bluuebunny/crossref_metadata_2025 huggingface dataset:
Machine and Deep Learning Trends in EEG-Based Detection and Diagnosis of Alzheimer’s Disease: A Systematic Review
Authors: Aviles Marcos , Sánchez-Reyes Luz María , Álvarez-Alvarado José Manuel , Rodríguez-Reséndiz Juvenal
Published: July 2024

This article presents a systematic review using PRISMA methodology to explore trends in the use of machine and deep learning in diagnosing and detecting Alzheimer’s disease using electroencephalography. This review covers studies published between 2013 and 2023, drawing on three leading academic databases: Scopus, Web of Science, and PubMed. The validity of the databases is evaluated considering essential factors such as the arrangement of EEG electrodes, data acquisition methodologies, and the number of participants. Additionally, the specific properties of the databases used in the research are highlighted, including EEG signal classification, filtering, segmentation approaches, and selected features. Finally, the performance metrics of the classification algorithms are evaluated, especially the accuracy achieved, offering a comprehensive view of the current state and future trends in the use of these technologies for the diagnosis of Alzheimer’s disease.
Mass Spectrometry Characterization of Honeydew Honey: A Critical Review
Authors: Quirantes-Piné Rosa , Sanna Gavino , Mara Andrea , Borrás-Linares Isabel , Mainente Federica , Picó Yolanda , Zoccatelli Gianni , Lozano-Sánchez Jesús , Ciulu Marco
Published: July 2024

Honeydew honey is produced by bees (Apis mellifera) foraging and collecting secretions produced by certain types of aphids on various parts of plants. In addition to exhibiting organoleptic characteristics that distinguish them from nectar honey, these honeys are known for their functional properties, such as strong antioxidant and anti-inflammatory activities. Despite their importance, they remain poorly characterized in comparison with flower honeys, as most studies on this subject are not only carried out on too few samples but also still focused on traditional chemical–physical parameters, such as specific rotation, major sugars, or melissopalynological information. Since mass spectrometry has consistently been a primary tool for the characterization and authentication of honeys, this review will focus on the application of these methods to the characterization of the minor fraction of honeydew honey. More specifically, this review will attempt to highlight what progress has been made so far in identifying markers of the authenticity of the botanical and/or geographical origin of honeydew honeys by mass spectrometry-based approaches. Furthermore, strategies devoted to the determination of contaminants and toxins in honeydew honeys will be addressed. Such analyses represent a valuable tool for establishing the level of food safety associated with these products. A critical analysis of the presented studies will identify their limitations and critical issues, thereby describing the current state of research on the topic.
The Preparation Phase of the 2022 ML 5.7 Offshore Fano (Italy) Earthquake: A Multiparametric–Multilayer Approach
Authors: Orlando Martina , De Santis Angelo , De Caro Mariagrazia , Perrone Loredana , A. Campuzano Saioa , Cianchini Gianfranco , Piscini Alessandro , D’Arcangelo Serena , Calcara Massimo , Fidani Cristiano , Nardi Adriano , Sabbagh Dario , Soldani Maurizio
Published: July 2024

This paper presents an analysis of anomalies detected during the preparatory phase of the 9 November 2022 ML = 5.7 earthquake, occurring approximately 30 km off the coast of the Marche region in the Adriatic Sea (Italy). It was the largest earthquake in Italy in the last 5 years. According to lithosphere–atmosphere–ionosphere coupling (LAIC) models, such earthquake could induce anomalies in various observable variables, from the Earth’s surface to the ionosphere. Therefore, a multiparametric and multilayer approach based on ground and satellite data collected in each geolayer was adopted. This included the revised accelerated moment release method, the identification of anomalies in atmospheric parameters, such as Skin Temperature and Outgoing Longwave Radiation, and ionospheric signals, such as Es and F2 layer parameters from ionosonde measurements, magnetic field from Swarm satellites, and energetic electron precipitations from NOAA satellites. Several anomalies were detected in the days preceding the earthquake, revealing that their cumulative occurrence follows an exponential trend from the ground, progressing towards the upper atmosphere and the ionosphere. This progression of anomalies through different geolayers cannot simply be attributed to chance and is likely associated with the preparation phase of this earthquake, supporting the LAIC approach.
Experimental Study of Scouring and Deposition Characteristics of Riprap at Embankment Toe Due to Overflow
Authors: Al-Biruni Abu Raihan Mohammad , Billah Md Masum , Yagisawa Junji
Published: July 2024

In this study, the effects of the grain size and gradation of riprap, the overtopping flow depth, and the downstream slope of the embankment on the scouring and deposition characteristics at the downstream toe of the embankment were investigated. For the experiment, three different downstream slopes (1:2, 1:3, and 1:4), three different overflow depths (1, 2, and 3 cm), and three different sizes of riprap particles (d50 of 16.41 mm, 8.48 mm, and 3.39 mm, herein referred to as coarse gravel, medium gravel, and granule, respectively) were used in the laboratory. The experimental results demonstrated that the scour depth and deposition height increased with increasing energy head for each downstream slope condition. Among the three particle sizes, coarse gravel shows the lowest scour depth and the highest deposition height. For the 1:2 slope, the coarse gravel particle size was 62% and 75% less resistant to scouring than the medium gravel and granule particles, respectively. For the 1:3 slope case, this was 31% and 46%, and for the 1:4 slope case, this was 39% and 49% less than the medium gravel and granule size particles, respectively.
Advanced Integration of Urban Street Greenery and Pedestrian Flow: A Multidimensional Analysis in Chengdu’s Central Urban District
Authors: Ma Qicheng , Zhang Jiaxin , Li Yunqin
Published: July 2024

