bluuebunny/crossref_metadata_2025 huggingface dataset:
A Minimally Destructive Protocol for DNA Extraction from Ancient Teeth
Authors: Harney Éadaoin , Cheronet Olivia , Fernandes Daniel M. , Sirak Kendra , Mah Matthew , Bernardos Rebecca , Adamski Nicole , Broomandkhoshbacht Nasreen , Callan Kimberly , Lawson Ann Marie , Oppenheimer Jonas , Stewardson Kristin , Zalzala Fatma , Anders Alexandra , Candilio Francesca , Constantinescu Mihai , Coppa Alfredo , Ciobanu Ion , Dani János , Gallina Zsolt , Genchi Francesco , Nagy Emese Gyöngyvér , Hajdu Tamás , Hellebrandt Magdolna , Horváth Antónia , Király Ágnes , Kiss Krisztián , Kolozsi Barbara , Kovács Péter , Köhler Kitti , Lucci Michaela , Pap Ildikó , Popovici Sergiu , Raczky Pál , Simalcsik Angela , Szeniczey Tamás , Vasilyev Sergey , Virag Cristian , Rohland Nadin , Reich David , Pinhasi Ron
Published: August 2020

Ancient DNA sampling methods—although optimized for efficient DNA extraction—are destructive, relying on drilling or cutting and powdering (parts of) bones and teeth. As the field of ancient DNA has grown, so have concerns about the impact of destructive sampling of the skeletal remains from which ancient DNA is obtained. Due to a particularly high concentration of endogenous DNA, the cementum of tooth roots is often targeted for ancient DNA sampling, but standard destructive sampling methods often result in the loss of at least one entire root. Here, we present a minimally destructive method for extracting ancient DNA from dental cementum present on the surface of tooth roots. This method does not require destructive drilling or grinding, and, following extraction, the tooth remains safe to handle and suitable for most morphological studies, as well as other biochemical studies, such as radiocarbon dating. We extracted and sequenced ancient DNA from 30 teeth (and 9 corresponding petrous bones) using this minimally destructive extraction method in addition to a typical tooth sampling method. We find that the minimally destructive method can provide ancient DNA that is of comparable quality to extracts produced from teeth that have undergone destructive sampling processes. Further, we find that a rigorous cleaning of the tooth surface combining diluted bleach and UV light irradiation seems sufficient to minimize external contaminants usually removed through the physical removal of a superficial layer when sampling through regular powdering methods.
Mechanism of negative membrane curvature generation by I-BAR domains
Authors: Nepal Binod , Sepehri Aliasghar , Lazaridis Themis
Published: August 2020

The membrane sculpting ability of BAR domains has been attributed to the intrinsic curvature of their banana-shaped dimeric structure. However, there is often a mismatch between this intrinsic curvature and the diameter of the membrane tubules generated. I-BAR domains have been especially mysterious: they are almost flat but generate high negative membrane curvature. Here, we use atomistic implicit-solvent computer modeling to show that the membrane bending of the IRSP53 I-BAR domain is dictated by its higher oligomeric structure, whose curvature is completely unrelated to the intrinsic curvature of the dimer. Two other I-BARs gave similar results, whereas a flat F-BAR sheet developed a concave membrane binding interface, consistent with its observed positive membrane curvature generation. Laterally interacting helical spirals of I-BAR dimers on tube interiors are stable and have an enhanced binding energy that is sufficient for membrane bending to experimentally observed tubule diameters at a reasonable surface density.
Lactobacillus rhamnosusattenuates bone loss and maintains bone health by skewing Tregs-Th17 cell balance in Ovx mice
Authors: Sapra Leena , Dar Hamid Y. , Pandey Amit , Kumari Surbhi , Azam Zaffar , Bhardwaj Asha , Shukla Prashant , Mishra Pradyumna K. , Verma Bhupendra , Srivastava Rupesh K.
Published: August 2020

Osteoporosis is a systemic-skeletal disorder characterized by enhanced fragility of bones leading to increased rates of fractures and morbidity in large number of populations. Probiotics are known to be involved in management of various-inflammatory diseases including osteoporosis. But no study till date had delineated the immunomodulatory potential ofLactobacillus rhamnosus(LR) in bone-health. In the present study, we examine the effect of probiotic-LR on bone-health in osteoporotic (Ovx) mice model. We observed that administration of LR attenuated bone-loss in Ovx mice. Both the cortical and trabecular bone-content of LR treated group was significantly higher than Ovx-group. Remarkably, the percentage of osteoclastogenic-CD4+Rorγt+Th17 cells at distinct immunological sites such as BM, spleen, LN and PP were significantly reduced, whereas the percentage of anti-osteoclastogenic-CD4+Foxp3+Tregs and CD8+Foxp3+Tregs were significantly enhanced in LR-treated group thereby resulting in inhibition of bone-loss. The immunomodulatory-role of LR was further supported by serum-cytokine data with a significant reduction in proinflammatory-cytokines (IL-6, IL-17 and TNF-α) along with enhancement in anti-inflammatory-cytokines (IL-10, IFN-γ) in LR treated-group. Altogether, the present study for the first time establishes the osteoprotective role of LR on bone-health, thus highlighting the potential of LR in the treatment and management of various bone related diseases including osteoporosis.
Intracellular nanovesicles mediate integrin trafficking during cell migration
Authors: Larocque Gabrielle , La-Borde Penelope J. , Wilson Beverley J. , Clarke Nicholas I. , Moore Daniel J. , Caswell Patrick T. , Royle Stephen J.
Published: August 2020

