bluuebunny/crossref_metadata_2025 huggingface dataset:
Extracellular Vesicles and their miRNA Cargo: A Means of Communication between Soma and Germline in the Mammalian Reproductive System
Authors: Maciel Emmanuelle , Mansuy Isabelle M.
Published: May 2019

MicroRNAs (miRNAs) are small non-coding RNAs able to silence gene expression by RNA interference. They are present in cells but many are contained in extracellular vesicles (EVs) that can be released by cells in the circulation. Circulating EVs can encounter other cells in the body
and deliver their miRNA cargo. This process enables long-range communication between different cells and has been proposed to play important physiological roles. One of these roles that remains less well studied is in the reproductive system. In ovaries and testes, constant communication between
somatic cells and developing germ cells is necessary for their maturation and EVs have been proposed to contribute to this communication. EVs might also enable external factors derived from environmental exposure to reach gametes and keep a trace of exposure for the offspring.
Dynamics of Membrane-Embedded Lipid-Linked Oligosaccharides for the Three Domains of Life
Authors: Arantes Pablo Ricardo , Pedebos Conrado , Pol-Fachin Laércio , D. Poleto Marcelo , Verli Hugo
Published: May 2019

Lipid-linked oligosaccharides (LLOs) are the substrates of oligosaccharyltransferases (OSTs), enzymes that catalyze the en bloc transfer of a glycan chain during the process of N-glycosylation. LLOs are composed by an isoprenoid chain moiety and an oligosaccharide, linked by one or more pyrophosphate groups (PP). The lipid component on LLO is a dolichol in eukarya and archaea, and an undecaprenol in prokarya, whereas the number of isoprene units may change between species. Given the potential relevance of LLOs and their metabolizing enzymes to diverse biotechnological applications, LLOs’ models from different domains of life in their native conditions could support further studies of their complexation and processing by OSTs, as well as protein engineering on such systems. Accordingly, the GROMOS53A6 force field was employed, added by GROMOS53a6GLYC parameters for the saccharidic moiety. The torsional parameters for the isoprenoid portion were derived from a fit to the proper quantum mechanical potential energy profiles at the HF 6-31G* and validated against experimental condensed phase properties. Molecular dynamics simulations employed GROMACS package to access the orientation, structure, and dynamics of eukaryotic (Glc3-Man9-GlcNAc2-PP-Dolichol), bacterial (Glc1-GalNAc5-Bac1-PP-Undecaprenol) and archaeal (Glc1-Man1-Gal1-Man1-Glc1-Gal1-Glc1-P-Dolichol) LLO in membrane bilayers. Microsecond molecular dynamics simulations of LLOs revealed that most carbohydrate residues interact with the membrane lipid head groups, parallel to the membrane surface, while the PP linkages are within the lipid head group, and the isoprenoid chains are within the bilayer. Overall, there are similarities in the orientations, structure, and dynamics of the eukaryotic, bacterial and archaea LLOs in bilayers. LLOs’ preferred orientation, structure and dynamics provided information for complexation with OSTs, allowing further studies of how these enzymes catalyze the transfer of the oligosaccharide chain to an acceptor protein by OSTs.
ASSESSING THE ADAPTIVE CAPACITY OF HOUSEHOLDS TO CLIMATE CHANGE: A CASE STUDY IN QUANG DIEN DISTRICT, THUA THIEN HUE PROVINCE
Authors: Dung Tran Cong , Minh Đao Duy , Lebailly Philippe
Published: May 2019

Abstract: This study aims to identify the adaptation capacity undertaken by households in response to natural disasters and climate changes (CC). A total of 100 households in two communes including Quang Phuoc and Quang Cong, Quang Dien district were interviewed. The findings indicate that in the last few years, these communes have been badly affected by various types of natural hazards, including typhoons, floods, droughts and, and extremely cold weather. The study demonstrates that the adaptive capacity index in Quang Cong is significantly lower than that in Quang Phuoc (0.50 and 0.52). Also, the current adaptation actions of local households in response to natural disasters and CC have focused on short-term actions only. On the basis of the findings, the study proposes key recommendations to local households in Quang Dien district to effectively mitigate and adapt to natural disasters and CC. The recommendations encompass three groups, namely (i) raising awareness and understanding about CC; (ii) improving the infrastructure system; and (iii) diversifying livelihood strategies to increase income.Keywords: climate change, natural disasters, adaptive capacity, index
A POESIA NAS OBRAS DA ORIGEM:
Authors: Carneiro Márcio Danilo De Carvalho , Leal Izabela Guimarães Guerra
Published: May 2019

O presente artigo se propõe a comentar os resultados obtidos em dois projetos tradutórios distintos, de princípios diversos: a transcriação do primeiro capítulo da Torá – a Bíblia Hebraica – empreendida por Haroldo de Campos, e a tradução de João Ferreira de Almeida, que produziu a primeira versão do texto em português, sendo a variante mais difundida em território nacional. Consideramos suas motivações, o método utilizado e as soluções encontradas ao verter o texto para Português. Procuramos investigar o aspecto literário do texto e discutir conceitos de tradução desenvolvidos por Henri Meschonnic, além de detalhar a teoria tradutória de Haroldo de Campos e a obra em questão, intitulada Bere’shit: A Cena da Origem (CAMPOS, 2000), que respeita a respiração prosódica e a disposição tipográfica da composição, motivada pelo Paideuma concretista – Mallarmé, e.e. cummings, Ezra Pound e James Joyce. Discorremos sobre a amplitude do termo “transcriação” como apresentada por Haroldo para esta obra, que mantém o ritmo da poesia originalmente escrita em hebraico, deixando aparente a abordagem poética do tradutor. Nos propomos a averiguar a reconfiguração (transcriação) do texto original pelo uso de atributos modernistas: verso livre, disposição espacial das palavras, aspectos sonoros e a decomposição de palavras e neologismos. Por último, apresentamos uma comparação didática entre a tradução de Haroldo de Campos e a de João Ferreira de Almeida.
A LITERATURA ALEMÃ DE WOLFGANG MENZEL, RESENHADA POR HEINRICH HEINE:
Authors: Silva Felipe Vale da
Published: May 2019

