bluuebunny/crossref_metadata_2025 huggingface dataset:
Sustainable Large-Scale Aquaculture of the Northern Hemisphere Sea Lettuce, Ulva fenestrata, in an Off-Shore Seafarm
Authors: Steinhagen Sophie , Enge Swantje , Larsson Karin , Olsson Joakim , Nylund Göran M. , Albers Eva , Pavia Henrik , Undeland Ingrid , Toth Gunilla B.
Published: June 2021

The growing world population demands an increase in sustainable resources for biorefining. The opening of new farm grounds and the cultivation of extractive species, such as marine seaweeds, increases worldwide, aiming to provide renewable biomass for food and non-food applications. The potential for European large-scale open ocean farming of the commercial green seaweed crop Ulva is not yet fully realized. Here we conducted manipulative cultivation experiments in order to investigate the effects of hatchery temperature (10 and 15 °C), nutrient addition (PES and 3xPES) and swarmer density (500 and 10,000 swarmers ml−1) on the biomass yield and biochemical composition (fatty acid, protein, carbohydrate, pigment and phenolic content) of off-shore cultivated Ulva fenestrata in a Swedish seafarm. High seedling densities were optimal for the growth of this northern hemisphere crop strain and significantly increased the mean biomass yield by ~84% compared to low seedling densities. Variations of nutrients or changes in temperature levels during the hatchery phase were not necessary to increase the subsequent growth in an open-water seafarm, however effects of the factors on the thallus habitus (thallus length/width) were observed. We found no significant effect of the environmental factors applied in the hatchery on the total fatty acid or crude protein content in the off-shore cultivated Ulva. However, low seedling density and low temperature increased the total carbohydrate content and furthermore, high temperature in combination with high nutrient levels decreased the pigment content (chlorophyll a, b, carotenoids). Low temperature in combination with high nutrient levels increased the phenolic content. Our study confirms the successful and sustainable potential for large-scale off-shore cultivation of the Scandinavian crop U. fenestrata. We conclude that high seedling density in the hatchery is most important for increasing the total biomass yield of sea-farmed U. fenestrata, and that changing temperature or addition of nutrients overall does not have a large effect on the biochemical composition. To summarize, our study contributes novel insights into the large-scale off-shore cultivation potential of northern hemisphere U. fenestrata and underpins suitable pre-treatments during the hatchery phase of seedlings to facilitate a successful and cost-efficient large-scale rope cultivation.
A Vital Staining Practice That Discerns Ancestry within Groups of Settling Larvae of a Brooding Coral
Authors: Shefy Dor , Shashar Nadav , Rinkevich Baruch
Published: June 2021

Xenogeneic and allogeneic encounters following aggregated and clustered settlements of coral larvae (planulae) may carry important ecological consequences in shaping coral reefs’ communities. However, larval settlement behaviors and settlement location choices in the presence of conspecifics or heterospecifics have not been examined in detail, due to a lack of experimental tools. One potential approach is the employment of vital staining of planulae with dyes that do not impair larval metamorphosis processes, are stable for prolonged periods, and do not diffuse to un-labeled counterpart planulae. For these purposes, we examined the use of neutral red (NR) dye, as an identification marker, on the planulae of Stylophora pistillata, a Red Sea branching coral species. To examine possible NR impacts on larval settlement in the presence of conspecific planulae, we followed the settlement ratios of kin, non-kin, and mixed assemblages, as a proxy for metamorphosis success. We found no differences in settlement rates of stained vs. unstained larvae, lack of stain diffusion to other larvae and that NR stain is maintained for more than a week under a still water regimen. Thus, staining with NR may serve as a useful experimental tool, opening new opportunities in studying larval settlement patterns in sessile marine organisms.
A Eulerian–Lagrangian Coupled Method for the Simulation of Submerged Granular Column Collapse
Authors: Wang Chun , Ye Guanlin , Meng Xiannan , Wang Yongqi , Peng Chong
Published: June 2021

A two-fluid Eulerian–Lagrangian coupled model is developed to investigate the complex interactions between solid particles and the ambient water during the process of submerged granular column collapse. In this model, the water phase is considered to be a Newtonian fluid, whereas the granular column is modeled as an elastic–perfectly plastic material. The water flow field is calculated by the mesh-based Eulerian Finite Volume Method (FVM), with the free surface captured by the Volume-of-Fluid (VOF) technique. The large deformation of the granular material is simulated by the mesh-free, particle-based Lagrangian Smoothed Particle Hydrodynamics method (SPH). Information transfer between Eulerian nodes and Lagrangian particles is performed by the aid of the SPH interpolation function. Both dry and submerged granular column collapses are simulated with the proposed model. Experiments of the submerged cases are also conducted for comparison. Effects of dilatancy (compaction) of initially dense (loose) packing granular columns on the mixture dynamics are investigated to reveal the mechanisms of different flow regimes. Pore water pressure field and granular velocity field are in good agreement between our numerical results and experimental observations, which demonstrates the capability of the proposed Eulerian–Lagrangian coupled method in dealing with complex submerged water–granular mixture flows.
Numerical Study of the Pulsation Process of Spark Bubbles under Three Boundary Conditions
Authors: Ma Chunlong , Shi Dongyan , Li Chao , He Dongze , Li Guangliang , Lu Keru
Published: June 2021

