bluuebunny/crossref_metadata_2025 huggingface dataset:
Development of Dapagliflozin Solid Lipid Nanoparticles as a Novel Carrier for Oral Delivery: Statistical Design, Optimization, In-Vitro and In-Vivo Characterization, and Evaluation
Authors: Unnisa Aziz , Chettupalli Ananda K. , Al Hagbani Turki , Khalid Mohammad , Jandrajupalli Suresh B. , Chandolu Swarnalatha , Hussain Talib
Published: May 2022

Controlling hyperglycemia and avoiding glucose reabsorption are significant goals in type 2 diabetes treatments. Among the numerous modes of medication administration, the oral route is the most common. Introduction: Dapagliflozin is an oral hypoglycemic agent and a powerful, competitive, reversible, highly selective, and orally active human SGLT2 inhibitor. Dapagliflozin-loaded solid lipid nanoparticles (SLNs) are the focus of our present investigation. Controlled-release lipid nanocarriers were formulated by integrating them into lipid nanocarriers. The nanoparticle size and lipid utilized for formulation help to regulate the release of pharmaceuticals over some time. Dapagliflozin-loaded nanoparticles were formulated by hot homogenization followed by ultra-sonication. The morphology and physicochemical properties of dapagliflozin-SLNs have been characterized using various techniques. The optimized dapagliflozin-SLNs have a particle size ranging from 100.13 ± 7.2 to 399.08 ± 2.4 nm with 68.26 ± 0.2 to 94.46 ± 0.7% entrapment efficiency (%EE). Dapagliflozin-SLNs were optimized using a three-factor, three-level Box–Behnken design (BBD). Polymer concentration (X1), surfactant concentration (X2), and stirring duration (X3) were chosen as independent factors, whereas %EE, cumulative drug release (%CDR), and particle size were selected as dependent variables. Interactions between drug substances and polymers were studied using Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Differential scanning calorimetry (DSC), X-ray diffraction (XRD), and atomic force microscopy (AFM) analysis indicated the crystalline change from the drug to the amorphous crystal. Electron microscope studies revealed that the SLNs’ structure is nearly perfectly round. It is evident from the findings that dapagliflozin-SLNs could lower elevated blood glucose levels to normal in STZ-induced diabetic rats, demonstrating a better hypoglycemic impact on type 2 diabetic patients. The in vivo pharmacokinetic parameters of SLNs exhibited a significant rise in Cmax (1258.37 ± 1.21 mcg/mL), AUC (5247.04 mcg/mL), and oral absorption (2-fold) of the drug compared to the marketed formulation in the Sprague Dawley rats.
The Antitumoral/Antimetastatic Action of the Flavonoid Brachydin A in Metastatic Prostate Tumor Spheroids In Vitro Is Mediated by (Parthanatos) PARP-Related Cell Death
Authors: Ribeiro Diego Luis , Tuttis Katiuska , Oliveira Larissa Cristina Bastos de , Serpeloni Juliana Mara , Gomes Izabela Natalia Faria , Lengert André van Helvoort , Rocha Cláudia Quintino da , Reis Rui Manuel , Cólus Ilce Mara de Syllos , Antunes Lusânia Maria Greggi
Published: May 2022

Metastatic prostate cancer (mPCa) is resistant to several chemotherapeutic agents. Brachydin A (BrA), a glycosylated flavonoid extracted from Fridericia platyphylla, displays a remarkable antitumoral effect against in vitro mPCa cells cultured as bidimensional (2D) monolayers. Considering that three-dimensional (3D) cell cultures provide a more accurate response to chemotherapeutic agents, this study investigated the antiproliferative/antimetastatic effects of BrA and the molecular mechanisms underlying its action in mPCa spheroids (DU145) in vitro. BrA at 60–100 μM was cytotoxic, altered spheroid morphology/volume, and suppressed cell migration and tumor invasiveness. High-content analysis revealed that BrA (60–100 µM) reduced mitochondrial membrane potential and increased apoptosis and necrosis markers, indicating that it triggered cell death mechanisms. Molecular analysis showed that (i) 24-h treatment with BrA (80–100 µM) increased the protein levels of DNA disruption markers (cleaved-PARP and p-γ-H2AX) as well as decreased the protein levels of anti/pro-apoptotic (BCL-2, BAD, and RIP3K) and cell survival markers (p-AKT1 and p-44/42 MAPK); (ii) 72-h treatment with BrA increased the protein levels of effector caspases (CASP3, CASP7, and CASP8) and inflammation markers (NF-kB and TNF-α). Altogether, our results suggest that PARP-mediated cell death (parthanatos) is a potential mechanism of action. In conclusion, BrA confirms its potential as a candidate drug for preclinical studies against mPCa.
Ciprofloxacin in Patients Undergoing Extracorporeal Membrane Oxygenation (ECMO): A Population Pharmacokinetic Study
Authors: Alihodzic Dzenefa , Wicha Sebastian G. , Frey Otto R. , König Christina , Baehr Michael , Jarczak Dominik , Kluge Stefan , Langebrake Claudia
Published: May 2022

