bluuebunny/crossref_metadata_2025 huggingface dataset:
Modeling oxygenation in venous blood and skeletal muscle in response to exercise using near-infrared spectroscopy
Authors: Lai Nicola , Zhou Haiying , Saidel Gerald M. , Wolf Martin , McCully Kevin , Gladden L. Bruce , Cabrera Marco E.
Published: April 2009

Noninvasive, continuous measurements in vivo are commonly used to make inferences about mechanisms controlling internal and external respiration during exercise. In particular, the dynamic response of muscle oxygenation ([Formula: see text]) measured by near-infrared spectroscopy (NIRS) is assumed to be correlated to that of venous oxygen saturation (SvO2) measured invasively. However, there are situations where the dynamics of [Formula: see text] and SvO2 do not follow the same pattern. A quantitative analysis of venous and muscle oxygenation dynamics during exercise is necessary to explain the links between different patterns observed experimentally. For this purpose, a mathematical model of oxygen transport and utilization that accounts for the relative contribution of hemoglobin (Hb) and myoglobin (Mb) to the NIRS signal was developed. This model includes changes in microvascular composition within skeletal muscle during exercise and integrates experimental data in a consistent and mechanistic manner. Three subjects (age 25.6 ± 0.6 yr) performed square-wave moderate exercise on a cycle ergometer under normoxic and hypoxic conditions while muscle oxygenation (Coxy) and deoxygenation (Cdeoxy) were measured by NIRS. Under normoxia, the oxygenated Hb/Mb concentration (Coxy) drops rapidly at the onset of exercise and then increases monotonically. Under hypoxia, Coxy decreases exponentially to a steady state within ∼2 min. In contrast, model simulations of venous oxygen concentration show an exponential decrease under both conditions due to the imbalance between oxygen delivery and consumption at the onset of exercise. Also, model simulations that distinguish the dynamic responses of oxy-and deoxygenated Hb (HbO2, HHb) and Mb (MbO2, HMb) concentrations (Coxy = HbO2 + MbO2; Cdeoxy = HHb + HMb) show that Hb and Mb contributions to the NIRS signal are comparable. Analysis of NIRS signal components during exercise with a mechanistic model of oxygen transport and metabolism indicates that changes in oxygenated Hb and Mb are responsible for different patterns of [Formula: see text] and SvO2 dynamics observed under normoxia and hypoxia.
The impact of exercise on derived measures of central pressure and augmentation index obtained from the SphygmoCor device
Authors: Dawson Ellen A. , Black Mark A. , Pybis Jennifer , Cable N. Timothy , Green Daniel J.
Published: April 2009

The purpose of this study was to investigate whether measures derived from the SphygmoCor device and its associated transfer function are influenced by exercise-induced alterations in vascular tone. Measurements were taken from either the exercised or the contralateral nonexercised limb during repeated and identical incremental hand-grip protocols. Eight male subjects performed three 3-min bouts of hand-grip exercise on two occasions. The exercise intensities were set at 3 kg, 5 kg, with a final 1.5-kg bout performed during cuff ischemia (1.5Isch). Blood pressure waveforms were recorded from the radial artery of either the exercised or nonexercised limb using applanation tonometry (SphygmoCor) during a 90-s rest period immediately after each exercise bout. Central blood pressures and augmentation indexes (AIx), an index of arterial stiffness, were derived using the peripheral waveform and the inbuilt SphygmoCor transfer function (TF). AIx was consistently ∼10% higher in the exercised arm during all trials compared with the nonexercised limb. Similarly, there was a consistent and significant difference (∼3 mmHg; P < 0.05) between exercised and nonexercised arms for the derived central systolic and mean arterial blood pressures. Despite identical bouts of exercise, AIx and central systolic and mean arterial blood pressures derived from applanation tonometry at the peripheral radial artery were statistically different when assessed at the exercising arm vs. the nonexercising arm. Changes in vascular tone with exercise may modify the intrinsic characteristics of the vessel wall and could compromise the assumptions underlying transfer functions used to derive central measures using applanation tonometry.
Spousal Interrelations in Old Age – A Mini-Review
Authors: Hoppmann Christiane , Gerstorf Denis
Published: April 2009

We review findings on spousal interrelations in old age in such key domains as cognition, well-being, and health. Therein, we demonstrate that spousal interrelations may extend developmental options but may also make an individual vulnerable to the experience of loss. We address theoretical questions concerning possible underlying mechanisms, e.g. individual and spousal goal-related processes. Furthermore, we draw attention to important methodological challenges such as identifying processes that operate along different time scales and employing adequate data analytic tools. We propose that aging research may benefit from an examination of interrelations in developmental pathways of multiple co-developing individuals such as spouses and point to the need to disentangle individual from relationship-specific effects.
Feasibility of the Radiofrequency Hot Balloon Catheter for Isolation of the Posterior Left Atrium and Pulmonary Veins for the Treatment of Atrial Fibrillation
Authors: Sohara Hiroshi , Takeda Hiroshi , Ueno Hideki , Oda Toshiyuki , Satake Shutaro
Published: April 2009

Background—

Atrial fibrillation originates mostly from the pulmonary vein (PV) foci or non-PV foci in the posterior left atrium (LA). The present study was designed to evaluate the feasibility and safety of a novel radiofrequency hot balloon catheter for the treatment of patients with atrial fibrillation by electrically isolating the posterior LA, including all PVs.

