bluuebunny/crossref_metadata_2025 huggingface dataset:
The Green Structure for Outdoor Places in Dry, Hot Regions and Seasons—Providing Human Thermal Comfort in Sustainable Cities
Authors: Bandurski Karol , Bandurska Hanna , Kazimierczak-Grygiel Ewa , Koczyk Halina
Published: June 2020

Hot and dry climate and air pollution is a growing problem in urban areas, and this can have an adverse impact on life and health of urban residents. One of the ways to protect people from this hazard is the use of urban green or street greenery. However, its implementation can be problematic in highly urbanized areas. This paper presents a concept of the green structure (GS), designed, and is still being developed, by Adam Kalinowski where cooling efficiency is based on the synergy of shade and evapotranspiration. The GS that could be used as street furniture, small architecture form or a public utility structure intended to protect people and objects from an adverse urban environment, at the same time providing pleasant and healthy microclimate inside. The pilot project-the first application of the GS in the urban environment-is presented and the results of short-term measurements of temperature and humidity are provided and analyzed. Moreover, a simple dynamic simulation of the GS performance in courtyards has been conducted. The obtained results show the decrease of the perceived temperature within this structure. Depending on climate type, an average potential reduction of Universal Thermal Comfort Index (UTCI) and mean radiant temperature (Tmrt), caused by the GS in a courtyard case study, is 5–8 °C and 17–29 °C, respectively. Performed simulation also confirms that TRNSYS software is an appropriate tool for simple outdoor microclimate analysis. Further research to develop this concept, increase its performance and customize it for different applications are proposed.
A Comprehensive Review on IoT Protocols’ Features in Smart Grid Communication
Authors: Tightiz Lilia , Yang Hyosik
Published: June 2020

Since the smart grid deals with a large mass of data and critical missions, it requires ubiquitous, reliable, and real-time communication. The Internet of Things (IoT) technology, which has the potential of connecting all objects over the globe through the Internet, excels in providing robust information transmission infrastructure in the smart grid. There are a multitude of possible protocols, standards, and configurations for communication in the smart grid. A commonly applied communication standard IEC 61850 recommends the use of Manufacturing Message Specification (MMS) protocol for communication in Local Area Network (LAN) and eXtensible Messaging and Presence Protocol (XMPP) in Wide Area Network (WAN). However, a plethora of research on this topic compares the behavior of other IoT protocols and standard recommendations in the smart grid. On the other hand, the sky-rocketing penetration of Renewable Energy Sources (RES), especially in the form of micro grid, transformed the central control structure of the smart grid into a distributed style called Multi-Agent Systems (MAS). This new approach defined new communication requirements and more particular IoT protocol characteristic requirements. However, a limited number of the existing studies have considered IoT protocol characteristic requirements of the smart grid and its new control structures. In this paper, we initially investigate the communication requirements of the smart grid and introduce all IoT protocols and their specifications. We analyze IoT protocol characteristics and performances in the smart grid through literature review based on the smart grid communication requirements. In this approach, we highlight weak points of these practices making them fail to acquire the holistic guidelines in utilizing proper IoT protocol that can meet the smart grid environment interaction requirements. Using the existing facilities, the public Internet, we follow the arrangement of cost-effective high penetration communication requirements for new structures of the smart grid, i.e., the MAS and multi-micro grid. In this case, we consider IoT protocol Quality of Services (QoS) requirements, especially in the case of security and reliability, to satisfy stakeholders, namely utilities and prosumers. Addressing effective elements in applying IoT in the smart grid’s future trends is another contribution to this paper.
Developing Smart Energy Communities around Fishery Ports: Toward Zero-Carbon Fishery Ports
Authors: Alzahrani Ateyah , Petri Ioan , Rezgui Yacine , Ghoroghi Ali
Published: June 2020

Air quality and energy consumption are among the top ten environmental priorities in seaports as stated by the European Sea Ports Organization. Globally, it is estimated that 15% of energy consumption can be attributed to refrigeration and air conditioning systems in fishing activities. There is a real need to understand energy usage in fishery ports to help identify areas of improvements, with a view to optimize energy usage and minimize carbon emissions. In this study, we elaborate on ways in which a simulation capability can be developed at the community level with a fishery port, using a real-world case study seaport in Milford Heaven (Wales, UK). This simulation-based strategy is used to investigate the potential of renewable energy, including local solar farms, to meet the local power demand. This has informed the development of a simulation-based optimization strategy meant to explore how smart energy communities can be formed at the port level by integrating the smart grid with the local community energy storage. The main contribution of the paper involves a co-simulation environment that leverages calibrated energy simulation models to deliver an optimization capability that (a) manages electrical storage within a district an environment, and (b) promotes the formation of energy communities in a fishery port ecosystem. This is paving the way to policy implications, not only in terms of carbon and energy reduction, but also in the formation and sustained management of energy communities.
A Fast-Algorithmic Probabilistic Evaluation on Regional Rate of Change of Frequency (RoCoF) for Operational Planning of High Renewable Penetrated Power Systems
Authors: Wen Jiaxin , Bu Siqi , Zhou Bowen , Chen Qiyu , Yang Dongsheng
Published: June 2020

