bluuebunny/crossref_metadata_2025 huggingface dataset:
The Importance of Epigenetics in Diagnostics and Treatment of Major Depressive Disorder
Authors: Czarny Piotr , Białek Katarzyna , Ziółkowska Sylwia , Strycharz Justyna , Barszczewska Gabriela , Sliwinski Tomasz
Published: March 2021

Recent studies imply that there is a tight association between epigenetics and a molecular mechanism of major depressive disorder (MDD). Epigenetic modifications, i.e., DNA methylation, post-translational histone modification and interference of microRNA (miRNA) or long non-coding RNA (lncRNA), are able to influence the severity of the disease and the outcome of the therapy. This article summarizes the most recent literature data on this topic, i.e., usage of histone deacetylases as therapeutic agents with an antidepressant effect and miRNAs or lncRNAs as markers of depression. Due to the noteworthy potential of the role of epigenetics in MDD diagnostics and therapy, we have gathered the most relevant data in this area.
The Impact of Israeli and Saudi Arabian Geopolitical Risks on the Lebanese Financial Market
Authors: Mansour-Ichrakieh Layal
Published: March 2021

Hezbollah is best defined in geopolitics as the Iranian ideology proxy that aims to grow the Persian footprint and to gain geopolitical strategic depth in the region. Its role continuously requires geopolitical conflicts, mainly against Saudi Arabia and Israel—for resistance, ideological, and geopolitical purposes. Being a state within a state, Hezbollah militia makes sovereign geopolitical decisions and forces Lebanon to pay for the consequences. This is the first economic study that empirically investigates under vector autoregression (VAR) models the dynamic causal relationship between Saudi Arabia and Israel’s geopolitical risks and Lebanon’s financial stability and economic activity. The results show that Saudi Arabia and Israel’s geopolitical risks affect Lebanon’s economy differently. Economic and financial stability cannot be promoted independently of regional geopolitical conflict.
Choosing Factors for the Vietnamese Stock Market
Authors: Ryan Nina , Ruan Xinfeng , Zhang Jin E. , Zhang Jing A.
Published: March 2021

In this paper, we test the applicability of different Fama–French (FF) factor models in Vietnam, we investigate the value factor redundancy and examine the choice of the profitability factor. Our empirical evidence shows that the FF five-factor model has more explanatory power than the FF three-factor model. The value factor remains important after the inclusion of profitability and investment factors. Operating profitability performs better than cash and return-on-equity (ROE) profitability as a proxy for the profitability factor in FF factor modeling. The value factor and operating profitability have the biggest marginal contribution to a maximum squared Sharpe ratio for the five-factor model factors, highlighting the value factor (HML) non-redundancy in describing stock returns in Vietnam.
Evolutionary Overview and Prediction of Themes in the Field of Land Degradation
Authors: Lu Xinhai , Zhang Yanwei , Lin Chaoran , Wu Feng
Published: March 2021

Land degradation has become one of the major global environmental problems threatening human well-being. Whether degraded land can be restored has a profound effect on the achievement of the 2030 UN Sustainable Development Goals. Therefore, the ways by which to identify the current research status and potential research topics in the massive scientific literature data in the field of land degradation is a crucial issue for scientific research institutions in various countries. In view of the shortcomings in the current research on the thematic evolution and thematic and thematic prediction, such as the ignorance of random features during scientific innovation, the defects of manual classification, and the difficulty of identifying technical terms, this research proposes a new combined method. First, the Latent Dirichlet Allocation (LDA) algorithm in machine learning is used to capture the potential clustering of themes in the literature sample set of land degradation research. The distribution characteristics and evolution of themes in each period are then analyzed. The method is combined with the Hidden Markov Model (HMM), which contains double stochastic process to quantitatively predict the trend of future thematic evolution. Finally, the above-mentioned combined method is used to analyze the evolution characteristics and future development trends of the themes in the field of land degradation. Comparative experiments show that the method in this study is effective and practical. The research results show that rangeland degradation, surface temperature, island, soil degradation, water quality, crop productivity and restoration are important research topics in the field of land degradation in the future. In addition, based on the advantages of this model, this model can be widely used in the thematic evolution and prediction analysis of different research fields in land use science.
Quantitatively Determining the Priorities of Regional Ecological Compensation for Cultivated Land in Different Main Functional Areas: A Case Study of Hubei Province, China
Authors: Chen Xiaojie , Wang Jing
Published: March 2021

With the rapid economic growth and urbanisation process, a large amount of cultivated land has been permanently transformed into urban land. The protection of cultivated land has received widespread attention, and ecological compensation has been an effective means of restraining the decrease in cultivated land. Different from previous approaches to and methods of studying cultivated land’s ecological compensation, this study proposes a new behavioural perspective. Reasonably determining the priority of ecological compensation and the amount of compensation constitutes the core of building a regional ecological compensation mechanism. On the basis of the main functional area, this paper defines the area of ecological compensation and payment for cultivated land and determines the corresponding order and level by measuring the ecosystem’s service value in Hubei Province. The results show that the order and quantity of ecological compensation for cultivated land are spatially heterogeneous, and that the order and quantity of compensation (payment) in different main functional areas have a certain correlation. That is, when the order of compensation (payment) is higher, the quantity is larger. The results have a certain reference value for the reform of cultivated land’s ecological compensation and the innovation of financial payment mechanisms, particularly in the context of developing countries.
Formation, Structure and Magnetic Properties of MFe2O4@SiO2 (M = Co, Mn, Zn, Ni, Cu) Nanocomposites
Authors: Dippong Thomas , Levei Erika Andrea , Cadar Oana
Published: March 2021

