bluuebunny/crossref_metadata_2025 huggingface dataset:
Isolation of High-Purity Betanin from Red Beet and Elucidation of Its Antioxidant Activity against Peroxynitrite: An in vitro Study
Authors: Sakihama Yasuko , Kato Takashi , Sawatdee Sopanat , Yakushi Yo , Asano Junichi , Hayashi Hiroyo , Goto Yuya , Hashimoto Makoto , Hashidoko Yasuyuki
Published: October 2023

Reactive oxygen species and reactive nitrogen species (RNS) are damaging for many biomolecules. Peroxynitrite (ONOO−) is the most toxic molecular species among RNS. Betalains are known to possess ONOO− scavenging ability. Betanin, a betalain isolated from red beet, possesses antioxidant, anti-inflammatory, and antitumor activities; however, detailed studies of this isolated pigment have not been conducted, owing to its instability under physiological conditions. This study aimed to isolate highly purified betanin from red beetroots using an improved purification method involving deproteinization and citric acid co-precipitation and evaluated its antioxidant activities. The purified betanin thus obtained had a significantly lower isobetanin content than the commercially available betanin dyes. The antioxidant activity of purified betanin examined in the 2,2-diphenyl-1-picrylhydrazyl assay, the direct ONOO− reaction, ONOO−-dependent DNA damage, and lipid peroxidation reactions revealed that betanin possessed higher antioxidant capacity than general antioxidants such as ascorbic acid and quercetin. Furthermore, betanin showed indirect and direct cytoprotective effects against H2O2 and ONOO− cytotoxicity, respectively, in cultured mouse fibroblasts. To the best of our knowledge, this is the first study to demonstrate the cytoprotective effects of betanin against ONOO− toxicity. The highly purified betanin obtained in this study will aid in further exploring its physiological functions.
Data-Independent Acquisition Mass Spectrometry Analysis of FFPE Rectal Cancer Samples Offers In-Depth Proteomics Characterization of the Response to Neoadjuvant Chemoradiotherapy
Authors: Stanojevic Aleksandra , Samiotaki Martina , Lygirou Vasiliki , Marinkovic Mladen , Nikolic Vladimir , Stojanovic-Rundic Suzana , Jankovic Radmila , Vlahou Antonia , Panayotou George , Fijneman Remond J. A. , Castellví-Bel Sergi , Zoidakis Jerome , Cavic Milena
Published: October 2023

Locally advanced rectal cancer (LARC) presents a challenge in identifying molecular markers linked to the response to neoadjuvant chemoradiotherapy (nCRT). This study aimed to utilize a sensitive proteomic method, data-independent mass spectrometry (DIA-MS), to extensively analyze the LARC proteome, seeking individuals with favorable initial responses suitable for a watch-and-wait approach. This research addresses the unmet need to understand the response to treatment, potentially guiding personalized strategies for LARC patients. Post-treatment assessment included MRI scans and proctoscopy. This research involved 97 LARC patients treated with intense chemoradiotherapy, comprising radiation and chemotherapy. Out of 97 LARC included in this study, we selected 20 samples with the most different responses to nCRT for proteome profiling (responders vs. non-responders). This proteomic approach shows extensive proteome coverage in LARC samples. The analysis identified a significant number of proteins compared to a prior study. A total of 915 proteins exhibited differential expression between the two groups, with certain signaling pathways associated with response mechanisms, while top candidates had good predictive potential. Proteins encoded by genes SMPDL3A, PCTP, LGMN, SYNJ2, NHLRC3, GLB1, and RAB43 showed high predictive potential of unfavorable treatment outcome, while RPA2, SARNP, PCBP2, SF3B2, HNRNPF, RBBP4, MAGOHB, DUT, ERG28, and BUB3 were good predictive biomarkers of favorable treatment outcome. The identified proteins and related biological processes provide promising insights that could enhance the management and care of LARC patients.
Role of Neuronal TRPC6 Channels in Synapse Development, Memory Formation and Animal Behavior
Authors: Zernov Nikita , Popugaeva Elena
Published: October 2023

The transient receptor potential cation channel, subfamily C, member 6 (TRPC6), has been believed to adjust the formation of an excitatory synapse. The positive regulation of TRPC6 engenders synapse enlargement and improved learning and memory in animal models. TRPC6 is involved in different synaptoprotective signaling pathways, including antagonism of N-methyl-D-aspartate receptor (NMDAR), activation of brain-derived neurotrophic factor (BDNF) and postsynaptic store-operated calcium entry. Positive regulation of TRPC6 channels has been repeatedly shown to be good for memory formation and storage. TRPC6 is mainly expressed in the hippocampus, particularly in the dentate granule cells, cornu Ammonis 3 (CA3) pyramidal cells and gamma-aminobutyric acid (GABA)ergic interneurons. It has been observed that TRPC6 agonists have a great influence on animal behavior including memory formation and storage The purpose of this review is to collect the available information on the role of TRPC6 in memory formation in various parts of the brain to understand how TRPC6-specific pharmaceutical agents will affect memory in distinct parts of the central nervous system (CNS).
Peptide-Decorated Degradable Polycarbonate Nanogels for Eliciting Antigen-Specific Immune Responses
Authors: Stickdorn Judith , Czysch Christian , Medina-Montano Carolina , Stein Lara , Xu Lujuan , Scherger Maximilian , Schild Hansjörg , Grabbe Stephan , Nuhn Lutz
Published: October 2023

