bluuebunny/crossref_metadata_2025 huggingface dataset:
FACTORS THAT INFLUENCE THE DECISION TO ELECT YOUNG VOTERS IN THE 2020 ELECTIONS
Authors: Citrayanti Sherly Alifah , Yuhertiana Indrawati
Published: January 2022

The Covid-19 pandemic has made the government work harder to adapt to conditions that are still critical. All areas of life have undergone very significant changes so that the government pays special attention to immediately make new policies by innovating so that everything returns to normal. In times like today, the government needs an update that brings changes for the better. Brilliant thoughts and ideas are needed to be able to lift Indonesia to adapt to the situation. One of them can start by improving human resources in the government sector with the implementation of the simultaneous Regional Head Elections in 2020 later, the elected people will become the channel for public aspirations to work together in synergy to overcome the current pandemic situation. Public participation is needed to choose which candidates are ready to devote their bodies and mind to Indonesia. Therefore, it is important to know the track record of regional head candidates because it will impact their performance in the next leadership period. In addition, social political culture can shape the personality and thoughts of a group. The purpose of this study is to find out and empirically test the influence of the track record of regional head candidates, government financial information, and political culture on the decision to choose young voters in the 2020 Pilkada. The method used in this research is quantitative with a questionnaire technique and analyzed by Smart PLS. The population used active students class 2017 at UPN "Veteran" East Java. The results show that work experience, education level, socio-economic background of regional head candidates, government financial information, and political culture positively and significantly impact the decision to choose young voters in the 2020 simultaneous elections.

Keywords: Track Record, Financial Information, Political Culture, Regional Head Election 2020, Luder Contingency Model
REBRANDING BADAN KEPENDUDUKAN DAN KELUARGA BERENCANA NASIONAL DALAM MENYASAR GENERASI MILLENIAL DAN ZILLENIAL
Authors: Fauzia Rizky
Published: January 2022

Penelitian ini bertujuan untuk mendeskripsikan proses rebranding Badan Kependudukan dan Keluarga Berencana Nasional (BKKBN) dengan menggunakan metode penelitian deskriptif kualitatif. Teknik pengumpukan data dilakukan dengan cara studi dokumen dan observasi dengan terjun langsung di lapangan. Hasil penelitian menunjukkan bahwa rebranding BKKBN sudah dimulai pada tahun 2009 pascareformasi atau setelah diterapkannya Otonomi Daerah di Indonesia. Namun pada tahun 2019 BKKBN menginisiasikan formalitas rebranding dalam peraturan lembaga. Penelitian ini menjelaskan secara detail pelaksanaan rebranding BKKBN dengan melakukan empat elemen rebranding yaitu repositioning, renaming, redesign, dan relaunch. Hasil penelitian menunjukkan bahwa repositioning BKKBN dilakukan dengan menentukan target segmentasi audiens BKKBN yang berfokus pada generasi millenial dan zillenial serta mengubah posisi BKKBN dari isu fertilitas menjadi “Sahabat Keluarga”. Pada proses renaming BKKBN tidak mengubah namanya karena UU No. 52 Tahun 2009 masih relevan sehingga yang diganti hanyalah visi dan misi BKKBN agar lebih relatable dengan kondisi saat ini. Pada tahap redesign BKKBN melibatkan publik dengan mengadakan lomba logo, tagline, dan jingle, expert meeting serta pre-testing hasil lomba. Logo baru BKKBN berbentuk hati, kupu-kupu dan berwarna biru, tagline BKKBN adalah “Berencana itu Keren”. Proses relaunch menemui banyak hambatan dari pengunduran karena pematangan konsep logo, tagline, dan theme song serta pandemi Covid-19. Soft launching pemenang jingle lomba dan aransemen ulang Mars KB diadakan pada 19 Desember 2019. Peluncuran logo baru BKKBN dilakukan di internal BKKBN, seluruh media sosial @BKKBNOfficial dan penyebarluasan siaran pers. Terakhir Grand launching rebranding diadakan berbarengan dengan Hari Keluarga Nasional ke-XXVI pada 29 Juni 2020.

Kata kunci: rebranding, komunikasi, BKKBN, brand image
A positive Selection Escherichia Coli Recombinant Protein Expression Vector for One-Step Cloning
Authors: James Shinto , Jain Vikas
Published: January 2022

We introduce OLIVAR (Orientation seLection of Insert in Vector through Antisense Reporter) as a novel selection strategy for the insertion of protein-coding genes into vector backbones. As a proof-of-concept, we have engineered a plasmid vector, pGRASS (Green fluorescent protein Reporter from Antisense promoter-based Screening System), for gene cloning in E. coli. With pGRASS, positive clones can be effortlessly distinguished from negative clones after blunt-end cloning. The vector not only screens clones with an insert but also for its correct orientation. The design further allows for the expression of recombinant protein from the T7 promoter in an appropriate host bacterium. With this vector, we are able to reduce the entire cloning workflow into a single step involving a 2-h reaction at room temperature. We believe that our cloning-cum-screening system presented here is extremely cost-effective and straightforward and can be applied to other vector systems and domains such as phage display and library construction.
Single-Atom Fe-Anchored Nano-Diamond With Enhanced Dual-Enzyme Mimicking Performance for H2O2 and Glutathione Detection
Authors: Liu Ying , Yan Jianghong , Huang Yu , Sun Zhiheng , Zhang Huijing , Fu Lihaoyuan , Li Xuwen , Jin Yongri
Published: January 2022

