bluuebunny/crossref_metadata_2025 huggingface dataset:
Time-Lag Effect of Climate Conditions on Vegetation Productivity in a Temperate Forest–Grassland Ecotone
Authors: Liu Xinyue , Tian Yun , Liu Shuqin , Jiang Lixia , Mao Jun , Jia Xin , Zha Tianshan , Zhang Kebin , Wu Yuqing , Zhou Jianqin
Published: June 2022

Climate conditions can significantly alter the vegetation net primary productivity (NPP) in many of Earth’s ecosystems, although specifics of NPP–climate condition interactions, especially time-lag responses on seasonal scales, remain unclear in ecologically sensitive forest–grassland ecotones. Based on the Moderate-Resolution Imaging Spectroradiometer (MODIS) and meteorological datasets, we analyzed the relationship between NPP and precipitation, temperature, and drought during the growing season (April–August), considering the time-lag effect (0–5 months) at the seasonal scale in Hulunbuir, Inner Mongolia, China from 2000 to 2018. The results revealed a delayed NPP response to precipitation and drought throughout the growing season. In April, the precipitation in the 4 months before (i.e., the winter of the previous year) explained the variation in NPP. In August, the NPP in some areas was influenced by the preceding 1~2 months of drought. The time-lag effect varied with vegetation type and soil texture at different spatial patterns. Compared to grass and crop, broadleaf forest and meadow exhibited a longer legacy of precipitation during the growing season. The length of the time-lag effects of drought on NPP increased with increasing soil clay content during the growing season. The interaction of vegetation types and soil textures can explain 37% of the change in the time-lag effect of the NPP response to PPT on spatial pattern. Our findings suggested that preceding precipitation influences vegetation growth at the early stages of growth, while preceding drought influences vegetation growth in the later stages of growth. The spatial pattern of the time lag was significantly influenced by interaction between vegetation type and soil texture factors. This study highlights the importance of considering the time-lag effects of climate conditions and underlying drivers in further improving the prediction accuracy of NPP and carbon sinks in temperate semiarid forest–grassland ecotones.
Comparative Analysis of Flavor, Taste, and Volatile Organic Compounds in Opossum Shrimp Paste during Long-Term Natural Fermentation Using E-Nose, E-Tongue, and HS-SPME-GC-MS
Authors: Deng Yijia , Wang Rundong , Zhang Yuhao , Li Xuepeng , Gooneratne Ravi , Li Jianrong
Published: June 2022

The present study focused on the determination of color, flavor, taste, and volatile organic compounds (VOCs) changes of shrimp paste fermented for 1, 2, 3, and 8 years by E-nose, E-tongue, and headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS). During fermentation, the color of shrimp paste turned dark brown with decreases in L*, a*, and b* values. Inorganic sulfide odor was dominant in all fermented samples. The umami, richness, and aftertaste-B reached a maximum in year 3 of fermentation. A total of 182 volatiles, including long-chain alkanes, esters, aldehydes, olefins, ketones, acids, furans, and pyrazines, were detected. Sixteen VOCs including dimethyl disulfide, methional, trimethyl-pyrazine, (E,E)-2,4-heptadienal, benzeneacetaldehyde were selected as flavor markers. Correlation analysis showed that 94 VOCs were related to saltiness while 40, 17, 21, 22, and 24 VOCs contributed to richness, umami, aftertase-B, sourness, and bitterness, respectively. These novel data may help in optimizing fermentation duration to achieve target flavor indicators in opossum shrimp paste production.
Semantics of Dairy Fermented Foods: A Microbiologist’s Perspective
Authors: Vitali Francesco , Zinno Paola , Schifano Emily , Gori Agnese , Costa Ana , De Filippo Carlotta , Koroušić Seljak Barbara , Panov Panče , Devirgiliis Chiara , Cavalieri Duccio
Published: June 2022

Food ontologies are acquiring a central role in human nutrition, providing a standardized terminology for a proper description of intervention and observational trials. In addition to bioactive molecules, several fermented foods, particularly dairy products, provide the host with live microorganisms, thus carrying potential “genetic/functional” nutrients. To date, a proper ontology to structure and formalize the concepts used to describe fermented foods is lacking. Here we describe a semantic representation of concepts revolving around what consuming fermented foods entails, both from a technological and health point of view, focusing actions on kefir and Parmigiano Reggiano, as representatives of fresh and ripened dairy products. We included concepts related to the connection of specific microbial taxa to the dairy fermentation process, demonstrating the potential of ontologies to formalize the various gene pathways involved in raw ingredient transformation, connect them to resulting metabolites, and finally to their consequences on the fermented product, including technological, health and sensory aspects. Our work marks an improvement in the ambition of creating a harmonized semantic model for integrating different aspects of modern nutritional science. Such a model, besides formalizing a multifaceted knowledge, will be pivotal for a rich annotation of data in public repositories, as a prerequisite to generalized meta-analysis.
Modelling the Adhesion and Biofilm Formation Boundary of Listeria monocytogenes ST9
Authors: Hu Lili , Dong Qingli , Li Zhuosi , Ma Yue , Aslam Muhammad Zohaib , Liu Yangtai
Published: June 2022

