bluuebunny/crossref_metadata_2025 huggingface dataset:
Vad gör socionomerna för skillnad i den psykiatriska vården?
Authors: Framme Gunilla
Published: October 2020

I de flesta vuxenpsykiatriska team ingår en eller flera socionomer, vars psykosociala syn- och arbetssätt ses som ett väsentligt inslag i en huvudsakligen medicinsk kontext. De senaste årens utveckling på psykiatriområdet har ställt dem inför nya utmaningar med ökade krav på att tydliggöra sin yrkesroll och legitimera sin existens inom vården.I denna artikel diskuteras hur ett antal socionomer beskriver och värderar sitt specifika bidrag i det professionella teamarbetet.
Valfrihet istället för jämlikhet – förändrade målsättningar för äldreomsorgspolitiken?
Authors: Katzin Mirjam
Published: October 2020

Utvecklingen av valfrihetssystem i äldreomsorgen innebär ytterligare ett steg i förändringen mot en marknadsorienterad välfärd. Denna artikel diskuterar, genom teorier om det sociala medborgarskapet, huruvida socialtjänstlagens (2001:453) portalparagraf (1 kap. 1 §), vilken anger att socialtjänsten ska främja jämlikhet i levnadsvillkor, kan komma i konflikt med valfrihetssystem i äldreomsorgen.
Lägst ned på skalan? Hälso- och sjukvårdens bemötande av äldre kvinnor som migrerat till Sverige
Authors: Brodin Helene , Matisson Titti
Published: October 2020

Det professionella bemötandet inom vård och omsorg kan påverkas av omedvetna värderingar som gör att personer upplever sig kränkta eller särbehandlade. Den här artikeln utforskar hur hälso- och sjukvården bemöter äldre kvinnor som migrerat till Sverige. Vi diskuterar spänningen mellan lika och likvärdigt bemötande och vilken relevans detta har för professionsutbildningar inom vård och omsorg.
Haptic Glove Using Tendon-Driven Soft Robotic Mechanism
Authors: Baik Siyeon , Park Shinsuk , Park Jaeyoung
Published: October 2020

Recent advancements in virtual reality and augmented reality call for light-weight and compliant haptic interfaces to maximize the task-performance interactivity with the virtual or extended environment. Noting this, we propose a haptic glove using a tendon-driven compliant robotic mechanism. Our proposed interface can provide haptic feedback to two fingers of a user, an index finger and a thumb. It can provide both cutaneous and kinesthetic feedback to the fingers by using the tendon-driven system. Each actuator is paired with a force sensor to exert the desired tension accurately. In order to optimize the perception of the kinesthetic feedback, we propose a perception-based kinesthetic feedback distribution strategy. We experimentally measured the force perception weight for peripheral interphalangeal (PIP) and metacarpophalangeal (MCP) joints. We observed no significant difference in the force perception between the two joints. Then, based on the obtained weights, our proposed force distribution method calculates the force for each joint. We also evaluated the effect of additional cutaneous feedback to the kinesthetic feedback, on the force perception at the fingertip. The experimental result has shown that additional cutaneous feedback has significantly increased the sensitivity of the human perception. Finally, we evaluated our proposed system and force distribution algorithm by conducting a human subject test. The experimental result indicates that the availability of the cutaneous feedback significantly improved the perceived realism and acuity of the contact force.
Social Acceptance in Inclusive Classrooms: The Role of Teacher Attitudes Toward Inclusion and Classroom Management
Authors: Garrote Ariana , Felder Franziska , Krähenmann Helena , Schnepel Susanne , Sermier Dessemontet Rachel , Moser Opitz Elisabeth
Published: October 2020

Social acceptance is vital to students’ development. Being rejected by classmates can result in negative socio-emotional and academic outcomes. Finding relevant factors to be able to effectively support student social acceptance is especially challenging in inclusive classrooms because of the high heterogeneity of the student group. There is evidence that social acceptance is determined by the social behavior of students. In addition, current research suggests that affect-motivation dispositions, such as teacher attitudes, are related to teaching practices, which in turn are associated with student outcomes. This longitudinal study examines, on an individual level, the relationship between social behavior and the social acceptance of students. On a classroom level, the extent to which a teacher’s attitudes toward the inclusion of students with special educational needs affects their classroom management (i.e., implementation of clear rules and successful time management) is analyzed. In addition, the effect of teacher attitudes toward inclusion and classroom management on social acceptance in the classroom is investigated. The social acceptance of a sample of 580 students in 34 inclusive classrooms was assessed at the beginning and the end of the school year. In addition, student social behavior was rated by peers at the beginning of the school year. Teachers (n = 34) were asked about their attitudes toward inclusion at the beginning of the school year. One mathematics lesson in each classroom was videotaped to assess the teachers’ classroom management practices. Multilevel structural equation models revealed a positive relationship between student social behavior and their social acceptance in the peer group. Contrary to expectations, teachers’ attitudes toward inclusion did not predict their classroom management practices (i.e., implementation of clear rules and successful time management). As hypothesized, teachers’ classroom management predicted the level of social acceptance in the classroom, whereas teachers’ attitudes toward the inclusion of students with special educational needs did not. The study results are discussed in light of previous findings and implications for teacher education are described.
Protecting Healthcare Workers Amid the COVID-19 Crisis: A Safety Protocol in Wuhan
Authors: Liu Yunlu , Yang Shijun , Hung Man , Tong Wei , Liu Yong
Published: October 2020

