bluuebunny/crossref_metadata_2025 huggingface dataset:
Chemical composition, antioxidant properties, and antifungal activity of wild Origanum elongatum extracts against Phytophthora infestans
Authors: Hari Amal , Echchgadda Ghizlane , Darkaoui Fatima-Azzahra , Taarji Noamane , Sahri Nihad , Sobeh Mansour , Ezrari Said , Laasli Salah-Eddine , Benjelloun Meryem , Lahlali Rachid
Published: January 2024

Introduction

Phytophthora infestans, the causative agent of late blight disease, has gained notoriety for its destructive potential, leading to substantial losses in potato yields. Although conventional systemic fungicides have been shown to be effective in controlling plant pathogens, growing environmental concerns have prompted the need for more integrated disease management approaches. Hence, in this study, the effectiveness of wild Origanum elongatum extracts as biopesticides was explored in controlling P. infestans and potentially mitigating its devastating impact in planta.

Methods

The aerial parts of O. elongatum were subjected to sequential extraction using water, hexane, chloroform, and methanol. The obtained extracts were tested in vitro through the poisoned food procedure for their capacity to obstruct P. infestans growth and to defeat potato blight severity in vivo. The phyto-contents (total phenolic content (TPC) and total flavonoid content (TFC)), as well as the antioxidant activities, were spectrophotometrically determined in all extracts, and the phytoconstituents of the most active extract (methanolic extract) were profiled via high-performance liquid chromatography–photodiode array–tandem mass spectrometry (HPLC–PDA–MS/MS).

Results

In vitro, the complete inhibition rate of the P. infestans was obtained using the methanolic extract at 5 mg/mL, followed by the hexane and chloroform extracts at 10 mg/mL. Interestingly, complete inhibition of the pathogen was achieved upon the application of the aqueous extract at 10 mg/mL. In vivo, the aqueous extract at 25 mg/mL reduced the P. infestans severity rate to 27.25%, while the methanolic extract at 20 mg/mL led to the lowest severity rate. Moreover, the hexane and chloroform extracts impaired the pathogen severity rate to 50% and 41% using 20 mg/mL, respectively. The TPC and TFC in the extracts were variable with high concentrations detected in the methanolic extract with 485.42 mg GAE/g and 58.24 mg QE/g, respectively. In addition, the methanolic extract showed the highest antioxidant activities, while the chloroform extract exhibited the lowest activity. Liquid chromatography (LC)–MS/MS analysis of the methanol extract revealed 56 components from diverse classes. These included organic acids, phenolic acids, flavonoids, tannins, and coumarins.

Conclusion

These findings suggest that O. elongatum could be investigated as a potential source of antifungal compounds targeting different phytopathogens.
Comprehensive phenomic and genomic studies of the species, Pectobacterium cacticida and proposal for reclassification as Alcorniella cacticida comb. nov
Authors: Jonca Joanna , Pirhonen Minna , Waleron Michal Mateusz , Gawor Jan , Mrozik Agnieszka , Smoktunowicz Magdalena , Waleron Krzysztof , Waleron Malgorzata
Published: January 2024

Introduction

Pectobacterium cacticida was identified as the causative agent of soft rot disease in cacti. Due to a high potential of spread in the face of global warming, the species poses a significant threat to horticultural and crop industry. The aim of this study was to revise the genomic, physiology and virulence characteristics of P. cacticida and update its phylogenetic position within the Pectobacterium genus.

Methods

Whole genome sequences of five P. cacticida strains were obtained and subjected to comprehensive genomic and phylogenomic data analyses. We assessed the presence of virulence determinants and genes associated with host and environmental adaptation. Lipidomic analysis, as well as biochemical and phenotypic assays were performed to correlate genomic findings.

Results

Phylogenomic analysis revealed that P. cacticida forms a distinct lineage within the Pectobacterium genus. Genomic evaluation uncovered 516 unique proteins, most of which were involved in cellular metabolism. They included genes of carbohydrate metabolism and transport and ABC transporters. The main differing characteristics from other Pectobacterium species were the lack of a myo-inositol degradation pathway and the presence of the malonate decarboxylase gene. All tested strains were pathogenic towards Opuntia spp., chicory, Chinese cabbage, and potato, but exhibited only mild pathogenicity towards carrot.

Discussion

This study sheds light into the genomic characteristics of P. cacticida and highlights the pathogenic potential of the species. Unique genes found in P. cacticida genomes possibly enhance the species’ survival and virulence. Based on phylogenomic analyses, we propose the reclassification of P. cacticida to a new genus, Alcorniella comb. nov.
Drought stress reduces the photosynthetic source of subtending leaves and the transit sink function of podshells, leading to reduced seed weight in soybean plants
Authors: Du Xiangbei , Zhang Xinyue , Chen Xiaofei , Jin Wenjun , Huang Zhiping , Kong Lingcong
Published: January 2024

