bluuebunny/crossref_metadata_2025 huggingface dataset:
Contactless Video-Based Heart Rate Monitoring of a Resting and an Anesthetized Pig
Authors: Wang Meiqing , Youssef Ali , Larsen Mona , Rault Jean-Loup , Berckmans Daniel , Marchant-Forde Jeremy N. , Hartung Joerg , Bleich André , Lu Mingzhou , Norton Tomas
Published: February 2021

Heart rate (HR) is a vital bio-signal that is relatively easy to monitor with contact sensors and is related to a living organism’s state of health, stress and well-being. The objective of this study was to develop an algorithm to extract HR (in beats per minute) of an anesthetized and a resting pig from raw video data as a first step towards continuous monitoring of health and welfare of pigs. Data were obtained from two experiments, wherein the pigs were video recorded whilst wearing an electrocardiography (ECG) monitoring system as gold standard (GS). In order to develop the algorithm, this study used a bandpass filter to remove noise. Then, a short-time Fourier transform (STFT) method was tested by evaluating different window sizes and window functions to accurately identify the HR. The resulting algorithm was first tested on videos of an anesthetized pig that maintained a relatively constant HR. The GS HR measurements for the anesthetized pig had a mean value of 71.76 bpm and standard deviation (SD) of 3.57 bpm. The developed algorithm had 2.33 bpm in mean absolute error (MAE), 3.09 bpm in root mean square error (RMSE) and 67% in HR estimation error below 3.5 bpm (PE3.5). The sensitivity of the algorithm was then tested on the video of a non-anaesthetized resting pig, as an animal in this state has more fluctuations in HR than an anaesthetized pig, while motion artefacts are still minimized due to resting. The GS HR measurements for the resting pig had a mean value of 161.43 bpm and SD of 10.11 bpm. The video-extracted HR showed a performance of 4.69 bpm in MAE, 6.43 bpm in RMSE and 57% in PE3.5. The results showed that HR monitoring using only the green channel of the video signal was better than using three color channels, which reduces computing complexity. By comparing different regions of interest (ROI), the region around the abdomen was found physiologically better than the face and front leg parts. In summary, the developed algorithm based on video data has potential to be used for contactless HR measurement and may be applied on resting pigs for real-time monitoring of their health and welfare status, which is of significant interest for veterinarians and farmers.
Supplementation Effect of Oleuropein Extract Combined with Betaine, Magnesium, and Vitamin E on Pigs’ Performance and Meat Quality Characteristics
Authors: Rey Ana I. , Puig Patricia , Cardozo Paul William , Hechavarría Teresa
Published: February 2021

This study evaluates the effect of the dietary combination of oleuropein extract (1200 mg/kg) and betaine (1000 mg/kg), magnesium oxide (600 mg/kg), and α–tocopheryl acetate (400 mg/kg), or a half-dose of these compounds, on pigs’ performance, oxidative status, and meat quality characteristics (drip loss, TBARS, and texture and fatty acid profile of intramuscular fat). Sixty-six barrows and females were slaughtered at 120 kg of BW. Performance and carcass yield were not changed by treatments. The high-dose mixture resulted in higher serum ferric reducing/antioxidant power (p = 0.0026), lower glucose (p = 0.03) and a tendency to have lower serum TBARS (p = 0.07) when compared to control. Percentage of drip loss, moisture content, intramuscular fat, or texture parameters were not modified by dietary treatments. Pigs supplemented with the high-dose mixture had higher PUFA (p = 0.0001), n-6 (p = 0.0001), n-3 (p = 0.0095) and lower MUFA (p = 0.0064) in the neutral lipid fraction of intramuscular fat. Free PUFA, mainly n-3 fatty acids (p = 0.0009), were also higher in the meat of pigs fed the high-dose mixture compared with the others. A higher mobilization (neutral to free fatty acids hydrolysis) of n-3 and MUFA fatty acids in the muscle from pigs fed the high-dose mixture was observed. However, dietary mixture supplementation tended to increase MUFA (p = 0.056) and decrease the total PUFA (p = 0.0074) proportions in muscle polar lipids. This specific fatty acid composition of meat from pigs supplemented with the high-dose mixture could be responsible for the higher meat lipid oxidation observed in this group when compared to the other groups. Consequently, the low-dose mixture would be more adequate for maintaining the oxidative status of pigs and, meat lipid stability.
Congenital Malformations in Sea Turtles: Puzzling Interplay between Genes and Environment
Authors: Martín-del-Campo Rodolfo , Calderón-Campuzano María Fernanda , Rojas-Lleonart Isaías , Briseño-Dueñas Raquel , García-Gasca Alejandra
Published: February 2021

