bluuebunny/crossref_metadata_2025 huggingface dataset:
Antibiofilm Activity of Epoxy/Ag-TiO2 Polymer Nanocomposite Coatings against Staphylococcus Aureus and Escherichia Coli
Authors: M. Santhosh , Natarajan Kandasamy
Published: April 2015

Dispersion of functional inorganic nano-fillers like TiO2 within polymer matrix is known to impart excellent photobactericidal activity to the composite. Epoxy resin systems with Ag+ ion doped TiO2 can have combination of excellent biocidal characteristics of silver and the photocatalytic properties of TiO2. The inorganic antimicrobial incorporation into an epoxy polymeric matrix was achieved by sonicating laboratory-made nano-scale anatase TiO2 and Ag-TiO2 into the industrial grade epoxy resin. The resulting epoxy composite had ratios of 0.5–2.0 wt% of nano-filler content. The process of dispersion of Ag-TiO2 in the epoxy resin resulted in concomitant in situ synthesis of silver nanoparticles due to photoreduction of Ag+ ion. The composite materials were characterized by DSC and SEM. The glass transition temperature (Tg) increased with the incorporation of the nanofillers over the neat polymer. The materials synthesized were coated on glass petri dish. Anti-biofilm property of coated material due to combined release of biocide, and photocatalytic activity under static conditions in petri dish was evaluated against Staphylococcus aureus ATCC6538 and Escherichia coli K-12 under UV irradiation using a crystal violet binding assay. Prepared composite showed significant inhibition of biofilm development in both the organisms. Our studies indicate that the effective dispersion and optimal release of biocidal agents was responsible for anti-biofilm activity of the surface. The reported thermoset coating materials can be used as bactericidal surfaces either in industrial or healthcare settings to reduce the microbial loads.
Implications of Non-Differentiable Entropy on a Space-Time Manifold
Authors: Agop Maricel , Gavriluţ Alina , Ştefan Gavril , Doroftei Bogdan
Published: April 2015

Assuming that the motions of a complex system structural units take place on continuous, but non-differentiable curves of a space-time manifold, the scale relativity model with arbitrary constant fractal dimension (the hydrodynamic and wave function versions) is built. For non-differentiability through stochastic processes of the Markov type, the non-differentiable entropy concept on a space-time manifold in the hydrodynamic version and its correspondence with motion variables (energy, momentum, etc.) are established. Moreover, for the same non-differentiability type, through a scale resolution dependence of a fundamental length and wave function independence with respect to the proper time, a non-differentiable Klein–Gordon-type equation in the wave function version is obtained. For a phase-amplitude functional dependence on the wave function, the non-differentiable spontaneous symmetry breaking mechanism implies pattern generation in the form of Cooper non-differentiable-type pairs, while its non-differentiable topology implies some fractal logic elements (fractal bit, fractal gates, etc.).
Evaluating Physical and Perceptual Responses to Exergames in Chinese Children
Authors: Lau Patrick , Liang Yan , Lau Erica , Choi Choung-Rak , Kim Chang-Gyun , Shin Myung-Soo
Published: April 2015

Purpose: The primary objective of this study was to examine whether exergames could help children reach the recommendations for PA and cardiorespiratory fitness regarding exercise intensity. Differences in perceived physical exertion, EE, VO2, and HR between normal weight (NW) and overweight (OW) children participating in exergames were also examined. Methods: Twenty-one children (age: 10.45 ± 0.88) were assessed for EE, VO2 and HR during rest, in a maximal treadmill test, and while playing different exergames. Ratings of perceived exertion (RPE) (category range: 0 to 10) were also measured during exergaming. Three types of exergames were examined: running, table tennis, and dancing. These games were either performed on a Chinese game console, I-Dong, or another well-developed Western game console (Sony PlayStation 3 or Nintendo Wii). Results: Exergaming resulted in EE (kcal/min) from 2.05–5.14, VO2 (mL/kg/min) from 9.98–25.54, and HR (beats per minute) from 98.05–149.66. Children reported RPE ranging from 1.29 to 5.29. The Chinese exergame, I-Dong Running, was the only game in which children reached a moderate intensity and met the recommended minimum VO2reserve (50%) for cardiorespiratory fitness. Conclusion: Exergames could provide alternative opportunities to enhance children’s physical activity. They could be used as light-to-moderate PA, and with exergames, children can even reach the recommended intensity for developing and maintaining cardiorespiratory fitness.
Chemical Characterisation of the Coarse and Fine Particulate Matter in the Environment of an Underground Railway System: Cytotoxic Effects and Oxidative Stress—A Preliminary Study
Authors: Spagnolo Anna , Ottria Gianluca , Perdelli Fernanda , Cristina Maria
Published: April 2015