Glutathione (GSH) is an important antioxidant and free radical scavenger that converts harmful toxins into harmless substances and excretes them out of the body. In the present study, we successfully prepared single-atom iron oxide-nanoparticle (Fe-NP)-modified nanodiamonds (NDs) named Fe-NDs via a one-pot in situ reduction method. This nanozyme functionally mimics two major enzymes, namely, peroxidase and oxidase. Accordingly, a colorimetric sensing platform was designed to detect hydrogen peroxide (H2O2) and GSH. Owing to their peroxidase-like activity, Fe-NDs can oxidize colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into blue with sufficient linearity at H2O2 concentrations of 1–60 μM and with a detection limit of 0.3 μM. Furthermore, using different concentrations of GSH, oxidized TMB can be reduced to TMB, and the color change from blue to nearly colorless can be observed by the naked eye (linear range, 1–25 μM; detection limit, 0.072 μM). The established colorimetric method based on oxidase-like activity can be successfully used to detect reduced GSH in tablets and injections with good selectivity and high sensitivity. The results of this study exhibited reliable consistency with the detection results obtained using high-performance liquid chromatography (HPLC). Therefore, the Fe-NDs colorimetric sensor designed in this study offers adequate accuracy and sensitivity.
Evaluation of Analytical Performances of Magnetic Force-Assisted Electrochemical Sandwich Immunoassay for the Quantification of Carcinoembryonic Antigen
Authors: Hwang Boo Young , Kim Eunsoo , Kim Seung-ha , Hwang Hyundoo
Published: January 2022

Carcinoembryonic antigen (CEA) is a biomarker indicated in different cancers, targeted for quantitative analysis via immunoassay. Here we introduce a new technique called magnetic force-assisted electrochemical sandwich immunoassay (MESIA) for determination of CEA level in a drop of human serum using a fully automated point-of-care testing (POCT) device. The analytical performances of the assay are assessed based on precision, accuracy, limit of blank (LoB), limit of detection (LoD) and limit of quantitation (LoQ), linearity, Hook effect, interference, cross-reactivity, and method comparison following the guidelines of the Clinical Laboratory Standards Institute (CLSI). The LoD is 0.50 ng/ml. A linear relationship is shown in the range of 0.5–200 ng/ml. A high dose effect is not seen up to approximately 500,000 ng/ml. The recovery range is from 94.7 to 108.9%. The %CV of run-to-run and within-lab variations are less than 2.04 and 4.41% across the CEA concentrations, respectively, whereas reproducibility is 4.45–6.24%. Method comparison shows that the assay correlates well with the reference device (R2 = 0.9884). The assay demonstrates acceptable precision, accuracy, LoB, LoD and LoQ, hook effect, linearity, interference, cross-reactivity, and high correlation with its reference device. Thus, the system is suitable for the quantification of CEA in clinical practices with a POCT manner.
Community Currencies as Crisis Response: Results From a Randomized Control Trial in Kenya
Authors: Mqamelo Rebecca
Published: January 2022

This paper presents the results of what may be the world’s first randomized control trial on community currencies, focusing on Grassroots Economics Community Inclusion Currency (CIC) model run on the xDAI blockchain. Beneficiaries in Nairobi, Kenya were sent the equivalent of $30 in cryptocurrency tokens, enabling a level of impact evaluation usually unfeasible for most cash and mobile-money based transfer programs. Results show that CIC transfers of $30 are associated with $93.51 increase in beneficiaries wallet balance, a $23.17 increase in monthly CIC income, a $16.30 increase in monthly CIC spending, a $6.31 increase in average trade size and a $28.43 increase in expenditure on food and water. However, the difference in treatment effects for males versus females suggests gender imbalances persist. This study serves as an important prototype for novel cash transfer models and presents some of the first quantitative evidence in the area of “crypto for good.”
LanB1 Cooperates With Kon-Tiki During Embryonic Muscle Migration in Drosophila
Authors: Pérez-Moreno Juan José , Santa-Cruz Mateos Carmen , Martín-Bermudo María Dolores , Estrada Beatriz
Published: January 2022

