bluuebunny/crossref_metadata_2025 huggingface dataset:
Data from Netrin-1 Promotes the Immunosuppressive Activity of MDSCs in Colorectal Cancer
Authors: Xia Xueli , Mao Zhenwei , Wang Wenxin , Ma Jie , Tian Jie , Wang Shengjun , Yin Kai
Published: May 2023

Abstract
Myeloid-derived suppressive cells (MDSC) inhibit antitumor immunity and confer a survival advantage for tumor evasion. Tumor cells also support MDSC expansion and recruitment by secreting multiple growth factors and cytokines, but the mechanisms by which tumors affect MDSC function are not completely understood. Here, we found that the neuronal guidance protein netrin-1 was selectively secreted by MC38 murine colon cancer cells, which could enhance the immunosuppressive activity of MDSCs. MDSCs predominantly expressed one type of netrin-1 receptor, adenosine receptor 2B (A2BR). Netrin-1 interacted with A2BR on MDSCs to activate the cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA) signaling pathway, which ultimately increased CREB phosphorylation in MDSCs. Furthermore, netrin-1 knockdown in tumor cells inhibited the immunosuppressive activity of MDSCs and restored antitumor immunity in MC38 tumor xenograft mice. Intriguingly, high netrin-1 in the plasma correlated with MDSCs in patients with colorectal cancer. In conclusion, netrin-1 significantly enhanced the immunosuppressive function of MDSCs through A2BR on MDSCs, thus promoting the development of tumors. These findings highlight that netrin-1 may regulate the abnormal immune response in colorectal cancer and may become a potential target for immunotherapy.
Supplementary Tables S1-S6 from Ultra High-plex Spatial Proteogenomic Investigation of Giant Cell Glioblastoma Multiforme Immune Infiltrates Reveals Distinct Protein and RNA Expression Profiles
Authors: Bonnett Shilah A. , Rosenbloom Alyssa B. , Ong Giang T. , Conner Mark , Rininger Aric B.E. , Newhouse Daniel , New Felicia , Phan Chi Q. , Ilcisin Saskia , Sato Hiromi , Lyssand John S. , Geiss Gary , Beechem Joseph M.
Published: May 2023

Supplementary Table S1: A list of materials and reagents that are recommended for the GeoMx DSP assays that are not supplied by NanoString. Supplementary Table S2. A list of NanoString supplied materials and reagents. Supplementary Table S3: A list of Human protein targets by module. Supplementary Table S4: A list of Mouse protein targets by module. Supplementary Table S5: The 11-cell pellet array (CPA) used in assay development. Supplementary Table S6: The 45-cell pellet array (CPA) used in assay development.
Supplementary Figures S19-S22 from Ultra High-plex Spatial Proteogenomic Investigation of Giant Cell Glioblastoma Multiforme Immune Infiltrates Reveals Distinct Protein and RNA Expression Profiles
Authors: Bonnett Shilah A. , Rosenbloom Alyssa B. , Ong Giang T. , Conner Mark , Rininger Aric B.E. , Newhouse Daniel , New Felicia , Phan Chi Q. , Ilcisin Saskia , Sato Hiromi , Lyssand John S. , Geiss Gary , Beechem Joseph M.
Published: May 2023

Supplementary Figure S19: Representative images used in the assessment of spatial proteogenomic data quality on Glioblastoma multiforme Grade 4. (A) Human brain glioblastoma tissue array was stained with the GeoMx NGS Human Protein modules (147-plex), WTA, and antibodies against GFAP (Astrocyte/Tumor; left panel), CD45 (Immune; middle panel) and Syto13 (Nuclei; right panel). (B) ROI were segmented into GFAP+ (left panel), CD45+ (middle panel) or Syto13 (right panel).

Supplementary Figure S20: Representative images used in the assessment of spatial proteogenomic data quality on gcGBM and GBM. ROIs for gcGBM (top panel) and GBM (bottom panel) were segmented into GFAP+ (Astrocyte/Tumor; left panel), CD45+ (Immune; middle panel) and Syto13 (Nuclear, DNA; right panel).

Supplementary Figure S21: Combined volcano plot resulting from the differential expression analysis between GBM and gcGBM for all AOIs within the left frontal lobe for RNA (●) and protein (♦) analytes. Targets with significantly differential expression are highlighted either orange (P-value < 0.05), green (FDR < 0.05) or blue (FDR < 0.001); whereas targets in grey show no significant difference in expression. Genes with significantly differential expression are highlighted either orange (P-value <0.05), green (FDR < 0.05) or blue (FDR <0.001); whereas genes in grey show no significant difference in expression. A subset of differentially expressed genes are labeled.

Supplementary Figure S22: Differential protein expression analysis between GBM and gcGBM. Volcano showing the differential protein expression profiles for (A) CD45- and (B) GFAP-enriched segments within the left frontal lobe. Proteins with significantly differential expression are highlighted either orange (P-value <0.05), green (FDR < 0.05) or blue (FDR < 0.001), whereas proteins in grey show no significant difference in expression. A subset of differentially expressed proteins are labeled.
FIGURE 4 from Ultra High-plex Spatial Proteogenomic Investigation of Giant Cell Glioblastoma Multiforme Immune Infiltrates Reveals Distinct Protein and RNA Expression Profiles
Authors: Bonnett Shilah A. , Rosenbloom Alyssa B. , Ong Giang T. , Conner Mark , Rininger Aric B.E. , Newhouse Daniel , New Felicia , Phan Chi Q. , Ilcisin Saskia , Sato Hiromi , Lyssand John S. , Geiss Gary , Beechem Joseph M.
Published: May 2023

