bluuebunny/crossref_metadata_2025 huggingface dataset:
A Case Study on Integrating a New Key Event Into an Existing Adverse Outcome Pathway on Oxidative DNA Damage: Challenges and Approaches in a Data-Rich Area
Authors: Huliganga Elizabeth , Marchetti Francesco , O’Brien Jason M. , Chauhan Vinita , Yauk Carole L.
Published: April 2022

Adverse outcome pathways (AOPs) synthesize toxicological information to convey and weigh evidence in an accessible format. AOPs are constructed in modules that include key events (KEs) and key event relationships (KERs). This modular structure facilitates AOP expansion and network development. AOP development requires finding relevant information to evaluate the weight of evidence supporting each KER. To do this, the use of transparent/reproducible search methods, such as systematic review (SR), have been proposed. Applying SR to AOP development in a data-rich area is difficult as SR requires screening each article returned from a search. Here we describe a case study to integrate a single new KE into an existing AOP. We explored the use of SR concepts and software to conduct a transparent and documented literature search to identify empirical data supporting the incorporation of a new KE, increase in cellular reactive oxygen species (ROS), upstream of an existing AOP: “Oxidative DNA Damage Leading to Chromosomal Aberrations and Mutations”. Connecting this KE to the AOP is supported by the development of five new KERs, the most important being the first adjacent KER (increase in ROS leading to oxidative DNA damage). We initially searched for evidence of all five KERs and screened 100 papers to develop a preliminary evidence map. After removing papers not containing relevant data based on our Population, Exposure, Comparator and Outcome statement, 39 articles supported one or more KERs; these primarily addressed temporal or dose concordance of the non-adjacent KERs with limited evidence supporting the first adjacent KER. We thus conducted a second focused set of searches using search terms for specific methodologies to measure these first two KEs. After screening, 12 articles were identified that contained quantitative evidence supporting the first adjacent KER. Given that integrating a new KE into an existing AOP requires the development of multiple KERs, this approach of building a preliminary evidence map, focusing evidence gathering on the first adjacent KER, and applying reproducible search strategies using specific methodologies for the first adjacent KER, enabled us to prioritize studies to support expansion of this data-rich AOP.
Detection of Generalized Tonic–Clonic Seizures in Dogs With a Seizure Detection System Established Using Acceleration Data and the Mahalanobis Distance: A Preliminary Study
Authors: Hirashima Junya , Saito Miyoko , Kuriyama Tsukasa , Akamatsu Taketo , Yokomori Minoru
Published: April 2022

Caregivers of dogs with epilepsy experience severe stress due to unpredictable seizures. Hence, they feel the need for a better management strategy. A seizure detection system (SDS), which can identify seizures and provide notifications to caregivers immediately, is required to address this issue. The current study aimed to establish a wearable automatic SDS using acceleration data and the Mahalanobis distance and to preliminarily investigate its feasibility among dogs. A generalized tonic–clonic seizure (GTCS) was targeted because it is the most common type of seizure and can have serious consequences (i.e., status epilepticus). This study comprised three phases. First, the reference datasets of epileptic and non-epileptic activities were established using acceleration data of GTCSs in 3 dogs and daily activities in 27 dogs. Second, the GTCS-detecting algorithm was created using the reference datasets and was validated using other acceleration data of GTCSs in 4 epileptic dogs and daily activities in 27 dogs. Third, a feasibility test of the SDS prototype was performed in three dogs with epilepsy. The algorithm was effective in identifying all acceleration data of GTCSs as seizures and all acceleration data of daily activities as non-seizure activities. Dogs with epilepsy were monitored with the prototype for 48–72 h, and three GTCSs were identified. The prototype detected all GTCSs accurately. A false positive finding was not obtained unless the accelerometer was displaced. Hence, a method that can detect epileptic seizures, particularly GTCSs, was established. Nevertheless, further large-scale studies must be conducted before the method can be commercialized.
A Case Report of Subcutaneously Injected Liposomal Cannabidiol Formulation Used as a Compassion Therapy for Pain Management in a Dog
Authors: Shilo-Benjamini Yael , Cern Ahuva , Zilbersheid Daniel , Hod Atara , Lavy Eran , Barasch Dinorah , Barenholz Yechezkel
Published: April 2022

A 14-year-old intact mixed breed dog (26 kg) was submitted for a novel cannabidiol (CBD) analgesic treatment. The dog was cachectic and had a testicular neoplasia, hip and elbow osteoarthritis and severe cervical pain. Analgesic treatment included canine osteoarthritic supplement, robencoxib and gabapentin. An additional liposomal CBD injectable formulation at 5 mg/kg was administered subcutaneously between the shoulder blades. The dog was monitored using an activity monitoring collar (PetPace), owner wellbeing questionnaire (Canine Brief Pain Inventory; CBPI), pain interactive visual analog scale (iVAS), blood work and CBD plasma concentrations. A week from the injection and up to 3 weeks afterwards the dog had improved CBPI and iVAS pain scores, and increased collar activity scores. CBD was quantified in plasma for 28 days. Due to disease progression, further difficulty to rise and walk, and relapse to pain after 3 weeks, the owners requested a second liposomal CBD injection, which was performed 4 weeks following the first injection using 3 mg/kg dose. Two days later, the dog was found dead in the yard under direct sun, while environmental temperature was 37°C. Major findings on necropsy revealed evidence of heat stroke and severe cervical disc protrusion with spinal hematoma, none related to liposomal CBD. In conclusion, subcutaneous liposomal CBD produced quantifiable CBD plasma concentrations for 28 days and may be an effective additional treatment as part of multimodal pain management in dogs.
Speed Control of a Multi-Motor System Based on Fuzzy Neural Model Reference Method
Authors: I. Breesam Waleed , L. Saleh Ameer , A. Mohamad Khearia , J. Yaqoob Salam , A. Qasim Mohammed , T. Alwan Naseer , Nayyar Anand , Al-Amri Jehad F. , Abouhawwash Mohamed
Published: April 2022

