bluuebunny/crossref_metadata_2025 huggingface dataset:
Carbon trading-based layered operation optimization of the electric–thermal multi-energy-flow coupling system with photothermal power stations
Authors: Hu Changbin , Cai Xiaoqin , Zhao Xinyu , Luo Shanna , Lu Heng , Li Xuecheng
Published: October 2023

With the increasing development of low-carbon economy, the coupling degree of electric thermal gas system is deepening day by day. The operation mode of traditional energy supply system using electric heating separation mode and hierarchical dispatching of transmission and distribution network has been difficult to mine the whole network resources and realize the global optimal operation strategy. In order to increase the consumption of new energy and solve the multi-energy current coupling system, this paper puts forward a layered optimization operation strategy for the electric-thermal multi-energy current coupling system with Concentrated solar power (CSP) plant and carbon trading mechanism. The upper layer is the solution layer of multi-energy coupled flow system. In order to solve the matrix values of complex multi-energy coupled flow system, Newton's method, improved Newton’s method and improved second-order cone collaborative solution methods are proposed. The lower layer is the multi-energy flow optimization layer. According to the system solution values obtained from the upper layer, the lower layer optimization aims at economic minimization of the total user cost. The carbon trading mechanism model is introduced to optimize the time sequence output of the electric thermal unit under different scenarios, and the mixed integer linear programming method is adopted. Finally, the validity is verified by a simulation example. The results show that this method can optimize the operation of the system, improve the accuracy and rapidity of the system, and “carbon trading and CSP power station” method can better constrain the carbon emission of the multi-energy flow coupling system, reduce the energy pressure of the device, and improve the economy of the total cost of the user.If this method is adopted, it can be used for reference in the future energy management and system decision of multi-energy flow coupling system.
ODAD1 variants resulting from splice-site mutations retain partial function and cause primary ciliary dyskinesia with outer dynein arm defects
Authors: Zhou Nannan , Liang Weilin , Zhang Yanzhu , Quan Guoli , Li Ting , Huang Siqing , Huo Yating , Cui Haiyan , Cheng Yuanxiong
Published: October 2023

Primary ciliary dyskinesia (PCD) is a genetically heterogeneous disorder caused by defects in motile ciliary function and/or structure. Outer dynein arm docking complex subunit 1 (ODAD1) is an important component of the outer dynein arm docking complex (ODA-DC). To date, 13 likely pathogenic mutations of ODAD1 have been reported. However, the pathogenesis of ODAD1 mutations remains elusive. To investigate the pathogenesis of splice-site mutations in ODAD1 discovered in this study and those reported previously, molecular and functional analyses were performed. Whole-exome sequencing revealed a compound mutation in ODAD1 (c.71-2A>C; c.598-2A>C) in a patient with PCD, with c.598-2A>C being a novel mutation that resulted in two mutant transcripts. The compound mutation in ODAD1 (c.71-2A>C; c.598-2A>C) led to aberrant splicing that resulted in the absence of the wild-type ODAD1 and defects of the outer dynein arm in ciliary axonemes, causing a decrease in ciliary beat frequency. Furthermore, we demonstrated that the truncated proteins resulting from splice-site mutations in ODAD1 could retain partial function and inhibit the interaction between wild-type ODAD1 and ODAD3. The results of this study expand the mutational and clinical spectrum of PCD, provide more evidence for genetic counseling, and offer new insights into gene-based therapeutic strategies for PCD.
Further characterization of Shigella-specific (memory) B cells induced in healthy volunteer recipients of SF2a-TT15, a Shigella flexneri 2a synthetic glycan-based vaccine candidate
Authors: Toapanta Franklin R. , Hu Jingping , Meron-Sudai Shiri , Mulard Laurence A. , Phalipon Armelle , Cohen Dani , Sztein Marcelo B.
Published: October 2023

Shigellosis is common worldwide, and it causes significant morbidity and mortality mainly in young children in low- and middle- income countries. To date, there are not broadly available licensed Shigella vaccines. A novel type of conjugate vaccine candidate, SF2a-TT15, was developed against S. flexneri serotype 2a (SF2a). SF2a-TT15 is composed of a synthetic 15mer oligosaccharide, designed to act as a functional mimic of the SF2a O-antigen and covalently linked to tetanus toxoid (TT). SF2a-TT15 was recently shown to be safe and immunogenic in a Phase 1 clinical trial, inducing specific memory B cells and sustained antibody response up to three years after the last injection. In this manuscript, we advance the study of B cell responses to parenteral administration of SF2a-TT15 to identify SF2a LPS-specific B cells (SF2a+ B cells) using fluorescently labeled bacteria. SF2a+ B cells were identified mainly within class-switched B cells (SwB cells) in volunteers vaccinated with SF2a-TT15 adjuvanted or not with aluminium hydroxide (alum), but not in placebo recipients. These cells expressed high levels of CXCR3 and low levels of CD21 suggesting an activated phenotype likely to represent the recently described effector memory B cells. IgG SF2a+ SwB cells were more abundant than IgA SF2a + SwB cells. SF2a+ B cells were also identified in polyclonally stimulated B cells (antibody secreting cells (ASC)-transformed). SF2a+ ASC-SwB cells largely maintained the activated phenotype (CXCR3 high, CD21 low). They expressed high levels of CD71 and integrin α4β7, suggesting a high proliferation rate and ability to migrate to gut associated lymphoid tissues. Finally, ELISpot analysis showed that ASC produced anti-SF2a LPS IgG and IgA antibodies. In summary, this methodology confirms the ability of SF2a-TT15 to induce long-lived memory B cells, initially identified by ELISpots, which remain identifiable in blood up to 140 days following vaccination. Our findings expand and complement the memory B cell data previously reported in the Phase 1 trial and provide detailed information on the immunophenotypic characteristics of these cells. Moreover, this methodology opens the door to future studies at the single-cell level to better characterize the development of B cell immunity to Shigella.
The role of ultrasound in predicting non-invasive ventilation outcomes: a systematic review
Authors: Kheir Matthew , Dong Vincent , Roselli Victoria , Mina Bushra
Published: October 2023

