bluuebunny/crossref_metadata_2025 huggingface dataset:
Recent Approaches for Chemical Speciation and Analysis by Electrospray Ionization (ESI) Mass Spectrometry
Authors: Indelicato Serena , Bongiorno David , Ceraulo Leopoldo
Published: January 2021

In recent years, the chemical speciation of several species has been increasingly monitored and investigated, employing electrospray ionization mass spectrometry (ESI-MS). This soft ionization technique gently desolvates weak metal–ligand complexes, taking them in the high vacuum sectors of mass spectrometric instrumentation. It is, thus, possible to collect information on their structure, energetics, and fragmentation pathways. For this reason, this technique is frequently chosen in a synergistic approach to investigate competitive ligand exchange-adsorption otherwise analyzed by cathodic stripping voltammetry (CLE-ACSV). ESI-MS analyses require a careful experimental design as measurement may face instrumental artifacts such as ESI adduct formation, fragmentation, and sometimes reduction reactions. Furthermore, ESI source differences of ionization efficiencies among the detected species can be misleading. In this mini-review are collected and critically reported the most recent approaches adopted to mitigate or eliminate these limitations and to show the potential of this analytical technique.
Acute Changes in Carotid-Femoral Pulse-Wave Velocity Are Tracked by Heart-Femoral Pulse-Wave Velocity
Authors: Stone Keeron , Fryer Simon , Faulkner James , Meyer Michelle L. , Zieff Gabriel , Paterson Craig , Burnet Kathryn , Kelsch Elizabeth , Credeur Daniel , Lambrick Danielle , Stoner Lee
Published: January 2021

Background: Carotid-femoral pulse-wave velocity (cfPWV) is the reference standard measure of central arterial stiffness. However, it requires assessment of the carotid artery, which is technically challenging, and subject-level factors, including carotid artery plaque, may confound measurements. A promising alternative that overcomes these limitations is heart-femoral PWV (hfPWV), but it is not known to what extent changes in cfPWV and hfPWV are associated.

Objectives: To determine, (1) the strength of the association between hfPWV and cfPWV; and (2) whether change in hfPWV is associated with change in cfPWV when central arterial stiffness is perturbed.

Methods: Twenty young, healthy adults [24.0 (SD: 3.1) years, 45% female] were recruited. hfPWV and cfPWV were determined using Doppler ultrasound at baseline and following a mechanical perturbation in arterial stiffness (120 mmHg thigh occlusion). Agreement between the two measurements was determined using mixed-effects regression models and Bland-Altman analysis.

Results: There was, (1) strong (ICC > 0.7) agreement between hfPWV and cfPWV (ICC = 0.82, 95%CI: 0.69, 0.90), and, (2) very strong (ICC > 0.9) agreement between change in hfPWV and cfPWV (ICC = 0.92, 95%CI: 0.86, 0.96). cfPWV was significantly greater than hfPWV at baseline and during thigh occlusion (both P < 0.001). Inspection of the Bland-Altman plot, comparing cfPWV and corrected hfPWV, revealed no measurement magnitude bias.

Discussion: The current findings indicate that hfPWV and cfPWV are strongly associated, and that change in cfPWV is very strongly associated with change in hfPWV. hfPWV may be a simple alternative to cfPWV in the identification of cardiovascular risk in clinical and epidemiological settings.
Genetics and Clinical Features of Noncompaction Cardiomyopathy in the Fetal Population
Authors: Sun Hairui , Hao Xiaoyan , Wang Xin , Zhou Xiaoxue , Zhang Ye , Liu Xiaowei , Han Jiancheng , Gu Xiaoyan , Sun Lin , Zhao Ying , Yi Tong , Zhang Hongjia , He Yihua
Published: January 2021

Objectives: Noncompaction Cardiomyopathy (NCCM) has been classified as primary genetic cardiomyopathy and has gained increasing clinical awareness; however, little is known about NCCM in the fetal population. We aimed to investigate the clinical characteristics and genetic spectrum of a fetal population with NCCM.

Methods: We retrospectively reviewed all fetuses with a prenatal diagnosis of NCCM at a single center between October 2010 and December 2019. These cases were investigated for gestational age at diagnosis, gender, left or biventricular involvement, associated cardiac phenotypes, outcomes, and genetic testing data.

Results: We identified 37 fetuses with NCCM out of 49,898 fetuses, indicating that the incidence of NCCM in the fetal population was 0.07%. Of the 37 fetuses, 26 were male, ten were female and one was of unknown gender. NCCM involvement biventricle is the most common (n = 16, 43%), followed by confined to the left ventricle (n = 14, 38%). Nineteen (51%) had additional congenital heart defects, with right-sided lesions being the most common (n = 14, 74%), followed by ventricular septal defects (n = 10, 53%). Hydrops fetalis was present in 12 cases (32%), of which four were atypical (pericardial effusion only). Sequencing analysis was performed at autopsy (n = 19) or postnatally (n = 1) on 20 fetuses. Of the 20 fetuses undergoing copy number variation sequencing and whole-exome sequencing, nine (47%) had positive genetic results, including one with a pathogenic copy number variant and eight with pathogenic/likely pathogenic variants. Non-sarcomere gene mutations accounted for the vast majority (n = 7). In contrast, sarcomere gene mutations occurred in only one case (TPM1), and no mutations were identified in the three most common sarcomere genes (MYH7, TTN, and MYBPC3) of pediatric and adult patients. Pathogenic/likely pathogenic variants were significantly more frequent in fetuses with congenital heart defects than those without congenital heart defects.