The high rate of change of frequency (RoCoF) issue incurred by the integration of renewable energy sources (RESs) into a modern power system significantly threatens the grid security, and thus needs to be carefully examined in the operational planning. However, severe fluctuation of regional frequency responses concerned by system operators could be concealed by the conventional assessment based on aggregated system frequency response. Moreover, the occurrence probability of a high RoCoF issue is actually a very vital factor during the system planner’s decision-making. Therefore, a fast-algorithmic evaluation method is proposed to determine the probabilistic distribution of regional RoCoF for the operational planning of a RES penetrated power system. First, an analytical sensitivity (AS) that quantifies the relationship between the regional RoCoF and the stochastic output of the RES is derived based on the generator and network information. Then a linear sensitivity-based analytical method (LSM) is established to calculate the regional RoCoF and the corresponding probabilistic distribution, which takes much less computational time when comparing with the scenario-based simulation (SBS) and involves much less complicated calculation procedure when comparing with the cumulant-based method (CBM). The effectiveness and efficiency of the proposed method are verified in a modified 16-machine 5-area IEEE benchmark system by numerical SBS and analytical CBM.
Economic Study of Solar Chimney Power-Water Distillation Plant (SCPWDP)
Authors: Abdelsalam Emad , Kafiah Feras , Alkasrawi Malek , Al-Hinti Ismael , Azzam Ahmad
Published: June 2020

This work provides technical and economic feasibility of installing a solar chimney power-water distillation plant (SCPWDP) in the port of Aqaba in Jordan. A mathematical model for the SCPWDP has been developed and validated. A full year of weather conditions data has been processed, where water and electricity productions have been estimated. The results show that the SCPWDP can produce 481,440 kWh of electricity and 123,753 ton of distilled water yearly simultaneously. The economic analysis shows that the levelized cost of energy (LCOE) for the SCPWDP is $1.86/kWh. The cost estimate seems reasonable once compared with other studies. However, from economical point of view the LCOE of SCPWDP requires a further decrease in order to make it economically feasible. The sensitivity analysis shows that increasing the chimney height of the SCPWDP would increase the production of electricity, and hence, decreases the LCOE. However, increasing the chimney height would have a marginal improvement since it increases both capital and operation expenditures.
Inkjet 3D Printed MEMS Vibrational Electromagnetic Energy Harvester
Authors: Kawa Bartosz , Śliwa Krzysztof , Lee Vincent , Shi Qiongfeng , Walczak Rafał
Published: June 2020

Three-dimensional (3D) printing is a powerful tool that enables the printing of almost unlimited geometry in a few hours, from a virtual design to a real structure. In this paper, we present a micro-electromechanical energy harvester that utilized a 3D printed micromechanical structure combined with a miniature permanent magnet and a microelectronic coil towards a hybrid electromagnetic vibrational hybrid energy harvester. Various micromechanical structure geometries were designed, printed, and tested. The characteristic dimensions of the springs were from 200 μm to 400 μm and the total volume of the devices was below 1 cm3. The resonant frequencies (95–340 Hz range), as well as bandwidths (6–23 Hz range), for the developed prototypes were determined. The maximal generated output power was almost 24 μW with a power density up to almost 600 μW/cm3.
Predictive Models for Elastic Bending Behavior of a Wood Composite Sandwich Panel
Authors: Mohammadabadi Mostafa , Jarvis James , Yadama Vikram , Cofer William
Published: June 2020

Strands produced from small-diameter timbers of lodgepole and ponderosa pine were used to fabricate a composite sandwich structure as a replacement for traditional building envelope materials, such as roofing. It is beneficial to develop models that are verified to predict the behavior of these sandwich structures under typical service loads. When used for building envelopes, these structural panels are subjected to bending due to wind, snow, live, and dead loads during their service life. The objective of this study was to develop a theoretical and a finite element (FE) model to evaluate the elastic bending behavior of the wood-strand composite sandwich panel with a biaxial corrugated core. The effect of shear deformation was shown to be negligible by applying two theoretical models, the Euler–Bernoulli and Timoshenko beam theories. Tensile tests were conducted to obtain the material properties as inputs into the models. Predicted bending stiffness of the sandwich panels using Euler-Bernoulli, Timoshenko, and FE models differed from the experimental results by 3.6%, 5.2%, and 6.5%, respectively. Using FE and theoretical models, a sensitivity analysis was conducted to explore the effect of change in bending stiffness due to intrinsic variation in material properties of the wood composite material.
Identification of Molecular Mechanisms Related to Pig Fatness at the Transcriptome and miRNAome Levels
Authors: Ropka-Molik Katarzyna , Pawlina-Tyszko Klaudia , Żukowski Kacper , Tyra Mirosław , Derebecka Natalia , Wesoły Joanna , Szmatoła Tomasz , Piórkowska Katarzyna
Published: June 2020