Spatial proteogenomics across mouse tissue types. High-plex SPG characterization of mouse tissues with matched circular ROIs. FFPE sections were stained with GeoMx Mouse WTA (RNA control), 15-stacked GeoMx Mouse Protein Modules (137-plex; protein control), or both analytes simultaneously with the SPG workflow. A, Concordance and representative images of mouse tissue used in the assessment of the SPG versus the respective RNA (top) and protein (bottom) control. Unsupervised hierarchical clustering of top 400 expressing RNA targets with an SNR ≥ 4 (B) and detected protein targets (SNR ≥ 3; C) across all tissue types.
Supplementary Table S4 from Chemokine analysis in patients with metastatic uveal melanoma suggests a role for CCL21 signaling in combined epigenetic therapy and checkpoint immunotherapy
Authors: Sah Vasu R , Jespersen Henrik , Karlsson Joakim , Nilsson Lisa M , Bergqvist Mattias , Johansson Iva , Carneiro Ana , Helgadottir Hildur , Levin Max , Ullenhag Gustav J. , Ståhlberg Anders , Olofsson Bagge Roger , Nilsson Jonas A. , Ny Lars
Published: May 2023

Follow up adverse events (AE) of grade ≥3 (n = 29 patients)
Supplementary Table S1 from Chemokine analysis in patients with metastatic uveal melanoma suggests a role for CCL21 signaling in combined epigenetic therapy and checkpoint immunotherapy
Authors: Sah Vasu R , Jespersen Henrik , Karlsson Joakim , Nilsson Lisa M , Bergqvist Mattias , Johansson Iva , Carneiro Ana , Helgadottir Hildur , Levin Max , Ullenhag Gustav J. , Ståhlberg Anders , Olofsson Bagge Roger , Nilsson Jonas A. , Ny Lars
Published: May 2023

Representativeness Table
Figure S6 from Chemokine analysis in patients with metastatic uveal melanoma suggests a role for CCL21 signaling in combined epigenetic therapy and checkpoint immunotherapy
Authors: Sah Vasu R , Jespersen Henrik , Karlsson Joakim , Nilsson Lisa M , Bergqvist Mattias , Johansson Iva , Carneiro Ana , Helgadottir Hildur , Levin Max , Ullenhag Gustav J. , Ståhlberg Anders , Olofsson Bagge Roger , Nilsson Jonas A. , Ny Lars
Published: May 2023

Figure S6. Analysis of clinical parameters gender (a), tumor BAP1 status (b) and LDH levels
(c-d) compared to serum levels of CCL21 (a-c) or presence of TLS-like regions in tumors.
Figure S4 from Chemokine analysis in patients with metastatic uveal melanoma suggests a role for CCL21 signaling in combined epigenetic therapy and checkpoint immunotherapy
Authors: Sah Vasu R , Jespersen Henrik , Karlsson Joakim , Nilsson Lisa M , Bergqvist Mattias , Johansson Iva , Carneiro Ana , Helgadottir Hildur , Levin Max , Ullenhag Gustav J. , Ståhlberg Anders , Olofsson Bagge Roger , Nilsson Jonas A. , Ny Lars
Published: May 2023

Figure S4. Kaplan–Meier analysis showing PFS of a) CCL13 and b) IL-21. c) Flow cytometry
plots showing CCR7 and CD45RA gating among CD3 positive cells in blood samples d) Flow
cytometry based comparison between short (n=18) and long (n=6) term survivors with
CD3+CCR7+ % (T naive, T stem cell memory and T central memory) analysis using pretreatment blood samples and e) Kaplan–Meier analysis showing Overall survival with median
CD3+CCR7+ % (n=24).
Figure S1 from Chemokine analysis in patients with metastatic uveal melanoma suggests a role for CCL21 signaling in combined epigenetic therapy and checkpoint immunotherapy
Authors: Sah Vasu R , Jespersen Henrik , Karlsson Joakim , Nilsson Lisa M , Bergqvist Mattias , Johansson Iva , Carneiro Ana , Helgadottir Hildur , Levin Max , Ullenhag Gustav J. , Ståhlberg Anders , Olofsson Bagge Roger , Nilsson Jonas A. , Ny Lars
Published: May 2023

Figure S1. a) Time To Response (TTR) according to RECIST 1.1. b) Duration of Response
(DOR) according to RECIST 1.1. c) Quality of life assessments: Boxplot of EQ-5D VAS
evaluation, screening and last known assessment at database lock. d) EQ-5D VAS, change from
screening to last known assessment. e) The functional Assessment of Cancer Therapy – General
(FACT-G) score sub-scales at screening and at last known assessments
Small bowel obstruction by enterolith in an elderly woman: a case study
Authors: Teles Ricardo , Viggiano Giovanni Ferreira , Franco Geovana Louise , Abe Guilherme de Matos , Teixeira Otávio Augusto de Paula Mendes , Gomes João Victor Pereira , Lima Fernando Gonçalves , Afiune Gustavo Abrahão
Published: May 2023

Enteroliths are intraluminal calculi formed in the small bowel, commonly caused by chronic intestinal constipation associated with other comorbidities. We describe an atypical case of enterolithiasis diagnosed with computed tomography scan (CT) and which was confirmed during subsequent surgical resection. A 66-year-old female patient had a history of chronic constipation and use of laxatives and presented with abdominal pain. A CT scan showed heterogeneous annular formations with a hyperdense halo and a hypodense center within the loops of small bowel. A segment was resected for enterectomy, allowing several yellowish stony structures to be identified, corresponding to enteroliths.
A Critical Role for ERO1α in Arterial Thrombosis and Ischemic Stroke
Authors: Jha Vishwanath , Xiong Bei , Kumari Tripti , Brown Gavriel , Wang Jinzhi , Kim Kyungho , Lee Jingu , Asquith Nathan , Gallagher John , Asherman Lillian , Lambert Taylor , Bai Yanyan , Du Xiaoping , Min Jeong-Ki , Sah Rajan , Javaheri Ali , Razani Babak , Lee Jin-Moo , Italiano Joseph E. , Cho Jaehyung
Published: May 2023

Background:

Platelet adhesion and aggregation play a crucial role in arterial thrombosis and ischemic stroke. Here, we identify platelet ERO1α (endoplasmic reticulum oxidoreductase 1α) as a novel regulator of Ca
2+
signaling and a potential pharmacological target for treating thrombotic diseases.

