bluuebunny/crossref_metadata_2025 huggingface dataset:
High Temperature Oxidation and Thermal Shock Properties of La2Zr2O7 Thermal Barrier Coatings Deposited on Nickel-Based Superalloy by Laser-Cladding
Authors: Huang Kaijin , Li Wei , Pan Kai , Lin Xin , Wang Aihua
Published: April 2020

In order to reduce the difficulty and cost of manufacturing and improve the high temperature oxidation and thermal shock properties of nickel-based superalloy, a thin La2Zr2O7 thermal barrier coating without bond coat was successfully prepared by laser-cladding using La2Zr2O7 powders on a nickel-based superalloy substrate. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) methods were used to characterize the microstructure of the coating. The high temperature oxidation and thermal shock properties of the coating were evaluated by the air isothermal oxidation method at 1100 °C for 110 h and thermal cycling method at 25~1100 °C, respectively. The results show that the coating is mainly composed of La2Zr2O7 phase. The oxidation weight gain rate of the coating is about two-thirds of that of the substrate, and the first crack thermal shock lifetime of the coating is about 1.67 times of that of the substrate. The oxidation products of the coating are mainly Fe2O3, Cr2O3, NiCr2O4, Nb2O5 and La2Zr2O7. The existence of La2Zr2O7 phase in the coating is the main reason for the improvement of its oxidation resistance at 1100 °C and its thermal shock resistance at 25~1100 °C.
The Effects of Doping on the Electronic Characteristics and Adsorption Behavior of Silicon Polyprismanes
Authors: Grishakov Konstantin , Katin Konstantin , Maslov Mikhail
Published: April 2020

Quantum–chemical calculations of the electronic characteristics of carbon and boron-doped silicon polyprismanes were carried out, and the atomic hydrogen adsorption on these structures was analyzed. It was established that silicon polyprismanes doped with boron and carbon retained their metallicity predicted earlier. It was shown that the doping of polyprismanes made them more thermodynamically stable. For the silicon prismanes doped with boron or carbon, hydrogen adsorption was found to be energetically favorable. In the case of boron-doped prismanes, adsorption on the boron impurity was much more advantageous than on the neighboring silicon nodes. For the carbon doping, the adsorption energy of polyprismane with a small diameter weakly depended on the position of the hydrogen atom near the impurity center. However, for the C-doped polyprismanes with a larger diameter, the hydrogen adsorption on the silicon atom belonging to the ring with impurity is more energetically favorable than the adsorption on the silicon atom from the adjacent ring.
One-Pot Synthesis, X-ray Single Crystal and Molecular Insight of Enaminone-Based β-Morpholino-/N-Methylpiperazinyl-/Pyrrolidinylpropiophenone
Authors: Barakat Assem , Soliman Saied M. , Haukka Matti , Al-Majid Abdullah Mohammed , Islam Mohammad Shahidul , Ali M. , Shaik Mohammed Rafi
Published: April 2020

One-pot synthesis of three enaminones, (E)-1-(4-chlorophenyl)-3-morpholinoprop-2-en-1-one 1, (E)-1-(4-chlorophenyl)-3-(4-methylpiperazin-1-yl)prop-2-en-1-one 2, and (E)-1-(4-chlorophenyl)-3-(pyrrolidin-1-yl)prop-2-en-1-one 3 were achieved. The synthetic protocol via three components reaction of p-chloroacetophenone with DMFDMA (N,N-dimethylformamid-dimethylacetal) and the corresponding secondary amines (morpholine/N-methylpiperazine/pyrrolidine) in dioxane under heating for 2.5–4 h at 102 °C yielded the requisite enaminones. This protocol has the advantage of no separation of intermediate, no need for column purification with quantitative yield for the target compounds. The chemical features of the β-enaminones 1–3 were assigned by NMR. β-Enaminones 1, and 2 were assigned by single crystal X-ray diffraction technique. The intermolecular interactions in the crystal structures were analyzed quantitatively using Hirshfeld analysis. The Cl…H and O…H hydrogen bonds are common in both compounds while the C-H…π and N…H contacts are more significant in 2 than 1. DFT studies were investigated to show the electronic and spectroscopic properties (NMR and UV-Vis) of the studied systems.
Monitoring Selective Logging in a Pine-Dominated Forest in Central Germany with Repeated Drone Flights Utilizing A Low Cost RTK Quadcopter
Authors: Thiel Christian , Müller Marlin M. , Berger Christian , Cremer Felix , Dubois Clémence , Hese Sören , Baade Jussi , Klan Friederike , Pathe Carsten
Published: April 2020

