bluuebunny/crossref_metadata_2025 huggingface dataset:
WITHDRAWN: Histopathological effect of ranitidine (Zantac) on liver and kidneys in albino mice
Authors: Rashid Muna Salah
Published: January 2023

The purpose: This study was sought to determine the effect of ranitidine on the histological structure of liver and kidneys in the albino mice. The design: two groups of mice were administered Ranitidine 75mg/kg of and 150mg/kg respectively, we used (15) albino mice and administrated the doses orally for 10 consecutive 10 days.
Finding : The results showed some structural effects on tissues of liver and kidneys group two showed (75mg/kg of b. w.) desquamation of the wall of central vein of the liver, necrosis , accumulation of fibroblast, hemorrhage and aggregation of Kupffer cells .the kidneys exhibited necrosis in some regions, hemorrhage and existence of cast in the tubules and it seems that ranitidine has caused dilation between glomerulus and bowman corpuscles. Group three (150mg/kg of b. w.) revealed more damage in the wall of the central vein of the liver, infiltration of focal inflammatory in the area caused hemorrhage ,expansion in sinusoid , accumulation of fibroblast and necrosis in the liver tissue. Kidneys were severely affected in comparison to group one, and showed an extended hemorrhage and ruptures in glomerulus with dilatation, hyperplasia cells in tubules and cast, form this study we conclude that ranitidine with a dose of 75mg/kg has mild effect on the liver and kidneys and medium effect with a dose of with a dose of 150 mg/kg.
Originality/ value: the research is focus in histopathology effects of ranitidine on liver and kidneys
NEK2 could act as a circadian time-dependent tumor suppressor gene and biomarker in esophageal squamous cell carcinoma
Authors: Zeng Boning , Li Nan , Sun Chao , Xie Shouxia , Liu Xiaodi , Peng Yuying , Wang Shaoxiang , Wang Xiao
Published: January 2023

Background
As one of the most prevalent cancers worldwide, esophageal squamous cell carcinoma (ESCC) is characterized by rapid progression and poor prognosis, thus, it is essential to develop novel biomarkers to evaluate the diagnosis and prognosis of ESCC patients. NIMA-related kinase 2 (NEK2) is an oncogenic kinase that has been found to be upregulated in a variety of cancers, and is considered a potential therapeutic target. In addition, circadian rhythms have been shown to be closely related to the development of cancer. However, the relationship between NEK2, circadian rhythm and ESCC progression remain unknown.
Methods
The Cancer Genome Atlas (TCGA), and Gene Expression Omnibus (GEO) databases (GSE23400 and GSE53625) were employed to collected the gene expression in ESCC patients, gene expression data, and clinical information. Several bioinformatics methods, including the Gene Set Enrichment Analysis (GSEA) and survival analysis were utilized to explore the correlation between the expression patterns of NEK2 genes and the development of ESCC. RNA sequencing was performed to investigate the expression of circadian rhythm. The effects of INH6 on the growth and proliferation of ESCC cell lines were tested in vitro by the Cell Counting Kit 8 (CCK8), 5-ethynyl-2ʹ-deoxyuridine (EdU), and colony formation. Additionally, western blotting was used to examined the expression of NEK2, cell cycle and apoptosis related protein, such as P21and cleaved PARP. reverse transcription-quantitative PCR (RT-qPCR) was employed to confirm that core circadian gene Bmal1, and Rev-Erba expressed. The cell apoptosis was detected by flow cytometry.
Results
Here, we first found that NEK2 gene was identified as differentially expressed genes in both the three datasets. Additionally, NEK2 is overexpressed in ESCC tissues and inhibition of NEK2 impairs ESCC cell growth and proliferation. The analysis of the KEGG and GSEA pathways revealed that NEK2 is closely related to known and recognized pathways such as the cell cycle, but an interesting new pathway related to NEK2 was also identified: circadian rhythm. RNA sequencing identified that NEK2 exhibits circadian rhythmic expression, and further in vitro studies confirmed that NEK2 has circadian oscillation in synchronized ESCC cells. More importantly, we observed that INH6 efficacy correlated positively with NEK2 expression levels in ESCC, suggesting that it is well worthwhile to develop and optimize the in vivo efficacy evaluation of NEK2 inhibitors based on chronological administration.
Conclusions
According to this study, NEK2 is highly expressed in human EC, and NEK2 has circadian oscillation in synchronized ESCC cells, which could be used to increase INH6 response to esophageal cancer therapy. In a word, NEK2 acts as a time-dependent tumor suppressor gene and biomarker in ESCC.
Degradation potential, use and growth promotion and antioxidant properties of Haematococcus Pluvialis in algae cell culture system under the influence of phenol
Authors: Rezazadeh Hamzeh , Mansouri Hakimeh
Published: January 2023

