bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification and Tissue-Specific Expression Analysis of CYP720B Subfamily Genes in Slash Pine and Loblolly Pine
Authors: Zhang Yini , Ding Xianyin , Luan Qifu , Jiang Jingmin , Diao Shu
Published: February 2022

Diterpene resin acids (DRAs) are major components of pine oleoresin that can effectively resist the invasion of insects and pathogenic microorganisms. The subfamily of cytochrome P450s, CYP720B, catalyzes diterpene products into DRAs. Identifying CYP720B subfamily members and revealing the characteristics of tissue-specific expression would help understand diterpene-rich structures and diverse types. Slash pine and loblolly pine are important pines that provide oleoresin products. In this study, we identified CYP720B candidate genes based on the Pinus taeda V2.0 genome and full-length transcriptome of slash pine by PacBio. A total of 17 genes in slash pine and 19 in loblolly pine were identified and classified into four main clades by phylogenetic analysis. An analysis of cis-acting elements showed that CYP720B genes were closely related to adversity resistance. The gene expression of these candidates in different tissues was quantified by real-time quantitative PCR (RT–qPCR) analysis. Most of the genes showed relatively higher expression levels in roots and stems than in the other tissues, corresponding with the results of DRA component detection by gas chromatography–mass spectrometry (GC–MS), which indicated that stems and roots might be important tissues in oleoresin biosynthesis. These results provide a valuable resource for a better understanding of the biological role of individual CYP720Bs in slash pine and loblolly pine.
Effect of Fibre Diameter, Prickle Factor and Coarse Fibre Bias on Yarn Surface Hairiness in South American Camelids (SAC) Fibre
Authors: Mamani-Cato Ruben Herberht , Frank Eduardo Narciso , Prieto Alejandro , Castillo Maria Flavia , Condori-Rojas Nicoll , Hick Michel Victor Hubert
Published: February 2022

It is well known that objectionable fibres emerge from the surface of the yarn due to the centrifugal force of the spinning device. Furthermore, the hair removal process is based on the same physical principles. However, the fibres that are >30 µm (PcF) are the fibres that appear in the hairiness of the yarn and are eliminated by dehairing. It has always been presumed that the PcF was linearly correlated with the diameter of the fibre (MFD) in llamas, but not so in alpaca fibres. Nevertheless, there is evidence that this relationship is curvilinear and behaves the same way in both species. The objectives of this study are to explore the relationship between MFD and PcF in both llamas and alpacas, to explore the existence of a breaking point (BP) in this curvilinear relationship, and to determine the frequency of fleeces that do not require dehairing because the PcF ≤ 3.2%. In addition, the existence of a positive bias of coarse fibre content on the hairy surface (CFs) of the yarn to coarse fibre content within the yarn fibres (CFy) was determined, which may explain the effect of the dehairing on the prickle factor of SAC fibres. The relationship of PcF on MFD behaves the same way in alpacas and llamas. It conforms to a power distribution and presents a BP of 23 µm, with PcF being constant before the BP and increasing significantly after it. Most animals (≤91% of alpacas and ≤87% of llamas) are above the threshold (≤3.2%), requiring dehairing to correct it. By means of a shaving technique on the surface of the fabric sample, it was established that the objectionable CFs content is 8.15% higher than the objectionable CFy content. In the evoked-coarse fibre in the dehaired samples, a CFs-CFy difference below 5.9% (p > 0.05) is not significantly detected by panellists. The surface MFD is more than 2.7 µm coarser than the yarn MFD.
Acknowledgment to Reviewers of Fibers in 2021
Authors:   Fibers Editorial Office
Published: February 2022

Rigorous peer-reviews are the basis of high-quality academic publishing [...]
Comparison of Self-Report Questionnaire and Eye Tracking Method in the Visual Preference Study of a Youth–Beverage Model
Authors: Sun Hongbo , Wang Wanxin , Liu Xinnan , Zhu Benzhong , Huang Yue , Leng Xiaojing , Jia Lu
Published: February 2022

This study compares the characteristics of a self-report questionnaire (SRQ) and eye tracking (ET) based on a simple human–beverage visual cognition model. The young participants were mainly defined by their gender and body mass index (BMI). The beverage samples consisted of milk, coffee, cup, and coaster. SRQs allow the participants to clearly express their overall cognition of the samples in the form of vocabulary, while ET captures their hidden thinking process. The analysis, using a random forest (RF) classifier, found that participant parameters (gender and BMI) played a more important role for SRQ, while ET was related to beverage parameters (color and shape). This work reiterates that these two methods have their advantages and complement each other in food sensory analysis.
Volatile Composition and Sensory Characterization of Dry White Wines Made with Overripe Grapes by Means of Two Different Techniques
Authors: Sancho-Galán Pau , Amores-Arrocha Antonio , Palacios Víctor , Jiménez-Cantizano Ana
Published: February 2022