The formation, structure, and thermal and magnetic properties of MFe2O4@SiO2 (M = Co, Mn, Zn, Ni, Cu) (60% MFe2O4/40% SiO2) nanocomposites produced by a modified sol-gel method, followed by annealing at 300, 600, 900 and 1200 °C, were studied. The thermal analysis and Fourier transform infrared spectroscopy showed the formation of metal-glyoxylates below 210 °C and their decomposition into the corresponding ferrite around 300 °C. The evolution of crystalline phases and variation of crystallite sizes differs from ferrite to ferrite and depends on the annealing temperature. The magnetic measurements revealed the dependence of saturation and remanent magnetization, coercivity, and anisotropy on ferrite type, annealing temperature, and particle size. By annealing the nanocomposites (NCs) at 1200 °C paramagnetic MnFe2O4, CoFe2O4, NiFe2O4 and CuFe2O4 and antiferromagnetic ZnFe2O4 are obtained.
Evaluation of the Effects of Cement and Lime with Rice Husk Ash as an Additive on Strength Behavior of Coastal Soil
Authors: Eliaslankaran Zahraalsadat , Daud Nik Norsyahariati Nik , Yusoff Zainuddin Md. , Rostami Vahid
Published: March 2021

Coastal accretion and erosion are unavoidable processes as some coastal sediments undergo modification and stabilization. This study was conducted to investigate the geotechnical behavior of soil collected from Bagan Lalang coast and treated with lime, cement, and rice husk ash (RHA) to design a low-cost alternative mixture with environmentally friendly characteristics. Laboratory tests were carried out to analyze the physical properties of the soil (Atterberg limits and compaction properties), together with mechanical characteristics (direct shear and unconfined compressive strength (UCS) tests) to determine the effect of different ratios of stabilizer/pozzolan on the coastal soil and the optimum conditions for each mixture. Part of the purpose of this study was also to analyze the shear behavior of the coastal soil and monitor the maximum axial compressive stress that the treated specimens can bear under zero confining pressure. Compared to the natural soil, the soil treated with lime and rice husk ash (LRHA) in the ratio of 1:2 (8% lime content) showed a tremendous increase in shear stress under the normal stress of 200 kPa. The strength parameters such as the cohesion (c) and internal friction angle (ϕ) values showed a significant increase. Cohesion values increased considerably in samples cured for 90 days compared to specimens cured for 7 days with additional LRHA in the ratio of 1:2 (28%).
Evaluation of Micro-Mechanism and High- and Low-Temperature Rheological Properties of Disintegrated High Volume Crumb Rubber Asphalt (DHVRA)
Authors: Li Wei , Han Sen , Fu Xiaokang , Huang Ke
Published: March 2021

The aims of this paper are to prepare disintegrated high volume crumb rubber asphalt (DHVRA) with low viscosity, good workability and low-temperature performance by adding disintegrating agent (DA) in the preparation process, and to further analyze the disintegrating mechanism and evaluated high-temperature and low-temperature rheological properties. To obtain DHVRA with excellent comprehensive performance, the optimum DA dosage was determined. Based on long-term disintegrating tests and the Fluorescence Microscopy (FM) method, the correlations between key indexes and crumb rubber (CR) particle diameter was analyzed, and the evaluation indicator and disintegrating stage division standard were put forward. Furthermore, Fourier transform infrared spectroscopy (FT-IR) and Gel Permeation Chromatography (GPC) was used to reveal the reaction mechanism, and the contact angle test method was adopted to evaluate the surface free energy (SFE). In addition, the high-temperature and low-temperature rheological properties were measured, and the optimum CR content was proposed. Results indicated that the optimum DA dosage was 7.5‰, and the addition of DA promoted the melt decomposition of CR, reduced the viscosity and improved the storage stability. The 135 °C rotational viscosity (RV) of DHVRA from mixing for 3 h could be reduced to 1.475 Pa·s, and the softening point difference was even less than 2 °C. The linear correlation between 135 °C RV and the diameter of CR particle in rubber asphalt system was as high as 0.968, and the viscosity decay rate (VDR) was used as the standard to divide the disintegrating process into a fast disintegrating stage, stable disintegrating stage and slight disintegrating stage. Compared to common rubber asphalt (CRA), DHVRA has an absorption peak at 960 cm−1 caused by trans olefin = C-H, and higher molecular weight and polar component of surface energy. Compared with CRA, although the high-temperature performance of DHVRA decreases slightly, the low-temperature relaxation ability can be greatly improved.
Influence of Tool Geometry and Process Parameters on the Properties of Friction Stir Spot Welded Multiple (AA 5754 H111) Aluminium Sheets
Authors: Labus Zlatanovic Danka , Balos Sebastian , Bergmann Jean , Rasche Stefan , Pecanac Milan , Goel Saurav
Published: March 2021

Friction stir spot welding is an emerging spot-welding technology that offers opportunities for joining a wide range of materials with minimum energy consumption. To increase productivity, the present work addresses production challenges and aims to find solutions for the lap-welding of multiple ultrathin sheets with maximum productivity. Two convex tools with different edge radii were used to weld four ultrathin sheets of AA5754-H111 alloy each with 0.3 mm thickness. To understand the influence of tool geometries and process parameters, coefficient of friction (CoF), microstructure and mechanical properties obtained with the Vickers microhardness test and the small punch test were analysed. A scanning acoustic microscope was used to assess weld quality. It was found that the increase of tool radius from 15 to 22.5 mm reduced the dwell time by a factor of three. Samples welded with a specific tool were seen to have no delamination and improved mechanical properties due to longer stirring time. The rotational speed was found to be the most influential parameter in governing the weld shape, CoF, microstructure, microhardness and weld efficiency. Low rotational speeds caused a 14.4% and 12.8% improvement in joint efficiency compared to high rotational speeds for both tools used in this investigation.
Frequent Methylation of RASSF1 and RARB in Urine Sediments From Patients with Early Stage Prostate Cancer
Authors: Daniūnaitė Kristina , Berezniakovas Artūras , Jankevičius Feliksas , Laurinavičius Arvydas , Lazutka Juozas , Jarmalaitė Sonata
Published: March 2021

