bluuebunny/crossref_metadata_2025 huggingface dataset:
Analysis of Catecholamines and Pterins in Inborn Errors of Monoamine Neurotransmitter Metabolism—From Past to Future
Authors: Jung-Klawitter Sabine , Kuseyri Hübschmann Oya
Published: August 2019

Inborn errors of monoamine neurotransmitter biosynthesis and degradation belong to the rare inborn errors of metabolism. They are caused by monogenic variants in the genes encoding the proteins involved in (1) neurotransmitter biosynthesis (like tyrosine hydroxylase (TH) and aromatic amino acid decarboxylase (AADC)), (2) in tetrahydrobiopterin (BH4) cofactor biosynthesis (GTP cyclohydrolase 1 (GTPCH), 6-pyruvoyl-tetrahydropterin synthase (PTPS), sepiapterin reductase (SPR)) and recycling (pterin-4a-carbinolamine dehydratase (PCD), dihydropteridine reductase (DHPR)), or (3) in co-chaperones (DNAJC12). Clinically, they present early during childhood with a lack of monoamine neurotransmitters, especially dopamine and its products norepinephrine and epinephrine. Classical symptoms include autonomous dysregulations, hypotonia, movement disorders, and developmental delay. Therapy is predominantly based on supplementation of missing cofactors or neurotransmitter precursors. However, diagnosis is difficult and is predominantly based on quantitative detection of neurotransmitters, cofactors, and precursors in cerebrospinal fluid (CSF), urine, and blood. This review aims at summarizing the diverse analytical tools routinely used for diagnosis to determine quantitatively the amounts of neurotransmitters and cofactors in the different types of samples used to identify patients suffering from these rare diseases.
Argentophilic Interactions in Two AgI Complexes of 3-(2-(Pyridin-4-yl)ethyl)pentane-2,4-dione, a Promising Ditopic Ligand
Authors: van Terwingen Steven , Englert Ulli
Published: August 2019

Reactions of 3-(2-(pyridin-4-yl)ethyl)pentane-2,4-dione (HacacPyen) with AgPF6 and AgBF4 afforded cationic silver complexes [Ag(HacacPyen)2]+ with essentially linear coordination of the AgI cation by two pyridine N atoms. Rather unexpectedly, the HacacPyen ligands in the PF6− salt 1 adopt the diketo form, in contrast to the uncoordinated HacacPyen molecule, whereas the corresponding BF4− salt 2 and the majority of 3-substituted acetylacetones crystallizes as the enol tautomer. In both compounds 1 and 2, complex cations aggregate via short Ag···Ag interactions to pairs. These contacts amount to 3.21 Å in 1 and 3.26 Å or 3.31 Å in 2. As they are unsupported by any additional bridging ligands and correspond to the closest interionic interactions between neighbouring complex cations, they may be addressed as argentophilic interactions. The PF6− anions in 1 and the BF4− counter ions in 2 are involved in long and presumably electrostatic Ag···F contacts of ca. 2.9 Å. Additional coordination between AgI and keto O atoms of symmetry-equivalent ligands occurs in 1 and leads to an extended two-periodic supramolecular structure.
Detection of the Flow State for a Centrifugal Pump Based on Vibration
Authors: Lu Jiaxing , Liu Xiaobing , Zeng Yongzhong , Zhu Baoshan , Hu Bo , Yuan Shouqi , Hua Hong
Published: August 2019

A combined numerical and experimental method study was performed to detect the inner flow state for a type of centrifugal pump. It was found that the inlet attack angles of blades in an impeller have a great influence on the flow instabilities in a centrifugal pump. The mechanism of the rotating stall in the impeller channel was explained. Meanwhile, flow state identification with vibration (FSIV) was proposed to detect the flow instabilities in a centrifugal pump. The relationship between the external vibration and the inner flow state has been established by FSIV. The characteristics and mechanism of the vibration produced by the flow instabilities in a centrifugal pump were investigated. It was found that the hump, the rotating stall, the backflow, the occurrence of unstable flow, and the cavitation in the centrifugal pump can be effectively detected by applying the vibration signals, which helps to obtain safe and steady operating conditions for the system.
Two-in-One Fuel Synthetic Bioethanol-Lignin from Lignocellulose with Sewage Sludge and Its Air Pollutants Reduction Effects
Authors: Park Ju-Hyoung , Jin Min-Ho , Lee Young-Joo , Song Gyu-Seob , Choi Jong Won , Lee Dong-Wook , Choi Young-Chan , Park Se-Joon , Song Kwang Ho , Kim Joeng-Geun
Published: August 2019

Developing effective, economical, and environmentally sound approaches for sewage sludge management remains an important global issue. In this paper, we propose a bioethanol-lignin (nonfood biomass)-based sewage sludge upgrading process for enhancing the heating value and reducing air pollutants of hybrid sewage sludge fuel (HSF) for the effective management of sewage sludge. Sewage sludge paste with the lignin-CaO solution implies drying at 105 °C accompanied by torrefaction at 250 °C. During torrefaction, moisture and partly volatile matter begin to evaporate, and are almost vaporized out to the surface. In this study, the proposed process enhances the net caloric value (NCV) to 37%. The lignin-embedded HSF shows a two-in-one combustion peak regardless of the mixing ratio, resulting in a 70% reduction of unburned carbon (UBC) emissions, which is one of the particular matter (PM) sources of combustion flue gas. Other air pollutants, such as CO, hydrocarbon, NOx, and SOx, were also reduced by the proposed process. In particular, SOx emission remained at ~1 ppm (average value) regardless of the sulfur content of the fuel.
Empirical Analysis of Parking Behaviour of Conventional and Electric Vehicles for Parking Modelling: A Case Study of Beijing, China
Authors: Zhuge Chengxiang , Shao Chunfu , Li Xia
Published: August 2019

