bluuebunny/crossref_metadata_2025 huggingface dataset:
Incomplete Ileocecal Bypass for Ileal Pathology in Horses: 21 Cases (2012–2019)
Authors: Giusto Gessica , Cerullo Anna , Labate Federico , Gandini Marco
Published: February 2021

Background: Incomplete ileocecal bypass can be performed in cases in which an ileal disfunction is suspected but resection of the diseased ileum is not necessary. Objectives: To describe the clinical findings, the surgical technique, and the outcome of 21 cases of colic with ileal pathologies that underwent an incomplete ileocecal bypass. Methods: Historical, clinical, and surgical features of cases diagnosed with pathologies involving the ileum or the ileocecal valve that underwent ileocecal anastomosis without ileal resection were retrieved. Clinical (heart rate, duration of symptoms, presence of reflux, age, weight at arrival) and surgical (surgical pathology, duration of surgery, type of anastomosis) data were retrieved and analysed. Data on short term survival and postoperative complications (colic, post-operative reflux, incisional infection, fever), length of hospital stay, and long term follow up were also obtained. Results: A total of 21 horses met the criteria; 13 horses had ileal impaction (one with muscular hypertrophy), 5 horses had epiploic foramen entrapment, and 3 horses had a pedunculated lipoma. An incomplete ileocecal bypass was performed with a two-layer hand-sewn side-to-side technique in 19 cases and with a stapled side-to-side technique in 2 cases. Short term survival was 95.2%. At 12-months follow up, all horses but two were alive, and 13 of the 14 sport horses returned to their previous level of activity. Long term survival was 90.47%. Conclusions Incomplete ileocecal bypass may represent a valid surgical technique in case of ileocecal valve disfunction when ileum resection is not necessary; this technique may represent an alternative to extensive manipulation without subsequent recurrence of ileal impaction.
Grape Stem Extracts with Potential Anticancer and Antioxidant Properties
Authors: Quero Javier , Jiménez-Moreno Nerea , Esparza Irene , Osada Jesús , Cerrada Elena , Ancín-Azpilicueta Carmen , Rodríguez-Yoldi María Jesús
Published: February 2021

The application of plant extracts for therapeutic purposes has been used in traditional medicine because plants contain bioactive compounds with beneficial properties for health. Currently, the use of these compounds that are rich in polyphenols for the treatment and prevention of diseases such as cancer, diabetes, and cardiovascular diseases, many of them related to oxidative stress, is gaining certain relevance. Polyphenols have been shown to have antimutagenic, antioxidant, and anti-inflammatory properties. Therefore, the objective of the present work was to study the potential effect of grape stem extracts (GSE), rich in phenolic compounds, in the treatment of cancer, as well as their role in the prevention of this disease associated with its antioxidant power. For that purpose, three cancer lines (Caco-2, MCF-7, and MDA-MB-231) were used, and the results showed that grape stem extracts were capable of showing an antiproliferative effect in these cells through apoptosis cell death associated with a modification of the mitochondrial potential and reactive oxygen species (ROS) levels. Additionally, grape stem extracts showed an antioxidant effect on differentiated intestinal cells that could protect the intestine from diseases related to oxidative stress. Therefore, grape extracts contain bioactive principles with important biological properties and could be used as bio-functional food ingredients to prevent diseases or even to improve certain aspects of human health.
Speech Intelligibility Analysis and Approximation to Room Parameters through the Internet of Things
Authors: Lopez-Ballester Jesus , Alcaraz Calero Jose M. , Segura-Garcia Jaume , Felici-Castell Santiago , Garcia-Pineda Miguel , Cobos Maximo
Published: February 2021

In recent years, Wireless Acoustic Sensor Networks (WASN) have been widely applied to different acoustic fields in outdoor and indoor environments. Most of these applications are oriented to locate or identify sources and measure specific features of the environment involved. In this paper, we study the application of a WASN for room acoustic measurements. To evaluate the acoustic characteristics, a set of Raspberry Pi 3 (RPi) has been used. One is used to play different acoustic signals and four are used to record at different points in the room simultaneously. The signals are sent wirelessly to a computer connected to a server, where using MATLAB we calculate both the impulse response (IR), and different acoustic parameters, such as the Speech Intelligibility Index (SII). In this way, the evaluation of room acoustic parameters with asynchronous IR measurements two different applications has been explored. Finally, the network features have been evaluated to assess the effectiveness of this system.
Constrained Backtracking Matching Pursuit Algorithm for Image Reconstruction in Compressed Sensing
Authors: Bi Xue , Leng Lu , Kim Cheonshik , Liu Xinwen , Du Yajun , Liu Feng
Published: February 2021

Image reconstruction based on sparse constraints is an important research topic in compressed sensing. Sparsity adaptive matching pursuit (SAMP) is a greedy pursuit reconstruction algorithm, which reconstructs signals without prior information of the sparsity level and potentially presents better reconstruction performance than other greedy pursuit algorithms. However, SAMP still suffers from being sensitive to the step size selection at high sub-sampling ratios. To solve this problem, this paper proposes a constrained backtracking matching pursuit (CBMP) algorithm for image reconstruction. The composite strategy, including two kinds of constraints, effectively controls the increment of the estimated sparsity level at different stages and accurately estimates the true support set of images. Based on the relationship analysis between the signal and measurement, an energy criterion is also proposed as a constraint. At the same time, the four-to-one rule is improved as an extra constraint. Comprehensive experimental results demonstrate that the proposed CBMP yields better performance and further stability than other greedy pursuit algorithms for image reconstruction.
Curricular Analytics to Characterize Educational Trajectories in High-Failure Rate Courses That Lead to Late Dropout
Authors: Salazar-Fernandez Juan Pablo , Sepúlveda Marcos , Munoz-Gama Jorge , Nussbaum Miguel
Published: February 2021

