bluuebunny/crossref_metadata_2025 huggingface dataset:
What happens to adiponectin, resistin and apelin-12 in colorectal adenomas? A cross sectional study.
Authors: dikbas oguz , Dagli Ülkü , Ankarali Handan , Tekce Buket Kin , Yılmaz Fahri , Gonen Mustafa Sait
Published: January 2020

BackgroundColorectal adenomas are precancerous neoplastic lesions which may potentially differentiate to the colorectal carcinoma. We investigated whether adiponectin, resistin and apelin 12 serum levels might change in case of colorectal neoplasia. Aims In this study we intended to determine relationship between serum levels of adiponectin, resistin, apelin-12 and presence of colorectal adenoma using case-control approach. Methods Patients undergoing screening colonoscopy in the Abant Izzet Baysal University Medical Faculty Gastroenterology Polyclinics between years 2010 and 2013 were selected for study. Results In this study there were not any difference between groups according to age, body mass index, waist circumference and mean arterial blood pressure (all p>0.05). Adiponectin, resistin and apelin-12 serum levels were not statistically different between groups (p=0.642, p=0.890, p=0.618; respectively). On the other side: Serum apelin-12 levels were found to be statistically higher in patients with severe dysplastic adenoma group compared to both non-dysplastic and without adenoma groups (p=0.014). There was a negative correlation between the number of colorectal adenomas and serum adiponectin levels (p=0.035, r=-0.41). Conclusion Apelin-12 does increase in severe dysplastic adenomas. Apelin-12 is an angiogenic adipocytokine with oncogenic potential. The relation between cancer development and apelin has been shown in different types of tumors. Apelin-12 might be a candidate marker for detecting dysplastic colorectal adenomas.
Screening of marine fungal strains for laccase-like activities amenable to biotechnological applications
Authors: ALI Wissal BEN , Chaduli Delphine , Navarro David , Lechat Christian , Turbé-Doan Annick , Bertrand Emmanuel , Faulds Craig B. , Sciara Giuliano , Lesage-Meessen Laurence , Record Eric , Mechichi Tahar
Published: January 2020

Background Environmental pollution is one of the major problems that world is facing to date. Several approaches are been studied and oxidative enzymes from microbial organisms represent an eco-friendly and cost–effective processes, amenable to biotechnological applications, as for instance industrial dye decolorization. The aim of this study was to screen marine-derived fungal strains isolated from three coastal areas in Tunisia, to identify laccase-like activities, and to produce and characterize active fungal secretomes of interest for dye decolorization. Results Following the screening of twenty fungal strains isolated from the harbours of Sfax and Monastir (Tunisia), five strains were identified displaying laccase-like activities. Molecular based taxonomic approaches allowed us to identify these strains as belonging to the species Trichoderma asperellum , Stemphylium lucomagnoense and Aspergillus nidulans . Among these five isolates, one T. asperellum strain ( T. asperellum 1) gave the highest level of secreted oxidative activities, and as such it was chosen for further studies. Optimization of the growth medium for liquid cultures was first studied to improve the level of laccase-like activity in culture supernatants. Finally, T. asperellum 1 secretome allowed decolorizing different synthetic dyes belonging to diverse dye families, in the presence or absence of 1-hydroxybenzotriazole (HBT) as a mediator. Conclusions The optimal growth conditions to produce laccase-like active secretomes from T. asperellum 1 were 1.8 mM CuSO 4 as an inducer, 1% NaCl to mimic seawater environment and 3% sucrose as a carbon source. T. asperellum 1 secretome was effective to decolorize different synthetic dyes belonging to diverse chemical classes, and the presence of HBT as a mediator improved the decolorization process.
Grape cultivar features differentiate the grape rhizosphere of microbiota
Authors: Yang Zhiguang , Sun Bo , Zhang Shiwei , Bao Lijun , Wei Yingxue , Li Zaixing , Bai Zhihui
Published: January 2020

Background: Despite its importance to crop health and plant quality, the structure and diversity of the grape rhizosphere remains poorly described. In this study, the microflora community structures of grapes in northern China were compared using high-throughput sequencing. Results: We found that richness, diversity of bacterial and fungi community networking in the root compartments were significantly influenced by the grape variety. The bacterial linear discriminant analysis showed that Pseudomonas , Rhizobium which considered as potential plant-growth-promoting bacteria were more enriched in Pinot noir, and potential ammonia-oxidizing Nitrosospira was enriched in Gem. The fungal linear discriminant analysis showed that Fusarium was more enriched in Longan, Sporormiella more enriched in Merlot while Gibberella and Pseudallescheria were more enriched in Gem, and Mortierella was more abundant in Cabernet Sauvignon. Conclusions: The 16S rRNA functional prediction indicated that no significance differentiate among the varieties. The results showed that may be we can extinguish the varieties according to these characteristic bacterial and fungal genus.
The exacerbation of soil acidification correlates with structural and functional evolution of the soil microbiome upon long-term agricultural intensification
Authors: Shen Jie , Luo Youlin , Li Meng , Tao Qi , White Philip J , Cadre Edith Le , He Yuting , Li Bing , Li Qiquan , Xu Qiang , Cai Yan , Li Huanxiu , Li Yiding , Tang Xiaoyan , Guo Lingke , Zhao Junwen , Wang Changquan
Published: January 2020

