bluuebunny/crossref_metadata_2025 huggingface dataset:
Adaptive Differential Evolution Algorithm Based on Fitness Landscape Characteristic
Authors: Zheng Liming , Luo Shiqi
Published: May 2022

Differential evolution (DE) is a simple, effective, and robust algorithm, which has demonstrated excellent performance in dealing with global optimization problems. However, different search strategies are designed for different fitness landscape conditions to find the optimal solution, and there is not a single strategy that can be suitable for all fitness landscapes. As a result, developing a strategy to adaptively steer population evolution based on fitness landscape is critical. Motivated by this fact, in this paper, a novel adaptive DE based on fitness landscape (FL-ADE) is proposed, which utilizes the local fitness landscape characteristics in each generation population to (1) adjust the population size adaptively; (2) generate DE/current-to-pcbest mutation strategy. The adaptive mechanism is based on local fitness landscape characteristics of the population and enables to decrease or increase the population size during the search. Due to the adaptive adjustment of population size for different fitness landscapes and evolutionary processes, computational resources can be rationally assigned at different evolutionary stages to satisfy diverse requirements of different fitness landscapes. Besides, the DE/current-to-pcbest mutation strategy, which randomly chooses one of the top p% individuals from the archive cbest of local optimal individuals to be the pcbest, is also an adaptive strategy based on fitness landscape characteristic. Using the individuals that are approximated as local optimums increases the algorithm’s ability to explore complex multimodal functions and avoids stagnation due to the use of individuals with good fitness values. Experiments are conducted on CEC2014 benchmark test suit to demonstrate the performance of the proposed FL-ADE algorithm, and the results show that the proposed FL-ADE algorithm performs better than the other seven highly performing state-of-art DE variants, even the winner of the CEC2014 and CEC2017. In addition, the effectiveness of the adaptive population mechanism and DE/current-to-pcbest mutation strategy based on landscape fitness proposed in this paper are respectively verified.
The Trefoil Soliton
Authors: Singer David A.
Published: May 2022

The Kiepert trefoil is an algebraic curve with remarkable geometric and number theoretic properties. Ludwig Kiepert, generalizing ideas due to Serret and Liouville, determined that it could be parametrized by arc length in terms of elliptic functions. In this note, we observe some other properties of the curve. In particular, the curve is a special example of a buckled ring, and thus a solitary wave solution to the planar filament equation, evolving by rotation. It is also a solitary wave solution to a flow in the (three-dimensional) filament hierarchy, evolving by translation.
The Effects of 32 Weeks of Multicomponent Training with Different Exercises Order in Elderly Women’s Functional Fitness and Body Composition
Authors: Monteiro António M. , Rodrigues Sandra , Matos Sérgio , Teixeira José E. , Barbosa Tiago M. , Forte Pedro
Published: May 2022

Background and objectives: Starting the multicomponent training sessions with aerobic-based exercises or resistance-based exercises may have different effects on functional fitness and body composition. Thus, the aim of this study was to assess the effects of the order of exercises in elderly women’s physical fitness and body composition by multicomponent training. Materials and Methods: A sample of 91 elderly females, aged between 60 and 81, were randomly divided into three groups (A, B, C). Each group performed the following order of exercises: Group A consisted of warm-up followed by aerobic training, strength training, stretching and cool down; Group B consisted of warm-up followed by strength training, aerobic training, stretching and cool down; while the control group (C) did not perform any exercise. Functional fitness and body composition were assessed at 3 moments of the 32 weeks (baseline and after each 16-week) intervention. One-way ANOVA for comparison between groups, ANOVA for repeated measures and multiple linear regression were used for statistical analysis. Results: The results showed that the functional fitness and body composition varied over the 32 weeks of multicomponent training. However, group A seems to show higher improvements in more variables. Conclusion: In the current study, group A obtained better results in most of the evaluated parameters. Thus, to improve functional fitness, warm-up, followed by aerobic training, strength training and relaxation may be the most suitable training for elderly women.
Treatment of Early-Stage Gynecological Cancer-Related Lower Limb Lymphedema by Lymphaticovenular Anastomosis—The Triple Incision Approach
Authors: Caretto Anna Amelia , Stefanizzi Gianluigi , Garganese Giorgia , Fragomeni Simona Maria , Federico Alex , Tagliaferri Luca , Fionda Bruno , Cina Alessandro , Scambia Giovanni , Gentileschi Stefano
Published: May 2022

