bluuebunny/crossref_metadata_2025 huggingface dataset:
Sustainable intensification of small-scale mariculture systems: Farm-level insights from the coastal regions of India
Authors: Parappurathu Shinoj , Menon Muktha , Jeeva Charles , Belevendran Johnson , Anirudhan Anuraj , Lekshmi P. S. Swathi , Ramachandran C. , Padua Shelton , Aswathy Natarajan , Ghosh Shubhadeep , Damodaran Divu , Megarajan Sekar , Rajamanickam Geetha , Vinuja S. V. , Ignatius Boby , Raghavan Suresh Vettath , Narayanakumar Ramani , Gopalakrishnan Achamveetil , Chand Prem
Published: March 2023

This study undertakes a comprehensive assessment of selected mariculture enterprises in the coastal regions of India, centered on long-term sustainability as the key focus. This is juxtaposed against India's ambitious blue economy targets and policy thrust that pin on the expansion of mariculture as a promising avenue for enhancing marine fish production. Farm-level, region-specific, techno-economic, and socio-cultural factors associated with, and conditional on, sustainable intensification of mariculture-based production systems are examined in detail. The Principles-Criteria-Indicators (PCI) approach is used to establish the linkage between identified farm-level indicators and various dimensions of sustainability. While the selected enterprises were assessed to be technically and economically viable in general, glaring gaps were evident on key indicators of sustainability such as the legitimacy of access over water bodies, use of quality seed and feed, institutional credit access, market access, and fair marketing practices, optimal stocking density, mechanization, use of renewable energy, adoption of environmental-friendly culture practices, farm surveillance, crew safety, and social protection. This indicates the need for taking proactive measures to ensure the long-term sustainability of mariculture, particularly in the initial stages of establishment when such interventions are easy to adopt. Based on the insights obtained from the analysis, a broad set of strategies, policy options, and institutional interventions critical to scaling-up coastal mariculture enterprises along the east and west coasts of India are presented.
Harnessing the efficacy of multifunctional rhizobacterial consortia for promoting the growth of Anethum graveolens L.
Authors: Joshi Nishra , Saraf Meenu , Jha Chaitanya Kumar , Sudha A. , Alharbi Sulaiman Ali , Alfarraj Saleh , Datta Rahul
Published: March 2023

Co-cultures of bacteria are more metabolically flexible and more tolerant to changes in the environment than single cultures. In order to test for plant growth promotion in a medicinal herb Anethum graveolens L, potent phosphate-solubilizing rhizobacteria were selected, characterized and assessed for their compatibility with each other. Molecular identification of isolates was made by 16s rRNA sequence, and they were identified as Pseudomonas aeruginosaNJC4 (OP289324), Serratia marcescens NJC21 (OP289323) and Bacillus spp. Dual species consortia, namely, Bacillus spp. + Serratia marcescens NJC21 (T1), and Pseudomonas aeruginosa NJC4 + Serratia marcescens NJC21 (T2), were tested for their ability to produce multiple plant beneficial activities such as phosphate solubilization, and ammonia and indole acetic acid production. The best isolate and consortium were evaluated for plant growth promotion activity. A plant treated with consortia T-2 seemed most effective in seed emergence at 84.66%, which was four times superior to the control. Growth and yield characters, along with all different rhizobacterial treatments, were examined by principal component analysis (PCA), where PC1 can explain 51.37% of the total variance and PC2 can explain 26.75%. PC1 was associated with wet biomass, chlorophyll b, and total chlorophyll content, which reflect the strong influence of consortia T-1. At the same time, PC2 was found to be related to dry biomass and chlorophyll a content. This study lends credence to the theory that microbial consortiums consisting of more than one efficient strains may be more effective than single cultures in boosting the increase of agricultural output in a sustainable way.
The effect of fertilization with microfiltered liquid digestate on the quality parameters of Citrus fruits
Authors: Castellano Angela , Selvaggi Roberta , Mantovi Paolo , Spina Daniela , Hamam Manal , Pappalardo Gioacchino
Published: March 2023

Nowadays, the adoption of sustainable agricultural practices, including the reduction of synthetic fertilizers, has become a challenge for the agriculture sector. In this experimental work, the effect of the liquid fraction of digestate (by-product of the anaerobic digestion process) as a fertilizer was evaluated. The aim of the research was to verify to which extent digestate can affect growth and quality parameters of orange fruits, comparing the results to those obtained for fruits grown on soil treated with conventional mineral fertilizers. To assess the effectiveness of the treatments, different qualitative and quantitative parameters of Citrus fruits were measured. In particular, the results showed slight differences between the two treatments, suggesting that digestate may be used for the production of high-quality fruits. Moreover, in some orchards, the Citrus fruits of the plants treated with digestate showed a higher concentration of health-promoting compounds, such as vitamin C, flavonoids, phenolic content, when compared to the control group. Thus, digestate can be considered an optimal source of plant nutrients and can be used as a crop growth promoter, since it represents an effective strategy for reducing the mineral fertilizers input.
The relationship between Ki-67 expression and imaging signs and pathological features in GISTs
Authors: Xiao Lin , Zhang Yiding , Wang Yajie , Liu Lede , Pan Yisheng
Published: March 2023

Introduction

To investigate the correlations between the Ki-67 index and plain-scan computerized tomography (CT) signs and pathological features of gastrointestinal stromal tumor (GIST) tissue.