Die deutsche Literatur von Wolfgang Menzel (1828) não é apenas uma das resenhas mais conhecidas de Heinrich Heine, mas também um importante documento sobre a cultura alemã em sua fase de transição do romantismo para a literatura da Jovem Alemanha (Junges Deutschland). O presente artigo, além de trazer a tradução integral do texto em questão, faz uma leitura que visa a evidenciar as principais tensões vividas por escritores naquele momento de assumida estagnação cultural e censura política. Heine se vale do registro da resenha para propor um programa estético inovador, e o faz declarando o final do Período da Arte (Kunstperiode) representado por Johann Wolfgang von Goethe e os teóricos do romantismo.
STRATEGI PEMERINTAH KOTA MALANG DALAM MENINGKATKAN KUALITAS PELAYANAN PUBLIK BERBASIS KONSEP SMART CITY
Authors: Wanto Alfi Haris
Published: May 2019

Lahirnya konsep smart city tumbuh seiring semakin majunya perkembangan teknologi informasi yang merambah hampir seluruh kalangan, tua muda, kaya miskin, desa kota. Masyarakat semakin mudah dan cepat melakukan komunikasi, mendapatkan informasi yang dibutuhkan, serta melakukan pekerjaan lain dengan lebih mudah, cepat, dan ringkas. Metode penelitian ini menggunakan pendekatan kualitatif dengan jenis penelitian deskriptif. Sumber data yang dipergunakan antara lain adalah Informan, Peristiwa, dan Teknik Pengumpulan Data menggunakan wawancara, observasi dan dokumentasi. Instrumen Penelitian dalam penelitian ini menggunakan peneliti sendiri, pedoman wawancara dan perangkat penunjang seperti buku catatan, alat perekam, dan kamera. Model analisis data yang digunakan peneliti adalah model interaktif Miles, Huberman, dan Saldana (2014: 12-14) meliputi Kondensasi data (data condensation), Penyajian Data (data display) dan Penarikan Kesimpulan (Conclusions drawing). Hasil penelitian ini adalah Pemerintah Kota Malang melakukan beberapa strategi dalam meningkatan kualitas pelayanan publik berbasis konsep smart city antara lain dengan beberapa strategi. Yaitu: Strategi membangun (political environment) yang mendukung terselenggaranya tata kelola pemerintah kota Malang yang lebih melayani. Membangun lingkungan politik, Strategi membangun teknologi yang mendukung terselenggaranya proses pelayanan publik yang berebasis pada perkembangan ilmu pengetahuan dan teknologi yang kini mengalami perkembangan yang sangat pesat. Strategi menyiapakan dukungan anggaran untuk terselenggaranya program tersebut. Strategi partisipasi seluruh stakeholders dalam mendukung terlaksananya program tersebut. Stakeholders di sini bisa berasal dari masyarakat, maupun dunia usaha (private sector), tanpa partisipasi dari seluruh stakeholders tersebut kebijakan yang bagus sekalipun akan terasa kurang berdampak pada perubahan positif yang merupakan dampak (outcome) dari sebuah kebijakan yang dihasilkan oleh (policy maker). Saran dari riset ini adalah pemerintah perlu menyiapkan strategi dalam upaya memastikan program ini terus bisa berjalan dengan baik (sustainable).
PERENCANAAN PENGELOLAAN PENDAPATAN ASLI DAERAH SEKTOR PAJAK BUMI DAN BANGUNAN PERDESAAN DAN PERKOTAAN DI KABUPATEN BLORA
Authors: Nugrahadi Robi , Sarwono Sarwono , Riyanto Riyanto
Published: May 2019

This research background is property tax management authority transfer by center government to local government according to the laws of local tax number 28 in 2009. The purpose is to make government financial independently and increase local revenue in property tax sector. Problem of that condition is local government must be set up many supporting factors, that is human resources especially property appraisal, computer and server tools, operating system and data base, compatibel files and service room, and exist budget to implement intensification and extensification tax program. Readiness of supporting factors are very influence optimization level of property tax revenue. According that condition, this research purpose is to analyse property tax management in Blora Regency so that from result of analysing can be arrange scenario planning which is consider uncertainty situation in future. Collecting data method of this research use Miles, Huberman, and Saldana method (2014), while scenario planning analysis use TAIDA method from Lindgren and Bandhold (2003). Result of this research is Government of Blora Regency has not maximaly implement property tax management yet. That condition based on empirical fact which is only one man appraisal to analyse NJOP with more than 500 thousand property tax object in Blora Regency. That situation cause property tax revenue not accordance with real potension.
IMPLEMENTASI KEBIJAKAN REKTOR TENTANG TATA KRAMA DAN ETIKA MAHASISWA DI UKM/ORMAWA UNIVERSITAS NEGERI PADANG
Authors: Ningrum Nillam Martha Sonia , Khaidir Afriva , Alhadi Zikri
Published: May 2019