Background and Objectives: Lower extremity lymphedema (LEL) is one of the most relevant chronic and disabling sequelae after gynecological cancer therapy involving pelvic lymphadenectomy (PL). Supermicrosurgical lymphaticovenular anastomosis (LVA) is a safe and effective procedure to treat LEL, particularly indicated in early-stage cases when conservative therapies are insufficient to control the swelling. Usually, preoperative assessment of these patients shows patent and peristaltic lymphatic vessels that can be mapped throughout the limb to plan the sites of skin incision to perform LVA. The aim of this study is to report the efficacy of our approach based on planning LVA in three areas of the lower limb in improving early-stage gynecological cancer-related lymphedema (GCRL) secondary to PL. Materials and Methods: We retrospectively reviewed the data of patients who underwent LVA for the treatment of early-stage GCRL following PL. Patients who had undergone groin dissection were excluded. Our preoperative study based on indocyanine green lymphography (ICG-L) and color doppler ultrasound (CDU) planned three incision sites located in the groin, in the medial surface of the distal third of the thigh, and in the upper half of the leg, to perform LVA. The primary outcome measure was the variation of the mean circumference of the limb after surgery. The changes between preoperative and postoperative limbs’ measures were analyzed by Student’s t-test. p values < 0.05 were considered significant. Results: Thirty-three patients were included. In every patient, three incision sites were employed to perform LVA. A total of 119 LVA were established, with an average of 3.6 for each patient. The mean circumference of the operated limb showed a significant reduction after surgery, decreasing from 37 cm ± 4.1 cm to 36.1 cm ± 4.4 (p < 0.01). Conclusions: Our results suggest that in patients affected by early-stage GCRL secondary to PL, the placement of incision sites in all the anatomical subunits of the lower limb is one of the key factors in achieving good results after LVA.
Comparative Evaluation of Influence of Nd:YAG Laser (1064 nm) and 980 nm Diode Laser on Enamel around Orthodontic Brackets: An In Vitro Study
Authors: Mocuta(Bojoga) Daliana-Emanuela , Grad(Buriac) Oana , Mateas Marius , Luca Ruxandra , Carmen Todea Darinca
Published: May 2022

(1) Background: The prevention of demineralizing lesions at the enamel structure level continues to represent a challenge in daily dental practice. When bacteria influence the pH level, this will decrease below the threshold for remineralization and the dissociation of hydroxyapatite will occur with a high percentage of phosphate and calcium loss. These elements continue to be studied by many authors in order to obtain a working protocol that will lead to their stabilization at the level of the enamel structure, thus preventing the demineralization process. The aim of this study is to evaluate and compare the influence of two types of laser wavelengths on the surface morphology and mineral components of the enamel through an examination with scanning electron microscopy (SEM) and energy-dispersive X-ray spectrometry (EDX). (2) Methods: Thirty permanent human incisors extracted for periodontal reasons from patients aged 25–40 years old were selected for this study. Metallic brackets (SS Standard 022 Slot, OC Orthodontics, McMinnville, OR, 97128, USA) were bonded onto each tooth. The buccal surface was randomly assigned three sections: Section A—negative control (no treatment), section B—treated with 980 nm Gallium–Aluminum–Arsenide diode laser (a 300 µm optic fiber was used with 0.8 W output power, energy density of 5.33 J/mm2, in continuous mode, for 30 s, oriented perpendicularly to the enamel surface in contact mode) (KaVo GENTLEray 980 Diode Laser, Kaltenbach & Voigt GmbH, Biberach, Germany), and section C—treated with Nd:YAG laser (a 300 µm fiber was carried out at a 1 mm distance from the enamel surface with 0.75 W power, 75 mJ pulse energy, pulse repetition rate of 10 Hz, 5 J/mm2 fluency, average exposure time of 30 s, and water cooling assisted) (LIGHTWALKER AT S, M021-5AF/1 S, Fotona d.o.o, Ljubljana, Slovenia). The elements evaluated in this study were calcium (Ca), phosphate (P), oxygen (O), and carbon (C). A one-way analysis of variance, paired t-tests, and independent t-tests were carried out to evaluate the results using the SPSS 19 IBM Statistical package software for Microsoft. (3) Results: The evaluation of the data indicated that both wavelengths produced an increase in Ca wt% (for diode laser the mean of Ca wt% before irradiation was 21.06, while that after treatment reached 28.24; and for Nd:YAG laser, the mean of Ca wt% before irradiation was 21.31, while that after treatment reached 33.88); as well, the 980 nm diode laser decreased P wt% (from 17.20 before irradiation to 16.92 after irradiation) and the Nd:YAG laser increased P wt% (from 17.46 before irradiation to 18.28 after irradiation). These results showed a statistically significant difference at the p < 0.05 level. (4) Conclusions: It can be concluded that the best improvement of enamel chemical composition was obtained with Nd:YAG irradiation.
DNA Nanotechnology on Bio-Membranes
Authors: Zhao Bin , You Mingxu
Published: May 2022

In recent years, DNA nanotechnology, including both structural and dynamic DNA nanotechnology, has emerged as a powerful tool for various analytical and biomedical applications in biological membranes [...]
Separation of Mercury(II) from Industrial Wastewater through Polymer Inclusion Membranes with Calix[4]pyrrole Derivative
Authors: Zawierucha Iwona , Nowik-Zajac Anna , Lagiewka Jakub , Malina Grzegorz
Published: May 2022

Polymer membranes with immobilized ligands are encouraging alternatives for the removal of toxic metal ions from aquatic waste streams, including industrial wastewater, in view of their high selectivity, stability, removal efficacy and low energy demands. In this study, polymer inclusion membranes (PIMs) based on cellulose triacetate, with a calix[4]pyrrole derivative as an ion carrier, were tested for their capability to dispose mercury (Hg(II)) ions from industrial wastewater. The impacts were assessed relative to carrier content, the quantity of plasticizer in the membrane, the hydrocholoric acid concentration in the source phase, and the character of the receiving phase on the performance of Hg(II) elimination. Optimally designed PIMs could be an interesting option for the industrial wastewater treatment due to the high removal efficiency of Hg(II) and great repeatability.
Understanding the Dynamics of the Transient and Permanent Opening Events of the Mitochondrial Permeability Transition Pore with a Novel Stochastic Model
Authors: Yalamanchili Keertana , Afzal Nasrin , Boyman Liron , Mannella Carmen A. , Lederer W. Jonathan , Jafri M. Saleet
Published: May 2022

