bluuebunny/crossref_metadata_2025 huggingface dataset:
Directionally Illuminated Autostereoscopy with Seamless Viewpoints for Multi-Viewers
Authors: Zhang Aiqin , Chen Xuehao , Wang Jiahui , He Yong , Zhou Jianying
Published: March 2024

Autostereoscopy is usually perceived at finite viewpoints that result from the separated pixel array of a display system. With directionally illuminated autostereoscopy, the separation of the illumination channel from the image channel provides extra flexibility in optimizing the performance of autostereoscopy. This work demonstrates that by taking advantage of illumination freedom, seamless viewpoints in the sweet viewing region, where the ghosting does not cause significant discomfort, are realized. This realization is based on illuminating the screen with a polyline array of light emitting diodes (LEDs), and continuous viewpoints are generated through independent variation in the radiance of each individual LED column. This new method is implemented in the directionally illuminated display for both single and multiple viewers, proving its effectiveness as a valuable technique for achieving a high-quality and high-resolution autostereoscopic display with seamless viewpoints.
Therapy of Mycobacterium abscessus Infections in Solid Organ Transplant Patients
Authors: Osman Lubna , Lopez Christopher , Natori Yoichiro , Anjan Shweta , Bini Viotti Julia , Simkins Jacques
Published: March 2024

Mycobacterium abscessus complex (MABC), a rapidly growing Mycobacterium, is one of the most common causes of non-tuberculous mycobacteria (NTM) infections in the United States of America, and it has been associated with a wide spectrum of infections in immunocompetent and immunosuppressed individuals. Eradicating MABC is very challenging, even with prolonged combination therapies. The management of MABC infections in solid organ transplant (SOT) patients is usually complex given their net state of immunosuppression, associated comorbidities, and potential drug–drug interactions, among other things. In this manuscript, we discussed the antimicrobial management of pulmonary and extrapulmonary MABC infections. In addition, we reviewed promising novel therapies such as clofazimine, omadacycline, bedaquiline, and inhaled tigecycline that could join the existing antimicrobial armamentarium to fight this infection associated with significant morbidity and mortality. However, further studies are needed, especially among the immunocompromised host.
Identification and Pathogenicity of Fusarium Fungi Associated with Dry Rot of Potato Tubers
Authors: Gavrilova Olga , Orina Aleksandra , Trubin Ilya , Gagkaeva Tatiana
Published: March 2024

Dry rot of potato tubers is a harmful disease caused by species of the Fusarium genus. Studies on the composition and features of Fusarium spp. that cause the disease in Russia are limited. Thirty-one Fusarium strains belonging to the F. sambucinum species complex (FSAMSC) and F. solani species complex (FSSC) were accurately identified using multilocus phylogenetic analysis of the tef and rpb2 loci, and their physiological characteristics were studied in detail. As a result, 21 strains of F. sambucinum s. str. and 1 strain of F. venenatum within the FSAMSC were identified. Among the analyzed strains within the FSSC, one strain of F. mori, four strains of F. noneumartii, and two strains of both F. stercicola and F. vanettenii species were identified. This is the first record of F. mori on potato as a novel host plant, and the first detection of F. noneumartii and F. stercicola species in Russia. The clear optimal temperature for the growth of the strains belonging to FSAMSC was noted to be 25 °C, with a growth rate of 11.6–15.0 mm/day, whereas, for the strains belonging to FSSC, the optimal temperature range was between 25 and 30 °C, with a growth rate of 5.5–14.1 mm/day. The distinctive ability of F. sambucinum strains to grow at 5 °C has been demonstrated. All analyzed Fusarium strains were pathogenic to potato cv. Gala and caused extensive damage of the tuber tissue at an incubation temperature of 23 °C for one month. Among the fungi belonging to the FSAMSC, the F. sambucinum strains were more aggressive and caused 23.9 ± 2.2 mm of necrosis in the tubers on average compared to the F. venenatum strain—17.7 ± 1.2 mm. Among the fungi belonging to the FSSC, the F. noneumartii strains were the most aggressive and caused 32.2 ± 0.8 mm of necrosis on average. The aggressiveness of the F. mori, F. stercicola, and especially the F. vanettenii strains was significantly lower: the average sizes of damage were 17.5 ± 0.5 mm, 17.2 ± 0.2 mm, and 12.5 ± 1.7 mm, respectively. At an incubation temperature of 5 °C, only the F. sambucinum strains caused tuber necroses in the range of 6.7 ± 0.5–15.9 ± 0.8 mm.
Effects of Six Natural Compounds and Their Derivatives on the Control of Coccidiosis in Chickens
Authors: Hou Yue , Han Bo , Lin Zehua , Liu Qizheng , Liu Zhenhe , Si Hongbin , Hu Dandan
Published: March 2024

