bluuebunny/crossref_metadata_2025 huggingface dataset:
Autism and FND
Authors: Cole Richard
Published: November 2023

Richard is a Clinical Research Fellow whose PhD is looking at symptom trajectories after Traumatic Brain Injury and the relevance of autistic traits, interoception and alexithymia. He has recently finished Core Psychiatry training in Camden & Islington and has returned to part time clinical work in Neurorehabilitation.

Little is known about the prevalence of autism or autistic traits in patients in FND, and studies investigating this have used mixed methods, finding mixed results. My talk will cover why we should be thinking about autism within he FND population, where might lie predisposing or perpetuating factors, what we know so far and how this is relevant for clinical practice.
Psychological inoculation against misinformation
Authors: van der Linden Sander
Published: November 2023

Sander van der Linden is Professor of Social Psychology in Society in the Department of Psychology at the University of Cambridge and Director of the Cambridge Social Decision-Making Lab. Before coming to Cambridge, he held posts at Princeton and Yale University. His research interests center around the psychology of human judgment and decision-making. In particular, he is interested in the social influence and persuasion process and how people are influenced by (mis)information and gain resistance to persuasion through psychological inoculation. He is also interested in the study of fake news, media effects, social networks, and belief systems (e.g., conspiracy theories), as well as the emergence of social norms, polarization, reasoning about evidence, and public understanding of risk and uncertainty. His research spans from social psychology to cognitive science using a variety of techniques, from virtual reality to survey and lab studies to computational social science and large-scale (online) interventions.

His forthcoming book FOOLPROOF: Why Misinformation Infects Our Brains, and How to Build Immunity (WW Norton/HarperCollins) is to be published later this month. He has published around 150 papers and is ranked among the top 1% of all social scientists worldwide (Clarivate ISI Highly Cited Researcher) and among the top 2% across all scientific fields (Ioannidis, Boyack, & Baas, 2020).

Much like a viral contagion, misinformation can spread rapidly from one mind to another. Moreover, once lodged in memory, misinformation is difficult to correct. Inoculation theory therefore offers a natural basis for developing a psychological ‘vaccine’ against the spread of fake news and misinformation. Specifically, in a series of randomized lab and field studies, I’ll show that it is possible to pre-emptively ‘immunize’ people against disinformation about a wide range of topics by pre-exposing them to severely weakened doses of the techniques that underlie its production. This process of ‘prebunking’ helps people cultivate cognitive antibodies in a simulated social media environment. During the talk, I’ll showcase several real-world interventions we developed and empirically evaluated in 20 languages—with governments and social media companies—to help citizens around the world recognize and resist unwanted attempts to influence and mislead.
Understanding the different dimensions of healthcare systems: the ‘interpreting voice’ found in interpretation and translation services
Authors: Rana Lachhemi
Published: November 2023

Interpreting was not something I had aspired to do; however, when I realised that there was a genuine scarcity for people in need of such services, I made a conscious decision to work as a freelance interpreter. The translation and interpreting service operates through various methods, such as face-to-face, video interpreting or remote interpreting by phone, depending on the need and the availability of both the user and the service provider. Usually, the doctors and other healthcare staff who work in the National Health Service book the interpreters through various interpreting companies, if patients are unable to speak the English language to express themselves. Despite immense effort, many times, staff cannot find interpreters for the patients; hence, I feel that awareness should be made so that people can come forward to use their language skills to help those people who require it. Having a medical degree helped me and instilled in me a better understanding, which is why I consciously give my free time to this service. In this article, I have tried to share my experience, so that people can derive a better understanding regarding the interpreting service in the UK. As an interpreter and translator of three languages, I have been working on a freelance basis for the past 3 years in the UK and am a linguist, a registered member of Chartered Institute of Language (CIOL).
Core20PLUS5: His Majesty’s Prison and Probation Service – an approach to address inequities in healthcare for people in contact with the criminal justice system
Authors: Owolabi Bola , Lad Sunil , Bhattacharya Chandraa
Published: November 2023

People involved in the criminal justice system are one of the population target cohorts of Core20PLUS5, a national National Health Service (NHS) England approach to support reduction of healthcare inequalities. For the health and justice system to be socially equitable, fair and just, the leadership across the health landscape has a central role to play to ensure this vulnerable group has an equitable opportunity for improved healthy life expectancy, regardless of their multiple disadvantages. On the 75th year of the inception of the NHS, this article is a call to action to bring about sustainable change through data reporting, digital innovation, accelerating preventative programmes and system leadership in order to achieve equitable access, excellent experience and optimal outcomes. It acknowledges the detrimental impact of crime and the importance of improving a range of health and social outcomes for this group.
Impact of roots on the hydrogeological properties of silty soil covers
Authors: Proteau Alex , Guittonny Marie , Bussière Bruno
Published: November 2023

Mine tailings storage poses significant environmental risks such as the formation of acid mine drainage. Engineered covers offer a solution by controlling water ingress. Their performance is based on specific soil hydrogeological properties (SHPs). They must support vegetation which can impact saturated hydraulic conductivity ( ksat) and water retention curve (WRC). This study assesses the impact of 4-year-old willow root colonization on silty soil covers using flexible wall parameters and water retention tests. The obtained SHPs were compared with root traits. The results suggested that the hydrogeological properties of the studied in situ samples were not significantly affected by roots. The variation of measured and predicted ksat values spanned an order of magnitude, regardless of the root colonization intensity, up to a root length density (RLD) of 2.98 cm/cm3. RLD showed a significant and positive linear relationship with measured ksat values ( R2 = 0.54). However, when root colonization was low (RLD < 1 cm/cm3), RLD was negatively correlated with the nvG ( R2 = 0.44) parameter of the van Genuchten WRC model, while the opposite relationship was observed for samples with RLD > 1 cm/cm3 ( R2 = 0.61). Additionally, RLD and the coarse root (diameter > 1 mm) volume to fine root volume (C/F ratio) influenced WRCs. Over time, coarser roots may have a more pronounced impact on SHPs; further research is needed.
On modelling the cutting forces and impact resistance of honed milling tools
Authors: Hopkins Connor , Clarke Tim , Nguyen Nam , Yussefian Nima Z. , Hosseini Ali
Published: November 2023