Late dropout is one of the most pressing challenges currently facing higher education, and the process that each student follows to arrive at that decision usually involves several academic periods. This work presents a curricular analytics approach at the program level, to analyze how educational trajectories of undergraduate students in high-failure rate courses help to describe the process that leads to late dropout. Educational trajectories (n = 10,969) of high-failure rate courses are created using Process Mining techniques, and the results are discussed based on established theoretical frameworks. Late dropout was more frequent among students who took a stopout while having high-failure rate courses they must retake. Furthermore, students who ended in late dropout with high-failure rate courses they must retake had educational trajectories that were on average shorter and less satisfactory. On the other hand, the educational trajectories of students who ended in late dropout without high-failure rate courses they must retake were more similar to those of students who graduated late. Moreover, some differences found among ISCED fields are also described. The proposed approach can be replicated in any other university to understand the educational trajectories of late dropout students from a longitudinal perspective, generating new knowledge about the dynamic behavior of the students. This knowledge can trigger improvements to the curriculum and in the follow-up mechanisms used to increase student retention.
GPU-Enabled Serverless Workflows for Efficient Multimedia Processing
Authors: Risco Sebastián , Moltó Germán
Published: February 2021

Serverless computing has introduced scalable event-driven processing in Cloud infrastructures. However, it is not trivial for multimedia processing to benefit from the elastic capabilities featured by serverless applications. To this aim, this paper introduces the evolution of a framework to support the execution of customized runtime environments in AWS Lambda in order to accommodate workloads that do not satisfy its strict computational requirements: increased execution times and the ability to use GPU-based resources. This has been achieved through the integration of AWS Batch, a managed service to deploy virtual elastic clusters for the execution of containerized jobs. In addition, a Functions Definition Language (FDL) is introduced for the description of data-driven workflows of functions. These workflows can simultaneously leverage both AWS Lambda for the highly-scalable execution of short jobs and AWS Batch, for the execution of compute-intensive jobs that can profit from GPU-based computing. To assess the developed open-source framework, we executed a case study for efficient serverless video processing. The workflow automatically generates subtitles based on the audio and applies GPU-based object recognition to the video frames, thus simultaneously harnessing different computing services. This allows for the creation of cost-effective highly-parallel scale-to-zero serverless workflows in AWS.
Numerical Study of Engine Performance and Emissions for Port Injection of Ammonia into a Gasoline\Ethanol Dual-Fuel Spark Ignition Engine
Authors: Salek Farhad , Babaie Meisam , Shakeri Amin , Hosseini Seyed Vahid , Bodisco Timothy , Zare Ali
Published: February 2021

This study aims to investigate the effect of the port injection of ammonia on performance, knock and NOx emission across a range of engine speeds in a gasoline/ethanol dual-fuel engine. An experimentally validated numerical model of a naturally aspirated spark-ignition (SI) engine was developed in AVL BOOST for the purpose of this investigation. The vibe two zone combustion model, which is widely used for the mathematical modeling of spark-ignition engines is employed for the numerical analysis of the combustion process. A significant reduction of ~50% in NOx emissions was observed across the engine speed range. However, the port injection of ammonia imposed some negative impacts on engine equivalent BSFC, CO and HC emissions, increasing these parameters by 3%, 30% and 21%, respectively, at the 10% ammonia injection ratio. Additionally, the minimum octane number of primary fuel required to prevent knock was reduced by up to 3.6% by adding ammonia between 5 and 10%. All in all, the injection of ammonia inside a bio-fueled engine could make it robust and produce less NOx, while having some undesirable effects on BSFC, CO and HC emissions.
4D Building Reconstruction with Machine Learning and Historical Maps
Authors: Farella Elisa Mariarosaria , Özdemir Emre , Remondino Fabio
Published: February 2021

The increasing importance of three-dimensional (3D) city modelling is linked to these data’s different applications and advantages in many domains. Images and Light Detection and Ranging (LiDAR) data availability are now an evident and unavoidable prerequisite, not always verified for past scenarios. Indeed, historical maps are often the only source of information when dealing with historical scenarios or multi-temporal (4D) digital representations. The paper presents a methodology to derive 4D building models in the level of detail 1 (LoD1), inferring missing height information through machine learning techniques. The aim is to realise 4D LoD1 buildings for geospatial analyses and visualisation, valorising historical data, and urban studies. Several machine learning regression techniques are analysed and employed for deriving missing height data from digitised multi-temporal maps. The implemented method relies on geometric, neighbours, and categorical attributes for height prediction. Derived elevation data are then used for 4D building reconstructions, offering multi-temporal versions of the considered urban scenarios. Various evaluation metrics are also presented for tackling the common issue of lack of ground-truth information within historical data.
Research Progress on Synergistic Technologies of Agricultural Multi-Robots
Authors: Mao Wenju , Liu Zhijie , Liu Heng , Yang Fuzeng , Wang Meirong
Published: February 2021

