bluuebunny/crossref_metadata_2025 huggingface dataset:
Aggregated Distribution as an Explanation for the Paradox of Plankton and Collective Animal Behavior
Authors: Falgueras-Cano Javier , Falgueras-Cano Juan Antonio , Moya Andrés
Published: October 2022

This work analyzes the evolutionary consequences of different aggregation levels of species distribution with an Evolutionary Cellular Automaton (ECA). We have found that in habitats with the same carrying capacity, aggregated distributions preserve smaller populations than do uniform distributions, i.e., they are less efficient. Nonetheless, we have also found that aggregated distributions, among other factors, can help the evolutionary stability of some biological interactions, such as predator–prey interactions, despite their granting less individual fitness. Besides, the competitive exclusion principle does not usually stand in populations with aggregated distribution. We have applied ECA to study the effects of aggregated distribution in two notorious cases: in the so-called paradox of the plankton and in gregarious animals. In doing so, we intend to ratify long-established ecological knowledge explaining these phenomena from a new perspective. In the first case, due to aggregate distribution, large aggregations of digital organisms mimicking very abundant planktonic species, leave large patches or oceanic areas free for other less competitive organisms, which mimic rare species, to prosper. In this case, we can see how effects, such as ecological drift and the small portion, act simultaneously. In the second case of aggregation, the aggregate distribution of gregarious animals could be explained under specialized predator–prey interactions and interdemic competition. Thus, digital organisms that imitate predators reduce the competitive capacity of their prey, destabilizing their competitiveness against other species. The specialized predator also goes extinct if the prey goes extinct by natural selection. Predators that have an aggregate distribution compensate the prey and thus avoid exclusion. This way there are more predator-free patches in which the prey can prosper. However, by granting greater colonization capacity to its prey, the predator loses competitiveness. Therefore, it is a multilevel selection event in which group adaptation grows to the detriment of the predator as an individual.
Paramutation-like Epigenetic Conversion by piRNA at the Telomere of Drosophila virilis
Authors: Dorador Ana P. , Dalikova Martina , Cerbin Stefan , Stillman Chris M. , Zych Molly G. , Hawley R. Scott , Miller Danny E. , Ray David A. , Funikov Sergei Y. , Evgen’ev Michael B. , Blumenstiel Justin P.
Published: October 2022

First discovered in maize, paramutation is a phenomenon in which one allele can trigger an epigenetic conversion of an alternate allele. This conversion causes a genetically heterozygous individual to transmit alleles that are functionally the same, in apparent violation of Mendelian segregation. Studies over the past several decades have revealed a strong connection between mechanisms of genome defense against transposable elements by small RNA and the phenomenon of paramutation. For example, a system of paramutation in Drosophila melanogaster has been shown to be mediated by piRNAs, whose primary function is to silence transposable elements in the germline. In this paper, we characterize a second system of piRNA-mediated paramutation-like behavior at the telomere of Drosophila virilis. In Drosophila, telomeres are maintained by arrays of retrotransposons that are regulated by piRNAs. As a result, the telomere and sub-telomeric regions of the chromosome have unique regulatory and chromatin properties. Previous studies have shown that maternally deposited piRNAs derived from a sub-telomeric piRNA cluster can silence the sub-telomeric center divider gene of Drosophila virilis in trans. In this paper, we show that this silencing can also be maintained in the absence of the original silencing allele in a subsequent generation. The precise mechanism of this paramutation-like behavior may be explained by either the production of retrotransposon piRNAs that differ across strains or structural differences in the telomere. Altogether, these results show that the capacity for piRNAs to mediate paramutation in trans may depend on the local chromatin environment and proximity to the uniquely structured telomere regulated by piRNAs. This system promises to provide significant insights into the mechanisms of paramutation.
Synbiotics and Their Antioxidant Properties, Mechanisms, and Benefits on Human and Animal Health: A Narrative Review
Authors: Mounir Majid , Ibijbijen Amal , Farih Kawtar , Rabetafika Holy N. , Razafindralambo Hary L.
Published: October 2022

Antioxidants are often associated with a variety of anti-aging compounds that can ensure human and animal health longevity. Foods and diet supplements from animals and plants are the common exogenous sources of antioxidants. However, microbial-based products, including probiotics and their derivatives, have been recognized for their antioxidant properties through numerous studies and clinical trials. While the number of publications on probiotic antioxidant capacities and action mechanisms is expanding, that of synbiotics combining probiotics with prebiotics is still emerging. Here, the antioxidant metabolites and properties of synbiotics, their modes of action, and their different effects on human and animal health are reviewed and discussed. Synbiotics can generate almost unlimited possibilities of antioxidant compounds, which may have superior performance compared to those of their components through additive or complementary effects, and especially by synergistic actions. Either combined with antioxidant prebiotics or not, probiotics can convert these substrates to generate antioxidant compounds with superior activities. Such synbiotic-based new routes for supplying natural antioxidants appear relevant and promising in human and animal health prevention and treatment. A better understanding of various component interactions within synbiotics is key to generating a higher quality, quantity, and bioavailability of antioxidants from these biotic sources.
Aggravated Gut Microbiota and Metabolomic Imbalances Are Associated with Hypertension Patients Comorbid with Atrial Fibrillation
Authors: Fang Chen , Zuo Kun , Fu Yuan , Zhu Xiaoming , Li Jing , Zhong Jiuchang , Xu Li , Yang Xinchun
Published: October 2022

