bluuebunny/crossref_metadata_2025 huggingface dataset:
Will People Accept a Third Booster Dose of the COVID-19 Vaccine? A Cross-Sectional Study in China
Authors: Sun Yufang , Dai Hang , Wang Ping , Zhang Xiaodong , Cui Dongliang , Huang Yongping , Zhang Jimei , Xiang Tao
Published: July 2022

Objective

The coronavirus disease 2019 (COVID-19) vaccines are considered to be an effective way to prevent the spread of the infection. Our previous study has shown that about 75% of healthcare workers (HCWs) in China were willing to receive the vaccine when it became available. Here, we examined the acceptance of a third booster dose among Chinese people and identified the influencing factors.

Methods

A cross-sectional online survey was conducted and the snowball sampling method was utilized. An online questionnaire was provided to all the participants in the form of a quick response (QR) code. The questionnaire included general demographic information, views on vaccines, the General Health Questionnaire-12 (GHQ-12), and the Depression, Anxiety, and Stress Scale-21 (DASS-21). The univariate analysis was done between all the variables and our dependent variable. Then, we used the multivariate logistic regression model to examine the influencing factors of the third booster dose acceptance.

Results

We collected 1,062 complete answers. Of these, 90.39% (n = 960) declared that they would accept the booster dose. Knowing more about the vaccine and recognizing the efficacy of vaccines were significantly associated with greater acceptance of the booster dose. People willing to take the booster dose had better psychological health. A belief that the booster dose could prevent severe infection caused by COVID-19 and enhance the effectiveness of the first two doses were the main contributing factors to vaccine acceptance. Vaccine hesitancy was mainly due to a low perceived risk of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and rapid mutation of SARS-CoV-2.

Conclusion

This study revealed that Chinese people were very receptive to the third booster dose, which is an inspiring result. More positive attitudes regarding COVID-19 vaccination were supported by its efficacy and few side effects.
“We Should Be Taught Self-Respect, Self-Confidence and Self-Love”: Youth Perspectives of Adult Influences on Their Sexuality and Relationships Among South African Adolescents Living With HIV
Authors: Bergam Scarlett , Kuo Caroline , Atujuna Millicent , Pellowski Jennifer A. , Mtukushe Bulelwa , Ndevu-Qwabe Nontembeko , Matiwane Mluleki , Rencken Camerin A. , Belsky Mikaela , Hoare Jacqueline , Bekker Linda-Gail , Harrison Abigail D.
Published: July 2022

Introduction

Of the 1.75 million adolescents aged 10–19 years living with HIV globally, 84% reside in sub-Saharan Africa. This problem is most acute in South Africa, where there are 720,000 adolescents living with HIV (ALHIV). ALHIV navigate the same challenges as other adolescents—such as puberty and first relationships—as well as challenges specific to their HIV-status—including stigma, disclosure, and concerns about HIV transmission. This dual burden calls for tailored sexual and reproductive health (SRH) programs. Here, we qualitatively explore the reflections of South African ALHIV on SRH education, communication, and discussion provided by adults in schools, clinics, and the home related to their unique SRH needs.

Methods

This paper reports on qualitative data from a mixed-methods study to inform interventions that meet the SRH needs of ALHIV. In-depth interviews (N = 20) were conducted with ALHIV recruited from two clinics in Cape Town, South Africa. Nine males and 11 females aged 16–19 participated in semi-structured in-depth interviews to discuss their sexual health as ALHIV. The interview guide explored 1) perceived SRH needs; 2) healthy living with HIV; 3) future goals; 4) intimate relationships; 5) psychosocial challenges; and 6) preferred interventions. Data were thematically applied to an iteratively-developed codebook and analyzed by the cross-cultural research team using NVivo 12.

Results

These qualitative data reveal the pressing needs among ALHIV for open communication and accurate information about sexuality and HIV, given the risk to themselves and their partners as they enter intimate relationships. Three themes emerged from the data: 1) Intergenerational pressures coming from caregivers, clinicians, and teachers often stigmatize the sexual heath behaviors of ALHIV; 2) When present, open intergenerational communication can provide ALHIV with crucial information, resources, and social support that supports healthy decisions, and 3) ALHIV offer specific ideas about how adults can support their decision-making in the transition to adulthood.

Conclusions

Findings highlight adolescents' recommendations for programs involving open communication, stigma-reduction around sexuality, and support from both peer and adult mentors. This study lays the foundation for strategies to improve intergenerational communication about sexual health to promote positive approaches to sexuality for ALHIV.
Italian Legal Euthanasia: Unconstitutionality of the Referendum and Analysis of the “Italian” Problem
Authors: Marrone Maricla , Berardi Pietro , Solarino Biagio , Ferorelli Davide , Corradi Serena , Silvestre Maria , De Luca Benedetta Pia , Stellacci Alessandra , Dell'Erba Alessandro
Published: July 2022

