bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessment of spring cranioplasty biomechanics in sagittal craniosynostosis patients
Authors: Borghi Alessandro , Schievano Silvia , Rodriguez Florez Naiara , McNicholas Roisin , Rodgers Will , Ponniah Allan , James Greg , Hayward Richard , Dunaway David , Jeelani N. u. Owase
Published: August 2017

OBJECTIVE

Scaphocephaly secondary to sagittal craniosynostosis has been treated in recent years with spring-assisted cranioplasty, an innovative approach that leverages the use of metallic spring distractors to reshape the patient skull. In this study, a population of patients who had undergone spring cranioplasty for the correction of scaphocephaly at the Great Ormond Street Hospital for Children was retrospectively analyzed to systematically assess spring biomechanical performance and kinematics in relation to spring model, patient age, and outcomes over time.

METHODS

Data from 60 patients (49 males, mean age at surgery 5.2 ± 0.9 months) who had received 2 springs for the treatment of isolated sagittal craniosynostosis were analyzed. The opening distance of the springs at the time of insertion and removal was retrieved from the surgical notes and, during the implantation period, from planar radiographs obtained at 1 day postoperatively and at the 3-week follow-up. The force exerted by the spring to the patient skull at each time point was derived after mechanical testing of each spring model—3 devices with the same geometry but different wire thicknesses. Changes in the cephalic index between preoperatively and the 3-week follow-up were recorded.

RESULTS

Stiffer springs were implanted in older patients (p < 0.05) to achieve the same opening on-table as in younger patients, but this entailed significantly different—higher—forces exerted on the skull when combinations of stiffer springs were used (p < 0.001). After initial force differences between spring models, however, the devices all plateaued. Indeed, regardless of patient age or spring model, after 10 days from insertion, all the devices were open.

CONCLUSIONS

Results in this study provide biomechanical insights into spring-assisted cranioplasty and could help to improve spring design and follow-up strategy in the future.
Elaborate mapping of the posterior visual pathway in awake craniotomy
Authors: Shahar Tal , Korn Akiva , Barkay Gal , Biron Tali , Hadanny Amir , Gazit Tomer , Nossek Erez , Ekstein Margaret , Kesler Anat , Ram Zvi
Published: August 2017

OBJECTIVE

Resection of intraaxial tumors adjacent to the optic radiation (OR) may be associated with postoperative visual field (VF) deficits. Intraoperative navigation using MRI-based tractography and electrophysiological monitoring of the visual pathways may allow maximal resection while preserving visual function. In this study, the authors evaluated the value of visual pathway mapping in a series of patients undergoing awake craniotomy for tumor resection.

METHODS

A retrospective analysis of prospectively collected data was conducted in 18 patients who underwent an awake craniotomy for resection of intraaxial tumors involving or adjacent to the OR. Preoperative MRI-based tractography was used for intraoperative navigation, and intraoperative acquisition of 3D ultrasonography images was performed for real-time imaging and correction of brain shift. Goggles with light-emitting diodes were used as a standard visual stimulus. Direct cortical visual evoked potential (VEP) recording, subcortical recordings from the OR, and subcortical stimulation of the OR were used intraoperatively to assess visual function and proximity of the lesion to the OR. VFs were assessed pre- and postoperatively.

RESULTS

Baseline cortical VEP recordings were available for 14 patients (77.7%). No association was found between preoperative VF status and baseline presence of cortical VEPs (p = 0.27). Five of the 14 patients (35.7%) who underwent subcortical stimulation of the OR reported seeing phosphenes in the corresponding contralateral VF. There was a positive correlation (r = 0.899, p = 0.04) between the subcortical threshold stimulation intensity (3–11.5 mA) and the distance from the OR. Subcortical recordings from the OR demonstrated a typical VEP waveform in 10 of the 13 evaluated patients (76.9%). These waveforms were present only when recordings were obtained within 10 mm of the OR (p = 0.04). Seven patients (38.9%) had postoperative VF deterioration, and it was associated with a length of < 8 mm between the tumor and the OR (p = 0.05).

CONCLUSIONS

Intraoperative electrophysiological monitoring of the visual pathways is feasible but may be of limited value in preserving the functional integrity of the posterior visual pathways. Subcortical stimulation of the OR may identify the location of the OR when done in proximity to the pathways, but such proximity may be associated with increased risk of postoperative worsening of the VF deficit.
Impact of prenatal diagnosis of fetus-in-fetu
Authors: Sewell E.K. , Massa-Buck B. , Rubio E.I. , Massaro A.N. , Badillo A. , Puscasiu E. , Broth R. , Falika-King T. , Nies B. , Penn A.
Published: August 2017

Fetus in fetu (FIF) is an extremely rare condition (1/500,000 live births) in which a fetiform structure is incorporated into the body of its twin. FIF can be a diagnostic dilemma due to its similarity to a teratoma, but identification of FIF is important for subsequent medical and surgical management. We compare two cases of fetal masses diagnosed on prenatal imaging that were later identified as FIF through further radiological, surgical, and pathologic evaluation. We use these cases to illustrate key pre- and postnatal features of FIF and highlight the benefits of prenatal detection and follow-up for postnatal management.
Improving handover between the transport team and neonatal intensive care unit staff in neonatal transports using the plan-do-study-act tool
Authors: Kresch M.J. , Christensen S. , Kurtz M. , Lubin J.
Published: August 2017

OBJECTIVES:

The aim was to achieve 100% effective handover from the critical care transport team to the neonatal intensive care unit (NICU) medical team.