For successful therapeutic interventions in cancer immunotherapy, strong antigen-specific immune responses are required. To this end, immunostimulating cues must be combined with antigens to simultaneously arrive at antigen-presenting cells and initiate cellular immune responses. Recently, imidazoquinolines have shown their vast potential as small molecular Toll-like receptor 7/8 (TLR7/8) agonists for immunostimulation when delivered by nanocarriers. At the same time, peptide antigens are promising antigen candidates but require combination with immune-stimulating adjuvants to boost their immunogenicity and exploit their full potential. Consequently, we herein present biodegradable polycarbonate nanogels as versatile delivery system for adjuvants within the particles’ core as well as for peptide antigens by surface decoration. For that purpose, orthogonally addressable multifunctional polycarbonate block copolymers were synthesized, enabling adjuvant conjugation through reactive ester chemistry and peptide decoration by strain-promoted alkyne-azide cycloaddition (SPAAC). In preparation for SPAAC, CD4+-specific peptide sequences of the model protein antigen ovalbumin were equipped with DBCO-moieties by site-selective modification at their N-terminal cysteine. With their azide groups exposed on their surface, the adjuvant-loaded nanogels were then efficiently decorated with DBCO-functional CD4+-peptides by SPAAC. In vitro evaluation of the adjuvant-loaded peptide-decorated gels then confirmed their strong immunostimulating properties as well as their high biocompatibility. Despite their covalent conjugation, the CD4+-peptide-decorated nanogels led to maturation of primary antigen-presenting cells and the downstream priming of CD4+-T cells. Subsequently, the peptide-decorated nanogels loaded with TLR7/8 agonist were successfully processed by antigen-presenting cells, enabling potent immune responses for future application in antigen-specific cancer immunotherapy.
Liquid–Liquid Phase Separation Sheds New Light upon Cardiovascular Diseases
Authors: Cai Ziyang , Mei Shuai , Zhou Li , Ma Xiaozhu , Wuyun Qidamugai , Yan Jiangtao , Ding Hu
Published: October 2023

Liquid–liquid phase separation (LLPS) is a biophysical process that mediates the precise and complex spatiotemporal coordination of cellular processes. Proteins and nucleic acids are compartmentalized into micron-scale membrane-less droplets via LLPS. These droplets, termed biomolecular condensates, are highly dynamic, have concentrated components, and perform specific functions. Biomolecular condensates have been observed to organize diverse key biological processes, including gene transcription, signal transduction, DNA damage repair, chromatin organization, and autophagy. The dysregulation of these biological activities owing to aberrant LLPS is important in cardiovascular diseases. This review provides a detailed overview of the regulation and functions of biomolecular condensates, provides a comprehensive depiction of LLPS in several common cardiovascular diseases, and discusses the revolutionary therapeutic perspective of modulating LLPS in cardiovascular diseases and new treatment strategies relevant to LLPS.
Renal Organic Anion Transporters 1 and 3 In Vitro: Gone but Not Forgotten
Authors: Caetano-Pinto Pedro , Stahl Simone H.
Published: October 2023

Organic anion transporters 1 and 3 (OAT1 and OAT3) play a crucial role in kidney function by regulating the secretion of multiple renally cleared small molecules and toxic metabolic by-products. Assessing the activity of these transporters is essential for drug development purposes as they can significantly impact drug disposition and safety. OAT1 and OAT3 are amongst the most abundant drug transporters expressed in human renal proximal tubules. However, their expression is lost when cells are isolated and cultured in vitro, which is a persistent issue across all human and animal renal proximal tubule cell models, including primary cells and cell lines. Although it is well known that the overall expression of drug transporters is affected in vitro, the underlying reasons for the loss of OAT1 and OAT3 are still not fully understood. Nonetheless, research into the regulatory mechanisms of these transporters has provided insights into the molecular pathways underlying their expression and activity. In this review, we explore the regulatory mechanisms that govern the expression and activity of OAT1 and OAT3 and investigate the physiological changes that proximal tubule cells undergo and that potentially result in the loss of these transporters. A better understanding of the regulation of these transporters could aid in the development of strategies, such as introducing microfluidic conditions or epigenetic modification inhibitors, to improve their expression and activity in vitro and to create more physiologically relevant models. Consequently, this will enable more accurate assessment for drug development and safety applications.
Endothelial Cell Dysfunction and Hypoxia as Potential Mediators of Pain in Fabry Disease: A Human-Murine Translational Approach
Authors: Klug Katharina , Spitzel Marlene , Hans Clara , Klein Alexandra , Schottmann Nicole Michelle , Erbacher Christoph , Üçeyler Nurcan
Published: October 2023