The direct-current (DC) motor has been widely utilized in many industrial applications, such as a multi-motor system, due to its excellent speed control features regardless of its greater maintenance costs. A synchronous regulator is utilized to verify the response of the speed control. The motor speed can be improved utilizing artificial intelligence techniques, for example fuzzy neural networks (FNNs). These networks can be learned and predicted, and they are useful when dealing with nonlinear systems or when severe turbulence occurs. This work aims to design an FNN based on a model reference controller for separately excited DC motor drive systems, which will be applied in a multi-machine system with two DC motors. The MATLAB/Simulink software package has been used to implement the FNMR and investigate the performance of the multi-DC motor. moreover, the online training based on the backpropagation algorithm has been utilized. The obtained results were good for improving the speed response, synchronizing the motors, and applying load during the work of the motors compared to the traditional PI control method. Finally, the multi-motor system that was controlled by the proposed method has been improved where its speed was not affected by the disturbance.
Effect of Catch Crops and Tillage Systems on the Content of Selected Nutrients in Spring Wheat Grain
Authors: Kwiatkowski Cezary Andrzej , Harasim Elżbieta , Klikocka-Wiśniewska Olimpia
Published: April 2022

This paper presents the effects of catch crops (white mustard, lacy phacelia, and a mixture of legumes—faba bean + spring vetch) and two tillage systems (plough tillage and no-tillage) on some quality parameters of spring wheat grain. A field experiment in growing spring wheat in monoculture was conducted in the period 2016–2018 in Czesławice (central part of the Lublin region, Poland). An assumption was made that the nutritional composition of wheat grain could be influenced already at the stage of selection of agronomic practices by modifying the soil chemical and enzyme composition—being the “starting point” for grain quality. It was proven that all the catch crops tested in this study contributed to an improvement in the chemical composition of the soil used in the experiment (a significant increase in humus, P and Mg content). Both the catch crops and the conservation (no-tillage) system stimulated the activity of soil enzymes: dehydrogenase and urease. This resulted in more favorable soil conditions for spring wheat grown in monoculture. The cultivation of the catch crops (particularly white mustard) indirectly contributed to an increased content of dietary fiber and o-dihydroxyphenols in wheat grain. Moreover, the content of most of the amino acids determined and the essential amino acid index (EAAI) in wheat grain were found to be more favorable in the treatments with the catch crops. The highest content of all the macro- and micronutrients analyzed in wheat grain was found in the catch crop treatments (especially that with white mustard). It should be noted that spring wheat responded favorably to the reduced tillage system (no-tillage). Since the grain content of o-dihydroxyphenols, magnesium, calcium, copper, manganese, iron, selenium, and some amino acids (especially essential ones: Lys, Met, Trp) was found to be higher compared to plough tillage.
High-Throughput Virus Screening in Crosses of South American and African Cassava Germplasm Reveals Broad-Spectrum Resistance against Viruses Causing Cassava Brown Streak Disease and Cassava Mosaic Virus Disease
Authors: Sheat Samar , Zhang Xiaofei , Winter Stephan
Published: April 2022

Screening cassava lines for resistance against viruses causing cassava brown streak disease (CBSD) is cumbersome because of the unpredictable and erratic virus infections in the slow plant infection processes that are frequently not associated with distinct leaf symptoms and because of the reliance on the assessment of root necrosis as an indicator of plant resistance/tolerance. The selection of resistant candidates thus extends over several growing cycles and is still associated with uncertainties about the stage of virus infection. To reduce the time for selection of resistant crosses and the uncertainties associated with field screening, we have developed a fast-forward virus screening workflow to assess cassava seedlings from crosses of cassava brown streak- and cassava mosaic virus-resistant parents. After passing through an intensive and precise virus infection routine, cassava seedlings that carried resistance against cassava brown streak and mosaic viruses were identified. Taking the results of 195 seedlings from 18 crossing families together, it became evident that resistance against the viruses causing CBSD is a dominant trait. The protocol developed for virus resistance screening in cassava can be readily adopted. It shifts resistance evaluation from the field to the nursery and replaces the erroneous and lengthy virus infection and screening process with a method of precision and speed.
Macroscopic and Microscopic Phenotyping Using Diverse Yellow Rust Races Increased the Resolution of Seedling and Adult Plant Resistance in Wheat Breeding Lines
Authors: Saleem Kamran , Hovmøller Mogens Støvring , Labouriau Rodrigo , Justesen Annemarie Fejer , Orabi Jihad , Andersen Jeppe Reitan , Sørensen Chris Khadgi
Published: April 2022

