bluuebunny/crossref_metadata_2025 huggingface dataset:
Determination of Temperature of Components of Cotton-Raw Material in a Drum Dryer with a Constant
Authors: Mamatov A Z , Usmankulov A K , Abbazov I Z , Norboyev U A , Mukhametshina E T
Published: December 2021

This article solves one parabolic-type boundary value problem for determining the heat-moisture state of raw cotton in drum dryers at a constant air temperature. Numerical results are obtained by the Bubnov – Galerkin method of the problem under consideration, a comparative analysis is carried out with experimental data. It is shown that the proposed mathematical model and its numerical algorithm adequately describe the drying process of raw cotton.
Construction of mathematical modelling of a population of microalgae
Authors: Rakhmanov Sh , Abdullaeva D , Azizova N , Nigmatov A
Published: December 2021

This article devoted to the development of the mathematic model of the technological process of the chlorella cultivation process, its features and solving of this mathematic model. The Exponential growth of microalgae population under conditions of unlimited nutrient resources and population space proceeds at a rate proportional to the number of species of predominant cells and is described by the differential equation. In the presence of several inhibitors, specific velocity equations with the number of inhibitors can be used, but, as a rule, there are practically no elements acting as inhibitors in the cultivation of Chlorella microalgae. The modeling of this particular class of objects did not take into account the effect of inhibitors on the growth of microalgae. The consumption of nutrients to support the life of microalgae is described by the differential equation. In the course of this work, the processes of cultivation of microalgae were brought together into a system of equations. As a result, a system of differential equations of the technological process of Chlorella cultivation was obtained. Thus, the obtained system of equations describes the process of cultivation of microalgae and its technological process, implemented in a periodic mode.
Study on some features of water exchange of local varieties of hard wheat
Authors: Khodzhaeva N D , Urazbayev I U , Ishankulova K K
Published: December 2021

This article discusses the main physiological indicators of water exchange in winter wheat varieties Makus-3, Istiklal and Istiklal-20. There were revealed indicators of daily and residual water deficit of leaves, as well as the water potential of leaves.As you are aware, drought resistance of plants depends on a number of factors. In particular, it is largely determined by the biological characteristics of plants.In this regard, the identification of the features of the physiological processes of the water regime and the associated metabolic processes that determine the resistance to drought in various varieties and hybrids of wheat is of great interest not only in scientific but also in practical terms, because the research results can be used to develop recommendations for selection of source forms for breeding work. We have studied a number of indicators of the water regime of local varieties of winter wheat -Istiklal, Istiklal-20 and Makus-3. The research was carried out on plants grown under the conditions of vegetation and field experiments in the phases of booting, earing, flowering and waxy ripeness.
Characterization of expansive soils for the foundation of an irrigation canal in the Peruvian Andes, Cabana-Mañazo case
Authors: Pilares-Hualpa I A , Alfaro-Alejo R , Pilares-Calla C A , Alfaro-Vilca O E
Published: December 2021

Alterations in water content in swelling soils cause volume variation, which implies constructive, socioeconomic and environmental damage. This paper characterizes the swelling soil located in an irrigation canal of the Peruvian Altiplano and its behavior of the properties by addition of lime in 5, 10, 15 and 20% of the total weight. Finding that the sample of the station 6+575 has combined presence of montmorillonite clays in a percentage of 13.52% together with the group of kaolinites in a percentage of 1.31%, consequently, it makes expandable clay of high plasticity. The soils of the station 6+250 have the characteristics of kaolinite clay, which distinguishes it as having low plasticity. In the swelling tests the high expansiveness was found, in the station 6+575, which has decreased with the addition of lime. Considering that concrete canals are generally of small thickness it would be important to consider the slightly dangerous effects on irrigation infrastructure.
Study on combined machine for the subsurface soil treatment
Authors: Ravshanov Kh , Mamatov F , Mukimov B , Sultonov R , Abdullayev A , Murtazaeva G
Published: December 2021

The purpose of the study is to substantiate the relative position of the working bodies of a combined machine for non-fall tillage of winter crops. The design scheme and the principle of operation of the combined machine are given. The basic principles and methods of classical mechanics, mathematical analysis and statistics were used in this study. It is established that the most optimal design scheme of a combined machine for non-tillage of the soil and its preparation for sowing of repeated crops is considered to be a scheme consisting of non-tillage cases with crushers, a battery with cut-out spherical disks and a roller. According to the results of theoretical and experimental studies, it was found that with a longitudinal distance between the shaft-free bodies of 40 cm and a transverse distance of 90 cm, a longitudinal distance from the toe of the ploughshare to the center of the support wheel of 50 cm and a longitudinal distance from the toe of the ploughshare of the shaft-free body to the axis of rotation of the cut-out spherical disk of 120 cm, a longitudinal distance from the axis of rotation of the cut-out spherical disk to the center of the roller of 75 cm, high-quality tillage with minimal energy costs is provided.
Study on plowing of cotton soil using two-tier plow
Authors: Mamatov F , Temirov I , Berdimuratov P , Mambetsheripova A , Ochilov S
Published: December 2021

