bluuebunny/crossref_metadata_2025 huggingface dataset:
Pengembangan Handout Biologi dengan Media Autoplay Materi Sistem Ekskresi pada Manusia
Authors: Yenyen Oktavia , Prayitno Trio Ageng
Published: December 2022

Education 4.0 is a response to the needs of the industrial revolution 4.0 where humans and technology are adapted to generate new opportunities more creatively and innovatively. Teachers are required to master skills, the ability to adapt to new technologies and the challenges of the world, this aims to increase interest in learning and students' motivation to learn so they don't feel bored in the learning process. This study aims to determine the development of Biology Handouts with Media Autoplay material on the Excretory System in humans that is valid and feasible to use using the R&D (Research and Development) research method and the 4D Thiagarajan model. This research is limited to only three stages, namely Define, Design and Develop and ends with the stages of media expert validation and material expert validation. In research and development of biology handouts with autoplay media, the excretory system material in humans is very valid or feasible to use. The feasibility of the biology handout with autoplay media was obtained from the results of the media expert's validation of 97.5% and the material expert's validation results of 97.3%.
Pengembangan Media Pembelajaran Pop Up Book pada Materi Bangun Ruang
Authors: Abner Dean , Avianty Donna , Sefaverdiana Putri Vina
Published: December 2022

The study aims to produce pop up book media on spatial materials which aims to determine the responses of students and teachers and to determine the quality and effectiveness of the media developed. This media development process used a 4D development model. The 4D development model consists of 4 main stages, namely: Define, Design, Develop and Disseminate. However, in this research, it is limited only to the develop stage. This research was conducted at SD Kristen Brawijaya 1 Malang grade 6 with a sample of 6 students. This study produces an average expert validation of 3.8 this value is included in the very good criteria. The results of the research student responses get an average value of 89.2% with very good criteria. Student learning outcomes after using this media get an average value of 86.7 or above the student's completeness score, which is ≥ 75.
Hubungan Antara Kekuatan Otot Lengan dan Ketrampilan Servis Bawah pada Peserta Ekstrakurikuler Bolavoli SMP Negeri 3 Dullah
Authors: Renngur Saadia , Saputo Yulianto Dwi , Lufthansa Luthfie
Published: December 2022

This research was conducted in SMP Negeri 3 Dullah, Tual City with the focus of the research on the relationship between arm muscle strength and lower service skills of extracurricular participants. The purpose of the study was to determine the relationship between arm muscle strength and lower serve skills in volleyball extracurricular participants. Techniques for determining skills tests, and determining samples for extracurricular participants were 20 people. By using the correlational method. Correlation research is a research that involves collecting data to determine whether there is a relationship and the degree of relationship between two or more variables.Based on the analysis of research data from 20 participants, it is known that there is a relationship between arm muscle strength and service skills in volleyball extracurricular participants at SMP Negeri 3 Dullah, Tual City, the magnitude of the relationship can be seen from the correlation between arm muscle strength and bottom service skills. volley that is equal to 1.03. The conclusion is supported by the results of the t-test, namely Fcount > Ftable the value of Fcount is 2.16 and Ftable 0,190 then 2.16 > 0,190, this means that the hypothesis H1 is accepted and H0 is rejected. Then there is a significant positive correlation or relationship between arm muscle strength and lower service skills in volleyball extracurricular participants at SMP Negeri 3 Dullah, Tual City. accepted at the 85% confidence level.
Pengembangan Booklet Pengolahan Limbah Industri Tahu Menggunakan SSF-Wetlands dengan Tanaman Eceng Gondok
Authors: Sofiyullah Ahmad Nur Holis , Munawwaroh Anita
Published: December 2022

Liquid waste generated from the tofu industry contains organic substances, namely 40%-60% protein, 25%-50% carbohydrates, 10% fat, and other suspended solids that can undergo physical, chemical, and biological changes to produce toxic substances. To overcome the pollution caused by the disposal of industrial liquid waste, research on the development of a tofu industrial waste treatment booklet using subsurface flow-wetlands was carried out with water hyacinth plants. This aims to improve the understanding and skills of tofu industry owners so that tofu industry owners can practice on their own about tofu industrial waste treatment after reading the booklet. This research method uses the Thiagarajan media development model, there are 4 stages, namely: definition, design, development, and disseminate. However, this research was carried out only until the development stage. The results of the validation assessment validation test from material experts obtained an assessment percentage of 86% and media experts obtained an assessment percentage of 82.85% with the achievement criteria being very suitable or very valid according to the BSNP criteria. In conclusion, the booklet developed has very interesting qualifications, is very appropriate, and is effective, and has few revisions. so that the booklet media is suitable to be used and disseminated as a media for outreach to the owners of the tofu industry.
Peran Tindakan Sosial Masyarakat dalam Ritual Pembagunan Rumah Suku di Desa Dubesi Kecamatan Nanaet Dubesi Kabupaten Belu Nusa Tenggara Timur
Authors: Mali Roswitha Eleonora , Setiani Puspita Pebri , Novariyanto Rizky Agung
Published: December 2022

This study aims to determine the role of community social action in the ritual of building tribal houses and the goals and benefits contained in the ritual of building tribal houses. Role is the position or status of a person in social life who carries out rights and obligations. In the ritual of building a tribal house, each has its own role, especially in the process of carrying out the ritual of building a tribal house. Social action is an individual action that is directed to others and has meaning both for oneself and for others.

