bluuebunny/crossref_metadata_2025 huggingface dataset:
Supply Chain Coordination under Carbon Emission Tax Regulation Considering Greening Technology Investment
Authors: Wei Zhimin , Huang Yun
Published: July 2022

In this paper, we firstly derive the optimal strategies, including greening technology investment, production volume and order quantity decisions with stochastic demand, for the emissions-dependent supply chain composed of one manufacturer and one retailer. Then, an advance purchase discount (APD) contract and an option contract are applied to coordinate the supply chain. Moreover, an innovative prepayment-based option (PBO) contract is designed based on an APD contract and an option contract. We discuss the cash flow, the inventory risk allocation and the impacts of carbon emission tax under each contract. It is found that considering improving cash flow, preselling (or option selling) as a means of supporting the manufacturer with sufficient cash flow will help expand production and invest in greening technology. From the perspective of avoiding inventory risk, the APD contract benefits the manufacturer while the option contract benefits the retailer. However, the PBO contract generates intermediate allocations of inventory risk between manufacturer and retailer.
Fostering Nursing Staff Competence in Personal Protective Equipment Education during COVID-19: A Mobile-Video Online Learning Approach
Authors: Jen Hsiu-Ju , Chou Kuei-Ru , Chang Ching-Yi
Published: July 2022

Nursing staff who are competent to use personal protective equipment (PPE) correctly can protect themselves while providing safe, high-quality care to patients. Under pandemic conditions, the ability to wear PPE correctly is essential in clinical practice, but the acquisition of correct PPE-wearing procedures is difficult for most staff in the absence of live practice drills. This study aimed to test the mobile video online learning approach by integrating PPE contexts into a digital learning system. We conducted an experiment to verify whether the mobile video online learning approach could effectively improve nursing staff’s learning achievement, learning anxiety, critical thinking skills, and learning self-efficacy. The study used a quasi-experimental design and was conducted with 47 nursing staff, divided into one group using a mobile video online learning approach and one group with a conventional learning approach. We used pre-and post-test examinations of learning achievements, learning anxiety, critical thinking, and learning self-efficacy. Results showed a significant effect of using the mobile video online learning method in helping nursing staff to decrease learning anxiety and improve knowledge about COVID-19 protection, increase learning achievement, critical thinking skills, and learning self-efficacy. These benefits are of interest to nursing workplace managers wishing to maintain professional standards during epidemics by improving the nursing staff’s PPE knowledge and self-efficacy concerning PPE.
SARS-CoV-2 Delta AY.1 Variant Cluster in an Accommodation Facility for COVID-19: Cluster Report
Authors: Ohishi Takayuki , Yamagishi Takuya , Kurosu Hitomi , Kato Hideaki , Takayama Yoko , Anan Hideaki , Kunishima Hiroyuki
Published: July 2022

Background: This study aimed to examine the cause of and effective measures against cluster infections, including the delta AY.1 variant of novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that occurred in an accommodation facility. Methods: We surveyed the zoning and ventilation systems of the cluster accommodation, examined the staff’s working conditions, conducted an interview, and administered a SARS-CoV-2 test (positive samples were further tested with molecular biological test). Results: Among the 99 employees working at the accommodation, 10 were infected with the delta AY.1 variant. The causes of the cluster infections were close-distance conversations without an unwoven-three-layer mask and contact for approximately five minutes with an unwoven mask under hypoventilated conditions. Conclusions: The Delta AY.1 infection may occur via aerosols and an unwoven mask might not prevent infection in poorly ventilated small spaces. Routine infection detection and responding quickly and appropriately to positive results helps to prevent clusters from spreading.
Numerical Study on the Influence of Model Uncertainties on the Transport of Underwater Spilled Oil
Authors: Wang Daosheng , Luo Zhixuan , Mu Lin
Published: July 2022

Oil pollution influences marine biology, ecology, and regional sustainable development capacity, but model uncertainties limit the ability of the numerical model to accurately predict the transport and fate of the underwater oil spill. Based on a three-dimensional underwater oil spill model validated by satellite images of the oil slick at the sea surface, the Penglai 19-3 oil spill accident in the Bohai Sea was simulated; in addition, several sensitivity experiments were set up to investigate the influence of model uncertainties in the background wind, current, start time of the oil spill, and spill site on the transport of underwater spilled oil in the Penglai 19-3 oil spill accident. The experimental results indicate that the uncertainty in the background wind has a certain impact on the simulated centroid position at the sea surface, and little effect on the simulated underwater results, while the uncertainty in the background current has a significant influence on the transport of the underwater spilled oil both at the sea surface and underwater. An uncertainty of 24 h in the start time of the oil spill can cause more than 1 time larger than the benchmark case displacement of the oil spill centroid point and sweeping area at the sea surface, as the periodic tidal current is the main constituent of the ocean current in the Bohai Sea. The uncertainty in the spill site has a large influence on the final position of the oil spill centroid point, but the oil spill trajectories do not intersect with each other within 48 h, which makes it possible to identify the oil spill platform from the actual observations. The influence of uncertainties in the important model inputs and key model parameters on the transport of underwater spilled oil in the Penglai 19-3 oil spill accident is evaluated for the first time, which is of substantial significance for improving the prediction accuracy of the transport and fate of underwater oil spills.
Platelet Lipidome Fingerprint: New Assistance to Characterize Platelet Dysfunction in Obesity
Authors: Chicanne Gaëtan , Barrachina Maria N. , Durbec Anaelle , Bertrand-Michel Justine , Troitiño Sara , Hermida-Nogueira Lidia , Sueiro Aurelio M. , Pardo María , Payrastre Bernard , García Ángel
Published: July 2022

