bluuebunny/crossref_metadata_2025 huggingface dataset:
Courting Constitutionalism
Authors: Cheema Moeen , Dyzenhaus David , Poole Thomas
Published: December 2021

Over the last decade, the Supreme Court of Pakistan has emerged as a powerful and overtly political institution. While the strong form of judicial review adopted by the Supreme Court has fostered the perception of a sudden and ahistorical judicialisation of politics, the judiciary's prominent role in adjudicating issues of governance and statecraft was long in the making. This book presents a deeply contextualised account of law in Pakistan and situates the judicial review jurisprudence of the superior courts in the context of historical developments in constitutional politics, evolution of state structures and broader social transformations. This book highlights that the bedrock of judicial review has remained in administrative law; it is through the consistent development of the 'Writ jurisdiction' and the judicial review of administrative action that Pakistan's superior courts have progressively carved an expansive institutional role and aggrandised themselves to the status of the regulator of the state.
Mind and World in Aristotle's De Anima
Authors: Kelsey Sean
Published: December 2021

Why is the human mind able to perceive and understand the truth about reality; that is, why does it seem to be the mind's specific function to know the world? Sean Kelsey argues that both the question itself and the way Aristotle answers it are key to understanding his work De Anima, a systematic philosophical account of the soul and its powers. In this original reading of a familiar but highly compressed text, Kelsey shows how this question underpins Aristotle's inquiry into the nature of soul, sensibility, and intelligence. He argues that, for Aristotle, the reason why it is in human nature to know beings is that 'the soul in a way is all beings'. This new perspective on the De Anima throws fresh and interesting light on familiar Aristotelian doctrines: for example, that sensibility is a kind of ratio (logos), or that the intellect is simple, separate, and unmixed.
Digital Entrepreneurship
Authors: Morabito Vincenzo
Published: December 2021

A comprehensive guide to digital entrepreneurship, bridging academic research and industry practice. Morabito provides a strategic overview of the main challenges and trends related to digital entrepreneurship, structured in three parts. Part I focuses on strategy and management issues, guiding readers through the theory and practice of building, implementing and growing new digital ventures and outlining the skills that are necessary for digital entrepreneurs to succeed and lead. Part II focuses on digital business systems, describing the main technological aspects that support and comprise the core infrastructure for digital entrepreneurship, including social media and the Internet of Things. Finally, Part III provides analyses of three core industries in which digital ventures are particularly important: fintech, manufacturing and fashion. Digital Entrepreneurship will appeal to students and researchers in the areas of digital strategy/innovation and information systems management. It will also be of interest to practitioners looking to develop or innovate digital ventures.
Constructing Kanchi
Authors: Stein Emma Natalya
Published: December 2021

This book traces the emergence of the South Indian city of Kanchi as a major royal capital and multireligious pilgrimage destination during the era of the Pallava and Chola dynasties (ca. seventh through thirteenth centuries). It presents the first-ever comprehensive picture of historical Kanchi, locating the city and its more than 100 spectacular Hindu temples at the heart of commercial and artistic exchange that spanned India, Southeast Asia, and China. The author demonstrates that Kanchi was structured with a hidden urban plan, which determined the placement and orientation of temples around a central thoroughfare that was also a burgeoning pilgrimage route. Moving outwards from the city, she shows how the transportation networks, river systems, residential enclaves, and agrarian estates all contributed to the vibrancy of Kanchi's temple life. The construction and ongoing renovation of temples in and around the city, she concludes, has enabled Kanchi to thrive continuously from at least the eighth century, through the colonial period, and up until the present.
An -valued index of symmetry-protected topological phases with on-site finite group symmetry for two-dimensional quantum spin systems
Authors: Ogata Yoshiko
Published: December 2021

We consider symmetry-protected topological phases with on-site finite groupGsymmetry$\beta $for two-dimensional quantum spin systems. We show that they have$H^{3}(G,{\mathbb T})$-valued invariant.
The School Counsellor’s Role in Trauma-Aware Education
Authors: Howard Judith , L’Estrange Lyra , Brown Meegan
Published: December 2021

It is not unusual for school counsellors to be involved in trauma-aware education practice; however, their role is not uniformly defined in the literature nor consistently applied in professional settings. Trauma-aware education is relatively new but rapidly growing in Australia and beyond. It involves supporting students in a neuroscience-informed manner to address the impacts of complex trauma on their capacities to feel safe, to relate, to emotionally regulate, and to learn. Twenty-six school counsellors completed questionnaires, and eight of those joined focus group discussions to explore the role of school counsellors in trauma-aware practice in Queensland, Australia. Drawing on the voices of practitioners, the present report discusses categories of practice that are prioritised by school counsellors and recommends supports to effectively undertake trauma-aware practice in school settings.
Quality and validity of diet quality indices for use in Australian contexts: a systematic review
Authors: Tan Mui Siew , Cheung Ho Ching , McAuley Erynn , Ross Lynda J. , MacLaughlin Helen L.
Published: December 2021

