bluuebunny/crossref_metadata_2025 huggingface dataset:
Uveal Melanoma Patients Have a Distinct Metabolic Phenotype in Peripheral Blood
Authors: de Bruyn Daniël P. , Bongaerts Michiel , Bonte Ramon , Vaarwater Jolanda , Meester-Smoor Magda A. , Verdijk Robert M. , Paridaens Dion , Naus Nicole C. , de Klein Annelies , Ruijter George J. G. , Kiliç Emine , Brosens Erwin
Published: March 2023

Uveal melanomas (UM) are detected earlier. Consequently, tumors are smaller, allowing for novel eye-preserving treatments. This reduces tumor tissue available for genomic profiling. Additionally, these small tumors can be hard to differentiate from nevi, creating the need for minimally invasive detection and prognostication. Metabolites show promise as minimally invasive detection by resembling the biological phenotype. In this pilot study, we determined metabolite patterns in the peripheral blood of UM patients (n = 113) and controls (n = 46) using untargeted metabolomics. Using a random forest classifier (RFC) and leave-one-out cross-validation, we confirmed discriminatory metabolite patterns in UM patients compared to controls with an area under the curve of the receiver operating characteristic of 0.99 in both positive and negative ion modes. The RFC and leave-one-out cross-validation did not reveal discriminatory metabolite patterns in high-risk versus low-risk of metastasizing in UM patients. Ten-time repeated analyses of the RFC and LOOCV using 50% randomly distributed samples showed similar results for UM patients versus controls and prognostic groups. Pathway analysis using annotated metabolites indicated dysregulation of several processes associated with malignancies. Consequently, minimally invasive metabolomics could potentially allow for screening as it distinguishes metabolite patterns that are putatively associated with oncogenic processes in the peripheral blood plasma of UM patients from controls at the time of diagnosis.
Molecular Mechanisms and Pathophysiological Significance of Eryptosis
Authors: Alghareeb Sumiah A. , Alfhili Mohammad A. , Fatima Sabiha
Published: March 2023

Despite lacking the central apoptotic machinery, senescent or damaged RBCs can undergo an unusual apoptosis-like cell death, termed eryptosis. This premature death can be caused by, or a symptom of, a wide range of diseases. However, various adverse conditions, xenobiotics, and endogenous mediators have also been recognized as triggers and inhibitors of eryptosis. Eukaryotic RBCs are unique among their cell membrane distribution of phospholipids. The change in the RBC membrane composition of the outer leaflet occurs in a variety of diseases, including sickle cell disease, renal diseases, leukemia, Parkinson’s disease, and diabetes. Eryptotic erythrocytes exhibit various morphological alterations such as shrinkage, swelling, and increased granulation. Biochemical changes include cytosolic Ca2+ increase, oxidative stress, stimulation of caspases, metabolic exhaustion, and ceramide accumulation. Eryptosis is an effective mechanism for the elimination of dysfunctional erythrocytes due to senescence, infection, or injury to prevent hemolysis. Nevertheless, excessive eryptosis is associated with multiple pathologies, most notably anemia, abnormal microcirculation, and prothrombotic risk; all of which contribute to the pathogenesis of several diseases. In this review, we provide an overview of the molecular mechanisms, physiological and pathophysiological relevance of eryptosis, as well as the potential role of natural and synthetic compounds in modulating RBC survival and death.
LOX-1 Activation by oxLDL Induces AR and AR-V7 Expression via NF-κB and STAT3 Signaling Pathways Reducing Enzalutamide Cytotoxic Effects
Authors: Duprat Felix , Robles Catalina , Castillo María Paz , Rivas Yerko , Mondaca Marcela , Jara Nery , Roa Francisco , Bertinat Romina , Toledo Jorge , Paz Cristian , González-Chavarría Iván
Published: March 2023

The oxidized low-density lipoprotein receptor 1 (LOX-1) is one of the most important receptors for modified LDLs, such as oxidated (oxLDL) and acetylated (acLDL) low-density lipoprotein. LOX-1 and oxLDL are fundamental in atherosclerosis, where oxLDL/LOX1 promotes ROS generation and NF-κB activation inducing the expression of IL-6, a STAT3 activator. Furthermore, LOX-1/oxLDL function has been associated with other diseases, such as obesity, hypertension, and cancer. In prostate cancer (CaP), LOX-1 overexpression is associated with advanced stages, and its activation by oxLDL induces an epithelial-mesenchymal transition, increasing angiogenesis and proliferation. Interestingly, enzalutamide-resistant CaP cells increase the uptake of acLDL. Enzalutamide is an androgen receptor (AR) antagonist for castration-resistant prostate cancer (CRPC) treatment, and a high percentage of patients develop a resistance to this drug. The decreased cytotoxicity is promoted in part by STAT3 and NF-κB activation that induces the secretion of the pro-inflammatory program and the expression of AR and its splicing variant AR-V7. Here, we demonstrate for the first time that oxLDL/LOX-1 increases ROS levels and activates NF-κB, inducing IL-6 secretion and the activation of STAT3 in CRPC cells. Furthermore, oxLDL/LOX1 increases AR and AR-V7 expression and decreases enzalutamide cytotoxicity in CRPC. Thus, our investigation suggests that new factors associated with cardiovascular pathologies, such as LOX-1/oxLDL, may also promote important signaling axes for the progression of CRPC and its resistance to drugs used for its treatment.
Role of Heterogeneous Nuclear Ribonucleoproteins in the Cancer-Immune Landscape
Authors: Sudhakaran Meenakshi , Doseff Andrea I.
Published: March 2023

