bluuebunny/crossref_metadata_2025 huggingface dataset:
Bone marrow mesenchymal stem cells improve cognitive impairments induced by methamphetamine in rats and reduce relapse
Authors: Rafaiee Raheleh , Ahmadiankia Naghmeh , Mousavi Seyed Abbas , Jafari Behnaz , Kalalian Moghaddam Hamid
Published: February 2023

Introduction: Chronic exposure to methamphetamine (Meth) results in permanent central nervous system damage and learning and memory dysfunction. This study aimed at investigating the therapeutic effects of bone marrow mesenchymal stem cells (BMMSCs) on cognitive impairments in Meth addicted rats and comparing intravenous (IV) delivery with intranasal (IN) delivery of BMMSCs. Methods: Adult Wistar rats were randomly divided into 6 groups; Control; Meth-addicted; IV-BMMSC (Meth administered and received IV BMMSCs); IN-BMMSC (Meth administered and received IN BMMSCs); IV-PBS (Meth administered and received IV Phosphate-buffered saline (PBS); IN-PBS (Meth administered and received IN PBS). BMMSCs were isolated, expanded in vitro, immunophenotyped, labeled, and administered to BMMSCs-treated groups (2 × 106 cells). The therapeutic effect of BMMSCs was measured using Morris water maze and Shuttle Box. Moreover, relapse-reduction was evaluated by conditioning place preference after 2 weeks following BMMSCs administration. The expression of brain-derived neurotrophic factor (BDNF) and glial-derived neurotrophic factor (GDNF) in rat hippocampus was assessed using immunohistochemistry method. Results: Administration of BMMSCs caused a significant improvement in the learning and memory functions of Meth-addicted rats and reduced the relapse (P < 0.01). In behavioral tests, comparison of IV and IN BMMSC-treated groups did not show any significant difference. Administration of BMMSCs improved the protein level of BDNF and GDNF in the hippocampus, as well as causing behavioral improvement (P < 0.001). Conclusion: BMMSC administration might be a helpful and feasible method to treat Meth-induced brain injuries in rats and to reduce relapse. BMMSCs were significantly higher in IV-treated group compared to the IN route. Moreover, the expression of BDNF and GDNF was higher in IN-treated rats compared with IV treated group.
Mode of binding, kinetic and thermodynamic properties of a lipid-like drug (Fingolimod) interacting with Human Serum Albumin
Authors: Gholizadeh Samira , Haghaei Hossein , Karami Hosna , Soltani Somaieh , Zakariazadeh Mostafa , Shokri Javad
Published: February 2023

Introduction: Fingolimod is a drug that is used to treat multiple sclerosis (MS). It has pH-dependent solubility and low solubility when buffering agents are present. Multi-spectroscopic and molecular modeling methods were used to investigate the molecular mechanism of Fingolimod interaction with human serum albumin (HSA), and the resulting data were fitted to the appropriate models to investigate the molecular mechanism of interaction, binding constant, and thermodynamic properties. Methods: The interaction of Fingolimod with HSA was investigated in a NaCl aqueous solution (0.1 mM). The working solutions had a pH of 6.5. Data was collected using UV-vis, fluorescence quenching titrations, FTIR, and molecular modeling methods. Results: According to the results of the fluorescence quenching titrations, the quenching mechanism is static. The apparent binding constant value (KA = 4.26×103) showed that Fingolimod is a moderate HSA binder. The reduction of the KA at higher temperatures could be a result of protein unfolding. Hydrogen bonding and van der Waals interactions are the main contributors to Fingolimod-HSA complex formation. FTIR and CD characterizations suggested a slight decrease in the α-helix and β-sheets of the secondary structure of HSA due to Fingolimod binding. Fingolimod binds to the binding site II, while a smaller tendency to the binding site I was observed as well. The results of the site marker competitive experiment and the thermodynamic studies agreed with the results of the molecular docking. Conclusion: The pharmacokinetic properties of fingolimod can be influenced by its HSA binding. In addition, considering its mild interaction, site II binding drugs are likely to compete. The methodology described here may be used to investigate the molecular mechanism of HSA interaction with lipid-like drugs with low aqueous solubility or pH-dependent solubility.
Homozygous mutation in CSF1R causes brain abnormalities, neurodegeneration, and dysosteosclerosis (BANDDOS)
Authors: Daghagh Hossein , Rahbar Kafshboran Haniyeh , Daneshmandpour Yousef , Nasiri Aghdam Maryam , Talebian Shahrzad , Nouri Nojadeh Jafar , Hamzeiy Hamid , Biskup Saskia , Sakhinia Ebrahim
Published: February 2023

