bluuebunny/crossref_metadata_2025 huggingface dataset:
Hypermethylation of the microRNA miR-124, miR-125b, miR-127, and miR-129 in ovarian carcinoma is involved in suppression of their expression and associated with both the development and progression of ovarian cancer
Authors: Braga E. A. , Pronina I. V. , Utkin D. O. , Filippova E. A. , Burdennyy A. M. , Loginov V. I. , Fridman M. V. , Kazubskaya T. P. , Kushlinskii N. E.
Published: February 2019

Rationale: We have previously identified a group of microRNA genes (MIR-107, MIR-1258, MIR-130b, MIR-34b/c, MIR-9-1, MIR-9-3 et al.), whose methylation was involved into the development and progression of ovarian cancer. Aim: To expand the range of microRNA genes hypermethylated in ovarian cancer and to study the role of this modification in the pathogenesis and progression of ovarian cancer. Materials and methods: The study was performed on a series of 76 ovarian cancer and 13 peritoneal metastases samples. The method of bisulfite DNA conversion followed by methylation-specific polymerase chain reaction (PCR) was used to assess the methylation status of the microRNA genes; the expression of these genes was measured by quantitative real-time PCR. Results: Compared to histologically unchanged ovarian tissue, there was a significant increase in methylation frequencies in the tumor samples for 6 microRNA genes studied: MIR-124-1, MIR-124-2, MIR-124-3, MIR-125B-1, MIR-127, and MIR-129-2 (p ≤ 10-3). The expression level of 4 microRNAs (miR-124-3p, miR-125b-5p, miR-127-5p, miR-129-5p) encoded by these genes was suppressed, with a significant correlation between changes in their expression levels and the gene methylation (rs = 0.63–0.94, p ≤ 10-4). In addition, there were statistically significant associations between methylation of 5 genes (MIR-124-2, MIR-124-3, MIR-125B-1, MIR-127, and MIR-129-2) and the parameters of cancer progression, such as its clinical stage, metastatic spread, tumor size and invasion, and to a lesser extent with a decrease in the differentiation grade. The association of 5 microRNA genes with metastatic spread was confirmed by the analysis of peritoneal macro-metastases from 13 patients. Conclusion: We have demonstrated the functional significance of aberrant methylation in a group of microRNA genes for suppression of their expression in ovarian carcinomas. There is an association of microRNA gene hypermethylation with the progression of ovarian cancer, including metastatic spread to the peritoneum.
Dendrokronološka analiza debelinskega priraščanja smreke (Picea abies (L.) Karst.) na območju njene naravne in umetne razširjenosti v Sloveniji
Authors: Stopar Samo , Jevšenak Jernej , Kobler Andrej , Levanič Tom
Published: February 2019

A dendrochronological analysis of the spruce's radial increment at eight different plots was conducted throughout Slovenia. The plots selected were diverse: five of them were natural spruce stands; in others, the spruce was introduced anthropogenically in the past for economic reasons, or its natural share in stands was increased. We took samples of at least 20 trees per plot and examined them in the dendrochronological laboratory using Advanced Tree-Ring Image Capturing System (ATRICS) and specialized dendrochronological software. We compared the growth in natural spruce stands with the secondary (anthropogenic) ones and found no statistical differences in growth patterns between these two groups. However, we found some evidence for climate change effect on radial increment at locations Sorško polje, Ravnik and Mašun, which indicates that air temperature and precipitation now have stronger effect on spruce growth than in the past. Analysis of pointer years suggests that extreme events (exceptionally warm and dry years) are more common and widespread in the last 30 years.
Les pratiques déclarées de l’enseignement de l’oral au primaire. Qu’en est-il en Belgique francophone ?
Authors: Colognesi Stéphane , Deschepper Catherine
Published: February 2019

Cette contribution apporte des éléments de réponses aux recherches sur les pratiques déclarées des enseignants menées dans différents territoires francophones. Elle a en effet pour visée de traiter les questions suivantes : Que font les enseignants belges pour travailler les compétences en Savoir Parler et Écouter ? Quelles sont les pratiques et les gestes professionnels qu’ils estiment mobiliser dans leurs pratiques ? À quelle fréquence et pourquoi travaillent-ils l’oral en classe ? C’est l’analyse des données recueillies par questionnaire auprès de 290 enseignants du primaire qui nous permet d’élaborer des éléments de réponse.
Extraction and Evaluation of Linseed Mucilage as Binding Agent in Prednisolone Tablet 20 mg
Authors: Alaa Balla Suliman Abuelrakha  , Elnazeer I. Hamedelniel
Published: February 2019

