bluuebunny/crossref_metadata_2025 huggingface dataset:
Decomposition and Variation in Carbon and Nitrogen of Leaf Litter Mixtures in a Subtropical Mangrove Forest
Authors: Wang Yi , Li Danyang , Lu Zhiqiang , Ma Li
Published: April 2024

The decomposition of mangrove litter plays a crucial role in material circulation and energy flow within mangrove forests. Evaluating the decomposition-based variation in biogenic elements in litter is important for improving our understanding about their biogeochemical cycling in ecosystems. The main objective of this study was to examine the interaction effect during the decomposition process of mixed Kandelia obovata and Avicennia marina litter. Variations in C and N were also determined in the decomposing leaf litter mixtures. Our findings revealed that the decomposition rates were faster in summer than in winter, and increased with the proportion of A. marina litter. After 35 days of decomposition in summer, the remaining weights for different proportions of K. obovata (KO) and A. marina (AM) were 22.9% (KO:AM = 1:2), 27.2% (KO:AM = 1:1), and 31.2% (KO:AM = 2:1), respectively. Similarly, after 49 days of decomposition in winter, the remaining weights for the different KO:AM proportions were 27.7%, 35.4%, and 44.0%, respectively. Additionally, the decomposition of mixed K. obovata and A. marina litter had an influence on C content and N release dynamics. These results provide a scientific basis for understanding the decomposition of mixed mangrove litter and its implications for material circulation and energy flow within these ecosystems.
Task Allocation of Heterogeneous Multi-Unmanned Systems Based on Improved Sheep Flock Optimization Algorithm
Authors: Liu Haibo , Liao Yang , Shi Changting , Shen Jing
Published: April 2024

The objective of task allocation in unmanned systems is to complete tasks at minimal costs. However, the current algorithms employed for coordinating multiple unmanned systems in task allocation tasks frequently converge to local optima, thus impeding the identification of the best solutions. To address these challenges, this study builds upon the sheep flock optimization algorithm (SFOA) by preserving individuals eliminated during the iterative process within a prior knowledge set, which is continuously updated. During the reproduction phase of the algorithm, this prior knowledge is utilized to guide the generation of new individuals, preventing their rapid reconvergence to local optima. This approach aids in reducing the frequency at which the algorithm converges to local optima, continually steering the algorithm towards the global optimum and thereby enhancing the efficiency of task allocation. Finally, various task scenarios are presented to evaluate the performances of various algorithms. The results show that the algorithm proposed in this paper is more likely than other algorithms to escape from local optima and find the global optimum.
Perspectives of Young Digital Natives on Digital Marketing: Exploring Annoyance and Effectiveness with Eye-Tracking Analysis
Authors: Balaskas Stefanos , Kotsari Georgia , Rigou Maria
Published: April 2024

Currently, there are a wide range of approaches to deploying digital ads, with advanced technologies now being harnessed to craft advertising that is engaging and even tailored to personal interests and preferences, yet potentially distracting and irritating. This research seeks to evaluate contemporary digital advertising methods by assessing how annoying they are to users, particularly when they distract users from intended tasks or cause delays in regular online activities. To pursue this, an eye-tracking study was conducted, with 51 participants navigating a specially designed website featuring seven distinct types of advertisements without a specific content to avoid the effect of ad content on the collected data. Participants were asked to execute specific information-seeking tasks during the experiment and afterwards to report if they recalled seeing each ad and the degree of annoyance by each ad type. Ad effectiveness is assessed by eye-tracking metrics (time to first fixation, average fixation duration, dwell time, fixation count, and revisit count) depicting how appealing an ad is as a marketing stimulus. Findings indicated that pop-ups, ads with content reorganization, and non-skippable videos ranked as the most annoying forms of advertising. Conversely, in-content ads without content reorganization, banners, and right rail ads were indicated as less intrusive options, seeming to strike a balance between effectiveness and user acceptance.
Multi-WiIR: Multi-User Identity Legitimacy Authentication Based on WiFi Device
Authors: Wei Zhongcheng , Dong Yanhu
Published: April 2024

With the proliferation of WiFi devices, WiFi-based identification technology has garnered attention in the security domain and has demonstrated initial success. Nonetheless, when untrained illegitimate users appear, the classifier tends to categorize them as if they were trained users. In response to this issue, researchers have proposed identity legitimacy authentication systems to identify illicit users, albeit only applicable to individual users. In this article, we propose a multi-user legitimacy authentication system based on WiFi, termed Multi-WiIR. Leveraging WiFi signals, the system captures users’ walking patterns to ascertain their legitimacy. The core concept entails training a multi-branch deep neural network, designated WiIR-Net, for feature extraction of individual users. Binary classifiers are then applied to each user, and legitimacy is established by comparing the model’s output to predefined thresholds, thus facilitating multi-user legitimacy authentication. Moreover, the study experimentally investigated the impact of the number of legitimate individuals on accuracy rates. The results demonstrated that The Multi-WiIR system showed commendable performance with low latency, being capable of conducting legitimacy recognition in scenarios involving up to four users, with an accuracy rate reaching 85.11%.
A Survey on Energy-Aware Security Mechanisms for the Internet of Things
Authors: He Peixiong , Zhou Yi , Qin Xiao
Published: April 2024

