bluuebunny/crossref_metadata_2025 huggingface dataset:
The Human 8-oxoG DNA Glycosylase 1 (OGG1) Ser326Cys Polymorphism in Infertile Men
Authors: González-Díaz César Antonio , Suárez-Souto María Antonieta , Pérez-Soto Elvia , Gómez-López Modesto , Munguía-Cervantes Jacobo Esteban , Pérez-Vielma Nadia Mabel , Sánchez-Monroy Virginia
Published: October 2024

Background/Objectives: 8-hydroxy-2′-deoxyguanosine (8-OHdG) is a form of oxidative DNA damage caused by oxidative stress (OS), which is considered a major factor in male infertility. The cellular defense system against 8-OHdG involves base excision repair (BER) with the enzyme 8-Oxoguanine DNA glycosylase 1 (OGG1). However, studies on the single-nucleotide polymorphism (SNP) OGG1 Ser326Cys have demonstrated that the Cys326Cys genotype could be the cause of an increment in oxidative DNA damage. In this study, the OGG1 Ser326Cys polymorphism and its effect on DNA oxidation were evaluated in 118 infertile men. Methods: Polymorphic screening was performed using TaqMan allelic discrimination assays, and oxidative DNA damage was evaluated through the quantification of 8-OHdG and total antioxidant capacity (TAC); in addition, electrical bioimpedance spectroscopy (EBiS) measurements were used as a reference for different electrical properties associated with 8-OHdG concentrations. Results: The detected Cys (G) allele frequency (0.4) was higher compared to the allele frequency reported in the “Allele Frequency Aggregator” (ALFA) and “Haplotype Map” (HapMap) projects for American populations (0.21–0.29), suggesting that the Cys (G) allele carrier could be a factor associated with American infertile populations. The values of 8-OHdG were twofold higher in carriers of the Cys326Cys (GG) genotype than the other genotypes and, in concordance, the TAC levels were threefold lower in Cys326Cys (GG) genotype carriers compared to the other genotypes. Moreover, the EBiS magnitude exhibited potential for the detection of different oxidative damage in DNA samples between genotypes. Conclusions: The Cys326Cys (GG) genotype is associated with oxidative DNA damage that could contribute to male infertility.
The Therapeutic Effect of Monopolar Radiofrequency Therapy on Urinary Symptoms and Sexual Function
Authors: Long Cheng-Yu , Chang Chieh-Yu , Sung I-Chieh , Loo Zi-Xi , Lin Kun-Ling
Published: October 2024

Objectives: Stress urinary incontinence (SUI) negatively affects the quality of life and sexual function in women. This study aimed to evaluate the efficacy of radiofrequency (RF) therapy in reducing SUI symptoms and its impact on sexual function. Methods: Thirty-four women with SUI were enrolled and underwent a single RF treatment session using the Viveve® System (Viveve Medical Inc., USA) with parameters of 90 J/cm2 and 220 pulses per hour. Assessments at baseline and 6 months post treatment included perineal ultrasound and personal interviews to evaluate lower urinary tract symptoms and sexual function. Urodynamic studies, voiding diaries, and questionnaires such as the Female Sexual Function Index (FSFI), Overactive Bladder Symptom Score (OABSS), Urogenital Distress Inventory-6 (UDI-6), Incontinence Impact Questionnaire-7 (IIQ-7), and International Consultation on Incontinence Questionnaire—Short Form (ICIQ-SF) measured outcomes. Results: RF therapy significantly improved sexual function, with higher FSFI scores in all domains except pain at 6 months. SUI symptoms were significantly reduced, as indicated by improved scores on OABSS, UDI-6, IIQ-7, and ICIQ-SF, alongside better voiding diary results. Anatomical changes included reduced bladder neck mobility, decreased vaginal width, and a reduced rotation angle of the proximal urethra. Conclusions: RF therapy is effective and safe for treating mild to moderate SUI and enhances sexual function, potentially due to changes in vaginal topography. These results suggest RF therapy as a viable non-surgical option for managing SUI and improving sexual health.
Comparative Analysis of Corneal Wound Healing: Differential Molecular Responses in Tears Following PRK, FS-LASIK, and SMILE Procedures
Authors: Janiszewska-Bil Dominika , Grabarek Beniamin Oskar , Lyssek-Boroń Anita , Kiełbasińska Aleksandra , Kuraszewska Bernadeta , Wylęgała Edward , Krysik Katarzyna
Published: October 2024

