bluuebunny/crossref_metadata_2025 huggingface dataset:
Hydroxy-Propil-β-Cyclodextrin Inclusion Complexes of two Biphenylnicotinamide Derivatives: Formulation and Anti-Proliferative Activity Evaluation in Pancreatic Cancer Cell Models
Authors: Iacobazzi Rosa Maria , Cutrignelli Annalisa , Stefanachi Angela , Porcelli Letizia , Lopedota Angela Assunta , Di Fonte Roberta , Lopalco Antonio , Serratì Simona , Laquintana Valentino , Silvestris Nicola , Franco Massimo , Cellamare Saverio , Leonetti Francesco , Azzariti Amalia , Denora Nunzio
Published: September 2020

Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive malignancies, with poor outcomes largely due to its unique microenvironment, which is responsible for the low response to drugs and drug-resistance phenomena. This clinical need led us to explore new therapeutic approaches for systemic PDAC treatment by the utilization of two newly synthesized biphenylnicotinamide derivatives, PTA73 and PTA34, with remarkable antitumor activity in an in vitro PDAC model. Given their poor water solubility, inclusion complexes of PTA34 and PTA73 in Hydroxy-Propil-β-Cyclodextrin (HP-β-CD) were prepared in solution and at the solid state. Complexation studies demonstrated that HP-β-CD is able to form stable host–guest inclusion complexes with PTA34 and PTA73, characterized by a 1:1 apparent formation constant of 503.9 M−1 and 369.2 M−1, respectively (also demonstrated by the Job plot), and by an increase in aqueous solubility of about 150 times (from 1.95 µg/mL to 292.5 µg/mL) and 106 times (from 7.16 µg/mL to 762.5 µg/mL), in the presence of 45% w/v of HP-β-CD, respectively. In vitro studies confirmed the high antitumor activity of the complexed PTA34 and PTA73 towards PDAC cells, the strong G2/M phase arrest followed by induction of apoptosis, and thus their eligibility for PDAC therapy.
Evaluation of Organic Wastes as Substrates for Rearing Zophobas morio, Tenebrio molitor, and Acheta domesticus Larvae as Alternative Feed Supplements
Authors: Harsányi Endre , Juhász Csaba , Kovács Elza , Huzsvai László , Pintér Richárd , Fekete György , Varga Zsolt István , Aleksza László , Gyuricza Csaba
Published: September 2020

Studies have focused on identifying combinations of insects and organic waste to optimise bio-conversion. Here, the effects of different diets (10% chicken feed complemented with 90% vegetable waste, garden waste, cattle manure, or horse manure) on growth and survival rates, and nutritional value of Zophobas morio and Tenebrio molitor larvae, and Acheta domesticus were investigated. Compared with chicken feed, organic waste decreased the individual larval weight, although green waste showed fewer negative effects than the manure. The macronutrient concentrations in garden waste were moderate compared with chicken feed, and vegetable waste was the poorest diet in terms of nutrient concentration, as revealed by the principal component analysis (PCA). There was no difference in weight between larvae reared on garden waste and those reared on vegetable waste. Tenebrio molitor and A. domesticus showed the maximum growth rates at 71–101 and 36–66 days of age at 22.5 ± 2.5 °C, respectively. The PCA showed that the protein and fibre concentrations were inversely proportional to fat concentration in the larvae. Acheta domesticus was rich in proteins, whereas Z. morio and T. molitor were rich in fat. Feeding nutrient-poor diets resulted in a lower protein and a higher fat concentration in the larvae.
Hematological Phenotype of COVID-19-Induced Coagulopathy: Far from Typical Sepsis-Induced Coagulopathy
Authors: Umemura Yutaka , Yamakawa Kazuma , Kiguchi Takeyuki , Nishida Takeshi , Kawada Masahiro , Fujimi Satoshi
Published: September 2020

Background: Blood coagulation disorders commonly occur with severe coronavirus disease 2019 (COVID-19). However, there is only limited evidence on differentiating the pattern of the hemostatic parameters from those of typical sepsis-induced coagulopathy (SIC). Methods: To elucidate the specific pattern of coagulopathy induced by COVID-19 pneumonia, this retrospective, observational study targeted consecutive adult patients with COVID-19-induced acute respiratory distress syndrome (ARDS) and compared hemostatic biomarkers with non-COVID-19-induced septic ARDS. Multilevel mixed-effects regression analysis was performed and Kaplan–Meier failure curves were constructed. Results: We enrolled 24 patients with COVID-19-induced ARDS and 200 patients with non-COVID-19-induced ARDS. Platelet count, antithrombin activity, and prothrombin time in the COVID-19 group were almost within normal range and time series alterations of these markers were significantly milder than the non-COVID-19 group (p = 0.052, 0.037, and 0.005, respectively). However, fibrin/fibrinogen degradation product and D-dimer were significantly higher in the COVID-19 group (p = 0.001, 0.002, respectively). COVID-19 patients had moderately high levels of thrombin–antithrombin complex and plasmin-alpha2-plasmin inhibitor complex but normal plasminogen activator inhibitor-1 level. Conclusions: The hematological phenotype of COVID-19-induced coagulopathy is quite different from that in typical SIC characterized by systemic hypercoagulation and suppressed fibrinolysis. Instead, local thrombus formation might be promoted in severe COVID-19.
Analysis of miR-29 Serum Levels in Patients with Neuroendocrine Tumors—Results from an Exploratory Study
Authors: Özdirik Burcin , Stueven Anna K. , Mohr Raphael , Geisler Lukas , Wree Alexander , Knorr Jana , Demir Münevver , Vucur Mihael , Loosen Sven H. , Benz Fabian , Reiss Markus , Wiedenmann Bertram , Tacke Frank , Jann Henning , Hellberg Teresa , Roderburg Christoph
Published: September 2020

