bluuebunny/crossref_metadata_2025 huggingface dataset:
Call for reimagining institutional support for PAR post-COVID
Authors: Auerbach Jeremy , Muñoz Solange , Walsh Elizabeth , Affiah Uduak , Barrera de la Torre Gerónimo , Börner Susanne , Cho Hyunji , Cofield Rachael , DiEnno Cara Marie , Graddy-Lovelace Garrett , Klassen Susanna , Limeberry Veronica , Morse Aimee , Natarajan Lucy
Published: December 2022

Although we believe academic researchers have a critical role to play in transformative systems change for social and ecological justice, we also argue that academic institutions have been (and continue to be) complicit in colonialism and in racialized, patriarchal capitalism. In this essay, we argue that if academia is to play a constructive role in supporting social and ecological resilience in the late stage Capitalocene epoch, we must move beyond mere critique to enact reimagined and decolonized forms of knowledge production, sovereignty, and structures for academic integrity. We use the pandemic as a moment of crisis to rethink what we are doing as PAR scholars and reflect on our experiences conducting PAR during the pandemic. A framework is presented for the reimaging of institutional support for the embedding of scholars in local social systems. We propose an academy with greater flexibility and consideration for PAR, one with increased funding support for community projects and community engagement offices, and a system that puts local communities first. This reimagining is followed by a set of our accounts of conducting PAR during the pandemic. Each account begins with an author's reflection on their experiences conducting PAR during the pandemic, focusing on how the current university system magnified the impacts of the pandemic. The author's reflection is then followed with a “what if” scenario were the university system changed in such a way that it mitigated or lessened the impacts of the pandemic on conducting PAR. Although this framework for a reimagined university is not a panacea, the reliance on strong in-place local teams, mutually benefiting research processes, and resources for community organizations putting in the time to collaborate with scholars can overcome many of the challenges presented by the pandemic and future crises.
PRAGMATIST: A tool to prioritize foot-and-mouth disease virus antigens held in vaccine banks
Authors: Ludi Anna B. , McLaws Melissa , Armson Bryony , Clark Jessica , Di Nardo Antonello , Parekh Krupali , Henstock Mark , Muellner Petra , Muellner Ulrich J. , Rosso Fabrizio , Prada Joaquin M. , Horton Daniel L. , Paton David J. , Sumption Keith , King Donald P.
Published: December 2022

Antigen banks have been established to supply foot-and-mouth disease virus (FMDV) vaccines at short notice to respond to incursions or upsurges in cases of FMDV infection. Multiple vaccine strains are needed to protect against specific FMDV lineages that circulate within six viral serotypes that are unevenly distributed across the world. The optimal selection of distinct antigens held in a bank must carefully balance the desire to cover these risks with the costs of purchasing and maintaining vaccine antigens. PRAGMATIST is a semi-quantitative FMD vaccine strain selection tool combining three strands of evidence: (1) estimates of the risk of incursion from specific areas (source area score); (2) estimates of the relative prevalence of FMD viral lineages in each specific area (lineage distribution score); and (3) effectiveness of each vaccine against specific FMDV lineages based on laboratory vaccine matching tests (vaccine coverage score). The output is a vaccine score, which identifies vaccine strains that best address the threats, and consequently which are the highest priority for inclusion in vaccine antigen banks. In this paper, data used to populate PRAGMATIST are described, including the results from expert elicitations regarding FMD risk and viral lineage circulation, while vaccine coverage data is provided from vaccine matching tests performed at the WRLFMD between 2011 and 2021 (n = 2,150). These data were tailored to working examples for three hypothetical vaccine antigen bank perspectives (Europe, North America, and Australia). The results highlight the variation in the vaccine antigens required for storage in these different regions, dependent on risk. While the tool outputs are largely robust to uncertainty in the input parameters, variation in vaccine coverage score had the most noticeable impact on the estimated risk covered by each vaccine, particularly for vaccines that provide substantial risk coverage across several lineages.
Elucidating genes and gene networks linked to individual susceptibility to milk fat depression in dairy goats
Authors: Suárez-Vega Aroa , Gutiérrez-Gil Beatriz , Toral Pablo G. , Frutos Pilar , Loor Juan J. , Arranz Juan-José , Hervás Gonzalo
Published: December 2022

Dietary supplementation with marine lipids modulates ruminant milk composition toward a healthier fatty acid profile for consumers, but it also causes milk fat depression (MFD). Because the dairy goat industry is mainly oriented toward cheese manufacturing, MFD can elicit economic losses. There is large individual variation in animal susceptibility with goats more (RESPO+) or less (RESPO–) responsive to diet-induced MFD. Thus, we used RNA-Seq to examine gene expression profiles in mammary cells to elucidate mechanisms underlying MFD in goats and individual variation in the extent of diet-induced MFD. Differentially expression analyses (DEA) and weighted gene co-expression network analysis (WGCNA) of RNA-Seq data were used to study milk somatic cell transcriptome changes in goats consuming a diet supplemented with marine lipids. There were 45 differentially expressed genes (DEGs) between control (no-MFD, before diet-induced MFD) and MFD, and 18 between RESPO+ and RESPO–. Biological processes and pathways such as “RNA transcription” and “Chromatin modifying enzymes” were downregulated in MFD compared with controls. Regarding susceptibility to diet-induced MFD, we identified the “Triglyceride Biosynthesis” pathway upregulated in RESPO– goats. The WGCNA approach identified 9 significant functional modules related to milk fat production and one module to the fat yield decrease in diet-induced MFD. The onset of MFD in dairy goats is influenced by the downregulation of SREBF1, other transcription factors and chromatin-modifying enzymes. A list of DEGs between RESPO+ and RESPO– goats (e.g., DBI and GPD1), and a co-related gene network linked to the decrease in milk fat (ABCD3, FABP3, and PLIN2) was uncovered. Results suggest that alterations in fatty acid transport may play an important role in determining individual variation. These candidate genes should be further investigated.
Molecular epidemiological study of Trichomonas gallinae focusing on central and southeastern Europe
Authors: Tuska-Szalay Barbara , Sipos Gábor , Takács Nóra , Kontschán Jenő , Sándor Attila D. , Péter Áron , Berta Krisztián , Kerek Ádám , Jerzsele Ákos , Votýpka Jan , Hornok Sándor
Published: December 2022