There is no doubt that unmanned aerial systems (UAS) will play an increasing role in Earth observation in the near future. The field of application is very broad and includes aspects of environmental monitoring, security, humanitarian aid, or engineering. In particular, drones with camera systems are already widely used. The capability to compute ultra-high-resolution orthomosaics and three-dimensional (3D) point clouds from UAS imagery generates a wide interest in such systems, not only in the science community, but also in industry and agencies. In particular, forestry sciences benefit from ultra-high-structural and spectral information as regular tree level-based monitoring becomes feasible. There is a great need for this kind of information as, for example, due to the spring and summer droughts in Europe in the years 2018/2019, large quantities of individual trees were damaged or even died. This study focuses on selective logging at the level of individual trees using repeated drone flights. Using the new generation of UAS, which allows for sub-decimeter-level positioning accuracies, a change detection approach based on bi-temporal UAS acquisitions was implemented. In comparison to conventional UAS, the effort of implementing repeated drone flights in the field was low, because no ground control points needed to be surveyed. As shown in this study, the geometrical offset between the two collected datasets was below 10 cm across the site, which enabled a direct comparison of both datasets without the need for post-processing (e.g., image matching). For the detection of logged trees, we utilized the spectral and height differences between both acquisitions. For their delineation, an object-based approach was employed, which was proven to be highly accurate (precision = 97.5%; recall = 91.6%). Due to the ease of use of such new generation, off-the-shelf consumer drones, their decreasing purchase costs, the quality of available workflows for data processing, and the convincing results presented here, UAS-based data can and should complement conventional forest inventory practices.
Presence of Root Rot Reduces Stability of Norway Spruce (Picea abies): Results of Static Pulling Tests in Latvia
Authors: Krisans Oskars , Matisons Roberts , Rust Steffen , Burnevica Natalija , Bruna Lauma , Elferts Didzis , Kalvane Liene , Jansons Aris
Published: April 2020

Storms are the main abiotic disturbance in European forests, effects of which are expected to intensify in the future, hence the importance of forest stand stability is increasing. The predisposition of Norway spruce to wind damage appears to be enhanced by pathogens such as Heterobasidion spp., which reduce stability of individual trees. However, detailed information about the effects of the root rot on the stability of individual trees across diverse soil types is still lacking. The aim of the study was to assess the effect of root rot on the individual tree stability of Norway spruce growing on drained peat and mineral soils. In total, 77 Norway spruce trees (age 50–80 years) growing in four stands were tested under static loading. The presence of Heterobasidion spp. had a significant negative effect on the bending moment at primary and secondary failure of the tested trees irrespectively of soil type. This suggests increased legacy effects (e.g., susceptibility to pathogens and pests due to fractured roots and altered water uptake) of storms. Damaged trees act as weak spots increasing the susceptibility of stands to wind damage, thus forming a negative feedback loop and contributing to an ongoing decline in vitality of Norway spruce stands following storms in the study region in the future. Accordingly, the results support the importance of timely identification of the decayed trees, lowering stand density and/or shortening rotation period as the measures to counteract the increasing effects of storms on Norway spruce stands.
Assessment of Bending Properties of Sawn and Glulam Blackwood in Portugal
Authors: Martins Carlos , Monteiro Sandra , Knapic Sofia , Dias Alfredo
Published: April 2020

Portuguese forests have changed in recent years. These changes were mainly boosted by the wildfires that affected a significant percentage of the softwood area. Data from 2015, conveyed by the Portuguese Institute for Nature Conservation and Forests, indicates that hardwoods occupy 70% of the Portuguese forest area. This paper presents the Blackwood (Acacia melanoxylon R. Br.) species potential, focusing on construction applications, based on recent studies performed at the University of Coimbra and SerQ—Forest Innovation and Competences Center. The valuation of Blackwood for structural applications has been considered through the non-destructive and destructive assessment of their mechanical properties as sawn wood. Their potential was also assessed for a more technologically engineered wood product, the glulam. The dynamic modulus of elasticity (MOE) was estimated through the Longitudinal Vibration Method (LVM) and the Transformed Section Method (TSM); the static MOE and bending strength were assessed through a four-point bending test. Agreement was obtained between both approaches. Sawn Portuguese Blackwood showed a density of 647 kg/m3, 13,900 MPa of MOE and a bending strength of 65 MPa (mean values). The glulam beams fabricated with this raw material had improved properties relative to sawn wood, most obviously concerning the bending strength, with an improvement of 29%. This proves the significant ability and potential of these species to be used in construction products with structural purposes like sawn wood and glulam.
Individual Tree Diameter and Height Growth Models for 30 Tree Species in Mixed-Species and Uneven-Aged Forests of Mexico
Authors: Briseño-Reyes Jaime , Corral-Rivas José Javier , Solis-Moreno Raúl , Padilla-Martínez Jaime Roberto , Vega-Nieva Daniel José , López-Serrano Pablito Marcelo , Vargas-Larreta Benedicto , Diéguez-Aranda Ulises , Quiñonez-Barraza Gerónimo , López-Sánchez Carlos Antonio
Published: April 2020

Lack of knowledge of individual tree growth in species-rich, mixed forest ecosystems impedes their sustainable management. In this study, species-specific models for predicting individual diameter at breast height (dbh) and total tree height (h) growth were developed for 30 tree species growing in mixed and uneven-aged forest stands in Durango, Mexico. Growth models were also developed for all pine, all oaks, and all other species of the genus Arbutus (strawberry trees). A database of 55,158 trees with remeasurements of dbh and h of a 5-year growth period was used to develop the models. The data were collected from 217 stem-mapped plots located in the Sierra Madre Occidental (Mexico). Weighted regression was used to remove heteroscedasticity from the species-specific dbh and h growth models using a power function of the tree size independent variables. The final models developed in the present study to predict dbh and total tree height growth included size variables, site factors, and competition variables in their formulation. The developed models fitted the data well and explained between 98 and 99% and of the observed variation of dbh, and between 77 and 98% of the observed variation of total tree height for the studied species and groups of species. The developed models can be used for estimating the individual dbh and h growth for the analyzed species and can be integrated in decision support tools for management planning in these mixed forest ecosystems.
Physicochemical Properties and Resistant Starch Content of Corn Tortilla Flours Refrigerated at Different Storage Times
Authors: Rojas-Molina Isela , Mendoza-Avila Monsserrat , Cornejo-Villegas María de los Ángeles , Real-López Alicia Del , Rivera-Muñoz Eric , Rodríguez-García Mario , Gutiérrez-Cortez Elsa
Published: April 2020

