bluuebunny/crossref_metadata_2025 huggingface dataset:
Evolution of Access Network Sharing and Its Role in 5G Networks
Authors: Afraz Nima , Slyne Frank , Gill Harleen , Ruffini Marco
Published: October 2019

This paper details the evolution of access network sharing models from legacy DSL to the most recent fibre-based technology and the main challenges faced from technical and business perspectives. We first give an overview of existing access sharing models, that span physical local loop unbundling and virtual unbundled local access. We then describe different types of optical access technologies and highlight how they support network sharing. Next, we examine how the concept of SDN and network virtualization has been pivotal in enabling the idea of “true multi-tenancy”, through the use of programmability, flexible architecture and resource isolation. We give examples of recent developments of cloud central office and OLT virtualization. Finally, we provide an insight into the role that novel business models, such as blockchain and smart contract technology, could play in 5G networks. We discuss how these might evolve, to provide flexibility and dynamic operations that are needed in the data and control planes.
Stabilization of Transverse Modes for a High Finesse Near-Unstable Cavity
Authors: Liu Jianji , Liu Jiachen , Li Zhixiang , Yu Ping , Zhang Guoquan
Published: October 2019

We develop a method to lock a high-finesse near-unstable Fabry–Perot (FP) cavity (F = 7330) to a frequency stable dye laser operating at 605.78 nm using the Pound–Drever–Hall technique. The experimental results show the feasibility of locking this cavity to different transverse modes. This method links the external FP cavity to the dye laser cavity, and a 379 kHz final linewidth of the FP cavity is achieved. Such a near-unstable cavity is potentially useful for cavity-enhanced spontaneous parametric down-conversion to generate narrow-band single photon or photon pairs in different transverse modes.
Zinc Adsorption by Activated Carbon Prepared from Lignocellulosic Waste Biomass
Authors: Tuomikoski Sari , Kupila Riikka , Romar Henrik , Bergna Davide , Kangas Teija , Runtti Hanna , Lassi Ulla
Published: October 2019

Sawdust was used as a precursor for the production of biomass-based activated carbon. Carbonization and activation are single-stage processes, and steam was used as a physical activation agent at 800 °C. The adsorption capacity towards zinc was tested, and the produced activated carbon proved effective and selectively adsorbent. The effects of pH, initial concentration, adsorbent dosage, time, temperature, and regeneration cycles were tested. The adsorption capacity obtained in this study was compared favorably to that of the materials reported in the literature. Several isotherms were applied to describe the experimental results, with the Sips isotherm having the best fit. Kinetic studies showed that the adsorption follows the Elovich kinetic model.
Hormonal Regulation of Glucocorticoid Inactivation and Reactivation in αT3-1 and LβT2 Gonadotroph Cells
Authors: Michael Anthony E. , Thurston Lisa M. , Fowkes Robert C.
Published: October 2019

The regulation of reproductive function by glucocorticoids occurs at all levels of the hypothalamo-pituitary-gonadal axis. Within the pituitary, glucocorticoids have been shown to directly alter gene expression in gonadotrophs, indicating that these cell types are sensitive to regulation by the glucocorticoid receptor. Whilst the major glucocorticoid metabolising enzymes, 11β-hydroxysteroid dehydrogenase (11βHSD; HSD11B1 and HSD11B2), have been described in human pituitary adenomas, the activity of these enzymes within different pituitary cell types has not been reported. Radiometric conversion assays were performed in αT3-1, LβT2 (gonadotrophs), AtT-20 (corticotrophs) and GH3 (somatolactotrophs) anterior pituitary cell lines, using tritiated cortisol, corticosterone, cortisone or 11-dehydrocorticosterone as substrates. The net oxidation of cortisol/corticosterone and net reduction of cortisone/11-dehydrocorticosterone were significantly higher in the two gonadotroph cells lines compared with the AtT-20 and GH3 cells after 4 h. Whilst these enzyme activities remained the same in αT3-1 and LβT2 cells over a 24 h period, there was a significant increase in glucocorticoid metabolism in both AtT-20 and GH3 cells over this same period, suggesting cell-type specific activity of the 11βHSD enzyme(s). Stimulation of both gonadotroph cell lines with either 100 nM GnRH or PACAP (known physiological regulators of gonadotrophs) resulted in significantly increased 11β-dehydrogenase (11βDH) and 11-ketosteroid reductase (11KSR) activities, over both 4 and 24 h. These data reveal that gonadotroph 11βHSD enzyme activity can act to regulate local glucocorticoid availability to mediate the influence of the HPA axis on gonadotroph function.
One-step Synthesis of Silver Nanoparticles Using Saudi Arabian Desert Seasonal Plant Sisymbrium irio and Antibacterial Activity Against Multidrug-Resistant Bacterial Strains
Authors: Mickymaray Suresh
Published: October 2019

