bluuebunny/crossref_metadata_2025 huggingface dataset:
HGF Spatial–Spectral Fusion Method for Hyperspectral Images
Authors: Fu Pingjie , Zhang Yuxuan , Meng Fei , Zhang Wei , Zhang Banghua
Published: December 2022

Quantitative studies on surface elements require satellite hyperspectral images with high spatial resolution. The identification of different surface elements requires different characteristic bands and their corresponding optimal spatial–spectral fusion methods. To address these problems, the harmonic analysis (HA), guided filtering, and Gram–Schmidt (GS) algorithms were integrated to propose a spatial–spectral fusion method called HGF. The fusion experiment and validation of the hyperspectral images of GaoFen-5 (GF-5) and ZY1-02D were conducted separately using the HGF method, and the fusion effect was evaluated in three band intervals according to the spectral response of the ground class. First, HGF was used to fuse the GF-5 and GaoFen-1 (GF-1) images, and the fusion effect was evaluated both qualitatively and quantitatively. Second, the optimal fusion method was selected for the corresponding characteristic bands of the different surface elements. Finally, the hyperspectral image obtained by ZY1-02D and multispectral image of Sentinel-2B were used for validation to improve the accuracy and efficiency of satellite hyperspectral images in quantitative studies. The results show that for further studies on soil, vegetation, and water bodies, the best fusion methods in the 390–730, 730–1400, and 1400–2260 nm intervals are the GS, HGF, and HGF algorithms, respectively. Further analysis showed that the HGF or GS methods can be selected for quantitative studies on vegetation and water bodies and that the HGF method exhibits outstanding advantages for quantitative analysis of each soil element.
A Systematical Comparison of Catalytic Behavior of NM/γ-Al2O3 (NM = Ru, Rh, Pt, Pd, Au, Ir) on 1,2-Dichloroethane Oxidation: Distributions of By-Products and Reaction Mechanism
Authors: Li Lu , Zhang Jingjie , Xu Han , Tian Mingjiao , He Chi
Published: December 2022

Understanding the reaction path and mechanism of chlorinated volatile organic compound (CVOC) destruction is important for designing efficient catalysts, especially for the application of noble metal-based materials. Herein, several typical noble metals, Ru, Rh, Pt, Pd, Au, and Ir, supported on γ-Al2O3 catalysts were synthesized by the hydrazine hydrate reduction method for 1,2-dichloroethane (1,2-DCE) elimination. Various character measurements were conducted, and the results suggest that the high-valence state of noble metals is beneficial for the 1,2-DCE reaction as it enables the enhancement of the mobility of the surficial active oxygen species of catalysts. Among the noble metals, Ru/γ-Al2O3 expresses superior catalytic reactivity, with a 90% pollutant conversion rate at 337 °C, and competitive CO2 selectivity, 99.15% at the temperature of total oxidation. The distribution of by-products and the degradation routes were analyzed online by GC-ECD and in situ diffuse reflectance infrared spectroscopy, which may provide helpful insight for the future application of noble metal-based catalysts for CVOC elimination in industrial fields.
Research on Yaw Stability Control Method of Liquid Tank Semi-Trailer on Low-Adhesion Road under Turning Condition
Authors: Li Gangyan , Fu Teng , Zhao Ran
Published: December 2022

To ensure that a liquid tank semi-trailer has good yaw stability and path-following performance on low-adhesion roads under turning conditions, a multi-object PID differential braking-control method is proposed, which takes the tractor yaw rate, semi-trailer yaw rate, and articulation angle as the control parameters. According to the principle of equivalence, the trammel pendulum (TP) model is used to simulate the liquid sloshing effect in the liquid tank, and the Fluent software is used to identify the key parameters of the trammel pendulum model and verify the correctness of its simulation effect. Then, a co-simulation model is built based on TruckSim and MATLAB/Simulink. Based on the simplified six degrees of freedom model and the co-simulation model of a liquid tank semi-trailer, a multi-object PID differential braking-control method is designed, and the vehicle state responses with and without control are compared when it is turning on a low-adhesion road. The simulation results show that the proposed multi-object PID differential braking-control method can effectively improve the yaw stability and path-following performance of the liquid tank semi-trailer when turning on a low-adhesion road.
Prevalence and Outcome of Asthma in Adult Patients Admitted to the Emergency Department for COVID-19: A Case-Control Study
Authors: Gabrielli Maurizio , Candelli Marcello , Pignataro Giulia , Ojetti Veronica , Sacco Fernandez Marta , Rosa Federico , Gasbarrini Antonio , Franceschi Francesco ,   on behalf of GEMELLI AGAINST COVID 2019
Published: December 2022