This study aims to determine the implementation of Rector's policy on student's etiquette and ethics in Student Activity Units or Student Organization (UKM/Ormawa) Padang State University (UNP) based on Circular Letter Number 3916/UN35/KM/2017. The background of this research because there are still some points in the circular that did not happen like the activities of students until the night, making the secretariat as a place to sleep, and the safety and cleanliness of PKM that has not been awake. This research is a qualitative research using descriptive method. The informant of this research is Vice Rector III UNP as the maker of SE No.3916/UN35/KM/2017, Vice Dean III Faculty of Social Sciences, Kabag. Student Affairs and alumnus, Kasubbag. MPIK's Student Affairs, UKM Coach, Security Guard, BEM and MPM, some UKM/Ormawa managers UNP environment, students and communities living around UNP who can provide information. Techniques and data collection tools in this study using interview techniques, observation and documentation studies. In the data collection used research instruments in the form of interview guides, observation format and documentation study format. In this study the data analyzed obtained from interviews and interpreted qualitatively, in the form of abstraction, words and statements. The result of the research shows that the implementation of Rector's policy on students' etiquette and ethics in UKM/Ormawa UNP is good enough with the form of control that is done related to the etiquette and ethics in the student organization at UNP only still need improvement on the policy that has been implemented. Constraints encountered in the implementation of SE No.3916/UN35/KM/2017, among others, still low understanding and awareness of the contents of the policy, facilities and infrastructure are still inadequate, less assertive sanctions given to violators and still lack of supervision from the Rector and other related parties. From the results of this study the researchers provide advice to the parties involved, including to the Rector of UNP as policy makers maintain communication, coordination and good cooperation with each element, and for the implementation of the policy firmly need a rule of law is stronger than the circular, so also with the students and coaches UKM/Ormawa Coach to always direct and supervise the activities of students to be more directed and orderly. In addition, there must be full security and supervision from the security guard and the community around UNP, as well as students and UKM/Ormawa there must be understanding and awareness of existing regulations to be more sensitive to a policy so that the implementation of Rector policy can run optimally.
POLA FLUKTUASI KEKERUHAN AIR DI AREA POTENSIAL BANJIR SUNGAI CIUJUNG KECAMATAN KRAGILAN, KABUPATEN SERANG
Authors: Prihartanto .
Published: May 2019

ABSTRAKFluktuasi Kekeruhan air Sungai Ciujung di area potensial banjir Kecamatan Kragilan, Kabupaten Serang memiliki pola yang berbeda antara musim kemarau dan musim hujan. Untuk menganalis pola fluktuasi turbiditas di Sungai Ciujung,  maka digunakan data sekunder kualitas air sungai pada musim kemarau 2013 untuk parameter zat padat tersuspensi yang memiliki kontribusi terhadap kekeruhan sungai. Sebagai pembanding dilakukan pengukuran kekeruhan secara langsung pada musim hujan 2016. Dari hasil perbandingan kedua musim tersebut diperoleh perbedaaan pola fluktuasi kekeruhan antara musim kemarau 2013 dan musim hujan 2016. Kegiatan-kegiatan domestik, komersial dan industri memiliki kontribusi terhadap pola fluktuasi kekeruhan di S. Ciujung yang berada di Kecamatan Kragilan.. Di samping itu, sedimentasi terjadi juga sepanjang sungai. Kata Kunci: Kekeruhan, Sedimentatsi, Banjir, Padatan Tersuspensi
SISTEM INFORMASI BENCANA TANAH LONGSOR (SI-BENAR) BERBASIS WEB UNTUK WILAYAH DESA CILILIN, KECAMATAN CILILIN, KABUPATEN BANDUNG BARAT
Authors: Fiqi Bondan , Turyana Iyan , Santoso Eko Widi
Published: May 2019

Tanah longsor merupakan peristiwa kebencanaan yang kerap terjadi di wilayah Desa Cililin, Kecamatan Cililin, Kabupaten Bandung Barat, yang memiliki dampak negatif diantaranya adalah jatuhnya korban jiwa, kerugian rusaknya insfrastruktur, perekonomian yang tersendat, menurunnya harga tanah di daerah setempat serta trauma psikis bagi para korban selamat sehingga menimbulkan berbagai gangguan kejiwaan. Bencana tanah longsor dapat terjadi dimana dan kapan saja, namun dapat diidentifikasi lebih dini menggunakan Early Warning System (EWS) tanah longsor. EWS tanah longsor memerlukan suatu tampilan untuk menampilkan data-data monitoring kepada stakeholder yang berkaitan dengan bencana tanah longsor berupa sebuah aplikasi sistem informasi. Aplikasi sistem informasi bencana tanah longsor (Si-Benar) berbasis web ini dirancang menggunakan beberapa tahapan perancangan desain diagram, desain tampilan, menggunakan bahasa pemrograman PHP, basis data MySQL dan menampilkan data-data sensor dari hardware EWS tanah longsor dengan tampilan responsif. Sistem informasi bencana tanah longsor (Si-Benar) berbasis web merupakan solusi untuk memberikan informasi faktual mengenai data-data yang digunakan sebagai bahan monitoring dan evaluasi mengenai potensi bencana tanah longsor di wilayah Desa Cililin, Kecamatan Cililin, Kabupaten Bandung Barat. Kata kunci: Tanah Longsor, Early Warning System (EWS), Aplikasi sistem informasi bencana tanah longsor (Si-Benar), Data
L2 motivation in ESP and EGP courses: An investigation of L2 motivational selves among learners of English in Saudi Arabia
Authors: Altalib Aser
Published: May 2019