Background. Prostate cancer (PCa) is the second most prevalent malignancy among males, characterized by high mortality rates. Aberrant DNA methylation in promoters of tumor suppressor genes is an early and frequent event during prostate carcinogenesis. Modern techniques allow a sensitive detection of DNA methylation biomarkers in bodily fluids from cancer patients offering a noninvasive tool for PCa monitoring. Our study aimed at the analysis of DNA methylation in urine sediments from PCa patients for the selection of most informative noninvasive biomarkers. Material and Methods. Real-time methylation-specific polymerase chain reaction was used for the detection of methylated RASSF1, RARB, and GSTP1 genes in catheterized urine specimens from 34 patients with biopsy-proven early or medium stage PCa. Results. At least one gene was methylated in urine sediments from 28 cases with PCa, with a sensitivity of the test reaching 82%. RASSF1 was methylated in 71% (24 of 34), RARB in 44% (15 of 34), and GSTP1 in 3% (1 of 34) of the specimens. High level of methylation (≥50%) in RARB and RASSF1 genes was detected in 40% and 20% of cases, respectively. A significant association was observed between high level of RARB methylation and Gleason score (P=0.01), while methylation of at least one gene occurred more frequently in urine DNA of older patients (P=0.02). Conclusions. Results of our study show a high sensitivity of DNA methylation biomarkers, especially RASSF1 and RARB, for the early and noninvasive detection of PCa.
Diagnostic Value of Frequently Implemented Provocative Tests in the Assessment of Shoulder Pain—A Glimpse of Current Practice
Authors: Rosenberg Nahum
Published: March 2021

Initial evaluation of chronic shoulder disability is a diagnostic challenge due to the anatomic complexity of the shoulder joints. For this purpose, several diagnostic tools utilizing provocative testing exist, but only a few have a reliable basis for their diagnostic value. Therefore, objectively determining the predictive value of these tests in identifying the precise anatomical source for disability—subacromial, intraarticular or other—is essential in order to proceed with further imaging evaluations for final objective diagnoses. Using validated clusters of provocative tests should improve their diagnostic values.
Influence of the Submerged Entry Nozzle’s Bottom Well on the Characteristics of Its Exit Jets
Authors: Gonzalez-Trejo Jesus , Real-Ramirez Cesar A. , Miranda-Tello Jose Raul , Gabbasov Ruslan , Carvajal-Mariscal Ignacio , Sanchez-Silva Florencio , Cervantes-de-la-Torre Francisco
Published: March 2021

In vertical continuous casting machines the liquid steel from the tundish is poured into the mold through the Submerged Entry Nozzle (SEN). The shape and direction of the SEN exit jets affect the liquid steel dynamics inside the mold. This work quantifies the effect of the SEN pool on the principal characteristics of the jets emerging from it, precisely, the shape, the spread angles, and the mold impact point. Experimental and numerical simulations were carried out using a SEN simplified model, a square-shaped bore nozzle with square-shaped outlet ports whose length is minimal. These experiments showed two well-defined behaviors. When a single vortex dominates the hydrodynamics inside the simplified SEN, the exit jets spread out and are misaligned about the mold’s central plane. On the contrary, when the inner flow pattern shows two vortexes, the exit jets are compact and parallel to the mold wide walls. The measured difference on the jet’s falling angles is 5°, approximately, which implies that in an actual casting machine, the impingement point at the narrow mold wall would have a variation of 0.150 m. This hydrodynamic analysis would help design new SENs for continuous casting machines that improve steel quality.
Hydraulic and Thermal Performance of Microchannel Heat Sink Inserted with Pin Fins
Authors: Xie Guo-Fu , Zhao Lei , Dong Yuan-Yuan , Li Yu-Guang , Zhang Shang-Lin , Yang Chen
Published: March 2021

With the development of micromachining technologies, a wider use of microchannel heat sink (MCHS) is achieved in many fields, especially for cooling electronic chips. A microchannel with a width of 500 μm and a height of 500 μm is investigated through the numerical simulation method. Pin fins are arranged at an inclined angle of 0°, 30°, 45°, and 60°, when arrangement method includes in-lined pattern and staggered pattern. The effects of inclined angle and arrangement method on flow field and temperature field of MCHSs are studied when Reynolds number ranges from 10 to 300. In addition to this, quantitative analyses of hydraulic and thermal performance are also discussed in this work. With the increase of inclined angle, the variation of friction factor and Nusselt number do not follow certain rules. The best thermal performance is achieved in MCHS with in-lined fines at an inclined angle of 30° accompanied with the largest friction factor. Arrangement method of pin fins plays a less significant role compared with inclined angle from a general view, particularly in the Reynolds number range of 100~300.
Loading and Release of Charged and Neutral Fluorescent Dyes into and from Mesoporous Materials: A Key Role for Sensing Applications
Authors: Climent Estela , Hecht Mandy , Rurack Knut
Published: March 2021