The purpose of the study is to substantiate the parameters of a two-tier plow for plowing soil from under cotton. The basic principles and methods of classical mechanics, mathematical analysis and statistics were used in this study. The effects of the cotton field relief on the tillage and traction resistance of a two-tier plow were studied theoretically and experimentally. Analytical expressions are obtained for determining the uniformity of the course, the load of the bodies and the center of resistance of the plow, depending on its main parameters and the roughness of the relief of the cotton field. It is established that serial two-tier plows, due to the discrepancy between their width of the gripper and the width of the row spacing, do not meet the requirements of agricultural technology: the plowing depth is not stable, the coefficient of variation of the plowing depth reaches 16% for a trailed plow, and for a mounted plow - 25.8%; the transverse direction of the plough the bottom of the furrow turns out to be stepped; the value of the traction resistance changes at each pass of the plow. To improve the quality of plowing fields from under cotton, a new plowing method has been developed, carried out by a two-tier plow, the width of which is a multiple of the width of the row spacing of cotton. The width of the plow bodies is equal to half the width of the row spacing.
Efficacy of Cicer arietinum L. & Vigna mungo L. in remediation of Hexavalent Chromium
Authors: Bansal R , Gauba P
Published: December 2021

Hexavalent Chromium is a major soil pollutant; thus, its remediation from soil deserves due attention. Phytoremediation is an area of active current research which is eco-friendly and economic. Use of leguminous plants for phytoremediation will improve soil quality, fertility and nutrient balance and would help in restoration of natural soil ecosystem. The present study focuses on the use of two commonly growing legumes; Cicer arietinum (RP1) and Vigna mungo (RP2) to explore their remediation potential towards Cr(VI) with concentration ranging from 100-900 mg kg−1 with the growth up to three weeks and were assessed for remediation potential and toxicity parameters. Higher percentage of decrease in root and shoot length was observed in RP2 as compared to RP1. Chlorophyll content was also found to be decreasing with increasing Cr stress in both the species. RP2 recorded higher BCF than RP1. Highest bioaccumulation factor 4.32 was observed in RP2 at 400 mg kg−1 concentration. Translocation factor >1 was observed in both the plants with highest as 1.67 at 600 mg kg−1 in RP2 and 1.93 at 400 mg kg−1 in RP1. Remediation percentage of 72.25% in RP2 at 600 mg kg−1 and 73.13% at 400 mg kg−1 in RP1 was observed. Both the plants showed high tolerance and remediation potential towards Cr(VI) therefore has a great phytoremediation prospect, however, RP2 can be preferred over RP1.
Physicochemical properties of graft copolymers of collagen and fibroin with polyacrylic acid
Authors: Karimov S , Rafikov A , Nabiev N
Published: December 2021

Certain physicochemical properties of the synthesized graft copolymers of raw skin collagen and natural silk fibroin with polyacrylic acid have been determined. The dependence of the solubility, density, and thermal properties of copolymers on the ratio of components and synthesis conditions has been established.
Research of quality silk cocoon making technology
Authors: Gulamov A , Avazov K , Abrayqulov B
Published: December 2021

This article presents the period of wrapping a silkworm in a cocoon during the season of making cocoons, as well as the results of experiments on their collection and analysis, existing problems. Based on the results, proposals were made to improve the system of attracting the population to entrepreneurship and the development of entrepreneurship. In particular, the effectiveness of organizing capacities for the production of raw silk by cocooning a silkworm at home, serving the enterprises of the silk cluster, is explained.
Types and composition of diseases and pests of restructured forest and pasture plants in the dry part of the Aral Sea
Authors: Khujaev O , Obidjanov D , Tursunov O , Nazarova O
Published: December 2021

Studies on the dried bottom of the Aral Sea have shown that dominant disease from 3 species of them in Haloxylon plant is Podosphaera leucotricha and the dominant species from 34 species of pests is Big Dwarf Haloxylon Locust (D. albidula) and Small Haloxylon Locust (D. annulata roseipen). Chemical and biological control measures were taken against this disease and pests. When using anti-inflammatory drugs Redomil gold, Dnox, Fundazol and Sporagin, their effectiveness is as follows: Redomil gold - 68% sp (2.0–2.5 kg / ha), Fundazol -50% ke (2.0 l / ha), Dnox - 40% ke (2.0 l / ha) and Sporagin (4.0, l / ha) were tested using fungicides in the norms. Of the drugs used in the experimental variant, Redomil gold, 68% s.p. (2.0-2.5 kg / ha) was the highest biological efficacy against powdery mildew in the variant in which the fungicides were applied, was 91.3%.
Determination of model parameters of water-nutritional processes in soil for nitrogen compounds
Authors: Anarbaev A , Tursunov O , Kodirov D , Allenova I , Nazaraliev D , Obidjanov D
Published: December 2021

Studies of development of an integrated model that allows calculating the whole range of processes necessary for predicting and managing the water and nutrient regimes of reclaimed soils are considered. Calculated values of the maximum concentrations of nitrate nitrogen in the topsoil during the growing season of plant for Tashkent’s region are shown. Values of field moisture capacity and wilting moisture of the main varieties of irrigated soils are defined.
Study on the influence of rice paddies’ water layer temperature on rice yield
Authors: Rau A , Begmatov I , Rau G
Published: December 2021

