bluuebunny/crossref_metadata_2025 huggingface dataset:
Polymorphism of Genes Potentially Affecting Growth and Body Size Suggests Genetic Divergence in Wild and Domestic Reindeer (Rangifer tarandus) Populations
Authors: Krutikova Anna , Dementieva Natalia , Shcherbakov Yuri , Goncharov Vasiliy , Griffin Darren , Romanov Michael
Published: December 2024

Background/Objectives: A combination of increased human presence in the Arctic zone alongside climate change has led to a decrease in the number of wild reindeer (Rangifer tarandus). Studying the genetic potential of this species will aid in conservation efforts, while simultaneously promoting improved meat productivity in domestic reindeer. Alongside reducing feed costs, increasing disease resistance, etc., acquiring genetic variation information is a crucial task for domestic reindeer husbandry. This study thus identified highly informative molecular genetic markers usable for assessing genetic diversity and breeding purposes in reindeer. Methods: We analyzed gene polymorphism that may potentially affect animal growth and development in populations of wild (Taimyr Peninsula) and domestic reindeer, including Nenets and Evenk breeds. We screened these populations for polymorphisms by sequencing the GH, GHR, LCORL and BMP2 genes. Results: Following generation of gene sequences, we compared the alleles frequency in the surveyed populations and their genetic divergence. Some loci lacked polymorphism in wild reindeer, unlike domestic breeds. This could suggest a selection-driven microevolutionary divergence in domestic reindeer populations. An isolated domestic population from Kolguyev Island appeared to be genetically remote from continental reindeer. Conclusions: Molecular genetic markers associated with economically important traits in reindeer can be further developed using the data obtained. Monitoring wild reindeer populations and better utilizing the genetic potential of domestic animals will depend on a panel of these marker genes. By using this marker panel, the amount of time spent on selection efforts will be greatly reduced to enhance meat performance during reindeer breeding.
Dynamic Chromatin Accessibility and Gene Expression Regulation During Maize Leaf Development
Authors: Wang Yiduo , Wang Shuai , Wu Yufeng , Cheng Jiawen , Wang Haiyan
Published: December 2024

Background/Objectives: Chromatin accessibility is closely associated with transcriptional regulation during maize (Zea mays) leaf development. However, its precise role in controlling gene expression at different developmental stages remains poorly understood. This study aimed to investigate the dynamics of chromatin accessibility and its influence on genome-wide gene expression during the BBCH_11, BBCH_13, and BBCH_17 stages of maize leaf development. Methods: Maize leaves were collected at the BBCH_11, BBCH_13, and BBCH_17 developmental stages, and chromatin accessibility was assessed using ATAC-seq. RNA-seq was performed to profile gene expression. Integrated analysis of ATAC-seq and RNA-seq data was conducted to elucidate the relationship between chromatin accessibility and transcriptional regulation. Results: A total of 46,808, 38,242, and 41,084 accessible chromatin regions (ACRs) were identified at the BBCH_11, BBCH_13, and BBCH_17 stages, respectively, with 23.4%, 12.2%, and 21.9% of these regions located near transcription start sites (TSSs). Integrated analyses revealed that both the number and intensity of ACRs significantly influence gene expression levels. Motif analysis identified key transcription factors associated with leaf development and potential transcriptional repressors among genes, showing divergent regulation patterns in ATAC-seq and RNA-seq datasets. Conclusions: These findings demonstrate that chromatin accessibility plays a crucial role in regulating the spatial and temporal expression of key genes during maize leaf development by modulating transcription factor binding. This study provides novel insights into the regulatory mechanisms underlying maize leaf development, contributing to a deeper understanding of chromatin-mediated gene expression.
Psychological Impact of Hospital Discharge on the Older Person: A Systematic Review
Authors: Hussein Yasmin , Edwards Sarah , Patel Harnish P
Published: December 2024

Introduction: Hospitalisation and prolonged length of stay is associated with deconditioning that risks adverse outcomes after discharge. Less is known about the psychological impact on older people after hospital discharge. The purpose of this systematic review was to elucidate factors contributing to psychological stress in older patients post-discharge to inform better discharge planning. Methods: A systematic search for studies reporting poor discharge outcomes in older people between 2010 and 2022 was performed in Medline, CINAHL, and PsycINFO. Search terms were ‘older patients > 65 year’, ‘post-discharge’, ‘psychological distress’, ‘loneliness’, ‘anxiety’, ‘depression’, and ‘length of hospital stay’. Exclusion criteria included COVID-19 disease, dementia (±severe cognitive impairment), individuals aged <65, and those under palliative care services. Results: A total of 1666 records were identified, of which 878 were excluded as they were outside of our date limits or were not written in the English language, 681 were excluded after application of exclusion criteria, and 699 were excluded because of insufficient details. A total of 31 duplicates were removed, leaving 38 articles that were assessed for eligibility; 7 of these reports were found suitable, comprising 1131 patients. Three highly relevant themes identified relating to post-discharge outcomes were social isolation, lack of support, depression and anxiety. Older patients with a tendency toward depressive symptoms had an increased likelihood of death. Conclusions: It appears that the discharge process from hospital fails to address psychological factors that permit a successful transition from hospital. Pre-discharge screening of psychological symptoms and coping ability may assist in identifying older patients who are at risk of mental as well as subsequent physical deterioration. Better knowledge of positive and negative predictors of a successful transition from hospital to home would enable more holistic, effective, and inclusive discharge planning processes for older adults.
Comprehensive Gerontological Assessment: An Update on the Concept and Its Evaluation Tools in Latin America and the Caribbean—A Literature Review
Authors: Pizarro-Mena Rafael , Rotarou Elena S. , Chavarro-Carvajal Diego , Wachholz Patrick Alexander , López María Fernanda , Perdomo Delgado Cristina , Parra-Soto Solange , Barrientos-Calvo Isabel , Retamal-Walter Felipe , Riveros-Basoalto Gloria
Published: December 2024

