bluuebunny/crossref_metadata_2025 huggingface dataset:
Start Me Up: How Can Surrounding Gangliosides Affect Sodium-Potassium ATPase Activity and Steer towards Pathological Ion Imbalance in Neurons?
Authors: Puljko Borna , Stojanović Mario , Ilic Katarina , Kalanj-Bognar Svjetlana , Mlinac-Jerkovic Kristina
Published: June 2022

Gangliosides, amphiphilic glycosphingolipids, tend to associate laterally with other membrane constituents and undergo extensive interactions with membrane proteins in cis or trans configurations. Studies of human diseases resulting from mutations in the ganglioside biosynthesis pathway and research on transgenic mice with the same mutations implicate gangliosides in the pathogenesis of epilepsy. Gangliosides are reported to affect the activity of the Na+/K+-ATPase, the ubiquitously expressed plasma membrane pump responsible for the stabilization of the resting membrane potential by hyperpolarization, firing up the action potential and ion homeostasis. Impaired Na+/K+-ATPase activity has also been hypothesized to cause seizures by several mechanisms. In this review we present different epileptic phenotypes that are caused by impaired activity of Na+/K+-ATPase or changed membrane ganglioside composition. We further discuss how gangliosides may influence Na+/K+-ATPase activity by acting as lipid sorting machinery providing the optimal stage for Na+/K+-ATPase function. By establishing a distinct lipid environment, together with other membrane lipids, gangliosides possibly modulate Na+/K+-ATPase activity and aid in “starting up” and “turning off” this vital pump. Therefore, structural changes of neuronal membranes caused by altered ganglioside composition can be a contributing factor leading to aberrant Na+/K+-ATPase activity and ion imbalance priming neurons for pathological firing.
The Impact of Metabolic Syndrome and Obesity on the Evolution of Diastolic Dysfunction in Apparently Healthy Patients Suffering from Post-COVID-19 Syndrome
Authors: Tudoran Cristina , Tudoran Mariana , Cut Talida Georgiana , Lazureanu Voichita Elena , Bende Felix , Fofiu Renata , Enache Alexandra , Pescariu Silvius Alexandru , Novacescu Dorin
Published: June 2022

(1) Background: Coronavirus disease 2019 (COVID-19) has a worse prognosis in individuals with obesity and metabolic syndrome (MS), who often develop cardiovascular complications that last throughout recovery. (2) Methods: This study aimed to analyze the evolution of diastolic dysfunction (DD), assessed by transthoracic echocardiography (TTE), in 203 individuals with and without obesity and/or MS diagnosed with post-COVID-19 syndrome. (3) Results: DD was frequently diagnosed in patients with MS and obesity, but also in those without obesity (62.71% and 56.6%, respectively), in comparison to 21.97% of subjects without MS (p ˂ 0.001). Almost half of the patients with obesity and MS had more severe DD (types 2 and 3). As for evolution, the prevalence and severity of DD, particularly types 1 and 2, decreased gradually, in parallel with the improvement of symptoms, progress being more evident in subjects without MS. DD of type 3 did not show a significant reduction (p = 0.47), suggesting irreversible myocardial damages. Multivariate regression analysis indicated that the number of MS factors, the severity of initial pulmonary injury, and protein C levels could explain DD evolution. (4) Conclusions: DD was commonly diagnosed in individuals with post-COVID-19 syndrome, particularly in those with MS and obesity. After 6 months, DD evolution, excepting that of type 3, showed a significant improvement, mostly in patients without MS.
Cochlear Implantation Outcomes in Patients with Auditory Neuropathy Spectrum Disorder of Genetic and Non-Genetic Etiologies: A Multicenter Study
Authors: Lin Pei-Hsuan , Wu Hung-Pin , Wu Che-Ming , Chiang Yu-Ting , Hsu Jacob Shujui , Tsai Cheng-Yu , Wang Han , Tseng Li-Hui , Chen Pey-Yu , Yang Ting-Hua , Hsu Chuan-Jen , Chen Pei-Lung , Wu Chen-Chi , Liu Tien-Chen
Published: June 2022

With diverse etiologies and clinical features, the management of pediatric auditory neuropathy spectrum disorder (ANSD) is often challenging, and the outcomes of cochlear implants (CIs) are variable. This study aimed to investigate CI outcomes in pediatric patients with ANSD of different etiologies. Thirty-six children with ANSD who underwent cochlear implantation between 2001 and 2021 were included. Comprehensive etiological analyses were conducted, including a history review, next-generation sequencing-based genetic examinations, and imaging studies using high-resolution computed tomography and magnetic resonance imaging. Serial behavioral and speech audiometry were performed before and after surgery, and the outcomes with CI were evaluated using the Categories of Auditory Performance (CAP) and Speech Intelligibility Rating (SIR) scores. By etiology, 18, 1, 1, and 10 patients had OTOF-related, WFS1-related, OPA1-related, and cochlear nerve deficiency (CND)-related ANSD, respectively. Six patients had no definite etiology. The average CI-aided behavioral threshold was 28.3 ± 7.8 dBHL, and those with CND-related ANSD were significantly worse than OTOF-related ANSD. The patients’ median CAP and SIR scores were 6 and 4, respectively. Favorable CI outcomes were observed in patients with certain etiologies of ANSD, particularly those with OTOF (CAP/SIR scores 5–7/2–5), WFS1 (CAP/SIR score 6/5), and OPA1 variants (CAP/SIR score 7/5). Patients with CND had suboptimal CI outcomes (CAP/SIR scores 2–6/1–3). Identifying the etiologies in ANSD patients is crucial before surgery and can aid in predicting prognoses.
Fluorescent Biosensors for the Detection of Viruses Using Graphene and Two-Dimensional Carbon Nanomaterials
Authors: Salama Ahmed M. , Yasin Ghulam , Zourob Mohammed , Lu Jun
Published: June 2022

