bluuebunny/crossref_metadata_2025 huggingface dataset:
A Study on the Characteristics of Exhibition Contents of Women's Museum
Authors:   The Korean Society of Culture and Convergence, Heo Bokyung , Lee Min
Published: June 2023

Today, as non-mainstream stories appear as exhibition contents in history museums, it can be seen that there is a need for research on the subject and expression method. Therefore, with the goal of providing basic data on women's museums as a preliminary study, the probability of the original data and expression were examined, focusing on the exhibition of women's museums. Chapter 1 raises the background of the study and the need for the aforementioned preliminary investigation, and Chapter 2 examines the meaning and characteristics of the women's museum. In Chapter 3, the basic status, exhibition system, direction, and operation direction of each institution were investigated for the Bonn Women’s Museum in Germany, Gender Museum of Denmark, and Merano Women’s Museum in Italy. As a result of the study, it was found that the composition context that began with the grassroots movement affects the direction of operation. Second, as it expanded to the issue of “gender and society” rather than “women versus men” the scope of women's museum acceptance also expanded, which seems to affect the expression of exhibition contents. Third, we found the need to review women's things. Finally, all three institutions had something in common that they used the historicity of space to richly convey women's stories and create values by discovering historicity and meaning on their own.
The Effect of College Students' Growth Mindset on Protean Career Attitude: The Dual Mediating Effect of Career Identity and Proactive Personality
Authors:   The Korean Society of Culture and Convergence, Cho ji yeon
Published: June 2023

The purpose of this study is to confirm the effect of college students' growth mindset on their protean career attitude, and to verify the dual mediating effect of career identity and proactive personality in the relationship between them. A survey was conducted on 219 college students. For the collected data, descriptive statistics and Pearson's correlation analysis were conducted with SPSS 25.0. To verify the mediating effect, the PROCESS macro's Model 6 was used to confirm the dual mediating effect of career identity and proactive personality and the significance of indirect effects was verified using the bootstrapping method. The main results are as follows. First, the growth mindset of college students showed a positive effect on the protean career attitude. Second, the mediating effect of proactive personality was verified in the relationship between college students' growth mindset and protean career attitude. Third, in the relationship between college students' growth mindset and protean career attitude, there was a dual mediating effect of career identity and proactive personality. Based on these conclusions, the theoretical and practical implications and limitations of the study were discussed.
The Uncertainty of Volunteering System in the Leprosarium Sorokdo
Authors:   The Korean Society of Culture and Convergence, Goo Youngsan
Published: June 2023

The purpose of this study is to explore the characteristics of volunteer activities in the medical field, focusing on the case of Sorokdo National Hospital. Based on the participatory observation of Sorok Island, which has been intermittently conducted from May 2006 to the present, the aspects of volunteer activities in Sorok Island were examined. ‘Sorokdo Volunteer Service’ was historically created in response to patients’ lack of health and lack of relationship. In addition, the current volunteer system also tends to be institutionalized patient-centered in response to this deficiency. However, in the process, it could be seen that volunteers are exposed to various crisis situations that escape the control of the current service system. By analyzing the service experience of volunteers continuously performed in this crisis situation, the dailiness of the volunteer service system in Sorok Island was raised as a problem. There are definitely virtues of the medical field where volunteering is routine based on the participation of many different subjects. However, it was argued that the fact that volunteers are repeatedly placed in crisis situations outside the control of the system in the medical field, leaning on their familiar daily life, increases the uncertainty of the service system.
Analysis of Determinants of Customer Satisfaction on Design Cut’s Hair Style
Authors:   The Korean Society of Culture and Convergence, Lee Seojeong , Kwon Ki Han
Published: June 2023

This study aimed to analyze customer satisfaction with design haircut services and identify the factors that determine it through empirical analysis. Among the three factors of reliability, image, and involvement, the study found that reliability and image had a significant impact on customer satisfaction. Firstly, reliability in design haircut had a close association with customer satisfaction (t=7.020, p<.001). This is because the higher the customer's trust in hairstyling services, the higher their satisfaction. Secondly, the hypothesis that hairstyling and image can influence personal self-esteem and customer satisfaction had a positive effect (t=6.422, p<.001). This is because it can help improve not only self-confidence in appearance but also social or relationship images. Thirdly, involvement did not have a significant impact on customer satisfaction, which may be because customers focus on the design and service itself rather than interactions with designers or staff in design haircut services. Therefore, this study confirmed that reliability and image in design haircut services are related to customer satisfaction, and focusing on improving these factors can increase customer satisfaction.
A Study on the Quality Management Method of University Education through the Class Improvement Plan (CQI) Perception Survey: Centering on S Universities
Authors:   The Korean Society of Culture and Convergence, Shin Geunyeong
Published: June 2023

