bluuebunny/crossref_metadata_2025 huggingface dataset:
Tissue Proteome of 2-Hydroxyacyl-CoA Lyase Deficient Mice Reveals Peroxisome Proliferation and Activation of ω-Oxidation
Authors: Khalil Youssef , Carrino Sara , Lin Fujun , Ferlin Anna , Lad Heena V. , Mazzacuva Francesca , Falcone Sara , Rivers Natalie , Banks Gareth , Concas Danilo , Aguilar Carlos , Haynes Andrew R. , Blease Andy , Nicol Thomas , Al-Shawi Raya , Heywood Wendy , Potter Paul , Mills Kevin , Gale Daniel P. , Clayton Peter T.
Published: January 2022

Peroxisomal fatty acid α-oxidation is an essential pathway for the degradation of β-carbon methylated fatty acids such as phytanic acid. One enzyme in this pathway is 2-hydroxyacyl CoA lyase (HACL1), which is responsible for the cleavage of 2-hydroxyphytanoyl-CoA into pristanal and formyl-CoA. Hacl1 deficient mice do not present with a severe phenotype, unlike mice deficient in other α-oxidation enzymes such as phytanoyl-CoA hydroxylase deficiency (Refsum disease) in which neuropathy and ataxia are present. Tissues from wild-type and Hacl1−/− mice fed a high phytol diet were obtained for proteomic and lipidomic analysis. There was no phenotype observed in these mice. Liver, brain, and kidney tissues underwent trypsin digestion for untargeted proteomic liquid chromatography-mass spectrometry analysis, while liver tissues also underwent fatty acid hydrolysis, extraction, and derivatisation for fatty acid gas chromatography-mass spectrometry analysis. The liver fatty acid profile demonstrated an accumulation of phytanic and 2-hydroxyphytanic acid in the Hacl1−/− liver and significant decrease in heptadecanoic acid. The liver proteome showed a significant decrease in the abundance of Hacl1 and a significant increase in the abundance of proteins involved in PPAR signalling, peroxisome proliferation, and omega oxidation, particularly Cyp4a10 and Cyp4a14. In addition, the pathway associated with arachidonic acid metabolism was affected; Cyp2c55 was upregulated and Cyp4f14 and Cyp2b9 were downregulated. The kidney proteome revealed fewer significantly upregulated peroxisomal proteins and the brain proteome was not significantly different in Hacl1−/− mice. This study demonstrates the powerful insight brought by proteomic and metabolomic profiling of Hacl1−/− mice in better understanding disease mechanism in fatty acid α-oxidation disorders.
Maternal Fructose Intake Causes Developmental Reprogramming of Hepatic Mitochondrial Catalytic Activity and Lipid Metabolism in Weanling and Young Adult Offspring
Authors: Smith Erin Vanessa LaRae , Dyson Rebecca Maree , Vanderboor Christina M. G. , Sarr Ousseynou , Anderson Jane , Berry Mary J. , Regnault Timothy R. H. , Peng Lifeng , Gray Clint
Published: January 2022

Excess dietary fructose is a major public health concern, yet little is known about its influence on offspring development and later-life disease when consumed in excess during pregnancy. To determine whether increased maternal fructose intake could have long-term consequences on offspring health, we investigated the effects of 10% w/v fructose water intake during preconception and pregnancy in guinea pigs. Female Dunkin Hartley guinea pigs were fed a control diet (CD) or fructose diet (FD; providing 16% of total daily caloric intake) ad libitum 60 days prior to mating and throughout gestation. Dietary interventions ceased at day of delivery. Offspring were culled at day 21 (D21) (weaning) and at 4 months (4 M) (young adult). Fetal exposure to excess maternal fructose intake significantly increased male and female triglycerides at D21 and 4 M and circulating palmitoleic acid and total omega-7 through day 0 (D0) to 4 M. Proteomic and functional analysis of significantly differentially expressed proteins revealed that FD offspring (D21 and 4 M) had significantly increased mitochondrial metabolic activities of β-oxidation, electron transport chain (ETC) and oxidative phosphorylation and reactive oxygen species production compared to the CD offspring. Western blotting analysis of both FD offspring validated the increased protein abundances of mitochondrial ETC complex II and IV, SREBP-1c and FAS, whereas VDAC1 expression was higher at D21 but lower at 4 M. We provide evidence demonstrating offspring programmed hepatic mitochondrial metabolism and de novo lipogenesis following excess maternal fructose exposure. These underlying asymptomatic programmed pathways may lead to a predisposition to metabolic dysfunction later in life.
A Literature Survey of Recent Advances in Chatbots
Authors: Caldarini Guendalina , Jaf Sardar , McGarry Kenneth
Published: January 2022

Chatbots are intelligent conversational computer systems designed to mimic human conversation to enable automated online guidance and support. The increased benefits of chatbots led to their wide adoption by many industries in order to provide virtual assistance to customers. Chatbots utilise methods and algorithms from two Artificial Intelligence domains: Natural Language Processing and Machine Learning. However, there are many challenges and limitations in their application. In this survey we review recent advances on chatbots, where Artificial Intelligence and Natural Language processing are used. We highlight the main challenges and limitations of current work and make recommendations for future research investigation.
Comparison of the Parasitization of Chelonus inanitus L. (Hymenoptera: Braconidae) in Two Spodoptera Pests and Evaluation of the Procedure for Its Production
Authors: Magaña Antonio Jesús , Dáder Beatriz , Sancho Gonzalo , Adán Ángeles , Morales Ignacio , Viñuela Elisa
Published: January 2022

