bluuebunny/crossref_metadata_2025 huggingface dataset:
A Human Periodontal Ligament Fibroblast Cell Line as a New Model to Study Periodontal Stress
Authors: Weider Matthias , Schröder Agnes , Docheva Denitsa , Rodrian Gabriele , Enderle Isabel , Seidel Corinna Lesley , Andreev Darja , Wegner Michael , Bozec Aline , Deschner James , Kirschneck Christian , Proff Peter , Gölz Lina
Published: October 2020

The periodontal ligament (PDL) is exposed to different kinds of mechanical stresses such as bite force or orthodontic tooth movement. A simple and efficient model to study molecular responses to mechanical stress is the application of compressive force onto primary human periodontal ligament fibroblasts via glass disks. Yet, this model suffers from the need for primary cells from human donors which have a limited proliferative capacity. Here we show that an immortalized cell line, PDL-hTERT, derived from primary human periodontal ligament fibroblasts exhibits characteristic responses to glass disk-mediated compressive force resembling those of primary cells. These responses include induction and secretion of pro-inflammatory markers, changes in expression of extracellular matrix-reorganizing genes and induction of genes related to angiogenesis, osteoblastogenesis and osteoclastogenesis. The fact that PDL-hTERT cells can easily be transfected broadens their usefulness, as molecular gain- and loss-of-function studies become feasible.
Tracing Autism Traits in Large Multiplex Families to Identify Endophenotypes of the Broader Autism Phenotype
Authors: Trevis Krysta J. , Brown Natasha J. , Green Cherie C. , Lockhart Paul J. , Desai Tarishi , Vick Tanya , Anderson Vicki , Pua Emmanuel P. K. , Bahlo Melanie , Delatycki Martin B. , Scheffer Ingrid E. , Wilson Sarah J.
Published: October 2020

Families comprising many individuals with Autism Spectrum Disorders (ASD) may carry a dominant predisposing mutation. We implemented rigorous phenotyping of the “Broader Autism Phenotype” (BAP) in large multiplex ASD families using a novel endophenotype approach for the identification and characterisation of distinct BAP endophenotypes. We evaluated ASD/BAP features using standardised tests and a semi-structured interview to assess social, intellectual, executive and adaptive functioning in 110 individuals, including two large multiplex families (Family A: 30; Family B: 35) and an independent sample of small families (n = 45). Our protocol identified four distinct psychological endophenotypes of the BAP that were evident across these independent samples, and showed high sensitivity (97%) and specificity (82%) for individuals classified with the BAP. Patterns of inheritance of identified endophenotypes varied between the two large multiplex families, supporting their utility for identifying genes in ASD.
Cocaine Administration and Its Abstinence Conditions Modulate Neuroglia
Authors: Gawlińska Kinga , Frankowska Małgorzata , Gawliński Dawid , Piechota Marcin , Korostyński Michał , Filip Małgorzata
Published: October 2020

Cocaine induces neuronal changes as well as non-neuronal (astrocytes, microglia, oligodendroglia) mechanisms, but these changes can also be modulated by various types of drug abstinence. Due to the very complex and still incompletely understood nature of cocaine use disorder, understanding of the mechanisms involved in addictive behavior is necessary to further search for effective pharmacotherapy of this disease. The aim of this study was to investigate changes at the gene and protein levels associated with glial cell activity after cocaine exposure, as well as during early cocaine abstinence (3 days) with extinction training or in home cage isolation. Cocaine self-administration significantly decreased myelin regulatory factor (MYRF) and cyclic nucleotide phosphodiesterase (CNP) expression in the hippocampus as well as pleckstrin (PLEK) and T-lymphocyte activation antigen (CD86) in the rat striatum. Depending on cocaine abstinence conditions, microglial PLEK expression was increased through extinction training but did not change in the home cage isolation. In addition, downregulation of gene expression associated with oligodendrocytes (CNP, MYRF) and microglia regulator of G protein signaling 1 (RGS1) was observed in the hippocampus, regardless of the type of drug abstinence, while downregulation of myelin and lymphocyte protein (MAL) expression was found only in rats exposed to abstinence in the home cage. Taken together, the presented results strongly suggest that cocaine abstinence evokes significant changes in gene expression associated with the proper functioning of glial cells, suggesting their significant involvement in adaptive changes in the brain associated with cocaine exposure. Interestingly, drug abstinence conditions are important factors influencing observed changes at the transcript levels of selected genes, which may be of clinical interest.
Comparative Study of Antennal Sensilla of Corixidae and Micronectidae (Hemiptera: Heteroptera: Nepomorpha: Corixoidea)
Authors: Nowińska Agnieszka , Chen Ping-ping , Brożek Jolanta
Published: October 2020

The goal of this study was to analyze the types and distributional patterns of sensilla in Corixoidea, which is part of the approach to the phylogeny study of Nepomorpha, based on the morphological characters of sensilla. This paper presents the results of the study, with the use of a scanning electron microscope (SEM), on the antennae of species from the families Corixidae and Micronectidae. The antennal sensilla of eleven species from Corixidae and two species from Micronectidae were studied. Five main types of sensilla with several subtypes of sensilla trichodea were found and described. The study has shown that the family Corixidae has a strong uniformity when it comes to antennal sensilla (similar patterns of sensilla trichodea and basiconica), and a similarity to the types and distributions of sensilla in two species of the family Micronectidae. However, significant differences between the families were also discovered (differences in sensilla presence on the first and second antennomeres, lack of sensilla coeloconica on the third antennomere in Micronectidae), which leads to a supportive conclusion of the systematic position of Micronectidae as a family.
Emphasizing Task-Specific Hypertrophy to Enhance Sequential Strength and Power Performance
Authors: Travis S. Kyle , Ishida Ai , Taber Christopher B. , Fry Andrew C. , Stone Michael H.
Published: October 2020

