bluuebunny/crossref_metadata_2025 huggingface dataset:
Hydroxylpropyl-β-cyclodextrin as Potential Excipient to Prevent Stress-Induced Aggregation in Liquid Protein Formulations
Authors: Stolzke Tanja , Krieg Franziska , Peng Tao , Zhang Hailong , Häusler Olaf , Brandenbusch Christoph
Published: August 2022

Due to the growing demand for patient-friendly subcutaneous dosage forms, the ability to increasing protein solubility and stability in formulations to deliver on the required high protein concentrations is crucial. A common approach to ensure protein solubility and stability in high concentration protein formulations is the addition of excipients such as sugars, amino acids, surfactants, approved by the Food and Drug Administration. In a best-case scenario, these excipients fulfil multiple demands simultaneously, such as increasing long-term stability of the formulation, reducing protein adsorption on surfaces/interfaces, and stabilizing the protein against thermal or mechanical stress. 2-Hydroxylpropyl-β-cyclodextrin (derivative of β-cyclodextrin) holds this potential, but has not yet been sufficiently investigated for use in protein formulations. Within this work, we have systematically investigated the relevant molecular interactions to identify the potential of Kleptose®HPB (2-hydroxylpropyl-β-cyclodextrin from Roquette Freres, Lestrem, France) as “multirole” excipient within liquid protein formulations. Based on our results three factors determine the influence of Kleptose®HPB on protein formulation stability: (1) concentration of Kleptose®HPB, (2) protein type and protein concentration, and (3) quality of the protein formulation. Our results not only contribute to the understanding of the relevant interactions but also enable the target-oriented use of Kleptose®HPB within formulation design.
Natural Polyphenols as SERCA Activators: Role in the Endoplasmic Reticulum Stress-Related Diseases
Authors: Viskupicova Jana , Rezbarikova Petronela
Published: August 2022

Sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) is a key protein responsible for transporting Ca2+ ions from the cytosol into the lumen of the sarco/endoplasmic reticulum (SR/ER), thus maintaining Ca2+ homeostasis within cells. Accumulating evidence suggests that impaired SERCA function is associated with disruption of intracellular Ca2+ homeostasis and induction of ER stress, leading to different chronic pathological conditions. Therefore, appropriate strategies to control Ca2+ homeostasis via modulation of either SERCA pump activity/expression or relevant signaling pathways may represent a useful approach to combat pathological states associated with ER stress. Natural dietary polyphenolic compounds, such as resveratrol, gingerol, ellagic acid, luteolin, or green tea polyphenols, with a number of health-promoting properties, have been described either to increase SERCA activity/expression directly or to affect Ca2+ signaling pathways. In this review, potential Ca2+-mediated effects of the most studied polyphenols on SERCA pumps or related Ca2+ signaling pathways are summarized, and relevant mechanisms of their action on Ca2+ regulation with respect to various ER stress-related states are depicted. All data were collected using scientific search tools (i.e., Science Direct, PubMed, Scopus, and Google Scholar).
Effects of Applied Magnetic Field on the Optical Properties and Binding Energies Spherical GaAs Quantum Dot with Donor Impurity
Authors: Edet Collins Okon , Al Emre Bahadir , Ungan Fatih , Ali Norshamsuri , Rusli Nursalasawati , Aljunid Syed Alwee , Endut Rosdisham , Asjad Muhammad
Published: August 2022

The screened modified Kratzer potential (SMKP) model is utilized to scrutinize the impacts of an applied magnetic field (MF) on the binding energies and linear and nonlinear optical properties spherical GaAs quantum dot with donor impurity (DI). To accomplish this goal, we have used the diagonalization method to numerically solve the Schrödinger equation under the effective mass approximation for obtaining the electron energy levels and related electronic wave functions. The expressions used for evaluating linear, third-order nonlinear, and total optical absorption coefficients and relative refractive index changes were previously derived within the compact density matrix method. It has been shown here that the MF and DI impacts the characteristics of the absorption coefficients and the refractive index changes. This study’s results will find application in optoelectronics and related areas.
High Sensitivity SERS Substrate of a Few Nanometers Single-Layer Silver Thickness Fabricated by DC Magnetron Sputtering Technology
Authors: Wu Hsing-Yu , Lin Hung-Chun , Hung Guan-Yi , Tu Chi-Shun , Liu Ting-Yu , Hong Chung-Hung , Yu Guoyu , Hsu Jin-Cherng
Published: August 2022

Surface-enhanced Raman spectroscopy (SERS) is commonly used for super-selective analysis through nanostructured silver layers in the environment, food quality, biomedicine, and materials science. To fabricate a high-sensitivity but a more accessible device of SERS, DC magnetron sputtering technology was used to realize high sensitivity, low cost, a stable deposition rate, and rapid mass production. This study investigated various thicknesses of a silver film ranging from 3.0 to 12.1 nm by field emission scanning electron microscope, X-ray diffraction, and X-ray photoelectron spectroscopy. In the rhodamine 6G (R6G) testing irradiated by a He-Ne laser beam, the analytical enhancement factor (AEF) of 9.35 × 108, the limit of detection (LOD) of 10−8 M, and the relative standard deviation (RSD) of 1.61% were better than the other SERS substrates fabricated by the same DC sputtering process because the results showed that the 6 nm thickness silver layer had the highest sensitivity, stability, and lifetime. The paraquat and acetylcholine analytes were further investigated and high sensitivity was also achievable. The proposed SERS samples were evaluated and stored in a low humidity environment for up to forty weeks, and no spectrum attenuation could be detected. Soon, the proposed technology to fabricate high sensitivity, repeatability, and robust SERS substrate will be an optimized process technology in multiple applications.
Preparation and Characterization of an Electrospun Whey Protein/Polycaprolactone Nanofiber Membrane for Chromium Removal from Water
Authors: Ramírez-Rodríguez Laura Cristina , Quintanilla-Carvajal María Ximena , Mendoza-Castillo Didilia Ileana , Bonilla-Petriciolet Adrián , Jiménez-Junca Carlos
Published: August 2022