Chelonus inanitus (L.) is an egg-larval parasitoid of noctuids Spodoptera exigua (Hübner) and S. littoralis (Boisduval), whose mass rearing or real potential has not been targeted yet. To improve the rearing in the factitious host Ephestia kuehniella Zeller, we investigated the influence of host age and number of females parasitizing simultaneously on the overall rearing success, the influence of host age on the life cycle, and the influence of host species on the parasitoid body size. The proportion of emerging C. inanitus was higher from young host eggs, but more females emerged from mature eggs. Under high parasitoid competition, we observed a reduction in non-parasitized hosts without reducing parasitoid emergence. The parasitoid life cycle was longer in females, but the mismatch between sexes was smaller in mature eggs. The parasitoid size was smaller in the factitious host than in the natural hosts. Under semi-field conditions, we investigated the competition among parasitoid females on the overall parasitism success. The reproductive parasitism was more successful in S. exigua than in S. littoralis, and the maximum emergence was reached with three and four females, respectively. The control of S. littoralis may be attributed to the high developmental mortality, a non-reproductive parasitism that is often underestimated.
A New Cixiid Fossil Genus of the Tribe Acrotiarini from Mid-Cretaceous Burmese Amber (Insecta, Hemiptera, Fulgoromorpha)
Authors: Wang Menglin , Liang Feiyang , Bourgoin Thierry
Published: January 2022

A new Burmese amber genus Maculixiusgen. nov. with its type species Maculixius jiewenaesp. nov. is described in the planthopper family Cixiidae. This new genus is unique in Burmese Cixiidae by its forewing venation, with Pcu and A1 merging in the middle of clavus, the late bifurcation of ScP+R slightly after this level, and the early CuA forking well before this level. Although the head capsule is missing, it belongs to the recently described tribe Acrotiarini, based on the pentacarinated mesonotum and the distinctly arched RA on forewing with cell C1 wider submedially than apically. Morphological characteristics of Acrotiarini are discussed, and a key to identification of Acrotiarini genera is provided. The new taxon broadens the knowledge of the tribe, and it underlines the already great diversity of the family in the Cretaceous.
Persistency of Indigenous and Exotic Entomopathogenic Fungi Isolates under Ultraviolet B (UV-B) Irradiation to Enhance Field Application Efficacy and Obtain Sustainable Control of the Red Palm Weevil
Authors: Sutanto Koko Dwi , Husain Mureed , Rasool Khawaja Ghulam , Malik Akhmad Faisal , Al-Qahtani Wahidah Hazza , Aldawood Abdulrahman Saad
Published: January 2022

The red palm weevil Rhynchophorus ferrugineus Olivier (Coleoptera: Dryophthoridae) has become a key invasive pest and major threat to the palm tree worldwide. Several entomopathogenic fungi are used in insect biological control programs. In the present study, persistency of different local and exotic fungal isolates of Beauveria bassiana and Metarhizium anisopliae was evaluated under UV-B irradiation with different exposure intervals. Several factors, including ultraviolet (UV) light, significantly decrease germination rate of fungi, as UV penetrates and damages their DNA. Several studies have investigated that UV-resistant conidia germinate better under harsh environmental conditions. Seven local and exotic fungi isolates (“BbSA-1”, “BbSA-2”, “BbSA-3”, “MaSA-1”, “BbIDN-1”, “MaIDN-1”, and “MaIDN-2”) were tested in the current study under UV-B irradiation having different UV exposure times (i.e., 15, 30, 60, 120, 180, 240, and 300 min). The colony-forming unit (CFU) in each isolate was used to calculate the survival rate. Results indicated that survival rate of all the isolates decreased under UV-B irradiation for all exposure times compared to no exposure to UV-B irradiation. The CFU number decreased as the exposure time increased. Fungi isolates “MaSA-1”, “BbSA-1”, “BbSA-2”, “MaIDN-1”, and “MaIDN-2” could persist after 300 min exposure to UV-B, while the remaining isolates, such as “BbIDN-1”, and “BbSA-3”, could not persist after 300 min exposure to UV-B. The ultimate objective of the present research was to explore an ultraviolet-tolerant fungal isolate that might be useful in the field application for the sustainable management of the red palm weevil, which has become a key invasive pest in many regions rather than its native range. Most of the fungus isolates studied in the present work were collected from Saudi Arabia’s Al-Qatif region, where the red palm weevil has infested more than ten thousand trees, worth millions of riyals.
The Utility of Maternal Point of Care Ultrasound on Labor and Delivery Wards
Authors: Algodi Mohammed , Wolfe Diana S. , Taub Cynthia C.
Published: January 2022

Point-of-care ultrasonography (POCUS) refers to limited bedside ultrasound used to evaluate patients for conditions specific to the scope of their practice. Given the benefits of its application, interest in its use is increasing. We aimed to review the literature and assess the potential feasibility of using POCUS of the heart and lungs in the field of obstetrics. We aim to describe its relevance and value as an adjunctive tool for critically ill obstetric patients on labor and delivery wards.
UCHL1 and Proteasome in Blood Serum in Relation to Dietary Habits, Concentration of Selected Antioxidant Minerals and Total Antioxidant Status among Patients with Alzheimer’s Disease
Authors: Bogdan Sylwia , Puścion-Jakubik Anna , Klimiuk Katarzyna , Socha Katarzyna , Kochanowicz Jan , Gorodkiewicz Ewa
Published: January 2022