The tortilla is a foodstuff that has a short shelf-life, causing great losses to the industry. The objective of this work was to evaluate, for the first time, the physicochemical properties and resistant starch (RS) content of flours. These were obtained from nixtamalized corn tortillas made with traditional and industrial (commercial) methods, stored at 4 °C for 7, 15, and 30 days. The flours were characterized by measuring particle size distribution, color, water absorption index (WAI), water solubility index (WSI), viscosity, calcium, and RS content. Additionally, chemical proximate analysis, scanning electron microscopy (SEM), and thermal analysis were conducted. Storage at 4 °C increased the friability of tortillas and shifted the particle size distribution toward a greater content of coarse particles in corn tortilla flours. The commercial corn tortilla flours showed higher WAI and WSI values than the traditional corn tortilla flours. On the other hand, the traditional corn tortilla flours exhibited higher RS content values than commercial corn tortilla flours as well as peak viscosity. X-ray diffractograms revealed the presence of amylose-lipid complexes (RS5) in experimental samples. The thermograms evidenced three endotherms corresponding to corn starch gelatinization and melting of type I and type II amylose–lipid complexes.
High-Throughput Sequencing Identifies 3 Novel Susceptibility Genes for Hereditary Melanoma
Authors: Campos Catarina , Fragoso Sofia , Luís Rafael , Pinto Filipe , Brito Cheila , Esteves Susana , Pataco Margarida , Santos Sidónia , Machado Patrícia , Vicente João B. , Rosa Joaninha Costa , Cavaco Branca M. , Moura Cecília , Pojo Marta
Published: April 2020

Cutaneous melanoma is one of the most aggressive human cancers due to its high invasiveness. Germline mutations in high-risk melanoma susceptibility genes have been associated with development hereditary melanoma; however, most genetic culprits remain elusive. To unravel novel susceptibility genes for hereditary melanoma, we performed whole exome sequencing (WES) on eight patients with multiple primary melanomas, high number of nevi, and negative for high and intermediate-risk germline mutations. Thirteen new potentially pathogenic variants were identified after bioinformatics analysis and validation. CDH23, ARHGEF40, and BRD9 were identified as the most promising susceptibility genes in hereditary melanoma. In silico analysis of CDH23 and ARHGEF40 variants provided clues for altered protein structure and function associated with the identified mutations. Then, we also evaluated the clinical value of CDH23, ARHGEF40, and BRD9 expression in sporadic melanoma by using the TCGA dataset (n = 461). No differences were observed in BRD9 expression between melanoma and normal skin samples, nor with melanoma stage, whereas ARHGEF40 was found overexpressed, and CDH23 was downregulated and its loss was associated with worse survival. Altogether, these results reveal three novel genes with clinical relevance in hereditary and sporadic melanoma.
A New Census of Protein Tandem Repeats and Their Relationship with Intrinsic Disorder
Authors: Delucchi Matteo , Schaper Elke , Sachenkova Oxana , Elofsson Arne , Anisimova Maria
Published: April 2020

Protein tandem repeats (TRs) are often associated with immunity-related functions and diseases. Since that last census of protein TRs in 1999, the number of curated proteins increased more than seven-fold and new TR prediction methods were published. TRs appear to be enriched with intrinsic disorder and vice versa. The significance and the biological reasons for this association are unknown. Here, we characterize protein TRs across all kingdoms of life and their overlap with intrinsic disorder in unprecedented detail. Using state-of-the-art prediction methods, we estimate that 50.9% of proteins contain at least one TR, often located at the sequence flanks. Positive linear correlation between the proportion of TRs and the protein length was observed universally, with Eukaryotes in general having more TRs, but when the difference in length is taken into account the difference is quite small. TRs were enriched with disorder-promoting amino acids and were inside intrinsically disordered regions. Many such TRs were homorepeats. Our results support that TRs mostly originate by duplication and are involved in essential functions such as transcription processes, structural organization, electron transport and iron-binding. In viruses, TRs are found in proteins essential for virulence.
Gallop Racing Shifts Mature mRNA towards Introns: Does Exercise-Induced Stress Enhance Genome Plasticity?
Authors: Cappelli Katia , Mecocci Samanta , Gioiosa Silvia , Giontella Andrea , Silvestrelli Maurizio , Cherchi Raffaele , Valentini Alessio , Chillemi Giovanni , Capomaccio Stefano
Published: April 2020

Physical exercise is universally recognized as stressful. Among the “sport species”, the horse is probably the most appropriate model for investigating the genomic response to stress due to the homogeneity of its genetic background. The aim of this work is to dissect the whole transcription modulation in Peripheral Blood Mononuclear Cells (PBMCs) after exercise with a time course framework focusing on unexplored regions related to introns and intergenic portions. PBMCs NGS from five 3 year old Sardinian Anglo-Arab racehorses collected at rest and after a 2000 m race was performed. Apart from differential gene expression ascertainment between the two time points the complexity of transcription for alternative transcripts was identified. Interestingly, we noted a transcription shift from the coding to the non-coding regions. We further investigated the possible causes of this phenomenon focusing on genomic repeats, using a differential expression approach and finding a strong general up-regulation of repetitive elements such as LINE. Since their modulation is also associated with the “exonization”, the recruitment of repeats that act with regulatory functions, suggesting that there might be an active regulation of this transcriptional shift. Thanks to an innovative bioinformatic approach, our study could represent a model for the transcriptomic investigation of stress.
A Mixed-Valence Tetra-Nuclear Nickel Dithiolene Complex: Synthesis, Crystal Structure, and the Lability of Its Nickel Sulfur Bonds
Authors: Ahmadi Mohsen , Correia Jevy , Chrysochos Nicolas , Schulzke Carola
Published: April 2020

