bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of a Novel Ferroptosis-Related Gene Prognostic Signature in Bladder Cancer
Authors: Sun Jiale , Yue Wenchang , You Jiawei , Wei Xuedong , Huang Yuhua , Ling Zhixin , Hou Jianquan
Published: September 2021

Background

Ferroptosis is a newly found non-apoptotic forms of cell death that plays an important role in tumors. However, the prognostic value of ferroptosis-related genes (FRG) in bladder cancer (BLCA) have not been well examined.

Methods

FRG data and clinical information were collected from The Cancer Genome Atlas (TCGA). Then, significantly different FRGs were investigated by functional enrichment analyses. The prognostic FRG signature was identified by univariate cox regression and least absolute shrinkage and selection operator (LASSO) analysis, which was validated in TCGA cohort and Gene Expression Omnibus (GEO) cohort. Subsequently, the nomogram integrating risk scores and clinical parameters were established and evaluated. Additionally, Gene Set Enrichment Analyses (GSEA) was performed to explore the potential molecular mechanisms underlying our prognostic FRG signature. Finally, the expression of three key FRGs was verified in clinical specimens.

Results

Thirty-two significantly different FRGs were identified from TCGA–BLCA cohort. Enrichment analyses showed that these genes were mainly related to the ferroptosis. Seven genes (TFRC, G6PD, SLC38A1, ZEB1, SCD, SRC, and PRDX6) were then identified to develop a prognostic signature. The Kaplan–Meier analysis confirmed the predictive value of the signature for overall survival (OS) in both TCGA and GEO cohort. A nomogram integrating age and risk scores was established and demonstrated high predictive accuracy, which was validated through calibration curves and receiver operating characteristic (ROC) curve [area under the curve (AUC) = 0.690]. GSEA showed that molecular alteration in the high- or low-risk group was closely associated with ferroptosis. Finally, experimental results confirmed the expression of SCD, SRC, and PRDX6 in BLCA.

Conclusion

Herein, we identified a novel FRG prognostic signature that maybe involved in BLCA. It showed high values in predicting OS, and targeting these FRGs may be an alternative for BLCA treatment. Further experimental studies are warranted to uncover the mechanisms that these FRGs mediate BLCA progression.
The Predictive Value of Clinical and Molecular Characteristics or Immunotherapy in Non-Small Cell Lung Cancer: A Meta-Analysis of Randomized Controlled Trials
Authors: Xu Yangyang , Wang Qin , Xie Jingyuan , Chen Mo , Liu Hongbing , Zhan Ping , Lv Tangfeng , Song Yong
Published: September 2021

Background

This meta-analysis aimed to investigate the efficacy of immune checkpoint inhibitor (ICI)-based therapy in non-small cell lung cancer (NSCLC) patients with different clinical and molecular characteristics such as age, sex, histological type, performance status (PS), smoking status, driver mutations, metastatic site, region and number of prior systemic regimens.

Methods

A systematic literature search was conducted in PubMed, Embase, and the Cochrane library databases to identify qualified randomized controlled trials (RCTs). The primary endpoint was overall survival (OS), and the secondary endpoint was progression-free survival (PFS).

Results

A total of 19 RCTs were included in this meta-analysis. ICI-based therapy significantly improved OS compared with non-ICI therapy in patients aged <65 years (HR, 0.74; P<0.00001), 65-74 years (HR, 0.73; P<0.00001), receiving first-line (HR, 0.75; P<0.00001) or second-line (HR, 0.72; P<0.00001) treatment, current or previous smokers (HR, 0.76; P<0.00001), and EGFR wild-type patients (HR, 0.76; P<0.00001), but not in patients aged ≥75 years (HR, 0.91; P=0.50), receiving third-line treatment (HR, 0.93; P=0.55), never smokers (HR, 0.84; P=0.10), or EGFR mutant patients (HR, 0.99; P=0.92). No statistical OS improvement was observed in KRAS mutant (HR, 0.68; P=0.05) or KRAS wild-type (HR, 0.95; P=0.65) patients. Immunotherapy improved OS in NSCLC patients, regardless of sex (male or female), histological type (squamous or non-squamous NSCLC), PS (0 or 1), metastatic site (brain or liver metastases), and region (East Asia or America/Europe) (all P<0.05). Subgroup analysis showed that the survival benefit of ICIs in patients with brain metastases was observed in first-line combination therapy (P<0.05), but not in second or more line monotherapy (P>0.05). Programmed death-1 (PD-1) inhibitors significantly prolonged OS in patients with liver metastases compared with non-ICI therapy (P=0.0007), but PD-L1 inhibitors did not (P=0.35). Similar results were observed in the combined analysis of PFS.

Conclusions

Age, smoking status, EGFR mutation status, and number of prior systemic regimens predicted the efficacy of immunotherapy. While sex, histological type, PS 0 or 1, KRAS mutation status and region were not associated with the efficacy of ICIs. Patients with liver metastases benefited from anti-PD-1-based therapy, and those with brain metastases benefited from first-line ICI-based combination therapy.