Chromium pollution represents a worldwide concern due to its high toxicity and bioaccumulation in organisms and ecosystems. An interesting material to remove metal ions from water is a whey-protein-based material elaborated by electrospinning, which is an emerging method to produce adsorbent membranes with diverse applications. The aim of this study was to prepare an adsorbent membrane of whey protein isolate (WPI) and polycaprolactone (PCL) by electrospinning to remove chromium ions from water. The adsorbent membrane was synthesized by a central composed design denaturing WPI using 2-Mercaptoethanol and mixing it with PCL to produce electrospun nanofibers. The adsorbent membrane was characterized by denaturation, Scanning Electron Microscope, Fourier-Transform Infrared Spectroscopy, Contact Angle, Thermogravimetric Analysis, and X-ray Photoelectron Spectrometry. The adsorption properties of this membrane were assessed in the removal of chromium. The removal performance of the membrane was enhanced by an increase in temperature showing an endothermic adsorption process. The adsorption process of chromium ions onto the nanofiber membrane followed the Sips adsorption isotherm, while the adsorption kinetics followed a pseudo-second kinetics where the maximum adsorption capacity was 31.0 mg/g at 30 °C and pH 2. This work provides a novel method to fabricate a hybrid membrane with amyloid-type fibrils of WPI and PCL, which is a promising adsorbent to remove heavy metal ions from water.
U-Shaped Relation of Dietary Thiamine Intake and New-Onset Hypertension
Authors: Zhang Yuanyuan , Zhang Yanjun , Yang Sisi , Ye Ziliang , Wu Qimeng , Liu Mengyi , Zhou Chun , He Panpan , Jiang Jianping , Liang Min , Wang Guobao , Hou Fanfan , Liu Chengzhang , Qin Xianhui
Published: August 2022

Background: To examine the relation of dietary thiamine intake with risk of new-onset hypertension in the general adults. Methods: A total of 12,177 participants without hypertension at baseline from China Health and Nutrition Survey (CHNS) were included. The study outcome was new-onset hypertension, which was defined as a systolic blood pressure ≥140 mm Hg or a diastolic blood pressure ≥90 mm Hg or under antihypertensive treatment or diagnosed by physician during the follow-up. Results: A total of 4269 participants occurred new-onset hypertension over a median follow-up of 6.1 years. Overall, there was a U-shaped relation (p for nonlinearity <0.001) of dietary thiamine intake with new-onset hypertension, with an inflection point at 0.93 mg/day. Accordingly, in the threshold effect analysis, there was an inverse association between dietary thiamine intake (per SD increment: HR, 0.62; 95% CI: 0.53, 0.72) and new-onset hypertension in participants with dietary thiamine intake <0.93 mg/day, and a positive association between dietary thiamine intake (per SD increment: HR, 1.38; 95% CI: 1.32, 1.44) and new-onset hypertension in those with dietary thiamine intake ≥0.93 mg/day. Conclusion: The association between dietary thiamine intake and the risk of new-onset hypertension followed a U-shaped relation in the general Chinese population, with an inflection point at 0.93 mg/day of dietary thiamine intake.
Association of Eating Behavior, Nutritional Risk, and Frailty with Sarcopenia in Taiwanese Rural Community-Dwelling Elders: A Cross-Sectional Study
Authors: Kuo Ya-Wen , Chen Chu-Wei , Zhang Jia-Yu , Lee Jiann-Der
Published: August 2022

This cross-sectional study assessed the association of eating behavior, nutritional risk, and frailty with sarcopenia in 208 community-dwelling individuals aged ≥65 years who were recruited from random rural community care centers in Chiayi County, Taiwan. The participants’ eating behavior was categorized into six categories. The gait speed (GS), grip strength, and appendicular skeletal muscle mass (ASM) were assessed based on these three parameters, which revealed that 50.9% of the participants had sarcopenia. In an adjusted model, water intake (odds ratio (OR) = 0.99, p = 0.044), dairy product intake (OR = 0.42, p = 0.049), body mass index (BMI) (OR = 0.77, p = 0.019), and marital status with widowed (OR = 0.31, p = 0.005) were significantly associated with sarcopenia. After eight steps of eliminating the least significant independent variable, age (p = 0.002), sex (p = 0.000), marital status with widowed (p = 0.001), water intake (p < 0.018), dairy product intake (p < 0.019), and BMI (p = 0.005) were found to be indispensable predictors of sarcopenia. The logistic regression model with these six indispensable variables had a predictive value of 75.8%. Longitudinal analyses are warranted to examine whether eating behavior is a risk factor for sarcopenia onset.
Development of a Tool for Determining the Equivalence of Nutritional Supplements to Diabetic Food Exchanges
Authors: Detopoulou Paraskevi , Panoutsopoulos Georgios , Kalonarchi Garifallia , Alexatou Olga , Petropoulou Georgia , Papamikos Vasilios
Published: August 2022

