bluuebunny/crossref_metadata_2025 huggingface dataset:
Duanzi as Networked Practice: How Online Satire Shapes Psychological Well-Being, Social Support, and Issue Knowledge for Chinese with Different Social Capital during COVID-19 Outbreaks
Authors: Pan Ji , Han Gang (Kevin) , Wei Ran
Published: September 2021

Practices oriented to digital technologies are being invented to change how people cope with crises. This study examines how Chinese netizens’ networked practices (e.g., liking, sharing, or commenting) with COVID-19 related duanzi (short online satires) influenced their psychological well-being, external social support, and issue knowledge during the pandemic. The role of social capital in moderating these relations is explored. Findings from the survey demonstrate that the act of “liking” a COVID-19 duanzi on WeChat has become a routine practice for Chinese netizens to kill time during the quarantine. However, the more bonding social capital one already had, the less they depended on duanzi “liking” to kill their boredom. Those less supported outside the family household, or less knowledgeable about the virus were also more likely to share a COVID-19 duanzi. Bonding social capital promotes one’s well-being, therefore, the positive psychological effect of duanzi sharing or commenting grows more pronounced for netizens with more bonding social capital. Bridging social capital brought external social support. Netizens with more bridging social capital obtained more external support and more COVID-19 knowledge from duanzi sharing. The theoretical and practical implications are elaborated in the conclusions.
Air Pollution during Pregnancy and Childhood Autism Spectrum Disorder in Taiwan
Authors: Wang Shu-Yuan , Cheng Ya-Yun , Guo How-Ran , Tseng Yen-Cheng
Published: September 2021

Air pollutants have been linked to some diseases in humans, but their effects on the nervous system were less frequently evaluated. Autism spectrum disorder (ASD) is a group of neurondevelopmental disorders of which the etiology is still unknown. We conducted a study in Taiwan to evaluate the possible associations between prenatal exposure to air pollutants and ASD. From a random sample of one million people in the National Insurance Research Database, we identified all the infants born between 1996 and 2000. We followed them till the end of 2013 and identified cases of ASD. We traced back the mothers’ residence and assessed the exposure to air pollutants using the data obtained from the air quality monitoring database maintained by the government, which included ozone (O3), carbon monoxide (CO), nitrogen dioxide (NO2), sulfur dioxide (SO2), and particulate matters with diameter less than 10 µm (PM10). Cox proportional hazard models were constructed to evaluate the associations between childhood ASD and exposures to the pollutants in the three trimesters and the whole gestation. We identified a total of 63,376 newborns and included 62,919 as the study cohort. After adjusting for other risk factors, we observed trimester-specific associations between levels of CO, NO2, and PM10 and the risk of childhood ASD. An increase of 1 ppm of CO in the first, second, and third trimester was associated with a hazard ratio (HR) of 1.93 (95% confidence interval [CI]: 1.55–2.39), 1.77 (95%CI: 1.41–2.22), and 1.75 (95%CI: 1.39–2.21), respectively. An increase of 10 ppb in the level of NO2 in the first, second, and third trimester was associated with an HR of 1.39 (95%CI: 1.22–1.58), 1.25 (95%CI: 1.10–1.42), and 1.18 (95%CI: 1.03–1.34), respectively. In conclusion, we found that exposures to CO and NO2 in all three trimesters were associated with increased risks of developing ASD.
Occupational Exposure to Ultrafine Particles in Metal Additive Manufacturing: A Qualitative and Quantitative Risk Assessment
Authors: Sousa Marta , Arezes Pedro , Silva Francisco
Published: September 2021

Ultrafine particles (UFPs) can be released unintentionally during metal additive manufacturing (AM). Experts agree on the urgent need to increase the knowledge of the emerging risk of exposure to nanoparticles, although different points of view have arisen on how to do so. This article presents a case study conducted on a metal AM facility, focused on studying the exposure to incidental metallic UFP. It intends to serve as a pilot study on the application of different methodologies to manage this occupational risk, using qualitative and quantitative approaches that have been used to study exposure to engineered nanoparticles. Quantitative data were collected using a condensation particle counter (CPC), showing the maximum particle number concentration in manual cleaning tasks. Additionally, scanning electron microscopy (SEM) and energy dispersive X-ray analyzer (EDS) measurements were performed, showing no significant change in the particles’ chemical composition, size, or surface (rugosity) after printing. A qualitative approach was fulfilled using Control Banding Nanotool 2.0, which revealed different risk bands depending on the tasks performed. This article culminates in a critical analysis regarding the application of these two approaches in order to manage the occupational risk of exposure to incidental nanoparticles, raising the potential of combining both.
Improving Well-Being of Farmers Using Ecological Awareness around Protected Areas: Evidence from Qinling Region, China
Authors: Ma Li , Qin Yueting , Zhang Han , Zheng Jie , Hou Yilei , Wen Yali
Published: September 2021