Trichomonas gallinae is a geographically widespread protozoan parasite of birds. In this study, oropharyngeal swab samples were collected in Hungary and Romania from 99 columbiform birds, including 76 feral pigeons (Columba livia domestica: 42 kept for racing, 32 with urban and two with rural habitat), four common wood pigeons (C. palumbus), 16 ring doves (Streptopelia risoria) and three Eurasian collared doves (S. decaocto). These samples were analyzed for the presence of T. gallinae using molecular methods. Racing feral pigeons had significantly higher prevalence of T. gallinae infection than urban feral pigeons. The rate of PCR-positivity was the highest among wood pigeons and ring doves. Based on 18S rRNA gene, T. gallinae was the most heterogenous among racing feral pigeons sampled in a trading-breeding place. Clinical signs were associated with only one 18S rRNA gene subtype. The most divergent 18S rRNA gene subtype, Trichomonas sp. Hu-TG37 clustered with T. canistomae and T. tenax and represents probably a new species. To our knowledge, this is the first report on the genetic diversity of T. gallinae in the southeastern European region. The results suggest that most detected T. gallinae 18S rRNA gene subtypes are not host-specific and do not cause clinical signs. The highest number of 18S rRNA gene subtypes was demonstrated among racing feral pigeons. Significantly more captive than free-living columbiform birds had T. gallinae infection. These data highlight the importance of epizootic monitoring of the genetic diversity and presence of T. gallinae in trading-breeding places of pigeons and doves.
One Health surveillance approaches for melioidosis and glanders: The Malaysian perspective
Authors: Mariappan Vanitha , Vellasamy Kumutha Malar , Anpalagar Rohan Raaj , Lim Yue-Min , Zainal Abidin Nurhamimah , Subramaniam Sreeramanan , Nathan Sheila
Published: December 2022

The One Health concept was initiated to promote the integration of human, animal, and environmental ecosystems into healthcare to ensure effective control and the sustainable governance of multifaceted health matters. Climate change, deforestation, and rigorous farming disrupt the environment, which serves as the natural habitat for many animals and microbes, increasing the likelihood of disease transmission between humans and animals. Melioidosis (neglected tropical diseases) and glanders are of humans and animals caused by the gram-negative bacteria Burkholderia pseudomallei and its close relative Burkholderia mallei, respectively. In Malaysia, although melioidosis is endemic, it is not a notifiable disease. Hence, the true prevalence of melioidosis in Malaysia is unknown and varies in different regions of the country, with reported hotspots associated with agriculture-related activities. To date, no incidence of human glanders has been reported in Malaysia, although occupational exposure for equine handlers and veterinary professionals remains a concern. Additionally, antibiotics are widely used in the healthcare and veterinary sectors to treat or prevent B. pseudomallei and B. mallei infections, leading to the emergence of resistance in B. pseudomallei. Lack of surveillance, research, assessment, and management of glanders and melioidosis is a major issue in Malaysia. Proper assessment systems and cross-discipline cooperation are vital to recognize and manage both diseases. Experts and practitioners from clinical and veterinary disciplines, environmentalists, law enforcement, policymakers, researchers, local communities, and other experts need to communicate, collaborate, and coordinate activities to fill the knowledge gap on glanders and melioidosis to reduce morbidity and mortality rates in the country. This review aims to define the organizational and functional characteristics of One Health surveillance approaches for glanders and melioidosis from a Malaysian perspective.
Aerodynamic Response of a Serpentine Inlet to Horizontal Periodic Gusts
Authors: Sun Shu , Wu Zhenlong , Huang Hexia , Bangga Galih , Tan Huijun
Published: December 2022

Gust is a common atmospheric turbulence phenomenon encountered by aircraft and is one major cause of several undesired instability problems. Although the response of aircraft to the incoming gust has been widely investigated within the subject of external-flow aerodynamics in the past decades, little attention is paid to its effects on the internal flow within aircraft engines. In this paper, a newly implemented Field Velocity Method (FVM) in OpenFOAM is used to simulate the flow field and aerodynamic responses of a serpentine inlet exposed to non-stationary horizontal sinusoidal gusts. Validations are performed on the results obtained based on the baseline Computational Fluid Dynamics (CFD) solver and the gust modeling method. Finally, the flow field and aerodynamic characteristics of the serpentine inlet under horizontal sinusoidal gust conditions are comprehensively investigated. It is found that the gusts not only significantly change the flow structure but also play an unfavorable role in the total pressure distortion of the serpentine inlet. This finding shows the necessity to consider gust effects when designing and evaluating the performance of aircraft engines.
Dissipation and Dietary Risk Assessment of Thiacloprid and Tolfenpyrad in Tea in China
Authors: Wang Weitao , Chen Hongping , Gao Di , Long Jiahuan , Long Hui , Zhang Ying
Published: December 2022