Materials and methods

Data from 186 patients with GIST diagnosed by pathology and immunohistochemistry (IHC) in Peking University First Hospital from May 2016 to May 2022 were analyzed. The patients were divided into two groups: Ki-67 ≤5% and >5%. Correlation analysis, univariate and multivariate Logistic regression analysis were used to explore the correlations between CT signs, pathological features, and Ki-67 expression.

Results

Univariate indicators correlated with the Ki-67 index were mitotic count, pathological grade, tumor hemorrhage, tumor necrosis, tumor size, and tumor density. Multivariate Logistic regression indicated that the mitotic count [odds ratio (OR) 10.222, 95% confidence interval (CI) 4.312–31.039], pathological grade (OR 2.139, 95% CI 1.397–3.350), and tumor size (OR 1.096, 95% CI 1.020–1.190) were independently associated with the Ki-67 expression level. The concordance indexes (C-index) for the pathological features and CT signs models were 0.876 (95% CI 0.822–0.929) and 0.697 (95% CI 0.620–0.774), respectively, with positive predictive values of 93.62% and 58.11% and negative predictive values of 81.29% and 75.89%, respectively. After internal verification by the Bootstrap method, the fitting degree of the pathological features model was found to be better than that of the CT signs model.

Conclusion

Mitotic count, pathological risk grading, and tumor size are independent risk factors correlating with high Ki-67 index. These results indicate that the Ki-67 index reflects tumor malignancy and can predict recurrence and metastasis of GIST.
Distinct mechanisms underlie electrical coupling resonance and its interaction with membrane potential resonance
Authors: Li Xinping , Itani Omar , Bucher Dirk M. , Rotstein Horacio G. , Nadim Farzan
Published: March 2023

Neurons in oscillatory networks often exhibit membrane potential resonance, a peak impedance at a non-zero input frequency. In electrically coupled oscillatory networks, the coupling coefficient (the ratio of post- and prejunctional voltage responses) could also show resonance. Such coupling resonance may emerge from the interaction between the coupling current and resonance properties of the coupled neurons, but this relationship has not been clearly described. Additionally, it is unknown if the gap-junction mediated electrical coupling conductance may have frequency dependence. We examined these questions by recording a pair of electrically coupled neurons in the oscillatory pyloric network of the crab Cancer borealis. We performed dual current- and voltage-clamp recordings and quantified the frequency preference of the coupled neurons, the coupling coefficient, the electrical conductance, and the postjunctional neuronal response. We found that all components exhibit frequency selectivity, but with distinct preferred frequencies. Mathematical and computational analysis showed that membrane potential resonance of the postjunctional neuron was sufficient to give rise to resonance properties of the coupling coefficient, but not the coupling conductance. A distinct coupling conductance resonance frequency therefore emerges either from other circuit components or from the gating properties of the gap junctions. Finally, to explore the functional effect of the resonance of the coupling conductance, we examined its role in synchronizing neuronal the activities of electrically coupled bursting model neurons. Together, our findings elucidate factors that produce electrical coupling resonance and the function of this resonance in oscillatory networks.
How to facilitate the implementation of 3D models in China by applying good in vitro method practice for regulatory use
Authors: Liu Yanfeng , Cai Zhenzi , Li Nan , Alépée Nathalie
Published: March 2023

The Organization for Economic Co-operation and Development (OECD) Guidance Document No. 34 and No. 286 on Good In Vitro Method Practices (GIVIMPs) for the development and implementation of in vitro methods for regulatory use in human safety assessment have been endorsed. Considering that China is accelerating the development of alternative approaches in both research and acceptance, early application of these principles is beneficial to the implementation and acceptance of in vitro alternative methods in China. To promote the replacement of animal testing for regulatory use, L’Oréal initiated the EpiSkin™ skin irritation test (SIT) implementation program in China. More than 50 external scientists participated, and the method has been established in 34 organizations including authorities, industries, and testing service laboratories. Taking two collaborations with Guangdong CDC and Shanghai SGS for in vitro SIT as examples, we demonstrated a method implementation process in good alignment with the OECD principles. The current study illustrated the practical way in which both OECD Guidance documents assisted in the transfer and establishment of in vitro approaches and further promoted the future scientific recognition and acceptance of new OECD-accepted alternative testing methodologies in China.
Wykorzystywanie wybranych materiałów do terapii afazji w dydaktyce języka
polskiego jako obcego na niższych poziomach zaawansowania językowego (A1 i A2) .
Authors: Jędryka Beata Katarzyna
Published: March 2023

The author takes up issues concerned with teaching Polish as a foreign language with the use of speech therapy tools present in the treatment of patients suffering from aphasia. On the example of selected rehabilitation materials, she shows the extent to which they can be used in classes of Polish as a foreign language at levels A1 and A2. The enumerated tools can be used in PFL didactics to develop lexical, grammatical, and cultural competences.
Keywords: Polish as a foreign language – aphasia – didactics – language competence – speech therapy
Syntagmy skonwencjonalizowane zawierające
wyrazy głęboki i глибокий w języku polskim i ukraińskim
Authors: Bundza Iryna , Nikołajczuk Chrystyna
Published: March 2023

This article is devoted to the issues related to the semantic connectivity of the words głęboki and глибокий (deep) within their respective meanings. The study is based both on the information contained in dictionaries of both languages and on corpus data (the resources of the balanced versions of the NKJP and the ГРАК). All collocates of both adjectives were excerpted from the lexicographic and corpus material, and then typical – conventionalised – syntagmas with lexemes głęboki and глибокий were revealed. As a result of the in-depth analysis, conclusions were drawn also about the similarities within the semantic connectivity of the examined adjectives.
Opozycja swój – obcy w wizualno-werbalnych metaforach
publicystycznych
Authors: Maliszewski Bartłomiej
Published: March 2023

