bluuebunny/crossref_metadata_2025 huggingface dataset:
Global Mapping and Visualization Analysis of One Health Knowledge in the COVID-19 Context
Authors: Zyoud Shaher
Published: March 2024

Globally, the COVID-19 pandemic had a significant impact on the health, social, and economic systems, triggering lasting damage and exposing the complexity of the problem beyond just being a health emergency. This crisis has highlighted the need for a comprehensive and collaborative strategy to successfully counter infectious diseases and other global challenges. With the COVID-19 pandemic pushing One Health to the forefront of global health and sustainable development agendas, this concept has emerged as a potential approach for addressing these challenges. In the context of COVID-19, this study investigates global knowledge about One Health by examining its state, significant contributions, and future directions. It seeks to offer an integrated framework of insights guiding the development of well-informed decisions. A comprehensive search using the Scopus database was conducted, employing specific terms related to One Health and COVID-19. VOSviewer 1.6.19 software was used to generate network visualization maps. Countries’ research output was adjusted based on their gross domestic product (GDP) and population size. The study identified a total of 527 publications. The United States led with 134 documents (25.4%), but India topped the adjusted ranking. One Health journal stood as the most common outlet for disseminating knowledge (49 documents; 9.3%), while Centers for Disease Control and Prevention (CDC), the United States emerged as the most prolific institution (13 documents; 2.5%). Key topics were related to the virus transmission mechanisms, climate change impacts, antimicrobial resistance, ecosystem health, preparedness, collaboration, community engagement, and developing of efficient surveillance systems. The study emphasizes how critical it is to capitalize on the present momentum of COVID-19 to advance One Health concepts. Integrating social and environmental sciences, and a variety of professions for better interaction and collaboration is crucial. Additionally, increased funding for developing countries, and legislative empowerment are vital to advance One Health and boost disease prevention.
First-interview response patterns of intensive longitudinal psychological and health data
Authors: Blozis Shelley A
Published: March 2024

Self-report data are essential in health psychology research where an individual’s perception is critical to understanding one’s health and psychological status. Intensive data collection over time, including daily diary assessments, is necessary in understanding within- and between-person variability in health and psychological processes over time. An “initial elevation or latent decline” (IELD) effect, inherent of self-report data, is increasingly acknowledged in the social psychology literature, but awareness of this effect in health psychology research is lacking, particularly in studies that emphasize within- and between-person variability in self-reports. The IELD effect is a pattern in which responses tend to be more extreme at the initial interview relative to subsequent responses. This paper illustrates the impact of IELD in applications of mixed-effects models based on observational self-reports and concludes that researchers take such effects into account in data analysis or in the research designing phase to help mitigate such effects.
The tragedy of utopia in the age of the Anthropocene: Beyond dystopia, despair and catastrophic futures
Authors: Featherstone Mark
Published: March 2024

My key objective in this article is to explore the history of the concept of utopia and its application in really existing social, political, economic and cultural forms. Starting with a consideration of what I call the economy of utopia, I theorise the desire for the ideal society in terms of a deeply human drive to seek to overcome vulnerability, limitation and finitude that is set upon failure and the fall towards dystopia by virtue of the fact that it is this very lack that defines the being of the human animal. Following this section of the article, in the second part of my piece, I move on to trace the history of utopia from the visions of Ancients, through the idealism of the Moderns, up to the champions of the theory of the end of history and a utopian version of capitalism. Finally, in conclusion, I focus on the catastrophic impacts of this global utopia of capitalism realised in the form of the Anthropocene and imagine a truly human, tragic utopia founded upon a recognition of our constitutive lack, vulnerability and finitude.
Research on the deformation and settlement characteristics of tunnel lining structures based on distributed fibre optic sensing technology
Authors: Wu Fengyuan , Sheng Wei , Zhang Guanhua , Li Hongnan , Ren Yuhao , Zhang Kexin , Wang Chao , Sun Tong
Published: March 2024

To study the deformation and settlement characteristics of tunnel lining structures, a tunnel lining structure model was designed based on distributed fibre optic sensing technology. Compared to the cylindrical model used in traditional tunnel lining structural model experiments, in this study, a reinforced concrete structural model was adopted, which can embed fibre optics in the structure, which is closer to actual tunnel engineering conditions. Central and symmetrical concentrated loading experiments were carried out with a simply supported reaction frame. The results of the distributed fibre optic monitoring were compared and analysed with those of traditional monitoring methods to verify the reliability of the distributed fibre optic monitoring results. The numerical simulations of the experiments were conducted by using finite element analysis. By comparing and analysing the simulation and experimental results, the correctness of the simulation calculation results were verified. On this basis, the impact of concrete strength, circumferential reinforcement spacing, and longitudinal reinforcement strength on the deformation and settlement of the tunnel lining structure were analysed. The results show that the hierarchical effect of the strain monitoring results obtained by the embedded fibre optic is more obvious, indicating that the radial monitoring effect of the embedded fibre optic on the tunnel structure is less affected by other external factors than the strain gauge, and the monitoring data are more accurate and effective, with good engineering characteristics. Improving the concrete strength, appropriate circumferential reinforcement spacing, and increasing the longitudinal reinforcement strength can effectively enhance the ability of the structure to resist deformation at the stress location. These factors play a significant role in improving the overall resistance to deformation and safety of the structure. The research results provide a theoretical basis and experimental data for the application of distributed fibre optics in monitoring the deformation and settlement of tunnel lining structures.
Effect of reaction temperature on the corrosion behaviour of Inconel alloys in lithium molten salt under an oxidising atmosphere
Authors: Choi Woo-Seok , Lim Kyu-Seok , Kim Wan-Bae , Cho Soo-Haeng , Lee Jong-Hyeon
Published: March 2024