In milling operations, cutting tools are subjected to cyclic thermal and mechanical loads due to their intermittent engagement with the workpiece. As a result, they commonly fail due to edge chipping and thermal cracking, among which the former is directly related to the impact at the entry or exit, respectively, in downmilling or upmilling where the chip thickness is maximum. Among the many design factors that affect the impact resistance of milling tools, cutting-edge radius is one of the most important; however, it is often omitted in classic force models. In this paper, a force model that accounts for the edge radius was developed to predict the milling forces. Five sets of milling inserts with custom-made edge radii ranging from 25 to 45 µm were produced and tested. Test results were used to validate the force model and capture the effects of edge radius on the impact resistance of the prepared inserts. Results showed that increasing the edge radius initially improved the impact resistance and increased the tool life. However, increasing the edge radius beyond a certain threshold was proven to be detrimental since it made the tool blunt and drastically increased the cutting forces.
Three-dimensional charged black holes in Gauss–Bonnet gravity
Authors: Jusufi Kimet , Jamil Mubasher , Sheykhi Ahmad
Published: November 2023

Using the zero-point length effect, we construct a new class of charged black hole solutions in the framework of three-dimensional Gauss–Bonnet (GB) gravity with Maxwell electrodynamics. The gravitational and electromagnetic potentials and the spacetime curvature are finite and regular everywhere; however, the computation of scalar curvature invariants suggests the presence of a singularity at the origin. We also explore the thermodynamics of the solutions obtained and reveal that the entropy of the black hole decreases due to the stringy effects. The thermodynamics and conserved quantities are computed, and the validity of the first law of thermodynamics on the black hole horizon is verified. Finally, the spinning black hole solution is reported.
THE ROLE OF ENVIRONMENTAL REGULATION, HETEROGENEOUS TECHNOLOGICAL PROGRESS ON MUNICIPAL INDUSTRIAL WASTEWATER DISCHARGES: EVIDENCE FROM YANGTZE RIVER ECONOMIC BELT IN CHINA
Authors: LI YAN , CHU ZHUJIE , YANG XIAODONG , WU HAITAO , HUANG WEI-CHIAO
Published: November 2023

The large amount of industrial wastewater discharges has significantly limited the sustainable development of China’s resources and posed hazards to human health. In order to contain industrial wastewater discharges, the government has formulated a series of environmental control policies, but the effectiveness of these policies remains a controversial issue. With the rapid development of science and technology, how likely can China rely on technological progress to achieve a win-win outcome for both economic growth and environmental protection? This paper uses data from 105 cities in the Yangtze River Economic Belt over the period 2005–2020 to investigate the effects of environmental regulation and heterogeneous technological progress on industrial wastewater discharges. The results show that environmental regulation and technology introduction effectively reduce industrial wastewater discharges, and independent innovation aggravates industrial wastewater pollution. The interaction analysis reveals that under the influence of environmental regulation, independent innovation will significantly alleviate industrial wastewater discharges in the Yangtze River Economic Belt. In addition, this study also reveals spatial and temporal heterogeneity in the effects of environmental regulations and different types of technological advances on industrial wastewater discharges.
THE EFFECT OF SUSTAINABLE DEVELOPMENT PLANNING ON NATURAL CAPITAL: EVIDENCE FROM CHINA
Authors: AI HONGSHAN , TAN XIAOQING
Published: November 2023

Coordinating ecological protection and economic development is a dilemma for resource-based cities to achieve sustainable development. This paper is the first study to estimate the causal impact of sustainable development planning on natural capital. We take the release of the Sustainable Development Plan for Resource-based Cities (SDPRC) as an exogenous shock and find that the SDPRC significantly increases the Normalized Difference Vegetation Index (NDVI) in resource-based counties. Heterogeneous analyses suggest that the impact is larger in counties with better economic performance, larger scale, and located in western and northeastern regions. Moreover, the effects of the SDPRC in different types of counties on natural capital are heterogeneous. This paper underscores the importance of policies aiming for promoting sustainable development, which increases natural capital and coordinates economic development and ecological protection in resource-based counties.
Physical structure and multiple solitary wave solutions for the nonlinear Jaulent–Miodek hierarchy equation
Authors: Iqbal Mujahid , Seadawy Aly R. , Lu Dianchen , Zhang Zhengdi
Published: November 2023

In this paper, under the observation of extended modified rational expansion method based on symbolic computation, the multiple solitary wave solutions for nonlinear two-dimensional Jaulent–Miodek Hierarchy (JMH) equation are constructed. In this investigation, we use the computer software Mathematica for the construction of multiple solitary wave solutions. The interested and important things in this work are the multiple solitary wave solutions which have various kinds of physical structures such as kink soliton, periodic traveling wave, bright soliton, anti-kink soliton, dark soliton, combined bright and dark solitons, topological soliton and peakon soliton. We are sure that the various kinds of soliton solutions are found first time by using one method in the existing literature works. On the basis of this research, we can say that the applied technique is very efficient, reliable, fruitful and powerful. The constructed soliton solutions for nonlinear JMH equation will play an important role in the investigation of different physical phenomena in nonlinear sciences.
Electromagnetic form factors at low energy elastic electron–proton scattering
Authors: Abdalla A. M. , Eltanany A. M. , Saber A.
Published: November 2023

