bluuebunny/crossref_metadata_2025 huggingface dataset:
How Complex Developmental Trauma, Residential Out-of-Home Care and Contact with the Justice System Intersect
Authors: Bollinger Jenna , Scott-Smith Stephanie , Mendes Philip
Published: March 2017

Complex developmental trauma impacts on neurobiological development through the creation of a frightening and unpredictable environment in which the brain develops. This early experience results in an under-developed limbic system and pre-frontal cortex. For some children and young people, their experiences of early trauma lead them into the residential Out-of-Home Care (OOHC) system. Neurodevelopmental delays that occur as a result of early trauma and abuse often become particularly pronounced during adolescence, including limited impulse control, poor emotional regulation and attachment impairments. These same delays contribute to offending behaviour and subsequent contact with the justice system. Complex developmental trauma has serious repercussions both for the individual and the society in which he or she lives. These repercussions may take the form of offending behaviour and contact with the justice system, drug and alcohol abuse, and continuing cycles of abuse and violence within families or victimisation.
What Are Bucrania Doing in Tombs? Art and Agency in Neolithic Sardinia and Traditional South-East Asia
Authors: Robin Guillaume
Published: March 2017

The interior of Neolithic tombs in Europe is frequently decorated with carved and painted motifs. In Sardinia (Italy), 116 rock-cut tombs have their walls covered with bucrania (schematic depictions of cattle head and horns), which have long been interpreted as representations of a bull-like divinity. This article reviews similar examples of bucranium ‘art’ in the tombs of three traditional societies in South-East Asia, focusing on the agency of the motifs and their roles within social relationships between the living, the dead, and the spiritual world. From these ethnographic examples and the archaeological evidence in Sardinia, it is suggested that bucrania in Neolithic tombs were a specialized form of material culture that had multiple, cumulative effects and functions associated with social display, memory, reproduction, death, and protection.
Permeation fill-tube design for inertial confinement fusion target capsules
Authors: Rice B.S. , Ulreich J. , Fella C. , Crippen J. , Fitzsimmons P. , Nikroo A.
Published: March 2017

A unique approach for permeation filling of nonpermeable inertial confinement fusion target capsules with deuterium–tritium (DT) is presented. This process uses a permeable capsule coupled into the final target capsule with a 0.03-mm-diameter fill tube. Leak free permeation filling of glow-discharge polymerization (GDP) targets using this method have been successfully demonstrated, as well as ice layering of the target, yielding an inner ice surface roughness of 1-$\unicode[STIX]{x03BC}$m rms (root mean square). Finally, the measured DT ice-thickness profile for this experiment was used to validate a thermal model’s prediction of the same thickness profile.
A NEW DERIVATIVE-FREE CONJUGATE GRADIENT METHOD FOR LARGE-SCALE NONLINEAR SYSTEMS OF EQUATIONS
Authors: FANG XIAOWEI , NI QIN
Published: March 2017

We propose a new derivative-free conjugate gradient method for large-scale nonlinear systems of equations. The method combines the Rivaie–Mustafa–Ismail–Leong conjugate gradient method for unconstrained optimisation problems and a new nonmonotone line-search method. The global convergence of the proposed method is established under some mild assumptions. Numerical results using 104 test problems from the CUTEst test problem library show that the proposed method is promising.
Nucleic acids: function and potential for abiogenesis
Authors: Wachowius Falk , Attwater James , Holliger Philipp
Published: March 2017

The emergence of functional cooperation between the three main classes of biomolecules – nucleic acids, peptides and lipids – defines life at the molecular level. However, how such mutually interdependent molecular systems emerged from prebiotic chemistry remains a mystery. A key hypothesis, formulated by Crick, Orgel and Woese over 40 year ago, posits that early life must have been simpler. Specifically, it proposed that an early primordial biology lacked proteins and DNA but instead relied on RNA as the key biopolymer responsible not just for genetic information storage and propagation, but also for catalysis, i.e. metabolism. Indeed, there is compelling evidence for such an ‘RNA world’, notably in the structure of the ribosome as a likely molecular fossil from that time. Nevertheless, one might justifiably ask whether RNA alone would be up to the task. From a purely chemical perspective, RNA is a molecule of rather uniform composition with all four bases comprising organic heterocycles of similar size and comparable polarity and pKa values. Thus, RNA molecules cover a much narrower range of steric, electronic and physicochemical properties than, e.g. the 20 amino acid side-chains of proteins. Herein we will examine the functional potential of RNA (and other nucleic acids) with respect to self-replication, catalysis and assembly into simple protocellular entities.
Are stereotypes accurate? A perspective from the cognitive science of concepts
Authors: Bian Lin , Cimpian Andrei
Published: March 2017

In his 2012 book, Jussim suggests that people's beliefs about various groups (i.e., their stereotypes) are largely accurate. We unpack this claim using the distinction between generic and statistical beliefs – a distinction supported by extensive evidence in cognitive psychology, linguistics, and philosophy. Regardless of whether one understands stereotypes as generic or statistical beliefs about groups, skepticism remains about the rationality of social judgments.
Intelligence, competitive altruism, and “clever silliness” may underlie bias in academe
Authors: Madison Guy , Dutton Edward , Stern Charlotta
Published: March 2017

