bluuebunny/crossref_metadata_2025 huggingface dataset:
Fluid Candidate Biomarkers for Alzheimer’s Disease: A Precision Medicine Approach
Authors: Del Prete Eleonora , Beatino Maria Francesca , Campese Nicole , Giampietri Linda , Siciliano Gabriele , Ceravolo Roberto , Baldacci Filippo
Published: November 2020

A plethora of dynamic pathophysiological mechanisms underpins highly heterogeneous phenotypes in the field of dementia, particularly in Alzheimer’s disease (AD). In such a faceted scenario, a biomarker-guided approach, through the implementation of specific fluid biomarkers individually reflecting distinct molecular pathways in the brain, may help establish a proper clinical diagnosis, even in its preclinical stages. Recently, ultrasensitive assays may detect different neurodegenerative mechanisms in blood earlier. ß-amyloid (Aß) peptides, phosphorylated-tau (p-tau), and neurofilament light chain (NFL) measured in blood are gaining momentum as candidate biomarkers for AD. P-tau is currently the more convincing plasma biomarker for the diagnostic workup of AD. The clinical role of plasma Aβ peptides should be better elucidated with further studies that also compare the accuracy of the different ultrasensitive techniques. Blood NFL is promising as a proxy of neurodegeneration process tout court. Protein misfolding amplification assays can accurately detect α-synuclein in cerebrospinal fluid (CSF), thus representing advancement in the pathologic stratification of AD. In CSF, neurogranin and YKL-40 are further candidate biomarkers tracking synaptic disruption and neuroinflammation, which are additional key pathophysiological pathways related to AD genesis. Advanced statistical analysis using clinical scores and biomarker data to bring together individuals with AD from large heterogeneous cohorts into consistent clusters may promote the discovery of pathophysiological causes and detection of tailored treatments.
The Effect of Glue Cohesive Stiffness on the Elastic Performance of Bent Wood–CFRP Beams
Authors: Kawecki Bartosz , Podgórski Jerzy
Published: November 2020

This paper presents experimental, theoretical and numerical studies of wood-CFRP beams bonded with polyurethane (PUR) adhesive. The analyses include two types of CFRP (carbon fibre-reinforced polymer) strengthening configurations and pure glue laminated timber beams as a reference. Through detailed analyses of a double-lap connection on blocks with and without CFRP strips, the authors state that neglecting the cohesive stiffness of adhesive layers may lead to an overestimation of an overall beam’s stiffness. This is significant with wood–CFRP connections, which showed values two times lower than with wood–wood connections. Theoretical modelling of the equivalent area used in a theory of composites provided much stiffer behaviour of the beams than in laboratory experiments. It proves that a PUR glue eliminates the possibility of using simple models that assume a perfect connection between bonded parts. These conclusions led the authors to use the finite element method (FEM) to take into account the cohesive stiffness. The FEM, based on the properties obtained from a double-lap joint analysis, allowed for the precise prediction of the elastic stiffness of the beams.
Comparison of Drying Shrinkage of Concrete Specimens Recycled Heavyweight Waste Glass and Steel Slag as Aggregate
Authors: Choi So Yeong , Kim Il Sun , Yang Eun Ik
Published: November 2020

This study analyzed the fundamental properties of concrete using steel slag, to test its viability as an aggregate material. An experimental investigation into the effect of steel slag as a coarse aggregate, and heavyweight waste glass as a fine aggregate, on the drying shrinkage of concrete was performed. The calculated shrinkage strain was compared to five different shrinkage prediction models, namely, the ACI 209, B3, KCI 2012, EC 2 and GL 2000 model codes, to evaluate their ability to accurately predict shrinkage behavior. From the results, the elastic modulus of concrete increased with the increase in steel slag substitution ratio, however drying shrinkage decreased. The predictive value of the existing prediction model of drying shrinkage differed from the experimental values, and requires correction to improve its accuracy. The B3 model code showed the best prediction results of drying shrinkage.
A Low-Cost Open Source Device for Cell Microencapsulation
Authors: Pereira Miriam Salles , Cardoso Liana Monteiro da Fonseca , da Silva Tatiane Barreto , Teixeira Ayla Josma , Mizrahi Saul Eliahú , Ferreira Gabriel Schonwandt Mendes , Dantas Fabio Moyses Lins , Cotta-de-Almeida Vinicius , Alves Luiz Anastacio
Published: November 2020

Microencapsulation is a widely studied cell therapy and tissue bioengineering technique, since it is capable of creating an immune-privileged site, protecting encapsulated cells from the host immune system. Several polymers have been tested, but sodium alginate is in widespread use for cell encapsulation applications, due to its low toxicity and easy manipulation. Different cell encapsulation methods have been described in the literature using pressure differences or electrostatic changes with high cost commercial devices (about 30,000 US dollars). Herein, a low-cost device (about 100 US dollars) that can be created by commercial syringes or 3D printer devices has been developed. The capsules, whose diameter is around 500 µm and can decrease or increase according to the pressure applied to the system, is able to maintain cells viable and functional. The hydrogel porosity of the capsule indicates that the immune system is not capable of destroying host cells, demonstrating that new studies can be developed for cell therapy at low cost with microencapsulation production. This device may aid pre-clinical and clinical projects in low- and middle-income countries and is lined up with open source equipment devices.
Evaluation of Self-Propelled Rotary Tool in the Machining of Hardened Steel Using Finite Element Models
Authors: Umer Usama , Kishawy Hossam , Abidi Mustufa Haider , Mian Syed Hammad , Moiduddin Khaja
Published: November 2020