The purpose of this study is to explore the structure and related guidelines and regulations of the CQI, which is being implemented for quality management of education at S University, and to investigate the perception of professors on the CQI. Based on the results, it provides practical help in managing the quality of education. The subjects of the study were 129 professors working at S University, and a questionnaire was constructed after reviewing theories and previous studies. The research questions were, first, to explore the guidelines and regulations related to CQI, second, to analyze the current status of CQI by college and professor position at S University in the first semester of the 2022 academic year, and third, to analyze the differences between the results of the questionnaire and professor characteristics, resulting in the following research findings. It was confirmed that various measures to induce active participation, such as excellent lecture workshops and reflection of teacher performance evaluations, which are used as external motivations to increase professors' participation rates, are needed.
Proposal of Digital Carbon Journey Map
Authors:   The Korean Society of Culture and Convergence, Yang Na Yeong , Pan Younghwan , Yoo Chaemoon
Published: June 2023

The Korean government and companies are adopting a digital-based approach to achieve carbon neutrality by transforming their industrial structure (i.e. digital transformation). However, as the demand for contactless online services increases, so does the level of network and data usage, leading to a rapid rise in overall carbon emissions within the service environment. To mitigate these emissions, it is essential to incorporate an environmental perspective into the service provision environment. Given the limited amount of existing scholarly research on the environmental impacts of service improvement tools, this study proposes a toolkit for visualizing carbon emissions associated with customer usage. Specifically, a 'digital carbon journey map' is proposed, which integrates carbon emissions into the existing 'customer journey map'. This proposed framework enables a comprehensive analysis of services, taking into account both environmental considerations and customer satisfaction. Its objective is to provide a guidance for future eco-friendly service design and implementation.
INCONSISTENCY OF PHARMACEUTICAL PERSONNEL LICENSE REGULATION IN GOVERNMENT REGULATION NUMBER 51 YEAR 2009 CONCERNING PHARMACEUTICAL WORK
Authors: Kisma Said Ismala
Published: June 2023

The issuance of Government Regulation Number 51 of 2009 concerning Pharmaceutical Work has the aim of encouraging social change to become a better direction through legal certainty. This normative legal research method examines the law from an internal perspective with the object of research being legal norms. The purpose of this research is to analyze the regulation of pharmacy staff licenses in Government Regulation No. 51 of 2009 concerning Pharmaceutical Work and to analyze the ideal setting of pharmaceutical staff in positive law. The results of the study explain that the regulation of permits for pharmaceutical workers in Government Regulation Number 51 of 2009 concerning Pharmaceutical Work can refer to Article 1 paragraph 22 or 52 paragraph (2) letter a of Government Regulation Number 51 of 2009 concerning Pharmaceutical Work. There is an inconsistency in the arrangement due to the content of the material being regulated contradicting each other. Ideally the Regulation of Pharmaceutical Workers in Positive Law, that Government Regulation Number 51 of 2009 concerning Pharmaceutical Work, nomenclature is not in accordance with the nomenclature stipulated in Law Number 36 of 2009 concerning Health, especially between the phrase work and practice.
QUO VADIS: LEGAL CERTAINTY OF INFORMAL WORKER THROUGH MANPOWER ACT
Authors: Prasetyo Wicaksono Muhammad , Rusdjijati Retno , Kurniati Bachtiar Nia , Abdul Hakim Hary , Bagus Edhita Praja Chrisna
Published: June 2023

Issue related to legal protection policies for informal sector workers in Indonesia still leaves a lot of debate among our society. The basis of this employment is in Law Number 13 of 2003 concerning Manpower which is further perfected and becomes a new hope for workers in Indonesia, this hope is stated in Law Number 11 of 2020 concerning Job Creation. However, the regulation needs to comprehensively regulate the existence of informal sector workers in Indonesia. Informal workers received no guarantees or protection; many had sum-proportional wages, were found to be physically ophysically abused, and had low welfare. This study aims to analyze legal protections for informal sector workers in Indonesia. This research is normative legal research that uses a legal and conceptual approach. The data used is secondary data consisting of primary, secondary, and tertiary legal materials. Data collection techniques in this study were carried out with a review literature study and then analyzed with a qualitative descriptive method. This study's results show that a legal vacuum (rechtsvacuum) regulates the existence of informal sector workers in Indonesia. It is inherent that informal sector workers receive more attention from the government as they are the largest group of workers in Indonesia. If analyzed with 'triadism law' theory, the current legal regulations in Indonesia are considered not to have fulfilled the elements of the purpose of law itself. Therefore, with that in mind to ensure the rights of informal sector workers, it is necessary to have clear rules and guarantee workers' rights including health insurance guarantees, legal umbrellas, environmental empowerment, welfare, and social protection.
THE REVIEW OF ISLAMIC CRIMINAL LAW TOWARD CRIMINAL ACTION OF A SHARP WEAPON MISUSE
Authors: AZ Mutiara
Published: June 2023

Carrying a sharp weapon is a criminal act which can cause harm to other people, both on property and even killing other people. In addition to the consequences, carrying a sharp weapon can also cause harm to the criminal himself. The sharp weapon misuse is actually not a new thing. It is a form of crime as stipulated in Article 2 Paragraph 1 of the Emergency Decree Number 12 year 1951. The objectives of this study were to find out how was the basis for judges' considerations in imposing criminal sanctions and the review of  Islamic criminal law in adjudication  of District Court of Palembang Number 821/Pid.Sus/2020/PN.Plg. The method used was the Normative legal research method, which was obtained from literature review (Library Research). The results of the study showed that before imposing a criminal decree, the judge first payed attention and considered the basic principles of justice, legal certainty, utility in imposing a sentence based on the crime committed by the defendant.
REINSURANCE ARRANGEMENTS ASSOCIATED WITH GUARANTEE LEGAL RESPONSIBILITIES
Authors: Santri Selvi Harvia
Published: June 2023