Methods:

Intravital microscopy, animal disease models, and a wide range of cell biological studies were utilized to demonstrate the pathophysiological role of ERO1α in arteriolar and arterial thrombosis and to prove the importance of platelet ERO1α in platelet activation and aggregation. Mass spectrometry, electron microscopy, and biochemical studies were used to investigate the molecular mechanism. We used novel blocking antibodies and small-molecule inhibitors to study whether ERO1α can be targeted to attenuate thrombotic conditions.

Results:

Megakaryocyte-specific or global deletion of Ero1α in mice similarly reduced platelet thrombus formation in arteriolar and arterial thrombosis without affecting tail bleeding times and blood loss following vascular injury. We observed that platelet ERO1α localized exclusively in the dense tubular system and promoted Ca
2+
mobilization, platelet activation, and aggregation. Platelet ERO1α directly interacted with STIM1 (stromal interaction molecule 1) and SERCA2 (sarco/endoplasmic reticulum Ca
2+
-ATPase 2) and regulated their functions. Such interactions were impaired in mutant STIM1-Cys49/56Ser and mutant SERCA2-Cys875/887Ser. We found that ERO1α modified an allosteric Cys49-Cys56 disulfide bond in STIM1 and a Cys875-Cys887 disulfide bond in SERCA2, contributing to Ca
2+
store content and increasing cytosolic Ca
2+
levels during platelet activation. Inhibition of Ero1α with small-molecule inhibitors but not blocking antibodies attenuated arteriolar and arterial thrombosis and reduced infarct volume following focal brain ischemia in mice.

Conclusions:

Our results suggest that ERO1α acts as a thiol oxidase for Ca
2+
signaling molecules, STIM1 and SERCA2, and enhances cytosolic Ca
2+
levels, promoting platelet activation and aggregation. Our study provides evidence that ERO1α may be a potential target to reduce thrombotic events.
The ha-index: The average citation
h-index
Authors: Fassin Yves
Published: May 2023

The ranking and categorizations of academic articles of a data set have traditionally been based on the distribution of their total citations. This ranking formed the basis for the definition of the h-index. As an alternative methodology, the ranking of articles of a data set can be performed according to the distribution of the average citations of the articles. Applying this same principle to the h-index itself leads to an average h-index, the ha-index: the largest number of papers ha published by a researcher who has obtained at least ha citations per year on average. The new ha-index offers more consistency, increased selectivity, and fairer treatment of younger scholars compared to the classic h-index. With its normalized time aspect, the method leads to better acknowledgment of progress. The evolution of the h-indices over time shows how the ha-index reaches its full potential earlier and offers more stability over time. The average citation ha-index partly solves the problem of the temporality of the h-index. he ha-index can also be applied to academic journals. In particular, the application of the ha-index to journals leads to more stability as they reach their limit sooner. The ha-index offers a response to the inflation of h-index levels.
Negatively-Formatted Requests for Confirmation in Korean Conversation: Three Types of Verbal Negation as Interactional Resources
Authors: Kim Kyu-hyun
Published: May 2023

From a conversation-analytic perspective, this article analyzes three types of verbal negation in Korean, which shape requests for confirmation (RfCs) into distinctive interactional resources. The pre-verbal negation (an) RfC emerges in the context where the recipient is prompted to see the “fittedness” of its confirmable as referencing an “allusive” aspect of his/her situated domain, inferentially formulated by the questioner as a “negative event.” The post-verbal negation (ci anh) renders the RfC a vehicle for making mitigated assertions, seeking the recipient’s agreement in acquiescence to the questioner’s “problematizing” stance. The use of post-nominalization negation (nun-ke ani) RfC exhibits the questioner’s “conjecturing” stance, displaying norm-based deontic orientations treating the noted event at hand as deviant or counter-expectational. The target of the confirmable is formulated an “entity” assessable by the recipient as an externally-positioned co-member/evaluator. Cross-linguistic implications of the findings are discussed, in relation to English tag questions and negative interrogatives.
A new species of Notonyx A. Milne-Edwards, 1873 (Crustacea: Decapoda: Brachyura: Goneplacidae) from the Philippines
Authors: CLARK PAUL F. , NG PETER K. L.
Published: May 2023

A new species of Notonyx A. Milne-Edwards, 1873 (Crustacea: Brachyura: Goneplacidae) is described from the Sulu Archipelago, southern Philippines. It is closest to the type species, N. nitidus A. Milne-Edwards, 1873, but differs in the form of the abdomen and third maxillipeds, and most significantly, in the structures of the male first and second gonopods.
A new species of Notonyx A. Milne-Edwards 1873 (Crustacea: Decapoda: Brachyura: Goneplacidae) from Maluku, Indonesia
Authors: RAHAYU DWI LISTYO
Published: May 2023