Multi-robots have shown good application prospects in agricultural production. Studying the synergistic technologies of agricultural multi-robots can not only improve the efficiency of the overall robot system and meet the needs of precision farming but also solve the problems of decreasing effective labor supply and increasing labor costs in agriculture. Therefore, starting from the point of view of an agricultural multiple robot system architectures, this paper reviews the representative research results of five synergistic technologies of agricultural multi-robots in recent years, namely, environment perception, task allocation, path planning, formation control, and communication, and summarizes the technological progress and development characteristics of these five technologies. Finally, because of these development characteristics, it is shown that the trends and research focus for agricultural multi-robots are to optimize the existing technologies and apply them to a variety of agricultural multi-robots, such as building a hybrid architecture of multi-robot systems, SLAM (simultaneous localization and mapping), cooperation learning of robots, hybrid path planning and formation reconstruction. While synergistic technologies of agricultural multi-robots are extremely challenging in production, in combination with previous research results for real agricultural multi-robots and social development demand, we conclude that it is realistic to expect automated multi-robot systems in the future.
UHPLC-MS Method for the Analysis of the Molecular Adjuvant Sulfavant A
Authors: Nuzzo Genoveffa , Manzo Emiliano , Ziaco Marcello , Fioretto Laura , Campos Ana Margarida , Gallo Carmela , d’Ippolito Giuliana , Fontana Angelo
Published: February 2021

A fast and sensitive method that is based on Ultra High Performance Liquid Chromatography coupled with High Resolution Mass Spectrometry (UHPLC-HRMS) for the measurement of Sulfavant A, a molecular adjuvant with a sulfolipid skeleton, is described. The method has been validated over the linearity range of 2.5–2000 ngmL−1 using a deuterated derivative (d70-Sulfavant A) as internal standard. Chromatographic separation is based on a UHPLC Kinetex® 2.6 µm PS C18 column and a gradient of methanol in 0.32 mM ammonium hydroxide solution buffered at pH 8. The lowest limit of quantification of Sulfavant A was 6.5 ngmL−1. The analytical procedure was tested on an extract of mice lung spiked with 30, 300, and 1500 ng of Sulfavant A. The analysis revealed a precision and accuracy value (as a mean value of all the quality control samples analyzed) of 4.7% and 96% in MeOH and 6.4% and 93.4% in the lung extracts, respectively.
An Assessment of ERA5 Reanalysis for Antarctic Near-Surface Air Temperature
Authors: Zhu Jiangping , Xie Aihong , Qin Xiang , Wang Yetang , Xu Bing , Wang Yicheng
Published: February 2021

The European Center for Medium-Range Weather Forecasts (ECMWF) released its latest reanalysis dataset named ERA5 in 2017. To assess the performance of ERA5 in Antarctica, we compare the near-surface temperature data from ERA5 and ERA-Interim with the measured data from 41 weather stations. ERA5 has a strong linear relationship with monthly observations, and the statistical significant correlation coefficients (p < 0.05) are higher than 0.95 at all stations selected. The performance of ERA5 shows regional differences, and the correlations are high in West Antarctica and low in East Antarctica. Compared with ERA5, ERA-Interim has a slightly higher linear relationship with observations in the Antarctic Peninsula. ERA5 agrees well with the temperature observations in austral spring, with significant correlation coefficients higher than 0.90 and bias lower than 0.70 °C. The temperature trend from ERA5 is consistent with that from observations, in which a cooling trend dominates East Antarctica and West Antarctica, while a warming trend exists in the Antarctic Peninsula except during austral summer. Generally, ERA5 can effectively represent the temperature changes in Antarctica and its three subregions. Although ERA5 has bias, ERA5 can play an important role as a powerful tool to explore the climate change in Antarctica with sparse in situ observations.
Role of Pirin, an Oxidative Stress Sensor Protein, in Epithelial Carcinogenesis
Authors: Perez-Dominguez Francisco , Carrillo-Beltrán Diego , Blanco Rancés , Muñoz Juan P. , León-Cruz Grettell , Corvalan Alejandro H. , Urzúa Ulises , Calaf Gloria M. , Aguayo Francisco
Published: February 2021

Pirin is an oxidative stress (OS) sensor belonging to the functionally diverse cupin superfamily of proteins. Pirin is a suggested quercetinase and transcriptional activator of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway. Its biological role in cancer development remains a novel area of study. This review presents accumulating evidence on the contribution of Pirin in epithelial cancers, involved signaling pathways, and as a suggested therapeutic target. Finally, we propose a model in which Pirin is upregulated by physical, chemical or biological factors involved in OS and cancer development.
Alleviation of Copper-Induced Stress in Pea (Pisum sativum L.) through Foliar Application of Gibberellic Acid
Authors: Javed Talha , Ali Muhammad Moaaz , Shabbir Rubab , Anwar Raheel , Afzal Irfan , Mauro Rosario Paolo
Published: February 2021

