bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploring the Expression and Prognostic Value of the TCP1 Ring Complex in Hepatocellular Carcinoma and Overexpressing Its Subunit 5 Promotes HCC Tumorigenesis
Authors: Liu Jiahui , Huang Ling , Zhu Yi , He Yongyin , Zhang Weiyun , Lei Ting , Xuan Junfeng , Xiao Bin , Li Linhai , Zhou Quan , Sun Zhaohui
Published: October 2021

T-complex protein-1 ring complex (TRiC), also known as Chaperonin Containing T-complex protein-1 (CCT), is a multisubunit chaperonin required for the folding of nascent proteins. Mounting evidence suggests that TRiC also contributes to the development and progression of tumors, but there are limited studies on pathogenic functions in hepatocellular carcinoma (HCC). We comprehensively evaluated the expression pattern and biological functions of TRiC subunits using The Cancer Genome Atlas and The Human Protein Atlas. Expression levels of TRiC subunits TCP1, CCT2/3/4/5/6A/7/8 were significantly upregulated in HCC tissues at both transcript and protein levels, which predicted shorter overall survival (OS). Moreover, high mutation rates were found in several CCT subunits, and patients with altered CCT genes exhibited poorer clinical outcomes. Functional enrichment analysis showed that co-regulated genes were preferentially involved in ‘protein folding’ and ‘microtubule-based process’, while genes co-expressed with CCT subunits were primarily involved in ‘ribosome’ and ‘spliceosome’. Knockout of CCT5 in a HCC cell line reduced while overexpression enhanced proliferation rate, cycle transition, migration, and invasion. In conclusion, these findings suggest that subunits of the TRiC may be potential biomarkers for the diagnosis of HCC and play an important role in the occurrence and development of HCC.
MiR-873-5p: A Potential Molecular Marker for Cancer Diagnosis and Prognosis
Authors: Zou Yuhao , Zhong Chenming , Hu Zekai , Duan Shiwei
Published: October 2021

miR-873 is a microRNA located on chromosome 9p21.1. miR-873-5p and miR-873-3p are the two main members of the miR-873 family. Most studies focus on miR-873-5p, and there are a few studies on miR-873-3p. The expression level of miR-873-5p was down-regulated in 14 cancers and up-regulated in 4 cancers. miR-873-5p has many targeted genes, which have unique molecular functions such as catalytic activity, transcription regulation, and binding. miR-873-5p affects cancer development through the PIK3/AKT/mTOR, Wnt/β-Catenin, NF-κβ, and MEK/ERK signaling pathways. In addition, the target genes of miR-873-5p are closely related to the proliferation, apoptosis, migration, invasion, cell cycle, cell stemness, and glycolysis of cancer cells. The target genes of miR-873-5p are also related to the efficacy of several anti-cancer drugs. Currently, in cancer, the expression of miR-873-5p is regulated by a variety of epigenetic factors. This review summarizes the role and mechanism of miR-873-5p in human tumors shows the potential value of miR-873-5p as a molecular marker for cancer diagnosis and prognosis.
One-Week Effects of Antibiotic Treatment on Gut Microbiota of Late Neonates With Pneumonia or Meningitis
Authors: Han Shujing , Zhang Qiaoru , Ding Yijun , Chu Ping , Zhang Jinjing , Shi Jin , Jia Shengnan , Yang Caiyun , Lu Jie , Wang Yajuan
Published: October 2021

Background: The neonatal period is a critical period for the establishment of the intestinal microbial community. Antibiotics can change the composition of gut microbiota.

Methods: Fecal samples were collected from 14 patients with pneumonia and 14 patients with meningitis before and after antibiotic treatment, and fecal samples from five healthy neonates at the 14th and 21st days after birth were collected as well. DNA of fecal samples was extracted, and PCR amplification was performed targeting the V3–V4 variable region of 16S rDNA. After detection by high-throughput sequencing, OTU (operational taxonomic unit) clustering, species annotation, and α diversity analysis were calculated and analyzed statistically.

Results: In the healthy control group, the abundance of Bifidobacterium increased significantly from 16.75 to 40.42%, becoming the most dominant bacteria. The results of α diversity analysis suggested that the Sobs indexes of the gut microbiota in the pneumonia and meningitis groups were significantly lower than that in the healthy control group (p < 0.05). PCoA analysis showed that the gut microbiota of pneumonia and meningitis groups clustered distinctly with the control group (Adonis p = 0.001, R2 = 0.565), and there was no significant change in the diversity of gut microbiota before and after the use of antibiotics.

Conclusions: The gut microbiota of neonates with infectious diseases were mainly related to the disease conditions. The initial state of neonatal gut microbiome determines its state after 1-week antibiotic treatment. Antibiotic application with 7 days had little effect on the community richness and some effect on the composition of gut microbiota of neonates with pneumonia or meningitis.
Clinical Attributes and Electroencephalogram Analysis of Patients With Varying Alpers’ Syndrome Genotypes
Authors: Li Hua , Wang Wei , Han Xiaodi , Zhang Yujia , Dai Lifang , Xu Manting , Deng Jie , Ding Changhong , Wang Xiaohui , Chen Chunhong , Yang Xiaofeng , Fang Fang
Published: October 2021

