bluuebunny/crossref_metadata_2025 huggingface dataset:
Miniseries 2—Septal and paraseptal accessory pathways—Part II: Para-Hisian accessory pathways—so-called anteroseptal pathways revisited
Authors: Farré Jerónimo , Anderson Robert H , Sánchez-Quintana Damián , Mori Shumpei , Rubio José-Manuel , García-Talavera Camila , Bansal Raghav , Lokhandwala Yash , Cabrera José-Angel , Wellens Hein J J , Sternick Eduardo Back
Published: November 2021

Surgeons, when dividing bypass tracts adjacent to the His bundle, considered them to be ‘anteroseptal’. The area was subsequently recognized to be superior and paraseptal, although this description is not entirely accurate anatomically, and conveys little about the potential risk during catheter interventions. We now describe the area as being para-Hisian, and it harbours two types of accessory pathways. The first variant crosses the membranous septum to insert into the muscular ventricular septum without exiting the heart, and hence being truly septal. The second variant inserts distally in the paraseptal components of the supraventricular crest, and consequently is crestal. The site of ventricular insertion determines the electrocardiographic expression of pre-excitation during sinus rhythm, with the two types producing distinct patterns. In both instances, the QRS and the delta wave are positive in leads I, II, and aVF. In crestal pathways, however, the QRS is ≥ 140 ms, and exhibits an rS configuration in V1–2. The delta wave in V1–2 precedes by 20–50 ms the apparent onset of the QRS in I, II, III, and aVF. In the true septal pathways, the QRS complex occupies ∼120 ms, presenting a QS, W-shaped, morphology in V1–2. The delta wave has a simultaneous onset in all leads. Our proposed terminology facilitates the understanding of the electrocardiographic manifestations of both types of para-Hisian pathways during pre-excitation and orthodromic tachycardia, and informs on the level of risk during catheter ablation.
Miniseries 2—Septal and paraseptal accessory pathways—Part IV: Inferior paraseptal accessory pathways—lessons from surgical and catheter ablation
Authors: Farré Jerónimo , Anderson Robert H , Rubio José-Manuel , Tretter Justin T , Sánchez-Quintana Damián , García-Talavera Camila , Bansal Raghav , Lokhandwala Yash Yahya , Cabrera José-Angel , Wellens Hein J J , Back Sternick Eduardo
Published: November 2021

Surgeons and electrophysiologists performing accessory pathway ablation procedures have used the term ‘posteroseptal’ region. This area, however, is neither septal nor posterior, but paraseptal and inferior; paraseptal because it includes the fibro-adipose tissues filling the pyramidal space and not the muscular septum itself and inferior because it is part of the heart adjacent to the diaphragm. It should properly be described, therefore, as being inferior and paraseptal. Pathways in this region can be ablated at three areas, which we term right inferior, mid-inferior, and left inferior paraseptal. The right- and left inferior paraseptal pathways connect the right and left atrial vestibules with the right and left paraseptal segments of the parietal ventricular walls. The mid-inferior paraseptal pathways take a subepicardial course from the myocardial sleeves surrounding the coronary sinus and its tributaries. Our review addresses the evolution of the anatomical concept of the inferior paraseptal region derived from surgical and catheter ablation procedures. We also highlight the limitations of the 12-lead electrocardiogram in identifying, without catheter electrode mapping, which are the pathways that can be ablated without a coronary sinus, or left heart approach.
Genetic characterization of ESBL/pAmpC-producing Escherichia coli isolated from forest, urban park and cereal culture soils
Authors: Benlabidi Saloua , Raddaoui Anis , Achour Wafa , Hassen Bilel , Torres Carmen , Abbassi Mohamed Salah , Ghrairi Taoufik
Published: November 2021

Little is known about the role of forestland and non-fertilized agriculture soils as reservoirs of extended-spectrum beta-lactamase (ESBL) and plasmid-borne AmpC (pAmpC)-producing Escherichia coli isolates. Thus, in the present study, 210 soil samples from various origins (forest of Oued Zen (Ain Drahem), non-agriculture soils from different park gardens in Tunis City, cereal culture soils and home gardens) were investigated to characterize cefotaxime-resistant E. coli isolates. A total of 22 ESBL/pAmpC-producing E. coli were collected, and all harbored variants of the blaCTX-M gene (15 blaCTX-M-1, 5 blaCTX-M-55 and 2 blaCTX-M-15). A total of seven and two isolates harbored also blaEBC and blaDHA-like genes, respectively. Resistances to tetracycline, sulfonamides and fluoroquinolones were encoded by tetA (n = 4)/tetB (n = 12), sul1 (n = 17)/sul2 (n = 19) and aac(6’)-Ib-cr (n = 2)/qnrA (n = 1)/qnrS (n = 1) genes, respectively. A total of seven isolates were able to transfer by conjugation cefotaxime-resistance in association or not with other resistance markers. PFGE showed that ten and two isolates were clonally related (pulsotypes P1 and P2). The 10 P1 isolates had been collected from forestland, cereal culture soils and an urban park garden in Tunis City, arguing for a large spread of clonal strains. Our findings highlight the occurrence of ESBL/pAmpC-E. coli isolates in soils under limited anthropogenic activities and the predominance of CTX-M enzymes that are largely disseminated in E. coli from humans and animals in Tunisia.
Temporal and spatial transcriptomic dynamics across brain development in Xenopus laevis tadpoles
Authors: Ta Aaron C , Huang Lin-Chien , McKeown Caroline R , Bestman Jennifer E , Van Keuren-Jensen Kendall , Cline Hollis T
Published: November 2021

