bluuebunny/crossref_metadata_2025 huggingface dataset:
A Review—Durability, Mechanical and Hygrothermal Behavior of Building Materials Incorporating Biomass
Authors: Affan Houssam , El Haddaji Badreddine , Ajouguim Soukaina , Khadraoui Fouzia
Published: June 2024

The growing importance of environmental efficiency in reducing carbon emissions has prompted scientists around the world to intensify their efforts to prevent the destructive effects of a changing climate and a warming planet. Global carbon emissions rose by more than 40% in 2021, leading to significant variations in the planet’s weather patterns. Nevertheless, a significant proportion of natural resources continue to be exploited. To prepare for this challenge, it is essential to implement a sustainable approach in the construction industry. Biobased materials are made primarily from renewable raw materials like hemp, straw, miscanthus, and jute. These new materials provide excellent thermal and acoustic performance and make optimum use of local natural resources such as agricultural waste. Nowadays, cement is one of the most important construction materials. In an attempt to meet this exciting challenge, biobased materials with low-carbon binders are one of the proposed solutions to create a more insulating and less polluting material. The aim of this review is to investigate and to analyze the impact of the incorporation of different types of biobased materials on the mechanical, thermal, and hygric performance of a mix using different types of binder.
Simultaneous Determination of 17 Phenolic Compounds in Surface Water and Wastewater Matrices Using an HPLC-DAD Method
Authors: Paun Iuliana , Pascu Luoana Florentina , Iancu Vasile Ion , Pirvu Florinela , Galaon Toma , Chiriac Florentina Laura
Published: June 2024

A high-performance liquid chromatography with diode array detector (HPLC-DAD) analytical method was developed for the simultaneous detection of 17 phenolic compounds, including phenols, chlorophenols, alkylphenols, and nitrophenols, in two types of water matrices: wastewater and surface water. Prior to HPLC-DAD determination, a solid-phase extraction (SPE) procedure was optimized. The proposed method uses multiwavelength analysis, with the optimum detection wavelengths selected as 268 nm, 280 nm, 386 nm, 304 nm, and 316 nm. The highest resolution was achieved using a chromatographic column, Eclipse XDB-C18 (150 × 4.6 mm, 5 μm), which was kept at 20 °C. The mobile phase consisted of a gradient elution program, with mobile phase A being a 0.1% H3PO4 aqueous solution and mobile phase B being acetonitrile. The flow rate was set at 0.6 mL/min. The 17 target phenolic compounds were fully separated in less than 27 min. All compounds showed good linear regression, with correlation coefficients higher than 0.999. The method’s quantitation limits ranged from 4.38 to 89.7 ng/L for surface water and 7.83 to 167 ng/L for wastewater. The recovery rates were in the range of 86.2–95.1% for surface water and 79.1–86.3% for wastewater. The SPE-HPLC-DAD method was proven to be fast, sensitive, accurate, and reproducible. The developed method was successfully applied for the analysis of the 17 phenolic compounds in real surface water and wastewater samples, with phenol, 2,4-DNP, and 2,4-DNP being determined at levels greater than the method’s limits of quantitation (LOQs). The proposed analytical method represents an original technical resource for the simultaneous determination of 17 phenolic compounds in environmental water matrices.
DCP-Net: An Efficient Image Segmentation Model for Forest Wildfires
Authors: Qiao Lei , Yuan Wei , Tang Liu
Published: June 2024

Wildfires usually lead to a large amount of property damage and threaten life safety. Image recognition for fire detection is now an important tool for intelligent fire protection, and the advancement of deep learning technologies has enabled an increasing number of cameras to possess functionalities for fire detection and automatic alarm triggering. To address the inaccuracies in extracting texture and positional information during intelligent fire recognition, we have developed a novel network called DCP-Net based on UNet, which excels at capturing flame features across multiple scales. We conducted experiments using the Corsican Fire Dataset produced by the “Environmental Science UMR CNRS 6134 SPE” laboratory at the University of Corsica and the BoWFire Dataset by Chino et al. Our algorithm was compared with networks such as SegNet, UNet, UNet++, and PSPNet, demonstrating superior performance across three metrics: mIoU, F1-score, and OA. Our proposed deep learning model achieves the best mIoU (78.9%), F1-score (76.1%), and OA (96.7%). These results underscore the robustness of our algorithm, which accurately identifies complex flames, thereby making a significant contribution to intelligent fire recognition. Therefore, the proposed DCP-Net model offers a viable solution to the challenges of wildfire monitoring using cameras, with hardware and software requirements typical of deep learning setups.
Fast-Curing 3-Layer Particleboards with Lignosulfonate and pMDI Adhesives
Authors: Gonçalves Sofia , Paiva Nádia T. , Martins Jorge , Carvalho Luísa H. , Magalhães Fernão D.
Published: June 2024

