bluuebunny/crossref_metadata_2025 huggingface dataset:
The Interplay of Cohesin and the Replisome at Processive and Stressed DNA Replication Forks
Authors: van Schie Janne J. M. , de Lange Job
Published: December 2021

The cohesin complex facilitates faithful chromosome segregation by pairing the sister chromatids after DNA replication until mitosis. In addition, cohesin contributes to proficient and error-free DNA replication. Replisome progression and establishment of sister chromatid cohesion are intimately intertwined processes. Here, we review how the key factors in DNA replication and cohesion establishment cooperate in unperturbed conditions and during DNA replication stress. We discuss the detailed molecular mechanisms of cohesin recruitment and the entrapment of replicated sister chromatids at the replisome, the subsequent stabilization of sister chromatid cohesion via SMC3 acetylation, as well as the role and regulation of cohesin in the response to DNA replication stress.
New Insights into Epigenetic Regulation of T Cell Differentiation
Authors: Dutta Avik , Venkataganesh Harini , Love Paul E.
Published: December 2021

Immature CD4− CD8− thymocytes progress through several developmental steps in the thymus, ultimately emerging as mature CD4+ (helper) or CD8+ (cytotoxic) T cells. Activation of naïve CD4+ and CD8+ T cells in the presence of specific cytokines results in the induction of transcriptional programs that result in their differentiation into effector or memory cells and in the case of CD4+ T cells, the adoption of distinct T-helper fates. Previous studies have shown that histone modification and DNA methylation play important roles in each of these events. More recently, the roles of specific epigenetic regulators in T cell differentiation have been clarified. The identification of the epigenetic modifications and modifiers that control mature T cell differentiation and specification has also provided further insights into how dysregulation of these processes can lead to cancer or autoimmune diseases. In this review, we summarize recent findings that have provided new insights into epigenetic regulation of T cell differentiation in both mice and humans.
Small Endogenous Ligands Modulation of Nerve Growth Factor Bioactivity: A Structural Biology Overview
Authors: Paoletti Francesca , Lamba Doriano
Published: December 2021

Experiments with cell cultures and animal models have provided solid support for the assumption that Nerve Growth Factor (NGF) plays a key role in the regulation of neuronal cell survival and death. Recently, endogenous ligands have been proposed as physiological modulators of NGF biological activity as part of this regulatory cascade. However, the structural and mechanistic determinants for NGF bioactivity remain to be elucidated. We recently unveiled, by an integrated structural biology approach, the ATP binding sites of NGF and investigated the effects on TrkA and p75NTR receptors binding. These results pinpoint ATP as a genuine endogenous modulator of NGF signaling, paving the way to the characterization of not-yet-identified chemical diverse endogenous biological active small molecules as novel modulators of NGF. The present review aims at providing an overview of the currently available 3D structures of NGF in complex with different small endogenous ligands, featuring the molecular footprints of the small molecules binding. This knowledge is essential for further understanding the functional role of small endogenous ligands in the modulation of neurotrophins signaling in physiological and pathological conditions and for better exploiting the therapeutic potentialities of NGF.
NPC1L1 Deficiency Suppresses Ileal Fibroblast Growth Factor 15 Expression and Increases Bile Acid Pool Size in High-Fat-Diet-Fed Mice
Authors: Jia Lin , Ma Yinyan , Haywood Jamie , Jiang Long , Xue Bingzhong , Shi Hang , Dawson Paul A. , Yu Liqing
Published: December 2021

Niemann–Pick C1-like 1 (NPC1L1) mediates intestinal uptake of dietary and biliary cholesterol and is the target of ezetimibe, a cholesterol absorption inhibitor used to treat hypercholesterolemia. Genetic deletion of NPC1L1 or ezetimibe treatment protects mice from high-fat diet (HFD)-induced obesity; however, the molecular mechanisms responsible for this therapeutic benefit remain unknown. A major metabolic fate of cholesterol is its conversion to bile acids. We found that NPC1L1 knockout (L1-KO) mice fed an HFD had increased energy expenditure, bile acid pool size, and fecal bile acid excretion rates. The elevated bile acid pool in the HFD-fed L1-KO mice was enriched with tauro-β-muricholic acid. These changes in the L1-KO mice were associated with reduced ileal mRNA expression of fibroblast growth factor 15 (FGF15) and increased hepatic mRNA expression of cholesterol 7α-hydroxylase (Cyp7A1) and mitochondrial sterol 27-hydroxylase (Cyp27A1). In addition, mRNA expression of the membrane bile acid receptor Takeda G protein-coupled receptor 5 (TGR5) and type 2 iodothyronine deiodinase (Dio2) were elevated in brown adipose tissue of L1-KO mice, which is known to promote energy expenditure. Thus, altered bile acid homeostasis and signaling may play a role in protecting L1-KO mice against HFD-induced obesity.
Cellular Senescence in Adrenocortical Biology and Its Disorders
Authors: Gao Xin , Li Faping , Liu Bin , Wang Yuxiong , Wang Yishu , Zhou Honglan
Published: December 2021

