bluuebunny/crossref_metadata_2025 huggingface dataset:
In Vivo Plant Bio-Electrochemical Sensor Using Redox Cycling
Authors: Dotan Tali , Jog Aakash , Kadan-Jamal Kian , Avni Adi , Shacham-Diamand Yosi
Published: February 2023

This work presents an in vivo stem-mounted sensor for Nicotiana tabacum plants and an in situ cell suspension sensor for Solanum lycopersicum cells. Stem-mounted sensors are mechanically stable and less sensitive to plant and air movements than the previously demonstrated leaf-mounted sensors. Interdigitated-electrode-arrays with a dual working electrode configuration were used with an auxiliary electrode and an Ag/AgCl quasi-reference electrode. Signal amplification by redox cycling is demonstrated for a plant-based sensor responding to enzyme expression induced by different cues in the plants. Functional biosensing is demonstrated, first for constitutive enzyme expression and later, for heat-shock-induced enzyme expression in plants. In the cell suspension with redox cycling, positive detection of the enzyme β-glucuronidase (GUS) was observed within a few minutes after applying the substrate (pNPG, 4-Nitrophenyl β-D-glucopyranoside), following redox reactions of the product (p-nitrophenol (pNP)). It is assumed that the initial reaction is the irreversible reduction of pNP to p-hydroxylaminophenol. Next, it can be either oxidized to p-nitrosophenol or dehydrated and oxidized to aminophenol. Both last reactions are reversible and can be used for redox cycling. The dual-electrode redox-cycling electrochemical signal was an order of magnitude larger than that of conventional single-working electrode transducers. A simple model for the gain is presented, predicting that an even larger gain is possible for sub-micron electrodes. In summary, this work demonstrates, for the first time, a redox cycling-based in vivo plant sensor, where diffusion-based amplification occurs inside a tobacco plant’s tissue. The technique can be applied to other plants as well as to medical and environmental monitoring systems.
Unilaterally Applied Resistance to Swing Leg Shows a Different Adaptation Pattern Compared to Split-Belt Treadmill in Patients with Stroke
Authors: Mizrachi Nama , Bar-Haim Simona , Treger Iuly , Melzer Itshak
Published: February 2023

Persons with chronic stroke (PwCS) have a decreased ability to ambulate and walk independently. We aimed to investigate the differences between the motor adaptation process for two different perturbation methods: split-belt treadmill walking and unilaterally applied resistance to the swing leg during walking. Twenty-two PwCS undergo split-belt treadmill walking and unilaterally applied resistance to the swing leg during walking, each one week apart. The test included three phases: the baseline period, the early-adaptation period and the late-adaptation period, as well as the early-de-adaptation period and the late-de-adaptation period. The average step length, swing duration, double-limb support duration, and coefficient of variance (CV) of these parameters were measured. During the split-belt treadmill walking, PwCS showed an adaptation of double-limb support duration symmetry (p = 0.004), specifically a trend between baseline versus early-adaptation (p = 0.07) and an after-effect (late-adaptation compare to early-de-adaptation, p = 0.09). In unilaterally applied resistance to the swing leg during walking, PwCS showed lower swing phase duration CV, in the adaptation period (baseline compare to adaptation, p = 0.006), and a trend toward increased variability of gait in the de-adaptation period compare to the adaptation periods (p = 0.099). The rate of adaptation and de-adaptation were alike between the two perturbation methods. Our findings show that the learning process happening in the central nervous system of PwCS may be dependent on the nature of the perturbation (mechanical resistance vs. split-belt) and that PwCS are able to adapt to two types of errors.
Effective Connectivity Evaluation of Resting-State Brain Networks in Alzheimer’s Disease, Amnestic Mild Cognitive Impairment, and Normal Aging: An Exploratory Study
Authors: Mohammadian Fatemeh , Noroozian Maryam , Sadeghi Arash Zare , Malekian Vahid , Saffar Azam , Talebi Mahsa , Hashemi Hasan , Mobarak Salari Hanieh , Samadi Fardin , Sodaei Forough , Rad Hamidreza Saligheh
Published: February 2023

(1) Background: Alzheimer’s disease (AD) is a neurodegenerative disease with a high prevalence. Despite the cognitive tests to diagnose AD, there are pitfalls in early diagnosis. Brain deposition of pathological markers of AD can affect the direction and intensity of the signaling. The study of effective connectivity allows the evaluation of intensity flow and signaling pathways in functional regions, even in the early stage, known as amnestic mild cognitive impairment (aMCI). (2) Methods: 16 aMCI, 13 AD, and 14 normal subjects were scanned using resting-state fMRI and T1-weighted protocols. After data pre-processing, the signal of the predefined nodes was extracted, and spectral dynamic causal modeling analysis (spDCM) was constructed. Afterward, the mean and standard deviation of the Jacobin matrix of each subject describing effective connectivity was calculated and compared. (3) Results: The maps of effective connectivity in the brain networks of the three groups were different, and the direction and strength of the causal effect with the progression of the disease showed substantial changes. (4) Conclusions: Impaired information flow in the resting-state networks of the aMCI and AD groups was found versus normal groups. Effective connectivity can serve as a potential marker of Alzheimer’s pathophysiology, even in the early stages of the disease.
A Correlation Analysis between Undergraduate Students’ Safety Behaviors in the Laboratory and Their Learning Efficiencies
Authors: Yu Deng-Guang , Du Yutong , Chen Jiahua , Song Wenliang , Zhou Tao
Published: February 2023