A new species of Notonyx A. Milne-Edwards, 1873 (Crustacea: Decapoda: Brachyura: Goneplacidae) is described from Maluku, Indonesia and differs from closely related species, N. latus Ng & Clark, 2008 and N. sagittifer Ng & Clark 2010, in that the male abdomen is relatively narrower and the tip of the male second gonopod is sickled-shaped.
On some rhopalodinid sea cucumbers in the collections of the Natural History Museum, U.K. (Echinodermata: Holothuroidea: Dactylochirotida)
Authors: THANDAR AHMED S. , ARUMUGAM PREYAN
Published: May 2023

Several specimens of holothuroids in the collections of the Natural History Museum, U.K., identified as Rhopalodina lageniformis or simply Rhopalodina sp., were re-examined. Amongst these only the holotype of R. lageniformis is referable to this species. Of the four specimens misidentified as R. lageniformis, two are re-identified as R. parvalamina Cherbonnier and two described as a new species, R. cabrinovici. Another specimen, originating from Ghana, which is incorrectly identified as Rhopalodina sp., turned out to be a juvenile of Cucumaria congoana Heding and is subject of another paper describing additions to the holothuroid fauna of Ghana.
Rediscovery of Bipalium admarginatum de Beauchamp, 1933 (Platyhelminthes, Tricladida, Geoplanidae) in Malaysia, with molecular characterisation including the mitogenome
Authors: SOO OI YOON MICHELLE , GASTINEAU ROMAIN , VERDON GEORGE , WINSOR LEIGH , JUSTINE JEAN-LOU
Published: May 2023

We present here the first observation of Bipalium admarginatum de Beauchamp, 1933 since its original description 90 years ago. Three specimens were found on Perhentian Kecil Island, off Terengganu State, Malaysia and photographed in the field, and two were collected. This report thus includes the first colour photographs published for this species, from a locality close to the type-locality, Tioman Island (which is ca. 200 km south of the locality in this study, on the east coast of Peninsula Malaysia). We describe the external morphology and colour pattern of the species, which correspond well to the original description, itself based only on two preserved specimens. We performed an in-depth molecular characterisation of the species, including its complete mitochondrial genome, the 18S sequence and elongation 1-alpha (EF1-α) sequence. In addition, EF1-α sequences were also retrieved for 5 additional geoplanid species. No tRNA-Thr could be detected in the mitogenome of B. admarginatum, a lack already reported in several species of geoplanids, but we found a 13 bp sequence that contains the anticodon loop and seems to be conserved among geoplanids and might thus possibly represent a non-canonical undetected tRNA. We discuss the difficulties encountered in trying to reconstruct the cluster of nuclear ribosomal genes, a problem already mentioned for other Triclads. Three phylogenies, based respectively on all mitochondrial proteins, 18S, and EF1-α, were computed; the position of B. admarginatum within the Bipaliinae was confirmed in each tree, as sister-group to various bipaliine species according to the sequences available for each tree. In the mitochondrial proteins tree, which had high support, B. admarginatum was sister to Bipalium kewense and Diversibipalium multilineatum.
A new species of Olixon Cameron, 1887 (Hymenoptera: Rhopalosomatidae) and new records for the genus in Brazil
Authors: BULBOL MAYARA MEDEIROS , BARTHOLOMAY PEDRO RECK , OLIVEIRA MÁRCIO LUIZ DE , SOMAVILLA ALEXANDRE
Published: May 2023

Olixon Cameron, 1887 is a genus of solitary wasps belonging to Rhopalosomatidae with 28 species worldwide, all of which are distinct being brachypterous and having the inner eye margins straight. In this paper, we describe Olixon pantanensis Bulbol, Bartholomay & Somavilla sp. nov., based on a male from the Brazilian Pantanal biome and provide new distribution records for O. testaceum Cameron, 1887 in the country. We also provide the first descriptions and illustrations of the male genitalia for both species.
Structural, Crystallization Kinetics and Physical Properties of Se85Te15−xAgx Chalcogenide Glasses
Authors: Khan Shamshad A. , Khan Imtayaz H. , Akhtar M. Shaheer , Ekmekci Ismail , Kim Tae-Geum , Hashem Mohamed , Alfrisany Najm M. , Fouad Hassan , Srivastava Archana
Published: May 2023

In this study, Se85Te15−xAgx (x = 3, 6, 9 and 12) chalcogenide glasses were examined for their structure, crystallization kinetics, and physical characteristics. The kinetics of crystallization in these glasses were studied
using various methods. By using the melt quenching process, Se85Te15−xAgx bulk alloys were created. The amorphous nature of the alloys was confirmed using High Resolution X-Ray Diffraction (HRXRD). The crystallization kinetics of the Se85Te15−xAgx
glasses were studied using non-isothermal differential scanning calorimetry (DSC) measurements at heating speeds of 5, 10, 15, 20 and 25 K/min. The different characteristic temperatures, including the glass transition (Tg) and on-set crystallization (Tc)
temperatures, have been determined from a variety of DSC thermograms. Using the Kissinger and Moynihan techniques, the activation energies of the glass transition (ΔEt) were computed.
Long Non-Coding RNA TMPO-AS1 Promotes Malignant Behaviors of Hepatocellular Carcinoma Cells via YWHAG/ERK Pathway
Authors: Xie Xiaoxiao , Qin Le , Dong Fenglei , Zhu Xiaoli
Published: May 2023

