bluuebunny/crossref_metadata_2025 huggingface dataset:
New Atypical Antipsychotics in the Treatment of Schizophrenia and Depression
Authors: Orzelska-Górka Jolanta , Mikulska Joanna , Wiszniewska Anna , Biała Grażyna
Published: September 2022

Schizophrenia and depression are heterogeneous disorders. The complex pathomechanism of the diseases imply that medication responses vary across patients. Many psychotropic drugs are available but achieving optimal therapeutic effect can be challenging. The evidence correlates well with clinical observations, suggesting that new atypical antipsychotic drugs are effective against negative and cognitive symptoms of schizophrenia, as well as against affective symptoms observed in depression. The purpose of this review presents the background and evidence for the use of the new second/third-generation antipsychotics (aripiprazole, cariprazine, lurasidone, asenapine, brexpiprazole, lumateperone, pimavanserin) in treatment of schizophrenia and depression. We have first provided a brief overview of the major neurobiological underpinnings of schizophrenia and depression. We then shortly discuss efficacy, safety and limitations of ongoing pharmacotherapy used in depression and schizophrenia. Mainly, we have focused this review on the therapeutic potential of new atypical antipsychotic drugs—currently existing—to be effective in psychotic, as well as in affective disorders.
Natural Radiosensitizers in Radiotherapy: Cancer Treatment by Combining Ionizing Radiation with Resveratrol
Authors: Komorowska Dominika , Radzik Tomasz , Kalenik Sebastian , Rodacka Aleksandra
Published: September 2022

Conventional cancer treatment is mainly based on the surgical removal of the tumor followed by radiotherapy and/or chemotherapy. When surgical removal is not possible, radiotherapy and, less often, chemotherapy is the only way to treat patients. However, despite significant progress in understanding the molecular mechanisms of carcinogenesis and developments in modern radiotherapy techniques, radiotherapy (alone or in combination) does not always guarantee treatment success. One of the main causes is the radioresistance of cancer cells. Increasing the radiosensitivity of cancer cells improves the processes leading to their elimination during radiotherapy and prolonging the survival of cancer patients. In order to enhance the effect of radiotherapy in the treatment of radioresistant neoplasms, radiosensitizers are used. In clinical practice, synthetic radiosensitizers are commonly applied, but scientists have recently focused on using natural products (phytocompounds) as adjuvants in radiotherapy. In this review article, we only discuss naturally occurring radiosensitizers currently in clinical trials (paclitaxel, curcumin, genistein, and papaverine) and those whose radiation sensitizing effects, such as resveratrol, have been repeatedly confirmed by many independent studies.
Immunization with Genetically Modified Trypanosomes Provides Protection against Transmissible Spongiform Encephalopathies
Authors: Triller Gianna , Garyfallos Dimitrios A. , Papavasiliou F. Nina , Sklaviadis Theodoros , Stavropoulos Pete , Xanthopoulos Konstantinos
Published: September 2022

Transmissible spongiform encephalopathies are incurable neurodegenerative diseases, associated with the conversion of the physiological prion protein to its disease-associated counterpart. Even though immunization against transmissible spongiform encephalopathies has shown great potential, immune tolerance effects impede the use of active immunization protocols for successful prophylaxis. In this study, we evaluate the use of trypanosomes as biological platforms for the presentation of a prion antigenic peptide to the host immune system. Using the engineered trypanosomes in an immunization protocol without the use of adjuvants led to the development of a humoral immune response against the prion protein in wild type mice, without the appearance of adverse reactions. The immune reaction elicited with this protocol displayed in vitro therapeutic potential and was further evaluated in a bioassay where immunized mice were partially protected in a representative murine model of prion diseases. Further studies are underway to better characterize the immune reaction and optimize the immunization protocol.
Short-Term Supplementation of Sodium Nitrate vs. Sodium Chloride Increases Homoarginine Synthesis in Young Men Independent of Exercise
Authors: Tsikas Dimitrios , Maassen Norbert , Thorns Antonie , Finkel Armin , Lützow Moritz , Röhrig Magdalena Aleksandra , Blau Larissa Sarah , Dimina Laurianne , Mariotti François , Beckmann Bibiana , Shushakov Vladimir , Jantz Mirja
Published: September 2022