Why is social bias and its depressing effects on low-status or low-performing groups exaggerated? We show that the higher intelligence of academics has at best a very weak effect on reducing their bias, facilitates superficially justifying their biases, and may make them better at understanding the benefits of social conformity in general and competitive altruism specifically. We foresee a surge in research examining these mechanisms and recommend, meanwhile, reviving and better observing scientific ideals.
Choosing the right level of analysis: Stereotypes shape social reality via collective action
Authors: Tappin Ben M. , McKay Ryan T. , Abrams Dominic
Published: March 2017

In his 2012 book Jussim argues that the self-fulfilling prophecy and expectancy effects of descriptive stereotypes are not potent shapers of social reality. However, his conclusion that descriptive stereotypesper sedo not shape social reality is premature and overly reductionist. We review evidence that suggests descriptive stereotypes do have a substantial influence on social reality, by virtue of their influence on collective action.
Accuracy, bias, self-fulfilling prophecies, and scientific self-correction
Authors: Jussim Lee
Published: March 2017

In my Précis of Social Perception and Social Reality (Jussim 2012, henceforth abbreviated as SPSR), I argued that the social science scholarship on social perception and interpersonal expectancies was characterized by a tripartite pattern: (1) Errors, biases, and self-fulfilling prophecies in person perception were generally weak, fragile, and fleeting; (2) Social perceptions were often quite accurate; and (3) Conclusions appearing throughout the social psychology scientific literature routinely overstated the power and pervasiveness of expectancy effects, and ignored evidence of accuracy. Most commentators concurred with the validity of these conclusions. Two, however, strongly disagreed with the conclusion that the evidence consistently has shown that stereotypes are moderately to highly accurate. Several others, while agreeing with most of the specifics, also suggested that those arguments did not necessarily apply to contexts outside of those covered in SPSR. In this response, I consider all these aspects: the limitations to the tripartite pattern, the role of politics and confirmation biases in distorting scientific conclusions, common obstructions to effective scientific self-correction, and how to limit them.
Strong but flexible: How fundamental social motives support but sometimes also thwart favorable attractiveness biases
Authors: Agthe Maria , Maner Jon K.
Published: March 2017

Research corroborates the notion that fundamental social motives play an important role in biases that favor attractive people. Although an adaptationist framework expects favorable social effects of good looks in most situations and contexts, it simultaneously allows for potential negative social reactions and outcomes that may be elicited by physical attractiveness in other contexts. These effects of attractiveness reflect the reproductive opportunities and threats posed by potential mates and rivals.
Explanations for attractiveness-related positive biases in an evolutionary perspective of life history theory
Authors: Chen Bin-Bin
Published: March 2017

The mating-related evolutionary explanation that Maestripieri et al. offer does not apply to (1) infants' positive biases toward attractive individuals and (2) adults' positive biases toward attractive infants and children. They are best understood when integrated into an evolutionary life history framework. I argue that the life history of positive biases toward attractive individuals is driven by fundamental trade-offs made throughout development.
Understanding the physical attractiveness literature: Qualitative reviews versus meta-analysis
Authors: Feingold Alan
Published: March 2017

The target article is a qualitative review of selected findings in the physical attractiveness literature. This commentary explains why the meta-analytic approach, frequently used by other attractiveness reviewers, is preferable for drawing unbiased conclusions about the effects of attractiveness. The article's main contribution is affording a foundation for subsequent meta-analysis of the studies discussed in a subjective fashion.
Context matters for attractiveness bias
Authors: Lee Juwon , Adams Glenn , Li Yexin Jessica , Gillath Omri
Published: March 2017

To fully understand the attractiveness bias, we propose that contextual factors or affordances should be integrated into the mating-based evolutionary account of Maestripieri et al. We review examples highlighting the role of contextual factors in the perception of attractiveness and in attractiveness bias. These suggest contextual factors differentially afford the development of preference for attractive others into observed habits of mind.
Evolutionary explanations for financial and prosocial biases: Beyond mating motivation
Authors: Little Anthony C.
Published: March 2017

Mating motivation likely plays a role in bias to attractive individuals, but there are other complementary theories drawn from the evolutionary literature related to competition, friendship, and leadership selection that also make relevant predictions concerning biases towards attractive individuals. The relative balance of these factors will be context dependent and so help explain why the pattern of bias is sometimes variable.
The biasing effects of appearances go beyond physical attractiveness and mating motives
Authors: Olivola Christopher Y. , Todorov Alexander
Published: March 2017

The influence of appearances goes well beyond physical attractiveness and includes the surprisingly powerful impact of “face-ism” – the tendency to stereotype individuals based on their facial features. A growing body of research has revealed that these face-based social attributions bias the outcomes of labor markets and experimental economic games in ways that are hard to explain via evolutionary mating motives.
An assessment of the mating motive explanation of the beauty premium in market-based settings
Authors: Ravina Enrichetta
Published: March 2017

Labor market and real-life studies were not designed to discriminate between evolutionary and taste-based and stereotype explanations for the beauty premium, have too many confounding effects, and lack crucial information. Smaller-stake and experimental studies provide more compelling evidence in favor of mating motives and suggest the direction of future research for the economists' field studies.
Attentional and affective biases for attractive females emerge early in development
Authors: Rennels Jennifer Lynn , Verba Stephanie Ann
Published: March 2017

Predominant experience with females early in development results in infants developing an attractive, female-like facial representation that guides children's attention toward and affective preferences for attractive females. When combined with increased interest in the other sex at puberty, these early emerging biases might help explain the robust prosocial and financial biases men exhibit toward attractive women during adulthood.
Attractiveness bias: A cognitive explanation
Authors: Schein Stevie S. , Trujillo Logan T. , Langlois Judith H.
Published: March 2017

