bluuebunny/crossref_metadata_2025 huggingface dataset:
Bibliometric analysis of vitamin D and obesity research over the period 2000 to 2023
Authors: Song Xudong , Qin Senhua , Chen Shuxin , Zhang Can , Lin Lin , Song Ziyi
Published: July 2024

Background

Globally, the incidence rates of obesity and its related diseases, such as cardiovascular diseases and type 2 diabetes, are continuously rising, posing a significant public health challenge. Studies have indicated a potential correlation between vitamin D deficiency and obesity. However, a quantitative analysis of the studies related vitamin D and obesity is lacking. This investigation aims to fill this gap by providing a comprehensive bibliometric analysis to uncover the collaborative networks, research hotspots, and evolutionary trends within the field of vitamin D and obesity research.

Methods

This study retrieved literature related to vitamin D and obesity from the Web of Science database spanning from 2000 to 2023. Bibliometric analysis was conducted using tools such as HistCite, VOSviewer, and CiteSpace to excavate multi-dimensional information including countries, institutions, authors, journals, citations, and keywords.

Results

A total of 6,144 records were retrieved, involving 123 countries, 6,726 institutions, and 28,156 authors, published in 1,551 journals. The number of published papers and citations showed a generally increasing trend. The United States led in terms of publication volume and influence, with journals such as Nutrients and Obesity Surgery having the highest publication counts. Nasser M. Al-Daghri was the most prolific and influential author. Keyword clustering revealed that research topics covered metabolic health, nutrition, immunity, and bariatric surgery. Citation burst analysis indicated a shift in research focus from the relationship between dietary calcium and obesity to the preventive effects of vitamin D supplementation on metabolic diseases.

Conclusion

The application of bibliometric methods to analyze the research literature in the fields of obesity and vitamin D has provided a comprehensive understanding of the collaborative networks, key research focus, and evolutionary trends in this field, offering insights for guiding future research directions.
Sub-okra leaf shape conferred via chromosomal introgression from Gossypium barbadense L. improves photosynthetic productivity in short-season cotton (Gossypium hirsutum L.)
Authors: Jiang Hui , Ma Xiongfeng , Shi Jialiang , Gao Mingwei , Zhang Xianliang , Zhang Chao , Chai Qichao , Wang Yongcui , Wang Xiuli , Wang Jiabao , Chen Ying , Zhang Donglou , Li Fengrui , Zhao Wenchao , Zhao Junsheng
Published: July 2024

Leaf shape is a vital agronomic trait that affects plant and canopy architecture, yield, and other production attributes of upland cotton. Compared with normal leaves, lobed leaves have potential advantages in improving canopy structure and increasing cotton yield. A chromosomal introgression segment from Gossypium barbadense L. conferring sub-okra leaf shape to Gossypium hirsutum L. was identified on chromosome D01. To determine the effects of this transferred sub-okra leaf shape on the leaf anatomical characteristics, photosynthesis-related traits, and yield of short-season cotton, we performed a field experiment with three sets of near-isogenic lines carrying okra, sub-okra, and normal leaf shape in Lu54 (L54) and Shizao 2 (SZ2) backgrounds. Compared with normal leaves, sub-okra leaves exhibited reduced leaf thickness and smaller leaf mass per area; moreover, the deeper lobes of sub-okra leaves improved the plant canopy structure by decreasing leaf area index by 11.24%–22.84%. Similarly, the intercepted PAR rate of lines with sub-okra leaf shape was also reduced. The chlorophyll content of sub-okra leaves was lower than that of okra and normal leaf shapes; however, the net photosynthetic rate of sub-okra leaves was 8.17%–29.81% higher than that of other leaf shapes at most growth stages. Although the biomass of lines with sub-okra leaf shape was less than that of lines with normal leaves, the average first harvest yield and total yield of lines with the sub-okra leaf shape increased by 6.36% and 5.72%, respectively, compared with those with normal leaves. Thus, improvements in the canopy structure and photosynthetic and physiological characteristics contributed to optimizing the light environment, thereby increasing the yield of lines with sub-okra leaf shape. Our results suggest that the sub-okra leaf trait from G. barbadense L. may have practical applications for cultivating short-season varieties with high photosynthetic efficiency, and improving yield, which will be advantageous for short-season varieties.
Investigating the biosynthesis and roles of the auxin phenylacetic acid during Pseudomonas syringae-Arabidopsis thaliana pathogenesis
Authors: Lee Chia-Yun , Harper Christopher P. , Lee Soon Goo , Qi Yunci , Clay Taylor , Aoi Yuki , Jez Joseph M. , Kasahara Hiroyuki , Blodgett Joshua A. V. , Kunkel Barbara N.
Published: July 2024

