bluuebunny/crossref_metadata_2025 huggingface dataset:
Tm3+: Bi4Si3O12crystal as a promising laser material near 2 μm: growth, spectroscopic properties and laser performance
Authors: Zhang Yan , Huang Liwu , Duan Xiaoming , Gong Zheng , Tian Tian , Ma Yunfeng , Xu And Jiayue
Published: August 2021

We report on the crystal growth, spectroscopic properties and laser performance of Tm3+-doped Bi4Si3O12(BSO) crystal. The crystal was grown by the vertical Bridgeman method. The spectroscopic properties are investigated based on absorption and luminescence spectroscopy. Judd - Ofelt (JO) analysis is performed to calculate the spontaneous emission probabilities, branching ratio and the radiative lifetimes. The absorption spectrum, emission spectrum and gain cross-section spectra of Tm3+: BSO crystal are determined for the 2 μm transition. Luminescence decay kinetic of3F4upper level was analysed in detail. The continuous-wave 2 μm laser with a maximum output power of 650 mW and a slope efficiency of 29.7% is demonstrated for the first time. The beam quality factor (M2) of Tm3+: BSO laser was about 1.03 at the maximum output level.
Realization of Structural Colors via Capped Cu-based F−P Cavity Structure
Authors: Rahman M. A. , Kim Yun Hee , Cho So-Hye , Lee Seung Yong , Byun Ji Young
Published: August 2021

Structural colors with tunable properties have several applications in the beautification of mobile devices, surface decoration, art and color filters. Herein, we propose an asymmetric F−P cavity design to systematically tune structural colors by changing the thickness of the top metal and intermediate insulator. In this study, Cu and Si3N4 were chosen as the top metal and intermediate insulator layers, respectively, various reflection colors being realized on the Cu surface. Various capping layers—that is, SiO2, polymethyl methacrylate (PMMA), and a commercially available clear coat named ProtectaClear—were used to protect the Cu surface from scratching and oxidation. PMMA coatings can protect Cu from corrosive environments without degradation of the color quality. The colors can be tuned by controlling the thickness of either the metal or intermediate insulator layers, and vivid structural colors—including orange, bright orange, red, purple, violet, light blue, green-yellow, and yellow-green—can be printed. The colors obtained can be attributed to thin-film interference.
Quantum interference in anti-parity-time symmetric coupled waveguide system
Authors: Qin Yan , Chen Haixia , Luo Dajin , Pan Churong , Hu Huajie , Zhang Yingxin , Wei Dong
Published: August 2021

We theoretically demonstrate quantum interference in an anti-parity-time (anti-PT) symmetric system based on coupled waveguides. We calculate the coincidence probability in an input polarization-entangled two-photon state, which can be used to simulate different statistical particles. When the birefringence of the waveguides is negligible, our results indicate that the coincidence probabilities of the bosons and fermions decrease exponentially with the propagation distance in both the unbroken and broken anti-PT symmetry regions owing to the dissipation. Particularly, loss-induced transparency is observed for the bosons. Simultaneously, the statistical rule valid in the Hermitian system is violated and the antibunching of bosons is observed. When the birefringence of the waveguides is considered, the coincidence probability of the bosons and fermions is equalized at the exceptional point (EP), whereas that of the bosons is less(greater) than that of the fermions in the broken(unbroken) anti-PT symmetry region. Additionally, we observe the Hong-Ou-Mandel dip for bosons in the broken anti-PT phase. Our research provides a complementary technique for the manipulation of quantum interference compared with the PT symmetric system and may be applied in building quantum devices with anti-PT symmetric quantum mechanics.
Analytical method for diffraction analysis and design of perfect-electric-conductor backed graphene ribbon metagratings
Authors: Rahmanzadeh Mahdi , Khavasi Amin , Rejaei Behzad
Published: August 2021

Graphene-based gratings and metagratings have attracted great interest in the last few years because they could realize various multi-functional beam manipulation, such as beam splitting, focusing, and anomalous reflection in the terahertz (THz) regime. However, most of graphene-based metagratings are designed through numerical simulations, which are very time-consuming. In this paper, an accurate analytical method is proposed for diffraction analysis of a perfect electric conductor (PEC)–backed array of graphene ribbons. In contrast to previous analytical treatments, the proposed method can predict the electromagnetic performance of graphene ribbons not only in the subwavelength regime, but also for wavelengths shorter than the array constant. Results are obtained by first deriving the surface current density induced on graphene ribbons by an obliquely incident transverse-magnetic (TM) polarized plane wave. Closed-form expressions for reflection coefficients of diffracted orders are then obtained using the surface current distribution. We validate the proposed method through comparison with full-wave simulation results. Finally, a tunable beam splitter and a tunable retroreflector in the THz regime are designed using the method proposed. The designed structures have good power efficiency (80% for beam splitter and 90% for retroreflector). Moreover, their operating frequency and angle may be controlled by changing the bias voltage of graphene ribbons. The proposed method paves the path for analytical design of tunable metagratings with widespread potential for THz and optical beam-manipulation applications.
Chirped pulse amplification of an ultrahigh-repetition-rate Ti:sapphire frequency comb using a tapered semiconductor amplifier
Authors: Sakamoto Takashi , Yoshioka Kosuke
Published: August 2021