The mitochondrial permeability transition pore (mPTP) is a non-selective pore in the inner mitochondrial membrane (IMM) which causes depolarization when it opens under conditions of oxidative stress and high concentrations of Ca2+. In this study, a stochastic computational model was developed to better understand the dynamics of mPTP opening and closing associated with elevated reactive oxygen species (ROS) in cardiomyocytes. The data modeled are from “photon stress” experiments in which the fluorescent dye TMRM (tetramethylrhodamine methyl ester) is both the source of ROS (induced by laser light) and sensor of the electrical potential difference across the IMM. Monte Carlo methods were applied to describe opening and closing of the pore along with the Hill Equation to account for the effect of ROS levels on the transition probabilities. The amplitude distribution of transient mPTP opening events, the number of transient mPTP opening events per minute in a cell, the time it takes for recovery after transient depolarizations in the mitochondria, and the change in TMRM fluorescence during the transition from transient to permanent mPTP opening events were analyzed. The model suggests that mPTP transient open times have an exponential distribution that are reflected in TMRM fluorescence. A second multiple pore model in which individual channels have no permanent open state suggests that 5–10 mPTP per mitochondria would be needed for sustained mitochondrial depolarization at elevated ROS with at least 1 mPTP in the transient open state.
Performance Comparison of Cross- and Forward-Flow Configurations for Multiple-Effect Vacuum Membrane Distillation
Authors: Najib Abdullah , Al-Ansary Hany , Orfi Jamel , Ali Emad , Almehmadi Fahad Awjah
Published: May 2022

This work addresses retrofitting the infrastructure of multiple-effect vacuum membrane distillation (V-MEMD) units by using cross-flow configuration (CFC). In this configuration, the feed water is evenly divided and distributed over the effects. In this case, the feed water stream for each effect is kept at a high temperature and low flow rate. This will lead to an increase in the vapor pressure gradient across the hydrophobic membrane and can also maintain the thermal energy of the stream inside the individual effect. It is found that CFC improves internal and global performance indicators of productivity, energy, and exergy. A mathematical model was used to investigate the performance of such a modification as compared to the forward-flow configuration (FFC). The cross-flow configuration led to a clear improvement in the internal performance indicators of the V-MEMD unit, where specifically the mass flux, recovery ratio, gain output ratio, and heat recovery factor were increased by 2 to 3 folds. Moreover, all the global performance indicators were also enhanced by almost 2 folds, except for the performance indicators related to the heat pump, which is used to cool the cold water during the operation of the V-MEMD unit. For the heat pump system, the specific electrical energy consumption, SEEC, and the exergy destruction percentage, Ψdes, under the best-operating conditions, were inferior when the feed water flow was less than 159 L/h. This can be attributed to the fact that the heat rejected from the heat pump system is not fully harnessed.
Microstructure, Inclusions, and Elemental Distribution of a Compacted Graphite Iron Alloyed by Ce and La Rare Earth (RE) Elements
Authors: Fan Zengwei , Zhu Jianan , Lian Xintong , Liu Tengshi , Xu Dexiang , Wei Xicheng , Dong Han
Published: May 2022

This work investigates the microstructure and inclusions of a compacted graphite iron (CGI) alloyed by Ce and La rare earth (RE) elements. In our study, alloying elemental distribution and solute segregation were characterized by methods of secondary ion mass spectrometry (SIMS) and a three-dimensional atom probe (3DAP) with high sensitivity and spatial resolution. RE sulfide, MgS, carbide, and composite inclusions formed during solidification and provided heterogeneous nucleation cores for the nucleation of the graphite. Significant solute clustering in the matrix, coupled with the segregation of solute to grain boundaries, was observed. C, Mn, Cr, and V were soluted in cementite and promoted the precipitation of cementite, while Si was found to be soluted in ferrite. Cu is usually distributed uniformly in ferrite, but some Cu-rich atom clusters were observed to segregate towards the interface between the ferrite and cementite, stabilizing the pearlite. In addition, P, as a segregation element, was enriched along the boundaries continuously. The RE elements participated in the formation of inclusions, consuming harmful elements such as As and P, and also promoted the heterogeneous nucleation of the graphite and segregated, in the form of solute atoms, at its interfaces.
A Probabilistic Fatigue Strength Assessment in AlSi-Cast Material by a Layer-Based Approach
Authors: Oberreiter Matthias , Fladischer Stefan , Stoschka Michael , Leitner Martin
Published: May 2022

An advanced lightweight design in cast aluminium alloys features complexly shaped geometries with strongly varying local casting process conditions. This affects the local microstructure in terms of porosity grade and secondary dendrite arm spacing distribution. Moreover, complex service loads imply changing local load stress vectors within these components, evoking a wide range of highly stressed volumes within different microstructural properties per load sequence. To superimpose the effects of bulk and surface fatigue strength in relation to the operating load sequence for the aluminium alloy EN AC 46200, a layer-based fatigue assessment concept is applied in this paper considering a non-homogeneous distribution of defects within the investigated samples. The bulk fatigue property is now obtained by a probabilistic evaluation of computed tomography results per investigated layer. Moreover, the effect of clustering defects of computed tomography is studied according to recommendations from the literature, leading to a significant impact in sponge-like porosity layers. The highly stressed volume fatigue model is applied to computed tomography results. The validation procedure leads to a scattering of mean fatigue life from −2.6% to 12.9% for the investigated layers, inheriting strongly varying local casting process conditions.
Effect of Surface Mechanical Attrition Treatment on Torsional Fatigue Properties of a 7075 Aluminum Alloy
Authors: Li Yizhuo , Retraint Delphine , Gao Pengfei , Xue Hongqian , Gao Tao , Sun Zhidan
Published: May 2022