Amphibian metamorphosis is a transitional period that involves significant changes in the cell-type populations and biological processes occurring in the brain. Analysis of gene expression dynamics during this process may provide insight into the molecular events underlying these changes. We conducted differential gene expression analyses of the developing Xenopus laevis tadpole brain during this period in two ways: first, over stages of the development in the midbrain and, second, across regions of the brain at a single developmental stage. We found that genes pertaining to positive regulation of neural progenitor cell proliferation as well as known progenitor cell markers were upregulated in the midbrain prior to metamorphic climax; concurrently, expression of cell cycle timing regulators decreased across this period, supporting the notion that cell cycle lengthening contributes to a decrease in proliferation by the end of metamorphosis. We also found that at the start of metamorphosis, neural progenitor populations appeared to be similar across the fore-, mid-, and hindbrain regions. Genes pertaining to negative regulation of differentiation were upregulated in the spinal cord compared to the rest of the brain, however, suggesting that different programs may regulate neurogenesis there. Finally, we found that regulation of biological processes like cell fate commitment and synaptic signaling follow similar trajectories in the brain across early tadpole metamorphosis and mid- to late-embryonic mouse development. By comparing expression across both temporal and spatial conditions, we have been able to illuminate cell-type and biological pathway dynamics in the brain during metamorphosis.
Development of the Wheat Practical Haplotype Graph database as a resource for genotyping data storage and genotype imputation
Authors: Jordan Katherine W , Bradbury Peter J , Miller Zachary R , Nyine Moses , He Fei , Fraser Max , Anderson Jim , Mason Esten , Katz Andrew , Pearce Stephen , Carter Arron H , Prather Samuel , Pumphrey Michael , Chen Jianli , Cook Jason , Liu Shuyu , Rudd Jackie C , Wang Zhen , Chu Chenggen , Ibrahim Amir M H , Turkus Jonathan , Olson Eric , Nagarajan Ragupathi , Carver Brett , Yan Liuling , Taagen Ellie , Sorrells Mark , Ward Brian , Ren Jie , Akhunova Alina , Bai Guihua , Bowden Robert , Fiedler Jason , Faris Justin , Dubcovsky Jorge , Guttieri Mary , Brown-Guedira Gina , Buckler Ed , Jannink Jean-Luc , Akhunov Eduard D
Published: November 2021

To improve the efficiency of high-density genotype data storage and imputation in bread wheat (Triticum aestivum L.), we applied the Practical Haplotype Graph (PHG) tool. The Wheat PHG database was built using whole-exome capture sequencing data from a diverse set of 65 wheat accessions. Population haplotypes were inferred for the reference genome intervals defined by the boundaries of the high-quality gene models. Missing genotypes in the inference panels, composed of wheat cultivars or recombinant inbred lines genotyped by exome capture, genotyping-by-sequencing (GBS), or whole-genome skim-seq sequencing approaches, were imputed using the Wheat PHG database. Though imputation accuracy varied depending on the method of sequencing and coverage depth, we found 92% imputation accuracy with 0.01× sequence coverage, which was slightly lower than the accuracy obtained using the 0.5× sequence coverage (96.6%). Compared to Beagle, on average, PHG imputation was ∼3.5% (P-value < 2 × 10−14) more accurate, and showed 27% higher accuracy at imputing a rare haplotype introgressed from a wild relative into wheat. We found reduced accuracy of imputation with independent 2× GBS data (88.6%), which increases to 89.2% with the inclusion of parental haplotypes in the database. The accuracy reduction with GBS is likely associated with the small overlap between GBS markers and the exome capture dataset, which was used for constructing PHG. The highest imputation accuracy was obtained with exome capture for the wheat D genome, which also showed the highest levels of linkage disequilibrium and proportion of identity-by-descent regions among accessions in the PHG database. We demonstrate that genetic mapping based on genotypes imputed using PHG identifies SNPs with a broader range of effect sizes that together explain a higher proportion of genetic variance for heading date and meiotic crossover rate compared to previous studies.
Development of community strategies supporting brief alcohol advice in three Latin American countries: a protocol
Authors: Solovei Adriana , Mercken Liesbeth , Jané-Llopis Eva , Bustamante Inés , Evers Silvia , Gual Antoni , Medina Perla , Mejía-Trujillo Juliana , Natera-Rey Guillermina , O’Donnell Amy , Pérez-Gómez Augusto , Piazza Marina , de Vries Hein , Anderson Peter
Published: November 2021

Brief alcohol advice offered to patients was shown to be a clinically- and cost-effective intervention to prevent and manage alcohol-related health harm. However, this intervention is not yet optimally implemented in practice. A suggested strategy to improve the implementation of brief alcohol advice is through community actions which would enhance the environment in which primary healthcare providers must deliver the intervention. However, there has been scarce research conducted to date regarding which community actions have most influence on the adoption and implementation of brief alcohol advice. The current protocol presents the development of a package of community actions to be implemented in three Latin American municipalities, in Colombia, Mexico and Peru. The community actions were based on the Institute for Health Care Improvement’s framework for going to full scale, and include: (i) involvement of a Community Advisory Board, (ii) involvement of a project champion, (iii) adoption mechanisms, (iv) support systems and (v) a communication campaign. By presenting a protocol for developing community actions with input from local stakeholders, this article contributes to advancing the public health field of alcohol prevention by potentially stimulating the sustainable adoption and implementation of brief alcohol advice in routine practice.
Association of PLXND1 with a novel subtype of anomalous pulmonary venous return
Authors: Zhou Wei-Zhen , Zeng Ziyi , Shen Huayan , Chen Wen , Li Tianjiao , Ma Baihui , Sun Yang , Yang Fangfang , Zhang Yujing , Li Wenke , Han Bianmei , Liu Xuewen , Yuan Meng , Zhang Guangxin , Yang Yang , Liu Xiaoshuang , Pang Kun-Jing , Li Shou-Jun , Zhou Zhou
Published: November 2021