Pesticides are widely used to maintain tea yields. For achieving satisfactory effects on pests, multiple pesticides in a low application dose have been the trend at present. In this study, we investigated the dissipation and dietary risk assessment of thiacloprid and tolfenpyrad. A method for the determination of thiacloprid and tolfenpyrad was developed. The recoveries ranged from 73% to 105% with standard deviations between 0.7% and 8.3%. Limits of quantification were 0.01 mg/kg for both pesticides. Field trials were conducted in China in 2021. The half-lives were in ranges of 3.22 to 8.45 days for thiacloprid and 2.91 to 10.50 days for tolfenpyrad. The terminal residues were in the range of 0.04–2.55 mg/kg for thiacloprid and <0.01–4.00 mg/kg for tolfenpyrad, respectively. Finally, a dietary risk assessment was conducted representing the acceptable risk of the two pesticides, which of ratios were all less than 100%. The safe pre-harvest interval of 14 days was suggested. This study can serve as a guide for the rational application of thiacloprid and tolfenpyrad in tea, which also ensures the safety of human health.
Virtual New Nitrogen, Phosphorus, Water Input, and Greenhouse Gas Emission Indicators for the Potatoes Consumed in China
Authors: Gao Bing , Zhong Dongliang , Fang Xuejuan , Huang Wei , Xu Su , Cui Shenghui
Published: December 2022

Based on China’s potato staple food policy, we chose potatoes as a study case to analyze the following indicators—the virtual new nitrogen factor (VNNF), the virtual new phosphorus factor (VNPF), the virtual irrigation-water (IW) factor (VIWF), the virtual total water (IW + precipitation) factor (VTWF), and the virtual greenhouse gas (GHG) emission factor (VCF) of the potatoes consumed by households in the six potato cultivation regions—by reviewing 220 publications from 2000 to 2020. The results showed that the VNNF ranged between 17.8 ± 7.8 and 30.1 ± 17.0 kg N kg−1 N in the consumed potato, the VNPF ranged between 8.4 ± 5.0 and 18.8 ± 11.3 kg P kg−1 P in the consumed potato, the VIWF ranged between 0.3 ± 1.0 and 1.8 ± 1.4 m3 IW kg−1 for the consumed standard yield (except in the three mainly rainfed potato regions), the VTWF ranged between 4.8 ± 2.2 and 9.3 ± 3.7 m3 total water kg−1 for the consumed standard yield, and the VCF ranged between 3.4 ± 1.5 and 5.9 ± 2.4 kg CO2 equivalent kg−1 for the consumed standard yield, under the conventional practice in the six potato cultivation regions. The normalization score results indicate that the northeast, northwest, southwest, and south of China are relatively more suitable regions in which to plant potatoes, based on the VNNF, VNPF, VIWF or VTWF, and VCF indicators.
Influence of Different Irrigation Water Qualities and Irrigation Techniques on the Soil Attributes and Bacterial Community Structure
Authors: Guo Wei , Li Ping , Qi Xuebin , Hashem Mahmoud S. , Xiao Yatao , She Yingjun
Published: December 2022

Rising freshwater scarcities pose a serious threat to agricultural production. Reclaimed water (RW) is increasingly utilized as one of the alternative resources for irrigation in agriculture. Microbial communities play crucial roles in the soil microenvironment and can be used as effective indicators to assess the ecological influence of RW irrigation in soil. However, there is a lack of research on the effects of RW with different irrigation techniques on soil attributes and microbial communities. The present experiment was conducted in China to investigate the effect of two kinds of water qualities (RW and clean water (CW)), two kinds of irrigation methods (full irrigation (FI) and alternate partial root-zone irrigation (APRI)), and two kinds of irrigation techniques (furrow irrigation (FUI) and subsurface drip irrigation (SDI)) on soil chemical properties, heavy metal concentrations, and bacterial community structure. The APRI treatments received 70% of the irrigation water volume of FI. The results revealed that electrical conductivity (EC), nitrate nitrogen (NO3−-N), and heavy metal (Cu, Cd, Pb, Zn) concentrations in soil irrigated with RW were significantly higher in comparison to the soil irrigated with CW. SDI significantly decreased the contents of TN by 4.88%, the EC by 13.78%, and the heavy metal Cd concentration by 13.14% in soils than that irrigated with FUI treatment. APRI significantly decreased the heavy metal Cu concentration in soils by 6.26% compared to FI treatment. Proteobacteria, Chloroflexi, Acidobacteria, and Gemmatimonadetes in soil irrigated with RW were more abundant than that irrigated with CW. The irrigation water quality, soil moisture content, heavy metal content, TN, and EC under various irrigation techniques and methods significantly affected the structure of soil bacterial communities. In conclusion, we highlight that the SDI-APRI treatment can be an efficient irrigation practice for reducing the EC, heavy metal pollution, and the security risks of soil irrigated by RW.
The Effects of Climate Change on Heading Type Chinese Cabbage (Brassica rapa L. ssp. Pekinensis) Economic Production in South Korea
Authors: Kim Sumin , Rho Ho Young , Kim Sojung
Published: December 2022