Alpers’ syndrome is an early inceptive neurodegenerative disorder with a poor prognosis, characterized by developmental regression, intractable epilepsy, and hepatic dysfunction. Candidate genes, such as POLG, PARS2, CARS2, FARS2, NARS2, and GABRB2 are distinguished and registered following research on large cohorts that portray the clinical phenotype in such patients using expanded access to whole-exome sequencing (WES). In this study, we aimed to better understand the electroencephalogram (EEG) characteristics and clinical phenotype of different genotypes of the Alpers’ syndrome, which are currently insufficiently studied. We conducted a study on seven patients with Alpers’ syndrome who received treatment in Beijing Children’s Hospital and had a detailed clinical EEG. Furthermore, a substantial literature search of the Chinese Biomedical Literature Database, PubMed, and Cochrane Central Register of Controlled Trials EMBASE was also conducted, which revealed a total of 22 reported cases between January 2008 to January 2021. We analyzed 29 cases of Alpers’ syndrome caused by different gene variants, of which 22 cases were related to POLG gene mutation and 7 cases were related to PARS2, CARS2, FARS2, NARS2, and GABRB2 gene mutation, and found that patients with distinctive pathogenic variants exhibited comparable phenotypes and similar EEG patterns. And we defined EEG characteristics found specifically in Alpers’ syndrome. Rhythmic high-amplitude delta with superimposed (poly) spikes (RHADS) is a characteristic EEG finding in the early stages of Alpers’ syndrome and is a kind of epileptic phenomenon, which can provide clues for the early diagnosis of the disease.
Model Based Identification of Linezolid Exposure–toxicity Thresholds in Hospitalized Patients
Authors: Fang Jie , Zhang Xiao-Shan , Zhang Chun-Hong , Zhou Zi-Ye , Han Lu , Wang Ye-Xuan , He Xiao-Shuang , Bian Xiao-Lan , Lin Guan-Yang , Jiao Zheng , Dai Ying , Yu Xu-Ben , Pan Jing-Ye
Published: October 2021

Evidence supports linezolid therapeutic drug monitoring as the exposure–response relationship has been identified for toxicity among patients receiving linezolid, but the data to establish the upper limit are limited and the published toxicity thresholds range widely. The purpose of this study was to determine the linezolid exposure–toxicity thresholds to improve the safety of linezolid. This is a multicenter retrospective study of adult patients treated with linezolid from 2018 to 2019. The population pharmacokinetic model of linezolid was established based on 270 plasma concentrations in 152 patients, which showed creatinine clearance and white cell count are covariates affecting the clearance of linezolid, and serum albumin is the covariate affecting the volume of distribution. Classification and regression tree analysis was used to determine the linezolid exposure thresholds associated with an increased probability of toxicity. Among 141 patients included for toxicity analysis, the rate of occurring toxicity was significantly higher among patients with an AUC0-24, d1 ≥163 mg h/L, AUC0-24, d2 ≥207 mg h/L, AUC0-24, ss ≥210 mg h/L, and Cmin,d2 ≥6.9 mg/L, Cmin,ss ≥6.9 mg/L, while no threshold was discovered for Cmin, d1. Those exposure thresholds and duration of linezolid treatment were independently associated with linezolid-related toxicity in the logistic regression analyses. In addition, the predictive performance of the AUC0-24 and Cmin thresholds at day 2 and steady state were close. Considering that the AUC estimation is cumbersome, Cmin threshold at 48 h and steady state with a value of ≥6.9 mg/L is recommended to improve safety, especially for patients with renal insufficiency and patients with low serum albumin.
Germacrone Attenuates Hepatic Stellate Cells Activation and Liver Fibrosis via Regulating Multiple Signaling Pathways
Authors: Li Zhiyong , Wang Zhilei , Dong Fang , Shi Wei , Dai Wenzhang , Zhao Jing , Li Qiang , Fang Zhi-e , Ren Lutong , Liu Tingting , Wei Ziying , Mou Wenqing , Lin Li , Yang Yan , Xiao Xiaohe , Ma Li , Bai Zhaofang
Published: October 2021

Liver fibrosis is an abnormal proliferation of connective tissue in the liver caused by various pathogenic factors. Chronic liver injury leads to release of inflammatory cytokines and reactive oxygen species (ROS) from damaged hepatocytes, which activates hepatic stellate cells (HSCs) to secrete extracellular matrix proteins, thereby leading to fibrosis. Thus, inhibition of hepatocyte injury and HSC activation, and promotion of apoptosis of activated HSCs are important strategies for prevention of liver fibrosis. In this study, we showed that the germacrone (GER), the main component in the volatile oil of zedoary turmeric, inhibited hepatic fibrosis by regulating multiple signaling pathways. First, GER improved the cell survival rate by inhibiting the production of ROS after hepatocyte injury caused by acetaminophen (APAP). In addition, GER inhibited the activation of HSCs and expression of collagen I by blocking TGF-β/Smad pathway in LX-2 cells. However, when the concentration of GER was higher than 60 μM, it specifically induced HSCs apoptosis by promoting the expression and activation of apoptosis-related proteins, but it had no effect on hepatocytes. Importantly, GER significantly attenuated the methionine- and choline-deficient (MCD) diet-induced liver fibrosis by inhibiting liver injury and the activation of HSCs in vivo. In summary, GER can not only protect hepatocytes by reducing ROS release to avoid the liver injury-induced HSC activation, but also directly inhibit the activation and survival of HSCs by regulating TGF-β/Smad and apoptosis pathways. These results demonstrate that GER can be used as a potential therapeutic drug for the treatment of liver fibrosis.
Analyzing Levels of Concern About Joint Punishment for Dishonesty Using the Visibility Graph Network
Authors: Qu Zhiqiang , Zhang Yujie , Li Fan
Published: October 2021

