bluuebunny/crossref_metadata_2025 huggingface dataset:
Spatial Variations in Relationships between Urbanization and Carbon Emissions in Chinese Urban Agglomerations
Authors: Li Weisong , Wu Jiahui , Yang Liyan , Chen Wanxu , Cui Xinghua , Lin Mingyu
Published: August 2024

Urban agglomerations (UAs) are the main battlefield of urbanization and the most concentrated areas of carbon emissions (CEs). Nevertheless, limited studies have examined the impact of urbanization level (UL) on CEs in UAs in China. This study aimed to identify the spatial relationship between UL and CEs in Chinese UAs and to conduct a comprehensive analysis of the differences in CEs caused by urbanization. The findings would provide scientific support for the China’s dual-carbon goals and the achievement of green and low-carbon urban development. Spatial variations in UL and CEs in 19 Chinese UAs were assessed in 2000, 2010, and 2020 using distribution dynamics and spatial regression models. The results indicated that the UL of UAs in China evidently increased over time, and UAs contributed approximately 80% of the national CEs. Significant spatial dependence was identified between urbanization factors and CEs. The regression results indicated that an increase in UL promoted the growth of CEs, and the form of the urban land had a significant and highly variable impact on CEs. Our findings provide a valuable case study for exploring relationships between UL and CEs in other UAs worldwide.
Tensile Properties of Cryorolled Cu/Al Clad Sheet with an SUS304 Interlayer after Annealing at Various Temperatures
Authors: Xuan Yanni , Li Jing , Gao Haitao , Yu Hailiang
Published: August 2024

This paper investigates the tensile properties and microstructures of Cu/Al clad sheets with an SUS304 interlayer after cryorolling and subsequent annealing and compares them with hot-rolled samples. The experimental results show that the inhibition of dynamic recovery by cryorolling enables the Cu/Al clad sheets to achieve a tensile strength of 302 MPa. After annealing, the tensile strength sharply drops to 159 MPa, while the elongation recovers to 29.0%. Compared with hot-rolled samples, the tensile strength of cryorolled samples is increased by 13.1% due to the effect of fine-grain strengthening. During the annealing process, the cryorolled samples exhibit improved elongation under a comparable strength with the hot-rolled samples, profiting from the higher degree of recrystallization and a higher proportion of annealing twins. The tensile properties of Cu/Al clad sheet with an SUS304 interlayer are strengthened by cryorolling and subsequent annealing, providing a new method for the fabrication of high-performance Cu/Al clad sheets.
Forced-Vibration Characteristics of Bowtie-Shaped Honeycomb Composite Sandwich Panel with Viscoelastic Damping Layer
Authors: Miao Siqi , Zhong Yifeng , Zhang Mingtao , Liu Rong
Published: August 2024

The incorporation of viscoelastic layers in laminates can markedly enhance the damped dynamic characteristics. This study focuses on integrating viscoelastic layers into the composite facesheet of the bowtie-shaped honeycomb core composite sandwich panel (BHC-CSP). The homogenization of the damped BHC-CSP is performed by employing the variational asymptotic method. Based on the generalized total energy equation, the energy functional of the representative unit cell of the damped BHC-CSP is asymptotically analyzed. The warping function, derived following the principle of minimum potential energy, provides a basis for obtaining the corresponding Euler–Lagrange equation to ascertain the equivalent elastic properties of the damped BHC-CSP. Utilizing the developed two-dimensional equivalent model, the free-vibration characteristics of the damped BHC-CSP are examined across diverse boundary conditions while delving into the impact of an external viscous damping layer on the natural frequency of the damped BHC-CSP. The results reveal that intensified boundary constraints effectively diminish the effective vibration region of the damped BHC-CSP, thereby enhancing its overall stability. The introduction of a PMI foam layer proves effective in adjusting the stiffness and mass distribution of the damped BHC-CSP. Resonance characteristics are explored through frequency and time-domain analyses, highlighting the pivotal roles of the excitation position and receiver point in influencing the displacement and velocity responses. Although the stiffness is improved by incorporating a PMI foam layer, its effect on the damping performance of the damped BHC-CSP is minimal when compared to the T-SW308 foam layer.
Specific Erosion Resistance Behaviour of Cold Forged and Angular Extruded Aluminium
Authors: Keran Zdenka , Jakovljević Suzana , Runje Biserka , Ciganović Igor , Ćorić Danko
Published: August 2024

Aluminium finds wide application in mechanical engineering due to its low density and corrosion resistance. In this research, aluminium was subjected to two different metal forming technologies—cold forging (upsetting) and equal channel angular pressing (ECAP)—to obtain improvement in its exploitation properties. Parallel to changing mechanical properties by using these two processes, there was a change in the microstructure of the material. The resulting microstructures were examined using an optical microscope. A different treated aluminium was subjected to erosion wear in various time intervals. Wear testing was conducted for two different impingement angles causing abrasive wear and impact wear. The erosion mechanisms were examined by scanning electron microscopy. These results showed that there is no statistically significant difference in erosion wear for different states at the same impingement angle. However, the difference is noticeable at different wear angles. The significance of the difference in wear of the samples treated with the forging and ECAP techniques was validated by statistical analysis with tests of different sensitivities. The results of the t-test showed that ECAPed samples present a statistically significant difference in the loss of mass due to variations in erosion angle during the 30, 45, and 60 min wearing. A substantial difference in the change in sample mass is also visible for the forged state worn for 60 min.
Effects of an LPSO Phase Induced by Zn Addition on the High-Temperature Properties of Mg-9Gd-2Nd-(1.5Zn)-0.5Zr Alloy
Authors: Li Ming , Yao Mengling , Liu Liangzhi , Zhang Xiaoxia , Xing Zhihui , Xia Xiangsheng , Liu Peng , Wan Yuanyuan , Chen Qiang , Wang Hongxia
Published: August 2024