Methods and Results—

One hundred consecutive patients with drug-resistant atrial fibrillation (63 paroxysmal, 37 persistent) were enrolled. The isolation of the PVs was performed by wedging the balloon at each PV antrum to create circumferential lesions in each case. Contiguous linear lesions were also created at the roof between the superior PVs and at the bottom of the posterior LA between the inferior PVs by dragging the balloon along the endocardium. Complete elimination of the posterior LA and PV potentials was achieved in all 100 cases, confirmed by either conventional or electro-anatomic mapping system. The total procedure time was 129�26 minutes, inclusive of 29.9�7.3 minutes of fluoroscopy time. Follow-up during 11.0�4.8 months confirmed that 92 patients (60 paroxysmal, 32 persistent) were free from atrial fibrillation without antiarrhythmic drugs, and in the remaining patients except for 2 with LA tachycardia, sinus rhythm was maintained with antiarrhythmic drugs. With precautions of esophageal cooling by irrigation dictated by temperature monitoring and monitoring phrenic nerve pacing, no LA-esophageal fistula or permanent phrenic nerve injury occurred.

Conclusion—

This feasibility study supports the safety and efficacy of radiofrequency hot balloon catheter for complete isolation of the posterior LA and PVs.
Adiponectin Suppresses Pathological Microvessel Formation in Retina Through Modulation of Tumor Necrosis Factor-α Expression
Authors: Higuchi Akiko , Ohashi Koji , Kihara Shinji , Walsh Kenneth , Ouchi Noriyuki
Published: April 2009

The fat-derived hormone adiponectin has been shown to have a protective role in macrovascular disorders. However, nothing is known about the function of adiponectin in retinal microvessel disease. Here, we investigated the causal role of adiponectin in retinal vessel formation and inflammation under conditions of hypoxia. When neonatal mice were subjected to ischemia-induced retinopathy, pathological retinal neovascularization during ischemia was exacerbated in adiponectin-knockout (APN-KO) mice compared with wild-type mice (neovascular area: 17.0±1.0% versus 11.7±0.6%, respectively). APN-KO mice also exhibited increased leukocyte adhesion (2.3±0.4-fold) and tumor necrosis factor (TNF)-α expression (2.6±0.2-fold) in hypoxic retina. Adenovirus-mediated overexpression of adiponectin attenuated hypoxia-induced pathological retinal neovascularization by 35% in wild-type mice and by 40% in APN-KO mice and leukostasis by 64% in wild-type mice and by 75% in APN-KO mice, which were associated with reduced TNF-α production. TNF-α blockade diminished the enhanced pathological neovascularization in APN-KO mice by 34%, and the inhibitory effects of adiponectin overexpression on retinal neovascularization and leukocyte adhesion were abolished in mice lacking TNF-α. These data provide evidence that adiponectin protects against retinal vessel injury following pathological stimuli through modulation of TNF-α inflammatory responses.
The Analyst's Reluctance To Begin a New Analysis
Authors: Theodorou Ehrlich Lena
Published: April 2009

Given the decline in the average psychoanalytic practice, it is crucial to examine the variables affecting the individual analyst's practice. One such variable is the analyst's reluctance to begin a new analysis. Literature exploring its origins, possible manifestations, and effects on the analyst's thinking and practicing is reviewed. The analyst's reluctance is considered (1) as a defense against powerful affects, (2) as a co-created resistance, and (3) as a manifestation of the analyst's conflicts. Two clinical examples illustrate how this reluctance and its subsequent recognition influence the analyst's work. It is suggested that the present reality of a socioeconomic climate adverse to psychoanalysis, with fewer patients willing to engage in analysis from the outset, might be used to rationalize the analyst's reluctance to begin. It is also suggested that the analyst's reluctance to begin a new analysis is much more pervasive and influential than is presently recognized.
The Role of an Early-Life Variant of the Oedipus Complex in Motivating Religious Endeavors
Authors: Osman Marvin P.
Published: April 2009

A close reading of sources Freud used in writing Totem and Taboo supports the thesis that a predecessor archaic oedipus complex is instrumental in motivating religious worship. This early-life complex manifests a psychodynamic in which birth, growth, and self-realization, to varying degree in each individual, tend to be psychically correlated with diminution and harm vis-à-vis one's procreators. As a result, the psychodynamic is likely to induce unease over youth exercising its powers. The story of Adam and Eve, which depicts a growing self-determination being stymied and coming to grief, is a mythic epitome of this psychodynamic. Religious practices serve to expiate the sense of unease, partly by replenishing and even recasting seemingly diminished procreators, through myth and ritual, into omnipotent, immortal entities, and partly by reversing individuation's challenge to authority by exhorting submission to, and even union, with the divine parent. The sources used in demonstrating the various means whereby religious practices serve to ameliorate the burden of “original sin” include W. R. Smith (1894), the Old Testament, and studies of archaic religious rites, including those of the Aztecs.
Analytic Preparation: the Creation of an Analytic Climate With Patients Not Yet in Analysis
Authors: Wilson Arnold
Published: April 2009