An empirical study of the parking behaviour of Conventional Vehicles (CVs), Battery Electric Vehicles (BEVs), and Plug-in Hybrid Electric Vehicles (PHEVs) was carried out with the data collected in a paper-based questionnaire survey in Beijing, China. The study investigated the factors that might influence the parking behaviour, with a focus on the maximum acceptable time of walking from parking lot to trip destination, parking fee, the availability of charging posts, the state of charge of EVs and the range anxiety of BEVs. Several Multinomial Logit (MNL) models were developed to explore the relationships between individual attributes and parking choices. The results suggest that (1) the maximum acceptable walking time generally increases with the rise in the amount of saving for parking fee; (2) the availability of charging posts does not influence the maximum acceptable walking time when PHEVs and BEVs have sufficient charge, but the percentage of people willing to walk longer than eight minutes increases from around 35% to 46% when PHEVs are in a low stage of charge; (3) more than half of BEV drivers want the driving range of their vehicles to be one and a half times the driving distance before they depart, given the distance is 50 km. Based on the empirical findings above, a conceptual framework was proposed to explicitly simulate the parking behaviour of both CVs and EVs using agent-based modelling.
Design of Cable Termination for AC Breakdown Voltage Tests
Authors: Andrade Arthur F. , Costa Edson G. , Andrade Filipe L.M. , Soares Clarice S.H. , Lira George R.S.
Published: August 2019

International standards prescribe overvoltage tests to evaluate the insulating material performance of high-voltage cables. However, it is difficult to manage the electric fields at the cable ends when laboratory measurements are carried out because surface and external discharges occur at the cable termination. Therefore, this paper presents a procedure for designing cable terminations to reduce the electric field at the cable ends to appropriate levels even in the case of overvoltage tests. For this purpose, computer simulations of electric field distribution using the finite element method (FEM) were performed. A 35 kV cable model was employed as a sample. An voltage with RMS (root mean square) value of 300 kV was used as an overestimate of breakdown voltage for the internal insulating material. The cable termination model obtained through the proposed methodology allows an electric field reduction in air, preventing the occurrence of external discharges, and thus permitting the breakdown voltage measurement of the cable’s inner insulation.
Greedy Algorithm for Minimizing the Cost of Routing Power on a Digital Microgrid
Authors: Jiang Zhengqi , Sahasrabudhe Vinit , Mohamed Ahmed , Grebel Haim , Rojas-Cessa Roberto
Published: August 2019

In this paper, we propose the greedy smallest-cost-rate path first (GRASP) algorithm to route power from sources to loads in a digital microgrid (DMG). Routing of power from distributed energy resources (DERs) to loads of a DMG comprises matching loads to DERs and the selection of the smallest-cost-rate path from a load to its supplying DERs. In such a microgrid, one DER may supply power to one or many loads, and one or many DERs may supply the power requested by a load. Because the optimal method is NP-hard, GRASP addresses this high complexity by using heuristics to match sources and loads and to select the smallest-cost-rate paths in the DMG. We compare the cost achieved by GRASP and an optimal method based on integer linear programming on different IEEE test feeders and other test networks. The comparison shows the trade-offs between lowering complexity and achieving optimal-cost paths. The results show that the cost incurred by GRASP approaches that of the optimal solution by small margins. In the adopted networks, GRASP trades its lower complexity for up to 18% higher costs than those achieved by the optimal solution.
An Improved Signal Processing Approach Based on Analysis Mode Decomposition and Empirical Mode Decomposition
Authors: Chen Zhongzhe , Liu Baqiao , Yan Xiaogang , Yang Hongquan
Published: August 2019

Empirical mode decomposition (EMD) is a widely used adaptive signal processing method, which has shown some shortcomings in engineering practice, such as sifting stop criteria of intrinsic mode function (IMF), mode mixing and end effect. In this paper, an improved sifting stop criterion based on the valid data segment is proposed, and is compared with the traditional one. Results show that the new sifting stop criterion avoids the influence of end effects and improves the correctness of the EMD. In addition, a novel AEMD method combining the analysis mode decomposition (AMD) and EMD is developed to solve the mode-mixing problem, in which EMD is firstly applied to dispose the original signal, and then AMD is used to decompose these mixed modes. Then, these decomposed modes are reconstituted according to a certain principle. These reconstituted components showed mode mixing phenomena alleviated. Model comparison was conducted between the proposed method with the ensemble empirical mode decomposition (EEMD), which is the mainstream method improved based on EMD. Results indicated that the AEMD and EEMD can effectively restrain the mode mixing, but the AEMD has a shorter execution time than that of EEMD.
Solar Water Heating Systems Applied to High-Rise Buildings—Lessons from Experiences in China
Authors: Huang Junpeng , Fan Jianhua , Furbo Simon , Li Liqun
Published: August 2019

