bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Gluten Withdrawal on Health-Related Quality of Life in Celiac Subjects: An Observational Case-Control Study
Authors: Tontini Gian Eugenio , Rondonotti Emanuele , Saladino Valeria , Saibeni Simone , de Franchis Roberto , Vecchi Maurizio
Published: June 2010

Aims: To evaluate health-related quality of life (HRQoL) in celiac disease (CD) patients at the time of diagnosis and during a gluten-free diet (GFD). Patients and Methods: We enrolled 43 adult CD patients (18 with a typical and 15 with an atypical clinical presentation, and 10 with dermatitis herpetiformis, DH) and 86 age- and sex-matched healthy controls. We administered the Short Form 36 Health Survey (SF-36) questionnaire at diagnosis and after 1, 12 and 24 months of a GFD. Results: At the time of diagnosis CD patients showed significantly lower SF-36 scores than controls; this figure was observed in women but not in men. At baseline, both typical and atypical CD patients had lower SF-36 scores than controls, while DH patients showed a SF-36 profile comparable to that of controls. During a GFD the SF-36 scores improved continuously in CD patients and in the female subgroup, becoming similar to those of matched controls at 1-year follow-up. After gluten withdrawal typical and atypical CD patients improved their SF-36 scores and reached values comparable to those of controls. Conclusions: At diagnosis, CD patients perceived a poor HRQoL; this figure appears to be mostly associated with female gender. In all subgroups of CD patients with a low HRQoL at diagnosis, the GFD allowed progressive restoration of HRQoL perception.
Reduction of Free Immunoglobulin Light Chains Using Adsorption Properties of Hemodiafiltration with Endogenous Reinfusion
Authors: Testa Angelo , Dejoie Thomas , Lecarrer Didier , Wratten Marylou , Sereni Luisa , Renaux Jean Louis
Published: June 2010

In this work we investigated the acute effects of hemodiafiltration with endogenous reinfusion (HFR therapy) on the removal of free immunoglobulin light chains (FIgLCs), which may be considered members of the family of uremic toxins. In two groups of patients – group 1 (polyclonal FIgLCs production) and group 2 (monoclonal plasma cell proliferative disorders), we analyzed the pre- and postdialysis levels of ĸ- and λ-chains. In group 1 we observed a significant reduction of FIgLCs (p < 0.01). A similar trend was found in patients of group 2 only for ĸ-chains. The FIgLCs removal ratio was significantly higher for ĸ- than λ-chains in the two patient groups. In vitro data showed affinity of macroporous resin to binding FIgLCs. Our results show that the HFR therapy could be effective in removing FIgLCs, particularly ĸ-chains in dialysis patients with polyclonal and monoclonal FIgLCs production.
Analysis of Stereotactic Accuracy of the Cosman-Robert-Wells Frame and Nexframe Frameless Systems in Deep Brain Stimulation Surgery
Authors: Kelman Craig , Ramakrishnan V. , Davies Alex , Holloway Kathryn
Published: June 2010

Introduction: The primary goal of stereotactic systems in deep brain stimulation (DBS) surgery is accurate delivery of a DBS lead to a target identified on imaging. Thus, it is critical to understand the accuracy of the stereotactic systems and the factors which may be associated with a decrease in accuracy. Methods: Ninety patients underwent microelectrode recording-guided placement of 139 DBS leads by a single surgeon using the Cosman-Roberts-Wells (CRW) frame (n = 70) or a frameless skull-mounted trajectory guide (Nexframe; n = 69). The final DBS location was identified on a postoperative CT fused to the preoperative CT and MRI scans. The difference between this final location and the expected location was calculated. Results: The vector error was 2.65 mm (standard error, 0.22) for the frame and 2.78 mm (standard error, 0.25) for the frameless methods (p = 0.69). There was a gradual decline in error for both systems over time, as the vector error of the last 20 implants was 1.99 for the CRW frame and 2.04 for the Nexframe (p = 0.86). Conclusions: This study shows that the CRW frame and Nexframe frameless systems have equivalent accuracy. Furthermore, the accuracy of both techniques improved over time, from 3 mm initially to 2 mm with current techniques.
Competing Oscillators in Cardiac Pacemaking
Authors: Noble Denis , Noble Penelope J. , Fink Martin
Published: June 2010

Abstract
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Interaction between a membrane oscillator generated by voltage-dependent ion channels and an intracellular calcium signal oscillator was present in the earliest models (1984 to 1985) using representations of the sarcoplasmic reticulum. Oscillatory release of calcium is inherent in the calcium-induced calcium release process. Those historical results fully support the synthesis proposed in the articles in this review series. The oscillator mechanisms do not primarily compete with each; they entrain each other. However, there is some asymmetry: the membrane oscillator can continue indefinitely in the absence of the calcium oscillator. The reverse seems to be true only in pathological conditions. Studies from tissue-level work and on the development of the heart also provide valuable insights into the integrative action of the cardiac pacemaker.
Prospects of the WSR-88D Radar for Cloud Studies
Authors: Melnikov Valery M. , Zrnić Dusan S. , Doviak Richard J. , Chilson Phillip B. , Mechem David B. , Kogan Yefim L.
Published: June 2010