Copper (Cu) is an essential metal for plants. However, its excess in soil can adversely affect plant metabolism. The current study evaluated the effects of gibberellic acid (GA3) foliar applications on the performance of pea plants grown either in Cu-contaminated (Cu+) and non-contaminated (Cu−) soil. GA3 was sprayed (0, 10, 50, and 100 mg·L−1) on 15-days-old plants. The results showed that the increasing concentration of GA3 buffered the phytotoxic effects of Cu and enhanced plant growth, photosynthesis, and leaf chlorophyll content. Foliar-sprayed GA3 up to 100 mg·L−1 alleviated the oxidative stress, as inferred from the lower concentrations of MDA and H2O2 (33.3 µmol·g−1 and 182 µmol·g−1, respectively), and boosted the activity of superoxide dismutase (64.4 U·g−1·FW), peroxidase (122.7 U·g−1·FW), and catalase (226.3 U·g−1·FW). Interestingly, GA3 promoted Cu accumulation in different plant parts when compared to untreated plants, likely due to increased photosynthetic and transpiration rates. Overall, foliar application of GA3 promoted phytoextraction of Cu and alleviated the oxidative stress in pea plants grown in Cu+ soil.
Relationship between Muscle Mass and Non-Alcoholic Fatty Liver Disease
Authors: Lee Jun-Hyuk , Lee Hye-Sun , Lee Byoung-Kwon , Kwon Yu-Jin , Lee Ji-Won
Published: February 2021

Although sarcopenia is known to be a risk factor for non-alcoholic fatty liver disease (NAFLD), whether NAFLD is a risk factor for the development of sarcopenia is not clear. We investigated relationships between NAFLD and low skeletal muscle mass index (LSMI) using three different datasets. Participants were classified into LSMI and normal groups. LSMI was defined as a body mass index (BMI)-adjusted appendicular skeletal muscle mass <0.789 in men and <0.512 in women or as the sex-specific lowest quintile of BMI-adjusted total skeletal muscle mass. NAFLD was determined according to NAFLD liver fat score or abdominal ultrasonography. The NAFLD groups showed a higher hazard ratios (HRs) with 95% confidence intervals (CIs) for LSMI than the normal groups (HRs = 1.21, 95% CIs = 1.05–1.40). The LSMI groups also showed a higher HRs with 95% CIs for NAFLD than normal groups (HRs = 1.56, 95% CIs = 1.38–1.78). Participants with NAFLD had consistently less skeletal muscle mass over 12 years of follow-up. In conclusion, LSMI and NAFLD showed a relationship. Maintaining muscle mass should be emphasized in the management of NAFLD.
A Multifocal Pediatric Papillary Thyroid Carcinoma (PTC) Harboring the AGK-BRAF and RET/PTC3 Fusion in a Mutually Exclusive Pattern Reveals Distinct Levels of Genomic Instability and Nuclear Organization
Authors: Sisdelli Luiza , Cordioli Maria Isabel V. , Vaisman Fernanda , Monte Osmar , Longui Carlos A. , Cury Adriano N. , Freitas Monique O. , Rangel-Pozzo Aline , Mai Sabine , Cerutti Janete M.
Published: February 2021

The spectrum and incidence of gene fusions in papillary thyroid carcinoma (PTC) can differ significantly depending on the age of onset, histological subtype or radiation exposure history. In sporadic pediatric PTC, RET/PTC1-3 and AGK-BRAF fusions are common genetic alterations. The role of RET/PTC as a prognostic marker in pediatric PTC is still under investigation. We recently showed that AGK-BRAF fusion is prevalent in young patients (mean 10 years) and associated with specific and aggressive pathological features such as multifocality and lung metastasis. In this pilot study, we report a unique patient harboring three different foci: the first was positive for AGK-BRAF fusion, the second was positive for just RET/PTC3 fusion and the third was negative for both rearrangements. To investigate whether AGK-BRAF and RET/PTC3 are associated with genomic instability and chromatin modifications, we performed quantitative fluorescence in situ hybridization (Q-FISH) of telomere repeats followed by 3D imaging analysis and 3D super-resolution Structured Illumination Microscopy (3D-SIM) to analyze the DNA structure from the foci. We demonstrated in this preliminary study that AGK-BRAF is likely associated with higher levels of telomere-related genomic instability and chromatin remodeling in comparison with RET/PTC3 foci. Our results suggest a progressive disruption in chromatin structure in AGK-BRAF-positive cells, which might explain a more aggressive disease outcome in patients harboring this rearrangement.
Comprehensive Analysis and Expression Profiling of PIN, AUX/LAX, and ABCB Auxin Transporter Gene Families in Solanum tuberosum under Phytohormone Stimuli and Abiotic Stresses
Authors: Yang Chenghui , Wang Dongdong , Zhang Chao , Ye Minghui , Kong Nana , Ma Haoli , Chen Qin
Published: February 2021

Auxin is the only plant hormone that exhibits transport polarity mediated by three families: auxin resistant (AUX) 1/like AUX1 (LAX) influx carriers, pin-formed (PIN) efflux carriers, and ATP-binding cassette B (ABCB) influx/efflux carriers. Extensive studies about the biological functions of auxin transporter genes have been reported in model plants. Information regarding these genes in potato remains scarce. Here, we conducted a comprehensive analysis of auxin transporter gene families in potato to examine genomic distributions, phylogeny, co-expression analysis, gene structure and subcellular localization, and expression profiling using bioinformatics tools and qRT-PCR analysis. From these analyses, 5 StLAXs, 10 StPINs, and 22 StABCBs were identified in the potato genome and distributed in 10 of 18 gene modules correlating to the development of various tissues. Transient expression experiments indicated that three representative auxin transporters showed plasma membrane localizations. The responsiveness to auxin and auxin transport inhibitors implied their possible roles in mediating intercellular auxin homoeostasis and redistribution. The differential expression under abscisic acid and abiotic stresses indicated their specific adaptive mechanisms regulating tolerance to environmental stimuli. A large number of auxin-responsive and stress-related cis-elements within their promoters could account for their responsiveness to diverse stresses. Our study aimed to understand the biological significance of potato auxin transporters in hormone signaling and tolerance to environmental stresses.
The Emerging Function of PKCtheta in Cancer
Authors: Nicolle Amandine , Zhang Ye , Belguise Karine
Published: February 2021