Background: Agricultural intensification induces prolonged impacts on soil acidification (SA), whereas linking the mechanisms therein to structural and functional evolution of soil microbiota remain unclear, especially over longer time scales. Methods: To investigate the short- and long-term effects of agricultural intensification on soil microbiome and its association with SA severity, we performed metagenomic sequencing of soil samples from typical rice-vegetable rotations for 0 (control, V0), 10 (V10), 20 (V20) years. The objectives were to clarify biomarkers that indicative of SA and its putative functions of acid production. Results: Rice-vegetable rotations for 0, 10, 20 years yielded three well-defined soil clusters with differential acid saturation (V0: 4.5%; V10: 11.8%; V20: 40.7%). Soil pH declined significantly (p < 0.05) from the 6.38 (V0) to 4.82 (V20). Acid cations (H + , Al 3+ ) dominating V20’s exchangeable cation pool, suggesting that soils in V20 were highly acidic and acid-sensitive, which recruited distinct microbiomes. The increased acid cations and NO 3 - -N concentrations are driving forces that lead to a higher abundance of Rhodanobacter , Gemmatirosa , Sphingomonas and Streptomyces in V20 samples, which acting as aciduric biomarkers that significantly and positively correlated with soil acidity. Furthermore, functional modules of microbial genes revealed V20 samples contained more genes associated with“oxidative phosphorylation” and “two-component system”, particularly enriched multiple pathways of cytoplasmic pH homeostasis. More specifically, we found increased genetic abundances of active efflux of protons and cytoplasmic proton consumption in V20 samples over than in V0, which are both involved in the microbial dehydrogenation and acid production, and favoured the growth of Rhodanbacter , Gemmatirosa , Sphingomonas , Streptomyces . Conclusions: Collectively, the identified aciduric biomarkers was closely associated with SA severity in intensive agroecosystems. An overall increase with the ongoing of intensive cropping history in the abundance of indicative genes associated with dehydrogenation and acid production pathways. The differential community compositions provide new clues to elucidate the interaction between the soil microbiome and SA severity, and varying dehydrogenation and acid production pathways provide novel insights into the exacerbation of SA. Keywords: Agricultural intensification, Soil acidification, Metagenomics, Soil microbiome, pH homeostasis
The cooperative complex of Argonaute-2/MicroRNA-146a regulates Hepatitis B Virus replication through FEN1
Authors: ji min , Mei Xiaoping , Jing Xunming , Xu Xu , Chen Xing , Pan Wanlong
Published: January 2020

Background: The regulatory of HBV replication is still unclear. FEN1 can repair HBV rcDNA to HBV cccDNA and promote HBV DNA replication. However, its specific regulatory detail remains unclear. MicroRNA regulates gene expression at post-transcriptional level. Especially, miR-146a, it plays an important role that is closely related to regulation of HBV replication. Based on above, we hypothesize that miR-146a may be regulate HBV cccDNA formation through FEN1. So, we will investigate the effect of miR-146a on the replication of hepatitis B virus and its molecular mechanism. Results: We found that level of miR-146a was significantly up-regulated in HepG2.2.15 cells (11.755±0.069) than that in HepG2 (1.000±0.038) (P<0.05). Furthermore, HBV-DNA copies and FEN1 were significantly increased and decreased, respectively, in HepG2.2.15 cells transfected with miR-146a mimic and inhibitor for 48h,[(3.215±0.001); (2.623±0.083)] compared with the control group (2.813±0.015) (P<0.05),. After transfection FEN1 plasmid, HBV-DNA Copies (5.712±0.371) is significantly higher than the control group（2.661±0.009）(P<0.05), and the level of miR-146a（3.431±0.004）is significantly higher than the control group (1.023±0.224） (P<0.05). The expression level of IRAK1/TRAF6 are significantly lower and higher [(0.114±0.013); (0.390±0.014); (1.222±0.073); (2.145±0.271)] than the control group [(1.000±0.038); (1.007±0.119)] (P<0.05) after transfection miR-146a mimic and inhibitor into HepG2.2.15. After Ago2 siRNA, the level of miR-146a (0.105±0.002) is significantly decreased than the control group (1.000±0.041) (P<0.05) from Ago2 protein RIP. After transfection Ago2 siRNA then added into exogenous miR-146a into HepG2.2.15, The expression level of FEN1 is significantly reduced (0.485±0.100) than the control group (1.000±0.023) (P<0.05), and the HBV-DNA copies is significantly lower (3.230±0.047) than the control group (3.789±0.041) (P<0.05). Conclusion: Ago2 cooperates with miR-146a to regulate the transcription the expression level of FEN1 protein through the downstream target gene IRAK1/TRAF6, then promoting HBV replication.
Red/ET recombineering enhances extracellular lipase production by Burkholderia glumae
Authors: Tu Qiang , Bian Zhilong , Fang Qian , Chen Hanna , Xie Zhiling , Pan Deng , You Beian , Xia Liqiu , Zhang Youming , Gong Yaling
Published: January 2020

Background: Microbial lipases are utilized widely in industrial fields, and the LipA lipase produced by Burkholderia glumae PG1 has been used for the production of enantiopure pharmaceuticals. However, efficient lipase expression and secretion are still problematic. LipA is encoded in an operon that also contains the lipase-specific foldase gene lipB. The purpose of this study was to enhance the production of active lipase by overexpressing lipAB in B. glumae PG1 using a host-vector system.Results: The lipAB operon, isolated from the genomic DNA of B. glumae PG1, was directly cloned into broad-host-range vectors, with pBBR1 or pRk2 backbones, using the Red/ET recombination system and then transformed into the original host strain. Lipase activity of the derivative strains, including PG1/Bl and PG1/Rl, were evaluated. When 1% olive oil was included in the growth medium, the maximum activities of the resultant strains were approximately 39.5- and 15.3-fold higher, respectively, than that of the wild-type PG1, indicating that increased lipase gene dosage resulted in high expression yields of lipase. To further enhance lipase expression, two recombinant strains, PG1/Ba and PG1/Ra, were generated with the plasmid-borne lipAB operon driven by the strong constitutive promoter Papra. PG1/Ba and PG1/Ra displayed 15.0- and 4.3-fold improvements in lipase production in 0.5% glucose-containing medium compared to PG1/Bl and PG1/Rl, respectively. However, glucose repressed lipase gene expression in strain PG1 when the native lipAB promoter was used.Conclusions: Homologous expression of lipase genes in B. glumae PG1 was achieved by the use of high-copy expression vectors, and the resulting presence of multiple copies of lipAB significantly improved extracellular lipase expression. Our results demonstrate that modification of B. glumae PG1 can improve this host system for lipase production.
Thermoelasticity of tremolite amphibole: Geophysical implications
Authors: Peng Ye , Mookherjee Mainak
Published: January 2020