Extracorporeal membrane oxygenation (ECMO) is utilized to temporarily sustain respiratory and/or cardiac function in critically ill patients. Ciprofloxacin is used to treat nosocomial infections, but data describing the effect of ECMO on its pharmacokinetics is lacking. Therefore, a prospective, observational trial including critically ill adults (n = 17), treated with ciprofloxacin (400 mg 8–12 hourly) during ECMO, was performed. Serial blood samples were collected to determine ciprofloxacin concentrations to assess their pharmacokinetics. The pharmacometric modeling was performed (NONMEM®) and utilized for simulations to evaluate the probability of target attainment (PTA) to achieve an AUC0–24/MIC of 125 mg·h/L for ciprofloxacin. A two-compartment model most adequately described the concentration-time data of ciprofloxacin. Significant covariates on ciprofloxacin clearance (CL) were plasma bicarbonate and the estimated glomerular filtration rate (eGFR). For pathogens with an MIC of ≤0.25 mg/L, a PTA of ≥90% was attained. However, for pathogens with an MIC of ≥0.5 mg/L, plasma bicarbonate ≥ 22 mmol/L or eGFR ≥ 10 mL/min PTA decreased below 90%, steadily declining to 7.3% (plasma bicarbonate 39 mmol/L) and 21.4% (eGFR 150 mL/min), respectively. To reach PTAs of ≥90% for pathogens with MICs ≥ 0.5 mg/L, optimized dosing regimens may be required.
Essential Oils of Gardenia jasminoides J. Ellis and Gardenia jasminoides f. longicarpa Z.W. Xie & M. Okada Flowers: Chemical Characterization and Assessment of Anti-Inflammatory Effects in Alveolar Macrophage
Authors: Zhang Nan , Bian Ying , Yao Lei
Published: May 2022

Alveolar macrophage is the predominant cell type in the lung and is thought to be the major target for anti-inflammatory therapy in chronic obstructive pulmonary disease (COPD). Aromatherapy using natural essential oils with anti-inflammatory effects for inhalable administration is a potential complementary and alternative therapy for COPD treatment. The Gardenia jasminoides flower is famous for its fragrance in East Asia and is used for treating colds and lung problems in folk medicine. Therefore, in the present study, flower essential oils from two main medicinal gardenia varieties (G. jasminoides J. Ellis and G. jasminoides f. longicarpa Z.W. Xie & M. Okada) were extracted by hydro-distillation, and their chemical components were analyzed by GC-MS. The anti-inflammatory effects of the two essential oils and their main ingredients were further studied on lipopolysaccharide (LPS)-induced models in murine alveolar macrophages (MH-S). The results indicated that the chemical constituents of the two gardenia varieties were quite different. Alcohol accounted for 53.8% of the G. jasminoides essential oil, followed by terpenes (16.01%). Terpenes accounted for 34.32% of the G. jasminoides f. longicarpa essential oil, followed by alcohols (19.6%) and esters (13.85%). Both the two gardenia essential oils inhibited the LPS-induced nitric oxide (NO) release and reduced the production of tumor necrosis factor-α (TNF-α) and prostaglandin E2 (PGE2) in the MH-S cells. Linalool and α-farnesene dose-dependently reduced the NO release in the MH-S cells. Linalool and α-farnesene did not affect the PGE2 production but regulated the expression of TNF- α. In addition to linalool and α-farnesene, other components in the gardenia flower essential oils appeared to be able to act as anti-inflammatory agents and influence the PGE2 pathway.
Anti-Virulence Activity of 3,3′-Diindolylmethane (DIM): A Bioactive Cruciferous Phytochemical with Accelerated Wound Healing Benefits
Authors: Golberg Karina , Markus Victor , Kagan Bat-el , Barzanizan Sigalit , Yaniv Karin , Teralı Kerem , Kramarsky-Winter Esti , Marks Robert S. , Kushmaro Ariel
Published: May 2022

Antimicrobial resistance is among the top global health problems with antibacterial resistance currently representing the major threat both in terms of occurrence and complexity. One reason current treatments of bacterial diseases are ineffective is the occurrence of protective and resistant biofilm structures. Phytochemicals are currently being reviewed for newer anti-virulence agents. In the present study, we aimed to investigate the anti-virulence activity of 3,3′-diindolylmethane (DIM), a bioactive cruciferous phytochemical. Using a series of in vitro assays on major Gram-negative pathogens, including transcriptomic analysis, and in vivo porcine wound studies as well as in silico experiments, we show that DIM has anti-biofilm activity. Following DIM treatment, our findings show that biofilm formation of two of the most prioritized bacterial pathogens Acinetobacter baumannii and Pseudomonas aeruginosa was inhibited respectively by 65% and 70%. Combining the antibiotic tobramycin with DIM enabled a high inhibition (94%) of P. aeruginosa biofilm. A DIM-based formulation, evaluated for its wound-healing efficacy on P. aeruginosa-infected wounds, showed a reduction in its bacterial bioburden, and wound size. RNA-seq was used to evaluate the molecular mechanism underlying the bacterial response to DIM. The gene expression profile encompassed shifts in virulence and biofilm-associated genes. A network regulation analysis showed the downregulation of 14 virulence-associated super-regulators. Quantitative real-time PCR verified and supported the transcriptomic results. Molecular docking and interaction profiling indicate that DIM can be accommodated in the autoinducer- or DNA-binding pockets of the virulence regulators making multiple non-covalent interactions with the key residues that are involved in ligand binding. DIM treatment prevented biofilm formation and destroyed existing biofilm without affecting microbial death rates. This study provides evidence for bacterial virulence attenuation by DIM.
The Potential Anti-Photoaging Effect of Photodynamic Therapy Using Chlorin e6-Curcumin Conjugate in UVB-Irradiated Fibroblasts and Hairless Mice
Authors: Hur Ga-Hee , Ryu A-Reum , Kim Yong-Wan , Lee Mi-Young
Published: May 2022