Diet quality indices (DQIs) are tools used to evaluate the overall diet quality against dietary guidelines or known healthy dietary patterns. This review aimed to evaluate DQIs and their validation processes to facilitate decision making in the selection of appropriate DQI for use in Australian contexts. A search of CINAHL, PubMed and Scopus electronic databases was conducted for studies published between January 2010 and May 2020, which validated a DQI, measuring > 1 dimension of diet quality (adequacy, balance, moderation, variety) and was applicable to the Australian context. Data on constructs, scoring, weighting and validation methods (construct validity, criterion validity, reliability and reproducibility) were extracted and summarised. The quality of the validation process was evaluated using COnsensus-based Standards for the selection of health Measurement INstruments Risk of Bias and Joanna Briggs Appraisal checklists. The review identified twenty-seven indices measuring adherence to: national dietary guidelines (n 13), Mediterranean Diet (n 8) and specific population recommendations and chronic disease risk (n 6). Extensiveness of the validation process varied widely across and within categories. Construct validity was the most strongly assessed measurement property, while evaluation of measurement error was frequently inadequate. DQIs should capture multiple dimensions of diet quality, possess a reliable scoring system and demonstrate adequate evidence in their validation framework to support use in the intended context. Researchers need to understand the limitations of newly developed DQIs and interpret results in view of the validation evidence. Future research on DQIs is indicated to improve evaluation of measurement error, reproducibility and reliability.
Application of rigid bronchoscopy for emergent removal of tracheobronchial foreign body in paediatric cases: a learning curve study
Authors: Bin X , Liu L , Fang Q , Tan SH , Tang AZ
Published: December 2021

Objectives

To explore the factors associated with the operative duration for paediatric tracheobronchial foreign body removal by rigid bronchoscopy, and to analyse the learning curve for mastery of the rigid bronchoscopy skill.

Methods

A retrospective study was performed of paediatric cases of tracheobronchial foreign body removal by rigid bronchoscopy in our department from January 2007 to July 2019. Multivariate Cox regression analysis was used to analyse the factors associated with the operative duration. In addition, the learning curves for two doctors were evaluated by curve-fitting regression analysis.

Results

A total of 410 paediatric cases of tracheobronchial foreign body removal by rigid bronchoscopy were evaluated. The operative duration was significantly influenced by the skill of the doctor. The learning curves for both doctor A and doctor B demonstrated two typical phases: an initially rapidly changing learning phase followed by a steady consolidation phase.

Conclusion

The operative duration for paediatric tracheobronchial foreign body removal by rigid bronchoscopy was associated with the skill of the doctor. In order to fully master the rigid bronchoscopy technique, doctors should perform a minimum number of procedures to pass the learning phase and reach the consolidation phase.
Bacteriophages and their microbial hosts in terrestrial biotopes of Antarctica
Authors: Holovan Viktoriia , Andriichuk Olena , Budzanivska Irena , Zelena Pavlina , Kondratiuk Tetiana , Shevchenko Oleksiy
Published: December 2021

Virus diversity in Antarctic biotopes remains understudied. Here, we describe bacteriophages isolated from terrestrial environments, provide data on their natural bacterial hosts and study phage-host systems. Six bacterial isolates (FCKU 539, FCKU 533, FCKU 534, FCKU 538, FCKU 542 and FCKU 540) were recovered and characterized. Isolated bacteria belonged to Pseudomonas genus (Pseudomonas sp., Pseudomonas fluorescens, Pseudomonas putida) with optimal cultivation temperatures of 16–28°C. These bacteria and previously described Bacillus subtilis FCKU 537 were used for analysing virus-host interactions. Six lytic phages were isolated and named P. fluorescens Antarctic virus 1 (PFAV1), P. fluorescens Antarctic virus 2 (PFAV2), P. fluorescens Antarctic virus 3 (PFAV3), P. putida Antarctic virus 4 (PPAV4), Pseudomonas sp. Antarctic virus 5 (PSAV5) and B. subtilis Antarctic virus 6 (BSAV6) in relation to their natural hosts. According to electron microscopy data, these phages belonged to Caudovirales order. Cross-inoculation demonstrated high specificity of all Antarctic phages, which infected only their initial hosts at moderate temperatures. PFAV2 and PFAV3 phages also infected laboratory Pseudomonas savastanoi and P. fluorescens isolates. This paper adds new data on the occurrence and diversity of viruses and their respective bacterial hosts in soil biotopes of Antarctica.
Serotiny in Melocactus matanzanus (Cactaceae) and role of cephalium in dispersal of seeds after the individual's death
Authors: Barrios Duniel , Toledo Sandy , Sánchez Jorge A. , González-Torres Luis R.
Published: December 2021

Serotiny is a strategy in which the retention of mature seeds in parent structures allows plants to cope with environmental variability like heat, drought or fire. Although this phenomenon might be common in Cactaceae, and particularly in Melocactus, it has generally been scarcely addressed. The main goal of our work is to investigate if there are seeds hidden in the cephalium of Melocactus matanzanus and if there are, determine whether or not these seeds maintain their viability. We also discuss some advantages the cephalium may offer as diaspore after the death of individuals. Cephalia collected from dead individuals were divided into four slices and their seeds counted; we also assessed the viability and photoblastic response of the seeds by using growth chambers at 25/30°C, and by a cut test on the seeds that did not germinate. Our results showed retention of viable seeds of different ages in all slices of the cephalium. Seeds were photoblastic positive with germination between 11–22% and viability above 50% in the portion of the lots that did not germinate.
Surface Reconstruction of Microscale Objects Based on Grid-Patterned Structured-Light Measurements
Authors: Wang Yuezong , Chen Jiqiang , Peng Youfan
Published: December 2021