The completion of embryonic development depends, in part, on the interplay between genetic factors and environmental conditions, and any alteration during development may affect embryonic genetic and epigenetic regulatory pathways leading to congenital malformations, which are mostly incompatible with life. Oviparous reptiles, such as sea turtles, that produce numerous eggs in a clutch that is buried on the beach provide an opportunity to study embryonic mortality associated with malformations that occur at different times during development, or that prevent the hatchling from emerging from the nest. In sea turtles, the presence of congenital malformations frequently leads to mortality. A few years ago, a detailed study was performed on external congenital malformations in three species of sea turtles from the Mexican Pacific and Caribbean coasts, the hawksbill turtle, Eretmochelys imbricata (n = 23,559 eggs), the green turtle, Chelonia mydas (n = 17,690 eggs), and the olive ridley, Lepidochelys olivacea (n = 20,257 eggs), finding 63 types of congenital malformations, of which 38 were new reports. Of the three species, the olive ridley showed a higher incidence of severe anomalies in the craniofacial region (49%), indicating alterations of early developmental pathways; however, several malformations were also observed in the body, including defects in the carapace (45%) and limbs (33%), as well as pigmentation disorders (20%), indicating that deviations occurred during the middle and later stages of development. Although intrinsic factors (i.e., genetic mutations or epigenetic modifications) are difficult to monitor in the field, some environmental factors (such as the incubation temperature, humidity, and probably the status of feeding areas) are, to some extent, less difficult to monitor and/or control. In this review, we describe the aetiology of different malformations observed in sea turtle embryos, and provide some actions that can reduce embryonic mortality.
Boswellia serrata Resin Extract in Diets of Nile Tilapia, Oreochromis niloticus: Effects on the Growth, Health, Immune Response, and Disease Resistance to Staphylococcus aureus
Authors: Montaser Metwally M. , El-sharnouby Mohamed E. , EL-Noubi Gamal , El-Shaer Heba M. , Khalil Alshimaa A. , Hassanin Mohamed , Amer Shimaa A. , El-Araby Doaa A.
Published: February 2021

The influences of Boswellia serrata resin extract (BSRE) as a feed additive on the growth performance, immune response, antioxidant status, and disease resistance of Nile tilapia, Oreochromis niloticus L. were assessed. One hundred-forty four fingerlings (initial weight: 21.82 ± 0.48 g) were randomly allotted into four groups with three replicates where they were fed on one of four treatments with four levels of Boswellia serrata resin extract 0, 5, 10, or 15 g kg−1, BSRE0, BSRE5, BSRE10, BSRE15, respectively for eight weeks. After the end of the feeding trial, the fish were challenged with Staphylococcus aureus, and mortalities were noted. The final body weight, total body weight gain, and the total feed intake were quadratically increased in BSRE5 treatment (p < 0.01). The protein productive efficiency (PPE) was linearly and quadratically increased in all BSRE supplemented treatments (p < 0.01). Dietary addition of BSRE raised the fish crude protein content and reduced the fat content in a level-dependent manner (p < 0.01). The ash content was raised in the BSRE15 group (p < 0.01). Dietary BSRE supplementation decreased the serum levels of glucose, total cholesterol, triglycerides, and nitric oxide. It increased the serum levels of total protein, albumin, total globulins, α1 globulin, α2 globulin, ß globulin, ɣ globulin, Catalase, and SOD (superoxide dismutase) activity, GSH (reduced glutathione), lysozyme activity, and MPO (myeloperoxidase) in a level-dependent manner (p < 0.05). The BSRE15 diet increased the serum level of ALT (alanine aminotransferase) and decreased creatinine serum level (p < 0.05). Dietary BSRE supplementation increased the relative percentage of survival % (RPS) of S. aureus challenged fish. The histoarchitecture of the gills and kidney was normal in the BSRE5 treatment and moderately changed in BSRE10 and BSRE15 treatments. The splenic lymphoid elements were more prevalent, and the melano-macrophage centers (MMC) were mild to somewhat activated in BSRE supplemented treatments. Dietary BSRE supplementation improved the intestinal histomorphology. It can be concluded that BSRE addition can enhance the antioxidant activity, immune status, and disease resistance of O. niloticus to S. aureus infection. The level of 5 g kg−1 BSRE can improve fish growth without causing harmful effects on fish health. The highest levels of BSRE are not recommended as they badly affected the histoarchitecture of many vital organs.
Distinct Pattern of NPY in Gastro–Entero–Pancreatic System of Goat Kids Fed with a New Standardized Red Orange and Lemon Extract (RLE)
Authors: De Felice Elena , Giaquinto Daniela , Damiano Sara , Salzano Angela , Fabroni Simona , Ciarcia Roberto , Scocco Paola , de Girolamo Paolo , D’Angelo Livia
Published: February 2021

The use of natural compounds as feed additive is also increasing in farm animals, thanks to the beneficial effect on both animals and consumers health. Here, we questioned whether natural extracts, such as red orange and lemon extract (RLE) rich in flavanones, anthocyanins, and other polyphenols, used as feed additives could display an effect on the neuropeptide Y (NPY) in the gastro–entero–pancreatic tract of goat kids. NPY is one of the most abundant neuropeptides in mammals, known for its orexigenic role although it is involved in many central and peripheral functions. We carried out immunohistochemical analyses on samples of abomasum, duodenum and pancreas collected from two experimental groups: one fed with standard diet and one with standard diet + RLE. For the first time we document NPY distribution in the abomasum, duodenum and pancreas of goats and observe the highest number of NPY positive cells in neuroendocrine cells of duodenum. Remarkably, upon RLE feed supplementation, NPY immunoreactive cells increased significantly in abomasal epithelium and pancreatic islets but not in duodenum, likely due to pH variation of abomasum and duodenum. Our observations represent a baseline for future studies on the interaction between neuropeptides and polyphenols, used as feed additive.
Myoglobin Concentration and Oxygen Stores in Different Functional Muscle Groups from Three Small Cetacean Species
Authors: Arregui Marina , Singleton Emily M. , Saavedra Pedro , Pabst D. Ann , Moore Michael J. , Sierra Eva , Rivero Miguel A. , Câmara Nakita , Niemeyer Misty , Fahlman Andreas , McLellan William A. , Bernaldo de Quirós Yara
Published: February 2021