Drought stress is the key factor limiting soybean yield potential. Soybean seed formation involves a coordinated “subtending leaf-podshell-seed” process, but little is known about the assimilation and transport of photoassimilates in subtending leaves, podshells and seeds or their relationships with soybean seed formation under drought stress. To address these research gaps, two-year experiments with two soybean cultivars, Wandou 37 (drought tolerant) and Zhonghuang 13 (drought sensitive), were conducted under three soil water content (SWC) conditions in 2020 and 2021 based on the responses of their yield to drought. We analyzed the photosynthetic assimilation and translocation of photoassimilates in subtending leaves, podshells and seeds by stable isotope labeling. Compared with those under 75% SWC, 60% SWC and 45% SWC significantly decreased the Wandou 37 seed weight by 19.4% and 37.5%, respectively, and that of Zhonghuang 13 by 26.9% and 48.6%, respectively. Compared with those under 75% SWC, drought stress decreased the net photosynthetic rate and the activities of sucrose phosphate synthase (SPS) and sucrose synthase (SuSy), which in turn decreased the photosynthetic capacity of the subtending leaves. The podshells ensure the input of photoassimilates by increasing the SuSy activity, but the weakened source–sink relationship between podshells and seeds under drought stress leads to a decrease in the translocation of assimilates from podshells to seeds. The lack of assimilates under drought stress is an important factor restricting the development of soybean seeds. We conclude that the decrease in seed weight was caused by the decrease in the photosynthetic capacity of the subtending leaves and the decrease in the overall availability of photoassimilates; moreover, by a decrease in the translocation of assimilates from podshells to seeds.
Unraveling the drought-responsive transcriptomes in nodules of two common bean genotypes during biological nitrogen fixation
Authors: da Silva Helder Anderson Pinto , Caetano Vanessa Santana , Pessôa Daniella Duarte Villarinho , Pacheco Rafael Sanches , Meneses Carlos Henrique S. G. , Simões-Araújo Jean Luiz
Published: January 2024

Common bean (Phaseolus vulgaris) can efficiently fix atmospheric nitrogen when associated with Rhizobia. However, drought stress impairs plant metabolic processes, especially the biological nitrogen fixation (BNF). Here, we assessed transcriptional responses in nodules of two common bean genotypes to drought stress under BNF reliance. The RNA-Seq analysis yielded a total of 81,489,262 and 72,497,478 high quality reads for Negro Argel and BAT 477 genotypes, respectively. The reads were mapped to the Phaseolus vulgaris reference genome and expression analysis identified 145 and 1451 differentially expressed genes (DEGs) for Negro Argel and BAT 477 genotypes, respectively. Although BAT 477 had more DEGs, both genotypes shared certain drought-responsive genes, including an up-regulated heat shock protein (HSP) and a down-regulated peroxidase, indicating shared pathways activated during drought in nodule tissue. Functional analysis using MapMan software highlighted the up-regulation of genes involved in abiotic stress responses, such as HSPs and specific transcription factors (TFs), in both genotypes. There was a significant down-regulation in metabolic pathways related to antioxidant protection, hormone signaling, metabolism, and transcriptional regulation. To validate these findings, we conducted RT-qPCR experiments for ten DEGs in nodules from both genotypes, for which the expression profile was confirmed, thus reinforcing their functional relevance in the nodule responses to drought stress during BNF. BAT 477 genotype exhibited more pronounced response to drought, characterized by a high number of DEGs. The strong down-regulation of DEGs leads to transcriptional disturbances in several pathways related to stress acclimation such as hormone and antioxidant metabolism. Additionally, we identified several genes that are known to play key roles in enhancing drought tolerance, such as HSPs and crucial TFs. Our results provide new insights into the transcriptional responses in root-nodules, an underexplored tissue of plants mainly under drought conditions. This research paves the way for potential improvements in plant-bacteria interactions, contributing to common bean adaptations in the face of challenging environmental conditions.
The impact of misinformation presented during jury deliberation on juror memory and decision-making
Authors: Cullen Hayley J. , Dilevski Natali , Nitschke Faye T. , Ribeiro Gianni , Brind Shobanah , Woolley Nikita
Published: January 2024

When deliberating, jurors may introduce misinformation that may influence other jurors’ memory and decision-making. In two studies, we explored the impact of misinformation exposure during jury deliberation. Participants in both studies read a transcript of an alleged sexual assault. In Study 1 (N = 275), participants encountered either consistent pro-prosecution misinformation, consistent pro-defense misinformation, or contradictory misinformation (pro-prosecution and pro-defense). In Study 2 (N = 339), prior to encountering either pro-prosecution or pro-defense misinformation while reading a jury deliberation transcript, participants either received or did not receive a judicial instruction about misinformation exposure during deliberation. Participants in both studies completed legal decision-making variables (e.g., defendant guilt rating) before and after deliberation, and their memory was assessed for misinformation acceptance via recall and source memory tasks. In Study 1, misinformation type did not influence legal decision-making, but pro-prosecution misinformation was more likely to be misattributed as trial evidence than pro-defense or contradictory misinformation. In Study 2, pro-defense misinformation was more likely to be misattributed to the trial than pro-prosecution misinformation, and rape myths moderated this. Furthermore, exposure to pro-defense misinformation skewed legal decision-making towards the defense’s case. However, the judicial instruction about misinformation exposure did not influence memory or decision-making. Together, these findings suggest that misinformation in jury deliberations may distort memory for trial evidence and bias decision-making, highlighting the need to develop effective safeguards for reducing the impact of misinformation in trial contexts.
How talent management execution impacts career experiences: exploring the organization-individual intersect
Authors: van der Merwe Marna , Nel Petrus , Hoole Crystal
Published: January 2024