The effect of temperature on the corrosion behaviour of Inconel alloys employed for the electroreduction of metal oxides to metals in a lithium molten salt was investigated. The ratio of the weight loss of Inconel 601 and 690 to that of Inconel 600, Inconel 601 and 690 was approximately four and six at 650 °C, and five and eight times higher than that of Inconel 600 above 750 °C for 72 h, respectively. The corrosion products of all the alloys tested at 650 °C and 750 °C for 72 h were mainly Cr2O3 and FeCr2O4; however, only Cr2O3 was obtained when Inconel 690 was corroded at 750 °C or higher. Additionally, an alloy with a composition of max. 16.3 wt.% Cr, min. 73.66 wt.% Ni and well below 8.15 wt.% Fe was expected to exhibit excellent corrosion resistance in a lithium molten salt system at 650–850 °C.
The role of probiotics in improving menstrual health in women with primary dysmenorrhoea: A randomized, double-blind, placebo-controlled trial (the PERIOD study)
Authors: Zakaria Izyan Atiqah , Mohammed Zain Nur Aini , Teik Chew Kah , Abu Muhammad Azrai , Zainuddin Ani Amelia , Abdul Aziz Nor Haslinda , Safian Nazarudin , Mohd Mokhtar Norfilza , Raja Ali Raja Affendi , Beng Kwang Ng , Mohamed Ismail Nor Azlin , Hamizan Muhammad Rafiuddin , Ab Razak Wira Sorfan , Nur Azurah Abdul Ghani
Published: March 2024

Background:

Primary dysmenorrhea is associated with poorer quality of life; however, the causal mechanism remains unclear. A vast body of literature supports the use of oral probiotics for relief from the symptoms of endometriosis; however, to our knowledge, no study has prescribed probiotics for primary dysmenorrhea.

Objective:

The aim of this study is to investigate the effects of 3-month supplementation with oral probiotics on quality of life and inflammatory markers in women with primary dysmenorrhea.

Design:

Randomized placebo-controlled trial.

Methods:

A total of 72 patients (36 patients in each arm) were randomized to receive either oral sachets containing 5 billion colony-forming units each of Lactobacillus acidophilus BCMC (BCrobes Microbial Cells) 12130, Lactobacillus casei subsp BCMC 12313, Lactobacillus lactis BCMC 12451, Bifidobacterium bifidum BCMC 02290, Bifidobacterium longum BCMC 02120, and Bifidobacterium infantis BCMC 02129 each or placebo twice daily for 3 months. Main outcome measures were visual analog scale, verbal rating scale, physical and mental health scores using Short-Form 12-Item version 2 questionnaire, frequency of nonsteroidal anti-inflammatory drug use, and changes in inflammatory markers (interleukin-6, interleukin-8, and tumor necrosis factor alpha) before and after treatment.

Results:

There was no significant difference in the quality of life scores between the probiotic and placebo groups. Both groups showed significant improvement in pain (visual analog scale) and severity (verbal rating scale) scores but the probiotic group had much lower nonsteroidal anti-inflammatory drug use (odds ratio: 0.69, 95% confidence interval: 0.26–1.83) and better mental health scores (mean change: 6.5, p = 0.03 versus 6.1, p = 0.08) than the placebo group. There was a significant confounding effect of nonsteroidal anti-inflammatory drug use on quality of life scores. No significant difference was found in inflammatory cytokines.

Conclusion:

Tested oral probiotics improved mental health and potentially reduced the use of nonsteroidal anti-inflammatory drugs; however, there was no significant change in inflammatory markers. Further research with a larger sample size is needed to confirm the findings.

Registration:

This study is registered under ClinicalTrials.gov (NCT04119011).
Seroprevalence of hepatitis B virus infection and factors associated among pregnant women in Ethiopia: A systematic review and meta-analysis
Authors: Asgedom Yordanos Sisay , Kassie Gizachew Ambaw , Woldegeorgis Beshada Zerfu , Meskele Koyira Mengistu , Kebede Tsegaye Melaku
Published: March 2024

Background:

Hepatitis B virus infection is a major public health problem among pregnant women worldwide. Hepatitis B virus is highly infectious and is the most common cause of morbidity and mortality among pregnant women, and evidence is scarce on the pooled seroprevalence of hepatitis B virus in Ethiopia.

Objectives:

This study aimed to investigate the pooled seroprevalence of hepatitis B virus infection and factors associated with pregnant women in Ethiopia.

Design:

A systematic review and meta-analysis was employed in accordance with the Preferred Reporting Items for Systematic Reviews.

Data sources:

Searches were carried out in biomedical databases such as PubMed/Medline, Science Direct, Web of Science, Google Scholar, Hinari, and the Cochrane Library published in English until June 2023.