We characterized yellow rust (YR) resistance in sixteen winter wheat breeding lines using three different pathogen races and macroscopic and microscopic phenotyping in lab and greenhouse. Three rust races were used on seedlings and two races on fifth and flag leaf growth stages. The wheat lines were previously characterized to possess none or different quantitative trait loci for YR resistance in field trials. At the seedling stage, twelve lines showed race-specific seedling resistance whereas four lines gave strong seedling resistance to all three races. Seven of eight lines with QTL.1B showed strong seedling resistance against the two races also used at fifth and flag leaves. Microscopic phenotyping of line NOS50906215 (QTL.1B) showed small fungal colonies stopped within 3 dpi associated with extensive hypersensitive response (HR). The lines NOS51014910 and NOS51014911 (QTL.3D alone) showed strong adult plant resistance (APR) from the fifth leaf stage. The lines NOS70140801 and NOS70140808 (QTL.3D + 7B) showed strong APR to one race but partial resistance to the other race at all growth stages. Microscopic phenotyping of line NOS70140801 (QTL.3D + 7B) showed more fungal growth and less HR against the race revealing strong APR compared to the one revealing partial resistance. Line NOS51010312 (QTL.7B alone) showed strong APR response against both races whereas line NOS51010313 (QTL.7B) was susceptible. A partial APR response was observed on line NOS51005019 (no QTLs reported). In conclusion, the approach of combining macroscopic and microscopic phenotyping and diverse pathogen races facilitates the identification of multiple and diverse seedling and adult plant resistance responses to yellow rust in wheat.
Potential Use of Tannin Extracts as Additives in Semen Destined for Cryopreservation: A Review
Authors: Liman Mohammed S. , Hassen Abubeker , McGaw Lyndy J. , Sutovsky Peter , Holm Dietmar E.
Published: April 2022

Cryopreservation and storage of semen for artificial insemination (AI) result in excessive accumulation of reactive oxygen species (ROS). This leads to a shortened life span and reduced motility of spermatozoa post-thawing, with consequent impairment of their function. However, certain levels of ROS are essential to facilitate the capacitation of spermatozoa required for successful fertilisation. Tannins, as well-known antioxidant compounds, may act as ROS binders/acceptors/scavengers to inhibit the damaging effects of ROS. This review comprises an analysis of the semen cryopreservation protocol and health functions of tannins, as well as the effects of ROS on fresh and cryopreserved semen’s longevity and fertilisation. Additionally, we surveyed available evidence of the effects of tannin extract feed supplementation on male fertility. We furthermore interrogated existing theories on tannin use as a potential additive to semen extenders, its relationship with semen quality, and to what degree existing theories have been investigated to develop testable new hypotheses. Emphasis was placed on the effects of tannins on ROS, their involvement in regulating sperm structure and function during cryopreservation, and on post-thaw sperm motility, capacitation, and fertilising ability. The diverse effects of tannins on the reproductive system as a result of their potential metal ion chelation, protein precipitation, and biological antioxidant abilities have been identified. The current data are the first to support the further investigation of the incorporation of tannin-rich plant extracts into semen extenders to enhance the post-thaw survival, motility, and fertilising ability of cryopreserved spermatozoa.
Immune Parameters in Chickens Treated with Antibiotics and Probiotics during Early Life
Authors: Jankowski Jan , Tykałowski Bartłomiej , Stępniowska Anna , Konieczka Paweł , Koncicki Andrzej , Matusevičius Paulius , Ognik Katarzyna
Published: April 2022

The aim of the study was to compare the effect of the administration of antibiotics or probiotics on chickens in their first week of life, on selected parameters of humoral and cellular immunity, and on the bursa of Fabricius and spleen indices. The experiment was carried out on 90 one-day-old male broilers. The control group received no additive in the drinking water; the group GP received a probiotic providing Enterococcus faecium and Bacillus amyloliquefaciens; and the group GA received 10% enrofloxacin in the drinking water on the first five days of life. Administration of the antibiotic enrofloxacin or a probiotic containing E. faecium and B. amyloliquefaciens strains to chickens in their first week of life exerts pronounced immunomodulatory effects on humoral and cellular defense mechanisms in these birds. The changes in the subpopulations of B and T cells immediately following early administration of enrofloxacin or the probiotic were not observed at the age of 35 days. Early administration of enrofloxacin can pose a risk of suppression of humoral immunity, as indicated by the significant decrease in the total IgY concentration in the plasma of the chickens.
Obesity Hinders the Protective Effect of Selenite Supplementation on Insulin Signaling
Authors: Hauffe Robert , Rath Michaela , Agyapong Wilson , Jonas Wenke , Vogel Heike , Schulz Tim J. , Schwarz Maria , Kipp Anna P. , Blüher Matthias , Kleinridders André
Published: April 2022