The use of diversion responsibility or reinsurance arrangements as a way for insurance companies to protect themselves from losses by cooperating with a reinsurance company. One reason for utilizing reinsurance is to distribute risk. Reinsurance becomes more understandable when insurance claims occur, and it is not possible to have reinsurance without having insurance first. The legal framework for insurance and reinsurance is explained in the Criminal Code or Law Number 40 of 2014 concerning business insurance. However, there is no provision that clearly defines what is meant by reinsurance, which can cause problems if there are insufficient answers regarding the company's reinsurance payment for losses. Therefore, legal certainty is necessary. The research objective is to determine the form of responsibility of the guarantor in reinsurance, and the normative law method is used. The Ex-Gratia Payment clause outlines the responsibility of the reinsurance company to the insured, allowing the insured to claim compensation from the insurer for reinsurance claims.
Role of CEO Power and Foreign Ownership in the Selection of Public Accounting Firms and the Effectiveness of the Audit Committee as Moderators
Authors: Nursaman Dede , Harnovinsah  , Nurmala Ahmar
Published: June 2023

The purpose of this study is to analyze the role of CEO power and foreign ownership of the election a Public Accounting Firm (KAP) and the effectiveness of the Audit Committee as a moderator. The research objects used are companies that are included in the IDX80 index on the Indonesian Stock Exchange 2017-2021. Based on the purposive sampling technique,  sample of 42 companies was obtained which were considered to meet the criteria as research objects. The analytical method used in this study is Structural Equation Modeling (SEM) with SmartPLS 3 software. The results show partially that the higher the CEO power, foreign ownership and foreign ownership moderated by the effectiveness of the Audit Committee, the company more likely choose Big 4 KAP as external auditors. Meanwhile, the higher the CEO power, which is moderated by the effectiveness of of the Audit Committee, more likely choose non-Big 4 KAP.
Trichoderma asperellum suppresses viral diseases and promotes the growth and yield of country bean
Authors: Ali Hossain Muhammed , Tanjum Swarna Fariha , Al Arabi Rabeya , Hamim Islam
Published: June 2023

Viral diseases are the main adversaries of country bean (Lablab purpureus Lin.) production in Bangladesh. Potyviruses and cucumber mosaic virus (CMV) have been reported in country bean leaves that displayed virus-like symptoms. This study looked at the growth and yield of country bean plants that had been treated with Trichoderma asperellum to control country bean viruses. T. asperellum-treated plants exhibited decreased disease incidence up to 91% and a drop in the vector population up to 96%, when compared to control plants. Plant growth was enhanced in soil drenched with T. asperellum suspension, with an increase in the number of leaves per plant, pods per plant, root length, weight of dried pods/plant, and weight of dried seeds/plant. Finally, our findings suggest that T. asperellum could be an effective treatment for controlling viral diseases of the country bean in Bangladesh.
Age-related decrease in functional mobility score when performing a locomotor task in an immersive environment
Authors: Renaux Alexandre , Clanché Fabien , Muhla Frédéric , Duclos Karine , Meyer Philippe , Colnat-Coulbois Sophie , Gauchard Gérome
Published: June 2023

In recent years, immersive virtual reality technology has emerged in the field of health. Its use could allow the assessment of the motor behavior of individuals in adaptable and reproducible immersive environments, simulating real situations. This study aimed to assess the effect of an immersive scenario on functional mobility during a simple locomotor task according to age. Sixty young adults and 60 older volunteers, who were autonomous and without cognitive and neurological impairment participated. A locomotor task based on the “Timed Up and Go” task was performed in real and virtual conditions. A functional mobility score was calculated by combining the time and the number of steps used and compared between young and older people. Results showed that correlations between time and the number of steps were the same in VR and real conditions, but the locomotor performance decreased significantly in VR for both populations. Additionally, older people exhibited a more reduced locomotor performance in a virtual environment than young adults, thereby their functional mobility score decreased more to complete the task, reflecting the adoption of a more secure locomotion strategy often related to the fear of falling, with an increase in time and number of steps to support balance. The major difference between reality and VR is the visual immersion with an HMD, and visual information is more important in the sensory integration of older people. Therefore, the reduction in visual field and lack of visual exproprioceptive information about the body segments in the virtual environment could explain these results. Finally, the effect of immersion in a virtual scenario on mobility exists for both populations but is accentuated by the aging process and is therefore age dependent.
Reproducibility and FAIR principles: the case of a segment polarity network model
Authors: Mendes Pedro
Published: June 2023

