bluuebunny/crossref_metadata_2025 huggingface dataset:
Short-Term Low-Temperature Storage and Cryopreservation of Qihe Crucian Carp (Carassius auratus) Sperm
Authors: Shi Xi , Xu Jiayin , Hou Yujie , Wei Zhen , Guo Lufang , Ma Xiao , Wu Limin , Ma Wenge , Tian Xue , Waiho Khor , Li Xuejun
Published: February 2025

Qihe crucian carp Carassius auratus is one of the major economic aquacultural fish species in China. Due to environmental degradation and the impact of the reproduction mode of gynogenesis, the wild population of Qihe crucian carp exhibits an extremely low proportion of males. Therefore, it is imperative to develop the sperm preservation technology for Qihe crucian carp. In this study, the indicators, including sperm motility, sperm movement time, and sperm lifetime, were applied to evaluate the preservation effects of various extenders, cryoprotectant types and concentrations, and preservation manners, thus establishing the techniques of sperm short-term storage and cryopreservation for Qihe crucian carp. The results showed that HBSS was the most suitable extender solution, in which the sperm motility reached 93.00%. Under room temperature (26 °C), the sperm motility showed no significant differences across six different cryopreservation solutions. Under low temperature (4 °C), a 15% MeOH solution presented the most effective preservation effects of sperm in the short-term low-temperature storage. For cryopreservation in liquid nitrogen, the sperm activity in 20% DMSO was significantly higher than that in other solutions, representing the optimal cryopreservation solution. Furthermore, it was found that the sperm activity preserved by liquid nitrogen was significantly higher than that preserved by the ultra-freezer (−80 °C), indicating that the ultra-freezer cannot substitute for liquid nitrogen in cryopreservation. This is the first study to investigate the preservation of Qihe crucian carp sperm, providing valuable technical support for both genetic resource conservation and artificial breeding programs.
Multiplex PCR Pneumonia Panel in Critically Ill Patients Did Not Modify Mortality: A Cohort Study
Authors: Riaño-Sánchez Luisa Fernanda , Alvarez-Moreno Carlos Arturo , Godoy Marcela , Sierra Claudia Rocío , Castañeda Margarita Inés , Cortés Jorge Alberto
Published: February 2025

In critically ill patients, identification of the pathogen may allow for the timely adjustment of antibiotics and improved outcomes. Background/Objectives: The aim of the study was to assess whether performing a multiplex PCR pneumonia panel (PN-panel) in patients with pneumonia in the intensive care unit (ICU) had any effect on mortality or other important clinical outcomes. Methods: A retrospective cohort study was conducted on adult patients with pneumonia who required ICU admission in four institutions in Bogotá between November 2019 and June 2023. Mortality at 30 days, the length of the hospital and ICU stay, the duration of antibiotics, and their association with the PN-panel performance were evaluated using an inverse probability of the treatment weighting to adjust for covariates and potential confounders. Results: A total of 304 patients were included, including 150 with PN-panel, with a mean age of 65.0 years (SD 14.6). SARS-CoV-2 was the primary etiologic agent in 186 (61.2%) patients, and 256 (84.2%) patients had community-acquired pneumonia. No association was found between 30-day mortality and the PN-panel, with a HR of 1.14 (CI 95% 0.76–1.70), although the assessment by an infectious disease specialist was associated with a lower mortality HR of 0.29 (CI 95% 0.19–0.45). There was no association between the PN-panel and antimicrobial therapy duration or other clinical outcomes. Conclusions: The use of the PN-panel was not associated with changes in mortality, the duration of antibiotics, or hospital and ICU stays. To acquire greater rational decision-making, microbiological data produced by this test should be interpreted with aid of an antimicrobial stewardship program oriented by an infectious disease team that could take the clinical data and integrate the information provided.
Sulforaphane Restores Mitochondrial β-Oxidation and Reduces Renal Lipid Accumulation in a Model of Releasing Unilateral Ureteral Obstruction
Authors: Aranda-Rivera Ana Karina , Amador-Martínez Isabel , Aparicio-Trejo Omar Emiliano , León-Contreras Juan Carlos , Hernández-Pando Rogelio , Saavedra Emma , García-Arroyo Fernando E. , Pedraza-Chaverri José , Sánchez-Lozada Laura Gabriela , Tapia Edilia
Published: February 2025

Obstructive nephropathy (ON), characterized by urine flow disruption, can induce chronic kidney disease (CKD). Although the release of the obstruction is performed as the primary intervention, renal pathology often persists and progresses. Accordingly, the murine model of releasing unilateral ureteral obstruction (RUUO) is valuable for investigating the molecular events underlying renal damage after obstruction release. Remarkably, after RUUO, disturbances such as oxidative stress, inflammation, lipid accumulation, and fibrosis continue to increase. Mitochondrial dysfunction contributes to fibrosis in the UUO model, but its role in RUUO remains unclear. Additionally, the impact of using antioxidants to restore mitochondrial function and prevent renal fibrosis in RUUO has not been determined. This study aimed to determine the therapeutic effect of pre-administering the antioxidant sulforaphane (SFN) in the RUUO model. SFN was administered 1 day before RUUO to evaluate mitochondrial biogenesis, fatty acids (FA) metabolism, bioenergetics, dynamics, and mitophagy/autophagy mechanisms in the kidney. Our data demonstrated that SFN enhanced mitochondrial biogenesis and reestablished mitochondrial oxygen consumption and β-oxidation. These effects collectively reduced lipid accumulation and normalized mitochondrial dynamics, mitophagy, and autophagy, thereby mitigating fibrosis after obstruction. Our findings suggest that SFN holds promise as a potential therapeutic agent in ON-induced CKD progression in RUUO and opens new avenues in studying antioxidant molecules to treat this disease.
Morinda citrifolia L.: A Comprehensive Review on Phytochemistry, Pharmacological Effects, and Antioxidant Potential
Authors: Hou Silu , Ma Danyang , Wu Shaofeng , Hui Qiaoyue , Hao Zhihui
Published: February 2025