Students’ behaviors have a close relationship with their learning efficiencies, particularly about professional knowledge. Different types of behaviors should have different influences. Disclosing the special relationship between undergraduate students’ conscious safety behaviors in their laboratory experiments with their learning efficiencies is important for fostering them into professional talents. In this study, a course entitled “Advanced Methods of Materials Characterization” was arranged to contain three sections: theoretical learning in the classroom, eight characterization experiments in the laboratory in sequence, and self-training to apply the knowledge. In the final examination, eighteen percent was allocated to the examination questions about safety issues. The students’ scores for this section were associated with their total roll scores. Two quantitative relationships are disclosed. One is between the students’ final examination score (y) and their subjective consciousness of safety behaviors (x) in their laboratory experiments, as y = 5.56 + 4.83 x (R = 0.9192). The other is between their grade point average (y) and safety behavior evaluation (x) as y = 0.51 + 0.15 x (R = 0.7296). Undergraduate students’ behaviors in scientific laboratories need to be verified to have a close and positive relationship with their professional knowledge learning efficiencies. This offers a hint that improving students’ safety behaviors and enhancing their subjective safety awareness are conducive to improving their learning efficiency for professional knowledge.
The Effects of Different Exercise Approaches on Attention Deficit Hyperactivity Disorder in Adults: A Randomised Controlled Trial
Authors: Dinu Larisa M. , Singh Samriddhi N. , Baker Neo S. , Georgescu Alexandra L. , Singer Bryan F. , Overton Paul G. , Dommett Eleanor J.
Published: February 2023

Attention deficit hyperactivity disorder (ADHD) results in significant functional impairment. Current treatments, particularly for adults, are limited. Previous research indicates that exercise may offer an alternative approach to managing ADHD, but research into different types of exercise and adult populations is limited. The aim of this study was to examine the effects of acute exercise (aerobic cycling vs mind-body yoga exercises) on symptoms of ADHD in adults. Adults with ADHD (N = 82) and controls (N = 77) were randomly allocated to 10 min of aerobic (cycling) or mind-body (Hatha yoga) exercise. Immediately before and after exercise, participants completed the Test of Variables of Attention task, Delay Discounting Task, and Iowa Gambling Task to measure attention and impulsivity. Actigraphy measured movement frequency and intensity. Both groups showed improved temporal impulsivity post-exercise, with cycling beneficial to all, whilst yoga only benefited those with ADHD. There were no effects of exercise on attention, cognitive or motor impulsivity, or movement in those with ADHD. Exercise reduced attention and increased movement in controls. Exercise can improve temporal impulsivity in adult ADHD but did not improve other symptoms and worsened some aspects of performance in controls. Exercise interventions should be further investigated.
The Mediating Role of Employee Engagement in the Relationship between Flexible Work Arrangements and Turnover Intentions among Highly Educated Employees in the Republic of Serbia
Authors: Gašić Dimitrije , Berber Nemanja
Published: February 2023

The main objective of this research was to investigate the relationship between flexible working arrangements (FWA) and turnover intentions (TI), as well as the mediation effect of employee engagement (EE) in the relationship between flexible working arrangements and turnover intentions. The main research question is: what is the nature of the effect of flexible working arrangements on employees’ turnover intention, and the role of employee engagement in this relationship? The methodology of the paper consists of theoretical (literature review) and empirical parts (field research). The empirical research was performed on a sample of 514 highly educated employees from service sector organizations that operate in the Republic of Serbia. Sample collection lasted from January to October 2022, via Google Forms. The proposed relationships were tested by using the PLS-SEM method, with the application of the SmartPLS software. The main findings of the research are that there are direct positive effects of flexible work arrangements and employee engagement on turnover intentions, and that employee engagement has an indirect effect on the relationship between flexible work arrangements and turnover intentions. A partial mediation was found. Employees who are offered flexible work arrangements decrease their turnover intentions when they are more engaged at work.
Adaptability of the Sense of Agency in Healthy Young Adults in Sensorimotor Tasks for a Short Term
Authors: Mishima Mizuho , Hayashida Kazuki , Fukasaku Yoshiki , Ogata Rento , Ohsawa Kazuki , Iwai Ken , Wen Wen , Morioka Shu
Published: February 2023

Sense of agency (SoA) refers to the subjective feeling of controlling one’s own actions and sensory feedback. The SoA occurs when the predicted feedback matches the actual sensory feedback and is responsible for maintaining behavioral comfort. However, sensorimotor deficits because of illness cause incongruence between prediction and feedback, so the patient loses comfort during actions. Discomfort with actions associated with incongruence may continue robustly (i.e., “not” adaptable) throughout life because of the aftereffects of the disease. However, it is unclear how the SoA modulates when incongruency is experienced, even for a short term. The purpose of this study was to investigate the adaptability of the SoA in healthy participants in sensorimotor tasks for a short term. Participants were divided into congruent and incongruent exposure groups. The experimental task of manipulating the ratio of the self-control of a PC cursor was used to measure the SoA before and after exposure to congruent or incongruent stimuli. The results showed no significant differences between the groups before and after exposure for a short term. The finding that the SoA was not adaptable may assist in guiding the direction of future studies on how to correct incongruence.
Disturbing Values: Historic Thematic Framework as a Tool to Deal with the Soviet Architectural Legacy
Authors: Petrulis Vaidas , Doğan Huriye Armağan , Bliūdžius Raimondas
Published: February 2023