While strength is indeed a skill, most discussions have primarily considered structural adaptations rather than ultrastructural augmentation to improve performance. Altering the structural component of the muscle is often the aim of hypertrophic training, yet not all hypertrophy is equal; such alterations are dependent upon how the muscle adapts to the training stimuli and overall training stress. When comparing bodybuilders to strength and power athletes such as powerlifters, weightlifters, and throwers, while muscle size may be similar, the ability to produce force and power is often inequivalent. Thus, performance differences go beyond structural changes and may be due to the muscle’s ultrastructural constituents and training induced adaptations. Relative to potentiating strength and power performances, eliciting specific ultrastructural changes should be a variable of interest during hypertrophic training phases. By focusing on task-specific hypertrophy, it may be possible to achieve an optimal amount of hypertrophy while deemphasizing metabolic and aerobic components that are often associated with high-volume training. Therefore, the purpose of this article is to briefly address different types of hypertrophy and provide directions for practitioners who are aiming to achieve optimal rather than maximal hypertrophy, as it relates to altering ultrastructural muscular components, to potentiate strength and power performance.
Protective Efficacy of Lectin-Fc(IgG) Fusion Proteins In Vitro and in a Pulmonary Aspergillosis In Vivo Model
Authors: Rodriguez-de la Noval Claudia , Ruiz Mendoza Susana , de Souza Gonçalves Diego , da Silva Ferreira Marina , Honorato Leandro , Peralta José Mauro , Nimrichter Leonardo , Guimarães Allan J.
Published: October 2020

Aspergillosis cases by Aspergillus fumigatus have increased, along with fungal resistance to antifungals, urging the development of new therapies. Passive immunization targeting common fungal antigens, such as chitin and β-glucans, are promising and would eliminate the need of species-level diagnosis, thereby expediting the therapeutic intervention. However, these polysaccharides are poorly immunogenic. To overcome this drawback, we developed the lectin-Fc(IgG) fusion proteins, Dectin1-Fc(IgG2a), Dectin1-Fc(IgG2b) and wheat germ agglutinin (WGA)-Fc(IgG2a), based on their affinity to β-1,3-glucan and chitooligomers, respectively. The WGA-Fc(IgG2a) previously demonstrated antifungal activity against Histoplasma capsulatum, Cryptococcus neoformans and Candida albicans. In the present work, we evaluated the antifungal properties of these lectin-Fc(s) against A. fumigatus. Lectin-Fc(IgG)(s) bound in a dose-dependent manner to germinating conidia and this binding increased upon conidia germination. Both lectin-Fc(IgG)(s) displayed in vitro antifungal effects, such as inhibition of conidia germination, a reduced length of germ tubes and a diminished biofilm formation. Lectin-Fc(IgG)(s) also enhanced complement deposition on conidia and macrophage effector functions, such as increased phagocytosis and killing of fungi. Finally, administration of the Dectin-1-Fc(IgG2b) and WGA-Fc(IgG2a) protected mice infected with A. fumigatus, with a 20% survival and a doubled life-span of the infected mice, which was correlated to a fungal burden reduction in lungs and brains of treated animals. These results confirm the potential of lectin-Fc(IgGs)(s) as a broad-spectrum antifungal therapeutic.
Contaminated Land by Wildfire Effect on Ultramafic Soil and Associated Human Health and Ecological Risk
Authors: Alexakis Dimitrios E.
Published: October 2020

The purpose of this study is the evaluation of fire effect on contaminated land and the assessment of the associated risk of human health and terrestrial ecological receptors. Ash and soil samples were gathered from burned and unburned areas (central Evia, Greece) which are adjacent with a Natura 2000 area. The geochemical dataset includes 20 sampling sites and 35 elements. The wildfire severity was investigated by applying a macroscopic approach and field observations. Statistical and spatial analysis were applied for delineating the distribution of elements in ash and soil. Elemental balance approach was performed for estimating net gain (+) or loss (−) to the ash. Element contents in sampling sites were compared to screening values proposed by the literature. Hundreds of hectares of burned land including wildland areas in central Evia are contaminated with (contents in mg Kg−1), Co (up to 43.5), Cr (up to 244), Mn (up to 1158), Ni (up to 463) associated with geogenic sources such as serpentinite peridotites and Ni-laterite deposits. Aluminum, As, Cd, Co, Cr, Fe, Mn, Ni, Pb, V and Zn contents recorded in the sampling sites are posing a potential risk to human health and ecological receptors.
Protective Effects of Oenothera biennis against Hydrogen Peroxide-Induced Oxidative Stress and Cell Death in Skin Keratinocytes
Authors: Lee Seung , Kim Chul , Hwang Buyng , Choi Kyung-Min , Yang In-Jun , Kim Gi-Young , Choi Yung , Park Cheol , Jeong Jin-Woo
Published: October 2020