Great interest been have develop to discover new excipients to solve problem that faced us during formulation like incompatibility and compressibility that lead to slow down manufacturing processes. Linseed mucilage has properties that suggested it as useful additives and binding agent like inertness, nontoxic and viscosity. The purpose of the present study was to extract and evaluate linseed mucilage as binding agent in prednisolone tablets. The Linseed mucilage was extracted and evaluated for physicochemical properties using official procedures. Tablet was prepared by wet granulation. Granules evaluation revealed satisfactory results. Three formula were prepared that contain three different percent from linseed mucilage 3%, 5% and 7%.The hardness test result show great increase in tablet hardness 4.23, 5.59 and 7.76, respectively. The dissolution test was carried in Ph 1.2 for 2 hours and cumulative drug release 20.33%, 17.88% and 14.49% respectively .And at Ph 6.8 for 4 hours cumulative drug release 78.18%, 75.43% and 69.19%. The tablets formulated showed acceptable general appearance. Formula mucilage 7% showed the best flowbility of granules, hardness and friability when compared with formulas 3% and 5%.In weight variation test indicated that the type and concentration of mucilage used were not significant. When increase the percent of mucilage in formula combined with increase in tablet hardness. Invitro dissolution study indicated that the main factors which influence the amount of drug released were the pH of the medium used and concentration of the mucilage.
Interactive multimedia library products: forming skills of technology while preparing bachelors of library and information activities
Authors: Savkina S. V.
Published: February 2019

Currently, the range of information products and services provided remotely is developing and expanding. Modern library users often prefer remote services to traditional forms. The real communication is inferior to virtual one, the popularity of electronic information products is growing. Libraries transform popular traditional products and services into electronic formats. So traditional quizzes, which are popular in libraries, are moving into a new form, become a dynamically developing electronic product. The popularity and demand of multimedia products for modern users led to emerging a wide range of such products in the library practice. Multimedia capabilities combine different forms of information and provide visibility, visual appeal and increase interest among users. The most original of them are the products with interactive features that provide the opportunity to dialogue with users and implement active actions. The most popular are such interactive multimedia products as an electronic interactive exhibition, a multimedia interactive quiz, a video with interactive elements. Libraries can also develop such products without extensive software, hardware and human resources. To ensure the quality of the created interactive multimedia products, it is necessary to study the technology of their preparation. Based on the interactive multimedia works created by students the author considers their features, cases of interactive opportunities realization; characterizes the experience to form of skills of technology of their preparation at bachelors of library and information activity. Actively developing of remote library services using information-communicating technologies, transforming traditional forms into multimedia format contributes to the development of interactive multimedia forms, as well as allows attracting users and increase the demand for documents. Game forms of work with readers are especially relevant for children and youth audience. Development and implementtation of multimedia interactive product is economically less labor-intensive process than the traditional library event.
Book in the literary discourse of the German Enlightenment
Authors: Misyurov N. N.
Published: February 2019

The books’ role in the German Enlightenment discourse explores the intersection of different but complementary disciplines: bibliology, philosophy of culture and «text linguistics», as well as the history of literature that expands the possibilities of studying cultural and public communication. A text (magazine, book) is interpreted as a mechanism that controls the process of learning and understanding. To illustrate the study theoretical foundations, the author considers the historical practice of the literary era in Germany in 1770-1790. The struggle of «cilturtregers» to renew German culture moved from the political sphere to journalism and literature. The author concludes that the book (both scientific and artistic) and reading became a factor of social communication. The struggle for the renewal of German culture, its national identity preservation due to a number of historical reasons hindering the development of the country moved from the political sphere to the journalism and literature field. It was closely connected with the whole complex of the European Enlightenment ideas. The book - both scientific (philosophical work, art treatise) and artistic one (literary and journalistic composition, dramatic creation, etc.) became an indispensable tool of the nation aesthetic education. In such circumstances, a book obtains the significance of not only the «source of knowledge» but a kind of «catechism» to struggle for national culture. Thus, considering a book text as a phenomenon of the German culture of the Enlightenment century with ideological and aesthetic significance, it should be especially notes that such a «text» (a book of scientific, philosophical, moralistic or artistic content) is addressed both to a specific reader, a representative of some class, and to a «collective reader». The German novel (it is the genre of «trivial» literature that is considered directly) is a product of the era. The dialogue «author - reader» (or a complicated triad «author - publisher - reader») was the basis of the nation estetic education. Reading became a fact (and a factor) of social communication. The German book has been transformed from an expensive and exclusive «source of knowledge» targeted for scientists, connoisseurs of «beauty» to a catechism (available for the ordinary reader) of the national struggle to preserve the German culture self-existence and to acquaint the nation to the treasures of the world classical «ancient» and modern literature.
Complex information resources of a research library: modernization and development
Authors: Yudina I. G. , Bazyleva E. A.
Published: February 2019

Library scientists have been actively considering the ways to improve various aspects of library activities recent decades. The most studied problems are the following: libraries innovative development in general, information systems updating, e-resources and services modernization, etc. The paper analyzes the history of creating the most significant complex information resources generated by the Branch of the State Public Scientific Technological Library of the Siberian Branch of the Russian Academy of Sciences. It shows that both internal (human, technological) and external (reform of the Russian Academy of Sciences, emerging new software products or platforms) factors can evidently be reasons for upgrading some specific electronic products. Modernization of the library information resources can follow several areas: structure development, content updating, design improvement, changing generation technologies, hard- and software update, providing services based on resources, carrying out promotion activities. Using the e-resources property for continuous transformation, it’s possible to increase their life cycle significantly. At the same time, the main goal of modernizing library information resources is to improve their quality. The library information resources updating is a natural process in some extend permitting to improve content and service components of a resource for its providing in a modern form to a wide range of users. The choice of the resource improvement direction depends on its relevance, and developers’ competence level.
Services demand of scientific and technical libraries: professionals’ opinion
Authors: Paramonova I. E.
Published: February 2019

