bluuebunny/crossref_metadata_2025 huggingface dataset:
Ransomware-Resilient Self-Healing XML Documents
Authors: Al-Dwairi Mahmoud , Shatnawi Ahmed S. , Al-Khaleel Osama , Al-Duwairi Basheer
Published: April 2022

In recent years, various platforms have witnessed an unprecedented increase in the number of ransomware attacks targeting hospitals, governments, enterprises, and end-users. The purpose of this is to maliciously encrypt documents and files on infected machines, depriving victims of access to their data, whereupon attackers would seek some sort of a ransom in return for restoring access to the legitimate owners; hence the name. This cybersecurity threat would inherently cause substantial financial losses and time wastage for affected organizations and users. A great deal of research has taken place across academia and around the industry to combat this threat and mitigate its danger. These ongoing endeavors have resulted in several detection and prevention schemas. Nonetheless, these approaches do not cover all possible risks of losing data. In this paper, we address this facet and provide an efficient solution that would ensure an efficient recovery of XML documents from ransomware attacks. This paper proposes a self-healing version-aware ransomware recovery (SH-VARR) framework for XML documents. The proposed framework is based on the novel idea of using the link concept to maintain file versions in a distributed manner while applying access-control mechanisms to protect these versions from being encrypted or deleted. The proposed SH-VARR framework is experimentally evaluated in terms of storage overhead, time requirement, CPU utilization, and memory usage. Results show that the snapshot size increases proportionately with the original size; the time required is less than 120 ms for files that are less than 1 MB in size; and the highest CPU utilization occurs when using the bzip2. Moreover, when the zip and gzip are used, the memory usage is almost fixed (around 6.8 KBs). In contrast, it increases to around 28 KBs when the bzip2 is used.
Applications of Plant Protein in the Dairy Industry
Authors: Hu Ge-Ge , Liu Jing , Wang Yi-Hui , Yang Zhen-Nai , Shao Hong-Bo
Published: April 2022

In recent years, a variety of double protein dairy products have appeared on the market. It is a dairy product made by replacing parts of animal protein with plant protein and then using certain production methods. For some countries with limited milk resources, insufficient protein intake and low income, double protein dairy products have a bright future. More and more studies have found that double protein dairy products have combined effects which can alleviate the relatively poor functional properties of plant protein, including solubility, foaming, emulsifying and gelling. In addition, the taste of plant protein has been improved. This review focuses on the current state of research on double protein dairy products. It covers some salient features in the science and technology of plant proteins and suggests strategies for improving their use in various food applications. At the same time, it is expected that the fermentation methods used for those traditional dairy products as well as other processing technologies could be applied to produce novelty foods based on plant proteins.
Preliminary Results about Lamb Meat Tenderness Based on the Study of Novel Isoforms and Alternative Splicing Regulation Pathways Using Iso-seq, RNA-seq and CTCF ChIP-seq Data
Authors: Yuan Zehu , Ge Ling , Zhang Weibo , Lv Xiaoyang , Wang Shanhe , Cao Xiukai , Sun Wei
Published: April 2022

Tenderness is an important indicator of meat quality. Novel isoforms associated with meat tenderness and the role of the CCCTC-binding factor (CTCF) in regulating alternative splicing to produce isoforms in sheep are largely unknown. The current project studied six sheep from two crossbred populations (Dorper × Hu × Hu, DHH and Dorper × Dorper × Hu, DDH) with divergent meat tenderness. Pooled Iso-seq data were used to annotate the sheep genomes. Then, the updated genome annotation and six RNA-seq data were combined to identify differentially expressed isoforms (DEIs) in muscles between DHH and DDH. These data were also combined with peaks detected from CTCF ChIP-seq data to investigate the regulatory role of CTCF for the alternative splicing. As a result, a total of 624 DEIs were identified between DDH and DHH. For example, isoform 7.524.18 transcribed from CAPN3 may be associated with meat tenderness. In addition, a total of 86 genes were overlapped between genes with transcribed DEIs and genes in differential peaks identified by CTCF ChIP-seq. Among these overlapped genes, ANKRD23 produces different isoforms which may be regulated by CTCF via methylation. As preliminary research, our results identified novel isoforms associated with meat tenderness and revealed the possible regulating mechanisms of alternative splicing to produce isoforms.
Application of Pulsed Electric Field as a Pre-Treatment for Subcritical Water Extraction of Quercetin from Onion Skin
Authors: Kim Han-Sol , Ko Min-Jung , Park Chan-Ho , Chung Myong-Soo
Published: April 2022

Pulsed electric field (PEF) treatment promotes the electroporation of biological membranes, and if included as a pre-treatment, could improve the extraction of certain constituents therefrom. The aim of this study was to determine the optimal extraction conditions for extracting the flavonoid quercetin from dried onion skin and to establish whether the yield could be enhanced by combining PEF pre-treatment with an eco-friendly extraction method that uses subcritical water extraction (SWE). Samples of onion skin were treated with PEF under conditions of varying electric field strength (0.5–2.5 kV/cm) and duration (5–120 s). SWE was then performed with an extraction time of 15 min and at temperatures ranging from 105 °C to 185 °C. Among the conditions tested, the yield of total quercetin was the highest after pretreatment with PEF at 2.5 kV/cm for 15 s, followed by SWE at 145 °C for 15 min (yield 19.25 ± 0.77 mg/g dried onion skin, mean ± standard deviation). Pretreatment with PEF improved the yield of total quercetin extracted by 33.22% compared with the PEF-untreated samples. These findings demonstrate that pretreatment of onion skin with PEF has the potential to improve flavonoid extraction.
Total Anthocyanin Content of Strawberry and the Profile Changes by Extraction Methods and Sample Processing
Authors: Taghavi Toktam , Patel Hiral , Akande Omololu E. , Galam Dominique Clark A.
Published: April 2022