The aim of this study is to determine the efficiency of loading and release of several zwitterionic, neutral, anionic and cationic dyes into/from mesoporous nanoparticles to find the optimum loading and release conditions for their application in detection protocols. The loading is carried out for MCM-41 type silica supports suspended in phosphate-buffered saline (PBS) buffer (pH 7.4) or in acetonitrile, involving the dyes (rhodamine B chloride, rhodamine 101 chloride, rhodamine 101 perchlorate, rhodamine 101 inner salt, meso-(4-hydroxyphenyl)-boron–dipyrromethene (BODIPY), sulforhodamine B sodium salt and fluorescein 27). As a general trend, rhodamine-based dyes are loaded with higher efficiency, when compared with BODIPY and fluorescein dyes. Between the rhodamine-based dyes, their charge and the solvent in which the loading process is carried out play important roles for the amount of cargo that can be loaded into the materials. The delivery experiments carried out in PBS buffer at pH 7.4 reveal for all the materials that anionic dyes are more efficiently released compared to their neutral or cationic counterparts. The overall best performance is achieved with the negatively charged sulforhodamine B dye in acetonitrile. This material also shows a high delivery degree in PBS buffer.
High Gain Triple-Band Metamaterial-Based Antipodal Vivaldi MIMO Antenna for 5G Communications
Authors: Saeidi Tale , Ismail Idris , Noghanian Sima , Alhawari Adam R. H. , Abbasi Qammer H. , Imran Muhammad Ali , Zeain M. Y. , Ali Shahid M.
Published: March 2021

This paper presents a miniaturized dual-polarized Multiple Input Multiple Output (MIMO) antenna with high isolation. The antenna meets the constraints of sub-6 GHz 5G and the smartphones’ X-band communications. A vertically polarized modified antipodal Vivaldi antenna and a horizontally polarized spiral antenna are designed and integrated, and then their performance is investigated. Three frequency bands of 3.8 GHz, 5.2 GHz, and 8.0 GHz are considered, and the proposed dual-polarized antenna is studied. High isolation of greater than 20 dB is obtained after integration of metamaterial elements, and without applying any other decoupling methods. The proposed triple-band metamaterial-based antenna has 1.6 GHz bandwidth (BW) (2.9 GHz–4.5 GHz), 13.5 dBi gain, and 98% radiation efficiency at 3.8 GHz. At 5.2 GHz it provides 1.2 GHz BW, 9.5 dBi gain, and 96% radiation efficiency. At 8.0 GHz it has 1 GHz BW, 6.75 dBi gain, and 92% radiation efficiency. Four antenna elements (with eight ports) were laid out orthogonally at the four corners of a mobile printed circuit board (PCB) to be utilized as a MIMO antenna for 5G communications. The performance of the MIMO antenna is examined and reported.
Ectopic Spacer Acquisition in Streptococcus thermophilus CRISPR3 Array
Authors: Achigar Rodrigo , Scarrone Martina , Rousseau Geneviève M. , Philippe Cécile , Machado Felipe , Duvós Valentina , Campot María Pía , Dion Moïra B. , Shao Yuyu , Pianzzola María Julia , Moineau Sylvain
Published: March 2021

Streptococcus thermophilus relies heavily on two type II-A CRISPR-Cas systems, CRISPR1 and CRISPR3, to resist siphophage infections. One hallmark of these systems is the integration of a new spacer at the 5′ end of the CRISPR arrays following phage infection. However, we have previously shown that ectopic acquisition of spacers can occur within the CRISPR1 array. Here, we present evidence of the acquisition of new spacers within the array of CRISPR3 of S. thermophilus. The analysis of randomly selected bacteriophage-insensitive mutants of the strain Uy01 obtained after phage infection, as well as the comparison with other S. thermophilus strains with similar CRISPR3 content, showed that a specific spacer within the array could be responsible for misguiding the adaptation complex. These results also indicate that while the vast majority of new spacers are added at the 5′ end of the CRISPR array, ectopic spacer acquisition is a common feature of both CRISPR1 and CRISPR3 systems in S. thermophilus, and it can still provide phage resistance. Ectopic spacer acquisition also appears to have occurred naturally in some strains of Streptococcus pyogenes, suggesting that it is a general phenomenon, at least in type II-A systems.
Top-Down Synthesis of NaP Zeolite from Natural Zeolite for the Higher Removal Efficiency of Cs, Sr, and Ni
Authors: Hong Seokju , Um Wooyong
Published: March 2021

A solid phase of natural zeolite was transformed to Na-zeolite P (NaP zeolite) by a “top-down approach” hydrothermal reaction using 3 M of NaOH solution in a 96 °C oven. Time-dependent X-ray diffraction (XRD), Fourier-transform infrared (FT-IR), XRF, and scanning electron microscopy (SEM) analysis as well as kinetic, isotherm, and cation exchange capacity experiments were performed to understand the mechanism of mineral transition from natural zeolite to NaP zeolite. The XRD crystal peaks of the natural zeolite decreased (decrystallization phase) first, and then the NaP zeolite XRD crystal peaks increased gradually (recrystallization phase). From the XRF results, the dissolution rate of Si was slow in the recrystallization phase, while it was rapid in the decrystallization phase. The specific surface area measured by BET analysis was higher in NaP zeolite (95.95 m2/g) compared to that of natural zeolite (31.35 m2/g). Furthermore, pore structure analysis confirmed that NaP zeolites have more micropores than natural zeolite. In the kinetic experiment, the results showed that the natural zeolite and NaP zeolite were well matched with a pseudo-second-order kinetic model, and reached equilibrium within 24 h. The isotherm experiment results confirmed that both zeolites were well matched with the Langmuir isotherm, and the maximum removal capacity (Qmax) values of Sr and Ni were highly increased in NaP zeolite. In addition, the cation exchange capacity (CEC) experiment showed that NaP zeolite has an enhanced CEC of 310.89 cmol/kg compared to natural zeolite (CEC = 119.19 cmol/kg). In the actual batch sorption test, NaP zeolite (35.3 mg/g) still showed high Cs removal efficiency though it was slightly lower than the natural zeolite (39.0 mg/g). However, in case of Sr and Ni, NaP zeolite (27.9 and 27.8 mg/g, respectively) showed a much higher removal efficiency than natural zeolite (4.9 and 5.5 mg/g for Sr and Ni, respectively). This suggests that NaP zeolite, synthesized by a top-down desilication method, is more practical to remove mixed radionuclides from a waste solution.
Lithogeochemical and Hyperspectral Halos to Ag-Zn-Au Mineralization at Nimbus in the Eastern Goldfields Superterrane, Western Australia
Authors: Hollis Steven P. , Foury Sabri , Caruso Stefano , Johnson Sean , Barrote Vitor , Pumphrey Andrew
Published: March 2021