The issue of reproducibility of computational models and the related FAIR principles (findable, accessible, interoperable, and reusable) are examined in a specific test case. I analyze a computational model of the segment polarity network in Drosophila embryos published in 2000. Despite the high number of citations to this publication, 23 years later the model is barely accessible, and consequently not interoperable. Following the text of the original publication allowed successfully encoding the model for the open source software COPASI. Subsequently saving the model in the SBML format allowed it to be reused in other open source software packages. Submission of this SBML encoding of the model to the BioModels database enables its findability and accessibility. This demonstrates how the FAIR principles can be successfully enabled by using open source software, widely adopted standards, and public repositories, facilitating reproducibility and reuse of computational cell biology models that will outlive the specific software used.
Regulation of gut microbiota: a novel pretreatment for complications in patients who have undergone kidney transplantation
Authors: Ye Jiajia , Yao Junxia , He Fangfang , Sun Jing , Zhao Zheng , Wang Yumei
Published: June 2023

Kidney transplantation is an effective method to improve the condition of patients with end-stage renal disease. The gut microbiota significantly affects the immune system and can be used as an influencing factor to change the prognoses of patients who have undergone kidney transplantation. Recipients after kidney transplantation showed a lower abundance of Firmicutes and Faecalibacterium prausnitzii and a higher proportion of Bacteroidetes and Proteobacteria. After using prebiotics, synbiotics, and fecal microbiota transplantation to regulate the microbial community, the prognoses of patients who underwent kidney transplantation evidently improved. We aimed to determine the relationship between gut microbiota and various postoperative complications inpatients who have undergone kidney transplantation in recent years and to explore how gut microecology affects post-transplant complications. An in-depth understanding of the specific functions of gut microbiota and identification of the actual pathogenic flora during complications in patients undergoing kidney transplantation can help physicians develop strategies to restore the normal intestinal microbiome of transplant patients to maximize their survival and improve their quality of life.
Comprehensive analysis of gut microbiome and host transcriptome in chickens after Eimeria tenella infection
Authors: Yu Hailiang , Wang Qi , Tang Jianqiang , Dong Liyue , Dai Guojun , Zhang Tao , Zhang Genxi , Xie Kaizhou , Wang Hongsheng , Zhao Zhenhua
Published: June 2023

Background

Coccidiosis is an intestinal parasitic disease caused by Eimeria protozoa, which endangers the health and growth of animals, and causes huge economic losses to the poultry industry worldwide every year. Studies have shown that poultry gut microbiota plays an important role in preventing the colonization of pathogens and maintaining the health of the host. Coccidia infection also affects host gene expression. However, the underlying potential relationship between gut microbiome and host transcriptome during E. tenella infection in chickens remain unclear.

Methods

In this study, metagenomic and transcriptome sequencing were applied to identify microbiota and genes in cecal contents and cecal tissues of infected (JS) and control (JC) chickens on day 4.5 postinfection (pi), respectively.

Results

First, microbial sequencing results of cecal contents showed that the abundance of Lactobacillus, Roseburia sp. and Faecalibacterium sp decreased significantly after E. tenella infection (P < 0.05), while the abundance of Alistipes and Prevotella pectinovora increased significantly (P < 0.05). Second, transcriptome sequencing results showed that a total of 434 differentially expressed mRNAs were identified, including 196 up-regulated and 238 down-regulated genes. These differentially expressed genes related to inflammation and immunity, such as GAMA, FABP1, F2RL1 and RSAD2, may play an important role in the process of host resistance to coccidia infection. Functional studies showed that the enriched pathways of differentially expressed genes included the TGF-beta signaling pathway and the ErbB signaling pathways. Finally, the integrated analysis of gut microbiome and host transcriptome suggested that Prevotella pectinovora associated with FABP1, Butyricicoccus porcorum and Colidextribacter sp. associated with RSAD2 were involved in the immune response upon E. tenella infection.

Conclusion

In conclusion, this study provides valuable information on the microbiota and key immune genes after chicken E. tenella infection, with the aim of providing reference for the impact of coccidia infection on cecal microbiome and host.
Association of GSDMD with microvascular-ischemia reperfusion injury after ST-elevation myocardial infarction
Authors: Sun Wenjing , Wang Chunqiu , Cui Shihua , Wang Yan , Zhao Shenghui , Lu Min , Yang Fan , Dong Shujuan , Chu Yingjie
Published: June 2023

Objectives

Little is known about the clinical prognosis of gasdermin D (GSDMD) in patients with ST-elevation myocardial infarction (STEMI). The purpose of this study was to investigate the association of GSDMD with microvascular injury, infarction size (IS), left ventricular ejection fraction (LVEF), and major adverse cardiac events (MACEs), in STEMI patients with primary percutaneous coronary intervention (pPCI).

Methods

We retrospectively analyzed 120 prospectively enrolled STEMI patients (median age 53 years, 80% men) treated with pPCI between 2020 and 2021 who underwent serum GSDMD assessment and cardiac magnetic resonance (CMR) within 48 h post-reperfusion; CMR was also performed at one year follow-up.

Results

Microvascular obstruction was observed in 37 patients (31%). GSDMD concentrations ≧ median (13 ng/L) in patients were associated with a higher risk of microvascular obstruction and IMH (46% vs. 19%, P = 0.003; 31% vs. 13%, P = 0.02, respectively), as well as with a lower LVEF both in the acute phase after infarction (35% vs. 54%, P < 0.001) and in the chronic phase (42% vs. 56%, P < 0.001), larger IS in the acute (32% vs. 15%, P < 0.001) and in the chronic phases (26% vs. 11%, P < 0.001), and larger left ventricular volumes (119 ± 20 vs. 98 ± 14, P = 0.003) by CMR. Univariable and multivariable Cox regression analysis results showed that patients with GSDMD concentrations ≧ median (13 ng/L) had a higher incidence of MACE (P < 0.05).