Grape over-ripening is a technique that has historically been used for the production of white wines in southern Spain. However, this technique is still widely used for the production of sweet wines. In this study and after recently proving the feasibility of making dry white wines from overripe grapes with and without the presence of grape skin in a warm climate zone, the sensory characterization and analysis of the major and minor volatile compounds in dry white wines made from overripe grapes are presented for the first time. Two over-ripening techniques (sun-drying and climatic chamber drying) were studied for two different periods of time (48 and 96 h), as has the presence of grape skins during alcoholic fermentation. Grape over-ripening implies modifications in the composition of both the major and minor volatile compounds in wines. In terms of sensory analysis, wines with a similar profile were obtained year-on-year. The results of the preference test show that the wines made from grapes that had been over-ripened in the sun for 96 h were preferred by the tasting panel for both vintages. Thus, grape over-ripening under the sun could be considered as a resilience and adaptation technique for increased temperature conditions during the ripening season caused by the effects of climate change.
Cleaning and Disinfection Protocols for Clear Orthodontic Aligners: A Systematic Review
Authors: Charavet Carole , Gourdain Zoé , Graveline Léa , Lupi Laurence
Published: February 2022

(1) Background: Clear orthodontic aligners support the development of oral biofilms, which could lead to interferences with the oral microbiota already existing and the deterioration of oral health, with the development of dental caries, periodontal disease and even systemic infections. Therefore, preventive oral health care requires a cleaning and disinfection procedure for aligners. (2) Methods: A systematic review of the literature was conducted across four databases following the PRISMA guidelines up to May 2021, combining an electronic and a manual search. Prospective studies, including randomized controlled trials (RCTs), crossover studies (COSs) and controlled clinical trials (CCTs), published in the English language without time restrictions, evaluating the efficacy of cleaning and disinfection protocols for clear orthodontic aligners by comparing them with a placebo or a negative control, were included. The article selection, data extraction and risk of bias assessment were performed by two independent blinded review authors. In case of disagreement, a third author was solicited throughout the selection process. (3) Results: Among the 221 articles screened in the search process, 4 studies were included in the review, all designed as crossover studies (single arm without randomization with the same sequence of different cleaning and disinfection protocols for each participant). Different cleaning and disinfection methods were studied such as mechanical methods (brushing with toothpaste or vibration), chemical methods/pharmaceutical products (chlorhexidine antibacterial substance, anionic or cationic detergents or effervescent tablets) or combinations of both. (4) Conclusion: Although the determination of the most remarkable method of cleaning and disinfection was impossible because no direct comparison was conducted between all these methods, a multi-step protocol, including the combination of a mechanical and a chemical method, seems to be the most effective approach. Further research is needed to define the most preventive oral health care protocol. Registration: PROSPERO CRD 42021278498.
A Pathfinding Algorithm for Lowering Infection Exposure of Healthcare Personnel Working in Makeshift Hospitals
Authors: Rolle Braxton , Kiran Ravi , Straub Jeremy
Published: February 2022

Due to the recent COVID-19 outbreak, makeshift (MS) hospitals have become an important feature in healthcare systems worldwide. Healthcare personnel (HCP) need to be able to navigate quickly, effectively, and safely to help patients, while still maintaining their own well-being. In this study, a pathfinding algorithm to help HCP navigate through a hospital safely and effectively is developed and verified. Tests are run using a discretized 2D grid as a representation of an MS hospital plan, and total distance traveled and total exposure to disease are measured. The influence of the size of the 2D grid units, the shape of these units, and degrees of freedom in the potential movement of the HCP are investigated. The algorithms developed are designed to be used in MS hospitals where airborne illness is prevalent and could greatly reduce the risk of illness in HCP. In this study, it was found that the quantum-based algorithm would generate paths that accrued 50–66% less total disease quantum than the shortest path algorithm with also about a 33–50% increase in total distance traveled. It was also found that the mixed path algorithm-generated paths accrued 33–50% less quantum, but only increased total distance traveled by 10–20%.
Determination of Phylloplane Associated Bacteria of Lettuce from a Small-Scale Aquaponic System via 16S rRNA Gene Amplicon Sequence Analysis
Authors: Kasozi Nasser , Kaiser Horst , Wilhelmi Brendan
Published: February 2022

Fresh vegetables harbour diverse bacterial populations on their surfaces which are important for plant health and growth. Information on epiphytic bacteria is limited to only a few types of vegetables and it is unknown how the lettuce epiphytic bacterial community structure may respond when a probiotic product is added to an aquaponic system. In this study, we evaluated lettuce growth and analysed epiphytic bacterial communities of lettuce based on metabarcoding analysis of the V3-V4 region of the 16S rRNA gene obtained from paired-end Illumina MiSeq reads. The addition of Bacillus probiotics resulted in a significant increase of nitrate and phosphate in the deep-water culture solution, as well as increased vegetative growth of lettuce. Metabarcoding analysis revealed that the most abundant phyla on lettuce leaf surfaces were Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria. The in-depth bacterial composition analysis indicated that genera Chryseobacterium, Bacillus, Pantoea, Pseudoduganella, Flavobacterium, Paludibacter, and Cloacibacterium were dominant in leaf samples obtained from Bacillus-treated systems. Analysis of lettuce epiphytic bacterial communities of the fresh lettuce leaf surfaces also indicated the presence of food-borne pathogens belonging to the Shigella and Aeromonas genera, which were less abundant in the probiotic treated systems. This study provides the first characterization of the epiphytic bacterial community structure and how it can be modulated by the addition of a probiotic mixture to the nutrient solution of aquaponic systems.
Examining the Associations between COVID-19-Related Psychological Distress, Social Media Addiction, COVID-19-Related Burnout, and Depression among School Principals and Teachers through Structural Equation Modeling
Authors: Karakose Turgut , Yirci Ramazan , Papadakis Stamatis
Published: February 2022