With new advances in rapid-acquisition geochemical and hyperspectral techniques, exploration companies are now able to detect subtle halos surrounding orebodies at minimal expense. The Nimbus Ag-Zn-(Au) deposit is unique in the Archean Yilgarn Craton of Western Australia. Due to its mineralogy, alteration assemblages, geochemical affinity, and tectonic setting, it is interpreted to represent a shallow water (~650 mbsl) and low-temperature (<250 °C) volcanogenic massive sulfide (VMS) deposit with epithermal characteristics (i.e., a hybrid bimodal felsic deposit). We present a detailed paragenetic account of the Nimbus deposit, and establish lithogeochemical and hyperspectral halos to mineralization to aid exploration. Mineralization at Nimbus is characterized by early units of barren massive pyrite that replace glassy dacitic lavas, and underlying zones of polymetallic sulfides that replace autoclastic monomict dacite breccias. The latter are dominated by pyrite-sphalerite-galena, a diverse suite of Ag-Sb ± Pb ± As ± (Cu)-bearing sulfosalts, minor pyrrhotite, arsenopyrite, and rare chalcopyrite. The main sulfosalt suite is characterized by pyrargyrite, and Ag-rich varieties of boulangerite, tetrahedrite, and bournonite. Zones of sulfide mineralization in quartz-sericite(±carbonate)-altered dacite are marked by significant mass gains in Fe, S, Zn, Pb, Sb, Ag, As, Cd, Ni, Cu, Ba, Co, Cr, Tl, Bi, and Au. Basaltic rocks show reduced mass gains in most elements, with zones of intense quartz-chlorite-carbonate±fuchsite alteration restricted to thick sequences of hyaloclastite, and near contacts with dacitic rocks. Broad zones of intense silica-sericite alteration surround mineralization in dacite, and are marked by high Alteration Index and Chlorite-Carbonate-Pyrite Index (CCPI) values, strong Na-Ca depletion, and an absence of feldspar (albite) in thermal infrared (TIR) data. White mica compositions are predominantly muscovitic in weakly altered sections of the dacitic footwall sequence. More paragonitic compositions are associated with zones of increased sericitization and high-grade polymetallic sulfide mineralization. Chlorite in dacitic rocks often occurs adjacent to zones of sulfide mineralization and is restricted to narrow intervals. Carbonate abundance is sporadic in dacite, but is most abundant outside the main zones of Na-Ca depletion. Basaltic rocks are characterized by strongly paragonitic white mica compositions, and abundant chlorite and carbonate. Shifts from Ca carbonates and Fe-rich chlorites to more Mg-rich compositions of both minerals occur in more intensely hydrothermally altered basaltic hyaloclastite, and near contacts with dacitic rocks. Hanging-wall polymict conglomerates are characterized by minor amounts of muscovitic to phengitic white mica (2205–2220 nm), and an absence of chlorite and carbonate alteration.
Processing Effect and Characterization of Olive Oils from Spanish Wild Olive Trees (Olea europaea var. sylvestris)
Authors: Espínola Francisco , Vidal Alfonso M. , Espínola Juan M. , Moya Manuel
Published: March 2021

Wild olive trees have important potential, but, to date, the oil from wild olives has not been studied significantly, especially from an analytical point of view. In Spain, the wild olive tree is called “Acebuche” and its fruit “Acebuchina”. The objective of this work is to optimize the olive oil production process from the Acebuchina cultivar and characterize the oil, which could be marketed as healthy and functional food. A Box–Behnken experimental design with five central points was used, along with the Response Surface Methodology to obtain a mathematical experimental model. The oils from the Acebuchina cultivar meet the requirements for human consumption and have a good balance of fatty acids. In addition, the oils are rich in antioxidants and volatile compounds. The highest extraction yield, 12.0 g oil/100 g paste, was obtained at 90.0 min and the highest yield of phenolic compounds, 870.0 mg/kg, was achieved at 40.0 °C, and 90.0 min; but the maximum content of volatile compounds, 26.9 mg/kg, was obtained at 20 °C and 30.0 min. The oil yield is lower than that of commercial cultivars, but the contents of volatile and phenolic compounds is higher.
Biochemical Properties of Tyrosinase from Aspergillus terreus and Penicillium copticola; Undecanoic Acid from Aspergillus flavus, an Endophyte of Moringa oleifera, Is a Novel Potent Tyrosinase Inhibitor
Authors: Salah Maamoun Hanaa , Rabie Gamal H. , Shaker Ibrahim , A. Alaidaroos Bothaina , El-Sayed Ashraf S. A.
Published: March 2021