In this study, a compressible three-phase homogeneous model was established using ABAQUS/Explicit. These models can numerically simulate the pulsation process of cavitation bubbles in the free field, near the flat plate target, and near the curved boundary target. At the same time, these models can numerically simulate the strong nonlinear interaction between the cavitation bubble and its nearby wall boundaries. The mutual flow of liquid and gas and fluid solid coupling were solved by the Euler domain in simulation. The results of the numerical simulation were verified by comparing them with the experimental results. In this study, we used electric spark bubbles to represent cavitation bubbles. A high-speed camera was used to record the pulsation process of cavitation bubbles. This study first verified the pulsation process of cavitation bubbles in the free field, because it was the simplest case. Then we verified the interaction process between cavitation bubbles and different wall boundaries. In order to further confirm the credibility of the numerical simulation results, for each wall surface, this study used two burst distances (10 mm and 25 mm) for simulation verification. The numerical model established in this study could effectively simulate the pulsation characteristics of cavitation bubbles, such as the formation of jets and annular bubbles. After verification, the simulated cavitation bubble was almost the same as the cavitation bubble captured by the high-speed camera in the experiment in terms of time, volume, and shape. In this study, a detailed velocity field of the cavitation bubble collapse stage was obtained, which laid down the foundation for the study of the strong nonlinear interaction between the cavitation bubble and the target plates of different shapes. Compared with the experimental results, we found that the numerical model established by the simulation could accurately simulate the bubble pulsation and jet formation processes. In the experiment, the interval time for the bubble pictures taken by the high-speed camera was 41.66 μs per frame. Using a numerical model, the bubble pulsation process can be simulated at an interval of 1 µs per frame. Therefore, the numerical model established by the simulation could show the movement characteristics of the cavitation bubble pulsation process in more detail.
Experimental Investigation on Structural Responses of a Partially Submerged 2D Flat Plate with Hammering and Breaking Waves for Numerical Validation
Authors: Ha Yoon-Jin , Park Byoung-Jae , Kim Yun-Ho , Lee Kang-Su
Published: June 2021

In this study, experiments were conducted to provide validation data for numerical simulations. Model tests were conducted in a 2D wave flume at the Korea Research Institute of Ships and Ocean Engineering (KRISO). A series of hammering tests for two flat plates with different lengths under dry and partially wet conditions were performed to investigate the vibrating frequencies in each mode. Thereafter, breaking wave tests were performed using the focusing wave method. Repetitive tests were performed five times in each condition. The repetitive test results showed good agreement in each case, and the frequencies for each mode of the two flat plates were numerically calculated. In addition, the wave and air bubble frequencies were captured unlike in the hammering tests. The frequencies for each mode, strain and time interval from the experiments for two flat plates were organized, and the data for validation of the numerical simulation were provided.
Biocontrol Agents: Toolbox for the Screening of Weapons against Mycotoxigenic Fusarium
Authors: Pellan Lucile , Dieye Cheikh Ahmeth Tidiane , Durand Noël , Fontana Angélique , Strub Caroline , Schorr-Galindo Sabine
Published: June 2021

The aim of this study was to develop a set of experiments to screen and decipher the mechanisms of biocontrol agents (BCAs), isolated from commercial formulation, against two major mycotoxigenic fungi in cereals, Fusarium graminearum and Fusarium verticillioides. These two phytopathogens produce mycotoxins harmful to human and animal health and are responsible for the massive use of pesticides, for the protection of cereals. It is therefore essential to better understand the mechanisms of action of alternative control strategies such as the use of BCAs in order to optimize their applications. The early and late stages of interaction between BCAs and pathogens were investigated from germination of spores to the effects on perithecia (survival form of pathogen). The analysis of antagonist activities of BCAs revealed different strategies of biocontrol where chronological, process combination and specialization aspects of interactions are discussed. Streptomyces griseoviridis main strategy is based on antibiosis with the secretion of several compounds with anti-fungal and anti-germination activity, but also a mixture of hydrolytic enzymes to attack pathogens, which compensates for an important deficit in terms of spatial colonization capacity. It has good abilities in terms of nutritional competition. Trichoderma asperellum is capable of activating a very wide range of defenses and attacks combining the synthesis of various antifungal compounds (metabolite, enzymes, VOCs), with different targets (spores, mycelium, mycotoxins), and direct action by mycoparasitism and mycophagy. Concerning Pythium oligandrum, its efficiency is mainly due to its strong capacity to colonize the environment, with a direct action via microbial predation, stimulation of its reproduction at the contact of pathogens and the reduction of perithecia formation.
Oncogenic Role of miRNA in Environmental Exposure to Plasticizers: A Systematic Review
Authors: Ferrante Margherita , Cristaldi Antonio , Oliveri Conti Gea
Published: June 2021