Studies on Akdala rice system found that rice plants sensitive to meteorological conditions. Climate change leads to changes in crop structure. The temperature of the rice field air and water has a special influence on the structure of the rice crop. It is established that the temperature of the water layer in rice paddies in the irrigation period does not exceeds 290 C, which is below the threshold of 350 C, when the flow and discharge of water from the paddies are recommended. The temperature of water in rice paddies, the surface layer was determined by an express thermometer, at the depth of the water layer 5, 10, 15 and 20 cm by Savin Thermometers, soil – thermometers TM-5.
Choosing an appropriate planting scheme for intensive apple gardens
Authors: Boboeva H
Published: December 2021

This article describes the intensive planting of varieties of apples belonging to local and foreign selection in soil and climatic conditions of Tashkent region in 4 different (4.0x2.5; 4.0x2.0; 4.0x1.5 and 4.0x1.0 m.) planting schemes, mainly in varieties with low tree height and small branch size, the effect of planting schemes on tree habitat is small. In contrast, in varieties with high biological growth rate, the effect of planting schemes was found to be high. However, data have shown that as the length of the main branches in apple tree trees decreases, they cause an increase in ring-shaped fruit branches, which in turn affects the yield in the trees by reducing the feeding area.
Why illness is more important than disease in old age
Authors: Olde Rikkert Marcel G M , Melis René J F , Cohen Alan A , (Geeske) Peeters G M E E
Published: December 2021

Clinical reasoning and research in modern geriatrics often prioritises the disease concept. This is understandable as it has brought impressive advances in medicine (e.g. antibiotics, vaccines, successful cancer treatment and many effective surgeries). However, so far the disease framework has not succeeded in getting us to root causes of many age-related chronic diseases (e.g. Alzheimer’s disease, diabetes, osteoarthritis). Moreover, in aging and disease constructs alone fail to explain the variability in illness presentations.

Therefore, we propose to apply the underused illness concept in a new way by reconsidering the importance of common symptoms in the form of a dynamic network of symptoms as a complementary framework. We show that concepts and methods of complex system thinking now enable to fruitfully monitor and analyse the multiple interactions between symptoms in such in networks, offering new routes for prognosis and treatment. Moreover, close attention to the symptoms that bother older persons may also improve weighing the therapeutic objectives of well-being and survival and aligning treatment targets with the patients’ priorities.
HNetGO: protein function prediction via heterogeneous network transformer
Authors: Zhang Xiaoshuai , Guo Huannan , Zhang Fan , Wang Xuan , Wu Kaitao , Qiu Shizheng , Liu Bo , Wang Yadong , Hu Yang , Li Junyi
Published: December 2021

Protein function annotation is one of the most important research topics for revealing the essence of life at molecular level in the post-genome era. Current research shows that integrating multisource data can effectively improve the performance of protein function prediction models. However, the heavy reliance on complex feature engineering and model integration methods limits the development of existing methods. Besides, models based on deep learning only use labeled data in a certain dataset to extract sequence features, thus ignoring a large amount of existing unlabeled sequence data. Here, we propose an end-to-end protein function annotation model named HNetGO, which innovatively uses heterogeneous network to integrate protein sequence similarity and protein–protein interaction network information and combines the pretraining model to extract the semantic features of the protein sequence. In addition, we design an attention-based graph neural network model, which can effectively extract node-level features from heterogeneous networks and predict protein function by measuring the similarity between protein nodes and gene ontology term nodes. Comparative experiments on the human dataset show that HNetGO achieves state-of-the-art performance on cellular component and molecular function branches.
Contributions of obesity to kidney health and disease: insights from Mendelian randomization and the human kidney transcriptomics
Authors: Xu Xiaoguang , Eales James M , Jiang Xiao , Sanderson Eleanor , Drzal Maciej , Saluja Sushant , Scannali David , Williams Bryan , Morris Andrew P , Guzik Tomasz J , Charchar Fadi J , Holmes Michael V , Tomaszewski Maciej
Published: December 2021

Aims

Obesity and kidney diseases are common complex disorders with an increasing clinical and economic impact on healthcare around the globe. Our objective was to examine if modifiable anthropometric obesity indices show putatively causal association with kidney health and disease and highlight biological mechanisms of potential relevance to the association between obesity and the kidney.

Methods and results

We performed observational, one-sample, two-sample Mendelian randomization (MR) and multivariable MR studies in ∼300 000 participants of white-British ancestry from UK Biobank and participants of predominantly European ancestry from genome-wide association studies. The MR analyses revealed that increasing values of genetically predicted body mass index and waist circumference were causally associated with biochemical indices of renal function, kidney health index (a composite renal outcome derived from blood biochemistry, urine analysis, and International Classification of Disease-based kidney disease diagnoses), and both acute and chronic kidney diseases of different aetiologies including hypertensive renal disease and diabetic nephropathy. Approximately 13–16% and 21–26% of the potentially causal effect of obesity indices on kidney health were mediated by blood pressure and type 2 diabetes, respectively. A total of 61 pathways mapping primarily onto transcriptional/translational regulation, innate and adaptive immunity, and extracellular matrix and metabolism were associated with obesity measures in gene set enrichment analysis in up to 467 kidney transcriptomes.