STUDY DESIGN:

All patients transferred from referring hospitals by the critical care transport team to the Level IV NICU were included. Data for each infant was collected prospectively. The percentage of transported patients for which medical team and nursing handover occurred was recorded. A quality improvement project was launched using the Plan-Do-Study-Act (PDSA) tool. We implemented several processes including call from the transport team before arrival and the completion of a transfer of care form on arrival to the NICU. The process measures and the outcome measure of completion of handover were monitored. Run charts of process measures and the outcome measure were analyzed.

RESULTS:

Completion of medical handover increased from 95% (baseline) to 100% after 3 PDSA cycles and this has been maintained for 18 consecutive months.

CONCLUSION:

Medical handover from the critical care transport team to the NICU medical staff has been achieved and sustained for all neonatal transports.
Troponin-T as a biomarker in neonates with perinatal asphyxia
Authors: Abiramalatha T. , Kumar M. , Chandran S. , Sudhakar Y. , Thenmozhi M. , Thomas N.
Published: August 2017

BACKGROUND:

Troponin-T is a commonly used cardiac biomarker, which could be useful in perinatal asphyxia. We aimed to analyze troponin-T concentrations in asphyxiated neonates and to correlate the concentrations with clinical outcomes.

METHODS:

Data were collected from electronic medical records of neonates diagnosed with perinatal asphyxia over a period of four years.

RESULTS:

There were 63 neonates with moderate to severe encephalopathy, in whom serial troponin-T concentrations had been done on days 1, 3, and 7. 53 (84%) asphyxiated infants had troponin-T concentration >100 pg/ml at 2–4 h of life. The difference in troponin-T concentrations between moderate and severe encephalopathy was not statistically significant (173 vs. 263 pg/ml, p value 0.40). The difference in the concentrations at 72 hours between cooled and non-cooled neonates was not significant (48.5 vs. 62.5 pg/ml, p value 0.22). Troponin-T concentration was significantly higher in babies with hypotensive shock and hepatic injury, but not acute kidney injury. There was no significant correlation between troponin-T and the extent of resuscitation needed. Troponin-T concentration on day 1 of life was significantly higher in babies who died than who survived (407 vs. 168 pg/ml, p value 0.03). ROC curve for troponin-T to predict mortality had an area under the curve (AUC) of 0.803; the best cut-off value (190 pg/ml) had 82% sensitivity and 80% specificity.

CONCLUSION:

There was no significant difference in troponin-T concentrations between cooled and non-cooled neonates. Troponin-T concentration had a good predictive accuracy for mortality before discharge.
Cerebral oxygenation and desaturations in preterm infants – a longitudinal data analysis
Authors: Mayer Benjamin , Pohl Moritz , Hummler Helmut D. , Schmid Manuel B.
Published: August 2017

OBJECTIVE:

Hypoxemic episodes commonly occur in very preterm infants and may be associated with several adverse effects. Cerebral tissue oxygen saturation (StO2) as measured by near infrared spectroscopy (NIRS) may be a useful measure to assess brain oxygenation. However, knowledge on variability of StO2 is limited in preterm infants at this time, so StO2 dependency on arterial oxygenation (SpO2) and heart rate (HR) was assessed in preterm infants using statistical methods of time series analysis.

STUDY DESIGN:

StO2, SpO2, and HR were recorded from 15 preterm infants every 2 seconds for six hours. Statistical methods of time series and longitudinal data analysis were applied to the data.

RESULT:

The mean StO2 level was found as 72% (95% confidence interval (CI) 55.5% –85.5%) based on a moving average process with a 5 minute order. Accordingly, longitudinal SpO2 measurements showed a mean level of 91% (95% CI 69% –98%). Generally, compensation strategies to cope with both StO2 and SpO2 desaturations were observed in the studied patients. SpO2 had a significant effect on cerebral oxygenation ( p < 0.001), but HR did not, which led to inconclusive results considering different time intervals.

CONCLUSION:

In infants with intermittent hypoxemia and bradycardia, we found a mean StO2 level of 72% and a strong correlation with SpO2. We observed large differences between individuals in the ability to maintain StO2 at a stable level.
The effect of meconium exposure on the expression and differentiation of amniotic fluid mesenchymal stem cells
Authors: Jensen T.J. , Shui J.E. , Finck C.M.
Published: August 2017

BACKGROUND:

The goal of this study was to determine if exposure to meconium would alter the phenotype of amniotic fluid mesenchymal stem cells (AF-MSCs) and the ability of these cells to be differentiated into distal airway type cells.

METHODS:

Meconium was collected, lyophilized and resuspended in PBS at 3 different concentrations (high, medium, and low). AF-MSCs were cultured in the presence of this meconium suspension for 8 hours and then analyzed for changes in gene expression. Additionally, AF-MSCs exposed to meconium were differentiated for 14 days using modified small airway growth medium (mSAGM) and gene expression was determined. As a spontaneous differentiation control, meconium exposed AF-MSCs were cultured in amniotic fluid stem cell medium (AF medium).