Anthocyanins are the primarily pigments in many flowers, vegetables, and fruits and play a critical role in human and plant health. They are polyphenolic pigments that are soluble in water and usually quantified by spectrophotometric methods. The two main methods that quantify anthocyanins are pH differential and organic solvent-based methods. Our hypothesis was that these methods extract different anthocyanin profiles. Therefore, this experiment was designed to identify anthocyanin profiles that are extracted by pH differential and organic solvent-based methods and observe their total anthocyanin content from strawberries. Six methods were tested in this experiment to quantify and profile anthocyanins in strawberry fruits by spectrophotometry and Ultra High Performance Liquid Chromatography (UHPLC) respectively. Four methods used organic solvents (methanol, and chloroform-methanol) in different combinations. The next two methods were pH differential and a combination of organic solvent and the pH differential method. The results suggest that acidified chloroform-methanol extracted the highest anthocyanin content compared to water-based solvents. Methanol-water based solvents also performed better than methanol alone, because both methanol and water may extract different profiles of anthocyanins. Water-based extracts had the greatest absorbance at a lower wavelength (498 nm), followed by methanol (508 nm), and chloroform (530 nm). Chloroform-methanol solvent with higher pH (3.0) extracted pelargonidin as the main anthocyanin, while methanol and water-based solvents (with lower pH 1.0–2.0) extracted delphinidin as their main anthocyanin as identified by UHPLC. Therefore, chloroform-methanol and methanol-water solvents were the best solvents for extracting anthocyanins from strawberries. Also, freeze-dried strawberries had higher anthocyanin contents compared to fresh or frozen samples.
The Bacterial Microbiota of Edible Insects Acheta domesticus and Gryllus assimilis Revealed by High Content Analysis
Authors: Aleknavičius Dominykas , Lukša Juliana , Strazdaitė-Žielienė Živilė , Servienė Elena
Published: April 2022

In the concept of novel food, insects reared under controlled conditions are considered mini livestock. Mass-reared edible insect production is an economically and ecologically beneficial alternative to conventional meat gain. Regarding food safety, insect origin ingredients must comply with food microbial requirements. House crickets (Acheta domesticus) and Jamaican field crickets (Gryllus assimilis) are preferred insect species that are used commercially as food. In this study, we examined cricket-associated bacterial communities using amplicon-based sequencing of the 16S ribosomal RNA gene region (V3–V4). The high taxonomic richness of the bacterial populations inhabiting both tested cricket species was revealed. According to the analysis of alpha and beta diversity, house crickets and Jamaican field crickets displayed significantly different bacterial communities. Investigation of bacterial amplicon sequence variants (ASVs) diversity revealed cricket species as well as surface and entire body-associated bacterial assemblages. The efficiency of crickets processing and microbial safety were evaluated based on viable bacterial counts and identified bacterial species. Among the microorganisms inhabiting both tested cricket species, the potentially pathogenic bacteria are documented. Some bacteria representing identified genera are inhabitants of the gastrointestinal tract of animals and humans, forming a normal intestinal microflora and performing beneficial probiotic functions. The novel information on the edible insect-associated microbiota will contribute to developing strategies for cricket processing to avoid bacteria-caused risks and reap the benefits.
Numerical Solutions of Variable-Coefficient Fractional-in-Space KdV Equation with the Caputo Fractional Derivative
Authors: Han Che , Wang Yu-Lan
Published: April 2022

In this paper, numerical solutions of the variable-coefficient Korteweg-De Vries (vcKdV) equation with space described by the Caputo fractional derivative operator is developed. The propagation and interaction of vcKdV equation in different cases, such as breather soliton and periodic suppression soliton, are numerically simulated. Especially, the Fourier spectral method is used to solve the fractional-in-space vcKdV equation with breather soliton. From numerical simulations and compared with other methods, it can be easily seen that our method has low computational complexity and higher precision.
Effect of Vibrations, Displacement, Pressure, Temperature and Humidity on the Resistance and Impedance of the Shockproof Resistors Based on Rubber and Jelly (NiPc–CNT–Oil) Composites
Authors: Chani Muhammad Tariq Saeed , Karimov Khasan S. , Asiri Abdullah M. , Rahman Mohammed M. , Kamal Tahseen
Published: April 2022