Sounding of nonprecipitating clouds with the 10-cm wavelength Weather Surveillance Radar-1988 Doppler (WSR-88D) is discussed. Readily available enhancements to signal processing and volume coverage patterns of the WSR-88D allow observations of a variety of clouds with reflectivities as low as −25 dBZ (at a range of 10 km). The high sensitivity of the WSR-88D, its wide velocity and unambiguous range intervals, and the absence of attenuation allow accurate measurements of the reflectivity factor, Doppler velocity, and spectrum width fields in clouds to ranges of about 50 km. Fields of polarimetric variables in clouds, observed with a research polarimetric WSR-88D, demonstrate an abundance of information and help to resolve Bragg and particulate scatter. The scanning, Doppler, and polarimetric capabilities of the WSR-88D allow real-time, three-dimensional mapping of cloud processes, such as transformations of hydrometeors between liquid and ice phases. The presence of ice particles is revealed by high differential reflectivities and the lack of correlation between reflectivity and differential reflectivity in clouds in contrast to that found for rain. Pockets of high differential reflectivities are frequently observed in clouds; maximal values of differential reflectivity exceed 8 dB, far above the level observed in rain. The establishment of the WSR-88D network consisting of 157 polarimetric radars can be used to collect cloud data at any radar site, making the network a potentially powerful tool for climatic studies.
A Simple Technique for Creating Regional Composites of Sea Surface Temperature from MODIS for Use in Operational Mesoscale NWP
Authors: Knievel Jason C. , Rife Daran L. , Grim Joseph A. , Hahmann Andrea N. , Hacker Joshua P. , Ge Ming , Fisher Henry H.
Published: June 2010

This paper describes a simple technique for creating regional, high-resolution, daytime and nighttime composites of sea surface temperature (SST) for use in operational numerical weather prediction (NWP). The composites are based on observations from NASA’s Moderate Resolution Imaging Spectroradiometer (MODIS) aboard Aqua and Terra. The data used typically are available nearly in real time, are applicable anywhere on the globe, and are capable of roughly representing the diurnal cycle in SST. The composites’ resolution is much higher than that of many other standard SST products used for operational NWP, including the low- and high-resolution Real-Time Global (RTG) analyses. The difference in resolution is key because several studies have shown that highly resolved SSTs are important for driving the air–sea interactions that shape patterns of static stability, vertical and horizontal wind shear, and divergence in the planetary boundary layer. The MODIS-based composites are compared to in situ observations from buoys and other platforms operated by the National Data Buoy Center (NDBC) off the coasts of New England, the mid-Atlantic, and Florida. Mean differences, mean absolute differences, and root-mean-square differences between the composites and the NDBC observations are all within tenths of a degree of those calculated between RTG analyses and the NDBC observations. This is true whether or not one accounts for the mean offset between the skin temperatures of the MODIS dataset and the bulk temperatures of the NDBC observations and RTG analyses. Near the coast, the MODIS-based composites tend to agree more with NDBC observations than do the RTG analyses. The opposite is true away from the coast. All of these differences in point-wise comparisons among the SST datasets are small compared to the ±1.0°C accuracy of the NDBC SST sensors. Because skin-temperature variations from land to water so strongly affect the development and life cycle of the sea breeze, this phenomenon was chosen for demonstrating the use of the MODIS-based composite in an NWP model. A simulated sea breeze in the vicinity of New York City and Long Island shows a small, net, but far from universal improvement when MODIS-based composites are used in place of RTG analyses. The timing of the sea breeze’s arrival is more accurate at some stations, and the near-surface temperature, wind, and humidity within the breeze are more realistic.
The Impact of Wind Stress Feedback on the Stability of the Atlantic Meridional Overturning Circulation
Authors: Arzel Olivier , England Matthew H. , Saenko Oleg A.
Published: June 2010

Recent results based on models using prescribed surface wind stress forcing have suggested that the net freshwater transport Σ by the Atlantic meridional overturning circulation (MOC) into the Atlantic basin is a good indicator of the multiple-equilibria regime. By means of a coupled climate model of intermediate complexity, this study shows that this scalar Σ cannot capture the connection between the properties of the steady state and the impact of the wind stress feedback on the evolution of perturbations. This implies that, when interpreting the observed value of Σ, the position of the present-day climate is systematically biased toward the multiple-equilibria regime. The results show, however, that the stabilizing influence of the wind stress feedback on the MOC is restricted to a narrow window of freshwater fluxes, located in the vicinity of the state characterized by a zero freshwater flux divergence over the Atlantic basin. If the position of the present-day climate is farther away from this state, then wind stress feedbacks are unable to exert a persistent effect on the modern MOC. This is because the stabilizing influence of the shallow reverse cell situated south of the equator during the off state rapidly dominates over the destabilizing influence of the wind stress feedback when the freshwater forcing gets stronger. Under glacial climate conditions by contrast, a weaker sensitivity with an opposite effect is found. This is ultimately due to the relatively large sea ice extent of the glacial climate, which implies that, during the off state, the horizontal redistribution of fresh waters by the subpolar gyre does not favor the development of a thermally direct MOC as opposed to the modern case.
Revisiting the Influence of the Quasi-Biennial Oscillation on Tropical Cyclone Activity
Authors: Camargo Suzana J. , Sobel Adam H.
Published: June 2010