The method used in this research is descriptive analysis method. This method is used to be able to understand and provide a clear picture of the problems related to the content of this research. This study uses a qualitative approach because the researcher considers the problems studied are quite complex and dynamic so that the data obtained from the resource persons are collected using a natural method, namely direct interviews with the resource persons so that they get natural answers.

The results of this study indicate that the process of carrying out the ritual of building a tribal house follows four rules that must be carried out, namely: haleka hanematan, Tuda Fos, Hamutuk Ibun hamutuk Lian, and Sona Fahi. And also in the ritual process of implementing the construction of tribal houses, it is divided into four stages, namely the preparation stage, the implementation stage, the inauguration stage and the closing stage. The results of the study also show that the one who plays an important role in the ritual of building a tribal house is the malun because the malun is a person who comes from the descendants of the male ancestor of the founder of the tribal house that is being built.
Makna Kore Metan Masyarakat Imigran Timor-Timor
Authors: Colo Florentina Fatima , Setiani Puspita Pebri
Published: December 2022

This study aims to obtain an objective description of the meaning of kore methane in the East Timorese immigrant community in the village of Biboki Anleu. The meaning of kore methane is ancestral wealth that is passed down from generation to generation until today. The meaning contained in the Korean Metan traditional ceremony is that this tradition is still being carried out and is a sign that this tradition still has a certain function or meaning.Methodologically this research uses descriptive analysis method. Descriptive analysis is a research method that describes all data or the state of the subject or object of research then analyzed and compared based on the current reality. This research is a research that uses a qualitative approach. Qualitative research aims to understand, look for the meaning behind the data, to find the truth, both empirical, sensual and logical truths. Based on the data obtained through interviews, this type of research is descriptive qualitative research. Qualitative descriptive is research that produces descriptive data in the form of written or spoken words from people and observed behavior from phenomena that occur. Qualitative methods are methods that tend to be associated with the subjective nature of a social reality. The results of this study note that the meaning of kore methane for the immigrant communities of Timor-Timor is a tradition that is still carried out by the immigrant communities of Timor-Timor to clarify ethnicity, race, and culture. This is proof that the kore methane ceremony has its own function for the immigrant community of Timor-Timor. The conclusion is that the kore methane ceremony starts from the planning, preparation and closing stages. This tradition has a meaning or sign of love for people who have died.
Analisis Analisis Masalah Pembelajaran dan Literasi Teknologi Siswa SMA Laboratorium UM Malang sebagai Dasar Pengembangan Media
Authors: Ramadhani Amelia , Setiawan Deny , Mahanal Susriyati , Priambodo Bagus , Amalia Enik
Published: December 2022

Technological literacy is very necessary in a learning, one of which is biology learning. This is because biology is an abstract material, so media that can visualize it are needed with the help of technological literacy. The purpose of this research was to describe students' difficulties in biology material, biology learning methods, and students' understanding of technological literacy. The research method used is a survey method. Respondents in this research, namely biology teachers and students of class XII MIPA with a total of 39 respondents. Data collection techniques used consisted of interviews, questionnaires, and observations. The data obtained were analyzed using descriptive analysis. The results of the research revealed that the material considered the most difficult by students was KD 3.2 for bioprocessed material in cells with a percentage of 28.2%. This is mostly caused by the lack of supporting media (46.2%) and as many as 60% of students stated that teachers only use the lecture method in learning biology. In addition, the data obtained on the average level of students' technological literacy understanding of 83.08%. The results of this study are expected to be used as preliminary data to develop learning media containing technological literacy.
Giant Isolated Left Hydrosalpinx Simulating Ovarian Cyst in a Sexually Inactive Adolescent Successfully Treated with Laparoscopy
Authors:   Department of Pediatric Surgery, Postgraduate Institute of Child Health & Research and KT Children Government University Teaching Hospital Rajkot 360005 Gujarat India., Anilkumar Trambadia Rajvi , Mfonobong Anthony Favour ,   Department of Pediatric Surgery, Postgraduate Institute of Child Health & Research and KT Children Government University Teaching Hospital Rajkot 360005 Gujarat India., Ashit Chhaniara Rohan ,   Department of Pediatric Surgery, Postgraduate Institute of Child Health & Research and KT Children Government University Teaching Hospital Rajkot 360005 Gujarat India., R. Govani Dhaval ,   Department of Pediatric Surgery, Postgraduate Institute of Child Health & Research and KT Children Government University Teaching Hospital Rajkot 360005 Gujarat India., Cavalcante Correia Rafael ,   Children’s Hospital of the Sao Jose do Rio Preto Medical School, Santa Casa de Tatui General Hospital, 330 Maneco Pereira Road, Tatui 18273000, Sao Paulo, Brazil., V. Patel Ramnik ,   Department of Pediatric Surgery, Postgraduate Institute of Child Health & Research and KT Children Government University Teaching Hospital Rajkot 360005 Gujarat India.
Published: December 2022