This study aims to investigate the relationships between COVID-19-related psychological distress, social media addiction, COVID-19-related burnout, and depression. The research, which was designed according to the relational survey model, was conducted with the participation of 332 school principals and teachers who received graduate education in the field of educational administration. Research data were collected through online surveys and then structural equation modeling (SEM) was used to test and analyze the proposed hypotheses. The study’s findings revealed that COVID-19-related psychological distress strongly predicted COVID-19-related burnout. In this context, as the psychological distress associated with COVID-19 increased, the sense of burnout associated with COVID-19 also increased. However, it was found that burnout associated with COVID-19 significantly and positively predicted depression. SEM results revealed that COVID-19-related psychological distress directly affected COVID-19-related burnout, depression, and social media addiction. In addition, it was determined that an indirect effect of COVID-19-related burnout and social media addiction exists in the relationship between COVID-19-related psychological distress and depression.
Effects of Perceived Benefit on Vitamin D Supplementation Intention: A Theory of Planned Behaviour Perspective
Authors: Chen Ying-Hsuan , Chao Shun-Lung , Chu Yen-Wei
Published: February 2022

There are many factors that affect vitamin D supplementation, including those from the theory of planned behaviour (TPB); however, how the perceived benefit acts in the model remains unknown. In the current study, we tested the efficacy of the TPB and the impacts of the perceived benefit (PBE) in the model. The subjects were 287 customers who purchased vitD from pharmacies in major cities in Taiwan. A structured questionnaire was used to collect the data. t-tests, analysis of variance (ANOVA), regression analyses, and path analysis via SPSS and AMOS were used to analyse the data. The original TPB model explained 47.5% of the variance of intention with the three variables of attitude (β = 0.261), perceived behavioural control (β = 0.183), and subjective norms (β = 0.169). The model that incorporated PBE increased the explained variance to 59.7%, and PBE became the strongest predictor (β = 0.310) and a significant mediator linking attitude, subjective norms, perceived control (ANC) with supplementation intention. PBE and attitude were the two most important variables in predicting vitD supplementation intention. We suggest that updated information regarding dietary sources of vitD and its benefits should be included in health- or nutrition-related courses in education programs for the overall health of the nation.
A Cross-Sectional Study of Exposure Factors Associated with Seropositivity for SARS-CoV-2 Antibodies during the Second Epidemic Wave among a Sample of the University of Corsica (France)
Authors: Decarreaux Dorine , Sevila Julie , Masse Shirley , Capai Lisandru , Fourié Toscane , Saba Villarroel Paola Mariela , Amroun Abdennour , Nurtop Elif , Vareille Matthieu , Pouquet Marie , Blanchon Thierry , de Lamballerie Xavier , Charrel Rémi , Falchi Alessandra
Published: February 2022

This study aimed to estimate the seroprevalence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection within the staff and student populations of the University of Corsica (France) during the second wave of the epidemic. Methods: A cross-sectional survey was conducted from 23 November 2020 to 31 January 2021. The participants underwent blood sampling using a fingerstick procedure and completed an anonymized questionnaire. Sera were tested for the presence of anti-SARS-CoV-2 IgG (ELISA-S) and, if positive, with an in-house virus neutralization test (VNT). Results: A total of 418 persons were included in the study. The overall seroprevalence was 12.8% (95% confidence interval (CI), 9.8–16.6%). A total of 15 (31%) of the 49 individuals who had a positive ELISA-S also had a positive VNT. Seropositivity was associated with living at the city campus during the week and on weekends (OR = 3.74 [1.40–12.00]), using public transportation/carpooling (OR = 2.00 [1.01–4.02]), and being in contact with a person who tested positive for SARS-CoV-2 (OR = 2.32 [1.20–4.40]). The main symptoms associated with seropositivity were “having had an acute respiratory infection” (OR = 3.05 [1.43–6.43]) and “experiencing loss of smell” (OR = 16.4 [5.87–50.7]). Conclusion: These results could be useful for SARS-CoV-2 prevention and control on university campuses.
Sleep Duration, Number of Awakenings and Arterial Stiffness in Industrial Shift Workers: A Five-Week Follow-Up Study
Authors: Matre Dagfinn , Sirnes Per Anton , Goffeng Elisabeth , Skare Øivind , Skogstad Marit
Published: February 2022

Shift work may increase the risk for hypertension and arterial stiffness, potentially a consequence of disturbed sleep. The aim of this study was to investigate possible correlations between sleep length and spontaneous awakenings with selected cardiovascular risk factors in shift workers at an industrial plant. We examined 19 shift workers by means of blood pressure and arterial stiffness, measured as pulse wave velocity (PWV), prior to and after a 5-week shift period. Sleep patterns were monitored on a daily basis with the assistance of a smartphone-based sleep diary (the entire test period) and by actigraphy (limited to 2 weeks). The number of awakenings and total sleep time were calculated. Shorter sleep duration was associated with higher blood pressure and partly with higher PWV, indicating an increased risk of cardiovascular disease (CVD) with reduced sleep duration. Unexpectedly, a lower number of awakenings was associated with an increase in blood pressure, indicating a reduced risk of CVD. No other significant associations were determined. The results from the present study among shift workers in Norway could support the hypothesis that short sleep duration is associated with elevated blood pressure and arterial stiffness.
The Impact of the COVID-19 Pandemic on Israeli Orthodontic Practice: A Clinic’s Activity and Patients’ Attitudes
Authors: Sella Tunis Tatiana , Ratson Tal , Matalon Shlomo , Abba Michael , Abramson Alex , Davidovitch Moshe , Shpack Nir
Published: February 2022