Coronavirus disease 2019 (COVID-19), which is caused by a distinct coronavirus, is an acute infectious disease that spreads mainly via the respiratory route. During the COVID-19 outbreak, many healthcare systems faced a severe burden when a large number of healthcare workers (HCWs) became infected due to the lack of adequate protection. Consequently, it was apparent that it is important to ensure the health and safety of HCWs in order to control the outbreak throughout society. In this article, we share our successful protocol for protecting the safety of HCWs in the course of their daily work in an orthopedics department with the aim of eventually reducing the risk of nosocomial infection. None of our HCWs or their families contracted the infection during the COVID-19 pandemic.
Influence of Farming System on Weed Infestation and on Productivity of Narrow-Leaved Lupin (Lupinus angustifolius L.)
Authors: Faligowska Agnieszka , Panasiewicz Katarzyna , Szymańska Grażyna , Ratajczak Karolina , Sulewska Hanna , Pszczółkowska Agnieszka , Kocira Anna
Published: October 2020

Legumes have become important crops, due to an increasing global population and its demand for feed protein. Furthermore, legumes can improve the characteristics of the soil, improve biodiversity levels in crop rotations, and be cultivated in both organic and sustainable farming systems. In this study, a two-factor field experiment was conducted in Gorzyń, Poland in 2011–2015. The first factor was the farming system: low-external inputs (LI; without fertilization and chemical protection), medium-input (MI; medium fertilization level and chemical protection), and high-input (conventional—CONV; high fertilization level and chemical protection). Narrow-leaved lupin cultivar was the second factor; the indeterminate cv. Kalif and the determinate cv. Regent. We evaluated (a) weed infestation levels, (b) seed and protein production, and (c) the economic effects of narrow-leaved lupin cultivation under different farming conditions. A total of 12 weed species were identified, with the lowest weed density level and biomass production observed in CONV, and the greatest weed density level observed in LI. Seed yield was determined by the farming system; the greatest in CONV and significantly lower in LI (by 0.73 t h−1) and MI (by 0.18 t ha–1). Little difference was observed in seed yield between cultivars. The greatest production values for the Kalif and Regent cultivars (996€ and 949€ ha–1, respectively) were recorded in CONV, although LI proved to be the most profitable (with the highest gross agricultural income and lowest total cost of production). LI farming systems, in conjunction with chemical weed control, should be investigated in future studies.
Landscape Features Associated with Damage to Maize (Zea mays) Fields in Central México: A Comparison of Wind and Wildlife Damage
Authors: Flores-Armillas Victor Hugo , López-Medellín Xavier , García Barrios Raúl , MacGregor-Fors Ian , Valenzuela-Galván David
Published: October 2020

Damage to maize (Zea mays) fields leads to negative attitudes towards wildlife that can affect conservation. In a Biosphere Reserve in central Mexico, local inhabitants perceive that wildlife causes major damage to maize fields. Therefore, we quantified maize damaged by wildlife species and by wind, and we explored whether this was related to the proportion of land-use categories in the landscape surrounding maize fields and the distance from maize fields to the nearest human settlements, water sources and forest patches. We quantified damaged maize plants and cobs in 180 samples from six 100 m2 quadrats in each of 30 maize fields. On average, damage in maize fields was less than 2% and 6.5% of the total maize cobs and plants, respectively. The white-nosed coati (Nasua narica) and the white-tailed deer (Odocoileus virginianus) were responsible for most of the total damage to maize cobs, while wind was responsible for most of the damage to plants. Classification and regression tree analyses (CARTs) revealed that the highest levels of maize cob damage occurred in maize fields with less agricultural land cover in the surrounding landscape units and with longer distances to the nearest villages. Measured levels of maize damage were lower than the amount of damage perceived by local inhabitants. This human–wildlife conflict should be further addressed in the study area to avoid negative consequences for wildlife conservation.
Analysis of Derivatized N-Acyl Amino Acid Surfactants Using HPLC and HPLC/MS
Authors: Valigurová Petra , Jarešová Lenka , Váňová Jana , Česla Petr
Published: October 2020

A method for the analysis of weak anionic surfactants based on N-acyl amino acids was developed. The surfactants were derivatized using 2,4′-dibromoacetophenone yielding 4′-bromophenacyl esters suitable for spectrophotometric detection. Surfactants containing glycine, threonine and glutamic acid were analyzed after derivatization using reversed-phase liquid chromatography with UV/Vis and MS detection. The gradient profile was optimized using isocratic retention data of N-acyl-linked fatty acid homologues. The relative content of the homologues of N-acyl-linked fatty acids was expressed using the determined method. The intraday repeatability and stability of the prepared derivatives was tested. The relative content of fatty acids in the surfactants was correlated with the most common sources of fatty acids, showing high Pearson’s correlation coefficients with the typical fatty acids profile of a coconut oil.
Combination Therapy Using Low-Concentration Oxacillin with Palmitic Acid and Span85 to Control Clinical Methicillin-Resistant Staphylococcus aureus
Authors: Song Hun-Suk , Choi Tae-Rim , Bhatia Shashi Kant , Lee Sun Mi , Park Sol Lee , Lee Hye Soo , Kim Yun-Gon , Kim Jae-Seok , Kim Wooseong , Yang Yung-Hun
Published: October 2020