We investigated the structure, equation of state, thermodynamics, and elastic properties of tremolite amphibole [Ca2Mg5Si8O22(OH)2] up to 10 GPa and 2000 K, using first principles simulations based on density functional perturbation theory. We found that at 300 K, the pressure-volume results can be adequately described by a third-order Birch-Murnaghan equation of state with bulk moduli K0 of 78.5 and 66.3 GPa based on local density approximation (LDA) and generalized gradient approximation (GGA), respectively. We also derived its coefficients of the elastic tensor based on LDA and GGA and found that the LDA result is in good agreement with the experimental results. At 300 K, the shear modulus G0 is 58.0 GPa based on LDA. The pressure derivative of the bulk modulus K′ is 5.9, while that of the shear modulus G′ is 1.3. The second Grüneisen parameter, or δT = [–1/(αKT)](∂KT/∂T)P, is 3.3 based on LDA. We found that at ambient conditions, tremolite is elastically anisotropic with the compressional wave velocity anisotropy AVP being 34.6% and the shear wave velocity anisotropy AVS being 27.5%. At higher pressure corresponding to the thermodynamic stability of tremolite, i.e., ~3 GPa, the AVP reduces to 29.5%, whereas AVS increases to 30.8%. To evaluate whether the presence of hydrous phases such as amphibole and phlogopite could account for the observed shear wave velocity (VS) anomaly at the mid-lithospheric discontinuity (MLD), we used the thermoelasticities of tremolite (as a proxy for other amphiboles), phlogopite, and major mantle minerals to construct synthetic velocity profiles. We noted that at depths corresponding to the mid-lithosphere, the presence of 25 vol% amphibole and 1 vol% phlogopite could account for a VS reduction of 2.3%. Thus based on our thermoelasticity results on tremolite amphibole, it seems that mantle metasomatism could partly explain the MLD.
Systematics of H2 and H2O evolved from chlorites during oxidative dehydrogenation
Authors: Lempart Małgorzata , Derkowski Arkadiusz , Strączek Tomasz , Kapusta Czesław
Published: January 2020

Thermally induced dehydroxylation and oxidative dehydrogenation drive the thermal decomposition of all Fe2+-containing phyllosilicates. Whereas the former produces H2O gas, the latter results in H2 evolution. Six chlorites representing the Mg-Fe2+ series from clinochlore to chamosite and biotite (as an analog of the 2:1 layer in chlorite) were investigated using thermogravimetry coupled to quadrupole mass spectrometry (TG-MS). A fast-ramp heating protocol was applied to identify if and how hydrogen gas was released from the crystal structure and whether it was quantitatively related to structural Fe2+ content. Dehydroxylation and oxidative dehydrogenation were tested under inert and oxidizing conditions.

H2 liberation confirmed the H2 gas production by oxidative dehydrogenation, as shown by an evolution of the m/z = 2 signal for chamosites, Fe-rich clinochlores, and biotite heated under nitrogen gas atmosphere. Along with H2 evolution, H2O (m/z = 18) was released, suggesting that dehydroxylation is a trigger for dehydrogenation. The higher the Fe2+ content in the studied chlorites, the more intense the H2 evolution, thus the higher the H2/H2O ratios. The products of ramp-heating to 1000 °C resulted in varying amounts of newly formed Fe3+ (from 7 to 22%), however, biotite that converted into oxybiotite underwent almost complete oxidation, indicating a stronger tendency of 2:1 layer to dehydrogenation. The observed concurrent, but independent mechanisms of H2 and H2O evolution produced a feasible model of the thermal decomposition of chlorites.

Despite O2 availability under oxidizing condition, the Fe2+ oxidation was not driven by attaching oxygen anions to the phyllosilicate structure, but also by dehydrogenation. Hydrogen was not detected using MS for any tested sample heated in synthetic air because any H2 if released was instantaneously combined with external O2, which resulted in an excess H2O MS signal not matched by mass loss on the TG profiles of chamosite and biotite. Without coupling of the evolved gas analysis with TG, the excess H2O produced by dehydrogenation in the O2-bearing carrier gas would result in misleading interpretations of dehydroxylation.