Methods:

Observational study designs were selected. Endnote citation manager was used to collect and organize the search outcomes and remove duplicate articles. The data were extracted using a Microsoft Excel spreadsheet and exported to STATA 16.0 software for the analysis.

Results:

A total of 48 research articles were included in the final analysis. The pooled estimated sero prevalence of hepatitis B virus infection among pregnant women in Ethiopia was 5.78% (95% confidence interval = 5.14, 6.43). History of abortion (odds ratio = 6.56, 95% confidence interval = 4.88, 8.90), history of blood transfusion (odds ratio = 5.74, 95% confidence interval = 4.04, 8.16), history of hospitalization (odds ratio = 5.40, 95% confidence interval = 3.68, 7.94), history of multiple sexual partner (odds ratio = 5.80, 95% confidence interval = 3.71, 9.05), history of surgical procedure (odds ratio = 7.39, 95% confidence interval = 4.16, 13.14), history of tattooing (odds ratio = 4.59, 95% confidence interval = 2.83, 7.43), and history of tooth extraction (odds ratio = 4.46, 95% confidence interval = 2.42, 8.22) were significantly associated with hepatitis B virus infection among pregnant women in Ethiopia.

Conclusion:

The overall pooled prevalence of hepatitis B virus infection among pregnant women in Ethiopia is relatively high. Having a history of abortion, blood transfusion, hospitalization, multiple sexual partners, surgical procedures, tattooing, and tooth extraction were found to be risk factors for hepatitis B virus. Therefore, extensive screening programs for hepatitis B virus in all pregnant women in Ethiopia are needed to prevent further infection and decrease the vertical transmission caused by the disease.

Registration number:

PROSPERO CRD: 42023438522
Teachers’ Perceptions of Mathematical Discourse
Authors: Hayden Stacy M. , Kearney Kelly , Gubbins E. Jean
Published: March 2024

This article analyzes teachers’ perceptions and experiences using an educative curriculum designed to promote high levels of mathematical discourse. After participating in 2 days of professional learning, grade 3 teachers implemented a predifferentiated and enriched mathematics unit in their classroom. The curriculum was designed to be educative, meaning that teaching it served as professional learning. During implementation, researchers conducted an observation of each treatment teacher and after implementation was complete teachers participated in a post interview about their experiences. Findings from this qualitative study indicate that after teaching the curriculum teachers understood the value of oral discourse and how to implement it in their classroom and could identify student benefits from participating in this type of curriculum. This study illuminates promising practices that may support teachers in implementing research-based best practices.
An occurrence of eosinophilic folliculitis and alopecia associated with a sustained complete response to mogamulizumab in Sézary syndrome: a case report
Authors: L’Orphelin Jean-Matthieu
Published: March 2024

Mogamulizumab is a monoclonal antibody that binds to C–C chemokine receptor 4 (CCR4), initiating antibody-dependent cellular cytotoxicity. CCR4 is highly expressed in the cutaneous T-cell lymphoma subtypes mycosis fungoides and Sézary syndrome (SS), and mogamulizumab has been shown to be effective in patients with these conditions who were refractory to at least one prior systemic treatment. One of the more common adverse events encountered with mogamulizumab is rash, which may mimic disease progression and lead to premature discontinuation. Moreover, there has been some evidence to suggest that mogamulizumab-associated rash (MAR) is associated with improved outcomes in some patients, particularly those with SS. This report presents the case of a 72-year-old woman with SS, which manifested with macular and papular lesions and abnormal blood cytometry, who was treated with mogamulizumab after failure of bexarotene and photopheresis combination therapy. She achieved a complete response (CR), but experienced lymphopenia associated with histologically proven eosinophilic folliculitis (EF) of the scalp and alopecia. The EF responded well to initial topical corticosteroids, defined by regression of erythema and pustular involvement and reduction in pruritus-like symptoms, but without hair regrowth. Mogamulizumab was withdrawn after 32 cycles, but CR was maintained. To date, EF persists in the form of diffuse erythema without pustules or pruritus. A link between cluster of differentiation 4 lymphopenia and EF has previously been established; therefore, EF should be considered in patients who develop rash and lymphopenia while receiving treatment with mogamulizumab. MAR has been associated with clinical response to mogamulizumab, and this case report adds to the evidence that EF may also be associated with sustained clinical response following treatment cessation. However, regular monitoring is required to prevent a relapse of SS. Prospective studies are needed to confirm whether such an association between EF and CR following mogamulizumab exists.
Lymph node harvesting after laparoscopic complete mesocolic excision colectomy in colon cancer with practical application of glacial acid, absolute ethanol, water, and formaldehyde solution: A prospective cohort study
Authors: Nguyen Minh Thao , Dang Cong Thuan , Song Nguyen Tran Bao , Pham Nguyen Cuong , Le Dinh Duong , Pham Minh Duc , Nguyen Huu Tri , Dung Phan Dinh Tuan , Phu Nguyen Doan Van , Nguyen Thanh Phuc , Doan Phuoc Vung , Nguyen Dinh Son , Pham Anh Vu
Published: March 2024

Objectives:

Quality of surgery has recently become an essential topic in the prognosis of colon cancer. Complete mesocolic excision for colon cancer has recently gained popularity with high-quality surgery. Patient specimens after complete mesocolic excision with central vessel ligation procedures have an integrity of the mesocolon and the yield of three fields of lymph node harvest. We apply the glacial acid, absolute ethanol, water, and formaldehyde solution to each specimen based on the Japanese classification of lymph node groups and station numbers. We aim to identify the distribution and status of lymph node metastasis according to each tumor site and some pathological characteristics related to this disease.