The intake of high-fat diets (HFDs) containing large amounts of saturated long-chain fatty acids leads to obesity, oxidative stress, inflammation, and insulin resistance. The trace element selenium, as a crucial part of antioxidative selenoproteins, can protect against the development of diet-induced insulin resistance in white adipose tissue (WAT) by increasing glutathione peroxidase 3 (GPx3) and insulin receptor (IR) expression. Whether selenite (Se) can attenuate insulin resistance in established lipotoxic and obese conditions is unclear. We confirm that GPX3 mRNA expression in adipose tissue correlates with BMI in humans. Cultivating 3T3-L1 pre-adipocytes in palmitate-containing medium followed by Se treatment attenuates insulin resistance with enhanced GPx3 and IR expression and adipocyte differentiation. However, feeding obese mice a selenium-enriched high-fat diet (SRHFD) only resulted in a modest increase in overall selenoprotein gene expression in WAT in mice with unaltered body weight development, glucose tolerance, and insulin resistance. While Se supplementation improved adipocyte morphology, it did not alter WAT insulin sensitivity. However, mice fed a SRHFD exhibited increased insulin content in the pancreas. Overall, while selenite protects against palmitate-induced insulin resistance in vitro, obesity impedes the effect of selenite on insulin action and adipose tissue metabolism in vivo.
Cisplatin-Induced Reproductive Toxicity and Oxidative Stress: Ameliorative Effect of Kinetin
Authors: Abdel-Latif Rania , Fathy Moustafa , Anwar Hend Ali , Naseem Muhammad , Dandekar Thomas , Othman Eman M.
Published: April 2022

Cisplatin is a commonly used chemotherapeutic agent; however, its potential side effects, including gonadotoxicity and infertility, are a critical problem. Oxidative stress has been implicated in the pathogenesis of cisplatin-induced testicular dysfunction. We investigated whether kinetin use at different concentrations could alleviate gonadal injury associated with cisplatin treatment, with an exploration of the involvement of its antioxidant capacity. Kinetin was administered in different doses of 0.25, 0.5, and 1 mg/kg, alone or along with cisplatin for 10 days. Cisplatin toxicity was induced via a single IP dose of 7 mg/kg on day four. In a dose-dependent manner, concomitant administration of kinetin with cisplatin significantly restored testicular oxidative stress parameters, corrected the distorted sperm quality parameters and histopathological changes, enhanced levels of serum testosterone and testicular StAR protein expression, as well as reduced the up-regulation of testicular TNF-α, IL-1β, Il-6, and caspase-3, caused by cisplatin. It is worth noting that the testicular protective effect of the highest kinetin dose was comparable/more potent and significantly higher than the effects of vitamin C and the lowest kinetin dose, respectively. Overall, these data indicate that kinetin may offer a promising approach for alleviating cisplatin-induced reproductive toxicity and organ damage, via ameliorating oxidative stress and reducing inflammation and apoptosis.
Role of Iron in Aging Related Diseases
Authors: Chen William J. , Kung George P. , Gnana-Prakasam Jaya P.
Published: April 2022

Iron progressively accumulates with age and can be further exacerbated by dietary iron intake, genetic factors, and repeated blood transfusions. While iron plays a vital role in various physiological processes within the human body, its accumulation contributes to cellular aging in several species. In its free form, iron can initiate the formation of free radicals at a cellular level and contribute to systemic disorders. This is most evident in high iron conditions such as hereditary hemochromatosis, when accumulation of iron contributes to the development of arthritis, cirrhosis, or cardiomyopathy. A growing body of research has further identified iron’s contributory effects in neurodegenerative diseases, ocular disorders, cancer, diabetes, endocrine dysfunction, and cardiovascular diseases. Reducing iron levels by repeated phlebotomy, iron chelation, and dietary restriction are the common therapeutic considerations to prevent iron toxicity. Chelators such as deferoxamine, deferiprone, and deferasirox have become the standard of care in managing iron overload conditions with other potential applications in cancer and cardiotoxicity. In certain animal models, drugs with iron chelating ability have been found to promote health and even extend lifespan. As we further explore the role of iron in the aging process, iron chelators will likely play an increasingly important role in our health.
Administration of Glutaredoxin-1 Attenuates Liver Fibrosis Caused by Aging and Non-Alcoholic Steatohepatitis
Authors: Tsukahara Yuko , Ferran Beatriz , Minetti Erika T. , Chong Brian S. H. , Gower Adam C. , Bachschmid Markus M. , Matsui Reiko
Published: April 2022

Liver fibrosis is a sign of non-alcoholic fatty liver disease progression towards steatohepatitis (NASH) and cirrhosis and is accelerated by aging. Glutaredoxin-1 (Glrx) controls redox signaling by reversing protein S-glutathionylation, induced by oxidative stress, and its deletion causes fatty liver in mice. Although Glrx regulates various pathways, including metabolism and apoptosis, the impact of Glrx on liver fibrosis has not been studied. Therefore, we evaluated the role of Glrx in liver fibrosis induced by aging or by a high-fat, high-fructose diet. We found that: (1) upregulation of Glrx expression level inhibits age-induced hepatic apoptosis and liver fibrosis. In vitro studies indicate that Glrx regulates Fas-induced apoptosis in hepatocytes; (2) diet-induced NASH leads to reduced expression of Glrx and higher levels of S-glutathionylated proteins in the liver. In the NASH model, hepatocyte-specific adeno-associated virus-mediated Glrx overexpression (AAV-Hep-Glrx) suppresses fibrosis and apoptosis and improves liver function; (3) AAV-Hep-Glrx significantly inhibits transcription of Zbtb16 and negatively regulates immune pathways in the NASH liver. In conclusion, the upregulation of Glrx is a potential therapeutic for the reversal of NASH progression by attenuating inflammatory and fibrotic processes.
Effect of the First Year of COVID-19 Pandemic on Ophthalmological Practice: A Multi-Centre Italian Study with a Focus on Medico-Legal Aspects
Authors: Demarinis Giuseppe , Mazzuca Daniela , Tatti Filippo , Borselli Massimiliano , Mancini Alessandra , Carnevali Adriano , Logozzo Laura , Veraldi Antonio , Stefano Ottavio , Guarna Francesca , Scorcia Vincenzo , Peiretti Enrico , Giannaccare Giuseppe
Published: April 2022