Cancer remains the second leading cause of death, accounting for approximately 20% of all fatalities. Evolving cancer cells and a dysregulated immune system create complex tumor environments that fuel tumor growth, metastasis, and resistance. Over the past decades, significant progress in deciphering cancer cell behavior and recognizing the immune system as a hallmark of tumorigenesis has been achieved. However, the underlying mechanisms controlling the evolving cancer-immune landscape remain mostly unexplored. Heterogeneous nuclear ribonuclear proteins (hnRNP), a highly conserved family of RNA-binding proteins, have vital roles in critical cellular processes, including transcription, post-transcriptional modifications, and translation. Dysregulation of hnRNP is a critical contributor to cancer development and resistance. HnRNP contribute to the diversity of tumor and immune-associated aberrant proteomes by controlling alternative splicing and translation. They can also promote cancer-associated gene expression by regulating transcription factors, binding to DNA directly, or promoting chromatin remodeling. HnRNP are emerging as newly recognized mRNA readers. Here, we review the roles of hnRNP as regulators of the cancer-immune landscape. Dissecting the molecular functions of hnRNP will provide a better understanding of cancer-immune biology and will impact the development of new approaches to control and treat cancer.
New Insight in Cardiorenal Syndrome: From Biomarkers to Therapy
Authors: Gallo Giovanna , Lanza Oreste , Savoia Carmine
Published: March 2023

Cardiorenal syndrome consists in the coexistence of acute or chronic dysfunction of heart and kidneys resulting in a cascade of feedback mechanisms and causing damage to both organs associated with high morbidity and mortality. In the last few years, different biomarkers have been investigated with the aim to achieve an early and accurate diagnosis of cardiorenal syndrome, to provide a prognostic role and to guide the development of targeted pharmacological and non-pharmacological therapies. In such a context, sodium-glucose cotransporter 2 (SGLT2) inhibitors, recommended as the first-line choice in the management of heart failure, might represent a promising strategy in the management of cardiorenal syndrome due to their efficacy in reducing both cardiac and renal outcomes. In this review, we will discuss the current knowledge on the pathophysiology of cardiorenal syndrome in adults, as well as the utility of biomarkers in cardiac and kidney dysfunction and potential insights into novel therapeutics.
Effect of Hypercholesterolemia, Systemic Arterial Hypertension and Diabetes Mellitus on Peripapillary and Macular Vessel Density on Superficial Vascular Plexus in Glaucoma
Authors: Gomez María Sanz , Zeng Ni , Catagna Catagna Gloria Estefania , Arribas-Pardo Paula , Garcia-Feijoo Julian , Mendez-Hernandez Carmen
Published: March 2023

Background/Aims: Vascular factors are involved in the development of glaucoma, including diseases such as hypercholesterolemia (HC), systemic arterial hypertension (SAH), and diabetes mellitus (DM). The aim of this study was to determine the effect of glaucoma disease on peripapillary vessel density (sPVD) and macular vessel density (sMVD) on the superficial vascular plexus, controlling differences on comorbidities such as SAH, DM and HC between glaucoma patients and normal subjects. Methods: In this prospective, unicenter, observational cross-sectional study, sPVD and sMVD were measured in 155 glaucoma patients and 162 normal subjects. Differences between normal subjects and glaucoma patients’ groups were analyzed. A linear regression model with 95% confidence and 80% statistical power was performed. Results: Parameters with greater effect on sPVD were glaucoma diagnosis, gender, pseudophakia and DM. Glaucoma patients had a sPVD 1.2% lower than healthy subjects (Beta slope 1.228; 95%CI 0.798–1.659, p < 0.0001). Women presented 1.19% more sPVD than men (Beta slope 1.190; 95%CI 0.750–1.631, p < 0.0001), and phakic patients presented 1.7% more sPVD than men (Beta slope 1.795; 95%CI 1.311–2.280, p < 0.0001). Furthermore, DM patients had 0.9% lower sPVD than non-diabetic patients (Beta slope 0.925; 95%CI 0.293–1.558, p = 0.004). SAH and HC did not affect most of the sPVD parameters. Patients with SAH and HC showed 1.5% lower sMVD in the outer circle than subjects without those comorbidities (Beta slope 1.513; 95%CI 0.216–2.858, p = 0.021 and 1.549; 95%CI 0.240–2.858, p = 0.022 respectively. Conclusions: Glaucoma diagnosis, previous cataract surgery, age and gender seem to have greater influence than the presence of SAH, DM and HC on sPVD and sMVD, particularly sPVD.
A Hybrid–Source Ranging Method in Shallow Water Using Modal Dispersion Based on Deep Learning
Authors: Wang Tong , Su Lin , Ren Qunyan , Li He , Jia Yuqing , Ma Li
Published: March 2023

The relationship between modal elevation angle and the relative arrival time between modes, derived from exploiting modal dispersion, provides source information that is less susceptible to environmental influences. However, the standard method based on modal dispersion has limitations for application. To overcome this, we propose a hybrid method for passive source ranging of low-frequency underwater acoustic-pulse signals in a range-independent shallow-water waveguide. Our method leverages deep learning, utilizing the intermediate results from the standard method as inputs, and short-time conventional beamforming to transform signals received by a vertical line array into a beam-time-domain sound-intensity map. The source range is estimated using an attention-based regression model with a ResNet backbone that has been trained on the beam-time-domain sound-intensity map. Our experimental results demonstrate the superiority of the proposed method, with a mean relative-error reduction of 71%, mean root-squared error reduction of 2.25 km, and an accuracy of 85%, compared to matched-field processing.
Enhancing Power Transmission Stability of AUV’s Wireless Power Transfer System with Compact Planar Magnetic Coupler
Authors: Wen Haibing , Li Jiayuan , Zhang Kehan , Ye Jinying , Yan Zhengchao , Song Baowei , Tong Xiangqian
Published: March 2023