Two-dimensional carbon nanomaterials have been commonly employed in the field of biosensors to improve their sensitivity/limits of detection and shorten the analysis time. These nanomaterials act as efficient transducers because of their unique characteristics, such as high surface area and optical, electrical, and magnetic properties, which in turn have been exploited to create simple, quick, and low-cost biosensing platforms. In this review, graphene and two-dimensional carbon material-based fluorescent biosensors are covered between 2010 and 2021, for the detection of different human viruses. This review specifically focuses on the new developments in graphene and two-dimensional carbon nanomaterials for fluorescent biosensing based on the Förster resonance energy transfer (FRET) mechanism. The high-efficiency quenching capability of graphene via the FRET mechanism enhances the fluorescent-based biosensors. The review provides a comprehensive reference for the different types of carbon nanomaterials employed for the detection of viruses such as Rotavirus, Ebola virus, Influenza virus H3N2, HIV, Hepatitis C virus (HCV), and Hepatitis B virus (HBV). This review covers the various multiplexing detection technologies as a new direction in the development of biosensing platforms for virus detection. At the end of the review, the different challenges in the use of fluorescent biosensors, as well as some insights into how to overcome them, are highlighted.
Photoacoustic/Ultrasound Endoscopic Imaging Reconstruction Algorithm Based on the Approximate Gaussian Acoustic Field
Authors: Wang Yongjun , Yuan Chuqi , Jiang Jinsheng , Peng Kuan , Wang Bo
Published: June 2022

This paper aims to propose a new photoacoustic/ultrasound endoscopic imaging reconstruction algorithm based on the approximate Gaussian acoustic field which significantly improves the resolution and signal-to-noise ratio (SNR) of the out-of-focus region. We demonstrated the method by numerical calculations and investigated the applicability of the algorithm in a chicken breast phantom. The validation was finally performed by the rabbit rectal endoscopy experiment. Simulation results show that the lateral resolution of the target point in the out-of-focus region can be well optimized with this new algorithm. Phantom experimental results show that the lateral resolution of the indocyanine green (ICG) tube in the photoacoustic image is reduced from 3.975 mm to 1.857 mm by using our new algorithm, which is a 52.3% improvement. Ultrasound images also show a significant improvement in lateral resolution. The results of the rabbit rectal endoscopy experiment prove that the algorithm we proposed is capable of providing higher-quality photoacoustic/ultrasound images. In conclusion, the algorithm enables fast acoustic resolution photoacoustic/ ultrasonic dynamic focusing and effectively improves the imaging quality of the system, which has significant guidance for the design of acoustic resolution photoacoustic/ultrasound endoscopy systems.
Development of GO/Co/Chitosan-Based Nano-Biosensor for Real-Time Detection of D-Glucose
Authors: Kim Dong Sup , Yang Xiaoguang , Lee Ja Hyun , Yoo Hah Young , Park Chulhwan , Kim Seung Wook , Lee Jinyoung
Published: June 2022

Electrochemical nano-biosensor systems are popular in the industrial field, along with evaluations of medical, agricultural, environmental and sports analysis, because they can simultaneously perform qualitative and quantitative analyses with high sensitivity. However, real-time detection using an electrochemical nano-biosensor is greatly affected by the surrounding environment with the performance of the electron transport materials. Therefore, many researchers are trying to find good factors for real-time detection. In this work, it was found that a composite composed of graphite oxide/cobalt/chitosan had strong stability and electron transfer capability and was applied to a bioelectrochemical nano-biosensor with high sensitivity and stability. As a mediator-modified electrode, the GO/Co/chitosan composite was electrically deposited onto an Au film electrode by covalent boding, while glucose oxidase as a receptor was immobilized on the end of the GO/Co/chitosan composite. It was confirmed that the electron transfer ability of the GO/Co/chitosan composite was excellent, as shown with power density analysis. In addition, the real-time detection of D-glucose could be successfully performed by the developed nano-biosensor with a high range of detected concentrations from 1.0 to 15.0 mM. Furthermore, the slope value composed of the current, per the concentration of D-glucose as a detection response, was significantly maintained even after 14 days.
Mental Stress Assessment Using Ultra Short Term HRV Analysis Based on Non-Linear Method
Authors: Lee Seungjae , Hwang Ho Bin , Park Seongryul , Kim Sanghag , Ha Jung Hee , Jang Yoojin , Hwang Sejin , Park Hoon-Ki , Lee Jongshill , Kim In Young
Published: June 2022

