bluuebunny/crossref_metadata_2025 huggingface dataset:
Regional Planning in a Decentralised State: How Administrative Practices contributed to Consensus-Building in Sixteenth-Century Holland
Authors: van Tielhof Milja
Published: July 2017

This article examines how a regional drainage system in the northern part of Holland in the Late Middle Ages could emerge despite the fact that the weak central state was hardly able to provide the necessary coordination nor prevent free-riding. Institutions, defined as rules and norms, including practices, procedures and techniques, play a key role in the argument. Four traditional administrative practices are identified as essential to the emergence of regional water control: a broad consultation process, by which opponents of new plans were also heard; landowners giving their explicit consent to plans and their costs; the proportional division of the costs; and the use of compensation for damage suffered. These practices respected local autonomy and broadened the level of support among the local stakeholders. The effectiveness of the practices was strengthened when they were used in combination with a technique provided by the Habsburg state: the enqueste or inquiry. This was a technique for gathering reliable, relevant and detailed information at the local and regional level. In this case study, the information on landscape and water use, collected by the 1544 Commission of Inquiry, facilitated cooperation between communities that enjoyed a high degree of self-governance.
Forecasting Failure Rates of Electronic Goods by Using Decomposition and Fuzzy Clustering of Empirical Failure Rate Curves
Authors: Jónás Tamás , Árva Gábor , Tóth Zsuzsanna Eszter
Published: July 2017

In this paper a novel methodology founded on the joint application of analytic decomposition of empirical failure rate time series and soft computational techniques is introduced in order to predict bathtub-shaped failure rate curves of consumer electronic goods. Empirical failure rate time series are modeled by a flexible function the parameters of which have geometric interpretations, and so the model parameters grab the characteristics of bathtub-shaped failure rate curves. The so-called typical standardized failure rate curve models, which are derived from the model functions through standardization and fuzzy clustering processes, are applied to predict failure rate curves of consumer electronics in a method that combines analytic curve fitting and soft computing techniques. The forecasting capability of the introduced method was tested on real-life data. Based on the empirical results from practical applications, the introduced method can be considered as a new, alternative reliability prediction technique the application of which can support the electronic repair service providers to plan their resources in the long run.
A Diagnosis of Some Dynamical Processes Underlying a Higher-Latitude Typhoon Using the Multiscale Window Transform
Authors: Wang Lan , Liang X. San
Published: July 2017

The typhoon Damrey that hit Jiangsu the populous coastal province of China on 2 August 2012 is a rarely seen tropical cyclone in that it has a higher latitude origin, and it is part of a twin typhoon. In this study, a recently developed analysis tool—multiscale window transform (MWT) and the MWT-based localized multiscale energy and vorticity analysis (MS-EVA)—is applied to investigate its genesis and maintenance. The fields are reconstructed onto three scale windows: large-scale, tropical cyclone–scale, and cumulus convection–scale windows. It is found that the track of Damrey is well along the edge of the subtropical high. Its birth is mainly caused by a strong barotropic instability in the lower troposphere around 24°–25° N. Its later amplification, however, is quite different, related to a baroclinic instability in the upper troposphere. Also discovered in this study is that a strong center of upscale canonical transfer exists over the East China Sea at the mouth of Yangtze River, which accounts for the rapid decay of Damrey before landing.
Air Pollution Monitoring in the south-east baltic using the epiphytic lichen hypogymnia physodes
Authors: Koroleva Yulia , Revunkov Vladimir
Published: July 2017

Abstract: Epiphytic lichens are well-known indicators of air contamination. The chemical composition of lichens is affected by the concentration of pollutants in the environment. Usually, researchers assess long-term variations in trace elements or other pollutants in the study area, or identify spatial features of air contamination. The aim of this study is to create a database of trace element concentrations in the samples of the epiphytic lichen Hypogymnia physodes growing in the Kaliningrad region. The database can be used as a “reference point” for monitoring studies. Another objective is to identify the spatial patterns of iron, manganese, nickel, cadmium, silver, lead, strontium, rubidium, and calcium in the Kaliningrad region. With the help of a regular grid, samples of wild lichens were collected from pine and birch trees, 1.2–1.8 m from their bases, in August 2010. One- to two-year-old thalli were used in the chemical analysis. The metals Ag, Cd, Cu, Pb, Ni, Fe, Mn, and Zn were determined by atomic absorption spectrometry AAS (Mn and Fe by flame atomic absorption FA AAS and the others by electro thermal atomisation ETA AAS); the elements Sr, Rb, and Ca were determined by X-ray fluorescence. The concentration of metals in the western coastal area (the Sambian or Kaliningrad Peninsula) is higher than it is in the central and eastern parts of the region. Principal component factor analysis was carried out to detect and characterise different pollution sources. The authors examined the features of spatial distribution of trace elements. The prevailing wind direction is between south and west; therefore, the highest concentrations of trace elements were found on the Sambian peninsula and on the coasts of the Vistula and Curonian Lagoons. The chemical composition of lichens on the Sambian peninsula may have developed under the impact of both local pollution sources—vehicles, thermal energy facilities, and ports—and such factors as trans-boundary traffic and sea spray.
The Architecture of the Rag GTPase Signaling Network
Authors: Nicastro Raffaele , Sardu Alessandro , Panchaud Nicolas , De Virgilio Claudio
Published: July 2017