According to cognitive averaging theory, preferences for attractive faces result from their similarity to facial prototypes, the categorical central tendencies of a population of faces. Prototypical faces are processed more fluently, resulting in increased positive affect in the viewer.
From E-Group to Funerary Pyramid: Mortuary Cults and Ancestor Veneration in the Maya Centre of Nakum, Petén, Guatemala
Authors: Źrałka Jarosław , Koszkul Wiesław , Hermes Bernard , Velásquez Juan Luis , Matute Varinia , Pilarski Bogumił
Published: March 2017

Recent investigations at the Maya centre of Nakum (in Guatemala) enabled the study of the evolution of an interesting complex of buildings that started as the so-called E-Group, built during the Preclassic period (c. 600–300 bc). It was used for solar observations and rituals commemorating agricultural and calendrical cycles. During the Classic period (ad 250–800), the major building of the complex (Structure X) was converted into a large pyramidal temple where several burials, including at least one royal tomb, were placed. We were also able to document evidence of mortuary cults conducted by the Maya in the temple building situated above the burials. The architectural conversion documented in Structure X may reflect important religious and social changes: a transformation from the place where the Sun was observed and worshipped to the place where deceased and deified kings were apotheosized as the Sun Deity during the Classic. Thus the Maya transformed Structure X into one of the most sacred loci at Nakum by imbuing it with a complex solar and underworld symbolism and associating it with the cult of deified ancestors.
Minimum Codegree Threshold forC63-Factors in 3-Uniform Hypergraphs
Authors: GAO WEI , HAN JIE
Published: March 2017

LetC63be the 3-uniform hypergraph on {1, . . ., 6} with edges 123,345,561, which can be seen as the analogue of the triangle in 3-uniform hypergraphs. For sufficiently largendivisible by 6, we show that everyn-vertex 3-uniform hypergraphHwith minimum codegree at leastn/3 contains aC63-factor, that is, a spanning subhypergraph consisting of vertex-disjoint copies ofC63. The minimum codegree condition is best possible. This improves the asymptotic result obtained by Mycroft and answers a question of Rödl and Ruciński exactly.
Reduced fetal vitamin D status by maternal undernutrition during discrete gestational windows in sheep
Authors: Cleal J. K. , Hargreaves M. R. , Poore K. R. , Tang J. C. Y. , Fraser W. D. , Hanson M. A. , Green L. R.
Published: March 2017

Placental transport of vitamin D and other nutrients (e.g. amino acids, fats and glucose) to the fetus is sensitive to maternal and fetal nutritional cues. We studied the effect of maternal calorific restriction on fetal vitamin D status and the placental expression of genes for nutrient transport [aromatic T-type amino acid transporter-1 (TAT-1); triglyceride hydrolase/lipoprotein uptake facilitator lipoprotein lipase (LPL)] and vitamin D homeostasis [CYP27B1; vitamin D receptor (VDR)], and their association with markers of fetal cardiovascular function and skeletal muscle growth. Pregnant sheep received 100% total metabolizable energy (ME) requirements (control), 40% total ME requirements peri-implantation [PI40, 1–31 days of gestation (dGA)] or 50% total ME requirements in late gestation (L, 104–127 dGA). Fetal, but not maternal, plasma 25-hydroxy-vitamin D (25OHD) concentration was lower in PI40 and L maternal undernutrition groups (P<0.01) compared with the control group at 0.86 gestation. PI40 group placental CYP27B1 messenger RNA (mRNA) levels were increased (P<0.05) compared with the control group. Across all groups, higher fetal plasma 25OHD concentration was associated with higher skeletal muscle myofibre and capillary density (P<0.05). In the placenta, higher VDR mRNA levels were associated with higher TAT-1 (P<0.05) and LPL (P<0.01) mRNA levels. In the PI40 maternal undernutrition group only, reduced fetal plasma 25OHD concentration may be mediated in part by altered placental CYP27B1. The association between placental mRNA levels of VDR and nutrient transport genes suggests a way in which the placenta may integrate nutritional cues in the face of maternal dietary challenges and alter fetal physiology.
Honoring Earth and Space Scientists
Authors:   AGU
Published: March 2017

Remembering AGU members and others who have passed away.
Cellular preservation of musculoskeletal specializations in the Cretaceous bird Confuciusornis
Authors: Jiang Baoyu , Zhao Tao , Regnault Sophie , Edwards Nicholas P. , Kohn Simon C. , Li Zhiheng , Wogelius Roy A. , Benton Michael J. , Hutchinson John R.
Published: March 2017

The hindlimb of theropod dinosaurs changed appreciably in the lineage leading to extant birds, becoming more ‘crouched’ in association with changes to body shape and gait dynamics. This postural evolution included anatomical changes of the foot and ankle, altering the moment arms and control of the muscles that manipulated the tarsometatarsus and digits, but the timing of these changes is unknown. Here, we report cellular-level preservation of tendon- and cartilage-like tissues from the lower hindlimb of Early Cretaceous Confuciusornis. The digital flexor tendons passed through cartilages, cartilaginous cristae and ridges on the plantar side of the distal tibiotarsus and proximal tarsometatarsus, as in extant birds. In particular, fibrocartilaginous and cartilaginous structures on the plantar surface of the ankle joint of Confuciusornis may indicate a more crouched hindlimb posture. Recognition of these specialized soft tissues in Confuciusornis is enabled by our combination of imaging and chemical analyses applied to an exceptionally preserved fossil.
Constructive quantum interference in a bis-copper six-porphyrin nanoring
Authors: Richert Sabine , Cremers Jonathan , Kuprov Ilya , Peeks Martin D. , Anderson Harry L. , Timmel Christiane R.
Published: March 2017