Purpose

To systematically review and compare ultrasonographic methods and their utility in predicting non-invasive ventilation (NIV) outcomes.

Methods

A systematic review was performed using the PubMed, Medline, Embase, and Cochrane databases from January 2015 to March 2023. The search terms included the following: ultrasound, diaphragm, lung, prediction, non-invasive, ventilation, and outcomes. The inclusion criteria were prospective cohort studies on adult patients requiring non-invasive ventilation in the emergency department or inpatient setting.

Results

Fifteen studies were analyzed, which comprised of 1,307 patients (n = 942 for lung ultrasound score studies; n = 365 patients for diaphragm dysfunction studies). Lung ultrasound scores (LUS) greater than 18 were associated with NIV failure with a sensitivity 62–90.5% and specificity 60–91.9%. Similarly, a diaphragm thickening fraction (DTF) of less than 20% was also associated with NIV failure with a sensitivity 80–84.6% and specificity 76.3–91.5%.

Conclusion

Predicting NIV failure can be difficult by routine initial clinical impression and diagnostic work up. This systematic review emphasizes the importance of using lung and diaphragm ultrasound, in particular the lung ultrasound score and diaphragm thickening fraction respectively, to accurately predict NIV failure, including the need for ICU-level of care, requiring invasive mechanical ventilation, and resulting in higher rates of mortality.
Genetic evidence for the interaction between Bacillus anthracis-encoded phage receptors and their cognate phage-encoded receptor binding proteins
Authors: Forrest Samantha , Ton Sarah , Sholes Samantha L. , Harrison Sarah , Plaut Roger D. , Verratti Kathleen , Wittekind Michael , Ettehadieh Elham , Necciai Bryan , Sozhamannan Shanmuga , Grady Sarah L.
Published: October 2023

Bacteriophages such as γ and AP50c have been shown to infect strains of Bacillus anthracis with high specificity, and this feature has been exploited in the development of bacterial detection assays. To better understand the emergence of phage resistance, and thus the potential failure of such assays, it is important to identify the host and phage receptors necessary for attachment and entry. Using genetic approaches, the bacterial receptors of AP50c and γ have been identified as sap and GamR, respectively. A second AP50c-like phage, Wip1, also appears to use sap as a receptor. In parallel with this work, the cognate phage-encoded receptor binding proteins (RBPs) have also been identified (Gp14 for γ, P28 for AP50c, and P23 for Wip1); however, the strength of evidence supporting these protein–protein interactions varies, necessitating additional investigation. Here, we present genetic evidence further supporting the interaction between sap and the RBPs of AP50c and Wip1 using fluorescently tagged proteins and a panel of B. anthracis mutants. These results showed that the deletion of the sap gene, as well as the deletion of csaB, whose encoded protein anchors sap to the bacterial S-layer, resulted in the loss of RBP binding. Binding could then be rescued by expressing these genes in trans. We also found that the RBP of the γ-like prophage λBa03 relied on csaB activity for binding, possibly by a different mechanism. RBPλBa03 binding to B. anthracis cells was also unique in that it was not ablated by heat inactivation of vegetative cells, suggesting that its receptor is still functional following incubation at 98°C. These results extend our understanding of the diverse attachment and entry strategies used by B. anthracis phages, enabling future assay development.
Soil salinity is the main factor influencing the soil bacterial community assembly process under long-term drip irrigation in Xinjiang, China
Authors: Li Dongwei , Qiu Husen , Tian Guangli , Zhao Yulong , Zhou Xinguo , He Shuai
Published: October 2023

Identifying the potential factors associated with the impact of long-term drip irrigation (DI) on soil ecosystems is essential for responding to the environmental changes induced by extensive application of DI technology in arid regions. Herein, we examined the effects of the length of time that DI lasts in years (NDI) on soil bacterial diversity as well as the soil bacterial community assembly process and the factors influencing it. The results showed that long-term DI substantially reduced soil salinity and increased soil bacterial diversity while affecting the soil bacterial community structure distinctly. Null model results showed that the soil bacterial community assembly transitioned from stochastic processes to deterministic processes, as NDIincreased. Homogeneous selection, a deterministic process, emerged as the dominant process when NDIexceeded 15 years. Both random forest and structural equation models showed that soil salinity was the primary factor affecting the bacterial community assembly process. In summary, this study suggested that soil bacteria respond differently to long-term DI and depends on the NDI, influencing the soil bacterial community assembly process under long-term DI.
High-throughput quantitation of amino acids and acylcarnitine in cerebrospinal fluid: identification of PCNSL biomarkers and potential metabolic messengers
Authors: Ma Jingjing , Chen Kun , Ding Yun , Li Xiao , Tang Qiming , Jin Bo , Luo Ruben Y. , Thyparambil Sheeno , Han Zhi , Chou C. James , Zhou Ashlee , Schilling James , Lin Zhiguang , Ma Yan , Li Qing , Zhang Mengxue , Sylvester Karl G. , Nagpal Seema , McElhinney Doff B. , Ling Xuefeng B. , Chen Bobin
Published: October 2023