Muscle development is a multistep process that involves cell specification, myoblast fusion, myotube migration, and attachment to the tendons. In spite of great efforts trying to understand the basis of these events, little is known about the molecular mechanisms underlying myotube migration. Knowledge of the few molecular cues that guide this migration comes mainly from studies in Drosophila. The migratory process of Drosophila embryonic muscles involves a first phase of migration, where muscle progenitors migrate relative to each other, and a second phase, where myotubes migrate searching for their future attachment sites. During this phase, myotubes form extensive filopodia at their ends oriented preferentially toward their attachment sites. This myotube migration and the subsequent muscle attachment establishment are regulated by cell adhesion receptors, such as the conserved proteoglycan Kon-tiki/Perdido. Laminins have been shown to regulate the migratory behavior of many cell populations, but their role in myotube migration remains largely unexplored. Here, we show that laminins, previously implicated in muscle attachment, are indeed required for muscle migration to tendon cells. Furthermore, we find that laminins genetically interact with kon-tiki/perdido to control both myotube migration and attachment. All together, our results uncover a new role for the interaction between laminins and Kon-tiki/Perdido during Drosophila myogenesis. The identification of new players and molecular interactions underlying myotube migration broadens our understanding of muscle development and disease.
Perfused Platforms to Mimic Bone Microenvironment at the Macro/Milli/Microscale: Pros and Cons
Authors: Lipreri Maria Veronica , Baldini Nicola , Graziani Gabriela , Avnet Sofia
Published: January 2022

As life expectancy increases, the population experiences progressive ageing. Ageing, in turn, is connected to an increase in bone-related diseases (i.e., osteoporosis and increased risk of fractures). Hence, the search for new approaches to study the occurrence of bone-related diseases and to develop new drugs for their prevention and treatment becomes more pressing. However, to date, a reliable in vitro model that can fully recapitulate the characteristics of bone tissue, either in physiological or altered conditions, is not available. Indeed, current methods for modelling normal and pathological bone are poor predictors of treatment outcomes in humans, as they fail to mimic the in vivo cellular microenvironment and tissue complexity. Bone, in fact, is a dynamic network including differently specialized cells and the extracellular matrix, constantly subjected to external and internal stimuli. To this regard, perfused vascularized models are a novel field of investigation that can offer a new technological approach to overcome the limitations of traditional cell culture methods. It allows the combination of perfusion, mechanical and biochemical stimuli, biological cues, biomaterials (mimicking the extracellular matrix of bone), and multiple cell types. This review will discuss macro, milli, and microscale perfused devices designed to model bone structure and microenvironment, focusing on the role of perfusion and encompassing different degrees of complexity. These devices are a very first, though promising, step for the development of 3D in vitro platforms for preclinical screening of novel anabolic or anti-catabolic therapeutic approaches to improve bone health.
The Clock Takes Shape—24 h Dynamics in Genome Topology
Authors: Tartour Kévin , Padmanabhan Kiran
Published: January 2022

Circadian rhythms orchestrate organismal physiology and behavior in order to anticipate daily changes in the environment. Virtually all cells have an internal rhythm that is synchronized every day by Zeitgebers (environmental cues). The synchrony between clocks within the animal enables the fitness and the health of organisms. Conversely, disruption of rhythms is linked to a variety of disorders: aging, cancer, metabolic diseases, and psychological disorders among others. At the cellular level, mammalian circadian rhythms are built on several layers of complexity. The transcriptional-translational feedback loop (TTFL) was the first to be described in the 90s. Thereafter oscillations in epigenetic marks highlighted the role of chromatin state in organizing the TTFL. More recently, studies on the 3D organization of the genome suggest that genome topology could be yet another layer of control on cellular circadian rhythms. The dynamic nature of genome topology over a solar day implies that the 3D mammalian genome has to be considered in the fourth dimension-in time. Whether oscillations in genome topology are a consequence of 24 h gene-expression or a driver of transcriptional cycles remains an open question. All said and done, circadian clock-gated phenomena such as gene expression, DNA damage response, cell metabolism and animal behavior—go hand in hand with 24 h rhythms in genome topology.
Exosomes Regulate NLRP3 Inflammasome in Diseases
Authors: Li Zhangwang , Chen Xinyue , Tao Junjie , Shi Ao , Zhang Jing , Yu Peng
Published: January 2022

Emerging evidence has suggested the unique and critical role of exosomes as signal molecules vector in various diseases. Numerous researchers have been trying to identify how these exosomes function in immune progression, as this could promote their use as biomarkers for the disease process and potential promising diagnostic tools. NOD-like receptor (NLR) family, pyrin domain containing 3 (NLRP3), a tripartite protein, contains three functional domains a central nucleotide-binding and oligomerization domain (NACHT), an N-terminal pyrin domain (PYD), and a leucine-rich repeat domain (LRR). Of note, existing studies have identified exosome as a novel mediator of the NLRP3 inflammasome, which is critical in diseases progression. However, the actual mechanisms and clinical treatment related to exosomes and NLRP3 are still not fully understood. Herein, we presented an up-to-date review of exosomes and NLRP3 in diseases, outlining what is known about the role of exosomes in the activation of NLRP3 inflammasome and also highlighting areas of this topic that warrant further study.
Effect of Trastuzumab–HER2 Complex Formation on Stress-Induced Modifications in the CDRs of Trastuzumab
Authors: Spanov Baubek , Aboagye Victoria , Olaleye Oladapo , Govorukhina Natalia , van de Merbel Nico C. , Bischoff Rainer
Published: January 2022