Currently, the industrial success of bio-based adhesives remains limited, despite the growing interest in these compounds. One example is the use of lignosulfonates (LS), a byproduct from the pulp and paper industry, which requires long pressing times to ensure proper performance for wood-based panel production. This study successfully manufactured particleboards using a low press factor of 7.5 s/mm, commonly used for conventional urea-formaldehyde resins on a lab scale. To the best of our knowledge, lignin-based particleboards have never been reported using such low press factors. Thus, 3-layer boards were manufactured in which the core layer was bonded with polymeric isocyanate (pMDI), and the surface layers were bonded with LS. Propylene carbonate (PC) was used as a solvent for pMDI to improve adhesive distribution. The optimum amounts of adhesive were determined using response surface methodology: 1.3% pMDI with 2.2% PC in the core layer and 15% LS in the surface layers. These boards obeyed the requirements of standard EN 312 for general-purpose boards for use in dry conditions (type P1). Their formaldehyde content, determined through the perforator method, was equal to that of the wood mix at the maximum value set by IKEA for class E0.5.
The Relationship between Trait-Based Functional Niche Hypervolume and Community Phylogenetic Structures of Typical Forests across Different Climatic Zones in China
Authors: Huang Jihong , Yu Ruoyun , Ding Yi , Xu Yue , Yao Jie , Zang Runguo
Published: June 2024

Functional traits are pivotal for understanding the functional niche within plant communities. Yet, the relationship between the functional niches of typical forest plant communities across different climatic zones, as defined by functional traits, and their association with community and phylogenetic structures remains elusive. In this study, we examined 215 woody species, incorporating 11 functional traits spanning leaf economy, mechanical support, and reproductive phenology, gathered from forests in four climatic zones from tropical, subtropical, warm-temperate to cold-temperate zones in China and supplemented by the literature. We quantified the functional niche hypervolume (FNH), reflecting the multidimensional functional niche variability. We then probed into the correlation between the FNH and community and phylogenetic structures of forests. Our findings reveal that species richness significantly influences the geographic variance of functional niche space in forest vegetation across different climatic zones. Specifically, a community’s species richness correlates positively with the functional niche breadth occupied by the community species. The FNH of woody plants across diverse forest types shows significant associations with both the mean phylogenetic distance (MPD) and the mean nearest phylogenetic taxon distance (MNTD) of the communities. There is a progressive increase in tropical rainforest (TF), subtropical evergreen deciduous broad-leaved mixed forest (SF), and warm-temperate coniferous broad-leaved mixed forest (WF), followed by a decline in the cold-temperate coniferous forest (CF). This pattern suggests potential environmental filtering in CF, which may constrain the spatial extent of plant functional niches. Our research underscores the substantial variability in the FNH across China’s typical forest vegetation, highlighting the complex interplay between functional traits, community richness, and phylogenetic distance.
Discrepancies and Evaluation of Needle-Leaf and Tracheid Traits of Qinhai Spruce in Qilian Mountains, Northwest China
Authors: Zhao Hu , Xu Erwen , Lv Dong , Wang Yanxia , Zhao Xingpeng , Wei Na , Zhang Zhengzhong , Yuan Hao , Ma Xuee , Wu Xiurong , Liu Xiande
Published: June 2024

Phenotypic variation analysis and comprehensive evaluation are important tools for selecting provenances of forest trees, which, in turn, is crucial for genetic improvement of forest trees. The study analyzed eight needle-leaf and fourteen tracheid trait indexes of ten provenances of Qinghai spruce forest stands. Multiple comparisons, correlation analysis, cluster analysis, and principal component analysis were used to evaluate the differences in needle-leaf and tracheid traits among the different provenances. The study found significant differences in the needle-leaf and tracheid traits among the provenances. All traits included 50 pairs of positive and 26 pairs of negative correlations. The coefficient of variation for the tracheid trait (18.86%) was higher than that for the needle-leaf trait (17.20%). A comprehensive evaluation of needle-leaf and tracheid traits was conducted using principal component analysis. The study demonstrated that the degree of variation in needle-leaf and tracheid traits among the provenances of Qinghai spruce was extensive, with a multitude of sources of variation. Therefore, it is crucial to strengthen the selection of good provenances in the early seed selection process. The results of the comprehensive evaluation can be utilized as a foundation for the selection of good provenances for carbon sequestration and timber properties.
Forest Biological Disaster Control Behaviors of Forest Farmers and Their Spatial Heterogeneity in China
Authors: Cai Qi , Sun Bowen , Zhang Xufeng , Bo Wenjing , Wang Guangyu , Zhou Zefeng
Published: June 2024

With more and more loss caused by forest biological disasters (FBDs) in China, forest farmers, as one of the most important stakeholders, are participating in the control. In this study, the ordinary least squares model, simultaneous equation model, and propensity score matching method were used with the data from 818 surveys conducted in the typical FBD outbreak provinces, to reveal the FBD control behaviors of forest farmers and their differences between western and eastern regions of China. The results indicated the following. (1) Household factors: forest farmers could timely take control measures. An increase of 1 ha in the area of occurrence would increase the control measures by 3.26 ha. However, the control measures can only reduce 50% of the economic loss caused by FBDs and cannot effectively control the spread trend of FBDs. There are issues, including an insufficient and old labor force, insufficient technology support, and low consciousness of ecology protection. (2) External factors: forest farmers would increase control when the temperature rises, and reduce control when rainfall increases. After village committees unify organizing the control, their participation enthusiasm would increase, which would have a substitution relationship with the household investment. (3) Regional difference: the eastern region of China has higher figures than the western in terms of outbreak area, economic losses, control measures, and pesticide cost. If the western forest farmers have the control funds as the eastern forest farmers have, and the eastern forest farmers have the control intensity as the western forest farmers have, the overall FBD control effect would be better.
The Zootechnical Performance, Health State Modulation, Morphology, and Intestinal Microbiome of Nile Tilapia Juveniles Fed with a Functional Blend of Immunostimulants Associated with a Diet High in Soybean Meal
Authors: Sá Lúvia Souza de , Jerônimo Gabriela Tomas , Soligo Thiago , Yamashita Eduardo , Machado Fracalossi Débora , Martins Maurício Laterça , Mouriño José Luiz Pedreira
Published: June 2024