(1) Background: Viral respiratory infections are common triggers for asthma exacerbation, often leading patients to the emergency department (ED). COVID-19, the disease caused by the SARS-CoV-2 virus, typically presents with respiratory symptoms, from minor symptoms, up to and including severe acute respiratory failure. Data on the association between asthma and COVID-19 are conflicting, and those from an ED setting are scarce. Our aims were to assess the prevalence and outcome of patients with asthma admitted to the ED for COVID-19. (2) Methods: We performed a case-control study, extracting data from a registry of adult patients with confirmed COVID-19 consecutively admitted to the ED of our hospital between March 2020 and January 2021. (3) Results: We identified 83 patients with asthma out of 935 individuals (prevalence 8.9%). There were no significant differences between cases and controls regarding both the proportion of hospital admissions and patients with critical COVID-19. (OR 1.37; 95% CI 0.52–3.56; and (OR 0.74; 95% CI 0.31–1.78 respectively). (4) Conclusions: In patients admitted to the ED for COVID-19, the prevalence of asthma was not higher than expected, and asthma was not associated with a worse outcome, in terms of the rate of hospitalization and critical COVID-19 disease.
A New Approach to Identifying an Arbitrary Number of Inclusions, Their Geometry and Location in the Structure Using Topological Optimization
Authors: Krysko A. V. , Makseev Anton , Smirnov Anton , Zhigalov M. V. , Krysko V. A.
Published: December 2022

In the present paper, a new approach to identifying an arbitrary number of inclusions, their geometry and their location in 2D and 3D structures using topological optimization was proposed. The new approach was based on the lack of initial information about the geometry of the inclusions and their location in the structure. The numerical solutions were obtained by the finite element method in combination with the method of moving asymptotes. The convergence of the finite element method at the coincidence of functions and their derivatives was analyzed. Results with an error of no more than 0.5%, i.e., almost exact solutions, were obtained. Identification at impact on the plate temperature and heat flux by solving the inverse problem of heat conduction was produced. Topological optimization for identifying an arbitrary number of inclusions, their geometry and their location in 2D problems was investigated.
Investigation of Mechanical Properties of Al/CNT Nanocomposites Produced by Powder Metallurgy
Authors: Carneiro Íris , Simões Sónia
Published: December 2022

Demanding requirements in automotive and aerospace applications promote the growing need to obtain materials and advanced technology capable of combining low weight with high mechanical properties. Aluminum matrix nanocomposites could be great candidates to respond to such needs. In this sense, this investigation aims to study the mechanical properties of nanocomposites of aluminum matrices reinforced with carbon nanotubes (CNTs). The nanocomposites were produced by powder metallurgy with 1.00 vol.% of reinforcement and ultrasonication as the dispersion method. Tensile, Vickers microhardness and nanoindentation tests were carried out in different sections. Microstructural characterizations were conducted in scanning electron microscopy (SEM) and electron backscattered diffraction (EBSD) to understand and relate to the mechanical properties. An increase in the yield strength of 185% was observed for the nanocomposites, which can be attributed to the load transfer mechanism. However, the CNTs observed at the grain boundaries promote a decrease in the ductility of the nanocomposites. The mechanical behavior of the nanocomposites was further investigated by EBSD observation. The results revealed that the nanocomposites have a less extensive area of plastic deformation than the Al matrix, which is consistent with the tensile results. The presence of reinforcement affects the lattice rotation during the tensile test and the active slip systems, thus affecting their deformation behavior.
Production and Characterization of a β-Cyclodextrin Inclusion Complex with Platonia insignis Seed Extract as a Proposal for a Gastroprotective System
Authors: Lima Nascimento Juliana , Coelho Angélica Gomes , Oliveira Barros Ytallo Samuel , Sousa Oliveira Irisdalva , Vieira da Silva Francilene , Custódio Viana Ana Flávia Seraine , Araújo Bruno Quirino , dos Santos Rocha Márcio , das Chagas Pereira de Andrade Francisco , de Oliveira Barbosa Celma , de Barros Fernandes Hélio , Mendes Anderson Nogueira , da Costa-Júnior Joaquim Soares , Oliveira Rita de Cássia Meneses , Lucarini Massimo , Durazzo Alessandra , Arcanjo Daniel Dias Rufino , Citó Antônia Maria das Graças Lopes
Published: December 2022

Platonia insignis Mart, Clusiaceae, known as bacuri, is a species native to Brazil that, in studies with extract of the seed of its fruit, showed antioxidant activity against free radicals. Products with such properties may be of great importance in the treatment of peptic ulcers since this pathology may be associated with the inflammatory process caused by the action of free radicals. Cyclodextrins are molecules capable of forming inclusion complexes with other molecules, affecting their physicochemical properties and improving their pharmacokinetic characteristics. Thus, this work aimed to produce, characterize, and evaluate the gastroprotective effect of the inclusion complex of β-cyclodextrin (β-CD) with the bacuri seeds hexanic extract (BSHE). In the characterization of the inclusion complex, an apparent stability constant (Kc) of 416 mol/L was obtained in the solubility study; the BSHE:β-CD m/m (g) complexation ratios at 1:9, 2:8, and 3:7 were 5.51%, 21.46%, and 20.11%, respectively. The formation of the BSHE:β-CD inclusion complex was observed by FTIR technique, indicating the disappearance of bands characteristic of BSHE (2960 cm−1 and 1755 cm−1) when in the complex, compared to the spectra of pure BSHE or in physical mixture with β-CD, and by X-ray diffraction, which indicated a loss of crystallinity, typical signals of pure β-CD, and presentation of intense amorphization, characteristic of BSHE, incorporated in the β-CD pockets. In the evaluation of gastroprotective activity, through absolute ethanol-induced gastric lesions in mice, both BSHE and BSHE:β-CD reduced gastric lesions, with 100 mg/kg dose of the complex having the greatest gastroprotective effect. BSHE:β-CD was also able to reduce gastric lesions from ischemia and reperfusion, with the 50 mg/kg dose being the most effective. BSHE:β-CD, also at this dose, reduced the MDA levels of the gastric mucosa, indicating a possible antioxidant activity in its gastroprotective effect. Thus, it was concluded that inclusion complex formation between β-CD and BSHE is possible, and that this formulation enhanced the gastric protective activity.
Blind Image Quality Assessment with Deep Learning: A Replicability Study and Its Reproducibility in Lifelogging
Authors: Ribeiro Ricardo , Trifan Alina , Neves António J. R.
Published: December 2022