Joint punishment for dishonesty is an important means of administrative regulation. This research analyzed the dynamic characteristics of time series data from the Baidu search index using the keywords “joint punishment for dishonesty” based on a visibility graph network. Applying a visibility graph algorithm, time series data from the Baidu Index was transformed into complex networks, with parameters calculated to analyze the topological structure. Results showed differences in the use of joint punishment for dishonesty in certain provinces by calculating the parameters of the time series network from January 1, 2020 to May 27, 2021; it was also shown that most of the networks were scale-free. Finally, the results of K-means clustering showed that the 31 provinces (excluding Hong Kong, Macao and Taiwan) can be divided into four types. Meanwhile, by analyzing the national Baidu Index data from 2020 to May 2021, the period of the time series data and the influence range of the central node were found.
Comparative Study of the Effects of Tai Chi and Square Dance on Immune Function, Physical Health, and Life Satisfaction in Urban Empty-Nest Older Adults
Authors: Su ZhongJun , Zhao JieXiu
Published: October 2021

Objective:To compare the effects of Tai Chi and Square dance on immune function, physical health, and life satisfaction in urban, empty-nest older adults.

Methods:This cross-sectional study included 249 older adults (60–69 years) who were categorized into Tai Chi (n= 81), Square dance (n= 90), and control groups (n= 78). We evaluated immunoglobulin G (IgG) and interleukin-2 (IL-2) levels by enzyme-linked immunosorbent assay (ELISA), natural killer (NK) cell cytotoxicity by MTT assay, physical health indices by physical fitness levels, and life satisfaction by Life Satisfaction Index A (LSIA) scores.

Results:Immune function, physical health, and life satisfaction in older adults in the Tai Chi and Square dance groups were significantly better than those in the control group (P< 0.05). Regarding immune function and physical health, the Tai Chi group exhibited significantly higher levels of IgG (15.41 ± 0.26 g/L vs. 11.99 ± 0.35 g/L,P< 0.05), IL-2 (4.60 ± 0.20 ng/mL vs. 4.45 ± 0.21 ng/mL,P< 0.05), and NK cell cytotoxicity (0.28 ± 0.02 vs. 0.22 ± 0.02,P< 0.05) than the square dance group, significantly lower waist-to-hip ratio (0.87 ± 0.02 vs. 0.89 ± 0.02,P< 0.05), resting pulse (78.4 ± 4.6 beats/min vs. 81.0 ± 3.1 beats/min,P< 0.05), systolic blood pressure (132.0 ± 5.2 mmHg vs. 136.2 ± 3.2 mmHg,P< 0.05), diastolic blood pressure (80.0 ± 2.6 mmHg vs. 83.0 ± 2.7 mmHg,P< 0.05), and significantly higher vital capacity (2978.0 ± 263.0 mL vs. 2628.3 ± 262.8 mL,P< 0.05) and duration of one-leg standing with eyes closed (16.2 ± 1.9 s vs. 12.0 ± 1.7 s). However, there was no significant difference in LSIA scores between the Tai Chi and Square dance groups (12.05 ± 1.96 vs. 13.07 ± 1.51,P> 0.05). Further, there was a significant correlation between LSIA scores and immune function (r= 0.50,P= 0.00) and physical health (r= 0.64,P= 0.00).

Conclusion:(1) Both Tai Chi and square dance practitioners had better health outcomes, compared with sedentary individuals; (2) Tai Chi practitioners had better physical health and immune function than Square dance practitioners. (3) Tai Chi and Square dance exercises had similar effects on life satisfaction among urban empty-nest older adults.

Suggestions:For urban empty-nest older adults who want to have better physical health and immune function, long-term Tai Chi exercise may be a better choice; however, those who are concerned about life satisfaction can choose either Tai Chi or Square dance exercise.
Shaping the Organ: A Biologist Guide to Quantitative Models of Plant Morphogenesis
Authors: Marconi Marco , Wabnik Krzysztof
Published: October 2021

Organ morphogenesis is the process of shape acquisition initiated with a small reservoir of undifferentiated cells. In plants, morphogenesis is a complex endeavor that comprises a large number of interacting elements, including mechanical stimuli, biochemical signaling, and genetic prerequisites. Because of the large body of data being produced by modern laboratories, solving this complexity requires the application of computational techniques and analyses. In the last two decades, computational models combined with wet-lab experiments have advanced our understanding of plant organ morphogenesis. Here, we provide a comprehensive review of the most important achievements in the field of computational plant morphodynamics. We present a brief history from the earliest attempts to describe plant forms using algorithmic pattern generation to the evolution of quantitative cell-based models fueled by increasing computational power. We then provide an overview of the most common types of “digital plant” paradigms, and demonstrate how models benefit from diverse techniques used to describe cell growth mechanics. Finally, we highlight the development of computational frameworks designed to resolve organ shape complexity through integration of mechanical, biochemical, and genetic cues into a quantitative standardized and user-friendly environment.
Influential Factors on Collective Anxiety of Online Topic-Based Communities
Authors: Yang Yi , Ta Na , Li Kaiyu , Jiao Fang , Hu Baijing , Li Zhanghao
Published: October 2021

Background: Under the uncertainty led by the decentralized information on social media, people seek homogeneity in either opinions or affection to establish group identity to better understand the information. This also means they are easily polarized, not only ideologically but also in their actions. Affective polarization is the emotional tendency for people to show animosity toward opposing partisans while seeking homogeneity from fellow partisans. Much research into online affective polarization has focused on quantifying anxiety at an individual level while neglecting that on a collective basis. Therefore, this paper examined the polarization of collective anxiety in topic-based communities on Weibo.