The evolutionarily conserved target of rapamycin complex 1 (TORC1) couples an array of intra- and extracellular stimuli to cell growth, proliferation and metabolism, and its deregulation is associated with various human pathologies such as immunodeficiency, epilepsy, and cancer. Among the diverse stimuli impinging on TORC1, amino acids represent essential input signals, but how they control TORC1 has long remained a mystery. The recent discovery of the Rag GTPases, which assemble as heterodimeric complexes on vacuolar/lysosomal membranes, as central elements of an amino acid signaling network upstream of TORC1 in yeast, flies, and mammalian cells represented a breakthrough in this field. Here, we review the architecture of the Rag GTPase signaling network with a special focus on structural aspects of the Rag GTPases and their regulators in yeast and highlight both the evolutionary conservation and divergence of the mechanisms that control Rag GTPases.
Thermal and Economic Analysis of Renovation Strategies for a Historic Building in Mediterranean Area
Authors: Cirami Simona , Evola Gianpiero , Gagliano Antonio , Margani Giuseppe
Published: July 2017

Around 30% of the European building stock was built before 1950, when no regulations about energy efficiency were in force. Since only a small part of them has been renovated by now, the energy performance of this building stock is on average quite poor, resulting in a significant impact on the energy balance of European countries, as confirmed by data published by ISTAT (Italian National Statistical Institute). However, energy retrofit in historic edifices is a quite demanding issue as any intervention must take into account the need to preserve existing building materials and appearances while also allowing reversibility and low invasiveness. As an example, in these buildings it is not possible to apply an ETICS (External Thermal Insulation Composite System), since this would alter the historic and architectural value of the façade. On the other hand, internal insulation would have the drawback of reducing the net useful floor area, which also implies a loss of economic value. Moreover, internal insulation may induce overheating risks and mold formation. In this paper, all these issues are investigated with reference to an existing historic building located in southern Italy, showing that a retrofit strategy aimed at energy savings and cost-effectiveness is still possible if suitable materials and solutions are adopted.
Methods to Monitor and Quantify Autophagy in the Social Amoeba Dictyostelium discoideum
Authors: Domínguez-Martín Eunice , Cardenal-Muñoz Elena , King Jason , Soldati Thierry , Coria Roberto , Escalante Ricardo
Published: July 2017

Autophagy is a eukaryotic catabolic pathway that degrades and recycles cellular components to maintain homeostasis. It can target protein aggregates, superfluous biomolecular complexes, dysfunctional and damaged organelles, as well as pathogenic intracellular microbes. Autophagy is a dynamic process in which the different stages from initiation to final degradation of cargo are finely regulated. Therefore, the study of this process requires the use of a palette of techniques, which are continuously evolving and whose interpretation is not trivial. Here, we present the social amoeba Dictyostelium discoideum as a relevant model to study autophagy. Several methods have been developed based on the tracking and observation of autophagosomes by microscopy, analysis of changes in expression of autophagy genes and proteins, and examination of the autophagic flux with various techniques. In this review, we discuss the pros and cons of the currently available techniques to assess autophagy in this organism.
Multidimensional-DSP Beamformers Using the ROACH-2 FPGA Platform
Authors: Seneviratne Vishwa , Madanayake Arjuna , Bruton Len
Published: July 2017

Antenna array-based multi-dimensional infinite-impulse response (IIR) digital beamformers are employed in a multitude of radio frequency (RF) applications ranging from electronically-scanned radar, radio telescopes, long-range detection and target tracking. A method to design 3D IIR beam filters using 2D IIR beam filters is described. A cascaded 2D IIR beam filter architecture is proposed based on systolic array architecture as an alternative for an existing radar application. Differential-form transfer function and polyphase structures are employed in the design to gain an increase in the speed of operation to gigahertz range. The feasibility of practical implementation of a 4-phase polyphase 2D IIR beam filter is explored. A digital hardware prototype is designed, implemented and tested using a ROACH-2 Field Programmable Gate Array (FPGA) platform fitted with a Xilinx Virtex-6 SX475T FPGA chip and multi-input analog-to-digital converters (ADC) boards set to a maximum sampling rate of 960 MHz. The article describes a method to build a 3D IIR beamformer using polyphase structures. A comparison of technical specifications of an existing radar application based on phased-array and the proposed 3D IIR beamformer is also explained to illustrate the proposed method to be a better alternative for such applications.
Research on Inverter Integrated Reactive Power Control Strategy in the Grid-Connected PV Systems
Authors: Li Hua , Wen Che , Chao Kuei-Hsiang , Li Ling-Ling
Published: July 2017

In order to solve the problems caused by the susceptibility to changing weather conditions and the complex load conditions of photovoltaic (PV) systems, and the fact a single target inverter control strategy cannot effectively mitigate large voltage fluctuations at point of common coupling (PCC), an integrated reactive power control strategy for PV inverters is proposed. According to the weather and load conditions, this strategy is divided into four control modes: normal operation control mode, reverse power control mode, cloudy control mode and night control mode. The four control modes switch between each other under the specific switching rules to ensure an appropriate quantity of reactive power injection or consumption in the PV inverter. The effects of the four control modes in isolated operation were significant. The deviation rate of PCC voltage from its standard value was mitigated at about 2% under the integrated control strategy. Therefore, the proposed control strategy has a high application value in improving power quality and maximizing utilization of PV inverters.
Using Latent Semantic Analysis to Identify Research Trends in OpenStreetMap
Authors: Sehra Sukhjit , Singh Jaiteg , Rai Hardeep
Published: July 2017

