bluuebunny/crossref_metadata_2025 huggingface dataset:
Chronic Diseases, Exercise, and Physical Activity in Childhood: 2016 in Review
Authors: Takken Tim
Published: March 2017

In this year’s review I want to make a case for exercise and physical activity in children with chronic disease or disability. Using two 2016 papers I will illustrate the infancy of the field, especially children who are wheelchair using. More efforts are needed to develop better methods to measure physical activity and exercise capacity in this population. In addition, effective interventions are needed to stimulate a healthy active lifestyle in children with disability. I sincerely hope that the 2016 review will stimulate other researchers to investigate physical activity and exercise in children with chronic disease and disability.
Elite Youth Sports—From Best Pediatric Science Practice To Sports Practice—2016
Authors: Williams Craig A.
Published: March 2017

In my 2015 editorial, I selected two research publications with a focus on an applied sports sciences perspective. This year I have chosen to focus on two publications from a methodological viewpoint, highlighting the importance of laboratory procedures and extraction of data through a systematic review respectively. The first publication by Leites and colleagues (J Appl Physiol) addresses questions in relation to thermoregulation and carbohydrate metabolism in young people. This topic is difficult to conduct due to additional ethical and safety concerns due to exercising in the heat. Nonetheless, there are important basic science questions to be answered. Using a range of measurement techniques including rectal thermometry, 13C-enriched carbohydrate isotopes and procedures to standardize the heat stress equally between a group of men and boys, this project demonstrates an exemplary range of experimental skills. In my second selected paper by Lesinski et al., (Brit J Sports Med), both a systematic review and a meta-analyses were conducted to investigate the dose-response relationships of resistance training on physical performance in youth athletes. As the requirement for more evidence based practice is demanded, the move away from a narrative review to a more methodological and rigorous approach is to be encouraged. It is, in my opinion, a skill that we should be encouraging all our early career pediatric researchers to learn from the outset, the outcome of which can only make our discipline stronger.
Psychosocial Aspects of Youth Physical Activity
Authors: Kipp Lindsay E.
Published: March 2017

Two articles that contribute to the literature on psychosocial predictors of youths’ physical activity motivation and behavior were chosen for commentary. The first article by Fenner and colleagues showed that a family-based intervention was effective at increasing overweight adolescents’ self-determined motivation for physical activity and healthy eating and their quality of life. Significant study contributions include a multidisciplinary team of researchers, multiple pre and post intervention assessments, and a longitudinal test of mechanisms of change. Findings contribute to understanding how to provide overweight adolescents with support and choices at a critical developmental period to ultimately foster lifelong healthy behaviors. The second article by Garn and colleagues examined longitudinal relationships between physical self-perceptions and physical activity among children. Important study contributions include use of accelerometers to assess physical activity and tests of bidirectional relationships. The sample of young children aged 8–11 years also contributes to the literature. Results highlight body acceptance as an important mechanism of focus to foster children’s physical activity behavior. Overall, the highlighted studies show that parental support and positive self-perceptions are important to consider in supporting youths’ active lifestyles.
Antagonism of EGFR and Notch limits resistance to EGFR inhibitors and radiation by decreasing tumor-initiating cell frequency
Authors: Hu Shi , Fu Wenyan , Li Tian , Yuan Qingning , Wang Feifei , Lv Gaojian , Lv Yuanyuan , Fan Xiaoyan , Shen Yafeng , Lin Fangxing , Tang Ying , Ye Xuting , Yang Yongji , Lei Changhai
Published: March 2017

An anti-EGFR/Notch antibody limits acquired resistance to EGFR inhibitors and radiation by reducing tumor-initiating cell frequency.
Deblur Rapidly Resolves Single-Nucleotide Community Sequence Patterns
Authors: Amir Amnon , McDonald Daniel , Navas-Molina Jose A. , Kopylova Evguenia , Morton James T. , Zech Xu Zhenjiang , Kightley Eric P. , Thompson Luke R. , Hyde Embriette R. , Gonzalez Antonio , Knight Rob
Published: March 2017

Deblur provides a rapid and sensitive means to assess ecological patterns driven by differentiation of closely related taxa. This algorithm provides a solution to the problem of identifying real ecological differences between taxa whose amplicons differ by a single base pair, is applicable in an automated fashion to large-scale sequencing data sets, and can integrate sequencing runs collected over time.
Adaptation of Escherichia coli to Long-Term Serial Passage in Complex Medium: Evidence of Parallel Evolution
Authors: Kram Karin E. , Geiger Christopher , Ismail Wazim Mohammed , Lee Heewook , Tang Haixu , Foster Patricia L. , Finkel Steven E.
Published: March 2017

With a growing body of work directed toward understanding the mechanisms of evolution using experimental systems, it is crucial to decipher what effects the experimental setup has on the outcome. If the goal of experimental laboratory evolution is to elucidate underlying evolutionary mechanisms and trends, these must be demonstrated in a variety of systems and environments. Here, we perform experimental evolution in a complex medium allowing the cells to transition through all five phases of growth, including death phase and long-term stationary phase. We show that the swiftness of selection and the specific targets of adaptive evolution are different in this system compared to others. We also observe parallel evolution where different mutations in the same genes are under positive natural selection. Together, these data show that while some outcomes of microbial evolution experiments may be generalizable, many outcomes will be environment or system specific.
Gastric volvulus: a potentially fatal cause of acute abdominal pain
Authors: Sleiwah A , Thomas G , Crawford I , Stanek A
Published: March 2017