Anomalous pulmonary venous return (APVR) is a potentially lethal congenital heart disease. Elucidating the genetic etiology is crucial for understanding its pathogenesis and improving clinical practice, whereas its genetic basis remains largely unknown because of complex genetic etiology. We thus performed whole-exome sequencing for 144 APVR patients and 1636 healthy controls and report a comprehensive atlas of APVR-related rare genetic variants. Novel singleton, loss-of-function and deleterious missense variants (DVars) were enriched in patients, particularly for genes highly expressed in the developing human heart at the critical time point for pulmonary veins draining into the left atrium. Notably, PLXND1, encoding a receptor for semaphorins, represents a strong candidate gene of APVR (adjusted P = 1.1e-03, odds ratio: 10.9–69.3), accounting for 4.17% of APVR. We further validated this finding in an independent cohort consisting of 82 case–control pairs. In these two cohorts, eight DVars were identified in different patients, which convergently disrupt the GTPase-activating protein-related domain of PLXND1. All variant carriers displayed strikingly similar clinical features, in that all anomalous drainage of pulmonary vein(s) occurred on the right side and incorrectly connected to the right atrium, which may represent a novel subtype of APVR for molecular diagnosis. Studies in Plxnd1 knockout mice further revealed the effects of PLXND1 deficiency on severe heart and lung defects and cellular abnormalities related to APVR such as abnormal migration and vascular formation of vascular endothelial cells. These findings indicate the important role of PLXND1 in APVR pathogenesis, providing novel insights into the genetic etiology and molecular subtyping for APVR.
Translating Results from VARSITY to Real World: Adalimumab vs Vedolizumab as First-line Biological in Moderate to Severe IBD
Authors: Moens Annick , Verstockt Bram , Alsoud Dahham , Sabino João , Ferrante Marc , Vermeire Séverine
Published: November 2021

Background

Selecting a first-line therapy remains challenging in IBD. Adalimumab (ADM) and vedolizumab (VDZ) effectively lead to endoscopic remission in moderate to severe IBD. The VARSITY trial showed superiority of VDZ over ADM in ulcerative colitis (UC) regarding clinical remission and endoscopic improvement at week 52. We explored these results in a real-world setting of UC and Crohn’s disease (CD).

Methods

This retrospective study followed a cohort of biologic-naïve patients starting ADM or VDZ from 2015-2019. Patients had moderate to severe disease (endoscopic Mayo score ≥2 for UC, presence of ulcerations for CD) prior to therapy initiation. For UC, endoscopic remission (endoscopic Mayo score 0) and improvement (endoscopic Mayo score ≤1) at week 52 were assessed. For CD, endoscopic remission (absence of ulcerations) and improvement (markedly better endoscopy despite ulcerations) at weeks 26-52 were studied. Treatment persistence was also evaluated.

Results

In total, 195 biologic-naïve patients (109 UC; 86 CD) were included. In UC, VDZ was superior to ADM regarding endoscopic remission (29% vs 11%, P = .03), endoscopic improvement (51% vs 26%, P = .01) at week 52, and treatment persistence (P = .04). In CD, no differences in endoscopic remission (VDZ 48% vs ADM 60%; P=0.37), improvement (VDZ 76% vs ADM 77%; P = 1.00), or treatment persistence (P=0.43) at weeks 26-52 were seen. Safety profiles were similar in UC and CD.

Conclusions

This real-world cohort study of biologic-naïve IBD patients found VDZ to be superior to ADM as first-line treatment for patients with UC—but not CD—regarding endoscopic remission at week 52 and treatment persistence.
Open thoracoabdominal aortic aneurysm repair in a patient with myasthenia gravis
Authors: Uehara Kyokun , Fujiwara Yasue , Morishima Manabu , Iwakura Atsushi
Published: November 2021

Myasthenia gravis (MG) is an autoimmune neuromuscular junction disorder and rarely coexists with aortic aneurysms requiring open repair. A 66-year-old patient with MG underwent extended thoraco-abdominal aortic aneurysm (TAAA) repair 16 years after onset of type-B acute aortic dissection. At 62 years, the patient was diagnosed with MG (MGFA class IIIa) from positive anti-acetylcholine receptor antibody without thymoma. Preoperatively, MG was well-controlled by prednisolone, cyclosporin and pyridostigmine. Extent II TAAA repair was performed under general anaesthesia maintained by total intravenous anaesthesia. Transcranial motor-evoked potential and somatosensory-evoked potential were applied to monitor intraoperative spinal cord ischaemia and muscle weakness. Amplitudes of motor-evoked potential and somatosensory-evoked potential attenuated intraoperatively but normalized after reperfusion from the reconstructed tube graft. Perioperative steroid coverage was given against surgical stress. The patient was weaned from mechanical ventilatory support on postoperative day 7. No signs of spinal cord ischaemia or muscle weakness were seen.
Group BStreptococcusCapsular Serotype Alters Vaginal Colonization Fitness
Authors: Dammann Allison N , Chamby Anna B , Gonzalez Francisco J , Sharp Molly E , Flores Karina , Shahi Ifrah , Dongas Sophia , Hooven Thomas A , Ratner Adam J
Published: November 2021

Background

Group B Streptococcus (GBS) remains a leading cause of infant morbidity and mortality. A candidate vaccine targets 6 GBS serotypes, offering a potential alternative to intrapartum antibiotic prophylaxis to reduce disease burden. However, our understanding of the contributions of specific capsule types to GBS colonization and disease remains limited.

