bluuebunny/crossref_metadata_2025 huggingface dataset:
Cardiovascular Indicators of Systemic Circulation and Acute Mountain Sickness: An Observational Cohort Study
Authors: Chen Renzheng , Sun Mengjia , Yang Jie , Liu Chuan , Zhang Jihang , Ke Jingbin , Deng Yuhan , He Chunyan , Yang Yuanqi , Cheng Ran , Yuan Fangzhengyuan , Tan Hu , Gao Xubin , Huang Lan
Published: September 2021

Background: Acute high-altitude (HA) exposure results in blood pressure (BP) and cardiac function variations in most subjects, some of whom suffer from acute mountain sickness (AMS). Several previous studies have found that cardiovascular function indicators are potentially correlated with AMS.

Objectives: This study aims to examine HA-induced cardiovascular adaptations in AMS patients and compare them with healthy subjects. It also aims to investigate the relationship between cardiovascular function indicators and AMS, as well as to provide some insightful information about the prevention and treatment of AMS.

Methods: Seventy-two subjects were enrolled in this cohort study. All the subjects ascended Litang (4,100 m above sea level). They were monitored by a 24-h ambulatory blood pressure (ABP) device and underwent echocardiography examination within 24 h of altitude exposure. The 2018 Lake Louise questionnaire was used to evaluate AMS.

Results: Acute mountain sickness group consisted of more women (17 [60.7%] vs. 10 [22.7%], p = 0.001) and fewer smokers (5 [17.9%] vs. 23 [52.3%], p = 0.003). Compared with subjects without AMS, subjects with AMS had lower pulse pressure (PP) (daytime PP, 45.23 ± 7.88 vs. 52.14 ± 4.75, p < 0.001; nighttime PP, 42.81 ± 5.92 vs. 49.39 ± 7.67, p < 0.001) and lower effective arterial elastance (Ea) (1.53 ± 0.24 vs. 1.73 ± 0.39, p = 0.023). Multivariate regression indicated that female sex (OR = 0.23, p = 0.024), lower daytime PP (OR = 0.86, p = 0.004), and lower Ea (OR = 0.03, p = 0.015) at low altitude (LA) were independent risk factors for AMS. Combined daytime PP and Ea at LA had a high predictive value for AMS (AUC = 0.873; 95% CI: 0.789–0.956). Correlation analysis showed that AMS-induced headache correlated with daytime PP (R = −0.401, p < 0.001) and nighttime PP at LA (R = −0.401, p < 0.001).

Conclusion: Our study demonstrated that AMS patients had a lower PP and Ea at LA. These baseline indicators of vasodilation at LA were closely associated with AMS, which may explain the higher headache severity in subjects with higher PP at LA.
Triple Oxygen Isotope Measurements (Δ'17O) of Body Water Reflect Water Intake, Metabolism, and δ18O of Ingested Water in Passerines
Authors: Sabat Pablo , Newsome Seth D. , Pinochet Stephanie , Nespolo Roberto , Sanchez-Hernandez Juan Carlos , Maldonado Karin , Gerson Alexander R. , Sharp Zachary D. , Whiteman John P.
Published: September 2021

Understanding physiological traits and ecological conditions that influence a species reliance on metabolic water is critical to creating accurate physiological models that can assess their ability to adapt to environmental perturbations (e.g., drought) that impact water availability. However, relatively few studies have examined variation in the sources of water animals use to maintain water balance, and even fewer have focused on the role of metabolic water. A key reason is methodological limitations. Here, we applied a new method that measures the triple oxygen isotopic composition of a single blood sample to estimate the contribution of metabolic water to the body water pool of three passerine species. This approach relies on Δ'17O, defined as the residual from the tight linear correlation that naturally exists between δ17O and δ18O values. Importantly, Δ'17O is relatively insensitive to key fractionation processes, such as Rayleigh distillation in the water cycle that have hindered previous isotope-based assessments of animal water balance. We evaluated the effects of changes in metabolic rate and water intake on Δ'17O values of captive rufous-collared sparrows (Zonotrichia capensis) and two invertivorous passerine species in the genus Cinclodes from the field. As predicted, colder acclimation temperatures induced increases in metabolic rate, decreases in water intake, and increases in the contribution of metabolic water to the body water pool of Z. capensis, causing a consistent change in Δ'17O. Measurement of Δ'17O also provides an estimate of the δ18O composition of ingested pre-formed (drinking/food) water. Estimated δ18O values of drinking/food water for captive Z. capensis were ~ −11‰, which is consistent with that of tap water in Santiago, Chile. In contrast, δ18O values of drinking/food water ingested by wild-caught Cinclodes were similar to that of seawater, which is consistent with their reliance on marine resources. Our results confirm the utility of this method for quantifying the relative contribution of metabolic versus pre-formed drinking/food water to the body water pool in birds.
Efficacy of Intrauterine Perfusion of Cyclosporin A for Intractable Recurrent Spontaneous Abortion Patients With Endometrial Alloimmune Disorders: A Randomized Controlled Trial
Authors: Zhao Long , Qi Lijuan , Fu Jinhua , Bi Shuqin , Li Lin , Fu Yinghui
Published: September 2021

Objective

To explore the therapeutic efficacy of intrauterine perfusion of cyclosporin A (CsA) in intractable recurrent spontaneous abortion (RSA) patients with endometrial alloimmune dysfunction.