The daily environmental exposure of humans to plasticizers may adversely affect human health, representing a global issue. The altered expression of microRNAs (miRNAs) plays an important pathogenic role in exposure to plasticizers. This systematic review summarizes recent findings showing the modified expression of miRNAs in cancer due to exposure to plasticizers. Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) methodology, we performed a systematic review of the literature published in the past 10 years, focusing on the relationship between plasticizer exposure and the expression of miRNAs related to cancer. Starting with 535 records, 17 articles were included. The results support the hypothesis that exposure to plasticizers causes changes in or the deregulation of a number of oncogenic miRNAs and show that the interaction of plasticizers with several redundant miRNAs, such as let-7f, let-7g, miR-125b, miR-134, miR-146a, miR-22, miR-192, miR-222, miR-26a, miR-26b, miR-27b, miR-296, miR-324, miR-335, miR-122, miR-23b, miR-200, miR-29a, and miR-21, might induce deep alterations. These genotoxic and oncogenic responses can eventually lead to abnormal cell signaling pathways and metabolic changes that participate in many overlapping cellular processes, and the evaluation of miRNA-level changes can be a useful target for the toxicological assessment of environmental pollutants, including plastic additives and plasticizers.
What Can We Change in Diet and Behaviour in Order to Decrease Carotid Intima-Media Thickness in Patients with Obesity?
Authors: Rychter Anna Maria , Naskręt Dariusz , Zawada Agnieszka , Ratajczak Alicja Ewa , Dobrowolska Agnieszka , Krela-Kaźmierczak Iwona
Published: June 2021

Atherosclerosis—considered the major cause of cardiovascular diseases (CVDs)—is strongly associated with obesity, to which it strongly contributes. Moreover, atherosclerosis is characterised by a long asymptomatic phase, and its progression can lead to serious cardiovascular (CV) events. The carotid intima-media thickness (cIMT) has been determined as a predictor of CV events, as well as a marker of subclinical atherosclerosis, and has been used in clinical trials as an alternative assessment method or a surrogate endpoint. It should be noted that several behavioural approaches can directly influence the cIMT values, and decrease or increase the CV risk. In our paper, we aimed to summarize the current knowledge regarding IMT measurement among patients with obesity as a risk group—also in terms of the obesity paradox where the diagnosis of subclinical atherosclerosis is especially essential and implements the early therapeutic approach. We also summarized behavioural, modifiable factors, such as the Mediterranean diet, the Dietary Approach to Stop Hypertension Diets, body weight reduction or the intake of micro- and macronutrients, with a particular focus on the studies where the cIMT values were one of the outcomes. In order to collect the literature data related to the presented topic, the PubMed database was explored.
Quantile Risk–Return Trade-Off
Authors: Aslanidis Nektarios , Christiansen Charlotte , Savva Christos S.
Published: June 2021

We investigate the risk–return trade-off on the US and European stock markets. We investigate the non-linear risk–return trade-off with a special eye to the tails of the stock returns using quantile regressions. We first consider the US stock market portfolio. We find that the risk–return trade-off is significantly positive at the upper tail (0.9 quantile), where the upper tail is large positive excess returns. The positive trade-off is as expected from asset pricing models. For the lower tail (0.1 quantile), that is for large negative stock returns, the trade-off is significantly negative. Additionally, for the median (0.5 quantile), the risk–return trade-off is insignificant. These results are recovered for the US industry portfolios and for Eurozone stock market portfolios.
Research Jungle on Online Consumer Behaviour in the Context of Web 2.0: Traceability, Frontiers and Perspectives in the Post-Pandemic Era
Authors: Zhang Xi , Liu Hongda , Yao Pinbo
Published: June 2021

In recent years, the study of online consumption behavior has gradually formed its research system and analysis model based on the inheritance of traditional research paradigms, focusing on the inner mechanism of consumption models explained by consumption activities. Online consumption is based on the research scenario of social e-commerce and forms a broad research network through the extension of consumer objects, consumer psychology, and consumer concepts. Although the theoretical constructs of online consumer behavior continue to improve, the relevant studies still do not fully grasp the research frontiers due to the lagging research nature. In the context of Web 2.0, it is impossible to run through the latest developments in online consumption research. Moreover, the study of online consumer behavior has shown a trend of diversification and multiple schools of thought, and a research jungle has emerged, which in essence is the perfection and new height of the study of consumerism. This paper analyses the origins, frontiers, and prospects of online consumer behavior research to clarify the formation principles, development paths, and future directions of the online consumer behavior research jungle. Ultimately based on the economic changes in the post-pandemic context, this paper integrates and proposes an evolving mechanism for studying online consumption behavior, intending to achieve a peek into and reveal the jungle of online consumption research.
Land Abandonment in Mountain Areas of the EU: An Inevitable Side Effect of Farming Modernization and Neglected Threat to Sustainable Land Use
Authors: Dax Thomas , Schroll Karin , Machold Ingrid , Derszniak-Noirjean Martyna , Schuh Bernd , Gaupp-Berghausen Mailin
Published: June 2021

In a period of rising concern for sustainable land management systems to achieve food security at a global scale, land-use changes demand increased attention. This study assesses the past observations and future risk calculations for land abandonment across European regions, highlighting the particular risk for mountain areas. It draws from a study commissioned by the European Parliament to investigate the situation and probability for high and very high risk of land abandonment until 2030. Revealing that land abandonment is at three times higher risk in mountain areas than in non-mountain areas, the need for action to cope with this pressure is the core result. We reveal that the high disparity in agricultural competitiveness between regions (at fine geographical scale) is the main driving force leading to the spatially uneven performance of land management. Viewing this wide set of drivers and mitigation options, land abandonment is understood as the outcome of a multitude of factors of socio-ecological systems and a combination of farm-specific, internal regional and trans-regional factors. The present dominance of narratives of effectiveness leaves little scope for mountain regions under threat of abandonment and marginalization. In this situation, policy reform would address the issue but this might turn out to be influential only if the complex nature and trade-off of the comprehensive policy framework are prioritized.
Spatial Distribution and Quantification of Mammographic Breast Density, and Its Correlation with BI-RADS Using an Image Segmentation Method
Authors: Goh Yi , Lee Zhen , Lai Christopher
Published: June 2021