Conclusions

Our data show that a putatively causal association of obesity with renal health is largely independent of blood pressure and type 2 diabetes and uncover the signatures of obesity on the transcriptome of human kidney.
Genome assembly, annotation, and comparative analysis of the cattail Typha latifolia
Authors: Widanagama Shane D , Freeland Joanna R , Xu Xinwei , Shafer Aaron B A
Published: December 2021

Cattails (Typha species) comprise a genus of emergent wetland plants with a global distribution. Typha latifolia and Typha angustifolia are two of the most widespread species, and in areas of sympatry can interbreed to produce the hybrid Typha × glauca. In some regions, the relatively high fitness of Typha × glauca allows it to outcompete and displace both parent species, while simultaneously reducing plant and invertebrate biodiversity, and modifying nutrient and water cycling. We generated a high-quality whole-genome assembly of T. latifolia using PacBio long-read and high coverage Illumina sequences that will facilitate evolutionary and ecological studies in this hybrid zone. Genome size was 287 Mb and consisted of 1158 scaffolds, with an N50 of 8.71 Mb; 43.84% of the genome were identified as repetitive elements. The assembly has a BUSCO score of 96.03%, and 27,432 genes and 2700 RNA sequences were putatively identified. Comparative analysis detected over 9000 shared orthologs with related taxa and phylogenomic analysis supporting T. latifolia as a divergent lineage within Poales. This high-quality scaffold-level reference genome will provide a useful resource for future population genomic analyses and improve our understanding of Typha hybrid dynamics.
Whole-Genome Sequencing ofAcer catalpifoliumReveals Evolutionary History of Endangered Species
Authors: Yu Tao , Hu Yiheng , Zhang Yuyang , Zhao Ran , Yan Xueqing , Dayananda Buddhi , Wang Jinpeng , Jiao Yuannian , Li Junqing , Yi Xin
Published: December 2021

Acer catalpifolium is an endangered species restricted to remote localities of West China. Understanding the genomic content and evolution of A. catalpifolium is essential to conservation efforts of this rare and ecologically valuable plant. Here, we report a high-quality genome of A. catalpifolium consisting of ∼654 Mbp and ∼35,132 protein-coding genes. We detected 969 positively selected genes in two Acer genomes compared with four other eudicots, 65 of which were transcription factors. We hypothesize that these positively selected mutations in transcription factors might affect their function and thus contribute to A. catalpifolium’s decline-type population. We also identified 179 significantly expanded gene families compared with 12 other eudicots, some of which are involved in stress responses, such as the FRS–FRF family. We inferred that A. catalpifolium has experienced gene family expansions to cope with environmental stress in its evolutionary history. Finally, 109 candidate genes encoding key enzymes in the lignin biosynthesis pathway were identified in A. catalpifolium; of particular note were the large range and high copy number of cinnamyl alcohol dehydrogenase genes. The chromosome-level genome of A. catalpifolium presented here may serve as a fundamental genomic resource for better understanding endangered Acer species, informing future conservation efforts.
Long-lived Humans Have a Unique Plasma Sphingolipidome
Authors: Pradas Irene , Jové Mariona , Huynh Kevin , Ingles Marta , Borras Consuelo , Mota-Martorell Natalia , Galo-Licona Jose Daniel , Puig Josep , Viña Jose , Meikle Peter J , Pamplona Reinald
Published: December 2021

A species-specific lipidome profile is an inherent feature linked to longevity in the animal kingdom. However, there is a lack of lipidomic studies on human longevity. Here, we use mass spectrometry-based lipidomics to detect and quantify 151 sphingolipid molecular species and use these to define a phenotype of healthy humans with exceptional life span. Our results demonstrate that this profile specifically comprises a higher content of complex glycosphingolipids (hexosylceramides and gangliosides), and lower levels of ceramide species from the de novo pathway, sphingomyelin and sulfatide; while for ceramide-derived signaling compounds, their content remains unchanged. Our findings suggest that structural glycosphingolipids may be more relevant to achieve the centenarian condition than signaling sphingolipids.
Holo-Transcriptome Sequences From the Tropical Marine SpongeCinachyrella alloclada
Authors: Desplat Yvain , Warner Jacob F , Lopez Jose V
Published: December 2021

Marine sponge transcriptomes are underrepresented in current databases. Furthermore, only 2 sponge genomes are available for comparative studies. Here we present the assembled and annotated holo-transcriptome of the common Florida reef sponge from the species Cinachyrella alloclada. After Illumina high-throughput sequencing, the data assembled using Trinity v2.5 confirmed a highly symbiotic organism, with the complexity of high microbial abundance sponges. This dataset is enriched in poly-A selected eukaryotic, rather than microbial transcripts. Overall, 39 813 transcripts with verified sponge sequence homology coded for 8496 unique proteins. The average sequence length was found to be 946 bp with an N50 sequence length of 1290 bp. Overall, the sponge assembly resulted in a GC content of 51.04%, which is within the range of GC bases in a eukaryotic transcriptome. BUSCO scored completeness analysis revealed a completeness of 60.3% and 60.1% based on the Eukaryota and Metazoa databases, respectively. Overall, this study points to an overarching goal of developing the C. alloclada sponge as a useful new experimental model organism.
IL-1 SRS Without the Bunker: Introduction and Clinical Experience of ZAP-X Gyroscopic Radiosurgery
Authors: Adler John R
Published: December 2021

Each year more than two million patients worldwide are potential candidates for SRS, yet due to the significant costs and complexities of historical delivery systems, only 150,000 patients currently receive such treatment. Japan Shonin-cleared in 2020, ZAP Surgical’s ZAP-X Gyroscopic Radiosurgery platform was designed to solve this challenge, and ultimately bring world-class SRS to more patients in more places.