Background: Exposure to the particulate matter produced in underground railway systems is arousing increasing scientific interest because of its health effects. The aim of our study was to evaluate the airborne concentrations of PM10 and three sub-fractions of PM2.5 in an underground railway system environment in proximity to platforms and in underground commercial areas within the system, and to compare these with the outdoor airborne concentrations. We also evaluated the metal components, the cytotoxic properties of the various fractions of particulate matter (PM) and their capacity to induce oxidative stress. Method: We collected the coarse fraction (5–10 µm) and the fine fractions (1–2.5 µm; 0.5–1 µm; 0.25–0.5 µm). Chemical characterisation was determined by means of spectrometry. Cytotoxicity and oxidative stress were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and Reactive Oxygen Species (ROS) assessment. Results: The concentrations of both PM10 and PM2.5 proved to be similar at the three sampling sites. Iron and other transition metals displayed a greater concentration at the subway platform than at the other two sites. The 2.5–10 µm and 1–2.5 µm fractions of PM from all three sampling sites determined a greater increase in ROS; the intensity of oxidative stress progressively declined as particle diameter diminished. Moreover, ROS concentrations were correlated with the concentrations of some transition metals, namely Mn, Cr, Ti, Fe, Cu, Zn, Ni and Mo. All particulate matter fractions displayed lower or similar ROS values between platform level and the outdoor air. Conclusions: The present study revealed that the underground railway environment at platform level, although containing higher concentrations of some particularly reactive metallic species, did not display higher cytotoxicity and oxidative stress levels than the outdoor air.
Emergency Hospital Visits in Association with Volcanic Ash, Dust Storms and Other Sources of Ambient Particles: A Time-Series Study in Reykjavík, Iceland
Authors: Carlsen Hanne , Gislason Thorarinn , Forsberg Bertil , Meister Kadri , Thorsteinsson Throstur , Jóhannsson Thorsteinn , Finnbjornsdottir Ragnhildur , Oudin Anna
Published: April 2015

Volcanic ash contributed significantly to particulate matter (PM) in Iceland following the eruptions in Eyjafjallajökull 2010 and Grímsvötn 2011. This study aimed to investigate the association between different PM sources and emergency hospital visits for cardiorespiratory causes from 2007 to 2012. Indicators of PM10 sources; “volcanic ash”, “dust storms”, or “other sources” (traffic, fireworks, and re-suspension) on days when PM10 exceeded the daily air quality guideline value of 50 µg/m3 were entered into generalized additive models, adjusted for weather, time trend and co-pollutants. The average number of daily emergency hospital visits was 10.5. PM10 exceeded the air quality guideline value 115 out of 2191 days; 20 days due to volcanic ash, 14 due to dust storms (two days had both dust storm and ash contribution) and 83 due to other sources. High PM10 levels from volcanic ash tended to be significantly associated with the emergency hospital visits; estimates ranged from 4.8% (95% Confidence Interval (CI): 0.6, 9.2%) per day of exposure in unadjusted models to 7.3% (95% CI: −0.4, 15.5%) in adjusted models. Dust storms were not consistently associated with daily emergency hospital visits and other sources tended to show a negative association. We found some evidence indicating that volcanic ash particles were more harmful than particles from other sources, but the results were inconclusive and should be interpreted with caution.
Characterization of the Recognition Specificity of BH2, a Monoclonal Antibody Prepared against the HLA-B27 Heavy Chain
Authors: Yu Hui-Chun , Huang Kuang-Yung , Lu Ming-Chi , Huang Hsien-Lu , Liu Wei-Ting , Lee Wen-Chien , Liu Su-Qin , Huang Hsien-Bin , Lai Ning-Sheng
Published: April 2015

BH2, a monoclonal antibody prepared against the denatured human leukocytic antigen-B27 heavy chain (HLA-B27 HC), can immunoprecipitate the misfolded HLA-B27 HC complexed with Bip in the endoplasmic reticulum and recognize the homodimerized HLA-B27 HC that is often observed on the cell membrane of patients suffered from ankylosing spondylitis (AS). However, the recognition specificity of BH2 toward the other molecules of HLA-B type and toward the different types of HLA molecules remained uncharacterized. In this study, we carried out the HLA-typing by using the Luminex Technology to characterize the recognition specificity of BH2 and analyzed the binding domain of HLA-B27 HC by BH2. Our results indicated that BH2 preferably binds to molecules of HLA-B and -C rather than HLA-A and the binding site is located within the α2 domain of HLA-B27 HC.
Revisiting the Formation and Tunable Dissociation of a [2]Pseudorotaxane Formed by Slippage Approach
Authors: Leung Ken , Lau Kwun-Ngai , Wong Wing-Yan
Published: April 2015

A new [2]pseudorotaxane DB24C8É1-H·PF6 with dibenzo[24]crown-8 (DB24C8) crown ether-dibenzylammonium (1-H·PF6) binding which was formed by slippage approach at different solvents and temperature, had been isolated and characterized by NMR spectroscopy and mass spectrometry. The [2]pseudorotaxane DB24C8É1-H·PF6 was stable at room temperature. The dissociation rate of [2]pseudorotaxane DB24C8É1-H·PF6 could be tuned by using different stimuli such as triethylamine (TEA)/diisopropylethylamine (DIPEA) and dimethyl sulfoxide (DMSO). In particular, the dissociation of [2]pseudorotaxane DB24C8É1-H·PF6 by an excess of TEA/DIPEA base mixture possessed a long and sustained, complete dissociation over 60 days. Other stimuli by DMSO possessed a relatively fast dissociation over 24 h.
Fibroblastic Transformation of Corneal Keratocytes by Rac Inhibition is Modulated by Extracellular Matrix Structure and Stiffness
Authors: Petroll W. , Lakshman Neema
Published: April 2015