Tyrosinase is a copper-containing monooxygenase catalyzing the O-hydroxylation of tyrosine to 3,4-dihydroxyphenylalanine then to dopaquinone that is profoundly involved in melanin synthesis in eukaryotes. Overactivation of tyrosinase is correlated with hyperpigmentation that is metabolically correlated with severe pathological disorders, so, inhibition of this enzyme is the most effective approach in controlling the overproduction of melanin and its hazardous effects. Thus, searching for a powerful, selective inhibitor of human tyrosinase to limit the hyper-synthesis of melanin is a challenge. Unlike the difficulty of overexpression of human tyrosinase, using fungal tyrosinase as a model enzyme to the human one to evaluate the mechanistics of enzyme inhibition in response to various compounds is the most feasible strategy. Thus, the purification of highly catalytic-efficient fungal tyrosinase, exploring a novel inhibitor, and evaluating the mechanistics of enzyme inhibition are the main objectives of this work. Aspergillus terreus and Penicillium copticola were reported as the most potential tyrosinase producers. The biochemical properties suggest that this enzyme displays a higher structural and catalytic proximity to human tyrosinase. Upon nutritional bioprocessing by Plackett–Burman design, the yield of tyrosinase was increased by about 7.5-folds, compared to the control. The purified tyrosinase was strongly inhibited by kojic acid and A. flavus DCM extracts with IC50 values of 15.1 and 12.6 µg/mL, respectively. From the spectroscopic analysis, the main anti-tyrosinase compounds of A. flavus extract was resolved, and verified as undecanoic acid. Further studies are ongoing to unravel the in vivo effect and cytotoxicity of this compound in fungi and human, that could be a novel drug to various diseases associated with hyperpigmentation by melanin.
Antiproliferative Isoprenoid Derivatives from the Red Sea Alcyonacean Xenia umbellata
Authors: Althagbi Hanan I. , Budiyanto Fitri , Abdel-Lateff Ahmed , Al-Footy Khalid O. , Bawakid Nahed O. , Ghandourah Mohamed A. , Alfaifi Mohammad Y. , Elbehairi Serag Eldin I. , Alarif Walied M.
Published: March 2021

From the soft coral Xenia umbellata, seven isoprenoid derivatives were isolated, including a new xenicane diterpene, xeniolide O (5) and a new gorgostane derivative gorgst-3β,5α,6β,11α,20(S)-pentol-3-monoacetate (7), along with three known sesquiterpenes (1–3), a known diterpene (4), and a known steroid (6). The extensive analyses of the NMR, IR, and MS spectral data led to determination of their chemical structures. Compounds 1–7 displayed a cytotoxic effect against breast adenocarcinoma (MCF-7), hepatocellular carcinoma (HepG2), and cervix adenocarcinoma (HeLa), with IC50 values ranging between 1.5 ± 0.1–23.2 ± 1.5; 1.8 ± 0.1–30.6 ± 1.1 and 0.9 ± 0.05–12.8 ± 0.5 μg/mL, respectively. Compound 3 showed potent cytotoxic effects against MCF-7, HepG2, and HeLa with IC50 values = 2.4 ± 0.20, 3.1 ± 0.10 and 0.9 ± 0.05 μg/mL, respectively. Compounds 2, 5, and 7 displayed cytotoxic effect against Hela cells with IC50 values = 12.8 ± 0.50, 6.7 ± 1.00 and 11.5 ± 2.20 μg/mL, respectively. Two DNA binding dyes, acridine orange (AO) and ethidium bromide (EtBr) were used for the detection of viable, apoptotic, and necrotic cells. The early apoptotic cell death was observed in all types of treated cells. The late apoptotic cells were highly present in HepG2 cells. Compounds 5 and 7 induced a high percentage of necrosis towards HepG2 and HeLa cells. The late apoptosis was recorded as a high rate after treatment with 7 on all cancer cells.
Gold Inks for Inkjet Printing on Photo Paper: Complementary Characterisation
Authors: Tiyyagura Hanuma Reddy , Majerič Peter , Bračič Matej , Anžel Ivan , Rudolf Rebeka
Published: March 2021

Nowadays, cost-effective, available, and flexible paper-based electronics play an essential role in the electronics industry. Herein, we present gold nanoparticles (AuNPs) as a potential raw material for gold inks in the future for such purposes. AuNPs in this research were synthesised using the ultrasonic spray pyrolysis (USP) technique from two precursors: gold (III) chloride tetrahydrate and gold (III) acetate. Synthesised AuNPs were collected in a suspension composed of deionised (D.I.) water and the stabiliser polyvinylpyrrolidone (PVP). AuNPs’ suspensions were subjected to the rotavapor process to obtain gold inks with higher Au concentration (>300 ppm). ICP-MS measurements, the size and shape of AuNPs, ζ-potential, Ultraviolet-visible (UV-Vis) spectrophotometry measurements, and scanning electron microscop y (SEM) of gold inks were carried out in order to find the optimal printing parameters. In the final stage, the optical contact angle measurements were performed using a set of polar to non-polar liquids, allowing for the determination of the surface free energy of gold inks. Inkjet printing of gold inks as defined stripes on photo paper were tested, based on the characterisation results.
Derivation of Luminescent Mesoporous Silicon Nanocrystals from Biomass Rice Husks by Facile Magnesiothermic Reduction
Authors: Sekar Sankar , Lee Sejoon
Published: March 2021