Most cases of symptomatic hydrosalpinx requiring surgical treatment are caused by sexually transmitted diseases in adult population where the patient is sexually active.
Microcontroller Based Device for Capacitive Biosensor Readout
Authors:   School of Electrical and Computer Engineering, National Technical University of Athens, Athens 15780, Greece., A Vasilopoulou , A Georgas ,   School of Electrical and Computer Engineering, National Technical University of Athens, Athens 15780, Greece., E Hristoforou ,   School of Electrical and Computer Engineering, National Technical University of Athens, Athens 15780, Greece.
Published: December 2022

Nowadays, problems in healthcare sector seem to be more crucial than ever. In a bid to reduce the number of deaths due to several diseases by taking precautions, science has turned to the development of biosensors able to offer early diagnosis.
Effects of Vitamin D Supplementation on Exacerbation in Patients with Severe COPD
Authors:   Institute for Occupational Health of R. North Macedonia–Skopje, Ss Cyril and Methodius University in Skopje, RN Macedonia, J Minov , S Stoleski ,   Institute for Occupational Health of R. North Macedonia–Skopje, Ss Cyril and Methodius University in Skopje, RN Macedonia, T Petrova ,   Department of Pharmacy Practice, Chicago State University, Chicago, USA, Kamcevska N Kocovska ,   General Hospital “8-mi septemvri” Skopje, Ss Cyril and Methodius University in Skopje, RN Macedonia, Ilievska D Buklioska , Janeva E Jovanovska ,   University Clinic for Pulmology and Allergology, Ss Cyril and Methodius University in Skopje, RN Macedonia, D Bislimovska ,   Institute for Occupational Health of R. North Macedonia–Skopje, Ss Cyril and Methodius University in Skopje, RN Macedonia, D Mijakoski ,   Institute for Occupational Health of R. North Macedonia–Skopje, Ss Cyril and Methodius University in Skopje, RN Macedonia
Published: December 2022

Introduction: Results from several studies indicated that vitamin D plays a role in a variety of immunologic processes such as modulation of inflammatory pathways and susceptibility to infections.
The Durable Lining: A New Tool for Malaria Vector Control Compared to Other Classical Methods (Long Lasting Insecticide Treated Nets “LLIN” During a Village Scale Long Term Trial in Angola. I. Entomological Evaluation.
Authors:   Director of Research, Le Majoral Building 34420 Portiragnes Plage, France (pjcarnevale2001@yahoo.fr), Carnevale Pierre , Toto Jean-Claude ,   OCEAC PO 288 Yaoundé Cameroon (jctotofr@yahoo.fr) (Vincent.foumane@yahoo.fr), Foumane Vincent ,   OCEAC PO 288 Yaoundé Cameroon (jctotofr@yahoo.fr) (Vincent.foumane@yahoo.fr), Fortes Filomeno ,   National Malaria Control Programme, Luanda, Angola (filomenofortes@gmail.com), Carnevale Guillaume ,   HSE, Batuan, Indonesia (gpcarnevale2001@yahoo.fr), Gay Frédérick ,   Sorbonne University, INSERM, Pierre Louis Institute of Epidemiology and Public Health, Pitié Salpétrière Hospital, APHP, F75013, Paris, France (frederick.gay@sorbonne–universite.fr).
Published: December 2022

Background: Deltamethrin (“δ”) treated durable lining (“DL”) or “insecticide treated plastic sheeting” (“δITPS”) were used for a long term village–scale malaria vector control (VC) program in 8 villages around Balombo town
Does the Booster Vaccination Provide Advantages to Prevent Reinfection Over 1 or 2 Doses of COVID–19 Vaccine?
Authors:   Specialist in Family and Community Medicine, Health Center Santa Maria de Benquerencia. Regional Health Service of Castilla la Mancha (SESCAM), Toledo, Spain., Luis Turabian Jose
Published: December 2022

Background: COVID–19 booster effectiveness against reinfections is not clearly known.
Incidence Rates and Risk Factors for Covid-19 Booster Adverse Reactions in General Medicine
Authors:   Specialist in Family and Community Medicine Health Center Santa Maria de Benquerencia. Regional Health Service of Castilla la Mancha (SESCAM), Toledo, Spain., Luis Turabian Jose
Published: December 2022

Background: Data on the incidence rate and risk factors of covid-19 booster adverse reactions are critical to success vaccination programs.
Acute Kidney Injury in the Critically Ill Still Remains a Challenge
Authors: Janssen van Doorn Karin
Published: December 2022

Acute kidney injury (AKI) is a common complication of critical illness and is associated with high morbidity and mortality. The epidemiology and pathogenesis of AKI and changes in renal function and preventive strategies are areas of interest. Although the aetiology of AKI is often multifactorial, sepsis has been consistently found to be a leading contributing factor in AKI during critical illness. Despite revised guidelines and better haemodynamic management, the outcome of AKI is still a reason for concern. Critically ill patients with AKI have significantly improved short-time prognosis with current treatment standards but are more prone to develop increased morbidity in the near future.
New Biocompatible Haemodiafiltration Membrane to Enable Maximum Substitution for Sensitive Patients
Authors: Tricker Jane
Published: December 2022

In many European countries, high-volume online haemodiafiltration (OL-HDF) is becoming the method of choice for treating patients with chronic kidney disease. The high convective (Qs >20 L/session) and diffusive properties of this treatment have been shown to be beneficial for patient survival. For optimum outcomes, the dialyser membrane must be able to cope with high transmembrane pressures. For this reason, the most widely-used membranes for this technique are synthetic and asymmetric in structure, making it easier for the membrane to divert the pressure away from its surface. However, patients allergic or sensitive to synthetic molecules, cannot access these high convective volumes (CV) reached in high-volume HDF, because alternative semi-natural membranes for allergic patients, such as cellulose acetate-based membranes, do not have adequate pressure-handling properties for high-volume HDF.