Cellular senescence is considered a physiological process along with aging and has recently been reported to be involved in the pathogenesis of many age-related disorders. Cellular senescence was first found in human fibroblasts and gradually explored in many other organs, including endocrine organs. The adrenal cortex is essential for the maintenance of blood volume, carbohydrate metabolism, reaction to stress and the development of sexual characteristics. Recently, the adrenal cortex was reported to harbor some obvious age-dependent features. For instance, the circulating levels of aldosterone and adrenal androgen gradually descend, whereas those of cortisol increase with aging. The detailed mechanisms have remained unknown, but cellular senescence was considered to play an essential role in age-related changes of the adrenal cortex. Recent studies have demonstrated that the senescent phenotype of zona glomerulosa (ZG) acts in association with reduced aldosterone production in both physiological and pathological aldosterone-producing cells, whereas senescent cortical-producing cells seemed not to have a suppressed cortisol-producing ability. In addition, accumulated lipofuscin formation, telomere shortening and cellular atrophy in zona reticularis cells during aging may account for the age-dependent decline in adrenal androgen levels. In adrenocortical disorders, including both aldosterone-producing adenoma (APA) and cortisol-producing adenoma (CPA), different cellular subtypes of tumor cells presented divergent senescent phenotypes, whereby compact cells in both APA and CPA harbored more senescent phenotypes than clear cells. Autonomous cortisol production from CPA reinforced a local cellular senescence that was more severe than that in APA. Adrenocortical carcinoma (ACC) was also reported to harbor oncogene-induced senescence, which compensatorily follows carcinogenesis and tumor progress. Adrenocortical steroids can induce not only a local senescence but also a periphery senescence in many other tissues. Therefore, herein, we systemically review the recent advances related to cellular senescence in adrenocortical biology and its associated disorders.
Co-Expression Network Analysis of Micro-RNAs and Proteins in the Alzheimer’s Brain: A Systematic Review of Studies in the Last 10 Years
Authors: Tasker Rachel , Rowlands Joseph , Ahmed Zubair , Di Pietro Valentina
Published: December 2021

MicroRNAs (miRNAs) are small non-coding nucleic acids that can regulate post-transcriptional gene expression by binding to complementary sequences of target mRNA. Evidence showed that dysregulated miRNA expression may be associated with neurological conditions such as Alzheimer’s disease (AD). In this study, we combined the results of two independent systematic reviews aiming to unveil the co-expression network of miRNAs and proteins in brain tissues of AD patients. Twenty-eight studies including a total of 113 differentially expressed miRNAs (53 of them validated by qRT-PCR), and 26 studies including a total of 196 proteins differentially expressed in AD brains compared to healthy age matched controls were selected. Pathways analyses were performed on the results of the two reviews and 39 common pathways were identified. A further bioinformatic analysis was performed to match miRNA and protein targets with an inverse relation. This revealed 249 inverse relationships in 28 common pathways, representing new potential targets for therapeutic intervention. A meta-analysis, whenever possible, revealed miR-132-3p and miR-16 as consistently downregulated in late-stage AD across the literature. While no inverse relationships between miR-132-3p and proteins were found, miR-16′s inverse relationship with CLOCK proteins in the circadian rhythm pathway is discussed and therapeutic targets are proposed. The most significant miRNA dysregulated pathway highlighted in this review was the hippo signaling pathway with p = 1.66 × 10−9. Our study has revealed new mechanisms for AD pathogenesis and this is discussed along with opportunities to develop novel miRNA-based drugs to target these pathways.
Monocyte Subsets in Patients with Chronic Heart Failure Treated with Cardiac Resynchronization Therapy
Authors: Ptaszyńska-Kopczyńska Katarzyna , Eljaszewicz Andrzej , Marcinkiewicz-Siemion Marta , Sawicka-Śmiarowska Emilia , Tarasiuk Ewa , Lisowska Anna , Tynecka Marlena , Grubczak Kamil , Radzikowska Urszula , Janucik Adrian , Moniuszko Marcin , Charkiewicz Karol , Laudański Piotr , Sobkowicz Bożena , Kamiński Karol A.
Published: December 2021

Background: The exact role of individual inflammatory factor in heart failure with reduced ejection fraction (HFrEF) remains elusive. The study aimed to evaluate three monocyte subsets (classical-CD14++CD16−, intermediate-CD14++CD16+, and nonclassical-CD14+CD16++) in HFrEF patients and to assess the effect of the cardiac resynchronization therapy (CRT) on the changes in monocyte compartment. Methods: The study included 85 patients with stable HFrEF. Twenty-five of them underwent CRT device implantation with subsequent 6-month assessment. The control group consisted of 23 volunteers without HFrEF. Results: The analysis revealed that frequencies of non-classical-CD14+CD16++ monocytes were lower in HFrEF patients compared to the control group (6.98 IQR: 4.95–8.65 vs. 8.37 IQR: 6.47–9.94; p = 0.021), while CD14++CD16+ and CD14++CD16− did not differ. The analysis effect of CRT on the frequency of analysed monocyte subsets 6 months after CRT device implantation showed a significant increase in CD14+CD16++ (from 7 IQR: 4.5–8.4 to 7.9 IQR: 6.5–9.5; p = 0.042) and CD14++CD16+ (from 5.1 IQR: 3.7–6.5 to 6.8 IQR: 5.4–7.4; p = 0.017) monocytes, while the frequency of steady-state CD14++CD16− monocytes was decreased (from 81.4 IQR: 78–86.2 to 78.2 IQR: 76.1–81.7; p = 0.003). Conclusions: HFrEF patients present altered monocyte composition. CRT-related changes in the monocyte compartment achieve levels observed in controls without HFrEF.
Immunomodulatory Effects Associated with Cladribine Treatment
Authors: Fissolo Nicolás , Calvo-Barreiro Laura , Eixarch Herena , Boschert Ursula , Espejo Carmen , Montalban Xavier , Comabella Manuel
Published: December 2021

