bluuebunny/crossref_metadata_2025 huggingface dataset:
FOLFIRI-bevacizumab as a second-line treatment for advanced biliary tract cancer after gemcitabine-based chemotherapy
Authors: Roussot Nicolas , Vincent Julie , Palmier Remi , Constantin Guillaume , Bengrine Leila , Fumet Jean-David , Ghiringhelli François
Published: November 2023

Background

Advanced biliary tract cancer (BTC) has a poor prognosis. Gemcitabine with platinum chemotherapy was the standard first-line chemotherapeutic regimen until the recent addition of anti-PD-1/PD-L1 antibodies. After disease progression, the only second-line chemotherapy that has demonstrated a survival benefit versus supportive care is FOLFOX (folinic acid, fluorouracil, and oxaliplatin), with a modest benefit. This study aimed to assess the efficacy and safety of second-line FOLFIRI (folinic acid, fluorouracil, and irinotecan) combined with bevacizumab for advanced BTC.

Methods

This single-center retrospective study enrolled patients with metastatic BTC (intrahepatic cholangiocarcinoma [ICC], extrahepatic cholangiocarcinoma [ECC], or gallbladder carcinoma) that progressed after first-line gemcitabine-based chemotherapy. FOLFIRI-bevacizumab was administered intravenously every 2 weeks [folinic acid 200 mg/m², fluorouracil 400 mg/m² (bolus), fluorouracil 2400 mg/m² (46-h continuous intravenous infusion), irinotecan 180 mg/m², and bevacizumab 5 mg/kg] until unacceptable toxicity, patient refusal, or disease progression.

Results

Overall, 28 patients received the FOLFIRI-bevacizumab regimen after gemcitabine-based chemotherapy. The median overall survival (OS) was 9.0 months (95% CI 6.4–16.5). The OS rate was 39.3% (95% CI 24.8–62.3) and 10.7% (95% CI 3.7–32.1) at 12- and 24-months respectively. The median progression-free survival (PFS) was 5.2 months (95% CI 3.1–10.2) with FOLFIRI-bevacizumab. The PFS rates at 12 months and 24 months were 17.9% (95% CI 8.19–39.5] and 10.7% (95% CI 3.7–31.2), respectively. The overall response rate (ORR) to FOLFIRI-bevacizumab was 23.1%, with a disease control rate (DCR) of 69.3%. Grade 3-4 adverse events (sAE) were reported in 20 patients (71.4%) treated with FOLFIRI-bevacizumab.

Conclusion

FOLFIRI-bevacizumab as a second-line treatment for advanced BTC after gemcitabine-based chemotherapy showed efficacy and safety with a promising tumor response rate in this retrospective single-center study.
Fruit quality assessment based on mineral elements and juice properties in nine citrus cultivars
Authors: Jiao Yiling , Zhang Shuozhen , Jin Haitao , Wang Yuwen , Jia Yamin , Zhang Hua , Jiang Yuying , Liao Wenqiang , Chen Li-Song , Guo Jiuxin
Published: November 2023

Introduction

Citrus fruit is considered a superfood due to its multiple nutritional functions and health benefits. Quantitative analysis of the numerous quality characteristics of citrus fruit is required to promote its sustainable production and industrial utilization. However, little information is available on the comprehensive quality assessment of various fruit quality indicators in different citrus cultivars.

Methods

A total of nine different fresh citrus fruits containing seeds were collected as the experimental materials. The objectives of this study were: (i) to determine the morphological and juice properties of citrus fruits, (ii) to measure the mineral elements in the peel, pulp, and seeds, and (iii) to evaluate the fruit quality index (FQI) using the integrated quality index (IQI) and the Nemoro quality index (NQI) methods.

Results

There were significant differences in fruit quality characteristics, including morphological, mineral, and juice quality, among the investigated citrus cultivars. The proportion of pulp biomass was the highest, followed by that of peel and seeds. N and Cu had the highest and lowest concentrations, respectively, among the measured elements across all citrus fruits, and the amounts of N, P, Mg, Cu, and Zn in seeds, K and Al in pulp, and Ca, Fe, and Mn in peel were the highest, dramatically affecting the accumulation of minerals in the whole fruit and their distribution in various fruit parts. Additionally, Ningmeng fruits had the highest vitamin C and titratable acidity (TA) but the lowest total soluble solids (TSS) and total phenolic (TP) contents, resulting in the lowest TSS/TA and pH values. In contrast, Jinju fruits had the highest TSS and TP contents. Based on the mineral element and juice quality parameters, principal component analysis showed that the citrus fruits were well separated into four groups, and the dendrogram also showed four clusters with different distances. The FQI range based on the IQI method (FQIIQI) and NQI method (FQINQI) was 0.382-0.590 and 0.106-0.245, respectively, and a positive relationship between FQIIQI and FQINQI was observed.

Conclusion

Our results highlight the great differences in mineral and juice characteristics among fruit parts, which mediated fruit quality. The strategy of fruit quality assessment using the FQI can be expanded for targeted utilization in the citrus industry.
Auditory discomfort in visually sensitive individuals
Authors: Haigh Sarah M. , Haugland Anna M. , Mendoza Lourdes R. , Montero Mackenzie
Published: November 2023

Introduction

Sensory discomfort occurs in clinical and non-clinical populations. While some of the parameters that evoke visual discomfort have been identified, the parameters of sounds that evoke auditory discomfort are largely unknown.