The overuse of antibiotics has led to the emergence of multidrug-resistant bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA). MRSA is difficult to kill with a single antibiotic because it has evolved to be resistant to various antibiotics by increasing the PBP2a (mecA) expression level, building up biofilm, introducing SCCmec for multidrug resistance, and changing its membrane properties. Therefore, to overcome antibiotic resistance and decrease possible genetic mutations that can lead to the acquisition of higher antibiotic resistance, drug combination therapy was applied based on previous results indicating that MRSA shows increased susceptibility to free fatty acids and surfactants. The optimal ratio of three components and the synergistic effects of possible combinations were investigated. The combinations were directly applied to clinically isolated strains, and the combination containing 15 μg/mL of oxacillin was able to control SCCmec type III and IV isolates having an oxacillin minimum inhibitory concentration (MIC) up to 1024 μg/mL; moreover, the combination with a slightly increased oxacillin concentration was able to kill SCCmec type II. Phospholipid analysis revealed that clinical strains with higher resistance contained a high portion of 12-methyltetradecanoic acid (anteiso-C15:0) and 14-methylhexadecanoic acid (anteiso-C17:0), although individual strains showed different patterns. In summary, we showed that combinatorial therapy with a low concentration of oxacillin controlled different laboratory and highly diversified clinical MRSA strains.
Synthesis of Normal Heart Sounds Using Generative Adversarial Networks and Empirical Wavelet Transform
Authors: Narváez Pedro , Percybrooks Winston S.
Published: October 2020

Currently, there are many works in the literature focused on the analysis of heart sounds, specifically on the development of intelligent systems for the classification of normal and abnormal heart sounds. However, the available heart sound databases are not yet large enough to train generalized machine learning models. Therefore, there is interest in the development of algorithms capable of generating heart sounds that could augment current databases. In this article, we propose a model based on generative adversary networks (GANs) to generate normal synthetic heart sounds. Additionally, a denoising algorithm is implemented using the empirical wavelet transform (EWT), allowing a decrease in the number of epochs and the computational cost that the GAN model requires. A distortion metric (mel–cepstral distortion) was used to objectively assess the quality of synthetic heart sounds. The proposed method was favorably compared with a mathematical model that is based on the morphology of the phonocardiography (PCG) signal published as the state of the art. Additionally, different heart sound classification models proposed as state-of-the-art were also used to test the performance of such models when the GAN-generated synthetic signals were used as test dataset. In this experiment, good accuracy results were obtained with most of the implemented models, suggesting that the GAN-generated sounds correctly capture the characteristics of natural heart sounds.
A 3-D Simulation of a Single-Sided Linear Induction Motor with Transverse and Longitudinal Magnetic Flux
Authors: Hernández Juan Antonio Domínguez , Carralero Natividad Duro , Vázquez Elena Gaudioso
Published: October 2020

This paper presents a novel and improved configuration of a single-sided linear induction motor. The geometry of the motor has been modified to be able to operate with a mixed magnetic flux configuration and with a new configuration of paths for the eddy currents induced inside the aluminum plate. To this end, two slots of dielectric have been introduced into the aluminum layer of the moving part with a dimension of 1 mm, an iron yoke into the primary part, and lastly, the width of the transversal slots has been optimized. Specifically, in the enhanced motor, there are two magnetic fluxes inside the motor that circulate across two different planes: a longitudinal magnetic flux which goes along the direction of the movement and a transversal magnetic flux which is closed through a perpendicular plane with respect to that direction. With this new configuration, the motor achieves a great increment of the thrust force without increasing the electrical supply. In addition, the proposed model creates a new spatial configuration of the eddy currents and an improvement of the main magnetic circuit. These novelties are relevant because they represent a great improvement in the efficiency of the linear induction motor for low velocities at a very low cost. All simulations have been made with the finite elements method—3D, both in standstill conditions and in motion in order to obtain the characteristic curves of the main forces developed by the linear induction motor.
Robust Optimization Approaches for a Natural Pharmaceutical Complex Product of Atractylodes Japonica Koidz and Schisandra Chinensis
Authors: Jang Hyeon-ae , Kim Sun Young , Lim Yun Young , Lim Jong Lae , Shin Sangmun
Published: October 2020

Experimental results pertaining to natural pharmaceutical complex products (NPCPs) often exhibit large variabilities in their associated response variables. To improve the quality of an NPCP, systemic studies (i.e., statistical analysis and mathematical optimization), including variability analysis and robust optimization, are often required. To this end, a systemic approach for an NPCP development process is proposed by integrating robust design and optimization methodologies. A quality function deployment method can be used to systematically define a standardized manufacturing process and relevant process variables for Chong Kun Dang (CKD)-497. Based on those variables, an experiment is designed using response surface methodology to mathematically estimate the output response functions associated with input variables. In addition, a design space (DS), which can guarantee the quality of an NPCP, is demonstrated by utilizing the overlaid contour plots of the estimated response functions. Finally, a CKD-497 case study is conducted for verification and validation.
Advances in Electrochemical Aptasensors Based on Carbon Nanomaterials
Authors: Evtugyn Gennady , Porfireva Anna , Shamagsumova Rezeda , Hianik Tibor
Published: October 2020

