bluuebunny/crossref_metadata_2025 huggingface dataset:
LO061: Variation in emergency department use of computed tomography for investigation of acute aortic dissection
Authors: Ohle R. , Luo L. , Anjum O. , Bleeker H. , Perry J.J.
Published: June 2016

Introduction: Acute aortic dissection (AAD) is a life threatening condition making early diagnosis critical. Although 90% present with acute pain, the myriad of associated symptoms can make investigation and diagnosis a challenge. Our objectives were to assess emergency physician use of CT, yield of CT and ordering variation among physicians in patients presenting with pain for diagnosis of AAD. Methods: This historical cohort study of consecutive adult patients presenting to two tertiary academic care EDs over one calendar year included patients with a primary complaint of non-traumatic chest, back, abdominal or flank pain. Patients were excluded if clear diagnosis was made by basic investigations or exam. Primary outcome was rate of CT Thorax or Thorax/Abdomen ordered to rule out AAD as per clinical indication on diagnostic requisition. Secondary outcome was variation in CT ordering. Variation was measured with; Cochrane q test for homogeneity, proportion of positive CT’s (z-test) and mean CT’s (t test) ordered between high (>5CT/yr) and low (<5CT/yr) test users. Sample size of 6 per group was calculated based on an expected delta in mean CT ordered of 5 and a within group SD of 3. Results: 31,201 patients presented with chest, abdominal, back, flank pain during the study period. 8,472 were excluded based on a diagnosis made by clinical exam or basic investigations. 22,776 were included (Mean 47years SD 18.5yrs 56.2% Female). Most common diagnoses; Chest pain NYD(23.3%), Abdominal pain NYD(20.8%), Lower back pain NYD(10.5%), Renal Colic (5.3%), ACS (2.9%). CT was ordered to rule out AAD in 175 (0.7%) (Mean 62 years SD 16.5, 50.6% Female). Only 4(2.3%) were found to have an AAD. There was significant variation (range 0.6-12% Q test P<0.027) between proportion of CT's ordered by physicians. Between high (Mean 7.9 n=10 AAD=2) and low test users (Mean 2.3 n=41 AAD=2), there was significant difference in mean number of CT’s ordered (p<0.001) but no difference in number of AAD found (p<0.2). No AAD were missed. Conclusion: Current rate of imaging for aortic dissection is appropriately low but inefficient, with 98% of advanced imaging negative. There is significant variation in physician CT ordering (almost 20-fold) without an increase in diagnosis. These findings suggest great potential for more standardized and efficient use of CT for the diagnosis of AAD.
Effects of different sources of forage in high-concentrate diets on fermentation parameters, ruminal biohydrogenation and microbiota in Nellore feedlot steers
Authors: MESSANA J. D. , CARVALHO A. L. E. G. F. , RIBEIRO A. F. , FIORENTINI G. , CASTAGNINO P. S. , GRANJA-SALCEDO Y. T. , PIRES A. V. , BERCHIELLI T. T.
Published: June 2016

Modifying the extent of fatty acid (FA) biohydrogenation (BH) in the rumen through diet formulation is an effective strategy for changing the content of unsaturated FAs (USFAs) in meat. The present study investigated the effects of different sources of forage in high-concentrate diets on intake, digestibility, rumen fermentation, ruminal BH, duodenal flow of FAs and rumen microbiota in Nellore steers. Intake of neutral detergent fibre (NDF) was higher in animals fed with maize silage (MS) than in those fed with sugar cane (SC) and sugar cane bagasse (SB). Higher digestibility of dry matter and NDF was found in animals fed with MS than in those fed with the other diets. In addition, higher crude protein digestibility was observed in animals fed with sugar cane bagasse than in those fed with SC. Non-fibre carbohydrate (NFC) digestibility was higher in animals fed with sugar cane than in those fed with the other diets. Intake of total and individual FAs such as C18 : 1cis-9, C18 : 2, and C18 : 3 was similar between animals fed with MS and SB, but decreased in animals fed with SC. Diets containing MS and SB showed higher total digestibility of saturated FAs (SFAs) and USFAs, respectively and total FAs and ruminal BH of C18 : 1 and USFA. Intestinal digestibility of overall FAs did not differ among treatments, except for C18 : 3, which increased in animals fed with SC and SB. The profile of FAs in duodenal digesta and faecal outputs did not differ among treatments. However, the flow of NDF was higher in animals fed with SC than in those fed with MS and SB. Animals fed with SB showed higher values of pH than those fed with MS and SC. Animals fed with SC showed lower values of ammonia-nitrogen. Protozoan counts were only influenced by diet for species that belonged to the generaDasytrichaandIsotricha. Populations of fibrolytic bacteria (Ruminococus flavefaciens, Ruminococus albusandFibrobacter succinogenes) were similar among diets. Populations ofSelenomonas ruminantiumincreased 2·5 and 5 times in animals fed with MS when compared with those fed with SC and SB, respectively. The use of MS increased intake and digestibility of NDF, and the use of SC decreased ruminal BH of total USFA without changing the flow of FAs to the duodenum. Thus, different sources of forage in high-concentrate diets do not modify the duodenal flow of USFA or fibrolytic bacteria. This must be taken into account when formulating diets to modulate ruminal upsets without altering intake.
Knowledge and Security
Authors: Le Morvan Pierre
Published: June 2016