Mental stress is on the rise as one of the major health problems in modern society. It is important to detect and manage mental stress to prevent various diseases caused by stress and to maintain a healthy life. The purpose of this paper is to present new heart rate variability (HRV) features based on empirical mode decomposition and to detect acute mental stress through short-term HRV (5 min) and ultra-short-term HRV (under 5 min) analysis. HRV signals were acquired from 74 young police officers using acute stressors, including the Trier Social Stress Test and horror movie viewing, and a total of 26 features, including the proposed IMF energy features and general HRV features, were extracted. A support vector machine (SVM) classification model is used to classify the stress and non-stress states through leave-one-subject-out cross-validation. The classification accuracies of short-term HRV and ultra-short-term HRV analysis are 86.5% and 90.5%, respectively. In the results of ultra-short-term HRV analysis using various time lengths, we suggest the optimal duration to detect mental stress, which can be applied to wearable devices or healthcare systems.
Stereotactic Surgery for Treating Intractable Tourette Syndrome: A Single-Center Pilot Study
Authors: Wang Xin , Qu Liang , Ge Shunnan , Li Nan , Wang Jing , Qiu Chun , Kou Huijuan , Li Jiaming , Jing Jiangpeng , Su Mingming , Zheng Zhaohui , Li Yang , Qu Yan , Wang Xuelian
Published: June 2022

To evaluate the potential effect of radiofrequency ablation and deep brain stimulation in patients with treatment-refractory Tourette syndrome (TS), this study enrolled thirteen patients with TS who were admitted to our hospital between August 2002 and September 2018. Four patients received a single- or multi-target radiofrequency ablation after local, potentiated, or general anesthesia; eight patients underwent deep brain stimulation (DBS) surgery; and one patient underwent both ablation and DBS surgery. The severity of tics and obsessive compulsive disorder symptoms and the quality of life were evaluated using the Yale Global Tic Severity Scale (YGTSS), Yale–Brown Obsessive Compulsive Scale (YBOCS), and Gilles de la Tourette Syndrome Quality of Life scale (GTS-QOL), respectively, before surgery, one month after surgery, and at the final follow-up after surgery, which was conducted in December 2018. A paired-sample t test and a multiple linear regression analysis were performed to analyze the data. All patients underwent the operation successfully without any severe complications. Overall, the YGTSS total scores at one month post-surgery (44.1 ± 22.3) and at the final visit (35.1 ± 23.7) were significantly decreased compared with those at baseline (75.1 ± 6.2; both p < 0.05). Additionally, the YBOCS scores at one month post-surgery (16.5 ± 10.1) and at the final visit (12.0 ± 9.5) were significantly decreased compared with those at baseline (22.5 ± 13.1; both p < 0.05). Furthermore, the GTS-QOL scores at one month post-surgery (44.0 ± 12.8) and at the final visit (31.0 ± 17.8) were significantly decreased compared with those at baseline (58.4 ± 14.2; both p < 0.05). Results from a multiple linear regression analysis revealed that the improvement in the YGTSS total score was independently associated with the improvement in the GTS-QOL score at one month post-surgery (standardized β = 0.716, p = 0.023) and at the final visit (standardized β = 1.064, p = 0.000). Conversely, changes in YBOCS scores did not correlate with changes in GTS-QOL scores (p > 0.05). Our results demonstrate that tics, psychiatric symptoms, and the quality of life in patients with intractable TS may be relieved by stereotactic ablation surgery and deep brain stimulation. Furthermore, it appears that the improvement in tics contributes more to the post-operative quality of life of patients than does the improvement in obsessive compulsive symptoms.
Effect of Posidonia oceanica Fibers Addition on the Thermal and Acoustic Properties of Cement Paste
Authors: Benjeddou Omrane , Jedidi Malek , Khadimallah Mohamed Amine , Ravindran Gobinath , Sridhar Jayaprakash
Published: June 2022

The present work focused on the experimental study of the mechanical, thermal and acoustic properties of cement composite reinforced using Posidonia oceanica (PO) fibers. For this purpose, parallelepipedic specimens of dimensions 270 mm × 270 mm × 40 mm and cubic specimens of dimensions 150 mm × 150 mm × 150 mm were prepared with a water-to-cement ratio of 0.50 by varying the volume of fibers (Vf) from 0% to 20%. Properties such as compressive strength, thermal conductivity, thermal diffusivity, standardized level difference and sound transmission class were examined. The compressive strength of the specimens was determined using the rebound hammer test, while the thermal measurements were performed with the steady-state box method. The results showed that the addition of PO fibers improved the compressive strength of the mixtures and produced a maximum value of 33.60 MPa for a 10% volume of fiber content. Thermal conductivity and thermal diffusivity decreased significantly with the addition of fibers for all the mixtures. The experimental investigation also showed that the sound transmission class of PO-fiber-reinforced cementitious composites decreased as the fiber volume increased due to an increase in air voids in the mixtures.
Hydrogen Storages Based on Graphene Nano-Flakes: Density Functional Theory Approach
Authors: Tachikawa Hiroto
Published: June 2022

Carbon materials such as graphene, carbon nanotubes, fullerene, and graphene nanoflakes (GNFs) are used for hydrogen storage. The doping of alkali metals to these materials generally increases the accumulation density of molecular hydrogen (H2). However, the reason why the doping enhances the ability of the H2 storage of GNF is not clearly known, although there are some explanations. In addition, the information on the storage capacity of GNF is ambiguous. In the present review article, we introduce our recent theoretical studies on the interaction of GNF with H2 molecules carried out to elucidate the mechanism of hydrogen storage in alkali-doped GNFs. As alkali metals, lithium (Li), sodium (Na), and potassium (K) were examined, and the abilities of hydrogen storage were discussed. Next, the mechanism of Li-diffusion on GNF, which plays a crucial role in Li-battery, was presented. There are several unanswered questions. In particular, does lithium diffuse randomly on GNF? Or is there a specific diffusion path? We present our study, which elucidates the factors governing lithium diffusion on GNF. If the dominant factor is known, it is possible to arbitrarily control the diffusion path of lithium. This will lead to the development of highly functional battery materials. Finally, the molecular design of H adsorption–desorption reversible storage devices based on GNF will be introduced. Elucidating the mechanism of hydrogen storage, Li-diffusion on GNF, and molecular design of storage device is important in understanding the current molecular devices and provide a deeper insight into materials chemistry.
Cancer-Associated Fibroblasts Promote Tumor Aggressiveness in Head and Neck Cancer through Chemokine Ligand 11 and C-C Motif Chemokine Receptor 3 Signaling Circuit
Authors: Huang Wen-Yen , Lin Yaoh-Shiang , Lin Yu-Chun , Nieh Shin , Chang Yi-Ming , Lee Tsai-Yu , Chen Su-Feng , Yang Kuender D.
Published: June 2022

