bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Shear Stress Waves on Meat Tenderness: Ultrasonoporation
Authors: Reyes-Villagrana Raúl Alberto , Madrigal-Melchor Jesús , Chávez-Martínez América , Juárez-Moya Juliana , Rentería-Monterrubio Ana Luis
Published: June 2023

Meat is an important part of the food pyramid in Mexico, to such an extent that it is included in the basic food basket. In recent years, there has been great interest in the application of so-called emerging technologies, such as high-intensity ultrasound (HIU), to modify the characteristics of meat and meat products. The advantages of the HIU in meat such as pH, increased water-holding capacity, and antimicrobial activity are well documented and conclusive. However, in terms of meat tenderization, the results are confusing and contradictory, mainly when they focus on three HIU parameters: acoustic intensity, frequency, and application time. This study explores via a texturometer the effect of HIU-generated acoustic cavitation and ultrasonoporation in beef (m. Longissimus dorsi). Loin-steak was ultrasonicated with the following parameters: time tHIU = 30 min/each side; frequency fHIU = 37 kHz; acoustic intensity IHIU = ~6, 7, 16, 28, and 90 W/cm2. The results showed that acoustic cavitation has a chaotic effect on the loin-steak surface and thickness of the rib-eye due to Bjerknes force, generating shear stress waves, and acoustic radiation transmittance via the internal structure of the meat and the modification of the myofibrils, in addition to the collateral effect in which the collagen and pH generated ultrasonoporation. This means that HIU can be beneficial for the tenderization of meat.
Protein Quality and Protein Digestibility of Vegetable Creams Reformulated with Microalgae Inclusion
Authors: Prandi Barbara , Boukid Fatma , Van De Walle Simon , Cutroneo Sara , Comaposada Josep , Van Royen Geert , Sforza Stefano , Tedeschi Tullia , Castellari Massimo
Published: June 2023

Microalgae are considered a valuable source of proteins that are used to enhance the nutritional value of foods. In this study, a standard vegetable cream recipe was reformulated through the addition of single-cell ingredients from Arthrospira platensis (spirulina), Chlorella vulgaris, Tetraselmis chui, or Nannochloropsis oceanica at two levels of addition (1.5% and 3.0%). The impact of microalgae species and an addition level on the amino acid profile and protein in vitro digestibility of the vegetable creams was investigated. The addition of microalgae to vegetable creams improved the protein content and the amino acid nutritional profile of vegetable creams, whereas no significant differences were observed in protein digestibility, regardless of the species and level of addition, indicating a similar degree of protein digestibility in microalgae species despite differences in their protein content and amino acid profile. This study indicates that the incorporation of microalgae is a feasible strategy to increase the protein content and nutritional quality of foods.
A Genotyping Method for Detecting Foreign Buffalo Material in Mozzarella di Bufala Campana Cheese Using Allele-Specific- and Single-Tube Heminested-Polymerase Chain Reaction
Authors: Rullo Rosario , Caira Simonetta , Nicolae Ioana , Marino Francesca , Addeo Francesco , Scaloni Andrea
Published: June 2023

Mozzarella di Bufala Campana (MdBC) cheese is a Protected Designation of Origin (PDO) product that is important for the economy and cultural heritage of the Campania region. Food fraud can undermine consumers’ trust in this dairy product and harm the livelihood of local producers. The current methods for detecting adulteration in MdBC cheese due to the use of buffalo material from foreign countries could exhibit limitations associated with the required use of expensive equipment, time-consuming procedures, and specialized personnel. To address these limits here, we propose a rapid, reliable, and cost-effective genotyping method that can detect foreign buffalo milk in a counterpart from the PDO area and in MdBC cheese, ensuring the quality and authenticity of the latter dairy product. This method is based on dedicated allele-specific and single-tube heminested polymerase chain reaction procedures. By using allele-specific primers that are designed to detect the nucleotide g.472G>C mutation of the CSN1S1Bbt allele, we distinguished an amplicon of 330 bp in the amplification product of DNA when extracted from milk and cheese, which is specific to the material originating from foreign countries. By spiking foreign milk samples with known amounts of the counterpart from the PDO area, the sensitivity of this assay was determined to be 0.01% v/v foreign to PDO milk. Based on a rough estimate of its simplicity, reliability, and cost, this method could be a valuable tool for identifying adulterated buffalo PDO dairy products.
Asymptotics for Time-Fractional Venttsel’ Problems in Fractal Domains
Authors: Capitanelli Raffaela , Creo Simone , Lancia Maria Rosaria
Published: June 2023

In this study, we consider fractional-in-time Venttsel’ problems in fractal domains of the Koch type. Well-posedness and regularity results are given. In view of numerical approximation, we consider the associated approximating pre-fractal problems. Our main result is the convergence of the solutions of such problems towards the solution of the fractional-in-time Venttsel’ problem in the corresponding fractal domain. This is achieved via the convergence (in the Mosco–Kuwae–Shioya sense) of the approximating energy forms in varying Hilbert spaces.
Scale-Invariant General Fractional Calculus: Mellin Convolution Operators
Authors: Tarasov Vasily E.
Published: June 2023