In response to highly severe environmental conditions, the accumulation of vast levels of astaxanthin by Haematococcus Pluvialis is a recorded event. However, there is little investigation into the nature and possible interactions of the poisonous chemical factors on this pigment's synthesis. In this study, H. pluvialis was exposed to the environment phenol to determine cellular responses, synthesis of pigments, and astaxanthin. The phenol influences in different concentrations (0, 50, 100, and 150 mg l-1) were checked out by two ways; a treatment (14 days) and pre-treatment (7 days + 7 days) done on green and red stages of H. pluvialis. The amount of carotenoid, protein, and dry weight increased, but the amount of carbohydrates and chlorophyll reduced in treated green cells with a phenol. The pre-treatment increased the amount of chlorophyll a, carbohydrates, and carotenoids in green cells, but it did not significantly affect the dry weight, protein of green cells, and the amount of protein in red cells. The phenol treatment also enhanced the carotenoid, carbohydrate, dry weight, and protein of red cells. In general, the treatment and pre-treatment of phenol in the green phase and low concentrations significantly affected the alga. However, the pre-treatment had a more considerable effect on red cells in high concentrations. While the treatment in the high concentrations had notable effects on the xanthophylls, pre-treatment had the same effects on the xanthophylls at low concentrations. These results show that H. pluvialis can adapt to toxic environments due to the xanthophylls.
Construction of a prognostic model for advanced non-small cell lung cancer using combined analysis of public databases
Authors: Yin Fangtian , Wu Min , Lu Xin , Zhu Jun , Wang Jiale , Zhang Xuewen , Chen Jie , Zheng Kaiman , Jiang Jun
Published: January 2023

Non-small cell lung cancer (NSCLC) has become a global health problem owing to its high incidence and poor treatment efficacy. This study aimed to establish a prognostic model for advanced NSCLC using The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) to evaluate the prognosis. Based on TCGA database, we obtained a dataset of 191 advanced NSCLC and 108 normal tissue samples after screening. We screened the differential expression between advanced NSCLC and normal tissues using the "limma" software package in R and identified differential expression genes. Weighted gene co-expression network analysis of differential genes was performed and the Gene Ontology database and Kyoto Encyclopedia of Genes and Genomes were used for pathway enrichment analysis of the key genes in the core modules. Finally, univariate Cox regression analysis was used to determine genes associated with prognosis, and multivariate Cox regression analysis was used to construct a prognostic model. We used the receiver operating characteristic curve (ROC), Kaplan-Meier curve, and multivariate Cox regression analysis to test the prognostic value of the model. The GEO database (GSE68465) was used to screen a cohort of clinical and gene expression data from 69 patients to further validate the prognostic value of the model. We constructed a five-gene (CKS1B, SLC7A5, SPATS2, SPDL1, and CHEK2) prognostic model to predict overall survival in patients with advanced NSCLC. Kaplan-Meier analysis, area under the ROC curve (AUC), and C-index had good predictive power for gene signatures in TCGA dataset (P<0.0001; C-index 0.710; 95% confidence interval (0.681–0.738); AUC at 1 year, 2 years, and 3 years were 0.76, 0.75, and 0.73, respectively). The risk score was an independent risk factor for survival in patients with advanced NSCLC, after adjusting for other routine clinical parameters. Finally, we constructed a nomogram that integrated gene signatures and clinicopathological parameters. The risk score model constructed from the five survival-related genes can be used as prognostic biomarkers. The nomogram was closely related to the overall survival of the patients. This result may help future individualized treatment and clinical decision making.
Association of maternal plasma manganese with the risk of spontaneous preterm birth: A nested case-control study based on the Beijing Birth Cohort Study (BBCS) in China
Authors: Han Weiling , Zheng Wei , Wang Aili , Chen Junxi , Wang Jia , Huang Junhua , An Hang , Ma Yuru , Zhang Kexin , Yang Ruihua , Yan Lailai , Li Zhiwen , Li Guanghui
Published: January 2023

Background
As an essential trace nutrient, the effect of plasma manganese (Mn) level on the risk of spontaneous preterm birth (SPB) remains debated. This study aimed to clarify the dynamic changes in plasma Mn concentration during pregnancy and its association with SPB.
Methods
A nested case-control study was conducted based on the Beijing Birth Cohort Study (BBCS).This cohort recruited women with a singleton pregnancy, aged 18-44, and intended to receive regular prenatal health care in Beijing Obstetrics and Gynecology Hospital between 2018 and 2020. 244 SPB with maternal plasma samples in the first or third trimester was selected as cases. 244 controls were randomly selected from full term birth mothers with plasma samples in the first or third trimester. Maternal social demographic characteristics and gestational information were obtained from clinical electrical records. Plasma samples were collected in the first and third trimesters for dynamic monitoring. Inductively coupled plasma mass spectrometry (ICP-MS) was used for laboratory analysis.Unconditional logistic regression was used to analyze the relationship between plasma Mn levels and SPB.
Results
A total of 488 pregnant women were included for final analysis (SPB N=244; term group N=244). Maternal plasma Mn con centrations increased with gestational age during pregnancy (median, 0.81 ng/ml in the first trimester vs. 1.23 ng/ml in the third trimester). After adjustment for confounders, the highest Mn level (3rdtertile) in the third trimester increased the SPB risk to 1.647(95%CI: 1.035-2.621).The association became much more significant when the population was stratified to normal weight women(OR: 2.066, 95%CI: 1.182-3.611) or non-premature rupture of membranes（PROM）women (OR: 3.929, 95% CI: 1.995-7.738).Moreover, a dose-dependent relationship exists between the SPB risk and plasma Mn concentration in non-PROMwomen (P trend＜0.001). After excluding women with vaginal group B streptococcus(GBS) infection, the risk association was still significant.
Conclusion
High plasma Mn level in the third trimester may be a risk factor for SPB, especially in women with normal weight and non-PROM. It would be helpful for SPB prevention to monitor Mn levels during pregnancy.
Trial registration
Beijing Birth Cohort Study (BBCS) was retrospectively registered (ChiCTR2200058395, 2022-04-08).
Outcomes Up to Age 36 Months after Congenital Zika Virus Infection – U.S. States
Authors: Neelam Varsha , Woodworth Kate R. , Chang Daniel J. , Roth Nicole M. , Reynolds Megan R. , Akosa Amanda , Carr Christopher P. , Anderson Kayla N. , Mulkey Sarah B. , DeBiasi Roberta L. , Biddle Cara , Lee Ellen H. , Elmore Amanda L. , Scotland Sarah J. , Sowunmi Similoluwa , Longcore Nicole D. , Ahmed Muhammad , Langlois Peter H. , Khuwaja Salma , Browne Shea Elizabeth , Lind Leah , Shim Kyoo , Gosciminski Michael , Blumenfeld Rachel , Khuntia Shreya , Halai Umme-Aiman , Locklear Autumn , Chan Mary , Willabus Teri' , Tonzel Julius , Marzec Natalie S. , Barreto Nianest Alers , Sanchez Connie , Fornoff Jane , Hale Shelby , Nance Amy , Adibhatla Sowmya N. , Potts Emily , Schiffman Elizabeth , Raman Devin , McDonald Morgan F. , Stricklin Brandi , Ludwig Elizabeth , Denson Lindsay , Contreras Dianna , Romitti Paul A. , Ferrell Emily , Marx Meghan , Signs Kimberly , Cook Amie , Beauregard Suzann , Orantes Lucia C. , Cronquist Laura , Roush Lesley , Godfred-Cato Shana , Gilboa Suzanne M. , Meaney-Delman Dana , Honein Margaret A. , Moore Cynthia A. , Tong Van T.
Published: January 2023