The value of cultural heritage is a constantly evolving and debated issue in heritage theory. While primarily the focus was on tangible qualities, intangible values gained more importance in the twenty-first century. However, in some cases, it can be complicated to evaluate the intangible values of specific artefacts with politically disturbing associations, such as Soviet architecture in a post-Soviet context. Based on a Lithuanian case study, the paper contributes to the debate on the value of Soviet architecture, focusing not on individual buildings but on broader themes. By using framework analysis as a conceptual background, the article analyzes the legacy of the Soviet past not as physical objects but as cultural references. Interpreting “cultural references” as containers of social and cultural meanings that are much broader than just the embodiment of the ideological system, the paper suggests that dissonance should not lead to the uncompromising delisting and probably the destruction of the built heritage of troublesome past. Although the article focuses on the evaluation of Soviet architecture in a post-Soviet context, the conceptual approach can contribute to the broader debate on postcolonial heritage globally.
A Beam Test Study on the Bond Performance between Epoxy-Coated Reinforcement and Geopolymer Concrete
Authors: Ma Zhangyong , Yue Qingrui , Chen Zhihua
Published: February 2023

An epoxy-coated reinforcement geopolymer concrete structure with good durability and energy-saving properties can be formed by combining epoxy-coated reinforcement and geopolymer concrete. The bond strength is the precondition for the two to work together. In this paper, 13 beam specimens (11 epoxy-coated reinforcements and 2 ordinary deformed reinforcements) were designed to investigate the influence of the strength of geopolymer concrete, diameter of the reinforcement, bonding length and type of reinforcement on the bond performance between reinforcement and geopolymer concrete. The test results show that the ultimate bond strength of the epoxy-coated reinforcement (ECR) and geopolymer concrete decreased by 7.32% and 14.76%, respectively, when the rebar diameter increased from 14 mm to 16 mm and then to 20 mm. The ultimate bond strength between ordinary threaded reinforcement and geopolymer concrete was slightly higher than that between ECR and geopolymer concrete. When the length of the bond section is small or the concrete strength is low, the beam specimen is prone to the failure of the reinforcement pullout. The specimen with the larger reinforcement diameter is prone to concrete splitting failure. However, the specimens with medium bond length and small reinforcement diameter suffered from pull-out failure after concrete splitting. In this paper, based on the test data, the bond-slip constitutive model of ECR and geopolymer concrete was established, and the bond-slip curve obtained by this model was in good agreement with the measured curve. In addition, the calculation formula of the ultimate bond strength between ECR and geopolymer concrete was also proposed in this paper, which can provide theoretical reference for the engineering application of geopolymer concrete.
Experimental Investigation of Pounding Responses in Base-Isolated Frame Structures at Expansion Gap
Authors: Hubballi Siddalingappa S. , Jangid R. S.
Published: February 2023

Experimental simulation and parametric studies have been conducted to investigate the effect of pounding in base-isolated (BI) frame structures with expansion gaps. Earthquakes often result in extensive damage at expansion joints, which encouraged this experimental evaluation of pounding between BI frames. In the present research, the pounding effect in a series of combinations, such as adjacent flexible and stiffer BI frames of similar frequency and different frequency to adjacent frames, were experimentally analysed to find better combinations of adjacent structures with an expansion gap. In the experiments, the surfaces at the isolation and frame slab levels were free to impact in order to investigate direct pounding (DP) and mitigated pounding (MP) by inserting a neoprene rubber pad into the gap. The investigation was conducted by comparing BI structures with and without pounding responses as a result of the El Centro earthquake excitation. To demonstrate the extent of the pounding effect in adjacent structures, the horizontal and pounding force variations in each combination during DP and MP were evaluated. The experimental responses analysis results help in justifying a better variety of adjacent BI structures with an expansion gap. This experimental study shows that mitigating material in the gap is more efficient for nearer frequency combinations than for different frequency frame combinations.
Hydrogen Production by N-Heterocycle Dehydrogenation over Pd Supported on Aerogel-Prepared Mg-Al Oxides
Authors: Shivtsov Danil M. , Koskin Anton P. , Stepanenko Sergey A. , Ilyina Ekaterina V. , Ayupov Artem B. , Bedilo Alexander F. , Yakovlev Vadim A.
Published: February 2023

Tetradecahydrophenazine (14HP) is a nitrogen-containing heterocycle compound with a high content of hydrogen that can be released during its dehydrogenation to phenazine (P). The high stability of the 14HP/P pair and relatively low dehydrogenation temperature make 14HP a promising organic hydrogen carrier. This manuscript is devoted to the investigation of hydrogen production by 14HP dehydrogenation over Pd supported on a series of magnesium-aluminum oxides prepared by the aerogel method. This technique made it possible to synthesize catalyst supports characterized by a high surface area and high concentration of surface active sites where active transition metals could be stabilized in a finely dispersed state. The synthesized aerogels had high specific surface areas and pore volumes. A surface area as high as 600 m2/g after calcination at 500 °C was observed for the mixed aerogel with an Mg:Al ratio of 1:4. An increase in the concentration of acidic electron-acceptor sites determined by EPR on the surface of the mixed magnesium-aluminum oxide supports with a high surface area prepared by the aerogel method was found to result in higher hydrogen production due to the faster dehydrogenation of sterically hindered nitrogen-containing tetradecahydrophenazine heterocycles.
Complete Elimination of the Ciprofloxacin Antibiotic from Water by the Combination of Adsorption–Photocatalysis Process Using Natural Hydroxyapatite and TiO2
Authors: Cheikh Sabrina , Imessaoudene Ali , Bollinger Jean-Claude , Hadadi Amina , Manseri Amar , Bouzaza Abdelkrim , Assadi Aymen , Amrane Abdeltif , Zamouche Meriem , El Jery Atef , Mouni Lotfi
Published: February 2023

