bluuebunny/crossref_metadata_2025 huggingface dataset:
Depression and/or PTSD Comorbidity Affects Response to Antidepressants in Those With Alcohol Use Disorder
Authors: Na Peter J. , Ralevski Elizabeth , Jegede Oluwole , Wolfgang Aaron , Petrakis Ismene L.
Published: January 2022

Objective: Depression and post-traumatic stress disorder (PTSD) highly co-occur with alcohol use disorder (AUD). The comparative effects of noradrenergic vs. serotonergic antidepressants on drinking and depressive outcomes for those with AUD and co-occurring depression and/or PTSD are not well known.

Methods: This study was an analysis of a randomized control trial of 128 patients with AUD who had co-occurring depression and/or PTSD. They were randomized to treatment with paroxetine vs. desipramine and naltrexone vs. placebo leading to four groups: paroxetine plus naltrexone, paroxetine plus placebo, desipramine plus naltrexone, and desipramine plus placebo. Outcomes were percent of drinking days, percent heavy drinking days, drinks per drinking day (Time Line Follow-back Method), and depressive symptoms (Hamilton Depression Scale). Groups compared were (1) depression without PTSD (depression group; n = 35), (2) PTSD without depression (PTSD group; n = 33), and (3) both depression and PTSD (comorbid group; n = 60).

Results: There were no overall significant differences in drinking outcomes by medication in the entire sample, and no significant interaction when diagnostic groups were not considered. However, when diagnostic groups were included in the model, the interactions between time, diagnostic group, and medication (desipramine vs. paroxetine) were significant for percent drinking days (p = 0.042), and percent heavy drinking days (p = 0.036); paroxetine showed better drinking outcomes within the depression group, whereas desipramine showed better drinking outcomes in the PTSD and comorbid groups. Regarding depressive symptoms, paroxetine was statistically superior to desipramine in the total sample (p = 0.007), but there was no significant interaction of diagnostic group and medication. Naltrexone led to a decrease in craving but no change in drinking outcomes.

Conclusions: The results of this study suggest that drinking outcomes may respond differently to desipramine and paroxetine depending on comorbid MDD and/or PTSD.
Association Between Changes in Depressive Symptoms and Hazardous Drinking: Findings From the Korea Welfare Panel Study (2013–2018)
Authors: Jeong Sung Hoon , Lee Doo Woong , Kang Soo Hyun , Kim Seung Hoon , Park Eun-Cheol , Shin Jaeyong
Published: January 2022

Objective: This study aimed to evaluate the longitudinal association between changes in depressive symptoms and hazardous drinking among South Korean adults.

Participants/Methods: This study was conducted using a sample drawn from participants enrolled in the Korea Welfare Panel Study (KoWePS) from 2013 to 2018. Hazardous drinking was defined as a score of 11 points for men and seven points for women on the Korean version of the Alcohol Use Disorders Identification Test. Depressive symptoms were evaluated using an 11-item version of the Center for Epidemiologic Studies Depression Scale. A generalized estimating equation model was used to analyze the association between changes in depressive symptoms and hazardous drinking.

Results: Of the 12,878 people registered with KoWePS and without follow-up losses from 2013 to 2018, a total of 2,341 were included in this study, excluding those under the age of 19 and those with missing data. Persistent depressive symptoms (men, odds ratio [OR]: 2.20, 95% confidence interval [CI]: 1.48–3.21; women, OR: 2.64, 95% CI: 1.66–4.22) and the changes from non-depressive symptoms to depressive symptoms (men, adjusted OR: 2.18, 95% CI: 1.80–2.64; women, OR: 1.71, 95% CI: 1.27–2.29) increased the likelihood of hazardous drinking.

Conclusions: Persistent depressive symptoms and changes from non-depressive to depressive symptoms are associated with increased prevalence of hazardous drinking. This suggests that an evaluation of the underlying mental illness or emotion should be made when counseling for abstaining from alcohol for chronic drinkers or the general public.
Genomic Surveillance Enables the Identification of Co-infections With Multiple SARS-CoV-2 Lineages in Equatorial Guinea
Authors: Hosch Salome , Mpina Maxmillian , Nyakurungu Elizabeth , Borico Nelson Silochi , Obama Teodora Mikumu Alogo , Ovona Maria Carmen , Wagner Philipp , Rubin Sarah E. , Vickos Ulrich , Milang Diosdado Vicente Nsue , Ayekaba Mitoha Ondo'o , Phiri Wonder P. , Daubenberger Claudia A. , Schindler Tobias
Published: January 2022

COVID-19 disease caused by SARS-CoV-2 represents an ongoing global public health emergency. Rapid identification of emergence, evolution, and spread of SARS-CoV-2 variants of concern (VOC) would enable timely and tailored responses by public health decision-making bodies. Yet, global disparities in current SARS-CoV-2 genomic surveillance activities reveal serious geographical gaps. Here, we discuss the experiences and lessons learned from the SARS-CoV-2 monitoring and surveillance program at the Public Health Laboratory on Bioko Island, Equatorial Guinea that was implemented as part of the national COVID-19 response and monitoring activities. We report how three distinct SARS-CoV-2 variants have dominated the epidemiological situation in Equatorial Guinea since March 2020. In addition, a case of co-infection of two SARS-CoV-2 VOC, Beta and Delta, in a clinically asymptomatic and fully COVID-19 vaccinated man living in Equatorial Guinea is presented. To our knowledge, this is the first report of a person co-infected with Beta and Delta VOC globally. Rapid identification of co-infections is relevant since these might provide an opportunity for genetic recombination resulting in emergence of novel SARS-CoV-2 lineages with enhanced transmission or immune evasion potential.
Transcutaneous Electrical Spinal Cord Stimulation to Promote Recovery in Chronic Spinal Cord Injury
Authors: Tefertiller Candace , Rozwod Meghan , VandeGriend Eric , Bartelt Patricia , Sevigny Mitch , Smith Andrew C.
Published: January 2022

Objective: To evaluate the impact of using transcutaneous electrical spinal cord stimulation (TSCSTSCS) on upper and lower extremity function in individuals with chronic spinal cord injury (SCI).