The problems of demand is extremely significant for scientific and technical libraries (STL). The libraries within organizations depend directly on the support of people who make decisions. Their value for the enterprise development is no longer as obvious for senior management. There is a temptation to attribute STL’ low status not only to the «unfriendly» information policy by the management, but to admit that this problem is communicatory in a greater degree. The demand for any library is closely related to the establishing the connection with stakeholders. To generate user and non-user awareness, and raise conversance on available services and resources, STL need to find ways to promote services and resources. In the services marketing, promotion is any communicative activity which object is forming and stimulating interests to a product, organization or activity. STL need to choose the right vector of their activity, to reveal «bottleneck», overcoming which the library is able to take on. The paper represents preliminary results of a survey of STLs’ employees to identify the most perspective services (from the point of respondents’ view). It concludes that the majority of respondents noted the insufficient demand for library services, which is reflected in decrease in number of visits and printed publications demands. In this regard, STLs try to create digital collections and introduce modern services according to their technical equipment. Modern specialist lives under the time deficiency conditions. As a result, there is a lack of time to read including professional literature. Therefore, as a popular service is the preparation of thematic collections and digests. According to some respondents, «library staff should be ready to compile texts on request». In general, this is quite consistent with the global trend, the manifestation of which is the growing popularity of projects such as «Short Reading» and «Smart reading». Respondents indicated the relevance of such areas as intellectual property management. At the same time, they noted the insufficient level of existing competencies. STLs should be a guide of information culture for their users. The library is quite capable to occupy this niche, acting as a navigator, consultant and even coach. A similar experience already exists.
RISK ANALYZE OF WORK AMONG THE WIG WORKER IN PURBALINGGA
Authors: Ulfah Nur , Harwanti Siti , Ngadiman Ngadiman
Published: February 2019

PURPOSE : to investigate the factors associated with myopia disease. The aim in particular to know the relationship between age, years of service, lighting intensity, fatigue, nutritional status with myopia disease.DESIGN : product moment correlationMETHODS ; data were analyzed from 85 participans. To identify the prevalence of myopia disease by measuring the sharpness of the eye using a snellen card. Then the prevalence of myopia is associated with factors of age, work period, light intensity, fatigue and nutritional statusRESULTS : showed that the variables associated with myopia disease are age, years of service, and the intensity of illumination (each value of p = 0.000) <0.05. While the variables that are not associated with myopia disease is the fatigue (p = 0.193> 0.05) and nutritional status (p = 0.594> 0.05).CONCLUSIONS : for the company is necessary to add lighting to meet minimum standards in the workplace section Knitting, necessity of providing health insurance for health care of eyes, especially the elderly for the efficiency of his eyesight did not continue to decline should be occur rotation system of labor have which has been decreased visual  acuity of the knitting to another section. Keyword  : Risk analyze, work, worker
The Consumption of Fresh Vegetables from Street Food and Sanitation of Street Stalls at Four Locations in Bogor City
Authors: Utami Donna Fujie Rahaditha , Rahayu Winiati P , Nuraida Lilis
Published: February 2019

The consumption of fresh vegetables at the stalls needs serious attention. This research aimed to estimate the exposure probability due to fresh vegetables consumption of street food consumers, to measure sanitation level of street stalls that serving fresh vegetables, and to recommend a mentoring program for the stalls at four locations in Bogor City. This research was conducted at 16 stalls located at four locations in Bogor City. The number of respondents surveyed was 293 people and determined by stratified sampling method. Food frequency questionnaire was used as a tool in the survey. The survey showed that men consumed more fresh vegetables than women with the average of 47.12 g/person/consumption and frequency 3.37 times/week (p<0.05). Adults consumed fresh vegetables at the stalls most often with 3.05 times/week and the average consumption of 44.59 g/person/consumption (p<0.05). All street stalls were at level IV of sanitary practices. Thus, there is a risk of food safety in consuming fresh vegetables at the stalls at four locations in Bogor City. Men and adults had high exposure probability to microbiological hazards due to fresh vegetables consumption at the stalls. Mentoring program for all locations involving socialization and implementation of street stall management and sanitation practices regulations by government, full awareness of street vendors in complying with the prevailing regulations, and firm action for the vendors who violate the rules.
Impacto de los costos de calidad en la ejecución de los proyectos de construcción en Colombia
Authors: Silva Giraldo César Augusto , Dugarte Mendoza Juan Sebastián , Mejía Jálabe Aizar
Published: February 2019

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 El impacto que genera el plan de gestión de calidad de un proyecto se ve reflejado en indicadores de medición elementales para la toma de decisiones. Es tal su importancia dentro del progreso de cualquier proyecto, que si se ejecuta de forma adecuada puede evitar la inclusión de valores adicionales y posibles reprocesos.