In this paper, a wireless power transfer (WPT) system with a compact planar magnetic coupler for an autonomous underwater vehicle (AUV) is proposed. A passive induction (PI) coil is integrated into the circular transmitter (Tx) coil to build a uniform magnetic field (UMF), which can guarantee the stable output of the WPT system under uncertain radial and axial misalignments for AUV. Based on normalized magnetic induction intensity distribution analysis, a UMF constructing method with a PI coil is given, aiming to eliminate the fluctuation of magnetic field intensity, and the PI coil design principles and flow chart are obtained. The theoretical analysis shows the proposed integrated coil can effectively enhance the radial misalignment tolerance compared with a conventional circular spiral coil. The zero-phase angle (ZPA) input condition can be achieved by adjusting the series capacitor connected with the Tx coil in S-S compensation topology. Experimental results show that the proposed magnetic coupler containing an integrated coil significantly improves the stability of output power and power transfer efficiency within the possible radial and axial misalignments compared with a conventional coupler. It was demonstrated that the output power changes less than 5.5% and the power transfer efficiency maintains at approximately 84.5% in arbitrary radial positions within the possible working region with an axial transfer distance of 50 mm in saltwater.
Different Species of Epigeic and Anecic Earthworms Cause Similarly Effective and Beneficial Biocomposting—A Case Study Involving the Pernicious Aquatic Weed Salvinia (Salvinia molesta, Mitchell)
Authors: Tabassum-Abbasi  , Hussain Naseer , Khamrang Channgam , Patnaik Pratiksha , Abbasi Tasneem , Abbasi Shahid Abbas
Published: March 2023

As reported recently by the present authors, vermicomposting by the epigeic earthworm Eisenia fetida transforms the highly ligninous and allelopathic aquatic weed salvinia (Salvinia molesta) into a benign organic fertilizer. The present study was carried out with four other earthworm species, including three epigeic species of different sizes and phytophagic habits: Eisenia andrei, Lumbricus rubillus, and Perionyx sansibaricus. One anecic species, with geophytophagous habits, was also explored for comparison: Drawida willsi. The objective was to see whether the type of salvinia transformation caused by E. fetida is a general phenomenon or whether there are significant differences in the nature of biocomposts generated by different earthworm species. Accordingly, the characteristics of the biocomposts separately generated by each of the six species mentioned above were assessed with UV-visible spectrophotometry, Fourier-transform infrared spectrometry, differential scanning calorimetry, thermogravimetry, and scanning electron microscopy. The studies reveal that, with minor variations, the biocomposting by all four species was able to remove the intransigence of salvinia and impart plant/soil-friendly attributes to it in substantial measures. All the findings obtained with different techniques corroborated each other in arriving at this conclusion. Hence, it can be said that, in general, biocomposting by earthworms takes away the toxicity of pernicious weeds such as salvinia, converting them into plant-friendly and soil-friendly biofertilizers.
Influence of Polycaprolactone Concentration and Solvent Type on the Dimensions and Morphology of Electrosprayed Particles
Authors: Alberto Laura , Kalluri Lohitha , Qu Jing , Zhao Yongfeng , Duan Yuanyuan
Published: March 2023

Polycaprolactone (PCL) micro- and nanoparticles produced using the electrospraying technique present high drug encapsulation capacity, a controllable surface area, and a good cost–benefit ratio. PCL is also considered a non-toxic polymeric material with excellent biocompatibility and biodegradability. All these characteristics make PCL micro- and nanoparticles a promising material for tissue engineering regeneration, drug delivery, and surface modification in dentistry. In this study, PCL electrosprayed specimens were produced and analyzed to determine their morphology and size. Three PCL concentrations (2, 4, and 6 wt%) and three solvent types (chloroform (CF), dimethylformamide (DMF), and acetic acid (AA)) with various solvent mixtures ratios (1:1 CF/DMF, 3:1 CF/DMF, 100% CF, 1:1 AA/CF, 3:1 AA/CF, and 100% AA) were used while keeping the remaining electrospray parameters constant. SEM images followed by ImageJ analysis showed a change in the morphology and size of the particles among various tested groups. A two-way ANOVA demonstrated a statistically significant interaction (p < 0.001) between PCL concentration and solvents on the size of the particles. With the increase in the PCL concentration, an increase in the number of fibers was observed among all the groups. The morphology and dimensions of the electrosprayed particles, as well as the presence of fibers, were significantly dependent on the PCL concentration, choice of solvent, and solvent ratio.
Recycling of Tire-Derived Fiber: The Contribution of Steel Cord on the Properties of Lightweight Concrete Based on Perlite Aggregate
Authors: Kadela Marta , Małek Marcin , Jackowski Mateusz , Kunikowski Mateusz , Klimek Agnieszka , Dudek Daniel , Rośkowicz Marek
Published: March 2023