The current study aimed to characterize the activity in orthodontic clinics during the COVID-19-induced lockdown and the inter-lockdown periods, as well as to evaluate patients’ perspectives with respect to their fears, their extent of cooperation with treatment, and their emergency needs during the lockdown. The data were gathered from 11 private orthodontic clinics from 1 January 2020 to 8 March 2021, which included three lockdowns and inter-lockdowns. Information specifying the number of admissions, missed appointments, and emergency visits was gathered. Four hundred and twenty-nine orthodontic patients treated in those clinics agreed to complete a questionnaire that evaluated their concerns and expectations, the treatment emergency issues, implementation of the orthodontist’s instructions, and contact with the clinical staff during lockdowns. There was a significant increase in the number of scheduled appointments during the inter-lockdown periods, compared with the pre-pandemic period (p = 0.001). No difference in the number of missed/canceled or emergency appointments was found between the different periods (p > 0.420). The majority (89.6%) of the emergency visits involved issues with appliances; 68.7% of the subjects were advised to present themselves at clinics. During the peaks of the waves of the COVID-19 pandemic, a sharp rise in the number of missed and urgent appointments was not found. Reducing the number of orthodontic emergencies may assist in reducing patient fears.
Prenatal Mercury Exposure and Neurodevelopment up to the Age of 5 Years: A Systematic Review
Authors: Dack Kyle , Fell Matthew , Taylor Caroline M. , Havdahl Alexandra , Lewis Sarah J.
Published: February 2022

Neurodevelopmental delays can interfere with children’s engagement with the world and further development, and may have negative consequences into adulthood. Mercury is highly toxic and may negatively influence neurodevelopment because it can freely cross the placenta and accumulate in the fetal brain. We searched four publication databases (Embase, PsycINFO, PubMed/MEDLINE, Scopus) for studies examining the relationship between early life mercury exposure and scores on neurodevelopmental performance measures in children aged 0 to 5 years old. Study quality was assessed using the National Institutes of Health (NIH) Quality Assessment Tool. Thirty-two prospective studies were included in the review. Neurodevelopmental performance was measured using 23 different scales, most commonly the Bayley Scales of Infant and Toddler Development (BSID). In most cases, the evidence for an association between mercury and neurodevelopment was weak. There did not appear to be exceptions for particular childhood ages, outcome scales, or mercury levels. The small number of results to the contrary were more likely to be studies which did not meet our high-quality criteria, and could be a consequence of multiple testing, selection bias, or incomplete confounder adjustment. Based on current evidence, dietary mercury exposure during pregnancy is unlikely to be a risk factor for low neurodevelopmental functioning in early childhood.
Using Behavior Change Interventions in Cardiac and Pulmonary Rehabilitation: Perspectives from Healthcare Professionals in the United Kingdom
Authors: Whittaker Eleanor M. , Levy Andrew R. , Matata Bashir , Kinnafick Florence E. , Midgley Adrian W.
Published: February 2022

This study explores healthcare professionals’ experiences of using behavior change interventions in clinical practice. Semi-structured qualitative interviews were conducted with 11 healthcare professionals working in a cardiac and pulmonary rehabilitation National Health Service Trust in the United Kingdom. Interviews were transcribed and analyzed using inductive thematic analysis. Four overarching themes representing healthcare practitioners’ perceptions of using behavior change interventions were identified: (1) reliance on experiential learning, (2) knowledge transition, (3) existing professional development programs, and (4) barriers and facilitators for continued professional development. The results are discussed in relation to the implications they may have for behavior change training in clinical healthcare practice. Healthcare professionals require bespoke and formalized training to optimize their delivery of behavior change interventions in cardiac and pulmonary rehabilitation. Doing so will enhance intervention fidelity and implementation that can potentially ameliorate patient rehabilitation outcomes.
Online Public Rumor Engagement Model and Intervention Strategy in Major Public Health Emergencies: From the Perspective of Social Psychological Stress
Authors: Liu Jiaqi , Qi Jiayin
Published: February 2022