Globally, antimicrobial resistance has grown at an alarming rate. To combat the multidrug-resistant (MDR) superbugs, silver nanoparticles (Ag NPs) were synthesized using an aqueous leaf extract of seasonal desert plant Sisymbrium irio obtained from the central region of Saudi Arabia by a simple one-step procedure. The physical and chemical properties of the Ag NPs were investigated through ultraviolet visisble analysis (UV-vis), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscope (SEM), and transmission electron microscope (TEM) analysis. The UV-vis spectrum showed an absorption band at 426 nm. The XRD results showed a highly crystalline face-centered cubic structure. The surface morphology analyzed using SEM and TEM analyses showed the particle size to be in the range 24 nm to 50 nm. Various concentrations of Ag NPs were tested against MDR Pseudomonas aeruginosa and Acinetobacter baumanii that cause ventilator-associated pneumonia (VAP). American Type Culture Collection (ATCC) Escherichia coli-25922 was used as the reference control strain. The Ag NPs effectively inhibited tested pathogens, even at the lowest concentration (6.25 µg) used. The bacterial inhibitory zone ranged from 11–21 mm. In conclusion, the newly synthesized Ag NPs could be a potential alternative candidate in biomedical applications in controlling the spread of MDR pathogens.
Tandem Hydrogenation/Hydrogenolysis of Furfural to 2-Methylfuran over a Fe/Mg/O Catalyst: Structure–Activity Relationship
Authors: Lucarelli Carlo , Bonincontro Danilo , Zhang Yu , Grazia Lorenzo , Renom-Carrasco Marc , Thieuleux Chloé , Quadrelli Elsje Alessandra , Dimitratos Nikolaos , Cavani Fabrizio , Albonetti Stefania
Published: October 2019

The hydrodeoxygenation of furfural (FU) was investigated over Fe-containing MgO catalysts, on a continuous gas flow reactor, using methanol as a hydrogen donor. Catalysts were prepared either by coprecipitation or impregnation methods, with different Fe/Mg atomic ratios. The main product was 2-methylfuran (MFU), an important highly added value chemical, up to 92% selectivity. The catalyst design helped our understanding of the impact of acid/base properties and the nature of iron species in terms of catalytic performance. In particular, the addition of iron on the surface of the basic oxide led to (i) the increase of Lewis acid sites, (ii) the increase of the dehydrogenation capacity of the presented catalytic system, and (iii) to the significant enhancement of the FU conversion to MFU. FTIR studies, using methanol as the chosen probe molecule, indicated that, at the low temperature regime, the process follows the typical hydrogen transfer reduction, but at the high temperature regime, methanol dehydrogenation and methanol disproportionation were both presented, whereas iron oxide promoted methanol transfer. FTIR studies were performed using furfural and furfuryl alcohol as probe molecules. These studies indicated that furfuryl alcohol activation is the rate-determining step for methyl furan formation. Our experimental results clearly demonstrate that the nature of iron oxide is critical in the efficient hydrodeoxygenation of furfural to methyl furan and provides insights toward the rational design of catalysts toward C–O bonds’ hydrodeoxygenation in the production of fuel components.
Dimethylfumarate Inhibits Colorectal Carcinoma Cell Proliferation: Evidence for Cell Cycle Arrest, Apoptosis and Autophagy
Authors: Kaluzki Irina , Hailemariam-Jahn Tsige , Doll Monika , Kaufmann Roland , Balermpas Panagiotis , Zöller Nadja , Kippenberger Stefan , Meissner Markus
Published: October 2019

Recent studies have proven that Dimethylfumarate (DMF) has a marked anti-proliferative impact on diverse cancer entities e.g., on malignant melanoma. To explore its anti-tumorigenic potential, we examined the effects of DMF on human colon carcinoma cell lines and the underlying mechanisms of action. Human colon cancer cell line HT-29 and human colorectal carcinoma cell line T84 were treated with or without DMF. Effects of DMF on proliferation, cell cycle progression, and apoptosis were analyzed mainly by Bromodeoxyuridine (BrdU)- and Lactatdehydrogenase (LDH)-assays, caspase activation, flowcytometry, immunofluorescence, and immunoblotting. In addition, combinational treatments with radiation and chemotherapy were performed. DMF inhibits cell proliferation in both cell lines. It was shown that DMF induces a cell cycle arrest in G0/G1 phase, which is accompanied by upregulation of p21 and downregulation of cyclin D1 and Cyclin dependent kinase (CDK)4. Furthermore, upregulation of autophagy associated proteins suggests that autophagy is involved. In addition, the activation of apoptotic markers provides evidence that apoptosis is involved. Our results show that DMF supports the action of oxaliplatin in a synergetic manner and failed synergy with radiation. We demonstrated that DMF has distinct anti-tumorigenic, cell dependent effects on colon cancer cells by arresting cell cycle in G0/G1 phase as well as activating both the autophagic and apoptotic pathways and synergizes with chemotherapy.
Brush-Holder Integrated Load Sensor Prototype for SAG Grinding Mill Motor
Authors: Carrasco Miguel , Álvarez Francisco , Velazquez Ramiro , Concha Javier , Perez-Cotapos Francisco
Published: October 2019

One of the most widely used electro-mechanical systems in large-scale mining is the electric motor. This device is employed in practically every phase of production. For this reason, it needs to be inspected regularly to maintain maximum operability, thus avoiding unplanned stoppages. In order to identify potential faults, regular check-ups are performed to measure the internal parameters of the components, especially the brushes and brush-holders. Both components must be properly aligned and calibrated to avoid electric arcs to the internal insulation of the motor. Although there is an increasing effort to improve inspection tasks, most inspection procedures are manual, leading to unnecessary costs in inspection time, errors in data entry, and, in extreme cases, measurement errors. This research presents the design, development, and assessment of an integrated measurement prototype for measuring spring tension and other key parameters in brush-holders used in electric motors. It aims to provide the mining industry with a new, fully automatic inspection system that will facilitate maintenance and checking. Our development research was carried out specifically on the brush system of a SAG grinding mill motor. These machines commonly use SIEMENS motors; however, the instrument can be easily adapted to any motor by simply changing the physical dimensions of the prototype.
Scoping Review of EEG Studies in Construction Safety
Authors: Zhang Yamei , Zhang Mingyi , Fang Qun
Published: October 2019