Compared with terrestrial mammals, marine mammals possess increased muscle myoglobin concentrations (Mb concentration, g Mb · 100g−1 muscle), enhancing their onboard oxygen (O2) stores and their aerobic dive limit. Although myoglobin is not homogeneously distributed, cetacean muscle O2 stores have been often determined by measuring Mb concentration from a single muscle sample (longissimus dorsi) and multiplying that value by the animal’s locomotor muscle or total muscle mass. This study serves to determine the accuracy of previous cetacean muscle O2 stores calculations. For that, body muscles from three delphinid species: Delphinus delphis, Stenella coeruleoalba, and Stenella frontalis, were dissected and weighed. Mb concentration was calculated from six muscles/muscle groups (epaxial, hypaxial and rectus abdominis; mastohumeralis; sternohyoideus; and dorsal scalenus), each representative of different functional groups (locomotion powering swimming, pectoral fin movement, feeding and respiration, respectively). Results demonstrated that the Mb concentration was heterogeneously distributed, being significantly higher in locomotor muscles. Locomotor muscles were the major contributors to total muscle O2 stores (mean 92.8%) due to their high Mb concentration and large muscle masses. Compared to this method, previous studies assuming homogenous Mb concentration distribution likely underestimated total muscle O2 stores by 10% when only considering locomotor muscles and overestimated them by 13% when total muscle mass was considered.
Fast Focus Ultrasound Liver Technique for the Assessment of Cystic Echinococcosis in Sheep
Authors: Borriello Giuliano , Guccione Jacopo , Di Loria Antonio , Bosco Antonio , Pepe Paola , Prisco Francesco , Cringoli Giuseppe , Paciello Orlando , Rinaldi Laura , Ciaramella Paolo
Published: February 2021

A complete ultrasound examination (cUS) of the liver was performed on 172 female sheep and compared to the performance of a fast-focused ultrasound technique to diagnose echinococcal cysts. The scanned area was divided in: HYP (right hypocondrium), zone (Z)1 from HYP to the 11th intercostal space (IS), Z2 (10th–8th IS) and Z3 (7th–5th IS). Contiguous scans were also examined (HYP + Z1, Z1 + Z2, Z2 + Z3). Furthermore, during the procedures, the sheep were divided into three groups according to the body weight: Group (G) 1 (lighter), G2 (medium), and G3 (heavier). Finally, diagnostic outcomes were compared with necropsy findings. cUS obtained the highest values of sensitivity (Se) (91%), Specificity (Sp) (80%), and positive-zones (124/138, 90%), as compared to the other scans. cUS was also characterized by high values of Se and Sp and was able to identify a great number of positive-zones, when sheep were divided by body-weight groups. Similar performances were obtained in G1 by HYP (Se: 91%–Sp: 82%; 18/20, 90% of positive-zones) and HYP + Z1 scans (Se: 91%–Sp: 82; 90% of positive-zones, 18/20). Thus, in lighter breeds, the examination of HYP and HYP + Z1 scan windows could be considered reliable techniques for identifying the infected animals, while in heavier sheep the cUS still represents the best option.
Relationships between the Rider’s Pelvic Mobility and Balance on a Gymnastic Ball with Equestrian Skills and Effects on Horse Welfare
Authors: Uldahl Mette , Christensen Janne W. , Clayton Hilary M.
Published: February 2021

Riders need core stability to follow and guide the horse’s movements and avoid giving unintended or conflicting signals. This study evaluated the rider’s performance of exercises on a gymnastic ball with on-horse performance and indicators of stress in the horse. Twenty experienced riders were scored performing three exercises on a gymnastic ball and for quality and harmony when riding based on evaluation of video recordings in which conflict behaviours were evident. The horse’s heart rate and number of conflict behaviors during the riding test and cortisol levels after completion of the test were measured. The rider’s ability to roll the pelvis from side-to-side on a gymnastic ball was highly correlated with ability to circle the pelvis on the ball and with quality and harmony during riding. However, pelvic roll and riding quality and harmony showed a trend toward a negative correlation with balancing skills on the ball. It appears that the ability to actively move the pelvis is more relevant to equestrian performance than static balancing skill. Horses ridden by riders with better pelvic mobility and control showed significantly fewer conflict behaviors. On the contrary, high scores for balancing on the gymnastic ball were negatively correlated with the horses’ working heart rates, suggesting a less energetic performance. Pelvic control and mobility may be predictive for equestrian skills and riding harmony.
From the Horse’s Perspective: Investigating Attachment Behaviour and the Effect of Training Method on Fear Reactions and Ease of Handling—A Pilot Study
Authors: Hartmann Elke , Rehn Therese , Christensen Janne Winther , Nielsen Per Peetz , McGreevy Paul
Published: February 2021