During major public health emergencies, a series of coupling problems of rumors getting out of control and public psychological imbalance always emerge in social media, which bring great interference for crisis disposal. From the perspective of social psychological stress, it is important to depict the interactive infection law among distinct types of rumor engagers (i.e., advocates, supporters, and amplifiers) under different social psychological stress states, and explore the effectiveness of rumor intervention strategies (i.e., hindering and persuasion) from multiple dimensions, to scientifically predict the situation of public opinion field and guide the public to restore psychological stability. Therefore, this paper constructs an interactive infection model of multiple rumor engagers under different intervention situations based on a unique user-aggregated dataset collected from a Chinese leading online microblogging platform (“Sina Weibo”) during the COVID-19 in 2020. The simulation result shows that (1) in the period of social psychological alarm reaction, the strong level of hindering intervention on the rumor engagers leads to more serious negative consequences; (2) in the period of social psychological resistance, the persuasion and hindering strategies can both produce good outcomes, which can effectively reduce the overall scale of rumor supporters and amplifiers and shorten their survival time in social media; (3) in the period of social psychological exhaustion, rumor intervention strategies are not able to have a significant impact; (4) the greater the intensity of intervention, the more obvious the outcome. Experimental findings provide a solid research basis for enhancing social psychological stress outcomes and offer decision-making references to formulate the rumor combating scheme.
Molecular Hydrogen Positively Affects Physical and Respiratory Function in Acute Post-COVID-19 Patients: A New Perspective in Rehabilitation
Authors: Botek Michal , Krejčí Jakub , Valenta Michal , McKune Andrew , Sládečková Barbora , Konečný Petr , Klimešová Iva , Pastucha Dalibor
Published: February 2022

Molecular hydrogen (H2) is potentially a novel therapeutic gas for acute post-coronavirus disease 2019 (COVID-19) patients because it has antioxidative, anti-inflammatory, anti-apoptosis, and antifatigue properties. The aim of this study was to determine the effect of 14 days of H2 inhalation on the respiratory and physical fitness status of acute post-COVID-19 patients. This randomized, single-blind, placebo-controlled study included 26 males (44 ± 17 years) and 24 females (38 ± 12 years), who performed a 6-min walking test (6 MWT) and pulmonary function test, specifically forced vital capacity (FVC) and expiratory volume in the first second (FEV1). Symptomatic participants were recruited between 21 and 33 days after a positive polymerase chain reaction test. The experiment consisted of H2/placebo inhalation, 2 × 60 min/day for 14 days. Results showed that H2 therapy, compared with placebo, significantly increased 6 MWT distance by 64 ± 39 m, FVC by 0.19 ± 0.24 L, and, in FEV1, by 0.11 ± 0.28 L (all p ≤ 0.025). In conclusion, H2 inhalation had beneficial health effects in terms of improved physical and respiratory function in acute post-COVID-19 patients. Therefore, H2 inhalation may represent a safe, effective approach for accelerating early function restoration in post-COVID-19 patients.
Towards Money Market in General Equilibrium Framework
Authors: Trinh Truong Hong
Published: February 2022

This paper aims to integrate the money market into the structure of the economy. The microfoundation is the starting point to define the money market and the general equilibrium mechanism of the economy. On this basis, this research seeks a linking mechanism of the money market with economic activity in the general equilibrium framework. The relationships between money supply and national outcome, inflation, and price level are studied in three cases: full-employment equilibrium economy, steady-state equilibrium economy, and sticky-price equilibrium economy. The research result explains the interrelation and transmission mechanism between the money market and the general equilibrium of the economy. The paper provides the theoretical foundation for further research on the money market and monetary policies towards economic growth and macroeconomic stability.
Cold Atmospheric Plasma Does Not Affect Stellate Cells Phenotype in Pancreatic Cancer Tissue in Ovo
Authors: Privat-Maldonado Angela , Verloy Ruben , Cardenas Delahoz Edgar , Lin Abraham , Vanlanduit Steve , Smits Evelien , Bogaerts Annemie
Published: February 2022

Pancreatic ductal adenocarcinoma (PDAC) is a challenging neoplastic disease, mainly due to the development of resistance to radio- and chemotherapy. Cold atmospheric plasma (CAP) is an alternative technology that can eliminate cancer cells through oxidative damage, as shown in vitro, in ovo, and in vivo. However, how CAP affects the pancreatic stellate cells (PSCs), key players in the invasion and metastasis of PDAC, is poorly understood. This study aims to determine the effect of an anti-PDAC CAP treatment on PSCs tissue developed in ovo using mono- and co-cultures of RLT-PSC (PSCs) and Mia PaCa-2 cells (PDAC). We measured tissue reduction upon CAP treatment and mRNA expression of PSC activation markers and extracellular matrix (ECM) remodelling factors via qRT-PCR. Protein expression of selected markers was confirmed via immunohistochemistry. CAP inhibited growth in Mia PaCa-2 and co-cultured tissue, but its effectiveness was reduced in the latter, which correlates with reduced ki67 levels. CAP did not alter the mRNA expression of PSC activation and ECM remodelling markers. No changes in MMP2 and MMP9 expression were observed in RLT-PSCs, but small changes were observed in Mia PaCa-2 cells. Our findings support the ability of CAP to eliminate PDAC cells, without altering the PSCs.
Lycopene in the Prevention of Cardiovascular Diseases
Authors: Przybylska Sylwia , Tokarczyk Grzegorz
Published: February 2022

Cardiovascular diseases (CVDs) are the leading cause of human mortality worldwide. Oxidative stress and inflammation are pathophysiological processes involved in the development of CVD. That is why bioactive food ingredients, including lycopene, are so important in their prevention, which seems to be a compound increasingly promoted in the diet of people with cardiovascular problems. Lycopene present in tomatoes and tomato products is responsible not only for their red color but also for health-promoting properties. It is characterized by a high antioxidant potential, the highest among carotenoid pigments. Mainly for this reason, epidemiological studies show a number of favorable properties between the consumption of lycopene in the diet and a reduced risk of cardiovascular disease. While there is also some controversy in research into its protective effects on the cardiovascular system, growing evidence supports its beneficial role for the heart, endothelium, blood vessels, and health. The mechanisms of action of lycopene are now being discovered and may explain some of the contradictions observed in the literature. This review aims to present the current knowledge in recent years on the preventive role of lycopene cardiovascular disorders.
Improved Production of 5-Hydroxymethylfurfural in Acidic Deep Eutectic Solvents Using Microwave-Assisted Reactions
Authors: Morais Eduarda S. , Freire Mara G. , Freire Carmen S. R. , Silvestre Armando J. D.
Published: February 2022