Introduction: The CSF1R gene encodes the receptor for colony-stimulating factor-1, the macrophage, and monocyte-specific growth factor. Mutations in this gene cause hereditary diffuse leukoencephalopathy with spheroids (HDLS) with autosomal dominant inheritance and BANDDOS (Brain Abnormalities, Neurodegeneration, and Dysosteosclerosis) with autosomal recessive inheritance. Methods: Targeted gene sequencing was performed on the genomic DNA samples of the deceased patient and a fetus along with ten healthy members of his family to identify the disease-causing mutation. Bioinformatics tools were used to study the mutation effect on protein function and structure. To predict the effect of the mutation on the protein, various bioinformatics tools were applied. Results: A novel homozygous variant was identified in the gene CSF1R, c.2498C>T; p.T833M in exon 19, in the index patient and the fetus. Furthermore, some family members were heterozygous for this variant, while they had not any symptoms of the disease. In silico analysis indicated this variant has a detrimental effect on CSF1R. It is conserved among humans and other similar species. The variant is located within the functionally essential PTK domain of the receptor. However, no structural damage was introduced by this substitution. Conclusion: In conclusion, regarding the inheritance pattern in the family and clinical manifestations in the index patient, we propose that the mentioned variant in the CSF1R gene may cause BANDDOS.
Differentiation of human endometrial stem cells encapsulated in alginate hydrogel into oocyte-like cells
Authors: Ghasemi Diba , Ebrahimi-Barough Somayeh , Nekoofar Mohammad Hossein , Mohamadnia Abdolreza , Lotfibakhshaiesh Nasrin , Bahrami Naghmeh , Karimi Roya , Taghdiri Nooshabadi Vajihe , Azami Mahmoud , Hasanzadeh Elham , Ai Jafar
Published: February 2023

Introduction: Human endometrial mesenchymal stem cells (hEnMSCs) are a rich source of mesenchymal stem cells (MSCs) with multi-lineage differentiation potential, making them an intriguing tool in regenerative medicine, particularly for the treatment of reproductive and infertility issues. The specific process of germline cell-derived stem cell differentiation remains unknown, the aim is to study novel ways to achieve an effective differentiation method that produces adequate and functioning human gamete cells. Methods: We adjusted the optimum retinoic acid (RA) concentration for enhancement of germ cell-derived hEnSCs generation in 2D cell culture after 7 days in this study. Subsequently, we developed a suitable oocyte-like cell induction media including RA and bone morphogenetic protein 4 (BMP4), and studied their effects on oocyte-like cell differentiation in 2D and 3D cell culture media utilizing cells encapsulated in alginate hydrogel. Results: Our results from microscopy analysis, real-time PCR, and immunofluorescence tests revealed that 10 µM RA concentration was the optimal dose for inducing germ-like cells after 7 days. We examined the alginate hydrogel structural characteristics and integrity by rheology analysis and SEM microscope. We also demonstrated encapsulated cell viability and adhesion in the manufactured hydrogel. We propose that in 3D cell cultures in alginate hydrogel, an induction medium containing 10 µM RA and 50 ng/mL BMP4 can enhance hEnSC differentiation into oocyte-like cells. Conclusion: The production of oocyte-like cells using 3D alginate hydrogel may be viable in vitro approach for replacing gonad tissues and cells.
Functionality of immune cells in COVID-19 infection: development of cell-based therapeutics
Authors: Sayahinouri Maryam , Mashayekhi Firouz Sahar , Ebrahimi Sadrabadi Amin , Masoudnia Mina , Abdolahi Mahnaz , Jafarzadeh Fatemeh , Nouripour Meshkat , Mirzazadeh Sana , Zangeneh Nazanin , Jalili Arsalan , Aghdami Nasser
Published: February 2023

Introduction: In late December 2019, a sudden severe respiratory illness of unknown origin was reported in China. In early January 2020, the cause of COVID-19 infection was announced a new coronavirus called severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Examination of the SARS-CoV-2 genome sequence revealed a close resemblance to the previously reported SARS-CoV and coronavirus Middle East respiratory syndrome (MERS-CoV). However, initial testing of drugs used against SARS-CoV and MERS-CoV has been ineffective in controlling SARS-CoV-2. One of the key strategies to fight the virus is to look at how the immune system works against the virus, which has led to a better understanding of the disease and the development of new therapies and vaccine designs. Methods: This review discussed the innate and acquired immune system responses and how immune cells function against the virus to shed light on the human body's defense strategies. Results: Although immune responses have been revealed critical to eradicating infections caused by coronaviruses, dysregulated immune responses can lead to immune pathologies thoroughly investigated. Also, the benefit of mesenchymal stem cells, NK cells, Treg cells, specific T cells, and platelet lysates have been submitted as promising solutions to prevent the effects of infection in patients with COVID-19. Conclusion: It has been concluded that none of the above has undoubtedly been approved for the treatment or prevention of COVID-19, but clinical trials are underway better to understand the efficacy and safety of these cellular therapies.
Modulating the tumor microenvironment improves antitumor effect of anti-PD-L1 mAb in breast cancer
Authors: Li Xiuying , Luo Xianqin , Hu Shunqin
Published: February 2023

Introduction: Immune checkpoint inhibitors (ICIs) have provided noteworthy benefits in multiple cancer patients. However, the efficacy of monotherapy of ICIs was very limited. In this study, we endeavored to explore whether losartan can modulate the solid tumor microenvironment (TME) and improve the therapeutic efficacy of anti-PD-L1 mAb in 4T1 mouse breast tumor model and the underlying mechanism. Methods: The tumor-bearing mice were treated with control agents, losartan, anti-PD-L1 mAb or the dual agents. The blood and tumor tissues were respectively used for ELISA and immunohistochemical analysis. CD8-depletion and lung metastatic experiments were performed. Results: Compared to control group, losartan inhibited the expression of alpha-smooth muscle actin (α-SMA), deposition of collagen I in the tumor tissues. The concentration of transforming growth factor-β1 (TGF-β1) in the serum was low in the losartan treated group. Although losartan alone was ineffective, the combination of losartan and anti-PD-L1 mAb elicited dramatic antitumor effect. Immunohistochemical analysis revealed that there were more intra-tumoral infiltration of CD8+ T cells and increased granzyme B production in the combination therapy group. In addition, the size of spleen was smaller in the combination therapy group, compared to monotherapy. The CD8-depleting Abs abrogated the antitumor efficacy of losartan and anti-PD-L1 mAb in vivo. The combination of losartan and anti-PD-L1 mAb significantly inhibited 4T1 tumor cells lung metastatic in vivo. Conclusion: Our results indicated that losartan can modulate the tumor microenvironment, and improve the efficacy of anti-PD-L1 mAb.
Platinum(IV) compounds as potential drugs: a quantitative structure-activity relationship study
Authors: Novak Jurica , Zykova Alena R. , Potemkin Vladimir A. , Sharutin Vladimir V. , Sharutina Olga K.
Published: February 2023