This paper investigates the L2 motivation of Saudi university students in ESP (English for Specific Purposes) and EGP (English for General Purposes) courses. One of the common arguments about ESP courses suggests that they are more likely to generate higher levels of motivation than other types of English courses (i.e., EGP courses). Some scholars (e.g., Basturkmen, 2010; Dudley-Evans & St John, 1998; Hutchinson & Waters, 1987) hold this view, asserting that ESP courses are more relevant to learners’ needs and interests, which increases their motivation. However, none of these claims are based on empirical research; the present study aims to fill this gap. Using Dörnyei’s (2005, 2009) L2 Motivational Self System, 4,043 students enrolled in ESP and EGP courses at four Saudi universities completed an online survey. The analysis showed a significant relationship between learners’ motivation and their attended English course. The ESP group had higher ideal L2 selves and more positive attitudes towards the L2 learning experience than the EGP group, whereas the ought-to L2 selves were not significantly different. In addition, a multiple regression model was designed, and indicated that the two self-constructs had an impact on participants’ L2 achievements, either positively or negatively.
An investigation of learners’ use of CAN and COULD
Authors: Whitty Lauren
Published: May 2019

CAN and COULD have multiple uses and multiple interpretations which can be difficult for learners of English to understand. For example, the difference of Can you help me? and Could you help me? may go unnoticed to an English language learner, but a native speaker of English would recognise a difference in politeness. The current paper reports on an investigation of learners’ use of the modal auxiliaries CAN and COULD, including their negative counterparts (cannot/can’t and could not/couldn’t), in an English Proficiency Program (EPP) classroom at a New Zealand University. Through first examining the learners’ use of CAN and COULD in spoken and written contexts, comparisons are made to their use in the British National Corpus, which results in the identification of areas in which learners’ use could be strengthened.
Integrating multimodal technologies with VARK strategies for learning and teaching EFL presentation: An investigation into learners’ achievements and perceptions of the learning process
Authors: Lee Yow-jyy Joyce
Published: May 2019

Successful oral presentation effectually involves multi-faceted training of listening, writing, and nonverbal delivery besides speaking orally, which calls for a systematical holistic/multimodal approach. However, a multimodal learning environment for fostering EFL learners’ presentation development remains virtually unexplored. This study employed multimodal strategies adopted from the VARK model (visual, aural, reading/writing, and kinesthetic/gestural) with the support of digital audio, video, and speech visualization technologies in an English presentation course at a university in Taiwan. Two EFL classes served respectively as the experimental group with a technology-mediated multimodal approach and the control group with a traditional oral approach. Specifically, this research evaluated the experimental participants’ oral performance and explored their perceptions of this technology-mediated multimodal approach and its advantages and disadvantages as identified by the participants. Results from independent t-tests showed marginal significant progress of presentation performance in the experimental group. Descriptive statistics from the perception survey and content analysis of students’ reflective responses indicated that the participants were overwhelmingly positive about technology-supported multimodal activities implemented in the oral training course but encountered psychological and technological challenges when producing multimodal assignments. Theoretically, the results support the extension of multimodal theory to EFL oral presentation education. Practically the study informs EFL presentation instructors of the validity of technological-enhanced VARK strategies for learning and teaching EFL presentation. The research results also bear significant implications for the necessity of learner training on technology practices when pedagogy with the integration of multimodal technologies into EFL speech education is implemented.
Effect of immunity inductors on the structural and functional state of photosynthetic apparatus and oxidative status of cucumber plants (cucumis sativus l.) Infected by Fusarium oxysporum
Authors: Аbramchik L. M. , Domanskaya I. N. , Makarov V. N. , Serdiuchenko E. V. , Bachyshcha T. S. , Коndratieva V. V. , Shpilevski S. N. , Daubniuk Yu. N. , Каbashnikova L. F.
Published: May 2019

The effect of inductors of the immune response of β-aminobutyric acid (BABA), β-1.3-glucan (GK), salicylic acid (SA) and their mixture on the structural and functional state of photosynthetic apparatus and the oxidative status of cucumber plants under infection by fungal pathogen Fusarium oxysporum was studied. It was found that the disorganizing effect of the pathogenic fungus Fusarium oxusporum in cucumber plants caused the suppression of the synthesis of photosynthetic pigments and the functional activity of the photosystem 2 in chloroplast membranes, as well as in changing the character of the redistribution of absorbed light energy, leading to a decrease in the photochemical energy conversion (qP) and enhancing non-photochemical quenching (qN) of chlorophyll. In this conditions intensification of lipid peroxidation processes was observed. The use of immunomodulators such as BABA, GK and SA promotes the improvement of the adaptive properties of the photosynthetic apparatus, and the reduction of oxidative processes activity in infected cucumber leaves, which indicates the protective role of these substances against the fusarious wilt caused by Fusarium oxysporum.
Composition of essential oil of plants some species of the genus Agastache l. Introduced in Belarus
Authors: Kovalenko N. A. , Supichenko G. N. , Leontiev V. N. , Shutova A. G.
Published: May 2019

The steam distillation method was used to obtain samples of essential oil of some species of genus Agastache L. introduced in Belarus. More than 20 components have been identified and determined in the essential oils of Agastache pallidiflora ssp.neomexicana L., Agastache aurantiaca L., Agastache mexicana L., Agastache foeniculum L. by gas liquid chromatography.