The study investigated equine responses to novelty and handling, aiming to reveal whether horse–human relationships reflect criteria of an attachment bond. Twelve adult Standardbreds were subjected to a fear-eliciting test (novel objects presented close to two humans) and a handling test (being led passing novel objects) to study attachment-related behaviours and ease of handling. The tests were performed both before (pre-test) and after (post-test) horses had been trained by the same female handler (10 sessions of 15 min). Horses were assigned to three groups of four, each of which underwent different operant conditioning protocols: negative reinforcement (NR; pressure, release of lead, and whip tap signals) or combined NR with either positive reinforcement using food (PRf) or wither scratching (PRs). Results showed that neither familiarity of the person nor training method had a significant impact on the horses’ behavioural responses in the post-tests. However, horses showed decreased heart rates between pre- and post-tests, which may indicate habituation, an effect of training per se, or that the presence of the familiar trainer served to calm the horses during the challenging situations. There were large individual variations among the horses’ responses and further studies are needed to increase our understanding of horse–human relationships.
A Review of Research on Dropwise Condensation Heat Transfer
Authors: Hu Xuechao , Yi Qiujie , Kong Xiangqiang , Wang Jianwei
Published: February 2021

Dropwise condensation is considered to be an effective method of enhancing heat transfer due to its high heat transfer performance. However, because the effect of dropwise condensation is affected by many complex factors, there is no systematic review summarized on the law of dropwise condensation heat transfer by scholars. In this paper, the main methods and problems of promoting dropwise condensation were reviewed based on the dropwise condensation mechanism and theoretical model. The three different hypotheses about the mechanism of dropwise condensation and the heat transfer model of dropwise condensation based on the hypothesis of nucleation sites were summarized. The methods for promoting dropwise condensation and the problems that influence dropwise condensation heat transfer are introduced in this paper. The research showed that many researchers focused on how the surface fabricated forms dropwise condensation rather than whether it enhances heat transfer. In this paper, we point out that the droplet shedding rate is the key to enhancing dropwise condensation heat transfer. Much more research on droplet formation mechanisms and theoretical models of different surfaces is supposed to be carried out in the future.
Effects of Cropland Expansion on the Regional Land Surface Radiative Energy Balance and Heat Fluxes in Northern China
Authors: Ning Jia
Published: February 2021

Land use change can impact the land surface radiation budget and energy balance by changing surface biophysical processes. Based on satellite remote sensing data and land use data from 2000 to 2015, we quantitatively estimated radiative forcing induced by cropland expansion during the early 21st century in northern China. The results showed that heat flux from the land surface to the atmosphere due to cropland expansion was quite variable in different climate zones. The heat flux increased in humid North China, whereas it decreased in arid Northwest China, semiarid Inner Mongolia, and humid Northeast China. Cropland expansion from woodland areas led to a general decline in the land surface heat flux to the atmosphere, which led to a cooling effect on the climate. The surface heat flux to the atmosphere due to cropland expansion in grassland areas displayed significant variations in different climate zones. The surface heat flux decreased only in humid Northeast China and arid Northwest China. The net surface radiation and latent heat flux both increased when grasslands were changed into cropland, but to different extents, which produced the differences in the surface heat flux to the atmosphere between different zones.
Study on the Behavior and Removal of Cadmium and Zinc Using Taraxacum officinale and Gazania under the Application of Biodegradable Chelating Agents
Authors: Kano Naoki , Hori Takumi , Zhang Haixin , Miyamoto Naoto , Anak David Eva Vanessa , Mishima Kenji
Published: February 2021

The removal of cadmium (Cd) and zinc (Zn) from soil by phytoremediation was investigated using Taraxacum officinale and Gazania. A plant environmental control system was used to cultivate the plants. The effects of different biodegradable chelating agents (i.e., EDDS, HIDS, and GLDA), relative humidity, and other competitive metals on the adsorption of Cd and Zn were also studied. In addition, the approach for metal recovery was explored by extraction of metals from plants after phytoremediation using Gazania. The concentrations of Cd and Zn were determined by inductively coupled plasma mass spectrometry (ICP-MS). In addition, one-way analysis of variance (ANOVA) tests were performed.to determine significant differences between the experimental treatments adopted in this work. Consequently, the following main conclusions were obtained: (1) In the case of Taraxacum officinale, Cd and Zn could be removed even under the presence of other heavy metals. (2) By adding a chelating agent, the amount absorbed by the shoot generally increased. (3) In the case of Gazania, the concentration of Cd was higher in root than that in shoot, whereas the concentration of Zn was higher in the shoot than that in the root. (4) Taraxacum officinale was more suitable for phytoremediation of Cd than Gazania. (5) Cd and Zn could be extracted from plants by adding a low concentration of nitric acid. (6) The one-way ANOVA tests showed no statistically significant differences among the experimental treatments.
Optimization of Ingredients for Biscuits Enriched with Rapeseed Press Cake—Changes in Their Antioxidant and Sensory Properties
Authors: Szydłowska-Czerniak Aleksandra , Poliński Szymon , Momot Monika
Published: February 2021