Fabry disease (FD) is caused by α-galactosidase A (AGAL) enzyme deficiency, leading to globotriaosylceramide accumulation (Gb3) in several cell types. Pain is one of the pathophysiologically incompletely understood symptoms in FD patients. Previous data suggest an involvement of hypoxia and mitochondriopathy in FD pain development at dorsal root ganglion (DRG) level. Using immunofluorescence and quantitative real-time polymerase chain reaction (qRT PCR), we investigated patient-derived endothelial cells (EC) and DRG tissue of the GLA knockout (KO) mouse model of FD. We address the question of whether hypoxia and mitochondriopathy contribute to FD pain pathophysiology. In EC of FD patients (P1 with pain and, P2 without pain), we found dysregulated protein expression of hypoxia-inducible factors (HIF) 1a and HIF2 compared to the control EC (p < 0.01). The protein expression of the HIF downstream target vascular endothelial growth factor A (VEGFA, p < 0.01) was reduced and tube formation was hampered in the P1 EC compared to the healthy EC (p < 0.05). Tube formation ability was rescued by applying transforming growth factor beta (TGFβ) inhibitor SB-431542. Additionally, we found dysregulated mitochondrial fusion/fission characteristics in the P1 and P2 EC (p < 0.01) and depolarized mitochondrial membrane potential in P2 compared to control EC (p < 0.05). Complementary to human data, we found upregulated hypoxia-associated genes in the DRG of old GLA KO mice compared to WT DRG (p < 0.01). At protein level, nuclear HIF1a was higher in the DRG neurons of old GLA KO mice compared to WT mice (p < 0.01). Further, the HIF1a downstream target CA9 was upregulated in the DRG of old GLA KO mice compared to WT DRG (p < 0.01). Similar to human EC, we found a reduction in the vascular characteristics in GLA KO DRG compared to WT (p < 0.05). We demonstrate increased hypoxia, impaired vascular properties, and mitochondrial dysfunction in human FD EC and complementarily at the GLA KO mouse DRG level. Our data support the hypothesis that hypoxia and mitochondriopathy in FD EC and GLA KO DRG may contribute to FD pain development.
Repression of GhTUBB1 Reduces Plant Height in Gossypium hirsutum
Authors: Zhang Lihua , Ma Caixia , Wang Lihua , Su Xiaofeng , Huang Jinling , Cheng Hongmei , Guo Huiming
Published: October 2023

The original ‘Green Revolution’ genes are associated with gibberellin deficiency. However, in some species, mutations in these genes cause pleiotropic phenotypes, preventing their application in dwarf breeding. The development of novel genotypes with reduced plant height will resolve this problem. In a previous study, we obtained two dwarf lines, L28 and L30, by introducing the Ammopiptanthus mongolicus (Maxim. ex Kom.) Cheng f. C-repeat-binding factor 1 (AmCBF1) into the upland cotton variety R15. We found that Gossypium hirsutum Tubulin beta-1 (GhTUBB1) was downregulated in L28 and L30, which suggested that this gene may have contributed to the dwarf phenotype of L28 and L30. Here, we tested this hypothesis by silencing GhTUBB1 expression in R15 and found that decreased expression resulted in a dwarf phenotype. Interestingly, we found that repressing AmCBF1 expression in L28 and L30 partly recovered the expression of GhTUBB1. Thus, AmCBF1 expression presented a negative relationship with GhTUBB1 expression in L28 and L30. Moreover, yeast one-hybrid and dual-luciferase assays suggest that AmCBF1 negatively regulates GhTUBB1 expression by directly binding to C-repeat/dehydration-responsive (CRT/DRE) elements in the GhTUBB1 promoter, potentially explaining the dwarf phenotypes of L28 and L30. This study elucidates the regulation of GhTUBB1 expression by AmCBF1 and suggests that GhTUBB1 may be a new target gene for breeding dwarf and compact cultivars.
Human Non-Small Cell Lung Cancer-Chicken Embryo Chorioallantoic Membrane Tumor Models for Experimental Cancer Treatments
Authors: Li Jing , Brachtlova Tereza , van der Meulen-Muileman Ida H. , Kleerebezem Stijn , Liu Chang , Li Peiyu , van Beusechem Victor W.
Published: October 2023

To promote the preclinical development of new treatments for non-small cell lung cancer (NSCLC), we established NSCLC xenograft tumor assays on the chorioallantoic membrane (CAM) of chicken embryos. Five NSCLC cell lines were compared for tumor take rate, tumor growth, and embryo survival. Two of these, A549 and H460 CAM tumors, were histologically characterized and tested for susceptibility to systemic chemotherapy and gene delivery using viral vectors. All cell lines were efficiently engrafted with minimal effect on embryo survival. The A549 cells formed slowly growing tumors, with a relatively uniform distribution of cancer cells and stroma cells, while the H460 cells formed large tumors containing mostly proliferating cancer cells in a bed of vascularized connective tissue. Tumor growth was inhibited via systemic treatment with Pemetrexed and Cisplatin, a chemotherapy combination that is often used to treat patients with advanced NSCLC. Lentiviral and adenoviral vectors expressing firefly luciferase transduced NSCLC tumors in vivo. The adenovirus vector yielded more than 100-fold higher luminescence intensities after a single administration than could be achieved with multiple lentiviral vector deliveries. The adenovirus vector also transduced CAM tissue and organs of developing embryos. Adenovirus delivery to tumors was 100–10,000-fold more efficient than to embryo organs. In conclusion, established human NSCLC-CAM tumor models provide convenient in vivo assays to rapidly evaluate new cancer therapies, particularly cancer gene therapies.
Metabolic and Transcriptomic Approaches of Chitosan and Water Stress on Polyphenolic and Terpenoid Components and Gene Expression in Salvia abrotanoides (Karl.) and S. yangii
Authors: Khodadadi Farzaneh , Ahmadi Farajollah Shahriai , Talebi Majid , Matkowski Adam , Szumny Antoni , Afshari Mahvash , Rahimmalek Mehdi
Published: October 2023