Conclusions: Our data demonstrate that fetal NCCM is a unique entity. Compared with pediatric and adult NCCM, fetal NCCM is more prone to biventricle involvement, more likely to be complicated with congenital heart defects, and has a distinct genetic spectrum.
Characteristics of Blood Metabolic Profile in Coronary Heart Disease, Dilated Cardiomyopathy and Valvular Heart Disease Induced Heart Failure
Authors: Liu Chang , Li Ruihua , Liu Yang , Li Zhenguo , Sun Yujiao , Yin Peiyuan , Huang Rihong
Published: January 2021

Purpose: Metabolic impairment is one key contributor to heart failure (HF) pathogenesis and progression. The major causes of HF, coronary heart disease (CHD), dilated cardiomyopathy (DCM), and valvular heart disease (VHD) remains poorly characterized in patients with HF from the view of metabolic profile. We sought to determine metabolic differences in CHD-, VHD-, and DCM-induced HF patients and identify significantly altered metabolites and their correlations.

Procedure: In this study, a total of 96 HF cases and 97 controls were enrolled. The contents of 23 amino acids and 26 carnitines in fasting plasma were measured by a targeted liquid chromatography and mass spectrometry (LC-MS) approach.

Results: Nine metabolites (Histidine, Arginine, Citrulline, Glutamine, Valine, hydroxyhexadecenyl-carnitine, acylcarnitine C22, hydroxytetradecanoyl-carnitine, and carnitine) were found to be related with the occurrence of HF. Arginine, Glutamine and hydroxytetradecanoyl-carnitine could effectively distinguish CHD and DCM patients, and hydroxytetradecanoyl-carnitine and aspartic acid were able to classify CHD and VHD cohorts.

Conclusion: This study indicated that circulating amino acids and long-chain acylcarnitine levels were closely associated with progression of heart failure. Monitoring these metabolic alterations by LC-MS may help the differentiation of CHD, VHD, and DCM in the early stage, and provide new diagnostics targets or therapeutic interventions.
How Does Indian Monsoon Regulate the Northern Hemisphere Stationary Wave Pattern?
Authors: Son Jun-Hyeok , Seo Kyong-Hwan , Son Seok-Woo , Cha Dong-Hyun
Published: January 2021

The Northern Hemisphere summer climate isstrongly affected by a circumglobal stationary Rossby wave train, which can be manifested by the first EOF mode of the geopotential height at 200 hPa. Interannual variation of this Northern Hemisphere wave (NHW) pattern has a significant impact on remarkably warm surface temperature anomalies over the North Atlantic, Northeast Europe, East Asia to Central-North Pacific, and America, particularly in 2018 and 2010. The NHW pattern is likely generated by atmospheric diabatic heating and vorticity forcing: diabatic heating is mainly confined in the Indian summer monsoon (ISM) precipitation region, whereas the anti-cyclonic vorticity forcing is distributed in the globe. The ISM is a well-known diabatic heat source; however, the main source of vorticity forcing has not been established. In general, the tropical vorticity anomaly comes from diabatic heating-induced atmospheric waves and randomly generated inherent internal waves. The linear baroclinic model experiment reveals that the NHW pattern can be generated by the westward propagating tropical waves generated by the ISM diabatic heat forcing.
Diffuse Tectonic Deformation in the Drum Mountains Fault Zone, Utah, USA: Testing the Utility of Legacy Aerial Photograph-Derived Topography
Authors: Stahl Timothy A. , Niemi Nathan A. , Delano Jaime E. , Wolfe Franklin D. , Bunds Michael P. , Howell Andrew
Published: January 2021

The Basin and Range province in the western United States hosts numerous low-slip-rate normal faults with diffuse and subtle surface expressions. Legacy aerial photographs, widely available across the region, can be used to generate high-resolution digital elevation models of these previously uncharacterized fault systems. Here, we test the limits and utility of aerial photograph-derived elevation products on the Drum Mountains fault zone—a virtually unstudied and enigmatic fault system in the eastern Basin and Range province of central Utah. We evaluate a new 2-m digital surface model produced from aerial photographs against other remotely sensed and field survey data and assess the various factors that contribute to noise, artifacts, and distortions. Despite some challenges, the new elevation model captures the complex array of cross-cutting fault scarps well. We demonstrate that the fault zone has variable net east- or west-down sense of displacement across a c. 8-km-wide zone of antithetic and synthetic traces. Optically stimulated luminescence ages and scarp profiles are used to constrain net extension rates across two transects and reveal that the Drum Mountains fault zone has average extension rates of c. 0.1–0.4 mm yr−1over the last c. 35 ka. These rates are both faster than previously estimated and faster than most other faults in the region, and could be an order of magnitude higher if steep faults at the surface sole into a detachment at depth. Several models have been proposed for local and regional faulting at depth, but our data show that the offsets, rates, and geometries of faulting can be generated by the reactivation of pre-existing, cross-cutting faults in a structurally complex zone between other fault systems. This study highlights how legacy aerial-photograph-derived elevation products, in lieu of other high-resolution topographic datasets, can be used to study active faults, especially in remote regions where diffuse deformation would otherwise remain undetected.
Stratigraphy and Chronology of Sodicho Rockshelter – A New Sedimentological Record of Past Environmental Changes and Human Settlement Phases in Southwestern Ethiopia
Authors: Hensel Elena A. , Vogelsang Ralf , Noack Tom , Bubenzer Olaf
Published: January 2021