The central aim of this paper is to revive and refine an idea inspired by Plato, and to show how it can be developed into a plausible contemporary theory on which factive knowledge is secure true belief. In so doing, I disentangle two Platonic (or at least inspired by Plato) ideas: that knowledge is secure true belief, and that knowledge is true belief secured by a logos. I defend the former but not the latter. My defence involves distinguishing between alethic and doxastic security, and arguing for understanding factive knowledge in terms of both.
Impacts of land use and infrastructural changes on threatened Little Bustard Tetrax tetrax breeding populations: quantitative assessments using a recently developed spatially explicit dynamic modelling framework
Authors: SANTOS MÁRIO , BESSA REGINA , CABRAL JOÃO ALEXANDRE , PACHECO FERNANDO ANTÓNIO LEAL , LEITÃO DOMINGOS , MOREIRA FRANCISCO , PINTO MÁRCIA , LECOQ MIGUEL , SILVA JOÃO PAULO
Published: June 2016

With the combination of worldwide landscape changes and the uncertainty about the impact on species abundance and distribution, the value of spatio-temporal modelling tools is increasingly obvious. The Little Bustard Tetrax tetrax breeds on low-intensity arable cultivation and pastoral land and is currently threatened by diverse landscape modifications. The aim of this research was to predict Little Bustard population trends in the face of realistic scenarios of land use and infrastructure changes, applying a recently developed spatially explicit framework, based on the stochastic dynamic methodology (StDM). The application of this approach provided some basis to analyse the responses of breeding populations’ spatial distribution and abundance to the scenarios implemented. Since some of these scenarios represent local and/or regional risks to the viability of Little Bustard breeding populations, the results obtained demonstrate the potential of the proposed framework for landscape planning in the scope of the conservation of this threatened species. This approach also provides a promising baseline to support ecological risk assessments for other species, derived from ecological models with increased predictive power and intuitiveness to decision makers and environmental managers.
Pharaoh's Iron Dagger Made from a Meteorite, Study Confirms
Authors: Deatrick Elizabeth
Published: June 2016

After examining the metal under bombardment by X-rays, scientists find the composition of King Tutankhamun's knife blade matches "iron of the sky."
Guttiferone K impedes cell cycle re-entry of quiescent prostate cancer cells via stabilization of FBXW7 and subsequent c-MYC degradation
Authors: Xi Z , Yao M , Li Y , Xie C , Holst J , Liu T , Cai S , Lao Y , Tan H , Xu H-X , Dong Q
Published: June 2016

Cell cycle re-entry by quiescent cancer cells is an important mechanism for cancer progression. While high levels of c-MYC expression are sufficient for cell cycle re-entry, the modality to block c-MYC expression, and subsequent cell cycle re-entry, is limited. Using reversible quiescence rendered by serum withdrawal or contact inhibition in PTENnull/p53WT (LNCaP) or PTENnull/p53mut (PC-3) prostate cancer cells, we have identified a compound that is able to impede cell cycle re-entry through c-MYC. Guttiferone K (GUTK) blocked resumption of DNA synthesis and preserved the cell cycle phase characteristics of quiescent cells after release from the quiescence. In vehicle-treated cells, there was a rapid increase in c-MYC protein levels upon release from the quiescence. However, this increase was inhibited in the presence of GUTK with an associated acceleration in c-MYC protein degradation. The inhibitory effect of GUTK on cell cycle re-entry was significantly reduced in cells overexpressing c-MYC. The protein level of FBXW7, a subunit of E3 ubiquitin ligase responsible for degradation of c-MYC, was reduced upon the release from the quiescence. In contrast, GUTK stabilized FBXW7 protein levels during release from the quiescence. The critical role of FBXW7 was confirmed using siRNA knockdown, which impaired the inhibitory effect of GUTK on c-MYC protein levels and cell cycle re-entry. Administration of GUTK, either in vitro prior to transplantation or in vivo, suppressed the growth of quiescent prostate cancer cell xenografts. Furthermore, elevation of FBXW7 protein levels and reduction of c-MYC protein levels were found in the xenografts of GUTK-treated compared with vehicle-treated mice. Hence, we have identified a compound that is capable of impeding cell cycle re-entry by quiescent PTENnull/p53WT and PTENnull/p53mut prostate cancer cells likely by promoting c-MYC protein degradation through stabilization of FBXW7. Its usage as a clinical modality to prevent prostate cancer progression should be further evaluated.
Role of long non-coding RNA-RNCR3 in atherosclerosis-related vascular dysfunction
Authors: Shan K , Jiang Q , Wang X -Q , Wang Y -N -Z , Yang H , Yao M -D , Liu C , Li X -M , Yao J , Liu B , Zhang Y -Y , J Yong , Yan B
Published: June 2016

Atherosclerosis is one of the most common vascular disorders. Endothelial cell (EC) dysfunction and vascular smooth muscle cell (VSMC) proliferation contributes to the development of atherosclerosis. Long non-coding RNAs (lncRNAs) have been implicated in several biological processes and human diseases. Here we show that lncRNA-RNCR3 is expressed in ECs and VSMCs. RNCR3 expression is significantly upregulated in mouse and human aortic atherosclerotic lesions, and cultured ECs and VSMCs upon ox-LDL treatment in vitro. RNCR3 knockdown accelerates the development of atherosclerosis, aggravates hypercholesterolemia and inflammatory factor releases, and decreases EC and VSMC proliferation in vivo. RNCR3 knockdown also reduces the proliferation and migration, and accelerates apoptosis development of EC and VSMC in vitro. RNCR3 acts as a ceRNA, and forms a feedback loop with Kruppel-like factor 2 and miR-185-5p to regulate cell function. This study reveals that RNCR3 has an atheroprotective role in atherosclerosis, and its intervention is a promising strategy for treating atherosclerosis-related vascular dysfunction.
Proteolytic degradation and potential role of onconeural protein cdr2 in neurodegeneration
Authors: Hwang J-Y , Lee J , Oh C-K , Kang H W , Hwang I-Y , Um J W , Park H C , Kim S , Shin J-H , Park W-Y , Darnell R B , Um H-D , Chung K C , Kim K , Oh Y J
Published: June 2016