Several plant-associated microbes synthesize the auxinic plant growth regulator phenylacetic acid (PAA) in culture; however, the role of PAA in plant-pathogen interactions is not well understood. In this study, we investigated the role of PAA during interactions between the phytopathogenic bacterium Pseudomonas syringae strain PtoDC3000 (PtoDC3000) and the model plant host, Arabidopsis thaliana. Previous work demonstrated that indole-3-acetaldehyde dehydrogenase A (AldA) of PtoDC3000 converts indole-3-acetaldehyde (IAAld) to the auxin indole-3-acetic acid (IAA). Here, we further demonstrated the biochemical versatility of AldA by conducting substrate screening and steady-state kinetic analyses, and showed that AldA can use both IAAld and phenylacetaldehyde as substrates to produce IAA and PAA, respectively. Quantification of auxin in infected plant tissue showed that AldA-dependent synthesis of either IAA or PAA by PtoDC3000 does not contribute significantly to the increase in auxin levels in infected A. thaliana leaves. Using available arogenate dehydratase (adt) mutant lines of A. thaliana compromised for PAA synthesis, we observed that a reduction in PAA-Asp and PAA-Glu is correlated with elevated levels of IAA and increased susceptibility. These results provide evidence that PAA/IAA homeostasis in A. thaliana influences the outcome of plant-microbial interactions.
Gamma-aminobutyric acid treatment promotes resistance against Sogatella furcifera in rice
Authors: Jan Rahmatullah , Asif Saleem , Asaf Sajjad , Lubna  , Khan Zakirullah , Khan Waleed , Kim Kyung-Min
Published: July 2024

The Sogatella furcifera (Horváth) (Homoptera: Delphacidae) is a white-backed planthopper (WBPH) that causes “hopper burn” in rice, resulting in severe yield loss. Gamma-aminobutyric acid (GABA) is a well-known neurotransmitter that inhibits neurotransmission in insects by binding to specific receptors. In this study, we investigated the potential role of GABA in modulating rice resistance to WBPH and evaluated possible defense mechanisms. The experiment was conducted in green house in pots consist of four groups: control, GABA-treated, WBPH-infested, and WBPH-infested treated with GABA. Among the various tested concentration of GABA, 15 mM GABA was applied as a single treatment in water. The treatment was administered one week before WBPH infestation. The results revealed that 15 mM GABA treatment strongly increased WBPH resistance. A plate-based assay indicated that direct application of 15 mM GABA increased the mortality rate of WBPH and increased the damage recovery rate in rice plants. We found that GABA treatment increased the activation of antioxidant enzymes and reduced the reactive oxygen species content and malondialdehyde contents, and reduced the damage rate caused by WBPH. Interestingly, GABA-supplemented plants infested with WBPH exhibited increased phenylalanine ammonia-lyase and pathogenesis-related (PR) genes expression levels. GABA induced the accumulation of abscisic acid (ABA) and salicylic acid (SA) and enhanced the stomata closure and reduced leaf vessels to reduce water conductance during WBPH stress. Furthermore, we found that GABA application to the plant induced the expression of Jasmonic acid (JA) biosynthesis genes (LOX, AOS, AOC, and OPR) and melatonin biosynthesis-related genes (TDC, T5H, ASMT, and SNAT). Our study suggested that GABA increases resistance against WBPH infestation by regulating antioxidant defense system, TCA cycle regulation, phytohormonal signaling, and PR gene regulation.
Distinguishing the role of positivity bias, cognitive impairment and emotional reactivity in the deontological preference in multiple sclerosis during moral dilemmas: a social cognition study protocol
Authors: Zikos Laurent , Degraeve Béatrice , Pinti Antonio , Poupart Julien , Norberciak Laurène , Kwiatkowski Arnaud , Donze Cécile , Lenne Bruno
Published: July 2024

Background

Multiple sclerosis (MS) is a chronic inflammatory and neurodegenerative disease of the central nervous system characterized by a broad and unpredictable range of symptoms, including cognitive and sociocognitive dysfunction. Among these social-cognitive functions, moral judgment has been explored in persons with MS (PwMS) using moral dilemmas, where participants must decide whether to sacrifice one person to save a greater number. Opting for such a sacrifice reflects utilitarian reasoning (sacrificing one for the benefit of many is deemed acceptable), while refusing reflects deontological reasoning (such sacrifice is considered morally wrong). Compared to controls, PwMS have been shown to make greater deontological moral choices in such dilemmas.

Objectives

While PwMS have demonstrated a higher tendency for deontological moral choices in moral dilemmas compared to controls, the underlying determinants of this reasoning pattern remain unclear. In this project, we aim to investigate cognitive, emotional, and motivational factors that may explain deontological decision-making in MS.

Methods and analysis

We will recruit a sample of 45 PwMS and 45 controls aged 18–55 years. The type of response, deontological or utilitarian, to a series of 20 vignettes of moral dilemmas will constitute the primary outcomes. Global cognitive performance, positivity bias, alexithymia and empathy levels as well as emotional reactivity measured by electrodermal activity (EDA) during moral dilemmas will be secondary outcomes.

Ethics and dissemination

Ethics approval was granted by a national ethical committee (CPP Ouest III, national number 2023-A00447-38). The project is sponsored by the ARSEP Foundation. Findings will be presented at national and international conferences, as well as published in peer-reviewed scientific journals.
Impact of life-threatening military incidents during deployments abroad on the relationships between military personnel and their families
Authors: Wesemann Ulrich , Rowlands Katie , Renner Karl-Heinz , Konhäuser Lucas , Köhler Kai , Himmerich Hubertus
Published: July 2024

Introduction

The influence of deployments on family relationships has hardly been investigated. Following a recently proposed new research strategy, military personnel with and without deployment-related life-threatening military incidents during deployment were compared. The hypothesis was that partner and family relationships of military personnel who experienced such an event would deteriorate more.