In this research, a HPLC analysis, along with transcriptomics tools, was applied to evaluate chitosan and water stress for the prediction of phenolic flavonoids patterns and terpenoid components accumulation in Salvia abrotanoides Karel and S. yangii. The results indicated that the tanshinone contents under drought stress conditions increased 4.2-fold with increasing drought stress intensity in both species. The rosmarinic acid content in the leaves varied from 0.038 to 11.43 mg/g DW. In addition, the flavonoid content was increased (1.8 and 1.4-fold) under mild water deficit conditions with a moderate concentration of chitosan (100 mg L−1). The application of foliar chitosan at 100 and 200 mg L−1 under well-watered and mild stress conditions led to increases in hydroxyl cryptotanshinone (OH-CT) and cryptotanshinone (CT) contents as the major terpenoid components in both species. The expressions of the studied genes (DXS2, HMGR, KSL, 4CL, and TAT) were also noticeably induced by water deficit and variably modulated by the treatment with chitosan. According to our findings, both the drought stress and the application of foliar chitosan altered the expression levels of certain genes. Specifically, we observed changes in the expression levels of DXS and HMGR, which are upstream genes in the MEP and MVA pathways, respectively. Additionally, the expression level of KSL, a downstream gene involved in diterpenoid synthesis, was also affected. Finally, the present investigation confirmed that chitosan treatments and water stress were affected in both the methylerythritol phosphate pathway (MEP) and mevalonate (MVA) pathways, but their commitment to the production of other isoprenoids has to be considered and discussed.
Effects of Prokineticins on Cerebral Cell Function and Blood–Brain Barrier Permeability
Authors: Younes Hadi , Kyritsi Ioanna , Mahrougui Zineb , Benharouga Mohamed , Alfaidy Nadia , Marquette Christel
Published: October 2023

Prokineticins are a family of small proteins with diverse roles in various tissues, including the brain. However, their specific effects on different cerebral cell types and blood–brain barrier (BBB) function remain unclear. The aim of this study was to investigate the effects of PROK1 and PROK2 on murine cerebral cell lines, bEnd.3, C8.D30, and N2a, corresponding to microvascular endothelial cells, astrocytes and neurons, respectively, and on an established BBB co-culture model. Western blot analysis showed that prokineticin receptors (PROKR1 and PROKR2) were differentially expressed in the considered cell lines. The effect of PROK1 and PROK2 on cell proliferation and migration were assessed using time-lapse microscopy. PROK1 decreased neural cells’ proliferation, while it had no effect on the proliferation of endothelial cells and astrocytes. In contrast, PROK2 reduced the proliferation of all cell lines tested. Both PROK1 and PROK2 increased the migration of all cell lines. Blocking PROKRs with the PROKR1 antagonist (PC7) and the PROKR2 antagonist (PKR-A) inhibited astrocyte PROK2-mediated migration. Using the insert co-culture model of BBB, we demonstrated that PROKs increased BBB permeability, which could be prevented by PROKRs’ antagonists.
Adipose Tissue-Derived Mesenchymal Stem Cells Extend the Lifespan and Enhance Liver Function in Hepatocyte Organoids
Authors: Ock Sun A , Kim Seo-Yeon , Ju Won Seok , Kim Young-Im , Wi Ha-Yeon , Lee Poongyeon
Published: October 2023

In this study, we generated hepatocyte organoids (HOs) using frozen-thawed primary hepatocytes (PHs) within a three-dimensional (3D) Matrigel dome culture in a porcine model. Previously studied hepatocyte organoid analogs, spheroids, or hepatocyte aggregates created using PHs in 3D culture systems have limitations in their in vitro lifespans. By co-culturing adipose tissue-derived mesenchymal stem cells (A-MSCs) with HOs within a 3D Matrigel dome culture, we achieved a 3.5-fold increase in the in vitro lifespan and enhanced liver function compared to a conventional two-dimensional (2D) monolayer culture, i.e., more than twice that of the HO group cultured alone, reaching up to 126 d. Although PHs were used to generate HOs, we identified markers associated with cholangiocyte organoids such as cytokeratin 19 and epithelial cellular adhesion molecule (EPCAM). Co-culturing A-MSCs with HOs increased the secretion of albumin and urea and glucose consumption compared to HOs cultured alone. After more than 100 d, we observed the upregulation of tumor protein P53 (TP53)-P21 and downregulation of EPCAM, albumin (ALB), and cytochrome P450 family 3 subfamily A member 29 (CYP3A29). Therefore, HOs with function and longevity improved through co-culturing with A-MSCs can be used to create large-scale human hepatotoxicity testing models and precise livestock nutrition assessment tools.
The Key Role of Astrocytes in Amyotrophic Lateral Sclerosis and Their Commitment to Glutamate Excitotoxicity
Authors: Provenzano Francesca , Torazza Carola , Bonifacino Tiziana , Bonanno Giambattista , Milanese Marco
Published: October 2023

In the last two decades, there has been increasing evidence supporting non-neuronal cells as active contributors to neurodegenerative disorders. Among glial cells, astrocytes play a pivotal role in driving amyotrophic lateral sclerosis (ALS) progression, leading the scientific community to focus on the “astrocytic signature” in ALS. Here, we summarized the main pathological mechanisms characterizing astrocyte contribution to MN damage and ALS progression, such as neuroinflammation, mitochondrial dysfunction, oxidative stress, energy metabolism impairment, miRNAs and extracellular vesicles contribution, autophagy dysfunction, protein misfolding, and altered neurotrophic factor release. Since glutamate excitotoxicity is one of the most relevant ALS features, we focused on the specific contribution of ALS astrocytes in this aspect, highlighting the known or potential molecular mechanisms by which astrocytes participate in increasing the extracellular glutamate level in ALS and, conversely, undergo the toxic effect of the excessive glutamate. In this scenario, astrocytes can behave as “producers” and “targets” of the high extracellular glutamate levels, going through changes that can affect themselves and, in turn, the neuronal and non-neuronal surrounding cells, thus actively impacting the ALS course. Moreover, this review aims to point out knowledge gaps that deserve further investigation.
Effects of C-Peptide on Dexamethasone-Induced In Vitro and In Vivo Models as a Potential Therapeutic Agent for Muscle Atrophy
Authors: Kim Jinjoo , Yang Youngmo , Choi Eunwon , Lee Sumin , Choi Jiyoung
Published: October 2023