Background/Objectives: In this study, we aimed to analyze the changes in the expression profiles of selected messenger RNAs (mRNAs) and their encoded proteins in the tears of patients undergoing photorefractive keratectomy (PRK), femtosecond-assisted laser in situ keratomileusis (FS-LASIK), and small-incision lenticule extraction (SMILE) procedures. Methods: A total of 120 patients were divided into three groups based on the laser vision correction (LVC) procedure: PRK, FS-LASIK, or SMILE. Tear samples were collected preoperatively and at 1, 7, 30, and 180 days postoperatively. The expression levels of selected messenger RNAs (mRNAs) and proteins were analyzed by using reverse transcription quantitative polymerase chain reaction (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Results: PRK and FS-LASIK elicited significantly stronger biological responses than SMILE. Interleukin-15 (IL-15) expression increased notably in the PRK and FS-LASIK groups, with mRNA levels reaching fold changes of 4.65 ± 0.65 and 4.99 ± 0.28, respectively, on day 1, compared with only 2.09 ± 0.23 in the SMILE group. Vascular endothelial growth factor A (VEGFA) levels were also elevated in the PRK (2.98 ± 0.23 fold change) and FS-LASIK groups (3.45 ± 1.09 fold change) on day 1, while the SMILE group showed minimal fluctuations. The protein concentration analysis based on the ELISA confirmed these trends, with IL-15 levels peaking at 54.2 ± 2.5 pg/mL in the PRK group and 52.8 ± 3.1 pg/mL in the FS-LASIK group, compared with 32.4 ± 1.9 pg/mL in the SMILE group on day 1. Similarly, VEGFA protein concentrations were the highest in the PRK (72.4 ± 4.1 pg/mL) and FS-LASIK patients (69.5 ± 3.8 pg/mL) on day 1 but remained low in the SMILE patients (45.6 ± 2.3 pg/mL). By day 180, gene expression and protein levels in all groups had stabilized, returning to near-preoperative values. Conclusions: PRK and FS-LASIK induced more pronounced molecular and protein-level changes during corneal wound healing than the less invasive SMILE procedure, indicating stronger biological responses. These findings suggest that tailored postoperative care based on the specific procedure could optimize healing and patient outcomes. However, further research with larger sample sizes and longer follow-ups is needed to confirm these observations and develop personalized treatment strategies.
Compared Antileishmanial Activity of Clomiphene and Tamoxifen
Authors: Sifontes-Rodríguez Sergio , Escalona-Montaño Alma Reyna , Mondragón Flores Ricardo , Mollineda-Diogo Niurka , Monzote Fidalgo Lianet , Mondragón-Castelán Mónica Edith , Alardin-Gutiérrez Fedra , López-Enzana Lourdes Araceli , Sánchez-Almaraz Daniel Andrés , Pérez-Olvera Ofelia , Aguirre-García María Magdalena
Published: October 2024

Drug repositioning is an efficient strategy to search for new treatment alternatives that is especially valuable for neglected parasitic diseases such as leishmaniasis. Tamoxifen and raloxifene are selective estrogen receptor modulators (SERMs) that have shown antileishmanial activity. Clomiphene is a SERM structurally similar to tamoxifen, whose antileishmanial potential is unknown. That is why the objective of the present work was to evaluate its antileishmanial activity in vitro and in vivo in comparison with tamoxifen. The inhibitory effect against promastigotes of L. amazonensis, L. major, and L. mexicana was evaluated for both compounds, as well as the cytotoxicity against mouse peritoneal macrophages, the growth inhibitory activity in intracellular amastigotes of L. mexicana, and the in vivo activity in mice experimentally infected with L. mexicana. Clomiphene was about twice as active as tamoxifen against both promastigotes and intracellular amastigotes, with IC50 values of 1.7–3.3 µM for clomiphene and 2.9–6.4 µM for tamoxifen against all three species of promastigotes and 2.8 ± 0.2 µM and 3.7 ± 0.3 µM, respectively, against L. mexicana amastigotes. Clomiphene structurally affected several parasite organelles in a concentration-dependent fashion, leading to the death of both promastigotes and intracellular amastigotes. Interestingly, the macrophage host cell did not appear damaged by any of the clomiphene concentrations tested. With oral administration at 20 mg/kg for 14 days, both compounds showed similar effects in terms of reducing the growth of the lesions, as well as the weight of the lesions and the parasite load at the end of the follow-up period. The results showed the potential of SERMs as antileishmanial drugs and support further testing of clomiphene and other compounds of this pharmacological group.
Fully Interpretable Deep Learning Model Using IR Thermal Images for Possible Breast Cancer Cases
Authors: Mirasbekov Yerken , Aidossov Nurduman , Mashekova Aigerim , Zarikas Vasilios , Zhao Yong , Ng Eddie Yin Kwee , Midlenko Anna
Published: October 2024

Breast cancer remains a global health problem requiring effective diagnostic methods for early detection, in order to achieve the World Health Organization’s ultimate goal of breast self-examination. A literature review indicates the urgency of improving diagnostic methods and identifies thermography as a promising, cost-effective, non-invasive, adjunctive, and complementary detection method. This research explores the potential of using machine learning techniques, specifically Bayesian networks combined with convolutional neural networks, to improve possible breast cancer diagnosis at early stages. Explainable artificial intelligence aims to clarify the reasoning behind any output of artificial neural network-based models. The proposed integration adds interpretability of the diagnosis, which is particularly significant for a medical diagnosis. We constructed two diagnostic expert models: Model A and Model B. In this research, Model A, combining thermal images after the explainable artificial intelligence process together with medical records, achieved an accuracy of 84.07%, while model B, which also includes a convolutional neural network prediction, achieved an accuracy of 90.93%. These results demonstrate the potential of explainable artificial intelligence to improve possible breast cancer diagnosis, with very high accuracy.
Non-Invasive On–Off Fluorescent Biosensor for Endothelial Cell Detection
Authors: Jiang Qingyun , Shao Shuai , Li Na , Zhang Zhengyao , Liu Bo
Published: October 2024

For rapid and convenient detection of living endothelial cells (ECs) specifically without immunostaining, we developed a biosensor based on turn-on fluorescent protein, named LV-EcpG. It includes a high-affinity peptide E12P obtained through phage display technology for specifically recognizing ECs and a turn-on EGFP fused with two linker peptides. The “on-off” switching mechanism of this genetically encoded fluorescent protein-based biosensor (FPB) ensured that fluorescence signals were activated only when binding with ECs, thus enabling these FPB characters for direct, visual, and non-invasive detection of ECs. Its specificity and multicolor imaging capability established LV-EcpG as a powerful tool for live EC research, with significant potential for diagnosing and treating cardiovascular diseases and tumor angiogenesis.
Review of Liquid Metal Fiber Based Biosensors and Bioelectronics
Authors: Liu Xiaotong , Xu Hui , Li Jiameng , Liu Yanqing , Fan Haojun
Published: October 2024