The aim of the study was to investigate the effects of short-term oral administration of inorganic nitrate (NaNO3; n = 8) or placebo (NaCl; n = 9) (each 0.1 mmol/kg body weight/d for 9 days) on plasma amino acids, creatinine, and oxidative stress in healthy young men. At baseline, the plasma concentrations of amino acids did not differ between the groups. At the end of the study, the plasma concentrations of homoarginine (hArg; by 24%, p = 0.0001), citrulline and ornithine (Cit/Orn; by 16%, p = 0.015), and glutamine/glutamate (Gln/Glu; by 6%, p = 0.0003) were higher in the NaNO3 group compared to the NaCl group. The plasma concentrations of sarcosine (Sarc; by 28%, p < 0.0001), tyrosine (by 14%, p = 0.0051), phenylalanine (by 8%, p = 0.0026), and tryptophan (by 8%, p = 0.0047) were lower in the NaNO3 group compared to the NaCl group. These results suggest that nitrate administration affects amino-acid metabolism. The arginine/glycine amidinotransferase (AGAT) catalyzes two reactions: (1) the formation of l-homoarginine (hArg) and l-ornithine (Orn) from l-arginine (Arg) and l-lysine (Lys): Arg + Lys <−> hArg + Orn, with equilibrium constant Kharg; (2) the formation of guanidinoacetate (GAA) and Orn from Arg and glycine (Gly): Arg + Gly <−> GAA + Orn, with equilibrium constant Kgaa. The plasma Kgaa/KhArg ratio was lower in the NaNO3 group compared to the NaCl group (1.57 vs. 2.02, p = 0.0034). Our study suggests that supplementation of inorganic nitrate increases the AGAT-catalyzed synthesis of hArg and decreases the N-methyltransferase-catalyzed synthesis of GAA, the precursor of creatine. To our knowledge, this is the first study to demonstrate elevation of hArg synthesis by inorganic nitrate supplementation. Remarkably, an increase of 24% corresponds to the synthesis capacity of one kidney in healthy humans. Differences in the association between plasma concentrations of amino acids in the NaNO3 and NaCl groups suggest changes in amino-acid homeostasis. Plasma concentrations of the oxidative stress marker malondialdehyde (MDA) did not change after supplementation of NaNO3 or NaCl over the whole exercise time range. Plasma nitrite concentration turned out to be a more discriminant marker of NaNO3 ingestion than plasma nitrate (area under the receiver operating characteristic curve: 0.951 vs. 0.866, p < 0.0001 each).
Machine Learning-Based Virtual Screening for the Identification of Cdk5 Inhibitors
Authors: Di Stefano Miriana , Galati Salvatore , Ortore Gabriella , Caligiuri Isabella , Rizzolio Flavio , Ceni Costanza , Bertini Simone , Bononi Giulia , Granchi Carlotta , Macchia Marco , Poli Giulio , Tuccinardi Tiziano
Published: September 2022

Cyclin-dependent kinase 5 (Cdk5) is an atypical proline-directed serine/threonine protein kinase well-characterized for its role in the central nervous system rather than in the cell cycle. Indeed, its dysregulation has been strongly implicated in the progression of synaptic dysfunction and neurodegenerative diseases, such as Alzheimer’s disease (AD) and Parkinson’s disease (PD), and also in the development and progression of a variety of cancers. For this reason, Cdk5 is considered as a promising target for drug design, and the discovery of novel small-molecule Cdk5 inhibitors is of great interest in the medicinal chemistry field. In this context, we employed a machine learning-based virtual screening protocol with subsequent molecular docking, molecular dynamics simulations and binding free energy evaluations. Our virtual screening studies resulted in the identification of two novel Cdk5 inhibitors, highlighting an experimental hit rate of 50% and thus validating the reliability of the in silico workflow. Both identified ligands, compounds CPD1 and CPD4, showed a promising enzyme inhibitory activity and CPD1 also demonstrated a remarkable antiproliferative activity in ovarian and colon cancer cells. These ligands represent a valuable starting point for structure-based hit-optimization studies aimed at identifying new potent Cdk5 inhibitors.
Oxidation of p-[125I]Iodobenzoic Acid and p-[211At]Astatobenzoic Acid Derivatives and Evaluation In Vivo
Authors: Li Yawen , Chyan Ming-Kuan , Hamlin Donald K. , Nguyen Holly , Corey Eva , Wilbur D. Scott
Published: September 2022

The alpha particle-emitting radionuclide astatine-211 (211At) is of interest for targeted radiotherapy; however, low in vivo stability of many 211At-labeled cancer-targeting molecules has limited its potential. As an alternative labeling method, we evaluated whether a specific type of astatinated aryl compound that has the At atom in a higher oxidation state might be stable to in vivo deastatination. In the research effort, para-iodobenzoic acid methyl ester and dPEG4-amino acid methyl ester derivatives were prepared as HPLC standards. The corresponding para-stannylbenzoic acid derivatives were also prepared and labeled with 125I and 211At. Oxidization of the [125I]iodo- and [211At]astato-benzamidyl-dPEG4-acid methyl ester derivatives provided materials for in vivo evaluation. A biodistribution was conducted in mice with coinjected oxidized 125I- and 211At-labeled compounds. The oxidized radioiodinated derivative was stable to in vivo deiodination, but unfortunately the oxidized [211At]astatinated benzamide derivative was found to be unstable under the conditions of isolation by radio-HPLC (post animal injection). Another biodistribution study in mice evaluated the tissue concentrations of coinjected [211At]NaAtO3 and [125I]NaIO3. Comparison of the tissue concentrations of the isolated material from the oxidized [211At]benzamide derivative with those of [211At]astatate indicated the species obtained after isolation was likely [211At]astatate.
OsABT Is Involved in Abscisic Acid Signaling Pathway and Salt Tolerance of Roots at the Rice Seedling Stage
Authors: Wen Danni , Bao Lingran , Huang Xuanzhu , Qian Xueduo , Chen Eryong , Shen Bo
Published: September 2022