As urbanization accelerates, urban greenery, particularly street greenery, emerges as a vital strategy for enhancing residents’ quality of life, demanding attention for its alignment with pedestrian flows to foster sustainable urban development and ensure urban dwellers’ wellbeing. The advent of diverse urban data has significantly advanced this area of study. Focusing on Chengdu’s central urban district, this research assesses street greening metrics against pedestrian flow indicators, employing spatial autocorrelation techniques to investigate the interplay between street greenery and pedestrian flow over time and space. Our findings reveal a prevalent negative spatial autocorrelation between street greenery and pedestrian flow within the area, underscored by temporal disparities in greenery demands across various urban functions during weekdays versus weekends. This study innovatively incorporates mobile phone signal-based population heat maps into the mismatch analysis of street greenery for the first time, moving beyond the conventional static approach of space syntax topology in assessing pedestrian flow. By leveraging dynamic pedestrian flow data, it enriches our understanding of the disconnect between street greening plans and pedestrian circulation, highlighting the concept of urban flow and delving into the intricate nexus among time, space, and human activity. Moreover, this study meticulously examines multiple street usage scenarios, reflecting diverse behavior patterns, with the objective of providing nuanced and actionable strategies for urban renewal initiatives aimed at creating more inviting and sustainable urban habitats.
RETRACTED: Dong et al. Inhibition of SDF-1α/CXCR4 Signalling in Subchondral Bone Attenuates Post-Traumatic Osteoarthritis. Int. J. Mol. Sci. 2016, 17, 943
Authors: Dong Yonghui , Liu Hui , Zhang Xuejun , Xu Fei , Qin Liang , Cheng Peng , Huang Hui , Guo Fengjing , Yang Qing , Chen Anmin
Published: July 2024

The journal retracts the article “Inhibition of SDF-1α/CXCR4 Signalling in Subchondral Bone Attenuates Post-Traumatic Osteoarthritis” [...]
The Inflammasome Adaptor Protein ASC in Plasma as a Biomarker of Early Cognitive Changes
Authors: Cyr Brianna , Curiel Cid Rosie , Loewenstein David , Vontell Regina T. , Dietrich W. Dalton , Keane Robert W. , de Rivero Vaccari Juan Pablo
Published: July 2024

Dementia is a group of symptoms including memory loss, language difficulties, and other types of cognitive and functional impairments that affects 57 million people worldwide, with the incidence expected to double by 2040. Therefore, there is an unmet need to develop reliable biomarkers to diagnose early brain impairments so that emerging interventions can be applied before brain degeneration. Here, we performed biomarker analyses for apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), neurofilament light chain (NfL), glial fibrillary acidic protein (GFAP), and amyloid-β 42/40 (Aβ42/40) ratio in the plasma of older adults. Participants had blood drawn at baseline and underwent two annual clinical and cognitive evaluations. The groups tested either cognitively normal on both evaluations (NN), cognitively normal year 1 but cognitively impaired year 2 (NI), or cognitively impaired on both evaluations (II). ASC was elevated in the plasma of the NI group compared to the NN and II groups. Additionally, Aβ42 was increased in the plasma in the NI and II groups compared to the NN group. Importantly, the area under the curve (AUC) for ASC in participants older than 70 years old in NN vs. NI groups was 0.81, indicating that ASC is a promising plasma biomarker for early detection of cognitive decline.
Dissection of Metabolome and Transcriptome—Insights into Capsaicin and Flavonoid Accumulation in Two Typical Yunnan Xiaomila Fruits
Authors: Hu Huaran , Du Lei , Zhang Ruihao , Zhong Qiuyue , Liu Fawan , Li Weifen , Gui Min
Published: July 2024

Pepper is an economically important vegetable worldwide, containing various specialized metabolites crucial for its development and flavor. Capsaicinoids, especially, are genus-specialized metabolites that confer a spicy flavor to Capsicum fruits. In this work, two pepper cultivars, YB (Capsicum frutescens L.) and JC (Capsicum baccatum L.) pepper, showed distinct differences in the accumulation of capsaicin and flavonoid. However, the molecular mechanism underlying them was still unclear. Metabolome analysis showed that the JC pepper induced a more abundant accumulation of metabolites associated with alkaloids, flavonoids, and capsaicinoids in the red ripening stages, leading to a spicier flavor in the JC pepper. Transcriptome analysis confirmed that the increased expression of transcripts associated with phenylpropanoid and flavonoid metabolic pathways occurred in the JC pepper. Integrative analysis of metabolome and transcriptome suggested that four structural genes, 4CL7, 4CL6, CHS, and COMT, were responsible for the higher accumulation of metabolites relevant to capsaicin and flavonoids. Through weighted gene co-expression network analyses, modules related to flavonoid biosynthesis and potential regulators for candidate genes were identified. The promoter analysis of four candidate genes showed they contained several cis-elements that were bonded to MYB, bZIP, and WRKY transcription factors. Further RT-qPCR examination verified three transcription factors, MYB, bZIP53, and WRKY25, that exhibited increased expression in the red ripening stage of the JC pepper compared to YB, which potentially regulated their expression. Altogether, our findings provide comprehensive understanding and valuable information for pepper breeding programs in the future.
Di- and Triselenoesters—Promising Drug Candidates for the Future Therapy of Triple-Negative Breast Cancer
Authors: Radomska Dominika , Czarnomysy Robert , Szymanowska Anna , Radomski Dominik , Chalecka Magda , Surazynski Arkadiusz , Domínguez-Álvarez Enrique , Bielawska Anna , Bielawski Krzysztof
Published: July 2024