Background: Due to the poor prognosis and rising occurrence, there is a crucial need to improve the diagnosis of Primary Central Nervous System Lymphoma (PCNSL), which is a rare type of non-Hodgkin’s lymphoma. This study utilized targeted metabolomics of cerebrospinal fluid (CSF) to identify biomarker panels for the improved diagnosis or differential diagnosis of primary central nervous system lymphoma (PCNSL).

Methods: In this study, a cohort of 68 individuals, including patients with primary central nervous system lymphoma (PCNSL), non-malignant disease controls, and patients with other brain tumors, was recruited. Their cerebrospinal fluid samples were analyzed using the Ultra-high performance liquid chromatography - tandem mass spectrometer (UHPLC-MS/MS) technique for targeted metabolomics analysis. Multivariate statistical analysis and logistic regression modeling were employed to identify biomarkers for both diagnosis (Dx) and differential diagnosis (Diff) purposes. The Dx and Diff models were further validated using a separate cohort of 34 subjects through logistic regression modeling.

Results: A targeted analysis of 45 metabolites was conducted using UHPLC-MS/MS on cerebrospinal fluid (CSF) samples from a cohort of 68 individuals, including PCNSL patients, non-malignant disease controls, and patients with other brain tumors. Five metabolic features were identified as biomarkers for PCNSL diagnosis, while nine metabolic features were found to be biomarkers for differential diagnosis. Logistic regression modeling was employed to validate the Dx and Diff models using an independent cohort of 34 subjects. The logistic model demonstrated excellent performance, with an AUC of 0.83 for PCNSL vs. non-malignant disease controls and 0.86 for PCNSL vs. other brain tumor patients.

Conclusion: Our study has successfully developed two logistic regression models utilizing metabolic markers in cerebrospinal fluid (CSF) for the diagnosis and differential diagnosis of PCNSL. These models provide valuable insights and hold promise for the future development of a non-invasive and reliable diagnostic tool for PCNSL.
Inhibitory neuron map of sevoflurane induced neurotoxicity model in young primates
Authors: Niu Yixuan , Cheng Yanyong , Miao Zhengjie , Xu Jinnan , Jiang Hong , Yan Jia
Published: October 2023

Introduction

Sevoflurane, one of the most commonly used anesthetic agents in children, may induce neuronal dysfunction and cognitive impairment. Exposure to sevoflurane might induce an imbalance between neural excitation and inhibition which could be a mechanism behind anesthesia-induced cognitive and affective dysfunctions. However, the underlying mechanisms remain unclear.

Methods

In this study, we used two rhesus macaques in the control group, and one rhesus macaques in the anesthesia group. We employed single-nucleus RNA sequencing (snRNA-seq) technology to explore alterations in distinct types of inhibitory neurons involved in the long-term cognitive impairment caused by sevoflurane in young macaques.

Results

Following sevoflurane treatment, an upregulation was observed in the SST+ inhibitory neuron in the LHX6+ neighborhood in the hippocampus of rhesus macaques. This alteration might impact brain development by influencing interneuron migration and maturation. Additionally, we proposed a novel classification of inhibitory neurons, defined by CNR1 and LHX6 applicable to both humans and macaques.

Discussion

Our study proposed a novel classification of inhibitory neurons defined by LHX6 and CNR1, relevant in macaques and humans. We also provide evidence that sevoflurane upregulated the SST+ inhibitory neuron in the LHX6+ neighborhood in the hippocampus of rhesus macaques, which may underlie the potential neurotoxic effects induced by general anesthetics. Our results also offer a more reliable approach for studying the structure and function of the human brain.
Association of concomitant continuous pain in trigeminal neuralgia with a narrow foramen ovale
Authors: Li Shuo , Liao Chenlong , Yang Xiaosheng , Zhang Wenchuan
Published: October 2023

Background

The pathogenesis of concomitant continuous pain remains unclear and is worthy of further study. In this clinical study, we aimed to explore the potential role of a narrow foramen ovale in the development of concomitant continuous pain.

Methods

A total of 108 patients with classical trigeminal neuralgia affecting the third branch of the trigeminal nerve and 46 healthy individuals were enrolled in this study. Three-dimensional reconstructed computerized tomography images of all participants were collected, and the morphometric features of the foramen ovale were examined by two investigators who were blinded to the clinical data of the patients.

Results

In this cohort, patients with concomitant continuous pain suffered from more sensory abnormalities (18.4% vs. 2.9%, p = 0.015) and responded more poorly to medication (74.3% vs. 91.9%, p = 0.018) than patients without concomitant continuous pain. While no significant differences regarding the mean length (5.02 mm vs. 5.36 mm, p > 0.05) and area (22.14 mm2 vs. 23.80 mm2, p > 0.05) were observed between patients with and without concomitant continuous pain, the mean width of the foramen ovale on the affected side in patients with concomitant continuous pain was significantly narrower than that in patients without concomitant continuous pain (2.01 mm vs. 2.48 mm, p = 0.003).