Membrane traffic is an important regulator of cell migration through the endocytosis and recycling of cell surface receptors such as integrin heterodimers. Intracellular nanovesicles (INVs), are a recently identified class of transport vesicle that are involved in multiple membrane trafficking steps including the recycling pathway. The only known marker for INVs is Tumor Protein D54 (TPD54/TPD52L2), a member of the TPD52-like protein family. Overexpression of TPD52-like family proteins in cancer has been linked to poor prognosis and an aggressive metastatic phenotype which suggests cell migration may be altered under these conditions. Here we show that TPD54 associates with INVs by directly binding high curvature membrane via a conserved positively charged motif in its C-terminus. We describe how other members of the TPD52-like family are also associated with INVs and we document the Rab GTPase complement of all INVs. Depletion of TPD52-like proteins inhibits cell migration and invasion; and we show that this is likely due to altered integrin recycling. Our study highlights the involvement of INVs in the trafficking of cell surface proteins to generate biologically important outputs in health and disease.
Global population genomic analysis ofMycoplasma bovisisolates reveals transcontinental variations and potential virulence genes
Authors: Kumar Roshan , Register Karen , Christopher-Hennings Jane , Moroni Paolo , Gioa Gloria , Garcia-Fernandez Nuria , Nelson Julia , Jelinski Murray , Lysnyansky Inna , Bayles Darrell , Alt David , Scaria Joy
Published: August 2020

Among more than twenty species belonging to the class Mollecutes,Mycoplasma bovisis the most common cause of bovine mycoplasmosis in North America and Europe. Bovine mycoplasmosis causes significant economic loss in the cattle industry. The number ofM. bovispositive herds has recently increased in North America and Europe. Since antibiotic treatment is ineffective and no efficient vaccine is available,M. bovis-induced mycoplasmosis is primarily controlled by herd management measures such as the restriction of moving infected animals out of the herds and culling of infected animals or shedders. To better understand the population structure and genomic factors that may contribute to its transmission, we sequenced 147M. bovisstrains isolated from four different countries and hosts, primarily cattle. We performed a large-scale comparative analysis ofM. bovisgenomes by integrating 104 publicly available genomes and our dataset (251 total genomes). A whole genome-single nucleotide polymorphism (SNP)-based phylogeny revealed thatM. bovispopulation structure is composed of five clades with one of the isolates clustering with the outgroupM. agalactiae. These isolates were found to cluster with those from Canada, Israel, Lithuania, and Switzerland, suggesting trans-continental transmission of the strains. We also validated a previous report suggesting minimum divergence in isolates of Australian origin, which grouped within a single clade along with strains from China and Israel. However, no observable pattern of host association inM. bovisgenomes was found in this study. Our comparative genome analysis also revealed thatM. bovishas an open pangenome with a large breadth of unexplored diversity of genes. Analysis ofvspgene-host association revealed a singlevspsignificantly associated with bovine isolates that may be targeted for diagnostics or vaccine development. Our study also found thatM. bovisgenome harbors a large number of IS elements, including a novel 1624 bp IS element, and ISMbov9. Collectively, the genome data and the whole genome-based population analysis in this study may help to develop control measures to reduce the incidence ofM. bovis-induced mycoplasmosis in cattle and/or to identify candidate genes for vaccine development.
Detection of simple and complex de novo mutations with multiple reference sequences
Authors: Garimella Kiran V. , Iqbal Zamin , Krause Michael A. , Campino Susana , Kekre Mihir , Drury Eleanor , Kwiatkowski Dominic , Sá Juliana M. , Wellems Thomas E. , McVean Gil
Published: August 2020

The characterization of de novo mutations in regions of high sequence and structural diversity from whole-genome sequencing data remains highly challenging. Complex structural variants tend to arise in regions of high repetitiveness and low complexity, challenging both de novo assembly, in which short reads do not capture the long-range context required for resolution, and mapping approaches, in which improper alignment of reads to a reference genome that is highly diverged from that of the sample can lead to false or partial calls. Long-read technologies can potentially solve such problems but are currently unfeasible to use at scale. Here we present Corticall, a graph-based method that combines the advantages of multiple technologies and prior data sources to detect arbitrary classes of genetic variant. We construct multisample, colored de Bruijn graphs from short-read data for all samples, align long-read–derived haplotypes and multiple reference data sources to restore graph connectivity information, and call variants using graph path-finding algorithms and a model for simultaneous alignment and recombination. We validate and evaluate the approach using extensive simulations and use it to characterize the rate and spectrum of de novo mutation events in 119 progeny from four Plasmodium falciparum experimental crosses, using long-read data on the parents to inform reconstructions of the progeny and to detect several known and novel nonallelic homologous recombination events.
The XFM beamline at the Australian Synchrotron
Authors: Howard Daryl L. , de Jonge Martin D. , Afshar Nader , Ryan Chris G. , Kirkham Robin , Reinhardt Juliane , Kewish Cameron M. , McKinlay Jonathan , Walsh Adam , Divitcos Jim , Basten Noel , Adamson Luke , Fiala Tom , Sammut Letizia , Paterson David J.
Published: August 2020