Hydroxymethylfurfural (5-HMF) is a key platform chemical, essential for the production of other chemicals, as well as fuels. Despite its importance, the production methods applied so far still lack in sustainability. In this work, acidic deep eutectic solvents (DES), acting both as solvent and catalyst, were studied for the conversion of fructose into 5-HMF using microwave-assisted reactions. These solvents were screened and optimized by varying the hydrogen bond donor (HBD) and hydrogen bond acceptor (HBA). The bio-based solvent γ-valerolactone (GVL) was also applied as additive, leading to a boost in 5-HMF yield. Then, a response surface methodology was applied to further optimize operating conditions, such as reaction time, temperature and wt.% of added GVL. The highest 5-HMF yield attained, after optimization, was 82.4% at 130 °C, in 4 min of reaction time and with the addition of 10 wt.% of GVL. Moreover, a process for 5-HMF recovery and DES reuse was developed through the use of the bio-based solvent 2-methyltetrahydrofuran (2-Me-THF), allowing at least three cycles of 5-HMF production with minimal yield losses, while maintaining the purity of the isolated 5-HMF and the efficacy of the reaction media.
The Induced Expression of BPV E4 Gene in Equine Adult Dermal Fibroblast Cells as a Potential Model of Skin Sarcoid-like Neoplasia
Authors: Podstawski Przemysław , Samiec Marcin , Skrzyszowska Maria , Szmatoła Tomasz , Semik-Gurgul Ewelina , Ropka-Molik Katarzyna
Published: February 2022

The equine sarcoid is one of the most common neoplasias in the Equidae family. Despite the association of this tumor with the presence of bovine papillomavirus (BPV), the molecular mechanism of this lesion has not been fully understood. The transgenization of equine adult cutaneous fibroblast cells (ACFCs) was accomplished by nucleofection, followed by detection of molecular modifications using high-throughput NGS transcriptome sequencing. The results of the present study confirm that BPV-E4- and BPV-E1^E4-mediated nucleofection strategy significantly affected the transcriptomic alterations, leading to sarcoid-like neoplastic transformation of equine ACFCs. Furthermore, the results of the current investigation might contribute to the creation of in vitro biomedical models suitable for estimating the fates of molecular dedifferentiability and the epigenomic reprogrammability of BPV-E4 and BPV-E4^E1 transgenic equine ACFC-derived sarcoid-like cell nuclei in equine somatic cell-cloned embryos. Additionally, these in vitro models seem to be reliable for thoroughly recognizing molecular mechanisms that underlie not only oncogenic alterations in transcriptomic signatures, but also the etiopathogenesis of epidermal and dermal sarcoid-dependent neoplastic transformations in horses and other equids. For those reasons, the aforementioned transgenic models might be useful for devising clinical treatments in horses afflicted with sarcoid-related neoplasia of cutaneous and subcutaneous tissues.
Caffeine and MDMA (Ecstasy) Exacerbate ER Stress Triggered by Hyperthermia
Authors: Trychta Kathleen A. , Harvey Brandon K.
Published: February 2022

Drugs of abuse can cause local and systemic hyperthermia, a known trigger of endoplasmic reticulum (ER) stress and the unfolded protein response (UPR). Another trigger of ER stress and UPR is ER calcium depletion, which causes ER exodosis, the secretion of ER-resident proteins. In rodent models, club drugs such as 3,4-methylenedioxymethamphetamine (MDMA, ‘ecstasy’) can create hyperthermic conditions in the brain and cause toxicity that is affected by the environmental temperature and the presence of other drugs, such as caffeine. In human studies, MDMA stimulated an acute, dose-dependent increase in core body temperature, but an examination of caffeine and MDMA in combination remains a topic for clinical research. Here we examine the secretion of ER-resident proteins and activation of the UPR under combined exposure to MDMA and caffeine in a cellular model of hyperthermia. We show that hyperthermia triggers the secretion of normally ER-resident proteins, and that this aberrant protein secretion is potentiated by the presence of MDMA, caffeine, or a combination of the two drugs. Hyperthermia activates the UPR but the addition of MDMA or caffeine does not alter the canonical UPR gene expression despite the drug effects on ER exodosis of UPR-related proteins. One exception was increased BiP/GRP78 mRNA levels in MDMA-treated cells exposed to hyperthermia. These findings suggest that club drug use under hyperthermic conditions exacerbates disruption of ER proteostasis, contributing to cellular toxicity.
ADAM8-Dependent Extracellular Signaling in the Tumor Microenvironment Involves Regulated Release of Lipocalin 2 and MMP-9
Authors: Cook Lena , Sengelmann Marie , Winkler Birte , Nagl Constanze , Koch Sarah , Schlomann Uwe , Slater Emily P. , Miller Miles A. , von Strandmann Elke Pogge , Dörsam Bastian , Preußer Christian , Bartsch Jörg W.
Published: February 2022

