bluuebunny/crossref_metadata_2025 huggingface dataset:
A new allele PEL9GG identified by genome-wide association study increases panicle elongation length in rice (Oryza sativa L.)
Authors: Dang Xiaojing , Jing Chunyu , Zhang Mengyuan , Li Xinru , Xu Qing , Hu Changmin , Li Yulong , Zhang Ying , Wang Dezheng , Hong Delin , Jiang Jianhua
Published: February 2023

Considering the male sterile line has the phenomenon of panicle enclosure, panicle elongation length (PEL) plays an important role in hybrid rice seed production. However, the molecular mechanism underlying this process is poorly understood. In this study, we investigated the PEL phenotypic values of 353 rice accessions across six environments, which shows abundant phenotypic variation. Combining the 1.3 million single-nucleotide polymorphisms, we performed a genome-wide association study on PEL. Three quantitative trait loci (QTL) qPEL4, qPEL6, and qPEL9 were identified as significantly associated with PEL, of which qPEL4 and qPEL6 were previously reported QTLs and qPEL9 was novel. One causal gene locus, PEL9, was identified and validated. The PEL of accessions carrying allele PEL9GG was significantly longer than that of those carrying allele PEL9TT. We also demonstrated that the outcrossing rate of female parents carrying allele PEL9GG increased by 14.81% compared with that of the isogenic line carrying allele PEL9TT in an F1 hybrid seed production field. The allele frequency of PEL9GG increased gradually with an increase in latitude in the Northern Hemisphere. Our results should facilitate the improvement of the PEL of the female parent of hybrid rice.
How do risks and benefits affect user’ privacy decisions? An event-related potential study on privacy calculus process
Authors: Fu Jialin , Zhang Jiaming , Li Xihang
Published: February 2023

Purpose

The purpose of this study is to examine how risks and benefits affect users’ privacy-related decision-making processes.

Design/methods/approach

This study collected and analyzed the neural activity processes of users’ privacy-related decisions when faced with personalized services with different risks and benefits through an ERP experiment that included 40 participants.

Findings/results

The findings show that users subconsciously categorize personalized services based on benefit; Privacy calculus affects privacy decision by influencing the allocation of cognitive resources for personalized service, and the scarcity of cognitive resources increases the degree of privacy disclosure; Emotional change in privacy decision is the result of many factors, not the result of privacy risk alone.

Originality/Discussion

This study provides a new perspective to explain the process of privacy decision-making, and a new approach to investigate the privacy paradox.
Examining missing pieces of the human resource (HR) attributions puzzle: The interplay between line manager beliefs, HR information and context
Authors: Vuorenmaa Hertta , Sumelius Jennie , Sanders Karin
Published: February 2023

While previous research acknowledges the importance of line manager interpretations of information coming from the HR department for explaining various employee attitudes and behaviors, less is known about the antecedents of these interpretations, also known as HR attributions. This paper provides a qualitative examination of the interplay between three key antecedents of HR attributions, namely, line managerbeliefsabout the HR department,informationfrom the HR department andcontext.Our analysis is based on 30 interviews with HR professionals and line managers in three units of one organization. Our findings suggest that differences in context have a strong impact on line manager beliefs about HR, influencing the way line managers see HR practices, processes and the role of the HR department, and consequently the way they interpret information coming from HR. Our analysis extends our understanding of the variability in line manager interpretations of HR information. Our results contribute to existing research on HRM strength and HR attributions by highlighting the importance of focusing not only on the consistency of the HR system, but also on individual line managers beliefs about HR, and the context in which HR processes take place.
Virtual mentalizing imagery therapy for Spanish language Latino family dementia caregivers: A feasibility and acceptability study
Authors: Ramirez-Gomez Liliana , Johnson Julene K. , Ritchie Christine , Meyer Ashley K. , Tan Emily , Madarasmi Saira , Gutierrez-Ramirez Paulina , Aldarondo-Hernández Cecilianna , Mischoulon David , Banerjee Sreya , Jain Felipe A.
Published: February 2023

Spanish speaking family caregivers of people living with dementia have limited supportive resources in Spanish. There are few validated, culturally acceptable virtual interventions for reducing these caregivers’ psychological distress. We investigated the feasibility of a Spanish language adaptation of a virtual Mentalizing Imagery Therapy (MIT) program, which provides guided imagery and mindfulness training to reduce depression, increase mentalizing, and promote well-being. 12 Spanish-speaking family dementia caregivers received a 4-week virtual MIT program. Follow-up was obtained post group and at 4 months post baseline assessment. Feasibility, acceptability, and satisfaction with MIT were assessed. The primary psychological outcome was depressive symptoms; secondary outcomes included caregiver burden, dispositional mindfulness, perceived stress, well-being, interpersonal support, and neurological quality of life. Statistical analysis was performed with mixed linear models. Caregivers were 52 ± 8 (mean ± SD) years of age. 60% had a high school education or less. Participation in weekly group meetings was 100%. Home practice was performed on average 4 ± 1 times per week [range 2–5]. Satisfaction with MIT reached 19 ± 2 of a possible 20 points. Reduction in depression from baseline was observed by week three (p = 0.01) and maintained at 4 month follow-up (p = 0.05). There were significant improvements in mindfulness post-group, and in caregiver burden and well-being at 4 months. MIT was successfully adapted for Latino Spanish language family dementia caregivers within a virtual group environment. MIT is feasible and acceptable and may help reduce depressive symptoms and improve subjective well-being. Larger, randomized controlled trials of MIT should determine durability of effects and validate efficacy in this population.
On offer to Ontario consumers three years after legalization: A profile of cannabis products, cannabinoid content, plant type, and prices
Authors: Tassone Felicia , Di Ciano Patricia , Liu Yuxin , Rueda Sergio
Published: February 2023