Listeria monocytogenes is a major foodborne pathogen that can adhere to or form a biofilm on food contact surfaces, depending on the environmental conditions. The purpose of this work is to determine the adhesion and biofilm formation boundaries for L. monocytogenes ST9 under the combination environments of temperature (5, 15, and 25 °C), NaCl concentration (0%, 3%, 6%, and 9% (w/v)) and pH (5.0, 6.0, 7.0, and 8.0). The probability models of adhesion and biofilm formation were built using the logistic regression. For adhesion, only the terms of linear T and NaCl are significant for L. monocytogenes ST9 (p < 0.05), whereas the terms of linear T, NaCl, and pH, and the interaction between T and pH were significant for biofilm formation (p < 0.05). By analyzing contour maps and their surface plots for two different states, we discovered that high temperature promoted adhesion and biofilm formation, whereas excessive NaCl concentration inhibited both of them. With a stringent threshold of 0.1667, the accuracy rate for identifying both adhesion/no-adhesion and biofilm formation/no-biofilm formation events were 0.929, indicating that the probability models are reasonably accurate in predicting the adhesion and biofilm formation boundary of L. monocytogenes ST9. The boundary model may provide a useful way for determining and further controlling L. monocytogenes adhesion and biofilm formation in various food processing environments.
Celery Root Phenols Content, Antioxidant Capacities and Their Correlations after Osmotic Dehydration in Molasses
Authors: Nićetin Milica , Pezo Lato , Pergal Marija , Lončar Biljana , Filipović Vladimir , Knežević Violeta , Demir Hande , Filipović Jelena , Manojlović Dragan
Published: June 2022

The osmotic dehydration (OD) of celery root in sugar beet molasses was studied at three temperatures (20, 35, and 50 °C) and three immersion periods (1, 3, and 5 h) in order to examine the changes in antioxidant potential and phenolic profile of celery root throughout the process. The antioxidant capacity (AOC) of dehydrated samples was evaluated by spectrophotometric and polarographic assays, the total phenolic content by the Folin-Ciocalteu method, and the individual phenolic compounds by HPLC-DAD. As a result of OD in molasses, the AOC and phenols content in samples increased proportionally to the augmentation of temperature and the immersion time. Vanillic acid, syringic acid, and catechin were detected in dehydrated samples as a result of transfer from molasses. Compared to fresh celery root, the content of identified phenols in osmodehydrated samples was improved from 1.5 to 6.2 times. Strong correlations between applied assays were obtained, except for the DPPH. Based on the correlation analysis chlorogenic acid, gallic acid, chrysin, catechin, and kaempferol showed the greatest contribution to the overall AOC of osmodehydrated celery root. Molasses, an agro-industrial waste from sugar production, could be valorized as a valuable osmotic solution.
Modeling and Numerical Simulation for Covering the Fractional COVID-19 Model Using Spectral Collocation-Optimization Algorithm
Authors: Khader Mohamed M. , Adel Mohamed
Published: June 2022

A primary aim of this study is to examine and simulate a fractional Coronavirus disease model by providing an efficient method for solving numerically this important model. In the Liouville-Caputo sense, the examined model consists of five fractional-order differential equations. With the Vieta-Lucas spectral collocation method, the unknown functions can be discretized and fractional derivatives can be obtained. With the system of nonlinear algebraic equations obtained, we can simplify the examined problem. In this system, the unknown coefficients are discovered by constructing and solving it as a restricted optimization problem. Some theoretical investigations are stated to examine the convergence analysis and stability analysis of the proposed approach and model. The results produced using the fractional finite difference technique (FDM), where the fractional differentiation operator was discretized using the Grünwald-Letnikov approach, are compared. The FDM relies heavily upon accurately turning the proposed model into a system of algebraic equations. To assess the algorithm’s correctness and usefulness, a numerical simulation is included.
Genetic Analysis Algorithm for the Study of Patients with Multiple Congenital Anomalies and Isolated Congenital Heart Disease
Authors: Delea Marisol , Massara Lucia S. , Espeche Lucia D. , Bidondo María Paz , Barbero Pablo , Oliveri Jaen , Brun Paloma , Fabro Mónica , Galain Micaela , Fernández Cecilia S. , Taboas Melisa , Bruque Carlos D. , Kolomenski Jorge E. , Izquierdo Agustín , Berenstein Ariel , Cosentino Viviana , Martinoli Celeste , Vilas Mariana , Rittler Mónica , Mendez Rodrigo , Furforo Lilian , Liascovich Rosa , Groisman Boris , Rozental Sandra , Dain Liliana ,   on behalf of the PID ACM-CC Group
Published: June 2022

Congenital anomalies (CA) affect 3–5% of newborns, representing the second-leading cause of infant mortality in Argentina. Multiple congenital anomalies (MCA) have a prevalence of 2.26/1000 births in newborns, while congenital heart diseases (CHD) are the most frequent CA with a prevalence of 4.06/1000 births. The aim of this study was to identify the genetic causes in Argentinian patients with MCA and isolated CHD. We recruited 366 patients (172 with MCA and 194 with isolated CHD) born between June 2015 and August 2019 at public hospitals. DNA from peripheral blood was obtained from all patients, while karyotyping was performed in patients with MCA. Samples from patients presenting conotruncal CHD or DiGeorge phenotype (n = 137) were studied using MLPA. Ninety-three samples were studied by array-CGH and 18 by targeted or exome next-generation sequencing (NGS). A total of 240 patients were successfully studied using at least one technique. Cytogenetic abnormalities were observed in 13 patients, while 18 had clinically relevant imbalances detected by array-CGH. After MLPA, 26 patients presented 22q11 deletions or duplications and one presented a TBX1 gene deletion. Following NGS analysis, 12 patients presented pathogenic or likely pathogenic genetic variants, five of them, found in KAT6B, SHH, MYH11, MYH7 and EP300 genes, are novel. Using an algorithm that combines molecular techniques with clinical and genetic assessment, we determined the genetic contribution in 27.5% of the analyzed patients.
Transportational Cyclic Steps Created by Submarine Long-Runout Turbidity Currents
Authors: Wu Zhuyuan , Izumi Norihiro
Published: June 2022