High-rise buildings have a significant impact on the surrounding environment. Building-integrated solar water heating (SWH) systems are effective ways to use renewable energy in buildings. Impediments, such as security concerns, aesthetics and functionality, make it difficult to apply SWH systems in high-rise buildings. At present, only China uses SWH systems on a large scale in such buildings. What are China’s experiences and lessons learned in applying SWH systems in high-rises? Are these experiences scalable to other countries? This study used a combination of field investigation, literature review and case study to summarize 36 systems that had been in operation for 1–14 years. System types, collector types, installation methods, types of auxiliary heat sources, economic performance and various basic principles were summarized. The economic performance of SWH systems in high-rise buildings was analyzed and verified by a case study in Shanghai. The results show that the installation of SWH systems in high-rise buildings is feasible and reliable. Individual household systems (61%) were more popular than centralized systems (25%) and hybrid systems account (14%). The average area of solar collectors per household was 2.17 m2/household, the average design solar fraction was 52%. Flat plate solar collectors (53%) was the most commonly used collector, while electric heating elements (89%) were the most common auxiliary heat sources for SWH systems, followed by gas water heaters and air source heat pumps. The cost of SWH systems per m2 of a building area was between 22 CNY/m2 to 75 CNY/m2. China’s unique practical experience gives a reference for other countries in their efforts to make high-rise buildings more sustainable.
Performance Assessment and Improvement of Central Receivers Used for Solar Thermal Plants
Authors: Albarbar Alhussein , Arar Abdullah
Published: August 2019

In this work, the energy status and supply plans of Saudi Arabia are discussed with a focus on concentrated solar power (CSP) technologies. Subsequently, optimal designs for a 20 MWe solar power plant external receiver, combined with a 15 h thermal energy storage unit, operating under the weather conditions of Neom City, located in northeast Saudi Arabia, is proposed. The effects of receiver tube diameters, tube thicknesses, tube thermal conductivity and receiver’s performance are studied in detail and compared to those used in a well know operational CSP plant. Results show that a smaller tube diameter and thickness give higher receiver thermal efficiency but increase the annual cost of pumping energy. However, that increment in cost is negligible compared to the total energy gained. Furthermore, the aspect ratio is investigated and it was found that a higher aspect ratio gives a higher thermal efficiency. The thermal efficiency of the optimised receiver was increased by about 1% more than the reference plant. In addition, the new design decreases the total estimated cost of tube material by approximately 43%. It is anticipated that the reported results could pave the path for more efficient solar thermal power plants.
How Do Tilia cordata Greenspire Trees Cope with Drought Stress Regarding Their Biomass Allocation and Ecosystem Services?
Authors: Zhang Chi , Stratopoulos Laura Myrtiá Faní , Pretzsch Hans , Rötzer Thomas
Published: August 2019

In the context of climate change, drought is likely to become more frequent and more severe in urban areas. Urban trees are considered to play an important role in fixing carbon, improving air quality, reducing noise and providing other ecosystem services. However, data on the response of urban trees to climate change, particularly to drought, as well as the relationship between their below- and above-ground processes in this context, are still limited, which prevents a comprehensive understanding of the role of urban trees in ameliorating some of the adverse effects of climate change and their ability to cope with it. To investigate whole-plant responses to water shortages, we studied the growth of Tilia cordata Greenspire, a commonly planted urban tree, including development of its roots and stem diameter, leaf parameters and the harvested biomass. Our results showed that this cultivar was susceptible to drought and had reduced biomass in all three compartments: branch (30.7%), stem (16.7%) and coarse roots (45.2%). The decrease in the root:shoot ratio under drought suggested that more carbon was invested in the above-ground biomass. The development of fine roots and the loss of coarse root biomass showed that T. cordata Greenspire prioritised the growth of fine roots within the root system. The CityTree model’s simulation showed that the ability of this cultivar to provide ecosystem services, including cooling and CO2 fixation, was severely reduced. For use in harsh and dry urban environments, we recommend that urban managers take into account the capacity of trees to adapt to drought stress and provide sufficient rooting space, especially vertically, to help trees cope with drought.
Genetic Potential of the Biocontrol Agent Pseudomonas brassicacearum (Formerly P. trivialis) 3Re2-7 Unraveled by Genome Sequencing and Mining, Comparative Genomics and Transcriptomics
Authors: Nelkner Johanna , Torres Tejerizo Gonzalo , Hassa Julia , Lin Timo Wentong , Witte Julian , Verwaaijen Bart , Winkler Anika , Bunk Boyke , Spröer Cathrin , Overmann Jörg , Grosch Rita , Pühler Alfred , Schlüter Andreas
Published: August 2019

The genus Pseudomonas comprises many known plant-associated microbes with plant growth promotion and disease suppression properties. Genome-based studies allow the prediction of the underlying mechanisms using genome mining tools and the analysis of the genes unique for a strain by implementing comparative genomics. Here, we provide the genome sequence of the strain Pseudomonas brassicacearum 3Re2-7, formerly known as P. trivialis and P. reactans, elucidate its revised taxonomic classification, experimentally verify the gene predictions by transcriptome sequencing, describe its genetic biocontrol potential and contextualize it to other known Pseudomonas biocontrol agents. The P. brassicacearum 3Re2-7 genome comprises a circular chromosome with a size of 6,738,544 bp and a GC-content of 60.83%. 6267 genes were annotated, of which 6113 were shown to be transcribed in rich medium and/or in the presence of Rhizoctonia solani. Genome mining identified genes related to biocontrol traits such as secondary metabolite and siderophore biosynthesis, plant growth promotion, inorganic phosphate solubilization, biosynthesis of lipo- and exopolysaccharides, exoproteases, volatiles and detoxification. Core genome analysis revealed, that the 3Re2-7 genome exhibits a high collinearity with the representative genome for the species, P. brassicacearum subsp. brassicacearum NFM421. Comparative genomics allowed the identification of 105 specific genes and revealed gene clusters that might encode specialized biocontrol mechanisms of strain 3Re2-7. Moreover, we captured the transcriptome of P. brassicacearum 3Re2-7, confirming the transcription of the predicted biocontrol-related genes. The gene clusters coding for 2,4-diacetylphloroglucinol (phlABCDEFGH) and hydrogen cyanide (hcnABC) were shown to be highly transcribed. Further genes predicted to encode putative alginate production enzymes, a pyrroloquinoline quinone precursor peptide PqqA and a matrixin family metalloprotease were also found to be highly transcribed. With this study, we provide a basis to further characterize the mechanisms for biocontrol in Pseudomonas species, towards a sustainable and safe application of P. brassicacearum biocontrol agents.
Normalizing Plasma Renin Activity in Experimental Dilated Cardiomyopathy: Effects on Edema, Cachexia, and Survival
Authors: Sullivan Ryan D. , Mehta Radhika M. , Tripathi Ranjana , Gladysheva Inna P. , Reed Guy L.
Published: August 2019