A woman aged 67 years attended the emergency department with acute abdominal and back pain of 1-day duration with associated vomiting. The patient had multiorgan failure. Resuscitation was started with intravenous fluids and vasopressors. An abdominal CT scan was completed which confirmed the diagnosis of acute gastric volvulus. The patient was successfully resuscitated from a cardiorespiratory arrest during transfer to the operating theatre. The patient subsequently underwent a total gastrectomy with stapling of the oesophageal and duodenal stumps. The abdomen was packed and left open as a laparostomy with a planned relook 48 hours later was to be performed. Unfortunately, the patient continued to deteriorate postoperatively in the intensive care unit despite maximum organ support for multiorgan failure. A decision was made to withdraw treatment and the patient died 10 hours postoperative. This case illustrates the presentation of acute gastric volvulus at a late stage and the high mortality rate associated with it.
Müllerianosis of the urinary bladder: a rare and problematic bladder tumour
Authors: Patel Amit , Desai Pranali , Malczewski Frances , Stephens Daryl
Published: March 2017

Müllerianosis of the bladder is an extremely rare and highly symptomatic bladder tumour comprising of at least two types of Müllerian derived tissues. We present the case of a 59-year-old woman presenting with painful macroscopic haematuria associated with urgency, frequency and incontinence. She is a nulliparous postmenopausal woman without malignancy risk factors or previous abdominal surgery. Initially identified on ultrasound scan, she was taken for transurethral resection of bladder tumour (TURBT). Histology confirms the diagnosis. Repeat TURBT was required for non-resolution of symptoms. Despite a thorough resection, symptoms recurred within months necessitating partial cystectomy. Although a ‘benign’ lesion, this lesion proved a difficult condition to treat requiring partial cystectomy.
What are the critical elements of sideline screening that can be used to establish the diagnosis of concussion? A systematic review
Authors: Patricios Jon , Fuller Gordon Ward , Ellenbogen Richard , Herring Stanley , Kutcher Jeffrey S , Loosemore Mike , Makdissi Michael , McCrea Michael , Putukian Margot , Schneider Kathryn J
Published: March 2017

Background

Sideline detection is the first and most significant step in recognising a potential concussion and removing an athlete from harm. This systematic review aims to evaluate the critical elements aiding sideline recognition of potential concussions including screening tools, technologies and integrated assessment protocols.

Data sources

Bibliographic databases, grey literature repositories and relevant websites were searched from 1 January 2000 to 30 September 2016. A total of 3562 articles were identified.

Study selection

Original research studies evaluating a sideline tool, technology or protocol for sports-related concussion were eligible, of which 27 studies were included.

Data extraction

A standardised form was used to record information. The QUADAS-2 and Newcastle-Ottawa tools were used to rate risk of bias. Strength of evidence was assessed using the Grades of Recommendation, Assessment, Development and Evaluation Working Group system.

Data synthesis

Studies assessing symptoms, the King-Devick test and multimodal assessments reported high sensitivity and specificity. Evaluations of balance and cognitive tests described lower sensitivity but higher specificity. However, these studies were at high risk of bias and the overall strength of evidence examining sideline screening tools was very low. A strong body of evidence demonstrated that head impact sensors did not provide useful sideline concussion information. Low-strength evidence suggested a multimodal, multitime-based concussion evaluation process incorporating video review was important in the recognition of significant head impact events and delayed onset concussion.

Conclusion

In the absence of definitive evidence confirming the diagnostic accuracy of sideline screening tests, consensus-derived multimodal assessment tools, such as the Sports Concussion Assessment Tool, are recommended. Sideline video review may improve recognition and removal from play of athletes who have sustained significant head impact events. Current evidence does not support the use of impact sensor systems for real-time concussion identification.
Awareness and knowledge about glaucoma and proportion of people with glaucoma in an urban outreach programme in Southeast Nigeria
Authors: Kizor-Akaraiwe Nkiru N , Monye Henrietta I , Okeke Suhanya
Published: March 2017

Aim

The aim of this study was to determine the level of awareness and knowledge of glaucoma and the proportion of people with glaucoma among participants of a 1-day urban eye outreach programme.

Methods

The study was a cross-sectional survey. Data, collected by means of a structured questionnaire, included participants' demographic details, and awareness, knowledge and ocular status as regards glaucoma. Publicity for the outreach was created through the mass media (radio stations and television), churches, handbills, posters and different hospital staff. Data analysis was by Statistical Package for Social SciencesV.20.0. Associations between participants' demographic and other details were tested using Pearson's χ2test, and a p value of <0.05 was considered significant.

Results

Four hundred and seventy-eight (93.2%) of the 513 people in attendance were eligible and consented to participate. Awareness, knowledge and proportion of people with glaucoma in the study population were 65.5%, 36.8% and 14.5%, respectively. Awareness was significantly associated with attainment of some formal education, awareness of a positive family history, and having had an eye check prior to the outreach. The mass media was the most common information source. Good knowledge level was significantly associated with higher educational attainment and occupation, whereas proportion of people with glaucoma was significantly associated with increasing age.