Background and aims: Due to its involvement in tumor biology as well as tumor-associated stroma cell responses, recent data suggested a potential role of miR-29 as a biomarker for different malignancies. However, its role in neuroendocrine tumors (NETs) is only poorly understood. Methods: We measured circulating levels of miR-29b in 45 patients with NET and compared them to 19 healthy controls. Results were correlated with clinical records. Results: In our cohort of NET patients treated between 2010 and 2019 at our department, miR-29b serum levels were significantly downregulated when compared to healthy control samples. Further, a significant correlation between chromogranin A (CgA) and relative miR-29b levels was noted. However, serum levels of miR-29b were independent of tumor-related factors such as proliferation activity according to Ki-67 index, tumor grading, the TMN stage of malignant tumors, somatostatin receptor expression or clinical features such as functional or non-functional disease and presence of tumor relapse. Finally, in contrast to previous results from other malignancies, miR-29b serum levels were not a significant predictor of overall survival in NET patients. Conclusion: Our data suggest a role for miR-29b serum levels as a previously unrecognized biomarker for diagnosis of NET. However, miR-29 does not allow for predicting tumor stage or patients’ outcome.
Fetal Cardiac Interventions—Polish Experience from “Zero” to the Third World Largest Program
Authors: Debska Marzena , Kolesnik Adam , Rebizant Beata , Sekowska Agnieszka , Grzyb Agnieszka , Chaberek Katarzyna , Witwicki Jacek , Debski Romuald , Dangel Joanna
Published: September 2020

This article presents the technical aspects of the Polish fetal cardiac interventions (FCI) program, including preparation of the team and modifications in the technique of the procedure that aim to increase its safety for the mother and the fetus. Over 9 years, 128 FCI in 113 fetuses have been performed: 94 balloon aortic valvuloplasties (fBAV), 14 balloon atrioseptoplasties (fBAS) with stent (BAS+), 5 balloon atrioseptoplasties without stent placement (BAS−), and 15 fetal pulmonary valvuloplasties (fBPS). The technical success rate ranged from 80% (BAS−) to 89% (fBAV), while the procedure-related death rate (defined as death within 72 hours following the procedure) ranged from 7% (fBAV and fBPV) to 20% (BAS). There were 98 live births after all FCI (3 pregnancies continue). Median gestational age at delivery was 39 weeks in our center and 38 weeks in other centers.
Numerical Simulation of a Polar Ship Moving in Level Ice Based on a One-Way Coupling Method
Authors: Ni Bao-Yu , Chen Zi-Wang , Zhong Kai , Li Xin-Ang , Xue Yan-Zhuo
Published: September 2020

In most previous ice–ship interaction studies involving fluid effects, ice was taken as unbreakable. Building breakable level ice on water domain is still a big challenge in numerical simulation. This paper overcomes this difficulty and presents a numerical modeling of a ship moving in level ice on the water by using a one-way CFD-DEM (computational fluid dynamics-discrete element method) coupling method. The detailed numerical processes and techniques are introduced. The ice crack propagation process including radial and circular cracks have been observed. Numerical results are compared with previous experimental data and good agreement has been achieved. The results show that water resistance is an order of magnitude smaller than ice resistance during the ice-breaking process. Ice resistance shows strong oscillation along with ice failure process, which are affected by ship speed and ice thickness significantly.
The Other Side of Alzheimer’s Disease: Influence of Metabolic Disorder Features for Novel Diagnostic Biomarkers
Authors: Argentati Chiara , Tortorella Ilaria , Bazzucchi Martina , Emiliani Carla , Morena Francesco , Martino Sabata
Published: September 2020

Nowadays, the amyloid cascade hypothesis is the dominant model to explain Alzheimer’s disease (AD) pathogenesis. By this hypothesis, the inherited genetic form of AD is discriminated from the sporadic form of AD (SAD) that accounts for 85–90% of total patients. The cause of SAD is still unclear, but several studies have shed light on the involvement of environmental factors and multiple susceptibility genes, such as Apolipoprotein E and other genetic risk factors, which are key mediators in different metabolic pathways (e.g., glucose metabolism, lipid metabolism, energetic metabolism, and inflammation). Furthermore, growing clinical evidence in AD patients highlighted the presence of affected systemic organs and blood similarly to the brain. Collectively, these findings revise the canonical understating of AD pathogenesis and suggest that AD has metabolic disorder features. This review will focus on AD as a metabolic disorder and highlight the contribution of this novel understanding on the identification of new biomarkers for improving an early AD diagnosis.
Conserved Patterns in Developmental Processes and Phases, Rather than Genes, Unite the Highly Divergent Bilateria
Authors: Ferretti Luca , Krämer-Eis Andrea , Schiffer Philipp H.
Published: September 2020