Disordered gut microbiota (GM) as the co-contributor of atrial fibrillation (AF) and hypertension (HTN) might be associated with AF risk in HTN. This study aimed to explore the altered GM community and metabolic patterns between 27 HTN patients with AF (HTN-AF) and 27 non-AF HTN patients through fecal metagenomic and serum metabolomic analysis. Compared to non-AF HTN patients, significant microbial alterations (p = 0.004), including increased microbial diversity (p < 0.05), shifted enterotype dominated by Prevotella to Bacteroides, and abundant disease-linked genera Ruminococcus, Streptococcus, Veillonella, Dorea, and Enterococcus, were observed in HTN-AF patients. A species-based random forest prediction model was associated with the risk of AF occurrence in HTN patients. Furthermore, GM metabolic profiles dramatically differed between HTN and HTN-AF patients, especially the imbalance of saturated and unsaturated fatty acids. In HTN-AF patients, circulating palmitic acid and arachidonic acid levels were significantly elevated, while the levels of tetracosahexaenoic acid, oleic acid, linoleic acid, and stearic acid were decreased (p < 0.001, VIP > 1), mediating 85.99% of gut microbial indirect effects on AF (p < 0.001). Thus, our findings preliminarily indicated that exacerbated dysbiosis of GM and relevant metabolites was associated with high AF susceptibility and might be a potential target for AF prediction and prevention in HTN.
Andrographolide Inhibits Corneal Fibroblast to Myofibroblast Differentiation In Vitro
Authors: Rozo Vanessa , Quan Melinda , Aung Theint , Kang Jennifer , Thomasy Sara M. , Leonard Brian C.
Published: October 2022

Corneal opacification due to fibrosis is a leading cause of blindness worldwide. Fibrosis occurs from many causes including trauma, photorefractive surgery, microbial keratitis (infection of the cornea), and chemical burns, yet there is a paucity of therapeutics to prevent or treat corneal fibrosis. This study aimed to determine if andrographolide, a labdane diterpenoid found in Andrographis paniculate, has anti-fibrotic properties. Furthermore, we evaluated if andrographolide could prevent the differentiation of fibroblasts to myofibroblasts in vitro, given that the transforming growth factor beta-1(TGF-β1) stimulated persistence of myofibroblasts in the cornea is a primary component of fibrosis. We demonstrated that andrographolide inhibited the upregulation of alpha smooth muscle actin (αSMA) mRNA and protein in rabbit corneal fibroblasts (RCFs), thus, demonstrating a reduction in the transdifferentiation of myofibroblasts. Immunofluorescent staining of TGF-β1-stimulated RCFs confirmed a dose-dependent decrease in αSMA expression when treated with andrographolide. Additionally, andrographolide was well tolerated in vivo and had no impact on corneal epithelialization in a rat debridement model. These data support future studies investigating the use of andrographolide as an anti-fibrotic in corneal wound healing.
Exosomal miR92a Promotes Cytarabine Resistance in Myelodysplastic Syndromes by Activating Wnt/β-catenin Signal Pathway
Authors: Li Hongjiao , Xie Chenglian , Lu Yurong , Chang Kaijing , Guan Feng , Li Xiang
Published: October 2022

Cytarabine (Ara-C) has been one of the frontline therapies for clonal hematopoietic stem cell disorders, such as myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML), but Ara-C resistance often occurs and leads to treatment failure. Exosomal microRNAs (miRNAs, miRs) as small noncoding RNA that play important roles in post-transcriptional gene regulation, can be delivered into recipient cells by exosomes and regulate target genes’ expression. miR92a has been reported to be dysregulated in many cancers, including MDS and AML. However, the effects of exosomal miR92a in hematologic malignancies have not been fully investigated. In this study, qualitative analysis showed the significantly enhanced expression of exosomal miR92a in MDS/AML plasma. Subsequent functional assays indicated that exosomal miR92a can be transported and downregulate PTEN in recipient cells and, furthermore, activate the Wnt/β-catenin signaling pathway and interfere with the Ara-C resistance of receipt MDS/AML cells in vitro and in vivo. Altogether, our findings offer novel insights into plasma exosomal miR92a participating in Ara-C resistance in MDS/AML and we propose miR92a as a potential therapeutic target for MDS/AML.
Recent Advances in the Use of CoPc-MWCNTs Nanocomposites as Electrochemical Sensing Materials
Authors: Balogun Sheriff A. , Fayemi Omolola E.
Published: October 2022

Cobalt phthalocyanine multiwalled carbon nanotubes (CoPc-MWCNTs), a nanocomposite, are extraordinary electrochemical sensing materials. This material has attracted growing interest owing to its unique physicochemical properties. Notably, the metal at the center of the metal phthalocyanine structure offers an enhanced redox-active behavior used to design solid electrodes for determining varieties of analytes. This review extensively discusses current developments in CoPc-MWCNTs nanocomposites as potential materials for electrochemical sensors, along with their different fabrication methods, modifying electrodes, and the detected analytes. The advantages of CoPc-MWCNTs nanocomposite as sensing material and its future perspectives are carefully reviewed and discussed.
Monk Parakeet’s (Myiopsitta monachus) Ecological Parameters after Five Decades of Invasion in Santiago Metropolis, Chile
Authors: Briceño Cristóbal , Larraechea Matilde , Alvarado Sergio
Published: October 2022