In this study, we prepared Mg-9Gd-2Nd-0.5Zr, referred to as alloy I, and Mg-9Gd-2Nd-1.5Zn-0.5Zr, referred to as alloy II. The effects of a long-period stacking ordered (LPSO) phase induced by Zn addition on the high-temperature mechanical properties and fracture morphology of alloy I and alloy II at different temperatures (25 °C, 200 °C, 225 °C, and 250 °C) were studied using optical microscopy (OM), scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS), electron backscatter diffraction (EBSD), and transmission electron microscopy (TEM). The results indicate that Mg5RE at the crystal boundary of the as-cast alloy I transformed into (MgZn)3RE (as-cast alloy II) by the addition of Zn. After solid solution treatment, the secondary phase in alloy I completely disappeared, and there were still residual secondary phases in block-like and needle-like structures in alloy II, while layered LPSO phases precipitated in the matrix. During the high-temperature tensile test, the yield and tensile strength of alloy I decreased significantly with the increase in temperature, while the elongation increased. Compared to alloy I, the yield strength of alloy II with an LPSO phase showed an increasing trend at 25 °C~200 °C and then decreased when the temperature reached around 250 °C. The thermal stability was significantly enhanced, and the elongation was also higher than that of alloy I. As the temperature increased, the fracture surface of alloy I showed increased folding, bending of scratches, and crack enlargement. However, the fracture surface of alloy II remained largely unchanged, with only minor wrinkles and cracks appearing at temperatures reaching 250 °C.
The Stiffness and Damping Characteristics of a Rubber-Based SMA Composite Shock Absorber with a Hyper-Elastic SMA-Constitutive Model Considering the Loading Rate
Authors: Huang Yizhe , Zhang Huizhen , Fan Qiyuan , Huang Qibai , Shao Lefei , Zhan Xin , Wang Jun
Published: August 2024

Shock absorbers are essential in enhancing vehicle ride comfort by mitigating vibrations. However, traditional rubber shock absorbers are constrained by their fixed stiffness and damping properties, limiting their adaptability to varying loads and thus affecting the ride comfort, especially under extreme road conditions. Shape Memory Alloys (SMAs), known for their intelligent material properties, offer a unique solution by adjusting stiffness and damping in response to temperature changes or strain rates, making them ideal for advanced vibration control applications. This study builds upon the Auricchio constitutive model to propose an enhanced SMA hyper-elastic constitutive model that accounts for different loading rates. This new model elucidates the impact of loading rates on the stiffness and damping characteristics of SMAs. Additionally, we introduce an innovative circular rubber-based SMA composite vibration reduction structure. Through a parameterized model and finite element simulation, we comprehensively analyze the stiffness and damping properties of the composite damper under various loading rates and harmonic excitations. Our findings suggest a novel approach to improving the vehicle ride comfort, offering significant potential for engineering applications and practical value.
Optimized Design of Low-Carbon Mix Ratio for Non-Dominated Sorting Genetic Algorithm II Concrete Based on Genetic Algorithm-Improved Back Propagation
Authors: Zhang Fan , Wen Bo , Niu Ditao , Li Anbang , Guo Bingbing
Published: August 2024

In order to achieve low-carbon optimization in the intelligent mix ratio design of concrete materials, this work first constructs a concrete mix ratio database and performs a statistical characteristics analysis. Secondly, it employs a standard back propagation (BP) and a genetic algorithm-improved BP (GA-BP) to predict the concrete mix ratio. The NSGA-II algorithm is then used to optimize the mix ratio. Finally, the method’s accuracy is validated through experiments. The study’s results indicate that the statistical characteristics of the concrete mix ratio data show a wide distribution range and good representativeness. Compared to the standard BP, the fitting accuracies of each GA-BP set are improved by 4.9%, 0.3%, 16.7%, and 4.6%, respectively. According to the Fast Non-Dominated Sorting Genetic Algorithm II (NSGA-II) optimization for meeting C50 concrete strength requirements, the optimal concrete mix ratio is as follows: cement 331.3 kg/m3, sand 639.4 kg/m3, stone 1039 kg/m3, fly ash 56 kg/m3, water 153 kg/m3, and water-reducing agent 0.632 kg/m3. The 28-day compressive strength, material cost, and carbon emissions show relative errors of 2.1%, 0.6%, and 2.9%, respectively. Compared with commercial concrete of the same strength grade, costs and carbon emissions are reduced by 7.2% and 15.9%, respectively. The methodology used in this study not only significantly improves the accuracy of concrete design but also considers the carbon emissions involved in the concrete preparation process, reflecting the strength, economic, and environmental impacts of material design. Practitioners are encouraged to explore integrated low-carbon research that spans from material selection to structural optimization.
Biolaminates as an Example of Upcycling Product with Keratin Flour—Research and Thermal Properties Modeling
Authors: Frydrysiak Michał
Published: August 2024