The metalloprotease-disintegrin ADAM8 is critically involved in the progression of pancreatic cancer. Under malignant conditions, ADAM8 is highly expressed and could play an important role in cell–cell communication as expression has been observed in tumor and immune cells of the tumor microenvironment (TME) such as macrophages. To analyze the potential role of ADAM8 in the TME, ADAM8 knockout PDAC tumor cells were generated, and their release of extracellular vesicles (EVs) was analyzed. In EVs, ADAM8 is present as an active protease and associated with lipocalin 2 (LCN2) and matrix metalloprotease 9 (MMP-9) in an ADAM8-dependent manner, as ADAM8 KO cells show a lower abundance of LCN2 and MMP-9. Sorting of ADAM8 occurs independent of TSG101, even though ADAM8 contains the recognition motif PTAP for the ESCRTI protein TSG101 within the cytoplasmic domain (CD). When tumor cells were co-cultured with macrophages (THP-1 cells), expression of LCN2 and MMP-9 in ADAM8 KO cells was induced, suggesting that macrophage signaling can overcome ADAM8-dependent intracellular signaling in PDAC cells. In co-culture with macrophages, regulation of MMP-9 is independent of the M1/M2 polarization state, whereas LCN2 expression is preferentially affected by M1-like macrophages. From these data, we conclude that ADAM8 has a systemic effect in the tumor microenvironment, and its expression in distinct cell types has to be considered for ADAM8 targeting in tumors.
Prognostic Value of Machine Learning in Patients with Acute Myocardial Infarction
Authors: Xiao Changhu , Guo Yuan , Zhao Kaixuan , Liu Sha , He Nongyue , He Yi , Guo Shuhong , Chen Zhu
Published: February 2022

(1) Background: Patients with acute myocardial infarction (AMI) still experience many major adverse cardiovascular events (MACEs), including myocardial infarction, heart failure, kidney failure, coronary events, cerebrovascular events, and death. This retrospective study aims to assess the prognostic value of machine learning (ML) for the prediction of MACEs. (2) Methods: Five-hundred patients diagnosed with AMI and who had undergone successful percutaneous coronary intervention were included in the study. Logistic regression (LR) analysis was used to assess the relevance of MACEs and 24 selected clinical variables. Six ML models were developed with five-fold cross-validation in the training dataset and their ability to predict MACEs was compared to LR with the testing dataset. (3) Results: The MACE rate was calculated as 30.6% after a mean follow-up of 1.42 years. Killip classification (Killip IV vs. I class, odds ratio 4.386, 95% confidence interval 1.943–9.904), drug compliance (irregular vs. regular compliance, 3.06, 1.721–5.438), age (per year, 1.025, 1.006–1.044), and creatinine (1 µmol/L, 1.007, 1.002–1.012) and cholesterol levels (1 mmol/L, 0.708, 0.556–0.903) were independent predictors of MACEs. In the training dataset, the best performing model was the random forest (RDF) model with an area under the curve of (0.749, 0.644–0.853) and accuracy of (0.734, 0.647–0.820). In the testing dataset, the RDF showed the most significant survival difference (log-rank p = 0.017) in distinguishing patients with and without MACEs. (4) Conclusions: The RDF model has been identified as superior to other models for MACE prediction in this study. ML methods can be promising for improving optimal predictor selection and clinical outcomes in patients with AMI.
Early Complications of Radioisotope Therapy with Lutetium-177 and Yttrium-90 in Patients with Neuroendocrine Neoplasms—A Preliminary Study
Authors: Bober Barbara , Saracyn Marek , Zaręba Kornelia , Lubas Arkadiusz , Mazurkiewicz Paweł , Wilińska Ewelina , Kamiński Grzegorz
Published: February 2022

Neuroendocrine neoplasms (NENs) constitute a heterogenous group of tumors originating from neuroendocrine cells scattered throughout the body. Peptide Receptor Radionuclide Therapy (PRRT) is a treatment of choice of unresectable metastasized progressive and well-differentiated NENs. The aim of the study was to assess early bone marrow and kidney injury after administration of Lutetium-177 or Lutetium-177 combined with Yttrium-90. Thirty-one patients received treatment with [177Lu]Lu-DOTATATE with the activity of 7.4 GBq. Eleven patients received tandem treatment with [90Y]Y-DOTATATE with the activity of 1.85 GBq + [177Lu]Lu-DOTATATE with the activity of 1.85 GBq. After PRRT a significant decrease in leukocyte, neutrophil, and lymphocyte counts was noted. Tandem treatment demonstrated a more marked decrease in white blood cell count compared to Lutetium-177 therapy only. Conversely, no significant influence on glomerular filtration was found in this assessment. However, PRRT triggered acute renal tubule dysfunction, regardless of the treatment type. Regarding the acute complications, PRRT appeared to be a safe modality in the treatment of patients with NEN.
Movement Disorders Induced by SARS-CoV-2 Infection: Protocol for a Scoping Review
Authors: Rosca Elena Cecilia , Vastag Zsolt , Phokaewvarangkul Onanong , Sringean Jirada
Published: February 2022