Methods

We presented various sounds and asked participants to rate the discomfort they experienced. In Experiments 1 and 2 tones were presented at frequencies between 0.25-8 kHz and modulated sinusoidally in amplitude at frequencies between 0-32 Hz. In Experiment 3 tones were swept in frequency from 500 Hz-2 kHz at sweep rates of 5-50 per second. In Experiment 4, sweeps varied in frequency range and central frequency.

Results

Discomfort increased with frequency. The effects of the amplitude modulation and sweep rate on discomfort were relatively small and were experienced mainly at low modulation frequencies and high sweep rates. Individuals who experienced visuo-perceptual distortions in the Pattern Glare (PG) Test reported greater auditory discomfort.

Discussion

This suggests that sensory sensitivity in one modality may occur in another.
Maladaptive cognitive regulation moderates the mediating role of emotion dysregulation on the association between psychosocial factors and non-suicidal self-injury in depression
Authors: Ge Yuqi , Xiao Yang , Li Mingzhu , Yang Lei , Song Peihua , Li Xueni , Yan Hao
Published: November 2023

Introduction

Non-suicidal self-injury (NSSI) is highly prevalent in depression, and is associated with psychosocial factors, emotion dysregulation, and strategies of cognitive emotion regulation. However, the internal combination and interactions of these risk factors in depression remain unclear.

Methods

Data from 122 patients with depression, including 56 with NSSI and 66 without NSSI, were analyzed. Self-rating scales were used to assess psychosocial factors, emotion dysregulation, and cognitive regulation strategies. Sparse partial least squares discriminant analysis (sPLS-DA) was employed to explore internal combinations in each profile. A moderated mediation model was applied to examine their interactional relationship.

Results

The results identified an NSSI-related psychosocial profile characterized by high neuroticism, childhood trauma, poor family functioning, and low psychological resilience. Emotion dysregulation, including high levels of alexithymia, anhedonia, and emotion regulation difficulties, mediated the association between this psychosocial profile and NSSI. The mediated effect was further moderated by maladaptive cognitive regulation strategies.

Limitations

Lack of sufficient information on NSSI frequency and severity. Relatively small sample size for discussing the impact of gender and age of depressive patients with NSSI.

Conclusion

These findings hold important implications for the prevention, treatment, and rehabilitation of NSSI.
Unfolding COVID-19 vaccine communication campaigns in China’s neighborhoods: a qualitative study of stakeholders’ narratives
Authors: Yang Ronghui , Han Yanchao
Published: November 2023

Introduction

The Chinese state has recently implemented the COVID-19 Vaccine Communication Campaign (CVCC) to counter vaccine hesitancy. Nonetheless, the extant literature that examines COVID-19 vaccine acceptance has less represented COVID-19 vaccine communication efforts.

Methods

To address this lacuna, we qualitatively explored how CVCCs were organized in Chinese communities by investigating 54 Chinese stakeholders.

Results

This study indicates that the CVCC was sustained by top-down political pressure. CVCCs’ components involve ideological education among politically affiliated health workers, expanding health worker networks, training health workers, implementing media promotion, communicating with residents using persuasive and explanatory techniques, encouraging multistakeholder partnerships, and using public opinion-steered and coercive approaches. While CVCCs significantly enhanced COVID-19 vaccine acceptance, lacking open communication, stigmatizing vaccine refusers, insufficient stakeholder collaboration, and low trust in the COVID-19 vaccination program (CVP) eroded the validity of CVCCs.

Discussion

To promote the continuity of CVCCs in China, CVCC performers are expected to conduct open and inclusive communication with residents. Furthermore, CVP planers should create robust partnerships among health workers by ensuring their agreements on strategies for implementing CVCCs and optimize COVID-19 immunization service provision to depoliticize CVPs. Our study will not only deepen global audiences’ understanding of CVCCs in authoritarian China but also offer potential neighborhood-level solutions for implementing local and global public health communication efforts.
Advancing neurosurgical education in the age of online learning and global knowledge sharing: impact of Cerebrovascular Q&A webinar series
Authors: Faisal Umme Habiba , Idrissi Yassine Alami , Chaurasia Bipin , Takasumi Alexis , Baldoncini Matias , Patel Akshal , Monteith Stephen , McDougall Cameron , Costa Matias
Published: November 2023

Background

The Seattle Science Foundation created the Cerebrovascular Q&A series as a free web-based tool to educate physicians and physicians-in-training about cerebrovascular and endovascular neurosurgery across geographical boundaries and different levels of training.

Objective

This study aims to assess the educational impact and clinical implications of the Cerebrovascular Q&A webinar series, hosted by the Seattle Science Foundation.

Methods

A digital anonymous, self-administered survey was sent to the live webinar participants. The survey contained questions about the socio-demographic characteristics of the participants, their perception of the content of the webinar series, and its impact on academic and clinical practice. The data collected from the Survey-Monkey platform was exported to Microsoft Excel which was used to perform all statistical analyses. The viewer metrics on Zoom and YouTube were also analyzed to understand trends observed among a diverse global cohort of participants.

