bluuebunny/crossref_metadata_2025 huggingface dataset:
Nickel Effects on Growth and Phytolith Yield of Grasses in Contaminated Soils
Authors: Silva Enilson de Barros , Farnezi Múcio Mágno de Melo , Santos Lauana Lopes dos , Silva Alexandre Chistofaro , Grazziotti Paulo Henrique , Alleoni Luís Reynaldo Ferracciú , Horák-Terra Ingrid , Nascimento Sandra Antunes do , Uane Bento Gil
Published: January 2024

Nickel (Ni) is extremely toxic to plants at high concentrations. Phytoliths have the potential to sequester the heavy metals absorbed by plants and act as a detoxification mechanism for the plant. The authors of the present study aimed to evaluate the effects of Ni on the growth and phytolith yield of grasses in two artificially contaminated soils. Two experiments separated by soil types (Typic Quartzipsamment and Rhodic Hapludox) were conducted in a completely randomized design in a 2 × 4 factorial scheme with three replications. The factors were two species of grass (Urochloa decumbens and Megathyrsus maximus) and three concentrations of Ni (20, 40, and 120 mg kg−1) and control treatment. The grasses were influenced by the increase in Ni rates in the soils. Ni exerted a micronutrient function with the addition of 30 mg kg−1 of Ni in soils, but this concentration caused toxicity in grasses. Such a level is lower than the limits imposed by the Brazilian environmental legislation. Higher Ni availability in Typic Quartzipsamment promoted Ni toxicity, with reduced growth and increased phytolith yield in the shoot, increased Ni in the shoot, and Ni occlusion in phytoliths by grasses, in comparison with Rhodic Hapludox. The yield and Ni capture in phytoliths by grasses in Ni-contaminated soils are related to the genetic and physiological differences between grasses and Ni availability in soils. Ni capture by phytoliths indicates that it may be one of the detoxification mechanisms of Urochloa decumbens to Ni contamination, providing additional tolerance. Megathyrsus maximus may be a future grass for the phytoremediation technique in Ni-contaminated soils.
Phytochemical Characterization of Callistemon lanceolatus Leaf Essential Oils and Their Application as Sustainable Stored Grain Protectants against Major Storage Insect Pests
Authors: Ankitha Thachappilly A. , Visakh Naduvilthara U. , Pathrose Berin , Mori Nicola , Baeshen Rowida S. , Shawer Rady
Published: January 2024

Food storage has been important since the dawn of agriculture and human settlement. Insect pests cause major losses to food grains during storage and production. Essential oils are good alternatives for chemical insecticides for the management of storage pests. Red bottlebrush, Callistemon lanceolatus, is a flowering plant of the Myrtaceae family. This research work aimed to extract the oil from bottlebrush leaves, and chemically characterize and assess their repellent and insecticidal properties against the cowpea seed beetle, Callasobruchus maculatus (F.) (Coleoptera: Chrysomelidae), cigarette beetle, Lasioderma serricorne (F.) (Coleoptera: Ptinidae), and red flour beetle, Tribolium castaneum (Herbst.) (Coleoptera: Tenebrionidae), for the first time. The essential oil yielded by hydro-distillation of bottlebrush leaves was 1.02 ± 0.01%. GC-MS analysis determined the chemical composition of the volatile oil comprised 1,8-cineole (19.17%), α-terpineol (11.51%), α-pinene (10.28%), and α-Phellandrene (9.55%). The C. lanceolatus leaf oil showed potent repellence, contact toxicity, and fumigation toxic effects. In the contact toxicity assay, at 24 h, the LC50 values were 1.35, 0.52, and 0.58 mg/cm2 for the red flour beetle, cigarette beetle, and cowpea seed beetle, respectively. Likewise, in the fumigation assay observed after 24 h, LC50 values of 22.60, 5.48, and 1.43 µL/L air were demonstrated for the red flour beetle, cigarette beetle, and cowpea seed beetle, respectively. Additionally, there was no significance found by a phytotoxicity assay when the paddy seeds were exposed to C. lanceolatus oil. The results show that the volatile oils from red bottlebrush leaves have the potential to be applied as a biopesticide. Therefore, C. lanceolatus leaf oil can be utilized as a bio-insecticide to control stored product insects.
The Impact of Environmental Regulations on Carbon Emissions of Chinese Enterprises and Their Resource Heterogeneity
Authors: Pan Tuan , Zhang Juan , Wang Yan , Shang Yuping
Published: January 2024