The preservation of archaeological remains and environmental information in a sediment accumulation can vary in caves and rockshelters, depending on external climatic conditions, and the circumstances within the shelter. Several sediment stratigraphies in the Horn of Africa are characterized by erosion layers, discordances and chronological gaps, that create uncertainties about the impact of climatic and environmental shifts on human settlements. Archaeological sites in Ethiopia that preserve information about human occupation during the Upper Pleistocene and Holocene often deal with major gaps during a period corresponding to MIS 2. In this study we present the first results of sedimentological, geochemical analyses and radiocarbon dating at Sodicho Rockshelter (1930 m above sea level) that provide evidence on high altitude settlement during this mentioned chronostratigraphic gap and subsequent time slices. This new archaeological site in the southwestern Ethiopian Highlands hosts a 2-m-long sediment record. So far, a stratigraphy has been excavated that dates back to ∼27 ka, including several settlement phases of Late Pleistocene and Holocene hunter-gatherers and providing information on environmental changes. A multiproxy approach was chosen to establish a first general stratigraphy of the site and to disentangle the sediment composition as well as site formation processes. The results suggest a variation of allochthonous and autochthonous geogenic deposits, and anthropogenic accumulation processes. With the help of radiocarbon dating, anthropogenic layers were dated covering the arid Last Glacial Maximum (LGM, ∼21 ± 2 ka). The occupation phases were interrupted in cause of environmental changes. The most prominent is the accumulation of reddish, archaeological sterile deposits that can be chronologically associated with the African Humid Period (AHP, ∼15–5 ka BP). Geochemical records point to dry spells within this humid phase, suggesting correlations with regional climate signals of lacustrine sediments. These sediment accumulations of past wet conditions are covered by alternating layers of Holocene volcanic fallout and sediments with preserved cultural material. Our study provides a preliminary impression of still poorly understood time periods of human occupation in the southwestern Ethiopian Highlands. The data obtained from Sodicho Rockshelter could validate the current state of knowledge and partially reduce the chronostratigraphic gap.
Strategies for Hydrogen-Enriched Methane Flameless Combustion in a Quasi-Industrial Furnace
Authors: Amaduzzi Ruggero , Ferrarotti Marco , Parente Alessandro
Published: January 2021

In this present work, simulations of 20 kW furnace were carried out with hydrogen-enriched methane mixtures, to identify optimal geometrical configurations and operating conditions to operate in flameless combustion regime. The objective of this work is to show the advantages of flameless combustion for hydrogen-enriched fuels and the limits of current typical industrial designs for these mixtures. The performances of a semi-industrial combustion chamber equipped with a self-recuperative flameless burner are evaluated with increasing H2 concentrations. For highly H2-enriched mixtures, typical burners employed for methane appear to be inadequate to reach flameless conditions. In particular, for a typical coaxial injector configuration, an equimolar mixture of hydrogen and methane represents the limit for hydrogen enrichment. To achieve flameless conditions, different injector geometries and configuration were tested. Fuel dilution with CO2 and H2O was also investigated. Dilution slows the mixing process, consequently helping the transition to flameless conditions. CO2, and H2O are typical products of hydrogen generation processes, therefore their use in fuel dilution is convenient for industrial applications. Dilution thus allows the use of greater hydrogen percentages in the mixture.
Viral Infections in HSCT: Detection, Monitoring, Clinical Management, and Immunologic Implications
Authors: Annaloro Claudio , Serpenti Fabio , Saporiti Giorgia , Galassi Giulia , Cavallaro Francesca , Grifoni Federica , Goldaniga Maria , Baldini Luca , Onida Francesco
Published: January 2021

In spite of an increasing array of investigations, the relationships between viral infections and allogeneic hematopoietic stem cell transplantation (HSCT) are still controversial, and almost exclusively regard DNA viruses. Viral infections per se account for a considerable risk of morbidity and mortality among HSCT recipients, and available antiviral agents have proven to be of limited effectiveness. Therefore, an optimal management of viral infection represents a key point in HSCT strategies. On the other hand, viruses bear the potential of shaping immunologic recovery after HSCT, possibly interfering with control of the underlying disease and graft-versus-host disease (GvHD), and eventually with HSCT outcome. Moreover, preliminary data are available about the possible role of some virome components as markers of immunologic recovery after HSCT. Lastly, HSCT may exert an immunotherapeutic effect against some viral infections, notably HIV and HTLV-1, and has been considered as an eradicating approach in these indications.
CD8+ T-Cell Repertoire in Human Leukocyte Antigen Class I-Mismatched Alloreactive Immune Response
Authors: Bettens Florence , Calderin Sollet Zuleika , Buhler Stéphane , Villard Jean
Published: January 2021