Introduction

Cannabis was legalized in Canada in October 2018, regulating the production, distribution, sale, and possession of dried cannabis and cannabis oils. Additional products were legalized 1 year later, including edibles, concentrates, and topicals, with new lines of commercial products coming to market. Ontario is the most populous province in Canada and has the largest cannabis market with the highest number of in-person retail stores and the most cannabis products available online. This study aims to create a profile of products available to consumers three years after legalization by summarizing types of products, THC and CBD potency, plant type, and prices of product sub-categories.

Methods

We extracted data from the website of the Ontario Cannabis Store (OCS)—the public agency overseeing the only online store and sole wholesaler to all authorized in-person stores—in the first quarter of 2022 (January 19–March 23). We used descriptive analyses to summarize the data. A total of 1,771 available products were mapped by route of administration into inhalation (smoking, vaping, and concentrates), ingestible (edibles, beverages, oils, and capsules) and topical.

Results

Most inhalation products included ≥20%/g THC (dried flower: 94%; cartridges: 96%; resin: 100%) while ingestible products had similar proportions of THC and CBD content. Indica-dominant products tend to be more prominent in inhalation products while sativa-dominant products tend to be more prominent in ingestible products. The average sale price of cannabis was 9.30 $/g for dried flower, 5.79 $/0.1g for cartridges, 54.82 $/g for resin, 3.21 $/unit for soft chews, 1.37 $/ml for drops, 1.52 $/unit for capsules, and 39.94 $/product for topicals.

Discussion

In summary, a wide variety of cannabis products were available to Ontarians for different routes of administration and provides numerous indica-dominant, sativa-dominant, and hybrid/blend options. The current market for inhalation products however is geared towards the commercialization of high-THC products.
The Chinese version of patient-doctor-relationship questionnaire (PDRQ-9): Factor structure, validation, and IRT psychometric analysis
Authors: Wang Yufei , Wu Aoxue , Jiang Yinan , Duan Yanping , Geng Wenqi , Wan Lin , Li Jiarui , Du Jianhua , Hu Jiaojiao , Jiang Jing , Shi Lili , Wei Jing
Published: February 2023

Objective

The patient-doctor relationship has been considered as a crucial concept in primary healthcare, while the medical reform launched by the Chinese government in 2009 has brought significant changes to the healthcare system, which made it urgent to introduce reliable measurement instruments for assessing today’s doctor-patient relationship in China. This study examined the psychometric properties of the Chinese version of the Patient-Doctor-Relationship Questionnaire-9 item (PDRQ-9) scale among general hospital inpatients in China.

Materials and methods

A total of 203 participants responded to the survey, of which 39 completed retest after 7 days. Factor analyses were used to test the construct validity of the scale. Convergent validity was evaluated by the correlation between PDRQ-9 and depressive symptoms measured using PHQ-9 (Patient Health Questionnaire Depression Scale-9 item). Both multidimensional item response theory (MIRT) and unidimensional item response theory (IRT) framework were used to estimate the parameters of each item.

Results

The two-factor model of relationship quality and treatment quality was supported (χ2/df = 1.494, GFI = 0.925, RMSEA = 0.071, RMR = 0.008, CFI = 0.985, NFI = 0.958, NNFI = 0.980, TLI = 0.980, IFI = 0.986). The PDRQ-9 and both subscales showed significant correlation with PHQ-9 (r = −0.196∼−0.309) and good internal consistency (Cronbach’s alpha = 0.865∼0.933). ANCOVA analysis adjusted with age revealed significant difference in PDRQ-9 ratings between patients with or without significant depressive symptoms (P = 0.019). The 7-day test-retest reliability of the scale was 0.730. The MIRT model of full scale and IRT models of both subscales showed high discrimination of all items (a = 2.46∼38.46), and the test information within the range of low-quality relationship was relatively high.

Conclusion

The Chinese version of PDRQ-9 is a valid and reliable rating scale, which can measure the doctor-patient relationship among Chinese patients.
The pharmacogenetics of the new-generation antipsychotics – A scoping review focused on patients with severe psychiatric disorders
Authors: Vasiliu Octavian
Published: February 2023