Asparagine deamidation and aspartic acid isomerization in the complementarity determining regions (CDRs) of monoclonal antibodies may alter their affinity to the target antigen. Trastuzumab has two hot spots for deamidation and one position for isomerization in the CDRs. Little is known how complex formation with its target antigen HER2 affects these modifications. Modifications in the CDRs of trastuzumab were thus compared between the free antibody and the trastuzumab–HER2 complex when stressed under physiological conditions at 37°C. Complex formation and stability of the complex upon stressing were assessed by size-exclusion chromatography. Deamidation of light-chain Asn-30 (Lc-Asn-30) was extensive when trastuzumab was stressed free but reduced about 10-fold when the antibody was stressed in complex with HER2. Almost no deamidation of heavy-chain (Hc-Asn-55) was detected in the trastuzumab–HER2 complex, while deamidation was observed when the antibody was stressed alone. Hc-Asp-102 isomerization, a modification that critically affects biological activity, was observed to a moderate degree when the free antibody was stressed but was not detected at all in the trastuzumab–HER2 complex. This shows that complex formation has a major influence on critical modifications in the CDRs of trastuzumab.
Clearance or Hijack: Universal Interplay Mechanisms Between Viruses and Host Autophagy From Plants to Animals
Authors: Wu Wenxian , Luo Xiumei , Ren Maozhi
Published: January 2022

Viruses typically hijack the cellular machinery of their hosts for successful infection and replication, while the hosts protect themselves against viral invasion through a variety of defense responses, including autophagy, an evolutionarily ancient catabolic pathway conserved from plants to animals. Double-membrane vesicles called autophagosomes transport trapped viral cargo to lysosomes or vacuoles for degradation. However, during an ongoing evolutionary arms race, viruses have acquired a strong ability to disrupt or even exploit the autophagy machinery of their hosts for successful invasion. In this review, we analyze the universal role of autophagy in antiviral defenses in animals and plants and summarize how viruses evade host immune responses by disrupting and manipulating host autophagy. The review provides novel insights into the role of autophagy in virus–host interactions and offers potential targets for the prevention and control of viral infection in both plants and animals.
LAPped in Proof: LC3‐Associated Phagocytosis and the Arms Race Against Bacterial Pathogens
Authors: Grijmans Bart J. M. , van der Kooij Sander B. , Varela Monica , Meijer Annemarie H.
Published: January 2022

Cells of the innate immune system continuously patrol the extracellular environment for potential microbial threats that are to be neutralized by phagocytosis and delivery to lysosomes. In addition, phagocytes employ autophagy as an innate immune mechanism against pathogens that succeed to escape the phagolysosomal pathway and invade the cytosol. In recent years, LC3-associated phagocytosis (LAP) has emerged as an intermediate between phagocytosis and autophagy. During LAP, phagocytes target extracellular microbes while using parts of the autophagic machinery to label the cargo-containing phagosomes for lysosomal degradation. LAP contributes greatly to host immunity against a multitude of bacterial pathogens. In the pursuit of survival, bacteria have developed elaborate strategies to disarm or circumvent the LAP process. In this review, we will outline the nature of the LAP mechanism and discuss recent insights into its interplay with bacterial pathogens.
Culture and Social Norms: Development and Application of a Model for Culturally Contextualized Communication Measurement (MC3M)
Authors: Liu Rain W. , Lapinski Maria K. , Kerr John M. , Zhao Jinhua , Bum Tsering , Lu Zhi
Published: January 2022

Studies of social norms are common in the communication literature and are increasingly focused on cultural dynamics: studying co-cultural groups within national boundaries or comparing countries. Based on the review of the status quo in cross-cultural measurement development and our years of experience in conducting this research among a co-cultural group, this paper describes a Model for Culturally Contextualized Communication Measurement (MC3M) for intercultural and/or cross-cultural communication research. As an exemplar, we report on a program of research applying the model to develop a culturally derived measurement of social norms and the factors impacting the norm-behavior relationship for members of a unique population group (i.e., ethnically Tibetan pastoralists in Western China). The results provide preliminary evidence for the construct validity and reliability of the culturally derived measurements. The implications, benefits, and shortcomings of the MC3M model are discussed. Recommendations for advancing both conceptual and measurement refinement in intercultural and cross-cultural communication research are provided.
Awer Honey-Hunting Culture With Greater Honeyguides in Coastal Kenya
Authors: van der Wal Jessica E. M. , Gedi Isa I. , Spottiswoode Claire N.
Published: January 2022

The remarkable mutualism between humans and greater honeyguides (Indicator indicator) is known still to thrive in only a few places in Africa. Here, we report on the honey-hunting culture of the marginalised Awer people in Kenya, historically a hunter-gatherer culture who today practise a mixed economy including significant amounts of foraging for wild foods. As part of a larger effort to document cross-cultural honey-hunting traditions in Africa, we interviewed six Awer honey-hunters to document their cultural practices. The interviewees reported that they depend on wild honey as a source of income, and that they readily seek the cooperation of honeyguides. Honey-hunting skills and the calls/whistles used to communicate with honeyguides are learnt from their fathers and other elders in village. The best time to honey-hunt is in the months following the big rains (August–December), when interviewees go out honey-hunting once a week on average. Honeyguides are not actively rewarded with wax, as it is believed that once a bird is fed it will not cooperate again for some time, and therefore after the honey harvest is complete, all remaining wax comb is buried. Honey-hunting practices are declining in this region, which interviewees attributed to drought and a lack of interest by the youth. These findings expand our understanding of how human-honeyguide mutualism persists across a range of human cultural variation.
Alterations of Gut Microbiome and Serum Metabolome in Coronary Artery Disease Patients Complicated With Non-alcoholic Fatty Liver Disease Are Associated With Adverse Cardiovascular Outcomes
Authors: Hu Xiaomin , Zhou Ruilin , Li Hanyu , Zhao Xinyue , Sun Yueshen , Fan Yue , Zhang Shuyang
Published: January 2022