In recent decades, Latin America and the Caribbean region have experienced accelerated aging. However, despite the significant progress of gerontology in the region, the number of geriatricians and professionals trained in the field is low; a notable gap in the training related to the assessment of older adults can also be observed. Therefore, in this literature review, we update the concept of Comprehensive Gerontological Assessment (CGA) for its application in the region. We compile the characteristics, validity, and reliability of CGA tools, and their usage in government documents across countries in the region. We also analyze the adaptations made to CGA during the COVID-19 pandemic, and discuss challenges related to CGA administration, academic training, research, innovation, and management. This study is particularly relevant as it proposes lines of action for decision-makers, academics, researchers, university students, and the general community, which will allow for more tailored interventions aimed at meeting the needs of older adults, their families, and caregivers. Such actions will positively impact functionality, autonomy, and quality of life, while promoting healthy, active, and successful aging in the region.
Artificial Biopolymers Derived from Transgenic Plants: Applications and Properties—A Review
Authors: Latour-Paczka Krystyna , Luciński Robert
Published: December 2024

Biodegradable materials are currently one of the main focuses of research and technological development. The significance of these products grows annually, particularly in the fight against climate change and environmental pollution. Utilizing artificial biopolymers offers an opportunity to shift away from petroleum-based plastics with applications spanning various sectors of the economy, from the pharmaceutical and medical industries to food packaging. This paper discusses the main groups of artificial biopolymers. It emphasizes the potential of using genetically modified plants for its production, describing the primary plant species involved in these processes and the most common genetic modifications. Additionally, the paper explores the potential applications of biobased polymers, highlighting their key advantages and disadvantages in specific context.
Mucosal Bacterial Immunotherapy Attenuates the Development of Experimental Colitis by Reducing Inflammation Through the Regulation of Myeloid Cells
Authors: Jiménez Eva , Vázquez Alberto , González Sara , Sacedón Rosa , Fernández-Sevilla Lidia M. , Varas Alberto , Subiza Jose L. , Valencia Jaris , Vicente Ángeles
Published: December 2024

Ulcerative colitis is a chronic relapsing–remitting and potentially progressive form of inflammatory bowel disease in which there is extensive inflammation and mucosal damage in the colon and rectum as a result of an abnormal immune response. MV130 is a mucosal-trained immunity-based vaccine used to prevent respiratory tract infections in various clinical settings. Additionally, MV130 may induce innate immune cells that acquire anti-inflammatory properties and promote tolerance, which could have important implications for chronic inflammatory diseases such as ulcerative colitis. This work demonstrated that the prophylactic administration of MV130 substantially mitigated colitis in a mouse model of acute colitis induced by dextran sulphate sodium. MV130 downregulated systemic and local inflammatory responses, maintained the integrity of the intestinal barrier by preserving the enterocyte layer and goblet cells, and reduced the oedema and fibrosis characteristic of the disease. Mechanistically, MV130 significantly reduced the infiltration of neutrophils and pro-inflammatory macrophages in the intestinal wall of the diseased animals and favoured the appearance of M2-polarised macrophages. These results suggest that MV130 might have therapeutic potential for the treatment of ulcerative colitis, reducing the risk of relapse and the progression of disease.
Use of Gain-of-Function Screening to Identify miRNAs Involved in Paclitaxel Resistance in Triple-Negative Breast Cancer
Authors: Nemours Stéphane , Solé Carla , Goicoechea Ibai , Armesto María , Arestin María , Urruticoechea Ander , Rezola Marta , López Isabel Álvarez , Schaapveld Roel , Schultz Iman , Zhang Lei , Lawrie Charles H.
Published: December 2024

Paclitaxel is a widely used chemotherapeutic agent for the treatment of breast cancer (BC), including as a front-line treatment for triple-negative breast cancer (TNBC) patients. However, resistance to paclitaxel remains one of the major causes of death associated with treatment failure. Multiple studies have demonstrated that miRNAs play a role in paclitaxel resistance and are associated with both disease progression and metastasis. In the present study, we used a miRNA-encoding lentiviral library as a gain-of-function screen for paclitaxel resistance in the MDA-MB-231 TNBC cell line. We identified that miR-181b, miR-29a, miR-30c, miR-196 and miR-1295 conferred a resistant phenotype to cells. The expression of miR-29a also induced resistance to eribulin and vinorelbine, while miR-181b and miR-30c induced resistance to vinorelbine. We measured the levels of these miRNAs in breast cancer patients and observed higher levels of miR-29a in treatment-refractory patients. Taken together, we suggest that miR-29a and miR-181b may be good candidates for miRNA inhibition to overcome resistance to chemotherapy.
Fine-Tuned Expression of Evolutionarily Conserved Signaling Molecules in the Ciona Notochord
Authors: Negrón-Piñeiro Lenny J. , Wu Yushi , Mehta Ravij , Maguire Julie E. , Chou Cindy , Lee Joyce , Dahia Chitra L. , Di Gregorio Anna
Published: December 2024