At present, net-zero emissions have become a widely accepted goal globally. For a giant carbon emitter like China, especially after just experiencing a high-energy consumption, high-emission, and low-efficiency extensive economic model, achieving the global net-zero emissions target by the middle of this century is particularly important. The implementation of environmental regulation policies is one of the inevitable choices for achieving carbon peak and carbon neutrality. Existing theoretical analysis shows that environmental regulation acts on pollution emissions through cost effects and technological innovation, but relevant studies mostly focus on macro effects and ignore the impact of enterprise heterogeneity. This study calculates the carbon emission data of listed enterprises in China from 2012 to 2021 and examines the impact of environmental regulation policies on the carbon emission intensity of enterprises and its transmission mechanism from both theoretical and empirical perspectives. At the same time, the heterogeneity effect of resource-based industry and non-resource-based industry is considered. The research results show that China’s environmental supervision has been increasing year by year, which can reduce the carbon emission intensity of enterprises by improving the level of environmental disclosure of enterprises, environmental management concepts, and resource allocation efficiency and accelerating the establishment of environmental systems of enterprises, but the effect of technological innovation has not been highlighted. Further heterogeneity also indicates that environmental regulation is more conducive to reducing the carbon emission intensity of non-resource-based enterprises, small enterprises, and non-state-owned enterprises. The conclusions of this paper provide a precise direction for the implementation of environmental regulation policies in China and the world.
The Spatiotemporal Analysis of Land Take Exemplified by Poland
Authors: Elzbieta Bielecka
Published: January 2024

The research was motivated by the growing interest of scientists and practitioners in land consumption. It was assumed that the multifaceted and space–time analysis of the dynamics of land use change reveals agricultural and forest land conversion into artificial areas, and thus highlight the regions of high human pressure. To fulfill the research objective, the proprietary coefficient of admissible (maximal) land take (aLT) was used. This study, based on open, publicly available spatial and statistical data, presents agricultural and forest land losses in four periods (2005, 2010, 2015, 2020) in Polish provinces. The analysis reveals both the value and the trend of land take and indicates Mazowieckie and Małopolska as the provinces of the highest land take pace since 2005. In contrast, provinces such as Zachodnio-Pomorskie and Opolskie, located in the northwest and southwest of Poland, are characterized by small and decreasing losses of agricultural and forest land, prompting them to be classified as lower outliers. The paper concludes, in part, that admissible (maximal) land take (aLT) is a useful tool for monitoring land conversion and planning spatial development of any region in the world.
Navigating Time: A Comparative Analysis of Senior Tourists’ Intentions and Length of Stay in Italy Pre-, during, and Post-COVID-19
Authors: Campolo Maria Gabriella , Giannetto Carlo , Lanfranchi Maurizio , De Pascale Angelina
Published: January 2024

The worldwide COVID-19 pandemic led to a rapid global health crisis, which shocked the tourism industry by closing borders and reducing tourism demand. This study aims to analyze the impact that the COVID-19 pandemic has had on senior Italian tourism. If, on the one hand, seniors represent, from a tourism perspective, one of the most promising markets for the near future; on the other hand, they are the population that has most suffered the consequences of the pandemic event. Drawing on national statistical data, this study examines the impact of COVID-19 on the holiday intentions and length of stay of senior Italian tourists in Italy. The proposed methodological approach evaluates both travel intentions and length of stay by comparing the pre-pandemic, pandemic, and post-pandemic periods. It breaks down the decision-making process into two phases (to go on holiday or not, and length of stay) and is based on the estimation of a probit model with sample selection, which provides a more accurate approach to addressing the complex decision-making dynamics surrounding travel during pandemic periods, offering greater precision in modeling the factors influencing travel intention and length of stay. The results show a strong correlation between the decision to travel and the length of stay in the period before the pandemic. However, during the post-pandemic period there is a change in dynamics, where other factors gain importance in determining the length of stay regardless of the decision to travel.
Novel Ultra-High-Performance Concrete (UHPC) Enhanced by Superhydrophobic and Self-Luminescent Features
Authors: Mumtaz Ahmad Rizwan , Bede Odorčić Natalija , Garro Núria , Lubej Samo , Ivanič Andrej , Comite Antonio , Pagliero Marcello , Kravanja Gregor
Published: January 2024

This study explores the potential of using basalt reinforced UHPC by incorporating simultaneously self-cleaning and self-luminescent features, paving the way for sustainable advancements in civil engineering. New green formulations of UHPC were developed by integrating supplementary cementitious materials and optimizing water to the binder ratio, followed by using basalt fibers to enhance strength and ductility. The fabricated samples with high particle-packing density exhibit sufficient workability and compressive strength up to 136 MPa, and, when incorporating basalt fibers, a notable reduction in brittleness. The inner microstructure of basalt fibers was observed to be smooth, homogeneously distributed, and well adhered to the UHPC matrix. To ensure the desired long-lasting visual appearance of decorative UHPC and reduce future maintenance costs, a time-effective strategy for creating a light-emitting biomimetic surface design was introduced. The samples exhibit high surface roughness, characterized by micro to nano-scale voids, displaying superhydrophobicity with contact angles reaching up to 155.45°. This is accompanied by roll-off angles decreasing to 7.1°, highlighting their self-cleaning features. The self-luminescence feature showcased intense initial light emission, offering a potential energy-efficient nighttime lighting solution.
Amphibious Architecture: A Biomimetic Design Approach to Flood Resilience
Authors: Ameh Hope , Badarnah Lidia , Lamond Jessica
Published: January 2024