Systematic Review Registration

http://www.crd.york.ac.uk/prospero, identifier CRD42020206062.
Case Report: Rare Presentation of Mixed Germ Cell Tumor in an Infant
Authors: Talluri Sriharsha , Goedde Michael A. , Coventry Susan , Rosenberg Eran , Canalichio Katie L. , Peppas Dennis , White Jeffrey T.
Published: September 2021

The estimated incidence of pediatric testis tumor is 0.5–2.0 per 100,000 children, accounting for 1–2% of all pediatric tumors. Mixed germ cell tumors (MGCT) in prepubertal males are exceedingly rare, with only one previous case report found in the literature. We report a case of a MGCT in an infant. For prepubertal males, GCTs typically present with a painless scrotal mass, though trauma, testis torsion and hydrocele are also common presentations. Similar to such tumors in postpubertal males, ultrasonography, computed tomography, and tumor markers are integral to determine the best treatment. The patient described in this report presented with a painless scrotal mass. Following orchiectomy, the patient was found to have MGCT that was limited to the testis. With prudent management, these patients tend to have favorable prognoses.
Interdisciplinary Physician-Pharmacist Medication Review for Outpatients With Heart Failure: A Subanalysis of the PHARM-CHF Randomized Controlled Trial
Authors: Schumacher Pia M. , Griese-Mammen Nina , Schneider Juliana , Laufs Ulrich , Schulz Martin
Published: September 2021

Aims: Patients with chronic heart failure (CHF) require polypharmacy and are at increased risk for drug-related problems. Interdisciplinary physician-pharmacist medication review may improve drug treatment. Our goal was to analyze the changes from the physician-documented medication plan (MP) and patient-stated medication to an interdisciplinary consolidated MP (CMP).

Methods: This pre-specified subanalysis of the PHARM-CHF randomized controlled trial analyzed the medication review of CHF patients in the pharmacy care group. Community pharmacists compared the MP with the drug regimen stated by the patient and consulted with physicians on identified discrepancies and other medication-related problems resulting in a CMP.

Results: We analyzed 93 patients (mean 74.0 ± 6.6 years, 37.6% female), taking a median of ten (IQR 8–13) drugs. 80.6% of patients had at least one change from MP to CMP. We identified changes in 32.7% (303/926) of drugs. The most common correction was the addition of a drug not documented in the MP to the CMP (43.2%). We also determined frequent modifications in the dosing regimens (37.6%). The omission of a drug documented in the MP but left out of the CMP accounted for 19.1%. Comparing patient-stated medication to CMP, the current drug regimen of patients was changed in 22.4% of drugs.

Conclusion: The medication review resulted in changes of medication between MP and CMP in most of the patients and affected one-third of drugs. Structured physician-pharmacist interdisciplinary care is able to harmonize and optimize the drug treatment of CHF patients.
Genomic Diversity of Pigeon Pea (Cajanus cajan L. Millsp.) Endosymbionts in India and Selection of Potential Strains for Use as Agricultural Inoculants
Authors: Jorrin Beatriz , Maluk Marta , Atoliya Nagvanti , Kumar Shiv Charan , Chalasani Danteswari , Tkacz Andrzej , Singh Prachi , Basu Anirban , Pullabhotla Sarma VSRN , Kumar Murugan , Mohanty Santosh Ranjan , East Alison K. , Ramachandran Vinoy K. , James Euan K. , Podile Appa Rao , Saxena Anil Kumar , Rao DLN , Poole Philip S.
Published: September 2021

Pigeon pea (Cajanus cajan L. Millsp. ) is a legume crop resilient to climate change due to its tolerance to drought. It is grown by millions of resource-poor farmers in semiarid and tropical subregions of Asia and Africa and is a major contributor to their nutritional food security. Pigeon pea is the sixth most important legume in the world, with India contributing more than 70% of the total production and harbouring a wide variety of cultivars. Nevertheless, the low yield of pigeon pea grown under dry land conditions and its yield instability need to be improved. This may be done by enhancing crop nodulation and, hence, biological nitrogen fixation (BNF) by supplying effective symbiotic rhizobia through the application of “elite” inoculants. Therefore, the main aim in this study was the isolation and genomic analysis of effective rhizobial strains potentially adapted to drought conditions. Accordingly, pigeon pea endosymbionts were isolated from different soil types in Southern, Central, and Northern India. After functional characterisation of the isolated strains in terms of their ability to nodulate and promote the growth of pigeon pea, 19 were selected for full genome sequencing, along with eight commercial inoculant strains obtained from the ICRISAT culture collection. The phylogenomic analysis [Average nucleotide identity MUMmer (ANIm)] revealed that the pigeon pea endosymbionts were members of the genera Bradyrhizobium and Ensifer. Based on nodC phylogeny and nod cluster synteny, Bradyrhizobium yuanmingense was revealed as the most common endosymbiont, harbouring nod genes similar to those of Bradyrhizobium cajani and Bradyrhizobium zhanjiangense. This symbiont type (e.g., strain BRP05 from Madhya Pradesh) also outperformed all other strains tested on pigeon pea, with the notable exception of an Ensifer alkalisoli strain from North India (NBAIM29). The results provide the basis for the development of pigeon pea inoculants to increase the yield of this legume through the use of effective nitrogen-fixing rhizobia, tailored for the different agroclimatic regions of India.
Transcriptome and Oxylipin Profiling Joint Analysis Reveals Opposite Roles of 9-Oxylipins and Jasmonic Acid in Maize Resistance to Gibberella Stalk Rot
Authors: Wang Qing , Sun Yali , Wang Fang , Huang Pei-Cheng , Wang Yinying , Ruan Xinsen , Ma Liang , Li Xin , Kolomiets Michael V. , Gao Xiquan
Published: September 2021