The Internet of Things (IoT) employs sensors and the Internet for information exchange, enabling intelligent identification, monitoring, and management, which has deeply impacted various sectors such as power, medical care, and security, transforming social activities and lifestyles. Regrettably, IoT systems suffer from two main challenges, namely sustainability and security. Hence, pondering how to enhance sustainable and energy-efficient practices for IoT systems to mitigate risks becomes a worthwhile endeavor. To address this issue, we conduct a survey of energy-aware security mechanisms in the Internet of Things. Specifically, we examine the challenges that IoT is facing in terms of energy efficiency and security, and we inspect current energy-saving and privacy-preserving technologies for IoT systems. Moreover, we delineate a vision for the future of IoT, emphasizing energy-aware security mechanisms. Finally, we outline the challenges encountered in achieving energy-aware security mechanisms, as well as the direction of future research. Motivated by this study, we envision advancements in the IoT that not only harness the benefits of science and technology but also enhance the security and safety of our data.
Intelligent Rapid Detection Techniques for Low-Content Components in Fruits and Vegetables: A Comprehensive Review
Authors: Xu Sai , Guo Yinghua , Liang Xin , Lu Huazhong
Published: April 2024

Fruits and vegetables are an important part of our daily diet and contain low-content components that are crucial for our health. Detecting these components accurately is of paramount significance. However, traditional detection methods face challenges such as complex sample processing, slow detection speed, and the need for highly skilled operators. These limitations fail to meet the growing demand for intelligent and rapid detection of low-content components in fruits and vegetables. In recent years, significant progress has been made in intelligent rapid detection technology, particularly in detecting high-content components in fruits and vegetables. However, the accurate detection of low-content components remains a challenge and has gained considerable attention in current research. This review paper aims to explore and analyze several intelligent rapid detection techniques that have been extensively studied for this purpose. These techniques include near-infrared spectroscopy, Raman spectroscopy, laser-induced breakdown spectroscopy, and terahertz spectroscopy, among others. This paper provides detailed reports and analyses of the application of these methods in detecting low-content components. Furthermore, it offers a prospective exploration of their future development in this field. The goal is to contribute to the enhancement and widespread adoption of technology for detecting low-content components in fruits and vegetables. It is expected that this review will serve as a valuable reference for researchers and practitioners in this area.
The Probiotic Kluyveromyces lactis JSA 18 Alleviates Obesity and Hyperlipidemia in High-Fat Diet C57BL/6J Mice
Authors: Hong Yingxiang , Song Guodong , Feng Xiaoqian , Niu Jialei , Wang Lu , Yang Caini , Luo Xuegang , Zhou Sa , Ma Wenjian
Published: April 2024

Obesity poses a significant threat to various health conditions such as heart diseases, diabetes, high blood pressure, and heart attack, with the gut microbiota playing a crucial role in maintaining the body’s energy balance. We identified a novel probiotic fungal strain, Kluyveromyces lactis JSA 18 (K. lactis), which was isolated from yak milk and was found to possess anti-obesity properties. Additionally, Lactobacillus plantarum CGMCC 8198 (LP8198) from our previous study was also included to evaluate its anti-obesity properties. The findings indicated that K. lactis caused a notable reduction in weight gain, liver and fat indexes, and hyperlipidemia in mice fed a high-fat diet (HFD). Administering K. lactis and LP8198 to mice on a high-fat diet resulted in a reduction of serum triglyceride levels. Furthermore, the supplements reduced ALT and AST activity, and inhibited the production of inflammatory cytokines such as TNF-α and IL-1β. In addition, lipid metabolism was enhanced by the downregulation of ACC1, PPAR-γ, SREBP-1, and Fasn. Moreover, this study found that K. lactis and LP8198 have little effect on gut bacteria. Additionally, K. lactis partially influenced intestinal fungi, while LP8198 had a minor influence on gut mycobiota. The main goal of this research was to show how effective K. lactis can be as a probiotic in combating obesity.
Experimental Study on the Butterfly Shape of the Plastic Zone around a Hole near Rock Failure
Authors: Zhang Wenlong , Yu Jiajia , Ren Jianju , Li Chen , Ma Ji
Published: April 2024

The precursor of rock failure around a hole has always been one of the research hotspots in the field of rock mechanics, and the distribution of the plastic zone is often adopted to reflect the location and form of rock failure. The shape of the plastic zone around a hole before rock failure can guide the mechanism of and early warning methods for geotechnical engineering disasters, while previous theoretical research and numerical simulation results show that the shape of the plastic zone around the hole is butterfly shaped under specific stress, which is referred to as butterfly failure theory. Studies also indicate that the butterfly shape of the plastic zone around a hole is considered to be the main cause of many disasters, which signifies the importance of studying the morphology of the plastic zone near rock failure. Therefore, this study is committed to finding the specific shape of the plastic zone near rock failure through relatively accurate and a high number of AE event location results, and the final experimental results show that the plastic zone around the hole is basically a butterfly shape near rock failure. This study verifies the correctness of the butterfly failure theory and provides an important reference for the study of geotechnical engineering disaster mechanisms and monitoring methods. The fact that the plastic zone in the early stage of rock failure in this study tends to be butterfly shaped preliminarily indicates the fractal law of rock failure. In the moment before rock failure, the distribution of AE events is more regular, which leads to large-scale collapse type failure.
Dynamic Awareness and Strategic Adaptation in Cybersecurity: A Game-Theory Approach
Authors: Kostelić Katarina
Published: April 2024

