bluuebunny/crossref_metadata_2025 huggingface dataset:
A Survey on Function Spaces of John–Nirenberg Type
Authors: Tao Jin , Yang Dachun , Yuan Wen
Published: September 2021

In this systematic review, the authors give a survey on the recent developments of both the John–Nirenberg space JNp and the space BMO as well as their vanishing subspaces such as VMO, XMO, CMO, VJNp, and CJNp on Rn or a given cube Q0⊂Rn with finite side length. In addition, some related open questions are also presented.
Relationships between Copper Futures Markets from the Perspective of Jump Diffusion
Authors: Jin Xue , Zhou Shiwei , Yin Kedong , Li Mingzhen
Published: September 2021

This paper analyzes the price correlation effect between domestic and foreign copper futures contracts. The VAR-BEKK-GARCH (1,1) spillover effect model and the BN-S class non-parametric model based on the jumping perspective are used. The co-integration test shows a long-term equilibrium relationship between the three copper futures markets, and the Granger causality test shows that copper futures contracts have significant two-way spillover effects between different periods in Shanghai for New York copper and unidirectional mean spillover effects for London copper. The BEKK model shows significant bidirectional fluctuation spillover effects between the futures contracts of the Shanghai, London, and New York copper markets before the stock market crash. After the crash, Shanghai and New York copper have significant one-way fluctuation spillover effects on London copper futures contracts. There are jumps within a single market, and the number of joint jumps between markets increases with the significance level.
Prognostic Factors in Lung Adenocarcinoma with Bone Metastasis Treated with EGFR-TKIs
Authors: Chiu Tzu-Hsuan , Lin Chun-Yu , Hsieh Meng-Heng , Lin Shu-Min , Fang Yueh-Fu
Published: September 2021

Background and Objectives: Patients who have advanced lung cancer and bone metastasis (BM) often suffer from skeletal-related events (SREs) that lead to poor quality of life and poor prognosis. Our study aimed to investigate the prognostic factors in patients with BM from epidermal growth factor receptor (EGFR) mutation-positive lung adenocarcinoma. Materials and Methods: This retrospective study included 77 lung adenocarcinoma patients with synchronous BM. These patients had first-line EGFR tyrosine kinase inhibitors (EGFR-TKIs) between January 2017 and December 2019. Among them, 42 patients were treated with 120 mg of subcutaneous denosumab monthly. We investigated their baseline characteristics, cancer management, SREs, progression-free survival (PFS), and overall survival (OS). Results: The PFS in the patients treated with or without denosumab were 10.1 vs. 12.5 months (p = 0.971). The median OS was 26.9 vs. 29.5 months (p = 0.967) in no denosumab and denosumab groups, respectively. Univariate analyses showed benefit of afatinib in PFS and good performance status in OS. Conclusion: Those patients that took afatinib as first-line EGFR-TKIs had significantly longer PFS than those treated with other TKIs. Denosumab had no prognostic effect on PFS or OS.
Langmuir Monolayer Techniques for the Investigation of Model Bacterial Membranes and Antibiotic Biodegradation Mechanisms
Authors: Rojewska Monika , Smułek Wojciech , Kaczorek Ewa , Prochaska Krystyna
Published: September 2021

The amounts of antibiotics of anthropogenic origin released and accumulated in the environment are known to have a negative impact on local communities of microorganisms, which leads to disturbances in the course of the biodegradation process and to growing antimicrobial resistance. This mini-review covers up-to-date information regarding problems related to the omnipresence of antibiotics and their consequences for the world of bacteria. In order to understand the interaction of antibiotics with bacterial membranes, it is necessary to explain their interaction mechanism at the molecular level. Such molecular-level interactions can be probed with Langmuir monolayers representing the cell membrane. This mini-review describes monolayer experiments undertaken to investigate the impact of selected antibiotics on components of biomembranes, with particular emphasis on the role and content of individual phospholipids and lipopolysaccharides (LPS). It is shown that the Langmuir technique may provide information about the interactions between antibiotics and lipids at the mixed film surface (π–A isotherm) and about the penetration of the active substances into the phospholipid monolayer model membranes (relaxation of the monolayer). Effects induced by antibiotics on the bacterial membrane may be correlated with their bactericidal activity, which may be vital for the selection of appropriate bacterial consortia that would ensure a high degradation efficiency of pharmaceuticals in the environment.
CO2/N2 Gas Separation Using Pebax/ZIF-7—PSf Composite Membranes
Authors: Yoon Soong-Seok , Lee Hyun-Kyung , Hong Se-Ryeong
Published: September 2021

In this study, we mixed the zeolitic imidazolate framework-7 (ZIF-7) with poly(ether-b-amide)® 2533 (Pebax-2533) and used it as a selective layer for a composite membrane. We prepared the composite membrane’s substrate using polysulfone (PSf), adjusted its pore size using polyethylene glycol (PEG), and applied polydimethylsiloxane (PDMS) to the gutter layer and the coating layer. Then, we investigated the membrane’s properties of gases by penetrating a single gas (N2, CO2) into the membrane. We identified the peaks and geometry of ZIF-7 to determine if it had been successfully synthesized. We confirmed that ZIF-7 had a BET surface area of 303 m2/g, a significantly high Langmuir surface area of 511 m2/g, and a high CO2/N2 adsorption selectivity of approximately 50. Considering the gas permeation, with ZIF-7 mixed into Pebax-2533, N2 permeation decreased from 2.68 GPU in a pure membrane to 0.43 GPU in the membrane with ZIF-7 25 wt%. CO2 permeation increased from 18.43 GPU in the pure membrane to 26.22 GPU in the ZIF-7 35 wt%. The CO2/N2 ideal selectivity increased from 6.88 in the pure membrane to 50.43 in the ZIF-7 25 wt%. Among the membranes, Pebax-2533/ZIF-7 25 wt% showed the highest permeation properties and the characteristics of CO2-friendly ZIF-7.
Mapping Bubble Formation and Coalescence in a Tubular Cross-Flow Membrane Foaming System
Authors: Deng Boxin , Neef Tessa , Schroën Karin , de Ruiter Jolet
Published: September 2021