Gibberella stalk rot caused by Fusarium graminearum is one of the devastating diseases of maize that causes significant yield losses worldwide. The molecular mechanisms regulating defense against this pathogen remain poorly understood. According to recent studies, a major oxylipin hormone produced by 13-lipoxygenases (LOX) namely jasmonic acid (JA) has been associated with maize susceptibility to GSR. However, the specific roles of numerous 9-LOX-derived oxylipins in defense against Gibberella stalk rot (GSR) remain unexplained. In this study, we have shown that disruption of a 9-LOX gene, ZmLOX5, resulted in increased susceptibility to GSR, indicating its role in defense. To understand how ZmLOX5 regulates GSR resistance, we conducted transcriptome and oxylipin profiling using a zmlox5-3 mutant and near-isogenic wild type B73, upon infection with F. graminearum. The results showed that JA biosynthetic pathway genes were highly up-regulated, whereas multiple 9-LOX pathway genes were down-regulated in the infected zmlox5-3 mutant. Furthermore, oxylipin profiling of the mutant revealed significantly higher contents of several jasmonates but relatively lower levels of 9-oxylipins in zmlox5-3 upon infection. In contrast, B73 and W438, a more resistant inbred line, displayed relatively lower levels of JAs, but a considerable increase of 9-oxylipins. These results suggest antagonistic interaction between 9-oxylipins and JAs, wherein 9-oxylipins contribute to resistance while JAs facilitate susceptibility to F. graminearum.
Gift Game Metatheory: Social Interaction and Interpersonal Growth
Authors: Alonso-Bastarreche Gonzalo , Vargas Alberto I.
Published: September 2021

This paper analyzes Game Theory (GT) from the point of view of moral psychology and makes explicit some of its assumptions regarding the human person as a moral agent, as well as the ends of human action, and reciprocity. Using a largely philosophical methodology, we will argue that GT assumes an instrumental form of rationality underpinned by a logic of self-interest, hence placing individuals, communities, and their social practices in service of external goods and their maximization. Because of this, GT is not adequate to describe the entirety of human social existence and interaction. Nevertheless, by revealing these assumptions, GT can be amplified with another form of rationality based on realist ethics and a personalist anthropology reinforced by the logic of gift. This rationality values the singularity of each person as a holistic unity, as the center of the social realm and as an end in herself called to growth and flourishing with others, nurturing the human community through giving and receiving. We will thus provide a wider philosophical framework for GT with a series of non-mathematical axioms of what can be called a Game Metatheory (GMt). These axioms refer to society as a complex system, not to particular interactions. GMt axioms are not a model of social games, but rather an axiomatic description of social life as a game, revealing its systematic character, complexity, and possible deterioration.
Improving Wellness of Operating Room Personnel: A Light-Based Intervention on Perceived Nursing-Related Stress
Authors: Guerrier Gilles , Margetis Dimitri , Agostini Christine , Machroub Zakia , Di Maria Sophie
Published: September 2021

Background: Nursing is an emotionally demanding and physically draining occupation. Well-being of health care workers is essential to achieve success in care and have good cooperation relationships with other health professionals.

Objective: The purpose of this study was to evaluate the effectiveness of a light-based intervention on perceived nursing-related stress in health care personnel working in an operating room environment.

Methods: A total of 84 nurses participated in this randomized, cross-over controlled study. Intervention consisted of 4 weeks of bright blue-enriched light exposure using a LED head-mounted portable device (n = 42) or no light exposure (n = 42) separated by a 2-week washout period in a crossover fashion. Participants completes questionnaires for the Nursing Stress Scale (NSS).

Results: Intervention and control groups were comparable in terms of demographics, with a median age of 34 (IQR: 27–49) and 69 (82%) female. The mean baseline NSS score was similar in both groups before intervention. The NSS score of the intervention group was significantly lower after intervention than the baseline score: the NSS score difference before and after intervention was 15.1 (SD 7.6) (p < 0.001) and 19.7 (SD 7.5) (p < 0.001) during the two successive periods of intervention, respectively. The cross-group comparison after intervention showed a significantly higher NSS score difference after intervention in the intervention group than the control group: 15.1 (SD 7.6) vs. 1.4 (SD 8.4) (p < 0.001) and 19.7 (SD 7.5) vs. 1.7 (SD 8.9) (p < 0.001) during the two successive periods of intervention, respectively.

Conclusion: Alternative person-directed initiatives should be considered to improve the well-being of the health workforce in operating rooms, especially during the coronavirus pandemic.
Spatial-Temporal Patterns of Air Pollutant Emissions From Landing and Take-Off Cycles in the Yangtze River Delta of China During the COVID-19 Outbreak
Authors: Bao Danwen , Tian Shijia , Zhang Ziqian , Cheng Hao , Zhu Ting , Carpeggiani Nicholas
Published: September 2021