The notochord is an axial structure required for the development of all chordate embryos, from sea squirts to humans. Over the course of more than half a billion years of chordate evolution, in addition to its structural function, the notochord has acquired increasingly relevant patterning roles for its surrounding tissues. This process has involved the co-option of signaling pathways and the acquisition of novel molecular mechanisms responsible for the precise timing and modalities of their deployment. To reconstruct this evolutionary route, we surveyed the expression of signaling molecules in the notochord of the tunicate Ciona, an experimentally amenable and informative chordate. We found that several genes encoding for candidate components of diverse signaling pathways are expressed during notochord development, and in some instances, display distinctive regionalized and/or lineage-specific patterns. We identified and deconstructed notochord enhancers associated with TGF-β and Ctgf, two evolutionarily conserved signaling genes that are expressed dishomogeneously in the Ciona notochord, and shed light on the cis-regulatory origins of their peculiar expression patterns.
The Emerging Role of PCSK9 in the Pathogenesis of Alzheimer’s Disease: A Possible Target for the Disease Treatment
Authors: Testa Gabriella , Giannelli Serena , Staurenghi Erica , Cecci Rebecca , Floro Lucrezia , Gamba Paola , Sottero Barbara , Leonarduzzi Gabriella
Published: December 2024

Alzheimer’s disease (AD) is a multifactorial neurodegenerative disease mainly caused by β-amyloid (Aβ) accumulation in the brain. Among the several factors that may concur to AD development, elevated cholesterol levels and brain cholesterol dyshomeostasis have been recognized to play a relevant role. Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a protein primarily known to regulate plasma low-density lipoproteins (LDLs) rich in cholesterol and to be one of the main causes of familial hypercholesterolemia. In addition to that, PCSK9 is also recognized to carry out diverse important activities in the brain, including control of neuronal differentiation, apoptosis, and, importantly, LDL receptors functionality. Moreover, PCSK9 appeared to be directly involved in some of the principal processes responsible for AD development, such as inflammation, oxidative stress, and Aβ deposition. On these bases, PCSK9 management might represent a promising approach for AD treatment. The purpose of this review is to elucidate the role of PCSK9, whether or not cholesterol-related, in AD pathogenesis and to give an updated overview of the most innovative therapeutic strategies developed so far to counteract the pleiotropic activities of both humoral and brain PCSK9, focusing in particular on their potentiality for AD management.
Immunomodulatory Effects of SPHK1 and Its Interaction with TFAP2A in Yellow Drum (Nibea albiflora)
Authors: Cui Yu , Luo Shuai , Wu Baolan , Li Qiaoying , Han Fang , Wang Zhiyong
Published: December 2024

Sphingosine kinases (SPHKs) are essential enzymes that catalyze the phosphorylation of sphingosine to produce sphingosine-1-phosphate (S1P), which plays pivotal roles in inflammation and immune regulation. In this study, genome-wide association analysis (GWAS) identified the Ydsphk1 gene as closely associated with the resistance of yellow drum (Nibea albiflora) to Vibrio harveyi. Structural prediction showed that YDSPHK1 contains a typical diacylglycerol kinase catalytic (DAGKc) domain (154–291 aa). By constructing and transfecting Ydsphk1 expression plasmids into yellow drum kidney cells, we found that YDSPHK1 is localized in the cytoplasm. Subsequent RNA-Seq analysis of an overexpression plasmid identified 25 differentially expressed genes (DEGs), including 13 upregulated and 12 downregulated. Notably, nsun5 and hsp90aa1 were significantly upregulated, while Nfkbia and hmox1 were downregulated. Promoter analysis indicated that the core regulatory regions of Ydsphk1 are located between −1931~−1679 bp and −419~+92 bp, with two predicted TFAP2A binding sites in the −419~+92 bp region. Further studies demonstrated that varying concentrations of TFAP2A significantly reduced Ydsphk1 promoter activity. These findings underscore the pivotal role of Ydsphk1 in regulating immune responses in yellow drum, particularly through its impact on key immune-related genes and pathways such as NF-κB signaling and ferroptosis. The identification of Ydsphk1 as a mediator of immune regulation provides valuable insights into the molecular mechanisms of immune defense and highlights its potential as a target for enhancing pathogen resistance in aquaculture practices. This study lays a strong foundation for future research aimed at developing innovative strategies for disease management in aquaculture species.
EX-CODE: A Robust and Explainable Model to Detect AI-Generated Code
Authors: Bulla Luana , Midolo Alessandro , Mongiovì Misael , Tramontana Emiliano
Published: December 2024

