bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative analysis of albumin quotient and total CSF protein in immune-mediated neuropathies: a multicenter study on diagnostic implications
Authors: Seeliger Tabea , Gingele Stefan , Güzeloglu Yunus Emre , Heitmann Lena , Lüling Benjamin , Kohle Felix , Preßler Hannah , Stascheit Frauke , Motte Jeremias , Fisse Anna Lena , Grüter Thomas , Pitarokoili Kalliopi , Skripuletz Thomas
Published: January 2024

Introduction

Blood-cerebrospinal fluid (CSF) barrier dysfunction is pivotal for diagnosing immune-mediated neuropathies, especially in spinal nerve root inflammation. Typically, either total CSF protein or the CSF to serum albumin ratio (QAlb) is measured. Total CSF protein measurements have limitations, notably its fixed reference value regardless of age, in contrast to the age-dependent reference for QAlb. Our goal was to evaluate both markers in patients with immune-mediated neuropathies.

Methods

In our multicenter research, we collected retrospective CSF data from patients suffering from immune-mediated neuropathies across four German research centers. These parameters were analyzed in relation to their clinical characteristics.

Results

Out of 419 samples, 36 (8.6%) displayed a notable variation between total CSF protein and QAlb values. A detailed analysis revealed that patients displaying elevated QAlb but normal total CSF protein levels were significantly younger at disease onset (p = 0.01), at the time of diagnosis (p = 0.005), and when undergoing lumbar puncture (p = 0.001) compared to patients with elevated CSF protein and normal QAlb levels. These effects were especially evident for the subgroup of samples derived by female patients.

Discussion

Our work confirms the crucial role of QAlb in diagnosing immune-mediated neuropathies and particularly its efficacy as a marker for evaluating the blood-CSF barrier in patients with an earlier disease onset. Considering the significance of the albumin quotient, its assessment is especially advisable in younger patients of female sex to avoid missing a potential barrier dysfunction that might be falsely negative when using total protein.
Sensorimotor-linked reward modulates smooth pursuit eye movements in monkeys
Authors: Hu Yongxiang , Wang Huan , Joshua Mati , Yang Yan
Published: January 2024

Reward is essential for shaping behavior. Using sensory cues to imply forthcoming rewards, previous studies have demonstrated powerful effects of rewards on behavior. Nevertheless, the impact of reward on the sensorimotor transformation, particularly when reward is linked to behavior remains uncertain. In this study, we investigated how reward modulates smooth pursuit eye movements in monkeys. Three distinct associations between reward and eye movements were conducted in independent blocks. Results indicated that reward increased eye velocity during the steady-state pursuit, rather than during the initiation. The influence depended on the particular association between behavior and reward: a faster eye velocity was linked with reward. Neither rewarding slower eye movements nor randomizing rewards had a significant effect on behavior. The findings support the existence of distinct mechanisms involved in the initiation and steady-state phases of pursuit, and contribute to a deeper understanding of how reward interacts with these two periods of pursuit.
Preparation, characterization, and property evaluation of Hericium erinaceus peptide–calcium chelate
Authors: Gu Haofeng , Liang Lei , Kang Yongning , Yu Rongmiao , Wang Jiahao , Fan Dan
Published: January 2024

Recently, owing to the good calcium bioavailability, peptide–calcium chelates made of various foods have been emerging. Hericium erinaceus, an edible fungus, is rich in proteins with a high proportion of calcium-binding amino acids. Thus, mushrooms serve as a good source to prepare peptide–calcium chelates. Herein, the conditions for hydrolyzing Hericium erinaceus peptides (HP) with a good calcium-binding rate (CBR) were investigated, followed by the optimization of HP–calcium chelate (HP-Ca) preparation. Furthermore, the structure of the new chelates was characterized along with the evaluation of gastrointestinal stability and calcium absorption. Papain and a hydrolysis time of 2 h were selected for preparing Hericium erinaceus peptides, and the conditions (pH 8.5, temperature 55°C, time 40 min, and mass ratio of peptide/CaCl2 4:1) were optimal to prepare HP-Ca. Under this condition, the chelates contained 6.79 ± 0.13% of calcium. The morphology and energy disperse spectroscopy (EDS) analysis showed that HP-Ca was loose and porous, with an obvious calcium element signal. The ultraviolet–visible (UV) absorption and Fourier transform infrared spectroscopy (FT-IR) analysis indicated that calcium possibly chelates to HP via interaction with free -COO- from acidic amino acids and C = O from amide. HP-Ca displayed good stability against stimulated gastrointestinal digestion. Moreover, HP-Ca significantly improved the calcium absorption by Caco-2 epithelial cells. Thus, HP-Ca is a promising Ca supplement with high calcium bioavailability.
Primary pulmonary nuclear protein of the testis midline carcinoma: case report and systematic review with pooled analysis
Authors: Zhao Dong , Cao Wei , Zha Shiqian , Wang Yixuan , Pan Zhou , Zhang Jingyi , Hu Ke
Published: January 2024

