bluuebunny/crossref_metadata_2025 huggingface dataset:
Red Light-Emitting Water-Soluble Luminescent Iridium-Containing Polynorbornenes: Synthesis, Characterization and Oxygen Sensing Properties in Biological Tissues In Vivo
Authors: Bochkarev Leonid N. , Parshina Yulia P. , Gracheva Yana V. , Kovylina Tatyana A. , Lermontova Svetlana A. , Klapshina Larisa G. , Konev Aleksey N. , Lopatin Mikhail A. , Lukina Maria M. , Komarova Anastasia D. , Shcheslavskiy Vladislav I. , Shirmanova Marina V.
Published: October 2021

New water-soluble polynorbornenes P1–P4 containing oligoether, amino acid groups and luminophoric complexes of iridium(III) were synthesized by ring-opening metathesis polymerization. The polymeric products in organic solvents and in water demonstrate intense photoluminescence in the red spectral region. The polymers P1 and P3 with 1-phenylisoquinoline cyclometalating ligands in iridium fragments reveal 4–6 fold higher emission quantum yields in solutions than those of P2 and P4 that contain iridium complexes with 1-(thien-2-yl)isoquinoline cyclometalating ligands. The emission parameters of P1–P4 in degassed solutions essentially differ from those in the aerated solutions showing oxygen-dependent quenching of phosphorescence. Biological testing of P1 and P3 demonstrates that the polymers do not penetrate into live cultured cancer cells and normal skin fibroblasts and do not possess cytotoxicity within the concentrations and time ranges reasonable for biological studies. In vivo, the polymers display longer phosphorescence lifetimes in mouse tumors than in muscle, as measured using phosphorescence lifetime imaging (PLIM), which correlates with tumor hypoxia. Therefore, preliminary evaluation of the synthesized polymers shows their suitability for noninvasive in vivo assessments of oxygen levels in biological tissues.
Rational Design 2-Hydroxypropylphosphonium Salts as Cancer Cell Mitochondria-Targeted Vectors: Synthesis, Structure, and Biological Properties
Authors: Mironov Vladimir F. , Nemtarev Andrey V. , Tsepaeva Olga V. , Dimukhametov Mudaris N. , Litvinov Igor A. , Voloshina Alexandra D. , Pashirova Tatiana N. , Titov Eugenii A. , Lyubina Anna P. , Amerhanova Syumbelya K. , Gubaidullin Aidar T. , Islamov Daut R.
Published: October 2021

It has been shown for a wide range of epoxy compounds that their interaction with triphenylphosphonium triflate occurs with a high chemoselectivity and leads to the formation of (2-hydroxypropyl)triphenylphosphonium triflates 3 substituted in the 3-position with an alkoxy, alkylcarboxyl group, or halogen, which were isolated in a high yield. Using the methodology for the disclosure of epichlorohydrin with alcohols in the presence of boron trifluoride etherate, followed by the substitution of iodine for chlorine and treatment with triphenylphosphine, 2-hydroxypropyltriphenylphosphonium iodides 4 were also obtained. The molecular and supramolecular structure of the obtained phosphonium salts was established, and their high antitumor activity was revealed in relation to duodenal adenocarcinoma. The formation of liposomal systems based on phosphonium salt 3 and L-α-phosphatidylcholine (PC) was employed for improving the bioavailability and reducing the toxicity. They were produced by the thin film rehydration method and exhibited cytotoxic properties. This rational design of phosphonium salts 3 and 4 has promising potential of new vectors for targeted delivery into mitochondria of tumor cells.
The Antiarrhythmic Activity of Novel Pyrrolidin-2-one Derivative S-75 in Adrenaline-Induced Arrhythmia
Authors: Lustyk Klaudia , Sałaciak Kinga , Zaręba Paula , Siwek Agata , Sapa Jacek , Pytka Karolina
Published: October 2021

Arrhythmia is a quivering or irregular heartbeat that can often lead to blood clots, stroke, heart failure, and other heart-related complications. The limited efficacy and safety of antiarrhythmic drugs require the design of new compounds. Previous research indicated that pyrrolidin-2-one derivatives possess an affinity for α1-adrenergic receptors. The blockade of α1-adrenoceptor may play a role in restoring normal sinus rhythm; therefore, we aimed to verify the antiarrhythmic activity of novel pyrrolidin-2-one derivative S-75. In this study, we assessed the influence on sodium, calcium, potassium channels, and β1-adrenergic receptors to investigate the mechanism of action of S-75. Lack of affinity for β1-adrenoceptors and weak effects on ion channels decreased the role of these adrenoceptors and channels in the pharmacological activity of S-75. Next, we evaluated the influence of S-75 on normal ECG in rats and isolated rat hearts, and the tested derivative did not prolong the QTc interval, which may confirm the lack of the proarrhythmic potential. We tested antiarrhythmic activity in adrenaline-, aconitine- and calcium chloride-induced arrhythmia models in rats. The studied compound showed prophylactic antiarrhythmic activity in the adrenaline-induced arrhythmia, but no significant activity in the model of aconitine- or calcium chloride-induced arrhythmia. In addition, S-75 was not active in the model of post-reperfusion arrhythmias of the isolated rat hearts. Conversely, the compound showed therapeutic antiarrhythmic properties in adrenaline-induced arrhythmia, reducing post-arrhythmogen heart rhythm disorders, and decreasing animal mortality. Thus, we suggest that the blockade of α1-adrenoceptor might be beneficial in restoring normal heart rhythm in adrenaline-induced arrhythmia.
Towards a Better Understanding of Verapamil Release from Kollicoat SR:IR Coated Pellets Using Non-Invasive Analytical Tools
Authors: Fahier Julie , Vukosavljevic Branko , De Kinder Laure , Florin Hugues , Goossens Jean-François , Windbergs Maike , Siepmann Florence , Siepmann Juergen , Muschert Susanne
Published: October 2021