The main objective of this work was to assess the performance of combined processes, adsorption/ photodegradation of the ciprofloxacin antibiotic (CIP). Adsorption was achieved on natural hydroxyapatite (nat-HA) in the batch mode. The effect of pH (3–12), initial ciprofloxacin concentration (C0, 25–200 mg L−1), adsorbent dose (m, 0.25–3 g L−1), and temperature (T, 298–328 K) on the ciprofloxacin adsorption capacity was studied. At 298 K, the maximum uptake of 147.7 mg g−1 was observed with pH close to 8, 1 g L−1nat-HA dose, and 150 mg L−1 initial CIP concentration. Adsorption was effective, with a removal percentage of 82% within 90 minutes of contact time. For ciprofloxacin adsorption onto nat-HA, a pseudo-second-order kinetic model is well-suited. The Langmuir isotherm model successfully fit the experimental data and the process was spontaneous and exothermic. The coupling processes (adsorption/photocatalysis) were examined and found to be highly effective. For the remaining concentrations, the maximum degradation efficiency and mineralization yield were ~100% and 98.5%, respectively, for 1 mg L−1 initial CIP. The combination of the strong adsorption capacity of natural hydroxyapatite and the high photocatalytic activity of TiO2 can be an effective technique for removing fluoroquinolone antibiotics from wastewater.
A Comparison of the Sensing Behavior for Pt-Mo/C-, Pt-Zr/C-, Pt-Fe-Ir/C-, and Pt/C-Modified Glassy Carbon Electrodes for the Oxidation of Ascorbic Acid and Dopamine
Authors: Weng Yu-Ching , Su-Chen Jia-Yi , Yang Ting-Yu , Chiang Chieh-Lin
Published: February 2023

This study compares the sensing performance for platinum-molybdenum-, platinum-zirconium-, platinum-iron-iridium-, and platinum-modified electrodes in terms of the amperometric detection of ascorbic acid (AA) and dopamine (DA). The Pt, Pt-Mo, Pt-Zr, and Pt-Fe-Ir electrocatalysts are fabricated by chemical reduction on a carbon black support (XC-72) and are further modified on a glassy carbon electrode (GCE) as sensing electrodes. The Pt-Mo/C/GCE exhibits better electrocatalytic activity toward AA and DA than the Pt/C/GCE, Pt-Zr/C/GCE, and Pt-Fe-Ir/C/GCE. The Pt-Mo/C/GCE exhibits a sensitivity of 31.29 µA mM−1 to AA at 0.3 V vs. Ag/AgCl and a sensitivity of 72.24 µA mM−1 to DA at 0.6 V vs. Ag/AgCl and is reproducible and stable. This electrode has a respective limit of detection of 7.69 and 6.14 µM for AA and DA. Sucrose, citric acid, tartaric acid, and uric acid do not interfere with AA and DA detection. The diffusion coefficient and kinetic parameters, such as the catalytic rate constant and the heterogeneous rate constant for AA and DA, are determined using electrochemical approaches.
Application of Heterogeneous Catalytic Ozonation in Wastewater Treatment: An Overview
Authors: Li Xingxing , Fu Li , Chen Fei , Zhao Shichao , Zhu Jiangwei , Yin Chengliang
Published: February 2023

Catalytic ozonation is a non-selective mineralization technology of organic matter in water by using active free radicals generated by ozone degradation. Catalytic ozonation technology can be divided into homogeneous catalytic reactions using metal ions as catalysts and heterogeneous catalytic reactions using solid catalysts. Homogeneous catalytic ozonation technology has many problems, such as low mineralization rate, secondary pollution caused by the introduction of metal ions and low utilization efficiency of oxidants, which limit its practical application. Compared with homogeneous catalytic ozonation technology, heterogeneous catalytic ozonation technology has the advantages of easy recovery, lower cost of water treatment, higher activity and improved mineralization rate of organic matter. This overview classifies and describes catalysts for heterogeneous catalytic ozonation technology, including the different types of metal oxides, metal-free catalysts, and substrates used to immobilize catalysts. In addition, the heterogeneous catalytic ozonation process involved in the multiphase complex reaction process is discussed. The effects of different parameters on the performance of heterogeneous catalytic ozonation are also discussed.
Catalytic Transformation of Biomass-Derived Hemicellulose Sugars by the One-Pot Method into Oxalic, Lactic, and Levulinic Acids Using a Homogeneous H2SO4 Catalyst
Authors: Sobuś Natalia , Czekaj Izabela
Published: February 2023

This article presents the conditions for the conversion of hemicellulose with different contents of C6 and C5 carbohydrates and uronic acids based on the OrganoCat process, and the abbreviations M1, M2, and M3 are used. Homogenous catalysis with sulfuric acid (VI) in the concentration range of 0.1–1 M was used in the study to determine its activity on the ability to transform a hemicellulose mixture. The process was carried out using the one-pot technique in the temperature range of 100–250 °C for 1–5 h. Based on the use of the chromatographic technique (HPLC-RID) together with a comparison with standard substances, the resulting chemical compounds were determined and identified from the post-reaction mixtures. The degree of covalence of the raw material, the selectivity of the obtained chemical compounds, and the yield of lactic acid were also determined. Based on the obtained results, lactic acid with the highest yield (64.57%) was obtained after 1 h of the process from the M1 mixture at the temperature of 100 °C with 0.1 M sulfuric acid (VI) as a catalyst. The formation of oxalic acid was also observed, which is present in all post-reaction mixtures, regardless of the composition of the raw material, temperature, and time. Its efficiency was determined at an average level of 90%.
Bimetallic Metal-Organic Framework Derived Nanocatalyst for CO2 Fixation through Benzimidazole Formation and Methanation of CO2
Authors: Helal Aasif , Sanhoob Mohammed Ahmed , Hoque Bosirul , Usman Muhammad , Zahir Md. Hasan
Published: February 2023