RESULTS:

After 8 hours of exposure in culture, AF-MSCs had increased expression of distal airway genes aquaporin 5 (AQP5) and surfactant protein c (SPC) when cultured in AF medium containing meconium. These gene expression levels were similar to that of AF-MSCs that were differentiated in mSAGM for 14 days. Furthermore, there was an up regulation of pluripotency genes NANOG and OCT4 in response to low meconium concentration for 8 hours. Following 14 days of culture in mSAGM, there was an upregulation of TTF1, SPC and AQP5 expression in the control, as well as in the low and medium meconium exposed groups indicating that these cells were still able to be differentiated. High meconium concentration did, however, appear to influence the level of distal airway gene expression after 14 days in mSAGM. After 14 days in AF medium, there was significant downregulation in pluripotency and mesenchymal markers as well as distal airway gene expression in all groups.

CONCLUSION:

The phenotype of AF-MSCs is modulated by meconium exposure; however, the cells were still able to differentiate into distal airway gene and protein expression. This result supports the hypothesis that progenitor cells exist in the amniotic fluid and the presence of meconium may affect their initial phenotype. However, these cells were still able to be differentiated to a distal lung phenotype.
Intestinal perforation in the premature infant
Authors: Vongbhavit K. , Underwood M.A.
Published: August 2017

OBJECTIVE:

To compare demographic data, prenatal and postnatal characteristics, laboratory data, and outcomes in a cohort of premature infants with spontaneous ileal perforation (SIP), surgical necrotizing enterocolitis (sNEC) and matched controls.

METHODS:

A retrospective case–control study of infants with intestinal perforation with a birth weight (BW) less than 2,000 grams and gestational age (GA) less than 34 weeks and infants without perforation matched for BW (±150 grams) and GA (±1week).

RESULTS:

130 premature infants were included, 30 infants with SIP, 35 infants with sNEC and 65 control infants. The median age of onset was 5 days postnatal age in SIP versus 25 days in sNEC ( p < 0.001) and the peak onset was at 26 weeks corrected GA for SIP and 30 weeks corrected GA for sNEC. Infants with perforation had significantly higher rates of mortality ( p < 0.001) and common morbidities associated with prematurity. Administration of corticosteroids and indomethacin did not differ among groups. SIP was more common among infants born to young mothers ( p = 0.04) and less common in infants receiving caffeine ( p = 0.02). sNEC was less common among infants receiving early red cell transfusion ( p = 0.01). Perforation and sNEC trended towards less common in infants receiving inhaled nitric oxide.

CONCLUSION:

SIP and sNEC are distinct clinical entities. Potential protective effects of caffeine, inhaled nitric oxide, and early transfusion should be further studied.
Neuroplasticity and behavioral effects of fluoxetine after experimental stroke
Authors: Sun Yefei , Sun Xiaoyu , Qu Huiling , Zhao Shanshan , Xiao Ting , Zhao Chuansheng
Published: August 2017

The brain can undergo self-repair and has the ability to compensate for functions lost after a stroke. The plasticity of the ischemic brain is influenced by several factors including aging and pharmacotherapy. Fluoxetine is an antidepressant which enhances serotonergic neurotransmission through selective inhibition of neuronal reuptake of serotonin. In clinical practice, fluoxetine alleviates the symptoms of post-stroke depression (PSD), helps motor recovery in stroke patients. In animal experiments, chronic administration of fluoxetine induces increased excitability of mature granule cells (GCs), enhancing axonal and dendritic reorganization, as well as promoting neurogenesis or angiogenesis in the dentate gurus (DG), but the effect of fluoxetine in the subventricular zone (SVZ) remains controversial. Meanwhile, chronic treatment with fluoxetine did not reverse age-dependent suppression of proliferation cells in the DG. Interestingly, although fluoxetine has been found to enhance neurogenesis in the DG in stroke rats, this property is not consistent with the behavioral recovery. More studies into this issue will be required to reveal how to translate enhanced neuronal plasticity into behavioral benefits. This review provides an update of the current knowledge about the neurogenesis and the fate of the newly generated cells after the use of fluoxetine, as well as its ability to promote a behavioral recovery after stroke in clinical and experimental results and attempts to define the therapeutic properties of fluoxetine in regenerative neuroscience.
RETRACTED: A mathematical model to characterize the degree of coalification based on the low-angle region of the X-ray diffractogram
Authors: Yu Jiadong , He Yaqun , Hao Juan , Liu Fengyongzheng , Li Hong , Wang Chao , Wang Haifeng
Published: August 2017

This article has been retracted, and the online PDF has been watermarked “RETRACTION”. The retraction notice is available at http://doi.org/10.3233/XST-221307.
Hybrid Learning for General Type-2 TSK Fuzzy Logic Systems
Authors: Sanchez Mauricio , Castro Juan , Ocegueda-Miramontes Violeta , Cervantes Leticia
Published: August 2017