ZAP-X is recognized for being the first and only vault-free SRS delivery system, thereby typically eliminating the need for providers to build costly shielded radiation treatment rooms. Utilizing a modern linear accelerator to produce radiation, ZAP-X is also the first and only dedicated radiosurgery system to no longer require Cobalt-60 radioactive sources, thereby eliminating the significant costs to license, secure and regularly replace live radioactive isotopes.

Built on a distinctive dual-gimbaled gantry design, the ZAP-X system uses gyroscopic mobility to direct radiosurgical beams from hundreds of unique angles to precisely concentrate radiation on the tumor target. This pioneering approach supports the clinical objective of protecting healthy brain tissue and patient neuro-cognitive function, as well as enable future potential SRS re-treatments without the unnecessary risks associated with multi-purpose radiation delivery technologies.
CBMS-10 Methionine metabolism closely related with self-renew, pluripotency and cell death in GICs through modification of cholesterol biosynthesis and ribosomal RNA
Authors: Yokogami Kiyotaka , Nakatake Yasutaka , Watanabe Takashi , Mizuguchi Asako , Yamashita Shinji , Takeshima Hideo
Published: December 2021

Glioma initiating cells (GICs) are the source of glioma cells that have the ability to self-renew and pluripotency, which are treatment-resistant, starting point for relapse and eventual death despite multimodality therapy. Since high accumulation is observed in 11cMet-PET at the time of recurrence, it is important to understand the mechanism of tumor cell activation caused by the reorganization of methionine metabolism. We cultured cells in methionine-deprived culture medium and performed a comprehensive analysis, and found that methionine depletion markedly decreased proliferation and increasing cell death of GICs. Decreased SAM, which is synthesized intracellularly catalyzed by methionine adenosyltransferase (MAT) using methionine, triggered the following: (i) global DNA demethylation, (ii) hyper-methylation of signaling pathways regulating pluripotentcy of stem cells, (iii) decreased expression of the core-genes and pluripotent marker of stem cells including FOXM1, SOX2, SOX4, PROM1 and OLIG2, (iv) decreased cholesterol synthesis and increased excretion mainly through decreased SREBF2 (v) down-regulation of the large subunit of ribosomal protein configured 28S and ACA43, snoRNA guiding the pseudouridylation of 28S ribosomal RNA, which has crucial role for translation. In addition, inhibition of cholesterol synthesis with statin resulted in a phenotype similar to that of methionine removal and a decrease in stem cell markers and snoRNA ACA43. Moreover, suppression of FOXM1 decreased stem cell markers such as SOX4 and PROM1. The gene expression profile for cholesterol production was obtained from the Ivy Glioblastoma Atlas Project (IVYGAP) database and compared between tumour cells with relatively low methionine levels in area of pseudopalisading arrangement around necrosis and tumour cells in the infiltrating region, showing that cells cells in the infiltrating region have a higher capacity to produce cholesterol. Taken together, methionine metabolism closely related with self-renew, pluripotency and cell death in GICs through modification of cholesterol biosynthesis: especially SREBF2-FOXM1 and ACA43 axis with modification of ribosomal RNA.
IMT-1 A translational research for practical use of dendritic cell-based immunotherapy against malignant glioma
Authors: Akasaki Yasuharu , Takei Jun , Yamamoto Yohei , Tanaka Toshihide , Kamata Yuko , Murahashi Mutsunori , Murayama Yuichi
Published: December 2021

Background: Although a therapeutic effect of dendritic cell (DC)-based immunotherapy, a kind of regenerative medicine, has been recognized in various types of cancer including malignant glioma, it is still impractical because of several unsolved problems. This study is aimed to solve the problems in regenerative medicine through a clinical trial of immunotherapy using fusions of DCs and glioma cells (GCs) against malignant glioma, and to put it into practical use. Methods: Primary cultured GCs and glioma stem cells (GSCs) were generated from surgical specimens of patient. DCs were generated from PBMC of same patient, and were fused with GCs and GSCs. The entire process of cell production must be performed by pairs of two cell-culture operators in a dedicated cell processing facility. We developed a remote cell-observation system for reducing hands work of operators. As a project to establish a preservation method, cryopreservation of glioma tissues, GCs/GSCs, DCs and fusion cells followed by their viability examination. Results: The remote cell-observation system worked stable in morphological observation and cell-counting for adhesion cells. A growth curve was also automatically and accurately created. Although a morphological observation of floating cells such as GSCs and DCs was possible, there was some error in counting of those cells. A project to establish a preservation method is currently underway, including the development of storage containers and storage liquids. Conclusions: Although the remote cell-observation system required some modifications at the observation site, depth of focus, etc. for floating cells, there was no problem in accuracy for adhesion cells compared with operator’s observation. This system, which can be easily installed at low cost, seemed to be helpful for practical use of regenerative medical products including this therapy. We are working on a project to establish a stable transportation and preservation method for prevalence of this treatment.
MPC-6 Clinical significance of whole chromosomal aberration signatures in non-metastatic medulloblastomas treated with 18Gy of craniospinal irradiation
Authors: Fukuoka Kohei , Kurihara Jun , Mori Makiko , Arakawa Yuki , Yoshioka Ema , Shofuda Tomoko , Matsushita Yuko , Hibiya Yuko , Honda Satoko , Nakazawa Atsuko , Kiyotani Chikako , Kagawa Naoki , Yamasaki Kai , Ando Ryo , Keino Dai , Miyairi Yosuke , Sasaki Atsushi , Nishikawa Ryo , Date Isao , Nagane Motoo , Koh Katsuyoshi , Ichimura Koichi , Kanemura Yonehiro
Published: December 2021