Rationale: Patients suffering from coronary artery disease (CAD) complicated with nonalcoholic fatty liver disease (NAFLD) present worse cardiovascular outcomes than CAD patients without NAFLD. The progression of CAD is recently reported to be associated with gut microbiota and microbe-derived metabolites. However, it remains unclear how the complication of NAFLD will affect gut microbiota and microbe-derived metabolites in CAD patients, and whether or not this interplay is related to the worse cardiovascular outcomes in CAD-NAFLD patients.

Methods: We performed 16S rRNA sequencing and serum metabolomic analysis in 27 CAD patients with NAFLD, 81 CAD patients without NAFLD, and 24 matched healthy volunteers. Predicted functional profiling was achieved using PICRUSt2. The occurrence of cardiovascular events was assessed by a follow-up study. The association of alterations in the gut microbiome and metabolome with adverse cardiovascular events and clinical indicators was revealed by Spearman correlation analysis.

Results: We discovered that the complication of NAFLD was associated with worse clinical outcomes in CAD patients and critical serum metabolome shifts. We identified 25 metabolite modules that were correlated with poor clinical outcome in CAD-NAFLD patients compared with non-NAFLD patients, represented by increased cardiac-toxic metabolites including prochloraz, brofaromine, aristolochic acid, triethanolamine, and reduced potentially beneficial metabolites including estradiol, chitotriose, palmitelaidic acid, and moxisylyte. In addition, the gut microbiome of individuals with CAD-NAFLD was changed and characterized by increased abundances of Oscillibacter ruminantium and Dialister invisus, and decreased abundances of Fusicatenibacter saccharivorans, Bacteroides ovatus and Prevotella copri. PICRUSt2 further confirmed an increase of potential pathogenic bacteria in CAD-NAFLD. Moreover, we found that variations of gut microbiota were critically correlated with changed circulating metabolites and clinical outcomes, which revealed that aberrant gut microbiota in CAD-NAFLD patients may sculpt a detrimental metabolome which results in adverse cardiovascular outcomes.

Conclusions: Our findings suggest that CAD patients complicated with NAFLD result in worse clinical outcomes possibly by modulating the features of the gut microbiota and circulating metabolites. We introduce “liver-gut microbiota-heart axis” as a possible mechanism underlying this interrelationship. Our study provides new insights on the contribution of gut microbiota heterogeneity to CAD-NAFLD progression and suggests novel strategies for disease therapy.
Assessing the Geothermal Resource Potential of an Active Oil Field by Integrating a 3D Geological Model With the Hydro-Thermal Coupled Simulation
Authors: Huang Yonghui , Cheng Yuanzhi , Ren Lu , Tian Fei , Pan Sheng , Wang Ke , Wang Jianwei , Dong Yuexia , Kong Yanlong
Published: January 2022

Assessment of available geothermal resources in the deep oil field is important to the synergy exploitation of oil and geothermal resources. A revised volumetric approach is proposed in this work for evaluating deep geothermal potential in an active oil field by integrating a 3D geological model into a hydrothermal (HT)-coupled numerical model. Based on the analysis of the geological data and geothermal conditions, a 3D geological model is established with respect to the study area, which is discretized into grids or elements represented in the geological model. An HT-coupled numerical model was applied based on the static geological model to approximate the natural-state model of the geothermal reservoir, where the thermal distribution information can be extracted. Then the geothermal resource in each small grid element is calculated using a volumetric method, and the overall geothermal resource of the reservoirs can be obtained by making an integration over each element of the geological model. A further parametric study is carried out to investigate the influence of oil and gas saturations on the overall heat resources. The 3D geological model can provide detailed information on the reservoir volume, while the HT natural-state numerical model addressed the temperature distribution in the reservoir by taking into account complex geological structures and contrast heterogeneity. Therefore, integrating the 3D geological modeling and HT numerical model into the geothermal resource assessment improved its accuracy and helped to identify the distribution map of the available geothermal resources, which indicate optimal locations for further development and utilization of the geothermal resources. The Caofeidian new town Jidong oil field serves as an example to depict the calculation workflow. The simulation results demonstrate in the Caofeidian new town geothermal reservoir that the total amount of geothermal resources, using the proposed calculation method, is found to be 1.23e+18 J, and the total geothermal fluid volume is 8.97e+8 m3. Moreover, this approach clearly identifies the regions with the highest potential for geothermal resources. We believe this approach provides an alternative method for geothermal potential assessment in oil fields, which can be also applied globally.
CO2 Mass Transfer and Oil Replacement Capacity in Fractured Shale Oil Reservoirs: From Laboratory to Field
Authors: Qiao Runwei , Li Fengxia , Zhang Shicheng , Wang Haibo , Wang Fei , Zhou Tong
Published: January 2022