The statistical relationship between the quasi-biennial oscillation (QBO) and tropical cyclone (TC) activity is explored, with a focus on the North Atlantic. Although there is a statistically significant relationship between the QBO and TCs in the Atlantic from the 1950s to the 1980s, as found by previous studies, that relationship is no longer present in later years. Several possibilities for this change are explored, including the interaction with ENSO, volcanoes, QBO decadal variability, and interactions with solar forcing. None provides a completely satisfying explanation. In the other basins, the relationship is weaker than in the Atlantic, even in the early record.
Everyday Sensegiving: A Closer Look at Successful Plant Managers
Authors: Smith Anne D. , Ashmos Plowman Donde , Duchon Dennis
Published: June 2010

The authors conducted in-depth interviews and on-site visits with successful plant managers to understand similarities in their management approaches. Across 11 different plants, representing nine different industries, the authors found each plant manager actively engaged in shaping how employees viewed the organization and its values through what the authors call “everyday sensegiving.” From themes inductively identified from the interviews and on-site visits, four central values—“Here, we value people, we value openness, we value being positive, and we value being part of a larger community” were identified. In this article, the authors link everyday sensegiving of these middle managers and extend the findings of the study to consider linkages to organizational performance and change efforts.
Social Cognition and Its Relation to Psychosocial Adjustment in Children With Nonverbal Learning Disabilities
Authors: Galway Tanya M. , Metsala Jamie L.
Published: June 2010

The current study examined social cognitive skills in children with nonverbal learning disabilities (NLD) compared to normally achieving (NA) children. The relation between social cognitive skills and psychosocial adjustment was also investigated. There were no group differences on children’s ability to represent orally presented social vignettes. Children with NLD were less able to recognize self-generated competent/assertive responses as the best solutions to a problem, expected fewer positive and more negative outcomes for examiner-provided competent responses, and showed a trend toward more frequently judging a story character as being mean. A social problem solving factor predicted unique variance in psychosocial adjustment beyond variance accounted for by nonverbal intelligence and nonverbal social cue interpretation. Results are discussed within a framework for which social cognitive deficits more broadly defined than encoding and interpreting nonverbal social cues contribute to the psychosocial adjustment problems of children with NLD.
Automatic Effects of Alcohol and Aggressive Cues on Aggressive Thoughts and Behaviors
Authors: Subra Baptiste , Muller Dominique , Bègue Laurent , Bushman Brad J. , Delmas Florian
Published: June 2010

Numerous studies have shown that alcohol increases aggression. In this article it is proposed that the link between alcohol and aggression is so strong that mere exposure to alcohol-related cues will automatically activate aggressive thoughts and behaviors. Two experiments tested this automaticity theory of alcohol-related aggression. In Experiment 1, participants exposed to alcohol- or weapon-related primes made faster lexical decisions about aggression-related words than did participants exposed to neutral primes. In Experiment 2, participants exposed to alcohol- or aggression-related subliminal primes were more aggressive toward the experimenter than were participants exposed to neutral subliminal primes. In both experiments, the effects of alcohol-related cues were as strong as the effect of aggression-related cues on aggressive thoughts and behaviors. People do not need to drink a drop of alcohol to become aggressive; exposure to alcohol cues is enough to automatically increase aggression.
Evaluation of Diuron (3-[3,4-dichlorophenyl]-1,1-dimethyl urea) in a Two-stage Mouse Skin Carcinogenesis Assay
Authors: Ferrucio Bianca , Franchi Carla Adriene da Silva , Boldrin Natália Ferreira , Oliveira Maria Luiza Cotrim Sartor de , Camargo João Lauro Viana de
Published: June 2010

Diuron (3-[3,4-dichlorophenyl]-1,1-dimethyl urea) is an herbicide with carcinogenic activity in rats and mice, which have developed respectively urothelial and mammary gland tumors in long-term studies. Accordingly, diuron has been categorized as a “likely human carcinogen” by the U.S. Environmental Protection Agency. Although the carcinogenesis-initiating activity of diuron has been reported in an early initiation-promotion mouse skin study, its genotoxic potential has been disputed. It is necessary to clarify the mode of action through which it has caused rodent neoplasia and verify its relevance to humans. Herein, two experiments were developed to verify the initiating and promoting potentials of diuron in a twenty-three- and a twenty-one-week–long mouse skin carcinogenesis protocol. In one, dimethylsulfoxide (DMSO) was the solvent for the herbicide; in the other, acetone was the alternative solvent in order to verify whether DMSO had inhibitory influence on a potential cutaneous carcinogenic activity. The adopted schedule for the tumor-promoting agent 12-O-tetradecanoylphorbol 13-acetate (TPA) resulted in skin ulcers, which demonstrates the need for careful selection of TPA dose levels and frequency of application in this model. In both studies, diuron did not exert any influence on the skin carcinogenesis process, in contrast with results already reported in the literature.
Design and field experimentation of a prototype Lunar prospector
Authors: Wettergreen David , Moreland Scott , Skonieczny Krzysztof , Jonak Dominic , Kohanbash David , Teza James
Published: June 2010