Monk Parakeets are considered one of the most invasive bird species given its unique capacity among psittacines to build their own communal nests. Originally introduced as pets in houses from where they escaped or were released, they are currently considered invasive in more than 20 countries worldwide. This is the case in Chile, where Monk Parakeets were introduced during the 1970s. Between 2016 and 2019 we searched Monk Parakeets’ nests structures in the Santiago metropolis region. We identified 1458 Monk Parakeets’ communal nests on 546 trees belonging to 34 tree species. Ninety-one percent of the occupied trees were also introduced. Paraná pine and cedar of Lebanon were the tree species with highest abundance of nests, averaging more than four nests/tree/species, with 23 and 18 maximum number of nests, respectively. Tasmanian blue gum and black locust were selected by parakeets more often than expected, based on availability. From all trees, 24.6% denoted health problems and 47.3% were pruned. The average nest height was 14.2 m and nests were observed mainly in secondary branches (59.3%). The occupancy rate was 89.7% and was associated to nest height and type of branch. During two reproductive seasons we quantified eggs and nestlings in chambers averaging 4.5 and 4.2, respectively. We provide a rough population size estimate and the characteristics of Monk Parakeets nest and tree selectivity, aiming to characterize several decades of a neglected urban invasion to warrant strategies for improved management measures.
microRNA Biology on Brain Development and Neuroimaging Approach
Authors: Tsujimura Keita , Shiohama Tadashi , Takahashi Emi
Published: October 2022

Proper brain development requires the precise coordination and orchestration of various molecular and cellular processes and dysregulation of these processes can lead to neurological diseases. In the past decades, post-transcriptional regulation of gene expression has been shown to contribute to various aspects of brain development and function in the central nervous system. MicroRNAs (miRNAs), short non-coding RNAs, are emerging as crucial players in post-transcriptional gene regulation in a variety of tissues, such as the nervous system. In recent years, miRNAs have been implicated in multiple aspects of brain development, including neurogenesis, migration, axon and dendrite formation, and synaptogenesis. Moreover, altered expression and dysregulation of miRNAs have been linked to neurodevelopmental and psychiatric disorders. Magnetic resonance imaging (MRI) is a powerful imaging technology to obtain high-quality, detailed structural and functional information from the brains of human and animal models in a non-invasive manner. Because the spatial expression patterns of miRNAs in the brain, unlike those of DNA and RNA, remain largely unknown, a whole-brain imaging approach using MRI may be useful in revealing biological and pathological information about the brain affected by miRNAs. In this review, we highlight recent advancements in the research of miRNA-mediated modulation of neuronal processes that are important for brain development and their involvement in disease pathogenesis. Also, we overview each MRI technique, and its technological considerations, and discuss the applications of MRI techniques in miRNA research. This review aims to link miRNA biological study with MRI analytical technology and deepen our understanding of how miRNAs impact brain development and pathology of neurological diseases.
Seeking Overlapping Neuroanatomical Alterations between Dyslexia and Attention-Deficit/Hyperactivity Disorder: A Meta-Analytic Replication Study
Authors: Liloia Donato , Crocetta Annachiara , Cauda Franco , Duca Sergio , Costa Tommaso , Manuello Jordi
Published: October 2022

The present work is a replication article based on the paper “Are there shared neural correlates between dyslexia and ADHD? A meta-analysis of voxel-based morphometry studies” by McGrath and Stoodley (2019). In the original research, the authors used activation likelihood estimation (ALE), a technique to perform coordinate-based meta-analysis (CBMA), to investigate the existence of brain regions undergoing gray matter alteration in association with both attention-deficit/hyper-activity disorder (ADHD) and dyslexia. Here, the same voxel-based morphometry dataset was analyzed, while using the permutation-subject images version of signed differential mapping (PSI-SDM) in place of ALE. Overall, the replication converged with the original paper in showing a limited overlap between the two conditions. In particular, no significant effect was found for dyslexia, therefore precluding any form of comparison between the two disorders. The possible influences of biological sex, age, and medication status were also ruled out. Our findings are in line with literature about gray matter alteration associated with ADHD and dyslexia, often showing conflicting results. Therefore, although neuropsychological and clinical evidence suggest some convergence between ADHD and dyslexia, more future research is sorely needed to reach a consensus on the neuroimaging domain in terms of patterns of gray matter alteration.
Multisensory Integration in Caenorhabditis elegans in Comparison to Mammals
Authors: Yu Yanxun V. , Xue Weikang , Chen Yuanhua
Published: October 2022