Alzheimer’s disease (AD) is an incurable neurodegenerative disease. It is the most common form of dementia among the elderly population. So far, no effective methods of its treatment have been found. Research to better understand the mechanism of pathology may provide new methods for early diagnosis. This, in turn, could enable early intervention that could slow or halt disease progression and improve patients’ quality of life. Therefore, minimally invasive markers, including serum-based markers, are being sought to improve the diagnosis of AD. One of the important markers may be the concentration of UCHL1 and the proteasome in the blood serum. Their concentration can be affected by many factors, including eating habits. This study was conducted in 110 patients with early or moderate AD, with a mean age of 78.0 ± 8.1 years. The patients were under the care of the Podlasie Center of Psychogeriatrics and the Department of Neurology (Medical University of Białystok, Poland). The control group consisted of 60 healthy volunteers, matched for gender and age. The concentration of UCHL1 and the 20S proteasome subunit were measured by surface plasmon resonance imaging (SPRI). In addition, a nutritional interview was conducted with patients with AD, which assessed the frequency of consumption of 36 groups of products. In the group of patients with AD, compared to the control group, we showed a significantly higher concentration of UCHL1 (56.05 vs. 7.98 ng/mL) and the proteasome (13.02 vs. 5.72 µg/mL). Moreover, we found a low negative correlation between UCHL1 and the proteasome in the control group, and positive in the AD group. The analysis of eating habits showed that the consumption of selected groups of products may affect the concentration of the tested components, and therefore may have a protective effect on AD.
Nursing Students’ Knowledge, Awareness, and Experiences of Emergency Contraception Pills’ Use
Authors: Leon-Larios Fatima , Ruiz-Ferron Cecilia , Jalon-Neira Rocio-Marina , Praena-Fernández Juan-Manuel
Published: January 2022

The emergency contraception pill (ECP) is a non-prescribed medication in Spain. However, there is not enough evidence of its use among young people to define sex education contents. The aims of this research were to describe the experiences of the use of the ECP in university students and analyze their knowledge, attitude, and awareness regarding the ECP. The cross-sectional, analytic study was conducted with nursing degree students at the University of Seville. A total of 478 students answered the questionnaire. All of the students (100%) had heard about the ECP and had a positive attitude towards this contraceptive. A total of 25.7% had used the ECP, mainly because a condom had failed or because they did not use any contraceptive at all. Deficiencies in knowledge are related with the ECPs’ mechanism of action, efficacy after repeated use, and the type of ECP available. Female students who used no method at all or withdrawal, and who were over 20 years old, used ECP to a greater extent (p < 0.005). Further education initiatives focused on the use of the ECP, its efficacy, and typology are needed, particularly among future health professionals who will later educate other young people.
Micro-Elimination of Hepatitis C among Patients with Kidney Disease by Using Electronic Reminder System—A Hospital-Based Experience
Authors: Su Pei-Yuan , Su Wei-Wen , Hsu Yu-Chun , Wang Shu-Yi , Chiu Ping-Fang , Yen Hsu-Heng
Published: January 2022

Background: Little is known about the use of an electronic reminder system for HCV screening among patients with kidney disease. In this study, we tried to determine whether reminder systems could improve the HCV screening rate in patients with kidney disease. Methods: Patients with kidney disease were enrolled from August 2019 to December 2020 to automatically screen and order HCV antibody and RNA testing in outpatient departments. Results: A total of 19,316 outpatients with kidney disease were included, and the mean age was 66.5 years. The assessment rate of HCV antibody increased from 53.1% prior to the reminder system to 79.8% after the reminder system (p < 0.001), and the assessment rate of HCV RNA increased from 71% to 82.9%. The anti-HCV seropositivity rate decreased from 7.3% at baseline to 2.5% after the implementation of the reminder system (p < 0.001), and the percentage of patients with detectable HCV RNA among those with anti-HCV seropositivity decreased from 69.1% at baseline to 46.8% (p < 0.001). Conclusions: The feasibility of an electronic reminder system for HCV screening among patients with kidney disease in a hospital-based setting was demonstrated.
Role of Cardiac Magnetic Resonance Imaging in the Evaluation of Athletes with Premature Ventricular Beats
Authors: Brunetti Giulia , Cipriani Alberto , Perazzolo Marra Martina , De Lazzari Manuel , Bauce Barbara , Calore Chiara , Rigato Ilaria , Graziano Francesca , Vio Riccardo , Corrado Domenico , Zorzi Alessandro
Published: January 2022

Premature ventricular beats (PVBs) in athletes are not rare. The risk of PVBs depends on the presence of an underlying pathological myocardial substrate predisposing the subject to sudden cardiac death. The standard diagnostic work-up of athletes with PVBs includes an examination of family and personal history, resting electrocardiogram (ECG), 24 h ambulatory ECG (possibly with a 12-lead configuration and including a training session), maximal exercise testing and echocardiography. Despite its fundamental role in the diagnostic assessment of athletes with PVBs, echocardiography has very limited sensitivity in detecting the presence of non-ischemic left ventricular scars, which can be revealed only through more in-depth studies, particularly with the use of contrast-enhanced cardiac magnetic resonance (CMR) imaging. The morphology, complexity and exercise inducibility of PVBs can help estimate the probability of an underlying heart disease. Based on these features, CMR imaging may be indicated even when echocardiography is normal. This review focuses on interpreting PVBs, and on the indication and role of CMR imaging in the diagnostic evaluation of athletes, with a special focus on non-ischemic left ventricular scars that are an emerging substrate of cardiac arrest during sport.
The Clinical Impact of Combining Neutrophil-to-Lymphocyte Ratio with Sarcopenia for Improved Discrimination of Progression-Free Survival in Patients with Colorectal Cancer
Authors: Lee Su Young , Chung Eric , Cho Eun-Suk , Lee Jae-Hoon , Park Eun Jung , Shin Su-Jin , Baik Seung Hyuk , Lee Kang Young , Kang Jeonghyun
Published: January 2022