The term “euthanasia” refers to medical interventions that involve the direct administration of a lethal drug to the patient who requests it and meets certain requirements. Currently in Italy euthanasia constitutes a crime and falls within the hypotheses foreseen and punished by Article 579 (Murder of consenting person) or by article 580 (Instigation or aid to suicide) of the penal code. On the contrary, medically assisted suicide in some cases and the suspension of treatment constitute an inviolable right pursuant to art. Thirty two of the Constitution and Law 219/2017. Thanks to the sentence 242/2019 of the Constitutional Court, in Italy it is instead possible to request medically assisted suicide, that is, the indirect help of a doctor to die. There are four conditions required: whoever requests it must be fully capable of understanding and willing, must have an irreversible pathology that is the bearer of severe physical or mental disease, and must survive thanks to life-saving treatments. The Italian referendum “Free until the end” aims to introduce legal euthanasia through the partial repeal of art. 579 c.p. which punishes the murder of the consenting party. The authors analyze the reasons for the referendum in the light of the Italian and European scenario, analyzing the first Italian case of assisted suicide immediately after the referendum which inevitably becomes a starting point for ethical and medico-legal reflection on the issue. On 02.15.2022 the Italian Constitutional Court declared the Referendum on Legal Euthanasia inadmissible.
Hip Torque Is a Mechanistic Link Between Sprint Acceleration and Maximum Velocity Performance: A Theoretical Perspective
Authors: Clark Kenneth P. , Ryan Laurence J.
Published: July 2022

Sprinting performance is critical for a variety of sports and competitive activities. Prior research has demonstrated correlations between the limits of initial acceleration and maximum velocity for athletes of different sprinting abilities. Our perspective is that hip torque is a mechanistic link between these performance limits. A theoretical framework is presented here that provides estimates of sprint acceleration capability based on thigh angular acceleration and hip torque during the swing phase while running at maximum velocity. Performance limits were calculated using basic anthropometric values (body mass and leg length) and maximum velocity kinematic values (contact time, thigh range of motion, and stride frequency) from previously published sprint data. The proposed framework provides a mechanistic link between maximum acceleration and maximum velocity, and also explains why time constant values (τ, ratio of the velocity limit to acceleration limit) for sprint performance curves are generally close to one-second even for athletes with vastly different sprinting abilities. This perspective suggests that specific training protocols targeted to improve thigh angular acceleration and hip torque capability will benefit both acceleration and maximum velocity phases of a sprint.
The Role of Amnioreduction in Emergency Cervical Cerclage with Bulging Membranes: A Retrospective Comparative Study
Authors: Zhang Yuan , Wang Qingqing , Tan Zhangmin , Zhou Jin , Zhang Peizhen , Hou Hongying , Yin Yuzhu , Han Zhenyan
Published: July 2022

The purpose of this study was to investigate the role of amnioreduction in patients who underwent emergency cervical cerclage (ECC) with bulging membranes during the second trimester. This retrospective comparative study included 46 singleton pregnant women who had cervical dilation at least 1 cm with bulging membranes beyond the external cervical os and underwent ECC at the Third Affiliated Hospital of Sun Yat-sen University between December 2016 and December 2021. Cases were categorized as amnioreduction group (n = 16) and non-amnioreduction group (n = 30) according to whether amnioreduction was performed prior to ECC. The gestational age and cervical dilation at cerclage, operative time, prolongation of pregnancy, and outcomes of pregnancy were compared between the two groups. All 46 patients underwent successful ECC excepted one case with intraoperative rupture of membrane in non-amnioreduction group. In the amnioreduction group, the cervical dilation at cerclage was larger than that in the non-amnioreduction group (4.5 ± 2.2 vs. 2.2 ± 1.2 cm, P < 0.001), and had more patients with cervical dilation ≥4 cm (50.0% vs. 10.0%, P = 0.004). However, the gestational age at cerclage, operative time, prolongation of pregnancy, gestational age at delivery were not significantly different between the two groups (22.9 ± 2.8 vs. 22.9 ± 3.2 weeks, 31.1 ± 9.2 vs. 27.9 ± 11.4 min, 21.3 ± 21.5 vs. 38.7 ± 40.2 days, 25.9 ± 4.5 vs. 28.4 ± 6.1 weeks; P > 0.05). The rates of delivery ≥28 weeks, ≥32 weeks, and live birth were 20.0% vs. 80.0%, 12.5% vs. 26.7%, 56.3% vs. 66.7% (P > 0.05) in amnioreduction group and non-amnioreduction group, respectively. In conclusion, even in cases with larger cervical dilation, the application of amnioreduction with ECC is possible to get the acceptable pregnancy outcomes. These findings suggested that amnioreduction may be a safe and feasible option to be applied before ECC, especially for those with advanced cervical dilation and bulging membranes.
Electrical Characteristics of Photovoltaic Cell in Solar-Powered Aircraft During Cruise
Authors: Li Peimiao , Wang Hui , Chang Min , Bai Junqiang
Published: July 2022