Multisensory integration refers to sensory inputs from different sensory modalities being processed simultaneously to produce a unitary output. Surrounded by stimuli from multiple modalities, animals utilize multisensory integration to form a coherent and robust representation of the complex environment. Even though multisensory integration is fundamentally essential for animal life, our understanding of the underlying mechanisms, especially at the molecular, synaptic and circuit levels, remains poorly understood. The study of sensory perception in Caenorhabditis elegans has begun to fill this gap. We have gained a considerable amount of insight into the general principles of sensory neurobiology owing to C. elegans’ highly sensitive perceptions, relatively simple nervous system, ample genetic tools and completely mapped neural connectome. Many interesting paradigms of multisensory integration have been characterized in C. elegans, for which input convergence occurs at the sensory neuron or the interneuron level. In this narrative review, we describe some representative cases of multisensory integration in C. elegans, summarize the underlying mechanisms and compare them with those in mammalian systems. Despite the differences, we believe C. elegans is able to provide unique insights into how processing and integrating multisensory inputs can generate flexible and adaptive behaviors. With the emergence of whole brain imaging, the ability of C. elegans to monitor nearly the entire nervous system may be crucial for understanding the function of the brain as a whole.
Clinical Utility of Eye Tracking in Assessing Distractibility in Children with Neurological Disorders or ADHD: A Cross-Sectional Study
Authors: Sweere Dirk J. J. , Pel Johan J. M. , Kooiker Marlou J. G. , van Dijk Johannes P. , van Gemert Elizabeth J. J. M. , Hurks Petra P. M. , Klinkenberg Sylvia , Vermeulen R. Jeroen , Hendriksen Jos G. M.
Published: October 2022

This study aims to investigate distractibility quantified by recording and analyzing eye movements during task-irrelevant distraction in children with and without ADHD and in children with and without neurological disorders. Gaze behavior data and press latencies of 141 participants aged 6–17 that were collected during a computerized distraction paradigm with task-irrelevant stimuli (IDistrack) were analyzed. Children using attention-regulating medication were excluded from participation. Data were analyzed for subgroups that were formed based on the presence of neurological disorders and the presence of ADHD separately. Participants with ADHD and participants with neurological disorders spent less time fixating on the target stimuli compared to their peers without ADHD (p = 0.025) or their peers without neurological disorders (p < 0.001). Participants with and without ADHD had equal press latencies (p = 0.79). Participants with neurological disorders had a greater press latency compared to their typically developing peers (p < 0.001). Target fixation duration shows a significant association with parent-reported attention problems (r = −0.39, p < 0.001). We conclude that eye tracking during a distraction task reveals potentially valid clinical information that may contribute to the assessment of dysfunctional attentional processes. Further research on the validity and reliability of this paradigm is recommended.
Association between Cerebral Coordination Functions and Clinical Outcomes of Alzheimer’s Dementia
Authors: Yang Yuan-Han , Lee Ying-Han , Yen Chen-Wen , Huang Ling-Chun , Chang Yang-Pei , Chien Ching-Fang
Published: October 2022

Background: Alzheimer’s dementia (AD) is a degenerative disease that impairs cognitive function, initially, and then motor or other function, eventually. Motor coordination function impairment usually accompanies cognition impairment but it is seldom examined whether it can reflect the clinical outcomes of AD. Methods: 113 clinically diagnosed AD patients with a mean age of 78.9 ± 6.9 years underwent an annual neuropsychological assessment using the Mini-Mental State Examination (MMSE), the Cognitive Abilities Screening Instrument (CASI), the Sum of Boxes of Clinical Dementia Rating (CDR-SB), and the CDR. The cerebral coordination function was evaluated through correlations among 15 joints with a kinetic depth sensor annually. An intra-individual comparison of both cognitive and motor coordination functions was performed to examine their correlations. Results: The changes in coordination function in the lower limbs can significantly reflect the clinical outcomes, MMSE (p < 0.001), CASI (p = 0.006), CDR (p < 0.001), and CDR-SB (p < 0.001), but the changes in upper limbs can only reflect the clinical outcome in CDR (p < 0.001). Conclusions: The use of a kinetic depth sensor to determine the coordination between joints, especially in lower limbs, can significantly reflect the global functional and cognitive outcomes in AD. Such evaluations could be another biomarker used to evaluate non-cognitive outcomes in AD for clinical and research purposes.
NGFR Gene and Single Nucleotide Polymorphisms, rs2072446 and rs11466162, Playing Roles in Psychiatric Disorders
Authors: Zhao Longyou , Hou Binyin , Ji Lei , Ren Decheng , Yuan Fan , Liu Liangjie , Bi Yan , Yang Fengping , Yu Shunying , Yi Zhenghui , Liu Chuanxin , Bai Bo , Yu Tao , Cai Changqun , He Lin , He Guang , Shi Yi , Li Xingwang , Wu Shaochang
Published: October 2022

Psychiatric disorders are a class of complex disorders characterized by brain dysfunction with varying degrees of impairment in cognition, emotion, consciousness and behavior, which has become a serious public health issue. The NGFR gene encodes the p75 neurotrophin receptor, which regulates neuronal growth, survival and plasticity, and was reported to be associated with depression, schizophrenia and antidepressant efficacy in human patient and animal studies. In this study, we investigated its association with schizophrenia and major depression and its role in the behavioral phenotype of adult mice. Four NGFR SNPs were detected based on a study among 1010 schizophrenia patients, 610 patients with major depressive disorders (MDD) and 1034 normal controls, respectively. We then knocked down the expression of NGFR protein in the hippocampal dentate gyrus of the mouse brain by injection of shRNA lentivirus to further investigate its behavioral effect in mice. We found significant associations of s2072446 and rs11466162 for schizophrenia. Ngfr knockdown mice showed social and behavioral abnormalities, suggesting that it is linked to the etiology of neuropsychiatric disorders. We found significant associations between NGFR and schizophrenia and that Ngfr may contribute to the social behavior of adult mice in the functional study, which provided meaningful clues to the pathogenesis of psychiatric disorders.
Brain Network Research of Skilled Shooters in the Shooting Preparation Stage under the Condition of Limited Sensory Function
Authors: Gu Feng , Gong Anmin , Qu Yi , Lu Ling , Shi Qidi , Fu Yunfa
Published: October 2022