The aim of this study was to evaluate the effect of supplementing feed with a functional mixture of immunomodulators, including β-glucans, nucleotides, ascorbic acid, and alpha-tocopherol, associated with a diet with lower levels of animal protein (11.5%) and higher levels of soybean meal (43.5%), on the zootechnical performance, health, hematological and immunological parameters, intestinal morphology, centesimal composition, and intestinal microbiome of juvenile Nile tilapia (initial weight 1.88 g ± 0.25 g, mean ± standard deviation). Two isocaloric and isoproteic diets (35% crude protein) were formulated, one with the inclusion of the immunostimulant functional mixture (40 kg·t−1), composed of 150 mg·kg−1 of nucleotides, 1000 mg·kg−1 of β-glucans, 1000 mg·kg−1 of ascorbic acid (vitamin C), and 20 mg·kg−1 of alpha-tocopherol (vitamin E), and another without. The combined supplementation of nucleotides, β-glucans, ascorbic acid, and alpha-tocopherol resulted in a 59.95% increase in final weight, 64% weight gain, 66% daily gain, a 21.31% decrease in feed conversion rate, and double the retention of body protein. Supplementation also improved intestinal morphology and modulated the intestinal microbiome, increasing Chao-1 diversity. Transmission electron microscopy confirmed that fish fed with both diets exhibited intact intestinal mucosal membranes. Supplementation did not alter the hematological and immunological parameters, suggesting that there was no overstimulation of the fish’s immune system. This work allows us to evaluate the effect of reducing the use of animal protein in the diets of fish, along with the effects of nucleotides, β-glucans, ascorbic acid, and alpha-tocopherol. Together, these compounds can provide fish with the necessary tools to achieve optimal health and growth.
Acid–Heat-Induced Fabrication of Nisin-Loaded Egg White Protein Nanoparticles: Enhanced Structural and Antibacterial Stability
Authors: Rao Shengqi , Jia Caochen , Lu Xiangning , Yu Yisheng , Wang Zhirong , Yang Zhenquan
Published: June 2024

As a natural cationic peptide, Nisin is capable of widely inhibiting the growth of Gram-positive bacteria. However, it also has drawbacks such as its antimicrobial activity being susceptible to environmental factors. Nano-encapsulation can improve the defects of nisin in food applications. In this study, nisin-loaded egg white protein nanoparticles (AH-NEn) were prepared in fixed ultrasound-mediated under pH 3.0 and 90 °C. Compared with the controls, AH-NEn exhibited smaller particle size (112.5 ± 2.85 nm), smaller PDI (0.25 ± 0.01), larger Zeta potential (24 ± 1.18 mV), and higher encapsulation efficiency (91.82%) and loading capacity (45.91%). The turbidity and Fourier transform infrared spectroscopy (FTIR) results indicated that there are other non-covalent bonding interactions between the molecules of AH-NEn besides the electrostatic forces, which accounts for the fact that it is structurally more stable than the controls. In addition, by the results of fluorescence intensity, differential scanning calorimetry (DSC), and X-ray diffraction (XRD), it was shown that thermal induction could improve the solubility, heat resistance, and encapsulation of nisin in the samples. In terms of antimicrobial function, acid–heat induction did not recede the antimicrobial activity of nisin encapsulated in egg white protein (EWP). Compared with free nisin, the loss rate of bactericidal activity of AH-NEn was reduced by 75.0% and 14.0% following treatment with trypsin or a thermal treatment at 90 °C for 30 min, respectively.
Phytosterols: Physiological Functions and Potential Application
Authors: Shen Mingyue , Yuan Lanlan , Zhang Jian , Wang Xufeng , Zhang Mingyi , Li Haizhen , Jing Ying , Zeng Fengjiao , Xie Jianhua
Published: June 2024

Dietary intake of natural substances to regulate physiological functions is currently regarded as a potential way of promoting health. As one of the recommended dietary ingredients, phytosterols that are natural bioactive compounds distributed in plants have received increasing attention for their health effects. Phytosterols have attracted great attention from scientists because of many physiological functions, for example, cholesterol-lowering, anticancer, anti-inflammatory, and immunomodulatory effects. In addition, the physiological functions of phytosterols, the purification, structure analysis, synthesis, and food application of phytosterols have been widely studied. Nowadays, many bioactivities of phytosterols have been assessed in vivo and in vitro. However, the mechanisms of their pharmacological activities are not yet fully understood, and in-depth investigation of the relationship between structure and function is crucial. Therefore, a contemporaneous overview of the extraction, beneficial properties, and the mechanisms, as well as the current states of phytosterol application, in the food field of phytosterols is provided in this review.
Development of an Apple Snack Enriched with Probiotic Lacticaseibacillus rhamnosus: Evaluation of the Refractance Window Drying Process on Cell Viability
Authors: Nuñez Helena , Jaques Aldonza , Belmonte Karyn , Elitin Jamil , Valdenegro Mónika , Ramírez Cristian , Córdova Andrés
Published: June 2024