Fat deposition and growth rate are closely related to pork quality and fattening efficiency. The next-generation sequencing (NGS) approach for transcriptome and miRNAome massive parallel sequencing of adipocyte tissue was applied to search for a molecular network related to fat deposition in pigs. Pigs were represented by three breeds (Large White, Pietrain, and Hampshire) that varied in fat content within each breed. The obtained results allowed for the detection of significant enrichment of Gene Ontology (GO) terms and pathways associated directly and indirectly with fat deposition via regulation of fatty acid metabolism, fat cell differentiation, inflammatory response, and extracellular matrix (ECM) organization and disassembly. Moreover, the results showed that adipocyte tissue content strongly affected the expression of leptin and other genes related to a response to excessive feed intake. The findings indicated that modification of genes and miRNAs involved in ECM rearrangements can be essential during fat tissue growth and development in pigs. The identified molecular network within genes and miRNAs that were deregulated depending on the subcutaneous fat level are proposed as candidate factors determining adipogenesis, fatness, and selected fattening characteristics in pigs.
Preimplantation Genetic Testing for Chromosomal Abnormalities: Aneuploidy, Mosaicism, and Structural Rearrangements
Authors: Viotti Manuel
Published: June 2020

There is a high incidence of chromosomal abnormalities in early human embryos, whether they are generated by natural conception or by assisted reproductive technologies (ART). Cells with chromosomal copy number deviations or chromosome structural rearrangements can compromise the viability of embryos; much of the naturally low human fecundity as well as low success rates of ART can be ascribed to these cytogenetic defects. Chromosomal anomalies are also responsible for a large proportion of miscarriages and congenital disorders. There is therefore tremendous value in methods that identify embryos containing chromosomal abnormalities before intrauterine transfer to a patient being treated for infertility—the goal being the exclusion of affected embryos in order to improve clinical outcomes. This is the rationale behind preimplantation genetic testing for aneuploidy (PGT-A) and structural rearrangements (-SR). Contemporary methods are capable of much more than detecting whole chromosome abnormalities (e.g., monosomy/trisomy). Technical enhancements and increased resolution and sensitivity permit the identification of chromosomal mosaicism (embryos containing a mix of normal and abnormal cells), as well as the detection of sub-chromosomal abnormalities such as segmental deletions and duplications. Earlier approaches to screening for chromosomal abnormalities yielded a binary result of normal versus abnormal, but the new refinements in the system call for new categories, each with specific clinical outcomes and nuances for clinical management. This review intends to give an overview of PGT-A and -SR, emphasizing recent advances and areas of active development.
Association of Common Genetic Variants in the CPSF7 and SDHAF2 Genes with Canine Idiopathic Pulmonary Fibrosis in the West Highland White Terrier
Authors: Piras Ignazio S. , Bleul Christiane , Siniard Ashley , Wolfe Amanda J. , De Both Matthew D. , Hernandez Alvaro G. , Huentelman Matthew J.
Published: June 2020

Canine idiopathic pulmonary fibrosis (CIPF) is a chronic fibrotic lung disease that is observed at a higher frequency in the West Highland White Terrier dog breed (WHWT) and may have molecular pathological overlap with human lung fibrotic disease. We conducted a genome-wide association study (GWAS) in the WHWT using whole genome sequencing (WGS) to discover genetic variants associated with CIPF. Saliva-derived DNA samples were sequenced using the Riptide DNA library prep kit. After quality controls, 28 affected, 44 unaffected, and 1,843,695 informative single nucleotide polymorphisms (SNPs) were included in the GWAS. Data were analyzed both at the single SNP and gene levels using the GEMMA and GATES methods, respectively. We detected significant signals at the gene level in both the cleavage and polyadenylation specific factor 7 (CPSF7) and succinate dehydrogenase complex assembly factor 2 (SDHAF2) genes (adjusted p = 0.016 and 0.024, respectively), two overlapping genes located on chromosome 18. The top SNP for both genes was rs22669389; however, it did not reach genome-wide significance in the GWAS (adjusted p = 0.078). Our studies provide, for the first time, candidate loci for CIPF in the WHWT. CPSF7 was recently associated with lung adenocarcinoma, further highlighting the potential relevance of our results because IPF and lung cancer share several pathological mechanisms.
Assessment of 10-nm Particle Number (PN) Portable Emissions Measurement Systems (PEMS) for Future Regulations
Authors: Giechaskiel Barouch , Lähde Tero , Gandi Sawan , Keller Stefan , Kreutziger Philipp , Mamakos Athanasios
Published: June 2020

The particle number (PN) emissions of vehicles equipped with particulate filters are low. However, there are technologies that can have high PN levels, especially below the currently lower regulated particle size of 23 nm. Sub-23-nm particles are also considered at least as dangerous as the larger ultrafine particles. For this reason, the European Union (EU) is planning to regulate particles down to 10 nm. In this study we compared prototype portable emission measurement systems (PEMS) and reference laboratory systems measuring from 10 nm. The tests included cycles and constant speeds, using vehicles fuelled with diesel, gasoline or liquefied petroleum gas (LPG). The results showed that the PEMS were within ±40% of the reference systems connected to the tailpipe and the dilution tunnel. Based on the positive findings and the detection efficiencies of the prototype instruments, a proposal for the technical specifications for the future regulation was drafted.
Sex Differences in Body Composition Changes after Preseason Training in Elite Handball Players
Authors: Cichy Ireneusz , Dudkowski Andrzej , Kociuba Marek , Ignasiak Zofia , Sebastjan Anna , Kochan Katarzyna , Koziel Slawomir , Rokita Andrzej , Malina Robert M.
Published: June 2020