Obesity is associated with a pro-inflammatory and pro-thrombotic state that supports atherosclerosis progression and platelet hyper-reactivity. During the last decade, the platelet lipidome has been considered a treasure trove, as it is a source of biomarkers for preventing and treating different pathologies. The goal of the present study was to determine the lipid profile of platelets from non-diabetic, severely obese patients compared with their age- and sex-matched lean controls. Lipids from washed platelets were isolated and major phospholipids, sphingolipids and neutral lipids were analyzed either by gas chromatography or by liquid chromatography coupled to mass spectrometry. Despite a significant increase in obese patient’s plasma triglycerides, there were no significant differences in the levels of triglycerides in platelets among the two groups. In contrast, total platelet cholesterol was significantly decreased in the obese group. The profiling of phospholipids showed that phosphatidylcholine and phosphatidylethanolamine contents were significantly reduced in platelets from obese patients. On the other hand, no significant differences were found in the sphingomyelin and ceramide levels, although there was also a tendency for reduced levels in the obese group. The outline of the glycerophospholipid and sphingolipid molecular species (fatty-acyl profiles) was similar in the two groups. In summary, these lipidomics data indicate that platelets from obese patients have a unique lipid fingerprint that may guide further studies and provide mechanistic-driven perspectives related to the hyperactivate state of platelets in obesity.
The Gestational Effects of Maternal Bone Marker Molecules on Fetal Growth, Metabolism and Long-Term Metabolic Health: A Systematic Review
Authors: Dimas Angelos , Politi Anastasia , Bargiota Alexandra , Panoskaltsis Theodoros , Vlahos Nikolaos F. , Valsamakis Georgios
Published: July 2022

Fetal exposure in adverse environmental factors during intrauterine life can lead to various biological adjustments, affecting not only in utero development of the conceptus, but also its later metabolic and endocrine wellbeing. During human gestation, maternal bone turnover increases, as reflected by molecules involved in bone metabolism, such as vitamin D, osteocalcin, sclerostin, sRANKL, and osteoprotegerin; however, recent studies support their emerging role in endocrine functions and glucose homeostasis regulation. Herein, we sought to systematically review current knowledge on the effects of aforementioned maternal bone biomarkers during pregnancy on fetal intrauterine growth and metabolism, neonatal anthropometric measures at birth, as well as on future endocrine and metabolic wellbeing of the offspring. A growing body of literature converges on the view that maternal bone turnover is likely implicated in fetal growth, and at least to some extent, in neonatal and childhood body composition and metabolic wellbeing. Maternal sclerostin and sRANKL are positively linked with fetal abdominal circumference and subcutaneous fat deposition, contributing to greater birthweights. Vitamin D deficiency correlates with lower birthweights, while research is still needed on intrauterine fetal metabolism, as well as on vitamin D dosing supplementation during pregnancy, to diminish the risks of low birthweight or SGA neonates in high-risk populations.
Sirtuin 3 Deficiency Aggravates Kidney Disease in Response to High-Fat Diet through Lipotoxicity-Induced Mitochondrial Damage
Authors: Locatelli Monica , Macconi Daniela , Corna Daniela , Cerullo Domenico , Rottoli Daniela , Remuzzi Giuseppe , Benigni Ariela , Zoja Carlamaria
Published: July 2022

Sirtuin 3 (SIRT3) is the primary mitochondrial deacetylase that controls the antioxidant pathway and energy metabolism. We previously found that renal Sirt3 expression and activity were reduced in mice with type 2 diabetic nephropathy associated with oxidative stress and mitochondrial abnormalities and that a specific SIRT3 activator improved renal damage. SIRT3 is modulated by diet, and to assess whether Sirt3 deficiency aggravates mitochondrial damage and accelerates kidney disease in response to nutrient overloads, wild-type (WT) and Sirt3−/− mice were fed a high-fat-diet (HFD) or standard diet for 8 months. Sirt3−/− mice on HFD exhibited earlier and more severe albuminuria compared to WT mice, accompanied by podocyte dysfunction and glomerular capillary rarefaction. Mesangial matrix expansion, tubular vacuolization and inflammation, associated with enhanced lipid accumulation, were more evident in Sirt3−/− mice. After HFD, kidneys from Sirt3−/− mice showed more oxidative stress than WT mice, mitochondria ultrastructural damage in tubular cells, and a reduction in mitochondrial mass and energy production. Our data demonstrate that Sirt3 deficiency renders mice more prone to developing oxidative stress and mitochondrial abnormalities in response to HFD, resulting in more severe kidney diseases, and this suggests that mitochondria protection may be a method to prevent HFD-induced renal injury.
Multigenerational Exposure to Uranium Changes Sperm Metabolome in Rats
Authors: Grison Stéphane , Legendre Audrey , Svilar Ljubica , Elie Christelle , Kereselidze Dimitri , Gloaguen Céline , Lestaevel Philippe , Martin Jean-Charles , Souidi Maâmar
Published: July 2022