Photodynamic therapy (PDT) has been used to treat cancers and non-malignant skin diseases. In this study, a chlorin e6–curcumin conjugate (Ce6-PEG-Cur), a combination of chlorin e6 (Ce6) and curcumin via a PEG linker, was used as a photosensitizer. The in vitro and in vivo effects of PDT using Ce6-PEG-Cur were analyzed in UVB-irradiated fibroblasts and hairless mice. The UVB-induced expression of MMPs was reduced in Hs68 fibroblast cells, and procollagen type Ⅰ expression was enhanced by Ce6-PEG-Cur-mediated PDT on a Western blotting gel. Moreover, UVB-induced collagen levels were restored upon application of Ce6-PEG-Cur-mediated PDT. Ce6-PEG-Cur-mediated PDT inhibited the expression of phosphorylated p38 in the MAPK signaling pathway, and it reduced the expression of phosphorylated NF-κB. In animal models, Ce6-PEG-Cur-mediated PDT inhibited the expression of MMPs, whereas procollagen type Ⅰ levels were enhanced in the dorsal skin of UVB-irradiated mice. Moreover, UVB-induced dorsal roughness was significantly reduced following Ce6-PEG-Cur-mediated PDT treatment. H&E staining and Masson’s trichrome staining showed that the thickness of the epidermal region was reduced, and the density of collagen fibers increased. Taken together, Ce6-PEG-Cur-mediated PDT might delay and improve skin photoaging by ultraviolet light, suggesting its potential for use as a more effective photo-aging treatment.
Toxicity Assessment of Mesoporous Silica Nanoparticles upon Intravenous Injection in Mice: Implications for Drug Delivery
Authors: MacCuaig William M. , Samykutty Abhilash , Foote Jeremy , Luo Wenyi , Filatenkov Alexander , Li Min , Houchen Courtney , Grizzle William E. , McNally Lacey R.
Published: May 2022

Nanoparticles are popular tools utilized to selectively deliver drugs and contrast agents for identification and treatment of disease. To determine the usefulness and translational potential of mesoporous silica nanoparticles (MSNs), further evaluations of toxicity are required. MSNs are among the most utilized nano-delivery systems due to ease of synthesis, pore structure, and functionalization. This study aims to elucidate toxicity as a result of intravenous injection of 25 nm MSNs coated with chitosan (C) or polyethylene glycol (PEG) in mice. Following acute and chronic injections, blood was evaluated for standard blood chemistry and complete blood count analyses. Blood chemistry results primarily indicated that no abnormalities were present following acute or chronic injections of MSNs, or C/PEG-coated MSNs. After four weekly administered treatments, vital organs showed minor exacerbation of pre-existing lesions in the 35KPEG-MSN and moderate exacerbation of pre-existing lesions in uncoated MSN and 2KPEG-MSN treatment groups. In contrast, C-MSN treatment groups had minimal changes compared to controls. This study suggests 25 nm MSNs coated with chitosan should elicit minimal toxicity when administered as either single or multiple intravenous injections, but MSNs coated with PEG, especially 2KPEG may exacerbate pre-existing vascular conditions. Further studies should evaluate varying sizes and types of nanoparticles to provide a better overall understanding on the relation between nanoparticles and in vivo toxicity.
A Single-Cell Network-Based Drug Repositioning Strategy for Post-COVID-19 Pulmonary Fibrosis
Authors: Li Albert , Chen Jhih-Yu , Hsu Chia-Lang , Oyang Yen-Jen , Huang Hsuan-Cheng , Juan Hsueh-Fen
Published: May 2022

Post-COVID-19 pulmonary fibrosis (PCPF) is a long-term complication that appears in some COVID-19 survivors. However, there are currently limited options for treating PCPF patients. To address this problem, we investigated COVID-19 patients’ transcriptome at single-cell resolution and combined biological network analyses to repurpose the drugs treating PCPF. We revealed a novel gene signature of PCPF. The signature is functionally associated with the viral infection and lung fibrosis. Further, the signature has good performance in diagnosing and assessing pulmonary fibrosis. Next, we applied a network-based drug repurposing method to explore novel treatments for PCPF. By quantifying the proximity between the drug targets and the signature in the interactome, we identified several potential candidates and provided a drug list ranked by their proximity. Taken together, we revealed a novel gene expression signature as a theragnostic biomarker for PCPF by integrating different computational approaches. Moreover, we showed that network-based proximity could be used as a framework to repurpose drugs for PCPF.
Citric Acid: A Multifunctional Pharmaceutical Excipient
Authors: Lambros Maria , Tran Thac (Henry) , Fei Qinqin , Nicolaou Mike
Published: May 2022

Citric acid, a tricarboxylic acid, has found wide application in the chemical and pharmaceutical industry due to its biocompatibility, versatility, and green, environmentally friendly chemistry. This review emphasizes the pharmaceutical uses of citric acid as a strategic ingredient in drug formulation while focusing on the impact of its physicochemical properties. The functionality of citric acid is due to its three carboxylic groups and one hydroxyl group. These allow it to be used in many ways, including its ability to be used as a crosslinker to form biodegradable polymers and as a co-former in co-amorphous and co-crystal applications. This paper also analyzes the effect of citric acid in physiological processes and how this effect can be used to enhance the attributes of pharmaceutical preparations, as well as providing a critical discussion on the issues that may arise out of the presence of citric acid in formulations.
Ionizable Lipid Nanoparticles Enhanced the Synergistic Adjuvant Effect of CpG ODNs and QS21 in a Varicella Zoster Virus Glycoprotein E Subunit Vaccine
Authors: Luan Ning , Cao Han , Wang Yunfei , Lin Kangyang , Liu Cunbao
Published: May 2022