Construction safety is critical in the success of a project. A considerable amount of effort has been placed on research and practice in order to reduce the potential risks on the construction site. Recent application of electroencephalogram (EEG) to construction research enables researchers to gain insight into construction workers’ physical and mental status during construction tasks. By summarizing existing studies that involve EEG and construction safety, the literature review aims to provide practical suggestions for future research and on-site safety management. The literature search and inclusion process included eleven eligible studies. Comprehensive analysis was conducted based on primary and secondary measures. The primary measures considered the frequency bands of EEG and the channels for detecting electrical activity of the brain. The secondary measures that were involved with physical and mental status with respect to EEG signal variations as a result of task, working hour, and work conditions. Although the field of study that combines EEG measures with construction tasks is still emerging, it is worth continuous attention in the future, as relevant findings would be of great value to the safety management and risk control in the construction industry.
Cannabis and Psychosis Through the Lens of DSM-5
Authors: Pearson Nathan T. , Berry James H.
Published: October 2019

Evidence for an association between cannabis and psychosis has been documented in literature in many forms including experimental studies, epidemiological data, and case series. The association has implications for psychotic outcomes ranging from mild to severe and occurring over minutes to years. Due to the huge variety of exposures and outcome measures reported, creating a coherent account of all the available information is difficult. A useful way to conceptualize these wide-ranging results is to consider the association between cannabis and psychosis as it occurs within the context of widely used DSM-5 diagnoses. In the present review we examine cannabis/psychosis associations as they pertain to Cannabis Intoxication, Cannabis-Induced Psychotic Disorder, and Schizophrenia. This allows for an understanding of the cannabis and psychosis association along something approaching a continuum. Cannabis intoxication becomes Cannabis-Induced Psychotic Disorder once certain severity and duration criteria are met and Cannabis-Induced Psychotic Disorder is heavily associated with future schizophrenia diagnoses.
Sexuality and Mental Health: The Need for Mutual Development and Research
Authors: Montejo Angel L.
Published: October 2019

Research in the field of sexuality has shown growing scientific development in recent years, although there’s a lack of well-trained professionals who could contribute to increasing its benefits. Sexuality continues to be a taboo with different interpretations and difficult delimitation of either normal or pathological behavior. More resources are needed for the understanding of new emerging pathologies, and to increase the research in new models of sexual behavior. All psychiatric diseases include symptoms affecting sexual life, such as impaired desire, arousal, or sexual satisfaction that need to be properly addressed. Health providers and prescribers must detect and prevent iatrogenic sexual dysfunction that can highly deteriorate a patient’s sexual life and satisfaction, leading to frequent drop-outs of medication. Approaching and researching aspects of sexual intimacy, life desires, frustrations, and fears undoubtedly constitutes the best mental health care.
Is There Any Role for Super-Extended Limphadenectomy in Advanced Gastric Cancer? Results of an Observational Study from a Western High Volume Center
Authors: Bencivenga Maria , Verlato Giuseppe , Mengardo Valentina , Scorsone Lorenzo , Sacco Michele , Torroni Lorena , Giacopuzzi Simone , de Manzoni Giovanni
Published: October 2019

Background: Although the Japan Clinical Oncology Group (JCOG) 9501 trial did not find that prophylactic D3 lymphadenectomy led to any survival advantage over D2 lymphadenectomy, it did find that the prognosis of subserosal and N0 gastric cancer patients improved. The aim of this retrospective observational study was to compare survival after D2 or D3 lymphadenectomy in different patient subgroups. Methods: The study considered all of the patients who underwent D2 or D3 lymphadenectomy at a high-volume center in Verona (Italy) between 1992 and 2011. After excluding patients with Bormann IV or neuroendocrine tumors, early gastric cancers, or non-curative resections, the analysis involved 301 R0 patients: 100 who underwent D2, and 201 who underwent D3 lymphadenectomy. Post-operative deaths and deaths due to recurrences were considered as terminal events in the survival analysis. Results: The D2 patients were significantly older than the D3 patients at baseline (69.8 ± 2.3 vs. 62.2 ± 10.7 years). The median number of retrieved nodes was 29 (interquartile range: 24.5–39) after D2, and 43 (34–52) after D3. The five-year disease-related survival rate was similar after D2 (44%, 95% confidence interval (CI) 34–54%) and D3 (41%, 34–48%) (p = 0.766). A Cox model controlling for sex, age, tumor site, Laurén histology, and T and N stages showed that the risk of cancer-related death after D3 was similar to that recorded after D2 (hazard ratio 0.97, 95% CI 0.67–1.42). There was a significant interaction between the T status and the extension of the lymphadenectomy (p = 0.012), with the prognosis being better after D2 in T2 and T4b patients, and after D3 in T3 patients. Conclusions: The findings of this study suggest that D3 lymphadenectomy is not routinely indicated for patients with advanced gastric cancer, although differences in survival after D3 across T tiers deserve further consideration.
Characterization and Potential Applications of Dog Natural Killer Cells in Cancer Immunotherapy
Authors: Gingrich Alicia A. , Modiano Jaime F. , Canter Robert J.
Published: October 2019

Natural killer (NK) cells of the innate immune system are a key focus of research within the field of immuno-oncology based on their ability to recognize and eliminate malignant cells without prior sensitization or priming. However, barriers have arisen in the effective translation of NK cells to the clinic, in part because of critical species differences between mice and humans. Companion animals, especially dogs, are valuable species for overcoming many of these barriers, as dogs develop spontaneous tumors in the setting of an intact immune system, and the genetic and epigenetic factors that underlie oncogenesis appear to be similar between dogs and humans. Here, we summarize the current state of knowledge for dog NK cells, including cell surface marker phenotype, key NK genes and genetic regulation, similarities and differences of dog NK cells to other mammals, especially human and mouse, expression of canonical inhibitory and activating receptors, ex vivo expansion techniques, and current and future clinical applications. While dog NK cells are not as well described as those in humans and mice, the knowledge of the field is increasing and clinical applications in dogs can potentially advance the field of human NK biology and therapy. Better characterization is needed to truly understand the similarities and differences of dog NK cells with mouse and human. This will allow for the canine model to speed clinical translation of NK immunotherapy studies and overcome key barriers in the optimization of NK cancer immunotherapy, including trafficking, longevity, and maximal in vivo support.
Ronald Reagan, the Modern Right, and…the Rise of the Fem-Crits
Authors: Baumgardner Paul
Published: October 2019