Conclusion

Improving awareness with suitable content for the target population through effective means such as the mass media may be a veritable first step in combating blindness from glaucoma.
Mechanical, biochemical, and dietary determinants of the functional model of bone development of the radius in children and adolescents
Authors: Ludwa Izabella A. , Falk Bareket , Ward Wendy E. , Gammage Kimberley L. , Klentrou Panagiota
Published: March 2017

This study examined the degree to which various mechanical, biochemical, and dietary factors are related to nonweight bearing bone properties in 172 healthy peri-pubertal children (age, 11.7 ± 2.0 years). Dominant radial speed of sound (rSOS) was measured by transaxial quantitative ultrasound at distal radius. Potential modulating factors included somatic maturity offset (years from age of peak height velocity), grip strength, forearm lean cross-sectional area (CSA), physical activity, nutritional intake, and amino-terminal cross-linking propeptide (NTx), reflecting bone resorption. In a hierarchical regression, grip strength adjusted for CSA was the second most important predictor of rSOS after the maturity offset (β = 0.22 and β = 0.33, respectively; R2 = 0.16). When relative grip strength was added to the model the contribution of sex was no longer significant. Calcium intake was a significant predictor of rSOS only after NTx was accounted for in the model (β = 0.17, R2 = 0.21). This may suggest that calcium’s effects on the muscle-bone unit may be modulated through bone resorption. In the final model, relative grip strength together with maturity offset, dietary calcium, and NTx explained up to 21% of the variance in rSOS in this cohort of children. Therefore, during the peri-pubertal stage, size-adjusted forearm muscle strength is related to radial bone strength after controlling for maturity, with calcium intake having a potential indirect association through NTx.
Comparative growth trends of five northern hardwood and montane tree species reveal divergent trajectories and response to climate
Authors: Kosiba Alexandra M. , Schaberg Paul G. , Rayback Shelly A. , Hawley Gary J.
Published: March 2017

In the northeastern United States, tree declines associated with acid deposition induced calcium depletion have been documented, notably for red spruce (Picea rubens Sarg.) and sugar maple (Acer saccharum Marsh.). There is conflicting evidence concerning whether co-occurring tree species capitalized on these declines or suffered similar growth reductions and on how growth has fluctuated relative to environmental variables. We examined five species along three elevational transects on Mt. Mansfield, Vermont: sugar maple, red spruce, red maple (Acer rubrum L.), yellow birch (Betula alleghaniensis Britton), and balsam fir (Abies balsamea (L.) Mill.). We found baseline differences in growth. Red maple and yellow birch had the highest growth, sugar maple and red spruce had intermediate growth, and balsam fir had the lowest growth. While some year-to-year declines were associated with specific stress events, protracted patterns such as recent increases in red spruce and red maple growth were correlated with increased temperature and cooling degree days (heat index). For most species and elevations, there was a positive association between temperature and growth but a negative association with growth in the following year. Based on our comparisons, for some species, growth at Mt. Mansfield aligns with regional trends and suggests that patterns assessed here may be indicative of the broader region.
Dynamical dark energy: Scalar fields and running vacuum
Authors: Solà Joan , Gómez-Valent Adrià , de Cruz Pérez Javier
Published: March 2017

Recent analyses in the literature suggest that the concordance [Formula: see text]CDM model with rigid cosmological term, [Formula: see text] may not be the best description of the cosmic acceleration. The class of “running vacuum models”, in which [Formula: see text] evolves with the Hubble rate, has been shown to fit the string of SNIa + BAO + H(z) + LSS + CMB data significantly better than the [Formula: see text]CDM. Here, we provide further evidence on the time-evolving nature of the dark energy (DE) by fitting the same cosmological data in terms of scalar fields. As a representative model, we use the original Peebles and Ratra potential, [Formula: see text]. We find clear signs of dynamical DE at [Formula: see text] c.l., thus reconfirming through a nontrivial scalar field approach the strong hints formerly found with other models and parametrizations.
Analysis of scalar Dark Matter in a minimal vectorlike Standard Model extension
Authors: Beylin V. , Bezuglov M. , Kuksa V.
Published: March 2017

Vectorlike interaction of additional heavy fermions with the standard bosons is constructed explicitly for the two-color case. Mass splitting in a set of heavy scalar baryons has been analyzed and it turns out that it is small. Relic abundance of the Dark Matter is calculated on the assumption that the lightest neutral scalar baryon is the Dark Matter carrier. The model predicts a strong relation between masses of pseudo-Goldstone states in the conformal limit.
An automatic approach and grip method of micro-particle in 3D space
Authors: Zhang Juan , Wu Wenrong , Bi Lie
Published: March 2017

Micro-particle is hard to be observed as small scale and hard to be gripped as micro-force from substrate, an automatic approach and grip method of micro-particle in the guide of microscopic vision systems is proposed in the paper to grip micro-particle. First, the micro-gripper driven by electrostatic force is introduced and forces in gripping process are analyzed. Second, a micro-assembly robot composed of two microscopic vision systems is established to monitor micro-operation process and to operate micro-particle. Image features of micro-particle and micro-gripper end-effector are extracted by image feature extraction method to calculate relative position of micro-particle and micro-gripper in image space. Last, a movement control strategy in 3D space based on image Jacobian matrix is studied to control micro-gripper approach and align with micro-particle. Experimental results verified the effectiveness of proposed methods.
Investigation of optical properties and local structure of Gd3+ doped nano-crystalline GeSe2
Authors: Hantour Hanan Hassan
Published: March 2017