The increasing amount of waste from the vulcanization industry has become a serious environmental challenge. Even the partial reuse of the steel contained in tires as dispersed reinforcement in the production of new building materials may contribute to reducing the environmental impact of this industry while supporting the principle of sustainable development. In this study, the concrete samples were made of Portland cement, tap water, lightweight perlite aggregates, and steel cord fibers. Two different addition of steel cord fibers (1.3% and 2.6% wt. of concrete, respectively) were used. The samples of lightweight concrete based on perlite aggregate with steel cord fiber addition showed a significant increase in compressive (18–48%), tensile (25–52%), and flexural strength (26–41%). Moreover, higher thermal conductivity and thermal diffusivity were reported after incorporating steel cord fibers into the concrete matrix; however, the specific heat values decreased after these modifications. The highest values of thermal conductivity and thermal diffusivity were obtained for samples modified with a 2.6% addition of steel cord fibers and were equal to 0.912 ± 0.002 W/mK and 0.562 ± 0.002 µm2/s, respectively. Maximum specific heat, on the other hand, was reported for plain concrete (R)—1.678 ± 0.001 MJ/m3 K.
A Novel POP-Ni Catalyst Derived from PBTP for Ambient Fixation of CO2 into Cyclic Carbonates
Authors: Wei Fen , Qiu Jiaxiang , Zeng Yanbin , Liu Zhimeng , Wang Xiaoxia , Xie Guanqun
Published: March 2023

The immobilization of homogeneous catalysts has always been a hot issue in the field of catalysis. In this paper, in an attempt to immobilize the homogeneous [Ni(Me6Tren)X]X (X = I, Br, Cl)-type catalyst with porous organic polymer (POP), the heterogeneous catalyst PBTP-Me6Tren(Ni) (POP-Ni) was designed and constructed by quaternization of the porous bromomethyl benzene polymer (PBTP) with tri[2-(dimethylamino)ethyl]amine (Me6Tren) followed by coordination of the Ni(II) Lewis acidic center. Evaluation of the performance of the POP-Ni catalyst found it was able to catalyze the CO2 cycloaddition with epichlorohydrin in N,N-dimethylformamide (DMF), affording 97.5% yield with 99% selectivity of chloropropylene carbonate under ambient conditions (80 °C, CO2 balloon). The excellent catalytic performance of POP-Ni could be attributed to its porous properties, the intramolecular synergy between Lewis acid Ni(II) and nucleophilic Br anion, and the efficient adsorption of CO2 by the multiamines Me6Tren. In addition, POP-Ni can be conveniently recovered through simple centrifugation, and up to 91.8% yield can be obtained on the sixth run. This research provided a facile approach to multifunctional POP-supported Ni(II) catalysts and may find promising application for sustainable and green synthesis of cyclic carbonates.
Wearable Continuous Blood Pressure Monitoring Devices Based on Pulse Wave Transit Time and Pulse Arrival Time: A Review
Authors: Zhou Zi-Bo , Cui Tian-Rui , Li Ding , Jian Jin-Ming , Li Zhen , Ji Shou-Rui , Li Xin , Xu Jian-Dong , Liu Hou-Fang , Yang Yi , Ren Tian-Ling
Published: March 2023

Continuous blood pressure (BP) monitoring is of great significance for the real-time monitoring and early prevention of cardiovascular diseases. Recently, wearable BP monitoring devices have made great progress in the development of daily BP monitoring because they adapt to long-term and high-comfort wear requirements. However, the research and development of wearable continuous BP monitoring devices still face great challenges such as obvious motion noise and slow dynamic response speeds. The pulse wave transit time method which is combined with photoplethysmography (PPG) waves and electrocardiogram (ECG) waves for continuous BP monitoring has received wide attention due to its advantages in terms of excellent dynamic response characteristics and high accuracy. Here, we review the recent state-of-art wearable continuous BP monitoring devices and related technology based on the pulse wave transit time; their measuring principles, design methods, preparation processes, and properties are analyzed in detail. In addition, the potential development directions and challenges of wearable continuous BP monitoring devices based on the pulse wave transit time method are discussed.
Preparation of Hydrogel Composites Using a Sustainable Approach for In Situ Silver Nanoparticles Formation
Authors: Chronopoulou Laura , Binaymotlagh Roya , Cerra Sara , Haghighi Farid Hajareh , Di Domenico Enea Gino , Sivori Francesca , Fratoddi Ilaria , Mignardi Silvano , Palocci Cleofe
Published: March 2023

The recognized antibacterial properties of silver nanoparticles (AgNPs) characterize them as attractive nanomaterials for developing new bioactive materials less prone to the development of antibiotic resistance. In this work, we developed new composites based on self-assembling Fmoc-Phe3 peptide hydrogels impregnated with in situ prepared AgNPs. Different methodologies, from traditional to innovative and eco-sustainable, were compared. The obtained composites were characterized from a hydrodynamic, structural, and morphological point of view, using different techniques such as DLS, SEM, and rheological measurements to evaluate how the choice of the reducing agent determines the characteristics of AgNPs and how their presence within the hydrogel affects their structure and properties. Moreover, the antibacterial properties of these composites were tested against S. aureus, a major human pathogen responsible for a wide range of clinical infections. Results demonstrated that the hydrogel composites containing AgNPs (hgel@AgNPs) could represent promising biomaterials for treating S. aureus-related infections.
Effect of Sintering Temperature and Solution Treatment on Phase Changes and Mechanical Properties of High-Nitrogen Stainless Steel Prepared by MIM
Authors: Zhang Weipeng , Li Liejun , Huang Chengcheng , Ngai Tungwai , Hu Ling
Published: March 2023