Keratin waste, including keratin powder, is a significant byproduct of the poultry processing and meat industries. It is a major contributor to waste management problems due to its volume and the environmental pollutants that it can produce. The disposal of keratin waste is challenging due to the potential for odors and pathogens to enter the soil and water. The aim of this work is to present the possibility of using waste materials in accordance with the principles of upcycling and producing fully valuable products. In this research, the author focuses on the production and research of textile multilayer laminates using keratin flour that had been previously considered waste material. New textile composites should be characterized by increased thermal insulation properties with constant comfort in use. This research determines the physiological comfort interpreted as the state of the human–laminate system, which maintains the conditions of comfort in human perception, i.e., constant temperature and humidity of the body under changing conditions of a relative humidity environment.
Continuum–Discontinuum Bonded-Block Model for Simulating Mixed-Mode Fractures
Authors: Sun Yue , Chen Tao , Yong Longquan , Chen Qian
Published: August 2024

In this study, an improved discontinuous deformation analysis method with sub-block strategy is introduced to numerically simulate mixed-mode fractures. This approach partitions the material domain into continuum and potential discontinuum regions, applying specialized modeling techniques to each. In the continuum region, penalty-like bonding springs are employed to glue the sub-blocks together to capture the elastic behavior of the material. In the potential discontinuum region, the cohesive springs with the stiffness based on the cohesive zone model are implemented between sub-blocks to reproduce the process of crack nucleation and propagation. The primary advantage of this method is its capability to effectively model the transition of quasi-brittle solids from a continuous to a discontinuous stage through the degradation of cohesive springs. This accurately represents material failure while maintaining stability and consistency along uncracked interfaces. Another significant benefit is the method’s efficiency, as it avoids complex contact operations along sub-block interfaces before the cohesive spring between them fails. Validation through various benchmark numerical examples, such as cantilever beam-bending and diverse fracture simulations, demonstrates the method’s accuracy and robustness by comparing the results with analytical solutions. These comparisons show that the proposed method effectively captures the interplay between tensile and shear traction components in the mixed-mode crack propagation process.
Progressive Archive in Adaptive jSO Algorithm
Authors: Bujok Petr
Published: August 2024

The problem of optimisation methods is the stagnation of population P, which results in a local solution for the task. This problem can be solved by employing an archive for good historical solutions outperformed by the new better offspring. The archive A was introduced with the variant of adaptive differential evolution (DE), and it was successfully applied in many adaptive DE variants including the efficient jSO algorithm. In the original jSO, the historical good individuals replace the random existing positions in A. It causes that outperformed historical solution from P with lower quality to replace the stored solution in A with better quality. In this paper, a new approach to replace individuals in archive A more progressively is proposed. Outperformed individuals from P replace solutions in the worse part of A based on the function value. The portion of A selected for replacement is controlled by the input parameter, and its setting is studied in this experiment. The proposed progressive archive is employed in the original jSO. Moreover, the Eigenvector transformation of the individuals for crossover is applied to increase the efficiency for the rotated optimisation problems. The efficiency of the proposed progressive archive and the Eigen crossover are evaluated using the set of 29 optimisation problems for CEC 2024 and various dimensionality. All the experiments were performed on a standard PC, and the results were compared using the standard statistical methods. The newly proposed algorithm with the progressive archive approach performs substantially better than the original jSO, especially when 20 or 40% of the worse individuals of A are set for replacement. The Eigen crossover increases the performance of the proposed jSO algorithm with the progressive archive approach. The estimated time complexity illustrates the low computational demands of the proposed archive approach.
Comparative Transcriptomic Analysis on the Effect of Sesamol on the Two-Stages Fermentation of Aurantiochytrium sp. for Enhancing DHA Accumulation
Authors: Yang Xuewei , Wei Liyang , Liang Shitong , Wang Zongkang , Li Shuangfei
Published: August 2024

Aurantiochytrium is a well-known long-chain polyunsaturated fatty acids (PUFAs) producer, especially docosahexaenoic acid (DHA). In order to reduce the cost or improve the productivity of DHA, many researchers are focusing on exploring the high-yield strain, reducing production costs, changing culture conditions, and other measures. In this study, DHA production was improved by a two-stage fermentation. In the first stage, efficient and cheap soybean powder was used instead of conventional peptone, and the optimization of fermentation conditions (optimal fermentation conditions: temperature 28.7 °C, salinity 10.7‰, nitrogen source concentration 1.01 g/L, and two-nitrogen ratio of yeast extract to soybean powder 2:1) based on response surface methodology resulted in a 1.68-fold increase in biomass concentration. In the second stage, the addition of 2.5 mM sesamol increased the production of fatty acid and DHA by 93.49% and 98.22%, respectively, as compared to the optimal culture condition with unadded sesamol. Transcriptome analyses revealed that the addition of sesamol resulted in the upregulation of some genes related to fatty acid synthesis and antioxidant enzymes in Aurantiochytrium. This research provides a low-cost and effective culture method for the commercial production of DHA by Aurantiochytrium sp.
Long-Term Nitrogen Dioxide Exposure as a Possible 5-Year Mortality Risk Factor in Diabetic Patients Treated Using Off-Pump Surgical Revascularization—A Retrospective Analysis
Authors: Urbanowicz Tomasz , Skotak Krzysztof , Krasińska-Płachta Aleksandra , Kowalewski Mariusz , Olasińska-Wiśniewska Anna , Szczepański Krystian , Tykarski Andrzej , Krasińska Beata , Krasiński Zbigniew , Jemielity Marek
Published: August 2024