The quantitative composition of the samples of essential oils depends on the type of plant material. The main its components are limonene, 1.8-cineol, mentone, isomentone, methyl chavicol, methyeugenol. The main component of essential oils Agastache pallidiflora ssp. neomexicana L. и Agastache aurantiaca L is mentone (40–65 %). Essential oil Agastache foeniculum L. is enriched with pulegone and isomentone. Methyl chavicol and methyleugenol dominate in Agastache mexicana essential oil. Distribution of the enantiomers of limonene, menthone and pulegone are shown. Regardless of the plant species, the samples are optically pure in the (+)-pulegone. The nature of the distribution of the enantiomers of limonene and menthone is an individual characteristic of the studied Agastache L. essential oils.
Morphological changes of mitochondria and mammalian cells, induced by hypochlorous acid
Authors: Zavodnik I. B. , Kravchuk R. I. , Ilyich T. V. , Lapshina E. A. , Vejko A. G. , Zavodnik L. B. , Astrowskaja O. B. , Kurbat M. N.
Published: May 2019

Hypochlorous acid, HOCl, is one of the most powerful biological oxidants and the most important mediator of inflammatory damage of cells and tissues. The purpose of this study was to characterize the morphological features of HOCl – induced oxidative impairment in rat liver mitochondria in vitro and to compare the processes of HOCl-induced oxidation in mitochondria, erythrocytes and B14 cells.

HOCl addition (300 μM) to mitochondrial suspension resulted in mitochondrial structural changes with a decrease in the mean total length of the crista and the average number of cristae in one mitochondria with no change in the length of one crista. There was shown a slight decrease in the average cross-sectional area of one mitochondria, mitochondrial profile elongation, an increase in the number of altered mitochondria and the heterogeneity of the population. Simultaneously we observed depolarization of the mitochondrial membrane, the rate and degree of which were determined by the concentration of HOCl. HOCl addition (25–150 μМ) induced lysis of erythrocytes for 60–180 s, which was preceded by a change in the shape and size of cells. The apparent dissociation constant for the HOCl – membrane complex was estimated to be Kd = 140 ± 25 μМ, and the Hill coefficient was to be 2.1. The B14 cell exposure to HOCl (100 μМ) led to a loss of ability to sorb on the substrate, to form associates, and to subsequent shrinkage of cells.

Therefore, HOCl caused some morphological (and functional) changes in rat liver mitochondria, which may serve as one of the causes of cell death in inflammatory foci. At the level of the whole cells, the HOCl addition induced lysis of red blood cells and deep damage to B14 cells.
Protein accumulation and expression of the nitrate reductase gene in Spirulina platensiscells depending on the spectral composition of led radiation
Authors: Kozel N. V. , Radyuk M. S. , Samovich T. V. , Dremuk I. A. , Gabrielyan L. S.
Published: May 2019

The effect of LED lighting of different spectral composition on the productivity of Spirulina platensis, an accumulation of protein in alga cells and an expression of the nitrate reductase gene has been studied. It was shown that LED lighting with a predominance of the red component in the emission spectrum allows achieving 9–29 % higher alga productivity compared to using fluorescent lamp illumination. Illumination with single blue light resulted in significant (83 %) decrease in the productivity of Spirulina platensiswhich apparently was due to the absence of the yellow and red components in the illuminator spectral composition, which are most effectively absorbed by phycocyanin. A positive correlation between an increase in the productivity of alga and the accumulation of protein in its cells was found. So, by using an illuminator with red LEDs, the protein content increased by 21 % calculated per gram of dry weight and 47 % calculated per liter of suspension relative to the control. Analysis of the expression of the Nar gene encoding nitrate reductase in Spirulina platensiscells did not reveal a direct dependence between an increasing protein accumulation and an expression level of the Nar gene in the most promising in terms of biomass and protein yield sample of alga, growing under red LEDs. This indicates the crucial role of the photosynthetic activity of Spirulina platensiscells in increasing productivity and protein synthesis.
Growth features of grafted plants of Juniperus Chinensis‘Blaauw’
Authors: Kelko H. F.
Published: May 2019

Juniperus chinensis‘Blaauw’ is difficult to propagate by rooting stem cuttings why the aim of the research was assessing the possibility of obtaining the progeny of this cultivar by grafting. Experiments conducted in 2012 on grafting with various methods of the cultivar on 2-year-old Juniperus virginiana‘Grey Owl’ seedlings of rooted cuttings origin have made it possible to achieve a survival rate of about 80 %, however, the final conclusions on the success of transplantation can be made only on the basis of further long-term observations of grafted plants. It is shown that the plants of the Juniperus chinensis ‘Blaauw’, grafted by veneer side grafting, are more viable than the plants grafted by side grafting. During the observation period 2012–2018 years in the latter case, 75 % of the grafted seedlings died, and only 38.5 % of the veneer side grafted plants fell out. Grafted seedlings are characterized by more powerful growth in comparison with seedlings of rooted cuttings origin. The annual increment of the first is 2–2.5 times higher, which is explained by the influence of the stock. The site of grafting becomes difficult to discern over time, which indicates a good compatibility of grafted components and a suitable way of carrying it out. Thus, veneer side grafting is a promising way to multiply the hard-to-root Juniperus chinensis‘Blaauw’.
DNA binding properties of 2ʹ-hydroxyflavanon and Schiff base derivative
Authors: Korolevich V. M. , Blazinska P. , Sykula A. , Lodyga-Chruscinska E.
Published: May 2019