Cerebellar degeneration-related protein 2 (cdr2) is expressed in the central nervous system, and its ectopic expression in tumor cells of patients with gynecological malignancies elicits immune responses by cdr2-specific autoantibodies and T lymphocytes, leading to neurological symptoms. However, little is known about the regulation and function of cdr2 in neurodegenerative diseases. Because we found that cdr2 is highly expressed in the midbrain, we investigated the role of cdr2 in experimental models of Parkinson’s disease (PD). We found that cdr2 levels were significantly reduced after stereotaxic injection of 1-methyl-4-phenylpyridinium (MPP+) into the striatum. cdr2 levels were also decreased in the brains of post-mortem PD patients. Using primary cultures of mesencephalic neurons and MN9D cells, we confirmed that MPP+ reduces cdr2 in tyrosine hydroxylase-positive dopaminergic neuronal cells. The MPP+-induced decrease of cdr2 was primarily caused by calpain- and ubiquitin proteasome system-mediated degradation, and cotreatment with pharmacological inhibitors of these enzymes or overexpression of calcium-binding protein rendered cells less vulnerable to MPP+-mediated cytotoxicity. Consequently, overexpression of cdr2 rescued cells from MPP+-induced cytotoxicity, whereas knockdown of cdr2 accelerated toxicity. Collectively, our findings provide insights into the novel regulatory mechanism and potentially protective role of onconeural protein during dopaminergic neurodegeneration.
Endoplasmic reticulum stress eIF2α–ATF4 pathway-mediated cyclooxygenase-2 induction regulates cadmium-induced autophagy in kidney
Authors: Luo B , Lin Y , Jiang S , Huang L , Yao H , Zhuang Q , Zhao R , Liu H , He C , Lin Z
Published: June 2016

The heavy metal cadmium (Cd) is nephrotoxic. Recent studies show that autophagy plays an essential role in Cd-induced kidney injury. However, the mechanisms of Cd-induced kidney injury accompanied by autophagy are still obscure. In the present study, we first confirmed that Cd induced kidney damage and dysfunction, along with autophagy, both in vivo and in vitro. Then, we observed that cyclooxygenase-2 (COX-2) and the eIF2α–ATF4 pathway of endoplasmic reticulum (ER) stress were induced by Cd in both kidney tissues and cultured cells. Further studies showed that inhibition of COX-2 with celecoxib or RNA interference (RNAi) inhibited the Cd-induced autophagy in kidney cells. In addition, blocking ER stress with 4-phenylbutyrate or RNAi partially counteracted COX-2 overexpression and autophagy induced by Cd, which suggested that ER stress was required for Cd-induced kidney autophagy. Significantly, our results showed that Cd activated ATF4 and induced its translocation to the nucleus. Knockdown of ATF4 inhibited Cd-induced COX-2 overexpression. While COX-2 overexpression is involved in renal dysfunction, there is no prior report on the role of COX-2 in autophagy regulation. The results of the current study suggest a novel molecular mechanism that the ER stress eIF2α–ATF4 pathway-mediated COX-2 overexpression contributes to Cd-induced kidney autophagy and injury. The present study implies that COX-2 may be a potential target for therapy against Cd-induced nephrotoxicity.
Fast, greener and scalable direct coupling of organolithium compounds with no additional solvents
Authors: Pinxterhuis Erik B. , Giannerini Massimo , Hornillos Valentín , Feringa Ben L.
Published: June 2016

Although the use of catalytic rather than stoichiometric amounts of metal mediator in cross-coupling reactions between organic halides and organometallic counterparts improves significantly the atom economy and waste production, the use of solvents and stoichiometric generation of main-group byproducts (B, Sn and Zn) hamper the ‘greenness’ and industrial efficiency of these processes. Here we present a highly selective and green Pd-catalysed cross-coupling between organic halides and organolithium reagents proceeding without additional solvents and with short reaction times (10 min). This method bypasses a number of challenges previously encountered in Pd-catalysed cross-coupling with organolithium compounds such as strict exclusion of moisture, dilution and slow addition. Operational ease of this protocol combines the use of industrially viable catalysts loadings (down to 0.1 mol%), scalability of the process (tested up to 120 mmol) and exceptionally favourable environmental impact (E factors in several cases as low as 1).
Shifting El Niño inhibits summer Arctic warming and Arctic sea-ice melting over the Canada Basin
Authors: Hu Chundi , Yang Song , Wu Qigang , Li Zhenning , Chen Junwen , Deng Kaiqiang , Zhang Tuantuan , Zhang Chengyang
Published: June 2016

Arctic climate changes include not only changes in trends and mean states but also strong interannual variations in various fields. Although it is known that tropical-extratropical teleconnection is sensitive to changes in flavours of El Niño, whether Arctic climate variability is linked to El Niño, in particular on interannual timescale, remains unclear. Here we demonstrate for the first time a long-range linkage between central Pacific (CP) El Niño and summer Arctic climate. Observations show that the CP warming related to CP El Niño events deepens the tropospheric Arctic polar vortex and strengthens the circumpolar westerly wind, thereby contributing to inhibiting summer Arctic warming and sea-ice melting. Atmospheric model experiments can generally capture the observed responses of Arctic circulation and robust surface cooling to CP El Niño forcing. We suggest that identification of the equator-Arctic teleconnection, via the ‘atmospheric bridge’, can potentially contribute to improving the skill of predicting Arctic climate.
ZBTB7A mutations in acute myeloid leukaemia with t(8;21) translocation
Authors: Hartmann Luise , Dutta Sayantanee , Opatz Sabrina , Vosberg Sebastian , Reiter Katrin , Leubolt Georg , Metzeler Klaus H. , Herold Tobias , Bamopoulos Stefanos A. , Bräundl Kathrin , Zellmeier Evelyn , Ksienzyk Bianka , Konstandin Nikola P. , Schneider Stephanie , Hopfner Karl-Peter , Graf Alexander , Krebs Stefan , Blum Helmut , Middeke Jan Moritz , Stölzel Friedrich , Thiede Christian , Wolf Stephan , Bohlander Stefan K. , Preiss Caroline , Chen-Wichmann Linping , Wichmann Christian , Sauerland Maria Cristina , Büchner Thomas , Berdel Wolfgang E. , Wörmann Bernhard J. , Braess Jan , Hiddemann Wolfgang , Spiekermann Karsten , Greif Philipp A.
Published: June 2016