This paper presents a model for assessing the performance of self-propelled rotary tool during the processing of hardened steel. A finite element (FE) model has been proposed in this analysis to study the hard turning of AISI 51200 hardened steel using a self-propelled rotary cutting tool. The model is developed by utilizing the explicit coupled temperature displacement analysis in the presence of realistic boundary conditions. This model does not take into account any assumptions regarding the heat partitioning and the tool-workpiece contact area. The model can predict the cutting forces, chip flow, induced stresses, and the generated temperature on the cutting tool and the workpiece. The nodal temperatures and heat flux data from the chip formation analysis are used to achieve steady-state temperatures on the cutting tool in the heat transfer analysis. The model outcomes are compared with reported experimental data and a good agreement has been found.
Effect of Internal Microstructure Distribution on Quasi-Static Compression Behavior and Energy Absorption of Hollow Truss Structures
Authors: Ren Huilan , Shen Haiting , Ning Jianguo
Published: November 2020

In this work, hollow truss structures with different internal microstructure distributions, i.e., basic hollow truss structure (specimen HT), hollow truss structure with internal microstructure at joints (specimen HTSJ), and hollow truss structure with internal microstructure on tube walls (specimen HTSW), were designed and manufactured using a selective laser melting technique. The effect of internal microstructure distribution on quasi-static compressive behavior and energy absorption was investigated by experimental tests and numerical simulations. The experimental results show that compressive strength and specific compressive strength of specimen HTSW increase by nearly 50% and 14% compared to specimen HT, and its energy absorption per volume and mass also increase by 52% and 15% at a strain of 0.5, respectively. However, the parameters of specimen HTSJ exhibit limited improvement or even a decrease in different degrees in comparison to specimen HT. The numerical simulation indicates that internal microstructures change the bearing capacity and structural weaknesses of the cells, resulting in the different mechanical properties and energy absorptions of the specimens. Based on the internal microstructure design in this study, adding microstructures into the internal weaknesses of the cells parallel to the loading direction is an effective way to improve the compressive properties, energy absorption and compressive stability of hollow truss structures.
Micro-Architectural Investigation of Teleost Fish Rib Inducing Pliant Mechanical Property
Authors: Jiao Yu Yang , Okada Masahiro , Hara Emilio Satoshi , Xie Shi Chao , Nagaoka Noriyuki , Nakano Takayoshi , Matsumoto Takuya
Published: November 2020

Despite the fact that various reports have been discussing bone tissue regeneration, precise bone tissue manipulation, such as controlling the physical properties of the regenerated bone tissue, still remains a big challenge. Here, we focused on the teleost fish ribs showing flexible and tough mechanical properties to obtain a deeper insight into the structural and functional features of bone tissue from different species, which would be valuable for the superior design of bone-mimicking materials. Herein, we examined their compositions, microstructure, histology, and mechanical properties. The first rib of Carassius langsdorfii showed a higher Young’s modulus with a small region of chondrocyte clusters compared with other smaller ribs. In addition, highly oriented collagen fibers and osteocytes were observed in the first rib, indicating that the longest first rib would be more mature. Moreover, the layer-by-layer structure of the oriented bone collagen was observed in each rib. These microarchitectural and compositional findings of fish rib bone would give one the useful idea to reproduce such a highly flexible rib bone-like material.
New Polyphenol-Containing LDL Nano-Preparations in Oxidative Stress and DNA Damage: A Potential Route for Cell-Targeted PP Delivery
Authors: Lewandowska Hanna , Kalinowska Monika
Published: November 2020

Low-density lipoprotein (LDL) preparations of the chosen polyphenols (PPs) were prepared for the first time in the literature. The solubility of the PPs in the lipidic core of the LDL increased with the increase of their lipophilicity. The anti-/pro-oxidative properties and toxicity of LDL-entrapped PPs toward A 2780 human ovarian cancer cells were examined. The obtained preparations were found to be stable in PBS, and characterized by low toxicity. A binding affinity study revealed that the uptake of PP-loaded LDL particles is non-receptor-specific under experimental conditions. The antioxidative potential of the obtained PPs-doped LDL preparations was shown to be higher than for the PPs themselves, probably due to facilitating transport of LDL preparations into the cellular milieu, where they can interact with the cellular systems and change the redox status of the cell. The PPs-loaded LDL displayed the highest protective effect against Fenton-type reaction induced oxidative DNA damage.
Ligand Control of 59Co Nuclear Spin Relaxation Thermometry
Authors: Ozvat Tyler M. , Johnson Spencer H. , Rappé Anthony K. , Zadrozny Joseph M.
Published: November 2020