At this symposium, a new type of cellulose triacetate (CTA)-based membrane that is biocompatible, able to perform high-volume OL-HDF, and suitable for sensitive patients was introduced.
Analisis Quality In Use Model ISO/IEC 25010 Pada Penggunaan Aplikasi TikTok
Authors: Umroh Masfufahtul , Kusuma Wardani Syafrina Dyah , Rani Aulia Cahya , Riski Achmad Maulana , Bimantara M. Dwi Cahya , Rolliawati Dwi
Published: December 2022

Teknologi yang semakin berkembang saat ini tentu saja membuat pengguna internet terutama di Indonesia semakin banyak. Pengguna internet paling sering mengakses konten hiburan salah satunya pada aplikasi TikTok. Aplikasi TikTok menjadi aplikasi yang populer di kalangan para remaja terutama saat pandemi Covid 19 ini. Banyak sekali masyarakat terutama publik figur yang menggunakan aplikasi tersebut untuk bisnis atau hanya membuat konten yang kreatif dan bermanfaat bagi pengguna lain. Dalam penelitian ini, peneliti ingin melakukan analisis aplikasi TikTok yang semakin hari pengguna aplikasi tersebut semakin banyak berdasarkan model ISO IEC 25010 dengan kategori Quality In Use. Karakteristik dari Quality In Use yang akan dianalisis antara lain karakteristik Effectiveness, Efficiency, Satisfaction, Freedom from risk, dan Context coverage. Metode penelitian yang digunakan adalah kuantitatif deskriptif. Hasil yang didapatkan dari penelitian ini adalah bahwa pada karakteristik Effectivenes dan karakteristik Satisfaction memiliki rata-rata yang lebih tinggi dibandingkan dengan beberapa karakteristik lainnya yaitu sebesar 84%. Dari hasil tersebut, dapat disimpulkan bahwa aplikasi TikTok ini dapat memenuhi kebutuhan pengguna mulai dari mencari informasi sampai mendapatkan informasi yang diinginkan dan bernilai akurat.
Assessing adults with neuroatypical conditions suspected of having dementia
Authors: Janicki Matthew
Published: December 2022

Dementia is increasing as nations’ populations across the world age and older adults survive in increasing number. Dementia has various etiologies and forms of expression thus accurate diagnosis is always a challenge. Detection of any adult-age cognitive impairment in health care settings is difficult in general but can be especially challenging among adults with neuroatypical or neurodivergent conditions (NACs). Most clinical guidelines/protocols applicable to the general population for assessing mild cognitive impairment or dementia do not include considerations when assessing adults with NACs. This article addresses the obstacles to early detection and assessment of adults with NACs and recounts what one national group undertook to raise awareness of this obstacle. These conditions often present assessment challenges as adults with NACs often have problems with comprehension, oral communication, motor task performance, recognition of assessment related visuals, and comfort in testing situations. Clinicians assessing adults with NACs face challenges due to an inappropriateness of using standardized dementia assessment measures, are often untrained or unfamiliar with discerning pre-existing conditions from new cognitive impairment and are uninformed as to how to adapt the testing situation. The investigation into NACs and dementia assessment lead to a series of recommendations to raise awareness among clinicians, seek to enlist professional organizations in adapting existing instruments, and increase research into NACs and dementia.
Vegetable Waste Biodrying Treatment for Energy Recovery as Refuse Derived Fuel Potential
Authors: Septiariva Iva Yenis , Suryawan I Wayan Koko , Sari Mega Mutiara
Published: December 2022

Vegetable waste is a type of biodegradable organic waste found in every location in Indonesia. In addition, vegetable waste also dominates food waste. One of the ways to use vegetable waste is to use energy recovery. Energy recovery of vegetable waste can be done by time-dependent biodrying and bioactivator. This study aimed to determine the effect of time and activator application on the vegetable waste biodrying process. In this study, 0.5 kg of waste is used with an airflow rate of 15 liters/minute, the temperature in the process is in the range of 28.4-34.1°C. The bioactivators used in this study were baker's yeast, tempeh, and tape. The maximum decrease in mass occurs in the biodrying process with the addition of a bioactivator. The multivariate effect test results showed an effect of time and bioactivator on changes in water content and caloric value. However, the interaction between time and bioactivator only affects the water content. This is because the degradation process occurs utilizing microorganisms stored in the bioactivator liquid and water in vegetable waste. Further research is needed to know the effect of other variables in the biodrying process, especially the right detention time and bioactivators that accelerate the rate of degradation.
Techno-Economic Analysis of Extractive Butanol Fermentation by Immobilized Cells with Large Extractant Volume
Authors: Darmayanti Rizki Fitria , Muharja, Maktum , Zhao Tao , Gao Ming , Tashiro Yukihiro , Sakai Kenji , Sonomoto Kenji
Published: December 2022