Design: Prospective case series.

Setting: SCI specific rehabilitation hospital.

Participants: A convenience sample (N = 7) of individuals with tetraplegia who had previously been discharged from outpatient therapy due to a plateau in progress.

Interventions: Individuals participated in 60 min of upper extremity (UE) functional task-specific practice (FTP) in combination with TSCS and 60 min of locomotor training in combination with TSCS 5x/week.

Main Outcome Measures: The primary outcome for this analysis was the Capabilities of Upper Extremity Test (CUE-T). Secondary outcomes include UE motor score (UEMS), LE motor score (LEMS), sensation (light touch and pin prick), Nine-Hole Peg Test, 10 meter walk test, 6 min walk test, and 5 min stand test.

Results: Seven individuals (four motor complete; three motor incomplete) completed 20–80 sessions UE and LE training augmented with TSCS and without any serious adverse events. Improvements were reported on the CUE-T in all seven individuals. Two individuals improved their ASIA impairment scale (AIS) classification (B to C; C to D) and two individuals improved their neurologic level of injury by one level (C4–C5; C5–C6). Sensation improved in five individuals and all four who started out with motor complete SCIs were able to voluntarily activate their LEs on command in the presence of stimulation.

Conclusion: Individuals with chronic SCI who had previously demonstrated a plateau in function after an intensive outpatient therapy program were able to improve in a variety of UE and LE outcomes in response to TSCS without any adverse events. This was a small pilot study and future fully powered studies with comparative interventions need to be completed to assess efficacy.
Exoskeleton Training and Trans-Spinal Stimulation for Physical Activity Enhancement After Spinal Cord Injury (EXTra-SCI): An Exploratory Study
Authors: Sutor Tommy W. , Ghatas Mina P. , Goetz Lance L. , Lavis Timothy D. , Gorgey Ashraf S.
Published: January 2022

After spinal cord injury (SCI) physical activity levels decrease drastically, leading to numerous secondary health complications. Exoskeleton-assisted walking (EAW) may be one way to improve physical activity for adults with SCI and potentially alleviate secondary health complications. The effects of EAW may be limited, however, since exoskeletons induce passive movement for users who cannot volitionally contribute to walking. Trans-spinal stimulation (TSS) has shown the potential to enable those with even the most severe SCI to actively contribute to movements during EAW. To explore the effects of EAW training on improving secondary health complications in persons with SCI, participants with chronic (n = 8) were enrolled in an EAW program 2–3 times per week for 12 weeks. Anthropometrics (seated and supine waist and abdominal circumferences (WC and AC), body composition assessment (dual exposure x-ray absorptiometry-derived body fat percent, lean mass and total mass for the total body, legs, and trunk), and peak oxygen consumption (VO2 during a 6-minute walk test [6MWT]) were assessed before and after 12 weeks of EAW training. A subset of participants (n = 3) completed EAW training with concurrent TSS, and neuromuscular activity of locomotor muscles was assessed during a 10-m walk test (10MWT) with and without TSS following 12 weeks of EAW training. Upon completion of 12 weeks of training, reductions from baseline (BL) were found in seated WC (−2.2%, P = 0.036), seated AC (−2.9%, P = 0.05), and supine AC (−3.9%, P = 0.017). Percent fat was also reduced from BL for the total body (−1.4%, P = 0.018), leg (−1.3%, P = 0.018), and trunk (−2%, P = 0.036) regions. No effects were found for peak VO2. The addition of TSS for three individuals yielded individualized responses but generally increased knee extensor activity during EAW. Two of three participants who received TSS were also able to initiate more steps without additional assistance from the exoskeleton during a 10MWT. In summary, 12 weeks of EAW training significantly attenuated markers of obesity relevant to cardiometabolic health in eight men with chronic SCI. Changes in VO2 and neuromuscular activity with vs. without TSS were highly individualized and yielded no overall group effects.
Does the Correspondence Bias Apply to Social Robots?: Dispositional and Situational Attributions of Human Versus Robot Behavior
Authors: Edwards Autumn , Edwards Chad
Published: January 2022