Result

A total of 2,057 people hailing from 141 countries had registered for the Cerebrovascular Q&A series. The response rate to the questionnaire was 12.63% (n = 260). Respondents hailed from 65 countries, of which the majority were from India (13.46%, n = 35) and United States (11.15%, n = 29). Most of the participants were male (82.69%, n = 215), while only 15.77% (n = 41) were female. The maximum number of participants were neurosurgery attendings (36.65%, n = 92) followed by neurosurgeons undergoing fellowship training (24.70%, n = 62) and students who were currently in residency training (15.54%, n = 39). 75.97% (n = 196) heard of the Cerebrovascular Q&A series through the emails from Seattle Science Foundation. 21.5% (n = 56) learned about the webinar series through social media. 75% of participants reported that the webinar content was advanced and comprehensive, and the selection of speakers was relevant. 63.08% (n = 164) found the webinars sparked innovative research ideas. Additionally, 55% (n = 143) reported changes in their clinical practice based on the acquired knowledge.

Conclusion

The findings from this study reveal that webinar-based medical education in cerebrovascular neurosurgery is highly effective and influential. Web-based platforms and social media present a potent strategy to overcome barriers, emphasizing the need for targeted efforts to engage more women in medicine and neurosurgery recruitment.
Evaluating temporal bone column density for optimized bone conduction implant placement
Authors: Talon Emile , Wagner Franca , Weder Stefan , Anschuetz Lukas , Caversaccio Marco , Wimmer Wilhelm
Published: November 2023

Introduction

An optimal placement of bone conduction implants can provide more efficient mechanical transmission to the cochlea if placed in regions with greater bone column density. The aim of this study was to test this hypothesis and to determine the clinical potential of preoperative bone column density assessment for optimal implant placement.

Methods

Five complete cadaver heads were scanned with quantitative computed tomography imaging to create topographic maps of bone density based on the column density index (CODI). Laser Doppler vibrometry was used to measure cochlear promontory acceleration under bone conduction stimulation in different locations on the temporal bone, using a bone-anchored hearing aid transducer at frequencies ranging from 355 Hz to 10 kHz.

Results

We found a statistically significant association between CODI levels and the accelerance of the cochlear promontory throughout the frequency spectrum, with an average increase of 0.6 dB per unit of CODI. The distance between the transducer and the cochlear promontory had no statistically significant effect on the overall spectrum.

Discussion

We highlight the importance of bone column density in relation to the mechanical transmission efficiency of bone conduction implants. It may be worthwhile to consider column density in preoperative planning in clinical practice.
Modular Multifunctional Composite Structure for CubeSat Applications: Embedded Battery Prototype Modal Analysis
Authors: Capovilla Giorgio , Cestino Enrico , Reyneri Leonardo
Published: November 2023

Current CubeSats usually exhibit a low structural mass efficiency and a low internal volume for their payloads. The present work aims to propose an advanced structural architecture for CubeSats that addresses the issues of low structural mass efficiency and payload volume. The starting concept is the smart tiles architecture for satellites developed for the ARAMIS (an Italian acronym for a highly modular architecture for satellite infrastructures) CubeSat project. By introducing multifunctional structures and lightweight, composite materials in the design of smart tiles, the volumetric and structural mass efficiency of the entire CubeSat are enhanced. The advantages of the chosen approach are preliminarily analyzed in terms of the volumetric efficiency and amplitude of the payload design space. A 1U battery tile design is then selected to investigate the multifunctional structures design aspects in the project of space structures. A battery tile prototype is designed, produced, and tested. The CubeSat volumetric increment and the payload volume gain with respect to the traditional architecture is shown to reach a maximum of 37%. The CubeSat structural mass ratio can be reduced to 16.7%.
Dynamic Modeling and Vibration Suppression of a Rotating Flexible Beam with Segmented Active Constrained Layer Damping Treatment
Authors: Wang Yue , Fang Yiming , Li Liang , Zhang Dingguo , Liao Wei-Hsin , Fang Jianshi
Published: November 2023

This paper uses high-order approximate coupling (HOAC) dynamics equations for the hub–beam system with segmented active constrained layer damping treatment (SACLD). To improve the damping characteristics of traditional active constrained layer damping (ACLD), the viscoelastic damping layer, and the piezoelectric constraining layer are cut at the same position. The damping characteristics of the structure are enhanced by increasing the shear strain of the viscoelastic damping layer. The finite element method is used to discretize the SACLD beam. The discontinuity of the SACLD beam element-to-element displacement achieves the notch. Based on the theory of rigid–flexible coupling dynamics, the dynamic responses of the SACLD rotating beam under different cases are studied. The results show that the segmentation method is not always effective. A SACLD beam provides better vibration suppression than an ACLD beam only when appropriate material and dimensional parameters are used. The influences of base-layer thickness, piezoelectric constraining layer thickness, viscoelastic damping-layer thickness, angular velocity, the viscoelastic damping-layer loss factor, and control gains on the vibration of the rotating flexible beam with SACLD treatment are also discussed.
Will the Grain Imports Competition Effect Reverse Land Green Efficiency of Grain Production? Analysis Based on Virtual Land Trade Perspective
Authors: Ye Weijiao , Li Ziqiang
Published: November 2023