Pure and Gd-doped nano-crystalline GeSe2 were prepared by the melt-quenching technique. Structure analysis using Rietveld program suggests monoclinic structure for both virgin and doped samples with nano-particle size 41 nm for GeSe2 and 48 nm for Gd-doped sample. A wide optical band gap as estimated from absorbance measurements is 4.1 and 4.8 eV for pure and doped samples in accordance with the confinement effects. Raman spectra show two unresolved components at [Formula: see text]202 cm[Formula: see text] with broad line width. Also, well identified low intensity ([Formula: see text] [Formula: see text] 145 cm[Formula: see text]) and high intensity ([Formula: see text] [Formula: see text] 250 cm[Formula: see text]) bands are detected. For Gd-doped sample, the main band is shifted to lower energies and its full width at half maximum (FWHM) is reduced by [Formula: see text]50% accompanied by an intensity increase of about [Formula: see text]17 fold times. The photoluminescence analysis of the pure sample shows a main emission band at [Formula: see text]604 nm. This band is split into two separated bands with higher intensity. The detected emission bands at wavelength [Formula: see text]650 nm are assigned to transmission from 6G[Formula: see text] to the different 6P[Formula: see text] terms.
The role of dysprosium ions on the physical and optical properties of lithium-borosulfophosphate glasses
Authors: Bulus Ibrahim , Dalhatu S. A. , Hussin R. , Wan Shamsuri W. N. , Yamusa Y. A.
Published: March 2017

Achieving outstanding physical and optical properties of borosulfophosphate glasses via controlled doping of rare earth ions is the key issue in the fabrication of new and highly-efficient glass material for diverse optical applications. Thus, the effect of replacing P2O5 by Dy2O3 on the physical and optical properties of Dy[Formula: see text]-doped lithium-borosulfophosphate glasses with chemical composition of 15Li2O–30B2O3–15SO3–[Formula: see text]P2O5–[Formula: see text]Dy2O3 (where 0.0 mol.% [Formula: see text] mol.%) has been investigated. The glass samples were synthesized from high-purity raw materials via convectional melt-quenching technique and characterized by X-ray diffraction (XRD), energy-dispersive X-ray spectrometry (EDX), density and UV–vis–NIR absorption measurements. The amorphous nature of the prepared glass samples was confirmed by XRD patterns whereas the EDX spectrum depicts elemental traces of O, C, B, S, P and Dy. The physical parameters such as density, refractive index, molar volume, polaron radius and field strength were found to vary nonlinearly with increasing Dy2O3 concentration. UV–vis–NIR absorption spectra revealed seven absorption bands with most dominant peak at 1269 nm (6H[Formula: see text]F[Formula: see text]H[Formula: see text]). From the optical absorption spectra, the optical bandgap and Urbach’s energy have been determined and are related with the structural changes occurring in these glasses with increase in Dy2O3 content. Meanwhile, the bonding parameters ([Formula: see text]) evaluated from the optical absorption spectra were found to be ionic in nature. The superior features exhibited by the current glasses nominate them as potential candidate for nonlinear optical applications.
Long-range effective interactions in a lattice in the semiclassical approximation
Authors: Mamani Evaristo , Calcina-Nogales M. , Sanjinés Diego
Published: March 2017

We consider the semiclassical model of an extended tight-binding Hamiltonian comprising nearest- and next-to-nearest-neighbor interactions for a charged particle hopping in a lattice in the presence of a static arbitrary field and a rapidly oscillating uniform field. The application of Kapitza’s method yields a time-independent effective Hamiltonian with long-range hopping elements that depend on the external static and oscillating fields. Our calculations show that the semiclassical approximation is quite reliable as it yields, for a homogeneous oscillating field, the same effective hopping elements as those derived within the quantum approach. Besides, by controlling the oscillating field, we can engineer the interactions so as to suppress the otherwise dominant interactions (nearest neighbors) and leave as observable effects those due to the otherwise remanent interactions (distant neighbors).
A Model for the Metastability Delay of Sequential Elements
Authors: Polzer Thomas , Steininger Andreas
Published: March 2017

It is well known that every sequential element may become metastable when provided with marginal inputs, such as input transitions occurring too close or input voltage not reaching a defined HI or LO level. In this case the sequential element requires extra time to decide which digital output level to finally present, which is perceived as an output delay. The amount of this delay depends on how close the element’s state is to the balance point, at which the delay may, theoretically, become infinite. While metastability can be safely avoided within a closed timing domain, it cannot be completely ruled out at timing domain boundaries. Therefore it is important to quantify its effect. Traditionally this is done by means of a “mean time between upsets” (MTBU) which gives the expected interval between two metastable upsets. The latter is defined as the event of latching the still undecided output of one sequential element by a subsequent one. However, such a definition only makes sense in a time-safe environment like a synchronous design. In this paper we will extend the scope to so-called value-safe environments, in which a sequential element can safely finalize its decision, since the subsequent one waits for completion before capturing its output. Here metastability is not a matter of “failure” but a performance issue, and hence characterization by MTBU is not intuitive. Therefore we will put the focus on the delay aspect and derive a suitable model. This model extends existing approaches by also including the area of very weak metastability and thus providing complete coverage. We will show its validity through comparison with transistor-level simulation results for the most popular sequential elements in different implementations, point out its relation to the traditional MTBU model parameters, namely [Formula: see text] and [Formula: see text], and show how to use it for calculating the performance penalty in a value-safe environment.
Fractional quantum integral operator with general kernels and applications
Authors: Babakhani Azizollah , Neamaty Abdolali , Yadollahzadeh Milad , Agahi Hamzeh
Published: March 2017