In this study, by employing a common synthetic protocol, an unusual and unexpected tetra-nuclear nickel dithiolene complex was obtained. The synthesis of the [Ni4(ecpdt)6]2− dianion (ecpdt = (Z)-3-ethoxy-3-oxo-1-phenylprop-1-ene-1,2-bis-thiolate) with two K+ as counter ions was then intentionally reproduced. The formation of this specific complex is attributed to the distinct dithiolene precursor used and the combination with the then coordinated counter ion in the molecular solid-state structure, as determined by X-ray diffraction. K2[Ni4(ecpdt)6] was further characterized by ESI-MS, FT-IR, UV-Vis, and cyclic voltammetry. The tetra-nuclear complex was found to have an uncommon geometry arising from the combination of four nickel centers and six dithiolene ligands. In the center of the arrangement, suspiciously long Ni–S distances were found, suggesting that the tetrameric structure can be easily split into two identical dimeric fragments or two distinct groups of monomeric fragments, for instance, upon dissolving. A proposed variable magnetism in the solid-state and in solution due to the postulated dissociation was confirmed. The Ni–S bonds of the “inner” and “outer” nickel centers differed concurrently with their coordination geometries. This observation also correlates with the fact that the complex bears two anionic charges requiring the four nickel centers to be present in two distinct oxidation states (2 × +2 and 2 × +3), i.e., to be hetero-valent. The different coordination geometries observed, together with the magnetic investigation, allowed the square planar “outer” geometry to be assigned to d8 centers, i.e., Ni2+, while the Ni3+ centers (d7) were in a square pyramidal geometry with longer Ni–S distances due to the increased number of donor atoms and interactions.
Numerical Investigations of Cavitation Nose Structure of a High-Speed Projectile Impact on Water-Entry Characteristics
Authors: Li Qiang , Lu Lin
Published: April 2020

In this study, a detailed analysis of the influences of cavitation nose structure of a high-speed projectile on the trajectory stability during the water-entry process was investigated numerically. The Zwart-Gerber-Belamri (Z-G-B) cavitation model and the Shear Stress Ttransport (SST)k-ω turbulence model based on the Reynolds Averaged Navier–Stokes (RANS) method were employed. The numerical methodology was validated by comparing the numerical simulation results with the experimental photograph of cavitation shape and the experimental underwater velocity. Based on the numerical methodology, the disk and the conical cavitation noses were selected to investigate the water-entry characteristics. The influences of cavitation nose angle and cavitation nose diameter of the projectile on the trajectory stability and flow characteristics were carried out in detail. The variation features of projectile trajectory, velocity attenuation and drag were conducted, respectively. In addition, the cavitation characteristics of water-entry is presented and analyzed. Results show that the trajectory stability can be improved by increasing the cavitation nose angle, but the drag reduction performance will be reduced simultaneously. Additionally, due to the weakening of drag reduction performance, the lower velocity of the projectile will cause the damage of the cavitation shape and the trajectory instability. Furthermore, the conical cavitation nose has preferable trajectory stability and drag reduction performance than the disk cavitation nose.
Cross-Flow Microfiltration of Glycerol Fermentation Broths with Citrobacter freundii
Authors: Tomczak Wirginia , Gryta Marek
Published: April 2020

This paper reports the study of the cross-flow microfiltration (MF) of glycerol fermentation broths with Citrobacter freundii bacteria. A single channel tubular ceramic membrane with a nominal pore size of 0.14 µm was used. It has been demonstrated that the MF ceramic membrane has been successfully applied to bacteria cell removal and to effectively eliminate colloidal particles from glycerol fermentation broths. However, due to fouling, the significant reduction of the MF performance has been demonstrated. In order to investigate the impact of transmembrane pressure (TMP) and feed flow rate (Q) on MF performance, 24 experiments have been performed. The highest steady state permeate flux (138.97 dm3/m2h) was achieved for 0.12 MPa and 1000 dm3/h. Fouling analysis has been studied based on the resistance-in series model. It has been found that the percentage of irreversible fouling resistance during the MF increases with increasing TMP and Q. The permeate flux regeneration has been achieved by membrane cleaning with 3 wt % NaOH and 3 wt % H3PO4 at 45 °C. The results of this study are expected to be useful in industrially employing the MF process as the first step of glycerol fermentation broth purification.
Unravelling the Role of Melanin in Cd and Zn Tolerance and Accumulation of Three Dark Septate Endophytic Species
Authors: Berthelot Charlotte , Zegeye Asfaw , Gaber Dalia A. , Chalot Michel , Franken Philipp , Kovács Gábor M. , Leyval Corinne , Blaudez Damien
Published: April 2020