Conclusion

This neuroimaging and clinical study demonstrated that the development of concomitant continuous pain was caused by the compression of the trigeminal nerve owing to a narrow foramen ovale rather than responsible vessels in classical trigeminal neuralgia.
Association between tea drinking and disability levels in older Chinese adults: a longitudinal analysis
Authors: Ma Yinghui , Zhu Yuying , Hong Dandan , Zhao Haiyue , Li Lei
Published: October 2023

Objective

As the global population ages, disability among the elderly presents unprecedented challenges for healthcare systems. However, limited research has examined whether dietary interventions like tea consumption may alleviate and prevent disability in older adults. As an important dietary therapy, the health benefits of tea drinking have gained recognition across research disciplines. Therefore, this study aimed to investigate the association between tea drinking habits and disability levels in the elderly Chinese population.

Methods

Leveraging data from the 2008 to 2018 waves of the Chinese Longitudinal Healthy Longevity Survey, we disaggregated tea drinking frequency and activities of daily living (ADL) measures and deployed fixed-effect ordered logit models to examine the tea-disability association for the first time. We statistically adjusted for potential confounders and conducted stratified analyses to assess heterogeneity across subpopulations.

Results

Multivariable fixed-effect ordered logistic regression suggested tea drinking has protective effects against ADL disability. However, only daily tea drinking was associated with lower risks of basic activities of daily living (BADL) disability [odds ratio (OR) = 0.61; 95% confidence interval (CI), 0.41–0.92] and lower levels of instrumental activities of daily living (IADL) disability (OR = 0.78; 95% CI, 0.64–0.95). Stratified analyses indicated heterogeneous effects across age and income groups. Daily tea drinking protected against BADL (OR = 0.26 and OR = 0.28) and IADL disability (OR = 0.48 and OR = 0.45) for adults over 83 years old and high-income households, respectively.

Conclusion

We found that drinking tea almost daily was protective against disability in elderly people, warranting further research into optimal dosages. Future studies should utilize more rigorous causal inference methods and control for confounders.
Paraneoplastic dermatomyositis associated with urothelial cancer: report of a case and systematic review of the literature
Authors: Sabaté-Ortega Josep , Bujons-Buscarons Elisabet , Fina-Planas Clàudia , Vilanova-Anducas Núria , Vidal-Sarró Noemí , Sala-González Núria
Published: October 2023

Background

The idiopathic inflammatory myopathies (IIM) are a collection of autoimmune diseases that have a substantial impact on the entire body and include conditions such as dermatomyositis (DM), polymyositis (PM), sporadic inclusion body myositis, and immune-mediated necrotizing myopathy. These disorders are characterized by symptoms such as muscular weakness, pain, and dermal rash. This systematic review is intended to explore the potential link between bladder cancer and DM/PM.

Methods

We performed a comprehensive systematic search on PubMed and Scopus until August 2022 to identify relevant research studies. The studies that met our inclusion criteria focused on patients with urinary bladder cancer and dermatomyositis, and/or polymyositis.

Results

The patients’ median age was 65.5 years (47–79), with the majority being male (15, 39.47%). Bladder cancer manifested before PM/DM in 5 (13.15%) patients, while in the majority of cases occurred after the cancer diagnosis. The stage of cancer at the time of the initial PM/DM diagnosis were mostly locally (11/20, 50%).During the first presentation, the patients had a median creatine kinase level of 2227 U/L, ranging between 44 and 10471. In one case, anti-TIF-1γ antibodies were found to be present. Among the cases with reported medical history (20/38), treatment immediately improved DM symptoms in 16 patients(53.8%) and in 3 patients(15%), symptoms of DM resurfaced during the period after the operation. Death was reported in 14 (36.8%) patients.

Conclusion

In conclusion, our study provides knowledge and understanding for identifying specific risk factors in patients with the coexistence of bladder cancer and DM/PM and their management. During the initial and follow-up screening, age, gender, and the clinicopathological subgroup of myositis should be considered to ensure proper management of the condition.
Neuropsychiatric adverse drug reactions with oral tyrosine kinase inhibitors in metastatic colorectal cancer: an analysis from the FDA Adverse Event Reporting System
Authors: Barbieri Maria Antonietta , Russo Giulia , Sorbara Emanuela Elisa , Cicala Giuseppe , Franchina Tindara , Santarpia Mariacarmela , Speranza Desirèe , Spina Edoardo , Silvestris Nicola
Published: October 2023

Introduction

New oral tyrosine kinase inhibitors (TKIs) are approved for metastatic colorectal cancer (mCRC). The aim of this study was to assess the neuropsychiatric adverse drug reactions (ADRs) of these drugs reported in the FDA Adverse Event Reporting System (FAERS) database.

Methods

All reports with regorafenib (REG) and encorafenib (ENC) as the primary suspect, and reported in the FAERS between 2012 and 2022, were collected. A descriptive and disproportionality analyses were conducted.