Heart failure (HF) patients frequently have elevated plasma renin activity. We examined the significance of elevated plasma renin activity in a translationally-relevant model of dilated cardiomyopathy (DCM), which replicates the progressive stages (A–D) of human HF. Female mice with DCM and elevated plasma renin activity concentrations were treated with a direct renin inhibitor (aliskiren) in a randomized, blinded fashion beginning at Stage B HF. By comparison to controls, aliskiren treatment normalized pathologically elevated plasma renin activity (p < 0.001) and neprilysin levels (p < 0.001), but did not significantly alter pathological changes in plasma aldosterone, angiotensin II, atrial natriuretic peptide, or corin levels. Aliskiren improved cardiac systolic function (ejection fraction, p < 0.05; cardiac output, p < 0.01) and significantly reduced the longitudinal development of edema (extracellular water, p < 0.0001), retarding the transition from Stage B to Stage C HF. The normalization of elevated plasma renin activity reduced the loss of body fat and lean mass (cachexia/sarcopenia), p < 0.001) and prolonged survival (p < 0.05). In summary, the normalization of plasma renin activity retards the progression of experimental HF by improving cardiac systolic function, reducing the development of systemic edema, cachexia/sarcopenia, and mortality. These data suggest that targeting pathologically elevated plasma renin activity may be beneficial in appropriately selected HF patients.
Ketogenic Diet in Alzheimer’s Disease
Authors: Rusek Marta , Pluta Ryszard , Ułamek-Kozioł Marzena , Czuczwar Stanisław J.
Published: August 2019

At present, the prevalence of Alzheimer’s disease, a devastating neurodegenerative disorder, is increasing. Although the mechanism of the underlying pathology is not fully uncovered, in the last years, there has been significant progress in its understanding. This includes: Progressive deposition of amyloid β-peptides in amyloid plaques and hyperphosphorylated tau protein in intracellular as neurofibrillary tangles; neuronal loss; and impaired glucose metabolism. Due to a lack of effective prevention and treatment strategy, emerging evidence suggests that dietary and metabolic interventions could potentially target these issues. The ketogenic diet is a very high-fat, low-carbohydrate diet, which has a fasting-like effect bringing the body into a state of ketosis. The presence of ketone bodies has a neuroprotective impact on aging brain cells. Moreover, their production may enhance mitochondrial function, reduce the expression of inflammatory and apoptotic mediators. Thus, it has gained interest as a potential therapy for neurodegenerative disorders like Alzheimer’s disease. This review aims to examine the role of the ketogenic diet in Alzheimer’s disease progression and to outline specific aspects of the nutritional profile providing a rationale for the implementation of dietary interventions as a therapeutic strategy for Alzheimer’s disease.
2-Stroke Scavenging in Conventional and Minimally-Modified 4-Stroke Engines for Heavy Duty Applications at Low to Medium Speeds
Authors: Rueter Dirk
Published: August 2019

The transformation of a standard 4-stroke cylinder head into a torque-improved and gradually more efficient 2-stroke design is discussed. The concept with an effective loop scavenging via an extended inlet valve holds promise for engines at low- to medium-rotational speeds for typical designs of conventional 4-stroke cylinder heads. Calculations, flow simulations, and visualizations of experimental flows in relevant geometries and time scales indicate feasibility, followed by a small engine demonstration. Based on presumably long-forgotten and outdated patents, and the central topic of this contribution, an additional jockey rides on the inlet valve’s disk (facing away from the combustion chamber) and reshapes the in-cylinder flow into a reverted tumble. A quick gas exchange with a well-suppressed shortcut into the open exhaust is approached. For overall mechanical efficiency, the required charge pressure for scavenging is of paramount importance due to the short scavenging time and the intake’s reduced cross-section. Herein, still acceptable charging pressures are reported for scavenging periods equivalent to low or medium rotational speeds, as characteristic for heavy-duty applications. Using widely available components (charger, direct injection, variable camshaft angles) an increased engine efficiency is suggested due to the 2-stroke’s downsizing effect (relatively less internal friction as well as the promise of more torque and a decreased size).
Open Versus Robotic Cystectomy: A Propensity Score Matched Analysis Comparing Survival Outcomes
Authors: Moschini Marco , Zamboni Stefania , Soria Francesco , Mathieu Romain , Xylinas Evanguelos , Tan Wei Shen , Kelly John D , Simone Giuseppe , Meraney Anoop , Krishna Suprita , Konety Badrinath , Mattei Agostino , Baumeister Philipp , Mordasini Livio , Montorsi Francesco , Briganti Alberto , Gallina Andrea , Stabile Armando , Sanchez-Salas Rafael , Cathelineau Xavier , Rink Michael , Necchi Andrea , Karakiewicz Pierre I. , Rouprêt Morgan , Koupparis Anthony , Kassouf Wassim , Scherr Douglas S , Ploussard Guillaume , Boorjian Stephen A. , Lotan Yair , Sooriakumaran Prasanna , Shariat Shahrokh F.
Published: August 2019