The prediction of quantum electrodynamics for elastic electron–proton scattering needs further modifications to agree with experimental data. Two scattering mechanisms that may occur are the forward and backward scattering angles for four-momentum transfer [Formula: see text] up to 0.8 (GeV/[Formula: see text])2. This is due to two different scattering centers. But the forward scattering is observed only at relatively high values of [Formula: see text][Formula: see text](GeV/[Formula: see text])2. The modifications related to the electric and magnetic form factors characterize the proton’s internal structure. These form factors’ dependence on [Formula: see text] provides good agreement with other analysis methods. The proton’s charge radius, which corresponds to the maximum value of the scattering radius, is [Formula: see text][Formula: see text]fm. The scattered electron is sensitive to proton internal constituents, especially at high values of [Formula: see text] in which both scattering radius and photon wavelength decrease.
Vibroacoustics Analysis of Plates with Bioinspired Surface Modifications
Authors: Pal Aninda , Ghoshal Ritwik
Published: November 2023

This study presents a simplified semi-analytical model to analyze the dynamic behavior of a plate with shark skin biomimetic surface modifications. The base plate is considered to be thin isotropic homogeneous and modeled using a Kirchhoff–Love plate theory, whereas the dermal denticles are modeled as a distributed array of point mass and replicated using a Dirac delta function. A semi-analytical formulation, which takes into account closed-form beam mode shapes, with the Galerkin technique to minimize errors is utilized to study the free and forced vibration response of modified plates. Harmonic analysis is carried out to understand the dynamic behavior of the modified plate both quantitatively as well as qualitatively. A parametric study varying the array spacing and the boundary conditions is carried out to understand the effect of surface modification on power radiation characteristics. It appears that adding dermal denticles reduces the peak velocity amplitude and peak radiated power at all resonance frequencies. A separate study by varying the base plate material reveals that a higher reduction in the dynamic response and the radiated power can be achieved by increasing the mass ratio. Hence, surface modification using such a shark skin layer may prove beneficial to reduce acoustic radiation and dynamic response and to optimize the design.
Impact of Pretreatment and Drying Factors on Chemical and Biochemical Attributes of Moroccan Thompson Seedless Grapes
Authors: Boudboud Abdelhakim , Aziz Mohamed Ben , Hajjaj Hassan , Hajji Lhoussain , de Meulenaer Bruno , Mazouz Hamid
Published: November 2023

Drying is a common technique in the agrifood industry, but insufficient control in the drying process can result in changes to the fruit’s appearance due to physiological damage during processing. The aim of this study was to investigate the impact of pretreatment and drying process parameters on Moroccan raisins’ quality and safety. The experimental levels of pretreatment factors (blanching, browning agents) and drying temperature were defined at the beginning. Subsequently, a 24-factorial design was employed to provide a simple and reliable model capable of relating directly the response factor (drying time, color intensity change (

E

∗

), chromaticity (

C

∗

), and browning rate) to the variables (NaOH concentration, antibrowning agent concentration, temperature, and relative humidity). All four parameters had a statistically considerable effect on studied responses. Blanching for 5 minutes at 1% of NaOH solution, using an appropriate concentration of antibrowning agent (5% Na2S2O5), and drying at 70°C with 30% of relative moisture can lead to better preservation of grapes’ appearance and quality (chromaticity (

C

∗

) and color change (

E

∗

)). Also, in these conditions, a lower browning rate (14.48%), a lower 5-hydroxymethylfurfural content (12.40 mg/100 g DW), and a higher level of polyphenols (

135.79
±
13.17

 mg GAE/100 g DW) and flavonoid content (

57.81
±
3.08

 mg Qeq/100 g DW) have been recorded while meeting international standards for SO2 content and microbial quality.
Investigating Visible Cane Loss and Stump Damage Due to Sugarcane Chopper Harvester Usage in Thailand
Authors: Rungmekarat Sarawut , Thupwong Kittituch , Chotchutima Songyos , Authapun Jetsada , Yoktham Rinjawan , Thongthip Nanthiga , Jaisuwan Thapakorn , Khawprateep Sombat , Chaisan Ruttanaporn , Chaisan Tanapon
Published: November 2023

Sugarcane farmers face a primary concern regarding cane losses when employing mechanized harvesters. The present study aimed to investigate visible cane losses resulting from the use of large- and medium-sized chopper harvesters. Investigation of the cane losses by sugarcane harvesters was focused on the loss from remaining cane stumps, the extractors, and the elevators. The damage index of sugarcane stumps was investigated in the presented study. Large harvester usage led to cane losses of 2.19, 0.53, and 0.27 t/ha attributed to remaining sugarcane stumps, extractor-stage losses, and elevator-stage losses, respectively. The total cane loss from using large harvesters amounted to 2.99 t/ha (4.0% of total yield). Similarly, the use of medium-sized harvesters led to cane losses of 2.21, 0.44, and 0.20 t/ha attributed to remaining sugarcane stumps, extractor-stage losses, and elevator-stage losses, respectively. The total cane loss from using medium-sized harvesters was 2.85 t/ha (3.4% of total yield). Notably, the primary factor contributing to visible cane loss from sugarcane harvesters was the remaining stumps, which account for 71% and 76% of the total cane loss when using large- and medium-sized harvesters, respectively. The damage index for sugarcane stumps due to large- and medium-sized harvester usage was 0.04 and 0.12, respectively. Overall, these findings emphasize that resolving cane loss and stump damage caused by sugarcane harvesters is crucial for improving harvesting efficiency.
A Concise Review of Nanoparticles Utilized Energy Storage and Conservation
Authors: Mahmud Md. Zobair Al
Published: November 2023