Bilateria are the predominant clade of animals on Earth. Despite having evolved a wide variety of body plans and developmental modes, they are characterized by common morphological traits. By default, researchers have tried to link clade-specific genes to these traits, thus distinguishing bilaterians from non-bilaterians, by their gene content. Here we argue that it is rather biological processes that unite Bilateria and set them apart from their non-bilaterian sisters, with a less complex body morphology. To test this hypothesis, we compared proteomes of bilaterian and non-bilaterian species in an elaborate computational pipeline, aiming to search for a set of bilaterian-specific genes. Despite the limited confidence in their bilaterian specificity, we nevertheless detected Bilateria-specific functional and developmental patterns in the sub-set of genes conserved in distantly related Bilateria. Using a novel multi-species GO-enrichment method, we determined the functional repertoire of genes that are widely conserved among Bilateria. Analyzing expression profiles in three very distantly related model species—D. melanogaster, D. rerio and C. elegans—we find characteristic peaks at comparable stages of development and a delayed onset of expression in embryos. In particular, the expression of the conserved genes appears to peak at the phylotypic stage of different bilaterian phyla. In summary, our study illustrate how development connects distantly related Bilateria after millions of years of divergence, pointing to processes potentially separating them from non-bilaterians. We argue that evolutionary biologists should return from a purely gene-centric view of evolution and place more focus on analyzing and defining conserved developmental processes and periods.
Microstructures and Properties of AlMgTi-Based Metal-Intermetallic Laminate Composites by Dual-Steps Vacuum Hot Pressing
Authors: Meng Linggang , Zhou Bingwen , Ya Bin , Jing Dong , Jiang Yingxi , Zhang Danning , Zhang Xingguo
Published: September 2020

AlMgTi-based metal–intermetallic laminated composites were successfully fabricated through an innovative dual-step vacuum hot pressing. First, this study prepares the AlTi-based laminated composites by vacuum hot pressing at 650 °C. Then, the researchers place the Mg-Al-1Zn (AZ31) magnesium alloy between the prepared AlTi-based laminated composites at 430 °C for hot pressing. This study investigates the microstructure, phase composition, and microhardness distribution across interfaces of the intermetallics and metal. A multilayer phase (Mg17Al12, Al3Mg2, and transition layers) structure can be found from the diffusion layers between Al and AZ31. The microhardness of the material presents a wavy distribution in the direction perpendicular to the layers; the maximum can be up to 600.0 HV0.2 with a minimum of 28.7 HV0.2 The microhardness gradient of an AlMgTi-based composite is smoother due to the different microhardness of the layers, and reduces the interface stress concentration. The bending strength of AlMgTi-based composites can reach 265 MPa, and the specific strength is 105 × 103 Nm/kg, higher than AlTi-based composites.
Enhanced Morphology-Dependent Tensile Property and Breakdown Strength of Impact Polypropylene Copolymer for Cable Insulation
Authors: Yang Kai , Liu Yun , Yan Zhimin , Tian Ye , Liu Yitao , Jing Zhenghong , Li Jianying , Li Shengtao
Published: September 2020

The decrease in electrical properties caused by the toughening of polypropylene (PP) is a difficult problem for the modification of PP used for cable insulation. In this research, an isotactic PP, a cross-linked polyethylene (XLPE) and two impact PP copolymers (IPCs) with an ethylene–propylene rubber phase content of 15 and 30% were prepared to assess the possibility of IPCs to be used as cable insulating material. The tensile properties and breakdown strength were evaluated, meanwhile, the rubber phase content dependence of the crystalline structure, morphology and trap distribution were also investigated. Results show that IPCs with a 15% rubber phase content (IPC15) can achieve the simultaneous improvement of elongation at break and breakdown strength compared with isotactic PP, which can be attributed to the special crystalline structure. According to the results of differential scanning calorimetry (DSC) and FTIR, it is proposed that the lamella thickness of IPC15 is maximal and some ethylene segments exist in PP crystals of IPC15 as crystalline structure defects, which is responsible for this enhanced breakdown strength. The morphology results reveal that rubber microspheres are found to coexist with spherulites, which can promote the relative sliding among lamellas under external force and further results in the increase in the elongation at break.
Influence of Dry Milling on Phase Transformation of Sepiolite upon Alkali Activation: Implications for Textural, Catalytic and Sorptive Properties
Authors: Walczyk Anna , Karcz Robert , Kryściak-Czerwenka Joanna , Napruszewska Bogna D. , Duraczyńska Dorota , Michalik Alicja , Olejniczak Zbigniew , Tomczyk Anna , Klimek Agnieszka , Bahranowski Krzysztof , Serwicka Ewa M.
Published: September 2020

Activation of natural sepiolite by means of grinding in a planetary mill followed by wet NaOH activation was studied for the purpose of endowing the product with enhanced basicity for potential catalytic/sorptive applications. Synthesized solids were characterized with X-ray powder diffraction (XRD), N2 adsorption/desorption, scanning electron microscopy (SEM), energy dispersive (EDX), atomic absorption (AAS), Fourier-transform infrared (FTIR) and 29Si magic angle spinning nuclear magnetic resonance (MAS NMR) spectroscopies. Surface basicity was determined by titration with benzoic acid. Grinding changed the pathway of sepiolite phase transformation upon NaOH treatment. The as-received sepiolite evolved to Na-sepiolite (loughlinite) with a micropore system blocked by nanocrystalline Mg(OH)2, while ground samples yielded magnesium silicate hydrate phase (MSH), with well-developed microporous texture. In unmilled sepiolite desilication involved preferential leaching of Si from the center of the structural ribbons, while in ground samples additional loss of Si from ribbon-ribbon corner linkages was observed. In all cases treatment with NaOH led to enhancement of surface basicity. Synthesized materials were tested as catalysts in a base-catalyzed aldol self-condensation of acetone and oxidation of cyclohexanone to ε-caprolactone, as well as CO2 sorbents. Catalytic trends depended not only on samples’ basicity, but also on texture and phase composition of the catalysts. Grinding combined with alkali activation proved a simple and effective method for boosting CO2-sorption capacity of sepiolite to the level comparable to amine-functionalized, acid-activated sepiolite sorbents.
Influence of Sintering Process on Microstructure and Mechanical Properties of Ti(C,N)-Based Cermet
Authors: Ji Kaixun , Meng Yanxin , Wang Fuzeng , Li Yousheng
Published: September 2020