Activists and academics are returning to the 1980s for clues and context concerning the modern Right in the United States, oftentimes with the hope of deriving insights that can be wielded against the legal agenda of the Trump administration. This is a worthwhile historical endeavor, which must not ignore the essential position of feminist legal theorists. This article reveals the foundational role of feminist critical legal scholars, or “Fem-Crits”, to the progressive resistance against conservative legal thought during the 1980s. By highlighting the work of Fem-Crits in the academy and within the critical legal studies movement, this article identifies the Fem-Crits as a valuable source of movement inspiration and theoretical influence for leftist law professors, lawyers, and activists in the twenty-first century.
Knowledge about Disease, Medication Therapy, and Related Medication Adherence Levels among Patients with Hypertension
Authors: Gavrilova Anna , Bandere Dace , Rutkovska Ieva , Šmits Dins , Mauriņa Baiba , Poplavska Elita , Urtāne Inga
Published: October 2019

Background and Objectives: A particular problem in cardiology is poor adherence to pharmacological treatment among patients with hypertension. It is known that approximately half of these patients do not use their medications as prescribed by their doctor. Patients may choose not to follow the doctor’s recommendations and regularly do not control their blood pressure, owing to many factors. A convenient method for measuring the level of adherence is the Morisky Medication Adherence Scale, which also provides insight into possible remedies for low adherence. We investigated their therapy, knowledge about the disease and its control, and demographic differences to assess the adherence of patients with hypertension. Materials and Methods: This was a cross-sectional observational study. Data were collected through a survey of 12 pharmacies in Latvia. The study involved 187 participants with hypertension. Results: The prevalence of non-adherence was 46.20% in Latvia. The oldest patients were the most adherent (p = 0.001, β = 0.27). The higher the self-rated extent from 0 to 10, to which the patient takes their antihypertensives exactly as instructed by their physician, the higher the level of adherence (p < 0.0001, β = 0.38), where at “0”, the patient does not follow physician instructions at all, and at “10”, the patient completely follows the physician’s instructions. Non-adherent patients tend to assess their medication-taking behavior more critically than adherent patients. The longer the patient is known to suffer from hypertension, the more adherent he or she is (p = 0.014, β = 0.19). Conclusions: Medication non-adherence among patients with hypertension is high in Latvia. Further investigations are needed to better understand the reasons for this and to establish interventions for improving patient outcomes.
Dissimilar Welding and Joining of Cemented Carbides
Authors: Ma Binghui , Wang Xiaonan , Chen Chunhuan , Zhou Dongran , Xu Peiquan , Zhao Xiujuan
Published: October 2019

Cemented carbides have been widely used in aerospace, biomedical/wearable sensor, automobile, microelectronic, and other manufacturing industries owing to their superior physical and chemical properties at elevated temperatures. These superior properties, however, make it difficult to process these materials using conventional manufacturing methods. In this article, an overview of the welding and joining processes of cemented carbide and steel is given, followed by a few examples of welding processes. Cemented carbides can be successfully joined by sinter-bonding, brazing and soldering, laser beam welding, tungsten inert gas (TIG) welding, diffusion welding, friction welding, electron-beam welding, and chemical vapor deposition. An overview of the benefits and drawbacks of brazing and soldering of cemented carbide and steel is presented, including reports on joint design, processes, and selection of brazing filler metals. The laser welding of cemented carbide and steel is addressed and reviewed, including reports on gap bridging ability, the inclusion/absence of filler metals, interlayers, and laser/TIG hybrid welding. Finally, a section is devoted to explaining the main issues remaining in the welding and joining of cemented carbide, corresponding solutions, and future work required.
The Influence of Pre- and Post-Heat Treatment on Mechanical Properties and Microstructures in Friction Stir Welding of Dissimilar Age-Hardenable Aluminum Alloys
Authors: Jia Yang , Lin Sicong , Liu Jizi , Qin Yonggui , Wang Kehong
Published: October 2019

An Al-Mg-Si alloy 6061 and an Al-Zn-Mg alloy 7A52 were joined by friction stir welding successfully. Pre- and post- heat treatment were employed to improve the strength of the weld. The results show a best weld joint with the lowest hardness of 100 HV in 6061 matrix, being achieved by post-solid-solution and subsequent two-stage artificial aging for the whole weld joint of the 7A52 and 6061 solid solution. Under this condition, the weld nugget zone (WNZ) is stronger than 6061 matrix but it has lower hardness than 7A52 matrix. The hardness of WNZ is contributed by the combination of η′ and L precipitates, dynamically changes along with the ratios between the number of η′ and L precipitates. The higher the number density of η′ precipitates, the hardness of WNZ is closer to that of the 7A52 matrix. Otherwise, the higher number density of L precipitates, the hardness of WNZ is closer to that of 6061 matrix. The coexistence of η′ and L precipitates is a direct result from the mixture of 7A52 and 6061 alloys achieved by stirring. Precipitates identification and composition analysis reveal a dynamic WNZ with constituent transition in hardness and composition.
Source and Tectonic Setting of Porphyry Mo Deposits in Shulan, Jilin Province, China
Authors: Ju Nan , Zhang Sen , Kou Lin-Lin , Wang Hai-Po , Zhang Di , Gu Yu-Chao , Wu Tong
Published: October 2019