Since Chinese cabbage is consumed fresh, its wholesale price varies with the total amount supplied on the market. However, in these days, climate variability presents a large threat to sustainable Chinese cabbage production in South Korea. To manage Chinese cabbage production well under unexpected weather conditions, it is important to study the impacts of climate variability on Chinese cabbage economic yields in South Korea. In this study, 2-year field trials were conducted in multiple locations across seven provinces in South Korea. The collected morphological data from 24 different varieties were used to develop a yield prediction model using a machine learning technique. Three Chinese cabbage groups were carried out through the clustering analysis, and a yield model was developed for each cluster group. The developed model was used to predict the cabbage economic yields under different combinations of climate change and cropping management plans. According to simulation results, Group 1 had the shortest growing degree days and produced higher yields than the other two groups. However, the overproduction of Group 1 led to a price reduction in the market of (USD(0.04–0.08) per kg), which suggested that producing Group 2 of (USD(0.31–0.96) per kg) is more beneficial to farmers. Based on the production results of the groups, their revenue varied by location and cropping management. The results of this study provide farmers with a better understanding of the relationship between production and economic benefits in future climate change scenarios.
In-Season Prediction of Corn Grain Yield through PlanetScope and Sentinel-2 Images
Authors: Li Fenling , Miao Yuxin , Chen Xiaokai , Sun Zhitong , Stueve Kirk , Yuan Fei
Published: December 2022

Crop growth and yield monitoring are essential for food security and agricultural economic return prediction. Remote sensing is an efficient technique for measuring growing season crop canopies and providing information on the spatial variability of crop yields. In this study, ten vegetation indices (VIs) derived from time series PlanetScope and Sentinel-2 images were used to investigate the potential to estimate corn grain yield with different regression methods. A field-scale spatial crop yield prediction model was developed and used to produce yield maps depicting spatial variability in the field. Results from this study clearly showed that high-resolution PlanetScope satellite data could be used to detect the corn yield variability at field level, which could explain 15% more variability than Sentinel-2A data at the same spatial resolution of 10 m. Comparison of the model performance and variable importance measure between models illustrated satisfactory results for assessing corn productivity with VIs. The green chlorophyll vegetation index (GCVI) values consistently produced the highest correlations with corn yield, accounting for 72% of the observed spatial variation in corn yield. More reliable quantitative yield estimation could be made using a multi-linear stepwise regression (MSR) method with multiple VIs. Good agreement between observed and predicted yield was achieved with the coefficient of determination value being 0.81 at 86 days after seeding. The results would help farmers and decision-makers generate predicted yield maps, identify crop yield variability, and make further crop management practices timely.
Expression Profile of Housekeeping Genes and Tissue-Specific Genes in Multiple Tissues of Pigs
Authors: Pan Xiangchun , Cai Jiali , Wang Yifei , Xu Dantong , Jiang Yao , Gong Wentao , Tian Yuhan , Shen Qingpeng , Zhang Zhe , Yuan Xiaolong , Li Jiaqi
Published: December 2022

Pigs have become an ideal model system for human disease research and development and an important farm animal that provides a valuable source of nutrition. To profile the all-sided gene expression and their biological functions across multiple tissues, we conducted a comprehensive analysis of gene expression on a large scale around the side of housekeeping genes (HKGs), tissue specific genes (TSGs), and the co-expressed genes in 14 various tissues. In this study, we identified 2351 HKGs and 3018 TSGs across tissues, among which 4 HKGs (COX1, UBB, OAZ1/NPFF) exhibited low variation and high expression levels, and 31 particular TSGs (e.g., PDC, FKBP6, STAT2, and COL1A1) were exclusively expressed in several tissues, including endocrine brain, ovaries, livers, backfat, jejunum, kidneys, lungs, and longissimus dorsi muscles. We also obtained 17 modules with 230 hub genes (HUBGs) by weighted gene co-expression network analysis. On the other hand, HKGs functions were enriched in the signaling pathways of the ribosome, spliceosome, thermogenesis, oxidative phosphorylation, and nucleocytoplasmic transport, which have been highly suggested to involve in the basic biological tissue activities. While TSGs were highly enriched in the signaling pathways that were involved in specific physiological processes, such as the ovarian steroidogenesis pathway in ovaries and the renin-angiotensin system pathway in kidneys. Collectively, these stable, specifical, and co-expressed genes provided useful information for the investigation of the molecular mechanism for an understanding of the genetic and biological processes of complex traits in pigs.
Signaling Lymphocytic Activation Molecule Family Member 1 Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication
Authors: Song Haofei , Quan Jima , Li Chang , Liang Wan , Zhang Lan , Wang Shuangshuang , Lu Hongyu , Yang Keli , Zhou Danna , Li Peng , Tian Yongxiang
Published: December 2022