The goal of this study was to investigate how alterations in extracellular matrix (ECM) biophysical properties modulate corneal keratocyte phenotypes in response to specific wound healing cytokines and Rho GTPases. Rabbit corneal keratocytes were plated within standard collagen matrices (2.5 mg/mL) or compressed collagen matrices (~100 mg/mL) and cultured in serum-free media, PDGF BB, IGF, FGF2 or TGFβ1, with or without the Rac1 inhibitor NSC23766 and/or the Rho kinase inhibitor Y-27632. After 1 to 4 days, cells were labeled for F-actin and imaged using confocal microscopy. Keratocytes within standard collagen matrices (which are highly compliant) maintained a dendritic phenotype following culture in serum-free media, PDGF, IGF and FGF, but developed stress fibers in TGFβ1. Keratocytes within compressed collagen (which has high stiffness and low porosity) maintained a dendritic phenotype following culture in serum-free media, PDGF and IGF, but developed stress fibers in both FGF and TGFβ1. The Rac inhibitor had no significant impact on growth factor responses in compliant matrices. Within compressed collagen matrices however, the Rac inhibitor induced fibroblastic transformation in serum-free media, PDGF and IGF. Fibroblast and myofibroblast transformation was blocked by Rho kinase inhibition. Overall, keratocyte growth factor responses appear to be regulated by both the interplay between Rho and Rac signaling, and the structural and mechanical properties of the ECM.
Agent-Based Models as “Interested Amateurs”
Authors: Johnson Peter
Published: April 2015

This paper proposes the use of agent-based models (ABMs) as “interested amateurs” in policy making, and uses the example of the SWAP model of soil and water conservation adoption to demonstrate the potential of this approach. Daniel Dennett suggests experts often talk past or misunderstand each other, seek to avoid offending each other or appearing ill-informed and generally err on the side of under-explaining a topic. Dennett suggests that these issues can be overcome by including “interested amateurs” in discussions between experts. In the context of land use policy debates, and policy making more generally, this paper suggests that ABMs have particular characteristics that make them excellent potential “interested amateurs” in discussions between our experts: policy stakeholders. This is demonstrated using the SWAP (Soil and Water Conservation Adoption) model, which was used with policy stakeholders in Ethiopia. The model was successful in focussing discussion, inviting criticism, dealing with sensitive topics and drawing out understanding between stakeholders. However, policy stakeholders were still hesitant about using such a tool. This paper reflects on these findings and attempts to plot a way forward for the use of ABMs as “interested amateurs” and, in the process, make clear the differences in approach to other participatory modelling efforts.
In Vitro Wear Testing of a CoCr-UHMWPE Finger Prosthesis with Hydroxyapatite Coated CoCr Stems
Authors: Naylor Andrew , Talwalkar Sumedh , Trail Ian , Joyce Thomas
Published: April 2015

A finger prosthesis consisting of a Cobalt-chromium (CoCr) proximal component and an Ultra-high-molecular-weight-polyethylene (UHMWPE) medial component (both mounted on hydroxyapatite coated stems) was evaluated to 5,000,000 cycles in an in vitro finger simulator. One “test” prosthesis was cycled through flexion-extension (90°–30°) with a dynamic load of 10 N, whilst immersed in a lubricant of dilute bovine serum. Additionally, a static load of 100 N was applied for 45 s every 3000 cycles to simulate a static gripping force. A second “control” prosthesis was immersed in the same lubricant to account for absorption. Gravimetric and Sa (3D roughness) measurements were taken at 1,000,000 cycle intervals. Micrographs and Sa values revealed negligible change to the CoCr surfaces after 5,000,000 cycles. The UHMWPE also exhibited no distinctive Sa trend, however the micrographs indicate that polishing occurred. Both the CoCr and UHMWPE test components progressively decreased in weight. The CoCr control component did not change in weight, whilst the UHMWPE component gained weight through absorption. To account for the disparity between surface and gravimetric results, the hydroxyapatite coatings were examined. Micrographs of the test stems revealed that the hydroxyapatite coating was partially removed, whilst the micrographs of the control stems exhibited a uniform coating.
Thermo-Hydrodynamic Analysis of a Plain Journal Bearing on the Basis of a New Mass Conserving Cavitation Algorithm
Authors: Alakhramsing Shivam , Van Ostayen Ron , Eling Rob
Published: April 2015

Accurate prediction of cavitation is an important feature in hydrodynamic bearing modeling. Especially for thermo-hydrodynamic modeling, it is crucial to use a mass-conservative cavitation algorithm. This paper introduces a new mass-conserving Reynolds cavitation algorithm, which provides fast convergence and easy implementation in finite element models. The proposed algorithm is based on a variable transformation for both the pressure and mass fraction, which is presented in the form of a complementary condition. Stabilization in the streamline and crosswind direction is provided by artificial diffusion. The model is completed by including a simple and efficient thermal model and is validated using the numerical values of a reference plain journal bearing experiment under steady-state conditions. In addition, a transient analysis is performed of a journal bearing subjected to a harmonic load. It is shown that the proposed cavitation algorithm results are in good agreement with the reference measurement results. Moreover, the algorithm proves to be stable and requires only a small number of iterations to convergence in the Reynolds-based finite element model.
A Thin C-Band Polarization and Incidence Angle-Insensitive Metamaterial Perfect Absorber
Authors: Fernández Álvarez Humberto , De Cos Gómez Maria , Las-Heras Fernando
Published: April 2015

A novel metamaterial absorber design able to operate in the C frequency band is presented, along with an analysis and a method to improve both its bandwidth and its angular stability. Simulation results for a FR4-based design are shown for comparison with existing designs. In addition, a simplified equivalent circuit is provided for a better understanding of the great angular stability and wide bandwidth exhibited by the proposed structure. Moreover, simulations, manufacturing and measurements of a thinner and more flexible metamaterial absorber, keeping the angular stability of the former one, while providing a wide bandwidth, are presented.
Teflon/SiO2 Bilayer Passivation for Improving the Electrical Reliability of Oxide TFTs Fabricated Using a New Two-Photomask Self-Alignment Process
Authors: Fan Ching-Lin , Shang Ming-Chi , Li Bo-Jyun , Lin Yu-Zuo , Wang Shea-Jue , Lee Win-Der , Hung Bohr-Ran
Published: April 2015