The X-ray fluorescence microscopy (XFM) beamline is an in-vacuum undulator-based X-ray fluorescence (XRF) microprobe beamline at the 3 GeV Australian Synchrotron. The beamline delivers hard X-rays in the 4–27 keV energy range, permitting K emission to Cd and L and M emission for all other heavier elements. With a practical low-energy detection cut-off of approximately 1.5 keV, low-Z detection is constrained to Si, with Al detectable under favourable circumstances. The beamline has two scanning stations: a Kirkpatrick–Baez mirror microprobe, which produces a focal spot of 2 µm × 2 µm FWHM, and a large-area scanning `milliprobe', which has the beam size defined by slits. Energy-dispersive detector systems include the Maia 384, Vortex-EM and Vortex-ME3 for XRF measurement, and the EIGER2 X 1 Mpixel array detector for scanning X-ray diffraction microscopy measurements. The beamline uses event-mode data acquisition that eliminates detector system time overheads, and motion control overheads are significantly reduced through the application of an efficient raster scanning algorithm. The minimal overheads, in conjunction with short dwell times per pixel, have allowed XFM to establish techniques such as full spectroscopic XANES fluorescence imaging, XRF tomography, fly scanning ptychography and high-definition XRF imaging over large areas. XFM provides diverse analysis capabilities in the fields of medicine, biology, geology, materials science and cultural heritage. This paper discusses the beamline status, scientific showcases and future upgrades.
Deep learning enables the atomic structure determination of the Fanconi Anemia core complex from cryoEM
Authors: Farrell Daniel P. , Anishchenko Ivan , Shakeel Shabih , Lauko Anna , Passmore Lori A. , Baker David , DiMaio Frank
Published: August 2020

Cryo-electron microscopy of protein complexes often leads to moderate resolution maps (4–8 Å), with visible secondary-structure elements but poorly resolved loops, making model building challenging. In the absence of high-resolution structures of homologues, only coarse-grained structural features are typically inferred from these maps, and it is often impossible to assign specific regions of density to individual protein subunits. This paper describes a new method for overcoming these difficulties that integrates predicted residue distance distributions from a deep-learned convolutional neural network, computational protein folding using Rosetta, and automated EM-map-guided complex assembly. We apply this method to a 4.6 Å resolution cryoEM map of Fanconi Anemia core complex (FAcc), an E3 ubiquitin ligase required for DNA interstrand crosslink repair, which was previously challenging to interpret as it comprises 6557 residues, only 1897 of which are covered by homology models. In the published model built from this map, only 387 residues could be assigned to the specific subunits with confidence. By building and placing into density 42 deep-learning-guided models containing 4795 residues not included in the previously published structure, we are able to determine an almost-complete atomic model of FAcc, in which 5182 of the 6557 residues were placed. The resulting model is consistent with previously published biochemical data, and facilitates interpretation of disease-related mutational data. We anticipate that our approach will be broadly useful for cryoEM structure determination of large complexes containing many subunits for which there are no homologues of known structure.
Characterization and structure of glyceraldehyde-3-phosphate dehydrogenase type 1 from Escherichia coli
Authors: Zhang L. , Liu M. R. , Yao Y. C. , Bostrom I. K. , Wang Y. D. , Chen A. Q. , Li J. X. , Gu S. H. , Ji C. N.
Published: August 2020

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a key enzyme in the glycolytic pathway that catalyzes the conversion of D-glyceraldehyde 3-phosphate to 1,3-diphosphoglycerate. Here, the full-length GAPDH type 1 from Escherichia coli (EcGAPDH1) was cloned and overexpressed, and the protein was purified. Biochemical analyses found that the optimum reaction temperature and pH of EcGAPDH1 were 55°C and 10.0, respectively. The protein has a certain amount of thermostability. Crystals of EcGAPDH1 were obtained using the sitting-drop vapor-diffusion technique and X-ray diffraction data were collected to 1.88 Å resolution. Characterization of the crystals showed that they belonged to space group P41212, with unit-cell parameters a = b = 89.651, c = 341.007 Å, α = β = γ = 90°. The structure of EcGAPDH1 contains four subunits, each of which includes an N-terminal NAD+-binding domain and a C-terminal catalytic domain. Analysis of the NAD+-bound form showed some differences between the structures of EcGAPDH1 and human GAPDH. As EcGAPDH1 shares 100% identity with GAPDH from Shigella sonnei, its structure may help in finding a drug for the treatment of shigellosis.
Venture capital network and the M&A performance of listed companies
Authors: Jin Yonghong , Xu Meng , Wang Wei , Xi Yuqin
Published: August 2020

Purpose

The purpose of this paper is to discuss how venture capital institutions can use their syndicated investment network to help listed companies to achieve better performance in mergers and acquisitions (M&A) activities.

Design/methodology/approach

This paper builds a fixed effect unbalanced panel regression model to study the impact of venture capital network on the M&A performance of listed companies.

Findings

Evidence indicated that the stronger the information resource acquisition ability of venture capital institutions in the network, the better the listed company's M&A performance supported; the stronger the information resource control ability of venture capital institutions in the network, the better the listed company's M&A performance supported; the higher the participation of venture capital institutions, the more significant the positive impact of information resource acquisition and information resource control abilities on M&A performance in the network.