Background: Oenothera biennis (evening primrose) produces bioactive substances with a diverse range of pharmacological functions. However, it is currently unknown whether extract prepared from the aerial parts of O. biennis (APOB) can protect the skin against oxidative stress. Objective: The aim of this study is to investigate the protective effects of APOB against oxidative stress-induced damage in human skin keratinocytes (HaCaT) and elucidate the underlying mechanisms. Methods: We pretreated HaCaT cells with various concentrations of APOB or the antioxidant N-acetyl-L-cysteine before applying H2O2. We then compared the cell viability, intracellular reactive oxygen species (ROS) production, and DNA and mitochondrial damage between pretreated and untreated control cells using a range of assays, flow cytometry, and Western blot analysis and also examined the reducing power and DPPH free radical scavenging activity of APOB. Results: APOB pretreatment significantly increased cell viability, effectively attenuated H2O2-induced comet tail formation, and inhibited H2O2-induced phosphorylation of the histone γH2AX, as well as the number of apoptotic bodies and Annexin V-positive cells. APOB was found to have high reducing power and DPPH radical scavenging activity and also exhibited scavenging activity against intracellular ROS accumulation and restored the loss of mitochondrial membrane potential caused by H2O2. APOB pretreatment almost totally reversed the enhanced cleavage of caspase-3, the degradation of poly (ADP-ribose)-polymerase (PARP), DNA fragmentation that usually occurs in the presence of H2O2, and increased the levels of heme oxygenase-1 (HO-1), a potent antioxidant enzyme that is associated with the induction of nuclear factor-erythroid 2-related factor 2 (Nrf2). Conclusions: APOB can protect HaCaT cells from H2O2-induced DNA damage and cell death by blocking cellular damage related to oxidative stress via a mechanism that affects ROS elimination and by activating the Nrf2/HO-1 signaling pathway.
3D Printed Model of Extrahepatic Biliary Ducts for Biliary Stent Testing
Authors: Thomas Joanna , Patel Sagar , Troop Leia , Guru Robyn , Faist Nicholas , Bellott Brian J. , Esterlen Bethany A.
Published: October 2020

Several inflammatory conditions of the bile ducts cause strictures that prevent the drainage of bile into the gastrointestinal tract. Non-pharmacological treatments to re-establish bile flow include plastic or self-expanding metal stents (SEMs) that are inserted in the bile ducts during endoscopic retrograde cholangiopancreatography (ERCP) procedures. The focus of this study was to 3D print an anatomically accurate model of the extrahepatic bile ducts (EHBDs) with tissue-like mechanical properties to improve in vitro testing of stent prototypes. Following generation of an EHBD model via computer aided design (CAD), we tested the ability of Formlabs SLA 3D printers to precisely print the model with polymers selected based on the desired mechanical properties. We found the printers were reliable in printing the dimensionally accurate EHBD model with candidate polymers. Next, we evaluated the mechanical properties of Formlabs Elastic (FE), Flexible (FF), and Durable (FD) resins pre- and post-exposure to water, saline, or bile acid solution at 37 °C for up to one week. FE possessed the most bile duct-like mechanical properties based on its elastic moduli, percent elongations at break, and changes in mass under all liquid exposure conditions. EHBD models printed in FE sustained no functional damage during biliary stent deployment or when tube connectors were inserted, and provided a high level of visualization of deployed stents. These results demonstrate that our 3D printed EHBD model facilitates more realistic pre-clinical in vitro testing of biliary stent prototypes.
Mesenchymal Stromal Cell Therapy in the Management of Perianal Fistulas in Crohn’s Disease: An Up-To-Date Review
Authors: Gallo Gaetano , Tiesi Vincenzo , Fulginiti Serena , De Paola Gilda , Vescio Giuseppina , Sammarco Giuseppe
Published: October 2020

Crohn’s Disease (CD) is a chronic inflammatory disorder that potentially involves the entire gastrointestinal tract. Perianal fistulizing CD (pCD) is a serious and frequent complication associated with significant morbidities and a heavy negative impact on quality of life. The aim of CD treatment is to induce and maintain disease remission and to promote mucosal repair. Unfortunately, even the best therapeutic regimens in pCD do not have long-term efficacy and cause a significant number of side effects. Therefore, it is mandatory to study new therapeutical options such as the use of mesenchymal stromal cells (MSCs). These cells promote tissue repair via the induction of immunomodulation. The present review aims to analyze the existing updated scientific literature on MSCs adoption in the treatment of pCD to evaluate its efficacy and safety and to compare the use of bone marrow and adipose tissue derived MSCs, type of administration, and dose required for recovery.
Long-Term Efficacy of Variable-Thread Tapered Implants—A Retrospective, Clinical and Radiological Evaluation
Authors: Blume Oliver , Schnödt Eva Maria , Back Michael , Wildenhof Jan IR , Probst Florian A. , Otto Sven
Published: October 2020