Membrane foaming is a promising alternative to conventional foaming methods to produce uniform bubbles. In this study, we provide a fundamental study of a cross-flow membrane foaming (CFMF) system to understand and control bubble formation for various process conditions and fluid properties. Observations with high spatial and temporal resolution allowed us to study bubble formation and bubble coalescence processes simultaneously. Bubble formation time and the snap-off bubble size (D0) were primarily controlled by the continuous phase flow rate (Qc); they decreased as Qc increased, from 1.64 to 0.13 ms and from 125 to 49 µm. Coalescence resulted in an increase in bubble size (Dcoal>D0), which can be strongly reduced by increasing either continuous phase viscosity or protein concentration—factors that only slightly influence D0. Particularly, in a 2.5 wt % whey protein system, coalescence could be suppressed with a coefficient of variation below 20%. The stabilizing effect is ascribed to the convective transport of proteins and the intersection of timescales (i.e., μs to ms) of bubble formation and protein adsorption. Our study provides insights into the membrane foaming process at relevant (micro-) length and time scales and paves the way for its further development and application.
Enhanced Performance of Fly Ash-Based Supports for Low-Cost Ceramic Membranes with the Addition of Bauxite
Authors: Fan Wan , Zou Dong , Xu Jingrui , Chen Xianfu , Qiu Minghui , Fan Yiqun
Published: September 2021

Support is a necessary foundation for ceramic membranes to achieve high performance. Finding the optimum balance between high performance and low cost is still a significant challenge in the fabrication of ceramic supports. In this study, low-cost fly ash-based ceramic supports with enhanced performance were prepared by the addition of bauxite. The pore structure, mechanical strength, and shrinkage of fly ash/bauxite supports could be tuned by optimizing the bauxite content and sintering temperature. When the sintering temperature and bauxite content were controlled at 1300 °C and 40 wt%, respectively, the obtained membrane supports exhibited a high pure water permeance of approximately 5.36 m3·m−2·h−1·bar−1 and a high bending strength of approximately 69.6 MPa. At the same time, the optimized ceramic supports presented a typical mullite phase and excellent resistance to acid and alkali. This work provides a potential route for the preparation of ceramic membrane supports with characteristics of low cost and high performance.
Crack Growth Prediction on Critical Component for Structure Life Extension of Royal Malaysian Air Force (RMAF) Sukhoi Su-30MKM
Authors: Venugopal Arvinthan , Mohammad Roslina , Koslan Md Fuad Shah , Shafie Ashaari , Ali Alizarin bin , Eugene Owi
Published: September 2021

The critical aircraft structure, being the load-bearing members, is a vital component for any aircraft. The effect of fatigue loading, operating conditions, and environmental degradation has caused the structural integrity of the airframe to be assessed for its airworthiness requirement. Using the fatigue design concept of Safe Life, the RMAF adopts the Aircraft Structure Integrity Program (ASIP) to monitor the structural integrity of its critical components. RMAF has produced the task card using the engineering analysis concept on the aircraft’s critical structure. Various Computer-Aided Engineering (CAE) methods were used, and for this analysis, the Crack Growth Prediction method was used to determine the crack growth behavior and its ultimate failure point in case of any crack occurrences. Although there are six critical locations, the wing root is chosen since it has the highest possibility of fatigue failure. The analytical methods which were discussed are Crack Growth Analysis and Low Cycle Fatigue. For the numerical method, NX Nastran was used for the simulation of crack growth. The result from the crack growth analysis was validated with the numerical result. The conclusion is that, based on the fatigue life cycle, the wing root structure condition is not affected by severe damage, and its failure is approximately around 30 to 100 years for both the through hole and through side crack. Thus, its structural life can be extended. The research outcome will be on the extension of the structure life of the aircraft wing.
Analysis of the Dynamics of Rougher Cells on the Basis of Phenomenological Models and Discrete Event Simulation Framework
Authors: Saldaña Manuel , Neira Purísima , Flores Víctor , Moraga Carlos , Robles Pedro , Salazar Iván
Published: September 2021

Due to the increase in the amount of copper sulphide minerals processed through concentration processes and the need to improve the efficiency of these production processes, the development of theoretical models is making an important contribution to generating a better understanding of their dynamics, making it possible to identify the optimal conditions for the recovery of minerals, the impact of the independent variables in the responses, and the sensitivity of the recovery to variations in both the input variables and the operational parameters. This paper proposes a method for modeling, sensitizing, and optimizing the mineral recovery in rougher cells using a discrete event simulation (DES) framework and the fitting of analytical models on the basis of operational data from a concentration pilot plant. A sensitivity analysis was performed for low, medium, and high levels of the operative variables and/or parameters. The outcomes of the modeling indicate that the optimum mineral recovery is reached at medium levels of the flow rate of gas, bubble size, turbulence dissipation rate, surface tension, Reynolds number of bubble, bubble–particle contact angle, superficial gas velocity and gas hold-up in the froth zone. Additionally, the optimal response is reached at maximum levels of particle size and density and at minimum levels of bubble speed, fluid kinematic viscosity and fluid density in the sampled range. Finally, the recovery has an asymptotic behavior over time; however, the optimum recovery depends on an economic analysis, examining the marginalization of the response over time in an operational context.
Novel Experimentation for the Validation of Mechanistic Models to Describe Cold Dwell Sensitivity in Titanium Alloys
Authors: Sackett Elizabeth E. , Bache Martin R.
Published: September 2021