The aim of the research was to investigate the ways in which talent management execution impacts the individual career experiences of talent, specifically exploring how talent management execution and career enablement can be closer aligned to unlock value for both the organization and the individual. A qualitative research design was employed to explore the research question. During the first phase of the research data was collected from 13 talent management professionals using purposeful sampling and semi-structured interviews. During the second phase of the research the Delphi method was used to validate the findings (n = 8). Four broad themes were identified as the most prominent ways in which talent management execution impacts the career experiences of individuals. The first is the philosophical underpinning that the organization holds in how talent is defined as well as the exclusivity or inclusivity of their approach. The second is the identification criteria used to identify talent within the organization. Thirdly, the extent to which talent management practices are aligned with career enablement practices and the congruence between these. Lastly, the role of the psychological contract and employee experience in managing mutual expectations. The resultant tension must be proactively managed by (1) clearly articulating the talent philosophy and making this visible through talent management execution, (2) ensuring alignment between talent management practices and the career enablement available to talent, and (3) delivering on expectations through the lived experiences of talent. The study contributes to the existing body of knowledge on talent management and careers, with a specific focus on the intersection between these and defining ways to align these practices to create congruent and authentic career experiences for individuals.
ESport programs in high school: what’s at play?
Authors: Lemay Antoine , Dufour Magali , Goyette Mathieu , Berbiche Djamal
Published: January 2024

Background

A growing number of high schools in Canada offer eSports (ES) in their facilities, which raises concerns regarding this activity’s potential health risks for adolescents.

Methods

The aim of this study is to describe the characteristics of 67 adolescent ES players (ESp) and to compare them to 109 recreational gamers in their high school (nESp). The two groups were compared on (1) sociodemographic and academic characteristics; (2) online and offline activities; (3) psychological characteristics.

Results

Results show that ESp spend more time on online activities and report a higher proportion of problematic gaming compared to the nESp group. ESp report more often that gaming has positive consequences on their physical health and report more often negative consequences on their education compared to the nESp group.

Conclusion

These results underscore the importance of screening gaming problems among adolescent ES players. Targeted prevention should be carried out with these teenagers and in order to be adapted, prevention efforts should consider both, the positive and negative consequences that ESp experience from gaming.
Case report: A case of anti-recoverin antibody-positive encephalitis exhibiting Cotard and Capgras delusions that was successfully treated with electroconvulsive therapy
Authors: Akahane Takaki , Takahashi Naomi , Kobayashi Ryota , Nomura Konoka , Akiho Masakazu , Shikama Yukihiro , Noto Keisuke , Suzuki Akihito
Published: January 2024

Recoverin is a neuron-specific calcium-binding protein that is mainly located in the retina and pineal gland. Few reports have described patients with anti-recoverin antibody-positive encephalitis, and no cases of psychosis associated with this encephalitis have been reported. We report a patient with anti-recoverin antibody-positive encephalitis with Cotard and Capgras delusions who was successfully treated with electroconvulsive therapy (ECT). The patient was a 25-year-old woman. She exhibited disorientation, executive function deficits, tremors in the upper limbs, generalized athetoid-like involuntary movements, hallucinations, incontinence, and fever, which led to her admission to our hospital. Upon admission, she complained of Cotard delusions. Various diagnostic tests, including cerebrospinal fluid analysis, antibody screening, and brain imaging, were unremarkable, except for positivity for serum anti-recoverin antibodies, non-specific general slowing on electroencephalography and decreased regional cerebral blood flow (rCBF) in the frontal and occipital lobes, and increased rCBF in the basal ganglia and pons on single-photon emission computed tomography. She was eventually diagnosed with encephalitis positive for anti-recoverin antibodies and treated with immunoglobulins and steroids. Her neurological symptoms improved temporarily, but three months later, psychiatric symptoms, i.e., suicidal thoughts and Cotard and Capgras delusions, were exaggerated. After ECT, her condition significantly improved. In conclusion, the present report suggests that pineal gland dysfunction due to anti-recoverin antibody or its cross-reactivity with neuron-specific calcium-binding proteins may contribute to the neuropsychiatric symptoms observed in anti-recoverin antibody-positive encephalitis and that ECT can be a viable treatment option if immunotherapy proves ineffective. Additionally, decreased rCBF in the prefrontal cortex may be associated with the clinical features of Capgras and Cotard delusions.
White matter hyperintensities in bipolar disorder: systematic review and meta-analysis
Authors: Silva Tânia , Nunes Cesar , Ribeiro Andreia , Santana Isabel , Cerejeira Joaquim
Published: January 2024

Background

White matter hyperintensities are lesions of presumed vascular origin associated with Cerebral small vessel disease. WMH are common findings that and are associated with increased risk of cognitive impairment and dementia. A higher prevalence of WMH has been also reported in patients with bipolar disorder (BD), although the evidence is conflicting.

Objective

To compare the prevalence of WMH in adults with BD, with the prevalence found in healthy controls.

Methods

We searched the Embase, Medline/PubMed, and references cited in articles retrieved on May 20, 2023. We included case-control studies that compared the prevalence of WMH in adult BD patients with the prevalence of WMH in healthy controls, using T2-weighted magnetic resonance imaging. We performed a meta-analysis using a random-effects method based on the inverse-variance approach.