In transplantation, direct allorecognition is a complex interplay between T-cell receptors (TCR) and HLA molecules and their bound peptides expressed on antigen-presenting cells. In analogy to HLA mismatched hematopoietic stem cell transplantation (HSCT), the TCR CDR3β repertoires of alloreactive cytotoxic CD8+ responder T cells, defined by the cell surface expression of CD137 and triggered in vitro by HLA mismatched stimulating cells, were analyzed in different HLA class I mismatched combinations. The same HLA mismatched stimulatory cells induced very different repertoires in distinct but HLA identical responders. Likewise, stimulator cells derived from HLA identical donors activated CD8+ cells expressing very different repertoires in the same mismatched responder. To mimic in vivo inflammation, expression of HLA class l antigens was upregulated in vitro on stimulating cells by the inflammatory cytokines TNFα and IFNβ. The repertoires differed whether the same responder cells were stimulated with cells treated or not with both cytokines. In conclusion, the selection and expansion of alloreactive cytotoxic T-cell clonotypes expressing a very diverse repertoire is observed repeatedly despite controlling for HLA disparities and is significantly influenced by the inflammatory status. This makes prediction of alloreactive T-cell repertoires a major challenge in HLA mismatched HSCT.
Bottom Trawling Threatens Future Climate Refugia of Rhodoliths Globally
Authors: Fragkopoulou Eliza , Serrão Ester A. , Horta Paulo A. , Koerich Gabrielle , Assis Jorge
Published: January 2021

Climate driven range shifts are driving the redistribution of marine species and threatening the functioning and stability of marine ecosystems. For species that are the structural basis of marine ecosystems, such effects can be magnified into drastic loss of ecosystem functioning and resilience. Rhodoliths are unattached calcareous red algae that provide key complex three-dimensional habitats for highly diverse biological communities. These globally distributed biodiversity hotspots are increasingly threatened by ongoing environmental changes, mainly ocean acidification and warming, with wide negative impacts anticipated in the years to come. These are superimposed upon major local stressors caused by direct destructive impacts, such as bottom trawling, which act synergistically in the deterioration of the rhodolith ecosystem health and function. Anticipating the potential impacts of future environmental changes on the rhodolith biome may inform timely mitigation strategies integrating local effects of bottom trawling over vulnerable areas at global scales. This study aimed to identify future climate refugia, as regions where persistence is predicted under contrasting climate scenarios, and to analyze their trawling threat levels. This was approached by developing species distribution models with ecologically relevant environmental predictors, combined with the development of a global bottom trawling intensity index to identify heavily fished regions overlaying rhodoliths. Our results revealed the importance of light, thermal stress and pH driving the global distribution of rhodoliths. Future projections showed poleward expansions and contractions of suitable habitats at lower latitudes, structuring cryptic depth refugia, particularly evident under the more severe warming scenario RCP 8.5. Our results suggest that if management and conservation measures are not taken, bottom trawling may directly threaten the persistence of key rhodolith refugia. Since rhodoliths have slow growth rates, high sensitivity and ecological importance, understanding how their current and future distribution might be susceptible to bottom trawling pressure, may contribute to determine the fate of both the species and their associated communities.
Marine Important Bird and Biodiversity Areas for Penguins in Antarctica, Targets for Conservation Action
Authors: Handley Jonathan , Rouyer Marie-Morgane , Pearmain Elizabeth J. , Warwick-Evans Victoria , Teschke Katharina , Hinke Jefferson T. , Lynch Heather , Emmerson Louise , Southwell Colin , Griffith Gary , Cárdenas César A. , Franco Aldina M. A. , Trathan Phil , Dias Maria P.
Published: January 2021

Global targets for area-based conservation and management must move beyond threshold-based targets alone and must account for the quality of such areas. In the Southern Ocean around Antarctica, a region where key biodiversity faces unprecedented risks from climate change and where there is a growing demand to extract resources, a number of marine areas have been afforded enhanced conservation or management measures through two adopted marine protected areas (MPAs). However, evidence suggests that additional high quality areas could benefit from a proposed network of MPAs. Penguins offer a particular opportunity to identify high quality areas because these birds, as highly visible central-place foragers, are considered indicator species whose populations reflect the state of the surrounding marine environment. We compiled a comprehensive dataset of the location of penguin colonies and their associated abundance estimates in Antarctica. We then estimated the at-sea distribution of birds based on information derived from tracking data and through the application of a modified foraging radius approach with a density decay function to identify some of the most important marine areas for chick-rearing adult penguins throughout waters surrounding Antarctica following the Important Bird and Biodiversity Area (IBA) framework. Additionally, we assessed how marine IBAs overlapped with the currently adopted and proposed network of key management areas (primarily MPAs), and how the krill fishery likely overlapped with marine IBAs over the past five decades. We identified 63 marine IBAs throughout Antarctic waters and found that were the proposed MPAs to be adopted, the permanent conservation of high quality areas for penguin species would increase by between 49 and 100% depending on the species. Furthermore, our data show that, despite a generally contracting range of operation by the krill fishery in Antarctica over the past five decades, a consistently disproportionate amount of krill is being harvested within marine IBAs compared to the total area in which the fishery operates. Our results support the designation of the proposed MPA network and offer additional guidance as to where decision-makers should act before further perturbation occurs in the Antarctic marine ecosystem.
Analysis on the Dynamic Wave Attenuation Properties of Metaconcrete Considering a Quasi-Random Arrangement of Inclusions
Authors: Briccola Deborah , Pandolfi Anna
Published: January 2021