Methods

Using allelic exchange, we generated isogenic GBS strains differing only in the serotype-determining region in 2 genetic backgrounds, including the hypervirulent clonal complex (CC) 17. Using a murine model of vaginal cocolonization, we evaluated the roles of the presence of capsule and of expression of specific capsular types in GBS vaginal colonization fitness independent of other genetic factors.

Results

Encapsulated wild-type strains COH1 (CC17, serotype III) and A909 (non-CC17, serotype Ia) outcompeted isogenic acapsular mutants in murine vaginal cocolonization. COH1 wild type outcompeted A909. Notably, expression of type Ia capsule conferred an advantage over type III capsule in both genetic backgrounds.

Conclusions

Specific capsule types may provide an advantage in GBS vaginal colonization in vivo. However, success of certain GBS lineages, including CC17, likely involves both capsule and noncapsule genetic elements. Capsule switching in GBS, a potential outcome of conjugate vaccine programs, may alter colonization fitness or pathogenesis.
Estimation of SARS-CoV-2 Infection Fatality Rate by Age and Comorbidity Status Using Antibody Screening of Blood Donors During the COVID-19 Epidemic in Denmark
Authors: Kaspersen Kathrine Agergård , Hindhede Lotte , Boldsen Jens Kjærgaard , Mikkelsen Susan , Vestergaard Lasse Skafte , Berthelsen Ann-Sofie Nicole , Moustsen-Helms Ida Rask , Holm Dorte Kinggaard , Nilsson Anna Christine , Sækmose Susanne Gjørup , Sørensen Erik , Harritshøj Lene Holm , Aagaard Bitten , Hjalgrim Henrik , Lillevang Søren Thue , Jørgensen Charlotte Sværke , Krause Tyra Grove , Ullum Henrik , Pedersen Ole Birger Vestager , Ostrowski Sisse Rye , Erikstrup Christian
Published: November 2021

Background

Studies presenting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection fatality rate (IFR) for healthy individuals are warranted. We estimate IFR by age and comorbidity status using data from a large serosurvey among Danish blood donors and nationwide data on coronavirus disease 2019 (COVID-19) mortality.

Methods

Danish blood donors aged 17–69 years donating blood October 2020–February 2021 were tested with a commercial SARS-CoV-2 total antibody assay. IFR was estimated for weeks 11 to 42, 2020 and week 43, 2020 to week 6, 2021, representing the first 2 waves of COVID-19 epidemic in Denmark.

Results

In total, 84944 blood donors were tested for antibodies. The seroprevalence was 2% in October 2020 and 7% in February 2021. Among 3898039 Danish residents aged 17–69 years, 249 deaths were recorded. The IFR was low for people <51 years without comorbidity during the 2 waves (combined IFR=3.36 per 100000 infections). The IFR was below 3‰ for people aged 61–69 years without comorbidity. IFR increased with age and comorbidity but declined from the first to second wave.

Conclusions

In this nationwide study, the IFR was very low among people <51 years without comorbidity.
Interruption of initial patient assessment in the emergency department and its effect on patient perception of care quality
Authors: Johnson Kimberly D , Lindsell Christopher J , Froehle Craig , Gillespie Gordon Lee
Published: November 2021

Background

Triage is a critical first step in appropriately caring for patients in the emergency department (ED). Patients’ assumptions of the care they will receive can be established in triage. Interruptions to this process can disrupt patient flow, cause errors and lead to patient dissatisfaction.

Objective

The purpose of this study was to determine how the frequency and duration of interruptions during triage are associated with errors, patient satisfaction and patient’s perception of the care they received.

Methods

Prospective, observational, cohort study conducted in the ED of a Level 1 trauma center. Interruptions were measured using time-and-motion observations of triage interviews performed by nurses and physicians. Patients were surveyed immediately after triage interviews were complete.

Results

Surveys were completed for 178 observations. In total, 62.9% of the observations were interrupted between 1 and 5 times. While interruptions did not significantly influence patient satisfaction directly, interruptions positively influenced triage duration, which was negatively associated with patient satisfaction. Increased errors were associated with increasing frequency of triage interruptions. Triage interruptions were not associated with either patient satisfaction or perceived caregiver competence. Overall, the majority (76.6%) of patients were satisfied with their care; patient satisfaction was associated with the perceived competency of caregivers but was not associated with errors.