Por ello, el presente artículo tiene por objetivo analizar el impacto de los costos de calidad en la ejecución de proyectos de construcción en Colombia. A través de una investigación de tipo de correlacional con enfoque cuantitativo, se desarrolló un estudio de caso a partir de ocho proyectos de construcción de edificaciones, que llevó a concluir que no es evidente la recuperación de los costos de calidad, en ninguna de las opciones analizadas, bajo los métodos de simulación de Montecarlo y el análisis del valor ganado, por lo cual los costos deben ser presupuestados y tenidos en cuenta en las diversas etapas de ejecución de los proyectos.
Diagnóstico de prácticas de iniciación y planeación en gerencia de proyectos en pymes del sector de la construcción
Authors: Giraldo González Germán Eduardo , Castañeda Mondragón Juan Carlos , Correa Basto Orlando , Sánchez Ángel Juan Carlos
Published: February 2019

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El presente artículo presenta los resultados del diagnóstico de las prácticas de iniciación y planificación en gerencia de proyectos para pymes del sector de la construcción en Bogotá, Colombia. Esta investigación se basó en el estudio de la bibliografía internacional relacionada con las pymes, la gerencia de proyectos en el sector de la construcción y los procesos de iniciación y planificación. La información y los hallazgos se compararon con un conjunto de entrevistas y encuestas realizadas a gerentes de proyectos con experiencia relevante en la materia, lo que dio como resultado una caracterización de las prácticas en gerencia de proyectos que se aplican actualmente en las pymes del sector de la construcción. La descripción se realizó mediante la división de los hallazgos en las áreas de conocimiento establecidas en la guía del PMBOK ® y la extensión para proyectos de construcción, para finalmente determinar las variables que pueden usarse en un futuro modelo para la gerencia de proyectos en pymes.
Factores críticos de éxito para la implementación de Business Process Management (BPM): estudio de caso para la cadena de suministro de una empresa del sector floricultor
Authors: Gutiérrez Sánchez Alexander , Rodríguez Ríos Claudia , Santos Hernández Andrés Felipe
Published: February 2019

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Se diseñó un modelo que muestra la interacción entre los factores críticos de éxito (FCE) para implementar Business Process Management (BPM) y los procesos de la cadena de suministro a través de cada una de las fases de implementación, se identificaron aquellos FCE más relevantes a través de un análisis de la literatura y se validaron por un grupo de expertos. Luego se utilizó un enfoque de estudio de caso en un cultivo de flores ubicado en la Sabana de Bogotá para validar empíricamente el modelo propuesto. Los resultados se convirtieron en un diagnóstico que guía a esta empresa sobre los aspectos prioritarios para mejorar y poder dirigir sus esfuerzos hacia estos en particular, asegurando un mayor grado de éxito en la implementación de BPM en este proceso.El modelo establecido propone un marco de referencia para futuras investigaciones que permitan hacer un diagnóstico y determinar el nivel en el que se encuentre la empresa de acuerdo con los FCE propuestos.
Dinámica de sistemas: una forma de optimizar la gestión del riesgo
Authors: Del Río Cortina Abel Aníbal , Cárdenas Quintero Beitmantt Geovanni
Published: February 2019

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Este documento presenta un modelo de gestión de riesgos de proyectos de investigación, estableciendo un acercamiento integral al manejo del riesgo en el marco de un sistema complejo, generando una aproximación a la gestión de riesgos mediante la aplicación de dinámica de Sistemas, con el objetivo de mitigar las limitaciones de las metodologías tradicionales como PMBOK, PRINCE2, e ISO31000, con respecto a una perspectiva de análisis estructural.La propuesta tiene como punto de partida las consideraciones de la gestión del riesgo plasmadas en las metodologías tradicionales, resaltando sus puntos fuertes y haciendo énfasis en las limitaciones de carácter estructural; posteriormente se desarrolla un análisis de la importancia del tratamiento de los riesgos en los proyectos de investigación y se cuestiona el distanciamiento que presentan este tipo de proyectos con respecto a metodologías de gestión validadas. Finalmente se aborda el contexto del Instituto de Gestión del Riesgo (IRM) y se procede a la propuesta de modelo.
El rol de la cooperación en la propensión para innovar: evidencia de la encuesta chilena de innovación
Authors: Chávez Bustamante Felipe
Published: February 2019

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La innovación y la cooperación han estado estrechamente relacionadas como tópicos principales en la literatura reciente sobre management. La perspectiva de innovación abierta ha tomado en cuenta la importancia de capitalizar las ganancias de la cooperación en los esfuerzos para innovar, sin embargo, hay una falta de investigación empírica de este fenómeno para las economías emergentes.Esta investigación tuvo por objetivo analizar el efecto de la cooperación en la propensión a innovar de las firmas, utilizando datos de la Encuesta Chilena de Innovación. Se encontró que la cooperación tiene un efecto positivo, estadísticamente significativo, en la propensión a innovar. Las implicancias del trabajo están relacionadas con la implementación de políticas, ya que según lo observado, el paradigma debiese desplazarse hacia un entorno entrelazado y estratégico de negocios, que haga más plausible la participación de las firmas en esfuerzos de redes colaborativas.
5 acciones para la toma de decisión en investigación, Desarrollo e innovación I+D+i
Authors: Rodríguez-Rojas Yuber Liliana , Luque-Clavijo Ana María , Rodríguez-González Liliana Yamile , Riveros-Clavijo Julián Andrés
Published: February 2019