Varicella zoster virus (VZV) causes two diseases: varicella upon primary infection and herpes zoster when latent viruses in the sensory ganglia reactivate. While varicella vaccines depend on humoral immunity to prevent VZV infection, cell-mediated immunity (CMI), which plays a therapeutic role in the control or elimination of reactivated VZV in infected cells, is decisive for zoster vaccine efficacy. As one of the most abundant glycoproteins of VZV, conserved glycoprotein E (gE) is essential for viral replication and transmission between ganglion cells, thus making it an ideal target subunit vaccine antigen; gE has been successfully used in the herpes zoster vaccine ShingrixTM on the market. In this report, we found that ionizable lipid nanoparticles (LNPs) approved by the Food and Drug Administration (FDA) as vectors for coronavirus disease 2019 (COVID-19) mRNA vaccines could enhance the synergistic adjuvant effect of CpG oligodeoxynucleotides (CpG ODNs) and QS21 on VZV-gE, affecting both humoral immunity and CMI. Vaccines made with these LNPs showed promise as varicella vaccines without a potential risk of herpes zoster, which identifies them as a novel type of herpes zoster vaccine similar to ShingrixTM. All of the components in this LNP-CpG-QS21 adjuvant system were proven to be safe after mass vaccination, and the high proportion of cholesterol contained in the LNPs was helpful for limiting the cytotoxicity induced by QS21, which may lead to the development of a novel herpes zoster subunit vaccine for clinical application.
Impaired Pharmacokinetics of Amiodarone under Veno-Venous Extracorporeal Membrane Oxygenation: From Bench to Bedside
Authors: Lescroart Mickaël , Pressiat Claire , Péquignot Benjamin , Tran N’Guyen , Hébert Jean-Louis , Alsagheer Nassib , Gambier Nicolas , Ghaleh Bijan , Scala-Bertola Julien , Levy Bruno
Published: May 2022

Background: Adjusting drug therapy under veno-venous extracorporeal membrane oxygenation (VV ECMO) is challenging. Although impaired pharmacokinetics (PK) under VV ECMO have been reported for sedative drugs and antibiotics, data about amiodarone are lacking. We evaluated the pharmacokinetics of amiodarone under VV ECMO both in vitro and in vivo. Methods: In vitro: Amiodarone concentration decays were compared between closed-loop ECMO and control stirring containers over a 24 h period. In vivo: Potassium-induced cardiac arrest in 10 pigs with ARDS, assigned to either control or VV ECMO groups, was treated with 300 mg amiodarone injection under continuous cardiopulmonary resuscitation. Pharmacokinetic parameters Cmax, Tmax AUC and F were determined from both direct amiodarone plasma concentrations observation and non-linear mixed effects modeling estimation. Results: An in vitro study revealed a rapid and significant decrease in amiodarone concentrations in the closed-loop ECMO circuitry whereas it remained stable in control experiment. In vivo study revealed a 32% decrease in the AUC and a significant 42% drop of Cmax in the VV ECMO group as compared to controls. No difference in Tmax was observed. VV ECMO significantly modified both central distribution volume and amiodarone clearance. Monte Carlo simulations predicted that a 600 mg bolus of amiodarone under VV ECMO would achieve the amiodarone bioavailability observed in the control group. Conclusions: This is the first study to report decreased amiodarone bioavailability under VV ECMO. Higher doses of amiodarone should be considered for effective amiodarone exposure under VV ECMO.
A Multi-Layer Erbium-Doped Air-Hole-Assisted Few-Mode Fiber with Ultra-Low Differential Modal Gain
Authors: Li Zhiqi , Pei Li , Zheng Jingjing , Wang Jianshuai , Xu Wenxuan , Ning Tigang , Li Jing
Published: May 2022

The air-hole assisted few-mode fiber (AH-FMF) enables modal intensity balance and offers a profound prospect in gain equalization with the combination of adaptive ion doping. In this paper, we proposed an AH-FM-EDF with a multi-layered erbium doping profile. In AH-FM-EDF, due to the central air hole, only the first radial order modes (LP01, LP11, LP21, and LP31) are supported, and all the modes are confined in the same high refractive index core region. The differential modal gain (DMG) is highly reduced by optimizing the erbium doping proportion in each layer. Compared with uniform doping, the DMG is reduced from 4 dB to 0.14 dB as triple-layer doping is deployed. Additionally, the proposed erbium-doped fiber performs well in gain flattening and fabrication tolerance over the whole C-band.
Study on Supergenus Rubus L.: Edible, Medicinal, and Phylogenetic Characterization
Authors: Meng Qinglin , Manghwar Hakim , Hu Weiming
Published: May 2022

Rubus L. is one of the most diverse genera belonging to Rosaceae; it consists of more than 700 species with a worldwide distribution. It thus provides an ideal natural “supergenus” for studying the importance of its edible, medicinal, and phylogenetic characteristics for application in our daily lives and fundamental scientific studies. The Rubus genus includes many economically important species, such as blackberry (R. fruticosus L.), red raspberry (R. ideaus L.), black raspberry (R. occidentalis L.), and raspberry (R. chingii Hu), which are widely utilized in the fresh fruit market and the medicinal industry. Although Rubus species have existed in human civilization for hundreds of years, their utilization as fruit and in medicine is still largely inadequate, and many questions on their complex phylogenetic relationships need to be answered. In this review, we briefly summarize the history and progress of studies on Rubus, including its domestication as a source of fresh fruit, its medicinal uses in pharmacology, and its systematic position in the phylogenetic tree. Recent available evidence indicates that (1) thousands of Rubus cultivars were bred via time- and labor-consuming methods from only a few wild species, and new breeding strategies and germplasms were thus limited; (2) many kinds of species in Rubus have been used as medicinal herbs, though only a few species (R. ideaus L., R. chingii Hu, and R. occidentalis L.) have been well studied; (3) the phylogeny of Rubus is very complex, with the main reason for this possibly being the existence of multiple reproductive strategies (apomixis, hybridization, and polyploidization). Our review addresses the utilization of Rubus, summarizing major relevant achievements and proposing core prospects for future application, and thus could serve as a useful roadmap for future elite cultivar breeding and scientific studies.
Environmental Behaviors of Bacillus thuringiensis (Bt) Insecticidal Proteins and Their Effects on Microbial Ecology
Authors: Li Yujie , Wang Cui , Ge Lei , Hu Cong , Wu Guogan , Sun Yu , Song Lili , Wu Xiao , Pan Aihu , Xu Qinqing , Shi Jialiang , Liang Jingang , Li Peng
Published: May 2022