Methods

This study included N = 255 military personnel who had a romantic partner (n = 78 of them had children) when deployed to Afghanistan. Of these, n = 68 military personnel experienced a deployment-related critical event during the deployment, n = 187 did not. Partnership quality was assessed using a semi-structured pre- and post-deployment interview.

Results

The partner relationships of military personnel who experienced a deployment-related life-threatening military incident during deployment broke up significantly more often. The partner relationships of all military personnel deteriorated significantly, with greater deterioration after deployment in the group who faced such incidents. These results were independent of age, rank or number of previous deployments. In addition, there was a significant deterioration in the relationships between all military personnel and their children with greater deterioration after deployment in the group who faced such incidents.

Conclusion

Life-threatening military incidents during a deployment abroad appear to have a considerable influence on the quality and stability of the partner and family relationships of military personnel. These findings can be used to inform the development of specific pre- and post-deployment measures and training.
Causal attributions of impulsive and compulsive behaviors
Authors: Astudillo-Reyes Karla , Sánchez Ana I. , Luna-Adame María , Martínez María Pilar , Muñoz-López Lucas
Published: July 2024

Introduction

Aggression, and therefore gender-based violence, can be an impulsive or compulsive behavior, depending on the consumption of alcohol and/or drugs. In Europe, the prevalence of gender-based violence is 16 to 23%. This prevalence shows that there is a need to make further progress in the treatment of aggression against women. Qualitative techniques allow us to understand perceptions and attributions holistically by analyzing what people who commit the crime say, why they say it and how they say it.

Aim

To explore the experience of physical and verbal aggression by a partner, dependent on the presence or absence of alcohol and drug use, in the prison population.

Method

A mixed methodology was used (combining qualitative and quantitative techniques). The sample was made up of 140 men divided into two focus groups [with alcohol and/or drug consumption (SAD) and without alcohol and/or drug consumption (NSAD)] who completed the Demographic, Criminal and Behavioral Interview in Penitentiary Institutions; the Gender Violence Questionnaire (both developed for this study) and the MultiCAGE CAD-4 Questionnaire. Qualitative data were analyzed using thematic analysis and quantitative data were obtained using contingency tables.

Results

It was found that the SAD group attributed the crime committed to alcohol and/or drug consumption, while the NSAD group attributed it to jealousy and to their partner. The SAD group revealed that the consequence of the physical aggressions was to get what they were looking for from their partner and the consequences of the verbal aggressions was regret, unlike the NSAD group that did not get anything from the aggressions. The SAD group recognized that to avoid future aggressions they would have to avoid alcohol and/or drug use, while the NSAD group mentioned that they would have to avoid contact with their partner.

Discussion

The need to include perceptions and attributions as well as the use of alcohol and/or drugs is emphasized when assessing individuals who commit the crime of gender-based violence.
Influence parental- and child-related factors on the acceptance of SARS-CoV-2 test methods in schools and daycare facilities
Authors: Loy Johanna K. , Kimmig Christian , Klos Simon , Wunram Heidrun Lioba , Langer Thorsten , Breitinger Eva , Bender Stephan
Published: July 2024

Introduction

Rapid testing for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infections was an essential step in reducing the spread of the virus and monitoring pandemic development. Most mandatory standard pandemic testing in Germany has been performed in schools and daycare facilities. We investigated the influence of behavioral and attitudinal characteristics of children and caregivers on their acceptance of (i) antigen-based nasal swab rapid and (ii) oral saliva-based pooled Polymerase Chain Reaction (PCR) tests.

Methods

Conducted through a cross-sectional survey between November and December 2021, with 1962 caregivers and 581 children/adolescents participating, the study evaluated the acceptability of each testing method on a six-point scale. Participants scored one test method conducted on their child at one of six levels with 1 and 6 denoting “excellent” (1) and “inadequate” (6), respectively. We considered demographic variables, vaccination status, child mental health (measured by the SDQ-questionnaire), and facility type (kindergarten, primary school, secondary school) as covariates.

Results

Results reveal a preference for saliva-based PCR tests over nasal swabs by about one grade, particularly among parents of unvaccinated children, especially if their child expressed future vaccination reluctance. Testing acceptance was lower among children with mental health issues, primary school-aged, and those with less-educated parents. Perception of test accuracy and convenience influenced attitudes, favoring saliva-based PCR tests. Moreover, children with mental health issues felt less secure during testing.

Discussion

To our knowledge, this is the first study to investigate the influence of different testing methods on testing acceptance for SARS-CoV-2 in children and caregivers. Our study identifies predictors of lower acceptance of public health surveillance measures and enables the development of educational programs on testing and vaccination tailored to the needs of specific target groups. Moreover, we demonstrate that test acceptance in vulnerable groups can be enhanced by careful choice of an appropriate testing method.
Investigating seasonal changes in factors associated with COVID-19 concerns: Results from a serial cross-sectional survey study in Germany between 2020 and 2023
Authors: Janzik Robin , Borzekowski Dan , Böl Gaby-Fleur
Published: July 2024

Objective

COVID-19 risk perceptions are discussed to be volatile and have been shown to be connected to the adoption of preventive public health behaviors. This study aimed to investigate changes in COVID-19 concerns and influencing factors as a function of season among the German public.