Methods: We aim to interpret correlations between collective anxiety online and topic characteristics, user competence, as well as the proportion of influencers of Weibo topic-based communities. Our neural networks model and statistical analysis were based on 200 communities with 403,380 personal accounts and 1,012,830 messages.

Results: Collective anxiety levels are correlated to (1) the extent to which a topic captures public interest, (2) how community members articulate topics on social network platforms, and (3) the ratio of influencers in the community. Specifically, people’s conflicting perceptions and articulations of topics might increase collective anxiety, while the extent to which a topic is of the public interest and the number of influencers engaged in a topic account for any decline in its ranking. Furthermore, familiarity with a topic does not help predict collective anxiety levels. There are no significant links between community size or interactivity dynamics and the level of collective anxiety in the topic-based community. Our computational model has 85.00% precision and 87.00% recall.

Conclusion: This study found the collective anxiety augment due to topic proximities to public interest and members’ lack of declarative knowledge on topics, while to decline with an increasing portion of online influencers. These findings indicate that collective anxiety is induced due to a lack of credibility. Also, the amount of conflicting information shared by different people places them in a state of flux. Therefore, a community with more influencers may be more likely to experience anxiety polarization, bringing forth the issue of layered information and inequality.
Effectiveness Between Daily and After-Each-Case Room Disinfection of the Endoscopy Unit
Authors: Jin Bo , Hu Yue , Huang Liang , Cheng Xiaoyun , Zhao Jin , Yang Xuejing , Sun Xiling , Gan Tieer , Lu Bin
Published: October 2021

Background: To evaluate the effectiveness between daily and after-each-case room disinfection in the endoscopy unit.

Methods: This study was conducted in an endoscopy unit of the First Affiliation of Zhejiang Chinese Medical University. We cultured samples from the surface of endoscopy unit items, including operation unit air, isolation gown of an endoscopist, control panel buttons, workstation mouse, and the bed head of the patient. All the samples were divided into daily and after-each-case room disinfection groups. In addition, each group was subdivided into sedation and nonsedation gastroscopy with and without ventilation room groups.

Results: The qualified rate of bed head samples of the patient were lower in the daily room disinfection group (76.67%) compared with the after-each-case group (100%). The isolation gown, mouse at the workstation, and the bed head of the patient demonstrated the lowest bacterial and fungal load in the after-each-case room disinfection group compared with the daily room disinfection group (p < 0.05). In the subgroup analysis, a higher microbial load was observed for the isolation gown of the endoscopist used during nonsedation gastroscopy in an unventilated room under the after-each-case room disinfection pattern (p < 0.05); a higher microbial load was observed for the control panel buttons used during nonsedation gastroscopy under the after-each-case room disinfection pattern (p < 0.05).

Conclusions: For risk-free or low-risk patients, daily room disinfection provides the basic health requirements of the endoscopy procedure. However, it is better to adopt the after-each-case room disinfection for the isolation gown of the endoscopist and bed head of the patient. For the patients with high risk, the after-each-case room disinfection is more suitable for every endoscopy unit (www.ClinicalTrials.gov, NCT04399005).
The Expected Demand for Elderly Care Services and Anticipated Living Arrangements Among the Oldest Old in China Based on the Andersen Model
Authors: Zeng Yanbing , Que Shuang , Lin Chenxi , Fang Ya
Published: October 2021

Objective: The first aim of this study was to explore expected demands of the oldest old and their determinants for different types of elderly care services. The second aim was to investigate preferred choices of living arrangements among the oldest old and the influencing factors.

Methods: Data of 4,738 participants aged ≥80 years were extracted from the Chinese Longitudinal Health Longevity Survey carried out in 2014. Using the Andersen model as the analysis framework, a multiple logistic regression analysis was performed to analyze the relationship between the expected elderly care services and living arrangements and other influencing factors. The odds ratios were calculated to indicate the relationship between the influencing factors and the dependent variables.

Results: From the descriptive analysis results, we found that the oldest old showed high anticipated needs for home visits (83.5%) and health education (76.4%). Further, there existed a huge imbalance between the supply and demand of care services for the aged. Living with children is still the most important way of providing for the oldest old. The regression results showed that the expected demands for elderly care services and anticipated living arrangements among the oldest old in China are influenced by age, residence, housing property rights, economic status, loneliness, and activities of daily living (ADLs). The oldest old who are older without housing property rights, childless, and have restricted ADLs were more frequently observed to live in long-term care institutions.