Aiming to study the electrical characteristics of photovoltaic cells during the flight of solar-powered unmanned aerial vehicles, this work combines a photovoltaic cell equivalent circuit model and a thermodynamic model. The influence of wing surface temperature and its influencing factor-solar radiation is of primary concern. A solar radiation model is established to explore the impact of solar irradiance on temperature and photovoltaic cell output. Atmospheric temperature and four basic parameters of photovoltaic cell, including open-circuit voltage, short-circuit current, voltage, and current at maximum power point under standard conditions are treated as input parameters. The surface temperature, the variation of output voltage, current, and power are studied with the altitude changing from 0 to 35 km and time from 0 to 24 h in spring equinoxes. Results find that with the increase in altitude, the surface temperature of the photovoltaic cell decreases first and then increases. The voltage of the photovoltaic cell decreases as the temperature increases, and the voltage-time curve varies at altitudes below 25 km and above 30 km. The peak power is available at an altitude between 15 and 20 km. The above findings can be applied to study energy generations and flows of solar-powered vehicles.
Effects of Different Roughages on Growth Performance, Nutrient Digestibility, Ruminal Fermentation, and Microbial Community in Weaned Holstein Calves
Authors: Li Jichao , Lian Hongxia , Zheng Airong , Zhang Jiangfan , Dai Pengfei , Niu Yan , Gao Tengyun , Li Ming , Zhang Liyang , Fu Tong
Published: July 2022

This study aimed to assess the effects of feeding with different forage sources and starter concentrations on growth performance, nutrient digestibility, ruminal fermentation, and the microbial community in weaned Holstein calves. A total of 54 Holstein calves (body weight (BW) = 77.50 ± 5.07 kg; age = 70 ± 2.54 days) were assigned to 1 of 3 treatment groups (n = 18/group) that were offered diets with different forages: (1) peanut vine (PV), (2) oat hay (OH), or (3) an alfalfa hay + oat hay combination (alfalfa hay:oat hay =1:1, AO). Starter and forage intakes were recorded daily, while BW and growth parameters were assessed at 15-day intervals. The apparent digestibility of nutrients was determined. Ruminal fluid samples were collected and used to detect relevant indicators. A difference was observed for the forage × age interaction for all feed, nutrient intake, BW, ADG, and body structure parameters (P < 0.05). The final BW, average daily feed intake (ADFI), and average daily gain of the PV calves were higher than those of calves from the other groups (P < 0.05). The ruminal propionate concentration evidently increased in calves of the AO group (P < 0.05). The abundances of Rikenellaceae_RC9_gut_group and Shuttleworthia showed distinct responses to feeding with different forages (P < 0.05) at the genus level. The relative abundance of Shuttleworthia was negatively related to rumen pH and acid detergent fiber digestibility (P < 0.05) and strongly positively related to propionate concentration (P < 0.01). A positive correlation was found between Ruminococcus_1 abundance and butyrate concentration and neutral detergent fiber digestibility (P < 0.05). The relative abundances of Succiniclasticum and Prevotella_7 were negatively related to butyrate concentration (P < 0.05). In conclusion, there was an interaction between the factors (forage × age). The peanut vine used as a forage source promoted a higher starter concentrate intake compared to other diets and increased with the calves' age. The growth performance and rumen bacterial community of the calves were further improved. These results indicate that peanut vine can be used as the main source of forage in the diets of weaned calves.
Tolerability and Effects of 2-Aminoethyl Dihydrogen Phosphate in Dogs With Mast Cell Tumors
Authors: Januário Eric Vieira , Melo Samanta Rios , Maria Durvanei Augusto , Lorigados Carla Aparecida Batista , Ambrósio Aline Magalhães , Kogika Marcia Mery , Cogliati Bruno , Shimozako Helio Junji , Matera Julia Maria
Published: July 2022

Canine mast cell tumor is a malignant neoplasm, and a gold standard treatment remains to be determined despite the proposed chemotherapies or other therapies in dogs. This study aimed to determine therapeutic, adverse effects and toxicity, tumor-free, and overall survival times of 10 dogs with surgically excised mast cell tumors evaluated by histopathological/immunohistochemistry and treated with four weekly intravenous administrations of 2-Aminoethyl Dihydrogen Phosphate (70 mg/kg) as adjuvant therapy. No adverse events were noted. Laboratory changes were limited (p < 0.05) in red blood cell, hemoglobin, and platelet counts. Mean tumor-free and overall survival were 599.1 ± 469 and 755.5 ± 423.5 days, respectively. In conclusion, 2-Aminoethyl Dihydrogen Phosphate administration was safe in dogs. However, 2-Aminoethyl Dihydrogen Phosphate was not sufficiently effective to prevent a recurrence, new tumor, or metastasis of canine mast cell tumors with poor immunohistochemical prognostic factors.
Camel Proteins and Enzymes: A Growing Resource for Functional Evolution and Environmental Adaptation
Authors: Kandeel Mahmoud , Al-Taher Abdulla , Venugopala Katharigatta N. , Marzok Mohamed , Morsy Mohamed , Nagaraja Sreeharsha
Published: July 2022