OpenStreetMap (OSM), based on collaborative mapping, has become a subject of great interest to the academic community, resulting in a considerable body of literature produced by many researchers. In this paper, we use Latent Semantic Analysis (LSA) to help identify the emerging research trends in OSM. An extensive corpus of 485 academic abstracts of papers published during the period 2007–2016 was used. Five core research areas and fifty research trends were identified in this study. In addition, potential future research directions have been provided to aid geospatial information scientists, technologists and researchers in undertaking future OSM research.
Improving Processing and Performance of Pure Lignin Carbon Fibers through Hardwood and Herbaceous Lignin Blends
Authors: Hosseinaei Omid , Harper David , Bozell Joseph , Rials Timothy
Published: July 2017

Lignin/lignin blends were used to improve fiber spinning, stabilization rates, and properties of lignin-based carbon fibers. Organosolv lignin from Alamo switchgrass (Panicum virgatum) and yellow poplar (Liriodendron tulipifera) were used as blends for making lignin-based carbon fibers. Different ratios of yellow poplar:switchgrass lignin blends were prepared (50:50, 75:25, and 85:15 w/w). Chemical composition and thermal properties of lignin samples were determined. Thermal properties of lignins were analyzed using thermogravimetric analysis and differential scanning calorimetry. Thermal analysis confirmed switchgrass and yellow poplar lignin form miscible blends, as a single glass transition was observed. Lignin fibers were produced via melt-spinning by twin-screw extrusion. Lignin fibers were thermostabilized at different rates and subsequently carbonized. Spinnability of switchgrass lignin markedly improved by blending with yellow poplar lignin. On the other hand, switchgrass lignin significantly improved thermostabilization performance of yellow poplar fibers, preventing fusion of fibers during fast stabilization and improving mechanical properties of fibers. These results suggest a route towards a 100% renewable carbon fiber with significant decrease in production time and improved mechanical performance.
An In Vitro Potency Assay for Monitoring the Immunomodulatory Potential of Stromal Cell-Derived Extracellular Vesicles
Authors: Pachler Karin , Ketterl Nina , Desgeorges Alexandre , Dunai Zsuzsanna , Laner-Plamberger Sandra , Streif Doris , Strunk Dirk , Rohde Eva , Gimona Mario
Published: July 2017

The regenerative and immunomodulatory activity of mesenchymal stromal cells (MSCs) is partially mediated by secreted vesicular factors. Extracellular vesicles (EVs) exocytosed by MSCs are gaining increased attention as prospective non-cellular therapeutics for a variety of diseases. However, the lack of suitable in vitro assays to monitor the therapeutic potential of EVs currently restricts their application in clinical studies. We have evaluated a dual in vitro immunomodulation potency assay that reproducibly reports the inhibitory effect of MSCs on induced T-cell proliferation and the alloantigen-driven mixed leukocyte reaction of pooled peripheral blood mononuclear cells in a dose-dependent manner. Phytohemagglutinin-stimulated T-cell proliferation was inhibited by MSC-derived EVs in a dose-dependent manner comparable to MSCs. In contrast, inhibition of alloantigen-driven mixed leukocyte reaction was only observed for MSCs, but not for EVs. Our results support the application of a cell-based in vitro potency assay for reproducibly determining the immunomodulatory potential of EVs. Validation of this assay can help establish reliable release criteria for EVs for future clinical studies.
Histone Deacetylase Inhibitors as Anticancer Drugs
Authors: Eckschlager Tomas , Plch Johana , Stiborova Marie , Hrabeta Jan
Published: July 2017

Carcinogenesis cannot be explained only by genetic alterations, but also involves epigenetic processes. Modification of histones by acetylation plays a key role in epigenetic regulation of gene expression and is controlled by the balance between histone deacetylases (HDAC) and histone acetyltransferases (HAT). HDAC inhibitors induce cancer cell cycle arrest, differentiation and cell death, reduce angiogenesis and modulate immune response. Mechanisms of anticancer effects of HDAC inhibitors are not uniform; they may be different and depend on the cancer type, HDAC inhibitors, doses, etc. HDAC inhibitors seem to be promising anti-cancer drugs particularly in the combination with other anti-cancer drugs and/or radiotherapy. HDAC inhibitors vorinostat, romidepsin and belinostat have been approved for some T-cell lymphoma and panobinostat for multiple myeloma. Other HDAC inhibitors are in clinical trials for the treatment of hematological and solid malignancies. The results of such studies are promising but further larger studies are needed. Because of the reversibility of epigenetic changes during cancer development, the potency of epigenetic therapies seems to be of great importance. Here, we summarize the data on different classes of HDAC inhibitors, mechanisms of their actions and discuss novel results of preclinical and clinical studies, including the combination with other therapeutic modalities.
Independent Preharvest Applications of Methyl Jasmonate and Chitosan Elicit Differential Upregulation of Defense-Related Genes with Reduced Incidence of Gray Mold Decay during Postharvest Storage of Fragaria chiloensis Fruit
Authors: Saavedra Gabriela , Sanfuentes Eugenio , Figueroa Pablo , Figueroa Carlos
Published: July 2017