Here, we present the design, fabrication and characterization of shockproof rubber–jelly (NiPc–CNT–oil) composite-based resistors. To fabricate the resistors, gels of CNT and NiPc with edible oil were prepared and deposited on a flexible rubber substrate using rubbing-in technique. The devices’ resistance and impedance were investigated under the effect of pressure, displacement, humidity, temperature and mechanical vibrations. The resistance and the impedance decreased, on average, by 1.08 times under the effect of pressure (up to 850 gf/cm2) and by 1.04 times under the effect of displacement (up to 50 µm). Accordingly, upon increasing the humidity from 60% to 90% RH, the resistance and impedance decreased by up to 1.04 times, while upon increasing the temperature from 25 °C to 43 °C, the resistance and impedances also decreased by up to 1.05 times. Moreover, under the effect of vibration, a decrease in resistance and impedance, by up to 1.03 times, was observed. The investigated samples can potentially be used as prototypes for the development of shockproof jelly electronic-based devices in particular resistors. The technological achievement in the fabrication of these devices is the use of edible organic oil, which allows for the fabrication of uniform jelly films of organic materials that cannot be realized simply by mixing “dry” ingredients. Especially, we highlight that edible organic oil is environmentally friendly, unlike some other inorganic oils that are used in practice.
Influence of Levan on the Thermally Induced Gel Formation of β-Lactoglobulin
Authors: Hundschell Christoph S. , Brühan Juliane , Anzmann Theresa , Kohlus Reinhard , Wagemans Anja M.
Published: April 2022

In this study, the influence of levan on the phase behavior and the thermally induced gelation of the mixed β-lactoglobulin—levan gels as a function of polymer content, molecular weight and ionic strength was characterized. For this purpose, rheology was used to study the mechanical properties of the gels and the water binding of the network structure was investigated by time domain nuclear magnetic resonance. Phase behavior and network type were analyzed by optical observation and electron microscopy. Levan enhanced the aggregation and gel formation of β-lg due to segregative forces between the polymer species. Segregation was caused by the excluded volume effect and was more pronounced at lower ionic strength, higher levan contents and higher levan molecular weights. The presence of levan increased the water binding of the gel networks. However, this effect decreased with increasing levan content. At high ionic strength and high levan content, phase separated gels were formed. While segregative forces enhanced network formation, and therefore, increased the gel strength of mixed gels at low ionic strength, levan had also antagonistic effects on the network formation at high ionic strength and high polymer contents.
Using an Unsupervised Clustering Model to Detect the Early Spread of SARS-CoV-2 Worldwide
Authors: Li Yawei , Liu Qingyun , Zeng Zexian , Luo Yuan
Published: April 2022

Deciphering the population structure of SARS-CoV-2 is critical to inform public health management and reduce the risk of future dissemination. With the continuous accruing of SARS-CoV-2 genomes worldwide, discovering an effective way to group these genomes is critical for organizing the landscape of the population structure of the virus. Taking advantage of recently published state-of-the-art machine learning algorithms, we used an unsupervised deep learning clustering algorithm to group a total of 16,873 SARS-CoV-2 genomes. Using single nucleotide polymorphisms as input features, we identified six major subtypes of SARS-CoV-2. The proportions of the clusters across the continents revealed distinct geographical distributions. Comprehensive analysis indicated that both genetic factors and human migration factors shaped the specific geographical distribution of the population structure. This study provides a different approach using clustering methods to study the population structure of a never-seen-before and fast-growing species such as SARS-CoV-2. Moreover, clustering techniques can be used for further studies of local population structures of the proliferating virus.
TSA Activates Pluripotency Factors in Porcine Recloned Embryos
Authors: Feng Tao , Qi Xiaolan , Zou Huiying , Ma Shuangyu , Yu Dawei , Gao Fei , Lian Zhengxing , Wu Sen , Du Xuguang
Published: April 2022

Animal cloning is of great importance to the production of transgenic and genome-edited livestock. Especially for multiple gene-editing operations, recloning is one of the most feasible methods for livestock. In addition, a multiple-round cloning method is practically necessary for animal molecular breeding. However, cloning efficiency remains extremely low, especially for serial cloning, which seriously impedes the development of livestock breeding based on genome editing technology. The incomplete reprogramming and failure in oocyte activation of some pluripotent factors were deemed to be the main reason for the low efficiency of animal recloning. Here, to overcome this issue, which occurred frequently in the process of animal recloning, we established a reporter system in which fluorescent proteins were driven by pig OCT4 or SOX2 promoter to monitor the reprogramming process in cloned and recloned pig embryos. We studied the effect of different histone deacetylase (HDAC) inhibitors on incomplete reprogramming. Our results showed that Trichostatin A (TSA) could activate pluripotent factors and significantly enhance the development competence of recloned pig embryos, while the other two inhibitors, valproic acid (VPA) and Scriptaid, had little effect on that. Furthermore, we found no difference in OCT4 mRNA abundance between TSA-treated and untreated embryos. These findings suggest that TSA remarkably improves the reprogramming state of pig recloned embryos by restoring the expression of incompletely activated pluripotent genes OCT4 and SOX2.
Transcriptomic and Proteomic Analysis of Marine Nematode Litoditis marina Acclimated to Different Salinities
Authors: Xie Yusu , Zhang Liusuo
Published: April 2022

Salinity is a critical abiotic factor for all living organisms. The ability to adapt to different salinity environments determines an organism’s survival and ecological niches. Litoditis marina is a euryhaline marine nematode widely distributed in coastal ecosystems all over the world, although numerous genes involved in its salinity response have been reported, the adaptive mechanisms underlying its euryhalinity remain unexplored. Here, we utilized worms which have been acclimated to either low-salinity or high-salinity conditions and evaluated their basal gene expression at both transcriptomic and proteomic levels. We found that several conserved regulators, including osmolytes biosynthesis genes, transthyretin-like family genes, V-type H+-transporting ATPase and potassium channel genes, were involved in both short-term salinity stress response and long-term acclimation processes. In addition, we identified genes related to cell volume regulation, such as actin regulatory genes, Rho family small GTPases and diverse ion transporters, which might contribute to hyposaline acclimation, while the glycerol biosynthesis genes gpdh-1 and gpdh-2 accompanied hypersaline acclimation in L. marina. This study paves the way for further in-depth exploration of the adaptive mechanisms underlying euryhalinity and may also contribute to the study of healthy ecosystems in the context of global climate change.
Bioinformatics Analysis Highlight Differentially Expressed CCNB1 and PLK1 Genes as Potential Anti-Breast Cancer Drug Targets and Prognostic Markers
Authors: Fang Leiming , Liu Qi , Cui Hongtu , Zheng Yunji , Wu Chengjun
Published: April 2022