CO2-based fracturing is widely introduced to stimulate shale oil reservoirs for its multiple advantages. However, the range of CO2 entering the matrix around fractures and CO2-oil replacement capacity between matrix and fractures cannot be fully explained. To address this issue, a radial constant volume diffusion experiment on shale cores was designed in this study, and the pressure drop curve history was matched through numerical model to determine the composition effective diffusion coefficient. A field-scale numerical model was established, in which a series of certain grids were used to explicitly characterize fracture and quantify the prosess of CO2 mass transfer and oil replacement. Based on the field-scale numerical model, the process of shut-in, flow back, and oil production was simulated. The distribution of CO2 in fractured shale oil formation and its impact on crude oil during shut-in stage and flow back stage were investigated. This study concludes that CO2 gradually exchanges the oil in matrix into fractures and improve the fluidity of oil in matrix until the component concentrations of the whole reservoir reaches equilibrium during the shut-in process. Finally, about 30∼35 mole % of CO2 in fractures exchanges for oil in matrix. The range of CO2 entering the matrix around fractures is only 1.5 m, and oil in matrix beyond this distance will not be affected by CO2. During the process of flow back and production, the CO2 in fracture flows back quickly, but the CO2 in matrix is keeping dissolved in oil and will not be quickly produced. It is conclued that the longest possible shut-in time is conducive to making full use of the CO2-EOR mechanism in fractured shale oil reservoirs. However, due to the pursuit of economic value, a shut-in time of 10 days is the more suitable choice. This work can provide a better understanding of CO2 mass transfer mechanism in fractured shale oil reservoirs. It also provides a reference for the evaluation of the shut-in time and production management after CO2 fracturing.
Prediction and Treatment of Water Leakage Risk Caused by the Dynamic Evolution of Ground Fissures in Gully Terrain
Authors: Feng Dong , Hou Enke , Xie Xiaoshen , Che Xiaoyang , Hou Pengfei , Long Tianwen
Published: January 2022

Groundwater leakage in the loess gully terrain is one of the main hazards of coal seam mining at shallow burial depth. The burial depth of the 5−2 coal seam is less than 50 m from the ground in the gully of the study site. The fissures that expand upward after mining can easily penetrate the ground to form a water-conducting channels. During rainy periods, there is a potential risk of groundwater leakage. In order to reveal the characteristics of plane development and the dynamic evolution of gully ground fissures, the typical U-shaped gully in the northern Shaanxi coal mine was studied using the field measurement methods of “On-site measurement” and UAV aerial photography. Based on the experimental platform of ground fissure leakage developed and designed by the team, an indoor test model corresponding to the actual situation was established. In addition, the mathematical models of actual flood inrush, fissure width, and flood flow in the channel were established. The actual mine water flow and the mine drainage capacity were compared and analyzed, thus proposing criteria for classifying gully mining ground fissure collapsed water hazards. These criteria can provide theoretical references for predicting fissure leakage hazard zones in the ground gully of shallow buried coal seams. According to the development height of the water-conducting fissure zone (WCFZ), the treatment methods of ground fissures in gullies under different security conditions were designed, which was applied in the field with good results. The results showed that the treatment methods in this paper could effectively prevent the leakage of groundwater in the gullies along the ground fissures.
Causes and Effects of Oocyte Retrieval Difficulties: A Retrospective Study of 10,624 Cycles
Authors: Wang Yang , Zhang Meixiang , Shi Hao , Yi Shiqi , Li Qian , Su Yingchun , Guo Yihong , Hu Linli , Sun Jing , Sun Ying-pu
Published: January 2022

Oocyte retrieval is a routine procedure during the application of assisted reproduction technology. However, technical difficulties experienced during oocyte retrieval and the subsequent unsatisfactory number of oocytes obtained are rarely reported. The current study included 10,624 oocyte retrieval cycles from April 2015 to June 2018, and patients were followed up until February 2019. Patients were divided into two groups depending on whether the oocyte number obtained reached the >14-mm follicle number on the day of hCG administration. In the oocyte retrieval not satisfactory (ORNS) group, there were 1,294 cycles, and in the oocyte retrieval satisfactory (ORS) group, there were 9,330 cycles. ORNS patients were older, had a longer duration of infertility, had higher follicle-stimulating hormone, and were more likely to have endometriosis. The ORS group had a higher rate of the use of a follicular phase long-acting gonadotropin-releasing hormone (GnRH) agonist long ovarian stimulation protocol and a lower rate of the use of a luteal phase short-acting GnRH agonist long protocol. The ORNS group had fewer total number of days of FSH stimulation. On human chorionic gonadotropin day, the ORNS group had higher luteinizing hormone (LH), lower estradiol, and lower progesterone levels. After oocyte retrieval, the oocyte quality and fresh cycle transplantation rate were higher in the ORNS group. An unsatisfactory oocyte retrieval number did not influence the clinical pregnancy rate, miscarriage rate, or live birth rate during the fresh cycles. The cumulative pregnancy rate and the live birth rate were lower in the ORNS group. In conclusion, with a similar number of matured follicles, ORNS was more likely to occur in ovarian dysfunction patients. The follicular phase long-acting GnRH agonist long protocol had lower oocyte retrieval difficulty during IVF/ICSI. ORNS does not affect embryo quality or the fresh cycle pregnancy rate, but it significantly reduces the cumulative pregnancy rate and the live birth rate.
Molecular and Cellular Bases of Lipodystrophy Syndromes
Authors: Zammouri Jamila , Vatier Camille , Capel Emilie , Auclair Martine , Storey-London Caroline , Bismuth Elise , Mosbah Héléna , Donadille Bruno , Janmaat Sonja , Fève Bruno , Jéru Isabelle , Vigouroux Corinne
Published: January 2022