Introduction: Machine learning methods, coupled with a tremendous increase in computer power in recent years, are promising tools in modern drug design and drug repurposing. Methods: Machine learning predictive models, publicly available at chemosophia.com, were used to predict the bioactivity of recently synthesized platinum(IV) complexes against different kinds of diseases and medical conditions. Two novel QSAR models based on the BiS algorithm are developed and validated, capable to predict activities against the SARS-CoV virus and its RNA dependent RNA polymerase. Results: The internal predictive power of the QSAR models was tested by 10-fold cross-validation, giving cross-R2 from 0.863 to 0.903. 38 different activities, ranging from antioxidant, antibacterial, and antiviral activities, to potential anti-inflammatory, anti-arrhythmic and anti-malarial activity were predicted for a series of eighteen platinum(IV) complexes. Conclusion: Complexes 1, 3 and 13 have high generalized optimality criteria and are predicted as potential SARS-CoV RNA dependent RNA polymerase inhibitors.
Fabrication of mesoporous silica nanoparticles for targeted delivery of sunitinib to ovarian cancer cells
Authors: Torabi Mitra , Aghanejad Ayuob , Savadi Pouria , Barzegari Abolfazl , Omidi Yadollah , Barar Jaleh
Published: February 2023

Introduction: Mesoporous silica nanoparticles (MSNPs) are considered innovative multifunctional structures for targeted drug delivery owing to their outstanding physicochemical characteristics. Methods: MSNPs were fabricated using the sol-gel method, and polyethylene glycol-600 (PEG600) was used for MSNPs modification. Subsequently, sunitinib (SUN) was loaded into the MSNPs, MSNP-PEG and MSNP-PEG/SUN were grafted with mucin 16 (MUC16) aptamers. The nanosystems (NSs) were characterized using FT-IR, TEM, SEM, DLS, XRD, BJH, and BET. Furthermore, the biological impacts of MSNPs were evaluated on the ovarian cancer cells by MTT assay and flow cytometry analysis. Results: The results revealed that the MSNPs have a spherical shape with an average dimension, pore size, and surface area of 56.10 nm, 2.488 nm, and 148.08 m2g-1, respectively. The cell viability results showed higher toxicity of targeted MSNPs in MUC16 overexpressing OVCAR-3 cells as compared to the SK-OV-3 cells; that was further confirmed by the cellular uptake results. The cell cycle analysis exhibited that the induction of sub-G1 phase arrest mostly occurred in MSNP-PEG/SUN-MUC16 treated OVCAR-3 cells and MSNP-PEG/SUN treated SK-OV-3 cells. DAPI staining showed apoptosis induction upon exposure to targeted MSNP in MUC16 positive OVCAR-3 cells. Conclusion: According to our results, the engineered NSs could be considered an effective multifunctional targeted drug delivery platform for the mucin 16 overexpressing cells.
Utilization of dental care services among adult Indian population: A meta-analysis of evidence from 2011–2022
Authors: Talukdar Rounik , Barman Diplina , Thakkar Vallabh , Kanungo Suman
Published: February 2023

Background: This study aimed to generate a pooled national estimate on dental health care services utilization by the adult population in India from any public or private facility in an effort to highlight the demand and usage for oral health care. Methods: In this meta-analysis, PubMed, ScienceDirect, DOAJ, and Google Scholar were searched using a search strategy that combined MeSH headings and keywords (e.g., "Oral Health", "Dental Health Services", utilization, India, etc.) for articles on dental utilization among Indian adults, published between January 2011 and June 2022. Study quality was assessed using the NIH Quality assessment tool, and a random-effects inverse-variance method was used for pulling utilization proportions. Meta-regression and sub-group analyses were conducted to identify the sources of heterogeneity. Heterogeneity is reported as I2 . To examine publication bias, the funnel plot, egger’s test, and trim-and-fill analysis were performed. Results: From 4012 identified articles, 21 were eligible for inclusion. The pooled dental care utilization amongst Indian adults were found to be 23.96% (confidence interval [CI]: 16.81%– 31.11%, P<0.001, I2=98.93%), and the highest estimate was in South Zone (30.02%, CI: 19.14–40.90, P<0.01, I2=98.63%). Visual inspection of the funnel plot revealed the presence of publication bias (egger’s P value 0.02). A mild decrease in utilization estimate was noted through trim and fill analysis (adjusted estimate 17.65%, CI: 8.97–26.33, P=0.03). No significant subgroup effect was found for the variables study zone and conduction years (P value: 0.09 & 0.34 respectively). Conclusion: Future studies should be undertaken to focus on the demand and supply of oral health care services since an evidential gap has been identified due to the uneven distribution of studies available from various regions of India. The heterogeneity can be attributed to the diverse socioeconomic, literacy, and inherent health system performance status.
Years of life lost: A call to achieve equitable end-of-life care among children
Authors: Jawed Aysha
Published: February 2023