General fractional calculus (GFC) of operators that is defined through the Mellin convolution instead of Laplace convolution is proposed. This calculus of Mellin convolution operators can be considered as an analogue of the Luchko GFC for the Laplace convolution operators. The proposed general fractional differential operators are generalizations of scaling (dilation) differential operator for the case of general form of nonlocality. Semi-group and scale-invariant properties of these operators are proven. The Hadamard and Hadamard-type fractional operators are special case of the proposed operators. The fundamental theorems for the scale-invariant general fractional operators are proven. The proposed GFC can be applied in the study of dynamics, which is characterized by nonlocality and scale invariance.
Dense Molecular Environments of B[e] Supergiants and Yellow Hypergiants
Authors: Kraus Michaela , Kourniotis Michalis , Arias María Laura , Torres Andrea F. , Nickeler Dieter H.
Published: June 2023

Massive stars expel large amounts of mass during their late evolutionary phases. We aim to unveil the physical conditions within the warm molecular environments of B[e] supergiants (B[e]SGs) and yellow hypergiants (YHGs), which are known to be embedded in circumstellar shells and disks. We present K-band spectra of two B[e]SGs from the Large Magellanic Cloud and four Galactic YHGs. The CO band emission detected from the B[e]SGs LHA 120-S 12 and LHA 120-S 134 suggests that these stars are surrounded by stable rotating molecular rings. The spectra of the YHGs display a rather diverse appearance. The objects 6 Cas and V509 Cas lack any molecular features. The star [FMR2006] 15 displays blue-shifted CO bands in emission, which might be explained by a possible close to pole-on oriented bipolar outflow. In contrast, HD 179821 shows blue-shifted CO bands in absorption. While the star itself is too hot to form molecules in its outer atmosphere, we propose that it might have experienced a recent outburst. We speculate that we currently can only see the approaching part of the expelled matter because the star itself might still block the receding parts of a (possibly) expanding gas shell.
Hollow Particles Obtained by Prilling and Supercritical Drying as a Potential Conformable Dressing for Chronic Wounds
Authors: Sellitto Maria Rosaria , Amante Chiara , Aquino Rita Patrizia , Russo Paola , Rodríguez-Dorado Rosalía , Neagu Monica , García-González Carlos A. , Adami Renata , Del Gaudio Pasquale
Published: June 2023

The production of aerogels for different applications has been widely known, but the use of polysaccharide-based aerogels for pharmaceutical applications, specifically as drug carriers for wound healing, is being recently explored. The main focus of this work is the production and characterization of drug-loaded aerogel capsules through prilling in tandem with supercritical extraction. In particular, drug-loaded particles were produced by a recently developed inverse gelation method through prilling in a coaxial configuration. Particles were loaded with ketoprofen lysinate, which was used as a model drug. The core-shell particles manufactured by prilling were subjected to a supercritical drying process with CO2 that led to capsules formed by a wide hollow cavity and a tunable thin aerogel layer (40 μm) made of alginate, which presented good textural properties in terms of porosity (89.9% and 95.3%) and a surface area up to 417.0 m2/g. Such properties allowed the hollow aerogel particles to absorb a high amount of wound fluid moving very quickly (less than 30 s) into a conformable hydrogel in the wound cavity, prolonging drug release (till 72 h) due to the in situ formed hydrogel that acted as a barrier to drug diffusion.
A Validation Study of a Deep Learning-Based Doping Drug Text Recognition System to Ensure Safe Drug Use among Athletes
Authors: Lee Sang-Yong , Park Jae-Hyeon , Yoon Jiwun , Lee Ji-Yong
Published: June 2023

This study aimed to develop an English version of a doping drug-recognition system using deep learning-based optical character recognition (OCR) technology. A database of 336 banned substances was built based on the World Anti-Doping Agency’s International Standard Prohibited List and the Korean Pharmaceutical Information Center’s Drug Substance Information. For accuracy and validity analysis, 886 drug substance images, including 152 images of prescriptions and drug substance labels collected using data augmentation, were used. The developed hybrid system, based on the Tesseract OCR model, can be accessed by both a smartphone and website. A total of 5379 words were extracted, and the system showed character recognition errors regarding 91 words, showing high accuracy (98.3%). The system correctly classified all 624 images for acceptable substances, 218 images for banned substances, and incorrectly recognized 44 of the banned substances as acceptable. The validity analysis showed a high level of accuracy (0.95), sensitivity (1.00), and specificity (0.93), suggesting system validity. The system has the potential of allowing athletes who lack knowledge about doping to quickly and accurately check whether they are taking banned substances. It may also serve as an efficient option to support the development of a fair and healthy sports culture.
Urinary Incontinence Affects the Quality of Life and Increases Psychological Distress and Low Self-Esteem
Authors: AlQuaiz AlJohara M. , Kazi Ambreen , AlYousefi Nada , Alwatban Lemmese , AlHabib Yara , Turkistani Iqbal
Published: June 2023