Nanoparticles have revolutionized the landscape of energy storage and conservation technologies, exhibiting remarkable potential in enhancing the performance and efficiency of various energy systems. This review explores the versatile applications of nanoparticles in three key domains: battery technologies, supercapacitors, and solar energy conversion. In the realm of battery technologies, nanostructured particles have emerged as crucial catalysts and electrode materials, significantly elevating the energy density, cycling stability, and charge/discharge rates of batteries. By manipulating the surface chemistry and structure of nanoparticles, researchers have achieved breakthroughs in overcoming traditional limitations, paving the way for next-generation high-capacity and long-lasting batteries. The integration of tiny particles in supercapacitors has led to remarkable advancements in energy storage and rapid energy delivery. Nanoparticle-based electrodes have exhibited exceptional surface area, porosity, and conductivity, contributing to enhanced energy and power densities. The synergy of nanomaterials with novel electrolytes has also extended the operational lifespan of supercapacitors, addressing concerns regarding energy loss over cycles. Furthermore, nanoparticles have played a pivotal role in the field of solar energy conversion. In photovoltaics, nanoparticles with tailored optoelectronic properties have enabled improved light absorption, charge separation, and electron transport, ultimately boosting the efficiency of solar cells. Moreover, nanoparticles have been employed as catalysts in photocatalytic systems for solar fuel generation, driving the sustainable production of clean energy carriers. In this concise review, we highlight the recent advancements, challenges, and future prospects of nanoparticles in these critical energy domains. While the transformative impact of nanoparticles is evident, several challenges such as large-scale synthesis, cost-effectiveness, and long-term stability must be systematically addressed to ensure their seamless integration into practical energy applications. As researchers continue to explore novel synthesis techniques and innovative nanoarchitectures, nanoparticles are poised to reshape the energy landscape, accelerating the transition toward a more sustainable and efficient energy future.
Improving Patient Satisfaction and Associated Factors at Outpatient Department in General Hospitals of Central Zone, Tigray, Northern Ethiopia, June 2018‐August 2019: Pre‐ and Postinterventional Study
Authors: Kidanemariam Gebregziabher , Gebrekidan Hailemikael , Dagnazgi Ebud Ayele , Asfaw Kahsay
Published: November 2023

Background. Typically, the idea of patient satisfaction is employed to evaluate quality. When patients enter hospitals, they have certain demands for treatment. However, patients may become dissatisfied if their requirements or expectations are not met. There is increasing agreement that evaluating hospital services should be based in part on patients’ assessments of the quality of treatment they received overall. The aim of this study was to improve patient satisfaction at outpatient department. Objective. To assess improving of patient satisfaction and associated factors at outpatient department in general hospitals of central zone, Northern Ethiopia, 2019. Methods. Pre‐ and post‐intervention study was conducted to assess the patient satisfaction at outpatient department in general hospitals of central zone, between June 2018 and April 2019 using systematic random sampling method. Two hundred seventy‐five (275) participants were investigated in the preintervention and postintervention study. Data were entered to SPSS version 20. Binary logistic regression was done to test association of factors with the outcome variable with consideration of p value of less than 0.05. Result. In the preintervention period, the patient satisfaction was 54.2%; after providing intervention, the patient satisfaction was increased to 77% in postinterventional study. Respondents who paid for the medical service were 41% less likely satisfied than those who had gotten free services. Participants whose age of 18‐27 years were 22% more likely satisfied than whose age were 58 and greater. Conclusion. The result in this study shows that the patient satisfaction is higher than other studies done in our country. Sex, age, and those who make payment were significantly associated with patient satisfaction. Despite the result, much things are left to be covered to increase satisfaction, so the concerned bodies, including the regional health bureau, woreda health office, and management committee and board, should mobilize the community and give training to the health professionals to make the environment smoother and more comfortable for patients.
Uncropped version of all Western blots from Oncogenic KRASG12D Reprograms Lipid Metabolism by Upregulating SLC25A1 to Drive Pancreatic Tumorigenesis
Authors: Zhang Ruowen , Peng Xiaogang , Du James Xianxing , Boohaker Rebecca , Estevao Igor L. , Grajeda Brian I. , Cox Marc B. , Almeida Igor C. , Lu Weiqin
Published: November 2023

Uncropped version of all Western blots
Supplementary Data from Oncogenic KRASG12D Reprograms Lipid Metabolism by Upregulating SLC25A1 to Drive Pancreatic Tumorigenesis
Authors: Zhang Ruowen , Peng Xiaogang , Du James Xianxing , Boohaker Rebecca , Estevao Igor L. , Grajeda Brian I. , Cox Marc B. , Almeida Igor C. , Lu Weiqin
Published: November 2023

Supplementary Figures
Table S7 from A Cell Surface-Binding Antibody Atlas Nominates a MUC18-Directed Antibody–Drug Conjugate for Targeting Melanoma
Authors: Shi Jing , Jiao Tao , Guo Qian , Weng Weining , Ma Linjie , Zhang Qing , Wang Lijun , Zhang Jianjian , Chen Caiwei , Huang Yaling , Wang Mingqiao , Pan Rong , Tang Yanfang , Hu Wenhao , Meng Tao , Liu Shu-Hui , Guo Jun , Kong Yan , Meng Xun
Published: November 2023

Summary of reported MUC18-targeting functional antibodies
Table S6 from A Cell Surface-Binding Antibody Atlas Nominates a MUC18-Directed Antibody–Drug Conjugate for Targeting Melanoma
Authors: Shi Jing , Jiao Tao , Guo Qian , Weng Weining , Ma Linjie , Zhang Qing , Wang Lijun , Zhang Jianjian , Chen Caiwei , Huang Yaling , Wang Mingqiao , Pan Rong , Tang Yanfang , Hu Wenhao , Meng Tao , Liu Shu-Hui , Guo Jun , Kong Yan , Meng Xun
Published: November 2023