Liquid metal, as a novel material, has become ideal for the fabrication of flexible conductive fibers and has shown great potential in the field of biomedical sensing. This paper presents a comprehensive review of the unique properties of liquid metals such as gallium-based alloys, including their excellent electrical conductivity, mobility, and biocompatibility. These properties make liquid metals ideal for the fabrication of flexible and malleable biosensors. The article explores common preparation methods for liquid metal conductive fibers, such as internal liquid metal filling, surface printing with liquid metal, and liquid metal coating techniques, and their applications in health monitoring, neural interfaces, and wearable devices. By summarizing and analyzing the current research, this paper aims to reveal the current status and challenges of liquid metal conductive fibers in the field of biosensors and to look forward to their development in the future, which will provide valuable references and insights for researchers in the field of biomedical engineering.
Correction: Chisholm et al. Frontline and Relapsed Rhabdomyosarcoma (FaR-RMS) Clinical Trial: A Report from the European Paediatric Soft Tissue Sarcoma Study Group (EpSSG). Cancers 2024, 16, 998
Authors: Chisholm Julia , Mandeville Henry , Adams Madeleine , Minard-Collin Veronique , Rogers Timothy , Kelsey Anna , Shipley Janet , van Rijn Rick R. , de Vries Isabelle , van Ewijk Roelof , de Keizer Bart , Gatz Susanne A. , Casanova Michela , Hjalgrim Lisa Lyngsie , Firth Charlotte , Wheatley Keith , Kearns Pamela , Liu Wenyu , Kirkham Amanda , Rees Helen , Bisogno Gianni , Wasti Ajla , Wakeling Sara , Heenen Delphine , Tweddle Deborah A. , Merks Johannes H. M. , Jenney Meriel
Published: October 2024

The authors wish to make corrections to the authorship and title of [...]
Green and Sensitive Analysis of the Antihistaminic Drug Pheniramine Maleate and Its Main Toxic Impurity Using UPLC and TLC Methods, Blueness Assessment, and Greenness Assessments
Authors: Abdelhamid Nessreen S. , AlSalem Huda Salem , K. Algethami Faisal , Abdelaleem Eglal A. , Mahmoud Alaa M. , Ella Dalal A. Abou El , Gamal Mohammed
Published: October 2024

For the first time, two direct and eco-friendly chromatographic approaches were adapted for the simultaneous estimation of pheniramine maleate (PAM) and its major toxic impurity, 2-benzyl pyridine (BNZ). Method A used reversed-phase ultra-performance liquid chromatography; separation was achieved within 4 min using a C18 column with a developing system of methanol/water (60:40 v/v) with a 0.1 mL/min flow rate. Photodiode array detection was adjusted at 215 nm. The method was linear in the ranges of 5.0–70.0 and 0.05–10.0 µg/mL for PAM and BNZ, correspondingly. Method B used thin-layer chromatography; separation was applied on silica gel TLC F254 using ethanol/ethyl acetate/liquid ammonia (8:2:0.1, in volumes) at room temperature, at 265 nm. Linearity was assured at concentration ranges 0.5–8.0 and 0.1–3.0 µg/band for the two components, respectively. Generally, the new UPLC and TLC methods outperform the old ones in terms of quickness, greenness, and sensitivity. Concisely, the greenness features were partially achieved using the Green Analytical Procedure Index (GAPI) and the Analytical Greenness (AGREE) pictograms. In contrast, the usefulness of the novel approaches was assured via the Blue Applicability Grade Index (BAGI) tool.
Molar Incisor Hypomineralization and Related Risk Factors among Primary School Children in Jeddah: A Cross-Sectional Study
Authors: Baghlaf Khlood , Bokhari Ghazal Abdulhadi , Aljehani Fatmah Yousef , Shaker Raneem T. , Alshehri Maha , Almushali Abdullah , Alharthi Abdulaziz , Sabbagh Heba Jafar
Published: October 2024

Background/Objectives: Molar Incisor Hypomineralization (MIH) is a prevalent multifactorial developmental dental defect with unclear etiology. This study aimed to identify potential risk factors for MIH among primary school children in Jeddah, Saudi Arabia. Methods: A cross-sectional study including children aged 7 to 10 years old, recruited from twelve randomly selected schools located in three regions of Jeddah city, was carried out. The children were examined for MIH and dental caries using the European Academy of Pediatric Dentistry Criteria (2003) and the DMFT/dmft WHO index. Parents were asked to provide medical and socio-demographic information on their children from their first two years of life. Results: A total of 2010 children were examined, with 888 parents responding to the survey (a 44.2% response rate). Lower maternal education was found to be significantly associated with a decreased adjusted odds ratio (AOR) of MIH (Model 1: p = 0.014, AOR = 0.646, and 95% CI = 0.456 to 0.915; Model 2: p = 0.019, AOR = 0.658, and 95% CI = 0.465 to 0.933). Being an only child, a child’s birth order, type of delivery, breastfeeding, and medical conditions were not associated with MIH. Conclusions: The significant association between MIH and maternal education suggests that further research is needed to explore the underlying mechanisms and identify other potential confounding variables.
The Impact of Socioeconomic Status on Visual Acuity Changes in Schoolchildren: A One-Year Follow-Up
Authors: Galdón Alba , Vila-Vidal Núria , El Gharbi Mariam , Vinuela-Navarro Valldeflors , Pérez-Corral Joan , Tomás Núria , Guisasola Laura
Published: October 2024