The exchange interaction, J, between two spin centres is a convenient measure of through bond electronic communication. Here, we investigate quantum interference phenomena in a bis-copper six-porphyrin nanoring by electron paramagnetic resonance spectroscopy via measurement of the exchange coupling between the copper centres. Using an analytical expression accounting for both dipolar and exchange coupling to simulate the time traces obtained in a double electron electron resonance experiment, we demonstrate that J can be quantified to high precision even in the presence of significant through-space coupling. We show that the exchange coupling between two spin centres is increased by a factor of 4.5 in the ring structure with two parallel coupling paths as compared to an otherwise identical system with just one coupling path, which is a clear signature of constructive quantum interference.
Quantitative volumetric Raman imaging of three dimensional cell cultures
Authors: Kallepitis Charalambos , Bergholt Mads S. , Mazo Manuel M. , Leonardo Vincent , Skaalure Stacey C. , Maynard Stephanie A. , Stevens Molly M.
Published: March 2017

The ability to simultaneously image multiple biomolecules in biologically relevant three-dimensional (3D) cell culture environments would contribute greatly to the understanding of complex cellular mechanisms and cell–material interactions. Here, we present a computational framework for label-free quantitative volumetric Raman imaging (qVRI). We apply qVRI to a selection of biological systems: human pluripotent stem cells with their cardiac derivatives, monocytes and monocyte-derived macrophages in conventional cell culture systems and mesenchymal stem cells inside biomimetic hydrogels that supplied a 3D cell culture environment. We demonstrate visualization and quantification of fine details in cell shape, cytoplasm, nucleus, lipid bodies and cytoskeletal structures in 3D with unprecedented biomolecular specificity for vibrational microspectroscopy.
Pitfalls of exome sequencing: a case study of the attribution of HABP2 rs7080536 in familial non-medullary thyroid cancer
Authors: Gerhard Glenn S. , Bann Darrin V. , Broach James , Goldenberg David
Published: March 2017

Next-generation sequencing using exome capture is a common approach used for analysis of familial cancer syndromes. Despite the development of robust computational algorithms, the accrued experience of analyzing exome data sets and published guidelines, the analytical process remains an ad hoc series of important decisions and interpretations that require significant oversight. Processes and tools used for sequence data generation have matured and are standardized to a significant degree. For the remainder of the analytical pipeline, however, the results can be highly dependent on the choices made and careful review of results. We used primary exome sequence data, generously provided by the corresponding author, from a family with highly penetrant familial non-medullary thyroid cancer reported to be caused by HABP2 rs7080536 to review the importance of several key steps in the application of exome sequencing for discovery of new familial cancer genes. Differences in allele frequencies across populations, probabilities of familial segregation, functional impact predictions, corroborating biological support, and inconsistent replication studies can play major roles in influencing interpretation of results. In the case of HABP2 rs7080536 and familial non-medullary thyroid cancer, these factors led to the conclusion of an association that most data and our re-analysis fail to support, although larger studies from diverse populations will be needed to definitively determine its role.
Translational learning from clinical studies predicts drug pharmacokinetics across patient populations
Authors: Krauss Markus , Hofmann Ute , Schafmayer Clemens , Igel Svitlana , Schlender Jan , Mueller Christian , Brosch Mario , von Schoenfels Witigo , Erhart Wiebke , Schuppert Andreas , Block Michael , Schaeffeler Elke , Boehmer Gabriele , Goerlitz Linus , Hoecker Jan , Lippert Joerg , Kerb Reinhold , Hampe Jochen , Kuepfer Lars , Schwab Matthias
Published: March 2017

Early indication of late-stage failure of novel candidate drugs could be facilitated by continuous integration, assessment, and transfer of knowledge acquired along pharmaceutical development programs. We here present a translational systems pharmacology workflow that combines drug cocktail probing in a specifically designed clinical study, physiologically based pharmacokinetic modeling, and Bayesian statistics to identify and transfer (patho-)physiological and drug-specific knowledge across distinct patient populations. Our work builds on two clinical investigations, one with 103 healthy volunteers and one with 79 diseased patients from which we systematically derived physiological information from pharmacokinetic data for a reference probe drug (midazolam) at the single-patient level. Taking into account the acquired knowledge describing (patho-)physiological alterations in the patient cohort allowed the successful prediction of the population pharmacokinetics of a second, candidate probe drug (torsemide) in the patient population. In addition, we identified significant relations of the acquired physiological processes to patient metadata from liver biopsies. The presented prototypical systems pharmacology approach is a proof of concept for model-based translation across different stages of pharmaceutical development programs. Applied consistently, it has the potential to systematically improve predictivity of pharmacokinetic simulations by incorporating the results of clinical trials and translating them to subsequent studies.
Optimized Mass Spectrometry Analysis Workflow with Polarimetric Guidance for ex vivo and in situ Sampling of Biological Tissues
Authors: Woolman Michael , Gribble Adam , Bluemke Emma , Zou Jing , Ventura Manuela , Bernards Nicholas , Wu Megan , Ginsberg Howard J. , Das Sunit , Vitkin Alex , Zarrine-Afsar Arash
Published: March 2017