Conclusion

Interruptions are associated with increased errors and delays in patient care. Although increased triage duration adversely affected patient satisfaction, patients’ perceptions were not influenced by interruptions. While patient satisfaction is essential, a lack of association between patient satisfaction and errors suggests that using patient satisfaction as a measure of care quality may omit important safety information.
BSAC Vanguard Series: Antimicrobial resistance and the future of diagnostic testing
Authors: Hayward Gail , Turner Philip
Published: November 2021

In the UK around 80% of all antibiotics are prescribed by GP surgeries and in other community settings. Diagnostic technology has the potential to enable transformative change to antimicrobial stewardship in these settings. Current diagnostics are limited in terms of the information they can provide prescribers and the speed they can provide it. We need to develop diagnostics that are cheap enough for mass application, rapid enough to change prescribing decisions and able to detect bacteria which are causing disease rather than simply resident in the host. To be successful in creating change these diagnostics need to be appropriately funded and embedded in practice in ways which encourage change in prescribing behaviours. Collaboration between academia and industry is key in developing tests which are fit for purpose, well evidenced and appropriately funded in these settings.
Advances in the Use of Electrospun Nanofibrous Polymeric Matrix for Dermal Healing at the Donor Site After the Split-Thickness Skin Graft Excision: A Prospective, Randomized, Controlled, Open-Label, Multicenter Study
Authors: Haik Josef , Ullman Yehuda , Gur Eyal , Ad-El Dean , Egozi Dana , Kruchevsky Dani , Zissman Sivan , Biros Erik , Nir Rony-Reuven , Kornhaber Rachel , Cleary Michelle , Harats Moti
Published: November 2021

Dressings used to manage donor site wounds (DSWs) have up to 40% of patients experiencing complications that may cause suboptimal scarring. We evaluated the efficacy and safety of a portable electrospun nanofibrous matrix that provides contactless management of DSWs compared with standard dressing techniques. This study included adult patients who underwent an excised split-thickness skin graft (STSG) with a DSW area of 10 to 200 cm2. Patients were allocated into two groups; ie, the nanofiber group managed with a nanofibrous polymer-based matrix, and the control group managed using the standard of care such as Jelonet® or Biatain® Ibu dressing. Primary outcomes were postoperative dermal healing efficacy assessed by Draize scores. The time to complete re-epithelialization was also recorded. Secondary outcomes included postoperative adverse events, pain, and infections during the first 21 days and extended 12-month follow-up. The itching and scarring were recorded during the extended follow-up (months 1, 3, 6, 9, and 12) using Numerical-Analogue-Score and Vancouver scores, respectively. The nanofiber and control groups included 21 and 20 patients, respectively. The Draize dermal irritation scores were significantly lower in the nanofiber vs control group (Z = −2.509; P = .028) on the first postoperative day but became similar afterward (Z ≥ −1.62; P ≥ .198). In addition, the average time to re-epithelialization was similar in the nanofiber (17.9 ± 4.4 days) and control group (18.3 ± 4.5 days; Z = −0.299; P = .764), so were postoperative adverse events, pain, and infection incidence, itching and scarring. The safety and efficacy of electrospun nanofibrous matrix are similar to standard wound care allowing its use as an alternative donor site dressing following the STSG excision.
Eruptions of the Sacred in a South Indian Fishing Village
Authors: Nuckolls Charles W
Published: November 2021

Eliade invented the term ‘hierophany’ to refer to a physical manifestation of the sacred. For Eliade, all hierophanies are prefigurations of the one great manifestation, the incarnation of Christ. One can dispense with this limiting feature, and instead make use of Eliade’s more tractable theory—namely, that hierophanies are composite structures, made up of elements in contradiction with each other. Here, the hierophany to be examined is a ‘snake mound’ that suddenly appeared in a residential structure in Jalaripet, a fishing caste village in southeastern India. This article argues that the mound is a response to mutually exclusive kinship ideals, one focused on patrilineal solidarity in the form of fraternal unity and the other focuses on fraternal separation and the breakdown of patrilineal solidarity. The mound represents both ideals at a particular moment in the course of family development when the opposition between the two is most intense.
‘Hit and Miss’? Access to Legal Assistance in Immigration Detention
Authors: Lindley Anna
Published: November 2021

In the context of significant cuts to legal aid in the last decade in the UK, immigration detention remains in scope: indeed the argument that detention is legitimate rests partly on the claim that people can challenge it. Drawing on interviews with legal professionals combined with published data and reports, this article concludes that while the publicly funded Detention Duty Advice Scheme delivers advice and representation to many people held in detention, variations in access and quality give grounds for concern. Many people are also forced to rely on private solicitors, pro-bono provision and their own legal capabilities, raising questions regarding effective remedy and equality of access to justice. The article reviews the limited information available on representation types and rates, outcomes, and the wider impact of access to legal representation or the lack of it. The conclusion summarizes key points and explores ways forward.
Mink (Neovison vison) fecal microbiomes are influenced by sex, temperature, and time postdefecation
Authors: Lafferty Diana J R , Gillman Sierra J , Jeakle Lane K , Roell Brian J , McKenney Erin A
Published: November 2021

Gut microbiomes encode myriad metabolic functions critical to mammalian ecology and evolution. While fresh fecal samples provide an efficient, noninvasive method of sampling gut microbiomes, collecting fresh feces from elusive species is logistically challenging. Nonfresh feces, however, may not accurately represent the gut microbiome of the host due to succession of gut microbial consortia postdefecation as well as colonization by microbes from the surrounding environment. Using American mink (Neovison vison) as a model species, we examined postdefecation microbial community succession to learn how ambient temperature and temporal sampling constraints influence the reliability of nonfresh feces to represent host gut microbiomes. To achieve our goal, we analyzed fresh mink feces (n = 5 females; n = 5 males) collected at the time of defecation from captive mink at a farm in the Upper Peninsula of Michigan and we subsequently subsampled each fecal specimen to investigate microbial community succession over five days, under both warm (21°C) and cold (–17°C to –1°C) temperature treatments. We found that both temperature and time influenced fecal microbiome composition; and we also detected significant sexual dimorphism in microbial community structures, with female mink microbiomes exhibiting significantly greater variation than males’ when exposed to the warm temperature treatment. Our results demonstrate that feces from unknown individuals can be a powerful tool for examining carnivore gut microbiomes, though rigorous study design is required because sex, ambient temperature, and time since defecation drive significant microbial variation and the sample size requirements necessary for detecting statistically significant differences between target populations is an important consideration for future ecologically meaningful research.
Nucleolar GTPase Bms1 displaces Ttf1 from RFB-sites to balance progression of rDNA transcription and replication
Authors: Zhu Yanqing , Wang Yong , Tao Boxiang , Han Jinhua , Chen Hong , Zhu Qinfang , Huang Ling , He Yinan , Hong Jian , Li Yunqin , Chen Jun , Huang Jun , Lo Li Jan , Peng Jinrong
Published: November 2021