Urinary incontinence is a common problem among women of reproductive age. The objectives of this study were to measure the prevalence of urinary incontinence and the association with quality of life, psychological distress and self-esteem in Saudi women in the city of Riyadh. A questionnaire-based cross-sectional study was conducted in primary healthcare centers with Saudi women aged between 30 and 75 years. The questionnaire consisted of Urinary Distress Inventory, Incontinence Impact Questionnaire, Kessler Psychological Distress Scale, Rosenberg Self-esteem Scale, and the Female Sexual Function Index. Around 47.5% of women were suffering from urinary incontinence. The most common type of incontinence was stress (79%), followed by urge (72%) and mixed type (51%). Multivariate logistic regression analysis found that stress (5.83 (3.1, 11.1)), urge (3.41 (2.0, 5.8)), mixed (8.71 (3.4, 22.4)) incontinence and severe urinary distress (8.11 (5.2, 12.7)) were associated with impaired quality of life. Women suffering from stress and urge incontinence were twice (2.0 (1.3, 2.2)) as likely of reporting moderate/severe mental distress. Women suffering from urge incontinence (1.92 (1.4, 2.7)) and severe urinary distress (1.74 (1.1, 2.8)) were at a higher prevalence of reporting low self-esteem. Urinary incontinence affects the physical, psychological, social, and sexual health of women. Healthcare providers should be knowledgeable about the adverse consequences of UI on women’s personal and social life, and provide counseling and treatment accordingly.
A Qualitative Investigation of Staff Feedback on an Online Learning Module on Smoking Cessation in a German Healthcare Company
Authors: Vitzthum Karin , Cerci Deniz
Published: June 2023

Quitting smoking is a powerful way for patients to improve their own wellbeing and to significantly reduce the risk of health problems. Evidence shows that health professionals can effectively intervene in order to prevent and stop tobacco smoking in their patients. Online learning modules have proved to be effective in terms of transferring knowledge and skills. In an urban community hospital setting in Germany, a novel e-learning course for staff on the treatment of tobacco dependence was implemented in 2021. In this study, we analyzed free-text feedback of participants completing this online module in order to examine the feasibility and acceptance of this new format. We were able to reach a reasonable proportion of staff. Our qualitative analysis showed that most feedback was positive and described the module as well-designed and helpful. Some staff, however, expressed extremely negative views and did not see smoking cessation support as essential to their role in healthcare. We argue that in order to achieve a shift in attitude in healthcare staff, a change in German policy is required which includes the creation of smoke-free environments and the adherence to smoke-free policies on hospital sites. Furthermore, the provision of smoking cessation support in line with the WHO Framework Convention on Tobacco Control and a true understanding of the role of all healthcare professionals in promoting health of patients and staff will be essential.
Applying Machine Learning to Healthcare Operations Management: CNN-Based Model for Malaria Diagnosis
Authors: Cho Young Sik , Hong Paul C.
Published: June 2023

The purpose of this study is to explore how machine learning technologies can improve healthcare operations management. A machine learning-based model to solve a specific medical problem is developed to achieve this research purpose. Specifically, this study presents an AI solution for malaria infection diagnosis by applying the CNN (convolutional neural network) algorithm. Based on malaria microscopy image data from the NIH National Library of Medicine, a total of 24,958 images were used for deep learning training, and 2600 images were selected for final testing of the proposed diagnostic architecture. The empirical results indicate that the CNN diagnostic model correctly classified most malaria-infected and non-infected cases with minimal misclassification, with performance metrics of precision (0.97), recall (0.99), and f1-score (0.98) for uninfected cells, and precision (0.99), recall (0.97), and f1-score (0.98) for parasite cells. The CNN diagnostic solution rapidly processed a large number of cases with a high reliable accuracy of 97.81%. The performance of this CNN model was further validated through the k-fold cross-validation test. These results suggest the advantage of machine learning-based diagnostic methods over conventional manual diagnostic methods in improving healthcare operational capabilities in terms of diagnostic quality, processing costs, lead time, and productivity. In addition, a machine learning diagnosis system is more likely to enhance the financial profitability of healthcare operations by reducing the risk of unnecessary medical disputes related to diagnostic errors. As an extension for future research, propositions with a research framework are presented to examine the impacts of machine learning on healthcare operations management for safety and quality of life in global communities.
Effects of Magnetic Field and Ultrasound Applications on Endogenous Melatonin Content and Drought Stress Tolerance of Pepper Seedlings
Authors: Yakupoğlu Gökçen
Published: June 2023

Uncertainty about climate change exposes agriculture to high risks. Magnetic field (MF) applications are one of the methods that can be used to reduce the effects of environmental stress conditions. Melatonin (MEL) acts as a broad-spectrum antioxidant in eliminating the effects of damage caused in plants exposed to stress. This study aims to examine the effect of MF and ultrasound (US) applications on endogenous MEL levels in peppers and investigate the usability of treated seeds against drought stress. Pre-sowing pepper seeds, 0, 0.3, 0.9, 1.1 T MF and 0, 15, 30 min 40 Hz US were applied. The results show that the highest increase in MEL content was detected at 0.3 T MF with 82% and with 30 min of US application at 98%. MF and US treatments decreased the malondialdehyde (MDA) (19% and 35%, respectively) and hydrogen peroxide (H2O2) (52% and 58%, respectively) contents in seedlings. An increase of 24% and 22% (MF-US, respectively) was observed in catalase (CAT) enzyme activity with these applications. As a result, it was observed that MF and US treatments increased the endogenous MEL content and provided tolerance against drought stress. MF and US applications could be suggested as methods to increase drought tolerance in peppers by increasing the endogenous MEL content.
A Simple Method for Drip Irrigation Scheduling of Spinach (Spinacia oleracea L.) in a Plastic Greenhouse in the North China Plain Using a 20 Cm Standard Pan Outside the Greenhouse
Authors: Niu Junfang , Feng Junxia , Liu Shengyao , Jia Songnan , Fan Fengcui
Published: June 2023