Results

Out of 4,984 cases, 1,357 (30.2%) reported at least one neuropsychiatric ADR. New potential signals for REG included neuropathy peripheral (n = 265; reporting odds ratio, ROR = 19.48, 95% confidence interval, CI 95% = 17.52-22.47; information component, IC = 2.89, IC025-IC075 = 2.77-3.02), hyperesthesia (n = 18; ROR = 12.56, CI 95% = 7.90-19.96; IC = 2.25, IC025-IC075 = 1.79-2.72), taste disorder (n = 41; ROR = 9.91, CI 95% = 7.29-13.49; IC = 2.18, IC025-IC075 = 1.88-2.49), poor quality sleep (n = 18; ROR = 6.56, CI 95% = 4.13-10.42; IC = 1.74, IC025-IC075 = 1.27-2.20), altered state of consciousness (n = 15; ROR = 5.50, CI 95% = 3.31-9.14; IC = 1.57, IC025-IC075 = 1.06-2.07), depressed mood (n = 13; ROR = 1.85, CI 95% = 1.07-3.19; IC = 0.58, IC025-IC075 = 0.04-1.13) and insomnia (n = 63; ROR = 1.48, CI 95% = 1.15-1.89; IC = 0.38, IC025-IC075 = 0.13-0.63). For ENC comprised depressed mood (n = 4; ROR = 5.75, CI 95% = 2.15-15.39; IC = 1.74, IC025-IC075 = 0.76-2.73) and cognitive disorders (n = 3; ROR = 4.71, CI 95% = 1.51-14.66; IC = 1.54, IC025-IC075 = 0.41-2.68).

Discussion

This study identified new unknown potential neuropsychiatric ADRs. Further investigations are required to better define the neurotoxicity of TKIs in mCRC patients.
Targeting estrogen signaling and biosynthesis for aged skin repair
Authors: Zomer Helena D. , Cooke Paul S.
Published: October 2023

Non-healing skin wounds are disproportionally prevalent in older adults. Current treatments do not account for the particularities of aged skin and result in inadequate outcomes. Overall, healing chronic wounds in the elderly remains a major unmet clinical need. Estrogens play a critical role in reproduction but also have important actions in non-reproductive organs. Estrogen biosynthesis and signaling pathways are locally activated during physiological wound healing, processes that are inhibited in elderly estrogen-deprived skin. Estrogen deprivation has been shown to be a critical mediator of impaired wound healing in both postmenopausal women and aged men, and topical estrogen application reverses age-associated delayed wound healing in both elderly men and women. These data indicate that adequate estrogen biosynthesis and properly regulated estrogen signaling pathways are essential for normal wound healing and can be targeted to optimize tissue repair in the elderly. However, due to fundamental questions regarding how to safely restore estrogen signaling locally in skin wounds, there are currently no therapeutic strategies addressing estrogen deficiency in elderly chronic wounds. This review discusses established and recent literature in this area and proposes the hypothesis that estrogen plays a pleiotropic role in skin aging and that targeting estrogen signaling and biosynthesis could promote skin repair in older adults.
Enhancing physiological metrics, yield, zinc bioavailability, and economic viability of Basmati rice through nano zinc fertilization and summer green manuring in semi–arid South Asian ecosystem
Authors: Baral Kirttiranjan , Shivay Yashbir Singh , Prasanna Radha , Kumar Dinesh , Srinivasarao Cherukumalli , Mandi Sunil , Nayak Somanath , Reddy Kadapa Sreenivasa
Published: October 2023

During the summer and rainy seasons (April-October) of 2020 and 2021, two consecutive field experiments were conducted at the research farm of the ICAR-Indian Agricultural Research Institute, New Delhi, India. In this study, we examined the effects of summer green manuring crops (GM) and a variety of zinc fertilizers (ZnF) onBasmatirice (Oryza sativaL.) growth, physiological development, yield response, zinc nutrition and economic returns. A combination of GM residues and nano zinc fertilization helped significantly enhancingBasmatirice’s growth and its physiological development. Following the incorporation ofSesbania aculeata(Sesbania), successiveBasmatirice physiological parameters were significantly improved, as well as grain, straw, biological yields, harvest index and economic returns. The highest Zn content of 15.1 mg kg-1and the lowest of 11.8 mg kg-1in milled rice grain were recorded inSesbaniagreen manuring (G2) and control i.e., in the fallow (G1), respectively. Coating onto urea with 0.2% nano zinc oxide (NZnCU) was observed to be more effective than other zinc sources in terms of growth parameters, yield attributes, zinc nutrition, grain and straw yields for succeedingBasmatirice crop; however, the effects were comparable to those of bulk zinc oxide-coated urea (BZnCU) of 1%. The highest Zn content of 15.1 mg kg-1was recorded with the application of 1% BZnCU and the lowest of 11.96 mg kg-1with the soil application of 5 kg Zn ha-1through bulk ZnO in the milled rice grain. Application of 1% BZnCU led to a 26.25% increase in Zn content of milled rice grain compared to soil application of 5 kg Zn ha-1through bulk ZnO. As a result, the combination of inclusion ofSesbania aculeata(Sesbania) residue and 0.2% NZnCU was identified as the most effective treatment, for Basmati rice growth and physiological development. A combination of nano Zn fertilization in conjunction with the incorporation of green manure can be advocated for better growth, physiological performance, zinc dense grains, and higher profitability of Basmati rice for farmers and consumers.
Efficacy of the visual cognitive assessment test for mild cognitive impairment/mild dementia diagnosis: a meta-analysis
Authors: Hsu Jui-Hung , Liu Chien-Cheng , Chen I-Wen , Wu Jheng-Yan , Huang Po-Yu , Liu Ting-Hui , Hung Kuo-Chuan
Published: October 2023

Background

Mild cognitive impairment (MCI) is an intermediate stage between normal ageing and dementia. The early identification of MCI is important for timely intervention. The visual cognitive assessment test (VCAT) is a brief language-neutral screening tool for detecting MCI/mild dementia. This meta-analysis evaluated the diagnostic efficacy of the VCAT for MCI/mild dementia.