Bt proteins are crystal proteins produced by Bacillus thuringiensis (Bt) in the early stage of spore formation that exhibit highly specific insecticidal activities. The application of Bt proteins primarily includes Bt transgenic plants and Bt biopesticides. Transgenic crops with insect resistance (via Bt)/herbicide tolerance comprise the largest global area of agricultural planting. After artificial modification, Bt insecticidal proteins expressed from Bt can be released into soils through root exudates, pollen, and plant residues. In addition, the construction of Bt recombinant engineered strains through genetic engineering has become a major focus of Bt biopesticides, and the expressed Bt proteins will also remain in soil environments. Bt proteins expressed and released by Bt transgenic plants and Bt recombinant strains are structurally and functionally quite different from Bt prototoxins naturally expressed by B. thuringiensis in soils. The former can thus be regarded as an environmentally exogenous substance with insecticidal toxicity that may have potential ecological risks. Consequently, biosafety evaluations must be conducted before field tests and production of Bt plants or recombinant strains. This review summarizes the adsorption, retention, and degradation behavior of Bt insecticidal proteins in soils, in addition to their impacts on soil physical and chemical properties along with soil microbial diversity. The review provides a scientific framework for evaluating the environmental biosafety of Bt transgenic plants, Bt transgenic microorganisms, and their expression products. In addition, prospective research targets, research methods, and evaluation methods are highlighted based on current research of Bt proteins.
The Impact of Germinated Chickpea Flour Addition on Dough Rheology and Bread Quality
Authors: Atudorei Denisa , Atudorei Olivia , Codină Georgiana Gabriela
Published: May 2022

The research focused on the effect of germinated chickpea flour (GCF) in a lyophilized form on dough rheology, microstructure and bread quality. The GCF addition levels in refined wheat flour with a low α-amylase activity were 5%, 10%, 15% and 20%, up to an optimum falling number value of the mixed flour. Generally, the dough rheological properties of water absorption, tolerance to mixing, dough consistency, dough extensibility, index of swelling, baking strength and loss tangent (tan δ) for the temperature sweep test decreased with the increased level of GCF addition, whereas the total volume of gas production and G′ and G″ modules for the temperature sweep test increased. Dough microstructure analyzed by epifluorescence light microscopy (EFLM) clearly showed a change in the starch and gluten distribution from the dough system by an increase in protein and a decrease in starch granules phase with the increased level of GCF addition in wheat flour. The bread physical characteristics (loaf volume, porosity, elasticity) and sensory ones were improved with up to 15% GCF addition in wheat flour. The bread firmness increased, whereas the bread gumminess, cohesiveness and resilience decreased with increased GCF addition in wheat flour. The bread crust and crumb color of the bread samples become darker with an increased GCF addition in the bread recipe.
Active and Passive Sustainability: Measuring the Anti-Fragility of Territories
Authors: Notarstefano Giuseppe
Published: May 2022

Sustainability is a paradigm of generative action if combined with the principle of antifragility. This contribution, adhering to a transdisciplinary approach, proposes a reinterpretation of the principles of the 2030 Agenda, orienting them towards a community-building model. Sustainability can be seen as passive (care) and active (custody), determining paths to be activated at the territorial level. Sustainability is a communitarian learning path measured by one’s antifragility capacity. The effectiveness of the Agenda is linked to the dissemination and accountability of the challenges contained in the 17 objectives. The local scale is perhaps the most suitable for activating this process. However, this requires a strengthening of the anti-fragility of communities and organizations, which must be more capable of recognizing and reducing vulnerabilities and weaknesses. A promising field of application is that of impact assessment, to be reworked in the light of the antifragility approach.
Eu3+- and Tb3+-Based Coordination Complexes of Poly(N-Isopropyl,N-methylacrylamide-stat-N,N-dimethylacrylamide) Copolymer: Synthesis, Characterization and Property
Authors: Li Jian , Cui Guihua , Bi Siyuan , Cui Xu , Li Yanhui , Duan Qian , Kakuchi Toyoji , Chen Yougen
Published: May 2022

This contribution reports the syntheses, structural analyses and properties of europium (Eu3+)- and terbium (Tb3+)-based coordination complexes of poly(N-isopropyl,N-methylacrylamide-stat-N,N-dimethylacrylamide) (poly(iPMAm-stat-DMAm)) copolymer, named as poly-Eu(III) and poly-Tb(III), respectively. In greater detail, poly(iPMAm85-stat-DMAm15) is first prepared by random copolymerization of N-isopropyl,N-methylacrylamide (iPMAm) and N,N-dimethylacrylamide (DMAm) via group transfer polymerization (GTP). Next, poly(iPMAm85-stat-DMAm15) is used as the polymer matrix for chelating with Eu3+ and Tb3+ cations at its side amide groups, to produce poly-Eu(III) and poly-Tb(III). Their structural characterizations by FT-IR spectroscopy and XPS confirm the formation of polymeric complexes. The study on their fluorescence emission characteristics and luminescence lifetime demonstrates that Poly-Eu(III) shows four strong emission peaks at 578, 593, 622, and 651 nm, which are responsible for the electron transitions from the excited 5D0 state to the multiplet 7FJ (J = 0, 1, 2, 3) states, respectively, and poly-Tb(III) also displays four emission peaks at 489, 545, 588, and 654 nm, mainly due to the electron transitions of 5D4 → 7Fi (i = 6, 5, 4, 3). The luminescence lifetimes of poly-Eu(III) (τpoly-Eu(III)) and poly-Tb(III) (τpoly-Tb(III)) are determined to be 4.57 and 7.50 ms, respectively. In addition, in aqueous solutions, poly-Eu(III) and poly-Tb(III) are found to exhibit thermoresponsivity, with their cloud temperatures (Tcs) locating around 36.4 and 36.8 °C, respectively. Finally, the cytotoxicity study on the human colon carcinoma cells LoVo and DLD1 suggests that the luminescent Eu3+ and Tb3+ in the chelated state with poly(iPMAm-stat-DMAm) show much better biocompatibility and lower toxicity than their inorganic salts.
Fluorescence Labeling of Cellulose Nanocrystals—A Facile and Green Synthesis Route
Authors: Campora Lorenzo Donato , Metzger Christoph , Dähnhardt-Pfeiffer Stephan , Drexel Roland , Meier Florian , Fürtauer Siegfried
Published: May 2022