Chirped pulse amplification of a 1.6 GHz Ti:sapphire femtosecond
frequency comb was achieved using a GaAs-based tapered semiconductor
amplifier. The spectral component of 855–865 nm was coherently
amplified up to 379 mW at 2.5 A, corresponding to a peak power of
1.48 kW and an average power of 150 µW per mode. A chirped Bragg
grating was used for pulse stretching and compression, resulting in a

150

m
m

×

200

m
m

compact amplification system.
Competition between the amplified spontaneous emission and the
coherent signal was observed at high driving currents. A numerical
analysis was presented to account for the observed gain suppression.
This amplification method is useful for the realization of high
repetition rate frequency combs with a wider spectral range, including
the UV and mid-infrared regions.
Refractive index modulation in magnetophoresis of bioreaction induced self-assembled magnetic fluid
Authors: Ma Rende , Li Xiaoping , Cao Hongzhong , Wu Wendi , Man Zhongxiao , Xia Yunjie
Published: August 2021

We explore the refractive index (RI) modulation of bioreaction induced self-assembled magnetic fluid (SAMF) so as to facilitate the design of sensitive biosensors. Bovine serum albumin (BSA) is taken as a model protein to induce self-assembly of carboxylated magnetic nanoparticles (MNPs). A bidirectional magnetophoresis (BDMP) system is designed to generate uniform and large RI modulation to SAMF. A robust Mach–Zehnder interferometer compatible to the BDMP system is used for the RI detection. It is found that BDMP has greater ability in modulating the RI of magnetic fluid than the alignment of MNPs in a uniform magnetic field. The modulation depth of the RI increases with the increase of BSA concentration when applying a certain external magnetic field for a period of time. A larger magnetic field has greater ability to modulate the RI of SAMF. However, it may generate an over-modulation.
Red-emitting InP quantum dot micro-disk lasers epitaxially grown on (001) silicon
Authors: Luo Wei , Lin Liying , Huang Jie , Han Yu , Lau Kei May
Published: August 2021

Direct epitaxy of InP quantum dot (QD) lasers on silicon (Si) provides an on-chip red laser source for integrated Si photonics with different applications. Here, we demonstrate the first, to the best of our knowledge, InP QD lasers directly grown on (001) Si. Combining highly emissive InP QDs and a GaAs/Si template with low defect density, continuous-wave (CW) lasing of micro-disk lasers (MDLs) on Si is achieved at room temperature. The lowest threshold of MDLs on Si is

∼

500

n
W

, without considering the micro-disk surface absorption efficiency of the pump power. The MDLs grown on the native GaAs substrate with the same growth and fabrication process are compared using statistical data analysis. Similar material characterization results and device performances on these two substrates further confirm the performance of QD lasers on Si. This demonstration paves the way for future realization of integrated photonic circuits with red and near-infrared (NIR) lasers on Si.
Effective medium theory for photonic crystals with quadrupole resonances
Authors: Liu Fengming , Peng Pai , Du QiuJiao , Ke Manzhu
Published: August 2021

An effective medium theory is proposed to characterize two-dimensional dielectric photonic crystals (PCs) exhibiting quadrupole resonances. In addition to the effective permittivity and permeability associated with electric and magnetic dipoles, we obtain a local effective parameter to describe the contributions of quadrupole resonances by taking the low frequency limit of multiple-scattering theory. These effective parameters can be used to predict the characteristics of double-Dirac-cone PCs, showing good agreement with the numerical results. Moreover, we show that, after introducing the new effective parameter, the double-Dirac-cone PCs can be regarded as a generalization of the traditional double-zero-index metamaterials.
Quick and reliable colorimetric reflectometry for the thickness determination of low-dimensional GaS and GaSe exfoliated layers by optical microscopy
Authors: Gutiérrez Yael , Santos Gonzalo , Giangregorio Maria M. , Dicorato Stefano , Palumbo Fabio , Saiz José M. , Moreno Fernando , Losurdo Maria
Published: August 2021

Interest in gallium chalcogenides, i.e., gallium sulfide (GaS) and gallium selenide (GaSe), is growing rapidly due to its layered structure compatible with the fabrication of very thin layers by mechanical exfoliation and its wide band gap desirable for the design and fabrication of visible-UV optoelectronic devices. It is well known that the properties of these materials depend on their thickness; therefore, a facile and fast method is needed to infer the thickness of layered GaS and GaSe. Here, we report and validate a colorimetric method based on optical imaging for the quick and reliable quantitative determination of the thickness of exfoliated GaS and GaSe layers although it can be extended to other layered systems. For the validation of the method, the colorimetric computational estimate of the thickness is compared to the value obtained by atomic force microscopy. Further simulation of GaS and GaSe layers on different substrates of interest for different technological applications is provided as a quick guide for the rapid and reliable thickness determination of GaS and GaSe layers on various substrates.
A hidden Markov model reliably characterizes ketamine-induced spectral dynamics in macaque local field potentials and human electroencephalograms
Authors: Garwood Indie C. , Chakravarty Sourish , Donoghue Jacob , Mahnke Meredith , Kahali Pegah , Chamadia Shubham , Akeju Oluwaseun , Miller Earl K. , Brown Emery N.
Published: August 2021