Distinguishing whether some code portions were implemented by humans or generated by a tool based on artificial intelligence has become hard. However, such a classification would be important as it could point developers towards some further validation for the produced code. Additionally, it holds significant importance in security, legal contexts, and educational settings, where upholding academic integrity is of utmost importance. We present EX-CODE, a novel and explainable model that leverages the probability of the occurrence of some tokens, within a code snippet, estimated according to a language model, to distinguish human-written from AI-generated code. EX-CODE has been evaluated on a heterogeneous real-world dataset and stands out for its ability to provide human-understandable explanations of its outcomes. It achieves this by uncovering the features that for a snippet of code make it classified as human-written code (or AI-generated code).
The Treatment of Patients with Early-Stage Non-Small Cell Lung Cancer Who Are Not Candidates or Decline Surgical Resection: The Role of Radiation and Image-Guided Thermal Ablation
Authors: Buchberger David S. , Khurana Rishabh , Bolen Michael , Videtic Gregory M. M.
Published: December 2024

The standard of care for early-stage NSCLC has historically been surgical resection. Given the association of lung cancer with smoking, a large number of early-stage patients also have active smoking-related medical comorbidities such as COPD precluding surgery. The current approach for treating such inoperable patients is frequently considered to be stereotactic body radiation therapy (SBRT). SBRT (also known as stereotactic ablative radiation therapy or SABR) is a curative modality that precisely delivers very high dose radiation in few (typically <5) sessions. That said, because of their minimal invasiveness and repeatable nature, image-guided thermal ablation therapies such as radiofrequency ablation (RFA), microwave ablation (MWA), and cryoablation (CA) have also been used to treat early-stage lung tumors. For those patients deemed to have “high operative risk” (i.e., those who cannot tolerate lobectomy, but are candidates for sublobar resection), the appropriateness of potential alternatives [e.g., SBRT; ablation] to surgery is an active area of investigation. In the absence of completed randomized phase III trials, the approach to comparing outcomes between surgery, SBRT, or ablative therapies by their efficacy or equivalence is complex. An overview of the role of SBRT and other non-surgical modalities in the management of early-stage lung cancer is the subject of the present review.
Distal Triceps Tendon Rupture—First Retrospective Study in Central Europe
Authors: Sekáč Jaroslav , Šagát Peter , Bartík Peter , Kilián Miroslav , Tohănean Dragoş Ioan , Perez Jason , Vasilcova Veronika , Durdík Štefan
Published: December 2024

Background: This retrospective study is the only one in the last 10 years from central Europe and provides a current picture of prevalence, new diagnostic modalities, new methods of surgical treatment, and also offers new insights into post-operative care. Triceps tendon rupture is the least reported among all the tendon injuries in the literature. In general, effective treatments for tendon injuries are lacking because the understanding of tendon biology lags behind that of the other components of the musculoskeletal system. Tendon tissue has a low number of cells and growth hormones and thus a lack of natural healing ability. Understanding the links between the mechanical and biological parameters involved in tendon development, homeostasis, and repair is a prerequisite for the identification of effective treatments for chronic and acute tendon injuries. Methods: The authors statistically evaluated the set of patients with this diagnosis in the largest University Hospital in Slovakia over the last 10 years. Results: Between 2014 and 2023, 23 patients with distal triceps tendon ruptures (DTTR) were treated at University Hospital. In some years not a single patient with this diagnosis underwent surgery, reinforcing the idea that DTTR may be either rare or underdiagnosed. The incidence in our region is 0.46 cases per 100,000 inhabitants. The average age of patients was 57.7 years, with a male predominance of 90%. Less than half of the patients (43.5%) underwent surgical intervention, and the median time from injury to surgery was less than 10 days. This rapid timeline indicates a high standard of medical care, given the semi-elective nature of the surgery and the need for MRI (Magnetic Resonance Imaging) confirmation of tendon rupture exceeding 50% of the fibers before proceeding with surgery. The three standard surgical techniques were employed in approximately equal proportions. Conclusions: This study suggests that none of the methods is currently preferred, and that the choice of the technique was largely determined by perioperative findings and the surgeon’s discretion. Post-operative complications were minimal, with only one patient experiencing any issues after surgery.
Efficacy of Transcranial Direct Current Stimulation (tDCS) on Neuropsychiatric Symptoms in Multiple Sclerosis (MS)—A Review and Insight into Possible Mechanisms of Action
Authors: Chmiel James , Stępień-Słodkowska Marta
Published: December 2024