Therapeutic index calculation
Supplementary video 3 from Stroma-Mediated Breast Cancer Cell Proliferation Indirectly Drives Chemoresistance by Accelerating Tumor Recovery between Chemotherapy Cycles
Authors: Miroshnychenko Daria , Miti Tatiana , Kumar Pragya , Miller Anna , Laurie Mark , Giraldo Nathalia , Bui Marilyn M. , Altrock Philipp M. , Basanta David , Marusyk Andriy
Published: November 2023

Time lapse video of MDA468 cells response to a 6 hour exposure to 0.125 μM doxorubicin, in control media. Microscopy photos were taken every 12 hours.
Supplementary video 2 from Stroma-Mediated Breast Cancer Cell Proliferation Indirectly Drives Chemoresistance by Accelerating Tumor Recovery between Chemotherapy Cycles
Authors: Miroshnychenko Daria , Miti Tatiana , Kumar Pragya , Miller Anna , Laurie Mark , Giraldo Nathalia , Bui Marilyn M. , Altrock Philipp M. , Basanta David , Marusyk Andriy
Published: November 2023

Supplementary video 2
Supplementary Data from A Cell Surface-Binding Antibody Atlas Nominates a MUC18-Directed Antibody–Drug Conjugate for Targeting Melanoma
Authors: Shi Jing , Jiao Tao , Guo Qian , Weng Weining , Ma Linjie , Zhang Qing , Wang Lijun , Zhang Jianjian , Chen Caiwei , Huang Yaling , Wang Mingqiao , Pan Rong , Tang Yanfang , Hu Wenhao , Meng Tao , Liu Shu-Hui , Guo Jun , Kong Yan , Meng Xun
Published: November 2023

Supplementary Figures S1 - S13
Supplementary Data from Galectin-3 Cooperates with CD47 to Suppress Phagocytosis and T-cell Immunity in Gastric Cancer Peritoneal Metastases
Authors: Fan Yibo , Song Shumei , Li Yuan , Dhar Shilpa S. , Jin Jiankang , Yoshimura Katsuhiro , Yao Xiaodan , Wang Ruiping , Scott Ailing W. , Pizzi Melissa Pool , Wu Jingjing , Ma Lang , Calin George A. , Hanash Samir , Wang Linghua , Curran Michael , Ajani Jaffer A.
Published: November 2023

Supplementary figure
Data from Targeting the Metabolic Enzyme PGAM2 Overcomes Enzalutamide Resistance in Castration-Resistant Prostate Cancer by Inhibiting BCL2 Signaling
Authors: Li Zhen , Ning Kang , Zhao Diwei , Zhou Zhaohui , Zhao Junliang , Long Xingbo , Yang Zhenyu , Chen Dong , Cai XinYang , Hong Lexuan , Zhang Luyao , Zhou Fangjian , Wang Jun , Li Yonghong
Published: November 2023

Abstract
The next-generation androgen receptor (AR) inhibitor enzalutamide is the mainstay treatment for metastatic prostate cancer. Unfortunately, resistance occurs rapidly in most patients, and once resistance occurs, treatment options are limited. Therefore, there is an urgent need to identify effective targets to overcome enzalutamide resistance. Here, using a genome-wide CRISPR-Cas9 library screen, we found that targeting a glycolytic enzyme, phosphoglycerate mutase PGAM2, significantly enhanced the sensitivity of enzalutamide-resistant prostate cancer cells to enzalutamide both in vivo and in vitro. Inhibition of PGAM2 together with enzalutamide treatment triggered apoptosis by decreasing levels of the antiapoptotic protein BCL-xL and increasing activity of the proapoptotic protein BAD. Mechanistically, PGAM2 bound to 14–3-3ζ and promoted its interaction with phosphorylated BAD, resulting in activation of BCL-xL and subsequent resistance to enzalutamide-induced apoptosis. In addition, high PGAM2 expression, which is transcriptionally regulated by AR, was associated with shorter survival and rapid development of enzalutamide resistance in patients with prostate cancer. Together, these findings provide evidence of a nonmetabolic function of PGAM2 in promoting enzalutamide resistance and identify PGAM2 inhibition as a promising therapeutic strategy for enzalutamide-resistant prostate cancer.
Significance:
PGAM2 promotes resistance to enzalutamide by activating antiapoptotic BCL-xL and suppressing apoptosis, indicating that PGAM2 is a potential target for overcoming enzalutamide resistance in prostate cancer.
The Antipsychotic Drug Penfluridol Inhibits N-Linked Glycoprotein Processing and Enhances T-cell–Mediated Tumor Immunity
Authors: Xu Wenlong , Wang Yuqi , Zhang Na , Lin Xiaofeng , Zhu Di , Shen Cheng , Wang Xiaobo , Li Haiyang , Xue Jinjiang , Yu Qian , Lu Xinyi , Zhou Lu , He Qingli , Tang Zhijun , He Shaodan , Fan Jianjun , Pan Jianbo , Tang Jiangjiang , Jiang Wei , Ye Mingliang , Lu Fanghui , Li Zengxia , Dang Yongjun
Published: November 2023