Scarab is a prototype rover for Lunar missions to survey resources in polar craters. It is designed as a prospector that would use a deep coring drill and apply soil analysis instruments to measure the abundance of elements of hydrogen and oxygen and other volatiles including water. Scarab’s chassis can adjust the wheelbase and height to stabilize its drill in contact with the ground and can also adjust posture to better ascend and descend steep slopes. This enables unique control of posture when moving and introduces new planning issues. Scarab has undergone field testing at Lunar-analog sites in Washington and Hawaii in an effort to quantify and validate its mobility and navigation capabilities. We report on results of the experiments in slope ascent and descent and in autonomous kilometer-distance navigation in darkness.
Online and Incremental Appearance-based SLAM in Highly Dynamic Environments
Authors: Kawewong Aram , Tongprasit Noppharit , Tangruamsub Sirinart , Hasegawa Osamu
Published: June 2010

In this paper we present a novel method for online and incremental appearance-based localization and mapping in a highly dynamic environment. Using position-invariant robust features (PIRFs), the method can achieve a high rate of recall with 100% precision. It can handle both strong perceptual aliasing and dynamic changes of places efficiently. Its performance also extends beyond conventional images; it is applicable to omnidirectional images for which the major portions of scenes are similar for most places. The proposed PIRF-based Navigation method named PIRF-Nav is evaluated by testing it on two standard datasets in a similar manner as in FAB-MAP and on an additional omnidirectional image dataset that we collected. This extra dataset was collected on 2 days with different specific events, i.e. an open-campus event, to present challenges related to illumination variance and strong dynamic changes, and to test assessment of dynamic scene changes. Results show that PIRF-Nav outperforms FAB-MAP; PIRF-Nav at precision-1 yields a recall rate about twice as high (approximately 80% increase) than that of FAB-MAP. Its computation time is sufficiently short for real-time applications. The method is fully incremental, and requires no offline process for dictionary creation. Additional testing using combined datasets proves that PIRF-Nav can function over the long term and can solve the kidnapped robot problem.
DIAGNOSTIC EXERCISE
Authors: Ellis A. E. , Brown C. A. , Miller D. L.
Published: June 2010

An approximately one-and-a-half-year-old, neutered male, mixed-breed dog was presented for a chronic history of vomiting. Profuse diarrhea was also noted during examination. An exploratory laparotomy was performed, bone chips were removed from the stomach, and a raised, circular area of gastric mucosa was biopsied. Histologically, there was severe gastric cryptosporidiosis as well as numerous spiral bacteria, consistent with Helicobacter spp. Polymerase chain reaction revealed visible bands for the 18S ribosomal RNA gene for Cryptosporidium spp. The polymerase chain reaction product was sequenced and was found to be most similar to Cryptosporidium muris. Both the gastric location and the species of Cryptosporidium are unusual in a dog.
Study on the Infectivity of Equine Herpesvirus 9 (EHV-9) by Different Routes of Inoculation in Hamsters
Authors: El-Habashi N. , Murakami M. , El-Nahass E. , Hibi D. , Sakai H. , Fukushi H. , Sasseville V. , Yanai T.
Published: June 2010

The infectivity and pathology of equine herpesvirus 9 (EHV-9), a new neurotropic equine herpesvirus isolated from gazelles, was studied in hamsters experimentally infected via nasal, ocular, oral, intravenous (IV), or peritoneal routes. Clinically, all animals inoculated by the nasal route and ~25% inoculated by the oral and peritoneal routes showed neurological signs on days 3, 6, and 9 postinoculation (PI), respectively. Neurological signs were not observed in animals administered EHV-9 by the IV and ocular routes. With the exception of animals administered EHV-9 by the IV route, all infected animals had lymphocytic meningoencephalitis. Although there were a number of differences in the severity and distribution of the lesions depending on the route of inoculation, the basic features of lymphocytic meningoencephalitis caused by EHV-9 were common. Lesions consisted of neuronal necrosis, perivascular aggregates of lymphocytes, plasma cells, and neutrophils, gliosis, intranuclear inclusion bodies, and diffuse lymphocytic infiltrates in the meninges. Viral antigen was detected in degenerated neurons in infected animals inoculated by the nasal, ocular, oral, and peritoneal routes. The distribution of EHV-9 antigen was somewhat dependent on inoculation route. There were no microscopic abnormalities or viral antigen in animals treated by the IV route. This study provides new data about experimental EHV-9 infection in hamsters through routes other than the IV route. These results suggest that in the animals infected by the oral, ocular, and peritoneal routes, EHV-9 might travel to the brain through nerves, other than by the olfactory route, after initial propagation at the site of viral entry.
Modeling the Politics of Punishment: A Contextual Analysis of Racial Disparity in Drug Sentencing
Authors: Helms Ronald , Costanza S.E.
Published: June 2010

