bluuebunny/crossref_metadata_2025 huggingface dataset:
Intonation Melody Therapy to Improve Language Production in Patients with Non Fluent Aphasia Disorder Post Stroke
Authors: Khamid Abdul , Nugraha Rizki Agung , Yunitri Ninik , Agustina Elis Nurhayati
Published: February 2024

Aims:  This Evidance Based Nursing Practice (EBNP) is to increase language production in patients with non-fluent aphasia post stroke.
Methods: This EBNP method uses pre and post to see an increase in language production by assessing token test scores. By calculating the number of samples G-Power version 3.1.9.7 as many as 7 respondents. Data processing using Jamovi.
Results: The analysis obtained using the t-test showed that the token test score experienced a significant change before and after the administration of intonation melody therapy (pValue <0.001), while the outcome of an increase in language production showed a significant change after administration of intonation melody therapy (pValue 0.896) with an average mean change mean 11.4 (95% CI: 6.28 -16.6) and moderate effect size (Cohen's d: 0.205).
Conclusion: provision of melodic intonation therapy is proven effective in increasing language production in patients with post stroke non fluent aphasia.
FORMULASI MASKER GEL PEEL OFF EKSTRAK BUNGA TELANG (Clitoria ternatea L.) SEBAGAI ANTIOKSIDAN ALAMI
Authors: Aulia Rahmi Azizah  , Rasidah Ananda Imroatur , Wilujeng Firdianty Lestari , Piya Nor , Yuliani Siska Dwi , Adhila Ghina , Sundu Reksi
Published: February 2024

Natural ingredients containing antioxidant compounds can be made in the form of a peel-off gel mask which can help care for facial skin. Butterfly pea flowers (Clitoria ternatea L.) contain flavonoid anthocyanin compounds which can break the chain reaction of free radicals. The aim of this research is to determine the antioxidant activity of butterfly pea flower extract and the preparation of butterfly pea peel off gel mask. The peel off gel mask formulation uses butterfly pea flower extract with a concentration of 5%. Testing for antioxidant activity uses the DPPH method. Physical characteristics assessment includes organoleptic tests, homogeneity observations, spreadability tests, viscosity tests, drying time tests, and preparation pH tests. Based on the research results, the IC50 value of telang flower extract is 43.68 ppm in the very strong category and the IC50 value of the peel-off gel mask preparation is 307.71 ppm in the inactive category. This research shows that the ethanol extract of butterfly pea flowers has potential as a natural antioxidant.
PERSPECTIVES OF THE LAYOUT OF SPECIAL CATERPILLAR TRACTOR ASSEMBLY FOR WORK ON SLOPES
Authors: GOROBEY Vasiliy , MOSKALEVICh Vadim
Published: February 2024

The results of mathematical modeling of a special caterpillar tractor of traction class 2 are presented. Calculations were carried out in Excel for various possible lengths of caterpillars with their widths equal to 200 and 300 mm, using steel and rubber-reinforced caterpillars. Based on the calculation results, graphs of the dependences of the horizontal and vertical coordinates of the center of gravity of the tractor, the angle of lateral stability, the pressure of the propellers on the soil and the ratio of the reference length of the tracks to the width of the tractor track, depending on the value of the reference length of the tracks, were plotted.
REED CANARY GRASS AND TASKS OF BREEDING NEW VARIETIES OF THIS CULTURE AT THE PRESENT STAGE
Authors: Kostenko Natalia , Kostenko Sergey , Malyuzhenets Evgenia , USOL'CEVA Elena
Published: February 2024

Reed grass (reed canary grass) – Phalaris arundinacea L., is a perennial cereal grass that naturally lives in damp places, along the banks of reservoirs or in places with close groundwater. It is found both in Eurasia and on the American continent. In Russia, this species grows everywhere, with the exception of saline areas. The species is distinguished by very vigorous growth; in terms of protein content, dwarf grass is superior to meadow timothy and orchard grass, but with the age of the grass stand, the quality of food obtained from dwarf grass sharply decreases, and the palatability of the green mass also decreases.
DE FEBRE DO RATO A BOLSONARO: CORPOS DESNUDOS VERSUS O AUTORITARISMO FARDADO
Authors: Cosmo Felipe Alexandre Moura
Published: February 2024

Com base na premissa do teórico alemão Siegfried Kracauer, na obra From Caligari to Hitler (1947), de que os filmes são capazes de refletir a mentalidade de uma nação e seus mecanismos mais ocultos, este artigo busca propor a hipótese de que o terceiro longa-metragem do diretor pernambucano Cláudio Assis, Febre do Rato (2011), a partir de sua temática visual e sua construção narrativa, antecipou as jornadas de junho de 2013, a reação violenta às manifestações por parte da polí­cia e o surgimento do bolsonarismo como força polí­tica a partir da confluência de fatores sociais, polí­ticos e econômicos. Além disso, busca fazer uma leitura do filme a partir da representação dos corpos despidos como resistência ao crescente autoritarismo das instituições e ao conservadorismo da sociedade. Além da pesquisa bibliográfica, este trabalho utiliza como metodologia a análise do filme segundo Francis Vanoye e Anne Goliot-Lété (2011). Ao longo de nossa pesquisa constatamos que Febre do Rato, ao exibir elementos que iriam marcar os anos posteriores à sua estreia, como as manifestações de cunho apartidário, a crescente militarização dos espaços urbanos e a violência policial, deixou entrever o surgimento do sentimento antipolí­tica no bojo da sociedade e a ascensão da extrema-direita nas eleições de 2018.
ARTSHOW: O CORPO E OS MATERIAIS EFÊMEROS NA ARTE PARANAENSE DO FINAL DOS ANOS 1970
Authors: Mosco Wanderson Barbieri
Published: February 2024