Flavanoids a class of plant and fungus secondary metabolites. 2ʹ-Hydroxyflavanone was previously isolated from Mimosa pudica (L.) whole plant and was found to exhibit anti-inflammatory effects in vitro and binding with calf timus DNA. There are also reports on anti-inflammatory properties of compounds bearing flavanone/chromone nucleus. The aim of this work was to develop a synthesis of new azomethine compounds derived from flavanones, to examine their spectroscopic properties and interaction with DNA. 2ʹ-Hydroxyflavanone and thiocarbohydrazide were used as substrates in the synthesis. The obtained products were analyzed by 1H NMR spectroscopy, UVVis. Ultraviolet spectroscopy was used to analyze the chemical-physical properties. Mechanism of interaction of bioactive 2ʹ-hydroxyflavanone with calf thymus deoxyribonucleic acid (DNA) was studied employing UV absorption. 2ʹ-Hydroxyflavanon and 2ʹHFTCH are photostable in DMSO. The interaction of 2ʹ-hydroxyflavanone and its derivative occurs by the mechanism of intercalation. The change in the structure of the 2ʹ-hydroxyflavanone molecule by Schiff base modification leads to an increase in DNA-binding properties. High binding ability of 2ʹ-hydroxyflavanone with DNA may be useful for development of new anti-inflammatory and antimicrobial remedies.
Establishing sustainable roadside vegetation communities of high botanical and aesthetic value: the basic concept statement and experimental justification
Authors: Voznyachuk I. P. , Vlasava N. B. , Stepanovich I. M. , Godneva A. T. , Reshetnikov V. N.
Published: May 2019

The basic principles for the development of a new greening system for the region of Belarus aimed at creation and restoration of sustainable roadside plant communities of high botanical and aesthetic value are formulated on the basis of the use of conservation, restoration and “incorporation” methods of flowering native plant species, characteristic of a particular region. The statements of the concept are developed and recommended for roadways and highways in the Republic of Belarus and tested on the model site between the city of Myadel and the resort village of Naroch of Highway P28. Roadside vegetation management practices in other countries as well as our research has shown that along the investigated roads there is a high adaptive potential of native flora; by changing the mowing regime, sustainable plant communities can be established while enhancing the aesthetics and expressiveness of key elements of the landscape of the roadsides. The implementation of the strategy for each geobotanical region depends on the development of an assortment of native plant species that can be used for the greening of roadside areas. This should be done by taking into account their geobotanical and edaphic features, creation of a seed bank for grass mixtures, the formation of natural genetic reserves (field banks) of meadow flora to conserve the local and overall ecosystem and genetic balance. For examples, the atlas of plants “Roadside flowers” developed for the National Park “Narochansky” is recommended for use in roadsides (verges) greening within the boundaries of the Oshmyany– Minsk geobotanical district. To expand the use of gained positive experience on increasing the biodiversity of roadside ecosystems and reducing the cost of their maintenance, it is desirable to implement this strategy for the entire road network of Belarus, which will require adjustment in technologies for managing roadside ecosystems, based on priorities of conservation, restoration and formation of native plant communities, including meadows and wetlands.
Generalizing Khinchin’s theorem to a linear combination of analytical linearly independent functions
Authors: Bernik V. I. , Budarina V. N. , O’Donnell H.
Published: May 2019

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Solving the mixed problem for the Klein–Gordon–Fock type equation with integral conditions in the case of the inhomogeneous matching conditions
Authors: Korzyuk V. I. , Stolyarchuk I. I.
Published: May 2019

The classical solution of the mixed problem with integral conditions for the Klein–Gordon–Fock type equation in the half strip is considered when inhomogeneous matching conditions are fulfilled. An equivalent conjugation problem is formulated where conjugation conditions are set on characteristics. Constructed inhomogeneous conditions uniquely define gaps of the solution or its derivatives on characteristics and given gaps can be either remained or smoothed while the time argument increases depending on the kernel of the integral operator in unlocal conditions. The solution of this problem is reduced to solving the second-type Volterra integral equations and their systems. The unique solution of these equations in the class of the twice continuously differentiable functions exists when the initial functions are smooth enough. While considering the given problem the method of characteristics is used to construct both an analytical solution, when the solution of the integral equation can be found explicitly, and an approximate solution. Moreover, approximate solutions can be constructed in numerical and analytical form. When the numerical solution is constructed, matching conditions are significant and need to be considered while developing numerical methods.
Forming immobilized nanostructured titanium dioxide having antibacterial properties
Authors: Baglov A. V. , Shevtsova T. A. , Khoroshko L. S. , Dudchik N. V. , Drоzdova A. V. , Borisenko V. E.
Published: May 2019

Nanostructured titanium dioxide (TiO2) coatings were formed on rigid aluminum and flexible fiberglass substrates using the sol-gel method modified by adding nano- and submicron TiO2 particles to the sol. The use of sols with such particles leads to the formation of a rough coating of agglomerates of nano- and submicron particles bonded with sol-gel synthesized TiO2. The antibacterial activity of the coatings was confirmed under irradiation with visible light from an LED source for 120 minutes. The most activity was demonstrated by all samples in relation to Staphylococcus aureus.
Distribution of ammonia, mono-, di- and triethylamine in the water solution–gas phase system.
Authors: Kosandrovich E. G. , Shachenkova L. N. , Soldatov V. S.
Published: May 2019