This work is focused on creating fuzzy granular classification models based on general type-2 fuzzy logic systems when consequents are represented by interval type-2 TSK linear functions. Due to the complexity of general type-2 TSK fuzzy logic systems, a hybrid learning approach is proposed, where the principle of justifiable granularity is heuristically used to define an amount of uncertainty in the system, which in turn is used to define the parameters in the interval type-2 TSK linear functions via a dual LSE algorithm. Multiple classification benchmark datasets were tested in order to assess the quality of the formed granular models; its performance is also compared against other common classification algorithms. Shown results conclude that classification performance in general is better than results obtained by other techniques, and in general, all achieved results, when averaged, have a better performance rate than compared techniques, demonstrating the stability of the proposed hybrid learning technique.
Understanding Starch Structure: Recent Progress
Authors: Bertoft Eric
Published: August 2017

Starch is a major food supply for humanity. It is produced in seeds, rhizomes, roots and tubers in the form of semi-crystalline granules with unique properties for each plant. Though the size and morphology of the granules is specific for each plant species, their internal structures have remarkably similar architecture, consisting of growth rings, blocklets, and crystalline and amorphous lamellae. The basic components of starch granules are two polyglucans, namely amylose and amylopectin. The molecular structure of amylose is comparatively simple as it consists of glucose residues connected through α-(1,4)-linkages to long chains with a few α-(1,6)-branches. Amylopectin, which is the major component, has the same basic structure, but it has considerably shorter chains and a lot of α-(1,6)-branches. This results in a very complex, three-dimensional structure, the nature of which remains uncertain. Several models of the amylopectin structure have been suggested through the years, and in this review two models are described, namely the “cluster model” and the “building block backbone model”. The structure of the starch granules is discussed in light of both models.
Observer-Based Rejection of Cogging Torque Disturbance for Permanent Magnet Motors
Authors: Nam Kyung-Tae , Kim Hyungjong , Lee Seung-Joon , Kuc Tae-Yong
Published: August 2017

In order to prove the speed regulation for permanent magnet motor systems, a control method is proposed to reject the disturbance by cogging torque, which arises from the interaction of the rotor magnets with the steel teeth on the stator. Toward this aim, we present a state observer with an internal model which has harmonics of sinusoids, and it follows from the proposed observer that the information of the cogging disturbance is obtained. It shows that the disturbance is efficiently compensated by a suitable additional control input based on the estimated information. Simulation and experimental results with an actual permanent magnet motor verify the effectiveness of the proposed observer-based algorithm.
Effects of Curing Temperature on Electro-Optical Characteristics of a Polymer-Stabilized In-Plane-Switching Liquid Crystal Cell
Authors: Woo Jae-Hyeon , Choi Tae-Hoon , Jeon Byoung-Gyu , Yoon Tae-Hoon
Published: August 2017

We investigated the electro-optic characteristics of a polymer-stabilized, in-plane-switching (IPS) liquid crystal cell as the UV curing temperature was varied. We found that the response time of an IPS cell could be reduced through low-temperature UV curing of a low concentration of polymer material. We also found that fast switching could be achieved at a low operating voltage and with little light leakage in the dark state. Low-temperature curing of the polymer structure could greatly reduce the average distance between polymer bundles even at low polymer concentrations. Therefore, the decrease in transmittance of a polymer-stabilized IPS cell could be minimized by increasing the cell gap without sacrificing the response time because of the sufficiently high anchoring strength of the polymer structure obtained through low-temperature UV curing.
Particulate Matter and Gaseous Emission of Hydrous Ethanol Gasoline Blends Fuel in a Port Injection Gasoline Engine
Authors: Luo Maji , O. El-Faroug Musaab , Yan Fuwu , Wang Yinan
Published: August 2017

The industrialization that increases day by day needs more and more power/fuel sources that are commonly available, abundant, renewable, and environmentally friendly. Recently, nearly all of the cities in China (PRC) have been influenced by haze. However, the pollutants from automobiles have always been seriously considered to be the main contamination causes of the haze and that influence human health. This study concerns the impact of hydrous ethanol on in-cylinder pressure, particulate matter (PM), and gaseous emissions (oxides of nitrogen (NOx) and unburned hydrocarbon (HC)) from a port fuel injection (PFI) gasoline engine. Tests were conducted on a four-cylinder port injection gasoline engine at different engine loads at an engine speed of 2000 rev/min for commercial gasoline, hydrous ethanol-gasoline blends (10% and 20% hydrous ethanol by volume), and an anhydrous ethanol-gasoline blend (20% anhydrous ethanol by volume). The results show that the peak in-cylinder pressure with the use of gasoline was the highest compared with the hydrous ethanol and anhydrous ethanol blends. Compared with the anhydrous ethanol blend, the hydrous ethanol blends performed well at a high load condition, equivalent to a low load. In addition, the total particulate number (PN) declines with an increase in engine operating loads for all of the fuels tested. The outcome of this study is an important reduction in PM number, mass emissions, and mean diameters of particles as the use of hydrous ethanol blends increases, while the form of the particulate size distribution remains the same. Furthermore, the NOx emission is raised with a rise in engine load, and NOx and HC emissions are reduced with the use of hydrous ethanol and anhydrous ethanol blend as equated with pure gasoline. Moreover, the correlation between the total particle number against NOx and HC has been found; the number of particles increases when the NOx emission decreases, and the opposite trend is exhibited for the HC emission. Therefore, it can be concluded that hydrous ethanol blends look to be a good selection for PM, NOx, and HC reduction for gasoline engines.
Modelling and Simulation of Electric Vehicle Fast Charging Stations Driven by High Speed Railway Systems
Authors: Brenna Morris , Longo Michela , Yaïci Wahiba
Published: August 2017