Methods

Sixty-three waves of cross-sectional telephone surveys with German participants aged 14 years and older conducted at least monthly between June 2020 and April 2023 provided the data basis (N = 63,471). After pooling participants of different waves by season (spring, summer, fall, winter), data were analyzed with regard to changes in physical health, mental health, economic, and social COVID-19 concerns. Individual characteristics (e.g., age), COVID-19 behavior (e.g., hygiene practices), and related perceptions (e.g., controllability of risk) were considered as predictors of composite concerns in different seasons.

Results

Results showed a higher between-seasons than within-seasons variability in concerns, with rises in physical and mental health and social concerns during fall. Multivariate regressions revealed being female, lower education, adopting protective measures, and higher perceived probability of infection in both public and private settings to be consistent predictors of higher COVID-19 concerns. Coefficients of these predictors remained comparatively stable over seasons and years.

Conclusion

Results indicate re-occurring changes in concerns during a prolonged crisis, with distinct characteristics being consistently associated with higher reported concerns. To ensure the application of protective measures, communicators should consider that risk perceptions are subject to fluctuations, but that certain groups of individuals tend to develop them and therefore deserve particular focus.
Practice of key essential nutrition action messages and associated factors among mothers of children aged six months to two years old in Karat town, Konso zone, South Ethiopia, 2024: a community-based cross-sectional study
Authors: Kasse Tsehaynew , Aschalew Zeleke , Desalegn Nathan , Jebero Zenebe , Moga Fikre , Haile Addisalem
Published: July 2024

Background

Key essential nutrition actions (ENA) messages are a comprehensive and evidence-based nutritional package designed to improve the nutritional status during the critical first 1,000 days of life. The poor practice of ENA contributes significantly to mortality and morbidity related to malnutrition in young children. However, there is a dearth of studies focusing on the practice of key ENA messages among mothers and the factors associated with their practice. Therefore, this study aimed to assess the practice of key ENA messages among mothers of children aged 6 months to 2 years in Karat town, Konso zone, South Ethiopia in 2024.

Methods

A community-based cross-sectional study involving 421 mothers of children aged 6 months to 2 years was conducted in Karat town, Konso zone, South Ethiopia from January 15 to February 29, 2024. Respondents were chosen using computer-generated random numbers. A structured, pretested, and interviewer-administered questionnaire was used to collect data. Following coding and entry into EpiData 3.1, the data were exported to SPSS version 25 for analysis. Logistic regression (bivariate and multivariable) was employed to identify factors influencing mothers’ practice of key ENA messages, and statistical significance was set at p < 0.05 with a 95% confidence interval.

Results

The study found that 47.6% (95% CI: 42.8, 52.42%) of mothers demonstrated good practices. Having secondary education or higher, institutional delivery, receiving nutritional counseling during antenatal care (ANC), receipt of postnatal care (PNC) services, having good knowledge, and having a good attitude towards ENA all increase the likelihood of good practice.

Conclusion

This study emphasizes the need for multifaceted interventions to improve ENA practice among mothers residing in Karat town. To effectively address this issue, it is crucial to implement targeted education programs, strengthen postnatal care services, and nutritional counseling into routine antenatal care, promote institutional deliveries, and enhance awareness.
Comprehensive evaluation of global health cities development levels
Authors: Wen Yu , Li Yulan , Zhang Yan , Liu Bingbing
Published: July 2024

Introduction

How to scientifically assess the health status of cities and effectively assist in formulating policies and planning for health city development remains a profound challenge in building a global “health community.”

Methods

This study employs the Building Research Establishment’s International Healthy Cities Index (BRE HCI), encompassing ten environmental categories and fifty-eight indicators, to guide and support the scientific development of healthy cities. The entropy weight-TOPSIS method and the rank sum ratio (RSR) method were applied to comprehensively rank and categorize the health development levels of fifteen global cities. Furthermore, through cluster analysis, this research identifies universal and unique indicators that influence the development of healthy cities.

Results

The results indicate that: (1) Within the scope of 58 evaluation indicators, the precedence in weight allocation is accorded to the kilometres of bicycle paths and lanes per 100,000 population (0.068), succeeded by m2 of public indoor recreation space per capita (0.047), and kilometres of bicycle paths and lanes per 100,000 population (0.042). (2) Among the ten environmental categories, the top three in terms of weight ranking are transport (0.239), leisure and recreation (0.172), and resilience (0.125). Significant disparities exist between different cities and environmental categories, with the issue of uneven health development within cities being particularly prominent. (3) The study categorizes the development levels of healthy cities into three tiers based on composite scores: it classifies Singapore, Shanghai, and Amsterdam at an excellent level; places Dubai and Johannesburg at a comparatively poor level; and situates the remaining ten cities at a moderate level. (4) The analysis identifies 53 international common indicators and 5 characteristic indicators from the 58 indicators based on the significance of the clustering analysis (p < 0.05).