The t(8;21) translocation is one of the most frequent cytogenetic abnormalities in acute myeloid leukaemia (AML) and results in the RUNX1/RUNX1T1 rearrangement. Despite the causative role of the RUNX1/RUNX1T1 fusion gene in leukaemia initiation, additional genetic lesions are required for disease development. Here we identify recurring ZBTB7A mutations in 23% (13/56) of AML t(8;21) patients, including missense and truncating mutations resulting in alteration or loss of the C-terminal zinc-finger domain of ZBTB7A. The transcription factor ZBTB7A is important for haematopoietic lineage fate decisions and for regulation of glycolysis. On a functional level, we show that ZBTB7A mutations disrupt the transcriptional repressor potential and the anti-proliferative effect of ZBTB7A. The specific association of ZBTB7A mutations with t(8;21) rearranged AML points towards leukaemogenic cooperativity between mutant ZBTB7A and the RUNX1/RUNX1T1 fusion.
The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts
Authors: Liu Zhanjiang , Liu Shikai , Yao Jun , Bao Lisui , Zhang Jiaren , Li Yun , Jiang Chen , Sun Luyang , Wang Ruijia , Zhang Yu , Zhou Tao , Zeng Qifan , Fu Qiang , Gao Sen , Li Ning , Koren Sergey , Jiang Yanliang , Zimin Aleksey , Xu Peng , Phillippy Adam M. , Geng Xin , Song Lin , Sun Fanyue , Li Chao , Wang Xiaozhu , Chen Ailu , Jin Yulin , Yuan Zihao , Yang Yujia , Tan Suxu , Peatman Eric , Lu Jianguo , Qin Zhenkui , Dunham Rex , Li Zhaoxia , Sonstegard Tad , Feng Jianbin , Danzmann Roy G. , Schroeder Steven , Scheffler Brian , Duke Mary V. , Ballard Linda , Kucuktas Huseyin , Kaltenboeck Ludmilla , Liu Haixia , Armbruster Jonathan , Xie Yangjie , Kirby Mona L. , Tian Yi , Flanagan Mary Elizabeth , Mu Weijie , Waldbieser Geoffrey C.
Published: June 2016

Catfish represent 12% of teleost or 6.3% of all vertebrate species, and are of enormous economic value. Here we report a high-quality reference genome sequence of channel catfish (Ictalurus punctatus), the major aquaculture species in the US. The reference genome sequence was validated by genetic mapping of 54,000 SNPs, and annotated with 26,661 predicted protein-coding genes. Through comparative analysis of genomes and transcriptomes of scaled and scaleless fish and scale regeneration experiments, we address the genomic basis for the most striking physical characteristic of catfish, the evolutionary loss of scales and provide evidence that lack of secretory calcium-binding phosphoproteins accounts for the evolutionary loss of scales in catfish. The channel catfish reference genome sequence, along with two additional genome sequences and transcriptomes of scaled catfishes, provide crucial resources for evolutionary and biological studies. This work also demonstrates the power of comparative subtraction of candidate genes for traits of structural significance.
Giant Seebeck effect in Ge-doped SnSe
Authors: Gharsallah M. , Serrano-Sánchez F. , Nemes N. M. , Mompeán F. J. , Martínez J. L. , Fernández-Díaz M. T. , Elhalouani F. , Alonso J. A.
Published: June 2016

Thermoelectric materials may contribute in the near future as new alternative sources of sustainable energy. Unprecedented thermoelectric properties in p-type SnSe single crystals have been recently reported, accompanied by extremely low thermal conductivity in polycrystalline samples. In order to enhance thermoelectric efficiency through proper tuning of this material we report a full structural characterization and evaluation of the thermoelectric properties of novel Ge-doped SnSe prepared by a straightforward arc-melting method, which yields nanostructured polycrystalline samples. Ge does not dope the system in the sense of donating carriers, yet the electrical properties show a semiconductor behavior with resistivity values higher than that of the parent compound, as a consequence of nanostructuration, whereas the Seebeck coefficient is higher and thermal conductivity lower, favorable to a better ZT figure of merit.
Wide angle and narrow-band asymmetric absorption in visible and near-infrared regime through lossy Bragg stacks
Authors: Shu Shiwei , Zhan Yawen , Lee Chris , Lu Jian , Li Yang Yang
Published: June 2016

Absorber is an important component in various optical devices. Here we report a novel type of asymmetric absorber in the visible and near-infrared spectrum which is based on lossy Bragg stacks. The lossy Bragg stacks can achieve near-perfect absorption at one side and high reflection at the other within the narrow bands (several nm) of resonance wavelengths, whereas display almost identical absorption/reflection responses for the rest of the spectrum. Meanwhile, this interesting wavelength-selective asymmetric absorption behavior persists for wide angles, does not depend on polarization and can be ascribed to the lossy characteristics of the Bragg stacks. Moreover, interesting Fano resonance with easily tailorable peak profiles can be realized using the lossy Bragg stacks.
miR-146a is essential for lipopolysaccharide (LPS)-induced cross-tolerance against kidney ischemia/reperfusion injury in mice
Authors: Dai Yan , Jia Ping , Fang Yi , Liu Hong , Jiao Xiaoyan , He John C. , Ding Xiaoqiang
Published: June 2016