Based on covers of weekly magazines, it is easy to notice that the focal point of mass media is conflict situations. Their comments lead to the polarisation friend – foe, which is expressed by the visual and verbal presentation of the world of politics.
Despite the fact that the visual and verbal repertoire, which allows the positive evaluation of friends and negative evaluation of foes, is quite plentiful, clean patterns in portraying individuals are noticeable. Foes are aggressive, unpredictable, menacing, rapacious, dishonest and, at the same time, determined to build their positive image in order to attract new voters. Friends, in turn, are the victims of stalkers and their actions (the target of their attacks) and the long-awaited (true) saviours.
Magazine covers evidence social divisions and simultaneously consolidate and develop them, being an element of a relentless struggle to win audience and voters. This is because the marketing (attracting readers’ attention) and political (uniting the audiences against specified antagonists and around the figure of the depicted hero) aspects are of great importance here.
The Relationship between Leadership in BPM and Company Profitability
Authors: Gošnik Dušan , Meško Maja , Stubelj Igor
Published: March 2023

This paper studies the relationship between leadership as an activity of business process management (BPM) and company performance. Business process data about leadership and business processes in SMEs were collected via questionnaires on the population of 3007 SMEs in Slovenia. Aspects of leadership such as the involvement of employees, middle management, customers and suppliers were studied. The financial data of the SMEs of the sample were obtained from publicly available financial statements to assess relative residual income profitability ROEr and ROEa. Data analysis was performed using Bartlett’s, Kaiser–Maier–Olkin and nonparametric Mann–Whitney U tests. We confirmed a positive relationship between employee involvement in leadership as an activity of BPM and company profitability. It was proved that companies that involve employees and middle management in improving core business processes to a greater degree are more profitable. Intense involvement of employees in changes in core business processes results in higher company profitability, which has been detected by the higher risk-adjusted profitability measure ROEa. Companies that involve middle management to a greater degree by leading employees based on their interests are more profitable. The results are important for managers as decision-makers and other company stakeholders, especially those responsible for business process improvements. Theoretical and practical implications and further research possibilities are discussed.
Evaluation of Resistance to Stem Rust and Identification of Sr Genes in Russian Spring and Winter Wheat Cultivars in the Volga Region
Authors: Baranova Olga , Solyanikova Valeriya , Kyrova Elena , Kon’kova Elmira , Gaponov Sergey , Sergeev Valery , Shevchenko Sergey , Mal’chikov Pyotr , Dolzhenko Dmitrij , Bespalova Lyudmila , Ablova Irina , Tarhov Aleksandr , Vasilova Nuraniya , Askhadullin Damir , Askhadullin Danil , Sibikeev Sergey
Published: March 2023

The Volga region is one of the main grain-producing regions of Russia. Wheat stem rust caused by Puccinia graminis f. sp. tritici is among the most destructive fungal diseases of wheat. Recently, its harmfulness has increased in the Volga region. In this regard, an analysis of the resistance and diversity of the Sr genes in the Russian wheat cultivars is necessary. In this work, 126 wheat cultivars (including 23 durum wheat cultivars and 103 bread wheat cultivars) approved for use in the Volga region were evaluated for their resistance to two samples of P. graminis f. sp. tritici populations from different Volga region areas at the seedling stage. Specific DNA primers were used to identify resistance genes (Sr2, Sr24, Sr25, Sr26, Sr28, Sr31, Sr32, Sr36, Sr38, Sr39, and Sr57). Highly resistant cultivars (30 from 126) were identified. In bread wheat cultivars, the genes Sr31 (in 19 cultivars), Sr24 (in one cultivar), Sr25 (in 15 spring wheat cultivars), Sr28 (in six cultivars), Sr38 (in two cultivars), and Sr57 (in 15 cultivars) and their combinations—Sr31 + Sr25, Sr31 + Sr38, Sr31 + Sr28, Sr31 + Sr57, Sr31 + Sr28 + Sr57, and Sr31 + Sr24—were identified. The obtained results may be used to develop strategies for breeding rust-resistant cultivars.
Increasing the Durability of Tools for Forest Road Maintenance
Authors: Vargová Monika , Bołoz Łukasz , Ťavodová Miroslava , Hnilica Richard
Published: March 2023

To ensure the care of forests, it is necessary to make them sufficiently accessible by forest roads. The basic working tool are hammers, or round shanks of various shapes, composed of a body and a tip. They are subject to a strong abrasive environment, which often leads to damage up to the complete destruction of the functional part of the tool. For these reasons, it is necessary to deal with the possibilities for increasing their lifetime. One of the possibilities of increasing the service life of these tools is hardfacing by welding. The article deals with the abrasive resistance of the original material of the tool and the hardfacing materials. Based on the chemical analysis of the base material of the tool, we found that the tool is made of manganese steel 38Mn6. This material was used as a standard and was compared with the hardfacing materials Abradur 58, E DUR 600, UTP DUR 600 and OK 84.58. Electron microscopy was used to evaluate the microstructure. Next, the Rockwell hardness measurement was performed on the samples. The original tool material 38Mn6 reached the lowest hardness value, namely, 21 HRC. The highest value was reached by the hardfacing material E DUR 600, namely, 59 HRC. Subsequently, a test of resistance to abrasive wear was performed according to GOST 23.208-79. Based on this test, we can conclude that the highest value of resistance to abrasive wear was achieved by Abradur 58. Even though the hardness of this coating was slightly lower than the hardfacing material E DUR 600, specifically 56 HRC, we can state that this hardfacing material (Abradur 58) achieved the best results among the investigated materials.
Yield, Quality, and Water and Fertilizer Partial Productivity of Cucumber as Influenced by the Interaction of Water, Nitrogen, and Magnesium
Authors: Li Jinglai , Yang Xiaoqing , Zhang Mengchi , Li Dayong , Jiang Yu , Yao Wenhui , Zhang Zhi
Published: March 2023