The effect of Surface Mechanical Attrition Treatment (SMAT) on torsional fatigue properties of a 7075 aluminum alloy was investigated. A number of fatigue samples were heat treated to increase the sensitivity of the material to SMAT. Compared with the as-machined (AM) samples, the fatigue lives of their SMATed counterparts (AM-SMAT) tested under torsional loading increased under high stress amplitudes, but decreased under low amplitudes. However, the fatigue lives of heated and SMATed samples (HT-SMAT) increased under all the investigated stress amplitudes, compared with those that were heat treated (HT). It was also revealed that the cracking mechanisms are different for the samples in different states, and they are dependent on the imposed stress levels. The results show that SMAT could have both beneficial and detrimental effects on the fatigue lives depending on the testing conditions. The roles played by various factors, including residual stresses, grain refinement, and surface roughness, were analyzed and discussed to interpret the results.
3,3′-Diindolylmethane Enhances Fluorouracil Sensitivity via Inhibition of Pyrimidine Metabolism in Colorectal Cancer
Authors: Zhang Jieping , Zou Shaomin , Zhang Yijing , Yang Ziqing , Wang Wencong , Meng Manqi , Feng Junyan , Zhang Peng , Xiao Lishi , Lee Mong-Hong , Fang Lekun
Published: May 2022

Chemoresistance limits treatment outcomes in colorectal cancer (CRC) patients. A dimeric metabolite of indole-3-carbinol, 3,3′-diindolylmethane (DIM) is abundant in cruciferous vegetables and has shown anticancer efficacy. The role of DIM in regulating chemosensitivity in CRC remains unknown. In this study, we demonstrated that DIM treatment inhibits the malignant progression of CRC. RNA sequencing indicated that pyrimidine synthesis genes are attenuated by DIM treatment. Stable 13C-labeled glucose tracing revealed that DIM inhibits de novo pyrimidine biosynthesis in CRC. DIM increases 5-FU cytotoxicity in CRC via regulation of the expression of pyrimidine metabolism-related genes. DIM synergizes with 5-FU to enhance its inhibitory effects on CRC both in vivo and in vitro. Our results suggest that DIM improves the therapeutic outcomes of FU-based chemotherapy in CRCs by inhibiting pyrimidine metabolism, identifying a new strategy for clinical therapy.
Research on the High Sensitivity Detection Method of Carbon Nanotube/Polydimethylsiloxane Composites Structure
Authors: Liu Lishuang , Wang Ruirong , Guo Hao , Liu Jinping , Li Xin , Qin Yue , Tang Jun
Published: May 2022

In this paper, a carbon nanotube (CNT)/polydimethylsiloxane (PDMS) composite force-sensitive structure with good flexibility is proposed and fabricated, and the measurement of scanning electron microscopy (SEM) and Raman are carried out. The equivalent circuit of force-sensitive test of structure is performed and analyzed under direct current (DC) and alternating current (AC) conditions. Under AC conditions, experimental results further show that the sensitivity and sensitivity factors of force-sensitive structures are 0.15 KPa−1 and 2.17 in the pressure range of 600–1000 KPa compressive stress and 20–50% tensile stress, respectively. These results are increased by 36.4% and 38.2% compared to the results of compressive stress (0.11 KPa−1) and tensile stress (1.57) under DC conditions, respectively. It shows that the carbon nanotube/PDMS composite has higher test accuracy under AC conditions.
Effect of Wind-Induced Vibration on Measurement Range of Microcantilever Anemometer
Authors: Ye Yizhou , Wan Shu , He Xuefeng
Published: May 2022

In this paper, the effect of wind-induced vibration on measurement range of microcantilever anemometer is investigated for the first time. The microcantilever anemometer is composed of a flexible substrate and a piezoresistor. The wind speed can be detected through the airflow-induced deformation in the flexible substrate. Previous work indicated that the flexible substrate vibrates violently once the wind speed exceeds a critical value, resulting in severe output jitter. This wind-induced vibration limits the measurement range of the anemometer, and the relationship between the anemometer measurement range and its structural parameters has not been explored systematically. Therefore, this paper aims to reveal this relationship theoretically and experimentally, demonstrating that a shorter and thicker cantilever with larger stiffness can effectively suppress the wind-induced vibration, leading to the critical speed rising. By eliminating the wind-induced vibration, the measurement range of the microcantilever anemometer can be increased by up to 697%. These results presented in this paper can pave the way for the design and fabrication of wide-range mechanical anemometers.
Experimental Study on Texture Coupling Mechanism and Antifriction Performance of Piston Rod Seal Pair
Authors: Tang Jie , Zeng Jie , Lu Xin
Published: May 2022