(1) Background: Visual acuity (VA) is essential for children’s quality of life, and its relationship with socioeconomic status (SES) highlights disparities in healthcare. This study investigated the influence of SES on changes in schoolchildren’s VA over one year. (2) Methods: Initial examinations were conducted on 1822 children (8–10 years). Follow-up was performed on 804 of these children a year later. Uncorrected (UCVA) and presenting (PVA) distance VA were measured monocularly using a decimal Snellen chart. Very reduced UCVA (<0.5) was considered a proxy of myopia. (3) Results: The prevalence of initially very reduced UCVA (myopia) was similar in children with low and high SES (12.6% vs. 12.4%) (χ2; p = 0.153). After one year, the prevalence of very reduced UCVA increased to 14.1% in children with a low SES compared with 11.1% in children with a high SES (p = 0.001). Significant disparities related to SES were also found in PVA so that children with a low SES exhibited a greater reduction in PVA than children with a high SES (5.2% vs. 3.5%) (χ2; p = 0.004). (4) Conclusions: Children with a low SES showed an increase in reduced UCVA values over one year and a higher number of children with very reduced PVA compared with those with a high SES.
Explainable Boosting Machine Learning for Predicting Bond Strength of FRP Rebars in Ultra High-Performance Concrete
Authors: Mahmoudian Alireza , Bypour Maryam , Kioumarsi Mahdi
Published: October 2024

Aiming at evaluating the bond strength of fiber-reinforced polymer (FRP) rebars in ultra-high-performance concrete (UHPC), boosting machine learning (ML) models have been developed using datasets collected from previous experiments. The considered variables in this study are rebar type and diameter, elastic modulus and tensile strength of rebars, concrete compressive strength and cover, embedment length, and test method. The dataset contains two test methods: pullout tests and beam tests. Four types of rebar, including carbon fiber-reinforced polymer (CFRP), glass fiber-reinforced polymer (GFRP), basalt, and steel rebars, were considered. The boosting ML models applied in this study include AdaBoost, CatBoost, Gradient Boosting, XGBoost, and Hist Gradient Boosting. After hyperparameter tuning, these models demonstrated significant improvements in predictive accuracy, with XGBoost achieving the highest R2 score of 0.95 and the lowest Root Mean Square Error (RMSE) of 2.21. Shapley values analysis revealed that tensile strength, elastic modulus, and embedment length are the most critical factors influencing bond strength. The findings offer valuable insights for applying ML models in predicting bond strength in FRP-reinforced UHPC, providing a practical tool for structural engineering.
Application of Pyroligneous Acid as a Plant Growth Stimulant Can Improve the Nutritional Value of Soybean Seed
Authors: Noel Randi , Schueller Michael J. , Guthrie James , Ferrieri Richard A.
Published: October 2024

Farmers today are using biochemical treatments to improve their crop yields. Commercialized organic biostimulants exist in the form of pyroligneous acid generated by burning agricultural waste products. During the 2023 growing season, we demonstrated that soil treatment with a commercial pyroligneous acid product, Coriphol™, manufactured by Corigin Solutions, Inc., stimulated plant growth and significantly improved yield with an optimal treatment dose of 2 gal. acre−1. In the present work, we examined the effect of this treatment on soybean nutritional content using seed harvested from the 2023 season. Total mean seed protein content for untreated control plants was 32.26 ± 0.49% of dry mass and increased 10.8% to 35.64 ± 0.64% with treatment. This increase resulted in a net reduction in total free amino acid content, although levels of the essential dietary amino acid, lysine, were boosted 6-fold. Total lipid content was unaffected by treatment with mean levels of 21.61 ± 0.70% of dry mass noted. Treatment, however, reduced saturated fatty acid content by roughly 40%, and reduced the polyunsaturated content of linoleic acid in favor of the monounsaturated fatty acid, oleic acid. Finally, Coriphol™ treatment did not impact seed content of eight essential micronutrients including Na, Mg, K, Ca, Fe, Ni, Cu, and Mo, but did significantly boost Zn and Mn levels. Altogether, these results demonstrate that soil treatment with the growth stimulant Coriphol™ has the potential to improve the dietary nutritional value of soybean.
A Comprehensive Review of Moroccan Medicinal Plants for Diabetes Management
Authors: Boutaj Hanane
Published: October 2024

Moroccan flora, renowned for its diverse medicinal plant species, has long been used in traditional medicine to manage diabetes. This review synthesizes ethnobotanical surveys conducted during the last two decades. Among these plants, 10 prominent Moroccan medicinal plants are evaluated for their phytochemical composition and antidiabetic properties through both in vitro and in vivo studies. The review encompasses a comprehensive analysis of the bioactive compounds identified in these plants, including flavonoids, phenolic acids, terpenoids, and alkaloids. Phytochemical investigations revealed a broad spectrum of secondary metabolites contributing to their therapeutic efficacy. In vitro assays demonstrated the significant inhibition of key enzymes α-amylase and α-glucosidase, while in vivo studies highlighted their potential in reducing blood glucose levels and enhancing insulin secretion. Among the ten plants, notable examples include Trigonella foenum-graecum, Nigella Sativa, and Artemisia herba-alba, each showcasing distinct mechanisms of action, such as enzymatic inhibition and the modulation of glucose metabolism pathways. This review underscores the necessity for further chemical, pharmacological, and clinical research to validate the antidiabetic efficacy of these plants and their active compounds, with a view toward their potential integration into therapeutic practices.
A Policy Proposal to Strengthen the Income Redistribution Function of the National Pension Scheme in South Korea: An Analysis of South Korea’s 5th National Pension Comprehensive Plan (Draft)
Authors: Park Jeonyong
Published: October 2024