(1) Background: Mammographic breast density (MBD) and older age are classical breast cancer risk factors. Normally, MBDs are not evenly distributed in the breast, with different women having different spatial distribution and clustering patterns. The presence of MBDs makes tumors and other lesions challenging to be identified in mammograms. The objectives of this study were: (i) to quantify the amount of MBDs—in the whole (overall), different sub-regions, and different zones of the breast using an image segmentation method; (ii) to investigate the spatial distribution patterns of MBD in different sub-regions of the breast. (2) Methods: The image segmentation method was used to quantify the overall amount of MBDs in the whole breast (overall percentage density (PD)), in 48 sub-regions (regional PDs), and three different zones (zonal PDs) of the whole breast, and the results of the amount of MBDs in 48 sub-regional PDs were further analyzed to determine its spatial distribution pattern in the breast using Moran’s I values (spatial autocorrelation). (3) Results: The overall PD showed a negative correlation with age (p = 0.008); the younger women tended to have denser breasts (higher overall PD in breasts). We also found a higher proportion (p < 0.001) of positive autocorrelation pattern in the less dense breast group than in the denser breast group, suggesting that MBDs in the less dense breasts tend to be clustered together. Moreover, we also observed that MBDs in the mature women (<65 years old) tended to be clustered in the middle zone, while in older women (>64 years old) they tended to be clustered in both the posterior and middle zones. (4) Conclusions: There is an inverse relationship between the amount of MBD (overall PD in the breast) and age, and a different clustering pattern of MBDs between the older and mature women.
Emerging Treatment Options for Multi-Drug-Resistant Bacterial Infections
Authors: Giurazza Roberto , Mazza Maria Civita , Andini Roberto , Sansone Pasquale , Pace Maria Caterina , Durante-Mangoni Emanuele
Published: June 2021

Antimicrobial resistance (AMR) remains one of the top public health issues of global concern. Among the most important strategies for AMR control there is the correct and appropriate use of antibiotics, including those available for the treatment of AMR pathogens. In this article, after briefly reviewing the most important and clinically relevant multi-drug-resistant bacteria and their main resistance mechanisms, we describe the emerging antimicrobial options for both MDR Gram-positive cocci and Gram-negative bacilli, including recently marketed agents, molecules just approved or under evaluation and rediscovered older antibiotics that have regained importance due to their antimicrobial spectrum. Specifically, emerging options for Gram-positive cocci we reviewed include ceftaroline, ceftobiprole, tedizolid, dalbavancin, and fosfomycin. Emerging treatment options for Gram-negative bacilli we considered comprise ceftolozane-tazobactam, ceftazidime-avibactam, meropenem-vaborbactam, imipenem-relebactam, aztreonam-avibactam, minocycline, fosfomycin, eravacycline, plazomicin, and cefiderocol. An exciting scenario is opening today with the long awaited growing availability of novel molecules for the treatment of AMR bacteria. Knowledge of mechanisms of action and resistance patterns allows physicians to increasingly drive antimicrobial treatment towards a precision medicine approach. Strict adherence to antimicrobial stewardship practices will allow us to preserve the emerging antimicrobials for our future.
Engineered Magnetization Dynamics of Magnonic Nanograting Filters
Authors: Yagan Rawana , Katmis Ferhat , Onbaşlı Mehmet C.
Published: June 2021

Magnonic crystals and gratings could enable tunable spin-wave filters, logic, and frequency multiplier devices. Using micromagnetic models, we investigate the effect of nanowire damping, excitation frequency and geometry on the spin wave modes, spatial and temporal transmission profiles for a finite patterned nanograting under external direct current (DC) and radio frequency (RF) magnetic fields. Studying the effect of Gilbert damping constant on the temporal and spectral responses shows that low-damping leads to longer mode propagation lengths due to low-loss and high-frequency excitations are also transmitted with high intensity. When the nanowire is excited with stronger external RF fields, higher frequency spin wave modes are transmitted with higher intensities. Changing the nanowire grating width, pitch and its number of periods helps shift the transmitted frequencies over super high-frequency (SHF) range, spans S, C, X, Ku, and K bands (3–30 GHz). Our design could enable spin-wave frequency multipliers, selective filtering, excitation, and suppression in magnetic nanowires.
Effect of the Alkaline Metal Ion on the Crystal Structure and Magnetic Properties of Heterometallic GdIII-VIV Complexes Based on Cyclobutane-1,1-Dicarboxylate Anions
Authors: Bazhina Evgeniya S. , Korlyukov Alexander A. , Voronina Julia K. , Babeshkin Konstantin A. , Ugolkova Elena A. , Efimov Nikolay N. , Fedin Matvey V. , Kiskin Mikhail A. , Eremenko Igor L.
Published: June 2021