Amphibious buildings use the buoyancy principle in the design of their foundation systems to mitigate flood impact. In some cases, amphibious buildings are fitted with mechanical systems that further aid the buoyancy element to temporarily raise the building and guide its descent to natural ground level. These mechanical systems require external operation, preventing the amphibious building from passively responding during flood events as is one of the requirements of a robust flood mitigation measure. Additionally, buildings in flood environments are often left with stains on the exterior facade from floodwater contamination from sewage and chemicals, among others. This paper distinguishes three main components of an amphibious foundation: the buoyancy element, vertical guidance post, and structural sub-frame, and discusses their functionality. The natural world provides solutions to tackling environmental issues such as flooding. When systematically studied and transferred, nature can inspire innovative ideas for functional and sustainable designs for the built environments. Although there are many existing designs and a small number of constructed amphibious buildings, there are very few studies that discuss how the designs are derived, and even fewer on a framework emulating natural systems for transfer into amphibious building design. In that context, this research uses the biomimetic transfer process to abstract relevant biological systems, illustrating their potential for transfer into amphibious foundation design. The aim is to understand how these biological systems passively and continuously respond and adapt to their environment. Organisms such as the Venus flower basket, giant kelp, and red mangrove, among others, are discussed, to understand how they perform the identified functions. The steps of the biomimetic transfer process are used to integrate functions of amphibious buildings and processes of the studied biological systems. The final output of this paper is a discussion of the ways in which these derived relationships can be adopted in amphibious building design.
Future Projections of Heat Waves and Associated Mortality Risk in a Coastal Mediterranean City
Authors: Papadopoulos Giorgos , Keppas Stavros C. , Parliari Daphne , Kontos Serafim , Papadogiannaki Sofia , Melas Dimitrios
Published: January 2024

Climate change has been linked to the escalating frequency, duration, and intensity of heat waves in the Mediterranean region, intensifying health concerns for the general populace. Urban environments face elevated health risks due to concentrated populations and the urban heat island effect, further amplifying nighttime heat conditions. This study aims to project changes in heat wave characteristics and the associated population exposure risk in a large Mediterranean city, Thessaloniki, Greece. High-resolution climate simulations, using the WRF model, were conducted for three 5-year periods (2006–2010, 2046–2050, 2096–2100) under the RCP8.5 emission scenario, covering Thessaloniki with a 2 km grid. By the end of the century, Thessaloniki is projected to experience over 60 annual heat wave days, compared to ~8 in the present climate, while some episodes were found to persist beyond 30 days. The relative risk during heat wave days is expected to rise, which is primarily due to nighttime heat stress. Interestingly, the results indicate that minimum apparent temperature might be a more reliable indicator in predicting heat-related mortality compared to maximum apparent temperature. These findings emphasize the growing importance of informed heat mitigation and adaptation strategies and healthcare preparedness in urban areas facing escalating heat-related health challenges.
Simulation Study on Water Quality of Paddy Field Ditches Considering the Effects of Rainfall and Sediment Release
Authors: Shi Lujian , Li Songmin
Published: January 2024

As the main channel for the drainage of paddy fields, the water quality of canals directly affects the water quality of surrounding water bodies. Factors such as rainfall and the release of accumulated pollutants from the sediment of the canals have a direct impact on the water quality of drainage ditches. Based on the measured hydrological and water quality data, a dynamic water quality model was built to simulate the runoff and water quality changes in a paddy field for different return periods (1, 2, 5, 10, 20, and 50 years), and to consider the effects of released drainage sediment on the water quality of the paddy field. The change in water quality in paddy fields and ditches in different periods was studied. The simulation results showed that under different return periods, the total nitrogen concentration and total phosphorus concentration in the water of the paddy field and ditch increased first, and then decreased with time in June and July–September, while the pollutant concentration remained basically stable after the end of rainfall. With the increase in return period, the total nitrogen concentration and total phosphorus concentration decreased gradually. The release of nitrogen and phosphorus from the sediment of ditches resulted in an increase in the total nitrogen concentration and total phosphorus concentration in the ditches, and an increase in the pollution load. Under the influence of rainfall and fertilizer, the total nitrogen concentration and total phosphorus concentration in paddy ditch drainage were generally higher and more polluted in June than in July–September, and under the influence of released sediment, the ditch drainage was most polluted in June when the return period was one year, with the total nitrogen concentration and total phosphorus concentration at the outlet of the paddy ditch reaching 21.63 mg/L and 0.88 mg/L, respectively. The research results can provide a theoretical basis and basic support for the interception and treatment of non-point source pollution in farmland.
Agricultural Bioinputs Obtained by Solid-State Fermentation: From Production in Biorefineries to Sustainable Agriculture
Authors: Rocha Thiago Moura , Marcelino Paulo Ricardo Franco , Da Costa Rogger Alessandro Mata , Rubio-Ribeaux Daylin , Barbosa Fernanda Gonçalves , da Silva Silvio Silvério
Published: January 2024