Rice is a staple cereal crop worldwide, and increasing its yields is vital to ensuring global food security. Salinity is a major factor that affects rice yield. Therefore, it is necessary to investigate salt tolerance mechanisms in rice. Proteins containing WD40 repeats play important roles in eukaryotic development and environmental adaptation. Here, we showed that overexpression of OsABT, a gene encoding a WD40-repeat protein, enhanced salt tolerance in rice seedlings by regulating root activity, relative conductivity, malondialdehyde and H2O2 content, and O2•− production rate. Root ion concentrations indicated that OsABT overexpression lines could maintain lower Na+ and higher K+/Na+ ratios and upregulated expression of salt-related genes OsSOS1 and OsHAK5 compared with the wild-type (WT) Nipponbare plants. Furthermore, Overexpression of OsABT decreased the abscisic acid (ABA) content, while downregulating the ABA synthesis genes OsNCED3 and OsNCED4 and upregulating the ABA catabolic gene OsABA8ox2. The yeast two-hybrid and bimolecular fluorescence complementation analyses showed that OsABT interacted with the ABA receptor proteins OsPYL4, OsPYL10, and PP2C phosphatase OsABIL2. A transcriptome analysis revealed that the differentially expressed genes between OsABT overexpression lines and WT plants were enriched in plant hormone signal transduction, including ABA signaling pathway under salt stress. Thus, OsABT can improve the salt tolerance in rice seedling roots by inhibiting reactive oxygen species accumulation, thereby regulating the intracellular Na+/K+ balance, ABA content, and ABA signaling pathway.
Radioactive Waste Immobilization Using Vitreous Materials for Facilities in a Safe and Resilient Infrastructure Classified by Multivariate Exploratory Analyses
Authors: Nascimento Marcio Luis Ferreira , Cassar Daniel Roberto , Ciolini Riccardo , Souza Susana Oliveira , d’Errico Francesco
Published: September 2022

A database of 479 glass formulations used to immobilize radioactive wastes for facilities in a safe and resilient infrastructure was analyzed, searching for underlying statistical patterns and associated glass performance features. The analyzed data cover many oxides, including SiO2, B2O3, Na2O, Fe2O3, and some fluorides. Borosilicates were the most common glasses (60.1%), while silicates were only 11.9%. In addition to these two families, five radioactive waste vitrification matrices were identified: Boroaluminosilicates, iron phosphates, aluminosilicates, sodium iron phosphates, and boroaluminates, totaling seven glass families. Almost all compositions (97.7%) contained sodium oxide, followed by silica (91.4%), iron (82.7%), boron (73.7%), phosphorus (54.9%), and cesium oxides (26.1%). Multivariate exploratory methods were applied to analyze and classify glass compositions using hierarchical and non-hierarchical (K-means) clusters and principal component analysis. Four main clusters were observed, the largest comprising 417 formulations containing mainly silicates, borosilicates, aluminosilicates, and boroaluminosilicates; two principal components, representing 73.75% of all compositions, emerge from these four clusters derived from a covariance analysis. The principal components and four clusters may be associated with the following glass features in terms of glass compositions: liquidus temperature, glass transition temperature, density, resistivity, microhardness, and viscosity. Some general underlying properties emerged from our classification and are discussed.
Nutritional Composition of Black Soldier Fly Larvae (Hermetia illucens L.) and Its Potential Uses as Alternative Protein Sources in Animal Diets: A Review
Authors: Lu Shengyong , Taethaisong Nittaya , Meethip Weerada , Surakhunthod Jariya , Sinpru Boontum , Sroichak Thakun , Archa Pawinee , Thongpea Sorasak , Paengkoum Siwaporn , Purba Rayudika Aprilia Patindra , Paengkoum Pramote
Published: September 2022

The rapidly growing population has increased demand for protein quantities and, following a shortage of plant-based feed protein sources and the prohibition of animal-based feed protein, has forced the search for new sources of protein. Therefore, humans have turned their attention to edible insects. Black soldier fly larvae (BSFL) (Hermetia illucens L.) are rich in nutrients such as fat, protein and high-quality amino acids and minerals, making them a good source of protein. Furthermore, BSFL are easily reared and propagated on any nutrient substrate such as plant residues, animal manure and waste, food scraps, agricultural byproducts, or straw. Although BSFL cannot completely replace soybean meal in poultry diets, supplementation of less than 20% has no negative impact on chicken growth performance, biochemical indicators and meat quality. In pig studies, although BSFL supplementation did not have any negative effect on growth performance and meat quality, the feed conversion ratio (FCR) was reduced. There is obviously less research on the feeding of BSFL in pigs than in poultry, particularly in relation to weaning piglets and fattening pigs; further research is needed on the supplementation level of sows. Moreover, it has not been found that BSFL are used in ruminants, and the next phase of research could therefore study them. The use of BSFL in animal feed presents some challenges in terms of cost, availability and legal and consumer acceptance. However, this should be considered in the context of the current shortage of protein feed and the nutritional value of BSFL, which has important research significance in animal production.
RNA Interference of Chitin Synthase 2 Gene in Liriomyza trifolii through Immersion in Double-Stranded RNA
Authors: Chang Ya-Wen , Wang Yu-Cheng , Yan Yu-Qing , Xie Hong-Fang , Yuan Deng-Rong , Du Yu-Zhou
Published: September 2022

Liriomyza trifolii is an important invasive pest that infects horticultural vegetables, displaying a strong competitive advantage and showing great potential for inflicting harm. Chitin synthase is one of the key enzymes in insect chitin metabolism and plays an important role in insect growth and development. In this study, a chitin synthase (CHS) transcript of L. trifolii was cloned, and the results showed that LtCHS belongs to the CHS2 family. The expression analysis indicated the presence of the highest abundance of LtCHS2 in the pupae at different developmental stages but showed no significant difference among different tissues in the adult. Furthermore, a dsRNA immersion method was developed for RNA interference (RNAi) in L. trifolii using LtCHS2 transcript. RNAi can significantly reduce the expression of LtCHS2 in pupae, and the emergence rate of the pupae was significantly lower than that of the control. The results provide a theoretical basis for exploring the role of chitin synthase gene in L. trifolii and proposing new pest control strategies.
Bta06987, Encoding a Peptide of the AKH/RPCH Family: A Role of Energy Mobilization in Bemisia tabaci
Authors: Fan Xiaofan , Liu Yong , Zhang Zhuo , Zhang Zhanhong , Peng Jing , Gao Yang , Zheng Limin , Chen Jianbin , Du Jiao , Yan Shuo , Zhou Xuguo , Shi Xiaobin , Zhang Deyong
Published: September 2022