The effect of the coupling texture on the friction and wear of a piston rod-rubber seal pair under lubricating conditions is studied in this paper. Crescentiform textures with different area densities were fabricated on high carbon chromium bearing steel (GCr15) and ethylene propylene diene monomer (EPDM) materials by using a laser marking machine. We compare and analyze the effects of untextured, single-textured, and coupling-textured surfaces on the friction characteristics of the piston rod-rubber seal pair by conducting tests on the reciprocating module of the UMT-2 friction and wear testing machine. The results showed that the coupling-textured surface had the lowest coefficient of friction and wear compared to the untextured and single-textured surfaces. When the normal load was 10 N under the optimal coupling texture area density (6.4%), the friction and wear of the sealing pair decreased the most. Compared with the untextured surface, the friction coefficient was reduced by 27.9% and the wear amount was reduced by 30.0%; compared with the single-textured surface, the friction coefficient was reduced by 18.9%, and the wear amount was reduced by 23.8%. The coupling effect generated by the coupling texture effectively enhanced the formation and stabilization of the oil lubricant film and effectively captured wear debris, preventing it from continuously scratching the surface and reducing wear and roughness.
A Magnetorheological Duckbill Valve Micropump for Drug Delivery Applications
Authors: Hassan Rubayet , Cesmeci Sevki , Baniasadi Mahmoud , Palacio Anthony , Robbins Austin
Published: May 2022

In this study, we propose a duckbill valve microfluidic pump that relies on an electromagnetic actuation mechanism. An FEA/CFD-based approach was adopted for the design of the device due to the coupled electromagnetic–solid–fluid interactions in the device. The simulation methodology was confirmed with the previously published data in the literature to ensure the accuracy of the simulations. The proposed optimum duckbill valve micropump can pump 2.45 µL of fluid during the first 1 s, including both contraction and expansion phases, almost 16.67% more than the basic model. In addition, the model can pump a maximum volume of 0.26 µL of fluid at the end of the contraction phase (at 0.5 s) when the magnetic flux density is at maximum (0.027 T). The use of a duckbill valve in the model also reduces the backflow by almost 7.5 times more than the model without any valve. The proposed device could potentially be used in a broad range of applications, such as an insulin dosing system for Type 1 diabetic patients, artificial organs to transport blood, organ-on-chip applications, and so on.
Conductive Bridge Random Access Memory (CBRAM): Challenges and Opportunities for Memory and Neuromorphic Computing Applications
Authors: Abbas Haider , Li Jiayi , Ang Diing
Published: May 2022

Due to a rapid increase in the amount of data, there is a huge demand for the development of new memory technologies as well as emerging computing systems for high-density memory storage and efficient computing. As the conventional transistor-based storage devices and computing systems are approaching their scaling and technical limits, extensive research on emerging technologies is becoming more and more important. Among other emerging technologies, CBRAM offers excellent opportunities for future memory and neuromorphic computing applications. The principles of the CBRAM are explored in depth in this review, including the materials and issues associated with various materials, as well as the basic switching mechanisms. Furthermore, the opportunities that CBRAMs provide for memory and brain-inspired neuromorphic computing applications, as well as the challenges that CBRAMs confront in those applications, are thoroughly discussed. The emulation of biological synapses and neurons using CBRAM devices fabricated with various switching materials and device engineering and material innovation approaches are examined in depth.
Asymptotic Synchronization of Memristive Cohen-Grossberg Neural Networks with Time-Varying Delays via Event-Triggered Control Scheme
Authors: Yao Wei , Yu Fei , Zhang Jin , Zhou Ling
Published: May 2022

This paper investigates the asymptotic synchronization of memristive Cohen-Grossberg neural networks (MCGNNs) with time-varying delays under event-triggered control (ETC). First, based on the designed feedback controller, some ETC conditions are provided. It is demonstrated that ETC can significantly reduce the update times of the controller and decrease the computing cost. Next, some sufficient conditions are derived to ensure the asymptotic synchronization of MCGNNs with time-varying delays under the ETC method. Finally, a numerical example is provided to verify the correctness and effectiveness of the obtained results.
Microfluidic Platforms for the Isolation and Detection of Exosomes: A Brief Review
Authors: Raju Duraichelvan , Bathini Srinivas , Badilescu Simona , Ghosh Anirban , Packirisamy Muthukumaran
Published: May 2022

Extracellular vesicles (EVs) are a group of communication organelles enclosed by a phospholipid bilayer, secreted by all types of cells. The size of these vesicles ranges from 30 to 1000 nm, and they contain a myriad of compounds such as RNA, DNA, proteins, and lipids from their origin cells, offering a good source of biomarkers. Exosomes (30 to 100 nm) are a subset of EVs, and their importance in future medicine is beyond any doubt. However, the lack of efficient isolation and detection techniques hinders their practical applications as biomarkers. Versatile and cutting-edge platforms are required to detect and isolate exosomes selectively for further clinical analysis. This review paper focuses on lab-on-chip devices for capturing, detecting, and isolating extracellular vesicles. The first part of the paper discusses the main characteristics of different cell-derived vesicles, EV functions, and their clinical applications. In the second part, various microfluidic platforms suitable for the isolation and detection of exosomes are described, and their performance in terms of yield, sensitivity, and time of analysis is discussed.
Genotypic and Epidemiologic Profiles of Giardia duodenalis in Four Brazilian Biogeographic Regions
Authors: Calegar Deiviane A. , Nunes Beatriz C. , Monteiro Kerla J. L. , Bacelar Polyanna A. A. , Evangelista Brenda B. C. , Almeida Mayron M. , Silva Jurecir , Santos Jéssica P. , Boia Márcio N. , Jaeger Lauren H. , Carvalho-Costa Filipe A.
Published: May 2022