Previous mechanistic models, proposed to explain the process of damage accumulation and stress redistribution between strong and weak regions inherent within the microstructure of α/β and near α titanium alloys, are validated through a matrix of experiments employing a non-standard variant of the alloy Ti 685. The grain size of the model material was deliberately processed to offer grains up to 20 mm in diameter, to facilitate constitutive measurements within individual grains. A range of experiments were performed under static and cyclic loading, with the fatigue cycle conducted under either strain or load control. Data will be reported to demonstrate significant variations in elastic and plastic properties between grains and emphasise the role of time dependent strain accumulation. Implications for the “dwell sensitive fatigue” or “cold creep” response of conventional titanium alloys will be discussed.
Weld-Quality Prediction Algorithm Based on Multiple Models Using Process Signals in Resistance Spot Welding
Authors: Kim Sehyeon , Hwang Insung , Kim Dong-Yoon , Kim Young-Min , Kang Munjin , Yu Jiyoung
Published: September 2021

An efficient nondestructive testing method of resistance spot weld quality is essential in evaluating the weld quality of all welded joints in the automotive components of a car body production line. This study proposes a quality prediction algorithm for resistance spot welding that can predict the geometrical and physical properties of a spot-welded joint and evaluate weld quality based on quality acceptance criteria. To this end, four statistical models that predict the main geometrical and physical properties of a spot-welded joint, including tensile shear strength, indentation depth, expulsion occurrence, and failure mode, were estimated based on material information, dynamic resistance, and electrode displacement signals. The significance of the estimated models was then verified through an analysis of variance. The prediction accuracies of the models were 94.3%, 93.4%, 97.5%, and 85.0% for the tensile shear strength, indentation depth, expulsion occurrence, and failure modes, respectively. A weld quality evaluation methodology that can predict the properties of a spot-welded joint and evaluate the overall quality requirements based on authorized welding standards was proposed using the four statistical models.
Novel Ubiquitin Specific Protease-13 Inhibitors Alleviate Neurodegenerative Pathology
Authors: Liu Xiaoguang , Balaraman Kaluvu , Lynch Ciarán C. , Hebron Michaeline , Wolf Christian , Moussa Charbel
Published: September 2021

Ubiquitin Specific Protease-13 (USP13) promotes protein de-ubiquitination and is poorly understood in neurodegeneration. USP13 is upregulated in Alzheimer’s disease (AD) and Parkinson’s disease (PD), and USP13 knockdown via shRNA reduces neurotoxic proteins and increases proteasome activity in models of neurodegeneration. We synthesized novel analogues of spautin-1 which is a non-specific USP13 inhibitor but unable to penetrate the brain. Our synthesized small molecule compounds are able to enter the brain, more potently inhibit USP13, and significantly reduce alpha-synuclein levels in vivo and in vitro. USP13 inhibition in transgenic mutant alpha-synuclein (A53T) mice increased the ubiquitination of alpha-synuclein and reduced its protein levels. The data suggest that novel USP13 inhibitors improve neurodegenerative pathology via antagonism of de-ubiquitination, thus alleviating neurotoxic protein burden in neurodegenerative diseases.
Correlation between Pre-Ovulatory Follicle Diameter and Follicular Fluid Metabolome Profiles in Lactating Beef Cows
Authors: Read Casey C. , Edwards Lannett , Schrick Neal , Rhinehart Justin D. , Payton Rebecca R. , Campagna Shawn R. , Castro Hector F. , Klabnik Jessica L. , Horn Emma J. , Moorey Sarah E.
Published: September 2021

Induced ovulation of small pre-ovulatory follicles reduced pregnancy rates, embryo survival, day seven embryo quality, and successful embryo cleavage in beef cows undergoing fixed-time artificial insemination. RNA-sequencing of oocytes and associated cumulus cells collected from pre-ovulatory follicles 23 h after gonadotropin-releasing hormone (GnRH) administration to induce the pre-ovulatory gonadotropin surge suggested reduced capacity for glucose metabolism in cumulus cells of follicles ≤11.7 mm. We hypothesized that the follicular fluid metabolome influences metabolic capacity of the cumulus-oocyte complex and contributes to reduced embryo cleavage and quality grade observed following induced ovulation of small follicles. Therefore, we performed a study to determine the correlation between pre-ovulatory follicle diameter and follicular fluid metabolome profiles in lactating beef cows (Angus, n = 130). We synchronized the development of a pre-ovulatory follicle and collected the follicular contents approximately 20 h after GnRH administration. We then performed ultra-high performance liquid chromatography—high resolution mass spectrometry (UHPLC-HRMS) metabolomic studies on 43 follicular fluid samples and identified 38 metabolites within pre-ovulatory follicles of increasing size. We detected 18 metabolites with a significant, positive correlation to follicle diameter. Individual and pathway enrichment analysis of significantly correlated metabolites suggest that altered glucose and amino acid metabolism likely contribute to reduced developmental competence of oocytes when small pre-ovulatory follicles undergo induced ovulation.
Lipid Profile and Hepatic Fat Content Measured by 1H MR Spectroscopy in Patients before and after Liver Transplantation
Authors: Burian Martin , Hajek Milan , Sedivy Petr , Mikova Irena , Trunecka Pavel , Dezortova Monika
Published: September 2021