Morinda citrifolia L. (M. citrifolia), commonly referred to as noni, a Polynesian medicinal plant with over 2000 years of traditional use, has garnered global interest for its rich repertoire of antioxidant phytochemicals, including flavonoids (kaempferol, rutin), iridoids (aucubin, asperulosidic acid, deacetylasperulosidic acid, asperuloside), polysaccharides (nonioside A), and coumarins (scopoletin). This comprehensive review synthesizes recent advances (2018–2023) on noni’s bioactive constituents, pharmacological properties, and molecular mechanisms, with a focus on its antioxidant potential. Systematic analyses reveal that noni-derived compounds exhibit potent free radical scavenging capacity (e.g., 2,2-Diphenyl-1-picrylhydrazyl/2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonicacid) (DPPH/ABTS) inhibition), upregulate endogenous antioxidant enzymes (Superoxide Dismutase (SOD), Catalase (CAT), Glutathione Peroxidase (GPx)), and modulate key pathways such as Nuclear factor erythroid 2-related factor 2/Kelch-like ECH-associated protein 1 (Nrf2/Keap1) and Nuclear Factor kappa-B (NF-κB). Notably, polysaccharides and iridoids demonstrate dual antioxidant and anti-inflammatory effects via gut microbiota regulation. This highlights the plant’s potential for innovation in the medical and pharmaceutical fields. However, it is also recognized that further research is needed to clarify its mechanisms of action and ensure its safety for widespread application. We emphasize the need for mechanistic studies to bridge traditional knowledge with modern applications, particularly in developing antioxidant-rich nutraceuticals and sustainable livestock feed additives. This review underscores noni’s role as a multi-target antioxidant agent and provides a roadmap for future research to optimize its health benefits.
Antioxidant and Antidiabetic Potential of the Antarctic Lichen Gondwania regalis Ethanolic Extract: Metabolomic Profile and In Vitro and In Silico Evaluation
Authors: Torres-Benítez Alfredo , Ortega-Valencia José Erick , Jara-Pinuer Nicolás , Ley-Martínez Jaqueline Stephanie , Velarde Salvador Herrera , Pereira Iris , Sánchez Marta , Gómez-Serranillos María Pilar , Sasso Ferdinando Carlo , Simirgiotis Mario , Caturano Alfredo
Published: February 2025

Lichens are an important source of diverse and unique secondary metabolites with recognized biological activities through experimental and computational procedures. The objective of this study is to investigate the metabolomic profile of the ethanolic extract of the Antarctic lichen Gondwania regalis and evaluate its antioxidant and antidiabetic activities with in vitro, in silico, and molecular dynamics simulations. Twenty-one compounds were tentatively identified for the first time using UHPLC/ESI/QToF/MS in negative mode. For antioxidant activity, the DPPH assay showed an IC50 value of 2246.149 µg/mL; the total phenolic content was 31.9 mg GAE/g, the ORAC assay was 13.463 µmol Trolox/g, and the FRAP assay revealed 6.802 µmol Trolox/g. Regarding antidiabetic activity, enzyme inhibition yielded IC50 values of 326.4513 µg/mL for pancreatic lipase, 19.49 µg/mL for α-glucosidase, and 585.216 µg/mL for α-amylase. Molecular docking identified sekikaic acid as the most promising compound, with strong binding affinities to catalytic sites, while molecular dynamics confirmed its stability and interactions. Toxicological and pharmacokinetic analyses supported its drug-like potential without significant risks. These findings suggest that the ethanolic extract of Gondwania regalis is a promising source of bioactive compounds for developing natural antioxidant and antidiabetic therapies.
Improvement of Terrain Entropy Calculation for Grid Digital Elevation Models Considering Spatial Structural Features
Authors: Zhou Fangbin , Yao Tianyi , Bian Junwei , Xiao Yun
Published: February 2025