Background: One of the most significant challenges is a reduction in the dose of craniospinal irradiation (CSI) in patients with medulloblastoma to minimize neurological sequelae. However, a North American clinical trial failed to show the prognostic non-inferiority of lower-dose irradiation compared to that associated with standard-dose radiation therapy for non-metastatic medulloblastomas. A European retrospective study revealed that whole chromosomal aberration signatures (WCASs) are a potential prognostic factor in Group 3/4 medulloblastoma without metastasis, but whether the molecular signature has the same clinical impact in patients treated with lower-dose CSI remains unknown. Methods: We conducted DNA methylation analysis using an Illumina Infinium Human Methylation EPIC BeadChip array to investigate molecular prognostic markers in 23 medulloblastoma patients who were registered in the Japan Pediatric Molecular Neuro-Oncology Group and treated with lower-dose CSI relative to standard treatment. A WCAS was defined as the presence of at least two of three chromosomal changes as follows: chromosome (chr) 7 gain, chr 8 loss, and chr 11 gain.Results: All patients presented with no residue or a residual tumor smaller than 1.5 cm2 after surgery without metastasis. The median age at onset was 6.9 years, and the median follow-up period was 80.6 months. CSI was delivered at a median dose of 18.0 Gy. Regarding molecular subgrouping, there were 5 WNT, 2 SHH, 1 Group 3, and 15 Group 4 medulloblastomas. Seven patients with Group 3/4 medulloblastomas showed WCASs and had significantly better prognosis than those without the alteration (5-year progression-free survival 100% vs. 63%, p = 0.046). Two late relapses occurred at 89 and 115 months after diagnosis, respectively, and one of these patients presented with a WCAS.Conclusion: WCAS may be a molecular prognostic marker not only in patients with medulloblastoma treated with standard-dose CSI but also in those treated with lower-dose irradiation.
MPC-17 2021 WHO Classification of Tumors of the CNS, 5th ed
Authors: Komori Takashi
Published: December 2021

The grading of gliomas based on histological features has been a subject of debate for several decades. While the traditional grading system has failed to stratify the risk of IDH-mutant astrocytoma, canonical histological and proliferative markers may be applicable to the risk stratification of IDH-wildtype astrocytoma. Numerous studies have examined molecular markers to obtain more clinically relevant information that will improve the risk stratification of gliomas. The CDKN2A/B homozygous deletion for IDH-mutant astrocytoma and the following three criteria for IDH-wildtype astrocytoma: the concurrent gain of whole chromosome 7 and loss of whole chromosome 10, TERT promoter mutations, and EGFR amplification, were identified as independent molecular markers of the worst clinical outcomes. Therefore, the 2021 World Health Organization (WHO) Classification of Tumors of the Central Nervous System adopted these molecular markers into the revised grading criteria of IDH-mutant and -wildtype astrocytoma respectively, as a grading system within tumor types. For diffuse gliomas in children, molecular alteration-based classification was adopted, dividing low-grade and high-grade subcategories. New tumor types and subtypes were introduced, some based on DNA methylation profiling. To achieve this novel classification in a resource-limited setting, an integrated diagnosis combining clinical, histological, and molecular information became more important.
NQPC-3 A short-time intensive rehabilitation for brain tumor patients with Karnofsky Performance Status of 60-30
Authors: Tejima Sawane , Ueyama Mai , Okamura Daijiro , Ideguchi Makoto , Matsutani Masao
Published: December 2021

OBJECTIVE: Brain tumor patients with KPS of 60 to 30 after the initial treatment are not able to spend independent life at home. The goal of this study is to return these patients to their home with minimal family support by delivering intensive rehabilitation to them. Seventy-five brain patients were evaluated every 10 days from the beginning to the end of rehabilitation treatment, according to clinical scales of Functional Independence Measure (FIM) of 1–7 points depending on the degree of independence. The rehabilitation effect was judged by the degree of improvement of 11 out of 13 motor FIM items, excluding stair climbing and bathing movements. When more than half number of the 11 motor FIM items requiring physical assistance (4 points or less) improved up to non-assistance (5 points or more), it was judged as a significant effect. In addition, when all 11 items present with 6 points (independence possible) or more and all 5 of FIM recognition items are 5 points or more (understand the domestic rules), it was judged that the patients acquired independent living ability. RESULTS: 1. Of the 75 patients, 54 (72%) showed a significant effect, and 38 of them (50.7% of the total) aquired independence at home. The acquisition-rate of independent living ability by tumor was 44.7% for 38 malignant gliomas, 53.8% for 13 metastatic tumors, 50% for 14 meningiomas, and 71.4% for 7 vascular tumors, and there was no significant difference between them. 2. The median time to reach the maximum rehabilitation effect was 35 days. CONCLUSION: Intensive rehabilitation for brain tumor patients with KPS of 60 to 30 is effective and should be incorporated into the palliative treatments in the brain tumor treatment guidelines.
MET-5 Salvage surgical resection after linac-based stereotactic radiosurgery and radiotherapy for brain metastasis
Authors: Matsuda Ryosuke , Morimoto Takayuki , Tamamoto Tetsuro , Kotsugi Masashi , Takeshima Yasuhiro , Tamura Kentaro , Yamada Shuichi , Nishimura Fumihiko , Nakagawa Ichiro , Park Young-Soo , Hasegawa Masatoshi , Nakase Hiroyuki
Published: December 2021