Discussion

The study proposes four strategic recommendations based on these findings: establishing a comprehensive policy assurance system, refining urban spatial planning, expanding avenues for multi-party participation, and augmenting distinctive health indicators. These measures aim to narrow the developmental disparities between cities and contribute to healthy global cities’ balanced and sustainable growth. However, due to existing limitations in sample selection, research methodology application, and the control of potential confounding variables, further in-depth studies are required in the future.
Cost-effectiveness analysis design for interventions to prevent children's oral disease
Authors: Spetz Joanne , Rose Johnie , Kahn James G. , Lin Tracy , Levy Douglas , Pugach Oksana , Hyde Susan , Borrelli Belinda , Henshaw Michelle , Martin Molly , Nelson Suchitra , Ramos-Gomez Francisco , Gansky Stuart A.
Published: July 2024

Introduction

In 2015, the National Institute of Dental and Craniofacial Research (NIDCR) launched the Multidisciplinary Collaborative Research Consortium to Reduce Oral Health Disparities in Children, supporting four randomized trials testing strategies to improve preventive care. A Coordinating Center provides scientific expertise, data acquisition and quality assurance services, safety monitoring, and final analysis-ready datasets. This paper describes the trials' economic analysis strategies, placing these strategies within the broader context of contemporary economic analysis methods.

Methods

The Coordinating Center established a Cost Collaborative Working Group to share information from the four trials about the components of their economic analyses. Study teams indicated data sources for their economic analysis using a set of structured tables. The Group meets regularly to share progress, discuss challenges, and coordinate analytic approaches.

Results

All four trials will calculate incremental cost-effectiveness ratios; two will also conduct cost-utility analyses using proxy diseases to estimate health state utilities. Each trial will consider at least two perspectives. Key process measures include dental services provided to child participants. The non-preference-weighted Early Childhood Oral Health Impact Scale (ECOHIS) will measure oral health-related quality of life. All trials are measuring training, implementation, personnel and supervision, service, supplies, and equipment costs.

Conclusions

Consistent with best practices, all four trials have integrated economic analysis during their planning stages. This effort is critical since poor quality or absence of essential data can limit retrospective analysis. Integrating economic analysis into oral health preventive intervention research can provide guidance to clinicians and practices, payers, and policymakers.
Assessment of advanced neural networks for the dual estimation of water quality indicators and their uncertainties
Authors: Saranathan Arun M. , Werther Mortimer , Balasubramanian Sundarabalan V. , Odermatt Daniel , Pahlevan Nima
Published: July 2024

Given the use of machine learning-based tools for monitoring the Water Quality Indicators (WQIs) over lakes and coastal waters, understanding the properties of such models, including the uncertainties inherent in their predictions is essential. This has led to the development of two probabilistic NN-algorithms: Mixture Density Network (MDN) and Bayesian Neural Network via Monte Carlo Dropout (BNN-MCD). These NNs are complex, featuring thousands of trainable parameters and modifiable hyper-parameters, and have been independently trained and tested. The model uncertainty metric captures the uncertainty present in each prediction based on the properties of the model—namely, the model architecture and the training data distribution. We conduct an analysis of MDN and BNN-MCD under near-identical conditions of model architecture, training, and test sets, etc., to retrieve the concentration of chlorophyll-a pigments (Chl a), total suspended solids (TSS), and the absorption by colored dissolved organic matter at 440 nm (acdom (440)). The spectral resolutions considered correspond to the Hyperspectral Imager for the Coastal Ocean (HICO), PRecursore IperSpettrale della Missione Applicativa (PRISMA), Ocean Colour and Land Imager (OLCI), and MultiSpectral Instrument (MSI). The model performances are tested in terms of both predictive residuals and predictive uncertainty metric quality. We also compared the simultaneous WQI retrievals against a single-parameter retrieval framework (for Chla). Ultimately, the models’ real-world applicability was investigated using a MSI satellite-matchup dataset N=3,053) of Chla and TSS. Experiments show that both models exhibit comparable estimation performance. Specifically, the median symmetric accuracy (MdSA) on the test set for the different parameters in both algorithms range from 30% to 60%. The uncertainty estimates, on the other hand, differ strongly. MDN’s uncertainty estimate is ∼50%, encompassing estimation residuals for 75% of test samples, whereas BNN-MCD’s average uncertainty estimate is ∼25%, encompassing the residuals for 50% of samples. Our analysis also revealed that simultaneous estimation results in improvements in both predictive performance and uncertainty metric quality. Interestingly, the trends mentioned above hold across different sensor resolutions, as well as experimental regimes. This disparity calls for additional research to determine whether such trends in model uncertainty are inherent to specific models or can be more broadly generalized across different algorithms and sensor setups.
Advancements in facial implantology: a review of hydroxyapatite applications and outcomes
Authors: Kauke-Navarro Martin , Knoedler Leonard , Knoedler Samuel , Safi Ali Farid
Published: July 2024

Background

The search for an ideal bone substitute in reconstructive surgery has led to the exploration of various materials, with hydroxyapatite (HaP) emerging as a promising candidate due to its biocompatibility, osteoconductive properties, and structural similarity to human bone. Despite its potential, there is a paucity of data on the long-term safety and efficacy of HaP in facial skeletal reconstruction and augmentation.