Methods:

A prospective cohort study was performed on 45 laparoscopic complete mesocolic excision surgery patients.

Results:

2791 lymph nodes were harvested after complete mesocolic excision surgery. The average number was 62.0 ± 22.3 nodes. The mean tumor size (in the largest dimension) was 4.2 ± 1.8 cm. The average length of the resected bowel segments was 29.1 ± 7.7 cm. There are 63 (2.3%) node metastases in 2791 lymph nodes, in which 17/45 (37.8%) patients had pN(+). The minimum positive node size was 1 mm. The positive pericolic lymph nodes (station 1) accounted for the highest rate, with 53 nodes (1.9%). The number of lymph nodes in young age ⩽60 is more significant than in older. The results were similar, with a more significant node retrieval in the group with a tumor size >4.5 cm and specimen length >25 cm. The number of lymph nodes in lower tumor invasive (pT1,3) was smaller than pT4. Our research shows that the cecum, ascending, and descending colon had greater nodes than others, with a mean number of 78.6, 74.2, and 71.3, respectively.

Conclusions:

The metastasis and harvested lymph nodes accounted for the highest rate of colon cancer in station 1 and the lowest rate in station 3. The number of retrieved lymph nodes was significantly associated with tumor location, size, specimen length, and patient age.
Effects of semi-immersive virtual reality exercise on the quality of life of community-dwelling older adults: Three-month follow-up of a randomized controlled trial
Authors: Wang Li-Ting
Published: March 2024

Objective

This study aimed to examine the effects of semi-immersive virtual reality (VR)-based exercise on the quality of life of older adults.

Methods

It used a randomized controlled trial design. Older adults (mean age: 72.16 ± 4.9 years) were randomly assigned to experimental ( n = 48) and control ( n = 50) groups. The experimental group engaged in semi-immersive VR exercise for 75–90 min, twice a week, for 12 weeks and partook in no other intervention between the end of the exercise intervention and follow-up. Control group members did not participate in any similar program during the intervention or follow-up periods. Both groups completed three assessments: at baseline (pre-test), post-intervention (post-test), and 3 months later (follow-up). Quality of life was assessed using the World Health Organization Quality of Life Instrument-Older Adults Module.

Results

Generalized estimating equation analyses indicated that the experimental group exhibited significant post-intervention improvements in quality of life in terms of sensory ability, autonomy, social participation/isolation, death and dying domain, and overall quality of life scores. However, none of these significant effects were maintained 3 months after exercise intervention cessation.

Conclusions

Semi-immersive VR exercise may be a feasible strategy toward enhancing the quality of life of older adults. However, the participants’ quality of life was not maintained upon exercise cessation, indicating that older adults need to be encouraged to exercise regularly to maintain a good quality of life. VR may need to be combined with other modes of intervention in the future to facilitate long-term quality-of-life improvement in older adults.
Feminist alternative practices among independent artists: The case of Guangzhou, China
Authors: Liu Ruoxi
Published: March 2024

Drawing from seven months of fieldwork among independent artists and their communities in Guangzhou, China, in 2020–2021, this paper investigates the feminist alternative practices in response to the experiences of gender marginalisation of independent artists. Along with being sexualised and discouraged by some of their art colleagues and the public, there has been an emergence of alternative practices among female independent artists in Guangzhou, including alternative art production, space cultivation, and community development. Alternative art practices have not only diversified the expression and representation of female artists; they have also helped female and non-binary people connect to discuss gender-related issues and provide mutual support. Altogether, these expanding feminist practices, spaces, and communities have yielded everyday life strategies to negotiate and contest existing patriarchal conventions and imbalanced power relationships. My account also offers a view of changing gender politics within art communities in Guangzhou’s independent art field.
Using Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration for the Diagnosis and Treatment of a Mediastinal Mass With Eccentric Calcifications: A Case Report
Authors: Ghalayni Ruba , Gowda Satish
Published: March 2024

Mediastinal masses present a diagnostic challenge due to their similar imaging characteristics, making distinguishing between noninfectious and infectious processes or malignancies difficult. A mediastinal abscess can result in severe life-threatening infections if left untreated. Traditional treatment approaches involve surgical debridement and drainage; however, emerging endobronchial techniques, such as endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA), offer a less-invasive means of diagnosing and managing abscesses. Herein, we describe a case of a young male patient who exhibited nonspecific symptoms, including pleuritic chest pain, shortness of breath, and fever. Imaging revealed a mediastinal mass with granuloma formation. EBUS-TBNA successfully drained the abscess, and microbiology analysis confirmed the growth of Streptococcus intermedius. Subsequently, his symptoms resolved, and follow-up imaging demonstrated the resolution of the mass and associated calcifications. Further research is warranted to assess the role of EBUS-TBNA in benign mediastinal masses with granuloma formation.
Estimates of Teach for America Effects on Student Test and Nontest Academic Outcomes Over Time
Authors: Backes Ben , Hansen Michael
Published: March 2024