Aberrant N-linked glycosylation is a prominent feature of cancers. Perturbance of oligosaccharide structure on cell surfaces directly affects key processes in tumor development and progression. In spite of the critical role played by N-linked glycans in tumor biology, the discovery of small molecules that specifically disturbs the N-linked glycans is still under investigation. To identify more saccharide-structure-perturbing compounds, a repurposed drug screen by using a library consisting of 1530 FDA-approved drugs was performed. Interestingly, an antipsychotic drug, penfluridol, was identified as being able to decrease cell surface wheat germ agglutinin staining. In the presence of penfluridol, cell membrane glycoproteins programmed death-ligand 1 (PD-L1) shifted to a lower molecular weight. Further studies demonstrated that penfluridol treatment caused an accumulation of high-mannose oligosaccharides, especially Man5–7GlcNAc2 glycan structures. Mechanistically, this effect is due to direct targeting of MAN1A1 mannosidase, a Golgi enzyme involved in N-glycan maturation. Moreover, we found that altered glycosylation of PD-L1 caused by penfluridol disrupted interactions between programmed cell death protein 1 and PD-L1, resulting in activation of T-cell tumor immunity. In a mouse xenograft and glioma model, penfluridol enhanced the antitumor effect of the anti–PD-L1 antibody in vivo. Overall, these findings revealed an important biological activity of the antipsychotic drug penfluridol as an inhibitor of glycan processing and proposed a repurposed use of penfluridol in antitumor therapy through activation of T-cell immunity.
Separating the Good from the Bad: Tumor-Infiltrating Tregs Have Increased Fucosylation
Authors: Silveria Stephanie , DuPage Michel
Published: November 2023

Regulatory T cells (Treg) can suppress antitumor immune responses, and their presence in tumors is associated with worse prognoses in most cancers. Strategies to neutralize Treg-mediated suppression in tumors without immune-related adverse events, however, are challenging due to the essential role of Tregs in maintaining immune homeostasis. In this issue, Pinioti and colleagues identify fucosylation as a critical regulator of Treg function in tumors that can be targeted therapeutically without impacting immune homeostasis.

See related article by Pinioti et al., p. 1611 (3) .
Supplementary Figure 8 from Ultra-Deep Sequencing Reveals the Mutational Landscape of Classical Hodgkin Lymphoma
Authors: Gomez Felicia , Fisk Bryan , McMichael Joshua F. , Mosior Matthew , Foltz Jennifer A. , Skidmore Zachary L. , Duncavage Eric J. , Miller Christopher A. , Abel Haley , Li Yi-Shan , Russler-Germain David A. , Krysiak Kilannin , Watkins Marcus P. , Ramirez Cody A. , Schmidt Alina , Martins Rodrigues Fernanda , Trani Lee , Khanna Ajay , Wagner Julia A. , Fulton Robert S. , Fronick Catrina C. , O'Laughlin Michelle D. , Schappe Timothy , Cashen Amanda F. , Mehta-Shah Neha , Kahl Brad S. , Walker Jason , Bartlett Nancy L. , Griffith Malachi , Fehniger Todd A. , Griffith Obi L.
Published: November 2023

Comparison of Genes Shown to be Recurrently Mutated in Studies of Adult cHL
Supplementary Figure 5 from Ultra-Deep Sequencing Reveals the Mutational Landscape of Classical Hodgkin Lymphoma
Authors: Gomez Felicia , Fisk Bryan , McMichael Joshua F. , Mosior Matthew , Foltz Jennifer A. , Skidmore Zachary L. , Duncavage Eric J. , Miller Christopher A. , Abel Haley , Li Yi-Shan , Russler-Germain David A. , Krysiak Kilannin , Watkins Marcus P. , Ramirez Cody A. , Schmidt Alina , Martins Rodrigues Fernanda , Trani Lee , Khanna Ajay , Wagner Julia A. , Fulton Robert S. , Fronick Catrina C. , O'Laughlin Michelle D. , Schappe Timothy , Cashen Amanda F. , Mehta-Shah Neha , Kahl Brad S. , Walker Jason , Bartlett Nancy L. , Griffith Malachi , Fehniger Todd A. , Griffith Obi L.
Published: November 2023

Exome Tumor VAF Distribution and Exome Tumor Coverage Partitioned by Validation Status
Supplementary Figure 14 from Ultra-Deep Sequencing Reveals the Mutational Landscape of Classical Hodgkin Lymphoma
Authors: Gomez Felicia , Fisk Bryan , McMichael Joshua F. , Mosior Matthew , Foltz Jennifer A. , Skidmore Zachary L. , Duncavage Eric J. , Miller Christopher A. , Abel Haley , Li Yi-Shan , Russler-Germain David A. , Krysiak Kilannin , Watkins Marcus P. , Ramirez Cody A. , Schmidt Alina , Martins Rodrigues Fernanda , Trani Lee , Khanna Ajay , Wagner Julia A. , Fulton Robert S. , Fronick Catrina C. , O'Laughlin Michelle D. , Schappe Timothy , Cashen Amanda F. , Mehta-Shah Neha , Kahl Brad S. , Walker Jason , Bartlett Nancy L. , Griffith Malachi , Fehniger Todd A. , Griffith Obi L.
Published: November 2023

Observed COSMIC v.3 mutation signatures
Supplementary Figure 12 from Ultra-Deep Sequencing Reveals the Mutational Landscape of Classical Hodgkin Lymphoma
Authors: Gomez Felicia , Fisk Bryan , McMichael Joshua F. , Mosior Matthew , Foltz Jennifer A. , Skidmore Zachary L. , Duncavage Eric J. , Miller Christopher A. , Abel Haley , Li Yi-Shan , Russler-Germain David A. , Krysiak Kilannin , Watkins Marcus P. , Ramirez Cody A. , Schmidt Alina , Martins Rodrigues Fernanda , Trani Lee , Khanna Ajay , Wagner Julia A. , Fulton Robert S. , Fronick Catrina C. , O'Laughlin Michelle D. , Schappe Timothy , Cashen Amanda F. , Mehta-Shah Neha , Kahl Brad S. , Walker Jason , Bartlett Nancy L. , Griffith Malachi , Fehniger Todd A. , Griffith Obi L.
Published: November 2023