Malnutrition is a prevalent issue in hospitals, nursing homes and the community setting. Nutritional products can be used by dietitians to supplement people’s diet by adding energy, macronutrients and other constituents. The aim of the present work was (i) to create a database of nutritional products with information on their energy and macronutrient content, (ii) to estimate the food exchanges of each product and assist in diet plan development for malnourished persons and (iii) to provide a tool for calculation of food exchanges of newly developed products not included in the database. We searched the web for nutritional supplements, and an electronic database with 461 products was generated with data regarding the contained energy and macronutrients of each entry. The following companies were included: Abbott Nutrition, Nestle Nutrition, Nutricia North America, Nutricia Global, Nutricia Europe & Middle East, Axcan Pharma Inc., Kate Farms, Global Health, High Protein, NutriMedical BV, Hormel Health Labs, Hormel Health Labs/Diamond Crystal Brands, Lyons Magnus, Mead Johnson, Medical Nutrition USA Inc., Medtrition, Nutritional Designs Inc., Nutrisens, Humana (Germany), and Vitaflo USA. The created database facilitates product comparisons and categorization into several groups according to energy and protein content. In addition, a tool was created to determine food exchanges for each supplement per serving and/or food exchanges for newly developed products by simply inserting their macronutrient content. The developed tool can facilitate dietitians in comparing products and incorporating them into diet plans, if needed. Such tools may thus serve clinical practice, may be used in dietary or other smart applications and can familiarize dietitians with the digital epoch.
Short- and Long-Term Effects of Suboptimal Selenium Intake and Developmental Lead Exposure on Behavior and Hippocampal Glutamate Receptors in a Rat Model
Authors: Tartaglione Anna Maria , Serafini Melania Maria , Ferraris Francesca , Raggi Andrea , Mirabello Annalisa , Di Benedetto Rita , Ricceri Laura , Midali Miriam , Cubadda Francesco , Minghetti Luisa , Viviani Barbara , Calamandrei Gemma
Published: August 2022

Selenium (Se) is an essential trace element required for normal development as well as to counteract the adverse effects of environmental stressors. Conditions of low Se intake are present in some European countries. Our aim was to investigate the short- and long-term effects of early-life low Se supply on behavior and synaptic plasticity with a focus on the hippocampus, considering both suboptimal Se intake per se and its interaction with developmental exposure to lead (Pb). We established an animal model of Se restriction and low Pb exposure; female rats fed with an optimal (0.15 mg/kg) or suboptimal (0.04 mg/kg) Se diet were exposed from one month pre-mating until the end of lactation to 12.5 µg/mL Pb via drinking water. In rat offspring, the assessment of motor, emotional, and cognitive endpoints at different life stages were complemented by the evaluation of the expression and synaptic distribution of NMDA and AMPA receptor subunits at post-natal day (PND) 23 and 70 in the hippocampus. Suboptimal Se intake delayed the achievement of developmental milestones and induced early and long-term alterations in motor and emotional abilities. Behavioral alterations were mirrored by a drop in the expression of the majority of NMDA and AMPA receptor subunits analyzed at PND 23. The suboptimal Se status co-occurring with Pb exposure induced a transient body weight increase and persistent anxiety-like behavior. From the molecular point of view, we observed hippocampal alterations in NMDA (Glun2B and GluN1) and AMPA receptor subunit trafficking to the post-synapse in male rats only. Our study provides evidence of potential Se interactions with Pb in the developing brain.
Neuroprotective Natural Products’ Regulatory Effects on Depression via Gut–Brain Axis Targeting Tryptophan
Authors: Liaqat Humna , Parveen Amna , Kim Sun Yeou
Published: August 2022

L-tryptophan (Trp) contributes to regulating bilateral communication of the gut–brain axis. It undergoes three major metabolic pathways, which lead to formation of kynurenine, serotonin (5-HT), and indole derivatives (under the control of the microbiota). Metabolites from the principal Trp pathway, kynurenic acid and quinolinic acid, exhibit neuroprotective activity, while picolinic acid exhibits antioxidant activity, and 5-HT modulates appetite, sleep cycle, and pain. Abnormality in Trp plays crucial roles in diseases, including depression, colitis, ulcer, and gut microbiota-related dysfunctions. To address these diseases, the use of natural products could be a favorable alternative because they are a rich source of compounds that can modulate the activity of Trp and combat various diseases through modulating different signaling pathways, including the gut microbiota, kynurenine pathway, and serotonin pathway. Alterations in the signaling cascade pathways via different phytochemicals may help us explore the deep relationships of the gut–brain axis to study neuroprotection. This review highlights the roles of natural products and their metabolites targeting Trp in different diseases. Additionally, the role of Trp metabolites in the regulation of neuroprotective and gastroprotective activities is discussed. This study compiles the literature on novel, potent neuroprotective agents and their action mechanisms in the gut–brain axis and proposes prospective future studies to identify more pharmaceuticals based on signaling pathways targeting Trp.
The Effect of a Multifaceted Intervention on Dietary Quality in Schoolchildren and the Mediating Effect of Dietary Quality between Intervention and Changes in Adiposity Indicators: A Cluster Randomized Controlled Trial
Authors: Lyu Jin-Lang , Liu Zheng , Zhou Shuang , Feng Xiang-Xian , Lin Yi , Gao Ai-Yu , Zhang Fang , Li Li , Hebestreit Antje , Wang Hai-Jun
Published: August 2022