Awareness and human factors are becoming ever more important in cybersecurity, particularly in the context of small companies that may need more resources to deal with cybersecurity effectively. This paper introduces a theoretical framework for game analysis of the role of awareness in strategic interactions between the manager and a hacker. A computable approach is proposed based on Bayesian updating to model awareness in a cybersecurity context. The process of gaining awareness considers the manager’s perception of the properties of the hacker’s actions, game history, and common knowledge. The role of awareness in strategy choices and outcomes is analyzed and simulated, providing insights into decision-making processes for managers and highlighting the need to consider probabilistic assessments of threats and the effectiveness of countermeasures. The accuracy of the initial frequencies plays a significant role in the manager’s success, with aligned frequencies leading to optimal results. Inaccurate information on prior frequencies still outperforms complete uncertainty, emphasizing the value of any available intelligence. However, the results suggest that other awareness modeling approaches are necessary to enhance the manager’s agility and adaptiveness when the prior frequencies do not reflect the immediate attacker’s type, indicating the need for improved intelligence about cyber-attacks and examinations of different awareness modeling approaches.
Comparative Analysis of Hydrogel Adsorption/Desorption with and without Surfactants
Authors: Dhahir Salam Abdulla , Braihi Auda Jabbar , Habeeb Salih Abbas
Published: April 2024

In this particular study, a hydrogel known as SAP-1 was synthesized through the grafting of acrylic acid-co-acrylamide onto pullulan, resulting in the creation of Pul-g-Poly (acrylic acid-co-acrylamide). Additionally, a sponge hydrogel named SAP-2 was prepared by incorporating the surfactant sodium dodecyl benzene sulfonate (SDBS) into the hydrogel through free radical solution polymerization. To gain further insight into the composition and properties of the hydrogels, various techniques, such as Fourier transform infrared spectroscopy, hydrogen nuclear magnetic resonance (1H NMR), atomic absorption spectroscopy, and field emission scanning electron microscopy (FE-SEM), were employed. Conversely, the absorption kinetics and the equilibrium capacities of the prepared hydrogels were investigated and analyzed. The outcomes of the investigation indicated that each of the synthesized hydrogels exhibited considerable efficacy as adsorbents for cadmium (II), copper (II), and nickel (II) ions. In particular, SAP-2 gel displayed a remarkable cadmium (II) ion absorption ability, with a rate of 190.72 mg/g. Following closely, SAP-1 gel demonstrated the ability to absorb cadmium (II) ions at a rate of 146.9 mg/g and copper (II) ions at a rate of 154 mg/g. Notably, SAP-2 hydrogel demonstrated the ability to repeat the adsorption–desorption cycles three times for cadmium (II) ions, resulting in absorption capacities of 190.72 mg/g, 100.43 mg/g, and 19.64 mg/g for the first, second, and third cycles, respectively. Thus, based on the abovementioned results, it can be concluded that all the synthesized hydrogels possess promising potential as suitable candidates for the adsorption and desorption of cadmium (II), copper (II), and nickel (II) ions.
Therapeutic Management and Outcomes of Hepatoblastoma in a Pediatric Patient with Mosaic Edwards Syndrome
Authors: Sosnowska-Sienkiewicz Patrycja , Kamińska Alicja , Anderko Iwona , Telman-Kołodziejczyk Gabriela , Mańkowski Przemysław , Januszkiewicz-Lewandowska Danuta
Published: April 2024

The mosaic form of Edwards syndrome affects 5% of all children with Edwards syndrome. The clinical phenotype is highly variable, ranging from the full spectrum of trisomy 18 to the normal phenotype. The purpose of this publication was to present the therapeutic process in an 18-month-old girl with the mosaic form of Edwards syndrome and hepatoblastoma, against the background of other cases of simultaneous occurrence of this syndrome and hepatoblastoma described so far. It appears that this particular group of patients with hepatoblastoma and Edwards syndrome can have good outcomes, provided they do not have life-threatening cardiac or other severe defects. Due to the prematurity of our patient and the defects associated with Edwards syndrome, the child required constant multidisciplinary care, but Edwards syndrome itself was not a reason to discontinue therapy for a malignant neoplasm of the liver. Regular abdominal ultrasound examination, along with AFP testing, may be helpful in the early detection of liver tumors in children with Edwards syndrome
Different Nuclear Architecture in Human Sperm According to Their Morphology
Authors: Cassuto Nino-Guy , Ogal Nesrine , Assou Said , Ruoso Lea , Rogers Eli-Jonathan , Monteiro Miguel-José , Thomas Daniel , Siffroi Jean-Pierre , Rouen Alexandre
Published: April 2024