In less agroecological parts of the Asian, Arabian, and African deserts, Camelus dromedarius play an important role in human survival. For many years, camels have been employed as a source of food, a tool of transportation, and a means of defense. They are becoming increasingly important as viable livestock animals in many desert climates. With the help of camel genetics, genomics and proteomics known so far, this review article will summarize camel enzymes and proteins, which allow them to thrive under varied harsh environmental situations. An in-depth study of the dromedary genome revealed the existence of protein-coding and fast-developing genes that govern a variety of metabolic responses including lipid and protein metabolism, glucoamylase, flavin-containing monooxygenase and guanidinoacetate methyltransferase are other metabolic enzymes found in the small intestine, liver, pancreas, and spleen. In addition, we will discuss the handling of common medications by camel liver cytochrome p 450, which are different from human enzymes. Moreover, camels developed several paths to get optimum levels of trace elements like copper, zinc, selenium, etc., which have key importance in their body for normal regulation of metabolic events. Insulin tolerance, carbohydrate and energy metabolism, xenobiotics metabolizing enzymes, vimentin functions, behavior during the rutting season, resistance to starvation and changes in blood composition and resistance to water loss were among the attractive aspects of camel enzymes and proteins peculiarities in the camels. Resolving the enigma of the method of adaptation and the molecular processes linked with camel life is still a developing repository full of mysteries that need additional exploration.
Impact of Sub-MIC Eugenol on Klebsiella pneumoniae Biofilm Formation via Upregulation of rcsB
Authors: Elken Emad Mohammed , Tan Zi-ning , Wang Qian , Jiang Xiu-yun , Wang Yu , Wang Yi-ming , Ma Hong-xia
Published: July 2022

The Rcs phosphorelay system is present in many members of the Enterobacteriaceae. The aim of this study was to illustrate the possible mechanisms of eugenol on ultimate targets of Klebsiella pneumoniae (K. pneumoniae) Rcs phosphorelay, rcsB, and impact on biofilm formation. The minimum inhibitory concentration (MIC) of eugenol against K. pneumoniae KP1 and KP1 ΔrcsB strain was determined using the 2-fold micro-dilution method. Biofilm was measured by crystal violet staining. Transcriptome sequencing was performed to investigate sub-MIC eugenol on K. pneumoniae, and gene expression at mRNA level was analyzed by RT-qPCR. In vitro biofilm formation test and molecular docking were used to evaluate the effect of eugenol and to predict potential interactions with RcsB. MicroScale Thermophoresis (MST) was conducted for further validation. MIC of eugenol against K. pneumoniae KP1 and KP1 ΔrcsB strain was both 200 μg/ml. Transcriptome sequencing and RT-qPCR results indicated that rpmg, degP, rnpA, and dapD were downregulated, while rcsB, rcsD, rcsA, yiaG, and yiaD were upregulated in the eugenol-treated group. ΔrcsB exhibited a weakened biofilm formation capacity. Additional isopropyl-β-d-thiogalactoside (IPTG) hinders biofilm formation, while sub-MIC eugenol could promote biofilm formation greatly. Docking analysis revealed that eugenol forms more hydrophobic bonds than hydrogen bonds. MST assay also showed a weak binding affinity between eugenol and RcsB. These results provide significant evidence that rcsB plays a key role in K. pneumoniae biofilm formation. Sub-MIC eugenol facilitates biofilm formation to a large extent instead of inhibiting it. Our findings reveal the potential risk of natural anti-biofilm ingredients at sub-MIC to treat drug-resistance bacteria.
An Analysis of the Determinants of Irrigation Farmworkers’ Food Security Status: A Case of Tshiombo Irrigation Scheme, South Africa
Authors: Mudzielwana Rudzani , Mafongoya Paramu , Mudhara Maxwell
Published: July 2022

Food insecurity is a comprehensive challenge. Food, being one of the most basic human needs, has become one of the most important concerns in the world, as more people are living in poverty and are vulnerable to food insecurity. Food insecurity levels vary across sectors, meaning that policy recommendations to address the problem have to be in specific contexts. Farmworkers in irrigation schemes are a sub-group that has received little attention in research regarding food security outcomes. This paper provides evidence of a study that was carried out to analyze food security among irrigation scheme farmworkers who either rent or do not rent irrigation plots from their employers in the Tshiombo Irrigation Scheme, Limpopo Province. Data were collected from 191 randomly selected farmworkers. The Household Food Insecurity Access Scale (HFIAS) was utilized to determine the extent of food security among the irrigation scheme farmworkers. Data were analyzed using the ordered probit model. Among the variables considered in the model, land size (p < 0.05), land leasing (p < 0.01), total household expenditure (p < 0.05), and food stored by farmworkers (p < 0.1) were found to significantly influence irrigation farmworkers’ food security status. These findings suggest that policymakers should design policies that encourage stakeholders from NGOs, and private and public sectors to train and provide resources that will enable and develop livelihood skills among farmworkers.
Identification of Male and Female Parents for Hybrid Rice Seed Production Using UAV-Based Multispectral Imagery
Authors: Liu Hanchao , Qi Yuan , Xiao Wenwei , Tian Haoxin , Zhao Dehua , Zhang Ke , Xiao Junqi , Lu Xiaoyang , Lan Yubin , Zhang Yali
Published: July 2022