Protein Kinase C theta (PKCθ) is a serine/threonine kinase that belongs to the novel PKC subfamily. In normal tissue, its expression is restricted to skeletal muscle cells, platelets and T lymphocytes in which PKCθ controls several essential cellular processes such as survival, proliferation and differentiation. Particularly, PKCθ has been extensively studied for its role in the immune system where its translocation to the immunological synapse plays a critical role in T cell activation. Beyond its physiological role in immune responses, increasing evidence implicates PKCθ in the pathology of various diseases, especially autoimmune disorders and cancers. In this review, we discuss the implication of PKCθ in various types of cancers and the PKCθ-mediated signaling events controlling cancer initiation and progression. In these types of cancers, the high PKCθ expression leads to aberrant cell proliferation, migration and invasion resulting in malignant phenotype. The recent development and application of PKCθ inhibitors in the context of autoimmune diseases could benefit the emergence of treatment for cancers in which PKCθ has been implicated.
Spermine and Spermidine Priming against Botrytis cinerea Modulates ROS Dynamics and Metabolism in Arabidopsis
Authors: Janse van Rensburg Henry Christopher , Limami Anis M. , Van den Ende Wim
Published: February 2021

Polyamines (PAs) are ubiquitous small aliphatic polycations important for growth, development, and environmental stress responses in plants. Here, we demonstrate that exogenous application of spermine (Spm) and spermidine (Spd) induced cell death at high concentrations, but primed resistance against the necrotrophic fungus Botrytis cinerea in Arabidopsis. At low concentrations, Spm was more effective than Spd. Treatments with higher exogenous Spd and Spm concentrations resulted in a biphasic endogenous PA accumulation. Exogenous Spm induced the accumulation of H2O2 after treatment but also after infection with B. cinerea. Both Spm and Spd induced the activities of catalase, ascorbate peroxidase, and guaiacol peroxidase after treatment but also after infection with B. cinerea. The soluble sugars glucose, fructose, and sucrose accumulated after treatment with high concentrations of PAs, whereas only Spm induced sugar accumulation after infection. Total and active nitrate reductase (NR) activities were inhibited by Spm treatment, whereas Spd inhibited active NR at low concentrations but promoted active NR at high concentrations. Finally, γaminobutyric acid accumulated after treatment and infection in plants treated with high concentrations of Spm. Phenylalanine and asparagine also accumulated after infection in plants treated with a high concentration of Spm. Our data illustrate that Spm and Spd are effective in priming resistance against B. cinerea, opening the door for the development of sustainable alternatives for chemical pesticides.
Genomic Context and Mechanisms of the ACVR1 Mutation in Fibrodysplasia Ossificans Progressiva
Authors: Ravazzolo Roberto , Bocciardi Renata
Published: February 2021

Basic research in Fibrodysplasia Ossificans Progressiva (FOP) was carried out in the various fields involved in the disease pathophysiology and was important for designing therapeutic approaches, some of which were already developed as ongoing or planned clinical trials. Genetic research was fundamental in identifying the FOP causative mutation, and the astonishing progress in technologies for genomic analysis, coupled to related computational methods, now make possible further research in this field. We present here a review of molecular and cellular factors which could explain why a single mutation, the R206H in the ACVR1 gene, is absolutely prevalent in FOP patients. We also address the mechanisms by which FOP expressivity could be modulated by cis-acting variants in the ACVR1 genomic region in human chromosome 2q. Finally, we also discuss the general issue of genetic modifiers in FOP.
Attention-Dependent Physiological Correlates in Sleep-Deprived Young Healthy Humans
Authors: Cesari Valentina , Marinari Elena , Laurino Marco , Gemignani Angelo , Menicucci Danilo
Published: February 2021

Cognitive functions could be specifically altered but masked from the unspecific effect of workload, a common factor affecting cognitive functions that modulate peripheral outputs. To identify workload-related and specific, task-dependent components, physiological correlates of cognitive functioning were derived by studying 15 healthy volunteers performing attentional tasks in baseline and post-sleep-deprivation conditions (one week interval). Sleep deprivation was introduced to increase workload. We performed recordings of heart pulse, facial temperature, and head movements during tasks assessing attentional network efficiency (ANT, Attentional Network Task; CCT, Continuous Compensatory Tracker) workload assessments after execution of tasks. Changes in cognitive and physiological indices were studied in both conditions; physiological correlates of cognitive performance were identified by correlating changes from baseline to post-sleep-deprivation condition of task indices with those of physiological measures after correction for between-conditions workload changes. We found that mental and physical demands of workload increased after sleep deprivation. We identified no changes in cognitive and physiological indices across conditions; specific physiological correlates of attentional systems, as indicated by the negative correlation between changes in ANT-alerting and changes in amplitude of head movements and the positive correlation between changes in CCT-speed indexing alertness and changes in facial temperature.
Emerging Trends in Neoadjuvant Chemotherapy for Ovarian Cancer
Authors: Patel Ami , Iyer Puja , Matsuzaki Shinya , Matsuo Koji , Sood Anil K. , Fleming Nicole D.
Published: February 2021