Carbon nanomaterials offer unique opportunities for the assembling of electrochemical aptasensors due to their high electroconductivity, redox activity, compatibility with biochemical receptors and broad possibilities of functionalization and combination with other auxiliary reagents. In this review, the progress in the development of electrochemical aptasensors based on carbon nanomaterials in 2016–2020 is considered with particular emphasis on the role of carbon materials in aptamer immobilization and signal generation. The synthesis and properties of carbon nanotubes, graphene materials, carbon nitride, carbon black particles and fullerene are described and their implementation in the electrochemical biosensors are summarized. Examples of electrochemical aptasensors are classified in accordance with the content of the surface layer and signal measurement mode. In conclusion, the drawbacks and future prospects of carbon nanomaterials’ application in electrochemical aptasensors are briefly discussed.
Use of Growth-Rate/Temperature-Gradient Charts for Defect Engineering in Crystal Growth from the Melt
Authors: Duffar Thierry
Published: October 2020

As the requirements in terms of crystal defect/quality and production yield are generally contradictory, it is necessary to develop methods in order to find the best compromise for the growth conditions of a given crystal. Simple growth-rate/temperature-gradient charts are a possible tool in this respect. After the recall of the classical analytical equations useful for describing the process and defect engineering, a simple pedagogic case explains the building and use of such charts. The more complex application to the directional casting of photovoltaic Si necessitated the development of new physical models for twinning and equiaxed growth. This allowed plotting charts that proved useful for industrial applications. The conclusions discuss the drawbacks and advantages of the method. It finally proves to be a pedagogic tool for teaching crystal growth engineering.
A Study on the Application Possibility of the Vehicle Air Conditioning System Using Vortex Tube
Authors: Kim Younghyeon , Im Seokyeon , Han Jaeyoung
Published: October 2020

Since refrigerants applied to vehicle air conditioning systems exacerbate global warming, many studies have been conducted to supplement them. However, most studies have attempted to maximize the efficiency and minimize the environmental impact of the refrigerant, and thus, an air conditioning system without refrigerant is required. The vortex tube is a temperature separation system capable of separating air at low and high temperatures using compressed air. When applied to an air conditioning system, it is possible to construct an eco-friendly system that does not use a refrigerant. In this paper, various temperature changes and characteristics of a vortex tube were identified and applied to an air conditioning system simulation device. Additionally, an air conditioning system simulation device using indirect heat exchange and direct heat exchange methods was constructed to test the low-temperature air flow rate (yc), according to the temperature and pressure. As a result of the experiment, the temperature of the indirect heat exchange method was found to be higher than the direct heat exchange method, but the direct heat exchange method had low flow resistance. As a result, the direct heat exchange method can easily control the temperature according to the pressure and the low-temperature air flow rate (yc). Therefore, it was judged that the direct heat exchange method is more feasible for use in air conditioning systems than the indirect heat exchange method.
Clarke Transformation Solution of Asymmetrical Transients in Three-Phase Circuits
Authors: Bellan Diego
Published: October 2020

This work deals with the use of the Clarke transformation for the theoretical derivation of circuit models for the analysis of asymmetrical transients in three-phase circuits. Asymmetrical transients occur when only one or two phases of a three-phase power system are involved in a switch operation. Such a condition is critical from a theoretical viewpoint since the Clarke transformation is based on the assumption of circuit symmetry between the three phases. If the symmetry assumption is not met, the equivalent circuits in the transformed variables α, β, and 0 are not uncoupled. The literature concerning numerical approaches for asymmetrical transient analysis is very rich, but a comprehensive and rigorous analytical investigation of circuit models within the framework of the Clarke transformation is still lacking. Contrary to numerical approaches, analytical solutions provide deeper insight into the phenomenon and allow for theoretical interpretation and better understanding of the transient behavior. The proposed circuit models show that the β variables are always uncoupled with α and 0 variables, whereas coupling between α and 0 variables can be properly represented by an ideal transformer. Moreover, in the case of single-line switching, the β variables have no transient, i.e., they keep the steady-state behavior. Transient properties can be readily and effectively observed by representing the trajectory of the space vector on the complex plane. All the analytical results have been checked numerically through the Simulink (Matlab R2020a, The MathWorks, Inc., Natick, MA, USA) implementation of a specific three-phase circuit introduced to illustrate the main theoretical issues.
Dynamic Stall Model for Tower Shadow Effects on Downwind Turbines and Its Scale Effects
Authors: Yoshida Shigeo
Published: October 2020