This study aimed to evaluate the clinical impact of combined sarcopenia and inflammation classification (CSIC) in patients with colorectal cancer (CRC). The skeletal muscle index (SMI) and neutrophil-to-lymphocyte ratio (NLR) were measured in 1270 patients who underwent surgery between January 2005 and April 2014. A Cox proportional hazards model was used to evaluate the correlation of sarcopenia, NLR, and CSIC, with progression-free survival (PFS). The integrated area under the curve (iAUC) was used to compare the discriminatory performance of each model. Using the cut-off values for SMI suggested by Martin et al. and for an NLR of 2.26, the CSIC was defined as follows: nonsarcopenia with low NLR (group 1), nonsarcopenia with high NLR (group 2), sarcopenia with low NLR (group 3), and sarcopenia with high NLR (group 4). Sarcopenia alone was not statistically significant. Multivariate analysis identified that CSIC (group 4 vs. group 1; hazard ratio (HR), 1.726; 95% CI, 1.130–2.634; p = 0.011) and NLR (HR, 1.600; 95% CI, 1.203–2.128; p = 0.001) were independently associated with PFS. The CSIC improved the prediction accuracy of PFS compared with NLR (iAUC mean difference = 0.011; 95% CI, 0.0018–0.028). In conclusion, the combination of sarcopenia and NLR could improve prognostic accuracy, and thus compensate for the limitation of sarcopenia.
The Interrelationship between Sarcoidosis and Atherosclerosis—Complex Yet Rational
Authors: Hoss Sara , Grinberg Tzlil , Eisen Alon
Published: January 2022

Sarcoidosis is a systemic inflammatory disease of unknown etiology, characterized by the presence of non-caseating granulomas in affected organs [...]
Comparison of Ablation Area and Change in Functional Liver Reserve after Radiofrequency Ablation for Hepatocellular Carcinoma Using the arfa® and VIVA® Systems
Authors: Takaya Hiroaki , Namisaki Tadashi , Matsumoto Kazusuke , Suzuki Junya , Murata Koji , Tsuji Yuki , Nakanishi Keisuke , Kaji Kosuke , Kitade Mitsuteru , Noguchi Ryuichi , Yoshiji Hitoshi
Published: January 2022

Radiofrequency ablation (RFA) is recommended in Japan for patients with hepatocellular carcinomas (HCCs) one to three in number and ≤3 cm in size. The arfa® and VIVA® RFA systems are widely used for patients with HCC and this retrospective observational study aims to compare their performances. The study included 365 patients with HCCs one to three in number and ≤3 cm in size who underwent RFA using the arfa® system (arfa® group) or the VIVA® system (VIVA® group). The total bilirubin (T-Bil) level after RFA was higher in the arfa® group than in the VIVA® group. With a 3-cm electrode needle, the longest diameter (Dmax) and the shortest diameter were analyzed and found to be greater in the arfa® group than in the VIVA® group. Furthermore, Dmax with the 2.5-cm electrode needle was greater in the arfa® group than in the VIVA® group. Statistically significant differences in the ablation area and in the T-Bil value after RFA were observed between the groups; however, these differences are not considered clinical problems because the difference in the ablation area was only slight and the Child–Pugh score was the same between the groups. Thus, hepatologists can use either of the RFA systems based on their preference.
Could Candida Overgrowth Be Involved in the Pathophysiology of Autism?
Authors: Herman Anna , Herman Andrzej Przemysław
Published: January 2022

The purpose of this review is to summarize the current acquiredknowledge of Candida overgrowth in the intestine as a possible etiology of autism spectrum disorder (ASD). The influence of Candida sp. on the immune system, brain, and behavior of children with ASD isdescribed. The benefits of interventions such as a carbohydrates-exclusion diet, probiotic supplementation, antifungal agents, fecal microbiota transplantation (FMT), and microbiota transfer therapy (MTT) will be also discussed. Our literature query showed that the results of most studies do not fully support the hypothesis that Candida overgrowth is correlated with gastrointestinal (GI) problems and contributes to autism behavioral symptoms occurrence. On the one hand, it was reported that the modulation of microbiota composition in the gut may decrease Candida overgrowth, help reduce GI problems and autism symptoms. On the other hand, studies on humans suggesting the beneficial effects of a sugar-free diet, probiotic supplementation, FMT and MTT treatment in ASD are limited and inconclusive. Due to the increasing prevalence of ASD, studies on the etiology of this disorder are extremely needed and valuable. However, to elucidate the possible involvement of Candida in the pathophysiology of ASD, more reliable and well-designed research is certainly required.
Safety and Efficacy of Myval Implantation in Patients with Severe Bicuspid Aortic Valve Stenosis—A Multicenter Real-World Experience
Authors: Elkoumy Ahmed , Jose John , Terkelsen Christian J. , Nissen Henrik , Gunasekaran Sengottuvelu , Abdelshafy Mahmoud , Seth Ashok , Elzomor Hesham , Kumar Sreenivas , Bedogni Francesco , Ielasi Alfonso , Dora Santosh K. , Chandra Sharad , Parikh Keyur , Unic Daniel , Wijns William , Baumbach Andreas , Mylotte Darren , Serruys Patrick , Soliman Osama
Published: January 2022