There are several challenges for ABE fermentation to be used in an industrial scale including the low of butanol yield, the high energy requirement for separation and purification, and the competeness of sugar with food demand as substrat. In this study, techno-economical aspects of ABE fermentation by using immobilized cells with large extractant volume were studied. Overall production process was designed using rice straw as raw material which is semi-hydrolyzed to produce cellobiose, glucose, xylose, and arabinose mixture. Concentrated sugar was then fed to extractive fed-batch fermentation using immobilized cells. Finally, extractant was recovered and products were purified by distillation column. By evaluating this process design for the small scale capacity of 238 kg-butanol and acetone/day, the energy requirement was 41.3 MJ/kg-butanol and acetone and the cost was 1.91 $/kg-butanol and acetone. Although the cost was higher than butanol produced by petrochemical process of 1.08 $/kg-butanol, it may reduce if the scale is increased.
The Influence of Price and Product Quality on Purchase Decisions on Thrift Clothes at Shabira Store, Tulang Bawang Regency
Authors: Safitri Malik Annisa , Jayanti Afrida , F Sanjaya Vicky
Published: December 2022

The purpose of this study was to determine the effect of price and product quality on purchasing decisions. The subject used in this study is a picture of a secondhand shop from the Shabira Store, Tulang Bawang Regency. The method used is a questionnaire instrument, the sample used is consumers at the Shabira Store, Tulang Bawang Regency, totaling 66 respondents. The statistical test used is PLS-based Structural Equation Modeling. Validity test using Factor Loading then Reliability Test using Cronbach'c Alpha, Composite Reliability and Average Variance Extracted (AVE) values. Thus, all the results of the index items used in this study are valid and reliable, thus
Crop rotation increases root biomass and promotes the correlation of soil dissolved carbon with the microbial community in the rhizosphere
Authors: Chen Shuaimin , Yao Fanyun , Mi Guohua , Wang Lichun , Wu Haiyan , Wang Yongjun
Published: December 2022

As essential approaches for conservation agricultural practices, straw residue retention and crop rotation have been widely used in the Mollisols of Northeast China. Soil organic carbon, root development and microbial community are important indicators representing soil, crop and microbiota, respectively, and these factors work together to influence soil fertility and crop productivity. Studying their changes and interactions under different conservation practices is crucial to provide a theoretical basis for developing rational agricultural practices. The experiment in this study was conducted using the conventional practice (continuous maize without straw retention, C) and three conservation practices, namely, continuous maize with straw mulching (CS), maize–peanut rotation (R), and maize–peanut rotation with straw mulching (RS). Straw mulching (CS) significantly increased soil total organic carbon (TOC), active organic carbon (AOC), and microbial biomass carbon (MBC), but did not promote maize yield. Maize–peanut rotation (R and RS) significantly increased dissolved organic carbon (DOC) in the rhizosphere by promoting root growth, and maize yield (increased by 10.2%). For the microbial community structure, PERMANOVA and PCoA indicated that the bacterial community differed significantly between rhizosphere soil and bulk soil, but the fungal community shifted more under different agricultural practices. The correlation analysis indicated that the rotation system promoted the association between the soil DOC and the microbial community (especially the bacterial community), and straw mulching enhanced the connection between the soil TOC and the fungal community. Some plant growth–promoting rhizobacteria (includingBacillus,Streptomyces,Rhizobium, andPseudomonas) were enriched in the rhizosphere soil and were increased in the rotation system (R and RS), which might be due to an increase in the soil rhizosphere DOC level. These beneficial microbes had significantly negative correlations with several fungal groups (such asMycosphaerella,Penicillium,ParaphomaandTorula) that were classified as plant pathotrophs by FUNGuild. These results indicated that ensuring plant root development and improving root–bacteria interactions are of great importance to guarantee crop yield when implementing conservation tillage practices.
Illegal wildlife trade in two special economic zones in Laos: Underground–open-sale fluctuations in the Golden Triangle borderlands
Authors: van Uhm Daan P. , Zhang Mingxia
Published: December 2022

The Laos borders with China, Myanmar, and Thailand have been identified as vulnerable hubs for illegal wildlife trade. In particular, some special economic zones (SEZs) in Laos are linked to illegal wildlife products, including tiger bones, rhino horn, and ivory for sale. SEZs are zones granted more free market-oriented economic policies and flexible governmental measures. In this study, we conducted on-site observations to identify high-valued wildlife, including (parts of) tigers, rhinos, bears, and pangolins in 2 of the 13 SEZs—the Golden Triangle and Boten SEZs—and conducted semistructured interviews with anonymous participants in 2017 and 2019. The trend regarding illegal wildlife trade in these SEZs seems to fluctuate. In the Golden Triangle SEZ, we found that the illegal trade in wildlife is present but occurs more covertly than previously observed; the trade transformed underground to online social media. In Boten SEZ, we found a decrease in bear bile products and an increase in the volume of tiger products openly for sale. Informants explained that the decrease of openly sold wildlife in the Golden Triangle SEZ has been influenced by media and political attention as well as inspections from local authorities, while in Boten SEZ, illegal wildlife traders diversified into tiger products, due to the decline in bear bile products and the reduction in the opportunity to obtain them.
Contrast-induced acute kidney injury and its contemporary prevention
Authors: Sůva Marek , Kala Petr , Poloczek Martin , Kaňovský Jan , Štípal Roman , Radvan Martin , Hlasensky Jiří , Hudec Martin , Brázdil Vojtěch , Řehořová Jitka
Published: December 2022