18S, 5.8S, and 28S ribosomal RNAs (rRNAs) are cotranscribed as a pre-ribosomal RNA (pre-rRNA) from the rDNA by RNA polymerase I whose activity is vigorous during the S-phase, leading to a conflict with rDNA replication. This conflict is resolved partly by replication-fork-barrier (RFB)-sites sequences located downstream of the rDNA and RFB-binding proteins such as Ttf1. However, how Ttf1 is displaced from RFB-sites to allow replication fork progression remains elusive. Here, we reported that loss-of-function of Bms1l, a nucleolar GTPase, upregulates rDNA transcription, causes replication-fork stall, and arrests cell cycle at the S-to-G2 transition; however, the G1-to-S transition is constitutively active characterized by persisting DNA synthesis. Concomitantly, ubf, tif-IA, and taf1b marking rDNA transcription, Chk2, Rad51, and p53 marking DNA-damage response, and Rpa2, PCNA, Fen1, and Ttf1 marking replication fork stall are all highly elevated in bms1l mutants. We found that Bms1 interacts with Ttf1 in addition to Rc1l. Finally, we identified RFB-sites for zebrafish Ttf1 through chromatin immunoprecipitation sequencing and showed that Bms1 disassociates the Ttf1‒RFB complex with its GTPase activity. We propose that Bms1 functions to balance rDNA transcription and replication at the S-phase through interaction with Rcl1 and Ttf1, respectively. TTF1 and Bms1 together might impose an S-phase checkpoint at the rDNA loci.
Read-once refutations in Horn constraint systems: an algorithmic approach
Authors: Subramani K , Wojciechowski Piotr , Sheng Ying
Published: November 2021

In this paper, we discuss exact and parameterized algorithms for the problem of finding a read-once refutation (ROR) in an unsatisfiable Horn constraint system (HCS). Recall that a linear constraint system $\mathbf {A \cdot x \ge b}$ is said to be an HCS if each entry in $\textbf {A}$ belongs to the set $\{0,1,-1\}$ and at most one entry in each row of $\textbf {A}$ is positive. In this paper, we examine the importance of constraints in which more variables have negative coefficients than positive coefficients. In particular, we study the impact of the proportion of these ‘net-negative’ constraints has on the difficulty of finding RORs. There exist several algorithms for checking whether an HCS is feasible. To the best of our knowledge, these algorithms are not certifying, i.e. they do not provide a certificate of infeasibility. Our work is concerned with providing a specialized class of certificates called ‘read-once refutations’. In an ROR, each constraint defining the HCS may be used at most once in the derivation of a refutation. The problem of checking if an HCS has an ROR has been shown to be NP-hard. We analyse the HCS ROR problem from three different algorithmic perspectives, viz., parameterized algorithms, exact exponential algorithms and approximation algorithms. In particular, we show that the HCS ROR problem is fixed-parameter tractable (FPT) with respect to the number of constraints in the system that have more variables with negative coefficient than variables with positive coefficient. Additionally, we show that the HCS ROR problem becomes easy when this parameter is both small and large. We also derive an algorithm that runs in time $O(1.66^{m})$, where $m$ is the number of constraints in the HCS. On the lower-bound side, we derive a lower bound on the algorithmic resources needed for this problem using the Exponential Time Hypothesis. We also establish that the HCS ROR problem does not have a polynomial kernel when the number of constraints with three or more variables in a refutation is used as a parameter. Finally, we show that the problem of approximating the length of the shortest ROR in an HCS is NPO PB-complete1.
7Be in the outburst of the ONe nova V6595 Sgr
Authors: Molaro P , Izzo L , D’Odorico V , Aydi E , Bonifacio P , Cescutti G , Harvey E J , Hernanz M , Selvelli P , della Valle M
Published: November 2021

We report on the search for the 7Be ii isotope in the outbursts of the classical nova V6595 Sgr by means of high-resolution Ultraviolet and Visual Echelle Spectrograph (UVES) observations taken at the European Southern Observatory’s Very Large Telescope in 2021 April, about two weeks after its discovery and under difficult circumstances due to the pandemic. Narrow absorption components with velocities at ∼ −2620 and −2820 km s−1, superposed on broader and shallow absorption, are observed in the outburst spectra for the 7Be ii λλ313.0583, 313.1228 nm doublet resonance lines, as well as in several other elements such as Ca ii, Fe i, Mg i, Na i, H i and Li i. Using the Ca ii K line as a reference element, we infer N(7Be)/N(H) ≈ 7.4 × 10−6, or ≈ 9.8 × 10−6 when the 7Be decay is taken into account. The 7Be abundance is about half of the value most frequently measured in novae. The possible presence of overionization in the layers where 7Be ii is detected is also discussed. Observations taken at the Telescopio Nazionale Galileo in La Palma 91 days after discovery showed prominent emission lines of oxygen and neon, which allow us to classify the nova as ONe type. Therefore, although 7Be is expected to be higher in CO novae, it is found at comparable levels in both nova types.
PHANGS-HST: new methods for star cluster identification in nearby galaxies
Authors: Thilker David A , Whitmore Bradley C , Lee Janice C , Deger Sinan , Chandar Rupali , Larson Kirsten L , Hannon Stephen , Ubeda Leonardo , Dale Daniel A , Glover Simon C O , Grasha Kathryn , Klessen Ralf S , Kruijssen J M Diederik , Rosolowsky Erik , Schruba Andreas , White Richard L , Williams Thomas G
Published: November 2021