The art and science of beginning an analysis has a life of its own and can be considered in many ways quite apart from its later stages. An incremental path forward is smoothed for patients who are not yet prepared to analyze, and builds eventually into something readily recognizable as an analysis. The term analytic preparation refers to this set of processes. Early on, the analyst is concerned less with facilitating an early replica of an idealized analysis than with facilitating the mutual adaptation of patient and analyst as they begin to negotiate a “thought community.” Since analytic preparation is not an entity, it does not neatly overlap in real time with the opening phase as usually described, does not have a discrete beginning or end, and does not abruptly shift midstream into analysis proper. Some relations between analytic preparation, analytic interaction, and the interpretation of transference are examined.
Writing About Patients: V. Analysts Reading About Themselves as Patients
Authors: Kantrowitz Judy L.
Published: April 2009

The narratives of twenty analysts who were written about when they were patients are presented. Their narratives provide suggestions about practices to avoid when writing clinical material, but no generalized prescriptions emerge. Individualization and sensitivity to the situation for each patient-analyst pair remain the best guide. The experiences these analysts recount run the gamut of emotions, from negative through neutral to positive. The neutral responses came mainly from twelve analysts who in the course of an interview about their own writing told of having been written about as patients. The other eight, volunteers who initiated contact for the sole purpose of reporting their experience of having been written about, appear on average to be motivated by stronger affective reactions. The era in which the analyst wrote also seems to have influenced the reactions; in earlier times, not asking permission was accepted professional practice. Today, however, it is increasingly common to ask permission when extended clinical examples are published. One problem specific to analyst-patients was concern about the loss of their role as patient when their analyst engaged them collaboratively in the writing.
Child Psychoanalysis and Obsessive-Compulsive Symptoms: The Treatment of a Ten-Year-Old Boy
Authors: McGehee Rex H.
Published: April 2009

Obsessive-compulsive symptoms have long held interest for psychoanalysts. Currently, pharmacological and behavioral treatments are commonly viewed as the standard of care, and psychoanalytic clinicians are often uncertain of how best to approach these patients. The successful two-year psychoanalytic treatment of a ten-year-old boy with obsessive-compulsive disorder, who remained symptom-free eight years after termination, is reported. While this is but a single case, its outcome implies that for some patients psychoanalysis may be the treatment of choice.
Intuition: A Bridge To the Coenesthetic World of Experience
Authors: Piha Heikki
Published: April 2009

The concept of intuition is relatively unestablished in psychoanalysis, where it is often associated with narcissistic meanings and vagueness. But intuition, as an integrated mode of archaic coenesthetic thinking, should be kept conceptually free of those connotations. Its capacity of undifferentiated delineation supplies an instinctive general means of dealing immediately with various rationally indistinct phenomena, such as forms, shades, and multidimensionality, regardless of the boundaries between sensory modalities. It may be impossible to translate intuitive experiences into lexical form; these languages are incommensurable. Intuition as a preconscious nondiscursive thinking process is needed in creativity, as well as less conspicuously in countless everyday activities. In speech communication, intuition rapidly specifies subtle shades of meaning in linguistic content and all the prosody. In psychoanalytic work intuition is like radar, creating preliminary contacts with the inner world of the analysand. The observations gained require, however, rational consideration to be confirmed. Intuition is an essential instrument of the psychoanalyst, and also functions in the service of tact to create working space and adequate forms of interpretations. Clinical vignettes reflecting some problematic fates of special intuitiveness in creativity are presented from psychoanalytic work with artists.
On Analytic Process
Authors: Jones Enrico E. , Ablon J. Stuart
Published: April 2009

An innovative methodology is presented for identifying and assessing change process in psychoanalytic treatments. Using the Psychotherapy Process Q-set (PQS), a panel of experienced psychoanalysts developed a prototype of an ideal psychoanalytic hour. This prototype was then applied to verbatim transcripts of three archived treatment samples: psychoanalyses, long-term analytic therapies, and brief psychodynamic therapies. The degree to which these treatments fostered an analytic process as represented by the prototype was measured quantitatively. Analytic process was significantly more present in psychoanalyses than in the long-term analytic therapies, which, in turn fostered significantly more analytic process than did brief psychodynamic therapies. The study demonstrates that, given descriptive language that does not represent a particular theoretical perspective, analysts can agree on a definition of analytic process, and that analytic process can be operationalized and quantitatively assessed. A second study demonstrates that despite consensus on its definition, there is not just one proper analytic process; rather, there are change processes unique to each dyad. Two quantitative case studies illustrate how each analytic pair has a unique interaction pattern linked to treatment progress. These dyad-unique “interaction structures” are recurrent, mutually influencing patterns of interaction, the experience, recognition, and comprehension of which appear to be a fundamental component of therapeutic action. A bipersonal model is described that attempts to bridge theories of therapeutic action that focus on insight and self-understanding and those that emphasize the patient's experience of the therapist.
Empirical Evidence Supporting the Conceptual Relatedness of Object Representations and Internal Working Models
Authors: Goodman Geoff
Published: April 2009

