bluuebunny/crossref_metadata_2025 huggingface dataset:
Failure mechanism and control of coal bursts triggered by mining induced seismicity in steeply inclined and extra thick coal seam
Authors: Cao Jinrong , Dou Linming , He Jiang , Zhu Guangan , Wang Zhengyi , Bai Jinzheng , Han Zepeng
Published: January 2023

With the increase in mining depth, coal bursts have become a major challenge in the safe mining of steeply inclined and extra thick coal seams (SIETCSs). Based on a typical mining induced seismicity triggered coal burst (MSTCB) in SIETCS, a large-scale numerical model was developed using the Universal Distinct Element Code. The numerical model was calibrated and validated by laboratory results and field observations. The stress evolution, crack development and ejection velocity patterns in the MSTCB were analysed, and the effect of mining induced seismicity vibration velocity on the MSTCB was discussed. The results show that a triangular static stress concentration zone is formed in the coal on the roof side. And the high-energy mining induced seismicity leads to high dynamic stresses in the coal at the roof side rib and top of the headentry. Coal bursts occur under the superposition of static and dynamic stresses. The MSTCB results in tensile failure near the headentry surface and shear failure in the depth. The vibration velocity has a significant effect on the roof side rib and top of the headentry, while it has only a slight effect on the working face rib and bottom of the headentry. The dynamic stress and ejection velocity in the roof side rib and top of the headentry are positively correlated with the vibration velocity. Finally, measures for MSTCB prevention were proposed. The findings presented in this study can provide guidance for the prevention and control of MSTCBs in SIETCSs.
Late Paleozoic tectonics of Southern Central Asian orogenic belt: Evidence from magmatic rocks in the northern Alxa, Northwest China
Authors: Wang Er-Teng , Zhai Xin-Wei , Chen Wan-Feng , Ma Zhen , Wu Lei , Guo Zhi-Ang , Wang Yun , Song Gao-Rui , Wang Jin-Rong
Published: January 2023

Late Paleozoic magmatic rock outcrops are common in the Northern Alxa, Southern Central Asian Orogenic Belt (CAOB), which is a key area for understanding tectonic processes and defining the final closure time of the Paleo-Asian Ocean (PAO). We present zircon U‒Pb chronology and whole-rock geochemistry data for late Paleozoic magmatic rocks from the Yagan area of northern Alxa. This paper reveals two periods of magmatism: syenogranite (374.8 Ma) and bimodal intrusive rocks, which consist of gabbro (261.4 Ma), diabase (262.9 Ma) and biotite monzogranite (263.4 Ma). The syenogranite is high-K calc-alkaline and shows enrichments in Th, Zr, Hf and LREEs; depletions in Sr, Nb, Ta, and Ti; and low Mg# values (6.9–13.2); the syenogranite was derived from partial melting of the crust and has volcanic arc characteristics. The gabbro and diabase have similar geochemical characteristics, such as enrichments in Pb, Rb, Sr, Zr, and Hf and depletions in Nb, Ta, and Ti, with positive εHf(t) values (+0.9–+2.7 and +2.6–+3.6, respectively), indicating that they originated from partial melting of depleted mantle and experienced crustal contamination during magma emplacement. The biotite monzogranite shows depletions in Nb, Ta, and Ti and εNd(t) values of -2.6 to −2.4 and resulted from partial melting of the lower crust caused by asthenospheric underplating. The bimodal intrusive rocks formed in a postcollision extensional setting. Combined with previous data, we conclude that northern Alxa was an active continental margin during the late Devonian and that the final closure of the Yagan branch ocean of the PAO occurred prior to the middle Permian.
Spatial-temporal evolution of influencing mechanism of urban flooding in the Guangdong Hong Kong Macao greater bay area, China
Authors: Zhang Chunbo , Xu Tao , Wang Teng , Zhao Yaolong
Published: January 2023

Extreme weather has been more frequent in recent years. Urban agglomerations, as areas with a high density of human activities, have been plagued by storm flooding. Historically, the main focus of attention on flood control in urban agglomerations has gradually shifted from underground pipe networks to the impervious surface, reflecting profound changes in the influencing mechanism of urban flooding. Exploring the evolution of the mechanisms influencing urban flooding in the Guangdong Hong Kong Macao Greater Bay Area (GBA) urban agglomeration is of great reference significance for formulating flood prevention and control measures and promoting high-quality development of the GBA city cluster. In this paper, we fully use the collected information on urban flooding events from 1980 to 2018 in the GBA city cluster. Correlation analysis and geographically weighted regression (GWR) are used to analyze the influence of impervious surface percentage (ISP), impervious surface aggregation index (AI), impervious surface mean shape index (Shape_MN), vegetation cover (FVC), water surface ratio (WSR), relative elevation (RE) and slope on flooding in urban clusters and their evolution characteristics over time from a global perspective and spatial heterogeneity, respectively. The results show that: 1) ISP, AI, Shape_MN, and WSR are positively correlated with urban flooding, while FVC, RE, and Slope are negatively correlated with urban flooding. The correlations of each factor showed a general trend of gradual strengthening over time, and the increase rate slowed down after 2000, while the correlation of WSR showed a relatively noticeable decrease. 2) The GWR results show that each factor’s influence on urban flooding has pronounced spatial-temporal heterogeneity, and each factor shows different distribution characteristics. This study uses long time series of urban flooding point data to explore the spatial-temporal evolution of the influencing mechanism of urban flooding in the GBA urban agglomeration. We hope to provide a scientific basis for an in-depth understanding of the causes of urban flooding in the GBA, intending to provide auxiliary decision-making support for the formulation of waterlogging prevention and control measures.
Andesites and evolution of the continental crust: Perspectives from the Central Volcanic Zone of the Andes
Authors: Burns Dale H. , de Silva Shanaka L.
Published: January 2023