Este artigo é resultado de análise das ações artí­sticas realizadas durante o ARTSHOW, evento idealizado pelo artista Sérgio Moura e que ocorreu na Galeria Júlio Moreira na cidade de Curitiba entre os dias 23 de setembro e 01 de outubro de 1978. Para realizar essa análise foi feita a contextualização da presença de elementos da arte conceitual em trabalhos artí­sticos brasileiros, como o uso de materiais mais efêmeros, e da arte de performance, com a introdução do corpo como parte da obra na arte paranaense da década de 1970. Além de Sérgio Moura, vários outros artistas brasileiros participaram das ações realizadas na galeria, bem como diversos pedestres que passavam por aquele espaço e que foram chamados a contribuir. Algumas pessoas apenas atravessavam a galeria, muitas observavam as ações realizadas e outras paravam, efetivamente propondo e ajudando a compor trabalhos plásticos podendo, inclusive, ocorrer interação entre artistas e público. As ações realizadas durante esse evento enfatizaram muito mais o processo de criação artí­stica e o envolvimento do público naquele espaço com ares de ateliê do que os objetos finais resultantes das propostas executadas durante o ARTSHOW.
Ânima Trama: a dança como arqueologia sensorial
Authors: LAVAND ANA ROSANGELA COLARES
Published: February 2024

O texto que aqui se apresenta trata do processo de criação do espetáculo de dança contemporânea Ânima Trama, que é base da pesquisa de doutoramento desenvolvida no Programa de Pós-Graduação em Artes da Universidade Federal do Pará na linha de Poéticas e Processos de Atuação em Artes, nesta pesquisa, entende-se a dança como textura sensí­vel e se percorre o desejo de tecer modos sensí­veis de descrever tais acontecimentos, guiada pelo não saber me permito tecer conjecturas e desdobramentos. Este texto que é um têxtil, é também projeto evocativo de um processo de criação, de um espetáculo, mas também de uma famí­lia de mulheres e de uma criadora de arte em Belém do Pará.Bordo os acontecimentos que me conduziram a entender a constituição em meu tecido muscular e ósseo de uma mitologia corporal indí­gena na Amazônia através de uma arqueologia sensorial de minhas memórias de infância. Para tanto convoco a tecer comigo Hubert Godard e suas noções de pré-movimento e corpo mitológico e a abordagem de J. R. Pellini de arqueologia sensorial.Palavras-Chave: processo de criação; corpo mitológico e arqueologia sensorial.
PROCESSO DE CRIAÇÃO/ EDIÇÃO: UM OLHAR SOBRE A VIDEODANÇA TRÊS LUAS
Authors: Pinheiro Grégory de Souza
Published: February 2024

O presente trabalho tem como tema o processo de criação/edição em videodança. Busco aqui articular referências práticas e conceituais através do relato do caminho percorrido na criação da videodança Três Luas, coreografada e editada por mim, em meio ao período de isolamento social, devido a pandemia de COVID-19, ocorrido no ano de 2020. Além disso, visa evidenciar alguns obstáculos superados no decorrer do processo criativo. Os autores que auxiliam nas reflexões e balizam o desenvolvimento do trabalho são: Barboza (2015); Brum (2012); Caminada (1999); Cerbino e Mendonça (2012); Gonçalves (2014); Pearlman (2012); Peres (2012); Santos (2016); Shultz (2011); Através do relato do processo de criação bem como as relações traçadas paralelamente com estes autores, organizei o presente estudo em três subtítulos: Tomada um: O curso de Dança-Licenciatura e os componentes Montagem de Espetáculo; Tomada dois: Serenade ao Luar, montagem em processo; Luz, Câmera, Ação: Composição/Edição na videodança Três Luas. A escrita deste estudo faz refletir o papel do editor, o qual aparece também como coreógrafo, retrabalhando, elementos fundamentais para uma composição coreográfica em videodança, tempo e espaço.
O HORROR CORPÓREO NA PANDEMIA: UM ESTUDO SOBRE A FUGA ATRAVÉS DA FICÇÃO DO TEATRO DE HORROR
Authors: Moro Rosana
Published: February 2024

O presente artigo tem como objetivo o destrinchamento de uma performance que foi produzida como requisito parcial da avaliação para a disciplina de História da Arte VI do curso de Museologia (EMBAP/UNESPAR). Neste artigo tomo a liberdade de me expressar em quatro papéis, sendo eles o papel da personagem investigada através da performance intitulada "Babel" , o da artista-performer-produtora, o da crí­tica genética investigadora e o da museóloga em formação. Dito isso, apresento, em sua introdução, a proposta da avaliação na qual a ideia inicial foi a de produzir uma performance relacionando corpo e pandemia e então trago a correlação entre esses pontos com a minha produção num geral. Minha proposta foi trazer o horror corporificado e corporal, fazendo uso de conceitos relacionados ao teatro de horror. Como futura museóloga procurei documentar o processo a fim de pensar em uma possí­vel musealização da obra e, ao incorporar o conceito da crí­tica genética, envolvi-me na busca dos significados mais confidenciais que foram trazidos à minha mente desde o iní­cio do processo até o presente momento da escrita e leitura deste artigo, no qual, além da crí­tica genética, também trago como forma de documentar as ações vividas. Aqui me proponho a investigar mais a fundo o meu processo criativo visando uma compreensão da gênese do meu próprio processo artí­stico.
O editor nômade: o processo de edição de ví­deo enquanto prática coreográfica permeada por uma lógica da errância.
Authors: Giehl Iago , Silva Rosemeri Rocha da
Published: February 2024