Methods: Between November 2009 and December 2018, 335 consecutive patients with 1085 brain metastases were treated with SRS/fSRT for newly diagnosed brain metastasis at our hospital. Nineteen of 335 patients (5.6%) and 19 of 1044 brain metastases (1.8%) went on to receive SSR after SRS/fSRT during this study period. Two patients underwent multiple surgical resections. Nineteen consecutive patients underwent 21 SSRs. Results: The median time from initial SRS/fSRT to SSR was 14 months (range: 2–96 months). The median follow-up after SSR was 15 months (range: 2–76 months). The range of tumor volume at initial SRS/fSRT was 0.12–21.46 cm3 (median: 2.19 cm3). Histopathological diagnosis after SSR was recurrence, radiation necrosis (RN) and cyst formation in 13 and 6 cases, respectively. The time from SRS/fSRT to SSR were shorter in the recurrence than in the RNs and cyst formation, but these differences did not reach statistical significance (p = 0.07). The median survival time from SSR and from initial SRS/fSRT was 17 months and 74 months, respectively. The cases with recurrence had a significantly shorter survival time from initial SRS/fSRT than those without recurrence (p=0.045).Conclusion: The patients treated with SRS/fSRT for brain metastasis need long-term follow-up. SSR is a safe and effective treatment for the recurrence, RN, and cyst formation after SRS/fSRT for brain metastasis.
Legitimacy of the Suspension of Standing Orders: The Case of the Dewan Rakyat (House of Representatives) of Malaysia
Authors: Saari Muthanna
Published: December 2021

This article explores the ways in which the suspension of standing orders is being practised in the Dewan Rakyat (House of Representatives) of Malaysia during part of the 14th Parliament from 2018 to 2020, with regard to its ultimate goal in achieving legislative functions, rather than as a shortcut to advance legislative or other business. This paper examines the legitimacy of having specific provisions in the Standing Orders of the Dewan Rakyat that would allow a suspension of standing orders to be carried out. Comparable to practices in the Australian and the New Zealand House of Representatives, it is suggested that a clear procedure for the suspension of standing orders in the Dewan Rakyat needs to be devised as part of achieving the proper legislative function of Parliament.
VERA astrometry toward the Perseus arm gap
Authors: Sakai Nobuyuki , Nakanishi Hiroyuki , Kurahara Kohei , Sakai Daisuke , Hachisuka Kazuya , Kim Jeong-Sook , Kameya Osamu
Published: December 2021

The Perseus arm has a gap in Galactic longitudes (l) between 50° and 80° (hereafter the Perseus arm gap) where the arm has little star formation activity. To better understand the gap, we conducted astrometric observations with VERA (VLBI Exploration of Radio Astrometry) and analyzed archival H i data. We report on parallax and proper motion results from four star-forming regions, of which G050.28−00.39 and G070.33+01.59 are likely associated with the gap. The measured parallaxes are 0.140 ± 0.018 (mas), 0.726 ± 0.038 (mas), 0.074 ± 0.037 (mas), and 0.118 ± 0.035 (mas) for G050.28−00.39, G053.14+00.07, G070.33+01.59, and G079.08+01.33, respectively. Since the fractional parallax error of G070.33+01.59 is large (0.5), we estimated a 3D kinematic distance of the source of 7.7 ± 1.0 kpc using both the LSR velocity (VLSR) and the measured proper motion. Perseus-arm sources G049.41+00.32 and G050.28−00.39 lag relative to a Galactic rotation by 77 ± 17 km s−1 and 31 ± 10 km s−1, respectively. The noncircular motion of G049.41+00.32 cannot be explained by the gravitational potential of the Perseus arm. We discovered rectangular holes with integrated brightness temperatures of <30 K arcdeg in l vs. VLSR of the H i data. One of the holes is centered near (l, VLSR) = (47°, −15 km s−1), and G049.41+00.32 is associated with the rim of the hole. However, G050.28−00.39 is not associated with the hole. We found extended H i emission on one side of the Galactic plane when integrating the H i data over the velocity range covering the hole (i.e., VLSR = [−25, −5] km s−1). G049.41+00.32 and G050.28−00.39 are moving toward the emission. The Galactic H i disk at the same velocity range showed an arc structure, indicating that the disk was pushed from the lower side of the disk. All the observational results might be explained by a cloud collision with the Galactic disk.
Untargeted Metabolomic Investigation of Wheat Infected with Stinking Smut Tilletia caries
Authors: Weed Rebecca A. , Savchenko Kyryll G. , Lessin Leandro M. , Carris Lori M. , Gang David R.
Published: December 2021