A dynamic stall model for tower shadow effects is developed for downwind turbines. Although Munduate’s model shows good agreement with a 1.0 m wind tunnel test model, two problems exist: (1) it does not express load increase before the entrance of the tower wake, and (2) it uses the empirical tower wake model to determine the wind speed profile behind the tower. The present research solves these problems by combining Moriarty’s tower wake model and the entrance condition of the tower wake. Moriarty’s model does not require any empirical parameter other than tower drag coefficient and it expresses positive wind speed around the tower also. Positive wind speed change is also allowed as the tower wake entrance condition in addition to the negative change observed in the previous model. It demonstrates better agreement with a wind tunnel test and contributes to the accuracy of the fatigue load, as it expresses a slight increase in load around the entrance of the tower wake. Furthermore, the scale effects are also evaluated; lift deviation becomes smaller as the scale increases, i.e., lower rotor speed.
Effect of Citrus By-product on Physicochemical Parameters, Sensory Analysis and Volatile Composition of Different Kinds of Cheese from Raw Goat Milk
Authors: Guzmán José Luis , Delgado Pertíñez Manuel , Galán Soldevilla Hortensia , Ruiz Pérez-Cacho Pilar , Polvillo Polo Oliva , Zarazaga Luis Ángel , Avilés Ramírez Carmen
Published: October 2020

The increased use of concentrates to reduce pasture as a feed source in productive systems like Payoya breed goat farms has made it necessary to decrease feeding costs. The inclusion of agro-industry by-products such as dry orange pulp pellets in goat diets has been suggested as a sustainable alternative to cereal-based concentrates. The aim of this work was to assess the influence of diets including dry orange pulp pellets on the quality of cheeses traditionally made from Payoya breed goat milk. We analysed the physicochemical characteristics, sensory properties and volatile compound profiles of 18 artisanal cheeses made from raw Payoya milk. In this study, goats were fed with different concentrations of dry orange pulp; and cheeses were curdled with animal and vegetable coagulants. Slight differences were detected between some cheeses. However, the use of citrus by-products in the Payoya goat diets did not substantially affect the cheeses’ physicochemical properties, olfactory attributes, or volatile profiles. Therefore, dried citrus pulp can be used as a substitute for cereal concentrates without affecting the distinct properties of these ripened raw goat milk cheeses.
Microbiological Quality of Ready-to-Eat Leafy Green Salads during Shelf-Life and Home-Refrigeration
Authors: Arienzo Alyexandra , Murgia Lorenza , Fraudentali Ilaria , Gallo Valentina , Angelini Riccardo , Antonini Giovanni
Published: October 2020

The market of ready-to-eat leafy green salads is experiencing a noticeable growth in Europe. Since they are intended to be consumed without additional treatments, these ready-to-eat products are associated with a high microbiological risk. The aim of this work was to evaluate the microbiological quality and safety of ready-to-eat leafy green salads sold in widespread supermarket chains in Lazio, Italy, on the packaging date during shelf-life and during home-refrigeration. The study also aimed to determine the differences between low-, medium-, and high-cost products. Salmonella spp. and L. monocytogenes were chosen as safety indicators as specified by European regulations while total aerobic mesophilic bacteria and Escherichia coli were chosen as quality indicators as suggested by national guidelines. Analyses were performed following the ISO standards and in parallel for the evaluation of total aerobic mesophilic bacteria, with an alternative colorimetric system, the Micro Biological Survey method, in order to propose a simple, affordable and accurate alternative for testing the microbiological quality of products, especially suitable for small and medium enterprises and on-site analyses. The study revealed high, unsatisfactory, total bacterial loads in all analyzed samples on the packaging date and expiry date and a very high prevalence of Salmonella spp. (67%) regardless of the selected varieties and cost categories; L. monocytogenes was not recovered aligning with the results obtained in other studies.
Analysis of Volatile Compounds in Soju, a Korean Distilled Spirit, by SPME-Arrow-GC/MS
Authors: Cha Jiyoon , Chin Young-Wook , Lee Jun-Young , Kim Tae-Wan , Jang Hae Won
Published: October 2020

The SPME Arrow technology—a novel solid phase micro-extraction technique—was used to analyze Soju, a traditional Korean distilled liquor, in barrels made of Quercus spp. The volatile compounds detected when the barrels were toasted were analyzed. Five types of sorbents—carbon wide range/polydimethylsiloxane, divinylbenzene/carbon wide range/polydimethylsiloxane, divinylbenzene/polydimethylsiloxane, polydimethylsiloxane, and polyacrylate—were used for this investigation. Fifty-four volatile compounds were detected in Soju using gas chromatography/mass spectrometry. A high extraction efficiency was obtained using carbon wide range/polydimethylsiloxane. Nineteen samples were analyzed using barrels made of six species of carbonated oak (Q. aliena, Q. variabilis, Q. dentate, Q. acutissima, Q. mongolica, and Q. serrata) and control groups in three ways: noncharring, medium charring, and heavy charring. Ethanol, 1-propanol, isoamyl acetate, and isoamyl alcohol can be used as indicator volatile components for Soju and other such traditional Korean distilled liquors. We believe our study results can be used to design better analysis methods for Soju and other distilled liquors.
Teaching Geriatrics and Transitions of Care to Internal Medicine Resident Physicians
Authors: Wu Shirley , Jackson Nicholas , Larson Spencer , Ward Katherine T.
Published: October 2020