Breast cancer is a major malignancy among women, characterized by a high mortality rate. The available literature evidence indicates that selenium, as a trace element, has chemopreventive properties against many types of cancer; as such, compounds containing it in their structure may potentially exhibit anticancer activity. Accordingly, we have undertaken a study to evaluate the effects of novel selenoesters (EDAG-1, -7, -8, -10) on MCF-7 and MDA-MB-231 breast cancer cells. Our analysis included investigations of cell proliferation and viability as well as cytometric determinations of apoptosis/autophagy induction, changes in mitochondrial membrane polarity (ΔΨm), caspase 3/7, 8, and 9 activities, and Bax, Bcl-2, p53, Akt, AMPK, and LC3A/B proteins. The obtained data revealed that the tested derivatives are highly cytotoxic and inhibit cell proliferation even at nanomolar doses (0.41–0.79 µM). Importantly, their strong proapoptotic properties (↑ caspase 3/7) are attributable to the effects on both the extrinsic (↑ caspase 8) and intrinsic (↓ ΔΨm and Bcl-2, ↑ Bax, p53, and caspase 9) pathways of apoptosis. Moreover, the tested compounds are autophagy activators (↓ Akt, ↑ autophagosomes and autolysosomes, AMPK, LC3A/B). In summary, the potent anticancer activity suggests that the tested compounds may be promising drug candidates for future breast cancer therapy.
Increased Permeability of the Blood–Brain Barrier in a Diabetic Mouse Model (Leprdb/db Mice)
Authors: Alshammari Musaad A. , Alshehri Abdulaziz O. , Alqahtani Faleh , Khan Mohammad R. , Bakhrebah Muhammed A. , Alasmari Fawaz , Alshammari Tahani K. , Alsharari Shakir D.
Published: July 2024

Type 2 Diabetes Mellitus (T2DM) is linked to multiple complications, including cognitive impairment, and the prevalence of memory-related neurodegenerative diseases is higher in T2DM patients. One possible theory is the alteration of the microvascular and macrovascular environment of the blood–brain barrier (BBB). In this study, we employed different approaches, including RT-PCR, functional pharmacokinetic studies using sodium fluorescein (NaFL), and confocal microscopy, to characterize the functional and molecular integrity of the BBB in a T2DM animal model, leptin receptor-deficient mutant mice (Leprdb/db mice). As a result, VCAM-1, ICAM-1, MMP-9, and S100b (BBB-related markers) dysregulation was observed in the Leprdb/db animal model compared to littermate wild-type mice. The brain concentration of sodium fluorescein (NaFL) increased significantly in Leprdb/db untreated mice compared to insulin-treated mice. Therefore, the permeability of NaFL was higher in Leprdb/db control mice than in all remaining groups. Identifying the factors that increase the BBB in Leprdb/db mice will provide a better understanding of the BBB microvasculature and present previously undescribed findings of T2DM-related brain illnesses, filling knowledge gaps in this emerging field of research.
Synergic Effect of the Antimicrobial Peptide ToAP2 and Fluconazole on Candida albicans Biofilms
Authors: do Nascimento Dias Jhones , Hurtado Erazo Fabián Andrés , Bessa Lucinda J. , Eaton Peter , Leite José Roberto de Souza de Almeida , Paes Hugo Costa , Nicola André Moraes , Silva-Pereira Ildinete , Albuquerque Patrícia
Published: July 2024

Candida albicans is one of the agents of invasive candidiasis, a life-threatening disease strongly associated with hospitalization, particularly among patients in intensive care units with central venous catheters. This study aimed to evaluate the synergistic activity of the antifungal peptide ToAP2 combined with fluconazole against C. albicans biofilms grown on various materials. We tested combinations of different concentrations of the peptide ToAP2 with fluconazole on C. albicans biofilms. These biofilms were generated on 96-well plates, intravenous catheters, and infusion tubes in RPMI medium at two maturation stages. Scanning electron microscopy and atomic force microscopy were employed to assess the biofilm structure. We also evaluated the expression of genes previously proven to be involved in C. albicans biofilm formation in planktonic and biofilm cells after treatment with the peptide ToAP2 using qPCR. ToAP2 demonstrated a synergistic effect with fluconazole at concentrations up to 25 µM during both the early and mature stages of biofilm formation in 96-well plates and on medical devices. Combinations of 50, 25, and 12.5 µM of ToAP2 with 52 µM of fluconazole significantly reduced the biofilm viability compared to individual treatments and untreated controls. These results were supported by substantial structural changes in the biofilms observed through both scanning and atomic force microscopy. The gene expression analysis of C. albicans cells treated with 25 µM of ToAP2 revealed a decrease in the expression of genes associated with membrane synthesis, along with an increase in the expression of genes involved in efflux pumps, adhesins, and filamentation. Our results highlight the efficacy of the combined ToAP2 and fluconazole treatment against C. albicans biofilms. This combination not only shows therapeutic potential but also suggests its utility in developing preventive biofilm tools for intravenous catheters.
Stress-Related Chronic Fatigue Syndrome: A Case Report with a Positive Response to Alpha-Methyl-P-Tyrosine (AMPT) Treatment
Authors: Ljungström Maria , Oltra Elisa , Pardo Marta
Published: July 2024