This study proposes a two-photomask process for fabricating amorphous indium–gallium–zinc oxide (a-IGZO) thin-film transistors (TFTs) that exhibit a self-aligned structure. The fabricated TFTs, which lack etching-stop (ES) layers, have undamaged a-IGZO active layers that facilitate superior performance. In addition, we demonstrate a bilayer passivation method that uses a polytetrafluoroethylene (Teflon) and SiO2 combination layer for improving the electrical reliability of the fabricated TFTs. Teflon was deposited as a buffer layer through thermal evaporation. The Teflon layer exhibited favorable compatibility with the underlying IGZO channel layer and effectively protected the a-IGZO TFTs from plasma damage during SiO2 deposition, resulting in a negligible initial performance drop in the a-IGZO TFTs. Compared with passivation-free a-IGZO TFTs, passivated TFTs exhibited superior stability even after 168 h of aging under ambient air at 95% relative humidity.
Inhibition of N-Type Calcium Channels by Fluorophenoxyanilide Derivatives
Authors: Gleeson Ellen , Graham Janease , Spiller Sandro , Vetter Irina , Lewis Richard , Duggan Peter , Tuck Kellie
Published: April 2015

A set of fluorophenoxyanilides, designed to be simplified analogues of previously reported ω-conotoxin GVIA mimetics, were prepared and tested for N-type calcium channel inhibition in a SH-SY5Y neuroblastoma FLIPR assay. N-type or Cav2.2 channel is a validated target for the treatment of refractory chronic pain. Despite being significantly less complex than the originally designed mimetics, up to a seven-fold improvement in activity was observed.
Astaxanthin Attenuates Early Acute Kidney Injury Following Severe Burns in Rats by Ameliorating Oxidative Stress and Mitochondrial-Related Apoptosis
Authors: Guo Song-Xue , Zhou Han-Lei , Huang Chun-Lan , You Chuan-Gang , Fang Quan , Wu Pan , Wang Xin-Gang , Han Chun-Mao
Published: April 2015

Early acute kidney injury (AKI) is a devastating complication in critical burn patients, and it is associated with severe morbidity and mortality. The mechanism of AKI is multifactorial. Astaxanthin (ATX) is a natural compound that is widely distributed in marine organisms; it is a strong antioxidant and exhibits other biological effects that have been well studied in various traumatic injuries and diseases. Hence, we attempted to explore the potential protection of ATX against early post burn AKI and its possible mechanisms of action. The classic severe burn rat model was utilized for the histological and biochemical assessments of the therapeutic value and mechanisms of action of ATX. Upon ATX treatment, renal tubular injury and the levels of serum creatinine and neutrophil gelatinase-associated lipocalin were improved. Furthermore, relief of oxidative stress and tubular apoptosis in rat kidneys post burn was also observed. Additionally, ATX administration increased Akt and Bad phosphorylation and further down-regulated the expression of other downstream pro-apoptotic proteins (cytochrome c and caspase-3/9); these effects were reversed by the PI3K inhibitor LY294002. Moreover, the protective effect of ATX presents a dose-dependent enhancement. The data above suggested that ATX protects against early AKI following severe burns in rats, which was attributed to its ability to ameliorate oxidative stress and inhibit apoptosis by modulating the mitochondrial-apoptotic pathway, regarded as the Akt/Bad/Caspases signalling cascade.
Antifungal Compounds from Cyanobacteria
Authors: Shishido Tânia , Humisto Anu , Jokela Jouni , Liu Liwei , Wahlsten Matti , Tamrakar Anisha , Fewer David , Permi Perttu , Andreote Ana , Fiore Marli , Sivonen Kaarina
Published: April 2015

Cyanobacteria are photosynthetic prokaryotes found in a range of environments. They are infamous for the production of toxins, as well as bioactive compounds, which exhibit anticancer, antimicrobial and protease inhibition activities. Cyanobacteria produce a broad range of antifungals belonging to structural classes, such as peptides, polyketides and alkaloids. Here, we tested cyanobacteria from a wide variety of environments for antifungal activity. The potent antifungal macrolide scytophycin was detected in Anabaena sp. HAN21/1, Anabaena cf. cylindrica PH133, Nostoc sp. HAN11/1 and Scytonema sp. HAN3/2. To our knowledge, this is the first description of Anabaena strains that produce scytophycins. We detected antifungal glycolipopeptide hassallidin production in Anabaena spp. BIR JV1 and HAN7/1 and in Nostoc spp. 6sf Calc and CENA 219. These strains were isolated from brackish and freshwater samples collected in Brazil, the Czech Republic and Finland. In addition, three cyanobacterial strains, Fischerella sp. CENA 298, Scytonema hofmanni PCC 7110 and Nostoc sp. N107.3, produced unidentified antifungal compounds that warrant further characterization. Interestingly, all of the strains shown to produce antifungal compounds in this study belong to Nostocales or Stigonematales cyanobacterial orders.
The Potential of Chitosan and Its Derivatives in Prevention and Treatment of Age-Related Diseases
Authors: Kerch Garry
Published: April 2015