The wide availability and small size of different types of sensors have allowed for the acquisition of a huge amount of data about a person’s life in real time. With these data, usually denoted as lifelog data, we can analyze and understand personal experiences and behaviors. Most of the lifelog research has explored the use of visual data. However, a considerable amount of these images or videos are affected by different types of degradation or noise due to the non-controlled acquisition process. Image Quality Assessment can plays an essential role in lifelog research to deal with these data. We present in this paper a twofold study on the topic of blind image quality assessment. On the one hand, we explore the replication of the training process of a state-of-the-art deep learning model for blind image quality assessment in the wild. On the other hand, we present evidence that blind image quality assessment is an important pre-processing step to be further explored in the context of information retrieval in lifelogging applications. We consider that our efforts have been successful in the replication of the model training process, achieving similar results of inference when compared to the original version, while acknowledging a fair number of assumptions that we had to consider. Moreover, these assumptions motivated an extensive additional analysis that led to significant insights on the influence of both batch size and loss functions when training deep learning models in this context. We include preliminary results of the replicated model on a lifelogging dataset, as a potential reproducibility aspect to be considered.
Design Framework for Selection of Grid Topology and Rectangular Cross-Section Size of Elastic Timber Gridshells Using Genetic Optimisation
Authors: Roig Antonio , Lara-Bocanegra Antonio José , Xavier José , Majano-Majano Almudena
Published: December 2022

This work presents a design framework for the selection of the topology and cross-section size of elastic timber gridshells, taking as constraints the shape of the structure and the maximum value of bending stress that can be reached in a given area of the gridshell. For this purpose, a parametric design environment and a genetic optimisation algorithm are used, which provides a set of solutions (optimal and near-optimal) that can be examined by the designer before adopting the final solution. The construction of the parametric mesh model is based on a geometric approach using an original adaptation of the Compass Method by developing two algorithms. The first one plots geodesic curves on a surface given a starting point and a direction. The second algorithm adapts the accuracy of the Compass Method to the local curvature of the surface, substantially minimising the computation time. The results show that the optimisation process succeeds in significantly reducing the initial bending stresses and offers an interesting solution space, consisting of a set of solutions with sufficiently diverse topologies and cross-section sizes, from which the final solution can be chosen by the Decision Maker, even according to additional non-programmed structural or aesthetic requirements. The design framework has been successfully applied and verified in the design of the PEMADE gridshell, an innovative elastic timber gridshell recently realised by the authors. Finally, the most relevant details of its construction process carried out to ensure the exact position of the timber laths are presented.
Modeling the Data Provenance of Relational Databases Supporting Full-Featured SQL and Procedural Languages
Authors: Tang Deyou , Zhao Rong , Lin Yuebang , Zhang Tangqing , Zhang Pingjian
Published: December 2022

Data provenance is information about where data come from (provenance data) and how they transform (provenance transformation). Data provenance is widely used to evaluate data quality, trace errors, audit data, and understand references among data. Current studies on data provenance in relational database management systems (RDBMS) still have limitations in supporting full-featured SQL or procedural languages. With these challenges in mind, we present a formal definition of provenance data and provenance transformation for relational data. Then, we propose a solution to support data provenance in relational databases, including provenance graphs and provenance routes. Our method not only solves the complicated problem of modeling provenance in DBMS but also is capable of extending procedural languages in SQL. We also present ProvPg, a PostgreSQL-based prototype database system supporting data provenance in multiple granularities. ProvPg implements extraction, calculation, query, and visualization of provenance. We perform TPC-H tests for ProvPg and PostgreSQL, respectively. Experimental results show that ProvPg addresses the vision of supporting data provenance with little extra computation overhead for the execution engine, which indicates that our model is applicable to lineage tracing applications.
Battery Aging Models Based on High-Current Incremental Capacity in Fast Charging
Authors: Lombardi Ludovico , Ospina Agudelo Brian , Zamboni Walter , Monmasson Eric
Published: December 2022

This paper presents battery aging models based on high-current incremental capacity features in the presence of battery cycling profiles characterized by fast charging conditions. In particular, the main peak area under the incremental capacity graph is proposed as a capacity indicator. A dataset from the Toyota Research Institute is analyzed. Batteries’ cycling data are characterized by various single- or double-step fast charges in constant current to reach 80% of the battery state of charge; the remaining charge process is performed by a 1C charge. Depending on the battery, a linear or logarithmic model was identified as the best suitable for representing the capacity–peak area relationship. The generalization capabilities of the proposed models are evaluated by performing an inference analysis of the fitting results over groups of batteries. Finally, we evaluated the prediction performance of the models by adopting a cross-validation approach.
Engineering Biology of Yeast for Advanced Biomanufacturing
Authors: Jiang Wei , Li Yanjun , Peng Huadong
Published: December 2022