Agriculture plays a major role on society, especially in developing countries which rely on commodity exportation markets. To maintain high crop productivity, the use of agrochemicals was once employed as the main strategy, which in turn affected soil, water, and human health. In order to aid this issue, identifying some alternatives, such as the implementation of biofertilizers and inoculants as bioinputs in modern agriculture, are imperative to improve ecosystem quality. Among these bioinputs, a few bioproducts have shown good performances, such as phytohormones (e.g., auxins and giberellins), biosurfactants, and other enzymes; thus, it is extremely important to assure the quality and feasibility of their production in biorefinery scenarios. These bioproducts can be synthesized through fermentation processes through utilizing plant biomasses and agricultural byproducts as carbon sources. In this sense, to increase the tecno-economical availability of these processes, the implementation of solid-state fermentation (SSF) has shown great potential due to its ease of operation and cost-attractiveness. Therefore, this study aims to describe the main substrates used in SSF systems for the production of potential bioinputs; their associated operation hurdles, parameters, and conditions selection; the most suitable microorganisms; and the underlying mechanisms of these molecules in soil dynamics. Within this context, this study is expected to contribute to the development of new processes in modern biorefineries and to the mitigation of environmental impacts.
Nature-Based Interventions for Adults with Developmental Disabilities: A Scoping Review Centering Autistic Adults
Authors: Dennis Megan , Henderson-Wilson Claire , Watson Joanne , Lawson Justin T.
Published: January 2024

Autistic adults often face higher levels of depression, anxiety, and stress and reduced levels of quality of life and employment in comparison to their neurotypical peers. Nature-based interventions (NBIs), which utilize outdoor environments and activities for health and wellbeing outcomes, could provide possibilities to meet these various needs. This study therefore aims to identify the extent of academic literature examining NBIs for autistic adults. A scoping review of peer-reviewed articles published between 1985 and 2023 was conducted. Ten databases were systematically searched, and additional hand searching was conducted. Studies involving substantial engagement with an NBI and involving autistic adults (age ≥ 18), or developmental disability if specific diagnosis was not identified, were included. A total of seven studies were identified; however, three studies did not refer to autism specifically, identifying developmental disability more broadly. NBIs were conducted in a variety of international locations and involved a diverse range of activities and outcomes. While NBIs can provide outcomes relevant to employment, health, and wellbeing, multiple limitations and literature gaps are noted. The findings indicate a strong need for further research specific to autism.
Real Estate Industry Sustainable Solution (Environmental, Social, and Governance) Significance Assessment—AI-Powered Algorithm Implementation
Authors: Walacik Marek , Chmielewska Aneta
Published: January 2024

As the global imperative for sustainable development intensifies, the real estate industry stands at the intersection of environmental responsibility and economic viability. This paper presents a comprehensive exploration of the significance of sustainable solutions within the real estate sector, employing advanced artificial intelligence (AI) algorithms to assess their impact. This study focuses on the integration of AI-powered tools in a decision-making process analysis. The research methodology involves the development and implementation of AI algorithms capable of analyzing vast datasets related to real estate attributes. By leveraging machine learning techniques, the algorithm assesses the significance of energy efficiency solutions along with other intrinsic and extrinsic attributes. This paper examines the effectiveness of these solutions in relation to the influence on property prices with a framework based on an AI-driven algorithm. The findings aim to inform real estate professionals and investors about the tangible advantages of integrating AI technologies into sustainable solutions, promoting a more informed and responsible approach to industry practices. This research contributes to the growing interest in the connection of the real estate sector, sustainability, and AI, offering insights that can guide strategic decision making. By implementing the random forest method in the real estate feature significance assessment original methodology, it has been shown that AI-powered algorithms can be a useful tool from the perspective of real estate price prediction. The methodology’s ability to handle non-linear relationships and provide insights into feature importance proved advantageous in comparison to the multiple regression analysis.
Organizational Risk Prioritization Using DEMATEL and AHP towards Sustainability
Authors: Yazo-Cabuya Eliana Judith , Herrera-Cuartas Jorge A. , Ibeas Asier
Published: January 2024

Risk management represents a challenge for organizations, as it includes environmental, social, and governance (ESG) issues that can negatively impact organizations’ investments. This article shows a general approach for prioritizing organizational risks focused on sustainability, which is applied in a particular case. Based on the analysis of global reports such as the “Global Sustainable Development Report”, “Enterprise Risk Management-Integrating with Strategy and Performance”, and the “Global Risk Report”, five typologies of organizational risks with a focus on sustainability (geopolitical, economic, social, technological, and environmental) that support the concern for sustainability in organizations are characterized, taking into account viability and equitability. Additionally, some sub-risks are proposed for each characterized typology of risk. Subsequently, the application of paired surveys assigned to a group of experts formed by executives from the service sector, auditing and consulting firms, the oil and gas sector, the manufacturing sector, and the financial sector is carried out; the responses obtained are consolidated and used in this study as input for the application of DEMATEL and AHP methods to prioritize risks and sub-risks, respectively. The result obtained via the DEMATEL method is the following risk prioritization: (1) economic, (2) geopolitical, (3) social, (4) technological, and (5) environmental. Using the AHP method, the sustainability sub-risks with the highest level of prioritization for each typology of risk are (1) massive data fraud or theft incident (technological risk), (2) deficit in economic growth (economic risk), (3) water depletion (environmental risk), (4) lack of ethics in the conduct of business (geopolitical risk), and (5) chemical safety (social risk). The sensitivity analysis presents positive and negative values, indicating that the positive results do not generate substantial changes between the characterized sub-risks. On the other hand, the negative results indicate a notable decrease in the relative importance of the sub-risks. It is crucial to highlight that the observed variations remain within realistic limits and reflect the uncertainty inherent in decision-making in a dynamic environment.
Computer Vision-Based Real-Time Identification of Vehicle Loads for Structural Health Monitoring of Bridges
Authors: Yang Jiaxin , Bao Yan , Sun Zhe , Meng Xiaolin
Published: January 2024