Background:
To characterize neurodevelopmental abnormalities in children up to 36 months of age with congenital Zika virus exposure.
Methods:
From the U.S. Zika Pregnancy and Infant Registry, a national surveillance system to monitor pregnancies with laboratory evidence of Zika virus infection, pregnancy outcomes and presence of Zika associated birth defects (ZBD) were reported among infants with available information. Neurologic sequelae and developmental delay were reported among children with ≥ 1 follow-up exam after 14 days of age or with ≥ 1 visit with development reported, respectively.
Results:
Among 2,248 infants, 9.9% were born preterm, and 10.0% were small-for-gestational age. Overall, 122 (5.4%) had any ZBD; 91.8% of infants had brain abnormalities or microcephaly, 23.0% had eye abnormalities, and 14.8% had both. Of 1,881 children ≥ 1 follow-up exam reported, neurologic sequelae were more common among children with ZBD (44.6%) vs. without ZBD (1.5%). Of children with ≥ 1 visit with development reported, 46.8% (51/109) of children with ZBD and 7.4% (129/1739) of children without ZBD had confirmed or possible developmental delay.
Conclusion:
Understanding the prevalence of developmental delays and healthcare needs these children with congenital Zika virus exposure can inform health systems and planning to ensure services are available for affected families.
User-centered design of a telehealth-enhanced hybrid cardiac rehabilitation program as hospital quality improvement
Authors: Duran Andrea T. , Keener-DeNoia Adrianna , Stavrolakes Kimberly , Fraser Adina , Blanco Luis V. , Fleisch Emily , Pieszchata Nicole , Cannone Diane , McKay Charles Keys , Whittman Emma , Edmondson Donald , Shelton Rachel C. , Moise Nathalie
Published: January 2023

Background Innovative program designs and strategies are needed to support the widespread uptake of cardiac rehabilitation (CR) programs in the post-COVID19 era. We combined user-centered design (UCD) and implementation science (ImS) principles to design a novel telehealth-enhanced hybrid (home and clinic-based) CR (THCR) program. Methods As part of a New York Presbyterian Hospital (NYPH) quality improvement initiative (March 2020-February 2022), we designed a THCR program using an iterative 3 step UCD process informed by the Theoretical Domains Framework and Consolidated Framework for Implementation Research to: 1) identify user and contextual barriers to CR uptake (stakeholder interviews), 2) design an intervention prototype (design workshops and journey mapping), and 3) refine the prototype (usability testing). The process was optimized for usability and implementation outcomes. Results Step 1: Semi-structured interviews with stakeholders (n = 9) at 3 geographically diverse academic medical centers revealed behavioral (e.g., self-efficacy, knowledge) and contextual (e.g., social distancing guidelines, physical space, staffing, reimbursement) barriers to uptake. Step 2: Design workshops (n = 20) and journey-mapping sessions (n = 3) with multi-disciplinary NYPH stakeholders (e.g., digital health team, CR clinicians, creative director) yielded a THCR prototype that leveraged NYPH’s investment in their remote patient monitoring (RPM) platform to optimize feasibility of home-based CR sessions. Step 3: Usability testing with CR clinicians (n = 2) administering and CR patients (n = 3) participating in home-based sessions revealed usability challenges (e.g., RPM devices/exercise equipment usability; Wi-Fi/Bluetooth connectivity/syncing; patient safety/knowledge and protocol flexibility). Design workshops (n = 24) and journey-mapping sessions (n = 3) yielded design solutions (e.g., onboarding sessions, safety surveys, fully supervised remote sessions) and a refined THCR prototype. Conclusion Combining UCD and ImS methods while engaging multi-disciplinary stakeholders in an iterative process yielded a theory-informed telehealth-enhanced hybrid CR program targeting user and contextual barriers to real-world CR implementation. We provide a detailed summary of the process, and guidance for incorporating UCD and ImS methods in early-stage intervention development. THCR may shrink the evidence-to-practice gap in CR implementation. A future hybrid type I effectiveness-implementation trial will determine its feasibility, acceptability, and effectiveness.
Distribution of number of genes in different bacteriophages
Authors: Ng Wenfa
Published: January 2023