Efficient chemical modification of cellulose nanocrystals (CNCs) by grafting commonly involves aprotic solvents, toxic reactants, harsh reaction conditions, or catalysts, which have negative effects on the particle character, reduced dispersibility and requires further purification, if products are intended for biomedical applications. This work, in contrast, presents a robust, facile, and green synthesis protocol for the grafting of an amino-reactive fluorophore like fluorescein isothiocyanate (FITC) on aqueous CNCs, combining and modifying existent approaches in a two-step procedure. Comparably high grafting yields were achieved, which were confirmed by thermogravimetry, FTIR, and photometry. The dispersive properties were confirmed by DLS, AF4-MALS, and TEM studies. The presented route is highly suitable for the introduction of silane-bound organic groups and offers a versatile platform for further modification routes of cellulose-based substrates.
A Machine Learning Model for Torsion Strength of Externally Bonded FRP-Reinforced Concrete Beams
Authors: Deifalla Ahmed , Salem Nermin M.
Published: May 2022

Strengthening of reinforced concrete (RC) beams subjected to significant torsion is an ongoing area of research. In addition, fiber-reinforced polymer (FRP) is the most popular choice as a strengthening material due to its superior properties. Moreover, machine learning models have successfully modeled complex behavior affected by many parameters. This study will introduce a machine learning model for calculating the ultimate torsion strength of concrete beams strengthened using externally bonded (EB) FRP. An experimental dataset from published literature was collected. Available models were outlined. Several machine learning models were developed and evaluated. The best model was the wide neural network, which had the most accurate results with a coefficient of determination, root mean square error, mean average error, an average safety factor, and coefficient of variation values of 0.93, 1.66, 0.98, 1.11, and 45%. It was selected and further compared with the models from the existing literature. The model showed an improved agreement and consistency with the experimental results compared to the available models from the literature. In addition, the effect of each parameter on the strength was identified and discussed. The most dominant input parameter is effective depth, followed by FRP-reinforcement ratio and strengthening scheme, while fiber orientation has proven to have the least effect on the prediction output accuracy.
Phyto Synthesis of Manganese-Doped Zinc Nanoparticles Using Carica papaya Leaves: Structural Properties and Its Evaluation for Catalytic, Antibacterial and Antioxidant Activities
Authors: Alam Mir Waqas , Al Qahtani Hassan S. , Aamir Muhammad , Abuzir Alaaedeen , Khan Muhammad Shuaib , Albuhulayqah Maryam , Mushtaq Shehla , Zaidi Noushi , Ramya Ambikapathi
Published: May 2022

The current study aims to synthesize bimetal oxide nanoparticles (zinc and manganese ions) using the carica papaya leaf extract. The crystallite size of the nanoparticle from X-ray diffraction method was found to be 19.23 nm. The nanosheet morphology was established from Scanning Electron Microscopy. Energy-dispersive X-ray diffraction was used to determine the elemental content of the synthesized material. The atomic percentage of Mn and Zn was found to be 15.13 and 26.63. The weight percentage of Mn and Zn was found to be 7.08 and 10.40. From dynamic light scattering analysis, the hydrodynamic diameter and zeta potential was found to be 135.1 nm and −33.36 eV. The 1,1-diphenyl-2-picryl hydroxyl radical, hydroxyl radical, FRAP, and hydrogen peroxide scavenging tests were used to investigate the antioxidant activity of Mn-Zn NPs. Mn-Zn NPs have substantial antioxidant properties. The photocatalytic activity of the Mn-Zn NPs was assessed by their ability to degrade Erichrome black T (87.67%), methyl red dye (78.54%), and methyl orange dye (69.79%). Additionally, it had significant antimicrobial action S. typhi showed a higher zone of inhibition 14.3 ± 0.64 mm. Mn-Zn nanoparticles were utilized as a catalyst for p-nitrophenol reduction. The bimetal oxide Mn-Zn NPs synthesized using C. papaya leaf extract exhibited promising dye degradation activity in wastewater treatment. Thus, the aforementioned approach will be a novel, low cost and ecofriendly approach.
Synthesis and Characterization of Metallopolymer Networks Featuring Triple Shape-Memory Ability Based on Different Reversible Metal Complexes
Authors: Meurer Josefine , Bätz Thomas , Hniopek Julian , Jäger Milena , Zechel Stefan , Schmitt Michael , Popp Jürgen , Hager Martin D. , Schubert Ulrich S.
Published: May 2022