Background and Objective: There is multifaceted evidence that variable-thread tapered implants (VTTIs) offer high primary stability but few regarding the long-term success. This retrospective clinical and radiological cohort study assessed the long-term outcome of VTTIs. Material and Methods: All patients treated in an OMFS practice with NobelActive Internal® VTTI between October 2007 and September 2011 were invited for clinical examination. The outcome variables were (i) survival rate, (ii) implant success according to the “Health Scale for Dental Implants” and (iii) prevalence of peri-implantitis. Furthermore, the effect of local and systemic risk factors was investigated. Results: In 81 subjects (46 females and 35 males, mean age 65.6 years) 270 implants (157 VTTIs and 113 others as a control group) were analyzed. In 7 out of 81 patients (8.6%), 8 out of 157 VTTIs (5.1%) and 5 out of 113 other implants (4.4%) were lost. Peri-implantitis, defined as (i) presence of bleeding on gentle probing (0.25 N) or exudation and (ii) radiographic bone loss exceeding 0.5 mm since implant insertion to last follow-up, was the most common reason for implant loss (11 out of 13, 84.6%). Sixty-six out of 87 VTTIs (75.9%) were successful. Seventeen out of 42 patients (40.5%) developed peri-implantitis on 29 out of 79 VTTI sites (36.7%). Plaque and missing keratinized peri-implant mucosa were identified as potential risk factors for the development of peri-implantitis. Conclusion: Variable-thread tapered implants showed good long-term results, even in low bone quality. Peri-implantitis was the most common reason for implant failure and may be connected to certain risk factors.
Fabrication of Magnesium–NiTip Composites via Friction Stir Processing: Effect of Tool Profile
Authors: Gangil N. , Nagar H. , Mohammed S.M.A.K. , Singh D. , Siddiquee A.N. , Maheshwari S. , Chen D.L.
Published: October 2020

In this study, a solid-state fabrication route via friction stir processing (FSP) was used to fabricate Nitinol particulate (NiTip)-reinforced magnesium-based composites to avoid the diffusion reaction and the formation of brittle interfacial compounds. The effect of four tool profiles on the homogeneity in the dispersion of NiTip particles in the magnesium matrix and microhardness was examined and analyzed. A counter-clockwise scrolled shoulder with a plain cylindrical pin and three tools with a flat shoulder having plain cylindrical pin, left-hand, and right-hand threaded pins were used and compared. The tool profiles were observed to exhibit a significant influence on the microstructure of the fabricated Mg/NiTip composites. A wider and more uniform distribution of NiTip particles along with superior bonding with magnesium matrix was achieved with a left-hand threaded cylindrical pin tool. The incorporation of NiTip gave rise to a significant increase in the microhardness of the fabricated composites due to a variety of strengthening mechanisms.
Leaching of Pure Chalcocite with Reject Brine and MnO2 from Manganese Nodules
Authors: Torres David , Trigueros Emilio , Robles Pedro , Leiva Williams H. , Jeldres Ricardo I. , Toledo Pedro G. , Toro Norman
Published: October 2020

Chalcocite (Cu2S) has the fastest kinetics of dissolution of Cu in chlorinated media of all copper sulfide minerals. Chalcocite has been identified as having economic interest due to its abundance, although the water necessary for its dissolution is scarce in many regions. In this work, the replacement of fresh water by sea water or by reject brine with high chloride content from desalination plants is analyzed. Additionally, the effect of adding MnO2 from available manganese nodules in vast quantities at the bottom of the sea is studied. Reject brine shows better results than sea water, and the addition of MnO2 to the brine significantly increases the kinetics of chalcocite dissolution in a short time. H2SO4 concentration is found to be irrelevant when working at high concentrations of chloride and MnO2. The best results, 71% Cu extractions in 48 h, are obtained for reject brine, 100 mg of MnO2 per 200 g of mineral and H2SO4 0.5 mol/L. The results are expected to contribute to a sustainable process of dissolution of chalcocite by using the reject brine from desalination plants.
Transcriptomic Analysis of Shiga Toxin-Producing Escherichia coli during Initial Contact with Cattle Colonic Explants
Authors: Stromberg Zachary R. , Masonbrink Rick E. , Mellata Melha
Published: October 2020

Foodborne pathogens are a public health threat globally. Shiga toxin-producing Escherichia coli (STEC), particularly O26, O111, and O157 STEC, are often associated with foodborne illness in humans. To create effective preharvest interventions, it is critical to understand which factors STEC strains use to colonize the gastrointestinal tract of cattle, which serves as the reservoir for these pathogens. Several colonization factors are known, but little is understood about initial STEC colonization factors. Our objective was to identify these factors via contrasting gene expression between nonpathogenic E. coli and STEC. Colonic explants were inoculated with nonpathogenic E. coli strain MG1655 or STEC strains (O26, O111, or O157), bacterial colonization levels were determined, and RNA was isolated and sequenced. STEC strains adhered to colonic explants at numerically but not significantly higher levels compared to MG1655. After incubation with colonic explants, flagellin (fliC) was upregulated (log2 fold-change = 4.0, p < 0.0001) in O157 STEC, and collectively, Lon protease (lon) was upregulated (log2 fold-change = 3.6, p = 0.0009) in STEC strains compared to MG1655. These results demonstrate that H7 flagellum and Lon protease may play roles in early colonization and could be potential targets to reduce colonization in cattle.
Uncovering Unique Green Algae and Cyanobacteria Isolated from Biocrusts in Highly Saline Potash Tailing Pile Habitats, Using an Integrative Approach
Authors: Sommer Veronika , Mikhailyuk Tatiana , Glaser Karin , Karsten Ulf
Published: October 2020