The balanced management of water and nutrient factors is essential for the high-efficiency production of cucumber. The effect of magnesium coupling with water and nitrogen on cucumber was determined using a three-factor and three-level orthogonal rotation combination design experiment, and the yield, quality, and water and fertilizer efficiency of cucumber were studied. Yield was significantly influenced by the single factor of irrigation or nitrogen, with the largest value of 88,412.6 kg/ha under high levels of irrigation and nitrogen input. The increase in magnesium fertilizer has a significant promoting effect on reducing sugar and free amino acids, with results 19.0% and 9.8% higher than that of low magnesium application, respectively. The interaction of irrigation and magnesium alleviated the negative effects of nitrogen deficiency, thereby reducing the risk of nitrate accumulation and improving the partial productivity of nitrogen fertilizer. The interaction of nitrogen and magnesium significantly affected the yield of cucumber, and all the quality indicators except vitamin C and the partial productivity of water and fertilizer. Six indicators from three categories of yield, quality, and efficiency were used to establish the comprehensive evaluation system based on correlation analysis, and yield was assigned the highest combined weight of 0.4023 using game theory. Grey relational analysis model was adopted to evaluate the water and fertilizer treatments, and the optimal applied combination was irrigation of 653.7 m3/hm2, nitrogen fertilizer (CH4N2O) of 1141.9 kg/ha, and magnesium fertilizer (MgSO4.7H2O) of 422.1 kg/ha. This condition comprehensively promoted yield, quality, and efficiency, providing a scientific water and fertilizer management strategy for cucumber production in Northwest China.
Litter Decomposition Characteristics and Variety Differences in a Kiwifruit Orchard in Subtropical Climate Zone of China
Authors: Lu Yupeng , Gao Zhu , Mao Jipeng , Chen Lu , Zhang Xiaoli , Wang Xiaoling
Published: March 2023

The aim of this study was to reveal the decomposition dynamics of kiwifruit litter and verify the variety differences and provide a scientific basis for rational fertilization in orchard. Kiwifruit litters of two varieties (‘Hongyang’ and ‘Jinyan’) were taken as the objects; the litter decomposition rate, the dynamics of macro-elements and micro-elements, and soil enzyme activities during the decomposition process were analyzed. The results showed that the litter decomposition rate of ‘Hongyang’ kiwifruit was faster than that of ‘Jinyan’ kiwifruit, because of the higher initial N and P content in the litter of the ‘Hongyang’ kiwifruit. The dynamic trends of macro-elements and micro-elements during litter decomposition of two varieties were similar. The C content was relatively stable, the P content was fluctuant, and the K content was decreasing. The contents of N, Fe, Mn, Cu, and Zn were increasing. The contents of Ca, Mg, and B increased first and then decreased. After 180 days of the decomposition experiment, more than 75% of the initial contents of C, N, P, K, Ca, Mg, and B were released. The dynamic trends of the soil enzyme activities of two varieties were generally similar. Due to the slow decomposition rate, the dynamic trends of soil enzyme activities of ‘Jinyan’ kiwifruit litter each showed hysteresis. The contents of Ca, Mg, and Mn were significantly correlated with some soil enzyme activities. In conclusion, the litter substrate quality of the two kiwifruit varieties is different, which leads to the difference in the decomposition rate. The litter decomposition of kiwifruit is an important supplement to the macro-element in orchard soil.
The Method and Experiment of Kinetic Determination for the Rotary Soil-Engaging Components of Agricultural Machinery Using a Compacting Device in a Paddy Field as an Example
Authors: Wang Jinwu , Xiang Yushun , Wang Changyu , Xu Changsu , Zhu Guixuan , Gu Zejun , Wang Jinfeng , Tang Han
Published: March 2023

In order to explore the influence of soil force on rotary soil-engaging components during their operation, a kinetic parameter measurement system of rotary soil-engaging components was developed via the sensor strain measurement technique. By taking a single-sided compacting ridge device as an example, the kinetic experiment was carried out on the operation parameters of the compacting device and obtained the variation law of the force of the soil on the pinnae. ADAMS software was used to simulate the operation of the compacting device under different experimental factors (soil moisture content, forward speed of the ridge device, and the rotation speed of the compacting device), and the test data were obtained; the experiment was carried out with the forward speed of the soil tanker and the rotation speed of the compacting device as the experimental factors, and the average of the force of soil on the pinnae and the firmness value of the ridge were the experimental indicators. The results showed that under a moisture content of 27%, the forward speed of the device was 0.8 km∙h−1, the maximum firmness of the ridge was 1233.21 kPa, the minimum firmness of the ridge was 953.85 kPa, and the coefficient of variation of the stability of the firmness value of the ridge was 8.04; the force of the pinnae on the soil increased with an increase in the forward speed of the soil tanker, and the variation range of the force was 2838.1–5695.2 N. It was verified that the design of this operation parameter measurement system for rotary soil-engaging components meets the practical requirements and also provides an important reference for the measurement of the relevant parameters of similar rotary soil-engaging components.
Spatial Variation in Soil Base Saturation and Exchangeable Cations in Tropical and Subtropical China
Authors: Zhang Jing , Qu Xiaolin , Song Xiaodong , Xiao Ying , Wang Anqi , Li Decheng
Published: March 2023