The objective of this paper is to perform drip irrigation scheduling for spinach (Spinacia oleracea L.) in a plastic greenhouse using the standard 20 cm evaporation pan. A drip irrigation experiment with four levels of irrigation, i.e., 0.6, 0.8, 1.0, and 1.2 times the cumulative evaporation of a 20 cm standard pan (Epan) were set up. The irrigation interval was controlled using a 20 mm Epan, and optimal irrigation water amounts of 0.8 Epan and 0.6 Epan were recommended for the spring and autumn growing seasons, respectively. Overirrigation (1.2 Epan) also led to yield losses, particularly for stem growth. In addition, a proper greenhouse index, defined as the ratio of the cumulative Epan inside and outside the greenhouse, could predict the Epan inside the greenhouse using the external Epan to a high degree of accuracy (daily data with r2 = 0.85, root mean square error (RMSE = 0.68 mm d−1), for a 4-day interval with r2 = 0.95, RMSE = 1.81 mm 4 day−1, and for the entire growth period with r2 = 1.0, RMSE = 2.40 mm). A simple and low-cost greenhouse index method could be used to formulate drip irrigation schedules for spinach in low-technology plastic greenhouses using a 20 cm standard pan outside the greenhouse.
Integration of Chitosan and Biopesticides to Suppress Pre-Harvest Diseases of Apple
Authors: DeGenring Liza , Peter Kari , Poleatewich Anissa
Published: June 2023

The natural product chitosan has been shown to reduce plant disease severity and enhance the efficacy of microbial biocontrol agents in several crops. However, little is known about the potential synergisms between chitosan and biopesticides and best use practices in apple production. The objectives of this study were to evaluate the effect of pre-harvest applications of chitosan alone and in combination with a commercial biopesticide to suppress fungal diseases of apple and to investigate the potential for chitosan to reduce the quantity of overwintering Venturia inaequalis spores in orchard leaf litter. Chitosan products, Tidal Grow and ARMOUR-Zen 15, and a commercial biopesticide, Serenade ASO, were tested in a research orchard in Pennsylvania and commercial orchards in New Hampshire. Chitosan applications reduced apple scab incidence and severity by up to 55% on fruit compared to the water control. Chitosan also reduced sooty blotch, flyspeck, and rust incidence on fruit. Furthermore, a chitosan + biopesticide treatment overlayed onto a grower standard spray program reduced diseases more effectively than the grower standard alone. However, this efficacy was dependent on the cultivar and pathogen. Chitosan did not reduce overwintering V. inaequalis ascospores. This research provides evidence that pre-harvest chitosan applications have the potential for disease management in apple production.
Genome-Wide Identification, Characterization, and Expression Pattern of MYB Gene Family in Melastoma candidum
Authors: Li Hui , Wen Xiaoxia , Wei Mingke , Huang Xiong , Dai Seping , Ruan Lin , Yu Yixun
Published: June 2023

The MYB gene family is significant in plants, playing a role in numerous plant development processes, including metabolism, hormone signal transduction, cell identity, and biotic and abiotic stresses. Due to the recent availability of the Melastoma candidum genome, this is the first time that the MYB gene family has been identified in this species. This study identified 421 MYB gene members in the M. candidum genome using the HMMER search and BLASTp method. These MYBs were further divided into 10 sub-types, including R2R3, R-R, CPC-like, CCA1-like, TBP-like, R1R2R3, I-box, atypical MYB, MYB-CC, and MYB-like. Domain and conservation analyses revealed that each type of MYB was characterized by a different number and combination of SANTs/myb DNA-binding domains. Collinearity analysis revealed several gene duplication events within the MYB gene family. The Ka to Ks ratio suggested that most of the MYB genes underwent purifying selection during the evolution process. Phylogenetic analysis among three species confirmed our findings and displayed the evolutionary relationship of MYB genes in different species. RNA-seq of three developmental stages of flowers and WGCNA analysis identified McMYB113h, McMYB21b, and McGLK1c as playing a pivotal role during flower development in M. candidum. Finally, we conducted qRT-PCR experiments for 20 flower-development-related MYBs across 9 tissues to illustrate their expression patterns in M. candidum. This study establishes a foundation for exploring MYB gene resources and their potential applications in related industries of M. candidum.
Improvement in Physiobiochemical and Yield Characteristics of Pea Plants with Nano Silica and Melatonin under Salinity Stress Conditions
Authors: Al-Shammari Wasimah B. , Altamimi Haya R. , Abdelaal Khaled
Published: June 2023