Findings

We included 22 case-control studies reporting data of 1313 people. The overall rate of WMH was 46.5% in BD patients and 28% in controls (pooled Odds Ratio 2.89, 95% CI 1.76; 4.75). We found a moderate heterogeneity across studies (I2 = 0.49). Publication bias was not significant.

Interpretation

We found evidence that BD patients have a higher burden of WMH than healthy controls. Main limitations were impossibility of analyzing gender differences and bipolar type, moderate heterogeneity between studies, non-representative samples, lack of control for major confounders and search in two electronic databases.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42023428464
Knowledge, attitudes, and practices regarding cardiovascular disease prevention among middle school students in China: a cross-sectional study
Authors: Yang Xin , Qin Qiang , Wang Yifei , Ma Zhaopeng , Li Qiurong , Zhang Fusheng , Han Yanbai , Wang Hongli
Published: January 2024

Background

The prevalence of cardiovascular disease (CVD) is rapidly increasing globally. With a concerning increase among adolescents due to unhealthy habits, obesity, and hypertension, understanding the current status of knowledge, attitudes, and practices (KAP) related to CVD prevention among middle school students is crucial for developing effective school-based health programs to prevent CVD.

Methods

The analytic cross-sectional survey is used in questionnaires to assess KAP related to CVD prevention among middle school students (N = 17,731) from 50 schools across 16 provinces in China in June–July 2023. The pass rate of KAP scores is categorized as good and poor. Independent predictors of good KAP of CVD prevention are ascertained using a binary logistic regression model.

Results

The study surveyed 8,118 (45.78%) junior high school students and 9,613 (54.22%) high school students. The overall mean [standard deviation (SD)] for the knowledge, attitude, and practice scores were 26.88 (8.12), 53.53 (7.22), and 39.80 (5.96), respectively. The knowledge scores had the lowest pass rate at 56.89%. Only 6.83% of the students know “the definition of blood pressure in adolescents.” Attitudes toward health were positive, though the attitude regarding “the danger of prolonged sedentary to cardiovascular health” scored lowest at 73.55%. The practice section had a pass rate of 89.30%; 40.27% of students reported that they spend more than an hour a day on screens. Only one-third of the students would go to bed before 12 o’clock. In univariate analysis, junior high school and high school students differed significantly in knowledge and practice (p < 0.001), but attitude did not differ significantly (p = 0.103).

Conclusion

The majority of students lack sufficient knowledge about CVD. It is also found that socioeconomic background, family environment, and educational levels have an impact on cardiovascular health behaviors among students. Strengthening health education involving students, parents, teachers, and communities is essential to promote health knowledge and practices among adolescents.
Unlocking longevity: the role of telomeres and its targeting interventions
Authors: Schellnegger Marlies , Hofmann Elisabeth , Carnieletto Martina , Kamolz Lars-Peter
Published: January 2024

Average life expectancy has been steadily increasing in developed countries worldwide. These demographic changes are associated with an ever-growing social and economic strain to healthcare systems as well as society. The aging process typically manifests as a decline in physiological and cognitive functions, accompanied by a rise in chronic diseases. Consequently, strategies that both mitigate age-related diseases and promote healthy aging are urgently needed. Telomere attrition, characterized by the shortening of telomeres with each cell division, paradoxically serves as both a protective mechanism and a contributor to tissue degeneration and age-related ailments. Based on the essential role of telomere biology in aging, research efforts aim to develop approaches designed to counteract telomere attrition, aiming to delay or reduce age-related diseases. In this review, telomere biology and its role in aging and age-related diseases is summarized along with recent approaches to interfere with telomere shortening aiming at well- and healthy-aging as well as longevity. As aging research enters a new era, this review emphasizes telomere-targeting therapeutics, including telomerase activators and tankyrase inhibitors, while also exploring the effects of antioxidative and anti-inflammatory agents, along with indirectly related approaches like statins.
Connections of bullying experienced by Kyokushin karate athletes with the psychological state: is “a Cure for Bullying” safe?
Authors: Vveinhardt Jolita , Kaspare Magdalena
Published: January 2024

Although some authors propose practicing martial arts as a prevention against bullying, little is known about bullying among karate athletes and the consequences of negative behaviour for athletes' psychological state. This study aims to explore the effect of bullying on the psychological state of Kyokushin karate athletes. A total of 371 athletes were surveyed to measure bullying experiences and signs of stress, anxiety, and depression. It was determined that 75.5% of Kyokushin karate athletes had experienced unethical behaviour by their coaches or other athletes towards them at least once, and the scores on the signs of stress, anxiety, and depression correlated with damage in the fields of communication, social relations, and physical health. The results of binary logistic regression have shown that the qualifications of karate athletes and their participation in competitions influence the risk of being bullied. Organisations in which Kyokushin karate athletes do sports should devote more attention to preventing bullying among karate athletes themselves, especially when preparing for competitions as bullying can harm communication, social relations, and physical health.
Late-Onset Exudative Pleural Effusions Without Concomitant Airway Obstruction or Lung Parenchymal Abnormalities: A Novel Presentation of Chronic Lung Allograft Dysfunction
Authors: Sindu Devika , Bansal Sandhya , Buddhdev Bhuvin , McAnally Kendra , Mohamed Hesham , Walia Rajat , Mohanakumar Thalachallour , Tokman Sofya
Published: January 2024