Breast cancer is one of the most common malignant tumors in women worldwide. Early diagnosis, treatment, and prognosis of breast cancer are global challenges. Identification of valid predictive diagnosis and prognosis biomarkers and drug targets are crucial for breast cancer prevention. This study characterizes differentially expressed genes (DEGs) based on the TCGA database by using DESeq2, edgeR, and limma. A total of 2032 DEGs, including 1026 up-regulated genes and 1006 down-regulated genes were screened. Followed with WGCNA, PPI analysis, GEPIA 2, and HPA database verification, thirteen hub genes including CDK1, BUB1, BUB1B, CDC20, CCNB2, CCNB1, KIF2C, NDC80, CDCA8, CENPF, BIRC5, AURKB, PLK1, MAD2L1, and CENPE were obtained, and they may serve as potential therapeutic targets of breast cancer. Especially, overexpression of CCNB1 and PLK1 are strongly associated with the low survival rate of breast cancer patients, demonstrating their potentiality as prognostic markers. Moreover, CCNB1 and PLK1 are highly expressed in all breast cancer stages, suggesting that they could be further studied as potential drug targets. Taken together, our study highlights CCNB1 and PLK1 as potential anti-breast cancer drug targets and prognostic markers.
Evaluating Sexual Dimorphism in Postcranial Elements of Eurasian Extinct Stephanorhinus etruscus (Falconer, 1868) (Mammalia, Rhinocerotidae)
Authors: Faggi Andrea , Pandolfi Luca
Published: April 2022

Sexual dimorphism has been poorly evaluated or investigated in Pleistocene Eurasian Stephanorhinus species, leaving a gap in our knowledge about their morphometric variability. Among the representatives of this genus, S. etruscus is the most abundant species, with several remains collected from Western European localities, allowing us to investigate the presence of sexual dimorphism in the limb bones of this taxon. We considered measurements taken on 45 postcranial variables and three different statistical metrics to identify patterns of bimodality in the dataset. This work represents the first application of sex-combined statistical analysis to a dataset composed of individuals from various European localities. The morphometrical analyses revealed that a relatively weak sexual dimorphism is present in all the considered bones. Larger forelimbs and hindlimbs are interpreted as belonging to adult males of S. etruscus, similarly to what was observed in the modern Sumatran rhino, where males are a little bit larger than females. The recognition of a weak sexual dimorphism in the postcranial bones of S. etruscus increases our understanding of the paleoecology of this extinct taxon. However, only a better study of the morphological and morphometrical variability of the crania of fossil rhinoceroses could deeply contribute to the investigation of the social habits and behavior of these taxa.
Oxygen Isotopes from Apatite of Middle and Late Ordovician Conodonts in Peri-Baltica (The Holy Cross Mountains, Poland) and Their Climatic Implications
Authors: Trela Wiesław , Krzemińska Ewa , Jewuła Karol , Czupyt Zbigniew
Published: April 2022

This report provides oxygen isotopes from apatite of late Middle and Late Ordovician conodonts from the southern Holy Cross Mountains in south-eastern Poland. It was a unique time interval characterised by a significant change in the Ordovician climate, tectonic, and ocean chemistry. In the Middle and early Late Ordovician, the Holy Cross Mountains were located in the mid-latitude climatic zone at the southwestern periphery of Baltica; therefore, the δ18Oapatite values from this region provide new data on the 18O/16O budget in the Ordovician seawater reconstructed mainly from the tropical and subtropical realms. Oxygen isotopes from mixed conodont samples were measured using the SHRIMP IIe/MC ion microprobe in the Polish Geological Institute in Warsaw. The δ18Oapatite values range from 16.75‰VSMOW to 20.66‰VSMOW with an average of 18.48‰VSMOW. The oxygen isotopes from bioapatite of the studied section display an increasing trend, suggesting a progressive decrease in sea-surface temperature roughly consistent with an overall cooling of the Ordovician climate. Two distinctive positive excursions of δ18Oapatite have been reported in the upper Sandbian and middle Katian of the studied section and correlated with cooling events recognised in Baltica. They are interpreted as an isotope temperature proxy of climate changes triggered by a growing continental polar ice cap, but increased δ18Oapatite in the late Sandbian contradicts recently postulated climate warming during that time in subtropical Laurentia.
Why Percussive Massage Therapy Does Not Improve Recovery after a Water Rescue? A Preliminary Study with Lifeguards
Authors: Alonso-Calvete Alejandra , Lorenzo-Martínez Miguel , Pérez-Ferreirós Alexandra , Couso-Bruno Antonio , Carracedo-Rodríguez Eloy , Barcala-Furelos Martín , Barcala-Furelos Roberto , Padrón-Cabo Alexis
Published: April 2022