A neuropeptide precursor encoded by Bta06987 associates with AKH neuropeptide. In the AKH/RPCH family, these members have been demonstrated to participate in energy mobilization in many insects. In our research, the Bta06987 gene from Bemisia tabaci was cloned, and the amino acid sequence analysis was performed. During the starvation of B. tabaci, the mRNA level of Bta06987 showed a significant elevation. We investigated the functions of Bta06987 in B. tabaci using RNA interference (RNAi), and the adult females of B. tabaci after being fed with dsBta06987 showed a higher glycogen and triglyceride levels and lower trehalose content than the control. Furthermore, in the electrical penetration graph (EPG) experiment, B. tabaci showed changes in feeding behavior after feeding with dsBta06987, such as the reduction in parameters of E waveform percentage and total feeding time. Our findings might be helpful in developing strategies to control pest and plant virus transmission.
In Vitro Study of Ineffective Erythropoiesis in Thalassemia: Diverse Intrinsic Pathophysiological Features of Erythroid Cells Derived from Various Thalassemia Syndromes
Authors: Kaewsakulthong Woratree , Suriyun Thunwarat , Chumchuen Sukanya , Anurathapan Usanarat , Hongeng Suradej , Fucharoen Suthat , Sripichai Orapan
Published: September 2022

Defective hemoglobin production and ineffective erythropoiesis contribute to the pathophysiology of thalassemia syndromes. Previous studies in the field of erythropoiesis mainly focused on the severe forms of thalassemia, such as β-thalassemia major, while mechanisms underlying the pathogenesis of other thalassemia syndromes remain largely unexplored. The current study aimed to investigate the intrinsic pathophysiological properties of erythroid cells derived from the most common forms of thalassemia diseases, including α-thalassemia (hemoglobin H and hemoglobin H-Constant Spring diseases) and β-thalassemia (homozygous β0-thalassemia and β0-thalassemia/hemoglobin E diseases), under an identical in vitro erythroid culture system. Cell proliferation capacity, differentiation velocity, cell death, as well as globin synthesis and the expression levels of erythropoiesis modifying factors were determined. Accelerated expansion was found in erythroblast cells derived from all types of thalassemia, with the highest degree in β0-thalassemia/hemoglobin E. Likewise, all types of thalassemia showed limited erythroid cell differentiation, but each of them manifested varying degrees of erythroid maturation arrest corresponding with the clinical severity. Robust induction of HSP70 transcripts, an erythroid maturation-related factor, was found in both α- and β-thalassemia erythroid cells. Increased cell death was distinctly present only in homozygous β0-thalassemia erythroblasts and associated with the up-regulation of pro-apoptotic (Caspase 9, BAD, and MTCH1) genes and down-regulation of the anti-apoptotic BCL-XL gene.
Relationship between Leptin and Insulin Resistance among Community—Dwelling Middle-Aged and Elderly Populations in Taiwan
Authors: Shih Yu-Lin , Huang Tzu-Cheng , Shih Chin-Chuan , Chen Jau-Yuan
Published: September 2022

The relationship between leptin and insulin resistance among middle-aged and elderly populations in Asia is seldom reported. Our research included 398 middle-aged and elderly Taiwanese individuals. First, we divided participants into three groups according to the tertiles of the homeostasis model assessment of insulin resistance (HOMA-IR) to analyze the parameters between each group. Pearson’s correlation was then applied to calculate the correlation between HOMA-IR and cardiometabolic risk factors after adjusting for age. A scatter plot indicated a relationship between serum leptin levels and the HOMA-IR index. Finally, the coefficients of the serum leptin level and HOMA-IR were assessed by multivariate linear regression. The participants in the high HOMA-IR index group were more likely to have higher serum leptin levels. Meanwhile, the HOMA-IR index was positively correlated with serum leptin levels, even after adjusting for age. Serum leptin levels were positively correlated with the HOMA-IR index (β = 0.226, p < 0.01) in the multivariate linear regression after adjusting for age, sex, smoking, drinking, BMI, triglycerides, systolic blood pressure, fasting plasma glucose, uric acid, ALT, and creatinine. Furthermore, the leptin–creatinine ratio also showed a significantly positive relationship with HOMA-IR in the same multivariate linear regression model. In conclusion, serum leptin levels showed a positive relationship with insulin resistance in middle-aged and elderly people in Taiwan. Furthermore, serum leptin levels may be an independent risk factor for insulin resistance according to our study.
A Focus on the Role of DSC-PWI Dynamic Radiomics Features in Diagnosis and Outcome Prediction of Ischemic Stroke
Authors: Guo Yingwei , Yang Yingjian , Cao Fengqiu , Wang Mingming , Luo Yu , Guo Jia , Liu Yang , Zeng Xueqiang , Miu Xiaoqiang , Zaman Asim , Lu Jiaxi , Kang Yan
Published: September 2022