In this study, a Ti(C,N)-based cermet material was prepared through vacuum sintering. The research also investigates how holding time and maximum sintering temperature influence the material microstructure and mechanical properties. X-ray diffraction (XRD), energy dispersive spectroscopy (EDS) were used to analyze the composition of the cermet. The microstructure of the cermet was analyzed and examined using a scanning electron microscope (SEM). A Vickers hardness tester was used to test the mechanical properties of the materials. As indicated by testing results, the hardness of the material decreases as the temperature of sintering increases, and its fracture toughness increases gradually as holding time increases. Ti(C,N)-based cermet manifested the optimal mechanical properties when sintering was conducted under 1400 °C with 80 min of holding time. Moreover, the material microstructure is significantly affected by the sintering process. The grain size of Ti(C,N) cermets increases as the sintering temperature increases. The microstructure tends to be uniform and the complete core-rim structures are established as the holding time increases.
Experimental Research on the Influence of Different Curved Rigid Boundaries on Electric Spark Bubbles
Authors: Ma Chunlong , Shi Dongyan , Chen Yingyu , Cui Xiongwei , Wang Mengnan
Published: September 2020

It is well known that the bubble dynamics and load characteristics of cavitation bubbles depend to a great extent on their proximity to the boundary. The purpose of this study is to explore the relationship between the boundary curvature and bubble dynamics, as well as the load characteristics, and summarize the relevant change laws. This study takes three hemispheres of different curvatures and one flat board as its main research boundaries. The hemisphere was chosen as the curved surface boundary because the hemisphere represents the simplest type of curved surface boundary. This method allowed us to easily observe the experimental results and summarize the change laws of bubble dynamics and load characteristics. A high voltage electricity of 400 V was used to produce stable and repeatable electric spark bubbles in this experiment. Since the pulsation time of the bubbles is very short, we used a high-speed camera to acquire the necessary photographs. We also used a Hopkinson bar (HPB) to measure the bubble collapse load. Suppose that the dimensionless parameter of curvature is ζ and the dimensionless parameter of the explosion distance is γ. By summarizing the 44 groups of the experimental results under different combinations of ζ and γ, we found that the cavitation bubble dynamics and loading characteristics are affected by ζ. With an increase of ζ, the shockwave load and bubble collapse load will decrease. In addition, in terms of load characteristics, this study further verified the change trend of the shockwave load and bubble collapse load with γ. For the bubble shrink shape, this paper illustrates the relationship between the bubble’s shrink shape and its shrinkage speed. Four typical bubble shrink shapes are summarized. The effects of different ζ and γ values on the jet are preliminarily explored using the experimental results, and, by considering the experimental results, the developmental trends of the time of the bubble’s first pulsation period are discussed.
Numerical Mesoscale Analysis of Textile Reinforced Concrete
Authors: Fuchs Alexander , Curosu Iurie , Kaliske Michael
Published: September 2020

This contribution presents a framework for Numerical Material Testing (NMT) of textile reinforced concrete based on the mesomechanical analysis of a Representative Volume Element (RVE). Hence, the focus of this work is on the construction of a proper RVE representing the dominant mechanical characteristics of Textile Reinforced Concrete (TRC). For this purpose, the RVE geometry is derived from the periodic mesostructure. Furthermore, sufficient constitutive models for the individual composite constituents as well as their interfacial interactions are considered, accounting for the particular mechanical properties. The textile yarns are modeled as elastic transversal isotropic unidirectional layers. For the concrete matrix, an advanced gradient enhanced microplane model is utilized considering the complex plasticity and damage behavior at multiaxial loading conditions. The mechanical interactions of the constituents are modeled by an interface formulation considering debonding and friction as well as contact. These individual constitutive models are calibrated by corresponding experimental results. Finally, the damage mechanisms as well as the load bearing behavior of the constructed TRC-RVE are analyzed within an NMT procedure based on a first-order homogenization approach. Moreover, the effective constitutive characteristics of the composite at macroscale are derived. The numerical results are discussed and compared to experimental results.
Synthesis of Carbon Nanomaterials from Biomass Utilizing Ionic Liquids for Potential Application in Solar Energy Conversion and Storage
Authors: Mugadza Kudzai , Stark Annegret , Ndungu Patrick G. , Nyamori Vincent O.
Published: September 2020

Considering its availability, renewable character and abundance in nature, this review assesses the opportunity of the application of biomass as a precursor for the production of carbon-based nanostructured materials (CNMs). CNMs are exceptionally shaped nanomaterials that possess distinctive properties, with far-reaching applicability in a number of areas, including the fabrication of sustainable and efficient energy harnessing, conversion and storage devices. This review describes CNM synthesis, properties and modification, focusing on reports using biomass as starting material. Since biomass comprises 60–90% cellulose, the current review takes into account the properties of cellulose. Noting that highly crystalline cellulose poses a difficulty in dissolution, ionic liquids (ILs) are proposed as the solvent system to dissolve the cellulose-containing biomass in generating precursors for the synthesis of CNMs. Preliminary results with cellulose and sugarcane bagasse indicate that ILs can not only be used to make the biomass available in a liquefied form as required for the floating catalyst CVD technique but also to control the heteroatom content and composition in situ for the heteroatom doping of the materials.
Additive Manufacturing of Ti-48Al-2Cr-2Nb Alloy Using Gas Atomized and Mechanically Alloyed Plasma Spheroidized Powders
Authors: Polozov Igor , Kantyukov Artem , Goncharov Ivan , Razumov Nikolay , Silin Alexey , Popovich Vera , Zhu Jia-Ning , Popovich Anatoly
Published: September 2020