Male infertility is a major public health issue that can be induced by a host of lifestyle risk factors such as environment, nutrition, smoking, stress, and endocrine disruptors. Regarding the human population exposed to uranium, it is necessary to explore these effects on male reproduction in multigenerational studies. The sensitivity of mass spectrometry (MS)-based methods has already proved to be extremely useful in metabolite identification in rats exposed to low doses of uranium, but also in human sperm. We applied this method to rat sperm over three generations (F0, F1 and F2) with multigenerational uranium exposure. Our results show a significant content of uranium in generation F0, and a reduction in the pregnancy rate only in generation F1. Based on principal component analysis (PCA), we observed discriminant profiles between generations. The partial least squares discriminant analysis (PLS-DA) of the 48 annotated variables confirmed that parental exposure of generation F0 (during both the preconceptional and prenatal periods) can have metabolic effects on spermatozoa for the next two generations. Metabolomics applied to epididymal spermatozoa is a novel approach to detecting the multigenerational effects of uranium in an experimental model, but could be also recommended to identify potential biomarkers evaluating the impact of uranium on sperm in exposed infertile men.
GTP Binding Protein Gtr1 Cooperating with ASF1 Regulates Asexual Development in Stemphylium eturmiunum
Authors: Wang Shi , Song Chunyan , Zhao Lili , Xu Wenmeng , Li Zhuang , Liu Xiaoyong , Zhang Xiuguo
Published: July 2022

The Gtr1 protein was a member of the RagA subfamily of the Ras-like small GTPase superfamily and involved in phosphate acquisition, ribosome biogenesis and epigenetic control of gene expression in yeast. However, Gtr1 regulation sexual or asexual development in filamentous fungi is barely accepted. In the study, SeGtr1, identified from Stemphylium eturmiunum, could manipulate mycelial growth, nuclear distribution of mycelium and the morphology of conidia in Segtr1 silenced strains compared with its overexpression transformants, while the sexual activity of Segtr1 silenced strains were unchanged. SeASF1, a H3/H4 chaperone, participated in nucleosome assembly/disassembly, DNA replication and transcriptional regulation. Our experiments showed that deletion Seasf1 mutants produced the hyphal fusion and abnormal conidia. Notably, we characterized that Segtr1 was down-regulated in Se∆asf1 mutants and Seasf1 was also down-regulated in SiSegtr1 strains. We further confirmed that SeGtr1 interacted with SeASF1 or SeH4 in vivo and vitro, respectively. Thus, SeGtr1 can cooperate with SeASF1 to modulate asexual development in Stemphylium eturmiunum.
Cuprous Oxide Thin Films Implanted with Chromium Ions—Optical and Physical Properties Studies
Authors: Ungeheuer Katarzyna , Marszalek Konstanty W. , Mitura-Nowak Marzena , Jelen Piotr , Perzanowski Marcin , Marszalek Marta , Sitarz Maciej
Published: July 2022

Cuprous oxide is a semiconductor with potential for use in photocatalysis, sensors, and photovoltaics. We used ion implantation to modify the properties of Cu2O oxide. Thin films of Cu2O were deposited with magnetron sputtering and implanted with low-energy Cr ions of different dosages. The X-ray diffraction method was used to determine the structure and composition of deposited and implanted films. The optical properties of the material before and after implantation were studied using spectrophotometry and spectroscopic ellipsometry. The investigation of surface topography was performed with atomic force microscopy. The implantation had little influence on the atomic lattice constant of the oxide structure, and no clear dependence of microstrain or crystalline size on the dose of implantation was found. The appearance of phase change was observed, which could have been caused by the implantation. Ellipsometry measurements showed an increase in the total thickness of the sample with an increase in the amount of implanted Cr ions, which indicates the influence of implantation on the properties of the surface and subsurface region. The refractive index n, extinction coefficient k, and absorption coefficient optical parameters show different energy dependences related to implantation dose.
Different In Vitro-Generated MUTZ-3-Derived Dendritic Cell Types Secrete Dexosomes with Distinct Phenotypes and Antigen Presentation Potencies
Authors: Sakamoto Takuya , Koya Terutsugu , Togi Misa , Yoshida Kenichi , Kato Tomohisa , Ishigaki Yasuhito , Shimodaira Shigetaka
Published: July 2022

Human dendritic cell (DC) dexosomes were evaluated for their function and preclinical validation for vaccines. Dexosomes are small DC-secreted vesicles that contain absorbing immune signals. Vaccine manufacturing requires a significant number of monocyte-derived DCs (Mo-DCs) from donor blood; thus, Mo-DC dexosomes are expected to serve as novel materials for cancer vaccination. In this study, we characterized a potential dexosome model using immature and mature MUTZ3-derived DCs (M-imIL-4-DC, M-imIFN-DC, M-mIL-4-DC, and M-mIFN-DC) and their dexosomes (M-imIL-4-Dex, M-imIFN-Dex, M-mIL4-Dex, and M-mIFN-Dex). Despite the lack of significant differences in viability, M-mIFN-DC showed a significantly higher level of yield and higher levels of maturation surface markers, such as CD86 and HLA-ABC, than M-mIL-4-DC. In addition, M-mIFN-Dex expressed a higher level of markers, such as HLA-ABC, than M-mIL-4-Dex. Furthermore, M-mIFN-Dex exhibited a higher level of antigen presentation potency, as evaluated using a MART-1 system, than either M-imIFN-Dex or M-mIL-4-Dex. We found that M-mIFN-Dex is one of the four types of MUTZ3-derived DCs that harbor potential immunogenicity, suggesting that DC dexosomes could be useful resources in cancer immunotherapy.
In Vivo and In Vitro Comparison of the DPP-IV Inhibitory Potential of Food Proteins from Different Origins after Gastrointestinal Digestion
Authors: Fleury Léa , Deracinois Barbara , Dugardin Camille , Nongonierma Alice B. , FitzGerald Richard J. , Flahaut Christophe , Cudennec Benoit , Ravallec Rozenn
Published: July 2022