(1) Background: Internal medicine (IM) resident physicians need to be trained to care for older adults and transition them safely across care settings. Objective: The study purpose was to evaluate the efficacy of a curriculum in geriatrics assessment and communication skills for transitions of care (TOCs) to IM resident physicians. (2) Methods: IM residents rotated for 4 weeks on the geriatrics consult service at a large public teaching hospital, where they received didactic lectures and clinical experience in consultation and transitional care. The curriculum was designed to meet consensus guidelines for minimum geriatrics competencies expected of IM residents. Previously validated and published assessment tools were used for geriatrics knowledge and attitudes. Locally developed tools were used to directly observe and rate communication skills, and self-assess geriatrics assessment and health literacy skills. The curriculum was evaluated using a quasi-experimental, nonrandomized, single-group pre- and post-test observational design. Data on 31 subjects were collected over 18 months and analyzed using mixed-effects models. (3) Results: Average knowledge scores improved from 65% to 74% (Δ9%, 95% CI 4–13%, p < 0.001). Communication skills improved by an average of 1.15 points (95% CI 0.66–1.64, p < 0.001) on a 9-point scale. Attitudes did not change significantly. Self-rated confidence in geriatrics assessment and health literacy skills improved modestly. (4) Conclusions: The curriculum is effective in teaching basic geriatrics knowledge and communication skills, and increasing self-confidence in geriatrics assessment skills. In settings where an inpatient geriatrics consult service is feasible, the curriculum may be a model for combining geriatrics and TOC training.
Re-Sounding Alarms: Designing Ergonomic Auditory Interfaces by Embracing Musical Insights
Authors: Foley Liam , Anderson Cameron J. , Schutz Michael
Published: October 2020

Auditory alarms are an important component of human–computer interfaces, used in mission-critical industries such as aviation, nuclear power plants, and hospital settings. Unfortunately, problems with recognition, detection, and annoyance continue to hamper their effectiveness. Historically, they appear designed more in response to engineering constraints than principles of hearing science. Here we argue that auditory perception in general and music perception in particular hold valuable lessons for alarm designers. We also discuss ongoing research suggesting that the temporal complexity of musical tones offers promising insight into new ways of addressing widely recognized shortcomings of current alarms.
N-Butylidenephthalide Inhibits the Phenotypic Switch of VSMCs through Activation of AMPK and Prevents Stenosis in an Arteriovenous Fistula Rat Model
Authors: Yang Hsin-Han , Xu Yue-Xuan , Chen Jie-Yi , Harn Horng-Jyh , Chiou Tzyy-Wen
Published: October 2020

The phenotypic switch of vascular smooth muscle cells (VSMCs) plays a pivotal role in the development of vascular disorders, such as atherosclerosis, stenosis and restenosis, after vascular intervention. In our previous study, n-butylidenephthalide (BP) was reported to have anti-proliferating and apoptotic effects on VSMCs. The purpose of the current study is to further investigate its role in platelet-derived growth factor (PDGF)-induced VSMC phenotypic modulation in an arteriovenous fistula model. In vitro, we observed that BP inhibited the PDGF-induced cytoskeleton reorganization of the VSMCs. The enhanced expression of vimentin and collagen, as well as the migration ability induced by PDGF, were also inhibited by BP. By cell cycle analysis, we found that BP inhibited the PDGF-induced VSMCs proliferation and arrested the VSMCs in the G0/G1 phase. In an arteriovenous fistula rat model, the formation of stenosis, which was coupled with a thrombus, and the expression of vimentin and collagen in VSMCs, were also inhibited by administration of BP, indicating that BP inhibited the PDGF-induced phenotypic switch and the migration of VSMCs. Besides, the inhibitory effects of BP on the phenotypic switch were found to accompany the activated 5’ AMP-activated protein kinase (AMPK) as well as the inhibited phosphorylation of mTOR. Knockdown of AMPK by gene silencing conflicted the effects of BP and further exacerbated the PDGF-induced VSMCs phenotypic switch, confirming the modulating effect that BP exerted on the VSMCs by this pathway. These findings suggest that BP may contribute to the vasculoprotective potential in vasculature.
Effect of Vesicle Size on the Cytolysis of Cell-Penetrating Peptides (CPPs)
Authors: Sakamoto Kazutami , Kitano Takeshi , Kuwahara Haruka , Tedani Megumi , Aburai Kenichi , Futaki Shiroh , Abe Masahiko , Sakai Hideki , Ohtaka Hiroyasu , Yamashita Yuji
Published: October 2020