Coupling effects of various loading conditions can cause deflections, settlements and even failure of in-service bridges. Although it is one of the most critical loads, unfortunately, loading conditions of moving vehicles are difficult to capture in real time by bridge monitoring systems currently in place for sustainable operation. To fully understand the status of a bridge, it is essential to obtain instantaneous vehicle load distributions in a dynamic traffic environment. Although there are some methods that can identify overweight vehicles, the captured vehicle-related information is scattered and incomplete and thus cannot support effective bridge structural health monitoring (BSHM). This study proposes a noncontact, vision-based approach to identification of vehicle loads for real-time monitoring of bridge structural health. The proposed method consists of four major steps: (1) establish a dual-object detection model for vehicles using YOLOv7, (2) develop a hybrid coordinate transformation model on a bridge desk, (3) develop a multiobject tracking model for real-time trajectory monitoring of moving vehicles, and (4) establish a decision-level fusion model for fusing data on vehicle loads and positions. The proposed method effectively visualizes the 3D spatiotemporal vehicular-load distribution with low delay at a speed of over 30FPS. The results show that the hybrid coordinate transformation ensures that the vehicle position error is within 1 m, a 5-fold reduction compared with the traditional method. Wheelbase is calculated through dual-object detection and transformation and is as the primary reference for vehicle position correction. The trajectory and real-time speed of vehicles are preserved, and the smoothed speed error is under 5.7%, compared with the speed measured by sensors. The authors envision that the proposed method could constitute a new approach for conducting real-time SHM of in-service bridges.
Implementability of BIM Technology in Light of Literature Studies and Analyses of the Construction Market
Authors: Szafranko Elżbieta , Jurczak Marlena
Published: January 2024

In 2014, the European Union Directive 2014/24/EU was issued, which has provisions including information about the use of electronic technology for modelling buildings, referred to as BIM. According to various sources in the literature, with the help of BIM technology building designs can be prepared more quickly, while avoiding errors; it is also possible to generate and control any amendments in a building blueprint, promptly estimate costs of the building works planned, and implement a variety of activities connected with the maintenance of a new building once it is erected. In some EU states, initiatives have been launched leading to the introduction of BIM technology in public procurement, and the technology itself is now gaining popularity. However, the adaptation of this new technology is not always met with enthusiasm, and its implementation is hindered by many obstacles. The aim of this article is to conduct a broader analysis of the possibilities of BIM development in the construction industry based on data obtained from companies operating in the construction industry. The research used analysis of source materials, surveys, and targeted interviews. The study has demonstrated several barriers, of which the most important ones proved to be of financial nature, formal and legal considerations, and mentality, namely the unwillingness to learn, adopt novel solutions, and switch to a new way of working. The research was supported by a SWOT analysis.
Developing Climate Change Adaptation Plans for the Health Sector at the Subnational Level
Authors: Bocanegra Rios Evelin Elizabeth , Thomson Craig S.
Published: January 2024

Climate change impacts represent threats to the population worldwide, and the health sector which is responsible for taking care of their life and health will also be impacted. While the consequences of these impacts are imminent, little or nothing has been done by the health sector at national and subnational levels worldwide to prepare to respond to them. Therefore, health adaptation planning in regard to climate change is crucial to building climate-resilient health systems. This research aims to propose an approach containing phases and steps for developing a Health Adaptation Plan at a subnational level. The methodology involved the collection and analysis of several guidelines and tools provided by the WHO and other relevant organisations. This is supplemented by analysis of existing Health Adaptation Plans at national and subnational levels developed in different governmental organisations and systems worldwide since 2003. The findings included four phases and twelve steps for developing Health Adaptation Plans at subnational-level organisations in the health sector. The s establishes guidance for subnational organisations within the health sector that are planning to develop a Health Adaptation Plan.
Linear Relations between Numbers of Terms and First Terms of Sums of Consecutive Squared Integers Equal to Squared Integers
Authors: Pletser Vladimir
Published: January 2024

The classical problem of finding all integers a and M such that the sums of M consecutive squared integers a+i2 equal the squared integer s2, where M is the number of terms in the sum, a2 the first term and a≥1, 0≤i≤M−1, yields remarkable regular linear features when plotting the values of M as a function of a. These linear features correspond to groupings of pairs of a values for successive same values of M found on either side of straight lines of equation μM=2a+c, where c is an integer constant and μ a parameter taking some rational values, called allowed values. We find expressions of a and s as a function of M for the allowed values of μ and M and parametric expressions of a, M, and s. Further, Pell equations deduced from the conditions of M are solved to find the allowed values of μ and to provide all solutions in a and M. These results yield new insights into the overall properties of the classical problem of the sums of consecutive squared integers equal to squared integers and allow us to solve this problem completely by providing all solutions in infinite families.
Assessment of Secondary Sulfate Aqueous-Phase Formation Pathways in the Tropical Island City of Haikou: A Chemical Kinetic Perspective
Authors: Wang Chen , Luo Li , Xu Zifu , Liu Shuhan , Li Yuxiao , Ni Yuanzhe , Kao Shuh-Ji
Published: January 2024