The proliferation of new developmental and clinical theories broadly termed “psychoanalytic” demands a methodology for making systematic comparisons to establish the commonalities and distinctions among them. This article presents an empirical method for the comparative evaluation of such theories. The Mother-To-Child Object Representation / Internal Working Model Q-sort is a 100-item instrument constructed to assess the quality of the prototypical mother's object representation and internal working model of her child at age five. Object relations judges were asked to sort these items for a complex, differentiated, integrated object representation of a five-year-old, attachment judges for a secure, coherent, freely valuing internal working model of a five-year-old. Judges' criterion Q-sorts in each group yielded a composite criterion Q-sort of the quality of the prototypical mother's mental representation of her child according to each group's theoretical perspective. The Spearman-Brown correlation between the two composite criterion Q-sorts was r = .90, p < .001, suggesting that when confined to a 100-item common vocabulary, judges representing each theoretical construct agreed on the conceptual relatedness of object representations and internal working models. The theoretical constructs of object representations and internal working models share common assumptions in need of further exploration.
The Capacity To Forgive: Intrapsychic and Developmental Perspectives
Authors: Horwitz Leonard
Published: April 2009

The topic of forgiveness, despite its importance for the continuity of relationships and for the mental health of the aggrieved party, is relatively neglected in the psychoanalytic literature, perhaps because it is often seen as the province of religion and carries the connotation of reaction formation and inauthenticity. However, genuine forgiveness involves significant intrapsychic work, conscious and unconscious working through of one's anger, and putting the offense into the context of an integrated view of the whole person of the offender. Early developmental structures are the ground on which the relative ability to let go of a grievance depends. While later motives and defenses (e.g., fear of retraumatization, avoidance of shame) may also play a role, these early structures are primary. They are described here in terms of attainment of the depressive position and the development of a sense of secure attachment, the capacity to mentalize, and the ability to mourn.
Exploring Patterns of Court-Ordered Mental Health Services for Juvenile Offenders
Authors: Dannerbeck Janku Anne , Jiahui Yan
Published: April 2009

Objective assessments of the risks and needs of court-involved youth may lead to judicial processing that is less vulnerable to actual or perceived racial discrimination. Using data from a statewide assessment system on African American and Caucasian status offenders and delinquents formally processed in one Missouri circuit over a 1-year period, the authors examined the association of race and other factors on court orders for mental health—related services. Relative to their population distribution, African Americans were overrepresented in the juvenile system and underrepresented in orders for mental health services. In the logit analysis, negative attitudes and behavior problems carried strong associations with orders for mental health services. Risk and need, not bias, were mostly strongly associated with orders for mental health treatment.
Matching Court-Ordered Services with Treatment Needs
Authors: Vieira Tracey A. , Skilling Tracey A. , Peterson-Badali Michele
Published: April 2009

The rehabilitation of young offenders and their reintegration into society are important goals of the juvenile justice system. An empirically supported model of service delivery attending to the principles of risk level, criminogenic need, and responsivity provides direction in achieving these goals. Although research on this model thus far has evaluated the principles only at a group level, the present study evaluates the impact on recidivism of matching youth with services at the individual level. Files of 122 youth who received court assessments were reviewed to determine whether clinical recommendations and services received were congruent. Youths' criminal records were reviewed to determine reoffense outcomes. As predicted, higher risk scores were associated with earlier and more frequent recidivism. Knowing whether a young offender had his or her specific criminogenic needs addressed in treatment added to the predictive power of risk. Having only a few treatment needs met was associated with significantly earlier recidivism and a greater number of new convictions. These findings may provide direction in enhancing efforts to effectively respond to youth crime.
Outcome Trajectories in Drug Court
Authors: DeMatteo David , Marlowe Douglas B. , Festinger David S. , Arabia Patricia L.
Published: April 2009

Graduation rates in drug courts average 50% to 70%, but it is unclear what proportion of graduates responded to the drug court services and what proportion might not have had serious drug problems on entry. This study cluster analyzes urine drug screen results during the first 14 weeks of treatment on 284 participants from three misdemeanor drug courts. A four-cluster solution ( R2 > .75) produced distinct subgroups characterized by (a) consistently drug-negative urine specimens (34% of the sample), (b) consistently drug-positive specimens (21%), (c) consistently missed urine specimens (26%), and (d) urine specimens that began as drug positive but became progressively drug negative over time (19%). These data suggest that approximately one third of the participants might not have had serious drug problems on entry. Approximately one fifth appeared to respond to drug court services, and nearly one half continued to exhibit problems after 14 weeks. Implications for adaptive programming in drug courts are discussed.
The Rise of Mega-Church Efforts in International Development
Authors: Gramby-Sobukwe Sharon , Hoiland Tim
Published: April 2009