The equilibrium distribution of ammonia, ethylamine, diethylamine and triethylamine between their aqueous solutions and the gas phase at 25 ± 0.1 °C have been investigated under equilibrium conditions. Based on the results obtained (using the equations of Henry’s law and the ionization constant), Henry’s constants for these substances have been calculated with the consideration of their interaction with water. It was established that Henry’s law is valid, at least, in the range of amine concentrations in the air 0–200 mg/m3.
Photoisomerization and photoorientation of brilliant yellow in the thin film state
Authors: Lemiashonak S. N. , Chaplanova Zh. D. , Agabekov V. E. , Ivanova N. A. , Kukhta I. N.
Published: May 2019

The features of photoisomerization and photoorientation of azo dye brilliant yellow (BY) in the thin film state are studied. The trans-cis-isomerization of the dye molecules is established to proceed upon irradiation of the films based on BY with a full light of an ultra-high pressure lamp DRT-1000 (UV / VID light), this reaction is described by the first-order reaction equation. Under the action of linearly polarized blue light (λ = 457 nm), an optical anisotropy arises in films of BY. Optically anisotropic films of BY with a high dichroic ratio (>10) are shown to be formed using N-methylpyrrolidone and N, N-dimethylformamide as solvents and cellulose acetate as the polymer matrix.
Screening, characterization and molecular-genetical identification of a new bacterial strain Paenibacillus species
Authors: Sapunova L. I. , Lobanok A. G. , Yatsevich K. K. , Kulish S. A. , Tamkovich I. A. , Yarkhova L. V. , Sysaliatsin Ya. M.
Published: May 2019

Bacterial variant PS-K-17 was isolated from wheat grain contaminated by polysaccharide-producing microbiota for further characterization. It was found that the isolate grown on agar slants and in submerged culture on media with specific substrates synthesized beta-galactosidase, amylase, protease, pectinase, cellulase, beta-glucanase, lipase (esterase), alginase, extracellular polysaccharides, and pigments, probably carotenoids. Based on cultural-morphological and physiological-biochemical properties and phylogenetic analysis of nucleotide sequences of 16S rRNA gene (access code MF443394 in GenBank) the bacterial culture was identified as Paenibacillus species PS-K-17. The studied isolate forms one phylogenetic branch with type strains Paenibacillus nicotianae (98.3 %), Paenibacillus hordei (98.2 %), Paenibacillus kyungheensis (97.9 %), holding wherein a separate position. Strain Paenibacillus sp. PS-K-17 may find use in biotechnology as a producer of extracellular polysaccharides and enzymes splitting plant polymeric substances as well as a component of microbial consortium-ingredient of a new complex feed additive.
Physicochemical properties of lactoferrin under oxidative/halogenative stress
Authors: Terekhova M. S. , Grigorieva D. V. , Gorudko I. V. , Semak I. V. , Sokolov A. V. , Panasenko O. M. , Cherenkevich S. N.
Published: May 2019

Lactoferrin (Lf) was discovered in the thirties of the twentieth century. Since that time a number of useful properties of Lf (antibacterial, antiviral, pro- and anti-inflammatory, etc.) have been found. That’s why Lf became a promising candidate for pharmaceuticals use. The concentration of Lf strikingly increases in inflammatory focuses due to neutrophil degranulation. At the same time, activated neutrophils starts to generate reactive oxygen and halogen species (ROS and RHS), which leads to the development of oxidative/halogenative stress. In this work, using the fluorescence analysis we found the change of the Lf structure and properties in the inflammation conditions (under oxidatives/halogenative stress). We use two forms of Lf – human Lf, excreted from human milk, and recombinant Lf, excreted from milk of transgenic goats. It was established that the amino acids of Lf (decreasing the number of tryptophanils and primary amines) and protein restructuring undergo modification under the HOCl action, while H2O2 has no influence. These changes in the molecule under the HOCl treatment result in decreasing the iron-binding capacity of Lf.
Long-term effects of electromagnetic radiation of the mobile phone frequency on the morphofunctional state of the reproductive system of rats and males and their offspring
Authors: Chueshova N. V. , Vismont F. I.
Published: May 2019

The experiment established the long-term effects of low-intensity electromagnetic radiation from a mobile phone (EMR MP 1745 MHz, xpd = 7.5 ± 0.3 μW/cm2) in the offspring of male rats from parents, one of which was either subjected to a prolonged exposure of the investigated factor. A change in the sex ratio of the born offspring and a decrease in their body weight have been established. The analysis of the reproductive system of the offspring of males when they reached the age of 2 and 4 months, revealed significant impairments, with the most pronounced changes found in the offspring obtained from both the irradiated parents or when only the male was irradiated. These changes are manifested in the disruption of the normal functioning of the spermatogenic epithelium, namely, an intensification of the initial stage of spermatogenesis is noted, with a significant inhibition at the stage of spermatid transformation. A decrease in the number of mature germ cells – spermatozoa and a pronounced deterioration of their viability, as well as an increase in testosterone secretion is established. The revealed violations in the morphofunctional state of the reproductive system of the offspring suggest that the trans-generation effect of EMR exposure generated by cellular sources can be considered as a factor affecting the decline in the male fertility.
Effect suppresive therapy with levothyroxine on the state of the cardiovascular and sympathoadrenal systems
Authors: Mityukova T. A. , Leonova T. A. , Kohan S. B. , Sivakov A. A. , Polulyach O. E.
Published: May 2019