The Chilean strawberry (Fragaria chiloensis) fruit has interesting organoleptic properties, but its postharvest life is affected by gray mold decay caused by Botrytis cinerea. The effect of preharvest applications of methyl jasmonate (MeJA) or chitosan on the molecular defense-related responses and protection against gray mold decay were investigated in Chilean strawberry fruit during postharvest storage. Specifically, we inoculated harvested fruit with B. cinerea spores and studied the expression of genes encoding for the pathogenesis-related (PR) proteins β-1,3-glucanases (FcBG2-1, FcBG2-2 and FcBG2-3) and chitinases (FcCHI2-2 and FcCHI3-1), and for polygalacturonase inhibiting proteins (FcPGIP1 and FcPGIP2) at 0, 2, 24, 48, and 72 h post inoculation (hpi). Remarkably, MeJA- and chitosan-treated fruit exhibited a lower incidence of B. cinerea infection than the control-treated at 48 and 72 hpi. At the molecular level, both are efficient elicitors for priming in F. chiloensis fruit since we observed an upregulation of the FcBG2-1, FcBG2-3, FcPGIP1, and FcPGIP2 at 0 hpi. Moreover, a chitosan-mediated upregulation of FcPGIPs at early times post inoculation (2–24 hpi) and MeJA upregulated FcBGs (24–72 hpi) and FcPGIP1 at later times could contribute to reduce B. cinerea incidence by differential upregulation of defense genes. We concluded that preharvest applications of MeJA or chitosan had a long-lasting effect on the reduction of B. cinerea incidence during postharvest as well as an enhancer effect on the induction of PR and PGIP gene expression.
Lipotoxicity-Induced PRMT1 Exacerbates Mesangial Cell Apoptosis via Endoplasmic Reticulum Stress
Authors: Park Min-Jung , Han Ho , Kim Dong-il
Published: July 2017

Lipotoxicity-induced mesangial cell apoptosis is implicated in the exacerbation of diabetic nephropathy (DN). Protein arginine methyltransferases (PRMTs) have been known to regulate a variety of biological functions. Recently, it was reported that PRMT1 expression is increased in proximal tubule cells under diabetic conditions. However, their roles in mesangial cells remain unexplored. Thus, we examined the pathophysiological roles of PRMTs in mesangial cell apoptosis. Treatment with palmitate, which mimics cellular lipotoxicity, induced mesangial cell apoptosis via protein kinase RNA-like endoplasmic reticulum kinase (PERK) and ATF6-mediated endoplasmic reticulum (ER) stress signaling. Palmitate treatment increased PRMT1 expression and activity in mesangial cells as well. Moreover, palmitate-induced ER stress activation and mesangial cell apoptosis was diminished by PRMT1 knockdown. In the mice study, high fat diet-induced glomerular apoptosis was attenuated in PRMT1 haploinsufficient mice. Together, these results provide evidence that lipotoxicity-induced PRMT1 expression promotes ER stress-mediated mesangial cell apoptosis. Strategies to regulate PRMT1 expression or activity could be used to prevent the exacerbation of DN.
Designing the Health-related Internet of Things: Ethical Principles and Guidelines
Authors: Mittelstadt Brent
Published: July 2017

The conjunction of wireless computing, ubiquitous Internet access, and the miniaturisation of sensors have opened the door for technological applications that can monitor health and well-being outside of formal healthcare systems. The health-related Internet of Things (H-IoT) increasingly plays a key role in health management by providing real-time tele-monitoring of patients, testing of treatments, actuation of medical devices, and fitness and well-being monitoring. Given its numerous applications and proposed benefits, adoption by medical and social care institutions and consumers may be rapid. However, a host of ethical concerns are also raised that must be addressed. The inherent sensitivity of health-related data being generated and latent risks of Internet-enabled devices pose serious challenges. Users, already in a vulnerable position as patients, face a seemingly impossible task to retain control over their data due to the scale, scope and complexity of systems that create, aggregate, and analyse personal health data. In response, the H-IoT must be designed to be technologically robust and scientifically reliable, while also remaining ethically responsible, trustworthy, and respectful of user rights and interests. To assist developers of the H-IoT, this paper describes nine principles and nine guidelines for ethical design of H-IoT devices and data protocols.
Information Mining from Heterogeneous Data Sources: A Case Study on Drought Predictions
Authors: Demisse Getachew , Tadesse Tsegaye , Atnafu Solomon , Hill Shawndra , Wardlow Brian , Bayissa Yared , Shiferaw Andualem
Published: July 2017

The objective of this study was to develop information mining methodology for drought modeling and predictions using historical records of climate, satellite, environmental, and oceanic data. The classification and regression tree (CART) approach was used for extracting drought episodes at different time-lag prediction intervals. Using the CART approach, a number of successful model trees were constructed, which can easily be interpreted and used by decision makers in their drought management decisions. The regression rules produced by CART were found to have correlation coefficients from 0.71–0.95 in rules-alone modeling. The accuracies of the models were found to be higher in the instance and rules model (0.77–0.96) compared to the rules-alone model. From the experimental analysis, it was concluded that different combinations of the nearest neighbor and committee models significantly increase the performances of CART drought models. For more robust results from the developed methodology, it is recommended that future research focus on selecting relevant attributes for slow-onset drought episode identification and prediction.
Innate Immune Responses to Cryptococcus
Authors: Heung Lena
Published: July 2017