Some studies have found associations between dietary quality and obesity and their concurrent changes were observed in a few interventions. The present study aimed to assess the effect of a multifaceted intervention for childhood obesity on dietary quality and examine the mediating effect of dietary quality between the intervention and changes in adiposity indicators. Based on the social ecological model, the cluster randomized controlled trial included five components (three targeted children and two targeted their environment). In total, 1176 children from three cities in China participated in a baseline (2018) and end-of-trial (2019) examination, including 605 children in the intervention group and 571 in the control group. Self-reported behavior and anthropometric measures were collected at both time points. The Diet Balance Index Revision (DBI-07) was calculated to assess dietary quality. Generalized linear mixed models were used to estimate the intervention effect on dietary quality and its mediating effects were examined. Compared to the controls, the proportion of sugar-sweetened beverage (SSB) intake (OR = 0.27, p < 0.001, corrected p < 0.001) decreased in the intervention group. Higher bound scores (HBS) of the DBI-07 indicating over-intake decreased in the intervention group compared to the controls (mean difference = −1.52, p = 0.005, corrected p = 0.015). Changes in the HBS partially mediated the associations between the intervention and changes in body mass index, waist circumference, and body fat percentage. Future intervention should promote knowledge, attitudes, and behaviors related to dietary quality.
Hypoxia Effects on Trypanosoma cruzi Epimastigotes Proliferation, Differentiation, and Energy Metabolism
Authors: Saraiva Francis M. S. , Cosentino-Gomes Daniela , Inacio Job D. F. , Almeida-Amaral Elmo E. , Louzada-Neto Orlando , Rossini Ana , Nogueira Natália P. , Meyer-Fernandes José R. , Paes Marcia C.
Published: August 2022

Trypanosoma cruzi, the causative agent of Chagas disease, faces changes in redox status and nutritional availability during its life cycle. However, the influence of oxygen fluctuation upon the biology of T. cruzi is unclear. The present work investigated the response of T. cruzi epimastigotes to hypoxia. The parasites showed an adaptation to the hypoxic condition, presenting an increase in proliferation and a reduction in metacyclogenesis. Additionally, parasites cultured in hypoxia produced more reactive oxygen species (ROS) compared to parasites cultured in normoxia. The analyses of the mitochondrial physiology demonstrated that hypoxic condition induced a decrease in both oxidative phosphorylation and mitochondrial membrane potential (ΔΨm) in epimastigotes. In spite of that, ATP levels of parasites cultivated in hypoxia increased. The hypoxic condition also increased the expression of the hexokinase and NADH fumarate reductase genes and reduced NAD(P)H, suggesting that this increase in ATP levels of hypoxia-challenged parasites was a consequence of increased glycolysis and fermentation pathways. Taken together, our results suggest that decreased oxygen levels trigger a shift in the bioenergetic metabolism of T. cruzi epimastigotes, favoring ROS production and fermentation to sustain ATP production, allowing the parasite to survive and proliferate in the insect vector.
Matrix Tablets Based on Chitosan–Carrageenan Polyelectrolyte Complex: Unique Matrices for Drug Targeting in the Intestine
Authors: Komersová Alena , Svoboda Roman , Skalická Barbora , Bartoš Martin , Šnejdrová Eva , Mužíková Jitka , Matzick Kevin
Published: August 2022

The present study focused on the more detailed characterization of chitosan–carrageenan-based matrix tablets with respect to their potential utilization for drug targeting in the intestine. The study systematically dealt with the particular stages of the dissolution process, as well as with different views of the physico-chemical processes involved in these stages. The initial swelling of the tablets in the acidic medium based on the combined microscopy–calorimetry point of view, the pH-induced differences in the erosion and swelling of the tested tablets, and the morphological characterization of the tablets are discussed. The dissolution kinetics correlated with the rheological properties and mucoadhesive behavior of the tablets are also reported, and, correspondingly, the formulations with suitable properties were identified. It was confirmed that the formation of the chitosan–carrageenan polyelectrolyte complex may be an elegant and beneficial alternative solution for the drug targeting to the intestine by the matrix tablet.
Screening of Mineralogenic and Osteogenic Compounds in Zebrafish—Tools to Improve Assay Throughput and Data Accuracy
Authors: Rosa Joana T. , Tarasco Marco , Gavaia Paulo J. , Cancela M. Leonor , Laizé Vincent
Published: August 2022

Bone disorders affect millions of people worldwide and treatments currently available often produce undesirable secondary effects or have limited efficacy. It is therefore of the utmost interest for patients to develop more efficient drugs with reduced off-target activities. In the long process of drug development, screening and preclinical validation have recently gained momentum with the increased use of zebrafish as a model organism to study pathological processes related to human bone disorders, and the development of zebrafish high-throughput screening assays to identify bone anabolic compounds. In this review, we provided a comprehensive overview of the literature on zebrafish bone-related assays and evaluated their performance towards an integration into screening pipelines for the discovery of mineralogenic/osteogenic compounds. Tools available to standardize fish housing and feeding procedures, synchronize embryo production, and automatize specimen sorting and image acquisition/analysis toward faster and more accurate screening outputs were also presented.
Increased PDT Efficacy When Associated with Nitroglycerin: A Study on Retinoblastoma Xenografted on Mice
Authors: Thomas Carole D. , Lupu Mihaela , Poyer Florent , Maillard Philippe , Mispelter Joël
Published: August 2022