Background: To assess the differential effect of robotic assisted radical cystectomy (RARC) versus open radical cystectomy (ORC) on survival outcomes in matched analyses performed on a large multicentric cohort. Methods: The study included 9757 patients with urothelial bladder cancer (BCa) treated in a consecutive manner at each of 25 institutions. All patients underwent radical cystectomy with bilateral pelvic lymphadenectomy. To adjust for potential selection bias, propensity score matching 2:1 was performed with two ORC patients matched to one RARC patient. The propensity-matched cohort included 1374 patients. Multivariable competing risk analyses accounting for death of other causes, tested association of surgical technique with recurrence and cancer specific mortality (CSM), before and after propensity score matching. Results: Overall, 767 (7.8%) patients underwent RARC and 8990 (92.2%) ORC. The median follow-up before and after propensity matching was 81 and 102 months, respectively. In the overall population, the 3-year recurrence rates and CSM were 37% vs. 26% and 34% vs. 24% for ORC vs. RARC (all p values > 0.1), respectively. On multivariable Cox regression analyses, RARC and ORC had similar recurrence and CSM rates before and after matching (all p values > 0.1). Conclusions: Patients treated with RARC and ORC have similar survival outcomes. This data is helpful in consulting patients until long term survival outcomes of level one evidence is available.
Combined LDL and VLDL Electronegativity Correlates with Coronary Heart Disease Risk in Asymptomatic Individuals
Authors: Shen Ming-Yi , Hsu Jing-Fang , Chen Fang-Yu , Lu Jonathan , Chang Chia-Ming , Madjid Mohammad , Dean Juliette , Dixon Richard A. F. , Shayani Steven , Chou Tzu-Chieh , Chen Chu-Huang
Published: August 2019

The most electronegative constituents of human plasma LDL (i.e., L5) and VLDL (i.e., V5) are highly atherogenic. We determined whether the combined electronegativity of L5 and V5 (i.e., L5 + V5) plays a role in coronary heart disease (CHD). In 33 asymptomatic individuals (ages 32–64), 10-year hard CHD risk correlated with age (r = 0.42, p = 0.01). However, in age-adjusted analyses, 10-year hard CHD risk correlated with L5 + V5 plasma concentration (r = 0.43, p = 0.01) but not age (p = 0.74). L5 + V5 plasma concentration was significantly greater in the group with high CHD risk (39.4 ± 22.0 mg/dL; n = 17) than in the group with low CHD risk (16.9 ± 14.8 mg/dL; n = 16; p = 0.01). In cultured human aortic endothelial cells, L5 + V5 treatment induced significantly more senescence-associated–β-Gal activity than did equal concentrations of L1 + V1 (n = 4, p < 0.001). To evaluate the in vivo relevance of these findings, we fed ApoE−/− and wild-type mice with a high-fat diet and found that plasma LDL, VLDL, and LDL + VLDL from ApoE−/− mice exhibited significantly greater electrophoretic mobility than did wild-type counterparts (n = 6, p < 0.01). The increased electronegativity of LDL and VLDL in ApoE−/− mice was accompanied by increased aortic lipid accumulation and cellular senescence (n = 6, p < 0.05). Clinical trials are warranted to test the predictive value of L5 + V5 concentration in patients with CHD.
Implementing Eccentric Resistance Training—Part 2: Practical Recommendations
Authors: Suchomel Timothy J. , Wagle John P. , Douglas Jamie , Taber Christopher B. , Harden Mellissa , Haff G. Gregory , Stone Michael H.
Published: August 2019

The purpose of this review is to provide strength and conditioning practitioners with recommendations on how best to implement tempo eccentric training (TEMPO), flywheel inertial training (FIT), accentuated eccentric loading (AEL), and plyometric training (PT) into resistance training programs that seek to improve an athlete’s hypertrophy, strength, and power output. Based on the existing literature, TEMPO may be best implemented with weaker athletes to benefit positional strength and hypertrophy due to the time under tension. FIT may provide an effective hypertrophy, strength, and power stimulus for untrained and weaker individuals; however, stronger individuals may not receive the same eccentric (ECC) overload stimulus. Although AEL may be implemented throughout the training year to benefit hypertrophy, strength, and power output, this strategy is better suited for stronger individuals. When weaker and stronger individuals are exposed to PT, they are exposed to an ECC overload stimulus as a result of increases in the ECC force and ECC rate of force development. In conclusion, when choosing to utilize ECC training methods, the practitioner must integrate these methods into a holistic training program that is designed to improve the athlete’s performance capacity.
Intrahepatic Cholestasis of Pregnancy: A Case Study of the Rare Onset in the First Trimester
Authors: Stulic Milos , Culafic Djordje , Boricic Ivan , Stojkovic Lalosevic Milica , Pejic Nina , Jankovic Goran , Milovanovic Tamara , Culafic-Vojinovic Violeta , Vlaisavljevic Zeljko , Culafic Milica
Published: August 2019