Research limitations/implications

The data in this paper are from China's Growth Enterprise Market (GEM), other markets may be considered in the future research studies.

Practical implications

The research conclusions of this paper affirm the positive role played by venture capital institutions through syndicated investment in eliminating information asymmetry in M&A of invested companies. The information resource acquisition and control abilities and participation degree of the venture capital network have positively promoted the M&A performance of the invested enterprises.

Originality/value

The conclusions of this paper not only provide useful supplements to existing research literature on venture capital network functions and corporate M&A but also have certain guiding value for venture capital institutions and start-ups to better use venture capital practices to improve their capabilities and performance.
Examining strategies behind universities’ technology transfer portfolio: how different patterns of resource consumption can lead to similar technology transfer profiles
Authors: Berbegal-Mirabent Jasmina , Gil-Doménech Dolors , de la Torre Eva M.
Published: August 2020

Purpose

The purpose of this study is to analyse how different patterns of production factors consumption of Spanish universities lead to specific technology transfer (TT) profiles (outcomes).

Design/methodology/approach

Adopting a resource-based view perspective (RBV), qualitative comparative analysis (QCA) is used to analyse the relationship between different combinations of resources – human resources, financial resources and support services – and various portfolios of TT outcomes – intellectual or industrial property agreements, spin-offs and TT income.

Findings

Results indicate that there is no unique formula of resource consumption that leads to a specific portfolio of TT outcomes. These results seem to reflect the characteristics and competencies added by universities, along with the characteristics of their socioeconomic context. From a RVB perspective, this indicates that the considered resources are substitutable.

Practical implications

The effectiveness of university policies is expected to vary by university, for example depending on the type of resources that is most relevant in the university’s production process. To develop competitive advantages Spanish public universities must resort to internal intangible resources or specific and inimitable combinations of the available resources.

Social implications

Since Spanish universities are heterogeneous and display different TT portfolios they address the needs of different users.

Originality/value

Previous studies have failed to acknowledge the heterogeneity among universities. To perform the analysis QCA is used, an innovative methodology in the higher education sector that enables us to purposefully acknowledge institutional diversity (in both resources and results). This allows us to indirectly take into account the capabilities of universities using a more holistic approach to evaluate their competitiveness.
From green buildings to living buildings? Rating schemes and waste management practices in Australian educational buildings
Authors: Udawatta Nilupa , Zuo Jian , Chiveralls Keri , Zillante George
Published: August 2020

Purpose

There is an increasing level of recognition of the pressing issues associated with climate change and resource depletion. As a result, it is well recognised that higher education institutions bear responsibilities to promote “sustainable development”. Many universities have adopted green building practices in the construction of their building infrastructure. A variety of Green Building Rating Tools (GBRTs) have been designed to facilitate green building developments. Thus, the aim of this research is to identify mechanisms to improve current GBRTs in terms of waste management (WM) practices by using green star accredited educational buildings in Australia.

Design/methodology/approach

A qualitative approach was adopted in this study to achieve the research aim by conducting three case studies of educational buildings in South Australia. Thirty three interviews were carried out in a face-to-face, semi-structured manner and project documentations were reviewed. The participants were asked to provide their expert opinions on the GS initiative and its ability to minimise waste generation, the impact of the GS initiative on solid WM practices and problems associated with the implementation process of the GS initiative. Data was analysed using code-based content analysis using the NVivo software package. Tables and figures were used as the visualization technique to present an expedient understanding in a holistic manner.

Findings

Findings showed that the Green Star (GS) initiative drives change in the way current practices are performed in the Australian construction industry. However, this study revealed that WM targets outlined in the GS initiative are not challenging enough. Thus, suggestions are provided in this research to improve the WM aspects of GS initiatives by looking beyond a focus on “sustainability” and “waste minimisation” towards a focus on regenerative environments.

Originality/value

These findings are valuable for practitioners and policymakers seeking to improve WM practices and to address issues associated with climate change and resource depletion.
Sunscreen use: a theory-based interventional study using HAPA
Authors: Panahi Hadiseh , Keikavoosi-Arani Leila , Salehi Leili
Published: August 2020

Purpose

The current study was aimed to assess health action process approach (HAPA) effectiveness in sunscreen using among paddy workers

Design/methodology/approach

This interventional study was conducted on 177 paddy workers from Rudsar city. The subjects were randomly assigned into motivational, volitional and control groups. The motivational and volitional groups received the educational intervention based on HAPA. Three groups were evaluated in terms of HAPA constructs, intention and behavior at three times before the intervention, immediately afterward and one month later. Chi-square, repeated measure test were used to analyze the data using SPSS software version 19.

Findings

The mean age was 47.78 ± 12.66. The majority of the participants were female (69.3%) and had diploma. based on repeated measure test results the score of the use of sunscreen during the time in intervention groups as well as between the three groups were changed. Based on the results of this study, the score of the constructs of both phases of HAPA in the two groups (motivational and volitional) compared to control group was significantly improved (p < 0.05).

Practical implications

The study shows the use of HAPA for the behavioral change related to sunscreen use among the paddy workers as the high-risk group.