Studying the correlation between temperature-driven molecular structure and nuclear spin dynamics is essential to understanding fundamental design principles for thermometric nuclear magnetic resonance spin-based probes. Herein, we study the impact of progressively encapsulating ligands on temperature-dependent 59Co T1 (spin–lattice) and T2 (spin–spin) relaxation times in a set of Co(III) complexes: K3[Co(CN)6] (1); [Co(NH3)6]Cl3 (2); [Co(en)3]Cl3 (3), en = ethylenediamine); [Co(tn)3]Cl3 (4), tn = trimethylenediamine); [Co(tame)2]Cl3 (5), tame = triaminomethylethane); and [Co(dinosar)]Cl3 (6), dinosar = dinitrosarcophagine). Measurements indicate that 59Co T1 and T2 increase with temperature for 1–6 between 10 and 60 °C, with the greatest ΔT1/ΔT and ΔT2/ΔT temperature sensitivities found for 4 and 3, 5.3(3)%T1/°C and 6(1)%T2/°C, respectively. Temperature-dependent T2* (dephasing time) analyses were also made, revealing the highest ΔT2*/ΔT sensitivities in structures of greatest encapsulation, as high as 4.64%T2*/°C for 6. Calculations of the temperature-dependent quadrupolar coupling parameter, Δe2qQ/ΔT, enable insight into the origins of the relative ΔT1/ΔT values. These results suggest tunable quadrupolar coupling interactions as novel design principles for enhancing temperature sensitivity in nuclear spin-based probes.
Update-Based Machine Learning Classification of Hierarchical Symbols in a Slowly Varying Two-Way Relay Channel
Authors: Kolář Jakub , Sýkora Jan , Hron Petr
Published: November 2020

This paper presents a stochastic inference problem suited to a classification approach in a time-varying observation model with continuous-valued unknown parameterization. The utilization of an artificial neural network (ANN)-based classifier is considered, and the concept of a training process via the backpropagation algorithm is used. The main objective is the minimization of resources required for the training of the classifier in the parametric observation model. To reach this, it is proposed that the weights of the ANN classifier vary continuously with the change of the observation model parameters. This behavior is then used in an update-based backpropagation algorithm. This proposed idea is demonstrated on several procedures, which re-use previously trained weights as prior information when updating the classifier after a channel phase change. This approach successfully saves resources needed for re-training the ANN. The new approach is verified via a simulation on an example communication system with the two-way relay slowly fading channel.
A Mathematical Model of Longitudinal Waves Incident at a Free Surface of a Pre-Stressed Dissipative Half-Space
Authors: Selim Mahmoud M. , Nofal Taher A.
Published: November 2020

The aim of this work is to study the behavior of reflection of a longitudinal wave at a free surface of dissipative half-space under the effects of compressive initial stresses. When a longitudinal wave is incident on the free surface of an elastic dissipative half-space, two damped waves (Primarywaves and secondary waves are reflected. Among of these waves, P-waves are affected by compressional initial stresses. The governing equation and corresponding closed-form solutions are derived based on Biot’s incremental deformation theory. The equations of motion are solved analytically and the influence of initial stress parameter on the reflection coefficient of P-wave incidents at the free surface of dissipative half-space is studied in detail. Numerical computations are performed for actual Earth crust and the results analyzing the incident of longitudinal waves are discussed and presented graphically. The analytical solutions and numerical results reveal that the compressive initial stress parameter has notable effects on the reflection coefficient of longitudinal wave incidents on the free surface of dissipative medium. In addition, it has been observed that the presence of compressive initial stresses increases the phase velocity of the longitudinal waves. To the authors’ best knowledge, effects of compressive initial stresses on the reflection coefficients of the incident longitudinal wave on a free surface of dissipative half-space have not been studied before. Since the actual Earth is subject to initial stresses due to different resources, understanding the influences of compressive initial stresses on the reflection coefficient of a longitudinal wave helps seismologists and earthquake engineers to get accurate results of the reflection coefficients of seismic waves propagation in the Earth. Thus, the present study would be useful for seismology and earthquake engineering fields and further study about the nature of seismic waves.
Influence of Laser Texturing on Microstructure, Surface and Corrosion Properties of Ti-6Al-4V
Authors: Conradi Marjetka , Kocijan Aleksandra , Klobčar Damjan , Godec Matjaž
Published: November 2020

We present the modification of Ti-6Al-4V surfaces with a diode end-pumped Nd:YVO4 laser by varying the distance between laser-produced micro(μ)-channels. We analyzed the influence of laser texturing on the morphology, microstructure, surface and corrosion properties of Ti-6Al-4V. SEM imaging reveals a characteristic μ-channel pattern with different scan line separations, while electron backscatter diffraction (EBSD) indicates that laser texturing with the current parameters influences the microstructure up to 2 µm deep with the most significant influence at the tips, where melting and rapid solidification occur. The Vickers hardness test indicates a surface hardening effect of the laser-textured compared to the as-received Ti-6Al-4V surfaces. The XPS analysis showed that the oxide layer on the laser-textured samples was considerably thicker compared to the as-received sample, at 20 and 7 nm, respectively. We observed that the wettability was strongly correlated with the scan line separation. The results show increased hydrophobicity with increased scan line separation. The corrosion resistance was improved for laser-textured surfaces compared to the as-received surface and increased with the scan line separation.
Production of Plant-Associated Volatiles by Select Model and Industrially Important Streptomyces spp.
Authors: Cheng Zhenlong , McCann Sean , Faraone Nicoletta , Clarke Jody-Ann , Hudson E. Abbie , Cloonan Kevin , Hillier N. Kirk , Tahlan Kapil
Published: November 2020