Background: There is mounting evidence that diabetic-related cardiac metabolism abnormalities with oxidative stress and inflammatory mechanism activation align with the functional impairments that result in atherosclerotic lesion formation. Among the possible non-traditional coronary lesion risk factors, environmental exposure may be significant, especially in diabetic patients. Methods: A total of 140 diabetic patients (115 (82%) males and 25 (18%) females) with a mean age of 65 (60–71) underwent surgical revascularization due to multivessel coronary disease. The possible all-cause mortality risk factors, including demographical and clinical factors followed by chronic air pollution exposure, were identified. Results: All patients were operated on using the off-pump technique and followed for 5.6 (5–6.1) years. The multivariable model for 5-year mortality prediction presented the nitrogen dioxide chronic exposure (HR: 3.99, 95% CI: 1.16–13.71, p = 0.028) and completeness of revascularization (HR: 0.19, 95% CI: 0.04–0.86, p = 0.031) as significant all-cause mortality risk factors. Conclusions: Ambient air pollutants such as an excessive chronic nitrogen dioxide concentration (>15 µg/m3) may increase 5-year all-cause mortality in diabetic patients following surgical revascularization.
Beneficial Bacteria in the Gut Microbiota May Lead to Improved Metabolic and Immunological Status in Chronic Obstructive Pulmonary Disease
Authors: Passos Fabine Correia , Oliveira Lucas Matheus Gonçalves de , Jesus Fabíola Ramos , Zanette Dalila Lucíola , Neto Odilon Lobão Leal , Neves Margarida Célia Lima Costa , Lemos Antônio Carlos Moreira , Baccan Gyselle Chrystina
Published: August 2024

The progression of chronic obstructive pulmonary disease (COPD) is characterized by functional changes in the airways. The lung–gut axis and gut microbiota (GM) have been linked to the pathophysiology of airway diseases. Regarding COPD, studies have shown that GM alterations could be related the stages of this disease. However, the relationship between GM and clinical, biochemical and immunological parameters in patients with COPD are not well understood. The aim of this study was to compare the relative abundance of specific groups of beneficial gut bacteria between COPD patients and healthy controls (CTLs) in order to evaluate relationships with metabolic and inflammatory markers in COPD. Methods: We included 16 stable COPD patients and 16 healthy volunteer CTLs. The relative abundances of Bifidobacterium spp. (Bf) and Akkermansia muciniphila (Akk) bacteria and the Bacteroidetes and Firmicutes phyla were assessed by qPCR. Pulmonary function was evaluated by spirometry, biochemical parameters by colorimetric methods and plasma cytokine levels by cytometric bead array analysis. Results: The Firmicutes/Bacteroides ratio was related to emergency hospital visits and six-minute walk test (6MWT) results. Furthermore, the relative abundance of Bf was associated with plasma concentrations of glucose, triglycerides, HDL-C and IL-10. In addition, Firmicutes levels and the Firmicutes/Bacteroidetes ratio were associated with the IL-12/IL-10 ratio, while Akk abundance was linked to IL-12 levels. Conclusions: The present findings suggest that the abundance of beneficial bacteria in the GM could influence clinical presentation and immunoregulation in COPD.
Bacillus spp. Isolated from Miang as Potential Probiotics in Nile Tilapia Culture—In Vitro Research
Authors: Anyairo Chioma Stella , Unban Kridsada , Wongputtisin Pairote , Rojtinnakorn Jiraporn , Shetty Kalidas , Khanongnuch Chartchai
Published: August 2024

Among 79 Bacillus spp. isolated from Miang, a fermented tea in north Thailand, 17 Bacillus strains were selected with probiotic potential in Nile tilapia culture based on the capabilities of bacteriocin production and associated antimicrobial activities against fish pathogens, Aeromonas hydrophila and Streptococcus agalactiae. However, only six isolates were selected for further extensive studies based on the strength of their antimicrobial activities and their tolerance against simulated gastrointestinal conditions. The molecular identification by 16S rRNA gene sequence analysis revealed that five isolates, K2.1, K6.1, K7.1, K15.4, and K22.6, were Bacillus tequilensis, and the isolate K29.2 was Bacillus siamensis. B. siamensis K29.2 showed complete susceptibility to antibiotics tested in this study, while B. tequilensis K 15.4 showed moderate resistance to some antibiotics; therefore, both strains were selected as potential probiotic bacteria. B. tequilensis K15.4 and B. siamensis K29.2 were capable of the production and secretion of extracellular protease and polysaccharide degrading enzymes, including cellulase, xylanase, and β-mannanase. The tannin tolerant test also demonstrated their ability to grow on selective agar plates and secrete cellulase and β-mannanase in the presence of hydrolyzable tannin. In addition, in vitro digestion of commercial fish substrate revealed that the extracellular enzymes produced by both strains efficiently reacted with feed protein and polysaccharides. Based on the results from this study, B. siamensis K29.2 was deemed to have the highest potential multifunctional probiotic qualities for application in Nile tilapia culture, while the antibiotic-resistant gene in B. tequilensis K15.4 must be clarified before field application.
Host Gut-Derived Probiotic, Exiguobacterium acetylicum G1-33, Improves Growth, Immunity, and Resistance to Vibrio harveyi in Hybrid Grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂)
Authors: Zhang Mingqing , Feng Yuwei , Zhong Zhongxuan , Du Qianping , Yu Wei , Wu Jinhui , Huang Xiaolin , Huang Zhong , Xie Guangting , Shu Hu
Published: August 2024