This study aimed to investigate the effects of C-peptide on C2C12 myotubes and a mouse model. Both in vitro and in vivo experiments were conducted to elucidate the role of C-peptide in muscle atrophy. Various concentrations (0, 0.01, 0.1, 1, 10, and 100 nM) of C-peptide were used on the differentiated C2C12 myotubes with or without dexamethasone (DEX). C57BL/6J mice were administered with C-peptide and DEX for 8 days, followed by C-peptide treatment for 12 days. Compared to the DEX group, C-peptide increased the fusion and differentiation indices and suppressed atrophic factor expression in C2C12 myotubes. However, 100 nM C-peptide decreased the fusion and differentiation indices and increased atrophic factor expression regardless of DEX treatment. In C57BL/6J mice, DEX + C-peptide co-treatment significantly attenuated the body and muscle weight loss and improved the grip strength and cross-sectional area of the gastrocnemius (Gas) and quadriceps (Quad) muscles. C-peptide downregulated the mRNA and protein levels of muscle degradation-related markers, particularly Atrogin-1, in Gas and Quad muscles. This study underscores the potential of C-peptides in mitigating muscle weight reduction and preserving muscle function during muscle atrophy via molecular regulation. In addition, the work presents basic data for future studies on the effect of C-peptide on diabetic muscular dystrophy.
Regulatory Elements Outside Established Pou5f1 Gene Boundaries Are Required for Multilineage Differentiation of Embryonic Stem Cells
Authors: Ermakova Veronika V. , Fokin Nikita P. , Aksenov Nikolay D. , Bakhmet Evgeny I. , Aleksandrova Ekaterina V. , Kuzmin Andrey A. , Tomilin Alexey N.
Published: October 2023

The transcription factor Oct4 can rightfully be considered a pivotal element in maintaining pluripotency. In addition, its ability to function as a pioneer factor enables the reprogramming of somatic cells back into a pluripotent state. To better understand the regulation of the Oct4-encoding gene (Pou5f1), the main genetic elements that regulate its expression in different states of pluripotency ought to be identified. While some elements have been well characterized for their ability to drive Pou5f1 expression, others have yet to be determined. In this work, we show that translocation of the Pou5f1 gene fragment purported to span all essential cis-elements, including the well-known distal and proximal enhancers (DE and PE), into the Rosa26 locus impairs the self-renewal of mouse embryonic stem cells (ESCs) in the naïve pluripotency state, as well as their further advancement through the formative and primed pluripotency states, inducing overall differentiation failure. These results suggest that regulatory elements located outside the previously determined Pou5f1 boundaries are critical for the proper spatiotemporal regulation of this gene during development, indicating the need for their better characterization.
Mobility Control Centre and Artificial Intelligence for Sustainable Urban Districts
Authors: Cirianni Francis Marco Maria , Comi Antonio , Quattrone Agata
Published: October 2023

The application of artificial intelligence (AI) to dynamic mobility management can support the achievement of efficiency and sustainability goals. AI can help to model alternative mobility system scenarios in real time (by processing big data from heterogeneous sources in a very short time) and to identify network and service configurations by comparing phenomena in similar contexts, as well as support the implementation of measures for managing demand that achieve sustainable goals. In this paper, an in-depth analysis of scenarios, with an IT (Information Technology) framework based on emerging technologies and AI to support sustainable and cooperative digital mobility, is provided. Therefore, the definition of the functional architecture of an AI-based mobility control centre is defined, and the process that has been implemented in a medium-large city is presented.
Multi-Parametric Delineation Approach for Homogeneous Sectioning of Asphalt Pavements
Authors: Peraka Naga Siva Pavani , Biligiri Krishna Prapoorna , Kalidindi Satyanarayana N.
Published: October 2023

The demand for preserving existing roadway infrastructure has been increasing to regulate expensive reconstruction activities. The maintenance of homogeneous road sections is one of the approaches to economize the overall management of pavement systems. The existing homogeneous delineation methods consider one or two parameters for segmenting the pavements based on similar characteristics, which are found to be a repetitive process. Also, there is a need to consider multiple parameters that represent the functional, structural, and traffic characteristics in segmentation process. Therefore, the objective of this study was to develop a multi-parameter-based delineation approach (MPDA) to segment the pavements into subsections with similar features considering functional, structural, and traffic characteristics. Deflection bowl parameters, unified pavement health index (functional performance metric), surface layer modulus, and traffic reported in terms of AADT were employed for developing a multi-parametric delineation index (MPDI). A total of 1781 datapoints covering 26 road sections in the State of Andhra Pradesh, India, were used. The C-charts method-based segmentation for MPDI was applied to obtain the homogeneous sections. The devised approach was found to be efficient in segmenting the pavements as well as robust in selecting suitable maintenance strategies for each group of the homogeneous sections. Further, the segmentation processes were automated for easier implementation by the agencies.
Large Male Caterpillars Are the Primary Builders: Exploring Tent Construction and Foraging Behaviour in Gregarious Pine Processionary Caterpillar
Authors: Uemura Mizuki , Zalucki Myron P. , Battisti Andrea
Published: October 2023