The global aviation industry has been experiencing catastrophic disruption since the beginning of 2020 due to the unprecedented impact of the COVID-19 pandemic on air traffic. Although the decline in regular commercial air travel has caused tremendous economic loss to aviation stakeholders, it has also led to the reduction in the amount of recorded air pollutants. Most of the aircraft emissions are released during the cruise phase of flight, however they have relatively small impact on humans due to the fact that those emissions are released directly into the upper troposphere and lower stratosphere. Therefore, the scope of this study is to investigate the ground-level aircraft emissions from landing and take-off (LTO) cycles, as they have a greater influence on the ambient environment of the airports in a specific region. In this paper, we study the variation of typical air pollutant concentrations (i.e., HC, CO, and NOx) from the LTO cycles during the outbreak of COVID-19 pandemic in both temporal and spatial scales. These ground-level emissions are estimated for the 22 airports in the Yangtze River Delta, China. The results indicate that the variation pattern of the three air pollutants were significantly influenced by the dramatic onset of the COVID-19 pandemic, as well as the pertinent policies to suppress the spread of the virus. The results also reveal non-uniform distribution of the emission quantified at different airports. It is noticeable that the emission quantity generally declined from the east coast to the central and western part of the research region. Furthermore, discrepancies in the target markets also create disparities in the variation pattern of the emissions at different airports under the context of COVID-19.
Prospective Randomized Observational Pilot Trial Evaluating the Effect of Information of Early Childhood Intervention on Stress Levels of Parents of Extremely Low Gestational Age Infants at the NICU
Authors: Resch Bernhard , Fröhlich Judith , Murg Katharina , Pichler-Stachl Elisabeth , Hofbauer-Krug Claudia , Kurz Ronald
Published: September 2021

It is not known to what extent early information on early childhood intervention (ECI) by ECI professionals reduces or increases stress levels of parents having an extremely preterm infant at the neonatal intensive care unit (NICU). Using an observational pilot study, we gave information on ECI in a randomized matter to parents of an extremely low gestational age newborn (ELGAN) at the chronological age of 3–4 weeks (cases) or not (controls). After informed consent, parents judged the infants at the age of 5–7 weeks with the Parental Stressor Scales: Neonatal Intensive Care Unit [PSS: NICU test has three subscales = “Sights and Sounds” (five items), “Parental Role Alteration” (14 items), and “Look and Behave” (seven items)]. Total scales score and subscales scores were comparable between 13 cases and 13 controls over a study period of 1.5 years. Total scores were 9.32 ± 0.72 in the cases compared to 10.02 ± 0.76 in the controls, (95% CI −6.93 to 4.93). Overall, the cases scored lower in most of the items. Early information on ECI at the NICU was provided to parents with an ELGAN did not result in higher stress levels measured with the PSS: NICU. Whether early information on ECI is a strategy, which might be able to reduce parental stress levels, has to be proven in larger studies.
Operationalization of One Health Burnout Prevention and Recovery: Participatory Action Research-Design of Nature-Based Health Promotion Interventions for Employees
Authors: Sterckx Ann , Van den Broeck Kris , Remmen Roy , Dekeirel Kathleen , Hermans Hildegard , Hesters Carmen , Daeseleire Tine , Broes Viki , Barton Jo , Gladwell Valerie , Dandy Sarah , Connors Michéal , Lammel Annamaria , Keune Hans
Published: September 2021

Burnout is, besides a global, complex phenomenon, a public health issue with negative consequences on personal, organizational, social, and economic levels. This paper outlines the co-design of a novel Nature-based Burnout Coaching intervention, called NABUCO. Due to the complexity of burnout, we propose a One Health approach in healthcare, educational and governmental pilot organizations, to deliver guidelines and protocols for prevention and recovery of burnout. We advocate the inclusion of the salutogenic and mutual healing capacity of nature connectedness, facilitating a positive impact on mental and environmental health. A transdisciplinary Participative Action Research-design resulted in an iterative adaptive cycle of co-design, implementation, and evaluation of NABUCO.
Characterizing Cycling Smoothness and Rhythm in Children With and Without Cerebral Palsy
Authors: Sansare Ashwini , Behboodi Ahad , Johnston Therese E. , Bodt Barry , Lee Samuel C. K.
Published: September 2021

Stationary cycling is a practical exercise modality in children with cerebral palsy (CP) that lack the strength for upright exercises. However, there is a lack of robust, sensitive metrics that can quantitatively assess the motor control during cycling. The purpose of this brief report was to characterize the differences in motor control of cycling in children with CP and with typical development by developing novel metrics to quantify cycling smoothness and rhythm. Thirty one children with spastic diplegic CP and 10 children with typical development cycled on a stationary cycle. Cycling smoothness was measured by cross-correlating the crank angle with an ideal cycling pattern generated from participant-specific cadence and cycling duration. Cycling rhythmicity was assessed by evaluating the revolution-to-revolution variability in the time required to complete a revolution. Statistically significant differences (p < 0.001) using the Wilcoxon Rank Sum test were found between the two groups for both the metrics. Additionally, decision tree analysis revealed thresholds of smoothness <0.01 and rhythm <0.089–0.115 s for discriminating a less smooth, irregular cycling pattern characteristic of CP from typical cycling. In summary, the objective measures developed in this study indicate significantly less smoothness and rhythm of cycling in children with CP compared to children with typical development, suggestive of altered coordination and poor motor control. Such quantitative assessments of cycling motion in children with CP provide insights into neuromotor deficits that prevent them from cycling at intensities required for aerobic benefits and for participating in cycling related physical activities with their peers.
Computer Simulations in Service of Biology
Authors: Levitt Michael
Published: September 2021