Increased hepatic fat content (HFC) is a hallmark of non-alcoholic fatty liver (NAFL) disease, a common condition in liver transplant recipients. Proton MR spectroscopy (1H MRS) and MR imaging-based proton density fat fraction as the only diagnosis modality enable precise non-invasive measurement of HFC and, also, fatty acid profiles in vivo. Using 1H MRS at 3T, we examined 47 liver transplantation candidates and 101 liver graft recipients. A point-resolved spectroscopy sequence was used to calculate the steatosis grade along with the saturated, unsaturated and polyunsaturated fractions of fatty acids in the liver. The steatosis grade measured by MRS was compared with the histological steatosis grade. HFC, represented by fat fraction values, is adept at distinguishing non-alcoholic steatohepatitis (NASH), NAFL and non-steatotic liver transplant patients. Relative hepatic lipid saturation increases while unsaturation decreases in response to increased HFC. Additionally, relative hepatic lipid saturation increases while unsaturation and polyunsaturation both decrease in liver recipients with histologically proven post-transplant NASH or NAFL compared to non-steatotic patients. HFC, measured by in vivo 1H MRS, correlated well with histological results. 1H MRS is a simple and fast method for in vivo analysis of HFC and its composition. It provides non-invasive support for NAFL and NASH diagnoses.
Metabolic Reprogramming of Mammary Epithelial Cells during TGF-β-Induced Epithelial-to-Mesenchymal Transition
Authors: Hua Wan , Kostidis Sarantos , Mayboroda Oleg , Giera Martin , Hornsveld Marten , ten Dijke Peter
Published: September 2021

The cytokine transforming growth factor-β (TGF-β) can induce normal breast epithelial cells to take on a mesenchymal phenotype, termed epithelial-to-mesenchymal transition (EMT). While the transcriptional and proteomic changes during TGF-β-induced EMT have been described, the metabolic rewiring that occurs in epithelial cells undergoing EMT is not well understood. Here, we quantitively analyzed the TGF-β-induced metabolic reprogramming during EMT of non-transformed NMuMG mouse mammary gland epithelial cells using nuclear magnetic resonance (NMR) spectroscopy. We found that TGF-β elevates glycolytic and tricarboxylic acid (TCA)-cycle activity and increases glutaminolysis. Additionally, TGF-β affects the hexosamine pathway, arginine-proline metabolism, the cellular redox state, and strongly affects choline metabolism during EMT. TGF-β was found to induce phosphocholine production. A kinase inhibitor RSM-93A that inhibits choline kinase α (CHKα) mitigated TGF-β-induced changes associated with EMT, i.e., increased filamentous (F)-actin stress fiber formation and N-Cadherin mesenchymal marker expression.
Investigation on Bidirectional Pulse Electrochemical Micromachining of Micro Dimples
Authors: Gu Zhouzhi , Chen Xiaolei , Xu Zhongzheng , Ye Zhisen , Li Guojun
Published: September 2021

Through-mask electrochemical micromachining (TMEMM) is a promising method to prepare micro dimples on the surface of metallic parts. However, the workpiece is machined one by one in traditional TMEMM. This paper introduced bidirectional pulse to TMEMM to improve the machining efficiency. Two masked workpieces were placed face to face, and connected to the ends of the bidirectional pulse power supply. Along with the change of the pulse direction, the polarities of the two workpieces were interchanged periodically, and micro dimples could be prepared on both workpieces at one time. The simulation and experiment results indicated that with bidirectional pulse mode, micro dimples with same the profile can be prepared on two workpieces at one time, and the dimension of micro dimple was smaller than that with unidirectional pulse mode. In bidirectional pulse current, the pulse frequency and pulse duty cycle played an important role on the preparation of micro dimple. With high pulse frequency and low pulse duty cycle, it is useful to reduce the undercut of micro dimple and improve the machining localization. With the pulse duty cycle of 20% and pulse frequency of 10 kHz, micro dimples with etch factor (EF) of 3 were well prepared on both workpieces surface.
Bioavailability of Organic Phosphorus Compounds to the Harmful Dinoflagellate Karenia mikimotoi
Authors: Huang Kaixuan , Zhuang Yanqing , Wang Zhou , Ou Linjian , Cen Jingyi , Lu Songhui , Qi Yuzao
Published: September 2021