The Streptomyces produce a great diversity of specialized metabolites, including highly volatile compounds with potential biological activities. Volatile organic compounds (VOCs) produced by nine Streptomyces spp., some of which are of industrial importance, were collected and identified using gas chromatography–mass spectrometry (GC-MS). Biosynthetic gene clusters (BGCs) present in the genomes of the respective Streptomyces spp. were also predicted to match them with the VOCs detected. Overall, 33 specific VOCs were identified, of which the production of 16 has not been previously reported in the Streptomyces. Among chemical classes, the most abundant VOCs were terpenes, which is consistent with predicted biosynthetic capabilities. In addition, 27 of the identified VOCs were plant-associated, demonstrating that some Streptomyces spp. can also produce such molecules. It is possible that some of the VOCs detected in the current study have roles in the interaction of Streptomyces with plants and other higher organisms, which might provide opportunities for their application in agriculture or industry.
Evaluation of Recommended Water Sample Collection Methods and the Impact of Holding Time on Legionella Recovery and Variability from Healthcare Building Water Systems
Authors: Hirsh Marisa B. , Baron Julianne L. , Mietzner Sue M. , Rihs John D. , Yassin Mohamed H. , Stout Janet E.
Published: November 2020

Water safety and management programs (WSMP) utilize field measurements to evaluate control limits and monitor water quality parameters including Legionella presence. This monitoring is important to verify that the plan is being implemented properly. However, once it has been determined when and how to sample for Legionella, it is important to choose appropriate collection and processing methods. We sought to compare processing immediate and flushed samples, filtration of different volumes collected, and sample hold times. Hot water samples were collected immediately and after a 2-min flush. These samples were plated directly and after filtration of either 100 mL, 200 mL, or 1 L. Additionally, unflushed samples were collected and processed immediately and after 1, 24, and 48 h of hold time. We found that flushed samples had significant reductions in Legionella counts compared to immediate samples. Processing 100 mL of that immediate sample both directly and after filter concentration yielded the highest concentration and percent sample positivity, respectively. We also show that there was no difference in culture values from time 0 compared to hold times of 1 h and 24 h. At 48 h, there were slightly fewer Legionella recovered than at time 0. However, Legionella counts were so variable based on sampling location and date that this hold time effect was minimal. The interpretation of Legionella culture results depends on the sample collection and processing methods used, as these can have a huge impact on the success of sampling and the validation of control measures.
Interactions between Candida albicans and Enterococcus faecalis in an Organotypic Oral Epithelial Model
Authors: Krishnamoorthy Akshaya Lakshmi , Lemus Alex A. , Solomon Adline Princy , Valm Alex M. , Neelakantan Prasanna
Published: November 2020

Candida albicans as an opportunistic pathogen exploits the host immune system and causes a variety of life-threatening infections. The polymorphic nature of this fungus gives it tremendous advantage to breach mucosal barriers and cause oral and disseminated infections. Similar to C. albicans, Enterococcus faecalis is a major opportunistic pathogen, which is of critical concern in immunocompromised patients. There is increasing evidence that E. faecalis co-exists with C. albicans in the human body in disease samples. While the interactive profiles between these two organisms have been studied on abiotic substrates and mouse models, studies on their interactions on human oral mucosal surfaces are non-existent. Here, for the first time, we comprehensively characterized the interactive profiles between laboratory and clinical isolates of C. albicans (SC5314 and BF1) and E. faecalis (OG1RF and P52S) on an organotypic oral mucosal model. Our results demonstrated that the dual species biofilms resulted in profound surface erosion and significantly increased microbial invasion into mucosal compartments, compared to either species alone. Notably, several genes of C. albicans involved in tissue adhesion, hyphal formation, fungal invasion, and biofilm formation were significantly upregulated in the presence of E. faecalis. By contrast, E. faecalis genes involved in quorum sensing, biofilm formation, virulence, and mammalian cell invasion were downregulated. This study highlights the synergistic cross-kingdom interactions between E. faecalis and C. albicans in mucosal tissue invasion.
Cut-Lengths of Perennial Ryegrass Leaf-Blades Influences In Vitro Fermentation by the Anaerobic Fungus Neocallimastix frontalis
Authors: Jimenez Hugo R. , Edwards Joan E. , Sanderson Ruth , Kingston-Smith Alison H. , McEwan Neil R. , Theodorou Michael K.
Published: November 2020