Chronic fatigue syndrome (CFS) is a heterogeneous disorder with a genetically associated vulnerability of the catecholamine metabolism (e.g., catechol O-methyltransferase polymorphisms), in which environmental factors have an important impact. Alpha-methyl-p-tyrosine (AMPT; also referred to as metyrosine) is an approved medication for the treatment of pheochromocytoma. As a tyrosine hydroxylase inhibitor, AMPT may be a potential candidate for the treatment of diseases involving catecholamine alterations. However, only small-scale clinical trials have tested AMPT repurposing in a few other illnesses. The current case report compiles genetic and longitudinal biochemical data for over a year of follow-up of a male patient sequentially diagnosed with sustained overstress, neurasthenia, CFS (diagnosed in 2012 as per the Center for Disease Control (CDC/Fukuda)), and postural orthostatic tachycardia syndrome (POTS) over a 10-year period and reports the patient’s symptom improvement in response to low–medium doses of AMPT. This case was recognized as a stress-related CFS case. Data are reported from medical records provided by the patient to allow a detailed response to treatment targeting the hyperadrenergic state presented by the patient. We highlight the lack of a positive response to classical approaches to treating CFS, reflecting the limitations of CFS diagnosis and available treatments to alleviate patients’ symptoms. The current pathomechanism hypothesis emphasizes monoamine alterations (hyperadrenergic state) in the DA/adrenergic system and a dysfunctional autonomic nervous system resulting from sympathetic overactivity. The response of the patient to AMPT treatment highlights the relevance of pacing with regard to stressful situations and increased activity. Importantly, the results do not indicate causality between AMPT and its action on the monoamine system, and future studies should evaluate the implications of other targets.
Cellular and Molecular Biology of Mitochondria in Chronic Obstructive Pulmonary Disease
Authors: Li Chin-Ling , Liu Shih-Feng
Published: July 2024

Chronic obstructive pulmonary disease (COPD) is a progressive respiratory disorder characterized by enduring airflow limitation and chronic inflammation. Growing evidence highlights mitochondrial dysfunction as a critical factor in COPD development and progression. This review explores the cellular and molecular biology of mitochondria in COPD, focusing on structural and functional changes, including alterations in mitochondrial shape, behavior, and respiratory chain complexes. We discuss the impact on cellular signaling pathways, apoptosis, and cellular aging. Therapeutic strategies targeting mitochondrial dysfunction, such as antioxidants and mitochondrial biogenesis inducers, are examined for their potential to manage COPD. Additionally, we consider the role of mitochondrial biomarkers in diagnosis, evaluating disease progression, and monitoring treatment efficacy. Understanding the interplay between mitochondrial biology and COPD is crucial for developing targeted therapies to slow disease progression and improve patient outcomes. Despite advances, further research is needed to fully elucidate mitochondrial dysfunction mechanisms, discover new biomarkers, and develop targeted therapies, aiming for comprehensive disease management that preserves lung function and enhances the quality of life for COPD patients.
De Novo DNM1L Mutation in a Patient with Encephalopathy, Cardiomyopathy and Fatal Non-Epileptic Paroxysmal Refractory Vomiting
Authors: Berti Beatrice , Verrigni Daniela , Nasca Alessia , Di Nottia Michela , Leone Daniela , Torraco Alessandra , Rizza Teresa , Bellacchio Emanuele , Legati Andrea , Palermo Concetta , Marchet Silvia , Lamperti Costanza , Novelli Antonio , Mercuri Eugenio Maria , Bertini Enrico Silvio , Pane Marika , Ghezzi Daniele , Carrozzo Rosalba
Published: July 2024

Mitochondrial fission and fusion are vital dynamic processes for mitochondrial quality control and for the maintenance of cellular respiration; they also play an important role in the formation and maintenance of cells with high energy demand including cardiomyocytes and neurons. The DNM1L (dynamin-1 like) gene encodes for the DRP1 protein, an evolutionary conserved member of the dynamin family that is responsible for the fission of mitochondria; it is ubiquitous but highly expressed in the developing neonatal heart. De novo heterozygous pathogenic variants in the DNM1L gene have been previously reported to be associated with neonatal or infantile-onset encephalopathy characterized by hypotonia, developmental delay and refractory epilepsy. However, cardiac involvement has been previously reported only in one case. Next-Generation Sequencing (NGS) was used to genetically assess a baby girl characterized by developmental delay with spastic–dystonic, tetraparesis and hypertrophic cardiomyopathy of the left ventricle. Histochemical analysis and spectrophotometric determination of electron transport chain were performed to characterize the muscle biopsy; moreover, the morphology of mitochondria and peroxisomes was evaluated in cultured fibroblasts as well. Herein, we expand the phenotype of DNM1L-related disorder, describing the case of a girl with a heterozygous mutation in DNM1L and affected by progressive infantile encephalopathy, with cardiomyopathy and fatal paroxysmal vomiting correlated with bulbar transitory abnormal T2 hyperintensities and diffusion-weighted imaging (DWI) restriction areas, but without epilepsy. In patients with DNM1L mutations, careful evaluation for cardiac involvement is recommended.
Exploring Mitochondrial Interactions with Pulsed Electromagnetic Fields: An Insightful Inquiry into Strategies for Addressing Neuroinflammation and Oxidative Stress in Diabetic Neuropathy
Authors: Chianese Diego , Bonora Massimo , Sambataro Maria , Sambato Luisa , Paola Luca Dalla , Tremoli Elena , Cappucci Ilenia Pia , Scatto Marco , Pinton Paolo , Picari Massimo , Ferroni Letizia , Zavan Barbara
Published: July 2024