Conclusions

High GSDMD concentrations in STEMI patients are associated with microvascular injury (including MVO and IMH), which is a powerful MACE predictor. Nevertheless, the therapeutic implications of this relation need further research.
Reassessment of the electrical connection between the pulmonary veins and the left atrium: A study to determine the different contributions of myocardial fibers along the standard ablation circumference
Authors: Campos-García Bieito , Alonso-Martín Concepción , Guerra José M. , Moreno-Weidmann Zoraida , Méndez-Zurita Francisco , Montiel-Quintero Rodolfo , Betancur-Gutiérrez Andrés , Viñolas-Prat Xavier , Rodríguez-Font Enrique
Published: June 2023

Background

Circumferential ablation around the ipsilateral pulmonary veins (PVs) is the standard strategy for atrial fibrillation ablation. The present study seeks to assess which regions of the standard ablation circumference are the main contributors to the venoatrial electrical connection.

Methods

A total of 41 patients were included under a specific atrial fibrillation ablation protocol in which the anterior and posterior segments of the standard circumference, between the equatorial line of the superior and the inferior ipsilateral PVs, were ablated first. If PV isolation was not achieved, ablation was extended superiorly or inferiorly, on the basis of the earliest atrial activation recorded during pacing from inside the PV. Complete PV isolation and the length of the areas not requiring ablation (ANRA) at the time of electrical isolation were evaluated.

Results

Ablation of the anterior and posterior segments of the standard circumference led to the isolation of 77% left-PV pairs and 51% right-PV pairs (p = 0,015). A superior extension was required in 23% left-PV pairs and in 46% right-PV pairs, while an inferior extension was required only in 10% left-PV pairs and in 11% right-PV pairs. PV isolation was achieved before completing the standard ablation circumference in 97% left-PV pairs and in 94% right-PV pairs, with a median ANRA of 36.9 (IQR: 30.9–42.1) mm in the left PVs [16.0 (IQR: 12.0–19.0) mm superior and 18.8 (IQR: 16.1–24.9) mm inferior, p < 0.01] and 36.9 (IQR: 30.2–41.0) mm in the right PVs [15.1 (IQR: 10.7–19.1) mm superior and 20.6 (IQR: 16.9–23.3) mm inferior, p < 0.01].

Conclusions

The myocardial fibers along the anterior and posterior regions of the standard ablation circumference are the main contributors to the electrical connection between the pulmonary veins and the left atrium. Ablation of these regions results in PV isolation in the majority of patients.
Case report: Constrictive pericarditis after coronary artery perforation during percutaneous coronary intervention
Authors: Kim Kyung An , Lee Kwan Yong , Hwang Byung-Hee , Kim Do Yeon , Park Chan Beom
Published: June 2023

A 77-year-old man underwent percutaneous coronary intervention (PCI) at the right coronary artery, which was complicated by coronary artery perforation (CAP). After prolonged balloon tamponade proximal to the CAP there was no more contrast extravasation, and the CAP was thought to have resolved. Computed tomography (CT) and echocardiography the following day did not find evidence of continued bleeding, and the patient was discharged. Echocardiograms and chest CT scans obtained one week and two months after PCI detected no remarkable interval change. The patient complained of progressive dyspnea and abdominal distension seven months after PCI however, and echocardiography found an increased amount of pericardial effusion and constrictive physiology. The patient underwent pericardiectomy due to congestive hepatopathy, and progressive dyspnea. The pericardium was thickened and adhesive, and a dark bloody effusion was found. Pathology was unremarkable except for thick fibrosis. After the operation the patient made full recovery, and is stable three years after surgery.
Characterizing the visualization design space of distant and close reading of poetic rhythm
Authors: Benito-Santos Alejandro , Muñoz Salvador , Therón Sánchez Roberto , García Peñalvo Francisco J.
Published: June 2023

Metrical and rhythmical poetry analysis is founded on the systematic statistical analysis and comparison of sonic devices (e.g., rhythmic patterns) that emerge from a combination of pre-established aesthetic and structural rules and the poet's abilities and creative genius to convey a given message adhering to the said constraints. These rhythmical patterns, which have been traditionally obtained by means of a careful close reading of the poems, in a process known as “scansion,” can now be obtained and made visible by automatic means. However, the visualization literature is still scarce on approaches that allow an insightful close and distant reading of the rhythmical patterns in a poetry corpus. In this work, we report our initial efforts in characterizing of the visualization design space of distant and close reading of poetic rhythm. By employing a digital version of a corpus of 11,268 verses originally written by the Spanish poet and playwright Federico García-Lorca (1898–1936), we could craft several prototypical visualizations representative of the inherent complexity of the problem which we expect to employ in future user studies and that we share here with the rest of the community to foster further discussion around this interesting topic.
Programmed frozen embryo transfer cycles are associated with a higher risk of abnormal placental development: a retrospective cohort study of singleton live births
Authors: Wang Fuxin , Wang Qi , Song Ye , Ding Jie , Li Hong , Meng Qingxia
Published: June 2023

Introduction

Abnormal placental development can lead to adverse outcomes for both mother and fetus. The effect of different types of endometrium preparation regimens of frozen-thawed cycles on the placental development features associated with the perinatal outcomes remains unclear. Hence, we conducted a retrospective cohort study to assess the impact of specific endometrial preparation regimens on placenta-mediated pregnancy complications in singleton live births.