Named for the Andes, andesites (53%–63% SiO2) are the archetypal magma erupted at magmatic arcs. They have been established as the average composition of continental crust and as such are integral to the growth and evolution of the continental crust. However, andesites are quite variable in trace element and isotopic composition reflecting disparate paths of origin. Herein we return to the original site of their identification, the Central Andes, and use a comprehensive dataset of published and unpublished trace elements and isotopes to show that during the past 6 Myr two distinct types of andesite have erupted in the Central Volcanic Zone (CVZ), which correspond with different geodynamic conditions. Consistent with previous work, we confirm that major composite cones and minor centers of the steady state (low magmatic flux) Quaternary CVZ arc have trace element and isotopic characteristics consistent with magma generation/fractionation in the lower crust. Within the Quaternary arc centers, there are also significant latitudinal variations that correspond with the age, composition, and P-T conditions of the lower crust. However, in contrast to this prevailing model, in the 21–24°S segment 6–1 Ma andesites from ignimbrites and lava domes associated with the peak of the regional Neogene ignimbrite flare-up have compositions that indicate these andesites are hybrids between mantle-derived basalts and upper crustal lithologies. Since ∼1 Ma, andesites in young silicic lava domes associated with the regional flare-up are compositionally indistinguishable from proximal Quaternary arc centers, indicating a return to steady-state magmatism and lower crustal production of andesites. We propose that the transition from upper crustal to lower crustal andesite production results from a decrease in mantle heat input and subsequent relaxation of the regional geotherm during the waning of the flare-up event. The two modes of andesite production have significant implications for the production and evolution of the CVZ arc crust. During the flare-up, prodigious amounts of basalt were emplaced into the mid-crust, resulting in the production of large volumes of hybrid intermediate magmas in the mid and upper crust. In contrast, the lower crustal differentiation recorded in the Quaternary steady state arc andesites would result in the formation of a dense crystalline residue in the lower crust and an overall densification of the lower crust. Over time, gravity instabilities associated with this densification may ultimately aid in the delamination of the dense lower crustal root, triggering flare-ups. These differences in andesite production may help explain the cyclicity (flare-up cycles) observed in mature continental arcs and emphasizes that andesite is not a monotonous composition and can vary with depth-dependent intra-crustal differentiation related to magmatic flux.
Seismic-wave path attenuation and local site responses in eastern Guangdong province revealed by the ground-motion spectral analyses
Authors: Wu Huadeng , Zhou Ying , Qian Yongmei , Yang Fang , Yu Gang , Wu Guorui , Zhang Yi
Published: January 2023

In an effort to investigate the attenuation characteristics and site effects in eastern Guangdong, 659 strong-motion recordings received by 27 stations were selected for spectral analysis to separate path attenuation, site effects and source spectra. The path attenuation curves are generally close to the simplest form of distance decay described by R−0.5, and decays the slowest at a frequency of 4 Hz. We decomposed the path attenuation into a combination of geometric spreading and inelastic attenuation. The geometric spreading R−0.15 exhibits a slower distance decay at local distances, indicating that the seismic energy close to the epicenter is not easily dissipated. The quality factors were regressed as Qs(f) = 114.81f.922 in the frequency range .9–20 Hz, lower than those reported for the whole Guangdong region, indicating stronger anelastic attenuation in eastern Guangdong. The site responses in eastern Guangdong show a strong correlation with geological and geotechnical characterization. The stations located in the central mountains have higher predominant frequencies (>10 Hz) accompanied by smaller site amplifications. Larger site amplification is in the southeast coastal area (i.e., Shantou area), where the surface is covered with a thicker layer of soft soil and the predominant frequency of site is relatively low (∼2 Hz). The far-field earthquake will cause greater vibration and damage to this area.
Anesthesiology education: A discussion on the current reform for training in the hypoxic environment of the highlands of China
Authors: Chen Hongyang , Wang Zining , Zheng Lan , Ciren Jiumei , Zhang Weiyi , Zhu Tao
Published: January 2023

Highlights

Anesthesiology education in Tibet is lagging behind, and clinical anesthesia needs to be improved.

To improve the overall quality of anesthesiology teachers in Tibet is beneficial to the development of local anesthesiology.