Sustainability of ecosystems is crucial for improving human well-being and sustainably developing human society. In recent years, global attention towards ecosystems and human well-being has been increasing. Exploring and understanding the relationship between ecosystems and human well-being, and establishing the well-being of residents while protecting the ecosystem have become urgent problems. Based on 618 valid samples collected from communities surrounding seven nature reserves in the Qinling Mountains region of China, this study analyzed the impact of ecosystem services on farmers’ well-being from the perspective of their subjective perception of ecosystem services by using multiple linear regression and seemingly unrelated regression methods. The main conclusions are as follows: supply of vegetation and clean water improves farmers’ well-being, improvement of air quality increases farmers’ life satisfaction, and the sense of belonging and tourism value brought by the ecosystem are important factors for farmers’ physical and mental pleasure and economic benefits. Therefore, the following countermeasures and suggestions are proposed: focusing on establishing the ecological well-being of farmers, improve implementation of the services and benefits provided by the ecosystem to farmers, increase publicity and education to improve the protection consciousness of farmers, and improve community participation mechanisms while mobilizing enthusiasm for protection. This article starts from the perspective of farmers’ perception, attempting to explore whether changes in ecosystem service functions will affect farmers’ well-being, so as to provide new opinions and suggestions for improving farmers’ well-being.
Ladder Use in Older People: Type, Frequency, Tasks and Predictors of Risk Behaviours
Authors: Hicks Cameron , Pliner Erika M. , Lord Stephen R. , Sturnieks Daina L.
Published: September 2021

Ladder fall and injury risk increases with age. People who present to a hospital after an injurious ladder fall have been surveyed, but little is known about ladder use in the community. The purpose of this study was to: (1) document salient factors related to ladder safety, and (2) determine physical, executive function, psychological and frequency-of-use factors associated with unsafe ladder use in older people. One hundred and two older people (aged 65+ years) were recruited. Participants completed questionnaires on demographics, health, and ladder use (type, frequency, task, behaviours) and underwent assessments of physical and executive function ability. Results showed both older men and women commonly use step ladders (61% monthly, 96% yearly), mostly inside the home for tasks such as changing a lightbulb (70%) and decorating (43%). Older men also commonly use straight ladders (27% monthly, 75% yearly), mostly outside the home for tasks such as clearing gutters (74%) and pruning trees (40%). Unsafe ladder use was more common in males and individuals with greater ladder use frequency, greater quadriceps strength, better upper limb dexterity, better balance, better stepping ability, greater self-reported everyday risk-taking, a lower fear of falling, and fewer health problems compared to their counterparts (all p < 0.05). These findings document ladder use by older people and provide insight into unsafe ladder behaviours that may be amenable to interventions to reduce ladder falls and associated injuries.
Traditional Masculinity and Aggression in Adolescence: Its Relationship with Emotional Processes
Authors: Malonda-Vidal Elisabeth , Samper-García Paula , Llorca-Mestre Anna , Muñoz-Navarro Roger , Mestre-Escrivá Vicenta
Published: September 2021

Traditional masculinity includes norms that encourage many of the aggressive behaviors whereas traditional femininity emphasizes aggression very little. In addition, the lack of emotional regulation as well as a poor impulse control have been related to aggression and, in particular, with reactive and proactive aggression. The objective of this study is to examine the role of gender stereotypes (masculinity/femininity) in reactive and proactive aggression, through regulatory emotional self-efficacy and emotion regulation. A total of 390 adolescents participated in a longitudinal study in Valencia, Spain. Structural equations modeling (SEM) was employed to explore a two-wave longitudinal model. The results show that femininity relates to reactive aggression through regulatory emotional self-efficacy and emotion regulation. This way, both emotional self-efficacy and emotional regulation mediate the relation between femininity and reactive aggression. Furthermore, reactive and proactive aggression relate positively and directly to masculinity and negatively to femininity. Therefore, violence prevention programs with adolescents should incorporate information to break down gender stereotypes and promote strategies to manage emotions. Such efforts may be helpful to reduce aggressive behaviors and violence.
Screening of Particulate Matter Reduction Ability of 21 Indigenous Korean Evergreen Species for Indoor Use
Authors: Jang Bo-Kook , Park Kyungtae , Lee Sang Yeob , Lee Hamin , Yeon Soo Ho , Ji Boran , Lee Cheol Hee , Cho Ju-Sung
Published: September 2021

The formation and pollution of particulate matter (PM), a side effect of rapid industrialization and urbanization, is considered a global issue. However, various plant species are able to effectively capture and reduce atmospheric PM concentrations. We investigated the indoor growth and morphology of 21 indigenous Korean evergreen species at low light intensities to ascertain their ability to reduce PM of aerosol particles in a closed acrylic chamber. The decrease in PM mass concentration differed significantly across species, with a significant correlation (8 h; p < 0.001). The reduction in the mass concentration of PM differed with particle size and across species. The highest reduction of PM2.5 occurred after 8 h with Dryopteris lacera (86.8%), Ilex × wandoensis (84.9%), Machilus thunbergii (84.3%), and Rhododendron brachycarpum (84.0%). Reduction of PM10 after 8 h was highest with Cephalotaxus harringtonii (98.3%), I. × wandoensis (98.5%), M. thunbergii (98.5%), and R. brachycarpum (98.3%). Plant morphological characteristics (category, plant height, leaf shape, leaf area) and relative humidity were closely related to the decrease in PM mass concentration. In conclusion, our findings can be used to identify Korean plant species that can reduce PM concentration and are suitable for indoor use.
Oral Health as a Predictor of Physical Frailty among Rural Community-Dwelling Elderly in an Agricultural County of Taiwan: A Cross-Sectional Study
Authors: Kuo Ya-Wen , Chen Mei-Yen , Chang Li-Ching , Lee Jiann-Der
Published: September 2021