Methods

A retrospective cohort study was conducted evaluating data of all singleton live births both conceived naturally or by in vitro fertilization (IVF) therapy from 2018 to 2020 at our hospital. Two exposed groups of frozen-thawed embryo transfer (FET) were created by the endometrium preparation regimen as the modified natural cycles (mNC) and the programmed cycles. The nonexposed group was the singleton pregnancies conceived naturally. The obstetrical and perinatal outcomes were compared among the three groups using multivariate analysis to adjust the results for determinants potentially associated with the abnormal placental development.

Results

A total of 2186 pregnant women with singleton live births were included in our final analysis and were divided into three groups as naturally conceived group (n=1334), mNC-FETs group (n=217) and programmed-FETs group(n=635). After adjusting for maternal age and parity, no significant difference was observed on the risk of placental disorders between mNC-FET cycles and natural conceived pregnancies (aOR 1.16; 95%CI 1.31-7.01), while programmed-FET cycles were associated with a higher occurrence of placental disorders (aOR 5.36; 95%CI 3.63-8.05). Using the mNC-FET group as a reference and adjusting for confounders such as maternal age, parity, endometrial thickness, and number of embryos transferred, we found that the main manifestation of abnormal placentation in programmed FET cycles was abnormal placental attachment, including placental adhesion and placenta increta (aOR 2.50, 95%CI 1.36-4.90). The dysfunction of placentation in programmed-FET cycles was independently associated with the type of infertility, the total dose of Femostone and thinner endometrium. Additionally, placental disorders in the programmed-FET group were associated with higher rate of preeclampsia, postpartum hemorrhage and Cesarean section.

Conclusion

Our retrospective study revealed that the programmed-FET has a substantial impact on placental development, resulting in a higher incidence of preeclampsia, postpartum hemorrhage and Cesarean section. These findings have significant implications on clinical decision-making.
Risk evaluation and management mechanism of green electricity market based on multi-dimensional cloud model
Authors: Pan Weiwei , Qiao Songbo , Ruan Tingting , Luo Renjie , Li Zhiyi
Published: June 2023

In the process of continuous promotion in the green electricity market, various risks have emerged due to the inherent uncertainty and complex trading environment of the market. Evaluating and controlling the risks through a multi-dimensional evaluation is crucial for the smooth and healthy development of the green electricity market. This work proposes a risk assessment method for the green electricity market based on the multi-dimensional cloud model. First, the comprehensive indicator evaluation system is established for both pre-transaction and post-transaction dimensions by analyzing the operating mechanism of the green electricity market. Second, each dimensional cloud is formed by the evaluation clouds of all indicator levels. The comprehensive risk level cloud is generated based on the indicator values and the evaluation clouds of each indicator. Finally, the sources of market risk are identified, and the corresponding risk management methods are proposed on the basis of the risk evaluation level and membership degree of each indicator. The case study results demonstrate that the proposed method can accurately evaluate and reduce the potential risks existing in the green electricity market through the two-stage detection mechanism.
Decoupling of voltage magnitude and phase in genetic algorithms for smart transmission network state estimation
Authors: Tran Thanh-Son , Kieu Thi-Thanh-Hoa , Le Duc-Tung
Published: June 2023

A large amount of renewable energy resources are integrated into electricity transmission networks. For efficient operation, transmission networks should be smart, in which it is necessary to have the function of estimating the state of the power system. This function determines bus voltage magnitude and phase, which are used for monitoring, operating, and controlling transmission networks. Transmission network state estimation is developed to estimate bus voltage magnitude and phase by many methods, which include genetic algorithms. Published articles have not mentioned in detail how to apply genetic algorithms to estimate the state of large transmission networks, and the application is limited to small networks. Therefore, this work seeks to estimate the state of large transmission networks by genetic algorithms. Strong decoupling was observed between active power and the voltage phase, as well as between reactive power and voltage magnitude, while a weak coupling, between active power and voltage magnitude, as well as between reactive power and voltage phase; voltage magnitude has a value of around 1.0 p.u, and the voltage phase has a value of around 0.0. This paper proposes a novel approach to genetic algorithms for estimating the state of smart transmission networks. In this novel approach, voltage magnitude and phase are separately estimated at each iteration of genetic algorithms. The approach is validated on IEEE 14-, 30-, and 118-bus networks. Results show that our approach can use phasor measurement unit data to estimate the state of large smart transmission networks by genetic algorithms.
Examining green purchasing. The role of environmental concerns, perceptions on climate change, preferences for EU integration, and media exposure
Authors: Hlaváček Martin , Čábelková Inna , Brož David , Smutka Luboš , Prochazka Petr
Published: June 2023