Computer simulation is an important research tool in today’s scientific world. Computers allow us to perform computations that mimic the behavior of complex (biological) systems in ways that we could not otherwise achieve. You could think of these simulations as a computer game, in which a virtual world is created that works according to certain (e.g., physical) rules. While we play the game, we learn the rules governing this virtual world and its environment, and also the way that we affect this world as players. In this article, I will explain how we use computer simulations in the world of structural biology to study the structure and function of molecules. I will also describe how I think that we could use insights from the world of biology and computer simulations to advance the society that we live in.
The Central Valley Salmonid Story: Six Million Years in the Making
Authors: Glenn Hilary , Fejtek Stacie , Rennert Jacob
Published: September 2021

For over six million years, salmon and steelhead (known as salmonids) have returned to the California Central Valley. After swimming under the Golden Gate Bridge and through the San Francisco Bay, adult salmonids swim hundreds of miles up the Sacramento or San Joaquin Rivers. Before dams were built, three out of the five salmonid species swam from the Central Valley into high-elevation, cold-water streams within the Sierra Nevada, southern Cascades, and Coastal Mountain ranges to finish their life cycles. Unfortunately, barriers such as dams cut-off salmonids from their home streams. When salmonid populations decrease, the effects are felt across the ecosystem—everything from microbes to humans feels the disconnection between the rivers and the ocean. Governmental agencies and their partners are teaming up to restore Central Valley salmonid populations by reconnecting them to their habitats and putting them back into high-elevation streams.
Targeted Degradation of Proteins — The Ubiquitin System
Authors: Ciechanover Aaron
Published: September 2021

Proteins are the engines of all forms of life, for humans and for all the plant and animal kingdoms. Proteins are used both to build organs (such as bones, muscles, and skin) and to perform bodily functions. These functions range from digestion (processing food and converting it into energy), to enabling movement and sensation (sight and hearing), to protecting the body from foreign invaders with our antibodies, which are also proteins. What are proteins? They can be compared to words in a language that contains letters. In the Hebrew alphabet, there are 26 letters out of which countless words can be composed. But when we write, we use just a fraction of these infinite options, with the average number of letters in a word ranging between 3 and 8. The biological “protein alphabet” is comprised of 20 “letters” called amino acids, which are the building blocks of the proteins that make up the body. Proteins are chains of amino acid, linked together in a specific order governed by the DNA. Unlike the words of a spoken language, the average protein consists of hundreds of amino acids. The extensive length of proteins and the chemical composition of the amino acids make proteins sensitive to many factors, such as high temperatures, radiation, and chemicals. All these factors damage proteins and alter their fragile structures, negatively affecting how they function. When proteins are damaged or when they finish performing their functions and are no longer needed, the body breaks them down. With my doctoral adviser, Prof. Avram Hershko, and our research collaborator, Prof. Irwin Rose from the Fox Chase Cancer Center in Philadelphia, we discovered the mechanism responsible for targeted degradation of proteins in cells. This degradation can recognize damaged proteins or proteins that are not needed anymore, while leaving intact the “healthy,” functional ones. This mechanism is called the ubiquitin system after its principal protein, ubiquitin, which was the first protein we discovered in the system. Ubiquitin’s role is to tag undesirable proteins so that the cell’s “grinder” can recognize them and break them down, enabling the cell to function normally. In this article, we will explain the story of proteins and the ubiquitin system that we discovered in a study that earned us, among other prizes, the Nobel Prize in Chemistry in 2004.
Fracture Resistance of Equine Cheek Teeth With and Without Occlusal Fissures: A Standardized ex vivo Model
Authors: Pollaris Elke , Broeckx Bart J. G. , Rajasekharan Sivaprakash , Cauwels Rita , Vlaminck Lieven
Published: September 2021

Background:Ex vivo fracture models are frequently used in human dentistry to provide insights in the fracture mechanisms of teeth. Equine cheek teeth fractures are an important dental pathology, but there has been no research performed to examine the fracture resistance ex vivo.

Objective: To evaluate the fracture resistance of equine cheek teeth and identify anatomical predictors that might influence fracture resistance in healthy teeth. It was further evaluated if the presence of a fissure caused a decrease in fracture resistance.

Study design:Ex vivo experimental design.

Methods: Individual cheek teeth were subjected to a compression load in a universal testing machine until fracture occurred. Testing was performed in two study groups. A first group of healthy cheek teeth was tested to examine anatomical predictors on fracture resistance. A second group comprised cheek teeth with occlusal fissures and an equal number of age- and size-matched fissure-free teeth as controls. The effect of possible predictors on fracture resistance was investigated by regression analysis.

Results: In the first group, fracture resistance was significantly influenced by the location on the tooth where testing was performed in both maxillary (p < 0.001) and mandibular teeth (p < 0.001). Additional significantly associated factors were Triadan number in mandibular (p = 0.009) and the mesiodistal length of the occlusal surface of maxillary teeth (p = 0.01). Experimentally induced crown fractures that extended below the simulated bone level were more frequently associated with pulp horn exposure (p < 0.001). In the second group, significant lower fracture loads were recorded in teeth with fissures (mandibular p = 0.006; maxillary p < 0.001), compared to fissure-free teeth.

Main limitations: This ex vivo model does not imitate the in vivo masticatory forces and lacks the shock-absorbing properties of the periodontal ligament.