The porcine reproductive and respiratory syndrome virus (PRRSV) causes a highly contagious disease in domestic swine. Signaling lymphocytic activation molecule family member 1 (SLAMF1) is a costimulatory factor that is involved in innate immunity, inflammation, and infection. Here, we demonstrate that overexpression of the SLAMF1 gene inhibited PRRSV replication significantly and reduced the levels of key signaling pathways, including MyD88, RIG-I, TLR2, TRIF, and inflammatory factors IL-6, IL-1β, IL-8, TNF-β, TNF-α, and IFN-α in vitro. However, the knockdown of the SLAMF1 gene could enhance replication of the PRRSV and the levels of key signaling pathways and inflammatory factors. Overall, our results identify a new, to our knowledge, antagonist of the PRRSV, as well as a novel antagonistic mechanism evolved by inhibiting innate immunity and inflammation, providing a new reference and direction for PRRSV disease resistance breeding.
Effects of Dietary Supplementation of Lactobacillus acidophilus on Blood Parameters and Gut Health of Rabbits
Authors: Colombino Elena , Biasato Ilaria , Michetti Alberta , Rubino Maria Gabriella , Franciosa Irene , Giribaldi Marzia , Antoniazzi Sara , Bergagna Stefania , Paliasso Giulia , Ferrocino Ilario , Cavallarin Laura , Gasco Laura , Capucchio Maria Teresa
Published: December 2022

This study aimed to evaluate the effects of Lactobacillus acidophilus D2/CSL (L-1 × 109 cfu/kg feed/day) on biochemical parameters, faecal score (FS), cecal pH, gut morphometry, microbiota and cecal short-chain fatty acid (SCFAs) in rabbits. Three zootechnical trials were performed and in each trial 30 rabbits were allotted to two groups; a probiotic group (L) and a control group (C). At slaughter (day 45), samples of blood, duodenum, jejunum, ileum, liver and spleen were collected and submitted to histomorphometric analyses. Blood biochemical analyses, cecal microbiota and SCFAs determination were also performed. In trial 1 and 3, L. acidophilus D2/CSL did not affect productive parameters (p > 0.05). However, L group of trial 1 showed a lower morbidity and mortality compared to the control. In trial 2, C group showed a higher daily feed intake (p = 0.018) and a positive statistical tendency for live weight and average daily gain (p = 0.068). On the contrary, albumin was higher and ALFA-1 globulin was lower in the C group compared to L (p < 0.05). In all the trials, FS, cecal pH, histomorphometry, microbiota and SCFAs were unaffected. In conclusion, L. acidophilus D2/CSL did not impair growth performances, gut and rabbit’s health, reducing morbidity and mortality.
Beef Cattle Preference and Usage of Environmental Enrichments Provided Simultaneously in a Pasture-Based Environment
Authors: Dickson Emily J. , Campbell Dana L. M. , Lee Caroline , Lea Jim M. , McDonald Paul G. , Monk Jessica E.
Published: December 2022

Environmental enrichment can improve livestock welfare through increasing environmental complexity to promote a greater range of natural behaviours. However, there is limited understanding of the need for and impacts of enrichments for extensively managed beef cattle that can sometimes be kept in grassed paddocks devoid of additional natural and artificial features, i.e., ‘barren pastures’. This trial assessed which enrichments beef cattle preferred and utilised in a barren paddock environment. Eight groups of seven Angus steers housed on pastured paddocks devoid of natural or artificial features were observed during daylight hours for two days a week over a period of three weeks, after being presented with four enrichments simultaneously: a cattle brush, a piece of hanging rope, a tree stump, and a woodchip pile. Although enrichment use generally decreased over time, the brush, stump, and woodchip maintained a higher level of use than the rope, based on the frequency of interactions and number of displacements around the enrichments (both p < 0.001). This suggests that the brush, stump, and woodchip pile were more valuable resources to the cattle, allowing for grooming and lying behaviours, although oral manipulations also occurred on the stump, woodchip, and rope. The inclusion of these enrichments can increase the complexity of barren pasture environments and allow for the increased expression of natural behaviours, potentially contributing to improved welfare.
In Vitro Assessment of Antimicrobial Activity of Phytobiotics Composition towards of Avian Pathogenic Escherichia coli (APEC) and Other E. coli Strains Isolated from Broiler Chickens
Authors: Chodkowska Karolina A. , Iwiński Hubert , Wódz Karolina , Nowak Tomasz , Różański Henryk
Published: December 2022

Escherichia coli infections (including APEC) in broiler chickens are not only a health and economic problem of the flock, but also a significant health threat to poultry meat consumers. The prophylactic and therapeutic effects of the phytobiotic composition on E. coli in broiler chickens were previously described. However, most of the data were related to the reference strains (for both in vitro and in vivo models). Based on the previous studies in human and animals, E. coli strains seem to be multidrug resistance. This, in turn, makes it necessary to develop effective alternative methods of treating this type of infection already at the stage of poultry production. In the present study, the antibacterial activity against various strains of E. coli (including APEC) was assessed for two innovative phytobiotics mixtures: H1, containing thymol, menthol, linalool, trans-anethole, methyl salicylate, 1,8-cineol, and p-cymene; H2, in addition to compounds from H1, containing terpinen-4-ol and γ-terpinene. The unique mixtures of phytobiotics used in the experiment were effective against various strains of E. coli, also against APEC, isolated from broiler chickens from traditional industrial breeding, as well as against those showing colistin resistance. The minimum inhibitory concentration (MIC) values for these unique mixtures were: For H1 1:512 for APEC and non-APEC E. coli strains isolated from day old chicks (DOCs), 1:512 for non-APEC, and 1:1024 for non-APEC isolated from broilers sample. For mixture H2, MIC for APEC from both type of samples (DOCs and broilers) was 1:1024 and for non-APEC (DOCs and broilers) was 1:512. The results suggest that phytobiotic compositions used in this study can be successfully used as a natural alternative to antibiotics in the treatment of E. coli infections in broiler chickens. The promising results may be a crucial point for further analyses in broiler flocks exposed to E. coli infections and where it is necessary to reduce the level of antibiotics or completely eliminate them, thus reducing the risk of foodborne infections.
Fabrication and Evaluation of Basil Essential Oil-Loaded Halloysite Nanotubes in Chitosan Nanocomposite Film and Its Application in Food Packaging
Authors: Chaudhary Narayan , Mishra Gourav , Yadav Tushar , Srivastava Nishant , Maurya Vimal K. , Saxena Shailendra K.
Published: December 2022