MicroRNA-146a is one of most important microRNAs involved in development of endotoxin tolerance via (toll-like receptors) TLRs/ NF-κB pathway. In this study, we sought to identify the mechanistic role of miR-146a in mediating the protective effect of lipopolysaccharide (LPS) pretreatment on kidney ischemia/reperfusion injury. A locked nucleic acid–modified anti-miR-146a given before LPS treatment knocked down miR-146a expression and completely negated LPS-mediated protection against kidney ischemia/reperfusion injury. Knockdown of miR-146a resulted in significantly higher histopathological scores for tubular damage, expression of proinflammatory cytokines and chemokines and neutrophil and macrophage infiltration. Furthermore, knockdown of miR-146a greatly up-regulated the protein levels of IL-1 receptor-associated kinase (IRAK-1) and tumor-necrosis factor (TNF) receptor-associated factor 6 (TRAF6), which are known target genes of miR-146a, leading to activation of NF-κB. Finally, elevation of nuclear translocation of NF-κB p65/p50 and caspase-3 expression, degradation of cytosolic IkBα and BcL-xL and substantially exacerbation of tubular cell apoptosis were inversely correlated with miR-146a expression. Taken together, our results identify that miR146a exerts a kidney protective effect through negative regulation of acute inflammatory response by suppressing NF-κB activation and proinflammatory genes expression.
Proteomic Response and Quality Maintenance in Postharvest Fruit of Strawberry (Fragaria × ananassa) to Exogenous Cytokinin
Authors: Li Li , Li Dongdong , Luo Zisheng , Huang Xinhong , Li Xihong
Published: June 2016

The limitations in current understanding of the molecular mechanisms underlying fruit response to the application of plant growth regulators have increasingly become major challenges in improvement of crop quality. This study aimed to evaluate the response of strawberry to the preharvest application of exogenous cytokinin known as forchlorfenuron (CPPU). Postharvest internal and physiological quality attributes were characterized following storage under different conditions. Hierarchical clustering analysis via a label-free proteomic quantitative approach identified a total of 124 proteins in strawberries across all treatments. The expression profiles of both proteins and genes spanned the ranged role of cytokinin involved in primary and secondary metabolism, stress response and so on. Eighty-eight proteins and fifty-six proteins were significantly regulated immediately at harvest and after storage, respectively. In general, the glycolysis in strawberry was only regulated by CPPU before storage; in addition to the accelerated photosynthesis and acid metabolism, CPPU application maintained higher capacity of resistance in strawberry to stress stimuli after storage, in comparison to control. Nevertheless, the volatile biosynthesis in strawberry has been suppressed by exogenous CPPU. Novel cytokinin response proteins and processes were identified in addition to the main transcriptomic expression to gain insights into the phytohormone control of fruit postharvest quality.
Tunable ultraviolet and blue light generation from Nd:YAB random laser bolstered by second-order nonlinear processes
Authors: Moura André L. , Carreño Sandra J. M. , Pincheira Pablo I. R. , Fabris Zanine V. , Maia Lauro J. Q. , Gomes Anderson S. L. , de Araújo Cid B.
Published: June 2016

Ultraviolet and blue light were obtained by nonlinear frequency conversion in a random laser (RL) based on Nd0.10Y0.90Al3(BO3)4 nanocrystalline powder. RL operation at 1062 nm, due to the 4F3/2 → 4I11/2 transition of neodymium ions (Nd3+), was achieved by exciting the Nd3+ with a tunable beam from 680 to 920 nm covering the ground state absorption transitions to the 4F9/2, (4F7/2,4S3/2), (4F5/2,2H9/2) and 4F3/2 states. Light from 340 to 460 nm was obtained via the second-harmonic generation of the excitation beam while tunable blue light, from 417 to 486 nm, was generated by self-sum-frequency mixing between the excitation beam and the RL emission.
Concurrent enhancement of percolation and synchronization in adaptive networks
Authors: Eom Young-Ho , Boccaletti Stefano , Caldarelli Guido
Published: June 2016

Co-evolutionary adaptive mechanisms are not only ubiquitous in nature, but also beneficial for the functioning of a variety of systems. We here consider an adaptive network of oscillators with a stochastic, fitness-based, rule of connectivity and show that it self-organizes from fragmented and incoherent states to connected and synchronized ones. The synchronization and percolation are associated to abrupt transitions and they are concurrently (and significantly) enhanced as compared to the non-adaptive case. Finally we provide evidence that only partial adaptation is sufficient to determine these enhancements. Our study, therefore, indicates that inclusion of simple adaptive mechanisms can efficiently describe some emergent features of networked systems’ collective behaviors and suggests also self-organized ways to control synchronization and percolation in natural and social systems.
Altered resting-state functional activity in posttraumatic stress disorder: A quantitative meta-analysis
Authors: Wang Ting , Liu Jia , Zhang Junran , Zhan Wang , Li Lei , Wu Min , Huang Hua , Zhu Hongyan , Kemp Graham J. , Gong Qiyong
Published: June 2016