The aim of this study was to gain deeper insight into the mass transport mechanisms controlling drug release from polymer-coated pellets using non-invasive analytical tools. Pellet starter cores loaded with verapamil HCl (10% loading, 45% lactose, 45% microcrystalline cellulose) were prepared by extrusion/spheronization and coated with 5% Kollicoat SR:IR 95:5 or 10% Kollicoat SR:IR 90:10. Drug release was measured from ensembles of pellets as well as from single pellets upon exposure to acetate buffer pH = 3.5 and phosphate buffer pH = 7.4. The swelling of single pellets was observed by optical microscopy, while dynamic changes in the pH in the pellet cores were monitored by fluorescence spectroscopy. Also, mathematical modeling using a mechanistically realistic theory as well as SEM and Raman imaging were applied to elucidate whether drug release mainly occurs by diffusion through the intact film coatings or whether crack formation in the film coatings plays a role. Interestingly, fluorescence spectroscopy revealed that the pH within the pellet cores substantially differed upon exposure to acetate buffer pH = 3.5 and phosphate buffer pH = 7.4, resulting in significant differences in drug solubility (verapamil being a weak base) and faster drug release at lower pH: from ensembles of pellets and single pellets. The monitoring of drug release from and the swelling of single pellets indicated that crack formation in the film coatings likely plays a major role, irrespective of the Kollicoat SR:IR ratio/coating level. This was confirmed by mathematical modeling, SEM and Raman imaging. Importantly, the latter technique allowed also for non-invasive measurements, reducing the risk of artifact creation associated with sample cutting with a scalpel.
Optimization and Development of Selective Histone Deacetylase Inhibitor (MPT0B291)-Loaded Albumin Nanoparticles for Anticancer Therapy
Authors: Putri Athika Darumas , Chen Pai-Shan , Su Yu-Lin , Lin Jia-Pei , Liou Jing-Ping , Hsieh Chien-Ming
Published: October 2021

Histone deacetylase (HDAC) inhibitors have emerged as a new class of antitumor agent for various types of tumors. MPT0B291, a novel selective inhibitor of HDAC6, demonstrated significant antiproliferative activity in various human cancer cell types. However, MPT0B291 has very low water solubility, which limits its clinical use for cancer therapy. In the current study, MPT0B291 was encapsulated in human serum albumin (HSA), and its anticancer activities were investigated. Nanoparticles (NPs) were prepared using two-stage emulsification resulting in 100~200-nm NPs with a fine size distribution (polydispersity index of <0.3). The in vitro drug release profiles of MPT0B291-loaded HSA NPs presented sustained-release properties. The cytotoxic effect on MIA PaCa-2 human pancreatic carcinoma cells was found to be similar to MPT0B291-loaded HSA NPs and the free-drug group. The albumin-based formulation provided a higher maximum tolerated dose than that of a drug solution with reduced toxicity toward normal cells. Furthermore, in vivo pharmacokinetic studies demonstrated an effective increase (5~8-fold) in the bioavailability of NPs containing MPT0B291 loaded in HSA compared to the free-drug solution with an extended circulation time (t1/2) leading to significantly enhanced efficacy of anticancer treatment.
Quantification of Fluid Volume and Distribution in the Paediatric Colon via Magnetic Resonance Imaging
Authors: Goelen Jan , Alexander Benoni , Wijesinghe Haren Eranga , Evans Emily , Pawar Gopal , Horniblow Richard D. , Batchelor Hannah K.
Published: October 2021

Previous studies have used magnetic resonance imaging (MRI) to quantify the fluid in the stomach and small intestine of children, and the stomach, small intestine and colon of adults. This is the first study to quantify fluid volumes and distribution using MRI in the paediatric colon. MRI datasets from 28 fasted (aged 0–15 years) and 18 fluid-fed (aged 10–16 years) paediatric participants were acquired during routine clinical care. A series of 2D- and 3D-based software protocols were used to measure colonic fluid volume and localisation. The paediatric colon contained a mean volume of 22.5 mL ± 41.3 mL fluid, (range 0–167.5 mL, median volume 0.80 mL) in 15.5 ± 17.5 discreet fluid pockets (median 12). The proportion of the fluid pockets larger than 1 mL was 9.6%, which contributed to 94.5% of the total fluid volume observed. No correlation was detected between all-ages and colonic fluid volume, nor was a difference in colonic fluid volumes observed based on sex, fed state or age group based on ICH-classifications. This study quantified fluid volumes within the paediatric colon, and these data will aid and accelerate the development of biorelevant tools to progress paediatric drug development for colon-targeting formulations.
Pleiotropic Effects of Atorvastatin Result in a Downregulation of the Carboxypeptidase U System (CPU, TAFIa, CPB2) in a Mouse Model of Advanced Atherosclerosis
Authors: Claesen Karen , Roth Lynn , Mertens Joachim C. , Hermans Karlijn , Sim Yani , Hendriks Dirk
Published: October 2021