Nuclear protein of the testis (NUT) midline carcinoma (NMC) is a rare tumor, with particularly low incidence in the lungs, and a correspondingly poor prognosis. To determine the clinicopathological characteristics, outcomes, and prognostic factors of primary pulmonary NMC, a case was reported and a systematic review was performed. Twenty-nine records, including ours, involving 62 cases, were finally included. The median age at diagnosis was 29.5 years. At presentation, the most common symptoms at presentation were cough (47.50%) and chest/back pain (37.50%). In terms of diagnosis, 32.14% of NMC cases were identified through immunohistochemistry (IHC); However, a greater number of cases may be misdiagnosed initially, and ultimately, the diagnosis of NMC was confirmed through a combination of IHC and fluorescence in situ hybridization (FISH). Despite the clinical application of various chemotherapy-based treatments, the actual effectiveness remains unsatisfactory. Furthermore, Cox regression analysis of multiple factors identified male gender and concurrent presence of pleural effusion as indicators of shorter survival time in patients. These results emphasize the importance of increased diagnostic awareness among clinical and pathology practitioners concerning NMC. While there is currently no established standard for treating NMC, a treatment approach combining multiple methods shows promise for future research. Concurrently, clinical and foundational investigations addressing variables such as gender and the presence of pleural effusion may yield valuable insights into the diagnosis and treatment of NMC.
Primary site surgery of de novo stage IV HER2-positive breast cancer in the era of new drug treatments
Authors: Lu Guanyu , Jia Lin , Yang Ruohan , Liu Yutong , Lv Zheng , Cui Jiuwei
Published: January 2024

Objective

The surgical treatment of the primary site has been a subject of controversy in patients with de novo metastatic breast cancer. In recent years, studies using large databases and retrospective analyses have provided evidence of the survival benefits of localized surgery for these patients. However, due to the improved prognosis associated with novel antitumor agents and the widespread use of anti-HER2 therapy, it is important to investigate the role of primary site surgery in the context of new drug treatments for stage IV HER2-positive breast cancer.

Methods

This retrospective analysis included patients with metastatic breast cancer at diagnosis who were consulted at the First Hospital of Jilin University between 2016 and 2022. We compared the patients’ clinical and pathological characteristics, treatment regimens, and prognosis between the surgery and non-surgery groups.

Results

A total of 96 patients with stage IV HER2-positive breast cancer were included in the study, with 24 patients (25%) undergoing surgery for the primary lesion. Patients with lower Eastern Cooperative Oncology Group (ECOG) scores, earlier T-stage, metastases confined to one organ/site, and fewer metastases were more likely to undergo surgery. Patients in the surgical group had longer progression-free survival (median 25.7 vs. 15.9 months, p=0.073) and overall survival (median 79.1 vs. 48 months, p=0.073) compared to patients in the non-surgical group, however, there was no statistical difference. Univariate and multivariate Cox regression analysis suggested that the choice of first-line targeted therapy regimens rather than surgical treatment influenced the patients’ prognoses. In the subgroup of patients receiving first-line targeted therapy with trastuzumab plus pertuzumab, the decision to undergo surgery on the primary site did not have a statistically significant effect on prognosis.

Conclusion

Primary site surgery does not improve the prognosis of de novo stage IV HER2-positive breast cancer. In the era of anti-HER2 therapy, primary surgery is not recommended, except in exceptional circumstances.
Epidemiology of thymomas and thymic carcinomas in the United States and Germany, 1999-2019
Authors: Gerber Tiemo Sven , Strobl Stephanie , Marx Alexander , Roth Wilfried , Porubsky Stefan
Published: January 2024

Introduction

Mediastinal tumors, particularly non-neuroendocrine thymic epithelial tumors (TET) are relatively uncommon, posing challenges for extensive epidemiological studies. This study presents a comprehensive analysis of these tumors in the United States (US) and Germany (GER) from 1999 to 2019.

Methods

Patients aged 0-19 (n=478) and ≥20 years (n=17,459) diagnosed with malignant tumors of the anterior mediastinum were identified from the Surveillance, Epidemiology, and End Results registry (SEER) and the Zentrum für Krebsregisterdaten (ZfKD) databases.

Results

Among patients aged ≥20 years, TETs accounted for the most prevalent anterior mediastinal tumors (US/GER: 63%/64%), followed by lymphomas (14%/8%). For patients <20 years, predominant tumors included germ cell tumors (42%/14%), lymphomas (38%/53%), and TETs (10%/27%). The overall annual incidence of thymoma was 2.2/2.64 (US/GER) per million inhabitants and for thymic carcinomas 0.48/0.42. The male-to-female ratio was 1:1.09/1.03, and the mean age 59.48 ± 14.89/61.33 ± 13.94. Individuals with thymomas, but not thymic carcinomas, exhibited a 21%/29% significantly heightened risk of developing secondary malignancies compared to controls with non-thymic primary tumors.

Discussion

This study provides a comparative analysis of anterior mediastinal tumors, particularly TETs, in the US and GER over the past two decades. Furthermore, it highlights a significantly elevated incidence of secondary malignancies in thymoma patients.
Evaluating the predictive significance of systemic immune-inflammatory index and tumor markers in lung cancer patients with bone metastases
Authors: He Jinxian , Liang Gaofeng , Yu Hongyan , Lin Chengbin , Shen Weiyu
Published: January 2024

Objective

This study aims to develop a predictive model for identifying lung cancer patients at elevated risk for bone metastases, utilizing the Unified Immunoinflammatory Index and various tumor markers. This model is expected to facilitate timely and effective therapeutic interventions, especially in the context of the growing significance of immunotherapy for lung cancer treatment.