Improving the teaching ability of anesthesiologists not only promotes the improvement of the independent learning ability of young doctors, but also lays a solid foundation for the smooth development of clinical work.
Identification and analysis of lncRNA, miRNA and mRNA related to subcutaneous and intramuscular fat in Laiwu pigs
Authors: Feng Hui , Liu Tianyi , Yousuf Salsabeel , Zhang Xiuxiu , Huang Wanlong , Li Ai , Xie Lingli , Miao Xiangyang
Published: January 2023

Background

Long non-coding RNAs (lncRNAs) regulate adipocyte differentiation and metabolism, However, their function on subcutaneous and intramuscular adipose tissues in pigs is unclear. Intramuscular fat (IMF) is an important indicator for evaluating meat quality. Breeds with high IMF content are often accompanied by high subcutaneous fat (SCF), which severely affects the meat rate of pigs. It is of great significance for porcine breeding to study the mechanism of lncRNA related to adipogenesis and lipid metabolism.

Methods

We identified differentially expressed lncRNAs, miRNAs and mRNAs in subcutaneous and intramuscular adipose tissues in three female Laiwu pigs by deep RNA-sequencing(|log2foldchange|≥1, P_value ≤ 0.05). The gene expression profiles of IMF and SCF in Laiwu pigs were comparatively analyzed by Bioinformatics methods to identify key lncRNAs, miRNAs, and mRNAs associated with lipid metabolism and adipogenesis.

Results

A total of 1209 lncRNAs (DElncRNAs), 286 miRNAs (DEmiRNAs), and 1597 mRNAs (DEgenes) were differentially expressed between two types of adipose. Among them, 17 DElncRNAs and 103 target genes play a role in the co-expression network, as well as 59 DElncRNAs, 44 DEmiRNAs, and 88 DEgenes involved in ceRNA network. In GO(Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis of DElncRNAs their target genes involved in many adipogenesis and lipid metabolism biological processes and signaling pathways, such as PPAR signaling pathway, Wnt signaling pathway, MAPK signaling pathway.

Conclusions

By constructing co-expression and ceRNAs network we found that Wnt signaling pathway play a critical regulatory role in intramuscular adipogenesis and lipid accumulation in Laiwu pigs. TCONS_00006525, TCONS_00046551 and TCONS_00000528 may target WNT5A, WNT10B and FDZ3 in co-expression network, TCONS_00026517 and other lncRNAs regulate the expression of PPARG, RXRG and SCD in ceRNA network, and were involved in Wnt signaling pathway. This study provides a theoretical basis for further understanding the post-transcriptional regulation mechanism of meat quality formation, predicting and treating diseases caused by ectopic fat.
Probiotic induced synthesis of microbiota polyamine as a nutraceutical for metabolic syndrome and obesity-related type 2 diabetes
Authors: Bui Tina I. , Britt Emily A. , Muthukrishnan Gowrishankar , Gill Steven R.
Published: January 2023

The gut microbiota regulates multiple facets of host metabolism and immunity through the production of signaling metabolites, such as polyamines which are small organic compounds that are essential to host cell growth and lymphocyte activation. Polyamines are most abundant in the intestinal lumen, where their synthesis by the gut microbiota is influenced by microbiome composition and host diet. Disruption of the host gut microbiome in metabolic syndrome and obesity-related type 2 diabetes (obesity/T2D) results in potential dysregulation of polyamine synthesis. A growing body of evidence suggests that restoration of the dysbiotic gut microbiota and polyamine synthesis is effective in ameliorating metabolic syndrome and strengthening the impaired immune responses of obesity/T2D. In this review, we discuss existing studies on gut microbiome determinants of polyamine synthesis, polyamine production in obesity/T2D, and evidence that demonstrates the potential of polyamines as a nutraceutical in obesity/T2D hosts.
Research on key cobalt technologies based on patent analysis
Authors: Hua Zhijing , Dai Tao
Published: January 2023

Based on global cobalt technology patents, this paper uses patent data mining and co-occurrence methods to identify technology application trends, key technology areas, key technology layouts, and technology roadmap. According to the development trend, the development of co-cobalt technology is divided into slow and rapid development stages. The conclusion are: 1) In terms of key technologies, E35-V (Cobalt compound) is the long-term R&D focus in the field of cobalt, and X16-B01F1 (Lithium-based) is the most important key technology area at present. 2) China, Japan, the United States, and South Korea are the main contributors to key cobalt technologies, mainly focusing on the R&D layout of L03-E01B5B (Lithium electrode) technology in the field of X16-B01F1 (Lithium-based). 3) Regarding the cooperation relationship between patent applicants, cooperation between groups is relatively close, cooperation between different countries is less, and transnational cooperation is mainly concentrated in developed countries, among which the United States has the most transnational partners. 4) In the field of cobalt’s most important electrode material technology LG Chemical Co., Ltd. (Korea) and Sumitomo Metal Mining Corporation (Japan) are the two companies with clear technology lines, while Univ Cent South (China) has a late technology development. Finally, we make suggestions for the development of cobalt technology: focus on core technology for R&D and innovation, actively study the technology status and innovation mode of leading companies, and strengthen the cooperation among different R&D institutions.
Structural optimization and simulation of piezoelectric- piezoresistive coupled MEMS steady-state electric field sensor
Authors: Liu Guote , Ye Yuanhao , Luo Bing , Gu Yu , Zheng Weijia , Chen Sijun
Published: January 2023