The effect of nano silica (50 mL L−1) and melatonin (75 µM) individually or in combination in foliar applications on the morphophysiological, biochemical and yield properties of pea plants under salinity stress conditions was evaluated. Salt stress caused a remarkable decrease in the growth and yield characteristics; for example, the plant dry weight, plant height, number of flowers plant−1, number of pods plant−1, weight of 100 green seeds and protein concentration in the pea plants during both seasons were decreased compared with the control. Similarly, their physiobiochemical characteristics were negatively affected; chlorophyll a, chlorophyll b and the relative water content (RWC) were significantly reduced in the stressed pea plants. However, malondialdehyde (MDA), hydrogen peroxide, the electrolyte leakage (EL%), super oxide and the antioxidant components (catalase (CAT), superoxide dismutase (SOD), peroxidase (POX) and total phenolic compounds) were significantly increased when the plants were under salt stress compared with the control plants. On the other hand, the foliar application of nano silica and melatonin individually or in combination enhanced the physiobiochemical characteristics, morphological characteristics and yield of the stressed pea plants. The best treatment was the combination treatment (nano silica + melatonin), which caused significant increases in the plant dry weight, plant height, number of flowers and pods plant−1, weight of 100 green seeds, protein concentration, chlorophyll concentrations and RWC in the stressed pea plants. Additionally, the combination treatment significantly decreased the EL%, MDA, O2⋅− and H2O2 and adjusted the upregulation of the antioxidant enzymes, proline and total phenolic compounds in the stressed plants compared with the stressed untreated pea plants. Generally, it can be suggested that the co-application of nano silica (50 mL L−1) + melatonin (75 µM) plays a positive role in alleviating the adverse impacts of salinity on pea plants by modifying the plant metabolism and regulating the antioxidant defense system as well as scavenging reactive oxygen species.
Ternary Ni-Ce-Mg-O Composites: In-Depth Optical Spectroscopy Study and Catalytic Performance in CO Oxidation
Authors: Veselov Grigory B. , Stoyanovskii Vladimir O. , Vedyagin Aleksey A.
Published: June 2023

In the present work, ternary Ni-Ce-Mg-O composites containing various amounts of NiO and CeO2 were synthesized via a sol-gel approach. Aqueous solutions of cerium and nickel nitrates were introduced at the stage of hydrolysis of magnesium methoxide, which allowed for avoiding the use of expensive organic precursors. It was revealed that the properties of the composites were defined by the complex interactions between NiO, CeO2, and MgO components. In order to perform an in-depth characterization of the prepared samples, diffuse reflectance UV–vis and Raman spectroscopies were applied. According to the results of these methods, Mg2+ ions did not substitute Ce4+ ions in the CeO2 lattice. However, in the case of the Ni-containing samples, approximately 2–3% of the Ce4+ ions were substituted by Ni2+, thus resulting in the formation of vacancies in the CeO2. The strong interaction of NiO with MgO predictably resulted in the formation of NixMg1−xO solid solutions. When the NiO content in the sample was 20 wt%, the composition of the formed solid solution was estimated to be Ni0.60Mg0.40O. In addition, the presence of CeO2 affected the texture of the ternary composites, thus leading to a slight decrease in the specific surface area. The catalytic performance of the Ni-Ce-Mg-O composites was examined in the CO oxidation reaction under prompt thermal aging conditions. The choice of reaction conditions was due to a high sensitivity of the CO oxidation response toward the available metal surface area and possible metal-support interactions.
Barriers to Physical Activity for Women with Physical Disabilities: A Systematic Review
Authors: Olasagasti-Ibargoien Jurgi , Castañeda-Babarro Arkaitz , León-Guereño Patxi , Uria-Olaizola Naroa
Published: June 2023

Physical activity is essential for women with physical disabilities. This review aims to identify the barriers they face in practicing sport. A systematic review was conducted using the PubMed/Medline, Scopus, and Web of Science databases in January 2023, with an update in March 2023. The eligibility criteria used for inclusion were as follows. (i) Women with physical disabilities; (ii) women who engage in or want to engage in physical activities and/or sport, both adapted and non-adapted; (iii) identification of women’s barriers to such practice; (iv) research articles; and (v) papers written in English and published in peer-reviewed journals. The exclusion were as follows. (i) Women with illness, injury or transient physical activity difficulties; (ii) mention of rehabilitative physical activity; and (iii) results showing no differentiation in barrier types by gender. This review identified different barriers, grouped into eight types according to the differentiating factor, thus showing that disable people’s participation in physical activity is directly related to some specific barriers which seem to differ according to their gender. Therefore, the success of participation in physical activities depends not only on the user’s concern, but also on an inclusive social environment.
A Computational Approach to Hand Pose Recognition in Early Modern Paintings
Authors: Bernasconi Valentine , Cetinić Eva , Impett Leonardo
Published: June 2023

Hands represent an important aspect of pictorial narration but have rarely been addressed as an object of study in art history and digital humanities. Although hand gestures play a significant role in conveying emotions, narratives, and cultural symbolism in the context of visual art, a comprehensive terminology for the classification of depicted hand poses is still lacking. In this article, we present the process of creating a new annotated dataset of pictorial hand poses. The dataset is based on a collection of European early modern paintings, from which hands are extracted using human pose estimation (HPE) methods. The hand images are then manually annotated based on art historical categorization schemes. From this categorization, we introduce a new classification task and perform a series of experiments using different types of features, including our newly introduced 2D hand keypoint features, as well as existing neural network-based features. This classification task represents a new and complex challenge due to the subtle and contextually dependent differences between depicted hands. The presented computational approach to hand pose recognition in paintings represents an initial attempt to tackle this challenge, which could potentially advance the use of HPE methods on paintings, as well as foster new research on the understanding of hand gestures in art.
Live Cell Light Sheet Imaging with Low- and High-Spatial-Coherence Detection Approaches Reveals Spatiotemporal Aspects of Neuronal Signaling
Authors: Potcoava Mariana , Contini Donatella , Zurawski Zachary , Huynh Spencer , Mann Christopher , Art Jonathan , Alford Simon
Published: June 2023