Methods

A retrospective analysis was conducted on 324 lung cancer patients treated between January 2019 and January 2021. After meeting the inclusion criteria, 241 patients were selected, with 56 exhibiting bone metastases. The cohort was divided into a training group (169 patients) and a validation group (72 patients) at a 7:3 ratio. Lasso regression was employed to identify critical variables, followed by logistic regression to construct a Nomogram model for predicting bone metastases. The model’s validity was ascertained through internal and external evaluations using the Concordance Index (C-index) and Receiver Operating Characteristic (ROC) curve.

Results

The study identified several factors influencing bone metastasis in lung cancer, such as the Systemic Immune-Inflammatory Index (SII), Carcinoembryonic Antigen (CEA), Neuron Specific Enolase (NSE), Cyfra21-1, and Neutrophil-to-Lymphocyte Ratio (NLR). These factors were incorporated into the Nomogram model, demonstrating high validation accuracy with C-index scores of 0.936 for internal and 0.924 for external validation.

Conclusion

The research successfully developed an intuitive and accurate Nomogram prediction model utilizing clinical indicators to predict the risk of bone metastases in lung cancer patients. This tool can be instrumental in aiding clinicians in developing personalized treatment plans, thereby optimizing patient outcomes in lung cancer care.
Clinical aspects of mastication myalgia—an overview
Authors: Barjandi Golnaz , Svedenlöf Johanna , Jasim Hajer , Collin Malin , Hedenberg-Magnusson Britt , Christidis Nikolaos , Ernberg Malin
Published: January 2024

Mastication myalgia is the most common cause of non-odontogenic pain in the orofacial region and is often associated with a reduced quality of life. The purpose of this review is to provide an overview of the clinical aspects of myalgia based on available research. The review includes epidemiological, diagnostic, and etiological aspects. In addition, the potential risk factors related to the transition from acute to chronic myalgia are explored and treatment strategies are presented for its management. As a result, this review may increase clinical knowledge about mastication myalgia and clarify strategies regarding prevention, diagnostics, and management to improve prognosis and reduce patient suffering.
Adverse event reporting of four anti-Calcitonin gene-related peptide monoclonal antibodies for migraine prevention: a real-world study based on the FDA adverse event reporting system
Authors: Sun Wenfang , Li Yali , Xia Binbin , Chen Jing , Liu Yang , Pang Jingyao , Liu Fang , Cheng Hua
Published: January 2024

Background: Anti-Calcitonin gene-related peptide monoclonal antibodies (anti-CGRP mAbs) have shown significant efficacy in preventing migraine. However, there have been limited reports of adverse events (AEs) after marketing, particularly for eptinezumab launched in 2020. The study aimed to mine and analyze the AE signals with four anti-CGRP mAbs from the United States Food and Drug Administration (FDA) Adverse Event Reporting System (FAERS) database to gain insights into the safety profile of these medications post-marketing.

Methods: All AE reports on the four anti-CGRP mAbs (erenumab, galcanezumab, fremanezumab, and eptinezumab) were retrieved from the FAERS database from the first quarter (Q1) of 2018 to Q1 of 2023. Disproportionality analysis was measured by reporting odd ratio (ROR) and Bayesian confidence propagation neural network (BCPNN) to identify potential AE signals. Comparisons were made between the four drugs in terms of AEs.

Results: A total of 38,515 reports of erenumab, 19,485 reports of galcanezumab, 5,332 reports of fremanezumab, and 2,460 reports of eptinezumab were obtained, mostly reported in the second to third year after launch in the market. The common AEs to erenumab included constipation (17.93%), injection site pain (14.08%), and alopecia (7.23%). The AEs that occurred more frequently with galcanezumab included injection site pain (24.37%), injection site erythema (5.35%), and injection site haemorrhage (4.97%). Common AEs related to fremanezumab were injection site pain (13.10%), injection site erythema (7.02%), and injection site pruritus (5.47%). Fatigue (13.54%), throat irritation (9.02%), and pruritus (8.20%) were the most common AEs with eptinezumab. In addition, there are new AEs that were not listed in the drug instructions but occurred concurrently with multiple drugs, such as Raynaud’s phenomenon, weight increase, menstrual disorders, throat tightness, and paraesthesia oral.

Conclusion: Common AE signals of the four anti-CGRP mAbs and new AE signals were found to provide a reference for clinical drug selection in clinical practice.
Using real-world data to inform dosing strategies of rituximab for pediatric patients with frequent-relapsing or steroid-dependent nephrotic syndrome: a prospective pharmacokinetic-pharmacodynamic study
Authors: Chen Yewei , Shen Qian , Xiong Ye , Dong Min , Xu Hong , Li Zhiping
Published: January 2024

Objectives: Rituximab is frequently used off-label for the treatment of frequent-relapsing/steroid-dependent nephrotic syndrome (FRNS/SDNS). However, the optimal dosing schedules remain undetermined. The objective of this study was to establish a population pharmacokinetic-pharmacodynamic (PK-PD) model in pediatric patients with FRNS/SDNS, and to investigate dosing regimens that provide adequate suppression of B lymphocytes.