Methods

We conducted a systematic review following PRISMA 2020 guidelines, searching PubMed/MEDLINE, Google Scholar, CENTRAL, and Web of Science databases for studies on hydroxyapatite facial implants in reconstruction and augmentation.

Results

Our search yielded 12 studies that met our inclusion criteria, encompassing 74 patients treated with HaP implants for various indications including facial fractures/defects, aesthetic facial balancing, and after tumor resection. The studies reported on outcomes such as implant integration, complications, aesthetic results, and patient satisfaction, with a general trend indicating positive outcomes for the use of HaP in facial reconstruction.

Conclusion

Hydroxyapatite appears to be a viable and effective material for facial skeletal reconstruction and augmentation, offering benefits in terms of biocompatibility, osteoconductivity, and patient outcomes. However, limitations such as low mechanical strength and the need for further research on long-term safety and efficacy were identified. This review underscores the potential of HaP in craniofacial surgery while highlighting areas for future investigation.
Simultaneous detection of infectious bronchitis virus and avian metapneumovirus genotypes A, B, and C by multiplex RT-qPCR assay in chicken tracheal samples in Ecuador
Authors: Loor-Giler Anthony , Muslin Claire , Santander-Parra Silvana , Coello Dayana , De la Torre David , Abad Hernán , Nuñez Luis
Published: July 2024

Respiratory RNA viruses such as Infectious bronchitis virus (IBV) and Avian metapneumovirus (aMPV), which are characterized by generating both respiratory damage and adverse effects on reproductive organs, affect poultry production economically due to high mortality rate and decrease in egg production and quality. Particularly, aMPV has three genotypes that have been reported with greater frequency in chickens: aMPV-A, aMPV-B, and aMPV-C. The present study proposes the design of a multiplex RT-qPCR assay for the simultaneous diagnosis of the 3 genotypes of interest of aMPV and IBV, followed by testing of 200 tracheal samples of vaccinated chickens with respiratory symptoms and finally a phylogenetic analysis of the sequences found. The assay detected up to 1 copy of each viral genome. The standard curves showed an efficiency between 90 and 100% in the multiplex assay and inter- and intra-assay coefficients of variation of 0.363 and 0.459, respectively and inter- and intra-assay coefficients of variation of 0.363 and 0.459, respectively. 69.5% of samples were found positive alone or in coinfection. 114 samples were positive for IBV, 13 for aMPV-A and 25 for aMPV-B. RNA of aMPV-C was no detected. The most commonly found combination was aMPV-B and IBV within 6 samples, and the least common was aMPV-A and aMPV-B in coinfection in 2 samples. The assay was specific for amplification of the genomes of the studied respiratory viruses (IBV, aMPV-A, aMPV-B, aMPV-C) as no amplification was shown from other viral genomes (ChPV, CAstV, ANV, and FAdV) or from the negative controls. Partial genomic Sanger sequencing enabled to identify circulating vaccine-derived and wild-type strains of IBV and vaccine and vaccine-derived strains of aMPV-B. In conclusion, this newly developed multiplex RT-qPCR was shown to be able to detect individual infections as well as co-infections among the respiratory viruses investigated. It was demonstrated to be a reliable and efficient tool for rapidly and safely diagnosing these infections. Furthermore, this study represents the first report of aMPV strains in Ecuadorian poultry and demonstrates the circulation of aMPV-A, aMPV-B, and GI-13 IBV strains in unvaccinated chicken populations in the country. Thus, it highlights the importance of simultaneously identifying these pathogens in greater detail and on a regular basis in Ecuador.
A Temporary Immersion System as a Tool for Lowering Planting Material Production Costs Using the Example of Pennisetum × advena ‘Rubrum’
Authors: Pożoga Mariusz , Olewnicki Dawid , Latocha Piotr
Published: July 2024

The aim of the study was to compare the variable costs of planting material production using the example of vitro cultures of Pennisetum × advena ‘Rubrum’. In the study, temporary immersion system (TIS)- and agar-based methods were used in innovative workday organisation. The workday structure involved a six-hour passaging period followed by a two-hour break for medium preparation, autoclaving, and maintenance tasks. The TIS was found to be more cost-effective than the agar cultures, with lower labour costs and comparable growing expenses. The most expensive element of agar production was labour which was 43% of the costs. The second biggest cost was materials and reagents which represented 25%. In a TIS, production materials and reagents are the most expensive part of production (44%), while labour represents 24% of costs. A TIS offers a much faster multiplication of plants than agar cultures. Plants obtained in the multiplication phase are two times cheaper using a TIS. Rooting accounted for a significant portion of production costs in both methods. Overall, the TIS demonstrated superior efficiency and cost-effectiveness compared to agar cultures in producing Pennisetum × advena ‘Rubrum’ plants.
Variable-Rate Fertilization for Summer Maize Using Combined Proximal Sensing Technology and the Nitrogen Balance Principle
Authors: Zhou Peng , Ou Yazhou , Yang Wei , Gu Yixiang , Kong Yinuo , Zhu Yangxin , Jin Chengqian , Hao Shanshan
Published: July 2024