Existing methods for calculating terrain entropy in grid digital elevation models (DEMs) often face computational anomalies in specific topographies within small windows. To address this issue, an improved method was developed based on the Euclidean distance approach. This method was inspired by Claramunt’s technique of weighting information entropy by the average distance between points with the same value and different values. Specifically, vectors were formed between grid points and categorized by value consistency and relative positions. Those formed between points of different values were classified by the value of the starting point as well as parallel and adjacent relationships. This comprehensive grouping strategy was integrated into distance calculations, becoming a new probability operator that accurately reflects terrain spatial characteristics. Experimental verification confirms that the method proposed aligns with the fundamental concept of entropy, yielding a regression equation of y=0.011ln⁡x+0.463 with a coefficient of determination of 94.73%, a reliability of 44.015, and a measurement ability of 0.757. For the mixed iterative images with gradually increasing spatial disorder, their entropy values should follow a logarithmic trend. Therefore, a logarithmic function is used for fitting. A determination coefficient greater than 50% indicates that the method adheres to the original definition of entropy and is effective in capturing the increasing spatial disorder of the grid DEM. A lower reliability value suggests smoother data computation between the two iterations. A lower measurement ability value indicates slower convergence for grid DEMs with gradually increasing spatial disorder. The improved method was also tested on simulated and real DEMs, and the results showed a strong correlation between calculated terrain entropy values and terrain complexity. By effectively capturing spatial information changes, this approach overcomes the shortcoming of computational anomalies and demonstrates high reliability in terrain entropy calculation in grid DEMs.
Comparative Analysis of Deep Learning Methods for Real-Time Estimation of Earthquake Magnitude
Authors: Shen Xuanye , Hou Baorui , Lu Jianqi , Li Shanyou
Published: February 2025

In recent years, although a variety of deep learning models have been developed for magnitude estimation, the complex and variable nature of earthquakes limits the generalizability and accuracy of these models. In this study, we selected the waveform data of the Japan earthquake. We applied four deep learning techniques (MagNet combined with bidirectional long- and short-term memory network Bi-LSTM, DCRNN with deepened CNN layers, DCRNNAmp with the introduction of a global scale factor, and Exams with a multilayered CNN architecture) for real-time magnitude estimation. By comparing the estimation errors of each model in the first 3 s after the earthquake, it is found that the DCRNNAmp performs the best, with an MAE of 0.287, an RMSE of 0.397, and an R2 of 0.737 in the first 3 s after the arrival of the P-wave, and the inclusion of S-wave seismic-phase information is found to significantly improve the accuracy of the magnitude estimation, which suggests that S-wave seismic-phase waveform features can enrich the model’s understanding of the relationship between the seismic phases. It shows that S-wave phase waveform features can enrich the model’s knowledge of the relationship between seismic fluctuations and magnitude. The epicentral distance positively correlates with the magnitude estimation, and the model can converge faster with the improved signal-to-noise ratio. Despite the shortcomings of model design and opaque internal mechanisms, this study provides important evidence for deep learning in earthquake estimation, demonstrating its potential to improve the accuracy of on-site earthquake early warning (EEW) systems. The estimation capability can be further improved by optimizing the model and exploring new features.
Relation of Dominant Leg Use with Functional Symmetries in Young Football Players of Different Age Groups
Authors: Železnik Matjaž , Čuk Ivan , Šibanc Karmen
Published: February 2025

The aim of the study was to determine whether football players of different age groups who use their dominant leg more frequently when playing football exhibit greater functional asymmetries between the right and left leg. The study included 116 football players (age groups from U12 to U19). The number of touches of the ball with the right and left leg was determined by detailed video analysis. The data were used to calculate the percentage of use of the dominant leg. Functional asymmetries were determined based on measurements of motor skills in the field and measurements with a portable dual-force plate. Significant correlations were found between the percentage of the dominant leg use and the differences between legs in kicking the ball (ball flight speed) from a standing position in the entire sample, from a running position in the entire sample and in the U17 age group and in relative maximum power in the CMJ in the U15 age group. No significant correlations were found with differences between the legs when dribbling the ball with a change in direction. The proportion of use of the dominant leg correlates with some of the observed functional asymmetries in young football players of different age groups and varies across age groups.
Heat Transfer in Granular Material: Experimental Measurements and Parameters Identification of Macroscopic Heat Conduction Model
Authors: Ciesielski Mariusz , Grodzki Grzegorz
Published: February 2025

The paper presents experimental results regarding heat transfer in granular materials in the cylindrical domain during heating by the outer surface of the container. Sensors (K-type thermocouples) were used to measure the temperature changes at several points inside granular material (the fine-grained table salt was used in the experiment). Knowledge of measurement data allows the verification of a mathematical model (based on Fourier’s law) to describe the macroscopic heat conduction in granular materials. An iterative algorithm for the inverse heat conduction problem consisting of the estimation of the thermal diffusivity coefficient of granular material, the parameters of initial boundary conditions and the position of the thermocouple tips during the experiment was developed. Several computational simulations were performed. Based on the experimental results and the computational simulation results, one can conclude that the analytical solution of the direct heat conduction problem calculated for the optimal values obtained from the inverse heat conduction problem gave us the confirmation of the validity of Fourier’s model.
Design and Mechanical Behavior Research of Highway Guardrail Patrol Robot
Authors: Chang Hong , Zhao Guoqing , Tang Shufeng
Published: February 2025

Conducting risk assessments on highways is a critical task. This paper introduces a mobile platform designed for guardrail inspection robots to address the gap between the inspection requirements in road traffic management and the current capabilities of existing highway inspection robots. The platform is utilized for random vehicle inspections, road environment assessments, and transportation equipment evaluations. The robot is designed to operate on double-waveform beam guardrails and features an innovative adaptive dual-wheel tensioning mechanism, significantly enhancing its ability to adapt to the guardrail’s shape and joints. A mechanical model of the robot was developed, and the impact of the tension on the robot’s obstacle-crossing performance was analyzed and optimized through theoretical and simulation-based studies. Finally, a prototype of the robot was constructed, and a testing platform for the highway guardrails was established to evaluate the robot’s operational capabilities. The results demonstrate that the robot exhibits excellent performance in both operation and obstacle-crossing tasks.
Preparation, Characterization and Evaluation of the Antibacterial Activity of Ag Nanoparticles Embedded in Transparent Oxide Matrices
Authors: Gălbau Cristina-Ștefania , Idomir Mihaela , Vițelaru Cătălin , Kiss Adrian Emil , Parau Anca Constantina , Constantin Lidia Ruxandra , Dinu Mihaela , Pana Iulian , Vlădescu (Dragomir) Alina , Gaman Elena Laura , Moga Marius Alexandru , Mișarcă Cătălin , Vârciu Mihai , Irimie Claudia Alexandrina , Badea Mihaela
Published: February 2025