Anaerobic fungi in the gut of domesticated and wild mammalian herbivores play a key role in the host’s ability to utilize plant biomass. Due to their highly effective ability to enzymatically degrade lignocellulose, anaerobic fungi are biotechnologically interesting. Numerous factors have been shown to affect the ability of anaerobic fungi to break down plant biomass. However, methods to reduce the non-productive lag time in batch cultures and the effect of leaf-blade cut-length and condition on the fungal fermentation are not known. Therefore, experimentation using a novel gas production approach with pre-grown, axenic cultures of Neocallimastix frontalis was performed using both fresh and air-dried perennial ryegrass leaf-blades of different cut-lengths. The methodology adopted removed the lag-phase and demonstrated the digestion of un-autoclaved leaf-blades. Fermentation of leaf-blades of 4.0 cm cut-length produced 18.4% more gas yet retained 11.2% more apparent DM relative to 0.5 cm cut-length leaf-blades. Drying did not affect fermentation by N. frontalis, although an interaction between drying and leaf-blade cut-length was noted. Removal of the lag phase and the use of un-autoclaved substrates are important when considering the biotechnological potential of anaerobic fungi. A hypothesis based upon sporulation at cut surfaces is proposed to describe the experimental results.
Multidrug-Resistant Proteus mirabilis Strain with Cointegrate Plasmid
Authors: Shelenkov Andrey , Petrova Lyudmila , Fomina Valeria , Zamyatin Mikhail , Mikhaylova Yulia , Akimkin Vasiliy
Published: November 2020

Proteus mirabilis is a component of the normal intestinal microflora of humans and animals, but can cause urinary tract infections and even sepsis in hospital settings. In recent years, the number of multidrug-resistant P. mirabilis isolates, including the ones producing extended-spectrum β-lactamases (ESBLs), is increasing worldwide. However, the number of investigations dedicated to this species, especially, whole-genome sequencing, is much lower in comparison to the members of the ESKAPE pathogens group. This study presents a detailed analysis of clinical multidrug-resistant ESBL-producing P. mirabilis isolate using short- and long-read whole-genome sequencing, which allowed us to reveal possible horizontal gene transfer between Klebsiella pneumoniae and P. mirabilis plasmids and to locate the CRISPR-Cas system in the genome together with its probable phage targets, as well as multiple virulence genes. We believe that the data presented will contribute to the understanding of antibiotic resistance acquisition and virulence mechanisms for this important pathogen.
Chemical and Phase Reactions on the Contact between Refractory Materials and Slags, a Case from the 19th Century Zn-Pb Smelter in Ruda Śląska, Poland
Authors: Kupczak Krzysztof , Warchulski Rafał , Dulski Mateusz , Środek Dorota
Published: November 2020

Slags from the historic metallurgy of Zn-Pb ores are known for unique chemical and phase compositions. The oxides, silicates, aluminosilicates, and amorphous phases present therein often contain in the structure elements that are rare in natural conditions, such as Zn, Pb, As. The study focuses on processes occurring on the contact of the melted batch and the refractory materials that build the furnace, which lead to the formation of these phases. To describe them, chemical (X-ray fluorescence (XRF), inductively coupled plasma mass spectrometry (ICP-MS)) and petrological ((X-ray diffraction (XRD), electron probe micro-analyses (EPMA), Raman spectroscopy) analyses were performed on refractory material, slag, and contact of both. Two main types of reactions have been distinguished: gas/fluid- refractories and liquid- refractories. The first of them enrich the refractories with elements that migrate with the gas (Pb, K, Na, As, Zn) and transport the components building it (Fe, Mg, Ca) inward. Reactions between melted batch and refractory materials through gravitational differentiation and the melting of refractories lead to the formation of an aluminosilicate liquid with a high content of heavy elements. Cooling of this melt causes crystallization of minerals characteristic for slag, but with a modified composition, such as Fe-rich pyroxenes, Pb-rich K-feldspar, or PbO-As2O3-SiO2 glass.
Label-Free Visualization and Tracking of Gold Nanoparticles in Vasculature Using Multiphoton Luminescence
Authors: Burkitt Sean , Mehraein Mana , Stanciauskas Ramunas K. , Campbell Jos , Fraser Scott , Zavaleta Cristina
Published: November 2020

Gold nanoparticles continue to generate interest for use in several biomedical applications. Recently, researchers have been focusing on exploiting their dual diagnostic/therapeutic theranostic capabilities. Before clinical translation can occur, regulatory agencies will require a greater understanding of their biodistribution and safety profiles post administration. Previously, the real-time identification and tracking of gold nanoparticles in free-flowing vasculature had not been possible without extrinsic labels such as fluorophores. Here, we present a label-free imaging approach to examine gold nanoparticle (AuNP) activity within the vasculature by utilizing multiphoton intravital microscopy. This method employs a commercially available multiphoton microscopy system to visualize the intrinsic luminescent signal produced by a multiphoton absorption-induced luminescence effect observed in single gold nanoparticles at frame rates necessary for capturing real-time blood flow. This is the first demonstration of visualizing unlabeled gold nanoparticles in an unperturbed vascular environment with frame rates fast enough to achieve particle tracking. Nanoparticle blood concentration curves were also evaluated by the tracking of gold nanoparticle flow in vasculature and verified against known pre-injection concentrations. Half-lives of these gold nanoparticle injections ranged between 67 and 140 s. This label-free imaging approach could provide important structural and functional information in real time to aid in the development and effective analysis of new metallic nanoparticles for various clinical applications in an unperturbed environment, while providing further insight into their complex uptake and clearance pathways.
Silicon-Based Ag Dendritic Nanoforests for Light-Assisted Bacterial Inhibition
Authors: Huang Hung Ji , Chang Han-Wei , Lin Yang-Wei , Chuang Shao-Yi , Lin Yung-Sheng , Shiao Ming-Hua
Published: November 2020