Bicuspid aortic valve (BAV) is the most common valvular congenital anomaly and is apparent in nearly 50% of candidates for AV replacement. While transcatheter aortic valve implantation (TAVI) is a recommended treatment for patients with symptomatic severe aortic stenosis (AS) at all surgical risk levels, experience with TAVI in severe bicuspid AS is limited. TAVI in BAV is still a challenge due to its association with multiple and complex anatomical considerations. A retrospective study has been conducted to investigate TAVI’s procedural and 30-day outcomes using the Myval transcatheter heart valve (THV) (Meril Life Sciences Pvt. Ltd. Vapi, Gujarat, India) in patients with severe bicuspid AS. Data were collected on 68 patients with severe bicuspid AS who underwent TAVI with the Myval THV. Baseline characteristics, procedural, 30-day echocardiographic and clinical outcomes were collected. The mean age and STS PROM score were 72.6 ± 9.4 and 3.54 ± 2.1. Procedures were performed via the transfemoral route in 98.5%. Major vascular complications (1.5%) and life-threatening bleeding (1.5%) occurred infrequently. No patient had coronary obstruction, second valve implantation or conversion to surgery. On 30-day echocardiography, the mean transvalvular gradient and effective orifice area were 9.8 ± 4.5 mmHg and 1.8 ± 0.4 cm2, respectively. None/trace aortic regurgitation occurred in 76.5%, mild AR in 20.5% and moderate AR in 3%. The permanent pacemaker implantation rate was 8.5% and 30-day all-cause death occurred in 3.0% of cases. TAVI with the Myval THV in selected BAV anatomy is associated with favorable short-term hemodynamic and clinical outcomes.
Right Heart Pulmonary Circulation Unit Response to Exercise in Patients with Controlled Systemic Arterial Hypertension: Insights from the RIGHT Heart International NETwork (RIGHT-NET)
Authors: Vriz Olga , Palatini Paolo , Rudski Lawrence , Frumento Paolo , Kasprzak Jarosław , Ferrara Francesco , Cocchia Rosangela , Gargani Luna , Wierzbowska-Drabik Karina , Capone Valentina , Ranieri Brigida , Salzano Andrea , Stanziola Anna , Marra Alberto , Annunziata Roberto , Chianese Salvatore , Rega Salvatore , Saltalamacchia Teresa , Maramaldi Renato , Sepe Chiara , Limongelli Giuseppe , Cademartiri Filippo , D’Andrea Antonello , D’Alto Michele , Izzo Raffaele , Ferrara Nicola , Mauro Ciro , Cittadini Antonio , Ekkehard Grünig , Guazzi Marco , Bossone Eduardo
Published: January 2022

Background. Systemic arterial hypertension (HTN) is the main risk factor for the development of heart failure with preserved ejection fraction (HFpEF). The aim of the study was was to assess the trends in PASP, E/E’ and TAPSE during exercise Doppler echocardiography (EDE) in hypertensive (HTN) patients vs. healthy subjects stratified by age. Methods. EDE was performed in 155 hypertensive patients and in 145 healthy subjects (mean age 62 ± 12.0 vs. 54 ± 14.9 years respectively, p < 0.0001). EDE was undertaken on a semi-recumbent cycle ergometer with load increasing by 25 watts every 2 min. Left ventricular (LV) and right ventricular (RV) dimensions, function and hemodynamics were evaluated. Results. Echo-Doppler parameters of LV and RV function were lower, both at rest and at peak exercise in hypertensives, while pulmonary hemodynamics were higher as compared to healthy subjects. The entire cohort was then divided into tertiles of age: at rest, no significant differences were recorded for each age group between hypertensives and normotensives except for E/E’ that was higher in hypertensives. At peak exercise, hypertensives had higher pulmonary artery systolic pressure (PASP) and E/E’ but lower tricuspid annular plane systolic excursion (TAPSE) as age increased, compared to normotensives. Differences in E/E’ and TAPSE between the 2 groups at peak exercise were explained by the interaction between HTN and age even after adjustment for baseline values (p < 0.001 for E/E’, p = 0.011 for TAPSE). At peak exercise, the oldest group of hypertensive patients had a mean E/E’ of 13.0, suggesting a significant increase in LV diastolic pressure combined with increased PASP. Conclusion. Age and HTN have a synergic negative effect on E/E’ and TAPSE at peak exercise in hypertensive subjects.
Could Bone Biomarkers Predict Bone Turnover after Kidney Transplantation?—A Proof-of-Concept Study
Authors: Magalhães Juliana , Quelhas-Santos Janete , Pereira Luciano , Neto Ricardo , Castro-Ferreira Inês , Martins Sandra , Frazão João Miguel , Carvalho Catarina
Published: January 2022

Aim: Bone disease after kidney transplant (KT) results from multiple factors, including previous bone and mineral metabolism disturbances and effects of transplant-related medications. New biomolecules have been recently associated with the development and progression of the chronic kidney disease–associated bone and mineral disorder (CKD-MBD). These include sclerostin and the soluble receptor activator of nuclear factor-kB ligand (sRANKL). Methods: To better understand the role of biomarkers in post-transplant bone disease, this study was designed to prospectively evaluate and correlate results from the histomorphometric analysis of bone biopsies after KT with emerging serum biomarkers of the CKD-MBD: sclerostin, Dickkopf-related protein 1 (Dkk-1), sRANKL and osteo-protegerin (OPG). Results: Our data shows a significant increase in plasma levels of bioactive sclerostin after KT accompanied by a significant reduction in plasma levels of Dkk-1, suggesting a promotion of the inhibition of bone formation by osteoblasts through the activation of these inhibitors of the Wnt signaling pathway. In addition, we found a significant increase in plasma levels of free sRANKL after KT accompanied by a significant reduction in plasma levels of its decoy receptor OPG, suggesting an enhanced bone resorption by osteoclasts mediated by this mechanism. Conclusions: Taken together, these results suggest that the loss of bone volume observed after KT could be explain mainly by the inhibition of bone formation mediated by sclerostin accompanied by an enhanced bone resorption mediated by sRANKL.
A Clinical Case of COVID-19-Associated Pulmonary Aspergillosis (CAPA), Illustrating the Challenges in Diagnosis (Despite Overwhelming Mycological Evidence)
Authors: White P. Lewis , Springer Jan , Wise Matt P. , Einsele Hermann , Löffler Claudia , Seif Michelle , Prommersberger Sabrina , Backx Matthijs , Löffler Jürgen
Published: January 2022