Purposes: The aim of the study was to assess the efficacy of a treatment protocol that combines photodynamic therapy (PDT) and nitroglycerin (NG) on human retinoblastoma tumors xenografted on mice. We aimed to increase the PDT efficiency (in our least treatment-responsive retinoblastoma line) with better PS delivery to the tumor generated by NG, which is known to dilate vessels and enhance the permeability and retention of macromolecules in solid tumors. Methods: In vivo follow-up of the therapeutic effects was performed by sodium MRI, which directly monitors variations in sodium concentrations non-invasively and can be used to track the tumor response to therapy. NG ointment was applied one hour before PDT. The PDT protocol involves double-tumor targeting, i.e., cellular and vascular. The first PS dose was injected followed by a second one, separated by a 3 h interval. The timelapse allowed the PS molecules to penetrate tumor cells. Ten minutes after the second dose, the PS was red-light-activated. Results: In this study, we observed that the PDT effect was enhanced by applying nitroglycerin ointment to the tumor-bearing animal’s skin. PDT initiates the bystander effect on retinoblastomas, and NG increases this effect by increasing the intratumoral concentration of PS, which induces a higher production of ROS in the illuminated region and thus increases the propagation of the cell death signal deeper into the tumor (bystander effect).
Full-Resolution Light-Field Camera via Fourier Dual Photography
Authors: Hu Yuhang , Yao Manhong , Huang Zhuobin , Peng Junzheng , Zhang Zibang , Zhong Jingang
Published: August 2022

Conventional light-field cameras with a micro-lens array suffer from resolution trade-off and shallow depth of field. Here we develop a full-resolution light-field camera based on dual photography. We extend the principle of dual photography from real space to Fourier space for obtaining two-dimensional (2D) angular information of the light-field. It uses a spatial light modulator at the image plane as a virtual 2D detector to record the 2D spatial distribution of the image, and a real 2D detector at the Fourier plane of the image to record the angles of the light rays. The Fourier-spectrum signals recorded by each pixel of the real 2D detector can be used to reconstruct a perspective image through single-pixel imaging. Based on the perspective images reconstructed by different pixels, we experimentally demonstrated that the camera can digitally refocus on objects at different depths. The camera can achieve light-field imaging with full resolution and provide an extreme depth of field. The method provides a new idea for developing full-resolution light-field cameras.
Customer Edge Downstream Detection for Automatic Restoration Scheme in FTTH-PON Distribution Network
Authors: Ab-Rahman Mohammad Syuhaimi , Manaf Zulhedry Abd. , Kaharudin Iszan Hana , Hwang I-Shyan
Published: August 2022

Fiber-to-the-home (FTTH) networks are seen as the most future-proof technology to offer increasing bandwidth to customers. By utilizing passive optical network (PON) technology, they provide flexibility and capability to carry higher bandwidths as compared to the legacy copper-based access network. Optical performance monitoring could potentially enable higher stability, reconfigurability, and flexibility in a self-managed optical network. This paper will describe the specific fiber impairments that affect the quality of service for fiber-to-the-home networks. The impairment needs to be monitored and restored. The proposed solution will utilize a photodiode and optical switch as the main components, can easily be integrated with the ONU through the optical interface, and will be referred to as ‘PROMO’. With this scheme, the protection and restoration mechanisms are archived through the detection and availability of the downstream signal from the OLT. The results show that the received power, BER values, and maximum Q factor are acceptable for both simulation and experimental conditions in the case of normal and protection conditions.
Rhizoboxes as Rapid Tools for the Study of Root Systems of Prunus Seedlings
Authors: Lesmes-Vesga Ricardo A. , Cano Liliana M. , Ritenour Mark A. , Sarkhosh Ali , Chaparro José X. , Rossi Lorenzo
Published: August 2022

Rootstocks are fundamental for peach production, and their architectural root traits determine their performance. Root-system architecture (RSA) analysis is one of the key factors involved in rootstock selection. However, there are few RSA studies on Prunus spp., mostly due to the tedious and time-consuming labor of measuring below-ground roots. A root-phenotyping experiment was developed to analyze the RSA of seedlings from ‘Okinawa’ and ‘Guardian’™ peach rootstocks. The seedlings were established in rhizoboxes and their root systems scanned and architecturally analyzed. The root-system depth:width ratio (D:W) throughout the experiment, as well as the root morphological parameters, the depth rooting parameters, and the root angular spread were estimated. The ‘Okinawa’ exhibited greater root morphological traits, as well as the other parameters, confirming the relevance of the spatial disposition and growth pattern of the root system.
Seasonal Variation of Aromatic Plants under Cultivation Conditions
Authors: Stefanakis Michalis K. , Papaioannou Charikleia , Lianopoulou Vaia , Philotheou-Panou Eleni , Giannakoula Anastasia E. , Lazari Diamanto M.
Published: August 2022