Light sheet microscopy in live cells requires minimal excitation intensity and resolves three-dimensional (3D) information rapidly. Lattice light sheet microscopy (LLSM) works similarly but uses a lattice configuration of Bessel beams to generate a flatter, diffraction-limited z-axis sheet suitable for investigating subcellular compartments, with better tissue penetration. We developed a LLSM method for investigating cellular properties of tissue in situ. Neural structures provide an important target. Neurons are complex 3D structures, and signaling between cells and subcellular structures requires high resolution imaging. We developed an LLSM configuration based on the Janelia Research Campus design or in situ recording that allows simultaneous electrophysiological recording. We give examples of using LLSM to assess synaptic function in situ. In presynapses, evoked Ca2+ entry causes vesicle fusion and neurotransmitter release. We demonstrate the use of LLSM to measure stimulus-evoked localized presynaptic Ca2+ entry and track synaptic vesicle recycling. We also demonstrate the resolution of postsynaptic Ca2+ signaling in single synapses. A challenge in 3D imaging is the need to move the emission objective to maintain focus. We have developed an incoherent holographic lattice light-sheet (IHLLS) technique to replace the LLS tube lens with a dual diffractive lens to obtain 3D images of spatially incoherent light diffracted from an object as incoherent holograms. The 3D structure is reproduced within the scanned volume without moving the emission objective. This eliminates mechanical artifacts and improves temporal resolution. We focus on LLS and IHLLS applications and data obtained in neuroscience and emphasize increases in temporal and spatial resolution using these approaches.
Correction: Capasso et al. Regular Wave Seakeeping Analysis of a Planing Hull by Smoothed Particle Hydrodynamics: A Comprehensive Validation. J. Mar. Sci. Eng. 2023, 11, 700
Authors: Capasso Salvatore , Tagliafierro Bonaventura , Mancini Simone , Martínez-Estévez Iván , Altomare Corrado , Domínguez José M. , Viccione Giacomo
Published: June 2023

In the original publication [...]
Sedimentological, Diagenetic, and Sequence Stratigraphic Controls on the Shallow to Marginal Marine Carbonates of the Middle Jurassic Samana Suk Formation, North Pakistan
Authors: Qamar Shazia , Shah Mumtaz Muhammad , Janjuhah Hammad Tariq , Kontakiotis George , Shahzad Amir , Besiou Evangelia
Published: June 2023

This study presents a thorough analysis of the sedimentology, diagenesis, and sequence stratigraphy of the Middle Jurassic Samana Suk Formation in the Hazara Basin of northern Pakistan. Focusing on two sections, namely Mera Rehmat and Por Miana, the research aims to unravel the complex geological processes within the formation. The examination of microfacies reveals nine distinct depositional textures, ranging from mudstone to wackestone, packstone, and grainstone, indicating various inner ramp environments such as open marine, lagoon, and coastal settings. Petrographic investigations shed light on diagenetic processes, including micritization, cementation, dissolution, compaction, neomorphism, and dolomitization. Six cementation types are identified, and the dolomitization patterns vary, providing insights into lagoonal environments and mudstone replacement. Sequence stratigraphic analysis uncovers intriguing patterns within the Samana Suk Formation. The high-stand system tract is characterized by mudstones, pelloidal grainstones, and dolomitized mudstones, indicating periods of high sea level. In contrast, the transgressive system tract displays ooidal grainstones, pelloidal packstones, and pel-bioclastic grainstones, representing transgression and inundation of previously exposed areas. A significant finding is the impact of diagenesis on reservoir quality parameters, specifically porosity, and permeability. Diagenetic processes, cementation types, and dolomitization patterns have significantly altered the pore network, highlighting the importance of considering diagenesis in assessing the Samana Suk Formation as a hydrocarbon reservoir. This research provides a comprehensive understanding of the sedimentology, diagenesis, and sequence stratigraphy of the Middle Jurassic Samana Suk Formation. The findings contribute to our knowledge of similar carbonate reservoirs globally, enhancing the exploration and development of hydrocarbon resources in comparable depositional environments.
Analytical and Computational Modeling for Multi-Degree of Freedom Systems: Estimating the Likelihood of an FOWT Structural Failure
Authors: Gaidai Oleg , Xu Jingxiang , Yakimov Vladimir , Wang Fang
Published: June 2023

Wind turbines and their associated parts are subjected to cyclical loads, such as bending, torque, longitudinal stresses, and twisting moments. The novel spatiotemporal reliability technique described in this research is especially useful for high-dimensional structural systems that are either measured or numerically simulated during representative observational time span. As this study demonstrates, it is possible to predict risks of dynamic system failure or damage given the in situ environmental load pattern. As an engineering example for this reliability, the authors have chosen 10-MW floating wind turbines and their dynamic responses, under environmental loadings, caused by wind and waves. The aim of this study was to benchmark a state-of-the-art approach suitable for the reliable study of offshore wind turbines. Existing reliability methods do not easily cope with dynamic system high dimensionality. The advocated reliability technique enables accurate and efficient assessment of dynamic system failure probability, accounting for system nonlinearities and high dimensionality as well as cross-correlations between different system components.
Locally Isolated Trichoderma harzianum Species Have Broad Spectrum Biocontrol Activities against the Wood Rot Fungal Species through Both Volatile Inhibition and Mycoparasitism
Authors: Chan Mu En , Tan Jhing Yein , Lee Yan Yi , Lee Daryl , Fong Yok King , Mutwil Marek , Wong Jia Yih , Hong Yan
Published: June 2023