Background: The ability to accurately detect ischemic stroke and predict its neurological recovery is of great clinical value. This study intended to evaluate the performance of whole-brain dynamic radiomics features (DRF) for ischemic stroke detection, neurological impairment assessment, and outcome prediction. Methods: The supervised feature selection (Lasso) and unsupervised feature-selection methods (five-feature dimension-reduction algorithms) were used to generate four experimental groups with DRF in different combinations. Ten machine learning models were used to evaluate their performance by ten-fold cross-validation. Results: In experimental group_A, the best AUCs (0.873 for stroke detection, 0.795 for NIHSS assessment, and 0.818 for outcome prediction) were obtained by outstanding DRF selected by Lasso, and the performance of significant DRF was better than the five-feature dimension-reduction algorithms. The selected outstanding dimension-reduction DRF in experimental group_C obtained a better AUC than dimension-reduction DRF in experimental group_A but were inferior to the outstanding DRF in experimental group_A. When combining the outstanding DRF with each dimension-reduction DRF (experimental group_B), the performance can be improved in ischemic stroke detection (best AUC = 0.899) and NIHSS assessment (best AUC = 0.835) but failed in outcome prediction (best AUC = 0.806). The performance can be further improved when combining outstanding DRF with outstanding dimension-reduction DRF (experimental group_D), achieving the highest AUC scores in all three evaluation items (0.925 for stroke detection, 0.853 for NIHSS assessment, and 0.828 for outcome prediction). By the method in this study, comparing the best AUC of Ft-test in experimental group_A and the best_AUC in experimental group_D, the AUC in stroke detection increased by 19.4% (from 0.731 to 0.925), the AUC in NIHSS assessment increased by 20.1% (from 0.652 to 0.853), and the AUC in prognosis prediction increased by 14.9% (from 0.679 to 0.828). This study provided a potential clinical tool for detailed clinical diagnosis and outcome prediction before treatment.
Right Heart Catheterization in Pediatric Pulmonary Arterial Hypertension: Insights and Outcome from a Large Tertiary Center
Authors: Xu Zhuoyuan , Zhang Hongsheng , Arvanitaki Alexandra , Zhang Chen , Li Qiangqiang , Keller Bradley B. , Gu Hong
Published: September 2022

Aim: To define the clinical characteristics, hemodynamics, and adverse events for pediatric patients with pulmonary arterial hypertension (PAH) undergoing right heart catheterization (RHC). Methods: The large referral single center data of 591 diagnostic RHC procedures performed between 2005 and 2020 on pediatric PAH patients was retrospectively collected and analyzed. Results: A total of 591 RHC procedures performed on 469 patients with congenital heart disease (CHD)-PAH (median age 8.8 years, 7.9% New York Heart Association (NYHA) class > II, 1.5% with syncope) and 122 patients with idiopathic PAH (median age of 9.0 years, 27.0% NYHA class > II, 27.0% with syncope) were included. Of those, 373 (63.1%) procedures were performed under general anesthesia. Eighteen patients (18/591, 3.0%) suffered adverse events (mainly pulmonary hypertensive crisis, PHC, n = 17) during the RHC procedure, including 14 idiopathic pulmonary arterial hypertension (IPAH) patients and 4 CHD-PAH patients, and one IPAH patient died in hospital 63 hours after RHC. The risk of developing PHC was significantly increased in patients with IPAH (OR = 14.02, 95%CI: 4.49–43.85, p < 0.001), atrial blood gas pH < 7.35 (OR = 12.504, 95%CI: 3.545–44.102, p < 0.001) and RAP > 14 mmHg (OR = 10.636, 95%CI: 3.668–30.847, p < 0.001). Conclusions: RHC is generally a low-risk procedure in pediatric patients with PAH. However, PHC occur in approximately 3% of patients. Therefore, RHC should be performed in a large, experienced referral pediatric cardiology center, especially in pediatric patients with IPAH requiring general anesthesia.
Bleeding and Thrombotic Issues during Extracorporeal Membrane Oxygenation
Authors: Ranucci Marco , Cotza Mauro , Di Dedda Umberto
Published: September 2022

Extracorporeal Membrane Oxygenation (ECMO) is an advanced life support modality for patients with respiratory or cardiac failure refractory to standard therapy [...]
New Devitalized Freeze-Dried Human Umbilical Cord Amniotic Membrane as an Innovative Treatment of Ocular Surface Defects: Preclinical Results
Authors: Cognard Sophie , Barnouin Laurence , Bosc Justine , Gindraux Florelle , Robin Marie-Claire , Douet Jean-Yves , Thuret Gilles
Published: September 2022

A preclinical study was performed to investigate the efficacy and safety of a new viral inactivated, devitalized, freeze-dried and gamma-sterilized human umbilical cord amniotic membrane (lhUC-AM) for the treatment of deep scleral and corneal defects with or without perforation. Firstly, lhUC-AM was investigated on experimental deep sclerectomy in rabbit eyes (n = 12) and compared to autograft (n = 4) on cross section histology. Secondly, lhUC-AM was studied on a selected series of uncontrolled cases of corneal defects (n = 18) with or without perforation, in dogs and cats. lhUC-AM tolerance, reconstruction of the deep corneal lesion and recovery of the structural aspect of the tissue were followed post-surgery. In experimental deep sclerectomy, histology showed that the lhUC-AM was well tolerated and degraded completely in 45 days while allowing an overall quality and kinetic of scleral regeneration, similar to autograft. In the clinical situations, lhUC-AM was well tolerated, with ocular inflammatory signs quickly decreasing after surgery. Mean follow-up was 16.40 ± 11.43 months. In 15 out of 18 cases, lhUC-AM allowed ocular surface wound healing. The ocular surface was fully reconstructed three months after surgery. This study suggests a good safety and efficacy profile of lhUC-AM in the treatment of deep corneal or scleral defect in animals. This new tissue should now facilitate the treatment of severe ocular surface diseases in humans.
Compressive Properties and Degradable Behavior of Biodegradable Porous Zinc Fabricated with the Protein Foaming Method
Authors: Ge Qiqi , Liu Xiaoqian , Qiao Aike , Mu Yongliang
Published: September 2022