Conclusions: The methodology used in this study provides an ex vivo experimental set-up to test fracture resistance of equine cheek teeth enabling evidence-based research to examine the potentially weakening effects of tooth pathology and its treatments. Crown resistance to fracture differed along the occlusal surface of healthy equine cheek teeth, and the presence of fissures further decreased fracture resistance.
Industrial and Bioenergy Crops for Bioeconomy Development
Authors: Stolarski Mariusz Jerzy
Published: September 2021

The production of industrial and bioenergy crops has been the subject of scientific research for many years; however, the implementation of previously proposed solutions for commercial production is still at an early stage [...]
Neofusicoccum batangarum Causing Dieback of Mango (Mangifera indica) in Florida
Authors: Puig Alina S. , Winterstein Mike C.
Published: September 2021

Mango (Mangifera indica) is an economically significant crop, and is affected by dieback in nearly all commercial production areas. Due to the wide range of organisms previously associated with these disease symptoms in Florida, isolations and pathogenicity tests were carried out to determine the causal organism. The pathogen was identified as Neofusicoccum batangarum based on genetic sequences from three loci (internal transcribed spacer of the rDNA (ITS), β-tubulin (BT), and translation elongation factor 1-α (EF)), recommended for members of the Botryosphaeriaceae family. Possible infection routes were determined by inoculating wounded and unwounded stems with N. batangarum. Trees wounded prior to pathogen inoculation developed larger lesions (5.85 cm ± 1.51) than unwounded trees (0.51 cm ± 0.48), p < 0.0003. In addition, lesions only developed at a small number of inoculation sites in the absence of wounds (14.3%), compared to 93% when stems were wounded. No necrosis was observed in the negative controls. This study provides molecular data on N. batangarum, and evidence of its role causing mango dieback in Florida.
Dynamics of Clomazone Formulations Combined with Sulfentrazone in Sugarcane (Saccharum spp.) Straw
Authors: Tropaldi Leandro , Carbonari Caio A. , de Brito Ivana Paula F. S. , de Matos Ana Karollyna A. , de Moraes Carolina P. , Velini Edivaldo D.
Published: September 2021

Herbicide formulations can alter the herbicide performance, affecting the application safety and weed control efficiency. Thus, the objective of this work was to compare the dynamics of clomazone herbicide applied single and combined with sulfentrazone on sugarcane (Saccharum spp.) straw. Laminated polypropylene containers filled with sugarcane straw (10 t ha−1) were subjected to two clomazone formulations (microencapsulated and conventional formulations; 1200 g ha−1) applied single or combined with sulfentrazone (600 g ha−1) with four replications, and the experiment was duplicated. The application was performed indoors with an automated sprayer. After application, accumulated rainfall depths (0, 5, 10, 20, 50, and 100 mm) on the treated containers were simulated soon after the herbicide applications, and the percolated waters were subsequently collected for herbicide quantification by chromatography and mass spectrometry (LC-MS/MS). The microencapsulated formulation of clomazone applied single or combined with sulfentrazone enabled the recovery of higher quantity of clomazone (>80%), with the advantage that a large percentage remained encapsulated (>70%), thus decreasing losses and increasing the product efficiency. The 30 mm simulated rainfall efficiently carried the clomazone herbicide when its microencapsulated formulation was applied, whereas its conventional formulation required higher rainfall depths (60 mm). Sulfentrazone was easily carried through the sugarcane straw by the rainfall depths when it was combined with clomazone, regardless of the clomazone formulation. The clomazone formulation affect the percolation dynamics of this herbicide through the sugarcane straw.
Effect of Forage Plant Mixture and Biostimulants Application on the Yield, Changes of Botanical Composition, and Microbiological Soil Activity
Authors: Zielewicz Waldemar , Swędrzyński Arkadiusz , Dobrzyński Jakub , Swędrzyńska Dorota , Kulkova Iryna , Wierzchowski Paweł Stanisław , Wróbel Barbara
Published: September 2021

Recently, an increasing interest in such fertilizers and fertilization methods which not only directly supply nutrients to plants, but also stimulate soil bioactivity is noted. Their effect on both soil microbiota and forage plants has not been fully recognized. The aim of the study was to investigate the combined effect of forage plant mixture type and mineral fertilizers (NPK) with biostimulants based on a marine algae extracts on the botanical composition, yield, the structure of selected taxonomic and trophic groups of soil microorganisms, and the soil enzymatic activity. During the years 2018–2019 a field experiment established in split-plot design with two different forage plant mixtures, as a first factor, and different fertilization basing on mineral fertilizers amended with biostimulants, as a second factor was conducted. Two types of forage mixtures of sown species were used: grass mixture (GM) and legume-grass mixture (LGM). Every year the following biostimulants were applied: N-14, PinKstart, Physiostart, Physioactive and they were compared with standard NPK fertilisation and no fertilisation as a control. The reaction of forage plant mixtures on applied fertilisation was different. The intensive development of grass species, mainly Lolium perenne, at the expense of Trifolium repens share in LGM was observed. In GM sward dominated Dactylis glomerata. A beneficial effects of biostimulants’ application on the biomass yields of both grass mixtures was observed. The systematic soil acidification and a decrease of soil enzymatic activity in result of applied fertilization, except NPK + Physioactive treatment (calcium fertilizer containing 76% calcium carbonate), was noted. Soil reaction to applied fertilisation was dependent on the botanical composition of the sward. The counts of microorganisms in the soil under LGM were almost two times higher than in the soil under GM. The most effective, in reducing the negative effect of nitrogen mineral fertilization on the pH of soil, was fertilization with NPK + Physioactiv.
Assessment of Soft Computing Techniques for the Prediction of Suspended Sediment Loads in Rivers
Authors: Khan Muhammad Adnan , Stamm Jürgen , Haider Sajjad
Published: September 2021