The tumor microenvironment (TME) plays a crucial role in tumor progression. One of its key stromal components, cancer-associated fibroblasts (CAFs), may crosstalk with cancer cells by secreting certain cytokines or chemokines. However, which important mediator(s) are released by CAFs, and the underlying molecular mechanism, remain largely unknown. In the present study, we isolated patient-derived CAFs and normal fibroblasts (NFs). Using microarray analysis, we detected chemokine ligand 11 (CCL11) overexpression in CAFs compared to NFs. CCL11 administration promoted the migration and invasion of head and neck cancer (HNC) cells with enhanced cancer stem cell-like properties and induction of epithelial-to-mesenchymal transition. Furthermore, neutralization of CCL11 activity reversed the aggressive phenotype of CAF-induced cancer cells. Confocal microscopy showed colocalization of CCL11 and CC chemokine receptor 3 (CCR3) on HNC cells. Moreover, immunohistochemical analysis of clinical samples from 104 patients with HNC showed that expression of CCL11 and CCR3 were significantly correlated with poor overall survival (p = 0.003 and 0.044, respectively). Collectively, CCL11 expressed on CAFs promotes HNC invasiveness, and neutralization of CCL11 reverses this effect. We propose that the CCL11/CCR3 signaling circuit is a potential target for optimizing therapeutic strategies against HNC.
p53 Isoforms as Cancer Biomarkers and Therapeutic Targets
Authors: Zhao Liuqun , Sanyal Suparna
Published: June 2022

This review aims to summarize the implications of the major isoforms of the tumor suppressor protein p53 in aggressive cancer development. The current knowledge of p53 isoforms, their involvement in cell-signaling pathways, and their interactions with other cellular proteins or factors suggests the existence of an intricate molecular network that regulates their oncogenic function. Moreover, existing literature about the involvement of the p53 isoforms in various cancers leads to the proposition of therapeutic solutions by altering the cellular levels of the p53 isoforms. This review thus summarizes how the major p53 isoforms Δ40p53α/β/γ, Δ133p53α/β/γ, and Δ160p53α/β/γ might have clinical relevance in the diagnosis and effective treatments of cancer.
Ovarian Adnexal Reporting Data System (O-RADS) for Classifying Adnexal Masses: A Systematic Review and Meta-Analysis
Authors: Vara Julio , Manzour Nabil , Chacón Enrique , López-Picazo Ana , Linares Marta , Pascual Maria Ángela , Guerriero Stefano , Alcázar Juan Luis
Published: June 2022

In this systematic review and meta-analysis, we aimed to assess the pooled diagnostic performance of the so-called Ovarian Adnexal Report Data System (O-RADS) for classifying adnexal masses using transvaginal ultrasound, a classification system that was introduced in 2020. We performed a search for studies reporting the use of the O-RADS system for classifying adnexal masses from January 2020 to April 2022 in several databases (Medline (PubMed), Google Scholar, Scopus, Cochrane, and Web of Science). We selected prospective and retrospective cohort studies using the O-RADS system for classifying adnexal masses with histologic diagnosis or conservative management demonstrating spontaneous resolution or persistence in cases of benign appearing masses after follow-up scan as the reference standard. We excluded studies not related to the topic under review, studies not addressing O-RADS classification, studies addressing MRI O-RADS classification, letters to the editor, commentaries, narrative reviews, consensus documents, and studies where data were not available for constructing a 2 × 2 table. The pooled sensitivity, specificity, positive and negative likelihood ratios, and diagnostic odds ratio (DOR) were calculated. The quality of the studies was evaluated using QUADAS-2. A total of 502 citations were identified. Ultimately, 11 studies comprising 4634 masses were included. The mean prevalence of ovarian malignancy was 32%. The risk of bias was high in eight studies for the “patient selection” domain. The risk of bias was low for the “index test” and “reference test” domains for all studies. Overall, the pooled estimated sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, and DOR of the O-RADS system for classifying adnexal masses were 97% (95% confidence interval (CI) = 94%–98%), 77% (95% CI = 68%–84%), 4.2 (95% CI = 2.9–6.0), 0.04 (95% CI = 0.03–0.07), and 96 (95% CI = 50–185), respectively. Heterogeneity was moderate for sensitivity and high for specificity. In conclusion, the O-RADS system has good sensitivity and moderate specificity for classifying adnexal masses.
In Situ Growth of NiSe2-MoSe2 Heterostructures on Graphene Nanosheets as High-Performance Electrocatalyst for Hydrogen Evolution Reaction
Authors: Dai Tianjun , Zhou Zhangyu , Xiao Han , Luo Yingchun , Xu Yongchi , Wang Xinqiang
Published: June 2022