The Shulan area in Jilin Province is a part of the Lesser Xing’an–Zhangguangcai Range polymetallic ore belt, which is an important Cu–Mo ore region of northeast China. The discovery of three large Mo ore deposits (Fu’anbu, Chang’anbu, and Jidetun) highlights its potential for porphyry Mo ore deposits. Here we investigated the tectonic setting and mineralization of Mo ore deposits in the Shulan area, based on comparative study of the Fu’anbu, Chang’anbu, and Jidetun deposits. The ore-controlling structures are NE–SW- and NW–SE-trending faults. The main ore mineral in all three deposits is molybdenite. The ore bodies are all hosted in granites, have a stratiform or lenticular shape, and have strongly altered wall rocks. These observations indicate the Mo deposits in the Shulan area are typical porphyry Mo deposits. All were formed during the early Yanshanian (199.6–133.9 Ma). Biotite adamellites from the Chang’anbu deposit yield a U–Pb age of 182.10 ± 1.20 Ma. Molybdenites from the Fu’anbu and Jidetun deposits have Re–Os isochron ages of 166.9 ± 6.7 and 169.1 ± 1.8 Ma, respectively. Quartz and ore minerals were analysed for H–O and S–Pb isotopes, respectively. The results suggest the ore-forming materials were predominantly of upper-mantle origin, with secondary contributions from the lower crust. The ore-hosting granites have high concentrations of SiO2 (66.67–75.43 wt.%) and Al2O3 (12.91–16.44 wt.%), low concentrations of MgO (0.09–1.54 wt.%), and Ritman index (σ = K2O + Na2O)2/(SiO2 − 43)) ratios of 2.09–2.57. The granites are enriched in large-ion lithophile elements and depleted in high-field-strength elements, and have negative Eu anomalies. The ore-hosting rocks are geochemically similar to granites in northeastern China that were generated in a collisional orogeny. We conclude that early Yanshanian (199.6–133.9 Ma) mantle–crust-derived magmatism caused by the subduction of the Palaeo-Pacific Plate was the main source of Mo deposits in the Shulan area.
New Insights into the Reaction Paths of Hydroxyl Radicals with Purine Moieties in DNA and Double-Stranded Oligodeoxynucleotides
Authors: Chatgilialoglu Chryssostomos , Krokidis Marios G. , Masi Annalisa , Barata-Vallejo Sebastian , Ferreri Carla , Terzidis Michael A. , Szreder Tomasz , Bobrowski Krzysztof
Published: October 2019

The reaction of hydroxyl radical (HO•) with DNA produces many primary reactive species and many lesions as final products. In this study, we have examined the optical spectra of intermediate species derived from the reaction of HO• with a variety of single- and double-stranded oligodeoxynucleotides and ct-DNA in the range of 1 μs to 1 ms by pulse radiolysis using an Intensified Charged Coupled Device (ICCD) camera. Moreover, we applied our published analytical protocol based on an LC-MS/MS system with isotopomeric internal standards to enable accurate and precise measurements of purine lesion formation. In particular, the simultaneous measurement of the four purine 5′,8-cyclo-2′-deoxynucleosides (cPu) and two 8-oxo-7,8-dihydro-2′-deoxypurine (8-oxo-Pu) was obtained upon reaction of genetic material with HO• radicals generated either by γ-radiolysis or Fenton-type reactions. Our results contributed to the debate in the literature regarding absolute level of lesions, method of HO• radical generation, 5′R/5′S diastereomeric ratio in cPu, and relative abundance between cPu and 8-oxo-Pu.
Copper Ions Removal from Water using A2B3 Type Hyperbranched Poly(amidoamine) Hydrogel Particles
Authors: Choi Hojung , Eom Youngsik , Lee Sanghwa , Kim Sang Youl
Published: October 2019

Micrometer-sized hyperbranched poly(amidoamine) (hPAMAM) particles are prepared with a simple A2B3 type Aza–Michael addition reaction between aminoethylpiperazine (AEP) and methylenebisacrylamide (MBA) in an inverse suspension polymerization condition. The synthesized particles exhibited surprisingly high Cu2+ sorption capacity (0.223g/g) for a solid-type absorbent. In addition to the high sorption ability of the particle, its simple synthetic process and convenience, due to its micrometer-sized spherical shape and recyclability, make it a practical and attractive absorbent for heavy metal ion removal from aqueous solutions.
Hybrid Ionic Silver and Magnetite Microgels Nanocomposites for Efficient Removal of Methylene Blue
Authors: Atta Ayman M. , Gafer Amany K. , Al-Lohedan Hamad A. , Abdullah Mahmood M. S. , Tawfeek Ahmed M. , Ezzat Abdelrahman O.
Published: October 2019