Pensar a edição de ví­deo como um processo coreográfico pode ampliar os caminhos do editor e trazer novos modos de olhar para essa ferramente. A interdisciplinaridade de assuntos de diferentes campos das Artes, e aqui principalmente entre Dança e Cinema, permite novos olhares que podem transformar e ampliar a noção de alguns de seus processos. A partir de uma lógica da errância, de um editor enquanto nômade no seu processo, esse artigo busca um olhar sobre a edição enquanto modo coreográfico. Permeia também assuntos como performatividade (SETENTA, 2008) e Corpo Propositor (SILVA, 2013), que complexificam a pesquisa. O que pode mudar no seu processo e consequentemente no seu resultado, se trabalhar as imagens enquanto frases coreográficas? Como utilizar do material disponí­vel para criar seu discurso no seu fazer? Quais caminhos existem ao pensar a edição enquanto processo coreográfico? Esse artigo busca refletir e possibilitar esse olhar entrecruzado dessas práticas artí­sticas.
UM Núcleo de intergeracionalidade na arte e na educação
Authors: Corrêa Miller Sandra Maria
Published: February 2024

Este artigo inicia sua introdução com uma trajetória pessoal pavimentada pela estrutura da educação e o encanto da arte. Tem como objetivo versar sobre aspectos da pesquisa em andamento junto ao Programa de Extensão, Um-Núcleo de Pesquisa Artística em Dança oferecida pela FAP-UNESPAR, que a meu ver oferece uma interessante perspectiva intergeracional. Como integrante do Programa, apresento algumas percepções advindas das vivências e reflexões com aluno(a)s e ex-aluno(a)s, mestrand(a)os, doutorand(a)os, professores da dança e professores da música da Faculdade de Artes do Paraná. Grupo este conhecido por “Um Núcleo”, criado há 35 anos pelo colegiado do curso de dança da Unespar. Advindos da própria pesquisa-ação-reflexão alguns temas apresentados tratam da inevitável envelhescência de professores e artistas, sobre confluências e divergências veladas, não discutidas nos espaços sociais, entre as culturas do adulto envelhescente e do jovem estudante. Envolvem percepções sobre o papel das reminiscências e da formação de vínculos que dizem respeito à intra e inter intergeracionalidade. Através de dados colhidos na pesquisa teórica foi possível reconhecer que um grupo intergeracional é um espaço de compartilhamento, autoconhecimento e de inclusão participativa. Vislumbra-se pontes e muros intra e intergeracionais, porém, não há como mensurar resultados efetivos. Há o reconhecimento de que a educação e a arte podem e devem atuar como mediadoras nas trocas geracionais. Aponta ainda a possibilidade da consolidação de valores éticos e a valorização da construção do saber e do conhecimento coletivo.
Entre Tantas e Tantas, o humor conecta e vira dança
Authors: Dos Santos Gladistoni
Published: February 2024

Este artigo é fruto do desejo de especular o humor na dança a partir da articulação entre as ideias de algumas autoras, dentre elas, Verena Alberti, Henri Bergson e Abrão Slavutzky e o modo como o humor foi sendo testado no corpo a partir da trajetória e contexto de criação da Entretantas Conexão em Dança.Nessa perspectiva, o humor, em sua relação indissociável com a morte e o amor, aparece como estratégia de aproximação entre público e obra a fim de problematizarmos e alcançarmos juntos outros jeitos de experimentarmos danças. Aqui quem escreve é a pesquisadora que é mãe, mulher, artista, professora atravessada pela pandemia num país chamado Brasil e assim, o ato de escrever, também se dá em estado de susto com humor, provocado pelos desajustes e resultado das fricções entre os tempos, passado e presente e do borrar inevitável entre teoria e prática, vida e arte, contextos cotidianos e artísticos dos acadêmicos.
Effects of changing ions on the crystal design, non-covalent interactions, antimicrobial activity, and molecular docking of Cu(II) complexes with a pyridoxal-hydrazone ligand
Authors: Gatto Claudia C. , Dias Lucas M. , Paiva Clarisse A. , da Silva Izabel C. R. , Freire Daniel O. , Tormena Renata P. I. , Nascimento Érica C. M. , Martins João B. L.
Published: February 2024

The present work reports the influence of the presence of different ions (Cl−, Br−, NO3−, or SO42−) on the formation and proprieties of Cu(II) complexes with pyridoxal-benzoylhydrazone (PLBHZ). Four new complexes were successfully synthesized, [CuCl2(PLBHZ)] (1), [CuBr2(PLBHZ)] (2), [CuCl(PLBHZ)H2O]⋅NO3⋅H2O (3), and [CuSO4(PLBHZ)H2O]⋅3H2O (4), and characterized by spectroscopic and physicochemical methods. A single-crystal X-ray study reveals the Schiff base coordinated to the metal center tridentate by the ONS-donor system, resulting in distorted square pyramidal coordination geometries. Noncovalent interactions were investigated by 3D Hirshfeld surface analysis by the dnorm function, 2D fingerprint plots, and full interaction maps. The ion exchange is important in forming three-dimensional networks with π⋅⋅⋅π stacking interactions and intermolecular hydrogen bonds. The in vitro biological activity of the free ligand and metal complexes was evaluated against Gram-positive and Gram-negative bacterial strains and the free pyridoxal-hydrazone ligand showed higher activity than their Cu(II) complexes. Molecular docking was used to predict the inhibitory activity of the ligand and complexes against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli bacteria.
Obesity and acute type A aortic dissection: unraveling surgical outcomes through the lens of the upper hemisternotomy approach
Authors: Xia Lin , Liu Yu , Yang Zhonglu , Ge Yuguang , Wang Lu , Du Yejun , Jiang Hui
Published: February 2024

Background

Acute type A aortic dissection (ATAAD) is a pressing cardiovascular emergency necessitating prompt surgical intervention. Obesity, a pervasive health concern, has been identified as a significant risk factor for ATAAD, introducing unique surgical challenges that can influence postoperative outcomes. This study aimed to investigate the outcomes of ATAAD surgery across various body mass index (BMI) categories, focusing on the implications of the upper hemisternotomy (UHS) approach.