Evangelical `mega-churches' are awakening to the importance of tackling poverty and social issues even as they continue their focus on evangelism and church planting. As a result, they are moving into carrying out their own development efforts globally, emphasizing direct relationships with churches in the developing world. This is changing their engagement with the `professional' evangelical development organizations, leading the mega-churches to search and experiment with development methods, approaches, and choice of issues, and bringing them up against questions and challenges inherent in development work.
Caring for Creation and Culture
Authors: LeQuire Stan L. , Van Gilder Katie
Published: April 2009

There is a growing phenomenon of evangelical NGOs engaging in ecotourism businesses. Preliminary research and case studies show that there is a diversity of approaches and methods among them, including seeing ecotourism as a method to alleviate local poverty, care for the environment and stand in solidarity with indigenous cultures. Based on this research, a theological framework is proposed to help organizations engaged in community-based ecotourism think through their endeavors in this field.
Emboli occurrence during coronary artery bypass surgery: the influence of a new method of perfusionist blood sampling
Authors: Sauren LD , Mooren EJ , Severdija EE , Weerwind PW , Maessen JG
Published: April 2009

Peri-operative cerebral microemboli in cardiac surgery are associated with post-operative neurological complications. Cardiopulmonary bypass (CPB) and perfusionist interventions are important contributors of microemboli. In this study, we examined the influence of blood sampling by the perfusionist on the appearance of microembolic signals (MES) in an open and a closed CPB system. Fourteen patients underwent isolated coronary artery bypass grafting (CABG), using either an open or closed CPB system. Patients were monitored with transcranial Doppler (TCD) for the occurrence MES in both middle cerebral arteries (MCA) as well as in the arterial and venous lines of the CPB. In the closed system, two sampling methods were used, namely the “traditional” and the “E-line”. In the latter, a shunt line was applied from the manifold to the cardiotomy reservoir). In the open system, one method of blood sampling was used. Blood sampling in an open system or in a closed system using the E-line resulted in 0.2 (±0.56) MES, which was significantly lower than the traditional method (72 (±69) MES). The use of a shunt line reduces MES during blood sampling in a closed CPB system.
Effect of clenbuterol administration on the healthy murine heart
Authors: Moore D , Anderson M , Larson DF
Published: April 2009

Clenbuterol has recently been shown to reverse pathologic cardiac remodeling during left ventricular assist device (LVAD) support, leading to restored ventricular function and explantation of LVAD devices. However, others have not been able to support these observations. Our hypothesis is that the β2-adrenergic activity of clenbuterol induces cardiac extracellular matrix (ECM) remodeling, resulting in increased interstitial fibrillar collagen content and altered diastolic function that may account for these conflicting reports. The intent of this study is to characterize the effect of clenbuterol on healthy murine hearts with transthoracic echo and histology. C57BL/6 female mice were administered 2.4 µg/kg/day of clenbuterol in the drinking water for 7 days and analysis conducted on day 8–24 hours after the last dose of clenbuterol. Histological analysis demonstrated an increase in left ventricular ECM collagen content in a control group compared with the clenbuterol group (density 0.32 ± 0.16 compared to 2.01 ± 0.30 RD/mm2). The ventricular fibrosis was supported by altered diastolic function measured by transthoracic echo where there was a significant increase in isovolumic relaxation time, and left atrial dimension and a decrease in left ventricular free wall tissue Doppler ratios. Our study showed no significant differences in left ventricular ejection fraction, cardiac output, or heart rate between the clenbuterol and control groups. These data suggest that the β-2 adrenergic activity of clenbuterol increases ECM fibrillar collagen concentrations in normal hearts, resulting in altered diastolic function.
Circadian Regulation of Olfactory Receptor Neurons in the Cockroach Antenna
Authors: Saifullah A.S.M. , Page Terry L.
Published: April 2009

In the cockroach, olfactory sensitivity as measured by the amplitude of the electroantennogram (EAG) is regulated by the circadian system. We wished to determine how this rhythm in antennal response was reflected in the activity of individual olfactory receptor neurons. The amplitude of the EAG and the activity of olfactory receptor neurons (ORNs) in single olfactory sensilla were recorded simultaneously for 3 to 5 days in constant darkness from an antenna of the cockroach Leucophaea maderae. Both EAG amplitude and the spike frequency of the ORNs exhibited circadian rhythms with peak amplitude/activity occurring in the subjective day. The phases of the rhythms were dependent on the phase of the prior light cycle and thus were entrainable by light. Ablation of the optic lobes abolished the rhythm in EAG amplitude as has been previously reported. In contrast, the rhythm in ORN response persisted following surgery. These results indicated that a circadian clock outside the optic lobes can regulate the responses of olfactory receptor neurons and further that this modulation of the ORN response is not dependent on the circadian rhythm in EAG amplitude.
Vasoactive Intestinal Polypeptide Entrains Circadian Rhythms in Astrocytes
Authors: Marpegan Luciano , Krall Thomas J. , Herzog Erik D.
Published: April 2009