Soil is a heterogeneous medium that exhibits considerable variability in both spatial and temporal dimensions. Proper management of field variability using variable-rate fertilization (VRF) techniques is essential to maximize crop input–output ratios and resource utilization. Implementing VRF technology on a localized scale is recommended to increase crop yield, decrease input costs, and reduce the negative impact on the surrounding environment. This study assessed the agronomic and environmental viability of implementing VRF during the cultivation of summer maize using an on-the-go detector of soil total nitrogen (STN) to detect STN content in the test fields. A spatial delineation approach was then applied to divide the experimental field into multiple management zones. The amount of fertilizer applied in each zone was determined based on the sensor-detected STN. The analysis of the final yield and economic benefits indicates that plots that adopted VRF treatments attained an average summer maize grain yield of 7275 kg ha−1, outperforming plots that employed uniform-rate fertilization (URF) treatments, which yielded 6713 kg ha−1. Through one-way ANOVA, the yield p values of the two fertilization methods were 6.406 × 10−15, 5.202 × 10−15, 2.497 × 10−15, and 3.199 × 10−15, respectively, indicating that the yield differences between the two fertilization methods were noticeable. This led to an average yield increase of 8.37% ha−1 and a gross profit margin of USD 153 ha−1. In plots in which VRF techniques are utilized, the average nitrogen (N) fertilizer application rate is 627 kg ha−1. In contrast, in plots employing URF methods, the N fertilizer application rate is 750 kg ha−1. The use of N fertilizer was reduced by 16.4%. As a result, there is a reduction in production costs of USD 37.5 ha−1, achieving increased yield while decreasing the amount of applied fertilizer. Moreover, in plots where the VRF method was applied, STN was balanced despite the reduced N application. This observation can be deduced from the variance in summer maize grain yield through various fertilization treatments in a comparative experiment. Future research endeavors should prioritize the resolution of particular constraints by incorporating supplementary soil data, such as phosphorus, potassium, organic matter, and other pertinent variables, to advance and optimize fertilization methodologies.
Machine Learning Methods for Predicting Argania spinosa Crop Yield and Leaf Area Index: A Combined Drought Index Approach from Multisource Remote Sensing Data
Authors: Mouafik Mohamed , Fouad Mounir , El Aboudi Ahmed
Published: July 2024

In this study, we explored the efficacy of random forest algorithms in downscaling CHIRPS (Climate Hazards Group InfraRed Precipitation with Station data) precipitation data to predict Argane stand traits. Nonparametric regression integrated original CHIRPS data with environmental variables, demonstrating enhanced accuracy aligned with ground rain gauge observations after residual correction. Furthermore, we explored the performance of range machine learning algorithms, encompassing XGBoost, GBDT, RF, DT, SVR, LR and ANN, in predicting the Leaf Area Index (LAI) and crop yield of Argane trees using condition index-based drought indices such as PCI, VCI, TCI and ETCI derived from multi-sensor satellites. The results demonstrated the superiority of XGBoost in estimating these parameters, with drought indices used as input. XGBoost-based crop yield achieved a higher R2 value of 0.94 and a lower RMSE of 6.25 kg/ha. Similarly, the XGBoost-based LAI model showed the highest level of accuracy, with an R2 of 0.62 and an RMSE of 0.67. The XGBoost model demonstrated superior performance in predicting the crop yield and LAI estimation of Argania sinosa, followed by GBDT, RF and ANN. Additionally, the study employed the Combined Drought Index (CDI) to monitor agricultural and meteorological drought over two decades, by combining four key parameters, PCI, VCI, TCI and ETCI, validating its accuracy through comparison with other drought indices. CDI exhibited positive correlations with VHI, SPI and crop yield, with a particularly strong and statistically significant correlation observed with VHI (r = 0.83). Therefore, CDI was recommended as an effective method and index for assessing and monitoring drought across Argane forest stands area. The findings demonstrated the potential of advanced machine learning models for improving precipitation data resolution and enhancing agricultural drought monitoring, contributing to better land and hydrological management.
Ecophysiological Recovery of Micropropagated Olive Cultivars: Field Research in an Irrigated Super-High-Density Orchard
Authors: Maldera Francesco , Garofalo Simone Pietro , Camposeo Salvatore
Published: July 2024

This research focuses on the seasonal patterns of the ecophysiological recovery of four olive cultivars (Arbequina, Coratina, Frantoio, and Urano), both micropropagated and self-rooted, grown in a mature, irrigated, super high-density (SHD) orchard under Mediterranean conditions (Southern of Italy). The aim was to observe the impact of the micropropagation method on the ecophysiological responses. Ecophysiological parameters, including leaf water potential (LWP), stomatal conductance (gs), net photosynthetic rate (Pn), and transpiration rate (E) were assessed. Self-rooted trees consistently exhibited superior gs, E, and Pn recovery compared to the micropropagated ones. ‘Arbequina’ maintained elevated levels of Pn under water-deficit conditions. ‘Coratina’ exhibited increases in gs and E after irrigation. ‘Frantoio’ demonstrated recovery capabilities, with lower LWP and higher Pn under stress. ‘Urano’ micropropagated trees achieved higher gs values in mid-summer, while self-rooted trees sustained higher Pn later in the season. This field research highlighted the important role of the propagation method in optimizing the physiological performance of olive cultivars in SHD orchards. Furthermore, it highlighted the necessity of long-term studies on the effects of propagation methods and their interactions with other farming practices.
Serum and Urinary Uromodulin in Dogs with Early Chronic Kidney Disease vs. Healthy Canine Population
Authors: Marečáková Nikola , Kačírová Jana , Tóthová Csilla , Maďari Aladár , Maďar Marián , Kuricová Mária , Horňák Slavomír
Published: July 2024