Karenia mikimotoi is one of the most well-known harmful bloom species in temperate coastal waters. The present study investigated the characteristics of alkaline phosphatase (APase) and phosphodiesterase (PDEase) activities in hydrolysis of two phosphomonoesters (adenosine triphosphate (ATP) and ribulose 5-phosphate (R5P)) and a phosphodiester (cyclic adenosine monophosphate (cAMP)) in K. mikimotoi and compared its growth and physiological responses to the different forms of phosphorus substrates. K. mikimotoi produced comparable quantities of APase and PDEase to hydrolyze the organic phosphorus substrates, although hydrolysis of the phosphomonoesters was much faster than that of the phosphodiester. The growth of K. mikimotoi on organic phosphorus substrates was comparable to or better than that on inorganic phosphate. The difference in particulate organic nutrients (carbon, nitrogen, and phosphorus) and hemolytic activity supported different rates of hydrolysis-assimilation of the various organic phosphorus substrates by K. mikimotoi. The hemolytic activities of K. mikimotoi in the presence of organic phosphorus substrates were several times those in the presence of inorganic phosphate during the exponential phase. This suggested the potential important role of organic phosphorus in K. mikimotoi blooms.
Trends in Colouring Blue Glass in Central Europe in Relation to Changes in Chemical Composition of Glass from the Middle Ages to Modern Age
Authors: Zlámalová Cílová Zuzana , Gelnar Michal , Randáková Simona
Published: September 2021

The study deals with the development of the chemical composition of blue glass from the 13th to the 19th century in the region of Bohemia (Central Europe). Nearly 100 glass samples (colourless, greenish, and blue) were evaluated by an XRF method to distinguish the colouring components of blue glass. As early as in the 13th century, blue glass based on ash containing colouring ions of Co and Cu was produced here. To achieve the blue colour of glass, a copper-rich raw material was most likely applied. This information significantly complements the existing knowledge about glass colouring in the Middle Ages, as the glass of later periods was typically coloured with raw materials containing cobalt.
Metal Extraction and Recovery from Mobile Phone PCBs by a Combination of Bioleaching and Precipitation Processes
Authors: Santaolalla Arrate , Lens Piet N. L. , Barona Astrid , Rojo Naiara , Ocio Ainhoa , Gallastegui Gorka
Published: September 2021

Bearing in mind the metal rich composition of printed circuit boards (PCBs), this material represents a secondary source of valuable metals and offers an entrepreneurial opportunity in the metal sales market. Based on the ability of microorganisms to regenerate and produce the chemical oxidants that are responsible for metal leaching, bioleaching has become an efficient and affordable alternative to conventional metal recycling technologies, although further research is still necessary before industrial implementation. This study focuses on the recovery of metals contained in mobile phone PCBs through a combined process. Two different PCB pre-treatments were evaluated: grinding the whole piece and removing the epoxy cover from the piece without grinding. The benefit of A. ferrooxidans activity on the metal solubilization rate was analyzed. Additional chemical leaching assays were also conducted for comparison purposes and the reagents ferric iron (Fe3+) and sulfuric acid (H2SO4) were selected for these experiments. The copper extraction results obtained in Fe3+ experiments with and without bacteria (A. ferrooxidans) were similar after 260 h of operation, indicating the need for alternative strategies to ensure a controlled and continuous metal biodissolution rate. The contribution of H2SO4 to the leaching processes for copper and nickel was almost negligible during the first 50 h, and more significant thereafter. The recovered metals were precipitated from a synthetic solution simulating a real ferric leaching by adding sodium hydroxide (NaOH) and sodium sulfide (Na2S). The combination of both precipitants allowed an effective removal of metals from the leachate.
Correction: Witkowski et al. Estimation of Mining-Induced Horizontal Strain Tensor of Land Surface Applying InSAR. Minerals 2021, 11, 788
Authors: Witkowski Wojciech T. , Łukosz Magdalena , Guzy Artur , Hejmanowski Ryszard
Published: September 2021

The authors wish to make the following corrections to this paper [...]
A Rational Interpretation of the Role of Turbulence in Particle-Bubble Interactions
Authors: Yao Ning , Liu Jingting , Sun Xun , Liu Yan , Chen Songying , Wang Guichao
Published: September 2021

Interactions between particles and bubbles have been cornerstone for the successful applications of froth flotation to the beneficiations of minerals or coal. Particle-bubble interactions are highly physio-chemical processes on the basis of surface science and hydrodynamics. Though these two aspects are deeply interwoven, we focus on the discussions of the effects of turbulence on the interactions between particles and bubbles, i.e., collision, attachment and detachment. It has to be mentioned this effect is not working in one direction and can affect flotation performance in a complicated way. Only when turbulence effects are well understood, flotation processes can be optimised by suitably changing equipment structure or operating parameters. The aim of this paper is to review the most recent progresses in this aspect and to identify the future development in successfully considering turbulence effects on flotation processes.
Phase Transformation, Photocatalytic and Photoluminescent Properties of BiPO4 Catalysts Prepared by Solid-State Reaction: Degradation of Rhodamine B
Authors: Bouddouch Abdessalam , Amaterz Elhassan , Bakiz Bahcine , Taoufyq Aziz , Guinneton Frédéric , Villain Sylvie , Gavarri Jean-Raymond , Valmalette Jean-Christophe , Benlhachemi Abdeljalil
Published: September 2021