Identifying and extracting male and female parent of hybrid rice and then accurately judging the spikelet flowering of male parents is the basis of hybrid rice pollination. Currently, male parent flowering information extraction for hybrid rice is basically obtained by manual recognition. In this study, remote sensing images of parental rice fields were obtained with a multispectral camera carried by a UAV (Umanned Aerial Vehicle). Six kinds of visible light vegetation indices and four kinds of multispectral vegetation indices, together with two classification methods, pixel-based supervised classification and sample-based object-oriented classification, were applied to identify the male and female parents of hybrid rice, after which the accuracies of the methods were compared. The results showed that the visible vegetation index had a better effect in pixel-based supervised classification. The kappa coefficient of ExGR (Excess Green minus Excess Red index) classification was 0.9256 and the total accuracy was 0.9552. The extraction accuracy was higher than that of the other vegetation indices and object-oriented classification. In pixel-based supervised classification, the maximum likelihood method achieved the highest identification accuracy and shortest calculation time. Taking the remote sensing images obtained with a UAV as a data source, maximum likelihood supervised classification based on ExGR index can more effectively and quickly identify the field information of male and female parents of hybrid rice so as to provide a reference for determining optimal pollination timing for hybrid rice in large-scale seed production farms.
Comparison of Field Performance of Different Driving Systems and Forward Speed Measuring Methods for a Wet Direct Seeder of Rice
Authors: Wang Yunxia , Zhang Wenyi , Qi Bing , Xia Qianqian
Published: July 2022

Research on precision seeders operated in the dry field shows that an electric driving system used for a seed meter can significantly improve the planting uniformity. The measuring method for forward speed greatly affects the control accuracy of the electric driving system. However, the performance of an electric drive system for precision seeders operating in wet fields, such as paddy, is still unclear. The operating conditions of dry and wet fields are quite different, and it is necessary to study the effects of an electric driving system and corresponding speed measuring methods on a wet-field operating seeder. In this paper, field experiments were carried out by using a wet direct planter of rice under three driving methods for seed meter, including classic mechanical driving system (MDS), electric driving system with speed acquiring from an encoder (EDSE), and electric driving system with speed acquiring from the global positioning system (EDSG), at forward speeds of 4.62, 5.81, and 7.23 km·h−1. The seeding uniformity (seeding rates, coefficient of variations) and the slippage (slippage rate, broken strips) were investigated. Results showed that the EDSG exhibited more qualified operation performance compared with the MDS and the EDSE. For both the MDS and EDSE, the seeding rates significantly decreased and deviated from the desired value as the forward speed increased. The negative slippages of MDS and EDSE were 3.47–31.72% and 2.13–7.7%, respectively, and they significantly increased when the forward speeds increased from 4.62 to 7.23 km·h−1. Analysis of the seeding uniformity and slippage presented that the EDSG exhibited a more qualified operation and is recommended for the wet direct seeder of rice.
The Association between Biofilm Formation and Antimicrobial Resistance with Possible Ingenious Bio-Remedial Approaches
Authors: Dutt Yogesh , Dhiman Ruby , Singh Tanya , Vibhuti Arpana , Gupta Archana , Pandey Ramendra Pati , Raj V. Samuel , Chang Chung-Ming , Priyadarshini Anjali
Published: July 2022

Biofilm has garnered a lot of interest due to concerns in various sectors such as public health, medicine, and the pharmaceutical industry. Biofilm-producing bacteria show a remarkable drug resistance capability, leading to an increase in morbidity and mortality. This results in enormous economic pressure on the healthcare sector. The development of biofilms is a complex phenomenon governed by multiple factors. Several attempts have been made to unravel the events of biofilm formation; and, such efforts have provided insights into the mechanisms to target for the therapy. Owing to the fact that the biofilm-state makes the bacterial pathogens significantly resistant to antibiotics, targeting pathogens within biofilm is indeed a lucrative prospect. The available drugs can be repurposed to eradicate the pathogen, and as a result, ease the antimicrobial treatment burden. Biofilm formers and their infections have also been found in plants, livestock, and humans. The advent of novel strategies such as bioinformatics tools in treating, as well as preventing, biofilm formation has gained a great deal of attention. Development of newfangled anti-biofilm agents, such as silver nanoparticles, may be accomplished through omics approaches such as transcriptomics, metabolomics, and proteomics. Nanoparticles’ anti-biofilm properties could help to reduce antimicrobial resistance (AMR). This approach may also be integrated for a better understanding of biofilm biology, guided by mechanistic understanding, virtual screening, and machine learning in silico techniques for discovering small molecules in order to inhibit key biofilm regulators. This stimulated research is a rapidly growing field for applicable control measures to prevent biofilm formation. Therefore, the current article discusses the current understanding of biofilm formation, antibiotic resistance mechanisms in bacterial biofilm, and the novel therapeutic strategies to combat biofilm-mediated infections.
Investigating Dual-Species Candida auris and Staphylococcal Biofilm Antiseptic Challenge
Authors: Gülmez Dolunay , Brown Jason L. , Butcher Mark C. , Delaney Christopher , Kean Ryan , Ramage Gordon , Short Bryn
Published: July 2022

Candida auris can persistently colonize human skin, alongside a diverse bacterial microbiome. In this study we aimed to investigate the efficacy of antiseptic activities on dual-species interkingdom biofilms containing staphylococci to determine if antiseptic tolerance was negatively impacted by dual-species biofilms. Chlorhexidine, povidone iodine, and hydrogen peroxide (H2O2), were able to significantly reduce biofilm viable cell counts following exposure at 2%, 10%, and 3%, respectively. Notably, H2O2-treated biofilms were able to significantly recover and considerably repopulate following treatment. Fortunately, inter-kingdom interactions in dual-species biofilms of C. auris and staphylococci did not increase the tolerance of C. auris against antiseptics in vitro. These data indicate mixed infections are manageable with chlorhexidine and povidone iodine, but caution should be exercised in the consideration of H2O2.
Antibiofilm Activity of Omega-3 Fatty Acids and Its Influence on the Expression of Biofilm Formation Genes on Staphylococcus aureus
Authors: Spiegel Christopher , Steixner Stephan Josef Maria , Coraça-Huber Débora C.
Published: July 2022