Spatially Targeted Mass Spectrometry (MS) analysis using survey scans with an imaging modality often requires consecutive tissue slices, because of the tissue damage during survey scan or due to incompatible sample preparation requirements between the survey modality and MS. We report two spatially targeted MS analysis workflows based on polarized light imaging guidance that use the same tissue sample for survey and targeted analysis. The first workflow is applicable for thin-slice analysis, and uses transmission-polarimetry-guided Desorption ElectroSpray Ionization Mass Spectrometry (DESI-MS), and confirmatory H&E histopathology analysis on the same slice; this is validated using quantitative digital pathology methods. The second workflow explores a polarimetry-guided MS platform for thick tissue assessment by developing reflection-mode polarimetric imaging coupled with a hand-held Picosecond InfraRed Laser (PIRL) MS ablation probe that requires minimal tissue removal to produce detectable signal. Tissue differentiation within 5–10 s of sampling with the hand-held probe is shown using multivariate statistical methods of the MS profiles. Both workflows were tasked with differentiating necrotic cancer sites from viable cancers using a breast tumour model, and their performance was evaluated. The use of the same tissue surface addresses mismatches in guidance due to intrinsic changes in tissue morphology over consecutive sections.
Porous Layered Double Hydroxides Synthesized using Oxygen Generated by Decomposition of Hydrogen Peroxide
Authors: Gonzalez Rodriguez P. , de Ruiter M. , Wijnands T. , ten Elshof J. E.
Published: March 2017

Porous magnesium-aluminium layered double hydroxides (LDH) were prepared through intercalation and decomposition of hydrogen peroxide (H2O2). This process generates oxygen gas nano-bubbles that pierce holes in the layered structure of the material by local pressure build-up. The decomposition of the peroxide can be triggered by microwave radiation or chemically by reaction with iodide (I−) ions. The carbonate LDH version [Mg0.80Al0.20(OH)2](CO3)0.1∙mH2O was synthesized by microwave-assisted urea coprecipitation and further modified by iodide or H2O2intercalation. High resolution Scanning Electron Microscopy (HR-SEM) and Brunauer-Emmet-Teller (BET) analysis were used to assess the morphology and surface area of the new porous materials. The presence of H2O2in the interlayer region and later decomposition triggered by microwave radiation generated more pores on the surface of the LDH platelets, increasing their specific surface area from initially 9 m2/g to a maximum of 67 m2/g. X-Ray Diffraction showed that the formation of the pores did not affect the remaining crystal structure, allowing possible further functionalization of the material.
Simulation and measurement of optimized microwave reflectivity for carbon nanotube absorber by controlling electromagnetic factors
Authors: Zhang Danfeng , Hao Zhifeng , Qian Yannan , Huang Yinxin , Bizeng  , Yang Zhenda , Qibai Wu
Published: March 2017

Heat-treatments may change the defect and surface organic groups of carbon nanotubes (CNTs), and lead to significant changes in the microwave electromagnetic parameter of CNTs. In this paper, the effect of heat-treatment time and temperature on the complex dielectric constant and permeability as well as the microwave reflectivity of CNTs was investigated. The experimental results indicated that the microwave absorption property of CNTs arises mainly from the high permittivity and consequent dielectric loss. Moreover, the heat-treatment resulted in increased dielectric constant of CNTs and significant improvement of the microwave absorption at frequency values of 2–18 GHz. The microwave reflectivity of CNT composites with a coating thickness of 3 mm was simulated by using the electromagnetic parameters. The absorption peak of CNTs treated at 700 °C had an amplitude of R = −48 dB, which occurred at 9 GHz. Below −10 dB, the composites treated at 900 °C had a bandwidth of 7 GHz. The position of the absorption peak concurred with the measured results. The results indicated that the microwave-absorption properties can be modified by adjusting heat-treatment temperature and time.
Exploring the Association Between Demographics, SLC30A8 Genotype, and Human Islet Content of Zinc, Cadmium, Copper, Iron, Manganese and Nickel
Authors: Wong Winifred P. , Allen Norrina B. , Meyers Matthew S. , Link Emma O. , Zhang Xiaomin , MacRenaris Keith W. , El Muayed Malek
Published: March 2017

A widely prevalent single nucleotide polymorphism, rs13266634 in the SLC30A8 gene encoding the zinc transporter ZnT8, is associated with an increased risk for T2DM. ZnT8 is mostly expressed in pancreatic insulin-producing islets of Langerhans. The effect of this variant on the divalent metal profile in human islets is unknown. Additionally, essential and non-essential divalent metal content of human islets under normal environmental exposure conditions has not been described. We therefore examined the correlation of zinc and other divalent metals in human islets with rs13266634 genotype and demographic characteristics. We found that the diabetes risk genotype C/C at rs13266634 is associated with higher islet Zn concentration (C/C genotype: 16792 ± 1607, n = 22, C/T genotype: 11221 ± 1245, n = 18 T/T genotype: 11543 ± 6054, n = 3, all values expressed as mean nmol/g protein ± standard error of the mean, p = 0.040 by ANOVA). A positive correlation between islet cadmium content and both age (p = 0.048, R2 = 0.09) and female gender (women: 36.88 ± 4.11 vs men: 21.22 ± 3.65 nmol/g protein, p = 0.007) was observed. Our results suggest that the T2DM risk allele C is associated with higher islet zinc levels and support prior evidence of cadmium’s higher bioavailability in women and its long tissue half-life.
Highly Permeable Graphene Oxide/Polyelectrolytes Hybrid Thin Films for Enhanced CO2/N2 Separation Performance
Authors: Heo Jiwoong , Choi Moonhyun , Chang Jungyun , Ji Dahye , Kang Sang Wook , Hong Jinkee
Published: March 2017