Polycrystalline bismuth phosphate BiPO4 was synthesized by solid-state reaction at different temperatures varying from 500 to 900 °C. The samples were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDS) and Raman spectroscopy. The low-temperature phase of BiPO4 has monoclinic structure with a space group P21/n, and was transformed into the monoclinic phase P21/m with a slight distortion of monoclinic lattice when it was heated above 500 °C. The effect of the transformation on the structure, morphology and photocatalytic properties was examined. The photocatalytic activity of each sample, in presence of Rhodamine B (RhB) in aqueous solution, was carried out and analyzed under UV light irradiation. Photoexperiments showed that the material prepared at 500 °C is the best catalyst with degradation efficiency of the order of 96% after 12 min of reaction time under UV light irradiation. This high photocatalytic efficiency could be due to their structural and morphological changes. The photocatalytic degradation mechanism of RhB in the presence of the best photocatalyst BiP-500 °C is proposed. The stability of the catalyst was also examined by carrying out four successive tests of the degradation in the presence of BiP-500 °C. Total organic carbon (TOC) was used to further estimate the rate of mineralization in the presence of BiP-500 °C (83% TOC removal). Photoluminescence experiments performed under UV-laser light irradiation revealed emissions in the green-orange range, with optimal intensities for the mix systems observed at 550 °C.
Xanthone Glucosides: Isolation, Bioactivity and Synthesis
Authors: Huang Qing , Wang Youyi , Wu Huaimo , Yuan Man , Zheng Changwu , Xu Hongxi
Published: September 2021

Xanthones are secondary metabolites found in plants, fungi, lichens, and bacteria from a variety of families and genera, with the majority found in the Gentianaceae, Polygalaceae, and Clusiaceae. They have a diverse range of bioactivities, including anti-oxidant, anti-bacterial, anti-malarial, anti-tuberculosis, and cytotoxic properties. Xanthone glucosides are a significant branch of xanthones. After glycosylation, xanthones may have improved characteristics (such as solubility and pharmacological activity). Currently, no critical review of xanthone glucosides has been published. A literature survey including reports of naturally occurring xanthone glucosides is included in this review. The isolation, structure, bioactivity, and synthesis of these compounds were all explored in depth.
Oxidative Stress and Multi-Organel Damage Induced by Two Novel Phytocannabinoids, CBDB and CBDP, in Breast Cancer Cells
Authors: Salbini Maria , Quarta Alessandra , Russo Fabiana , Giudetti Anna Maria , Citti Cinzia , Cannazza Giuseppe , Gigli Giuseppe , Vergara Daniele , Gaballo Antonio
Published: September 2021

Over the last few years, much attention has been paid to phytocannabinoids derived from Cannabis for their therapeutic potential. Δ9-tetrahydrocannabinol (Δ9-THC) and cannabidiol (CBD) are the most abundant compounds of the Cannabis sativa L. plant. Recently, novel phytocannabinoids, such as cannabidibutol (CBDB) and cannabidiphorol (CBDP), have been discovered. These new molecules exhibit the same terpenophenolic core of CBD and differ only for the length of the alkyl side chain. Roles of CBD homologs in physiological and pathological processes are emerging but the exact molecular mechanisms remain to be fully elucidated. Here, we investigated the biological effects of the newly discovered CBDB or CBDP, compared to the well-known natural and synthetic CBD (nat CBD and syn CBD) in human breast carcinoma cells that express CB receptors. In detail, our data demonstrated that the treatment of cells with the novel phytocannabinoids affects cell viability, increases the production of reactive oxygen species (ROS) and activates cellular pathways related to ROS signaling, as already demonstrated for natural CBD. Moreover, we observed that the biological activity is significantly increased upon combining CBD homologs with drugs that inhibit the activity of enzymes involved in the metabolism of endocannabinoids, such as the monoacylglycerol lipase (MAGL) inhibitor, or with drugs that induces the activation of cellular stress pathways, such as the phorbol ester 12-myristate 13-acetate (PMA).
A Review on Current Status of Biochar Uses in Agriculture
Authors: Allohverdi Tara , Mohanty Amar Kumar , Roy Poritosh , Misra Manjusri
Published: September 2021

In a time when climate change increases desertification and drought globally, novel and effective solutions are required in order to continue food production for the world’s increasing population. Synthetic fertilizers have been long used to improve the productivity of agricultural soils, part of which leaches into the environment and emits greenhouse gasses (GHG). Some fundamental challenges within agricultural practices include the improvement of water retention and microbiota in soils, as well as boosting the efficiency of fertilizers. Biochar is a nutrient rich material produced from biomass, gaining attention for soil amendment purposes, improving crop yields as well as for carbon sequestration. This study summarizes the potential benefits of biochar applications, placing emphasis on its application in the agricultural sector. It seems biochar used for soil amendment improves nutrient density of soils, water holding capacity, reduces fertilizer requirements, enhances soil microbiota, and increases crop yields. Additionally, biochar usage has many environmental benefits, economic benefits, and a potential role to play in carbon credit systems. Biochar (also known as biocarbon) may hold the answer to these fundamental requirements.
Cold Atmospheric Plasma Jet as a Possible Adjuvant Therapy for Periodontal Disease
Authors: Lima Gabriela de Morais Gouvêa , Borges Aline Chiodi , Nishime Thalita Mayumi Castaldelli , Santana-Melo Gabriela de Fatima , Kostov Konstantin Georgiev , Mayer Marcia Pinto Alves , Koga-Ito Cristiane Yumi
Published: September 2021