Ketamine is an NMDA receptor antagonist commonly used to maintain general anesthesia. At anesthetic doses, ketamine causes high power gamma (25-50 Hz) oscillations alternating with slow-delta (0.1-4 Hz) oscillations. These dynamics are readily observed in local field potentials (LFPs) of non-human primates (NHPs) and electroencephalogram (EEG) recordings from human subjects. However, a detailed statistical analysis of these dynamics has not been reported. We characterize ketamine’s neural dynamics using a hidden Markov model (HMM). The HMM observations are sequences of spectral power in seven canonical frequency bands between 0 to 50 Hz, where power is averaged within each band and scaled between 0 and 1. We model the observations as realizations of multivariate beta probability distributions that depend on a discrete-valued latent state process whose state transitions obey Markov dynamics. Using an expectation-maximization algorithm, we fit this beta-HMM to LFP recordings from 2 NHPs, and separately, to EEG recordings from 9 human subjects who received anesthetic doses of ketamine. Our beta-HMM framework provides a useful tool for experimental data analysis. Together, the estimated beta-HMM parameters and optimal state trajectory revealed an alternating pattern of states characterized primarily by gamma and slow-delta activities. The mean duration of the gamma activity was 2.2s([1.7,2.8]s) and 1.2s([0.9,1.5]s) for the two NHPs, and 2.5s([1.7,3.6]s) for the human subjects. The mean duration of the slow-delta activity was 1.6s([1.2,2.0]s) and 1.0s([0.8,1.2]s) for the two NHPs, and 1.8s([1.3,2.4]s) for the human subjects. Our characterizations of the alternating gamma slow-delta activities revealed five sub-states that show regular sequential transitions. These quantitative insights can inform the development of rhythm-generating neuronal circuit models that give mechanistic insights into this phenomenon and how ketamine produces altered states of arousal.
Phylogenomic assessment of the role of hybridization and introgression in trait evolution
Authors: Wang Yaxuan , Cao Zhen , Ogilvie Huw A. , Nakhleh Luay
Published: August 2021

Trait evolution among a set of species—a central theme in evolutionary biology—has long been understood and analyzed with respect to a species tree. However, the field of phylogenomics, which has been propelled by advances in sequencing technologies, has ushered in the era of species/gene tree incongruence and, consequently, a more nuanced understanding of trait evolution. For a trait whose states are incongruent with the branching patterns in the species tree, the same state could have arisen independently in different species (homoplasy) or followed the branching patterns of gene trees, incongruent with the species tree (hemiplasy). Another evolutionary process whose extent and significance are better revealed by phylogenomic studies is gene flow between different species. In this work, we present a phylogenomic method for assessing the role of hybridization and introgression in the evolution of polymorphic or monomorphic binary traits. We apply the method to simulated evolutionary scenarios to demonstrate the interplay between the parameters of the evolutionary history and the role of introgression in a binary trait’s evolution (which we call xenoplasy). Very importantly, we demonstrate, including on a biological data set, that inferring a species tree and using it for trait evolution analysis in the presence of gene flow could lead to misleading hypotheses about trait evolution.
Coagulation abnormalities in Dengue fever infection: A systematic review and meta-analysis
Authors: Adane Tiruneh , Getawa Solomon
Published: August 2021

Background

Coagulation mechanisms are reported to be affected in dengue illness and evidenced by prolonged activated partial thromboplastin time (APTT) and prothrombin time (PT). The main aim of this systematic review and meta-analysis is to determine the magnitude of coagulation abnormalities among patients with dengue fever infection.

Method

This systematic review and meta-analysis were conducted per the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guideline. The Joana Brigg’s Institute (JBI) critical appraisal checklist was used for quality appraisal. STATA version 11 software was used for meta-analysis. The magnitude of coagulation abnormalities among dengue fever patients was determined by using a random-effects model. Subgroup and sensitivity analysis were performed to investigate the possible source of heterogeneity. Egger weighted regression tests were used to check the presence of publication bias among the included articles.

Result

Forty-two studies with a total of 12,221 dengue fever patients were eligible for meta-analysis in this study. Of which 22, 15, and 26 studies were used to determine the magnitude of prolonged APTT, PT, and thrombocytopenia, respectively. The magnitude of prolonged APTT and PT among patients with dengue fever infection were 42.91% (95% CI: 30.95, 54.87) I2 = 99.1% and 16.48% (95% CI: 10.95, 22.01) I2 = 97.0%, respectively. Besides, the magnitude of thrombocytopenia among dengue fever patients was 70.29% (95% CI: 62.69, 77.89) I2 = 99.3%. The magnitude of prolonged APTT in children and adults was 51.21% (95% CI: 24.54, 77.89) and 44.89% (95% CI: 28.32, 61.45), respectively. Similarly, the overall magnitude of prolonged PT in children and adults were 13.40% (95% CI: 6.09, 20.71) and 18.73% (95% CI: 7.49, 29.96), respectively.

Conclusion

The result of this study showed that there is a high magnitude of prolonged APTT and PT in dengue fever patients. Therefore, screening and early correction of coagulation abnormalities may be helpful to reduce further complications in those patients.
Infection with Blastocystis spp. and its association with enteric infections and environmental enteric dysfunction among slum-dwelling malnourished adults in Bangladesh
Authors: Fahim Shah Mohammad , Gazi Md. Amran , Hasan Md. Mehedi , Alam Md. Ashraful , Das Subhasish , Mahfuz Mustafa , Rahman M. Masudur , Haque Rashidul , Sarker Shafiqul Alam , Ahmed Tahmeed
Published: August 2021

Background

Blastocystis spp. (Blastocystis) is a widely distributed gastrointestinal protist frequently reported in countries with tropical and sub-tropical climate. We sought to determine the factors associated with Blastocystis infection and investigate its role on biomarkers of intestinal health among slum-dwelling malnourished adults in Bangladesh.