Cladribine is a synthetic deoxyadenosine analogue with demonstrated efficacy in patients with relapsing-remitting multiple sclerosis (MS). The main mechanism of action described for cladribine is the induction of a cytotoxic effect on lymphocytes, leading to a long-term depletion of peripheral T and B cells. Besides lymphocyte toxicity, the mode of action may include immunomodulatory mechanisms affecting other cells of the immune system. In order to induce its beneficial effects, cladribine is phosphorylated inside the cell by deoxycytidine kinase (DCK) to its active form. However, the mechanism of action of cladribine may also include immunomodulatory pathways independent of DCK activation. This in vitro study was designed to explore the impact of cladribine on peripheral blood mononuclear cells (PBMC) subsets, and to assess whether the immunomodulatory mechanisms induced by cladribine depend on the activation of the molecule. To this end, we obtained PBMCs from healthy donors and MS patients and performed proliferation, apoptosis and activation assays with clinically relevant concentrations of cladribine in DCK-dependent and -independent conditions. We also evaluated the effect of cladribine on myeloid lineage-derived cells, monocytes and dendritic cells (DCs). Cladribine decreased proliferation and increased apoptosis of lymphocyte subsets after prodrug activation via DCK. In contrast, cladribine induced a decrease in immune cell activation through both DCK-dependent and -independent pathways (not requiring prodrug activation). Regarding monocytes and DCs, cladribine induced cytotoxicity and impaired the activation of classical monocytes, but had no effect on DC maturation. Taken together, these data indicate that cladribine, in addition to its cytotoxic function, can mediate immunomodulation in different immune cell populations, by regulating their proliferation, maturation and activation.
CMV Seropositive Status Increases Heparanase SNPs Regulatory Activity, Risk of Acute GVHD and Yield of CD34+ Cell Mobilization
Authors: Ostrovsky Olga , Beider Katia , Morgulis Yan , Bloom Nira , Cid-Arregui Angel , Shimoni Avichai , Vlodavsky Israel , Nagler Arnon
Published: December 2021

Heparanase is an endo-β-glucuronidase that is best known for its pro-cancerous effects but is also implicated in the pathogenesis of various viruses. Activation of heparanase is a common strategy to increase viral spread and trigger the subsequent inflammatory cascade. Using a Single Nucleotide Polymorphisms (SNP)-associated approach we identified enhancer and insulator regions that regulate HPSE expression. Although a role for heparanase in viral infection has been noticed, the impact of HPSE functional SNPs has not been determined. We investigated the effect of cytomegalovirus (CMV) serostatus on the involvement of HPSE enhancer and insulator functional SNPs in the risk of acute graft versus host disease (GVHD) and granulocyte-colony stimulating factor related CD34+ mobilization. A significant correlation between the C alleles of insulator rs4364254 and rs4426765 and CMV seropositivity was found in healthy donors and patients with hematological malignancies. The risk of developing acute GVHD after hematopoietic stem cell transplantation was identified only in CMV-seropositive patients. A significant correlation between the enhancer rs4693608 and insulator rs28649799 and CD34+ cell mobilization was demonstrated in the CMV-seropositive donors. It is thus conceivable that latent CMV infection modulates heparanase regulatory regions and enhances the effect of functional SNPs on heparanase function in normal and pathological processes.
Pathways in Skeletal Muscle: Protein Signaling and Insulin Sensitivity after Exercise Training and Weight Loss Interventions in Middle-Aged and Older Adults
Authors: Ryan Alice S. , Li Guoyan , McMillin Shawna , Prior Steven J. , Blumenthal Jacob B. , Mastella Laura
Published: December 2021

Aging and obesity contribute to insulin resistance with skeletal muscle being critically important for maintaining whole-body glucose homeostasis. Both exercise and weight loss are lifestyle interventions that can affect glucose metabolism. The purpose of this study was to examine the effects of a six-month trial of aerobic exercise training or weight loss on signaling pathways in skeletal muscle in the basal condition and during hyperinsulinemia during a glucose clamp in middle-aged and older adults. Overweight and obese men and women aged 50–70 years were randomly allocated and completed six months of either weight loss (WL) (n = 18) or 3x/week aerobic exercise training (AEX) (n = 17). WL resulted in 10% weight loss and AEX increased maximal oxygen consumption (VO2max) (both p < 0.001). Insulin sensitivity (hyperinsulinemic-euglycemic 80 mU·m−2·min−1 clamp) increased in WL and AEX (both p < 0.01). In vivo insulin stimulation increased phosphorylation/total protein ratio (P/T) of protein kinase B (Akt), glycogen synthase kinase 3 beta (GSK-β3), 70 kDa ribosomal protein S6 kinase (p70S6k), insulin receptor substrate 1 (IRS-1), and insulin receptor (IR) expression (all p < 0.05) but not P/T extracellular regulated kinase ½ (ERK1/2), c-jun N-terminal kinases (JNK), p38 mitogen-activated protein kinases (p38), or insulin-like growth factor 1 receptor (IGF-1R). There were differences between WL and AEX in the change in basal Akt P/T (p = 0.05), GSK-3β P/T ratio (p < 0.01), p70S6k (p < 0.001), ERK1/2 (p = 0.01) P/T ratio but not p38, JNK, IRS-1, and IGF-1R P/T ratios. There was a difference between WL and AEX in the insulin stimulation changes in GSK3 which increased more after WL than AEX (p < 0.05). In the total group, changes in M were associated with changes in basal total GSK-3β and basal total p70Sk as well as insulin stimulation of total p70Sk. Protein signaling in skeletal muscle provides insight as to mechanisms for improvements in insulin sensitivity in aging and obesity.
Application of the Non-Enzymatic Glucose Sensor Combined with Microfluidic System and Calibration Readout Circuit
Authors: Chou Jung-Chuan , Huang Yu-Hao , Kuo Po-Yu , Lai Chih-Hsien , Nien Yu-Hsun , Chen Yung-Yu , Kang Zhi-Xuan , Lee Kun-Tse
Published: December 2021