The ionic crosslinked 2-acrylamido-2-methylpropane sulfonic acid-co-acrylic acid hydrogel, AMPS/AA and its Ag and Fe3O4 composites were synthesized using an in situ technique. The surface charge, particle sizes, morphology, and thermal stability of the prepared AMPS/AA-Ag and AMPS/AA-Fe3O4 composites were evaluated using different analytical techniques and their adsorption characteristics were evaluated to remove the methylene blue cationic dye, MB, from their aqueous solutions at optimum conditions. Also, the same monomers were used to synthesize AMPS/AA microgel and its Ag and Fe3O4 nanocomposites, which were synthesized using the same technique. The AMPS/AA-Fe3O4 nanocomposite was selected as conventional iron-supported catalyst due to the presence of both Fe(II) and Fe(III) species besides its magnetic properties that allow their easy, fast, and inexpensive separation from the aqueous solution. It was then evaluated as a heterogeneous catalyst for complete MB degradation from aqueous solution by heterogeneous Fenton oxidation. It achieved a high rate of degradation, degrading 100 mg L−1 of MB during a short time of 35 min as compared with the reported literature.
Replication Stress and Consequential Instability of the Genome and Epigenome
Authors: Tsegay Pawlos S. , Lai Yanhao , Liu Yuan
Published: October 2019

Cells must faithfully duplicate their DNA in the genome to pass their genetic information to the daughter cells. To maintain genomic stability and integrity, double-strand DNA has to be replicated in a strictly regulated manner, ensuring the accuracy of its copy number, integrity and epigenetic modifications. However, DNA is constantly under the attack of DNA damage, among which oxidative DNA damage is the one that most frequently occurs, and can alter the accuracy of DNA replication, integrity and epigenetic features, resulting in DNA replication stress and subsequent genome and epigenome instability. In this review, we summarize DNA damage-induced replication stress, the formation of DNA secondary structures, peculiar epigenetic modifications and cellular responses to the stress and their impact on the instability of the genome and epigenome mainly in eukaryotic cells.
Chemical Compositions and Mosquito Larvicidal Activities of Essential Oils from Piper Species Growing Wild in Central Vietnam
Authors: Huong Le Thi , Hung Nguyen Huy , Dai Do Ngoc , Tai Thieu Anh , Hien Vu Thi , Satyal Prabodh , Setzer William N.
Published: October 2019

Mosquitoes are the deadliest animals on earth and are the vectors of several neglected tropical diseases. Recently, essential oils have emerged as potential renewable, cost-effective, and environmentally benign alternatives to synthetic pesticides for control of mosquitoes. In this work, thirteen species of Piper were collected from different areas of central Vietnam. The essential oils were obtained by hydrodistillation and analyzed by gas chromatography–mass spectrometry. The essential oils were screened for mosquito larvicidal activity against Aedes aegypti. Four of the Piper essential oils showed outstanding larvicidal activity against Ae. aegypti, namely P. caninum, P. longum, P. montium, and P. mutabile, with LC50 and LC90 values less than 10 µg/mL. Multivariate analysis has correlated concentrations of β-caryophyllene, β-bisabolene, α-pinene, and β-pinene with mosquito larvicidal activity.
Eleutheroside B Protects against Acute Kidney Injury by Activating IGF Pathway
Authors: Zang Hongmei , Yang Qin , Li Jun
Published: October 2019

Acute kidney injury (AKI) is a common, complex, and severe clinical syndrome characterized by rapid decline in renal function, combined with tissue damage. Currently, the prevention and treatment of AKI are focused on symptomatic treatment, rather than treating the underlying causes. Therefore, there is no specific treatment to prevent renal injury except for renal dialysis. In this study, we used cisplatin-induced AKI mouse and human kidney-2 (HK-2) cell models to evaluate the renal protective effect of eleutheroside B, an active compound in traditional Chinese medicines. MTT assay was used to detect the effect of eleutheroside B on proliferation of human HK-2 cells in presence and in absence of cisplatin. Western blot and immunostaining were used to detect the protein level of kidney injury molecule-1 (KIM-1), cleaved caspase-3, receptor-interacting protein kinase (RIPK)-1, and RIPK-3. Real-time PCR was used to detect the mRNA levels of chemokines (like monocyte chemotactic protein 1, MCP-1) and pro-inflammatory cytokines including interleukin-6 (IL-6) and tumor necrosis factor (TNF-α). Flow cytometry assay was used to detect apoptosis of HK-2 cells. In vivo results showed that eleutheroside B reduced the increase in serum creatinine and blood urea nitrogen (BUN) levels in the AKI model. Periodic acid-Schiff staining and Western blot analysis of KIM-1 showed that eleutheroside B alleviated tubular cell injury. Further, eleutheroside B reduced macrophage infiltration and production of inflammatory cytokines, inhibited the activation of nuclear factor (NF)-κB, and inhibited apoptosis and programmed necrosis. The mechanism may be that eleutheroside B can activate the insulin-like growth factor (IGF) pathway and its downstream pathway by downregulating the expression of IGFBP-7, thus promoting cell proliferation. Therefore, our results suggest that eleutheroside B is a potential drug for AKI treatment.
Impact of Nitrogen Fertilizer Levels on Metabolite Profiling of the Lycium barbarum L. Fruit
Authors: Shi Zhigang , Wei Feng , Wan Ru , Li Yunxiang , Wang Yajun , An Wei , Qin Ken , Dai Guoli , Cao Youlong , Feng Jiayue
Published: October 2019