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La gestión de la investigación académica y empresarial incluye la identificación de líneas de investigación que direccionen los fondos y los esfuerzos técnicos de grupos que se dedican a actividades de Investigación, Desarrollo e innovación (I+D+i). En el proceso de toma de decisión que orienta esta gestión, suele tener prevalencia la opinión derivada de las experiencias e intereses individuales de los investigadores por encima de la consulta objetiva de diversas fuentes que pueden proporcionar información que optimice dicho proceso.El objetivo de esta investigación fue la validación de una metodología que vincula las dos perspectivas para la toma de decisión en I+D+i, dando a conocer resultados parciales de 5 acciones propuestas para la identificación y fortalecimiento de líneas de investigación de grupos de investigación académicos y empresariales.Dicha validación demostró la pertinencia, claridad y aplicabilidad de las acciones propuestas, lo que las convierte en herramientas útiles para la toma de decisión en investigación, tecnología e innovación y el establecimiento de líneas de investigación.
¿Qué tan competitivos son los países miembros de la Alianza del Pacífico en infraestructura de transporte?
Authors: Cortés Villafradez Raúl Alberto
Published: February 2019

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Este documento analiza por medio de un estudio descriptivo, cómo el desarrollo de infraestructura fue un elemento importante de la competitividad, durante el periodo 2006-2016.En esta investigación se compara el comportamiento de la inversión en infraestructura de transporte frente a variables que miden los resultados de la competitividad de la misma, como lo son: costos, tiempo, calidad y desempeño logístico para cada uno de los países miembros de la Alianza del Pacifico, basado en fuentes de información tales como el Banco Mundial, World Economic Forum (WEF), Economic Commission for Latin America and the Caribbean (ECLAC) e Infralatam.A partir de dicho análisis se pudo evidenciar la estrecha relación existente entre la calidad de la infraestructura de transporte y el nivel de competitividad de los países estudiados.
Neurodiseño: aplicación de la neurotecnología a la interpretación de las emociones que afectan la decisión de compra online en plataformas e-commerce del sector retail español
Authors: Puente Berdasco Daniel , Pinilla Díaz Carlos Andrés
Published: February 2019

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Entender la esencia psicofisiológica de las emociones se ha convertido en una necesidad imperante en el desarrollo de las estrategias del marketing actual, debido al gran protagonismo que el cliente ha logrado frente a los tradicionales métodos comerciales, donde productos y/o servicios eran el eje principal de actuación. Esta investigación tiene como objetivo identificar e interpretar, a través del uso de herramientas descriptivas y neurocientíficas, el tipo de emociones que experimentan los usuarios al realizar procesos de compra online y cómo estas pueden influir en su decisión final. Se demostró, según pruebas realizadas, que factores emocionales y de ergonomía web —diseño, usabilidad, seguridad— influyen directamente en la decisión de compra del consumidor
Measuring Treatment Fidelity: From Research to Implementation
Authors: Martindale-Adams Jennifer , Zuber Jeffrey , Graney Marshall J. , Burns Robert , Nichols Linda Olivia
Published: February 2019

Background: Treatment fidelity is commonly assessed in research trials because greater fidelity helps later replication in practice and is generally associated with better outcomes. However, implementations after initial research may not be as faithful to the original protocol, leading to unsuccessful replication.
Methods: Fidelity methods of the Resources for Enhancing All Caregivers Health in the VA (REACH VA) dementia caregiving clinical implementation were examined with comparisons to those of the Resources for Enhancing Alzheimer’s Caregivers Health (REACH II) randomized clinical trial. The NIH treatment fidelity conceptual framework was used, which includes design, provider training, delivery, receipt, and enactment. To translate these concepts into practice, for each REACH VA session, core activities that measured delivery, receipt, and enactment were identified. These core activities were then examined for a sample of 293 caregivers of veterans with dementia who received the REACH intervention from June 2012 to September 2014.
Results: Methods to assess fidelity were not as extensive for REACH VA, compared to REACH II. However, fidelity methods were present in all five framework aspects. Overall completion of core items for delivery and receipt/enactment was 92%. Completion of core items declined as sessions continued, with delivery declining from 97.2% of items completed for Session 1 to 85.7% for Session 4; receipt/enactment declined from 97.3% to 88.4%.
Conclusions: Consistent attention to maintaining treatment fidelity may explain why caregiver outcomes are similar in both the original REACH II randomized clinical trial and in the REACH VA implementation. The process followed to assess REACH fidelity is applicable to other evidence-based interventions that have been or are going to be implemented into practice. Fidelity assessment need not be continually re-invented, nor must it be elaborate. Critical components of an intervention as developed and practiced can and should be used to assess fidelity seamlessly. The interplay between how an intervention fits into the organization, ongoing assessment of its practitioners, and its effect on those participating in it should be seamless. In this way, fidelity assessment is not an add-on or burdensome for busy providers, supervisors, and organizations.
Trial Registration: ClinicalTrials.gov: NCT00177489, Registered 12 September 2005, retrospectively registered.
Keywords: Treatment fidelity; translational research, reproducibility; reliability; validity.
A Tree of Life? Multivariate logistic outcome-prediction in disorders of consciousness
Authors: Steppacher Inga , Fuchs Peter , Kaps Michael , Nussbeck Fridtjof , Kissler Johanna
Published: February 2019