Exploring the possible correlations between gene variations and the clinical effects of the new-generation antipsychotics is considered essential in the framework of personalized medicine. It is expected that pharmacogenetic data will be useful for increasing the treatment efficacy, tolerability, therapeutic adherence, functional recovery, and quality of life in patients with severe psychiatric disorders (SPD). This scoping review investigated the available evidence about the pharmacokinetics, pharmacodynamics, and pharmacogenetics of five new-generation antipsychotics, i.e., cariprazine, brexpiprazole, aripiprazole, lumateperone, and pimavanserin. Based on the analysis of 25 primary and secondary sources and the review of these agents’ summaries of product characteristics, aripiprazole benefits from the most relevant data about the impact of gene variability on its pharmacokinetics and pharmacodynamics, with significant consequences on this antipsychotic’s efficacy and tolerability. The determination of the CYP2D6 metabolizer status is important when administering aripiprazole, either as monotherapy or associated with other pharmacological agents. Allelic variability in genes encoding dopamine D2, D3, and serotonin, 5HT2A, 5HT2C receptors, COMT, BDNF, and dopamine transporter DAT1 was also associated with different adverse events or variations in the clinical efficacy of aripiprazole. Brexpiprazole also benefits from specific recommendations regarding the CYP2D6 metabolizer status and the risks of associating this antipsychotic with strong/moderate CYP2D6 or CYP3A4 inhibitors. US Food and Drug Administration (FDA) and European Medicines Agency (EMA) recommendations about cariprazine refer to possible pharmacokinetic interactions with strong CYP3A4 inhibitors or inducers. Pharmacogenetic data about cariprazine is sparse, and relevant information regarding gene-drug interactions for lumateperone and pimavanserin is yet lacking. In conclusion, more studies are needed to detect the influence of gene variations on the pharmacokinetics and pharmacodynamics of new-generation antipsychotics. This type of research could increase the ability of clinicians to predict favorable responses to specific antipsychotics and to improve the tolerability of the treatment regimen in patients with SPD.
Willingness to pay for a COVID-19 vaccine and its associated determinants in Iran
Authors: Soofi Moslem , Kok Gerjo , Soltani Shahin , Kazemi-Karyani Ali , Najafi Farid , Karamimatin Behzad
Published: February 2023

Introduction

Understanding the individuals' willingness to pay (WTP) for the COVID-19 vaccine could help design policy interventions to control the COVID-19 pandemic. This study aimed to estimate the individuals' willingness to pay (WTP) for a COVID-19 vaccine and to identify its associated determinants.

Methods

A cross-sectional survey was conducted on 526 Iranian adults using a web-based questionnaire. A double-bounded contingent valuation approach was used to estimate WTP for the COVID-19 vaccine. The parameters of the model were estimated based on the maximum likelihood method.

Results

A considerable proportion of participants (90.87%) were willing to pay for a COVID-19 vaccine. Based on our discrete choice model, the estimated mean WTP for a COVID-19 vaccine was US$ 60.13 (CI: 56.80–63.46; p < 0.01). Having a higher perceived risk of being contaminated with COVID-19, higher average monthly income, higher education level, pre-existence of chronic diseases, previous experience of vaccination, and belonging to higher age groups were significant determinants associated with WTP for COVID-19 vaccination.

Conclusion

The present study indicates a relatively high WTP and acceptance of a COVID-19 vaccine among the Iranian population. Average monthly income, risk perception, education level, the preexistence of chronic disease, and previous vaccination experience increased the likelihood of WTP for a vaccine. Subsidizing the COVID-19 vaccine for the low-income population and raising risk perception among the population should be considered in formulating vaccine-related interventions.
Advancing socioecological mental health promotion intervention: A mixed methods exploration of Phase 1 Agenda Gap findings
Authors: Jenkins Emily , Daly Zachary , McAuliffe Corey , McGuinness Liza , Richardson Chris , Hill Taylor G. , Goodyear Trevor , Lind Candace , Barbic Skye , Rivers Robert , Haines-Saah Rebecca
Published: February 2023

Introduction

Protecting and promoting the mental health of youth under 30 years of age is a priority, globally. Yet investment in mental health promotion, which seeks to strengthen the determinants of positive mental health and wellbeing, remains limited relative to prevention, treatment, and recovery. The aim of this paper is to contribute empirical evidence to guide innovation in youth mental health promotion, detailing the early outcomes of Agenda Gap, an intervention centering youth-led policy advocacy to influence positive mental health for individuals, families, communities and society.

Methods

Leveraging a convergent mixed methods design, this study draws on data from n = 18 youth (ages 15 to 17) in British Columbia, Canada, who contributed to pre- and post-intervention surveys and post-intervention qualitative interviews following their participation in Agenda Gap from 2020-2021. These data are supplemented by qualitative interviews with n = 4 policy and other adult allies. Quantitative and qualitative data were analyzed in parallel, using descriptive statistics and reflexive thematic analysis, and then merged for interpretation.

Results

Quantitative findings suggest Agenda Gap contributes to improvements in mental health promotion literacy as well as several core positive mental health constructs, such as peer and adult attachment and critical consciousness. However, these findings also point to the need for further scale development, as many of the available measures lack sensitivity to change and are unable to distinguish between higher and lower levels of the underlying construct. Qualitative findings provided nuanced insights into the shifts that resulted from Agenda Gap at the individual, family, and community level, including reconceptualization of mental health, expanded social awareness and agency, and increased capacity for influencing systems change to promote positive mental health and wellbeing.