Advent of low-cost, relatively fast DNA sequencing has opened up applications in genome sequencing. Besides benefiting sequencing of whole genomes of bacteria, archaea and fungi, it has also lend its weight to improve our understanding of bacteriophage genomes. Despite our vast amount of sequencing data, we still do not fully comprehend the generic principles that guide the evolution of genome architecture of the expansive number of bacteriophages in the microbial world. This work hopes to open a small glimpse into the mysteries surrounding the distribution of number of genes in the bacteriophage. Specifically, it attempts to construct a histogram distribution of number of genes in various bacteriophages in the hope of gleaning useful guiding principles that could help us better classify bacteriophages at the genome level. Using bacteriophage genome data obtained from GenBank, this work reveals that there is, in general, three main “size” classifications for bacteriophage genomes with genome size ranging from (43 to 143 genes), (143 to 243 genes) and (243 to 343 genes). Analysis in this study reveals that the smallest genome size is the most prevalent. Given the significant number of genes in the smallest bacteriophage genomes, there may be a need to rethink our understanding of bacteriophage, and whether they are the primitive microbial lifeforms that kickstarted the evolution of bacterial and, to a smaller extent, archaea life on Earth.
Enhancing the expression of a key mitochondrial enzyme at the inception of ischemia-reperfusion injury can boost recovery and halt the progression of acute kidney injury
Authors: Corridon Peter R.
Published: January 2023

Hydrodynamic fluid delivery has shown promise in influencing renal function in disease models. This technique provided pre-conditioned protection in acute injury models by upregulating the mitochondrial adaptation, while hydrodynamic injections of saline alone have improved microvascular perfusion. Accordingly, hydrodynamic mitochondrial gene delivery was applied to investigate the ability to halt progressive or persistent renal function impairment following episodes of ischemia-reperfusion injuries known to induce acute kidney injury (AKI). The rate of transgene expression was approximately 33% and 30% in rats with prerenal AKI that received treatments 1 (T1hr) and 24 (T24hr) hours after the injury was established, respectively. The resulting mitochondrial adaptation via exogenous IDH2 (isocitrate dehydrogenase 2 (NADP+) and mitochondrial) significantly blunted the effects of injury within 24 hours of administration: decreased serum creatinine (» 60%, p < 0.05 at T1hr; » 50%, p < 0.05 at T24hr) and blood urea nitrogen (»50%, p < 0.05 at T1hr; » 35%, p < 0.05 at T24hr) levels, and increased urine output (» 40%, p < 0.05 at T1hr; » 26%, p < 0.05 at T24hr) and mitochondrial membrane potential, Δψm, (» by a factor of 13, p < 0.001 at T1hr; » by a factor of 11, p < 0.001 at T24hr), despite elevated histology injury score (26%, p < 0.05 at T1hr; 47%, p < 0.05 at T24hr). Therefore, this study identifies an approach that can boost recovery and halt the progression of AKI at its inception.
DIAGNOSTIC VALUE OF MULTI-SLICE CT SCAN FOR RENAL ANGIO- MYOLIPOMA DETECTION: A CASE REPORT
Authors: Wenardi Kevin
Published: January 2023

Background: Angiomyolipoma (AML) is a rare solid benign tumor that occurs at many sites, more commonly in the kidney, which arises from either the renal pelvis or the sinus, also referred to as renal hamartoma. AML is composed of blood vessels, smooth muscle cells, and fat hence the name. They are seen 80-90% as isolated lesions that occur sporadically and seen 25-50% of patients with tuberous sclerosis. AML may hard to be distinguished from renal cell carcinoma because of its presentation as enlarged lump. Computed tomography may help to diagnose AML. To get a definite diagnosis of AML, excision and histopathological examination is recommended in symptomatic patients, especially to prevent rupture hemorrhage. Objective: The purpose of this study was to evaluate multi-slice computed tomography with contrast in a male patient aged 49 years with Angiomyolipoma Case description: We herein present a case of renal AML in a 49 years old male patient, who presented with left flank pain for ~1 year. He has no history of hematuria and dysuria and tuberous sclerosis. Multi-slice computed tomography with contrast demonstrates a hypodense inhomogenous mass measuring 11x9,3 cm in the upper pole of the left kidney, hypertrophy of the left kidney, and hump sign of left kidney. At the later date, the patient underwent total left nephrectomy at another hospital and the postoperative histopathological examination confirmed the lesion as an AML. Conclusion: Angiomyolipoma is a rare benign tumor which usually occurs in the kidney that can be confused with malignant tumor. Ultrasonography and computed tomography can help to diagnose AML. The symptomatic patient requires surgical intervention.
PEDIATRIC HEALTHCARE SERVICE FOR AS A PART OF INDONESIAN FUTURE
Authors: Silalahi Lothar Matheus Manson Vanende
Published: January 2023