Background: Currently, the clinical outcome of patients with very severe head injuries and subsequent chronic disorders of consciousness is seen as generally very poor. Here, we specify individual outcome chances for disorders of consciousness (DOC) patients on the basis of clinical and EEG data and identify different subgroups of DOC patients, who vary substantially regarding their outcome chances.
Methods: We employed data from 102 patients with DOC and used standard clinical protocol data (age, etiology, diagnosis, gender) and early sensory (N100, Mismatch Negativity) and late cognitive (P300, N400) EEG event related potentials (ERPs) to predict patients recovery rates.
Results: Two significant prediction models emerged: In both, subgroups of patients with good (51%, tree 1) to very good recovery chances (97%, tree 2) could be identified. The first model was obtained from standard clinical data. The second model included late cognitive ERPs (N400 and P300) and resulted in considerably better patient classification. Moreover, when taking cognitive event related potentials into account, the standard protocol data did not add further significant information, neither did early ERPs (N100, MMN). This highlights that cognitive ERPs provide decisive information about an individual patient’s prognosis.
Conclusion: The presented information about outcome chances of individual disorders of consciousness patients will be vital for these patients and critical for clinical professionals who have to direct specialized treatments and council relatives. Legal guardians and families, in turn, need to know what to expect in the future in order to prepare for the challenges ahead.
Keywords: Disorders of consciousness, Unresponsive wakefulness syndrome, Minimal consciousness state, Logistic outcome-prediction.
Sevoflurane regulating LncRNA Rik-203 contributes to neural differentiation via microRNA-466l-3p /BDNF pathway
Authors: Zhang Lei , Liu Qidong , Xue Zhenyu , Jia Yan , Jiang Hong
Published: February 2019

Background: The anesthetics inhibit neural differentiation, induced neuron loss and cognitive impairment in young animals. However, the underlying mechanisms of anesthesia on neural differentiation and are unknown.
Methods: Embryonic stem cells (ESCs) and mice received sevoflurane anesthesia. RNA sequencing; gene expression of mRNAs, LncRNAs and miRNAs; over-expression and RNA interference of genes; flow cytometry; real-time quantity PCR and Western blot were used in the studies. RNA pull-down assay and PCR were employed to detect any miRNA that attached to Rik-203. The binding of miRNA with mRNA of BDNF was presented by the luciferase assay.
Results: Here we found that LncRNA Rik-203 was higher expressed in mice brain than other tissues and increased during neural differentiation. Sevoflurane decreased the level of Rik-203 in mice brain. Knockdown of Rik-203 repressed the neural differentiation derived from mouse embryonic stem cell with the downregulation of the neural progenitor cells markers Sox1 and Nestin. RNA pull-down showed that miR-466l-3p was highly bound to Rik-203 and mediated the function of Rik-203. Inhibition of miR-466l-3p restored the neural differentiation repressed by Rik-203 knockdown. BDNF was directly targeted by miR-466l-3p and also downregulated by sevoflurane. Overexpression of BDNF restored the neural differentiation repressed by miR-466l-3p and Rik-203 knockdown.
Conclusion: Our study suggested that sevoflurane related LncRNARik-203 facilitates neural differentiation by inhibiting miR-466l-3p’s ability to reduce BDNF levels.
Keywords: Anesthesia; sevoflurane; Rik-203; miR-466l-3p; BDNF; neural differentiation.
Efficacy of Fungicide Treatments for Control of Anthracnose Canker in Young Cider Apple Trees in Western Washington
Authors: Garton Whitney J. , Mazzola Mark , Alexander Travis R. , Miles Carol A.
Published: February 2019

Anthracnose canker, caused by Neofabraea malicorticis, threatens the sustainability of cider apple (Malus ×domestica) production in the maritime climate of western Washington. In the short-term, the disease reduces overall orchard productivity and in the long-term it reduces an orchard’s economic life span. The disease is difficult to manage using cultural practices, and information on fungicide efficacy is limited and contradictory. To address this situation, a 2-year study was conducted to evaluate efficacy of zinc (4.49 lb/acre), basic copper sulfate (2.49 lb/acre), captan (2.94 lb/acre), thiophanate-methyl (0.69 lb/acre), pyraclostrobin plus boscalid (0.38 lb/acre), and combinations of these fungicides to manage anthracnose canker infection in young cider apple trees cultivated in a maritime climate. Trees used in the first year of the study (2016) were found to be infected by anthracnose canker on receipt, so the first year was a measure of disease control and the second year (2017) was a measure of disease prevention. In 2016, when fungicide treatments were applied every 3 weeks from March through October, none of the treatments evaluated inhibited the development of new infections or the expansion of existing cankers (77% increase in canker size on average for all treatments). In 2017, when fungicide treatments were applied every 3 weeks from February through April, two to three new cankers were observed 3 weeks after final treatment application for all treatments. Results from this study demonstrate that the current fungicides recommended for control of anthracnose canker are not reliably effective in the orchard environment of northwest Washington. Future studies should assess the fungicides evaluated in this study applied in rotation with additional systemic fungicides.
EVOLUTION OF THE BACKGROUND SYSTEM OF INTEGRATION OF DOMESTIC ENTERPRISES INTO THE WORLD ECONOMY: THEORETICAL PROBLEMS
Authors: Mazaraki Anatolii , Lagutin Vasyl , Lositska Tetyana
Published: February 2019