A structured-light projection system was designed for microscale objects with surface heights that ranged from tens to hundreds of microns. The system was composed of a universal projector and microscope system that supported editing the attributes of structured-light patterns in real-time and was capable of projecting microscale patterns. On this basis, reconstructing the metal surfaces of microscale objects based on grid patterns of structured light was investigated, the internal and external parameters of microscope vision and projection systems were calibrated, and an image algorithm for grid-node detection was designed. The results indicated that the proposed method successfully reconstructed the three-dimensional (3D) surface of microscale objects, and the reconstruction results were consistent with the original surfaces. With 95% confidence, the reconstruction precision in the X- and Y-directions was approximately ±4.0 μm and in the Z-direction was approximately ±7.5 μm. The designed system and the proposed method were suitable for 3D-shape measurement of microstructures in microscopic fields and can be adapted to meet a broader range of applications, as compared to current methods.
Can hemp hurd or paper mulch and biochar application improve weed management in matted-row strawberry production systems?
Authors: Gramig Greta G. , Hogstad Samantha K. , Carr Patrick M.
Published: December 2021

During 2015 and 2016, studies were conducted at Absaraka and Dickinson, North Dakota to evaluate the impacts of hemp (applied at 1156 m3 ha−1) and commercial paper mulch, as well as soil-applied biochar (applied at 11.25 m3 ha−1), on weed suppression and strawberry growth during the establishment year, and on weed suppression and strawberry yield during the production year, in a matted row production (MRP) system. During 2015, biochar influenced dry weed biomass only within the hemp mulch, with slightly more weed biomass associated with biochar application compared to zero biochar (3.1 vs 0.4 g m−2), suggesting that biochar may have increased weed germination and/or emergence from beneath hemp mulch. Biochar application also slightly increased soil pH, from 6.9 in non-amended soil to 7.0 in amended soil. Strawberry runner number during 2015 was greater in association with hemp or paper mulch compared to zero mulch (4.5 and 4.9 vs 2.4 runners plant −1, respectively). This result mirrored a similar differential in per berry mass across sites (7.6 and 7.4 vs 6.2 g berry −1 for hemp mulch, paper mulch and zero mulch, respectively). These results may be related to hemp and paper mulch reducing maximum soil temperatures during summer 2015. During the establishment year, both hemp and paper mulch suppressed weeds well compared to zero mulch, although at Absaraka hemp mulch provided slightly better weed suppression than paper mulch. During the production year, both mulches continued to suppress weeds compared to zero mulch at Dickinson. However, at Absaraka, only hemp mulch provided weed suppression compared to zero mulch, possibly because of faster paper degradation caused by greater numbers of large precipitation events and greater relative humidity at Absaraka compared to Dickinson. Weeds were removed from plots during 2015 to allow separation of weed suppression from other possible mulch impacts; therefore, yield data do not reveal striking differences among mulch treatments. Because previous research has demonstrated the impact of weed management during the establishment of strawberries in a matted row system, we concluded that hemp mulch may provide more durable weed suppression compared to paper mulch, which would increase strawberry yield protection in an MRP system. Material cost may be an issue for implementing hemp mulch, as hemp hurd cost was 25 times paper mulch at the application rates used in this study. However, hemp mulch could still be a beneficial option, especially for organic strawberry growers desiring a renewable and environmentally sound replacement for plastic mulch who are able to find affordable local sources of this material.
Is There Semantic Conflict in the Stroop Task?
Authors: Burca Mariana , Beaucousin Virginie , Chausse Pierre , Ferrand Ludovic , Parris Benjamin A. , Augustinova Maria
Published: December 2021

Abstract. This research addressed current controversies concerning the contribution of semantic conflict to the Stroop interference effect and its reduction by a single-letter coloring and cueing procedure. On the first issue, it provides, for the first time, unambiguous evidence for a contribution of semantic conflict to the (overall) Stroop interference effect. The reported data remained inconclusive on the second issue, despite being collected in a considerable sample and analyzed with both classical (frequentist) and Bayesian inferential approaches. Given that in all past Stroop studies, semantic conflict was possibly confounded with either response conflict (e.g., when semantic-associative items [ SKYblue] are used to induce semantic conflict) or with facilitation (when color-congruent items [ BLUEblue] are used as baseline to derive a magnitude for semantic conflict), its genuine contribution to the Stroop interference effect is the most critical result reported in the present study. Indeed, it leaves no doubt – in complete contrast to dominant single-stage response competition models (e.g., Roelofs, 2003 ) – that selection occurs at the semantic level in the Stroop task. The immediate implications for the composite (as opposed to unitary) nature of the Stroop interference effect and other still unresolved issues in the Stroop literature are outlined further.
Laypeople’s Belief of the Influence of Thank-You Gifts on Charitable Giving
Authors: Huang Huiqing , Zhang Yuzhuo , Lv Jieyu , Jiang Tong , Zhang Xi , Chen Xuhai , Luo Yangmei
Published: December 2021

Abstract. Although offering gifts to encourage prosocial behaviors is a popular daily strategy, its underlying mechanism remains unknown. This study investigated the effect of thank-you gifts on charitable giving in laypeople’s beliefs ( N = 1,293). Study 1 showed that laypeople believe thank-you gifts increase charitable giving. Study 2 found that laypeople believe thank-you gifts increase both charitable giving and positive emotions of donors. Study 3 further showed that laypeople’s anticipation of donors’ emotional gain might play a mediating role in the effect of thank-you gifts on charitable giving. Study S1 found that participants’ donated amounts in the benefit-to-others thank-you gifts condition exceeded other conditions on actual donation behavior. These findings emphasize the emotional value of the gift in laypeople’s beliefs.
Measuring Fear of Failure
Authors: Henschel Christine , Iffland Benjamin
Published: December 2021