A series of heterometallic GdIII-VIV compounds were synthesized by the reaction of VOSO4·3H2O with cyclobutane-1,1-dicarboxylic acid salts M2(cbdc) (M = Na, Rb, Cs). The new compounds were formed by [Gd(VO)2(cbdc)4(H2O)8]− trinuclear anionic units that were similar in composition but differed in structure, depending on the nature of the alkali metal cation incorporated in the crystal structure of the compound. In the case of Na+, the {GdV2}− units were characterized by identical V···Gd distances and were linked into the 1D-polymeric chain [NaGd(VO)2(cbdc)4(H2O)10]n (1). In the systems with Rb+ and Cs+, the V···Gd distances were different, and the {GdV2}− units were linked into the 3D-framework {[RbGd(VO)2(cbdc)4(H2O)10]·2.5H2O}n (2) and the octanuclear molecule {[CsGd(VO)2(cbdc)4(H2O)11]·5H2O}2 (3), respectively. According to dc-magnetic measurements, the VIV and GdIII ions were ferromagnetically coupled in compound 1 (JVGd = 0.163 ± 0.008 cm−1), while in compounds 2 and 3, ferro- and weak antiferromagnetic exchange interactions were observed (JVGd = 0.989 ± 0.028 and −0.089 ± 0.008 cm−1 for 2, 0.656 ± 0.009 and −0.050 ± 0.004 cm−1 for 3). Analysis of the EPR spectra of 1 revealed the presence of weak magnetic anisotropy of GdIII ions (D ~ 0.08 cm−1 and E/D ~ 0.1–0.15). Ac-susceptibility measurements showed an occurrence the field-induced slow relaxation of magnetization in 1–3.
Going to Extremes: Weakly Supervised Medical Image Segmentation
Authors: Roth Holger R. , Yang Dong , Xu Ziyue , Wang Xiaosong , Xu Daguang
Published: June 2021

Medical image annotation is a major hurdle for developing precise and robust machine-learning models. Annotation is expensive, time-consuming, and often requires expert knowledge, particularly in the medical field. Here, we suggest using minimal user interaction in the form of extreme point clicks to train a segmentation model which, in effect, can be used to speed up medical image annotation. An initial segmentation is generated based on the extreme points using the random walker algorithm. This initial segmentation is then used as a noisy supervision signal to train a fully convolutional network that can segment the organ of interest, based on the provided user clicks. Through experimentation on several medical imaging datasets, we show that the predictions of the network can be refined using several rounds of training with the prediction from the same weakly annotated data. Further improvements are shown using the clicked points within a custom-designed loss and attention mechanism. Our approach has the potential to speed up the process of generating new training datasets for the development of new machine-learning and deep-learning-based models for, but not exclusively, medical image analysis.
A Global Analysis of Delayed SARS-CoV-2/Cancer Model with Immune Response
Authors: Al Agha Afnan , Alshehaiween Safiya , Elaiw Ahmed , Alshaikh Matuka
Published: June 2021

Coronavirus disease 2019 (COVID-19) is a respiratory disease caused by SARS-CoV-2. It appeared in China in late 2019 and rapidly spread to most countries of the world. Cancer patients infected with SARS-CoV-2 are at higher risk of developing severe infection and death. This risk increases further in the presence of lymphopenia affecting the lymphocytes count. Here, we develop a delayed within-host SARS-CoV-2/cancer model. The model describes the occurrence of SARS-CoV-2 infection in cancer patients and its effect on the functionality of immune responses. The model considers the time delays that affect the growth rates of healthy epithelial cells and cancer cells. We provide a detailed analysis of the model by proving the nonnegativity and boundedness of the solutions, finding steady states, and showing the global stability of the different steady states. We perform numerical simulations to highlight some important observations. The results indicate that increasing the time delay in the growth rate of cancer cells reduced the size of tumors and decreased the likelihood of deterioration in the condition of SARS-CoV-2/cancer patients. On the other hand, lymphopenia increased the concentrations of SARS-CoV-2 particles and cancer cells, which worsened the condition of the patient.
Relations between Extrinsic and Intrinsic Invariants of Statistical Submanifolds in Sasaki-Like Statistical Manifolds
Authors: Aytimur Hülya , Mihai Adela , Özgür Cihan
Published: June 2021

The Chen first inequality and a Chen inequality for the δ(2,2)-invariant on statistical submanifolds of Sasaki-like statistical manifolds, under a curvature condition, are obtained.
Protective Effects of Quercetin on Oxidative Stress-Induced Tubular Epithelial Damage in the Experimental Rat Hyperoxaluria Model
Authors: Guzel Ahmet , Yunusoglu Sedat , Calapoglu Mustafa , Candan Ibrahim Aydın , Onaran Ibrahim , Oncu Meral , Ergun Osman , Oksay Taylan
Published: June 2021