Statins (hydroxymethyl-glutaryl-CoA-reductase inhibitors) lower procarboxypeptidase U (proCPU, TAFI, proCPB2). However, it is challenging to prove whether this is a lipid or non-lipid-related pleiotropic effect, since statin treatment decreases cholesterol levels in humans. In apolipoprotein E-deficient mice with a heterozygous mutation in the fibrillin-1 gene (ApoE−/−Fbn1C1039G+/−), a model of advanced atherosclerosis, statins do not lower cholesterol. Consequently, studying cholesterol-independent effects of statins can be achieved more straightforwardly in these mice. Female ApoE −/−Fbn1C1039G+/− mice were fed a Western diet (WD). At week 10 of WD, mice were divided into a WD group (receiving WD only) and a WD + atorvastatin group (receiving 10 mg/kg/day atorvastatin +WD) group. After 15 weeks, blood was collected from the retro-orbital plexus, and the mice were sacrificed. Total plasma cholesterol and C-reactive protein (CRP) were measured with commercially available kits. Plasma proCPU levels were determined with an activity-based assay. Total plasma cholesterol levels were not significantly different between both groups, while proCPU levels were significantly lower in the WD + atorvastatin group. Interestingly proCPU levels correlated with CRP and circulating monocytes. In conclusion, our results confirm that atorvastatin downregulates proCPU levels in ApoE−/−Fbn1C1039G+/− mice on a WD, and evidence was provided that this downregulation is a pleiotropic effect of atorvastatin treatment.
Biogenic ZnO Nanoparticles Synthesized from Origanum vulgare Abrogates Quorum Sensing and Biofilm Formation in Opportunistic Pathogen Chromobacterium violaceum
Authors: Kamli Majid Rasool , Malik Maqsood Ahmad , Srivastava Vartika , Sabir Jamal S. M. , Mattar Ehab H. , Ahmad Aijaz
Published: October 2021

This study presents an inexpensive, eco-friendly, and simple green synthesis of ZnO nanoparticles using Origanum vulgare extract. These nanoparticles are non-hazardous, environmentally friendly, and cheaper than other methods of biosynthesis. Ongoing research determines the role of phytochemicals in the fabrication and biosynthesis of ZnO NPs and their role in antibacterial activity and biomedical applications. Characterizations by fourier transform infrared spectroscopy (FTIR), diffuse reflectance UV-visible spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) determine the successful biosynthesis of ZnO NPs. Meanwhile, TEM and X-ray diffraction studies approximated the spherical morphology and crystalline nature of biosynthesized ZnO NPs of nano size in the range of 20–30 nm. The global increase in drug resistance necessitates the search for new drugs with different mechanisms of action. Quorum sensing (QS), a cell-to-cell communication, has gained attention as an emerging drug target. It controls numerous biochemical processes in bacteria, which are essential for their survival and pathogenicity. The potential of nanomedicines has also been tested to synthesize new antibiotics to tackle drug resistance. ZnO NPs were explored for their antibacterial, antiquorum sensing, and antibiofilm activities with a bioreporter strain of Chromobacterium violaceum. Susceptibility testing results indicated the potential antibacterial activity of ZnO NPs with a minimum inhibitory concentration (MIC) of 4 µg/mL and minimum bactericidal concentration (MBC) of 16 µg/mL. Antiquorum-sensing assays revealed that these nanoparticles inhibit quorum sensing with minimum antiquorum sensing activity (MQSIC) of 1 µg/mL, without causing any bacterial growth inhibition. In addition, ZnO NPs inhibit biofilm formation at inhibitory and higher concentrations. RT-qPCR results supported the downregulation of the quorum sensing genes when C. violaceum was treated with ZnO NPs. The outcomes of this study are promising with regard to the biofilm and quorum sensing, emphasizing the potential applications of ZnO NPs against bacterial communication and biofilm formation.
Quantitative N-Glycan Profiling of Therapeutic Monoclonal Antibodies Performed by Middle-Up Level HILIC-HRMS Analysis
Authors: Duivelshof Bastiaan L. , Denorme Steffy , Sandra Koen , Liu Xiaoxiao , Beck Alain , Lauber Matthew A. , Guillarme Davy , D’Atri Valentina
Published: October 2021

The identification and accurate quantitation of the various glycoforms contained in therapeutic monoclonal antibodies (mAbs) is one of the main analytical needs in the biopharmaceutical industry, and glycosylation represents a crucial critical quality attribute (CQA) that needs to be addressed. Currently, the reference method for performing such identification/quantitation consists of the release of the N-glycan moieties from the mAb, their labelling with a specific dye (e.g., 2-AB or RFMS) and their analysis by HILIC-FLD or HILIC-MS. In this contribution, the potential of a new cost- and time-effective analytical approach performed at the protein subunit level (middle-up) was investigated for quantitative purposes and compared with the reference methods. The robustness of the approach was first demonstrated by performing the relative quantification of the glycoforms related to a well characterized mAb, namely adalimumab. Then, the workflow was applied to various glyco-engineered mAb products (i.e., obinutuzumab, benralizumab and atezolizumab). Finally, the glycosylation pattern of infliximab (Remicade®) was assessed and compared to two of its commercially available biosimilars (Remsima® and Inflectra®). The middle-up analysis proved to provide accurate quantitation results and has the added potential to be used as multi-attribute monitoring method.
Activators and Inhibitors of Protein Kinase C (PKC): Their Applications in Clinical Trials
Authors: Kawano Takahito , Inokuchi Junichi , Eto Masatoshi , Murata Masaharu , Kang Jeong-Hun
Published: October 2021