Restrictive allograft syndrome (RAS) is an aggressive variant of CLAD characterized by progressive restrictive ventilatory decline and persistent pleuro-parenchymal changes that can be seen on chest CT. We identified four lung transplant recipients with a progressive restrictive ventilatory defect due to lymphocyte-predominant exudative pleural effusions, but no pleuro-parenchymal abnormalities typical of RAS. Using molecular analysis, we also found increased levels of previously described immune markers of RAS, including NFkB, 20S proteasome, lipocalin, TNFα, and TGFβ, within the circulating small extracellular vesicles of the remaining living lung transplant recipient. Despite the absence of lung parenchymal changes, these patients had a poor prognosis with rapid deterioration in allograft function and no response to pleural-based interventions such as thoracentesis, decortication, and pleurodesis. We hypothesize that these cases represent a distinct CLAD phenotype characterized by progressive restriction due to pleural inflammation, lymphocyte-predominant pleural effusion, resultant compressive atelectasis, and eventual respiratory failure in the absence of lung parenchymal involvement.
Performance Evaluation of Ammonium Dinitramide-Based Monopropellant in a 1N Thruster
Authors: Yoon Wonjae , Bhosale Vikas Khandu , Yoon Hosung
Published: January 2024

The development of propulsion systems based on green propellants, as an alternative to hydrazines, has been gaining interest within the space community. The study of Ammonium Dinitramide (ADN)-based liquid monopropellant, which is low-toxic and can deliver high performance, is the focal point of interest for Space Solutions Co., Ltd., Daejeon, Republic of Korea. A 1N ADN-based propulsion system was designed to evaluate the performance of the propellant. By combining a thermal and catalytic bed in a reactor, the performance of the propellant was examined in a designed thruster (chamber pressure of 11 bar). A total of 16 tests, with pulse mode experiments, were conducted; the accumulated firing time was 285 s. The preheating temperatures were maintained between 350 and 400 °C to achieve steady-state combustion. Notably, the maximum combustion efficiency was 91%. Test 9 recorded the highest decomposition temperature of propellant in the catalyst bed (1422 °C). Interestingly, the combustion instability observed throughout this study was ≤0.5%. This study could assist in the further development of ADN-based propulsion systems.
Potential Geographical Distribution of Lagerstroemia excelsa under Climate Change
Authors: Hao Siwen , Zhang Donglin , Wen Yafeng
Published: January 2024

As a popular ornamental plant and an effective species for controlling rocky desertification, the identification and protection of potential habitats of Lagerstroemia excelsa habitats hold significant importance. To gain a comprehensive understanding of the natural resources and growing conditions for L. excelsa, predictive modeling was employed to estimate the potential geographical distribution of the species during the Mid-Holocene (MH), the present, and the years 2050 and 2070. The projection was based on current occurrences, and we selected the relevant environmental attributes through the Pearson analysis and the Maximum Entropy Model (MaxEnt). The analysis revealed that temperature and precipitation are the primary environmental factors influencing L. excelsa distribution, with the Wuling Mountains identified as a center distribution hub for this species. The anticipated suitable area for L. excelsa is expected to experience marginal expansion under future climate scenarios. These results are invaluable for guiding the protection and sustainable utilization of L. excelsa in the face of climate change. Additionally, the data generated can be leveraged for enhanced introduction, breeding, selection, and cultivation of L. excelsa, taking into account the challenges posed by global warming.
Flowering and Flower Development in Plants
Authors: Chen Min , Zhang Jin-Zhi
Published: January 2024

In the life cycle of a plant, flowering marks the transition from vegetative growth to reproductive development [...]
Origanum dictamnus Essential Oil in Vapour or Aqueous Solution Application for Pepper Fruit Preservation against Botrytis cinerea
Authors: Tzortzakis Nikolaos
Published: January 2024

The use of synthetic sanitizers for fresh commodities preservation is of concern, with eco-friendly alternatives, including essential oils (EOs), attracting research and industry interest. Dittany (Origanum dictamnus—DIT) oil was applied, either through vapour or dipping, on pepper fruit or in vitro against Botrytis cinerea, and compared to untreated (control) or chlorine (CHL)-treated fruits stored at 11 °C. Direct DIT vapours (up to 6 d) suppressed lesion growth, spore germination, and spore production compared to the untreated fruits. The antimicrobial properties of EOs were evidenced in pre-exposed-to-DIT oil vapours (residual effect), resulting in fruit lesion suppression. However, DIT-pretreated fruits had the same spore production and spore germination as the control and CHL applications. In in vitro tests, DIT vapours decreased colony growth and spore production when fungi were grown on Potato Dextrose Agar (PDA) or PDA pre-exposed to DIT following B. cinerea inoculation. This evidenced that the disease suppression after DIT vapour application primarily affected the interaction of the fruit–pathogen and/or residual responses on fruit tissue and/or PDA media. Fungal biomass in Potato Dextrose Broth (PDB) was evaluated after DIT and CHL applications (10–50–100–500–5000 μL L−1) and decreased with the DIT oil. Additionally, DIT or CHL sanitary dipping on pepper was ineffective against B. cinerea compared to vapour application. Therefore, DIT vapours revealed antimicrobial properties and could be an alternative postharvest sanitiser. DIT oil application should also be evaluated at semi-commercial scale for further optimizations, prior to commercialisation.
Structural and Functional Analysis of the Bacterial Community in the Soil of Continuously Cultivated Lonicera japonica Thunb. and Screening Antagonistic Bacteria for Plant Pathogens
Authors: Ma Qingyun , Li Lin , Han Xiaoyan , Jiang Xu , Zhou Yiqing , Wang Tan , Zhuang Yan , Ruan Zhiyong
Published: January 2024