Pathogenic root/wood rot fungal species infect multiple urban tree species in Singapore. There is a need for sustainable and environmentally friendly mitigation. We report the local Trichoderma strains as potential biocontrol agents (BCAs) for pathogenic wood rot fungal species such as Phellinus noxius, Rigidoporus microporus, and Fulvifomes siamensis. Isolated Trichoderma strains were DNA-barcoded for their molecular identities and assessed for their potential as a BCA by their rate of growth in culture and effectiveness in inhibiting the pathogenic fungi in in vitro dual culture assays. Trichoderma harzianum strain CE92 was the most effective in inhibiting the growth of the pathogenic fungi tested. Preliminary results suggested both volatile organic compound (VOC) production and direct hyphal contact contributed to inhibition. SPME GC-MS identified known fungal inhibitory volatiles. Trichoderma harzianum strain CE92 hyphae were found to coil around Phellinus noxius and Lasiodiplodia theobromae upon contact in vitro and were possibly a part of the mycoparasitism. In summary, the work provides insight into Trichoderma inhibition of pathogenic fungi and identifies local strains with good potential for broad-spectrum BCAs against root/wood rot fungi in Singapore.
Molecular Phylogeny and Morphology Reveal Cryptic Species in the Cordyceps militaris Complex from Vietnam
Authors: Wang Yao , Dong Quan-Ying , Luo Run , Fan Qi , Duan Dong-E , Dao Van-Minh , Wang Yuan-Bing , Yu Hong
Published: June 2023

The Cordyceps militaris complex, which is a special group in the genus Cordyceps, is rich in species diversity and is widely distributed in nature. Throughout the investigations of arthropod-pathogenic fungi in the national reserves and in Vietnam parks, collections of C. militaris attacking lepidopteran pupae or larvae were located in the soil and on the leaf litter. The phylogenetic analyses of the combined nrSSU, nrLSU, TEF, RPB1, and RPB2 sequence data indicated that the fungal materials collected in Vietnam belonged to C. militaris and two hidden species in the C. militaris complex. The phylogenetic analyses and morphological comparisons presented here strongly supported the descriptions of C. polystromata and C. sapaensis as new taxa as well as C. militaris as a known species. The morphological characteristics of 11 species in the C. militaris complex, which included two novel species and nine known taxa, were also compared.
The Impacts of Shopping Tourism on Retail Sales and Rents: Lessons from the COVID-19 Quasi-Experiment of Hong Kong
Authors: Yiu Chung Yim
Published: June 2023

This research studies the impact of shopping tourism on retail sales and rents, using the COVID-19 pandemic as a quasi-experiment. Shopping tourism refers to individuals who travel primarily for shopping purposes, and their spending patterns can have significant effects on the retail sector. The COVID-19 pandemic disrupted global travel and resulted in a decline in shopping tourist arrivals, leading to a downturn in sales for retailers dependent on shopping tourism. Additionally, the decline in shopping tourism affected retail rents, as the reduced demand for retail spaces posed challenges for property owners in attracting tenants. The study focuses on Hong Kong, a prominent shopping destination, which experienced a significant decline in tourist arrivals and retail sales during the pandemic. The research analyses the relationship between tourist arrivals, retail sales, and rents using time series analysis and identifies the impact of shopping tourism on retail rents. The findings suggest a positive association between tourist arrivals and retail sales and rents, particularly during the period of shopping tourism growth. However, the pandemic severely reduced this effect, revealing the impact of shopping tourists on the retail sector. The study concludes by discussing the implications for retail resilience and highlighting the need for further research on the impacts of shopping tourism on retail sales and rents.
Input Flux and the Risk of Heavy Metal(Loid) of Agricultural Soil in China: Based on Spatiotemporal Heterogeneity from 2000 to 2021
Authors: Ma Wenyu , Pan Yuchun , Sun Zaijin , Liu Changhua , Li Xiaolan , Xu Li , Gao Yunbing
Published: June 2023

Identifying the current status of the heavy metal(loid) input of agricultural soils is vital for the soil ecological environment of agricultural-producing areas. Most previous studies have typically carried been out in small regions with limited sampling sites, which is insufficient to reveal the overall status of China. This study reviewed publications from over the past 20 years and calculated the input fluxes of heavy metal(loid)s in agricultural soil via atmospheric deposition, fertilizer, manure, and irrigation in different regions of China based on spatiotemporal heterogeneity using a meta-analysis, providing more accurate and reliable results. It was found that the heavy metal(loid) input flux of atmospheric deposition in China is large, while that of fertilizer and manure is relatively low compared to Europe. The major sources of As, Cd, Cr, Ni, and Pb entering the soil was atmospheric deposition, which accounted for 12% to 92% of the total input. Manure was responsible for 19% to 75% of the Cu and Zn input. Cd is the element presenting the most significant risk to the environment of agricultural soils in China and its safety limit will be reached within 100 years for most regions. The region we need to be concerned about is Huang-Huai-Hai due to its comprehensive pollution.
Bioabsorbable Polymeric Stent for the Treatment of Coarctation of the Aorta (CoA) in Children: A Methodology to Evaluate the Design and Mechanical Properties of PLA Polymer
Authors: dos Santos Flávio José , Hernandez Bruno Agostinho , Santos Rosana , Machado Marcel , Souza Mateus , Capello Sousa Edson A. , Andrade Aron
Published: June 2023