Chicken coccidiosis costs the poultry industry over GBP 10 billion per year. The main method of preventing and controlling coccidiosis in chickens continues to be the use of drugs. Unfortunately, the prevalence of drug resistance in the field reduces or even eliminates the effectiveness of drugs, and drug residues in the food supply chain can also can be harmful to humans. Therefore, safe and effective anticoccidial drugs are urgently needed. Natural products have many advantages such as being safe, effective and inexpensive and are a sustainable way to control coccidiosis. In this study, the anticoccidial effects of six natural compounds were tested by Eimeria tenella infection. Oocyst production, cecum lesion, body weight gain, feed conversion ratio, and intestinal microbiota were measured. The results showed that nerolidol had a moderate effect on maintaining both body weight gain and feed conversion ratio. Silymarin and dihydroartemisinin showed significant anticoccidial effects by reducing total oocyst output. Dihydroartemisinin also significantly reduced the cecum lesion caused by Eimeria infection, but this compound may be toxic to the host at such informed doses because it decreases growth and survival rates. In addition, both silymarin and dihydroartemisinin partly restored the microbiota after challenge. This indicates that silymarin, dihydroartemisinin, and nerolidol are effective in the control of chicken coccidiosis. Our data provide basic knowledge about the anticoccidial effects of such natural compounds/derivates.
Effects of Salmonella Typhimurium Infection on the Gut Microbiota of Cherry Valley Meat Ducks
Authors: Zheng Yue , Pan Xue , Hou Jialei , Shi Wenchong , Sun Shuhong , Song Mengze , Gao Zheng
Published: March 2024

Salmonella infection causes serious economic losses, threatens food safety, and is one of the most important diseases threatening meat duck farming. The gut microbiome is critical in providing resistance against colonization by exogenous microorganisms. Studying the relationship between Salmonella and gut microbiota can help us better understand the threat of the pathogenic mechanism of Salmonella and provide a more scientific theoretical basis for its prevention and treatment. This study uses Salmonella Typhimurium as the research object and Cherry Valley meat duck as the model with which to study the impact of Salmonella infection on ducks. In this field trial, 2 × 108 CFUs Salmonella Typhimurium were administered to 3-day-old ducks. After infection, duck viscera were collected to detect the colonization of Salmonella, and cecal contents were collected to analyze the changes in gut microbiota. The results show that Salmonella Typhimurium can colonize ducks three days after infection and alter the gut microbiota composition, mainly by increasing the abundance of Ruminococcaceae and Lachnospiraceae. In conclusion, Salmonella Typhimurium infection significantly alters the intestinal microbiota of ducks and poses a serious public health risk.
The Performance of a Modified Anode Using a Combination of Kaolin and Graphite Nanoparticles in Microbial Fuel Cells
Authors: Hirsch Lea Ouaknin , Gandu Bharath , Chiliveru Abhishiktha , Amar Dubrovin Irina , Rozenfeld Shmuel , Schechter Alex , Cahan Rivka
Published: March 2024

The bacterial anode in microbial fuel cells was modified by increasing the biofilm’s adhesion to the anode material using kaolin and graphite nanoparticles. The MFCs were inoculated with G. sulfurreducens, kaolin (12.5 g·L−1), and three different concentrations of graphite (0.25, 1.25, and 2.5 g·L−1). The modified anode with the graphite nanoparticles (1.25 g·L−1) showed the highest electroactivity and biofilm viability. A potential of 0.59, 0.45, and 0.23 V and a power density of 0.54 W·m−2, 0.3 W·m−2, and 0.2 W·m−2 were obtained by the MFCs based on kaolin–graphite nanoparticles, kaolin, and bare anodes, respectively. The kaolin–graphite anode exhibited the highest Coulombic efficiency (21%) compared with the kaolin (17%) and the bare (14%) anodes. Scanning electron microscopy and confocal laser scanning microscopy revealed a large amount of biofilm on the kaolin–graphite anode. We assume that the graphite nanoparticles increased the charge transfer between the bacteria that are in the biofilm and are far from the anode material. The addition of kaolin and graphite nanoparticles increased the attachment of several bacteria. Thus, for MFCs that are fed with wastewater, the modified anode should be prepared with a pure culture of G. sulfurreducens before adding wastewater that includes non-exoelectrogenic bacteria.
One Step Forwards in Knowledge of Blossom Blight Brown Rot Disease: Monilinia spp. SSR Marker Database
Authors: Antanynienė Raminta , Stanys Vidmantas , Frercks Birutė
Published: March 2024

A freely available Monilinia spp. marker database was created, containing microsatellite (SSR) data of the three most essential European fungal pathogens: M. fructigena, M. laxa, and M. fructicola. These pathogens cause brown rot blossom blight. Microsatellites were identified using the bioinformatics tool Genome-wide Microsatellite Analyzing Toward Application (GMATA). The database provides information about SSR markers: forward and reverse sequences of the primers, fragment sizes, SSR motifs (and repeats), and the exact locations with the coordinates in the reference genome. This database currently contains information about 39,216 SSR motifs and 26,366 markers. In total, eight primers generated in silico were validated experimentally and they are marked in the database. All scientists can join this collaboration by adding their experimental data. This database is the initial start of organizing Monilinia spp. molecular data worldwide and, in the future, it could be extended by adding more molecular and genomic information.
Leaching of Nickel and Cobalt from a Mixed Nickel-Cobalt Hydroxide Precipitate Using Organic Acids
Authors: Hussaini Shokrullah , Tita Angela Manka , Kursunoglu Sait , Kaya Muammer , Chu Pengbo
Published: March 2024

Nickel (Ni) and cobalt (Co) are strategic metals that have found applications in a wide range of metallurgical and industrial uses. In this study, the dissolution of a mixed nickel–cobalt hydroxide precipitate using organic acids (citric, oxalic, and malic acid) was investigated. Citric acid was found to be the best leaching agent yielding the following dissolution rates: 91.2% Ni, 86.8% Co, and 90.8% Mn. Oxalic acid resulted in low dissolution, which is likely due to the formation of insoluble metal oxalates. The impact of acid concentration, leaching time, and temperature on metal dissolution was systematically examined. The optimal dissolution conditions were identified as 0.5 M citric acid at 30 °C for 30 min, utilizing a 1/20 solid/liquid ratio and a stirring speed of 400 revolutions per minute (rpm). The attempt to use oxidants, such as potassium permanganate (KMnO4) and hydrogen peroxide (H2O2), to achieve selective dissolution in an organic acid environment was not successful, which was different from that in the sulfuric acid case. As for the leaching kinetics in the organic acids, it seems that the leaching of Ni correlates with the Shrinking Core Model, specifically regarding porous-layer diffusion control. Based on the experimental results, the activation energy for the leaching of Ni was estimated to be 3.1 kJ/mol.
Use and Recovery of Extractive Waste and Tailings for Sustainable Raw Materials Supply
Authors: Mancini Susanna , Casale Marco , Tazzini Antonio , Dino Giovanna Antonella
Published: March 2024