In this study, we elucidate the income redistribution effects of the proposal to incorporate the Bend Points mechanism of the U.S. OASDI into the Korean National Pension Scheme (BP-KNPS Proposal) through a micro-simulation analysis using individual data from the Korean Labor and Income Panel Study (KLIPS). In addition to examining the effects of introducing the BP-KNPS Proposal into the current National Pension Scheme (NPS), we also consider the impact of this scheme being combined with the 5th National Pension Comprehensive Plan (Draft), announced by Korea’s Ministry of Health and Welfare on 30 October 2023. When the BP-KNPS Proposal is introduced into the current NPS, the Mean Log Deviation (MLD) of the net transfer amount (lifetime pension benefits minus lifetime pension contributions) for regular employees decreases from 0.2022863 to 0.1929960. Similarly, the MLD for self-employed and irregular workers decreases from 0.2046127 to 0.1721433, indicating a reduction in income inequality. Furthermore, when the BP-KNPS Proposal is combined with the 5th National Pension Comprehensive Plan (Draft), the effects are mixed. The proposals to increase the pension contribution rate and adjust the rate increase speed via generation lead to a reduction in income inequality compared to the current NPS when combined with the BP-KNPS Proposal. However, the proposals to raise the pensionable age result in increased income inequality, similar to the outcomes under the current system. This finding suggests that the triple burden identified by literature review—the reduction in benefits due to disparities in contribution periods and life expectancy, and the raised pensionable age—has a greater impact on low-income participants than the inequality-reducing effects of the BP-KNPS Proposal.
Mobilization of Funds of Knowledge in Ecological Environments: Latine Parent Engagement in a College Outreach Program
Authors: Lopez Shannon Lynn , Kiyama Judy Marquez , Sarubbi Molly
Published: October 2024

Latine parent educational engagement literature has established that parents employ rich cultural resources across their environments to support the P-20 attainment of their children. In this qualitative case study, we combine the funds of knowledge framework with constructs of ecological systems theory to add a clearer l perspective of how and with whom Latine parents and communities mobilize their funds of knowledge, highlighting their advocacy and agency. Findings identify instances in which Latine parents navigate different social interactions and spaces at various system levels and demonstrate the ways in which college outreach programs can have positive influences beyond the immediate systems of the home and school.
LCCO2 Assessment and Fertilizer Production from Absorbed-CO2 Solid Matter in a Small-Scale DACCU Plant
Authors: Cheng Tianjiao , Hirota Takeji , Onoda Hiroshi , Pandyaswargo Andante Hadi
Published: October 2024

This study investigates a novel method of utilizing Direct Air Capture (DAC) technology for fertilizer production. Unlike traditional Direct Air Carbon Capture and Utilization (DACCU) technologies, Direct Air Carbon Capture for Fertilizers (FDAC) has the potential to produce fertilizers directly. This study aims to assess the feasibility of FDAC-based fertilizer production by examining the current state of traditional DAC technologies, evaluating the CO2 fixation potential of FDAC, and analyzing the decarbonization effect of producing fertilizers using FDAC. Our evaluation results indicate that CO2 emissions from producing 1 ton of conventional chemical fertilizer, FDAC fertilizer (current status), FDAC fertilizer with ingredient adjustment (sodium hydroxide), and FDAC fertilizer with ingredient adjustment (magnesium hydroxide) are 1.69, 1.12, 1.04, and 1.06 tons of CO2, respectively. The FDAC fertilizer (current status) emits 0.57 tons of CO2 per ton less than commercial fertilizers. FDAC fertilizers also have the potential to reduce CO2 emissions further when the fertilizer composition is adjusted, offering a promising solution for lowering the environmental impact of fertilizer production. Significant CO2 reduction can be expected by replacing conventional low-intensity chemical fertilizers with FDAC-produced fertilizers.
Evaluation of the Energy Management System in Water and Wastewater Utilities in the Context of Sustainable Development—A Case Study
Authors: Machnik-Slomka Joanna , Pawlowska Elżbieta , Klosok-Bazan Iwona , Goňo Miroslava
Published: October 2024

Energy management in enterprises is an important issue in the context of improving energy efficiency, energy use, and energy consumption. This is consistent with the Sustainable Development Goals. The purpose of this study was to evaluate the energy management system of water and wastewater utility in the context of sustainable development based on the opinions of managers and employees. The results indicate the involvement of the surveyed enterprise in energy management system development activity. This demonstrates the orientation of the surveyed enterprise to support activities to improve energy performance in line with the implementation of sustainable development. The added value is that the developed research tool can be used in studies of other enterprises to assess the level of energy management.
MMD-TSC: An Adaptive Multi-Objective Traffic Signal Control for Energy Saving with Traffic Efficiency
Authors: Zhang Yuqi , Zhou Yingying , Wang Beilei , Song Jie
Published: October 2024