Human sperm parameters serve as a first step in diagnosing male infertility, but not in determining the potential for successful pregnancy during assisted reproductive technologies (ARTs) procedures. Here, we investigated the relationship between sperm head morphology at high magnification, based on strict morphologic criteria, and the nuclear architecture analyzed by fluorescence in situ hybridization (FISH). We included five men. Two of them had an elevated high-magnification morphology score of 6 points (Score 6) indicating high fertility potential, whereas three had a low score of 0 points (Score 0), indicating low fertility potential. We used FISH to study the inter-telomeric distance and the chromosomal territory area of chromosome 1 (Chr. 1). We then compared these two parameters between subjects with high and low scores. FISH data analysis showed that the inter-telomeric distance (ITD) and chromosomal territory area (CTA) of Chr. 1 were significantly higher in subjects with low scores (score 0) than high scores (score 6). Our results suggest that (i) there is a link between nuclear architecture and sperm head abnormalities, particularly vacuoles; and (ii) it is possible to select spermatozoa with normal nuclear architecture, which might indirectly explain the positive ART outcomes observed with this technique.
Review of Personalized Medicine and Pharmacogenomics of Anti-Cancer Compounds and Natural Products
Authors: Zhou Yalan , Peng Siqi , Wang Huizhen , Cai Xinyin , Wang Qingzhong
Published: April 2024

In recent years, the FDA has approved numerous anti-cancer drugs that are mutation-based for clinical use. These drugs have improved the precision of treatment and reduced adverse effects and side effects. Personalized therapy is a prominent and hot topic of current medicine and also represents the future direction of development. With the continuous advancements in gene sequencing and high-throughput screening, research and development strategies for personalized clinical drugs have developed rapidly. This review elaborates the recent personalized treatment strategies, which include artificial intelligence, multi-omics analysis, chemical proteomics, and computation-aided drug design. These technologies rely on the molecular classification of diseases, the global signaling network within organisms, and new models for all targets, which significantly support the development of personalized medicine. Meanwhile, we summarize chemical drugs, such as lorlatinib, osimertinib, and other natural products, that deliver personalized therapeutic effects based on genetic mutations. This review also highlights potential challenges in interpreting genetic mutations and combining drugs, while providing new ideas for the development of personalized medicine and pharmacogenomics in cancer study.
Genetic Modifiers of Sickle Cell Anemia Phenotype in a Cohort of Angolan Children
Authors: Ginete Catarina , Delgadinho Mariana , Santos Brígida , Miranda Armandina , Silva Carina , Guerreiro Paulo , Chimusa Emile R. , Brito Miguel
Published: April 2024

The aim of this study was to identify genetic markers in the HBB Cluster; HBS1L-MYB intergenic region; and BCL11A, KLF1, FOX3, and ZBTB7A genes associated with the heterogeneous phenotypes of Sickle Cell Anemia (SCA) using next-generation sequencing, as well as to assess their influence and prevalence in an Angolan population. Hematological, biochemical, and clinical data were considered to determine patients’ severity phenotypes. Samples from 192 patients were sequenced, and 5,019,378 variants of high quality were registered. A catalog of candidate modifier genes that clustered in pathophysiological pathways important for SCA was generated, and candidate genes associated with increasing vaso-occlusive crises (VOC) and with lower fetal hemoglobin (HbF) were identified. These data support the polygenic view of the genetic architecture of SCA phenotypic variability. Two single nucleotide polymorphisms in the intronic region of 2q16.1, harboring the BCL11A gene, are genome-wide and significantly associated with decreasing HbF. A set of variants was identified to nominally be associated with increasing VOC and are potential genetic modifiers harboring phenotypic variation among patients. To the best of our knowledge, this is the first investigation of clinical variation in SCA in Angola using a well-customized and targeted sequencing approach.
Detection of Macrolide and/or Fluoroquinolone Resistance Genes in Mycoplasma genitalium Strains Isolated from Men in the Northwest Region of Croatia in 2018–2023
Authors: Ljubin-Sternak Sunčanica , Meštrović Tomislav , Marijan Tatjana , Anušić Maja , Šuto Sandra , Vraneš Jasmina
Published: April 2024

Mycoplasma genitalium (M. genitalium) poses a significant public health challenge due to its association with non-gonococcal urethritis (particularly in men) and antimicrobial resistance. However, despite the prevalence of M. genitalium infections and the rise in resistance rates, routine testing and surveillance remain limited. This is the first study from Croatia that aimed to assess the prevalence and trends of resistance in M. genitalium strains isolated from male individuals by detecting macrolide and fluoroquinolone resistance genes. The study also aimed to explore the factors associated with resistance and changes in resistance patterns over time. Urine samples collected from male individuals in the Zagreb County and northwest region of Croatia between 2018 and 2023 were tested for M. genitalium with the use of molecular methods. Positive samples were subjected to DNA extraction and multiplex tandem polymerase chain reaction (MT-PCR) targeting genetic mutations associated with macrolide (23S rRNA gene) and fluoroquinolone (parC gene) resistance. Of the 8073 urine samples tested from 6480 male individuals (and following the exclusion of repeated specimens), we found that the prevalence of M. genitalium infection was 2.2%. Macrolide resistance was observed in 60.4% of strains, while fluoroquinolone resistance was found in 19.2%. Co-resistance to both antibiotics was present in 18.2% of cases. A statistically significant increase in fluoroquinolone resistance was noted over the study period (p = 0.010), but this was not evident for azithromycin resistance (p = 0.165). There were no statistically significant differences in resistance patterns between age groups, whereas re-testing of patients revealed dynamic changes in resistance profiles over time. The high burden of macrolide resistance and increasing fluoroquinolone resistance underscore the urgent need for comprehensive resistance testing and surveillance programs. The implementation of resistance-guided treatment strategies, along with enhanced access to molecular diagnostics, is pivotal for effectively managing M. genitalium infections.
Nonlinear 3D Finite Element Analysis of a Coupled Soil–Structure System by a Deterministic Approach
Authors: Castelli Francesco , Grasso Salvatore , Lentini Valentina , Sammito Maria Stella Vanessa
Published: April 2024