High-nitrogen stainless steel (HNSS) has been widely concerned and studied owing to its excellent mechanical, corrosion resistance, and biocompatibility properties. A series of HNSS was prepared by metal injection molding (MIM) using gas atomized Fe–Cr–Mn–Mo–0.3 N duplex stainless steel powders. Both sintering and solution treatments were carried out in an N2 atmosphere. The effects of nitrogen distribution and phase transformation on the mechanical properties of MIM HNSS during sintering and solution were studied. The results show that as the sintering temperature increased, the sample density increased, but the total nitrogen content decreased. Nitrogen and Cr2N concentration gradients along the cross-section of as-sintered samples were formed after cooling. The high nitrogen content promotes the decomposition of γ: γsaturated translated to γ and Cr2N. Meanwhile, the low Mn content in austenite also decomposes γ: γ translated to α and Cr2N. After solution treatment, a single γ phase was obtained for samples sintered at 1200 to 1320 °C. For solution treatment samples sintered at 1320 and 1350 °C, their tensile strength was 988.76 and 1036.12 MPa; yield strength was 615.61 and 636.14 MPa, and elongation was 42.58 and 40.08%, respectively. These values vastly exceeded the published MIM HNSS values.
Study of the Impact of Coals and Claystones on Wear-Resistant Steels
Authors: Wieczorek Andrzej N. , Jonczy Iwona , Filipowicz Krzysztof , Kuczaj Mariusz , Pawlikowski Arkadiusz , Łukowiec Dariusz , Staszuk Marcin , Gerle Anna
Published: March 2023

This paper discusses the impact of coal abrasive materials of varied petrographic composition and claystones containing admixtures of coal matter on the surface wear of wear-resistant martensitic steels. Wear tests were conducted at a test stand for three petrographic varieties of hard coal: vitrinite, clarinite, and durinite, and five samples of claystone. These tests revealed no significant effect of the type of coal abrasive used on the value of mass loss from the surface of the wear-resistant steel samples. The reason behind the foregoing is the observed tendency of coal abrasives, irrespective of their petrographic variety, to penetrate surface irregularities, especially those attributable to previous surface treatment of the samples and the impact of wear products. The dominant forms of surface damage were surface fatigue chipping and scratches caused by the particles which detached themselves from the surface of the steel samples, as observed for all the analysed coal variants. On the surfaces of the samples seasoned in the presence of claystones, highly varied forms of damage were observed: microcutting, scaly surface cracks, delamination, and deep cracks. In these cases, it was possible that the abrasive grains had been pressed into the steel surface irregularities, but no layered forms of the pressed-in abrasive material were observed to have developed. The paper also presents a model for the formation of coal films and discusses their possible effect on wear minimisation.
Investigation of Thermal Sensing in Fluoroindate Yb3+/Er3+ Co-Doped Optical Fiber
Authors: Starzyk Bartłomiej , Jimenez Gloria Lesly , Kochanowicz Marcin , Kuwik Marta , Żmojda Jacek , Miluski Piotr , Baranowska Agata , Dorosz Jan , Pisarski Wojciech , Pisarska Joanna , Dorosz Dominik
Published: March 2023

An investigation of fluoroindate glass and fiber co-doped with Yb3+/Er3+ ions as a potential temperature sensor was assessed using the fluorescence intensity ratio (FIR) technique. Analysis of thermally coupled levels (TCLs—2H11/2 and 4S3/2), non-thermally coupled levels (non-TCLs—4F7/2 and 4F9/2), and their combination were examined. Additionally, the luminescent stability of the samples under constant NIR excitation using different density power at three different temperatures was carried out. The obtained values of absolute sensitivity (0.003 K−1—glass, 0.0019 K−1—glass fiber 2H11/2 → 4S3/2 transition) and relative sensitivity (2.05% K−1—glass, 1.64% K−1—glass fiber 4F7/2 → 4F9/2 transition), as well as high repeatability of the signal, indicate that this material could be used in temperature sensing applications.
Ultra-High-Molecular-Weight Polyethylene in Hip and Knee Arthroplasties
Authors: Hasegawa Masahiro , Tone Shine , Naito Yohei , Sudo Akihiro
Published: March 2023

Ultra-high-molecular-weight polyethylene (UHMWPE) wear and particle-induced osteolysis contribute to the failure of total hip arthroplasty (THA) and total knee arthroplasty (TKA). Highly crosslinked polyethylene (HXLPE) was developed in the late 1990s to reduce wear and has shown lower wear rates and loosening than conventional UHMWPE in THA. The irradiation dose for crosslinking is up to 100 kGy. However, during crosslinking, free radical formation induces oxidation. Using HXLPE in THA, the cumulative revision rate was determined to be significantly lower (6.2%) than that with conventional UHMWPE (11.7%) at a mean follow-up of 16 years, according to the Australian Orthopaedic Association National Joint Replacement Registry. However, HXLPE does not confer to TKA the same advantages it confers to THA. Several alternatives have been developed to prevent the release of free radicals and improve polymer mechanical properties, such as thermal treatment, phospholipid polymer 2-methacryloyloxyethyl phosphorylcholine grafting, remelting, and vitamin E addition. Among these options, vitamin E addition has reported good clinical results and wear resistance similar to that of HXLPE without vitamin E, as shown by short-term clinical studies of THA and TKA. This review aims to provide a comprehensive overview of the development and performance of UHMWPE in THA and TKA.
A New Approach for Classifying the Permanent Deformation Properties of Granular Materials under Cyclic Loading
Authors: Liu Gang , Zhao Mingzhi , Luo Qiang , Zhou Jianchuan
Published: March 2023