To explore new strategies to improve the efficiency of land for grain production in China, this study empirically investigates the relationship between the grain imports competition effect and the efficiency of land for grain production from the perspective of virtual land trade based on the new-new trade theory and induced technological innovation theory. We obtained the following conclusions: (1) Overall, the efficiency of land for grain production showed a slow upward trend from 2003 to 2020, with a growth rate of 27.53%. Among them, the efficiency of land for grain production in the Huang-Huai-Hai and northeast regions has increased by 66.41% and 36.30%, respectively. (2) The grain imports competition effect reduces the profitability of domestic grain production through shocks and forces the improvement of China’s grain production land efficiency. It is caused by the trade-for-competition effect. Importing a large number of grain products with lower land use costs reduces the profits of grain producers in the domestic market through shocks, gradually eliminating producers with less efficient land use, thus forcing the improvement of land use efficiency in domestic grain production. (3) The grain imports competition effect is stronger because the developed livestock industry in the northern region requires the import of large quantities of soybeans and corn, and other virtual land content is much lower than the domestic feed grain. Also, the marginal effect of the grain imports competition effect is stronger due to the presence of more producers with less efficient land for grain production in non-major grain-producing regions, which are more likely to be eliminated by market shocks from imports. This study verifies the applicability of the trade promotion competition effect and induces the technological innovation effect in the field of grain trade, which extends the research boundary of virtual land trade in grain.
Optimized In Vitro Restriction Digestion Protocol for Preparing Maize and Barley ddRAD-Seq Libraries
Authors: Puchta-Jasińska Marta , Bolc Paulina , Piechota Urszula , Boczkowska Maja
Published: November 2023

In recent years, high-throughput sequencing methods have become increasingly popular in molecular biology laboratories, mainly due to the relatively low cost of small, benchtop platforms, the simplicity of library preparation, and the low price per unit of information. Sequencing huge and complex genomes, such as cereal genomes, remains challenging and may not always be necessary. Therefore, several techniques have been developed to sequence a reduced representation of the genome. The most flexible and widely used of these is ddRAD-Seq, which uses a pair of restriction enzymes to generate a pool of DNA fragments. The aim of this study was to validate in vitro the efficacy of different combinations of restriction enzymes for ddRAD-Seq library construction in barley and maize. Eleven pairs of restriction enzymes were selected and tested to determine the concentrations of fragments with the expected length range and to select suitable pairs for sampling the genomes of these two cereals using ddRAD-Seq. For the selected pairs, i.e., PstI—MspI and HindIII—FspBI for barley and maize, respectively, libraries were prepared for NGS sequencing on Illumina MiSeq. Sequencing confirmed the suitability of the selected enzymes to perform ddRAD-Seq in different genotypes. The results presented can be used for extensive research on these important cereal species.
Characterization of Three Sugarcane Varieties as Agro-Residue for Bioenergy Use in the Ecuadorian Andes
Authors: García-Montoya Juan , Quinteros Omar , Chimbo-Yépez Gabriel , Álvarez Luis , Velázquez-Martí Borja
Published: November 2023

There is a growing trend toward the use of renewable sources to produce clean energy and mitigate the effects of climate change. Second-generation lignocellulose biomasses, such as agro-residues, comprise a potential energy source as a byproduct of agriculture. Ecuador has optimal climate conditions that allow for the cultivation of different types of crops. This makes agriculture a relevant economic activity in the country; however, the residues obtained from agriculture have not been investigated for the establishment of a bioenergy industry. This study evaluated the potential of three varieties of sugarcane bagasse, named PR 980, CC 85-92, and CB 40-59, for bioenergy production in the Ecuadorian Andes. The bagasse was quantified by means of weighing, and then evaluated via calorimetry and thermogravimetric analysis as well as proximal and elemental analysis. The results showed that these materials met the criteria for direct combustion, exhibiting both a low nitrogen content of 0.30 ± 0.12% and ash values of 6.20 ± 1.20%. Among the analyzed varieties, CB 40-69 stood out as the most suitable for power generation within the cogeneration system; this was attributed to its superior dry calorific value of 17.37 ± 1.45 MJ kg−1, greater presence of volatile materials, and negligible ash content. Variety CB 40-69 (157.91 t ha−1) reported the highest biomass and bagasse production (56.32 t ha−1). Analysis of the SCB structure concluded that the three varieties did not differ significantly in their contents of lignin, hemicellulose, and cellulose, which is essential to implementing an industrial process for bioethanol production.
Rapid Visual Detection of African Swine Fever Virus with a CRISPR/Cas12a Lateral Flow Strip Based on Structural Protein Gene D117L
Authors: Zhang Desheng , Jiang Sen , Xia Nengwen , Zhang Youwen , Zhang Jiajia , Liu Anjing , Zhang Chenyang , Chen Nanhua , Meurens Francois , Zheng Wanglong , Zhu Jianzhong
Published: November 2023

African swine fever virus (ASFV) is a large double-stranded DNA virus that is highly infectious and seriously affects domestic pigs and wild boars. African swine fever (ASF) has caused huge economic losses to endemic countries and regions. At present, there is still a lack of effective vaccines and therapeutics. Therefore, rapid and accurate detection is essential for the prevention and control of ASF. The portable DNA endonuclease (Cas12a)-mediated lateral flow strip detection method (Cas12a-LFS) combined with recombinant polymerase amplification (RPA) has been gradually recognized as effective for virus detection including ASFV. In this study, based on the ASFV structural protein p17 gene (D117L), an RPA-Cas12a-LFS detection method was established. The detection method exhibits a sensitivity of up to two gene copies and has no cross-reaction with nine other swine viruses. Thus, the method is highly sensitive and specific. In 68 clinical samples, the coincidence rate of the p17 strip was 100%, compared to the traditional quantitative PCR (qPCR). In conclusion, we have developed a simple, rapid, sensitive, and specific ASFV visual detection method and demonstrated the potential of on-site detection of ASFV.
Applications of Romanian Propolis in Phyto-Inhibitory Activity and Antimicrobial Protection: A Comparative Study
Authors: Heghedűş-Mîndru Ramona Cristina , Glevitzky Mirel , Heghedűş-Mîndru Gabriel , Dumitrel Gabriela-Alina , Popa Maria , Popa Doriana Maria , Radulov Isidora , Vică Mihaela Laura
Published: November 2023