Factors impacting green consumption studied in the literature include 1) economic incentives and possibilities, 2) socio-dem1ographic segmentation, 3) values, emotions and personal responsibilities, 4) information including education and mass media, 5) factors related to the locality of the respondents and the lifestyles. While the effects of environmental concerns and perceptions of climate change or green purchasing are well established, the impacts of preferences for EU integration and media exposure are less clear. The article examines the effects of environmental concerns, perceptions of climate change, trust in EU policies, and media exposition on green purchasing employing a representative sample of 904 respondents (aged 15–95 years, M ± SD: 47.74 ± 17.66; 51.40% women, 19.40% with higher education) in the Czech Republic. Methodologically we rely on principal component analysis, correlations, and a set of ordinal regression analyses. The results suggest that 1) the public perceives the agendas of environment protection and climate change as two different agendas. 2) environment protection attitudes and climate concerns, the acceptance of EU integration positively predict green consumption. 3) the impact of the media exposition proved controversial: printed media and online discussion forums and blogs positively predicted green purchasing, while exposition to online social networks negatively impacted purchasing of organic food; 4) the frequency of watching TV negatively predicted purchasing of environmentally friendly products. We suggest that the advertisements emphasizing low prices may reduce willingness to pay a price premium for green products. It implies that more efforts need to be made on TV and social networks to increase public awareness of green consumption.
First report of Melampsora epitea causing stem cankers on Salix pentandra in Alberta, Canada
Authors: Ramsfield Tod D. , Feau Nicolas , Tanguay Philippe , Hamelin Richard C. , Herath Padmini , Bozic Toso
Published: June 2023

In June, 2021, laurel willow (Salix pentandra) near Slave Lake, Alberta, was found to be infected by a Melampsora sp. that produced bright yellow urediniospores in uredia that were present on catkins, leaves, and stems. All Melampsora species previously reported in Canada are recorded as infecting leaves; therefore, further investigation was undertaken to ascertain the identity of this pathogen. To assess the relationship between this specimen and other Melampsora spp. previously collected from Canada, samples of willow leaves infected by Melampsora spp. were sourced from mycological herbariums located at the Laurentian Forestry Centre (QFB) and the Northern Forestry Centre (CFB, WINF(M)). DNA sequence data from the internal transcribed spacer (ITS) ribosomal RNA region of the fresh specimen, herbarium specimens, and DNA sequence data deposited within GenBank, were used to conduct a phylogenetic analysis. Sequencing and BLAST analysis of the material from the sample resulted in a 99.3% sequence identity match to Melampsora epitea “Mel J” collected from Larix laricina in New York State. The ITS sequence from the herbarium sample WINF(M)7356 (described as M. abieti-capraearum from Manitoba) had 100.0% identity with the Alberta sample. Additionally, specimens WINF(M)11892 (Melampsora sp. from Manitoba) and CFB8931 (Melampsora sp. from the Yukon) had 99.0% sequence identity with the Alberta sample. From these results we applied the identity of M. epitea to the rust discovered in Slave Lake, AB. With the current emphasis on willows for bioenergy production in Canada, growers must remain vigilant for this pathogen and the damages it could cause to willow plantations.
High-throughput sgRNA testing reveals rules for Cas9 specificity and DNA repair in tomato cells
Authors: Slaman Ellen , Lammers Michiel , Angenent Gerco C. , de Maagd Ruud A.
Published: June 2023

CRISPR/Cas9 technology has the potential to significantly enhance plant breeding. To determine the specificity and the mutagenic spectrum of SpCas9 in tomato, we designed 89 g(uide) RNAs targeting genes of the tomato MYB transcription factor family with varying predicted specificities. Plasmids encoding sgRNAs and Cas9 were introduced into tomato protoplasts, and target sites as well as 224 predicted off-target sites were screened for the occurrence of mutations using amplicon sequencing. Algorithms for the prediction of efficacy of the sgRNAs had little predictive power in this system. The analysis of mutations suggested predictable identity of single base insertions. Off-target mutations were found for 13 out of 89 sgRNAs and only occurred at positions with one or two mismatches (at 14 and 3 sites, respectively). We found that PAM-proximal mismatches do not preclude low frequency off-target mutations. Off-target mutations were not found at all 138 positions that had three or four mismatches. We compared off-target mutation frequencies obtained with plasmid encoding sgRNAs and Cas9 with those induced by ribonucleoprotein (RNP) transfections. The use of RNPs led to a significant decrease in relative off-target frequencies at 6 out of 17, no significant difference at 9, and an increase at 2 sites. Additionally, we show that off-target sequences with insertions or deletions relative to the sgRNA may be mutated, and should be considered during sgRNA design. Altogether, our data help sgRNA design by providing insight into the Cas9-induced double-strand break repair outcomes and the occurrence of off-target mutations.
The clinical phenotype with gastrostomy and abdominal wall infection in a pediatric patient with Takenouchi-Kosaki syndrome due to a heterozygous c.191A > G (p.Tyr64Cys) variant in CDC42: a case report
Authors: Szczawińska-Popłonyk Aleksandra , Popłonyk Natalia , Badura-Stronka Magdalena , Juengling Jerome , Huhn Kerstin , Biskup Saskia , Bancerz Bartłomiej , Walkowiak Jarosław
Published: June 2023