The optimum formulation for wheat flour (WF)-based biscuits containing the rapeseed press cake (RPC)—the primary by-product of rapeseed oil production rich in phenolic compounds and different types of fats (rapeseed oil, margarine and coconut oil)—was estimated using the central composite design (CCD) with two factors and response surface methodology (RSM). Effects of partial substitution of WF for RPC (0–40 g) in a total flour blend (100 g) and fats with various amounts of saturated fatty acids (SAFA = 2.3–24.9 g) on antioxidant capacity (AC) and sensory characteristics (color, odor, texture, flavor, overall acceptability, and purchase intent scores) of the novel biscuits were investigated. Conventional solid (liquid)–liquid extraction and ultrasound-assisted extraction (UAE) were applied to extract total antioxidants from main ingredients used for the preparation of doughs as well as the baked biscuits. The AC of biscuits and their components were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The DPPH results were the highest for the RPC flour (DPPH = 15,358–15,630 μmol Trolox (TE)/100 g) and biscuits containing rapeseed oil and 40 g of RPC flour (DPPH = 7395–10,088 μmol TE/100 g). However, these biscuits had lower sensory scores for each attribute and the lowest purchase intent scores. The quadratic response surfaces were drawn from the mathematical models in order to ensure the good quality of the proposed biscuits with RPC. The DPPH results obtained and the mean sensory scores correlate with the predicted values (R2 = 0.7751–0.9969). The addition of RPC with high antioxidant potential to biscuits and the replacement of margarine or coconut oil by rapeseed oil interfered with their acceptability.
A Method for the Design and Optimization of Nonlinear Tuned Damping Concepts to Mitigate Self-Excited Drill String Vibrations Using Multiple Scales Lindstedt-Poincaré
Authors: Kulke Vincent , Thunich Paul , Schiefer Frank , Ostermeyer Georg-Peter
Published: February 2021

In downhole drilling systems, self-excited torsional vibrations caused by the bit-rock interactions can affect the drilling process and lead to the premature failure of components. Especially self-excited oscillations of higher-order modes lead to critical dynamic loads. The slim drill string design and the naturally limited drilled borehole diameter limit the installation space, power supply and lead to numerous potentially critical self-excited torsional modes. Consequently, small and robust passive damping concepts are required. The variety of possible downhole boundary conditions and potential damper designs necessitates analytical solutions for effective damper design and optimization. In this paper, two nonlinear passive damper concepts are investigated regarding design and effectiveness to reduce self-excited high-frequency torsional oscillations in drill string dynamics. Based on a finite element model of a drill string, a suitable minimal model based on the identified critical mode is generated and solved analytically using the Multiple Scales Lindstedt-Poincaré (MSLP) method. The advantages of MSLP compared to conventional MS methods are shown for this example. On the basis of the analytical solution, parameter influences are determined, and design equations are derived. The analytical results are transferred to self-excited drill string vibrations and discussed using time domain simulations of the drill string model.
Thermal and Electrochemical Properties of Solid Polymer Electrolytes Prepared via Lithium Salt-Catalyzed Epoxide Ring Opening Polymerization
Authors: Foran Gabrielle , Verdier Nina , Lepage David , Prébé Arnaud , Aymé-Perrot David , Dollé Mickaël
Published: February 2021

Solid polymer electrolytes have been widely proposed for use in all solid-state lithium batteries. Advantages of polymer electrolytes over liquid and ceramic electrolytes include their flexibility, tunability and easy processability. An additional benefit of using some types of polymers for electrolytes is that they can be processed without the use of solvents. An example of polymers that are compatible with solvent-free processing is epoxide-containing precursors that can form films via the lithium salt-catalyzed epoxide ring opening polymerization reaction. Many polymers with epoxide functional groups are liquid under ambient conditions and can be used to directly dissolve lithium salts, allowing the reaction to be performed in a single reaction vessel under mild conditions. The existence of a variety of epoxide-containing polymers opens the possibility for significant customization of the resultant films. This review discusses several varieties of epoxide-based polymer electrolytes (polyethylene, silicone-based, amine and plasticizer-containing) and to compare them based on their thermal and electrochemical properties.
The Influence of Boiling on the Streamlined Body Drag Force and Falling Velocity
Authors: Paukštaitis Linas , Kilikevičius Sigitas , Česnavičius Ramūnas , Liutkauskienė Kristina , Ždankus Tadas
Published: February 2021

This article presents the results of numerical investigation of the influence of the streamlined body temperature on drag force and on the falling velocity in a water channel. The experimental data reflecting the cooling dynamics and body temperature influence on the falling velocity are presented as well. k − ε turbulence model and homogenous heat transfer model were chosen for the numerical 3D simulation. Drag force changes induced by the alteration of the body temperature were investigated. Velocity of the streamlined body under different temperatures of water was investigated experimentally, and the results were compared to the data obtained during the numerical simulation. The increase of the falling velocity and decrease of drag force were found to have been affected by the increase of the body temperature, which had influence on the change of the water parameters (density, phase, etc.) near the surface of the body. Simulation showed that the drag force and a velocity also depended on the water temperature. The drag force of the streamlined body decreased by 32% in comparison to the cold body for the body temperature equal to 150 °C and water temperature close to the saturation temperature (98 °C). Experimentally, it was determined that the velocity of the streamlined body covered by vapor film depended on the falling time and increased by 10–30%. Velocity difference was very small for the cold and hot bodies at the initial moment of the drop; however, it reached 20% and more after 0.3 s of the falling process.
Investigation of an Intense Dust Outbreak in the Mediterranean Using XMed-Dry Network, Multiplatform Observations, and Numerical Modeling
Authors: Rizza Umberto , Kandler Konrad , Eknayan Melanie , Passerini Giorgio , Mancinelli Enrico , Virgili Simone , Morichetti Mauro , Nolle Michael , Eleftheriadis Konstantinos , Vasilatou Vasiliki , Ielpo Pierina
Published: February 2021