Conclusions: There is an inequality of the supply and expected demand for elderly care services, and living with children is still a preferred choice of the Chinese oldest old. Our findings indicate that when planning how to promote elderly care services among the oldest old, it is important to consider their expectations, especially for the subgroup that is relatively disadvantaged. Related policies should be developed to offer incentives to family caregivers when they live with the oldest old.
The SMOOTH-Robot: A Modular, Interactive Service Robot
Authors: Krüger Norbert , Fischer Kerstin , Manoonpong Poramate , Palinko Oskar , Bodenhagen Leon , Baumann Timo , Kjærum Jens , Rano Ignacio , Naik Lakshadeep , Juel William Kristian , Haarslev Frederik , Ignasov Jevgeni , Marchetti Emanuela , Langedijk Rosalyn Melissa , Kollakidou Avgi , Jeppesen Kasper Camillus , Heidtmann Conny , Dalgaard Lars
Published: October 2021

The SMOOTH-robot is a mobile robot that—due to its modularity—combines a relatively low price with the possibility to be used for a large variety of tasks in a wide range of domains. In this article, we demonstrate the potential of the SMOOTH-robot through three use cases, two of which were performed in elderly care homes. The robot is designed so that it can either make itself ready or be quickly changed by staff to perform different tasks. We carefully considered important design parameters such as the appearance, intended and unintended interactions with users, and the technical complexity, in order to achieve high acceptability and a sufficient degree of utilization of the robot. Three demonstrated use cases indicate that such a robot could contribute to an improved work environment, having the potential to free resources of care staff which could be allocated to actual care-giving tasks. Moreover, the SMOOTH-robot can be used in many other domains, as we will also exemplify in this article.
Environmental Heat Exposure Among Pet Dogs in Rural and Urban Settings in the Southern United States
Authors: Moon Katherine E. , Wang Suwei , Bryant Kaya , Gohlke Julia M.
Published: October 2021

With advancing global climate change, heat-related illnesses and injuries are anticipated to become more prevalent for humans and other species. Canine hyperthermia is already considered an important seasonal emergency. Studies have been performed on the risk factors for heat stroke in canine athletes and military working dogs; however there is limited knowledge on environmental risk factors for the average pet dog. This observational study explores variation in individually experienced environmental temperatures of pet dogs (N = 30) in rural and urban environments in central Alabama. Temperature data from dogs and their owners was collected using wearable personal thermometers. Demographic data on the dogs was collected using a brief survey instrument completed by their owners. Dogs included in the study varied in signalment, activity level, and home environment. Linear mixed effects regression models were used to analyze repeated measure temperature and heat index values from canine thermometers to explore the effect of environmental factors on the overall heat exposure risk of canine pets. Specifically, the heat exposures of dogs were modeled considering their owner's experienced temperatures, as well as neighborhood and local weather station measurements, to identify factors that contribute to the heat exposure of individual dogs, and therefore potentially contribute to heat stress in the average pet dog. Results show hourly averaged temperatures for dogs followed a diurnal pattern consistent with both owner and ambient temperature measurements, except for indoor dogs whose recordings remained stable throughout the day. Heat index calculations showed that owners, in general, had more hours categorized into the National Weather Station safe category compared to their dogs, and that indoor dogs had a greater proportion of hours categorized as safe compared to outdoor dogs. Our results suggest that the risk of the average pet dog to high environmental heat exposure may be greater than traditional measures indicate, emphasizing that more localized considerations of temperature are important when assessing a dog's environmental risk for heat-related injury or illness.
Stabilization of Switched Time-Delay Linear Systems through a State-Dependent Switching Strategy
Authors: Hou Tan , Li Yuanlong , Lin Zongli
Published: October 2021

This paper considers the problem of stabilizing switched time-delay linear systems through a state-dependent switching strategy. In contrast to the existing works, we adopt a less restrictive assumption of the system, and show that this assumption is sufficient to guarantee asymptotic stability of the considered system under the min-projection switching strategy. Our results also imply that the min-projection switching strategy, originally designed for delay-free switched systems, is robust with respect to small state delays. An optimization problem is formulated to estimate the upper bound of the tolerable time delay. Numerical examples are presented to show that our method is applicable to a larger class of switched systems and leads to a greater delay bound.
Customer Preferences for Organic Agriculture Produce in the Czech Republic: 2016 and 2019
Authors: Zámková Martina , Rojík Stanislav , Pilař Ladislav , Chalupová Martina , Prokop Martin , Stolín Radek , Dziekański Paweł , Maitah Mansoor
Published: October 2021

The article analyses the customer attitude towards the qualities and benefits of organic agriculture production for farmers and customers in the Czech Republic, comparing the situation in 2016 and 2019. More than 2500 respondents were subject to the marketing research in the years 2016 and 2019. The data were processed using correspondence analysis and logistic regression. The research study shows that the number of respondents who consider organic food is growing; at the same time, there is a rather large share of consumers who believe organic food to be of better quality. The results show a favourable change in the popularity of organic food. While, in 2016, the main decisive factor in shopping for organic food was its price, in 2019, the main criterion, for the respondents, was quality, with the criterion of price being complemented by the perception of organic food as healthier than conventional food. At the same time, it was established that, the amount spent on organic food in 2019 was higher than that in 2016. This finding was in positive correlation with the increase in respondents’ income. For farmers, organic farming is a promising alternative to conventional agriculture due to a rising demand for organic produce.
Conceptualizing Indigenous Human–Animal Relationships in Aotearoa New Zealand: An Ethical Perspective
Authors: Woodhouse Jordan , Carr Anna , Liebergreen Nicola , Anderson Lynley , Beausoleil Ngaio J. , Zobel Gosia , King Mike
Published: October 2021