Potash tailing piles caused by fertilizer production shape their surroundings because of the associated salt impact. A previous study in these environments addressed the functional community “biocrust” comprising various micro- and macro-organisms inhabiting the soil surface. In that previous study, biocrust microalgae and cyanobacteria were isolated and morphologically identified amongst an ecological discussion. However, morphological species identification maybe is difficult because of phenotypic plasticity, which might lead to misidentifications. The present study revisited the earlier species list using an integrative approach, including molecular methods. Seventy-six strains were sequenced using the markers small subunit (SSU) rRNA gene and internal transcribed spacer (ITS). Phylogenetic analyses confirmed some morphologically identified species. However, several other strains could only be identified at the genus level. This indicates a high proportion of possibly unknown taxa, underlined by the low congruence of the previous morphological identifications to our results. In general, the integrative approach resulted in more precise species identifications and should be considered as an extension of the previous morphological species list. The majority of taxa found were common in saline habitats, whereas some were more likely to occur in nonsaline environments. Consequently, biocrusts in saline environments of potash tailing piles contain unique microalgae and cyanobacteria that will possibly reveal several new taxa in more detailed future studies and, hence, provide new data on the biodiversity, as well as new candidates for applied research.
Effective Detection of Nafion®-Based Theranostic Nanocapsules Through 19F Ultra-Short Echo Time MRI
Authors: Łopuszyńska Natalia , Szczepanowicz Krzysztof , Jasiński Krzysztof , Warszyński Piotr , Węglarz Władysław P.
Published: October 2020

The application of the Three-Dimensional Ultra-Short Echo Time (3D UTE)pulse sequence at a high magnetic field for visualization of the distribution of 19F loaded theranostic core-shell nanocapsules with Nafion® (1,1,2,2-tetrafluoroethene; 1,1,2,2-tetrafluoro-2- [1,1,1,2,3,3-hexafluoro-3-(1,2,2-trifluoroethenoxy)propan-2-yl] oxyethanesulfonic acid) incorporated into the shell is presented. The nanocarriers were formed via the layer-by-layer technique with biodegradable polyelectrolytes: PLL (Poly-L-lysine), and with Nafion®: polymer with high 19F content. Before imaging, an MR (magnetic resonance) spectroscopy and T1 and T2 measurements were performed, resulting in values of T2 between 1.3 ms and 3.0 ms, depending on the spectral line. To overcome limitations due to such short T2, the 3D UTE pulse sequence was applied for 19F MR imaging. First Nafion® solutions of various concentrations were measured to check the detection limit of our system for the investigated molecule. Next, the imaging of a phantom containing core-shell nanocapsules was performed to assess the possibility of visualizing their distribution in the samples. Images of Nafion® containing samples with SNR ≥ 5 with acquisition time below 30 min for 19F concentration as low as 1.53 × 10−2 mmol 19F/g of sample, were obtained. This is comparable with the results obtained for molecules, which exhibit more preferable MR characteristics.
Functional Sr0.5Ba0.5Sm0.02Fe11.98O4/x(Ni0.8Zn0.2Fe2O4) Hard–Soft Ferrite Nanocomposites: Structure, Magnetic and Microwave Properties
Authors: Algarou Norah A. , Slimani Yassine , Almessiere Munirah A. , Sadaqat Ali , Trukhanov Alex V. , Gondal Mohammad A. , Hakeem Abbas S. , Trukhanov Sergei V. , Vakhitov Maksim G. , Klygach Denis S. , Manikandan Ayyar , Baykal Abdulhadi
Published: October 2020

This paper reports the correlation between the composition of the functional Sr0.5Ba0.5Sm0.02Fe11.98O19/x(Ni0.8Zn0.2Fe2O4) hard–soft nanocomposites (SrBaSmFe/x(NiZnFe) NCs), where 0.0 ≤ x ≤ 3.0, and their structural features, magnetic, and microwave properties. SrBaSmFe/x(NiZnFe) hard/soft ferrite NCs are produced using the one-pot citrate combustion method. According to the XRD analysis, all samples showed the co-existence of both SrBaSmFe and NiZnFe phases in different ratios. Magnetic properties are measured in a wide range of magnetic fields and temperatures (10 and 300 K) and correlated well with the composition of the investigated samples. The microwave properties (frequency dispersions of the magnetic permeability, and electrical permittivity) are discussed by using the co-axial method in the frequency range of 0.7–18 GHz. Non-linear dependences of the main microwave features were observed with varying of composition. The microwave behavior correlated well with the composite theory. These results could be used in practice for developing antenna materials.
Chemical Composition and In Vitro Antiplasmodial Activity of the Ethanolic Extract of Cyperus articulatus var. nodosus Residue
Authors: Assis Francisco Flávio Vieira de , Silva Nazaré Carneiro da , Moraes Waldiney Pires , Barata Lauro Euclides Soares , Minervino Antonio Humberto Hamad
Published: October 2020

Cyperus articulatus L. is popularly known as priprioca. Its rhizomes are used as a medicine in the treatment of malaria in traditional medicine. Since priprioca oil is extracted for commercial purpose, we evaluated if the components from the priprioca residue can be a source of antiplasmodial active molecules. This study aimed to determine the in vitro antiplasmodial and cytotoxicity activities of the ethanolic extract of C. articulatus as an in vitro antiplasmodial agent. From the solid residue of the plant rhizomes, 40 g samples were removed and subjected to hot extraction using a Soxhlet extractor. The in vitro antiplasmodial activity was determined using the W2 and 3D7 strains of P. falciparum. The phytochemical study identified the following main compounds: corymbolone (14.25%), cyclocolorenone (9.75%), and cadalene (8.36%). The extract exhibited moderate IC50 (inhibitory concentration) against the two strains of P. falciparum: 1.21 ± 0.01 against the W2 strain and 1.10 ± 0.06 µg/mL against the 3D7 strain. Our results show the therapeutic potential of priprioca residue as a low-cost antiplasmodial agent.
Dangerous Liaisons: Interactions of Cryptococcus neoformans with Host Phagocytes
Authors: Gaylord Elizabeth A. , Choy Hau Lam , Doering Tamara L.
Published: October 2020