Several exogenous probiotics are applicable in fish culture; however, challenges in isolation and verification have hindered the full utilization of numerous host probiotics. Therefore, this study aimed to apply the host probiotic Exiguobacterium acetylicum G1-33 to hybrid grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂) cultures and explore its mechanism of action. In total, 360 hybrid grouper were divided into four groups, which were fed the following for 60 days: three received commercial feed with varying concentrations of E. acetylicum G1-33 (106, 108, and 1010 CFU/g), while a control group received commercial feed. The results showed that supplementation with 106 and 108 CFU/g of E. acetylicum G1-33 enhanced gut morphology, upregulated growth-related genes (ghr1, igf-2, s6k1, tor), and promoted growth, with supplementation with 108 CFU/g resulting in the most notable enhancement. However, supplementation with 1010 CFU/g inhibited growth, possibly because of changes in intestinal morphology. Additionally, supplementation with E. acetylicum G1-33 upregulated the expression of immune-related genes (c3, myd88, Cu/Zn-sod, tlr3, and tnf2) in the liver and head kidney but led to an increase in malondialdehyde content, as well as a decrease in alkaline phosphatase and acid phosphatase activities, in the liver and serum, indicating increased oxidative stress. Moreover, supplementation with 106 and 108 CFU/g E. acetylicum G1-33 enhanced the widespread expression of immune-related genes in the head kidney and liver, respectively, and improved resistance to Vibrio harveyi, whereas supplementation with 1010 CFU/g weakened this resistance. In conclusion, E. acetylicum G1-33, particularly at 108 CFU/g, emerged as an effective probiotic, optimizing growth performance and immunity in hybrid grouper. This research is pioneering in its application of E. acetylicum in mariculture, potentially broadening the range of probiotic strategies in aquaculture.
Antarctic Soils Select Copiotroph-Dominated Bacteria
Authors: Zhang Lujie , Zhao Xue , Wang Jieying , He Liyuan , Ren Chengjie , Wang Jun , Guo Yaoxin , Wang Ninglian , Zhao Fazhu
Published: August 2024

The life strategies of bacterial communities determine their structure and function and are an important driver of biogeochemical cycling. However, the variations in these strategies under different soil resource conditions remain largely unknown. We explored the bacterial life strategies and changes in structure and functions between Antarctic soils and forest (temperate, subtropical, and tropical) soils. The results showed that the weighted mean rRNA operon copy number in temperate soils was 19.5% lower than that in Antarctic soils, whereas no significant differences were observed among Antarctic, subtropical, and tropical soils. An unexpected result was that bacterial communities in Antarctic soils tended to be copiotrophs, such as Actinobacteriota and Bacteroidota, whereas those in temperate soils tended to be oligotrophs, such as Acidobacteriota and Chloroflexi. Functional predictions showed that in comparison to copiotrophs in Antarctic soils, temperate-inhabiting oligotrophic bacteria exhibited an 84.2–91.1% lower abundance of labile C decomposition genes (hemicellulose, cellulose, monosaccharides, and disaccharides), whereas a 74.4% higher abundance of stable C decomposition (lignin). Genes involved in N cycling (nitrogen fixation, assimilatory nitrate reduction, and denitrification) were 24.3–64.4% lower in temperate soils than in Antarctic soils. Collectively, our study provides a framework for describing the life strategies of soil bacteria, which are crucial to global biogeochemical cycles.
Advancing Bacillus licheniformis as a Superior Expression Platform through Promoter Engineering
Authors: Xiao Fengxu , Zhang Yupeng , Zhang Lihuan , Li Siyu , Chen Wei , Shi Guiyang , Li Youran
Published: August 2024

Bacillus licheniformis is recognised as an exceptional expression platform in biomanufacturing due to its ability to produce high-value products. Consequently, metabolic engineering of B. licheniformis is increasingly pursued to enhance its utility as a biomanufacturing vehicle. Effective B. licheniformis cell factories require promoters that enable regulated expression of target genes. This review discusses recent advancements in the characterisation, synthesis, and engineering of B. licheniformis promoters. We highlight the application of constitutive promoters, quorum sensing promoters, and inducible promoters in protein and chemical synthesis. Additionally, we summarise efforts to expand the promoter toolbox through hybrid promoter engineering, transcription factor-based inducible promoter engineering, and ribosome binding site (RBS) engineering.
Effect of Solid Ratio and Particle Size on Dissolution of Heat-Activated Lizardite at Elevated Pressures and Moderate Temperatures
Authors: Abu Fara Ammar , Rayson Mark R. , Brent Geoff F. , Oliver Timothy K. , Stockenhuber Michael , Kennedy Eric M.
Published: August 2024