We conducted a cross-sectional study to clarify the relationship between oral health and physical frailty (PF). A sample of 903 community-dwelling individuals aged ≥ 65 years were enrolled from random communities in Chiayi County. The self-perceived oral health (SPOH) and oral health assessment tool (OHAT), which consists of eight items, was used for the evaluation of their oral health status. PF was assessed based on the Study of Osteoporotic Fracture index. Overall, 14.6% of the participants had PF. In an adjusted model, restricted food types (odds ratio (OR) = 1.59, 95% confidence interval (CI): 1.2–2.09, p = 0.001), self-reported dental status (OR = 1.61, 95% CI: 1.2–2.15, p = 0.001), number of teeth (OR = 0.98, 95% CI: 0.96–0.99, p = 0.006), frequency of tooth cleaning (OR = 0.83, 95% CI: 0.68–1.0, p = 0.049), OHAT score (OR = 1.09, 95% CI: 1.02–1.17, p < 0.017), and saliva items of OHAT (OR = 1.52, 95% CI: 1.11–2.1, p = 0.010) were significantly associated with PF. SPOH is a crucial indicator of PF; longitudinal analyses are necessary to understand the underlying pathway of risk factors for frailty onset.
A 4 mm-Long Implant Rehabilitation in the Posterior Maxilla with Dynamic Navigation Technology: A Case Report after a Three-Years Post-Loading Follow-Up
Authors: Pellegrino Gerardo , Lizio Giuseppe , Rossi Fabio , Tuci Lorenzo , Ferraioli Lorenzo , Stefanelli Luigi Vito , Di Carlo Stefano , De Angelis Francesca
Published: September 2021

The use of short (<8 mm long) and ultra-short (<6 mm long) implants allows the prosthetic rehabilitation of the posterior ridges of the jaws avoiding reconstructive procedures. Nevertheless, this approach requires vast experience to ensure the primary stability of the fixture in a correct position. Computer-aided implantology (CAI) achieves better results than the free-hand one in terms of placement accuracy, reducing the surgical risks and the operative timings. Dynamic navigation (DN) allows the surgeon to track the position and movements of the drill in real-time on the CT imaging data set. It is more versatile than the computed static system, enabling the operator to change the guidance coordinates according to the intra-operative feedbacks. A mono-edentulous upper right first molar site was rehabilitated with a four mm-long implant to avoid reconstructive techniques, drastically rejected by the patients. The case was managed within a DN protocol considering the minimal available bone and the prosthetic demands. The phases of this procedure were strictly documented up to a 3-year follow-up. No intra-operative problems occurred, and adequate primary stability of the implant was obtained. The prosthetic loading was carried out within only six weeks without any complications. No variation of the baseline clinical scenario as evidenced clinically and radiographically at the end of follow-up. No similar cases are reported in the literature.
Taking Up and Terminating Leisure-Time Physical Activity over the Life Course: The Role of Life Events in the Familial and Occupational Life Domains
Authors: Lenze Lars , Klostermann Claudia , Lamprecht Markus , Nagel Siegfried
Published: September 2021

Leisure-time physical activity (LTPA) is associated with various health-promoting effects. However, little is known about the relationship between life events and changes in LTPA over the life course, especially when multiple life events occur simultaneously. Therefore, this study examines taking up and terminating LTPA associated with life events in the familial and occupational life domains over 16 years of 16–76-year-old Swiss inhabitants (n = 1857) in a retrospective longitudinal cohort design, using a validated telephone survey and multilevel discrete-time event-history analyses. The results show that taking up LTPA was more likely when ending a relationship and retiring and less likely when becoming a parent; terminating LTPA was more likely when ending a job, starting vocational training after 30 years, a relationship ended for men, and becoming a mother with increasing age. If experiencing multiple life events simultaneously, the greater the number of life events, the more likely persons aged 45–70 years were to take up LTPA and, conversely, the more likely persons aged 15–44 years to terminate LTPA. The relationship between life events and changes in LTPA over the life course was often age dependent, especially when experiencing multiple life events simultaneously. The findings should be considered when promoting LTPA.
A Possible Outbreak by Serratia marcescens: Genetic Relatedness between Clinical and Environmental Strains
Authors: Caggiano Giuseppina , Triggiano Francesco , Diella Giusy , Apollonio Francesca , Lopuzzo Marco , Mosca Adriana , Stolfa Stefania , Pazzani Carlo , Oliva Marta , Calia Carla , Laforgia Nicola , Dalfino Lidia , Barbuti Giovanna , Stefanizzi Pasquale , Minicucci Anna Maria , De Giglio Osvalda , Montagna Maria Teresa
Published: September 2021