Introduction: Neuropsychiatric symptoms such as depression and anxiety are a significant burden on patients with multiple sclerosis (MS). Their pathophysiology is complex and yet to be fully understood. There is an urgent need for non-invasive treatments that directly target the brain and help patients with MS. One such possible treatment is transcranial direct current stimulation (tDCS), a popular and effective non-invasive brain stimulation technique. Methods: This mechanistic review explores the efficacy of tDCS in treating depression and anxiety in MS while focusing on the underlying mechanisms of action. Understanding these mechanisms is crucial, as neuropsychiatric symptoms in MS arise from complex neuroinflammatory and neurodegenerative processes. This review offers insights that may direct more focused and efficient therapeutic approaches by investigating the ways in which tDCS affects inflammation, brain plasticity, and neural connections. Searches were conducted using the PubMed/Medline, ResearchGate, Cochrane, and Google Scholar databases. Results: The literature search yielded 11 studies to be included in this review, with a total of 175 patients participating in the included studies. In most studies, tDCS did not significantly reduce depression or anxiety scores as the studied patients did not have elevated scores indicating depression and anxiety. In the few studies where the patients had scores indicating mild/moderate dysfunction, tDCS was more effective. The risk of bias in the included studies was assessed as moderate. Despite the null or near-null results, tDCS may still prove to be an effective treatment option for depression and anxiety in MS, because tDCS produces a neurobiological effect on the brain and nervous system. To facilitate further work, several possible mechanisms of action of tDCS have been reported, such as the modulation of the frontal–midline theta, reductions in neuroinflammation, the modulation of the HPA axis, and cerebral blood flow regulation. Conclusions: Although tDCS did not overall demonstrate positive effects in reducing depression and anxiety in the studied MS patients, the role of tDCS in this area should not be underestimated. Evidence from other studies indicates the effectiveness of tDCS in reducing depression and anxiety, but the studies included in this review did not include patients with sufficient depression or anxiety. Future studies are needed to confirm the effectiveness of tDCS in neuropsychiatric dysfunctions in MS.
The Effect of Clinical Exercise Training on Plantar Pressure, the Subtalar Joint, and the Gait Cycle in Pregnant Women: Randomized Clinical Trial
Authors: Kayalı Vatansever Ayşe , Şenışık Seçkin , Bayraktar Dilek , Demir Mehmet , Akercan Fuat
Published: December 2024

Background/Objectives: This study aims to examine the effects of clinical exercise training on foot plantar pressure, the subtalar joint, and the gait cycle during pregnancy. Methods: The study was planned as a randomized, controlled, and single-blind study. Participants’ demographic information, obstetric evaluation, physical activity level, fall history, and pain evaluation were recorded. Foot plantar pressure, the subtalar joint, and the gait cycle were measured through pedobarography at Gait Laboratory. The control group was recommended walking. Clinical exercise training was given to the study group 2 days a week for eight weeks. Evaluations were made on day 0 and the day corresponding to the end of week 8. Results: The study was completed with 50 people in the study group (age: 29.7 ± 3.8 years) and 51 in the control group (age: 29.1 ± 6.1 years). As a result of the parametric and non-parametric tests performed before and after the exercise, it was observed that there was a statistically significant difference between the two groups in weight, BMI, pain score, static plantar pressure, dynamic plantar pressure, subtalar joint flexibility, duration of the walking period, and multistep walking speed (p < 0.01). The two groups had a significant difference only in the dominant midfoot plantar pressure (p > 0.05). Conclusions: According to our research, weight control and pain relief are observed in women who engage in clinical exercise in the second trimester of pregnancy; plantar pressure and subtalar joint flexibility are preserved, the walking period does not increase, and the multistep walking speed can be maintained after eight weeks.
Shear Wave Dispersion Elastography in ALD and MASLD: Comparative Pathophysiology and Clinical Potential—A Narrative Review
Authors: Dionisi Tommaso , Galasso Linda , Antuofermo Luigiandrea , Mancarella Francesco Antonio , Esposto Giorgio , Mignini Irene , Ainora Maria Elena , Gasbarrini Antonio , Addolorato Giovanni , Zocco Maria Assunta
Published: December 2024

Alcohol-related liver disease (ALD) is a major cause of global morbidity and mortality, progressing from steatosis to cirrhosis and hepatocellular carcinoma. While liver biopsy remains the gold standard for identifying liver disease, non-invasive methods like shear wave dispersion (SWD) elastography offer promising alternatives. This scoping review evaluates SWD’s potential in the study of ALD, comparing it to metabolic dysfunction-associated steatotic liver disease (MASLD). SWD measures changes in shear wave speed in relation to liver viscosity and necroinflammation. Studies in MASLD suggest that SWD effectively correlates with fibrosis and inflammation stages, but its application in ALD remains underexplored. Both ALD and MASLD show similar inflammatory and fibrotic pathways, despite having different etiologies and histological features. This review emphasizes the necessity to identify ALD-specific SWD reference values and verify SWD’s ability to improve diagnosis and disease progression. Prospective studies comparing SWD findings with histological benchmarks in ALD are essential for establishing its clinical utility. Incorporating SWD into clinical practice could revolutionize the non-invasive evaluation of ALD, offering a safer, cost-effective, and repeatable diagnostic tool.
The Role of Mirror Therapy in the Rehabilitation of the Upper Limb’s Motor Deficits After Stroke: Narrative Review
Authors: Ventoulis Ioannis , Gkouma Kyriaki-Rafaela , Ventouli Soultana , Polyzogopoulou Effie
Published: December 2024

Stroke is one of the leading causes of death and disability worldwide and poses a tremendous socioeconomic burden upon individuals, countries and healthcare systems. It causes debilitating symptoms and thus interferes with many aspects of the patient’s life, including physical functioning, cognition, emotional status, activities of daily living, social reintegration and quality of life. Post-stroke patients frequently experience functional motor disabilities of the upper limb, which restrict autonomy and self-efficacy and cause limitations in engagement with activities and social participation, as well as difficulties in performing important occupations. It is therefore not surprising that motor impairment or loss of motor function of the upper limb is one of the most devastating sequelae of stroke. On these grounds, achieving optimal functioning of the upper limb after stroke remains a fundamental goal of stroke rehabilitation. Mirror therapy (MT) represents one of the several rehabilitation techniques used for restoring the upper limb’s motor function after a stroke. However, conflicting results about the role of MT in the rehabilitation of the upper limb’s motor deficits have been reported in the literature. Accordingly, the aim of this narrative review is to summarize existing evidence regarding the effects of MT on the upper limb’s motor function in post-stroke patients and to further explore its role when applied in different phases of stroke.
Comparison of the Accuracy, Completeness, Reproducibility, and Consistency of Different AI Chatbots in Providing Nutritional Advice: An Exploratory Study
Authors: Ponzo Valentina , Rosato Rosalba , Scigliano Maria Carmine , Onida Martina , Cossai Simona , De Vecchi Morena , Devecchi Andrea , Goitre Ilaria , Favaro Enrica , Merlo Fabio Dario , Sergi Domenico , Bo Simona
Published: December 2024