Dipeptidyl-peptidase IV (DPP-IV) plays an essential role in glucose metabolism by inactivating incretins. In this context, food-protein-derived DPP-IV inhibitors are promising glycemic regulators which may act by preventing the onset of type 2 diabetes in personalized nutrition. In this study, the DPP-IV-inhibitory potential of seven proteins from diverse origins was compared for the first time in vitro and in vivo in rat plasma after the intestinal barrier (IB) passage of the indigested proteins. The DPP-IV-inhibitory potentials of bovine hemoglobin, caseins, chicken ovalbumin, fish gelatin, and pea proteins were determined in rat plasma thirty minutes after oral administration. In parallel, these proteins, together with bovine whey and gluten proteins, were digested using the harmonized INFOGEST protocol adapted for proteins. The DPP-IV half-maximal inhibitory concentration (IC50) was determined in situ using Caco-2 cells. The DPP-IV-inhibitory activity was also measured after IB passage using a Caco2/HT29-MTX mixed-cell model. The peptide profiles were analyzed using reversed-phase high-performance liquid chromatography tandem mass spectrometry (RP-HPLC-MS/MS) with MS data bioinformatics management, and the IC50 of the identified peptides was predicted in silico. The in vitro and in vivo DPP-IV-inhibitory activity of the proteins differed according to their origin. Vegetable proteins and hemoglobin yielded the highest DPP-IV-inhibitory activity in vivo. However, no correlation was found between the in vivo and in vitro results. This may be partially explained by the differences between the peptidome analysis and the in silico predictions, as well as the study complexity.
Cornerstones in Contemporary Inorganic Chemistry
Authors: Gregory Duncan H.
Published: July 2022

I am very happy to be able to present this Special Issue of Inorganics [...]
In Vitro Crude Protein Digestibility of Insects: A Review
Authors: Rodríguez-Rodríguez María , Barroso Fernando G. , Fabrikov Dmitri , Sánchez-Muros María José
Published: July 2022

The high protein content of insects has been widely studied. They can be a good food alternative, and therefore it is important to study the effect of digestion on their protein. This review examines the different in vitro protein digestibility methodologies used in the study of different edible insects in articles published up to 2021. The most important variables to be taken into account in in vitro hydrolysis are the following: phases (oral, gastric and intestinal), enzymes, incubation time and temperature, method of quantification of protein hydrolysis and sample preprocessing. Insects have high digestibility data, which can increase or decrease depending on the processing of the insect prior to digestion, so it is important to investigate which processing methods improve digestibility. The most commonly used methods are gut extraction, different methods of slaughtering (freezing or blanching), obtaining protein isolates, defatting, thermal processing (drying or cooking) and extrusion. Some limitations have been encountered in discussing the results due to the diversity of methodologies used for digestion and digestibility calculation. In addition, articles evaluating the effect of insect processing are very limited. It is concluded that there is a need for the standardisation of in vitro hydrolysis protocols and their quantification to facilitate comparisons in future research.
Special Issue: New Insights into the Pathogenesis and Therapies of IgA Nephropathy
Authors: Suzuki Hitoshi , Novak Jan
Published: July 2022

IgA nephropathy (IgAN) is the most common form of primary glomerulonephritis worldwide [...]
Robot-Assisted Tubal Reanastomosis after Sterilization: A Choice for Family Planning
Authors: Salehjawich Arwa , Günther Veronika , Ruchay Zino , Al Zoubi Mazhar Salim , Dhanawat Juhi , Maass Nicolai , Ackermann Johannes , Pape Julian , Alkatout Ibrahim
Published: July 2022

A variety of procedures have been used for family planning. One of these is sterilization surgery, which can be reversed by a tubal reanastomosis. In the present report, we compare Robot-assisted tubal reanastomosis sterilization with other methods of family planning and discuss factors related to the choice of the approach. The keywords used for the electronic search in PubMed were family planning, sterilization, Robot-assisted, tubal reanastomosis, depression, and regret. The decision in favor of or against sterilization surgery has been a sensitive issue for several years. Robot-assisted technology is a modern and precise approach. It has contributed to the flexibility of the decision between sterilization and its reversal through tubal reanastomosis, as well as enhanced the success rate of the surgery. Based on our analysis of the published literature, we believe that Robot-assisted tubal anastomosis is the optimum approach. However, to ensure the quality of health care, the surgeon must be well trained, well versed with the anatomy of the fallopian tubes, and thoroughly informed on the psychological impact of family planning.
Effect of Fetoscopic Laser Photocoagulation on Fetal Growth and Placental Perfusion in Twin–Twin Transfusion Syndrome
Authors: Chang Yao-Lung , Hsu Chin-Chieh , Chao An-Shine , Chang Shuenn-Dyh , Cheng Po-Jen , Li Wen-Fang
Published: July 2022