In this research, we proposed a potentiometric sensor based on copper doped zinc oxide (CZO) films to detect glucose. Silver nanowires were used to improve the sensor’s average sensitivity, and we used the low power consumption instrumentation amplifier (UGFPCIA) designed by our research group to measure the sensing characteristics of the sensor. It was proved that the sensor performs better when using this system. In order to observe the stability of the sensor, we also studied the influence of two kinds of non-ideal effects on the sensor, such as the drift effect and the hysteresis effect. For this reason, we chose to combine the calibration readout circuit with the voltage-time (V-T) measurement system to optimize the measurement environment and successfully reduced the instability of the sensor. The drift rate was reduced by about 51.1%, and the hysteresis rate was reduced by 13% and 28% at different measurement cycles. In addition, the characteristics of the sensor under dynamic conditions were also investigated, and it was found that the sensor has an average sensitivity of 13.71 mV/mM and the linearity of 0.998 at a flow rate of 5.6 μL/min.
Clinical Significance of the Fetuin-A-to-Adiponectin Ratio in Obese Children and Adolescents with Diabetes Mellitus
Authors: Ahn Moon-Bae , Kim Seul-Ki , Kim Shin-Hee , Cho Won-Kyoung , Suh Jin-Soon , Cho Kyoung-Soon , Suh Byung-Kyu , Jung Min-Ho
Published: December 2021

Fetuin-A and adiponectin are inflammatory cytokines associated with obesity and insulin resistance. This study aimed to examine the fetuin-A-to-adiponectin ratio (FAR) in diabetic children and to determine the role of FAR. A total of 54 children and adolescents with diabetes mellitus (DM) and 44 controls aged 9–16 years were included in this study. Clinical characteristics, including plasma fetuin-A and adiponectin levels, were compared with respect to body mass index (BMI) and diabetes type. Of 98 children, 54.1% were obese, whereas 18.4% were obese and diabetic. FAR was higher in obese children with DM than in non-obese children and also in type 2 DM children than in type 1. FAR showed a stronger association with BMI than with fetuin-A and adiponectin individually, and its association was more prominent in diabetic children than in controls. BMI was a risk factor for increased FAR. Plasma fetuin-A was elevated in obese children, and its association with insulin resistance and β cell function seemed more prominent in diabetic children after adjustment for adiponectin. Thus, FAR could be a useful surrogate for the early detection of childhood metabolic complications in diabetic children, particularly those who are obese.
How Can We Reduce Dental Fear in Children? The Importance of the First Dental Visit
Authors: Carrillo-Díaz María , Migueláñez-Medrán Blanca Carmen , Nieto-Moraleda Carolina , Romero-Maroto Martín , González-Olmo María José
Published: December 2021

Dental fear is a common problem amongst children. It can affect children’s psychological well-being, quality of life, and oral and systemic health. The aim of this study was to identify whether the patients’ age at which visits to the paediatric dentist begin as well as the periodicity of these visits are factors that can prevent dental fear. This observational transversal study was conducted on 575 school children (average age 6.85 ± 0.78) and their mother/father/guardian. Parents completed a survey on the characteristics of dental visits and the child completed the index of dental anxiety and fear (IDAF-4C) to assess dental fear. The correlation between dental fear and age at first visit (r = −0.36 p < 0.01) and dental fear and frequency of visit (r = −0.65 p < 0.01) were statistically significant. The regression analysis performed showed that both variables predicted 44.4% of the dental fear in the child. In conclusion, the age of initiation to the paediatric dentist (before 2 years) and the periodic revisions (every 6 months or every year) could protect the child from dental fear.
Problems, Stressors and Needs of Children and Adolescents with Cancer
Authors: Lewandowska Anna , Zych Barbara , Papp Katalin , Zrubcová Dana , Kadučáková Helena , Šupínová Mária , Apay Serap Ejder , Nagórska Małgorzata
Published: December 2021

Background: Cancer diseases in children and adolescents are considered to be one of the most serious health problems in the world. It is estimated that about 151,435 cases are diagnosed in children annually. Children with cancer experience many comorbid symptoms related to diagnosis and treatment that can profoundly affect their lives. They experience physical and emotional suffering, which affects their well-being and physical fitness, influencing the prognosis and deteriorating their physical, mental and social functioning. Given the limited data, an attempt was made to assess the problems of the biopsychosocial sphere of need and stressors among children and adolescents treated for cancer. Accurate symptom assessment is essential to ensure high-quality care and effective treatment. Patients and Methods: The qualitative study was conducted in pediatric oncology of hospitals in Poland. Children diagnosed with cancer were invited to participate in the study to assess their problems, stressors and needs. Results: The study included 520 people, where female sex constituted 48% and male 52%. The mean age of the children is 13.2 SD = 2.5. Negative experiences related to the disease are experienced by 82% of children. Among the surveyed children, the most experienced were anxiety (61%). The conducted research shows that as many as 69% of all respondents experienced states that indicate severe depression. The most common somatic problems reported by children were pain (58%). The most dominant areas of life that had a negative impact was body image (85%). Conclusions: Children and adolescents diagnosed with neoplastic disease experience many problems and stressors in every sphere of life, which undoubtedly affects a high level of unmet needs. The main category of needs concerning the challenges faced by children with cancer was psychological and care problems. In the youth group, the needs were mainly related to education and social support.
The Effects of Graphene on the Biocompatibility of a 3D-Printed Porous Titanium Alloy
Authors: Sun Xu , Tong Shuang , Yang Shude , Guo Shu
Published: December 2021