In this study, five plant species, members of the Lamiaceae family, namely Salvia officinalis L., Salvia rosmarinus Spenn, Mentha × piperita L., Mentha spicata L. and Origanum vulgare subsp. hirtum (Link) Ietswaart, were studied for the influence of harvesting time on the herb crop yield, the volatile compounds (EOs) content/yield and their chemical composition. EOs were isolated by means of hydro-distillation from different plant parts at different growth stages. Their components were analyzed by gas chromatography coupled with mass spectrometry (GC-MS). The highest yields of EOs were obtained at the full flowering stage and important changes were observed in their composition. The fluctuations in the percentage composition of the major compounds in the EOs, throughout harvesting time, were observed at camphor/α-thujone for S. officinalis, camphor/1,8-cineole for S. rosmarinus, linalool/linalyl acetate and carvone/limonene for M. × piperita and M. spicata, respectively. The chemotype of O. vulgare subsp. hirtum was identified as carvacrol. The optimization of harvesting time could lead to increased crop production and better EOs quality control, with numerous industrial benefits upon the commercial production of such products.
Maturation and Post-Harvest Resting of Fruits Affect the Macronutrients and Protein Content in Sweet Pepper Seeds
Authors: Colombari Lidiane Fernandes , Chamma Larissa , da Silva Gustavo Ferreira , Zanetti Willian Aparecido Leoti , Putti Fernando Ferrari , Cardoso Antonio Ismael Inácio
Published: August 2022

There are few studies about the influence of fruit maturation and post-harvest resting on seed composition, which can be necessary for seedling development and future establishment. Thus, the objective of this study was to evaluate the effect of maturation and post-harvest resting of fruits on the macronutrient and protein content of sweet pepper seeds. The experimental design was a randomized block, with eight treatments, in a 4 × 2 factorial arrangement. The first factor was fruit maturation stages (35, 50, 65 and 80 days after anthesis), and the second, with and without post-harvest resting of the fruits for 7 days. The characteristics evaluated in seeds were the dry weight of one thousand seeds, macronutrient content, and content of albumin, globulin, prolamin and glutelin proteins. There were reductions in K, Ca and Mg content, and an increase in seed content of albumin, globulin and prolamins as a function of the fruit maturation stage. Post-harvest resting of the fruits provided higher Ca content and protein albumin in seeds. The decreasing order of macronutrients and protein content in seeds, independent of fruit maturation and resting stage of the fruits, was N > K > P > Mg > S > Ca, and albumin > globulin ≈ glutelin > prolamine, respectively.
Active Thermoplastic Starch Film with Watermelon Rind Extract for Future Biodegradable Food Packaging
Authors: Todhanakasem Tatsaporn , Jaiprayat Chayanit , Sroysuwan Thunchanok , Suksermsakul Supakanya , Suwapanich Rachit , Maleenont Kamolnate Kitsawad , Koombhongse Piyawit , Young Briana M.
Published: August 2022

Petrochemical plastic wastes generate serious environmental problems because they are resistant to natural decomposition. The aim of this study was to develop a biodegradable active thermoplastic film composed of polyvinyl alcohol (PVA), corn starch (ST), glycerol, and the active compounds from watermelon rind extract (WMRE), or PVA/ST/WMRE, using the casting technique. The film was examined for its mechanical, antioxidant, and functional properties against selected foodborne pathogens. The results showed that the addition of 10% v/v of watermelon rind extract to the film formulation significantly increased the tensile strength from 19.44 ± 0.84 MPa to 33.67 ± 4.38 MPa and slightly increased the percent elongation at break (% EAB) from 35.04 ± 0.96% to 35.16 ± 1.08%. The antioxidant property of PVA/ST/WMRE film was analyzed based on the DPPH scavenging activity assay, which significantly increased from 29.21 ± 0.24% to 63.37 ± 4.27%. The minimum inhibitory concentration (MIC) of watermelon rind extract was analyzed for the growth inhibition of Bacillus cereus ATCC 11778, Escherichia coli ATCC 8739, and Salmonella enterica subsp. enterica serovar Typhimurium ATCC 13311, with 10% (v/v) found as an optimal concentration against B. cereus. Wrapping fresh-cut purple cabbage with PVA/ST/WMRE film significantly reduced the microbial load after 3 days of storage, in comparison to commercial packaging (PET) and thermoplastic control film. Consumer testing of the packaging film indicated that user acceptance of the product was favorable. Therefore, we suggest that this newly developed film can be used as a biodegradable food packaging item that will lead to enhanced food safety, food quality, prolonged shelf life, and consumer acceptance for further food applications.
Performance Evaluation of Ultra-Thin Wearing Course with Different Polymer Modified Asphalt Binders
Authors: Yu Jiangmiao , Chen Yanlin , Wei Xiaopeng , Dong Niya , Yu Huayang
Published: August 2022