Increasingly, people interact with embodied machine communicators and are challenged to understand their natures and behaviors. The Fundamental Attribution Error (FAE, sometimes referred to as the correspondence bias) is the tendency for individuals to over-emphasize personality-based or dispositional explanations for other people’s behavior while under-emphasizing situational explanations. This effect has been thoroughly examined with humans, but do people make the same causal inferences when interpreting the actions of a robot? As compared to people, social robots are less autonomous and agentic because their behavior is wholly determined by humans in the loop, programming, and design choices. Nonetheless, people do assign robots agency, intentionality, personality, and blame. Results of an experiment showed that participants made correspondent inferences when evaluating both human and robot speakers, attributing their behavior to underlying attitudes even when it was clearly coerced. However, they committed a stronger correspondence bias in the case of the robot–an effect driven by the greater dispositional culpability assigned to robots committing unpopular behavior–and they were more confident in their attitudinal judgments of robots than humans. Results demonstrated some differences in the global impressions of humans and robots based on behavior valence and choice. Judges formed more generous impressions of the robot agent when its unpopular behavior was coerced versus chosen; a tendency not displayed when forming impressions of the human agent. Implications of attributing robot behavior to disposition, or conflating robot actors with their actions, are addressed.
COVID-19 and Its Implications to the Assessment of Sustainable Palm Oil Supply Chain Management: An Indonesian Perspective
Authors: Hafiz Nusrat , Azmi Khairunnisa Mohd , Nimfa Danjuma Tali , Latiff Ahmad Shaharudin Abdul , Wahab Sazali Abd
Published: January 2022

Motivated by the low sustainability index and pressure to meet the global demand for eco-friendly crude palm oil (CPO) in the pandemic-ridden environment, this research aims to investigate the implications of the COVID-19 pandemic to assess the drivers of sustainable supply chain management (SSCM) of the Indonesian CPO sector to tackle supply chain disruptions. To achieve this aim, the study seeks to determine the sustainability drivers to accommodate the pandemic-ridden environment and if sustainability indicators can help improve the supply chain management of the CPO sector. A methodology is divided into two interrelated parts: first, based on a careful review of extant literature of the CPO sector and sustainable supply chain in the light of pandemic. The proposed methodology is then tested using the response data of 108 oil mills' representatives collected through survey questionnaires and analyzed using statistical tools of reliability, distribution, Kaiser–Meyer–Olkin (KMO), Confirmatory Factor Analysis (CFA), and diagnostic tests of CFA. The findings designate the environmental costs, rapidity, and adaptability as core economic indicators; the social and workforce development, health, and safety workforce development and consumer issues as crucial social indicators; while energy and material efficiency, management of waste and emissions, and sustainable suppliers as the best environmental indicators. This study provides a holistic platform on the implications of the pandemic to assess the SSCM of the CPO sector. These findings are expected to aid the industrial managers in employee skills and health protocols, customer service, and environmental management. The study is also anticipated to guide the supply-chain partners and government policymakers to take initiatives on SSCM in the context of the pandemic.
Moving Toward a More Comprehensive Analysis of Acceleration Profiles in Elite Youth Football
Authors: Kovacevic Damian , Elias George , Ellens Susanne , Cox Adam , Serpiello Fabio R.
Published: January 2022

In football, having greater acceleration ability may decide the most important moments within matches. Up to now, commonly used acceleration variables have typically been investigated in isolation, with each variable suffering from unique limitations. Subsequently, any findings may provide a limited representation of what specific acceleration demands had actually occurred. Without gaining a comprehensive understanding of acceleration demands in football, it appears difficult to identify how to best monitor and maximize the long-term development of acceleration ability in footballers, all whilst doing so in a safe, sport-specific manner. Moving toward a more comprehensive analysis of acceleration profiles addresses this, as it can provide a more robust, informative understanding of the unique acceleration demands of competitive match-play. This perspective article aims to discuss the benefits of adopting a more comprehensive analysis of the acceleration demands during competitive matches for football players, by simultaneously analyzing high-intensity accelerations, repeated high acceleration ability (RHAA), and average acceleration. We discuss examples of the calculation and application of a more comprehensive acceleration profile at a team level throughout the course of an entire elite youth football season, as well as on an individual level. Monitoring acceleration profiles more comprehensively not only appears important from a training load/injury prevention perspective, but also, equips coaches and conditioning staff with the specific information necessary to develop and prescribe individualized, acceleration-emphasized training protocols that are replicable to the demands of match-play. Examples of such protocols are provided.
Meta-analysis of Percutaneous vs. Surgical Approaches Radiofrequency Ablation in Hepatocellular Carcinoma
Authors: Huang Xiaozhun , Liu Yibin , Xu Lin , Ma Teng , Yin Xin , Huang Zhangkan , Wang Caibin , Huang Zhen , Bi Xinyu , Che Xu
Published: January 2022

Background: Radiofrequency ablation (RFA) is a curative modality for hepatocellular carcinoma (HCC) patients who are not suitable for resection. It remains controversial whether a surgical or percutaneous approach is more appropriate for HCC.

Method: A search was performed on the PubMed, Web of Science, Embase, and Cochrane Library databases from the date of database inception until April 17, 2021. Studies reporting outcomes of comparisons between surgical RFA (SRFA) and percutaneous RFA (PRFA) were included in this study. The meta-analysis was performed using the Review Manager 5.3 and Stata 12.0 software.

Result: A total of 10 retrospective studies containing 12 cohorts, involving 740 patients in the PRFA group and 512 patients in the SRFA group, were selected. Although the tumor size in PRFA group was smaller than the SRFA group (p = 0.007), there was no significant difference in complete ablation rate between the SRFA and PRFA groups (95.63% and 97.33%, respectively; Odds ratio [OR], 0.56; 95% confidence intervals [CI], 0.26–1.24; p = 0.15). However, the SRFA group showed a significantly lower local tumor recurrence than the PRFA group in the sensitivity analysis (28.7% in the PRFA group and 21.79% in the SRFA group, respectively; OR, 1.84; 95% CI, 1.14–2.95; p = 0.01). Pooled analysis data showed that the rate of severe perioperative complications did not differ significantly between the PRFA and SRFA groups (14.28% and 12.11%, respectively; OR, 1.30; 95% CI, 0.67-2.53; p = 0.44). There was no significant difference in the 1-, 3-, and 5-year overall survival rates, as well as the 1- and 3-year disease-free survival (DFS) between the PRFA and SRFA groups. The 5-year DFS of the PRFA group was significantly lower than the SRFA group (hazard ratio 0.73; 95% CI 0.54–0.99).