The Weather Research and Forecasting (WRF) model with online coupled chemistry (WRF-Chem) is applied to study an intense Saharan dust outbreak event affecting the Italian peninsula in 15 and 16 April 2018. According to the MODIS retrievals, this intrusion was characterized by an intense aerosol optical depth (AOD) peak value in the southern Mediterranean. Measurements within the Dry Deposition Network Across the Mediterranean (XMed-Dry) are compared with the output of the WRF-Chem model. XMed-Dry samples from Lecce (Italy), Athens (Greece) and San Lawrenz/Gozo (Malta) were analysed with respect to aerosol particle size distribution, relative dust contribution, and composition. The discrepancy between the model and measured deposition indicate the need to formulate in WRF-Chem more sophisticated deposition schemes, this will need to evaluate the sensitivity of the results to the precise particle size limits chosen for the aerosol model. Moreover, satellite retrievals from MODIS sensors elaborated with the MAIAC algorithm, Aeronet stations, and measurements of PM10 at the selected sites were also considered. In a numerical domain that spans the Mediterranean and the northern Saharan desert, two different dust emission schemes, namely Gocart-AFWA and the Shao-2001, were tested and compared with multiplatform observations for simulation period covering the dust outbreak. Actual results indicate that both emission schemes would benefit from replacing the static erodibility map and soil particle distribution with remote sensed and in-situ observational data.
Detection of Density Changes in Soils with Impedance Spectroscopy
Authors: Clemens Christoph , Radschun Mario , Jobst Annette , Himmel Jörg , Kanoun Olfa
Published: February 2021

Measurement of soil parameters, such as moisture, density and density change, can provide important information for evaluating the stability of earthwork structures and for structural health monitoring. To ensure the stability of flood protection dikes, erosion at the contact zones of different soil zones must be avoided. In this work we propose the use of impedance spectroscopy to measure changes in density and volume caused by contact erosion. Erosion leads generally to a volume decrease in the contact zones between soils with different grain sizes and, consequently, to cavities in the dike structure. For this purpose, a proctor mould was developed for emulating contact erosion and the realisation of impedance measurements. Experimental investigations show a correlation between volume change of the soils in the proctor mould and impedance value. For a volume change of soil in the range of approximately 1.5% to 5.3%, an impedance change arises in the range of 17.2% to 29.8%. With several investigations we proof, that it is possible to detect material transport by impedance spectroscopy.
Listener-Position and Orientation Dependency of Auditory Perception in an Enclosed Space: Elicitation of Salient Attributes
Authors: Băcilă Bogdan Ioan , Lee Hyunkook
Published: February 2021

This paper presents a subjective study conducted on the perception of auditory attributes depending on listener position and head orientation in an enclosed space. Two elicitation experiments were carried out using the repertory grid technique—in-situ and laboratory experiments—which aimed to identify perceptual attributes among 10 different combinations of the listener’s positions and head orientations in a concert hall. It was found that, between the in-situ and laboratory experiments, the listening positions and head orientations were clustered identically. Ten salient perceptual attributes were identified from the data obtained from the laboratory experiment. Whilst these included conventional attributes such as ASW (apparent source width) and LEV (listener envelopment), new attributes such as PRL (perceived reverb loudness), ARW (apparent reverb width) and Reverb Direction were identified, and they are hypothesised to be sub-attributes of LEV (listener envelopment). Timbral characteristics such as Reverb Brightness and Echo Brightness were also identified as salient attributes, which are considered to potentially contribute to the overall perceived clarity.
Design for Additive Manufacturing: Tool Review and a Case Study
Authors: Moreno Nieto Daniel , Moreno Sánchez Daniel
Published: February 2021

This paper aims to collect in a structured manner different computer-aided engineering (CAE) tools especially developed for additive manufacturing (AM) that maximize the capabilities of this technology regarding product development. The flexibility of the AM process allows the manufacture of highly complex shapes that are not possible to produce by any other existing technology. This fact enables the use of some existing design tools like topology optimization that has already existed for decades and is used in limited cases, together with other novel developments like lattice design tools. These two technologies or design approaches demand a highly flexible manufacturing system to be applied and could not be used before, due to the conventional industrial process limitations. In this paper, these technologies will be described and combined together with other generic or specific design tools, introducing the study case of an additive manufactured mechanical design of a bicycle stem.
First Multiplatform Application for Pharmacies in Spain, Which Guides the Prescription of Probiotics According to Pathology
Authors: Alvarez-Gonzalez Sara , Rodriguez-Fernandez Jose , Porto-Pazos Ana Belen , Pazos Alejandro , Cedron Francisco
Published: February 2021

The study of the intestinal microbiota is one of the biggest challenges in the current clinical environment. In this context, probiotics have been a focus of interest to achieve the stability of the intestinal microbiota, due to probiotics’ key role in its regulation. The development of an automated system that allows practitioners to easily search for the optimal probiotic is the main objective of this study. Although it is true that there have been previous attempts of applications with this purpose, only authorized probiotics available in the countries of origin, Canada and the USA, were included. This event was a limitation when looking for those endorsed in other countries such as Spain. Thus, a system has been developed from free and multiplatform technologies that allow its use without any cost, finding, in a simple way, those probiotics that would be ideal for each pathology, either from a browser or from a cell phone.
Recognition of Handwritten Arabic and Hindi Numerals Using Convolutional Neural Networks
Authors: Alqudah Amin , Alqudah Ali Mohammad , Alquran Hiam , Al-Zoubi Hussein R. , Al-Qodah Mohammed , Al-Khassaweneh Mahmood A.
Published: February 2021