Extractive waste (EW), including tailings, is produced in large quantities during mining activities. In recent years, the linear economic model (“take-use-and-throw” approach) has been replaced by a circular approach, emphasizing the sustainable use and recovery of EW. The development of innovative protocols, such as Best Available Techniques (BATs), which aim at the technological and process improvement of more sustainable mining activities and at the production of renewable, highly performing green materials, has led to technological advancements, expertise in sustainability, and a reduced ecological footprint, potentially causing positive economic and social impacts and reducing environmental ones. Extractive waste and tailings, if suitably characterized, can be used to improve and make sustainable the works connected to the management of mining activities. The qualitative–quantitative characterization of EW is essential for subsequent reuse and for assessing the risk to human health and the extent of environmental impacts in the various matrices. The application areas vary according to the type of waste and mining tailings, the morphological characteristics of the deposits, and the geological, geomorphological, and logistic context of the area. Integrated protocols for sustainable EW exploitation and positive impacts on the economic, environmental, and technological/social level are analyzed. The present paper aims at providing an overview of challenges and potentialities connected to extractive waste (EW) management and potential exploitation to recover raw materials (RM), critical raw materials (CRM), and secondary raw materials (SRM).
Selective Generation of Aldimine and Ketimine Tautomers of the Schiff Base Condensates of Amino Acids with Imidazole Aldehydes or of Imidazole Methanamines with Pyruvates—Isomeric Control with 2- vs. 4-Substituted Imidazoles
Authors: Brewer Greg , Brewer Cynthia , Butcher Raymond J. , Zavalij Peter
Published: March 2024

The Schiff base condensation of 5-methyl-4-imidazole carboxaldehyde, 5Me4ImCHO, and the anion of an amino acid, H2N-CH(R)CO2− (R = -CH3, -CH(CH3)2 and -CH2CH(CH3)2), gives the aldimine tautomer, Im-CH=N-CH(R)CO2−, while that of 5-methylimidazole-4-methanamine, 5MeIm-4-CH2NH2, with a 2-oxocarboxylate anion, R-C(O)-CO2−, gives the isomeric ketimine tautomer, Im-CH2-N=C(R)CO2−. All are isolated as the neutral nickel(II) complexes, NiL2, and are characterized by single crystal structure determination, IR, and positive ion ESI MS. In the cases of the 4 substituted imidazoles, either 5MeIm-4-CHO or 5MeIm-4-CH2NH2, both the aldimine and ketimine complexes are isolated cleanly with no evidence of an equilibrium between the two tautomers under the experimental conditions. The aldimines are blue while the tautomeric ketimines are green. In contrast, for the 2-substituted imidazoles, with either Im-2-CHO or Im-2-CH2NH2, the isolated product from the Schiff base condensation is the ketimine, which in the solid is green, as observed for the 4-isomer. These results suggest that for the 2-substituted imidazoles, there is a facile equilibrium between the aldimine and ketimine tautomers, and that the ketimine form is the thermodynamically favored tautomer. The aldimine tautomers of the 4-substituted imidazoles have three stereogenic centers, the nickel (Δ or Ʌ) and the two alpha carbon atoms (R or S). The observed pair of enantiomers is the ɅRR/ΔSS enantiomeric pair, suggesting that this pair is lower in energy than the others and that this is in general the preferred chiral correlation in these complexes.
Synthetic Methods and Pharmacological Potentials of Triazolothiadiazines: A Review
Authors: Mostafa Mohamed S. , Radini Ibrahim Ali M. , El-Rahman Naglaa M. Abd , Khidre Rizk E.
Published: March 2024

This review article examines the synthetic pathways for triazolothiadiazine derivatives, such as triazolo[3,4-b]thiadiazines, triazolo[5,1-b]thiadiazines, and triazolo[4,3-c]thiadiazines, originating from triazole derivatives, thiadiazine derivatives, or thiocarbohydrazide. The triazolothiadiazine derivatives exhibit several biological actions, including antibacterial, anticancer, antiviral, antiproliferative, analgesic, anti-inflammatory, and antioxidant properties. The review article aims to assist researchers in creating new biologically active compounds for designing target-oriented triazolothiadiazine-based medicines to treat multifunctional disorders.
Chemoenzymatic Synthesis of Selegiline: An Imine Reductase-Catalyzed Approach
Authors: Hu Yuliang , Bao Jinping , Tang Dongyu , Gao Shushan , Wang Fei , Ding Zhongtao , Cui Chengsen
Published: March 2024