Advanced biomanufacturing has been widely involved in people’s daily life, such as the production of molecules used as pharmaceuticals, in foods and beverages, and in bio-fuels [...]
The Delineation and Ecological Connectivity of the Three Parallel Rivers Natural World Heritage Site
Authors: Li Hui , Guo Wanqi , Liu Yan , Zhang Qiman , Xu Qing , Wang Shuntao , Huang Xue , Xu Kexin , Wang Junzhi , Huang Yilin , Gao Wei
Published: December 2022

Landscape connectivity refers to the degree of continuity between the spatially structured units of a landscape. Ecological connectivity can characterise the degree to which ecological functional areas are connected in terms of function and ecological processes. In this study, the landscape pattern index and ecosystem service values were used to evaluate the ecological functional resistance of each landscape type, taking the Three Parallel Rivers Natural World Heritage Site as an example and the habitat distribution and population size of the Yunnan snub-nosed monkey as a reference. The minimum cost distance model, combined with the barrier impact index (BEI) and ecological connectivity index (ECI), was used to determine the degree of barrier impact on the study area and the ecological connectivity of the core reserve of the heritage site in both 2000 and 2020. The resistances of the different land types and landscape heterogeneity to the ecological function of species migration between the core protected areas of the heritage site were, in descending order, those of the forest, shrubs and grass, water, unused land, cultivated land, and built-up land. In 2020, the study area had a large BEI, with areas such as built-up areas, major roads, the sides of large rivers, and arable land being significant contributors to the blockage of landscape connectivity. The overall landscape connectivity in the study area was generally low, with clear spatial differentiation and a three-column parallel distribution pattern influenced by the topography and landscape. With the adjustment of the core reserve boundaries of the heritage site, the proportion of areas with high connectivity (ECI = 4–5) increased from 11.31% in 2000 to 34.36% in 2020. This increased landscape connectivity was conducive to the migration and reproduction of large terrestrial animals, such as the Yunnan snub-nosed monkey, with increasing numbers of populations and individuals. This study provides theoretical and methodological insights into the delineation and conservation of natural heritage sites and landscape connectivity.
Uric Acid Deteriorates Load-Free Cell Shortening of Cultured Adult Rat Ventricular Cardiomyocytes via Stimulation of Arginine Turnover
Authors: Weber Martin , Schreckenberg Rolf , Schlüter Klaus-Dieter
Published: December 2022

Hyperuricemia is a risk factor for heart disease. Cardiomyocytes produce uric acid via xanthine oxidase. The enzymatic reaction leads to oxidative stress in uric-acid-producing cells. However, extracellular uric acid is the largest scavenger of reactive oxygen species, specifically to nitrosative stress, which can directly affect cells. Here, the effect of plasma-relevant concentrations of uric acid on adult rat ventricular cardiomyocytes is analyzed. A concentration- and time-dependent reduction of load-free cell shortening is found. This is accompanied by an increased protein expression of ornithine decarboxylase, the rate-limiting enzyme of the polyamine metabolism, suggesting a higher arginine turnover. Subsequently, the effect of uric acid was attenuated if other arginine consumers, such as nitric oxide synthase, are blocked or arginine is added. In the presence of uric acid, calcium transients are increased in cardiomyocytes irrespective of the reduced cell shortening, indicating calcium desensitization. Supplementation of extracellular calcium or stimulation of intracellular calcium release by β-adrenergic receptor stimulation attenuates the uric-acid-dependent effect. The effects of uric acid are attenuated in the presence of a protein kinase C inhibitor, suggesting that the PKC-dependent phosphorylation of troponin triggers the desensitizing effect. In conclusion, high levels of uric acid stress cardiomyocytes by accelerating the arginine metabolism via the upregulation of ornithine decarboxylase.
Comprehensive Transcriptome Analysis of Different Skin Colors to Evaluate Genes Related to the Production of Pigment in Celestial Goldfish
Authors: Li Rongni , Sun Yansheng , Cui Ran , Zhang Xin
Published: December 2022

Skin color is an important phenotypic feature of vertebrate fitness under natural conditions. Celestial goldfish, a common goldfish breed in China, mainly shows three kinds of skin colors including white, yellow and brown. However, the molecular genetic basis of this phenotype is still unclear. In this study, high-throughput sequencing was carried out on the back skin tissues of celestial goldfish with different skin colors. About 58.46 Gb of original data were generated, filtered and blasted, and 74,297 mRNAs were obtained according to the reference transcriptome. A total of 4653 differentially expressed genes were screened out among the brown, yellow and white groups, and the expression of melanogenesis related genes in brown goldfish was significantly higher than the other two groups. There are 19 common differentially expressed genes among three groups, of which eight genes are related to pigment production, including tyrp1a, slc2a11b, mlana, gch2, loc113060382, loc113079820, loc113068772 and loc113059134. RT-qPCR verified that the expression patterns of randomly selected differentially expressed transcripts were highly consistent with those obtained by RNA sequencing. GO and KEGG annotation revealed that these differentially expressed genes were mostly enriched in pathways of the production of pigment, including melanogenesis, tyrosine metabolism, Wnt signaling pathway, MAPK signaling pathway etc. These results indicated that the external characteristics of goldfish are consistent with the analysis results at transcriptome level. The results of this study will lay a foundation for further study on the expression characteristics and gene network analysis of pigment related genes.
Linoleic Acid-Enriched Diet Increases Mitochondrial Tetralinoleoyl Cardiolipin, OXPHOS Protein Levels, and Uncoupling in Interscapular Brown Adipose Tissue during Diet-Induced Weight Gain
Authors: Snoke Deena B. , Mahler Connor A. , Angelotti Austin , Cole Rachel M. , Sparagna Genevieve C. , Baskin Kedryn K. , Belury Martha A.
Published: December 2022