This article examines the impact of Teach for America (TFA) on following-year student test and nontest outcomes in Miami-Dade County Public Schools. Using data from 2010 through 2021, this article measures the extent to which student exposure to TFA is followed by improved outcomes in the future, relative to students with other early-career teachers in the same school. In particular, this article measures days missed due to absences or suspensions, course grades in each core subject, and progression in math courses. We find that students taught by TFA math teachers go on to have higher grades in math courses in the following year and are less likely to miss school due to being absent or suspended. However, while students in TFA classrooms score higher on math and ELA assessments in a given year, these test score gains fade out by the following year.
Can Multi-organ Sonographic Parameters Predict Mortality in Patients With Acute Kidney Injury?
Authors: Aslaner Mehmet Ali , Cerit Mahi Nur , Esen Şaban
Published: March 2024

Objective:

This study aimed to investigate sonographic parameters that could predict 1-month mortality in patients with acute kidney injury (AKI).

Materials and Methods:

This study was a post-hoc analysis of multi-organ sonographic examinations conducted between August 2020 and July 2021. Adult patients who presented to the emergency department (ED) with AKI were recruited. Multi-organ (lung, cardiac, inferior vena cava, kidney, and bladder) sonographic examinations were performed by emergency physicians and evaluated by a radiologist, cardiologist, and another emergency physician, while being anonymized to the results.

Results:

There were 165 patients with AKI enrolled in the study, and 40 (24.2%) of them experienced 1-month mortality. Following univariate analyses of 23 sonographic parameters, multivariate regression analysis revealed that diffuse ascites (odds ratio [OR], 3.67; 95% confidence interval [CI]: 1.27–10.61) and left ventricular basal diameter (OR, 0.93; 95% CI: 0.87–0.99) were related with 1-month mortality in patients with AKI.

Conclusion:

In this cohort of AKI patients, the most powerful sonographic parameter, as a predictor of 1-month mortality, was diffuse ascites. Further clinical studies are needed to validate and refine these diagnostic findings.
Long noncoding RNA GATA2AS influences human erythropoiesis by transcription factor and chromatin landscape modulation
Authors: Liu Guoyou , Kim Juhyun , Nguyen Nicole , Zhou Lecong , Dean Ann
Published: March 2024

Long noncoding RNAs (lncRNAs) are extensively expressed in eukaryotic cells and have been revealed to be important for regulating cell differentiation. Many lncRNAs have been found to regulate erythroid differentiation in the mouse. However, given the low sequence conservation of lncRNAs between mouse and human, our understanding of lncRNAs in human erythroid differentiation remains incomplete. lncRNAs are often transcribed opposite to protein coding genes and regulate their expression. Here, we characterized a human erythrocyte-expressed lncRNA, GATA2AS, which is transcribed opposite to erythroid transcription regulator GATA2. GATA2AS is a 2080-bp long, primarily nucleus-localized noncoding RNA that is expressed in erythroid progenitor cells and decreases during differentiation. Knockout of GATA2AS in human HUDEP2 erythroid progenitor cells using CRISPR-Cas9 genome editing to remove the transcription start site accelerated erythroid differentiation and dysregulated erythroblast gene expression. We identified GATA2AS as a novel GATA2 and HBG activator. Chromatin isolation by RNA purification showed that GATA2AS binds to thousands of genomic sites and colocalizes at a subset of sites with erythroid transcription factors including LRF and KLF1. RNA pulldown and RNA immunoprecipitation confirmed interaction between GATA2AS and LRF and KLF1. Chromatin immunoprecipitation sequencing (ChIP-seq) showed that knockout of GATA2AS reduces binding of these transcription factors genome wide. Assay for transposase-accessible chromatin sequencing (ATAC-seq) and H3K27ac ChIP-seq showed that GATA2AS is essential to maintain the chromatin regulatory landscape during erythroid differentiation. Knockdown of GATA2AS in human primary CD34+ cells mimicked results in HUDEP2 cells. Overall, our results implicate human-specific lncRNA GATA2AS as a regulator of erythroid differentiation by influencing erythroid transcription factor binding and the chromatin regulatory landscape.
DVA: predicting the functional impact of single nucleotide missense variants
Authors: Wang Dong , Li Jie , Wang Edwin , Wang Yadong
Published: March 2024

Background

In the past decade, single nucleotide variants (SNVs) have been identified as having a significant relationship with the development and treatment of diseases. Among them, prioritizing missense variants for further functional impact investigation is an essential challenge in the study of common disease and cancer. Although several computational methods have been developed to predict the functional impacts of variants, the predictive ability of these methods is still insufficient in the Mendelian and cancer missense variants.

Results

We present a novel prediction method called the disease-related variant annotation (DVA) method that predicts the effect of missense variants based on a comprehensive feature set of variants, notably, the allele frequency and protein–protein interaction network feature based on graph embedding. Benchmarked against datasets of single nucleotide missense variants, the DVA method outperforms the state-of-the-art methods by up to 0.473 in the area under receiver operating characteristic curve. The results demonstrate that the proposed method can accurately predict the functional impact of single nucleotide missense variants and substantially outperforms existing methods.