Daily exposure to contaminated environments and surfaces leads to serious health issues, increasing healthcare costs. Active materials that act against pathogens can effectively prevent their proliferation and contribute to increased protection against infections. In this contribution, nanostructured thin films containing silver are investigated, using SiO2 and TiO2 as transparent matrices to embed the Ag atoms. The thin transparent films were obtained via magnetron sputtering, using HiPIMS for Ag deposition and RF sputtering for oxides, in either an Ar or Ar/O2 environment. Atomic Force Microscopy provided information on coating topography and the thin films’ preferential growth on the textured polymer foil, X-Ray Diffraction highlighted the structural difference between different versions, Ultraviolet–Visible–Near-Infrared spectroscopy proved the thin films’ optical quality and their transparency and Energy-Dispersive X-ray Spectroscopy revealed the composition changes for different processes. The effect of O2 addition is analyzed and compared in terms of changes induced on the properties of the thin films. Following 24 h of incubation in a media containing 104 CFU/mL Escherichia coli, the TiO2+Ag sample with O2 addition showed the highest antibacterial effectiveness, as indicated by the largest inhibition zone. Experiments on selective media showed a hierarchy of efficiency, namely, TiO2+Ag+O2 > TiO2+Ag > SiO2+Ag.
Zirconium-Containing Metal–Organic Frameworks (MOFs) as Catalysts for Biomass Conversion
Authors: Rapeyko Anastasia , Llabrés i Xamena Francesc X.
Published: February 2025

In this work, we review some illustrative examples to evidence the potential of two archetypal Zr-containing MOFs, UiO-66 and MOF-808, as heterogeneous catalysts for converting biomass-derived products into valuable chemicals. The reactions are organized in three blocks, depending on the biomass source: carbohydrates, lipids, and other sources. Through this review, we will show that the chemical properties of these two Zr-MOFs are significantly different in terms of the nature and strength of acid sites, which largely depends on the number of missing linker defects of the solid and its hydration state. While hydrated UiO-66 bears relatively strong Brønsted-induced acid sites, dehydrated MOF-808 is more than competent as a Lewis acid catalyst. Therefore, we will pick one or the other catalyst depending on the particular demands of the catalytic transformation that we want to carry out.
Interface Design for Responsible Remote Driving: A Study on Technological Mediation
Authors: Variati Gabriella Emma , Fossa Fabio , Prakash Jai , Cheli Federico , Caruso Giandomenico
Published: February 2025

Remote driving, i.e., the capacity of controlling road vehicles at a distance, is an innovative transportation technology often associated with potential ethical benefits, especially when deployed to tackle urban traffic issues. However, prospected benefits could only be reaped if remote driving can be executed in a safe and responsible way. This paper builds on notions elaborated in the philosophical literature on technological mediation to offer a systematic examination of the extent to which current and emerging Human–Machine Interfaces contribute to hindering or supporting the exercise of responsibility behind the remote wheel. More specifically, the analysis discusses how video, audio, and haptic interfaces co-shape the remote driving experience and, at the same time, the operators’ capacity to drive responsibly. The multidisciplinary approach explored in this research offers a novel methodological framework to structure future empirical inquiries while identifying finely tuned multi-sensory HMIs and dedicated training as critical presuppositions to the remote drivers’ exercise of responsibility.
A Status Evaluation of Rock Instability in Metal Mines Based on the SPA–IAHP–PCN Model
Authors: Yan Fang , Li Xuan , Dong Longjun , Du Shengnan , Wang Hongwei , Sun Daoyuan
Published: February 2025

As one of the serious hazards in deep mining, rock instability will cause roof falls, rib spalling, rockburst, and other serious disasters. It will lead to significant casualties and property losses. Conducting risk assessments for rock instability is of significant importance. Firstly, an evaluation model called IAHP-SPA was proposed to address uncertainties in the weight determination process. Secondly, the Partial Connection Number (PCN) including the first-order PCN, the second-order PCN, the third-order PCN and the fourth-order PCN were introduced. Thus, a dynamic and comprehensive evaluation of rock instability in metal mines was obtained. Finally, the availability and reasonability of the proposed method were verified by comparing the results obtained with the number of microseismic events detected by the sensors in a metal mine. The proposed model provides a novel approach to dynamic risk assessment in mining, offering a reliable alternative for evaluating complex safety challenges. This method holds substantial potential for its practical application in the assessment and control of rock instability risks in deep metal mines, thereby improving safety and operational efficiency.
Reliable Determination of Pulses and Pulse-Shape Instability in Ultrashort Laser Pulse Trains Using Polarization-Gating and Transient-Grating Frequency-Resolved Optical Gating Using the RANA Approach
Authors: Jafari Rana , Grace Elizabeth , Trebino Rick
Published: February 2025