Intrahepatic cholestasis of pregnancy (ICP) is a gestation-specific liver disorder, defined most often as the onset of pruritus, usually from the third trimester of pregnancy, associated with abnormal liver test results and/or increased total serum bile acids and spontaneous relief after delivery. The 21-year-old patient was admitted to our ward in the 11th week of pregnancy due to raised liver enzymes. The first onset of pruritus and jaundice appeared a month before hospitalization. Immunology tests and Toxoplasma gondii were negative. We excluded viral etiology, while alpha-1-antitrypsin, serum and urine copper levels, and thyroid hormones were within the reference values. The patient denied she had taken any medicines and herbal preparations before and during pregnancy. Total bile acids in the serum were significantly elevated (242 μmol/L). The abdominal ultrasound revealed a regular finding. Liver biopsy suggested a cholestatic liver disorder. After a presentation of all risks, the patient decided to stop the pregnancy. After a month, the hepatogram was within the reference values. Very rarely an ICP can occur in early pregnancy (first trimester), which calls for close monitoring. The risk of serious adverse fetal outcomes and spontaneous preterm delivery is proportional with increased levels of maternal serum bile acid.
Metabolomics-Based Studies Assessing Exercise-Induced Alterations of the Human Metabolome: A Systematic Review
Authors: Sakaguchi Camila , Nieman David , Signini Etore , Abreu Raphael , Catai Aparecida
Published: August 2019

This systematic review provides a qualitative appraisal of 24 high-quality metabolomics-based studies published over the past decade exploring exercise-induced alterations of the human metabolome. Of these papers, 63% focused on acute metabolite changes following intense and prolonged exercise. The best studies utilized liquid chromatography mass spectrometry (LC-MS/MS) analytical platforms with large chemical standard libraries and strong, multivariate bioinformatics support. These studies reported large-fold changes in diverse lipid-related metabolites, with more than 100 increasing two-fold or greater within a few hours post-exercise. Metabolite shifts, even after strenuous exercise, typically return to near pre-exercise levels after one day of recovery. Few studies investigated metabolite changes following acute exercise bouts of shorter durations (< 60 min) and workload volumes. Plasma metabolite shifts in these types of studies are modest in comparison. More cross-sectional and exercise training studies are needed to improve scientific understanding of the human system’s response to varying, chronic exercise workloads. The findings derived from this review provide direction for future investigations focused on the body’s metabolome response to exercise.
Germacrone Derivatives as new Insecticidal and Acaricidal Compounds: A Structure-Activity Relationship
Authors: Galisteo Pretel Alberto , Pérez del Pulgar Helena , Guerrero de León Estela , López-Pérez José Luis , Olmeda A. Sonia , Gonzalez-Coloma Azucena , F. Barrero Alejandro , Quílez del Moral José Francisco
Published: August 2019

Currently, the use of synthetic pesticides is the main method of plant protection applied in agri- and horticulture. However, its excessive use leads to the development of pesticide resistance, a contamination of the environment, toxicity to non-target organisms, and risks for human health. With the ultimate aim of contributing to the develop of a more sustainable pest management, we used the natural product germacrone (compound 1), reported to possess significant insecticidal activity, as starting material for the generation of molecular diversity (2–24). Some of the generated derivatives are natural compounds, such as 1,10-epoxygermacrone (2), 4,5-epoxygermacrone (3), gajutsulactone A (7), germacrol (11), isogermacrone (14), 9-hydroxyeudesma-3,7(11)dien-6-one (19), eudesma-4,7(11),dien-8-one (20), eudesma-3,7(11)-dien-8-one (21) and eudesma-4(15),7(11)-dien-8-one (22). Compounds, 7,11-9,10-diepoxigermacr-4,5-en-8-ol (17), 7,11-epoxieudesma-4,7(11)-dien-8-one (23) and 7,11-epoxieudesma-3,7(11)-dien-8-one (24) are described for the first time. The biocidal activity of most of these compounds was assayed against the tick Hyalomma lusitanicum. The acaricidal effects of compound 24 were four times higher than that of germacrone (1). Compound 2 is an insect antifeedant a thousand times more potent than germacrone against Rhopalosiphum padi, which makes this substance a promising selective antifeedant against this cereal pest.
The Inhibitory Effect of (−)-Epigallocatechin-3-Gallate on Breast Cancer Progression via Reducing SCUBE2 Methylation and DNMT Activity
Authors: Sheng Jie , Shi Weilin , Guo Hui , Long Wenlin , Wang Yuxin , Qi Jiangfa , Liu Jinbiao , Xu Yao
Published: August 2019

Epigenetic modifications are important mechanisms responsible for cancer progression. Accumulating data suggest that (−)-epigallocatechin-3-gallate (EGCG), the most abundant catechin of green tea, may hamper carcinogenesis by targeting epigenetic alterations. We found that signal peptide-CUB (complement protein C1r/C1s, Uegf, and Bmp1)-EGF (epidermal growth factor) domain-containing protein 2 (SCUBE2), a tumor suppressor gene, was hypermethylated in breast tumors. However, it is unknown whether EGCG regulates SCUBE2 methylation, and the mechanisms remain undefined. This study was designed to investigate the effect of EGCG on SCUBE2 methylation in breast cancer cells. We reveal that EGCG possesses a significantly inhibitory effect on cell viability in a dose- and time-dependent manner and presents more effects than other catechins. EGCG treatment resulted in enhancement of the SCUBE2 gene, along with elevated E-cadherin and decreased vimentin expression, leading to significant suppression of cell migration and invasion. The inhibitory effect of EGCG on SCUBE2 knock-down cells was remarkably alleviated. Further study demonstrated that EGCG significantly decreased the SCUBE2 methylation status by reducing DNA methyltransferase (DNMT) expression and activity. In summary, this study reported for the first time that SCUBE2 methylation can be reversed by EGCG treatment, finally resulting in the inhibition of breast cancer progression. These results suggest the epigenetic role of EGCG and its potential implication in breast cancer therapy.
Fabrication of Bimetal CuFe2O4 Oxide Redox-Active Nanocatalyst for Oxidation of Pinene to Renewable Aroma Oxygenates
Authors: Mdletshe Lindokuhle S. , Makgwane Peter R. , Ray Suprakas S.
Published: August 2019