This study uses Tobit to assess contextual punishment determinants for a large sample of felony drug cases that reached final disposition in 1990. After statistically holding constant ascribed and legal variables, the authors find that punishments for African American defendants in drug-related cases varied by social and political context. African American defendants adjudicated in jurisdictions characterized by a large Black population received reduced punishments; but in jurisdictions that were characterized by strong law-and-order political support, Black defendants received longer sentences. After introducing these interactions, Blacks faced on average reduced penalties for drug crimes. Blacks were the recipients of adjusted sentencing but not in the uniformly harsh direction proposed by much of the sentencing research. In sum, the results of this research add to the growing literature documenting the political foundations of punishment patterns in the U.S. criminal courts.
Benefit effect of naloxone in benzodiazepines intoxication: Findings of a preliminary study
Authors: Solhi Hassan , Mostafazadeh Babak , Reza Khoddami Vishteh Hamid , Reza Ghezavati Ali , Shooshtarizadeh Ali
Published: June 2010

Background: Naloxone, as a low-priced and available drug, may be useful in improvement of signs and symptoms of benzodiazepines intoxication. The aim of this study was assessment of its effect on benzodiazepines poisoning. Methods: In this clinical-trial study, patients with typical signs and symptoms of benzodiazepines poisoning, who were referred to a poisoning center in Tehran in 2008, were selected. After recording of patients’ characteristics, supportive treatment was initiated and patients were randomly assigned to the case group with intravenous (IV) injection of two 0.4 mg naloxone ampules or to the control group. Their signs and symptoms were evaluated again 0.5 hour later. Each of diazepam, clonazepam and alperazolam drug group had 30 patients and lorazepam drug group had 26 patients, half of which patients in each drug group received naloxone. Results: Most of the participants were female and the mean age was 28 years. There were no significant differences between case and control groups in age, sex, time of drug consumption, tablet counts, signs and symptoms and level of consciousness at the admission time in each drug types. After naloxone injection in case groups, all signs and symptoms significantly improved in all drug types in comparison to control groups except nystagmus. In addition, level of consciousness significantly improved in case groups in all drug types except lorazepam. Conclusion: Findings of the study showed that naloxone is effective in management of benzodiazepines poisoning. However, future clinical trials with greater sample size are recommened.
Comorbidity of Asthma With ADHD
Authors: Fasmer Ole Bernt , Riise Trond , Eagan Tomas Mikal , Lund Anders , Dilsaver Steven C. , Hundal Øivind , Oedegaard Ketil J.
Published: June 2010

Objective: To assess how frequently drugs used to treat asthma and ADHD are prescribed to the same patients. Method: The authors used data from the Norwegian Prescription Database for 2006, including the total Norwegian population ( n = 4,640,219). Results: Anti-asthma drugs were prescribed to 350,894 persons (7.56 % of the population), anti-ADHD drugs to 18,481 persons (0.40 %), and both to 1,730 persons. There was a 65% increased overall risk ( OR = 1.65) of being prescribed one of the drugs given a prescription of the other. Women had a markedly higher risk than men. When data for each age group (10 years interval) and each gender were analyzed separately, the strongest associations were found for women between 20 and 49 years of age and men between 30 and 49 years of age. Conclusion: These prescription patterns suggested a marked comorbidity between asthma and ADHD.
Listening to the monkey: Class, youth and the formation of a musical habitus
Authors: Rimmer Mark
Published: June 2010

This article explores the musical lives and music-related activities of a group of young people living on a deprived housing estate in North East England. Through the use of ethnography, I uncover how central aspects of these young people’s taste for, and uses of, their preferred ‘new monkey’ music function in respect of the values inscribed in their community’s past, the problems and challenges facing them in the present, as well as their anticipations of the future. To help think through the complex and sometimes contradictory processes at work within these young musico-cultural lives, I employ and specifically adapt a key Bourdieusian concept to encouraging thinking about such issues in terms of a musical habitus. As an invitation to explore the nexus of personal, social and historical factors that conjoin music’s possibilities with the contexts and conditions of lived practices, the concept of musical habitus reveals substantial explanatory potential.
Rupture of an Abdominal Aortic Aneurysm Post EVAR: A Rare Presentation of Retroperitoneal Liposarcoma
Authors: Paravastu Sharath C.V. , Ghosh Jonathan , Farquharson Finn G. , Walker Michael G.
Published: June 2010

Endoleak is the classical cause of rupture of aneurysms previously treated by endovascular means. We report a rare case of a retroperitoneal liposarcoma (LIS) invading an abdominal aortic aneurysm (AAA), previously treated by endovascular repair (EVAR), causing rupture. Furthermore, a brief discussion of the diagnostic challenges posed by retroperitoneal tumors is presented with a closing note on their management.
Review: Contemporary practices in postcardiac arrest syndrome: the role of mild therapeutic hypothermia
Authors: Gavrielatos Gerasimos , Werner Klaus Dieter , Voridis Eftichios , Kremastinos Dimitrios T.
Published: June 2010