Age-related, diet-related and protein conformational diseases, such as atherosclerosis, diabetes mellitus, cancer, hypercholesterolemia, cardiovascular and neurodegenerative diseases are common in the elderly population. The potential of chitosan, chitooligosaccharides and their derivatives in prevention and treatment of age-related dysfunctions is reviewed and discussed in this paper. The influence of oxidative stress, low density lipoprotein oxidation, increase of tissue stiffness, protein conformational changes, aging-associated chronic inflammation and their pathobiological significance have been considered. The chitosan-based functional food also has been reviewed.
Functional Recombinants Designed from a Fetuin/Asialofetuin-Specific Marine Algal Lectin, Rhodobindin
Authors: Han Jong , Jung Min , Shim Eun , Shim Jun , Kim Young , Kim Gwang
Published: April 2015

Plant lectins have attracted much attention for biomedical applications including targeted drug delivery system and therapy against tumors and microbial infections. The main problem of using lectins as a biomedical tool is a batch-to-batch variation in isoforms content. The production of lectins using recombination tools has the advantage of obtaining high amounts of proteins with more precise properties, but there are only a handful of functional recombinant lectins presently available. A fetuin/asialo-fetuin specific lectin, Rhodobindin, has unique tandem repeats structure which makes it useful in exploiting for recombinant lectin. We developed three functional recombinant lectins using E. coli expression system: one from full cDNA sequence and two from fragmentary sequences of Rhodobindin. Hemagglutinating activity and solubility of the recombinant lectins were highest at OD 0.7 cell concentration at 20 °C. The optimized process developed in this study was suitable for the quality-controlled production of high amounts of soluble recombinant lectins.
Anti-Obesity Activity of the Marine Carotenoid Fucoxanthin
Authors: Gammone Maria , D'Orazio Nicolantonio
Published: April 2015

Nowadays the global tendency towards physical activity reduction and an augmented dietary intake of fats, sugars and calories is leading to a growing propagation of overweight, obesity and lifestyle-related diseases, such diabetes, hypertension, dyslipidemia and metabolic syndrome. In particular, obesity, characterized as a state of low-level inflammation, is a powerful determinant both in the development of insulin resistance and in the progression to type 2 diabetes. A few molecular targets offer hope for anti-obesity therapeutics. One of the keys to success could be the induction of uncoupling protein 1 (UCP1) in abdominal white adipose tissue (WAT) and the regulation of cytokine secretions from both abdominal adipose cells and macrophage cells infiltrated into adipose tissue. Anti-obesity effects of fucoxanthin, a characteristic carotenoid, exactly belonging to xanthophylls, have been reported. Nutrigenomic studies reveal that fucoxanthin induces UCP1 in abdominal WAT mitochondria, leading to the oxidation of fatty acids and heat production in WAT. Fucoxanthin improves insulin resistance and decreases blood glucose levels through the regulation of cytokine secretions from WAT. The key structure of anti-obesity effect is suggested to be the carotenoid end of the polyene chromophore, which contains an allenic bond and two hydroxyl groups. Fucoxanthin, which can be isolated from edible brown seaweeds, recently displayed its many physiological functions and biological properties. We reviewed recent studies and this article aims to explain essential background of fucoxanthin, focusing on its promising potential anti-obesity effects. In this respect, fucoxanthin can be developed into promising marine drugs and nutritional products, in order to become a helpful functional food.
Neuroprotective Effects of the Cultivated Chondrus crispus in a C. elegans Model of Parkinson’s Disease
Authors: Liu Jinghua , Banskota Arjun , Critchley Alan , Hafting Jeff , Prithiviraj Balakrishnan
Published: April 2015

Parkinson’s disease (PD) is the second most common neurodegenerative disorder in the elderly people, currently with no cure. Its mechanisms are not well understood, thus studies targeting cause-directed therapy or prevention are needed. This study uses the transgenic Caenorhabditis elegans PD model. We demonstrated that dietary supplementation of the worms with an extract from the cultivated red seaweed Chondrus crispus decreased the accumulation of α-synulein and protected the worms from the neuronal toxin-, 6-OHDA, induced dopaminergic neurodegeneration. These effects were associated with a corrected slowness of movement. We also showed that the enhancement of oxidative stress tolerance and an up-regulation of the stress response genes, sod-3 and skn-1, may have served as the molecular mechanism for the C. crispus-extract-mediated protection against PD pathology. Altogether, apart from its potential as a functional food, the tested red seaweed, C. crispus, might find promising pharmaceutical applications for the development of potential novel anti-neurodegenerative drugs for humans.
Esters of the Marine-Derived Triterpene Sipholenol A Reverse P-GP-Mediated Drug Resistance
Authors: Zhang Yongchao , Zhang Yun-Kai , Wang Yi-Jun , Vispute Saurabh , Jain Sandeep , Chen Yangmin , Li Jessalyn , Youssef Diaa , Sayed Khalid , Chen Zhe-Sheng
Published: April 2015