Serratia marcescens (SM) is a Gram-negative bacterium that is frequently found in the environment. Since 1913, when its pathogenicity was first demonstrated, the number of infections caused by SM has increased. There is ample evidence that SM causes nosocomial infections in immunocompromised or critically ill patients admitted to the intensive care units (ICUs), but also in newborns admitted to neonatal ICUs (NICUs). In this study, we evaluated the possible genetic correlation by PFGE between clinical and environmental SM strains from NICU and ICU and compared the genetic profile of clinical strains with strains isolated from patients admitted to other wards of the same hospital. We found distinct clonally related groups of SM strains circulating among different wards of a large university hospital. In particular, the clonal relationship between clinical and environmental strains in NICU and ICU 1 was highlighted. The identification of clonal relationships between clinical and environmental strains in the wards allowed identification of the epidemic and rapid implementation of adequate measures to stop the spread of SM.
Relationship of Body Mass Index and Footprint Morphology to the Actual Height of the Medial Longitudinal Arch of the Foot
Authors: Rosende-Bautista Carolina , Munuera-Martínez Pedro V. , Seoane-Pillado Teresa , Reina-Bueno María , Alonso-Tajes Francisco , Pérez-García Sergio , Domínguez-Maldonado Gabriel
Published: September 2021

The medial longitudinal arch height of the foot is linked to individual characteristics such as sex and body mass index, and these characteristics have been shown to be associated with conditions such as flat feet. In this cross-sectional descriptive study, we examined the medial longitudinal arch morphology in an adult population to determine if there are differences related to sex and body mass index, and values were obtained for the foot posture index. Normalized anthropometric measurements and arch indices were calculated from footprints. Groups, defined by sex and body mass index, were compared, and the correlations between body mass index and the variables were determined. In the population studied (266 women and 177 men), significant differences between men and women for the foot posture index and normalized arch measurements were found. Analysis of the variables related to body mass index indicated there were significant differences in arch indices. Significant differences and positive correlations were also found between the arch index and body mass index for the left and right feet among the men and women studied. The results obtained allow us to reflect on and analyze whether the medial longitudinal arch morphology classification methods used in the clinical and research setting are adequate or whether the influence of factors such as body mass index can generate confusion.
Importance of the Working Environment for Early Retirement: Prospective Cohort Study with Register Follow-Up
Authors: Sundstrup Emil , Thorsen Sannie V. , Rugulies Reiner , Larsen Mona , Thomassen Kristina , Andersen Lars L.
Published: September 2021

Background: This study investigates the role of physical work demands and psychosocial work factors for early retirement among older workers. Methods: Data from three Danish surveys on work environment and health among employed older workers (age 55–59) were merged with a national register containing information on labour market participation. Robust Poisson regression modelled the risk ratios (RR) and 95% confidence intervals (CI) for the association between physical and psychosocial work factors and early retirement, that is, not working after the age of 64. Results: Of the 2800 workers, 53% retired early. High physical work demands (RR 1.33, 95% CI 1.19–1.48), poor overall psychosocial working conditions (RR 1.43, 95% CI 1.26–1.61), and access to early retirement benefits (RR 1.79, 95% CI 1.53–2.10) predicted early retirement. Subgroup analyses revealed that poor overall psychosocial working conditions were a stronger predictor for early retirement among workers with seated jobs than those with physically active jobs. Conclusions: High physical work demands and poor psychosocial working conditions are factors that can push older workers out of the labour market prematurely. Poor psychosocial working conditions seem to be a particularly strong push factor among workers with seated work.
Gender Differences in Nutritional Intake among Rural-Urban Migrants in China
Authors: Sun Qian , Li Xiaoyun , Rahut Dil Bahadur
Published: September 2021

Due to rapid economic growth and urbanization, China has witnessed massive migration from rural to urban areas and significant changes in food habits and nutritional intake. This paper empirically examines the factors affecting nutritional intake of 7752 rural-urban migrants and its gender effects, using the China Health and Nutrition Survey (CHNS) data from 1991 to 2011. The descriptive results show that female migrants (FMs) have lower energy intake but have higher proportions of energy from protein and fat than male migrants (MMs), indicating that FMs have a better nutritional quality. The regression results show that the calorie-income elasticities are 0.014 for MMs and 0.018 for FMs. A remarkable positive effect of income on nutritional quality is found for FMs. Employment in non-farm sectors and the community environmental sanitation have a significant increase in calorie intake and nutritional quality, while non-farm employment presents a negative and significant effect on FMs’ calorie intake. Modern market significantly positively affects migrants’ energy intake from protein, thereby improving their nutritional quality. The exogenous switching treatment effect regression results display a significant gender effect on energy intake and its share from protein for migrants, while there is no significant gender effect when it comes to the share of energy from fat.
Effects of the COVID-19 Mitigation Measures on Alcohol Consumption and Binge Drinking in College Students: A Longitudinal Survey
Authors: Vasconcelos Margarida , Crego Alberto , Rodrigues Rui , Almeida-Antunes Natália , López-Caneda Eduardo
Published: September 2021