Methods

Between April 2017 and October 2023, 229 patients diagnosed with ATAAD underwent aortic arch intervention via UHS at the General Hospital of Northern Theater Command. Based on BMI (WS/T 428-2013), patients were categorized into normal weight, overweight, and obese. The primary outcomes included perioperative parameters, intraoperative details, and postoperative complications, with specific emphasis on hypoxemia, defined by the Berlin criteria as a PaO2/FiO2 ratio of ≤300 mmHg.

Results

The average age of the cohort was 50.1 ± 11.2 years with a male predominance (174 males). Preoperatively, 49.0% presented with hypoxemia, with the Obese group exhibiting a significantly elevated rate (77.9%, P < 0.001). Postoperatively, while the Normal group demonstrated a lower thoracic drainage volume 24 h post-surgery [180.0 (140.0) ml; P < 0.001], the Obese group indicated prolonged durations for mechanical ventilation and ICU stay, without statistical significance. Unlike the Normal and Overweight groups, the Obese group showed no notable changes in pre- and postoperative PaO2/FiO2 ratio. No significant difference was observed in severe postoperative complications among the groups. Further ROC curve analysis identifies a BMI cutoff of 25.5 for predicting postoperative hypoxemia, with 76.3% sensitivity and 84.4% specificity. And multivariate analysis reveals BMI and preoperative hypoxemia as independent predictors of postoperative hypoxemia.

Conclusion

Obesity, although presenting unique challenges in ATAAD interventions, does not necessarily portend adverse outcomes when managed with meticulous surgical planning and postoperative care. The study emphasizes the significance of individualized patient assessment and tailoring surgical strategies, suggesting the potential of UHS in addressing the surgical intricacies posed by obesity in ATAAD patients. Further research is warranted to consolidate these findings.
Co-creating and co-producing learning environments in adult education through the World Café method
Authors: Ravneberg Bodil Elise
Published: February 2024

Introduction

This article analyzes teachers’ efforts in preventing negative social control among newly arrived refugees at a Norwegian Adult Education Center (AEC). As the teachers realized that micro aggressive behavior hindered learning, they aimed for improving the learning environment. The World Café method (WCM) was implemented to change the learning environment in a positive way.

Methods

Data was collected by passive observation with participatory elements. This was followed up with individual and focus-group interviews on what happened during three World Café dialogues.

Results

This article uses management theories to analyse whether and how the implementation of the World Café was successful. The findings emphasize professional discretion combined with co-creation and co-production as organizational prerequisites for a positive change within the learning environment. The leaders at the AEC conducted professional discretion by employing bilingual immigrants as assistants at the AEC.

Discussion

The bilingual assistants used their multilingual resources and experiences with secondary language teaching in the planning and implementation of the World Café sessions for the newly arrived refugees. They contributed by co-creation and co-producing the WCM together with Norwegian born teachers. Their participation was an innovation at the AEC and led to a positive change in the learning environment.
Bilateral versus unilateral orchidopexy: IVF/ICSI-ET outcomes
Authors: Fan Lijuan , Shi Lin , Liu Shan , Zhang Zhou , Shi Juanzi
Published: February 2024

Introduction

Cryptorchidism is a common genital disorder. Approximately 20% of azoospermic or infertile men reported having histories of cryptorchidism. Bilateral cryptorchidism may have been more condemned than unilateral cryptorchidism. Early treatment by orchidopexy is the definitive procedure for cryptorchid patients with cryptorchidism. However, fertility potency after orchidopexy may be adversely affected and assisted reproduction techniques will be required for infertile patients.

Objective

To compare the reproductive outcomes between unilateral and bilateral orchidopexy groups.

Methods

A retrospective cohort study at a tertiary hospital, including a total of 99 infertile men who underwent orchidopexy to treat cryptorchidism and subsequently underwent their first IVF/ICSI-ET cycle. Men were grouped according to the laterality of their cryptorchidism and orchidopexy surgeries they received. Fertilization rate and live birth rate were chosen as parameters for evaluating outcomes.

Results

The sperm concentration and viability were significantly higher in unilateral orchidopexy group than in bilateral orchidopexy group (28.09 ± 27.99 vs 7.99 ± 14.68, P=0.001; 33.34 ± 22.52 vs 11.95 ± 17.85, P=0.001). Unilateral orchidopexy group showed lower demand for ICSI (66.07% vs 95.35%, P<0.001). Interestingly, both groups exhibited similar rates of fertilization, clinical pregnancy, live birth and birth defect. Boy birth ratio was lower in bilateral orchidopexy group as compared to unilateral orchidopexy group (27.27% vs 58.62%, P=0.026).

Conclusion

A history of bilateral orchidopexy surgery correlates with a worsened sperm parameter and a higher demand for ICSI as compared to patients with history of unilateral orchidopexy. However, this does not influence the final live birth rate.
The susceptibility of single nucleotide polymorphisms located within co-stimulatory pathways to systemic lupus erythematosus
Authors: Chen Ding-Ping , Lin Wei-Tzu , Hsu Fang-Ping , Yu Kuang-Hui
Published: February 2024

Introduction

Autoimmune diseases result from the loss of immune tolerance, and they exhibit complex pathogenic mechanisms that remain challenging to effectively treat. It has been reported that the altered expression levels of co-stimulatory/inhibitory molecules will affect the level of T/B cell activation and lead to the loss of immune tolerance.