Ultra-thin wearing course (UTWC) as an asphalt overlay is widely used in pavement maintenance for extending pavement service life. Researchers focused on improving and evaluating its performance, yet few researchers compare the performance of typical UTWCs. Moreover, some traditional asphalt mixture tests are improper for UTWC due to the thicknesses of UTWC, which is thinner than the traditional asphalt overlay. This study further evaluated the advantages and disadvantages of typical UTWCs. A series of tests were conducted to compare the comprehensive performance of three typical UWTC products, including SMA-10, Novachip-B, and GT-10. Moreover, this study improved the rutting test to evaluate its rutting performance more accurately. Rutting specimens of 20 mm thick and 50 mm thick composite specimens (20 mm UTWC + 30 mm Portland cement concrete slabs) were prepared. Two types of PCC slabs were used, including unprocessed PCC slabs and PCC slabs with preset cracks. The test results showed that Novachip-B showed the best water stability and weakest raveling resistance, while GT-10 showed the best fatigue and anti-skid performance. The rutting performance of UTWCs was reduced because of the influence of preset cracks. The rutting depth of GT-10 was only 60–90% of that of others, showing the comprehensive performance of GT-10 was better than that of others. These results provide a significant reference for the research and application of UTWC.
Determination of Translaminar Notch Fracture Toughness for Laminated Composites Using Brazilian Disk Test
Authors: Torabi Ali Reza , Motamedi Mohammad Amin , Bahrami Bahador , Noushak Meghdad , Cicero Sergio , Álvarez José Alberto
Published: August 2022

This paper evaluates the fracture of notched epoxy matrix composites using the Brazilian disk (BD) test from both numerical and experimental points of view. The study began with a comprehensive experimental program covering three different composite lay-ups (quasi-isotropic, unidirectional, and cross-ply) and various geometries of U and V notches. Specifically, the BD samples combined the three layouts, four different notch angles, and three notch radii with three specimens per combination, leading to an overall number of 108 fracture tests. The experiments showed the appropriateness of the BD test for the study of the fracture behavior of composite materials and provided a good pool of data for further investigations. Subsequently, the virtual isotropic material concept (VIMC) was applied in combination with two fracture criteria to theoretically predict the experimentally acquired fracture loads. This study demonstrated that using the VIMC approach can provide robust predictions while incurring much lower computational costs compared to the conventional approaches found in the literature.
Effect of Polymer Coatings on the Permeability and Chloride Ion Penetration Resistances of Nano-Particles and Fibers-Modified Cementitious Composites
Authors: Zhang Peng , Wang Wenshuai , Lv Yajun , Gao Zhen , Dai Siyuan
Published: August 2022

Nano-particles and fibers-modified cementitious composite (NFCC) can greatly overcome the shortcomings of traditional cementitious materials, such as high brittleness and low toughness, and improve the durability of the composite, which in turn increases the service life of the structures. Additionally, the polymer coatings covering the surface of the composite can exert a good physical shielding effect on the external water, ions, and gases, so as to improve the permeability and chloride ion penetration resistance of the composite. In this study, the effect of three types of polymer coatings on the water contact angle, permeability resistance, and chloride ion penetration resistance of the NFCC with varied water–binder ratios were investigated. Three kinds of polymers (chlorinated rubber coating, polyurethane coating, and silane coating) were applied in two types of coatings, including single-layer and double-layer coatings. Three water–binder ratios of 35 wt.%, 40 wt.%, and 45 wt.% were used for the NFCC. The research results revealed that the surface of the NFCC treated with polymer coatings exhibited excellent hydrophobicity. The permeability height and chloride diffusion coefficient of the NFCC coated with different types of polymer coatings were 31–48% and 36–47% lower, respectively, than those of the NFCC without polymer coatings. The durability of the NFCC was further improved when the polymer coatings were applied to the surface in two-layer. Furthermore, it was discovered that increasing the water–binder ratio of the NFCC would lessen the positive impact of polymer coatings on the durability of NFCC.
Study on Optimization of Initial Support for a Tunnel in the Fracture Zone Based on the Strength Reduction Method
Authors: Ding Ke , Wang Lianguo , Ren Bo , Wang Shuai , Jiang Chongyang
Published: August 2022

In this paper, the fracture zone of the Chunxuan Road Tunnel in Jinan, Shandong Province, China was selected for the engineering background. With reference to the field monitoring data, a finite element model was established based on the strength reduction method. With the aid of this model, the influences of different initial support parameters (including lining, bolt and steel arch) on the tunnel deformation in the fracture zone were analyzed to obtain reasonable initial support parameters. The results show that the tunnel deformation under the original initial support is severe. Among the initial support methods, such as lining, bolt, and steel arch, the parameters of the lining and steel arch significantly influence the tunnel deformation in the fracture zone. Therefore, selecting appropriate initial support parameters can effectively control the tunnel deformation in the fracture zone. After the optimized support scheme is adopted, failure instability no longer occurs in the tunnel and the tunnel deformation is also effectively controlled. The research findings provide a clear reference for deformation control and support scheme optimization of similar projects.
An Optimization of a Turbocharger Blade Based on Fluid–Structure Interaction
Authors: Li Minghai , Li Yuanzhe , Jiang Feng , Hu Jie
Published: August 2022

The structural fracture of the compressor blade is the main cause of fatigue failure. The novelty of this paper is the creative application of bent swept-back modeling to the blade of the turbocharger impeller. This paper is based on a compressor impeller satisfying the k-ε turbulence model. A simulation model was established in ANSYS software, the fluid–structure interaction was calculated in the three models before and after improvement, and the results were compared and analyzed. The optimized blade could improve the blade structure, reduce stress and deformation, and improve the pressurization ratio. In this paper, the optimization scheme of different parameters was discussed in line with the optimal solution. Based on the combination of fuzzy and grey correlation theory, it was concluded that the correlation between pressure and total deformation was higher than that of equivalent stress, and these two values reached 0.8596 and 0.8001, respectively. The results showed that the pressure and total deformation were significantly related to the flow rate. It provides a feasible scheme for further improvement of the supercharger compressor.
Novel Cell Receptor System of Eukaryotes Formed by Previously Unknown Nucleic Acid-Based Receptors
Authors: Tetz Victor , Tetz George
Published: August 2022