The CDC42 (cell division cycle homolog 42) gene product, Cdc42 belongs to the Rho GTPase family which plays a pivotal role in the regulation of multiple cellular functions, including cell cycle progression, motility, migration, proliferation, transcription activation, and reactive oxygen species production. The Cdc42 molecule controls various tissue-specific functional pathways underpinning organogenesis as well as developmental integration of the hematopoietic and immune systems. Heterozygous c.191A>G (p.Tyr64Cys) pathogenic variants in CDC42 cause Takenouchi-Kosaki syndrome characterized by a spectrum of phenotypic features comprising psychomotor developmental delay, sensorineural hearing loss, growth retardation, facial dysmorphism, cardiovascular and urinary tract malformations, camptodactyly, accompanied by thrombocytopenia and immunodeficiency of variable degree. Herein, we report a pediatric patient with the Takenouchi-Kosaki syndrome due to a heterozygous p.Tyr64Cys variant in CDC42 manifesting as a congenital malformation complex accompanied by macrothrombocytopenia, poor specific antibody response, B and T cell immunodeficiency, and low serum immunoglobulin A level. We also suggst that feeding disorders, malnutrition, and a gastrointestinal infection could be a part of the phenotypic characteristics of Takenouchi-Kosaki syndrome supporting the hypothesis of immune dysregulation and systemic inflammation occurring in the p.Tyr64Cys variant in CDC42.
Case report: The spectrum of SMPD1 pathogenic variants in Hungary
Authors: Molnar Maria Judit , Szlepak Tamas , Csürke Ildikó , Loth Szendile , Káposzta Rita , Erdős Melinda , Dezsőfi Antal
Published: June 2023

Acid sphingomyelinase deficiency (ASMD) is an autosomal recessive disease caused by biallelic pathogenic variants in the sphingomyelin phosphodiesterase-1 (SMPD1) gene. Acid sphingomyelinase deficiency is characterized by a spectrum of disease and is broadly divided into three types (ASMD type A, ASMD type A/B, and ASMD type B). More than 220 disease-associated SMPD1 variants have been reported, and genotype/phenotype correlations are limited. Here we report the first description of all six diagnosed acid sphingomyelinase deficiency cases in Hungary. Nine SMPD1 variants are present in this cohort, including 3 SMPD1 variants (G247D, M384R, and F572L), which have only been described in Hungarian patients. All described variants are deemed to be pathogenic. Eight of the variants are missense, and one is a frameshift variant. The treatment of an ASMD type A/B patient in this cohort using hematopoietic stem cell transplantation is also detailed. This study may help to support diagnosis, patient genetic counseling, and management of acid sphingomyelinase deficiency.
CD24-Fc suppression of immune related adverse events in a therapeutic cancer vaccine model of murine neuroblastoma
Authors: Wu Xiaofang , Srinivasan Priya , Basu Mousumi , Zimmerman Talia , Li Samuel , Wang Yin , Zheng Pan , Liu Yang , Sandler Anthony David
Published: June 2023

Introduction

The combination of Myc-suppressed whole tumor cells with checkpoint inhibitors targeting CTLA-4 and PD-L1 generates a potent therapeutic cancer vaccine in a mouse neuroblastoma model. As immunotherapies translate from pre-clinical to clinical trials, the potential immune-related adverse events (irAEs) associated with induction of potent immunity must be addressed. The CD24-Siglec 10/G interaction is an innate checkpoint that abrogates inflammatory responses to molecules released by damaged cells, but its role in cancer immunology is not well defined. We investigate irAEs of an effective whole cell neuroblastoma vaccine and subsequently the effect of CD24-Fc, a CD24 and Fc fusion protein, on both the vaccine efficacy and induced irAEs in a mouse neuroblastoma model.

Methods

To test whether the whole tumor cell vaccination leads to autoimmune responses in other organ systems we harvested lung, heart, kidney and colon from naïve mice (n=3), unvaccinated tumor only mice (n=3), and vaccinated mice with CD24 Fc (n=12) or human IgG-Fc control (n=12) after tumor inoculation and vaccination therapy at day 30. The Immune cell infiltrates and immunogenic pathway signatures in different organ systems were investigated using NanoString Autoimmune Profiling arrays. Nanostring RNA transcript results were validated with immunohistochemistry staining.

Results

The whole tumor cell vaccine combined with immune checkpoint therapy triggers occult organ specific immune cell infiltrates, primarily in cardiac tissue and to a lesser extent in the renal and lung tissue, but not in the colon. CD24-Fc administration with vaccination partially impedes anti-tumor immunity but delaying CD24-Fc administration after initial vaccination reverses this effect. CD24-Fc treatment also ameliorates the autoimmune response induced by effective tumor vaccination in the heart.

Discussion

This study illustrates that the combination of Myc suppressed whole tumor cell vaccination with checkpoint inhibitors is an effective therapy, but occult immune infiltrates are induced in several organ systems in a mouse neuroblastoma model. The systemic administration of CD24-Fc suppresses autoimmune tissue responses, but appropriate timing of administration is critical for maintaining efficacy of the therapeutic vaccine.