Conclusion: Based on our meta-analysis, the surgical route was superior to PRFA in terms of local control rate. Furthermore, the surgical approach did not increase the risk of major complications.
Adult Combined Heart-Liver Transplantation: The United States Experience
Authors: Alexopoulos Sophoclis P. , Wu W. Kelly , Ziogas Ioannis A. , Matsuoka Lea K. , Rauf Muhammad A. , Izzy Manhal , Perri Roman , Schlendorf Kelly H. , Menachem Jonathan N. , Shah Ashish S.
Published: January 2022

Background: We aimed to review the indications and outcomes of adults undergoing combined heart-liver transplantation (CHLT) in the US using national registry data.

Methods: Adult (≥18 years) CHLT recipients in the United Network for Organ Sharing database were included (09/1987–09/2020; era 1 = 1989–2000, era 2 = 2001–2010, era 3 = 2011–2020). Survival analysis was conducted by means of Kaplan-Meier method, log-rank test, and Cox regression.

Results: We identified 369 adults receiving CHLT between 12/1989–08/2020. The number of adult CHLT recipients (R2 = 0.75, p < 0.001) and centers performing CHLT (R2 = 0.80, p < 0.001) have increased over the study period. The most common cardiac diagnosis in the first two eras was restrictive/infiltrative cardiomyopathy, while the most common in era 3 was congenital heart disease (p = 0.03). The 1-, 3-, and 5-years patient survival was 86.8, 80.1, and 77.9%, respectively. In multivariable analysis, recipient diabetes [adjusted hazard ratio (aHR) = 2.35, 95% CI: 1.23–4.48], CHLT between 1989-2000 compared with 2011–2020 (aHR = 5.00, 95% CI: 1.13–22.26), and sequential-liver first CHLT compared with sequential-heart first CHLT (aHR = 2.44, 95% CI: 1.15–5.18) were associated with increased risk of mortality. Higher left ventricular ejection fraction was associated with decreased risk of mortality (aHR = 0.96, 95% CI: 0.92–0.99).

Conclusion: CHLT is being increasingly performed with evolving indications. Excellent outcomes can be achieved with multidisciplinary patient and donor selection and surgical planning.
Simple Analytical Strategy for Screening Three Synthetic Cathinones (α-PVT, α-PVP, and MDPV) in Oral Fluids
Authors: Segurado André M. , Ahmad Samir M. , Neng Nuno R. , Maniés-Sequeira Margarida M. , Gaspar Helena , Nogueira José Manuel F.
Published: January 2022

Synthetic cathinones are analogue compounds of the plant based stimulant cathinone. Its use, abuse, and related consumption complications have steadily increased in the last years. For this reason, there is a need for innovative analytical approaches that enable its rapid screening in biological matrices (e.g., oral fluids). The present work proposes a new analytical methodology by combining bar adsorptive microextraction followed by microliquid desorption and gas chromatography coupled to mass spectrometry (BAµE-µLD/GC-MS) for screening three synthetic cathinones (α-PVP, α-PVT, and MDPV) in oral fluids. The optimization of the BAµE-µLD/GC-MS methodology was successfully applied for the analysis of the target compounds in oral fluids. The results show average recoveries between 43.1 and 52.3% for the three synthetic cathinones. Good selectivity was also noticed. The developed methodology presents itself as an alternative tool to screen these compounds in oral fluids. To the best of our knowledge, this is the first work that combines a microextraction sorption-based technique followed by GC-MS analysis for the screening of synthetic cathinones in oral fluids.
Phytochemical Analysis and Dermo-Cosmetic Evaluation of Cymbidium sp. (Orchidaceae) Cultivation By-Products
Authors: Axiotis Evangelos , Angelis Apostolis , Antoniadi Lemonia , Petrakis Eleftherios A. , Skaltsounis Leandros A.
Published: January 2022

Cymbidium is one of the most popular genera in Orchidaceae family, commercialized either as loose flowers or as potted plants in floriculture worldwide. The non-marketable parts are typically discarded (e.g., unsuitable flowers, leaves, pseudobulbs, roots), generating an enormous quantity of unutilized biomass. The above by-products were studied through phytochemical analysis and investigated for their dermo-cosmetic potential. The initial antioxidant, anti-tyrosinase, anti-elastase, and anti-collagenase assays of the total extracts indicated that the pseudobulb and root ethyl acetate extracts were the most potent. Those extracts were then submitted to chromatographic separation leading to the isolation of 16 secondary metabolites (four phenanthrenes, three 1,4-phenanthrenquinones, three dibenzyls, two phenolic acid derivatives, two sterols, one dehydrodiconiferyl alcohol derivative, and one simple phenolic compound), including 6-hydroxy-5,7-dimethoxy-1,4-phenanthrenequinone (cymbisamoquinone), which was identified as a new natural product. In parallel, 48 metabolites were identified by UPLC-HRMS analysis of the extracts. The biological evaluation of the isolated compounds revealed that gigantol and tristin present important anti-tyrosinase activity, while bulbophyllanthrin, 3-hydroxy-2,4,7-trimethoxy-phenanthrene, marylaurencinol A, 5-hydroxy-2-methoxy-1,4-phenanthrenequinone, and ephemeranthroquinone B show dose-dependent anti-collagenase activity. In contrast to isolated metabolites, which may act selectively on specific enzymes, the initial total extracts exhibited inhibitory activity against tyrosinase, elastase, and collagenase enzymes, thus showing better prospects for use in dermo-cosmetic formulations.
Anti-Allergic Effects of Myrciaria dubia (Camu-Camu) Fruit Extract by Inhibiting Histamine H1 and H4 Receptors and Histidine Decarboxylase in RBL-2H3 Cells
Authors: Do Nhung Quynh , Zheng Shengdao , Oh Sarang , Nguyen Quynh T. N. , Fang Minzhe , Kim Minseon , Choi Junhui , Kim Myeong-Ju , Jeong Jeehaeng , Yi Tae-Hoo
Published: January 2022