Separation of CO2 from other gasses offers environmental benefits since CO2 gas is the main contributor to global warming. Recently, graphene oxide (GO) based gas separation membranes are of interest due to their selective barrier properties. However, maintaining selectivity without sacrificing permeance is still challenging. Herein, we described the preparation and characterization of nanoscale GO membranes for CO2 separation with both high selectivity and permeance. The internal structure and thickness of the GO membranes were controlled by layer-by-layer (LbL) self-assembly. Polyelectrolyte layers are used as the supporting matrix and for facilitating CO2 transport. Enhanced gas separation was achieved by adjusting pH of the GO solutions and by varying the number of GO layers to provide a pathway for CO2 molecules. Separation performance strongly depends on the number of GO bilayers. The surfaces of the multilayered GO and polyelectrolyte films are characterized by atomic force microscopy and scanning electron microscopy. The (poly (diallyldimethylammonium chloride) (PDAC)/polystyrene sulfonate (PSS)) (GO/GO) multilayer membranes show a maximum CO2/N2 selectivity of 15.3 and a CO2 permeance of 1175.0 GPU. LbL-assembled GO membranes are shown to be effective candidates for CO2 separation based on their excellent CO2/N2 separation performance.
Insights from engraftable immunodeficient mouse models of hyperinsulinaemia
Authors: Maugham Michelle L. , Thomas Patrick B. , Crisp Gabrielle J. , Philp Lisa K. , Shah Esha T. , Herington Adrian C. , Chen Chen , Gregory Laura S. , Nelson Colleen C. , Seim Inge , Jeffery Penny L. , Chopin Lisa K.
Published: March 2017

Hyperinsulinaemia, obesity and dyslipidaemia are independent and collective risk factors for many cancers. Here, the long-term effects of a 23% Western high-fat diet (HFD) in two immunodeficient mouse strains (NOD/SCID and Rag1−/−) suitable for engraftment with human-derived tissue xenografts, and the effect of diet-induced hyperinsulinaemia on human prostate cancer cell line xenograft growth, were investigated. Rag1−/−and NOD/SCID HFD-fed mice demonstrated diet-induced impairments in glucose tolerance at 16 and 23 weeks post weaning. Rag1−/− mice developed significantly higher fasting insulin levels (2.16 ± 1.01 ng/ml, P = 0.01) and increased insulin resistance (6.70 ± 1.68 HOMA-IR, P = 0.01) compared to low-fat chow-fed mice (0.71 ± 0.12 ng/ml and 2.91 ± 0.42 HOMA-IR). This was not observed in the NOD/SCID strain. Hepatic steatosis was more extensive in Rag1−/− HFD-fed mice compared to NOD/SCID mice. Intramyocellular lipid storage was increased in Rag1−/− HFD-fed mice, but not in NOD/SCID mice. In Rag1−/− HFD-fed mice, LNCaP xenograft tumours grew more rapidly compared to low-fat chow-fed mice. This is the first characterisation of the metabolic effects of long-term Western HFD in two mouse strains suitable for xenograft studies. We conclude that Rag1−/− mice are an appropriate and novel xenograft model for studying the relationship between cancer and hyperinsulinaemia.
Pepper CaREL1, a ubiquitin E3 ligase, regulates drought tolerance via the ABA-signalling pathway
Authors: Lim Chae Woo , Park Chanmi , Kim Jung-Hyun , Joo Hyunhee , Hong Eunji , Lee Sung Chul
Published: March 2017

Drought stress conditions in soil or air hinder plant growth and development. Here, we report that the hot pepper (Capsicumannuum) RING type E3 Ligase 1 gene (CaREL1) is essential to the drought stress response. CaREL1 encodes a cytoplasmic- and nuclear-localized protein with E3 ligase activity. CaREL1 expression was induced by abscisic acid (ABA) and drought. CaREL1 contains a C3H2C3-type RING finger motif, which functions in ubiquitination of the target protein. We used CaREL1-silenced pepper plants and CaREL1-overexpressing (OX) transgenic Arabidopsis plants to evaluate the in vivo function of CaREL1 in response to drought stress and ABA treatment. CaREL1-silenced pepper plants displayed a drought-tolerant phenotype characterized by ABA hypersensitivity. In contrast, CaREL1-OX plants exhibited ABA hyposensitivity during the germination, seedling, and adult stages. In addition, plant growth was severely impaired under drought stress conditions, via a high level of transpirational water loss and decreased stomatal closure. Quantitative RT-PCR analyses revealed that ABA-related drought stress responsive genes were more weakly expressed in CaREL1-OX plants than in wild-type plants, indicating that CaREL1 functions in the drought stress response via the ABA-signalling pathway. Taken together, our results indicate that CaREL1 functions as a negative regulator of ABA-mediated drought stress tolerance.
A normative spatiotemporal MRI atlas of the fetal brain for automatic segmentation and analysis of early brain growth
Authors: Gholipour Ali , Rollins Caitlin K. , Velasco-Annis Clemente , Ouaalam Abdelhakim , Akhondi-Asl Alireza , Afacan Onur , Ortinau Cynthia M. , Clancy Sean , Limperopoulos Catherine , Yang Edward , Estroff Judy A. , Warfield Simon K.
Published: March 2017