Our previous studies showed that several sipholane triterpenes, sipholenol A, sipholenone E, sipholenol L and siphonellinol D, have potent reversal effect for multidrug resistance (MDR) in cancer cells that overexpressed P-glycoprotein (P-gp/ABCB1). Through comparison of cytotoxicity towards sensitive and multi-drug resistant cell lines, we identified that the semisynthetic esters sipholenol A-4-O-acetate and sipholenol A-4-O-isonicotinate potently reversed P-gp-mediated MDR but had no effect on MRP1/ABCC1 and BCRP/ABCG2-mediated MDR. The results from [3H]-paclitaxel accumulation and efflux studies suggested that these two triterpenoids were able to increase the intracellular accumulation of paclitaxel by inhibiting its active efflux. In addition, western blot analysis revealed that these two compounds did not alter the expression levels of P-gp when treated up to 72 h. These sipholenol derivatives also stimulated the ATPase activity of P-gp membranes, which suggested that they might be substrates of P-gp. Moreover, in silico molecular docking studies revealed the virtual binding modes of these two compounds into human homology model of P-gp. In conclusion, sipholenol A-4-O-acetate and sipholenol A-4-O-isonicotinate efficiently inhibit the P-gp and may represent potential reversal agents for the treatment of multidrug resistant cancers.
Piscidin is Highly Active against Carbapenem-Resistant Acinetobacter baumannii and NDM-1-Producing Klebsiella pneumonia in a Systemic Septicaemia Infection Mouse Model
Authors: Pan Chieh-Yu , Chen Jian-Chyi , Chen Te-Li , Wu Jen-Leih , Hui Cho-Fat , Chen Jyh-Yih
Published: April 2015

This study was designed to investigate the antimicrobial activity of two synthetic antimicrobial peptides from an aquatic organism, tilapia piscidin 3 (TP3) and tilapia piscidin 4 (TP4), in vitro and in a murine sepsis model, as compared with ampicillin, tigecycline, and imipenem. Mice were infected with (NDM-1)-producing K. pneumonia and multi-drug resistant Acinetobacter baumannii, and subsequently treated with TP3, TP4, or antibiotics for different periods of time (up to 168 h). Mouse survival and bacterial colony forming units (CFU) in various organs were measured after each treatment. Toxicity was determined based on observation of behavior and measurement of biochemical parameters. TP3 and TP4 exhibited strong activity against K. pneumonia and A. baumannii in vitro. Administration of TP3 (150 μg/mouse) or TP4 (50 μg/mouse) 30 min after infection with K. pneumonia or A. baumannii significantly increased survival in mice. TP4 was more effective than tigecycline at reducing CFU counts in several organs. TP3 and TP4 were shown to be non-toxic, and did not affect mouse behavior. TP3 and TP4 are able at potentiate anti-Acinetobacter baumannii or anti-Klebsiella pneumonia drug activity, reduce bacterial load, and prevent drug resistance, indicating their potential for use in combating multidrug-resistant bacteria.
Egg Phospholipids and Cardiovascular Health
Authors: Blesso Christopher
Published: April 2015

Eggs are a major source of phospholipids (PL) in the Western diet. Dietary PL have emerged as a potential source of bioactive lipids that may have widespread effects on pathways related to inflammation, cholesterol metabolism, and high-density lipoprotein (HDL) function. Based on pre-clinical studies, egg phosphatidylcholine (PC) and sphingomyelin appear to regulate cholesterol absorption and inflammation. In clinical studies, egg PL intake is associated with beneficial changes in biomarkers related to HDL reverse cholesterol transport. Recently, egg PC was shown to be a substrate for the generation of trimethylamine N-oxide (TMAO), a gut microbe-dependent metabolite associated with increased cardiovascular disease (CVD) risk. More research is warranted to examine potential serum TMAO responses with chronic egg ingestion and in different populations, such as diabetics. In this review, the recent basic science, clinical, and epidemiological findings examining egg PL intake and risk of CVD are summarized.
Considering Maternal Dietary Modulators for Epigenetic Regulation and Programming of the Fetal Epigenome
Authors: Chango Abalo , Pogribny Igor
Published: April 2015

Fetal life is characterized by a tremendous plasticity and ability to respond to various environmental and lifestyle factors, including maternal nutrition. Identification of the role of dietary factors that can modulate and reshape the cellular epigenome during development, including methyl group donors (e.g., folate, choline) and bioactive compounds (e.g., polyphenols) is of great importance; however, there is insufficient knowledge of a particular effect of each type of modulator and/or their combination on fetal life. To enhance the quality and safety of food products for proper fetal health and disease prevention in later life, a better understanding of the underlying mechanisms of dietary epigenetic modulators during the critical prenatal period is necessary. This review focuses on the influence of maternal dietary components on DNA methylation, histone modification, and microRNAs, and summarizes current knowledge of the effect and importance of dietary components on epigenetic mechanisms that control the proper expression of genetic information. Evidence reveals that some components in the maternal diet can directly or indirectly affect epigenetic mechanisms. Understanding the underlying mechanisms of how early-life nutritional environment affects the epigenome during development is of great importance for the successful prevention of adult chronic diseases through optimal maternal nutrition.
A Green Algae Mixture of Scenedesmus and Schroederiella Attenuates Obesity-Linked Metabolic Syndrome in Rats
Authors: Kumar Senthil , Magnusson Marie , Ward Leigh , Paul Nicholas , Brown Lindsay
Published: April 2015

This study investigated the responses to a green algae mixture of Scenedesmus dimorphus and Schroederiella apiculata (SC) containing protein (46.1% of dry algae), insoluble fibre (19.6% of dry algae), minerals (3.7% of dry algae) and omega-3 fatty acids (2.8% of dry algae) as a dietary intervention in a high carbohydrate, high fat diet-induced metabolic syndrome model in four groups of male Wistar rats. Two groups were fed with a corn starch diet containing 68% carbohydrates as polysaccharides, while the other two groups were fed a diet high in simple carbohydrates (fructose and sucrose in food, 25% fructose in drinking water, total 68%) and fats (saturated and trans fats from beef tallow, total 24%). High carbohydrate, high fat-fed rats showed visceral obesity with hypertension, insulin resistance, cardiovascular remodelling, and nonalcoholic fatty liver disease. SC supplementation (5% of food) lowered total body and abdominal fat mass, increased lean mass, and attenuated hypertension, impaired glucose and insulin tolerance, endothelial dysfunction, infiltration of inflammatory cells into heart and liver, fibrosis, increased cardiac stiffness, and nonalcoholic fatty liver disease in the high carbohydrate, high fat diet-fed rats. This study suggests that the insoluble fibre or protein in SC helps reverse diet-induced metabolic syndrome.
Countrywide Stereo-Image Matching for Updating Digital Surface Models in the Framework of the Swiss National Forest Inventory
Authors: Ginzler Christian , Hobi Martina
Published: April 2015