In this paper, we first introduce the concept of fractional quantum integral with general kernels, which generalizes several types of fractional integrals known from the literature. Then we give more general versions of some integral inequalities for this operator, thus generalizing some previous results obtained by many researchers.2,8,25,29,30,36
Two Ramanujan’s formulas and normal distribution
Authors: Wang Mingjin
Published: March 2017

In this paper, we point out the relationship between the two Ramanujan’s formulas for Beta integrals and normal distribution. Some of the applications are also given.
Igneous activity in the Bornu Basin, onshore NE Nigeria; implications for opening of the South Atlantic
Authors: Suleiman A. A. , Magee C. , Jackson C. A.-L. , Fraser A. J.
Published: March 2017

The structure of igneous plumbing systems in circum-South Atlantic, intra-continental rift basins (e.g. the West and Central African Rift Systems) remains enigmatic owing to poor subsurface data coverage and quality. How magmatism in these basins related to the opening of the South Atlantic is thus poorly understood. We integrate 2D and 3D seismic reflection data (
c.
27 600 km
2
), data from 23 boreholes, and field observations from the Bornu Basin and Upper Benue Trough, onshore NE Nigeria, to examine the timing and development of igneous bodies possibly related to opening of the South Atlantic. We identify numerous sills, which typically have saucer-shaped and en echelon morphologies, and extrusive volcanic cones. The igneous rocks are alkali basalts and dolerites. Seismic-stratigraphic relationships indicate that emplacement occurred in the Early Cretaceous (Albian to Cenomanian;
c.
120 Ma), Late Cretaceous (Santonian to early Campanian;
c.
83 Ma) and Cenozoic (Miocene;
c.
22 Ma). Magmatism was broadly coeval with major plate boundary interactions, characterized by major azimuthal changes in fracture zones in the developing South Atlantic Ocean. The broad temporal correlation between intra-continental rift basin magmatism and plate boundary interactions suggests that periods of magma emplacement may have, in some way, been instigated by stress dissipation into intra-continental rift basins.
Comprehensive functional screening of miRNAs involved in fat cell insulin sensitivity among women
Authors: Dahlman Ingrid , Belarbi Yasmina , Laurencikiene Jurga , Pettersson Annie M. , Arner Peter , Kulyté Agné
Published: March 2017

The key pathological link between obesity and type 2 diabetes is insulin resistance, but the molecular mechanisms are not entirely identified. micro-RNAs (miRNA) are dysregulated in obesity and may contribute to insulin resistance. Our objective was to detect and functionally investigate miRNAs linked to insulin sensitivity in human subcutaneous white adipose tissue (scWAT). Subjects were selected based on the insulin-stimulated lipogenesis response of subcutaneous adipocytes. Global miRNA profiling was performed in abdominal scWAT of 18 obese insulin-resistance (OIR), 21 obese insulin-sensitive (OIS), and 9 lean women. miRNAs demonstrating differential expression between OIR and OIS women were overexpressed in human in vitro-differentiated adipocytes followed by assessment of lipogenesis and identification of miRNA targets by measuring mRNA/protein expression and 3′-untranslated region analysis. Eleven miRNAs displayed differential expression between OIR and OIS states. Overexpression of miR-143-3p and miR-652-3p increased insulin-stimulated lipogenesis in human in vitro differentiated adipocytes and directly or indirectly affected several genes/proteins involved in insulin signaling at transcriptional or posttranscriptional levels. Adipose expression of miR-143-3p and miR-652-3p was positively associated with insulin-stimulated lipogenesis in scWAT independent of body mass index. In conclusion, miR-143-3p and miR-652-3p are linked to scWAT insulin resistance independent of obesity and influence insulin-stimulated lipogenesis by interacting at different steps with insulin-signaling pathways.
Skeletal muscle PGC-1β signaling is sufficient to drive an endurance exercise phenotype and to counteract components of detraining in mice
Authors: Lee Samuel , Leone Teresa C. , Rogosa Lisa , Rumsey John , Ayala Julio , Coen Paul M. , Fitts Robert H. , Vega Rick B. , Kelly Daniel P.
Published: March 2017

Peroxisome proliferator-activated receptor-γ coactivator (PGC)-1α and -1β serve as master transcriptional regulators of muscle mitochondrial functional capacity and are capable of enhancing muscle endurance when overexpressed in mice. We sought to determine whether muscle-specific transgenic overexpression of PGC-1β affects the detraining response following endurance training. First, we established and validated a mouse exercise-training-detraining protocol. Second, using multiple physiological and gene expression end points, we found that PGC-1β overexpression in skeletal muscle of sedentary mice fully recapitulated the training response. Lastly, PGC-1β overexpression during the detraining period resulted in partial prevention of the detraining response. Specifically, an increase in the plateau at which O2uptake (V̇o2) did not change from baseline with increasing treadmill speed [peak V̇o2(ΔV̇o2max)] was maintained in trained mice with PGC-1β overexpression in muscle 6 wk after cessation of training. However, other detraining responses, including changes in running performance and in situ half relaxation time (a measure of contractility), were not affected by PGC-1β overexpression. We conclude that while activation of muscle PGC-1β is sufficient to drive the complete endurance phenotype in sedentary mice, it only partially prevents the detraining response following exercise training, suggesting that the process of endurance detraining involves mechanisms beyond the reversal of muscle autonomous mechanisms involved in endurance fitness. In addition, the protocol described here should be useful for assessing early-stage proof-of-concept interventions in preclinical models of muscle disuse atrophy.
Increased ERp57 Expression in HBV-Related Hepatocellular Carcinoma: Possible Correlation and Prognosis
Authors: Liu Miao , Du Lingyao , He Zhiliang , Yan Libo , Shi Ying , Shang Jin , Tang Hong
Published: March 2017