Protein kinase C (PKC), a family of phospholipid-dependent serine/threonine kinase, is classed into three subfamilies based on their structural and activation characteristics: conventional or classic PKC isozymes (cPKCs; α, βI, βII, and γ), novel or non-classic PKC isozymes (nPKCs; δ, ε, η, and θ), and atypical PKC isozymes (aPKCs; ζ, ι, and λ). PKC inhibitors and activators are used to understand PKC-mediated intracellular signaling pathways and for the diagnosis and treatment of various PKC-associated diseases, such as cancers, neurological diseases, cardiovascular diseases, and infections. Many clinical trials of PKC inhibitors in cancers showed no significant clinical benefits, meaning that there is a limitation to design a cancer therapeutic strategy targeting PKC alone. This review will focus on the activators and inhibitors of PKC and their applications in clinical trials.
Regenerated Cellulose Products for Agricultural and Their Potential: A Review
Authors: Zainul Armir Nur Amira , Zulkifli Amalia , Gunaseelan Shamini , Palanivelu Swarna Devi , Salleh Kushairi Mohd , Che Othman Muhamad Hafiz , Zakaria Sarani
Published: October 2021

Cellulose is one of the most abundant natural polymers with excellent biocompatibility, non-toxicity, flexibility, and renewable source. Regenerated cellulose (RC) products result from the dissolution-regeneration process risen from solvent and anti-solvent reagents, respectively. The regeneration process changes the cellulose chain conformation from cellulose I to cellulose II, leads the structure to have more amorphous regions with improved crystallinity, and inclines towards extensive modification on the RC products such as hydrogel, aerogel, cryogel, xerogel, fibers, membrane, and thin film. Recently, RC products are accentuated to be used in the agriculture field to develop future sustainable agriculture as alternatives to conventional agriculture systems. However, different solvent types and production techniques have great influences on the end properties of RC products. Besides, the fabrication of RC products from solely RC lacks excellent mechanical characteristics. Thus, the flexibility of RC has allowed it to be homogenously blended with other materials to enhance the final products’ properties. This review will summarize the properties and preparation of potential RC-based products that reflect its application to replace soil the plantation medium, govern the release of the fertilizer, provide protection on crops and act as biosensors.
Effect of Multiple Enamel Surface Treatments on Micro-Shear Bond Strength
Authors: Daher René , Krejci Ivo , Mekki Mustapha , Marin Charlotte , Di Bella Enrico , Ardu Stefano
Published: October 2021

Objectives: The aim of this study was to evaluate the effect of different enamel surface treatments on the micro-shear bond strength. Materials and methods: Sixty-four approximal surfaces from freshly extracted molars were randomly assigned to eight groups, according to combinations of the following enamel surface treatments: ground or unground, 37.5% phosphoric acid etching time of 15 or 30 s, and with or without primer application. The same bonding agent (Optibond FL™ Adhesive, Kerr) was then used for all groups, and a 1.8 mm diameter resin composite (Harmonize™, Kerr) cylinder was built up on the bonded surface. Samples underwent a shear force test at a crosshead speed of 1 mm/min until failure. Bond strength was calculated, and failure modes were inspected under an optical microscope. Results: Bond-strength values ranged from 8.2 MPa for 15 s etched unground enamel with primer application to 19.6 MPa for 30 s etched ground enamel without primer application. ANOVA and Fisher’s LSD post hoc tests revealed significant differences between the groups. Conclusions: Etching time and grinding have a statistically significant effect on the micro-shear bond strength of a three-step etch-and-rinse adhesive system on enamel. Primer application does not seem to be beneficial for enamel adhesion.
Injection Molding of Low-Density Polyethylene (LDPE) as a Model Polymer: Effect of Molding Parameters on the Microstructure and Crystallinity
Authors: Leyva-Porras César , Balderrama-Aguilar Andrea , Estrada-Ávila Yael , Espelosín-Gómez Iñaki , Mendoza-Duarte Mónica , Piñón-Balderrama Claudia , Saavedra-Leos María Zenaida , Estrada-Moreno Iván
Published: October 2021

Due to its relatively simple structure, low-density polyethylene (LDPE) can be considered as a model polymer for the study of its properties. Herein, the effect of processing variables on the microstructure and crystallinity of injection-molded LDPE specimens was quantitatively determined. The polymer was injected at different temperature conditions in the barrel and the mold. The specimens were characterized by scanning electron microscopy and X-ray diffraction. With the data obtained, an analysis of variance (ANOVA) was carried out, and response surface graphs (SRP) were constructed to quantify and to observe the behavior of the processing variables, respectively. Different models were obtained to predict the effect of the experimental factors on the response variables. The results showed that the interaction of the two temperatures has the greatest effect on the size of the spherulite, while the temperature of the mold affects the crystallinity. The SRP showed different behaviors: for the spherulite, the size increases with the mold temperature, while for the crystallinity, higher values were observed at an intermediate mold temperature and a low melt temperature. The results presented herein are valuable for setting empirical relations between the microstructure, crystallinity, and the molding conditions of LDPE.
Cellulosic/Polyvinyl Alcohol Composite Hydrogel: Synthesis, Characterization and Applications in Tissue Engineering
Authors: Stricher Mathilde , Sarde Claude-Olivier , Guénin Erwann , Egles Christophe , Delbecq Frédéric
Published: October 2021