Shooting is a sport dominated by psychological factors. Hence, disturbing the shooter’s sensory function during aiming will seriously affect his psychological state and shooting performance. Electroencephalograph (EEG) measurements of 30 skilled marksmen in the shooting preparation stage under noisy disturbance, weak light, and normal conditions were recorded. Therefore, the differences in neural mechanisms in the shooter’s brain during shooting aiming in different disturbance conditions were explored using an analytical approach that employs functional connectivity and brain network analysis based on graph theory. The relationship between these brain network characteristics and shooting performance was also compared. The results showed that (1) the average connection strength in the beta frequency band and connection intensity in the left and right temporal lobes of the shooters under noise disturbance were significantly higher than those under the other two conditions, and their brain networks also showed a higher global and local efficiency. In addition, (2) the functional connection intensity in the occipital region of the beta band was higher than that in the normal condition in the weak-light condition. The information interaction in the left parietal region also increased continually during the shooting process. (3) Furthermore, the shooters’ eigenvector centrality in the temporal and occipital regions with limited sensory function in the two conditions was lower than those in the normal condition. These findings suggest that noise disturbance activates the arousal level of the shooter’s brain and enhances the information processing efficiency of the brain network; however, it increases the mental workload. In weak-light conditions, shooters focus more on visual information processing during aiming and strengthen the inhibition of functions in the brain regions unrelated to shooting behavior. Audiovisual disturbance renders the cortical regions equivalent to the audiovisual perception function in the shooter’s brain less important in the entire brain network than in the normal condition. Therefore, these findings reveal the effect of audiovisual disturbance on the functional network of the cortex in the shooting preparation stage and provide a theoretical basis for further understanding the neural mechanism of the shooting process under sensory disturbances.
Intra-Arrest Therapeutic Hypothermia and Neurologic Outcome in Patients Admitted after Out-of-Hospital Cardiac Arrest: A Post Hoc Analysis of the Princess Trial
Authors: MACCHINI Elisabetta , DILLENBECK Emelie , JONSSON Martin , ANNONI Filippo , FORSBERG Sune , HOLLENBERG Jacob , TRUHLAR Anatolij , SVENSSON Leif , NORDBERG Per , TACCONE Fabio Silvio
Published: October 2022

Background: Despite promising results, the role of intra-arrest hypothermia in out-of-hospital cardiac arrest (OHCA) remains controversial. The aim of this study was to assess the effects of trans-nasal evaporative cooling (TNEC) during resuscitation on neurological recovery in OHCA patients admitted alive to the hospital. Methods: A post hoc analysis of the PRINCESS trial, including only patients admitted alive to the hospital, either assigned to TNEC or standard of care during resuscitation. The primary endpoint was favorable neurological outcome (FO) defined as a Cerebral Performance Category (CPC) of 1–2 at 90 days. The secondary outcomes were overall survival at 90 days and CPC 1 at 90 days. Subgroup analyses were performed according to the initial cardiac rhythm. Results: A total of 149 patients in the TNEC and 142 in the control group were included. The number of patients with CPC 1–2 at 90 days was 56/149 (37.6%) in the intervention group and 45/142 (31.7%) in the control group (p = 0.29). Survival and CPC 1 at 90 days was observed in 60/149 patients (40.3%) vs. 52/142 (36.6%; p = 0.09) and 50/149 (33.6%) vs. 35/142 (24.6%; p = 0.11) in the two groups. In the subgroup of patients with an initial shockable rhythm, the number of patients with CPC 1 at 90 days was 45/83 (54.2%) in the intervention group and 27/78 (34.6%) in the control group (p = 0.01). Conclusions: In this post hoc analysis of admitted OHCA patients, no statistically significant benefits of TNEC on neurological outcome at 90 days was found. In patients with initial shockable rhythm, TNEC was associated with increased full neurological recovery.
Experimental and Numerical Study on the Flexural Behavior of Cold-Formed Steel Multi-Limb Built-Up Section Beams
Authors: Deng Feiyun , He Yulong , Deng Lu , Zhong Wenjie
Published: October 2022

Cold-formed steel (CFS) is a typical green building material with the merits of being low-cost, lightweight, high-strength, and recyclable. CFS built-up section beams are widely used in CFS frames owing to their outstanding mechanical properties. However, a simplified and accurate method for calculating the flexural moment capacity of multi-limb built-up beams is missing in specifications. In this study, the flexural behaviors of CFS four-limb built-up beams with closed and open sections are investigated via experiments and finite element (FE) modeling. Firstly, the flexural moment capacities and failure modes of the beams are obtained by four-point bending experiments. The ultimate load capacity of the new open section beam is found to be higher than that of the closed section beam, and the failure mode is local buckling of the web and upper flange. Then, the FE models validated by the tests are developed to conduct an extensive parametric study. Numerical results show that the flexural moment capacity increases with the thickness and web depth. Finally, a simplified calculation method for the flexural moment capacities of the closed and open section beams is proposed by considering the reduction factor of gross section modulus of the built-up section.
Bending Performance of Precast Ceramsite-Concrete-Insulated Sandwich Panel with Stainless Steel Shear Connectors
Authors: Chen Yingjie , Kang Chaofeng , Wu Yunfeng , Qian Zhenxiao
Published: October 2022