The yield and quality of goji (Lycium barbarum L.) fruit are heavily dependent on fertilizer, especially the availability of nitrogen, phosphorus, and potassium (N, P, and K, respectively). In this study, we performed a metabolomic analysis of the response of goji berry to nitrogen fertilizer levels using an Ultra Performance Liquid Chromatography–Electrospray Ionization–Tandem Mass Spectrometry (UPLC-ESI-MS/MS) method. There was no significant difference in the fruit yield or the commodity grade between N0 (42.5 g/plant), N1 (85 g/plant), and N2 (127.5 g/plant). The primary nutrients of the goji berry changed with an increasing nitrogen fertilization. Comparative metabolomic profiling of three nitrogen levels resulted in the identification of 612 metabolites, including amino acids, flavonoids, carbohydrates, organic acids, and lipids/alcohols, among others, of which 53 metabolites (lipids, fatty acids, organic acids, and phenolamides) demonstrated significant changes. These results provide new insights into the molecular mechanisms of the relationship between yield and quality of goji berry and nitrogen fertilizer.
Praziquantel-Solid Lipid Nanoparticles Produced by Supercritical Carbon Dioxide Extraction: Physicochemical Characterization, Release Profile, and Cytotoxicity
Authors: Andrade Luciana N. , Oliveira Daniele M.L. , Chaud Marco V. , Alves Thais F.R. , Nery Marcelo , da Silva Classius F. , Gonsalves Joyce K.C. , Nunes Rogéria S. , Corrêa Cristiane B. , Amaral Ricardo G. , Sanchez-Lopez Elena , Souto Eliana B. , Severino Patrícia
Published: October 2019

Solid lipid nanoparticles (SLNs) can be produced by various methods, but most of them are difficult to scale up. Supercritical fluid (SCF) is an important tool to produce micro/nanoparticles with a narrow size distribution and high encapsulation efficiency. The aim of this work was to produce cetyl palmitate SLNs using SCF to be loaded with praziquantel (PZQ) as an insoluble model drug. The mean particle size (nm), polydispersity index (PdI), zeta potential, and encapsulation efficiency (EE) were determined on the freshly prepared samples, which were also subject of Differential Scanning Calorimetry (DSC), Fourier-Transform Infrared Spectroscopy (FTIR), drug release profile, and in vitro cytotoxicity analyses. PZQ-SLN exhibited a mean size of ~25 nm, PdI ~ 0.5, zeta potential ~−28 mV, and EE 88.37%. The DSC analysis demonstrated that SCF reduced the crystallinity of cetyl palmitate and favored the loading of PZQ into the lipid matrices. No chemical interaction between the PZQ and cetyl palmitate was revealed by FTIR analysis, while the release or PZQ from SLN followed the Weibull model. PZQ-SLN showed low cytotoxicity against fibroblasts cell lines. This study demonstrates that SCF may be a suitable scale-up procedure for the production of SLN, which have shown to be an appropriate carrier for PZQ.
Electrical Characteristics and Reliability of Nitrogen-Stuffed Porous Low-k SiOCH/Mn2O3−xN/Cu Integration
Authors: Cheng Yi-Lung , Lin Yu-Lu , Lee Chih-Yen , Chen Giin-Shan , Fang Jau-Shiung
Published: October 2019

In our previous study, a novel barrier processing on a porous low-dielectric constant (low-k) film was developed: an ultrathin Mn oxide on a nitrogen-stuffed porous carbon-doped organosilica film (p-SiOCH(N)) as a barrier of the Cu film was fabricated. To form a better barrier Mn2O3−xN film, additional annealing at 450 °C was implemented. In this study, the electrical characteristics and reliability of this integrated Cu/Mn2O3−xN/p-SiOCH(N)/Si structure were investigated. The proposed Cu/Mn2O3−xN/p-SiOCH(N)/Si capacitors exhibited poor dielectric breakdown characteristics in the as-fabricated stage, although, less degradation was found after thermal stress. Moreover, its time-dependence-dielectric-breakdown electric-field acceleration factor slightly increased after thermal stress, leading to a larger dielectric lifetime in a low electric-field as compared to other metal-insulator-silicon (MIS) capacitors. Furthermore, its Cu barrier ability under electrical or thermal stress was improved. As a consequence, the proposed Cu/Mn2O3−xN/p-SiCOH(N) scheme is promising integrity for back-end-of-line interconnects.
Carbohydrate-Based Chiral Iodoarene Catalysts: A Survey through the Development of an Improved Catalyst Design
Authors: Imrich Michael R. , Biehler Linda E. , Maichle-Mössmer Cäcilia , Ziegler Thomas
Published: October 2019

Iodoarene catalysts can be applied in versatile reactions, for instance in the construction of complex chiral molecules via dearomatization of simple aromatic compounds. Recently, we reported the synthesis of the first carbohydrate-based chiral iodoarene catalysts and their application in asymmetric catalysis. Here we describe the synthesis of some new and improved catalysts. An account on how we got to the improved catalyst design, as well as the X-ray structure of one of the carbohydrate-based iodoarenes, is given.
Experimental Design Modeling of the Effect of Hexagonal Wurtzite—ZnO Synthesis Conditions on Its Characteristics and Performance as a Cationic and Anionic Adsorbent
Authors: Khalaf Mai M. , Da’na Enshirah , Al-Amer Kawther , Hessien Manal
Published: October 2019

Surface composite design was used to study the effect of the ZnO synthesis conditions on its adsorption of methyl orange (MO) and methylene blue (MB). The ZnO was prepared via hydrothermal treatment under different conditions including temperature (T), precursor concentration (C), pH, and reaction time (t). Models were built using four Design expert-11 software-based responses: the point of zero charge (pHzc), MO and MB removal efficiencies (RMO, RMB), MO and MB adsorption capacities (qMO, qMB), and hydrodynamic diameter of ZnO particles (Dh). ZnO was characterized by X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy, UV/VIS spectroscopy, thermal gravimetric analysis (TGA), and dynamic light scattering (DLS). The formation of ZnO was confirmed by the XRD, UV, and FTIR spectra. Results showed a very high efficiency for most of the samples for adsorption of MB, and more than 90% removal efficiency was achieved by 8 samples among 33 samples. For MO, more than 90% removal efficiency was achieved by 2 samples among 33 samples. Overall, 26 of 31 samples showed higher MB adsorption capacity than that of MO. RMB was found to depend only on the synthesis temperature while RMO depends on temperature, pH, and reaction time. pHzc was found to be affected by the synthesis pH only while Dh depends on the synthesis pH and precursor concentration.
Detection of Second Line Drug Resistance among Drug Resistant Mycobacterium Tuberculosis Isolates in Botswana
Authors: Mogashoa Tuelo , Melamu Pinkie , Derendinger Brigitta , Ley Serej D. , Streicher Elizabeth M. , Iketleng Thato , Mupfumi Lucy , Mokomane Margaret , Kgwaadira Botshelo , Rankgoane-Pono Goabaone , Tsholofelo Thusoyaone T. , Kasvosve Ishmael , Moyo Sikhulile , Warren Robin M. , Gaseitsiwe Simani
Published: October 2019