Arabic and Hindi handwritten numeral detection and classification is one of the most popular fields in the automation research. It has many applications in different fields. Automatic detection and automatic classification of handwritten numerals have persistently received attention from researchers around the world due to the robotic revolution in the past decades. Therefore, many great efforts and contributions have been made to provide highly accurate detection and classification methodologies with high performance. In this paper, we propose a two-stage methodology for the detection and classification of Arabic and Hindi handwritten numerals. The classification was based on convolutional neural networks (CNNs). The first stage of the methodology is the detection of the input numeral to be either Arabic or Hindi. The second stage is to detect the input numeral according to the language it came from. The simulation results show very high performance; the recognition rate was close to 100%.
Agricultural Drought Trends on the Iberian Peninsula: An Analysis Using Modeled and Reanalysis Soil Moisture Products
Authors: Almendra-Martín Laura , Martínez-Fernández José , González-Zamora Ángel , Benito-Verdugo Pilar , Herrero-Jiménez Carlos Miguel
Published: February 2021

Drought has a great impact on agriculture and food security, and climate change is increasing its frequency and exacerbating its intensity. Given the enormous interest in studying the recent drought evolution, in this work, agricultural drought trends over the past four decades on the Iberian Peninsula (IP) were analyzed. A trend analysis was performed with soil moisture based on the study of the evolution of anomalies and the Soil Water Deficit Index (SWDI). Two soil moisture databases (Lisflood and ERA5-Land) were used and the analysis was performed at daily and weekly temporal scales. Climate characteristics and soil properties were also considered to detect whether a trend spatial pattern could be identified. The results have shown a clear predominance of negative trends. A marked temporal pattern with negative trends was obtained over a 10-month period that coincided with the growing season of most of the crops on the IP, while a positive trend was observed over 2 months. No differences were found based on the climatic zone or soil characteristics. However, negative trends were observed to decrease as the clay content increased. These results can provide useful information for better water management and agriculture of the IP and other Mediterranean areas.
Enhanced Piezoelectric Fibered Extracellular Matrix to Promote Cardiomyocyte Maturation and Tissue Formation: A 3D Computational Model
Authors: Urdeitx Pau , Doweidar Mohamed H.
Published: February 2021

Mechanical and electrical stimuli play a key role in tissue formation, guiding cell processes such as cell migration, differentiation, maturation, and apoptosis. Monitoring and controlling these stimuli on in vitro experiments is not straightforward due to the coupling of these different stimuli. In addition, active and reciprocal cell–cell and cell–extracellular matrix interactions are essential to be considered during formation of complex tissue such as myocardial tissue. In this sense, computational models can offer new perspectives and key information on the cell microenvironment. Thus, we present a new computational 3D model, based on the Finite Element Method, where a complex extracellular matrix with piezoelectric properties interacts with cardiac muscle cells during the first steps of tissue formation. This model includes collective behavior and cell processes such as cell migration, maturation, differentiation, proliferation, and apoptosis. The model has employed to study the initial stages of in vitro cardiac aggregate formation, considering cell–cell junctions, under different extracellular matrix configurations. Three different cases have been purposed to evaluate cell behavior in fibered, mechanically stimulated fibered, and mechanically stimulated piezoelectric fibered extra-cellular matrix. In this last case, the cells are guided by the coupling of mechanical and electrical stimuli. Accordingly, the obtained results show the formation of more elongated groups and enhancement in cell proliferation.
Modeling of Old Scars: Histopathological, Biochemical and Thermal Analysis of the Scar Tissue Maturation
Authors: Fayzullin Alexey , Ignatieva Natalia , Zakharkina Olga , Tokarev Mark , Mudryak Daniil , Khristidis Yana , Balyasin Maxim , Kurkov Alexandr , Churbanov Semyon , Dyuzheva Tatyana , Timashev Peter , Guller Anna , Shekhter Anatoly
Published: February 2021

Mature hypertrophic scars (HSs) remain a challenging clinical problem, particularly due to the absence of biologically relevant experimental models as a standard rabbit ear HS model only reflects an early stage of scarring. The current study aims to adapt this animal model for simulation of mature HS by validating the time of the scar stabilization using qualitative and quantitative criteria. The full-thickness skin and perichondrium excision wounds were created on the ventral side of the rabbit ears. The tissue samples were studied on post-operation days (PODs) 30, 60, 90 and 120. The histopathological examination and morphometry were applied in parallel with biochemical analysis of protein and glycosaminoglycans (GAGs) content and amino acid composition. The supramolecular organization of collagen was explored by differential scanning calorimetry. Four stages of the rabbit ear HS maturation were delineated and attributed with the histolomorphometrical and physicochemical parameters of the tissue. The experimental scars formed in 30 days but stabilized structurally and biochemically only on POD 90–120. This evidence-based model can be used for the studies and testing of new treatments of the mature HSs.
Effect of Rubus idaeus Extracts in Murine Chondrocytes and Explants
Authors: Bourmaud Morgane , Zarka Mylene , Le Cozannet Romain , Fança-Berthon Pascale , Hay Eric , Cohen-Solal Martine
Published: February 2021