Abstract: In view of the problems of large volume, high energy consumption and difficult maintenance of electric field measurement sensors in existing power systems, non-contact miniature electric field sensors have become a hot topic in current research. In this paper, a MEMS miniature electric field measurement sensor model based on the principle of piezoelectric-piezoresistive coupling is constructed, and the sensor structure is optimized by analyzing the steady-state characteristics of the piezoelectric material and semiconductor membrane of the sensor. The input and output characteristics of the sensor were tested. The test results show that the sensor has excellent mechanical strain capacity, and the output voltage of the sensor has a linear relationship with the electric field strength, thus verifying the feasibility of the sensor measurement in the electric field. The research results will provide some reference for the development of contactless coupled sensors.
Indexing energy and cost of the pretreatment for economically efficient bioenergy generation
Authors: Preethi  , M Gunasekaran , Banu. J Rajesh
Published: January 2023

The growing necessity for energy worldwide has led to the hunt for an interminable solution in the form of sustainable energy generation. To accomplish sustainability, these problems can be resolved using renewable waste biomass, which is readily accessible and low priced. Moreover, the ecological issue due to the disposal of this waste biomass into the environment is also counteracted by the use of this biomass for energy generation along with the substantial solid reduction for disposal. The presence of complex biopolymers in biomass, which hasten the hydrolysis step during energy generation, was enhanced by the application of a pretreatment method. The efficiency of the pretreatment methods was enhanced by maintaining the cost and energy usage since the commercialization of this method is largely limited. The major economic drivers are based on solid concentration and, thus, lead to higher capital costs. This study reveals the wide assortment of current progression in pretreatment techniques for treating waste biomass with special focus on combined and phase-separated pretreatment. Additionally, it converses the advantages and limitations of pretreatment methods. This pivotal investigation brings about the cost- and energy-effective conversion solution that paves the way for a sustainable energy system.
Neutronics and thermal-hydraulics coupling analysis for a small lead-based fast reactor based on the discrete ordinate nodal and parallel channel method
Authors: Fang Chao , Peng Xingjie , Zhao Chen , Lou Lei , Zhang Bin , Wang Lianjie , Chen Zhang , Li Qing
Published: January 2023

The full-core neutronics/thermal-hydraulics (NE/TH) coupling analysis for a small lead-based fast reactor (SLFR) was presented. The NE-to-TH coupling occurs via power distribution, whereas the TH-to-NE coupling occurs via the material cross section, which is related to the temperature distribution. In the NE module, a discrete ordinate (SN) nodal solver is implemented based on the hexagonal prism mesh. In the TH module, a parallel channel model is implemented with each hexagonal fuel assembly as a channel. The mass flow of each channel is searched according to the same pressure drop, the temperature and density of the coolant within each channel is calculated with a one-dimensional axial heat convection model, and the temperature distribution along the fuel pin is calculated with a one-dimensional radial heat conduction model. In order to update the cross section, a temperature-dependent homogenized microscopic cross-section library is prepared in advance, and based on the temperature distribution obtained from the TH calculation, the cross-section data required by the NE calculation are obtained by interpolation. The results of the NE/TH coupling analysis for SLFR indicate that the TH parameters (the maximum temperature of the fuel, cladding, and coolant and the maximum velocity of the coolant) are all within the design limits, and the TH feedback provides negative reactivity feedback (about −200 pcm) and has a fairly small effect on the power distribution (<1.0%).
Reduction in energy consumption and CO2 emissions by retrofitting an existing building to a net zero energy building for the implementation of SDGs 7 and 13
Authors: Razzaq Ibtisam , Amjad Muhammad , Qamar Adnan , Asim Muhammad , Ishfaq Kashif , Razzaq Afia , Mawra Khadija
Published: January 2023

This work aims to reduce the energy consumption of existing buildings through retrofitting and converting them into net zero energy buildings (NZEBs). Various retrofits for an existing academic building were modeled and analyzed using REVIT software. Energy consumption was reduced to approximately 34% through retrofits, and a Solar System of 41.6 kW was integrated to meet the reduced energy demand, consequently converting the building into an net zero energy building (NZEB). Furthermore, environmental and economic analyses were conducted, and the results show a reduction of 32.8 metric tons of CO2 emissions per year as a result of retrofitting the building, converting it into an net zero energy building. The building envelope retrofits and electrical appliances have a payback period of 2.96 and 2.62 years, respectively, whereas the proposed Solar System has a payback period of only 2.3 years. Moreover, the building was rated using the Leadership in Energy and Environmental Design (LEED) rating tool, and it qualifies for silver certification after retrofits and the integration of renewable energy sources (RES). The reduction in energy consumption and integration of renewable energy sources contribute to achieving Sustainable Development Goal (SDG) 7, and reduced CO2 emissions save climate variations, which leads to achieving Sustainable Development Goal 13.
On the importance of invasive species niche dynamics in plant conservation management at large and local scale
Authors: Santamarina Sara , Mateo Rubén G. , Alfaro-Saiz Estrella , Acedo Carmen
Published: January 2023