Pulsed electromagnetic fields (PEMFs) are recognized for their potential in regenerative medicine, offering a non-invasive avenue for tissue rejuvenation. While prior research has mainly focused on their effects on bone and dermo-epidermal tissues, the impact of PEMFs on nervous tissue, particularly in the context of neuropathy associated with the diabetic foot, remains relatively unexplored. Addressing this gap, our preliminary in vitro study investigates the effects of complex magnetic fields (CMFs) on glial-like cells derived from mesenchymal cell differentiation, serving as a model for neuropathy of the diabetic foot. Through assessments of cellular proliferation, hemocompatibility, mutagenicity, and mitochondrial membrane potential, we have established the safety profile of the system. Furthermore, the analysis of microRNAs (miRNAs) suggests that CMFs may exert beneficial effects on cell cycle regulation, as evidenced by the upregulation of the miRNAs within the 121, 127, and 142 families, which are known to be associated with mitochondrial function and cell cycle control. This exploration holds promise for potential applications in mitigating neuropathic complications in diabetic foot conditions.
Real-Time Precision in 3D Concrete Printing: Controlling Layer Morphology via Machine Vision and Learning Algorithms
Authors: Silva João M. , Wagner Gabriel , Silva Rafael , Morais António , Ribeiro João , Mould Sacha , Figueiredo Bruno , Nóbrega João M. , Cruz Paulo J. S.
Published: July 2024

3D concrete printing (3DCP) requires precise adjustments to parameters to ensure accurate and high-quality prints. However, despite technological advancements, manual intervention still plays a prominent role in this process, leading to errors and inconsistencies in the final printed part. To address this issue, machine learning vision models have been developed and utilized to analyze captured images and videos of the printing process, detecting defects and deviations. The data collected enable automatic adjustments to print settings, improving quality without the need for human intervention. This work first examines various techniques for real-time and offline corrections. It then introduces a specialized computer vision setup designed for real-time control in robotic 3DCP. Our main focus is on a specific aspect of machine learning (ML) within this system, called speed control, which regulates layer width by adjusting the robot motion speed or material flow rate. The proposed framework consists of three main elements: (1) a data acquisition and processing pipeline for extracting printing parameters and constructing a synthetic training dataset, (2) a real-time ML model for parameter optimization, and (3) a depth camera installed on a customized 3D-printed rotary mechanism for close-range monitoring of the printed layer.
Regeneration of Osteochondral Lesion of the Talus with Retrograde Drilling Technique: An In Vitro Pilot Study
Authors: Veronesi Francesca , Maglio Melania , Brogini Silvia , Mazzotti Antonio , Artioli Elena , Zielli Simone Ottavio , Faldini Cesare , Giavaresi Gianluca
Published: July 2024

Background: Retrograde Drilling (RD) is a surgical technique employed for osteochondral lesions of the talus (OCLTs) to reach the subchondral bone lesion from behind, thus preserving cartilage integrity. The aim of the present pilot study was to set up an in vitro model of OCLTs to evaluate the regenerative potential of biological approaches that could be associated with the RD technique. Methods: For this purpose, an OCLT was created in human osteochondral specimens, to try to mimic the RD technique, and to compare the regenerative potential of two biological treatments. For this purpose, three groups of treatments were performed in vitro: (1) no treatment (empty defect); (2) autologous bone graft (ABG); (3) hyaluronic membrane enriched with autologous bone marrow cells. Tissue viability; production of Collagen I and II, Vascular Endothelial Growth Factor, and Aggrecan; and histological and microCT evaluations were performed after 30 days of culture in normal culture conditions. Results: It was observed that Group 3 showed the highest viability, and Group 2 showed the highest protein production. From a histological and microtomographic point of view, it was possible to appreciate the structure of the morcellized bone with which the defect of Group 2 was filled, while it was not yet possible to observe the deposition of mineralized tissue in Group 3. Conclusions: To conclude, this pilot study shows the feasibility of an alternative in vitro model to evaluate and compare the regenerative potential of two biological scaffolds, trying to mimic the RD technique as much as possible. The tissues remained vital for up to 4 weeks and both ABG and hyaluronic acid-based scaffolds stimulated the release of proteins linked to regenerative processes in comparison to the empty defect group.
Risk Factors for Rectal Cancer Recurrence after Local Excision of T1 Lesions from a Decade-Long Multicenter Retrospective Study
Authors: Rudnicki Yaron , Goldberg Nitzan , Horesh Nir , Harbi Assaf , Lubianiker Barak , Green Eraan , Raveh Guy , Slavin Moran , Segev Lior , Gilshtein Haim , Barenboim Alexander , Wasserberg Nir , Khaikin Marat , Tulchinsky Hagit , Issa Nidal , Duek Daniel , Avital Shmuel , White Ian
Published: July 2024