Many mammalian cell types show daily rhythms in gene expression driven by a circadian pacemaker. For example, cultured astrocytes display circadian rhythms in Period1 and Period2 expression. It is not known, however, how or which intercellular factors synchronize and sustain rhythmicity in astrocytes. Because astrocytes are highly sensitive to vasoactive intestinal polypeptide (VIP), a neuropeptide released by neurons and important for the coordination of daily cycling, the authors hypothesized that VIP entrains circadian rhythms in astrocytes. They used astrocyte cultures derived from knock-in mice containing a bioluminescent reporter of PERIOD2 (PER2) protein, to assess the effects of VIP on the rhythmic properties of astrocytes. VIP induced a dose-dependent increase in the peak-to-trough amplitude of the ensemble rhythms of PER2 expression with maximal effects near 100 nM VIP and threshold values between 0.1 and 1 nM. VIP also induced dose- and phase-dependent shifts in PER2 rhythms and daily VIP administration entrained bioluminescence rhythms of astrocytes to a predicted phase angle. This is the first demonstration that a neuropeptide can entrain glial cells to a phase predicted by a phase-response curve. The authors conclude that VIP potently entrains astrocytes in vitro and is a candidate for coordinating daily rhythms among glia in the brain.
The Cyclic GMP Modulators YC-1 and Zaprinast Reduce Vessel Remodeling Through Antiproliferative and Proapoptotic Effects
Authors: Keswani Amit N. , Peyton Kelly J. , Durante William , Schafer Andrew I. , Tulis David A.
Published: April 2009

Guanosine-specific cyclic nucleotide signaling is suggested to serve protective actions in the vasculature; however, the influence of selective pharmacologic modulation of cyclic guanosine monophosphate— synthesizing soluble guanylate cyclase or cyclic guanosine monophosphate—degrading phosphodiesterase on vessel remodeling has not been thoroughly examined. In this study, rat carotid artery balloon injury was performed and the growth-modulating effects of the soluble guanylate cyclase stimulator YC-1 or the cyclic guanosine monophosphate—dependent phosphodiesterase-V inhibitor zaprinast were examined. YC-1 or zaprinast elevated vessel cyclic guanosine monophosphate content, reduced medial wall and neointimal cell proliferation, stimulated medial and neointimal cellular apoptosis, and markedly attenuated neointimal remodeling in comparable fashion. Interestingly, soluble guanylate cyclase inhibition by 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one failed to noticeably alter neointimal growth, and concomitant zaprinast with YC-1 did not modify any parameter compared to individual treatments. These results provide novel in vivo evidence that YC-1 and zaprinast inhibit injury-induced vascular remodeling through antimitogenic and proapoptotic actions and may offer promising therapeutic approaches against vasoproliferative disorders.
Implementing Mental Health Treatment for Batterer Program Participants
Authors: Gondolf Edward W.
Published: April 2009

The implementation of a screening and referral system for supplemental mental health treatment among batterer program participants was investigated in a 2-year formative evaluation. The research team conducted direct observation of the agency procedures, participation in training and supervision meetings, debriefing interviews with administrators, and informal conversations with staff and clients. Inconsistencies and breakdowns associated with nearly every step of the screening and referral process were identified, for example, notification of referral instructions and verification of clinical compliance. Court sanctions for referral noncompliance remained inconsistent even during court-mandated referral. Several underlying issues were also exposed: administrative absenteeism and turnover, administrative-staff gaps, client overload, and differing agency priorities. These issues reinforce the challenges facing coordinated community response.
Do Business Communication Technology Tools Meet Learner Needs?
Authors: DuFrene Debbie D. , Lehman Carol M. , Kellermanns Franz W. , Pearson Rodney A.
Published: April 2009

While institutions of higher education are enthusiastically embracing technology-mediated learning (TML), little research has been conducted to identify factors that influence student use of TML tools or determine whether use of them increases student learning. This study of business communication students at two universities found that (1) students tend to be sensing, visual, active, and sequential learners; (2) perceived usefulness and perceived ease of use of TML tools are positively associated with perceived learning success; (3) learning styles do influence the students' usage behavior of certain TML tools; and (4) students' sensing/intuitive learning style is related to their perceived learning success.
Using Social Stories and Visual Schedules to Improve Socially Appropriate Behaviors in Children With Autism
Authors: Schneider Naomi , Goldstein Howard
Published: April 2009

The current study investigated the effects of Social Stories written according to Gray’s specifications on on-task behavior in inclusive classroom settings in three children with autism. Using a multiple-baseline design across participants, modest improvements in on-task behavior were associated with implementation of an auditory-visual Social Story intervention. In follow-up analysis, the Social Story was replaced with a visual schedule component to augment the effects of Social Stories when there was room for improvement for one participant. Further improvement in on-task behavior indicates that strategies such as visual schedules may be an effective way to augment the effects of Social Stories. An effect size estimate calculated using Parker et al.’s percentage of all nonoverlapping data points procedure revealed a large effect ( d = 1.33) associated with Social Stories alone, which increased ( d = 1.7) when the visual schedule intervention applied to one participant was added to the analysis. Although Social Stories produced improvements in on-task behavior in children with autism, additional components, such as visual schedules, may be useful for optimizing performance.
The subjects of criminal identification
Authors: Pavlich George
Published: April 2009