The aim of this study was to analyze the effects of percussive massage therapy (PMT) on lifeguards’ recovery after a water rescue, in comparison with passive recovery. Methods: A quasi-experimental crossover design was conducted to compare passive recovery (PR) and a PMT protocol. A total of 14 volunteer lifeguards performed a simulated 100 m water rescue and perceived fatigue and blood lactate were measured as recovery variables after the rescue and after the 8-min recovery process. Results: There were no differences between PMT and PR in lactate clearance (p > 0.05), finding in both modalities a small but not significant decrease in blood lactate. In perceived fatigue, both methods decreased this variable significantly (p < 0.001), with no significant differences between them (p > 0.05). Conclusions: PMT does not enhance recovery after a water rescue, in comparison with staying passive. Despite PMT appearing to be adequate for recovery in other efforts, it is not recommended for lifeguards’ recovery after a water rescue.
Vermicompost and Its Derivatives against Phytopathogenic Fungi in the Soil: A Review
Authors: Gudeta Kasahun , Bhagat Ankeet , Julka Jatinder Mohan , Sinha Reshma , Verma Rachna , Kumar Arun , Kumari Shailja , Ameen Fuad , Bhat Sartaj Ahmad , Amarowicz Ryszard , Sharma Mamta
Published: April 2022

Synthetic chemicals, such as fertilizers and pesticides, are abundantly used in agriculture to enhance soil fertility and prevent the occurrence of diseases, respectively. Many studies have reported a negative influence of these chemicals on the soil environment. Natural sources from earthworms and their products, as a result of vermicomposting, may be considered better alternatives. The aim of this review was to reveal the source of antifungal efficiency of vermicompost and its derivatives, such as vermiwash, coelomic fluid, skin secretion of earthworms, and metabolites from decomposer bacteria in vermicompost, in order to highlight their application in agriculture. The synergistic activity of bioactive compounds present in coelomic fluid, mucus, skin secretion, and metabolites from associated bacteria (decomposer) assisted crop plants for effective action against various soil pathogenic fungi, such as Rhizoctoniasolani, Alternaria solani, Aspergillus niger, A.flavus, Fusariumoxysporum, and F. graminearum. Thus, these bioactive metabolites can be recommended to suppress plant fungal diseases. Vermicompost and its derivatives should be considered for use in agricultural fields to control harmful soil fungi and increase crop productivity.
Can a Greenhouse Gas Emissions Tax on Food also Be Healthy and Equitable? A Systemised Review and Modelling Study from Aotearoa New Zealand
Authors: Cleghorn Christine , Mulder Ingrid , Macmillan Alex , Mizdrak Anja , Drew Jonathan , Nghiem Nhung , Blakely Tony , Ni Mhurchu Cliona
Published: April 2022

Policies to mitigate climate change are essential. The objective of this paper was to estimate the impact of greenhouse gas (GHG) food taxes and assess whether such a tax could also have health benefits in Aotearoa NZ. We undertook a systemised review on GHG food taxes to inform four tax scenarios, including one combined with a subsidy. These scenarios were modelled to estimate lifetime impacts on quality-adjusted health years (QALY), health inequities by ethnicity, GHG emissions, health system costs and food costs to the individual. Twenty-eight modelling studies on food tax policies were identified. Taxes resulted in decreased consumption of the targeted foods (e.g., −15.4% in beef/ruminant consumption, N = 12 studies) and an average decrease of 8.3% in GHG emissions (N = 19 studies). The “GHG weighted tax on all foods” scenario had the largest health gains and costs savings (455,800 QALYs and NZD 8.8 billion), followed by the tax—fruit and vegetable subsidy scenario (410,400 QALYs and NZD 6.4 billion). All scenarios were associated with reduced GHG emissions and higher age standardised per capita QALYs for Māori. Applying taxes that target foods with high GHG emissions has the potential to be effective for reducing GHG emissions and to result in co-benefits for population health.
Confirmatory Factor Analysis of Comorbidity between Depression and Aggression in a Child-Adolescent Community Sample: Nosological, Prognosis and Etiological Implications
Authors: Gordillo Rodolfo , Del Barrio Gándara María Victoria , Carrasco Miguel A.
Published: April 2022

Comorbidity between depression and aggression in the child-adolescent population remains a controversial phenomenon. To our knowledge, no longitudinal study using structural equation modeling (SEM) has confirmed whether the relationship between depression and aggression is due to the fact that they share internalizing and externalizing supraordinal factors at the level of the syndrome or is due to the fact that they share common characteristics in relation to an underlying factor at the level of symptoms. We examined longitudinal comorbid relationships in a community sample (N = 251) at three waves ages from 10 to 13 years. The SEM showed that longitudinally, the comorbidity between depression and aggression is due to the fact that they share characteristics of the same underlying factor at the symptom level. These results have implications for the classification, diagnosis, and treatment of comorbidity between depression and aggression in a child-adolescent population.
Enteric Pathogens Risk Factors Associated with Household Drinking Water: A Case Study in Ugu District Kwa-Zulu Natal Province, South Africa
Authors: Khabo-Mmekoa Colette Mmapenya , Genthe Bettina , Momba Maggy Ndombo Benteke
Published: April 2022