Discussion

Together, these findings illustrate the promise and utility of mental health promotion for generating positive mental health impacts across socioecological domains. Using Agenda Gap as an exemplar, this study underscores that mental health promotion programming can contribute to gains in positive mental health for individual intervention participants whilst also enhancing collective capacity to advance mental health and equity, particularly through policy advocacy and responsive action on the social and structural determinants of mental health.
Case report: Death caused by 1, 3-dichloropropene, a novel fumigant used in China
Authors: Zhou Zhiqiang , Guo Lanlan , Wen Zixin , Dai Ping , Zhang Tongyue , Wang Wenjun , Jian Xiangdong
Published: February 2023

As an efficient and broad-spectrum soil fumigant, 1, 3-dichloropropene is widely used in the control of nematodes, soil pests, and plant pathogens. However, as a volatile chlorine-containing organic compound, 1, 3-dichloropropene is harmful to human health, although no deaths caused by inhalation of 1, 3-dichloropropene have been reported. This article describes the case of a 50-year-old man who died of acute renal failure and brain edema after inhaling 1, 3-dichloropropene at work. This case demonstrates that 1, 3-dichloropropene can be absorbed through the respiratory tract and that exposure to 1, 3-dichloropropene in a confined environment without any protective measures can cause death in humans.
Workplace infection control measures and romantic activities of workers during COVID-19 pandemic: A prospective cohort study in Japan
Authors: Fujino Yoshihisa , Okawara Makoto , Hino Ayako , Muramatsu Keiji , Nagata Tomohisa , Ikegami Kazunori , Tateishi Seiichiro , Tsuji Mayumi , Ishimaru Tomohiro
Published: February 2023

Objective

Due to the COVID-19 pandemic, non-married people are at high risk of loneliness. With social interactions restricted, it is important for non-married people to acquire a new romantic partner for their mental health and quality of life. We hypothesized that infection control efforts in the workplace influence people's social interactions, including romantic activities.

Methods

We conducted an internet-based prospective cohort study from December 2020 (baseline) to December 2021, using self-administered questionnaires. Briefly, 27,036 workers completed the questionnaires at baseline, and when followed up after 1 year, 18,560 (68.7%) participated. A total of 6,486 non-married individuals with no romantic relationship at baseline were included in the analysis. At baseline they were asked about the implementation of infection control measures in the workplace, and at follow-up they were asked about activities they performed with a view to romantic relationships during the period from baseline to follow-up.

Results

Compared to workers in workplaces with no infection control measures, the odds ratio (OR) associated with romance-related activities for those in workplaces with seven or more infection control measures was 1.90 (95% CI: 1.45–2.48, p < 0.001), and the OR associated with having a new romantic partner was 1.79 (95% CI: 1.20–2.66, p = 0.004).

Discussion

Under the COVID-19 pandemic, the implementation of infection control measures in the workplace and the expressed satisfaction with those measures promoted romantic relationships among non-married, single individuals.
Causal analysis of nitrogen oxides emissions process in coal-fired power plant with LiNGAM
Authors: Saito Tatsuki , Fujiwara Koichi
Published: February 2023

Coal has been an important energy source worldwide; however, it is the largest source of nitrogen oxide (NOx) emissions because the amount of nitrogen in coal is larger than that of other fossil fuels. Precise control of NOx emissions is required in operations of coal-fired power plants from the viewpoint of air pollution control. Although theoretical analyses of NOx generation from a coal-fired power plant have been conducted, it is difficult to precisely predict NOx generation in an actual plant. NOx generation is affected by various factors, such as furnace design and operating conditions, and there are complicated relationships among them. Thus, it is necessary to identify important operating factors that affect NOx generation in actual coal-fired power plants. A linear non-Gaussian acyclic model (LiNGAM) is an exploratory causal analysis method that identifies a causal ordering of variables and their connection strengths without any prior knowledge of causal relationships among variables. In this study, we analyzed real operation data collected from a coal-fired power plant using LiNGAM to identify factors of NOx generation. The causal relationship between process variables and NOx generation was estimated by means of LiNGAM, and the connectional strengths of the variables on NOx generation were derived. The analysis results agreed with previous reports on NOx generation mechanisms, such as combustion air temperature, steam temperature on a specific side of the furnace, and air flow rate of forced draft fans. In addition, we found the steam flow rate and the furnace pressure as new candidate factors of NOx generation through causal analysis using LiNGAM, which heretofore has not been suggested. Our analysis result should contribute to reducing NOx emissions from coal-fired power plants in the future.
Finding alternatives for the traditional diesel-powered company car: A conjoint analysis approach
Authors: De Wilde Liesbeth , Keserü Imre , Macharis Cathy , Vanhaverbeke Lieselot
Published: February 2023

Although the use of company cars is associated with more congestion, pollution and accidents compared to privately owned cars, the Belgian fiscal system provides exceptionally high incentives to company cars. As a result, the proportion of company cars is higher in Belgium than in any other OECD country. With a corporate mobility budget, more sustainable options are being offered as an alternative to large diesel-powered company cars, but little is known about how company car drivers value these alternatives. In this article, we explore how car-dependent employees make their choices in the company mobility system and aim to find options that enable more sustainable commuting. A choice-based conjoint analysis carried out among 422 car dependent company car drivers was used to measure their preference for alternative car-based solutions. The results indicate an overall preference for hybrid cars, but a shift toward fully electric vehicles is necessary to have a significant impact on climate change. Our results suggest that respondents with a higher environmental concern are more eager to make the transition toward smaller and fully electric vehicles, which is in line with previous studies. The study revealed that there is currently no alternative that is both more sustainable and more preferred by the sample, which again stresses the need for more drastic government intervention.
Ecological validity of virtual reality simulations in workstation health and safety assessment
Authors: Personeni Gabin , Savescu Adriana
Published: February 2023

