compounds. Previously, we designed and characterized two series of correctors, namely, hybrids, which were conceived including the aminoarylthiazole (AAT) core, merged with the benzodioxole carboxamide moiety featured by VX-809. In this paper, we herein proceeded with molecular modeling studies guiding the design of a new third series of hybrids, featuring structural variations at the thiazole moiety and modifications on position 4. These derivatives were tested in different assays including a YFP functional assay on models F508del-CFTR CFBE41o-cells, alone and in combination with VX-445, and by using electrophysiological techniques on human primary bronchial epithelia to demonstrate their F508del-CFTR corrector ability. This study is aimed (i) at identifying three molecules (9b, 9g, and 9j), useful as novel CFTR correctors with a good efficacy in rescuing the defect of F508del-CFTR; and (ii) at providing useful information to complete the structure–activity study within all the three series of hybrids as possible CFTR correctors, supporting the development of pharmacophore modelling studies, taking into account all the three series of hybrids. Finally, in silico evaluation of the hybrids pharmacokinetic (PK) properties contributed to highlight hybrid developability as drug-like correctors.
Potential of Industrial Hemp for Phytoremediation of Heavy Metals
Authors: Placido Dante F. , Lee Charles C.
Published: February 2022

The accumulation of anthropogenic heavy metals in soil is a major form of pollution. Such potentially toxic elements are nonbiodegradable and persist for many years as threats to human and environmental health. Traditional forms of remediation are costly and potentially damaging to the land. An alternative strategy is phytoremediation, where plants are used to capture metals from the environment. Industrial hemp (Cannabis sativa) is a promising candidate for phytoremediation. Hemp has deep roots and is tolerant to the accumulation of different metals. In addition, the crop biomass has many potential commercial uses after harvesting is completed. Furthermore, the recent availability of an annotated genome sequence provides a powerful tool for the bioengineering of C. sativa for better phytoremediation.
Phytoremediation of Soils Contaminated with Heavy Metals from Gold Mining Activities Using Clidemia sericea D. Don
Authors: Durante-Yánez Elvia Valeria , Martínez-Macea María Alejandra , Enamorado-Montes Germán , Combatt Caballero Enrique , Marrugo-Negrete José
Published: February 2022

Soils contaminated by potentially toxic elements (PTEs) as a result of anthropogenic activities such as mining are a problem due to the adverse effects on human and environmental health, making it necessary to seek sustainable strategies to remediate contaminated areas. The objective of this study was to evaluate the species Clidemia sericea D. Don for the phytoremediation of soils contaminated with PTEs (Hg, Pb, and Cd) from gold mining activities. The study was conducted for three months, with soils from a gold mining area in northern Colombia, and seeds of C. sericea, under a completely randomized experimental design with one factor (concentration of PTEs in soil) and four levels (control (T0), low (T1), medium (T2), and high (T3)), each treatment in triplicate, for a total of twelve experimental units. Phytotoxic effects on plants, bioconcentration (BCF), and translocation (TF) factors were determined. The results obtained for the tissues differed in order of metal accumulation, with the root showing the highest concentration of metals. The highest values of bioconcentration (BCF > 1) were presented for Hg at T3 and Cd in the four treatments; and of translocation (TF > 1) for Hg and Pb at T0 and T1; however, for Pb, the TF indicates that it is transferable, but it is not considered for phytoextraction. Thus, C. sericea demonstrated its potential as a phytostabilizer of Hg and Cd in mining soils, strengthening as a wild species with results of resistance to the stress of the PTEs evaluated, presenting similar behavior and little phytotoxic affectation on the growth and development of each of the plants in the different treatments.
Exudates of Picea abies, Pinus nigra, and Larix decidua: Chromatographic Comparison and Pro-Migratory Effects on Keratinocytes In Vitro
Authors: Goels Thomas , Eichenauer Elisabeth , Tahir Ammar , Prochaska Paul , Hoeller Franziska , Heiß Elke H. , Glasl Sabine
Published: February 2022

Balms and resins of Picea abies, Larix decidua, and Pinus nigra are traditionally used to treat wounds. Three chromatographic techniques differing in separation capacity and technical demands were employed to distinguish among these plant exudates. A TLC method was established for fingerprint comparison, providing a quick overview of a large number of samples at low cost. HPLC-DAD (RP18) and UHPSFC-DAD (Torus 2-Picolylamin), hyphenated to ESI-MS, represented orthogonal chromatographic systems with high separation performance. The developed methods allow for the separation and detection of major and minor constituents belonging to different compound classes (phenyl carboxylic acids, lignans, diterpene resin acids). The qualitative compositions of the diterpene resin acids, the main compounds in the exudates, were comparable in all three genera. Differences were detected in the distribution of hydroxylated diterpene resin acids, pinoresinol, and hydroxycinnamic acids. The three tested chromatographic systems with varying demands on lab equipment offer appropriate tools for the quality assessment of Picea abies, Larix decidua, and Pinus nigra. The extracts were furthermore tested at three different concentrations (10 µg/mL, 3 µg/mL, and 1 µg/mL) for boosted re-epithelialization, a crucial step in the wound-healing process, in an in vitro HaCaT keratinocyte-based scratch assay. Lysophosphatidic acid (LPA, 10 µM) and extracts of several medicinal plants well known for their wound-healing properties (birch, marigold, St. John’s wort, manuka honey) were used as positive controls. Picea abies and Pinus nigra showed concentration dependency; significant activity was measured for Larix decidua at 3 µg/mL.