The mitigation properties of metaconcrete cast with two types of resonant inclusions are assessed through wave transmission tests. Three cylindric metaconcrete specimens of regular size (20 cm height, 10 cm diameter), containing an equal number of different type of inclusions disposed in a semi-regular lattice, are tested in the longitudinal direction within the sonic range of frequencies. Inclusions, bi-material spheres consisting of a heavy core coated with a soft material, are characterized by a resonant behavior, evaluated numerically with a finite element modal analysis of a unit metaconcrete cell. Each metaconcrete specimen contains six layers consisting of six engineered aggregates of different type. Inclusions are disposed by rotating each layer with respect to the adjacent ones, as so as to create a pseudo-random arrangement. Specimens are excited by a sinusoidal signal of linearly growing frequency, sweeping a range centered at the translational eigenfrequency of the resonant inclusion. A standard plain concrete specimen is used as reference to define a transmissibility coefficient, that facilitates the quantification of the attenuation properties. With respect to plain concrete, all metaconcrete specimens show a marked (up to 80–90%) attenuation of the transmitted signal in proximity of the numerically estimated eigenfrequency of the inclusion. The intensity of the attenuation is weakly dependent on the type of the inclusion, while the frequency where the attenuation is observed depends markedly on the inclusion type. As a very positive quality in the view of practical applications, experimental results confirm that the attenuation effectiveness of metaconcrete is not related to the ordered microstructural arrangement.
Sphingolipidomics in Translational Sepsis Research–Biomedical Considerations and Perspectives
Authors: Claus Ralf A. , Graeler Markus H.
Published: January 2021

Scientific Background: Sphingolipids are a highly diverse group of lipids with respect to physicochemical properties controlling either structure, distribution, or function, all of them regulating cellular response in health and disease. Mass spectrometry, on the other hand, is an analytical technique characterizing ionized molecules or fragments thereof by mass-to-charge ratios, which has been prosperingly developed for rapid and reliable qualitative and quantitative identification of lipid species. Parallel to best performance of in-depth chromatographical separation of lipid classes, preconditions of precise quantitation of unique molecular species by preprocessing of biological samples have to be fulfilled. As a consequence, “lipid profiles” across model systems and human individuals, esp. complex (clinical) samples, have become eminent over the last couple of years due to sensitivity, specificity, and discriminatory capability. Therefore, it is significance to consider the entire experimental strategy from sample collection and preparation, data acquisition, analysis, and interpretation.

Areas Covered: In this review, we outline considerations with clinical (i.e., human) samples with special emphasis on sample handling, specific physicochemical properties, target measurements, and resulting profiling of sphingolipids in biomedicine and translational research to maximize sensitivity and specificity as well as to provide robust and reproducible results. A brief commentary is also provided regarding new insights of “clinical sphingolipidomics” in translational sepsis research.

Expert Opinion: The role of mass spectrometry of sphingolipids and related species (“sphingolipidomics”) to investigate cellular and compartment-specific response to stress, e.g., in generalized infection and sepsis, is on the rise and the ability to integrate multiple datasets from diverse classes of biomolecules by mass spectrometry measurements and metabolomics will be crucial to fostering our understanding of human health as well as response to disease and treatment.
Students’ Experiences of Working With a Socio-Scientific Issues-Based Curriculum Unit Using Role-Playing to Negotiate Antibiotic Resistance
Authors: Sagmeister Konstantin J. , Schinagl Christoph W. , Kapelari Suzanne , Vrabl Pamela
Published: January 2021

The emergence and widespread of antibiotic-resistant pathogenic microorganisms are of great individual and societal relevance. Due to the complex and multilayered nature of the topic, antibiotic resistance (ABR) is the object of concern for several scientific fields, such as microbiology or medicine, and encompasses a broad range of political, economic, and social aspects. Thus, the issue related to antibiotic-resistant bacterial diseases offers an excellent platform for designing and implementing the teaching and learning of socio-scientific issues (SSI). We created a SSI-based curriculum unit for use in secondary science classrooms by developing a collaborative partnership between education researchers and microbiologists. This classroom environment allows students to explore and negotiate ABR as a societal and scientific phenomenon. For this purpose, we leveraged role-playing within the SSI-based unit as a productive context for engaging students in learning opportunities that provide multiple perspectives on ABR and the complex interplay of its accelerators. This case-based paper describes Austrian school students’ experiences from their participation in a SSI-embedded role-playing classroom environment and subsequent activities that included a mini congress with a poster presentation and a panel discussion. An open-ended questionnaire-based assessment tool was used to examine the situational characteristics of the students’ work. To assess students’ contributions, we applied a qualitative content analysis design and identified cognitive and affective outcomes. The students’ learning experiences demonstrate that they considered the content – the social complexities of antibiotic-resistant bacteria and associated diseases – exciting and very topical. The students perceived that learning about ABR is relevant for their future and involves both individual and societal responsibility for action. Although the curriculum unit and its assignments were described as labor-intensive, it became apparent that the role-playing setting has the potential to inform students about multiple stakeholder positions concerning ABR. Concerning the promotion of science practices, almost all students claimed that they learned to organize, analyze, evaluate, and present relevant information. Moreover, the students affirmed that they learned to argue from the perspective of their assigned roles. However, the students did not clarify whether they learned more through this SSI-based classroom instruction than through conventional science teaching approaches.
Population Genetic Analysis of the Theileria annulata Parasites Identified Limited Diversity and Multiplicity of Infection in the Vaccine From India
Authors: Roy Sonti , Bhandari Vasundhra , Barman Madhumanti , Kumar Pankaj , Bhanot Vandna , Arora Jaspreet Singh , Singh Satparkash , Sharma Paresh
Published: January 2021

Background:Apicomplexan parasiteTheileria annulatacauses significant economic loss to the livestock industry in India and other tropical countries. In India, parasite control is mainly dependent on the live attenuated schizont vaccine and the drug buparvaquone. For effective disease control, it is essential to study the population structure and genetic diversity of theTheileria annulatafield isolates and vaccine currently used in India.