The purpose of this study was to evaluate changes in the estimated body composition of elite female and male Polish handball players during a five-week preseason training camp. Height and weight were measured, while body composition was estimated with bioelectrical impedance in 18 male and 17 female handball players before and after the five-week training protocol. Components of body composition included total body water (TBW), fat-free mass (FFM), muscle mass (MM), and absolute and relative fat mass (FM). Weight and body mass index (BMI) did not change in males, but declined in females after five weeks of training. FM and %FM declined, while estimated TBW, FFM, and MM increased significantly after training in both males and females. In contrast, comparisons of log transformed ratios for changes in weight, the BMI and body composition in males and females, respectively, suggested that estimated TBW, FFM, and MM increased relatively more in females than in males, while FM and %FM decline relatively more in males than females. Overall, the five-week preseason training program modified the body composition of male and female handball players. FM and %FM decreased, while estimated TBW, FFM, and MM increased, in both males and females after the preseason training program. Comparisons of log transformed ratios for changes in body composition in males and females suggested sexual dimorphism in response to intensive preseason training.
Adolescents’ Alcohol Use in Botellon and Attitudes towards Alcohol Use and Prevention Policies
Authors: Gervilla Elena , Quigg Zara , Duch Mariàngels , Juan Montse , Guimarães Clarisse
Published: June 2020

Alcohol is a common drug misused by young people worldwide. Previous studies have found that attitudes towards heavy consumption are stronger predictors than general norms concerning alcohol. This study aims to explore adolescents’ alcohol use and drunkenness, to understand adolescents’ attitudes towards alcohol use, drunkenness and prevention approaches, and to explore associations between attitudes and personal alcohol use and demographics. Methods: Cross-sectional face-to-face survey of 410 adolescents (61.2% women) who were socializing at night in the streets of Palma (Spain). Breath Alcohol Concentration (BrAC), self-reported measures of alcohol use and social variables were assessed. Results: 70.7% of respondents had a BrAC score higher than 0. The full sample reported having a mean of 3.9 drunk episodes in the last month, and a mean of 7.34 in Alcohol Use Disorders Identification Test (AUDIT). A total of 30.7% were under the minimum age limit for alcohol drinking in Spain and males showed higher BrAC than females. Bivariate analyses identified some differences in attitudes across participant demographics and personal alcohol use. In conclusion, we found high levels of alcohol use and drunkenness amongst adolescents, and adolescents’ attitudes towards drunkenness and prevention approaches were associated with their alcohol consumption as well as with age.
Biogenic Silica and Organic Carbon Records in Zhoushan Coastal Sea over the Past One Hundred Years and Their Environmental Indications
Authors: Xu Hao , Jiang Shangwei , Li Jialin , Pu Ruiliang , Wang Jia , Jin Wanghai , Sha Longbin , Li Dongling
Published: June 2020

The influence of terrestrial and marine input has dramatically changed eutrophication in coastal seas over the past 100 years. In this study, Zhoushan coastal sea (ZCS) is taken as a study area. We studied ZCS as it is a sink of the temporal and spatial variation of primary productivity, dominant species of algae, and the variation of provenance in this area over the past 100 years. We performed analysis using three sediment cores and the carbon and silicon deposition records. The analysis results demonstrate that: (1) The primary productivity in the northern area of the ZCS close to the Yangtze Estuary was the highest comparatively, but it declined slightly before 2010. The primary productivity in the southern area had an increasing trend over the past 100 years. The value of total organic carbon (TOC) in the northern area was relatively high, with an average value of 0.532% over the past 100 years, with a decreasing trend in recent years. On the contrary, TOC in the southern area was relatively low, but it was increased dramatically after 1995. (2) Diatom might play an important role in the variations. The biogenic silica (BSi) and TOC in the northern area showed a synchronous declining trend, while the BSi/TOC ratio did not change significantly. This indicates the algae population structure in this area was relatively stable over the past 100 years. The BSi/TOC ratio decreased continuously in the southern area, indicating that the dominance of diatoms was decreasing continuously. (3) The variation of diatom dominance in this area might have a great relationship with the change of nutrients’ provenance. A mean value of stable carbon isotope (δ13C) in the north of Zhoushan was −23.46‰, indicating that the terrestrial-source input was the highest. (4) The change of provenance in the study area was quite different. This illustrates that the terrestrial input impacted the largest area of ZCS while marine input became dominant in the offshore area.
Regional Differences in Prescribing Patterns of Metamizole in Germany Based on Data from 70 Million Persons
Authors: Hoffmann Falk , Bantel Carsten , von Rosen Frederik Tilmann , Jobski Kathrin
Published: June 2020

The non-opioid analgesic metamizole (dipyrone) is commonly used in Germany despite its narrow indications and market withdrawal from several countries. In this study we analyzed prescribing patterns of metamizole focusing on regional differences. The source of data was the “Information system for health care data” which includes data from the statutory health insurance funds for about 70 million Germans. We received aggregated data of individuals with at least one metamizole prescription in 2010 as well as the number of prescribed packages by age, sex, state and district along with the number of insured persons in each stratum. We calculated prescription prevalence stratified by age, sex, state and district. Among 68.4 million insured persons (mean age: 43.6 years; 53.0% female) 5.5 million received at least one metamizole prescription (8.1%, overall 12.2 million packages). Prevalence increased with age, and women received metamizole more often than men. In adults (total prevalence: 9.4%), levels varied between 7.0% (Saxony) and 11.1% (Schleswig-Holstein), whereas on a district level use ranged from 4.3% to 14.3%. In 2010, one of 12 individuals received metamizole at least once. Noticeable were the large regional variations which certainly cannot be explained by patient-related factors.
The Relationships between Maternal Feeding Practices and Food Neophobia and Picky Eating
Authors: Kutbi Hebah Alawi
Published: June 2020