Human infections with gut protozoan parasites are neglected and not targeted by specific control initiatives, leading to a knowledge gap concerning their regional diversity and epidemiology. The present study aims to explore Giardia duodenalis genetic diversity and assess the epidemiologic scenario of subclinical infections in different Brazilian biogeographic regions. Cross-sectional surveys (n = 1334 subjects) were conducted in four municipalities in order to obtain fecal samples and socioenvironmental data. Microscopy of non-diarrheal feces and nucleotide sequencing of a β-giardin gene fragment were performed. From a total of 51 samples that could be sequenced, 27 (52.9%) β-giardin sequences were characterized as assemblage A and 24 (47.1%) as assemblage B. In the Amazon, assemblage B was the most frequently detected, predominantly BIII, and with two novel sub-assemblages. Assemblage A predominated in the extra-Amazon region, with five novel sub-assemblages. Prevalence reached 17.8% (64/360) in the Amazon, 8.8% (48/544) in the Atlantic Forest, 7.4% (22/299) in Cerrado and 2.3% (3/131) in the Semiarid. People living in poverty and extreme poverty presented significantly higher positivity rates. In conclusion, subclinical giardiasis is endemic in Brazilian communities in different biogeographic regions, presenting high genetic diversity and a heterogeneous genotypic distribution.
A Propidium Monoazide (PMAxx)-Droplet Digital PCR (ddPCR) for the Detection of Viable Burkholderia cepacia Complex in Nuclease-Free Water and Antiseptics
Authors: Daddy Gaoh Soumana , Kweon Ohgew , Lee Yong-Jin , Hussong David , Marasa Bernard , Ahn Youngbeom
Published: May 2022

Pharmaceutical products contaminated with Burkholderia cepacia complex (BCC) strains constitute a serious health issue for susceptible individuals. New detection methods to distinguish DNA from viable cells are required to ensure pharmaceutical product quality and safety. In this study, we have assessed a droplet digital PCR (ddPCR) with a variant propidium monoazide (PMAxx) for selective detection of live/dead BCC cells in autoclaved nuclease-free water after 365 days, in 0.001% chlorhexidine gluconate (CHX), and in 0.005% benzalkonium chloride (BZK) solutions after 184 days. Using 10 μM PMAxx and 5 min light exposure, a proportion of dead BCC was quantified by ddPCR. The detection limit of culture-based method was 104 CFU/mL, equivalent to 9.7 pg/μL for B. cenocepacia J2315, while that of ddPCR was 9.7 fg/μL. The true positive rate from nuclease-free water and CHX using PMAxx-ddPCR assay was 60.0% and 38.3%, respectively, compared to 85.0% and 74.6% without PMAxx (p < 0.05), respectively. However, in BZK-treated cells, no difference in the detection rate was observed between the ddPCR assay on samples treated with PMAxx (67.1%) and without PMAxx (63.3%). This study shows that the PMAxx-ddPCR assay provides a better tool for selective detection of live BCC cells in non-sterile pharmaceutical products.
Babesiosis in Southeastern, Central and Northeastern Europe: An Emerging and Re-Emerging Tick-Borne Disease of Humans and Animals
Authors: Bajer Anna , Beck Ana , Beck Relja , Behnke Jerzy M. , Dwużnik-Szarek Dorota , Eichenberger Ramon M. , Farkas Róbert , Fuehrer Hans-Peter , Heddergott Mike , Jokelainen Pikka , Leschnik Michael , Oborina Valentina , Paulauskas Algimantas , Radzijevskaja Jana , Ranka Renate , Schnyder Manuela , Springer Andrea , Strube Christina , Tolkacz Katarzyna , Walochnik Julia
Published: May 2022

There is now considerable evidence that in Europe, babesiosis is an emerging infectious disease, with some of the causative species spreading as a consequence of the increasing range of their tick vector hosts. In this review, we summarize both the historic records and recent findings on the occurrence and incidence of babesiosis in 20 European countries located in southeastern Europe (Bosnia and Herzegovina, Croatia, and Serbia), central Europe (Austria, the Czech Republic, Germany, Hungary, Luxembourg, Poland, Slovakia, Slovenia, and Switzerland), and northern and northeastern Europe (Lithuania, Latvia, Estonia, Iceland, Denmark, Finland, Sweden, and Norway), identified in humans and selected species of domesticated animals (cats, dogs, horses, and cattle). Recorded cases of human babesiosis are still rare, but their number is expected to rise in the coming years. This is because of the widespread and longer seasonal activity of Ixodes ricinus as a result of climate change and because of the more extensive use of better molecular diagnostic methods. Bovine babesiosis has a re-emerging potential because of the likely loss of herd immunity, while canine babesiosis is rapidly expanding in central and northeastern Europe, its occurrence correlating with the rapid, successful expansion of the ornate dog tick (Dermacentor reticulatus) populations in Europe. Taken together, our analysis of the available reports shows clear evidence of an increasing annual incidence of babesiosis across Europe in both humans and animals that is changing in line with similar increases in the incidence of other tick-borne diseases. This situation is of major concern, and we recommend more extensive and frequent, standardized monitoring using a “One Health” approach.
Metabolite Profiling in Green Microalgae with Varying Degrees of Desiccation Tolerance
Authors: Aigner Siegfried , Arc Erwann , Schletter Michael , Karsten Ulf , Holzinger Andreas , Kranner Ilse
Published: May 2022