A specific series of peptides, called a cell-penetrating peptide (CPP), is known to be free to directly permeate through cell membranes into the cytosol (cytolysis); hence, this CPP would be a potent carrier for a drug delivery system (DDS). Previously, we proposed the mechanism of cytolysis as a temporal and local phase transfer of membrane lipid caused by positive membrane curvature generation. Moreover, we showed how to control the CPP cytolysis. Here, we investigate the phospholipid vesicle’s size effect on CPP cytolysis because this is the most straightforward way to control membrane curvature. Contrary to our expectation, we found that the smaller the vesicle diameter (meaning a higher membrane curvature), the more cytolysis was suppressed. Such controversial findings led us to seek the reason for the unexpected results, and we ended up finding out that the mobility of membrane lipids as a liquid crystal is the key to cytolysis. As a result, we could explain the cause of cytolysis suppression by reducing the vesicle size (because of the restriction of lipid mobility); osmotic pressure reduction to enhance positive curvature generation works as long as the membrane is mobile enough to modulate the local structure. Taking all the revealed vital factors and their effects as a tool, we will further explore how to control CPP cytolysis for developing a DDS system combined with appropriate cargo selection to be tagged with CPPs.
Rose Bengal Crosslinking to Stabilize Collagen Sheets and Generate Modulated Collagen Laminates
Authors: Eckes Stefanie , Braun Joy , Wack Julia S. , Ritz Ulrike , Nickel Daniela , Schmitz Katja
Published: October 2020

For medical application, easily accessible biomaterials with tailored properties are desirable. Collagen type I represents a biomaterial of choice for regenerative medicine and tissue engineering. Here, we present a simple method to modify the properties of collagen and to generate collagen laminates. We selected three commercially available collagen sheets with different thicknesses and densities and examined the effect of rose bengal and green light collagen crosslinking (RGX) on properties such as microstructure, swelling degree, mechanical stability, cell compatibility and drug release. The highest impact of RGX was measured for Atelocollagen, for which the swelling degree was reduced from 630% (w/w) to 520% (w/w) and thickness measured under force application increased from 0.014 mm to 0.455 mm, indicating a significant increase in mechanical stability. Microstructural analysis revealed that the sponge-like structure was replaced by a fibrous structure. While the initial burst effect during vancomycin release was not influenced by crosslinking, RGX increased cell proliferation on sheets of Atelocollagen and on Collagen Solutions. We furthermore demonstrate that RGX can be used to covalently attach different sheets to create materials with combined properties, making the modification and combination of readily available sheets with RGX an attractive approach for clinical application.
Self-Replication of Prion Protein Fragment 89-230 Amyloid Fibrils Accelerated by Prion Protein Fragment 107-143 Aggregates
Authors: Sneideris Tomas , Ziaunys Mantas , Chu Brett K.-Y. , Chen Rita P.-Y. , Smirnovas Vytautas
Published: October 2020

Prion protein amyloid aggregates are associated with infectious neurodegenerative diseases, known as transmissible spongiform encephalopathies. Self-replication of amyloid structures by refolding of native protein molecules is the probable mechanism of disease transmission. Amyloid fibril formation and self-replication can be affected by many different factors, including other amyloid proteins and peptides. Mouse prion protein fragments 107-143 (PrP(107-143)) and 89-230 (PrP(89-230)) can form amyloid fibrils. β-sheet core in PrP(89-230) amyloid fibrils is limited to residues ∼160–220 with unstructured N-terminus. We employed chemical kinetics tools, atomic force microscopy and Fourier-transform infrared spectroscopy, to investigate the effects of mouse prion protein fragment 107-143 fibrils on the aggregation of PrP(89-230). The data suggest that amyloid aggregates of a short prion-derived peptide are not able to seed PrP(89-230) aggregation; however, they accelerate the self-replication of PrP(89-230) amyloid fibrils. We conclude that PrP(107-143) fibrils could facilitate the self-replication of PrP(89-230) amyloid fibrils in several possible ways, and that this process deserves more attention as it may play an important role in amyloid propagation.
p38 MAPK Pathway in the Heart: New Insights in Health and Disease
Authors: Romero-Becerra Rafael , Santamans Ayelén , Folgueira Cintia , Sabio Guadalupe
Published: October 2020

The p38 mitogen-activated kinase (MAPK) family controls cell adaptation to stress stimuli. p38 function has been studied in depth in relation to cardiac development and function. The first isoform demonstrated to play an important role in cardiac development was p38α; however, all p38 family members are now known to collaborate in different aspects of cardiomyocyte differentiation and growth. p38 family members have been proposed to have protective and deleterious actions in the stressed myocardium, with the outcome of their action in part dependent on the model system under study and the identity of the activated p38 family member. Most studies to date have been performed with inhibitors that are not isoform-specific, and, consequently, knowledge remains very limited about how the different p38s control cardiac physiology and respond to cardiac stress. In this review, we summarize the current understanding of the role of the p38 pathway in cardiac physiology and discuss recent advances in the field.
Structural and Computational Insights into a Blebbistatin-Bound Myosin•ADP Complex with Characteristics of an ADP-Release Conformation along the Two-Step Myosin Power Stoke
Authors: Ewert Wiebke , Franz Peter , Tsiavaliaris Georgios , Preller Matthias
Published: October 2020