Methods: A prospective, open-label, single-center study was conducted in Nephrology Department at Children’s Hospital of Fudan University, and a two-compartment PK model of rituximab in pediatric FRNS/SDNS has been developed previously by our group. CD19+ lymphocyte count profiles were obtained from these patients. The presence of anti-rituximab antibodies was assessed prior to medication in children who had previously received rituximab or during follow-up at the last sampling point for PK analysis. PK-PD analyses were performed to describe the changes of CD19+ lymphocytes, with rituximab assumed to increase their death rate. Monte Carlo simulation was conducted to evaluate different dosing regimens.

Results: In total, 102 measurements of CD19+ lymphocyte counts were available for PK-PD analysis. No detectable levels of anti-rituximab antibodies were observed during the PK follow-up period. A turnover model with saturable stimulatory action of rituximab on the removal of lymphocytes best characterized the relationship between rituximab concentration and CD19+ lymphocyte counts, where the Emax and EC50 were estimated to be 99.6*106/L and 5.87 μg/mL, respectively. Simulations indicated that a single infusion of 750 mg/m2 and 2 infusions of 375 mg/m2 both yielded a 10-week suppression of CD19+ lymphocytes.

Conclusion: This study represents a first attempt to quantitatively describe the PK-PD relationship of rituximab in pediatric patients with FRNS/SDNS, and provide a potential pathway for future precision dosing strategy for rituximab therapy. Further clinical studies are warranted to evaluate the efficacy and safety of different dosing schemes.
Transcriptome analysis and immune gene expression of channel catfish (Ictalurus punctatus) fed diets with inclusion of frass from black soldier fly larvae
Authors: Sankappa Nithin Muliya , Lange Miles D. , Yildirim-Aksoy Mediha , Eljack Rashida , Kucuktas Huseyin , Beck Benjamin H. , Abernathy Jason W.
Published: January 2024

The larval waste, exoskeleton shedding, and leftover feed components of the black soldier fly and its larvae make up the by-product known as frass. In this study, we subjected channel catfish (Ictalurus punctatus) to a 10-week feeding trial to assess how different dietary amounts of frass inclusion would affect both systemic and mucosal tissue gene expression, especially in regard to growth and immune-related genes. Fish were divided in quadruplicate aquaria, and five experimental diets comprising 0, 50, 100, 200, and 300 g of frass per kilogram of feed were fed twice daily. At the end of the trial, liver, head kidney, gill, and intestine samples were collected for gene expression analyses. First, liver and intestine samples from fish fed with a no frass inclusion diet (control), low-frass (50 g/kg) inclusion diet, or a high-frass (300 g/kg) inclusion diet were subjected to Illumina RNA sequencing to determine global differential gene expression among diet groups. Differentially expressed genes (DEGs) included the upregulation of growth-related genes such as glucose-6-phosphatase and myostatin, as well as innate immune receptors and effector molecules such as toll-like receptor 5, apolipoprotein A1, C-type lectin, and lysozyme. Based on the initial screenings of low/high frass using RNA sequencing, a more thorough evaluation of immune gene expression of all tissues sampled, and all levels of frass inclusion, was further conducted. Using targeted quantitative PCR panels for both innate and adaptive immune genes from channel catfish, differential expression of genes was identified, which included innate receptors (TLR1, TLR5, TLR9, and TLR20A), proinflammatory cytokines (IL-1β type a, IL-1β type b, IL-17, IFN-γ, and TNFα), chemokines (CFC3 and CFD), and hepcidin in both systemic (liver and head kidney) and mucosal (gill and intestine) tissues. Overall, frass from black soldier fly larvae inclusion in formulated diets was found to alter global gene expression and activate innate and adaptive immunity in channel catfish, which has the potential to support disease resistance in this species in addition to demonstrated growth benefits.
Stem cell therapy for heart failure in the clinics: new perspectives in the era of precision medicine and artificial intelligence
Authors: Chowdhury Mohammed A. , Zhang Jing J. , Rizk Rodrigue , Chen William C. W.
Published: January 2024

Stem/progenitor cells have been widely evaluated as a promising therapeutic option for heart failure (HF). Numerous clinical trials with stem/progenitor cell-based therapy (SCT) for HF have demonstrated encouraging results, but not without limitations or discrepancies. Recent technological advancements in multiomics, bioinformatics, precision medicine, artificial intelligence (AI), and machine learning (ML) provide new approaches and insights for stem cell research and therapeutic development. Integration of these new technologies into stem/progenitor cell therapy for HF may help address: 1) the technical challenges to obtain reliable and high-quality therapeutic precursor cells, 2) the discrepancies between preclinical and clinical studies, and 3) the personalized selection of optimal therapeutic cell types/populations for individual patients in the context of precision medicine. This review summarizes the current status of SCT for HF in clinics and provides new perspectives on the development of computation-aided SCT in the era of precision medicine and AI/ML.
DPXception: a lightweight CNN for image-based date palm species classification
Authors: Safran Mejdl , Alrajhi Waleed , Alfarhood Sultan
Published: January 2024

Introduction

Date palm species classification is important for various agricultural and economic purposes, but it is challenging to perform based on images of date palms alone. Existing methods rely on fruit characteristics, which may not be always visible or present. In this study, we introduce a new dataset and a new model for image-based date palm species classification.