Methods

Medline, Embase, Google Scholar, and Cochrane Library were searched from their inception until August 2023 to identify studies using VCAT to diagnose MCI/mild dementia. The primary outcome was to assess the diagnostic accuracy of the VCAT for detecting MCI/mild dementia through area under the receiver operating characteristic curve (AU-ROC) analysis. The secondary outcome was to explore the correlation between VCAT scores and MCI/mild dementia presence by comparing scores among patients with and without MCI/mild dementia. Pooled sensitivity, specificity, and area under the curve (AUC) were calculated.

Results

Five studies with 1,446 older adults (mean age 64–68.3 years) were included. The percentage of participants with MCI/mild dementia versus controls ranged from 16.5% to 87% across studies. All studies were conducted in Asian populations, mostly Chinese, in Singapore and Malaysia. The pooled sensitivity was 80% [95% confidence interval (CI) 68%–88%] and the specificity was 75% (95% CI 68%–80%). The AU-ROCC was 0.77 (95% CI 0.73–0.81). Patients with MCI/mild dementia had lower VCAT scores than the controls (mean difference −6.85 points, p < 0.00001).

Conclusion

VCAT demonstrated acceptable diagnostic accuracy in distinguishing MCI/mild dementia in cognitively normal older adults. As a language-neutral and culturally unbiased tool, the VCAT shows promise in detecting MCI/mild dementia. Further studies in non-Asian populations are required.

Systematic review registration

https://www.crd.york.ac.uk/prospero/, identifier: CRD42023453453.
Individuals with developmental disabilities make their own stylistic contributions to text written with physical facilitation
Authors: Nicoli Giovanni , Pavon Giulia , Grayson Andy , Emerson Anne , Cortelazzo Michele , Mitra Suvobrata
Published: October 2023

Introduction

For individuals with developmental disabilities (DD) such as autism, Down syndrome, or cerebral palsy, learning to express with language is a two-fold challenge because atypical cognitive capacity is compounded by sensorimotor coordination deficits. One approach to assisting linguistic expression in these individuals is to physically support them, for example, by touching their torso or arm as they type. The neurophysiological mechanism of such motor assistance for linguistic expression is not known, but recently it has been proposed that light touch may reduce the cognitive load associated with the sensorimotor coordination of typing, thereby releasing shared cognitive resources to the task of generating content. Historically, there has been significant controversy over the extent to which the facilitator and not the user authors texts written with touch assistance. User groups and a few researchers have argued that the user can express their thoughts through such techniques, but the prevailing view among researchers is that these texts are entirely the by-products of the facilitators' ideomotor cueing of users' movements. If the user is not a source of the produced text, the only linguistic style detectable in the text should be the facilitator's.

Methods

Here, we use quantitative linguistic analysis to investigate whether DD users typing text with touch assistance exhibit their own stylistic signatures alongside those of their facilitators. In Study 1, we investigate whether the stylometric fingerprints of a set of users are detectable when they are all assisted by the same facilitator. In Study 2, we examine whether the users' stylometric characteristics are retained even when they are assisted by multiple facilitators.

Results

Across both studies, the results show that the users' stylistic signature is detectable alongside that of facilitators. This suggests that the texts generated by DD users withphysical assistance should be viewed as coauthored rather than wholly authored by facilitators via ideomotor processes.

Discussion

The users' stylometric presence in these texts suggests that touch-assistance may serve as a developmental scaffold and should be re-appraised as a teaching aid even where unassisted linguistic expression is an unlikely end goal.
Comparison of web-based information about cell-free DNA prenatal screening: implications for differences of sex development care
Authors: Kim Soojin , Finney Esther L. , Naha Ushasi , Rosoklija Ilina , Honegger Kyle S. , Goetsch Weisman Allison , Holl Jane L. , Finlayson Courtney , Chen Diane , Johnson Emilie K.
Published: October 2023

Objective

Cell-free DNA (cfDNA) prenatal screening is a commercially available noninvasive test that detects fetal genetic material in maternal blood. While expectant parents often use it for “gender” determination, there is little information about unintended consequences of testing, such as revelation of a difference of sex development (DSD). The study aimed to characterize currently available website information about cfDNA and compare the cfDNA-related content.

Methods

A systematic search for websites with information about cfDNA was conducted using search terms generated by a natural language processing analysis of the results of an Amazon Mechanical Turk (MTurk) survey of 1,000 parents and then performing a “Google” search, using the terms. Commercial cfDNA testing companies (CC) websites were also identified by consulting a genetic counselor (AGW). Data were collected on about each website’s characteristics and information about cfDNA. Information about cfDNA was compared between websites. Data were analyzed using descriptive statistics, Fisher’s exact test or Kruskal-Wallis test were applied, as appropriate.

Results

Sixty websites were identified. After eliminating duplicates, 11 commercial company (CC) websites were identified. Nineteen other websites were reviewed of which six overlapped with five CC websites. Most of the websites had non-professional authors (73.7%), such as laypersons and CC representatives. CC websites were significantly more likely than search term-identified websites to state that cfDNA can screen for trisomy 21 (p=0.002), trisomy 18 (p<0.0001), trisomy 13 (p<0.001), sex chromosome aneuploidies (p<0.001), and microdeletions (p=0.002).