Methods

In this study, we evaluated the gene polymorphisms of the ligand genes corresponding co-stimulatory system that were expressed on antigen-presenting cells (CD80, CD86, ICOSLG, and PDL1) from 60 systemic lupus erythematosus (SLE) patients and 60 healthy controls.

Results

The results showed that rs16829984 and rs57271503 of the CD80 gene and rs4143815 of the PDL1 gene were associated with SLE, in which the G-allele of rs16829984 (p=0.022), the A-allele of rs57271503 (p=0.029), and the GG and GC genotype of rs4143815 (p=0.039) may be risk polymorphisms for SLE.

Discussion

These SNPs are in the promoter and 3’UTR of the genes, so they may affect the transcription and translation activity of the genes, thereby regulating immune function and contributing to the development of SLE.
Efficacy and safety of second-line therapy by S-1 combined with sintilimab and anlotinib in pancreatic cancer patients with liver metastasis: a single-arm, phase II clinical trial
Authors: Qiu Xin , Lu Changchang , Sha Huizi , Zhu Yahui , Kong Weiwei , Tong Fan , Wang Qiaoli , Meng Fanyan , Liu Baorui , Du Juan
Published: February 2024

Background

Pancreatic adenocarcinoma carries a grim prognosis, and there are few recognized effective second-line treatment strategies. We attempted to evaluate the efficacy and safety of a combination of S-1, sintilimab, and anlotinib as a second-line treatment in pancreatic cancer patients with liver metastasis.

Methods

Pancreatic cancer patients with liver metastases were recruited. S-1 was administered orally at 25 mg/m2 bid, anlotinib was administered orally at 12 mg qd from day 1 to day 14, and sintilimab was administered intravenously at 200 mg on day 1. This method was repeated every 21 days, and the therapeutic effect was evaluated every 3 cycles. The primary outcome was the objective response rate (ORR).

Results

Overall, 23 patients were enrolled in this study of whom 19 patients had objective efficacy evaluation. The ORR was 10.5% (95% CI 0.4%–25.7%) in the evaluable population. The progression-free survival (PFS) was 3.53 (95% CI 2.50–7.50) months, and the overall survival (mOS) was 8.53 (95% CI 4.97–14.20) months. Grade 3 adverse events were 26.1%, and no grade 4 or above adverse events occurred. High-throughput sequencing was performed on the tumor tissues of 16 patients; patients with HRD-H (n = 10) had shorter PFS than those with HRD-L (n = 6) (2.43 vs. 5.45 months; P = 0.043), but there was no significant difference in OS between the two groups (4.43 vs. 9.35 months; P = 0.11).

Conclusions

This study suggests the advantage of S-1 combined with sintilimab and anlotinib in extending OS as a second-line therapy in pancreatic cancer patients with liver metastasis.

Clinical Trial Registration: ChiCTR2000030659
Pancreatic cancer tumor microenvironment is a major therapeutic barrier and target
Authors: Hartupee Conner , Nagalo Bolni Marius , Chabu Chiswili Y. , Tesfay Mulu Z. , Coleman-Barnett Joycelynn , West John T. , Moaven Omeed
Published: February 2024

Pancreatic Ductal Adenocarcinoma (PDAC) is projected to become the 2nd leading cause of cancer-related deaths in the United States. Limitations in early detection and treatment barriers contribute to the lack of substantial success in the treatment of this challenging-to-treat malignancy. Desmoplasia is the hallmark of PDAC microenvironment that creates a physical and immunologic barrier. Stromal support cells and immunomodulatory cells face aberrant signaling by pancreatic cancer cells that shifts the complex balance of proper repair mechanisms into a state of dysregulation. The product of this dysregulation is the desmoplastic environment that encases the malignant cells leading to a dense, hypoxic environment that promotes further tumorigenesis, provides innate systemic resistance, and suppresses anti-tumor immune invasion. This desmoplastic environment combined with the immunoregulatory events that allow it to persist serve as the primary focus of this review. The physical barrier and immune counterbalance in the tumor microenvironment (TME) make PDAC an immunologically cold tumor. To convert PDAC into an immunologically hot tumor, tumor microenvironment could be considered alongside the tumor cells. We discuss the complex network of microenvironment molecular and cellular composition and explore how they can be targeted to overcome immuno-therapeutic challenges.
Translational and oncologic significance of tertiary lymphoid structures in pancreatic adenocarcinoma
Authors: Gao Zachary , Azar Joseph , Zhu Huili , Williams-Perez Sophia , Kang Sung Wook , Marginean Celia , Rubinstein Mark P. , Makawita Shalini , Lee Hyun-Sung , Camp E. Ramsay
Published: February 2024

Pancreatic adenocarcinoma (PDAC) is an aggressive tumor with poor survival and limited treatment options. PDAC resistance to immunotherapeutic strategies is multifactorial, but partially owed to an immunosuppressive tumor immune microenvironment (TiME). However, the PDAC TiME is heterogeneous and harbors favorable tumor-infiltrating lymphocyte (TIL) populations. Tertiary lymphoid structures (TLS) are organized aggregates of immune cells that develop within non-lymphoid tissue under chronic inflammation in multiple contexts, including cancers. Our current understanding of their role within the PDAC TiME remains limited; TLS are complex structures with multiple anatomic features such as location, density, and maturity that may impact clinical outcomes such as survival and therapy response in PDAC. Similarly, our understanding of methods to manipulate TLS is an actively developing field of research. TLS may function as anti-tumoral immune niches that can be leveraged as a therapeutic strategy to potentiate both existing chemotherapeutic regimens and potentiate future immune-based therapeutic strategies to improve patient outcomes. This review seeks to cover anatomy, relevant features, immune effects, translational significance, and future directions of understanding TLS within the context of PDAC.
The next-generation DNA vaccine platforms and delivery systems: advances, challenges and prospects
Authors: Lu Bowen , Lim Jing Ming , Yu Boyue , Song Siyuan , Neeli Praveen , Sobhani Navid , K Pavithra , Bonam Srinivasa Reddy , Kurapati Rajendra , Zheng Junnian , Chai Dafei
Published: February 2024