Modern civilization processes are due to production processes. In any management system, the enterprise is its key element. The theoretical definitions of the essence of “production” and “enterprise” concepts in certain aspects do not coincide with the corresponding legal definitions. This clearly illustrates the dynamics of domestic legislation at the stages of the adoption of relevant laws of Ukraine from the “Law on Enterprises” to the Economic Code of Ukraine. Prospects for the integration of domestic enterprises into international production networks are significantly dependent on their ability to move to modern management standards, systematically coordinated with sustainable development processes based on innovatization, focused on the requirements of entry into the information society space. In Ukraine, during the period of state independence, the adoption as a national series of key international standards is implemented. But the unevenness of the evolution of the components of both constant and technological development is also determined. This gives rise to corresponding negative consequences and requires a search for mechanisms by which the characteristics of the harmony of the above components will be determined, on the basis of which it will be possible to effectively manage them in order to bring them to the desired state.
TRANSFORMATION OF STRUCTURAL COMPONENTS OF MASTICATORY MUSCLES OF IMMATURE ANIMALS IN DIFFERENT STAGES OF MERCAZOLILUM-INDUCED HYPOTHYROIDISM
Authors: Sahan Nazar
Published: February 2019

The aim of the study. The aim of this study was to establish the features of the structural organization of the chewing and lateral pterygoid muscles of immature rats during the developmental stages of experimental hypothyroidism.

Materials and methods. The material for the study was the chewing and lateral pterygoid muscles of 48 white mongrel immature rats-males. The following research methods were used: mercazole-induced hypothyroidism modelling, injection method for studying the bloodstream, histological examination of blood vessels and tissue elements, electron microscopy, morphometric analysis, biochemical methods, statistical evaluation of the results.

Results. A violation of thyroid homeostasis (a decrease in the concentration of thyroid hormones in the blood) has been established. In the bloodstream, edematous changes are observed histologically and submicroscopically. A decrease in the number of hemocapillaries per unit area and the number of hemocapillaries per muscle fiber have been established morphometrically. In muscle fibers, cross striation with vacuolization of endomysia and perimisia is lost. Edematous changes are observed, which are aggravated depending on the duration of experimental hypothyroidism. The glycolytic muscle fibers are more affected, and the changes are aggravated depending on the duration of the experiment. It has also been found out that there is a redistribution in the amount of muscle fibers. There is a tendency to a decrease in oxidative-glycolytic and a significant increase in glycolytic muscle fibers.

The morphological changes that have been found out are accompanied by violations of the macroelement balance, in particular, the content of Ca, P, Mg, which determine the functioning of skeletal muscle tissue.

Conclusion. Thus, at different periods of hypothyroidism, edematous changes occur both in the vascular wall and in muscle fibers, which are aggravated depending on the duration of hypothyroidism and are caused by a decrease in the basic metabolic rate, slowing of redox processes and tissue hypoxia. At the same time, chromotropic substances are released from bonds with proteins and accumulate in the intermediate substance with the subsequent replacement of collagen fibers by mucus-like masses.
DIAGNOSTIC VALUE OF MORPHOLOGICAL CHANGES IN GASTROESOPHAGEAL REFLUX DISEASE IN BIOPSY MATERIAL OF THE DISTAL ESOPHAGUS IN ADOLESCENTS SICKLY WITH ACUTE RESPIRATORY DISEASES
Authors: Zhuravel Olena , Pochinok Tetyana , Zadorozhna Tamara , Archakova Tetyana , Zamula Valentyna
Published: February 2019

The article dedicated to the problem of the diagnostic value of morphological changes in gastroesophageal reflux disease in the biopsy of the distal esophagus in pubertal children of childbearing age.

Aim of the research is to investigate the diagnostic value of morphological changes in gastroesophageal reflux disease in esophageal biopsy material in adolescents sickly with acute respiratory diseases.

Methodology. The objective of the study was achieved through examination of 90 adolescents (10 to 16 years old, average age 13.1±3.54 years) kept under observation at the Children’s Clinical Hospital No. 9 of Kyiv and on the basis of the Department of Pediatrics No. 1 Center of Primary Health Care No. 4 of the Desnianskyi district of Kyiv. All adolescents belonged to the group of sickly with a number of respiratory diseases averaging 6-8 times a year, lasting from 8 to 18 days (on average 12.8±5.41 days). All children have undergone endoscopic examination of the esophagus, stomach and duodenum with the esophagus mucosa biopsy using the OLYMPUS GIF-P3 flexible fiberscope.