The biomedical field still requires composite materials for medical devices and tissue engineering model design. As part of the pursuit of non-animal and non-proteic scaffolds, we propose here a cellulose-based material. In this study, 9%, 18% and 36% dialdehyde-functionalized microcrystalline celluloses (DAC) were synthesized by sodium periodate oxidation. The latter was subsequently coupled to PVA at ratios 1:2, 1:1 and 2:1 by dissolving in N-methyl pyrrolidone and lithium chloride. Moulding and successive rehydration in ethanol and water baths formed soft hydrogels. While oxidation effectiveness was confirmed by dialdehyde content determination for all DAC, we observed increasing hydrolysis associated with particle fragmentation. Imaging, FTIR and XDR analysis highlighted an intertwined DAC/PVA network mainly supported by electrostatic interactions, hemiacetal and acetal linkage. To meet tissue engineering requirements, an interconnected porosity was optimized using 0–50 µm salts. While the role of DAC in strengthening the hydrogel was identified, the oxidation ratio of DAC showed no distinct trend. DAC 9% material exhibited the highest indirect and direct cytocompatibility creating spheroid-like structures. DAC/PVA hydrogels showed physical stability and acceptability in vivo that led us to propose our DAC 9%/PVA based material for soft tissue graft application.
Pre-Heating Effect on Monomer Elution and Degree of Conversion of Contemporary and Thermoviscous Bulk-Fill Resin-Based Dental Composites
Authors: Kincses Dóra , Böddi Katalin , Őri Zsuzsanna , Lovász Bálint Viktor , Jeges Sára , Szalma József , Kunsági-Máté Sándor , Lempel Edina
Published: October 2021

Detection of unreacted monomers from pre-heated resin-based dental composites (RBC) is not a well-investigated topic so far. The objectives were to determine the temperature changes during the application and polymerization, the degree of conversion (DC) and unreacted monomer elution of room temperature (RT), and pre-heated thermoviscous [VisCalor Bulk(VCB)] and high-viscosity full-body contemporary [Filtek One Bulk(FOB)] bulk-fill RBCs. The RBCs’ temperatures during the sample preparation were recorded with a K-type thermocouple. The DC at the top and bottom was measured with micro-Raman spectroscopy and the amounts of eluted BisGMA, UDMA, DDMA, and TEGDMA were assessed with High-Performance Liquid Chromatography. The temperatures of the pre-heated RBCs decreased rapidly during the manipulation phase. The temperature rise during photopolymerization reflects the bottom DCs. The differences in DC% between the top and the bottom were significant. RT VCB had a lower DC% compared to FOB. Pre-heating did not influence the DC, except on the bottom surface of FOB where a significant decrease was measured. Pre-heating significantly decreased the elution of BisGMA, UDMA, DDMA in the case of FOB, meanwhile, it had no effect on monomer release from VCB, except TEGDMA, which elution was decreased. In comparison, RBC composition had a stronger influence on DC and monomer elution, than pre-cure temperature.
Quasi-Static Compression Properties of Bamboo and PVC Tube Reinforced Polymer Foam Structures
Authors: Amelia J. J. N. , Zuhri M. Y. M. , Leman Z. , Zahari N. I. , As’arry A. , Ilyas R. A.
Published: October 2021

In recent years, there has been a growing interest for composite materials due to the superior capability to absorb energy and lightweight factor. These properties are compatible to be utilized in the development for transportation system as it can reduce the fuel consumption and also minimize the effect of crash to the passenger. Therefore, the aim for this project is to study the compression strength and energy absorbing capability for Polyvinyl chloride (PVC) and bamboo tubes reinforced with foam. Several parameters are being considered, these being the effect of single and multiple tube reinforced foam structure, foam density, diameter of the tube as well as effect of different crosshead speed. The results showed that increasing the relative foam density will led to an increase in the compression strength and specific energy absorption (SEA) values. Furthermore, a significant increase of compression strength can be seen when several tubes are introduced into the foam while SEA remained almost the same. Finally, the influence of crosshead below 20 mm/min did not vary significantly for both compression strength and SEA.
Disposable Food Packaging and Serving Materials—Trends and Biodegradability
Authors: Dybka-Stępień Katarzyna , Antolak Hubert , Kmiotek Magdalena , Piechota Dominik , Koziróg Anna
Published: October 2021

Food is an integral part of everyone’s life. Disposable food serving utensils and tableware are a very convenient solution, especially when the possibility of the use of traditional dishes and cutlery is limited (e.g., takeaway meals). As a result, a whole range of products is available on the market: plates, trays, spoons, forks, knives, cups, straws, and more. Both the form of the product (adapted to the distribution and sales system) as well as its ecological aspect (biodegradability and life cycle) should be of interest to producers and consumers, especially considering the clearly growing trend of “eco-awareness”. This is particularly important in the case of single-use products. The aim of the study was to present the current trends regarding disposable utensils intended for contact with food in the context of their biodegradability. This paper has summarized not only conventional polymers but also their modern alternatives gaining the attention of manufacturers and consumers of single-use products (SUPs).
Recycling of Residual Polymers Reinforced with Natural Fibers as a Sustainable Alternative: A Review
Authors: Fuentes Molina Natalia , Fragozo Brito Yoleimis , Polo Benavides Jesús Manuel
Published: October 2021