The objective of this study was to develop a dried apple snack enriched with probiotics, evaluate its viability using Refractance Window (RWTM) drying, and compare it with conventional hot air drying (CD) and freeze-drying (FD). Apple slices were impregnated with Lacticaseibacillus rhamnosus and dried at 45 °C using RWTM and CD and FD. Total polyphenol content (TPC), color (∆E*), texture, and viable cell count were measured, and samples were stored for 28 days at 4 °C. Vacuum impregnation allowed for a probiotic inoculation of 8.53 log CFU/gdb. Retention values of 6.30, 6.67, and 7.20 log CFU/gdb were observed for CD, RWTM, and FD, respectively; the population in CD, RWTM remained while FD showed a decrease of one order of magnitude during storage. Comparing RWTM with FD, ∆E* was not significantly different (p < 0.05) and RWTM presented lower hardness values and higher crispness than FD, but the RWTM-dried apple slices had the highest TPC retention (41.3%). Microstructural analysis showed that RWTM produced a smoother surface, facilitating uniform moisture diffusion and lower mass transfer resistance. The effective moisture diffusion coefficient was higher in RWTM than in CD, resulting in shorter drying times. As a consequence, RWTM produced dried apple snacks enriched with probiotics, with color and TPC retention comparable to FD.
The Covariety of Saturated Numerical Semigroups with Fixed Frobenius Number
Authors: Rosales José Carlos , Moreno-Frías María Ángeles
Published: June 2024

In this work, we show that if F is a positive integer, then Sat(F)={S∣S is a saturated numerical semigroup with Frobenius number F} is a covariety. As a consequence, we present two algorithms: one that computes Sat(F), and another which computes all the elements of Sat(F) with a fixed genus. If X⊆S\Δ(F) for some S∈Sat(F), then we see that there exists the least element of Sat(F) containing X. This element is denoted by Sat(F)[X]. If S∈Sat(F), then we define the Sat(F)-rank of S as the minimum of {cardinality(X)∣S=Sat(F)[X]}. In this paper, we present an algorithm to compute all the elements of Sat(F) with a given Sat(F)-rank.
Determinants of Chromatin Organization in Aging and Cancer—Emerging Opportunities for Epigenetic Therapies and AI Technology
Authors: Castilho Rogerio M. , Castilho Leonard S. , Palomares Bruna H. , Squarize Cristiane H.
Published: June 2024

This review article critically examines the pivotal role of chromatin organization in gene regulation, cellular differentiation, disease progression and aging. It explores the dynamic between the euchromatin and heterochromatin, coded by a complex array of histone modifications that orchestrate essential cellular processes. We discuss the pathological impacts of chromatin state misregulation, particularly in cancer and accelerated aging conditions such as progeroid syndromes, and highlight the innovative role of epigenetic therapies and artificial intelligence (AI) in comprehending and harnessing the histone code toward personalized medicine. In the context of aging, this review explores the use of AI and advanced machine learning (ML) algorithms to parse vast biological datasets, leading to the development of predictive models for epigenetic modifications and providing a framework for understanding complex regulatory mechanisms, such as those governing cell identity genes. It supports innovative platforms like CEFCIG for high-accuracy predictions and tools like GridGO for tailored ChIP-Seq analysis, which are vital for deciphering the epigenetic landscape. The review also casts a vision on the prospects of AI and ML in oncology, particularly in the personalization of cancer therapy, including early diagnostics and treatment optimization for diseases like head and neck and colorectal cancers by harnessing computational methods, AI advancements and integrated clinical data for a transformative impact on healthcare outcomes.
Cosmic Whirl: Navigating the Comet Trail in DNA: H2AX Phosphorylation and the Enigma of Uncertain Significance Variants
Authors: Ustun Yilmaz Sevdican , Agaoglu Nihat Bugra , Manto Karin , Muftuoglu Meltem , Özbek Ugur
Published: June 2024

Pathogenic variations in the BRCA2 gene have been detected with the development of next-generation sequencing (NGS)-based hereditary cancer panel testing technology. It also reveals an increasing number of variants of uncertain significance (VUSs). Well-established functional tests are crucial to accurately reclassifying VUSs for effective diagnosis and treatment. We retrospectively analyzed the multi-gene cancer panel results of 922 individuals and performed in silico analysis following ClinVar classification. Then, we selected five breast cancer-diagnosed patients’ missense BRCA2 VUSs (T1011R, T1104P/M1168K, R2027K, G2044A, and D2819) for reclassification. The effects of VUSs on BRCA2 function were analyzed using comet and H2AX phosphorylation (γH2AX) assays before and after the treatment of peripheral blood mononuclear cells (PBMCs) of subjects with the double-strand break (DSB) agent doxorubicin (Dox). Before and after Dox-induction, the amount of DNA in the comet tails was similar in VUS carriers; however, notable variations in γH2AX were observed, and according to combined computational and functional analyses, we reclassified T1001R as VUS-intermediate, T1104P/M1168K and D2819V as VUS (+), and R2027K and G2044A as likely benign. These findings highlight the importance of the variability of VUSs in response to DNA damage before and after Dox-induction and suggest that further investigation is needed to understand the underlying mechanisms.
Gut Microbiota, Human Blood Metabolites, and Esophageal Cancer: A Mendelian Randomization Study
Authors: Li Xiuzhi , Xu Bingchen , Yang Han , Zhu Zhihua
Published: June 2024