This article considers the complexity and diversity of ethical concepts and beliefs held by Māori, the indigenous people of Aotearoa New Zealand (hereafter New Zealand), relating to animals. A combination of interviews and focus group discussions were conducted with individuals who identify as Māori and were working with wildlife, primarily in an eco-tourism and conservation context. Two main themes emerged from the data: ethical concepts relating to the environment, and concepts relating to the spiritual relationships between people, animals and the environment. These findings highlight that the connections between humans and animals through a Māori lens are nuanced in ways not typically accounted for in Western philosophy. This is of particular importance because of the extent to which standard Western thought is embodied in law and policy related to human treatment of animals and the environment. In New Zealand, relationships and partnerships are informed by Te Tiriti ō Waitangi, one of New Zealand’s founding documents. Where these partnerships include activities and environments involving human–animal interaction, policy and legislation should account for Māori knowledge, and diverse of thought among different hapū (tribal groups). We conclude by exploring ways of including Māori ethical concepts around animals in general, and wild animals in particular, in law and policy, providing a case study relevant to other bicultural or multicultural societies.
Early Detection of Locomotion Disorders in Gilts Using a Novel Visual Analogue Scale; Associations with Chronic Stress and Reproduction
Authors: Lagoda Martyna E. , Boyle Laura A. , Marchewka Joanna , O’Driscoll Keelin
Published: October 2021

Locomotion scoring is crucial for the early detection of lameness, which reduces sow welfare and performance. Our objective was to test the effectiveness of a visual analogue scale (VAS) to measure locomotory ability (OVERALL) compared to a categorical scoring system (CAT) and to investigate associations with hair cortisol and reproductive performance. Locomotion was scored in gilts (n = 51) at service, on day 57 and day 108 of pregnancy, and at weaning, using a VAS (150 mm line: 0 mm (perfect)–150 mm (severely lame)), and a CAT (1 (perfect)–5 (severely lame)). Hair cortisol concentration was measured on day 108 of pregnancy. Reproductive performance data (parity 1–4) were acquired from farm records. VAS detected deviations from optimal locomotion more effectively than the CAT (F3,145 = 2.70; p ≤ 0.05 versus F3,195 = 2.45; p = 0.065). Higher OVERALL scores at service (REG = 0.003 ± 0.0012; F1,48 = 4.25; p ≤ 0.05) and on day 57 (REG = 0.003 ± 0.0013; F1,48 = 6.95; p ≤ 0.05) were associated with higher hair cortisol concentrations on day 108. Positive associations were detected between OVERALL at service and the number of piglets born dead (REG = 0.01 ± 0.006; F1,36 = 4.24; p ≤ 0.05), and total born (REG = 0.1 ± 0.03; F1,120 = 4.88; p ≤ 0.05). The VAS better facilitates early detection of lameness, which could help to prevent detrimental effects, possibly mediated by chronic stress, on reproductive performance.
Antibacterial, Antifungal and Anticancer Activities of Compounds Produced by Newly Isolated Streptomyces Strains from the Szczelina Chochołowska Cave (Tatra Mountains, Poland)
Authors: Jaroszewicz Weronika , Bielańska Patrycja , Lubomska Daria , Kosznik-Kwaśnicka Katarzyna , Golec Piotr , Grabowski Łukasz , Wieczerzak Ewa , Dróżdż Weronika , Gaffke Lidia , Pierzynowska Karolina , Węgrzyn Grzegorz , Węgrzyn Alicja
Published: October 2021

Resistance of bacteria, fungi and cancer cells to antibiotics and other drugs is recognized as one of the major problems in current medicine. Therefore, a search for new biologically active compounds able to either kill pathogenic cells or inhibit their growth is mandatory. Hard-to-reach habitats appear to be unexplored sources of microorganisms producing previously unknown antibiotics and other molecules revealing potentially therapeutic properties. Caves belong to such habitats, and Actinobacteria are a predominant group of microorganisms occurring there. This group of bacteria are known for production of many antibiotics and other bioactive compounds. Interestingly, it was demonstrated previously that infection with bacteriophages might enhance production of antibiotics by them. Here, we describe a series of newly isolated strains of Actinobacteria that were found in caves from the Tatra Mountains (Poland). Phage induction tests indicated that some of them may bear active prophages able to produce virions upon treatment with mitomycin C or UV irradiation. Among all the examined bacteria, two newly isolated Streptomyces sp. strains were further characterized to demonstrate their ability to inhibit the growth of pathogenic bacteria (strains of Staphylococcus aureus, Salmonella enterica, Enterococcus sp., Escherichia coli, and Pseudomonas aeruginosa) and fungi (different species and strains from the genus Candida). Moreover, extracts from these Streptomyces strains reduced viability of the breast-cancer cell line T47D. Chemical analyses of these extracts indicated the presence of isomers of dichloranthrabenzoxocinone and 4,10- or 10,12-dichloro-3-O-methylanthrabenzoxocinone, which are putative antimicrobial compounds. Moreover, various previously unknown (unclassified) molecules were also detected using liquid chromatography–mass spectrometry, suggesting that tested Streptomyces strains may synthesize a battery of bioactive compounds with antibacterial, antifungal, and anticancer activities. These results indicate that further studies on the newly isolated Actinobacteria might be a promising approach to develop novel antibacterial, antifungal, and/or anticancer drugs.
Challenges in Steroid and Anticoagulant Therapy in Severe COVID-19 Pneumonia: A Prospective Study
Authors: Hassan Alaa Thabet , Elmoniem Alaa E. Abd , Abdelrady Marwa Mahmoud , Mohamed Mona Embarek , Mokhtar Mohamed A. , Elsherif Abdelhalim A. , Saied Ghada Mohamed , Kasem Soheir M.
Published: October 2021