We present an innovative and widely applicable approach for the detection and classification of stellar clusters, developed for the PHANGS-HST Treasury Program, an NUV-to-I band imaging campaign of 38 spiral galaxies. Our pipeline first generates a unified master source list for stars and candidate clusters, to enable a self-consistent inventory of all star formation products. To distinguish cluster candidates from stars, we introduce the Multiple Concentration Index (MCI) parameter, and measure inner and outer MCIs to probe morphology in more detail than with a single, standard concentration index (CI). We improve upon cluster candidate selection, jointly basing our criteria on expectations for MCI derived from synthetic cluster populations and existing cluster catalogues, yielding model and semi-empirical selection regions (respectively). Selection purity (confirmed clusters versus candidates, assessed via human-based classification) is high (up to 70 per cent) for moderately luminous sources in the semi-empirical selection region, and somewhat lower overall (outside the region or fainter). The number of candidates rises steeply with decreasing luminosity, but pipeline-integrated Machine Learning (ML) classification prevents this from being problematic. We quantify the performance of our PHANGS-HST methods in comparison to LEGUS for a sample of four galaxies in common to both surveys, finding overall agreement with 50–75 per cent of human verified star clusters appearing in both catalogues, but also subtle differences attributable to specific choices adopted by each project. The PHANGS-HST ML-classified Class 1 or 2 catalogues reach ∼1 mag fainter, ∼2 × lower stellar mass, and are 2−5 × larger in number, than attained in the human classified samples.
The fate of icy pebbles undergoing sublimation in protoplanetary discs
Authors: Spadaccia Stefano , Capelo Holly L , Pommerol Antoine , Schuetz Philipp , Alibert Yann , Ros Katrin , Thomas Nicolas
Published: November 2021

Icy pebbles may play an important role in planet formation close to the water ice line of protoplanetary discs. There, dust coagulation is more efficient and re-condensation of vapor on pebbles may enhance their growth outside the ice line. Previous theoretical studies showed that disruption of icy pebbles due to sublimation increases the growth rate of pebbles inside and outside the ice line, by freeing small silicate particles back in the dust reservoir of the disc. However, since planet accretion is dependent on the Stokes number of the accreting pebbles, the growth of planetesimals could be enhanced downstream of the ice line if pebbles are not disrupting upon sublimation. We developed two experimental models of icy pebbles using different silicate dusts, and we exposed them to low-temperature and low-pressure conditions in a vacuum chamber. Increasing the temperature inside the chamber, we studied the conditions for which pebbles are preserved through sublimation without disrupting. We find that small silicate particles (<50 μm) and a small quantity of ice (around 15 per cent pebble mass) are optimal conditions for preserving pebbles through sublimation. Furthermore, pebbles with coarse dust distribution (100–300 μm) do not disrupt if a small percentage (10–20 per cent mass) of dust grains are smaller than 50 μm. Our findings highlight how sublimation is not necessarily causing disruption, and that pebbles seem to survive fast sublimation processes effectively.
Gamma-rays from reaccelerated cosmic rays in high-velocity clouds colliding with the Galactic disc
Authors: del Valle Maria V
Published: November 2021

High-velocity clouds moving towards the disc will reach the Galactic plane and will inevitably collide with the disc. In these collisions, a system of two shocks is produced, one propagating through the disc and the other develops within the cloud. The shocks produced within the clouds in these interactions have velocities of hundreds of kilometres per second. When these shocks are radiative they may be inefficient in accelerating fresh particles; however, they can reaccelerate and compress Galactic cosmic rays from the background. In this work, we investigate the interactions of Galactic cosmic rays within a shocked high-velocity cloud, when the shock is induced by the collision with the disc. This study is focused in the case of radiative shocks. We aim to establish under which conditions these interactions lead to significant non-thermal emission, especially gamma-rays. We model the interaction of cosmic ray protons and electrons reaccelerated and further energized by compression in shocks within the clouds, under very general assumptions. We also consider secondary electron–positron pairs produced by the cosmic ray protons when colliding with the material of the cloud. We conclude that nearby clouds reaccelerating Galactic cosmic rays in local shocks can produce high-energy radiation that might be detectable with existing and future gamma-ray detectors. The emission produced by electrons and secondary pairs is important at radio wavelengths, and in some cases it may be relevant at hard X-rays. Concerning higher energies, the leptonic contribution to the spectral energy distribution is significant at soft gamma-rays.
Possibility of transporting material from Ceres to NEO region via 8:3 MMR with Jupiter
Authors: Kováčová M , Kornoš L , Matlovič P
Published: November 2021