Fully coupled soil–structure analyses were performed for a building of strategic importance located in the city of Messina (Sicily, Italy). The structure was built after the destructive 1908 earthquake, also known as the ‘Messina and Reggio Calabria earthquake’, which caused severe ground shaking. A parametric study considering three seismograms of this earthquake was performed. Deep in situ and laboratory investigations allowed the definition of the geometric and geotechnical model of the subsoil. Numerical analyses were performed with PLAXIS3D finite element software (Version 21.01.00.479). The Hardening Soil model with small-strain stiffness was accurately calibrated using laboratory and field data. The dynamic response was investigated in terms of accelerations, response spectra, amplification functions, displacements and stress–strain hysteretic loops. The findings show that many aspects must be investigated for the retrofitting of buildings with shallow foundation in areas characterized by a medium to high level of seismic risk: (i) a key role is played by an accurate investigation of the soil; taking into account the specific conditions of the soil, it was possible to investigate its filtering effects; (ii) the dynamic response of the fully-coupled soil–structure system deviates from the free field-site response analysis; (iii) the results reveal the importance of considering the soil nonlinearity in seismic soil–structure interaction problems.
‘I Don’t Want to Be Other. I Want to Be Normal’: Mental Boundaries and the Polish Experience in the UK in Agnieszka Dale’s Fox Season and Other Short Stories
Authors: Andrés-Cuevas Isabel María
Published: April 2024

Borders and frontiers are often problematized in Agnieszka Dale’s Fox Season and Other Short Stories (2017), where mental borders seem to be more divisive than spatial boundaries. Many of these narratives feature Polish immigrants in Britain who struggle with their displaced condition in various ways. As some of the stories in the collection reveal, the scenario of post-Brexit Britain compromises conviviality amongst different groups, including the Polish community. Special attention is placed upon how several narratives in the volume underscore the prevalence in British society of Polish stereotypes as the crystallisation of the still widespread animosity against non-Europeans. Homi Bhabha’s notions regarding the formation and dynamics of stereotypes will be helpful in understanding the mechanisms beneath such constructions. Likewise, some of the major tenets of social theory, as well as Edward Said’s notion of ‘Orientalism’, will contribute to shedding light upon this resentment towards the Polish minority, occasionally adopted too by already established immigrants against their former compatriots. This article will ultimately intend to draw attention to the cautionary nature of Dale’s collection as a call for harmony and the appreciation of difference among nations, thus preventing the gloomy perspectives the dystopian futures of some of these stories forecast upon Europe.
Inappropriate Use of Emergency Services from the Perspective of Primary Care Underutilization in a Local Romanian Context: A Cross-Sectional Study
Authors: Lăcătuș Anca Maria , Atudorei Ioana Anisa , Neculau Andrea Elena , Isop Laura Mihaela , Vecerdi Cristina Agnes , Rogozea Liliana , Văcaru Mihai
Published: April 2024

Background: The underutilization of primary care services is a possible factor influencing inappropriate emergency service presentations. The objective of this study was to evaluate the proportion and characteristics of patients inappropriately accessing emergency room services from the perspective of primary care underutilization. Methods: This cross-sectional study included patients who visited the emergency room of a County Hospital, initially triaged with green, blue, or white codes, during a 2-week period in May 2017. Two primary care physicians performed a structured analysis to correlate the initial diagnosis in the emergency room with the final diagnosis to establish whether the patient’s medical complaints could have been resolved in primary care. Results: A total of 1269 adult patients were included in this study. In total, the medical problems of 71.7% of patients could have been resolved by a primary care physician using clinical skills, extended resources, or other ambulatory care and out-of-hours services. Conclusions: Low awareness of out-of-hours centers and a lack of resources for delivering more complex services in primary care can lead to inappropriate presentations to the emergency services. Future research on this topic needs to be conducted at the national level.
The AnUFGT1 Is Involved in the Anthurium ‘Alabama’ Anthocyanidin Deficiency
Authors: Li Zhiying , Wang Jiabin , Gao Yu , Jing Yonglin , Li Junguo , Xu Li
Published: April 2024