The COVID-19 pandemic has resulted in large numbers of patients requiring critical care management. With the established association between severe respiratory virus infection and invasive pulmonary aspergillosis (7.6% for COVID-19-associated pulmonary aspergillosis (CAPA)), the pandemic places a significant number of patients at potential risk from secondary invasive fungal disease. We described a case of CAPA with substantial supporting mycological evidence, highlighting the need to employ strategic diagnostic algorithms and weighted definitions to improve the accuracy in diagnosing CAPA.
Endophytic Colletotrichum Species from Aquatic Plants in Southwest China
Authors: Zheng Hua , Yu Zefen , Jiang Xinwei , Fang Linlin , Qiao Min
Published: January 2022

Colletotrichum species are plant pathogens, saprobes, and endophytes in many economically important hosts. Many studies have investigated the diversity and pathogenicity of Colletotrichum species in common ornamentals, fruits, and vegetables. However, Colletotrichum species occurring in aquatic plants are not well known. During the investigation of the diversity of endophytic fungi in aquatic plants in southwest China, 66 Colletotrichum isolates were obtained from aquatic plants there, and 26 of them were selected for sequencing and analyses of actin (ACT), chitin synthase (CHS-1), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), the internal transcribed spacer (ITS) region, and β-tubulin (TUB2) genomic regions. Based on morphological characterization and multi-locus phylogenetic analyses, 13 Colletotrichum species were recognized, namely, C. baiyuense sp. nov., C. casaense sp. nov., C. demersi sp. nov., C. dianense sp. nov., C. fructicola, C. garzense sp. nov., C. jiangxiense, C. karstii, C. philoxeroidis sp. nov., C. spicati sp. nov., C. tengchongense sp. nov., C. vulgaris sp. nov., C. wuxuhaiense sp. nov. Two species complexes, the C. boninense species complex and C. gloeosporioides species complex, were found to be associated with aquatic plants. Pathogenicity tests revealed a broad diversity in pathogenicity and aggressiveness among the eight new Colletotrichum species.
Predicting Venous Thrombosis in Osteoarthritis Using a Machine Learning Algorithm: A Population-Based Cohort Study
Authors: Lu Chao , Song Jiayin , Li Hui , Yu Wenxing , Hao Yangquan , Xu Ke , Xu Peng
Published: January 2022

Osteoarthritis (OA) is the most common joint disease associated with pain and disability. OA patients are at a high risk for venous thrombosis (VTE). Here, we developed an interpretable machine learning (ML)-based model to predict VTE risk in patients with OA. To establish a prediction model, we used six ML algorithms, of which 35 variables were employed. Recursive feature elimination (RFE) was used to screen the most related clinical variables associated with VTE. SHapley additive exPlanations (SHAP) were applied to interpret the ML mode and determine the importance of the selected features. Overall, 3169 patients with OA (average age: 66.52 ± 7.28 years) were recruited from Xi’an Honghui Hospital. Of these, 352 and 2817 patients were diagnosed with and without VTE, respectively. The XGBoost algorithm showed the best performance. According to the RFE algorithms, 15 variables were retained for further modeling with the XGBoost algorithm. The top three predictors were Kellgren–Lawrence grade, age, and hypertension. Our study showed that the XGBoost model with 15 variables has a high potential to predict VTE risk in patients with OA.
Prevention of Post-Transplant Diabetes Mellitus: Towards a Personalized Approach
Authors: Ducloux Didier , Courivaud Cécile
Published: January 2022

Post-transplant diabetes is a frequent complication after transplantation. Moreover, patients suffering from post-transplant diabetes have increased cardiovascular morbidity and reduced survival. Pathogenesis mainly involves beta-cell dysfunction in presence of insulin resistance. Both pre- and post-transplant risk factors are well-described, and some of them may be corrected or prevented. However, the frequency of post-transplant diabetes has not decreased in recent years. We realized a critical appraisal of preventive measures to reduce post-transplant diabetes.
Customer Churn in Retail E-Commerce Business: Spatial and Machine Learning Approach
Authors: Matuszelański Kamil , Kopczewska Katarzyna
Published: January 2022