Background: The use of artificial intelligence (AI) chatbots for obtaining healthcare advice is greatly increased in the general population. This study assessed the performance of general-purpose AI chatbots in giving nutritional advice for patients with obesity with or without multiple comorbidities. Methods: The case of a 35-year-old male with obesity without comorbidities (Case 1), and the case of a 65-year-old female with obesity, type 2 diabetes mellitus, sarcopenia, and chronic kidney disease (Case 2) were submitted to 10 different AI chatbots on three consecutive days. Accuracy (the ability to provide advice aligned with guidelines), completeness, and reproducibility (replicability of the information over the three days) of the chatbots’ responses were evaluated by three registered dietitians. Nutritional consistency was evaluated by comparing the nutrient content provided by the chatbots with values calculated by dietitians. Results: Case 1: ChatGPT 3.5 demonstrated the highest accuracy rate (67.2%) and Copilot the lowest (21.1%). ChatGPT 3.5 and ChatGPT 4.0 achieved the highest completeness (both 87.3%), whereas Gemini and Copilot recorded the lowest scores (55.6%, 42.9%, respectively). Reproducibility was highest for Chatsonic (86.1%) and lowest for ChatGPT 4.0 (50%) and ChatGPT 3.5 (52.8%). Case 2: Overall accuracy was low, with no chatbot achieving 50% accuracy. Completeness was highest for ChatGPT 4.0 and Claude (both 77.8%), and lowest for Copilot (23.3%). ChatGPT 4.0 and Pi Ai showed the lowest reproducibility. Major inconsistencies regarded the amount of protein recommended by most chatbots, which suggested simultaneously to both reduce and increase protein intake. Conclusions: General-purpose AI chatbots exhibited limited accuracy, reproducibility, and consistency in giving dietary advice in complex clinical scenarios and cannot replace the work of an expert dietitian.
Numerical Simulation on the Frequency Response of 3-D Reef–Seawater–Seabed Coupling System Under Seismic Excitation
Authors: Yan Liwen , Guo Xingwei , Zhang Xunhua , Qi Jianghao
Published: December 2024

The seismic safety evaluation of artificial reef islands is of great significance for ensuring their long-term stable operation and the safety of residents’ lives. However, due to an insufficient understanding of coral reefs’ basic characteristics, current research on coral reef seismic stability neglects the influence of pore water pressure and abnormal reef layers formed during geological evolution. To further study the impact of earthquakes on coral reefs in the South China Sea, this paper takes Meiji Reef as the research object, establishes a 3-D model containing a saturated coral reef–seawater–seabed coupling system, and considers the influence of abnormally high-porosity weathered layers to study the seismic response of the coupling system in the frequency domain. The results show that ignoring the influence of pore water pressure will underestimate the impact of earthquakes on coral reefs. The seismic waves with a frequency of 4.1 Hz in the horizontal direction have a significant impact on the reef, and the side parallel to the direction of wave propagation is more affected, while the side perpendicular to the direction of wave propagation is less affected. The reef flat near the seawater side is less affected by earthquakes, while that on the lagoon side is more affected. Highly porous, weathered layers increase the seismic impact on reef flats.
A 3D Dual-Particle Imaging Algorithm for Multiple Imagers
Authors: Garg Dhruv , Lopez Ricardo , Pakari Oskari , Clarke Shaun D. , Pozzi Sara A.
Published: December 2024

The ability to localize and image radiation sources has found use in various applications for nuclear nonproliferation practices, specifically in treaty verification, nuclear safeguards, and homeland security. Technologies that are capable of angular radiation imaging have been prevalent for years and, recently, 3D imaging technologies making use of emerging media like mixed reality have been rapidly developing and gaining popularity. Modern imaging techniques typically use a Compton camera to record coincident events and reconstruct the incident directional information of a gamma ray-emitting radiation source. However, Compton cameras are limited as they cannot obtain accurate source depth information when used for simple back projection imaging. Neutron scatter cameras are a complementary imaging technique that use double elastic scatters but also have their own limitations. This work presents a framework for multiple scatter-based particle imagers to construct 3D images and to localize a radiation source using gamma rays or fast neutrons. Specifically, localization is achieved by accounting for the position of the imagers. The imaging algorithm was validated using experimental data, measuring a 252Cf source. A three-dimensional representation of the imaging data provides a more intuitive and informative depiction of source positions and can aid in scenarios with complex environmental geometries such as when sources are in containers.
Application of Metal Oxide Nanoparticles in Different Carcinomas
Authors: Rani Nutan , Khan Yousuf , Yadav Sapna , Saini Kalawati , Maity Dipak
Published: December 2024