Silver dendritic nanoforests (Ag-DNFs) on silicon (Ag-DNFs/Si) were synthesized through the fluoride-assisted Galvanic replacement reaction (FAGRR) method. The synthesized Ag-DNFs/Si were characterized by scanning electron microscopy, energy-dispersive X-ray spectrometry, inductively coupled plasma mass spectrometry (ICP-MS), reflection absorbance spectrometry, surface-enhanced Raman scattering spectrometry, and X-ray diffractometry. The Ag+ concentration in ICP-MS measurements indicated 1.033 mg/cm2 of deposited Ag synthesized for 200 min on Si substrate. The optical absorbance spectra indicated the induced surface plasmon resonance of Ag DNFs increased with the thickness of the Ag DNFs layer. Surface-enhanced Raman scattering measurement and a light-to-heat energy conversion test presented the superior plasmonic response of Ag-DNFs/Si for advanced applications. The Ag-DNFs/Si substrate exhibited high antibacterial activity against Escherichia coli and Staphylococcus aureus. The large surface area of the dense crystal Ag DNFs layer resulted in high antibacterial efficiency. The plasmonic response in the metal–crystal Ag DNFs under external light illumination can supply energy to enhance bacterial inhibition. High-efficiency plasmonic heating by the dense Ag DNFs can lead to localized bacterial inhibition. Thus, the Ag-DNFs/Si substrate has excellent potential for antibacterial applications.
Seeing Is Believing: Visualizing Circular RNAs
Authors: Bejugam Pruthvi Raj , Das Aniruddha , Panda Amaresh Chandra
Published: November 2020

Advancement in the RNA sequencing techniques has discovered hundreds of thousands of circular RNAs (circRNAs) in humans. However, the physiological function of most of the identified circRNAs remains unexplored. Recent studies have established that spliceosomal machinery and RNA-binding proteins modulate circRNA biogenesis. Furthermore, circRNAs have been implicated in regulating crucial cellular processes by interacting with various proteins and microRNAs. However, there are several challenges in understanding the mechanism of circRNA biogenesis, transport, and their interaction with cellular factors to regulate cellular events because of their low abundance and sequence similarity with linear RNA. Addressing these challenges requires systematic studies that directly visualize the circRNAs in cells at single-molecule resolution along with the molecular regulators. In this review, we present the design, benefits, and weaknesses of RNA imaging techniques such as single-molecule RNA fluorescence in situ hybridization and BaseScope in fixed cells and fluorescent RNA aptamers in live-cell imaging of circRNAs. Furthermore, we propose the potential use of molecular beacons, multiply labeled tetravalent RNA imaging probes, and Cas-derived systems to visualize circRNAs.
Update and New Epidemiological Aspects about Grapevine Yellows in Chile
Authors: Quiroga Nicolás , Gamboa Camila , Soto Daniela , Pino Ana Maria , Zamorano Alan , Campodonico Juan , Alma Alberto , Bertaccini Assunta , Fiore Nicola
Published: November 2020

To date, phytoplasmas belonging to six ribosomal subgroups have been detected to infect grapevines in Chile in 36 percent of the sampled plants. A new survey on the presence of grapevine yellows was carried out from 2016 to 2020, and 330 grapevine plants from the most important wine regions of the country were sampled and analyzed by nested PCR/RFLP analyses. Phytoplasmas enclosed in subgroups 16SrIII-J and 16SrVII-A were identified with infection rates of 17% and 2%, respectively. The vineyards in which the phytoplasma-infected plants were detected were further inspected to identify alternative host plants and insects of potential epidemiological relevance. Five previously unreported plant species resulted positive for 16SrIII-J phytoplasma (Rosa spp., Brassica rapa, Erodium spp., Malva spp. and Rubus ulmifolius) and five insect species were fully or partially identified (Amplicephalus ornatus, A. pallidus, A. curtulus, Bergallia sp., Exitianus obscurinervis) as potential vectors of 16SrIII-J phytoplasmas. The 16SrVII-A phytoplasmas were not detected in non-grape plant species nor in insects. This work establishes updated guidelines for the study, management, and prevention of grapevine yellows in Chile, and in other grapevine growing regions of South America.
Humoral and Cell-Mediated Immune Response Validation in Calves after a Live Attenuated Vaccine of Babesia bigemina
Authors: Rauf Umber , Suleman Muhammad , Abid Asadullah , Jamil Hamna , Menghwar Harish , Durrani Aneela Zameer , Rashid Muhammad Imran , Akbar Haroon
Published: November 2020