Continuing and accelerating child health are issues stated in Sustainable Development Goals (SDG) by United Nation (UN). Targets in improving child health in SDG are by decreasing neonatal and pediatric mortality. Reducing risk and prevention of neonatal mortality may be achieved by monitoring intrauterine fetal development. High burden of pediatric mortality may be seen in pediatric genetic, neoplasm and infectious disease. In this COVID-19 pandemic era, SARS-COV2 infection in children add more challenge in improving child health. In this issue, we proudly present articles on pediatric genetic, neoplasm and infectious disease especially in clinical manifestation, diagnostic and therapeutic challenge.
CARA MUDAH MERAWAT LUKA (AN EASY WAY TO CARE FOR WOUNDS)
Authors: Prasetyo Yacobus Christian
Published: January 2023

Merupakan resensi buku Cara Mudah Merawat Luka.
The Comparisons of Lavender and Jasmine Aromatherapy Effectiveness on Depression Level for Medical Students
Authors: Sukarman Tania Lysandra , Gani Irna Permanasari , Jasaputra Diana Krisanti
Published: January 2023

Background Everyone can suffer from depression, including teenagers. Students in the medical faculty show higher depression levels than other faculties students due to being unable to adapt to the new daily activity as medical students. Lavender and Jasmine aromatherapy can reduce depression levels because it contains linalool.Objective The purpose of this research is to compare the effectiveness between Lavender and Jasmine aromatherapy on Maranatha Christian University medical student depression levels.Method This analytical research design used a simple random sampling method on 45 subjects divided into 3 groups: Lavender, Jasmine, and Control. The data measured was the total score of the BDI II (Beck Depression Inventory II) questionnaire before and after being given treatment for 1 week.Results The average BDI II score in the Lavender and Jasmineâ€™s groups decrease from 19.73 (moderate depression) to 14.73 (mild depression) in the Lavenderâ€™s group and 19,33 (moderate depression) to 14.06 (mild depression) in Jasmineâ€™s. Thereâ€™s also decreased score in Control group, from 20.60 to 18.86 (mild depression).Â  Furthermore, data is converted into BDI IIâ€™s scores decrease percentage, 24.00% for Lavenderâ€™s group, 28.85% for Jasmineâ€™s, and 5.80% for Controlâ€™s. Data were tested by the Kruskal Wallis test with significant results p=0.001 (p<0.05) followed by Mann-Whitney test.Conclusion Resume of this research is both Lavender and Jasmine aromatherapy has equal potential for reducing depression levels.Conclusion Resume of this research is both Lavender and Jasmine aromatherapy has equal potential for reducing depression levels.
Medical Clerkshipsâ€™ Perception and Factors in Acquisition of Knowledge and Skills During Covid-19 Pandemic
Authors: Purnajati Oscar Gilang , Widowati Fransisca Galuh , Kristianto Haryo Dimasto
Published: January 2023

Background The global COVID-19 pandemic has changed Medical Education in general. Many changing policies are a challenge, and we must evaluate the results. Medical clerkship program presents a challenge of its own. Delay in rotation time, work restrictions, and reduced contact with patients can cause a lack of accuracy and realism in integrating knowledge and skills that should be the outcome of this clerkship.Objective We need to explore the impact of COVID-19 pandemic in clinical rotation in general perception and the factors that influence the acquisition of knowledge and skills in medical clerkship education.Method A qualitative study by comparing 2 groups, before pandemic and during pandemic group and explores the factors that influence the acquisition of knowledge and skills through focus group discussions (FGD).Results Conclusion The general perception obtained in this study included workload and knowledge acquisition, and the specific factors that influenced the acquisition of knowledge and skills were the existence of role models, feedback, and teaching and learning models during the pandemic.Conclusion The COVID-19 pandemic did not significantly affect knowledge acquisition of medical clerkship, but it had some effect on skill acquisition. Specific factors that influenced medical clerkship learning during this pandemic were role modelling and teaching-learning because of reduced face-to-face session between the clinical supervisors and the student. The feedback factor was almost not affected.
INTERNAL FACTORS AFFECTING ONLINE LEARNING ACCORDING TO STUDENT REFLECTION
Authors: Hapsari Saverina Nungky Dian , Vanadian Heppi
Published: January 2023

Background The COVID-19 pandemic has changed many aspects of life worldwide, including medical education. Offline learning is being replaced by online to reduce the spread of disease through direct contact between the academic community. Along with technological developments, online learning is likely to continue even after the COVID-19 pandemic is over. Therefore, students must be able to adapt and prepare for the next online learning. The preparation can be started by self-assessing the online learning that has been followed so far and finding things that must be improved in future online learning.Objective To explore the studentâ€™s performance during online learning.Methods This research was qualitative research using the reflection writings of 156 students regarding their performance during online learning, which would be analyzed according to the depth and content of the reflection.Results Internal factors that support (strengths) and internal factors that inhibit online learning (weaknesses) through student reflection during online learning were found in this study. Internal factors such as maintaining health, self-management, adapting, and maintaining motivation can be maintained to support online learning. Internal factors such as anxiety, avoiding interaction, laziness, failure in time management, and inappropriate learning methods hindered online learning.Conclusion Students can strengthen internal factors that support online learning and reduce internal factors that hinder online learning to optimize online learning in the future.
EDITORIAL Role of Obesity in Breast Cancer Metastasis and Relapse
Authors: Jayadi Tejo
Published: January 2023

The breast cancer patients survivors expected to be increased, because of better early cancer detection and better treatment. Although the survivorship should be celebrated, but there are common late effect of cancer and its treatment. So, it need to be better health care treatment provided to the survivor to give them better quality of life.
Çerkeslerde Vatana Dönüş Sürecinin Uzun Öyküsü
Authors: KURMEL Ömer Aytek
Published: January 2023