In this work, we investigate the possibility of transporting material to the NEO region via the 8:3 MMR with Jupiter, potentially even material released from the dwarf planet Ceres. By applying the FLI map method to the 8:3 MMR region in the orbital plane of Ceres, we were able to distinguish between stable and unstable orbits. Subsequently, based on the FLI maps (for mean anomaly M = 60° and also M = 30°), 500 of the most stable and 500 of the most unstable particles were integrated for $15\, \mathrm{Myr}$ for each map. Long-term integration in the case of M = 60° showed that most of the stable particles evolved, in general, in uneventful ways with only 0.8 per cent of particles reaching the limit of q ≤ 1.3 au . However, in the case of M = 30°, a stable evolution was not confirmed. Over 40 per cent of particles reached orbits with q ≤ 1.3 au and numerous particles were ejected to hyperbolic orbits or orbits with a > 100 au. The results for stable particles indicate that short-term FLI maps are more suitable for finding chaotic orbits, than for detecting the stable ones. A rough estimate shows that it is possible for material released from Ceres to get to the region of 8:3 MMR with Jupiter. A long-term integration of unstable particles in both cases showed that transportation of material via 8:3 MMR close to the Earth is possible.
Forming short-period substellar companions in 47 Tucanae – I. Dynamical model and brown dwarf tidal capture rates
Authors: Winter Andrew J , Rosotti Giovanni P , Clarke Cathie , Giersz Mirek
Published: November 2021

Stars in globular clusters formed and evolved in the most extreme environment: high density and low metallicity. If the formation of stars and planets is at all sensitive to environmental conditions, this should therefore be evident in globular clusters. Observations have indicated that hot Jupiters are at least an order of magnitude less prevalent in the central region of the globular cluster 47 Tucanae (Tuc) than in the field. In this work, we explore the claims in the literature for additional consequences for the low-mass stellar initial mass function. Tidal capture, the mechanism that produces X-ray binaries in globular clusters, applies also to brown dwarfs (BDs). This process produces tight stellar–BD binaries that would be detectable by transit surveys. Applying a Monte Carlo dynamical evolution model, we compute the overall BD capture rates. We find that the number of captures is lower than previous estimates. Capture efficiency increases steeply with stellar mass, which means that mass segregation reduces capture efficiency as BDs and low-mass stars occupy the same regions. The result of this effect is that the current constraints on the short-period companion fraction remain marginally consistent with initially equal numbers of BDs and stars. However, our findings suggest that expanding the sample in 47 Tuc or surveying other globular clusters for close substellar companions can yield constraints on the substellar initial mass function in these environments. We estimate the capture rates in other globular clusters and suggest that 47 Tuc remains a promising target for future transit surveys.
ESO 137-002: a large spiral undergoing edge-on ram-pressure stripping with little star formation in the tail
Authors: Laudari Sunil , Jáchym Pavel , Sun Ming , Waldron Will , Chatzikos Marios , Kenney Jeffrey , Luo Rongxin , Nulsen Paul , Sarazin Craig , Combes Françoise , Edge Tim , Voit Mark , Donahue Megan , Cortese Luca
Published: November 2021

Ram pressure stripping (RPS) is an important mechanism for galaxy evolution. In this work, we present results from HST and APEX observations of one RPS galaxy, ESO 137-002 in the closest rich cluster Abell 3627. The galaxy is known to host prominent X-ray and H α tails. The HST data reveal significant features indicative of RPS in the galaxy, including asymmetric distribution of dust in the galaxy, dust filaments, and dust clouds in ablation generally aligned with the direction of ram pressure, and young star clusters immediately upstream of the residual dust clouds that suggest star formation (SF) triggered by RPS. The distribution of the molecular gas is asymmetric in the galaxy, with no CO upstream and abundant CO downstream and in the inner tail region. A total amount of ∼5.5 × 109 M⊙ of molecular gas is detected in the galaxy and its tail. On the other hand, we do not detect any active SF in the X-ray and H α tails of ESO 137-002 with the HST data and place a limit on the SF efficiency in the tail. Hence, if selected by SF behind the galaxy in the optical or UV (e.g. surveys like GASP or using the Galex data), ESO 137-002 will not be considered a ‘jellyfish’ galaxy. Thus, galaxies like ESO 137-002 are important for our comprehensive understanding of RPS galaxies and the evolution of the stripped material. ESO 137-002 also presents a great example of an edge-on galaxy experiencing a nearly edge-on RPS wind.
The nuclear and cytoplasmic activities of RNA polymerase III, and an evolving transcriptome for surveillance
Authors: Kessler Alan C , Maraia Richard J
Published: November 2021

A 1969 report that described biochemical and activity properties of the three eukaryotic RNA polymerases revealed Pol III as highly distinguishable, even before its transcripts were identified. Now known to be the most complex, Pol III contains several stably-associated subunits referred to as built-in transcription factors (BITFs) that enable highly efficient RNA synthesis by a unique termination-associated recycling process. In vertebrates, subunit RPC7(α/β) can be of two forms, encoded by POLR3G or POLR3GL, with differential activity. Here we review promoter-dependent transcription by Pol III as an evolutionary perspective of eukaryotic tRNA expression. Pol III also provides nonconventional functions reportedly by promoter-independent transcription, one of which is RNA synthesis from DNA 3′-ends during repair. Another is synthesis of 5′ppp-RNA signaling molecules from cytoplasmic viral DNA in a pathway of interferon activation that is dysfunctional in immunocompromised patients with mutations in Pol III subunits. These unconventional functions are also reviewed, including evidence that link them to the BITF subunits. We also review data on a fraction of the human Pol III transcriptome that evolved to include vault RNAs and snaRs with activities related to differentiation, and in innate immune and tumor surveillance. The Pol III of higher eukaryotes does considerably more than housekeeping.