Trebouxiophyceae are microalgae occupying even extreme environments such as polar regions or deserts, terrestrial or aquatic, and can occur free-living or as lichen photobionts. Yet, it is poorly understood how environmental factors shape their metabolism. Here, we report on responses to light and temperature, and metabolic adjustments to desiccation in Diplosphaera epiphytica, isolated from a lichen, and Edaphochlorella mirabilis, isolated from Tundra soil, assessed via growth and photosynthetic performance parameters. Metabolite profiling was conducted by GC–MS. A meta-analysis together with data from a terrestrial and an aquatic Chlorella vulgaris strain reflected elements of phylogenetic relationship, lifestyle, and relative desiccation tolerance of the four algal strains. For example, compatible solutes associated with desiccation tolerance were up-accumulated in D. epiphytica, but also sugars and sugar alcohols typically produced by lichen photobionts. The aquatic C. vulgaris, the most desiccation-sensitive strain, showed the greatest variation in metabolite accumulation after desiccation and rehydration, whereas the most desiccation-tolerant strain, D. epiphytica, showed the least, suggesting that it has a more efficient constitutive protection from desiccation and/or that desiccation disturbed the metabolic steady-state less than in the other three strains. The authors hope that this study will stimulate more research into desiccation tolerance mechanisms in these under-investigated microorganisms.
Comparative Evaluation of Three Immunoassays for the Simultaneous Detection of Clostridioides difficile Glutamate Dehydrogenase and Toxin A/B
Authors: Kim Namsu , Lee Seung Yeob , Park Joonhong , Lee Jaehyeon
Published: May 2022

Background: In the medical laboratory, a step-by-step workflow for Clostridioides difficile infection (CDI) detection using glutamate dehydrogenase (GDH) and toxin A/B assays for initial screening, along with a nucleic acid amplification test (NAAT), has been recommended recently. In this study, we evaluated these three immunoassays for the simultaneous detection of GDH and Clostridioides difficile (CD) toxin A/B. Methods: A total of 304 stool samples were tested for the presence of GDH antigen and CD toxin A/B using VIDAS C. difficile GDH and toxin A/B (CDAB), RIDASCREEN C. difficile GDH and toxin A/B (RIDA), and C. DIFF QUIK CHEK COMPLETE according to the manufacturers’ recommendations. As complementary reference methods for GDH and toxin A/B detection in the three immunoassays, CD cultures using ChromID C. difficile agar and the Xpert C. difficile assay, respectively, were tested. Results: All three GDH assays showed overall substantial agreement with the CD culture. All three toxin A/B assays showed overall moderate agreement with the Xpert C. difficile assay. In comparison with consensus results, VIDAS GDH and QCC GDH showed almost perfect agreement, whereas RIDA GDH showed inferior but substantial agreement. All three toxin A/B assays showed almost perfect agreement. Conclusions: Since the QCC GDH and toxin A/B assay is relatively more sensitive and specific than the other two immunoassays for discriminating toxigenic or non-toxigenic CDI, QCC is very helpful for the simultaneous identification of GDH and CD toxin A/B in the initial step of the two-round workflow for diagnosing CDI.
Comprehensive ADM1 Extensions to Tackle Some Operational and Metabolic Aspects in Anaerobic Digestion
Authors: Donoso-Bravo Andrés , Sadino-Riquelme María Constanza , Valdebenito-Rolack Emky , Paulet David , Gómez Daniel , Hansen Felipe
Published: May 2022

Modelling in anaerobic digestion will play a crucial role as a tool for smart monitoring and supervision of the process performance and stability. By far, the Anaerobic Digestion Model No. 1 (ADM1) has been the most recognized and exploited model to represent this process. This study aims to propose simple extensions for the ADM1 model to tackle some overlooked operational and metabolic aspects. Extensions for the discontinuous feeding process, the reduction of the active working volume, the transport of the soluble compound from the bulk to the cell interior, and biomass acclimation are presented in this study. The model extensions are included by a change in the mass balance of the process in batch and continuous operation, the incorporation of a transfer equation governed by the gradient between the extra- and intra- cellular concentration, and a saturation-type function where the time has an explicit influence on the kinetic parameters, respectively. By adding minimal complexity to the existing ADM1, the incorporation of these phenomena may help to understand some underlying process issues that remain unexplained by the current model structure, broadening the scope of the model for control and monitoring industrial applications.
Domestic Environment and Gut Microbiota: Lessons from Pet Dogs
Authors: Hernandez Juan , Rhimi Soufien , Kriaa Aicha , Mariaule Vincent , Boudaya Houda , Drut Amandine , Jablaoui Amin , Mkaouar Héla , Saidi Amel , Biourge Vincent , Borgi Mohamed Ali , Rhimi Moez , Maguin Emmanuelle
Published: May 2022

Accumulating data show the involvement of intestinal microbiota in the development and maintenance of numerous diseases. Many environmental factors influence the composition and function of the gut microbiota. An animal model subjected to the same environmental constraints that will allow better characterization of the microbiota–host dialogue is awaited. The domestic dog has physiological, dietary and pathological characteristics similar to those of humans and shares the domestic environment and lifestyle of its owner. This review exposes how the domestication of dogs has brought them closer to humans based on their intrinsic and extrinsic similarities which were discerned through examining and comparing the current knowledge and data on the intestinal microbiota of humans and canines in the context of several spontaneous pathologies, including inflammatory bowel disease, obesity and diabetes mellitus.
Evaluation of a Luminometric Cell Counting System in Context of Antimicrobial Photodynamic Inactivation
Authors: Lehnig Moritz , Glass Sarah , Lippmann Norman , Ziganshyna Svitlana , Eulenburg Volker , Werdehausen Robert
Published: May 2022