Background and Objectives: The most common kidney stones are calcium stones and calcium oxalate (CaOx) stones are the most common type of calcium stones. Hyperoxaluria is an essential risk factor for the formation of these stones. Quercetin is a polyphenol with antioxidant, anti-inflammatory, and many other physiological effects. The aim of this study was to investigate the protective effect of quercetin in hyperoxaluria-induced nephrolithiasis. Materials and Methods: Male Wistar-Albino rats weighing 250–300 g (n = 24) were randomized into three groups: Control (n = 8), ethylene glycol (EG) (n = 8), and EG + quercetin (n = 8). One percent EG-water solution was given to all rats except for the control group as drinking water for five weeks. Quercetin-water solution was given to the EG + quercetin group by oral gavage at a dose of 10 mg/kg/day. Malondialdehyde (MDA), catalase (CAT), urea, calcium, and oxalate levels were analyzed in blood and urine samples. Histopathological assessments and immunohistochemical analyses for oxidative stress and inflammation indicators p38 mitogen-activated protein kinase (p38-MAPK) and nuclear factor kappa B (NF-kB) were performed on renal tissues. Results: The MDA levels were significantly lower in the quercetin-treated group than in the EG-treated group (p = 0.001). Although CAT levels were higher in the quercetin-treated group than the EG-administered group, they were not significantly different between these groups. The expression of p38 MAPK was significantly less in the quercetin-treated group than the EG group (p < 0.004). There was no statistically significant difference between the quercetin and EG groups in terms of NF-kB expression. Conclusions: We conclude that hyperoxaluria activated the signaling pathways, which facilitate the oxidative processes leading to oxalate stone formation in the kidneys. Our findings indicated that quercetin reduced damage due to hyperoxaluria. These results imply that quercetin can be considered a therapeutic agent for decreasing oxalate stone formation, especially in patients with recurrent stones due to hyperoxaluria.
A Comparative Study of Antioxidative Activity of Saliva in Children and Young Teenagers with and without Gingivitis
Authors: Tričković Janjić Olivera , Cvetković Tatjana , Stojković Branislava , Mladenović Raša , Janjić Ranković Mila
Published: June 2021

Objectives: The aim of this study was to compare the values of total antioxidant capacity (TAC), catalase (CAT) and glutathione peroxidase (GPX) in the saliva of children and young teenagers with and without gingivitis. Materials and Methods: A total of 120 children and young teenagers of the mean age of 12.2 participated in the research. Gingival condition was assessed using the Löe and Silness Gingival Index. The subjects were divided into groups of those without gingivitis and those with gingivitis. Samples of unstimulated saliva were collected, and TAC, CAT and GPX were determined spectrophotometrically. Results: By comparing the values of TAC, CAT and GPX in subjects with and without gingivitis, significantly lower values of TAC (p < 0.001) and CAT (p < 0.001) were observed in the group of subjects with gingivitis. The correlation analysis of these values showed a positive correlation in groups of subjects not suffering from gingival inflammation and those with gingival inflammation. Conclusions: The study showed significantly lower values of TAC and CAT in the saliva of subjects with gingivitis. This indicates their possible role as a potential biomarker in the early diagnosis and expression of periodontal disease in children and young teenagers.
A Five-Year Survey for Plastic Surgery Malpractice Claims in Rome, Italy
Authors: Feola Alessandro , Minotti Chiara , Marchetti Daniela , Caricato Marco , Capolupo Gabriella Teresa , Marsella Luigi Tonino , La Monaca Giuseppe
Published: June 2021

(1) Introduction: Medical malpractice claims against both health institutions and physicians are a crucial topic in Italy, as well as in other countries, particularly regarding civil proceedings. Our study reports an analysis of all of the malpractice judgments concerning plastic surgery decided in the Civil Court of Rome between 2012 and 2016. (2) Methods: the database of the Observatory Project on Medical Responsibility (ORMe) was analyzed, which collects all of the judgments of the Civil Court of Rome, that is, the first instance district court. Therefore, neither the jurisprudence of the second level court nor that of the Supreme Court was taken into account. (3) Results: 144 judgments concerning plastic surgery were delivered in the five-year period of 2012–2016 (corresponding to 10.6% of total professional liability verdicts of the Civil Court of Rome in the same period). In 101/144 cases (70.14%), the claim was accepted. A total of €4,727,579.00 was paid in compensation for plastic surgery malpractice claims, with a range from a minimum amount of €1555.96 to a maximum amount of €1,425,155.00 and an average compensation of €46,807.71 per claim that was significantly lower compared to other surgical disciplines. (4) Conclusions: Our data confirm that the analyzed branch has a high litigation rate, with a prevalence of convictions for cosmetic procedures over reconstructive ones, both for malpractice and for violation of the informed consent. Plastic surgery is also confirmed among those branches in which the professionals are more frequently sued compared to health institutions.
A Decade of Wishes-Changes in Maternal Preference of the Mode of Delivery among Polish Women over the Last Decade
Authors: Jodzis Agnieszka , Walędziak Maciej , Czajkowski Krzysztof , Różańska-Walędziak Anna
Published: June 2021

Background and Objectives: The maternal preference of mode of delivery is an important problem in respect of patient’s autonomy and shared decision-making. The objective of the study was to obtain information about women’s preferences of the mode of delivery and knowledge about the cesarean section and its’ consequences. Materials and Methods: The study was based on a survey filled in by 1175 women in 2010 and 1033 women in 2020. Respondents were asked about their preference of mode of delivery, possible factors influencing their decision and their knowledge about risks and benefits of cesarean section. Results: There was a significant increase in the rate of women who declared cesarean section as their preferred mode of delivery, from 43.97% in 2010 to 56.03% in 2020 (p < 0.05). In 2010 26.51% of women thought that choice of mode of delivery should be their autonomic decision, 46.36% preferred decision-sharing with their obstetrician, 25.64% thought that cesarean section should be performed for medical indications only (respectively 34.86%, 44.45% and 19.38% in 2020). Conclusions: There has been a significant increase in the rate of Polish women who prefer cesarean delivery over the last decade, as well as in the rate of women who consider the mode of delivery as their autonomic decision.
The Emergence of SARS-CoV-2 Variant(s) and Its Impact on the Prevalence of COVID-19 Cases in the Nabatieh Region, Lebanon
Authors: Noureddine Fatima Y. , Chakkour Mohamed , El Roz Ali , Reda Jana , Al Sahily Reem , Assi Ali , Joma Mohamed , Salami Hassan , Hashem Sadek J. , Harb Batoul , Salami Ali , Ghssein Ghassan
Published: June 2021