Cryptococcus neoformans is an opportunistic fungal pathogen and a leading cause of death in immunocompromised individuals. The interactions of this yeast with host phagocytes are critical to disease outcome, and C. neoformans is equipped with an array of factors to modulate these processes. Cryptococcal infection begins with the deposition of infectious particles into the lungs, where the fungal cells deploy various antiphagocytic factors to resist internalization by host cells. If the cryptococci are still engulfed, they can survive and proliferate within host cells by modulating the phagolysosome environment in which they reside. Lastly, cryptococcal cells may escape from phagocytes by host cell lysis, nonlytic exocytosis, or lateral cell-to-cell transfer. The interactions between C. neoformans and host phagocytes also influence the dissemination of this pathogen to the brain, where it may cross the blood-brain barrier and cause an often-fatal meningoencephalitis. In this review, we highlight key cryptococcal factors involved in various stages of cryptococcal-host interaction and pathogenesis.
Genotype-Specific Antioxidant Responses and Assessment of Resistance Against Sclerotinia sclerotiorum Causing Sclerotinia Rot in Indian Mustard
Authors: Singh Manjeet , Avtar Ram , Pal Ajay , Punia Rakesh , Singh Vivek K. , Bishnoi Mahavir , Singh Anoop , Choudhary Raju Ram , Mandhania Shiwani
Published: October 2020

Productivity of Indian mustard, an important oilseed crop of India, is affected by several pathogens. Among them, the hemibiotroph Sclerotinia sclerotiorum, which causes sclerotinia rot disease, is the most devastating fungal pathogen causing up to 90% yield losses. The availability of host resistance is the only efficient approach to control and understand the host–pathogen interaction. Therefore, the present investigation was carried out using six Indian mustard genotypes with contrasting behavior towards sclerotinia rot to study the antioxidant resistance mechanism against S. sclerotiorum. The plants at post-flowering stage were inoculated with five-day-old pure culture of S. sclerotiorum using artificial stem inoculation method. Disease evaluation revealed significant genotypic differences for mean lesion length among the tested genotypes, where genotype DRMR 2035 was found highly resistant, while genotypes RH 1569 and RH 1633 were found highly susceptible. The resistant genotypes had more phenolics and higher activities of peroxidase, catalase and polyphenol oxidase which provide them more efficient and strong antioxidant systems as compared with susceptible genotypes. Studies of antioxidative mechanisms validate the results of disease responses.
The Pink Tank in the Room: The Role of Religious Considerations in the Discussion of Women’s Combat Service—The Case of the Israel Defense Forces
Authors: Rosman Elisheva
Published: October 2020

Women serve in diverse roles in the 21st century militaries of the world. They are no longer banned from combat. The presence of women on the battlefield has raised religious arguments and considerations. What role do religious arguments play in the discussion regarding women’s military service? Using media, internal publications, as well as academic articles, the current paper examined this question in the context of the Israel Defense Forces (IDF): a conscription-based military that conscripts both men and women, religious and secular, for both combat and noncombat postings. Using the case of the pilot program in the IDF attempting to integrate women in the Israeli tank corps, as well as gauging the way religious men view this change, the paper argues that religious considerations serve the same purpose as functional considerations and can be amplified or lessened, as needed.
On the Trajectory Planning for Energy Efficiency in Industrial Robotic Systems †
Authors: Carabin Giovanni , Scalera Lorenzo
Published: October 2020

In this paper, we present an approach for the minimum-energy trajectory planning in industrial robotic systems. The method is based on the dynamic and electro-mechanical modeling of one-degree-of-freedom systems and the derivation of the energy formulation for standard point-to-point trajectories, as, for instance, trapezoidal and cycloidal speed profiles. The proposed approach is experimentally validated on two robotic systems, namely a linear axis of a Cartesian manipulator built in the 1990’s, and a test bench composed of two servomotors directly connected or coupled by means of a planetary gear. During the tests, the electrical power expended by the systems is measured and integrated over time to compute the energy consumption for each trajectory. Despite the limitations of the energy measurement systems, the results reveal a trend in agreement with the theoretical calculations, showing the possibility of applying the method for enhancing the performance of industrial robotic systems in terms of energy consumption in point-to-point motions.
Bismuth Subcarbonate Decorated Reduced Graphene Oxide Nanocomposite for the Sensitive Stripping Voltammetry Analysis of Pb(II) and Cd(II) in Water
Authors: Zhao Guo , Sedki Mohammed , Ma Shengcun , Villarreal Claudia , Mulchandani Ashok , Jassby David
Published: October 2020