Metal oxide nanoparticles (MONPs) have recently attracted much attention from researchers due to their use in cancer chemotherapy, targeted drug delivery, and diagnosis/MRI imaging. Various studies have demonstrated that different metal oxide NPs show cytotoxic effects by inducing apoptosis in cancerous cells and do not have any toxic impact on normal cells. The mechanism of cytotoxicity is shown through reactive oxygen species (ROS) generated by (MONPs) in the cancerous cell. In vitro and in vivo studies reveal that in some cases metal oxide NPs are used alone and somewhere these NPs are used in combination with other therapies such as photodynamic therapy and with anticancer nanomedicines as drug carriers or drug conjugates. The phenomenon of enhanced permeability and retention (EPR) effect has been the basis of targeted drug delivery to cancerous tumors. Finally, we also provide a simple and comparative analysis of the major apoptosis pathways proposed to increase beginner understanding of anti-cancer nanomaterials. Herein, we have reviewed the most important antitumor results obtained with different metal oxide nanoparticles such as ZnO, Fe2O3/Fe3O4, CuO/Cu2O, TiO2, CeO2, and HfO2, respectively. These NPs can be applied to treat cancer by either passive or active processes. A passive process uses the enhanced permeability and retention (EPR) effect. Superparamagnetic iron oxide nanoparticles (SPIONs), due to their unique magnetic and physiochemical properties have been used in magnetic fluid hyperthermia (MFH) and magnetic resonance imaging (MRI) in vitro as well as in vivo. Now, the research has reached the stage of clinical trials for the treatment of various types of cancer. ZnO NPs have been used very vastly in cytotoxic as well as in targeted drug delivery. These NPs are also used for loading anticancer drugs such as doxorubicin. Herein, in this review, we have examined current advances in utilizing MONPs and their analogs as cancer therapeutic, diagnostic, and drug-delivery agents.
Personalized Recommendation in a Retail Platform Under the Hybrid Selling Mode
Authors: Wang Wei , Han Xinyu , Ma Yuqing , Li Gang
Published: December 2024

Retail platforms have widely implemented recommender systems to provide personalized recommendations to consumers, influencing sales significantly. However, under the hybrid selling mode where platforms offer both their products and third-party sellers’ products, the profitability of a recommender system and the optimal allocation of recommendations become critical considerations. This paper introduces a game-theoretic model to investigate these issues and unveil how a recommender system and its characteristics influence prices and profits. A key finding is that the recommender system increases prices and profits only if the commission rate is high and the system is profit-oriented or inaccurate. Surprisingly, higher recommendation accuracy does not always translate into higher profits; it is advantageous only in a consumer-oriented system. Moreover, the retail platform tends to allocate more recommendations to its own product than to the third-party seller’s product, a strategy known as self-preferencing. This strategy gives the platform a competitive edge and boosts its profit compared to the third-party seller. Furthermore, the degree of self-preferencing varies with the accuracy and orientation of the recommendation system. Specifically, in a consumer-oriented system, self-preferencing increases with accuracy, while in a profit-oriented system, it decreases with accuracy.
Early Study on Visible (Vis) and Short-Wave Infrared (SWIR) Spectroscopy for Assessing Water Content in Olive Fruits: Towards Sustainable Land and Agricultural Practices
Authors: Bonifazi Giuseppe , Gasbarrone Riccardo , Gattabria Davide , Lendaro Eugenio , Mosca Luciana , Mattioli Roberto , Serranti Silvia
Published: December 2024

Accurate and rapid assessment of the water content in olive fruits is critical for enhancing the efficiency and sustainability of olive oil production. This study investigates the application of visible and short-wave infrared (Vis-SWIR) spectroscopy as a non-invasive method to directly measure the water content in intact olive fruits before milling, also affecting eco-friendly farming practices. Partial least squares (PLS) regression models for the olive fruit weight, weight loss, and water content were developed while using the dehydration process in a drying oven as the reference analysis. The models demonstrated strong predictive performance, with the PLS model for the olive fruit weight achieving a coefficient of determination in cross-validation (R2CV) of 0.78 and a root mean squared error (RMSECV) of 0.6 g. Additionally, for olive fruit weight loss, a R2CV of 0.96 with an RMSECV of 4.5% was achieved. Meanwhile, for the olive fruit water content, an R2CV of 0.94 with an RMSECV of 0.245 mL was obtained. The PLS regression model set up to predict the water content for intact olive fruits showed promise, as evidenced by its fit, RMSE in prediction, and residual prediction deviation (RPD) values (R2P = 0.80, RMSEP = 0.556 mL, and RPD = 2.247). The obtained results indicate that portable Vis-SWIR spectrophotometers provide a rapid and efficient alternative to conventional drying and weighing methods, facilitating early detection of olive fruit quality. This technological approach not only enhances the financial returns for producers but also supports sustainable agricultural practices. The use of Vis-SWIR spectroscopy has broader potential applications in the olive industry, including quality control, monitoring the water status of olive orchards, and optimizing irrigation management, contributing to the sustainable management of land and agricultural resources.
Evaluating Agri-Environmental Indicators for Land Use Impact in Baltic Countries Using Multi-Criteria Decision-Making and Eurostat Data
Authors: Štreimikienė Dalia , Bathaei Ahmad , Streimikis Justas
Published: December 2024