Devices that measure the presence of instability in the pulse shapes in trains of ultrashort laser pulses do not exist, so this task necessarily falls to pulse-measurement devices, like Frequency-Resolved Optical Gating (FROG) and its variations, which have proven to be a highly reliable class of techniques for measuring stable trains of ultrashort laser pulses. Fortunately, multi-shot versions of FROG have also been shown to sensitively distinguish trains of stable from those of unstable pulse shapes by displaying readily visible systematic discrepancies between the measured and retrieved traces in the presence of unstable pulse trains. However, the effects of pulse-shape instability and algorithm stagnation can be indistinguishable, so a never-stagnating algorithm—even when instability is present—is required and is generally important. In previous work, we demonstrated that our recently introduced Retrieved-Amplitude N-grid Algorithmic (RANA) approach produces highly reliable (100%) pulse-retrieval in the second-harmonic-generation (SHG) version of FROG for thousands of sample trains of pulses with stable pulse shapes. Further, it does so even for trains of unstable pulse shapes and thus both reliably distinguishes between the two cases and provides a rough measure of the degree of instability as well as a reasonable estimate of most typical pulse parameters. Here, we perform the analogous study for the polarization-gating (PG) and transient-grating (TG) versions of FROG, which are often used for higher-energy pulse trains. We conclude that PG and TG FROG, coupled with the RANA approach, also provide reliable indicators of pulse-shape instability. In addition, for PG and TG FROG, the RANA approach provides an even better estimate of a typical pulse in an unstable pulse train than SHG FROG does, even in cases of significant pulse-shape instability.
Optimization of Long Superficial Femoral Artery Percutaneous Transluminal Angioplasty by Intraoperative Doppler Ultrasound
Authors: Vadalà Giuseppe , Roscitano Giuseppe , Virga Vittorio , Castriota Fausto , Pernice Vincenzo , Galassi Alfredo Ruggero , Micari Antonio
Published: February 2025

Background: Residual stenosis and dissections occurring after femoro-popliteal percutaneous transluminal angioplasty (PTA) can compromise vessel patency and should therefore be properly dealt with at the time of the index procedure. Sometimes, the decision to optimize the PTA result may be challenging because it is based on evidence from angiography which, as a bi-dimensional imaging technique, has well-known limitations. Objectives: The objective of this study was to evaluate whether an intraoperative Doppler ultrasound (DUS) assessment of femoro-popliteal lesions that have undergone PTA allows for a more accurate definition of technical success compared to angiography. This was assessed through the identification of those lesions that, despite fulfilling angiographic criteria for technical success, have a functional relevance at DUS. Methods: In this cross-sectional study, data from 62 patients who had undergone femoro-popliteal PTA and intraoperative DUS were retrieved. The procedures were performed at two referral hospitals for peripheral interventions between 2018 and 2019. The selected patients had 48 residual stenoses in the range of 40–50% and 14 grade-C dissections. Results: The mean lesion length was 211 ± 85.2. Angiography-based and DUS-based technical success matched 41 lesions (66%). The remaining 21 lesions, which had abnormal DUS, were successfully optimized by post-dilatation or stenting in 19 cases. Finally, after PTA optimization, DUS-based and angiography-based technical success matched 95% of cases. Conclusions: An intraoperative DUS assessment of intermediate residual stenosis or grade C dissections occurring after PTA of long femoro-popliteal arteries allowed for a more accurate definition of technical success than angiography and guided a further PTA optimization in one-third of the cases.
Is Pain Stronger in Adults or in Adolescents During Semi-Rapid Maxillary Expansion (SRME) and Fixed Appliance (FA) Therapies?
Authors: Kuc-Michalska Małgorzata , Pokucińska Magdalena , Janiszewska-Olszowska Joanna
Published: February 2025

Rapid or semi-rapid maxillary expansion is a well-established method of treating severe transversal maxillary deficiency or transversal dental arch discrepancy. Pain is considered an inevitable undesirable adverse effect of orthodontic treatment. The aim of the present study was to compare pain levels experienced from the semi-RME (SRME) procedure between adults and adolescents and the lower fixed appliance (FA) procedure in the same patients. The study comprised 95 patients (51 adults and 44 adolescents) treated with a bonded acrylic Hass-type expander and the following month (after blocking the screw) with a lower fixed orthodontic appliance. Pain was assessed using a numerical rating scale from 0 to 10. During screw activation, adolescent patients reported a statistically significantly higher median pain level (5.00) compared to adults (3.00) [p = 0.009]. During the fixed appliance treatment, adolescent patients reported similar pain level to adults (4.5 and 4.0, respectively; p = 0.49). Interestingly, the median pain level for SRME in the whole group was 4, and was not different from the fixed appliance treatment sensations. Adolescent females had the highest pain sensation, while adult males had the lowest during both procedures, SRME and FA. The difference between the midpalatal suture opening (appearance of diastema) in adolescents (81.82%) vs. adults (64.71%) was not statistically significant (p = 0.062). The oldest patients with diastema were a 36y-2mo-old man and a 42-year-old woman, whereas the youngest patients without diastema were an 11y-11mo-old girl and a 13y-2mo-old boy. Moreover, all female patients between 30 to 42 years of age (eight women) had presented with diastema. In both age groups, the biggest disadvantage during SRME, reported by 50.53% of all patients (45.45% of adolescents and 54.9% of adults; p = 0.062), were speech problems, whereas only 18.95% of the total group (27.27% of adolescents vs. 11.76% of adults; p = 0.054) considered pain as the main disadvantage. During FA therapy, 32.63% of all patients reported pressure sores as the main disadvantage (29.55% of adolescents and 35.29% of adults; p = 0.551).
Comprehensive Evaluation of Remote Tower Controllers’ Situation Awareness Level Based on the Entropy Weight Method (EWM)–TOPSIS–Gray Relational Analysis Model
Authors: Lu Tingting , Hao Miao , Zhang Zhaoning
Published: February 2025