The latest advances in green alternatives are being addressed with bio-based solutions, with uses and applications in new areas due to their wide potential, low cost, lightness, renewability, biodegradability, impact toughness, fatigue resistance, and other specific properties. Natural fibers are sustainable materials that have led researchers to test their viability as alternative reinforcements in residual polymers to meet required engineering specifications; therefore, it is essential to continue making progress in replacing conventional materials. This review is expected to provide an overview of the current scopes and future prospects of biocomposites from polymers reinforced with natural fibers with a focus on the following: i. recycling of residual polymers; ii. available natural fibers and their components in the context of engineering applications; iii. the behavior of the structural modifications of the natural fibers with the physical and chemical treatments in the matrix interaction as reinforcements of the residual polymers; and iv. applications for the development of innovative, efficient, and sustainable solutions for successful, environmentally responsible products.
Meshkwajisewin: Paradigm Shift
Authors: Matthews Maureen Anne , Roulette Roger , Wilson James Brook
Published: October 2021

In 2012, the Manitoba Museum began the development of an exhibit called “We Are All Treaty People”. Mindful of recent scholarship on animacy and the ontological turn in museum ethnography, this paper examines how this exhibit reversed decades of practice regarding ceremonial artefacts. Twelve pipes, formerly removed from view because of their ceremonial status, have now, as celebrated animate entities, become teachers in a collaboratively developed exhibit about treaties. They will work to educate thousands of visitors, many of them Indigenous children who visit the museum annually. The exhibit was imagined, shaped, and made possible by the Elders Council of the Association of Manitoba Chiefs and the Treaty Relations Commission of Manitoba who treat the pipes as active social partners and, from the outset, intended that the pipes would boldly instantiate Indigenous agency in treaty making. The relational world of the pipes has increased exponentially since they have become public actors in the museum, and more importantly, they have formed deep bonds with the school children and Elders of the community of Roseau River First Nation. They go to the school yearly to be celebrated, sung to, feasted, smoked, and honoured and return to the museum restored and ready for their newfound educational and diplomatic work.
Optimizing Sensor Position with Virtual Sensors in Human Activity Recognition System Design
Authors: Xia Chengshuo , Sugiura Yuta
Published: October 2021

Human activity recognition (HAR) systems combined with machine learning normally serve users based on a fixed sensor position interface. Variations in the installing position will alter the performance of the recognition and will require a new training dataset. Therefore, we need to understand the role of sensor position in HAR system design to optimize its effect. In this paper, we designed an optimization scheme with virtual sensor data for the HAR system. The system is able to generate the optimal sensor position from all possible locations under a given sensor number. Utilizing virtual sensor data, the training dataset can be accessed at low cost. The system can help the decision-making process of sensor position selection with great accuracy using feedback, as well as output the classifier at a lower cost than a conventional training model.
Selective Separation and Analysis of Catecholamines in Urine Based on Magnetic Solid Phase Extraction by Mercaptophenylboronic Acid Functionalized Fe3O4-NH2@Au Magnetic Nanoparticles Coupled with HPLC
Authors: Han Qing , Wu Xiaoxiao , Cao Yi , Zhang Hua , Zhao Yuqin , Kang Xuejun , Zhu Huaiyuan
Published: October 2021

A novel magnetic solid phase extraction based on mercaptophenylboronic acid (MPBA)-functionalized Fe3O4-NH2@Au nanomaterial (Fe3O4-NH2@Au-MPBA) was developed for selective separation and enrichment of catecholamines (including dopamine, norepinephrine and adrenaline). Fe3O4-NH2@Au-MPBA nanoparticles were achieved by self-assembly-anchoring MPBA molecules on the surface of Fe3O4-NH2@Au nanocomposites, which were synthesized via a facial ultrasonic auxiliary in situ reduction process. The interaction between cis-diol from catecholamines and boronic acid was reversible and could be flexibly controlled by adjusting pH value. The catecholamines could be quickly adsorbed by Fe3O4-NH2@Au-MPBA in weak alkaline solution (pH 8.0–9.0) and subsequently released in acid solution (pH 1.0–2.0). The process of adsorption and dissociation was very fast. Furthermore, the three catecholamines could be detected in urine from children by high performance liquid chromatography (HPLC) with electrochemical detector. Under optimal conditions, norepinephrine (NE), epinephrine (EP) and dopamine (DA) were separated very well from internal standard and exhibited a good linearity in the range of 2.5–500.0 ng mL−1, with correlation coefficients of r2 > 0.9907. Limits of detection (LOD) (signal to noise = 3) were 0.39, 0.27 and 0.60 ng mL−1 for NE, EP and DA, respectively. Recoveries for the spiked catecholamines were in the range of 85.4–105.2% with the relative standard deviation (RSD) < 11.5%.
Consumption Structure in Urban and Rural Areas and Self-Rated Health of the Elderly: A Survey Based on Chinese General Social Survey
Authors: Liu Zhaojing , Li Bin
Published: October 2021

Health inequality is an aspect of social inequality, and has now become an important problem in the current society. This article uses the data from the Chinese General Social Survey (2017) (CGSS2017) and uses the Multinomial Logistic Models method to analyze the elderly population (over 60 years old) across the country. The study found that the differences in the basic consumption of food and daily necessities among the elderly are relatively small; while consumer goods that reflect the differentiation of social classes such as clothing consumption and cultural consumption have a significant impact on the elderly. Travel consumption status also has a certain impact on the self-rated health choices of the elderly, but housing consumption has no effect on these choices. Thess results pave the way for investigating health from the perspective of socioeconomic status in academic circles. By using this consumption pattern analysis it is possible to analyze the health of the elderly population more effectively. In the future supply of consumer goods, it is possible to strengthen the consumption and supply of cultural tastes for the elderly, enhance the beauty and value of the lifestyle of the elderly, and increase the health of the elderly. At the same time, due to the significant impact of urban–rural differences in the health of the elderly, it is necessary to improve the living security level of the rural elderly, reduce the difference in public services between urban and rural elderly groups, and promote urban–rural integration.
Cloud-Based Booking Platforms in Warehouse Operations
Authors: Giuffrida Maria , Mangiaracina Riccardo , Burki Umar
Published: October 2021