This study report on the synthesis of spinel CuFe2O4 nanostructures by surfactant-assisted method. The catalysts were characterized by X-ray diffraction (XRD), laser Raman, transition electron microscope (TEM), scanning electron microscope (SEM), energy dispersive X-ray (EDX), hydrogen temperature programmed reduction (H2-TPR), and Brunauer-Teller-Emmett-Teller (BET) surface area techniques. CuFe2O4 was active for pinene oxidation using tertiary butyl hydroperoxide (TBHP) to pinene oxide, verbenol, and verbenone aroma oxygenates. Under optimized reaction conditions, the spinel CuFe2O4 catalyst could afford 80% pinene conversion at a combined verbenol/verbenone selectivity of 76% within the reaction time of 20 h. The changes in catalyst synthesis solvent composition ratios induced significantly varying redox, phases, and textural structure features, which resulted in various catalytic enhancement effect. Characterization results showed the spinel CuFe2O4 catalyst possessing less than 5 wt% impurity phases, Cu(OH)2, and CuO to afford the best catalytic performance. The CuFe2O4 catalyst was recyclable to up to five reaction cycles without loss of its activity. The recyclability of the bimetal CuFe2O4 oxide catalyst was simply rendered by use of an external magnet to separate it from the liquid solution.
Oxalic Acid-Induced Photodissolution of Ferrihydrite and the Fate of Loaded As(V): Kinetics and Mechanism
Authors: Ren Hai-Tao , Han Jing , Li Ting-Ting , Lin Qi , Lin Jia-Horng , Lou Ching-Wen
Published: August 2019

The fate of arsenic in the water environment is of great concern. Here, the influences of oxalic acid and UV light illumination on the dissolution of naked ferrihydrite (Fhy), Fhy loaded with As(V) [Fhy*-As(V)], as well as the fate of As(V) at pH 3.0 were studied. With the assistance of oxalic acid, complexes of Fe(III)-oxalic acid produced on Fhy/Fhy*-As(V) were reduced to Fe(II)-oxalic acid by photo-induced electrons under UV light irradiation. UV light has nearly no impact on the release of As(V) in the system of Fhy*-As(V) without the assistance of oxalic acid. Nevertheless, in the existence of oxalic acid, UV light illumination resulted in the contents of liberated As(V) decreased by 775–1300% compared to that without light. Considering the coexistence of As(V), oxalic acid as well as iron oxides in aquatic environments, the present study revealed that UV illumination could enhance the retention of As(V) on Fhy in the acidic water environment containing oxalic acid.
Dietary Patterns are Associated with Leukocyte LINE-1 Methylation in Women: A Cross-Sectional Study in Southern Italy
Authors: Barchitta Martina , Maugeri Andrea , Magnano San Lio Roberta , Favara Giuliana , La Rosa Maria Clara , La Mastra Claudia , Quattrocchi Annalisa , Agodi Antonella
Published: August 2019

Bioactive food compounds have different effects on global DNA methylation, an epigenetic mechanism associated with chromosomal stability and genome function. Since the diet is characterized by a mixture of foods, we aimed to identify dietary patterns in women, and to evaluate their association with long interspersed nuclear elements (LINE-1) methylation, a surrogate marker of global DNA methylation. We conducted an observational cross-sectional study of 349 women from Southern Italy, with no history of severe diseases. Dietary patterns were derived by food frequency questionnaire and principal component analysis. LINE-1 methylation of leukocyte DNA was assessed by pyrosequencing. We observed that intake of wholemeal bread, cereals, fish, fruit, raw and cooked vegetables, legumes, soup, potatoes, fries, rice, and pizza positively correlated with LINE-1 methylation levels. By contrast, vegetable oil negatively correlated with LINE-1 methylation levels. Next, we demonstrated that adherence to a prudent dietary pattern—characterized by high intake of potatoes, cooked and raw vegetables, legumes, soup and fish—was positively associated with LINE-1 methylation. In particular, women in the 3rd tertile exhibited higher LINE-1 methylation level than those in the 1st tertile (median = 66.7 %5mC; IQR = 4.67 %5mC vs. median = 63.1 %5mC; IQR = 12.3 %5mC; p < 0.001). Linear regression confirmed that women in the 3rd tertile had higher LINE-1 methylation than those in the 1st tertile (β = 0.022; SE = 0.003; p < 0.001), after adjusting for age, educational level, employment status, smoking status, use of folic acid supplement, total energy intake and body mass index. By contrast, no differences in LINE-1 methylation across tertiles of adherence to the Western dietary pattern were evident. Interestingly, women who exclusively adhered to the prudent dietary pattern had a higher average LINE-1 methylation level than those who exclusively or preferably adhered to the Western dietary pattern (β = 0.030; SE = 0.004; p < 0.001; β = 0.023; SE = 0.004; p < 0.001; respectively), or those with no preference for a specific dietary pattern (β = 0.013; SE = 0.004; p = 0.002). Our study suggested a remarkable link between diet and DNA methylation; however, further mechanistic studies should be encouraged to understand the causal relationship between dietary intake and DNA methylation.
Association of Initial Low Serum Selenium Level with Infectious Complications and 30-Day Mortality in Multiple Trauma Patients
Authors: Choi Soon Bo , Jung Yun Tae , Lee Jae Gil
Published: August 2019