(R)-Homobenzylic amines are key structural motifs present in (R)-selegiline, a drug indicated for the treatment of early-stage Parkinson’s disease. Herein, we report a new short chemoenzymatic approach (in 2 steps) towards the synthesis of (R)-selegiline via stereoselective biocatalytic reductive amination as the key step. The imine reductase IR36-M5 mutant showed high conversion (97%) and stereoselectivity (97%) toward the phenylacetone and propargyl amine substrates, offering valuable biocatalysts for synthesizing alkylated homobenzylic amines.
Enhanced Photovoltaic Performance of Asymmetrical Benzo Dithiophene Homopolymer Donor Materials in Nonfullerene Acceptor-Based Organic Photovoltaics
Authors: Xu Wei , Du Li , Du Zhengkun , He Wei , Li Hongxiang , Li Guojuan , Yang Cheng , Cheng Pei , Cao Zhong , Yu Donghong
Published: March 2024

Although much promising synthetic progress in conjugated polymer-based organic solar cells (OSCs) has resulted in significant improvement in power conversion efficiencies (PCEs) of from over 15 to >19.0% in the last five years, the sophisticated and complex reactions from at least two families’ monomers with remarkably different electron push–pull effects could still pose an unavoidable material burden for the commercialization of OSCs in the coming future. Therefore, the method of preparing a homopolymer from a sole monomer would significantly reduce the synthetic steps and costs in order to pave the way for the large-scale production of OSC materials. Therefore, alkylthio-thiophenyl-substituted benzo[1,2-b;4,5-b′]dithiophene (BDTTS) as the sole and key structural moiety with dihalogen and distannyl functional groups was designed and synthesized, respectively, in this study, for facile monomer syntheses and polymerizations to achieve three wide-bandgap homopolymer donors of BDTTS-alt-BDTT-Cl (P13), BDTTS-alt-BDTT (P15), and BDTTS (P14), respectively. The structural symmetry dependency on their physical, electrochemical, and optical properties, thin-film morphologies, and photovoltaic (PV) performance was investigated in detail. As a result, OSCs based on the asymmetric polymer P15, paired with BTP-eC9 as the electron acceptor, presented the best PV performance, with a PCE of 11.5%, a fill factor (FF) of 65.87%, and a short-circuit current (JSC) of 22.04 mA·cm−2, respectively. This PCE value is among the highest ones reported for BDT-type homopolymer donor-based OPVs, providing us with knowledge for obtaining promising PV performance from devices made of P15-like materials.
Controllable La Deficiency Engineering within Perovskite Oxides for Enhanced Overall Water Splitting
Authors: Xu Xiaohu , Guo Kaiwei , Yu Xinyue
Published: March 2024

Recently, perovskite (ABO3) nanomaterials have been widely explored as a class of versatile electrocatalysts for oxygen evolution reactions (OER) due to their remarkable compositional flexibility and structural tunability, but their poor electrical conductivity hinders hydrogen evolution reaction (HER) activity and further limits the large-scale application of perovskite oxide in overall water splitting (OWS). In this study, hollow-nanotube-structure LaxCo0.4Fe0.6O3−δ (x = 1.0, 0.9, and 0.8) perovskites with superior HER/OER activity were synthesized on nickel-iron alloy foam (denoted LaxCoFe/NFF) using hydrothermal with a subsequent calcination strategy. Among them, La0.9CoFe/NFF not only exhibited extraordinary HER electrocatalytic performance (160.5 mV@10 mA cm−2 and 241.0 mV@100 mA cm−2) and stability (20 h@10 mA cm−2), but also displayed significant OER electrocatalytic activity (234.7 mV@10 mA cm−2 and 296.1 mV@100 mA cm−2) and durability (20 h@10 mA cm−2), outperforming many recently reported HER/OER perovskite catalysts. The increase in oxygen vacancies caused by the introduction of La deficiency leads to the expansion of the lattice, which greatly accelerates the HER/OER process of La0.9CoFe/NFF. Additionally, the naturally porous skeleton can prevent catalysts from aggregating as well as delay the corrosion and dissolution of catalysts in the electrolyte under high applied potentials. Furthermore, the assembled two-electrode configuration, utilizing La0.9CoFe/NFF (cathode and anode) electrodes, only requires a low cell voltage of 1.573 V at 10 mA cm−2 for robust alkaline OWS, accompanied by remarkable durability over 20 h. This work provides inspiration for the design and preparation of high-performance and stable bifunctional perovskite electrocatalysts for OWS.
Microwave-Assisted Grafting of Coal onto Nitrogen-Doped Carbon Dots with a High Quantum Yield and Enhanced Photoluminescence Properties
Authors: Shi Chong , Wei Xian-Yong
Published: March 2024

The fluorescent nitrogen-doped carbon dots (N-CDs) were synthesized via a facile one-pot solvothermal process using coal (Jin 15 Anthracite and Shaerhu lignite) as raw materials and dimethyl formamide (DMF) as the solvent, employing a microwave pyrolysis method. This approach demonstrates remarkable efficacy in the development of nitrogen-doped carbon dots (N-CDs) with a high quantum yield (QY). The N-CDs prepared have strong photoluminescence properties. Moreover, the obtained N-CDs emit blue PL and are easily dispersed in polymethyl methacrylate (PMMA), preserving the inherent advantages of N-CDs and the PMMA matrix. The JN-CDs exhibit a high quantum yield (QY) of 49.5% and a production yield of 25.7%, respectively. In contrast, the SN-CDs demonstrate a quantum yield of 40% and a production yield of 35.1%. It is worth noting that the production yield and quantum yield of coal-based carbon dots are inversely related indices. The lower metamorphic degree of subbituminous coal favors an enhanced product yield, while the higher metamorphic degree of anthracite promotes an improved quantum yield in the product, which may be attributed to the presence of amorphous carbon within it. Consequently, we propose and discuss potential mechanisms underlying N-CD formation.
Rational Design of a Portable Chemometric-Assisted Voltammetric Sensor Based on Ion-Imprinted Polymeric Film for Co(II) Determination in Water
Authors: Di Masi Sabrina , Manrique Rodriguez Nelson Arturo , Costa Marco , De Benedetto Giuseppe Egidio , Malitesta Cosimino
Published: March 2024