Reducing traffic energy consumption is crucial for smart cities, and vehicle carbon emissions are a key energy indicator. Traffic signal control (TSC) is a useful method because it can affect the energy consumption of vehicles on the road by controlling the stop-and-go of vehicles at traffic intersections. However, setting traffic signals to reduce energy consumption will affect traffic efficiency and this is not in line with traffic management objectives. Current studies adopt multi-objective optimization methods with high traffic efficiency and low carbon emissions to solve this problem. However, most methods use static weights, which cannot adapt to complex and dynamic traffic states, resulting in non-optimal performance. Current energy indicators for urban transportation often fail to consider passenger fairness. This fairness is significant because the purpose of urban transportation is to serve people’s mobility needs not vehicles. Therefore, this paper proposes Multi-objective Adaptive Meta-DQN TSC (MMD-TSC), which introduces a dynamic weight adaptation mechanism to simultaneously optimize traffic efficiency and energy saving, and incorporates the per capita carbon emissions as the energy indicator. Firstly, this paper integrates traffic state data such as vehicle positions, velocities, vehicle types, and the number of passengers and incorporates fairness into the energy indicators, using per capita carbon emissions as the target for reducing energy consumption. Then, it proposes MMD-TSC with dynamic weights between energy consumption and traffic efficiency as reward functions. The MMD-TSC model includes two agents, the TSC agent and the weight agent, which are responsible for traffic signal adjustment and weight calculation, respectively. The weights are calculated by a function of traffic states. Finally, the paper describes the design of the MMD-TSC model learning algorithm and uses a SUMO (Simulation of Urban Mobility) v.1.20.0 for traffic simulation. The results show that in non-highly congested traffic states, the MMD-TSC model has higher traffic efficiency and lower energy consumption compared to static multi-objective TSC models and single-objective TSC models, and can adaptively achieve traffic management objectives. Compared with using vehicle average carbon emissions as the energy consumption indicator, using per capita carbon emissions achieves Pareto improvements in traffic efficiency and energy consumption indicators. The energy utilization efficiency of the MMD-TSC model is improved by 35% compared to the fixed-time TSC.
Modular Microgrid Technology with a Single Development Environment Per Life Cycle
Authors: Mîndra Teodora , Chenaru Oana , Dobrescu Radu , Toma Lucian
Published: October 2024

The life cycle of a microgrid covers all the stages from idea to implementation, through exploitation until the end of its life, with a lifespan of around 25 years. Covering them usually requires several software tools, which can make the integration of results from different stages difficult and may imply costs being hard to estimate from the beginning of a project. This paper proposes a unified platform composed of four modules developed in MATLAB 2022b, designed to assist all the processes a microgrid passes through during its lifetime. This entire platform can be used by a user with low IT knowledge, because it is completed with fill-in-the-blank alone, as a major advantage. The authors detail the architecture, functions and development of the platform, either by highlighting the novel integration of existing MATLAB tools or by developing new ones and designing new user interfaces linked with scripts based on its complex mathematical libraries. By consolidating processes into a single platform, the proposed solution enhances integration, reduces complexity and provides better cost predictability throughout the project’s duration. A proof-of-concept for this platform was presented by applying the life-cycle assessment process on a real-case study, a microgrid consisting of a photovoltaic plant, and an office building as the consumer and energy storage units. This platform has also been developed by involving students within summer internships, as a process strengthening the cooperation between industry and academia. Being an open-source application, the platform will be used within the educational process, where the students will have the possibility to add functionalities, improve the graphical representation, create new reports, etc.
Associations between Circulating Biomarkers of One-Carbon Metabolism and Mitochondrial D-Loop Region Methylation Levels
Authors: Stoccoro Andrea , Lari Martina , Migliore Lucia , Coppedè Fabio
Published: October 2024

Background/Objectives: One-carbon metabolism is a critical pathway for epigenetic mechanisms. Circulating biomarkers of one-carbon metabolism have been associated with changes in nuclear DNA methylation levels in individuals affected by age-related diseases. More and more studies are showing that even mitochondrial DNA (mtDNA) could be methylated. In particular, methylation of the mitochondrial displacement (D-loop) region modulates the gene expression and replication of mtDNA and, when altered, can contribute to the development of human illnesses. However, no study until now has demonstrated an association between circulating biomarkers of one-carbon metabolism and D-loop methylation levels. Methods: In the study presented herein, we searched for associations between circulating one-carbon metabolism biomarkers, including folate, homocysteine, and vitamin B12, and the methylation levels of the D-loop region in DNA obtained from the peripheral blood of 94 elderly voluntary subjects. Results: We observed a positive correlation between D-loop methylation and vitamin B12 (r = 0.21; p = 0.03), while no significant correlation was observed with folate (r = 0.02; p = 0.80) or homocysteine levels (r = 0.02; p = 0.82). Moreover, D-loop methylation was increased in individuals with high vitamin B12 levels compared to those with normal vitamin B12 levels (p = 0.04). Conclusions: This is the first study suggesting an association between vitamin B12 circulating levels and mtDNA methylation in human subjects. Given the potential implications of altered one-carbon metabolism and mitochondrial epigenetics in human diseases, a deeper understanding of their interaction could inspire novel interventions with beneficial effects for human health.
A Wind Tunnel Test for the Effect of Seed Tree Arrangement on Wake Wind Speed
Authors: Yoon Tae Kyung , Lee Seonghun , Lee Seungmin , Lee Sle-gee , Hussain Mariam , Lee Seungho , Chung Haegeun , Chung Sanghoon
Published: October 2024

Changes in canopy structures caused by harvesting and regeneration practices can significantly alter the wind environment. Therefore, it is essential to understand the wind patterns influenced by seed tree arrangements for predicting seed dispersal by winds and ensuring the success of natural regeneration. This study aimed to identify how wind speed responds to seed tree arrangement designs with differing horizontal distances, vertical positions, and free-stream wind speeds. A wind tunnel test was conducted using pine saplings for a scale model of various seed tree arrangements, and the change in wake speed was tracked. The wake’s relative wind speed averaged 71%, ranging from 3.5% to 108.5%, depending on the seed tree arrangement, distance from saplings, and vertical position. It peaked within the patch of three seed trees compared to other arrangements and at the top canopy layer. The empirical function effectively described the wind speed reduction and recovery with distance from saplings. For instance, the minimum wind speed was reached at 0.6–2.2 times the canopy height, and a wind speed reduction of over 20% of the free-stream wind speed was maintained at a 1.6–7.6 canopy height. A negative relationship between the seed tree leaf area and the relative wind speed was observed only at the top canopy layer. This study presents empirical evidence on the patterns of wake winds induced by different types of heterogeneous canopy structures.
ALS-Based, Automated, Single-Tree 3D Reconstruction and Parameter Extraction Modeling
Authors: Wang Hong , Li Dan , Duan Jiaqi , Sun Peng
Published: October 2024