Increasing attention has been paid to long noncoding RNAs (lncRNAs) as they have been implicated in the tumorigenesis of many malignancies, such as hepatocellular carcinoma (HCC). The current research explored the pro-tumorigenic biological function of the lncRNA, TMPO-AS1, in HCC,
and uncovered the underlying mechanism. The correlations between TMPO-AS1 expression and clinical outcome in HCC were analyzed based on GEO and KM plotter databases. The differential expression of TMPO-AS1 between normal liver and HCC tissues/cells was analyzed by real-time quantitative PCR.
HepG2 and Huh7 cell lines were exploited in this study. The protein expression levels were analyzed by Western blot. Cell viability was determined by CCK-8 and colony formation experiments; while cell migration and invasion capacities were observed by wound healing and Transwell, respectively.
RNA pulldown and RIP assay were adopted to determine the interaction between TMPO-AS1 and YWHAG. The TMPO-AS1 expression in HCC tissues was significantly higher than that in the normal tissues, and positively correlated with the tumor grade and poor prognosis in HCC patients. The ERK signaling
pathway could be activated by overexpressing TMPO-AS1 but repressed by silencing TMPO-AS1 in HCC cells. Mechanically, TMPO-AS1 directly interacted with YWHAG and up-regulated its protein expression by inhibiting its ubiquitination. The effects causing by TMPO-AS1 overexpression and silence
on the cell proliferation, migration, invasion, and EMT process of HCC cells could be blocked by YWHAG silence and overexpression, respectively. TMPO-AS1 may facilitate malignant behaviors of HCC cells by YWHAG/ERK pathway.
Curcumin-Loaded Nanocomposite Hydrogel Augments Functional Muscle Recovery and Shows Synergistic Effects with Post-Injury Treadmill Exercise in a Muscle Injury Model in Rats: An In Vitro and In Vivo Study
Authors: Chun Chen Xiao
Published: May 2023

Herein, the synergistic healing activity of treadmill exercise and curcumin-delivering hydrogel was evaluated in a rat model of skeletal muscle injury. In Vitro experiments were utilized to characterize the filler material and the healing potential of the treatment strategy was
investigated in a rat model of anterior tibialis muscle injury. Study showed excellent biocompatibility of the hydrogel system. In Vivo study showed that the developed hydrogel and treadmill sport regimen significantly augmented muscle function recovery as evidenced by histopathological
analysis. Gene expression studies showed that tissue expression levels of NFK-B and collagen type 1 genes were significantly downregulated in the animals treated with curcumin-loaded hydrogels with and without exercise.
Systemic Therapy in Elderly Patients With Her2/Neu-Positive Breast Cancer: A SEER Database Study
Authors: Wilbers Ashley , Quinn Karson R. , Okut Hayrettin , Helmer Stephen D. , Tenofsky Patty L.
Published: May 2023

Background

The use of systemic therapy in elderly patients with Her2/neu-positive breast cancers has been questioned given the potential for cardiac side effects with several of the agents frequently used. This study aimed to evaluate trends in use of systemic therapy in patients 70 years and older.

Methods

The 2010-2016 SEER database was used to collect data on female patients with non-metastatic Her2/neu-positive breast cancer. Data was stratified to compare systemic therapy use in patients <70 vs ≥70.

Results

A total of 62,014 patients were included in the study. Of those, 79.0% (38,760) of patients <70 years old received systemic therapy while only 45.2% (5844) of patients ≥70 received systemic therapy ( P < .001). Of patients ≥70 with ER positive tumors, 42.1% received systemic therapy and those with ER negative tumors, 52.1% received systemic therapy. The mortality rate in patients ≥70 was 8.5% in those who received systemic therapy and 12.1% in those who did not ( P < .001).

Conclusions

There remains a significant difference in rates of systemic therapy administration in the elderly population with an associated increase in mortality due to their cancer. Continuing educational efforts could be of benefit.
The Editor’s Note: The learning never ends
Authors: Preston Teresa
Published: May 2023

The practice of teaching requires ongoing learning, says Teresa Preston, Kappan’s editor-in-chief. The May 2023 issue of Kappan explores how teacher education and professional development programs can better meet the needs of current and future educators.
Centering educators of color in teacher preparation
Authors: Tamerat Jalene , Lee Clifford
Published: May 2023

Traditional teacher education and alternative teacher preparation programs struggle to recruit and retain prospective and early-career educators of color. To address this challenge, Jalene Tamerat and Clifford Lee recount their experiences as teachers and teacher educators-of color. They propose a reframing of pedagogies, curricula, and programmatic structures so they will more effectively center the cultural assets and critical perspectives among future educators of color. They share their personal reflections and offer an overview of the current educational landscape to contextualize and ground their recommendations for how teacher training programs can be more attuned to the needs of teacher candidates from minoritized and historically marginalized backgrounds.
Construction of color solid by gridded color mixing of nine primary colored fibers and spinning of full color gamut yarn
Authors: Sun Xianqiang , Xue Yuan , Xue Jingli , Jin Guang
Published: May 2023

Aiming at the demand of spinning full color gamut yarn, this paper builds a gridded color mixing mode for nine primary colored fibers containing 213 grid points and a cylindrical color solid according to the gridded cylindrical color model, and gives the equal lightness, equal hue and equal saturation chromatography matrix of the cylindrical color solid. On the basis of the three-channel computer numerical control (CNC) spinning platform, the three-element spinning mechanism of “digital color mixing spinning process – mixing ratios of colored fibers – colors of forming yarns” has been established, and the processing approach of full color gamut yarns has been provided. According to the 213 grid points of color matching surface of high, medium and low lightness of cylindrical color solid, colored yarn was prepared, and indexes of unevenness, surface hairiness and tensile strength of colored yarns were measured as well, which proved the feasibility of spinning full color gamut yarn.
Severe depressive symptoms in patients with hypertension: Are antihypertensive medications implicated?
Authors: Gammoh Omar Salem , Alqudah Abdelrahim , Abu shaikh Hanan , Al-Shudifat Abdel-Ellah
Published: May 2023

Objective

This study examined whether antihypertensive medications and other patient characteristics are associated with severe depressive symptoms in patients with hypertension.