In this paper, laser powder-bed fusion (L-PBF) additive manufacturing (AM) with a high-temperature inductive platform preheating was used to fabricate intermetallic TiAl-alloy samples. The gas atomized (GA) and mechanically alloyed plasma spheroidized (MAPS) powders of the Ti-48Al-2Cr-2Nb (at. %) alloy were used as the feedstock material. The effects of L-PBF process parameters—platform preheating temperature—on the relative density, microstructure, phase composition, and mechanical properties of printed material were evaluated. Crack-free intermetallic samples with a high relative density of 99.9% were fabricated using 900 °C preheating temperature. Scanning electron microscopy and X-Ray diffraction analyses revealed a very fine microstructure consisting of lamellar α2/γ colonies, equiaxed γ grains, and retained β phase. Compressive tests showed superior properties of AM material as compared to the conventional TiAl-alloy. However, increased oxygen content was detected in MAPS powder compared to GA powder (~1.1 wt. % and ~0.1 wt. %, respectively), which resulted in lower compressive strength and strain, but higher microhardness compared to the samples produced from GA powder.
Mechanical Testing of Composite Steel and Reactive Powder Concrete Structural Element
Authors: Bujnak Jan , Michalek Peter , Bahleda Frantisek , Grzeszczyk Stefania , Matuszek-Chmurowska Aneta , Mordak Arkadiusz
Published: September 2020

Reactive powder concrete (RPC), typically with higher compressive strength, is particularly attractive to structural engineers to apply them in infrastructures for enhancing their resistance under severe environments and loads. The main objective of the initial study presented in the paper was to investigate the behavior of two types of these new cementitious materials differing in the nature of microfibers. The RPC mixes were reinforced with steel and then with basalt microfibers. To evaluate the structural performance of developed unconventional materials, properties were investigated experimentally and compared with the control normal concrete mix. Mechanical tests indicated that dispersing fine fibers for making RPC, a mean compressive strength of 198.3 MPa and flexural strength 52.6 MPa or 23.2 MPa, respectively, were developed after 28 days of standard curing at ambient temperatures. In composite structures consisting of steel girders and a concrete slab, it is necessary to prevent the relative slip at the steel and concrete interface using shear connectors. The very high RPC strength enabled a material saving, weight-reduced application of precast construction, and particularly effective joint to steel beams. The investigation of such shear connection efficiency, in the case of the higher strength concrete deck, using standard push-out test specimens was executed. Finite element numerical models were developed. The outputs of the studies are presented in the paper.
Developing an Analogue Residual Limb for Comparative DVC Analysis of Transtibial Prosthetic Socket Designs
Authors: Rankin Kathryn , Steer Joshua , Paton Joshua , Mavrogordato Mark , Marter Alexander , Worsley Peter , Browne Martin , Dickinson Alexander
Published: September 2020

Personalised prosthetic sockets are fabricated by expert clinicians in a skill- and experience-based process, with research providing tools to support evidence-based practice. We propose that digital volume correlation (DVC) may offer a deeper understanding of load transfer from prosthetic sockets into the residual limb, and tissue injury risk. This study’s aim was to develop a transtibial amputated limb analogue for volumetric strain estimation using DVC, evaluating its ability to distinguish between socket designs. A soft tissue analogue material was developed, comprising silicone elastomer and sand particles as fiducial markers for image correlation. The material was cast to form an analogue residual limb informed by an MRI scan of a person with transtibial amputation, for whom two polymer check sockets were produced by an expert prosthetist. The model was micro-CT scanned according to (i) an unloaded noise study protocol and (ii) a case study comparison between the two socket designs, loaded to represent two-legged stance. The scans were reconstructed to give 108 µm voxels. The DVC noise study indicated a 64 vx subvolume and 50% overlap, giving better than 0.32% strain sensitivity, and ~3.5 mm spatial resolution of strain. Strain fields induced by the loaded sockets indicated tensile, compressive and shear strain magnitudes in the order of 10%, with a high signal:noise ratio enabling distinction between the two socket designs. DVC may not be applicable for socket design in the clinical setting, but does offer critical 3D strain information from which existing in vitro and in silico tools can be compared and validated to support the design and manufacture of prosthetic sockets, and enhance the biomechanical understanding of the load transfer between the limb and the prosthesis.
The Usefulness of Pine Timber (Pinus sylvestris L.) for the Production of Structural Elements. Part I: Evaluation of the Quality of the Pine Timber in the Bending Test
Authors: Mirski Radosław , Dziurka Dorota , Chuda-Kowalska Monika , Wieruszewski Marek , Kawalerczyk Jakub , Trociński Adrian
Published: September 2020