Methods

Our dataset consists of 2358 images of four common and valuable date palm species (Barhi, Sukkari, Ikhlas, and Saqi), which we collected ourselves. We also applied data augmentation techniques to increase the size and diversity of our dataset. Our model, called DPXception (Date Palm Xception), is a lightweight and efficient CNN architecture that we trained and fine-tuned on our dataset. Unlike the original Xception model, our DPXception model utilizes only the first 100 layers of the Xception model for feature extraction (Adapted Xception), making it more lightweight and efficient. We also applied normalization prior to adapted Xception and reduced the model dimensionality by adding an extra global average pooling layer after feature extraction by adapted Xception.

Results and discussion

We compared the performance of our model with seven well-known models: Xception, ResNet50, ResNet50V2, InceptionV3, DenseNet201, EfficientNetB4, and EfficientNetV2-S. Our model achieved the highest accuracy (92.9%) and F1-score (93%) among the models, as well as the lowest inference time (0.0513 seconds). We also developed an Android smartphone application that uses our model to classify date palm species from images captured by the smartphone’s camera in real time. To the best of our knowledge, this is the first work to provide a public dataset of date palm images and to demonstrate a robust and practical image-based date palm species classification method. This work will open new research directions for more advanced date palm analysis tasks such as gender classification and age estimation.
Workplace bullying and sexual harassment at work among hotel housekeepers in the Balearic Islands (Spain)
Authors: Chela-Alvarez Xenia , Bulilete Oana , García-Buades M. Esther , Ferrer-Perez Victoria A. , Llobera Joan
Published: January 2024

Introduction

Hotel housekeepers are close to being a 100% feminized occupational group in Spain. This fact, coupled with some features of the job, places them at high risk of sexual harassment at work and bullying in the workplace. This study aims to explore experiences of sexual harassment at work and workplace bullying among hotel housekeepers in the Balearic Islands. Second, it aims to describe and estimate the prevalence of both phenomena.

Methods

This is a mixed-methods study. Ten semi-structured interviews were conducted with key informants, and six focus groups were held with hotel housekeepers. Additionally, a quantitative cross-sectional study (n = 1,043) was undertaken.

Results

Most participants in focus groups had been sexually harassed at work. However, they had normalized this kind of situations, not labeling themselves as victims of sexual harassment; and harassment events were seen as unimportant, normal, and unquestioned, as well as being part of their daily work. Hotel housekeepers who were sexually harassed indicated high levels of stress at work and low social support. The prevalence of different workplace bullying behaviors was quite high among hotel housekeepers working in the Balearic Islands. Some were associated with poorer self-rated health, less satisfaction with the job and the salary, lower social support, and higher levels of stress. Despite this, qualitative methods informed us that less severe behaviors were normalized and perceived by hotel housekeepers as intrinsic to their job.

Discussion

The results show the high tolerance to less severe expressions of sexual harassment at work and workplace bullying, as well as difficulties in or reluctance to labeling this kind of experiences as such.
Role-theoretic discourse analysis of German security policy: a case of German parliamentary debate on the mission in Afghanistan
Authors: Ni Xiaoshan
Published: January 2024

This article combines linguistic discourse analysis with role theory to create a role-theoretic discourse analysis framework for German security policy. To illustrate this, we employ topos analysis on 30 plenary minutes of parliamentary debates regarding the International Security Assistance Force mission in Afghanistan, conducted by German Members of Parliament between 2001 and 2014. We interpret their perception of Germany’s roles in light of key behavioral norms related to German security policy. The parliamentary discourse, shaped by topoi, sets the stage for decisions on German military operations abroad. The use of topoi is influenced by dominant thought patterns, particularly the perspective on Germany’s role in security policy held by the political elite. Political decisions, in turn, reflect behavioral preferences guided by these viewpoints. Our research reveals how changes in German security policy are mirrored in the discourse. This discourse is structured around five categories of topoi for legitimizing or delegitimizing military operations abroad: necessity, obligation, self-interest, capability and preparedness, and solution. An evaluation of the use of topoi through the lens of role theory indicates that perceptions of Germany’s role have evolved over time, encompassing roles such as a “civilian power,” a “normal state,” and an “agenda-setting role” in sync with its foreign military engagements.
A protocol for a critical realist synthesis of school mindfulness interventions designed to promote pupils’ mental wellbeing
Authors: Abbott Pamela , Nixon Graeme , Stanley Isabel , D’Ambruoso Lucia
Published: January 2024

Introduction

The review described in this protocol will be the first critical realist review of the literature reporting on the impact of school-based mindfulness interventions on the mental wellbeing of pupils. Mindfulness interventions are increasingly being introduced into schools to promote children’s (and teachers’) wellbeing. Findings from impact evaluations, including systematic reviews and metanalysis, suggest that school-based mindfulness interventions promote pupils’ wellbeing. However, there is a need for further evidence on the underlying causal mechanisms and contexts that explain program outcomes, to provide insight into how mindfulness programs can be successfully implemented in other contexts.

Methods and analysis

A critical realist review methodology will be used to provide a causal interdisciplinary understanding of how school-based mindfulness interventions promote the mental wellbeing of pupils. This will be done through a systematic literature review and extrapolating context, agency, intervention, mechanisms, and outcome configurations. This will enable an understanding of how, in certain contexts, pupils can use the resources offered by a mindfulness intervention knowingly or unknowingly to trigger mechanisms that promote their mental wellbeing and what mechanisms in the context support, restrict or prevent change. We will then use retrodiction and retroduction to develop the most plausible interdisciplinary middle-range theory to explain the findings.