Significant disparities continue to exist in access to inpatient pediatric hospice care among children at the end-of-life. Increasingly more children at this stage are dying in the hospital or at home on hospice which is not always an acceptable option to the children and their families. Two clinical case examples illustrate implementation of these options in practice. A missing link exists in healthcare systems across developed and developing countries in pediatric end-of-life care. Currently, the primary options involve selecting between hospital and home-based hospice care. Proposing to increase access to inpatient pediatric hospice services could potentially increase acceptability of this option to honor the child in line with the family’s preferences, goals, wishes, and values. In addition, inpatient pediatric hospice could offset costs from preventable hospitalizations and overall high-cost healthcare utilization. Oftentimes, readmissions impact decision-making among caregivers that include changes in code status from Do Not Resuscitate/Do Not Intubate (DNR/DNI) to full curative care, thereby resulting in medicalization or overmedicalization of the child. It follows that reduced healthcare expenditures will increase cost efficiency across the healthcare system. Achieving health equity in palliative care among adult and pediatric patients at the end-of-life is a longstanding goal of the World Health Organization (WHO) and the United Nations International Children’s Emergency Fund (UNICEF). Proposing to mitigate disparities in palliative care among children through inpatient hospice as another viable option for their families could contribute to the larger overarching goal of achieving health equity in end-of-life care across the world.
COVID-19 vaccination hesitancy for children: A pilot assessment of parents in the United States
Authors: Batra Kavita , Sharma Manoj , Dai Chia-Liang , Batra Ravi , Khubchandani Jagdish
Published: February 2023

Background: Coronavirus disease 2019 (COVID-19) vaccine hesitancy has remained a significant concern among adults worldwide. However, not much is known about parental vaccine hesitancy for getting children vaccinated for COVID-19 in the U.S. Thus, the purpose of this study was to conduct a national assessment of parents’ preferences for COVID-19 vaccination of children using the evidence-based Multi-Theory Model (MTM) and explore the predictors of vaccine hesitancy. Methods: To participate in this study, a national random sample of parents (n=263) took a valid and reliable online questionnaire based on the MTM. Independent samples t test, chi-square test, multiple logistic regression was utilized to analyze data. Results: More than two-fifths (42%) of the participating parents were not willing to get their children vaccinated for COVID-19. Parental vaccination status, booster dose acceptance, education, and political affiliation were significant predictors of willingness to get children vaccinated for COVID-19. In the multiple logistic regression analyses, behavioral confidence and participatory dialogue (i.e., perceived advantages versus disadvantages) were statistically significant predictors of COVID-19 vaccination hesitancy for children among the participating parents. Conclusion: Given the multiple factors that were found influential in parental hesitancy for COVID-19 vaccination among children, multimodal and evidence-based interventions are needed to increase the uptake of COVID-19 vaccines among children by influencing the parents’ perceptions, increasing their confidence, dispelling misinformation, and reducing constraints for vaccination. Such interventions should emphasize communication and messaging that is truthful, interactive, scientifically correct, and to be delivered in a variety of community-based settings.
Color stability of aligner materials on exposure to indigenous food products: An in-vitro study
Authors: Venkatasubramanian Priyanka , Jerome Mary Sanjana , Ragunanthanan Lokamithra , Maheshwari Uma , Vijayalakshmi Devaki
Published: February 2023

Background. In the recent day, there has been an exponential growth in the usage of clear aligners for orthodontic treatment. As with any removable appliance, the compliance of patients to remove it during ingestion of food is, at times, poor. Thus, the stability of the clear aligner to be "clear" becomes questionable. This in-vitro study examined how the clear aligners changed colour on exposure to various indigenous food products used in everyday life. Methods. Aligners from 5 different companies (K Line, Clearbite Aligners, The Aligner Company, iAligners and MaxDent CA Digital) were exposed for 12 hours and 24 hours to various indigenous substances (tea, green tea, coffee, turmeric, saffron and Kashmiri red chili powder) and a control solution (distilled water) in-vitro. The color change was assessed with the help of VITA Easyshade compact colorimeter based on Commission Internationale de I’Eclairage L*a*b* color system. Values were then modified to NBS units for clinical relevance. Results. The hue of the transparent aligners was noticed to change in a statistically meaningful way when exposed to turmeric, saffron, Kashmiri red chili powder and coffee in decreasing order and mild color change in tea and green tea at both 12 hours and 24 hours intervals. Conclusion. Aligners are prone to color change when exposed to indigenous foods that contain staining properties.
Effect of using calcium-silicate and silicone based root canal sealers in bulk or with main core material on bond strength
Authors: Kadı Gizem , Özlek Esin , Saed Yousef
Published: February 2023

Background. The purpose of this study was to assess the push-out bond strength of calcium-silicate and silicone based root canal sealers in bulk and with main cone. Methods. Roots (n=48) randomly divided into 4 groups (n=12) according to the obturation protocol; (1) iRoot SP in bulk; (2) iRoot SP with gutta-percha; (3) GuttaFlow Bioseal in bulk; (4) GuttaFlow Bioseal with gutta-percha. Six horizontal sections were obtained from each root (n=72). Effect of sealers on bond strength was statistically significant (P<0.05). Results. Highest mean value was obtained in iRoot-Bulk group and lowest in GuttaFlow Bioseal-GP group. Both iRoot SP groups had significantly higher bond strength values than both GuttaFlow Bioseal groups (P<0.05). There was no significant difference between iRoot-GP and iRoot-Bulk groups (P=0.603) also GuttaFlow Bioseal-GP and GuttaFlow Bioseal-Bulk groups (P=0.684). Conclusion. Based on findings, using calcium silicate-based sealer in bulk can be also suitable in clinical practice.
Large dentigerous cyst associated with the maxillary impacted supernumerary teeth: A rare occurrence and literature review
Authors: Aoki Noriaki , Matumoto Megumi , Ishii Soichiro , Okuma Yasuaki , Umezawa Himiko , Baba Junichi , Ito Takaaki
Published: February 2023