Out-of-hospital cardiac arrest remains a major cause of mortality and morbidity despite progress in resuscitative practices. The number of survivors with severe neurological impairment at hospital discharge is similarly dismal. Recently, much attention has been directed toward the use of mild therapeutic hypothermia in the care of comatose survivors with postcardiac arrest syndrome. Recent research suggests mild hypothermia lowers mortality and improves neurological outcome after successful treatment of cardiac arrest. The current 2005 updated guidelines of International Liaison Committee on Resuscitation and European Resuscitation Council recommend the utilization of mild induced hypothermia in postresuscitation treatment. Hypothermia induction in order to avoid the pathophysiological mechanisms of euthermia and hyperthermia and subsequent complications are briefly discussed. Cooling methods, potential side effects and questions regarding implementation of therapeutic hypothermia recommendations in every day clinical practice and future investigation are also addressed.
Possible clinical outcome measures for clinical trials in patients with multiple sclerosis
Authors: Goldman Myla D. , Motl Robert W. , Rudick Richard A.
Published: June 2010

Multiple sclerosis (MS) is a chronic inflammatory and neurodegenerative disease with both clinical and pathological heterogeneity. The complexity of the MS population has offered challenges to the measurement of MS disease progression in therapeutic trials. The current standard clinical outcome measures are relapse rate, Expanded Disability Severity Scale (EDSS), and the MS Functional Composite (MSFC). These measures each have strengths and some weakness. Two additional measures, the six-minute walk and accelerometry, show promise in augmenting current measures. MS therapeutics is a quickly advancing field which requires sensitive clinical outcome measures that can detect small changes in disability that reliably reflect long-term changes in sustained disease progression in a complex population. A single clinical outcome measure of sustained disease progression may remain elusive. Rather, an integration of current and new outcome measures may be most appropriate and utilization of different measures depending on the MS population and stage of the disease may be preferred.
Multiple system atrophy: current and future approaches to management
Authors: Flabeau Olivier , Meissner Wassilios G. , Tison François
Published: June 2010

Multiple system atrophy (MSA) is a rare neurodegenerative disorder without any effective treatment in slowing or stopping disease progression. It is characterized by poor levodopa responsive Parkinsonism, cerebellar ataxia, pyramidal signs and autonomic failure in any combination. Current therapeutic strategies are primarily based on dopamine replacement and improvement of autonomic failure. However, symptomatic management remains disappointing and no curative treatment is yet available. Recent experimental evidence has confirmed the key role of alpha-synuclein aggregation in the pathogenesis of MSA. Referring to this hypothesis, transgenic and toxic animal models have been developed to assess candidate drugs for MSA. The standardization of diagnosis criteria and assessment procedures will allow large multicentre clinical trials to be conducted. In this article we review the available symptomatic treatment, recent results of studies investigating potential neuroprotective drugs, and future approaches for the management in MSA.
Pediatric Nutrition Private Practice
Authors: Castle Jill L.
Published: June 2010

The area of private practice for the pediatric registered dietitian (RD) is an exciting and emerging field of opportunity. As the specialized nutritional concerns of children gain national emphasis, and the consumer interest for practical nutrition knowledge increases, the stage is set for the role of the pediatric RD to expand beyond the clinical, hospitalized setting to prevention and treatment and to support endeavors in the private setting. More than ever, families are seeking nutritional guidance and are in need of a reliable resource. Simultaneously, a gap is growing: patient contact time with health care professionals is decreasing while children steadily develop nutritional challenges. The pediatric RD in private practice can close this widening gap by providing nutrition services that aim to prevent and address the numerous nutritional challenges of childhood. Operating a private practice in pediatric nutrition can be a fulfilling, challenging, and creative area for pediatric RDs. Traditional one-on-one counseling, group classes and seminars, consulting, teaching, and writing are just a few examples of the myriad endeavors a pediatric RD can blend into her or his pediatric nutrition business. This article will provide an insight into the personal, practical, and business considerations for starting your own private practice in pediatric nutrition.
Technician Training Reduces Formula Preparation Errors
Authors: Dumm Marsha , Peel Lynda , Jones Amy , Hunter Crystal , Kendall-Harris Mary , Boltwood Charles , Hunt Charles , Valentine Christina
Published: June 2010

Neonatal intensive care patients are at high risk of infection because of immature immune status. Cases of illness and death have been reported as a result of formula-related problems. Powdered formulas are discouraged due to potential bacterial contamination and sterile liquid products are not available in nutrient concentrations required to support growth and development of fragile neonates. An increased number of formula errors revealed the need for training of formula room technicians. Specific training targeted areas of formula preparation that would decrease errors and improve safety and accuracy of product delivered to high-risk neonates.
Reconstitution of PTEN activity by CK2 inhibitors and interference with the PI3-K/Akt cascade counteract the antiapoptotic effect of human stromal cells in chronic lymphocytic leukemia
Authors: Shehata Medhat , Schnabl Susanne , Demirtas Dita , Hilgarth Martin , Hubmann Rainer , Ponath Elena , Badrnya Sigrun , Lehner Claudia , Hoelbl Andrea , Duechler Markus , Gaiger Alexander , Zielinski Christoph , Schwarzmeier Josef D. , Jaeger Ulrich
Published: June 2010