Although Myrciaria dubia (camu-camu) has been shown to exert anti-oxidant and anti-inflammatory effects in both in vitro and in vivo studies, its use in allergic responses has not been elucidated. In the present study, the anti-allergic effect of 70% ethanol camu-camu fruit extract was tested on calcium ionophore (A23187)-induced allergies in RBL-2H3 cells. The RBL-2H3 cells were induced with 100 nM A23187 for 6 h, followed by a 1 h camu-camu fruit extract treatment. A23187 sanitization exacerbated mast cell degranulation; however, camu-camu fruit extract decreased the release of histamine and β-hexosaminidase, which are considered as key biomarkers in cell degranulation. Camu-camu fruit extract inhibited cell exocytosis by regulating the calcium/nuclear factor of activated T cell (NFAT) signaling. By downregulating the activation of mitogen-activated protein kinase (MAPK) signaling, camu-camu fruit extract hindered the activation of both histamine H1 and H4 receptors and inhibited histidine decarboxylase (HDC) expression by mediating its transcription factors KLF4/SP1 and GATA2/MITF. In A23187-induced ROS overproduction, camu-camu fruit extract activated nuclear factor erythroid-2-related factor 2 (Nrf2) to protect mast cells against A23187-induced oxidative stress. These findings indicate that camu-camu fruit extract can be developed to act as a mast cell stabilizer and an anti-histamine. This work also “opens the door” to new investigations using natural products to achieve breakthroughs in allergic disorder treatment.
Fatty Acid Oxidation and Pro-Resolving Lipid Mediators Are Related to Male Infertility
Authors: Signorini Cinzia , Moretti Elena , Noto Daria , Micheli Lucia , Ponchia Rosetta , Collodel Giulia
Published: January 2022

Specialized pro-resolving lipid mediators regulate the resolution of acute inflammation. They are formed by enzymatic oxygenation of polyunsaturated fatty acids and are divided into families including lipoxins, resolvins, protectins, and maresins. Resolvin D1 (RvD1), produced by docosahexaenoic acid, exerts anti-inflammatory and pro-resolving activities. This research aimed to investigate the implication of seminal RvD1 in human infertility. Infertile patients (n° 67) were grouped based on pathological reproductive conditions as idiopathic infertility, varicocele, and leukocytospermia; the fourth group was composed of fertile men (n° 18). Sperm characteristics were evaluated by light microscopy (WHO guidelines) and by transmission electron microscopy (TEM). The seminal levels of RvD1 and F2-isoprostane (F2-IsoPs) were dosed. In twenty men (6 fertile men, 8 with varicocele, 6 with leukocytospermia) seminal phospholipase A2, iron, cholesterol, transferrin, estradiol, ferritin, testosterone, and sperm membrane fatty acids were detected. The results indicated that: (i) RvD1 amount was positively correlated with F2-IsoPs and reduced sperm quality; (ii) RvD1 levels were significantly higher in patients with leukocytospermia, varicocele, and idiopathic infertility compared to fertile men; (iii) RvD1 increased along with other markers of oxidative stress and inflammation as fatty acids content and clinical biomarkers. This study suggests a panel of inflammatory markers and lipid mediators for a diagnosis of inflammatory status and a subsequent appropriate therapeutic approach.
Dynamic Wheel-Rail Force-Based Track-Irregularity Evaluation for Ballasted Track on Serviced Railway by Adjacent Excavation
Authors: Choi Jung-Youl , Park Dong-Ryong , Chung Jee-Seung , Kim Sun-Hee
Published: January 2022

This study investigates a load-based, track-irregularity-analysis technique for ballasted tracks on a serviced railway line with respect to excavation work conducted in adjacent sites. A numerical analysis and field measurements (railbed-settlement-monitoring sensor, track-geometry-measurement system, wheel-load measurements) were analyzed comparatively to demonstrate the correlation between the track irregularities and the Wheel-Rail interaction force. In this way, we highlight the necessity for load-based track-irregularity-management methods. The analyzed results show that the maximum dynamic wheel load was measured in the range of approximately 10 m before and after the location where the maximum track irregularities occurred, and that even if the maintenance criteria of track irregularities were satisfied, the design dynamic wheel load could still be exceeded depending on the train speed, thus indicating that track damage can be caused by the impact load.
MSC-Net: Multitask Learning Network for Retinal Vessel Segmentation and Centerline Extraction
Authors: Pan Lin , Zhang Zhen , Zheng Shaohua , Huang Liqin
Published: January 2022