Methodology

Total 524 malnourished adults with a body mass index ≤18.5 kg/m2 were included in this analysis. Presence of Blastocystis in feces was evaluated by TaqMan Array Card assays.

Principal findings

Blastocystis was tested positive in 78.6% of the participants. Prevalence of infection with atypical strains of enteropathogenic Escherichia coli (aEPEC) (56% vs. 38%, p<0.001), and Trichuris trichiura (28% vs. 15%, p-value = 0.02) was significantly greater in adults with Blastocystis, while Giardia intestinalis was significantly lower (8% vs. 14%, p-value = 0.04) in Blastocystis positive adults. Malnourished adults who were living in households with high crowding index (aOR = 2.18; 95% CI = 1.11, 4.65; p-value = 0.03), and infected with aEPEC (aOR = 2.14; 95% CI = 1.35, 3.44; p-value = 0.001) and Trichuris trichiura (aOR = 1.97; 95% CI = 1.08, 3.77; p = 0.03) were more likely to be infected with Blastocystis. A significant negative relationship was observed between Blastocystis and fecal concentrations of alpha-1 antitrypsin (β = -0.1; 95% CI = -1.7, -0.1; p-value<0.001) and Reg1B (β = -3.6; 95% CI = -6.9, -3.0; p-value = 0.03).

Conclusions

The study findings suggest that the presence of Blastocystis in human intestine influences gut health and may have potential pathogenic role in presence of other pathogens.
Sexual, allometric and forest cover effects on giant anteaters’ movement ecology
Authors: Giroux Aline , Ortega Zaida , Oliveira-Santos Luiz Gustavo Rodrigues , Attias Nina , Bertassoni Alessandra , Desbiez Arnaud Léonard Jean
Published: August 2021

Knowing the influence of intrinsic and environmental traits on animals’ movement is a central interest of ecology and can aid to enhance management decisions. The giant anteater (Myrmecophaga tridactyla) is a vulnerable mammal that presents low capacity for physiological thermoregulation and uses forests as thermal shelters. Here, we aim to provide reliable estimates of giant anteaters’ movement patterns and home range size, as well as untangle the role of intrinsic and environmental drivers on their movement. We GPS-tracked 19 giant anteaters in Brazilian savannah. We used a continuous-time movement model to estimate their movement patterns (described by home range crossing time, daily distance moved and directionality), and provide an autocorrelated kernel density estimate of home range size. Then, we used mixed structural equations to integratively model the effects of sex, body mass and proportion of forest cover on movement patterns and home range size, considering the complex net of interactions between these variables. Male giant anteaters presented more intensive space use and larger home range than females with similar body mass, as it is expected in polygynous social mating systems. Males and females increased home range size with increasing body mass, but the allometric scaling of intensity of space use was negative for males and positive for females, indicating different strategies in search for resources. With decreasing proportion of forest cover inside their home ranges, and, consequently, decreasing thermal quality of their habitat, giant anteaters increased home range size, possibly to maximize the chances of accessing thermal shelters. As frequency and intensity of extreme weather events and deforestation are increasing, effective management efforts need to consider the role of forests as an important thermal resource driving spatial requirements of this species. We highlight that both intrinsic and environmental drivers of animal movement should be integrated to better guide management strategies.
Data driven high resolution modeling and spatial analyses of the COVID-19 pandemic in Germany
Authors: Schüler Lennart , Calabrese Justin M. , Attinger Sabine
Published: August 2021

The SARS-CoV-2 virus has spread around the world with over 100 million infections to date, and currently many countries are fighting the second wave of infections. With neither sufficient vaccination capacity nor effective medication, non-pharmaceutical interventions (NPIs) remain the measure of choice. However, NPIs place a great burden on society, the mental health of individuals, and economics. Therefore the cost/benefit ratio must be carefully balanced and a target-oriented small-scale implementation of these NPIs could help achieve this balance. To this end, we introduce a modified SEIRD-class compartment model and parametrize it locally for all 412 districts of Germany. The NPIs are modeled at district level by time varying contact rates. This high spatial resolution makes it possible to apply geostatistical methods to analyse the spatial patterns of the pandemic in Germany and to compare the results of different spatial resolutions. We find that the modified SEIRD model can successfully be fitted to the COVID-19 cases in German districts, states, and also nationwide. We propose the correlation length as a further measure, besides the weekly incidence rates, to describe the current situation of the epidemic.
Having female role models correlates with PhD students’ attitudes toward their own academic success
Authors: Gillooly Shauna N. , Hardt Heidi , Smith Amy Erica
Published: August 2021