A key goal of sediment management is the quantification of suspended sediment load (SSL) in rivers. This research focused on a comparison of different means of suspended sediment estimation in rivers. This includes sediment rating curves (SRC) and soft computing techniques, i.e., local linear regression (LLR), artificial neural networks (ANN) and the wavelet-cum-ANN (WANN) method. Then, different techniques were applied to predict daily SSL at the Pirna and Magdeburg Stations of the Elbe River in Germany. By comparing the results of all the best models, it can be concluded that the soft computing techniques (LLR, ANN and WANN) better predicted the SSL than the SRC method. This is due to the fact that the former employed non-linear techniques for the data series reconstruction. The WANN models were the overall best performer. The WANN models in the testing phase showed a mean R2 of 0.92 and a PBIAS of −0.59%. Additionally, they were able to capture the suspended sediment peaks with greater accuracy. They were more successful as they captured the dynamic features of the non-linear and time-variant suspended sediment load, while other methods used simple raw data. Thus, WANN models could be an efficient technique to simulate the SSL time series because they extract key features embedded in the SSL signal.
Squashed-Slice Algorithm Based on STEP-NC for Multi-Material and Multi-Directional Additive Processes
Authors: Um Jumyung , Park Joungmin , Stroud Ian Anthony
Published: September 2021

Even though additive manufacturing is receiving increasing interest from aerospace, automotive, and shipbuilding, the legacy approach using tessellated form representation and cross-section slice algorithm still has the essential limitation of its inaccuracy of geometrical information and volumetric losses of final outputs. This paper introduces an innovative method to represent multi-material and multi-directional layers defined in boundary-representation standard model and to process complex sliced layers without missing volumes by using the proposed squashing operation. Applications of the proposed method to a bending part, an internal structure, and an industrial moulding product show the assurance of building original shape without missing volume during the comparison with the legacy method. The results show that using boundary representation and te squashing algorithm in the geometric process of additive manufacturing is expected to improve the inaccuracy that was the barrier of applying additive process to various metal industries.
Characteristics and Valorization Potential of Fermentation Waste of Greengage (Prunus mume)
Authors: Wang Jing , Wang Jingluan , Wu Chongde , Huang Jun , Zhou Rongqing , Jin Yao
Published: September 2021

Greengage wine is gaining increasing attention in Asia for its rich nutritional elements and medicinal value. However, the treatment of the fermentation waste after brewing is a problem that remains unsolved. This work proposed to valorize the fermentation waste extract by a simple centrifugation. The bioactive compounds of the fermentation waste were investigated, including total flavonoids, total phenols, specific phenols and volatile compounds. The antioxidant and antibacterial capacities of the waste extract were also evaluated. The results revealed that the total phenol (1.34 mg GAE/g EPW) and total flavonoid (1.17 mg RE/g EPW) of the fermentation waste extract were still considerable. The fermentation waste also showed high DPPH radical scavenging capacity (5.39 μmol TE/g EPW) and high ABTS radical scavenging capacity (9.80 μmol TE/g EPW). Both GC-MS and LC-MS analysis identified key bioactive compounds, such as linalool, terpineol, β-ionone, neochlorogenic acid and chlorogenic acid, which have high antioxidant capacity and strong, thermal-stable antibacterial capacity. All these characteristics show a promising future for valorized fermentation waste, for example, in food additives or mouthwash.
Effect of Soy Protein Products and Gum Inclusion in Feed on Fecal Particle Size Profile of Rainbow Trout
Authors: Welker Thomas L. , Liu Keshun , Overturf Ken , Abernathy Jason , Barrows Frederic T.
Published: September 2021

Replacement of fishmeal (FM) with alternative plant proteins, especially soybean meal (SBM), can cause a diarrhea-like symptom in rainbow trout (RBT), characterized by very fine fecal particles. These fines do not settle out in raceway effluent for collection and can contribute to pollution of receiving waters. In this study, two experiments were conducted. Experiment 1 examined effects of nine protein sources (sardine meal, menhaden meal, soy protein concentrates (SPC) (three types), SBM (regular and high protein), corn protein concentrate (CPC), and poultry by-product meal (PBM)) on fecal particle size distribution. Results showed that all five soy-based diets produced feces in RBT having 75.7–89.3% fines and only about 1.0% large particles, while the remaining four diets yielded feces having a balanced particle size distribution. Oligosaccharides present naturally in soy products, thought to contribute fecal fines, were not correlated to fecal particle size classes. Instead, high crude fiber content in soy-based diets was found to be responsible for unbalanced fecal particle distribution in RBT. Experiment 2 examined if improvements in formulation could reduce the negative effect of soy-based ingredients. Eight practical diets (FM, SPC, SPC + 0.3% guar gum, PBM + CPC, PBM + CPC + 20 or 30% SPC, and PBM + CPC + 20 or 30% SPC + 0.3% guar gum) were formulated to contain 40% protein and 20% lipid. Results showed that diets containing mixtures of PBM, CPC, and 20% or 30% SPC plus guar gum produced trout feces with the highest percentage of large particles and lowest of fines, while the diet containing SPC alone (56%) plus guar gum resulted in trout feces having the highest content of mid-size particles. It was concluded that crude fiber in soy protein products (SBM and SPC) caused undesirable fecal particle profiles in RBT, and the addition of guar gum could significantly alleviate this negative effect.
Image Encryption and Decryption System through a Hybrid Approach Using the Jigsaw Transform and Langton’s Ant Applied to Retinal Fundus Images
Authors: Romero-Arellano Andrés , Moya-Albor Ernesto , Brieva Jorge , Cruz-Aceves Ivan , Avina-Cervantes Juan Gabriel , Hernandez-Gonzalez Martha Alicia , Lopez-Montero Luis Miguel
Published: September 2021