Background: As COVID-19 has neither a standard treatment protocol nor guidelines, there are many treatment protocols for anti-inflammatory corticosteroids and anti-coagulations for severe COVID-19 pneumonia patients. This study aimed to assess the most suitable modality in this high-risk group. Methods: A prospective, experimental study design was adopted that included 123 severe COVID-19 pneumonia patients admitted at Assiut University Hospital. Patients were divided into three groups according to a combined corticosteroid and anticoagulants therapy protocol. Group A included 32 patients, group B included 45 patients, and group C included 46 patients. Assessment of cases was conducted according to the treatment type and duration, weaning duration from oxygen therapy, length of hospital and ICU stay, and complications during treatment. Three months follow-up after discharge was performed. Results: the three patient groups showed significant differences regarding the 3-month outcome, whereas Group C showed the highest cure rate, lowest lung fibrosis, and lowest mortality rate over the other two groups. The in-hospital outcome, the development of pulmonary embolism, bleeding, hematoma, acute kidney disease, and myocardial infarction showed a significant difference between groups (p values < 0.05). Mortality predictors among severe COVID-19 patients by multivariable Cox hazard regression included treatment modality, history of comorbid diseases, increased C reactive protein, high neutrophil-lymphocyte ratio, and shorter ICU and hospital stay. Conclusion: the use of combined methylprednisolone and therapeutic Enoxaparin, according to a flexible protocol for COVID-19 patients with severe pneumonia, had two benefits; the prevention of disease complications and improved clinical outcome.
Glyoxalase-I Is Upregulated in Acute Cerulein-Induced Pancreatitis: A New Mechanism in Pancreatic Inflammation?
Authors: Hollenbach Marcus , Sonnenberg Sebastian , Sommerer Ines , Lorenz Jana , Hoffmeister Albrecht
Published: October 2021

Inflammation caused by oxidative stress (ROS) demonstrates an essential mechanism in the pathogenesis of acute pancreatitis (AP). Important sources for ROS comprise the reactive compound methylglyoxal (MGO) itself and the MGO-derived formation of advanced glycation end-products (AGEs). AGEs bind to the transmembrane receptor RAGE and activate NF-κB, and lead to the production of pro-inflammatory cytokines. MGO is detoxified by glyoxalase-I (Glo-I). The importance of Glo-I was shown in different models of inflammation and carcinogenesis. Nevertheless, the role of Glo-I and MGO in AP has not been evaluated so far. This study analyzed Glo-I in cerulein-(CN)-induced AP and determined the effects of Glo-I knockdown, overexpression and pharmacological modulation. Methods: AP was induced in C57BL6/J mice by i.p. injection of CN. Glo-I was analyzed in explanted pancreata by Western Blot, qRT-PCR and immunohistochemistry. AR42J cells were differentiated by dexamethasone and stimulated with 100 nM of CN. Cells were simultaneously treated with ethyl pyruvate (EP) or S-p-bromobenzylglutathione-cyclopentyl-diester (BrBz), two Glo-I modulators. Knockdown and overexpression of Glo-I was achieved by transient transfection with Glo-I siRNA and pEGFP-N1-Glo-I-Vector. Amylase secretion, TNF-α production (ELISA) and expression of Glo-I, RAGE and NF-κB were measured. Results: Glo-I was significantly upregulated on protein and mRNA levels in CN-treated mice and AR42J cells. Dexamethasone-induced differentiation of AR42J cells increased the expression of Glo-I and RAGE. Treatment of AR42J cells with CN and EP or BrBz resulted in a significant reduction of CN-induced amylase secretion, NF-κB, RAGE and TNF-α. Overexpression of Glo-I led to a significant reduction of CN-induced amylase levels, NF-κB expression and TNF-α, whereas Glo-I knockdown revealed only slight alterations. Measurements of specific Glo-I activity and MGO levels indicated a complex regulation in the model of CN-induced AP. Conclusion: Glo-I is overexpressed in a model of CN-induced AP. Pharmacological modulation and overexpression of Glo-I reduced amylase secretion and the release of pro-inflammatory cytokines in AP in vitro. Targeting Glo-I in AP seems to be an interesting approach for future in vivo studies of AP.
Pseurotin D Induces Apoptosis through Targeting Redox Sensitive Pathways in Human Lymphoid Leukemia Cells
Authors: Mosejová Eva , Bosnjakovic Rebeka , Kubala Lukáš , Vašíček Ondřej
Published: October 2021

Chronic lymphocytic leukemia (CLL) is the most prevalent lymphoid malignancy in many geographical regions of the world. Pseurotin D, a secondary metabolite of fungi, represents a group of bioactive natural products with a newly ascribed range of interesting biological activities. The purpose of this study was to bring new insights into the mechanism behind the effects of pseurotin D on MEC-1 cells as a representative CLL cell line, with a particular focus on selected signaling pathways important in the proliferation of cells and targeting mitochondrial metabolism. Our results showed that pseurotin D was able to significantly inhibit the proliferation of MEC-1 cells and arrested them in the G2/M cell cycle phase. In addition, pseurotin D was able to induce apoptosis. We found that all of these effects were associated with a change in mitochondrial membrane potential and the production of mitochondrial reactive oxygen species (ROS). We showed for the first time that pseurotin D suppresses MEC-1 cell proliferation and induces apoptotic cell death via induction of the collapse of the mitochondria respiratory chain and the ROS-related caspase pathway. Our results show the pseurotins family as promising compounds which could serve as a basis for the development of new compounds in the treatment of lymphoma.
Evaluating the Rheological, Chemical and Morphological Properties of SBS Modified Asphalt-Binder under Multiple Aging and Rejuvenation Cycles
Authors: Chen Xiaobing , Ning Yunfeng , Gu Yongming , Zhao Ronglong , Tong Jinhu , Wang Juntian , Zhang Xiaorui , Wen Wei
Published: October 2021