In recent years, it has become a common understanding among researchers that one of the significant agents for forming a variety of submarine geomorphologies is turbidity currents that travel long distances, so-called long-runout turbidity currents. In this study, we present a mathematical model of the formation of cyclic steps due to long-runout turbidity currents based on the assumption that the highly concentrated layer near the bottom of the turbidity currents achieves an equilibrium state. The model uses the four governing equations: the momentum equation, the continuity equation, the diffusion/dispersion equation of suspended sediment, and the continuity equation of sediment on the bed (the Exner equation). We simplify the governing equations by ignoring the entrainment from ambient water since there is a distinct density interface above the highly concentrated lower layer of long-runout turbidity currents. We determine the dimensions of cyclic steps based on the solution. Agreement between the predictions and field observations turns out to be reasonable.
Medication Errors in Intensive Care Units: An Umbrella Review of Control Measures
Authors: Dionisi Sara , Giannetta Noemi , Liquori Gloria , De Leo Aurora , D’Inzeo Victoria , Orsi Giovanni Battista , Di Muzio Marco , Napoli Christian , Di Simone Emanuele
Published: June 2022

Medication errors are defined as “any preventable event that may cause or lead to inappropriate medication use or patient harm while the medication is in the control of the health care professional, patient, or consumer.” Such errors account for 30 to 50 percent of all errors in health care. The literature is replete with systematic reviews of medication errors, with a considerable number of studies focusing on systems and strategies to prevent errors in intensive care units, where these errors occur more frequently; however, to date, there appears to be no study that encapsulates and analyzes the various strategies. The aim of this study is to identify the main strategies and interventions for preventing medication errors in intensive care units through an umbrella review. The search was conducted on the following databases: PubMed, CINAHL, PsycInfo, Embase, and Scopus; it was completed in November 2020. Seven systematic reviews were included in this review, with a total of 47 studies selected. All reviews aimed to evaluate the effectiveness of a single intervention or a combination of interventions and strategies to prevent and reduce medication errors. Analysis of the results that emerged identified two macro-areas for the prevention of medication errors: systems and processes. In addition, the findings highlight the importance of adopting an integrated system of interventions in order to protect the system from harm and contain the negative consequences of errors.
Virtual Reality in Palliative Care: A Systematic Review
Authors: Martin Jessica L. , Saredakis Dimitrios , Hutchinson Amanda D. , Crawford Gregory B. , Loetscher Tobias
Published: June 2022

Background: Virtual reality (VR) using head-mounted displays (HMDs) has demonstrated to be an effective tool for treating various somatic and psychological symptoms. Technological advances and increased affordability of VR technology provide an interesting option for delivering psychological interventions to patients in palliative care. The primary aim of this systematic review was to synthesise the available research on the use of VR for enhancing psychological and somatic outcomes for palliative care patients. Secondary aims included assessing general satisfaction and overall usability. Method: A pre-registered systematic literature search was conducted according to PRISMA guidelines using OVID Emcare, Cochrane Library, Embase, Medline, PsycINFO, and PubMed Care Search: Palliative Care Knowledge Network. Peer-reviewed experimental, quasi-experimental, observational, case, and feasibility studies consisting of single or multiple VR sessions using HMDs that reported psychological and/or somatic outcomes were included. Results: Eight studies published between 2019 and 2021 were included, representing 138 patients. While the reported quantitative psychological and somatic outcomes were ambiguous, the qualitative outcomes were largely positive. Participants were generally satisfied with VR, and most studies reported the VR interventions as usable, feasible, and acceptable. Conclusions: VR shows promise in palliative care and generally addresses a range of symptoms with few adverse effects. Future research should consist of adequately powered RCTs evaluating dosage and focusing on providing meaningful activities to enhance outcomes further.
Exploring the Interactive Associations between Urban Built Environment Features and the Distribution of Offender Residences with a GeoDetector Model
Authors: Wan Tao , Shi Buhai
Published: June 2022

Offender residences have become a research focus in the crime literature. However, little attention has been paid to the interactive associations between built environment factors and the residential choices of offenders. Over the past three decades, there has been an unprecedented wave of migrant workers pouring into urban centers for employment in China. Most of them flowed into urban villages within megacities. Weak personnel stability and great mobility have led to the urban villages to be closely related to decreased public safety and the deterioration of social order. The YB district in China was selected as the study area, which is located in one of the most developed cities in Southern China and has an area of approximately 800 km2 and a population of approximately four million people. This study aims to explore the associations between the neighborhood environment and the offender residences by using the geographical detector model (GeoDetector) from the perspective of interaction. The conceptual framework is based on the social disorganization theory. The results found that urban villages were the most important variable with a relatively high explanatory power. In general, taking the urban village as the carrier, various places (hotels, entertainment places, and factories) within the urban village may be more likely to include offender residences. This study also found the social disorganization theory applicable in the non-Western context. These findings may have important implications for offender residences identification, crime prevention, and the management of urban villages in Chinese cities.
Hepatitis A Virus Infection and Molecular Research
Authors: Kanda Tatsuo , Sasaki-Tanaka Reina , Nakamoto Shingo
Published: June 2022