In recent years, the rapid development of remote tower technology has made it crucial to accurately assess the situational awareness (SA) levels of remote tower controllers. Such an assessment is significant for controller training and remote tower system design. This study employed the SART scale to compare controllers’ SA scores in traditional and remote tower environments. Results revealed significant differences, especially in attention demand and situational understanding. Subsequently, a quantitative analysis of controllers’ perception, understanding, and decision-making abilities was conducted, integrating subjective and objective data. Eye-tracking, heart rate, working memory scales, and communication-coordination scales showed significant results. Experienced controllers had better psychological safety skills, while trainees were more likely to increase vigilance. Moreover, a series of sensitive SA indicators were identified. An evaluation index system was established using the entropy weight method. By calculating the Euclidean distance, Gray relational degree, and comprehensive proximity coefficient, the SA levels of controllers were comprehensively evaluated. The top five important indicators were average blink rate, scan length, average fixation duration, fixation duration, and average pupil diameter. These findings support enhancing air traffic control safety and refining SA assessment for remote tower controllers.
Vestibular Assessment in Infants with Congenital or Early Onset Sensorineural Hearing Loss: Is Neonatal Vestibular Screening Required? A Scoping Review
Authors: Farquhar Lauren , El Refaie Amr
Published: February 2025

Background/Objectives: Children with congenital or early onset sensorineural hearing loss (SNHL) are at a greater risk of vestibular dysfunction (VD), hypothesized to occur from the close embryological relationship between the cochlear and vestibular systems. Even with increasing focus on early detection and rehabilitation through Universal Newborn Hearing Screening (UNHS) programmes in many countries, few studies have focused on the prevalence and feasibility of vestibular assessment in infant populations. The objectives of this review are to 1. identify the prevalence of VD infants with congenital or early onset (<12 months old) SNHL, 2. identify which vestibular assessment tests/protocols are conducted on this population, 3. report sensitivity and specificity values for identified vestibular assessment tests/protocols. Methods: Studies that included infants aged 0–12 months, with congenital or early onset SNHL of any laterality, degree, or configuration, and who underwent any method of vestibular assessment were included. The review adhered to the Joanna Briggs Institute (JBI) guidance and the PRISMA-ScR extension statement. Results: A total of 18 studies were included in the review. All articles reported that infants with congenital or early onset SNHL are at a greater risk of VD, particularly those with bilateral severe–profound SNHL. The cervical vestibular-evoked myogenic potentials (cVEMP) test was the most frequently identified vestibular assessment tool for this age demographic. Conclusions: Results from the included articles coincide with results from literature assessing older paediatric populations. cVEMPs have been reported to be a feasible, sensitive, and specific screening tool in infants with congenital or early onset SNHL. The prevalence of VD in infants with congenital or early onset SNHL justify further investigation on the feasibility of establishing a pathway for vestibular assessment for all infants referred by UNHS programmes.
ChatGPT’s Impact Across Sectors: A Systematic Review of Key Themes and Challenges
Authors: Hussein Hussam , Gordon Madelina , Hodgkinson Cameron , Foreman Robert , Wagad Sumaya
Published: February 2025

This paper critically examines the expanding body of literature on ChatGPT, a transformative AI tool with widespread global adoption. By categorising research into six key themes—sustainability, health, education, work, social media, and energy—it explores ChatGPT’s versatility, benefits, and challenges. The findings highlight its potential to enhance productivity, streamline workflows, and improve access to knowledge while also revealing critical limitations, including high energy consumption, informational inaccuracies, and ethical concerns. The paper underscores the need for robust regulatory frameworks, sustainable AI practices, and interdisciplinary collaboration to optimise benefits while mitigating risks. Future research should focus on improving ChatGPT’s reliability, inclusivity, and environmental sustainability to ensure its responsible integration across diverse sectors.
Fading into Obscurity: Impact of Climate Change on Suitable Habitats for Two Lesser-Known Giant Flying Squirrels (Sciuridae: Petaurista) in Northeastern India
Authors: Abedin Imon , Kamalakannan Manokaran , Mukherjee Tanoy , Choudhury Anwaruddin , Singha Hilloljyoti , Abedin Joynal , Banerjee Dhriti , Kim Hyun-Woo , Kundu Shantanu
Published: February 2025