The 3D reconstruction of point cloud trees and the acquisition of stand factors are key to supporting forestry regulation and urban planning. However, the two are usually independent modules in existing studies. In this work, we extended the AdTree method for 3D modeling of trees by adding a quantitative analysis capability to acquire stand factors. We used unmanned aircraft LiDAR (ALS) data as the raw data for this study. After denoising the data and segmenting the single trees, we obtained the single-tree samples needed for this study and produced our own single-tree sample dataset. The scanned tree point cloud was reconstructed in three dimensions in terms of geometry and topology, and important stand parameters in forestry were extracted. This improvement in the quantification of model parameters significantly improves the utility of the original point cloud tree reconstruction algorithm and increases its ability for quantitative analysis. The tree parameters obtained by this improved model were validated on 82 camphor pine trees sampled from the Northeast Forestry University forest. In a controlled experiment with the same field-measured parameters, the root mean square errors (RMSEs) and coefficients of determination (R2s) for diameters at breast height (DBHs) and crown widths (CWs) were 4.1 cm and 0.63, and 0.61 m and 0.74, and the RMSEs and coefficients of determination (R2s) for heights at tree height (THs) and crown base heights (CBHs) were 0.55 m and 0.85, and 1.02 m and 0.88, respectively. The overall effect of the canopy volume extracted based on the alpha shape is closest to the original point cloud and best estimated when alpha = 0.3.
Vapor-Phase Essential Oils as Antifungal Agents against Penicillium olsonii Causing Postharvest Cherry Tomato Rot
Authors: Mrvová Monika , Medo Juraj , Lakatošová Jana , Barboráková Zuzana , Golian Marcel , Mašková Zuzana , Tančinová Dana
Published: October 2024

Recent reports of P. olsonii causing postharvest rot of cherry tomatoes emphasize the need for effective strategies to prolong fruit shelf life. This study is the first to explore the use of essential oils (EOs), recognized for their antimicrobial properties, as a potential method to prevent postharvest losses from P. olsonii. Antifungal activity was tested for ten EOs at a concentration of 625 μL/L using the vapor diffusion method. Thyme, wild thyme, savory, oregano, and marjoram completely inhibited fungal growth over 14 days. Thyme EO, at a minimum inhibitory concentration (MIC) of 250 μL/L, fully inhibited all strains, while oregano, wild thyme, and savory were effective at 500 μL/L. Marjoram EO showed weaker activity. The lowest IC90 values, ranging from 35.72 to 162.72 μL/L, were estimated for thyme and oregano. In cherry tomatoes, oregano EO completely halted P. olsonii growth at 250 μL/L; thyme was effective for seven days; wild thyme and savory for two days. Thyme EO prevented P. olsonii spore germination at 500 μL/L for seven days, though germination occurred at half that concentration. The IC90 values varied between 256.2 and 138.7 μL/L depending on the strain. The vapor phase of EOs at 125 μL/L influenced the sensory characteristics of cherry tomatoes; however, for thyme and oregano, this effect was not negative due to their culinary association with tomato flavor. The selected EOs could be used to control and prevent postharvest fruit losses, but further research is needed to optimize their application.
Meat Inspection Decisions Regarding Pig Carcasses Affected by Osteomyelitis at the Slaughterhouse: From Etiopathogenesis to Total Condemnation Criteria
Authors: Rodrigues Melissa Alves , Teiga-Teixeira Pedro , Seixas Fernanda , Esteves Alexandra
Published: October 2024

Osteomyelitis is a significant cause of total carcass condemnation in pigs at the slaughterhouse. The decision for total condemnation of a pig carcass for osteomyelitis is often based on traditional perceptions of the risk of pyaemia, leading to controversy among Official Veterinarians (OV) in the industry. This review aims to provide a more comprehensive understanding of the etiopathogenesis of osteomyelitis in pigs, the microorganisms involved, and the risk factors. It also highlights the urgent need for a more uniform method to evaluate osteomyelitis cases, which could significantly reduce economic losses in the industry. Lesions originating from tail-biting, tail docking, castration, teeth resection, and raw management are described as risk factors for osteomyelitis. Osteomyelitis is caused by the entry of pathogens into the animal’s bloodstream through an open wound. Trueperella monocytogenes, Staphylococcus aureus, and Streptococcus spp. are the most described pathogens. At slaughter, OVs condemn carcasses with osteomyelitis due to pyaemia. Signs of acute disease are essential to identify pyaemia cases. In chronic cases, total carcass condemnation can be avoided depending on the number of lesions and vertebrae affected. A clear overall image of the problem would help authorities in various countries adopt a more homogenous approach.
Acceleration of Ethanol Metabolism by a Patented Bos taurus Isolated Alcohol Degradation Protein (ADP) on Acute Alcohol Consumption
Authors: Tsoi Bun , Zhang Huan , So Chun-Pang , Lam Angel Ka-Kei , Poon Christina Chui-Wa , Law Sek-Lun , Wong Bing-Lou , Seto Sai-Wang
Published: October 2024