Many functional neuroimaging studies have reported differential patterns of spontaneous brain activity in posttraumatic stress disorder (PTSD), but the findings are inconsistent and have not so far been quantitatively reviewed. The present study set out to determine consistent, specific regional brain activity alterations in PTSD, using the Effect Size Signed Differential Mapping technique to conduct a quantitative meta-analysis of resting-state functional neuroimaging studies of PTSD that used either a non-trauma (NTC) or a trauma-exposed (TEC) comparison control group. Fifteen functional neuroimaging studies were included, comparing 286 PTSDs, 203 TECs and 155 NTCs. Compared with NTC, PTSD patients showed hyperactivity in the right anterior insula and bilateral cerebellum, and hypoactivity in the dorsal medial prefrontal cortex (mPFC); compared with TEC, PTSD showed hyperactivity in the ventral mPFC. The pooled meta-analysis showed hypoactivity in the posterior insula, superior temporal, and Heschl’s gyrus in PTSD. Additionally, subgroup meta-analysis (non-medicated subjects vs. NTC) identified abnormal activation in the prefrontal-limbic system. In meta-regression analyses, mean illness duration was positively associated with activity in the right cerebellum (PTSD vs. NTC), and illness severity was negatively associated with activity in the right lingual gyrus (PTSD vs. TEC).
Energy transfer from an individual silica nanoparticle to graphene quantum dots and resulting enhancement of photodetector responsivity
Authors: Kim Sung , Shin Dong Hee , Kim Jungkil , Jang Chan Wook , Kang Soo Seok , Kim Jong Min , Kim Ju Hwan , Lee Dae Hun , Kim Jung Hyun , Choi Suk-Ho , Hwang Sung Won
Published: June 2016

Förster resonance energy transfer (FRET), referred to as the transfer of the photon energy absorbed in donor to acceptor, has received much attention as an important physical phenomenon for its potential applications in optoelectronic devices as well as for the understanding of some biological systems. If one-atom-thick graphene is used for donor or acceptor, it can minimize the separation between donor and acceptor, thereby maximizing the FRET efficiency (EFRET). Here, we report first fabrication of a FRET system composed of silica nanoparticles (SNPs) and graphene quantum dots (GQDs) as donors and acceptors, respectively. The FRET from SNPs to GQDs with an EFRET of ∼78% is demonstrated from excitation-dependent photoluminescence spectra and decay curves. The photodetector (PD) responsivity (R) of the FRET system at 532 nm is enhanced by 100∼101/102∼103 times under forward/reverse biases, respectively, compared to the PD containing solely GQDs. This remarkable enhancement is understood by network-like current paths formed by the GQDs on the SNPs and easy transfer of the carriers generated from the SNPs into the GQDs due to their close attachment. The R is 2∼3 times further enhanced at 325 nm by the FRET effect.
Thiazolides Elicit Anti-Viral Innate Immunity and Reduce HIV Replication
Authors: Trabattoni Daria , Gnudi Federica , Ibba Salomè V. , Saulle Irma , Agostini Simone , Masetti Michela , Biasin Mara , Rossignol Jean-Francois , Clerici Mario
Published: June 2016

Nitazoxanide (Alinia®, NTZ) and tizoxanide (TIZ), its active circulating metabolite, belong to a class of agents known as thiazolides (TZD) endowed with broad anti-infective activities. TIZ and RM-4848, the active metabolite of RM-5038, were shown to stimulate innate immunity in vitro. Because natural resistance to HIV-1 infection in HIV-exposed seronegative (HESN) individuals is suggested to be associated with strong innate immune responses, we verified whether TIZ and RM-4848 could reduce the in vitro infectiousness of HIV-1. Peripheral blood mononuclear cells (PBMCs) from 20 healthy donors were infected in vitro with HIV-1BaL in the presence/absence of TIZ or RM4848. HIV-1 p24 were measured at different timepoints. The immunomodulatory abilities of TZD were evaluated by the expression of type I IFN pathway genes and the production of cytokines and chemokines. TZD drastically inhibited in vitro HIV-1 replication (>87%). This was associated with the activation of innate immune responses and with the up-regulation of several interferon-stimulated genes (ISGs), including those involved in cholesterol pathway, particularly the cholesterol-25 hydroxylase (CH25H). TZD inhibition of HIV-1 replication in vitro could be due to their ability to stimulate potent and multifaceted antiviral immune responses. These data warrant the exploration of TZD as preventive/therapeutic agent in HIV infection.
Dissecting the role of transforming growth factor-β1 in topmouth culter immunobiological activity: a fundamental functional analysis
Authors: Qi Pengzhi , Xie Congxin , Guo Baoying , Wu Changwen
Published: June 2016

Transforming growth factor-β1 (TGF-β1) has been proven to function primarily in mammalian immunobiological activity, but information regarding the immune role of TGF-β1 in teleosts is limited. In the present study, we describe the cDNA cloning and characterization of the TGF-β1 molecule in the topmouth culter. TGF-β1 is highly expressed in immune-related tissues of the culter, including the thymus, head kidney, and spleen. The recombinant culter TGF-β1 (cTGF-β1) was successfully expressed and purified in vitro, and the effects of cTGF-β1 on the mRNA expression of pro-inflammatory cytokines, such as TNF-α and IL-1β, in the absence or presence of LPS was determined in culter peripheral blood leukocytes. cTGF-β1 was found to have bipolar properties in inflammatory reactions. Additionally, to assess the immune role of teleost TGF-β1 in vivo, the expression of TGF-β1 in the culter thymus and spleen tissues induced by poly I:C were also examined. The expression of TGF-β1 was obviously up-regulated, as shown in the cell lines. However, the peak time of cTGF-β1 expression in the cell lines occurred significantly earlier than in the organic tissues under the same inducer, suggesting that the response of the teleost TGF-β1 molecule to exogenous infection depends on a more complicated signalling pathway in vivo than in vitro.
Direct observation of polar tweed in LaAlO3
Authors: Salje Ekhard K. H. , Alexe Marin , Kustov Sergey , Weber Mads C. , Schiemer Jason , Nataf Guillaume F. , Kreisel Jens
Published: June 2016