Objective: To investigate the fetal growth pattern after fetoscopic laser photocoagulation (FLP) in twin-twin transfusion syndrome (TTTS) and the effect of FLP on placental perfusion and intrauterine growth restriction (IUGR) incidence. Methods: TTTS cases with a live delivery of both twins at least 28 days after FLP and with a neonatal follow-up at our hospital at least 60 days after delivery were included. The biometric data obtained before FLP (based on ultrasound); time point M1), upon birth (M2), and at neonatal follow-up (M3) were analyzed. The body weight discordance (BWD) was defined as (estimated fetal weight [body weight] of the recipient twin − estimated fetal weight [body weight] of the donor twin)/(estimated fetal weight [body weight] of the recipient twin) × 100%. Total weight percentile (TWP) was defined as the donor + recipient twin weight percentile; the TWP indirectly reflected the total placental perfusion. Results: the BWDs decreased from M1 to M2 to M3 (24.6, 15.9, and 5.1, respectively, p < 0.001, repeated measurements). The weight percentiles of recipient twins decreased after FLP, that is, from M1 to M2 (53.4% vs. 33.6%, respectively, p < 0.001, least significant difference [LSD] test). However, the weight percentiles of donor twins increased after delivery, that is, from M2 to M3 (13.2% vs. 26.2%, respectively, p < 0.001, LSD test). Moreover, the TWPs decreased after FLP, that is, from M1 to M2 (66.2% vs. 46.8%, respectively, p = 0.002, LSD test) and increased after delivery, that is, from M2 to M3 (46.8% vs. 63.2%, respectively, p = 0.024, LSD test). The IUGR incidences in donor twins were significantly lower after FLP (77.4% vs. 56.6%, respectively, p = 0.019, McNemar test) and further decreased after delivery (56.6% vs. 37.7%, respectively, p = 0.041, McNemar Test); however, no significant difference was observed in recipient twins’ IUGR incidences among M1, M2, and M3. The donor twin had catch- up growth in body weight, height, and head circumference after delivery, and the recipient twin had catch-up growth in only body height after delivery. Conclusions: the BWD decreased after FLP in fetuses with TTTS mainly because of the decreased weight percentiles of recipient twins. Moreover, it further decreased after delivery mainly because of the increased weight percentiles of donor twins. FLP not only decreased placental perfusion but also improved the TTTS prognosis because of reduced BWD and donor twin IUGR incidence.
Effect of Alumina Particles on the Osteogenic Ability of Osteoblasts
Authors: Sharma Ashish Ranjan , Lee Yeon-Hee , Gankhuyag Buyankhishig , Chakraborty Chiranjib , Lee Sang-Soo
Published: July 2022

Biomaterials are used as implants for bone and dental disabilities. However, wear particles from the implants cause osteolysis following total joint arthroplasty (TJA). Ceramic implants are considered safe and elicit a minimal response to cause periprosthetic osteolysis. However, few reports have highlighted the adverse effect of ceramic particles such as alumina (Al2O3) on various cell types. Hence, we aimed to investigate the effect of Al2O3 particles on osteoprogenitors. A comparative treatment of Al2O3, Ti, and UHMWPE particles to osteoprogenitors at a similar concentration of 200 μg/mL showed that only Al2O3 particles were able to suppress the early and late differentiation markers of osteoprogenitors, including collagen synthesis, alkaline phosphatase (ALP) activity and mRNA expression of Runx2, OSX, Col1α, and OCN. Al2O3 particles even induced inflammation and activated the NFkB signaling pathway in osteoprogenitors. Moreover, bone-forming signals such as the WNT/β-catenin signaling pathway were inhibited by the Al2O3 particles. Al2O3 particles were found to induce the mRNA expression of WNT/β-catenin signaling antagonists such as DKK2, WIF, and sFRP1 several times in osteoprogenitors. Taken together, this study highlights a mechanistic view of the effect of Al2O3 particles on osteoprogenitors and suggests therapeutic targets such as NFĸB and WNT signaling pathways for ceramic particle-induced osteolysis.
Remote-Sensing Measurements of Wave Breaking at Two Pacific Northwest Jettied Inlets
Authors: Holman Rob , Moritz Hans Rod , McMillan James
Published: July 2022

Breaking waves constitute one of the main environmental stressors on coastal structures as well as a leading hazard to navigation in nearshore regions. In this paper, we use camera-based methods to measure wave breaking over two jetty systems in the Pacific Northwest; the North jetty of the Columbia River, Washington state, and the two jetties of Coos Bay, Oregon, as well as over three nearby nearshore dredge disposal areas. Data were collected using the “brightest” images and Argus camera technology over a span of 847 days for the Columbia River and 202 days for Coos Bay. Wave breaking over the Columbia north jetty reached 100% for wave heights greater than 3 m and for tides above mid-tide level and was concentrated on the seaward half of the jetty. For Coos Bay, the south jetty saw substantially more breaking than the north one with the worst overtopping occurring mid-jetty and seeming to be associated with sediment transport through the jetty and into the inlet, as well as possibly the navigation channel. Wave breaking at the Coos Bay inlet mouth was enhanced during ebb flow conditions. Argus imagery analysis showed no evidence of enhanced breaking over any of the three dredge material placement sites.
Dynamic of Tunneled Planing Hulls in Waves
Authors: Roshan Fatemeh , Tavakoli Sasan , Mancini Simone , Dashtimanesh Abbas
Published: July 2022