This study presents a methodology that combines experimental tests and the finite element method, which is able to analyse the influence of the geometry on the mechanical behaviour of stents made of bioabsorbable polymer PLA (PolyLactic Acid) during their expansion in the treatment of coarctation of the aorta (CoA). Tensile tests with standardized specimen samples were conducted to determine the properties of a 3D-printed PLA. A finite element model of a new stent prototype was generated from CAD files. A rigid cylinder simulating the expansion balloon was also created to simulate the stent opening performance. A tensile test with 3D-printed customized stent specimens was performed to validate the FE stent model. Stent performance was evaluated in terms of elastic return, recoil, and stress levels. The 3D-printed PLA presented an elastic modulus of 1.5 GPa and a yield strength of 30.6 MPa, lower than non-3D-printed PLA. It can also be inferred that crimping had little effect on stent circular recoil performance, as the difference between the two scenarios was on average 1.81%. For an expansion of diameters ranging from 12 mm to 15 mm, as the maximum opening diameter increases, the recoil levels decrease, ranging from 10 to 16.75% within the reported range. These results point out the importance of testing the 3D-printed PLA under the conditions of using it to access its material properties; the results also indicate that the crimping process could be disregarded in simulations to obtain fast results with lower computational cost and that new proposed stent geometry made of PLA might be suitable for use in CoA treatments—the approach that has not been applied before. The next steps will be to simulate the opening of an aorta vessel using this geometry.
Low-Temperature Fabrication of Plate-like α-Al2O3 with Less NH4F Additive
Authors: Chen Haiyang , Li Bin , Liu Meng , Yang Xue , Liu Jie , Qin Tingwei , Xue Zejian , Xing Yun , Chen Junhong
Published: June 2023

Fluorinated compounds are effective mineralization agents for the fabrication of plate-like α-Al2O3. However, in the preparation of plate-like α-Al2O3, it is still an extremely challenging task to reduce the content of fluoride while ensuring a low synthesis temperature. Herein, oxalic acid and NH4F are proposed for the first time as additives in the preparation of plate-like α-Al2O3. The results showed that plate-like α-Al2O3 can be synthesized at a low temperature of 850 °C with the synergistic effect of oxalic acid and 1 wt.% NH4F. Additionally, the synergistic effect of oxalic acid and NH4F not only can reduce the conversion temperature of α-Al2O3 but also can change the phase transition sequence.
Enhanced Hardness-Toughness Balance Induced by Adaptive Adjustment of the Matrix Microstructure in In Situ Composites
Authors: Zhao Mingjuan , Jiang Xiang , Guan Yumeng , Yang Haichao , Zhao Longzhi , Liu Dejia , Jiao Haitao , Yu Meng , Tang Yanchuan , Zhang Laichang
Published: June 2023

With the development of high-speed and heavy-haul railway transportation, the surface failure of rail turnouts has become increasingly severe due to insufficient high hardness-toughness combination. In this work, in situ bainite steel matrix composites with WC primary reinforcement were fabricated via direct laser deposition (DLD). With the increased primary reinforcement content, the adaptive adjustments of the matrix microstructure and in situ reinforcement were obtained at the same time. Furthermore, the dependence of the adaptive adjustment of the composite microstructure on the composites’ balance of hardness and impact toughness was evaluated. During DLD, the laser induces an interaction among the primary composite powders, which leads to obvious changes in the phase composition and morphology of the composites. With the increased WC primary reinforcement content, the dominant sheaves of the lath-like bainite and the few island-like retained austenite are changed into needle-like lower bainite and plenty of block-like retained austenite in the matrix, and the final reinforcement of Fe3W3C and WC is obtained. In addition, with the increased primary reinforcement content, the microhardness of the bainite steel matrix composites increases remarkably, but the impact toughness decreases. However, compared with conventional metal matrix composites, the in situ bainite steel matrix composites manufactured via DLD possess a much better hardness-toughness balance, which can be attributed to the adaptive adjustment of the matrix microstructure. This work provides a new insight into obtaining new materials with a good combination of hardness and toughness.
The Friction of Radially Loaded Hybrid Spindle Bearings under High Speeds
Authors: Gärtner Marcus , Brecher Christian , Neus Stephan , Eckel Hans-Martin , Bartelt Andreas , Hoppert Maik , Ilkhani Mohammad Reza
Published: June 2023

Friction losses are an important parameter for evaluating the operational behaviour of high-speed rolling bearings. Specifically, in machine tool applications, the bearings are subjected to high radial loads and high speeds, which lead to increased forces in the rolling contact and, as a result, increased bearing friction. In this high-speed application, hybrid spindle bearings, typically made of ceramic balls and steel raceways, show better frictional behaviour compared to full steel-made bearings. Therefore, precise knowledge of the friction characteristics of high-speed hybrid bearings can improve friction models and generalise them to spindle bearings with different types, geometries, and operating conditions. In this article, a new straightforward and cost-efficient method for measuring the frictional torque in spindle bearings is presented. A rigidly arranged 7008 hybrid spindle bearing pair was tested up to rotational speeds of 24,000 rpm and high radial loads of 3 kN. The effects of oil–air and grease lubrication are discussed in characteristic diagrams of the tested bearings. Then, based on the test results, a friction calculation model is presented and validated for the outer race control and minimised power dissipation regarding the influence of radial forces.