Food neophobia and picky eating (FNPE) are dietary behaviors that have been frequently reported to coexist in children. Parental concerns about these dietary behaviors may influence the feeding practices employed. In this cross-sectional study, we investigated the bidirectional associations of maternal feeding practices with children’s FNPE behaviors. Using a convenience sampling technique, mothers of 195 healthy children aged 1–7 years were invited to complete a sociodemographic questionnaire, rate their child’s FNPE, and rate the extent to which each feeding practice was employed with the child. Maternal reports indicated that 37.4% (n = 73) of the children exhibited severe FNPE. Multiple linear regression analyses showed positive two-way associations between the “pressure to eat” feeding strategy and FNPE, and negative two-way associations between a healthy home food environment and FNPE. However, maternal practices of teaching and monitoring were not found to be associated with FNPE. Given the bidirectional relationships observed between FNPE and maternal feeding practices, primary health care providers should address the feeding practices used with a child and indicate that coercive feeding practices are counterproductive. Intervention studies targeting mothers of children with FNPE are needed to investigate whether specific maternal practices are more effective than others.
Evaluating Residential Segregation’s Relation to the Clustering of Poor Health across American Cities
Authors: Gibbons Joseph , Yang Tse-Chuan , Brault Elizabeth , Barton Michael
Published: June 2020

Residential segregation by race/ethnicity is widely recognized as a leading source of health disparities. Not clear from past research, however, is the overall health burden cities face due to clustering brought about by segregation. This study builds on previous research by directly measuring how spatially unequal health outcomes are within segregated cities. Utilizing Census-tract data from the Center for Disease Control and Prevention’s 500 Cities project, we examine how different dimensions of spatial segregation are associated with the clustering of poor self-rated health in cities. We make novel usage of the Global Moran’s I statistic to measure the spatial clustering of poor health within cities. We find spatial segregation is associated with poor health clustering, however the race/ethnicity and dimension of segregation matter. Our study contributes to existing research on segregation and health by unpacking the localized associations of residential segregation with poor health clustering in U.S. cities.
A Study on the Current Status and Improvement of the Digital Divide among Older People in Korea
Authors: Jun Woochun
Published: June 2020

In today’s knowledge- and information-based society, information literacy and information utilization skills are indicators of one’s competitiveness, and play a very important role in various fields (e.g., in one’s career, hobbies, as well as in daily life). In particular, information literacy and information utilization skills in older people are becoming essential for them to lead affluent lives. Moreover, information and communication technology is essential form of technology that can allow the elderly to ask for help in cases of emergency, as well as in daily life. Meanwhile, according to a recent Korean national statistical index, the digital divide among older people is more serious than that of the general public. The purpose of this paper is to statistically show that the digital divide among older people is more serious than other information-weak groups, as well as the general public. In addition, the purpose of this study is to identify the priorities that affect the digital divide among the three elements of the digital divide (digital access, digital capacity, and digital utilization) for older people. Based on that, we propose a variety of ways to solve the digital divide for older people. This study is expected to be widely used in future research and policies as a basis for solving the digital divide among older people.
Environmental Stimulation Counteracts the Suppressive Effects of Maternal High-Fructose Diet on Cell Proliferation and Neuronal Differentiation in the Dentate Gyrus of Adult Female Offspring via Histone Deacetylase 4
Authors: Liu Wen-Chung , Wu Chih-Wei , Hung Pi-Lien , Chan Julie Y. H. , Tain You-Lin , Fu Mu-Hui , Chen Lee-Wei , Liang Chih-Kuang , Hung Chun-Ying , Yu Hong-Ren , Chen I-Chun , Wu Kay L.H.
Published: June 2020

Maternal high-fructose diets (HFD) impair the learning and memory capacity of adult female offspring via histone deacetylase 4 (HDAC4). Hippocampal adult neurogenesis is important for supporting the function of existing neural circuits. In this study, we investigated the effects of maternal HFD on hippocampal neural stem cell (NSC) proliferation and neuronal differentiation in adult offspring. Increased nuclear HDAC4 enzyme activity was detected in the hippocampus of HFD female offspring. The Western blot analyses indicated that the expressions of sex-determining region Y box2 (SOX2) and the transcription factor Paired Box 6 (PAX6), which are critical for the progression of NSC proliferation and differentiation, were downregulated. Concurrently, the expression of Ki67 (a cellular marker for proliferation) and doublecortin (DCX), which are related to NSC division and neuronal differentiation, was suppressed. Intracerebroventricular infusion with class II HDAC inhibitor (Mc1568, 4 weeks) led to the upregulation of these proteins. Environmental stimulation reversed the expression of Ki67 and DCX and the counts of Ki67- and DCX-positive cells in the hippocampi of HFD offspring as a result of providing the enriched housing for 4 weeks. Together, these results demonstrate that the suppressive effects of maternal HFD on hippocampal NSC proliferation and neuronal differentiation are reversibly mediated through HDAC4 and can be effectively reversed by environmental stimulation. The advantageous effects of environmental enrichment were possibly mediated by HDAC4 suppression.
Emission-related Heavy Metal Associated with Oxidative Stress in Children: Effect of Antioxidant Intake
Authors: Killian Brittany , Yuan Tzu-Hsuen , Tsai Cheng-Hsien , Chiu Tina H. T. , Chen Yi-Hsuan , Chan Chang-Chuan
Published: June 2020