Background: An outbreak of an unknown respiratory illness caused by a novel coronavirus, SARS-CoV-2, emerged in the city of Wuhan in Hubei Province, China, in December 2019 and was referred to as coronavirus disease-2019 (COVID-19). Soon after, it was declared as a global pandemic by the World Health Organization (WHO) in March 2020. SARS-CoV-2 mainly infects the respiratory tract with different outcomes ranging from asymptomatic infection to severe critical illness leading to death. Different SARS-CoV-2 variants are emerging of which three have raised concerns worldwide due to their high transmissibility among populations. Objective: To study the prevalence of COVID-19 in the region of Nabatieh-South Lebanon during the past year and assess the presence of SARS-CoV-2 variants and their effect on the spread of infection during times of lockdown. Methods: In our study, 37,474 nasopharyngeal swab samples were collected and analyzed for the detection of SARS-CoV-2 virus in suspected patients attending a tertiary health care center in South Lebanon during the period between 16 March 2020 and 21 February 2021. Results: The results demonstrated a variation in the prevalence rates ranging from less than 1% during full lockdown of the country to 8.4% upon easing lockdown restrictions and reaching 27.5% after the holidays and 2021 New Year celebrations. Interestingly, a new variant(s) appeared starting January 2021 with a significant positive association between the prevalence of positive tests and the percentage of the variant(s). Conclusions: Our results indicate that the lockdown implemented by the Lebanese officials was an effective intervention to contain COVID-19 spread. Our study also showed that lifting lockdown measures during the holidays, which allowed indoor crowded gatherings to occur, caused a surge in COVID-19 cases and rise in the mortality rates nationwide. More importantly, we confirmed the presence of a highly transmissible SARS-CoV-2 variant(s) circulating in the Lebanese community from at least January 2021 onwards.
Correction: Xue, S.; Shen, R. Corrosion-Fatigue Analysis of High-Strength Steel Wire by Experiment and the Numerical Simulation. Metals 2020, 10, 734
Authors: Xue Songling , Shen Ruili
Published: June 2021

The authors wish to make the following corrections to this paper [...]
Study of Metallic Housing of the Adder Gearbox to Reduce the Noise and to Improve the Design Solution
Authors: Gillich Nicoleta , Sîrbu Nicolae , Vlase Sorin , Marin Marin
Published: June 2021

In the manufacture of commercial trucks, used in oil installations or the army, two identical engines are used on a single chassis, whose power is summed by a gearbox, a compact metal construction, which must meet multiple operating requirements. The paper studies the behavior of such an adding box, currently used in manufacturing, and an improved, welded solution that produces less noise and has a lower weight. The finite element method is used for modeling the gearbox in order to analyze stresses and strains and obtain a modal analysis of the system. The results obtained from the calculation are then verified by experimental measurements. The two versions are analyzed in parallel to highlight the advantages of the second version.
Aquatic Ecosystems of the Anthropocene: Limnology and Microbial Ecology of Mine Pit Lakes
Authors: Blanchette Melanie L. , Lund Mark A.
Published: June 2021

Mine pit lakes (‘pit lakes’) are new aquatic ecosystems of the Anthropocene. Potentially hundreds of meters deep, these lakes are prominent in the landscape and in the public consciousness. However, the ecology of pit lakes is underrepresented in the literature. The broad goal of this research was to determine the environmental drivers of pelagic microbe assemblages in Australian coal pit lakes. The overall experimental design was four lakes sampled three times, top and bottom, in 2019. Instrument chains were installed in lakes and measurements of in situ water quality and water samples for metals, metalloids, nutrients and microbe assemblage were collected. Lakes were monomictic and the timing of mixing was influenced by high rainfall events. Water quality and microbial assemblages varied significantly across space and time, and most taxa were rare. Lakes were moderately saline and circumneutral; Archeans were not prevalent. Richness also varied by catchment. Microbial assemblages correlated to environmental variables, and no one variable was consistently significant, spatially or temporally. Study lakes were dominated by ‘core’ taxa exhibiting temporal turnover likely driven by geography, water quality and interspecific competition, and the presence of water chemistry associated with an artificial aquifer likely influenced microbial community composition. Pit lakes are deceptively complex aquatic ecosystems that host equally complex pelagic microbial communities. This research established links between microbial assemblages and environmental variables in pit lakes and determined core communities; the first steps towards developing a monitoring program using microbes.
Legionella and Biofilms—Integrated Surveillance to Bridge Science and Real-Field Demands
Authors: Pereira Ana , Silva Ana Rosa , Melo Luis F.
Published: June 2021