Hepatitis A virus (HAV) infection is a major cause of acute viral hepatitis globally, which can occasionally lead to acute liver failure (ALF) and acute-on-chronic liver failure (ACLF), which often result in death without liver transplantation [...]
Non-Classical HLA Determinants of the Clinical Response after Autologous Stem Cell Transplantation for Systemic Sclerosis
Authors: Boukouaci Wahid , Lansiaux Pauline , Lambert Nathalie C. , Picard Christophe , Clave Emmanuel , Cras Audrey , Marjanovic Zora , Farge Dominique , Tamouza Ryad
Published: June 2022

Systemic Sclerosis (SSc) is a chronic autoimmune disease with high morbidity and mortality. Autologous Hematopoietic Stem Cell Transplantation (AHSCT) is the best therapeutic option for rapidly progressive SSc, allowing increased survival with regression of skin and lung fibrosis. The immune determinants of the clinical response after AHSCT have yet to be well characterized. In particular, the pivotal role of the Human Leukocyte Antigen (HLA) system is not well understood, including the role of non-classical immuno-modulatory HLA-E and HLA-G molecules in developing tolerance and the role of Natural Killer cells (NK) in the immunomodulation processes. We retrospectively tested whether the genetic and/or circulating expression of the non-classical HLA-E and HLA-G loci, as well as the imputed classical HLA determinants of HLA-E expression, influence the observed clinical response to AHSCT at 12- and 24-month follow-up. In a phenotypically well-defined sample of 46 SSc patients classified as clinical responders or non-responders, we performed HLA genotyping using next-generation sequencing and circulating levels of HLA-G and quantified HLA-E soluble isoforms by ELISA. The -21HLA-B leader peptide dimorphism and the differential expression level of HLA-A and HLA-C alleles were imputed. We observed a strong trend towards better clinical response in HLA-E*01:03 or HLA-G 14bp Del allele carriers, which are known to be associated with high expression of the corresponding molecules. At 12-month post-AHSCT follow-up, higher circulating levels of soluble HLA-E were associated with higher values of modified Rodnan Skin Score (mRSS) (p = 0.0275), a proxy of disease severity. In the non-responder group, the majority of patients carried a double dose of the HLA-B Threonine leader peptide, suggesting a non-efficient inhibitory effect of the HLA-E molecules. We did not find any correlation between the soluble HLA-G levels and the observed clinical response after AHSCT. High imputed expression levels of HLA-C alleles, reflecting more efficient NK cell inhibition, correlated with low values of the mRSS 3 months after AHSCT (p = 0.0087). This first pilot analysis of HLA-E and HLA-G immuno-modulatory molecules suggests that efficient inhibition of NK cells contributes to clinical response after AHSCT for SSc. Further studies are warranted in larger patient cohorts to confirm our results.
Can Cisplatin Therapy Be Improved? Pathways That Can Be Targeted
Authors: Ali Reem , Aouida Mustapha , Alhaj Sulaiman Abdallah , Madhusudan Srinivasan , Ramotar Dindial
Published: June 2022

Cisplatin (cis-diamminedichloroplatinum (II)) is the oldest known chemotherapeutic agent. Since the identification of its anti-tumour activity, it earned a remarkable place as a treatment of choice for several cancer types. It remains effective against testicular, bladder, lung, head and neck, ovarian, and other cancers. Cisplatin treatment triggers different cellular responses. However, it exerts its cytotoxic effects by generating inter-strand and intra-strand crosslinks in DNA. Tumour cells often develop tolerance mechanisms by effectively repairing cisplatin-induced DNA lesions or tolerate the damage by adopting translesion DNA synthesis. Cisplatin-associated nephrotoxicity is also a huge challenge for effective therapy. Several preclinical and clinical studies attempted to understand the major limitations associated with cisplatin therapy, and so far, there is no definitive solution. As such, a more comprehensive molecular and genetic profiling of patients is needed to identify those individuals that can benefit from platinum therapy. Additionally, the treatment regimen can be improved by combining cisplatin with certain molecular targeted therapies to achieve a balance between tumour toxicity and tolerance mechanisms. In this review, we discuss the importance of various biological processes that contribute to the resistance of cisplatin and its derivatives. We aim to highlight the processes that can be modulated to suppress cisplatin resistance and provide an insight into the role of uptake transporters in enhancing drug efficacy.
Targeted Modulation of Interferon Response-Related Genes with IFN-Alpha/Lambda Inhibition
Authors: Sur Debpali , Leonova Katerina , Levi Bar , Markowitz Shany Ivon , Cohen-Harazi Raichel , Gitlin Ilya , Gurova Katerina , Gudkov Andrei , Pinhasov Albert , Koman Igor , Nesher Elimelech
Published: June 2022