Abstract. Fear of failure (FF) is a multidimensional construct encompassing anticipated negative consequences deriving from potential failures in evaluative achievement contexts, such as education or sports. The Performance Failure Appraisal Inventory (PFAI; Conroy et al., 2002 ) assesses five threat appraisals associated with FF and has been validated in various cultures, languages, and contexts. To date, there is no instrument measuring FF in an academic context in German. Thus, this study examined the psychometric properties of a German version of the PFAI in a sample of N = 326 university students. Confirmatory factor analyses supported a correlated five-factor structure that has already been established in previous validation studies as well as a bifactor structure. All dimensions demonstrated satisfactory internal consistencies and construct validity. Within the scope of this study, the German version of the PFAI was found to be a psychometrically sound measure to assess FF in an academic context amongst university students.
The Response of Precipitation Extremes to the Twentieth‐ and Twenty‐First‐Century Global Temperature Change in a Comprehensive Suite of CESM1 Large Ensemble Simulation: Revisiting the Role of Forcing Agents Vs. the Role of Forcing Magnitudes
Authors: Xu Yangyang , Lin Lei , Diao Chenrui , Wang Zhili , Bates Susan , Arblaster Julie
Published: December 2021

The response of precipitation extremes (PEs) to global warming is found to be nonlinear in Community Earth System Model version 1 (CESM1) and other global climate models (Pendergrass et al., 2019), which led to the concern that it is not accurate to approximate the response of PE to a single forcing as the difference between simulations with all forcing agents and those that exclude one specific forcing. This calls into question previous model‐based results that the sensitivity of PE with warming due to aerosol forcing is significantly larger than that due to greenhouse gases (GHGs). We reevaluate the PE sensitivity to GHGs and aerosols using available CESM1 ensemble simulations. We show that although the PE response to warming is nonlinear in CESM1, especially for the high warming projected in the twenty‐first‐century, PE sensitivity to aerosols is still significantly stronger than that due to GHGs when the comparison is made within similar warming regimes to avoid the bias induced by the nonlinear behavior. But the difference is smaller than previously estimated. We also conclude that the additivity assumption is largely valid to isolate the PE response due to aerosol forcing from the paired simulations including the “all forcing” experiment when the warming regime is small (e.g., 1°C–2°C in the next few decades when aerosol forcing is projected to decline and becomes a major source of uncertainty for model projection).
Oldest Pole Reversal Shows Early Earth Was Well Suited for Life
Authors: Savitsky Zack
Published: December 2021

Australian rocks 3.25 billion years old preserved the oldest signs of Earth’s stable magnetic field and quickly moving crust, critical elements of life’s evolution.
Duration and Intensity of End‐Permian Marine Anoxia
Authors: Pimentel‐Galvan Michael , Lau Kimberly V. , Maher Katharine , Mukerji Tapan , Lehrmann Daniel J. , Altiner Demir , Payne Jonathan L.
Published: December 2021

Ocean anoxia was an important kill mechanism in the end‐Permian mass extinction and uranium isotope data are among the most powerful tools for quantifying the global extent and duration of ocean deoxygenation due to the dependence of uranium isotope fractionation on bottom‐water redox conditions. Although coherent stratigraphic variation in uranium isotope ratios (δ238U) and uranium concentrations ([U]) indicative of prolonged deoxygenation beginning coincident with the extinction is well established, the precise extent of anoxia and associated uncertainty have yet to be quantified. Uncertainty arises from both noise in the data and imprecise knowledge of key parameters within the uranium cycle. In this study, we use the Monte Carlo method to explore a range of scenarios and their implications for the uranium cycle across the Permian‐Triassic boundary and through the first 1.7 million years of the Triassic. We then compare model predictions against measured data using principal component analysis to identify model runs and associated parameter values most compatible with trends in the observed data. The best‐fitting models indicate a pronounced increase in the extent of seafloor anoxia across the Permian/Triassic transition, reaching 18% of the seafloor (95% CI: [11%, 47%]), lasting anywhere from 20 kyr to 1.2 Myr. There is an inverse relationship between the extent and duration of anoxia in the set of best‐fitting models. This initial pulse of pronounced anoxia is followed by a prolonged aftermath, which continues through the remainder of the study interval, of less extensive, yet still expanded, anoxia covering 7.8% of the seafloor (95% CI: [1.6%, 48.9%]). Both expanded and protracted anoxia are required to fit existing data, with no indication of full re‐oxygenation during the study interval.
Thermal History of Lithosphere Formed Beneath Fast Spreading Ridges: Constraints From the Mantle Transition Zone of the East Pacific Rise at Hess Deep and Oman Drilling Project, Wadi Zeeb, Samail Ophiolite
Authors: Grambling Nadine L. , Dygert Nicholas , Boring Beau , Jean Marlon M. , Kelemen Peter B.
Published: December 2021