The complexity and application range of interventional and diagnostic procedures using contrast media (CM) have recently increased. This allows more patients to undergo procedures that involve CM administration. However, the intrinsic CM toxicity leads to the risk of contrast-induced acute kidney injury (CI-AKI). At present, effective therapy of CI-AKI is rather limited. Effective prevention of CI-AKI therefore becomes crucially important. This review presents an in-depth discussion of CI-AKI incidence, pathogenesis, risk prediction, current preventive strategies, and novel treatment possibilities. The review also discusses the difference between CI-AKI incidence following intraarterial and intravenous CM administration. Factors contributing to the development of CI-AKI are considered in conjunction with the mechanism of acute kidney damage. The need for ultimate risk estimation and the prediction of CI-AKI is stressed. Possibilities of CI-AKI prevention is evaluated within the spectrum of existing preventive measures aimed at reducing kidney injury. In particular, the review discusses intravenous hydration regimes and pre-treatment with statins and N-acetylcysteine. The review further focuses on emerging alternative imaging technologies, alternative intravascular diagnostic and interventional procedures, and new methods for intravenous hydration guidance; it discusses the applicability of those techniques in complex procedures and their feasibility in current practise. We put emphasis on contemporary interventional cardiology imaging methods, with a brief discussion of CI-AKI in non-vascular and non-cardiologic imaging and interventional studies.
Data-independent acquisition proteomics reveals circulating biomarkers of coronary chronic total occlusion in humans
Authors: Li Jun , Jiang Xue-Jun , Wang Qun-Hui , Wu Xing-Liang , Qu Zhe , Song Tao , Wan Wei-Guo , Zheng Xiao-Xin , Yi Xin
Published: December 2022

Introduction

The pathophysiology of coronary chronic total occlusion (CTO) has not been fully elucidated.

Methods

In the present study, we aimed to investigate the potential plasma biomarkers associated with the pathophysiologic progression of CTO and identify protein dynamics in the plasma of CTO vessels immediately after successful revascularization. We quantitatively analyzed the plasma proteome profiles of controls (CON, n = 10) and patients with CTO pre- and post- percutaneous coronary intervention (PCI) (CTO, n = 10) by data-independent acquisition proteomics. We performed enzyme-linked immunosorbent assay (ELISA) to further confirm the common DEPs in the two-group comparisons (CON vs. CTO and CTO vs. CTO-PCI).

Results

A total of 1936 proteins with 69 differentially expressed proteins (DEPs) were detected in the plasma of patients with CTO through quantitative proteomics analysis. For all these DEPs, gene ontology (GO) analysis and protein-protein interaction (PPI) analysis were performed. The results showed that most of the proteins were related to the negative regulation of proteolysis, regulation of peptidase activity, negative regulation of hydrolase activity, humoral immune response, and lipid location. Furthermore, we identified 1927 proteins with 43 DEPs in the plasma of patients with CTO vessels after immediately successful revascularization compared to pre-PCI. GO analysis revealed that the above DEPs were enriched in the biological processes of extracellular structure organization, protein activation cascade, negative regulation of response to external stimulus, plasminogen activation, and fibrinolysis. More importantly, we generated a Venn diagram to identify the common DEPs in the two-group comparisons. Seven proteins, ADH4, CSF1, galectin, LPL, IGF2, IgH, and LGALS1, were found to be dynamically altered in plasma during the pathophysiological progression of CTO vessels and following successful revascularization, moreover, CSF1 and LGALS1 were validated via ELISA.

Conclusions

The results of this study reveal a dynamic pattern of the molecular response after CTO vessel immediate reperfusion, and identified seven proteins which would be the potential targets for novel therapeutic strategies to prevent coronary CTO.
Identification of PFKFB2 as a key gene for the transition from acute to old myocardial infarction in peripheral blood
Authors: Yang Xiangyu , Li Jie , Hu Xinyao , Zhang Yinzhuang , Kuang Yuanyuan , Liu Yubo , Liu Chenxi , Gao Haodong , Ma Li , Tang Jia , Ma Qilin
Published: December 2022

Objective

This study aims to analyze the gene expression profile of peripheral blood in different stages of myocardial infarction (MI) by transcriptome sequencing, and to study the gene expression characteristics of peripheral blood after MI.

Methods

Differentially expressed genes (DEGs) and weighted gene co-expression network analysis (WGCNA) were used to identify genes and modules associated with old myocardial infarction (OMI). Gene Ontology (GO) functional annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway annotation were applied to analyze the potential functions of genes. Hub genes were identified by Random Forest Classifier. CIBERSORT was used to provide an estimate of the abundance of 22 immune cells in peripheral blood. Quantitative polymerase chain reaction (qPCR) was used to detect gene expression levels in clinical samples. The cellular components (CC) of peripheral blood were counted by an automatic hematology analyzer.