Predicting the distribution of Invasive alien species (IAS) using species distribution models is promising for conservation planning. To achieve accurate predictions, it is essential to explore species niche dynamics. New approaches are necessary for bringing this analysis to real conservation management needs. Using multi-site comparisons can provide great useful insights to better understand invasion processes. Exploring the fine-scale niche overlap between IAS and native species sharing a location can be a key tool for achieving the implementation of local species conservation actions, which can play a fundamental role in the global management of IAS. This can also increase society’s awareness of the threat of IAS. In this context, here, we explored two key research demands. First, we studied the large-scale niche dynamics of the invasive species Paraserianthes lophantha (Willd.) I.C. Nielsen’s considering different invaded areas. The analysis compared niches of the native range (South Western Australia) with the Australian invaded range (eastern Australia); the native range with the European invaded range, and its full Australian range (native plus invaded range) with the European invaded range. Second, we perform a fine-scale niche overlap analysis at landscape scale in Spain. We studied the niche overlap between P. lophantha and a species with remarkable conservation interest (Quercus lusitanica Lam). All the niche analyses were realized following a well-established ordination (principal component analysis) approach where important methodological aspects were compared and analyzed. Our multi-site study of P. lophantha large-scale niche dynamics detected niche shifts between the Australian ranges demonstrating that the species is labile and may potentially adapt to further European climate conditions and spread its invasive range. Comparative analysis between the European and the full Australian ranges supports that calibrate models including the Australian invasive information is promising to accurate predict P. lophantha European potential distribution. The fine-scale study of niche overlap further explained the potential of this IAS and can be used as a model example of how these local studies can be used to promote the implementation of conservation actions in situ as a complement to large-scale management strategies.
Species diversity, hotspot congruence, and conservation of North American damselflies (Odonata: Zygoptera)
Authors: Zhao Zhengxue , Feng Xueli , Zhang Yubo , Wang Yingjian , Zhou Zhengxiang
Published: January 2023

The rapid extinction of species is of considerable concern for biodiversity conservation. Identifying the drivers of species diversity and hotspots is beneficial for developing conservation strategies. Studies on insects have mainly focused on terrestrial species and rarely on semiaquatic species. Using 135,208 georeferenced occurrence records of 296 damselflies across North America, their species richness and endemism (represented by weighted endemism) patterns were mapped in a 100 × 100-km grid size, and the effects of environmental variables on species richness and endemism were investigated using generalized linear models and hierarchical partitioning. Subsequently, the top 5% grids with species richness and weighted endemism were separately selected as hotspots and their congruence was evaluated. Finally, species diversity hotspots were identified by integrating two types of hotspot grids, and gap analysis was performed to evaluate their conservation status. Temperature conditions and water availability had the strongest influence on species richness and endemism, respectively. Low congruence among species richness and endemism hotspots was observed. Moreover, four species diversity hotspots were identified, namely, region of the eastern United States and southeastern Canada, southwestern United States, central Mexico, and southernmost North America. Approximately 69.31% of the hotspot grids are not a part of the existing protected areas, presenting a significant conservation gap. The habitats of taxonomic groups should be considered while identifying the most common driving mechanisms of endemism. Strengthening the establishment of protected areas in regions with conservation gaps is urgently needed to promote the conservation of damselflies in North America.
The impact of the Asian chestnut gall wasp (Dryocosmus kuriphilus) on chestnut tree growth may be mediated by site resources
Authors: Castedo-Dorado Fernando , Álvarez-Álvarez Pedro , Lombardero María Josefa
Published: January 2023

Introduction

The Asian chestnut gall wasp (Dryocosmus kuriphilus) is a major pest of chestnut trees worldwide, seriously affecting chestnut cultivation. Information concerning the effects of gall wasp attack on diameter growth of chestnut trees is currently scarce and limited to coppice stands and to use of the growth of a non-target control species (unaffected by the pest) for reference purposes. The effects of the pest on widely-spaced plantations (grown at a much lower density than chestnut coppices) and the use of explicitly-observed annual infestation rate data remain to be explored.

Methods

In the present study, we analyzed the impact of the chestnut gall wasp on the diameter growth of chestnut trees, using data from 16 experimental plots established in widely-spaced plantations located in good quality sites. Two of the plots are in plantations where a susceptible hybrid chestnut clone and a chestnut clone resistant to the gall wasp coexist, whereas the remaining 14 plots are in Castanea sativa plantations where the level of gall wasp infestation varies across trees and years. The plots were surveyed to determine the diameter growth of the trees and the level of infestation during 5 years (2017–2021).

Results

The infestation level corresponding to the theoretical damage threshold was surpassed inmost plots during the study period. Nevertheless, there were no differences in the growth of attacked and unaffected plants in the two plots planted with hybrid clones with contrasting susceptibility to the gall wasp. The attack had a modest effect in C. sativa plots, with a mean reduction in annual basal area increment of 9.9%.

Discussion

These findings apparently contradict previous reports of a marked reduction in radial growth of chestnut coppice trees due to gall wasp attack. The difference in findings may be related to increased compensation for herbivory with increasing levels of resources (especially light) in the plantations under study, which were less dense than previously studied stands. The study outcomes add to existing knowledge on the impact of chestnut gall wasp on wood formation and may have implications regarding planting site recommendations and subsequent stand management.
Infertility control of transgenic fluorescent zebrafish with targeted mutagenesis of the dnd1 gene by CRISPR/Cas9 genome editing
Authors: Chu Wai-Kwan , Huang Shih-Chin , Chang Ching-Fong , Wu Jen-Leih , Gong Hong-Yi
Published: January 2023