We investigate the cooling histories of peridotites and gabbros from localities that expose oceanic lithosphere formed beneath two fast seafloor spreading centers: Hess Deep as recovered from IODP Expedition 345 and ODP Leg 147, and the Oman Ophiolite as sampled by the Oman Drilling Project, ICDP Expedition 5057 (OmanDP). At these locations, relict crust‐mantle transition zones are directly sampled, enabling characterization of the thermal history of the crust‐mantle transition, and by inference, the depth extent of hydrothermal circulation beneath spreading centers. We measured major and trace element abundances in crustal gabbros and mantle peridotites from Hess Deep and OmanDP, and applied major and trace element‐based thermometers. Geospeedometric interpretation of the temperatures suggests similar cooling histories at both locations; cooling rates ranged from 0.02 to 2.6 °C/y from peak temperatures up to 1,350°C. The rates are consistent on either side of the paleo‐Moho (i.e., in the crust and mantle). Models for conductive cooling of the lower oceanic crust predict rates more than two orders of magnitude slower at the crust‐mantle transition zone, while thermal models that invoke deep and efficient hydrothermal circulation throughout the entire crustal section predict rates consistent with our observations. We infer that hydrothermal cooling extended to or near the petrologic Moho beneath the East Pacific Rise and the OmanDP paleo‐spreading center, consistent with the Sheeted Sills model for crustal accretion. Comparison with previously published rates recalculated using the methods we employed suggests the oceanic lower crust is cooled hydrothermally in some places and by conduction at others.
Senescent tumor cells: an overlooked adversary in the battle against cancer
Authors: Park Soon Sang , Choi Yong Won , Kim Jang-Hee , Kim Hong Seok , Park Tae Jun
Published: December 2021

Senescent cells in cancer tissue, including senescent fibroblasts and macrophages, have been reported to increase the malignant potency of cancer cells by secreting senescence-associated secretory phenotype (SASP). Otherwise, Senescence of tumor cells has been believed to inhibit tumor growth by halting the massive proliferation and increasing the chances of immune clearance. In particular, senescent tumor cells (STCs) have been thought that they rarely exist in carcinomas because oncogene-induced senescence needs to be overcome for protumorigenic cells to become malignant. However, recent studies have revealed that a considerable number of STCs are present in cancer tissue, even in metastatic sites. In fact, STCs are widely involved in cancer progression by leading to collective invasion and building a cytokine barrier to protect nonsenescent tumor cells from immune attack. Furthermore, therapy-induced STCs can induce tumor progression and recurrence by increasing stemness. However, obscure causative factors and their heterogeneity in various cancers make it difficult to establish the physiological role of STCs. Here, we summarize and review the current knowledge of the pathophysiology and role of STCs. We also outline the current status of therapeutic strategies for directly removing STCs or modulating the SASPs to maximize the positive functions of STCs while suppressing the negative functions.
A neuroeconomic signature of opioid craving: How fluctuations in craving bias drug-related and nondrug-related value
Authors: Biernacki Kathryn , Lopez-Guzman Silvia , Messinger John C. , Banavar Nidhi V. , Rotrosen John , Glimcher Paul W. , Konova Anna B.
Published: December 2021

How does craving bias decisions to pursue drugs over other valuable, and healthier, alternatives in addiction? To address this question, we measured the in-the-moment economic decisions of people with opioid use disorder as they experienced craving, shortly after receiving their scheduled opioid maintenance medication and ~24 h later. We found that higher cravers had higher drug-related valuation, and that moments of higher craving within-person also led to higher drug-related valuation. When experiencing increased opioid craving, participants were willing to pay more for personalized consumer items and foods more closely related to their drug use, but not for alternative “nondrug-related” but equally desirable options. This selective increase in value with craving was greater when the drug-related options were offered in higher quantities and was separable from the effects of other fluctuating psychological states like negative mood. These findings suggest that craving narrows and focuses economic motivation toward the object of craving by selectively and multiplicatively amplifying perceived value along a “drug relatedness” dimension.
Genomic landscape of a relict fir-associated fungus reveals rapid convergent adaptation towards endophytism
Authors: Yuan Zhilin , Wu Qi , Xu Liangxiong , Druzhinina Irina S , Stukenbrock Eva H , Nieuwenhuis Bart P S , Zhong Zhenhui , Liu Zhong-Jian , Wang Xinyu , Cai Feng , Kubicek Christian P , Shan Xiaoliang , Wang Jieyu , Shi Guohui , Peng Long , Martin Francis M
Published: December 2021

Comparative and pan-genomic analyses of the endophytic fungus Pezicula neosporulosa (Helotiales, Ascomycota) from needles of the relict fir, Abies beshanzuensis, showed expansions of carbohydrate metabolism and secondary metabolite biosynthetic genes characteristic for unrelated plant-beneficial helotialean, such as dark septate endophytes and ericoid mycorrhizal fungi. The current species within the relatively young Pliocene genus Pezicula are predominantly saprotrophic, while P. neosporulosa lacks such features. To understand the genomic background of this putatively convergent evolution, we performed population analyses of 77 P. neosporulosa isolates. This revealed a mosaic structure of a dozen non-recombining and highly genetically polymorphic subpopulations with a unique mating system structure. We found that one idiomorph of a probably duplicated mat1-2 gene was found in putatively heterothallic isolates, while the other co-occurred with mat1-1 locus suggesting homothallic reproduction for these strains. Moreover, 24 and 81 genes implicated in plant cell-wall degradation and secondary metabolite biosynthesis, respectively, showed signatures of the balancing selection. These findings highlight the evolutionary pattern of the two gene families for allowing the fungus a rapid adaptation towards endophytism and facilitating diverse symbiotic interactions.
GPR120 induces regulatory dendritic cells by inhibiting HK2-dependent glycolysis to alleviate fulminant hepatic failure
Authors: Yu Hongshuang , Yang Wanlin , Huang Jiefang , Miao Xiang , Wang Bei , Ren Xiaohui , Gu Yuting , Wang Qiwei , Ding Xinyuan , Guo Xin , Qian Fengtao , Zhang Yanyun , Xu Huanbai , Zheng Leizhen , Jin Min
Published: December 2021