The present research assesses the agri-environmental sustainability of the Baltic states, namely Lithuania, Latvia, and Estonia, while analyzing agricultural biodiversity, greenhouse gas emissions, land utilization, energy use, and water management. For the purpose of these evaluations and ranking, we employ the Technique for Order Preference by Similarity to Ideal Solution (TOPSIS), applied to a set of agri-environmental indicators (AES) collected from various sources, including Eurostat and similar databases. This knowledge is necessary to help policymakers or agricultural managers who are interested in developing more sustainable agriculture in the region. That is why, according to the findings, the highest AES value is attributed to Latvia, followed by Lithuania and Estonia. Conservation methods that were essential included High-Nature-Value farmland, bird populations in agricultural habitats, organic farming, and water. It emphasizes the use of Multi-Criteria Decision Making tools for combining both qualitative and quantitative data and form the general framework for sustainability assessment. Another goal of this research is to fill the gap in the literature regarding the lack of attention paid to agri-environmental sustainability in the Baltic area in general. This, therefore, suggests that assessing the strengths or weaknesses of these nation-states provides critical information that can inform the change of land management practices, nutrition practices in agriculture, and the ability of the natural world to adapt. The findings will be useful to governmental leaders and individuals involved in agriculture who need to find a balance between economic growth and conservation, as well as scholars working to improve the international measures for agri-environmental assessment.
New Insights into a Vanadium Precipitation Process by Hydrolysis Based on Manganese Salt Pretreatment
Authors: Liu Mengxia , Jiang Tao , Wen Jing , Fu Zibi , Yu Tangxia , Yang Guangdong , Xia Sanyuan , Xiao Hao
Published: December 2024

Vanadium precipitation is the key step in producing vanadium products from vanadium solution. The sustainable development of the vanadium industry requires new environmentally friendly processes for vanadium precipitation. In this study, NaVO3 solution was pretreated with manganese salt to preliminarily separate the vanadium and sodium components. The product of vanadium extraction by manganese salt was dissolved by acid to produce manganese vanadate solution. After vanadium precipitation by hydrolysis, manganese removal, and calcination, the target product V2O5 was obtained. Scanning electron microscopy (SEM), X-ray diffraction (XRD), inductively coupled plasma emission spectrometry (ICP-OES), and atomic absorption spectrometry (AAS) were used to perform the characterization and analyses. The results showed that vanadium and manganese have a strong binding ability. The rate of vanadium extraction by manganese salt reached 99.75%, and the product of vanadium extraction by manganese salt was Mn2V2O7, with a sodium content of only 0.089%, confirming the effective separation of vanadium and sodium. The acid dissolution rate of the vanadium extraction product reached 99.95%, and the rate of vanadium precipitation by hydrolysis reached 97.87%. After manganese removal and calcination, the purity of the V2O5 product reached 98.92%. In addition, the recyclability of manganese sulfate and ammonium sulfate was analyzed. The process reduced the production of ammonia–nitrogen wastewater, laying a foundation for researching new technologies for extracting vanadium from vanadium slag.
Nondestructive Monitoring of Textile-Reinforced Cementitious Composites Subjected to Freeze–Thaw Cycles
Authors: Ospitia Nicolas , Pourkazemi Ali , Tsangouri Eleni , Tayeh Thaer , Stiens Johan H. , Aggelis Dimitrios G.
Published: December 2024

Cementitious materials are susceptible to damage not only from mechanical loading, but also from environmental (physical, chemical, and biological) factors. For Textile-Reinforced Cementitious (TRC) composites, durability poses a significant challenge, and a reliable method to assess long-term performance is still lacking. Among various durability attacks, freeze–thaw can induce internal cracking within the cementitious matrix, and weaken the textile–matrix bond. Such cracks result from hydraulic, osmotic, and crystallization pressure arising from the thermal cycles, leading to a reduction in the stiffness in the TRC composites. Early detection of freeze–thaw deterioration can significantly reduce the cost of repair, which is only possible through periodic, full-field monitoring of the composite. Full-field monitoring provides a comprehensive view of the damage distribution, offering valuable insights into the causes and progression of damage. The crack location, size, and pattern give more information than that offered by single-point measurement. While visual inspections are commonly employed for crack assessment, they are often time-consuming. Technological advances now enable crack pattern classification based on high-quality surface images; however, these methods only provide information limited to the surface. Elastic wave-based non-destructive testing (NDT) methods are highly sensitive to the material’s mechanical properties, and therefore are widely used for damage monitoring. On the other hand, electromagnetic wave-based NDTs offer the advantage of fast, non-contact measurements. Micro- and millimeter wave frequencies offer a balance of high resolution and wave penetration, although they have not yet been sufficiently explored for detecting damage in cementitious composites. In this study, TRC specimens were subjected to up to 150 freeze–thaw cycles and monitored using a combination of active elastic and electromagnetic wave-based NDT mapping methods. For this purpose, transmission measurements were conducted at multiple points, with ultrasonic pulse velocity (UPV) employed as a benchmark and, for the first time, millimeter wave (MMW) spectrometry applied. This multi-modal mapping approach enabled the tracking of damage progression, and the identification of degraded zones.