Dentigerous cysts are common odontogenic cysts of the jaw but are rarely associated with supernumerary teeth. Few cases of large dentigerous cysts associated with anterior maxillary supernumerary teeth have been reported. The English literature has documented only four cases of dentigerous cysts>40 mm in diameter associated with supernumerary teeth. A 47-year-old man was referred to our hospital, complaining of minor pain in the maxillary gingiva. Computed tomography revealed a well-defined oval unilocular radiolucent lesion (50×45×35 mm) in the right maxilla, including two impacted supernumerary teeth. A dentigerous cyst associated with impacted anterior maxillary supernumerary teeth was diagnosed. The two impacted teeth were surgically removed, and the cyst was enucleated using the Caldwell-Luc approach. Histopathology confirmed the diagnosis of a large dentigerous cyst associated with impacted anterior maxillary supernumerary teeth. The postoperative course has been uneventful for two years. We also reviewed the relevant English literature.
Emotional drill as a way to develop the ability to accept oneself and others without judgement
Authors: Saveleva Olga E.
Published: February 2023

The modern school with its nurturing function has to deal with numerous factors that negatively affect interpersonal relations between young people. Therefore that appears to be of great importance and timely to reinforce the Education Standards (ES) of the Russian Federation with a provision that declares the need to teach schoolchildren “to accept themselves and others without judgement”. However, while analysing the publishing statistics of the Russian Internet library elibrary.ru, the author reveals that realisation of this provision is far from being sufficiently supported with research and methodological literature. On the other hand, foreign countries (with the example of the USA) have long been thoroughly researching and designing practical programmes for social and emotional learning (SEL). This work is largely close to the process of fostering the ability to accept oneself and others without judgement, since such programmes include the development of empathy, understanding and managing emotions, as well as teaching conflict resolution. The article highlights the great potential of the SEL programmes and suitability of applying them in the Russian system of education. Besides, based on the behaviouristic approach, the author proposes original techniques of emotional drill centred around anchor images, which could be used for putting into practice the analysed provision of the Russian ES. The article is exploratory and disputable with the results able to be used in further research of ways to increase the nurturing potential of schools, ways to foster emotional intelligence and empathy, to develop spiritual and morale traits of schoolchildren and the culture of conflict-free interaction.
Specifics of teaching grammar aspect of second foreign language
Authors: Babenko Marina G. , Khramova Tatiana Yu.
Published: February 2023

This article examines the problem of using effective methods of teaching the grammatical aspect of a second foreign language. It highlights the importance of grammatical skills and abilities for the successful formation of foreign language communicative competence. It is revealed that the methodology of teaching grammar should be based on a rational combination of comparative and communicative-cognitive approaches. The authors lay emphasis on the necessity of relying on the first foreign languageand the linguistic competence formed during its study. In addition, the results of a survey of students are presented, which made it possible to identify the main grammatical difficulties and cases of interference. The analysis of students' monologues and written works made it possible to determine the most frequent mistakes that distort the meaning and hinder communication. Based on the results of an empirical study and data obtained, productive methods of teaching the second foreign language grammar are presented.
About modelling the process of formation of vocal and performing culture of younger schoolchildren in Music lessons
Authors: Kashapova Lyalya M. , Ni Zhen
Published: February 2023

The aim of the study was to model the pedagogic process that determines the optimisation of the formation of vocal and performing culture of younger schoolchildren in a music lesson at school. The article presents a structural and functional model that determines the context of reading musical culture by means of singing. The scientific novelty of the research consists in the development of the content that determines the success of the transformative impact of singing culture on the initiation of the need for creativity in a child at the stage of primary general education. The model, in turn, provides the logic of the chosen actions by designating the principles as the dominant ideas of moving towards the goal – integrity, dialogicity, variability, accessibility. During the generalisation of the experimental work, the key parameters of the results of the developed design in the educational reality of primary school were the proportional ratio between the components, criteria and indicators of the formation of vocal and performing culture among pupils as a syncretic part of the general culture.
Use of Blockchain to Improve Case Studies in Food Supply
Authors: Haryanto  , Fiananda Rio Argi , Shilvia Wanri  , Sunarsih
Published: February 2023

The current food supply is a linear economic model that directly or indirectly fulfills needs. However, this model has several weaknesses, such as the relationship between members of the supply chain or the lack of information to consumers about the origin of the product. In this paper we propose a new supply chain model via blockchain. This new model enables the concept of a circular economy and removes many of the disadvantages of the current supply chain. To coordinate all the transactions that occur in the food supply, a multi-agent system is created for this paper.
Topical Issues of Warfarin Therapy Control
Authors: Серебро А.М.
Published: February 2023

Антагонисты витамина К (АВК) широко назначаются во всем мире. Определяющим в вопросе эффективности терапии антагонистами витамина К является целевой диапазон международного нормализованного отношения (МНО), стараться достичь которого необходимо у каждого пациента. У пациентов, получающих терапию АВК, требуется мониторинг протромбинового времени (ПВ) в единицах МНО для оценки эффективности антикоагулянтной терапии. Серьезной проблемой, связанной с терапией варфарином, является непредсказуемость выраженности терапевтического эффекта при стандартном режиме дозирования. Использование портативных коагулометров (портативных анализаторов свертываемости крови) для наблюдения за пациентами, получающими лечение АВК, позволяет немедленно получить результаты международного нормализованного отношения и легко контролировать эффективность терапии у пациентов, находящихся на амбулаторном лечении.