Increasing health concerns regarding the use of plasticware have led to the development of ecofriendly biodegradable packaging film from natural polymer and food additives. In the present study, basil essential oil (BEO) loaded halloysite nanotubes (HNTs) composite films were synthesized using a solution casting method. The effects of BEO and nanotube concentration on the mechanical, physical, structural, barrier, and antioxidant properties of films were evaluated. Scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transform infrared (FTIR) demonstrated well-dispersed HNTs and BEO in tailored composite films. The addition of BEO in Chitosan (Ch) film caused darkening of the film color; furthermore, the incorporation of HNTs in varied concentrations increased opaqueness in Ch/BEO film. The Ch/BEO film, upon adding HNTs 5–30 wt%, exhibited a corresponding increase in the film thickness (0.108–0.135 mm) when compared with the Ch/BEO film alone (0.081 mm). The BEO-loaded HNTs composite films displayed reduced moisture content and characteristic barrier and UV properties. The Ch/BEO film with 15 wt% HNTs was found to have enhanced antioxidant activity. The Ch/BEO/HNTs composite also managed to prevent broccoli florets from losing weight and firmness during storage. The enhanced barrier and antioxidant qualities of the nanocomposite film suggest its potential application in the food processing and packaging sector. This is the first ever report on the fabrication of nanocomposite film using BEO and HNTs for food packaging. The low production cost and ecofriendly approach make the film acceptable for further research and commercialization thereafter.
L-carnosine Attenuates Bleomycin-Induced Oxidative Stress via NFκB Pathway in the Pathogenesis of Pulmonary Fibrosis
Authors: Park Jaehyun , Jang Jimin , Cha Sang-Ryul , Baek Hyosin , Lee Jooyeon , Hong Seok-Ho , Lee Hyang-Ah , Lee Tae-Jin , Yang Se-Ran
Published: December 2022

Idiopathic Pulmonary fibrosis (IPF), a chronic interstitial lung disease, has pulmonary manifestations clinically characterized by collagen deposition, epithelial cell injury, and a decline in lung function. L-carnosine, a dipeptide consisting of β-alanine and L-histidine, has demonstrated a therapeutic effect on various diseases because of its pivotal function. Despite the effect of L-carnosine in experimental IPF mice, its anti-oxidative effect and associated intercellular pathway, particularly alveolar epithelial cells, remain unknown. Therefore, we demonstrated the anti-fibrotic and anti-inflammatory effects of L-carnosine via Reactive oxygen species (ROS) regulation in bleomycin (BLM)-induced IPF mice. The mice were intratracheally injected with BLM (3 mg/kg) and L-carnosine (150 mg/kg) was orally administrated for 2 weeks. BLM exposure increased the protein level of Nox2, Nox4, p53, and Caspase-3, whereas L-carnosine treatment suppressed the protein level of Nox2, Nox4, p53, and Caspase-3 cleavage in mice. In addition, the total SOD activity and mRNA level of Sod2, catalase, and Nqo1 increased in mice treated with L-carnosine. At the cellular level, a human fibroblast (MRC-5) and mouse alveolar epithelial cell (MLE-12) were exposed to TGFβ1 following L-carnosine treatment to induce fibrogenesis. Moreover, MLE-12 cells were exposed to cigarette smoke extract (CSE). Consequently, L-carnosine treatment ameliorated fibrogenesis in fibroblasts and alveolar epithelial cells, and inflammation induced by ROS and CSE exposure was ameliorated. These results were associated with the inhibition of the NFκB pathway. Collectively, our data indicate that L-carnosine induces anti-inflammatory and anti-fibrotic effects on alveolar epithelial cells against the pathogenesis of IPF.
Neuronal Oxidative Stress Promotes α-Synuclein Aggregation In Vivo
Authors: Won Seok Joon , Fong Rebecca , Butler Nicholas , Sanchez Jennifer , Zhang Yiguan , Wong Candance , Tambou Nzoutchoum Olive , Huynh Annie , Pan June , Swanson Raymond A.
Published: December 2022