Sovyetler Birliğinin 1943 yılı gibi erken bir tarihte Batı’daki Sovyet muhaceretini geri getirmeyi resmi politika haline getirmiş olmasına karşın, Çerkes diasporası Kafkasya’ya dönüş düşüncesini ancak 1960’lı yılların ortasında, üstelik kendisini merkeze alarak tartışmaya başladı. Moskova, savaş sonunda Batı ülkelerinde kalan kalabalık Sovyet muhaceretini kendi güvenliği için bir tehdit olarak algılıyor ve geri getirmek istiyordu. Stalin’in ölümünden sonra ayrıntılı bir göçmen politikası oluşturan Kremlin, sorumluluğu farklı biçimde dağıtarak değişik göçmen kategorileri kurguladı. Dönüş kampanyası Güney Amerika’dan dönen birkaç yüz kişi dışında başarılı olmasa da göçmen toplulukları içinde güvensizlik tohumları ekmeyi başardı. Çerkesler düzleminde, Türkiye dışındaki ülkelerde yaşayan Çerkesler dönüş konusuna daha yapıcı yaklaştılar. NATO üyesi Türkiye’deki Çerkeslerin tutucu kesimleri dönüş kampanyasını Sovyet komplosu olarak şeytanlaştırdılar. Dönüş tezini soğukkanlılıkla tartışmayı beceremeyen Türkiye Çerkesleri, etkisi bugün bile hissedilecek biçimde Suriyeli ve Ürdünlü soydaşlarının gerisinde kaldılar
Impact of laptop dealer firm’s service quality on customers’ loyalty in Jordan
Authors: M. Abu Hussien Al-Harith , Al Saed Rashad , A. I. Shajrawi Ahmad , Albloush Ahmad , Ali Hussam
Published: January 2023

This study aims to investigate how various aspects of service quality in laptop dealer companies affect customers’ loyalty in Jordan. Data were collected from 341 Jordanian clients of dealer firms selling laptops in Jordan (HP, LG, Toshiba, Apple, Samsung, Sony, and Dell). Collected data were then analyzed; the hypotheses were assessed with the help of SPSS and structural equation modeling. The findings showed that customers’ loyalty is significantly impacted by reliability, accessibility to service, and safety. Furthermore, the value of beta represents relative importance. The dimension of safety showed the highest level in size of the impact on customers’ loyalty, and its value was 0.446 of the total impact. Next, the access to service dimension was the second factor in the impact size (0.239), and the reliability dimension demonstrated the smallest impact size with 0.146. Additionally, the findings revealed that reliability, access to service, and safety explain 52% of the variance in customers’ loyalty to purchase a laptop in Jordan. As a result, the study recommends that Jordanian laptop dealer firms consider all three service quality characteristics, particularly safety, to maintain the loyalty of their customers.
Secondary interpretation of scientific knowledge in mass media discourse
Authors:   Derzhavin Tambov State University, Boldyrev Nikolay N. , Efimenko Tatiana N. ,   Derzhavin Tambov State University
Published: January 2023

The article considers the problem of science representation in media discourse within the framework of cognitive approach and the theory of language interpretation. The object of research encompasses secondary scientific texts: critical reviews, reviews, abstracts presented on the educational and scientific organizations websites, and scientific news releases on sites aimed at net coverage. The research focuses on identifying main factors that determine the interpretative specificity of scientific knowledge in media discourse, with respect to the conceptual nature of secondary language interpretation. The authors argue that distinctive features of scientific knowledge conceptual representation in media highly depend on the basic principles of conceptual interaction between the author of the message and the target audience, as well as on the cognitive processes and mechanisms of meaning construction specific to the secondary language interpretation: the process of conceptual derivation and profiling mechanisms — generalization and specialization. They draw attention to the distinction between messages addressing professional or mass audience in terms of level and volume of transmitted scientific knowledge. Language specificity of its secondary interpretation shows up in the use of basic or special terminology, language derivation models, and types of organization and genre features of discourse. The results and conclusions drawn are substantiated by the analysis of main types and functions of secondary language interpretation: selective, classifying, evaluative, as illustrated by messages from different subject domains. The authors come to the conclusion that degree of effectiveness of conceptual interaction between the author of the message and the target audience in the processes of transmitting and interpreting scientific knowledge largely depends on the alignment in these types and functions of secondary language interpretation applied on both author and audience sides. The latter (especially with respect to the shared knowledge in a particular field) also determines the degree of conflictfree scientific media communication in general.
Visualization in medical advertising discourse in Germany
Authors:   Bashkir State University, Tayupova Olga I. , Poliakova Ekaterina V. ,   Bashkir State University
Published: January 2023