The study assessed the quality of pine lumber by marking the modulus of elasticity in the horizontal system. The research material was a plank with the following dimensions: 137 mm wide × 39.50 mm thick × 3485 mm long. The pine wood was obtained by sawing timber in the form of logs with round cross sections and originating from the Forest Division Olesno (50°52′30″ N, 18°25′00″ E). Each long log was sawn to provide four logs of about 3.5 m, which were marked as butt-end logs (O), middle logs (S)—2 items, and top logs (W). The origin of the logs from the trunk (Pinus sylvestris L.) has a significant impact on the physical and mechanical properties of the wood from which they are made. Only butt-end logs (log type O) allows for the production of high-quality timber elements. The pine timber that was evaluated in this paper had a high density of about 570 kg/m3 and a high percentage of timber items were assigned to class C24 and higher (above 50%). The adopted horizontal model of evaluation of the modulus of elasticity gave similar results to those obtained in an evaluation according to the EN-408.
Finite Element Simulation of the Machining Process of Boiling Structures in a Novel Radial Heat Sink for High-Power LEDs
Authors: Xiang Jianhua , Liu Zeyu , Zhang Chunliang , Zhou Chao , Chen Conggui
Published: September 2020

A phase change heat sink has higher heat transfer efficiency compared to a traditional metal solid heat sink, and is thus more preferred for the heat dissipation of high-power light-emitting diodes (LEDs) with very high heat flux. The boiling structure at the evaporation surface is the biggest factor that affects heat sink resistance. It is necessary to investigate the plastic deformation law during the machining process of boiling structures. In this study, a novel phase change radial heat sink was developed for high-power LED heat dissipation. First, a working principle and a fabrication process for the heat sink were introduced. Subsequently, to achieve an excellent heat dissipation performance, the machining process of boiling structures was numerically simulated and investigated. To be specific, plastic deformation generated during the formation was analyzed, and key parameters related to the morphology of the boiling structures were discussed including feeding angles and machining depths. Moreover, the finite element (FE) simulation results were compared with those of experiments. Last but not least, the heat transfer performance of the fabricated heat sink was tested. Results showed that the developed heat sink was well suited for a high-power LED application.
Damage Propagation Analysis in the Single Lap Shear and Single Lap Shear-Riveted CFRP Joints by Acoustic Emission and Pattern Recognition Approach
Authors: Barile Claudia , Casavola Caterina , Pappalettera Giovanni , Vimalathithan Paramsamy Kannan
Published: September 2020

An innovative way of using the Acoustic Emission (AE) technique is introduced in this research work. The ratio of recorded acoustic energy and the counts recorded for each acoustic event were used for characterizing Carbon Fiber Reinforced Plastic (CFRP) laminates adhesively bonded with and without mechanical fasteners. The cumulative counts and cumulative energy of the recorded acoustic events were used for identifying the critical points of failure under loading of these hybrid joint specimens. The peak amplitude distribution was used for identifying the different damage modes such as delamination, matrix cracking and fiber breakage, albeit, ineffectively. The new parameter energy per count was introduced in this work, which can successfully identify the different damage modes under loading. To differentiate the damage modes using the energy per count, they were clustered using k-means++ pattern recognition technique. The method introduced in this work can estimate the damage modes of the CFRP specimens.
Relations between Generalized Bi-Periodic Fibonacci and Lucas Sequences
Authors: Choo Younseok
Published: September 2020

In this paper we consider a generalized bi-periodic Fibonacci {fn} and a generalized bi-periodic Lucas sequence {qn} which are respectively defined by f0=0, f1=1, fn=afn−1+cfn−2 (n is even) or fn=bfn−1+cfn−2 (n is odd), and q0=2d, q1=ad, qn=bqn−1+cqn−2 (n is even) or qn=afn−1+cqn−2 (n is odd). We obtain various relations between these two sequences.
Lifestyle, Lineage, and Geographical Origin Influence Temperature-Dependent Phenotypic Variation across Yeast Strains during Wine Fermentation
Authors: Deed Rebecca C. , Pilkington Lisa I.
Published: September 2020

Saccharomyces cerevisiae yeasts are a diverse group of single-celled eukaryotes with tremendous phenotypic variation in fermentation efficiency, particularly at different temperatures. Yeast can be categorized into subsets based on lifestyle (Clinical, Fermentation, Laboratory, and Wild), genetic lineage (Malaysian, Mosaic, North American, Sake, West African, and Wine), and geographical origin (Africa, Americas, Asia, Europe, and Oceania) to start to understand their ecology; however, little is known regarding the extent to which these groupings drive S. cerevisiae fermentative ability in grape juice at different fermentation temperatures. To investigate the response of yeast within the different subsets, we quantified fermentation performance in grape juice by measuring the lag time, maximal fermentation rate (Vmax), and fermentation finishing efficiency of 34 genetically diverse S. cerevisiae strains in grape juice at five environmentally and industrially relevant temperatures (10, 15, 20, 25, and 30 °C). Extensive multivariate analysis was applied to determine the effects of lifestyle, lineage, geographical origin, strain, and temperature on yeast fermentation phenotypes. We show that fermentation capability is inherent to S. cerevisiae and that all factors are important in shaping strain fermentative ability, with temperature having the greatest impact, and geographical origin playing a lesser role than lifestyle or genetic lineage.
Role of the Gut Microbiota in the Pathophysiology of Autism Spectrum Disorder: Clinical and Preclinical Evidence
Authors: Roussin Léa , Prince Naika , Perez-Pardo Paula , Kraneveld Aletta D. , Rabot Sylvie , Naudon Laurent
Published: September 2020