This article analyses two late 19th-century technologies that sought to identify criminals with scientific accuracy: Alphonse Bertillon's techniques for measuring bodies and Francis Galton's composite portraits of criminal types. It analyzes the regulatory environment in England in which their ideas achieved considerable prominence, emphasizing crucial differences in the visions of `science' embraced by the two men. By highlighting their different claims to science, this article outlines their respective legacies in criminal identification arenas, and isolates unique dangers associated with each. Those dangers variously alert us to the importance of questioning a persistent, if often implicit, sentiment within much criminal justice thinking; namely, by identifying individual criminals, or criminal types, justice systems effectively address the problem of crime. Against this approach, and resonant with a critical criminology in search of less exclusionary ways to govern, the following analysis considers criminal identification not as a discovery but a creation. In so doing, it seeks to re-politicize current practices of criminalization, challenge claims to the purported scientific impartiality of criminal identification and embrace the possibility of justice beyond a dominant reflex to create criminals.
Rawls on the practicability of utilitarianism
Authors: Labukt Ivar
Published: April 2009

John Rawls's claim to have demonstrated the superiority of his own two principles of justice to the principle of utility has generated fairly extensive critical discussion. However, this discussion has almost completely disregarded those of Rawls's arguments that are concerned with practicability, despite the significance accorded to them by Rawls himself. This article addresses the three most important of Rawls's objections against the practicability of utilitarianism: (1) that utilitarianism would generate too much disagreement to be politically workable, (2) that a utilitarian society would be vulnerable to social instability, and (3) that publicly adopting the principle of utility as the ultimate criterion of right and wrong would undermine the self-respect of some citizens. It is argued that Rawls's objections are either exaggerated or mistaken, and that this may have an impact on the assessment of `justice as fairness' as well as the utilitarian doctrine.
Naturally occurring short splice variant of CYLD positively regulates dendritic cell function
Authors: Srokowski Cathy Cecilia , Masri Joumana , Hövelmeyer Nadine , Krembel Anna Katharina , Tertilt Christine , Strand Dennis , Mahnke Karsten , Massoumi Ramin , Waisman Ari , Schild Hansjörg
Published: April 2009

Deubiquitination of NF-κB members by CYLD is crucial in controlling the magnitude and nature of cell activation. The role of the naturally occurring CYLD splice variant in dendritic cell (DC) function was analyzed using CYLDex7/8 mice, which lack the full-length CYLD (flCYLD) transcript and overexpress the short splice variant (sCYLD). Bone marrow–derived DCs from CYLDex7/8 mice display a hyperactive phenotype in vitro and in vivo and have a defect in establishing tolerance with the use of DEC-205–mediated antigen targeting to resting DCs. The combination of sCYLD overexpression and lack of flCYLD in CYLDex7/8 DCs leads to enhanced NF-κB activity accompanied by an increased nuclear translocation of the IκB molecule Bcl-3, along with nuclear p50 and p65. This suggests that, in contrast to flCYLD, sCYLD is a positive regulator of NF-κB activity, and its overexpression induces a hyperactive phenotype in DCs.
The evolution of isochore patterns in vertebrate genomes
Authors: Costantini Maria , Cammarano Rosalia , Bernardi Giorgio
Published: April 2009

Background

Previous work from our laboratory showed that (i) vertebrate genomes are mosaics of isochores, typically megabase-size DNA segments that are fairly homogeneous in base composition; (ii) isochores belong to a small number of families (five in the human genome) characterized by different GC levels; (iii) isochore family patterns are different in fishes/amphibians and mammals/birds, the latter showing GC-rich isochore families that are absent or very scarce in the former; (iv) there are two modes of genome evolution, a conservative one in which isochore patterns basically do not change (e.g., among mammalian orders), and a transitional one, in which they do change (e.g., between amphibians and mammals); and (v) isochores are tightly linked to a number of basic biological properties, such as gene density, gene expression, replication timing and recombination.

Results

The present availability of a number of fully sequenced genomes ranging from fishes to mammals allowed us to carry out investigations that (i) more precisely quantified our previous conclusions; (ii) showed that the different isochore families of vertebrate genomes are largely conserved in GC levels and dinucleotide frequencies, as well as in isochore size; and (iii) isochore family patterns can be either conserved or change within both warm- and cold-blooded vertebrates.