Lipodystrophy syndromes are rare diseases originating from a generalized or partial loss of adipose tissue. Adipose tissue dysfunction results from heterogeneous genetic or acquired causes, but leads to similar metabolic complications with insulin resistance, diabetes, hypertriglyceridemia, nonalcoholic fatty liver disease, dysfunctions of the gonadotropic axis and endocrine defects of adipose tissue with leptin and adiponectin deficiency. Diagnosis, based on clinical and metabolic investigations, and on genetic analyses, is of major importance to adapt medical care and genetic counseling. Molecular and cellular bases of these syndromes involve, among others, altered adipocyte differentiation, structure and/or regulation of the adipocyte lipid droplet, and/or premature cellular senescence. Lipodystrophy syndromes frequently present as systemic diseases with multi-tissue involvement. After an update on the main molecular bases and clinical forms of lipodystrophy, we will focus on topics that have recently emerged in the field. We will discuss the links between lipodystrophy and premature ageing and/or immuno-inflammatory aggressions of adipose tissue, as well as the relationships between lipomatosis and lipodystrophy. Finally, the indications of substitutive therapy with metreleptin, an analog of leptin, which is approved in Europe and USA, will be discussed.
A Symmetry and Asymmetry Investigation of the Nexus Between Environmental Sustainability, Renewable Energy, Energy Innovation, and Trade: Evidence From Environmental Kuznets Curve Hypothesis in Selected MENA Countries
Authors: Andriamahery Anselme , Qamruzzaman Md.
Published: January 2022

The motivation of the study is to gauge the role of renewable energy consumption (REC), energy innovation (EI), and total trade (TR) on environmental sustainability (ES) in selected MENA (Middle East and North Africa) countries for the period 1980–2018 under the assumption of environmental Kuznets curve (EKC). The study implemented several econometrical tools, including structural break unit root test, Bayer–Hanck combined cointegration test, autoregressive distributed lag (ARDL), nonlinear ARDL, and Granger causality test under error correction term. Variables properties test detected that all the variables are stationary after the first difference but neither exposed to stationary after the second difference. The test statistics of the combined cointegration test documented a long-run association between ES, RE, EI, and TR, which is valid for both countries concerned. Regarding EKC concern, study findings with ARDL and nonlinear ARDL validated the EKC hypothesis for Tunisia and Morocco. Finally, the direction causality test documented unidirectional causality between renewable energy and ES, trade and ES, but the feedback hypothesis holds between EI and ES. We can advocate for specific sectoral environmental reforms in Tunisia and Morocco and suggest continuous environmentally friendly technologies by combining study findings. At the same time, subsidies on nonrenewable energy should be reduced, and green trade policies to help advance sustainable development should be implemented.
Primary Frequency Stability Support of a DFIG in Association With Pitch Angle Control
Authors: Si Xiuli , Wu Xiaoxin , You Feng , Yuan Hongliang , Xu Yien , Yang Dejian
Published: January 2022

For an electric power grid that has large penetration levels of variable renewable energy including wind generation and photovoltaics, the system frequency stability is jeopardized, which is manifest in lowering frequency nadir and settling frequency. This paper suggests an enhanced primary frequency response strategy of a doubly-fed induction generator (DFIG) in association with pitch angle control. The DFIG works in de-loaded operation with a certain reserve power via pitch angle control prior to disturbances for frequency regulation. To address this, a function of the pitch angle is employed that decreases the pitch angle with time to slowly feed the active power to the power gird. The simulation results demonstrate the effectiveness and feasibility of the proposed primary frequency response strategy including the settling frequency and frequency nadir.
Experimental Study on Fracturing Fracture Deformation Mechanism of Shale Reservoir
Authors: Xiang Zuping , Ding Yangyang , Ao Xiang , Zhong Zhicong , Li Zhijun , Xiao Hui , Chen Zhonghua , Xiao Qianhua , Ma Xinjian
Published: January 2022