Discussion

The review findings will inform a critical realist evaluation of a mindfulness intervention in schools. The findings from the review will also enable us to inform policymakers and other stakeholders about what conditions need to be in place for mindfulness interventions to promote pupils’ mental wellbeing. We will publish the findings from the review in academic and professional publications, policy briefs, workshops, conferences, and social media.

PROSPERO registration number: CRD42023410484.
Two centuries of vaccination: historical and conceptual approach and future perspectives
Authors: Montero David A. , Vidal Roberto M. , Velasco Juliana , Carreño Leandro J. , Torres Juan P. , Benachi O. Manuel A. , Tovar-Rosero Yenifer-Yadira , Oñate Angel A. , O'Ryan Miguel
Published: January 2024

Over the past two centuries, vaccines have been critical for the prevention of infectious diseases and are considered milestones in the medical and public health history. The World Health Organization estimates that vaccination currently prevents approximately 3.5–5 million deaths annually, attributed to diseases such as diphtheria, tetanus, pertussis, influenza, and measles. Vaccination has been instrumental in eradicating important pathogens, including the smallpox virus and wild poliovirus types 2 and 3. This narrative review offers a detailed journey through the history and advancements in vaccinology, tailored for healthcare workers. It traces pivotal milestones, beginning with the variolation practices in the early 17th century, the development of the first smallpox vaccine, and the continuous evolution and innovation in vaccine development up to the present day. We also briefly review immunological principles underlying vaccination, as well as the main vaccine types, with a special mention of the recently introduced mRNA vaccine technology. Additionally, we discuss the broad benefits of vaccines, including their role in reducing morbidity and mortality, and in fostering socioeconomic development in communities. Finally, we address the issue of vaccine hesitancy and discuss effective strategies to promote vaccine acceptance. Research, collaboration, and the widespread acceptance and use of vaccines are imperative for the continued success of vaccination programs in controlling and ultimately eradicating infectious diseases.
The effect of unpredictability on the perception of breathlessness: a narrative review
Authors: Pavy Fabien , Torta Diana M. , von Leupoldt Andreas
Published: January 2024

Breathlessness is an aversive bodily sensation impacting millions of people worldwide. It is often highly detrimental for patients and can lead to profound distress and suffering. Notably, unpredictable breathlessness episodes are often reported as being more severe and unpleasant than predictable episodes, but the underlying reasons have not yet been firmly established in experimental studies. This review aimed to summarize the available empirical evidence about the perception of unpredictable breathlessness in the adult population. Specifically, we examined: (1) effects of unpredictable relative to predictable episodes of breathlessness on their perceived intensity and unpleasantness, (2) potentially associated neural and psychophysiological correlates, (3) potentially related factors such as state and trait negative affectivity. Nine studies were identified and integrated in this review, all of them conducted in healthy adult participants. The main finding across studies suggested that unpredictable compared to predictable, breathlessness elicits more frequently states of high fear and distress, which may contribute to amplify the perception of unpredictable breathlessness, especially its unpleasantness. Trait negative affectivity did not seem to directly affect the perception of unpredictable breathlessness. However, it seemed to reinforce state fear and anxiety, hence possible indirect modulatory pathways through these affective states. Studies investigating neural correlates of breathlessness perception and psychophysiological measures did not show clear associations with unpredictability. We discuss the implication of these results for future research and clinical applications, which necessitate further investigations, especially in clinical samples suffering from breathlessness.
Comparison of strength profile representations using musculoskeletal models and their applications in robotics
Authors: Sutjipto Sheila , Carmichael Marc G. , Paul Gavin
Published: January 2024

Musculoskeletal models provide an approach towards simulating the ability of the human body in a variety of human-robot applications. A promising use for musculoskeletal models is to model the physical capabilities of the human body, for example, estimating the strength at the hand. Several methods of modelling and representing human strength with musculoskeletal models have been used in ergonomic analysis, human-robot interaction and robotic assistance. However, it is currently unclear which methods best suit modelling and representing limb strength. This paper compares existing methods for calculating and representing the strength of the upper limb using musculoskeletal models. It then details the differences and relative advantages of the existing methods, enabling the discussion on the appropriateness of each method for particular applications.
Innovations in monoclonal antibody-based multipurpose prevention technology (MPT) for the prevention of sexually transmitted infections and unintended pregnancy
Authors: Dohadwala Sarah , Geib Matthew T. , Politch Joseph A. , Anderson Deborah J.
Published: January 2024

Monoclonal antibodies (mAbs) are currently being produced for a number of clinical applications including contraception and the prevention of sexually transmitted infections (STIs). Combinations of contraceptive and anti-STI mAbs, including antibodies against HIV-1 and HSV-2, provide a powerful and flexible approach for highly potent and specific multipurpose prevention technology (MPT) products with desirable efficacy, safety and pharmacokinetic profiles. MAbs can be administered systemically by injection, or mucosally via topical products (e.g., films, gels, rings) which can be tailored for vaginal, penile or rectal administration to address the needs of different populations. The MPT field has faced challenges with safety, efficacy, production and cost. Here, we review the state-of-the-art of mAb MPTs that tackle these challenges with innovative strategies in mAb engineering, manufacturing, and delivery that could usher in a new generation of safe, efficacious, cost-effective, and scalable mAb MPTs.
Future Food—Can Cultivated Meat Save Our Planet?
Authors: Meyer Paula , Szczepanski Lena , Büscher Milan , Schächtele Julia , Fiebelkorn Florian
Published: January 2024