The emergence and transmission of multidrug resistant (MDR) and extensively drug resistant (XDR) Mycobacterium tuberculosis (M.tb) strains is a threat to global tuberculosis (TB) control. The early detection of drug resistance is critical for patient management. The aim of this study was to determine the proportion of isolates with additional second-line resistance among rifampicin and isoniazid resistant and MDR-TB isolates. A total of 66 M.tb isolates received at the National Tuberculosis Reference Laboratory between March 2012 and October 2013 with resistance to isoniazid, rifampicin or both were analyzed in this study. The genotypes of the M.tb isolates were determined by spoligotyping and second-line drug susceptibility testing was done using the Hain Genotype MTBDRsl line probe assay version 2.0. The treatment outcomes were defined according to the Botswana national and World Health Organization (WHO) guidelines. Of the 57 isolates analyzed, 33 (58%) were MDR-TB, 4 (7%) were additionally resistant to flouroquinolones and 3 (5%) were resistant to both fluoroquinolones and second-line injectable drugs. The most common fluoroquinolone resistance-conferring mutation detected was gyrA A90V. All XDR-TB cases remained smear or culture positive throughout the treatment. Our study findings indicate the importance of monitoring drug resistant TB cases to ensure rapid detection of second-line drug resistance.
Evaluation of In Vitro Solar Protection Factor (SPF), Antioxidant Activity, and Cell Viability of Mixed Vegetable Extracts from Dirmophandra mollis Benth, Ginkgo biloba L., Ruta graveolens L., and Vitis vinífera L.
Authors: Cefali Letícia Caramori , Ataide Janaina Artem , Fernandes Ana Rita , Sanchez-Lopez Elena , Sousa Ilza Maria de Oliveira , Figueiredo Mariana Cecchetto , Ruiz Ana Lucia Tasca Gois , Foglio Mary Ann , Mazzola Priscila Gava , Souto Eliana Barbosa
Published: October 2019

The aim of this study was to validate a HPLC method for the assay of flavonoids in extracts obtained from natural sources, i.e., from Dirmophandra mollis Benth, Ginkgo biloba L., Ruta graveolens L., and Vitis vinífera L. The potential sun protecting effect, antioxidant activity, and cell viability of the extracts were also determined. Individual extracts (obtained from each individual species) and a mixed extract (containing the four extracts) were analyzed by the validated HPLC method for the identification of flavonoids and quantification of rutin and quercetin. An in vitro cell viability study was carried out using the neutral red method. The in vitro sun protection factor was determined by spectral transmittance and in vitro antioxidant efficacy was evaluated against DPPH, ABTS, and AAPH radicals. The HPLC method used for the identification and quantification of flavonoids in extracts exhibited linearity, precision, accuracy, and robustness. Detection and quantification limits were, respectively, 2.881 ± 0.9 μg·mL−1 and 0.864 ± 0.9 μg·mL−1 for quercetin, and 30.09 ± 1 μg·mL−1 and 9.027 ± 1.1 μg·mL−1 for rutin. All extracts did not affect cell viability at the evaluated concentration range and exhibited a sun protection effect and antioxidant activity. Among the evaluated extracts, Ginkgo biloba L. and the mixed extract depicted the most expressive antioxidant activity. The mixed extract exhibited sunscreen protection against ultraviolet A (UVA) and ultraviolet B (UVB) and a critical wavelength of 372.7 ± 0.1. Our results translate the enhanced flavonoids’ composition of the mixed extract, which may be a potential alternative over sunscreens and antioxidants in pharmaceutic/cosmetic formulations.
Retailing, Consumers, and Territory: Trends of an Incipient Circular Model
Authors: De-Juan-Vigaray María D. , Seguí Ana I. Espinosa
Published: October 2019

The aim of this theoretical research is to analyze the state of retail distribution nowadays, reviewing the dynamics of action that contribute to the move from a linear to an incipient circular retail model. The framework is based on the Retail Wheel Spins Theory and the Retail Life Cycle (RLC), with an extra review of Bauman’s liquid metaphor. We consider two questions. Firstly, are offline retailers ready to disappear as online commerce and digital marketing aggressively break into the retail industry? Secondly, could commercial spaces (in the fifth stage in the evolution of retail and territory) be in the decline stage in the RLC in the near future or can a circular connection take place? Thus, a desk research methodology based on secondary documentary material and sources issued leads to an interpretive analysis that reveals ten trends (e.g., solid retail vs. liquid retail; glocal retail; food sovereignty) and a wide diversity of changes that could involve offline stores recovering territory and entering a circular phase. Our findings suggest that digitalized physical stores are flourishing and our reflections augur changes in pace and the closure of the linear business cycle to recover territory, the city, its local market, and its symbolism, as well as a liquid business steeped in omnichannel formats developing an incipient circular movement. Conclusions indicate that it is possible to perceive a timid change back to territory and retail spaces which, along with phygitalization, will coexist with the digital world.