Research indicates that increasing diversity in doctoral programs can positively affect students’ academic success. However, little research examines students’ responses to female scholars’ representation. The two studies presented here examine how students’ exposure to female academic role models shapes students’ attitudes toward their own academic success (i.e. self-efficacy). Such attitudes are critical because they predict student retention rates. In our first study, we randomly exposed 297 Ph.D. students in one academic discipline to either a gender-diverse (i.e. 30% female authors) or non-diverse syllabus in research methods (i.e. 10% female authors). We examined the effect of the intervention on students’ perceived likelihood of succeeding in the hypothetical course. Contrary to expectations derived from the literature, we found that increasing women’s representation in syllabi did not affect female students’ self-efficacy. Rather, male students expressed lower self-efficacy when evaluating the more gender-diverse syllabus. We also found that students’ attitudes toward diversity in academia predicted their reactions more strongly than did their own gender: gender-diverse syllabi reduced self-efficacy among those students unsupportive of diversity. In our second study, we analyzed non-interventional survey questions to examine the relationship between female role models and long-term academic self-efficacy. Analysis was observational and thus did not assess causality. We found that students with more role models have higher academic self-efficacy, irrespective of student and role model gender. Nonetheless, results also suggested that some students actively seek female role models: namely, female students, and particularly those valuing diversity. Our results ultimately suggest that exposure to female role models relates in surprising ways to Ph.D. students’ self-efficacy. Having more female role models correlates with greater expectations of academic success among certain groups of students, but with diminished expectations of academic success among other groups.
Northern bobwhite select for shrubby thickets interspersed in grasslands during fall and winter
Authors: Mosloff Alisha R. , Weegman Mitch D. , Thompson Frank R. , Thompson Thomas R.
Published: August 2021

Resource selection is a key component in understanding the ecological processes underlying population dynamics, particularly for species such as northern bobwhite (Colinus virginianus), which are declining across their range in North America. There is a growing body of literature quantifying breeding season resource selection in bobwhite; however, winter information is particularly sparse despite it being a season of substantial mortality. Information regarding winter resource selection is necessary to quantify the extent to which resource requirements are driving population change. We modeled bobwhite fall and winter resource selection as a function of vegetation structure, composition, and management from traditionally (intensively) managed sites and remnant (extensively managed) grassland sites in southwest Missouri using multinomial logit discrete choice models in a Bayesian framework. We captured 158 bobwhite from 67 unique coveys and attached transmitters to 119 individuals. We created 671 choice sets comprised of 1 used location and 3 available locations. Bobwhite selected for locations which were closer to trees during the winter; the relative probability of selection decreased from 0.45 (85% Credible Interval [CRI]: 0.17–0.74) to 0.00 (85% CRI: 0.00–0.002) as distance to trees ranged from 0–313 m. The relative probability of selection increased from near 0 (85% CRI: 0.00–0.01) to 0.33 (85% CRI: 0.09–0.56) and from near 0 (85% CRI: 0.00–0.00) to 0.51 (85% CRI: 0.36–0.71) as visual obstruction increased from 0 to 100% during fall and winter, respectively. Bobwhite also selected locations with more woody stems; the relative probability of selection increased from near 0.00 (85% CRI: 0.00–0.002) to 0.30 (85% CRI: 0.17–0.46) and near 0.00 (85% CRI: 0.00–0.001) to 0.35 (85% CRI: 0.22–0.55) as stem count ranged from 0 to 1000 stems in fall and winter, respectively. The relative probability of selection also decreased from 0.35 (85% CRI: 0.20–0.54) to nearly 0 (85% CRI: 0.00–0.001) as percent grass varied from 0 to 100% in fall. We suggest that dense shrub cover in close proximity to native grasslands is an important component of fall and winter cover given bobwhite selection of shrub cover and previously reported survival benefits in fall and winter.
Passive citizen science: The role of social media in wildlife observations
Authors: Edwards Thomas , Jones Christopher B. , Perkins Sarah E. , Corcoran Padraig
Published: August 2021

Citizen science plays an important role in observing the natural environment. While conventional citizen science consists of organized campaigns to observe a particular phenomenon or species there are also many ad hoc observations of the environment in social media. These data constitute a valuable resource for ‘passive citizen science’—the use of social media that are unconnected to any particular citizen science program, but represent an untapped dataset of ecological value. We explore the value of passive citizen science, by evaluating species distributions using the photo sharing site Flickr. The data are evaluated relative to those submitted to the National Biodiversity Network (NBN) Atlas, the largest collection of species distribution data in the UK. Our study focuses on the 1500 best represented species on NBN, and common invasive species within UK, and compares the spatial and temporal distribution with NBN data. We also introduce an innovative image verification technique that uses the Google Cloud Vision API in combination with species taxonomic data to determine the likelihood that a mention of a species on Flickr represents a given species. The spatial and temporal analyses for our case studies suggest that the Flickr dataset best reflects the NBN dataset when considering a purely spatial distribution with no time constraints. The best represented species on Flickr in comparison to NBN are diurnal garden birds as around 70% of the Flickr posts for them are valid observations relative to the NBN. Passive citizen science could offer a rich source of observation data for certain taxonomic groups, and/or as a repository for dedicated projects. Our novel method of validating Flickr records is suited to verifying more extensive collections, including less well-known species, and when used in combination with citizen science projects could offer a platform for accurate identification of species and their location.
Polycystin-1 is required for insulin-like growth factor 1-induced cardiomyocyte hypertrophy
Authors: Fernández Carolina , Torrealba Natalia , Altamirano Francisco , Garrido-Moreno Valeria , Vásquez-Trincado César , Flores-Vergara Raúl , López-Crisosto Camila , Ocaranza María Paz , Chiong Mario , Pedrozo Zully , Lavandero Sergio
Published: August 2021