Methods

Patients with a diagnosis of hypertension were recruited from the internal medicine outpatient clinics of a hospital in Amman, Jordan, into this cross-sectional study. Depression severity was assessed using the Patient Health Questionnaire-9 (PHQ-9); anxiety by the General Anxiety Disorder-7; sleep quality by the Insomnia Severity Index; and psychological stress by the Perceived Stress Scale. Multivariable binary logistic regression was used to examine the association between the different classes of antihypertensive medication and depressive symptoms.

Results

Of the 431 participants, 282 (65.4%) were men; 240 (55.7%) reported having type 2 diabetes; 359 (83.3%) had dyslipidemia; 142 (32.9%) were on beta-blockers; 197 (45.2%) were on ACE inhibitors or angiotensin receptor blockers; 203 (47.1%) were on metformin; and 133 (30.9%) were taking sulfonylurea. Severe depressive symptoms, indicated by scoring above the cut-off of 14 on the PHQ-9, were present in 165 (38.3%) patients. Severe depression was associated with younger age (<55 years) (OR = 3.15, 95% CI = 1.83-5.41, P < 0.001), unemployment (OR = 2.15, 95% CI = 1.15-4.00, P = 0.01), diabetes (OR = 1.81, 95% CI = 1.09-3.02, P = 0.02), severe anxiety (OR = 6.40, 95% CI = 3.64-11.28, P < 0.001), and severe insomnia (OR = 4.73, 95% CI = 2.85-7.82, P < 0.001).

Conclusion

Severe depressive symptoms were not associated with antihypertensive medications or other drugs used by hypertensive patients. Younger age, diabetes, anxiety, and insomnia were the primary correlates of depression.
SKIFF: Spherical K-means with iterative feature filtering for text document clustering
Authors: Sharma Iti , Sharma Abhay , Chaturvedi Rekha , Rajpurohit Jitendra , Kumar Manoj
Published: May 2023

Text clustering has been an overlooked field of text mining that requires more attention. Several applications require automatic text organisation which relies on an information retrieval system based on organised search results. Spherical k-means is a successful adaptation of the classic k-means algorithm for text clustering. However, conventional methods to accelerate k-means may not apply to spherical k-means due to the different nature of text document data. The proposed work introduces an iterative feature filtering technique that reduces the data size during the process of clustering which further produces more feature-relevant clusters in less time compared to classic spherical k-means. The novelty of the proposed method is that feature assessment is distinct from the objective function of clustering and derived from the cluster structure. Experimental results show that the proposed scheme achieves computation speed without sacrificing cluster quality over popular text corpora. The demonstrated results are satisfactory and outperform compared to recent works in this domain.
‘Should’ve known better’: Using Lerner’s Belief in a Just World to understand how the Fraud Justice Network observe victims of online romance and investment frauds
Authors: Nataraj-Hansen Shalini
Published: May 2023

This article explores the application of Lerner’s Belief in a Just World (BJW) to online fraud. BJW finds that people tend to blame victims when their sense of justice is threatened and if there is little or no chance of obtaining compensation or justice for the victim. Specifically, BJW suggests that when victims are perceived as acting with agency, and have little chance of compensation, they are blamed for their crime. Online fraud victims are blamed by family, friends, justice agencies, and themselves, for their victimisation. Little is known about why the Fraud Justice Network (FJN), which comprises the multitude of agencies that aim to combat online fraud, blames victims. This article begins to address this gap by presenting findings of qualitative interviews with 14 FJN representatives, using thematic analysis. Three significant themes emerge about victims: ‘agency’, ‘gullibility and compensation’, and ‘deservedness of justice’. This study found that (a) victims are seen as behaviourally responsible, (b) agency is blameworthy, and (c) victims deserve justice. The study also finds evidence of BJW thinking within the FJN. Findings can inform future organisational policies on how victims of online fraud can be more effectively managed and supported, including by financial institutions or law enforcement agencies.
The many faces of mouse histiocytic sarcoma in C57BL/6J mice
Authors: Janke Laura J. , Rehg Jerold E.
Published: May 2023

Histiocytic sarcoma is a tumor of the hematopoietic system considered to be derived from macrophages. Although rare in humans, it occurs frequently in mice. Histiocytic sarcoma can be a difficult tumor to diagnose due to its diverse cellular morphologies, growth patterns, and organ distributions. The varying morphology of histiocytic sarcomas makes it easy to confuse them with other types of neoplasia, including hepatic hemangiosarcoma, uterine schwannoma, leiomyosarcoma, uterine stromal cell tumor, intramedullary osteosarcoma, and myeloid leukemia. As such, immunohistochemistry (IHC) is often needed to differentiate histiocytic sarcomas from other common tumors in mice that they can morphologically mimic. The goal of this article is to present a broader perspective of the diverse cellular morphologies, growth patterns, organ distributions, and IHC labeling of histiocytic sarcomas encountered by the authors. This article describes these features in a set of 62 mouse histiocytic sarcomas, including the IHC characterization of the tumors using a panel of markers for the macrophage antigens F4/80, IBA1, MAC2, CD163, CD68, and lysozyme, and describes differentiating features of histiocytic sarcomas from other morphologically similar tumors. The genetic changes underlying the pathogenesis of histiocytic sarcoma in humans are beginning to be elucidated, but this is difficult due to its rarity. The higher prevalence of this tumor in mice provides opportunities to investigate mechanisms of its development and to test potential treatments.
Type and Dosage of Performance Feedback Following COMPASS Consultation on Teacher and Student Outcomes
Authors: Ogle Lindsey N. , Ruble Lisa A. , Toland Michael D. , McGrew John H.
Published: May 2023