3D-printed titanium (Ti) materials have attracted much attention in the field of bone tissue repair. However, the combination strength of traditional alloy materials with bone tissue is lower, and the elastic modulus is higher than that of natural bone tissue, which makes the titanium alloy susceptible to stress shielding phenomena after implantation. Therefore, it is urgent to find better surface modification technology. In this study, the physical and chemical properties, toxicity, and proliferation of adipose stem cells of composite graphene-coated titanium alloy (Gr–Ti) were investigated using 3D-printed titanium alloy as a material model. Physical and chemical property tests confirmed that 3D printing could produce porous titanium alloy materials; the compressive strength and elastic modulus of the titanium alloy scaffolds were 91 ± 3 MPa and 3.1 ± 0.4 GPa, matching the elastic modulus of normal bone tissue. The surface characterization shows that graphene can be coated on titanium alloy by a micro-arc oxidation process, which significantly improves the surface roughness of titanium alloy. The roughness factor (Ra) of the Ti stent was 4.95 ± 1.12 μm, while the Ra of the Gr–Ti stent was 6.37 ± 0.72 μm. After the adipose stem cells were co-cultured with the scaffold for 4 h and 24 h, it was found that the Gr–Ti scaffold could better promote the early cell adhesion. CCK-8 tests showed that the number of ADSCs on the G–Ti scaffold was significantly higher than that on the Ti scaffold (p < 0.01). The relative growth rate (RGR) of ADSCs in Gr–Ti was grade 0–1 (non-toxic). In the in vivo experiment of repairing a critical bone defect of a rabbit mandible, the bone volume fraction in the Gr–Ti group increased to 49.42 ± 3.28%, which was much higher than that in the Ti group (39.76 ± 3.62%) (p < 0.05). In conclusion, the porous graphene–titanium alloy promotes the proliferation and adhesion of adipose stem cells with multidirectional differentiation potential, which has great potential for the application of bone tissue engineering in repairing bone defects in the future.
A Study on the Laser Removal of Epoxy Coatings on SS400 Surface by Beam Scanning Patterns
Authors: Kim Ji-Eon , Lee Jong-Myoung , Hyun Jeong-Hun , Jeong Je-Han , Kim Jong-Do
Published: December 2021

Shipyards are very interested in improving their working environment and resolving environmental pollution issues by replacing mechanical cleaning technologies used before and after painting processes with laser cleaning technology. Because epoxy paint is thickly coated, with a thickness of 200 μm or greater, it is difficult to remove using both laser cleaning and mechanical cleaning technologies. Therefore, this study tried to obtain effective cleaning results by controlling the process parameters when removing the thick epoxy coating using a Q-switching fiber laser cleaning system with an average power of 100 W developed by our research team. The pulse duration time of the laser is 150 ns. Additionally, in order to determine whether the cleaning was sufficient, the difference in laser-induced plume/plasma was compared. By controlling the beam scanning patterns, line overlap rate, and pulse overlap rate, it was possible to obtain effective cleaning results without introducing removal deviation. In addition, the NOP increased when the laser beam overlap rate increased. This increased the amount of heat input to the material and reduced the number of scans required to remove the epoxy paint. As a result of the plume/plasma analysis, less plume/plasma was generated as the paint was removed if the epoxy paint remained on the surface. On the other hand, when all of the paint was removed, a higher brightness of plume/plasma generated by evaporation of the bare metal was observed.
High Performance Accelerated Tests to Evaluate Hard Cr Replacements for Hydraulic Cylinders
Authors: Georgiou Emmanuel P. , Drees Dirk , Timmermans Greet , Zoikis-Karathanasis Alexandros , Pérez-Fernández Marta , Magagnin Luca , Celis Jean-Pierre
Published: December 2021

To prolong the lifetime of hydraulic cylinders, a wear-resistant low-friction surface is required. Until now, hard Cr coatings were the best materials for this. However, in recent years, there has been an increasing pressure on the manufacturing of hard Cr plating and plated products, because of environmental and health hazards. The replacement of these coatings by alternatives has not been highly successful yet, because it requires extensive component testing, which is costly and time-consuming and thus not appropriate for material development. For this reason, there is a high need to develop tribological methods that simulate hydraulic cylinders’ component-testing closely. In addition, these new methods should also provide additional information (e.g., friction evolution) that can assist in the further development and optimization of alternative coatings. Having the above in mind and building on an existing method from the American Society for Testing and Materials (ASTM G133), a new test method that allows users to test directly on hydraulic cylinders was developed. This method can provide a relative ranking of both the wear resistance and frictional performance of alternative coatings in direct comparison to state-of-the-art hard Cr. Importantly, the method is repeatable and has a much shorter test duration than full-scale component tests, thereby accelerating material development significantly.
Trends in Chemical Wood Surface Improvements and Modifications: A Review of the Last Five Years
Authors: Blanchet Pierre , Pepin Simon
Published: December 2021