Results. It was found that the least valuable diagnostic feature in the morphological examination of the mucous membrane of the distal esophagus in the pain-causing children with GERD was thickening of the epithelium with a sensitivity of 13,0 %, a specificity of 96.0 %, and total value of 65.0 %. It has been proved that hyperplasia of cells of the basal layer of the mucous membrane of the distal esophagus at the GERD in the infected children is 46.7 % (specificity – 93.3 %, the total value is 75.6 %). Increase in the number of papillae and their prolongation in 33.3 % cases (sensitivity – 33.3 %, specificity – 93.3 %, overall diagnostic value – 70.8 %).

Conclusion. The peculiarity of the morphological manifestations of GERD in childbearing children is dystrophic changes in keratocytes in the superficial parts of the multilayer squamous epithelium, which are detected at 100.0 % of patients (specificity is 93.3 %, total value is 96.8 %), with parakeratosis centers at 13.3 % of cases. It has been shown that a frequent and diagnostically valuable indication is inflammatory infiltration of the esophageal mucosa, which are verified in all cases (100.0 %, with dilatation and hyperemia in 46.7 % of patients (specificity – 40.0 %, total value – 81.3 %).
PROBATION OF THE APPARATUS FOR LOW-TEMPERATE PROCESSING OF MEAT CULINARY PRODUCTS BY IR-RADIATION
Authors: Zahorulko Andrii , Zagorulko Aleksey , Yancheva Maryna , Serik Maksym , Sabadash Sergei , Savchenko-Pererva Marina
Published: February 2019

The improvement of existent technological processes of making meat culinary products and development of the correspondent principally new resource-effective equipment is an urgent task. There was probated the developed apparatus of the low-temperature processing of meat culinary products by IR-radiation with the further qualitative assessment of ready culinary products for solving it.

The results of the conducted studies provide making high-quality, juicy meat products without a crust that generally provides spreading the assortment of products under conditions of their low-temperature processing in a price category, attractive for a consumer.

A flexible film resistive electric heater of the radiating type (FFREhRT) was used as a heating element for providing the even low-temperature processing of meat products at the expanse of taking the working geometry of a chamber of the apparatus by it. For providing the autonomy of the work of air-ejecting ventilators, there was offered to use Peltier elements that allow to transform secondary heat of FFREhRT in the low-voltage feeding tension and to provide cooling of the external surface of the apparatus synchronously. The apparatus is mobile that provides its displacement directly in places of realization. At the studies, there was established the culinary readiness degree of meat products of the spheric form with diameter 0,1±0,01m by reaching temperature 53…85 оС in the product center.

The evenness of the heat flow distribution within the working chamber was proved by the data, obtained from thermocouples, placed on the metal spheric model of a meat product sample in real time by 7 thermocouples.

As a result of analyzing the geometric model of placing Peltier elements, it has been established, that for providing the low-voltage tension of ventilators at level 3,55 V, 10 successively connected elements are necessary.

The offered innovative construction-technological solutions allow to make high-quality, juicy meat products without a crust that generally provides spreading the assortment of products under conditions of their low-temperature processing.
INVESTIGATION OF THE INFLUENCE OF CRYOPOWDERS “BROCCOLI” AND “LAMINARIA” ON QUALITY PARAMETERS OF CHEESE MASSES OF DIFFERENT FAT
Authors: Hachak Yuriy , Slyvka Natalya , Gutyj Bogdan , Vavrysevych Jaroslava , Sobolev Alexander , Bushueva Inna , Samura Tatyana , Paladiychuk Olena , Savchuk Liubov , Pikhtirova Alina
Published: February 2019

Crafty combination of cryopowders as bio-supplements to the “milk” base has great prospects in both bio-technological and social aspect. Main criteria at developing recipes of cheese masses were a search for optimal ratios of components for getting proper normative taste characteristics. In this connection, there is offered to study the influence of cryopowders “Broccoli” and “Laminaria” on quality parameters of cheese masses of the new generation, namely ones of the treating-prophylactic destination.

The analysis of organoleptic characteristics of salty cheese masses with the cryopowders “Laminaria” and “Broccoli” demonstrates that they have undergone no essential changes and correspond to normative requirements. Thus, the color of salt masses with the cryopowder “Laminaria” was light-grey with a green tint: at using the cryopowder “Broccoli” – grey with a yellow tint. The smell of cheese masses remained fresh, sour-milk. But the expressed smell of the added cryosupplement was felt in samples. The smack of experimental samples at adding the cryopowder “Broccoli” was more expressed. Their consistence was homogenous, delicate, paste-like.

The titrated acidity of experimental samples of salty cheese masses with the cryopowder “Laminaria” was 124–130 0Т, mass share of moisture 62–60 % and mass share of dry substance – 40–38 %, and values of energetic value were 164 and 118 kcal/100 g of the product. The titrated acidity of experimental samples of salty cheese masses with the cryopowder “Broccoli” was 126–134 0Т, mass share of moisture 63–66 % and mass share of dry substances – 34–37 %, and values of energetic value were 174 and 128 kcal/100 g of the product.

Cheese salty masses with using the cryopowders “Laminaria” and “Broccoli” as phytosupplements are products of the increased biological value, which production doesn’t need the additional specialized equipment, is a potential source as to widening the assortment of products of the treating-prophylactic destination.