Automatic segmentation and centerline extraction of blood vessels from retinal fundus images is an essential step to measure the state of retinal blood vessels and achieve the goal of auxiliary diagnosis. Combining the information of blood vessel segments and centerline can help improve the continuity of results and performance. However, previous studies have usually treated these two tasks as separate research topics. Therefore, we propose a novel multitask learning network (MSC-Net) for retinal vessel segmentation and centerline extraction. The network uses a multibranch design to combine information between two tasks. Channel and atrous spatial fusion block (CAS-FB) is designed to fuse and correct the features of different branches and different scales. The clDice loss function is also used to constrain the topological continuity of blood vessel segments and centerline. Experimental results on different fundus blood vessel datasets (DRIVE, STARE, and CHASE) show that our method can obtain better segmentation and centerline extraction results at different scales and has better topological continuity than state-of-the-art methods.
Changes in the Medieval Colour Scheme of the Southern Façade of Wrocław Town Hall—A Case Study
Authors: Legendziewicz Andrzej , Marcinów Aleksandra
Published: January 2022

The aim of this article is to discuss the colour transformations of one of the most representative Gothic façades in Central Europe—the southern façade of Wrocław Town Hall. Based on iconographic, architectural, and stratigraphic research, it was possible to find the remains of two stages of medieval colour changes. Based on these discoveries, an attempt was made to reconstruct both phases of the medieval colour scheme. The research findings enable the object to be described with regard to the architecture and colours of late gothic façades in Poland, Czechia and Germany.
WWLLN Hot and Cold-Spots of Lightning Activity and Their Relation to Climate in an Extended Central America Region 2012–2020
Authors: Amador Jorge A. , Arce-Fernández Dayanna
Published: January 2022

Lightning activity has been recognized to have, historically, social and environmental consequences around the globe. This work analyzes the space-time distribution of lightning-densities (D) in an extended Central America region (ECA). World Wide Lightning Location Network data was analyzed to link D with dominant climate patterns over the ECA for 2012–2020. D associated with cold surges entering the tropics dominate during boreal winter. The highest D (hot-spots) was found to agree well with previously known sites, such as the “Catatumbo” in Venezuela; however, D was lower here due to different detection efficiencies. Previously reported hot-spots showed strong continental signals in CA; however, in this work, they were over the oceans near to coastlines, especially in the eastern tropical Pacific (ETP). Most cold-spots, implying a minimum of vulnerability to human impacts and to some industries, were situated in the Caribbean Sea side of Central America. The Mid-Summer-Drought and the Caribbean-Low-Level-Jet (CLLJ) markedly reduced the D during July-August. The CLLJ in the central CS and across the Yucatan and the southern Gulf of Mexico acts as a lid inhibiting convection due to its strong vertical shear during the boreal summer. The CLLJ vertical wind-shear and its extension to the Gulf of Papagayo also diminished convection and considerably decreased the D over a region extending westward into the ETP for at least 400–450 km. A simple physical mechanism to account for the coupling between the CLLJ, the MSD, and lightning activity is proposed for the latter region.
A Muscle-Driven Mechanism for Locomotion of Snake-Robots
Authors: Lopez Marcela , Haghshenas-Jaryani Mahdi
Published: January 2022

This paper presents the concept of muscle-driven locomotion for planar snake robots, which combines the advantages of both rigid and soft robotic approaches to enhance the performance of snake robot locomotion. For this purpose, two adjacent links are connected by a pair of pneumatic artificial muscles wherein an alternate actuation of these soft actuators causes a rotational motion at the connecting joints. The muscle-based actuated linkage mechanism, as a closed six-linkage mechanism, was designed and prototyped. The linear motion and force generation of the pneumatic artificial muscle was experimentally characterized using isotonic and isometric contraction experiments. A predictive model was developed based on the experimental data to describe the relationship between the force–length–pressure of the PAMs. Additionally, the muscle-driven mechanism was kinematically and dynamically characterized based on both theoretical and experimental studies. The experimental data generally agreed with our model’s results and the generated joint angle and torque were comparable to the current snake-like robots. A skx-link planar snake robot with five joints, five pairs of antagonistic muscles, and an associated pneumatic controller was prototyped and examined for simple movements on a straight-line. We demonstrated the muscle-driven locomotion of the six-link snake robot, and the results show the feasibility of using the proposed mechanism for future explorations of snake robot locomotion.
Engineered Dutasteride-Lipid Based Nanoparticle (DST-LNP) System Using Oleic and Stearic Acid for Topical Delivery
Authors: Noor Norhayati Mohamed , Umar Sana , Abdul-Aziz Azila , Sheikh Khalid , Somavarapu Satyanarayana
Published: January 2022

Male pattern baldness (MPB) is a common condition that has a negative impact on the psycho-social health of many men. This study aims to engineer an alcohol-free formulation to cater for individuals who may have had allergic reactions to alcohol-based preparations. A lipid-based nanoparticle system composed of stearic and oleic acid (solid and liquid lipid) was used to deliver dutasteride (DST) for topical application. Two compositions, with oleic acid (Formulation A) and without (Formulation B), were compared to analyse the role of oleic acid as a potential active ingredient in addition to DST. DST-loaded LNP were prepared using the emulsification–ultrasonication method. All of the prepared formulations were spherical in shape in the nanometric size range (150–300 nm), with entrapment efficiencies of >75%. X-ray diffractograms revealed that DST exists in an amorphous form within the NLP matrices. The drug release behaviour from both LNP preparations displayed slow release of DST. Permeation studies through pig ear skin demonstrated that DST-LNP with oleic acid produced significantly lower permeation into the dermis compared to the formulation without oleic acid. These results suggest that the proposed formulation presents several characteristics which are novel, indicating its suitability for the dermal delivery of anti-androgenic molecules.
The Functional Differences between the GroEL Chaperonin of Escherichia coli and the HtpB Chaperonin of Legionella pneumophila Can Be Mapped to Specific Amino Acid Residues
Authors: Valenzuela-Valderas Karla N. , Moreno-Hagelsieb Gabriel , Rohde John R. , Garduño Rafael A.
Published: January 2022