Vaccines have proven effective in the treatment and prevention of numerous diseases. However, traditional attenuated and inactivated vaccines suffer from certain drawbacks such as complex preparation, limited efficacy, potential risks and others. These limitations restrict their widespread use, especially in the face of an increasingly diverse range of diseases. With the ongoing advancements in genetic engineering vaccines, DNA vaccines have emerged as a highly promising approach in the treatment of both genetic diseases and acquired diseases. While several DNA vaccines have demonstrated substantial success in animal models of diseases, certain challenges need to be addressed before application in human subjects. The primary obstacle lies in the absence of an optimal delivery system, which significantly hampers the immunogenicity of DNA vaccines. We conduct a comprehensive analysis of the current status and limitations of DNA vaccines by focusing on both viral and non-viral DNA delivery systems, as they play crucial roles in the exploration of novel DNA vaccines. We provide an evaluation of their strengths and weaknesses based on our critical assessment. Additionally, the review summarizes the most recent advancements and breakthroughs in pre-clinical and clinical studies, highlighting the need for further clinical trials in this rapidly evolving field.
MUC1 and MUC16: critical for immune modulation in cancer therapeutics
Authors: Chen Xinyi , Sandrine Ineza Karambizi , Yang Mu , Tu Jingyao , Yuan Xianglin
Published: February 2024

The Mucin (MUC) family, a range of highly glycosylated macromolecules, is ubiquitously expressed in mammalian epithelial cells. Such molecules are pivotal in establishing protective mucosal barriers, serving as defenses against pathogenic assaults. Intriguingly, the aberrant expression of specific MUC proteins, notably Mucin 1 (MUC1) and Mucin 16 (MUC16), within tumor cells, is intimately associated with oncogenesis, proliferation, and metastasis. This association involves various mechanisms, including cellular proliferation, viability, apoptosis resistance, chemotherapeutic resilience, metabolic shifts, and immune surveillance evasion. Due to their distinctive biological roles and structural features in oncology, MUC proteins have attracted considerable attention as prospective targets and biomarkers in cancer therapy. The current review offers an exhaustive exploration of the roles of MUC1 and MUC16 in the context of cancer biomarkers, elucidating their critical contributions to the mechanisms of cellular signal transduction, regulation of immune responses, and the modulation of the tumor microenvironment. Additionally, the article evaluates the latest advances in therapeutic strategies targeting these mucins, focusing on innovations in immunotherapies and targeted drugs, aiming to enhance customization and accuracy in cancer treatments.
In situ observation on two ‘elusive’ rhodaliid siphonophores (Cnidaria; Hydrozoa; Siphonophora) from the Red Sea, including a potential new species within the genus Archangelopsis
Authors: Angulo-Preckler Carlos , Steckbauer Alexandra , Lim Kah Kheng , Hissmann Karen , Pieribone Vincent , Qurban Mohammad , Duarte Carlos M.
Published: February 2024

Rhodaliids, a specific family of siphonophores inhabiting the benthic zone, have remained enigmatic due to their rarity and elusive nature. These unique organisms, primarily found in open ocean habitats, exhibit distinctive features and are characterized by complex structures. During the Red Sea Decade Expedition, two rhodaliid specimens were collected at the sea bed at water depths of 438 and 495 meters. Regardless of challenges in specimen preservation, detailed morphological analysis revealed unique characteristics. Genetic analysis, employing the 16S rRNA marker, revealed one specimen closely related to Thermopalia taraxaca, while the taxonomic traits of this specimen suggested the identification of Archangelopsis jagoa. While most of the morphological features of the second specimen were also very similar to A. jagoa, some differences in coloration suggest the discovery of either an unusual colour variant or a potential new species within this genus. Notably, this study reports the first sequencing of A. jagoa. This species demonstrated a capacity to thrive in low-oxygen environments, challenging conventional assumptions about their habitat requirements. Despite difficulties in specimen handling and genetic analysis limitations due to a lack of comprehensive data, this research sheds light on the elusive world of benthic rhodaliids.
Age and gender differences in non-motor symptoms in people with Parkinson’s disease
Authors: Maas Bart R. , Göttgens Irene , Tijsse Klasen Hermina P. S. , Kapelle Willanka M. , Radder Danique L. M. , Bloem Bastiaan R. , Post Bart , de Vries Nienke M. , Darweesh Sirwan K. L.
Published: February 2024

Background

Non-motor symptoms of Parkinson’s disease (PD) are highly prevalent and heterogenic. Previous studies aimed to gain more insight on this heterogeneity by investigating age and gender differences in non-motor symptom severity, but findings were inconsistent. Furthermore, besides examining the single effects of age and gender, the interaction between them in relation to non-motor functioning has -as far as we know- not been investigated before.

Objectives

To investigate the association of age and gender identity -as well as the interaction between age and gender identity- with non-motor symptoms and their impact on quality of life.

Methods

We combined three large and independent studies. This approach resulted in a total number of unique participants of 1,509. We used linear regression models to assess the association of age and gender identity, and their interaction, with non-motor symptoms and their impact on quality of life.