The current vaccines to control bovine Babesia bigemina (B. bigemina) infection are not fully protective and vaccination failures incur heavy losses to the cattle industry around the world. Using modified micro-aerophilous stationary phase, we developed a culture-derived attenuated live vaccine against B. bigemina and tested a single subcutaneous inoculation of 2 × 108 infected erythrocytes in calves. The protection was measured after a lethal intravenous challenge with 5 × 108 virulent calf-derived B. bigemina. Our results demonstrated that a single shot of attenuated vaccine was capable of inducing robust humoral and cell-mediated immune responses in calves. We found a significant increase in the IgG antibody titers post-challenge and a strong proliferation of both CD4+ and CD8+ T cells contributing towards the protection. Our vaccine provided complete protection and parasitic clearance, which was followed for more than 100 days post-challenge. This immunity against babesiosis was directly linked to strong humoral responses; however, the parasitic clearance was attributed to significant T cells effector responses in vaccinated calves as compared to the infected control calves. We anticipate that these results will be helpful in the development of more efficient culture-derived vaccines against Babesia infections, thus reducing significant global economic losses to farmers and the cattle industry.
Identification of Clinically Relevant Streptococcus and Enterococcus Species Based on Biochemical Methods and 16S rRNA, sodA, tuf, rpoB, and recA Gene Sequencing
Authors: Kosecka-Strojek Maja , Wolska Mariola , Żabicka Dorota , Sadowy Ewa , Międzobrodzki Jacek
Published: November 2020

Streptococci and enterococci are significant opportunistic pathogens in epidemiology and infectious medicine. High genetic and taxonomic similarities and several reclassifications within genera are the most challenging in species identification. The aim of this study was to identify Streptococcus and Enterococcus species using genetic and phenotypic methods and to determine the most discriminatory identification method. Thirty strains recovered from clinical samples representing 15 streptococcal species, five enterococcal species, and four nonstreptococcal species were subjected to bacterial identification by the Vitek® 2 system and Sanger-based sequencing methods targeting the 16S rRNA, sodA, tuf, rpoB, and recA genes. Phenotypic methods allowed the identification of 10 streptococcal strains, five enterococcal strains, and four nonstreptococcal strains (Leuconostoc, Granulicatella, and Globicatella genera). The combination of sequencing methods allowed the identification of 21 streptococcal strains, five enterococcal strains, and four nonstreptococcal strains. The 16S rRNA and rpoB genes had the highest identification potential. Only a combination of several molecular methods was sufficient for unambiguous confirmation of species identity. This study will be useful for comparison of several identification methods, both those used as a first choice in routine microbiology and those used for final confirmation.
Resistance and Virulence Features of Bacteroides spp. Isolated from Abdominal Infections in Romanian Patients
Authors: Pricop Gabriela Roxana , Gheorghe Irina , Pircalabioru Gratiela Gradisteanu , Cristea Violeta , Popa Marcela , Marutescu Luminita , Chifiriuc Mariana Carmen , Mihaescu Grigore , Bezirtzoglou Eugenia
Published: November 2020

Anaerobic bacteria are predominant residents of the normal microbiota of the skin and mucous membranes but are also known to be associated with a number of human infections including peritonitis, appendicitis, abscesses, ulcers and wound infections. Herein, we investigate the antibiotic resistance profiles as well as the genetic support of antibiotic resistance and virulence determinants of anaerobic bacteria isolated from intra-abdominal infections. The study was performed on 198 Romanian patients from which different clinical samples were taken intra-operatory and sent for microbiological analyses. From the total number of isolated strains, a subset of 75 Bacteroides spp. were selected and further investigated for antibiotic resistance and virulence features, at phenotypic and genetic level. Our results obtained through the analysis of a significant number of Bacteroides strains could shed light on the virulence potential and mechanisms by which anaerobic bacteria can cause endogenous infections.
Molecular Typing of Neisseria gonorrhoeae Clinical Isolates in Russia, 2018–2019: A Link Between penA Alleles and NG-MAST Types
Authors: Kandinov Ilya , Dementieva Ekaterina , Kravtsov Dmitry , Chestkov Alexander , Kubanov Alexey , Solomka Victoria , Deryabin Dmitry , Gryadunov Dmitry , Shaskolskiy Boris
Published: November 2020

This work aimed to study penA gene polymorphisms in clinical isolates of Neisseria gonorrhoeae collected in Russia in 2018–2019 and the contribution of the penA allele type to susceptibility to β-lactam antibiotics. A total of 182 isolates were analyzed. penA allele types were determined by sequencing, and the minimum inhibitory concentrations (MICs) of benzylpenicillin and ceftriaxone were measured. The influence of genetic factors on MICs was evaluated by regression analysis. All isolates were susceptible to ceftriaxone, and 40.1% of isolates were susceptible to penicillin. Eleven penA allele types were identified. The mosaic type XXXIV penA allele and the Gly120Lys substitution in PorB made the greatest contributions to increasing the ceftriaxone MIC; the presence of the blaTEM plasmid, Gly120Asp, Ala121Gly/Asn substitutions in PorB, and the adenine deletion in the promoter region of the mtrR gene caused an increase in the penicillin MIC. Among 61 NG-MAST types identified, the most frequent were types 228, 807, 9486, 1993, and 6226. A link between penA alleles and Neisseria gonorrhoeae multi-antigen sequence typing (NG-MAST) types was established. Resistance to two groups of β-lactam antibiotics was associated with non-identical changes in penA alleles. To prevent the emergence of ceftriaxone resistance in Russia, NG-MAST genotyping must be supplemented with penA allele analysis.
Ellipticity of High-Order Harmonics Generated by Aligned Homonuclear Diatomic Molecules Exposed to an Orthogonal Two-Color Laser Field
Authors: Habibović Dino , Milošević Dejan B.
Published: November 2020