Heavy metals, the common pollutants emitted from industrial activities, are believed to cause harmful effects, partially through the mechanism of elevated oxidative stress, and antioxidant intake has been hypothesized to provide a potential protective effect against oxidative stress. This study aims to investigate the heavy metal exposure and the associated oxidative damage of young children living near a petrochemical complex and to assess the protective effect of antioxidant intake. There were 168 children recruited from the kindergartens near a huge petrochemical complex, with 87 as the high exposure group and 81 as the low exposure group. Urinary concentrations of eleven metals were detected by inductively coupled plasma mass spectrometry, and four biomarkers of oxidative stress were measured in urine by liquid chromatography-tandem mass spectrometry. The food frequency questionnaire was collected to assess participants’ intake of antioxidants. Multiple linear regression was performed to determine the predictors of metals for oxidative stress and to measure the beneficial effect of antioxidants. Weighted quantile sum regression was performed to determine the contributors among metals to the oxidative stress. Results showed that high exposure group had significantly higher concentrations of chromium, manganese, nickel, arsenic, strontium, cadmium, and lead when compared to those in low exposure group. There was no obviously difference on the total antioxidant intake and dietary profile between two groups. The elevated levels of two oxidative stress markers were significantly associated with most of the urinary metal concentrations in all study subjects after adjusting confounders, while no significant association was found between oxidative stress and antioxidant intake. Among the metals, mercury and strontium showed the dominated contributions for elevated levels of oxidative stress. It concluded that higher metal exposure was associated with elevated oxidative stress but with no protective effect by antioxidant intake among the young children residents near a petrochemical industry.
A Decision Method for Construction Safety Risk Management Based on Ontology and Improved CBR: Example of a Subway Project
Authors: Jiang Xiaoyan , Wang Sai , Wang Jie , Lyu Sainan , Skitmore Martin
Published: June 2020

Early decision-making and the prevention of construction safety risks are very important for the safety, quality, and cost of construction projects. In the field of construction safety risk management, in the face of a loose, chaotic, and huge information environments, how to design an efficient construction safety risk management decision support method has long been the focus of academic research. An effective approach to safety management is to structuralize safety risk knowledge, then identify and reuse it, and establish a scientific and systematic construction safety risk management decision system. Based on ontology and improved case-based reasoning (CBR) methods, this paper proposes a decision-making approach for construction safety risk management in which the reasoning process is improved by integrating a similarity algorithm and correlation algorithm. Compared to the traditional CBR approach in which only the similarity of information is considered, this method can avoid missing important correlated information by making inferences from multiple sources of information. Finally, the method is applied to the safety risks of subway construction for verification to show that the method is effective and easy to implement.
Early OA Stage Like Response Occurs after Dynamic Stretching of Human Synovial Fibroblasts
Authors: Nazet Ute , Grässel Susanne , Jantsch Jonathan , Proff Peter , Schröder Agnes , Kirschneck Christian
Published: June 2020

As events triggering early osteoarthritis onset can be related to mechanical stress and proinflammatory signaling, we investigated the effect of different mechanical strain protocols on the expression of proinflammatory genes, as well as extracellular matrix remodelling in human synovial fibroblasts. Three distinct models of tensile stretching were applied: static isotropic tensile strain at 0 Hz, 16% tension for 48 h; short-term high-frequency cyclic tension at 1 Hz, 10% tension for 4 h; and dynamic tensile stretching for 48 h, consisting of two blocks of moderate stretching at 0.2 Hz, 2%, advanced stretching at 0.5 Hz, 15%, or a combination of both. General signs of inflammation were present after static isotropic tension, whereas short-term high-frequency cyclic tension showed increased levels of IL-6 paired with diminished levels of IL-1β. Reduced inflammatory effects of TNF-α, IL-6, and IL-1β were observed when exposed to advanced stretching. Long-term tensile strain induced extracellular matrix remodelling at the gene and protein levels. While hyaluronan acid synthesis was increased with static tensile strain, dynamic tensile stretching had a reducing effect. Our study revealed that proinflammatory markers were activated by mechanical strain as seen in static isotropic tension and short-term high-frequency tensile strain, whereas long-term exposure induced extracellular matrix remodelling processes.
Production of Active Recombinant Hyaluronidase Inclusion Bodies from Apis mellifera in E. coli Bl21(DE3) and characterization by FT-IR Spectroscopy
Authors: Schwaighofer Andreas , Ablasser Sarah , Lux Laurin , Kopp Julian , Herwig Christoph , Spadiut Oliver , Lendl Bernhard , Slouka Christoph
Published: June 2020