Low serum selenium levels are commonly observed in critically injured multiple trauma patients. This study aimed to identify the association between initial serum selenium levels and in-hospital infectious complications in multiple trauma patients. We retrospectively reviewed multiple trauma patients admitted between January 2015 and November 2017. We selected 135 patients whose serum selenium levels were checked within 48 h of admission. Selenium deficiency was defined as a serum selenium level <70 ng/mL. Survival analyses of selenium deficiency and 30-day mortality were performed. Multivariate logistic regression analysis was performed to identify the association between initial serum selenium level and in-hospital infectious complications. Thirty-day mortality (8.3% vs. 0.0%; p = 0.018) and incidence rates of pneumonia (66.7% vs. 28.3%; p < 0.001) and infectious complications (83.3% vs. 46.5%; p < 0.001) were higher in patients with selenium deficiency than in patients without selenium deficiency. Kaplan–Meier survival cures also showed similar results (log rank test, p = 0.021). Of 135 patients, 76 (56.3%) experienced at least one infectious complication during admission. High injury severity score (ISS, odds ratio (OR) 1.065, 95% confidence interval (CI) 1.024–1.108; p = 0.002) and selenium deficiency (OR 3.995, 95% CI 1.430–11.156; p = 0.008) increased the risk of in-hospital infectious complications in multiple trauma patients. Patients with selenium deficiency showed higher 30-day mortality and higher risks of pneumonia and infectious complications.
Selenoprotein-P Deficiency Predicts Cardiovascular Disease and Death
Authors: Schomburg Lutz , Orho-Melander Marju , Struck Joachim , Bergmann Andreas , Melander Olle
Published: August 2019

Selenoprotein-P (SELENOP) is the main carrier of selenium to target organs and reduces tissue oxidative stress both directly and by delivering selenium to protective selenoproteins. We tested if the plasma concentration of SELENOP predicts cardiovascular morbidity and mortality in the primary preventive setting. SELENOP was measured from the baseline exam in 2002–2006 of the Malmö Preventive Project, a population-based prospective cohort study, using a validated ELISA. Quintiles of SELENOP concentration were related to the risk of all-cause mortality, cardiovascular mortality, and a first cardiovascular event in 4366 subjects during a median (interquartile range) follow-up time of 9.3 (8.3–11) years using Cox proportional Hazards Model adjusting for cardiovascular risk factors. Compared to subjects in the lowest quintile of SELENOP, the risk of all three endpoints was significantly lower in quintiles 2–5. The risk (multivariate adjusted hazard ratio, 95% CI) decreased gradually with the lowest risk in quintile 4 for all-cause mortality (0.57, 0.48–0.69) (p < 0.001), cardiovascular mortality (0.52, 0.37–0.72) (p < 0.001), and first cardiovascular event (0.56, 0.44–0.71) (p < 0.001). The lower risk of a first cardiovascular event in quintiles 2–5 as compared to quintile 1 was significant for both coronary artery disease and stroke. We conclude that the 20% with lowest SELENOP concentrations in a North European population without history of cardiovascular disease have markedly increased risk of cardiovascular morbidity and mortality, and preventive selenium supplementation studies stratified for these subjects are warranted.
The Role of Water Homeostasis in Muscle Function and Frailty: A Review
Authors: Lorenzo Isabel , Serra-Prat Mateu , Yébenes Juan Carlos
Published: August 2019

Water, the main component of the body, is distributed in the extracellular and intracellular compartments. Water exchange between these compartments is mainly governed by osmotic pressure. Extracellular water osmolarity must remain within very narrow limits to be compatible with life. Older adults lose the thirst sensation and the ability to concentrate urine, and this favours increased extracellular osmolarity (hyperosmotic stress). This situation, in turn, leads to cell dehydration, which has severe consequences for the intracellular protein structure and function and, ultimately, results in cell damage. Moreover, the fact that water determines cell volume may act as a metabolic signal, with cell swelling acting as an anabolic signal and cell shrinkage acting as a catabolic signal. Ageing also leads to a progressive loss in muscle mass and strength. Muscle strength is the main determinant of functional capacity, and, in elderly people, depends more on muscle quality than on muscle quantity (or muscle mass). Intracellular water content in lean mass has been related to muscle strength, functional capacity, and frailty risk, and has been proposed as an indicator of muscle quality and cell hydration. This review aims to assess the role of hyperosmotic stress and cell dehydration on muscle function and frailty.
Fast, Spectroscopy-Based Prediction of In Vitro Dissolution Profile of Extended Release Tablets Using Artificial Neural Networks
Authors: Galata Dorián László , Farkas Attila , Könyves Zsófia , Mészáros Lilla Alexandra , Szabó Edina , Csontos István , Pálos Andrea , Marosi György , Nagy Zsombor Kristóf , Nagy Brigitta
Published: August 2019

The pharmaceutical industry has never seen such a vast development in process analytical methods as in the last decade. The application of near-infrared (NIR) and Raman spectroscopy in monitoring production lines has also become widespread. This work aims to utilize the large amount of information collected by these methods by building an artificial neural network (ANN) model that can predict the dissolution profile of the scanned tablets. An extended release formulation containing drotaverine (DR) as a model drug was developed and tablets were produced with 37 different settings, with the variables being the DR content, the hydroxypropyl methylcellulose (HPMC) content and compression force. NIR and Raman spectra of the tablets were recorded in both the transmission and reflection method. The spectra were used to build a partial least squares prediction model for the DR and HPMC content. The ANN model used these predicted values, along with the measured compression force, as input data. It was found that models based on both NIR and Raman spectra were capable of predicting the dissolution profile of the test tablets within the acceptance limit of the f2 difference factor. The performance of these ANN models was compared to PLS models using the same data as input, and the prediction of the ANN models was found to be more accurate. The proposed method accomplishes the prediction of the dissolution profile of extended release tablets using either NIR or Raman spectra.