Normalized Expression of EIF4A2
Supplementary Figure 11 from Ultra-Deep Sequencing Reveals the Mutational Landscape of Classical Hodgkin Lymphoma
Authors: Gomez Felicia , Fisk Bryan , McMichael Joshua F. , Mosior Matthew , Foltz Jennifer A. , Skidmore Zachary L. , Duncavage Eric J. , Miller Christopher A. , Abel Haley , Lee Yi-Shan , Russler-Germain David A. , Krysiak Kilannin , Watkins Marcus P. , Ramirez Cody A. , Schmidt Alina , Martins Rodrigues Fernanda , Trani Lee , Khanna Ajay , Wagner Julia A. , Fulton Robert S. , Fronick Catrina C. , O'Laughlin Michelle D. , Schappe Timothy , Cashen Amanda F. , Mehta-Shah Neha , Kahl Brad S. , Walker Jason , Bartlett Nancy L. , Griffith Malachi , Fehniger Todd A. , Griffith Obi L.
Published: November 2023

Summary of Recurrently Mutated Genes for Relapsed Samples
Supplementary Figure 10 from Ultra-Deep Sequencing Reveals the Mutational Landscape of Classical Hodgkin Lymphoma
Authors: Gomez Felicia , Fisk Bryan , McMichael Joshua F. , Mosior Matthew , Foltz Jennifer A. , Skidmore Zachary L. , Duncavage Eric J. , Miller Christopher A. , Abel Haley , Li Yi-Shan , Russler-Germain David A. , Krysiak Kilannin , Watkins Marcus P. , Ramirez Cody A. , Schmidt Alina , Martins Rodrigues Fernanda , Trani Lee , Khanna Ajay , Wagner Julia A. , Fulton Robert S. , Fronick Catrina C. , O'Laughlin Michelle D. , Schappe Timothy , Cashen Amanda F. , Mehta-Shah Neha , Kahl Brad S. , Walker Jason , Bartlett Nancy L. , Griffith Malachi , Fehniger Todd A. , Griffith Obi L.
Published: November 2023

Comparison of mutation burden and EBV status
FIGURE 2 from Ultra-Deep Sequencing Reveals the Mutational Landscape of Classical Hodgkin Lymphoma
Authors: Gomez Felicia , Fisk Bryan , McMichael Joshua F. , Mosior Matthew , Foltz Jennifer A. , Skidmore Zachary L. , Duncavage Eric J. , Miller Christopher A. , Abel Haley , Li Yi-Shan , Russler-Germain David A. , Krysiak Kilannin , Watkins Marcus P. , Ramirez Cody A. , Schmidt Alina , Martins Rodrigues Fernanda , Trani Lee , Khanna Ajay , Wagner Julia A. , Fulton Robert S. , Fronick Catrina C. , O'Laughlin Michelle D. , Schappe Timothy , Cashen Amanda F. , Mehta-Shah Neha , Kahl Brad S. , Walker Jason , Bartlett Nancy L. , Griffith Malachi , Fehniger Todd A. , Griffith Obi L.
Published: November 2023

Recurrently mutated genes in Hodgkin lymphoma. The frequency and type of mutations affecting genes mutated in 3 or more patients in our cohort are shown in each row. Genes determined to be significantly mutated using MuSiC and/or dN/dScv (FDR < 0.05) are highlighted (SMG = significantly mutated gene). The open SMG shape indicates that a gene was determined to be an SMG only when HL-513 was excluded from the statistical analysis. The filled SMG shape indicates a gene that was determined to be an SMG only when HL-513 was included in the statistical analysis. The darkest SMG shape indicates that a gene was determined to be an SMG in all analyses (including and excluding HL-513). The bar graph on the right summarizes the frequency of mutations for that gene across the entire cohort. For genes with multiple mutations in a single patient, only one mutation type is shown, prioritized by the order listed in the legend.
A Case of Gastric Perforation Caused by Excessive Insufflation during Upper Gastrointestinal Endoscopy: Nonoperative Management
Authors: Park Youn Su
Published: November 2023

Introduction: Upper gastrointestinal endoscopy is used to diagnose and treat upper gastrointestinal diseases, but it can also cause complications, including perforation. Perforation is a serious complication, so it is important to be aware of the risk factors in advance and to take precautions to prevent it. Case Presentation: We report a case of gastric perforation caused by excessive insufflation during upper gastrointestinal endoscopy in a 64-year-old man with a history of multiple active gastric ulcers. During the endoscopy, the patient did not cooperate and insufflation was performed for a prolonged period of time. This led to a mucosal laceration and a hole suspected to be a perforation on the lesser curvature of the gastric body. The patient was treated nonsurgically with fasting, intravenous fluids, antibiotics, intravenous proton pump inhibitors, and blood transfusions. No leakage was observed on the follow-up computed tomography scan, and the perforation site was completely healed 2 months later. Conclusion: In this case, the patient was successfully treated with conservative therapy alone. The treatment of gastric perforation caused by endoscopy has shifted toward conservative therapy in recent years.
Exploring a Unique RUNX1 Germline Mutation with a Double Nucleotide Variant: Implications for Clinical Significance
Authors: Fujishita Keigo , Inoue Yuusuke , Matsumoto Ken , Oka Satoshi , Machida Takuya , Fuji Shigeo , Yokota Takafumi , Taketani Takeshi , Imai Toshi
Published: November 2023