Legionella is responsible for the life-threatening pneumonia commonly known as Legionnaires’ disease or legionellosis. Legionellosis is known to be preventable if proper measures are put into practice. Despite the efforts to improve preventive approaches, Legionella control remains one of the most challenging issues in the water treatment industry. Legionellosis incidence is on the rise and is expected to keep increasing as global challenges become a reality. This puts great emphasis on prevention, which must be grounded in strengthened Legionella management practices. Herein, an overview of field-based studies (the system as a test rig) is provided to unravel the common roots of research and the main contributions to Legionella’s understanding. The perpetuation of a water-focused monitoring approach and the importance of protozoa and biofilms will then be discussed as bottom-line questions for reliable Legionella real-field surveillance. Finally, an integrated monitoring model is proposed to study and control Legionella in water systems by combining discrete and continuous information about water and biofilm. Although the successful implementation of such a model requires a broader discussion across the scientific community and practitioners, this might be a starting point to build more consistent Legionella management strategies that can effectively mitigate legionellosis risks by reinforcing a pro-active Legionella prevention philosophy.
Development of an Optimized Clearing Protocol to Examine Adipocyte Subpopulations in White Adipose Tissue
Authors: Luong Quyen Vi , Israel Andreas , Sharma Rita , Ussar Siegfried , Lee Kevin Y.
Published: June 2021

Organic solvent dibenzyl ether (DBE)-based protocols have been widely used in adipose tissue clearing. However, benzyl alcohol/benzyl benzoate (BABB)-based clearing has been shown to offer better transparency in other tissues. The addition of diphenyl ether (DPE) to BABB (BABB-D4) is often included to preserve fluorescent signals, but its effects on adipose tissue transparency and shrinkage have not been explored. Distinct adipocyte subpopulations contribute to its cellular composition and biological activity. Here, we compared clearing solvents to create an optimized clearing methodology for the study of adipocyte subpopulations. Adipose tissues were cleared with BABB, BABB-D4, and DBE, and post-clearing transparency and tissue shrinkage were measured. An optimized protocol, including BABB-D4 clearing, delipidation, and extensive immunofluorescence blocking steps, was created to examine the spatial distribution of Wt-1 positive progenitor-derived (Type-1) adipocytes in intact mesenteric fat. Both BABB and BABB-D4 lead to significantly increased tissue transparency with reduced tissue shrinkage compared to DBE-cleared adipose tissue. Type-1 adipocytes are found in a clustered distribution with predominant residence in fat associated with the ileum and colon. This paper details an optimized clearing methodology for adipose tissue with increased tissue transparency and reduced shrinkage, and therefore will be a useful tool for investigating adipose tissue biology.
Status and Perspectives of 2ϵ, ϵβ+ and 2β+ Decays
Authors: Belli Pierluigi , Bernabei Rita , Caracciolo Vincenzo
Published: June 2021

This paper reviews the main experimental techniques and the most significant results in the searches for the 2ϵ, ϵβ+ and 2β+ decay modes. Efforts related to the study of these decay modes are important, since they can potentially offer complementary information with respect to the cases of 2β− decays, which allow a better constraint of models for the nuclear structure calculations. Some positive results that have been claimed will be mentioned, and some new perspectives will be addressed shortly.
Evaluation of Filter, Paramagnetic, and STAGETips Aided Workflows for Proteome Profiling of Symbiodiniaceae Dinoflagellate
Authors: Supasri Kanoknate , Kumar Manoj , Mathew Mano , Signal Bethany , Padula Matthew , Suggett David , Ralph Peter
Published: June 2021

The integrity of coral reef ecosystems worldwide rests on a fine-tuned symbiotic interaction between an invertebrate and a dinoflagellate microalga from the family Symbiodiniaceae. Recent advances in bottom-up shotgun proteomic approaches and the availability of vast amounts of genetic information about Symbiodiniaceae have provided a unique opportunity to better understand the molecular mechanisms underpinning the interactions of coral-Symbiodiniaceae. However, the resilience of this dinoflagellate cell wall, as well as the presence of polyanionic and phenolics cell wall components, requires the optimization of sample preparation techniques for successful implementation of bottom-up proteomics. Therefore, in this study we compare three different workflows—filter-aided sample preparation (FASP), single-pot solid-phase-enhanced sample preparation (SP3), and stop-and-go-extraction tips (STAGETips, ST)—to develop a high-throughput proteotyping protocol for Symbiodiniaceae algal research. We used the model isolate Symbiodinium tridacnidorum. We show that SP3 outperformed ST and FASP with regard to robustness, digestion efficiency, and contaminant removal, which led to the highest number of total (3799) and unique proteins detected from 23,593 peptides. Most of these proteins were detected with ≥2 unique peptides (73%), zero missed tryptic peptide cleavages (91%), and hydrophilic peptides (>70%). To demonstrate the functionality of this optimized SP3 sample preparation workflow, we examined the proteome of S. tridacnidorum to better understand the molecular mechanism of peridinin-chlorophyll-protein complex (PCP, light harvesting protein) accumulation under low light (LL, 30 μmol photon m−2 s−1). Cells exposed to LL for 7 days upregulated various light harvesting complex (LHCs) proteins through the mevalonate-independent pathway; proteins of this pathway were at 2- to 6-fold higher levels than the control of 120 μmol photon m−2 s−1. Potentially, LHCs which were maintained in an active phosphorylated state by serine/threonine-protein kinase were also upregulated to 10-fold over control. Collectively, our results show that the SP3 method is an efficient high-throughput proteotyping tool for Symbiodiniaceae algal research.