Little is known about differences in dosage or feedback that make teacher coaching effective in relation to teacher and student outcomes. This study builds upon previous research on the Collaborative Model for Promoting Competence and Success (COMPASS) to understand the impact of different types (face-to-face coaching vs. emailed feedback) and dosages (one vs. two or four) of performance feedback compared to receiving no feedback following an initial consultation during which intervention plans were developed. Findings suggest that teacher adherence and student goal-attainment outcomes depend on dosage, not type of coaching. Specifically, having two or four opportunities for performance feedback was significantly better than having none or only one opportunity. Ratings of teacher adherence and student goal attainment delivered via emailed feedback or face-to-face coaching were similar, which may have important implications for cost and efficiency. While preliminary, the results are promising and warrant further research. Implications are also discussed.
Patients’ Perceptions of Physical Therapists Addressing Opioid Misuse
Authors: Magel John , Hartman Paul , Fritz Julie M. , Koch Nicholas N. , Dostal Hannah , Vollmer Nicholas , Ferguson Natalie L. , Tapken Jennifer , Cohee Kim , Cochran Gerald , Gordon Adam J.
Published: May 2023

Introduction:

In the US, rising numbers of patients who misuse illicit or prescribed opioids provides opportunities for physical therapists (PTs) to be engaged in their care. Prior to this engagement, it is necessary to understand the perceptions of patients who access physical therapy services about their PTs playing such a role. This project examined patients’ perceptions of PTs addressing opioid misuse.

Methods:

We surveyed patients, newly encountering outpatient physical therapy services in a large University-based healthcare setting, via anonymous, web-based survey. Within the survey, questions were rated on a Likert scale (1 = completely disagree to 7 = completely agree) and we evaluated responses of patients who were prescribed opioids versus those who were not.

Results:

Among 839 respondents, the highest mean score was 6.2 (SD = 1.5) for “It is OK for physical therapists to refer their patients with prescription opioid misuse to a specialist to address the opioid misuse.” The lowest mean score was 5.6 (SD = 1.9) for “It is OK for physical therapists to ask their patient why they are misusing prescription opioids.” Compared to those with no prescription opioid exposure while attending physical therapy, patients with prescription opioid exposure had lower agreement that it was OK for the physical therapist to refer their patients with opioid misuse to a specialist (β = −.33, 95% CI = −0.63 to −0.03).

Conclusions:

Patients attending outpatient physical therapy seem to support PTs addressing opioid misuse and there are differences in support based on whether the patients had exposure to opioids.
Cognitive Behavioral Therapy for Depression Among Chinese Diabetes Patients: A Systematic Review and Meta-Analysis
Authors: Yu Zhan , An Qiuling , Hawkins Jaclynn , Zhang Anao
Published: May 2023

Objective: The purpose of this study was to evaluate the effectiveness of cognitive behavioral therapy (CBT) for Chinese diabetic patients suffering from depression. Method: Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines, we researched seven electronic databases and two professional websites. Publication bias was assessed using funnel plot. Meta-analysis was conducted using meta-regression with robust variance estimation. Results: Final analysis included a total of 23 controlled trials containing 201 effect size estimates (including 5025 participants). Subgroup analyses indicated significant treatment effects for (1) depression outcomes, anxiety outcomes, psychological stress/distress outcomes, physiological outcomes, and general wellness outcomes, (2) studies with/without manuals, (3) studies using individual-based/group-based CBT, (4) studies in person/assisted with technology, and (5) studies providers with/without training. The treatment outcomes and intervention composition (CBT only versus CBT plus other approaches) were significant moderators. Conclusions: Findings of the study suggested CBT is a promising treatment option for depression among Chinese diabetes patients.
Dual Schooling for Low-Resource Families: An Exploratory Study From a Positive Deviance Perspective
Authors: Chong Wan Har , Tanuwira Juliet , Nalawala Nandita , Wong Shi Ning
Published: May 2023

Parent education programs are important avenues through which parents of children with developmental delays can learn new skills to help them with child-rearing challenges. However, those in economic hardship often face problems with applying these skills at home where the child’s learning and/or behavioral problems commonly occur. In this exploratory qualitative study, we adopted a positive deviance (PD) lens to explore strategies six economically disadvantaged parents used to support their children who were attending special and mainstream preschools concurrently. The PD perspective was first used in public health research with low-resource communities where a few individuals have been observed to adopt uncommon practices and behaviors that enable them to find better ways to prevent social or health problems than their neighbors facing similar constraints and risks. We identified adaptive parental behaviors, thoughts and feelings that helped them navigate daily hassles faced with “dual schooling” their children in Singapore. The study highlights important implications in the development and refinement of future parent training programs.
Intra-Abdominal and Retroperitoneal Benign Lipomatous Tumors—An Extremely Rare Mimic of Liposarcoma and its Diagnostic Challenge
Authors: Malik Faizan , Allbee Andrew W. , Zhang Paul J.
Published: May 2023