During the COVID-19 era, several restrictions on surgery have been imposed to reduce the infectious risk among patients and staff and further preserve the availability of critical care resources. The aim of the study was to assess their impact on the ophthalmological practice and its medico-legal implications. A retrospective review of electronic medical records of the ophthalmological departments of the University of Cagliari (SGD) and University Magna Græcia of Catanzaro (UMG), from 16 March 2020 to 14 March 2021 (52 weeks), were compared with data from the corresponding period of the previous year. Weekly data on the number and type of diagnoses and procedures performed were collected and analysed in relation to the weekly average of the total number of COVID-19 patients in intensive care units (ICUs) and inpatients in Sardinia and Calabria. Results showed a significant decrease in cataract surgery operations by 47% and 31%, respectively, in the SGD and UMG (p < 0.05) during the second semester of the year; this drop occurred at the same time as the increase in COVID-19 patients in ICU and those hospitalised in both regions. Additionally, anterior segment surgery decreased at the UMG by 30% (p < 0.05). Vitreoretinal surgery decreased by 27% at the SGD, differently increased amount 31.5% at UMG (p < 0.05). The pandemic had a dramatic impact on elective surgery in ophthalmology, quantifying the backlog is the first step in order to understanding the measures to be taken in near future.
Bio-Inspired Mechano-Sensor Based on the Deformation of Slit Wake
Authors: Wang Kejun , Gao Lei , Gui Yuecheng , Lu Zezhong , Wang Deshan , Li Jiaqiang , Wang Qian
Published: April 2022

Internal mechano-sensors, as an indispensable part of the proprioceptive system of intelligent equipment, have attracted enormous research interest because of their extremely crucial role in monitoring machining processes, real-time diagnosis of equipment faults, adaptive motor control and so on. The mechano-sensory structure with signal-transduction function is an important factor in determining the sensing performance of a mechano-sensor. However, contrary to the wide application of the cantilever beam as the sensory structure of external mechano-sensors in order to guarantee their exteroceptive ability, there is still a lack of an effective and widely used sensory structure to significantly improve the sensing performance of internal mechano-sensors. Here, inspired by the scorpion using the specialized slit as the sensory structure of internal mechano-sensilla, the slit is ingeniously used in the design of the engineered internal mechano-sensor. In order to improve the deformability of the slit wake, the hollowed-out design around the slit tail of biological mechano-sensilla is researched. Meanwhile, to mimic the easily deformed flexible cuticular membrane covering the slit, the ultrathin, flexible, crack-based strain sensor is used as the sensing element to cover the controllable slit wake. Based on the coupling deformation of the slit wake, as well as the flexible strain sensor, the slit-based mechano-sensor shows excellent sensing performance to various mechanical signals such as displacement and vibration signals.
Terahertz Emission Enhanced by a Laser Irradiating on a T-Type Target
Authors: Zhang Ji , Ban Xiaona , Wan Feng , Lv Chong
Published: April 2022

The generation of high field terahertz emission based on the interaction between an ultra-intense laser and solid targets has been widely studied in recent years because of its wide potential applications in biological imaging and material science. Here, a novel scheme is proposed to enhance the terahertz emission, in which a linearly polarized laser pulse irradiates a T-type target including a longitudinal target followed by a transverse target. By using two-dimensional particle-in-cell simulations, we find that the electron beam, modulated by the direct laser acceleration via the interaction of the laser with the longitudinal solid target, plays a crucial role in enhancing the intensity of terahertz emission and controlling its spatial distribution. Compared with the single-layer target, the maximum radiated electromagnetic field’s intensity passing through the spatial probe point is enhanced by about one order of magnitude, corresponding to the terahertz emission power increasing by two orders of magnitude or so. In addition, the proposed scheme is robust with respect to the thickness and length of the target. Such a scheme may provide important theoretical and data support for the enhancement of terahertz emission efficiency based on the ultra-intense laser irradiation of solid targets.
Determination of Zn2+ in Solid Pharmaceutical Dosage Forms by Means of Spectrophotometry in Micellar Media: Method Validation
Authors: Rus Luca Liviu , Juncan Anca Maria , Crăciun Veronica Isabela , Frum Adina , Hegheș Simona-Codruța , Butuca Anca , Dobrea Carmen Maximiliana , Chis Adriana Aurelia , Muntean Andrei Catalin , Vonica-Ţincu Andreea Loredana , Morgovan Claudiu
Published: April 2022