A study was made of non-verbal signs used to visualize the printed text of advertising, selected both by belonging to the corresponding subdiscourse and taking into account the territorial feature. Advertising as a type of media text, which has a special composition and influences consumer behavior with the help of specific means, consists of two interrelated codes: a verbal code and a visual code. It has been established that the non-verbal code of this type of text not only transmits information about over-the-counter drugs in order to maintain and enhance sales, but also contains information about the cultural situation of the country, about ethnocultural stereotypes and values prevailing in German society, contributing, in turn, their promotion in society. The authors, setting themselves the goal of identifying the visual resources involved in the creolized advertising texts of leading German pharmaceutical companies, attempt to systematize the idea of the visualization tools used here, correlating with gender parameters, national and cultural characteristics of Germany, its national identity, taking into account technological solutions in the colour picker. The suggestive potential of visual means that affect the potential consumer of over-the-counter medicines due to the appeal to what is close and understandable to him as a representative of a certain cultural community or gender has been determined. It was revealed that in the visual plan, advertising of over-the-counter drugs not only adapts to the cultural environment, reflecting the originality of the national-specific picture of the world of the Germans, their mentality and preferred lifestyle but also reveals a close connection with the symbols of medicine. Cultural signs, directly related to the cultural and value space of Germany, are a universal tool for influencing potential consumers of over-the-counter medicines.
Updating means metaprograms in the titles of audiovisual mediacontent in popular science sector of video blogging
Authors:   Voronezh State University, Shesterina Alla M.
Published: January 2023

Trends in mediatization of various spheres of our life, accompanied by an increase in audience interest in video content, make the problem of studying the latter from the standpoint of the effectiveness of its participation in the process of internalization of scientific knowledge relevant. This process is complicated by such features of the network environment as the low level of professionalism of a number of authors, the active process of spreading pseudoscientific information, and the highly competitive nature of relations between bloggers in the network space. The first two problems are being overcome by bloggers as researchers note the professionalization of the blogosphere. The last problem is solved by the authors themselves inside the materials. To solve it, bloggers often resort to updating the key meta-programs that dominate the modern media field. The article analyzes the verbal means of emphasizing these programs in the Russian-language sector of popular science video blogging. Based on the analysis of the verbal level of the releases of the most popular YouTube channels, a conclusion is made about the frequency of occurrence of each of these meta-programs in the structure of the media text. The fact of the dependence of the degree of popularity of the metaprogram on the scientific sphere, which is the subject of the narration, is established. The conclusion is made about the dominance of the metaprogram “values” as the most appropriate for this sector of media. It is concluded that in a situation where scrolling is the basis of the user’s information behavior on the network, it is the titles of the videos (along with the preview) that stimulate the viewer’s interest, and metaprograms play an important role in this process.
Pragmalinguistic approach in the ambivalence idea in term of COVID-19 laughter culture
Authors:   Rostov State Transport University, Taktarova Anna V. , Cherkasova Marina N. ,   Rostov State Transport University
Published: January 2023

Relevance of our research is determined by the practical results of three scientific analyzes. The first one has showed Top-10 discussed problems on Internet with the key words “modern system” (with “Modern system of education” on the Runet’s top). The second one is a survey of Internet communication. It demonstrates humorous component in media-texts on a given theme. The third one is the main pragmalinguistic analysis. It deals with the media-texts with a humorous component from the ambivalent point of view. Empirical base of our paper is the media-texts with humorous component. They are devoted to the educational process in Russian Federation in 2020. The texts were analyzed with conative oriented strategies “Author`s expectation” and “Author`s assessment. The expectation category is connected with “accepted” or “facial reality” of the media-text`s sender; assessment category reflected positive-approving, negative-disapproving and neuter- indifferent assessment contains a certain affective reaction on situation. The strategies are textual and regulatory simultaneously. They may be applied not only according to the indicator`s direction to the media text`s receiver, but according to facial, social and idiographic characteristics of the text`s author. The subject of the research is the author’s choice of those variants of the statement which are preferable for material presentation and for latent regulation of the receiver. SSG (smaller scale syntax group) is the unit of our analysis. The sender of the text is obligatory actualized definite grammar form in this group. Ambivalent approach “assessment vs reaction” — “assessment vs reality” to media area in terms of laughter culture has revealed mixed feelings of the media-text`s senders to the modern system of education and it is a part of mass consciousness. Media-texts with humor effect create the ambivalent part of mass consciousness as a common experience and response to crisis of Russian Federation educational system.
Curcumin Combats against Gastrointestinal Cancer: A Review of
Current Knowledge Regarding Epigenetics Mechanisms with a
Focus on DNA Methylation
Authors: Jafari-Nozad Amir Masoud , Jafari Amirsajad , Zangooie Alireza , Behdadfard Mohammad , Zangouei Amir Sadra , Aschner Michael , Farkhondeh Tahereh , Samarghandian Saeed
Published: January 2023

Gastrointestinal (GI) cancers are one of the most common human malignancies
and a leading cause of morbidity and mortality worldwide. One of the most prominent
hallmarks of cancer and a basic trait of almost all GI malignancies is genomic/epigenomics
alterations. DNA methylation is highlighted as a fundamental mechanism underlying
the inactivation of several tumor-suppressor gene signaling pathways. Thus, sites
of DNA methylation can be triggered for cancer therapy. Available therapeutic procedures
for GI cancer show unsatisfactory efficacy, and some treatments are associated
with severe side effects, including ulceration or bleeding. Therefore, it is essential to find
alternative treatments. There is growing evidence indicating that some chemopreventive
phytochemicals can combat cancer. One of the most systematically investigated nutraceuticals
for its advantages in managing different diseases is curcumin (CUR). CUR is well
known for its potent anticancer characteristics by targeting epigenetic mechanisms, with
DNA methylation at the forefront. Prior investigations have indicated that CUR treatment
can benefit GI cancers by controlling several signaling pathways related to oxidative
stress and epigenomics pathways. The present literature displays recent evidence regarding
DNA methylation alterations by CUR and its potential role in GI cancer prevention
and treatment.
Intracellular Ellagic Acid Derived from Goat Urine DMSO Fraction
(GUDF) Predicted as an Inhibitor of c-Raf Kinase
Authors: Raj Ajay Kumar , Lokhande Kiran Bharat , Prasad Tanay Kondapally , Nandangiri Rasika , Choudhary Sumitra , Pal Jayanta Kumar , Sharma Nilesh Kumar
Published: January 2023

Background:

Dietary chemicals and their gut-metabolized products are
explored for their anti-proliferative and pro-cell death effects. Dietary and metabolized
chemicals are different from ruminants such as goats over humans.