This study is a comprehensive and modern approach to predict customer churn in the example of an e-commerce retail store operating in Brazil. Our approach consists of three stages in which we combine and use three different datasets: numerical data on orders, textual after-purchase reviews and socio-geo-demographic data from the census. At the pre-processing stage, we find topics from text reviews using Latent Dirichlet Allocation, Dirichlet Multinomial Mixture and Gibbs sampling. In the spatial analysis, we apply DBSCAN to get rural/urban locations and analyse neighbourhoods of customers located with zip codes. At the modelling stage, we apply machine learning extreme gradient boosting and logistic regression. The quality of models is verified with area-under-curve and lift metrics. Explainable artificial intelligence represented with a permutation-based variable importance and a partial dependence profile help to discover the determinants of churn. We show that customers’ propensity to churn depends on: (i) payment value for the first order, number of items bought and shipping cost; (ii) categories of the products bought; (iii) demographic environment of the customer; and (iv) customer location. At the same time, customers’ propensity to churn is not influenced by: (i) population density in the customer’s area and division into rural and urban areas; (ii) quantitative review of the first purchase; and (iii) qualitative review summarised as a topic.
Urban Land and Development Management in a Challenged Developing World: An Overview of New Reflections
Authors: Awuah Kwasi Gyau Baffour , Abdulai Raymond T.
Published: January 2022

The urban development and management challenges of the developing world are well documented in the literature. However, the global built environment landscape is undergoing rapid changes. These changes are steeped in three fundamental imperatives, which have serious implications for the developing world. These imperatives are population growth and rising urbanisation; environmental challenges, particularly climate change and the quest to embrace sustainability as a panacea; and advances in technological development. This paper discusses these three imperatives with the view to teasing out their implications for urban development and management in the developing world. Consistent with the literature, the paper establishes that most of the population growth and rising urbanisation are occurring in the developing world, particularly Africa and Asia, and although these phenomena have the tendency to increase economic density and promote both private and public investment in urban development, especially construction/housing and related infrastructure activities, there are and will be several problems with them. These include land tenure insecurity, lack of access to decent affordable housing and the threat of destruction to heritage sites. Furthermore, environmental challenges such as poor waste management, and climate change are and will remain pressing issues requiring the adoption of sustainability credentials because of legislative requirements, moral suasion, and value addition. Despite the potential disruptive nature of technology with respect to some aspects of the built environment, it is recognised that advances in technology are essential to the achievement of optimal urban development and management outcomes in the developing world. The paper, therefore, recommends better understanding of the socio-economic, cultural, and political forces underlying urban growth in the developing world, factoring in technology and sustainability in urban development and management, and collaboration among relevant actors, particularly government and the private sector, for optimal outcomes.
Improving Hill Farming: From Maize Monocropping to Alternative Cropping Systems in the Thai Highlands
Authors: Senanuch Chiranan , Tsusaka Takuji W. , Datta Avishek , Sasaki Nophea
Published: January 2022

Lately, the Hill Pond Rice System (HPRS) is being promoted as a form of alternative farming systems in selected northern provinces of Thailand, in which the land conversion is designed to maximize rainwater harvesting in farmland consisting of forest trees, water reservoirs, paddy fields, and high-value crop cultivation to serve environmental and livelihood needs. This study employed the double-hurdle model and the tobit technique to investigate the farm-level factors associated with land conversion from maize monocropping to the HPRS using primary data collected from 253 households in Nan, Chiang Mai, Tak, and Lampang Provinces. It was found that education, farming knowledge, understanding benefits of the HPRS, access to water sources, access to advis, and workforce sharing raised the likelihood and extent of farmland conversion into the HPRS. In contrast, perceived complexity of the HPRS, experiences with negative shocks, and land tenure security lowered the likelihood and extent of land conversion. The findings suggest that on-farm collective action should be promoted to mitigate labor constraints in implementation and that access to equipment should be enhanced through HPRS advisors’ visits.
Morphology of Warsaw City Structure Using Urban Indexes and GIS Tools
Authors: Jachimowicz Anna Małgorzata
Published: January 2022

The article presents the process of creating a tool using GIS systems to describe the city’s spatial structure. Therefore, the subject of this research was the method of describing the city structure using non-functional zoning, in conjunction with the use of urban indicators. Using the relationships between the values of urban indicators and individual typologies of buildings, they can be assigned to subsequent zones from the Rural-to-Urban Transect methodology. Therefore, in this article, urban indicators have been defined, thanks to which it is possible to distinguish different typologies of buildings. Next, the relationships between selected indicators and transect zones have been examined, and thanks to the obtained conclusions, transect zones for Warsaw have been defined. Finally, the spatial structure of a selected part of Warsaw has been described using these zones. The purpose of this study is also to initially assess the usefulness of the developed tool for the needs of urban planners.
How Does Land Consolidation Affect Soil Fungal Community Structure? Take Heavy Metal Contaminated Areas in Eastern China for Example
Authors: Lin Yaoben , Yang Haoran , Ye Yanmei , Wen Jiahao , Chen Danling
Published: January 2022

Farmland land consolidation can effectively improve the quality of farmland soil and the agricultural production level, and can effectively guarantee farmland ecology and food security, which has been widely used in the world. A large number of studies have shown that farmland consolidation has certain adjustments to the basic physical and chemical properties of soil and the content of heavy metals. As a key indicator of soil quality and ecological conditions, soil microorganisms play an important role in soil pollution restoration and the promotion of crop growth. However, there are few domestic and foreign studies on how farmland consolidation affects soil microbial properties, and there are no related reports on the mechanism of action between them, which is a blank in the field of agricultural land consolidation and soil microecology, especially in heavy metal contaminated areas. Therefore, we used the DNA sequence technology to compare fungal community structure in farmlands with and without consolidation in heavy metal contaminated areas. Our results showed that (1) farmland consolidation had a significant impact on soil microbial characteristics, which were mainly manifested as changes in microbial biomass, microbial diversity and community structure. (2) Farmland consolidation had an indirect impact on soil fungal community structure by adjusting the soil physical and chemical properties. (3) The impact of heavy metals on the fungal community structure varied significantly under different levels of heavy metal pollution in farmland consolidation areas. When the pollution was at the highest level, there existed 7 fungus genera showing a strong tolerance to heavy metals and consuming a lot of soil nutrients, of which were Melanospora, Pseudeurotium, Guehomyces, Schizothecium, Gibberella, Myrothecium, and Neurospora. In this study, an analytical method was proposed to analyze the effects of farmland consolidation on soil fungi, and the mechanism was discussed from two aspects—soil physical and chemical properties, and heavy metal content. The results shed some light on farmland consolidation, cultivated land quality evaluation and territorial space ecological restoration.
High-Throughput Sequencing Haplotype Analysis Indicates in LRRK2 Gene a Potential Risk Factor for Endemic Parkinsonism in Southeastern Moravia, Czech Republic
Authors: Kolarikova Kristyna , Vodicka Radek , Vrtel Radek , Stellmachova Julia , Prochazka Martin , Mensikova Katerina , Bartonikova Tereza , Furst Tomas , Kanovsky Petr , Geryk Jan
Published: January 2022