Evidence suggests that tumor microenvironment is critically involved in supporting survival of chronic lymphocytic leukemia (CLL) cells. However, the molecular mechanisms of this effect and the clinical significance are not fully understood. We applied a microenvironment model to explore the interaction between CLL cells and stromal cells and to elucidate the role of phosphatidylinositol 3 kinase (PI3-K)/Akt/phosphatase and tensin homolog detected on chromosome 10 (PTEN) cascade in this process and its in vivo relevance. Primary human stromal cells from bone marrow, lymph nodes, and spleen significantly inhibited spontaneous apoptosis of CLL cells. Pan–PI3-K inhibitors (LY294002, wortmannin, PI-103), isotype-specific inhibitors of p110α, p110β, p110γ, and small interfering RNA against PI3-K and Akt1 counteracted the antiapoptotic effect of the stromal cells. Induction of apoptosis was associated with a decrease in phosphatidylinositol-3,4,5-triphosphate, PI3-K–p85, and dephosphorylation of phosphatidylinositol-dependent kinase-1 (PDK-1), Akt1, and PTEN. Freshly isolated peripheral blood mononuclear cells from patients with CLL (n = 44) showed significantly higher levels of phosphorylated Akt1, PDK-1, PTEN, and CK2 than healthy persons (n = 8). CK2 inhibitors (4,5,6,7-tetrabromo-1H-benzotriazole, apigenin, and 5,6-dichloro-1-β-D-ribofuranosylbenzimidazol) decreased phosphorylation of PTEN and Akt, induced apoptosis in CLL cells, and enhanced the response to fludarabine. In conclusion, bone marrow microenvironment modulates the PI3-K/Akt/PTEN cascade and prevents apoptosis of CLL cells. Combined inhibition of PI3-K/Akt and recovery of PTEN activity may represent a novel therapeutic concept for CLL.
A semi-supervised learning approach to predict synthetic genetic interactions by combining functional and topological properties of functional gene network
Authors: You Zhu-Hong , Yin Zheng , Han Kyungsook , Huang De-Shuang , Zhou Xiaobo
Published: June 2010

Background

Genetic interaction profiles are highly informative and helpful for understanding the functional linkages between genes, and therefore have been extensively exploited for annotating gene functions and dissecting specific pathway structures. However, our understanding is rather limited to the relationship between double concurrent perturbation and various higher level phenotypic changes, e.g. those in cells, tissues or organs. Modifier screens, such as synthetic genetic arrays (SGA) can help us to understand the phenotype caused by combined gene mutations. Unfortunately, exhaustive tests on all possible combined mutations in any genome are vulnerable to combinatorial explosion and are infeasible either technically or financially. Therefore, an accurate computational approach to predict genetic interaction is highly desirable, and such methods have the potential of alleviating the bottleneck on experiment design.

Results

In this work, we introduce a computational systems biology approach for the accurate prediction of pairwise synthetic genetic interactions (SGI). First, a high-coverage and high-precision functional gene network (FGN) is constructed by integrating protein-protein interaction (PPI), protein complex and gene expression data; then, a graph-based semi-supervised learning (SSL) classifier is utilized to identify SGI, where the topological properties of protein pairs in weighted FGN is used as input features of the classifier. We compare the proposed SSL method with the state-of-the-art supervised classifier, the support vector machines (SVM), on a benchmark dataset in S. cerevisiae to validate our method's ability to distinguish synthetic genetic interactions from non-interaction gene pairs. Experimental results show that the proposed method can accurately predict genetic interactions in S. cerevisiae (with a sensitivity of 92% and specificity of 91%). Noticeably, the SSL method is more efficient than SVM, especially for very small training sets and large test sets.

Conclusions

We developed a graph-based SSL classifier for predicting the SGI. The classifier employs topological properties of weighted FGN as input features and simultaneously employs information induced from labelled and unlabelled data. Our analysis indicates that the topological properties of weighted FGN can be employed to accurately predict SGI. Also, the graph-based SSL method outperforms the traditional standard supervised approach, especially when used with small training sets. The proposed method can alleviate experimental burden of exhaustive test and provide a useful guide for the biologist in narrowing down the candidate gene pairs with SGI. The data and source code implementing the method are available from the website: http://home.ustc.edu.cn/~yzh33108/GeneticInterPred.htm
Predicting nucleosome positioning using a duration Hidden Markov Model
Authors: Xi Liqun , Fondufe-Mittendorf Yvonne , Xia Lei , Flatow Jared , Widom Jonathan , Wang Ji-Ping
Published: June 2010

Background

The nucleosome is the fundamental packing unit of DNAs in eukaryotic cells. Its detailed positioning on the genome is closely related to chromosome functions. Increasing evidence has shown that genomic DNA sequence itself is highly predictive of nucleosome positioning genome-wide. Therefore a fast software tool for predicting nucleosome positioning can help understanding how a genome's nucleosome organization may facilitate genome function.