Methods

This is a randomized controlled trial (RCT) of patients with intractable RSA with endometrial alloimmune disorders. A total of 201 women were enrolled, all of whom had at least 3 serial abortions and endometrial alloimmune dysfunction. Participants were randomly assigned to two groups. The CsA group (n= 101) received intrauterine infusion of 250 mg CsA on the 3rd and 7th days after menstruation for 2 menstrual cycles, while the placebo group (n= 100) received placebo. The birth of healthy, deformity-free babies was the main study outcome.

Results

In total, 75 (74.26%) women in the CsA group and 59 (59.00%) women in the placebo group gave birth to healthy babies [P= 0.01,OR= 2.01; 95%CI(1.10∼3.65)]. Compared to the placebo group, the CsA group had dramatically lower endometrial CD56+cell and CD57+cell concentrations at the luteal phase of the second menstrual cycle (P< 0.05).

Conclusion

Intrauterine perfusion of CsA was confirmed to be a promising approach for the treatment of intractable alloimmune RSA.
Changes in Root–Shoot Allometric Relations in Alpine Norway Spruce Trees After Strip Cutting
Authors: Nikolova Petia Simeonova , Geyer Jan , Brang Peter , Cherubini Paolo , Zimmermann Stephan , Gärtner Holger
Published: September 2021

Silvicultural interventions such as strip cuttings can change the resource availability of the edge trees. This may alter tree allometry, as light regime, water, and nutrient availability can change at the forest edge. Increased root growth may optimize resource uptake and/or enhance tree anchorage to withstand the altered wind regime. However, little is known about the patterns of the root–shoot allometric responses to strip cuttings. In three alpine stands differing in climate, site productivity, and stand characteristics, we selected 71 Norway spruce trees and took increment cores from stems, root collars, and main roots. This enabled us to study changes in the long-term root-stem allometry for 46 years and short-term allometric responses to intervention. The effects of cutting were compared between edge trees and trees from the stand interior in 10 years before and after the intervention. The long-term allocation to roots increased with stem diameter, with the strongest effects on the regularly managed stand with the tallest and largest trees. These results support the allometric biomass partitioning theory, which postulates resource allocation patterns between different plant organs to depend on plant size. Strip cutting on north-facing slopes boosted edge-tree growth in all plant compartments and enhanced allocation to roots. This change in allometry started 2 years after cutting but disappeared 7–8 years later. In the post-cutting period, the highest root–shoot increase was observed in the small trees independent of the site. This indicates the change in growing conditions to have the strongest effects in formerly suppressed trees. Thus, the effect of such acclimation on the wind firmness of subdominant spruce trees is a question with high importance for optimizing cutting layouts in lowering post-cutting vulnerability to disturbance. The results from this case study contribute to a better understanding of the structural acclimation of spruce trees from high-elevation forests to new forest edges. However, for a more mechanistic understanding of environmental drivers, further analyses of tree-ring stable isotopes are recommended.
Tragic Optimism as a Buffer Against COVID-19 Suffering and the Psychometric Properties of a Brief Version of the Life Attitudes Scale
Authors: Leung Mega M. , Arslan Gökmen , Wong Paul T. P.
Published: September 2021

The Life Attitudes Scale (LAS) was designed to measure tragic optimism (TO)—a distinct type of optimism that could generate hopeless hope even in dire situations according to existential positive psychology (PP 2.0). This study explains why only a faith-based TO could serve as a buffer against suffering at the Nazi death camps as well as the global coronavirus disease 2019 (COVID-19) pandemic. In study 1, the results showed that the factorial structure of a 15-item LAS-Brief (LAS-B), which is a short measure of TO, replicated the original structure of the 32-item long version. The five factors (i.e., affirmation, acceptance, courage, faith, and self-transcendence) provided a good data model fit statistics for LAS-B; the measure had adequate-to-strong internal and latent construct reliability estimates. In study 2, the buffering effect of TO on the association between suffering experiences during COVID-19 and life satisfaction in adults was examined. The results of the studies were consistent with our hypothesis that TO as measured by LAS-B serves as a buffer against the impact of COVID-19 suffering on life satisfaction.
A Randomized Waitlist-Controlled Trial of an Intergenerational Arts and Heritage-Based Intervention in Singapore: Project ARTISAN
Authors: Ho Andy Hau Yan , Ma Stephanie Hilary Xinyi , Tan Michael Koon Boon , Bajpai Ram Chandra
Published: September 2021