This study evaluates the implementation costs of cloud-based booking platforms (CBBPs) to manage vehicle queues in warehouses and investigates how cloud-based booking platforms support inter-organizational logistics processes. To understand the impact of CBBPs, we interviewed logistics managers concerning the structure of the CBBP processes, salient platform features, and their effects on inter-organizational dynamics. In addition, we conducted an analytical modeling simulation to compute the economic impact of each investment scenario. Our results show that CBBP benefits, and their financial viability, are sensitive to the size of a company and the selected decision driver (cost vs. time optimization). Hence, our findings challenge the standard view that adopting digital technologies in inter-organizational settings is an easy task.
Evaluation of Cyanobacterial Bloom from Lake Taihu as a Protein Substitute in Fish Diet—A Case Study on Tilapia
Authors: Huo Yan , Li Yuanze , Guo Wei , Liu Jin , Yang Cuiping , Li Lin , Liu Haokun , Song Lirong
Published: October 2021

The utility of cyanobacterial bloom is often hindered by concerns about the toxin content. Over three years of investigation, we found that the toxin content of cyanobacterial bloom in Lake Taihu was always low in June and higher in late summer and autumn. The findings enabled us to compare the effects of diets containing low and high toxic cyanobacterial blooms on the growth and consumption safety of tilapia. There were no negative effects on the growth of tilapia, and the muscle seemed to be safe for human consumption in the treatment of 18.5% low toxic cyanobacterial bloom. Therefore, limitations of the utilization of cyanobacterial biomass can be overcome by selecting low toxic cyanobacterial bloom that can be found and collected in large lakes.
Retinaldehyde Dehydrogenase Inhibition-Related Adverse Outcome Pathway: Potential Risk of Retinoic Acid Synthesis Inhibition during Embryogenesis
Authors: Cho Kichul , Lee Sang-Moo , Heo Jina , Kwon Yong Min , Chung Dawoon , Yu Woon-Jong , Bae Seung Seob , Choi Grace , Lee Dae-Sung , Kim Youngjun
Published: October 2021

Retinoic acid (RA) is one of the factors crucial for cell growth, differentiation, and embryogenesis; it interacts with the retinoic acid receptor and retinoic acid X receptor to eventually regulate target gene expression in chordates. RA is transformed from retinaldehyde via oxidization by retinaldehyde dehydrogenase (RALDH), which belongs to the family of oxidoreductases. Several chemicals, including disulphiram, diethylaminobenzaldehyde, and SB-210661, can effectively inhibit RALDH activity, potentially causing reproductive and developmental toxicity. The modes of action can be sequentially explained based on the molecular initiating event toward key events, and finally the adverse outcomes. Adverse outcome pathway (AOP) is a conceptual and theoretical framework that describes the sequential chain of casually liked events at different biological levels from molecular events to adverse effects. In the present review, we discussed a recently registered AOP (AOP297; inhibition of retinaldehyde dehydrogenase leads to population decline) to explain and support the weight of evidence for RALDH inhibition-related developmental toxicity using the existing knowledge.
Comparison of PCR versus PCR-Free DNA Library Preparation for Characterising the Human Faecal Virome
Authors: Hsieh Shen-Yuan , Tariq Mohammad A. , Telatin Andrea , Ansorge Rebecca , Adriaenssens Evelien M. , Savva George M. , Booth Catherine , Wileman Tom , Hoyles Lesley , Carding Simon R.
Published: October 2021

The human intestinal microbiota is abundant in viruses, comprising mainly bacteriophages, occasionally outnumbering bacteria 10:1 and is termed the virome. Due to their high genetic diversity and the lack of suitable tools and reference databases, the virome remains poorly characterised and is often referred to as “viral dark matter”. However, the choice of sequencing platforms, read lengths and library preparation make study design challenging with respect to the virome. Here we have compared the use of PCR and PCR-free methods for sequence-library construction on the Illumina sequencing platform for characterising the human faecal virome. Viral DNA was extracted from faecal samples of three healthy donors and sequenced. Our analysis shows that most variation was reflecting the individually specific faecal virome. However, we observed differences between PCR and PCR-free library preparation that affected the recovery of low-abundance viral genomes. Using three faecal samples in this study, the PCR library preparation samples led to a loss of lower-abundance vOTUs evident in their PCR-free pairs (vOTUs 128, 6202 and 8364) and decreased the alpha-diversity indices (Chao1 p-value = 0.045 and Simpson p-value = 0.044). Thus, differences between PCR and PCR-free methods are important to consider when investigating “rare” members of the gut virome, with these biases likely negligible when investigating moderately and highly abundant viruses.
Analysis of SARS-CoV-2 Genomes from West Java, Indonesia
Authors: Fibriani Azzania , Stephanie Rebecca , Alfiantie Afifah Alifia , Siregar Agust Leo Fany , Pradani Gusti Ayu Prani , Yamahoki Nicholas , Purba William Steflandel , Alamanda Cut Nur Cinthia , Rahmawati Ema , Rachman Rifky Waluyajati , Robiani Rini , Ristandi Ryan Bayusantika
Published: October 2021