Both genetic and environmental factors increase risk for Parkinson’s disease. Many of the known genetic factors influence α-synuclein aggregation or degradation, whereas most of the identified environmental factors produce oxidative stress. Studies using in vitro approaches have identified mechanisms by which oxidative stress can accelerate the formation of α-synuclein aggregates, but there is a paucity of evidence supporting the importance of these processes over extended time periods in brain. To assess this issue, we evaluated α-synuclein aggregates in brains of three transgenic mouse strains: hSyn mice, which overexpress human α-synuclein in neurons and spontaneously develop α-synuclein aggregates; EAAT3−/− mice, which exhibit a neuron-specific impairment in cysteine uptake and resultant neuron-selective chronic oxidative stress; and double-transgenic hSyn/EAAT3−/− mice. Aggregate formation was evaluated by quantitative immunohistochemistry for phosphoserine 129 α-synuclein and by an α-synuclein proximity ligation assay. Both methods showed that the double transgenic hSyn/EAAT3−/− mice exhibited a significantly higher α-synuclein aggregate density than littermate hSyn mice in each brain region examined. Negligible aggregate formation was observed in the EAAT3−/− mouse strain, suggesting a synergistic rather than additive interaction between the two genotypes. A similar pattern of results was observed in assessments of motor function: the pole test and rotarod test. Together, these observations indicate that chronic, low-grade neuronal oxidative stress promotes α-synuclein aggregate formation in vivo. This process may contribute to the mechanism by which environmentally induced oxidative stress contributes to α-synuclein pathology in idiopathic Parkinson’s disease.
The Role of Lead and Cadmium in Gynecological Malignancies
Authors: Furtak Gabriela , Kozłowski Mateusz , Kwiatkowski Sebastian , Cymbaluk-Płoska Aneta
Published: December 2022

Lead and cadmium are non-essential and toxic heavy metals. Their presence and elevated levels can lead to many pathologies. They disrupt the antioxidant properties of many enzymes, consume the resources of antioxidant cells, and thus participate in the generation of oxidative stress, which may result in DNA damage. In addition, they have been found to be carcinogenic through their genotoxic properties. They have been shown to be present in various types of cancer, including cancer of the female reproductive system. Both metals have been recognized as metalloestrogens, which are important in hormone-related cancers. Participation in the oncogenesis of ovarian, endometrial and cervical cancer was analysed in detail, using the available research in this field. We emphasize their role as potential biomarkers in cancer risk and diagnosis as well as advancement of gynaecological malignancies.
Linear Active Disturbance Rejection Control-Based Diagonal Recurrent Neural Network for Radar Position Servo Systems with Dead Zone and Friction
Authors: Cui Shuai , Zhu Guixin , Zhao Tong
Published: December 2022

This paper proposes a control scheme for the radar position servo system facing dead zone and friction nonlinearities. The controller consists of the linear active disturbance rejection controller (LADRC) and diagonal recurrent neural network (DRNN). The LADRC is designed to estimate in real time and compensate for the disturbance with vast matched and mismatched uncertainties, including the internal dead zone and friction nonlinearities and external noise disturbance. The DRNN is introduced to optimize the parameters in the linear state error feedback (LSEF) of the LADRC in real time and estimate the model information, namely Jacobian information, of the plant on-line. In addition, considering the Cauchy distribution, an adaptive tracking differentiator (ATD) is designed in order to manage the contradiction between filtering performance and tracking speed, which is introduced to the LADRC. Another novel idea is that the back propagation neuron network (BPNN) is also introduced to tune the parameters of the LADRC, just as in the DRNN, and the comparison results show that the DRNN is more suitable for high precision control due to its feedback structure compared with the static BPNN. Moreover, the regular controller performances and robust performance of the proposed control approach are verified based on the radar position servo system by MATLAB simulations.
A Review of Applications of Nanocellulose to Preserve and Protect Cultural Heritage Wood, Paintings, and Historical Papers
Authors: Fornari Anastasia , Rossi Marco , Rocco Daniele , Mattiello Leonardo
Published: December 2022

Due to several of their characteristics, such as their renewability, sustainability, and eco-friendliness, nanocellulose-based materials are arousing growing interest from researchers in various fields of study and applications. The purpose of this review article is to provide an overall view of the most recent applications of these innovative bio-nanomaterials in the field of cultural heritage. First, an introduction of the different classes of cellulose nanomaterials and their synthesis and characterization methods is presented. After that, many consolidation treatments based on nanocellulose structures for the recovery of degraded and archeological wood, the stabilization of damaged painting canvases, and the deacidification of historical papers are shown in order to underline the advanced potential of nanocellulose for the conservation of artistic heritage and the respect for the environment.
Special Issue “Extreme Sciences and Engineering II”
Authors: Kaewunruen Sakdirat
Published: December 2022

Disruptions in the operations of our countries’ infrastructures can undermine the function of our societies and their economies [...]
Emotionally Controllable Talking Face Generation from an Arbitrary Emotional Portrait
Authors: Zhao Zikang , Zhang Yujia , Wu Tianjun , Guo Hao , Li Yao
Published: December 2022