Dark septate endophytes (DSEs) are often trace element (TE)-tolerant fungi and are abundant in TE-polluted environments. The production of melanin, a black polymer found in cell walls, was hypothesized by several authors to play a role in the TE tolerance of DSEs. To test this hypothesis, we established a series of experiments using albino strains and melanin inhibitors and examined the responses to Cd and Zn. Six DSEs belonging to genera Cadophora sp., Leptodontidium sp. and Phialophora mustea, were evaluated. The strains mainly produced 1,8-dihydroxynaphthalene (DHN) melanin whereas 3,4-dihydroxyphenylalanin melanin was also synthetized. Cd and Zn decreased melanin synthesis in most of the strains. A reduction in melanin concentration in hyphae through the use of tricyclazole, an inhibitor of DHN-melanin synthesis, did not reduce the tolerance of the strains to Cd and Zn. Similarly, albino mutants of Leptodontidium sp. were not more sensitive to Cd and Zn than the WT strain. Moreover, tricyclazole-treated colonies accumulated less Cd but more Zn compared to untreated colonies. The Cd and Zn contents of Leptodontidium albino strains were variable and similar to that of the WT. The results suggest that melanin production is not an important functional trait that contributes to Cd and Zn tolerance, but might contribute to Cd accumulation.
Diminished Pathogen and Enhanced Endophyte Colonization upon CoInoculation of Endophytic and Pathogenic Fusarium Strains
Authors: Constantin Maria E. , Vlieger Babette V. , Takken Frank L. W. , Rep Martijn
Published: April 2020

Root colonization by Fusarium oxysporum (Fo) endophytes reduces wilt disease symptoms caused by pathogenic Fo strains. The endophytic strain Fo47, isolated from wilt suppressive soils, reduces Fusarium wilt in various crop species such as tomato, flax, and asparagus. How endophyte-mediated resistance (EMR) against Fusarium wilt is achieved is unclear. Here, nonpathogenic colonization by Fo47 and pathogenic colonization by Fo f.sp. lycopersici (Fol) strains were assessed in tomato roots and stems when inoculated separately or coinoculated. It is shown that Fo47 reduces Fol colonization in stems of both noncultivated and cultivated tomato species. Conversely, Fo47 colonization of coinoculated tomato stems was increased compared to single inoculated plants. Quantitative PCR of fungal colonization of roots (co)inoculated with Fo47 and/or Fol showed that pathogen colonization was drastically reduced when coinoculated with Fo47, compared with single inoculated roots. Endophytic colonization of tomato roots remained unchanged upon coinoculation with Fol. In conclusion, EMR against Fusarium wilt is correlated with a reduction of root and stem colonization by the pathogen. In addition, the endophyte may take advantage of the pathogen-induced suppression of plant defences as it colonizes tomato stems more extensively.
The Robustness of the Gray Level Co-Occurrence Matrices and X-Ray Computed Tomography Method for the Quantification of 3D Mineral Texture
Authors: Voigt Marcelene , Miller Jodie A. , Mainza Aubrey N. , Bam Lunga C. , Becker Megan
Published: April 2020

Mineral textural quantification methods have become critical in both geosciences and mineral processing as mineral texture is a critical factor contributing to ore variability. However, the lack of objective mineral texture classification has made quantification difficult. The aim of this study is therefore to investigate the robustness of applying the gray level co-occurrence matrices (GLCM) to 3-dimensional (3D) gray scale images measured by X-ray computed tomography (XCT) for the quantification of mineral texture in 3D. The data quality of the GLCM outputs like statistics, heat maps and histograms in response to changes in XCT conditions such as artefacts, resolution, and calibration was tested. The response of the GLCM outputs with respect to different mineral texture types with anisotropic features and inter-sample variability was also explored. The methodology included testing core sizes of 26, 19, 14, and 6 mm diameter. Calibration was tested using copper and tungsten wires. The study demonstrated the versatility of the method for different sample types. Inter-sample calibration and optimal scanning conditions (quality and integrity) were also demonstrated, and a basic link between the 3D GLCM statistical descriptors with the mineral texture features of rocks was established. The 3D mineral texture method can potentially bypass the XCT segmentation process for direct automation of 3D mineral texture information.
Improving Children’s E-Safety Skills through an Interactive Learning Environment: A Quasi-Experimental Study
Authors: Nicolaidou Iolie , Venizelou Agnes
Published: April 2020

There is a worldwide concern for young children’s online safety and a growing necessity for e-safety skills to be taught to children from a young age as part of formal schooling. The purpose of this study was to design and evaluate the effectiveness and motivational capacity of an interactive web-based learning environment for improving children’s e-safety skills. A quasi-experimental pre-test post-test control group design was used with an experimental group of 48 sixth-grade primary school students, who used the web-based learning environment over two 80-min lessons, and a control group of 25 students who did not. Findings revealed a statistically significant difference (t(47) = −14.06, p < 0.01) in the experimental group students’ e-safety performance, when students’ pre-test scores (mean (Μ) = 41.13, SD = 10.47) were compared to their post-test scores (Μ = 56.69, SD = 9.38). The analysis of an attitudes questionnaire and of student interviews documented the experimental group students’ positive attitudes toward the learning environment. Findings provide evidence of the effectiveness and motivational capacity of the web-based learning environment, which can be used in either formal education or informal learning settings, for improving children’s e-safety skills.
Advances in Nickel Nanoparticle Synthesis via Oleylamine Route
Authors: Heilmann Maria , Kulla Hannes , Prinz Carsten , Bienert Ralf , Reinholz Uwe , Guilherme Buzanich Ana , Emmerling Franziska
Published: April 2020