Cardiolipin (CL) is a phospholipid unique to the inner mitochondrial membrane that supports respiratory chain structure and function and is demonstrated to be influenced by types of dietary fats. However, the influence of dietary fat on CL species and how this best supports mitochondrial function in brown adipose tissue (BAT), which exhibits an alternative method of energy utilization through the uncoupling of the mitochondrial proton gradient to generate heat, is not well understood. Therefore, the aim of our study was to evaluate metabolic parameters, interscapular BAT CL quantity, species, and mitochondrial function in mice consuming isocaloric moderate-fat diets with either lard (LD; similar fatty acid profile to western dietary patterns) or safflower oil high in linoleic acid (SO), shown to be metabolically favorable in large clinical meta-analyses. Mice fed the SO diet exhibited decreased adiposity, improved insulin sensitivity, and enrichment of LA-containing CL species in BAT CL. Furthermore, mice fed the SO diet exhibit higher levels of OXPHOS complex proteins and increased oxygen consumption in BAT. Our findings demonstrate that dietary consumption of LA-rich oil improves metabolic parameters, increases LA-containing CL species, and improves BAT function when compared to the consumption of lard in mice during diet-induced weight gain.
Considerations for Applying Route-to-Route Extrapolation to Assess the Safety of Oral Exposure to Substances
Authors: Kabadi Shruti V. , Fisher Jeffrey , Hung Benjamin , Aungst Jason
Published: December 2022

The safety evaluation of oral exposure to substances, such as food ingredients, additives, and their constituents, relies primarily on a careful evaluation and analysis of data from oral toxicity studies. When relevant oral toxicity studies are unavailable or may have significant data gaps that make them inadequate for use in safety evaluations, data from non-oral toxicity studies in animals, such as studies on inhalation, dermal exposure, etc., might be used in support of or in place of oral toxicity studies through route-to-route (R-t-R) extrapolation. R-t-R extrapolation is applied on a case-by-case basis as it requires attention to and comparison of substance-specific toxicokinetic (TK) and toxicodynamic (TD) data for oral and non-oral exposure routes. This article provides a commentary on the utility of R-t-R extrapolation to assess the safety of oral exposure to substances, with an emphasis on the relevance of TK and systemic toxicity data.
Metatranscriptomic Analysis Reveals Disordered Alterations in Oropharyngeal Microbiome during the Infection and Clearance Processes of SARS-CoV-2: A Warning for Secondary Infections
Authors: Zhou Yongzhao , Lu Sifen , Wei Xiaozhen , Hu Ya , Li Honghao , Wang Jing , Lin Yifei , Li Mengjiao , Wang Minjin , Ma Jinmin , Zhu Zhongyi , Yang Shengying , Ying Binwu , Zhang Wengeng , Chen Bojiang , Li Weimin
Published: December 2022

This study was conducted to investigate oropharyngeal microbiota alterations during the progression of coronavirus disease 2019 (COVID-19) by analyzing these alterations during the infection and clearance processes of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The diagnosis of COVID-19 was confirmed by using positive SARS-CoV-2 quantitative reverse transcription polymerase chain reaction (RT-qPCR). The alterations in abundance, diversity, and potential function of the oropharyngeal microbiome were identified using metatranscriptomic sequencing analyses of oropharyngeal swab specimens from 47 patients with COVID-19 (within a week after diagnosis and within two months after recovery from COVID-19) and 40 healthy individuals. As a result, in the infection process of SARS-CoV-2, compared to the healthy individuals, the relative abundances of Prevotella, Aspergillus, and Epstein–Barr virus were elevated; the alpha diversity was decreased; the beta diversity was disordered; the relative abundance of Gram-negative bacteria was increased; and the relative abundance of Gram-positive bacteria was decreased. After the clearance of SARS-CoV-2, compared to the healthy individuals and patients with COVID-19, the above disordered alterations persisted in the patients who had recovered from COVID-19 and did not return to the normal level observed in the healthy individuals. Additionally, the expressions of several antibiotic resistance genes (especially multi-drug resistance, glycopeptide, and tetracycline) in the patients with COVID-19 were higher than those in the healthy individuals. After SARS-CoV-2 was cleared, the expressions of these genes in the patients who had recovered from COVID-19 were lower than those in the patients with COVID-19, and they were different from those in the healthy individuals. In conclusion, our findings provide evidence that potential secondary infections with oropharyngeal bacteria, fungi, and viruses in patients who have recovered from COVID-19 should not be ignored; this evidence also highlights the clinical significance of the oropharyngeal microbiome in the early prevention of potential secondary infections of COVID-19 and suggests that it is imperative to choose appropriate antibiotics for subsequent bacterial secondary infection in patients with COVID-19.
Inhibition of PDE10A in a New Rat Model of Severe Dopamine Depletion Suggests New Approach to Non-Dopamine Parkinson’s Disease Therapy
Authors: Sukhanov Ilya , Dorotenko Artem , Fesenko Zoia , Savchenko Artem , Efimova Evgeniya V. , Mor Mikael S. , Belozertseva Irina V. , Sotnikova Tatyana D. , Gainetdinov Raul R.
Published: December 2022