Aim.ERp57 is involved in virus induced endoplasmic reticulum stress (ERS) and plays an important role in tumorigenesis. This study aimed to find whether HBV infection altered ERp57 expression and whether ERp57 regulation was involved in hepatitis B virus-related hepatocellular carcinoma (HBV-HCC) genesis.Materials and Methods.HBV-HCC tissues, chronic hepatitis B (CHB) liver tissues, and normal liver tissues were acquired. ERp57 expressions in these tissues were detected through immunohistochemistry (IHC). And ERp57 expression in liver cell line L02, HBV replicative liver cell line L02-pHBV4.1, and HCC cell lines were detected through western blot for verification. Then medical data on patients providing HCC tissues were collected and analyzed along with ERp57 expression.Results.Higher ERp57 expression was found in HCC and CHB tissues (p<0.001). And HCC cell lines and L02-pHBV4.1 presented higher ERp57 expression as well. In patients, ERp57 expression showed significant differences between death and survival groups (p=0.037). And cumulative survival in patients with higher ERp57 (score⩾8.75) is significantly lower (p=0.009).Conclusion.Our study found increased expression of ERp57 in HBV-HCC. Such altered expression could be related to HBV infection and high ERp57 expression may lead to poor prognosis of HBV-HCC patients.
Leflunomide in the Treatment of a Pseudotumoral Genital Herpes Simplex Virus Infection in an HIV Patient
Authors: Roger Marie R. , Anstead Gregory M.
Published: March 2017

The patient is a 52-year-old African American man with a past medical history of HIV infection (on antiretroviral therapy, CD4 count 399 cells/µL, and undetectable HIV viral load) and recurrent genital herpes. While on valacyclovir, the patient presented with four tumorous lesions on the perineum and scrotum. A biopsy specimen stained positively with HSV-1 and HSV-2 immunostains and displayed a lymphoplasmacytic infiltrate. The patient received foscarnet and imiquimod for two weeks with minimal improvement. Based on the previous activity of leflunomide, which has both antiviral and immunomodulatory properties, in cytomegalovirus and herpes simplex infections, leflunomide 20 mg orally twice daily was started. The patient received 23 days of foscarnet, 14 days of topical imiquimod, and 11 days of leflunomide with approximately 80% reduction in the size of the perineal lesion. After nine months on leflunomide there was complete regression of the large perineal lesion and only two small ulcerations remained on the scrotum. Pseudotumoral herpes lesions in HIV patients represent an immune reconstitution event and are poorly responsive to the usual anti-herpes agents. This report demonstrates the successful use of leflunomide in the treatment of an HIV patient with pseudotumoral herpes. Thalidomide has also been used with some success.
Treatment and Patient Reported Outcome in Children with Hirschsprung Disease and Concomitant Congenital Heart Disease
Authors: Hasserius Johan , Hedbys Josefine , Graneli Christina , Hagelsteen Kristine , Stenström Pernilla
Published: March 2017

Purpose. Congenital heart disease (CHD) is reported to be associated with Hirschsprung disease (HD). The aim was to evaluate any differences between children with HD with and without CHD, respectively, with regard to patient characteristics, medical care, and patient reported bowel function. Method. This is a retrospective chart study and a cross-sectional long-term follow-up of patients older than 4 years old, including all children with HD operated on with transanal endorectal pull-through (TERPT) at a tertiary center of pediatric surgery. Information about patient characteristics, diagnostics, surgery, and medical care was compiled. At long-term follow-up, bowel function was assessed by Bowel Function Score. Results. Included were 53 HD-patients, 13 with CHD and 40 without CHD. Children with CHD more commonly presented with failure to thrive; 4 (23%) compared to those without CHD (0%) (p<0.01). In the long-term follow-up, including 32 patients (6 with CHD), constipation was more commonly reported by children with CHD 5 (83%) than by children without CHD 4 (27%) (p=0.01). No differences were shown in the other parameters such as fecal control and incontinence. Conclusion. HD-patients with CHD more commonly presented with failure to thrive and more frequently reported constipation than HD-patients without CHD. The findings indicate that HD-patients with CHD might need special consideration in their initial care and long-term follow-up.
Learning Curve Analysis of Different Stages of Robotic-Assisted Laparoscopic Hysterectomy
Authors: Tang Feng-Hsiang , Tsai Eing-Mei
Published: March 2017