Interferon (IFN) signaling resulting from external or internal inflammatory processes initiates the rapid release of cytokines and chemokines to target viral or bacterial invasion, as well as cancer and other diseases. Prolonged exposure to IFNs, or the overexpression of other cytokines, leads to immune exhaustion, enhancing inflammation and leading to the persistence of infection and promotion of disease. Hence, to control and stabilize an excessive immune response, approaches for the management of inflammation are required. The potential use of peptides as anti-inflammatory agents has been previously demonstrated. Our team discovered, and previously published, a 9-amino-acid cyclic peptide named ALOS4 which exhibits anti-cancer properties in vivo and in vitro. We suggested that the anti-cancer effect of ALOS4 arises from interaction with the immune system, possibly through the modulation of inflammatory processes. Here, we show that treatment with ALOS4 decreases basal cytokine levels in mice with chronic inflammation and prolongs the lifespan of mice with acute systemic inflammation induced by irradiation. We also show that pretreatment with ALOS4 reduces the expression of IFN alpha, IFN lambda, and selected interferon-response genes triggered by polyinosinic-polycytidylic acid (Poly I:C), a synthetic analog of viral double-stranded RNA, while upregulating the expression of other genes with antiviral activity. Hence, we conclude that ALOS4 does not prevent IFN signaling, but rather supports the antiviral response by upregulating the expression of interferon-response genes in an interferon-independent manner.
Solo vs. Chorus: Monomers and Oligomers of Arrestin Proteins
Authors: Gurevich Vsevolod , Gurevich Eugenia
Published: June 2022

Three out of four subtypes of arrestin proteins expressed in mammals self-associate, each forming oligomers of a distinct kind. Monomers and oligomers have different subcellular localization and distinct biological functions. Here we summarize existing evidence regarding arrestin oligomerization and discuss specific functions of monomeric and oligomeric forms, although too few of the latter are known. The data on arrestins highlight biological importance of oligomerization of signaling proteins. Distinct modes of oligomerization might be an important contributing factor to the functional differences among highly homologous members of the arrestin protein family.
A Study of Zn-Ca Nanocomposites and Their Antibacterial Properties
Authors: Torres-Ramos M. I. , Martín-Camacho U. J. , González J. L. , Yañez-Acosta M. F. , Becerra-Solano L. , Gutiérrez-Mercado Y. K. , Macias-Carballo M. , Gómez Claudia M. , González-Vargas O. A. , Rivera-Mayorga J. A. , Pérez-Larios Alejandro
Published: June 2022

This study aimed to develop Ca2+ doped ZnO nanoparticles (NPs) and investigate their antibacterial properties against microorganisms of dental interest. Zn-Ca NPs were synthesized by the sol-gel method with different concentrations of Ca2+ (1, 3, and 5 wt. %) and subsequently characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), UV-vis spectroscopy and Fourier transform infrared spectroscopy (FT-IR). The Kirby–Bauer method was used to measure antibacterial effects. NPs showed the wurzite phase of ZnO and bandgap energies (Eg) from 2.99 to 3.04 eV. SEM analysis showed an average particle size of 80 to 160 nm. The treatments that presented the best antibacterial activity were Zn-Ca 3% and Zn-Ca 5%. ZnO NPs represent an alternative to generate and improve materials with antibacterial capacity for dental applications.
A Review of Social Media Website Users’ Interaction Paths with Governmental Accounts during the COVID-19 Pandemic
Authors: Badr Amal Mohammed Nabil Abdul Azim , Mohamed Tarek Ismail , Osman Nassereldin Abdel Qadir , Mikhaylov Alexey
Published: June 2022

This study aims to describe and analyze the paths of social media website users’ interaction with the content of government accounts during the COVID-19 pandemic. The objectives of the study are to describe the roles of government accounts as a tool to support the concept of health communication among users of social media websites and analyze the targeted and untargeted roles that have emerged in this context, describe the roles of government accounts as a tool of government communication and analyze how government institutions employed them during the COVID-19 pandemic, and describe the roles of social media websites as a tool to address the problems and challenges created by the social spacing procedures and solutions provided by these websites to deal with these challenges. This study surveys a sample of previous studies conducted on the roles of government accounts on social media websites during the COVID-19 pandemic. The tools of this study are both analytical and comparative as they analyze and compare the results of previous studies on the interaction paths of internet users. The results indicate that social media websites have been considered a pivotal tool for building a renewable and interactive system. This coincides with developments in the communication environment in which the individual is present within the social pattern, and its depth of impact has been demonstrated, particularly after the repercussions of the spread of the COVID-19 pandemic.
Analyzing the Use of Heuristics in a Virtual Reality Learning Context: A Literature Review
Authors: Mohammad Abdulghafour , Pedersen Line
Published: June 2022

With concepts such as immersion and presence, known as hedonistic qualities of importance in the virtual reality (VR) experience, the question arises whether the more pragmatic heuristics are effective in the evaluation of an artifact. However, despite the importance of the heuristics for artifact evaluations, the available studies do not provide a rigorous review of these heuristics and their efficiency. Thus, this review aims to look at how heuristics have been applied in various virtual learning environments (VLEs) that involve virtual reality learning activities, either for use in heuristic evaluations or as design principles. In addition, it examines how these heuristics support the evaluation of a more hedonistic quality, such as presence, and lastly, the aim is to gauge the estimated efficiency of heuristics as an evaluation method. This article is a systematic review of research investigating using heuristics in a virtual reality learning context. The review includes articles published from January 2017 to February 2022, and from the screened records, twelve articles were analyzed in full-text form. This review shows the versatility of heuristics and their applications as well as the key concepts that are vital to the user’s experience in a virtual reality learning context. This review indicates that heuristic evaluation is a valuable tool, as it provides a clear summary of what needs to be handled in the next iteration of the application.
Identification and Expression Analysis of Chemosensory Genes in the Antennal Transcriptome of Chrysanthemum Aphid Macrosiphoniella sanborni
Authors: Zhong Jian , Wang Yuxin , Lu Yufan , Ma Xiaoou , Zhang Qian , Wang Xiaoyue , Zhang Qixiang , Sun Ming
Published: June 2022