Nickel nanoparticles are an active research area due to their multiple applications as catalysts in different processes. A variety of preparation techniques have been reported for the synthesis of these nanoparticles, including solvothermal, microwave-assisted, and emulsion techniques. The well-studied solvothermal oleylamine synthesis route comes with the drawback of needing standard air-free techniques and often space-consuming glassware. Here, we present a facile and straightforward synthesis method for size-controlled highly monodisperse nickel nanoparticles avoiding the use of, e.g., Schlenk techniques and space-consuming labware. The nanoparticles produced by this novel synthetic route were investigated using small-angle X-ray scattering, transmission electron microscopy, X-ray diffraction, and X-ray spectroscopy. The nanoparticles were in a size range of 4–16 nm, show high sphericity, no oxidation, and no agglomeration after synthesis.
Photophysical Properties of Multilayer Graphene–Quantum Dots Hybrid Structures
Authors: Reznik Ivan , Zlatov Andrey , Baranov Mikhail , Zakoldaev Roman , Veniaminov Andrey , Moshkalev Stanislav , Orlova Anna
Published: April 2020

Photoelectrical and photoluminescent properties of multilayer graphene (MLG)–quantum dots (QD) hybrid structures have been studied. It has been shown that the average rate of transfer from QDs to the MLG can be estimated via photoinduced processes on the QDs’ surfaces. A monolayer of CdSe QDs can double the photoresponse amplitude of multilayer graphene, without influencing its characteristic photoresponse time. It has been found that efficient charge or energy transfer from QDs to MLG with a rate higher than 3 × 108 s−1 strongly inhibits photoinduced processes on the QD surfaces and provides photostability for QD-based structures.
Regulatory and Policy-Related Aspects of Calcium Fortification of Foods. Implications for Implementing National Strategies of Calcium Fortification
Authors: Cormick Gabriela , Betrán Ana Pilar , Metz Fletcher , Palacios Cristina , Beltrán-Velazquez Filiberto , García-Casal María de las Nieves , Peña-Rosas Juan Pablo , Hofmeyr G. Justus , Belizán José M.
Published: April 2020

Daily calcium intake is well below current recommendations in most low- and middle-income countries (LMICs). Calcium intake is usually related to bone health, however an adequate calcium intake has also been shown to reduce hypertensive disorders of pregnancy, lower blood pressure and cholesterol values, and to prevent recurrent colorectal adenomas. Fortification of foods has been identified as a cost-effective strategy to overcome micronutrient gaps in public health. This review summarizes regulatory aspects of fortification of commonly consumed foods with micronutrients, with an emphasis on calcium. We selected a convenient sample of 15 countries from different WHO regions and described the regulatory framework related to calcium fortification of staple foods. We assessed the relevant policies in electronic databases including the WHO Global database on the Implementation of Nutrition Action (GINA) for fortification policies and the Global Fortification Data Exchange Database, a fortification database developed and maintained by Food Fortification Initiative (FFI), Global Alliance for Improved Nutrition (GAIN), Iodine Global Network (IGN), and Micronutrient Forum. Food fortification with micronutrients is widely used in many of the selected countries. Most countries had national legislation for the addition of micronutrients to staple foods, especially wheat flour. These national legislations, that includes regulations and standards, can provide the framework to consider the implementation of adding calcium to the fortification strategies, including the selection of the adequate food vehicle to reach the targeted population at risk safely. The strategy to include calcium in the fortification mix in fortified staple foods seems promising in these countries. However, potential undesired changes on the organoleptic characteristics of fortified foods and products thereof, and operational feasibility at the manufacturing site should be evaluated by the stakeholders during the planning stage. Codex Alimentarius standards should be considered by regulators in order to assure adherence to international standards. While the selected countries already have established national regulations and/or standards for fortification of key staple food vehicles, and there are experiences in the implementation of fortification of some staple foods, national food intake surveys can help plan, design, and modify existing fortification programs as well as monitor food and nutrient consumption to assess risk and benefits.
Breast Milk, a Source of Beneficial Microbes and Associated Benefits for Infant Health
Authors: Lyons Katríona E. , Ryan C. Anthony , Dempsey Eugene M. , Ross R. Paul , Stanton Catherine
Published: April 2020

Human breast milk is considered the optimum feeding regime for newborn infants due to its ability to provide complete nutrition and many bioactive health factors. Breast feeding is associated with improved infant health and immune development, less incidences of gastrointestinal disease and lower mortality rates than formula fed infants. As well as providing fundamental nutrients to the growing infant, breast milk is a source of commensal bacteria which further enhance infant health by preventing pathogen adhesion and promoting gut colonisation of beneficial microbes. While breast milk was initially considered a sterile fluid and microbes isolated were considered contaminants, it is now widely accepted that breast milk is home to its own unique microbiome. The origins of bacteria in breast milk have been subject to much debate, however, the possibility of an entero-mammary pathway allowing for transfer of microbes from maternal gut to the mammary gland is one potential pathway. Human milk derived strains can be regarded as potential probiotics; therefore, many studies have focused on isolating strains from milk for subsequent use in infant health and nutrition markets. This review aims to discuss mammary gland development in preparation for lactation as well as explore the microbial composition and origins of the human milk microbiota with a focus on probiotic development.
Exposure-Response and Clinical Outcome Modeling of Inhaled Budesonide/Formoterol Combination in Asthma Patients
Authors: Back Hyun-moon , Lee Jong Bong , Kim Anhye , Park Seon-Jong , Kim Junyeong , Chae Jung-woo , Sheen Seung Soo , Kagan Leonid , Park Hae-Sim , Ye Young-Min , Yun Hwi-yeol
Published: April 2020