This study presents the synthesis and characterization of metallopolymer networks with a triple shape-memory ability. A covalently crosslinked polymer network featuring two different additional ligands in its side chains is synthesized via free radical polymerization (FRP). The subsequent addition of different metal salts leads to the selective formation of complexes with two different association constants (Ka), proven via isothermal titration calorimetry (ITC). Those two supramolecular crosslinks feature different activation temperatures and can act as two individual switching units enabling the fixation and recovery of two temporary shapes. The presented samples were investigated in a detailed fashion via differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and FT-Raman spectroscopy. Furthermore, thermo-mechanical analyses (TMA) revealed excellent dual and triple shape-memory abilities of the presented metallopolymer networks.
Influence of Rapid Consolidation on Co-Extruded Additively Manufactured Composites
Authors: Savandaiah Chethan , Sieberer Stefan , Plank Bernhard , Maurer Julia , Steinbichler Georg , Sapkota Janak
Published: May 2022

Composite filament co-extrusion (CFC) additive manufacturing (AM) is a bi-matrix rapid fabrication technique that is used to produce highly customisable composite parts. By this method, pre-cured, thermoset-based composite carbon fibre (CCF) is simultaneously extruded along with thermoplastic (TP) binding melt as the matrix. Like additive manufacturing, CFC technology also has inherent challenges which include voids, defects and a reduction in CCF’s volume in the fabricated parts. Nevertheless, CFC AM is an emerging composite processing technology, a highly customisable and user-oriented manufacturing unit. A new TP-based composites processing technique has the potential to be synergised with conventional processing techniques such as injection moulding to produce lightweight composite parts. Thus, CFC AM can be a credible technology to replace unsustainable subtractive manufacturing, if only the defects are minimised and processing reliability is achieved. The main objective of this research is to investigate and reduce internal voids and defects by utilising compression pressing as a rapid consolidation post-processing technique. Post-processing techniques are known to reduce the internal voids in AM-manufactured parts, depending on the TP matrices. Accordingly, the rapid consolidated neat polylactic acid (PLA) TP matrix showed the highest reduction in internal voids, approximately 92%. The PLA and polyamide 6 (PA6) binding matrix were reinforced with short carbon fibre (SCF) and long carbon fibre (LCF), respectively, to compensate for the CCF’s fibre volume reduction. An increase in tensile strength (ca. 12%) and modulus (ca. 30%) was observed in SCF-filled PLA. Furthermore, an approximately 53% increase in tensile strength and a 76% increase in modulus for LCF-reinforced PA6 as the binding matrix was observed. Similar trends were observed in CFC and rapidly consolidated CFC specimens’ flexural properties, resulting due to reduced internal voids.
Direct Visualization of UV-Light on Polymer Composite Films Consisting of Light Emitting Organic Micro Rods and Polydimethylsiloxane
Authors: Kim Misuk , Kim Jiyoun , Kim Hyeonwoo , Jung Incheol , Kwak Hojae , Lee Gil Sun , Na Young Jun , Hong Young Ki , Park Dong Hyuk , Lee Kyu-Tae
Published: May 2022

We experimentally demonstrate the direct visualization of ultraviolet (UV) light using flexible polymer composite films consisting of crystalline organic tris-(8-hydroxyquinoline) aluminum (Alq3) micro-rods and polydimethylsiloxane (PDMS). The representative organic mono-molecule Alq3, which is a core material of organic light-emitting diodes, was used to detect light in the invisible UV region and visualize photoluminescence (PL). Alq3 shows absorption in the UV region and light-emitting characteristics in the green region, making it an optimal material for UV visualization because of its large Stokes transition. Crystalline Alq3 micro-rods were fabricated in a deionized water solution through a sequential process of reprecipitation and self-assembly. Highly bright photoluminescence was observed on the highly crystalline Alq3 micro-rods under UV light excitation, indicating that the crystalline structures of Alq3 molecules affect the visible emission decay of excitons. The Alq3 micro-rods were manufactured as flexible polymer composite films using a PDMS solution to observe UV photodetector characteristics according to UV intensity, and it was confirmed that the intensity of the fine UV light reaching the earth’s surface can be visualized by making use of this UV photodetector.
Influence of Resin Content and Density on Water Resistance of Bamboo Scrimber Composite from a Bonding Interface Structure Perspective
Authors: Ji Yaohui , Lei Wencheng , Huang Yuxiang , Wu Jiangyuan , Yu Wenji
Published: May 2022

As a new type of green environmental protection material for outdoor use, the water resistance of bamboo scrimber composite (BSC) is crucial—the primary reason for a decrease in water resistance being bonding interface failure. From a bonding interface structure perspective, the influence mechanism of the resin content and density on the water resistance of BSCs remains unknown. Therefore, in this study, BSCs were prepared using Moso bamboo and phenol-formaldehyde resin, and the changes in the macroscopic and microscopic bonding interfaces before and after 28-h water-resistance tests were observed and analyzed. The results showed that the water resistance of the BSC increased with increasing resin content, with higher thickness swelling rates (TSRs) observed at higher densities. Obvious cracks were found at the macroscopic interface after 28-h tests, with higher resin contents leading to fewer and smaller cracks. With increasing density, the longitudinal fissures due to defibering process decreased, having an effect on width swelling rates (WSRs). Furthermore, porosity measurements revealed changes in the microscopic bonding interface; the difference in porosity before and after testing (D-value) showed the same trend as water resistance. Generally, we conclude that the macroscopic and microscopic bonding interface structures are closely related to BSC water resistance.
Experimental and Numerical Investigation on Fatigue Properties of Carbon Fiber Cross-Ply Laminates in Hygrothermal Environments
Authors: Xu Mingrui , Zeng Benyin , An Ziqian , Xiong Xin , Cheng Xiaoquan
Published: May 2022