Group I chaperonins are a highly conserved family of essential proteins that self-assemble into molecular nanoboxes that mediate the folding of cytoplasmic proteins in bacteria and organelles. GroEL, the chaperonin of Escherichia coli, is the archetype of the family. Protein folding-independent functions have been described for numerous chaperonins, including HtpB, the chaperonin of the bacterial pathogen Legionella pneumophila. Several protein folding-independent functions attributed to HtpB are not shared by GroEL, suggesting that differences in the amino acid (aa) sequence between these two proteins could correlate with functional differences. GroEL and HtpB differ in 137 scattered aa positions. Using the Evolutionary Trace (ET) bioinformatics method, site-directed mutagenesis, and a functional reporter test based upon a yeast-two-hybrid interaction with the eukaryotic protein ECM29, it was determined that out of those 137 aa, ten (M68, M212, S236, K298, N507 and the cluster AEHKD in positions 471-475) were involved in the interaction of HtpB with ECM29. GroEL was completely unable to interact with ECM29, but when GroEL was modified at those 10 aa positions, to display the HtpB aa, it acquired a weak ability to interact with ECM29. This constitutes proof of concept that the unique functional abilities of HtpB can be mapped to specific aa positions.
Endothelial Dysfunction: From a Pathophysiological Mechanism to a Potential Therapeutic Target
Authors: Ambrosino Pasquale , Grassi Guido , Maniscalco Mauro
Published: January 2022

The endothelium is considered the largest organ of the body, composed of a monolayer of endothelial cells (ECs) lining the interior surface of blood and lymphatic vessels [...]
Sweet Taste Preference: Relationships with Other Tastes, Liking for Sugary Foods and Exploratory Genome-Wide Association Analysis in Subjects with Metabolic Syndrome
Authors: Fernández-Carrión Rebeca , Sorlí Jose V. , Coltell Oscar , Pascual Eva C. , Ortega-Azorín Carolina , Barragán Rocío , Giménez-Alba Ignacio M. , Alvarez-Sala Andrea , Fitó Montserrat , Ordovas Jose M. , Corella Dolores
Published: January 2022

Taste perception and its association with nutrition and related diseases (type 2 diabetes, obesity, metabolic syndrome, cardiovascular, etc.) are emerging fields of biomedicine. There is currently great interest in investigating the environmental and genetic factors that influence sweet taste and sugary food preferences for personalized nutrition. Our aims were: (1) to carry out an integrated analysis of the influence of sweet taste preference (both in isolation and in the context of other tastes) on the preference for sugary foods and its modulation by type 2 diabetes status; (2) as well as to explore new genetic factors associated with sweet taste preference. We studied 425 elderly white European subjects with metabolic syndrome and analyzed taste preference, taste perception, sugary-foods liking, biochemical and genetic markers. We found that type 2 diabetic subjects (38%) have a small, but statistically higher preference for sweet taste (p = 0.021) than non-diabetic subjects. No statistically significant differences (p > 0.05) in preferences for the other tastes (bitter, salty, sour or umami) were detected. For taste perception, type 2 diabetic subjects have a slightly lower perception of all tastes (p = 0.026 for the combined “total taste score”), bitter taste being statistically lower (p = 0.023). We also carried out a principal component analysis (PCA), to identify latent variables related to preferences for the five tastes. We identified two factors with eigenvalues >1. Factor 2 was the one with the highest correlation with sweet taste preference. Sweet taste preference was strongly associated with a liking for sugary foods. In the exploratory SNP-based genome-wide association study (GWAS), we identified some SNPs associated with sweet taste preference, both at the suggestive and at the genome-wide level, especially a lead SNP in the PTPRN2 (Protein Tyrosine Phosphatase Receptor Type N2) gene, whose minor allele was associated with a lower sweet taste preference. The PTPRN2 gene was also a top-ranked gene obtained in the gene-based exploratory GWAS analysis. In conclusion, sweet taste preference was strongly associated with sugary food liking in this population. Our exploratory GWAS identified an interesting candidate gene related with sweet taste preference, but more studies in other populations are required for personalized nutrition.
Alterations in the Proteome and Phosphoproteome Profiles of Rat Hippocampus after Six Months of Morphine Withdrawal: Comparison with the Forebrain Cortex
Authors: Ujcikova Hana , Eckhardt Adam , Hejnova Lucie , Novotny Jiri , Svoboda Petr
Published: January 2022