Background: Local surgical excision of T1 rectal adenocarcinoma is a well-established approach. Yet, there are still open questions regarding the recurrence rates and its risk factors. Methods: A retrospective multicenter study including all patients who underwent local excision of early rectal cancer with an open or MIS approach and had a T1 lesion from 2010 to 2020 in six academic centers. Data included demographics, preoperative studies, surgical findings, postoperative outcomes, and local and systemic recurrence. A univariable and multivariable logistic regression analysis was performed to identify risk factors for recurrence. Results: Overall, 274 patients underwent local excision of rectal lesions. Of them, 97 (35.4%) patients with a T1 lesion were included in the cohort. The mean age was 69 ± 10.5 years, and 42 (43.3%) were female. The mean distance of the lesions from the anal verge was 7.8 ± 3.2 cm, and the average tumor size was 2.7 ± 1.6 cm. Eighty-two patients (85%) had a full-thickness resection. Eight patients (8%) had postoperative complications. Kikuchi classification of submucosal (SM) involvement was reported in 29 (30%) patients. Twelve patients had SM1, two SM2, and fifteen SM3. Following pathology, 24 patients (24.7%) returned for additional surgery or treatment. The overall recurrence rate was 14.4% (14 patients), with 11 patients having a local recurrence and 6 having a systemic metastatic recurrence, 3 of which had both. The mean time for recurrence was 2.78 ± 2.8 years and the overall mortality rate was 11%. On univariable and multivariable logistic regression analysis of recurrence vs. non-recurrence groups, the strongest and most significant association and possible risk factors for recurrence were larger lesions (4.3 vs. 2.5 cm, p < 0.001) with an OR of 6.67 (CI—1.82–24.36), especially for tumors larger than 3.5 cm, mucinous histology (14.3% vs. 1.2%, p = 0.004, OR of 14.02, CI—1.13–173.85), and involved margins (41.7% vs. 16.2%, p = 0.003, OR of 9.59, CI—2.14–43.07). The open transanal excision (TAE) approach was also identified as a possible significant risk factor in univariant analysis, while SM3 level penetration showed only a trend. Conclusion: Surgical local excision of T1 rectal malignancy is a safe and viable option. Still, one in four patients received additional treatment. There is an almost 15% chance for recurrence, especially in large tumors, mucinous histology, or involved margin cases. These high-risk patients might warrant additional intervention and stricter surveillance protocols.
Primary Open-Angle Glaucoma Is Associated with Short-Term Memory Decline and Dementia in Individuals of African Ancestry
Authors: Sapir Tzuriel , Augello Patrick , Lee Roy , McCoskey Makayla , Salowe Rebecca , Addis Victoria , Sankar Prithvi , Ying Gui-Shuang , O’Brien Joan M.
Published: July 2024

Background: Over the last decade, studies have suggested that primary open-angle glaucoma (POAG) may be associated with cognitive impairment and dementia, as both pathologies are age-related neurodegenerative processes. It remains unclear to what extent neurodegeneration in POAG extends to other neurological functions beyond vision, such as cognition. This follow-up study examined the potential association between POAG and cognitive decline in an African ancestry population. Methods: The Telephone-Montreal Cognitive Assessment (T-MoCA) was administered to POAG cases and controls previously enrolled in the Primary Open-Angle African American Glaucoma Genetics (POAAGG) study. Cases were assessed for retinal nerve fiber layer (RNFL) thickness and for the presence of dementia via chart review. Comparisons between POAG cases and controls were performed using two-sample t-tests for the T-MoCA total score and five subsection scores, and using chi-squared tests for incidence of dementia. Current scores were compared to scores from this same cohort from 7 years prior. Results: The T-MoCA was administered to 13 cases and 20 controls. The mean ± standard deviation (SD) T-MoCA total score was 15.5 ± 4.0 in cases and 16.7 ± 3.5 in controls (p = 0.36). However, there was a borderline significant difference in the delayed recall sub-score (2.3 ± 1.6 for cases vs. 3.4 ± 1.5 for controls, p = 0.052) and a significant difference in its sub-domain, the memory index score (MIS, 9.1 ± 4.3 for cases vs. 12.1 ± 3.0 for controls, p = 0.02). There were no significant differences between cases and controls for the remaining subsections. During 7 years of follow-up, a higher incidence of dementia was noted in POAG cases (7.1% for cases vs. 0% for controls, p = 0.058). Over 7 years, there was no significant deterioration in the cognitive performance of cases versus controls, and no association was seen between RNFL thinning and cognitive impairment. Conclusions: In this small-sample follow-up study of African ancestry individuals, POAG cases demonstrated worse short-term memory and higher incidence of dementia compared to controls. Future larger studies are needed to further investigate the presence and impact of neurodegeneration in POAG.
Using Deep Learning to Distinguish Highly Malignant Uveal Melanoma from Benign Choroidal Nevi
Authors: Hoffmann Laura , Runkel Constance B. , Künzel Steffen , Kabiri Payam , Rübsam Anne , Bonaventura Theresa , Marquardt Philipp , Haas Valentin , Biniaminov Nathalie , Biniaminov Sergey , Joussen Antonia M. , Zeitz Oliver
Published: July 2024