Loneliness has become a global major public health concern, with detrimental effects to the young and old. ARTISAN (Aspiration and Resilience Through Intergenerational Storytelling and Art-based Narratives) is a 5-week, 15-h participatory art and group-based intervention that focuses on resilience building and loneliness alleviation among the young and old through a structured multimodal framework held at a museum space. Developed with a Participatory Action Research (PAR) approach, this intervention is evaluated using an open-label waitlist randomized controlled trial design (RCT) comprised of community-dwelling youth and older adults randomized into an intervention group (n = 35) or a waitlist-control group (n = 33). Participants were assessed on standardized self-reported psychometric measures including loneliness, resilience, quality of life, social support, life satisfaction and national identity at three time points. Qualitative data generated during each intervention session as well as acceptability focus groups were recorded and transcribed. Linear mixed modeling analyses revealed that participants in the intervention group experienced improvements in life satisfaction compared to participants in the waitlist-control group (95% CI: 0.22 to 0.77, p < 0.001, Cohen's d = 0.53) immediately after the completion of ARTISAN. Subgroup analyses for youth participants indicated improvements in quality of life (95% CI: 0.16 to 0.52, p < 0.001, d = 1.31) and national identity (95% CI: 0.18 to 0.80, p = 0.002, d = 0.43) in comparison to the waitlist-control group. At 5-weeks follow-up, the intervention group participants continued to experience high levels of life satisfaction (95% CI: 0.04 to 0.42, p = 0.017, d = 0.47), enhancements in resilience (95% CI: 0.07 to 0.55, p = 0.011, d = 0.46), as well as a significant reduction in loneliness (95% CI: −0.34 to −0.08, p = 0.001, d = 0.61) compared to baseline, reflecting the effectiveness and positive residual effects of the ARTISAN intervention. Similarly, the qualitative findings provided support for the intervention and additional insights to the quantitative findings. This holistic intervention framework that integrates stories, arts and heritage for bridging and empowering lives fills a critical gap in knowledge and practice between the arts, health and citizenship, paving the way for further research in creating a more caring and inclusive society with the arts.

Clinical Trials Registration:www.ClinicalTrials.gov, identifier: NCT03048708.
How to Repurpose the University: A Resilience Lens on Sustainability Governance
Authors: Robinson Zoe P. , Laycock Pedersen Rebecca
Published: September 2021

Universities have an important role in moving society towards a more sustainable future. However, this will require us to repurpose universities, reorienting and refocusing the different university domains (education, research, campus, and outreach) towards sustainability. The governance structures and processes used to embed sustainability into the activities and operations of the institution are critical to achieving the required transformation. Our current university systems which are seen as contributing to socio-ecological system unsustainability are resilient to change due to slow variables such as organisational and sector-wide prevailing paradigms and culture. Therefore, to repurpose a university requires us to destabilise our prevailing system, crossing a threshold into a new stable system of a ‘sustainable university' across all its domains. This paper utilises an adaptation of Biggs et al. (2012) resilience principles for the governance of social-ecological systems to provide a framework to consider aspects of university governance for sustainability that can be utilised to repurpose universities towards sustainability, and destabilize unsustainable elements of the system. This paper draws out examples relating to sustainability governance within universities with regards to the four principles of (i) managing diversity and redundancy, (ii) managing connectivity, (iii) managing slow variables and feedbacks, and (iv) encouraging learning and experimentation within the context of complex adaptive systems. In this article, we have shown that using resilience in a non-normative way is possible (to decrease resilience of an unsustainable system), and that it can also be valuable to help understand how to shift organisational governance towards a particular end-state (in this case, university governance that advances sustainability). This paper provides an example of how to operationalise resilience principles of relevance to the resilience literature as well as providing a practical framework to guide higher education institution governance for sustainability.
Segment Drift Control with a Supervision Mechanism for Autonomous Vehicles
Authors: Liu Ming , Leng Bo , Xiong Lu , Yu Yize , Yang Xing
Published: September 2021

Stable maneuverability is extremely important for the overall safety and robustness of autonomous vehicles under extreme conditions, and automated drift is able to ensure the widest possible range of maneuverability. However, due to the strong nonlinearity and fast vehicle dynamics occurring during the drift process, drift control is challenging. In view of the drift parking scenario, this paper proposes a segmented drift parking method to improve the handling ability of vehicles under extreme conditions. The whole process is divided into two parts: the location approach part and the drift part. The model predictive control (MPC) method was used in the approach to achieve consistency between the actual state and the expected state. For drift, the open-loop control law was designed on the basis of drift trajectories obtained by professional drivers. The drift monitoring strategy aims to monitor the whole drift process and improve the success rate of the drift. A simulation and an actual vehicle test platform were built, and the test results show that the proposed algorithm can be used to achieve accurate vehicle drift to the parking position.
Pattern-Moving-Based Partial Form Dynamic Linearization Model Free Adaptive Control for a Class of Nonlinear Systems
Authors: Li Xiangquan , Xu Zhengguang
Published: September 2021

This work addresses a pattern-moving-based partial form dynamic linearization model free adaptive control (P-PFDL-MFAC) scheme and illustrates the bounded convergence of its tracking error for a class of unknown nonaffine nonlinear discrete-time systems. The concept of pattern moving is to take the pattern class of the system output condition as a dynamic operation variable, and the control purpose is to ensure that the system outputs belong to a certain pattern class or some desired pattern classes. The P-PFDL-MFAC scheme mainly includes a modified tracking control law, a deviation estimation algorithm and a pseudo-gradient (PG) vector estimation algorithm. The classification-metric deviation is considered as an external disturbance, which is caused by the process of establishing the pattern-moving-based system dynamics description, and an improved cost function is proposed from the perspective of a two-player zero-sum game (TP-ZSG). The bounded convergence of the tracking error is rigorously proven by the contraction mapping principle, and the validity of the theoretical results is verified by simulation examples.
Antecedents and Consequences of Digital Entrepreneurial Ecosystems in the Interaction Process with Smart City Development
Authors: Gorelova Irina , Dmitrieva Diana , Dedova Mariya , Savastano Marco
Published: September 2021