The occurrence of diarrheal infections depends on the level of water and sanitation services available to households of immunocompromised individuals and children of less than five years old. It is therefore of paramount importance for immunocompromised individuals to be supplied with safe drinking water for better health outcomes. The current study aimed at ascertaining the probability of infection that Escherichia coli, Salmonella typhimurium, Shigella dysenteriae, Vibrio cholerae, and rotavirus might cause to rural dwellers as compared to urban dwellers. Both culture-based and molecular-based methods were used to confirm the presence of target microorganisms in drinking water samples, while Beta-Poisson and exponential models were used to determine the health risk assessment. Results revealed the presence of all targeted organisms in drinking water. The estimated health risks for single ingestion of water for the test pathogens were as follows: 1.6 × 10−7 for S. typhimurium, 1.79 × 10−4 for S. dysenteriae, 1.03 × 10−3 for V. cholerae, 2.2 × 10−4 for E. coli O157:H7, and 3.73 × 10−2 for rotavirus. The general quantitative risk assessment undertaken in this study suggests that constant monitoring of household container-stored water supplies is vital as it would assist in early detection of microbial pathogens. Moreover, it will also allow the prompt action to be taken for the protection of public health, particularly for immunocompromised individuals and children who are prone to higher risk of infections.
Predictors of Clinical Inertia and Type 2 Diabetes: Assessment of Primary Care Physicians and Their Patients
Authors: Isajev Nemanja , Bjegovic-Mikanovic Vesna , Bukumiric Zoran , Vrhovac David , Lalic Nebojsa M.
Published: April 2022

With the growing prevalence and complex pathophysiology of type 2 diabetes, many patients fail to achieve treatment goals despite guidelines and possibilities for treatment individualization. One of the identified root causes of this failure is clinical inertia. We explored this phenomenon, its possible predictors, and groups of patients affected the most, together with offering potential paths for intervention. Our research was a cross-sectional study conducted during 2021 involving 52 physicians and 543 patients of primary healthcare institutions in Belgrade, Serbia. The research instruments were questionnaires based on similar studies, used to collect information related to the factors that contribute to developing clinical inertia originating in both physicians and patients. In 224 patients (41.3%), clinical inertia was identified in patients with poor overall health condition, long diabetes duration, and comorbidities. Studying the changes made to the treatment, most patients (53%) had their treatment adjustment more than a year ago, with 19.3% of patients changing over the previous six months. Moreover, we found significant inertia in the treatment of patients using modern insulin analogues. Referral to secondary healthcare institutions reduced the emergence of inertia. This assessment of primary care physicians and their patients pointed to the high presence of clinical inertia, with an overall health condition, comorbidities, diabetes duration, current treatment, last treatment change, glycosylated hemoglobin and fasting glucose measuring frequency, BMI, patient referral, diet adjustment, and physician education being significant predictors.
Characteristics, Source Contributions, and Source-Specific Health Risks of PM2.5-Bound Polycyclic Aromatic Hydrocarbons for Senior Citizens during the Heating Season in Tianjin, China
Authors: Zhang Nan , Geng Chunmei , Xu Jia , Zhang Liwen , Li Penghui , Han Jinbao , Gao Shuang , Wang Xinhua , Yang Wen , Bai Zhipeng , Zhang Wenge , Han Bin
Published: April 2022

Polycyclic aromatic hydrocarbons (PAHs) have carcinogenic impacts on human health. However, limited studies are available on the characteristics, sources, and source-specific health risks of PM2.5-bound PAHs based on personal exposure data, and comparisons of the contributions of indoor and outdoor sources are also lacking. We recruited 101 senior citizens in the winter of 2011 for personal PM2.5 sample collection. Fourteen PAHs were analyzed, potential sources were apportioned using positive matrix factorization (PMF), and inhalational carcinogenic risks of each source were estimated. Six emission sources were identified, including coal combustion, gasoline emission, diesel emission, biomass burning, cooking, and environmental tobacco smoking (ETS). The contribution to carcinogenic risk of each source occurred in the following sequence: biomass burning > diesel emission > gasoline emission > ETS > coal combustion > cooking. Moreover, the contributions of biomass burning, diesel emission, ETS, and indoor sources (sum of cooking and ETS) to PAH-induced carcinogenic risk were higher than those to the PAH mass concentration, suggesting severe carcinogenic risk per unit contribution. This study revealed the contribution of indoor and outdoor sources to mass concentration and carcinogenic risk of PM2.5-bound PAHs, which could act as a guide to mitigate the exposure level and risk of PM2.5-bound PAHs.
Responding to Suicide Clusters in the Community: What Do Existing Suicide Cluster Response Frameworks Recommend and How Are They Implemented?
Authors: Hill Nicole T. M. , Robinson Jo
Published: April 2022