In this paper, bismuth subcarbonate (BiO)2CO3-reduced graphene oxide nanocomposite incorporated in Nafion matrix ((BiO)2CO3-rGO-Nafion) was synthesized and further applied, for the first time, in the sensitive detection of Pb(II) and Cd(II) by square-wave anodic stripping voltammetry (SWASV). The as-synthesized nanocomposites were characterized by energy-dispersive spectroscopy (EDS), Raman spectroscopy, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). (BiO)2CO3 composite plays a key role in the improvement of the detection sensitivity, which can form multicomponent alloy with cadmium and lead. Additionally, the unique structure of rGO can enlarge the surface area and provide abundant active sites. Moreover, Nafion incorporation in the nanocomposite can effectively increase the adhesion and mechanical strength of the film, and further improve the preconcetration ability due to the cation-exchange capacity of its abundant sulfonate groups. As expected, the (BiO)2CO3-rGO/Nafion nanocomposite-modified glassy carbon electrode ((BiO)2CO3-rGO-Nafion/GCE) achieved low detection limits of 0.24 μg/L for Pb(II) and 0.16 μg/L for Cd(II), in the linear range of 1.0–60 μg/L, and showed some excellent performance, such as high stability, good selectivity, and sensitivity. Finally, synthetic water samples were prepared and further used to verify the practicability of the (BiO)2CO3-rGO-Nafion/GCE with satisfactory results.
Characterization and Miniaturization of Silver-Nanoparticle Microcoil via Aerosol Jet Printing Techniques for Micromagnetic Cochlear Stimulation
Authors: Sarreal Ressa Reneth , Bhatti Pamela
Published: October 2020

According to the National Institute of Deafness and other Communication Disorders 2012 report, the number of cochlear implant (CI) users is steadily increasing from 324,000 CI users worldwide. The cochlea, located in the inner ear, is a snail-like structure that exhibits a tonotopic geometry where acoustic waves are filtered spatially according to frequency. Throughout the cochlea, there exist hair cells that transduce sensed acoustic waves into an electrical signal that is carried by the auditory nerve to ultimately reach the auditory cortex of the brain. A cochlear implant bridges the gap if non-functional hair cells are present. Conventional CIs directly inject an electrical current into surrounding tissue via an implanted electrode array and exploit the frequency-to-place mapping of the cochlea. However, the current is dispersed in perilymph, a conductive bodily fluid within the cochlea, causing a spread of excitation. Magnetic fields are more impervious to the effects of the cochlear environment due to the material properties of perilymph and surrounding tissue, demonstrating potential to improve precision. As an alternative to conventional CI electrodes, the development and miniaturization of microcoils intended for micromagnetic stimulation of intracochlear neural elements is described. As a step toward realizing a microcoil array sized for cochlear implantation, human-sized coils were prototyped via aerosol jet printing. The batch reproducible aerosol jet printed microcoils have a diameter of 1800 μm, trace width and trace spacing of 112.5 μm, 12 μm thickness, and inductance values of approximately 15.5 nH. Modelling results indicate that the coils have a combined depolarization–hyperpolarization region that spans 1.5 mm and produce a more restrictive spread of activation when compared with conventional CI.
Design of a Multipurpose Photonic Chip Architecture for THz Dual-Comb Spectrometers
Authors: Betancur-Pérez Andrés , Martín-Mateos Pedro , Dios Cristina de , Acedo Pablo
Published: October 2020

In this work, we present a multipurpose photonic integrated circuit capable of generating multiheterodyne complex Dual-Combs (DC) THz signals. Our work focuses on translating the functionality of an electro-optic tunable DC system into a photonic chip employing standard building blocks to ensure the scalability and cost efficiency of the integrated device. The architecture we analyze for integration is based on three stages: a seed comb, a mode selection stage and a DC stage. This final DC stage includes a frequency shifter, a key element to improve the final detection of the THz signals and obtain real-time operation. This investigation covers three key aspects: (1) a solution for comb line selection on GHz spaced combs using OIL or OPLL on photonic chips is studied and evaluated, (2) a simple and versatile scheme to produce a frequency shift using the double sideband suppressed carrier modulation technique and an asymmetric Mach Zehnder Interferometer to filter one of the sidebands is proposed, and (3) a multipurpose architecture that can offer a versatile effective device, moving from application-specific PICs to general-purpose PICs. Using the building blocks (BBs) available from an InP-based foundry, we obtained simulations that offer a high-quality Dual-Comb frequency shifted signal with a side mode suppression ratio around 21 dB, and 41 dB after photodetection with an intermediate frequency of 1 MHz. We tested our system to generate a Dual-Comb with 10 kHz of frequency spacing and an OOK modulation with 5 Gbps which can be down-converted to the THz range by a square law detector. It is also important to note that the presented architecture is multipurpose and can also be applied to THz communications. This design is a step to enable a commercial THz photonic chip for multiple applications such as THz spectroscopy, THz multispectral imaging and THz telecommunications and offers the possibility of being fabricated in a multi-project wafer.
CdTe Based Energy Resolving, X-ray Photon Counting Detector Performance Assessment: The Effects of Charge Sharing Correction Algorithm Choice
Authors: Pickford Scienti Oliver L. P. , Bamber Jeffrey C. , Darambara Dimitra G.
Published: October 2020