The aim of this investigation is the analysis of the opportunity introduced by the use of railway infrastructures for the power supply of fast charging stations located in highways. Actually, long highways are often located far from urban areas and electrical infrastructure, therefore the installations of high power charging areas can be difficult. Specifically, the aim of this investigation is the analysis of the opportunity introduced by the use of railway infrastructures for the power supply of fast charging stations located in highways. Specifically, this work concentrates on fast-charging electric cars in motorway service areas by using high-speed lines for supplying the required power. Economic, security, safety and environmental pressures are motivating and pushing countries around the globe to electrify transportation, which currently accounts for a significant amount, above 70 percent of total oil demand. Electric cars require fast-charging station networks to allowing owners to rapidly charge their batteries when they drive relatively long routes. In other words, this means about the infrastructure towards building charging stations in motorway service areas and addressing the problem of finding solutions for suitable electric power sources. A possible and promising solution is proposed in the study that involves using the high-speed railway line, because it allows not only powering a high load but also it can be located relatively near the motorway itself. This paper presents a detailed investigation on the modelling and simulation of a 2 × 25 kV system to feed the railway. A model has been developed and implemented using the SimPower systems tool in MATLAB/Simulink to simulate the railway itself. Then, the model has been applied to simulate the battery charger and the system as a whole in two successive steps. The results showed that the concept could work in a real situation. Nonetheless if more than twenty 100 kW charging bays are required in each direction or if the line topology is changed for whatever reason, it cannot be guaranteed that the railway system will be able to deliver the additional power that is necessary.
Social Environment of Older People during the First Year in Senior Housing and Its Association with Physical Performance
Authors: Lotvonen Sinikka , Kyngäs Helvi , Koistinen Pentti , Bloigu Risto , Elo Satu
Published: August 2017

Increasing numbers of older people relocate into senior housing when their physical performance declines. The change in social environment is known to affect their wellbeing, providing both challenges and opportunities, but more information on the relations between social and physical parameters is required. Thus, we elicited perceptions of the social environment of 81 older people (aged 59–93 years, living in northern Finland) and changes in it 3 and 12 months after relocation to senior housing. We also measured their physical performance, then analysed associations between the social and physical variables. Participants reported that they had freedom to do whatever they liked and generally had enough contact with close people (which have recognized importance for older people’s wellbeing), but changes in their physical condition limited their social activity. Moreover, their usual walking speed, dominant hand’s grip strength and instrumental activities of daily living (IADL) significantly decreased. The pleasantness of the residential community, peer support, constraints on social activity imposed by changes in physical condition, meaningful activity at home and meeting close people all affected these physical performance parameters. Clearly, in addition to assessing physical performance and encouraging regular exercise, the complex interactions among social factors, physical performance and wellbeing should be considered when addressing individuals’ needs.
Contaminations, Sources, and Health Risks of Trace Metal(loid)s in Street Dust of a Small City Impacted by Artisanal Zn Smelting Activities
Authors: Wu Tingting , Bi Xiangyang , Li Zhonggen , Sun Guangyi , Feng Xinbin , Shang Lihai , Zhang Hua , He Tianrong , Chen Ji
Published: August 2017

To investigate the impact of artisanal zinc smelting activities (AZSA) on the distribution and enrichment of trace metal(loid)s in street dust of a small city in Guizhou province, SW China, street dust samples were collected and analyzed for 10 trace metal(loid)s (Cr, Co, Ni, Cu, Zn, As, Cd, Sb, Pb, and Hg). Meanwhile, the health risks of local resident exposed to street dust were assessed. The result showed that the average concentrations of 10 elements were Zn (1039 mg kg−1), Pb (423 mg kg−1), Cr (119 mg kg−1), Cu (99 mg kg−1), As (55 mg kg−1), Ni (39 mg kg−1), Co (18 mg kg−1), Sb (7.6 mg kg−1), Cd (2.6 mg kg−1), and Hg (0.22 mg kg−1). Except Ni, Co, and Cr, other elements in street dust were obviously elevated compared to the provincial soil background. Pb, Zn, Cd, Sb, and Cu were at heavy to moderate contamination status, especially Pb and Zn, with maximums of 1723 and 708 mg kg−1, respectively; As and Hg were slightly contaminated; while Cr, Ni, and Co were at un-contaminated levels. Multivariate statistical analysis revealed AZSA contributed to the increase of Pb, Zn, Cd, Sb, As, and Hg, while, natural sources introduced Ni, Co, Cr, and Cu. The health risk assessment disclosed that children had higher non-carcinogenic risk than those found in adults, and As has hazardous index (HI) higher than 1 both for children and adults, while Pb and Cr only had HIs higher than 1 for children, other elements were relatively safe. For carcinogenic risks, the major concern was As, then a lesser concern for Cr. The study showed that although the scale of AZSA was small, the contamination of heavy metal(loid)s in street dust and associated health risks were severe.
Relationships between Safe Pesticide Practice and Perceived Benefits and Subjective Norm, and the Moderation Role of Information Acquisition: Evidence from 971 Farmers in China
Authors: Wang Jianhua , Deng Yuanyuan , Ma Yuting
Published: August 2017