The last decade saw a rapid rise of interest in Virtual Reality (VR) technologies, driven by more mature hardware and software tools. Within the ongoing digitalization of industry, VR technologies see uses in workstation design, operator training and tele-operation. This article focuses on how VR can contribute to workstation design including health and safety assessment. VR allows the inclusion of the operator in the workstation design process, permitting evaluation of the design in a safe, interactive and immersive virtual environment. This systematic literature review aims to qualify the ecological validity of VR tools and identify the current obstacles to safe and successful workstation design transfer. A standard systematic literature review procedure is used, on a wide selection of experimental research articles studying the validity of VR, within or outside of industrial contexts. We aggregate results from fundamental research on VR ecological validity regarding user perceptions, movement, cognition and stress. These results are discussed with respect to their influence on workstation OSH assessment in VR. Furthermore, we identify current technological factors and upcoming developments that mediate the validity of VR assessments.
Bone Biology: From a to Zebrafish
Authors: DuMez Rachel , Skromne Isaac
Published: February 2023

Even though humans look different from other animals like fish, birds, or snakes, we all have a skeleton that gives our bodies shape, protects our internal organs from harm, and helps us move. Animals with a skeleton and a backbone are called vertebrates. Because all skeletons are built from bones, scientists can learn a lot about human bones by studying them in other vertebrates, including fish. Zebrafish are one vertebrate used by scientists to study how bones are made, age, and are repaired by the body. One cool fact about zebrafish is that they can regrow their tails, including the bones, if they get bitten off by another fish. Scientists can also use zebrafish to learn how new medicines can maintain bone health, or how to fix bones after an accident or disease. In this article, we will discuss how scientists can work with zebrafish to learn about bone biology and health.
Can agricultural trade improve total factor productivity? Empirical evidence from G20 countries
Authors: Xu Jialong , Wang Yue , Zhao Xing , Etuah Seth , Liu Zhen , Zhu Hui
Published: February 2023

Improving agricultural total factor productivity is essential to achieving the high-quality and sustainable development of agriculture. As major global agricultural producers, the G20 countries play an important role in agricultural product trade and development. As such, it is well-positioned to play a positive role in improving agricultural total factor productivity. This paper uses the DEA-Malmquist index method to measure agricultural total factor productivity (TFP) in G20 countries from 2010 to 2019, and analyzes the impact of agricultural trade on TFP using the two-way fixed effects model. It finds that (1) the main source of agricultural TFP growth in G20 countries is technical progress, while the effect of technical efficiency on agricultural TFP is not obvious. (2) Agricultural trade can significantly improve agricultural TFP growth in G20 countries, and the effect is more obvious in developed countries. From the perspective of trade flow, the positive effect of export trade on agricultural TFP is stronger. (3) The institutional environment strengthens the improvement effect of agricultural trade on agricultural TFP. Thus, this study not only provides valuable insight into the relationship between agricultural trade and agricultural productivity, but also offers a strong argument in favor of the formulation of relevant policies to improve agricultural productivity and promote a more sustainable agricultural sector.
Microbial consortia enhance the yield of maize under sub-humid rainfed production system of India
Authors: Manjunath Mallappa , Khokhar Anil , Chary Gajjala Ravindra , Singh Manmohanjit , Yadav Sushil Kumar , Gopinath Kodigal A. , Jyothilakshmi Narayana , Srinivas Karlapudi , Prabhakar Mathyam , Singh Vinod Kumar
Published: February 2023

Plant beneficial microorganisms are being used to improve soil health and crop yield in different cropping systems. Maize is an important crop grown around the world for food, feed and raw material for various industries. The aim of the present study was to evaluate two microbial consortia viz., microbial consortia 1 (Pseudomonas putida P7 + Paenibacillus favisporus B30) and microbial consortia 2 (Pseudomonas putida P45 + Bacillus amyloliquefaciens B17) under field conditions for their suitability in improving maize yield under rainfed situations at Ballowal Saunkhri (Punjab) having sub-humid (Hot Dry) climatic conditions. Pooled analysis of three years field experiments data showed that, seed + soil application of microbial consortia 1 and 2 led to enhancement in grain yield of kharif maize by 27.78 and 23.21% respectively over uninoculated control. Likewise, significant increase in Benefit:Cost ratio as well as straw yield was also observed. The present investigation suggests that, microbial consortia would help in significantly improving the yield and economics of maize grown on inceptisols under rainfed conditions.
Integration of lipidomics and metabolomics approaches for the discrimination of harvest time of green tea in spring season by using UPLC-Triple-TOF/MS coupled with chemometrics
Authors: Cui Hui-Na , Gu Hui-Wen , Li Zhi-Quan , Sun Weiqing , Ding Baomiao , Li Zhenshun , Chen Ying , Long Wanjun , Yin Xiao-Li , Fu Haiyan
Published: February 2023