Think about what humans will eat in the future. Could you imagine eating meat produced without the extensive farming of animals? This scenario is not as unlikely as you might think. To protect our planet, we must reduce the irreversible damage we are doing to the environment. Eating less meat is a major part of this, because the way we produce most meat today has a tremendous impact on the environment. Plant-based meat alternatives are already available in stores, but it is hard to imitate the taste and texture of meat if no animals are involved. Therefore, scientists have been working on an alternative, called cultivated meat, that is made from real animal tissue but does not require the death of the animal. Cultivated meat shows great promise to become an environmentally and animal-friendly alternative to conventional meat. However, there are still limitations to cultivated meat products that prevent them from being sold in stores.
Exploring Effective Strategies for ToBRFV Management in Tomato Production: Insights into Seed Transmission Dynamics and Innovative Control Approaches
Authors: Fidan Hakan , Ulusoy Damla , Albezirgan Hande Nur
Published: January 2024

The tomato brown rugose fruit virus (ToBRFV), a formidable tobamovirus, poses a significant threat to tomato production globally. This comprehensive study is dedicated to establishing an integrated control strategy for ToBRFV, encompassing the entire tomato cultivation process from seed to harvest. Initial investigations revealed a 0.8% seed transmission rate of ToBRFV, with viral presence detected in endosperms but not in seed embryos. Employing a multifaceted approach, infected seeds underwent a meticulous disinfection process. Gradual heating in a rotating thermal machine from 20 °C to 72 °C for 3 days resulted in a remarkable 0.3% contamination rate, rendering the virus non-infectious. Furthermore, ToBRFV-infected seeds underwent UV light treatment at 254 nm for 30 min, resulting in a 50% reduction in contamination rates. Chemical disinfectants, including 1% Tsunami 100 (comprising 30–60% acetic acid, 15.2% peroxyacetic acid, and 11.2% hydrogen peroxide) and 1% Biocon A (a combination of potassium peroxymonosulfate with buffer and organic acid), exhibited notable success. This study not only unravels the intricate dynamics of ToBRFV transmission and inactivation but also underscores the efficacy of integrated control measures. The findings provide valuable insights for the sustainable management of ToBRFV, contributing to the resilience of global tomato cultivation against this viral menace.
Unmanned-Aerial-Vehicle Data as an Effective Tool for the Evaluation of Ancient Khorasan and Modern Kabot Spring Wheat Varieties under Different Tillage Systems
Authors: Balážová Kristýna , Kumhálová Jitka , Chyba Jan
Published: January 2024

With the changing climate, there is an increasing emphasis on drought-resistant varieties, including the ability to maintain quality production. As there is also interest in ancient wheat varieties, the aim of this study was to evaluate the growth parameters of the ancient Khorasan (Kamut®) and modern Kabot spring wheat varieties using remote sensing data. Images from unmanned aerial vehicles during four growing seasons were processed. Based on vegetation indices, the growth of these varieties and their response to meteorological conditions were evaluated, as well as the ability to resist drought and higher temperatures with respect to specific soil conditions under conventional (CT), minimum (MTC), and minimization (MTD) tillage systems. It was found that Khorasan had the lowest values of the vegetation indices on the CT variant in the dry years 2022 and 2023. On the contrary, in the previous wet years, 2020 and 2021, both varieties showed similar results. Regarding water stress, the CT variant was also the least suitable for ancient Khorasan (average Crop Water Stress Index = CWSI = 0.38). On the contrary, this variant seems to be suitable for the modern Kabot variety (CWSI = 0.29), while no significant difference between tillage variants was found for this variety. In general, water stress was easily detectable from the observed parameters in the growth phase of stem elongation (R2 up to 0.88). Regarding the individual methods of tillage and water stress, the ancient variety Khorasan performed the worst with the CT variant. MTD appeared to be the best tillage method for Khorasan cultivation in terms of water management.
Cross-Sectional Survey of Public Perception of Commercial Greyhound Racing in New Zealand
Authors: Cameron Kristie E. , Vaughan Alison , McAninch Marie J. , Briden Kayla , Dale Arnja
Published: January 2024

The commercial greyhound racing industry in New Zealand is struggling with an eroding social license and ‘on-notice’ status. Multiple independent reviews of the industry have identified ongoing issues of animal welfare during and between races, euthanasia decisions, poor data tracking, a lack of transparency and problems with rehoming dogs, resulting in New Zealand animal advocacy agencies and the general public questioning the continuation of greyhound racing. The current paper assessed the New Zealand public’s awareness and familiarity with commercial greyhound racing, identified current levels of public support or opposition for racing, and provided context in terms of engagement with greyhound racing using a comprehensive survey of a robust sample of New Zealanders. The results confirm that the social license of the greyhound industry is under challenge with most respondents expressing disagreement with or lack of knowledge of current industry practices and indicating they would vote in support of a ban. There is scope for increasing public acceptability by addressing welfare issues, increasing awareness of positive industry practices, and encouraging transparency of the greyhound racing agency. However, as greyhound racing is on the decline worldwide, calls are likely to continue for a phase-out of commercial greyhound racing.
An Investigation of Meat Eating in Samples from Australia and Germany: The Role of Justifications, Perceptions, and Empathy
Authors: Northrope Katherine , Howell Tiffani , Kashima Emiko S. , Buttlar Benjamin , Sproesser Gudrun , Ruby Matthew B.
Published: January 2024