Suicide clusters involve an excessive number of suicides, suicide attempts, or both, that occur close in space or time or involve social links between cluster members. Although suicide clusters are rare, evidence documenting the implementation of suicide cluster response activities in communities is required yet remains limited. In this study, we identified the core components of existing suicide cluster response frameworks through a search of the grey literature and conducted an international survey to assess the implementation of the core components by stakeholders with experience responding to a suicide cluster. The following six core components were identified from five cluster response frameworks and were incorporated into a survey assessing stakeholders’ experiences of responding to a suicide cluster: (1) Preparing for a suicide cluster; (2) Routine monitoring of suicide, suicide attempts, and cluster detection; (3) Coordination with the media and monitoring social media; (4) Identifying and supporting individuals at risk; (5) Promoting help-seeking and building community resilience; and (6) Long-term follow-up and evaluation. Twenty-six stakeholders completed the online survey. Many of the core components were implemented by stakeholders. However, gaps in practice were reported in terms of cluster surveillance, monitoring of referral uptake among bereaved individuals, and long-term evaluation. Barriers to implementation included the perceived availability and suitability of mental health services, and availability of long-term funding. Strategic policy and planning that addresses the practice-based experiences of communities has the potential to facilitate a more coordinated and timely response to suicide clusters.
Financial Literacy, Financial Education, and Cancer Screening Behavior: Evidence from Japan
Authors: Nguyen Trinh Xuan Thi , Lal Sumeet , Abdul-Salam Sulemana , Khan Mostafa Saidur Rahim , Kadoya Yoshihiko
Published: April 2022

Although Japan has a well-established cancer screening program and has implemented several initiatives to increase screening rates, levels of cancer screening can be further improved. Based on a rational decision-making framework, this study examines the role of financial literacy and financial education, which measure peoples’ knowledge about investment and savings, respectively, in improving cancer screening rates in Japan. The main data were extracted from Osaka University’s Preference Parameters Study for 2011. The dependent variable was the number of cancer screenings while the two main independent variables were financial literacy and financial education. Ordered probit regression models were run to test the association between financial literacy, financial education, and the number of cancer screenings. The results showed a positive relationship between financial education and cancer screening behavior in Japan, while no significant association was observed between financial literacy and screening behavior. Furthermore, according to findings stratified by three age groups, the positive association between financial education and cancer screening behavior was particularly evident in 50- to 59-year-olds, while the effects of other demographic, socioeconomic, and risky health behavior variables were not consistent. It is imperative that implementation of more financial education programs is an effective intervention to encourage cancer screening behavior in Japanese populations.
Barriers to the Provision of Preventive Care to People Living with Mental Health Conditions: Self-Report by Staff Working in an Australian Community Managed Organisation
Authors: Clinton-McHarg Tara , Gibson Lauren , Bartlem Kate , Murray Sonya , Ryall Jade , Orr Mark , Ford Janet , Bowman Jenny
Published: April 2022

People living with mental health conditions experience a reduced life expectancy largely due to a higher prevalence of chronic diseases. Addressing health risk behaviours, including tobacco smoking, inadequate nutrition, harmful alcohol consumption, and physical inactivity (SNAP), through the provision of preventive care, is recommended to reduce this burden. Community Managed Organisations (CMOs) may play an important role in providing preventive care to consumers with mental health conditions, however, few studies have examined preventive care provision in CMO settings; and no studies have comprehensively assessed barriers to the provision of this care using a tool such as the Theoretical Domains Framework (TDF). To fill this research gap, we conducted an online survey among staff (N = 190) from one CMO in Australia to (1) identify barriers to preventive care provision (ask, advise, assist, connect) to address SNAP behaviours among consumers; and (2) explore associations between barriers and preventive care provision. Results demonstrate that while staff reported knowing how to provide preventive care and believed it would positively impact consumers; barriers including confidence in providing this care and consumer uptake of referrals, were identified. Further research among multiple CMOs is needed to identify care provision and associated barriers in the sector more widely.
Eight Days of L-Citrulline or L-Arginine Supplementation Did Not Improve 200-m and 100-m Swimming Time Trials
Authors: Esen Ozcan , Eser Mustafa Can , Abdioglu Mekki , Benesova Daniela , Gabrys Tomasz , Karayigit Raci
Published: April 2022

The effects of L-citrulline or L-arginine supplementation on exercise performance are equivocal, and the effects on swimming performance are unclear. We aimed to assess whether 8-day supplementation with L-arginine or L-citrulline supplementation would improve 200 m and 100 m freestyle swimming time-trial performances. After the baseline trial (first visit), in a double-blind, randomised design, 15 trained/developmental (5 females) swimmers and triathletes were assigned to three groups and underwent an 8-day supplementation period, with a daily dose of either 8 gr L-arginine (Arg, n = 5) or L-citrulline (Cit, n = 5) or placebo (Pla, n = 5). On day 9, participants completed experimental trial (second visit). In each trial, after blood sampling, participants performed both 200 m and 100 m freestyle swimming time-trials, with 30 min recovery between trials. Plasma nitric oxide (NOx) and blood lactate concentrations (BLa) were collected immediately before and after 200 m and 100 m TTs, respectively. No significant difference was observed in NOx between groups (p = 0.201). There was no significant difference in 200 m (p = 0.226) and 100 m swimming time-trials (p = 0.993) between groups. There was a main effect of time on BLa concentration (p < 0.001), but no trial × group (p = 0.243) and trial × lactate × group interaction effect (p = 0.276) was present. Furthermore, 8-day either L-citrulline or L-arginine supplementation did not enhance middle (200 m) and short-distance (100 m) swimming performance in trained/developmental swimmers and triathletes. These findings do not support the use of L-citrulline or L-arginine supplementation as ergogenic aids for swimming performance.
Effects of a Home-Based Physical Activity Programme on Blood Biomarkers and Health-Related Quality of Life Indices in Saudi Arabian Type-2 Diabetes Mellitus Patients: Protocol for a Randomised Controlled Trial
Authors: Sinclair Jonathan , Ageely Hussein , Mahfouz Mohamed Salih , Hummadi Abdulrahman Ahmed , Darraj Hussain , Solan Yahia , Allan Robert , Bottoms Lindsay
Published: April 2022