The production season is one of the judgment standards of the green tea quality and spring tea is generally considered of higher quality. Moreover, early spring tea is usually more precious and sells for a higher price. Therefore, a multifaceted strategy that integrates lipidomics and metabolomics, based on UPLC-Triple-TOF/MS coupled with chemometrics, was developed to discriminate early spring green tea (ET) and late spring green tea (LT). Twenty-six lipids and forty-five metabolites were identified as characteristic components. As for characteristic lipids, most of glycerophospholipids and acylglycerolipids have higher contents in ET. By contrast, glycoglycerolipids, sphingolipids and hydroxypheophytin a were shown higher levels in LT samples. Most of the differential metabolites identified were more abundant in ET samples. LT samples have much higher catechin, procyanidin B2, and 3',8-dimethoxyapigenin 7-glucoside contents. Based on the integration of differential lipids and metabolites, the reconstructed orthogonal partial least squares discriminant analysis (OPLS-DA) model displayed 100% correct classification rates for harvest time discrimination of green tea samples. These results demonstrated that the integration of lipidomics and metabolomics approaches is a promising method for the discrimination of tea quality.
Lime, inoculum, and phosphorous input supplementation under rain-fed soybean in Ghana's northern savannas
Authors: Awuni George A. , Reynolds Daniel B. , Dodds Darrin , Denwar Nicholas N. , Abdulai Alhassan L. , Asungre Peter A. , Yahaya Iddrisu , Goldsmith Peter D.
Published: February 2023

Introduction

Recent prices in soybean have spurred interest in motivating growers into input integration to improve yields across the northern savannas of Ghana. This requires more knowledge of input integration to increase soybean yield and profitability across production areas.

Materials and method

A 3-yr study was conducted in three locations at Manga, Nyankpala, and Wa in Ghana's northern savanna to identify the best-yielding input combination under a rain-fed environment. The experiment used “Jenguma” soybean cultivar with lime, inoculum, and phosphorus in a 2 × 2 × 2 factorial arrangement with four replications. Eight treatment combinations (control included) were used to assess soybean yield, yield components, rain use efficiency, and economic profitability. The single-input application of lime (T1), inoculum (T2), and phosphorus (T4) was the low-input, lime × inoculum (T3), lime × phosphorus (T5), and (inoculum × phosphorus) (T6) as medium input, T7 (lime × inoculum × phosphorus) was high input, and a no-input treatment (control).

Results

The soils were inherently low in fertility, and weather variability during the reproductive stages was a significant limiting factor to improving grain yield across locations. Grain yield was highest in 2017 at Manga and Nyankpala, but in 2019 at Wa. Except at Manga, grain yield and rain use efficiency (RUE) were highest in the high input system (T7). However, marginal net benefits (MNB) and marginal rate of returns (MRR) were highest in the low input system (T2) across locations. The low (T1) and medium (T3) input systems (except Nyankpala) were dominant for MNB and MRR due to the high cost of the lime product.

Discussion

These results suggest that smallholder farmers prefer low input (T2) with the least cost of production and the highest marginal net benefit and marginal rate of returns. Nevertheless, producers would have to evaluate their resource base and the cost of nutrient integration for sustainability.

Conclusion

This paper recommends further studies on lime rates to determine profitability, lime's long-term (residual) effect, and the synergic impact of inoculum and phosphorus.
Early surgical outcomes and influencing factors of high tibial osteotomy
Authors: Yan Zhaolong , Gu Yange , He Jiahuan , Zhang Chenyang , Wang Jianye , Zhang Zhenbin , Zhao Zhang , Li Shufeng
Published: February 2023

Objective

To investigate the influencing factors of functional recovery after high tibial osteotomy (HTO).

Methods

A retrospective research was carried on 98 patients who underwent HTO between January 2018 and December 2020. In each case, the medial proximal tibial angle (MPTA), joint line convergence angle (JLCA), femoral tibial angle (FTA), hip-knee-ankle (HKA), weight bearing line (WBL) ratio of the knee joint, opening gap, opening angle, American knee society knee score (KSS), US Hospital for Special Surgery (HSS) score, Lysholm score, and Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) were measured to determine postoperative function and influential factors of pain through logistic regression analysis.

Results

The follow-up time was between 18 and 42 months after operation with an average of 27.66 ± 12.9 per month. Overall functional scores were significantly improved. The influencing factors that may affect the postoperative effect of HTO include age and preoperative WBL ratio of the knee joint (WBL%). After incorporating these two factors into the multivariate logistic regression analysis, for every 1 unit increase in the preoperative WBL%, the probability of postoperative HSS being superior is 1.06 times higher than before [Exp(β): 1.062, 95% CI: 1.01–1.1, p = 0.018]. For every year increase in age, the probability of an excellent HSS score after surgery was 0.84 times higher than that before surgery [Exp(β): 0.843, 95% CI: 0.718–0.989, p = 0.036]. Preoperative WBL% ≥ 14.37 was 17.4 times more likely to be rated as excellent postoperative HSS than that <14.37 [Exp(β): 17.406, 95% CI: 1.621–186.927, p = 0.018].