Parkinson’s disease and parkinsonism are relatively common neurodegenerative disorders. This study aimed to assess potential genetic risk factors of haplotypes in genes associated with parkinsonism in a population in which endemic parkinsonism and atypical parkinsonism have recently been found. The genes ADH1C, EIF4G1, FBXO7, GBA, GIGYF2, HTRA2, LRRK2, MAPT, PARK2, PARK7, PINK1 PLA2G6, SNCA, UCHL1, and VPS35 were analyzed in 62 patients (P) and 69 age-matched controls from the researched area (C1). Variants were acquired by high-throughput sequencing using Ion Torrent workflow. As another set of controls, the whole genome sequencing data from 100 healthy non-related individuals from the Czech population were used (C2); the results were also compared with the Genome Project data (C3). We observed shared findings of four intron (rs11564187, rs36220738, rs200829235, and rs3789329) and one exon variant (rs33995883) in the LRRK2 gene in six patients. A comparison of the C1–C3 groups revealed significant differences in haplotype frequencies between ratio of 2.09 for C1, 1.65 for C2, and 6.3 for C3, and odds ratios of 13.15 for C1, 2.58 for C2, and 7.6 for C3 were estimated. The co-occurrence of five variants in the LRRK2 gene (very probably in haplotype) could be an important potential risk factor for the development of parkinsonism, even outside the recently described pedigrees in the researched area where endemic parkinsonism is present.
SAXS Reveals the Stabilization Effects of Modified Sugars on Model Proteins
Authors: Piccinini Astra , Lourenço Eva C. , Ascenso Osvaldo S. , Ventura Maria Rita , Amenitsch Heinz , Moretti Paolo , Mariani Paolo , Ortore Maria Grazia , Spinozzi Francesco
Published: January 2022

Many proteins are usually not stable under different stresses, such as temperature and pH variations, mechanical stresses, high concentrations, and high saline contents, and their transport is always difficult, because they need to be maintained in a cold regime, which is costly and very challenging to achieve in remote areas of the world. For this reason, it is extremely important to find stabilizing agents that are able to preserve and protect proteins against denaturation. In the present work, we investigate, by extensively using synchrotron small-angle X-ray scattering experiments, the stabilization effect of five different sugar-derived compounds developed at ExtremoChem on two model proteins: myoglobin and insulin. The data analysis, based on a novel method that combines structural and thermodynamic features, has provided details about the physical-chemical processes that regulate the stability of these proteins in the presence of stabilizing compounds. The results clearly show that some modified sugars exert a greater stabilizing effect than others, being able to maintain the active forms of proteins at temperatures higher than those in which proteins, in the absence of stabilizers, reach denatured states.
RETRACTED: Assessment of 2-Pentadecyl-2-oxazoline Role on Lipopolysaccharide-Induced Inflammation on Early Stage Development of Zebrafish (Danio rerio)
Authors: Di Paola Davide , Natale Sabrina , Gugliandolo Enrico , Cordaro Marika , Crupi Rosalia , Siracusa Rosalba , D’Amico Ramona , Fusco Roberta , Impellizzeri Daniela , Cuzzocrea Salvatore , Spanò Nunziacarla , Marino Fabio , Peritore Alessio
Published: January 2022

Lipopolysaccharide (LPS), or bacterial endotoxin, is an important virulence factor in several human and animal pathologies. Oxazoline of Palmitoylethanolamide (PEAOXA) has shown strong anti-inflammatory activity in several animal models. LPS was applied for 24 h to zebrafish embryos to induce inflammation, and then the anti-inflammatory action of PEAOXA was evaluated for the first time in the zebrafish model (Danio rerio). Different concentrations of PEAOXA were tested for toxicity on zebrafish embryonic development; only the highest concentration of 30 mg/L showed toxic effects. Quantitative RT-PCR was applied to detect Tumor necrosis factor-α, Interleukin 1β, 6, and 8, and members of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB). Exposure to LPS induced an increase in pro-inflammatory cytokines (tumor necrosis factor and interleukin 1, 6, and 8) in both gene and protein expression, as well as an increase of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and the nuclear factor kappa light polypeptide enhancer in B-cells inhibitor (IκBα) gene expression. Furthermore, acute LPS exposure also induced an increase in tryptase release, related to mast cell activity, and in the production of apoptosis-related proteins (caspase 3, bax, and bcl-2). Treatment with PEAOXA 10 mg/L significantly counteracts LPS-induced inflammation in terms of cytokine expression and decreases tryptase release and the apoptosis pathway.