Background. Lipomas are common superficial soft tissue tumors of mature adipocytes. In contrast, well-differentiated/dedifferentiated liposarcoma typically presents in the retroperitoneum as large masses. We provide clinicopathologic and follow-up details of 9 retroperitoneal/intra-abdominal benign lipomatous tumors (BLT) and discuss the utility of ancillary fluorescence in situ hybridization (FISH) in distinguishing from their malignant counterparts. Design. Clinicopathologic details and histology of 9 intra-abdominal and retroperitoneal lipomas were studied along with ancillary CD10 immunohistochemistry (IHC) and FISH for MDM2 and CDK4 amplification. Results. There were 6 females and 3 males. Median age at diagnosis was 52 years (range 36-81 years). Seven were identified incidentally and 2 presented with primary complaints. On imaging, 7 were considered suspicious for liposarcoma. Grossly, the tumors ranged from 3.4 to 41.2 cm (median 16.5 cm). Histologically, all cases showed well-differentiated BLT, further classified as lipoma (n = 7; 1 with metaplastic ossification, 2 with prominent vessels, and 4 ordinary lipomas) and lipoma-like hibernoma (n = 2)—the latter 2 showed intramuscular lesions with interspersed brown fat. CD10 IHC showed strong staining in the 2 hibernomas, whereas the staining was weak in the remaining. MDM2 and CDK4 amplification were negative by FISH in all. Follow-up (median 18 months) did not show recurrence on clinical or imaging evaluation. Conclusion. Retroperitoneal/intra-abdominal BLT are extremely rare and are indistinguishable clinically and radiographically from liposarcoma. This necessitates molecular confirmation even when the histology is convincingly benign, for a confident diagnosis. Our cohort shows that conservative excision without removal of abutted organs is sufficient in most cases.
Frequency of Hepatic Metastatic Disease in Patients with Stage IV Breast Cancer Is Similar for Steatotic and Non-Steatotic Livers
Authors: Haq Adeel , Fraum Tyler J , Tao Yu , Dehdashti Farrokh , LeBlanc Maverick , Hoegger Mark J , Luo Jingqin , Weilbaecher Katherine , Peterson Lindsay L
Published: May 2023

Background:

Breast cancer is the most common non-cutaneous malignancy and the second leading cause of cancer mortality in the United States. Breast cancer is a heterogeneous disease; diagnosis at an early stage renders it potentially curable, whereas advanced metastatic disease carries a worse prognosis.

Objectives:

To investigate whether hepatic steatosis (HS) is associated with liver metastases in patients with newly diagnosed stage IV female breast cancer patients (either de novo metastatic breast cancer or recurrent metastatic breast cancer) using non-contrast computed tomography (CT) as a marker of HS.

Design:

Retrospective analysis.

Methods:

We retrospectively identified 168 patients with stage IV breast cancer with suitable imaging from a prospectively maintained oncologic database. Three radiologists manually defined hepatic regions of interest on non-contrast CT images, and attenuation data were extracted. HS was defined as a mean attenuation <48 Hounsfield units. The frequency of hepatic metastatic disease was calculated for patient with and without HS. Relationships between HS and various patient (age, body mass index, race) and tumor (hormone receptor status, HER2 status, tumor grade) characteristics were also analyzed.

Results:

There were 4 patients with liver metastasis in the HS group (41 patients) versus 20 patients with liver metastases in the non-HS group (127 patients). The difference in frequencies of liver metastases among patients with (9.8%) versus without (15.7%) hepatic steatosis (odds ratio = 1.72 [0.53-7.39]) was not statistically significant ( P = .45). Body mass index was significantly higher ( P = .01) among patients with hepatic steatosis (32.2 ± 7.3 vs 28.8 ± 7.1 kg/m2). Otherwise, there were no significant differences between patients with versus without HS with respect to regarding age, race, hormone receptor status, HER2 status, or tumor grade.

Conclusion:

The frequency of hepatic metastatic disease in patients with stage IV breast cancer is similar for steatotic and non-steatotic livers.
Effectiveness and Safety of Multicomponent Physical Training in Patients With Chronic Obstructive Pulmonary Disease: Protocol for a Randomized Clinical Trial
Authors: Mazzarin Camila Monteiro , Silveira Bruna Roberta , Lamezon Ana Cristina , Cavon Luna Bruna , Valderramas Silvia
Published: May 2023

Background:

Currently, most Pulmonary Rehabilitation (PR) programs work with conventional physical training, using resources that are not available in public health in Brazil. Multicomponent physical training is a strategy that uses few resources and can reach a larger portion of the population.

Objective:

To investigate the effectiveness and safety of multicomponent physical training on physical-functional performance in patients with COPD.

Design:

Protocol for a randomized clinical trial with 2 groups in parallel (1:1).

Setting:

University-based, outpatient, physiotherapy clinic.

Participants:

64 patients aged ⩾50 years, clinical-functional diagnosis of COPD, GOLD II and III criteria will participate in the study.

Intervention:

Participants will be randomly allocated into 2 groups: Multicomponent Physical Training—MPT (n = 32): aerobic, strength, balance and flexibility exercises performed in a circuit training format, or Conventional Physical Training—CPT (n = 32): aerobic and strength training. The interventions will be carried out twice a week, for 8 weeks and supervised by the same physiotherapist.

Measurements:

The 3 primary outcomes are the 6-Minute Walk Test (6MWT), the 6-Minute Step Test (6MST) and VO2 consumption as measured during the 6MWT. Secondary outcomes will be exercise capacity, level of physical activity in daily life, peripheral muscle strength, functional status, dyspnea, fatigue and quality of life. Safety will be assessed by recording adverse effects. These outcomes will be evaluated before and after the intervention and the evaluator will be blind.

Limitations:

It will not be possible to perform the blinding of the physiotherapist who will supervise the interventions.

Conclusions:

This study is expected to demonstrate that MPT using simple resources is an effective and safe intervention for the improvement of the aforementioned outcomes and, in addition, to broaden the horizon of research in relation to new methods of physical rehabilitation for patients with COPD.