Propolis use in medicine, pharmaceutical, cosmetic, and food industries is well known. This study aimed to investigate propolis’ phyto-inhibitory and antimicrobial potential. Nine propolis samples obtained from distinct Romanian regions and characterized in terms of physical–chemical parameters, phenols and flavonoid contents, and antioxidant properties were prepared as dry propolis and aqueous extracts. The phyto-inhibitory effect was comparatively tested on different cereals: hexaploid bread wheat (Triticum aestivum), maize (Zea mays L.), oats (Avena sativa L.), and barley (Hordeum vulgare L.), while their in vitro antimicrobial activity was evaluated against bacterial and fungal strains specific to cereals: Bacillus subtilis, B. cereus, Proteus mirabilis, Fusarium oxysporum, Penicillium chrysogenum, and Aspergillus niger. All propolis samples showed a phyto-inhibitory effect on the cereals, the most pronounced being corn and oats. Propolis powder samples displayed a lower phyto-inhibitory activity than propolis extracts. Also, all tested products showed inhibitory efficacy against both bacteria and fungi. Furthermore, principal component analysis showed differences between the samples’ phyto-inhibitory and antimicrobial properties depending on the geographical origin. Positive correlations were found between the polyphenols, flavonoid content, and antioxidant activity, respectively. These data support propolis’ phyto-pharmaceutical potential related to its use in plant crop management as an alternative in ecological agriculture.
Contribution of Mitochondrial Reactive Oxygen Species to Chronic Hypoxia-Induced Pulmonary Hypertension
Authors: Yan Simin , Sheak Joshua R. , Walker Benjimen R. , Jernigan Nikki L. , Resta Thomas C.
Published: November 2023

Pulmonary hypertension (PH) resulting from chronic hypoxia (CH) occurs in patients with chronic obstructive pulmonary diseases, sleep apnea, and restrictive lung diseases, as well as in residents at high altitude. Previous studies from our group and others demonstrate a detrimental role of reactive oxygen species (ROS) in the pathogenesis of CH-induced PH, although the subcellular sources of ROS are not fully understood. We hypothesized that mitochondria-derived ROS (mtROS) contribute to enhanced vasoconstrictor reactivity and PH following CH. To test the hypothesis, we exposed rats to 4 weeks of hypobaric hypoxia (PB ≈ 380 mmHg), with control rats housed in ambient air (PB ≈ 630 mmHg). Chronic oral administration of the mitochondria-targeted antioxidant MitoQ attenuated CH-induced decreases in pulmonary artery (PA) acceleration time, increases in right ventricular systolic pressure, right ventricular hypertrophy, and pulmonary arterial remodeling. In addition, endothelium-intact PAs from CH rats exhibited a significantly greater basal tone compared to those from control animals, as was eliminated via MitoQ. CH also augmented the basal tone in endothelium-disrupted PAs, a response associated with increased mtROS production in primary PA smooth muscle cells (PASMCs) from CH rats. However, we further uncovered an effect of NO synthase inhibition with Nω–nitro-L-arginine (L-NNA) to unmask a potent endothelial vasoconstrictor influence that accentuates mtROS-dependent vasoconstriction following CH. This basal tone augmentation in the presence of L-NNA disappeared following combined endothelin A and B receptor blockade with BQ123 and BQ788. The effects of using CH to augment vasoconstriction and PASMC mtROS production in exogenous endothelin 1 (ET-1) were similarly prevented by MitoQ. We conclude that mtROS participate in the development of CH-induced PH. Furthermore, mtROS signaling in PASMCs is centrally involved in enhanced pulmonary arterial constriction following CH, a response potentiated by endogenous ET-1.
The NADPH Oxidase Inhibitor, Mitoapocynin, Mitigates DFP-Induced Reactive Astrogliosis in a Rat Model of Organophosphate Neurotoxicity
Authors: Meyer Christina , Grego Elizabeth , S. Vasanthi Suraj , Rao Nikhil S. , Massey Nyzil , Holtkamp Claire , Huss Joselyn , Showman Lucas , Narasimhan Balaji , Thippeswamy Thimmasettappa
Published: November 2023