Conclusions

This study shows that most website currently available information for expectant parents about cfDNA prenatal screening is produced by non-professional organizations. There are significant differences between the information provided by CC and Google search websites, specifically about the number of conditions screened for by cfDNA. Improving availability and quality of information about cfDNA could improve counseling future expectant parents. Inclusion of information about the potential for detection of a DSD is needed.
Trends and Opportunities in Social Entrepreneurship Education Research
Authors: Azqueta Arantxa , Sanz-Ponce Roberto , Núñez-Canal Margarita
Published: October 2023

The objective is analyzing the trends in Social Entrepreneurship Education as a multidisciplinary research field. A systematic review of the literature on the intersection: Education and Social Entrepreneurship, with the support of scientific literature and a careful search methodology. It collects articles from the WOS Core collection database published between 2000 and 2022. A total of 367 articles are analyzed to answer the three research questions. The results of the analysis are twelve dimensions identified according to the literature in the field of social entrepreneurship education; after using lexicometric analysis and Iramuteq software, the main trends on the topics are found and discussed and the relationship of terms and concepts in the abstract and title text of the analyzed articles is shown, showing the frequency, importance of linkage, and co-occurrences of lexical units. Six clusters of nodes and related terms are confirmed: entrepreneur, development and innovation, education, entrepreneurial university, context, and types of study. These clusters show the concern for the field of study of social entrepreneurship education and the need to find a consensus on the concept of an entrepreneur and on what is social entrepreneurship in education. The wide range of topics, dispersed and fragmented, continues to offer opportunities for specificity.
Employee Perspectives of Leader Value Orientations, Affect, Trust, and Work Intentions
Authors: Egan Richard , Zigarmi Drea
Published: October 2023

This empirical study on employee perspectives of latent leader value orientations (LVOs), employee psychological job states, and work intentions deployed an online survey to 944 employees within global organizations. Empirical analysis using structural equation modeling confirmed that employee job state positive affect fully mediated relations between LVOs and employee work intentions more so than employee job state negative affect and cognitive-based and affective-based trust in leader. LVO1 (low self-concern and high other-orientation) triggered positive employee psychological job states of greater magnitude than LVO2 (high self-concern and high other-orientation). This finding offers new insight relating to the influence of high leader other-orientation on employee psychological experiences of work considering LVO2 had been reported as ideal. LVO3 (high self-concern and low other-orientation) had the strongest differential associations with employee psychological job states implying that leaders who are perceived by employees to be driven by high self-concern, even in the presence of low other-orientation, evoke strong negative employee psychological responses. Implications for theory and practical strategies to develop leader other-orientation in organizations are presented.
Developing a Regional Network for the Assessment of Evapotranspiration
Authors: Lopez-Guerrero Alicia , Cabello-Leblic Arantxa , Fereres Elias , Vallee Domitille , Steduto Pasquale , Jomaa Ihab , Owaneh Osama , Alaya Itidel , Bsharat Mahmoud , Ibrahim Ayman , Abla Kettani , Mosad Alaa , Omari Abdallah , Zitouna-Chebbi Rim , Jimenez-Berni Jose A.
Published: October 2023

Determining evapotranspiration (ET) is essential for water accounting and for the management of irrigated agriculture from farm to region. We describe here a collaborative initiative aimed at establishing a prototype ET network in six countries of North Africa and the Near East (NENA region). The network utilizes a low-cost and open-source system, termed the CORDOVA-ET, consisting of a base station and sensing nodes to collect the weather data needed to determine the reference and actual ET (ETo and ETa). Here, we describe the network-deployment processes, system architecture, data-collection methodology, quality-control procedures, and some of the ET results obtained so far during a four-year period, starting in 2018. The network has been developed as an iterative and collaborative process, where training and capacity building have been the main drivers. The feedback and experiences gathered from the users have helped improve the different versions of the prototypes and enhance their assembly, deployment, reliability, and ease of operation. At the same time, the involvement in the construction, maintenance, and data analysis has also provided valuable insight into calculating ET from energy-balance methods. The network operated during six cropping seasons and the results were mixed, while data integrity (hourly and daily) varied from 95 to 23% depending on the country and season. Validation of the ET estimates was performed using the ECMWF ERA5 dataset as an independent reference. The energy-balance algorithm implemented in the system to determine the ETa was validated using the OpenCropLib Python library. While the results of the data validation demonstrated the reliability and accuracy of the CORDOVA-ET system, network operations required significant support and special motivation on the part of the users. It is concluded that collaboration among users, together with the support services and participation of different stakeholders interested in agricultural water management, would be essential elements to ensure the sustainability of the ET network.
Expression, Regulation, and Function of β-Defensins in the Bovine Mammary Glands: Current Knowledge and Future Perspectives
Authors: Daneshi Mojtaba , Caton Joel S. , Caixeta Luciano S. , Eftekhari Zohre , Ward Alison K.
Published: October 2023