A new protein foaming–consolidation method for preparing porous zinc was developed using three proteins (egg white protein (EWP), bovine bone collagen protein (BBCP), and fish bone collagen protein (FBCP)) as both consolidating and foaming agents. The preparation route utilized powder mixing and sintering processing, which could be divided into three steps: slurry preparation, low-temperature foaming, and high-temperature sintering. The morphological characteristics of the pore structures revealed that the porous zinc had an interconnected open-cell structure. Compared to the porous zinc prepared with EWP or BBCP, the porous zinc prepared with FBCP possessed the largest average pore size and the highest compressive properties. The porosity of the porous zinc increased with the stirring time, the content of protein and sucrose, and higher sintering temperatures. Moreover, a compression test and immersion test were performed to investigate the stress–strain behavior and corrosion properties of the resulting porous zinc. A fluctuated stress plateau could be found due to the brittle fracture of the porous cells. The porous zinc prepared with FBCP showed the highest compressive strength and elastic modulus. The corrosion rate of the porous zinc obtained through an immersion test in vitro using simulated bodily fluids on the thirty-second day was close to 0.02 mm/year. The corresponding corrosion mechanism of porous zinc was also discussed.
Estimation of Person Ability under Rapid and Effortful Responding
Authors: Sideridis Georgios , Alahmadi Maisa
Published: September 2022

The goal of the present study was to extend earlier work on the estimation of person theta using maximum likelihood estimation in R by accounting for rapid guessing. This paper provides a modified R function that accommodates person thetas using the Rasch or 2PL models and implements corrections for the presence of rapid guessing or informed guessing behaviors. Initially, a sample of 200 participants was generated using Mplus in order to demonstrate the use of the function with the full sample and a single participant in particular. Subsequently, the function was applied to data from the General Aptitude Test (GAT) and the measurement of cognitive ability. Using a sample of 8500 participants, the present R function was demonstrated. An illustrative example of a single participant, assumed to be either a rapid responder or a successful guesser, is provided using MLE and BME. It was concluded that the present function can contribute to a more valid estimation of person ability.
High-Frequency Responses of the Blue Mussel (Mytilus edulis) Feeding and Ingestion Rates to Natural Diets
Authors: Steeves Laura , Agüera Antonio , Filgueira Ramón , Strand Øivind , Strohmeier Tore
Published: September 2022

The feeding activity of bivalves is understood to change in response to a suite of environmental conditions, including food quantity and quality. It has been hypothesized that, by varying feeding rates in response to the available diet, bivalves may be able to maintain relatively stable ingestion rates, allowing them to have constant energy uptake despite changes in food availability. The purpose of this study was to determine if the blue mussel Mytilus edulis responds to fluctuations in natural diets by changing feeding rates to maintain constant ingestion rates. Three four-day experiments were conducted to measure pumping and ingestion rates in response to natural fluctuations in food concentration (chlorophyll a). Experiments were conducted in a flow-through system over the spring season in south-western Norway. Pumping and ingestion rates were measured with high temporal resolution (every 20 min), which permitted the observation of the intra- and interindividual variability of feeding rates. Results show pumping rates varying within individuals over 4 days, and some individuals pumping on average at high rates (~5 Lh−1), and some at low (~1 Lh−1), despite being held in similar conditions. The pumping rate was generally not related to changes in food availability, and population-level ingestion rates increased with increasing food availability. These results suggest that, for this population of M. edulis, feeding rates may not vary with the available diet to produce constant ingestion over time.
Biological and Proteomic Characterization of the Anti-Cancer Potency of Aqueous Extracts from Cell-Free Coelomic Fluid of Arbacia lixula Sea Urchin in an In Vitro Model of Human Hepatocellular Carcinoma
Authors: Luparello Claudio , Branni Rossella , Abruscato Giulia , Lazzara Valentina , Sugár Simon , Arizza Vincenzo , Mauro Manuela , Di Stefano Vita , Vazzana Mirella
Published: September 2022

Echinoderms are an acknowledged source of bioactive compounds exerting various beneficial effects on human health. Here, we examined the potential in vitro anti-hepatocarcinoma effects of aqueous extracts of the cell-free coelomic fluid obtained from the sea urchin Arbacia lixula using the HepG2 cell line as a model system. This was accomplished by employing a combination of colorimetric, microscopic and flow cytometric assays to determine cell viability, cell cycle distribution, the possible onset of apoptosis, the accumulation rate of acidic vesicular organelles, mitochondrial polarization, cell redox state and cell locomotory ability. The obtained data show that exposed HepG2 cells underwent inhibition of cell viability with impairment of cell cycle progress coupled to the onset of apoptotic death, the induction of mitochondrial depolarization, the inhibition of reactive oxygen species production and acidic vesicular organelle accumulation, and the block of cell motile attitude. We also performed a proteomic analysis of the coelomic fluid extract identifying a number of proteins that are plausibly responsible for anti-cancer effects. Therefore, the anti-hepatocarcinoma potentiality of A. lixula’s preparation can be taken into consideration for further studies aimed at the characterization of the molecular mechanism of cytotoxicity and the development of novel prevention and/or treatment agents.
On the Predation of Doliolids (Tunicata, Thaliacea) on calanoid Copepods
Authors: Köster Marion , Paffenhöfer Gustav-Adolf
Published: September 2022