Background: Unbalances in the gut microbiota have been proposed as a possible cause of esophageal cancer (ESCA), yet the exact causal relationship remains unclear. Purpose: To investigate the potential causal relationship between the gut microbiota and ESCA with Mendelian randomization (MR) analysis. Methods: Genome-wide association studies (GWASs) of 207 gut microbial taxa (5 phyla, 10 classes, 13 orders, 26 families, 48 genera, and 105 species) and 205 gut microbiota metabolic pathways conducted by the Dutch Microbiome Project (DMP) and a FinnGen cohort GWAS of esophageal cancer specified the summary statistics. To investigate the possibility of a mediation effect between the gut microbiota and ESCA, mediation MR analyses were performed for 1091 blood metabolites and 309 metabolite ratios. Results: MR analysis indicated that the relative abundance of 10 gut microbial taxa was associated with ESCA but all the 12 gut microbiota metabolic pathways with ESCA indicated no statistically significant association existing. Two blood metabolites and a metabolite ratio were discovered to be mediating factors in the pathway from gut microbiota to ESCA. Conclusion: This research indicated the potential mediating effects of blood metabolites and offered genetic evidence in favor of a causal correlation between gut microbiota and ESCA.
Genetic Differentiation of Reed Canarygrass (Phalaris arundinacea L.) within Eastern Europe and Eurasia
Authors: Anderson Neil O. , Krokaitė-Kudakienė Edvina , Jocienė Lina , Rekašius Tomas , Chernyagina Olga A. , Paulauskas Algimantas , Kupčinskienė Eugenija
Published: June 2024

Worldwide molecular research of economically important Phalaris arundinacea (Poaceae) is mainly focused on the invasions of this species from Europe to North America. Until the present study, the genetic diversity of the P. arundinacea had not been studied across the Baltic countries. The objective of this research is to evaluate the diversity of Lithuanian populations of P. arundinacea at simple sequence repeat (SSR) loci comparatively among populations of the Baltic countries, Luxembourg, and the Russian Far East (Eurasian), evaluating differentiation between Lithuanian populations and ornamental accessions, and relating these with environmental features. For six selected Lithuanian river basin populations, GBS low density SNPs were used to determine genetic diversity. Bayesian analysis showed that Eurasian populations of Phalaris arundinacea consist of two gene clusters. Statistically significant genetic differentiation among European and Eurasian populations was documented. Lithuanian genotypes growing naturally along rivers are genetically distinct from cultivated ornamentals. GBS-SNPs divided the six selected Nemunas river basins into three distinct groups with one, two, or three rivers in separate groupings for genetic diversity. Genetic diversity is primarily within, rather than among, Lithuanian, eastern European, and Eurasian populations of P. arundinacea across the continent. Thus, restoration efforts would benefit from local population seed origination.
Core Competencies for Gerontogeriatric Nursing: A Validation Study
Authors: Catalão Maria José , Arco Helena , Carrajola Nuno , Almeida Maria de Lurdes , Neves Hugo , Tavares João
Published: June 2024

Background: As the aging population grows, facing multifaceted health challenges and escalating care costs, equipping newly graduated nurses with the requisite skills for high-quality gerontogeriatric care becomes crucial. This study assesses the psychometric properties of a Gerontogeriatric Competency (GGC) scale to evaluate the competencies of newly graduated registered nurses (RNs). Methods: Using a convenience sampling approach, a nationwide, observational prospective cohort study was conducted among 272 newly graduated RNs. The evaluation framework included a sociodemographic questionnaire, three groups of questions targeting gerontogeriatric nursing education aspects, and the GGC scale, with 64 competencies. Construct validity (via confirmatory factor analysis), known-group validity and reliability (assessed by Cronbach’s α) were examined. Results: The confirmatory factor analysis (CFA) showed an adequate index fit: the ratio of chi-square to degrees of freedom (χ2/df) = 2.785, the goodness-of-fit index (GFI) = 0.579, confirmatory fit index (CFI) = 0.864, the parsimony goodness-of-fit index (PGFI) = 0.526, the parsimony confirmatory-of-fit index (PCFI) = 0.809, the root mean square error of approximation (RMSEA) = 0.087, and the modified expected cross-validation index (MECVI) = 24.418. Differences were observed in gerontogeriatric competencies based on curriculum inclusion, self-confidence, knowledge in caring for older adults, and satisfaction with the nursing program content. The Cronbach’s α coefficient was 0.992 for the overall scale and ranged from 0.935 to 0.983 for the GGC dimensions. Conclusions: The GGC scale is a valid and reliable tool for assessing the gerontogeriatric competencies of new graduate RNs, highlighting its potential to enhance education, training, and, ultimately, the quality of care provided to the older population.
Exploration of the Hungarian Version of Test Your Memory in General Practice: A Cross-Sectional Correlational Study of a Convenience Sample of Middle-Aged and Older Adults
Authors: Garbóczy Szabolcs , Mohos András , Égerházi Anikó , Szemán-Nagy Anita , Zsuffa János András , Heim Szilvia , Rekenyi Viktor , Kolozsvári László Róbert
Published: June 2024