Objective. To analyze the learning curves of the different stages of robotic-assisted laparoscopic hysterectomy. Design. Retrospective analysis. Design Classification. Canadian Task Force classification II-2. Setting. Kaohsiung Medical University Hospital, Kaohsiung, Taiwan. Patient Intervention. Women receiving robotic-assisted total and subtotal laparoscopic hysterectomies for benign conditions from May 1, 2013, to August 31, 2015. Measurements and Main Results. The mean age, body mass index (BMI), and uterine weight were 46.44±5.31 years, 23.97±4.75 kg/m2, and 435.48±250.62 g, respectively. The most rapid learning curve was obtained for the main surgery console stage; eight experiences were required to achieve duration stability, and the time spent in this stage did not violate the control rules. The docking stage required 14 experiences to achieve duration stability, and the suture stage was the most difficult to master, requiring 26 experiences. BMI did not considerably affect the duration of the three stages. The uterine weight and the presence of adhesion did not substantially affect the main surgery console time. Conclusion. Different stages of robotic-assisted laparoscopic hysterectomy have different learning curves. The main surgery console stage has the most rapid learning curve, whereas the suture stage has the slowest learning curve.
Terahertz Spectroscopy and Brewster Angle Reflection Imaging of Acoustic Tiles
Authors: Kilcullen Patrick , Shegelski Mark , Na MengXing , Purschke David , Hegmann Frank , Reid Matthew
Published: March 2017

A Brewster angle reflection imaging apparatus is demonstrated which is capable of detecting hidden water-filled voids in a rubber tile sample. This imaging application simulates a real-world hull inspection problem for Royal Canadian Navy Victoria-class submarines. The tile samples represent a challenging imaging application due to their large refractive index and absorption coefficient. With a rubber transmission window at approximately 80 GHz, terahertz (THz) sensing methods have shown promise for probing these structures in the laboratory. Operating at Brewster’s angle allows for the typically strong front surface reflection to be minimized while also conveniently making the method insensitive to air-filled voids. Using a broadband THz time-domain waveform imaging system (THz-TDS), we demonstrate satisfactory imaging and detection of water-filled voids without complicated signal processing. Optical properties of the tile samples at low THz frequencies are also reported.
Investigation on Durability Performance in Early Aged High-Performance Concrete Containing GGBFS and FA
Authors: Jang Seung-Yup , Karthick Subbiah , Kwon Seung-Jun
Published: March 2017

The significance of concrete durability increases since RC (Reinforced Concrete) structures undergo degradation due to aggressive environmental conditions, which affects structural safety and serviceability. Steel corrosion is the major cause for the unexpected failure of RC structures. The main cause for the corrosion initiation is the ingress of chloride ions prevailing in the environment. Hence quantitative evaluation of chloride diffusion becomes very important to obtain a chloride diffusion coefficient and resistance to chloride ion intrusion. In the present investigation, 15 mix proportions with 3 water-to-binder ratios (0.37, 0.42, and 0.47) and 3 replacement ratios (0, 30, and 50%) were prepared for HPC (high-performance concrete) with fly-ash and ground granulated blast furnace slag. Chloride diffusion coefficient was measured under nonstationary condition. In order to evaluate the microstructure characteristics, porosity through MIP was also measured. The results of compressive strength, chloride diffusion, and porosity are compared with electrical charges. This paper deals with the results of the concrete samples exposed for only 2 months, but it is a part of the total test plan for 100 years. From the work, time-dependent diffusion coefficients in HPC and the key parameters for durability design are proposed.
Mechanisms Regulating Stemness and Differentiation in Embryonal Carcinoma Cells
Authors: Kelly Gregory M. , Gatie Mohamed I.
Published: March 2017

Just over ten years have passed since the seminal Takahashi-Yamanaka paper, and while most attention nowadays is on induced, embryonic, and cancer stem cells, much of the pioneering work arose from studies with embryonal carcinoma cells (ECCs) derived from teratocarcinomas. This original work was broad in scope, but eventually led the way for us to focus on the components involved in the gene regulation of stemness and differentiation. As the name implies, ECCs are malignant in nature, yet maintain the ability to differentiate into the 3 germ layers and extraembryonic tissues, as well as behave normally when reintroduced into a healthy blastocyst. Retinoic acid signaling has been thoroughly interrogated in ECCs, especially in the F9 and P19 murine cell models, and while we have touched on this aspect, this review purposely highlights how some key transcription factors regulate pluripotency and cell stemness prior to this signaling. Another major focus is on the epigenetic regulation of ECCs and stem cells, and, towards that end, this review closes on what we see as a new frontier in combating aging and human disease, namely, how cellular metabolism shapes the epigenetic landscape and hence the pluripotency of all stem cells.
Clinical Results and Serum Metal Ion Concentrations following Ceramic-on-Metal Total Hip Arthroplasty at a Mean Follow-Up of 60 Months
Authors: Maurer-Ertl W. , Pranckh-Matzke D. , Friesenbichler J. , Bratschitsch G. , Holzer L. A. , Maier M. , Leithner A.
Published: March 2017