Background: Currently, 1–2% of all prosthetic joint surgeries are followed by an infection. These infections cause approximately 4% of deaths in the first year after surgery, while the 5-year mortality rate is up to 21%. Prosthetic joint infections are mainly caused by Staphylococcus aureus or Staphylococcus epidermis strains. Both species share the capability of biofilm formation and methicillin resistance. The formation of biofilm helps bacterial cells to withstand critical environmental conditions. Due to their tolerance against antibacterial substances, biofilms are a significant problem in modern medicine. Alternatives for the use of methicillin as a therapeutic are not yet widespread. The use of omega-3 fatty acids, such as docosahexaenoic acid, may help against prosthetic joint infections and lower mortality rates. The aim of this study is to evaluate if docosahexaenoic acid offers a safe anti-biofilm activity against Staphylococcus aureus and MRSA without enhancing icaADBC-dependent biofilm formation or additional stress responses, therefore enhancing antibiotic tolerance and resistance. Methods: In this study, we examined the gene expression of biofilm-associated genes and regulators. We performed RT-qPCR after RNA extraction of Staphylococcus aureus ATCC 29213 and one clinical MRSA strain. We compared gene expression of icaADBC, SarA, SigB, and agrAC under the influence of 1.25 mg /L and 0.625 mg/L of docosahexaenoic acid to their controls. Results: We found a higher expression of regulatory genes such as SarA, SigB, agrA, and agrC at 1.25 mg/L of docosahexaenoic acid in ATCC 29213 and a lower increase in gene expression levels in clinical MRSA isolates. icaADBC was not affected in both strains at both concentration levels by docosahexaenoic acid. Conclusions: Docosahexaenoic acid does not enhance icaADBC-dependent biofilm formation while still reducing bacterial CFU in biofilms. Docosahexaenoic acid can be considered an option as a therapeutic substance against biofilm formation and may be a good alternative in reducing the risk of MRSA formation.
Therapeutic Intervention in Cancer by Isoliquiritigenin from Licorice: A Natural Antioxidant and Redox Regulator
Authors: Zhang Zhu , Yung Ken Kin-Lam , Ko Joshua Ka-Shun
Published: July 2022

Oxidative stress could lead to a variety of body dysfunctions, including neurodegeneration and cancer, which are closely associated with intracellular signal transducers such as reactive oxygen species (ROS). It has been suggested that ROS is the upstream regulator of autophagy, and that it provides a negative feedback regulation to remove oxidative damage. Defects in the ROS-autophagic redox homeostasis could lead to the increased production of ROS and the accumulation of damaged organelles that in turn promote metabolic reprogramming and induce tumorigenesis. One significant characteristic of pancreatic cancer is the reprogramming of cellular energy metabolism, which facilitates the rapid growth, invasiveness, and the survival of cancer cells. Thus, the rectification of metabolic dysfunction is essential in therapeutic cancer targeting. Isoliquiritigenin (ISL) is a chalcone obtained from the plant Glycyrrhiza glabra, which is a powdered root licorice that has been consumed for centuries in different regions of the world. ISL is known to be a natural antioxidant that possesses diversified functions, including redox regulation in cells. This review contains discussions on the herbal source, biological properties, and anticancer potential of ISL. This is the first time that the anticancer activities of ISL in pancreatic cancer has been elucidated, with a coverage of the involvement of antioxidation, metabolic redox regulation, and autophagy in pancreatic cancer development. Furthermore, some remarks on related compounds of the isoflavonoid biosynthetic pathway of ISL will also be discussed.
Development and Functional Analysis of Lithocarpus polystachyus (wall.) Rehd Black Tea
Authors: Liu Yuqian , Teng Teng , Ye Kun , Zhou Guolan , Yang Xiulong , Zhao De-Gang , Zhao Yichen
Published: July 2022

This study examined the development conditions and functional properties of a novel compound tea Lithocarpus polystachyus (wall.) Rehd (L. polystachyus, LPR) black tea (LPRBT). The compound tea was developed by fermentation using fresh leaves (Camellia sinensis cv. Qianmei 601) as the main raw material with LPR powder as an additive. Based on the single factor and orthogonal tests with sensory scores as indicators, a withered leaves–LPR powder mass ratio of 9:1 with a 6 h fermentation time was determined to be the production condition of LPRBT with a sensory score of 89.09. In addition, phlorizin content, anti-oxidation function, hypoglycemic function, and tumor suppressor effect of LPRBT were measured. The results demonstrated that LPRBT phlorizin content was significantly higher than apple. It also showed that the equivalent 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical clearance rate with Vitamin C (Vc) and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical clearance rate was 81% of Vc. Both hydroxyl and superoxide anion radical clearance increased with the increase in LPRBT amount. LPRBT also showed a good inhibitory effect on α-glucosidase and α-amylase, indicating certain hypoglycemic activity. Moreover, it inhibited the growth of HeLa and A549 cancer cells showing tumor suppressor activity. This study provides a reference for the development and application of LPR food products.
Load Transfer Behavior and Failure Mechanism of Bird’s Nest Anchor Cable Anchoring Structure
Authors: Zhu Changxing , Zhao Weihao , Liu Xu
Published: July 2022