After large-scale sand fracturing of horizontal wells in shale gas reservoir, fracturing fractures will deform in the production process. However, fracture deformation will lead to the decrease in fracture conductivity and then cause the decrease of gas well productivity. Therefore, in order to evaluate the fracturing fracture deformation mechanism of shale reservoirs, the shale proppant-supported fracture deformation evaluation experiments were carried out under different proppant types, particle sizes, sanding concentrations, and closure pressure conditions, respectively, and the variation curves of fracture width was calculated by a stereomicroscope under different experimental conditions. Then based on the experimental results, the fracture sensitivity factors and fracture deformation mechanism were analyzed, and the deformation mechanisms of fracturing fractures affected by proppant embedding and crushing were studied emphatically. The analysis results of fracture sensitivity factors indicate that the larger the particle size and hardness of proppant, the lower the sand concentration, proppant embedded on the shale rock surface. Moreover, the deeper the proppant is embedded, the faster the fracture conductivity decreases. In addition, the greater the closure pressure, the more serious is the proppant embedment, and the faster the fracture width decreases. The analysis results of fracture deformation mechanism show that, on the on hand, under variable closure pressure, the proppant with larger hardness and larger particle size is used for fracturing, and the proppant embedded in the fracture surface is the main cause of fracture deformation. However, if only the sand concentration of the proppant in the fracture is changed, the fracture deformation is jointly dominated by the embedding and crushing of the proppant. On the other hand, under constant closure pressure, the main mechanism of fracture deformation is that the proppant is embedded into the fracture surface when the closure pressure is low, but if the closure pressure is high, the main mechanism of fracture deformation is the crushing and compaction of proppant.
Sponge City Policy and Sustainable City Development: The Case of Shenzhen
Authors: Wang Ye , Jiang Zhe , Zhang Lin
Published: January 2022

The massive construction of buildings has changed the city’s aquatic ecological environment. The aquatic ecological condition of the city has been deteriorated with serious water issues. To coordinate various departments to jointly build the sponge city and improve the water environment, the Shenzhen government formulated the policy note on “Interim Measures for the Construction Management of Shenzhen Sponge City”. This article discusses the impact of the policy note on Shenzhen’s construction of sponge cities. The result shows that the policy is effective from the perspective of the environment. However, in the face of a complex water ecological environment, there are still some deficiencies in the policy. We therefore put forward policy suggestions for the Shenzhen municipal government to better manage its construction of sponge city in the future.
A Review of Global Policy Mechanisms Designed for Tropical Forests Conservation and Climate Risks Management
Authors: Muthee Kennedy , Duguma Lalisa , Wainaina Priscilla , Minang Peter , Nzyoka Judith
Published: January 2022

Deforestation and forest degradation of tropical forests are major global concerns due to their ecological, social, and economic roles. In the wake of climate change and its diverse global effects, fragmentation and degradation of tropical forests have jeopardized their ability to support livelihoods and regenerate climate regulating services. Concerted efforts by local, national, and international players, which are primarily scientific, technological, or economic, have borne minimal results in safeguarding these forests from destruction, necessitating a more integrated and inclusive approach. The Rio Earth Summit (1992) brought together world leaders to set targets and priorities on the global sustainability agenda and laid a strong foundation for international policy cooperation in the future. This study employed a systematic review of articles published between 1992 and 2020 to establish how various policy mechanisms have been developed and evolved to bridge forests and climate change discourse in tropical forests while highlighting their strengths and weaknesses. The initial search of peer-reviewed publications and gray literature yielded 2622 records, which were subjected to inclusion and exclusion criteria based on The Preferred Reporting Items for Systematic Review and Meta Analyses guidelines, resulting in a final list of 65 records for in-depth qualitative analysis. The study establishes that the mechanisms in place have contributed mainly to more coordination and incentives to manage climate risks, primarily through tropical forests conservation. However, hurdles such as inadequate participation and involvement of the local and indigenous people, insufficient national and local policy frameworks and bureaucracies around emissions monitoring, measuring, reporting, and verification processes continue to slow tropical forest conservation. Thus, there is a need for more integrated, multilevel, and diverse stakeholder engagement to achieve the set global targets effectively.
Biosynthesis of Antibacterial Iron-Chelating Tropolones in Aspergillus nidulans as Response to Glycopeptide-Producing Streptomycetes
Authors: Gerke Jennifer , Köhler Anna M. , Wennrich Jan-Peer , Große Verena , Shao Lulu , Heinrich Antje K. , Bode Helge B. , Chen Wanping , Surup Frank , Braus Gerhard H.
Published: January 2022

The soil microbiome comprises numerous filamentous fungi and bacteria that mutually react and challenge each other by the production of bioactive secondary metabolites. Herein, we show in liquid co-cultures that the presence of filamentous Streptomycetes producing antifungal glycopeptide antibiotics induces the production of the antibacterial and iron-chelating tropolones anhydrosepedonin (1) and antibiotic C (2) in the mold Aspergillus nidulans. Additionally, the biosynthesis of the related polyketide tripyrnidone (5) was induced, whose novel tricyclic scaffold we elucidated by NMR and HRESIMS data. The corresponding biosynthetic polyketide synthase-encoding gene cluster responsible for the production of these compounds was identified. The tropolones as well as tripyrnidone (5) are produced by genes that belong to the broad reservoir of the fungal genome for the synthesis of different secondary metabolites, which are usually silenced under standard laboratory conditions. These molecules might be part of the bacterium-fungus competition in the complex soil environment, with the bacterial glycopeptide antibiotic as specific environmental trigger for fungal induction of this cluster.