Conclusion

On the basis of the results presented, we propose that (i) the large conservation of GC levels and dinucleotide frequencies may reflect the conservation of chromatin structures; (ii) the conservation of isochore size may be linked to the role played by isochores in chromosome structure and replication; (iii) the formation, the maintainance and the changes of isochore patterns are due to natural selection.
Noncytotoxic orange and red/green derivatives of DsRed-Express2 for whole-cell labeling
Authors: Strack Rita L , Bhattacharyya Dibyendu , Glick Benjamin S , Keenan Robert J
Published: April 2009

Background

Whole-cell labeling is a common application of fluorescent proteins (FPs), but many red and orange FPs exhibit cytotoxicity that limits their use as whole-cell labels. Recently, a tetrameric red FP called DsRed-Express2 was engineered for enhanced solubility and was shown to be noncytotoxic in bacterial and mammalian cells. Our goal was to create derivatives of this protein with different spectral properties.

Results

Building on previous studies of DsRed mutants, we created two DsRed-Express2 derivatives: E2-Orange, an orange FP, and E2-Red/Green, a dual-color FP with both red and green emission. We show that these new FPs retain the low cytotoxicity of DsRed-Express2. In addition, we show that these new FPs are useful as second or third colors for flow cytometry and fluorescence microscopy.

Conclusion

E2-Orange and E2-Red/Green will facilitate the production of healthy, stably fluorescent cell lines and transgenic organisms for multi-color labeling studies.
Social determinants of health in Tunisia: the case-analysis of Ariana
Authors: Ben Romdhane Habiba , Grenier Francis R
Published: April 2009

Background

Few research projects have analyzed how social determinants of health impact cities in North Africa. The sustained growth in these countries has nevertheless proven to exacerbate health disparities and create many social and economic inequalities. This strategic analysis examines selected social determinants of health in a major urban centre of Tunisia, identifies the most influential stakeholders able to influence equity/inequity, and reviews the accomplishments and need for action to foster health equity.

Methods

This analysis was performed through a literature review and participatory research methods that included focus groups discussions and interview with key informants.

Results

Access to health care, changes in lifestyles, housing issues and gender-related inequities are prime, socially-determined elements that affect health in Ariana.

Conclusion

Recognition of emerging health issues is needed along with improved inter and intrasectoral coordination among stakeholders. The community-participatory approach used in this paper proved to be a useful scoping technique for this setting. A similar methodology could be used by other researchers as a first step toward health equity action at a city level.
Vsx2 in the zebrafish retina: restricted lineages through derepression
Authors: Vitorino Marta , Jusuf Patricia R , Maurus Daniel , Kimura Yukiko , Higashijima Shin-ichi , Harris William A
Published: April 2009

Background

The neurons in the vertebrate retina arise from multipotent retinal progenitor cells (RPCs). It is not clear, however, which progenitors are multipotent or why they are multipotent.

Results

In this study we show that the homeodomain transcription factor Vsx2 is initially expressed throughout the retinal epithelium, but later it is downregulated in all but a minor population of bipolar cells and all Müller glia. The Vsx2-negative daughters of Vsx2-positive RPCs divide and give rise to all other cell types in the retina. Vsx2 is a repressor whose targets include transcription factors such as Vsx1, which is expressed in the progenitors of distinct non-Vsx2 bipolars, and the basic helix-loop-helix transcription factor Ath5, which restricts the fate of progenitors to retinal ganglion cells, horizontal cells, amacrine cells and photoreceptors fates. Foxn4, expressed in the progenitors of amacrine and horizontal cells, is also negatively regulated by Vsx2.

Conclusion

Our data thus suggest Vsx2-positive RPCs are fully multipotent retinal progenitors and that when Vsx2 is downregulated, Vsx2-negative progenitors escape Vsx2 repression and so are able to express factors that restrict lineage potential.
Outcome after severe head injury: focal surgical lesions do not imply a better Glasgow Outcome Score than diffuse injuries at 3 months
Authors: Leach Paul , Pathmanaban Omar N , Patel Hiren C , Evans Julian , Sacho Raphael , Protheroe Richard , King Andrew T
Published: April 2009

Background

Historically neurosurgeons have accepted head injured patients only in the presence of a mass lesion requiring surgical decompression. Underpinning this is an assumption that these patients have a better outcome than patients without a surgical lesion. This has meant that many patients without a surgical lesion have been managed locally in the referring hospital. However, there is now evidence that treatment of all head injured patients in a specialist centre leads to improved outcomes. Therefore, we have asked the question: does the presence of a surgical lesion imply better outcome from severe head injury?

Results

We prospectively recorded the Glasgow Outcome score (GOS), at 3 months, of all the severely head injured patients treated at our institution over a two and a half year period. Of 116 patients admitted with an initial Glasgow Coma Score (GCS) of 8 or less, 58 had surgical lesions and 58 non-surgical head injuries. The two groups were well matched for presenting GCS and age. Overall our favourable outcome rate (GOS 4 and 5) at 3-months for the patients with a surgical lesion and for the non-surgical group were 47.3% and 46.6% respectively, with no significant difference between the two (P = 0.54).

Conclusion

The assumption in the past has always been that patients presenting in coma from traumatic diffuse brain injury will do worse than those that have a mass lesion amenable to surgical decompression. Our series would suggest that this is not the case and all severely head injured patients should expect similar outcome when cared for in a neuroscience centre.