Exposure-response and clinical outcome (CO) model for inhaled budesonide/formoterol was developed to quantify the relationship among pharmacokinetics (PK), pharmacodynamics (PD) and CO of the drugs and evaluate the covariate effect on model parameters. Sputum eosinophils cationic proteins (ECP) and forced expiratory volume (FEV1) were selected as PD markers and asthma control score was used as a clinical outcome. One- and two-compartment models were used to describe the PK of budesonide and formoterol, respectively. The indirect response model (IDR) was used to describe the PD effect for ECP and FEV1. In addition, the symptomatic effect on the disease progression model for CO was connected with IDR on each PD response. The slope for the effect of ECP and FEV1 to disease progression were estimated as 0.00008 and 0.644, respectively. Total five covariates (ex. ADRB2 genotype etc.) were searched using a stepwise covariate modeling method, however, there was no significant covariate effect. The results from the simulation study were showed that a 1 puff b.i.d. had a comparable effect of asthma control with a 2 puff b.i.d. As a result, the 1 puff b.i.d. of combination drug could be suggested as a standardized dose to minimize the side effects and obtain desired control of disease compared to the 2 puff b.i.d.
Cytochrome P450 (CYP450) Interactions Involving Atypical Antipsychotics Are Common in Community-Dwelling Older Adults Treated for Behavioral and Psychological Symptoms of Dementia
Authors: Matos Adriana , Bain Kevin T. , Bankes David L. , Furman Anna , Skalski Briana , Verzicco James , Turgeon Jacques
Published: April 2020

Treatment of behavioral and psychological symptoms of dementia (BPSD) and comorbidities often necessitates the concomitant use of antipsychotics and non-antipsychotic drugs, thereby potentiating the risk for drug–drug interactions (DDIs). The primary objective of our study was to identify potentially clinically relevant cytochrome P450 (CYP)-mediated DDIs involving antipsychotics among participants enrolled in the Program of All-Inclusive Care for the Elderly (PACE) with BPSD. Additionally, we wanted to determine the prevalence of antipsychotic use in this population. The study included 10,001 PACE participants. The practice setting used a proprietary clinical decision support system (CDSS) to analyze simultaneous multidrug interactions. A retrospective analysis of pharmacy claims data was conducted to identify DDIs involving antipsychotics prescribed for BPSD, using snapshots of medication profiles paired with the CDSS. Of the participants who met inclusion criteria, 1190 (11.9%) were prescribed an antipsychotic; of those, 1071 (90.0%) were prescribed an atypical antipsychotic. Aripiprazole commonly caused (being a perpetrator drug 94.6% of the time) potential DDIs with antidepressants (e.g., duloxetine, venlafaxine, mirtazapine), opioids (e.g., hydrocodone, oxycodone, tramadol) and metoprolol via the CYP2D6 isoform. Risperidone commonly caused (85.7%) potential DDIs with donepezil, lamotrigine and trazodone via the CYP3A4 isoform. Quetiapine exclusively suffered (100%) from potential DDIs with amlodipine, buspirone, omeprazole or topiramate via the CYP3A4 isoform. Antipsychotics are commonly prescribed to PACE participants for BPSD treatment and they may interact with other drugs used to treat comorbidities. A thorough review of concomitant medications will help mitigate the likelihood of potentially dangerous CYP-mediated DDIs involving antipsychotics.
Enhanced Dielectric Permittivity of Optimized Surface Modified of Barium Titanate Nanocomposites
Authors: Sundar Udhay , Lao Zichen , Cook-Chennault Kimberly
Published: April 2020

High permittivity polymer-ceramic nanocomposite dielectric films take advantage of the ease of flexibility in processing of polymers and the functionality of electroactive ceramic fillers. Hence, films like these may be applied to embedded energy storage devices for printed circuit electrical boards. However, the incompatibility of the hydrophilic ceramic filler and hydrophobic epoxy limit the filler concentration and therefore, dielectric permittivity of these materials. Traditionally, surfactants and core-shell processing of ceramic fillers are used to achieve electrostatic and steric stabilization for adequate ceramic particle distribution but, questions regarding these processes still remain. The purpose of this work is to understand the role of surfactant concentration ceramic particle surface morphology, and composite dielectric permittivity and conductivity. A comprehensive study of barium titanate-based epoxy nanocomposites was performed. Ethanol and 3-glycidyloxypropyltrimethoxysilan surface treatments were performed, where the best reduction in particle agglomeration, highest value of permittivity and the lowest value of loss were observed. The results demonstrate that optimization of coupling agent may lead to superior permittivity values and diminished losses that are ~2–3 times that of composites with non-optimized and traditional surfactant treatments.
Bio-Based Aromatic Copolyesters: Influence of Chemical Microstructures on Thermal and Crystalline Properties
Authors: Hu Keling
Published: April 2020