The main goal of this contribution was to determine the effect of predation of the often abundant to dominant doliolid Dolioletta gegenbauri (Tunicata, Thaliacea) on the abundance of co-occurring planktonic copepods by feeding on their eggs. Previous oceanographic investigations revealed that doliolids had ingested eggs of small calanoid copepods. The ecological significance of such feeding could not be quantified completely because the environmental abundance of such eggs was not known. In this study, the eggs and nauplii of the neritic calanoid Paracalanus quasimodo (Crustacea, Copepoda) were offered to gonozooids and phorozooids of D. gegenbauri with a 6–6.5 mm length together with three species of phytoplankton; i.e., simulating diet conditions on the shelf. We hypothesized that copepod eggs of a similar size as food particles would be readily ingested whereas small nauplii, which could escape, would hardly be eaten by the doliolids. Our results revealed that doliolids have the potential to control small calanoids by ingesting their eggs at high rates but not their nauplii or later stages. Late copepodid stages and adults of co-occurring calanoid species could cause less mortality because they prey less on such eggs than doliolids of a similar weight. However, certain abundant omnivorous calanoid species with pronounced perception and/or capture abilities can prey successfully on the nauplii of small calanoids.
Identification of Candidate Ice Nucleation Activity (INA) Genes in Fusarium avenaceum by Combining Phenotypic Characterization with Comparative Genomics and Transcriptomics
Authors: Yang Shu , Rojas Mariah , Coleman Jeffrey J. , Vinatzer Boris A.
Published: September 2022

Ice nucleation activity (INA) is the capacity of certain particles to catalyze ice formation at temperatures higher than the temperature at which pure water freezes. INA impacts the ratio of liquid to frozen cloud droplets and, therefore, the formation of precipitation and Earth’s radiative balance. Some Fusarium strains secrete ice-nucleating particles (INPs); they travel through the atmosphere and may thus contribute to these atmospheric processes. Fusarium INPs were previously found to consist of proteinaceous aggregates. Here, we determined that in F. avenaceum, the proteins forming these aggregates are smaller than 5 nm and INA is higher after growth at low temperatures and varies among strains. Leveraging these findings, we used comparative genomics and transcriptomics to identify candidate INA genes. Ten candidate INA genes that were predicted to encode secreted proteins were present only in the strains that produced the highest number of INPs. In total, 203 candidate INA genes coding for secreted proteins were induced at low temperatures. Among them, two genes predicted to encode hydrophobins stood out because hydrophobins are small, secreted proteins that form aggregates with amphipathic properties. We discuss the potential of the candidate genes to encode INA proteins and the next steps necessary to identify the molecular basis of INA in F. avenaceum.
A Green Infrastructure Planning Approach: Improving Territorial Cohesion through Urban-Rural Landscape in Vojvodina, Serbia
Authors: Bajić Luka , Vasiljević Nevena , Čavlović Dragana , Radić Boris , Gavrilović Suzana
Published: September 2022

Spatial and urban planning are directed towards achieving territorial cohesion as one of the sustainable development goals. Considering the hybrid concept of green infrastructure, this paper aims to provide an “ecological model” of achieving territorial cohesion in spatial and urban planning. Based on the connectivity level analysis between green infrastructure elements (green infrastructure hubs, nodes, gateways and dots), application of the GI concept guides the development of spatial planning scenarios. The application of Voronoi diagrams and landscape graph-based principles contribute to defining the shortest distances between green infrastructure elements, which resulted in the definition of the significance of structural and functional arrangement of green infrastructure dots in the matrix of the urban rural continuum in the territory of the urban-rural landscape of three case studies in Vojvodina, Serbia (Novi Sad, Subotica, Zrenjanin). As a result of this study, green infrastructure dots showed a great potential of application at the local level by developing them through landscape design with creative and artistic elements in order to achieve higher level of cohesion through visual attractivity, multifunctionality and recreation. The level of connectivity between elements of green infrastructure should be considered as an indicator of the sustainable spatial development goals achievement, in the field of nature conservation and territorial and social cohesion.
Mapping Ecosystem Service Supply–Demand Bundles for an Integrated Analysis of Tradeoffs in an Urban Agglomeration of China
Authors: Zhong Zhen , Fang Xuening , Zhang Yu , Shu Xianfang , Guo Dan
Published: September 2022