Epithelial ovarian cancer remains a leading cause of death amongst all gynecologic cancers despite advances in surgical and medical therapy. Historically, patients with ovarian cancer underwent primary tumor reductive surgery followed by postoperative chemotherapy; however, neoadjuvant chemotherapy followed by interval tumor reductive surgery has gradually become an alternative approach for patients with advanced-stage ovarian cancer for whom primary tumor reductive surgery is not feasible. Decision-making about the use of these approaches has not been uniform. Hence, it is essential to identify patients who can benefit most from neoadjuvant chemotherapy followed by interval tumor reductive surgery. Several prospective and retrospective studies have proposed potential models to guide upfront decision-making for patients with advanced ovarian cancer. In this review, we summarize important decision-making models that can improve patient selection for personalized treatment. Models based on clinical factors (clinical parameters, radiology studies and laparoscopy scoring) and molecular markers (circulating and tumor-based) are useful, but laparoscopic staging is among the most informative diagnostic methods for upfront decision-making in patients medically fit for surgery. Further research is needed to explore more reliable models to determine personalized treatment for advanced epithelial ovarian cancer.
Grading Evolution and Contemporary Prognostic Biomarkers of Clinically Significant Prostate Cancer
Authors: Sopyllo Konrad , Erickson Andrew M. , Mirtti Tuomas
Published: February 2021

Gleason grading remains the strongest prognostic parameter in localized prostate adenocarcinoma. We have here outlined the evolution and contemporary practices in pathological evaluation of prostate tissue samples for Gleason score and Grade group. The state of more observer-independent grading methods with the aid of artificial intelligence is also reviewed. Additionally, we conducted a systematic review of biomarkers that hold promise in adding independent prognostic or predictive value on top of clinical parameters, Grade group and PSA. We especially focused on hard end points during the follow-up, i.e., occurrence of metastasis, disease-specific mortality and overall mortality. In peripheral blood, biopsy-detected prostate cancer or in surgical specimens, we can conclude that there are more than sixty biomarkers that have been shown to have independent prognostic significance when adjusted to conventional risk assessment or grouping. Our search brought up some known putative markers and panels, as expected. Also, the synthesis in the systematic review indicated markers that ought to be further studied as part of prospective trials and in well characterized patient cohorts in order to increase the resolution of the current clinico-pathological prognostic factors.
Large-Scale Proteomic Analysis of Follicular Lymphoma Reveals Extensive Remodeling of Cell Adhesion Pathway and Identifies Hub Proteins Related to the Lymphomagenesis
Authors: Duś-Szachniewicz Kamila , Rymkiewicz Grzegorz , Agrawal Anil Kumar , Kołodziej Paweł , Wiśniewski Jacek R.
Published: February 2021

Follicular lymphoma (FL) represents the major subtype of indolent B-cell non-Hodgkin lymphomas (B-NHLs) and results from the malignant transformation of mature B-cells in lymphoid organs. Although gene expression and genomic studies have identified multiple disease driving gene aberrations, only a few proteomic studies focused on the protein level. The present work aimed to examine the proteomic profiles of follicular lymphoma vs. normal B-cells obtained by fine-needle aspiration biopsy (FNAB) to gain deep insight into the most perturbed pathway of FL. The cells of interest were purified by magnetic-activated cell sorting (MACS). High-throughput proteomic profiling was performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and allowed to identify of 6724 proteins in at least 75% of each group of samples. The ‘Total Protein Approach’ (TPA) was applied to the absolute quantification of proteins in this study. We identified 1186 differentially abundant proteins (DAPs) between FL and control samples, causing an extensive remodeling of several molecular pathways, including the B-cell receptor signaling pathway, cellular adhesion molecules, and PPAR pathway. Additionally, the construction of protein–protein interactions networks (PPINs) and identification of hub proteins allowed us to indicate the key player proteins for FL pathology. Finally, ICAM1, CD9, and CD79B protein expression was validated in an independent cohort by flow cytometry (FCM), and the results were consistent with the mass spectrometry (MS) data.
Patient-Reported Outcomes (PROs) and Health-Related Quality of Life (HR-QoL) in Patients with Ovarian Cancer: What Is Different Compared to Healthy Women?
Authors: Inci Melisa Guelhan , Richter Rolf , Heise Kathrin , Dukatz Ricarda , Woopen Hannah , Sehouli Jalid
Published: February 2021

Introduction: The aim of this analysis was to evaluate the health-related quality of life (HR-QoL) in patients with ovarian cancer using a patient-reported outcome (PRO) based questionnaire and to compare it to the healthy female population in Germany and to other ovarian cancer patients worldwide. Additionally, we looked for differences in the HR-QoL with respect to the patients’ ages in our cohort. Methods: The HR-QoL for 155 enrolled patients with ovarian cancer was assessed by the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire (EORTC QLQ-C30) prior to surgery and then compared with 501 healthy females in Germany, as well as to the previously published European Organization for Research and Treatment of Cancer (EORTC) reference data for 917 patients with ovarian cancer worldwide. Moreover, we grouped our cohort by ages <65 and >65 years and analyzed them for further differences. To identify the differences, T-tests were applied. Results: Overall, 155 patients were enrolled, and 126 patients had advanced-stage ovarian cancer (FIGO III–IV) (82.4%). Fifty-five (36%) patients were >65 years. Except for the physical functioning scale, all other domains of the functioning scales were significantly lower in our patients with ovarian cancer than in the healthy female population. The emotional (50 points versus 60 points, p = 0.02), cognitive (76 points versus 88 points, p = 0.005), and social functioning scales (68 points versus 81 points, p = 0.006) were lower in the younger subgroup. Further, the younger subgroup exhibited significantly more fatigue (40 points versus 29 points, p = 0.03) and financial difficulties (20 points versus 2 points, p < 0.001) than the older subgroup. Discussion: Interestingly, the patients with ovarian cancer had no significant differences in the physical functioning scale when compared with the healthy women. In contrast, the patients, especially in the younger group, needed special support for the emotional and social areas of their daily lives.
Low Temperature Water-Gas Shift: Enhancing Stability through Optimizing Rb Loading on Pt/ZrO2
Authors: Watson Caleb Daniel , Martinelli Michela , Cronauer Donald Charles , Kropf A. Jeremy , Jacobs Gary
Published: February 2021