West Java Health Laboratory (WJHL) is one of the many institutions in Indonesia that have sequenced SARS-CoV-2 genome. Although having submitted a large number of sequences since September 2020, however, these submitted data lack advanced analyses. Therefore, in this study, we analyze the variant distribution, hotspot mutation, and its impact on protein structure and function of SARS-CoV-2 from the collected samples from WJHL. As many as one hundred sixty-three SARS-CoV-2 genome sequences submitted by West Java Health Laboratory (WJHL), with collection dates between September 2020 and June 2021, were retrieved from GISAID. Subsequently, the frequency and distribution of non-synonymous mutations across different cities and regencies from these samples were analyzed. The effect of the most prevalent mutations from dominant variants on the stability of their corresponding proteins was examined. The samples mostly consisted of people of working-age, and were distributed between female and male equally. All of the sample sequences showed varying levels of diversity, especially samples from West Bandung which carried the highest diversity. Dominant variants are the VOC B.1.617.2 (Delta) variant, B.1.466.2 variant, and B.1.470 variant. The genomic regions with the highest number of mutations are the spike, NSP3, nucleocapsid, NSP12, and ORF3a protein. Mutation analysis showed that mutations in structural protein might increase the stability of the protein. Oppositely, mutations in non-structural protein might lead to a decrease in protein stability. However, further research to study the impact of mutations on the function of SARS-CoV-2 proteins are required.
Imaging of Virus-Infected Cells with Soft X-ray Tomography
Authors: Garriga Damià , Chichón Francisco Javier , Calisto Bárbara M. , Ferrero Diego S. , Gastaminza Pablo , Pereiro Eva , Pérez-Berna Ana Joaquina
Published: October 2021

Viruses are obligate parasites that depend on a host cell for replication and survival. Consequently, to fully understand the viral processes involved in infection and replication, it is fundamental to study them in the cellular context. Often, viral infections induce significant changes in the subcellular organization of the host cell due to the formation of viral factories, alteration of cell cytoskeleton and/or budding of newly formed particles. Accurate 3D mapping of organelle reorganization in infected cells can thus provide valuable information for both basic virus research and antiviral drug development. Among the available techniques for 3D cell imaging, cryo–soft X-ray tomography stands out for its large depth of view (allowing for 10 µm thick biological samples to be imaged without further thinning), its resolution (about 50 nm for tomographies, sufficient to detect viral particles), the minimal requirements for sample manipulation (can be used on frozen, unfixed and unstained whole cells) and the potential to be combined with other techniques (i.e., correlative fluorescence microscopy). In this review we describe the fundamentals of cryo–soft X-ray tomography, its sample requirements, its advantages and its limitations. To highlight the potential of this technique, examples of virus research performed at BL09-MISTRAL beamline in ALBA synchrotron are also presented.
Self-Reporting of Risk Pathways and Parameter Values for Foot-and-Mouth Disease in Slaughter Cattle from Alternative Production Systems by Kenyan and Ugandan Veterinarians
Authors: Adamchick Julie , Rich Karl , Perez Andres
Published: October 2021

Countries in which foot-and-mouth disease (FMD) is endemic may face bans on the export of FMD-susceptible livestock and products because of the associated risk for transmission of FMD virus. Risk assessment is an essential tool for demonstrating the fitness of one’s goods for the international marketplace and for improving animal health. However, it is difficult to obtain the necessary data for such risk assessments in many countries where FMD is present. This study bridged the gaps of traditional participatory and expert elicitation approaches by partnering with veterinarians from the National Veterinary Services of Kenya (n = 13) and Uganda (n = 10) enrolled in an extended capacity-building program to systematically collect rich, local knowledge in a format appropriate for formal quantitative analysis. Participants mapped risk pathways and quantified variables that determine the risk of infection among cattle at slaughter originating from each of four beef production systems in each country. Findings highlighted that risk processes differ between management systems, that disease and sale are not always independent events, and that events on the risk pathway are influenced by the actions and motivations of value chain actors. The results provide necessary information for evaluating the risk of FMD among cattle pre-harvest in Kenya and Uganda and provide a framework for similar evaluation in other endemic settings.
An Assessment of Hydroacoustic and Electric Fishing Data to Evaluate Long Term Spatial and Temporal Fish Population Change in the River Thames, UK
Authors: Lyons Jim , Hateley Jon , Peirson Graeme , Eley Frances , Manwaring Stuart , Twine Karen
Published: October 2021

This paper reports the results from mobile hydroacoustic surveys carried out between 1994 and 2018, to assess the fish stocks in four impounded reaches, covering 19.8 km of the River Thames, England. The data are complemented with electric fishing boom boat results, collected at the same study reaches and time periods. Hydroacoustic surveys used inter-calibrated dual and split-beam scientific echosounders, with the transducers beaming horizontally across the river to provide fish abundance and distribution estimates. Electric fishing surveys provided catch per unit effort estimates and information on size structure and species composition. Catch data from the margins of the study reaches were dominated by roach (Rutilus rutilus), with bleak (Alburnus alburnus) dominant in mid-river. Hydroacoustic data demonstrated patchy spatial distribution, often associated with natural and anthropogenic habitat features. Cyclical peaks and troughs in both hydroacoustic and electric fishing abundance were found. There were periods of correspondence with electric fishing abundance estimates, but also periods of significant divergence. The concept of ‘Shifting Baseline Syndrome’ is discussed with reference to these data, illustrating the importance of viewing long term quantitative information when using meaningful reference points. The potential impact of river temperature and flow on the fish population data are discussed.