Methodology/Results:A total of 125T. annulataisolates were genotyped using 10 microsatellite markers from four states belonging to different geographical locations of India. Limited genetic diversity was observed in the vaccine isolates when compared to the parasites in the field; a level of geographical substructuring was evident in India. The number of genotypes observed per infection was highest in India when compared to other endemic countries, suggesting high transmission intensity and abundance of ticks in the country. A reduced panel of four markers can be used for future studies in these for surveillance of theT. annulataparasites in India.

Conclusion:High genetic variation between the parasite populations in the country suggests their successful spread in the field and could hamper the disease control programs. Our findings provide the baseline data for the diversity and population structure ofT. annulataparasites from India. The low diversity in the vaccine advocates improving the current vaccine, possibly by increasing its heterozygosity. The reduced panel of the markers identified in this study will be helpful in monitoring parasite and its reintroduction afterTheileriaeradication.
The Cyclic Oligoadenylate Signaling Pathway of Type III CRISPR-Cas Systems
Authors: Huang Fengtao , Zhu Bin
Published: January 2021

Type III CRISPR-Cas systems, which are widespread in both bacteria and archaea, provide immunity against DNA viruses and plasmids in a transcription-dependent manner. Since an unprecedented cyclic oligoadenylate (cOA) signaling pathway was discovered in type III systems in 2017, the cOA signaling has been extensively studied in recent 3 years, which has expanded our understanding of type III systems immune defense and also its counteraction by viruses. In this review, we summarized recent advances in cOA synthesis, cOA-activated effector protein, cOA signaling-mediated immunoprotection, and cOA signaling inhibition, and highlighted the crosstalk between cOA signaling and other cyclic oligonucleotide-mediated immunity discovered very recently.
Terroir Is the Main Driver of the Epiphytic Bacterial and Fungal Communities of Mango Carposphere in Reunion Island
Authors: Taîbi Ahmed , Rivallan Ronan , Broussolle Véronique , Pallet Dominique , Lortal Sylvie , Meile Jean-Christophe , Constancias Florentin
Published: January 2021

The diversity of both bacterial and fungal communities associated with mango surface was explored using a metabarcoding approach targeting fungal ITS2 and bacterial 16S (V3-V4) genomic regions. Fruits were collected in Reunion Island from two different orchards according to a sampling method which allowed the effect of several pre-harvest factors such as geographical location (terroir), cultivars, fruit parts, tree position in the plot, fruit position on the tree (orientation and height), as well as the harvest date to be investigated. A total of 4,266,546 fungal and 2,049,919 bacterial reads were recovered then respectively assigned to 3,153 fungal and 24,087 to bacterial amplicon sequence variants (ASVs). Alpha and beta diversity, as well as differential abundance analyses revealed variations in both bacterial and fungal communities detected on mango surfaces depended upon the studied factor. Results indicated that Burkholderiaceae (58.8%), Enterobacteriaceae (5.2%), Pseudomonadaceae (4.8%), Sphingomonadaceae (4.1%), Beijerinckiaceae (3.5%), and Microbacteriaceae (3.1%) were the dominant bacterial families across all samples. The majority of fungal sequences were assigned to Mycosphaerellaceae (34.5%), Cladosporiaceae (23.21%), Aureobasidiaceae (13.09%), Pleosporaceae (6.92%), Trichosphaeriaceae (5.17%), and Microstromatales_fam_Incertae_sedis (4.67%). For each studied location, mango fruit from each cultivar shared a core microbiome, and fruits of the same cultivar harvested in two different locations shared about 80% fungal and bacterial family taxa. The various factors tested in this study affected bacterial and fungal taxa differently, suggesting that some taxa could act as geographical (terroir) markers and in some cases as cultivar fingerprints. The ranking of the factors investigated in the present study showed that in decreasing order of importance: the plot (terroir), cultivar, fruit parts, harvest date and the position of the fruits are respectively the most impacting factors of the microbial flora, when compared to the orientation and the fruit position (height) on the tree. Overall, these findings provided insights on both bacterial and fungal diversity associated with the mango surface, their patterns from intra-fruit scale to local scale and the potential parameters shaping the mango microbiota.
A Systematic Review and Meta-Analysis of the Relationship Between Social Dominance Status and Common Behavioral Phenotypes in Male Laboratory Mice
Authors: Varholick Justin A. , Bailoo Jeremy D. , Jenkins Ashley , Voelkl Bernhard , Würbel Hanno
Published: January 2021

Background:Social dominance status (e.g., dominant or subordinate) is often associated with individual differences in behavior and physiology but is largely neglected in experimental designs and statistical analysis plans in biomedical animal research. In fact, the extent to which social dominance status affects common experimental outcomes is virtually unknown. Given the pervasive use of laboratory mice and culminating evidence of issues with reproducibility, understanding the role of social dominance status on common behavioral measures used in research may be of paramount importance.