Cardiac hypertrophy is the result of responses to various physiological or pathological stimuli. Recently, we showed that polycystin-1 participates in cardiomyocyte hypertrophy elicited by pressure overload and mechanical stress. Interestingly, polycystin-1 knockdown does not affect phenylephrine-induced cardiomyocyte hypertrophy, suggesting that the effects of polycystin-1 are stimulus-dependent. In this study, we aimed to identify the role of polycystin-1 in insulin-like growth factor-1 (IGF-1) signaling in cardiomyocytes. Polycystin-1 knockdown completely blunted IGF-1-induced cardiomyocyte hypertrophy. We then investigated the molecular mechanism underlying this result. We found that polycystin-1 silencing impaired the activation of the IGF-1 receptor, Akt, and ERK1/2 elicited by IGF-1. Remarkably, IGF-1-induced IGF-1 receptor, Akt, and ERK1/2 phosphorylations were restored when protein tyrosine phosphatase 1B was inhibited, suggesting that polycystin-1 knockdown deregulates this phosphatase in cardiomyocytes. Moreover, protein tyrosine phosphatase 1B inhibition also restored IGF-1-dependent cardiomyocyte hypertrophy in polycystin-1-deficient cells. Our findings provide the first evidence that polycystin-1 regulates IGF-1-induced cardiomyocyte hypertrophy through a mechanism involving protein tyrosine phosphatase 1B.
The role of vitamin D in subjective tinnitus—A case-control study
Authors: Nowaczewska Magdalena , Osiński Stanisław , Marzec Maria , Wiciński Michał , Bilicka Katarzyna , Kaźmierczak Wojciech
Published: August 2021

Regarding the high prevalence of vitamin D (25(OH)D) deficiency in the population and its possible association with ear diseases, we aimed to investigate the 25(OH)Dserum level in patients with subjective, nonpulsating tinnitus and its effect on tinnitus severity. The study included 201 tinnitus patients and 99 controls. Patient clinical information, including tinnitus characteristics and severity according to Tinnitus Handicap Inventory (THI), loudness assessed by Visual Analogue Scale (VAS), audiometry, and the blood level of vitamin D, was recorded. The level of 25(OH)D in tinnitus patients was significantly decreased compared with the controls (19.86 ± 7.53 and 27.43 ± 8.85 ng/ml, respectively; P value < 0.0001). More patients in the tinnitus group were deficient in vitamin D, compared with the controls (50.7% vs. 22.2% respectively, p < 0.0001). Tinnitus patients with a lower serum level of 25(OH)D (≤15 ng/dl) were significantly younger, had a higher degree of tinnitus severity measured with THI and VAS scales, had higher triglyceride and TSH levels, and a lower HDL level compared with individuals who had higher 25(OH)D level (>15 ng/dl). There was a strong correlation between the 25(OH)D level and THI. Our findings suggest that a large proportion of tinnitus patients suffers from vitamin D deficiency and that the vitamin D level correlates with tinnitus impact. We recommend a vitamin D assessment for all tinnitus patients.
Efficacy of green synthesized silver nanoparticles via ginger rhizome extract against Leishmania major in vitro
Authors: Mohammadi Mohsen , Zaki Leila , KarimiPourSaryazdi Amir , Tavakoli Pooya , Tavajjohi Atiyeh , Poursalehi Reza , Delavari Hamid , Ghaffarifar Fatemeh
Published: August 2021

Introduction

Leishmaniasis is a major public health problem that causes by parasite of the genus Leishmania. The pentavalent antimonial compounds that used for treatment are not safe or effective enough. The aim of the present study was preparation and evaluation of the efficacy of green synthesized silver nanoparticles against Leishmania major (L. major) in vitro.

Methods

To synthesis silver (Ag) nanoparticles (NPs), ginger extract was added to the 0.2mM AgNO3 aqueous solution (1:20). Effects of different concentrations of Ag-NPs on the number of L. major promastigotes were investigated using counting assay. The MTT test was applied to determine the toxicity of Ag-NPs on promastigotes of L. major, as well as, macrophage cells. Then, to evaluate the anti-amastigotes effects of Ag-NPs, parasites within the macrophages were counted by light microscope. Furthermore, to determine the induced apoptosis and necrotic effects of Ag-NPs on promastigotes, flow cytometry method was employed using annexin staining.

Results

The effect of Ag-NPs on promastigotes and amastigotes of L. major was effective and has a reverse relationship with its concentration. According to the results of anti-amastigote assay, the IC50 value of this nanoparticle was estimated 2.35 ppm after 72h. Also, Ag-NPs caused Programmed Cell Death (PCD) in promastigotes of L. major and showed 60.18% of apoptosis.