To “flatten the curve” of COVID-19 contagion, several countries ordered lockdowns amid the pandemic along with indications on social distancing. These social isolation measures could potentially bring alterations to healthy behavior, including to alcohol consumption. However, there is hardly any scientific evidence of the impact of such measures on alcohol consumption and binge drinking (BD) among young adults, and how they relate to alcohol craving, stress, anxiety, and depression levels. We addressed these questions by conducting a longitudinal study with 146 Portuguese college students—regular binge drinkers (regular BDs), infrequent binge drinkers (infrequent BDs) and non-binge drinkers (non-BDs)—in three moments: before the pandemic (Pre-Lockdown), during lockdown (Lockdown) and 6 months after (Post-Lockdown). Results revealed that regular BDs decreased alcohol use during Lockdown, a change in behavior that was even greater during Post-Lockdown, when regular BDs displayed similar levels of consumption to infrequent/non-BDs. Additionally, alcohol craving and living with friends were predictive of alcohol use during Lockdown, whereas stress, anxiety, and depression symptoms did not contribute to explain changes in drinking behavior. Collectively, the results suggest that BD in young Portuguese college students can be stopped when the contexts in which alcohol intake usually takes place are suppressed, which may have important implications for future prevention and intervention strategies.
Cooking, Shopping, and Eating Behaviors of African American and Hispanic Families: Implications for a Culturally Appropriate Meal Kit Intervention
Authors: Sweeney Lauren H. , Carman Kaley , Varela Elder G. , House Lisa A. , Shelnutt Karla P.
Published: September 2021

Families with low incomes face barriers to preparing healthy meals, including decreased food access and limited time, and may turn to fast, low-quality, and inexpensive foods. Affordable and accessible meal kits may reduce these barriers. The objective of this study was to explore the cooking, eating, and shopping behaviors of African American (AA) and Hispanic participants living in the United States with low incomes and determine the knowledge of and preferences for a culturally appropriate meal kit intervention. Trained researchers conducted focus groups using a semi-structured questionnaire with AA and Hispanic food preparers with low incomes. Participant cooking, eating, and shopping behaviors and knowledge of and preferences for a culturally appropriate meal kit intervention were evaluated using thematic analysis. AA participants (n = 16) reported cooking on average 2 to 3 days per week and more often on weekends. Hispanic participants (n = 15) reported cooking 5 days per week and more often during the week. Both groups identified cost as the number one consideration when shopping. Most were unfamiliar with meal kits but indicated they would try an affordable meal kit. AA and Hispanic participants differed in their cooking, eating, and shopping behaviors but were equally interested in trying meal kits if affordable and culturally appropriate.
The Measurement of Food Insecurity in High-Income Countries: A Scoping Review
Authors: Carrillo-Álvarez Elena , Salinas-Roca Blanca , Costa-Tutusaus Lluís , Milà-Villarroel Raimon , Shankar Krishnan Nithya
Published: September 2021

The measurement of food insecurity is essential to monitor the prevalence, risk factors, consequences and effects of food insecurity and the interventions and policies implemented to tackle it. Yet, how best to apply it remains an unsettled issue due to the multifaceted and context-dependent nature of food insecurity. We report a scoping review of measures of food insecurity at the individual and household level in high-income countries with the final purpose of facilitating a catalogue of instruments to be used by both researchers and practitioners. The scoping review was conducted following the methodological framework of Arksey and O’Malley and the Joanna Briggs Institute guidelines. We included all types of documents published between 2000–2020 using instruments that estimate food insecurity at both individual and household level in high-income countries, and with respondents including adolescents, adults, and elderly. We identified a total of 23 measurement strategies being used in 33 peer-reviewed publications and 114 documents from the grey literature. Our results show that most measures focus on the access dimension of food insecurity and that further research is required to develop measures that incorporate aspects of quality of dietary intake and relevant individual, household and social conditions related to food insecurity.
Estimated Direct Medical Cost of Osteoporosis in Saudi Arabia: A Single-Center Retrospective Cost Analysis
Authors: Balkhi Bander , Alghamdi Ahmed , Alqusair Sulaiman , Alotaibi Bader , AlRuthia Yazed , Alsanawi Hisham , Nasser Ahmad Bin , Fouda Mona A.
Published: September 2021

Osteoporosis and its complications are a major health concern in Saudi Arabia, and the prevalence of osteoporosis is on the rise. The aim of this study was to estimate the direct healthcare cost for patients with osteoporosis. A retrospective study was carried out among adult patients with osteoporosis in a teaching hospital in Saudi Arabia. A bottom-up approach was conducted to estimate the healthcare resources used and the total direct medical cost for the treatment of osteoporosis and related fractures. The study included 511 osteoporosis patients, 93% of whom were female. The average (SD) age was 68.5 years (10.2). The total mean direct medical costs for patients without fractures were USD 975.77 per person per year (PPPY), and for those with osteoporotic fractures, the total direct costs were USD 9716.26 PPPY, of which 56% of the costs were attributable to surgery procedures. Prior to fractures, the main cost components were medication, representing 61%, and physician visits, representing 18%. The findings of this study indicated the economic impact of osteoporosis and related fractures. With the aging population in Saudi Arabia, the burden of disease could increase significantly, which highlights the need for effective prevention strategies to minimize the economic burden of osteoporosis.
Investigating Eco-Environmental Vulnerability for China–Pakistan Economic Corridor Key Sector Punjab Using Multi-Sources Geo-Information
Authors: Kamran Muhammad , Bian Jinhu , Li Ainong , Lei Guangbin , Nan Xi , Jin Yuan
Published: September 2021