Cryptococcus species are encapsulated fungi found in the environment that predominantly cause disease in immunocompromised hosts after inhalation into the lungs. Even with contemporary antifungal regimens, patients with cryptococcosis continue to have high morbidity and mortality rates. The development of more effective therapies may depend on our understanding of the cellular and molecular mechanisms by which the host promotes sterilizing immunity against the fungus. This review will highlight our current knowledge of how Cryptococcus, primarily the species C. neoformans, is sensed by the mammalian host and how subsequent signaling pathways direct the anti-cryptococcal response by effector cells of the innate immune system.
In Vivo Damage of the Head-Neck Junction in Hard-on-Hard Total Hip Replacements: Effect of Femoral Head Size, Metal Combination, and 12/14 Taper Design
Authors: Baleani Massimiliano , Erani Paolo , Bordini Barbara , Zuccheri Federica , Mąkosa Mateusz , De Pasquale Dalila , Beraudi Alina , Stea Susanna
Published: July 2017

Recently, concerns have been raised about the potential effect of head-neck junction damage products at the local and systemic levels. Factors that may affect this damage process have not been fully established yet. This study investigated the possible correlations among head-neck junction damage level, implant design, material combination, and patient characteristics. Head-neck junctions of 148 retrieved implants were analysed, including both ceramic-on-ceramic (N = 61) and metal-on-metal (N = 87) bearings. In all cases, the male taper was made of titanium alloy. Damage was evaluated using a four-point scoring system based on damage morphology and extension. Patient age at implantation, implantation time, damage risk factor, and serum ion concentration were considered as independent potential predicting variables. The damage risk factor summarises head-neck design characteristics and junction loading condition. Junction damage correlated with both implantation time and damage factor risk when the head was made of ceramic. A poor correlation was found when the head was made of cobalt alloy. The fretting-corrosion phenomenon seemed mainly mechanically regulated, at least when cobalt alloy components were not involved. When a component was made of cobalt alloy, the role of chemical phenomena increased, likely becoming, over implantation time, the damage driving phenomena of highly stressed junctions.
Mechanical Performance of Asphalt Mortar Containing Hydrated Lime and EAFSS at Low and High Temperatures
Authors: Moon Ki Hoon , Falchetto Augusto Cannone , Wang Di , Riccardi Chiara , Wistuba Michael P.
Published: July 2017

In this paper, the possibility of improving the global response of asphalt materials for pavement applications through the use of hydrated lime and Electric Arc-Furnace Steel Slag (EAFSS) was investigated. For this purpose, a set of asphalt mortars was prepared by mixing two different asphalt binders with fine granite aggregate together with hydrated lime or EAFSS at three different percentages. Bending Beam Rheometer (BBR) creep tests and Dynamic Shear Rheometer (DSR) complex modulus tests were performed to evaluate the material response both at low and high temperature. Then, the rheological Huet model was fitted to the BBR creep results for estimating the impact of filler content on the model parameters. It was found that an addition of hydrated lime and EAFSS up to 10% and 5%, respectively, results in satisfactory low-temperature performance with a substantial improvement of the high-temperature behavior.
24-Methyl-Cholesta-5,24(28)-Diene-3β,19-diol-7β-Monoacetate Inhibits Human Small Cell Lung Cancer Growth In Vitro and In Vivo via Apoptosis Induction
Authors: Chung Ting-Wen , Su Jui-Hsin , Lin Chi-Chen , Li Yi-Rong , Chao Ya-Hsuan , Lin Sheng-Hao , Chan Hong-Lin
Published: July 2017

24-methyl-cholesta-5,24(28)-diene-3β,19-diol-7β-monoacetate (MeCDDA) is a natural steroid compound isolated from a wild-type soft coral (Nephthea erecta). The present study aimed to investigate the anti-small cell lung cancer (SCLC) effects of MeCDDA in vitro and in vivo, as well as to elucidate its underlying mechanism. Our results indicated that H1688 and H146 cells show relevant sensitivity to MeCDDA, and the exposure to MeCDDA in SCLC cells caused dose-dependent growth inhibitory responses. In addition, MeCDDA treatment promoted cell apoptosis and increased the activities of caspases in H1688 cells, reducing the mitochondrial membrane potential and stimulating the release of cytochrome c into the cytosol. Along with the increase in Bax expression and reduction in Bcl-2, the MeCDDA treatment also significantly decreased Akt and mTOR phosphorylation. Finally, MeCDDA treatment in the mouse xenograft model of H1688 cells exhibited significant inhibition of tumor growth, corroborating MeCDDA as a potential pre-clinical candidate for the treatment of SCLC. Overall, our results demonstrate that the cytotoxic effects of MeCDDA towards H1688 and H146 cells, possibly through the activation of the mitochondrial apoptotic pathway and inhibition of the PI3K/Akt/mTOR pathway, merit further studies for its possible clinical application in chemotherapy.
Simultaneous Removal of Hg(II) and Phenol Using Functionalized Activated Carbon Derived from Areca Nut Waste
Authors: Lalhmunsiama  , Lee Seung , Choi Suk , Tiwari Diwakar
Published: July 2017