Discussion

Based on the mentioned results, it can be concluded that Ag NPs has a beneficial effect on promastigote and amastigote forms of L. major in vitro. Hence, these nanoparticles could be applied as promising antileishmanial agents for treatment of Leishmania infections.
The pace of hospital life: A mixed methods study
Authors: Long Janet C. , Pomare Chiara , Ellis Louise A. , Churruca Kate , Braithwaite Jeffrey
Published: August 2021

The pace-of-life hypothesis is a socio-psychological theory postulating that citizens of different cities transact the business of life at varying paces, and this pace is associated with a number of population level variables. Here we apply the pace-of-life hypothesis to a hospital context to empirically test the association between pace and patient and staff outcomes. As pressure on hospitals grow and pace increases to keep up with demand, is there empirical evidence of a trade-off between a rapid pace and poorer outcomes? We collected data from four large Australian hospitals, inviting all staff (clinical and non-clinical) to complete a survey, and conducted a series of observations of hospital staff’s walking pace and transactional pace. From these data we constructed three measures of pace: staff perception of pace, transactional pace, and walking pace. Outcome measures included: hospital culture, perceived patient safety, and staff well-being outcomes of job satisfaction and burnout. Overall, participants reported experiencing a “fast-paced” “hurried” and “rapid” pace-of-life working in the Australian hospital sector. We found a significant difference in perceived pace across four hospital sites, similar to trends observed for transactional pace. This provides support that the pace-of-life hypothesis may apply to the hospital context. We tested associations between faster perceived pace, hospital culture, staff well-being and patient safety. Results revealed perceived faster pace significantly predicted negative perceptions of organizational culture, greater burnout and lower job satisfaction. However, perceived pace did not predict perceptions of patient safety. Different perceptions of hospital pace-of-life were found between different clinical settings and the type of care delivered; staff working in emergency departments reported significantly “faster-paced” work environments than staff working in palliative, aged care, or rehabilitation wards.
Development of an alternative saliva test for diagnosis of SARS-CoV-2 using TRIzol: Adapting to countries with lower incomes looking for a large-scale detection program
Authors: Miranda-Ortiz Haydee , Fernández-Figueroa Edith A. , Ruíz-García Erika B. , Muñoz-Rivas Anallely , Méndez-Pérez Alejandra , Méndez-Galván Jorge , Astudillo-de la Vega Horacio , Gabiño-López Belem , Nava-Monroy Ricardo , López-Flores a la Torre Alejandro , López-Vergara Anaya Tanit , Vilar-Compte Diana , Coquis-Navarrete Uriel , Valdés-Reyes Melissa , Sánchez-Montes Sokani , Becker Ingeborg
Published: August 2021

The use of saliva for the diagnosis of SARS-CoV-2 has shown to be a good alternative to nasopharyngeal swabs (NPS), since it permits self-collection, avoids the exposure of healthy persons to infected patients, reduces waiting times, eliminates the need of personal protective equipment and is non-invasive. Yet current saliva testing is still expensive due to the need of specialized tubes containing buffers to stabilize the RNA of SARS-CoV-2 and inactivate the virus. These tubes are expensive and not always accessible in sufficient quantities. We now developed an alternative saliva testing method, using TRIzol for extraction, viral inactivation, and storage of SARS-CoV-2 RNA, combined with RT-qPCR, which was comparable in its performance to NPS. Paired saliva samples and NPS were taken from 15 asymptomatic healthcare workers and one patient with SARS-CoV-2. Further 13 patients with SARS-CoV-2 were only saliva-tested. All the tests were performed according to CDC 2019-Novel Coronavirus (2019-nCoV) Real-Time RT-PCR Diagnostic Panel. Saliva (4 mL) was taken in sterile 50 mL tubes, 1.5 mL TRIzol were added and mixed. Our results show that 5 μL of saliva RNA extracted with TRIzol allow for an adequate detection of the virus in patients positive for SARS-CoV-2 and was equally sensitive to NPS in TRIzol. We conclude that saliva testing using TRIzol is a recommendable method for diagnosis of SARS-CoV-2 since it has several advantages over currently used saliva tests: it can be done with normal sterile tubes, does not need cold-chain handling, is stable at room temperature, is non-invasive and less costly, making it more accessible for low-income countries. Cheaper saliva testing using TRIzol is especially relevant for low-income countries to optimize diagnosis and help define quarantine durations for families, healthcare workers, schools, and other public workplaces, thus decreasing infections and mortality caused by SARS-CoV-2.
Association of leptin G2548A and leptin receptor Q223R polymorphisms and their serum levels with infertility and recurrent pregnancy loss in Iranian women with polycystic ovary syndrome
Authors: Kargasheh Fatemeh Bagheri , Ansaripour Soheila , Borumandnia Nasrin , Moradi Nariman , Zandieh Zahra , Maleki Monireh , Mokhtar Sara , Karimi Atousa , Fatemi Farnaz , Kheirollahi Asma , Vatannejad Akram
Published: August 2021

Background

Adipokine leptin plays a crucial role in metabolic and reproductive functions. Leptin receptor has a soluble form that binds to leptin, thus modulating its level in the circulation. It has been indicated that the levels of leptin and leptin receptor and also LEP rs7799039 and LEPR rs1137101 polymorphisms are associated with metabolic disorders. In the present study, we assessed the levels of leptin and soluble leptin receptor (sOB-R), and also the frequency of rs7799039 and rs1137101 polymorphisms in healthy fertile women and patients with polycystic ovary syndrome (PCOS), inclusive of PCOS-infertile and PCOS-recurrent pregnancy loss (RPL) subjects.