Longitudinal characterization of early brain growthin-uterohas been limited by a number of challenges in fetal imaging, the rapid change in size, shape and volume of the developing brain, and the consequent lack of suitable algorithms for fetal brain image analysis. There is a need for an improved digital brain atlas of the spatiotemporal maturation of the fetal brain extending over the key developmental periods. We have developed an algorithm for construction of an unbiased four-dimensional atlas of the developing fetal brain by integrating symmetric diffeomorphic deformable registration in space with kernel regression in age. We applied this new algorithm to construct a spatiotemporal atlas from MRI of 81 normal fetuses scanned between 19 and 39 weeks of gestation and labeled the structures of the developing brain. We evaluated the use of this atlas and additional individual fetal brain MRI atlases for completely automatic multi-atlas segmentation of fetal brain MRI. The atlas is available online as a reference for anatomy and for registration and segmentation, to aid in connectivity analysis, and for groupwise and longitudinal analysis of early brain growth.
Dopant size effects on novel functionalities: High-temperature interfacial superconductivity
Authors: Suyolcu Y. Eren , Wang Yi , Baiutti Federico , Al-Temimy Ameer , Gregori Giuliano , Cristiani Georg , Sigle Wilfried , Maier Joachim , van Aken Peter A. , Logvenov Gennady
Published: March 2017

Among the range of complex interactions, especially at the interfaces of epitaxial oxide systems, contributing to the occurrence of intriguing effects, a predominant role is played by the local structural parameters. In this study, oxide molecular beam epitaxy grown lanthanum cuprate-based bilayers (consisting of a metallic (M) and an insulating phase (I)), in which high-temperature superconductivity arises as a consequence of interface effects, are considered. With the aim of assessing the role of the dopant size on local crystal structure and chemistry, and on the interface functionalities, different dopants (Ca2+, Sr2+ and, Ba2+) are employed in the M-phase, and the M–I bilayers are investigated by complementary techniques, including spherical-aberration-corrected scanning transmission electron microscopy. A series of exciting outcomes are found: (i) the average out-of-plane lattice parameter of the bilayers is linearly dependent on the dopant ion size, (ii) each dopant redistributes at the interface with a characteristic diffusion length, and (iii) the superconductivity properties are highly dependent on the dopant of choice. Hence, this study highlights the profound impact of the dopant size and related interface chemistry on the functionalities of superconducting oxide systems.
Efficient ECG Compression and QRS Detection for E-Health Applications
Authors: Elgendi Mohamed , Mohamed Amr , Ward Rabab
Published: March 2017

Current medical screening and diagnostic procedures have shifted toward recording longer electrocardiogram (ECG) signals, which have traditionally been processed on personal computers (PCs) with high-speed multi-core processors and efficient memory processing. Battery-driven devices are now more commonly used for the same purpose and thus exploring highly efficient, low-power alternatives for local ECG signal collection and processing is essential for efficient and convenient clinical use. Several ECG compression methods have been reported in the current literature with limited discussion on the performance of the compressed and the reconstructed ECG signals in terms of the QRS complex detection accuracy. This paper proposes and evaluates different compression methods based not only on the compression ratio (CR) and percentage root-mean-square difference (PRD), but also based on the accuracy of QRS detection. In this paper, we have developed a lossy method (Methods III) and compared them to the most current lossless and lossy ECG compression methods (Method I and Method II, respectively). The proposed lossy compression method (Method III) achieves CR of 4.5×, PRD of 0.53, as well as an overall sensitivity of 99.78% and positive predictivity of 99.92% are achieved (when coupled with an existing QRS detection algorithm) on the MIT-BIH Arrhythmia database and an overall sensitivity of 99.90% and positive predictivity of 99.84% on the QT database.
Bats pre-adapt sensory acquisition according to target distance prior to takeoff even in the presence of closer background objects
Authors: Amichai Eran , Yovel Yossi
Published: March 2017

Animals execute sensorimotor sequences to optimize performance of complex actions series. However, the sensory aspects of these sequences and their dynamic control are often poorly understood. We trained bats to fly to targets at different distances, and analysed their sensory behavior before and during flight to test whether they assess target distance before flight and how they adapt sensory acquisition in different situations. We demonstrate that bats’ sensory acquisition during approach-flight is more flexible than previously described. We identified acoustic parameters that illustrate that bats assess target distance before takeoff. We show that bats adapt their echolocation approach-sequences to target distance - ignoring closer background objects. At shorter distances, bats initiated their echolocation approach-sequence with distance-appropriate parameters, thus entering the approach sensory sequence “in step”. Our results suggest that in order to perform fine flight-manoeuvres, bats must maintain their sensorimotor plan in phase. To do this, they adapt acquisition according to target distance before initiating a complex sensory sequence based on a sensorimotor feedback-loop, even in complex acoustic environments, which impose other sensory reactions and restrictions. Though studying this in non-echolocating animals may prove difficult, such mechanisms are probably widely used in nature whenever complex series of sensorimotor actions are required.
Novel contactless approach for assessment of venous occlusion plethysmography by video recordings at the green illumination
Authors: Kamshilin Alexei A. , Zaytsev Valeriy V. , Mamontov Oleg V.
Published: March 2017