As a social organism, living in a communal structure is one of the most important physical barriers against environmental elements and natural enemies. Thaumetopoea pityocampa (Notodontidae, Thaumetopoeinae) caterpillars are conifer pests that spend most of their larval stage in winter. Although T. pityocampa holds economic and medical significance, the tent construction and foraging behaviour are poorly understood. We observed the tent construction behaviour in autumn (October and November) when third- and fourth-instar T. pityocampa caterpillars build the ‘winter tent’ that can withstand winter conditions. Just before sunset, with no rain and temperatures over 12 °C, tent construction was undertaken by early active individuals, primarily larger male caterpillars. Early active caterpillars emerge from the tent first and spin silk on the tent for expansion and strength. Once temperatures dropped below 12 °C and twilight had passed, the early active caterpillars went out to forage and were later joined by the late active caterpillars, which were predominantly smaller females that had remained inside the tent. Foraging behaviour was continuously monitored for the first to fourth larval instars in the field. Foraging was more frequent in younger instars when environmental temperatures were warmer and became continuous and prolonged in later instar caterpillars as temperatures dropped. The final tent structure built by later instar caterpillars had the thickest layer of silk on the southern side of the tent compared to other orientations to receive maximum solar radiation during the winter. Our study provided additional insights into the collective nest building, foraging and social behaviours observed in Lepidoptera, as well as the roles of individuals within non-eusocial insect colonies.
Effect of Molybdenum Coatings on the Accelerating Cavity Quality Factor
Authors: Vidal García Pablo , Sarti Stefano , Carillo Martina , Giuliano Lucia , Marcelli Augusto , Spataro Bruno , Alimenti Andrea , Torokhtii Kostiantyn , Silva Enrico , Pompeo Nicola
Published: October 2023

In this work, a detailed parametric study assessing the impact of low-conductivity coatings on the radio-frequency accelerating cavity quality factor and resonance frequency shift is presented. In particular, this study is aimed at proving the feasibility of molybdenum oxides deposited on copper to reduce the dark current in high-gradient applications due to its intrinsically high work function. In order to compute the effective surface impedance of the resulting layered structure, a transmission line-based approach is adopted. The present analysis demonstrates the potential effectiveness of molybdenum thin-films, which only slightly affects the accelerating cavity quality factor, with very low sensitivity to thickness and resistivity inhomogeneities.
Could We Expect Postoperative Cup Anteversion after Total Hip Arthroplasty Using Postoperative Plain Anteroposterior and Lateral Radiograph? A Three-Dimensional Experimental Operation Study
Authors: Lee Tae Sung , Kwon Hyuck Min , Park Jun Young , Kim Dong Ki , Kang Kyoung Tak , Park Kwan Kyu
Published: October 2023

Background: A postoperative radiograph in total hip arthroplasty (THA) is usually obtained to evaluate the inclination and anteversion of the acetabular components. However, there is no gold-standard method for calculating the exact inclination and anteversion of the acetabular components on post-THA radiographs. We aimed to measure the actual anteversion of the acetabular component on postoperative radiographs by obtaining correlation data between the virtual and actual acetabular component positioning using virtual three-dimensional (3D) surgery. Methods: A total of 64 hip scans of 32 patients who underwent lower-extremity computed tomography (CT) were retrospectively reviewed. We reconstructed 3D models of the 64 hips using customized computer software (Mimics). Furthermore, to identify the safe zone of acetabular component position in THA, we performed virtual 3D surgery simulations for five anteversion (−10°, 0°, 10°, 20°, and 30°) and five inclination (20°, 30°, 40°, 50°, and 60°) types. We analyzed the acetabular anatomy using 3D models to measure the radiographic, anatomical, and operative anteversion (RA, AA, OA) and inclination (RI, AI, OI) angles. Additionally, we used the Woo–Morrey (WM) method to calculate the anteversion angle in the reconstructed cross-table lateral (CL) radiographs and determined the correlation between these measurements. Results: The safe zone of the acetabular component was visualized on post-THA CL radiographs using the WM method of anteversion measurement based on the different anteversions and inclinations of the acetabular component. The AA, RA, OA, OI, and WM differed significantly between males and females (p value < 0.05). As the anatomical inclination or anteversion increased, the WM anteversion measurements also increased. The radiographic anteversion measurement best matched the WM method of measurement, followed by anatomical and operative methods. Conclusions: The actual anteversion of the acetabular component after THA can be measured on CL radiographs with the WM method using a 3D virtual program, with good reproducibility.
The Influence of History of Severe Periodontitis on Estimated Long-Term Marginal Bone Loss around Implants Restored with Fixed Segmented Full-Arch Rehabilitation
Authors: Galindo-Moreno Pablo , Catena Andres , Lopez-Chaichio Lucia , Borges Tiago , O’Valle Francisco , Torrecillas-Martínez Laura , Padial-Molina Miguel
Published: October 2023