The bacterium E. coli is one of the most important hosts for recombinant protein production. The benefits are high growth rates, inexpensive media, and high protein titers. However, complex proteins with high molecular weight and many disulfide bonds are expressed as inclusion bodies (IBs). In the last decade, the overall perception of these IBs being not functional proteins changed, as enzyme activity was found within IBs. Several applications for direct use of IBs are already reported in literature. While fluorescent proteins or protein tags are used for determination of IB activity to date, direct measurements of IB protein activity are scacre. The expression of recombinant hyaluronidase from Apis mellifera in E. coli BL21(DE3) was analyzed using a face centered design of experiment approach. Hyaluronidase is a hard to express protein and imposes a high metabolic burden to the host. Conditions giving a high specific IB titer were found at 25 °C at low specific substrate uptake rates and induction times of 2 to 4 h. The protein activity of hyaluronidase IBs was verified using (Fourier transform) FT-IR spectroscopy. Degradation of the substrate hyaluronan occurred at increased rates with higher IB concentrations. Active recombinant hyaluronidase IBs can be immediately used for direct degradation of hyaluronan without further down streaming steps. FT-IR spectroscopy was introduced as a method for tracking IB activity and showed differences in degradation behavior of hyaluronan dependent on the applied active IB concentration.
M1 Macrophages Promote TRAIL Expression in Adipose Tissue-Derived Stem Cells, Which Suppresses Colitis-Associated Colon Cancer by Increasing Apoptosis of CD133+ Cancer Stem Cells and Decreasing M2 Macrophage Population
Authors: Eom Young Woo , Akter Rokeya , Li Wanlu , Lee Suji , Hwang Soonjae , Kim Jiye , Cho Mee-Yon
Published: June 2020

We have previously reported that adipose tissue-derived stem cells (ASCs) cultured at high cell density can induce cancer cell death through the expression of type I interferons and tumor necrosis factor (TNF)-related apoptosis-inducing ligands (TRAIL). Here, we investigated whether TRAIL-expressing ASCs induced by M1 macrophages can alleviate colitis-associated cancer in an azoxymethane (AOM)/dextran sodium sulfate (DSS) animal model. M1 macrophages significantly increased the TRAIL expression in ASCs, which induced the apoptosis of LoVo cells in a TRAIL-dependent manner. However, CD133knockout LoVo cells, generated using the CRISPR-Cas9 gene-editing system, were resistant to TRAIL. In the AOM/DSS-induced colitis-associated cancer model, the intraperitoneal transplantation of TRAIL-expressing ASCs significantly suppressed colon cancer development. Moreover, immunohistochemical staining revealed a low CD133 expression in tumors from the AOM/DSS + ASCs group when compared with tumors from the untreated group. Additionally, the ASC treatment selectively reduced the number of M2 macrophages in tumoral (45.7 ± 4.2) and non-tumoral mucosa (30.3 ± 1.5) in AOM/DSS + ASCs-treated animals relative to those in the untreated group (tumor 71.7 ± 11.2, non-tumor 94.3 ± 12.5; p < 0.001). Thus, TRAIL-expressing ASCs are promising agents for anti-tumor therapy, particularly to alleviate colon cancer by inducing the apoptosis of CD133+ cancer stem cells and decreasing the M2 macrophage population.
Extracellular BMP Antagonists, Multifaceted Orchestrators in the Tumor and Its Microenvironment
Authors: Ouahoud Sarah , Hardwick James C.H. , Hawinkels Lukas J.A.C.
Published: June 2020

The bone morphogenetic proteins (BMPs), a subgroup of the transforming growth factor-β (TGF-β) superfamily, are involved in multiple biological processes such as embryonic development and maintenance of adult tissue homeostasis. The importance of a functional BMP pathway is underlined by various diseases, including cancer, which can arise as a consequence of dysregulated BMP signaling. Mutations in crucial elements of this signaling pathway, such as receptors, have been reported to disrupt BMP signaling. Next to that, aberrant expression of BMP antagonists could also contribute to abrogated signaling. In this review we set out to highlight how BMP antagonists affect not only the cancer cells, but also the other cells present in the microenvironment to influence cancer progression.
Expression and Role of Response Regulating, Biosynthetic and Degrading Genes for Cytokinin Signaling during Clubroot Disease Development
Authors: Laila Rawnak , Robin Arif Hasan Khan , Park Jong-In , Saha Gopal , Kim Hoy-Taek , Kayum Md. Abdul , Nou Ill-Sup
Published: June 2020

The obligate biotroph Plasmodiophora brassicae causes clubroot disease in oilseeds and vegetables of the Brassicaceae family, and cytokinins play a vital role in clubroot formation. In this study, we examined the expression patterns of 17 cytokinin-related genes involved in the biosynthesis, signaling, and degradation in Chinese cabbage inoculated with the Korean pathotype group 4 isolate of P. brassicae, Seosan. This isolate produced the most severe clubroot symptoms in Chinese cabbage cultivar “Bullam-3-ho” compared to three other Korean geographical isolates investigated. BrIPT1, a cytokinin biosynthesis gene, was induced on Day 1 and Day 28 in infected root tissues and the upregulation of this biosynthetic gene coincided with the higher expression of the response regulators BrRR1, on both Days and BrRR6 on Day 1 and 3. BrRR3 and 4 genes were also induced during gall enlargement on Day 35 in leaf tissues. The BrRR4 gene, which positively interact with phytochrome B, was consistently induced in leaf tissues on Day 1, 3, and 14 in the inoculated plants. The cytokinin degrading gene BrCKX3-6 were induced on Day 14, before gall initiation. BrCKX2,3,6 were induced until Day 28 and their expression was downregulated on Day 35. This insight improves our current understanding of the role of cytokinin signaling genes in clubroot disease development.
Gene Therapy for Cystic Fibrosis: Progress and Challenges of Genome Editing
Authors: Maule Giulia , Arosio Daniele , Cereseto Anna
Published: June 2020