This study investigates the effect of the particle size and solid-to-liquid ratio on the dissolution rate of magnesium (Mg) and silicon (Si) in heat-activated lizardite. The investigation was conducted under specific conditions: without the presence of sodium bicarbonate (NaHCO3), at a moderate temperature (40 °C), and under elevated CO2 pressure (100 bar). The aim was to isolate the dissolution reactions and enhance comprehension of the factors constraining the overall yields in the Albany Research Center (ARC) mineral carbonation process. Our study disclosed two distinct dissolution regimes: an initial stage with a rapid initial rate of Mg extraction, resulting in the fraction of Mg extracted ranging from 30 to 65% during the first 20 min of the experiment, following which the dissolution rate decreases dramatically. The initial rapid dissolution stage is primarily driven by the low pH of the supernatant solution, resulting from CO2 dissolution, leading to a higher concentration of protons that extract Mg2+ cations. However, as the heat-activated lizardite dissolution progresses, the pH increases due to the high level of leached Mg2+, and a diffusion barrier forms due to the precipitation of amorphous silica. This phenomenon ultimately slows down the mineral’s dissolution rate during the latter stages of particle dissolution.
An Overview of Various Applications of Cadmium Carboxylate Coordination Polymers
Authors: Vasile Scaeteanu Gina , Maxim Catalin , Badea Mihaela , Olar Rodica
Published: August 2024

This review highlights the most recent applications of Cd(II)-carboxylate-based coordination polymers (Cd(II)-CBCPs), such as sensors, catalysts, and storage materials, in comparison with those of Zn(II) counterparts. A wide range of species with luminescence properties were designed by using proper organic fluorophores, especially a carboxylate bridging ligand combined with an ancillary N-donor species, both with a rigid structure. These characteristics, combined with the arrangement in Cd(II)-CBCPs’ structure and the intermolecular interaction, enable the sensing behavior of a plethora of various inorganic and organic pollutants. In addition, the Lewis acid behavior of Cd(II) was investigated either in developing valuable heterogeneous catalysts in acetalization, cyanosilylation, Henry or Strecker reactions, Knoevenagel condensation, or dyes or drug elimination from wastewater through photocatalysis. Furthermore, the pores structure of such derivatives induced the ability of some species to store gases or toxic dyes. Applications such as in herbicides, antibacterials, and electronic devices are also described together with their ability to generate nano-CdO species.
Degradation Efficiency and Mechanism of Tetracycline in Water by Activated Persulfate Using Biochar-Loaded Nano Zero-Valent Iron
Authors: Yan Bojiao , Li Xueqi , Wang Xiaoyan , Yang Ping , Lu Hai , Zhang Xiaoyu
Published: August 2024

Tetracycline (TC) contamination in water is one of the key issues in global environmental protection, and traditional water treatment methods are difficult to remove antibiotic pollutants.Therefore, efficient and environmentally friendly treatment technologies are urgently needed. In this study, activated persulfate (PS) using a biochar-loaded nano zero-valent iron (BC-nZVI) advanced oxidation system was used to investigate the degradation effect, influencing factors, and mechanism of TC. BC-nZVI was prepared using the liquid-phase reduction method, and its structure and properties were analyzed by various characterization means. The results showed that nZVI was uniformly distributed on the surface or in the pores of BC, forming a stable complex. Degradation experiments showed that the BC-nZVI/PS system could degrade TC up to 99.57% under optimal conditions. The experiments under different conditions revealed that the iron-carbon ratio, dosing amount, PS concentration, and pH value all affected the degradation efficiency. Free radical burst and electron paramagnetic resonance (EPR) experiments confirmed the dominant roles of hydroxyl and sulfate radicals in the degradation process, and LC–MS experiments revealed the multi-step reaction process of TC degradation. This study provides a scientific basis for the efficient treatment of TC pollution in water.
Extraction, Characterization, and Antioxidant Activity of Pectin from Lemon Peels
Authors: Dambuza Anathi , Rungqu Pamela , Oyedeji Adebola Omowunmi , Miya Gugulethu M. , Kuria Simon K. , Hosu Sunday Yiseyon , Oyedeji Opeoluwa Oyehan
Published: August 2024

Pectin is a natural polymer that is found in the cell walls of higher plants. This study presents a comprehensive analysis of pectin extracted from lemon in two different geographic regions (Peddie and Fort Beaufort) in two consecutive years (2023 and 2024) named PP 2023, PP 2024, FBP 2023, and FBP 2024. The dried lemon peels were ground into a powder, sifted to obtain particles of 500 μm, and then subjected to pectin extraction using a conventional method involving mixing lemon peel powder with distilled water, adjusting the pH level to 2.0 with HCl, heating the mixture at 70 °C for 45 min, filtering the acidic extract, and precipitating pectin with ethanol. The yield of these pectin samples was statistically significant, as FBP 2024 had a maximum yield of 12.2 ± 0.02%, PP 2024 had a maximum yield of 13.0 ± 0.02%, FBP 2023 had a maximum yield of 12.2 ± 0.03%, and PP 2023 had a maximum yield of 13.1 ± 0.03%, The variation in yield could be due to the differences in the growing conditions, such as the climate and soil, which could have affected the pectin content in the lemons. The physicochemical characterization of all samples proved that our pectin samples could be used in the pharmaceutical and food industries, with anhydrouronic acid content which was greater than 65%, as suggested by the FAO. The scanning electron microscope analysis of all extracted pectin was rough and jagged, while the commercial pectin displayed a smooth surface morphology with a consistent size. FTIR confirmed the functional groups which were present in our samples. Thermogravimetric analysis was employed to investigate the thermal behavior of the extracted pectin in comparison with commercial pectin. It was found that the extracted pectin had three-step degradation while the commercial pectin had four-step degradation. Additionally, pectin samples have been shown to have antioxidants, as the IC50 of PP 2024, PP 2023, FBP 2023, FBP 2024, and Commercial P was 1062.5 ± 20.0, 1201.3 ± 22.0, 1304.6 ± 19.0, 1382.6 ± 29.9, and 1019.4 ± 17.1 mg/L, respectively. These findings indicate that lemon pectin has promising characteristics as a biopolymer for use in biomedical applications.
The Role of Lignin Molecular Weight on Activated Carbon Pore Structure
Authors: Wu Chengjun , Ding Junhuan , Tindall Graham W. , Pittman Zachariah A. , Thies Mark C. , Roberts Mark E.
Published: August 2024