Methods

A total of 324 PCOS patients- including 199 infertile cases and 125 patients with a history of RPL- and 144 healthy controls were enrolled in this study. Biochemical parameters and plasma leptin and sOB-R levels were measured by ELISA and the genotypes of rs7799039 and rs1137101 polymorphisms were determined using PCR- RFLP.

Results

Plasma leptin and sOB-R levels were significantly higher and lower in PCOS, PCOS-infertile and PCOS RPL groups, respectively. The GG genotype frequencies of rs7799039 and rs1137101 polymorphisms were significantly different between PCOS-infertile women and non-PCOS subjects (P = 0.043, OR = 0.47, 95% CI = 0.22–0.97, and P = 0.01, OR = 0.31, 95% CI = 0.12–0.75, respectively). Increased LEP levels were associated with the risk of PCOS and RPL in women with PCOS (P = 0.039, OR = 1.203, 95%CI = [1.009–1.435] and P = 0.012, OR = 1.267, 95% CI = [1.054–1.522], respectively).

Conclusion

Polymorphisms rs7799039 and rs1137101 and circulating leptin and sOB-R levels were associated with infertility in Iranian women with PCOS. Further studies are needed to reveal the role of leptin in PCOS pathogenesis.
Recommending blue ocean technologies for subcontractors: A framework based on business reports of prime contractors and patents
Authors: Woo Hyunwoo , Lim Sun Woo , Sohn So Young
Published: August 2021

Subcontractors depend heavily on their prime contractor and thus find it very risky to enter a new business on their own. This study proposes a framework for these subcontractors to develop blue ocean technologies related to their prime contractor. First, the primary technologies predicted to be promising are extracted from the business reports of the prime contractor. Sub-technologies are then selected through a patent-based search using keywords and International Patent Classification codes of the primary technologies. From them, blue ocean technologies are proposed by optimizing the weighted mean of the min-max normalized market value, degree of competition in the technology market, and subcontractors’ potential technological capabilities for each sub-technology. This study shows that subcontractors can enhance their technology competitiveness by finding a low-risk blue ocean technology. Our empirical research on the subcontractors of a semiconductor firm identified technological patent fields for them to pursue. From our framework, subcontractors can identify blue ocean technologies by considering their prime contractor’s future industrial areas and technologies of interest as well as their own technological capabilities. Furthermore, the prime contractors can gain the synergy effect of technology expansion through cooperation.
Exploring the acute affective responses to resistance training: A comparison of the predetermined and the estimated repetitions to failure approaches
Authors: Schwartz Hadar , Emanuel Aviv , Rozen Samukas Isaac Isur , Halperin Israel
Published: August 2021

Background

In resistance-training (RT), the number of repetitions is traditionally prescribed using a predetermined approach (e.g., three sets of 10 repetitions). An emerging alternative is the estimated repetitions to failure (ERF) approach (e.g., terminating sets two repetitions from failure). Despite the importance of affective responses experienced during RT, a comparison between the two approaches on such outcomes is lacking.

Methods

Twenty women (age range: 23–45 years) without RT experience completed estimated one repetition maximum (RM) tests in four exercises. In the next two counterbalanced sessions, participants performed the exercises using 70%1RM. Participants completed ten repetitions in all three sets (predetermined condition) or terminated the sets when perceived to be two repetitions away from task-failure (ERF condition). Primary outcomes were affective-valence, enjoyment, and approach-preference and secondary outcomes were repetition-numbers completed in each exercise.

Results

We observed trivial differences in the subjective measures and an approximately even approach-preference split. Under the ERF condition, we observed greater variability in repetition-numbers between participants and across exercises. Specifically, the mean number of repetitions was slightly lower in the chest-press, knee-extension, and lat-pulldown (~1 repetition) but considerably higher in the leg-press (17 vs. 10, p<0.01).

Conclusions

Both approaches led to comparable affective responses and to an approximately even approach preference. Hence, prior to prescribing either approach, coaches should consider trainee’s preferences. Moreover, under the ERF condition participants completed a dissimilar number of repetitions across exercises while presumably reaching a similar proximity to task-failure. This finding suggests that ERF allows for better effort regulation between exercises.
Validation of a direct-to-PCR COVID-19 detection protocol utilizing mechanical homogenization: A model for reducing resources needed for accurate testing
Authors: Morehouse Zachary P. , Samikwa Lyson , Proctor Caleb M. , Meleke Harry , Kamdolozi Mercy , Ryan Gabriella L. , Chaima David , Ho Antonia , Nash Rodney J. , Nyirenda Tonney S.
Published: August 2021

Efficient and effective viral detection methodologies are a critical piece in the global response to COVID-19, with PCR-based nasopharyngeal and oropharyngeal swab testing serving as the current gold standard. With over 100 million confirmed cases globally, the supply chains supporting these PCR testing efforts are under a tremendous amount of stress, driving the need for innovative and accurate diagnostic solutions. Herein, the utility of a direct-to-PCR method of SARS-CoV-2 detection grounded in mechanical homogenization is examined for reducing resources needed for testing while maintaining a comparable sensitivity to the current gold standard workflow of nasopharyngeal and oropharyngeal swab testing. In a head-to-head comparison of 30 patient samples, this initial clinical validation study of the proposed homogenization-based workflow demonstrated significant agreeability with the current extraction-based method utilized while cutting the total resources needed in half.