In recent years, global warming has become a major driver of biodiversity loss, significantly impacting various vertebrate species, including mammals. Consequently, numerous smaller species face extinction risks due to anthropogenic factors as well as inadequate assessments and conservation planning. Thus, this study focuses on two recently described endemic giant flying squirrel species under the Petaurista genus—Petaurista mishmiensis and Petaurista mechukaensis—found in Arunachal Pradesh, India. Using an ensemble species distribution model (SDM), this research delineates suitable habitats for these lesser-known species and evaluates the effects of climate change and habitat fragmentation on these areas. This analysis aims to inform a comprehensive management plan for their conservation. The ensemble model identified suitable habitat patches for the two species, extending beyond their current IUCN-designated ranges in Arunachal Pradesh. Under present conditions, P. mishmiensis has the largest predicted suitable area (9213 sq. km), followed by P. mechukaensis (6754 sq. km). However, future projections reveal alarming habitat losses ranging from 13.45% to 55.86% across the study area. This study also highlights severe habitat fragmentation throughout the state as viable patches for P. mishmiensis are drastically reduced in size, resulting in many being completely lost and the remaining areas being closer together. However, the P. mechukaensis experiences significant disintegration, resulting in numerous smaller, more dispersed patches within Arunachal Pradesh. Hence, to address these challenges, this study recommends several actions such as genetic assessments to confirm evolutionary relationships, evaluations of corridor connectivity, and comprehensive field studies. Furthermore, establishing joint forest conservation committees involving local communities, forest personnel, defense forces, naturalists, and scientists are also encouraged. Ultimately, this research provides critical insights for guiding future field studies across Arunachal Pradesh’s vast landscapes and supports the development of detailed species management plans to protect these endemic flying squirrels.
Nitric Oxide Donor Metallodrug: Single-Inhaler Proposal for Rescue in Acute Allergic Asthma Crises
Authors: Costa Paula Priscila Correia , Waller Stefanie Bressan , Ramos Hálef Herbet , Lopes Neto Belarmino Eugênio , Ribeiro Wesley Lyeverton Correia
Published: February 2025

Allergic asthma is characterized by chronic airway inflammation and recurrent bronchial hyperreactivity, highlighting the need for rapid therapeutic interventions during acute crises. This study aimed to assess the potential of a single-dose administration of the ruthenium nitrosyl complex cis-[Ru(bpy)2(2-MIM)(NO)](PG6)3 (named as FOR811A) as a fast-acting treatment in a murine model of allergic asthma. Female Swiss mice were sensitized with ovalbumin for the induction of asthma and subjected to inhalation challenges. The experimental groups included controls and ovalbumin-sensitized mice receiving FOR811A (0.75 mg/kg) or saline (NaCl 0.9%), both by gavage. Lung tissues were collected for analyses of oxidative damage (nitrite/nitrate and GSH), inflammatory markers (myeloperoxidase, IL-1β, and IL-4), and histological assessment. The results showed that, while FOR811A did not significantly reduce oxidative damage or overall inflammation, it effectively decreased IL-4 levels, indicating a modulation of the Th2 immune response without affecting IL-1β levels (Th1 response). These findings suggest that a single-dose administration of FOR811A may provide a rapid therapeutic effect in allergic asthma crises by promoting smooth muscle relaxation and modulating immune responses. Further research is warranted to explore its clinical utility as a fast-acting rescue medication for acute asthma management.
Biology, Ecology and Management of Aquatic Macrophytes and Algae (Volume I)
Authors: Liu Jinlin , Liu Wei , Zhao Shuang
Published: February 2025

Aquatic macrophytes and algae constitute essential components of aquatic ecosystems, fulfilling diverse and critical roles in sustaining ecological integrity and equilibrium [...]
Evaluation of Biocompatible Materials for Enhanced Mesenchymal Stem Cell Expansion: Collagen-Coated Alginate Microcarriers and PLGA Nanofibers
Authors: Jaime-Rodríguez Manuel , Del Prado-Audelo María Luisa , Sosa-Hernández Norma Angélica , Anaya-Trejo Dulce Patricia , Villarreal-Gómez Luis Jesús , Cabrera-Ramírez Ángel Humberto , Ruiz-Aguirre Jesus Augusto , Núñez-Tapia Israel , Puskar Marek , Marques dos Reis Emily , Letasiova Silvia , Chávez-Santoscoy Rocío Alejandra
Published: February 2025

Mesenchymal stem cells (MSCs) hold significant potential in regenerative medicine, tissue engineering, and cultivated meat production. However, large-scale MSC production is limited by their need for surface adherence during growth. This study evaluates two biocompatible materials—collagen-coated alginate microcarriers and polylactic-co-glycolic acid (PLGA) nanofibers—as novel growth substrates to enhance MSC proliferation. Physicochemical characterization confirmed successful collagen integration on both materials. In vitro, bone marrow-derived MSCs (bmMSCs) cultured on collagen-coated alginate microcarriers exhibited significantly enhanced growth compared to commercial microcarriers, while PLGA nanofibers supported bmMSC growth comparable to traditional growth surfaces. Scanning Electron Microscopy (SEM) revealed that bmMSCs adhered not only to the surface but also grew within the porous structure of the alginate microcarriers. Mycoplasma testing confirmed that the bmMSCs were free from contamination. Both materials were assessed for biocompatibility using ISO-10993 guidelines, demonstrating no skin or ocular irritation, supporting their potential for in situ applications in clinical and therapeutic settings. This study highlights the promise of collagen-coated alginate microcarriers and PLGA nanofibers for scalable MSC production, offering efficient, biocompatible alternatives to traditional growth surfaces in regenerative medicine and cultivated meat manufacturing. Future research should focus on optimizing these materials for larger-scale production and exploring specific applications in therapeutic and food sectors.
MRI T2 Mapping of Dorsal Root Ganglia Reveals Increased T2 Relaxation Time in Classical Fabry Disease
Authors: Weiner Simon , Perleth Sarah , Kampf Thomas , Lau Kolja , Hessenauer Florian , Homola György , Nordbeck Peter , Üçeyler Nurcan , Sommer Claudia , Pham Mirko , Schindehütte Magnus
Published: February 2025