Background. Increased metal ion levels following total hip arthroplasty (THA) with metal-on-metal bearings are a highly debated topic. Local soft tissue reactions with chronic pain and systemic side effects such as neuropathy are described. The aim of the current study was to determine the serum metal ion concentrations of Cobalt (Co) and Chrome (Cr) after THA with a ceramic-on-metal (CoM) bearing. Patients and Methods. Between 2008 and 2010, 20 patients underwent THA using a CoM bearing. Clinical function was evaluated by standardized scores systems (Harris Hip Score and WOMAC Score) and radiological examination included X-rays. Patient’s blood samples were obtained for metal ion analysis and correlation analysis was done between these results and implant position. Results. Overall, 13 patients with 14 CoM devices were available for the current series. The mean age at time of surgery was 61 years (range, 41 to 85). The postoperative follow-up ranged from 49 to 68 months (mean, 58). Metal ion determination showed mean concentrations of 3,1 µg/L (range, 0,3–15,2 µg/L) for Co and 1,6 µg/L (range, 0,1–5,5 µg/L) for Cr, respectively. A correlation between cup anteversion and Co and Cr concentrations was shown. Conclusion. The current series showed increments for Co and Cr following CoM THA. However, these levels are lower compared to metal ion concentrations in patients with metal-on-metal bearings and the international accepted threshold for revision of MoM devices. We recommend routine follow-up including at least one obligatory evaluation of serum metal ion concentrations and an MRI once to exclude local soft tissue reactions.
SOCS1 Regulates Apoptosis and Inflammation by Inhibiting IL-4 Signaling in IL-1β-Stimulated Human Osteoarthritic Chondrocytes
Authors: He Qiang , Sun Caihong , Lei Wei , Ma Jianbing
Published: March 2017

Recently, Suppressor of Cytokine Signaling 1 (SOCS1) was identified as a potential therapeutic target for osteoarthritis (OA) treatment. However, the mechanisms and signaling pathways of SOCS1 in the regulation of OA development are unclear. The purpose of the current study was to investigate whether interleukin- (IL-) 4 was involved in regulatory mechanism of SOCS1 in human osteoarthritic chondrocytes. First, IL-1βwas used to stimulate human osteoarthritic chondrocytes isolated from the articular cartilage of OA patients undergoing total knee replacement. The protein and mRNA expression levels of SOCS1 were upregulated in IL-1β-stimulated human osteoarthritic chondrocytes compared with control cells. The knockdown of SOCS1 increased cell viability and inhibited cell apoptosis. It was also found that IL-4 expression was increased by SOCS1 silencing. Additionally, knockdown of IL-4 reduced cell viability and increased cell apoptosis of osteoarthritic chondrocytes transfected with SOCS1 siRNA. Moreover, the decreased expression of inflammatory factors induced by SOCS1 was enhanced by IL-4 knockdown. In conclusion, IL-4 signaling plays a crucial role in the regulatory functions of SOCS1 in apoptosis and inflammation in human osteoarthritic chondrocytes. These findings provide a potential therapeutic target for the clinical treatment of OA.
The Distribution of Phosphatidylcholine Species in Superficial-Type Pharyngeal Carcinoma
Authors: Ishikawa Seiji , Tateya Ichiro , Hayasaka Takahiro , Shinriki Satoru , Masaki Noritaka , Hirano Shigeru , Kitamura Morimasa , Muto Manabu , Morita Shuko , Setou Mitsutoshi , Ito Juichi
Published: March 2017

Objectives. Superficial-type pharyngeal squamous cell carcinoma (STPSCC) is defined as carcinoma in situ or microinvasive squamous cell carcinoma without invasion to the muscular layer. An exploration of the biological characteristics of STPSCC could uncover the invasion mechanism of this carcinoma. Phosphatidylcholine (PC) in combination with fatty acids is considered to play an important role in cell motility. Imaging mass spectrometry (IMS) is especially suitable for phospholipid analysis because this technique can distinguish even fatty acid compositions. Study Design. IMS analysis of frozen human specimens. Methods. IMS analysis was conducted to elucidate the distribution of PC species in STPSCC tissues. STPSCC tissue sections from five patients were analyzed, and we identified the signals that showed significant increases in the subepithelial invasive region relative to the superficial region. Results. Three kinds of PC species containing arachidonic acid, that is, PC (16:0/20:4), PC (18:1/20:4), and PC (18:0/20:4), were increased in the subepithelial invasive region. Conclusion. These results may be associated with the invasion mechanism of hypopharyngeal carcinoma.
Numerical Investigation of Damage Risks of Roadway Surrounding Rocks under Oblique Incident Dynamic Loads
Authors: Wang Zhengyi , Dou Linming , Li Jing , Kang Kai , Feng Longfei
Published: March 2017

In order to quantitatively demonstrate the damage risks of roadway surrounding rocks and meanwhile comprehensively consider stress states of surrounding rocks, an index to evaluate damage hazards was established based on Mohr-Coulomb strength criterion. Taking the track roadway of Longwall Panel 402103 in Hujiahe Coal Mine as an example, damage risks and dynamic response characteristics of surrounding rocks under oblique incident dynamic loads were studied in accordance with the new evaluation criterion. The results show that all conventional analysis indexes only reflect the stress distribution characteristics of coal-rocks from a certain aspect in two-dimension space, while all stress components can be considered by the damage risk index, with the evaluation criterion quantified, which can comprehensively reveal the stress states and damage of coal-rock masses. Under dynamic loading, the overall damage risks and damage degrees of roadway surrounding rocks are attributed to the superposition results of dynamic and static loads. The dynamic loads not only aggravate the damage of shallow surrounding rocks but also increase the damage risks of deep parts. The engineering practice demonstrates that the analysis results coincide with in situ damage of roadway surrounding rocks.