Polar tweed was discovered in mechanically stressed LaAlO3. Local patches of strained material (diameter ca. 5 μm) form interwoven patterns seen in birefringence images, Piezo-Force Microscopy (PFM) and Resonant Piezoelectric Spectroscopy (RPS). PFM and RPS observations prove unequivocally that electrical polarity exists inside the tweed patterns of LaAlO3. The local piezoelectric effect varies greatly within the tweed patterns and reaches magnitudes similar to quartz. The patterns were mapped by the shift of the Eg soft-mode frequency by Raman spectroscopy.
Insights on dramatic radial fluctuations in track formation by energetic ions
Authors: Sachan Ritesh , Zarkadoula Eva , Lang Maik , Trautmann Christina , Zhang Yanwen , Chisholm Matthew F. , Weber William J.
Published: June 2016

We report on unexpected dramatic radial variations in ion tracks formed by irradiation with energetic ions (2.3 GeV 208Pb) at a constant electronic energy-loss (~42 keV/nm) in pyrochlore-structured Gd2TiZrO7. Though previous studies have shown track formation and average track diameter measurements in the Gd2TixZr(1−x)O7 system, the present work clearly reveals the importance of the recrystallization process in ion track formation in this system, which leads to more morphological complexities in tracks than currently accepted behavior. The ion track profile is usually considered to be diametrically uniform for a constant value of electronic energy-loss. This study reveals the diameter variations to be as large as ~40% within an extremely short incremental track length of ~20 nm. Our molecular dynamics simulations show that these fluctuations in diameter of amorphous core and overall track diameter are attributed to the partial substitution of Ti atoms by Zr atoms, which have a large difference in ionic radii, on the B-site in pyrochlore lattice. This random distribution of Ti and Zr atoms leads to a local competition between amorphous phase formation (favored by Ti atoms) and defect-fluorite phase formation (favored by Zr atoms) during the recrystallization process and finally introduces large radial variations in track morphology.
Rethinking spontaneous giving: Extreme time pressure and ego-depletion favor self-regarding reactions
Authors: Capraro Valerio , Cococcioni Giorgia
Published: June 2016

Previous experimental studies suggest that cooperation in one-shot anonymous interactions is, on average, spontaneous, rather than calculative. To explain this finding, it has been proposed that people internalize cooperative heuristics in their everyday life and bring them as intuitive strategies in new and atypical situations. Yet, these studies have important limitations, as they promote intuitive responses usingweaktime pressure or conceptual priming of intuition. Since these manipulations do not deplete participants’ ability to reason completely, it remains unclear whether cooperative heuristics are really automatic or they emerge after a small, but positive, amount of deliberation. Consistent with the latter hypothesis, we report two experiments demonstrating that spontaneous reactions in one-shot anonymous interactions tend to be egoistic. In doing so, our findings shed further light on the cognitive underpinnings of cooperation, as they suggest that cooperation in one-shot interactions is not automatic, but appears only at later stages of reasoning.
4D printing smart biomedical scaffolds with novel soybean oil epoxidized acrylate
Authors: Miao Shida , Zhu Wei , Castro Nathan J. , Nowicki Margaret , Zhou Xuan , Cui Haitao , Fisher John P. , Zhang Lijie Grace
Published: June 2016

Photocurable, biocompatible liquid resins are highly desired for 3D stereolithography based bioprinting. Here we solidified a novel renewable soybean oil epoxidized acrylate, using a 3D laser printing technique, into smart and highly biocompatible scaffolds capable of supporting growth of multipotent human bone marrow mesenchymal stem cells (hMSCs). Porous scaffolds were readily fabricated by simply adjusting the printer infill density; superficial structures of the polymerized soybean oil epoxidized acrylate were significantly affected by laser frequency and printing speed. Shape memory tests confirmed that the scaffold fixed a temporary shape at −18 °C and fully recovered its original shape at human body temperature (37 °C), which indicated the great potential for 4D printing applications. Cytotoxicity analysis proved that the printed scaffolds had significant higher hMSC adhesion and proliferation than traditional polyethylene glycol diacrylate (PEGDA) and had no statistical difference from poly lactic acid (PLA) and polycaprolactone (PCL). This research is believed to significantly advance the development of biomedical scaffolds with renewable plant oils and advanced 3D fabrication techniques.
Withaferin A protects against palmitic acid-induced endothelial insulin resistance and dysfunction through suppression of oxidative stress and inflammation
Authors: Batumalaie Kalaivani , Amin Muhammad Arif , Murugan Dharmani Devi , Sattar Munavvar Zubaid Abdul , Abdullah Nor Azizan
Published: June 2016