NADPH oxidase (NOX) is a primary mediator of superoxides, which promote oxidative stress, neurodegeneration, and neuroinflammation after diisopropylfluorophosphate (DFP) intoxication. Although orally administered mitoapocynin (MPO, 10 mg/kg), a mitochondrial-targeted NOX inhibitor, reduced oxidative stress and proinflammatory cytokines in the periphery, its efficacy in the brain regions of DFP-exposed rats was limited. In this study, we encapsulated MPO in polyanhydride nanoparticles (NPs) based on 1,6-bis(p-carboxyphenoxy) hexane (CPH) and sebacic anhydride (SA) for enhanced drug delivery to the brain and compared with a high oral dose of MPO (30 mg/kg). NOX2 (GP91phox) regulation and microglial (IBA1) morphology were analyzed to determine the efficacy of MPO-NP vs. MPO-oral in an 8-day study in the rat DFP model. Compared to the control, DFP-exposed animals exhibited significant upregulation of NOX2 and a reduced length and number of microglial processes, indicative of reactive microglia. Neither MPO treatment attenuated the DFP effect. Neurodegeneration (FJB+NeuN) was significantly greater in DFP-exposed groups regardless of treatment. Interestingly, neuronal loss in DFP+MPO-treated animals was not significantly different from the control. MPO-oral rescued inhibitory neuronal loss in the CA1 region of the hippocampus. Notably, MPO-NP and MPO-oral significantly reduced astrogliosis (absolute GFAP counts) and reactive gliosis (C3+GFAP). An analysis of inwardly rectifying potassium channels (Kir4.1) in astroglia revealed a significant reduction in the brain regions of the DFP+VEH group, but MPO had no effect. Overall, both NP-encapsulated and orally administered MPO had similar effects. Our findings demonstrate that MPO effectively mitigates DFP-induced reactive astrogliosis in several key brain regions and protects neurons in CA1, which may have long-term beneficial effects on spontaneous seizures and behavioral comorbidities. Long-term telemetry and behavioral studies and a different dosing regimen of MPO are required to understand its therapeutic potential.
Hydrogen: A Rising Star in Gas Medicine as a Mitochondria-Targeting Nutrient via Activating Keap1-Nrf2 Antioxidant System
Authors: Cheng Danyu , Long Jiangang , Zhao Lin , Liu Jiankang
Published: November 2023

The gas molecules O2, NO, H2S, CO, and CH4, have been increasingly used for medical purposes. Other than these gas molecules, H2 is the smallest diatomic molecule in nature and has become a rising star in gas medicine in the past few decades. As a non-toxic and easily accessible gas, H2 has shown preventive and therapeutic effects on various diseases of the respiratory, cardiovascular, central nervous system, and other systems, but the mechanisms are still unclear and even controversial, especially the mechanism of H2 as a selective radical scavenger. Mitochondria are the main organelles regulating energy metabolism in living organisms as well as the main organelle of reactive oxygen species’ generation and targeting. We propose that the protective role of H2 may be mainly dependent on its unique ability to penetrate every aspect of cells to regulate mitochondrial homeostasis by activating the Keap1-Nrf2 phase II antioxidant system rather than its direct free radical scavenging activity. In this review, we summarize the protective effects and focus on the mechanism of H2 as a mitochondria-targeting nutrient by activating the Keap1-Nrf2 system in different disease models. In addition, we wish to provide a more rational theoretical support for the medical applications of hydrogen.
Frequency and Antimicrobial Resistance Patterns of Foodborne Pathogens in Ready-to-Eat Foods: An Evolving Public Health Challenge
Authors: Abalkhail Adil
Published: November 2023

Food products that are ready-to-eat have become increasingly popular in recent years due to their efficiency, affordability, and convenience. However, there are concerns about public health because certain products, particularly animal products, may contain antibiotic-resistant bacteria. This study aimed to quickly and accurately identify foodborne pathogens, such as Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), in samples of shawarma and chicken burgers using peptide mass fingerprinting (PMF) technology. Additionally, the prevalence and levels of antibiotic resistance in the pathogens were determined. The study utilized 300 samples obtained from fast food restaurants in Al Qassim, Saudi Arabia. A variety of methods were used to identify foodborne pathogens, including culture on specific media, bacterial counts by numerical dilutions of homogenized samples, and proteome identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The Kirby–Bauer method was applied to detect the susceptibility and resistance of the bacteria to various antibiotics. PCR was utilized to identify antimicrobial resistance genes such as blaTEM, tet(A), blaZ, and mecA in S. aureus and E. coli isolates. The percentage of E. coli, S. aureus, Salmonella, Listeria monocytogenes (L. monocytogenes), Acinetobacter baumannii (A. baumannii), and Hafnia alevei (H. alevei) was 34%, 31%, 10.67%, 7.33%, 6.67%, and 4%, respectively. Shawarma samples were found to contain the highest levels of pathogens, compared with chicken burger samples. According to the MBT Compass Flex Series Version 1.3 software, all isolates were identified with 100% accuracy. The log score for MBT identification ranged from 2.00 to 2.56. Among E. coli isolates, ampicillin, and penicillin had the highest resistance rate (100%), followed by tetracycline (35.29%). A number of antibiotics were reported to be resistant to S. aureus, including nalidixic acid (100%), followed by penicillin (96.77%), piperacillin (45.16%), and norfloxacin (32.26%). Some E. coli isolates were susceptible to tetracycline (49.02%), nalidixic acid (47.06%), and piperacillin (43.14%), whereas amikacin was the only drug that was effective against 32.72% of S. aureus isolates. The proportions of the blaTEM and tet(A) genes in E. coli isolates were 55.89% and 45.1%, respectively, whereas S. aureus strains did not possess either of these genes. However, 21.5% and 47.31% of blaz and mecA genes were present among various isolates of S. aureus, respectively. In contrast, E. coli strains did not possess either of these genes. In conclusion, the fast identification and antimicrobial profiles of the foodborne pathogens were useful in identifying which restaurants and fast food outlets may need to improve their food safety practices. Ultimately, our results will be used to devise targeted strategies to control foodborne pathogens.
Evaluation of the Rock Mechanical Properties of Shale Oil Reservoirs: A Case Study of Permian Lucaogou Formation in the Jimusar Sag, Junggar Basin
Authors: Xiong Jian , Gan Renzhong , Liu Xiangjun , Liang Lixi , Guo Xiucheng
Published: November 2023