Methods:To determine whether social dominance status—one facet of the social environment—contributes in a systematic way to standard measures of behavior in biomedical science, we conducted a systematic review of the existing literature searching the databases of PubMed, Embase, and Web of Science. Experiments were divided into several domains of behavior: exploration, anxiety, learned helplessness, cognition, social, and sensory behavior. Meta-analyses between experiments were conducted for the open field, elevated plus-maze, and Porsolt forced swim test.

Results:Of the 696 publications identified, a total of 55 experiments from 20 published studies met our pre-specified criteria. Study characteristics and reported results were highly heterogeneous across studies. A systematic review and meta-analyses, where possible, with these studies revealed little evidence for systematic phenotypic differences between dominant and subordinate male mice.

Conclusion:This finding contradicts the notion that social dominance status impacts behavior in significant ways, although the lack of an observed relationship may be attributable to study heterogeneity concerning strain, group-size, age, housing and husbandry conditions, and dominance assessment method. Therefore, further research considering these secondary sources of variation may be necessary to determine if social dominance generally impacts treatment effects in substantive ways.
Concurrency of Early-Age Exposure to Chinese Famine and Diabetes Increases Recurrence of Ischemic Stroke
Authors: Suo Yue , Chen Weiqi , Pan Yuesong , Li Hao , Meng Xia , Li Zixiao , Wang Chunjuan , Jing Jing , Wang Yilong , Wang Yongjun
Published: January 2021

Background and Purpose: Early age exposure to the Chinese Great Leap Forward famine (1959–1961) is associated with the incidence of risk factors for ischemic stroke. This study aims to examine the relationship between early age famine exposure and 12-month stroke recurrence. We sought to explore the interaction between famine exposure status and metabolic phenotypes on stroke recurrence and how the adherence of crucial evidence-based key performance indicators (KPI) would modify this interaction.

Methods: We analyzed data of patients who were born between 1953 and 1964 in the China National Stroke Registry II (CNSR-II). The study population was further divided into five subgroups for comparing 12-month stroke recurrence. A multivariate Cox proportional hazard regression model was used in analyzing the impact of the concurrence of metabolic phenotypes—type 2 diabetes (T2D) or metabolic syndrome (MetS)—and early-age famine exposure on recurrent risk. The influence of the adherence to predefined KPI and concurrency of metabolic phenotype was also evaluated.

Results: Concurrent T2D and early age famine exposure was associated with an increased recurrence risk of ischemic stroke with 12 months [adjusted hazard ratio (HR): 1.63, 95% confidence interval (CI) 1.28–2.07]. Optimal adherence to KPI was not associated with significantly reduced risk of 12-month stroke recurrence (adjusted HR: 0.80, 95% CI: 0.51–1.26).

Conclusions: Concurrency of early-age famine exposure and diabetes mellitus was associated with a higher risk of stroke recurrence within 12 months, and adherence to evidence-based KPI did not reduce the risk significantly.
The Correlation of a 2D Volume-Referencing Endolymphatic-Hydrops Grading System With Extra-Tympanic Electrocochleography in Patients With Definite Ménière's Disease
Authors: He Baihui , Zhang Fan , Zheng Hui , Sun Xiayu , Chen Junmin , Chen Jianyong , Liu Yupeng , Wang Lu , Wang Wei , Li Shuna , Yang Jun , Duan Maoli
Published: January 2021

Background: Although magnetic resonance imaging (MRI) of the membranous labyrinth and electrocochleography (ECochG) have been used to diagnose endolymphatic hydrops (ELH) in patients with Ménière's disease (MD), the relationship between imaging and ECochG is not well-documented.

Objectives: This study evaluates the ELH using 3D-FLAIR MRI and extra-tympanic ECochG (ET-ECochG) and correlates the results from 3D-FLAIR MRI to those from ET-ECochG.

Materials and Methods: 3D-FLAIR MRI images of 50 patients were assessed using a 2D volume-referencing grading system (VR scores, relative scores according to the known volumes of the cochlea, vestibule, and semicircular canals). Forty healthy subjects were included and compared to 51 definite MD ears of 50 patients while analyzing the ET-ECochG, which used a self-made bronze foil electrode. The amplitude ratio of the summating potential (SP) to the action potential (AP) (SP/AP) and the area ratio of SP to AP (Asp/Aap) were collected. Relative ELH grade scores were then correlated to ET-ECochG (SP/AP, Asp/Aap).

Results: The VR scores showed a better correlation (r = 0.88) with the pure tone average (PTA), disease duration, and vertigo frequency of MD than the Bernaerts scores (grading the cochlea and vestibule separately) (r = 0.22). The SP/AP and Asp/Aap of the unilateral MD patients were statistically comparable to those measured in contralateral ears and the results between the definite MD ears with healthy ears were statistically comparable (p < 0.05). In a ROC analysis Asp/Aap (area under curve, AUC 0.98) significantly (p = 0.01) outperformed SP/AP (AUC 0.91). The total score of ELH, vestibular ELH, and cochlear ELH were also correlated with SP/AP and Asp/Aap. The strongest correlation was found between the Asp/Aap and cochlear ELH (r = 0.60).