In the last 30 years, severe soil acidification has been found in China due to acid deposition and nitrogen fertilizer overuse. Understanding the spatial pattern and vertical variations in base saturation percentage (BSP) and exchangeable cations (Ca2+, Mg2+, K+, Na+, H+ and Al3+) can directly benefit fertilization management and ecological protection. Here, 1253 soil profiles were surveyed in tropical and subtropical regions in China to investigate the spatial variations in BSP and exchangeable cations at three soil depths of 0–20 cm, 20–50 cm and 50–100 cm. The spatial distributions were interpolated by using advanced machine learning techniques. We found that the exchangeable Ca2+ (Exch. Ca), Mg2+ (Exch. Mg) and BSP were significantly higher in paddy fields and uplands than in forests and gardens, regardless of soil depth, while the exchangeable K (Exch. K) did not significantly differ between various land-use types. The Exch. Ca and BSP in Anthrosols were significantly higher than those in Ferrosols, Argosols and Cambosols in the three soil layers. The spatial prediction results indicated that exchangeable cations and BSP were generally characterized by strong heterogeneity, and the Exch. Ca, Exch. K and exchangeable H+ (Exch. H) contents and BSP declined with increasing soil depth. This study helps us understand the spatial variation in BSP and exchangeable cations in the study area and benefits fertilization management and environmental protection.
Efficacy and Safety of Oral Probiotics in Children with Allergic Rhinitis: A Review
Authors: Klain Angela , Dinardo Giulio , Indolfi Cristiana , Contieri Marcella , Salvatori Alessandra , Vitale Sossio , Decimo Fabio , Ciprandi Giorgio , Miraglia del Giudice Michele
Published: March 2023

The prevalence of allergic rhinitis is rising, and it is impacting children’s growth and quality of life. To uncover unconventional treatment modalities, research was carried out to clarify the significance of novel components in the pathophysiology of allergic rhinitis. One of these elements was gut microbiota, which plays a crucial role in the development and evolution of allergic disorders. Specifically, dysbiosis, defined as impaired microbiota composition, characterizes allergic disorders. In light of this concept, probiotics (beneficial bacteria) may restore gut dysbiosis, rebalance the immune response, and indirectly influence the clinical course of allergic diseases. In this article, we discussed the role of the gut–lung axis in children and reported on new findings. We also reviewed the most relevant studies about probiotics in patients with allergic rhinitis.
Measuring Sheep Tails: A Preliminary Study Using Length (Mm), Vulva Cover Assessment, and Number of Tail Joints
Authors: Woodruff Madeleine , Munoz Carolina , Coleman Grahame , Doyle Rebecca , Barber Stuart
Published: March 2023

Docking sheep tails is a long-standing practice that, when done at the recommended length, reduces the risk of flystrike. The recommended length is to cover the vulva of ewes and to a similar length in males. This length is often equated to three coccygeal joints left intact, and there are many other ways the recommended length is described by researchers, industry, and government. This study compared the observer consistency and retest consistency using three different tail length measurement methods: vulva cover assessment, length (mm), and joint palpation. The tails of 51 yearling and 48 weaner Merino ewes were assessed by two observers. Length and vulva cover assessment methods provided the most reliable results, and joint palpation was the least reliable method of tail measurement. In the sample, tails that covered the vulva of yearlings and weaners measured 57.6 mm (n = 14) and 63.7 mm (n = 30) on average, respectively, and contained two coccygeal joints (more than two coccygeal vertebrae). Tails that did not cover the vulva of yearlings and weaners measured 41.3 mm (n = 36) and 52.8 mm (n = 17) on average, respectively, and had less than two coccygeal joints. The two most reliable methods enable valid comparison to the best practice recommendations.
Effect of Arabinoxylan and Xylo-Oligosaccharide on Growth Performance and Intestinal Barrier Function in Weaned Piglets
Authors: Sun Feize , Li Huahui , Sun Zhiqiang , Liu Ling , Zhang Xiujun , Zhao Jinbiao
Published: March 2023

The purpose of this study was to explore the effects of xylose with different polymerizations on growth performance, intestinal barrier function, and gut microbial composition in weaned piglets. A total of 144 weaned piglets were assigned to 3 dietary treatments in a completely randomized design according to their body weight and sex. Dietary treatments included a corn-soybean meal basal diet (CON) and 2 additional diets formulated with 1% arabinoxylan (AX) and 1% xylo-oligosaccharide (XOS), respectively. Results showed that dietary supplementation of XOS or AX reduced diarrhea incidence of weaned piglets compared with the CON group (p < 0.05). XOS or AX increased the ileal villus height and intestinal activity of antioxidases in weaned piglets compared with the CON group (p < 0.05). XOS or AX reduced the ileal and colonic IL-6 content and increased the colonic sIgA and IL-10 concentrations in weaned piglets compared with the CON group (p < 0.05). XOS or AX increased the total organic acids concentrations in the ileum and in vitro fermentation (p < 0.05). XOS increased the abundance of Lactobacillus and Bifidobacterium in the ileal digesta (p < 0.05), while AX increased the population of Lactobacillus in the ileal digesta and the abundance of Bifidobacterium in the colonic digesta of weaned piglets (p < 0.05). In conclusion, both XOS and AX reduce diarrhea incidence and improve antioxidant capacity, immune function, and populations of beneficial bacteria, while microbial fermentation of XOS with a lower polymerization and molecular mass can produce more organic acids and an increased abundance of Lactobacillus and Bifidobacterium in the upper gut of weaned pigs compared with AX.
Antimicrobial Resistance in Physiological and Potentially Pathogenic Bacteria Isolated in Southern Italian Bats
Authors: Foti Maria , Grasso Rosario , Fisichella Vittorio , Mascetti Antonietta , Colnaghi Marco , Grasso Maria , Spena Maria Teresa
Published: March 2023