As one of the most destructive oligophagous pests, the chrysanthemum aphid (Macrosiphoniella sanborni) has seriously restricted the sustainable development of the chrysanthemum industry. Olfaction plays a critical role in the environmental perception of aphids, but very little is currently known about the chemosensory mechanism of M. sanborni. In this study, four MsanOBPs, four MsanCSPs, eight MsanORs, two MsanIRs and one MsanSNMP were identified among the 28,323 unigenes derived from the antennal transcriptome bioinformatic analysis of M. sanborni adults. Then, comprehensive phylogenetic analyses of these olfactory-related proteins in different aphid species were performed using multiple sequence alignment. Subsequently, the odor-specific and wing-specific expression profiles of these candidate chemosensory genes were investigated using quantitative real-time PCR. The data showed that most of these chemosensory genes exhibited higher expression levels in alate aphids. Among them, MsanOBP9, MsanOR2, MsanOR4, MsanOR43b-1, MsanCSP1, MsanCSP2, MsanCSP4, MsanIR25a and MsanIR40a in alate aphids showed remarkably higher expression levels than in apterous aphids under the effect of the host plant volatiles, indicating that these genes may take part in the specific behaviors of alate adults, such as host recognition, oviposition site selection and so on. This study lays the groundwork for future research into the molecular mechanism of olfactory recognition in M. sanborni.
Predictive Validity of the Postural Assessment Scale for Stroke (PASS) to Classify the Functionality in Stroke Patients: A Retrospective Study
Authors: Estrada-Barranco Cecilia , Sanz-Esteban Ismael , Giménez-Mestre Maria José , Cano-de-la-Cuerda Roberto , Molina-Rueda Francisco
Published: June 2022

The analysis of the predictive validity of a scale allows us to establish objectives in rehabilitation and to make decisions in the clinical setting. The objective of this study was to determine the validity of the Postural Assessment Scale for Stroke (PASS) to predict functionality at each stage of recovery in stroke patients. Methods: A retrospective study was carried out collecting data from patients admitted to a neurorehabilitation hospital. All patients having suffered a stroke less than two months before hospital admission were included in the study. The balance was measured with the PASS scale and the functionality with the Functional Independence Measure (FIM) scale. Simple linear regressions were performed to model the relationship between the PASS and FIM scores in the acute, subacute and chronic stages (6 and 12 months), as well as between the PASS scores at admission and the FIM values in the chronic stage. Results: The PASS scale showed a good predictive validity (R2 values from 0.54 to 0.87; β values from 1.99 to 2.62; p < 0.001) for FIM scores at acute, subacute and chronic stages, with lower goodness-of-fit for PASS scores at admission and FIM scores at 12 months (R2 = 0.383; β = 1.61 (0.96–2.26); p < 0.001). Cut-off points in the PASS scale to predict high functional level were 17.5 for the acute stage and 16.5 for the subacute and chronic stages. A score of 8.5 on the PASS scale measured in the acute phase predicted a high functional level at 12 months. Conclusion: The PASS scale is a useful tool to classify the functionality of stroke patients in the acute, subacute and chronic phases. The PASS score upon admission into the hospital can predict the functionality of the stroke patients after 12 months. However, future studies should be carried out to corroborate our findings with larger sample sizes.
Relationship between Lower Limbs Performance and Spinal Alignment in Parkinson’s Disease Patients: An Observational Study with Cross Sectional Design
Authors: Bissolotti Luciano , Rota Matteo , Calza Stefano , Sanchez Romero Eleuterio A. , Battaglino Andrea , Villafañe Jorge H.
Published: June 2022

Parkinson’s disease (PD) is a progressive neurodegenerative disease determining spinal deformities and muscle rigidity, weakness and dystonia that can be related to a change in muscular output during sit-to-stand tasks (STS). Purpose: The aim of this study was to determine the impacts of spinal alignment on lower limbs performance during STS tasks in Parkinson’s disease (PD) patients and healthy controls. Methods: In total, 43 consecutive PD patients (“PD” Group, 25 males and 18 females; age 73.7 ± 7.1) and 42 people not affected by any type of neurological disease (“CON” Group, 22 males, 20 females; age 69.8 ± 6.0) participated in the observational study. The clinical assessment included: IPAQ (International Physical Activity Questionnaire), Hoehn Yahr score, plumb-line distance from the spinous process of C7, kyphosis apex and the spinous process of L3 and S1. We used the Muscle Quality Index test (MQI) to assess muscle power output during STS in both groups. Results: The MQI test measurements of absolute and relative lower limb power was significantly lower in the PD group, in addition to a negative correlation with age and a positive correlation with PL-L3 in that group of patients. Conclusions: A final consideration regarding our results leads to the possibility that the preservation of lumbar lordosis may be one of the factors for maintaining efficient biomechanics of the lower limb muscles, with the preservation of the physiological contractile characteristics of these muscles being the objective for a multidisciplinary rehabilitation based on postural exercises of the spine and a program of training exercises for the lower limb muscles.
High First Trimester Levels of TSH as an Independent Risk Factor for Gestational Diabetes Mellitus: A Retrospective Cohort Study
Authors: Fernández Alba Juan Jesús , Castillo Lara María , Jiménez Heras José Manuel , Moreno Cortés Rocío , González Macías Carmen , Vilar Sánchez Ángel , San Laureano Florentino Carral , Moreno Corral Luis Javier
Published: June 2022