Here, our data provide the first evidence for the existence of a previously unknown receptive system formed by novel DNA- and RNA-based receptors in eukaryotes. This system, named the TR-system, is capable of recognizing and generating a response to different environmental factors and has been shown to orchestrate major vital functions of fungi, mammalian cells, and plants. Recently, we discovered the existence of a similar regulatory system in prokaryotes. These DNA- and RNA-based receptors are localized outside of the membrane forming a type of a network around cells that responds to a variety of chemical, biological, and physical factors and enabled the TR-system to regulate major aspects of eukaryotic cell life as follows: growth, including reproduction and development of multicellular structures; sensitivity to temperature, geomagnetic field, UV, light, and hormones; interaction with viruses; gene expression, recognition and utilization of nutrients. The TR-system was also implicated in cell-memory formation and was determined to be responsible for its maintenance and the forgetting of preceding events. This system is the most distant receptive and regulatory system of the cell that regulates interactions with the outer environment and governs the functions of other receptor-mediated signaling pathways.
Absolute Environmental Sustainability of Materials Dissipation: Application for Construction Sector
Authors: Baabou Wafaa , Bjørn Anders , Bulle Cécile
Published: August 2022

The materials used globally in the construction sector are projected to more than double in 2060, causing some to deplete. We argue that access to the services that the resources provide must be protected, thus implying that a carrying capacity (CC) for resource dissipation must be set. Dissipation accrues when the resource becomes inaccessible to users. The CC allows defining a maximum dissipation rate that allows to maintain those resources’ availability in the future. The CC of the dissipation of the resource may be operationalized to characterize the resource use impact, using absolute environmental sustainability assessments principles. The study makes it possible to determine a dissipation CC as the world dissipation rate that would enable all users to adapt to using an alternative resource before the material’s reserve is entirely dissipated. The allocation of a fraction of this CC to the building sector was performed using equal per capita and grandfathering sharing principles. Finally, we applied the method to the case of steel in a school life cycle. The results show that the actual dissipation rates of iron, copper and manganese in the building sector exceed the dissipation CC by 70%, 56% and 68%, respectively. However, aluminum dissipation is 90% less than the assigned CC. The allocation to schools shows that the results are influenced by the choice of allocation principle. The application in the case of steel use of the school life cycle shows an exceedance of the CC that decreases when increasing the building life span.
A New Mortality Framework to Identify Trends and Structural Changes in Mortality Improvement and Its Application in Forecasting
Authors: Fu Wanying , Smith Barry R. , Brewer Patrick , Droms Sean
Published: August 2022

We construct a new age-specific mortality framework and implement an exemplar (DLGC) that provides an excellent fit to data from various countries and across long time periods while also providing accurate mortality forecasts by projecting parameters with ARIMA models. The model parameters have clear and reasonable interpretations that, after fitting, show stable time trends that react to major world mortality events. These trends are similar for countries with similar life-expectancies and capture mortality improvement, mortality structural change, and mortality compression over time. The parameter time plots can also be used to improve forecasting accuracy by suggesting training data periods and appropriate stochastic assumptions for parameters over time. We also give a quantitative analysis on what factors contribute to increased life expectancy and gender mortality differences during different age periods.
Can Machine Learning Algorithms Successfully Predict Grassland Aboveground Biomass?
Authors: Wang Yue , Qin Rongzhu , Cheng Huzi , Liang Tiangang , Zhang Kaiping , Chai Ning , Gao Jinlong , Feng Qisheng , Hou Mengjing , Liu Jie , Liu Chenli , Zhang Wenjuan , Fang Yanjie , Huang Jie , Zhang Feng
Published: August 2022

The timely and accurate estimation of grassland aboveground biomass (AGB) is important. Machine learning (ML) has been widely used in the past few decades to deal with complex relationships. In this study, based on an 11-year period (2005–2015) of AGB data (1620 valid AGB measurements) on the Three-River Headwaters Region (TRHR), combined with remote sensing data, weather data, terrain data, and soil data, we compared the predictive performance of a linear statistical method, machine learning (ML) methods, and evaluated their temporal and spatial scalability. The results show that machine learning can predict grassland biomass well, and the existence of an independent validation set can help us better understand the prediction performance of the model. Our findings show the following: (1) The random forest (RF) based on variables obtained through stepwise regression analysis (SRA) was the best model (R2vad = 0.60, RMSEvad = 1245.85 kg DW (dry matter weight)/ha, AIC = 5583.51, and BIC = 5631.10). It also had the best predictive capability of years with unknown areas (R2indep = 0.50, RMSEindep = 1332.59 kg DW/ha). (2) Variable screening improved the accuracy of all of the models. (3) All models’ predictive accuracy varied between 0.45 and 0.60, and the RMSE values were lower than 1457.26 kg DW/ha, indicating that the results were reliably accurate.