Background: This study aimed to evaluate the potential of human–machine interaction (HMI) in a deep learning software for discerning the malignancy of choroidal melanocytic lesions based on fundus photographs. Methods: The study enrolled individuals diagnosed with a choroidal melanocytic lesion at a tertiary clinic between 2011 and 2023, resulting in a cohort of 762 eligible cases. A deep learning-based assistant integrated into the software underwent training using a dataset comprising 762 color fundus photographs (CFPs) of choroidal lesions captured by various fundus cameras. The dataset was categorized into benign nevi, untreated choroidal melanomas, and irradiated choroidal melanomas. The reference standard for evaluation was established by retinal specialists using multimodal imaging. Trinary and binary models were trained, and their classification performance was evaluated on a test set consisting of 100 independent images. The discriminative performance of deep learning models was evaluated based on accuracy, recall, and specificity. Results: The final accuracy rates on the independent test set for multi-class and binary (benign vs. malignant) classification were 84.8% and 90.9%, respectively. Recall and specificity ranged from 0.85 to 0.90 and 0.91 to 0.92, respectively. The mean area under the curve (AUC) values were 0.96 and 0.99, respectively. Optimal discriminative performance was observed in binary classification with the incorporation of a single imaging modality, achieving an accuracy of 95.8%. Conclusions: The deep learning models demonstrated commendable performance in distinguishing the malignancy of choroidal lesions. The software exhibits promise for resource-efficient and cost-effective pre-stratification.
Advances in COVID-19 and Cancer Research
Authors: Franza Laura , Cianci Rossella
Published: July 2024

The global health crisis caused by COVID-19 has radically changed the management of several diseases [...]
Crohn’s Disease: Radiological Answers to Clinical Questions and Review of the Literature
Authors: Minordi Laura Maria , D’Angelo Francesca Bice , Privitera Giuseppe , Papa Alfredo , Larosa Luigi , Laterza Lucrezia , Scaldaferri Franco , Barbaro Brunella , Carbone Luigi , Pugliese Daniela
Published: July 2024

Background: Crohn’s disease (CD) is a chronic, progressive inflammatory condition, involving primarily the bowel, characterized by a typical remitting–relapsing pattern. Despite endoscopy representing the reference standard for the diagnosis and assessment of disease activity, radiological imaging has a key role, providing information about mural and extra-visceral involvement. Methods: Computed Tomography and Magnetic Resonance Imaging are the most frequently used radiological techniques in clinical practice for both the diagnosis and staging of CD involving the small bowel in non-urgent settings. The contribution of imaging in the management of CD is reported on by answering the following practical questions: (1) What is the best technique for the assessment of small bowel CD? (2) Is imaging a good option to assess colonic disease? (3) Which disease pattern is present: inflammatory, fibrotic or fistulizing? (4) Is it possible to identify the presence of strictures and to discriminate inflammatory from fibrotic ones? (5) How does imaging help in defining disease extension and localization? (6) Can imaging assess disease activity? (7) Is it possible to evaluate post-operative recurrence? Results: Imaging is suitable for assessing disease activity, extension and characterizing disease patterns. CT and MRI can both answer the abovementioned questions, but MRI has a greater sensitivity and specificity for assessing disease activity and does not use ionizing radiation. Conclusions: Radiologists are essential healthcare professionals to be involved in multidisciplinary teams for the management of CD patients to obtain the necessary answers for clinically relevant questions.
Complications of Multisystem Inflammatory Syndrome Associated with SARS-CoV-2 Infection—Many Facets of One Disease—A Literature Review Based on a Case Report
Authors: Stasiak Aleksandra , Kędziora Piotr , Smolewska Elżbieta
Published: July 2024

Multisystem inflammatory syndrome in children (MIS-C) is a disease that made its mark in the early days of the COVID-19 pandemic due to the diverse course and symptoms affecting multiple body systems. It is a condition that develops in pediatric patients about 2–6 weeks after contact with a person infected with the SARS-CoV-2 virus. In many instances, MIS-C has caused multiple organ failure, with particularly severe complications involving the cardiovascular system and manifesting as hypotension, various cardiac arrhythmias, myocarditis or coronary artery lesions resembling those seen in Kawasaki disease. Currently, the incidence of MIS-C is about 1–3 per 1000 children, with a decreasing trend in recent years due to the introduction of immunization against the SARS-CoV-2 virus for children as young as 6 months. In our paper, we present the case of a patient with a severe course of MIS-C with numerous cardiovascular and neurological complications, in whom the symptoms of the disease were managed by administering biological treatment. We also present a review of the literature on the subject, which shows how many different facets this disease can have and that physicians still need to remain alert, as there are cases of severe MIS-C, especially in unvaccinated patients.
The Clinical Impact of Different Types of Preoperative Biliary Intervention on Postoperative Biliary Tract Infection of Patients Undergoing Pancreaticoduodenectomy
Authors: Wu Min-Jung , Chan Yung-Yuan , Chen Ming-Yang , Hung Yu-Liang , Kou Hao-Wei , Tsai Chun-Yi , Hsu Jun-Te , Yeh Ta-Sen , Hwang Tsann-Long , Jan Yi-Yin , Wu Chi-Huan , Liu Nai-Jen , Wang Shang-Yu , Yeh Chun-Nan
Published: July 2024