Fulminant hepatic failure (FHF) is a potentially fatal liver disease that is associated with intrahepatic infiltration of inflammatory cells. As the receptor of polyunsaturated long chain fatty acids, GPR120 can regulate cell differentiation, proliferation, metabolism, and immune response. However, whether GPR120 is involved in FHF remains unknown. UsingPropionibacterium acnes(P. acnes)-primed, LPS-induced FHF in mice, we found that interference with GPR120 activity using pharmacological agonist attenuated the severity of the liver injury and mortality of FHF in mice, while a lack of GPR120 exacerbated the disease. GPR120 activation potently alleviated FHF and led to decreased T helper (Th) 1 cell response and expansion of regulatory T cells (Tregs). Interestingly, GPR120 agonist didn’t directly target T cells, but dramatically induced a distinct population of CD11c+MHC IIlowCD80lowCD86lowregulatory DCs in the livers of FHF mice. GPR120 was found to restrict HIF-1α-dependent glycolysis. The augmented HIF-1α stabilization caused by GPR120 antagonism or deletion could be attenuated by the inhibition of ERK or by the activation of AMPK. Through the analysis of the clinical FHF, we further confirmed the activation of GPR120 was negatively associated with the severity in patients. Our findings indicated that GPR120 activation has therapeutic potential in FHF. Strategies to target GPR120 using agonists or free fatty acids (FFAs) may represent a novel approach to FHF treatment.
Trend towards virtual and hybrid conferences may be an effective climate change mitigation strategy
Authors: Tao Yanqiu , Steckel Debbie , Klemeš Jiří Jaromír , You Fengqi
Published: December 2021

Since 2020, the COVID-19 pandemic has urged event holders to shift conferences online. Virtual and hybrid conferences are greener alternatives to in-person conferences, yet their environmental sustainability has not been fully assessed. Considering food, accommodation, preparation, execution, information and communication technology, and transportation, here we report comparative life cycle assessment results of in-person, virtual, and hybrid conferences and consider carbon footprint trade-offs between in-person participation and hybrid conferences. We find that transitioning from in-person to virtual conferencing can substantially reduce the carbon footprint by 94% and energy use by 90%. For the sake of maintaining more than 50% of in-person participation, carefully selected hubs for hybrid conferences have the potential to slash carbon footprint and energy use by two-thirds. Furthermore, switching the dietary type of future conferences to plant-based diets and improving energy efficiencies of the information and communication technology sector can further reduce the carbon footprint of virtual conferences.
Charge-transfer regulated visible light driven photocatalytic H2 production and CO2 reduction in tetrathiafulvalene based coordination polymer gel
Authors: Verma Parul , Singh Ashish , Rahimi Faruk Ahamed , Sarkar Pallavi , Nath Sukhendu , Pati Swapan Kumar , Maji Tapas Kumar
Published: December 2021

The much-needed renewable alternatives to fossil fuel can be achieved efficiently and sustainably by converting solar energy to fuels via hydrogen generation from water or CO2 reduction. Herein, a soft processable metal-organic hybrid material is developed and studied for photocatalytic activity towards H2 production and CO2 reduction to CO and CH4 under visible light as well as direct sunlight irradiation. A tetrapodal low molecular weight gelator (LMWG) is synthesized by integrating tetrathiafulvalene (TTF) and terpyridine (TPY) derivatives through amide linkages and results in TPY-TTF LMWG. The TPY-TTF LMWG acts as a linker, and self-assembly of this gelator molecules with ZnII ions results in a coordination polymer gel (CPG); Zn-TPY-TTF. The Zn-TPY-TTF CPG shows high photocatalytic activity towards H2 production (530 μmol g−1h−1) and CO2 reduction to CO (438 μmol g−1h−1, selectivity > 99%) regulated by charge-transfer interactions. Furthermore, in situ stabilization of Pt nanoparticles on CPG (Pt@Zn-TPY-TTF) enhances H2 evolution (14727 μmol g−1h−1). Importantly, Pt@Zn-TPY-TTF CPG produces CH4 (292 μmol g−1h−1, selectivity > 97%) as CO2 reduction product instead of CO. The real-time CO2 reduction reaction is monitored by in situ DRIFT study, and the plausible mechanism is derived computationally.
R-loop proximity proteomics identifies a role of DDX41 in transcription-associated genomic instability
Authors: Mosler Thorsten , Conte Francesca , Longo Gabriel M. C. , Mikicic Ivan , Kreim Nastasja , Möckel Martin M. , Petrosino Giuseppe , Flach Johanna , Barau Joan , Luke Brian , Roukos Vassilis , Beli Petra
Published: December 2021

Transcription poses a threat to genomic stability through the formation of R-loops that can obstruct progression of replication forks. R-loops are three-stranded nucleic acid structures formed by an RNA–DNA hybrid with a displaced non-template DNA strand. We developed RNA–DNA Proximity Proteomics to map the R-loop proximal proteome of human cells using quantitative mass spectrometry. We implicate different cellular proteins in R-loop regulation and identify a role of the tumor suppressor DDX41 in opposing R-loop and double strand DNA break accumulation in promoters. DDX41 is enriched in promoter regions in vivo, and can unwind RNA–DNA hybrids in vitro. R-loop accumulation upon loss of DDX41 is accompanied with replication stress, an increase in the formation of double strand DNA breaks and transcriptome changes associated with the inflammatory response. Germline loss-of-function mutations in DDX41 lead to predisposition to acute myeloid leukemia in adulthood. We propose that R-loop accumulation and genomic instability-associated inflammatory response may contribute to the development of familial AML with mutated DDX41.
Reinforcing the supply chain of umifenovir and other antiviral drugs with retrosynthetic software
Authors: Lin Yingfu , Zhang Zirong , Mahjour Babak , Wang Di , Zhang Rui , Shim Eunjae , McGrath Andrew , Shen Yuning , Brugger Nadia , Turnbull Rachel , Trice Sarah , Jasty Shashi , Cernak Tim
Published: December 2021