Improper use of pesticides among farmers has caused food safety issues which are serious threats to public health in China. A central question concerns how to motivate farmers to self-regulate their pesticide usage. The paper aims to identify the influence of an internal driving factor, i.e., perceived benefits, and an external driving factor, i.e., subjective norm, on farmers’ safe pesticide behaviors, and whether the two factors are moderated by the exposure to information on government policies and the market, based on a sample of 971 farmers selected from 5 Chinese provinces. The results revealed that farmers’ safe pesticide usage was predominately driven by perceived benefits whereas external pressure or subjective norm did not play much of a role. Interaction effects were found between the exposure to market information and perceived benefits, and also between subjective norm and exposure to government policy. Extensions agencies are recommended to effectively convey to farmers the benefits to follow safe pesticide practices. Meanwhile, surveillance and monitoring systems should be established so that the prices of their agricultural products are reflected by the quality of the products.
Potential Diagnostic and Therapeutic Applications of Oligonucleotide Aptamers in Breast Cancer
Authors: Wu Xiaoqiu , Shaikh Atik , Yu Yuanyuan , Li Yongshu , Ni Shuaijian , Lu Aiping , Zhang Ge
Published: August 2017

Breast cancer is one of the most common causes of cancer related deaths in women. Currently, with the development of early detection, increased social awareness and kinds of treatment options, survival rate has improved in nearly every type of breast cancer patients. However, about one third patients still have increased chances of recurrence within five years and the five-year relative survival rate in patients with metastasis is less than 30%. Breast cancer contains multiple subtypes. Each subtype could cause distinct clinical outcomes and systemic interventions. Thereby, new targeted therapies are of particular importance to solve this major clinical problem. Aptamers, often termed “chemical antibodies”, are functionally similar to antibodies and have demonstrated their superiority of recognizing target with high selectivity, affinity and stability. With these intrinsic properties, aptamers have been widely studied in cancer biology and some are in clinical trials. In this review, we will firstly discuss about the global impacts and mechanisms of breast cancer, then briefly highlight applications of aptamers that have been developed for breast cancer and finally summarize various challenges in clinical translation of aptamers.
Self-Nanoemulsifying Drug Delivery System for Resveratrol: Enhanced Oral Bioavailability and Reduced Physical Fatigue in Rats
Authors: Yen Ching-Chi , Chang Chih-Wei , Hsu Mei-Chich , Wu Yu-Tse
Published: August 2017

Resveratrol (RES), a natural polyphenolic compound, exerts anti-fatigue activity, but its administration is complicated by its low water solubility. To improve RES bioavailability, this study developed a self-nanoemulsifying drug delivery system (SNEDDS) for RES and evaluated its anti-fatigue activity and rat exercise performance by measuring fatigue-related parameters, namely lactate, ammonia, plasma creatinine phosphokinase, and glucose levels and the swimming time to exhaustion. Through solubility and emulsification testing, the optimized SNEDDS composed of Capryol 90, Cremophor EL, and Tween 20 was developed; the average particle size in this formulation, which had favorable self-emulsification ability, was approximately 41.3 ± 4.1 nm. Pharmacokinetic studies revealed that the oral bioavailability of the optimized RES-SNEDDS increased by 3.2-fold compared with that of the unformulated RES-solution. Pretreatment using the RES-SNEDDS before exercise accelerated the recovery of lactate after exercise; compared with the vehicle group, the plasma ammonia level in the RES-SNEDDS group significantly decreased by 65.4%, whereas the glucose level significantly increased by approximately 1.8-fold. Moreover, the swimming time to exhaustion increased by 2.1- and 1.8-fold, respectively, compared with the vehicle and RES-solution pretreatment groups. Therefore, the developed RES-SNEDDS not only enhances the oral bioavailability of RES but may also exert anti-fatigue pharmacological effect.
PSFM-DBT: Identifying DNA-Binding Proteins by Combing Position Specific Frequency Matrix and Distance-Bigram Transformation
Authors: Zhang Jun , Liu Bin
Published: August 2017

DNA-binding proteins play crucial roles in various biological processes, such as DNA replication and repair, transcriptional regulation and many other biological activities associated with DNA. Experimental recognition techniques for DNA-binding proteins identification are both time consuming and expensive. Effective methods for identifying these proteins only based on protein sequences are highly required. The key for sequence-based methods is to effectively represent protein sequences. It has been reported by various previous studies that evolutionary information is crucial for DNA-binding protein identification. In this study, we employed four methods to extract the evolutionary information from Position Specific Frequency Matrix (PSFM), including Residue Probing Transformation (RPT), Evolutionary Difference Transformation (EDT), Distance-Bigram Transformation (DBT), and Trigram Transformation (TT). The PSFMs were converted into fixed length feature vectors by these four methods, and then respectively combined with Support Vector Machines (SVMs); four predictors for identifying these proteins were constructed, including PSFM-RPT, PSFM-EDT, PSFM-DBT, and PSFM-TT. Experimental results on a widely used benchmark dataset PDB1075 and an independent dataset PDB186 showed that these four methods achieved state-of-the-art-performance, and PSFM-DBT outperformed other existing methods in this field. For practical applications, a user-friendly webserver of PSFM-DBT was established, which is available at http://bioinformatics.hitsz.edu.cn/PSFM-DBT/.
CCR7 Sulfotyrosine Enhances CCL21 Binding
Authors: Phillips Andrew , Taleski Deni , Koplinski Chad , Getschman Anthony , Moussouras Natasha , Richard Amanda , Peterson Francis , Dwinell Michael , Volkman Brian , Payne Richard , Veldkamp Christopher
Published: August 2017