Vitamin K antagonists (VCA) are widely prescribed worldwide. The target range of INR, which is necessary for each patient, is decisive in the issue of the effectiveness of vitamin K antagonists’ therapy. In patients receiving AVC therapy, prothrombin time (PV) monitoring in INR (International Normalized Ratio) units is required to assess the effectiveness of anticoagulant therapy. A serious problem associated with warfarin therapy is the unpredictability of the severity of the therapeutic effect at the standard dosing regimen. The use of portable coagulometers to follow-up patients receiving vitamin K antagonists (AVC) treatment allows immediate results of the international normalized ratio (INR) and an easy monitoring of therapy effectiveness in outpatients.
CATALASE ACTIVITY IN INTERACTION WITH IONIZED WATER AND OTHER ANTIOXIDANTS IN BLOOD PLASMA, LIVER, AND KIDNEY OF THE RAT DURING HYPERTHERMIC STRESS
Authors: Ademi Majlinda
Published: February 2023

The similarity between the responses seen after heat stress and those happening in the condition of oxidative stress suggests that heat stress is an environmental element that stimulates the generation of reactive oxygen species (ROS). Alkaline water, also known as ionized or reduced water (ERW), is water that has undergone electrochemical activation and has a pH greater than 7. The ERW also has excellent redox properties and other reducing features. ERW mimics the activity of the antioxidant enzyme, such as catalase (CAT) by scavenging ROS. The aim of this study was to examine the catalase activity in interaction with ERW under hyperthermic stress by including non-enzymatic antioxidants, glutathione, and vitamin C. White laboratory Young female Wistar rats weighing 180–220 g were divided into three groups of 15 for the experiment. Oxidative stress was caused by 41°C acute hyperthermic exposure. The first group is referred to as the control group (CPM), the second group is referred to as the ionized water treatment (TAM), and the third group is referred to as the ionized water treatment with added glutathione and vitamin C (TAD). The treatment period lasted 21 days. The treatment applied respectively to each group during the period of hyperthermic exposure caused a significant difference in CAT activity in blood plasma among the three groups. Liver CAT activity was increased in all three groups. Treatment for 21 days in all three groups led to a decrease in CAT activity in the blood plasma and in the kidneys. Acute hyperthermic exposure on the 21st day in the CPM and TAD groups for blood plasma has a statistically significant difference (p < 0.01). Also, in both the TAM and TAD groups there is a statistically significant difference (p < 0.01) in CAT activity, which is in contrast to the difference in the liver and kidney CAT activity between the remaining compared groups, which was shown to be statistically insignificant.
PENGARUH PENGGUNAAN MODEL PEMBELAJARAN LEARN ON DELIVERY (LEADER) TERHADAP HASIL BELAJAR SISWA PADA PEMBELAJARAN BIOLOGI KELAS X DI SMA MUHAMMADIYAH BOARDING SCHOOL TARAKAN
Authors: Ramadani Bhekti , Wijarini Fitri , Adhani Aidil
Published: February 2023

Abstract : This research aimed to figure out the influence of Learn On Delivery (Leader) learning model on biology learning outcomes of Grade X students at SMA Muhammadiyah Boarding School Tarakan. The research employing Quasi Experimental method with a Non Equivalent Control Group Design was conducted in March for two meetings with the samples of X mia 1 (experimental class) and X mia 2 (control class). The data were collected using observations, formative tests in the form of 30 multiple choice questions, and documentation. The normality test shows tha significance value was 0.05, meaning that the samples were normally distributed. The homogeneity tests shows the value of 0.185 and 0.140 (sig 0.05), meaning that the data is homogeneous. The research results based on the Analysis of Covariance (anacova) show that Learning On Delivery (Leader) learning model which influenced learning outcomes was feasible seen from the sig value of 0.000 ( 0.05) with F value of 46.981 (corrected model) in the table (tests of between-subjects effects) with the significant value of 0.000 (p0.05), meaning that H0 was rejected. The role of “covariates” is determining the class average differences could be seen from pretest scores in which the significance value was 0.166 ( 0.05). Thus, in this anacova model, the role of covariate in pretest was not significant. Keywords : learning model, Learn On Delivery (Leader), Learning outcomes
PERSEPSI SISWA TERHADAP MEDIA PEMBELAJARAN DALAM JARINGAN (Daring) PADA MATA PELAJARAN BIOLOGI DI MADRASAH ALIYAH NEGERI TARAKAN
Authors: Nurlina Nurlina , Wijarini Fitri , Adhani Aidil
Published: February 2023