The spread of antimicrobial resistance is one of the major health emergencies of recent decades. Antimicrobial-resistant bacteria threaten not only humans but also populations of domestic and wild animals. The purpose of this study was to evaluate the distribution of antibiotic resistance (AMR) and multidrug resistance (MDR) in bacterial strains isolated from six Southern-Italian bat populations. Using the disk diffusion method, we evaluated the antimicrobial susceptibility of 413 strains of Gram-negative bacteria and 183 strains of Gram-positive bacteria isolated from rectal (R), oral (O) and conjunctival (C) swabs of 189 bats belonging to 4 insectivorous species (Myotis capaccinii, Myotis myotis, Miniopterus schreibersii and Rhinolophus hipposideros). In all bat species and locations, numerous bacterial strains showed high AMR levels for some of the molecules tested. In both Gram-negative and Gram-positive strains, the resistance patterns ranged from one to thirteen. MDR patterns varied significantly across sites, with Grotta dei Pipistrelli in Pantalica displaying the highest levels of MDR (77.2% of isolates). No significant differences were found across different bat species. Monitoring antibiotic resistance in wildlife is a useful method of evaluating the impact of anthropic pressure and environmental pollution. Our analysis reveals that anthropic contamination may have contributed to the spread of the antibiotic resistance phenomenon among the subjects we examined.
Physiological Response of Grower African Catfish to Dietary Black Soldier Fly and Mealworm Meal
Authors: Gebremichael Askale , Kucska Balázs , Ardó László , Biró Janka , Berki Mária , Lengyel-Kónya Éva , Tömösközi-Farkas Rita , Egessa Robert , Müller Tamás , Gyalog Gergő , Sándor Zsuzsanna J.
Published: March 2023

A six-week experiment was carried out to test the effects of total (100%) and partial (50%) replacement of fish meal in the diet of African catfish growers with black soldier fly (B) meal, yellow mealworm (M) meal, and a 1:1 combination of both (BM) on the production and health of fish. A total of 420 fish with an average initial body weight of 200 ± 0.5 g were randomly distributed in triplicate to seven diet groups (C, B50, B100, M50, M100, BM50, and BM100, respectively). The growth performance and feed utilization of fish fed with partial or total replacement levels of FM with B were not significantly affected (p > 0.05) during the 6 weeks of feeding. In contrast, significant differences were observed between the groups fed with a diet where FM was totally replaced with M meal and the control in terms of final body weight, specific growth rate, feed conversion ratio, protein efficiency ratio, and protein productive value. Among the blood plasma biochemistry parameters, total cholesterol exhibited a significant difference (p = 0.007) between the M treatments and the control diet. The fatty acid profile of the liver was changed with respect to the long-chain polyunsaturated fatty acid content in all experimental groups. Parallel with this, the upregulation of elovl5 and fas genes in liver was found in all experimental groups compared to the control. Overall, this study shows that fish meal cannot be substituted with yellow mealworm meal in the practical diet of African catfish without compromising the growth, health and feed utilization parameters.
Paired Analysis of D-Dimer and Its Correlated Hemostatic Parameters in 30 Dogs with Neoplasms after Tumorectomy
Authors: Ke Chiao-Hsu , Liu Cheng-Chi , Wang Shang-Lin , Lin Chen-Si
Published: March 2023

Previous studies have reported that dogs with neoplasms had elevated D-dimer levels. However, few studies have addressed whether D-dimer could be an indicator of tumor burden. The clinical significance of paired analysis of pre- and post-operation of D-dimer levels in dogs has rarely been described. The present study investigated the values of D-dimer levels and their correlated hemostatic alterations in dogs with surgically removable benign and malignant tumors. This study analyzed 30 clinically healthy and 30 tumor-bearing dogs and evaluated the hemostatic functions including D-dimer, thromboelastography G (TEG G), fibrinogen, activated partial thromboplastin time (aPTT), prothrombin time, and platelet count. The median level of pre-treatment D-dimer was 0.8 µg/mL (range: 0.1–6.3 µg/mL), whereas the control dogs exhibited a median value of 0.1 µg/mL (range: 0.1–0.1 µg/mL, p < 0.0001). After tumorectomy, the median levels of D-dimer (p < 0.0001), fibrinogen (p < 0.0001), TEG G value (p < 0.01), and aPTT (p < 0.05) were significantly lower than those of the pre-treatment samples. However, further studies are needed to clarify the values of other hemostatic evaluations. The study revealed the clinical significance of D-dimer and its correlated hemostatic parameters by paired analysis in dogs with tumors. Though more cases are needed for solid confirmation, these values could be potential tumor biomarkers for dogs.
Comparative Analysis of Olfactory Receptor Repertoires Sheds Light on the Diet Adaptation of the Bamboo-Eating Giant Panda Based on the Chromosome-Level Genome
Authors: Zhou Chuang , Liu Yi , Zhao Guangqing , Liu Zhengwei , Chen Qian , Yue Bisong , Du Chao , Zhang Xiuyue
Published: March 2023