Conclusion: The 2D volume-referencing grading system was more meaningful than the Bernaerts scores. A correlation was found between ELH revealed by 3D-FLAIR MRI and the SP/AP of ET-ECochG in evaluating definite MD patients. The Asp/Aap appeared a more sensitive and reliable parameter than SP/AP for diagnosing the ELH of the membranous labyrinth.
Animal Models for Anorexia Nervosa—A Systematic Review
Authors: Scharner Sophie , Stengel Andreas
Published: January 2021

Anorexia nervosa is an eating disorder characterized by intense fear of gaining weight and a distorted body image which usually leads to low caloric intake and hyperactivity. The underlying mechanism and pathogenesis of anorexia nervosa is still poorly understood. In order to learn more about the underlying pathophysiology of anorexia nervosa and to find further possible treatment options, several animal models mimicking anorexia nervosa have been developed. The aim of this review is to systematically search different databases and provide an overview of existing animal models and to discuss the current knowledge gained from animal models of anorexia nervosa. For the systematic data search, the Pubmed—Medline database, Embase database, and Web of Science database were searched. After removal of duplicates and the systematic process of selection, 108 original research papers were included in this systematic review. One hundred and six studies were performed with rodents and 2 on monkeys. Eighteen different animal models for anorexia nervosa were used in these studies. Parameters assessed in many studies were body weight, food intake, physical activity, cessation of the estrous cycle in female animals, behavioral changes, metabolic and hormonal alterations. The most commonly used animal model (75 of the studies) is the activity-based anorexia model in which typically young rodents are exposed to time-reduced access to food (a certain number of hours a day) with unrestricted access to a running wheel. Of the genetic animal models, one that is of particular interest is the anx/anx mice model. Animal models have so far contributed many findings to the understanding of mechanisms of hunger and satiety, physical activity and cognition in an underweight state and other mechanisms relevant for anorexia nervosa in humans.
Chronic Intermittent Hypobaric Hypoxia Restores Hippocampus Function and Rescues Cognitive Impairments in Chronic Epileptic Rats via Wnt/β-catenin Signaling
Authors: Sun Can , Fu Jian , Qu Zhenzhen , Jia Lijing , Li Dongxiao , Zhen Junli , Wang Weiping
Published: January 2021

Epilepsy is a complex neurological disorder with frequent psychiatric, cognitive, and social comorbidities in addition to recurrent seizures. Cognitive impairment, one of the most common comorbidities, has severe adverse effects on quality of life. Chronic intermittent hypobaric hypoxia (CIHH) has demonstrated neuroprotective efficacy in several neurological disease models. In the present study, we examined the effects of CIHH on cognition and hippocampal function in chronic epileptic rats. CIHH treatment rescued deficits in spatial and object memory, hippocampal neurogenesis, and synaptic plasticity in pilocarpine-treated epileptic rats. The Wnt/β-catenin pathway has been implicated in neural stem cell proliferation and synapse development, and Wnt/β-catenin pathway inhibition effectively blocked the neurogenic effects of CIHH. Our findings indicate that CIHH rescues cognitive deficits in epileptic rats via Wnt/β-catenin pathway activation. This study establishes CIHH and Wnt/β-catenin pathway regulators as potential treatments for epilepsy- induced cognitive impairments.
Swallowing and Liking of Vegetable-Enriched Bread Compared With Commercial Breads as Evaluated by Older Adults
Authors: Amoah Isaac , Cairncross Carolyn , Rush Elaine
Published: January 2021

Characteristics of food that influence liking and ease-of-chewing and swallowing are not well-understood. Reformulation of bread to improve nutrient density may improve liking, ease-of-chewing and swallowing which could improve dietary intake particularly with aging. The study aimed to compare objectively and subjectively four breads of increasing nutrient density: $1 white (WB) and wheatmeal (WMB) commercial breads and two in-house formulations of vegetable-enriched breads (VB75 or VB100) which incorporated drum-dried pumpkin and sweet corn flours for physical, sensory and ease-of-chewing and swallowing properties. Each bread underwent instrumental texture analysis. The commercial and vegetable-enriched breads were not different by hardness or springiness but the vegetable breads were up to 25% less cohesive, less gummy and less chewy than the commercial breads. Questionnaires and Likert scale (150 mm) responses were completed by 50 physically active volunteers aged 50+ years. Overall liking of the VB75 and VB100 was rated 40% higher than the white and wheatmeal breads. Vegetable-enriched breads were rated as almost 50% easier to chew (mean ± SD; WB 70.53 ± 39.46 mm, WMB 77.68 ± 33.13 mm, VB75 104.78 ± 30.69 mm, VB100 107.58 ± 24.90 mm) and swallow (WB 70.29 ± 37.98 mm, WMB 77.53 ± 34.88 mm, VB75 104.63 ± 28.25 mm, VB100 104.90 ± 25.54 mm). Vegetable-enriched breads compared to white and wheatmeal breads were instrumentally and subjectively less gummy, cohesive and chewy than commercial breads and have the potential to both improve nutrition and “ease of swallowing” in older people. New areas of research should explore other underutilized vegetables for bread enrichment and their ability to aid swallowing and improve nutrition status.