Rock mechanical properties play an important role in the exploration and development of shale oil reservoirs. To study the rock mechanical properties continuously distributed along the longitudinal direction of the formation, physical and mechanical property data of shales from the Permian Lucaogou Formation of the Junggar Basin were gathered through experimental tests. The regression analysis method was applied to obtain relationships between physical properties and rock mechanical properties. Based on this, new empirical relationships between rock mechanical properties were established. The results show that the uniaxial compressive strength (UCS) ranged from 48.40 to 147.86 MPa, the Young’s modulus (Es) was between 3.02 and 20.63 GPa, the Poisson’s ratio (νs) ranged from 0.13 to 0.36, the cohesive force (C) ranged from 14.65 to 34.60 MPa, and the internal friction angle (φ) was between 27.61 and 46.94°. The rock mechanical properties were more sensitive to the P-wave interval transit time (Δtc) and bulk density (DEN). Among them, the UCS was more sensitive to Δtc, while the C, Es, and νs were more sensitive to Δtc/DEN. For UCS and Es, an exponential function correlation is more reliable than linear expression and power function, whereas for C and νs, power function and linear expression were adopted for higher accuracy, respectively. Compared with the empirical equations presented in the literature, the empirical equations established in the paper are more accurate and reliable, making them applicable to the Permian Lucaogou Formation shale oil reservoirs in the Jimusar Sag of the Junggar Basin.
Defining the URCOTEBS System as a Unilateral Radiographic–Stochastic Model for the Complementary States (Health/Disease) of the D-Organ and Middle-Ear Mucosa
Authors: Rădulescu Marian , Mocanu Adela-Ioana , Neagu Alexandra-Cristina , Schipor Mihai-Adrian , Mocanu Horia
Published: November 2023

The middle ear (ME) is a notoriously complicated anatomic structure, geometrically arranged as irregular interlinked spheroidal and polyhedric cavities dug inside the temporal bone (TB). The bony walls of these cavities are radiopaque and form the bony support for the D-Organ that we have previously defined as corresponding to the epithelium covering the Antrum walls (belonging to the central cavities of the middle ear) and the walls of mastoid and petrous cavities (the peripheral cavities of the ME). The aim of the study is to define an exact method for categorizing a Unilateral Radiographic COnformation of the TEmporal Bone in Schuller’s projection (URCOTEBS) under one of the four defined conformations and using it for practical everyday clinical purposes. The conclusion is that a radiograph in Schuller’s projection is a concrete way of storing precise information on the status (health/disease) of the D-Organ and therefore of the ME mucosa. These data is encoded within the image and we aim to decode and translate them into clinical data. The URCOTEBS results in an overlapping projection of all bony cavities that comprise the General Endo-temporal Bony Cavity Complex onto the same plain (film). This characteristic of classical film imaging constitutes an advantage from the multiple CT sections, as far as our proposed approach goes, because the set of stochastic information is found in the whole of the cavities taken as one on the same image, to which the measurement gauges can be easily applied. The decoding must be performed accordingly, and this occurs much faster with conventional radiography. This image of the TB in Schuller’s projection is a mirror that reflects the status of the ME mucosa, and URCOTEBS encodes the physiological state of the D-Organ. The present work gives, through stochastic methods, the key to decoding this information into clinical language. In ascending order of their projection areas (projection of their Variable Geometry Peripheral Endo-temporal Bony Cavity Complex) we can recognize URCOTEBS_d, URCOTEBS_c, URCOTEBS_b, and URCOTEBS_a. The corresponding Greek letter designates the state of disease for each of these conformations: URCOTEBS_δ, URCOTEBS_γ, URCOTEBS_β, URCOTEBS_α, and the capital letters define their state of health: URCOTEBS_D, URCOTEBS_C, URCOTEBS_B, URCOTEBS_A. URCOTEBS_d is the smallest unilateral radiographic conformation of the TB in Schuller’s projection and is, by definition, a radiographic image of the state of disease of the D-Organ. The probability of disease in URCOTEBS_d is 100%. This radiographic system is readily available and clinically usable.
Analysis of Nature-Based Solutions Research Trends and Integrated Means of Implementation in Climate Change
Authors: Choi Eunho , Kim Raehyun , Chae Jeongyeon , Yang A-Ram , Jang Eunjo , Lee Ki Yong
Published: November 2023

Nature-based solutions (NbS) is an approach to solving climate change and social issues based on nature. Despite NbS being widely studied as an effective method to solve social problems, the trends in NbS research have hardly been analyzed. Therefore, this study examined change patterns in NbS-related research topics over time and analyzed the interactions of NbS research and relevant activities in various fields. After reviewing research papers based on the search term ‘nature-based solutions’ on Scopus, and collecting 1567 research papers, we conducted dynamic topic modeling (DTM) and network analysis. The papers were classified into 19 topics via DTM. Water, forest, and urban topics made up the greatest portion of NbS research, while NbS topics in the forest sector showed a steady increase over time. This study also found close connections between NbS studies on forests and other sectors and confirmed that the forest sector can become an integrated means of contributing to climate change responses and other resultant social issues. This study demonstrates that DTM and network analysis are useful tools for understanding the trends in NbS research and finding the linkages between various fields.
Fractional Steps Scheme to Approximate the Phase Field Transition System Endowed with Inhomogeneous/Homogeneous Cauchy-Neumann/Neumann Boundary Conditions
Authors: Fetecau  Constantin , Moroşanu Costică , Stoicescu Dorin-Cătălin
Published: November 2023