Sulfate (SO42−) is an essential chemical species in atmospheric aerosols and plays an influential role in their physical–chemical characteristics. The mechanisms of secondary SO42− aerosol have been intensively studied in air-polluted cities. However, few studies have focused on cities with good air quality. One-year PM2.5 samples were collected in the tropical island city of Haikou, and water-soluble inorganic ions, as well as water-soluble Fe and Mn, were analyzed. The results showed that non-sea-salt SO42− (nss-SO42−) was the dominant species of water-soluble inorganic ions, accounting for 40–57% of the total water-soluble inorganic ions in PM2.5 in Haikou. The S(IV)+H2O2 pathway was the main formation pathway for secondary SO42− in wintertime in Haikou, contributing to 57% of secondary SO42− formation. By contrast, 54% of secondary SO42− was produced by the S(IV)+Fe×Mn pathway in summer. In spring and autumn, the S(IV)+H2O2, S(IV)+Fe×Mn, and S(IV)+NO2 pathways contributed equally to secondary SO42− formation. The ionic strength was the controlling parameter for the S(IV)+NO2 pathway, while pH was identified as a key factor that mediates the S(IV)+H2O2 and S(IV)+Fe×Mn pathways to produce secondary SO42−. This study contributes to our understanding of secondary SO42− production under low PM2.5 concentrations but high SO42− percentages.
Increased Perfluorooctanesulfonate (PFOS) Toxicity and Accumulation Is Associated with Perturbed Prostaglandin Metabolism and Increased Organic Anion Transport Protein (OATP) Expression
Authors: Williams Lanie , Hamilton Matthew , Edin Matthew , Lih Fred , Eccles-Miller Jazmine , Tharayil Nishanth , Leonard Elizabeth , Baldwin William
Published: January 2024

Perfluorooctanesulfonate (PFOS) is a widespread environmental pollutant with a long half-life and clearly negative outcomes on metabolic diseases such as fatty liver disease and diabetes. Male and female Cyp2b-null and humanized CYP2B6-transgenic (hCYP2B6-Tg) mice were treated with 0, 1, or 10 mg/kg/day PFOS for 21 days, and surprisingly it was found that PFOS was retained at greater concentrations in the serum and liver of hCYP2B6-Tg mice than those of Cyp2b-null mice, with greater differences in the females. Thus, Cyp2b-null and hCYP2B6-Tg mice provide new models for investigating individual mechanisms for PFOS bioaccumulation and toxicity. Overt toxicity was greater in hCYP2B6-Tg mice (especially females) as measured by mortality; however, steatosis occurred more readily in Cyp2b-null mice despite the lower PFOS liver concentrations. Targeted lipidomics and transcriptomics from PFOS-treated Cyp2b-null and hCYP2B6-Tg mouse livers were performed and compared to PFOS retention and serum markers of toxicity using PCA. Several oxylipins, including prostaglandins, thromboxanes, and docosahexaenoic acid metabolites, are associated or inversely associated with PFOS toxicity. Both lipidomics and transcriptomics indicate PFOS toxicity is associated with PPAR activity in all models. GO terms associated with reduced steatosis were sexually dimorphic with lipid metabolism and transport increased in females and circadian rhythm associated genes increased in males. However, we cannot rule out that steatosis was initially protective from PFOS toxicity. Moreover, several transporters are associated with increased retention, probably due to increased uptake. The strongest associations are the organic anion transport proteins (Oatp1a4-6) genes and a long-chain fatty acid transport protein (fatp1), enriched in female hCYP2B6-Tg mice. PFOS uptake was also reduced in cultured murine hepatocytes by OATP inhibitors. The role of OATP1A6 and FATP1 in PFOS transport has not been tested. In summary, Cyp2b-null and hCYP2B6-Tg mice provided unique models for estimating the importance of novel mechanisms in PFOS retention and toxicity.
Bacillus anthracis, “la maladie du charbon”, Toxins, and Institut Pasteur
Authors: Goossens Pierre L.
Published: January 2024

Institut Pasteur and Bacillus anthracis have enjoyed a relationship lasting almost 120 years, starting from its foundation and the pioneering work of Louis Pasteur in the nascent fields of microbiology and vaccination, and blooming after 1986 following the molecular biology/genetic revolution. This contribution will give a historical overview of these two research eras, taking advantage of the archives conserved at Institut Pasteur. The first era mainly focused on the production, characterisation, surveillance and improvement of veterinary anthrax vaccines; the concepts and technologies with which to reach a deep understanding of this research field were not yet available. The second period saw a new era of B. anthracis research at Institut Pasteur, with the anthrax laboratory developing a multi-disciplinary approach, ranging from structural analysis, biochemistry, genetic expression, and regulation to bacterial-host cell interactions, in vivo pathogenicity, and therapy development; this led to the comprehensive unravelling of many facets of this toxi-infection. B. anthracis may exemplify some general points on how science is performed in a given society at a given time and how a scientific research domain evolves. A striking illustration can be seen in the additive layers of regulations that were implemented from the beginning of the 21st century and their impact on B. anthracis research. B. anthracis and anthrax are complex systems that raise many valuable questions regarding basic research. One may hope that B. anthracis research will be re-initiated under favourable circumstances later at Institut Pasteur.
Multiplicity Distributions and Modified Combinants in the Multipomeron Model of pp Interaction at High Energies
Authors: Vechernin Vladimir , Andronov Evgeny , Kovalenko Vladimir , Puchkov Andrei
Published: January 2024