The giant panda (Ailuropoda melanoleuca) is the epitome of a flagship species for wildlife conservation and also an ideal model of adaptive evolution. As an obligate bamboo feeder, the giant panda relies on the olfaction for food recognition. The number of olfactory receptor (OR) genes and the rate of pseudogenes are the main factors affecting the olfactory ability of animals. In this study, we used the chromosome-level genome of the giant panda to identify OR genes and compared the genome sequences of OR genes with five other Ursidae species (spectacled bear (Tremarctos ornatus), American black bear (Ursus americanus), brown bear (Ursus arctos), polar bear (Ursus maritimus) and Asian black bear (Ursus thibetanus)). The giant panda had 639 OR genes, including 408 functional genes, 94 partial OR genes and 137 pseudogenes. Among them, 222 OR genes were detected and distributed on 18 chromosomes, and chromosome 8 had the most OR genes. A total of 448, 617, 582, 521 and 792 OR genes were identified in the spectacled bear, American black bear, brown bear, polar bear and Asian black bear, respectively. Clustering analysis based on the OR protein sequences of the six species showed that the OR genes distributed in 69 families and 438 subfamilies based on sequence similarity, and the six mammals shared 72 OR gene subfamilies, while the giant panda had 31 unique OR gene subfamilies (containing 35 genes). Among the 35 genes, there are 10 genes clustered into 8 clusters with 10 known human OR genes (OR8J3, OR51I1, OR10AC1, OR1S2, OR1S1, OR51S1, OR4M1, OR4M2, OR51T1 and OR5W2). However, the kind of odor molecules can be recognized by the 10 known human OR genes separately, which needs further research. The phylogenetic tree showed that 345 (about 84.56%) functional OR genes were clustered as Class-II, while only 63 (about 15.44%) functional OR genes were clustered as Class-I, which required further and more in-depth research. The potential odor specificity of some giant panda OR genes was identified through the similarity to human protein sequences. Sequences similar to OR2B1, OR10G3, OR11H6 and OR11H7P were giant panda-specific lacking, which may be related to the transformation and specialization from carnivore to herbivore of the giant panda. Since our reference to flavoring agents comes from human research, the possible flavoring agents from giant panda-specific OR genes need further investigation. Moreover, the conserved motifs of OR genes were highly conserved in Ursidae species. This systematic study of OR genes in the giant panda will provide a solid foundation for further research on the olfactory function and variation of the giant panda.
Bone Morphogenetic Protein 15 (BMP-15) Improves In Vitro Mouse Folliculogenesis
Authors: Jitjumnong Jakree , Tang Pin-Chi
Published: March 2023

Multilayered secondary follicles were encapsulated in a 0.5% alginate matrix and cultured in a 3D culture system supplemented with bone morphogenetic protein 15 (BMP-15; 15 ng/mL) for 12 days. The in vitro development of ovarian follicles was evaluated. On day 12, the follicle diameter, follicle survival rate, and antrum formation rate were significantly higher for follicles cultured in BMP-15-supplemented medium than those cultured in regular medium. The percentage of ovulated metaphase II oocytes retrieved from follicles cultured in BMP-15-supplemented medium was greater than that of oocytes retrieved from follicles cultured in regular medium. The secretion of P4 was significantly higher on days 6, 8, and 10 in follicles cultured in BMP-15-supplemented medium. The result for E2 tended toward significance on day 12. Intracellular reactive oxygen species levels were higher and glutathione levels were lower in mature oocytes from the in vitro culture than in mature oocytes from an in vivo control. A 3D culture system using an alginate matrix and supplemented with BMP-15 effectively improves the outcomes of in vitro ovarian follicle culture.
Methacrylate Cationic Nanoparticles Activity against Different Gram-Positive Bacteria
Authors: Nam-Cha Syong H. , Ocaña Ana V. , Pérez-Tanoira Ramón , Aguilera-Correa John J. , Domb Abraham J. , Ruiz-Grao Marta C. , Cebada-Sánchez Sandra , López-Gónzalez Ángel , Molina-Alarcón Milagros , Pérez-Martínez Juan , Pérez-Martínez Francisco C.
Published: March 2023

Nanotechnology is a developing field that has boomed in recent years due to the multiple qualities of nanoparticles (NPs), one of which is their antimicrobial capacity. We propose that NPs anchored with 2-(dimethylamino)ethyl methacrylate (DMAEMA) have antibacterial properties and could constitute an alternative tool in this field. To this end, the antimicrobial effects of three quaternised NPs anchored with DMAEMA were studied. These NPs were later copolymerized using different methylmethacrylate (MMA) concentrations to evaluate their role in the antibacterial activity shown by NPs. Clinical strains of Staphylococcus aureus, S. epidermidis, S. lugdunensis and Enterococcus faecalis were used to assess antibacterial activity. The minimal inhibitory concentration (MIC) was determined at the different concentrations of NPs to appraise antibacterial activity. The cytotoxic effects of the NPs anchored with DMAEMA were determined in NIH3T3 mouse fibroblast cultures by MTT assays. All the employed NPs were effective against the studied bacterial strains, although increasing concentrations of the MMA added during the synthesis process diminished these effects without altering toxicity in cell cultures. To conclude, more studies with other copolymers are necessary to improve the antibacterial effects of NPs anchored with DMAEMA.
Antimicrobial Therapy Duration for Bloodstream Infections Caused by Pseudomonas aeruginosa or Acinetobacter baumannii-calcoaceticus complex: A Retrospective Cohort Study
Authors: Rodrigues Rodrigo Douglas , Garcia Rebeca Carvalho Lacerda , Bittencourt Gabriel Almeida , Waichel Vicente Bouchet , Garcia Ester Carvalho Lacerda , Rigatto Maria Helena
Published: March 2023