Recent studies have shown that appropriate levels of alkali promotion can significantly improve the rate of low-temperature water gas shift (LT-WGS) on a range of catalysts. At sufficient loadings, the alkali metal can weaken the formate C–H bond and promote formate dehydrogenation, which is the proposed rate determining step in the formate associative mechanism. In a continuation of these studies, the effect of Rb promotion on Pt/ZrO2 is examined herein. Pt/ZrO2 catalysts were prepared with several different Rb loadings and characterized using temperature programmed reduction mass spectrometry (TPR-MS), temperature programmed desorption (TPD), diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS), an X-ray absorption near edge spectroscopy (XANES) difference procedure, extended X-ray absorption fine structure spectroscopy (EXAFS) fitting, TPR-EXAFS/XANES, and reactor testing. At loadings of 2.79% Rb or higher, a significant shift was seen in the formate ν(CH) band. The results showed that a Rb loading of 4.65%, significantly improves the rate of formate decomposition in the presence of steam via weakening the formate C–H bond. However, excessive rubidium loading led to the increase in stability of a second intermediate, carbonate and inhibited hydrogen transfer reactions on Pt through surface blocking and accelerated agglomeration during catalyst activation. Optimal catalytic performance was achieved with loadings in the range of 0.55–0.93% Rb, where the catalyst maintained high activity and exhibited higher stability in comparison with the unpromoted catalyst.
Influence of Photo-Deposited Pt and Pd onto Chromium Doped TiO2 Nanotubes in Photo-Electrochemical Water Splitting for Hydrogen Generation
Authors: Sharifi Tayebeh , Mohammadi Tecush , Momeni Mohamad Mohsen , Kusic Hrvoje , Kraljic Rokovic Marijana , Loncaric Bozic Ana , Ghayeb Yousef
Published: February 2021

Hydrogen (H2) is considered as an ideal fuel for the future. The photo-electrochemical (PEC) water splitting employing semiconducting materials and induced irradiation, preferably of solar spectrum, presents a viable route for H2 production. In this work, self-ordered chromium-doped TiO2 nanotube (CT) was fabricated using in-situ electro-anodization. CT surface modification was then performed by photo-deposition of Pt and Pd particles, producing Pt-CT and Pd-CT catalysts, respectively. Their morphological features, crystallinity, surface composition, and optical absorption have been inspected by scanning electron microscopy (SEM), X-ray diffraction (XRD), energy dispersive X-ray spectroscopy (EDX), Raman, and UV–vis absorption spectroscopy. Linear sweep voltammetry, chronoamperometry, and open circuit potential methods have been applied to study PEC activities of Pt-CT and Pd-CT catalysts in a form of electrodes. It was found that Pt-CT and Pd-CT electrodes possess excellent photo-generated electron/hole (e−/h+) separation and transport properties. The enhanced photocurrent responses of 4 and 3 times more than that of CT are revealed for Pt-CT and Pd-CT, respectively. The activity of as-prepared Pt-CT and Pd-CT catalysts was then tested for H2 generation. The maximum amount of the evolved H2 followed decreasing order: 1.08 > 0.65 > 0.26 mL cm−2 h−1 for Pt-CT, Pd-CT, and CT electrodes, respectively, clearly showing the positive contribution of photo-deposited (nano)particles onto CT surface.
Targeting Myeloid-Derived Suppressor Cells in Ovarian Cancer
Authors: Mabuchi Seiji , Sasano Tomoyuki , Komura Naoko
Published: February 2021

Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population of immature myeloid cells that exhibit immunosuppressive activity. They also directly stimulate tumor cell proliferation, metastasis, and angiogenesis. In ovarian cancer, there are increased numbers of circulating or tumor-infiltrating MDSCs, and increased frequencies of MDSCs are associated with a poor prognosis or an advanced clinical stage. Moreover, in murine models of ovarian cancer, MDSC depletion has shown significant growth-inhibitory effects and enhanced the therapeutic efficacy of existing anticancer therapies. In this review, we summarize the current knowledge on MDSC biology, clinical significance of MDSC, and potential MDSC-targeting strategies in ovarian cancer.
Directed Differentiation of Human Pluripotent Stem Cells towards Corneal Endothelial-Like Cells under Defined Conditions
Authors: Grönroos Pyry , Ilmarinen Tanja , Skottman Heli
Published: February 2021