Activation of inflammatory pathways via reactive oxygen species (ROS) by free fatty acids (FFA) in obesity gives rise to insulin resistance and endothelial dysfunction. Withaferin A (WA), possesses both antioxidant and anti-inflammatory properties and therefore would be a good strategy to suppress palmitic acid (PA)-induced oxidative stress and inflammation and hence, insulin resistance and dysfunction in the endothelium. Effect of WA on PA-induced insulin resistance in human umbilical vein endothelial cells (HUVECs) was determined by evaluating insulin signaling mechanisms whilst effect of this drug on PA-induced endothelial dysfunction was determined in acetylcholine-mediated relaxation in isolated rat aortic preparations. WA significantly inhibited ROS production and inflammation induced by PA. Furthermore, WA significantly decreased TNF-α and IL-6 production in endothelial cells by specifically suppressing IKKβ/NF-κβ phosphorylation. WA inhibited inflammation-stimulated IRS-1 serine phosphorylation and improved the impaired insulin PI3-K signaling and restored the decreased nitric oxide (NO) production triggered by PA. WA also decreased endothelin-1 and plasminogen activator inhibitor type-1 levels and restored the impaired endothelium-mediated vasodilation in isolated aortic preparations. These findings suggest that WA inhibited both ROS production and inflammation to restore impaired insulin resistance in cultured endothelial cells and improve endothelial dysfunction in rat aortic rings.
Apoptosis-mediated endothelial toxicity but not direct calcification or functional changes in anti-calcification proteins defines pathogenic effects of calcium phosphate bions
Authors: Kutikhin Anton G. , Velikanova Elena A. , Mukhamadiyarov Rinat A. , Glushkova Tatiana V. , Borisov Vadim V. , Matveeva Vera G. , Antonova Larisa V. , Filip’ev Dmitriy E. , Golovkin Alexey S. , Shishkova Daria K. , Burago Andrey Yu. , Frolov Alexey V. , Dolgov Viktor Yu. , Efimova Olga S. , Popova Anna N. , Malysheva Valentina Yu. , Vladimirov Alexandr A. , Sozinov Sergey A. , Ismagilov Zinfer R. , Russakov Dmitriy M. , Lomzov Alexander A. , Pyshnyi Dmitriy V. , Gutakovsky Anton K. , Zhivodkov Yuriy A. , Demidov Evgeniy A. , Peltek Sergey E. , Dolganyuk Viatcheslav F. , Babich Olga O. , Grigoriev Evgeniy V. , Brusina Elena B. , Barbarash Olga L. , Yuzhalin Arseniy E.
Published: June 2016

Calcium phosphate bions (CPB) are biomimetic mineralo-organic nanoparticles which represent a physiological mechanism regulating the function, transport and disposal of calcium and phosphorus in the human body. We hypothesised that CPB may be pathogenic entities and even a cause of cardiovascular calcification. Here we revealed that CPB isolated from calcified atherosclerotic plaques and artificially synthesised CPB are morphologically and chemically indistinguishable entities. Their formation is accelerated along with the increase in calcium salts-phosphates/serum concentration ratio. Experiments in vitro and in vivo showed that pathogenic effects of CPB are defined by apoptosis-mediated endothelial toxicity but not by direct tissue calcification or functional changes in anti-calcification proteins. Since the factors underlying the formation of CPB and their pathogenic mechanism closely resemble those responsible for atherosclerosis development, further research in this direction may help us to uncover triggers of this disease.
Antimicrobial activity, improved cell selectivity and mode of action of short PMAP-36-derived peptides against bacteria and Candida
Authors: Lyu Yinfeng , Yang Yang , Lyu Xiting , Dong Na , Shan Anshan
Published: June 2016

Antimicrobial peptides (AMPs) have recently attracted a great deal of attention as promising antibiotic candidates, but some obstacles such as toxicity and high synthesis cost must be addressed before developing them further. For developing short peptides with improved cell selectivity, we designed a series of modified PMAP-36 analogues. Antimicrobial assays showed that decreasing chain length in a certain range retained the high antimicrobial activity of the parental peptide and reduced hemolysis. The 18-mer peptide RI18 exhibited excellent antimicrobial activity against both bacteria and fungi, and its hemolytic activity was observably lower than PMAP-36 and melittin. The selectivity indexes of RI18 against bacteria and fungi were improved approximately 19-fold and 108-fold, respectively, compared to PMAP-36. In addition, serum did not affect the antibacterial activity of RI18 against E. coli but inhibited the antifungal efficiency against C. albicans. Flow cytometry and electron microscopy observation revealed that RI18 killed microbial cells primarily by damaging membrane integrity, leading to whole cell lysis. Taken together, these results suggest that RI18 has potential for further therapeutic research against frequently-encountered bacteria and fungi. Meanwhile, modification of AMPs is a promising strategy for developing novel antimicrobials to overcome drug-resistance.
Activation of liver X receptor inhibits the development of pulmonary carcinomas induced by 3-methylcholanthrene and butylated hydroxytoluene in BALB/c mice
Authors: Wang Qixue , Sun Lei , Yang Xiaoxiao , Ma Xingzhe , Li Qi , Chen Yuanli , Liu Ying , Zhang Di , Li Xiaoju , Xiang Rong , Wei Yuquan , Han Jihong , Duan Yajun
Published: June 2016

We previously reported that LXR ligand, T0901317, inhibited the growth of inoculated Lewis lung carcinoma in C57BL/6 mice by activating IFN-γ production. However, the effects of T0901317 on carcinogen-induced pulmonary carcinomas remain unknown. In this study, we initially conducted a statistical analysis on the data of human lung cancer samples extracted from the TCGA database and determined that survival rate/time of lung cancer patients and grade of lung adenocarcinoma were positively and negatively related to lung IFN-γ levels, respectively. We then determined the inhibitory effects of T0901317 on mouse pulmonary carcinomas induced by 3-methylcholanthrene (MCA) and butylated hydroxytoluene (BHT) or urethane. We found that T0901317 reduced morbidity and mortality in MCA/BHT-injected BALB/c mice by inhibiting lung adenocarcinoma. T0901317 also protected C57BL/6 mice, but not IFN-γ deficient (IFN-γ−/−, C57BL/6 background) mice, against MCA/BHT-induced lung hyperplasia/inflammation. In addition, we determined that T0901317 inhibited urethane-induced lung tumors in BABL/c mice. Furthermore, we determined that T0901317 prevented metastasis of 4T1 breast cancer cells in BALB/c mice. Administration of T0901317 substantially increased serum IFN-γ levels and lung IFN-γ expression in BABL/c and C57BL/6 mice. Taken together, our study demonstrates that LXR inhibits MCA/BHT-induced pulmonary carcinomas in BABL/c mice and the inhibition is associated with induction of IFN-γ production.