In this study, a spectrophotometric method for the quantitative determination of zinc in a dietary supplement was validated. Zinc forms a ternary complex with Xylenol Orange and cetylpyridinium chloride in acetate buffer at pH = 5.5. The complex exhibits an absorption maximum at 560 nm. Linearity (range was 0.5–1.5 µg/mL, and correlation coefficient of the calibration curve was 0.9998), precision (repeatability variation coefficient and reproducibility variation coefficient were 1.57% and 1.77%, respectively), and accuracy (confidence interval of the mean recovery was 98.29–100.55%) of this method fulfil current pharmacopeial requests. Results obtained by spectrophotometric method were statistically compared with ones obtained by atomic absorption spectrometry and good agreement was found. The method can be applied routinely for quality control of solid pharmaceutical dosage forms containing zinc.
Variable Step Block Hybrid Method for Stiff Chemical Kinetics Problems
Authors: Soomro Hira , Zainuddin Nooraini , Daud Hanita , Sunday Joshua , Jamaludin Noraini , Abdullah Abdullah , Apriyanto Mulono , Kadir Evizal Abdul
Published: April 2022

Integration of a larger stiff system of initial value problems emerging from chemical kinetics models requires a method that is both efficient and accurate, with a large absolute stability region. To determine the solutions of the stiff chemical kinetics ordinary differential equations that help in explaining chemically reactive flows, a numerical integration methodology known as the 3-point variable step block hybrid method has been devised. An appropriate time step is automatically chosen to give accurate results. To check the efficiency of the new method, the numerical integration of a few renowned stiff chemical problems is evaluated such as Belousov–Zhabotinskii reaction and Hires, which are widely used in numerical studies. The results generated are then compared with the MATLAB stiff solver, ode15s.
Review of Offline Payment Function of CBDC Considering Security Requirements
Authors: Chu Yeonouk , Lee Jaeho , Kim Sungjoong , Kim Hyunjoong , Yoon Yongtae , Chung Hyeyoung
Published: April 2022

Due to the growth of the internet and communication technologies, electronic financial systems are becoming popular. Physical cash is losing its preeminence, and digital numbers on computers represent money. However, electronic financial systems, mostly operated by private entities, have defects to be compensated for, such as high charges for using the system, security issues, and the problem of exclusion. As a solution, many countries around the world are considering central bank digital currency. For central bank digital currency to be utilized as a national legal tender, it must be universal and accessible regardless of time and place, similar to physical cash. Therefore, offline payment functions that extend the accessibility of central bank digital currency are becoming attractive. However, due to the characteristics of the electronic financial system, central bank digital currency is vulnerable to possible malicious behaviors in offline situations, such as blackouts and system shutdowns. This paper reviews research studies that deal with security matters related to the offline payment function of central bank digital currency. Offline payment solutions, including central bank digital currency and other electronic financial systems, such as electronic cash and cryptocurrency, are reviewed, and supplemental methods to improve the offline payment solutions of central bank digital currency based on trusted execution environment devices are suggested.
Femtosecond Laser Direct Writing of Gradient Index Fresnel Lens in GeS2-Based Chalcogenide Glass for Imaging Applications
Authors: Delullier Pierre , Druart Guillaume , De La Barrière Florence , Calvez Laurent , Lancry Matthieu
Published: April 2022

Chalcogenide glasses have attracted growing interest for their potential to meet the demands of photonic applications in the Mid-Wavelength InfraRed (MWIR) and Long-Wavelength InfraRed (LWIR) transmission windows. In this work, we investigated the photosensitivity to femtosecond laser irradiation of a dedicated chalcogenide glass, along with its possible applications in micro-optics. In order to address the SWaP problem (Size, Weight and Power), this work took advantage of recent techniques in femtosecond laser direct writing to imprint flat and integrated optical systems. Here, we wanted to simplify an infrared multispectral imaging system which combines a lens array and a filter array. Each channel has a focal length of 7 mm and an f-number of 4. We show in this paper that the chosen GeS2-based chalcogenide glass is very promising for the fabrication of graded index optics by fs-laser writing, and particularly for the fabrication of Fresnel lenses. We note a very important phase variation capacity in this infrared material corresponding to refractive index variations up to +0.055. A prototype of Fresnel GRIN lens with a refractive index gradient was fabricated and optically characterized in the Vis range.
Outcome Prediction for SARS-CoV-2 Patients Using Machine Learning Modeling of Clinical, Radiological, and Radiomic Features Derived from Chest CT Images
Authors: Spagnoli Lorenzo , Morrone Maria Francesca , Giampieri Enrico , Paolani Giulia , Santoro Miriam , Curti Nico , Coppola Francesca , Ciccarese Federica , Vara Giulio , Brandi Nicolò , Golfieri Rita , Bartoletti Michele , Viale Pierluigi , Strigari Lidia
Published: April 2022

(1) Background: Chest Computed Tomography (CT) has been proposed as a non-invasive method for confirming the diagnosis of SARS-CoV-2 patients using radiomic features (RFs) and baseline clinical data. The performance of Machine Learning (ML) methods using RFs derived from semi-automatically segmented lungs in chest CT images was investigated regarding the ability to predict the mortality of SARS-CoV-2 patients. (2) Methods: A total of 179 RFs extracted from 436 chest CT images of SARS-CoV-2 patients, and 8 clinical and 6 radiological variables, were used to train and evaluate three ML methods (Least Absolute Shrinkage and Selection Operator [LASSO] regularized regression, Random Forest Classifier [RFC], and the Fully connected Neural Network [FcNN]) for their ability to predict mortality using the Area Under the Curve (AUC) of Receiver Operator characteristic (ROC) Curves. These three groups of variables were used separately and together as input for constructing and comparing the final performance of ML models. (3) Results: All the ML models using only RFs achieved an informative level regarding predictive ability, outperforming radiological assessment, without however reaching the performance obtained with ML based on clinical variables. The LASSO regularized regression and the FcNN performed equally, both being superior to the RFC. (4) Conclusions: Radiomic features based on semi-automatically segmented CT images and ML approaches can aid in identifying patients with a high risk of mortality, allowing a fast, objective, and generalizable method for improving prognostic assessment by providing a second expert opinion that outperforms human evaluation.
Source Apportionment and Toxic Potency of PM2.5-Bound Polycyclic Aromatic Hydrocarbons (PAHs) at an Island in the Middle of Bohai Sea, China
Authors: Qu Lin , Yang Lin , Zhang Yinghong , Wang Xiaoping , Sun Rong , Li Bo , Lv Xiaoxue , Chen Yuehong , Wang Qin , Tian Chongguo , Ji Ling
Published: April 2022