In this paper, a bimetallic Metal-Organic Framework (MOF) CoNiBTC was employed as a precursor for the fabrication of bimetallic nanoalloys CoNi@C evenly disseminated in carbon shells. These functional nanomaterials are characterized by powdered X-ray diffraction (PXRD), Fourier Transform Infra-Red spectroscopy (FTIR), surface area porosity analyzer, X-ray photoelectron spectroscopy (XPS), Field emission scanning electron microscopy (FESEM), Transmission electron microscopy (TEM), Hydrogen Temperature-Programmed Reduction (H2 TPR), CO2 Temperature-Programmed Desorption (CO2-TPD), and Inductively Coupled Plasma Mass Spectrometry (ICP-MS). This nanocatalyst was utilized in the synthesis of benzimidazole from o-phenylenediamine in the presence of CO2 and H2 in a good yield of 81%. The catalyst was also efficient in the manufacture of several substituted benzimidazoles with high yield. Due to the existence of a bimetallic nanoalloy of Co and Ni, this catalyst was also employed in the methanation of CO2 with high selectivity (99.7%).
The Absence of FAIM Leads to a Delay in Dark Adaptation and Hampers Arrestin-1 Translocation upon Light Reception in the Retina
Authors: Sirés Anna , Pazo-González Mateo , López-Soriano Joaquín , Méndez Ana , de la Rosa Enrique J. , de la Villa Pedro , Comella Joan X. , Hernández-Sánchez Catalina , Solé Montse
Published: February 2023

The short and long isoforms of FAIM (FAIM-S and FAIM-L) hold important functions in the central nervous system, and their expression levels are specifically enriched in the retina. We previously described that Faim knockout (KO) mice present structural and molecular alterations in the retina compatible with a neurodegenerative phenotype. Here, we aimed to study Faim KO retinal functions and molecular mechanisms leading to its alterations. Electroretinographic recordings showed that aged Faim KO mice present functional loss of rod photoreceptor and ganglion cells. Additionally, we found a significant delay in dark adaptation from early adult ages. This functional deficit is exacerbated by luminic stress, which also caused histopathological alterations. Interestingly, Faim KO mice present abnormal Arrestin-1 redistribution upon light reception, and we show that Arrestin-1 is ubiquitinated, a process that is abrogated by either FAIM-S or FAIM-L in vitro. Our results suggest that FAIM assists Arrestin-1 light-dependent translocation by a process that likely involves ubiquitination. In the absence of FAIM, this impairment could be the cause of dark adaptation delay and increased light sensitivity. Multiple retinal diseases are linked to deficits in photoresponse termination, and hence, investigating the role of FAIM could shed light onto the underlying mechanisms of their pathophysiology.
Understanding Intra- and Inter-Species Variability in Neural Stem Cells’ Biology Is Key to Their Successful Cryopreservation, Culture, and Propagation
Authors: Radoszkiewicz Klaudia , Jezierska-Woźniak Katarzyna , Waśniewski Tomasz , Sarnowska Anna
Published: February 2023

Although clinical trials on human neural stem cells (hNSCs) have already been implemented in the treatment of neurological diseases and they have demonstrated their therapeutic effects, many questions remain in the field of preclinical research regarding the biology of these cells, their therapeutic properties, and their neurorestorative potential. Unfortunately, scientific reports are inconsistent and much of the NSCs research has been conducted on rodents rather than human cells for ethical reasons or due to insufficient cell material. Therefore, a question arises as to whether or which conclusions drawn on the isolation, cell survival, proliferation, or cell fate observed in vitro in rodent NSCs can be introduced into clinical applications. This paper presents the effects of different spatial, nutritional, and dissociation conditions on NSCs’ functional properties, which are highly species-dependent. Our study confirmed that the discrepancies in the available literature on NSCs survival, proliferation, and fate did not only depend on intra-species factors and applied environmental conditions, but they were also affected by significant inter-species variability. Human and rodent NSCs share one feature, i.e., the necessity to be cultured immediately after isolation, which significantly maintains their survival. Additionally, in the absence of experiments on human cells, rat NSCs biology (neurosphere formation potential and neural differentiation stage) seems closer to that of humans rather than mice in response to environmental factors.
Prothymosin α Plays Role as a Brain Guardian through Ecto-F1 ATPase-P2Y12 Complex and TLR4/MD2
Authors: Ueda Hiroshi
Published: February 2023