Introduction: Myeloid neoplasms with germline RUNX1 mutation, an autosomal dominant disease characterized by thrombocytopenia and risk of hematologic malignancy, usually have a single nucleotide variant or insertions/deletions in RUNX1. Here, we present a patient with double nucleotide variant (DNV) in RUNX1. Case Presentation: The patient was a woman with progressive thrombocytopenia. She had a strong family history of thrombocytopenia and hematologic malignancies with a high fatality rate. We detected that genomic RUNX1 had consecutive DNV (c.352G>T and c.352-1 G>C) at its splice site, resulting in a frameshift mutation in exon 5. She rapidly progressed to a high-risk myeloid neoplasm owing to a germline RUNX1 mutation. Allogeneic transplantation successfully resulted in durable complete remission. Conclusion: To our knowledge, this is the first report of hereditary myeloid neoplasm carrying DNV in RUNX1. A family history of thrombocytopenia and the rapid malignant transformation suggests the potential pathogenic significance of the DNV.
Factors Associated with Dialysis Initiation in Patients with Predialysis Arteriovenous Fistula
Authors: Lu Wanhong , Dang Xiangyun , Liu Chao , Wang Zhigang , Lv Jing , Yang Shifeng , Li Huixian
Published: November 2023

Introduction: A large proportion of patients initiated hemodialysis with a central vein catheter rather than a permanent vascular access which was recommended by guidelines. One major barrier was the paucity of evidence regarding the optimal timing of vascular access creation in predialysis patients. Methods: Our study prospectively enrolled 300 patients undergoing predialysis arteriovenous fistula (AVF) creation in our center from 2015 to 2018. Cox proportional hazard regression was performed to identify which demographic and clinical factors were associated with the initiation of hemodialysis after AVF surgery. A receiver operating characteristic area under the curve (AUC) was used to assess the predictive power of preoperative factors for the likelihood of hemodialysis initiation. Results: Overall, 163 (54.3%), 214 (71.3%), and 275 (91.7%) patients initiated hemodialysis within 3 months, 6 months, and 1 year, respectively, after AVF creation. The median time between AVF creation and hemodialysis start was 71.5 days. Using multivariate Cox regression analysis, three factors were associated with hemodialysis initiation within 1 year: serum phosphorus (HR = 1.407, p = 0.021), diabetic kidney disease (DKD) (HR = 1.429, p = 0.039), and cystatin C (HR = 1.179, p = 0.009). Cystatin C alone had a moderate predictive value for dialysis initiation (AUC = 0.746; p < 0.001), whereas the full model had a higher predictive value (AUC = 0.800; p < 0.001). Conclusion: DKD, serum cystatin C, and phosphorus at access surgery were associated with hemodialysis initiation within 1 year of the predialysis AVF creation. Our findings provide a basis for a more customized approach to planning AVF placement in patients with chronic kidney disease.
A Discourse of Beauty Standards in Japanese Beauty Product Advertise-ment (Japanese Women Perspective)
Authors: Paramita Ni Made Savitri , Lestari Eka Marthanty Indah
Published: November 2023

This study uses a Fairclough Critical Discourse Analysis to analyze advertisements' textual and visual elements and discursive and sociocultural analysis of beauty product advertisements in Japan. Data were taken and studied from several examples of beauty product advertisements and interviews with seven Japanese women as informants about the discourse in beauty product advertisements. In addition, the speakers were also asked to express their opinions regarding the beauty standards in Japanese society today.

The results show that advertising has a role in reproducing and maintaining the standard of beauty in Japanese society. The image of women with clean and shining white skin is still the standard for Japanese women. Taglines and beauty products in Japan offer clean white skin as demanded in Japanese society. From this, it is also known that advertising producers use beauty constructions formed in society to make advertisements for beauty products. This causes the discourse on Japanese beauty standards to persist in Japanese society.
Teaching Style and Performance of The Faculty of a Technical School in Ce-bu City
Authors: Narsico Lalaine O. , Narsico Peter G. , Polinar Mark Anthony N.
Published: November 2023

Educators should use different teaching methods to engage students in meaningful learning experiences and promote environmental awareness. This study sought to assess the manifestation of teaching styles and teaching performance in a technical school and identify areas for enhancement. Utilizing a descriptive survey method, teachers, students, and administrators were asked to complete questionnaires to provide data for the study. The results indicated that teaching styles and teaching performance components were highly rated, with teaching styles being frequently observed and teaching performance being excellent. Notably, there was no significant difference in responses from the three groups surveyed. To facilitate further improvement, the technical school is encouraged to identify relevant components for their specific context, establish core principles for each component, organize regular workshops and seminars, periodically evaluate performance to monitor progress and establish mechanisms for continuous development.
Employability of Computer Science Graduates of President Ramon Magsaysay State University, Iba, Zambales from 2017-2021
Authors: Pamplona Carl Angelo S. , Sepillo Geoffrey S. , Dela Cruz Menchie A.
Published: November 2023

This study sought to determine the employment status of Bachelor of Science in Computer Science Graduates at President Ramon Magsaysay State University (PRMSU) Iba Zambales from 2017-2021. Additionally, it also analyzed the relevance of the graduate respondents' existing jobs and investigated the causes of their unemployment. The findings of this study will serve as a platform for future studies aimed at identifying the problems that graduates experience when looking for work, evaluating the performance of educators, and refining the Bachelor of Science in Computer Science curricula to align with the requirements of the industry 4.0 era. The researchers administered a survey to collect data on employment and job placement from the respondents. The results revealed that 46.48 percent of graduate respondents were presently employed in rank-and-file or clerical positions, while 36.62% held positions with professional, technical, and administrative roles directly related to their academic background. Additionally, 57.14 percent of unemployed graduate respondents cited their decision of not finding a job as the reason for being unemployed. The graduates were able to utilize the theoretical and technical proficiencies they learned throughout their academic training. It is highly recommended that further research be undertaken in future studies to enhance the study.