Results

Through differential gene analysis and co-expression network analysis, 11 candidate genes were obtained. A random forest classifier identified 10 hub genes. Immune cell distribution of peripheral blood was found that T cell CD4 memory resting, NK cells resting, Dendritic cells activated, Mast cells resting, Monocytes and Neutrophils were correlated with OMI. Spearman correlation analysis found that PFKFB2 is related to the above immune cells. Low expression of PFKFB2 in peripheral blood of OMI was detected in clinical samples, and the relationship between PFKFB2 and peripheral blood immune cell counts was analyzed, which showed monocytes were associated with PFKFB2 in our study.

Conclusion

PFKFB2 was low expressed in OMI, and related to the distribution of immune cells. PFKFB2 may play a key role in reflecting the transition from AMI to OMI, and predicting the distribution of immune cells, which provided a new perspective for improving myocardial fibrosis and adverse remodeling.
Professionalization of science management—Comparing formal education and training across Germany, Poland, and Hungary
Authors: Müller Ursula , Toczyski Piotr , Regös Nora , Pliszczynska Olga , Jankovics Réka
Published: December 2022

Description of the examined questions

Our goal is to suggest a working definition of “science management” and to explore and systematically compare study programs related to this area. We focus on science management diplomas, short courses and workshops in three countries: Germany, Poland, and Hungary. What is common and what is different in the approaches to science management in three knowledge societies?

Methodology

Our method is descriptive and comparative. We use desk research to find out how the programs in science management are structured in three countries and what approaches are specific to them all.

Results

It became evident that in Germany there are plenty of offerings in terms of specialized study programs and short courses. There is a certain dynamic regarding the interconnectedness of research fields and the institutionalization of education and training. This leads to self-reinforcing dynamics concerning professionalization of science management, although science managers still can be hardly labeled as professionals. In Poland and Hungary there is still not much momentum to the process of professionalization, although first efforts are made. Programs such as European Research Area (ERA)-Fellowship might contribute to the evolution of multi-lateral networks between these countries and Germany. The awareness and broad understanding of science management might spread also in Central and Eastern European countries.

Conclusion

We conclude that the impact on the professionalization of science managers in Germany, Poland, and Hungary is unequal. Concerning training and education in Poland and Hungary, this article might be one step toward showing the broadness inherent to the concept of “science management.” It might stimulate more people in Central and Eastern European countries to think about this, to generate knew knowledge and to teach their insights within newly established study programs or short courses.
Impaired fasting glucose levels among perinatally HIV-infected adolescents and youths in Dar es Salaam, Tanzania
Authors: Nkinda Lilian , Buberwa Eliud , Memiah Peter , Ntagalinda Alieth , George Martin , Msafiri Frank , Joachim Agricola , Majigo Mtebe , Ramaiya Kaushik , Sunguya Bruno
Published: December 2022

Objective

This study assessed impaired fasting glucose and associated factors among perinatally HIV-infected adolescents and youths in Dar es salaam Tanzania.

Background

Impaired fasting glucose is a marker of heightened risk for developing type 2 diabetes among perinatally HIV-infected individuals. Therefore, identifying individuals at this stage is crucial to enable early intervention. Therefore, we assessed impaired fasting glucose (IFG) and associated factors among perinatally HIV-infected population in Dar es salaam Tanzania.

Methods

A cross-sectional study was conducted among 152 adolescents and youth attending HIV clinic at Muhimbili National Hospital and Infectious Disease Centre from July to August 2020. Fasting blood glucose (>8 hours) was measured using one-touch selects LifeScan, CA, USA. We also examined C-Reactive Protein and interleukin-6 inflammatory biomarkers in relation to impaired fasting glucose (IFG). Associations between categorical variables were explored using Chi-square, and poison regression with robust variance was used to calculate the prevalence ratios.

Results

Of the 152 participants, the majority were male (n=83[54.6%]), and the median age was 15(14-18) years. Overweight or obesity was prevalent in 16.4%, while more than one in ten (13.2%) had high blood pressure (≥149/90mmHg). All participants were on antiretroviral therapy (ART); 46% had used medication for over ten years, and about one in three had poor medication adherence. Among the recruited participants, 29% had impaired fasting glucose. The odds of IFG were two times higher in males compared to females (PR, 2.07, 95% CI 1.19 -3.59 p=0.001). Moreover, we found with every increase of Interleukin 6 biomarker there was a 1.01 probability increase of impaired fasting glucose (PR, 1.01, 95% CI 1.00 – 1.02 p=0.003).

Conclusion

About one in three perinatally HIV-infected youths had impaired fasting glucose in Dar es Salaam, Tanzania, with males bearing the biggest brunt. Moreover, with every increase of 1.101 of the probability of having IFG increased. This calls for urgent measures to interrupt the progression to diabetes disease and prevent the dual burden of disease for this uniquely challenged population.
Physical activity and risk of gallstone disease: A Mendelian randomization study
Authors: Qian Qilin , Jiang Han , Cai Bingyue , Chen Dingwan , Jiang Minmin
Published: December 2022

Objective: Given the association between physical activity and the reduced risk of gallstone disease as suggested in observational studies, a Mendelian randomization study was conducted to evaluate the causal nature of this association in genetic epidemiology.

Study: Including self-reported and accelerometer-based physical activity traits, the independent genetic variants associated with physical activity were selected from the corresponding genome-wide association studies as instrumental variables. The summary-level data for gallstone disease were sourced from the UK Biobank (7,682 cases and 455,251 non-cases) and FinnGen consortium (23,089 cases and 231,644 non-cases). Then, two-sample Mendelian randomization analysis was conducted. Inverse-variance weight (IVW), weighted median, and Mendelian randomization–Egger regression were determined through Mendelian randomization analyses. To ensure the robustness of the results, sensitivity analyses were also carried out in the study.