Chemokines are secreted proteins that direct the migration of immune cells and are involved in numerous disease states. For example, CCL21 (CC chemokine ligand 21) and CCL19 (CC chemokine ligand 19) recruit antigen-presenting dendritic cells and naïve T-cells to the lymph nodes and are thought to play a role in lymph node metastasis of CCR7 (CC chemokine receptor 7)-expressing cancer cells. For many chemokine receptors, N-terminal posttranslational modifications, particularly the sulfation of tyrosine residues, increases the affinity for chemokine ligands and may contribute to receptor ligand bias. Chemokine sulfotyrosine (sY) binding sites are also potential targets for drug development. In light of the structural similarity between sulfotyrosine and phosphotyrosine (pY), the interactions of CCL21 with peptide fragments of CCR7 containing tyrosine, pY, or sY were compared using protein NMR (nuclear magnetic resonance) spectroscopy in this study. Various N-terminal CCR7 peptides maintain binding site specificity with Y8-, pY8-, or sY8-containing peptides binding near the α-helix, while Y17-, pY17-, and sY17-containing peptides bind near the N-loop and β3-stand of CCL21. All modified CCR7 peptides showed enhanced binding affinity to CCL21, with sY having the largest effect.
Development and Properties of Valine-Alanine based Antibody-Drug Conjugates with Monomethyl Auristatin E as the Potent Payload
Authors: Wang Yanming , Fan Shiyong , Zhong Wu , Zhou Xinbo , Li Song
Published: August 2017

Antibody-drug conjugates (ADCs), designed to selectively deliver cytotoxic agents to antigen-bearing cells, are poised to become an important class of cancer therapeutics. Human epithelial growth factor receptor (HER2) is considered an effective target for cancer treatment, and a HER2-targeting ADC has shown promising results. Most ADCs undergoing clinical evaluation contain linkers that have a lysosomal protease-cleavable dipeptide, of which the most common is valine-citrulline (VC). However, valine-alanine (VA), another dipeptide comprising two human essential amino acids, has been used in next generation ADCs loading new toxins, but the druggable properties of ADCs loaded the most popular monomethyl auristatin E (MMAE) remain to be further explored. In this study, we generated VA-based ADCs that connected MMAE to an anti-HER2 antibody. We studied the differences in the preparation process, in vitro stability, cathepsin B activity and in vitro cytotoxicity of VA-based ADC compared to the ADC of VC. VA had comparable performance to VC, which preliminarily displays its practicability. Additional efficacy and safety studies in a xenograft model indicate this novel ADC exerted potent anti-tumor activity and negligible toxicity. The results of this study show the application potential of VA-based ADC with MMAE as the payload.
Experimental Investigation on Surface Quality Processed by Self-Excited Oscillation Pulsed Waterjet Peening
Authors: Ding Xiaolong , Kang Yong , Li Deng , Wang Xiaochuan , Zeng Dongping
Published: August 2017

High-speed waterjet peening technology has attracted a lot of interest and is now being widely studied due to its great ability to strengthen metal surfaces. In order to further improve the mechanical properties of metals, self-excited oscillation pulsed waterjets (SOPWs) were used for surface peening with an experimental investigation focused on the surface topography and properties. By impinging the aluminum alloy (5052) specimens with SOPWs issuing from an organ-pipe oscillation nozzle, the hardness and roughness at various inlet pressures and stand-off distances were measured and analyzed, as well as the residual stress. Under the condition of optimum stand-off distances, the microscopic appearances of peened specimens obtained by SEM were displayed and analyzed. Results show that self-excited oscillation pulsed waterjet peening (SOPWP) is capable of improving the surface quality. More specifically, compared with an untreated surface, the hardness and residual stress of the peened surfaces were increased by 61.69% and 148%, respectively. There exists an optimal stand-off distance and operating pressure for creating the highest surface quality. SOPWP can produce almost the same enhancement effect as shot peening and lead to a lower surface roughness. Although such an approach is empirical and qualitative in nature, this procedure also generated information of value in guiding future theoretical and experimental work on the application of SOPWP in the industry practice.
Heat-Assisted Multiferroic Solid-State Memory
Authors: Lepadatu Serban , Vopson Melvin
Published: August 2017