Conclusions

DVA is an effective framework for identifying the functional impact of disease missense variants based on a comprehensive feature set. Based on different datasets, DVA shows its generalization ability and robustness, and it also provides innovative ideas for the study of the functional mechanism and impact of SNVs.
Whole-genome sequencing and comparative genomics reveal candidate genes associated with quality traits in Dioscorea alata
Authors: Mota Ana Paula Zotta , Dossa Komivi , Lechaudel Mathieu , Cornet Denis , Mournet Pierre , Santoni Sylvain , Lopez David , Chaïr Hana
Published: March 2024

Background

Quality traits are essential determinants of consumer preferences. Dioscorea alata (Greater Yam), is a starchy tuber crop in tropical regions. However, a comprehensive understanding of the genetic basis underlying yam tuber quality remains elusive. To address this knowledge gap, we employed population genomics and candidate gene association approaches to unravel the genetic factors influencing the quality attributes of boiled yam.

Methods and Results

Comparative genomics analysis of 45 plant species revealed numerous novel genes absent in the existing D. alata gene annotation. This approach, adding 48% more genes, significantly enhanced the functional annotation of three crucial metabolic pathways associated with boiled yam quality traits: pentose and glucuronate interconversions, starch and sucrose metabolism, and flavonoid biosynthesis. In addition, the whole-genome sequencing of 127 genotypes identified 27 genes under selection and 22 genes linked to texture, starch content, and color through a candidate gene association analysis. Notably, five genes involved in starch content and cell wall composition, including 1,3-beta Glucan synthase, β-amylase, and Pectin methyl esterase, were common to both approaches and their expression levels were assessed by transcriptomic data.

Conclusions

The analysis of the whole-genome of 127 genotypes of D. alata and the study of three specific pathways allowed the identification of important genes for tuber quality. Our findings provide insights into the genetic basis of yam quality traits and will help the enhancement of yam tuber quality through breeding programs.
Investigating the mortality trend of gastrointestinal cancers in Babol, North Iran (2013–2021)
Authors: Ebrahimi Pouyan , Karami Mohsen , Delavari Sahar , Shojaie Layla , Hosseini-Berneti Seyed-Hossein , Bayani Fatemeh , Moghaddasi Mehdi , Babazade Ozra , Nikbakht Hossein-Ali
Published: March 2024

Background and aim

This study aims to examine the mortality rate and trend of gastrointestinal cancers, particularly gastric cancer, as the leading cause of death among cancers in northern Iran over a 9-year period. In light of the changing incidence and mortality rates of cancer in Iran and around the world, the importance of these diseases in people’s lives, and the necessity of updating and monitoring the trend of cancer mortality, we have decided to report on the mortality trend of gastrointestinal cancers, based on crude and age-standardized rates.

Method

This study is a cross-sectional examination of deaths caused by gastrointestinal cancers in Babol city, Iran, between 2013 and 2021. Data was collected from the cause of death registration and classification system of Babol University of Medical Sciences. Population estimation was obtained from the latest census reports. The crude and age-standardized mortality rates and trends of the cancers were calculated.

Results

Overall, there were 1345 deaths from gastrointestinal cancers with an average age of 69.11 ± 14.25 years. The crude and age-standardized rates of these cancers rose from 24.1 to 20.1 per hundred thousand people in 2012 to 29.5 and 25.5 per hundred thousand people, respectively. This trend became more prevalent significantly with the increase of each decade of age for both men (P-value Trend = 0.002) and women (P-value Trend = 0.012). An analysis of gastrointestinal cancers revealed a decreasing trend for cancers of the small intestine, an increasing trend for cancers of the colon, pancreas, and gallbladder, and a stable trend for the remaining cancers over the study period.

Conclusion

The age-standardized rate and the number of gastrointestinal cancers is rising, highlighting the importance of preventative measures such as screening, increasing public awareness, and appropriate diagnostic methods.
Chronic disseminated candidiasis in a patient with acute leukemia - an illustrative case and brief review for clinicians
Authors: Graeter Allison , Lee Dasom , Handley Guy , Baluch Aliyah , Klinkova Olga
Published: March 2024

Chronic disseminated candidiasis (CDC) is a severe but rarely seen fungal infection presenting in patients with hematologic malignancies after a prolonged duration of neutropenia. A high index of suspicion is required to diagnose CDC as standard culture workup is often negative. While tissue biopsy is the gold standard of diagnosis, it is frequently avoided in patients with profound cytopenias and increased bleeding risks. A presumptive diagnosis can be made in patients with recent neutropenia, persistent fevers unresponsive to antibiotics, imaging findings of hypoechoic, non-rim enhancing target-like lesions in the spleen and liver, and mycologic evidence. Here, we describe the case of an 18-year-old woman with relapsed B-cell acute lymphoblastic leukemia treated with re-induction chemotherapy who subsequently developed CDC with multi-organ involvement. The diagnosis was made based on clinical and radiologic features with positive tissue culture from a skin nodule and hepatic lesion. The patient was treated for a total course of 11 months with anti-fungal therapy, most notably amphotericin B and micafungin, and splenectomy. After initial diagnosis, the patient was monitored with monthly CT abdomen imaging that showed disease control after 5 months of anti-fungal therapy and splenectomy. The diagnosis, treatment, and common challenges of CDC are outlined here to assist with better understanding, diagnosis, and treatment of this rare condition.
Demographic characteristics and mental health condition of Tehran Municipality employees during the COVID-19 pandemic
Authors: Kalhori Elham , Khodakarami Nahid , Hamdieh Mostafa , Gholami Roya , Dashti Sareh
Published: March 2024

Background

Coronavirus disease 2019 (COVID-19) has become a global health issue with various effects on the physical and mental state of the societies. The aim of this study was to identify the demographic characteristics and mental health condition of Tehran Municipality employees during the COVID-19 pandemic.