Results: The negative causality between the genetically predicted accelerometer-based “average acceleration” physical activity and the risk of gallstone disease was suggested in the UK Biobank study (p = 0.023, OR = 0.93, 95% CI: 0.87–0.99), and accelerometer-based “overall activity” physical activity and the risk of gallstone disease in the UK Biobank study (p = 0.017, OR = 0.38, 95% CI: 0.17–0.84). With accelerometer-based “average acceleration” physical activity negatively correlated with gallstone disease in the FinnGen consortium data (p = 0.001, OR = 0.94, 95% CI: 0.90–0.97). As for self-reported moderate-to-vigorous physical activity, however, there was no causality observed in both pieces of data.

Conclusion: Our studies provide the evidence suggesting a casual association between physical activities and gallstone disease through analysis of genetic data. As indicated by the research results, there is a possibility that a higher level of physical activities could mitigate the risk of gallstone disease.
Anti-PD-1 antibodies, a novel treatment option for advanced chemoresistant pulmonary lymphoepithelioma carcinoma
Authors: Zhou Na , Tang Hui , Yu Shuangni , Lin Yi , Wang Yingyi , Wang Yuzhou
Published: December 2022

Background

Pulmonary lymphoepithelioma-like carcinoma (LELC) exhibits a unique immune microenvironment, including high PD-L1 expression and abundant infiltrating-immune cells. However, the availability of PD-1/PD-L1 inhibitors in patients with LELC is still not determined.

Methods

A total of 36 cases of pulmonary LELC treated with PD-1/PD-L1 inhibitors were reviewed, including 10 cases from our institute and 26 cases included from the literature. The Kaplan-Meier method and log-rank test were utilized to analyze the survival outcomes of LELC patients receiving immunotherapy, and the factors related to immunotherapy response were further examined.

Results

Of the 10 patients from our institute, the median age was 53.5 years, adrenal glands and distant lymph nodes were the most common metastatic sites, and 4 of 8 (50%) patients had a PD-L1 TPS ≥50%. The median progression-free survival and overall survival in patients from our institute and from the literature were 11.6 and 27.3 months, 17.2 months and not reached, respectively. In all 36 patients, the objective response rate was as high as 57.6%. Patients with higher PD-L1 expression were more likely to have a tumor response, but the association of PD-L1 expression with survival time remains to be determined.

Conclusions

PD-1/PD-L1 inhibitors in patients with pulmonary LELC demonstrated a promising efficacy in retrospective cohorts, and deserve further validation in prospective studies administrating in front-line setting.
Frailty index based on laboratory tests improves prediction of short-and long-term mortality in patients with critical acute myocardial infarction
Authors: Bai Weimin , Hao Benchuan , Xu Lijun , Qin Ji , Xu Weihao , Qin Lijie
Published: December 2022

Background

Previous studies have shown that the frailty index based on laboratory tests (FI-Lab) can identify older adults at increased risk of adverse health outcomes. This study aimed to determine whether the FI-Lab is associated with mortality risk and can provide incremental improvements in risk stratification of patients with critical acute myocardial infarction (AMI).

Materials and methods

We conducted a secondary analysis of data from the Medical Information Mart for Intensive Care (MIMIC)-IV database. A 33-item FI-Lab was constructed. Outcomes of interest were in-hospital and 1-year mortality. Logistic regression models were used to investigate the association between the FI-Lab and outcomes. For the assessment of the incremental predictive value, the FI-Lab was added to several risk stratification scoring systems for critically ill patients, and the following indices were calculated: Δ C-statistic, integrated discrimination improvement (IDI), and net reclassification improvement (NRI).

Results

Out of 2,159 patients, 477 died in hospital (22.1%), and 898 died during the 1-year follow-up period. After adjustment for confounders, the FI-Lab was associated with increased in-hospital mortality [odds ratio (OR) = 1.06, 95% confidence interval (CI): 1.05–1.07] and 1-year mortality (OR = 1.05, 95% CI: 1.04–1.06) when assessed as a continuous variable (per 0.01-score increase). When assessed as a categorical variable, the FI-Lab was associated with in-hospital mortality (2nd Quartile: OR = 1.89, 95% CI: 1.18–3.03; 3rd Quartile: OR = 3.46, 95% CI: 2.20–5.46; and 4th Quartile: OR = 5.79, 95% CI: 3.61–9.28 compared to 1st Quartile) as well as 1-year mortality (2nd Quartile: OR = 1.66, 95% CI: 1.23–2.24; 3rd Quartile: OR = 2.40, 95% CI: 1.76–3.26; and 4th Quartile: OR = 3.76, 95% CI: 2.66–5.30 compared to 1st Quartile) after adjustment for confounders. The addition of the FI-Lab to all disease severity scores improved discrimination and significantly reclassified in-hospital and 1-year mortality risk.

Conclusion

The FI-Lab was a strong predictor of short- and long-term mortality in patients with critical AMI. The FI-Lab improved the ability to predict mortality in patients with critical AMI and therefore might be useful in the clinical decision-making process.