With the continuous improvement of building energy-conservation requirements, both traditional concrete external insulation and internal insulation have been unable to meet energy-saving needs. In order to meet the demands of building energy-saving in the new era, new precast concrete external-wall-insulation technology should be developed. In this study, a bending static test and numerical simulation were carried out to evaluate the influence of the thickness of inner concrete wythe and insulation and the length of plate-type shear connectors on the cracking condition, bearing capacity and composite degree of a precast ceramsite-concrete-insulated sandwich panel (PCCISP) under the outside-plane load. The results show that the failure modes of four precast ceramsite-concrete-insulated sandwich panels were all ductile failure of the concrete flexural members. The ultimate bearing capacity of the PCCISP decreased with the decrease in the thickness of the inner concrete wythe. Reducing the thickness of insulation had no significant influence on the ultimate bearing capacity. When the thickness of insulation was reduced by 30%, the composite degree of rigidity and bearing capacity of the PCCISP were increased by 8.85% and 2.67%, respectively. Increasing the length of the plate-type shear connector slightly increased the ultimate bearing capacity, but it had no obvious influence on the rigidity and bearing capacity composite degree.
Analyzing Green Construction Development Barriers by a Hybrid Decision-Making Method Based on DEMATEL and the ANP
Authors: Kamranfar Saeed , Azimi Yousef , Gheibi Mohammad , Fathollahi-Fard Amir M. , Hajiaghaei-Keshteli Mostafa
Published: October 2022

There is a great deal of interest in analyzing construction development barriers to identify and rank them based on sustainability criteria and have less environmental pollution. Due to the importance of construction projects in developing countries such as Iran, this study implements a green construction development paradigm to identify and rank barriers for a case study in Tehran, Iran. The main novelty of this paper is the development of a new decision-making method using the DEMATEL and Delphi techniques and the ANP. In this regard, first of all, data collection is performed through a literature review and survey studies using questionnaires, interviews, and observations. The applied method for experts’ agreement was integrated through brainstorming and the classical Delphi method. By analyzing different economic, environmental, cultural, and social criteria using a hybrid decision-making framework, the results show that the main economic barrier with a weight of 0.2607 is ranked first, while the main feature of economic assessment is connected to the risk of investment. The cultural and social barriers, with a weight of 0.2258, ranked second, and the managerial barrier, with a weight of 0.2052, ranked third. In the social and managerial aspects, the main barriers were related to looking at green construction as luxurious and the uncertainty of green construction performance due to the climate and texture of the local area, respectively. According to the findings and results, the proposed barriers and sub-barriers in this study can be used to develop and create planning at the strategic level for the development of green construction for our case study in Tehran, Iran. With a concentration on the outcomes of the present research, the sustainable green building framework can be implemented by the application of a prioritized knowledge management concept.
Acid Adaptation Promotes TRPC1 Plasma Membrane Localization Leading to Pancreatic Ductal Adenocarcinoma Cell Proliferation and Migration through Ca2+ Entry and Interaction with PI3K/CaM
Authors: Schnipper Julie , Kouba Sana , Hague Frédéric , Girault Alban , Telliez Marie-Sophie , Guénin Stéphanie , Ahidouch Ahmed , Pedersen Stine Falsig , Ouadid-Ahidouch Halima
Published: October 2022

Pancreatic ductal adenocarcinoma (PDAC) remains one of the most lethal malignancies, with a low overall survival rate of less than 10% and limited therapeutic options. Fluctuations in tumor microenvironment pH are a hallmark of PDAC development and progression. Many ion channels are bona fide cellular sensors of changes in pH. Yet, the interplay between the acidic tumor microenvironment and ion channel regulation in PDAC is poorly understood. In this study, we show that acid adaption increases PANC-1 cell migration but attenuates proliferation and spheroid growth, which are restored upon recovery. Moreover, acid adaptation and recovery conditions favor the plasma membrane localization of the pH-sensitive calcium (Ca2+) channel transient receptor potential C1 (TRPC1), TRPC1-mediated Ca2+ influx, channel interaction with the PI3K p85α subunit and calmodulin (CaM), and AKT and ERK1/2 activation. Knockdown (KD) of TRPC1 suppresses cell migration, proliferation, and spheroid growth, notably in acid-recovered cells. KD of TRPC1 causes the accumulation of cells in G0/G1 and G2/M phases, along with reduced expression of CDK6, −2, and −1, and cyclin A, and increased expression of p21CIP1. TRPC1 silencing decreases the basal Ca2+ influx in acid-adapted and -recovered cells, but not in normal pH conditions, and Ca2+ chelation reduces cell migration and proliferation solely in acid adaptation and recovery conditions. In conclusion, acid adaptation and recovery reinforce the involvement of TRPC1 in migration, proliferation, and cell cycle progression by permitting Ca2+ entry and forming a complex with the PI3K p85α subunit and CaM.
Time Trend and Association of Early-Onset Colorectal Cancer with Diverticular Disease in the United States: 2010–2021
Authors: Wang Lindsey , Xu Rong , Kaelber David C. , Berger Nathan A.
Published: October 2022