The aim of this study was to analyze the long-term marginal bone level (MBL) of implants supporting fixed full-arch restoration in patients who had previously lost their dentition due to severe periodontitis. This retrospective study included 35 patients in whom 342 implants with internal tapered conical connections were placed. MBL was analyzed radiographically over time and a long-term estimation of MBL was calculated. A mixed linear model with abutment height, graft, diameter and location (maxilla/mandible) as factors and gender, age, implant length and prosthetic variables as covariates was used to evaluate the influence on MBL. MBL in these patients showed an estimator of predictions at 4108 days after loading of −0.307 mm, SE = 0.042. Only 0.15% of implants were radiographically affected with MBL of 3 mm or more. The mixed linear model results showed a main effect of the type of opposing dentition, gender, implant diameter, and abutment height. Particularly, an abutment height of 1 mm had associated larger MBL than the remaining heights. Thus, it can be concluded that dental implants restored with fixed segmented full-arch rehabilitation in patients with a history of severe periodontal disease do not suffer important marginal bone loss if some specific factors are considered, mainly the use of long transmucosal abutments (≥2 mm).
Therapeutic and Preventive Interventions in Adolescents with Borderline Personality Disorder: Recent Findings, Current Challenges, and Future Directions
Authors: Bourvis Nadège , Cohen David , Benarous Xavier
Published: October 2023

Background: Borderline personality disorder (BPD) has long suffered from overshadowing in adolescents and hopelessness from the psychiatrists themselves. Comprehensive guidelines for this age group are lacking. Aims: This narrative review aims to describe current recommendations for BPD and recent empirical evidence on effective treatments (both pharmacological and non-pharmacological) and preventive approaches. Innovative approaches, based on recent and original research on BPD adolescents, are also discussed. Results: Very low-certainty evidence has supported that medication has a positive effect on core BPD symptoms in adolescents. Medication prescribed for suicidal crises or associated disorders should be included in a global therapeutic plan, including efficacy reassessment, treatment duration, and a security plan. The overall benefit of structured psychotherapy for adolescents with BPD (cognitive behavioral therapy, mentalization-based therapy, dialectic behavioral therapy, and group therapy) is more important for self-harm behaviors than other BPD symptoms. Their specific efficacy, although difficult to distinguish from the overall non-specific effect of integrative care. Conclusions: structured care of young BPD individuals should be based on the following principles: (1) setting the frame of care, including recognition of the diagnosis, and sharing information with patients and families about symptoms, prognosis, and putative psychological mechanisms involved; and (2) promoting comprehensive approaches, including both specific and non specific therapy, ecological interventions, community care, and preventive interventions in at-risk groups.
Biomass Derived High Porous Carbon via CO2 Activation for Supercapacitor Electrodes
Authors: Taurbekov Azamat , Abdisattar Alisher , Atamanov Meiram , Yeleuov Mukhtar , Daulbayev Chingis , Askaruly Kydyr , Kaidar Bayan , Mansurov Zulkhair , Castro-Gutierrez Jimena , Celzard Alain , Fierro Vanessa , Atamanova Tolganay
Published: October 2023

In this study, we systematically study the efficient production method and electrochemical characteristics of activated carbons (AC) derived from rice husk (RH) and walnut shell (WS). In particular, the effectiveness of physical activation using carbon dioxide (CO2) was investigated and compared with the more common chemical activation method using potassium hydroxide (KOH). The results show that the KOH–activated samples have remarkable specific capacities, reaching 157.8 F g−1 for RH and 152 F g−1 for WS at 1 A g−1. However, the rate capability of AC obtained via KOH decreases significantly as the scanning rate increases, retaining only 51.5% and 68% of their original capacities for RH–KOH and WS–KOH, respectively, at 20 A g–1. In contrast, CO2–activated samples show a superior rate performance with a capacity retention of 75.6% for WS and 80% for RH at the same current density. In addition, electrochemical impedance spectroscopy (EIS) analysis shows that AC obtained via CO2 has a lower charge transfer resistance compared to its KOH counterparts. CO2–activated RH and WS electrodes show Rct values of 0.1 Ω and 0.24 Ω, respectively, indicating improved ion transport kinetics and surface area utilization. These results highlight the importance of activation techniques in tailoring the electrochemical behavior of biomass–derived carbon. This study not only expands the understanding of the interaction between activation, morphology, and performance but also indicates the potential of CO2 activation as an environmentally friendly and efficient alternative. As the field of sustainable energy storage advances, this work provides valuable guidance for the development of high–performance supercapacitor electrodes with less environmental impact.
An In Vitro Study regarding the Wear of Composite Materials Following the Use of Dental Bleaching Protocols
Authors: Popescu Alexandru Dan , Ţuculină Mihaela Jana , Gheorghiță Lelia Mihaela , Osman Andrei , Nicolicescu Claudiu , Bugălă Smaranda Adelina , Ionescu Mihaela , Abdul-Razzak Jaqueline , Diaconu Oana Andreea , Dimitriu Bogdan
Published: October 2023