The nature of entrepreneurship and its developmental paths in the urban environment are extensively studied in the scientific literature. With a rising interest of scholars in the smart city phenomenon, the role entrepreneurship plays in the development of smart cities became a central topic in academia. However, there is a lack of discussion concerning the specific settings and characteristics of digital entrepreneurship in the smart city scenario. Nowadays, the concept of digital entrepreneurship is considered as a part of the digital entrepreneurial ecosystems (DEE) that provides an environment for effective entrepreneurial activities. Hence, the investigation on how DEE is interconnected with smart cities and how they both can contribute to their mutual development appears both timely and necessary. To reach this research objective, the authors, after giving a clear definition of each component of DEE based on an extensive literature review, consider its interconnection with the smart city model. The connection between the dimensions of a smart city and the structural constituents of DEE is also tracked, highlighting the contribution of each element to the development of a smart city. Through the creation of a comprehensive framework, the results of the paper show clearly that DEE is an inevitable part of a smart city environment. The research also covers the model of DEE engagement in smart city architecture.
Agonistic Interactions between Littermates Reappear after Mixing Multiple Litters at Weaning in Pigs
Authors: Prevolnik Povše Maja , Mesarec Nikolina , Skok Janko , Škorjanc Dejan
Published: September 2021

Weaning involves mixing of unfamiliar litters and is accompanied by an outbreak of aggression, which is usually attributed to between-litter (BL) interactions. In the present study, we thus focused on post-weaning agonism (fighting and mounting) between littermates (within-litter, WL). Two litters were weaned into two pens separated by an empty pen and connected by narrow passages that were opened 24 h after weaning. WL interactions accounted for 38% and 68% of all fights and mounts, respectively, during a 7-day experimental period. After the passages were opened, not only BL, but also WL interactions increased significantly (by 6- to 7-fold on the first day of mixing). WL fights then gradually decreased, while WL mounts continued to increase. During the experimental period, the proportion of both WL fights and mounts decreased. The majority of WL fights (≈80%) and mounts (≈65%) occurred in home pens. A significantly higher percentage of draws was found in WL fights (50% of initiator wins and 41% of draws) compared to BL fights (80% of initiator wins and 11% of draws). Results also showed less asymmetry in the body weight of piglets involved in WL interactions. Mixing of litters at weaning was shown to involve not only intense interactions between unfamiliar piglets but also the recurrence of agonistic interactions between littermates, which is something we should keep in mind when pursuing the highest standards of weaner welfare.
Drought Stress Study on Nicotiana tabacum L., “Baladi”, an In Vitro Experimental Model
Authors: Antofie Maria-Mihaela , Sava Sand Camelia
Published: September 2021

Crops drought tolerance is a trait of outmost importance for agriculture especially today when climate change is affecting more the production for food and feed. The scope of this article is to evaluate in vitro drought stress response of Nicotiana tabacum L., “Baladi”. The experiment was set up for four successive stages starting with in vitro seedling development, hypocotyl cultivation, three generations of micropropagation, pre-acclimatization and acclimatization. The effect of abscisic acid (ABA) and/or polyethylene-glycol 6000 (PEG) on tobacco hypocotyl caulogenesis and micropropagation were investigated. Superoxide-dismutases (SODs) and peroxidases (POXs) are more active and different isoforms patterns have been identified compared to the control for cualogenesis. A decrease of internodes length and a higher shoots multiplication rate were observed. However, under PEG treatment plantlets expressed hyperhydration and ceased rooting. Pre-treatments effects study of ABA and/or PEG were finalized in acclimatization phase for 18 tobacco clones. A summary of our results revealed that ABA and/or PEG induce among others a higher oxidative stress compared to the control in the first stage that is not maintained for all clones until acclimatization. Certain clones expressed a lower SOD activity compared to the control during acclimatization but maintaining higher POX activity.
Design, Development and Testing of Feeding Grippers for Vegetable Plug Transplanters
Authors: Jorg Oliver , Sportelli Mino , Fontanelli Marco , Frasconi Christian , Raffaelli Michele , Fantoni Gualtiero
Published: September 2021

Vegetable transplanting is an important and advantageous practice in vegetables production systems. In recent years, the development of vegetable transplanting tools has increased, as well as the interest for automatic and robotic transplanters. However, at present, the feeding of transplanting machines is often still performed by hand. This paper presents the design, development and testing of a needle gripper and a two-finger gripper for vegetable transplanting. Both grippers were self-designed and tested for picking, lifting and transplanting plug seedlings. Tests have been conducted on fennel (Foeniculum vulgare L.), leek (Allium ampeloprasum L.) chicory (Cichorium intybus L.) and lettuce (Lactuca sativa L.) seedlings to determine the impact that gripper typology might have on the further growth of plants after transplanting. The average success rate of the two-finger gripper in the transplanting experiment was 95% and of the needle gripper 81.75%, respectively. Although neither gripper typology affected the growth of the seedlings after transplanting, several design implications were identified in order to improve the performance of both grippers. Furthermore, the two-finger gripper is more reliable for lettuce and chicory, while the needle gripper requires root plugs with higher firmness and cohesion to prevent shattering.
Comparison of Ambrosia L. Pollen Seasons in Lublin (Poland) and Ivano-Frankivsk (Ukraine) and Presentation of the Morphotypes of Trichomes on A. artemisiifolia L. Shoots in Terms of the Allergenic Properties of the Plant
Authors: Piotrowska-Weryszko Krystyna , Weryszko-Chmielewska Elżbieta , Melnychenko Galyna , Konarska Agata , Mylenka Myroslava , Prokopiv Nataliia , Bożek Małgorzata
Published: September 2021