Abstract: This study aims to look at students' perceptions of online learning media in Biology subjects at MAN Tarakan. This type of research is a survey research with a qualitative descriptive method. The population in this study was 200 students with a total sample of 66 students. Overall data was obtained based on scores and answers from student perception questionnaires distributed online via Google form. The results showed that students' perceptions of the use of learning media in the network system (Online) in Biology subjects at MAN Tarakan students had good perceptions of the platform as learning media in the network system (Online) in Biology subjects. Keywords: Perception, Media, Online.
ANALISIS TREN PENELITIAN PENDIDIKAN BIOLOGI SELAMA 1 DEKADE (2011-2020) DI UNIVERSITAS BORNEO TARAKAN
Authors: Damanik Lolyta , Abrori Fadhlan Muchlas , Adhani Aidil
Published: February 2023

This study aimed to analyze trends in Biology Education research for a decade (2011-2020) at the University of Borneo Tarakan. This literature study research used a quantitative approach. The subject in this study was the undergraduate theses of biology education students in 2011-2020 who submitted to the UBT library. The trend was seen from the dimensions of the approach used, the research method, the field of research, and the research sample. The collected theses were based on the data collected from the library, namely 315 theses. The results showed that the quantitative approach had a significant value compared to other approaches with a percentage value of 53%, followed by a combination approach of 37% and a qualitative approach of 10%. Furthermore, based on the fields used, it was found that 84% were in the field of biology education, and 16% were in pure biology. Meanwhile, when viewed from the type/research method, the quasi-experiment and RD with samples were widely used by biology education students at the junior and senior high school levels. Keywords: research trend, approach, type/method, sample
Hubungan Antara Keterampilan Proses Sains Terintegrasi dan Efikasi Diri dengan Literasi Biologi Siswa
Authors: Wulaningsih Ratna Dewi , Sartono Nurmasari , Nurmanita Sarah Rahmawati
Published: February 2023

The rapid development of science and technology in the era of globalization requires educational institutions to be able to form students who have scientific literacy skills. Biology is a branch of science. Biological literacy is the development of scientific literacy in a biological context. Science problems, especially biology, that exist in everyday life can be solved if students understand and can use integrated science process skills and high self-efficacy. It is intended that every decision taken when solving problems has a strong foundation and students will be able to develop in achieving achievements and feel optimistic in achieving something. This study aims to determine the relationship between integrated science process skills and self-efficacy with students' biological literacy. This research was conducted at SMA Negeri 3 Depok in the odd semester 2021/2022 academic year. The method used is descriptive method, with the type of correlational study. The sample used was 144 students taken from four classes of X Mathematics and Natural Sciences using simple random sampling technique. The data in this study were taken using three test instruments, namely: a biological literacy test, an integrated science process skills test and a self-efficacy test. The results showed that there was a positive relationship between integrated science process skills and biological literacy, a positive relationship between self-efficacy and biological literacy, a positive relationship between integrated science process skills and students' self-efficacy and biological literacy. Keywords: self-Efficacy, integrated science process skills, biological literacy
Analisis Kemampuan Berpikir Kritis Peserta Didik Melalui Implementasi Pembelajaran Online
Authors: Sonya Sonya , Ibrahim Ibrahim , Fatmawati Fatmawati
Published: February 2023

This study aimed to determine the critical thinking skills of students at MTs Negeri Tarakan through the implementation of online learning. This pre-experimental research used one shot case study design. The research subjects were students of class VIII C of MTs Negeri Tarakan, where sample was determined using a purposive sampling technique. The data was obtained from the results of critical thinking ability test in the form of description questions. The results of the one sample t-test showed that the t-count = -17.106 with dk= 34 and the t-table = 1.691. As the t-count t-table, the Ho was accepted, meaning that the average value of students’ critical thinking skills was lower than 70. The percentage of achievement of each indicator or critical thingking ability were 52% for providing a simple explanation (modearate category), 39% for concluding indicators (low category), 56% for providing further explanation (moderate category), and 71% for setting strategies and tactics (high category). The average achievement of critical thingking ability indicators was 54% (moderate category). Thus, it was necessary to train students’ critical thingking skills through the learning process by using the right model and applying critical thinking indicators. Keywords:  Critical Thinking Ability, Online Learning, Indicator
PENGEMBANGAN MEDIA PEMBELAJARAN POP-UPSNAKES AND LADDERS PADA MATERI ARCHAEBACTERIA DAN EUBACTERIA KELAS X SMA/MA DI DAERAH PERBATASAN
Authors: Novianti Nilam , Nugroho Endik Deni , Ilma Silfia
Published: February 2023

Penelitian ini bertujuan untuk menganalisis kelayakan media pembelajaran Pop-up Snakes and Ladders pada materi Archaebacteria dan Eubacteria untuk kelas X SMA/MA di daerah perbatasan berdasarkan ahli materi, ahli media, ahli praktisi dan respon siswa. Penelitian  yang  digunakan  adalah  penelitian  pengembangan  atau Research and Development (RD) yang mengacu pada model 4-D (four D)  yang dikembangkan oleh Thiagarajan, dkk. 1974. Model ini meliputi tahap pendefinisian (define), perancangan (design), pengembangan (develop) dan penyebaran (disseminate). Namun dalam penelitian ini, peneliti mengembangkan sampai tahap D yang ketiga, yaitu tahap develop dikarenakan keterbatasan waktu dan biaya.Hasil analisis validasi ahli materi diperoleh presentase sebesar 93,25% dengan kategori sangat layak. Analisis ahli media diperoleh presentase sebesar 83% dengan kategori sangat layak. Sedangkan ahli praktisi diperoleh presentase sebesar 88% dengan kategori sangat layak. Dari hasil analisis data secara keseluruhan, maka dapat disimpulkan media yang dikembangkan telah memenuhi standar kelayakan media dengan tingkat kelayakan yaitu sangat layak sebagai media pembelajaran.
KAJIAN ETNOBOTANI TUMBUHAN OBAT SUKU DAYAK LUNDAYEH DI DESA KALIAMOK KECAMATAN MALINAU UTARA KABUPATEN MALINAU SEBAGAI BOOKLET UNTUK MASYARAKAT
Authors: Setiawan Ade , Listiani Listiani , Abrori Fadhlan Muchlas
Published: February 2023