The Kingdom of Saudi Arabia is renowned for its high incidence of type-2 diabetes mellitus, with a prevalence rate of around 33%, which is expected to increase to 45.8% by 2030. Engagement in regular physical activity has been shown to significantly attenuate non-communicable diseases including type-2 diabetes. However, the overall rate of physical inactivity among Saudi Arabian adults is currently 80.5%, owing to time pressures, high-density traffic, poor air quality, lack of suitable exercise places/sports facilities, lack of social/friends support, gender, cultural barriers, low self-confidence, lack of time and environmental factors. Previous analyses have shown that home-based activity interventions can be effective. Therefore, given the aforementioned barriers to physical activity in Saudi Arabia; a home-based physical activity may be an ideal solution in type-2 diabetic patients. This manuscript describes the study protocol for a randomized control trial, examining the effects of a home-based physical activity intervention in Saudi Arabian adults with type-2 diabetes. The study will recruit 62 individuals with type-2 diabetes from the Jazan region of the Kingdom of Saudi Arabia, who will be individually randomized to either a physical activity or control group. This 24-week investigation will involve 12-weeks of physical activity in the physical activity group and feature three examination points i.e., baseline, 12-weeks and 24-weeks (follow-up). The primary study outcome is the between-group difference in blood HbA1c levels relative to controls. Secondary outcomes measures will be between-group differences in anthropometric, blood lipid, physical fitness, and patient-reported quality of life outcomes pertinent to type-2 diabetes. Statistical analysis will be conducted on an intention-to-treat basis. The trial has been granted ethical approval by Jazan University, Health Research Ethics Committee (REF: 2177) and formally registered as a trial (NCT04937296). We expect dissemination of the study findings from this investigation to be through publication in a leading peer-reviewed journal.
The Anti-Cancer Potential of Heat-Killed Lactobacillus brevis KU15176 upon AGS Cell Lines through Intrinsic Apoptosis Pathway
Authors: Hwang Chang-Hoon , Lee Na-Kyoung , Paik Hyun-Dong
Published: April 2022

Recent research has focused on the anti-cancer properties of Lactobacillus strains isolated from fermented foods. Their anti-cancer effects are caused by the apoptosis induction in cancer cells. However, sepsis, which can occur when cancer patients consume living organisms, can cause serious conditions in patients with reduced immunity because of cancer. Therefore, this study was conducted using heat-killed Lactobacillus brevis KU15176 (KU15176). To determine the relationship between inflammation and cancer, the anti-inflammatory effect of KU15176 was evaluated using a nitric oxide (NO) assay. Then, 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay was conducted to select cancer cells that showed the anti-proliferative effect of KU15176. Next, reverse transcription-polymerase chain reaction (RT-PCR), 4′,6-diamidino-2-phenylindole (DAPI) staining, flow cytometry, and caspase colorimetric assay were performed. As a result, it was confirmed that KU15176 could cause the increasing expression of apoptosis-related genes (Bax, caspase-3, and caspase-9), DNA breakage, effective apoptosis rate, and increased caspase activity in the human stomach adenocarcinoma (AGS) gastric cancer cell line. In conclusion, these results suggest a potential prophylactic effect of KU15176 against cancer.
In Situ PD-L1 Expression in Oral Squamous Cell Carcinoma Is Induced by Heterogeneous Mechanisms among Patients
Authors: Kondo Yutaro , Suzuki Susumu , Ono Shoya , Goto Mitsuo , Miyabe Satoru , Ogawa Tetsuya , Tsuchida Hiromi , Ito Hideaki , Takahara Taishi , Satou Akira , Tsuzuki Toyonori , Yoshikawa Kazuhiro , Ueda Ryuzo , Nagao Toru
Published: April 2022

The expression of programmed death ligand-1 (PD-L1) is controlled by complex mechanisms. The elucidation of the molecular mechanisms of PD-L1 expression is important for the exploration of new insights into PD-1 blockade therapy. Detailed mechanisms of the in situ expression of PD-L1 in tissues of oral squamous cell carcinomas (OSCCs) have not yet been clarified. We examined the mechanisms of PD-L1 expression focusing on the phosphorylation of downstream molecules of epidermal growth factor (EGF) and interferon gamma (IFN-γ) signaling in vitro and in vivo by immunoblotting and multi-fluorescence immunohistochemistry (MF-IHC), respectively. The in vitro experiments demonstrated that PD-L1 expression in OSCC cell lines is upregulated by EGF via the EGF receptor (EGFR)/PI3K/AKT pathway, the EGFR/STAT1 pathway, and the EGFR/MEK/ERK pathway, and by IFN-γ via the JAK2/STAT1 pathway. MF-IHC demonstrated that STAT1 and EGFR phosphorylation was frequently shown in PD-L1-positive cases and STAT1 phosphorylation was correlated with lymphocyte infiltration and EGFR phosphorylation. Moreover, the phosphorylation pattern of the related molecules in PD-L1-positive cells differed among the cases investigated. These findings indicate that PD-L1 expression mechanisms differ depending on the tissue environment and suggest that the examination of the tissue environment and molecular alterations of cancer cells affecting PD-L1 expression make it necessary for each patient to choose the appropriate combination drugs for PD-1 blockade cancer treatment.