Background: Dementia is a significant health issue worldwide. Mild cognitive impairment (MCI) can transform into dementia over time. General practitioners (GPs) may be the first to notice the cognitive deficit; therefore, it is crucial for them to have access to a screening test that can be administered quickly and efficiently. We explored the Hungarian version of the Test Your Memory self-administered dementia screening test in general practice for the early detection of dementia and cognitive impairment. Methods: In the four Hungarian cities with medical universities, 368 patients over the age of 50 attending GPs filled out the questionnaire within the framework of our cross-sectional study. Results: The total scores of the test showed a significant correlation with education and type of occupation and a significant negative correlation with age. The results of this research showed that the clock drawing and recall subtest scores deteriorate at the earliest age. Conclusion: The test can be filled out in a GP’s office easily and two of its subtests can raise the possibility that patients may need further assessment, especially if they have symptoms, at an earlier age than the other subtests.
Exercise, Dietary Habits, and Defecatory Dysfunction in Patients Living with Colorectal Cancer: A Preliminary Quantitative Study
Authors: Nakagawa Hiromi , Sasai Hiroyuki , Kato Yoshimi , Matsumoto Shinobu , Tanaka Kiyoji
Published: June 2024

This study investigated the association of exercise and dietary habits with defecatory dysfunction in patients living with colorectal cancer. We recruited 61 adult patients who had undergone surgery within the past 20 years and attended outpatient clinics at designated cancer hospitals in Japan. Defecatory dysfunction was defined as any symptom caused by issues with colon and anal function, including fecal incontinence, evacuation difficulties, frequent stools, diarrhea, and constipation. Exercise and dietary habits were assessed via a quantitative questionnaire survey. Postoperative defecatory dysfunction occurred in all the patients. Multivariate analysis revealed no association between exercise habits and defecatory dysfunction; however, dietary fiber intake ≥4 times a week was associated with frequent stools (adjusted odds ratio, 5.11; 95% confidence interval, 1.10, 23.70). These findings suggest a need to alleviate defecatory dysfunction by improving one’s dietary habits. Interventions aimed at alleviating defecatory dysfunction by improving the dietary habits in patients living with colorectal cancer are needed.
CAR-T Therapy in Multiple Myeloma: Looking Beyond
Authors: Maiorana Gianluca , Antolino Giusy , La Verde Giacinto , Tafuri Agostino
Published: June 2024

Multiple Myeloma is a hematological neoplasm that, over the recent few years, has benefited from numerous therapeutic options. Among the latter, CAR-T stands out as the most recent and one of the most promising treatments currently available. Despite its recent introduction, multiple CAR-T products have already been approved, and research regarding cellular therapy is rapidly increasing. We conducted a comprehensive search and review of the available literature, including published studies and abstracts from recent meetings (ASH, ASCO, ASTCT, IMS), regarding Multiple Myeloma and CAR-T therapy. We describe the discovery and research regarding promising targets like the B-Cell Maturation Antigen (BCMA) and others, the origin and nature of CAR-T cells, and the recent introduction of anti-BCMA CAR-Ts Idecabtagene-vicleucel and Ciltacabtagene-autoleucel, which are currently the only approved CAR-T products for MM. Additionally, we discuss non-BCMA-targeting CAR-Ts and their clinical implications. Given the significant impact of cellular therapy, we provide an overview of its limitations and possible adverse implications, as well as related resistance mechanisms. Finally, we describe the current research aimed at improving CAR-T therapy in MM, including structural innovations and new therapeutic approaches, such as in the earlier lines of treatment and maintenance therapy.
Multi-Sensor Geomatic Techniques for the 3D Documentation and Virtual Repositioning of Elements of the Church of S. Miguel (Jaén, Spain)
Authors: Mozas-Calvache Antonio Tomás , Gómez-López José Miguel , Pérez-García José Luis , Vico-García Diego , Barba-Colmenero Vicente , Fernández-Ordóñez Alberto
Published: June 2024

This study describes the methodology and main results obtained after applying several geomatic techniques, based on the fusion of data acquired by several sensors, to document the recovery works carried out in an abandoned church. A century ago, the façade was moved to a museum to ensure its preservation. In addition to documentary purposes, a secondary goal is the virtual repositioning of a model of this element on that of the church. The method takes advantage of the potential of each technique, considering the acquisition of geometry based mainly on laser scanning techniques and radiometry on photogrammetry. The results include 3D models and orthoimages, which are used to perform a stratigraphic study. The 3D model of the façade has been repositioned in the general one, considering common geometries previously fitted in both models and repeating part of the photogrammetric process, using masks to define the image areas related to the church and the façade. Therefore, we obtained a 3D model with the façade included in it. This procedure has demonstrated its feasibility despite the existence of different environmental conditions in both areas. Using these results, we have also developed a BIM to allow for the management of future restoration works.
Hospital Mortality and Morbidity in Diabetic Patients with COVID-19: A Retrospective Analysis from the UAE
Authors: Mohamed Yehia S. , Mukhtar Mamoun , Elmalti Akrem , Kheirallah Khalid , Panigrahi Debadatta , Abu-rish Eman Y. , Bani Ibrahim , Nasor Eiman Mohamed , Ahmed Wafa , Alzoubi Abdallah
Published: June 2024