Composite materials used in dental restorations are considered resistant, long-lasting and aesthetic. As the wear of restorations is an important element in long-term use, the aim of this study was to evaluate the surface condition of nanohybrid and microfilled composite resins, after being subjected to the erosive action of dental bleaching protocols. This paper reflects a comparative study between one nanofilled composite and three microfilled composites used in restorations. For each composite, three sets of samples (under the form of composite discs) were created: a control group, an “office bleach” group with discs bleached with 40% hydrogen peroxide gel, and a “home bleach” group with discs bleached with 16% carbamide peroxide gel. Wear was numerically determined as the trace and the coefficients of friction obtained using a tribometer, the ball-on-disk test method, and two balls: alumina and sapphire. For all composite groups, there were statistically significant differences between the wear corresponding to the control and bleaching groups, for both testing balls. Regarding the composite type, the largest traces were recorded for GC Gradia direct anterior, for all groups, using the alumina ball. In contrast, for the sapphire ball, 3M ESPE Filtek Z550 was characterized by the largest traces. With respect to the friction coefficients, the “office bleach” group recorded the largest values, no matter the composite or the ball type used. The 3M ESPE Valux Plus composite recorded the largest friction coefficients for the alumina ball, and 3M ESPE Filtek Z550 for the sapphire ball. Overall, the “office bleach” group was characterized by higher composite wear, compared to the “home bleach” protocol or control group. Nanofilled composite resins showed superior wear resistance to microfilled resins after undergoing a bleaching protocol.
A Study of PI Controller Tuning Methods Using the Internal Model Control Guide for a Ship Central Cooling System as a Multi-Input, Single-Output System
Authors: Jeon Tae-Youl , Jung Byung-Gun
Published: October 2023

Since the variable-speed seawater pump and the three-way valve of a ship’s central cooling system are forms of feedback from the same output signal, these controllers may cause interference when they are not coordinated. Therefore, studying an efficient control tuning method for a central cooling water system is necessary. In this study, a central cooling water system is modeled using Matlab Simulink by utilizing an actual operation dataset, and unknown parameters are estimated for fine-tuning. The simulation model is then verified using the second operating dataset. Additionally, transfer functions are developed for the freshwater output temperature against the three-way valve openness input and the electrical power frequency input to the seawater pump motor, supposing that the two systems are independent. Then, the two PI controllers are tuned using internal model control (IMC) filters. Moreover, the modified internal model control tuning method is suggested, using the character of the central cooling system, which has a large time constant for the heat exchanger system with the seawater pump and a small time constant for the three-way valve system. This method simplifies the tuning of the two combined PI controllers, enhancing the seawater pump’s overall efficiency and the three-way valve’s operation. This study presents the proposed tuning methods based on the IMC filter and modified IMC guide, which confirmed the simple determination of the gain values of the PI controller with the efficient control of the rotational speed of the seawater pump and the three-way valve with reasonable control of the freshwater outlet temperature.
Impact of Ultrasound–Assisted Method on Success Rate of Spinal Anesthesia Performed by Novice Trainees: A Retrospective Comparative Study
Authors: Coviello Antonio , Iacovazzo Carmine , Piccione Ilaria , Posillipo Concetta , Barone Maria Silvia , Ianniello Marilena , de Siena Andrea Uriel , Cirillo Dario , Vargas Maria
Published: October 2023

In current practice, single-shot spinal anesthesia has traditionally been performed using the conventional surface-anatomic-Landmark-Guided technique. This “blind” technique has significant critical issues such as a high risk of complications due to the numerous attempts at spinal needle placement and the negative impact on the learning curve of the trainees. Ultrasound-Assisted spinal anesthesia could reduce these critical issues and allow trainees to perform the procedure more easily and with fewer complications for the patient. We performed a before-and-after monocentric retrospective comparative study at the University of Naples “Federico II” (Naples, Italy). Inclusion criteria were as follows: patients aged 18 years or older; ASA physical status between I and IV; and elective orthopedic surgery under single-shot spinal anesthesia performed by supervised trainees between January 2022 and December 2022. In the selected cohort, 88 patients were included in group A (Landmark-Guided spinal anesthesia) and 91 in group B (Ultrasound-Assisted spinal anesthesia). The number of attempts by trainees (p-value < 0.005), procedure performing time (<0.001), and patient discomfort (<0.001) were significantly lower in group B than in group A. Ultrasound-Assisted single-shot spinal anesthesia performed by novice trainees reduces the number of attempts, complication rate, periprocedural pain, and patient discomfort.
Does Fintech-Driven Inclusive Finance Induce Bank Profitability? Empirical Evidence from Developing Countries
Authors: Zheng Changjun , Rahman Md Ataur , Hossain Shahadat , Moudud-Ul-Huq Syed
Published: October 2023

This study explores the effect of fintech-driven inclusive finance on the profitability of banks using an unbalanced panel dataset from 660 banks across 40 developing countries between 2011 and 2021. We start with a fixed-effect estimate and subsequently validate our main findings using two-stage least squares (2SLS-IV), two-step system generalized method of moments (GMM), and generalized least squares (GLS) methodologies. Our analysis centers on three key profitability metrics: ROA, ROE, and NIM. Our findings suggest that fintech-backed inclusive finance boosts ROA by 9.10%, ROE by 18.87%, and NIM by 7.98%, highlighting the growing importance of mobile, internet, and agent banking in these nations. We also note that large banks benefit more from inclusive finance than small ones. Additionally, conventional banks see a more marked improvement in profitability than Islamic and savings banks. The relationship between inclusive finance and bank profitability is stronger in countries with higher GDP growth and those actively advancing financial inclusion through fintech, compared to countries with slower GDP growth and less emphasis on financial inclusion. When examining the interaction effects, the COVID-19 pandemic has further emphasized the positive connection between fintech and bank profitability. This suggests that fintech-driven inclusive finance can play a role in enhancing bank profitability, even in challenging times like the COVID-19 period. The transition towards fintech, however, mandates substantial investments, enhanced financial literacy, and heightened customer security, presenting persistent challenges for governments, policymakers, regulators, and financial institutions.