The knowledge about proteome changes proceeding during protracted opioid withdrawal is lacking. Therefore, the aim of this work was to analyze the spectrum of altered proteins in the rat hippocampus in comparison with the forebrain cortex after 6-month morphine withdrawal. We utilized 2D electrophoretic workflow (Pro-Q® Diamond staining and Colloidal Coomassie Blue staining) which was preceded by label-free quantification (MaxLFQ). The phosphoproteomic analysis revealed six significantly altered hippocampal (Calm1, Ywhaz, Tuba1b, Stip1, Pgk1, and Aldoa) and three cortical proteins (Tubb2a, Tuba1a, and Actb). The impact of 6-month morphine withdrawal on the changes in the proteomic profiles was higher in the hippocampus—14 proteins, only three proteins were detected in the forebrain cortex. Gene Ontology (GO) enrichment analysis of differentially expressed hippocampal proteins revealed the most enriched terms related to metabolic changes, cytoskeleton organization and response to oxidative stress. There is increasing evidence that energy metabolism plays an important role in opioid addiction. However, the way how morphine treatment and withdrawal alter energy metabolism is not fully understood. Our results indicate that the rat hippocampus is more susceptible to changes in proteome and phosphoproteome profiles induced by 6-month morphine withdrawal than is the forebrain cortex.
Oxidative Stress and Antioxidant Nanotherapeutic Approaches for Inflammatory Bowel Disease
Authors: Liu Ping , Li Yixuan , Wang Ran , Ren Fazheng , Wang Xiaoyu
Published: January 2022

Oxidative stress, caused by the accumulation of reactive species, is associated with the initiation and progress of inflammatory bowel disease (IBD). The investigation of antioxidants to target overexpressed reactive species and modulate oxidant stress pathways becomes an important therapeutic option. Nowadays, antioxidative nanotechnology has emerged as a novel strategy. The nanocarriers have shown many advantages in comparison with conventional antioxidants, owing to their on-site accumulation, stability of antioxidants, and most importantly, intrinsic multiple reactive species scavenging or catalyzing properties. This review concludes an up-to-date summary of IBD nanomedicines according to the classification of the delivered antioxidants. Moreover, the concerns and future perspectives in this study field are also discussed.
The Evidence for Sparsentan-Mediated Inhibition of INa and IK(erg): Possibly Unlinked to Its Antagonism of Angiotensin II or Endothelin Type a Receptor
Authors: Chuang Tzu-Hsien , Cho Hsin-Yen , Wu Sheng-Nan
Published: January 2022

Sparsentan is viewed as a dual antagonist of endothelin type A (ETA) receptor and angiotensin II (AngII) receptor and it could be beneficial in patients with focal segmental glomerulosclerosis. Moreover, it could improve glomerular filtration rate and augment protective tissue remodeling in mouse models of focal segmental glomerulosclerosis. The ionic mechanisms through which it interacts with the magnitude and/or gating kinetics of ionic currents in excitable cells were not thoroughly investigated. Herein, we aimed to examine the effects of varying sparsentan concentrations on ionic currents residing in pituitary GH3 somatolactotrophs. From whole-cell current recordings made in GH3 cells, sparsentan (0.3–100 μM) differentially inhibited the peak and late components of voltage-gated Na+ current (INa). The IC50 value of sparsentan required to exert a reduction in peak and late INa in GH3 cells was 15.04 and 1.21 μM, respectively; meanwhile, the KD value estimated from its shortening in the slow component of INa inactivation time constant was 2.09 μM. The sparsentan (10 μM) presence did not change the overall current–voltage relationship of INa; however, the steady-state inactivation curve of the current was shifted to more negative potential in its presence (10 μM), with no change in the gating charge of the curve. The window INa activated by a brief upsloping ramp was decreased during exposure to sparsentan (10 μM); moreover, recovery of peak INa became slowed in its presence. The Tefluthrin (Tef)-stimulated resurgent INa activated in response to abrupt depolarization followed by the descending ramp pulse was additionally attenuated by subsequent application of sparsentan. In continued presence of Tef (3 μM) or β-pompilidotoxin (3 μM), further application of sparsentan (3 μM) reversed their stimulation of INa. However, sparsentan-induced inhibition of INa failed to be overcome by subsequent application of either endothelin 1 (1 μM) or angiotensin II (1 μM); moreover, in continued presence of endothelin (1 μM) or angiotensin II (1 μM), further addition of sparsentan (3 μM) effectively decreased peak INa. Additionally, the application of sparsentan (3 μM) inhibited the peak and late components of erg-mediated K+ current in GH3 cells, although it mildly decreased the amplitude of delayed-rectifier K+ current. Altogether, this study provides a distinct yet unidentified finding that sparsentan may perturb the amplitude or gating of varying ionic currents in excitable cells.
The Role of Circulating Biomarkers in the Oncological Management of Metastatic Renal Cell Carcinoma: Where Do We Stand Now?
Authors: Cinque Alessandra , Capasso Anna , Vago Riccardo , Lee Michael W , Floris Matteo , Trevisani Francesco
Published: January 2022

Renal cell carcinoma (RCC) is an increasingly common malignancy that can progress to metastatic renal cell carcinoma (mRCC) in approximately one-third of RCC patients. The 5-year survival rate for mRCC is abysmally low, and, at the present time, there are sparingly few if any effective treatments. Current surgical and pharmacological treatments can have a long-lasting impact on renal function, as well. Thus, there is a compelling unmet need to discover novel biomarkers and surveillance methods to improve patient outcomes with more targeted therapies earlier in the course of the disease. Circulating biomarkers, such as circulating tumor DNA, noncoding RNA, proteins, extracellular vesicles, or cancer cells themselves potentially represent a minimally invasive tool to fill this gap and accelerate both diagnosis and treatment. Here, we discuss the clinical relevance of different circulating biomarkers in metastatic renal cell carcinoma by clarifying their potential role as novel biomarkers of response or resistance to treatments but also by guiding clinicians in novel therapeutic approaches.