β-Defensins are cationic antimicrobial peptides (AMPs) that play an important role in the innate immune defense of bovines. They are constitutively expressed in mammary glands and induced differently in response to pathogens. Their expression is influenced by various factors, including hormones, plant-derived compounds, and dietary energy imbalance. The toll-like receptors (TLRs)/nuclear factor-kappa B (NF-κB) pathway plays a crucial role in β-defensin induction, while alternative pathways such as mitogen-activated protein kinase (MAPK) and epigenetic regulation also make substantial contributions. β-Defensins exhibit bactericidal activity against a wide range of pathogens, including two major mastitis pathogens, Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), primarily through membrane disruption. β-Defensins have low cytotoxicity to host cells and demonstrate immunomodulatory properties, and pathogens also display minimal resistance to these AMPs. Given the increasing concern in antimicrobial resistance, the potential of β-defensins as natural antimicrobials has garnered considerable attention. This article provides an overview of the characteristics of bovine β-defensins, their expression pathways, their mode of action, and factors influencing their expression in the mammary glands of cattle. Additionally, it identifies the current gaps in research within this field and suggests areas that require further investigation. Understanding the regulation and function of β-defensins offers valuable insights to develop effective strategies for strengthening the immune system of mammary glands, reducing the reliance on synthetic antimicrobials, and explore novel natural antimicrobial alternatives.
Reaction to Novel Objects and Fecal Glucocorticoid Metabolite Levels in Two Species of Nocturnal Geckos
Authors: Fernández-Lázaro Gloria , Latorre Roberto , Fontanillas Pérez Juan Carlos , Barja Isabel
Published: October 2023

Many reptiles are maintained in captivity and heavily traded, although welfare measures for many species are not well established and are under-researched compared to other animals. In this study, we focused on two of these species: crested geckos (Correlophus ciliatus) and leopard geckos (Eublepharis macularius). To better interpret their behavior in captivity, the individual reaction to novel objects and the fecal glucocorticoid metabolite levels were measured in an attempt to identify the potential correlation between them. Also, we explored if some characteristic of the objects (e.g., color, shape, or smell) resulted in being more attractive to some species and/or individuals. Equivalent responses to different objects were not obtained for all the geckos, the behavioral response being highly individual and context-dependent, although modulated by the species. Individuals which manipulated earlier and interacted longer with novel objects showed lower basal fecal corticosterone metabolite (FCM) levels. Differences according to the species suggested that crested geckos have significantly greater and more variable FCM levels than leopard geckos. Our results can help to understand the reaction of geckos to novelty and have the potential to serve in their welfare assessment, although more studies are needed to proper establish welfare protocols.
Status of Mitochondrial Oxidative Phosphorylation during the Development of Heart Failure
Authors: Bhullar Sukhwinder K. , Dhalla Naranjan S.
Published: October 2023

Mitochondria are specialized organelles, which serve as the “Power House” to generate energy for maintaining heart function. These organelles contain various enzymes for the oxidation of different substrates as well as the electron transport chain in the form of Complexes I to V for producing ATP through the process of oxidative phosphorylation (OXPHOS). Several studies have shown depressed OXPHOS activity due to defects in one or more components of the substrate oxidation and electron transport systems which leads to the depletion of myocardial high-energy phosphates (both creatine phosphate and ATP). Such changes in the mitochondria appear to be due to the development of oxidative stress, inflammation, and Ca2+-handling abnormalities in the failing heart. Although some investigations have failed to detect any changes in the OXPHOS activity in the failing heart, such results appear to be due to a loss of Ca2+ during the mitochondrial isolation procedure. There is ample evidence to suggest that mitochondrial Ca2+-overload occurs, which is associated with impaired mitochondrial OXPHOS activity in the failing heart. The depression in mitochondrial OXPHOS activity may also be due to the increased level of reactive oxygen species, which are formed as a consequence of defects in the electron transport complexes in the failing heart. Various metabolic interventions which promote the generation of ATP have been reported to be beneficial for the therapy of heart failure. Accordingly, it is suggested that depression in mitochondrial OXPHOS activity plays an important role in the development of heart failure.
Feasibility of Shoulder Kinematics Assessment Using Magnetic Inertial Measurement Units in Hemiplegic Patients after Stroke: A Pilot Study
Authors: Longhi Maria , Donati Danilo , Mantovani Monica , Casarotti Silvia , Calbucci Lucia , Puglisi Giulia , Platano Daniela , Benedetti Maria Grazia
Published: October 2023

Scapulothoracic movements are altered after stroke, with resulting shoulder dysfunction. The scapulohumeral rhythm (SHR) is complex and poorly studied. Magnetic inertial measurement units (MIMUs) allow a rapid and accurate analysis of shoulder kinematics. MIMUs were used to assess the SHR during active shoulder flexion and abduction of over 60°. SHR values obtained from the hemiplegic shoulders of stroke patients (n = 7) were compared with those from healthy controls (n = 25) and correlated with clinical–functional measurements. The impairment of paretic arms was assessed using the Fugl-Meyer Assessment (FMA). We found that in paretic shoulders, the scapular tilt was significantly lower at maximal arm flexion and at 60° and 90° of arm abduction. On the paretic side, the SHR was also consistently lower for all measured arm movements. The FMA was correlated with the scapular anterior–posterior tilt at 60° and 90° of shoulder abduction (Rho = 0.847, p = 0.016, and Rho = 0.757, p = 0.049, respectively). This pilot study demonstrates the feasibility of MIMUs in assessing SHR in stroke patients and confirms previous findings on scapular dysfunction in stroke patients.