Areca nut waste was utilized to obtain high surface area activated carbon (AC), and it was further functionalized with succinic anhydride under microwave irradiation. The surface morphology and surface functional groups of the materials were discussed with the help of scanning electron microscope(SEM) images and fourier transform infra-red (FT-IR) analysis. The specific surface area of the AC and functionalized-AC was obtained by the Brunauer-Emmett-Teller (BET) method, and found to be 367.303 and 308.032 m2/g, respectively. Batch experiments showed that higher pH favoured the removal of Hg(II), whereas the phenol removal was slightly affected by the changes in the solution pH. The kinetic data followed pseudo-first order kinetic model, and intra-particle diffusion played a significant role in the removal of both pollutants. The maximum sorption capacity of Hg(II) and phenol were evaluated using Langmuir adsorption isotherms, and found to be 11.23 and 5.37 mg/g, respectively. The removal of Hg(II) was significantly suppressed in the presence of chloride ions due to the formation of a HgCl2 species. The phenol was specifically adsorbed, forming the donor–acceptor complexes or π–π electron interactions at the surface of the solid. Further, a fixed-bed column study was conducted for both Hg(II) and phenol. The loading capacity of the column was estimated using the nonlinear Thomas equation, and found to be 2.49 and 2.70 mg/g, respectively. Therefore, the study showed that functionalized AC obtained from areca nut waste could be employed as a sustainable adsorbent for the simultaneous removal of Hg(II) and phenol from polluted water.
DC Microplasma Jet for Local a:C-H Deposition Operated in SEM Chamber
Authors: Matra Khanit , Furuta Hiroshi , Hatta Akimitsu
Published: July 2017

A DC micro plasma jet for local micro deposition of a:C-H film in the ambient vacuum of scanning electron microscope (SEM) chamber is proposed. Acetylene (C2H2) gas was locally fed into the chamber through an orifice shaped gas nozzle (OGN) at 6.6 sccm in flow rate by applying 80 kPa-inlet pressure with an additional direct pumping system equipped on the SEM chamber. As a cathode, a cut of n-type silicon (Si) wafer was placed right in front of the OGN at 200 μm gap distance. By applying a positive DC voltage to the OGN, C2H2 plasma was generated locally between the electrodes. During discharge, the voltage increased and the current decreased due to deposition of insulating film on the Si wafer with resulting in automatic termination of discharge at the constant source voltage. A symmetric mountain-shaped a:C-H film of 5 μm height was deposited at the center by operation for 15 s. Films were deposited with variation of gas flow rate, gap distance, voltage and current, and deposition time. The films were directly observed by SEM and analyzed by surface profiler and by Raman spectroscopy.
The Influence of Common Monovalent and Divalent Chlorides on Chalcopyrite Flotation
Authors: Li Yubiao , Li Wanqing , Xiao Qing , He Nan , Ren Zijie , Lartey Clement , Gerson Andrea
Published: July 2017

Much attention has been paid to the flotation of chalcopyrite using saline seawater. However, the influence of salt ions on mineral flotation is complex, and different salts may play various roles—either beneficial or detrimental. This study investigated the effects of common chlorides (Cl−) of Na+, K+, Mg2+, and Ca2+ in seawater on chalcopyrite floatability. The presence of Na+, K+, and Ca2+ resulted in greater chalcopyrite recovery, with this effect being more pronounced for the monovalent cations. In contrast, the addition of Mg2+ resulted in decreased chalcopyrite flotation efficiency. Contact angle measurements showed that the presence of monovalent cations increased the hydrophobicity of the chalcopyrite surface, while the presence of divalent cations reduced its hydrophobicity, depending on the concentration. Zeta potential, pulp species, and X-ray photoelectron spectroscopy (XPS) cross-confirmed the precipitation of Mg(OH)2 on the chalcopyrite surface when Mg concentration was 10−2 M and pulp pH was 10.
The Activation Mechanism of Bi3+ Ions to Rutile Flotation in a Strong Acidic Environment
Authors: Xiao Wei , Cao Pan , Liang Qiannan , Peng Hong , Zhao Hongbo , Qin Wenqing , Qiu Guanzhou , Wang Jun
Published: July 2017

Lead hydroxyl compounds are known as rutile flotation of the traditional activated component, but the optimum pH range for flotation is 2–3 using styryl phosphoric acid (SPA) as collector, without lead hydroxyl compounds in slurry solution. In this study, Bi3+ ions as a novel activator was investigated. The results revealed that the presence of Bi3+ ions increased the surface potential, due to the specific adsorption of hydroxyl compounds, which greatly increases the adsorption capacity of SPA on the rutile surface. Bi3+ ions increased the activation sites through the form of hydroxyl species adsorbing on the rutile surface and occupying the steric position of the original Ca2+ ions. The proton substitution reaction occurred between the hydroxyl species of Bi3+ ions (Bi(OH)n+(3−n)) and the hydroxylated rutile surface, producing the compounds of Ti-O-Bi2+. The micro-flotation tests results suggested that Bi3+ ions could improve the flotation recovery of rutile from 61% to 90%, and from 61% to 64% for Pb2+ ions.
Interleukin-10 Conjugation to Carboxylated PVP-Coated Silver Nanoparticles for Improved Stability and Therapeutic Efficacy
Authors: Baganizi Dieudonné , Nyairo Elijah , Duncan Skyla , Singh Shree , Dennis Vida
Published: July 2017