With the continuous development of cross-modality generation, audio-driven talking face generation has made substantial advances in terms of speech content and mouth shape, but existing research on talking face emotion generation is still relatively unsophisticated. In this work, we present Emotionally Controllable Talking Face Generation from an Arbitrary Emotional Portrait to synthesize lip-sync and an emotionally controllable high-quality talking face. Specifically, we take a facial reenactment perspective, using facial landmarks as an intermediate representation driving the expression generation of talking faces through the landmark features of an arbitrary emotional portrait. Meanwhile, decoupled design ideas are used to divide the model into three sub-networks to improve emotion control. They are the lip-sync landmark animation generation network, the emotional landmark animation generation network, and the landmark-to-animation translation network. The two landmark animation generation networks are responsible for generating content-related lip area landmarks and facial expression landmarks to correct the landmark sequences of the target portrait. Following this, the corrected landmark sequences and the target portrait are fed into the translation network to generate an emotionally controllable talking face. Our method controls the expressions of talking faces by driving the emotional portrait images while ensuring the generation of animated lip-sync, and can handle new audio and portraits not seen during training. A multi-perspective user study and extensive quantitative and qualitative evaluations demonstrate the superiority of the system in terms of visual emotion representation and video authenticity.
XtoE: A Novel Constructive and Camouflaged Adaptive Data Hiding and Image Encryption Scheme for High Dynamic Range Images
Authors: Lan Chi-Feng , Wang Chung-Ming , Lin Woei
Published: December 2022

High dynamic range (HDR) image data hiding and encryption has attracted much interest in recent years due the benefits of providing high quality realistic images and versatile applications, such as copyright protection, data integrity, and covert communication. In this paper, we propose a novel constructive and camouflaged adaptive data hiding and image encryption scheme for HDR images. Our algorithm disguises hidden messages when converting an original OpenEXR format to the RGBE encoding, which contains the Red, Green, and Blue color channels and an exponent E channel. During the conversion process, we determine an optimal base for each pixel by considering the user’s demands and the exponent E channel information to achieve adaptive message concealment. To prevent inappropriate access to the stego image, we perform the bit-level permutation and confusion using a 2D Sine Logistic modulation map with hyperchaotic behavior and a random permutation scheme with the time complexity of ON. To the best of our knowledge, our algorithm is the first in HDR data hiding literature able to predict the image distortion and satisfy a user’s request for the embedding capacity. Our algorithm offers 18% to 32% larger embedding rate than that provided by the current state-of-the-art works without degrading the quality of the stego image. Experimental results confirm that our scheme provides high security superior to the competitors.
Anthropomorphic Grasping of Complex-Shaped Objects Using Imitation Learning
Authors: Yi Jae-Bong , Kim Joonyoung , Kang Taewoong , Song Dongwoon , Park Jinwoo , Yi Seung-Joon
Published: December 2022

This paper presents an autonomous grasping approach for complex-shaped objects using an anthropomorphic robotic hand. Although human-like robotic hands have a number of distinctive advantages, most of the current autonomous robotic pickup systems still use relatively simple gripper setups such as a two-finger gripper or even a suction gripper. The main difficulty of utilizing human-like robotic hands lies in the sheer complexity of the system; it is inherently tough to plan and control the motions of the high degree of freedom (DOF) system. Although data-driven approaches have been successfully used for motion planning of various robotic systems recently, it is hard to directly apply them to high-DOF systems due to the difficulty of acquiring training data. In this paper, we propose a novel approach for grasping complex-shaped objects using a high-DOF robotic manipulation system consisting of a seven-DOF manipulator and a four-fingered robotic hand with 16 DOFs. Human demonstration data are first acquired using a virtual reality controller with 6D pose tracking and individual capacitive finger sensors. Then, the 3D shape of the manipulation target object is reconstructed from multiple depth images recorded using the wrist-mounted RGBD camera. The grasping pose for the object is estimated using a residual neural network (ResNet), K-means clustering (KNN), and a point-set registration algorithm. Then, the manipulator moves to the grasping pose following the trajectory created by dynamic movement primitives (DMPs). Finally, the robot performs one of the object-specific grasping motions learned from human demonstration. The suggested system is evaluated by an official tester using five objects with promising results.
Multi-Level Knowledge-Aware Contrastive Learning Network for Personalized Recipe Recommendation
Authors: Bai Zijian , Huang Yinfeng , Zhang Suzhi , Li Pu , Chang Yuanyuan , Lin Xiang
Published: December 2022

Personalized recipe recommendation is attracting more and more attention, which can help people make choices from the exploding growth of online food information. Unlike other recommendation tasks, the target of recipe recommendation is a non-atomic item, so attribute information is especially important for the representation of recipes. However, traditional collaborative filtering or content-based recipe recommendation methods tend to focus more on user–recipe interaction information and ignore higher-order semantic and structural information. Recently, graph neural networks (GNNs)-based recommendation methods provided new ideas for recipe recommendation, but there was a problem of sparsity of supervised signals caused by the long-tailed distribution of heterogeneous graph entities. How to construct high-quality representations of users and recipes becomes a new challenge for personalized recipe recommendation. In this paper, we propose a new method, a multi-level knowledge-aware contrastive learning network (MKCLN) for personalized recipe recommendation. Compared with traditional comparative learning, we design a multi-level view to satisfy the requirement of fine-grained representation of users and recipes, and use multiple knowledge-aware aggregation methods for node fusion to finally make recommendations. Specifically, the local-level includes two views, interaction view and semantic view, which mine collaborative information and semantic information for high-quality representation of nodes. The global-level learns node embedding by capturing higher-order structural information and semantic information through a network structure view. Then, a kind of self-supervised cross-view contrastive learning is invoked to make the information of multiple views collaboratively supervise each other to learn fine-grained node embeddings. Finally, the recipes that satisfy personalized preferences are recommended to users by joint training and model prediction functions. In this study, we conduct experiments on two real recipe datasets, and the experimental results demonstrate the effectiveness and advancement of MKCLN.