Herein, chemometric-assisted synthesis of electrochemical sensors based on electropolymerised ion-imprinted polymeric (e-IIP) films was explored. Co(II)-IIPs sensors were prepared by performing electropolymerisation procedures of polymerisation mixtures comprising varying concentrations of an electroactive o-aminophenol (o-AP) monomer and Co(II) ions, respectively, according to the Taguchi L9 experimental design, exploiting the simultaneous evaluation of other controlled parameters during electrosynthesis. Each e-IIP developed from Taguchi runs was compared with the respective non-imprinted polymer (NIP) films and fitted according to Langmuir–Freudlich isotherms. Distinctive patterns of low and high-affinity films were screened based on the qualities and properties of the developed IIPs in terms of binding kinetics (KD), imprinting factor, and the heterogeneity index of produced cavities. These results can provide a generic protocol for chemometric-assisted synthesis of e-IIPs based on poly-o-AP, providing highly stable, reproducible, and high-affinity imprinted polymeric films for monitoring purposes.
Quantum Limit for the Emittance of Dirac Particles Carrying Orbital Angular Momentum
Authors: Curcio Alessandro , Cianchi Alessandro , Ferrario Massimo
Published: March 2024

In this article, we highlight that the interaction potential confining Dirac particles in a box must be invariant under the charge conjugation to avoid the Klein paradox, in which an infinite number of negative-energy particles are excited. Furthermore, we derive the quantization rules for a relativistic particle in a cylindrical box, which emulates the volume occupied by a beam of particles with a non-trivial aspect ratio. We apply our results to the evaluation of the quantum limit for emittance in particle accelerators. The developed theory allows the description of quantum beams carrying Orbital Angular Momentum (OAM). We demonstrate how the degeneracy pressure is such to increase the phase–space area of Dirac particles carrying OAM. The results dramatically differ from the classical evaluation of phase–space areas, that would foresee no increase in emittance for beams in a coherent state of OAM. We discuss the quantization of the phase–space cell’s area for single Dirac particles carrying OAM, and, finally, provide an interpretation of the beam entropy as the measure of how much the phase–space area occupied by the beam deviates from its quantum limit.
An Entomological Investigation during a Recent Rift Valley Fever Epizootic/Epidemic Reveals New Aspects of the Vectorial Transmission of the Virus in Madagascar
Authors: Tantely Luciano Michaël , Andriamandimby Soa Fy , Ambinintsoa Maminirina Fidelis , Raharinirina Manou Rominah , Rafisandratantsoa Jean Théophile , Ravalohery Jean-Pierre , Harimanana Aina , Ranoelison Nirina Nantenaina , Irinantenaina Judickaelle , Ankasitrahana Miamina Fidy , Ranoaritiana Dany Bakoly , Randrianasolo Laurence , Randremanana Rindra Vatosoa , Lacoste Vincent , Dussart Philippe , Girod Romain
Published: March 2024

A Rift Valley fever (RVF) outbreak occurred in at least five regions of Madagascar in 2021. The aim of this study was to provide an overview of the richness, abundance, ecology, and trophic preferences of mosquitoes in the Mananjary district and to investigate the distribution of mosquitoes that were RT-PCR-positive for RVFV. Three localities were prospected from 26 April to 4 May 2021, using light traps, BG-Sentinel traps baited with an artificial human odor, Muirhead-Thomson pit traps, and indoor pyrethroid spray catches. A total of 2806 mosquitoes belonging to at least 26 species were collected. Of 512 monospecific pools of mosquitoes tested with real-time RT-PCR, RVFV was detected in 37 pools representing 10 mosquito species. The RVFV-positive species were as follows: Aedes albopictus, Ae. argenteopunctatus, Anopheles coustani, An. gambiae s.l., An. mascarensis, An. squamosus/cydippis, Culex antennatus, Cx. decens, Cx. Tritaeniorhynchus, and Uranotaenia spp. Of the 450 tested engorged females, 78.7% had taken a blood meal on humans, 92.9% on cattle, and 71.6% had taken mixed (human–cattle) blood meals. This investigation suggests the potential role of mosquitoes in RVFV transmission within this epizootic/epidemic context and that the human populations at the three study sites were highly exposed to mosquitoes. Therefore, the use of impregnated mosquito nets as an appropriate prevention method is recommended.
Infections in Disorders of Immune Regulation
Authors: Thangaraj Abarna , Tyagi Reva , Suri Deepti , Gupta Sudhir
Published: March 2024

Primary immune regulatory disorders (PIRDs) constitute a spectrum of inborn errors of immunity (IEIs) that are primarily characterized by autoimmunity, lymphoproliferation, atopy, and malignancy. In PIRDs, infections are infrequent compared to other IEIs. While susceptibility to infection primarily stems from antibody deficiency, it is sometimes associated with additional innate immune and T or NK cell defects. The use of immunotherapy and chemotherapy further complicates the immune landscape, increasing the risk of diverse infections. Recurrent sinopulmonary infections, particularly bacterial infections such as those associated with staphylococcal and streptococcal organisms, are the most reported infectious manifestations. Predisposition to viral infections, especially Epstein–Barr virus (EBV)-inducing lymphoproliferation and malignancy, is also seen. Notably, mycobacterial and invasive fungal infections are rarely documented in these disorders. Knowledge about the spectrum of infections in these disorders would prevent diagnostic delays and prevent organ damage. This review delves into the infection profile specific to autoimmune lymphoproliferative syndrome (ALPS), Tregopathies, and syndromes with autoimmunity within the broader context of PIRD. Despite the critical importance of understanding the infectious aspects of these disorders, there remains a scarcity of comprehensive reports on this subject.
Timely, Cheap, or Risk-Free? The Effect of Regulation on the Price and Availability of New Drugs
Authors: Levaggi Laura , Levaggi Rosella
Published: March 2024