Ambrosia pollen contains strong allergens. Allergic reactions can also be caused by direct contact with the plant. The investigations of the dynamics of Ambrosia pollen seasons were conducted in Lublin (Poland) and Ivano-Frankivsk (Ukraine) in 2013–2015. The onset and end of the seasons, maximum concentrations, annual sums, and the number of days with an allergy risk were determined. Additionally, the types of trichomes present on different parts of Ambrosia artemisiifolia L. shoots were determined using light microscopy. Morphometric studies were carried out on trichomes sampled from staminate inflorescences. The maximum concentrations and annual sums of Ambrosia pollen were shown to be substantially higher in Ivano-Frankivsk than in Lublin. Similarly, the risk of allergies is higher in the study site in Ukraine. The study results indicate that the presence of Ambrosia pollen grains in Lublin may be associated with long-distance transport. The presence of non-glandular and glandular trichomes was found on the examined organs. The staminate inflorescences were covered by two types of non-glandular trichomes (short and long) and two types of glandular trichomes (linear and biseriate), whose secretory product can cause dermatitis in sensitive subjects upon contact with the plant.
Seasonal Variation of Antioxidant Capacity, Phenols, Minerals and Essential Oil Components of Sage, Spearmint and Sideritis Plants Grown at Different Altitudes
Authors: Chrysargyris Antonios , Evangelides Efstathios , Tzortzakis Nikolaos
Published: September 2021

Medicinal and aromatic plants are well appreciated for their antioxidant and biocidal activities, while great variation on these activities can be related to the species, environmental conditions and harvesting period. In the present study, the seasonal variation of the antioxidant activity, mineral content, yield and chemical composition of the essential oils (EOs) of sage (Salvia officinalis L.), sideritis (Sideritis perfoliata L. subsp. perfoliata) and spearmint (Mentha spicata L.) were tested under two different environmental conditions, each with a different altitude (namely mountainous and plain). Season affected total phenolic content and antioxidant capacity with increased values during winter and lower values during summer period. In summer, plants accumulated more Fe and had higher EO yield, while P and Na were accumulated more in winter. Altitude had a lesser effect on antioxidant capacity of the plants; however, increased minerals (N, K, Na and Ca) accumulation was found in plain areas. Sage plants had the highest antioxidant capacity, Zn content and EO yield. Sideritis had increased Fe content and spearmint plants revealed high N, Na and Mg levels. Furthermore, altitude and season had an impact on the content of main EOs components in all species. FRAP and ABTS were variably correlated with total phenols and minerals, depending on the species, season and altitude. In few cases, antioxidant activity was found to be inversely linked to some EO components (e.g., α-thujone in sage). Finally, the antioxidant content, minerals and EO yield and composition of the examined MAPs were all altered by season and altitude. These findings can be utilized to implement sage, sideritis and spearmint farming in specific ecosystems, determining the season and areas for harvesting the plants, in order to produce high-value products.
Temperature-Based Grapevine Ripeness Modeling for cv. Touriga Nacional and Encruzado in the Dão Wine Region, Portugal
Authors: Rodrigues Pedro , Pedroso Vanda , Gonçalves Fernando , Reis Samuel , Santos João A.
Published: September 2021

In the present study, we calibrated and validated thermal models to predict the DOY date at which the grape maturity index, potential alcohol/total acidity (PA/TA), reaches 0.75 (MS0.75), 1.0 (MS1), 1.5 (MS1.5), and 2.0 (MS2) for two grapevine Portuguese varieties, Touriga Nacional (TN) and Encruzado (EN), growing in the Dão wine region, Portugal. Daily rates of forcing calculated with the Sigmoid (SM) function and the Degree Day (DD) function were used. The outcomes show that the best performance of the models was obtained for the heat accumulation starting at flowering (tx = EL23). The analysis of model sensitivity to changes in forcing rate coefficients (T0, e, and d) enabled the selection of the same models for all maturity stage of each variety. The selected models revealed significant predictability, though dependent on the grape maturity stage and variety (EFF > 0.81 for TN and EFF > 0.75 for EN). The non-linear regression analyses of sugar concentration (SC) and total acidity (TA) with heat accumulation, calculated using the select models, demonstrated that a high fraction of SC and TA variance was explained by the variation of these temperature-based indices. Comparatively to SC and TA, the results highlight that the thermal conditions accumulated from flowering had a lower influence on pH juice variance.
Use of Wild Relatives in Durum Wheat (Triticum turgidum L. var. durum Desf.) Breeding Program: Adaptation and Stability in Context of Contrasting Environments in Tunisia
Authors: Ayed Sourour , Bouhaouel Imen , Othmani Afef , Bassi Filippo Maria
Published: September 2021