In this work, a new medical image encryption/decryption algorithm was proposed. It is based on three main parts: the Jigsaw transform, Langton’s ant, and a novel way to add deterministic noise. The Jigsaw transform was used to hide visual information effectively, whereas Langton’s ant and the deterministic noise algorithm give a reliable and secure approach. As a case study, the proposal was applied to high-resolution retinal fundus images, where a zero mean square error was obtained between the original and decrypted image. The method performance has been proven through several testing methods, such as statistical analysis (histograms and correlation distributions), entropy computation, keyspace assessment, robustness to differential attack, and key sensitivity analysis, showing in each one a high security level. In addition, the method was compared against other works showing a competitive performance and highlighting with a large keyspace (>1×101,134,190.38). Besides, the method has demonstrated adequate handling of high-resolution images, obtaining entropy values between 7.999988 and 7.999989, an average Number of Pixel Change Rate (NPCR) of 99.5796%±0.000674, and a mean Uniform Average Change Intensity (UACI) of 33.4469%±0.00229. In addition, when there is a small change in the key, the method does not give additional information to decrypt the image.
Suitable Interpretation of Skin Prick Test and Biomedical Guidance Leads to a Better Clinical State in Atopic Individuals with High Indoor Permanence: Possible Therapeutic Implications
Authors: Nakamatsu Bernardo Yassunobu , Fernandes Archangelo Padreca , da Silva Débora , Santos Ludimila Souza , de Sousa Thamires Rodrigues , Victor Jefferson Russo
Published: September 2021

Indoor conditions contribute to allergen sensitization and multiple allergens reactivity, mainly for inhaled allergens. This study analyzes if Skin Prick Test (SPT) combined with efficient individual biomedical guidance about allergy development’s social, biological, and environmental aspects can yield a better clinical state with therapeutic implications for atopic individuals with high indoor permanence. We recruited atopic and non-atopic volunteers (clinically and in vitro diagnosed) with indoor permanence above 15 h per day and without previous SPT evaluation. The SPT and serum anti-allergen IgE analyses were performed individually in person, demonstrated, and discussed by the practitioners. Six months after, SPT and specific IgE titers determination were repeated, and a questionnaire to evaluate the effectiveness of the practitioner’s orientation was performed. After six months, 14% of atopic volunteers reported changes in their social habits, 30% said that they avoid the development of allergies clinical symptoms, and 68% reported a substantial improvement in their health after being informed mentored about their allergen reactivity. The control non-atopic group, as expected, reported no changes in social habits, the maintenance of total avoidance of allergic symptoms, and almost no improvement of their health. Reduced SPT and serum allergen-specific IgE titers were detected in the atopic individuals corroborating with questionnaire results. Our results indicated that SPT, followed by an individual and efficient discussion about the main biomedical aspects of allergy development, could exert a pronounced therapeutic role in allergy development by high indoor permanence individuals.
Interactions of the Receptor Binding Domain of SARS-CoV-2 Variants with hACE2: Insights from Molecular Docking Analysis and Molecular Dynamic Simulation
Authors: Celik Ismail , Yadav Rohitash , Duzgun Zekeriya , Albogami Sarah , El-Shehawi Ahmed M. , Fatimawali Fatimawali , Idroes Rinaldi , Tallei Trina Ekawati , Emran Talha Bin
Published: September 2021

Since the beginning of the coronavirus 19 (COVID-19) pandemic in late 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been evolving through the acquisition of genomic mutations, leading to the emergence of multiple variants of concern (VOCs) and variants of interest (VOIs). Currently, four VOCs (Alpha, Beta, Delta, and Gamma) and seven VOIs (Epsilon, Zeta, Eta, Theta, Iota, Kappa, and Lambda) of SARS-CoV-2 have been identified in worldwide circulation. Here, we investigated the interactions of the receptor-binding domain (RBD) of five SARS-CoV-2 variants with the human angiotensin-converting enzyme 2 (hACE2) receptor in host cells, to determine the extent of molecular divergence and the impact of mutation, using protein-protein docking and dynamics simulation approaches. Along with the wild-type (WT) SARS-CoV-2, this study included the Brazilian (BR/lineage P.1/Gamma), Indian (IN/lineage B.1.617/Delta), South African (SA/lineage B.1.351/Beta), United Kingdom (UK/lineage B.1.1.7/Alpha), and United States (US/lineage B.1.429/Epsilon) variants. The protein-protein docking and dynamics simulation studies revealed that these point mutations considerably affected the structural behavior of the spike (S) protein compared to the WT, which also affected the binding of RBD with hACE2 at the respective sites. Additional experimental studies are required to determine whether these effects have an influence on drug–S protein binding and its potential therapeutic effect.