Continuous cropping is the main form of cultivation in Chinese agriculture. The bacterial community plays an important role in maintaining the healthy growth of plants. However, there are few reports on the composition and dynamics of the bacterial community structure under continuous cropping of Lonicera japonica Thunb. High-throughput sequencing was used to monitor the variation in the soil bacterial community structure of different monocropping years of Lonicera japonica Thunb., as well as the correlation between soil characteristics and bacterial community. Meanwhile, antagonistic bacteria for Fusarium oxysporum pathogens were isolated and functionally verified by culture-dependent techniques and pot experiments. Bacterial community diversity and structure changed significantly with the increase in the years of Lonicera japonica Thunb. succession. However, some beneficial bacteria, such as Bacillus and Nitrosospira, were gradually depleted. The complexity of the bacteria co-occurrence networks decreased with increasing years of cropping. FAPROTAX-based functional prediction showed that the abundance of genes related to carbon, nitrogen, sulfur metabolism and chitinlysis were reduced with the extended crop succession. Furthermore, the three Bacillus strains that were strongly antagonistic toward Fusarium oxysporum and the pot experiment demonstrated they significantly promoted Lonicera japonica Thunb. growth. Our research provides theoretical support for the development of microbial fertilizers that are beneficial to plants.
Status of Soil Health Indicators after 18 Years of Systematic Tillage in a Long-Term Experiment
Authors: Ibrahim Hanaa Tharwat Mohamed , Modiba Maxwell Maimela , Dekemati Igor , Gelybó Györgyi , Birkás Márta , Simon Barbara
Published: January 2024

Long-term cultivation experiments are gaining more attention due to the possibility of following the changes in soil parameters (e.g., soil organic carbon (SOC), stock and soil health indicators, etc.). Our objective was to assess the status of soil in an 18-year-old tillage experiment after almost two decades of systematic tillage. In this research, soil physical (bulk density, moisture content), chemical (pH, SOC), and biological properties (soil microbial respiration, abundance, biomass, species composition of earthworms, yield) were used as indicators in three soil cultivation methods representing different degrees of disturbance (no-till—NT; shallow cultivation—SC; and ploughing—P). Based on our results, there were significant differences in bulk density (NT > SC, P) in 0–10 cm, and NT > P in deeper layers (10–20, 20–30, 30–40 cm), while the SOC content in 0–10 cm was the highest in NT (2.5%), followed by SC (2.4%) and P (2.0%). Soil microbial respiration was significantly greater in NT than in SC and P. The abundance and biomass of earthworms was the highest in NT (189 ind m−2, 41.26 g m−2), followed by SC (125 ind m−2, 36.9 g m−2) and P (48 ind m−2, 7.4 g m−2). We concluded that NT offered a beneficial habitat for earthworms and microorganisms and a high SOC storage capacity; however, bulk density was less convenient due to soil compaction in our experiment. Therefore, SC can be used as an alternative approach for sustainable soil tillage.
Comparing Spatial Sampling Designs for Estimating Effectively Maize Crop Traits in Experimental Plots
Authors: Koutsos Thomas M. , Menexes Georgios C.
Published: January 2024

The current study investigates the performance of various sampling designs in providing accurate estimates for crucial maize yield traits (intended for silage) including plant height, fresh/dry/ear weight, number of maize ears per plant, and total ear weight per plant, using spatial maize data. The experiment took place in an experimental field area at Aristotle University (AUTH) farm during the 2016 growing season. Nine sampling designs were statistically analyzed and compared with spatial data from an Italian maize hybrid (AGN720) to identify the most suitable and effective sampling design for dependable maize yield estimates. The study’s results indicate that, among the different sampling techniques, Stratified Random Sampling is the most effective and reliable method for obtaining accurate maize yield estimates. This new approach not only provides precise estimates but also requires fewer measurements, making it suitable for experiments where not all plants have emerged. These findings suggest that Stratified Random Sampling can be employed effectively as an alternative to harvesting the entire plot for effectively estimating maize crop traits in experimental plots.
The Potential of Wood Vinegar to Replace Antimicrobials Used in Animal Husbandry—A Review
Authors: Gama Gil Sander Próspero , Pimenta Alexandre Santos , Feijó Francisco Marlon Carneiro , Azevedo Tatiane Kelly Barbosa de , Melo Rafael Rodolfo de , Andrade Gabriel Siqueira de
Published: January 2024