In Mediterranean regions, the performance of durum wheat (Triticum turgidum L. var. durum Desf.) yield often varies due to significant genotype × environment interaction (GEI); therefore, yield stability is an important consideration in breeding programs. The aim of this research was to explore the GEI pattern and yield stability of 24 promising durum wheat lines, selected by ICARDA in several African countries (seven elites, four commercial varieties, and 13 durum wheat wide crosses, generated by hybridization of elites and Triticum dicoccoides Koern. ex Schweinf., Triticum araraticum Jakubz, and Aegilops speltoides Tausch) against a Tunisian local check variety ‘Salim’. Yield assessment was conducted across six environments under rainfed conditions, at the field station of Kef in a semi-arid region during four cropping seasons (2014–2015, 2015–2016, 2016–2017, and 2017–2018) and in a sub-humid region at the station of Beja during two cropping seasons (2015–2016 and 2018–2019). The analysis of variance showed that the environment is the main source of variation of grain yield (72.05%), followed by the interaction environments × genotypes (25.33%) and genotypes (2.62%). The genotype × genotype by environment model (PC) based on grain yield identified a mega-environment including Kef (2016–2017 and 2017–2018) and Beja (2015–2016 and 2018–2019) and elite line 22 as a widely adapted genotype. Combined analysis, computed using the average grain yield of lines and the yield stability wide adaptation index (AWAI), showed that elite lines 9 and 23 (2.41 and 2.34 t·ha−1, respectively), and wild relative-derived lines, 5, 1, and 10 (2.37, 2.31, and 2.28 t·ha−1, respectively) were more stable and better yielding than the national reference (2.21 t·ha−1). This finding supports the good yield potential of wild relative-derived lines. The five selections are recommended to be developed in multi-environments in several regions of Tunisia, especially in semi-arid area.
Co-Occurrence of Domestic Dogs and Gastropod Molluscs in Public Dog-Walking Spaces and Implications for Infection with Angiostrongylus vasorum: A Preliminary Study
Authors: Tolhurst Bryony A. , Overall Andrew D. J. , King Peter J. , Morgan Eric R. , Baker Rowenna J.
Published: September 2021

Angiostrongylusvasorum is a helminth parasite of domestic dogs that is increasing in range and prevalence. Its lifecycle requires terrestrial gastropod mollusc (“gastropod”) intermediate hosts, but research is lacking regarding contact risk in situ. We studied co-occurrence between dogs and gastropods in dog-walking spaces in an A. vasorum hotspot in southern England, United Kingdom, with the aim of quantifying environmental and spatio-temporal overlap. We surveyed 390 quadrats and 180 point-counts along 3 km transects at seven sites, yielding 1672 gastropod and 763 dog observations. Common gastropods comprised Arion, Cornu, Monacha, Deroceras, Tandonia, Cochlicella, and Trochulus species. Habitat was the most important factor structuring both gastropod and dog presence and abundance. Likelihood ratio comparisons from conditional probability trees revealed that dogs were 15× more likely to be present on hardstanding surfaces than other habitats but were also present on natural and amenity grassland. Presence of gastropod species associated with high A. vasorum prevalence was 65.12× more likely in woodland/scrub and 62.17× more likely in amenity grassland than other habitats. For gastropods overall, high abundance was 5.82× more likely in woodland/scrub and natural grassland. The findings suggest co-occurrence is highest in amenity and natural grassland, but infection risk is greatest in amenity grassland and woodland/scrub.
Is the Prevalence of Leishmania infantum Linked to Breeds in Dogs? Characterization of Seropositive Dogs in Ibiza
Authors: Edo Maria , Marín-García Pablo Jesús , Llobat Lola
Published: September 2021

Leishmaniosis is an important zoonotic protozoan disease primarily spread to the Mediterranean region by Leishmania infantum, the predominant protozoan species, which accounts for the majority of cases. Development of disease depends on the immune response of the definitive host and, predictably, their genetic background. Recent studies have revealed breed-typical haplotypes that are susceptible to the spread of the protozoan parasite. The objective of this study was to analyze the prevalence of leishmaniosis on a Mediterranean island and determine the relationship between disease prevalence and breed. In addition, information on seropositive animals was recorded to characterize animals affected by the disease. To study the prevalence, a total of 3141 dogs were analyzed. Of these, the 149 infected animals were examined for age, sex, antibody titer, and disease stage. We observed a prevalence of 4.74%, which varied between breeds (p < 0.05). The Doberman Pinscher and Boxer breeds had the highest prevalence of leishmaniosis. Significant differences were observed between breeds with common ancestors, emphasizing the important genetic component. Finally, regarding the characterization of seropositive animals, the distribution is similar to other studies. We discovered a relationship (p < 0.05) between the number of antibody titers and the clinical disease stage, which was also present in Leishmania infantum, suggesting that the development of the disease depends on the humoral or Th2 immune response with ineffective antibodies.
Evaluation of Diet Supplementation with Wheat Grass Juice on Growth Performance, Body Composition and Blood Biochemical Profile of Carp (Cyprinus carpio L.)
Authors: Barbacariu Cristian-Alin , Burducea Marian , Dîrvariu Lenuta , Oprea Eugen , Lupu Andrei-Cristian , Teliban Gabriel-Ciprian , Agapie Alina Laura , Stoleru Vasile , Lobiuc Andrei
Published: September 2021