Developing highly efficient and stable electrocatalysts for hydrogen evolution reaction (HER) is regarded as a crucial way to reduce energy loss in water splitting. Herein, NiSe2/MoSe2 heterostructures grown on graphene nanosheets (NiSe2-MoSe2 HTs/G) have been in situ synthesized by a simple hydrothermal reaction. As an electrocatalyst for HER, NiSe2-MoSe2 HTs/G delivers superior performance with a low Tafel slope of 65 mV dec−1, a small overpotential of 144 mV at 10 mA cm−2, and long-term stability up to 24 h. The superior performance for HER can be mainly ascribed to the synergistic effects of NiSe2-MoSe2 heterostructures, which can facilitate the rapid electron transfer from the electrode to the exposed MoSe2 edges to take part in the HER reaction, thus boosting the HER kinetics. Moreover, the graphene matrix with high conductivity can not only improve the overall conductivity of the composite but also greatly increase the exposed active sites, therefore further promoting the HER performance. This study provides a simple route for fabricating bimetallic selenides-based heterostructures on graphene as an efficient and stable electrocatalyst for HER.
High-Performance A-Site Deficient Perovskite Electrocatalyst for Rechargeable Zn–Air Battery
Authors: Wang Chengcheng , Hou Bingxue , Wang Xintao , Yu Zhan , Luo Dawei , Gholizadeh Mortaza , Fan Xincan
Published: June 2022

Zinc–air batteries are one of the most excellent of the next generation energy devices. However, their application is greatly hampered by the slow kinetics of oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) of air electrode. It is of great importance to develop good oxygen electrocatalysts with long durability as well as low cost. Here, A-site deficient (SmSr)0.95Co0.9Pt0.1O3 perovskites have been studied as potential OER electrocatalysts prepared by EDTA–citrate acid complexing method. OER electrocatalytic performance of (SmSr)0.95Co0.9Pt0.1O3 was also evaluated. (SmSr)0.95Co0.9Pt0.1O3 electrocatalysts exhibited good OER activities in 0.1 M KOH with onset potential and Tafel slope of 1.50 V and 87 mV dec−1, similar to that of Ba0.5Sr0.5Co0.8Fe0.2O3 (BSCF-5582). Assembled rechargeable Zn–air batteries exhibited good discharge potential and charge potential with high stability, respectively. Overall, all results illustrated that (SmSr)0.95Co0.9Pt0.1O3 is an excellent OER electrocatalyst for zinc–air batteries. Additionally, this work opens a good way to synthesize highly efficient electrocatalysts from A-site deficient perovskites.
Homogeneous Group IVB Catalysts of New Generations for Synthesis of Ethylene-Propylene-Diene Rubbers: A Mini-Review
Authors: Bravaya Natalia M. , Faingol’d Evgeny E. , Sanginov Evgeny A. , Badamshina Elmira R.
Published: June 2022

Ethylene-propylene-diene rubbers (EPDM) are one of the most important polyolefin materials widely commercialized and used in various industries in recent years. The production of EPDM is based solely on catalytic coordination polymerization processes. The development of new catalysts and processes for the synthesis of EPDM has expanded the range of products and their manufacturing in terms of energy efficiency, processability, and environmental safety. This mini-review mainly analyzes patented data on the synthesis of EPDM on new-generation single-site catalytic systems based on Group IVB complexes including the systems commercialized by major manufacturers of EPDM. The advantages of these systems are evident in comparison with conventional vanadium systems introduced into production in the 1960s and used to date in the industrial synthesis of EPDM.
Gold-Based Catalysts for Complete Formaldehyde Oxidation: Insights into the Role of Support Composition
Authors: Ilieva Lyuba , Dimitrov Dimitar , Kolentsova Elitsa , Venezia Anna Maria , Karashanova Daniela , Avdeev Georgi , Petrova Petya , State Razvan , Tabakova Tatyana
Published: June 2022

Formaldehyde (HCHO) is recognized as one of the most emitted indoor air pollutants with high detrimental effect on human health. Significant research efforts are focused on HCHO removal to meet emission regulations in an effective and economically profitable way. For over three decades, the unique electronic properties and catalytic abilities of nano-gold catalysts continue to be an attractive research area for the catalytic community. Recently, we reported that mechanochemical mixing is a relevant approach to the preparation of Co-Ce mixed oxides with high activity in complete benzene oxidation. A trend of higher surface defectiveness, in particular, oxygen vacancies, caused by close interaction between cobalt oxide and cerium oxide phases, was observed for a mixed oxide composition of 70 wt.% Co3O4 and 30 wt.% CeO2. These results directed further improvement by promotion with gold and optimization of mixed oxide composition, aiming for the development of an efficient catalyst for room temperature HCHO abatement. Support modification with potassium was studied; however, the K addition caused less enhancement of HCHO oxidation activity than expected. This motivated the preparation of new carrier material. In addition to Co3O4-CeO2 mixed metal oxides with preset ratio, γ-Al2O3 intentionally containing 33% boehmite and shortly named Al2O3-b was used for synthesis. Analysis of the role of support composition in HCHO oxidation was based on the characterization of nano-gold catalysts by textural measurements, XRD, HRTEM, XPS, and TPR techniques. Gold supported on mechanochemically treated Co3O4-CeO2-Al2O3-b (50 wt.% Al2O3-b) exhibited superior activity owing to high Ce3+ and Co3+ surface amounts and the most abundant oxygen containing species with enhanced mobility. This catalyst achieved oxidation to CO2 and H2O by 95% HCHO conversion at room temperature and 100% at 40 °C, thus implying the potential of this composition in developing efficient catalytic materials for indoor air purification.
Aging-Associated Changes in Cognition, Expression and Epigenetic Regulation of Chondroitin 6-Sulfotransferase Chst3
Authors: Baidoe-Ansah David , Sakib Sadman , Jia Shaobo , Mirzapourdelavar Hadi , Strackeljan Luisa , Fischer Andre , Aleshin Stepan , Kaushik Rahul , Dityatev Alexander
Published: June 2022