Background: For patients with obstructive jaundice and who are indicated for pancreaticoduodenectomy (PD) or biliary intervention, either endoscopic retrograde cholangiopancreatography (ERCP) or percutaneous transhepatic cholangiography and drainage (PTCD) may be indicated preoperatively. However, the possibility of procedure-related postoperative biliary tract infection (BTI) should be a concern. We tried to evaluate the impact of ERCP and PTCD on postoperative BTI. Methods: Patients diagnosed from June 2013 to March 2022 with periampullary lesions and with PD indicated were enrolled in this cohort. Patients without intraoperative bile culture and non-neoplastic lesions were excluded. Clinical information, including demographic and laboratory data, pathologic diagnosis, results of microbiologic tests, and relevant infectious outcomes, was extracted from medical records for analysis. Results: One-hundred-and-sixty-four patients from the cohort (164/689) underwent preoperative biliary intervention, either ERCP (n = 125) or PTCD (n = 39). The positive yield of intraoperative biliary culture was significantly higher in patients who underwent ERCP than in PTCD (90.4% vs. 41.0%, p < 0.001). Although there was no significance, a trend of higher postoperative BTI (13.8% vs. 2.7%) and BTI-related septic shock (5 vs. 0, 4.0% vs. 0%) in the ERCP group was noticed. While the risk factors for postoperative BTI have not been confirmed, a trend suggesting a higher incidence of BTI associated with ERCP procedures was observed, with a borderline p-value (p = 0.05, regarding ERCP biopsy). Conclusions: ERCP in patients undergoing PD increases the positive yield of intraoperative biliary culture. PTCD may be the favorable option if preoperative biliary intervention is indicated.
Impact of Frailty on Functional Improvement Following Traumatic Spinal Cord Injury: A Japanese Single-Center Experience
Authors: Konomi Tsunehiko , Yoshikawa Minako , Kajikawa Keita , Kitagawa Takahiro , Kobayashi Yoshiomi , Furukawa Mitsuru , Fujiyoshi Kanehiro , Yato Yoshiyuki
Published: July 2024

Study Design: This is a retrospective case series study. Objective: The aim of this study was to investigate whether frailty contributes to functional recovery in individuals with spinal cord injury (SCI). Methods: A total of 121 patients with SCI (106 cervical SCI, 15 thoracic SCI) discharged from our center over the past three years were studied. Moreover, 11-factor modified frailty index (mFI) scores, the length of hospital stays, the rate of returning home, and improvement in Spinal Cord Independence Measure (SCIM) scores were assessed retrospectively. Results: The average age at the time of injury for all 121 cases was 59.6 years. Based on pre-injury assessments, 24 cases were categorized as the Frail group, and 97 cases were categorized as the Robust group. The Frail group had SCIM improvement rates of 16.7% and a home discharge rate of 45.8%. In contrast, the Robust group had SCIM improvement rates of 33.5% and a home discharge rate of 68.0%, with statistically significant differences between the two groups. A significant negative correlation was observed between mFI scores and SCIM improvement rates (R = −0.231, p = 0.014). Conclusions: This study suggests that individuals with pre-existing frailty before SCI experience poorer SCIM improvement rates and face challenges in returning home.
A Proposal for a Carbon Fibre-Manufacturing Life-Cycle Inventory: A Case Study from the Competitive Sailing Boat Industry
Authors: Jacquet Lucas , le Duigou Antoine , Kerbrat Olivier
Published: July 2024

The competitive sailing boat industry uses carbon fibre for high-performance purposes. Nevertheless, this material is known to cause environmental issues during its manufacturing. We can currently observe, based on the literature, difficulty integrating a reliable, justified, and transparent inventory of carbon-fibre production for LCA applications of high-performance composite materials. The current study aims to gain a better understanding of carbon fibre’s environmental impacts by suggesting a justified, reliable, and transparent inventory, based on the life-cycle assessment methodology. It also aims at providing a LCA of high-performance composites. An EcoInvent flows inventory is suggested, based on the literature presenting primary inventories. It is then discussed in terms of data quality, flows under study, and indicators calculated. Eventually, the inventory is used to assess the environmental impact of carbon fibre-reinforced composites applied to an industrial example representative of the competitive sailing boat industry: a hydrofoil mould. Regarding results on carbon fibres’ scale and impacts, indicators commonly highlighted by the literature, were calculated in this study (GWP = 72 kgCO2eq and CED = 1176 MJ), as well as other indicators. These indicators are two to five times higher than the inventories suggested in the literature, due to high heat-production value, production scales, or the quality of the fibre under study. The composite scale results show a major contribution from carbon fibre compared to other flows under study, highlighting the need to suggest a reliable inventory of carbon-fibre production.
Probiotic Yeasts: A Developing Reality?
Authors: Tullio Vivian
Published: July 2024

Yeasts are gaining increasing attention for their potential health benefits as probiotics in recent years. Researchers are actively searching for new yeast strains with probiotic properties (i.e, Debaryomyces hansenii; Kluyveromyces marxianus; Yarrowia lipolytica; Pichia hudriavzevii; and Torulaspora delbrueckii) from various sources, including traditional fermented foods, the human gut, and the environment. This exploration is expanding the pool of potential probiotic yeasts beyond the well-studied Saccharomyces boulardii. Research suggests that specific yeast strains possess properties that could be beneficial for managing conditions like inflammatory bowel disease, irritable bowel syndrome, skin disorders, and allergies. Additionally, probiotic yeasts may compete with pathogenic bacteria for adhesion sites and nutrients, thereby inhibiting their growth and colonization. They might also produce antimicrobial compounds that directly eliminate harmful bacteria. To achieve these goals, the approach that uses probiotics for human health is changing. Next-generation yeast probiotics are emerging as a powerful new approach in the field of live biotherapeutics. By using genetic engineering, scientists are able to equip these tools with specialized capabilities. However, most research on these probiotic yeasts is still in its early stages, and more clinical trials are needed to confirm their efficacy and safety for various health conditions. This review could provide a brief overview of the situation in this field.