Transgenic technology and selective breeding have great potential for the genetic breeding in both edible fish and ornamental fish. The development of infertility control technologies in transgenic fish and farmed fish is the critical issue to prevent the gene flow with wild relatives. In this study, we report the genome editing of the dead end (dnd1) gene in the zebrafish model, using the CRISPR/Cas9 technology to achieve a loss-of-function mutation in both wild-type zebrafish and transgenic fluorescent zebrafish to develop complete infertility control technology of farmed fish and transgenic fish. We effectively performed targeted mutagenesis in the dnd1 gene of zebrafish with a single gRNA, which resulted in a small deletion (−7 bp) or insertion (+41 bp) in exon 2, leading to a null mutation. Heterozygotes and homozygotes of dnd1-knockout zebrafish were both selected by genotyping in the F1 and F2 generations. Based on a comparison of histological sections of the gonads between wild-type, heterozygous, and homozygous dnd1 zebrafish mutants, the dnd1 homozygous mutation (aa) resulted in the loss of germ cells. Still, there was no difference between the wild-type (AA) and dnd1 heterozygous (Aa) zebrafish. The homozygous dnd1 mutants of adult zebrafish and transgenic fluorescent zebrafish became all male, which had normal courtship behavior to induce wild-type female zebrafish spawning. However, they both had no sperm to fertilize the spawned eggs from wild-type females. Thus, all the unfertilized eggs died within 10 h. The targeted mutagenesis of the dnd1 gene using the CRISPR/Cas9 technology is stably heritable by crossing of fertile heterozygous mutants to obtain sterile homozygous mutants. It can be applied in the infertility control of transgenic fluorescent fish and genetically improved farmed fish by selective breeding to promote ecologically responsible aquaculture.
Phenotypic and genetic characteristics of 130 patients with mucopolysaccharidosis type II: A single-center retrospective study in China
Authors: Zhang Zhenjie , Ma Mingsheng , Zhang Weimin , Zhou Yu , Yao Fengxia , Zhu Lisi , Wei Min , Qiu Zhengqing
Published: January 2023

Background: Mucopolysaccharidosis Type II (MPS II) is a rare, progressive and ultimately fatal X-linked lysosomal storage disorder caused by mutations in the iduronate-2-sulfatase (IDS) gene. This report conducted a retrospective analysis to investigate the clinical characteristics, genotypes and management strategies in a large cohort of Chinese patients with MPS II.

Methods: In this study, we explored 130 Chinese patients with MPS II between September 2008 and April 2022. Clinical manifestations, auxiliary examination, IDS pathogenic gene variants and IDS enzyme activity, surgical history were analysed in the study.

Results: A total of 130 patients were enrolled and the mean age at diagnosis was 5 years old. This study found the most common symptoms in our patients were claw-like hands, followed by coarse facial features, birthmarks (Mongolian spot), delayed development, inguinal or umbilical hernia. The most commonly cardiac manifestations were valve abnormalities, which were mitral/tricuspid valve regurgitation (71.9%) and aortic/pulmonary valve regurgitation (36.8%). We had found 43 different IDS pathogenic gene variants in 55 patients, included 16 novel variants. The variants were concentrated in exon 9 (20% = 11/55), exon 3 (20% = 11/55) and exon 8 (15% = 8/55). A total of 50 patients (38.5%) underwent surgical treatment, receiving a total of 63 surgeries. The average age of first surgery was 2.6 years, and the majority of surgery (85.7%, 54/63) was operated before 4 years old. The most common and earliest surgery was hernia repair. Three patients were died of respiratory failure.

Conclusion: This study provided additional information on the clinical, cardiac ultrasound and surgical procedure in MPS II patients. Our study expanded the genotype spectrum of MPS II. Based on these data, characterization of MPS II patients group could be used to early diagnosis and treatment of the disease.
Genome-wide assessment of the population structure and genetic diversity of four Portuguese native sheep breeds
Authors: Gaspar Daniel , Usié Ana , Leão Célia , Guimarães Sílvia , Pires Ana Elisabete , Matos Claudino , Ramos António Marcos , Ginja Catarina
Published: January 2023

As the effects of global warming become increasingly complex and difficult to manage, the conservation and sustainable use of locally adapted sheep breeds are gaining ground. Portuguese native sheep breeds are important reservoirs of genetic diversity, highly adapted to harsh environments and reared in low input production systems. Genomic data that would describe the breeds in detail and accelerate the selection of more resilient animals to be able to cope with climatic challenges are still lacking. Here, we sequenced the genomes of 37 animals from four Portuguese native sheep breeds (Campaniça, Bordaleira Serra da Estrela, Merino Branco and Merino Preto) and 19 crossbred sheep to make inferences on their genomic diversity and population structure. Mean genomic diversities were very similar across these breeds (.30 ≤ Ho ≤ .34; .30 ≤ He ≤ .35; 1.7 × 10–3 ≤ π ≤ 3.1 × 10–3) and the levels of inbreeding were negligible (.005 ≤ FIS ≤ .038). The Principal Components, Bayesian clustering and Treemix analyses split the Portuguese breeds in two main groups which are consistent with historical records: one comprising Campaniça and Serra da Estrela together with other European and transboundary dairy breeds; and another of the well-differentiated multi-purpose Merino and Merino-related breeds. Runs of homozygosity analyses yielded 1,690 ROH segments covering an average of 2.27 Gb across the genome in all individuals. The overall genome covered by ROH segments varied from 27,75 Mb in Serra da Estrela to 61,29 Mb in Campaniça. The phylogenetic analysis of sheep mitogenomes grouped the Portuguese native breeds within sub-haplogroup B1a along with two animals of the Akkaraman breed from Turkey. This result provides additional support to a direct influence of Southwest Asian sheep in local breeds from the Iberian Peninsula. Our study is a first step pertaining to the genomic characterization of Portuguese sheep breeds and the results emphasize the potential of genomic data as a valid tool to guide conservation efforts in locally adapted sheep breeds. In addition, the genomic data we generated can be used to identify markers for breed assignment and traceability of certified breed-products.
Effect of GnRH immunocastration on immune function in male rats
Authors: Pan Fuqiang , Du Huiting , Tian Weiguo , Xie Huihui , Zhang Bochao , Fu Wanzhen , Li Yunsheng , Ling Yinghui , Zhang Yunhai , Fang Fugui , Liu Ya
Published: January 2023