The high level of regulation of innovative drugs on the market, which is necessary to protect consumers, produces important effects on drug availability and innovation. In public healthcare systems, the need to curb prices comes from expenditure considerations. The aim of price regulation is to obtain a more equitable allocation of the value of an innovative drug between industries and patients (by reducing prices to make drugs more affordable), but it may also reduce access. (In the listing process, the industry may find it more convenient to limit commercialisation to profitable subgroups of patients.) Furthermore, with the advent of personalised medicine, there is another important dimension that has to be considered, namely, incentives to invest in drug personalisation. In this paper, we review and discuss the impact of different pricing rules on the expenditure and availability of new drugs.
Chemical Profile of Ocotea delicata (Lauraceae) Using Ultra High-Performance Liquid Chromatography–High-Resolution Mass Spectrometry–Global Natural Products Social Molecular Networking Workflow
Authors: da Silva Antonio Ananda , dos Santos Gustavo Ramalho Cardoso , Pereira Henrique Marcelo Gualberto , da Veiga-Junior Valdir Florêncio , Wiedemann Larissa Silveira Moreira
Published: March 2024

Ocotea, the largest genus in the Lauraceae family, encompasses numerous species of scientific interest. However, most Ocotea species have only been described morphologically. This study used an untargeted metabolomics workflow with UHPLC-HRMS and GNPS-FBMN to provide the first chemical evaluation of the polar specialized metabolites of O. delicata leaves. Leaves from three O. delicata specimens were extracted using ultrasound-assisted extraction with 70% ethanol. Among the examined samples, 44 metabolites, including alkaloids and flavonoids, were identified. In contrast to other Ocotea species, O. delicata has a wider diversity of kaempferol derivatives than quercetin. The biomass of the specimens showed a significant correlation with the chemical profile. The similarity among specimens was mostly determined by the concentrations of quinic acid, kaempferol glycosides, and boldine. The evaluated specimens exhibited chemical features similar to those of species classified as New World Ocotea, with the coexistence of aporphine and benzylisoquinoline alkaloids.
Insights into the Transcriptional Reprogramming of Peach Leaves Inoculated with Taphrina deformans
Authors: Maniatis Elissaios I. , Karamichali Ioanna , Stefanidou Eleni , Boutsika Anastasia , Tsitsigiannis Dimitrios I. , Paplomatas Epaminondas , Madesis Panagiotis , Zambounis Antonios
Published: March 2024

The dimorphic fungus Taphrina deformans is the causal agent of peach leaf curl disease, which affects leaves, flowers, and fruits. An RNA-seq approach was employed to gain insights into the transcriptional reprogramming of a peach cultivar during leaf inoculation with the yeast phase of the fungus across a compatible interaction. The results uncovered modulations of specific peach differentially expressed genes (DEGs) in peaches and pathways related to either the induction of host defense responses or pathogen colonization and disease spread. Expression profiles of DEGs were shown to be highly time-dependent and related to the presence of the two forms of the fungal growth, the inoculated yeast form and the later biotrophic phase during mycelial development. In parallel, this differential reprogramming was consistent with a diphasic detection of fungal load in the challenged leaves over the 120 h after inoculation (HAI) period. Leaf defense responses either occurred during the early yeast phase inoculation at 24 HAI, mediated primarily by cell wall modification processes, or more pronouncedly during the biotrophic phase at 72 HAI, as revealed by the activation of DEGs related to pathogen perception, signaling transduction, and secondary metabolism towards restraining further hypha proliferation. On the contrary, the expression patterns of specific DEGs at 120 HAI might further contribute to host susceptibility. These findings will further allow us to elucidate the molecular responses beyond the peach—T. deformans interaction.
Insights into Reactive Oxygen Species Production-Scavenging System Involved in Sugarcane Response to Xanthomonas albilineans Infection under Drought Stress
Authors: Wei Yao-Sheng , Zhao Jian-Ying , Javed Talha , Ali Ahmad , Huang Mei-Ting , Fu Hua-Ying , Zhang Hui-Li , Gao San-Ji
Published: March 2024

Plants must adapt to the complex effects of several stressors brought on by global warming, which may result in interaction and superposition effects between diverse stressors. Few reports are available on how drought stress affects Xanthomonas albilineans (Xa) infection in sugarcane (Saccharum spp. hybrids). Drought and leaf scald resistance were identified on 16 sugarcane cultivars using Xa inoculation and soil drought treatments, respectively. Subsequently, four cultivars contrasting to drought and leaf scald resistance were used to explore the mechanisms of drought affecting Xa–sugarcane interaction. Drought stress significantly increased the occurrence of leaf scald and Xa populations in susceptible cultivars but had no obvious effect on resistant cultivars. The ROS bursting and scavenging system was significantly activated in sugarcane in the process of Xa infection, particularly in the resistant cultivars. Compared with Xa infection alone, defense response via the ROS generating and scavenging system was obviously weakened in sugarcane (especially in susceptible cultivars) under Xa infection plus drought stress. Collectively, ROS might play a crucial role involving sugarcane defense against combined effects of Xa infection and drought stress.
Enhancing Native Plant Establishment in Mine Tailings under Drought Stress Conditions through the Application of Organo-Mineral Amendments and Microbial Inoculants
Authors: Atika Madline , Leila Benidire , Pereira Sofia I. A. , Castro Paula M. L. , Ali Boularbah
Published: March 2024