Increasing the use of wood in buildings is regarded by many as a key solution to tackle climate change. For this reason, a lot of research is carried out to develop new and innovative wood surface improvements and make wood more appealing through features such as increased durability, fire-retardancy, superhydrophobicity, and self-healing. However, in order to have a positive impact on the society, these surface improvements must be applied in real buildings. In this review, the last five years of research in the domain of wood surface improvements and modifications is first presented by sorting the latest innovations into different trends. Afterward, these trends are correlated to specifications representing different normative, ecologic and economic factors which must be considered when expecting to introduce a wood treatment to the market. With this review, the authors hope to help researchers to take into consideration the different factors influencing whether new innovations can leave the research laboratory or not, and thereby facilitate the introduction of new wood surface treatments in the society.
Cosmeceutical Potentials of Grammatophyllum speciosum Extracts: Anti-Inflammations and Anti-Collagenase Activities with Phytochemical Profile Analysis Using an Untargeted Metabolomics Approach
Authors: Yingchutrakul Yodying , Sittisaree Wattanapong , Mahatnirunkul Thanisorn , Chomtong Thitikorn , Tulyananda Tatpong , Krobthong Sucheewin
Published: December 2021

Grammatophyllum speciosum is the largest orchid species and a well-known traditional medicinal plant. Due to skin aging, natural products that inhibit this process can attract the attention of consumers and scientists because radical-scavenging activity, collagenase inhibition, and inflammatory suppression are valuable in dermatological applications. This study investigated the phytochemicals in G. speciosum leaves extracts that have cosmeceutical potentials, including radical-scavenging, anticollagenase, and anti-inflammatory abilities. G. speciosum leaves were extracted using water-based extraction methods. High-resolution mass spectrometry was used to identify the phytochemicals in the extracts. Fibroblast and keratinocyte cell cytotoxicity was determined. Antioxidant abilities were measured using DPPH and ABTS assays. The effect of the extracts on nitric oxide (NO) in macrophage cells was investigated. ELISA of the collagenase enzyme was determined. A total of 721 annotated metabolites were identified in the extracts. Vitexin and orientin were the most abundant metabolites. Cell viability was >80% in both cell lines when the extract concentration was <1 mg/mL. The IC50 values for DPPH and ABTS were 56 and 117 μg/mL, respectively. Furthermore, the extracts revealed that NO and collagenase activity were suppressed by 42% and 23%, respectively. The extracts can suppress ROS, inflammatory, and collagenase activities without causing fibroblast and keratinocyte cell death. Thus, this study provides information on metabolites in G. speciosum leaves, which is promising as cosmeceuticals or pharmaceuticals with anti-inflammatory and anti-collagenase activities.
Crystal Structure, Microstructure and Electronic Properties of a Newly Discovered Ternary Phase in the Al-Cr-Sc System
Authors: Kušter Monika , Meden Anton , Markoli Boštjan , Samardžija Zoran , Vončina Maja , Boulet Pascal , Gaudry Émilie , Dubois Jean-Marie , Šturm Sašo
Published: December 2021

This study focused on the crystal and electronic structures of a newly discovered phase in the Al-Cr-Sc system. The latter two species do not mix in a binary alloy, but can be alloyed with aluminium in the vicinity of the Al2−xCrxSc composition, where 0.3 < x < 0.5. After preparation of the pure constituents via arc melting, high-temperature annealing at 990 °C for 240 h was required to achieve full mixing of the elements. A detailed characterisation of the crystal structure, alloy microstructure and stability was obtained using single-crystal X-ray diffraction (SCXRD) and powder X-ray diffraction (PXRD), in addition to transmission electron microscopy (TEM), especially in high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM) mode, scanning electron microscopy (SEM) with energy-dispersive X-ray spectroscopy (EDXS) and differential scanning calorimetry (DSC) measurements. The crystal structure was refined to a hexagonal unit cell of the MgZn2 type, space group no. 194, P63/mmc, which belongs to the Laves phases family. Special attention was paid to the occupancy of the crystallographic sites that were filled by both Cr and Al atoms. First-principles calculations based on the density functional theory (DFT) were performed to investigate the electronic structure of this ternary phase. The total density of states (DOS) exhibited a pronounced sp character, where a shallow pseudo-gap was visible 0.5 eV below the Fermi energy that brought a small but definite contribution to the thermodynamic stability of the compound.
The Fracture Behavior of 316L Stainless Steel with Defects Fabricated by SLM Additive Manufacturing
Authors: Li Hui , Zhang Jianhao
Published: December 2021

In this paper, the fracture behaviors of 316L stainless steel with defects fabricated by the Selective Laser Melting (SLM) additive manufacturing are studied by a peridynamic method. Firstly, the incremental formulations in the peridynamic framework are presented for the elastic-plastic problems. Then, the pairwise force of a bond for orthotropic material model is proposed according to both the local and the global coordinate systems. A simple three-step approach is developed to describe the void defects that generated in the processing of the SLM additive manufacturing in the numerical model. Next, some representative numerical examples are carried out, whose results explain the validation and accuracy of the present method, and demonstrate that the orthotropic features, micro-cracks and voids of the materials have a significant influence on the ultimate bearing capacity, crack propagation and branching of the corresponding structures. It is also revealed that the crack initiations are induced actively by the defects and the crack branching is contributed to the complex multiple-crack propagation. Finally, the achievements of this paper lay a foundation for the engineering applications of the SLM additive manufacturing materials.
Four Isostructural 3d-4f Mixed Metal Organic Frameworks and Their Magnetic Properties
Authors: Mortensen Marie L. , Lewis Abigail L. , McCandless Gregory , Balkus Kenneth J.
Published: December 2021