China-Pakistan economic corridor (CPEC), a critical part of the Belt and Road initiative (BRI), is subjected to rapid infrastructure development, which may lead to potential eco-environmental vulnerability. This study uses multi-source geo-information, and the multi-criteria decision-making (MCDM)-based best–worst method (BWM) to quantify the baseline eco-environmental vulnerability of one key CPEC sector—the Punjab province. The Punjab province is an important connection between northern and southern CPEC routes in Pakistan. In this study, we have established an indicator system consisting of twenty-two influential factors in a geospatial database to conduct eco-environmental vulnerability analysis. The overall setup is supported by a geographic information system (GIS) to perform spatial analysis. The resulting map was categorized into five vulnerability levels: very low, low, medium, high, and very high. The results revealed that the overall eco-environmental health of the Punjab province is reasonably good as 4.64% and 59.45% area of the key sector lies in ‘very low’ and ‘low’ vulnerability categories; however, there also exist highly vulnerable areas, particularly in the proximity of CPEC projects. Although high vulnerability areas constitute a very small percentage, only 0.08% of the Punjab province, still, decision-makers need to be aware of those regions and make corresponding protection strategies. Our study demonstrated that the MCDM-BWM-based EVA model could be effectively used to quantify vulnerability in other areas of CPEC. The findings of the study emphasize that management policies should be aligned with research-based recommendations for ecological protection, natural resource utilization, and sustainable development in regions participating in BRI.
Assessing Place Type Similarities Based on Functional Signatures Extracted from Social Media Data
Authors: Oh Doori , Yao Xiaobai A.
Published: September 2021

Place types are often used to query places or retrieve data in gazetteers. Existing gazetteers do not use the same place type classification schemes, and the various typing schemes can cause difficulties in data alignment and matching. Different place types may share some level of similarities. However, previous studies have paid little attention to the place type similarities. This study proposes an analytical approach to measuring similarities between place types in multiple typing schemes based on functional signatures extracted from web-harvested place descriptions. In this study, a functional signature consists of three component signature factors: place affordance, events, and key-descriptors. The proposed approach has been tested in a case study using Twitter data. The case study finds high similarity scores between some pairs of types and summarizes the situations when high similarities could occur. The research makes two innovative contributions: First, it proposes a new analytical approach to measuring place type similarities. Second, it demonstrates the potential and benefits of using location-based social media data to better understand places.
Metformin and Dichloroacetate Suppress Proliferation of Liver Cancer Cells by Inhibiting mTOR Complex 1
Authors: Kim Tae Suk , Lee Minjong , Park Minji , Kim Sae Yun , Shim Min Suk , Lee Chea Yeon , Choi Dae Hee , Cho Yuri
Published: September 2021

The Warburg effect is important for cancer cell proliferation. This phenomenon can be flexible by interaction between glycolysis and mitochondrial oxidation for energy production. We aimed to investigate the anticancer effects of the pyruvate dehydrogenase kinase inhibitor, dichloroacetate (DCA) and the mitochondrial respiratory complex I inhibitor metformin in liver cancer cells. The anticancer effect of DCA and/or metformin on HepG2, PLC/PRF5 human liver cancer cell lines, MH-134 murine hepatoma cell lines, and primary normal hepatocytes using MTT assay. Inhibition of lactate/ATP production and intracellular reactive oxygen species generation by DCA and metformin was investigated. Inhibition of PI3K/Akt/mTOR complex I was evaluated to see whether it occurred through AMPK signaling. Anticancer effects of a combination treatment of DCA and metformin were evaluated in HCC murine model. The results showed that metformin and DCA effectively induced apoptosis in liver cancer cells. A combination treatment of metformin and DCA did not affect viability of primary normal hepatocytes. Metformin upregulated glycolysis in liver cancer cells, thereby increasing sensitivity to the DCA treatment. Metformin and DCA inhibited mTOR complex I signaling through upregulated AMPK-independent REDD1. In addition, metformin and DCA increased reactive oxygen species levels in liver cancer cells, which induced apoptosis. A combination treatment of metformin and DCA significantly suppressed the tumor growth of liver cancer cells using in vivo xenograft model. Taken together, the combined treatment of metformin and DCA suppressed the growth of liver cancer cells. This strategy may be effective for patients with advanced liver cancer.
Extracellular Calcium Ion Concentration Regulates Chondrocyte Elastic Modulus and Adhesion Behavior
Authors: Shen Xingyu , Hu Liqiu , Li Zhen , Wang Liyun , Pang Xiangchao , Wen Chun-Yi , Tang Bin
Published: September 2021

Extracellular calcium ion concentration levels increase in human osteoarthritic (OA) joints and contribute to OA pathogenesis. Given the fact that OA is a mechanical problem, the effect of the extracellular calcium level ([Ca2+]) on the mechanical behavior of primary human OA chondrocytes remains to be elucidated. Here, we measured the elastic modulus and cell–ECM adhesion forces of human primary chondrocytes with atomic force microscopy (AFM) at different extracellular calcium ion concentration ([Ca2+]) levels. With the [Ca2+] level increasing from the normal baseline level, the elastic modulus of chondrocytes showed a trend of an increase and a subsequent decrease at the level of [Ca2+], reaching 2.75 mM. The maximum increment of the elastic modulus of chondrocytes is a 37% increase at the peak point. The maximum unbinding force of cell-ECM adhesion increased by up to 72% at the peak point relative to the baseline level. qPCR and immunofluorescence also indicated that dose-dependent changes in the expression of myosin and integrin β1 due to the elevated [Ca2+] may be responsible for the variations in cell stiffness and cell-ECM adhesion. Scratch assay showed that the chondrocyte migration ability was modulated by cell stiffness and cell-ECM adhesion: as chondrocyte’s elastic modulus and cell-ECM adhesion force increased, the migration speed of chondrocytes decreased. Taken together, our results showed that [Ca2+] could regulate chondrocytes stiffness and cell-ECM adhesion, and consequently, influence cell migration, which is critical in cartilage repair.
Quinolone Resistance of Actinobacillus pleuropneumoniae Revealed through Genome and Transcriptome Analyses
Authors: Ma Xiaoping , Zheng Bowen , Wang Jiafan , Li Gen , Cao Sanjie , Wen Yiping , Huang Xiaobo , Zuo Zhicai , Zhong Zhijun , Gu Yu
Published: September 2021