Background: Ideal therapy duration for Pseudomonas aeruginosa or Acinetobacter baumannii-calcoaceticus complex (ABC) bloodstream infections (BSI) is not defined, especially in the context of carbapenem resistance. In this study, we compared short- (≤7 days) and long-term (>7 days) antimicrobial therapy duration for these infections. Methods: We performed a retrospective cohort study in two tertiary-care hospitals in Porto Alegre, Brazil, from 2013 to 2019. Eligible patients aged ≥18 years were included and excluded for the following criteria: polymicrobial infections, treatment with non-susceptible antibiotics, complicated infections, or early mortality (<8 days of active antimicrobial therapy). The 30-day mortality risk was evaluated using a Cox regression model. Results: We included 237 BSI episodes, 51.5% caused by ABC and 48.5% by Pseudomonas aeruginosa. Short-term therapy was not associated with 30-day mortality, adjusted hazard ratio 1.01, 95% confidence interval 0.47–2.20, p = 0.98, when adjusted for Pitt score (p = 0.02), Charlson Comorbidity Index score (p < 0.01), and carbapenem resistance (p < 0.01). Among patients who survived, short-term therapy was associated with shorter hospital stay (p < 0.01). Results were maintained in the subgroups of BSI caused by carbapenem-resistant bacteria (p = 0.76), ABC (p = 0.61), and Pseudomonas aeruginosa (p = 0.39). Conclusions: Long-term therapies for non-complicated Pseudomonas aeruginosa and ABC BSI were not superior to short-term therapy for 30-day mortality.
Accumulation of Proline in Plants under Contaminated Soils—Are We on the Same Page?
Authors: Spormann Sofia , Nadais Pedro , Sousa Filipa , Pinto Mafalda , Martins Maria , Sousa Bruno , Fidalgo Fernanda , Soares Cristiano
Published: March 2023

Agricultural soil degradation is occurring at unprecedented rates, not only as an indirect effect of climate change (CC) but also due to intensified agricultural practices which affect soil properties and biodiversity. Therefore, understanding the impacts of CC and soil degradation on plant physiology is crucial for the sustainable development of mitigation strategies to prevent crop productivity losses. The amino acid proline has long been recognized for playing distinct roles in plant cells undergoing osmotic stress. Due to its osmoprotectant and redox-buffering ability, a positive correlation between proline accumulation and plants’ tolerance to abiotic stress has been pointed out in numerous reviews. Indeed, proline quantification is used systematically by plant physiologists as an indicator of the degree of tolerance and a measurement of the antioxidant potential in plants under stressful conditions. Moreover, the exogenous application of proline has been shown to increase resilience to several stress factors, including those related to soil degradation such as salinity and exposure to metals and xenobiotics. However, recent data from several studies often refer to proline accumulation as a signal of stress sensitivity with no clear correlation with improved antioxidant activity or higher stress tolerance, including when proline is used exogenously as a stress reliever. Nevertheless, endogenous proline levels are strongly modified by these stresses, proving its involvement in plant responses. Hence, one main question arises—is proline augmentation always a sign of improved stress resilience? From this perspective, the present review aims to provide a more comprehensive understanding of the implications of proline accumulation in plants under abiotic stress induced by soil degradation factors, reinforcing the idea that proline quantification should not be employed as a sole indicator of stress sensitivity or resilience but rather complemented with further biochemical and physiological endpoints.
Receiver Operating Characteristic Analysis of Posture and Gait Parameters to Prevent Frailty Condition and Fall Risk in the Elderly
Authors: Presta Valentina , Galuppo Laura , Condello Giancarlo , Rodà Francesca , Mirandola Prisco , Vitale Marco , Vaccarezza Mauro , Gobbi Giuliana
Published: March 2023

Prevention strategies should be constantly improved to manage falls and frailty in the elderly. Therefore, we aimed at creating a screening and predictive protocol as a replicable model in clinical settings. Bioimpedance analysis was conducted on fifty subjects (mean age 76.9 ± 3.69 years) to obtain body composition; then, posture was analysed with a stabilometric platform. Gait performance was recorded by a 10 m walking test, six-minute walking test, and timed up and go test. After 12 months, subjects were interviewed to check for fall events. Non-parametric analysis was used for comparisons between fallers and non-fallers and between able and frail subjects. ROC curves were obtained to identify the predictive value of falling risk and frailty. Path length (area under the curve, AUC = 0.678), sway area (AUC = 0.727), and sway speed (AUC = 0.778) resulted predictive factors of fall events (p < 0.05). The six-minute walking test predicted frailty condition (AUC = 0.840). Timed up and go test was predictive of both frailty (AUC = 0.702) and fall events (AUC = 0.681). Stabilometry and gait tests should be, therefore, included in a screening protocol for the elderly to prevent fall events and recognize the condition of frailty at an early stage.