The global disruption caused by the 2020 coronavirus pandemic stressed the supply chain of many products, including pharmaceuticals. Multiple drug repurposing studies for COVID-19 are now underway. If a winning therapeutic emerges, it is unlikely that the existing inventory of the medicine, or even the chemical raw materials needed to synthesize it, will be available in the quantities required. Here, we utilize retrosynthetic software to arrive at alternate chemical supply chains for the antiviral drug umifenovir, as well as eleven other antiviral and anti-inflammatory drugs. We have experimentally validated four routes to umifenovir and one route to bromhexine. In one route to umifenovir the software invokes conversion of six C–H bonds into C–C bonds or functional groups. The strategy we apply of excluding known starting materials from search results can be used to identify distinct starting materials, for instance to relieve stress on existing supply chains.
Structure-guided bifunctional molecules hit a DEUBAD-lacking hRpn13 species upregulated in multiple myeloma
Authors: Lu Xiuxiu , Sabbasani Venkata R. , Osei-Amponsa Vasty , Evans Christine N. , King Julianna C. , Tarasov Sergey G. , Dyba Marzena , Das Sudipto , Chan King C. , Schwieters Charles D. , Choudhari Sulbha , Fromont Caroline , Zhao Yongmei , Tran Bao , Chen Xiang , Matsuo Hiroshi , Andresson Thorkell , Chari Raj , Swenson Rolf E. , Tarasova Nadya I. , Walters Kylie J.
Published: December 2021

Proteasome substrate receptor hRpn13 is a promising anti-cancer target. By integrated in silico and biophysical screening, we identified a chemical scaffold that binds hRpn13 with non-covalent interactions that mimic the proteasome and a weak electrophile for Michael addition. hRpn13 Pru domain binds proteasomes and ubiquitin whereas its DEUBAD domain binds deubiquitinating enzyme UCHL5. NMR revealed lead compound XL5 to interdigitate into a hydrophobic pocket created by lateral movement of a Pru β-hairpin with an exposed end for Proteolysis Targeting Chimeras (PROTACs). Implementing XL5-PROTACs as chemical probes identified a DEUBAD-lacking hRpn13 species (hRpn13Pru) present naturally with cell type-dependent abundance. XL5-PROTACs preferentially target hRpn13Pru, causing its ubiquitination. Gene-editing and rescue experiments established hRpn13 requirement for XL5-PROTAC-triggered apoptosis. These data establish hRpn13 as an anti-cancer target for multiple myeloma and introduce an hRpn13-targeting scaffold that can be optimized for preclinical trials against hRpn13Pru-producing cancer types.
Ontogenetic shifts from social to experiential learning drive avian migration timing
Authors: Abrahms Briana , Teitelbaum Claire S. , Mueller Thomas , Converse Sarah J.
Published: December 2021

Migrating animals may benefit from social or experiential learning, yet whether and how these learning processes interact or change over time to produce observed migration patterns remains unexplored. Using 16 years of satellite-tracking data from 105 reintroduced whooping cranes, we reveal an interplay between social and experiential learning in migration timing. Both processes dramatically improved individuals’ abilities to dynamically adjust their timing to track environmental conditions along the migration path. However, results revealed an ontogenetic shift in the dominant learning process, whereby subadult birds relied on social information, while mature birds primarily relied on experiential information. These results indicate that the adjustment of migration phenology in response to the environment is a learned skill that depends on both social context and individual age. Assessing how animals successfully learn to time migrations as environmental conditions change is critical for understanding intraspecific differences in migration patterns and for anticipating responses to global change.
Superspreading quantified from bursty epidemic trajectories
Authors: Kirkegaard Julius B. , Sneppen Kim
Published: December 2021

The quantification of spreading heterogeneity in the COVID-19 epidemic is crucial as it affects the choice of efficient mitigating strategies irrespective of whether its origin is biological or social. We present a method to deduce temporal and individual variations in the basic reproduction number directly from epidemic trajectories at a community level. Using epidemic data from the 98 districts in Denmark we estimate an overdispersion factor k for COVID-19 to be about 0.11 (95% confidence interval 0.08–0.18), implying that 10 % of the infected cause between 70 % and 87 % of all infections.
Efficacy of a multidisciplinary care protocol for the treatment of operated hip fracture patients
Authors: Salvador-Marín Jorge , Ferrández-Martínez Francisco Javier , Lawton Cort D. , Orozco-Beltrán Domingo , Martínez-López Jose Fernando , Kelly Bryan T. , Marzo-Campos Juan Carlos
Published: December 2021