Polycyclic aromatic hydrocarbons (PAHs) have attracted more attention because of their high atmospheric concentration and toxicity in recent decades. In this study, a total of 60 PM2.5 samples were collected from Beihuangcheng Island in Bohai Sea, China, from August 2017 to March 2018 for analyzing 16 congeners of PAHs (Σ16PAHs). Sources of PAHs were apportioned by a positive matrix factorization (PMF) model and the carcinogenic risk due to exposure to the PAHs was estimated by the toxicity equivalent of BaP (BaPeq). The results showed that the average concentration of Σ16PAHs was 35.3 ± 41.8 ng/m3. The maximum concentration of Σ16PAHs occurred in winter, followed by spring and autumn, and summer. The PMF modeling apportioned the PAHs into four sources, coal combustion, biomass burning, vehicle exhaust, and petroleum release, contributing 43.1%, 25.8%, 24.7%, and 6.39%, respectively. The average ΣBaPeq concentration was 2.32 ± 4.95 ng/m3 during the sampling period, and vehicle exhaust was the largest contributor. The finding indicates that more attention should be paid to reduce the emissions from coal combustion and vehicle exhaust because they were the largest contributors to the PAH concentration in PM2.5 and ΣBaPeq concentration, respectively.
Establishment of an In Vitro Scab Model for Investigating Different Phases of Wound Healing
Authors: Liu Chao , Rinderknecht Helen , Histing Tina , Kolbenschlag Jonas , Nussler Andreas K. , Ehnert Sabrina
Published: April 2022

Chronic wounds are a serious problem in clinical work and a heavy burden for individuals and society. In order to develop novel therapies, adequate model systems for the investigation of wound healing are required. Although in past years different in vitro and in vitro wound healing models have been established, a true human-like model does still not exist. Animal models are limited in their use due to species-specific differences in the skin, a lengthy manufacturing process, experimental costs, and ethical concerns. Both 2D and 3D in vitro models are usually comprised of only one or two skin cell types and fail to capture the reaction between blood cells and skin cells. Thus, our aim was to develop an in vitro scab model to investigate early reactions in the wound healing process. The here established scab model is comprised of HaCaT cells and freshly collected blood from healthy volunteers. The generated scabs were stably cultured for more than 2 weeks. TGF-β signaling is well known to regulate the early phases of wound healing. All three TGF-β isoforms and target genes involved in extracellular matrix composition and degradation were expressed in the in vitro scabs. To validate the in vitro scab model, the effects of either additional stimulation or the inhibition of the TGF-β signaling pathway were investigated. Exogenous application of TGF-β1 stimulated matrix remodeling, which loosened the structure of the in vitro scabs with time, also induced expression of the inhibitory Smad7. Inhibition of the endogenous TGF-β signaling, on the contrary, resulted in a rapid condensation and degranulation of the in vitro scabs. In summary, the here established in vitro scab model can be used to analyze the first phases of wound healing where blood and skin cells interact, as it is viable and responsive for more than 2 weeks.
A Polyhydroxybutyrate (PHB)-Biochar Reactor for the Adsorption and Biodegradation of Trichloroethylene: Design and Startup Phase
Authors: Rossi Marta M. , Alfano Sara , Amanat Neda , Andreini Fabiano , Lorini Laura , Martinelli Andrea , Petrangeli Papini Marco
Published: April 2022

In this work, polyhydroxy butyrate (PHB) and biochar from pine wood (PWB) are used in a mini-pilot scale biological reactor (11.3 L of geometric volume) for trichloroethylene (TCE) removal (80 mgTCE/day and 6 L/day of flow rate). The PHB-biochar reactor was realized with two sequential reactive areas to simulate a multi-reactive permeable barrier. The PHB acts as an electron donor source in the first “fermentative” area. First, the thermogravimetric (TGA) and differential scanning calorimetry (DSC) analyses were performed. The PHB-powder and pellets have different purity (96% and 93% w/w) and thermal properties. These characteristics may affect the biodegradability of the biopolymer. In the second reactive zone, the PWB works as a Dehalococcoides support and adsorption material since its affinity for chlorinated compounds and the positive effect of the “coupled adsorption and biodegradation” process has been already verified. A specific dechlorinating enriched culture has been inoculated in the PWB zone to realize a coupled adsorption and biodegradation process. Organic acids were revealed since the beginning of the test, and during the monitoring period the reductive dichlorination anaerobic pathway was observed in the first zone; no chlorinated compounds were detected in the effluent thanks to the PWB adsorption capacity.
OsOSCA1.1 Mediates Hyperosmolality and Salt Stress Sensing in Oryza sativa
Authors: Han Yang , Wang Yinxing , Zhai Yuanjun , Wen Zhaohong , Liu Jin , Xi Chao , Zhao Heping , Wang Yingdian , Han Shengcheng
Published: April 2022