Osteoarthritis is characterized by cartilage loss resulting from the activation of chondrocytes associated with a synovial inflammation. Activated chondrocytes promote an increased secretion of matrix proteases and proinflammatory cytokines leading to cartilage breakdown. Since natural products possess anti-inflammatory properties, we investigated the direct effect of Rubus idaeus extracts (RIE) in chondrocyte metabolism and cartilage loss. The effect of RIE in chondrocyte metabolism was analyzed in murine primary chondrocytes and cartilage explants. We also assessed the contribution of RIE in an inflammation environment by culturing mice primary chondrocytes with the supernatant of Raw 264.7 macrophage-like cells primed with RIE. In primary chondrocytes, RIE diminished chondrocyte hypertrophy (Col10), while increasing the expression of catabolic genes (Mmp-3, Mmp-13) and reducing anabolic genes (Col2a1, Acan). In cartilage explants, Rubus idaeus prevented the loss of proteoglycan (14.84 ± 3.07% loss of proteoglycans with IL1 alone vs. 3.03 ± 1.86% with IL1 and 100 µg/mL of RIE), as well as the NITEGE neoepitope expression. RIE alone reduced the expression of Il1 and Il6 in macrophages, without changes in Tnf and Cox2 expression. The secretome of macrophages pre-treated with RIE and transferred to chondrocytes decreases the gene and protein expression of Mmp-3 and Cox2. In conclusion, these data suggest that RIE may protect from chondrocyte catabolism and cartilage loss in inflammatory conditions. Further evaluations are need before considering RIE as a candidate for the treatment for osteoarthritis.
Profiles of Secondary Metabolites (Phenolic Acids, Carotenoids, Anthocyanins, and Galantamine) and Primary Metabolites (Carbohydrates, Amino Acids, and Organic Acids) during Flower Development in Lycoris radiata
Authors: Park Chang Ha , Yeo Hyeon Ji , Kim Ye Jin , Nguyen Bao Van , Park Ye Eun , Sathasivam Ramaraj , Kim Jae Kwang , Park Sang Un
Published: February 2021

This study aimed to elucidate the variations in primary and secondary metabolites during Lycorisradiata flower development using high performance liquid chromatography (HPLC) and gas chromatography time-of-flight mass spectrometry (GC-TOFMS). The result showed that seven carotenoids, seven phenolic acids, three anthocyanins, and galantamine were identified in the L. radiata flowers. Most secondary metabolite levels gradually decreased according to the flower developmental stages. A total of 51 metabolites, including amines, sugars, sugar intermediates, sugar alcohols, amino acids, organic acids, phenolic acids, and tricarboxylic acid (TCA) cycle intermediates, were identified and quantified using GC-TOFMS. Among the hydrophilic compounds, most amino acids increased during flower development; in contrast, TCA cycle intermediates and sugars decreased. In particular, glutamine, asparagine, glutamic acid, and aspartic acid, which represent the main inter- and intracellular nitrogen carriers, were positively correlated with the other amino acids and were negatively correlated with the TCA cycle intermediates. Furthermore, quantitation data of the 51 hydrophilic compounds were subjected to partial least-squares discriminant analyses (PLS-DA) to assess significant differences in the metabolites of L. radiata flowers from stages 1 to 4. Therefore, this study will serve as the foundation for a biochemical approach to understand both primary and secondary metabolism in L. radiata flower development.
Development of Standardized Fetal Progenitor Cell Therapy for Cartilage Regenerative Medicine: Industrial Transposition and Preliminary Safety in Xenogeneic Transplantation
Authors: Laurent Alexis , Abdel-Sayed Philippe , Ducrot Aurélie , Hirt-Burri Nathalie , Scaletta Corinne , Jaccoud Sandra , Nuss Katja , de Buys Roessingh Anthony S. , Raffoul Wassim , Pioletti Dominique , von Rechenberg Brigitte , Applegate Lee Ann , Darwiche Salim
Published: February 2021

Diverse cell therapy approaches constitute prime developmental prospects for managing acute or degenerative cartilaginous tissue affections, synergistically complementing specific surgical solutions. Bone marrow stimulation (i.e., microfracture) remains a standard technique for cartilage repair promotion, despite incurring the adverse generation of fibrocartilagenous scar tissue, while matrix-induced autologous chondrocyte implantation (MACI) and alternative autologous cell-based approaches may partly circumvent this effect. Autologous chondrocytes remain standard cell sources, yet arrays of alternative therapeutic biologicals present great potential for regenerative medicine. Cultured human epiphyseal chondro-progenitors (hECP) were proposed as sustainable, safe, and stable candidates for chaperoning cartilage repair or regeneration. This study describes the development and industrial transposition of hECP multi-tiered cell banking following a single organ donation, as well as preliminary preclinical hECP safety. Optimized cell banking workflows were proposed, potentially generating millions of safe and sustainable therapeutic products. Furthermore, clinical hECP doses were characterized as non-toxic in a standardized chorioallantoic membrane model. Lastly, a MACI-like protocol, including hECPs, was applied in a three-month GLP pilot safety evaluation in a caprine model of full-thickness articular cartilage defect. The safety of hECP transplantation was highlighted in xenogeneic settings, along with confirmed needs for optimal cell delivery vehicles and implantation techniques favoring effective cartilage repair or regeneration.