To assess the effects of a multidisciplinary care protocol on cost, length of hospital stay (LOS), and mortality in hip-fracture-operated patients over 65 years. Prospective cohort study between 2011 and 2017. The unexposed group comprised patients who did not receive care according to the multidisciplinary protocol, while the exposed group did. Variables analyzed were demographics, medical comorbidities, treatment, blood parameters, surgical delay, LOS, re-admissions, mortality, and a composite outcome considering in-hospital mortality and/or LOS > 10 days. We performed a Poisson regression and cost analysis. The cohort included 681 patients: 310 unexposed and 371, exposed. The exposed group showed a shorter surgical delay (3.0 vs. 3.6 days; p < 0.001), and a higher proportion received surgery within 48 h (46.1% vs. 34.2%, p = 0.002). They also showed lower rates of 30-day readmission (9.4% vs. 15.8%, p = 0.012), 30-day mortality (4.9% vs. 9.4%, p = 0.021), in-hospital mortality (3.5% vs. 7.7%; p = 0.015), and LOS (8.4 vs. 9.1 days, p < 0.001). Multivariable analysis showed a protective effect of the protocol on the composite outcome (risk ratio 0.62, 95% CI 0.48–0.80, p < 0.001). Hospital costs were reduced by EUR 112,153.3. A multidisciplinary shared care protocol was associated with a reduction in the LOS, surgical delay, 30-day readmissions, and in-hospital and 30-day mortality, in hip-fracture-operated patients.
A retrospective analysis of fibrinolytic and adjunctive antithrombotic treatment during cardiopulmonary resuscitation
Authors: Weiss Armin , Frisch Christoph , Hornung Rouven , Baubin Michael , Lederer Wolfgang
Published: December 2021

Synergistic effects of fibrinolytic and additional antithrombotic treatment during cardiopulmonary resuscitation in out-of-hospital cardiac arrest of assumed cardiac origin were evaluated retrospectively. Data were drawn from electronic files of the physician-staffed Emergency Medical Services Tyrol. During a 22-month observation period 53 adult patients were treated with tenecteplase (mean 7641 IU), 19 (32.1%) of whom received additional antithrombotic treatment with heparin (4000–5000 IU) and acetylsalicylic acid (250–500 mg). Lasting return of spontaneous circulation occurred in four of 34 patients who received fibrinolytic treatment only and in seven of 19 patients with additional antithrombotic treatment (p = 0.037). Four of five patients who were discharged from hospital had received additional antithrombotic treatment during CPR and were in appropriate neurological status (CPC 1). Considering the small sample size in this retrospective study, the argument may be still be made that fibrinolytic and adjunctive antithrombotic treatment during cardiopulmonary resuscitation in out-of-hospital cardiac arrest of assumed cardiac origin may increase the chances for survival.
Moxifloxacin releasing intraocular implant based on a cross-linked hyaluronic acid membrane
Authors: Kim Dong Ju , Jung Mi-Young , Park Joo-Hee , Pak Ha-Jin , Kim Martha , Chuck Roy S. , Park Choul Yong
Published: December 2021

Intraocular antibiotic delivery is an important technique to prevent bacterial infection after ophthalmic surgery, such as cataract surgery. Conventional drug delivery methods, such as antibiotic eye drops, have limitations for intraocular drug delivery due to the intrinsic barrier effect of the cornea. Therefore, frequent instillation of antibiotic eyedrops is necessary to reach a sufficient bactericidal concentration inside the eye. In this study, an intraocular implant, MXF-HA, that combines hyaluronic acid (HA) and moxifloxacin (MXF) was developed to increase the efficiency of intraocular drug delivery after surgery. MXF-HA is manufactured as a thin, transparent, yellow-tinted membrane. When inserted into the eye in a dry state, MXF-HA is naturally hydrated and settles in the eye, and the MXF contained therein is delivered by hydrolysis of the polymer over time. It was confirmed through in vivo experiments that MXF delivery was maintained in the anterior chamber of the eye at a concentration sufficient to inhibit Pseudomonas aeruginosa and Staphylococcus aureus for more than 5 days after implantation. These results suggest that MXF-HA can be utilized as a potential drug delivery method for the prevention and treatment of bacterial infections after ophthalmic surgery.
Altered cardiac mitochondrial dynamics and biogenesis in rat after short-term cocaine administration
Authors: Wen Shuheng , Unuma Kana , Funakoshi Takeshi , Aki Toshihiko , Uemura Koichi
Published: December 2021

Abuse of the potent psychostimulant cocaine is widely established to have cardiovascular consequences. The cardiotoxicity of cocaine is mainly associated with oxidative stress and mitochondrial dysfunction. Mitochondrial dynamics and biogenesis, as well as the mitochondrial unfolded protein response (UPRmt), guarantee cardiac mitochondrial homeostasis. Collectively, these mechanisms act to protect against stress, injury, and the detrimental effects of chemicals on mitochondria. In this study, we examined the effects of cocaine on cardiac mitochondrial dynamics, biogenesis, and UPRmt in vivo. Rats administered cocaine via the tail vein at a dose of 20 mg/kg/day for 7 days showed no structural changes in the myocardium, but electron microscopy revealed a significant increase in the number of cardiac mitochondria. Correspondingly, the expressions of the mitochondrial fission gene and mitochondrial biogenesis were increased after cocaine administration. Significant increase in the expression and nuclear translocation of activating transcription factor 5, the major active regulator of UPRmt, were observed after cocaine administration. Accordingly, our findings show that before any structural changes are observable in the myocardium, cocaine alters mitochondrial dynamics, elevates mitochondrial biogenesis, and induces the activation of UPRmt. These alterations might reflect cardiac mitochondrial compensation to protect against the cardiotoxicity of cocaine.