Background/Objectives: Fabry disease (FD) is a rare X-linked lysosomal storage disorder characterised by progressive glycolipid accumulation affecting multiple organs, including the peripheral nervous system. The dorsal root ganglia (DRG) play a key role in Fabry-related neuropathy, but non-invasive biomarkers of DRG involvement and their association with overall disease severity remain limited. This study evaluated lumbosacral DRG T2 relaxation time (DRG-T2) in FD patients as a potential imaging biomarker of FD severity. Methods: In a prospective, single-centre study, 80 genetically confirmed FD patients underwent 3T MRI with quantitative T2 mapping of the lumbosacral DRG. DRG-T2 was analysed in relation to sex, genetic subtype and Fabry-specific biomarkers. Results: Results showed that DRG-T2 was higher in patients with classical FD mutations than in those with nonclassical mutations (p = 0.03). Furthermore, DRG-T2 showed a negative correlation with body weight (ρ = −0.31, p = 0.005) and BMI (ρ = −0.32, p = 0.004), while no associations were found with lyso-Gb3 levels or alpha-galactosidase A activity. The inter-rater and test–retest reliability of DRG-T2 were good to excellent (ICC = 0.76 and 0.89, respectively). Conclusions: These results demonstrate DRG-T2 as a marker of neuronal involvement, making it a strong and reliable imaging biomarker of disease severity in FD. However, future studies need to correlate its changes with clinical and histological studies.
The Impact of Walking on BDNF as a Biomarker of Neuroplasticity: A Systematic Review
Authors: Khalil Mohamed Hesham
Published: February 2025

Background/Objectives: The brain-derived neurotrophic factor (BDNF) is a critical exercise-induced modulator of various neuroplasticity processes, including adult hippocampal neurogenesis. Environmental affordance for physical activity is a novel theory that aims to increase the BDNF through walking or climbing stairs, stimulated by the urban and interior environment. In a systematic review, this paper explores the association between walking, as a structured or free-living form of physical activity, and changes in the BDNF in humans with healthy locomotion. Method: A systematic review with a registered protocol, INPLASY2024110093, and following the PRISMA guidelines, includes English-language original research articles on adult and older adult human subjects who are locomotor-healthy, studies on walking as a structured exercise or free-living physical activity that is presented in a non-combined intervention, and must report changes in the BDNF as a dependent variable. The search was conducted using three databases: PubMed, Web of Science, and Scopus, resulting in 21 eligible studies. Results: This systematic review finds that the impact of walking on the BDNF is evidenced, but subject to moderate to high intensities in single bouts. At the same time, the long-term effects are yet to be fully understood, potentially due to the uptake of the BDNF for functional brain improvements, neuroplasticity processes, or muscle repair, instead of an accumulation of the BDNF itself, yet still confirm the important role of the BDNF for neurosustainability. Age and environmental factors such as heat are also found to affect the increase in the BDNF. The narrative synthesis provides elaborate explanations for understanding those complex dynamics before reaching future conclusions on the impact of walking or environmental affordance for physical activity on the changes in the BDNF concentrations. Conclusions: This systematic review highlights the potential role played by moderate- and high-intensity walking as a lifestyle intervention that can be utilised through the built environment to promote adaptive brain changes, through the sustainable regulation of the BDNF.
Does Lumbar Puncture Still Have Clinical Value for Patients with Amyotrophic Lateral Sclerosis?
Authors: Ginanneschi Federica , Casali Stefania , Cioni Chiara , Righi Delia , Emmanuello Emanuele , Toccaceli Cecilia , Plantone Domenico , De Stefano Nicola
Published: February 2025

Background: The relationship between routine cerebrospinal fluid (CSF) testing and clinical and prognostic data in amyotrophic lateral sclerosis (ALS) remains unclear. Additionally, biochemical data have never been correlated with markers of neurodegeneration. The purpose of this study is to determine whether lumbar puncture may still have clinical utility in ALS. Methods: We collected the CSF profiles of 140 ALS subjects. CSF protein, albumin, IgG, IgG index, albumin quotient (QAlb), t-tau, p-tau, and Aβ42 were analyzed. Results: Approximately one-quarter of ALS patients had elevated levels of protein, albumin, and QAlb in the CSF, but these were not associated with clinical or survival data. Among the neurodegeneration markers, the percentage of patients with abnormal values ranged from 26.3% to 35.4%. The p-tau/t-tau ratio and Aβ42 were correlated with both the ALS progression rate and the time from diagnosis to death. Aβ42 was the prognostic marker most strongly associated with survival. Conclusions: The lack of correlation between biochemical CSF findings and the clinical and/or prognostic status of ALS suggests that these markers have no clinical value. However, neurodegeneration markers that are easily measurable in clinical laboratories, particularly Aβ42, may be useful at the time of diagnosis for predicting ALS survival and progression rate.