Surface models provide key knowledge of the 3-d structure of forests. Aerial stereo imagery acquired during routine mapping campaigns covering the whole of Switzerland (41,285 km2), offers a potential data source to calculate digital surface models (DSMs). We present an automated workflow to generate a nationwide DSM with a resolution of 1 × 1 m based on photogrammetric image matching. A canopy height model (CHM) is derived in combination with an existing digital terrain model (DTM). ADS40/ADS80 summer images from 2007 to 2012 were used for stereo matching, with ground sample distances (GSD) of 0.25 m in lowlands and 0.5 m in high mountain areas. Two different image matching strategies for DSM calculation were applied: one optimized for single features such as trees and for abrupt changes in elevation such as steep rocks, and another optimized for homogeneous areas such as meadows or glaciers. The country was divided into 165,500 blocks, which were matched independently using an automated workflow. The completeness of successfully matched points was high, 97.9%. To test the accuracy of the derived DSM, two reference data sets were used: (1) topographic survey points (n = 198) and (2) stereo measurements (n = 195,784) within the framework of the Swiss National Forest Inventory (NFI), in order to distinguish various land cover types. An overall median accuracy of 0.04 m with a normalized median absolute deviation (NMAD) of 0.32 m was found using the topographic survey points. The agreement between the stereo measurements and the values of the DSM revealed acceptable NMAD values between 1.76 and 3.94 m for forested areas. A good correlation (Pearson’s r = 0.83) was found between terrestrially measured tree height (n = 3109) and the height derived from the CHM. Optimized image matching strategies, an automatic workflow and acceptable computation time mean that the presented approach is suitable for operational usage at the nationwide extent. The CHM will be used to reduce estimation errors of different forest characteristics in the Swiss NFI and has high potential for change detection assessments, since an aerial stereo imagery update is available every six years.
Advancing of Land Surface Temperature Retrieval Using Extreme Learning Machine and Spatio-Temporal Adaptive Data Fusion Algorithm
Authors: Bai Yang , Wong Man , Shi Wen-Zhong , Wu Li-Xin , Qin Kai
Published: April 2015

As a critical variable to characterize the biophysical processes in ecological environment, and as a key indicator in the surface energy balance, evapotranspiration and urban heat islands, Land Surface Temperature (LST) retrieved from Thermal Infra-Red (TIR) images at both high temporal and spatial resolution is in urgent need. However, due to the limitations of the existing satellite sensors, there is no earth observation which can obtain TIR at detailed spatial- and temporal-resolution simultaneously. Thus, several attempts of image fusion by blending the TIR data from high temporal resolution sensor with data from high spatial resolution sensor have been studied. This paper presents a novel data fusion method by integrating image fusion and spatio-temporal fusion techniques, for deriving LST datasets at 30 m spatial resolution from daily MODIS image and Landsat ETM+ images. The Landsat ETM+ TIR data were firstly enhanced based on extreme learning machine (ELM) algorithm using neural network regression model, from 60 m to 30 m resolution. Then, the MODIS LST and enhanced Landsat ETM+ TIR data were fused by Spatio-temporal Adaptive Data Fusion Algorithm for Temperature mapping (SADFAT) in order to derive high resolution synthetic data. The synthetic images were evaluated for both testing and simulated satellite images. The average difference (AD) and absolute average difference (AAD) are smaller than 1.7 K, where the correlation coefficient (CC) and root-mean-square error (RMSE) are 0.755 and 1.824, respectively, showing that the proposed method enhances the spatial resolution of the predicted LST images and preserves the spectral information at the same time.
Bridge Displacement Monitoring Method Based on Laser Projection-Sensing Technology
Authors: Zhao Xuefeng , Liu Hao , Yu Yan , Xu Xiaodong , Hu Weitong , Li Mingchu , Ou Jingping
Published: April 2015

Bridge displacement is the most basic evaluation index of the health status of a bridge structure. The existing measurement methods for bridge displacement basically fail to realize long-term and real-time dynamic monitoring of bridge structures, because of the low degree of automation and the insufficient precision, causing bottlenecks and restriction. To solve this problem, we proposed a bridge displacement monitoring system based on laser projection-sensing technology. First, the laser spot recognition method was studied. Second, the software for the displacement monitoring system was developed. Finally, a series of experiments using this system were conducted, and the results show that such a system has high measurement accuracy and speed. We aim to develop a low-cost, high-accuracy and long-term monitoring method for bridge displacement based on these preliminary efforts.
Noninvasive and Real-Time Plasmon Waveguide Resonance Thermometry
Authors: Zhang Pengfei , Liu Le , He Yonghong , Zhou Yanfei , Ji Yanhong , Ma Hui
Published: April 2015