Originality/value

North of Iran
Identifying key quality features for wearable technology embedded products using the Kano model
Authors: Salahuddin Mir , Lee Young-A
Published: August 2020

Purpose

The purpose of this study was to identify the major quality features of wearable technology embedded products that have the greatest impact on consumer satisfaction using the Kano model, an organized approach to specify consumer requirements and expectation through a preference classification technique.

Design/methodology/approach

Using a quantitative research method, an online survey was conducted with a convenience sample of US consumers aged between 19 years old and over. A total of otal 471 useable data were obtained and used for the data analysis.

Findings

This study identified that the 11 quality features of wearables belong to one-dimensional quality category among the five Kano categories, although the impact of each quality feature's performance on consumer satisfaction varies. The results also showed that the performance level of durability, long battery life, usability, product safety, comfortability and reasonable price has the greatest impact on consumer satisfaction of wearables.

Research limitations/implications

This study has implications for future research by integrating the Kano model with other design and product development related theoretical models when designing, developing and evaluating various wearable products.

Originality/value

This study quantified the key quality features of wearables using the Kano model, which can be a great measurement tool for future researchers to adopt in their studies. The findings of this study help designers, developers and producers of wearables to prioritize the quality features during the product design, development and manufacturing process.
Moderating effect of optimum stimulation level on the relationship between satisfaction and revisit intention: the case of Turkish cultural tourists
Authors: Evren Savaş , Şimşek Evren Emine , Çakıcı A. Celil
Published: August 2020

Purpose

The purpose of this study is to determine the optimum stimulation levels (OSLs) of cultural tourists within the context of their novelty seeking tendencies and to determine whether their OSLs have a moderating effect on the relationship between their satisfaction and revisit intentions.

Design/methodology/approach

To collect data, an online survey was conducted for Turkish cultural tourists (n = 563) in May 2018, and the data were analysed with PROCESS by Hayes.

Findings

The study’s findings demonstrated that Turkish cultural tourists were high-level novelty seekers and that novelty seeking has a moderating effect on the relationship between their satisfaction and short-term revisit intentions.

Originality/value

This study contributes to the literature by revealing the moderator effect of novelty seeking tendency on the relationship between satisfaction and revisit intention within the scope of cultural tourism, which has not been examined before.
The politics behind design projects: when space, organization, and technology collide
Authors: Jemine Grégory , Pichault François , Dubois Christophe
Published: August 2020

Purpose

While more and more organizations commit to transformation projects with the aim of redesigning simultaneously their workspaces, work organization, and technologies, the design process supporting such projects remains largely understudied. This paper examines the political tensions that occur when such processes unfold as well as their implications for project management. By doing so, the paper counterbalances the prescriptive and normative literature on “New Ways of Working” which largely overlooks the political complexity of such projects.

Design/methodology/approach

The paper is based on a qualitative study of a triple design process in a media company. Data collection mainly consists of a nine-month process of non-participant observation of weekly meetings held by the strategic group in charge of the project. Semi-structured interviews with members of the executive committee have also been conducted.

Findings

The analysis illustrates how space, organization and technology are gradually designed and structured. Four interconnected and often concealed mechanisms that support triple design processes are identified: political tensions, unexpected twists, conflicting temporalities and arbitration measures.

Originality/value

The originality of the paper lies in breaking down the concept of design in three separate objects – organization, space and technology – and examining how these objects were conjointly problematized by an organization in transformation, whereas existing studies often investigate organization design, space design or technology design in isolation.
New product development process in apparel industry using Industry 4.0 technologies
Authors: Wijewardhana Ganguli Eranga Harshamali , Weerabahu Samanthi Kumari , Nanayakkara Julian Liyanage Don , Samaranayake Premaratne
Published: August 2020

Purpose

The main purpose of this paper is to assess the technological view of apparel new product development (NPD) process through a framework for improving the efficiency of NPD process in Sri Lankan apparel industry.

Design/methodology/approach

The study uses literature review, a survey and in-depth interviews of industry experts for data collection. Descriptive analysis and correlation analysis are used to identify the relationship between NPD process performance and Industry 4.0 (I4) technology components.

Findings

All technology components are enablers of NPD process, yet the relative importance of technology components varies within the NPD life cycle. Technoware is identified as the most significant, while Humanware component is the least significant for the advancement of I4 technologies in the NPD process.

Research limitations/implications

Despite the novelty of research investigation into NPD process using I4 technologies in apparel industry, it is limited to one geographical location and a small segment of the industry.

Practical implications

This paper assists apparel industry practitioners to better understand and prioritize I4 technology components in the NPD life cycle for their successful adoption and for reaping the benefits.

Originality/value

This research generates new knowledge on the adoption of I4 technologies using industry insights into technology components mapped/aligned with key stages of NPD life cycle.
What influences patients' willingness to choose in online health consultation? An empirical study with PLS–SEM
Authors: Wan Yan , Zhang Yifan , Yan Mengling
Published: August 2020

Purpose

Online trust is a key factor for successful online transactions. To some extent, online health consultation can be considered an online transaction. Owing to the unique physician–patient relationship, patients' choice of physicians within an online health consultation setting may not only be based on rational judgments but also considerably affected by their feelings. Hence, the purpose of this study was to explore which physicians' attributes affect patients' cognitive and affective trust in physicians, as well as how these two variables and their association affect patients' willingness to choose.