The implementation of phytoremediation strategies under arid and semiarid climates requires the use of appropriate plant species capable of withstanding multiple abiotic stresses. In this study, we assessed the combined effects of organo-mineral amendments and microbial inoculants on the chemical and biological properties of mine tailings, as well as on the growth of native plant species under drought stress conditions. Plants were cultivated in pots containing 1 kg of a mixture of mine tailings and topsoil (i.e., pre-mined superficial soil) in a 60:40 ratio, 6% marble sludge, and 10% sheep manure. Moreover, a consortium of four drought-resistant plant growth-promoting rhizobacteria (PGPR) was inoculated. Three irrigation levels were applied: well-watered, moderate water deficit, and severe water deficit, corresponding to 80%, 45%, and 30% of field capacity, respectively. The addition of topsoil and organo-mineral amendments to mine tailings significantly improved their chemical and biological properties, which were further enhanced by bacterial inoculation and plants’ establishment. Water stress negatively impacted enzymatic activities in amended tailings, resulting in a significant decrease in acid and alkaline phosphatases, urease, and dehydrogenase activities. Similar results were obtained for bacteria, fungi, and actinomycete abundance. PGPR inoculation positively influenced the availability of phosphorus, total nitrogen, and organic carbon, while it increased alkaline phosphatase, urease (by about 10%), and dehydrogenase activity (by 50%). The rhizosphere of Peganum harmala showed the highest enzymatic activity and number of culturable microorganisms, especially in inoculated treatments. Severe water deficit negatively affected plant growth, leading to a 40% reduction in the shoot biomass of both Atriplex halimus and Pennisetum setaceum compared to well-watered plants. P. harmala showed greater tolerance to water stress, evidenced by lower decreases observed in root and shoot length and dry weight compared to well-watered plants. The use of bioinoculants mitigated the negative effects of drought on P. harmala shoot biomass, resulting in an increase of up to 75% in the aerial biomass in plants exposed to severe water deficit. In conclusion, the results suggest that the combination of organo-mineral amendments, PGPR inoculation, and P. harmala represents a promising approach to enhance the phytoremediation of metal-polluted soils under semiarid conditions.
Correction: Blindow et al. Re-Establishment Techniques and Transplantations of Charophytes to Support Threatened Species. Plants 2021, 10, 1830
Authors: Blindow Irmgard , Carlsson Maria , van de Weyer Klaus
Published: March 2024

In the original publication [...]
Chemodiversity of Arctic Plant Dryas oxyodonta: LC-MS Profile and Antioxidant Activity
Authors: Kashchenko Nina I. , Olennikov Daniil N. , Chirikova Nadezhda K.
Published: March 2024

Dryas oxyodonta Yuz. is a perennial evergreen shrub from the Rosaceae family. D. oxyodonta thrives in subalpine and subarctic regions, as well as in highlands spanning from Central Asia to Siberia and Mongolia. Owing to a lack of information on its chemical composition, we conducted qualitative and quantitative chromatographic analyses on extracts from the leaves and flowers of D. oxyodonta sourced from various Siberian habitats. Employing high-performance liquid chromatography with photodiode-array detection and electrospray ionization triple-quadrupole mass spectrometric detection, we identified 40 compounds, encompassing gallotannins, hydroxycinnamates, procyanidins, catechins, flavonoids, and triterpenes. All Siberian populations of D. oxyodonta exhibited a notable abundance of phenolic compounds. Furthermore, we identified rare glycosides, such as sexangularetin and corniculatusin, as potential markers of the chemodiversity within the Dryas genus. Extracts from the flowers and leaves were effective scavengers of free radicals, including DPPH•, ABTS•+−, O2•−, and •OH radicals. Our findings unequivocally establish D. oxyodonta as a rich source of phenolic compounds with potent antioxidant activity, suggesting its potential utility in developing novel functional products.
Point Source Capture of Methane Using Ionic Liquids in Packed Bed Absorbers/Strippers: Experimental and Modelling
Authors: Rahimpour Hamid Reza , Zanganeh Jafar , Moghtaderi Behdad
Published: March 2024