Results

We present a duration Hidden Markov model for nucleosome positioning prediction by explicitly modeling the linker DNA length. The nucleosome and linker models trained from yeast data are re-scaled when making predictions for other species to adjust for differences in base composition. A software tool named NuPoP is developed in three formats for free download.

Conclusions

Simulation studies show that modeling the linker length distribution and utilizing a base composition re-scaling method both improve the prediction of nucleosome positioning regarding sensitivity and false discovery rate. NuPoP provides a user-friendly software tool for predicting the nucleosome occupancy and the most probable nucleosome positioning map for genomic sequences of any size. When compared with two existing methods, NuPoP shows improved performance in sensitivity.
"Does replication groups scoring reduce false positive rate in SNP interaction discovery?: Response"
Authors: Gayán Javier , González-Pérez Antonio , Ruiz Agustín
Published: June 2010

A response to Toplak et al: Does replication groups scoring reduce false positive rate in SNP interaction discovery? BMC Genomics 2010, 11:58.

Background

The genomewide evaluation of genetic epistasis is a computationally demanding task, and a current challenge in Genetics. HFCC (Hypothesis-Free Clinical Cloning) is one of the methods that have been suggested for genomewide epistasis analysis. In order to perform an exhaustive search of epistasis, HFCC has implemented several tools and data filters, such as the use of multiple replication groups, and direction of effect and control filters. A recent article has claimed that the use of multiple replication groups (as implemented in HFCC) does not reduce the false positive rate, and we hereby try to clarify these issues.

Results/Discussion

HFCC uses, as an analysis strategy, the possibility of replicating findings in multiple replication groups, in order to select a liberal subset of preliminary results that are above a statistical criterion and consistent in direction of effect. We show that the use of replication groups and the direction filter reduces the false positive rate of a study, although at the expense of lowering the overall power of the study. A post-hoc analysis of these selected signals in the combined sample could then be performed to select the most promising results.

Conclusion

Replication of results in independent samples is generally used in scientific studies to establish credibility in a finding. Nonetheless, the combined analysis of several datasets is known to be a preferable and more powerful strategy for the selection of top signals. HFCC is a flexible and complete analysis tool, and one of its analysis options combines these two strategies: A preliminary multiple replication group analysis to eliminate inconsistent false positive results, and a post-hoc combined-group analysis to select the top signals.
A short upstream promoter region mediates transcriptional regulation of the mouse doublecortin gene in differentiating neurons
Authors: Piens Marie , Muller Marc , Bodson Morgan , Baudouin Gregory , Plumier Jean-Christophe
Published: June 2010

Background

Doublecortin (Dcx), a MAP (Microtubule-Associated Protein), is transiently expressed in migrating and differentiating neurons and thereby characterizes neuronal precursors and neurogenesis in developing and adult neurogenesis. In addition, reduced Dcx expression during development has been related to appearance of brain pathologies. Here, we attempt to unveil the molecular mechanisms controlling Dcx gene expression by studying its transcriptional regulation during neuronal differentiation.

Results

To determine and analyze important regulatory sequences of the Dcx promoter, we studied a putative regulatory region upstream from the mouse Dcx coding region (pdcx 2kb) and several deletions thereof. These different fragments were used in vitro and in vivo to drive reporter gene expression. We demonstrated, using transient expression experiments, that pdcx 2kb is sufficient to control specific reporter gene expression in cerebellar cells and in the developing brain (E14.5). We determined the temporal profile of Dcx promoter activity during neuronal differentiation of mouse embryonic stem cells (mESC) and found that transcriptional activation of the Dcx gene varies along with neuronal differentiation of mESC. Deletion experiments and sequence comparison of Dcx promoters across rodents, human and chicken revealed the importance of a highly conserved sequence in the proximal region of the promoter required for specific and strong expression in neuronal precursors and young neuronal cells. Further analyses revealed the presence in this short sequence of several conserved, putative transcription factor binding sites: LEF/TCF (Lymphoid Enhancer Factor/T-Cell Factor) which are effectors of the canonical Wnt pathway; HNF6/OC2 (Hepatocyte Nuclear Factor-6/Oncecut-2) members of the ONECUT family and NF-Y/CAAT (Nuclear Factor-Y).

Conclusions

Studies of Dcx gene regulatory sequences using native, deleted and mutated constructs suggest that fragments located upstream of the Dcx coding sequence are sufficient to induce specific Dcx expression in vitro: in heterogeneous differentiated neurons from mESC, in primary mouse cerebellar neurons (PND3) and in organotypic slice cultures. Furthermore, a region in the 3'-end region of the Dcx promoter is highly conserved across several species and exerts positive control on Dcx transcriptional activation. Together, these results indicate that the proximal 3'-end region of the mouse Dcx regulatory sequence is essential for Dcx gene expression during differentiation of neuronal precursors.