Parkinson’s disease is the second most common neurodegenerative pathology. Due to the limitations of existing therapeutic approaches, novel anti-parkinsonian medicines with non-dopamine mechanisms of action are clearly needed. One of the promising pharmacological targets for anti-Parkinson drug development is phosphodiesterase (PDE) 10A. The stimulating motor effects of PDE10A inhibition were detected only under the conditions of partial dopamine depletion. The results raise the question of whether PDE10A inhibitors are able to restore locomotor activity when dopamine levels are very low. To address this issue, we (1) developed and validated the rat model of acute severe dopamine deficiency and (2) tested the action of PDE10A inhibitor MP-10 in this model. All experiments were performed in dopamine transporter knockout (DAT-KO) rats. A tyrosine hydroxylase inhibitor, α-Methyl-DL-tyrosine (αMPT), was used as an agent to cause extreme dopamine deficiency. In vivo tests included estimation of locomotor activity and catalepsy levels in the bar test. Additionally, we evaluated the tissue content of dopamine in brain samples by HPLC analysis. The acute administration of αMPT to DAT-KO rats caused severe depletion of dopamine, immobility, and catalepsy (Dopamine-Deficient DAT-KO (DDD) rats). As expected, treatment with the L-DOPA and carbidopa combination restored the motor functions of DDD rats. Strikingly, administration of MP-10 also fully reversed immobility and catalepsy in DDD rats. According to neurochemical studies, the action of MP-10, in contrast to L-DOPA + carbidopa, seems to be dopamine-independent. These observations indicate that targeting PDE10A may represent a new promising approach in the development of non-dopamine therapies for Parkinson’s disease.
Development of a Clinical Prediction Rule for Treatment Success with Transcranial Direct Current Stimulation for Knee Osteoarthritis Pain: A Secondary Analysis of a Double-Blind Randomized Controlled Trial
Authors: Teixeira Paulo E. P. , Tavares Daniela R. B. , Pacheco-Barrios Kevin , Branco Luis Castelo , Slawka Eric , Keysor Julie , Trevisani Virginia F. M. , Gross Doug K , Fregni Felipe
Published: December 2022

The study’s objective was to develop a clinical prediction rule that predicts a clinically significant analgesic effect on chronic knee osteoarthritis pain after transcranial direct current stimulation treatment. This is a secondary analysis from a double-blind randomized controlled trial. Data from 51 individuals with chronic knee osteoarthritis pain and an impaired descending pain inhibitory system were used. The intervention comprised a 15-session protocol of anodal primary motor cortex transcranial direct current stimulation. Treatment success was defined by the Western Ontario and McMaster Universities’ Osteoarthritis Index pain subscale. Accuracy statistics were calculated for each potential predictor and for the final model. The final logistic regression model was statistically significant (p < 0.01) and comprised five physical and psychosocial predictor variables that together yielded a positive likelihood ratio of 14.40 (95% CI: 3.66–56.69) and an 85% (95%CI: 60–96%) post-test probability of success. This is the first clinical prediction rule proposed for transcranial direct current stimulation in patients with chronic pain. The model underscores the importance of both physical and psychosocial factors as predictors of the analgesic response to transcranial direct current stimulation treatment. Validation of the proposed clinical prediction rule should be performed in other datasets.
Combined Usage of MDK Inhibitor Augments Interferon-γ Anti-Tumor Activity in the SKOV3 Human Ovarian Cancer Cell Line
Authors: Liu Qun , Tan Jingyu , Zhao Zhenguo , Li Ruijun , Zheng Luyu , Chen Xiangyu , Li Lina , Dong Xichen , Wen Tao , Liu Jian
Published: December 2022

Ovarian cancer (OC) is a particularly lethal disease due to intratumoral heterogeneity, resistance to traditional chemotherapy, and poor response to targeted therapy and immunotherapy. Interferon-γ (IFN-γ) is an attractive therapeutic cytokine, with positive responses achieved in multiple OC clinical trials. However, clinical application of IFN-γ in OC is still hindered, due to the severe toxicity when used at higher levels, as well as the considerable pro-metastatic adverse effect when used at lower levels. Thus, an effective combined intervention is needed to enhance the anti-tumor efficacy of IFN-γ and to suppress the IFN-γ-induced metastasis. Here, we uncovered that OC cells develop an adaptive strategy by upregulating midkine (MDK) to counteract the IFN-γ-induced anti-tumor activity and to fuel IFN-γ-induced metastasis. We showed that MDK is a critical downstream target of IFN-γ in OC, and that this regulation acts in a dose-dependent manner and is mediated by STAT1. Gain-of-function studies showed that MDK overexpression promotes cell proliferation and metastasis in OC, indicating that IFN-γ-activated MDK may antagonize IFN-γ in inhibiting OC proliferation but synergize IFN-γ in promoting OC metastasis. Subsequently, we assessed the influence of MDK inhibition on IFN-γ-induced anti-proliferation and pro-metastasis effects using an MDK inhibitor (iMDK), and we found that MDK inhibition robustly enhanced IFN-γ-induced growth inhibition (all CIs < 0.1) and reversed IFN-γ-driven epithelial-to-mesenchymal transition (EMT) and metastasis in OC in vitro. Collectively, these data identify an IFN-γ responsive protein, MDK, in counteracting anti-proliferation while endowing the pro-metastatic role of IFN-γ in cancer treatment, and we therefore propose the combined utilization of the MDK inhibitor in IFN-γ-based therapies in future OC treatment.
Electrochemical Aptasensor Developed Using Two-Electrode Setup and Three-Electrode Setup: Comprising Their Current Range in Context of Dengue Virus Determination
Authors: Hasan Mohd. Rahil , Sharma Pradakshina , Shaikh Shifa , Singh Saumitra , Pilloton Roberto , Narang Jagriti
Published: December 2022