To research the internal load transfer behavior and failure mechanism of a bird’s nest anchor cable anchoring structure based on a pull-out test, a bond-slip failure model is established on the basis of statistical damage theory, and the distribution formula of shear stress at anchorage agent–rock interface is deduced. Combined with theoretical analysis, bird’s nest anchor cable pulling out test and particle flow code (PFC) numerical simulation test, as well as axial force distribution of the cable and shear stress distribution of its interface, help reveal its load transfer behavior and failure mechanism. Results show that: (1) The established bond-slip model can reflect the internal load transfer behavior and failure process of bird’s nest anchor cable anchorage structure. (2) The shear stress of the anchorage agent interface increases exponentially to the peak value and then decreases exponentially to the residual strength. The process is repeated at every location of the anchorage agent interface. The curve of the axial force and shear stress of the bird’s nest anchor cable is a negative exponential distribution with anchorage depth, and the maximum value occurs at the load end. (3) The crack of the anchorage agent interface extends from the load end to the other end and finally cuts through the whole interface. Rock mass generates radial cracks by the split effects of the bird’s nest. The failure mode is a combination of the debonding slip of the interface and the shear failure of the rock mass. The shear stress distribution and failure mode of the anchor structure are basically consistent according to laboratory tests and simulation tests, and PFC2D better reflects the internal load transfer behavior, failure mechanism, and failure process of the bird’s nest anchor cable under tensile loads.
Construction of Power Fault Knowledge Graph Based on Deep Learning
Authors: Liu Peishun , Tian Bing , Liu Xiaobao , Gu Shijing , Yan Li , Bullock Leon , Ma Chao , Liu Yin , Zhang Wenbin
Published: July 2022

A knowledge graph can structure heterogeneous knowledge in the field of power faults, construct the correlation between different pieces of knowledge, and solve the diversification, complexity, and island of fault data. There are many kinds of entities in power fault defect text, the relationship between entities is complex, and the data are often mixed with noise. It is necessary to research how to effectively mine the target data and separate the salient knowledge from the noise. Moreover, the traditional entity and relationship extraction methods used in the construction of a power fault knowledge graph cannot fully understand the text semantics, and the response accuracy is low. The Log system usually contains all kinds of information related to faults and a log analysis helps us collect fault information and perform association analysis. Therefore, a Bidirectional Sliced GRU with Gated Attention mechanism (BiSGRU-GA) model is proposed to detect the anomalous logs in the power system, this enriches the fault knowledge base and provides a good data resource for the construction of the knowledge graph. A new Bidirectional GRU with Gated Attention mechanism and Conditional Random Fields and a BERT input layer (BBiGRU-GA-CRF) model is proposed by introducing a BERT layer and Attention Mechanism into the Bidirectional GRU (BiGRU) model to more fully understand the context information of fault sentences and improve the accuracy of entity recognition of fault sentences. Aiming to solve the problems of large calculation cost and propagation error which occur in the traditional relationship extraction model, an improved Bidirectional Gated Recurrent Unit neural network with fewer parameters and the Gated Attention Mechanism (BiGRU-GA) model is proposed. This new model introduces an improved Gated Attention Mechanism to achieve better effects in relationship extraction. Compared with Bidirectional Long Short-Term Memory with Attention Mechanism (BiLSTM-Attention), the accuracy, recall, and F-measure of the model were improved by 1.79%, 13.83%, and 0.30% respectively, and the time cost is reduced by about 16%. The experimental results show that the BiGRU-GA model can capture local features, reduce the training time cost, and improve the model recognition effect.
Design of WPT System Based on Interleaved Boost Converter
Authors: Wu Jing , Zheng Weiyan , Jiang Yanping , Wang Yijie
Published: July 2022

With the improvement of technology, the demand for electrical power continues to deepen. Wireless Power Transfer (WPT) technology can transmit power without using physical media such as cables, and it has the advantages of electrical isolation, convenience, and safety. At present, the miniaturization of the secondary side is an emerging trend of WPT systems, which is analyzed in this paper. By introducing an interleaved boost converter in the front stage, the DC bus voltage of the primary side is increased, the loss of the primary side is reduced, and the system efficiency is improved. At the same time, the self-inductance of the primary and secondary sides of the loosely coupled transformer is designed to meet the stress requirements of passive devices and the realization of a closed-loop system. Finally, a WPT system with an input voltage of 100 V, an output voltage of 100 V and a transmission power of 500 W is built. The transmission distance of this system is 170 mm, with a lateral offset of 200 mm and a vertical offset of 100 mm. After the offset of the primary and secondary sides, the output voltage can be stabilized at 100 V, and the system efficiency can reach 90.1%, which proves the feasibility of the system efficiency improvement strategy and the effectiveness of the closed-loop control.
Photoionization of Atomic Systems Using the Random-Phase Approximation Including Relativistic Interactions
Authors: Deshmukh Pranawa C. , Manson Steven T.
Published: July 2022