A series of medium-sized cyclic triaxial tests were performed to investigate the permanent deformation properties of granular materials. The strain rate was then plotted against loading cycles to classify the permanent deformation properties of granular materials under different cyclic stress ratios (CSRs). It was found that (1) the permanent strain rate dεp/dN was linearly correlated with loading cycles N using a double-log coordinate on the condition of CSR < 60%; (2) the deformation tendency factor β, which was extracted from the linear relationship between dεp/dN and N, significantly varied with CSR and, thus, can be adopted to identify the deformation states; (3) β > 1 implying that permanent strain accumulation ceases in limited cycles and corresponds to the plastic shakedown range, while 0 < β ≤ 1 indicates the temporary steady state, corresponding to the plastic creep range; (4) sluggish decrease or remarkable increase in dεp/dN appeared as CSR ≥ 60%, leading to soil collapsed in limited loading cycles and resulting in an incremental collapse range. The new approach was validated by the crushed tuff aggregates and subgrade materials reported previously. It is expected that the new approach will have wider applicability than the traditional one and can provide technical guidance for the design and construction of substructures in roadway and railway engineering.
Antiferromagnetism and Structure of Sr1−xBaxFeO2F Oxyfluoride Perovskites
Authors: Garcia-Ramos Crisanto A. , Krezhov Kiril , Fernández-Díaz María T. , Alonso José A.
Published: March 2023

Recently, a series of oxyfluorides, Sr1−xBaxFeO2F with x = 0, 0.25, 0.50, and 0.75 obtained through a novel synthesis route, were characterized by X-ray and neutron powder diffraction (NPD), magnetization measurements, and 57Fe Mössbauer spectroscopy (MS). The diffraction data revealed random occupancy of Sr and Ba atoms at the A-cation site, and a statistical distribution of O and F at the anionic sublattice of the perovskite-like structure specified in space group Pm-3m. MS spectra analysis consistently indicated the presence of Fe3+ ions at B-site, confirming the Sr1−xBaxFeO2F stoichiometry. Magnetic structure determination from the NPD data at room temperature established G-type antiferromagnetic arrangement in all compositions with Fe3+ moments of about 3.5 μB oriented along the c axis. In this study, we present and analyze additional NPD data concerning the low-temperature chemical and magnetic structure of Sr0.5Ba0.5FeO2F (x = 0.5) and SrFeO2F (x = 0). Basically, the three-dimensional G-type magnetic structure is maintained down to 2 K, where it is fully developed with an ordered magnetic moment of 4.25(5) μB/Fe at this temperature for x = 0.5 and 4.14(3) μB/Fe for x = 0. The data processing is complemented with a new approach to analyze the temperature dependence of the magnetic order TN on the lattice parameters, based on the magnetic hyperfine fields extracted from the temperature-dependent MS data.
High-Dimensional Distributionally Robust Mean-Variance Efficient Portfolio Selection
Authors: Zhang Zhonghui , Jing Huarui , Kao Chihwa
Published: March 2023

This paper introduces a novel distributionally robust mean-variance portfolio estimator based on the projection robust Wasserstein (PRW) distance. This approach addresses the issue of increasing conservatism of portfolio allocation strategies due to high-dimensional data. Our simulation results show the robustness of the PRW-based estimator in the presence of noisy data and its ability to achieve a higher Sharpe ratio than regular Wasserstein distances when dealing with a large number of assets. Our empirical study also demonstrates that the proposed portfolio estimator outperforms classic “plug-in” methods using various covariance estimators in terms of risk when evaluated out of sample.
Chemical Compositions and Experimental and Computational Modeling of the Anticancer Effects of Cnidocyte Venoms of Jellyfish Cassiopea andromeda and Catostylus mosaicus on Human Adenocarcinoma A549 Cells
Authors: Zare Afshin , Afshar Alireza , Khoradmehr Arezoo , Baghban Neda , Mohebbi Gholamhossein , Barmak Alireza , Daneshi Adel , Bargahi Afshar , Nabipour Iraj , Almasi-Turk Sahar , Arandian Alireza , Zibaii Mohammad Ismail , Latifi Hamid , Tamadon Amin
Published: March 2023

Nowadays, major attention is being paid to curing different types of cancers and is focused on natural resources, including oceans and marine environments. Jellyfish are marine animals with the ability to utilize their venom in order to both feed and defend. Prior studies have displayed the anticancer capabilities of various jellyfish. Hence, we examined the anticancer features of the venom of Cassiopea andromeda and Catostylus mosaicus in an in vitro situation against the human pulmonary adenocarcinoma (A549) cancer cell line. The MTT assay demonstrated that both mentioned venoms have anti-tumoral ability in a dose-dependent manner. Western blot analysis proved that both venoms can increase some pro-apoptotic factors and reduce some anti-apoptotic molecules that lead to the inducing of apoptosis in A549 cells. GC/MS analysis demonstrated some compounds with biological effects, including anti-inflammatory, antioxidant and anti-cancer activities. Molecular docking and molecular dynamic showed the best position of each biologically active component on the different death receptors, which are involved in the process of apoptosis in A549 cells. Ultimately, this study has proven that both venoms of C. andromeda and C. mosaicus have the capability to suppress A549 cells in an in vitro condition and they might be utilized in order to design and develop brand new anticancer agents in the near future.
Metabolomics-Based Analysis of the Effects of Different Cultivation Strategies on Metabolites of Dendrobium officinale Kimura et Migo
Authors: Yang Da , Song Yeyang , Lu Anjin , Qin Lin , Tan Daopeng , Zhang Qianru , He Yuqi , Lu Yanliu
Published: March 2023