The present study aimed to reveal the effects of immunocastration on the development of the immune system in rats. Seventy rats were randomly assigned into two groups: Control (n = 35) and immunized (n = 35). Twenty-day-old rats were immunized with gonadotropin-releasing hormone (GnRH) and booster immunization was administered every two weeks (three immunizations in total). From 20-day-old rats, we collected samples every two weeks, including five immunized rats and five control rats (seven collections in total). We collected blood samples, testicles, thymuses, and spleens. The results showed that GnRH immunization increased the GnRH antibody titers and reduced the testosterone concentration (both P < 0.05). Compared with the control group, the number of CD4+CD8− cells, CD4−CD8+ cells, and CD4+CD8+ cells increased (P < 0.05) whereas the number of CD4-CD8- cells and CD4+CD25+ cells reduced in the immunized group (P < 0.05) over time. GnRH immunization also increased the relative weights of thymus and spleen (P < 0.05), serum concentrations of interleukin (IL)-2, IL-4, IL-6, IL-10, IL-17 and Interferon-γ (IFN-γ) over time (P < 0.05), and changed the mRNA levels of IL-2, IL-4, IL-6. IL-10, IL-17, IFN-γ, CD4, D8, CD19 GnRH, and GnRH receptor (GnRH-R) in thymus and spleen. Thus, GnRH immunization enhanced the immune markers in thymus, spleen, and blood immune cytokines in rats.
Inhibition of complement activation by CD55 overexpression in human induced pluripotent stem cell derived kidney organoids
Authors: Gaykema Lonneke H. , van Nieuwland Rianne Y. , Dekkers Mette C. , van Essen Mieke F. , Heidt Sebastiaan , Zaldumbide Arnaud , van den Berg Cathelijne W. , Rabelink Ton J. , van Kooten Cees
Published: January 2023

End stage renal disease is an increasing problem worldwide driven by aging of the population and increased prevalence of metabolic disorders and cardiovascular disease. Currently, kidney transplantation is the only curative option, but donor organ shortages greatly limit its application. Regenerative medicine has the potential to solve the shortage by using stem cells to grow the desired tissues, like kidney tissue. Immune rejection poses a great threat towards the implementation of stem cell derived tissues and various strategies have been explored to limit the immune response towards these tissues. However, these studies are limited by targeting mainly T cell mediated immune rejection while the rejection process also involves innate and humoral immunity. In this study we investigate whether inhibition of the complement system in human induced pluripotent stem cells (iPSC) could provide protection from such immune injury. To this end we created knock-in iPSC lines of the membrane bound complement inhibitor CD55 to create a transplant-specific protection towards complement activation. CD55 inhibits the central driver of the complement cascade, C3 convertase, and we show that overexpression is able to decrease complement activation on both iPSCs as well as differentiated kidney organoids upon stimulation with anti-HLA antibodies to mimic the mechanism of humoral rejection.
The non-oral infection of larval Echinococcus granulosus induces immune and metabolic reprogramming in the colon of mice
Authors: Zhou Yuying , Luo Tiancheng , Gong Yuying , Guo Yuxin , Wang Dingmin , Gao Zixuan , Sun Fenfen , Fu Linlin , Liu Hua , Pan Wei , Yang Xiaoying
Published: January 2023

Background

The intestinal tract serves as a critical regulator for nutrient absorption and overall health. However, its involvement in anti-parasitic infection and immunity has been largely neglected, especially when a parasite is not transmitted orally. The present study investigated the colonic histopathology and functional reprogramming in mice with intraperitoneal infection of the larval Echinococcus granulosus (E. granulosus).

Results

Compared with the control group, the E. granulosus–infected mice exhibited deteriorated secreted mucus, shortened length, decreased expression of tight junction proteins zonula occludens-1 (ZO-1), and occludin in the colon. Moreover, RNA sequencing was employed to characterize colonic gene expression after infection. In total, 3,019 differentially expressed genes (1,346 upregulated and 1,673 downregulated genes) were identified in the colon of infected mice. KEGG pathway and GO enrichment analysis revealed that differentially expressed genes involved in intestinal immune responses, infectious disease-associated pathways, metabolism, or focal adhesion were significantly enriched. Among these, 18 tight junction-relative genes, 44 immune response-associated genes, and 23 metabolic genes were annotated. Furthermore, mebendazole treatment could reverse the colonic histopathology induced by E. granulosus infection.