Interleukin-10 (IL-10) is a key anti-inflammatory and immunosuppressive cytokine and therefore represents a potential therapeutic agent especially in inflammatory diseases. However, despite its proven therapeutic efficacy, its short half-life and proteolytic degradation in vivo combined with its low storage stability have limited its therapeutic use. Strategies have been developed to overcome most of these shortcomings, including in particular bioconjugation with stabilizing agents such as polyethylene glycol (PEG) and poly (vinylpyrolidone) (PVP), but so far these have had limited success. In this paper, we present an alternative method consisting of bioconjugating IL-10 to PVP-coated silver nanoparticles (Ag-PVPs) in order to achieve its storage stability by preventing denaturation and to improve its anti-inflammatory efficacy. Silver nanoparticles capped with a carboxylated PVP were produced and further covalently conjugated with IL-10 protein by carbodiimide crosslinker chemistry. The IL-10 conjugated Ag-PVPs exhibited increased stability and anti-inflammatory effectiveness in vitro. This study therefore provides a novel approach to bioconjugating PVP-coated silver nanoparticles with therapeutic proteins, which could be useful in drug delivery and anti-inflammatory therapies.
Precipitation and Neutralization of Heparin from Different Sources by Protamine Sulfate
Authors: Hogwood John , Mulloy Barbara , Gray Elaine
Published: July 2017

Current therapeutic unfractionated heparin available in Europe and US is of porcine mucosal origin. There is now interest, specifically in the US, to use bovine mucosa as an additional source for the production of heparin. The anticoagulant action of heparin can be neutralized by protamine sulfate, and in this study the ability of protamine to bind and neutralize the anticoagulant activities of heparin from porcine mucosa, bovine mucosa and bovine lung were assessed. Protamine sulfate was able to bind and precipitate similar amounts of heparins from different sources on a mass basis. However, differential amounts of anticoagulant activities were neutralized by protamine sulfate, with neutralization of porcine mucosa more effective than for bovine lung and bovine mucosa. For all heparins, potentiation of thrombin inhibition by antithrombin and heparin cofactor II was preferentially neutralized over antithrombin-mediated inhibition of factor Xa or plasma clotting time. Whole blood thromboelastography showed that neutralization by protamine sulfate was more effective than the antithrombin dependent thrombin inhibition assays indicated. While there was no absolute correlation between average or peak molecular weight of heparin samples and neutralization of anticoagulant activity, correlation was observed between proportions of material with high affinity to antithrombin, specific activities and neutralization of activity.
Enhancement of Thermal Diffusivity in Phase-Separated Bismaleimide/Poly(ether imide) Composite Films Containing Needle-Shaped ZnO Particles
Authors: Uchida Shoya , Ishige Ryohei , Ando Shinji
Published: July 2017

Phase-separated polymer blend composite films exhibiting high thermal diffusivity were prepared by blending a soluble polyimide (BPADA-MPD) and a bismaleimide (BMI) with needle-shaped zinc oxide (n-ZnO) particles followed by high-temperature curing at 250 °C. Images recorded with a field-emission scanning electron microscope (FE-SEM) equipped with wavelength-dispersive spectroscopy (WDS) demonstrated that the spontaneously separated phases in the composite films were aligned along the out-of-plane direction, and the n-ZnO particles were selectively incorporated into the BMI phase. The out-of-plane thermal diffusivity of the composite films was significantly higher than those of the previously reported composite films at lower filler contents. Based on wide-angle X-ray diffraction (WAXD) patterns and image analysis, the enhanced thermal diffusivity was attributed to the confinement of the anisotropically shaped particles and their nearly isotropic orientation in one phase of the composite films.
Accuracy Improvements in the Orientation of ALOS PRISM Images Using IOP Estimation and UCL Kepler Platform Model
Authors: Rodrigues Tiago , Mitishita Edson , Ferreira Luiz , Tommaselli Antonio
Published: July 2017

This paper presents a study that was conducted to determine the orientation of ALOS (Advanced Land Observing Satellite) PRISM (Panchromatic Remote-sensing Instrument for Stereo Mapping) triplet images, considering the estimation of interior orientation parameters (IOP) of the cameras and using the collinearity equations with the UCL (University College of London) Kepler platform model, which was adapted to use coordinates referenced to the Terrestrial Reference System ITRF97. The results of the experiments showed that the accuracies of 3D coordinates calculated using 3D photogrammetric intersection increased when the IOP were also estimated. The vertical accuracy was significantly better than the horizontal accuracy. The usability of the estimated IOP was tested to perform the bundle block adjustments of another neighbouring PRISM image triplet. The results in terms of 3D photogrammetric intersection were satisfactory and were close to those obtained in the IOP estimation experiment.
Predicting Vascular Plant Diversity in Anthropogenic Peatlands: Comparison of Modeling Methods with Free Satellite Data
Authors: Castillo-Riffart Ivan , Galleguillos Mauricio , Lopatin Javier , Perez-Quezada and Jorge F.
Published: July 2017