Four new isostructural 3d-4f mixed metal organic frameworks (MOFs) Cu Gd2 (BDC)4, compound 1, Cu Ho2 (BDC)4, compound 2, Cu Eu2 (BDC)4, compound 3, and Cu Dy2 (BDC)4, compound 4 were successfully synthesized. The structure, stability, and magnetic properties were analyzed. Each MOF has two lanthanide ions and one copper ion node with terephthalic acid as the organic linker. The lanthanide ions form a dimer with each having a capped trigonal prismatic geometry while the copper ion has a square planar geometry. Each of these MOFs shows varying degrees of antiferromagnetic interactions.
Influence of the Characteristics of Multilayer Interference Antireflection Coatings Based on Nb, Si, and Al Oxides on the Laser-Induced Damage Threshold of ZnGeP2 Single Crystal
Authors: Yudin Nikolai Nikolayevich , Zinoviev Mikhail , Gladkiy Vladislav , Moskvichev Evgeny , Kinyaevsky Igor , Podzyvalov Sergey , Slyunko Elena , Zhuravleva Elena , Pfaf Anastasia , Yudin Nikolai Aleksandrovich , Kulesh Maxim
Published: December 2021

In this work, the effect of the defect structure and the parameters of antireflection interference coatings based on alternating layers of Nb2O5/Al2O3 and Nb2O5/SiO2 layers on the laser-induced damage threshold of ZGP crystals under the action of Ho:YAG laser radiation at a wavelength of 2.097 μm was determined. Coating deposition was carried out using the ion-beam sputtering method. The laser-induced damage threshold of the sample with a coating based on alternating layers Nb2O5 and SiO2 was W0d = 1.8 J/cm2. The laser-induced damage threshold of the coated sample based on alternating layers of Nb2O5 and Al2O3 was W0d = 2.35 J/cm2. It has been found that the presence of silicon conglomerates in an interference antireflection coating leads to a decrease in the laser-induced damage threshold of a nonlinear crystal due to local mechanical stresses and the scattering of incident laser radiation.
Panel Dataset to Assess Proactive Eco-Innovation in the Paradigm of Firm Financial Progression
Authors: Toha Md Abu , Johl Satirenjit Kaur
Published: December 2021

Recently, eco-innovation has received a lot of attention in the academic and corporate world due to its potential to accelerate firm financial progression. To measure eco-innovation, mostly primary data and a reactive approach were employed. By emphasising the proactive approach and utilising a secondary panel dataset, this study fills the existing research gap. Data presented in this paper comprise 31 energy firms from Bursa Malaysia for the years between 2015 and 2019. Panel data associated with eco-innovation proactiveness and firm financial progression were collected from three different sources such as company websites, annual reports, and sustainability reports using content analysis. For data collection, an index was adapted comprising five dimensions of eco-innovation, named as product, process, technology, organizational, and marketing. In addition to that, Tobin’s Q was considered as a proxy dimension for firm financial progression because it considers both market value as well as book value. Following a unit root test, six specific data diagnostic tests were performed to ensure data reliability and validity for future potential usage. The results reveal that the panel dataset was organised and is eligible for further statistical model analysis.
MRI as a Tool to Assess Interstitial Cystitis Associated Bladder and Brain Pathologies
Authors: Towner Rheal A. , Smith Nataliya , Saunders Debra , Hurst Robert E.
Published: December 2021

Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic, often incapacitating condition characterized by pain seeming to originate in the bladder in conjunction with lower urinary tract symptoms of frequency and urgency, and consists of a wide range of clinical phenotypes with diverse etiologies. There are currently no diagnostic tests for IC/BPS. Magnetic resonance imaging (MRI) is a relatively new tool to assess IC/BPS. There are several methodologies that can be applied to assess either bladder wall or brain-associated alterations in tissue morphology and/or pain. IC/BPS is commonly associated with bladder wall hyperpermeability (BWH), particularly in severe cases. Our group developed a contrast-enhanced magnetic resonance imaging (CE-MRI) approach to assess BWH in preclinical models for IC/BPS, as well as for a pilot study for IC/BPS patients. We have also used the CE-MRI approach to assess possible therapies to alleviate the BWH in preclinical models for IC/BPS, which will hopefully pave the way for future clinical trials. In addition, we have used molecular-targeted MRI (mt-MRI) to quantitatively assess BWH biomarkers. Biomarkers, such as claudin-2, may be important to assess and determine the severity of BWH, as well as to assess therapeutic efficacy. Others have also used other MRI approaches to assess the bladder wall structural alterations with diffusion-weighted imaging (DWI), by measuring changes in the apparent diffusion coefficient (ADC), diffusion tensor imaging (DTI), as well as using functional MRI (fMRI) to assess pain and morphological MRI or DWI to assess anatomical or structural changes in the brains of patients with IC/BPS. It would be beneficial if MRI-based diagnostic tests could be routinely used for these patients and possibly used to assess potential therapeutics.
A Scoping Review of Supply Chain Management Systems for Point of Care Diagnostic Services: Optimising COVID-19 Testing Capacity in Resource-Limited Settings
Authors: Maluleke Kuhlula , Musekiwa Alfred , Kgarosi Kabelo , Gregor Emily Mac , Dlangalala Thobeka , Nkambule Sphamandla , Mashamba-Thompson Tivani
Published: December 2021