Over the past decade, the production of biofuels from lignocellulosic biomass has steadily increased to offset the use of fuels from petroleum. To make biofuels cost-competitive, however, it is necessary to add value to the “ligno-” components (up to 30% by mass) of the biomass. The properties of lignin, in terms of molecular weight (MW), chemical functionality, and mineral impurities often vary from biomass source and biorefinery process, resulting in a challenging precursor for product development. Activated carbon (AC) is a feasible target for the lignin-rich byproduct streams because it can be made from nearly any biomass, and it has a market capacity large enough to use much of the lignin generated from the biorefineries. However, it is not known how the variability in the lignin affects the key properties of AC, because, until now, they could not be well controlled. In this work, various fractions of ultraclean (<0.6% ash) lignin are created with refined MW distributions using Aqueous Lignin Purification using Hot Agents (ALPHA) and used as precursors for AC. AC is synthesized via zinc chloride activation and characterized for pore structure and adsorption capacity. We show that AC surface area and the adsorption capacity increase when using lignin with increasing MW, and, furthermore, that reducing the mineral content of lignin can significantly enhance the AC properties. The surface area of the AC from the highest MW lignin can reach ~1830 m2/g (absorption capacity). Furthermore, single step activation carbonization using zinc chloride allows for minimal carbon burn off (<30%), capturing most of the lignin carbon compared to traditional burn off methods in biorefineries for heat generation.
Recent Progress in Thermoplastic Polyurethane/MXene Nanocomposites: Preparation, Flame-Retardant Properties and Applications
Authors: Yuan Yao , Lin Weiliang , Xu Lulu , Wang Wei
Published: August 2024

MXene, a promising two-dimensional nanomaterial, exhibits significant potential across various applications due to its multilayered structure, metal-like conductivity, solution processability, and surface functionalization capabilities. These remarkable properties facilitate the integration of MXenes and MXene-based materials into high-performance polymer composites. Regarding this, a comprehensive and well-structured up-to-date review is essential to provide an in-depth understanding of MXene/thermoplastic polyurethane nanocomposites. This review discusses various synthetic and modification methods of MXenes, current research progress and future potential on MXene/thermoplastic polyurethane nanocomposites, existing knowledge gaps, and further development. The main focus is on discussing strategies for modifying MXene-based compounds and their flame-retardant efficiency, with particular emphasis on understanding their mechanisms within the TPU matrix. Ultimately, this review addresses current challenges and suggests future directions for the practical utilization of these materials.
Hydrogels and Aerogels for Versatile Photo-/Electro-Chemical and Energy-Related Applications
Authors: Sun Jiana , Luo Taigang , Zhao Mengmeng , Zhang Lin , Zhao Zhengping , Yu Tao , Yan Yibo
Published: August 2024

The development of photo-/electro-chemical and flexible electronics has stimulated research in catalysis, informatics, biomedicine, energy conversion, and storage applications. Gels (e.g., aerogel, hydrogel) comprise a range of polymers with three-dimensional (3D) network structures, where hydrophilic polyacrylamide, polyvinyl alcohol, copolymers, and hydroxides are the most widely studied for hydrogels, whereas 3D graphene, carbon, organic, and inorganic networks are widely studied for aerogels. Encapsulation of functional species with hydrogel building blocks can modify the optoelectronic, physicochemical, and mechanical properties. In addition, aerogels are a set of nanoporous or microporous 3D networks that bridge the macro- and nano-world. Different architectures modulate properties and have been adopted as a backbone substrate, enriching active sites and surface areas for photo-/electro-chemical energy conversion and storage applications. Fabrication via sol–gel processes, module assembly, and template routes have responded to professionalized features and enhanced performance. This review presents the most studied hydrogel materials, the classification of aerogel materials, and their applications in flexible sensors, batteries, supercapacitors, catalysis, biomedical, thermal insulation, etc.
Potential Pharmacological Properties of Triterpene Derivatives of Ursolic Acid
Authors: Khwaza Vuyolwethu , Aderibigbe Blessing A.
Published: August 2024

Ursolic acid (UA) and its derivatives have garnered significant attention due to their extensive pharmacological activity. UA is a pentacyclic triterpenoid found in a variety of plants, such as apples, rosemary, thyme, etc., and it possesses a range of pharmacological properties. Researchers have synthesized various derivatives of UA through structural modifications to enhance its potential pharmacological properties. Various in vitro and in vivo studies have indicated that UA and its derivatives possess diverse biological activities, such as anticancer, antifungal, antidiabetic, antioxidant, antibacterial, anti-inflammatory and antiviral properties. This review article provides a review of the biological activities of UA and its derivatives to show their valuable therapeutic properties useful in the treatment of different diseases, mainly focusing on the relevant structure-activity relationships (SARs), the underlying molecular targets/pathways, and modes of action.
Fabrication Methods of Continuous Pure Metal–Organic Framework Membranes and Films: A Review
Authors: Xing Qinglei , Xu Xiangyou , Li Haoqian , Cui Zheng , Chu Binrui , Xie Nihao , Wang Ziying , Bai Peng , Guo Xianghai , Lyu Jiafei
Published: August 2024