A tunneled planing craft is a high-speed boat with two tunnels over the hull bottom that are designed to improve the vessel’s performance. Hydrodynamic performance of tunneled planing hulls in calm-water is well-known, however, current information on wave conditions is limited. In this study, two different tunneled planing hulls with two degrees of freedom in heave and pitch motions are studied in regular waves by using the computational fluid dynamics (CFD) method based on the Unsteady Reynolds Averaged Navier-Stokes Equations (URANSE) in conjunction with k−ϵ turbulence model. The results demonstrate that tunneled planing hull motions in waves are nonlinear. In addition, it is found that the dynamic responses of heave and pitch motions as well as occurrence portability of the fly-over phenomenon significantly increases as the Froude number grows. Fly-over motions resulted in vertical motions and acceleration up to 5g, high impact pressure, and large induced drag. At a very high planing speed, after flying over the water surface, when the vessel re-enters the water, the resulting hydrodynamic load leads to a second fly-over motion. Since the fly-over is an unwanted movement with adverse effects, these results can provide a better understanding of the fly-over motion that one may consider in future design for improving the planing hull performance.
The Early Terrestrial Fungal Lineage of Conidiobolus—Transition from Saprotroph to Parasitic Lifestyle
Authors: Gryganskyi Andrii P. , Nie Yong , Hajek Ann E. , Hodge Kathie T. , Liu Xiao-Yong , Aadland Kelsey , Voigt Kerstin , Anishchenko Iryna M. , Kutovenko Vira B. , Kava Liudmyla , Vuek Antonina , Vilgalys Rytas , Huang Bo , Stajich Jason E.
Published: July 2022

Fungi of the Conidiobolus group belong to the family Ancylistaceae (Entomophthorales, Entomophthoromycotina, Zoopagomycota) and include over 70 predominantly saprotrophic species in four similar and closely related genera, that were separated phylogenetically recently. Entomopathogenic fungi of the genus Batkoa are very close morphologically to the Conidiobolus species. Their thalli share similar morphology, and they produce ballistic conidia like closely related entomopathogenic Entomophthoraceae. Ballistic conidia are traditionally considered as an efficient tool in the pathogenic process and an important adaptation to the parasitic lifestyle. Our study aims to reconstruct the phylogeny of this fungal group using molecular and genomic data, ancestral lifestyle and morphological features of the conidiobolus-like group and the direction of their evolution. Based on phylogenetic analysis, some species previously in the family Conidiobolaceae are placed in the new families Capillidiaceae and Neoconidiobolaceae, which each include one genus, and the Conidiobolaceae now includes three genera. Intermediate between the conidiobolus-like groups and Entomophthoraceae, species in the distinct Batkoa clade now belong in the family Batkoaceae. Parasitism evolved several times in the Conidiobolus group and Ancestral State Reconstruction suggests that the evolution of ballistic conidia preceded the evolution of the parasitic lifestyle.
Colletotrichum Species Associated with Anthracnose Disease of Watermelon (Citrullus lanatus) in China
Authors: Guo Zhen , Luo Chao-Xi , Wu Hui-Jie , Peng Bin , Kang Bao-Shan , Liu Li-Ming , Zhang Meng , Gu Qin-Sheng
Published: July 2022

Colletotrichum species are important plant pathogens, causing anthracnose in virtually every crop grown throughout the world. However, little is known about the species that infect watermelon. A total of 526 strains were isolated from diseased watermelon samples of eight major watermelon growing provinces in China. Phylogenetic analyses using seven loci (ITS, gadph, chs-1, his3, act, tub2, and gs) coupled with morphology of 146 representative isolates showed that they belonged to 12 known species of Colletotrichum, including C. aenigma, C. chlorophyti, C. fructicola, C. jiangxiense, C. karstii, C. magnum, C. nymphaeae, C. nigrum, C. orbiculare, C. plurivorum, C. sojae, and C. truncatum and three new species, here described as C. citrulli, C. kaifengense, and C. qilinense. Colletotrichum orbiculare was the dominant species. Pathogenicity tests revealed that all isolates of the species described above were pathogenic, with C. magnum and C. kaifengense being the most aggressive to leaves and fruits, respectively. This is the first report of C. aenigma, C. chlorophyti, C. fructicola, C. jiangxiense, C. nymphaeae, C. nigrum, C. plurivorum, and C. sojae on watermelon. These findings shed light on the Colletotrichum spp. involved in watermelon anthracnose and provide useful information for implementing effective control of watermelon anthracnose in China.
Unveiling the Core Effector Proteins of Oil Palm Pathogen Ganoderma boninense via Pan-Secretome Analysis
Authors: Khairi Mohamad Hazwan Fikri , Nor Muhammad Nor Azlan , Bunawan Hamidun , Abdul Murad Abdul Munir , Ramzi Ahmad Bazli
Published: July 2022

Ganoderma boninense is the major causal agent of basal stem rot (BSR) disease in oil palm, causing the progressive rot of the basal part of the stem. Despite its prominence, the key pathogenicity determinants for the aggressive nature of hemibiotrophic infection remain unknown. In this study, genome sequencing and the annotation of G. boninense T10 were carried out using the Illumina sequencing platform, and comparative genome analysis was performed with previously reported G. boninense strains (NJ3 and G3). The pan-secretome of G. boninense was constructed and comprised 937 core orthogroups, 243 accessory orthogroups, and 84 strain-specific orthogroups. In total, 320 core orthogroups were enriched with candidate effector proteins (CEPs) that could be classified as carbohydrate-active enzymes, hydrolases, and non-catalytic proteins. Differential expression analysis revealed an upregulation of five CEP genes that was linked to the suppression of PTI signaling cascade, while the downregulation of four CEP genes was linked to the inhibition of PTI by preventing host defense elicitation. Genome architecture analysis revealed the one-speed architecture of the G. boninense genome and the lack of preferential association of CEP genes to transposable elements. The findings obtained from this study aid in the characterization of pathogenicity determinants and molecular biomarkers of BSR disease.
SARS-CoV-2 Infection (COVID-19) and Rhinologic Manifestation: Narrative Review
Authors: Lee Seung Hoon , Seo Min Young
Published: July 2022