Tilletia caries infection of wheat (Triticum aestivum) has become an increasing problem in organic wheat agriculture throughout the world. Little is known about how this pathogen alters host metabolism to ensure a successful infection. We investigated how T. caries allocates resources from wheat for its growth over the life cycle of the pathogen. An untargeted metabolomics approach that combined gas chromatography time-of-flight mass spectrometry and ultraperformance liquid chromatography tandem mass spectrometry platforms was used to determine which primary or specialized metabolite pathways are targeted and altered during T. caries infection. We found that T. caries does not dramatically alter the global metabolome of wheat but instead alters key metabolites for its own nutrient uptake and to antagonize host defenses by reducing wheat’s sweet immunity response and other related pathways. Our results highlight metabolic characteristics needed for selecting wheat varieties that are resistant to T. caries infection for organic agriculture. In addition, several wheat metabolites were identified that could be used in developing a diagnostic tool for early detection of T. caries infection.
Fostemsavir: a first-in-class HIV-1 attachment inhibitor
Authors: Grant Philip M. , Kozal Michael J.
Published: December 2021

Purpose of review

Fostemsavir is a recently Food and Drug Administration-approved HIV-1 attachment inhibitor that binds to HIV-1 gp120 and prevents viral attachment to the cellular CD4 receptor. Here, we review the pharmacology, efficacy, tolerability, and resistance profile of fostemsavir.

Recent findings

Fostemsavir is well tolerated and maintains virologic activity in individuals harboring multidrug-resistant HIV-1. In conjunction with optimal background therapy, a majority of heavily treatment-experienced clinical trial participants treated with fostemsavir achieved virologic suppression.

The approval of fostemsavir represents an important advance for individuals harboring multidrug resistant HIV-1 due to its novel mechanism of action and lack of cross-resistance to other antiretrovirals. Further study will better define the role of resistance testing for fostemsavir and fostemsavir's potential role outside of salvage therapy in heavily treatment-experienced individuals.
Lenacapavir: a first-in-class HIV-1 capsid inhibitor
Authors: Dvory-Sobol Hadas , Shaik Naveed , Callebaut Christian , Rhee Martin S.
Published: December 2021

Purpose of review

This review summarizes available data for lenacapavir, an investigational first-in-class agent that disrupts functioning of HIV capsid protein across multiple steps in the viral life cycle.

Recent findings

Lenacapavir demonstrated picomolar potency in vitro with no cross resistance to existing antiretroviral classes and potent antiviral activity in persons with HIV-1. In persons with HIV-1, there was no preexisting resistance to lenacapavir regardless of treatment history. Lenacapavir can be administered orally either daily or weekly and subcutaneously up to every 6 months. In heavily treatment-experienced persons with multidrug-resistant HIV-1 and in treatment-naive persons with HIV-1, lenacapavir in combination with other antiretroviral agents led to high rates of virologic suppression and was well tolerated.

Ongoing studies are evaluating long-acting dosing of lenacapavir for treating HIV-1 in combination with other antiretrovirals and preventing HIV-1 as a single agent.
Risk factors for pulmonary embolism in ICU patients
Authors: Xu Xiujuan , Dai Rongchen , Wu Hanting , Meng Jianbiao , He Peijie , Liang Juan , Ji Conghua
Published: December 2021

Background:

Pulmonary embolism (PE) is a clinical disease of pulmonary hypertension, right heart failure and pulmonary oxygen uptake dysfunction caused by obstruction of pulmonary artery blood flow caused by embolic substances. The incidence rate of acute PE (ATPE) has been increasing year by year in recent years. And ATPE has become a hot topic of common concern in the departments of severe, emergency, respiratory and heart diseases. Research and clinical practice have proved that early thrombolysis and other specific treatment can greatly improve its prognosis. However, the early diagnosis and treatment rate of ATPE is only 7%, for the lack of simple and objective diagnosis and quantitative evaluation of the degree of ATPE. Therefore, the screening of risk factors for PE in intensive care unit (ICU) patients by systematic review and meta-analysis is an important preliminary work to carry out the early diagnosis model of PE.

Methods:

We will retrieve eight electronic databases from their inception to May 31, 2021, which include PubMed, Cochrane Library, Excerpt Medical Database (Embase), Web of science, China Biology Medicine disc, VIP database (VIP), Wan Fang database, China National Knowledge Infrastructure. Methodological quality of all studies was assessed using the Newcastle-Ottawa scale. We will use STATA V.13.0 software and RevMan5.3 for data analysis, and risk factor effect sizes will be expressed as an odds ratio and their 95% Cl.

Results:

We will disseminate the findings of this systematic review and meta-analysis via publications in peer-reviewed journals. With this study, we can get the significant risk factors and risk ratio of PE in ICU patients.

Conclusion:

This study systematically reviewed the existing evidence and determined the risk factors for PE in ICU patients. This study provides a basis for the establishment of early warning and prediction model of PE in ICU patients.