Actinobacillus pleuropneumoniae is a pathogen that infects pigs and poses a serious threat to the pig industry. The emergence of quinolone-resistant strains of A.pleuropneumoniae further limits the choice of treatment. However, the mechanisms behind quinolone resistance in A.pleuropneumoniae remain unclear. The genomes of a ciprofloxacin-resistant strain, A. pleuropneumoniae SC1810 and its isogenic drug-sensitive counterpart were sequenced and analyzed using various bioinformatics tools, revealing 559 differentially expressed genes. The biological membrane, plasmid-mediated quinolone resistance genes and quinolone resistance-determining region were detected. Upregulated expression of efflux pump genes led to ciprofloxacin resistance. The expression of two porins, OmpP2B and LamB, was significantly downregulated in the mutant. Three nonsynonymous mutations in the mutant strain disrupted the water–metal ion bridge, subsequently reducing the affinity of the quinolone–enzyme complex for metal ions and leading to cross-resistance to multiple quinolones. The mechanism of quinolone resistance in A. pleuropneumoniae may involve inhibition of expression of the outer membrane protein genes ompP2B and lamB to decrease drug influx, overexpression of AcrB in the efflux pump to enhance its drug-pumping ability, and mutation in the quinolone resistance-determining region to weaken the binding of the remaining drugs. These findings will provide new potential targets for treatment.
Discovering the DNA-Binding Consensus of the Thermus thermophilus HB8 Transcriptional Regulator TTHA1359
Authors: Teague Josiah L. , Barrows John K. , Baafi Cynthia A. , Van Dyke Michael W.
Published: September 2021

Transcription regulatory proteins, also known as transcription factors, function as molecular switches modulating the first step in gene expression, transcription initiation. Cyclic-AMP receptor proteins (CRPs) and fumarate and nitrate reduction regulators (FNRs) compose the CRP/FNR superfamily of transcription factors, regulating gene expression in response to a spectrum of stimuli. In the present work, a reverse-genetic methodology was applied to the study of TTHA1359, one of four CRP/FNR superfamily transcription factors in the model organism Thermus thermophilus HB8. Restriction Endonuclease Protection, Selection, and Amplification (REPSA) followed by next-generation sequencing techniques and bioinformatic motif discovery allowed identification of a DNA-binding consensus for TTHA1359, 5′–AWTGTRA(N)6TYACAWT–3′, which TTHA1359 binds to with high affinity. By bioinformatically mapping the consensus to the T. thermophilus HB8 genome, several potential regulatory TTHA1359-binding sites were identified and validated in vitro. The findings contribute to the knowledge of TTHA1359 regulatory activity within T. thermophilus HB8 and demonstrate the effectiveness of a reverse-genetic methodology in the study of putative transcription factors.
Transcriptomic Analysis of the Differential Nephrotoxicity of Diverse Brominated Flame Retardants in Rat and Human Renal Cells
Authors: Barnett Lillie Marie A. , Kramer Naomi E. , Buerger Amanda N. , Love Deirdre H. , Bisesi Joseph H. , Cummings Brian S.
Published: September 2021

Brominated flame retardants (BFRs) are environmentally persistent, are detected in humans, and some have been banned due to their potential toxicity. BFRs are developmental neurotoxicants and endocrine disruptors; however, few studies have explored their potential nephrotoxicity. We addressed this gap in the literature by determining the toxicity of three different BFRs (tetrabromobisphenol A (TBBPA), hexabromocyclododecane (HBCD), and tetrabromodiphenyl ether (BDE-47)) in rat (NRK 52E) and human (HK-2 and RPTEC) tubular epithelial cells. All compounds induced time- and concentration-dependent toxicity based on decreases in MTT staining and changes in cell and nuclear morphology. The toxicity of BFRs was chemical- and cell-dependent, and human cells were more susceptible to all three BFRs based on IC50s after 48 h exposure. BFRs also had chemical- and cell-dependent effects on apoptosis as measured by increases in annexin V and PI staining. The molecular mechanisms mediating this toxicity were investigated using RNA sequencing. Principal components analysis supported the hypothesis that BFRs induce different transcriptional changes in rat and human cells. Furthermore, BFRs only shared nine differentially expressed genes in rat cells and five in human cells. Gene set enrichment analysis demonstrated chemical- and cell-dependent effects; however, some commonalities were also observed. Namely, gene sets associated with extracellular matrix turnover, the coagulation cascade, and the SNS-related adrenal cortex response were enriched across all cell lines and BFR treatments. Taken together, these data support the hypothesis that BFRs induce differential toxicity in rat and human renal cell lines that is mediated by differential changes in gene expression.
Identification and Characterization of Novel circRNAs Involved in Muscle Growth of Blunt Snout Bream (Megalobrama amblycephala)
Authors: Liu Lifang , Chen Yulong , Diao Jinghan , Luo Lifei , Gao Zexia
Published: September 2021