We present, for the very first time, the fabrication and electrochemical characterization of a paper-based experimental platform for dengue virus analysis. The paper-based device incorporates a screen-printing technology with the help of black carbon conductive ink. The paper-based device utilizes two styles of electrode setups, i.e., the two-electrode system and three-electrode system, and both setups effectively detected the dengue virus with an LOD of 0.1 µg/mL; however, these paper electrodes exhibit various current ranges, and the created sensor was encompassed and compared in this research based on current response. It is observed that the three-electrode system has a substantially higher current range, ranging from 55.53 µA to 322.21 µA, as compared to the two-electrode system, which has a current range of 0.85 µA to 4.54 µA. According to this study, the three-electrode system displayed a good range of current amplification that is roughly 50 times higher than the two-electrode system, which had a weak current response. As a result, the three-electrode method has emerged as a viable option for the very sensitive detection of the dengue virus, as well as for the diagnosis of other diseases.
Improvement of Seed-Mediated Growth of Gold Nanoparticle Labels for DNA Membrane-Based Assays
Authors: Presnova Galina V. , Zhdanov Gleb A. , Filatova Luibov Yu. , Ulyashova Mariya M. , Presnov Denis E. , Rubtsova Maya Yu.
Published: December 2022

Gold nanoparticles (AuNPs) are popular labels for colorimetric detection of various analytes, involving proteins, nucleic acids, viruses, and whole cells because of their outstanding optical properties, inertness, and modification variability. In this work, we present an improved approach for enhancement of color intensity for DNA membrane microarrays based on seed-mediated growth of AuNP labels. Biotin-labeled DNA is hybridized with capture oligonucleotide probes immobilized on the microarrays. Then biotin is revealed by a streptavidin–AuNP conjugate followed by the detection of AuNPs. Optimization of seed-mediated enlargement of AuNPs by the reduction of tetrachloroauric acid with hydroxylamine made it possible to change the coloring of specific spots on the microarrays from pink to a more contrasting black with minor background staining. Mean size of the resulting AuNPs was four times larger than before the enhancement. Adjusting the pH of HAuCl4 solution to 3.5 and use of a large excess of hydroxylamine increased the signal/background ratio by several times. The method’s applicability was demonstrated for quantification of a short oligonucleotide of 19 bases and full-length TEM-type β-lactamase genes of 860 bp responsible for the development of bacterial resistance against β-lactam antibiotics. Improved protocol for AuNP enlargement may be further transferred to any other membrane-based assays of nucleic acids with both instrumental and visual colorimetric detection.
Exploring the Social Trend Indications of Utilizing E-Commerce during and after COVID-19’s Hit
Authors: Fakieh Bahjat , Happonen Ari
Published: December 2022

COVID-19 is a major global crisis affecter, changing global norms and societal behavioral models. Many companies have faced existential crises, but on the other hand, businesses that were and are helping others to boost digitalization, ICT and software solutions deployment, remote communications integration, e-commerce & e-services, and so on, have boosted their businesses, as people shifted online during the global lockdown and international travel restrictions. Our work explores the trend of e-commerce and e-services utilization during the ease of restrictions and the social distancing period to forecast the trend continuation patterns after the pandemic. An online survey was conducted and targeted individuals in Saudi Arabia and Egypt, resulting in 155 participants. The data were analyzed from four perspectives: demographics, COVID-19 health impact, trend analysis, and regression analysis. The results indicate heavy utilization of e-commerce and e-services during the global movement restrictions and travel bans. This trend has, however, significantly reduced during the ease of restrictions and social distancing period. Utilizing e-commerce and e-services in Saudi Arabia and Egypt, based on the research data, is positively correlated to the outbreak conditions. On the other hand, current data still does not give clear indications, and this pattern is going to be mostly, partly, or not at all permanent now as societies are returning to mostly a free movement of people and marginally restricted social distancing times.
Research on the Development and Joint Improvement of Ceramsite Lightweight High-Titanium Heavy Slag Concrete Precast Composite Slab
Authors: Sun Jinkun , Li Rita Yi Man , Jiao Tao , Wang Senping , Deng Chenxi , Zeng Liyun
Published: December 2022