Prothymosin alpha (ProTα) was discovered to be a necrosis inhibitor from the conditioned medium of a primary culture of rat cortical neurons under starved conditions. This protein carries out a neuronal cell-death-mode switch from necrosis to apoptosis, which is, in turn, suppressed by a variety of neurotrophic factors (NTFs). This type of NTF-assisted survival action of ProTα is reproduced in cerebral and retinal ischemia–reperfusion models. Further studies that used a retinal ischemia–reperfusion model revealed that ProTα protects retinal cells via ecto-F1 ATPase coupled with the Gi-coupled P2Y12 receptor and Toll-like receptor 4 (TLR4)/MD2 coupled with a Toll–IL-1 receptor domain-containing adaptor inducing IFN-β (TRIF). In cerebral ischemia–reperfusion models, ProTα has additional survival mechanisms via an inhibition of matrix metalloproteases in microglia and vascular endothelial cells. Heterozygous or conditional ProTα knockout mice show phenotypes of anxiety, memory learning impairment, and a loss of neurogenesis. There are many reports that ProTα has multiple intracellular functions for cell survival and proliferation through a variety of protein–protein interactions. Overall, it is suggested that ProTα plays a key role as a brain guardian against ischemia stress through a cell-death-mode switch assisted by NTFs and a role of neurogenesis.
C/EBPβ Coupled with E2F2 Promoted the Proliferation of hESC-Derived Hepatocytes through Direct Binding to the Promoter Regions of Cell-Cycle-Related Genes
Authors: Liu Shoupei , Wang Jue , Chen Sen , Han Zonglin , Wu Haibin , Chen Honglin , Duan Yuyou
Published: February 2023

Human embryonic stem cells (hESCs) hold the potential to solve the problem of the shortage of functional hepatocytes in clinical applications and drug development. However, a large number of usable hepatocytes derived from hESCs cannot be effectively obtained due to the limited proliferation capacity. In this study, we found that enhancement of liver transcription factor C/EBPβ during hepatic differentiation could not only significantly promote the expression of hepatic genes, such as albumin, alpha fetoprotein, and alpha-1 antitrypsin, but also dramatically reinforce proliferation-related phenotypes, including increasing the expression of proliferative genes, such as CDC25C, CDC45L, and PCNA, and the activation of cell cycle and DNA replication pathways. In addition, the analysis of CUT&Tag sequencing further revealed that C/EBPβ is directly bound to the promoter region of proliferating genes to promote cell proliferation; this interaction between C/EBPβ and DNA sequences of the promoters was verified by luciferase assay. On the contrary, the knockdown of C/EBPβ could significantly inhibit the expression of the aforementioned proliferative genes. RNA transcriptome analysis and GSEA enrichment indicated that the E2F family was enriched, and the expression of E2F2 was changed with the overexpression or knockdown of C/EBPβ. Moreover, the results of CUT&Tag sequencing showed that C/EBPβ also directly bound the promoter of E2F2, regulating E2F2 expression. Interestingly, Co-IP analysis exhibited a direct binding between C/EBPβ and E2F2 proteins, and this interaction between these two proteins was also verified in the LO2 cell line, a hepatic progenitor cell line. Thus, our results demonstrated that C/EBPβ first initiated E2F2 expression and then coupled with E2F2 to regulate the expression of proliferative genes in hepatocytes during the differentiation of hESCs. Therefore, our findings open a new avenue to provide an in vitro efficient approach to generate proliferative hepatocytes to potentially meet the demands for use in cell-based therapeutics as well as for pharmaceutical and toxicological studies.
B Cell Signaling and Activation in Autoimmunity
Authors: Hendriks Rudi W. , Corneth Odilia B. J.
Published: February 2023

Autoreactive B cells play a key role in the initiation or aggravation of many systemic and tissue-specific autoimmune disorders [...]
Increased Nuclear FOXP2 Is Related to Reduced Neural Stem Cell Number and Increased Neurogenesis in the Dorsal Telencephalon of Embryos of Diabetic Rats through Histamine H1 Receptors
Authors: De la Merced-García Diana Sarahi , Sánchez-Barrera Ángel , Hernández-Yonca Juan , Mancilla Ismael , García-López Guadalupe , Díaz Néstor Fabián , Terrazas Luis Ignacio , Molina-Hernández Anayansi
Published: February 2023

Diabetic rat embryos have increased cortical neurogenesis and neuron maturation, and their offspring presented altered neuron polarity, lamination, and diminished neuron excitability. The FOXP2 overexpression results in higher cortical neurogenesis by increasing the transition of radial glia to the intermediate progenitor. Similarly, histamine through H1-receptor activation increases cortical neuron differentiation. Indeed, blocking the H1-receptor by the systemic administration of chlorpheniramine to diabetic pregnant rats prevents increased neurogenesis. Here, we explore the relationship between the H1-receptor and FOXP2 on embryo neurogenesis from diabetic dams. Through qRT-PCR, Western blot, immunohistofluorescence, and flow cytometry, we showed an increased FOXP2 expression and nuclear localization, a reduced Nestin expression and -positive cells number, and a higher PKCα expression in the cortical neuroepithelium of fourteen-day-old embryos from diabetic rats. Interestingly, this scenario was prevented by the chlorpheniramine systemic administration to diabetic pregnant rats at embryo day twelve. These data, together with the bioinformatic analysis, suggest that higher H1-receptor activity in embryos under high glucose increases FOXP2 nuclear translocation, presumably through PKCα phosphorylation, impairing the transition of radial glia to intermediate progenitor and increasing neuron differentiation in embryos of diabetic rats.
Endothelial Specific Deletion of Autotaxin Improves Stroke Outcomes
Authors: Bhattarai Susmita , Subedi Utsab , Manikandan Shrivats , Sharma Sudha , Sharma Papori , Miller Chloe , Bhuiyan Md Shenuarin , Kidambi Srivatsan , Aidinis Vassilis , Sun Hong , Miriyala Sumitra , Panchatcharam Manikandan
Published: February 2023