Wheat grass juice (WGJ) is an extract of young wheat plantlets (Triticum aetivum L.) used worldwide for its health related properties. In this study, the following feeds containing WGJ were tested on common carp (Cyprinus carpio L.): Control (C), WGJ1% (V1), WGJ2% (V2) and WGJ4% (V3) w/w. Fish with an average initial weight of 102 g/individual were grown in a recirculating aquaculture system. The results showed that WGJ had stimulatory effects on growth performance. Accordingly, final body weight increased by 11% at V1, 39% at V2 and 23% at V3, while other indices (feed conversion ratio, specific growth rate, relative growth rate, protein efficiency ratio, and condition factor) were unaffected. Body composition analyses revealed a significant decrease in fat content at V2 and a significant increase in collagen and ash at the same variant, while the protein content was unmodified. Regarding the blood profile, significant increases in the content of albumin, globulin, total protein, and calcium were recorded in the variants with WGJ. The positive results of WGJ on carp can be attributed to its biochemical composition, which is rich in chlorophyll (4.71 mg mL−1), total phenols (164 µg mL−1 gallic acid equivalents), and high antioxidant activity (67% inhibition of DPPH 2,2-diphenyl-1-picrylhydrazyl). The results suggest WGJ can be used as a promising feed additive for common carp.
Supplementing the Diet of Dairy Goats with Dried Orange Pulp throughout Lactation: I. Effect on Milk Performance, Nutrient Utilisation, Blood Parameters and Production Economics
Authors: Guzmán José Luis , Martín-García Ignacio , Pérez-Écija Alejandro , García-Brenes Manuel David , Zarazaga Luis Ángel , Delgado-Pertíñez Manuel
Published: September 2021

Dried orange pulp (DOP) can be incorporated into ruminant diets, but no reports have considered this strategy during the entire lactation period in goats. Two experiments were performed using lactating Payoya goats. In experiment 1, to study the effect, over 180 days, of DOP on milk yield and composition, blood metabolites and economic values, 44 primiparous goats were allocated into three groups: control diet (concentrate plus lucerne) and DOP40 and DOP80 diets, in which DOP replaced 40% and 80%, respectively, of the cereals. Nutrient digestibility and rumen fermentation were also studied (experiment 2). The DOP diets did not affect milk yield and composition. DOP triggered lower intake and digestibility of ether extract and crude protein. Ruminal fermentation was unaffected by DOP, except for a decrease in butyrate for DOP80. The energy balance was unaltered by diet while the balance and retention of nitrogen decreased. Regarding plasma biochemistry, DOP supplementation caused changes that could indicate an improvement in hepatic function and reduced muscular damage and oxidative muscular stress. Moreover, DOP80 provided a profit increase of EUR 3.27/goat. In conclusion, the partial replacement of cereals by DOP is a profitable and healthy nutritional strategy in dairy goats and is suitable for the entire lactation period without compromising productivity.
Jejunal Transcriptomic Profiling for Differences in Feed Conversion Ratio in Slow-Growing Chickens
Authors: Sinpru Panpradub , Riou Cindy , Kubota Satoshi , Poompramun Chotima , Molee Wittawat , Molee Amonrat
Published: September 2021

Improving feed efficiency is an important breeding target for the poultry industry; to achieve this, it is necessary to understand the molecular basis of feed efficiency. We compared the jejunal transcriptomes of low- and high-feed conversion ratio (FCR) slow-growing Korat chickens (KRs). Using an original sample of 75 isolated 10-week-old KR males, we took jejunal samples from six individuals in two groups: those with extremely low FCR (n = 3; FCR = 1.93 ± 0.05) and those with extremely high FCR (n = 3; FCR = 3.29 ± 0.06). Jejunal transcriptome profiling via RNA sequencing revealed 56 genes that were differentially expressed (p < 0.01, FC > 2): 31 were upregulated, and 25 were downregulated, in the low-FCR group relative to the high-FCR group. Functional annotation revealed that these differentially expressed genes were enriched in biological processes related to immune response, glutathione metabolism, vitamin transport and metabolism, lipid metabolism, and neuronal and cardiac maturation, development, and growth, suggesting that these are important mechanisms governing jejunal feed conversion. These findings provide an important molecular basis for future breeding strategies to improve slow-growing chicken feed efficiency.
Traumatic Injuries Detected during Post-Mortem Slaughterhouse Inspection as Welfare Indicators in Poultry and Rabbits
Authors: Valkova Lenka , Voslarova Eva , Vecerek Vladimir , Dolezelova Petra , Zavrelova Veronika , Weeks Claire
Published: September 2021