In this paper, the noninvasive and real-time plasmon waveguide resonance (PWR) thermometry is reported theoretically and demonstrated experimentally. Owing to the enhanced evanescent field and thermal shield effect of its dielectric layer, a PWR thermometer permits accurate temperature sensing and has a wide dynamic range. A temperature measurement sensitivity of 9.4 × 10−3 °C is achieved and the thermo optic coefficient nonlinearity is measured in the experiment. The measurement of water cooling processes distributed in one dimension reveals that a PWR thermometer allows real-time temperature sensing and has potential to be applied for thermal gradient analysis. Apart from this, the PWR thermometer has the advantages of low cost and simple structure, since our transduction scheme can be constructed with conventional optical components and commercial coating techniques.
Development and Beam-Shape Analysis of an Integrated Fiber-Optic Confocal Probe for High-Precision Central Thickness Measurement of Small-Radius Lenses
Authors: Sutapun Boonsong , Somboonkaew Armote , Amarit Ratthasart , Chanhorm Sataporn
Published: April 2015

This work describes a new design of a fiber-optic confocal probe suitable for measuring the central thicknesses of small-radius optical lenses or similar objects. The proposed confocal probe utilizes an integrated camera that functions as a shape-encoded position-sensing device. The confocal signal for thickness measurement and beam-shape data for off-axis measurement can be simultaneously acquired using the proposed probe. Placing the probe’s focal point off-center relative to a sample’s vertex produces a non-circular image at the camera’s image plane that closely resembles an ellipse for small displacements. We were able to precisely position the confocal probe’s focal point relative to the vertex point of a ball lens with a radius of 2.5 mm, with a lateral resolution of 1.2 µm. The reflected beam shape based on partial blocking by an aperture was analyzed and verified experimentally. The proposed confocal probe offers a low-cost, high-precision technique, an alternative to a high-cost three-dimensional surface profiler, for tight quality control of small optical lenses during the manufacturing process.
Measurement Method of Magnetic Field for the Wire Suspended Micro-Pendulum Accelerometer
Authors: Lu Yongle , Li Leilei , Hu Ning , Pan Yingjun , Ren Chunhua
Published: April 2015

Force producer is one of the core components of a Wire Suspended Micro-Pendulum Accelerometer; and the stability of permanent magnet in the force producer determines the consistency of the acceleration sensor’s scale factor. For an assembled accelerometer; direct measurement of magnetic field strength is not a feasible option; as the magnetometer probe cannot be laid inside the micro-space of the sensor. This paper proposed an indirect measurement method of the remnant magnetization of Micro-Pendulum Accelerometer. The measurement is based on the working principle of the accelerometer; using the current output at several different scenarios to resolve the remnant magnetization of the permanent magnet. Iterative Least Squares algorithm was used for the adjustment of the data due to nonlinearity of this problem. The calculated remnant magnetization was 1.035 T. Compared to the true value; the error was less than 0.001 T. The proposed method provides an effective theoretical guidance for measuring the magnetic field of the Wire Suspended Micro-Pendulum Accelerometer; correcting the scale factor and temperature influence coefficients; etc.
Rapid Detection of Ebola Virus with a Reagent-Free, Point-of-Care Biosensor
Authors: Baca Justin , Severns Virginia , Lovato Debbie , Branch Darren , Larson Richard
Published: April 2015

Surface acoustic wave (SAW) sensors can rapidly detect Ebola antigens at the point-of-care without the need for added reagents, sample processing, or specialized personnel. This preliminary study demonstrates SAW biosensor detection of the Ebola virus in a concentration-dependent manner. The detection limit with this methodology is below the average level of viremia detected on the first day of symptoms by PCR. We observe a log-linear sensor response for highly fragmented Ebola viral particles, with a detection limit corresponding to 1.9 × 104 PFU/mL prior to virus inactivation. We predict greatly improved sensitivity for intact, infectious Ebola virus. This point-of-care methodology has the potential to detect Ebola viremia prior to symptom onset, greatly enabling infection control and rapid treatment. This biosensor platform is powered by disposable AA batteries and can be rapidly adapted to detect other emerging diseases in austere conditions.
The Development of Love Wave-Based Humidity Sensors Incorporating Multiple Layers
Authors: Wang Lijun , Liu Jiansheng , He Shitang
Published: April 2015

A Love wave humidity sensor is developed by using a multilayer structure consisting of PVA/SiO2 layers on an ST-90°X quartz substrate. The theoretical result shows that the sensor with such a two-layer structure can achieve a higher sensitivity and a smaller loss than the structures with a single polymer layer. Comparative experiments are performed for the sensor incorporating PVA/SiO2 layers and the sensor incorporating a PVA layer. The experimental results agree well with the theoretical predication.
Exploring Direct 3D Interaction for Full Horizontal Parallax Light Field Displays Using Leap Motion Controller
Authors: Adhikarla Vamsi , Sodnik Jaka , Szolgay Peter , Jakus Grega
Published: April 2015

This paper reports on the design and evaluation of direct 3D gesture interaction with a full horizontal parallax light field display. A light field display defines a visual scene using directional light beams emitted from multiple light sources as if they are emitted from scene points. Each scene point is rendered individually resulting in more realistic and accurate 3D visualization compared to other 3D displaying technologies. We propose an interaction setup combining the visualization of objects within the Field Of View (FOV) of a light field display and their selection through freehand gesture tracked by the Leap Motion Controller. The accuracy and usefulness of the proposed interaction setup was also evaluated in a user study with test subjects. The results of the study revealed high user preference for free hand interaction with light field display as well as relatively low cognitive demand of this technique. Further, our results also revealed some limitations and adjustments of the proposed setup to be addressed in future work.