Purpose: To examine time trends of incidence rates of EOCRC from 2010 to 2021 among patients with and without diverticular disease and to examine whether diverticular disease is associated with increased risk of EOCRC. Methods: This is a retrospective cohort study of 46,179,351 young adults aged 20–49, including 298,117 with diverticular disease. We examined yearly incidence rate of first diagnosis of EOCRC from 2010 through 2021 among patients with and without diverticular disease. The 5-year risk of EOCRC among patients with pre-existing diverticular disease was compared to propensity-matched patients without diverticular disease and EOCRC and odds ratio (OR) and 95% confidence interval (CI) were calculated. Results: The yearly incidence rate of new diagnosis of EOCRC (measured as new cases per 100,000 people per year) in young adults with pre-existing diverticular disease increased from 100 in 2010 to 402 in 2021, 4–6 times higher than in those without diverticular disease (24 in 2010 to 77 in 2021) (p < 0.001). Patients with diverticular disease were at higher risk for EOCRC than those without (OR: 1.76, 95% CI: 1.40–2.32). Conclusion: The incidence of EOCRC continuously increased from 2010 through 2021 in patients with and without diverticular disease and was 4–6 times higher among patients with diverticular disease. Patients with pre-existing diverticular disease were at a significantly increased risk for EOCRC.
Evaluation of the Cellular Dissociation Grading, Based on Tumor Budding and Cell Nest Size, in Squamous Cell Carcinoma of the Penis
Authors: Derani Hayel , Becker Anne-Sophie , Hakenberg Oliver , Erbersdobler Andreas
Published: October 2022

The “Cellular Dissociation Grade” (CDG) is based on tumor cell budding and cell nest size. Many studies have examined the CDG in squamous cell carcinomas of other organs such as the lungs, oral cavity, pharynx, larynx, cervix and esophagus. In this study, the CDG was examined in 109 cases of invasive penile squamous cell carcinoma that were treated at the University Medicine Rostock between 2014 and 2022. Furthermore, its correlation with the pathologic status of regional lymph nodes (pN) as the main prognostic factor was verified. Finally, cellular dissociation grading was compared with classic WHO grading. The results showed that pN in penile squamous cell carcinoma showed a highly significant association with the CDG and no statistically significant association with WHO grading. These results support the notion that cellular dissociation grading is an important prognostic factor for squamous cell carcinoma.
Targeting E2F Sensitizes Prostate Cancer Cells to Drug-Induced Replication Stress by Promoting Unscheduled CDK1 Activity
Authors: Hamidi Mohaddase , Eriz Ainhoa , Mitxelena Jone , Fernandez-Ares Larraitz , Aurrekoetxea Igor , Aspichueta Patricia , Iglesias-Ara Ainhoa , Zubiaga Ana M.
Published: October 2022

E2F1/E2F2 expression correlates with malignancy in prostate cancer (PCa), but its functional significance remains unresolved. To define the mechanisms governed by E2F in PCa, we analyzed the contribution of E2F target genes to the control of genome integrity, and the impact of modulating E2F activity on PCa progression. We show that silencing or inhibiting E2F1/E2F2 induces DNA damage during S phase and potentiates 5-FU-induced replication stress and cellular toxicity. Inhibition of E2F downregulates the expression of E2F targets involved in nucleotide biosynthesis (TK1, DCK, TYMS), whose expression is upregulated by 5-FU. However, their enzymatic products failed to rescue DNA damage of E2F1/E2F2 knockdown cells, suggesting additional mechanisms for E2F function. Interestingly, targeting E2F1/E2F2 in PCa cells reduced WEE1 expression and resulted in premature CDK1 activation during S phase. Inhibition of CDK1/CDK2 prevented DNA damage induced by E2F loss, suggesting that E2F1/E2F2 safeguard genome integrity by restraining CDK1/CDK2 activity. Importantly, combined inhibition of E2F and ATR boosted replication stress and dramatically reduced tumorigenic capacity of PCa cells in xenografts. Collectively, inhibition of E2F in combination with drugs targeting nucleotide biosynthesis or DNA repair is a promising strategy to provoke catastrophic levels of replication stress that could be applied to PCa treatment.
Amalgamated Titanium Oxide-Carbon Hollow Sphere/Nickel-Layered Double Hydroxide as an Efficient Photocatalyst for the Degradation of Methyl Orange
Authors: Al-Soihi Auhood S. , Alsulami Qana A. , Mostafa Mohamed Mokhtar M.
Published: October 2022

Investigating efficient and selective photocatalysts for water treatment can help address the energy crisis and numerous environmental issues associated with the use of current fossil fuels. As a shell, we used nickel-layered double hydroxide nanosheets on top of an anatase TiO2-carbon core to create an integrated photocatalyst. Materials were characterized using FTIR, XRD, SEM, HRTEM, and XPS methods for their physical-chemical properties. Using N2 adsorption/desorption at −196 °C, BET-surface area and pore structure were determined. Diffuse reflectance UV–vis was used to determine the photocatalysts band gap. For the TiO2-C/NiLDH amalgam, showed the lowest band gap (3.1 eV) with an exceptional ability to degrade methyl orange as an organic pollutant. Core–shell symmetry in the TiO2-C/NiLDH amalgam provides a larger surface area (72 m2/g) for interfacial interaction and a wider base for efficient charge transfer. In subsequent tests, this photocatalyst showed a remarkable level of stability and water treatment efficacy. That the TiO2-C/NiLDH amalgam can be used to alter solar energy and protect the environment has been demonstrated by these promising results.
Profound Effects of Dexamethasone on the Immunological State, Synthesis and Secretion Capacity of Human Testicular Peritubular Cells
Authors: Stepanov Youli Konstantinovitch , Speidel Jan Dominik , Herrmann Carola , Schmid Nina , Behr Rüdiger , Köhn Frank-Michael , Stöckl Jan Bernd , Pickl Ulrich , Trottmann Matthias , Fröhlich Thomas , Mayerhofer Artur , Welter Harald
Published: October 2022