Background: Although we are four years into the pandemic, there is still conflicting evidence regarding the clinical outcomes of diabetic patients hospitalized with COVID-19. The primary objective of this study was to evaluate the in-hospital mortality and morbidity of diabetic versus nondiabetic patients hospitalized with COVID-19 in the Northern UAE Emirates. Methods: A retrospective analysis was performed on clinical data from patients with or without diabetes mellitus (DM) who were admitted to the isolation hospital with COVID-19 during the first and second waves of the disease (March 2020 to April 2021). The assessed endpoints were all-cause in-hospital mortality, length of hospitalization, intensive care unit (ICU) admission, and mechanical ventilation. Results: A total of 427 patients were included in the analysis, of whom 335 (78.5%) had DM. Compared to nondiabetics, diabetic COVID-19 patients had a significantly longer in-hospital stay (odds ratio (OR) = 2.35; 95% confidence interval (CI) = 1.19–4.62; p = 0.014), and a significantly higher frequency of ICU admission (OR = 4.50; 95% CI = 1.66–7.34; p = 0.002). The need for mechanical ventilation was not significantly different between the two groups (OR: distorted estimates; p = 0.996). Importantly, the overall in-hospital mortality was significantly higher among diabetic patients compared to their nondiabetic counterparts (OR = 2.26; 95% CI = 1.08–4.73; p = 0.03). Conclusion: DM was associated with a more arduous course of COVID-19, including a higher mortality rate, a longer overall hospital stay, and a higher frequency of ICU admission. Our results highlight the importance of DM control in COVID-19 patients to minimize the risk of detrimental clinical outcomes.
Characteristics of Older Adults with Alzheimer’s Disease Who Were Hospitalized during the COVID-19 Pandemic: A Secondary Data Analysis
Authors: Wang Dingyue , Hendrix Cristina C. , Lee Youran , Noval Christian , Crego Nancy
Published: June 2024

We aim to investigate the relationships between the population characteristics of patients with Alzheimer’s Disease (AD) and their Healthcare Utilization (HU) during the COVID-19 pandemic. Electronic health records (EHRs) were utilized. The study sample comprised those with ICD-10 codes G30.0, G30.1, G30.8, and G30.9 between 1 January 2020 and 31 December 2021. Pearson’s correlation and multiple regression were used. The analysis utilized 1537 patient records with an average age of 82.20 years (SD = 7.71); 62.3% were female. Patients had an average of 1.64 hospitalizations (SD = 1.18) with an average length of stay (ALOS) of 7.45 days (SD = 9.13). Discharge dispositions were primarily home (55.1%) and nursing facilities (32.4%). Among patients with multiple hospitalizations, a negative correlation was observed between age and both ALOS (r = −0.1264, p = 0.0030) and number of hospitalizations (r = −0.1499, p = 0.0004). Predictors of longer ALOS included male gender (p = 0.0227), divorced or widowed (p = 0.0056), and the use of Medicare Advantage and other private insurance (p = 0.0178). Male gender (p = 0.0050) and Black race (p = 0.0069) were associated with a higher hospitalization frequency. We recommend future studies including the co-morbidities of AD patients, larger samples, and longitudinal data.
Adaptation and Validation of the S-NutLit Scale to Assess Nutritional Literacy in the Peruvian Population
Authors: Chilón-Troncos Rony Francisco , García-Salirrosas Elizabeth Emperatriz , Millones-Liza Dany Yudet , Villar-Guevara Miluska
Published: June 2024

Maintaining good dietary practices is a factor that allows a better quality of life; therefore, it is necessary to promote health via the fundamental tool of nutritional literacy. In this context, this study aims to evaluate nutritional literacy in Peru through the S-NutLit tool, which is composed of two dimensions. The scale, composed of 11 items, was applied to 396 Peruvian adults. According to the evaluation of the indicators, an acceptable reliability was found, as was a model fit with excellent estimation according to its indicators (CMIN/DIF 2.559; IFC 0.965; SRMR 0.043; RMSEA 0.063; and PClose 0.070). In this way, we seek to reinforce health promotion activities through a nutritional literacy diagnosis, which, due to its characteristics, can be self-administered and used by health entities and other entities in general that are interested in knowing the eating practices of an individual, which undoubtedly leads to good health.
Using the Nominal Group Technique to Inform Approaches for Enhancing Men’s Utilization of Sexual and Reproductive Health Services
Authors: Nyalela Mpumelelo , Dlungwane Thembelihle
Published: June 2024

Sexual and reproductive health (SRH) services’ underutilization by men remains a global public health challenge. SRH problems constitute major health challenges in that they form almost one-seventh of the disease burden and contribute to higher and earlier morbidity among men. We, therefore, invited subject matter experts to collaborate in co-creating intervention strategies to enhance men’s utilization of SRH services. We employed the nominal group technique (NGT) for data collection. The NGT is a structured method that involves gathering a group of people to discuss a problem for the purpose of achieving a group consensus and planning actions for the selected problem. The participants who were purposively sampled included researchers, scientists, academics, clinicians, and policymakers. The participants suggested the need to improve men’s knowledge, provide healthcare resources such as equipment, medical supplies, and SRH-trained male healthcare workers, deal with healthcare workers’ negative attitudes through training and capacitation, and destigmatize socially constructed gender norms that deter men from seeking medical help. These important intervention strategies can be implemented to encourage men’s use of SRH services. Men’s current underutilization of SRH services requires the urgent implementation of evidence-based interventions. Collaborating with SRH experts in identifying appropriate intervention strategies can assist program managers and policymakers in designing SRH services tailored to men’s sexual health needs.
Mitigating Identity-Related Anxiety through Humor and Immersive Storytelling with 360-Degree Video in Virtual Reality: A Study on Microaggressions’ Mental Health Effects
Authors: Yan Changmin , Eno Alan , Wagler Adam
Published: June 2024