Conclusion

The postoperative functional scores of the patients significantly improved. Patients with preoperative WBL% ≥ 14.37% had better function after surgery.
Dedicated esophageal imaging may be unnecessary in marijuana-associated spontaneous pneumomediastinum: Findings from a retrospective cohort study
Authors: Yu Irene , Tung Kaity , Dugan Ryanne , Qaqish Robert Thamer , Perry Yaron
Published: February 2023

Background

Marijuana use has become more common since its legalization, as have reports of marijuana-associated spontaneous pneumomediastinum. Non-spontaneous causes such as esophageal perforation are often ruled out on presentation due to the severe consequences of untreated disease. Here we seek to characterize the presentation of marijuana-associated spontaneous pneumomediastinum and explore whether esophageal imaging is necessary in the setting of an often benign course and rising healthcare costs.

Materials and Methods

Retrospective review was performed for all 18–55 year old patients evaluated at a tertiary care hospital between 1/1/2008 and 12/31/2018 for pneumomediastinum. Iatrogenic and traumatic causes were excluded. Patients were divided into marijuana and control groups.

Results

30 patients met criteria, with 13 patients in the marijuana group. The most common presenting symptoms were chest pain/discomfort and shortness of breath. Other symptoms included neck/throat pain, wheezing, and back pain. Emesis was more common in the control group but cough was equally prevalent. Leukocytosis was present in most patients. Four out of eight of computed tomography esophagarams in the control group showed a leak requiring intervention, while only one out of five in the marijuana group showed even a possible subtle extravasation of contrast but this patient ultimately was managed conservatively given the clinical picture. All standard esophagrams were negative. All marijuana patients were managed without intervention.

Discussion

Marijuana-associated spontaneous pneumomediastinum appears to have a more benign clinical course compared to non-spontaneous pneumomediastinum. Esophageal imaging did not change management for any marijuana cases. Perhaps such imaging could be deferred if clinical presentation of pneumomediastinum in the setting of marijuana use is not suggestive of esophageal perforation. Further research into this area is certainly worth pursuing.
Bibliometric insights in fournier's gangrene: Research landscapes, turning points, and global trends
Authors: Zhang Jia-Yuan , Xiao Chang-Fang , Wang Chen , Yao Yi-Bo
Published: February 2023

Study Design

Bibliometric and visualization analysis.

Objective

To analyze the research landscapes and hotspots of Fournier's gangrene, and reveal the dynamic changes and development trend of research hotspots for the purpose of providing ideas and a basis for clinical and basic research in this field.

Methods

Research datasets were acquired from the Web of Science. The publication years were limited from January 1, 1900 to August 5, 2022. The bibliometric tools CiteSpace (v5.8) and VOSviewer (v1.6) were used to analyze the data and generate visualization knowledge maps. Trends in annual publications, distribution, H-index status, coauthorships status and research hotspots were analyzed.

Results

According to the search strategy, we identified and enrolled 688 publications regarding to Fournier's gangrene. The number of published papers showed an overall upwards trend. The USA was the largest contributor, ranking first in total publications, citations and the H-index. The top 10 most productive institutions were all from the USA. De Simone B and Sartelli M were the most productive authors. There was close cooperation among countries, but the cooperation among institutions and authors had little contact and poor interactivity. The research hotspots included the pathogenesis factors and treatment. All the identified keywords were divided into 14 clusters, and the label of the latest cluster was “empagliflozin”. Prognosis and risk factors, emerging treatment methods, and pathogenesis were at the forefront of the Fournier's gangrene field and were predicted to be the next hot topics.

Conclusion

The research of Fournier's gangrene has made some achievements, but the overall research level is still in the primary stage. The academic cooperation between different institutions and authors needs to be strengthened. At the early stage, the mainstream of research was the infected tissue and site, pathogenesis, and diagnosis of disease, while research on newly discovered sodium-glucose cotransporter 2 inhibitor, adjuvant therapy and prognostic factors may be the main directions in the future.
Integrating filter paper extraction, isothermal amplification, and lateral flow dipstick methods to detect Streptococcus agalactiae in milk within 15 min
Authors: Zhu Lingling , Gong Fengju , Liu Xia , Sun Xueqiang , Yu Yong , Shu Jie , Pan Zihao
Published: February 2023

Introduction

Mastitis is one of the most serious diseases affecting dairy farming, causing huge economic losses worldwide. Streptococcus agalactiae is the main pathogenic bacterium of contagious mastitis and can deliver a devastating blow to a farm's economy. Rapid detection is the key to disease control.

Methods

In this study, a rapid detection method for S. agalactiae was established. This method combines filter paper extraction, multienzyme isothermal rapid amplification (MIRA), and lateral flow dipsticks (LFD). To simplify the extraction procedure, we designed a disposable extraction device (DED). First, DED performance was evaluated by polymerase chain reaction (PCR) and then the lysis formula and extraction time were optimized. Second, this study compared the extraction performance of a filter paper and an automatic nucleic acid extraction instrument. After screening primers, MIRA for S. agalactiae was established and combined with LFD. Specificity and sensitivity were evaluated after optimizing the reaction conditions.

Results

The results showed that the lowest extraction line for DED was 0.01–0.001 ng/μl. In the specificity study, 12 different bacteria were tested, and only S. agalactiae was found to be positive. In the sensitivity study, seven dilution gradients were established, and the lowest detection line was 3.52 × 102 CFU/ml.