OSCA (reduced hyperosmolality-induced [Ca2+]i increase) is a family of mechanosensitive calcium-permeable channels that play a role in osmosensing and stomatal immunity in plants. Oryza sativa has 11 OsOSCA genes; some of these were shown to complement hyperosmolality-induced [Ca2+]cyt increases (OICIcyt), salt stress-induced [Ca2+]cyt increases (SICIcyt), and the associated growth phenotype in the Arabidopsis thaliana mutant osca1. However, their biological functions in rice remain unclear. In this paper, we found that OsOSCA1.1 mediates OICIcyt and SICIcyt in rice roots, which are critical for stomatal closure, plant survival, and gene expression in shoots, in response to hyperosmolality and the salt stress treatment of roots. Compared with wild-type (Zhonghua11, ZH11) plants, OICIcyt and SICIcyt were abolished in the roots of 10-day-old ososca1.1 seedlings, in response to treatment with 250 mM of sorbitol and 100 mM of NaCl, respectively. Moreover, hyperosmolality- and salt stress-induced stomatal closure were also disrupted in a 30-day-old ososca1.1 mutant, resulting in lower stomatal resistance and survival rates than that in ZH11. However, overexpression of OsOSCA1.1 in ososca1.1 complemented stomatal movement and survival, in response to hyperosmolality and salt stress. The transcriptomic analysis further revealed the following three types of OsOSCA1.1-regulated genes in the shoots: 2416 sorbitol-responsive, 2349 NaCl-responsive and 1844 common osmotic stress-responsive genes after treated with 250 mM of sorbitol and 125 mM NaCl of in 30-day-old rice roots for 24 h. The Gene Ontology enrichment analysis showed that these OsOSCA1.1-regulated genes were relatively enriched in transcription regulation, hormone response, and phosphorylation terms of the biological processes category, which is consistent with the Cis-regulatory elements ABRE, ARE, MYB and MYC binding motifs that were overrepresented in 2000-bp promoter regions of these OsOSCA1.1-regulated genes. These results indicate that OsOSCA-mediated calcium signaling specifically regulates gene expression, in response to drought and salt stress in rice.
The Actin-Binding Protein Cortactin Promotes Sepsis Severity by Supporting Excessive Neutrophil Infiltration into the Lung
Authors: Lartey Nathaniel L. , Vargas-Robles Hilda , Guerrero-Fonseca Idaira M. , García-Ponce Alexander , Salinas-Lara Citlaltepetl , Rottner Klemens , Schnoor Michael
Published: April 2022

Sepsis is a systemic infection that can lead to multi-organ failure. It is characterised by an uncontrolled immune response with massive neutrophil influx into peripheral organs. Neutrophil extravasation into tissues depends on actin remodeling and actin-binding proteins such as cortactin, which is expressed ubiquitously, except for neutrophils. Endothelial cortactin is necessary for proper regulation of neutrophil transendothelial migration and recruitment to sites of infection. We therefore hypothesised that cortactin plays a crucial role in sepsis development by regulating neutrophil trafficking. Using a murine model of sepsis induced by cecal ligation and puncture (CLP), we showed that cortactin-deficient (KO) mice survive better due to reduced lung injury. Histopathological analysis of lungs from septic KO mice revealed absence of oedema, reduced vascular congestion and mucus deposition, and better-preserved alveoli compared to septic wild-type (WT) mice. Additionally, sepsis-induced cytokine storm, excessive neutrophil infiltration into the lung and oxidative stress were significantly reduced in KO mice. Neutrophil depletion 12 h after sepsis improved survival in WT mice by averting lung injury, similar to both neutrophil-depleted and non-depleted KO mice. Our findings highlight a critical role of cortactin for lung neutrophil infiltration and sepsis severity.
Intein-Mediated Protein Engineering for Cell-Based Biosensors
Authors: Kang Chungwon , Shrestha Keshab Lal , Kwon San , Park Seungil , Kim Jinsik , Kwon Youngeun
Published: April 2022

Cell-based sensors provide a flexible platform for screening biologically active targets and for monitoring their interactions in live cells. Their applicability extends across a vast array of biological research and clinical applications. Particularly, cell-based sensors are becoming a potent tool in drug discovery and cell-signaling studies by allowing function-based screening of targets in biologically relevant environments and enabling the in vivo visualization of cellular signals in real-time with an outstanding spatiotemporal resolution. In this review, we aim to provide a clear view of current cell-based sensor technologies, their limitations, and how the recent improvements were using intein-mediated protein engineering. We first discuss the characteristics of cell-based sensors and present several representative examples with a focus on their design strategies, which differentiate cell-based sensors from in vitro analytical biosensors. We then describe the application of intein-mediated protein engineering technology for cell-based sensor fabrication. Finally, we explain the characteristics of intein-mediated reactions and present examples of how the intein-mediated reactions are used to improve existing methods and develop new approaches in sensor cell fabrication to address the limitations of current technologies.