Methodological and geological implications of the TG-MS experiments are discussed. The oxidation of Fe2+ in all Fe2+-containing phyllosilicates proceeds with simultaneous H2 gas release that is not dependent on oxygen fugacity nor temperature during the mineral formation. Therefore, the correlation between Fe3+/Fe2+ and remaining hydrogen in the structure must be considered during modeling the conditions that involve chlorite as geothermobarometer. H2 release during heating is proposed as an indicator of oxidative dehydrogenation of Fe2+-bearing minerals on Mars.
INTEGRATING LEARNER CORPUS ANALYSIS INTO THE TEACHING OF ENGLISH ACADEMIC WRITING
Authors: Araminta Lavinia Disa Winona
Published: January 2020

Practical implementation of learner corpus research to inform language pedagogy has been common, given the availability of resources, such as a large amount of data about the products of language learning and factual language uses, and the necessary technology, such as concordance programs. This article lays out the typical analyses of learner corpora and the implications of and issues surrounding such studies on second/foreign language teaching based on the existing literature. More specifically, the article captures the need for a more extensive corpus of Indonesian learners’ English other than what is already available to represent more insights about English language teaching in Indonesia. Furthermore, it proposes the development of an in-house learner corpus for direct and indirect uses at Universitas Indonesia. An actual trial on building a sample learner corpus and running a lexical analysis demonstrates the plausibility of integrating learner corpus into the teaching of academic writing on higher-education levels.
Riemann surfaces for KPZ with periodic boundaries
Authors: Prolhac Sylvain
Published: January 2020

The Riemann surface for polylogarithms of half-integer index, which has the topology of an infinite dimensional hypercube, is studied in relation to one-dimensional KPZ universality in finite volume. Known exact results for fluctuations of the KPZ height with periodic boundaries are expressed in terms of meromorphic functions on this Riemann surface, summed over all the sheets of a covering map to an infinite cylinder. Connections to stationary large deviations, particle-hole excitations and KdV solitons are discussed.
Can model-based data products replace gauge data as input to the hydrological model?
Authors: Sivasubramaniam K. , Alfredsen K. , Rinde T. , Sæther B.
Published: January 2020

Hydrological models require accurate and representative meteorological inputs for better prediction of discharge and hence, the efficient management of water resources. Numerical weather prediction model-based reanalysis data products on the catchment scale are becoming available, and they could be an alternative input data for hydrological models. This study focuses on the applicability of a set of model-based data as input to hydrological models used in inflow predictions for operational hydropower production planning of three hydropower systems in middle Norway. First, the study compared the data products with gauge measurements. Then, Hydrologiska Byråns Vattenbalansavdelning (HBV) models of the three catchments were calibrated with three different meteorological datasets (model-based, gauge and observational gridded) separately using a Monte Carlo approach. It was found that the correlation between the model-based and gauged precipitation was highly variable among stations, and daily values showed a better correlation than hourly. The performance of model-based input data with daily timestep was nearly as good as the gauge or gridded data for the model calibration. Further, the annual simulated flow volume using the model-based data was satisfactory as similar to the gauge or gridded input data, which indicate that model-based data can be a potential data source for long-term operational hydropower production planning.
Energetic refurbishment of an existing large-scale MBR
Authors: Brepols C. , Drensla K. , Janot A. , Schäfer H.
Published: January 2020

Operations of an existing municipal MBR serving 80,000 inhabitants were improved energetically by implementing new automation routines for biology and membrane filtration. Finally, the MBR was refurbished by adding primary clarification and anaerobic sludge digestion to the treatment process, while specific energy consumption was reduced by one third to 0.65 kWh/m3 permeate produced. A combined heat and power engine generates electrical power on site from digester gas. The projected carbon footprint of plant operations will thus be halved by reducing indirect emissions from electricity consumption.
A Importância da Hospitalidade em Unidades de Saúde: Um Estudo De Caso No Hospital Municipal Ruth Cardoso De Balneário Camboriú/SC de Acordo com o Planetree – Filosofia Aplicada no Hospital Israelita Albert Einstein
Authors: Vieira Marina Tété
Published: January 2020

A hospitalidade exerce um diferencial na composição dos serviços prestados pelas unidades de saúde, garantindo aos usuários uma experiência diferenciada através das relações humanas. O objetivo desta pesquisa foi avaliar a hospitalidade no Hospital Municipal Ruth Cardoso, localizado em Balneário Camboriú/SC, de acordo com os parâmetros da filosofia Planetree. Este estudo de caso de caráter exploratório adotou como método a revisão bibliográfica, a análise documental, a coleta das informações através de questionários e a observação. A análise foi qualitativa com base nos referenciais adotados e na percepção das pesquisadoras, construída através do percurso metodológico. Como resultado, verificamos que o hospital não está de acordo com muitos dos parâmetros da filosofia Planetree.
O “DIA DO PATRIMÔNIO” EM PELOTAS/RS COMO UM ATO DE HOSPITALIDADE
Authors: Hallal Dalila Rosa , Müller Dalila , Chagas Pierre , Ramos Maria da Graça Gomes
Published: January 2020

Este artigo tem como objetivo problematizar o “Dia do Patrimônio” na cidade de Pelotas, Rio Grande do Sul, como um ato de hospitalidade, possibilitando a interação da comunidade com o seu patrimônio cultural material e imaterial. O “Dia do Patrimônio” é um evento anual que acontece na cidade desde o ano de 2013. É organizado pela Secretaria Municipal de Cultura (SECULT) e tem o propósito de aproximar a comunidade do patrimônio cultural da cidade. O trabalho configura-se como uma pesquisa qualitativa, baseada nas seguintes fontes: sites; revistas do Dia do Patrimônio elaboradas pela Secretaria Municipal de Cultura de Pelotas – SECULT em parceria com a Prefeitura Municipal de Pelotas; e duas entrevistas com colaboradoras da SECULT na época da criação do evento. Ressalta-se que o “Dia do Patrimônio” é um ato de hospitalidade, um evento que possibilita relações interpessoais, relações de convivência, sociabilidade e alteridade na cidade de Pelotas. O evento proporciona aos moradores e turistas descobrir, tomar consciência e valorizar as riquezas, sejam materiais ou imateriais, possibilitando repensar o patrimônio e sua diversidade na cidade de Pelotas
QUALIDADE DE VIDA NO TRABALHO: ESTUDO DE CASO EM UM HOTEL NA ORLA DE CABO BRANCO - JOÃO PESSOA – PB
Authors: Menezes Paula Dutra Leão de , Mello Ana Maria Nery Soares de , Felisberto Hanna Melissa Pereira , Nogueira Niedja Glória
Published: January 2020