Anthurium is the second largest tropical flower crop in the world. The international market has urgent demand for anthurium varieties with different spathe colors, which mainly arises from the types and contents of anthocyanin. The flavonoid 3-O-glycosyltransferase (UF3GT) gene is the key enzyme involved in promoting anthocyanin accumulation through glycosylation downstream of the anthocyanin synthesis pathway (ASP). Abnormal functioning of UFGT usually results in a reduction in or loss of anthocyanins. The aim of this study was to reveal the role of one anthurium UFGT gene (AnUFGT1) in ‘Xueyu’ (X), an anthocyanin-deficient mutant of ‘Alabama’. Metabolome analysis was used to analyze the metabolic products in the ASP to determine the possible key link of the anthocyanin deletion mutation. Agrobacterium-mediated transformation of Arabidopsis UFGT functionally deficient mutant (ufgt) and ‘X’ validated the function of AnUFGT1. The results of comparative metabolome analysis of ‘X’ and ‘Alabama’ showed that there was no significant difference in product levels upstream of ASP. The expression levels of AnUFGT1 were significantly greater in ‘Alabama’ than in ‘X’. The overexpression of AnUFGT1 in ufgt significantly increased its anthocyanin contents. The overexpression of AnUFGT1 in ‘X’, mediated by a new injection method, can only promote the synthesis of trace anthocyanins. These results showed that AnUFGT1 could fully compensate the phenotype of ufgt, but only partially compensate the anthocyanidin-deficient phenotype of anthurium mutant X. This difference suggested that anthocyanin-deletion mutations in anthurium ‘X’ are associated with AnUFGT1, but AnUFGT1 is not the only factor. There should be other factors interacting with AnUFGT1 that cause anthocyanin deficiency.
Influence of Foliar Treatment with Suspensions Rich in Trichoderma Chlamydospores on Momordica charantia Physiology, Yield, and Quality
Authors: Bala Ioana-Alexandra , Șesan Tatiana Eugenia , Oancea Anca , Craciunescu Oana , Ghiurea Marius , Răut Iuliana , Trică Bogdan , Nicolae Cristian-Andi , Constantinescu-Aruxandei Diana , Oancea Florin
Published: April 2024

Several strategies promote phyllosphere colonization by soil-born Trichoderma plant-beneficial strains. One of these strategies is foliar spraying with suspensions containing large amounts of chlamydospores—spores with thick cell wall structures that make them highly resistant to harsh environmental conditions. Trichoderma biomass was produced by cultivation on a cornmeal medium and compared with the biomass produced on potato dextrose broth by microscopic and thermogravimetric analyses. The analyses revealed increased chlamydospore content and thermostability in the fungal biomass produced on the corn meal medium. The Trichoderma suspension rich in chlamydospores was sprayed on bitter gourd (Momordica charantia) leaves at two inoculant concentrations, 106 and 108 ufc/mL. The effect of these treatments on the plant physiological parameters, leaf photosynthetic pigments, polyphenol and flavonoid contents, antioxidant activities of the leaves and fruits, and yield was compared to the control (plants sprayed with water) and to the experimental treatment involving spraying with 108 ufc/mL of propagules produced in potato dextrose broth. The effect of chlamydospore-rich suspensions on plant physiological parameters was more pronounced and long-lasting compared with the other treatments. The treatment with chlamydospore-rich suspension enhanced the accumulation of polyphenols and flavonoids in the leaves (by 17% and 50%, respectively) and fruits (by 18% and 31%, respectively) and increased the antioxidant activity. The Trichoderma treatment increased the yield by +25.33–53.07%. The application of the foliar treatment with Trichoderma suspensions did not modify the cytocompatibility of the extracts from the fruits determined on the L929 cells.
Impact of Air Pollution and Allergic Status on Health-Related Quality of Life among University Students in Northern Thailand
Authors: Pisithkul Tipanan , Pisithkul Tippapha , Lao-Araya Mongkol
Published: April 2024

Background: Global awareness of ambient air pollution has heightened due to its detrimental impact on health, particularly in regions with elevated PM2.5 levels. Chiang Mai has emerged as an area experiencing the highest PM2.5 levels in Thailand. Objectives: to examine the prevalence of respiratory allergies and assess the impact of air pollution on the health-related quality of life (QoL) among university students in Chiang Mai. Methods: Chiang Mai University (CMU) and Maejo University (MJU) students were recruited. The Global Asthma Network (GAN) questionnaire screened for respiratory allergies (RAs). The disease-specific QoL questionnaire (Rcq-36) was administered twice during low-PM2.5 and high-PM2.5 seasons to evaluate air pollution’s impact on health-related QoL. Those showing potential RAs underwent a skin prick test (SPT) to investigate allergic sensitization. Results: Out of 406 participants, 131 (32%) reported respiratory allergies. Among those undergoing SPT, a high rate (82.54%) had positive results. Across both universities, students reported significantly lower QoL in multiple domains, particularly respiratory, eye, sleep, and emotional well-being, during the high-PM2.5 season. This aligned with their poorer self-reported health on a visual analog scale (VAS; p-value < 0.01). PM2.5 levels significantly impacted social functioning for CMU students (p-value = 0.001) and role limitations for MJU students (p-value < 0.001). Notably, participants without respiratory allergies (non-RAs) were more significantly affected by PM2.5 than RA participants in almost all parameters, despite experiencing fewer baseline symptoms. Conclusions: Respiratory allergies, particularly allergic rhinitis, are prevalent among university students in Chiang Mai. This study underscores the substantial negative impact of ambient air pollution on QoL for both allergic and non-allergic students.
Poor Self-Rated Sleep Quality and Quantity Associated with Poor Oral Health-Related Quality of Life among Indigenous Australian Adults
Authors: Ju Xiangqun , Hedges Joanne , Sethi Sneha , Jamieson Lisa M.
Published: April 2024