Conclusions

Intraperitoneal infection with E. granulosus induced the pathological changes and functional reprogramming in the colon of mice, and mebendazole administration alleviated above alternations, highlighting the significance of the colon as a protective barrier against parasitic infection. The findings provide a novel perspective on host-parasite interplay and propose intestine as a possible target for treating parasitic diseases that are not transmitted orally.
ENSO coupling to the equatorial Atlantic: Analysis with an extended improved recharge oscillator model
Authors: Crespo-Miguel Rodrigo , Polo Irene , Mechoso Carlos R. , Rodríguez-Fonseca Belén , Cao-García Francisco J.
Published: January 2023

Introduction

Observational and modeling studies have examined the interactions between El Niño-Southern Oscillation (ENSO) and the equatorial Atlantic variability as incorporated into the classical charge-recharge oscillator model of ENSO. These studies included the role of the Atlantic in the predictability of ENSO but assumed stationarity in the relationships, i.e., that models’ coefficients do not change over time. A recent work by the authors has challenged the stationarity assumption in the ENSO framework but without considering the equatorial Atlantic influence on ENSO.

Methods

The present paper addresses the changing relationship between ENSO and the Atlantic El Niño using an extended version of the recharge oscillator model. The classical two-variable model of ENSO is extended by adding a linear coupling on the SST anomalies in the equatorial Atlantic. The model’s coefficients are computed for different periods. This calculation is done using two methods to fit the model to the data: (1) the traditional method (ReOsc), and (2) a novel method (ReOsc+) based on fitting the Fisher’s Z transform of the auto and cross-correlation functions.

Results

We show that, during the 20th century, the characteristic damping rate of the SST and thermocline depth anomalies in the Pacific have decreased in time by a factor of 2 and 3, respectively. Moreover, the damping time of the ENSO fluctuations has doubled from 10 to 20 months, and the oscillation period of ENSO has decreased from 60-70 months before the 1960s to 50 months afterward. These two changes have contributed to enhancing ENSO amplitude. The results also show that correlations between ENSO and the Atlantic SST strengthened after the 70s and the way in which the impact of the equatorial Atlantic is added to the internal ENSO variability.

Conclusions

The remote effects of the equatorial Atlantic on ENSO must be considered in studies of ENSO dynamics and predictability during specific time-periods. Our results provide further insight into the evolution of the ENSO dynamics and its coupling to the equatorial Atlantic, as well as an improved tool to study the coupling of climatic and ecological variables.
Feature preserving mesh network for semantic segmentation of retinal vasculature to support ophthalmic disease analysis
Authors: Imran Syed Muhammad Ali , Saleem Muhammad Waqas , Hameed Muhammad Talha , Hussain Abida , Naqvi Rizwan Ali , Lee Seung Won
Published: January 2023

Introduction

Ophthalmic diseases are approaching an alarming count across the globe. Typically, ophthalmologists depend on manual methods for the analysis of different ophthalmic diseases such as glaucoma, Sickle cell retinopathy (SCR), diabetic retinopathy, and hypertensive retinopathy. All these manual assessments are not reliable, time-consuming, tedious, and prone to error. Therefore, automatic methods are desirable to replace conventional approaches. The accuracy of this segmentation of these vessels using automated approaches directly depends on the quality of fundus images. Retinal vessels are assumed as a potential biomarker for the diagnosis of many ophthalmic diseases. Mostly newly developed ophthalmic diseases contain minor changes in vasculature which is a critical job for the early detection and analysis of disease.

Method

Several artificial intelligence-based methods suggested intelligent solutions for automated retinal vessel detection. However, existing methods exhibited significant limitations in segmentation performance, complexity, and computational efficiency. Specifically, most of the existing methods failed in detecting small vessels owing to vanishing gradient problems. To overcome the stated problems, an intelligence-based automated shallow network with high performance and low cost is designed named Feature Preserving Mesh Network (FPM-Net) for the accurate segmentation of retinal vessels. FPM-Net employs a feature-preserving block that preserves the spatial features and helps in maintaining a better segmentation performance. Similarly, FPM-Net architecture uses a series of feature concatenation that also boosts the overall segmentation performance. Finally, preserved features, low-level input image information, and up-sampled spatial features are aggregated at the final concatenation stage for improved pixel prediction accuracy. The technique is reliable since it performs better on the DRIVE database, CHASE-DB1 database, and STARE dataset.

Results and discussion

Experimental outcomes confirm that FPM-Net outperforms state-of-the-art techniques with superior computational efficiency. In addition, presented results are achieved without using any preprocessing or postprocessing scheme. Our proposed method FPM-Net gives improvement results which can be observed with DRIVE datasets, it gives Se, Sp, and Acc as 0.8285, 0.98270, 0.92920, for CHASE-DB1 dataset 0.8219, 0.9840, 0.9728 and STARE datasets it produces 0.8618, 0.9819 and 0.9727 respectively. Which is a remarkable difference and enhancement as compared to the conventional methods using only 2.45 million trainable parameters.