The multiplicity distributions of charged particles and their combinants for pp collisions at LHC energies are studied within the Multipomeron Exchange Model (MEM) that takes into account the phenomenon of string fusion. It is shown that the use of Gaussian-type distributions for multiplicity distributions at a fixed number of pomerons allows, within the MEM framework, the reproduction of the resulting multiplicity distributions and the oscillatory behavior of combinants, found in the ALICE and CMS pp collision data at LHC energies. It is important that in the proposed approach, the parameters of these Gaussian-type distributions are not considered free, but are calculated from the two-particle correlation function of a single string.
The Anti-SARS-CoV-2 IgG1 and IgG3 Antibody Isotypes with Limited Neutralizing Capacity against Omicron Elicited in a Latin Population a Switch toward IgG4 after Multiple Doses with the mRNA Pfizer–BioNTech Vaccine
Authors: Espino Ana M. , Armina-Rodriguez Albersy , Alvarez Laura , Ocasio-Malavé Carlimar , Ramos-Nieves Riseilly , Rodriguez Martinó Esteban I. , López-Marte Paola , Torres Esther A. , Sariol Carlos A.
Published: January 2024

The aim of this study was to analyze the profiles of IgG subclasses in COVID-19 convalescent Puerto Rican subjects and compare these profiles with those of non-infected immunocompetent or immunocompromised subjects that received two or more doses of an mRNA vaccine. The most notable findings from this study are as follows: (1) Convalescent subjects that were not hospitalized developed high and long-lasting antibody responses. (2) Both IgG1 and IgG3 subclasses were more prevalent in the SARS-CoV-2-infected population, whereas IgG1 was more prevalent after vaccination. (3) Individuals that were infected and then later received two doses of an mRNA vaccine exhibited a more robust neutralizing capacity against Omicron than those that were never infected and received two doses of an mRNA vaccine. (4) A class switch toward the “anti-inflammatory” antibody isotype IgG4 was induced a few weeks after the third dose, which peaked abruptly and remained at high levels for a long period. Moreover, the high levels of IgG4 were concurrent with high neutralizing percentages against various VOCs including Omicron. (5) Subjects with IBD also produced IgG4 antibodies after the third dose, although these antibody levels had a limited effect on the neutralizing capacity. Knowing that the mRNA vaccines do not prevent infections, the Omicron subvariants have been shown to be less pathogenic, and IgG4 levels have been associated with immunotolerance and numerous negative effects, the recommendations for the successive administration of booster vaccinations to people should be revised.
Genotype Sequencing and Phylogenetic Analysis Revealed the Origins of Citrus Yellow Vein Clearing Virus California Isolates
Authors: Sun Yong-Duo , Yokomi Raymond
Published: January 2024

The Citrus yellow vein clearing virus (CYVCV) causes a viral disease that has been reported in some citrus-growing regions in countries in Eurasia including Pakistan, India, Türkiye, Iran, China, and South Korea. Recently, CYVCV was detected in a localized urban area in a town in the middle of California’s citrus-growing region and marks the first occurrence of the virus in North America. CYVCV has been reported to be spread by aphid and whitefly vectors and is graft and mechanically transmitted. Hence, it is an invasive pathogen that presents a significant threat to the California citrus industry, especially lemons, which are highly symptomatic to CYVCV. To elucidate the origin of the CYVCV California strain, we used long-read sequencing technology and obtained the complete genomes of three California CYVCV isolates, CA1, CA2, and CA3. The sequences of these isolates exhibited intergenomic similarities ranging from 95.4% to 97.4% to 54 publicly available CYVCV genome sequences, which indicated a relatively low level of heterogeneity. However, CYVCV CA isolates formed a distinct clade from the other isolates when aligned against other CYVCV genomes and coat protein gene sequences as shown by the neighbor network analysis. Based on the rooted Maximum Likelihood phylogenetic trees, CYVCV CA isolates shared the most recent common ancestor with isolates from India/South Asia. Bayesian evolutionary inferences resulted in a spatiotemporal reconstruction, suggesting that the CYVCV CA lineage diverged from the Indian lineage possibly around 1995. This analysis placed the origin of all CYVCV to around 1990, with South Asia and/or Middle East as the most plausible geographic source, which matches to the first discovery of CYVCV in Pakistan in 1988. Moreover, the spatiotemporal phylogenetic analysis indicated an additional virus diffusion pathway: one from South Asia to China and South Korea. Collectively, our phylogenetic inferences offer insights into the probable dynamics of global CYVCV dissemination, emphasizing the need for citrus industries and regulatory agencies to closely monitor citrus commodities crossing state and international borders.
Clinical Significance of Elevated KSHV Viral Load in HIV-Related Kaposi’s Sarcoma Patients in South Africa
Authors: Tibenderana Rebecca Monica , Blumenthal Melissa Jayne , Bukajumbe Emmanuel , Schäfer Georgia , Mohamed Zainab
Published: January 2024