Discussion

In summary, the method established in this study does not require laboratory equipment and is suitable for on-site detection. The entire method takes only 15 min, is low in cost, has high precision and low technical requirements for operators, which is in contrast with the high cost and cumbersome operation of traditional methods, and is suitable for on-site testing in areas with limited facilities.
Anatomy of hypothalamic and diencephalic nuclei involved in seasonal fertility regulation in ewes
Authors: Merchán Miguel , Coveñas Rafael , Plaza Ignacio , Abecia José Alfonso , Palacios Carlos
Published: February 2023

In this study, we describe in detail the anatomy of nuclei involved in seasonal fertility regulation (SFR) in ewes. For this purpose, the intergeniculate leaflet of the visual thalamus, the caudal hypothalamic arcuate nucleus, and suprachiasmatic, paraventricular and supraoptic nuclei of the rostral hypothalamus were morphometrically and qualitatively analyzed in Nissl-stained serial sections, in the three anatomical planes. In addition, data were collected on calcium-binding proteins and cell phenotypes after immunostaining alternate serial sections for calretinin, parvalbumin and calbindin. For a complete neuroanatomical study, glial architecture was assessed by immunostaining and analyzing alternate sections for glial fibrillary acidic protein (GFAP) and ionized calcium-binding adapter molecule 1 (IBA1). The results showed a strong microglial and astroglia reaction around the hypothalamic nuclei of interest and around the whole 3rd ventricle of the ewe brain. Moreover, we correlated cytoarchitectonic coordinates of panoramic serial sections with their macroscopic localization and extension in midline sagittal-sectioned whole brain to provide guidelines for microdissecting nuclei involved in SFR.
Microfluidic-LAMP chip for the point-of-care detection of gene-deleted and wild-type African swine fever viruses and other four swine pathogens
Authors: Ji Chihai , Zhou Ling , Chen Yonghui , Fang Xueen , Liu Yanhong , Du Mengkan , Lu Xiandong , Li Qianniu , Wang Heng , Sun Yuan , Lan Tian , Ma Jingyun
Published: February 2023

Introduction

Different pathogens causing mixed infection are now threatening the pig industry in the context of the African Swine Fever (ASF) circulating especially in China, and it is crucial to achieving the early diagnosis of these pathogens for disease control and prevention.

Methods

Here we report the development of a rapid, portable, sensitive, high-throughput, and accurate microfluidic-LAMP chip detection system for simultaneous detection and differentiation of gene-deleted type and wild-type African swine fever virus (ASFV), pseudorabie virus (PRV), porcine parvovirus (PPV), porcine circovirus type 2 (PCV2), and porcine reproductive and respiratory syndrome (PRRSV).

Results and discussion

The newly developed system was shown to be sensitive with detection limits of 101 copies/μl for ASFV-MGF505-2R/P72, PPV, and PCV2, 102 copies/μl for ASFV-CD2v, PRV, and PRRSV. The system was highly specific (100%) and stable (C.V.s < 5%) in its ability to detect different pathogens. A total 213 clinical samples and 15 ASFV nucleic acid samples were collected to assess the performance of the detection system, showing highly effective diagnosis. Altogether, the developed microfluidic-LAMP chip system provides a rapid, sensitive, high-throughput and portable diagnostic tool for the accurate detection of multiple swine pathogens.
Three-stage fermentation of the feed and the application on weaned piglets
Authors: Jiang Dahai , Yang Manqi , Xu Jun , Deng Liping , Hu Cong , Zhang Liangliang , Sun Yunzhang , Jiang Jianchun , Lu Liming
Published: February 2023

Numerous studies have demonstrated that soybean meal (SBM) contains high levels of anti-nutritional factors, which interrupt gastrointestinal homeostasis or metabolism normally of the weaned piglets. Here, the mixed probiotics, including Bacillus licheniformis (B. licheniformis, CGMCC 8147), Saccharomyces cerevisiae H11 (S. cerevisiae H11) and Lactobacillus casei (L. casei, CGMCC 8149) were applied to the three-stage fermentation of functional feed. Our research investigated the optimum ratio of inoculation, optimal time of inoculation, combination of substrates, and nutritional value of the fermented feed. The optimal microbial combination was B. licheniformis: S. cerevisiae: L. casei = 2:2:1, inoculating at 0, 12 and 24 h, respectively. The results revealed that crude protein and acid-soluble protein were remarkably improved and had lower pH. Trypsin inhibitor, glycinin and β-conglycinin were reduced by 79.86, 77.18, and 69.29%, respectively. Moreover, animal trials further evaluated the growth-promoting effects of the fermented feed. It was noted that the average daily gain of weaned piglets was significantly higher, and the ratio of feed with weight, diarrhea incidence and mortality were lower significantly. The concentrations of serum immunoglobulin G(IgG), IgA, IgM, Complement C3 and interferon-γ (IFN-γ), and lysozyme activity were all increased. The relative abundance of fecal microbiota improved, especially lactobacillus, which increased the abundance of fecal dominant probiotics. Overall, the fermented feed may be conducive to the growth and health of weaned piglets by improving nutritional value, immunity properties, relative abundance of fecal microflora, and decreasing anti-nutritional factors of feed, thereby making them viable and usable feedstuffs for potential use in livestock industries.