Penelitian ini bertujuan untuk mengetahui jenis-jenis tumbuhan obat yang digunakan oleh suku Dayak Lundayeh dan untuk mengetahui kualitas booklet tumbuhan obat dilihat dari segi kelayakan. Jenis penelitian ini adalah penelitian dan pengembangan (Research and Development) dengan model pengembangan Borg Gall (1983) yang dilakukan sampai tahap ke 7. Hasil penelitian menunjukkan bahwa terdapat 21 tumbuhan yang digunakan oleh suku Dayak Lundayeh sebagai obat dan tumbuhan tersebut terbagi dalam 13 famili. Dari 13 famili yang digunakan sebagai obat, tumbuhan dari famili Asteraceae dan family Zingiberaceae yang paling banyak digunakan. Hasil dari identifikasi jenis tumbuhan obat, kemudian dikembangkan menjadi booklet. Selanjutnya dilakukan validasi terhadap booklet oleh ahli materi, ahli media dan ahli bahasa. Hasil penilaian ahli materi mendapatkan persentase kelayakan 93,3% dengan kriteria sangat layak, ahli media mendapatkan persentase 85% dengan kriteria layak, dan ahli bahasa mendapatkan persentase 93,3% dengan kriteria sangat layak. Pada uji lapangan awal mendapatkan nilai persentase kelayakan 98,8% dengan kriteria sangat layak sedangkan pada uji lapangan utama mendapatkan nilai persentase kelayakan sebesar 99,55% dengan kriteria sangat layak.
ANATOMI TUMBUHAN OBAT: CIPLUKAN (Physalis angulata)
Authors: Vlorensius Vlorensius
Published: February 2023

Pyhsalis angulata merupakan family Solanaceae. Masyarakat umum mengetahui tumbuhan tersebut sebagai obat. Tumbuhan sebagai obat umumnya memproduksi senyawa obat yang disimpan dalam struktur khusus, sehingga penelitian ini bertujuan untuk mengamati anatomi P. angulata untuk menemukan struktur khusus yang diduga menyimpan senyawa obat. Pengamatan  anatomi menggunakan mikroskop dan pewarnaan safranin. Berdasarkan pengamatan, jaringan yang dijumpai yaitu jaringan yang paling umum seperti jaringan epidermis, jaringan dasar, jaringan penguat (Kolenkim dan sklerenkim) dan jaringan pengangkut. Struktur khusus berupa trikoma dijumpai di daun. Struktur tersebut diduga memproduksi senyawa sebagai bahan obat.
KAJIAN BIOLOGI DAN BUDAYA MASYARAKAT SUKU DAYAK DAN TIDUNG DI DAERAH TARAKAN DALAM PEMANFAATAN BUAH-BUAHAN
Authors: Indriani Indriani , Anggraini Bella , Halimah Putri
Published: February 2023

Tujuan penelitian ini adalah untuk mengidentifikasi jenis buah-buahan yang digunakan dalam kehidupan sehari-hari Suku Tidung dan Dayak di Tarakan. Pengumpulan data menggunakan teknik snowball sampling, dan total orang yang diwawancarai sebanyak 6 orang. Hasil dari penelitian didapatkan terdapat 25 jenis buah-buahan yang digunakan olah Suku Tidung dan dayak, dan Genus Artocarpus merupakan jenis yang terbanyak dibandingkan yang lainnya.
PENGEMBANGAN MEDIA PEMBELAJARAN BIOLOGY SMART APPS (BISA) PADA MATERI ARCHAEBACTERIA DAN EUBA CTERIA UNTUK SISWA KELAS X SMA/MA
Authors: Dahlia Dahlia , Nugroho Endik Deni , Abrori Fadhlan Muchlas
Published: February 2023

Tujuan penelitian ini adalah untuk mengetahui kualitas media pembelajaran Biology Smart Apps (BISA) berdasarkan penilaian ahli dan respon siswa pada materi Archaebacteria dan Eubacteria.  Pengembangan media pembelajaran Biology Smart Apps (BiSA) mengadaptasi alur pengembangan model Luther yang terdiri dari 5 tahapan yaitu tahap concept (konsep), design (perancangan), material collecting (pengumpulan bahan), assembly (pembuatan), dan testing (pengujian).  Hasil penelitian berdasarkan penilaian ahli dan praktisi mendapatkan kriteria sangat valid, dengan rincian penilaian dari ahli media sebesar 85% (sangat valid), ahli materi I sebesar 88% (sangat valid), ahli materi II sebesar 85% (sangat valid) dan praktisi sebesar 91% (sangat valid). Selanjutnya, Media pembelajaran Biology Smart Apps (BiSA) pada materi Archaebacteria dan Eubacteria untuk siswa kelas X SMA/MA berdasarkan uji lapangan mendapatkan kriteria sangat layak digunakan sebagai media pembelajaran dengan perolehan persentase respon siswa sebesar 83% .Kata kunci : Biology Smart Apps, Archaebacteria, Eubacteria