The indiscriminate use of antimicrobials in animal husbandry can result in various types of environmental contamination. Part of the dose of these products is excreted, still active, in the animals’ feces and urine. These excreta are widely used as organic fertilizers, which results in contamination with antimicrobial molecules. The impacts can occur in several compartments, such as soil, groundwater, and surface watercourses. Also, contamination by antimicrobials fed or administrated to pigs, chickens, and cattle can reach the meat, milk, and other animal products, which calls into question the sustainability of using these products as part of eco-friendly practices. Therefore, a search for alternative natural products is required to replace the conventional antimicrobials currently used in animal husbandry, aiming to mitigate environmental contamination. We thus carried out a review addressing this issue, highlighting wood vinegar (WV), also known as pyroligneous acid, as an alternative antimicrobial with good potential to replace conventional products. In this regard, many studies have demonstrated that WV is a promising product. WV is a nontoxic additive widely employed in the food industry to impart a smoked flavor to foods. Studies have shown that, depending on the WV concentration, good results can be achieved using it as an antimicrobial against pathogenic bacteria and fungi and a valuable growth promoter for poultry and pigs.
A Mini-Review of the Main Effects of Essential Oils from Citrus aurantifolia, Ocimum basilicum, and Allium sativum as Safe Antimicrobial Activity in Poultry
Authors: Oliveira Gabriel da Silva , McManus Concepta , Sousa Heloisa Alves de Figueiredo , Santos Pedro Henrique Gomes de Sá , dos Santos Vinícius Machado
Published: January 2024

Poultry production is accompanied by the use of antimicrobial agents because no production step is free of microorganisms. In the absence of antimicrobial treatments with synthetic drugs, essential oils are among the most cited natural alternatives used to prevent and treat microbial contamination in poultry. Although there are several studies on the antimicrobial properties of essential oils, there is still no review that simultaneously compiles information on the leading antimicrobial role of essential oils from Citrus aurantifolia (CAEO), Ocimum basilicum (OBEO), and Allium sativum (ASEO) in poultry. Awareness of the antimicrobial role of these substances opens the door to encouraging their use in natural antimicrobial protocols and discouraging harmful synthetics in poultry. This review aimed to compile information on applying CAEO, OBEO, and ASEO as antimicrobials in poultry farming. The available literature suggests that these essential oils can proportionately align with the poultry industry’s demands for microbiologically safe food products.
Factors Associated with Reproductive Success in Captive Vancouver Island Marmots (Marmota vancouverensis)
Authors: Graham Laura H. , Leishman Emily M. , Demers Kahlee , Whiteside Douglas P. , McAdie Malcolm
Published: January 2024

The Vancouver Island marmot (Marmota vancouverensis) is Canada’s most endangered endemic mammal. In 1997, a conservation breeding-for-release program was established to supplement wild marmot populations. Retrospective analyses of captive breeding studbook records since 2000 indicate the age of the sire and the dam significantly impacted the odds of successfully weaning a litter. Dams and sires between 5 and 7 years of age had more than double the odds of reproductive success compared to older animals. Successful reproduction by the dam in the previous year also doubled the odds of successfully weaning a litter in subsequent years. Assessment of adrenal function via fecal glucocorticoid analyses indicated established breeding pairs had decreased stress compared to new pairs (5.74 ± 0.28 ng/g vs. 7.60 ± 0.34 ng/g; p < 0.0001). Pairs that were ultimately successful at weaning pups in a breeding season had decreased stress compared to unsuccessful pairs (6.05 ± 0.34 ng/g vs. 7.22 ± 0.28 ng/g; p = 0.0006). These endocrine results suggest social buffering via familiarity and breeding/pair bond formation may be decreasing stress in established and successful pairs, respectively. The results of this study will be used to assist in the captive breeding management of this species to optimise numbers of animals produced to supplement the wild populations.
Characterization and Spike Gene Analysis of a Candidate Attenuated Live Bovine Coronavirus Vaccine
Authors: Park Gyu-Nam , Choe SeEun , Song Sok , Kim Ki-Sun , Shin Jihye , An Byung-Hyun , Moon Soo Hyun , Hyun Bang-Hun , An Dong-Jun
Published: January 2024

The bovine coronavirus (BCoV) KBR-1 strain, obtained from calf diarrhea samples collected in 2017, belongs to group GIIa. To attenuate this strain, it was subcultured continuously (up to 79 times) in HRT-18 cells, followed by 80–120 passages in MDBK cells. The KBR-1-p120 strain harvested from MDBK cells at passage 120 harbored 13 amino acid mutations in the spike gene. Additionally, the KBR-1-p120 strain showed a high viral titer and cytopathogenic effects in MDBK cells. Seven-day-old calves (negative for BCoV antigen and antibodies) that did not consume colostrum were orally inoculated with the attenuated candidate strain (KBR-1-p120), or with KBR-1 passaged 10 times (KBR-1-p10) in HRT-18 cells. Calves inoculated with KBR-1-p10 had a low diarrhea score, and BCoV RNA was detected at 3–7 days post-inoculation (DPI). The virus was also present in the duodenum, jejunum, and ileum at autopsy; however, calves inoculated with KBR-1-p120 had low levels of BCoV RNA in feces at 4–6 DPI, and no diarrhea. In addition, an extremely small amount of BCoV RNA was present in the jejunum and ileum at autopsy. The small intestines of calves inoculated with KBR-1-p120 were emulsified and used to infect calves two more times, but pathogenicity was not recovered. Therefore, the KBR-1-p120 strain has potential as a live vaccine candidate.
Automated Hematological Approach and Protein Electrophoretic Pattern in Tilapia (Oreochromis niloticus): An Innovative and Experimental Model for Aquaculture
Authors: Fazio Francesco , Costa Antonino , Capparucci Fabiano , Costa Gregorio , Parrino Vincenzo , Arfuso Francesca
Published: January 2024