Serum and urinary uromodulin are evaluated as potential biomarkers of kidney disease. The aim of our research was to select a more appropriate form of uromodulin for the diagnosis of early stages of chronic kidney disease (CKD). We also focused on the influence of age and gender in one breed on uromodulin and on the possible interbreed differences. Serum uromodulin had the lowest values in dogs younger than 2 years but no effect of gender, breed, or CKD was observed. Urinary uromodulin indexed to urinary creatinine was significantly reduced in dogs in stage 2 of CKD (p = 0.003) in contrast to uromodulin converted to urine specific gravity. Urinary uromodulin with both corrections was significantly lower in Belgian shepherds compared to German shepherds (p < 0.0001, p = 0.0054) but was not influenced by gender or age. In stage 1 of CKD, urinary uromodulin correlated with kidney disease markers SDMA (p = 0.0424, p = 0.0214) and UPC (p = 0.0050, p = 0.0024). Urinary uromodulin appears to be more associated with CKD than serum uromodulin. Further studies with a larger number of patients are needed for the suitability of urinary uromodulin as a marker of early-stage disease.
Platelet-Rich Plasma Proteome of Mares Susceptible to Persistent-Breeding-Induced Endometritis Differs from Resistant Mares
Authors: Novello Guilherme , Souza Fabiana F. , Canisso Igor F.
Published: July 2024

Persistent-breeding-induced endometritis (PBIE) is the leading cause of subfertility and poor reproductive efficiency in mares. Platelet-rich plasma (PRP) treatment has been shown to mitigate PBIE, reduce uterine infections, and improve fertility in mares. However, the proteome of PRP in mares, particularly those susceptible to PBIE, remains unknown. This study aimed to fill this knowledge gap by comparing the most abundant proteins present in PRP prepared from mares with histories of being susceptible or resistant to PBIE. The study involved twelve light-breed mares: seven susceptible and five resistant to PBIE. A complete blood count and physical examination were performed on each mare before blood drawing to ensure good health. The PRP was prepared following collection in a blood transfusion bag and double centrifugation. Platelet counts in the PRP were compared across the groups. The PRP was cryopreserved in liquid nitrogen until proteomics could be completed. Physical parameters and complete blood cell counts were within normal ranges. The platelet counts for resistant (561 ± 152 × 103) and susceptible mares (768 ± 395 × 103) differed (p < 0.05). One hundred and five proteins were detected in all mares, and four proteins were more abundant in resistant mares (p < 0.05). The proteins were apolipoprotein C-II, serpin family G member 1, protection of telomeres protein 1, and non-specific serine/threonine protein kinase. All these proteins are linked to the immune response. These results suggest that PRP prepared from mares resistant to PBIE may be more beneficial in mitigating PBIE in mares, offering a promising avenue for improving equine reproductive health. However, this remains to be determined with in vivo studies.
Early Evidence of Post-Mortem Fetal Extrusion in Equids: A Case from the Western Zhou Period (1045–771 BC) Site of Yaoheyuan in Northwestern China
Authors: Huang Zexian , Ma Qiang , Zhang Chengrui , Cheng Ruoxin , Hou Furen , Wu Yi , Luo Feng , Li Yue
Published: July 2024

Post-mortem fetal extrusion, also known as “coffin birth”, refers to the phenomenon where a fetus is pushed out of a deceased female due to pressure from decomposing gas in the abdominal cavity. While post-mortem fetal extrusion has been documented in humans at several archaeological sites, there are few reports of it occurring in non-human animals. In this study, we present a case of post-mortem fetal extrusion in equids observed in a chariot-horse pit (CMK2) at the Western Zhou period site of Yaoheyuan in northwestern China, dating to the early first millennium BC. This specific pit, one of four excavated at the site, contained at least 29 horses and 3 wooden chariots. Most of these horses were young adults aged between 4 and 12 years. Out of the 22 horses with sex estimates, 21 were males. Among these individuals, one adult female horse (Horse 6) and one infantile horse (Horse 10) were of particular importance. Based on the age-at-death, sex, and head orientation of the two individuals, alongside their spatial relationships, it is highly likely that Horse 6 was the fetus of Horse 10 and was extruded in the pit. According to the parturition stage of Horse 10, Horse 6 was likely interred in CMK2 in late spring or early summer of the year, during which the relatively high temperature may have generated gas that led to the extrusion of the fetus. Although the specific reason for the inclusion of a pregnant mare in a chariot-horse pit at Yaoheyuan remains a topic for future research, this case marks the first report of post-mortem fetal extrusion in archaeological horses. The findings offer insights into the timing of horse interment as part of ritual practices among the settled elites during the Bronze Age in China and provide valuable reference data for contemporary equine veterinary science.