Metal–organic frameworks (MOFs) have drawn intensive attention as a class of highly porous, crystalline materials with significant potential in various applications due to their tunable porosity, large internal surface areas, and high crystallinity. This paper comprehensively reviews the fabrication methods of pure MOF membranes and films, including in situ solvothermal synthesis, secondary growth, electrochemical deposition, counter diffusion growth, liquid phase epitaxy and solvent-free synthesis in the category of different MOF families with specific metal species, including Zn-based, Cu-based, Zr-based, Al-based, Ni-based, and Ti-based MOFs.
Chemical Characterization of Pruning Wood Extracts from Six Japanese Plum (Prunus salicina Lindl.) Cultivars and Their Antitumor Activity
Authors: Ortega-Vidal Juan , Mut-Salud Nuria , de la Torre José M. , Altarejos Joaquín , Salido Sofía
Published: August 2024

The Japanese plum tree (Prunus salicina Lindl.) is mainly cultivated in temperate areas of China and some European countries. Certain amounts of wood (from pruning works) are generated every year from this crop of worldwide commercial significance. The main objective of this work was to value this agricultural woody residue, for which the chemical composition of pruning wood extracts from six Japanese plum cultivars was investigated, and the antiproliferative activity of extracts and pure phenolics present in those extracts was measured. For the chemical characterization, total phenolic content and DPPH radical-scavenging assays and HPLC‒DAD/ESI‒MS analyses were performed, with the procyanidin (−)-ent-epicatechin-(2α→O→7,4α→8)-epicatechin (5) and the propelargonidin (+)-epiafzelechin-(2β→O→7,4β→8)-epicatechin (7) being the major components of the wood extracts. Some quantitative differences were found among plum cultivars, and the content of proanthocyanidins ranged from 1.50 (cv. ‘Fortune’) to 4.44 (cv. ‘Showtime’) mg/g of dry wood. Regarding the antitumoral activity, eight wood extracts and four phenolic compounds were evaluated in MCF-7 cells after 48 h of induction, showing the wood extract from cv. ‘Songold’ and (‒)-annphenone (3), the best antiproliferative activity (IC50: 424 μg/mL and 405 μg/mL, respectively).
Location, Location, Location: Establishing Design Principles for New Antibacterials from Ferric Siderophore Transport Systems
Authors: Luo Vivien Canran , Peczuh Mark W.
Published: August 2024

This review strives to assemble a set of molecular design principles that enables the delivery of antibiotic warheads to Gram-negative bacterial targets (ESKAPE pathogens) using iron-chelating siderophores, known as the Trojan Horse strategy for antibiotic development. Principles are derived along two main lines. First, archetypical siderophores and their conjugates are used as case studies for native iron transport. They enable the consideration of the correspondence of iron transport and antibacterial target location. The second line of study charts the rationale behind the clinical antibiotic cefiderocol. It illustrates the potential versatility for the design of new Trojan Horse-based antibiotics. Themes such as matching the warhead to a location where the siderophore delivers its cargo (i.e., periplasm vs. cytoplasm), whether or not a cleavable linker is required, and the relevance of cheaters to the effectiveness and selectivity of new conjugates will be explored. The effort to articulate rules has identified gaps in the current understanding of iron transport pathways and suggests directions for new investigations.
Long Neurocognitive and Neuropsychiatric Sequelae in Participants with Post-COVID-19 Infection: A Longitudinal Study
Authors: Almeria Marta , Cejudo Juan Carlos , Deus Joan , Krupinski Jerzy
Published: August 2024

Objective: To evaluate and characterize the cognitive changes in COVID-19 participants at 6-month follow-up, and to explore a possible association with clinical symptoms, emotional disturbance and disease severity. Methods: This single-center longitudinal cohort study included participants aged 20 and 60 years old to exclude cognitive impairment age-associated with confirmed COVID-19 infection. The initial evaluation occurred 10 to 30 days after hospital or ambulatory discharge, with a subsequent follow-up at 6 months. Patients who had a history of cognitive impairment, neurological conditions, or serious psychiatric disorders were not included. Information on demographics and laboratory results was gathered from medical records. Cognitive outcomes were assessed with a neuropsychological battery including attention, verbal and visual memory, language and executive function tests. Results: A total of 200 participants were included in the study, and 108 completed the follow-up visit. At the 6-month follow-up, comparing the means from baseline with those of the follow-up evaluation, significant overall improvement was observed in verbal and visual memory subtests (p = 0.001), processing speed (p = 0.001), executive function (p = 0.028; p = 0.016) and naming (p = 0.001), independently of disease severity and cognitive complaints. Anxiety and depression were significantly higher in groups with Subjective Cognitive Complaints (SCC) compared to those without (p < 0.01 for both). Conclusions: Persistent symptoms are common regardless of disease severity and are often linked to cognitive complaints. Six months after COVID-19, the most frequently reported symptoms included headache, dyspnea, fatigue, cognitive complaints, anxiety, and depression. No cognitive impairment was found to be associated with the severity of COVID-19. Overall, neuropsychological and psychopathological improvement was observed at 6 months regardless of disease severity and cognitive complaints.