Fugitive methane emissions from the mining industry, particularly so-called ventilation air methane (VAM) emissions, are considered among the largest sources of greenhouse gas (GHG) emissions. VAM emissions not only contribute to the global warming but also pose a significant hazard to mining safety due to the risk of accidental fires and explosions. This research presents a novel approach that investigates the capture of CH4 in a controlled environment using 1-butyl-3-methylimidazolium bis (trifluoromethylsulfonyl) imide [BMIM][TF2N] ionic liquid (IL), which is an environmentally friendly solvent. The experimental and modelling results confirm that CH4 absorption in [BMIM][TF2N], in a packed column, can be a promising technique for capturing CH4 from point sources, particularly the outlet streams of ventilation shafts in underground coal mines, which typically accounts for <1% v/v of the flow. This study assessed the effectiveness of CH4 removal in a packed bed column by testing various factors such as absorption temperature, liquid and gas flow rates, flow pattern, packing size, desorption temperature, and desorption pressure. According to the optimisation results, the following parameters can be used to achieve a CH4 removal efficiency of 23.8%: a gas flow rate of 0.1 L/min, a liquid flow rate of 0.5 L/min, a packing diameter of 6 mm, and absorption and desorption temperatures of 303 K and 403.15 K, respectively. Additionally, the experimental results indicated that ILs could concentrate CH4 in the simulated VAM stream by approximately 4 fold. It is important to note that the efficiency of CH4 removal was determined to be 3.5-fold higher compared to that of N2. Consequently, even though the VAM stream primarily contains N2, the IL used in the same stream shows a notably superior capacity for removing CH4 compared to N2. Furthermore, CH4 absorption with [BMIM][TF2N] is based on physical interactions, leading to reduced energy requirements for regeneration. These findings validate the method’s effectiveness in mitigating CH4 emissions within the mining sector and enabling the concentration of VAM through a secure and energy-efficient procedure.
Fluent Integration of Laboratory Data into Biocatalytic Process Simulation Using EnzymeML, DWSIM, and Ontologies
Authors: Behr Alexander S. , Surkamp Julia , Abbaspour Elnaz , Häußler Max , Lütz Stephan , Pleiss Jürgen , Kockmann Norbert , Rosenthal Katrin
Published: March 2024

The importance of biocatalysis for ecologically sustainable syntheses in the chemical industry and for applications in everyday life is increasing. To design efficient applications, it is important to know the related enzyme kinetics; however, the measurement is laborious and error-prone. Flow reactors are suitable for rapid reaction parameter screening; here, a novel workflow is proposed including digital image processing (DIP) for the quantification of product concentrations, and the use of structured data acquisition with EnzymeML spreadsheets combined with ontology-based semantic information, leading to rapid and smooth data integration into a simulation tool for kinetics evaluation. One of the major findings is that a flexibly adaptive ontology is essential for FAIR (findability, accessibility, interoperability, reusability) data handling. Further, Python interfaces enable consistent data transfer.
Research on an Optimal Maintenance and Inventory Model Based on Carbon Tax Policy
Authors: Chen Wei-Jen , Lu Chi-Jie , Hsu Pei-Ti , Yang Chih-Te
Published: March 2024

The equipment in a factory will gradually deteriorate during production, leading to the production of defective products. Without appropriate maintenance, the defect rate will increase over time. Consequently, the production cost will rise, the inventory quality will be affected, the profit will decrease, and the risk of carbon emissions will increase, leading to more customer complaints and damaging the corporate image. In addition to focusing on preventive maintenance to ensure the quality of products, companies should also take carbon emissions into consideration. Furthermore, the frequency of maintenance must be carefully considered, as both carbon emissions and maintenance costs will increase if the frequency is too high; conversely, if the maintenance frequency is too low or non-existent, the defect rate may increase cumulatively, or production may be suspended due to equipment failure. Therefore, this research explores preventive maintenance and inventory management issues within an imperfect production system and develops an extended economic production quantity model that incorporates defective products as well as taking carbon tax and preventive maintenance into consideration. The main purpose is to determine the optimal maintenance frequency, production, and replenishment cycle length, so as to maximize the total profit under the carbon tax policy. This study demonstrates a computing process with relatively impractical product data based on the actual business situation of a disposable diaper manufacturer. Furthermore, a sensitivity analysis is implemented to the model parameters in the proposed model. The managemental insights are illustrated based on the results of theoretical analysis to provide a reference to policy makers during decision making, hence, to secure the sustainability and green transitions of corporates. The results of this study not only help to reduce environmental impact but can also improve the competitiveness and sustainable development of enterprises.
Reconstructing the First “Iron Hand” of Knight Götz von Berlichingen and Its Derived Modern Developments: Back to the Future
Authors: Otte Andreas , Hazubski Simon
Published: March 2024

“Ad fontes!” [...]
A Robust Indicator Mean-Based Method for Estimating Generalizability Theory Absolute Error and Related Dependability Indices within Structural Equation Modeling Frameworks
Authors: Lee Hyeryung , Vispoel Walter P.
Published: March 2024

In this study, we introduce a novel and robust approach for computing Generalizability Theory (GT) absolute error and related dependability indices using indicator intercepts that represent observed means within structural equation models (SEMs). We demonstrate the applicability of our method using one-, two-, and three-facet designs with self-report measures having varying numbers of scale points. Results for the indicator mean-based method align well with those obtained from the GENOVA and R gtheory packages for doing conventional GT analyses and improve upon previously suggested methods for deriving absolute error and corresponding dependability indices from SEMs when analyzing three-facet designs. We further extend our approach to derive Monte Carlo confidence intervals for all key indices and to incorporate estimation procedures that correct for scale coarseness effects commonly observed when analyzing binary or ordinal data.
Teaching and Learning Principles in the Church of Jesus Christ of Latter-Day Saints: A Thematic Analysis of the Doctrine and Covenants
Authors: Calvert Isaac , Bengtzen Sabrina , Ashcraft Jessica
Published: March 2024

This article presents pedagogic principles prescribed in the text of the Doctrine and Covenants, a foundational book of scripture from The Church of Jesus Christ of Latter-day Saints. Following a qualitative, thematic analysis of the text, we organized the data into seven themes: character of God as a teacher, learning as the pursuit of truth, attributes of a teacher and learner, warnings in seeking light and truth, learning methods, teaching methods, and non-compulsory teaching and learning. Most noteworthy among these themes is the text’s description of learning as the pursuit of embodied ontological truth and the fundamental questions it raises about the nature of compulsion in learning.