Peatlands are ecosystems of great relevance, because they have an important number of ecological functions that provide many services to mankind. However, studies focusing on plant diversity, addressed from the remote sensing perspective, are still scarce in these environments. In the present study, predictions of vascular plant richness and diversity were performed in three anthropogenic peatlands on Chiloé Island, Chile, using free satellite data from the sensors OLI, ASTER, and MSI. Also, we compared the suitability of these sensors using two modeling methods: random forest (RF) and the generalized linear model (GLM). As predictors for the empirical models, we used the spectral bands, vegetation indices and textural metrics. Variable importance was estimated using recursive feature elimination (RFE). Fourteen out of the 17 predictors chosen by RFE were textural metrics, demonstrating the importance of the spatial context to predict species richness and diversity. Non-significant differences were found between the algorithms; however, the GLM models often showed slightly better results than the RF. Predictions obtained by the different satellite sensors did not show significant differences; nevertheless, the best models were obtained with ASTER (richness: R2 = 0.62 and %RMSE = 17.2, diversity: R2 = 0.71 and %RMSE = 20.2, obtained with RF and GLM respectively), followed by OLI and MSI. Diversity obtained higher accuracies than richness; nonetheless, accurate predictions were achieved for both, demonstrating the potential of free satellite data for the prediction of relevant community characteristics in anthropogenic peatland ecosystems.
Hybrid Optical Unobtrusive Blood Pressure Measurements
Authors: Zhang Guangfei , Shan Caifeng , Kirenko Ihor , Long Xi , Aarts Ronald
Published: July 2017

Blood pressure (BP) is critical in diagnosing certain cardiovascular diseases such as hypertension. Some previous studies have proved that BP can be estimated by pulse transit time (PTT) calculated by a pair of photoplethysmography (PPG) signals at two body sites. Currently, contact PPG (cPPG) and imaging PPG (iPPG) are two feasible ways to obtain PPG signals. In this study, we proposed a hybrid system (called the ICPPG system) employing both methods that can be implemented on a wearable device, facilitating the measurement of BP in an inconspicuous way. The feasibility of the ICPPG system was validated on a dataset with 29 subjects. It has been proved that the ICPPG system is able to estimate PTT values. Moreover, the PTT measured by the new system shows a correlation on average with BP variations for most subjects, which could facilitate a new generation of BP measurement using wearable and mobile devices.
An Infrared Actin Probe for Deep-Cell Electroporation-Based Single-Molecule Speckle (eSiMS) Microscopy
Authors: Yamashiro Sawako , Watanabe Naoki
Published: July 2017

Single-molecule speckle (SiMS) microscopy is a powerful method to directly elucidate biochemical reactions in live cells. However, since the signal from an individual fluorophore is extremely faint, the observation area by epi-fluorescence microscopy is restricted to the thin cell periphery to reduce autofluorescence, or only molecules near the plasma membrane are visualized by total internal reflection fluorescence (TIRF) microscopy. Here, we introduce a new actin probe labeled with near infrared (NIR) emissive CF680R dye for easy-to-use, electroporation-based SiMS microscopy (eSiMS) for deep-cell observation. CF680R-labeled actin (CF680R-actin) incorporated into actin structures and showed excellent brightness and photostability suitable for single-molecule imaging. Importantly, the intensity of autofluorescence with respect to SiMS brightness was reduced to approximately 13% compared to DyLight 550-labeled actin (DL550-actin). CF680R-actin enabled the monitoring of actin SiMS in actomyosin bundles associated with adherens junctions (AJs) located at 3.5–4 µm above the basal surfaces of epithelial monolayers. These favorable properties of CF680R-actin extend the application of eSiMS to actin turnover and flow analyses in deep cellular structures.
Moving Object Detection in Heterogeneous Conditions in Embedded Systems
Authors: Garbo Alessandro , Quer Stefano
Published: July 2017

This paper presents a system for moving object exposure, focusing on pedestrian detection, in external, unfriendly, and heterogeneous environments. The system manipulates and accurately merges information coming from subsequent video frames, making small computational efforts in each single frame. Its main characterizing feature is to combine several well-known movement detection and tracking techniques, and to orchestrate them in a smart way to obtain good results in diversified scenarios. It uses dynamically adjusted thresholds to characterize different regions of interest, and it also adopts techniques to efficiently track movements, and detect and correct false positives. Accuracy and reliability mainly depend on the overall receipt, i.e., on how the software system is designed and implemented, on how the different algorithmic phases communicate information and collaborate with each other, and on how concurrency is organized. The application is specifically designed to work with inexpensive hardware devices, such as off-the-shelf video cameras and small embedded computational units, eventually forming an intelligent urban grid. As a matter of fact, the major contribution of the paper is the presentation of a tool for real-time applications in embedded devices with finite computational (time and memory) resources. We run experimental results on several video sequences (both home-made and publicly available), showing the robustness and accuracy of the overall detection strategy. Comparisons with state-of-the-art strategies show that our application has similar tracking accuracy but much higher frame-per-second rates.