Due to the limitations of traditional periodontal therapies, and reported cold atmospheric plasma anti-inflammatory/antimicrobial activities, plasma could be an adjuvant therapy to periodontitis. Porphyromonas gingivalis was grown in blood agar. Standardized suspensions were plated on blood agar and plasma-treated for planktonic growth. For biofilm, dual-species Streptococcus gordonii + P. gingivalis biofilm grew for 48 h and then was plasma-treated. XTT assay and CFU counting were performed. Cytotoxicity was accessed immediately or after 24 h. Plasma was applied for 1, 3, 5 or 7 min. In vivo: Thirty C57BI/6 mice were subject to experimental periodontitis for 11 days. Immediately after ligature removal, animals were plasma-treated for 5 min once—Group P1 (n = 10); twice (Day 11 and 13)—Group P2 (n = 10); or not treated—Group S (n = 10). Mice were euthanized on day 15. Histological and microtomography analyses were performed. Significance level was 5%. Halo diameter increased proportionally to time of exposure contrary to CFU/mL counting. Mean/SD of fibroblasts viability did not vary among the groups. Plasma was able to inhibit P. gingivalis in planktonic culture and biofilm in a cell-safe manner. Moreover, plasma treatment in vivo, for 5 min, tends to improve periodontal tissue recovery, proportionally to the number of plasma applications.
Curcumin and Nano-Curcumin Mitigate Copper Neurotoxicity by Modulating Oxidative Stress, Inflammation, and Akt/GSK-3β Signaling
Authors: Sarawi Wedad S. , Alhusaini Ahlam M. , Fadda Laila M. , Alomar Hatun A. , Albaker Awatif B. , Aljrboa Amjad S. , Alotaibi Areej M. , Hasan Iman H. , Mahmoud Ayman M.
Published: September 2021

Copper (Cu) is essential for multiple biochemical processes, and copper sulphate (CuSO4) is a pesticide used for repelling pests. Accidental or intentional intoxication can induce multiorgan toxicity and could be fatal. Curcumin (CUR) is a potent antioxidant, but its poor systemic bioavailability is the main drawback in its therapeutic uses. This study investigated the protective effect of CUR and N-CUR on CuSO4-induced cerebral oxidative stress, inflammation, and apoptosis in rats, pointing to the possible involvement of Akt/GSK-3β. Rats received 100 mg/kg CuSO4 and were concurrently treated with CUR or N-CUR for 7 days. Cu-administered rats exhibited a remarkable increase in cerebral malondialdehyde (MDA), NF-κB p65, TNF-α, and IL-6 associated with decreased GSH, SOD, and catalase. Cu provoked DNA fragmentation, upregulated BAX, caspase-3, and p53, and decreased BCL-2 in the brain of rats. N-CUR and CUR ameliorated MDA, NF-κB p65, and pro-inflammatory cytokines, downregulated pro-apoptotic genes, upregulated BCL-2, and enhanced antioxidants and DNA integrity. In addition, both N-CUR and CUR increased AKT Ser473 and GSK-3β Ser9 phosphorylation in the brain of Cu-administered rats. In conclusion, N-CUR and CUR prevent Cu neurotoxicity by attenuating oxidative injury, inflammatory response, and apoptosis and upregulating AKT/GSK-3β signaling. The neuroprotective effect of N-CUR was more potent than CUR.
Synthesis of Tailored Perfluoro Unsaturated Monomers for Potential Applications in Proton Exchange Membrane Preparation
Authors: Monopoli Antonio , Casiello Michele , Cotugno Pietro , Milella Antonella , Palumbo Fabio , Fracassi Francesco , Nacci Angelo
Published: September 2021

The aim of the present work is the synthesis and characterization of new perfluorinated monomers bearing, similarly to Nafion®, acidic groups for proton transport for potential and future applications in proton exchange membrane (PEM) fuel cells. To this end, we focused our attention on the synthesis of various molecules with (i) sufficient volatility to be used in vacuum polymerization techniques (e.g., PECVD)), (ii) sulfonic, phosphonic, or carboxylic acid functionalities for proton transport capacity of the resulting membrane, (iii) both aliphatic and aromatic perfluorinated tags to diversify the membrane polarity with respect to Nafion®, and (iv) a double bond to facilitate the polymerization under vacuum giving a preferential way for the chain growth of the polymer. A retrosynthetic approach persuaded us to attempt three main synthetic strategies: (a) organometallic Heck-type cross-coupling, (b) nucleophilic displacement, and (c) Wittig–Horner reaction (carbanion approach). Preliminary results on the plasma deposition of a polymeric film are also presented. The variation of plasma conditions allowed us to point out that the film prepared in the mildest settings (20 W) shows the maximum monomer retention in its structure. In this condition, plasma polymerization likely occurs mainly by rupture of the π bond in the monomer molecule.
Directed Evolution Methods for Enzyme Engineering
Authors: Nirantar Saurabh Rajendra
Published: September 2021

Enzymes underpin the processes required for most biotransformations. However, natural enzymes are often not optimal for biotechnological uses and must be engineered for improved activity, specificity and stability. A rich and growing variety of wet-lab methods have been developed by researchers over decades to accomplish this goal. In this review such methods and their specific attributes are examined.
Touch Imprint Intraoperative Flow Cytometry as a Complementary Tool for Detailed Assessment of Resection Margins and Tumor Biology in Liver Surgery for Primary and Metastatic Liver Neoplasms
Authors: Markopoulos Georgios , Glantzounis Georgios , Goussia Anna , Lianos Georgios , Karampa Anastasia , Alexiou George , Vartholomatos George
Published: September 2021