A insatisfação e a desmotivação são problemas que afetam trabalhadores independentemente de sua posição na empresa hoteleira. Para lidar com esses problemas torna-se necessário encontrar elementos intrínsecos e extrínsecos ao ambiente de trabalho que afetam diretamente o trabalhador. Para tanto, é fundamental encontrar a origem da insatisfação e medi-la, para que o gestor tome as medidas acertadamente. Este artigo tem como objetivo analisar a percepção dos gestores em relação a Qualidade de Vida no Trabalho (QVT).  Este estudo de caso caracteriza-se por ser do tipo exploratório e qualitativo. O instrumento de coletas de dados foi um questionário fechado. A maioria dos aspectos avaliados apresentaram satisfação dos gestores. Entretanto, no aspecto satisfação sociológico/relacional sobre o quesito liberdade de expressão sem ser prejudicado quando expressa suas opiniões revelou-se insatisfatório, por sua vez, 75% dos gestores entrevistados sentem satisfação em se expressar. Sobre a satisfação psicológica/comportamental, o desenvolvimento pessoal e profissional também revela que os gestores estão insatisfeitos com o incentivo da empresa em termos de oferta de atividades e cursos relacionados a seu trabalho. Por último, registra-se a importância de observar a satisfação e motivação dos gestores considerando que são os líderes da equipe e consequentemente os motivadores dessas.
BENCHMARK BETWEEN SCIENTIFIC JOURNALS OF THE MANAGEMENT AREA
Authors: Cardoso André Luís Janzkovski , Silva Jennifer Nogueira Feitosa
Published: January 2020

The purpose of this research was to present the main distinguishing features among the 100 journals in the area of management available at the Scientific Journals Electronic Library (SPELL). The research has a quantitative and qualitative approach, having collected the data through a survey on the journals site, followed by a filtering in about 200 parameters, and the analysis of each periodical, based on Qualis/Capes 2013-2016 classification and with discriminant analyses. The results allowed to identify 25 distinctive characteristics that characterize each stratum Qualis/Capes and also provided a "map of steps" to follow to achieve each of the A2, B1, B2 and B3 classifications in the scientific editorial setting of the Management, Accounting and Tourism Areas. It should be emphasized that the journals of the different strata differ as to the aspects aimed at stimulating quality, techniques for attracting impact research and indexing mechanisms, elements that contribute to the development of the actors involved in the editorial process. To enrich the findings, it is suggested as future researches, besides applying the model presented here in the next classification Qualis/Capes 2017-2020, verifying the assertiveness of the discriminant analysis, and also applying the 25 variables presented in this study in a new research with international journals classified as A1 to identify the characteristics of this group, considering the lack of this stratum in national journals in the Management, Accounting and Tourism Areas.
Stakeholders Network: estudo das relações no Parque Ibirapuera de São Paulo
Authors: Enohi Nathalie Litsuko , Cintra Renato Fabiano , Costa Benny Kramer
Published: January 2020

O estudo analisou a estrutura da rede de stakeholders do Parque Ibirapuera. A investigação desenvolvida deriva da pouca literatura quanto ao estudo da estrutura, conteúdo e qualidade das interações entre os atores em redes de turismo, bem como trata-se do mais importante parque urbano de São Paulo/SP. Metodologicamente, a pesquisa caracteriza-se como qualitativa, exploratória e descritiva, desenvolvida a partir de estudo de caso único. As ferramentas utilizadas para expor as fontes de evidências se constituíram de levantamento bibliográfico, observação direta e entrevistas com 48 pessoas (3 do conselho gestor, 5 servidores terceirizados, 5 moradores locais, 5 guardas civis metropolitanos, 10 comerciantes autônomos e 20 frequentadores). A análise dos dados indicaram rede de densidade baixa, com apenas 3 stakeholders com alta centralidade (Conselho Gestor, Prefeitura de São Paulo e frequentadores). Os demais stakeholders foram categorizados de baixa centralidade. Como contribuição, o estudo tem caráter inovador decorrente da integração de três vertentes: a teoria dos stakeholders, análise de network e stakeholders network, bem como aplicação no turismo.
Conteúdos Gerados pelos Usuários sobre Meios de Hospedagem em Natal/RN: A Acessibilidade no discurso dos viajantes
Authors: Lamas Suellen Alice , Mendes Filho Luiz Augusto Machado , Perinotto André Riani Costa , Marques Júnior Sérgio
Published: January 2020