High-quality silicon (Si) nanocrystals that simultaneously had superior mesoporous and luminescent characteristics were derived from sticky, red, and brown rice husks via the facile and cost-effective magnesiothermic reduction method. The Si nanocrystals were confirmed to comprise an aggregated morphology with spherical nanocrystals (e.g., average sizes of 15–50 nm). Due to the surface functional groups formed at the nanocrystalline Si surfaces, the Si nanocrystals clearly exhibited multiple luminescence peaks in visible-wavelength regions (i.e., blue, green, and yellow light). Among the synthesized Si nanocrystals, additionally, the brown rice husk (BRH)-derived Si nanocrystals showed to have a strong UV absorption and a high porosity (i.e., large specific surface area: 265.6 m2/g, small average pore diameter: 1.91 nm, and large total pore volume: 0.5389 cm3/g). These are indicative of the excellent optical and textural characteristics of the BRH-derived Si nanocrystals, compared to previously reported biomass-derived Si nanocrystals. The results suggest that the biomass BRH-derived Si nanocrystals hold great potential as an active source material for optoelectronic devices as well as a highly efficient catalyst or photocatalyst for energy conversion devices.
Comparative Analysis of Proteins Regulated during Cadmium Sulfide Quantum Dots Response in Arabidopsis thaliana Wild Type and Tolerant Mutants
Authors: Gallo Valentina , Zappettini Andrea , Villani Marco , Marmiroli Nelson , Marmiroli Marta
Published: March 2021

In previous work, two independent Arabidopsis thaliana Ac/Ds transposon insertional mutant lines, atnp01 and atnp02, were identified that showed a higher level of tolerance than the wild type (wt) line to cadmium sulfide quantum dots (CdS QDs). The tolerance response was characterized at physiological, genetic and transcriptomic levels. In this work, a comparative analysis was performed on protein extracts from plantlets of the two mutants and of wt, each treated with 80 mg L−1 CdS QDs. A comparative protein analysis was performed by 2D-PAGE, and proteins were characterized by MALDI-TOF/TOF mass spectrometry. Of 250 proteins identified from all three lines, 98 showed significant changes in relative abundance between control and CdS QD-treated plantlets. The wt, atnp01, and atnp02 control-treated pairs respectively showed 61, 31, and 31 proteins with differential expression. The two mutants had a different response to treatment in terms of type and quantity of up- and downregulated proteins. This difference became more striking when compared to wt. A network analysis of the proteins differentially expressed in atnp01 and atnp02 included several of those encoded by putative genes accommodating the transposons, which were responsible for regulation of some proteins identified in this study. These included nifu-like protein 3 (Nfu3), involved in chloroplast assembly, elongator complex 3 (Elo3), involved in transcriptional elongation, magnesium-chelate subunit-2 (Chli2), involved in chlorophyll biosynthesis, and protein phosphatase 2C (PP2C) which mediates abiotic stress response.
A Log Ratio-Based Analysis of Individual Changes in the Composition of the Oral Microbiota in Different Dietary Phases
Authors: Vach Kirstin , Al-Ahmad Ali , Anderson Annette , Woelber Johan Peter , Karygianni Lamprini , Wittmer Annette , Hellwig Elmar
Published: March 2021

Background: Investigating the influence of nutrition on oral health has a long scientific history. Due to recent technical advances like sequencing techniques for the oral microbiota, this topic has gained scientific interest again. A basic challenge is to understand the influence of nutrition on the oral microbiota and on the interaction between the oral bacteria, which is also statistically challenging. Methods: Log-transformed ratios of two bacteria concentrations are introduced as the basic analytic tool. The framework is illustrated by application in an experimental study exposing eleven participants to different nutrition schemes in five consecutive phases. Results: The method could be sufficiently used to analyse the interrelation between the bacteria and to identify some bacterial groups with the same as well as different reactions to additional dietary components. It was found that the strongest changes in bacterial concentrations were achieved by the additional consumption of dairy products. Conclusion: A log ratio-based analysis offers insights into the relation of different bacteria while taking specific features of compositional data into account. The presented methods allow becoming independent of the behaviour of other bacteria, which is a disadvantage of common analysis methods of compositions. The results indicate that modulations of the oral biofilm microbiota due to nutrition change can be attained.
Coconut Oil Supplementation Does Not Affect Blood Pressure Variability and Oxidative Stress: A Placebo-Controlled Clinical Study in Stage-1 Hypertensive Patients
Authors: Júnior Francisco A. O. , Ruiz Clara R. , de Oliveira Yohanna , Barros Marco A. V. , Silva Alexandre S. , Santos Maria S. B. , Martins Vinícius J. B. , Balarini Camille M. , Braga Valdir A.
Published: March 2021

Exploring an alternative to improve the clinical management of hypertension, we tested the hypothesis that food supplementation with coconut oil (EVCO), alone or combined with aerobic exercise training, could exert an antihypertensive effect (primary outcome) in patients with stage 1 hypertension. Forty-five hypertensive volunteers of both genders participated in a placebo-controlled clinical trial. The volunteers were submitted to 24-hour ambulatory blood pressure monitoring, analysis of blood pressure variability (BPV), measurement of serum malondialdehyde (MDA) and nutritional assessment. Results indicate that EVCO consumption had no adverse effects. The supplementation did not increase the caloric intake compared with placebo, and the dietary constituents were similar between groups, except for the saturated fats, especially lauric acid. The analysis of blood pressure indicated absence of antihypertensive effect of EVCO alone or combined with physical training. Furthermore, no effects on blood pressure variability and oxidative stress were observed in the supplemented hypertensive patients. Thus, despite the results observed in pre-clinical studies, the current clinical study did not provide evidence to support the use of coconut oil as an adjuvant in the management of hypertension in humans.
Implementing a Transition Program from Paediatric to Adult Services in Phenylketonuria: Results after Two Years of Follow-Up with an Adult Team
Authors: Peres Maria , Almeida Manuela F. , Pinto Élia J. , Carmona Carla , Rocha Sara , Guimas Arlindo , Ribeiro Rosa , Martins Esmeralda , Bandeira Anabela , MacDonald Anita , Rocha Júlio C.
Published: March 2021