Although numerous articles have found an association between alterations in thyroid function and the risk of gestational diabetes mellitus (GDM), other studies have failed to demonstrate this association. This may be due to the different cut-off points used to define subclinical hypothyroidism. We aim to clarify the role of thyroid stimulating hormone (TSH) level in GDM within pregnant women with normal free thyroxine (fT4) levels. This retrospective cohort study was performed in 6775 pregnant women. The association between TSH and GDM was assessed by bivariate and multivariate logistic regression. Pregnant women with subclinical hypothyroidism are at significantly greater risk for GDM when compared with euthyroid pregnant women (OR = 1.85; 95% CI = 1.36–2.52). We have also observed that TSH levels increase the risk of GDM within euthyroid pregnant women, since the TSH levels between 2.5 and 4.71 showed a higher risk of GDM than those whose TSH levels are between 0.31 and 2.49 (OR = 1.54; 95% CI = 1.28–1.84). In addition, pregnant women with positive thyroid antibodies have almost 2.5 times the risk of developing GDM (OR = 2.47; 95% CI = 1.57–3.89). Our results support that in pregnant women with normal fT4 levels, higher first trimester TSH level implies a higher risk of GDM.
Influence of Electric Field on Proliferation Activity of Human Dermal Fibroblasts
Authors: Kamalov Almaz , Shishov Mikhail , Smirnova Natalia , Kodolova-Chukhontseva Vera , Dobrovol’skaya Irina , Kolbe Konstantin , Didenko Andrei , Ivan’kova Elena , Yudin Vladimir , Morganti Pierfrancesco
Published: June 2022

In this work, an electrically conductive composite based on thermoplastic polyimide and graphene was obtained and used as a bioelectrode for electrical stimulation of human dermal fibroblasts. The values of the electrical conductivity of the obtained composite films varied from 10−15 to 102 S/m with increasing graphene content (from 0 to 5.0 wt.%). The characteristics of ionic and electronic currents flowing through the matrix with the superposition of cyclic potentials ± 100 mV were studied. The high stability of the composite was established during prolonged cycling (130 h) in an electric field with a frequency of 0.016 Hz. It was established that the composite films based on polyimide and graphene have good biocompatibility and are not toxic to fibroblast cells. It was shown that preliminary electrical stimulation increases the proliferative activity of human dermal fibroblasts in comparison with intact cells. It is revealed that an electric field with a strength E = 0.02–0.04 V/m applied to the polyimide films containing 0.5–3.0 wt.% of the graphene nanoparticles activates cellular processes (adhesion, proliferation).
Species Identification and In Vitro Antifungal Susceptibility of Paecilomyces/Purpureocillium Species Isolated from Clinical Respiratory Samples: A Multicenter Study
Authors: Monpierre Lorra , Aït-Ammar Nawel , Valsecchi Isabel , Normand Anne-Cécile , Guitard Juliette , Riat Arnaud , Huguenin Antoine , Bonnal Christine , Sendid Boualem , Hasseine Lilia , Raberin Hélène , Dehais Marion , Ranque Stéphane , Hennequin Christophe , Piarroux Renaud , Dannaoui Eric , Botterel Françoise
Published: June 2022

Paecilomyces spp. are emerging fungal pathogens, where Paecilomyces lilacinus and Paecilomyces variotii are the most reported species. Taxonomic and phylogenetic revisions in this genus have shown that P. variotii represents a species complex, whereas P. lilacinus is related to another genus called Purpureocillium. The aims of this study were to identify clinical isolates of Paecilomyces spp. at the species level, and to determine their antifungal susceptibility profiles. 70 clinical Paecilomyces spp. isolates were identified by MALDI-TOF Mass Spectrometry (MS) and by multilocus rDNA genes sequencing including ITS and the D1/D2 genes. Among the 70 Paecilomyces spp. isolates, 28 were identified as P. lilacinum, 26 as P. variotii stricto sensu, and 16 as P. maximus. For antifungal susceptibility testing, Minimal Inhibitory Concentrations (MICs) or Minimal Effective Concentrations (MECs) were determined for 8 antifungals. All P. lilacinum isolates had high MICs and MECs of amphotericin B and echinocandins, respectively, unlike P. variotii and P. maximus. For azole drugs, MICs were molecule- and species- dependent. The differences in in vitro susceptibility to antifungals underline the importance of accurate species identification. The MALDI–TOF MS can be a good alternative in routine laboratory to ensure fast identification of Paecilomyces spp. and P. lilacinum.
Genomic and Chemical Profiling of B9, a Unique Penicillium Fungus Derived from Sponge
Authors: Chen Chaoyi , Qi Jiangfeng , He Yajing , Lu Yuanyuan , Wang Ying
Published: June 2022