Understanding changes in the expression of genes involved in regulating various components of the neural extracellular matrix (ECM) during aging can provide an insight into aging-associated decline in synaptic and cognitive functions. Hence, in this study, we compared the expression levels of ECM-related genes in the hippocampus of young, aged and very aged mice. ECM gene expression was downregulated, despite the accumulation of ECM proteoglycans during aging. The most robustly downregulated gene was carbohydrate sulfotransferase 3 (Chst3), the enzyme responsible for the chondroitin 6-sulfation (C6S) of proteoglycans. Further analysis of epigenetic mechanisms revealed a decrease in H3K4me3, three methyl groups at the lysine 4 on the histone H3 proteins, associated with the promoter region of the Chst3 gene, resulting in the downregulation of Chst3 expression in non-neuronal cells. Cluster analysis revealed that the expression of lecticans—substrates of CHST3—is tightly co-regulated with this enzyme. These changes in ECM-related genes were accompanied by an age-confounded decline in cognitive performance. Despite the co-directional impairment in cognitive function and average Chst3 expression in the studied age groups, at the individual level we found a negative correlation between mRNA levels of Chst3 and cognitive performance within the very aged group. An analysis of correlations between the expression of ECM-related genes and cognitive performance in novel object versus novel location recognition tasks revealed an apparent trade-off in the positive gene effects in one task at the expense of another. Further analysis revealed that, despite the reduction in the Chst3 mRNA, the expression of CHST3 protein is increased in glial cells but not in neurons, which, however, does not lead to changes in the absolute level of C6S and even results in the decrease in C6S in perineuronal, perisynaptic and periaxonal ECM relative to the elevated expression of its protein carrier versican.
Enoxaparin Increases D6 Receptor Expression and Restores Cytoskeleton Organization in Trophoblast Cells from Preeclampsia
Authors: Tersigni Chiara , Maulucci Giuseppe , Castellani Roberta , Bianchetti Giada , Onori Marianna , Franco Rita , Barbaro Greta , De Spirito Marco , Lanzone Antonio , Scambia Giovanni , Di Simone Nicoletta
Published: June 2022

D6 is a scavenger receptor for CC chemokines expressed in the human placenta. It prevents excessive leukocyte tissue infiltration by internalizing chemokines through cytoskeleton-dependent intracellular transport. In preeclampsia (PE), the D6 receptor is overexpressed in trophoblast cells, but functionally impaired, due to cytoskeleton destructuring. Low molecular weight heparin (LMWH) represents a potential treatment for PE based on its anti-thrombotic and anti-inflammatory properties. Here, we investigated the effect of enoxaparin on D6 expression, and cytoskeleton organization primary cytotrophoblast cell cultures were obtained from the placentae of women with PE (n = 9) or uncomplicated pregnancy (n = 9). We demonstrated that enoxaparin is able to (i) increase D6 expression, and (ii) improve cytoskeletal fiber alignment in trophoblast cells from PE patients.
Characterization of Organellar-Specific ABA Responses during Environmental Stresses in Tobacco Cells and Arabidopsis Plants
Authors: Wang Yuzhu , Zhou Yeling , Liang Jiansheng
Published: June 2022

Abscisic acid (ABA) is a critical phytohormone involved in multifaceted processes in plant metabolism and growth under both stressed and nonstressed conditions. Its accumulation in various tissues and cells has long been established as a biomarker for plant stress responses. To date, a comprehensive understanding of ABA distribution and dynamics at subcellular resolution in response to environmental cues is still lacking. Here, we modified the previously developed ABA sensor ABAleon2.1_Tao3 (Tao3) and targeted it to different organelles including the endoplasmic reticulum (ER), chloroplast/plastid, and nucleus through the addition of corresponding signal peptides. Together with the cytosolic Tao3, we show distinct ABA distribution patterns in different tobacco cells with the chloroplast showing a lower level of ABA in both cell types. In a tobacco mesophyll cell, organellar ABA displayed specific alterations depending on osmotic stimulus, with ABA levels being generally enhanced under a lower and higher concentration of salt and mannitol treatment, respectively. In Arabidopsis roots, cells from both the meristem and elongation zone accumulated ABA considerably in the cytoplasm upon mannitol treatment, while the plastid and nuclear ABA was generally reduced dependent upon specific cell types. In Arabidopsis leaf tissue, subcellular ABA seemed to be less responsive when stressed, with notable increases of ER ABA in epidermal cells and a reduction of nuclear ABA in guard cells. Together, our results present a detailed characterization of stimulus-dependent cell type-specific organellar ABA responses in tobacco and Arabidopsis plants, supporting a highly coordinated regulatory network for mediating subcellular ABA homeostasis during plant adaptation processes.
Ferroptosis and Its Role in Chronic Diseases
Authors: Hu Wenli , Liang Kehong , Zhu Hong , Zhao Chong , Hu Hongbo , Yin Shutao
Published: June 2022