Methods

This cross-sectional study was performed on Tehran Municipality employees in 2020–2021. Participants were selected using stratified random sampling and were divided into COVID-19 and uninfected groups. Demographic characteristics, COVID-19 risk behaviors, General Health Questionnaire-28 (GHQ-28), and Well- Being Social Inventory were filled for all participants.

Results

A total of 510 participants (363 uninfected participants and 147 participants with COVID-19) were evaluated. The prevalence of female gender was significantly higher in COVID-19 group compared to uninfected group (p < 0.001). There was a significant difference between groups in terms of education level (p < 0.001), prevalence of excess weight (p < 0.001), and working sector (p < 0.001). The uninfected group mainly had low contact with clients (p < 0.001) and few underlying diseases (p = 0.004) compared to the COVID-19 group. The mean GHQ-28 and Well- Being Social Inventory were significantly higher in the uninfected group compared to the COVID-19 group (p = 0.002 and p < 0.001, respectively). The prevalence of no and low contact level was significantly higher in the high infection cluster compared to moderate and low infection clusters (p = 0.024).

Conclusions

The findings of this study indicated that all workers should be educated about the significance of social distancing and follow the recommendations regardless of their level of contact with clients.
Tropheryma whipplei infection in the lung of a patient with long COVID: a case report
Authors: Ruan Wenjing , Xu Jing , Yang Fan , Wu Xiaohong , Ying Kejing
Published: March 2024

Background

Immune dysregulation in individuals with long COVID has been detected. Differential diagnosis of diffuse infiltration on chest CT in long COVID is challenging.

Case presentation

A 62-year-old man presented with a 10-month history of dyspnea after COVID-19 infection. Dyspnea became worse in the one month preceding presentation. The chest CT showed multifocal, subpleural, bilateral opacities due to long-COVID, and infiltration around the bronchovascular bundle in the bilateral lower lung field. The pathology for the transbronchial cryobiopsy (TBCB) first reported chronic inflammation (mainly interstitial pneumonia). The patient had positive results on tests for the antibody, RO-52+, EJ+. The presumptive diagnosis of connective tissue disease-interstitial lung disease was made. Prednisone and cyclophosphamide were given. At follow-up one month later, the chest CT showed new diffuse ground-glass infiltration. The previous TBCB specimen was re-evaluated. Foamy macrophages were found in the alveolar air space. Periodic acid-Schiff (PAS) staining was performed. Numerous intracytoplasmic organisms were detected, with morphologic features consistent with those of Tropheryma whipplei. The patient recovered after intravenous ceftriaxone and oral trimethoprim-sulfamethoxazole. The final diagnosis was lung T. whipplei infection and long COVID-19.

Conclusion

This is the first case report of Tropheryma whipplei infection in the lung of a patient with long COVID-19. T. whipplei should be considered as a potential pathogen for diffuse lung infiltration in the post-COVID-19 era.
Socio-demographic determinants of the knowledge of Monkeypox Virus among the general public: a cross-sectional study in a Tertiary Care Center in Nepal
Authors: Das Santa Kumar , Bhattarai Abhinav , Paudel Kiran , Bhusal Sandesh , Shah Sangam , Timsina Sakchhyam , Subedi Aastha , Niroula Sandhya , Alshahrani Najim Z. , Sah Sanjit , Padhi Bijaya Kumar , Barboza Joshuan J. , Rodriguez-Morales Alfonso J. , Salas-Matta Luis A. , Bonilla-Aldana D. Katterine , Sah Ranjit
Published: March 2024

Background and objective

Monkeypox virus (MPXV) is the causative agent of monkeypox’s zoonotic infection and was declared a global emergency by the World Health Organization (WHO). Studies from different countries have shown insufficient knowledge among the general public on MPXV. This study aimed to assess the knowledge of the general public of Nepal on MPXV.

Methods

Three hundred people were interviewed in person in October 2022, and 282 complete responses were recorded. The questionnaire related to the knowledge of MPXV was derived from a previous study conducted among the general population of Saudi Arabia. Twenty-two questions were included that assessed the knowledge and attitude of Nepalese toward monkeypox. Statistical comparison between high and low knowledge was performed using Pearson’s Chi-square test. Logistic regression models were deployed to establish the relationship between participants’ knowledge and socio-demographic characteristics.

Results

Among the total respondents, 53.8% demonstrated high knowledge of monkeypox. People aged 18–25 years, unmarried people, and those living in urban areas had significantly higher levels of knowledge. Most respondents believed that MPXV is not a conspiracy or bioterrorism (63.1%) and agreed that it is likely to affect people’s social and economic life as COVID-19 did (67.0%). The history of COVID-19 vaccination (aOR: 2.980; 95%CI: 1.227, 7.236) and the younger age (aOR: 2.975; 95%CI: 1.097, 8.069) were found to be significant determinants of the knowledge of the participants on monkeypox.