Background: Indigenous Australians score worse on both sleep and oral health. This study aimed to evaluate sleep quality and quantity associated with oral health-related quality of life (OHRQoL) among Indigenous Australian adults. Methods: A cross-sectional study involving 728 Indigenous Australian adults aged 18+ years was conducted. Exposure variables were sleep quality and quantity. The primary outcome variable was Oral Health Impact Profile-14 (OHIP14), which has been used to assess OHRQoL. Multivariable log–Poisson regression models were applied to estimate the mean ratios (MRs) for mean OHIP14 scores. Results: The average OHIP14 score was 14.9, and the average amount of sleep was 6.8 h/night. After adjusting for all covariates, self-rated very bad sleep quality was associated with 2.2 times (MR = 2.17, 95% CI: 1.97–2.37) higher OHIP14 scores than those who rated their sleep quality as very good. Participants who self-reported sleeping 7–8 h/night had 0.9 times (MR = 0.89, 95%CI: 0.83–0.95) lower OHIP14 scores than those sleeping more than 8 h. Conclusions: The average number of sleep hours for Indigenous participants were lower than recommended (7–8 h/night). Our findings indicate that poor sleep quality and quantity, and oral health-related behaviours associated with sleep deprivation were positively associated with poor oral health related quality of life among Indigenous Australian adults.
Balancing Flood Control and Economic Development in Flood Detention Areas of the Yangtze River Basin
Authors: Liao Siyuan , Wang Chao , Ji Renke , Zhang Xiang , Wang Zhifei , Wang Wei , Chen Nengcheng
Published: April 2024

Serving as a crucial part of the Yangtze River Basin (YRB)’s flood control system, Flood Detention Areas (FDAs) are vital in mitigating large-scale floods. Urbanization has led to the development of urban FDAs, but significant losses could ensue if these FDAs are activated. With improved reservoirs and embankments, flood pressure in the middle reaches has lessened, posing challenges in balancing flood control and economic benefits. This paper presents a comparative analysis of land use, GDP, and population in FDAs and adjacent cities, enhancing our understanding of their disparities and interrelations. Using the Analytic Hierarchy Process (AHP)–Entropy Weight Method (EW)–Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS) comprehensive evaluation method, we assess changes in flood control and economic values in FDAs. The results show a conflict between flood control and economic policies in FDAs, highlighting their underestimated economic potential, especially in urban areas. This study identifies differences in economic development across FDAs and a strong correlation between flood control value and inundation rates. Based on evaluations and simulations of the 1954 flood, we provide recommendations for the FDAs’ construction plan, which serves the development and flood management of the YRB and offer insights for similar assessments elsewhere.
Functional Identification of HhUGT74AG11—A Key Glycosyltransferase Involved in Biosynthesis of Oleanane-Type Saponins in Hedera helix
Authors: Yu Han , Zhou Jun , Zhang Jing , He Xinyi , Peng Siqing , Ling Hao , Dong Zhuang , Lu Xiangyang , Tian Yun , Guan Guiping , Tang Qi , Zhong Xiaohong , He Yuedong
Published: April 2024

Hedera helix is a traditional medicinal plant. Its primary active ingredients are oleanane-type saponins, which have extensive pharmacological effects such as gastric mucosal protection, autophagy regulation actions, and antiviral properties. However, the glycosylation-modifying enzymes responsible for catalyzing oleanane-type saponin biosynthesis remain unidentified. Through transcriptome, cluster analysis, and PSPG structural domain, this study preliminarily screened four candidate UDP-glycosyltransferases (UGTs), including Unigene26859, Unigene31717, CL11391.Contig2, and CL144.Contig9. In in vitro enzymatic reactions, it has been observed that Unigene26859 (HhUGT74AG11) has the ability to facilitate the conversion of oleanolic acid, resulting in the production of oleanolic acid 28-O-glucopyranosyl ester. Moreover, HhUGT74AG11 exhibits extensive substrate hybridity and specific stereoselectivity and can transfer glycosyl donors to the C-28 site of various oleanane-type triterpenoids (hederagenin and calenduloside E) and the C-7 site of flavonoids (tectorigenin). Cluster analysis found that HhUGT74AG11 is clustered together with functionally identified genes AeUGT74AG6, CaUGT74AG2, and PgUGT74AE2, further verifying the possible reason for HhUGT74AG11 catalyzing substrate generalization. In this study, a novel glycosyltransferase, HhUGT74AG11, was characterized that plays a role in oleanane-type saponins biosynthesis in H. helix, providing a theoretical basis for the production of rare and valuable triterpenoid saponins.
The Importance of Intra-Islet Communication in the Function and Plasticity of the Islets of Langerhans during Health and Diabetes
Authors: Hill Thomas G. , Hill David J.
Published: April 2024

Islets of Langerhans are anatomically dispersed within the pancreas and exhibit regulatory coordination between islets in response to nutritional and inflammatory stimuli. However, within individual islets, there is also multi-faceted coordination of function between individual beta-cells, and between beta-cells and other endocrine and vascular cell types. This is mediated partly through circulatory feedback of the major secreted hormones, insulin and glucagon, but also by autocrine and paracrine actions within the islet by a range of other secreted products, including somatostatin, urocortin 3, serotonin, glucagon-like peptide-1, acetylcholine, and ghrelin. Their availability can be modulated within the islet by pericyte-mediated regulation of microvascular blood flow. Within the islet, both endocrine progenitor cells and the ability of endocrine cells to trans-differentiate between phenotypes can alter endocrine cell mass to adapt to changed metabolic circumstances, regulated by the within-islet trophic environment. Optimal islet function is precariously balanced due to the high metabolic rate required by beta-cells to synthesize and secrete insulin, and they are susceptible to oxidative and endoplasmic reticular stress in the face of high metabolic demand. Resulting changes in paracrine dynamics within the islets can contribute to the emergence of Types 1, 2 and gestational diabetes.
Toxicity Mechanisms of Gadolinium and Gadolinium-Based Contrast Agents—A Review
Authors: Coimbra Susana , Rocha Susana , Sousa Nícia Reis , Catarino Cristina , Belo Luís , Bronze-da-Rocha Elsa , Valente Maria João , Santos-Silva Alice
Published: April 2024