Most modern energy resolving, photon counting detectors employ small (sub 1 mm) pixels for high spatial resolution and low per pixel count rate requirements. These small pixels can suffer from a range of charge sharing effects (CSEs) that degrade both spectral analysis and imaging metrics. A range of charge sharing correction algorithms (CSCAs) have been proposed and validated by different groups to reduce CSEs, however their performance is often compared solely to the same system when no such corrections are made. In this paper, a combination of Monte Carlo and finite element methods are used to compare six different CSCAs with the case where no CSCA is employed, with respect to four different metrics: absolute detection efficiency, photopeak detection efficiency, relative coincidence counts, and binned spectral efficiency. The performance of the various CSCAs is explored when running on systems with pixel pitches ranging from 100 µm to 600µm, in 50 µm increments, and fluxes from 106 to 108 photons mm−2 s−1 are considered. Novel mechanistic explanations for the difference in performance of the various CSCAs are proposed and supported. This work represents a subset of a larger project in which pixel pitch, thickness, flux, and CSCA are all varied systematically.
Enterprise Security for the Internet of Things (IoT): Lightweight Bootstrapping with EAP-NOOB
Authors: Peltonen Aleksi , Inglés Eduardo , Latvala Sampsa , Garcia-Carrillo Dan , Sethi Mohit , Aura Tuomas
Published: October 2020

The emergence of radio technologies, such as Zigbee, Z-Wave, and Bluetooth Mesh, has transformed simple physical devices into smart objects that can understand and react to their environment. Devices, such as light bulbs, door locks, and window blinds, can now be connected to, and remotely controlled from, the Internet. Given the resource-constrained nature of many of these devices, they have typically relied on the use of universal global shared secrets for the initial bootstrapping and commissioning phase. Such a scheme has obvious security weaknesses and it also creates undesirable walled-gardens where devices of one ecosystem do not inter-operate with the other. In this paper, we investigate whether the standard Extensible Authentication Protocol (EAP) framework can be used for secure bootstrapping of resource-constrained devices. EAP naturally provides the benefits of per-device individual credentials, straightforward revocation, and isolation of devices. In particular, we look at the Nimble out-of-band authentication for EAP (EAP-NOOB) as a candidate EAP authentication method. EAP-NOOB greatly simplifies deployment of such devices as it does not require them to be pre-provisioned with credentials of any sort. Based on our implementation experience on off-the-shelf hardware, we demonstrate that lightweight EAP-NOOB is indeed a way forward to securely bootstrap such devices.
Biometric Signals Estimation Using Single Photon Camera and Deep Learning
Authors: Paracchini Marco , Marcon Marco , Villa Federica , Zappa Franco , Tubaro Stefano
Published: October 2020

The problem of performing remote biomedical measurements using just a video stream of a subject face is called remote photoplethysmography (rPPG). The aim of this work is to propose a novel method able to perform rPPG using single-photon avalanche diode (SPAD) cameras. These are extremely accurate cameras able to detect even a single photon and are already used in many other applications. Moreover, a novel method that mixes deep learning and traditional signal analysis is proposed in order to extract and study the pulse signal. Experimental results show that this system achieves accurate results in the estimation of biomedical information such as heart rate, respiration rate, and tachogram. Lastly, thanks to the adoption of the deep learning segmentation method and dependability checks, this method could be adopted in non-ideal working conditions—for example, in the presence of partial facial occlusions.
Two-Hop Monitoring Mechanism Based on Relaxed Flow Conservation Constraints against Selective Routing Attacks in Wireless Sensor Networks
Authors: Derhab Abdelouahid , Bouras Abdelghani , Belaoued Mohamed , Maglaras Leandros , Khan Farrukh Aslam
Published: October 2020

In this paper, we investigate the problem of selective routing attack in wireless sensor networks by considering a novel threat, named the upstream-node effect, which limits the accuracy of the monitoring functions in deciding whether a monitored node is legitimate or malicious. To address this limitation, we propose a one-dimensional one-class classifier, named relaxed flow conservation constraint, as an intrusion detection scheme to counter the upstream node attack. Each node uses four types of relaxed flow conservation constraints to monitor all of its neighbors. Three constraints are applied by using one-hop knowledge, and the fourth one is calculated by monitoring two-hop information. The latter is obtained by proposing two-hop energy-efficient and secure reporting scheme. We theoretically analyze the security and performance of the proposed intrusion detection method. We also show the superiority of relaxed flow conservation constraint in defending against upstream node attack compared to other schemes. The simulation results show that the proposed intrusion detection system achieves good results in terms of detection effectiveness.
Experimental Evaluation of Machine Learning Methods for Robust Received Signal Strength-Based Visible Light Positioning
Authors: Raes Willem , Knudde Nicolas , De Bruycker Jorik , Dhaene Tom , Stevens Nobby
Published: October 2020

In this work, the use of Machine Learning methods for robust Received Signal Strength (RSS)-based Visible Light Positioning (VLP) is experimentally evaluated. The performance of Multilayer Perceptron (MLP) models and Gaussian processes (GP) is investigated when using relative RSS input features. The experimental set-up for the RSS-based VLP technology uses light-emitting diodes (LEDs) transmitting intensity modulated light and a single photodiode (PD) as a receiver. The experiments focus on achieving robustness to cope with unknown received signal strength modifications over time. Therefore, several datasets were collected, where per dataset either the LEDs transmitting power is modified or the PD aperture is partly obfuscated by dust particles. Two relative RSS schemes are investigated. The first scheme uses the maximum received light intensity to normalize the received RSS vector, while the second approach obtains RSS ratios by combining all possible unique pairs of received intensities. The Machine Learning (ML) methods are compared to a relative multilateration implementation. It is demonstrated that the adopted MLP and GP models exhibit superior performance and higher robustness when compared to the multilateration strategies. Furthermore, when comparing the investigated ML models, the GP model is proven to be more robust than the MLP for the considered scenarios.