We aimed to report the implementation of a phenylketonuria (PKU) transition program and study the effects of follow-up with an adult team on metabolic control, adherence, and loss of follow-up. Fifty-five PKU patients were analysed in the study periods (SP): 2 years before (SP1) and after the beginning of adult care (SP2). Retrospective data on metabolic control and number of clinic appointments were collected for each SP, and protein intakes were analysed. In SP2, three patients (6%) were lost to follow-up. There was a small but statistically significant increase in median number of annual blood spots from SP1 to SP2: 11 (7–15) vs. 14 (7–20); p = 0.002. Mean ± SD of median blood Phe remained stable (525 ± 248 µmol/L vs. 552 ± 225 µmol/L; p = 0.100); median % of blood Phe < 480 µmol/L decreased (51 (4–96)% vs. 37 (5–85)%; p = 0.041) and median number of clinic appointments increased from SP1 to SP2: (5 (4–6) vs. 11 (8–13); p < 0.001). No significant differences were found regarding any parameter of protein intake. Our results suggest that the implementation of an adult service was successful as impact on metabolic control was limited and attendance remained high. Continuous dietetic care likely contributed to these results by keeping patients in follow-up and committed to treatment.
Psychological Resilience, Experimentally Manipulated Social Status, and Dietary Intake among Adolescents
Authors: Guazzelli Williamson Victoria , Lee Alexandra M. , Miller Darci , Huo Tianyao , Maner Jon K. , Cardel Michelle
Published: March 2021

Relative to other racial/ethnic groups in the United States, Hispanic American (HA) youth have higher rates of overweight and obesity. Previous work suggests that low perceived social status (SS) promotes excess caloric intake and, thereby, development of obesity. Psychological resilience may play a role in reducing adverse eating behaviors and risk for obesity. The objective of this study was to investigate whether resilience (as measured by the Connor Davidson Resilience Scale) interacts with experimentally manipulated SS to affect dietary intake among HA adolescents (n = 132). Using a rigged game of Monopoly (Hasbro, Inc.), participants were randomized to a high or low SS condition. Following the Monopoly game, participants consumed an ad libitum lunch and their dietary intake was assessed. There was a significant interaction between resilience and experimentally manipulated SS for total energy intake (p = 0.006), percent energy needs consumed (p = 0.005), and sugar intake (p = 0.004). For the high SS condition, for each increase in resilience score, total energy intake decreased by 7.165 ± 2.866 kcal (p = 0.014) and percent energy needs consumed decreased by 0.394 ± 0.153 (p = 0.011). In the low SS condition, sugar intake increased by 0.621 ± 0.240 g for each increase in resilience score (p = 0.011). After correction for multiple comparisons, the aforementioned interactions, but not simple slopes, were statistically significant.
Iodine Absorption in Celiac Children: A Longitudinal Pilot Study
Authors: Delvecchio Maurizio , Bizzoco Francesca , Lapolla Rosa , Gentile Antonia , Carrozza Cinzia , Barone Michele , Simonetti Simonetta , Giordano Paola , Dargenio Vanessa Nadia , Cristofori Fernanda , Francavilla Ruggiero
Published: March 2021

Background: non-autoimmune thyroid disorder is a common finding in celiac patients, more frequent than in the general population. An impairment of iodine absorption has been hypothesized, but it has never been investigated so far. We aimed to evaluate the iodine absorption in children and adolescents with newly diagnosed celiac disease. Methods: 36 consecutive celiac patients (age 7.4 years, range 2.4–14.5 years) before starting a gluten-free diet (GFD) were enrolled. We assayed the urinary iodine concentration (UIC) in a 24-h urine sample, at baseline (T0) after 3 (T1) and 12 months (T2) of GFD. Results: UIC at T0 was 64 μg/L (IQR 45–93.25 μg/L) with an iodine deficiency rate of 77.8%. UIC was not different according to histological damage, clinical presentation (typical vs atypical); we found no correlation with the thyroid function tests and auxological parameters. UIC was not statistically different at T1 (76 μg/L) and T2 (89 μg/L) vs T0. UIC at T2 was similar between patients with positive and negative anti-transglutaminase antibodies at T2. No patients presented overt hypothyroidism during the study. Conclusions: We found that iodine absorption in celiac children is impaired compared to the general population; it increases slightly, but not significantly, during the GFD. We should regularly reinforce the need for a proper iodine intake in celiac disease patients to reduce iodine deficiency risk.
Vitamin K and D Supplementation and Bone Health in Chronic Kidney Disease—Apart or Together?
Authors: Ziemińska Marta , Sieklucka Beata , Pawlak Krystyna
Published: March 2021

Vitamin K (VK) and vitamin D (VD) deficiency/insufficiency is a common feature of chronic kidney disease (CKD), leading to impaired bone quality and a higher risk of fractures. CKD patients, with disturbances in VK and VD metabolism, do not have sufficient levels of these vitamins for maintaining normal bone formation and mineralization. So far, there has been no consensus on what serum VK and VD levels can be considered sufficient in this particular population. Moreover, there are no clear guidelines how supplementation of these vitamins should be carried out in the course of CKD. Based on the existing results of preclinical studies and clinical evidence, this review intends to discuss the effect of VK and VD on bone remodeling in CKD. Although the mechanisms of action and the effects of these vitamins on bone are distinct, we try to find evidence for synergy between them in relation to bone metabolism, to answer the question of whether combined supplementation of VK and VD will be more beneficial for bone health in the CKD population than administering each of these vitamins separately.