Patients with severe pneumonia of unknown etiology presented in December 2019 in Wuhan, China. A novel coronavirus, severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), was isolated from the respiratory tracts of these patients. The World Health Organization (WHO) defined respiratory diseases due to SARS-CoV-2 infection as coronavirus disease 2019 (COVID-19). Many researchers have reported that the nasal cavity is an important initial route for SARS-CoV-2 infection and that the spike protein of this virus binds to angiotensin-converting enzyme 2 (ACE2) on epithelial cell surfaces. Therefore, COVID-19 is thought to significantly affect nasal symptoms and various rhinological diseases. In this review, we summarize the association between COVID-19 and various rhinological diseases, such as olfactory dysfunction, rhinosinusitis, and allergic rhinitis.
Contribution of Hypoalbuminemia and Anemia to the Prognostic Value of Plasma p-Cresyl Sulfate and p-Cresyl Glucuronide for Cardiovascular Outcome in Chronic Kidney Disease
Authors: Verbeke Francis , Vanholder Raymond , Van Biesen Wim , Glorieux Griet
Published: July 2022

Free plasma concentrations of protein-bound uremic toxins (PBUTs) may be influenced by serum albumin and hemoglobin. The potential association of serum albumin and hemoglobin with free levels of p-cresyl sulfate (pCS) and p-cresyl glucuronide (pCG) and their predictive value for cardiovascular morbidity and mortality were explored. A total of 523 non-dialysis chronic kidney disease (CKD) stages G1–G5 patients were prospectively followed for the occurrence of fatal or non-fatal cardiovascular events over a 5.5-year period. A negative correlation was found between albumin and between hemoglobin, and both total and free pCS and pCG. In multiple linear regression, PBUTs were negatively associated with eGFR (estimated glomerular filtration rate) and hemoglobin but not albumin. In multivariate Cox regression analysis, albumin was a predictor of outcome, independent of pCS and pCG, without interactions between albumin and pCS or pCG. The relation of low hemoglobin with adverse outcome was lost when albumin was entered into the model. Lower concentrations of pCS and pCG are associated with higher serum albumin and hemoglobin. This may indicate that there are two pathways in the blood that potentially contribute to attenuating the vasculotoxic effects of these PBUTs. The association of PBUTs with cardiovascular risk is not explained by albumin levels, which remains a strong and independent predictor for adverse outcome.
Exploring the Relationship between the Clustering Degree of Children’s Business Formats and the Attractiveness of Commercial Centers in Wuhan by Modifying the Classic Retail Model
Authors: Shan Zhuoran , Shen Xuehan , Yuan Man
Published: July 2022

In recent years, the continued popularity of children’s consumption has made it a new factor that affects the attractiveness of Wuhan’s commercial centers. This study analyzes the characteristics of children’s business format agglomeration in Wuhan commercial centers based on the results of an on-the-spot investigation and estimates the passenger attraction of 66 commercial centers in the main urban area with the support of LBS data. In addition, we set up a control experiment group of commercial centers of various levels and explore the influence mechanism of the density of various types of children’s stores on the attraction of commercial centers by improving the classic retail model. The results indicate the following. (1) Children’s business formats in Wuhan’s commercial centers are active as a whole, and different types of children’s businesses have an unbalanced layout at the different levels of business centers. (2) There are both level ladder and internal level differences in the attractiveness of Wuhan commercial centers. (3) The direction and intensity of the influence of children’s business types on commercial centers of different levels differ. In city-level commercial centers, children’s education and entertainment formats play a role in promotion. In county-level commercial centers, the children’s education format is the most important, and overinvestment in the children’s department store format may not meet expectations. In community-level commercial centers, investment in children’s department stores yielded the best results. (4) Traffic impedance has a stable inhibitory effect at all levels of Wuhan commercial centers, which is in line with the classic retail gravity theory. Further, based on the above results, this paper puts forward suggestions on several types of adaptations that can be applied to children’s consumption stores at different levels of commercial centers to provide support for rationally utilizing the potential of the children’s consumption market.
Regional and Phased Vegetation Responses to Climate Change Are Different in Southwest China
Authors: Wang Meng , An Zhengfeng
Published: July 2022

Southwestern China (SW) is simultaneously affected by the East Asian monsoon, South Asian monsoon and westerly winds, forming a complex and diverse distribution pattern of climate types, resulting in a low interpretation rate of vegetation changes by climate factors in the region. This study explored the response characteristics of vegetation to climatic factors in the whole SW and the core area of typical climate type and the phased changes in response, adopting the form of “top-down”, using linear trend method, moving average method and correlation coefficient, and based on the climate data of CRU TS v. 4.02 for the period 1982–2017 and the annual maximum, 3/4 quantile, median, 1/4 quantile, minimum and average (abbreviated as P100, P75, P50, P25, P5 and Mean) of GIMMS NDVI, which were to characterize vegetation growth conditions. Coupling with the trend and variability of climate change, we identified four major types of climate change in the SW, including the significant increase in both temperature and precipitation (T+*-P+*), the only significant increase in temperature and decrease (T+*-P−) or increase (T+*-P+) of precipitation and no significant change (NSC). We then screened out nine typical areas of climate change types (i.e., core areas (CAs)), followed by one T+*-P+* area, which was located in the center of the lake basin of the Qiangtang Plateau. The response of vegetation to climatic factors in T+*-P+* area/T+*-P+ areas and T+*-P− areas/NSC areas were mainly manifested in an increase and a significant decrease, which makes the response characteristics of vegetation to climatic factors in the whole SW have different directionality at different growth stages. Our results may provide new ideas for clearly showing the complexity and heterogeneity of the vegetation response to climate change in the region under the background of global warming.
Impact of the Cultivated-Land-Management Scale on Fertilizer Reduction—Empirical Evidence from the Countryside of China
Authors: Song Wenhao , Ye Chunhui
Published: July 2022