Despite the continuous improvement in the research and development of concrete precast composite slab technology, problems like easy cracks and excessive weight at the joints remain. In this study, high-titanium heavy slag was mixed with different kinds of ceramsite to prepare ceramsite lightweight high-titanium heavy slag concrete. The joint of the composite slab was optimized to develop a novel ceramsite lightweight high-titanium heavy slag concrete precast composite slab, hereinafter referred to as “CLHCPCS”. Two CLHCPCS and one ordinary concrete composite slab were prepared. This study analyzed the effects of new materials and improved joints on the flexural capacity and crack resistance of CLHCPCS. It concluded that the density of high-titanium heavy slag concrete with shale ceramsite decreased by 12.0%, and the density of high-titanium heavy slag concrete with fly ash ceramsite decreased by 10.6%. At a 30% dosage of fly ash ceramsite, the compressive strength and splitting tensile strength of concrete reached the maximum. At a 20% dosage of shale ceramsite, the mechanical properties were optimal. Finally, fly ash ceramsite was selected as part coarse aggregate of CLHCPCS. CLHCPCS 1 and 2 demonstrated superior ultimate bearing capacity and crack resistance than ordinary concrete composite slab DBS1, with its ultimate bending capacity test value higher than the average value of ordinary concrete composite slab. ANSYS established the joint model of CLHCPCS for a bending simulation test. The stress and strain distribution of the model and the ultimate bending capacity under the plastic line method were obtained, consistent with theory and experimental analysis results.
Splanchnic Vein Thrombosis in Myeloproliferative Neoplasms: Treatment Considerations and Unmet Needs
Authors: Liu Angela , Naymagon Leonard , Tremblay Douglas
Published: December 2022

Patients who develop splanchnic vein thrombosis (SVT) in the setting of a myeloproliferative neoplasm (MPN) are at risk for complications including portal hypertension, bleeding, thrombosis, and death. Prompt multidisciplinary treatment is thus necessary to prevent long-term sequelae. However, optimal management strategies are not well established due to a paucity of data. In this review, we very briefly discuss the epidemiology, pathophysiology, and prognosis of MPN-SVT and then more comprehensively explore treatment considerations of MPN-SVT, including anticoagulation, endovascular/surgical intervention, and cytoreductive therapy. We will also highlight current gaps in our knowledge of MPN-SVT and conclude by suggesting future directions to optimize the treatment of MPN-SVT and improve outcomes.
Investigation of CeO2, MoO3, and Ce2(MoO4)3, Synthesized by the Pechini Method, as Catalysts for Fructose Conversion
Authors: Pryston Dhara Beatriz de Amorim , Martins Thatiane Veríssimo dos Santos , Vasconcelos Júnior Jailton Alves de , Avelino Débora Olimpio da Silva , Meneghetti Mario Roberto , Meneghetti Simoni Margareti Plentz
Published: December 2022

Cerium oxide (Ce100), molybdenum oxide (Mo100), and a material containing Ce and Mo (CeMo) were synthesized by the Pechini method, using glycerol as a polyol. These materials were applied for fructose conversion in an aqueous medium. The characterization results show the formation of cerium molybdate (Ce2(MoO4)3) for CeMo. Ce100 presented good thermal stability, and Mo100 sublimation of MoO3 and polymolybdates was verified. CeMo exhibited a mass loss of 19%, associated with the sublimation of MoO3 and polymolybdate species. Additionally, the existence of Bronsted and Lewis acid sites was confirmed, and the addition of Mo to Ce was an efficient strategy to increase the acidity. Regarding the catalytic activity (150 °C and 0.5 to 6 h), Ce100 exhibited low conversions and high selectivity to 5-hydroxymethylfurfural (5-HMF). For Mo100, high conversions, with a significant formation of insoluble materials, were detected. For CeMo, beyond the high activity, a lower formation of insoluble materials was noted. In this case, selectivity toward products from the retro–aldolic route and 5-HMF were obtained. These results indicate that the main factor influencing fructose conversion is an adequate combination of the acid sites. Recycling experiments were carried out, and stability was observed for four cycles, confirming the robustness of this system.
Blue Rubber Bleb Nevus Syndrome (BRBNS): A Rare Cause of Refractory Anemia in Children
Authors: Chen Liang-Chun , Yeung Chun-Yan , Chang Chen-Wang , Lee Hung-Chang , Chan Wai-Tao , Jiang Chuen-Bin , Chang Szu-Wen
Published: December 2022

Refractory anemia is not uncommon in pediatric patients, and anemia caused by gastrointestinal tract bleeding should always be kept in mind. Aside from infection or intestinal malrotation related bleeding, vascular malformation should also be considered. Blue rubber bleb nevus syndrome (BRBNS) is a rare vascular disorder consisting of multiple venous malformations. Lack of experience in pediatric BRBNS might lead to delayed diagnosis or misdiagnosis. Herein, we report a case of an eleven-year-old boy with recurrent pallor appearance and weakness diagnosed with BRBNS. After a thorough examination, he was treated with endoscopic polypectomy, and further iron supplements and folic acid. He is now under regular follow-up at our outpatient department. No complication is noted for six months. BRBNS is a rare venous malformation syndrome that mostly involves skin and the gastrointestinal tract. Multidisciplinary approach should be arranged for diagnosis and management. Up to date, no consensus for BRBNS treatment has been reached. Management usually depends on clinical symptoms and severity of damage of involved organs. The options of treatment include conservative, medical, endoscopic, and surgical management.