Aromatic copolyesters, derived from bio-based nipagin and eugenol, were synthesized with renewable 1,6-hexandiol as the spacer. Number-average, weight-average molecular weights (Mn, Mw), and polydispersity (D) values were determined by size exclusion chromatography (SEC). Chemical structures were confirmed by 1H NMR and 13C NMR spectroscopies. Chemical microstructure analysis suggested that the nipagin and eugenol-derived units were inserted into polymer chains in an arbitrary manner. Due to the short chain of 1,6-hexanediol, the splitting of magnetically different methylene carbons, adjacent to the alcohol-oxygens, proved to be more sensitive towards sequence distributions, at the dyed level, than those from 1,10-decanediol. Thermal gravimetric analysis (TGA) demonstrated that these polyester materials have excellent thermal stability (>360 °C), regardless of the content of eugenol-derived composition incorporated. Differential scanning calorimetric (DSC) and wide-angle X-ray diffraction (WXRD) experiments revealed the semicrystalline nature for this kind of copolyesters. The crystallinities gradually decreased with the increase of eugenol-derived composition. Thermal and crystalline properties were well discussed from the microscopic perspective. The point of this work lies in establishing guidance for future design and modification of high-performance polymer materials from the microscopic perspective.
Hot-melt Adhesive Bonding of Polyurethane/Fluorinated Polyurethane/Alkylsilane-Functionalized Graphene Nanofibrous Fabrics with Enhanced Waterproofness, Breathability, and Mechanical Properties
Authors: Liu Chunhui , Liao Xi , Shao Weili , Liu Fan , Ding Bin , Ren Gaihuan , Chu Yanyan , He Jianxin
Published: April 2020

Waterproof-breathable (WB) materials with outstanding waterproofness, breathability, and mechanical performance are critical in diverse consumer applications. Electrospun nanofibrous membranes with thin fiber diameters, small pore sizes, and high porosity have attracted significant attention in the WB fabric field. Hot-press treatment technology can induce the formation of inter-fiber fusion structures and hence improve the waterproofness and mechanical performance. By combining electrospinning and hot-press treatment technology, polyurethane/fluorinated polyurethane/thermoplastic polyurethane/alkylsilane-functionalized graphene (PU/FPU/TPU/FG) nanofiber WB fabric was fabricated. Subsequently, the morphologies, porous structure, hydrostatic pressure, water vapor transmission rate (WVTR), and stress–strain behavior of the nanofiber WB fabric were systematically investigated. The introduction of the hydrophobic FG sheet structure and the formation of the inter-fiber fusion structure greatly improved not only the waterproofness but also the mechanical performance of the nanofiber WB fabric. The optimized PU/FPU/TPU-50/FG-1.5 WB fabric exhibited an excellent comprehensive performance: a high hydrostatic pressure of 80.4 kPa, a modest WVTR of 7.6 kg m−2 d−1, and a robust tensile stress of 127.59 MPa, which could be used to achieve various applications. This work not only highlights the preparation of materials, but also provides a high-performance nanofiber WB fabric with huge potential application prospects in various fields.
In Vitro and In Vivo Comparison Study of Electrospun PLA and PLA/PVA/SA Fiber Membranes for Wound Healing
Authors: Bi Hongyan , Feng Tianyi , Li Binbin , Han Yingchao
Published: April 2020

Wound dressings can accelerate wound healing. The degradable polymer poly(lactic acid) (PLA) shows good mechanical properties and biocompatibility. Sodium alginate (SA) holds good biocompatibility, hemostasis, and high hygroscopicity. Poly(vinyl alcohol) (PVA) has good spinnability as a pharmaceutical excipient. Herein, we carried out a comparison study of electrospun PLA and PLA/PVA/SA fiber membranes for wound healing in vitro and in vivo. In this study, PLA and PLA/PVA/SA nanofiber membranes were fabricated through electrospinning to produce a highly porous and large specific surface area that could promote wound healing. In vitro experiments showed that PLA and PLA/PVA/SA nanofiber membranes could all provide good support for the growth of rat fibroblasts (L929). Moreover, rat fibroblasts displayed slightly better adhesion and proliferation on PLA/PVA/SA than on the PLA fiber membranes. The in vivo potentiality of the PLA and PLA/PVA/SA fiber membranes was assessed in rat models of skin defects in which the PLA and PLA/PVA/SA fiber membranes significantly improved wound healing compared to commercially available gauzes. No significant differences in wound healing were observed between PLA and PLA/PVA/SA fiber membranes in our study. Furthermore, Masson staining and PCR displayed the PLA fiber membrane promoted protein deposition compared to the PLA/PVA/SA fiber membrane. In addition, IHC suggested that PLA/PVA/SA dressing reduced the inflammatory response during early wound healing compared to the PLA fiber membrane. These findings highlight the utility of fiber membranes as novel wound-healing dressings.
Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review
Authors: Klimek Katarzyna , Ginalska Grazyna
Published: April 2020

Polymer scaffolds constitute a very interesting strategy for tissue engineering. Even though they are generally non-toxic, in some cases, they may not provide suitable support for cell adhesion, proliferation, and differentiation, which decelerates tissue regeneration. To improve biological properties, scaffolds are frequently enriched with bioactive molecules, inter alia extracellular matrix proteins, adhesive peptides, growth factors, hormones, and cytokines. Although there are many papers describing synthesis and properties of polymer scaffolds enriched with proteins or peptides, few reviews comprehensively summarize these bioactive molecules. Thus, this review presents the current knowledge about the most important proteins and peptides used for modification of polymer scaffolds for tissue engineering. This paper also describes the influence of addition of proteins and peptides on physicochemical, mechanical, and biological properties of polymer scaffolds. Moreover, this article sums up the major applications of some biodegradable natural and synthetic polymer scaffolds modified with proteins and peptides, which have been developed within the past five years.