Approximation methods are unavoidable in solving a many-electron problem. One of the most successful approximations is the random-phase approximation (RPA). Miron Amusia showed that it can be used successfully to describe atomic photoionization processes of many-electron atomic systems. In this article, the historical reasons behind the term “random-phase approximation” are revisited. A brief introduction to the relativistic RPA (RRPA) developed by Walter Johnson and colleagues is provided and some of its illustrative applications are presented.
RETRACTED: Shin, J.-H.; Jeong, C.-W. Zipper Is Necessary for Branching Morphogenesis of the Terminal Cells in the Drosophila melanogaster’s Tracheal System. Biology 2021, 10, 729
Authors: Shin Jong-Hyeon , Jeong Chan-Woo
Published: July 2022

The published article [...]
Understanding the Role of PIN Auxin Carrier Genes under Biotic and Abiotic Stresses in Olea europaea L.
Authors: Cardoso Hélia , Campos Catarina , Grzebelus Dariusz , Egas Conceição , Peixe Augusto
Published: July 2022

The PIN-FORMED (PIN) proteins represent the most important polar auxin transporters in plants. Here, we characterized the PIN gene family in two olive genotypes, the Olea europaea subsp. europaea var. sylvestris and the var. europaea (cv. ‘Farga’). Twelve and 17 PIN genes were identified for vars. sylvestris and europaea, respectively, being distributed across 6 subfamilies. Genes encoding canonical OePINs consist of six exons, while genes encoding non-canonical OePINs are composed of five exons, with implications at protein specificities and functionality. A copia-LTR retrotransposon located in intron 4 of OePIN2b of var. europaea and the exaptation of partial sequences of that element as exons of the OePIN2b of var. sylvestris reveals such kind of event as a driving force in the olive PIN evolution. RNA-seq data showed that members from the subfamilies 1, 2, and 3 responded to abiotic and biotic stress factors. Co-expression of OePINs with genes involved in stress signaling and oxidative stress homeostasis were identified. This study highlights the importance of PIN genes on stress responses, contributing for a holistic understanding of the role of auxins in plants.
Ecotourism Disturbance on an Endemic Endangered Primate in the Huangshan Man and the Biosphere Reserve of China: A Way to Move Forward
Authors: Li Wen-Bo , Yang Pei-Pei , Xia Dong-Po , Huffman Michael A. , Li Ming , Li Jin-Hua
Published: July 2022

The primary purpose of the Man and the Biosphere Program is the sustainable development of both the economy and nature conservation activities. Although the effectiveness of eco-tourism to reach this goal has been proposed, due to the lack of long-term monitoring data and a model species, there has been no obvious mechanism to evaluate the effectiveness of this policy. This study explored the effectiveness of the sustainable development policy of HMBR based on 30 years data of monitoring the Tibetan macaque, local human population, visitors, and annual ecotourism income in Huangshan by estimating species habitat suitability and the impact of ecotourism. The results showed increases in the income for the local human population, the number of visitors, and annual eco-tourism. Simultaneously, the reserve’s Tibetan macaque population size and suitable habitat areas increased. The macaques expanded their habitat to the low-altitude buffer zone (400–800 m), an area with lower eco-tourism disturbance. Scenic spots had a significant negative impact on habitat suitability (the substantially increased contributions of scenic spots from 0.71% to 32.88%). Our results and methods provide a suitable evaluation framework for monitoring the sustainable development and effectiveness of eco-tourism and wildlife conservation in Man and the Biosphere reserves.
Pathological Features and Neuroinflammatory Mechanisms of SARS-CoV-2 in the Brain and Potential Therapeutic Approaches
Authors: Sodagar Aisha , Javed Rasab , Tahir Hira , Razak Saiful Izwan Abd , Shakir Muhammad , Naeem Muhammad , Yusof Abdul Halim Abdul , Sagadevan Suresh , Hazafa Abu , Uddin Jalal , Khan Ajmal , Al-Harrasi Ahmed
Published: July 2022

The number of deaths has been increased due to COVID-19 infections and uncertain neurological complications associated with the central nervous system. Post-infections and neurological manifestations in neuronal tissues caused by COVID-19 are still unknown and there is a need to explore how brainstorming promoted congenital impairment, dementia, and Alzheimer’s disease. SARS-CoV-2 neuro-invasion studies in vivo are still rare, despite the fact that other beta-coronaviruses have shown similar properties. Neural (olfactory or vagal) and hematogenous (crossing the blood–brain barrier) pathways have been hypothesized in light of new evidence showing the existence of SARS-CoV-2 host cell entry receptors into the specific components of human nerve and vascular tissue. Spike proteins are the primary key and structural component of the COVID-19 that promotes the infection into brain cells. Neurological manifestations and serious neurodegeneration occur through the binding of spike proteins to ACE2 receptor. The emerging evidence reported that, due to the high rate in the immediate wake of viral infection, the olfactory bulb, thalamus, and brain stem are intensely infected through a trans-synaptic transfer of the virus. It also instructs the release of chemokines, cytokines, and inflammatory signals immensely to the blood–brain barrier and infects the astrocytes, which causes neuroinflammation and neuron death; and this induction of excessive inflammation and immune response developed in more neurodegeneration complications. The present review revealed the pathophysiological effects, molecular, and cellular mechanisms of possible entry routes into the brain, pathogenicity of autoantibodies and emerging immunotherapies against COVID-19.