The findings of traumatic injuries during post-mortem inspection in slaughterhouses reflect the level of pre-slaughter handling of animals at the farm and during transport to the slaughterhouse. The prevalence of traumatic injuries was monitored in poultry (1,089,406,687 broiler chickens, 20,030,744 laying hens, 1,181,598 turkeys, 37,690 geese, 28,579,765 ducks) and rabbits (1,876,929) originating from farms in the Czech Republic and slaughtered in slaughterhouses in the Czech Republic between 2010 and 2019. The greatest incidence of traumatic injuries was found in laying hens (2.80%) and rabbits (1.52%); while the overall incidence of trauma was less than 0.5% in other species and categories. The results show that the current rearing conditions and/or pre-slaughter handling of poultry and rabbits particularly affect the limbs; traumatic findings were significantly (p < 0.01) more frequent on the limbs than on the trunk in all species studied. In poultry, traumatic findings on the trunk were orders of magnitude lower to negligible, so the focus should be on preventing injuries to the limbs. In rabbits, the difference was less pronounced, and many injuries were found on both limbs (0.83%) and trunk (0.69%). Our results emphasize the need to reconsider both housing and pre-slaughter handling methods to determine minimum standards for the protection of rabbits, which are still lacking in European legislation.
Abnormal Prothrombin (PIVKA-II) Expression in Canine Tissues as an Indicator of Anticoagulant Poisoning
Authors: Maniscalco Lorella , Varello Katia , Zoppi Simona , Abbamonte Giuseppina , Ferrero Marta , Torres Elena , Ostorero Federica , Rossi Francesca , Bozzetta Elena
Published: September 2021

PIVKA-II is an aberrant form of vitamin K that has been demonstrated to be increased in human coagulation disorders and in some neoplastic diseases. In veterinary medicine, PIVKA-II levels have been demonstrated to be useful for distinguishing anticoagulant poisoning from other coagulopathies. In forensic pathology, there is the need to distinguish malicious poisoning from other causes of death and, in some cases, identifying poisoned dogs from dogs that died as a result of other coagulative disorders can be challenging. In this study, dogs that suddenly died underwent necropsy, histological examination, and toxicological analysis to establish cause of death. PIVKA-II immunohistochemical expression was evaluated on hepatic and renal tissues, and on neoplastic lesions when present. A total of 61 dogs were analyzed and anticoagulant substances were identified in 16 of the 61. Immunolabelling for PIVKA-II was observed in 27 of 61 cases in the liver and in 24 of 61 cases in the kidneys. Among the poisoned dogs, the PIVKA-II expression was present in the liver in 15 of 16 cases and in the kidneys in 16 of 16. Neoplastic lesions represented mainly by haemangiosarcomas were negative. This study highlights how the immunohistochemical expression of PIVKA-II in hepatic and renal tissues can be useful to identify patients with coagulative disorders due to clinical condition or the ingestion of anticoagulants substances.
Synthetic Olfactory Agonist Use in the Farrowing House to Reduce Sow Distress and Improve Piglet Survival
Authors: Terry Robyn , Nowland Tanya L. , van Wettere William H. E. J. , Plush Kate J.
Published: September 2021

The aim of the study was to investigate if the application of a synthetic olfactory agonist (SOA) would reduce indicators of stress in sows, in response to a stressor prior to parturition, and if it would improve farrowing house performance of sows and their piglets. Two studies were conducted: an intensive study with 47 sows, either having their first or second litter (Control n = 24; SOA n = 23); and a commercial validation study with 418 sows, either having their first litter or have had multiple litters (Control n = 210; SOA n = 208). Within the intensive study, sows were housed with or without a synthetic olfactory agonist suspended in the creep area of the farrowing crate, whereas within the commercial validation study, sows were housed with or without a synthetic olfactory agonist suspended over the adjoining creep area of two farrowing crates. Within the intensive study, despite a discernible increase in cortisol concentration in response to a stressor (snout rope test), cortisol response was not different between treatments (p > 0.05). Farrowing duration in first-litter sows exposed to the SOA was decreased (p < 0.001) whilst there was no impact on farrowing duration in second litter sows. Piglets were not attracted by the SOA to increase their utilisation of the creep area and spent more time in proximity to the sow (p < 0.05). Within the commercial validation study, no impacts were seen on piglet production measures (p > 0.05). Largely the use of an SOA within the farrowing house did not impact the sow or her piglets in either the intensive study or commercial validation study. Based on these current results, the use of SOA within the farrowing house is not supported.
The Emergence of Multidrug-Resistant Helicobacter pylori in Southeast Asia: A Systematic Review on the Trends and Intervention Strategies Using Antimicrobial Peptides
Authors: Sukri Asif , Lopes Bruno S. , Hanafiah Alfizah
Published: September 2021

The emergence of multidrug-resistant H. pylori poses a public healthcare threat, particularly in low- and middle-income countries. Recently, the World Health Organization has classified clarithromycin-resistant H. pylori as high priority in the research and discovery of novel antibiotics. This study was aimed to systematically review the prevalence of primary antibiotic resistance in H. pylori in Southeast Asian countries (SEAC) and to review current studies of antimicrobial peptides against H. pylori. We systematically searched through electronic databases of studies conducted on antimicrobial resistance of H. pylori in SEA countries. Furthermore, we searched articles that conducted studies on antimicrobial peptides, naturally occurring host’s defense molecules, against H. pylori. After a series of screening processes, 15 studies were included in our systematic review. Our analysis revealed that primary resistance of H. pylori to metronidazole, clarithromycin, and levofloxacin were high in SEAC, although the primary resistance to amoxicillin and tetracycline remains low. Multidrug-resistant H. pylori are emerging in SE Asian countries. The antimicrobial peptides show promising antibacterial and antibiofilm activity against drug-resistant H. pylori. The research and discovery of antimicrobial peptides against H. pylori in SEAC will help in limiting the spread of antimicrobial resistance of H. pylori.