A partir dos anos 2000, com a criação de novas ferramentas tecnológicas, a comunicação social foi modificada. Nesta tendência, o turismo mudou para uma versão 2.0. Tal adoção deu voz aos turistas, visto que a Internet transmudou-se de uma plataforma publicitária para uma plataforma participativa e interativa, representada por aplicativos, serviços e comunidades virtuais. Neste cenário, os conteúdos gerados pelos usuários (CGU) começaram a receber maior atenção por parte dos gestores turísticos devido à sua influência nas decisões de compra e percepções de qualidade dos viajantes. Estudos quantitativos sobre determinantes, motivação e adoção desses conteúdos são comuns, mas quais temas são abordados nos mesmos? A Acessibilidade em meios de hospedagem, em voga no cenário nacional, compõe o discurso dos usuários? A partir dessas indagações, o presente artigo propôs verificar se (e como) o tema da Acessibilidade em meios de hospedagem de Natal, no Rio Grande do Norte, é retratado nos CGU do TripAdvisor. Para tanto, valeu-se de uma pesquisa exploratório-descritiva com abordagem qualitativa. Os resultados demonstraram que o discurso da Acessibilidade e seus termos correlatos perpassam pelos conteúdos dos CGU e são expressos por meio de críticas, elogios, sugestões e descrições, numa linguagem informal, mas consciente de uma realidade.
Aspectos morfoanatômicos de Senegalia angico (Mart.) Seigler & Ebinger. (Leguminosae): uma abordagem taxonômica
Authors: Zagotta Vital Flávio Antônio , Nakamura Adriana Tiemi , Terra Vanessa
Published: January 2020

Este trabalho descreve a morfoanatomia dos nectários extraflorais (NEF), dos tricomas, do pecíolo e da raque das folhas de Senegalia angico, visando encontrar características que auxiliem na sua delimitação dentro do “Complexo Tenuifolia”. Folhas frescas foram coletadas, fixadas, desidratadas, incluídas em historesina e seccionadas para a confecção de lâminas permanentes e semipermanentes. No pecíolo, a epiderme é unisseriada, com tricomas tectores, sendo de uni a multicelulares, e tricomas glandulares capitados localizados no sulco entre os feixes acessórios (FA). A região cortical é constituída de células de parênquima. O sistema vascular é composto por feixes acessórios e sistema vascular central (SVC). A vascularização do único NEF é realizada pelos traços emitidos do SVC. A raque apresenta anatomia semelhante ao pecíolo, porém com feixes acessórios em posição adaxial e periférica ao SVC, formando um sulco. Os sete NEF da raque são vascularizados pelos FA e pelo SVC. O último segmento da raque adquire secção transversal triangular, com apenas um FA. Os NEF do pecíolo e da raque são cupuliformes. Caracteres como tricomas glandulares, número, forma e vascularização dos nectários e a conformação triangular da porção final da raque são peculiares à espécie, sendo úteis para sua distinção dos integrantes do “Complexo Tenuifolia”, no entanto análises complementares são necessárias.
Application of response surface methodology and central composite rotatable design (CCDR) for modelling the influence of agro-industrial waste in lactic acid biosynthesis
Authors: Pezzin Ana Paula Testa , Capellari Jaqueline Boldt , Neves Eduarda Zeni , Garcia Michele Cristina Formolo , Apati Giannini Pasiznick , Schneider Andrea Lima Dos Santos
Published: January 2020

Lactic acid (LA) is one of the most important organic acids, with a wide range of industrial and biotechnological applications and can be produced by chemical synthesis and microbial culture. However, the biotech pathway is generally preferred because it provides an optically pure product. In this context, the purpose of this work was to evaluate LA biosynthesis by Lactobacillus amylovorus using molasses as carbon source (CS) and corn steep liquor as nitrogen source (NS) in a central composite rotatable design (CCRD) varying the concentration CS and NS, as well as to validate the model. The method for microbial culture followed an experimental design of the CCRD type, conducted without agitation, at 37ºC in Erlenmeyer flask, with pH in spontaneous evolution. The results showed that, using molasses and corn steep liquor as alternative sources, LA production ranged from 2.8 to 4.6 g/L, respectively, with the most favourable condition being 40.0 g of molasses and 250 g of corn steep liquor. It was possible, from the experimental design, to ascertain the selection of the best conditions for the microbial culture, demonstrating the feasibility of replacing CS and NS by agro-industrial waste, thus reducing the cost of producing LA.
Novas interpretações do patrimônio cultural edificado em tempos hipermodernos em Caxias do Sul (Brasil) e La Plata (Argentina)
Authors: Caon Marcelo
Published: January 2020

Este artigo propõe um olhar sobre a constituição do campo relativo à preservação do patrimônio histórico, bem como a sua instrumentalização pelo Estado ou por grupos preservacionistas sem poder legislador. Busca-se desvelar a reorientação preservacionista como forma de resistência às transformações da hipermodernidade ou à acomodação ao seu efeito. Da nacionalização patrimonial do Brasil e da Argentina, como ocorreu na resistência causada pela pluralidade das memórias coletivas no fim do século XX, estruturaram-se maneiras de contrastar a fragmentação das cidades e de seus modelos de gestão, que privilegiam a funcionalidade arquitetônica e a especulação imobiliária. Deu-se daí, no campo conceitual, o surgimento dos grupos que estabeleceriam ações preservacionistas atentas à incorporação de novos elementos de ressonância, reflexo da descentração do sujeito hipermoderno. Isso se fez possível por intermédio de ferramentas capitais, como o inventário patrimonial, que confere estatuto para o debate sobre a preservação e serve aqui como fonte para o desenvolvimento de um estudo de caso comparado das cidades de Caxias do Sul (Brasil) e La Plata (Argentina).
Ação de educação patrimonial na comunidade de Campo Bom, município de Jaguaruna – SC
Authors: Zamparetti Bruna Cataneo , Pinheiro Micaella Schmitz , Luiz Carolina Porto
Published: January 2020