The fatigue properties of composite materials are degraded seriously in hygrothermal environments, so taking into account their influence is very important when evaluating the fatigue life of composite structures. Tensile fatigue experiments of carbon fiber reinforced resin composite cross-ply laminates were conducted in room temperature/dry (RTD), cool temperature/dry (CTD) and elevated temperature/wet (ETW) conditions. The S-N curves and fatigue failure modes of the cross-ply laminates were obtained in three conditions. On this basis, a finite element model was established to discuss the influence of temperature and moisture content on the fatigue properties, as well as a method for determining environmental factors of fatigue life of cross-ply laminates was established. The results show that the saturation moisture absorption and temperature have a significant influence on the tensile fatigue properties of cross-ply laminates. The high-cycle fatigue property is weakened significantly by the saturation moisture absorption and high temperature, but the low-cycle fatigue properties were strengthened in cool temperature conditions. The delamination failure mode in ETW is the most severe, presenting with an obvious necking phenomenon. The influence of temperature has a greater effect than that of moisture content, but moisture absorption would play its affect obviously when temperature exceeds 40 °C.
Dynamic and Ballistic Performance of Graphene Oxide Functionalized Curaua Fiber-Reinforced Epoxy Nanocomposites
Authors: Costa Ulisses Oliveira , Nascimento Lucio Fabio Cassiano , Bezerra Wendell Bruno Almeida , Neves Pamela Pinto , Huaman Noemi Raquel Checca , Monteiro Sergio Neves , Pinheiro Wagner Anacleto
Published: May 2022

Graphene oxide (GO) functionalized curaua fiber (CF) has been shown to improve the mechanical properties and ballistic performance of epoxy matrix (EM) nanocomposites with 30 vol% fiber. However, the possibility of further improvement in the property and performance of nanocomposites with a greater percentage of GO functionalized CF is still a challenging endeavor. In the present work, a novel epoxy composite reinforced with 40 vol% CF coated with 0.1 wt% GO (40GOCF/EM), was subjected to Izod and ballistic impact tests as well as corresponding fractographic analysis in comparison with a GO-free composite (40CF/EM). One important achievement of this work was to determine the characteristics of the GO by means of FE-SEM and TEM. A zeta potential of −21.46 mV disclosed a relatively low stability of the applied GO, which was attributed to more multilayered structures rather than mono- or few-layer flakes. FE-SEM images revealed GO deposition, with thickness around 30 nm, onto the CF. Izod impact-absorbed energy of 813 J/m for the 40GOCF/EM was not only higher than that of 620 J/m for the 40CF/EM but also higher than other values reported for fiber composites in the literature. The GO-functionalized nanocomposite was more optimized for ballistic application against a 7.62 mm projectile, with a lower depth of penetration (24.80 mm) as compared with the 30 vol% GO-functionalized CF/epoxy nanocomposite previously reported (27.43 mm). Fractographic analysis identified five main events in the ballistic-tested 40GOCF/EM composed of multilayered armor: CF rupture, epoxy matrix rupture, CF/matrix delamination, CF fibril split, and capture of ceramic fragments by the CF. Microcracks were associated with the morphological aspects of the CF surface. A brief cost-effective analysis confirmed that 40GOCF/EM may be one of the most promising materials for personal multilayered ballistic armor.
Near-Infrared Light-Remote Localized Drug Delivery Systems Based on Zwitterionic Polymer Nanofibers for Combination Therapy
Authors: Li Yu-Lun , Chen Ching-Yi
Published: May 2022

Localized drug delivery systems (LDDS) have gained great interests because they can directly treat the tumors and minimize systematic toxicity, and maximize drug action by controlling release precisely at the tumor site. However, the resistance of the non-specific adsorption of biomolecules is also important to alleviate the inflammatory reactions and avoid the decrease in performance of LDDS. In this study, we develop a near infrared (NIR) light-triggered nanofibrous delivery system consisting of zwitterionic poly(2-methacryloyloxyethyl phosphorylcholine)-b-poly(ε-caprolactone) (PMPC-b-PCL) encapsulated with indocyanine green (ICG) and doxorubicin (DOX) for dual photothermal therapy and chemotherapy. The nanofibrous mat shows hydrophilic characteristics and good antifouling performance. Under mild NIR irradiation, ICG could convert NIR light into thermal energy that elevates the surrounding temperature above 45 °C. This thermal energy also markedly accelerates the DOX release from the nanofibrous mat due to softening of the nanofibers, indicating the drug release could be controlled and switched on/off by light-triggering. Moreover, this light-triggered thermal energy and releasing behavior contribute to enhancing the cell lethality. Intracellular DOX distribution confirms the more drugs release upon light irradiation. All results demonstrate the developed light-triggered drug release nanofibers as LDDS are biocompatible and antifouling as well as has the superior combinational chemotherapy/photothermal therapy.
From Waste to Schiff Base: Upcycling of Aminolysed Poly(ethylene terephthalate) Product
Authors: Otaibi Ahmed A. Al , Alsukaibi Abdulmohsen Khalaf Dhahi , Rahman Md. Ataur , Mushtaque Md. , Haque Ashanul
Published: May 2022

Recycling plastic waste into valuable materials is one of the contemporary challenges. Every year around 50 million tons of polyethylene terephthalate (PET) bottles are used worldwide. The fact that only a part of this amount is being recycled is putting a burden on the environment. Therefore, a technology that can convert PET-based waste materials into useful ones is highly needed. In the present work, attempts have been made to convert PET-based waste materials into a precursor for others. We report an aminolysed product (3) obtained by aminolysis reaction of PET (1) with 1,2 diaminopropane (DAP, 2) under solvent and catalytic free conditions. The highest amount of monomeric product was obtained upon heating the mixture of diamine and PET at 130 °C. The resulting aminolysed product was then converted to a Schiff-base (5) in 25% yield. The chemical structure of the synthesized compounds was confirmed using multi-spectroscopic techniques. The results of this study will be a valuable addition to the growing body of work on plastic recycling.