Thyroid hormones can affect the cardiovascular system directly by binding to nuclear thyroid receptors, as well as indirectly by changing the neuro – humoral status of the body as a whole. The aim of the work was to assess the effect of levothyroxine suppressive therapy on the regulation of the heart rate and activity of the sympathoadrenal system, as well as the level of cortisol in the blood of young patients with thyroid cancer. It is shown that the effect of suppressive therapy with levothyroxine on the state of the cardiovascular system is manifested in an increase in the risk of tachycardia and changes in heart rate variability associated with the body mass index. Patients with thyroid cancer had a sharp decrease in normetanephrine (norepinephrine metabolite) in the urine. The levels of metanephrine (metabolite of adrenaline) and dopamine in the urine was dependent on the tonus of the autonomic nervous system and blood pressure. The revealed shifts indicate that against the background of subclinical hyperthyroidism, the mechanisms aimed at stabilizing the heart rate and preventing the effects of hyperstimulation of thyroid and beta-adrenoreceptors of the myocardium are included.
Ultradispersed hardening of metal-ceramic microcomposites and coatings containing borides of transition metals in a low-temperature plasma flow
Authors: Rudenskaya N. A.
Published: May 2019

The results of extended researches of processes of modification of morphology, composition and structure of polydispersed refractory powders and microcomposites based on CrB2, TiB2, TiCrB2, Al2O3 in the flow of low-temperature plasma and its use for the formation of plasma coatings – layered composites are presented.
Integration policy at the subnational level in the EAEU: the main aspects of formation
Authors: Vertinskaya T. S.
Published: May 2019

The necessity to form the integration policy at the subnational level to the existing mechanism of economic integration in the EAEU is shown. The content of the conceptual framework of its elaboration, the features of the object and the subnational level of management as a subject of the policy is disclosed. Taking into account the existing regional restrictions, the directions of the integration policy are justified, including the regional policy aligned at the level of the EAEU member countries, the institutional framework, the measures to deepen interregional and border relations based on the implementation of regional reserves and a closer linkage with the regional development.
New highly productive plant type of pigs “Pridneprovsky” in the Belarusian meat breed
Authors: Sheyko I. P. , Sheyko R. I. , Yanovich E. A. , Pristupa N. V. , Timoshenko T. N. , Zayats V. N. , Anihovskaya I. V. , Kapshevich K. A.
Published: May 2019

As a result of the research conducted, it was determined that the performance indicators of created plant type correspond to the indicators of the target standard. Performance of first litter gilts, as well as sows with two or more farrowings, amounted to 11.8 and 11.9 piglets of multiple pregnancy rate, 54.5–55.2 kg of milk yield, for the number of piglets and litter weight at weaning – 10 animals, 84.3–85.1 kg, respectively. Among the selected boars for reproduction according to a greater part of traits, animals of Zaslon line were the best, with indicators of the age of reaching a live weight of 100 kg and an average daily weight gain from birth to 100 kg of live weight made 170.5 days and 717 g, respectively. During the lifetime assessment of meat traits in young animals of the created type selected for self-repair, the back fat thickness values measured at two points of the back, as well as the height of the longest muscle and the lean meat content in body made 16.0, 18.7 and 44.2 mm and 52.0 %, for gilts, respectively, and 15.7, 17.2 and 45.3 mm and 53.2 % for boars, respectively. Calculation of the complex index of sows showed that in most animals its value made 100 points. Assessment of the genetic structure of animals of the new plant type in the Belarusian meat breed by genes RYR 1, ESR, H-FABP and IGF2 was carried out.
Scientific bases of the control system improvement of material and monetary resources by agricultural production
Authors: Makrak S. V.
Published: May 2019

Gaining gross production in all the sectors of the national economy in short term is constrained by the limited available resource potential, as well material and cash resources. Level of material and cash costs predetermines the competitiveness of products as well in both domestic and foreign markets. In this regard, issue of efficient management system for  material resources, especially for agricultural sector, is becoming increasingly vital. It happens due to the fact that aggregate gross production level in this industry should have a positive trend of growth in order to achieve food security and increase export potential of the national economy. At the same time, management of material resources in agriculture has its own peculiarities, and management efficiency is predetermined in particular by the best combination of mineral fertilizers, plant protection products and other resources combined with the production and economic potential of a particular region or agricultural companies. The paper dwells on systematic analysis of material and cash costs in agricultural production, as well as for branches of arable farming and livestock breeding; particular attention is paid to the natural consumption of material resources under the nomenclature as a key factor determining the competitiveness of agricultural products. The main directions for improving the material resources management system for three levels (macro, micro and nano) have been substantiated, specific tools have been developed for implementing these directions, methodological implementation approaches have been proposed for each direction. Algorithm was developed and tested to substantiate the criteria for rational use of material and cash resources and to increase efficiency of agriculture. Factors (sectoral, regional and internal) are proposed having primary impact on the efficient consumption of material resources, taking into account the conditions of location and specialization of crop production. Method for assessing attractiveness of agro-resources supplier in conditions digital economy development has been proposed as an effective tool for managing material resources based on marketing approaches. The above mentioned developments can be used for drawing up regional development programs, business plans of agricultural organizations, for express analysis of suppliers at tenders for procurement of material resources for agriculture.