Autism spectrum disorder (ASD) is a neurodevelopmental disorder affecting 1 in 160 people in the world. Although there is a strong genetic heritability to ASD, it is now accepted that environmental factors can play a role in its onset. As the prevalence of gastrointestinal (GI) symptoms is four-times higher in ASD patients, the potential implication of the gut microbiota in this disorder is being increasingly studied. A disturbed microbiota composition has been demonstrated in ASD patients, accompanied by altered production of bacterial metabolites. Clinical studies as well as preclinical studies conducted in rodents have started to investigate the physiological functions that gut microbiota might disturb and thus underlie the pathophysiology of ASD. The first data support an involvement of the immune system and tryptophan metabolism, both in the gut and central nervous system. In addition, a few clinical studies and a larger number of preclinical studies found that modulation of the microbiota through antibiotic and probiotic treatments, or fecal microbiota transplantation, could improve behavior. Although the understanding of the role of the gut microbiota in the physiopathology of ASD is only in its early stages, the data gathered in this review highlight that this role should be taken in consideration.
Analytical Performances of the Panther Fusion System for the Detection of Respiratory Viruses in the French National Reference Centre of Lyon, France
Authors: Pichon Maxime , Valette Martine , Schuffenecker Isabelle , Billaud Geneviève , Lina Bruno
Published: September 2020

Respiratory infection are mainly caused by viral pathogens. During the 2017–2018 epidemic season, Panther Fusion® Respiratory kits (Influenza virus A&B (FluA&B), respiratory syncytial virus (RSV), adenovirus (ADV), metapneumovirus (MPV), rhinovirus (RV), parainfluenzae virus (PIV), were compared to the Respiratory MultiWells System r-gene. Respiratory clinical specimens were tested retrospectively (n = 268) and prospectively (n = 463). Analytical performances were determined (sensitivity –Sep-, specificity –Spe- and κ) considering concordances of ≥2 molecular testing specific to each viral target (discrepant results were verified at the National Reference Centres for Enteroviruses or Respiratory viruses, Lyon, France). After retrospective (and prospective) testing, Sep, Spe, and κ were 100% (97.7%), 100% (99%) and 100% (94%) for FluA: 100% (95.5%), 100% (99.3%) and 100% (94%) for FluB, and 100% (88.5%), 100% (98.7%) and 100% (89%) for RSV; 82.1% (41.7%), 100% (99.5%) and 86% (54%) for ADV; 94.7% (73.7%), 96.1% (98.0%) and 91% (65%) for MPV; 96.1% (94.6%), 90.2% (98.5%) and 86% (91%) for HRV; and 90% (72.7%), 100% (99.3%) and 91% (72%), respectively, for PIV. Analytical performances were above 85% for all viruses except for ADV, MPV and PIV, confirming the analytical performance of the Panther Fusion system, a high throughput system with reduced turn-around-time, when compared to non-automated systems.
Duration of Lactation and Maternal Risk of Metabolic Syndrome: A Systematic Review and Meta-Analysis
Authors: Tørris Christine , Bjørnnes Ann Kristin
Published: September 2020

Cardiovascular disease (CVD) is the leading cause of death of women across all ages, and targeting modifiable risk factors, such as those comprised in metabolic syndrome (MetS) (e.g., waist circumference, lipid profile, blood pressure, and blood glucose), is of great importance. An inverse association between lactation and CVD has been suggested, and lactation may decrease the risk of MetS. This systematic review and meta-analysis examined how lactation may affect the development and prevalence of MetS in women. A literature search was performed using Cinahl, Embase, Web of Science, and PubMed. A total of 1286 citations were identified, and finally, ten studies (two prospective and eight cross-sectional) were included. Seven studies (two prospective and five cross-sectional) revealed associations between lactation and MetS, suggesting that breastfeeding might prevent or improve metabolic health and have a protective role in MetS prevention. This protective role might be related to the duration of lactation; however, a lack of controlling for potential confounders, such as parity, might inflict the results. The pooled effect was non-conclusive. Additional research is required to further explore the duration of lactation and its potential role in improving or reversing MetS and its components.
Sodium and Potassium Intake in Residents of Retirement Homes
Authors: Rejec Boštjan , Golja Petra , Hlastan Ribič Cirila , Klemenc Matjaž
Published: September 2020

Excessive salt intake and its impact on health is a public health problem in many regions of the world. The currently estimated dietary intake of salt among free-living adults is well above the WHO recommendations. Over the years, the number of residents in retirement homes has increased. Besides this, the nutrition of elderly people may be affected by physiological changes that occur with aging. The question is whether residents of retirement homes receive a more balanced diet, or whether the trend of excessive salt consumption continues even among institutionalised elderly people. Salt and potassium intake were assessed by measuring sodium and potassium excretion over 24 h in urine collected from a sample of residents of three retirement homes in the Goriška region, Slovenia. The average salt intake was 8.3 (2.9) g/day, which was significantly higher (p < 0.001) in men than in women (10.1 (3.1) vs. 7.3 (2.2) g/day, respectively). The estimated total daily potassium intake was 2.6 (0.6) g/day in men and 2.0 (0.8) g/day in women (mean 2.2 (0.8) g/day). The ratio of sodium to potassium was 1.53 (0.48). The salt intake among residents of retirement homes in the Goriška region, especially in men, exceeds the WHO recommended daily intake of <5 g. The mean daily potassium intake was below the WHO recommendations of 3.5 g/day.
Secondary Sclerosing Cholangitis in Critically Ill Patients Alters the Gut–Liver Axis: A Case Control Study
Authors: Blesl Andreas , Jüngst Christoph , Lammert Frank , Fauler Günter , Rainer Florian , Leber Bettina , Feldbacher Nicole , Stromberger Silvia , Wildburger Renate , Spindelböck Walter , Fickert Peter , Horvath Angela , Stadlbauer Vanessa
Published: September 2020