Design/methodology/approach

Based on the broad conceptual framework of online trust, this paper proposed a set of hypotheses that were tested through analyzing survey data using structural equation modeling techniques.

Findings

Results showed that physicians' ability had a significant positive influence on patients' cognitive trust in the physician; physicians' integrity and benevolence had a significant positive influence on patients' affective trust in the physician; cognitive and affective trust had a significant positive impact on patients' willingness to choose and there were significant positive interactions between affective and cognitive trust.

Originality/value

This study theoretically enriches the generic model of online trust. From the practical perspective, it will provide physicians working in online health consultation platforms and relevant practitioners with baseline information on the topic and advice for decision-making toward service enhancement and clientele improvements.
Do value cocreation and engagement drive brand evangelism?
Authors: Harrigan Paul , Roy Sanjit K. , Chen Tom
Published: August 2020

Purpose

Drawing on service logic, the authors investigate how value cocreation leads to evangelical brand-related behaviors (brand defense and brand advocacy). The authors analyze the interplay between value cocreation and customer brand engagement on social media in driving these outcomes. The authors also consider the role of brand love in eliciting evangelical brand-related behaviors.

Design/methodology/approach

Respondents recruited through Amazon MTurk were surveyed on social media use in tourism-related decisions. The total useable sample size was 397. Partial least squares structural equation modeling (PLS-SEM) was used to test the research model.

Findings

Value cocreation and customer brand engagement are drivers of evangelical brand-related behaviors, emphasizing the importance of these two in marketing and how they drive behavioral outcomes.

Research limitations/implications

Service logic highlights the significance of value cocreation which, through customer brand engagement and love, leads to brand defense and advocacy. This explains the mediation in our model, where marketers must undertake efforts to support customer brand engagement and brand love.

Practical implications

Value is created by the user for the user through their experiences over time. Brands are owned by customers, and their defense and advocacy of them must be earned. Marketers facilitate customer value creation by providing the resources to cocreate value and love the brand.

Originality/value

Most studies investigate value cocreation from an in-role and/or extra-role perspective as to how it benefits firms. Through service logic, the authors illustrate how it leads to evangelical brand-related behaviors.
Characterization of the Relationship of Inotuzumab Ozogamicin Exposure With Efficacy and Safety End Points in Adults With Relapsed or Refractory Acute Lymphoblastic Leukemia
Authors: Chen Joseph , Haughey May , Vandendries Erik , DeAngelo Daniel J. , Kantarjian Hagop M. , Ruiz‐Garcia Ana
Published: August 2020

Inotuzumab ozogamicin (InO), an anti‐CD22 monoclonal antibody conjugated to calicheamicin, is approved in Europe and the United States for treatment of adults with relapsed or refractory acute lymphoblastic leukemia (ALL). Population analyses were performed to evaluate the relationship between InO exposure and efficacy and safety end points in patients with ALL. The probability of achieving complete remission/complete remission with incomplete hematologic recovery (CR/CRi) and minimal residual disease (MRD)‐negativity for InO relative to chemotherapy was also investigated. Data from study 1010 (NCT01363297) and INO‐VATE (NCT01564784) were pooled for exposure–response (InO, n = 234) and treatment–response (InO, n = 234; chemotherapy, n = 143) analyses. The analyses demonstrated that InO exposure was significantly correlated with achieving CR/CRi and MRD‐negativity, as well as with hepatic event adjudication board‐reported veno‐occlusive disease/sinusoidal obstruction. Patients with ALL treated with InO had significantly greater odds of achieving CR/CRi (7‐times higher) and MRD‐negativity (13‐times higher) than those receiving chemotherapy.
Knowledge, attitudes, and beliefs regarding molar incisor hypomineralization (MIH) amongst German dental students
Authors: Elhennawy Karim , Anang Mostafa , Splieth Christian , Bekes Katrin , Manton David John , Hedar Zen , Krois Joachim , Jost‐Brinkmann Paul‐Georg , Schwendicke Falk
Published: August 2020

Background

Knowledge of molar incisor hypomineralization (MIH) has relevance for paediatric dentists.

Aim

To assess final‐year German dental students’ knowledge, attitudes, and beliefs regarding MIH.

Materials and methods

A previously validated questionnaire was posted to the 31 German dental schools. Demographic covariates as well as knowledge regarding

diagnosis and prevalence, and attitudes and beliefs around aetiology and management were collected.

Results

Twenty‐two (71%) dental schools responded and a total of 877 students participated. Most (97%) were familiar with MIH and 88% were aware of the diagnostic criteria for MIH; however, only 42% knew how to implement them. One‐third were able to identify MIH and 16% reported diagnostic confidence when doing so; 90% assumed the MIH prevalence to be <10%. Two‐thirds of the respondents implicated genetic components as the main aetiological factor of MIH. Resin composite (60%) and preformed metal crowns (46%) were the dental materials most often suggested for restorative management. Almost all (98%) respondents were interested in receiving more clinical training.