Results

Older people with PD generally had worse cognitive functioning, worse autonomic functioning and worse quality of life. Women with PD generally experienced more anxiety, worse autonomic functioning and worse quality of life compared to men with PD, whereas men with PD generally had worse cognitive functioning. In interaction analyses by age and gender identity, depressive symptoms and anxiety were disproportionally worse with increasing age in women compared to men.

Conclusion

Our findings indicate that both age and gender -as well as their interaction- are differentially associated with non-motor symptoms of PD. Both research and clinical practice should pay more attention to demographic subgroups differences and possible different treatment approaches with respect to age and gender. We showed how combining datasets is of added value in this kind of analyses and encourage others to use similar approaches.
Alterations in anthropometric, inflammatory and mental health parameters during Ramadan intermittent fasting in a group of healthy people: a prospective cohort study
Authors: Ghashang Samaneh Khosandam , Suwandi Abdulhadi , Buettner Manuela , Hamdan Imad , Grassl Guntram A. , Gutenbrunner Christoph , Nugraha Boya
Published: February 2024

Fasting has been practiced with different time span in different areas of the world and for various reasons. One of the types of fasting regimens is Ramadan intermittent fasting (RIF), which is described as intermittent dry fasting and known as the most commonly practiced form of religious fasting. Different studies have shown its effects on body composition parameters and mental health, fatigue and quality of life (QoL). Elucidating the relationship of RIF on biological parameters would also be of importance to show its mechanism. Therefore, we evaluated several biological mediators related to mental health, such as ß-nerve growth factor (ß-NGF), brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), and insulin-like growth factor-1 (IGF-1), interleukin-8 (IL-8), tumor necrosis factor-α (TNF-α), and matrix-metalloproteinase-9 (MMP-9). This study consisted of fasting (FG; n = 25) and non-fasting group (NFG; n = 25). Four different time points were assessed for FG: one week before (T1), mid (T2), last days (T3), and one week after (T4) RIF. T1 and T3 were the assessment time points for NFG. Biological mediators were determined from serum samples by using Human Magnetic Luminex and enzyme-linked immunosorbent assay. Furthermore, we then performed correlation analyses between biological mediators and our previously published clinical parameters including body composition and mental health parameters at all time points. Significant alterations were shown in FG for ß-NGF (T2vsT3, p < 0.05; T2vsT4, p < 0.05), GDNF (T1vsT4, p < 0.05; T2vsT4, p < 0.05), IL-8 (T2vsT3, p < 0.05; T3vsT4, p < 0.05), TNF-α (T1vsT3, p < 0.05; T1vsT4, p < 0.001; T2vsT4, p < 0.001), and MMP-9 (T1vsT4, p < 0.01). There were no statistically significant differences between FG and NFG in all biological mediators at T1 and T3. Correlation analysis showed that MMP-9 levels had negative correlation with body mass index (BMI) at T3. At T3 BDNF levels had negative correlation with Epworth Sleepiness Scale (ESS) as one of measured QoL parameters. ß-NGF, GDNF, TNF-α, and MMP-9 had positive correlation with some of body composition and mental health parameters. Findings demonstrate that RIF altered different biological mediators could give benefit to health. Its benefit is mediated by the alteration of biological mediators.
Validity and applicability of the global leadership initiative on malnutrition criteria in non-dialysis patients with chronic kidney disease
Authors: Huang Hui , Wang Qian , Luo Yayong , Tang Zhengchun , Liu Fang , Zhang Ruimin , Cai Guangyan , Huang Jing , Zhang Li , Zeng Li , Cao Xueying , Yang Jian , Wang Yong , Wang Keyun , Li Yaqing , Li Qihu , Chen Xiangmei , Dong Zheyi
Published: February 2024

Introduction

There are no standardized assessment criteria for selecting nutritional risk screening tools or indicators to assess reduced muscle mass (RMM) in the Global Leadership Initiative on Malnutrition (GLIM) criteria. We aimed to compare the consistency of different GLIM criteria with Subjective Global Assessment (SGA) and protein-energy wasting (PEW).

Methods

In this study, nutritional risk screening 2002 first four questions (NRS-2002-4Q), Nutritional Risk Screening 2002 (NRS-2002), Malnutrition Universal Screening Tool (MUST), and Mini-Nutritional Assessment Short-Form (MNA-SF) tools were used as the first step of nutritional risk screening for the GLIM. The RMM is expressed using different metrics. The SGA and PEW were used to diagnose patients and classify them as malnourished and non-malnourished. Kappa (κ) tests were used to compare the concordance between the SGA, PEW, and GLIM of each combination of screening tools.

Results

A total of 157 patients were included. Patients with Chronic kidney disease (CKD) stage 1–3 accounted for a large proportion (79.0%). The prevalence rates of malnutrition diagnosed using the SGA and PEW were 18.5% and 19.7%, respectively. The prevalence of GLIM-diagnosed malnutrition ranges from 5.1% to 37.6%, depending on the different screening methods for nutritional risk and the different indicators denoting RMM. The SGA was moderately consistent with the PEW (κ = 0.423, p < 0.001). The consistency among the GLIM, SGA, and PEW was generally low. Using the NRS-2002-4Q to screen for nutritional risk, GLIM had the best agreement with SGA and PEW when skeletal muscle index (SMI), fat-free mass index (FFMI), and hand grip strength (HGS) indicated a reduction in muscle mass (SGA: κ = 0.464, 95% CI 0.28–0.65; PEW: κ = 0.306, 95% CI 0.12–0.49).