Alcoholic beverages are among the most widely enjoyed leisure drinks around the world. However, irresponsible drinking habits can have detrimental effects on human health. Therefore, exploring strategies to alleviate discomfort following alcohol consumption would be beneficial for individuals who inevitably need to consume alcohol. In this study, three different models were used to determine the efficacy of a patented alcohol degradation protein (ADP) extracted from Bos taurus on ethanol metabolism. In an ethanol-challenged HepG2 cell model, ADP significantly protected the cell from ethanol-induced toxicity. Subsequently, results demonstrated that ADP significantly alleviated the effect of ethanol, as reflected by the increased distance and activity time of zebrafish during the testing period. Additionally, in a rat model, ADP promoted ethanol degradation at 1 and 2 h after ethanol consumption. Mechanistic studies found that ADP treatment increased ADH and ALDH activity in the gastrointestinal tract. ADP also exhibited potent antioxidation effects by lowering HO-1 expression in the liver. In conclusion, we believe that ADP is a promising product for relieving hangover symptoms after ethanol consumption, with demonstrated safety and effectiveness at the recommended dosage.
Oral Administration of Lactiplantibacillus plantarum CCFM8661 Alleviates Dichlorvos-Induced Toxicity in Mice
Authors: Ma Weiwei , Zhao Yiyang , Sun Hang , Zhang Ziwei , Huang Lili
Published: October 2024

Dichlorvos (DDVP) is an organophosphorus pesticide commonly used in agriculture for pest control, which may enter the organism from the food chain and cause harm. This study aimed to investigate the mitigation effect of Lactiplantibacillus plantarum CCFM8661 (a strain of the bacteria) on DDVP toxicity. Sixty male mice were randomly divided into five groups including control (saline), model (DDVP), low-dose, medium-dose, and high-dose groups, and alleviating effect was evaluated by determining body weight, pesticide residues, oxidative stress, and inflammation, and by histological analysis. The results showed that compared with the model group, body weight and acetylcholinesterase activity, and SOD, CAT, T-AOC, and GSH levels significantly increased, and serum DDVP content, MDA level, IL-1β, and TNF-α significantly decreased after administration of the L. plantarum CCFM8661. The study demonstrated that L. plantarum CCFM8661 exhibited a significant detoxification effect on pesticide toxicity in mice, providing a theoretical basis for the application of probiotics in mitigating pesticide-induced damage.
Development and Validation of a Questionnaire to Assess the Radiologists’ Views on the Implementation of Artificial Intelligence in Radiology (ATRAI-14)
Authors: Vasilev Yuriy A. , Vladzymyrskyy Anton V. , Alymova Yulya A. , Akhmedzyanova Dina A. , Blokhin Ivan A. , Romanenko Maria O. , Seradzhi Seal R. , Suchilova Maria M. , Shumskaya Yuliya F. , Reshetnikov Roman V.
Published: October 2024

Introduction: Artificial Intelligence (AI) is becoming an essential part of modern radiology. However, available evidence highlights issues in the real-world applicability of AI tools and mixed radiologists’ acceptance. We aimed to develop and validate a questionnaire to evaluate the attitude of radiologists toward radiology AI (ATRAI-14). Materials and Methods: We generated items based on the European Society of Radiology questionnaire. Item reduction yielded 23 items, 12 of which contribute to scoring. The items were allocated into four domains (“Familiarity”, “Trust”, “Implementation Perspective”, and “Hopes and Fears”) and a part related to the respondent’s demographics and professional background. As a pre-test method, we conducted cognitive interviews with 20 radiologists. Pilot testing with reliability and validity assessment was carried out on a representative sample of 90 respondents. Construct validity was assessed via confirmatory factor analysis (CFA). Results: CFA confirmed the feasibility of four domains structure. ATRAI-14 demonstrated acceptable internal consistency (Cronbach’s Alpha 0.78 95%CI [0.68, 0.83]), good test–retest reliability (ICC = 0.89, 95% CI [0.67, 0.96], p-value < 0.05), and acceptable criterion validity (Spearman’s rho 0.73, p-value < 0.001). Conclusions: The questionnaire is useful for providing detailed AI acceptance measurements for making management decisions when implementing AI in radiology.
Functional and Proteomic Analyses of a Putative Carbamoyl Phosphate Synthase Large Subunit in Relation to Virulence, Arginine and Pyrimidine Biosynthesis, and Siderophore Production in Erwinia amylovora
Authors: Han Yoobin , Lee Seung Yeup , Kim Dohyun , Lee Suhyun , Choi Junhyeok , Cho Yongmin , Lee Jeongwook , Roh Eunjung , Han Sang-Wook
Published: October 2024

The apple is a significant global fruit cultivated extensively worldwide. Fire blight, caused by Erwinia amylovora (Ea), poses a significant threat to global apple production. To control this disease, characterizing the virulence mechanisms/factors is imperative. Carbamoyl phosphate synthase is an essential enzyme in the biosynthesis of arginine and pyrimidine. However, the functions of this protein in Ea remains poorly understood. This study aimed to investigate the functions of the carbamoyl phosphate synthase large subunit in Ea (CarBEa). In a virulence assay using fruitlets, an Ea strain lacking CarBEa exhibited significantly reduced virulence on fruitlets. In the auxotrophy assay, this mutant failed to grow in minimal media lacking both arginine and pyrimidine, but growth was restored when both compounds were supplemented. The comparative proteomic analysis suggests that CarBEa is involved in diverse biological processes, including amino acid and nucleotide metabolism, and inorganic ion transport. Finally, we demonstrated that CarBEa is related to siderophore secretion/production by the chrome azurol S agar plate assay. This report provides valuable insights into the functions of carbamoyl phosphate synthase large subunit, which serves as a potential target for developing efficient anti-virulence substances to control fire blight.