Here, we consider the phase field transition system (a nonlinear system of parabolic type) introduced by Caginalp to distinguish between the phases of the material that are involved in the solidification process. We start by investigating the solvability of such boundary value problems in the class Wp1,2(Q)×Wν1,2(Q). One proves the existence, the regularity, and the uniqueness of solutions, in the presence of the cubic nonlinearity type. On the basis of the convergence of an iterative scheme of the fractional steps type, a conceptual numerical algorithm, alg-frac_sec-ord-varphi_PHT, is elaborated in order to approximate the solution of the nonlinear parabolic problem. The advantage of such an approach is that the new method simplifies the numerical computations due to its decoupling feature. An example of the numerical implementation of the principal step in the conceptual algorithm is also reported. Some conclusions are given are also given as new directions to extend the results and methods presented in the present paper.
The Review of Existing Strategies of End-of-Life Graphite Anode Processing Using 3Rs Approach: Recovery, Recycle, Reuse
Authors: Kosenko Alexandra , Pushnitsa Konstantin , Pavlovskii Alexander A. , Novikov Pavel , Popovich Anatoliy A.
Published: November 2023

While past recycling efforts have primarily concentrated on extracting valuable metals from discarded cathode materials, the focus is now shifting towards anode materials, particularly graphite, which makes up 10–20% of LIB mass. Escalating prices of battery-grade graphite and environmental considerations surrounding its production highlight the significance of graphite recycling. This review categorizes methods for graphite recovery into three approaches: recovery, recycle, and reuse. Moreover, it explores their potential applications and comparative electrochemical performance analysis, shedding light on the promising prospects of utilizing spent graphite-based functional materials. The review underscores the importance of sustainable recycling practices to address the environmental and economic challenges posed by the proliferation of LIBs and the growing demand for graphite.
Influence of Hydrodynamic Forces on Electroactive Bacterial Adhesion in Microbial Fuel Cell Anodes
Authors: Godain Alexiane , Vogel Timothy M. , Fongarland Pascal , Haddour Naoufel
Published: November 2023

This investigation examined the role of shear stress on the dynamic development of microbial communities within anodic biofilms in single-chamber microbial fuel cells (MFCs). Bacterial attachment to surfaces, often regarded as a crucial step in biofilm formation, may significantly contribute to the selection of electroactive bacteria (EAB). It is well established that hydrodynamic forces, particularly shear forces, have a profound influence on bacterial adhesion. This study postulates that shear stress could select EAB on the anode during the adhesion phase by detaching non-EAB. To examine this hypothesis, MFC reactors equipped with a shear stress chamber were constructed, creating specific shear stress on the anode. The progression of adhesion under various shear stress conditions (1, 10, and 50 mPa) was compared with a control MFC lacking shear stress. The structure of the microbial community was assessed using 16S rRNA gene (rrs) sequencing, and the percentage of biofilm coverage was analyzed using fluorescence microscopy. The results indicate a significant impact of shear stress on the relative abundance of specific EAB, such as Geobacter, which was higher (up to 30%) under high shear stress than under low shear stress (1%). Furthermore, it was noted that shear stress decreased the percentage of biofilm coverage on the anodic surface, suggesting that the increase in the relative abundance of specific EAB occurs through the detachment of other bacteria. These results offer insights into bacterial competition during biofilm formation and propose that shear stress could be utilized to select specific EAB to enhance the electroactivity of anodic biofilms. However, additional investigations are warranted to further explore the effects of shear stress on mature biofilms.
Heterologous Production of Isopropanol Using Metabolically Engineered Acetobacterium woodii Strains
Authors: Höfele Franziska , Schoch Teresa , Oberlies Catarina , Dürre Peter
Published: November 2023

The depletion of fossil fuel resources and the CO2 emissions coupled with petroleum-based industrial processes present a relevant issue for the whole of society. An alternative to the fossil-based production of chemicals is microbial fermentation using acetogens. Acetogenic bacteria are able to metabolize CO or CO2 (+H2) via the Wood–Ljungdahl pathway. As isopropanol is widely used in a variety of industrial branches, it is advantageous to find a fossil-independent production process. In this study, Acetobacterium woodii was employed to produce isopropanol via plasmid-based expression of the enzymes thiolase A, CoA-transferase, acetoacetate decarboxylase and secondary alcohol dehydrogenase. An examination of the enzymes originating from different organisms led to a maximum isopropanol production of 5.64 ± 1.08 mM using CO2 + H2 as the carbon and energy source. To this end, the genes thlA (encoding thiolase A) and ctfA/ctfB (encoding CoA-transferase) of Clostridium scatologenes, adc (encoding acetoacetate decarboxylase) originating from C. acetobutylicum and sadH (encoding secondary alcohol dehydrogenase) of C. beijerinckii DSM 6423 were employed. Since bottlenecks in the isopropanol production pathway are known, optimization of the strain was investigated, resulting in a 2.5-fold increase in isopropanol concentration.