We investigate emission rate and ellipticity of high-order harmonics generated exposing a homonuclear diatomic molecule, aligned in the laser-field polarization plane, to a strong orthogonally polarized two-color (OTC) laser field. The linearly polarized OTC-field components have frequencies rω and sω, where r and s are integers. Using the molecular strong-field approximation with dressed initial state and undressed final state, we calculate the harmonic emission rate and harmonic ellipticity for frequency ratios 1:2 and 1:3. The obtained quantities depend strongly on the relative phase between the laser-field components. We show that with the OTC field it is possible to generate elliptically polarized high-energy harmonics with high emission rate. To estimate the relative phase for which the emission rate is maximal we use the simple man’s model. In the harmonic spectra as a function of the molecular orientation there are two types of minima, one connected with the symmetry of the molecular orbital and the other one due to destructive interference between different contributions to the recombination matrix element, where we take into account that the electron can be ionized and recombine at the same or different atomic centers. We derive a condition for the interference minima. These minima are blurred in the OTC field except in the cases where the highest occupied molecular orbital is modeled using only s or only p orbitals in the linear combination of the atomic orbitals. This allows us to use the interference minima to assess which atomic orbitals are dominant in a particular molecular orbital. Finally, we show that the harmonic ellipticity, presented in false colors in the molecular-orientation angle vs. harmonic-order plane, can be large in particular regions of this plane. These regions are bounded by the curves determined by the condition that the harmonic ellipticity is approximately zero, which is determined by the minima of the T-matrix contributions parallel and perpendicular to the fundamental component of the OTC field.
Switching from Electron to Hole Transport in Solution-Processed Organic Blend Field-Effect Transistors
Authors: Fidyk Julia , Waliszewski Witold , Sleczkowski Piotr , Kiersnowski Adam , Pisula Wojciech , Marszalek Tomasz
Published: November 2020

Organic electronics became an attractive alternative for practical applications in complementary logic circuits due to the unique features of organic semiconductors such as solution processability and ease of large-area manufacturing. Bulk heterojunctions (BHJ), consisting of a blend of two organic semiconductors of different electronic affinities, allow fabrication of a broad range of devices such as light-emitting transistors, light-emitting diodes, photovoltaics, photodetectors, ambipolar transistors and sensors. In this work, the charge carrier transport of BHJ films in field-effect transistors is switched from electron to hole domination upon processing and post-treatment. Low molecular weight n-type N,N′-bis(n-octyl)-(1,7&1,6)-dicyanoperylene-3,4:9,10-bis(dicarboximide) (PDI8-CN2) was blended with p-type poly[2,5-bis(3-tetradecylthiophene-2-yl)thieno[3,2-b]thiophene] (PBTTT-C14) and deposited by spin-coating to form BHJ films. Systematic investigation of the role of rotation speed, solution temperature, and thermal annealing on thin film morphology was performed using atomic force microscopy, scanning electron microscopy, and grazing incidence wide-angle X-ray scattering. It has been determined that upon thermal annealing the BHJ morphology is modified from small interconnected PDI8-CN2 crystals uniformly distributed in the polymer fraction to large planar PDI8-CN2 crystal domains on top of the blend film, leading to the switch from electron to hole transport in field-effect transistors.
Experimental and Comparative RANS/URANS Investigations on the Effect of Radius of Volute Tongue on the Aerodynamics and Aeroacoustics of a Sirocco Fan
Authors: Rui Xiaocheng , Lin Limin , Wang Junkui , Ye Xinxue , He Haijiang , Zhang Wei , Zhu Zuchao
Published: November 2020

The geometry of volute tongue is crucial in the design of Sirocco fans. The size of the volute tongue determines its relative position and distance from the impeller which affects the local flow characteristics and thus the aerodynamic and aeroacoustic performances of the fan. In this work, we performed experimental and numerical investigations on the effect of volute tongue radius on the aerodynamic and aeroacoustic characteristics of a Sirocco fan. The internal flow characteristics are analyzed and discussed in terms of the spatial distribution and temporal variation of pressure and streamlines, the pulsating behaviors of pressure both in the impeller and on the volute surface with emphasis in the volute tongue region, the variation of passage flow with the rotation of impeller and the aeroacoustic features of the fan. We conducted numerical simulations using both steady Reynolds-Averaged Navier-Stokes (RANS) and unsteady Reynolds-Averaged Navier-Stokes (URANS) approaches with realizable k-ε turbulence model with rotation effect correction and the results are compared against the experimental data to assess the prediction capability and accuracy in qualitative and quantitative manners. Experimental and numerical results show that as the volute tongue radius increases, the static pressure rises as well as the far-field noise of the fan and pronounced fluctuation of flow is observed within the whole impeller and volute; the reversed flow in the passage of the impeller is reduced and the high-pressure region is found to be moving towards the outlet of the volute. The decreasing radius also enlarges the size of the adverse pressure gradient (APG) region on the volute tongue which contributes to the formation of recirculating flow. The comparative RANS and URANS simulations reveal that both approaches produce generally consistent results regarding the time-averaged flow although the URANS data are much closer to those of the experimental ones. However, the fluctuating flow which is not capable to be modeled by RANS still dominates for the present configuration and thus URANS is necessary for the accurate prediction of the flow details.