This study presented the first insights into the genomic and chemical profiles of B9, a specific Penicillium strain derived from sponges of the South China Sea that demonstrated the closest morphological and phylogenetic affinity to P. paxillin. Via the Illumina MiSeq sequencing platform, the draft genome was sequenced, along with structural assembly and functional annotation. There were 34 biosynthetic gene clusters (BGCs) predicted against the antiSMASH database, but only 4 gene clusters could be allocated to known BGCs (≥50% identities). Meanwhile, the comparison between B9 and P. paxillin ATCC 10480 demonstrated clear distinctions in morphology, which might be ascribed to the unique environmental adaptability of marine endosymbionts. In addition, two novel pyridinones, penicidihydropyridone A (2) and penicidihydropyridone B (3), were isolated from cultures of B9, and structurally characterized by nuclear magnetic resonance (NMR) and mass spectrometry (MS). The absolute configurations were confirmed by comparison of experimental and calculated electronic circular dichroism (ECD) curves. In addition, structure-based molecular docking indicated that both neo-pyridinones might block the programmed cell death protein 1(PD-1) pathway by competitively binding a programmed cell death 1 ligand 1(PD-L1) dimer. This was verified by the significant inhibition rates of the PD-1/L1 interaction. These indicated that Penicillium sp. B9 possessed a potential source of active secondary metabolites.
The Impact of the Laterality on Radiographic Outcomes of the Bernese Periacetabular Osteotomy
Authors: Heimer Carsten Y. W. , Wu Chia H. , Perka Carsten , Hardt Sebastian , Göhler Friedemann , Bäcker Henrik C.
Published: June 2022

The purpose of this study was to compare the pre and postoperative radiographic findings and analyze the complication rate with respect to the laterality in periacetabular osteotomy in right-handed surgeons. Satisfaction rate and radiographic findings were prospectively collected between 2017 and 2019 and retrospectively reviewed. For analysis, all measurements of the CT scans were performed by a musculoskeletal fellowship-trained radiologist. Complications were classified into two categories: perioperative or postoperative. All surgeries were performed by three right-hand dominant hip surgeons. A total of 41 dysplastic hips (25 right and 16 left hips) in 33 patients were included. Postoperatively, a significantly lower acetabular index angle on the left side was observed at −2.6 ± 4.3 as compared to the right side at 1.6 ± 6.5 (p < 0.05). The change in Center edge (CE) angle was significantly lower for the left side 13.7 ± 5.5° than on the right side, measured at 18.4 ± 7.3 (p < 0.001); however, the overall CE angle was comparable at 38.5 ± 8.9° without any significant difference between the operated hips (left side at 37.8 ± 6.1° versus right side at 39.0 ± 10.3; p = 0.340). No significant differences in other radiographic measurements or surgical time were observed. For complications, the right side was more commonly affected, which may also explain a higher satisfaction rate in patients who were operated on the left hip with 92.3%. The change in lateral CE angle was significantly lower for the left side and the right hip seems to be predisposed to complications, which correlate with a lower satisfaction rate in right-handed surgeons.
An Integrated Approach for the Early Detection of Endometrial and Ovarian Cancers (Screenwide Study): Rationale, Study Design and Pilot Study
Authors: Peremiquel-Trillas Paula , Paytubi Sonia , Pelegrina Beatriz , Frias-Gomez Jon , Carmona Álvaro , Martínez José Manuel , de Francisco Javier , Benavente Yolanda , Barahona Marc , Briansó Ferran , Canet-Hermida Júlia , Caño Víctor , Vidal August , Zanca Alba , Baixeras Núria , Rodríguez Axel , Fernández-Gonzalez Sergi , Dueñas Núria , Càrdenas Laura , Aytés Álvaro , Bianchi Ilaria , Pavón Miquel Àngel , Reventós Jaume , Capellà Gabriel , Gómez David , Diaz Mireia , Ponce Jordi , Brunet Joan , Matias-Guiu Xavier , Bosch Francesc Xavier , de Sanjosé Silvia , Alemany Laia , Pineda Marta , Marin Fátima , Costas Laura
Published: June 2022

Screenwide is a case-control study (2017–2021) including women with incident endometrial and ovarian cancers (EC and OC), BRCA1/2 and MMR pathogenic variant carriers, and age-matched controls from three centers in Spain. Participants completed a personal interview on their sociodemographic factors, occupational exposure, medication, lifestyle, and medical history. We collected biological specimens, including blood samples, self-collected vaginal specimens, cervical pap-brush samples, uterine specimens, and, when available, tumor samples. The planned analyses included evaluation of the potential risk factors for EC/OC; evaluation of molecular biomarkers in minimally invasive samples; evaluation of the cost-effectiveness of molecular tests; and the generation of predictive scores to integrate different epidemiologic, clinical, and molecular factors. Overall, 182 EC, 69 OC, 98 BRCA pathogenic variant carriers, 104 MMR pathogenic variant carriers, and 385 controls were enrolled. The overall participation rate was 85.7%. The pilot study using 61 samples from nine EC cases and four controls showed that genetic variants at the variant allele fraction > 5% found in tumors (n = 61 variants across the nine tumors) were detected in paired endometrial aspirates, clinician-collected cervical samples, and vaginal self-samples with detection rates of 90% (55/61), 79% (48/61), and 72% (44/61) by duplex sequencing, respectively. Among the controls, only one somatic mutation was detected in a cervical sample. We enrolled more than 800 women to evaluate new early detection strategies. The preliminary data suggest that our methodological approach could be useful for the early detection of gynecological cancers.