The functions of human testicular peritubular cells (HTPCs), forming a small compartment located between the seminiferous epithelium and the interstitial areas of the testis, are not fully known but go beyond intratesticular sperm transport and include immunological roles. The expression of the glucocorticoid receptor (GR) indicates that they may be regulated by glucocorticoids (GCs). Herein, we studied the consequences of the GC dexamethasone (Dex) in cultured HTPCs, which serves as a unique window into the human testis. We examined changes in cytokines, mainly by qPCR and ELISA. A holistic mass-spectrometry-based proteome analysis of cellular and secreted proteins was also performed. Dex, used in a therapeutic concentration, decreased the transcript level of proinflammatory cytokines, e.g., IL6, IL8 and MCP1. An siRNA-mediated knockdown of GR reduced the actions on IL6. Changes in IL6 were confirmed by ELISA measurements. Of note, Dex also lowered GR levels. The proteomic results revealed strong responses after 24 h (31 significantly altered cellular proteins) and more pronounced ones after 72 h of Dex exposure (30 less abundant and 42 more abundant cellular proteins). Dex also altered the composition of the secretome (33 proteins decreased, 13 increased) after 72 h. Among the regulated proteins were extracellular matrix (ECM) and basement membrane components (e.g., FBLN2, COL1A2 and COL3A1), as well as PTX3 and StAR. These results pinpoint novel, profound effects of Dex in HTPCs. If transferrable to the human testis, changes specifically in ECM and the immunological state of the testis may occur in men upon treatment with Dex for medical reasons.
AIRE in Male Fertility: A New Hypothesis
Authors: Petrusová Jana , Manning Jasper , Filipp Dominik
Published: October 2022

Male infertility affects approximately 14% of all European men, of which ~44% are characterized as idiopathic. There is an urgency to identify the factors that affect male fertility. One such factor, Autoimmune Regulator (AIRE), a protein found in the thymus, has been studied in the context of central tolerance functioning as a nuclear transcription modulator, responsible for the expression of tissue-restricted antigens in specialized thymic cells that prevent autoimmunity. While its expression in the testes remains enigmatic, we recently observed that sterility in mice correlates with the absence of Aire in the testes, regardless of the deficient expression in medullary thymic epithelial cells or cells of the hematopoietic system. By assessing the Aire transcript levels, we discovered that Sertoli cells are the exclusive source of Aire in the testes, where it most likely plays a non-immune role, suggesting an unknown mechanism by which testicular Aire regulates fertility. Here, we discuss these results in the context of previous reports which have suggested that infertility observed in Aire deficient mice is of an autoimmune aetiology. We present an alternative point of view for the role of Aire in testes in respect to fertility altering the perspective of how Aire’s function in the testes is currently perceived.
Influence Factor Analysis and Prediction Model of End-Point Carbon Content Based on Artificial Neural Network in Electric Arc Furnace Steelmaking Process
Authors: Yang Lingzhi , Li Bo , Guo Yufeng , Wang Shuai , Xue Botao , Hu Shaoyan
Published: October 2022

In this manuscript, we consider the accuracy of end-point carbon content prediction affected by oxygen injection in multiple stages of electric arc furnace (EAF) melting process. Such a prediction would help to further evaluate process control strategies and optimize overall operation of the electric arc furnace. Principal component analysis (PCA) was used to normalize the 13 input variables affecting the endpoint carbon content. log-sigmoid and tan-sigmoid functions were used to verify the same sample, and it was found that the Mean squared error(MSE) of the model under logsig + logsig function was smaller, indicating that the model was more stable. At the same time, different hidden layer nodes were tried, and finally the structure of the model was determined as 13 × 10 × 8 × 1, and the activation function was logsig + logsig. Using historical smelting data to train and test the neural network model, the correlation coefficient (R) of the verified model is 0.7632, the model prediction is in the range of ±0.03%, the hit rate of the model is 64.5%, and the hit rate of the model is 42% in the range of ±0.02%. Combining the verification basis of the model with the metallurgical reaction principle of the EAF steelmaking process, a pretreatment method of phased input of total oxygen is proposed. The oxygen is divided into three stages, which are the oxygen consumption volume of 0–5 min, 5–30 min and more than 30 min and other variables are kept unchanged. The same neural network is used to train and verify the same data. After verification, the R of the oxygen staged model is 0.8274. The model prediction is in the range of ±0.03%, the hit rate of the model is 78.5%, and the hit rate of the model is 58% in the range of ±0.02%. Finally, an on-line carbon content prediction system based on artificial neural network model is developed and applied to actual production. Running results illustrated that the hit rate of end-point carbon content is 96.67%, 93.33% and 86.67%, respectively when the prediction errors are within ±0.05%, ±0.03% and ±0.01%, the improved neural network model can effectively predict the end-point carbon content, which provides a good basis for the carbon content at the end point of EAF steelmaking process.