Infections are a significant cause of movement disorders. The clinical manifestations of SARS-CoV-2 infection are variable, with up to one-third of patients developing neurologic complications, including movement disorders. This scoping review will lay out a comprehensive understanding of movement disorders induced by SARS-CoV-2 infection. We aim to investigate the epidemiology, clinical and paraclinical features, interventions, and diagnostic challenges in patients with different types of movement disorders in the context of SARS-CoV-2 infection. We will search three databases applying appropriate search terms. Inclusion and exclusion criteria are pre-defined; the data of eligible studies will be extracted in standardized forms. We will report the results following Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR). We will present information for clinicians and other healthcare professionals, policymakers, and public health researchers. In addition, the results of the present review may assist in the development and confirmation of inclusion criteria and research questions for further systematic review or meta-analysis, with more precise, narrower questions.
Towards a Connected Mobile Cataract Screening System: A Future Approach
Authors: Wan Zaki Wan Mimi Diyana , Abdul Mutalib Haliza , Ramlan Laily Azyan , Hussain Aini , Mustapha Aouache
Published: February 2022

Advances in computing and AI technology have promoted the development of connected health systems, indirectly influencing approaches to cataract treatment. In addition, thanks to the development of methods for cataract detection and grading using different imaging modalities, ophthalmologists can make diagnoses with significant objectivity. This paper aims to review the development and limitations of published methods for cataract detection and grading using different imaging modalities. Over the years, the proposed methods have shown significant improvement and reasonable effort towards automated cataract detection and grading systems that utilise various imaging modalities, such as optical coherence tomography (OCT), fundus, and slit-lamp images. However, more robust and fully automated cataract detection and grading systems are still needed. In addition, imaging modalities such as fundus, slit-lamps, and OCT images require medical equipment that is expensive and not portable. Therefore, the use of digital images from a smartphone as the future of cataract screening tools could be a practical and helpful solution for ophthalmologists, especially in rural areas with limited healthcare facilities.
Mapping Transformation Enabled High-Performance and Low-Energy Memristor-Based DNNs
Authors: Oli-Uz-Zaman Md. , Khan Saleh Ahmad , Yuan Geng , Liao Zhiheng , Fu Jingyan , Ding Caiwen , Wang Yanzhi , Wang Jinhui
Published: February 2022

When deep neural network (DNN) is extensively utilized for edge AI (Artificial Intelligence), for example, the Internet of things (IoT) and autonomous vehicles, it makes CMOS (Complementary Metal Oxide Semiconductor)-based conventional computers suffer from overly large computing loads. Memristor-based devices are emerging as an option to conduct computing in memory for DNNs to make them faster, much more energy efficient, and accurate. Despite having excellent properties, the memristor-based DNNs are yet to be commercially available because of Stuck-At-Fault (SAF) defects. A Mapping Transformation (MT) method is proposed in this paper to mitigate Stuck-at-Fault (SAF) defects from memristor-based DNNs. First, the weight distribution for the VGG8 model with the CIFAR10 dataset is presented and analyzed. Then, the MT method is used for recovering inference accuracies at 0.1% to 50% SAFs with two typical cases, SA1 (Stuck-At-One): SA0 (Stuck-At-Zero) = 5:1 and 1:5, respectively. The experiment results show that the MT method can recover DNNs to their original inference accuracies (90%) when the ratio of SAFs is smaller than 2.5%. Moreover, even when the SAF is in the extreme condition of 50%, it is still highly efficient to recover the inference accuracy to 80% and 21%. What is more, the MT method acts as a regulator to avoid energy and latency overhead generated by SAFs. Finally, the immunity of the MT Method against non-linearity is investigated, and we conclude that the MT method can benefit accuracy, energy, and latency even with high non-linearity LTP = 4 and LTD = −4.
Robust Path-Following Control of Underactuated AUVs with Multiple Uncertainties in the Vertical Plane
Authors: Miao Jianming , Deng Kankan , Zhang Wenrui , Gong Xi , Lyu Jifang , Ren Lei
Published: February 2022

The current study employs a novel nonlinear robust control approach for path-following control of underactuated autonomous underwater vehicles (AUVs) with multiple uncertainties in the vertical plane. Firstly, a nonlinear underactuated AUV model is established to characterize the dynamics of AUV and path-following error. To resolve dependence on a detailed model that appeared in previous studies, the unknown time-varying attack angular velocity in the dynamic model of the path-following error is considered as the kinematic uncertainty, while the linear superposition of the external environmental disturbances, the perturbations in the internal model parameters, and other unmodeled dynamics in the dynamic model is chosen as lumped dynamic uncertainties. Several reduced-order extended state observers (ESOs) are designed for estimating both of these uncertainties. Secondly, to reduce the impact of input saturation and avoid the “explosion of complexity” associated with traditional back-stepping method, a nonlinear track differentiator (NTD) is utilized to follow the virtual control signal and its derivative. Thirdly, the constructed reduced-order ESOs and NTD are adopted to establish an augmented back-stepping controller, where its ability to stabilize the overall system is demonstrated using the Lyapunov theorem. Finally, extensive simulations and analyses in various working conditions, including the nominal working condition without disturbances, the working condition with multiple uncertainties, and the conditions which better replicate the actual environment, are performed to demonstrate the effectiveness, superiority, and robustness of the designed controller.