Ferroptosis, which has been widely associated with many diseases, is an iron-dependent regulated cell death characterized by intracellular lipid peroxide accumulation. It exhibits morphological, biochemical, and genetic characteristics that are unique in comparison to other types of cell death. The course of ferroptosis can be accurately regulated by the metabolism of iron, lipids, amino acids, and various signal pathways. In this review, we summarize the basic characteristics of ferroptosis, its regulation, as well as the relationship between ferroptosis and chronic diseases such as cancer, nervous system diseases, metabolic diseases, and inflammatory bowel diseases. Finally, we describe the regulatory effects of food-borne active ingredients on ferroptosis.
Priming with a Combination of FGF2 and HGF Restores the Impaired Osteogenic Differentiation of Adipose-Derived Stem Cells
Authors: Park Jeong Seop , Kim Doyoung , Hong Hyun Sook
Published: June 2022

Classical aging-associated diseases include osteoporosis, diabetes, hypertension, and arthritis. Osteoporosis causes the bone to become brittle, increasing fracture risk. Among the various treatments for fractures, stem cell transplantation is currently in the spotlight. Poor paracrine/differentiation capacity, owing to donor age or clinical history, limits efficacy. Lower levels of fibroblast growth factor 2 (FGF2) and hepatocyte growth factor (HGF) are involved in cell repopulation, angiogenesis, and bone formation in the elderly ADSCs (ADSC-E) than in the young ADSCs (ADSC-Y). Here, we study the effect of FGF2/HGF priming on the osteogenic potential of ADSC-E, determined by calcium deposition in vitro and ectopic bone formation in vivo. Age-induced FGF2/HGF deficiency was confirmed in ADSCs, and their supplementation enhanced the osteogenic differentiation ability of ADSC-E. Priming with FGF2/HGF caused an early shift of expression of osteogenic markers, including Runt-related transcription factor 2 (Runx-2), osterix, and alkaline phosphatase (ALP) during osteogenic differentiation. FGF2/HGF priming also created an environment favorable to osteogenesis by facilitating the secretion of bone morphogenetic protein 2 (BMP-2) and vascular endothelial growth factor (VEGF). Bone tissue of ADSC-E origin was observed in mice transplanted with FGF/HGF-primed ADSC-E. Collectively, FGF2/HGF priming could enhance the bone-forming capacity in ADSC-E. Therefore, growth factor-mediated cellular priming can enhance ADSC differentiation in bone diseases and thus contributes to the increased efficacy in vivo.
Direct Interaction of Avian Cryptochrome 4 with a Cone Specific G-Protein
Authors: Görtemaker Katharina , Yee Chad , Bartölke Rabea , Behrmann Heide , Voß Jan-Oliver , Schmidt Jessica , Xu Jingjing , Solovyeva Vita , Leberecht Bo , Behrmann Elmar , Mouritsen Henrik , Koch Karl-Wilhelm
Published: June 2022

Background: Night-migratory birds sense the Earth’s magnetic field by an unknown molecular mechanism. Theoretical and experimental evidence support the hypothesis that the light-induced formation of a radical-pair in European robin cryptochrome 4a (ErCry4a) is the primary signaling step in the retina of the bird. In the present work, we investigated a possible route of cryptochrome signaling involving the α-subunit of the cone-secific heterotrimeric G protein from European robin. Methods: Protein–protein interaction studies include surface plasmon resonance, pulldown affinity binding and Förster resonance energy transfer. Results: Surface plasmon resonance studies showed direct interaction, revealing high to moderate affinity for binding of non-myristoylated and myristoylated G protein to ErCry4a, respectively. Pulldown affinity experiments confirmed this complex formation in solution. We validated these in vitro data by monitoring the interaction between ErCry4a and G protein in a transiently transfected neuroretinal cell line using Förster resonance energy transfer. Conclusions: Our results suggest that ErCry4a and the G protein also interact in living cells and might constitute the first biochemical signaling step in radical-pair-based magnetoreception.
Impact of Intubator’s Training Level on First-Pass Success of Endotracheal Intubation in Acute Care Settings: A Four-Center Retrospective Study
Authors: Kim Jung-Heon , Jung Jae-Yun , Park Joong-Wan , Lee Se-Uk , Son Meong-Hi , Lee Jeong-Yong
Published: June 2022

(1) Background: First-pass success (FPS) of endotracheal intubation is more challenging in children than in adults. We aimed to identify factors associated with FPS of intubation in acute care settings. (2) Methods: We analyzed data of children aged <10 years who underwent intubation within ≤24 h of arrival at four Korean emergency departments (2016–2019). Variables were compared according to FPS. A logistic regression was performed to quantify the association of factors with FPS. An experienced intubator was defined as a senior resident or a specialist. (3) Results: Of 280 children, 169 (60.4%) had FPS. The children with FPS were older (median age, 23.0 vs. 11.0 months; p = 0.018), were less frequently in their infancy (36.1% vs. 50.5%; p = 0.017), and were less likely to have respiratory compromise (41.4% vs. 55.0%; p = 0.030). The children with FPS tended to be more often intubated by experienced intubators than those without FPS (87.0% vs. 78.4%; p = 0.057). Desaturation was rarer in those with FPS. Factors associated with FPS were experienced intubators (aOR, 1.93; 95% CI, 1.01–3.67) and children’s age ≥12 months (1.84; 1.13–3.02). (4) Conclusion: FPS of intubation can be facilitated by deploying or developing clinically competent intubators, particularly for infants, in acute care settings.
Identification of Genetic Causes in Mayer-Rokitansky-Küster-Hauser (MRKH) Syndrome: A Systematic Review of the Literature
Authors: Triantafyllidi Varvara Ermioni , Mavrogianni Despoina , Kalampalikis Andreas , Litos Michael , Roidi Stella , Michala Lina
Published: June 2022

Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome is a congenital condition characterizing females with absence of the uterus and part of the vagina. Several genetic defects have been correlated with the presence of MRKH; however, the exact etiology is still unknown due to the complexity of the genetic pathways implicated during the embryogenetic development of the Müllerian ducts. A systematic review (SR) of the literature was conducted to investigate the genetic causes associated with MRKH syndrome and Congenital Uterine Anomalies (CUAs). This study aimed to identify the most affected chromosomal areas and genes along with their associated clinical features in order to aid clinicians in distinguishing and identifying the possible genetic cause in each patient offering better genetic counseling. We identified 76 studies describing multiple genetic defects potentially contributing to the pathogenetic mechanism of MRKH syndrome. The most reported chromosomal regions and the possible genes implicated were: 1q21.1 (RBM8A gene), 1p31-1p35 (WNT4 gene), 7p15.3 (HOXA gene), 16p11 (TBX6 gene), 17q12 (LHX1 and HNF1B genes), 22q11.21, and Xp22. Although the etiology of MRKH syndrome is complex, associated clinical features can aid in the identification of a specific genetic defect.
The Relationship between Father–Child Rough-and-Tumble Play and Children’s Working Memory
Authors: Freeman Emily Elsa , Robinson Erin Louise
Published: June 2022

Rough-and-tumble play (RTP) between fathers and children has been linked to many social, emotional, and behavioural child outcomes, such as reduced aggression and increased self-regulation. This study extends our understanding of the importance of RTP to the development of the executive function, working memory. Father–child dyads (N = 30) were asked to play two RTP games that were videorecorded for later observational coding. Fathers were also asked to report the frequency with which they play RTP games with their child. Two measures of working memory were also collected. The working-memory index of the Wechsler Preschool and the Primary Scale of Intelligence—Fourth Edition were used to measure working-memory ability, and the working-memory subscale of the Behaviour Rating Inventory of Executive Function was used as a measure of working-memory problems. RTP frequency was associated with improved working-memory ability and fewer working-memory problems. RTP quality was associated with higher working-memory ability. This study adds to the growing evidence of the importance of father–child RTP for child development.
Self-Reported Psychosomatic Complaints and Conduct Problems in Swedish Adolescents
Authors: Brooks Samantha J. , Titova Olga E. , Ashworth Emma L. , Bylund Simon B. A. , Feldman Inna , Schiöth Helgi B.
Published: June 2022

Physical conditions in children and adolescents are often under reported during mainstream school years and may underlie mental health disorders. Additionally, comparisons between younger and older schoolchildren may shed light on developmental differences regarding the way in which physical conditions translate into conduct problems. The aim of the current study was to examine the incidence of psychosomatic complaints (PSC) in young and older adolescent boys and girls who also report conduct problems. A total of 3132 Swedish adolescents (age range 15–18 years, 47% boys) completed the Uppsala Life and Health Cross-Sectional Survey (LHS) at school. The LHS question scores were categorised by two researchers who independently identified questions that aligned with DSM-5 conduct disorder (CD) criteria and PSC. MANOVA assessed the effects of PSC, age, and gender on scores that aligned with the DSM criteria for CD. The main effects of gender, age, and PSC on the conduct problem scores were observed. Adolescents with higher PSC scores had higher conduct problem scores. Boys had higher serious violation of rules scores than girls, particularly older boys with higher PSC scores. Psychosomatic complaints could be a useful objective identifier for children and adolescents at risk of developing conduct disorders. This may be especially relevant when a reliance on a child’s self-reporting of their behavior may not help to prevent a long-term disturbance to their quality of life.
Dynamic Soil–Structure Interaction Effects in Buildings Founded on Vertical Reinforcement Elements
Authors: Jiménez Guillermo A. López , Dias Daniel
Published: June 2022

Pile foundation is an effective technique to support buildings in the presence of soft soil and seismic areas. More recently, the rigid inclusions system has also been utilized for founding buildings. Both systems increase the bearing capacity of the soil and allow reducing the total and differential settlements in the structure. However, the study of these systems in a complete and accurate way implies the consideration of the soil–structure interaction (SSI). In order to investigate the impact of different pile toe conditions (including the placement on hard soil, an anchorage and floating piles) in the response of mid-rise buildings, numerical models with a 5-storey frame building founded on the inclusions system (soil–inclusion–platform–structure) are analyzed and compared with the pile system (soil–pile–structure). Fully coupled finite difference numerical models were developed using Flac 3D. The influence of the dynamic characteristics of the structure was considered analyzing buildings with different heights (3 storeys to 7 storeys). The linear elastic perfectly plastic model with a Mohr–Coulomb failure criterion is used to represent the behavior of the soil. Values of the maximum lateral displacements, of the inter-storey drifts and of the shear forces distribution in the buildings, as well as the rocking of the foundation, are presented. Concerning the foundations, efforts and displacements are compared for the different systems. The results show that the type of support condition influences the seismic response of the building and the efforts and displacements in the rigid elements, depending on the foundation system. The efforts at the toe level in the rigid elements are highly influenced by the support conditions, but there is only a slight influence from the head connection.