The most crucial function of corneal endothelial cells (CEnCs) is to maintain optical transparency by transporting excess fluid out of stroma. Unfortunately, CEnCs are not able to proliferate in vivo in the case of trauma or dystrophy. Visually impaired patients with corneal endothelial deficiencies that are waiting for transplantation due to massive global shortage of cadaveric corneal transplants are in a great need of help. In this study, our goal was to develop a defined, clinically applicable protocol for direct differentiation of CEnCs from human pluripotent stem cells (hPSCs). To produce feeder-free hPSC-CEnCs, we used small molecule induction with transforming growth factor (TGF) beta receptor inhibitor SB431542, GSK-3-specific inhibitor CHIR99021 and retinoic acid to guide differentiation through the neural crest and periocular mesenchyme (POM). Cells were characterized by the morphology and expression of human (h)CEnC markers with immunocytochemistry and RT-qPCR. After one week of induction, we observed the upregulation of POM markers paired-like homeodomain transcription factor 2 (PITX2) and Forkhead box C1 (FOXC1) and polygonal-shaped cells expressing CEnC-associated markers Zona Occludens-1 (ZO-1), sodium-potassium (Na+/K+)-ATPase, CD166, sodium bicarbonate cotransporter 1 (SLC4A4), aquaporin 1 (AQP1) and N-cadherin (NCAD). Furthermore, we showed that retinoic acid induced a dome formation in the cell culture, with a possible indication of fluid transport by the differentiated cells. Thus, we successfully generated CEnC-like cells from hPSCs with a defined, simple and fast differentiation method.
The Role of HSPB8, a Component of the Chaperone-Assisted Selective Autophagy Machinery, in Cancer
Authors: Cristofani Riccardo , Piccolella Margherita , Crippa Valeria , Tedesco Barbara , Montagnani Marelli Marina , Poletti Angelo , Moretti Roberta M.
Published: February 2021

The cellular response to cancer-induced stress is one of the major aspects regulating cancer development and progression. The Heat Shock Protein B8 (HSPB8) is a small chaperone involved in chaperone-assisted selective autophagy (CASA). CASA promotes the selective degradation of proteins to counteract cell stress such as tumor-induced stress. HSPB8 is also involved in (i) the cell division machinery regulating chromosome segregation and cell cycle arrest in the G0/G1 phase and (ii) inflammation regulating dendritic cell maturation and cytokine production. HSPB8 expression and role are tumor-specific, showing a dual and opposite role. Interestingly, HSPB8 may be involved in the acquisition of chemoresistance to drugs. Despite the fact the mechanisms of HSPB8-mediated CASA activation in tumors need further studies, HSPB8 could represent an important factor in cancer induction and progression and it may be a potential target for anticancer treatment in specific types of cancer. In this review, we will discuss the molecular mechanism underlying HSPB8 roles in normal and cancer conditions. The basic mechanisms involved in anti- and pro-tumoral activities of HSPB8 are deeply discussed together with the pathways that modulate HSPB8 expression, in order to outline molecules with a beneficial effect for cancer cell growth, migration, and death.
Least-Squares Finite Element Method for a Meso-Scale Model of the Spread of COVID-19
Authors: Bertrand Fleurianne , Pirch Emilie
Published: February 2021

This paper investigates numerical properties of a flux-based finite element method for the discretization of a SEIQRD (susceptible-exposed-infected-quarantined-recovered-deceased) model for the spread of COVID-19. The model is largely based on the SEIRD (susceptible-exposed-infected-recovered-deceased) models developed in recent works, with additional extension by a quarantined compartment of the living population and the resulting first-order system of coupled PDEs is solved by a Least-Squares meso-scale method. We incorporate several data on political measures for the containment of the spread gathered during the course of the year 2020 and develop an indicator that influences the predictions calculated by the method. The numerical experiments conducted show a promising accuracy of predictions of the space-time behavior of the virus compared to the real disease spreading data.
Coronary Flow and Reserve by Enhanced Transthoracic Doppler Trumps Coronary Anatomy by Computed Tomography in Assessing Coronary Artery Stenosis
Authors: Caiati Carlo , Scardapane Arnaldo , Iacovelli Fortunato , Pollice Paolo , Achille Teresa Immacolata , Favale Stefano , Lepera Mario Erminio
Published: February 2021

We report the case of a 71-year-old patient with many risk factors for coronary atherosclerosis, who underwent computed coronary angiography (CTA), in accordance with the guidelines, for recent onset atypical chest pain. CTA revealed critical (>50% lumen diameter narrowing) stenosis of the proximal anterior descending coronary, and the patient was scheduled for invasive coronary angiography (ICA). Before ICA he underwent enhanced transthoracic echo-Doppler (E-Doppler TTE) for coronary flow detection by color-guided pulsed-wave Doppler recording of the left main (LMCA) and whole left anterior descending coronary artery (LAD,) along with coronary flow reserve (CFR) in the distal LAD calculated as the ratio, of peak flow velocity during i.v. adenosine (140 mcg/Kg/m) to resting flow velocity. E-Doppler TTE mapping revealed only mild stenosis (28% area narrowing) of the mid LAD and a CFR of 3.20, in perfect agreement with the color mapping showing no flow limiting stenosis in the LMCA and LAD. ICA revealed only a very mild stenosis in the mid LAD and mild atherosclerosis in the other coronaries (intimal irregularities). Thus, coronary stenosis was better predicted by E-Doppler TTE than by CTA. Coronary flow and reserve as assessed by E-Doppler TTE trumps coronary anatomy as assessed by CTA, without exposing the patient to harmful radiation and iodinated contrast medium.