Since the early days of its conceptualization and application, human gene transfer held the promise of a permanent solution to genetic diseases including cystic fibrosis (CF). This field went through alternated periods of enthusiasm and distrust. The development of refined technologies allowing site specific modification with programmable nucleases highly revived the gene therapy field. CRISPR nucleases and derived technologies tremendously facilitate genome manipulation offering diversified strategies to reverse mutations. Here we discuss the advancement of gene therapy, from therapeutic nucleic acids to genome editing techniques, designed to reverse genetic defects in CF. We provide a roadmap through technologies and strategies tailored to correct different types of mutations in the cystic fibrosis transmembrane regulator (CFTR) gene, and their applications for the development of experimental models valuable for the advancement of CF therapies.
Regulation of Wnt Signaling through Ubiquitination and Deubiquitination in Cancers
Authors: Park Hong-Beom , Kim Ju-Won , Baek Kwang-Hyun
Published: June 2020

The Wnt signaling pathway plays important roles in embryonic development, homeostatic processes, cell differentiation, cell polarity, cell proliferation, and cell migration via the β-catenin binding of Wnt target genes. Dysregulation of Wnt signaling is associated with various diseases such as cancer, aging, Alzheimer’s disease, metabolic disease, and pigmentation disorders. Numerous studies entailing the Wnt signaling pathway have been conducted for various cancers. Diverse signaling factors mediate the up- or down-regulation of Wnt signaling through post-translational modifications (PTMs), and aberrant regulation is associated with several different malignancies in humans. Of the numerous PTMs involved, most Wnt signaling factors are regulated by ubiquitination and deubiquitination. Ubiquitination by E3 ligase attaches ubiquitins to target proteins and usually induces proteasomal degradation of Wnt signaling factors such as β-catenin, Axin, GSK3, and Dvl. Conversely, deubiquitination induced by the deubiquitinating enzymes (DUBs) detaches the ubiquitins and modulates the stability of signaling factors. In this review, we discuss the effects of ubiquitination and deubiquitination on the Wnt signaling pathway, and the inhibitors of DUBs that can be applied for cancer therapeutic strategies.
CAR-T Cell Therapies: An Overview of Clinical Studies Supporting Their Approved Use against Acute Lymphoblastic Leukemia and Large B-Cell Lymphomas
Authors: Ahmad Aamir , Uddin Shahab , Steinhoff Martin
Published: June 2020

Chimeric Antigen Receptor (CAR)-T cell therapy is an exciting development in the field of cancer immunology, wherein immune T-cells from patients are collected, engineered to create ‘CAR’-T cells, and infused back into the same patient. Currently, two CAR-T-cell-based therapies, Tisagenlecleucel and Axicabtagene ciloleucel, are approved by FDA for the treatment of hematological malignancies, acute lymphoblastic leukemia and large B-cell lymphomas. Their approval has been a culmination of several phase I and II clinical studies, which are the subject of discussion in this review article. Over the years, CAR-T cells have evolved to be significantly more persistent in patients’ blood, resulting in a much-improved clinical response and disease remission. This is particularly significant given that the target patient populations of these therapies are those with relapsed and refractory disease who have often progressed on multiple therapies. Despite the promising clinical results, there are still several challenges that need to be addressed. Of particular note are the associated toxicities exemplified by cytokine release syndrome (CRS) and the neurotoxicity. CRS has been addressed by an FDA-approved therapy of its own—tocilizumab. This article focuses on the progress related to CAR-T therapy: the pertinent clinical studies and their major findings, their associated adverse effects, and future perspective.
A New Method for Collecting Large Amounts of Symbiotic Gastrodermal Cells from Octocorals
Authors: Chiu Hsiang-Yi , Lin Li-Yi , Chen Ying , Liu En-Ru , Li Hsing-Hui
Published: June 2020

The study of cnidarian-dinoflagellate endosymbiosis in octocorals is becoming increasingly important. As symbiotic gastrodermal cells (SGCs) are the key cells in a symbiotic relationship, obtaining SGCs and studying their functions represent an urgent need. The majority of the cells dissociated from octocoral tissues consist of host cells and algal cells, and very few intact SGCs can be observed. To solve this problem, we developed a new method to collect large amounts of SGCs from octocorals. We incubated the tissue of Sinularia flexibilis in high-salinity (60‰) filtered seawater for 6 h and were able to collect more than 18 times the number of SGCs from the control group. To test the quality of the dissociated cells, we performed three assays to evaluate their cell viability. All three assays demonstrated that cell viability was good after incubating in a high-salinity solution. We also used two other octocorals, Paralemnalia thyrsoides and Sinularia compressa, to perform the same experiment, and the results were similar to those for Sinularia flexibilis. Therefore, a high-salinity-induced increase in the SGC ratio is a common phenomenon among octocorals. This method allows researchers to collect large amounts of SGCs from octocorals and helps us to better understand the complex molecular interactions in cnidarian-dinoflagellate endosymbiosis.