Despite concerns about animal welfare, and health and environmental issues associated with eating meat, meat consumption has continued to increase worldwide, including in Australia. One exception to this is Germany, with 2021 meat consumption levels being the lowest in the last 30 years. This pre-registered study investigated socio-cultural variables associated with meat consumption in Germany (n = 399) and Australia (n = 399) in a cross-sectional online survey. Participants reported levels of current and intended meat consumption, and they completed measures of speciesism, motivations to eat meat, empathy, animal farming perceptions, perceived behavioural control (PBC) over meat eating, and avoidance and dissociation regarding the animal origins of meat. In both Australia and Germany, enjoying the taste of meat positively predicted consumption and empathy towards farmed animals negatively predicted consumption. PBC was a strong positive predictor of intentions to reduce meat consumption in both countries. Empathy and liking the taste of meat were among the best predictors of red meat and poultry consumption, suggesting that interventions to reduce meat consumption may work best by targeting these factors while also increasing people’s sense of control over their food choices.
Automated Image Analysis for Detection of Coccidia in Poultry
Authors: Kellogg Isaac , Roberts David L. , Crespo Rocio
Published: January 2024

Coccidiosis, caused by the protozoan Eimeria sp., is one of the most common and costly diseases impacting the poultry industry. To establish effective control measures, it is essential to identify these protozoa. Typical methods for identifying and determining the severity of the protozoal infection include intestinal lesion scoring or enumeration of the protozoal oocysts in fecal samples. Standard analysis methods require highly skilled technicians or veterinarians to manually identify and manually enumerate these protozoal parasites. This process is labor intensive, time-consuming, and susceptible to human error. None of the current methods available, including molecular flow cytometry or even digital image analysis, can determine if an oocyst is sporulated or not. Oocysts are not infectious until they sporulate. The goal of this study was to design an automated model using Artificial Intelligence (AI) to expedite the process of enumeration, improve the efficiency and accuracy of the species identification, and determine the ability of the oocysts to infect. To this end, we trained and evaluated computer vision models based on the Mask RCNN neural network architecture. A model was trained to detect and differentiate three species and to determine sporulation for each (totaling six detection groups). This model achieved a mean relative percentage difference (RPD) of 5.64%, representing a slight overcount compared to manual counts, averaging across all groups. The mean RPD for each group individually fell within a range from −33.37% to 52.72%. These results demonstrate that these models were speedy and had high agreement with manual counts, with minimal processing of field-quality samples. These models also could differentiate the sporulation status of the oocysts, providing critical diagnostic information for potential field applications.
Dynamic Expression of Follicle-Stimulating Hormone and Estrogen mRNA Receptors Associated with microRNAs 34a and -let-7c in Canine Follicles during the Estrous Cycle
Authors: De los Reyes Monica , Dettleff Phillip , Palomino Jaime , Peralta Oscar A. , Vergara Ana
Published: January 2024

The genes encoding for estrogen receptor (ESR2) and follicle-stimulating hormone receptor (FSHR) play crucial roles in ovarian follicular development. This study aimed to determine the expression levels of miRNAs predicted against FSHR and ESR2 mRNAs in follicular cells related to their target genes during the estrous cycle in canines. Antral follicles were dissected from 72 ovaries following ovariohysterectomies. MiRNAs regulating FSHR and ESR2 genes were selected from miRNA databases, and mature miRNA and mRNA expression profiling was performed using real-time polymerase chain reaction (PCR). The best miRNA for each target gene was selected considering the quantitative PCR (qPCR) performance and target prediction probability, selecting only miRNAs with a binding p-value of 1.0, and choosing cfa-miR-34a and cfa-let-7c for FSHR and ESR2, respectively. The expression levels comparing the different phases of the estrous cycle were evaluated using ANOVA. Pearson correlations between the expression pattern of each miRNA and their target genes were performed. Each miRNA and its target genes were expressed in the granulosa cells in all estrous phases. FSHR remained low in anestrus and proestrus, increased (p < 0.05) to the highest level in estrus, and decreased (p < 0.05) in diestrus. ESR2 showed the same trend as FSHR, with the highest (p < 0.05) expression in estrus and the lowest (p < 0.05) in anestrus and proestrus. A tendency for an inverse relationship was observed between the expression of miR-34a and FSHR only in the anestrus phase, while an inverse correlation (r = −0.8) was found between miRNA-7c and ESR2 (p < 0.01). The expression profile of miR-34a and miR-let-7c and their predicted target genes of dog ovarian follicles throughout the estrous cycle observed in this study suggest a role in the transcriptional regulation of FSHR and ESR2, which is the first evidence of the involvement of these miRNAs in the canine follicular function.