Reducing the application of chemical fertilizers can improve the quality and promote the sustainable use of cultivated land, which is part of the green and sustainable development of agriculture. Based on the 2019 China Rural Household Panel Survey data, this article evaluated the impact of cultivated-land-management scale on fertilizer reduction via the OLS regression method. The results showed that, after controlling for other factors, the scale of cultivated land management is a strong factor affecting the intensity of fertilizer application by Chinese farmers. According to the results of quantitative analysis, the amount of fertilizer applied per hectare decreased by 20.6% for every 1 ha increase in the average grain sown area per household. The study also found that employing workers in agricultural production and operation can help achieve the aim of fertilizer reduction, but the fact that the head of the household is male, the increase in total household income and the increase in the proportion of household agricultural labor are not conducive to fertilizer reduction. A mechanism-of-action test showed that the expansion of the scale of cultivated land management can drive the reduction of chemical-fertilizer use by urging farmers to adopt agricultural technology and mechanized fertilization. Extensive research found that the fragmentation of cultivated land increased the amount of fertilizer applied per hectare by farmers. Therefore, the fragmentation of cultivated fields is a barrier to decreasing fertilizer application.
The Delivery of Restorative Justice in Youth Offending Teams in England and Wales: Examining Disparities and Highlighting Best Practice
Authors: Banwell-Moore Rebecca
Published: July 2022

Since the establishment of Youth Offending Teams (YOTs) in England and Wales in 1999, all victims of youth crime, must, in accordance with national instruments, be consulted by YOTs as to their wishes and provided with the opportunity to get involved in a restorative justice (RJ) initiative. RJ should be the underlying principle for all youth justice disposals and victims must be invited to be part of the process. If, as evidenced and consistently outlined in guidance, policy and research, the fundamental principle of inclusivity and victim participation are imperative to RJ, then to what extent are YOTs in England and Wales ‘fully’ restorative? Drawing upon the findings of a larger empirical study, this article specifically examines the use of RJ in seven YOTs in England and Wales to demonstrate that RJ has not been fully integrated into practice nor widely embedded into YOT culture. Victims of youth crime, continue to be systematically excluded from RJ. This paper outlines the disparities in the delivery of RJ amongst YOTs, demonstrates the reasons for service delivery disparities and concludes by evidencing best practice.
Molecular Pathways in Clonal Hematopoiesis: From the Acquisition of Somatic Mutations to Transformation into Hematologic Neoplasm
Authors: Gaulin Charles , Kelemen Katalin , Arana Yi Cecilia
Published: July 2022

Hematopoietic stem cell aging, through the acquisition of somatic mutations, gives rise to clonal hematopoiesis (CH). While a high prevalence of CH has been described in otherwise healthy older adults, CH confers an increased risk of both hematologic and non-hematologic diseases. Classification of CH into clonal hematopoiesis of indeterminate potential (CHIP) and clonal cytopenia of undetermined significance (CCUS) further describes this neoplastic myeloid precursor state and stratifies individuals at risk of developing clinically significant complications. The sequential acquisition of driver mutations, such as DNMT3A, TET2, and ASXL1, provide a selective advantage and lead to clonal expansion. Inflammation, microbiome signatures, and external selective pressures also contribute to clonal evolution. Despite significant progress in recent years, the precise molecular mechanisms driving CH transformation to hematologic neoplasms are not well defined. Further understanding of these complex mechanisms may improve risk stratification and introduce therapeutic interventions in CH. Here we discuss the genetic drivers underpinning CH, mechanisms for clonal evolution, and transformation to hematologic neoplasm.
New Potential Immune Biomarkers in the Era of Precision Medicine: Lights and Shadows in Colorectal Cancer
Authors: Damato Angela , Rotolo Martina , Caputo Francesco , Borghi Eleonora , Iachetta Francesco , Pinto Carmine
Published: July 2022

Genetic alterations in CRC have shown a negative predictive and prognostic role in specific target therapies. The onset of immunotherapy has also undergone remarkable therapeutic innovation, although limited to a small subgroup of patients, the MSI-H/dMMR, which represents only 5% of CRC. Research is moving forward to identify whether other biomarkers can predict response to ICIs, despite various limitations regarding expression and identification methods. For this purpose, TMB, LAG3, and PD-L1 expression have been retrospectively evaluated in several solid tumors establishing the rationale to design clinical trials with concurrent inhibition of LAG3 and PD-1 results in a significant advantage in PFS and OS in advanced melanoma patients. Based on these data, there are clinical trials ongoing in the CRC as well. This review aims to highlight what is already known about genetic mutations and genomic alterations in CRC, their inhibition with targeted therapies and immune checkpoints inhibitors, and new findings useful to future treatment strategies.