Background: Microaggressions are subtle slights that can cause significant psychological distress among marginalized groups. Few studies have explored interventions that might mitigate these effects. Objective: This study aimed to investigate if and how humor-infused immersive storytelling via virtual reality (VR) could reduce identity-related psychological distress caused by microaggressions. Methods: Using a community-based participatory research approach, we developed a 7-min 360-degree VR film depicting scenarios of microaggressions across various identities. Forty-six college students participated in a controlled study where they were exposed to this immersive VR experience. We measured identity-related psychological anxiety, character identification, perceived humor, and perceived psychological presence. Results: The findings demonstrated a significant anxiety reduction following the VR intervention, supporting the efficacy of humor-infused storytelling in alleviating the impact of microaggressions. Character identification significantly predicted anxiety reduction, while perceived humor and psychological presence did not directly influence anxiety reduction but indirectly contributed through enhanced character identification. Conclusions: Humor-infused immersive storytelling, facilitated by VR, effectively reduces identity-related psychological distress primarily through character identification. The structural equation modeling results emphasize the importance of integrating humor and psychological presence to enhance character connection, advocating for a balanced approach that combines traditional narrative elements with technological innovations in health interventions aimed at combating the adverse psychological effects of microaggressions.
Implementation of a Comprehensive and Personalised Approach for Older People with Psychosocial Frailty in Valencia (Spain): Study Protocol for a Pre–Post Controlled Trial
Authors: Fernández-Salido Mirian , Alhambra-Borrás Tamara , Garcés-Ferrer Jorge
Published: June 2024

With ageing, the risk of frailty increases, becoming a common condition that exposes older people to an increased risk of multiple adverse health outcomes. In Valencia (Spain), the ValueCare project develops and applies a value-based care approach that addresses the multidimensional nature of frailty by implementing integrated and personalized care to tackle psychosocial frailty. A pre–post controlled design with a baseline measurement at inclusion, at the end of implementation and a follow-up measurement after 6 months of intervention. In Valencia (Spain), 120 participants over 65 years of age are recruited from primary care centres to receive the ValueCare comprehensive and personalised care plan according to the results and are compared with 120 participants receiving “usual care”. An assessment questionnaire is designed using validated instruments, and a personalised care plan is developed specifically for each participant based on the results obtained. The study protocol has been registered under the ISRCTN registration number ISRCTN25089186. Addressing frailty as a multidimensional and multifactorial risk condition requires the development and implementation of comprehensive assessments and care. In this context, this study will provide new insights into the feasibility and effectiveness of a value-based methodology for integrated care supported by ICT for older people experiencing frailty.
Myelin Oligodendrocyte Glycoprotein (MOG)35–55 Mannan Conjugate Induces Human T-Cell Tolerance and Can Be Used as a Personalized Therapy for Multiple Sclerosis
Authors: Rodi Maria , de Lastic Anne-Lise , Panagoulias Ioannis , Aggeletopoulou Ioanna , Kelaidonis Kostas , Matsoukas John , Apostolopoulos Vasso , Mouzaki Athanasia
Published: June 2024

We have previously performed preclinical studies with the oxidized mannan-conjugated peptide MOG35–55 (OM-MOG35–55) in vivo (EAE mouse model) and in vitro (human peripheral blood) and demonstrated that OM-MOG35–55 suppresses antigen-specific T cell responses associated with autoimmune demyelination. Based on these results, we developed different types of dendritic cells (DCs) from the peripheral blood monocytes of patients with multiple sclerosis (MS) or healthy controls presenting OM-MOG35–55 or MOG-35–55 to autologous T cells to investigate the tolerogenic potential of OM-MOG35–55 for its possible use in MS therapy. To this end, monocytes were differentiated into different DC types in the presence of IL-4+GM-CSF ± dexamethasone (DEXA) ± vitamin D3 (VITD3). At the end of their differentiation, the DCs were loaded with peptides and co-cultured with T cells +IL-2 for 4 antigen presentation cycles. The phenotypes of the DC and T cell populations were analyzed using flow cytometry and the secreted cytokines using flow cytometry or ELISA. On day 8, the monocytes had converted into DCs expressing the typical markers of mature or immature phenotypes. Co-culture of T cells with all DC types for 4 antigen presentation cycles resulted in an increase in memory CD4+ T cells compared to memory CD8+ T cells and a suppressive shift in secreted cytokines, mainly due to increased TGF-β1 levels. The best tolerogenic effect was obtained when patient CD4+ T cells were co-cultured with VITD3-DCs presenting OM-MOG35–55, resulting in the highest levels of CD4+PD-1+ T cells and CD4+CD25+Foxp3+ Τ cells. In conclusion, the tolerance induction protocols presented in this work demonstrate that OM-MOG35–55 could form the basis for the development of personalized therapeutic vaccines or immunomodulatory treatments for MS.