The motor protein myosin drives a wide range of cellular and muscular functions by generating directed movement and force, fueled through adenosine triphosphate (ATP) hydrolysis. Release of the hydrolysis product adenosine diphosphate (ADP) is a fundamental and regulatory process during force production. However, details about the molecular mechanism accompanying ADP release are scarce due to the lack of representative structures. Here we solved a novel blebbistatin-bound myosin conformation with critical structural elements in positions between the myosin pre-power stroke and rigor states. ADP in this structure is repositioned towards the surface by the phosphate-sensing P-loop, and stabilized in a partially unbound conformation via a salt-bridge between Arg131 and Glu187. A 5 Å rotation separates the mechanical converter in this conformation from the rigor position. The crystallized myosin structure thus resembles a conformation towards the end of the two-step power stroke, associated with ADP release. Computationally reconstructing ADP release from myosin by means of molecular dynamics simulations further supported the existence of an equivalent conformation along the power stroke that shows the same major characteristics in the myosin motor domain as the resolved blebbistatin-bound myosin-II·ADP crystal structure, and identified a communication hub centered on Arg232 that mediates chemomechanical energy transduction.
Intraarticular Administration Effect of Hydrogen Sulfide on an In Vivo Rat Model of Osteoarthritis
Authors: Vaamonde-García Carlos , Burguera Elena F. , Vela-Anero Ángela , Hermida-Gómez Tamara , Filgueira-Fernández Purificación , Fernández-Rodríguez Jennifer A. , Meijide-Faílde Rosa , Blanco Francisco J.
Published: October 2020

Osteoarthritis (OA) is the most common articular chronic disease. However, its current treatment is limited and mostly symptomatic. Hydrogen sulfide (H2S) is an endogenous gas with recognized physiological activities. The purpose here was to evaluate the effects of the intraarticular administration of a slow-releasing H2S compound (GYY-4137) on an OA experimental model. OA was induced in Wistar rats by the transection of medial collateral ligament and the removal of the medial meniscus of the left joint. The animals were randomized into three groups: non-treated and intraarticularly injected with saline or GYY-4137. Joint destabilization induced articular thickening (≈5% increment), the loss of joint mobility and flexion (≈12-degree angle), and increased levels of pain (≈1.5 points on a scale of 0 to 3). Animals treated with GYY-4137 presented improved motor function of the joint, as well as lower pain levels (≈75% recovery). We also observed that cartilage deterioration was attenuated in the GYY-4137 group (≈30% compared with the saline group). Likewise, these animals showed a reduced presence of pro-inflammatory mediators (cyclooxygenase-2, inducible nitric oxide synthase, and metalloproteinase-13) and lower oxidative damage in the cartilage. The increment of the nuclear factor-erythroid 2-related factor 2 (Nrf-2) levels and Nrf-2-regulated gene expression (≈30%) in the GYY-4137 group seem to be underlying its chondroprotective effects. Our results suggest the beneficial impact of the intraarticular administration of H2S on experimental OA, showing a reduced cartilage destruction and oxidative damage, and supporting the use of slow H2S-producing molecules as a complementary treatment in OA.
Comprehensive Analysis of Antibodies Induced by Vaccination with 4 Kinds of Avian Influenza H5N1 Pre-Pandemic Vaccines
Authors: Ohshima Nobuko , Iba Yoshitaka , Kubota-Koketsu Ritsuko , Yamasaki Ayami , Majima Keiko , Kurosawa Gene , Hirano Daisuke , Yoshida Shunji , Sugiura Mototaka , Asano Yoshizo , Okuno Yoshinobu , Kurosawa Yoshikazu
Published: October 2020

Four kinds of avian-derived H5N1 influenza virus, A/Vietnam/1194/2004 (Clade 1), A/Indonesia/5/2005 (Clade 2.1), A/Qinghai/1A/2005 (Clade 2.2), and A/Anhui/1/2005 (Clade 2.3), have been stocked in Japan for use as pre-pandemic vaccines. When a pandemic occurs, these viruses would be used as vaccines in the hope of inducing immunity against the pandemic virus. We analyzed the specificity of antibodies (Abs) produced by B lymphocytes present in the blood after immunization with these vaccines. Eighteen volunteers took part in this project. After libraries of Ab-encoding sequences were constructed using blood from subjects vaccinated with these viruses, a large number of clones that encoded Abs that bound to the virus particles used as vaccines were isolated. These clones were classified into two groups according to the hemagglutination inhibition (HI) activity of the encoded Abs. While two-thirds of the clones were HI positive, the encoded Abs exhibited only restricted strain specificity. On the other hand, half of the HI-negative clones encoded Abs that bound not only to the H5N1 virus but also to the H1N1 virus; with a few exceptions, these Abs appeared to be encoded by memory B cells present before vaccination. The HI-negative clones included those encoding broadly cross-reactive Abs, some of which were encoded by non-VH1-69 germline genes. However, although this work shows that various kinds of anti-H5N1 Abs are encoded by volunteers vaccinated with pre-pandemic vaccines, broad cross-reactivity was seen only in a minority of clones, raising concern regarding the utility of these H5N1 vaccine viruses for the prevention of H5N1 pandemics.
Wheat and Barley: Acclimatization to Abiotic and Biotic Stress
Authors: Hura Tomasz
Published: October 2020

Twelve articles (ten research papers and two reviews) included in the Special Issue entitled “Wheat and Barley: Acclimatization to Abiotic and Biotic Stress” are summed up here to present the latest research on the molecular background of adaptation to environmental stresses in two cereal species. Crucial research results were presented and discussed, as they may be of importance in breeding aimed at increasing wheat and barley tolerance to abiotic and biotic stresses.