A heat-assisted multiferroic solid-state memory design is proposed and analysed, based on a PbNbZrSnTiO3 antiferroelectric layer and Ni81Fe19 magnetic free layer. Information is stored as magnetisation direction in the free layer of a magnetic tunnel junction element. The bit writing process is contactless and relies on triggering thermally activated magnetisation switching of the free layer towards a strain-induced anisotropy easy axis. A stress is generated using the antiferroelectric layer by voltage-induced antiferroelectric to ferroelectric phase change, and this is transmitted to the magnetic free layer by strain-mediated coupling. The thermally activated strain-induced magnetisation switching is analysed here using a three-dimensional, temperature-dependent magnetisation dynamics model, based on simultaneous evaluation of the stochastic Landau-Lifshitz-Bloch equation and heat flow equation, together with stochastic thermal fields and magnetoelastic contributions. The magnetisation switching probability is calculated as a function of stress magnitude and maximum heat pulse temperature. An operating region is identified, where magnetisation switching always occurs, with stress values ranging from 80 to 180 MPa, and maximum temperatures normalised to the Curie temperature ranging from 0.65 to 0.99.
Preparation of Luminescent Metal-Organic Framework Films by Soft-Imprinting for 2,4-Dinitrotoluene Sensing
Authors: Roales Javier , Moscoso Francisco , Gámez Francisco , Lopes-Costa Tânia , Sousaraei Ahmad , Casado Santiago , Castro-Smirnov Jose , Cabanillas-Gonzalez Juan , Almeida José , Queirós Carla , Cunha-Silva Luís , Silva Ana , Pedrosa José
Published: August 2017

A novel technique for the creation of metal-organic framework (MOF) films based on soft-imprinting and their use as gas sensors was developed. The microporous MOF material [Zn2(bpdc)2(bpee)] (bpdc = 4,4′-biphenyldicarboxylate; bpee = 1,2-bipyridylethene) was synthesized solvothermally and activated by removing the occluded solvent molecules from its inner channels. MOF particles were characterized by powder X-ray diffraction and fluorescence spectroscopy, showing high crystallinity and intense photoluminescence. Scanning electron microscope images revealed that MOF crystals were mainly in the form of microneedles with a high surface-to-volume ratio, which together with the high porosity of the material enhances its interaction with gas molecules. MOF crystals were soft-imprinted into cellulose acetate (CA) films on quartz at different pressures. Atomic force microscope images of soft-imprinted films showed that MOF crystals were partially embedded into the CA. With this procedure, mechanically stable films were created, with crystals protruding from the CA surface and therefore available for incoming gas molecules. The sensing properties of the films were assessed by exposing them to saturated atmospheres of 2,4-dinitrotoluene, which resulted in a substantial quenching of the fluorescence after few seconds. The soft-imprinted MOF films on CA/quartz exhibit good sensing capabilities for the detection of nitroaromatics, which was attributed to the MOF sensitivity and to the novel and more efficient film processing method based on soft-imprinting.
Recommendations for Improving Identification and Quantification in Non-Targeted, GC-MS-Based Metabolomic Profiling of Human Plasma
Authors: Wang Hanghang , Muehlbauer Michael , O’Neal Sara , Newgard Christopher , Hauser Elizabeth , Bain James , Shah Svati
Published: August 2017

The field of metabolomics as applied to human disease and health is rapidly expanding. In recent efforts of metabolomics research, greater emphasis has been placed on quality control and method validation. In this study, we report an experience with quality control and a practical application of method validation. Specifically, we sought to identify and modify steps in gas chromatography-mass spectrometry (GC-MS)-based, non-targeted metabolomic profiling of human plasma that could influence metabolite identification and quantification. Our experimental design included two studies: (1) a limiting-dilution study, which investigated the effects of dilution on analyte identification and quantification; and (2) a concentration-specific study, which compared the optimal plasma extract volume established in the first study with the volume used in the current institutional protocol. We confirmed that contaminants, concentration, repeatability and intermediate precision are major factors influencing metabolite identification and quantification. In addition, we established methods for improved metabolite identification and quantification, which were summarized to provide recommendations for experimental design of GC-MS-based non-targeted profiling of human plasma.
Radiolabeled Dendrimers for Nuclear Medicine Applications
Authors: Zhao Lingzhou , Zhu Meilin , Li Yujie , Xing Yan , Zhao Jinhua
Published: August 2017

Recent advances in nuclear medicine have explored nanoscale carriers for targeted delivery of various radionuclides in specific manners to improve the effect of diagnosis and therapy of diseases. Due to the unique molecular architecture allowing facile attachment of targeting ligands and radionuclides, dendrimers provide versatile platforms in this filed to build abundant multifunctional radiolabeled nanoparticles for nuclear medicine applications. This review gives special focus to recent advances in dendrimer-based nuclear medicine agents for the imaging and treatment of cancer, cardiovascular and other diseases. Radiolabeling strategies for different radionuclides and several challenges involved in clinical translation of radiolabeled dendrimers are extensively discussed.
The Role of Kinase Modulators in Cellular Senescence for Use in Cancer Treatment
Authors: Lee Chang , Baek Juhwa , Han Sun-Young
Published: August 2017

Recently, more than 30 small molecules and eight monoclonal antibodies that modulate kinase signaling have been approved for the treatment of several pathological conditions, including cancer, idiopathic pulmonary fibrosis, and rheumatoid arthritis. Among them, kinase modulators have been a primary focus for use in cancer treatment. Cellular senescence is believed to protect cells from tumorigenesis by irreversibly halting cell cycle progression and avoiding the growth of damaged cells and tissues. Senescence can also contribute to tumor suppression and be utilized as a mechanism by anti-cancer agents. Although the role of kinase modulators in cancer treatment and their effects on senescence in tumor development have been extensively studied, the relationship between kinase modulators for cancer treatment and senescence has not been fully discussed. In this review, we discuss the pro- and anti-tumorigenesis functions of senescence and summarize the key roles of kinase modulators in the regulation of senescence against tumors.