Autotaxin (ATX) is an extracellular secretory enzyme (lysophospholipase D) that catalyzes the hydrolysis of lysophosphatidyl choline to lysophosphatidic acid (LPA). The ATX–LPA axis is a well-known pathological mediator of liver fibrosis, metastasis in cancer, pulmonary fibrosis, atherosclerosis, and neurodegenerative diseases. Additionally, it is believed that LPA may cause vascular permeability. In ischemic stroke, vascular permeability leading to hemorrhagic transformation is a major limitation for therapies and an obstacle to stroke management. Therefore, in this study, we generated an endothelial-specific ATX deletion in mice (ERT2 ATX−/−) to observe stroke outcomes in a mouse stroke model to analyze the role of endothelial ATX. The AR2 probe and Evans Blue staining were used to perform the ATX activity and vascular permeability assays, respectively. Laser speckle imaging was used to observe the cerebral blood flow following stroke. In this study, we observed that stroke outcomes were alleviated with the endothelial deletion of ATX. Permeability and infarct volume were reduced in ERT2 ATX−/− mice compared to ischemia–reperfusion (I/R)-only mice. In addition, the cerebral blood flow was retained in ERT2 ATX−/− compared to I/R mice. The outcomes in the stroke model are alleviated due to the limited LPA concentration, reduced ATX concentration, and ATX activity in ERT2 ATX−/− mice. This study suggests that endothelial-specific ATX leads to increased LPA in the brain vasculature following ischemic–reperfusion and ultimately disrupts vascular permeability, resulting in adverse stroke outcomes.
Pharmacological Targeting of the RAGE-NFκB Signalling Axis Impedes Monocyte Activation under Diabetic Conditions through the Repression of SHP-2 Tyrosine Phosphatase Function
Authors: Dorenkamp Marc , Nasiry Madina , Semo Dilvin , Koch Sybille , Löffler Ivonne , Wolf Gunter , Reinecke Holger , Godfrey Rinesh
Published: February 2023

Monocytes play a vital role in the development of cardiovascular diseases. Type 2 diabetes mellitus (T2DM) is a major CVD risk factor, and T2DM-induced aberrant activation and enhanced migration of monocytes is a vital pathomechanism that leads to atherogenesis. We recently reported the upregulation of SHP-2 phosphatase expression in mediating the VEGF resistance of T2DM patient-derived monocytes or methylglyoxal- (MG, a glucose metabolite and advanced glycation end product (AGE) precursor) treated monocytes. However, the exact mechanisms leading to SHP-2 upregulation in hyperglycemic monocytes are unknown. Since inflammation and accumulation of AGEs is a hallmark of T2DM, we hypothesise that inflammation and AGE-RAGE (Receptor-for-AGEs) signalling drive SHP-2 expression in monocytes and blockade of these pathways will repress SHP-2 function. Indeed, monocytes from T2DM patients revealed an elevated SHP-2 expression. Under normoglycemic conditions, the serum from T2DM patients strongly induced SHP-2 expression, indicating that the T2DM serum contains critical factors that directly regulate SHP-2 expression. Activation of pro-inflammatory TNFα signalling cascade drove SHP-2 expression in monocytes. In line with this, linear regression analysis revealed a significant positive correlation between TNFα expression and SHP-2 transcript levels in T2DM monocytes. Monocytes exposed to MG or AGE mimetic AGE-BSA, revealed an elevated SHP-2 expression and co-treatment with an NFκB inhibitor or genetic inhibition of p65 reversed it. The pharmacological inhibition of RAGE was sufficient to block MG- or AGE-BSA-induced SHP-2 expression and activity. Confirming the importance of RAGE-NFκB signalling in regulating SHP-2 expression, the elevated binding of NFκB to the SHP-2 promoter—induced by MG or AGE-BSA—was reversed by RAGE and NFκB inhibition. Besides, we detected elevated RAGE levels in human and murine T2DM monocytes and monocytes exposed to MG or AGE-BSA. Importantly, MG and AGE-BSA treatment of non-T2DM monocytes phenocopied the aberrant pro-migratory phenotype of T2DM monocytes, which was reversed entirely by either SHP-2- or RAGE inhibition. In conclusion, these findings suggest a new therapeutic approach to prevent accelerated atherosclerosis in T2DM patients since inhibiting the RAGE-NFκB-SHP-2 axis impeded the T2DM-driven, SHP-2-dependent monocyte activation.
Cell Surface Charge Mapping Using a Microelectrode Array on ITO Substrate
Authors: Ouyang Leixin , Shaik Rubia , Xu Ruiting , Zhang Ge , Zhe Jiang
Published: February 2023

Many cellular functions are regulated by cell surface charges, such as intercellular signaling and metabolism. Noninvasive measurement of surface charge distribution of a single cell plays a vital role in understanding cellular functions via cell membranes. We report a method for cell surface charge mapping via photoelectric interactions. A cell is placed on an array of microelectrodes fabricated on a transparent ITO (indium tin oxide) surface. An incident light irradiates the ITO surface from the backside. Because of the influence of the cell surface charge (or zeta potential), the photocurrent and the absorption of the incident light are changed, inducing a magnitude change of the reflected light. Hence, the cell surface charge distribution can be quantified by analyzing the reflected light intensity. This method does not need physical or chemical modification of the cell surface. We validated this method using charged microparticles (MPs) and two types of cells, i.e., human dermal fibroblast cells (HDFs) and human mesenchymal stem cells (hMSC). The measured average zeta potentials were in good agreement with the standard electrophoresis light scattering method.
An Update on Phytochemicals in Redox Homeostasis: “Virtuous or Evil” in Cancer Chemoprevention?
Authors: Fatma Homa , Jameel Mohd , Siddique Hifzur R.
Published: February 2023