Conclusion

German students were familiar with MIH; however, they reported low levels of knowledge and confidence regarding its prevalence and diagnosis. Standardized nationwide, up‐to‐date curricula should be implemented to educate future dentists in Germany.
Use of dementia care mapping in the care for older people with intellectual disabilities: A mixed‐method study
Authors: Schaap Feija D. , Dijkstra Geke J. , Reijneveld Sijmen A. , Finnema Evelyn J.
Published: August 2020

Background

The ageing of people with intellectual disabilities, with associated morbidity like dementia, calls for new types of care. Person‐centred methods may support care staff in providing this, an example being Dementia Care Mapping (DCM). DCM has been shown to be feasible in ID‐care. We examined the experiences of ID‐professionals in using DCM.

Methods

We performed a mixed‐methods study, using quantitative data from care staff (N = 136) and qualitative data (focus‐groups, individual interviews) from care staff, group home managers and DCM‐in‐intellectual disabilities mappers (N = 53).

Results

DCM provided new insights into the behaviours of clients, enabled professional reflection and gave new knowledge and skills regarding dementia and person‐centred care. Appreciation of DCM further increased after the second cycle of application.

Conclusion

DCM is perceived as valuable in ID‐care. Further assessment is needed of its effectiveness in ID‐care with respect to quality of care, staff‐client interactions and job performance.
Long non‐coding RNA MALAT1 targeting STING transcription promotes bronchopulmonary dysplasia through regulation of CREB
Authors: Chen Jia‐He , Feng Dan‐Dan , Chen Yu‐Fei , Yang Cai‐Xia , Juan Chen‐Xia , Cao Qian , Chen Xi , Liu Shuang , Zhou Guo‐Ping
Published: August 2020

Bronchopulmonary dysplasia (BPD) is a severe complication of preterm infants characterized by increased alveolarization and inflammation. Premature exposure to hyperoxia is believed to be a key contributor to the pathogenesis of BPD. No effective preventive or therapeutic agents have been created. Stimulator of interferon gene (STING) is associated with inflammation and apoptosis in various lung diseases. Long non‐coding RNA MALAT1 has been reported to be involved in BPD. However, how MALAT1 regulates STING expression remains unknown. In this study, we assessed that STING and MALAT1 were up‐regulated in the lung tissue from BPD neonates, hyperoxia‐based rat models and lung epithelial cell lines. Then, using the flow cytometry and cell proliferation assay, we found that down‐regulating of STING or MALAT1 inhibited the apoptosis and promoted the proliferation of hyperoxia‐treated cells. Subsequently, qRT‐PCR, Western blotting and dual‐luciferase reporter assays showed that suppressing MALAT1 decreased the expression and promoter activity of STING. Moreover, transcription factor CREB showed its regulatory role in the transcription of STING via a chromatin immunoprecipitation. In conclusion, MALAT1 interacts with CREB to regulate STING transcription in BPD neonates. STING, CREB and MALAT1 may be promising therapeutic targets in the prevention and treatment of BPD.
Boundary Layer Ingestion Propulsion: A Review on Numerical Modeling
Authors: Menegozzo Luca , Benini Ernesto
Published: August 2020

The present paper focuses on the numerical modeling approaches adopted in boundary layer ingestion (BLI) studies. Three driving aircraft concepts have been identified, namely propulsive fuselage concept (PFC), rear engines concept (REC), and distributed fans concept (DFC). The affiliation to relevant research projects has been considered. Specifically, European projects DisPURSAL and CENTRELINE, NASA projects STARC-ABL, D8, and N3-X, as well as ONERA projects NOVA and DRAGON have been examined, together with other significant works. The methodologies adopted by the reviewed analyses have been investigated and summarized for each concept, in order to assess the main trends of BLI modeling strategies.
Development, survival and reproduction of a potential biological control agent, Lasioseius japonicus Ehara (Acari: Blattisociidae), on eggs of Drosophila melanogaster (Diptera: Drosophilidae) and Sitotroga cerealella (Lepidoptera: Gelechiidae)

Authors: Xie Lixia , Zhang Na , Wu Xinran , Liu Kai , Liu Chenlin , Yan Yi
Published: August 2020

Lasioseius japonicus is a species of soil-dwelling predatory mite with great potential for controlling insect pests and has a broad application prospect in biological control. This study was conducted under indoor conditions to explore the development, survival and reproduction of L. japonicus on two diets. Through the construction of two-sex life table of L. japonicus, it was revealed that, when it preyed on eggs of Drosophila melanogaster, the intrinsic rate of increase (r) was 0.2614 d-1, the net reproductive rate (R0) was 57.76 offspring, the finite rate of increase (λ) was 1.2988 d-1, and the mean generation time (T) was 15.49 d. When preying on eggs of Sitotroga cerealella, the intrinsic rate of increase (r) of L. japonicus was 0.1560 d-1, the net reproductive rate (R0) was 20.96 offspring, the finite rate of increase (λ) was 1.1690d-1, and the mean generation time (T) was 19.41 d. In addition, the life expectancy and population growth trend of L. japonicus were simulated based on the above two alternative prey species. The results showed that feeding on the eggs of D. melanogaster is more beneficial to the growth and reproduction of L. japonicus.
Low-energy high-flux ion bombardment-induced interfacial mixing during Al2O3 plasma-enhanced atomic layer deposition
Authors: Hirayama Masaki , Sugawa Shigetoshi
Published: August 2020