Circular RNAs (circRNAs), a novel class of endogenous RNAs, have been recognized to play important roles in the growth of animals. However, the regulatory mechanism of circRNAs on fish muscle growth is still unclear. In this study, we performed whole transcriptome analysis of skeletal muscles from two populations with different growth rates (fast-growing and slow-growing) of blunt snout bream (Megalobrama amblycephala), an important fish species for aquaculture. The selected circRNAs were validated by qPCR and Sanger sequencing. Pairs of circRNA–miRNA–mRNA networks were constructed with the predicted differentially expressed (DE) pairs, which revealed regulatory roles in muscle myogenesis and hypertrophy. As a result, a total of 445 circRNAs were identified, including 42 DE circRNAs between fast-growing (FG) and slow-growing (SG) groups. Many of these DE circRNAs were related with aminoglycan biosynthetic and metabolic processes, cytokinetic processes, and the adherens junction pathway. The functional prediction results showed that novel_circ_0001608 and novel_circ_0002886, competing to bind with dre-miR-153b-5p and dre-miR-124-6-5p, might act as competing endogenous RNAs (ceRNAs) to control MamblycephalaGene14755 (pik3r1) and MamblycephalaGene10444 (apip) level, respectively, thus playing an important regulatory role in muscle growth. Overall, these results will not only help us to further understand the novel RNA transcripts in M. amblycephala, but also provide new clues to investigate the potential mechanism of circRNAs regulating fish growth and muscle development.
Study on Maximum Specific Loss Power in Fe3O4 Nanoparticles Decorated with Biocompatible Gamma-Cyclodextrins for Cancer Therapy with Superparamagnetic Hyperthermia
Authors: Caizer Costica , Caizer Isabela Simona
Published: September 2021

Different chemical agents are used for the biocompatibility and/or functionality of the nanoparticles used in magnetic hyperthermia to reduce or even eliminate cellular toxicity and to limit the interaction between them (van der Waals and magnetic dipolar interactions), with highly beneficial effects on the efficiency of magnetic hyperthermia in cancer therapy. In this paper we propose an innovative strategy for the biocompatibility of these nanoparticles using gamma-cyclodextrins (γ-CDs) to decorate the surface of magnetite (Fe3O4) nanoparticles. The influence of the biocompatible organic layer of cyclodextrins, from the surface of Fe3O4 ferrimagnetic nanoparticles, on the maximum specific loss power in superparamagnetic hyperthermia, is presented and analyzed in detail in this paper. Furthermore, our study shows the optimum conditions in which the magnetic nanoparticles covered with gamma-cyclodextrin (Fe3O4–γ-CDs) can be utilized in superparamagnetic hyperthermia for an alternative cancer therapy with higher efficiency in destroying tumoral cells and eliminating cellular toxicity.
Analysis of Molecular Networks in the Cerebellum in Chronic Schizophrenia: Modulation by Early Postnatal Life Stressors in Murine Models
Authors: Vera-Montecinos América , Rodríguez-Mias Ricard , MacDowell Karina S. , García-Bueno Borja , Bris Álvaro G. , Caso Javier R. , Villén Judit , Ramos Belén
Published: September 2021

Despite the growing importance of the cerebellum as a region highly vulnerable to accumulating molecular errors in schizophrenia, limited information is available regarding altered molecular networks with potential therapeutic targets. To identify altered networks, we conducted one-shot liquid chromatography–tandem mass spectrometry in postmortem cerebellar cortex in schizophrenia and healthy individuals followed by bioinformatic analysis (PXD024937 identifier in ProteomeXchange repository). A total of 108 up-regulated proteins were enriched in stress-related proteins, half of which were also enriched in axonal cytoskeletal organization and vesicle-mediated transport. A total of 142 down-regulated proteins showed an enrichment in proteins involved in mitochondrial disease, most of which were also enriched in energy-related biological functions. Network analysis identified a mixed module of mainly axonal-related pathways for up-regulated proteins with a high number of interactions for stress-related proteins. Energy metabolism and neutrophil degranulation modules were found for down-regulated proteins. Further, two double-hit postnatal stress murine models based on maternal deprivation combined with social isolation or chronic restraint stress were used to investigate the most robust candidates of generated networks. CLASP1 from the axonal module in the model of maternal deprivation was combined with social isolation, while YWHAZ was not altered in either model. METTL7A from the degranulation pathway was reduced in both models and was identified as altered also in previous gene expression studies, while NDUFB9 from the energy network was reduced only in the model of maternal deprivation combined with social isolation. This work provides altered stress- and mitochondrial disease-related proteins involved in energy, immune and axonal networks in the cerebellum in schizophrenia as possible novel targets for therapeutic interventions and suggests that METTL7A is a possible relevant altered stress-related protein in this context.