Redox homeostasis, a dynamic process ensuring a balance between cellular oxidizing and reducing reactions, is crucial for maintaining healthy cellular physiology and regulating many biological processes, requiring continuous monitoring and fine-tuning. Reactive species play a critical role in intra/intercellular signaling, and each cell has a specific system guarding cellular redox homeostasis. ROS signaling and oxidative stress are involved in cancer initiation and progression. However, the generation of reactive species beyond the threshold level inside the tumor microenvironment is considered one of the therapeutic approaches. Various studies have shown that some phytochemicals can target the redox homeostasis of the tumor microenvironment. Recent advances have focused on developing and introducing phytochemical interventions as favorable therapeutic options against cancer. However, studies have also suggested the “virtuous” and “evil” impacts of phytochemicals. Some phytochemicals enhance therapeutic efficacy by promoting intracellular oxidant accumulation. However, under certain conditions, some phytochemicals may harm the cellular microenvironment to promote cancer and tend to target different pathways for cancer initiation and development instead of targeting redox homeostasis. In this context, this review is focused on providing an overall understanding of redox homeostasis and intends to highlight the potential positive and negative impacts of phytochemicals in redox homeostasis and disease development. We also discuss the recent nanotechnology-based advancements in combating cancer development.
Tunable Nanoplasmonic Transducers: Performance Analysis and Potential Application
Authors: Colombelli Adriano , Lospinoso Daniela , Rella Roberto , Manera Maria Grazia
Published: February 2023

Plasmonic nanostructures with tunable optical properties can have many different applications, including high-sensitivity optical sensing for biological and chemical analyses in different field such as medical, environmental and food safety. The realization of an optimized sensing platform is closely related to the ability to finely control optical properties of nanostructures, which are, in turn, intimately linked to their geometrical and compositional characteristics. In this paper, an efficient and reproducible fabrication protocol, based on nanosphere lithography, for the realization of metal nanostructures with tunable plasmonic features is presented. In particular, the relationships between the geometric characteristics of different types of nanostructures with related optical phenomena such as enhanced absorption or extraordinary transmission are investigated in detail. These properties, together with electric field enhancement and confinement, are characterized and optimized in view of the employment of the fabricated nanostructures as optical transducers in nanoplasmonic chemosensor platforms working in the UV-VIS spectral range.
Effects of Sports, Exercise Training, and Physical Activity in Children with Congenital Heart Disease—A Review of the Published Evidence
Authors: Dold Simone K. , Haas Nikolaus A. , Apitz Christian
Published: February 2023

Children and adolescents with congenital heart disease (CHD) should be encouraged to adopt a physically active lifestyle, ideally by participating in sports activities at school and sports clubs. Children with complex CHD or other risk factors (for example, pacemakers, cardioverter-defibrillators, channelopathies) may, however, need specific individualized training programs. This review article summarizes the current knowledge regarding the clinical effects of sports and exercise training on CHD and its pathophysiologic mechanisms. An evidence-based approach based on a literature search, using PubMed, Medline, CINHAL, Embase, and the Cochrane Library was conducted, last completed on 30 December 2021. In studies with 3256 CHD patients in total, including 10 randomized controlled trials, 14 prospective interventional trials, 9 observational trials, and 2 surveys, exercise training has been shown to improve exercise capacity and physical activity, motoric skills, muscular function, and quality of life. Sports and exercise training appears to be effective and safe in CHD patients. Despite being cost-efficient, training programs are currently scarcely reimbursed; therefore, support from healthcare institutions, commissioners of healthcare, and research-funding institutions is desirable. There is a strong need to establish specialized rehabilitation programs for complex CHD patients to enhance these patients’ access to this treatment intervention. Further studies may be desirable to confirm these data to investigate the impact on risk profiles and to identify the most advantageous training methodology and underlying pathophysiological mechanisms.
Ozoile Reduces the LPS-Induced Inflammatory Response in Colonic Epithelial Cells and THP-1 Monocytes
Authors: Bertuccio Maria Paola , Rizzo Valentina , Arena Salvatore , Trainito Alessandra , Montalto Angela Simona , Caccamo Daniela , Currò Monica , Romeo Carmelo , Impellizzeri Pietro
Published: February 2023

Inappropriate activation of immune functions in intestinal epithelial cells can lead to inflammation that is characterized also by infiltration into intestinal tissue of monocytes/macrophages. Current therapies for intestinal inflammation include anti-inflammatory, immunosuppressive and biological drugs. Ozoile (stable ozonides) has been reported to exert anti-inflammatory effects. However, ozonated oil has been used mainly for topical applications and no data are available about its effects on intestinal cells or immune cells. In this study, we evaluated Ozoile effects on human HT-29 colonic cells and THP-1 monocytic cells stimulated with LPS to induce inflammation. HT-29 and THP-1 cells were treated with LPS in the presence/absence of Ozoile for 4 h. Biomarkers of inflammation, some members of tight junctions and the adhesion molecule ICAM were assessed by qRT-PCR. Protein expression was analyzed by Western blotting. The release of TNF-α and IL-1β was measured by ELISA. In HT-29, Ozoile inhibited LPS-induced expression of TNF-α, IL-1β, ZO-1, CLDN1, NOS2 and MMP-2 and increased the expression of Nrf2 and SOD2 antioxidant proteins. In THP-1 cells, the LPS induction of TNF-α, IL-1β and ICAM was counteracted by Ozoile treatment. Our in vitro results demonstrate the effectiveness of Ozoile in reducing the inflammatory response in intestinal and monocytic cells. Further in vivo studies are necessary to confirm its possible use for intestinal inflammatory conditions.