Occlusion plethysmography is an important method for assessment of the status of the cardiovascular system, which provides valuable information concerning arterial and venous blood flow, including mechanisms of their regulation. All up-to-date systems estimate change of the limb’s volume during occlusion by contact-type sensors. The objective of the research is demonstration of feasibility of the novel approach to measuring the blood flow during venous occlusion by using imaging photoplethysmography (PPG). Twenty healthy individuals participated in the experiment. We used four synchronized video cameras to record all-around view of the forearm illuminated by the green light. After the recording, the PPG waveform was calculated in more than 4000 non-overlapping Regions of Interest (ROI). In the most of the ROIs, the waveform shape was typical for classical plethysmography with the distinctive linear growth of the signal. Speed of the signal change was the same along the forearm but it varied along the forearm’s circumference. These findings allows us to hypothesize that the PPG waveform is the direct consequence of the forearm blood flow. Therefore, the novel technique could be applied to the same medical examinations as the classical plethysmography, but it is more advantageous because of non-contact nature and easiness in implementation.
PKCα diffusion and translocation are independent of an intact cytoskeleton
Authors: Hui Xin , Sauer Benjamin , Kaestner Lars , Kruse Karsten , Lipp Peter
Published: March 2017

Translocation of cytosolic cPKC to the plasma membrane is a key event in their activation process but its exact nature is still unclear with particular dispute whether sole diffusion or additional active transport along the cell’s cytoskeleton contributes to cPKC’s dynamics. This was addressed by analyzing the recruitment behavior of PKCα while manipulating the cytoskeleton. Photolytic Ca2+ uncaging allowed us to quantify the kinetics of PKCα redistribution to the plasma membrane when fused to monomeric, dimeric and tetrameric fluorescence proteins. Results indicated that translocation kinetics were modulated by the state of oligomerization as expected for varying Stokes’ radii of the participating proteins. Following depolymerization of the microtubules and the actin filaments we found that Ca2+ induced membrane accumulation of PKCα was independent of the filamentous state of the cytoskeleton. Fusion of PKCα to the photo-convertible fluorescent protein Dendra2 enabled the investigation of PKCα-cytoskeleton interactions under resting conditions. Redistribution following spatially restricted photoconversion showed that the mobility of the fusion protein was independent of the state of the cytoskeleton. Our data demonstrated that in living cells neither actin filaments nor microtubules contribute to PKCα’s cytosolic mobility or Ca2+-induced translocation to the plasma membrane. Instead translocation is a solely diffusion-driven process.
Periaqueductal Grey differential modulation of Nucleus Accumbens and Basolateral Amygdala plasticity under controllable and uncontrollable stress
Authors: Horovitz Omer , Richter-Levin Alon , Xu Lin , Jing Liang , Richter-Levin Gal
Published: March 2017

Resilience has been conceptualized in part as a dynamic process that includes the ability to adapt to stressful conditions. As such it encompasses the extent to which neural plasticity may be promoted. The current study examined metaplasticity by referring to the “plasticity of synaptic plasticity” in a neural circuit composed of the basolateral amygdala (BLA) and the nucleus accumbens (NAcc), using behavioural stress controllability with or without preceding stimulation of the dorsal periaqueductal gray (i.e. dPAG priming). A tendency for increased plasticity in the controllable versus the uncontrollable group was found in both the BLA and NAcc. dPAG priming suppressed NAcc LTP in all groups, but it suppressed BLA LTP only in the uncontrollable group, demonstrating dissociation between either controllable and uncontrollable groups or the NAcc and BLA. Thus, metaplasticity in the dPAG-BLA-NAcc circuit regulated differentially by controllable or uncontrollable stress may underlie stress coping, and thus contribute to stress-related psychopathologies.
Social cognition in neuropsychiatric populations: a comparison of theory of mind in schizophrenia and mesial temporal lobe epilepsy
Authors: Okruszek Łukasz , Bala Aleksandra , Wordecha Małgorzata , Jarkiewicz Michał , Wysokiński Adam , Szczepocka Ewa , Piejka Aleksandra , Zaborowska Oliwia , Szantroch Marta , Rysz Andrzej , Marchel Andrzej
Published: March 2017

Social cognition deficits are observed both in patients with schizophrenia (SCZ) and in patients with mesial temporal lobe epilepsy (MTLE). This may be due to dysfunction of the amygdala network, which is a common feature of both diseases. In this study, SCZ (n = 48) or MTLE (n = 31) and healthy controls (HC, n = 47) completed assessments of mentalising (Reading Mind in the Eyes Test, RMET) and basic cognitive processing, e.g., working memory, executive functions and psychomotor speed (Trail-Making Test B and Digit Symbol). SCZ were also assessed with the Positive And Negative Syndrome Scale (PANSS). We found that the RMET scores of the two clinical groups were similar (p > 0.05) and lower than in the HCs (SCZ: p < 0.05; MTLE: p < 0.001). In the next step, SCZ were split into two groups with respect to the level of symptoms. Analysis of the RMET scores revealed no differences between the HC (M = 25.7 ± 4.1) and POS-LO (M = 25.3 ± 4.8); both groups outperformed the POS-HI group (M = 21.3 ± 5.2) and the MTLE group (M = 20.8 ± 4.6). No differences were found for the median-split with regard to negative symptoms. In SCZ, the mind-reading deficit appears to be associated with the level of positive symptoms. Both POS-HI and MTLE patients present significant mentalising deficits compared to healthy controls.