Conclusion

We observed that most Nepalese populations had a high knowledge of monkeypox and that social media was the most valuable source of information.
Polycystic intrahepatic infection caused by Enterococcus casseliflavus: a case report and literature review
Authors: Xu Senyin , Huang Bin , Cao Youjun , Zhong Zhongyong , Yin Jiazhen
Published: March 2024

Background

Enterococcus casseliflavus is a rare pathogenic bacterium that is characterized by vancomycin resistance and can lead to multiple infections in the human body. This report describes a rare case of polycystic intrahepatic infection with E. casseliflavus which necessitated antibiotic treatment and surgical intervention involving cystic drainage.

Case Presentation

A 59-year-old woman, a long-term hemodialysis patient, was hospitalized due to a 5-day history of fever, abdominal pain, and diarrhea, which were possibly caused by the ingestion of contaminated food. Her blood culture yielded a positive result for E. casseliflavus, and she was initially treated with piperacillin/tazobactam and linezolid. Later, the antibiotic regimen was adjusted to include meropenem and linezolid. Despite treatment, her body temperature remained elevated. However, subsequent blood cultures were negative for E.casseliflavus.Conventional CT scans and ultrasound examinations did not identify the source of infection. However, a PET-CT examination indicated an intrahepatic cyst infection. Following MRI and ultrasound localization, percutaneous intrahepatic puncture and drainage were performed on the 20th day. Fluoroquinolones were administered for 48 days. On the 32nd day, MRI revealed a separation within the infected cyst, leading to a repeat percutaneous drainage at a different site. Subsequently, the patient’s temperature returned to normal. The infection was considered resolved, and she was discharged on the 62nd day. Follow-up results have been favorable thus far.

Conclusions

Based on the findings from this case, it is recommended to promptly conduct PET-CT examination to exclude the possibility of intracystic infection in cases of polycystic liver infection that are challenging to control. Furthermore, timely consideration should be given to puncture drainage in difficult cases.
Neuroanatomical and neurocognitive correlates of delusion in Alzheimer’s disease and mild cognitive impairment
Authors: Kwak Seyul , Kim Hairin , Kim Keun You , Oh Da Young , Lee Dasom , Nam Gieun , Lee Jun-Young
Published: March 2024

Background

Neuropsychiatric symptoms and delusions are highly prevalent among people with dementia. However, multiple roots of neurobiological bases and shared neural basis of delusion and cognitive function remain to be characterized. By utilizing a fine-grained multivariable approach, we investigated distinct neuroanatomical correlates of delusion symptoms across a large population of dementing illnesses.

Methods

In this study, 750 older adults with mild cognitive impairment and Alzheimer’s disease completed brain structural imaging and neuropsychological assessment. We utilized principal component analysis followed by varimax rotation to identify the distinct multivariate correlates of cortical thinning patterns. Five of the cognitive domains were assessed whether the general cognitive abilities mediate the association between cortical thickness and delusion.

Results

The result showed that distributed thickness patterns of temporal and ventral insular cortex (component 2), inferior and lateral prefrontal cortex (component 1), and somatosensory-visual cortex (component 5) showed negative correlations with delusions. Subsequent mediation analysis showed that component 1 and 2, which comprises inferior frontal, anterior insula, and superior temporal regional thickness accounted for delusion largely through lower cognitive functions. Specifically, executive control function assessed with the Trail Making Test mediated the relationship between two cortical thickness patterns and delusions.

Discussion

Our findings suggest that multiple distinct subsets of brain regions underlie the delusions among older adults with cognitive impairment. Moreover, a neural loss may affect the occurrence of delusion in dementia largely due to impaired general cognitive abilities.
Perinatal depression trajectories and child development at one year: a study in China
Authors: Zhu Yuan , Li Xiaoyu , Chen Junyu , Gong Wenjie
Published: March 2024

Background

The objective of the current study was to investigate the correlation between trajectories of maternal perinatal depression (PND) spanning from early pregnancy to one year postpartum and developmental delays observed in one-year-old children.

Methods

The dataset under examination encompassed 880 women who took part in a mother-child birth study conducted in China. Latent class growth analysis (LCGA) was employed to identify patterns in Edinburgh Postnatal Depression Scale (EPDS) scores of women, spanning from early pregnancy to one year postpartum. To assess the neurodevelopment of one-year-old children, a Chinese version of the Bayley Scale of Infant Development (BSID-CR) was employed. Logistic regression was employed to explore the association between PND trajectories and developmental delays in children, with appropriate covariate adjustments.

Results

The trajectories of maternal PND identified in this study included a minimal-stable symptom group (n = 155), low-stable symptom group (n = 411), mild-stable symptom group (n = 251), and moderate-stable symptom group (n = 63). Logistic regression analysis revealed that mothers falling into the moderate-stable symptom group exhibited a notably heightened risk of having a child with psychomotor developmental delays at the age of one year.

Conclusions

The findings drawn from a representative sample in China provide compelling empirical evidence that bolsters the association between maternal PND and the probability of psychomotor developmental delays in children. It is imperative to develop tailored intervention strategies and meticulously design mother-infant interactive intervention programs for women with PND.