Kaposi’s sarcoma (KS) is an AIDS-defining illness caused by Kaposi’s sarcoma-associated herpesvirus (KSHV) predominantly in the context of HIV-related immune suppression. We aimed to explore the usefulness of KSHV DNA viral load (VL) measurement in predicting the severity, response to treatment and outcome of KS. We retrospectively assessed a cohort of KS patients (n = 94) receiving treatment at Groote Schuur Hospital, Cape Town, South Africa. Demographic and clinical data, KS staging and response to treatment were extracted from patient files, while long-term survival was ascertained from hospital records. KSHV serology and VL and hIL-6 were determined empirically from patients’ blood. All patients were HIV-positive adults, the majority of whom were on HAART at the time of recruitment. KSHV VL was detectable in 65 patients’ blood (median: 280.5/106 cells (IQR: 69.7–1727.3)) and was highest in patients with S1 HIV-related systemic disease (median 1066.9/106 cells, IQR: 70.5–11,269.6). KSHV VL was associated with the S1 stage in a binomial regression controlling for confounders (adjusted odds ratio 5.55, 95% CI: 1.28–24.14, p = 0.022). A subset of six patients identified to have extremely high KSHV VLs was predominantly T1 stage with pulmonary KS, and most had died at follow-up. In our cohort, elevated KSHV VL is associated with systemic HIV-related illness in KS disease. Extremely high KSHV VLs warrant further investigation for patients potentially requiring intensive treatment and investigation for progression or diagnosis of concurrent KSHV lytic syndromes.
Vaccine Hesitancy among European Parents—Psychological and Social Factors Influencing the Decision to Vaccinate against HPV: A Systematic Review and Meta-Analysis
Authors: Achimaș-Cadariu Teodora , Pașca Andrei , Jiboc Nicoleta-Monica , Puia Aida , Dumitrașcu Dan Lucian
Published: January 2024

Background: Due to low adherence to HPV vaccination programs, the European region struggles with vaccination rates lower than 30% among the targeted population. The present report investigated various socio-demographic and psychological factors influencing European parents’ hesitancy towards vaccinating their children. Methods: As of September 2023, four databases were searched. After applying inclusion criteria, all articles comparing psychological and socio-demographic factors in parents who vaccinate or do not vaccinate their children were included. Results: Twenty-five primary publications met the criteria, totaling 385,460 responders, of which 311,803 want to or have already vaccinated their child, and 73,555 do not wish to do so. Immigrant and employment status, religion, age of parents and the child’s gender seemed to influence their decision to vaccinate their child. Previous experience with vaccines, perceived safety and efficacy and the mother’s previous abnormal CCS results also impacted their decision. The caregivers’ education, gender, civil status, number of children, family history of neoplasia or mother’s CCS attendance did not influence their hesitancy to vaccine. Conclusion: Multiple demographic, social, economic and psychological barriers involved in the parents’ hesitancy to vaccinate their children against the HPV virus were highlighted. Specific at-risk categories that need to be targeted with information, education and vaccination campaigns were identified.
Effects of Dietary Supplementation of Stimbiotics to Sows on Lactation Performance, Immune Function, and Anti-Inflammatory and Antioxidant Capacities during Late Gestation and Lactation
Authors: Li Jing , Chen Wen-Ning , Sun Wen-Juan , Cordero Gustavo , Hasan Shah , Bontempo Valentino , Xiao Jun-Feng , Li Yan-Pin , Pi Yu , Li Xi-Long , Jiang Xian-Ren
Published: January 2024

Stimbiotic supplementation may provide an innovative feed additive solution to accelerate the proliferation of beneficial fiber-degrading bacteria in the distal intestine and the utilization of dietary fiber. Optimal utilization of dietary fiber has multiple benefits for gut health and nutrient utilization. This study was conducted to evaluate the late gestation and lactation performance, the plasma, colostrum, and milk immunoglobulin (IgA, IgG, and IgM) concentrations, and the anti-inflammatory and antioxidant biomarkers in plasma of sows fed with or without a stimbiotic during the late gestation and lactation phase. A total of 40 sows were allocated to two treatment groups: control (CT) with no supplementation or 100 mg/kg stimbiotic (VP), with 20 sows per treatment. Sows were fed the treatment diets from d 85 of gestation to d 28 of lactation. In the results, the average daily weight gain of piglets during lactation was greater from sows fed in the VP group compared to that in the CT group (p < 0.05). The plasma concentrations of IgM at farrowing and IgG at weaning of the sows fed the diet with the stimbiotic supplementation were much higher than those in the CT sows (p < 0.05), respectively. In addition, the dietary stimbiotic increased the concentrations of IgM in the colostrum and of IgA and IgM in the milk at d 14 of lactation (p < 0.05). Plasma concentrations of malondialdehyde (MDA) on d 0 and d 28 of lactation tended to be lower in sows fed the VP diets compared with those of the sows fed the CT diets. Thus, our study indicated that stimbiotic supplementation could improve the daily weight gain of piglets and the immune function of sows in lactation.