Grupo de Pesquisa em Educação Patrimonial e Arqueologia (Grupep) da Universidade do Sul de Santa Catarina (Unisul) atua há 19 anos no desenvolvimento de pesquisas e na difusão do patrimônio arqueológico, com enfoque no patrimônio catarinense. Ao longo desses anos o grupo desenvolveu inúmeras ações educativas, entre as quais propomos apresentar neste relato as realizadas no âmbito do Programa de Salvamento Arqueológico e Educação Patrimonial na área de influência indireta do Aeroporto Regional Sul Humberto Ghizzo Bortoluzzi, município de Jaguaruna (SC), no ano de 2018. As atividades foram desenvolvidas com estudantes de 6.º ano do ensino fundamental da EEB Campos Verdes, no município de Jaguaruna, sul de Santa Catarina. Utilizaram-se metodologias ludopedagógicas de ensino aliadas a saída de campo durante escavação arqueológica. Todas as ações desenvolvidas se mostraram relevantes para aproximar os estudantes do patrimônio arqueológico regional, principalmente aquele que se encontra muito perto da instituição escolar, auxiliando na formação de um sentimento de valorização e subsequente preservação de tais espaços.
Narrativas literárias e referências de tempo e espaço em paisagens representativas do imaginário coletivo
Authors: Klein Regina Maria Martins de Araujo , Hardt Letícia Peret Antunes , Hardt Carlos
Published: January 2020

Diante da interpretação da cidade como imagem de ações da sociedadee considerando a necessidade de compreensão do imaginário humano para aconstrução de identidades individuais e grupais, o objetivo geral desta pesquisaconsiste em analisar o contexto em que a paisagem urbana tem sido referenciada no tempo e no espaço pela memória coletiva, adotando narrativas literárias de Manhattan (Nova York, Estados Unidos) como estudo de caso. Com base em postulados teóricos sobre os temas centrais de investigação, os procedimentos metodológicos foram fundamentados em um recorte amostral de 99 narrações de publicações no intervalo temporal de 196 anos, sistematizadas pela sua localização em mapa específico daquele distrito administrativo para identificação da frequência de citação por tipologia de elemento urbanístico (caminhos, limites, regiões, pontos nodais e marcos) e de ordenamento no tempo (horizontal: continuidade; vertical: especificidade). A análise dos resultados aponta, de forma intrínseca, para a legibilidade da regiãoestudada, com especial destaque para vias públicas e, de maneira genérica, para a necessidade de aprofundamento dos estudos para a devida estruturação de subsídios aos processos de planejamento de paisagens urbanas.
An observational study of dental abnormalities in the primary teeth
Authors: Hartwig Andréia Drawanz , Azevedo Marina Sousa , Pauli Laís Anschau , Jahnecke Katerine Arteiro , Pappen Fernanda Gerardo , Romano Ana Regina
Published: January 2020

Introduction: Pregnancy and early childhood are the most critical phases with regard to biological, cognitive, emotional, and social development. Adverse events in these periods of life may be related to alterations in dental development, including alterations in size, shape and mineralization. Objective: To evaluate the frequency of dental abnormalities and the factors associated with the developmental defects of the enamel in primary teeth. Material and methods: Information about family income, maternal education level, pretermbirth, and hospitalization history up to 11 months of age were collected from the dental records of 544 children. Clinical examination of the children was performed to investigate abnormalities of tooth number, form, size, and developmental defects of the enamel (DDE). Data were analyzed using χ2 test and Poisson regression. Results: In all, 544 children were evaluated. Sixty children (11.0%) presented some alterations in the primary teeth; and 7.5% showed DDE. Children born preterm were 3.17 times more likely to develop DDE in primary teeth (prevalence ratio – PR = 3.17, 95% confidence interval– 95%CI 1.26–7.98, p = 0.014). Among the alterations of number, 1.7% was hypodontia, and among the abnormalities of shape, 1.7% was fused teeth. Conclusion: The prevalence of dental anomalies in this sample was, in general, expressive. Children born preterm were more vulnerable to present developmental defects of the enamel.
Associação de técnicas cirúrgicas para o tratamento da rânula na cavidade bucal Association of surgical techniques for the treatment of ranula in the oral cavity
Authors: Lago Eliana Campêlo , Faria Natália Spadini de , Marcaccini Andrea Marcia , Ribeiro Ivonizete Pires , Almeida Camila Aparecida Pinheiro Landim , Batista Moura Luana Kelle
Published: January 2020

Introdução: Rânula é uma lesão que acomete glândula salivar menor, especialmente no assoalho bucal, resultando na obstrução parcial ou completa do ducto excretor glandular, com retenção da secreção. O tratamento dessa lesão inclui micromarsupialização e remoção cirúrgica por diferentes técnicas. Objetivo: Apresentar um estudo de caso sobre a técnica cirúrgica de drenagem associada à excisão cirúrgica no tratamento da rânula em paciente pediátrico.Relato de caso: Paciente do sexo masculino, 13 anos, apresentou cisto de retenção salivar e foi atendido em clínica-escola privada em Teresina (PI). Após o exame clínico e a formulação da hipótese de diagnóstico, o tratamento foi definido como aspiração e remoção. A lesão que se apresentava mais distendida foi tratada por meio de drenagem por micromarsupialização e posteriormente removida por excisão cirúrgica, tornando possível menor envolvimento de estruturas adjacentes. Conclusão: O diagnóstico clínico da rânula possibilita o tratamento mais adequado, com preservação de tecido impedindo a recidiva da lesão, entretanto deve-se sempre ter como auxílio o exame histopatológico
Characterization of the Active Components of the Multimerized sTNFRII-Adiponectin Fusion Protein Showing Both TNFα-Antagonizing and Glucose Uptake-Promoting Activities
Authors: Wang Yao , Lian Hui , Wang Xitong , Zheng Tianyu , Yu Xiaoxiao , Chen Ruzhang , Huang Zhiyong , Lv Yinxiang , Zhao Ai , Gao Jimin
Published: January 2020

Background:

The sTNFRII-adiponectin fusion protein previously showed strong TNFα
antagonistic activity. However, the fusion protein exists as mixture of different multimers. The aim of
the present study was to characterize its active components.