Gadolinium-based contrast agents (GBCAs) have been used for more than 30 years to improve magnetic resonance imaging, a crucial tool for medical diagnosis and treatment monitoring across multiple clinical settings. Studies have shown that exposure to GBCAs is associated with gadolinium release and tissue deposition that may cause short- and long-term toxicity in several organs, including the kidney, the main excretion organ of most GBCAs. Considering the increasing prevalence of chronic kidney disease worldwide and that most of the complications following GBCA exposure are associated with renal dysfunction, the mechanisms underlying GBCA toxicity, especially renal toxicity, are particularly important. A better understanding of the gadolinium mechanisms of toxicity may contribute to clarify the safety and/or potential risks associated with the use of GBCAs. In this work, a review of the recent literature concerning gadolinium and GBCA mechanisms of toxicity was performed.
Human Vault RNAs: Exploring Their Potential Role in Cellular Metabolism
Authors: Taube Magdalena , Lisiak Natalia , Totoń Ewa , Rubiś Błażej
Published: April 2024

Non-coding RNAs have been described as crucial regulators of gene expression and guards of cellular homeostasis. Some recent papers focused on vault RNAs, one of the classes of non-coding RNA, and their role in cell proliferation, tumorigenesis, apoptosis, cancer response to therapy, and autophagy, which makes them potential therapy targets in oncology. In the human genome, four vault RNA paralogues can be distinguished. They are associated with vault complexes, considered the largest ribonucleoprotein complexes. The protein part of these complexes consists of a major vault protein (MVP) and two minor vault proteins (vPARP and TEP1). The name of the complex, as well as vault RNA, comes from the hollow barrel-shaped structure that resembles a vault. Their sequence and structure are highly evolutionarily conserved and show many similarities in comparison with different species, but vault RNAs have various roles. Vaults were discovered in 1986, and their functions remained unclear for many years. Although not much is known about their contribution to cell metabolism, it has become clear that vault RNAs are involved in various processes and pathways associated with cancer progression and modulating cell functioning in normal and pathological stages. In this review, we discuss known functions of human vault RNAs in the context of cellular metabolism, emphasizing processes related to cancer and cancer therapy efficacy.
Investigating the Effects of Diet-Induced Prediabetes on Skeletal Muscle Strength in Male Sprague Dawley Rats
Authors: Dlamini Mandlakazi , Khathi Andile
Published: April 2024

Type 2 diabetes mellitus, a condition preceded by prediabetes, is documented to compromise skeletal muscle health, consequently affecting skeletal muscle structure, strength, and glucose homeostasis. A disturbance in skeletal muscle functional capacity has been demonstrated to induce insulin resistance and hyperglycemia. However, the modifications in skeletal muscle function in the prediabetic state are not well elucidated. Hence, this study investigated the effects of diet-induced prediabetes on skeletal muscle strength in a prediabetic model. Male Sprague Dawley rats were randomly assigned to one of the two groups (n = 6 per group; six prediabetic (PD) and six non-pre-diabetic (NPD)). The PD group (n = 6) was induced with prediabetes for 20 weeks. The diet that was used to induce prediabetes consisted of fats (30% Kcal/g), proteins (15% Kcal/g), and carbohydrates (55% Kcal/g). In addition to the diet, the experimental animals (n = 6) were supplied with drinking water that was supplemented with 15% fructose. The control group (n = 6) was allowed access to normal rat chow, consisting of 35% carbohydrates, 30% protein, 15% fats, and 20% other components, as well as ordinary tap water. At the end of week 20, the experimental animals were diagnosed with prediabetes using the American Diabetes Association (ADA) prediabetes impaired fasting blood glucose criteria (5.6–6.9 mmol/L). Upon prediabetes diagnosis, the animals were subjected to a four-limb grip strength test to assess skeletal muscle strength at week 20. After the grip strength test was conducted, the animals were euthanized for blood and tissue collection to analyze glycated hemoglobin (HbA1c), plasma insulin, and insulin resistance using the homeostatic model of insulin resistance (HOMA-IR) index and malondialdehyde (MDA) concentration. Correlation analysis was performed to examine the associations of skeletal muscle strength with HOMA-IR, plasma glucose, HbA1c, and MDA concentration. The results demonstrated increased HbA1c, FBG, insulin, HOMA-IR, and MDA concentrations in the PD group compared to the NPD group. Grip strength was reduced in the PD group compared to the NPD group. Grip strength was negatively correlated with HbA1c, plasma glucose, HOMA-IR, and MDA concentration in the PD group. These observations suggest that diet-induced prediabetes compromises muscle function, which may contribute to increased levels of sedentary behavior during prediabetes progression, and this may contribute to the development of hyperglycemia in T2DM.