Dendrobium officinale Kimura et Migo is a famous plant with a high medicinal value which has been recorded in the Chinese Pharmacopoeia (2020 Edition). The medicinal properties of D. officinale are based on its chemical composition. However, there are no reports on how different cultivation methods affect its chemical composition. In order to reveal this issue, samples of the D. officinale were collected in this study through tree epiphytic cultivation, stone epiphytic cultivation, and greenhouse cultivation. Polysaccharides were determined by phenol sulfuric acid method and secondary metabolites were detected by the UPLC-MS technique. In addition, with regards to metabolomics, we used multivariate analyses including principal component analysis (PCA) and orthogonal partial least squares analysis (OPLS-DA) to screen for differential metabolites which met the conditions of variable importance projection values >1, fold change >4, and p < 0.05. The differential metabolites were taken further for metabolic pathway enrichment analysis, which was based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, and validated by antioxidant activity. Comparing the three groups of samples according to the standards of the ChP (2020 edition), the results showed that the polysaccharide content of the samples from stony epiphytic cultivation and greenhouse cultivation was significantly higher than that of the samples from live tree epiphytic cultivation. Metabolomic analysis revealed that there were 185 differential metabolites among the 3 cultivation methods, with 99 of the differential metabolites being highest in the stone epiphytic cultivation. The results of the metabolic pathway enrichment analysis showed that the different cultivation strategies mainly effected four carbohydrate metabolic pathways, five secondary metabolite synthesis pathways, six amino acid metabolic pathways, one nucleotide metabolism pathway, three cofactor and vitamin metabolism pathways, and one translation pathway in genetic information processing. Furthermore, D. officinale from stone epiphytic cultivation which had the best antioxidant activity was implicated in differential metabolite production. This study revealed the effects of different cultivation methods on the chemical composition of D. officinale and also provided a reference for establishing the quality control standards to aid its development and utilization.
Impact of Stress and Dimension on Nanosheet Deformation during Channel Release of Gate-All-Around Device
Authors: Yang Jingwen , Chen Kun , Wang Dawei , Liu Tao , Sun Xin , Wang Qiang , Huang Ziqiang , Pan Zhecheng , Xu Saisheng , Wang Chen , Wu Chunlei , Xu Min , Zhang David Wei
Published: March 2023

In this paper, nanosheet deformation during channel release has been investigated and discussed in Gate-All-Around (GAA) transistors. Structures with different source/drain size and stacked Si nanosheet lengths were designed and fabricated. The experiment of channel release showed that the stress caused serious deformation to suspended nanosheets. With the guidance of the experiment result, based on simulation studies using the COMSOL Multiphysics and Sentaurus tools, it is confirmed that the stress applied on the channel from source/drain plays an important role in nanosheet deformation during the fabrication process. The deformation of Si nanosheets would cause a serious degradation of the device performance due to an inability to control the work function of the metal gate. This study proposed that the uniformly stacked GAA nanosheets structure could be successfully demonstrated with suitable channel stress engineering provided by fitting S/D size and an appropriate channel length. The conclusions provide useful guidelines for future stacked GAA transistors’ design and fabrication.
Innovative Fabrication of Hollow Microneedle Arrays Enabling Blood Sampling with a Self-Powered Microfluidic Patch
Authors: Van Hileghem Lorenz , Kushwaha Shashwat , Piovesan Agnese , Verboven Pieter , Nicolaï Bart , Reynaerts Dominiek , Dal Dosso Francesco , Lammertyn Jeroen
Published: March 2023

Microneedles are gaining a lot of attention in the context of sampling cutaneous biofluids such as capillary blood. Their minimal invasiveness and user-friendliness make them a prominent substitute for venous puncture or finger-pricking. Although the latter is suitable for self-sampling, the impracticality of manual handling and the difficulty of obtaining enough qualitative sample is driving the search for better solutions. In this context, hollow microneedle arrays (HMNAs) are particularly interesting for completely integrating sample-to-answer solutions as they create a duct between the skin and the sampling device. However, the fabrication of sharp-tipped HMNAs with a high aspect ratio (AR) is challenging, especially since a length of ≥1500 μm is desired to reach the blood capillaries. In this paper, we first described a novel two-step fabrication protocol for HMNAs in stainless steel by percussion laser drilling and subsequent micro-milling. The HMNAs were then integrated into a self-powered microfluidic sampling patch, containing a capillary pump which was optimized to generate negative pressure differences up to 40.9 ± 1.8 kPa. The sampling patch was validated in vitro, showing the feasibility of sampling 40 μL of liquid. It is anticipated that our proof-of-concept is a starting point for more sophisticated all-in-one biofluid sampling and point-of-care testing systems.
High Variation in Protist Diversity and Community Composition in Surface Sediment of Hot Springs in Himalayan Geothermal Belt, China
Authors: Zhang Peng , Xiong Jie , Qiao Nanqian , Luo Shuai , Yang Qing , Li Xiaodong , An Ruizhi , Jiang Chuanqi , Miao Wei , Ba Sang
Published: March 2023

Hot springs are some of the most special environments on Earth. Many prokaryotic and eukaryotic microbes have been found to live in this environment. The Himalayan geothermal belt (HGB) has numerous hot springs spread across the area. Comprehensive research using molecular techniques to investigate eukaryotic microorganisms is still lacking; investigating the composition and diversity of eukaryotic microorganisms such as protists in the hot spring ecosystems will not only provide critical information on the adaptations of protists to extreme conditions, but could also give valuable contributions to the global knowledge of biogeographic diversity. In this study, we used high-throughput sequencing to illuminate the diversity and composition pattern of protist communities in 41 geothermal springs across the HGB on the Tibetan Plateau. A total of 1238 amplicon sequence variants (ASVs) of protists were identified in the hot springs of the HGB. In general, Cercozoa was the phylum with the highest richness, and Bacillariophyta was the phylum with the highest relative abundance in protists. Based on the occurrence of protist ASVs, most of them are rare. A high variation in protist diversity was found in the hot springs of the HGB. The high variation in protist diversity may be due to the different in environmental conditions of these hot springs. Temperature, salinity, and pH are the most important environmental factors that affect the protist communities in the surface sediments of the hot springs in the HGB. In summary, this study provides the first comprehensive study of the composition and diversity of protists in the hot springs of the HGB and facilitates our understanding of the adaptation of protists in these extreme habitats.
Nematode Pheromones: Structures and Functions
Authors: Yang Biyuan , Wang Jie , Zheng Xi , Wang Xin
Published: March 2023