To investigate the influence of multiple cycles of aging and rejuvenation on the rheological, chemical, and morphological properties of styrene–butadiene–styrene (SBS)-modified asphalt-binders, the asphalt-binders were aged using two laboratory simulation methods, namely a rolling thin film oven (RTFO) test for short-term aging and pressure aging vessel (PAV) for long-term aging. The asphalt-binders were then rejuvenated with three types of rejuvenators (Type I, II, and III) with different dosages (i.e., 6%, 10%, and 14% for the first, second, and third rejuvenation, respectively). A dynamic shear rheometer (DSR) was then used to analyze the effect of rejuvenators on the rheological properties of all the asphalt-binders. The changes in the functional groups and microscopic morphology in the process of multiple aging and rejuvenation cycles were studied using Fourier transform infrared (FTIR) and atomic force microscopy (AFM). The results indicated that the three rejuvenators could soften the stiffness and restore the microstructures of the aged asphalt-binders in the process of repeated aging and rejuvenation from DSR and AFM testing. Considering the rutting and fatigue properties, the Type I rejuvenator exhibited the potential to achieve the desired rejuvenation effects under multiple rejuvenation cycles. During the multiple aging and rejuvenation cycles, the aging resistance of SBSMA decreased gradually from the FTIR results. This inherently limited the number of repeated rejuvenation cycles. This research is conducive to promoting the application of repeated penetrating rejuvenation.
Explaining Bad Forecasts in Global Time Series Models
Authors: Rožanec Jože , Trajkova Elena , Kenda Klemen , Fortuna Blaž , Mladenić Dunja
Published: October 2021

While increasing empirical evidence suggests that global time series forecasting models can achieve better forecasting performance than local ones, there is a research void regarding when and why the global models fail to provide a good forecast. This paper uses anomaly detection algorithms and explainable artificial intelligence (XAI) to answer when and why a forecast should not be trusted. To address this issue, a dashboard was built to inform the user regarding (i) the relevance of the features for that particular forecast, (ii) which training samples most likely influenced the forecast outcome, (iii) why the forecast is considered an outlier, and (iv) provide a range of counterfactual examples to understand how value changes in the feature vector can lead to a different outcome. Moreover, a modular architecture and a methodology were developed to iteratively remove noisy data instances from the train set, to enhance the overall global time series forecasting model performance. Finally, to test the effectiveness of the proposed approach, it was validated on two publicly available real-world datasets.
Study to Increase the TRL of Exoskeleton ERMIS Based on a Methodology to the Identification of Real Performance Parameters
Authors: Medina-Valdes José Luis , Zúñiga-Avilés Luis Adrián , Cruz-Martínez Giorgio Mackenzie , Vilchis-González Adriana Herlinda , Silva-Rivera Usiel Sandino
Published: October 2021

Many exoskeletons in scientific communications and patents only reach a technology readiness level corresponding to an experimental physical model (EPM) or a low-fidelity prototype. While only operational in a laboratory environment, the increasing technology readiness level (TRL) in exoskeletons is not widely studied. This work presents a study to reach this aim based on a new methodology that includes two phases, eleven steps, and four case studies from EPM (TRL3) of ERMIS up to TRL 5 of ERMIS. The results of this article show the increase in TRL based on the analysis of the operational parameters of the ERMIS exoskeleton. The validation of the passive rehabilitation movements was made by characterizing the points of their trajectories assisted by an anthropomorphic mechanism used to measure the end-effector position of ERMIS by means of the acquisition of data, obtaining an error of 20 mm. In conclusion, the real performance parameters are detailed, explaining their causes according to the behavior of the exoskeleton in a real environment operating the four case studies. It presents the group of parameters that reach the TRL 5, which were validated in Computer-Aided Design (CAD) software.
Influence of Examiners’ Experience and Region of Interest Location on Semiquantitative Elastography Validity and Reliability
Authors: Valera-Calero Juan Antonio , Fernández-de-las-Peñas César , Fernández-Rodríguez Tomás , Arias-Buría José Luis , Varol Umut , Gallego-Sendarrubias Gracia María
Published: October 2021

Semi-quantitative elastography is a promising imaging technique to evaluate tissue stiffness differences, providing data regarding relative stiffness differences between two targets. The aims of this study were to assess the validity, inter-examiner reliability and variability of semi-quantitative elastography for calculating strain ratios (SR) in a homogeneous gel phantom in different locations within the image. A diagnostic accuracy study was performed in a homogeneous stiffness phantom. Four examiners participated (two novice and two experienced). Each examiner assessed the SR in two locations. Difference between examiners, variability of measurements, SR error and absolute error, mean error of the measurements and coefficient of variation were calculated. The agreement between examiners, validity and variability of measurements were higher in the central area than the lateral areas of the images. Thus, the experience of the examiner was relevant for the concordance of the measurements in the lateral areas of the images (SR difference of 0.14 ± 0.05; p < 0.001), but not for the central area (SR difference of 0.05 ± 0.02; p > 0.05). Our data suggested that semi-quantitative elastography is an accurate tool for assessing small magnitude stiffness differences within the same image in central areas, but the experience of the examiner is a determinant factor.