Secondary sclerosing cholangitis in critically ill patients (SC-CIP) occurs after long-term intensive care treatment. This study aimed to assess the gut–liver axis in SC-CIP. Stool microbiome composition, gut permeability, bacterial translocation and serum bile acid profiles of 18 SC-CIP patients compared to 11 patients after critical illness without liver disease (CIP controls), 21 patients with cirrhosis and 21 healthy controls were studied. 16S rDNA was isolated from stool and sequenced using the Illumina technique. Diamine oxidase, zonulin, soluble CD14 (sCD14) and lipopolysaccharide binding protein were measured in serum and calprotectin in stool. Serum bile acids were analyzed by high-performance liquid chromatography-mass spectrometry (HPLC-MS). Reduced microbiome alpha diversity and altered beta diversity were seen in SC-CIP, CIP controls and cirrhosis compared to healthy controls. SC-CIP patients showed a shift towards pathogenic taxa and an oralization. SC-CIP, CIP controls and cirrhotic patients presented with impaired gut permeability, and biomarkers of bacterial translocation were increased in SC-CIP and cirrhosis. Total serum bile acids were elevated in SC-CIP and cirrhosis and the bile acid profile was altered in SC-CIP, CIP controls and cirrhosis. In conclusions, observed alterations of the gut–liver axis in SC-CIP cannot solely be attributed to liver disease, but may also be secondary to long-term intensive care treatment.
Saponin Micelles Lead to High Mucosal Permeation and In Vivo Efficacy of Solubilized Budesonide
Authors: Nakowitsch Sabine , Koller Christiane , Seifert Jan-Marcus , König-Schuster Marielle , Unger-Manhart Nicole , Siegl Cornelia , Kirchoff Norman , Foglar Elisabeth , Graf Christine , Morokutti-Kurz Martina , Neurath Marianne , Sladek Svenja , Knecht Christian , Sipos Wolfgang , Prieschl-Grassauer Eva , Grassauer Andreas
Published: September 2020

Due to fast nasal mucociliary clearance, only the dissolved drug content can effectively permeate the mucosa and be pharmaceutically active after intranasal application of suspensions. Therefore, the aim of this study was to increase the budesonide concentration in solution of a nasal spray formulation. Budesonide, a highly water-insoluble corticosteroid, was successfully solubilized using a micellar formulation comprising escin, propylene glycol and dexpanthenol in an aqueous buffered environment (“Budesolv”). A formulation based on this micellar system was well-tolerated in the nasal cavity as shown in a good laboratory practice (GLP) local tolerance study in rabbits. Ex vivo permeation studies into porcine nasal mucosa revealed a faster and more efficient absorption. Budesolv with 300 µg/mL solubilized budesonide resulted in a budesonide concentration of 42 µg/g tissue after only 15 min incubation. In comparison, incubation with the marketed product Rhinocort® aqua 64 (1.28 mg/mL budesonide as suspension) led to 15 µg/g tissue. The in vivo tumor-necrosis-factor (TNF)-α secretion in an acute lung inflammation mouse model was significantly reduced (p < 0.001) following a prophylactic treatment with Budesolv compared to Rhinocort® aqua 64. Successful treatment 15 min after the challenge was only possible with Budesolv (40% reduction of TNF-α, p = 0.0012) suggesting a faster onset of action. The data reveal that solubilization based on saponin micelles presents an opportunity for the development of products containing hardly soluble substances that result in a faster onset and a better topical treatment effect.
Thermo-Responsive Shape Memory Effect and Conversion of Porous Structure in a Polyvinyl Chloride Foam
Authors: Wang Tao Xi , Chang Lu Lu , Geng Yun Hui , Shen Xing
Published: September 2020

In this paper, a thermo-responsive shape memory effect in a polyvinyl chloride thermoset foam is characterized. Excellent shape recovery performance is observed in foam samples programmed both at room temperature and above their transition temperature. The conversion of porous structures in the foam from closed-cell to open-cell after a shape memory effect cycle is revealed via a series of specially designed oil-dripping experiments and optical images of the micro pores. Programming the strain higher than 20% results in an apparent increase in open-cell level, whereas programming temperatures have almost no influence.
Effect of Exposure Temperature on the Crashworthiness of Carbon/Epoxy Composite Rectangular Tubes Under Quasi-Static Compression
Authors: Sebaey Tamer A.
Published: September 2020

The exposure of polymeric composites to thermal loading is a ubiquitous problem that leads to the degradation of mechanical properties, reducing the service life of an engineered structure, and potentially premature, catastrophic modes of failure. In the current paper, an experimental study is presented in order to investigate the effect of thermal exposure on the crushing performance of carbon fiber-reinforced plastic (CFRP) composite tubes. Specimens of rectangular tubes are subjected to thermal exposure at 90, 120, 150 and 180 °C before being crushed under quasi-static loading. The results showed a reduction in the peak load by increasing the aging temperature up to °C, which is followed by an increase in the peak load at 150 °C, due to post-curing. For the energy absorbed and the specific energy, a sharp reduction is recorded (up to 70% reduction) due to thermal aging. These results showed that the effect of thermal exposure on crashworthiness needs more attention during composites’ design, especially for transportation applications.