Methods:

Loss of cell viability and induction of death due to goat urine DMSO fraction
(GUDF) derived chemicals were assessed by routine in vitro assays upon MCF-7
breast cancer cells. Intracellular metabolite profiling of MCF-7 cells treated with goat
urine DMSO fraction (GUDF) was performed using an in-house designed vertical tube
gel electrophoresis (VTGE) assisted methodology, followed by LC-HRMS. Next, identified
intracellular dietary chemicals such as ellagic acid were evaluated for their
inhibitory effects against transducers of the c-Raf signaling pathway employing
molecular docking and molecular dynamics (MD) simulation.

Results:

GUDF treatment upon MCF-7 cells displayed significant loss of cell viability
and induction of cell death. A set of dietary and metabolized chemicals in the intracellular
compartment of MCF-7 cells, such as ellagic acid, 2-hydroxymyristic acid, artelinic
acid, 10-amino-decanoic acid, nervonic acid, 2,4-dimethyl-2-eicosenoic acid, 2,3,4'-
Trihydroxy,4-Methoxybenzophenone and 9-amino-nonanoic acid were identified.
Among intracellular dietary chemicals, ellagic acid displayed a strong inhibitory affinity
(-8.7 kcal/mol) against c-Raf kinase. The inhibitory potential of ellagic acid was found
to be significantly comparable with a known c-Raf kinase inhibitor sorafenib with overlapping
inhibitory site residues (ARG450, GLU425, TRP423, VA403).

Conclusion:

Intracellular dietary-derived chemicals such as ellagic acid are suggested
for the induction of cell death in MCF-7 cells. Ellagic acid is predicted as an inhibitor
of c-Raf kinase and could be explored as an anti-cancer drug.
Design, Synthesis, and Biological Evaluation of a Class of TEMPOmodified Naphthalimide Derivatives with Good Selectivity for Tumor Cells
Authors: Hao Qingqing , Zhang Yujiao , Jiang Xuliang
Published: January 2023

Background:

Naphthalimides have been receiving continuous attention for their outstanding
performance in treating cancer. However, as a class of cytotoxic anti-tumor agents, their toxic side effects
greatly limit their clinical application. Therefore, developing naphthalimide reagents with good selectivity
for tumor cells is of great importance.

Methods:

A class of TEMPO-modified naphthalimide derivatives substituted with oxygen at the 6-
position were designed, synthesized, and evaluated for the anti-tumor activity in vitro against three cancer
cell lines (A549, MCF-7, and HeLa) as well as one normal human liver cell line (QSG-7701) by MTT
assay. HRMS (or MS) and EPR spectra were used to identify the structures of target compounds. The
UV–vis titration absorption spectra were performed to study the interaction between compounds and
DNA.

Results:

The target compounds NT3, NT9, NT10, NT11, and NT12 exhibited moderate inhibition to all
the three tested tumor cell lines, while NT1, NT2, and NT4 showed selectivity against A549, All the target
compounds did not show measurable activities in QSG-7701, which imply the target compounds are
likely to overcome the nonspecific toxicity against normal cells. UV-vis spectra showed this class of
compounds could effectively intercalate into DNA and selectively bind to G-C base pairs.

Conclusion:

A novel class of TEMPO-modified naphthalimide derivatives substituted with oxygen at 6-
position and the preliminary discussion on their SARs provide promising opportunities to guide further
research on naphthalimide derivatives as anti-tumor agents.
Das Fugenelement -e- in NN-Komposita im Mooringer Friesisch als Stammerweiterung
Authors: Handke V. , Hoekstra J. , Rohlf N.
Published: January 2023

In this article we discuss the distribution of the linking element -e- in NN-compounds in Mooring Frisian, a mainland North Frisian dialect. Although this linking element is homophonous with the regular plural ending -e in Mooring Frisian and triggers plural allomorphy, we argue against an equation of linking element and plural ending. We propose that linking elements are semantically void augments or stem extensions, that may optionally serve phonological (prosodic) and/or quasi-morphological functions. Thus the linking element -e- can build trochees and function as an indirect marker of the stem inherent morphosemantic features (countability, animacy) or the first component’s contextual meaning (plural interpre¬tation). In addition, we address some dialectological and historical aspects of the development of the linking element -e- in Mooring Frisian in Mooring Frisian.
Strategies for automotive filter component manufacturers towards electric vehicles era in Indonesia
Authors: Nurcahyo Rahmat , Nurhadi Hikari , Habiburrahman Muhammad
Published: January 2023

In conventional cars, a filter is one of the automotive components directly associated to the internal combustion engine (ICE) and will be disappeared if electric vehicles dominate. By carrying out a case study approach to filter manufacturers and formulating problems with a strategic management approach and SWOT analysis, the results show that filter manufacturers can still take advantage of opportunities by using their existing internal strengths; therefore, an alternative strategy is to implement aggressive or diversification strategies in the future.