Pheromones are chemical signals secreted by one individual that can affect the behaviors of other individuals within the same species. Ascaroside is an evolutionarily conserved family of nematode pheromones that play an integral role in the development, lifespan, propagation, and stress response of nematodes. Their general structure comprises the dideoxysugar ascarylose and fatty-acid-like side chains. Ascarosides can vary structurally and functionally according to the lengths of their side chains and how they are derivatized with different moieties. In this review, we mainly describe the chemical structures of ascarosides and their different effects on the development, mating, and aggregation of nematodes, as well as how they are synthesized and regulated. In addition, we discuss their influences on other species in various aspects. This review provides a reference for the functions and structures of ascarosides and enables their better application.
Stereoselective Synthesis of 1-Substituted Homotropanones, including Natural Alkaloid (−)-Adaline
Authors: Hernández-Ibáñez Sandra , Sirvent Ana , Yus Miguel , Foubelo Francisco
Published: March 2023

The stereocontrolled synthesis of 1-substituted homotropanones, using chiral N-tert-butanesulfinyl imines as reaction intermediates, is described. The reaction of organolithium and Grignard reagents with hydroxy Weinreb amides, chemoselective N-tert-butanesulfinyl aldimine formation from keto aldehydes, decarboxylative Mannich reaction with β-keto acids of these aldimines, and organocatalyzed L-proline intramolecular Mannich cyclization are key steps of this methodology. The utility of the method was demonstrated with a synthesis of the natural product (−)-adaline, and its enantiomer, (+)-adaline.
Aspirin-Triggered Resolvin D1 (AT-RvD1) Protects Mouse Skin against UVB-Induced Inflammation and Oxidative Stress
Authors: Melo Cristina P. B. , Saito Priscila , Martinez Renata M. , Staurengo-Ferrari Larissa , Pinto Ingrid C. , Rodrigues Camilla C. A. , Badaro-Garcia Stephanie , Vignoli Josiane A. , Baracat Marcela M. , Bussmann Allan J. C. , Georgetti Sandra R. , Verri Waldiceu A. , Casagrande Rubia
Published: March 2023

Intense exposure to UVB radiation incites excessive production of reactive oxygen species (ROS) and inflammation. The resolution of inflammation is an active process orchestrated by a family of lipid molecules that includes AT-RvD1, a specialized proresolving lipid mediator (SPM). AT-RvD1 is derived from omega-3, which presents anti-inflammatory activity and reduces oxidative stress markers. The present work aims to investigate the protective effect of AT-RvD1 on UVB-induced inflammation and oxidative stress in hairless mice. Animals were first treated with 30, 100, and 300 pg/animal AT-RvD1 (i.v.) and then exposed to UVB (4.14 J/cm2). The results showed that 300 pg/animal of AT-RvD1 could restrict skin edema, neutrophil and mast cell infiltration, COX-2 mRNA expression, cytokine release, and MMP-9 activity and restore skin antioxidant capacity as per FRAP and ABTS assays and control O2•− production, lipoperoxidation, epidermal thickening, and sunburn cells development. AT-RvD1 could reverse the UVB-induced downregulation of Nrf2 and its downstream targets GSH, catalase, and NOQ-1. Our results suggest that by upregulating the Nrf2 pathway, AT-RvD1 promotes the expression of ARE genes, restoring the skin’s natural antioxidant defense against UVB exposition to avoid oxidative stress, inflammation, and tissue damage.
Recent Progress in Understanding the Health Benefits of Curcumin
Authors: Porro Chiara , Panaro Maria Antonietta
Published: March 2023

Nutrients and their potential benefits are a new field of study in modern medicine due to their positive impact on health [...]
Recent Advances in In Vitro and In Vivo Studies of Antioxidant, ACE-Inhibitory and Anti-Inflammatory Peptides from Legume Protein Hydrolysates
Authors: Tawalbeh Deia , Al-U’datt Muhammad H. , Wan Ahmad Wan Amir Nizam , Ahmad Fisal , Sarbon Norizah Mhd
Published: March 2023

Consumption of legumes has been shown to enhance health and lower the risk of cardiovascular disease and specific types of cancer. ACE inhibitors, antioxidants, and synthetic anti-inflammatories are widely used today; however, they have several undesirable side effects. Thus, researchers have focused on finding ACE inhibitors, antioxidant, and anti-inflammatory peptides from natural sources, such as legumes. Recently, in vitro and in vivo research has shown the bioactive peptides generated from legume protein hydrolysates, such as antioxidant, anti-hypertensive, anticancer, anti-proliferative, anti-inflammatory, etc., in the context of different disease mitigation. Therefore, this review aims to describe the recent advances in in vitro and in vivo studies of antioxidant, anti-hypertensive and anti-inflammatory peptides isolated from legume-derived protein hydrolysates. The results indicated that antioxidant legumes peptides are characterized by short-chain sequence amino acids and possess anti-hypertensive properties by reducing systolic blood pressure (SBP) in spontaneously hypertensive rats (SHR).