We investigated the formation mechanism of interfacially mixed AlSiOx films on Si surfaces under low-energy (<15 eV), high-flux (>4 × 1015 cm−2 s−1) O2+ ion bombardment during Al2O3 plasma-enhanced atomic layer deposition. When high-dose O2+ ions (>1 × 1017 cm−2 cycle−1) were incident on the growing film surface during the oxidation steps in Al2O3 atomic layer deposition (ALD), interfacially mixed films with thin (∼0.8 nm) SiOx interlayers were formed on Si surfaces. The deposited films possessed a layered structure of Al2O3/AlSiOx/AlOx layers. In order to reveal the formation mechanism, films deposited during the early stages of ALD were evaluated using an angle-resolved x-ray photoelectron spectroscope. In the first oxidation step, thin, low-density SiOx films with weak Si–O bonds containing excess oxygen were formed on the Si surface. In subsequent oxidation steps, adsorbed Al atoms on the surface were knocked on by the energetic ions and penetrated into the SiOx film. As a result, Al atoms were taken into SiOx. Some of the Al atoms accumulated near the interface. After several tens of cycles, ordinary Al2O3 films were deposited on the Si-rich layers. Thus, the films with a layered structure of Al2O3/AlSiOx/AlOx layers were formed on the Si substrate.
Seabed classification using physics-based modeling and machine learning
Authors: Frederick Christina , Villar Soledad , Michalopoulou Zoi-Heleni
Published: August 2020

In this work, model-based methods are employed, along with machine learning techniques, to classify sediments in oceanic environments based on the geoacoustic properties of a two-layer seabed. Two different scenarios are investigated. First, a simple low-frequency case is set up, in which the acoustic field is modeled with normal modes. Four different hypotheses are made for seafloor sediment possibilities, and these are explored using both various machine learning techniques and a simple matched-field approach. For most noise levels, the latter has an inferior performance to the machine learning methods. Second, the high-frequency model of the scattering from a rough, two-layer seafloor is considered. Again, four different sediment possibilities are classified with machine learning. For higher accuracy, one-dimensional convolutional neural networks are employed. In both cases, the machine learning methods, both in simple and more complex formulations, lead to effective sediment characterization. The results assess the robustness to noise and model misspecification of different classifiers.
Structure and assembly of ESCRT-III helical Vps24 filaments
Authors: Huber Stefan T. , Mostafavi Siavash , Mortensen Simon A. , Sachse Carsten
Published: August 2020

The cryo-EM structure of Vps24 homopolymer filaments reveals the structural basis for ESCRT-III lipid assemblies.
Structural batteries take a load off
Authors: Lutkenhaus Jodie L. , Flouda Paraskevi
Published: August 2020

Multifunctional Zn-air batteries provide energy storage and a body-integrated protective cover for robots.
Degradation of 5hmC-marked stalled replication forks by APE1 causes genomic instability
Authors: Kharat Suhas S. , Ding Xia , Swaminathan Divya , Suresh Akshey , Singh Manish , Sengodan Satheesh K. , Burkett Sandra , Marks Hanna , Pamala Chinmayi , He Yafeng , Fox Stephen D. , Buehler Eugen C. , Muegge Kathrin , Martin Scott E. , Sharan Shyam K.
Published: August 2020

Loss of DNA demethylase TET2 protects stalled replication forks from degradation and confers chemoresistance.
Considering COVID-19 kidney injury
Authors: Nadkarni Girish N.
Published: August 2020

Does SARS-CoV-2 infection have long-term effects on the kidney?
Detection of SARS-CoV-2 IgG Targeting Nucleocapsid or Spike Protein by Four High-Throughput Immunoassays Authorized for Emergency Use
Authors: Prince Harry E. , Givens Tara S. , Lapé-Nixon Mary , Clarke Nigel J. , Schwab Dale A. , Batterman Hollis J. , Jones Robert S. , Meyer William A. , Kapoor Hema , Rowland Charles M. , Haji-Sheikhi Farnoosh , Marlowe Elizabeth M.
Published: August 2020

A total of 1,200 serum samples that were tested for SARS-CoV-2 IgG antibody using the Abbott Architect immunoassay targeting the nucleocapsid protein were run in 3 SARS-CoV-2 IgG immunoassays targeting spike proteins (DiaSorin Liaison, Ortho Vitros, and Euroimmun). Consensus-positive and consensus-negative interpretations were defined as qualitative agreement in at least 3 of the 4 assays. Agreement of the 4 individual assays with a consensus-negative interpretation (
n
 = 610) ranged from 96.
Genome Sequences of the Mycobacteriophages Awesomesauce and LastJedi
Authors: Davis Madison E. , Delesalle Véronique A. , Blankenship Kayla D. , Good Jeffrey T. , Kroenke Clayr M. , Kuba Gabrielle M. , Sweeney Sean P. , Byrum Christine A.
Published: August 2020

Bacteriophages Awesomesauce and LastJedi infect
Mycobacterium smegmatis
mc
2
155. While the Awesomesauce genome is 57,054 bp with 94 protein-coding genes, the LastJedi genome is 55,149 bp with 94 protein-coding genes. Nucleotide sequence comparison in Phamerator detected synteny between Awesomesauce gp49 to gp61 and singleton LilSpotty. Whole-genome BLASTn alignments revealed that LastJedi strongly resembles Clifton (99.41% identity).