Methods:

In this study, the fusion protein was isolated and purified by Ni-NTA affinity and gel exclusion
chromatography, and further identified by Coomassie staining and western blotting. The TNFα
antagonistic and glucose uptake-promoting activities were determined in vitro. The glucose detection
kit and 2- NBDG (2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-glucose) were used to
measure their effects on glucose metabolism (including glucose consumption and glucose uptake in
HepG2 and H9C2 cells). The effect of the fusion protein on glucose uptake was also examined in free
fatty acid (FFA)- induced insulin resistance cell model.

Results:

The sTNFRII-adiponectin fusion protein was found to exist in three forms: 250 kDa (hexamer),
130 kDa (trimer), and 60 kDa (monomer), with the final purity of 90.2%, 60.1%, and 81.6%,
respectively. The fusion protein could effectively antagonize the killing effect of TNFα in L929 cells,
and the multimer was found to be superior to the monomer. In addition, the fusion protein could increase
glucose consumption without impacting the number of cells (HepG2, H9C2 cells) in a dosedependent
manner. Mechanistically, glucose uptake was found to be enhanced by the translocation of
GLUT4. However, it could not improve glucose uptake in the cell model of insulin resistance.

Conclusion:

In summary, the active components of the fusion protein are hexamers and trimers. The
hexamer and trimer of sTNFRII-adiponectin fusion protein had both TNFα-antagonizing and glucose
uptake-promoting activities, although neither of them could improve glucose uptake in the cell model
of insulin resistance.
Molecular Modelling, Synthesis and Biological Evaluation of Novel Benzimidazole Derivatives for the Treatment of Breast Cancer
Authors: Panchal Ishan , Devgirkar Animesh G. , Patel Ashish D. , Nagani Afzal , Lad Chaitali
Published: January 2020

Background:

The treatment of cancer requires scientific advancement. ATP-competitive mTOR
inhibitors have been studied as potential antitumor agents.

Objective:

A series of substituted benzimidazole compounds were designed, synthesized and characterized via
introducing 2-chloroquinolin into 2nd position and most title compounds exhibited enhanced anticancer activities.

Methods:

To study the anticancer mechanism, VIa-VIh was successfully docked by iGEMDOCK 2.0
which gives good affinity towards m-TOR/PI3K dual inhibitors. The anti-proliferative activities of these
compounds were evaluated on MCF-7 and A549 cell line for Breast and lung cancer, respectively.

Results:

2-(2-chloroquinolin-3-yl)-1H-benzoimidazol-1-yl)(phenyl)methanone (VIa) exhibited significant
anti-proliferative activity, especially against breast cancer (IC50 197 μM) for MCF7 cell line and (2-(2-
chloroquinolin-3-yl)-1H-benzo[d]imidazol-1-yl)(4-nitrophenyl)methanone (VIc) was significantly active
against lung cancer (IC50 89 μM) for A579 cell line.

Conclusion:

VIa gives more activity on breast cancer and it gives IC50 197 μM for MCF7 cell line and (2-
(2-chloroquinolin-3-yl)-1H-benzo[d]imidazol-1-yl) (4-nitrophenyl) methanone (VIc) lung cancer IC50 89
μM for A579 cell line.
COMPARISON OF NATIONAL EARLY WARNING SCORE (NEWS) AND REVISED TRAUMA SCORE (RTS) IN THE OUTCOME PREDICTION OF HEAD INJURY PATIENTS
Authors: Mapagresuka Iseu , Wahid Abdurahman , Hafifah Ifa
Published: January 2020

Head injury is a global problem with mortality and morbidity outcome of 90%. One of the factors that infuence the outcome is the physiological status that can be assessed by using NEWS and RTS as simple validated assessments to predict outcome. This study aimed to compare NEWS and RTS in the outcome prediction of head injury patients. This study used an analytic observational method with prospective cohort design and consecutive sampling technique type in 48 head injured patients at Emergency Room of Ulin Banjarmasin Hospital, Idaman Banjarbaru Hospital, and Ratu Zalecha Martapura Hospital conducted from November 2017 to January 2018. Based on this study, the average age of head injury patients was 35.54 years, male, caused by traffic accidents, and 87.5% of people had living outcomes. Statistical analysis with a diagnostic test revealedthat NEWS accuracy in the outcome prediction of head injury patients had AUC value of 0.911, p<0.001 with 83.33% sensitivity and 83.33% specificity. RTS accuracy analysis in the outcome prediction of head injury patients had AUC value of 0.879, p = 0,001 with 83,33% sensitivity and 92,86%specificity. RTS was excelled and more able to predict the outcome of head injury patients compared to NEWS.
Euanthes and the World of Rhetoric in Achilles Tatius’ Leucippe and Cleitophon
Authors: McHugh Katherine A.
Published: January 2020

Within Leucippe and Cleitophon, Achilles Tatius inserts three extremely detailed ekphraseis of paintings, all of which stand out amongst the many other descriptive passages in the novel. This paper explores the rhetorical background of the author’s use of ekphrasis, and focuses in particular on the artist ‘Euanthes’ who is named at 3,6,3 as the painter of the images of Andromeda and Prometheus. It seeks to prove that Euanthes is entirely a construction of the author and that the name is representative of the world of rhetoric prominent in much of the literature of the 2nd Century AD. The rhetorical nature of the other ekphraseis of paintings in Leucippe and Cleitophon is also explored in order to support the interpretation of Euanthes as being part of an author’s in-joke with his educated readers.