Conclusion

The concordance between the GLIM criteria and the SGA and PEW depended on the screening tool used in the GLIM process. The inclusion of RMM in the GLIM framework is important. The addition of HGS could further improve the performance of the GLIM standard compared to the use of body composition measurements.
Optimization and validation of an HS-SPME/GC-MS method for determining volatile organic compounds in dry-cured ham
Authors: Babič Katja , Strojnik Lidija , Ćirić Andrija , Ogrinc Nives
Published: February 2024

The formation of volatile organic compounds (VOCs) in dry-cured ham is a result of different biochemical and enzymatic processes. Moreover, accurately quantifying these VOCs is challenging since ham is a complex matrix, which contains compounds from various chemical families and a wide range of volatilities of different molecular masses. In this study, we systematically optimized and validated an analytical method for quantifying VOCs in dry-cured ham using headspace solid phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). Optimal SPME conditions were determined through both an experimental procedure (one-factor-at-a-time) and response surface methodology (RSM), revealing that a 60-min equilibration at 70°C, a 60-min extraction at the same temperature, and a 4-min desorption time at 250°C provided the most favorable results. To enhance quantitation, twelve multiple internal standards (ISTDs) were employed to address and improve the quantitation of the 12 VOCs. Method validation covered aspects of linearity, limits of detection (LOD: 0.03–1.13 mg kg−1), limits of quantitation (LOQ: 0.09–3.41 mg kg−1), and working ranges (0.01–19.1 mg kg−1). The practical application of this optimized method was demonstrated by analyzing dry-cured ham samples (n = 4), sourced from the Slovenian market. The initial statistical evaluation indicates that different types of dry-cured hams can be differentiated (with an 83.1% of accuracy) according to their aromatic profile. However, a larger sample size would be required to provide a more comprehensive assessment.
Retrospective study on the toxicity induced by stereotactic body radiotherapy: overview of the reunion experience on prostate cancer in elderly patients
Authors: Slama Youssef , Baumont Gilles , Arcambal Angelique , Begue Mickael , Maillot Olivier , Sayah Rima , Castanet Romain , Caboche Raoul , Liberati Pedro , Slaoui Hakim , Bouaziz Medi , Borson Olivier , Nguyen Nam P. , Dutheil Fabien
Published: February 2024

Introduction

Prostate cancer is the fourth most commonly diagnosed cancer among men worldwide. Various tools are used to manage disease such as conventional radiotherapy. However, it has been demonstrated that large prostate volumes were often associated with higher rates of genitourinary and gastrointestinal toxicities. Currently, the improvements in radiotherapy technology have led to the development of stereotactic body radiotherapy, which delivers higher and much more accurate radiation doses. In order to complete literature data about short-term outcome and short-term toxic effects of stereotactic body radiotherapy, we aimed to share our experience about gastrointestinal and genitourinary toxicities associated with stereotactic body radiotherapy in prostate cancer in patients over 70 years old.

Methods

We retrospectively reviewed the medical records of elderly patients with prostate cancer treated between 2021 and 2022. The elderly patients were treated with a non-coplanar robotic stereotactic body radiotherapy platform using real-time tracking of implanted fiducials. The prostate, with or without part of the seminal vesicles, was treated with a total dose of 36.25 Gy delivered in five fractions, each fraction being administered every other day.

Results

We analyzed a total of 80 elderly patients, comprising 38 low-, 37 intermediate- and 5 high-risk patients. The median follow-up duration was 12 months. We did not observe biochemical/clinical recurrence, distant metastasis, or death. Grade 2 acute genitourinary toxicity was observed in 9 patients (11.25%) and Grade 2 acute gastrointestinal toxicity in 4 patients (5.0%). We did not observe any grade 3 or more acute or late toxicities.

Conclusion

Over the follow-up period, we noted a low frequency of gastrointestinal and genitourinary toxicities induced by stereotactic body radiotherapy in the context of prostate cancer in elderly patients. Therefore, stereotactic body radiotherapy seems to represent a promising treatment option for elderly patients, with acceptable acute toxicity.
Reporting of social determinants of health in randomized controlled trials conducted in the pediatric intensive care unit
Authors: Huang Emma , Albrecht Lisa , O’Hearn Katie , Nicolas Naisha , Armstrong Jennifer , Weinberg Maya , Menon Kusum
Published: February 2024

Introduction

The influence of social determinants of health (SDOH) on access to care and outcomes for critically ill children remains an understudied area with a paucity of high-quality data. Recent publications have highlighted the importance of incorporating SDOH considerations into research but the frequency with which this occurs in pediatric intensive care unit (PICU) research is unclear. Our objective was to determine the frequency and categories of SDOH variables reported and how these variables were defined in published PICU randomized controlled trials (RCTs).

Methods

We searched Medline, Embase, Lilacs, and Central from inception to Dec 2022. Inclusion criteria were randomized controlled trials of any intervention on children or their families in a PICU. Data related to study demographics and nine WHO SDOH categories were extracted, and descriptive statistics and qualitative data generated.

Results

586 unique RCTs were included. Studies had a median sample size of 60 patients (IQR 40-106) with 73.0% of studies including ≤100 patients and 41.1% including ≤50 patients. A total of 181 (181/586, 30.9%) studies reported ≥1 SDOH variable of which 163 (163/586, 27.8%) reported them by randomization group. The most frequently reported categories were food insecurity (100/586, 17.1%) and social inclusion and non-discrimination (73/586, 12.5%). Twenty-five of 57 studies (43.9%) investigating feeding or nutrition and 11 of 82 (13.4%) assessing mechanical ventilation reported baseline nutritional assessments. Forty-one studies investigated interventions in children with asthma or bronchiolitis of which six reported on smoking in the home (6/41, 14.6%).

Discussion

Reporting of relevant SDOH variables occurs infrequently in PICU RCTs. In addition, when available, categorizations and definitions of SDOH vary considerably between studies. Standardization of SDOH variable collection along with consistent minimal reporting requirements for PICU RCT publications is needed.