Liver resection is the main treatment for primary and metastatic liver tumors in order to achieve long-term survival with good quality of life. The ultimate goal of surgical oncology is to achieve complete tumor removal with adequate clear surgical margins. Flow cytometry is a powerful analytical technique with applications such as phenotypic analysis and quantification of DNA content. Intraoperative flow cytometry (iFC) is the application of flow cytometry for DNA content/ploidy and cell cycle distribution analysis during surgery for tumor cell analysis and margin evaluation. It has been used for cell analysis of intracranial tumors and recently of head and neck carcinomas and breast carcinomas, as well as for tumor margin evaluation. Herein, we present a novel touch imprint iFC protocol for the detailed assessment of tumor margins during excision of malignant hepatic lesions. The protocol aims to offer information on surgical margins after removal of malignant liver tumors based on DNA content of cancer cells and to corroborate the results of iFC with that of histopathological analysis. Based on the established role of iFC in other types of malignancies, our specialized protocol has the potential, through characterization of cells in liver transection surface post hepatectomy, to offer significant information on the type of resection and tumor biology. This information can be used to effectively guide intra- and postoperative patient management.
Microdialysis on Ex Vivo Porcine Ear Skin Can Validly Study Dermal Penetration including the Fraction of Transfollicular Penetration—Demonstrated on Caffeine Nanocrystals
Authors: Klein Anna Lena , Lubda Markus , Specht David , Pyo Sung-Min , Busch Loris , Lademann Jürgen , Meinke Martina C. , Beckers Ingeborg , von Hagen Jörg , Keck Cornelia M. , Patzelt Alexa
Published: September 2021

Common ex vivo methods for penetration investigations often fail to monitor transfollicular penetration appropriately. In the present investigation, the validity of dermal microdialysis on the ex vivo porcine ear skin to investigate penetration kinetics, including transfollicular penetration, was studied. In setup A, a caffeine nanocrystal formulation was compared to a non-particular caffeine gel formulation. In setup B, two caffeine nanocrystal formulations of different sizes (200 nm, 700 nm) were compared to each other. Microdialysis samples were collected for 46 h. After sampling, the skin layers were separated, homogenized, and caffeine was quantified in all samples. In setup A the area under the curve (AUC) after crystal gel formulation application was 12 times higher than after non-particular formulation application. Setup B showed an increased AUC of 42% in the microdialysis data when the 700 nm caffeine crystals were applied compared to the 200 nm crystals. The microdialysis data was supported by the separation, homogenization and extraction data. Microdialysis performed on ex vivo porcine ear skin is a novel experimental setup. It is of high interest for further investigations since it is able to also capture the impact of follicular and transfollicular penetration kinetics as no other ex vivo setup can.
X-ray Fluorescence Spectroscopy Features of Micro- and Nanoscale Copper and Nickel Particle Compositions
Authors: Chebakova Kristina A. , Dzidziguri Ella L. , Sidorova Elena N. , Vasiliev Andrey A. , Ozherelkov Dmitriy Yu. , Pelevin Ivan A. , Gromov Alexander A. , Nalivaiko Anton Yu.
Published: September 2021

The study is devoted to X-ray fluorescence spectroscopy (XRF) features of micro- and nanosized powder mixtures of copper and nickel. XRF is a high accuracy method that allows for both qualitative and quantitative analysis. However, the XRF measurement error due to the size of the studied particles is not usually taken into account, which limits the use of the method in some cases, such as analysis of Ni-Cu mixtures and coatings. In this paper, a method for obtaining copper and nickel nanoparticles was investigated, and the XRF of powder compositions was considered in detail. The initial micro- and nanoparticles of copper and nickel were studied in detail using SEM, TEM, XRD, and EDX. Based on experimental data, calibration curves for copper-nickel powder compositions of various sizes were developed. According to the results, it was experimentally established that the calibration curves constructed for nanoscale and microscale powders differ significantly. The presented approach can be expanded for other metals and particle sizes.
Precise Control of Green to Blue Emission of Halide Perovskite Nanocrystals Using Terbium Chloride as Chlorine Source
Authors: Deng Wenqiang , Fan Ting , Lü Jiantao , Li Jingling , Deng Tingting , Liu Mingqi
Published: September 2021

CsPbClxBr3-x nanocrystals were prepared by ligand-assisted deposition at room temperature, and their wavelength was accurately adjusted by doping TbCl3. The synthesized nanocrystals were monoclinic and the morphology was almost unchanged after doping. The fluorescence emission of CsPbClxBr3-x nanocrystals was easily controlled from green to blue by adjusting the amount of TbCl3, which realizes the continuous and accurate spectral regulation in the range of green to blue. This method provides a new scheme for fast anion exchange of all-inorganic perovskite nanocrystals in an open environment at room temperature.
The Effect of Interface Diffusion on Raman Spectra of Wurtzite Short-Period GaN/AlN Superlattices
Authors: Davydov Valery , Roginskii Evgenii M. , Kitaev Yuri , Smirnov Alexander , Eliseyev Ilya , Zavarin Eugene , Lundin Wsevolod , Nechaev Dmitrii , Jmerik Valentin , Smirnov Mikhail , Pristovsek Markus , Shubina Tatiana
Published: September 2021

We present an extensive theoretical and experimental study to identify the effect on the Raman spectrum due to interface interdiffusion between GaN and AlN layers in short-period GaN/AlN superlattices (SLs). The Raman spectra for SLs with sharp interfaces and with different degree of interface diffusion are simulated by ab initio calculations and within the framework of the random-element isodisplacement model. The comparison of the results of theoretical calculations and experimental data obtained on PA MBE and MOVPE grown SLs, showed that the bands related to A1(LO) confined phonons are very sensitive to the degree of interface diffusion. As a result, a correlation between the Raman spectra in the range of A1(LO) confined phonons and the interface quality in SLs is obtained. This opens up new possibilities for the analysis of the structural characteristics of short-period GaN/AlN SLs using Raman spectroscopy.