Background: Point of care (POC) testing has enabled rapid coronavirus disease 2019 (COVID-19) diagnosis in resource-limited settings with limited laboratory infrastructure and high disease burden. However, the accessibility of the tests is not optimal in these settings. This scoping review mapped evidence on supply chain management (SCM) systems for POC diagnostic services to reveal evidence that can help guide future research and inform the improved implementation of SARS-CoV-2 POC diagnostics in resource-limited settings. Methodology: This scoping review was guided by an adapted version of the Arksey and O’Malley methodological framework. We searched the following electronic databases: Medline Ovid, Medline EBSCO, Scopus, PubMed, PsychInfo, Web of Science and EBSCOHost. We also searched grey literature in the form of dissertations/theses, conference proceedings, websites of international organisations such as the World Health Organisation and government reports. A search summary table was used to test the efficacy of the search strategy. The quality of the included studies was appraised using the mixed method appraisal tool (MMAT) version 2018. Results: We retrieved 1206 articles (databases n = 1192, grey literature n = 14). Of these, 31 articles were included following abstract and full-text screening. Fifteen were primary studies conducted in LMICs, and 16 were reviews. The following themes emerged from the included articles: availability and accessibility of POC diagnostic services; reasons for stockouts of POC diagnostic tests (procurement, storage, distribution, inventory management and quality assurance) and human resources capacity in POC diagnostic services. Of the 31 eligible articles, 15 underwent methodological quality appraisal with scores between 90% and 100%. Conclusions: Our findings revealed limited published research on SCM systems for POC diagnostic services globally. We recommend primary studies aimed at investigating the barriers and enablers of SCM systems for POC diagnostic services for highly infectious pathogens such SARS-CoV-2 in high disease-burdened settings with limited laboratory infrastructures.
Quantification of Sodium Relaxation Times and Concentrations as Surrogates of Proteoglycan Content of Patellar CARTILAGE at 3T MRI
Authors: Kamp Benedikt , Frenken Miriam , Henke Jan M. , Abrar Daniel B. , Nagel Armin M. , Gast Lena V. , Oeltzschner Georg , Wilms Lena M. , Nebelung Sven , Antoch Gerald , Wittsack Hans-Jörg , Müller-Lutz Anja
Published: December 2021

Sodium MRI has the potential to depict cartilage health accurately, but synovial fluid can influence the estimation of sodium parameters of cartilage. Therefore, this study aimed to reduce the impact of synovial fluid to render the quantitative compositional analyses of cartilage tissue technically more robust. Two dedicated protocols were applied for determining sodium T1 and T2* relaxation times. For each protocol, data were acquired from 10 healthy volunteers and one patient with patellar cartilage damage. Data recorded with multiple repetition times for T1 measurement and multi-echo data acquired with an additional inversion recovery pulse for T2* measurement were analysed using biexponential models to differentiate longitudinal relaxation components of cartilage (T1,car) and synovial fluid (T1,syn), and short (T2s*) from long (T2l*) transversal relaxation components. Sodium relaxation times and concentration estimates in patellar cartilage were successfully determined: T1,car = 14.5 ± 0.7 ms; T1,syn = 37.9 ± 2.9 ms; c(T1-protocol) = 200 ± 48 mmol/L; T2s* = 0.4 ± 0.1 ms; T2l* = 12.6 ± 0.7 ms; c(T2*-protocol) = 215 ± 44 mmol/L for healthy volunteers. In conclusion, a robust determination of sodium relaxation times is possible at a clinical field strength of 3T to quantify sodium concentrations, which might be a valuable tool to determine cartilage health.
Early Identification and Diagnostic Approach in Acute Respiratory Distress Syndrome (ARDS)
Authors: Arrivé François , Coudroy Rémi , Thille Arnaud W.
Published: December 2021

Acute respiratory distress syndrome (ARDS) is a life-threatening condition defined by the acute onset of severe hypoxemia with bilateral pulmonary infiltrates, in the absence of a predominant cardiac involvement. Whereas the current Berlin definition was proposed in 2012 and mainly focused on intubated patients under invasive mechanical ventilation, the recent COVID-19 pandemic has highlighted the need for a more comprehensive definition of ARDS including patients treated with noninvasive oxygenation strategies, especially high-flow nasal oxygen therapy, and fulfilling all other diagnostic criteria. Early identification of ARDS in patients breathing spontaneously may allow assessment of earlier initiation of pharmacological and non-pharmacological treatments. In the same way, accurate identification of the ARDS etiology is obviously of paramount importance for early initiation of adequate treatment. The precise underlying etiological diagnostic (bacterial, viral, fungal, immune, malignant, drug-induced, etc.) as well as the diagnostic approach have been understudied in the literature. To date, no clinical practice guidelines have recommended structured diagnostic work-up in ARDS patients. In addition to lung-protective ventilation with the aim of preventing worsening lung injury, specific treatment of the underlying cause has a central role to improve outcomes. In this review, we discuss early identification of ARDS in non-intubated patients breathing spontaneously and propose a structured diagnosis work-up.