Ecosystem service (ES) bundles are widely used approaches to analyze ES tradeoffs in urban agglomeration. However, few ES bundle studies considered both the supply and demand sided. The objective of this study was to map ES supply–demand bundles to comprehensively analyze the ES tradeoffs in the Yangtze River Delta using cluster analysis, correlation analysis, hotspot analysis, and principal component analysis. We found that: (1) Both the supply and demand of ES are unevenly distributed with highly spatial autocorrelation. (2) There are significant tradeoffs and synergies between ES in terms of supply–supply, demand–demand, and supply–demand. (3) Four ES supply–demand bundle types were identified with distinct ES supply and demand relationships. (4) Land-use types, urban intensity, and climatic factors are the main social-ecological factors that distinguish the four identified clusters. The identified ES supply–demand clusters can support the main functional zoning of the Yangtze River Delta. Our results also suggest that land sharing is a more appropriate approach for the sustainable development of the Yangtze River Delta considering the balance of multiple ES supply and ES demand.
Phototransistors Based on hBN-Encapsulated NiPS3
Authors: Liu Yingjia , Sun Xingdan
Published: September 2022

Transition metal phosphorous trichalcogenides (MPX3) have been extensively investigated as photodetectors due to their wide-bandgap semiconductor properties. However, the research involved in the photoresponses at low temperatures remain blank. Here, hexagonal boron nitride (hBN)-encapsulated NiPS3 field effect transistors were fabricated by using the dry-transfer technique, indicating strong stability under atmospheric environments. The NiPS3 devices with the thickness of 10.4 nm, showed broad photoresponses from near-infrared to ultraviolet radiation at the liquid nitrogen temperature, and the minimum of rise time can reach 30 ms under the wavelength of 405 nm. The mechanism of temperature-dependent photoresponses can be deduced by competition between Schottky barrier height and thermal fluctuation. Our findings provide insights into superior phototransistors in few-layered NiPS3 for ultrasensitive light detection.
Fluctuation Analysis of a Soft-Extreme Shock Reliability Model
Authors: Dshalalow Jewgeni H. , White Ryan T.
Published: September 2022

In this paper, we deal with a mixed reliability system decaying from natural wear, occasional soft and hard shocks that eventually lead the system to failure. The aging process alone is linear and it is escalated through soft shocks such that they lead to the system’s soft failure when the combined damage exceeds a threshold M. The other threat is that posed by occasional hard shocks. When the total number of them identified as critical (each critical shock exceeds a fixed threshold H) reaches N, the system becomes disabled. With N=1, a critical shock is extreme. The arrival stream of shocks is a renewal process marked by soft and hard shocks. We establish a formula for a closed form functional containing system’s time-to-failure, the state of the system upon its failure, and other useful statistical characteristics of the system using and embellishing fluctuation analysis and operational calculus. Special cases provide tame expressions that are computed and validated by simulation.
A Novel Driving-Strategy Generating Method of Collision Avoidance for Unmanned Ships Based on Extensive-Form Game Model with Fuzzy Credibility Numbers
Authors: Cui Haotian , Zhang Fangwei , Li Mingjie , Cui Yang , Wang Rui
Published: September 2022

This study aims to solve the problem of intelligent collision avoidance of unmanned ships at sea, and it proposes a novel driving strategy generating method of collision avoidance based on an extensive-form game mode with fuzzy credibility numbers. The innovation of this study is to propose an extensive-form game model of unmanned ships under the situation of two-sides clamping and verify the validity by fuzzy credibility. Firstly, this study divides the head-on situation of the ship at sea quantitatively to help the unmanned ship take targeted measures when making collision avoidance decisions. Secondly, this study adopts an extensive-form game model to model the problem of collision avoidance of an unmanned ship in the case of clamping on two sides. Thirdly, the extensive-form game model is organically combined with the fuzzy credibility degree to judge whether the collision avoidance game of unmanned ship achieves the optimal collision avoidance result. The effectiveness of the introduced game model is verified by case analysis and simulation. Finally, an illustrative example shows that the proposed mathematical model can better help unmanned ships make real-time game decisions at sea in the scenario of two-sides clamping effectively.
A D2D Group Communication Scheme Using Bidirectional and InCremental A-Star Search to Configure Paths
Authors: Lai Wei Kuang , Shieh Chin-Shiuh , Yang Chao-Ping
Published: September 2022

The Device-to-Device (D2D) communication introduced in 3GPP’s Release 12 is a promising option for the accommodation of increasing traffic demand and the alleviation of core networks’ loading. The D2D communication mode prevails in scenarios where mobile users in proximity form a communication group sharing the same interest in digital content. A user can download intended content from peers in the same communication group rather than from the Internet via the base station. This article addresses the routing issue within D2D communication groups. It is, in effect, a path selection problem. We define a utility function considering both delay and throughput. The path selection problem can be formulated as an NP-hard optimization problem. A Bidirectional and InCremental A-star (BICA*) algorithm incorporating the concept of bidirectional search and lifelong planning is developed to tackle the NP-hard optimization problem. Simulations reveal that the proposed approach outperforms existing ones in terms of less delay, higher throughput, and higher satisfaction ratio. The Greedy approach, Two-Stage Relay Selection (TSRS), and the standard A* algorithm were included in the comparative study. The throughput improvements of the proposed scheme are up to 23% and 46.5% compared to TSRS and Greedy, respectively. The proposed scheme possesses the lowest delay and the highest satisfaction rate, among others. With less computational time, the proposed BICA* is more responsive than the standard A* in dynamic environments.
Close-to-Convexity of q-Bessel–Wright Functions
Authors: Din Muhey U. , Raza Mohsan , Xin Qin , Yalçin Sibel , Malik Sarfraz Nawaz
Published: September 2022

In this paper, we aim to find sufficient conditions for the close-to-convexity of q-Bessel–Wright functions with respect to starlike functions, such as z1−z,z1−z2, and −log(1−z) are in the open unit disc. Some consequences related to our main results are also included.