This study aimed to assess the usefulness of two innovative automated methods (automated blood count counters and flow cytometry) for hematological investigation in Tilapia to make a contribution to the clinical diagnostics of this farmed species. Moreover, serum total proteins and their electrophoretic fractions (prealbumin, albumin, α-, β-, and γ-fraction), as health condition indicators, were assessed. The analysis of serum total proteins and electrophoretic fraction showed a normal and typical electrophoretic pattern of healthy fish (serum total proteins, 3.70 ± 0.62 g/dL; prealbumin, 0.44 ± 0.20 g/dL; albumin, 1.17 ± 0.66 g/dL; α-fraction, 1.49 ± 0.64 g/dL; β-fraction, 0.32 ± 0.16 g/dL; and γ-fraction, 0.29 ± 0.13 g/dL). The relationships between the values of red blood cells (RBCs), white blood cells (WBCs), and thrombocytes (TCs) obtained with the two automated methods were determined using Pearson correlation analysis. The results showed a significant positive correlation between automatic blood cell counting and flow cytometry analysis for RBCs (r = 0.97, p < 0.0001) and WBCs (r = 0.91, p < 0.0001), whereas no correlation was found for TCs (r = −0.11, p = 0.66). The preliminary results gathered in this study seem to highlight the usefulness of the new analytical techniques herein investigated in tilapia, suggesting their application in the hematological investigation of farmed fish species and their usefulness for monitoring the health and well-being of fish reared in aquaculture.
What Do We Know about Dog Owners? Exploring Associations between Pre-Purchase Behaviours, Knowledge and Understanding, Ownership Practices, and Dog Welfare
Authors: Philpotts Izzie , Blackwell Emily J. , Dillon Justin , Tipton Emma , Rooney Nicola J.
Published: January 2024

Despite many dogs living in homes in the UK, there is still more to know about the welfare of those individual animals. Past research has shown that owners’ thoughts and behaviours have a substantial impact on their dog’s welfare. This study aimed to better understand owners’ pre-purchase behaviours, knowledge and understanding, and ownership practices, and explore any associations between these factors and their dog’s welfare. We conducted further analysis of the data collected by People’s Dispensary for Sick Animals (PDSA) for their 2017 PDSA Animal Welfare (PAW) Report (n = 1814). We extracted variables to describe owner demographics (7), pre-purchase behaviours (1), knowledge and understanding (4), welfare indicators, (2) and ownership practices (4), and we tested for an association between these variables. We found more pre-purchase research was carried out by younger respondents and those with a higher education level. Also, more research was associated with feeling more informed about the five welfare needs and knowing to seek help for behaviour from appropriate sources. Overall, the study found several novel and significant results worthy of note and further exploration but did not find any strong connections between the variables.
Effect of Guanidinoacetic Acid Supplementation on Growth Performance, Rumen Fermentation, Blood Indices, Nutrient Digestion, and Nitrogen Metabolism in Angus Steers
Authors: Yi Simeng , Hu Sanlong , Wang Jinze , Abudukelimu Abudusaimijiang , Wang Yao , Li Xiang , Wu Hao , Meng Qingxiang , Zhou Zhenming
Published: January 2024

Guanidinoacetic acid (GAA) functions as a precursor for creatine synthesis in the animal body, and maintaining ample creatine reserves is essential for fostering rapid growth. This study aimed to explore the impact of GAA supplementation on growth performance, rumen fermentation, blood indices, nutrient digestion, and nitrogen metabolism in Angus steers through two experiments: a feeding experiment (Experiment 1) and a digestive metabolism experiment (Experiment 2). In Experiment 1, thirty-six Angus steers (485.64 ± 39.41 kg of BW) at 16 months of age were randomly assigned to three groups: control (CON), a conventional dose of GAA (CGAA, 0.8 g/kg), and a high dose of GAA (HGAA, 1.6 g/kg), each with twelve steers. The adaptation period lasted 14 days, and the test period was 130 days. Weighing occurred before morning feeding on days 0, 65, and 130, with rumen fluid and blood collected before morning feeding on day 130. Experiment 2 involved fifteen 18-month-old Angus steers (575.60 ± 7.78 kg of BW) randomly assigned to the same three groups as in Experiment 1, with a 7-day adaptation period and a 3-day test period. Fecal and urine samples were collected from all steers during this period. Results showed a significantly higher average daily gain (ADG) in the CGAA and HGAA groups compared to the CON group (p = 0.043). Additionally, the feed conversion efficiency (FCE) was significantly higher in the CGAA and HGAA groups than in the CON group (p = 0.018). The concentrations of acetate and the acetate:propionate ratio were significantly lower in the CGAA and HGAA groups, while propionate concentration was significantly higher (p < 0.01). Serum concentration of urea (UREA), blood ammonia (BA), GAA, creatine, and catalase (CAT) in the CGAA and HGAA groups were significantly higher than in the CON group, whereas malondialdehyde (MDA) concentrations were significantly lower (p < 0.05). Digestibility of dry matter (DM) and crude protein (CP) and the nitrogen retention ratio were significantly higher in the CGAA and HGAA groups than in the CON group (p < 0.05). In conclusion, dietary addition of both 0.8 g/kg and 1.6 g/kg of GAA increased growth performance, regulated rumen fermentation and blood indices, and improved digestibility and nitrogen metabolism in Angus steers. However, higher doses of GAA did not demonstrate a linear stacking effect.