Antimicrobial resistance belongs to the most demanding medical challenges, and antimicrobial photodynamic inactivation (aPDI) is considered a promising alternative to classical antibiotics. However, the pharmacologic characterization of novel compounds suitable for aPDI is a tedious and time-consuming task that usually requires preparation of bacterial cultures and counting of bacterial colonies. In this study, we established and utilized a luminescence-based microbial cell viability assay to analyze the aPDI effects of two porphyrin-based photosensitizers (TMPyP and THPTS) on several bacterial strains with antimicrobial resistance. We demonstrate that after adaptation of the protocol and initial calibration to every specific bacterial strain and photosensitizer, the luminometric method can be used to reliably quantify aPDI effects in most of the analyzed bacterial strains. The interference of photosensitizers with the luminometric readout and the bioluminescence of some bacterial strains were identified as possible confounders. Using this method, we could confirm the susceptibility of several bacterial strains to photodynamic treatment, including extensively drug-resistant pathogens (XDR). In contrast to the conventional culture-based determination of bacterial density, the luminometric assay allowed for a much more time-effective analysis of various treatment conditions. We recommend this luminometric method for high-throughput tasks requiring measurements of bacterial viability in the context of photodynamic treatment approaches.
Oral Bacteria and Their Antibiotic Susceptibilities in Taiwanese Venomous Snakes
Authors: Chuang Po-Chun , Lin Wen-Hao , Chen Yi-Chun , Chien Chun-Chih , Chiu I-Min , Tsai Tein-Shun
Published: May 2022

Wound infections after venomous snakebites are clinically important. Information regarding the nature and antibiotic susceptibilities of snake oral bacterial flora could support empiric antibiotic therapy. Wild venomous snakes were collected from southern Taiwan: a total of 30 each of Bungarus multicinctus, Naja atra, Protobothrops mucrosquamatus, and Trimeresurus stejnegeri; 3 Deinagkistrodon acutus; and 4 Daboia siamensis. The species and antibiotic susceptibilities of their oral bacteria were determined. Aerobic gram-negative bacteria, especially Pseudomonas aeruginosa and Proteus vulgaris, were the most abundant. Proteus vulgaris were more abundant in B. multicinctus, N. atra, and P. mucrosquamatus than in T. stejnegeri (40%, 43.3%, and 40% vs. 13.3%, respectively). The gram-negative species were less susceptible to first- and second-generation cephalosporins and ampicillin-sulbactam than to third-generation cephalosporins, fluoroquinolones, carbapenems, or piperacillin-tazobactam. The most abundant aerobic gram-positive species cultured was Enterococcus faecalis, which was more abundant in N. atra than in other snakes (p < 0.001) and was highly susceptible to ampicillin, high-level gentamicin, penicillin, teicoplanin, and vancomycin. Bacteroides fragilis and Clostridium species were the most common anaerobic bacteria. The anaerobic organisms were highly susceptible to metronidazole and piperacillin. As a reference for empiric antimicrobial therapy, third-generation cephalosporins, fluoroquinolones, carbapenems, or piperacillin-tazobactam can be initiated in venomous snakebites wound infections.
Identification, Biochemical Characterization, and Safety Attributes of Locally Isolated Lactobacillus fermentum from Bubalus bubalis (buffalo) Milk as a Probiotic
Authors: Abid Sana , Farid Arshad , Abid Rameesha , Rehman Mujeeb , Alsanie Walaa , Alhomrani Majid , Alamri Abdulhakeem , Asdaq Syed , Hefft Daniel , Saqib Saddam , Muzammal Muhammad , Morshedy Sabrin , Alruways Mashael , Ghazanfar Shakira
Published: May 2022

The demand of functional foods is on the rise, and researchers are trying to develop nutritious dairy products by using well-characterized strains of bacteria. In this study, we identified locally isolated strains of Lactobacillus fermentum from Bubalus bubalis (Nilli Ravi buffalo) milk and evaluated their potential as probiotics in food products like fermented milk. Fifteen Lactobacillus strains were initially isolated, and only four strains (NMCC-2, NMCC-14, NMCC-17, and NMCC-27) were examined for morphological and biochemical characterizations due to their ability of gas production in Durham tubes. Moreover, these strains were selected for further probiotic characterizations due to their extreme morphological resemblance with lactic acid bacteria for their antimicrobial activity, enzymatic potential, autoaggregation capability, hydrophobicity, and acid and bile tolerance. All selected isolates showed significant probiotic potential. However, NMCC-14 and NMCC-17 strains showed maximum probiotic potential. The isolates (NMCC-2, NMCC-14, NMCC-17, and NMCC-27) were identified as Lactobacillus fermentum utilizing 16S rRNA gene sequencing. The in vivo safety study of NMCC-14 (dose: 1010 CFU/day/mice; 21 days, orally) showed no histological dysfunctions in a mouse model. Pathogenic bacterial enzymes reduced the beneficial bacterial load in the host gastrointestinal tract. These results suggest that the NMCC-14 strain is safe and can be potentially used as a probiotic. Moreover, fermented milk was prepared by using the NMCC-14 strain. The results revealed that NMCC-14 strain-based fermented milk had significantly (p < 0.05) higher protein content (4.4 ± 0.06), water-holding capacity (WHC), and dynamic viscosity as compared to non-fermented milk. The results suggest that L. fermentum NMCC-14 is safe and nontoxic; hence, it can be a beneficial supplement to be used for the development of dairy products to be subjected to further clinical testing.