bluuebunny/crossref_metadata_2025 huggingface dataset:
A20 Attenuates the Fibrotic Response in the Trabecular Meshwork
Authors: Mzyk Philip , Zalog Emma G. , McDowell Colleen M.
Published: February 2022

Although the extracellular matrix (ECM) in trabecular meshwork (TM) cells is known to be important in intraocular pressure (IOP) regulation, the molecular mechanisms involved in generating a glaucomatous environment in the TM are not completely understood. Recently we identified a molecular pathway, transforming growth factor beta 2 (TGFβ2)–toll-like receptor 4 (TLR4) signaling crosstalk, as an important regulator of glaucomatous damage in the TM, which contributes to fibrosis. Here we evaluate a novel molecular target, A20, also known as tumor necrosis factor alpha-induced protein 3 (TNFAIP3), which may help to block pathological TGFβ2–TLR4 signaling. Primary human TM cells were analyzed for A20 message and for A20 and fibronectin protein expression after treatment with TGFβ2. A20 message increased when the TLR4 pathway was inhibited in TM cells. In addition, TGFβ2, a known inducer of fibrosis, increased fibronectin expression, while at the same time decreasing the expression of A20. We then overexpressed A20 in TM cells in order to test the effect on treatment with TGFβ2, lipopolysaccharide (LPS), or cellular fibronectin extra domain A (cFN-EDA). Importantly, overexpression of A20 rescued the fibrotic response when TM cells were treated with TGFβ2, LPS, or cFN-EDA. In situ hybridization was used to probe for A20 RNA expression in age-matched control (C57BL/6J) mice and mice that constitutively express the EDA isoform of fibronectin (B6.EDA+/+). In this novel mouse model of glaucoma, A20 RNA was increased versus age-matched control mice in a cyclic manner at 6 weeks and 1 year of age, but not at 8 months. Overall, these data suggest that A20 may work through a negative feedback mechanism attenuating the ability of TGFβ2–TLR4 signaling to induce fibrosis.
Revised Manuscript with Corrections: Polyurethane-Based Conductive Composites: From Synthesis to Applications
Authors: Choi Soon-Mo , Shin Eun-Joo , Zo Sun-Mi , Rao Kummara-Madhusudana , Seok Yong-Joo , Won So-Yeon , Han Sung-Soo
Published: February 2022

The purpose of this review article is to outline the extended applications of polyurethane (PU)-based nanocomposites incorporated with conductive polymeric particles as well as to condense an outline on the chemistry and fabrication of polyurethanes (PUs). Additionally, we discuss related research trends of PU-based conducting materials for EMI shielding, sensors, coating, films, and foams, in particular those from the past 10 years. PU is generally an electrical insulator and behaves as a dielectric material. The electrical conductivity of PU is imparted by the addition of metal nanoparticles, and increases with the enhancing aspect ratio and ordering in structure, as happens in the case of conducting polymer fibrils or reduced graphene oxide (rGO). Nanocomposites with good electrical conductivity exhibit noticeable changes based on the remarkable electric properties of nanomaterials such as graphene, RGO, and multi-walled carbon nanotubes (MWCNTs). Recently, conducting polymers, including PANI, PPY, PTh, and their derivatives, have been popularly engaged as incorporated fillers into PU substrates. This review also discusses additional challenges and future-oriented perspectives combined with here-and-now practicableness.
Nanohydrogels: Advanced Polymeric Nanomaterials in the Era of Nanotechnology for Robust Functionalization and Cumulative Applications
Authors: Quazi Mohzibudin Z. , Park Nokyoung
Published: February 2022

In the era of nanotechnology, the synthesis of nanomaterials for advanced applications has grown enormously. Effective therapeutics and functionalization of effective drugs using nano-vehicles are considered highly productive and selectively necessary. Polymeric nanomaterials have shown their impact and influential role in this process. Polymeric nanomaterials in molecular science are well facilitated due to their low cytotoxic behavior, robust functionalization, and practical approach towards in vitro and in vivo therapeutics. This review highlights a brief discussion on recent techniques used in nanohydrogel designs, biomedical applications, and the applied role of nanohydrogels in the construction of advanced therapeutics. We reviewed recent studies on nanohydrogels for their wide applications in building strategies for advantageously controlled biological applications. The classification of polymers is based on their sources of origin. Nanohydrogel studies are based on their polymeric types and their endorsed utilization for reported applications. Nanotechnology has developed significantly in the past decades. The novel and active role of nano biomaterials with amplified aspects are consistently being studied to minimize the deleterious practices and side effects. Here, we put forth challenges and discuss the outlook regarding the role of nanohydrogels, with future perspectives on delivering constructive strategies and overcoming the critical objectives in nanotherapeutic systems.
Aging and Clonal Behavior of Hematopoietic Stem Cells
Authors: Yamashita Masayuki , Iwama Atsushi
Published: February 2022

Hematopoietic stem cells (HSCs) are the only cell population that possesses both a self-renewing capacity and multipotency, and can give rise to all lineages of blood cells throughout an organism’s life. However, the self-renewal capacity of HSCs is not infinite, and cumulative evidence suggests that HSCs alter their function and become less active during organismal aging, leading ultimately to the disruption of hematopoietic homeostasis, such as anemia, perturbed immunity and increased propensity to hematological malignancies. Thus, understanding how HSCs alter their function during aging is a matter of critical importance to prevent or overcome these age-related changes in the blood system. Recent advances in clonal analysis have revealed the functional heterogeneity of murine HSC pools that is established upon development and skewed toward the clonal expansion of functionally poised HSCs during aging. In humans, next-generation sequencing has revealed age-related clonal hematopoiesis that originates from HSC subsets with acquired somatic mutations, and has highlighted it as a significant risk factor for hematological malignancies and cardiovascular diseases. In this review, we summarize the current fate-mapping strategies that are used to track and visualize HSC clonal behavior during development or after stress. We then review the age-related changes in HSCs that can be inherited by daughter cells and act as a cellular memory to form functionally distinct clones. Altogether, we link aging of the hematopoietic system to HSC clonal evolution and discuss how HSC clones with myeloid skewing and low regenerative potential can be expanded during aging.
Towards an Engaging and Gamified Online Learning Environment—A Real CaseStudy
Authors: Portela Filipe
Published: February 2022

Currently, remote work is common, and this trend has come to several areas and processes, such as education and teaching. Regarding higher education, universities have several challenges to overcome, the most challenging being transforming teaching to be more digital and engaging. Therefore, TechTeach has arisen as a new teaching paradigm that creates a unique learning environment and satisfies students’ and professors’ expectations. After the success of the b-learning approach, professors created new experiences utilizing an entirely online learning environment following this paradigm. This article shows the work performed through a real case study, explains the strategy used to implement this paradigm, provides students’ opinions, and analyses the results achieved. The results demonstrated that, while the effort was tremendous, the result was beneficial to all. After 208 online hours of classes, 11,173 downloads, 15,224 messages, 200,000 sessions, 3 rescues requests, and 28t cards, 98.15% of the active participants gave it their approval, 96.53% considered this subject equal to or better than the others, and 85% of accepted the gamification system. These results show that a class can be an engaging environment where students can learn and enjoy it regardless of whether it is physical or not.
Architecture of a Hybrid Video/Optical See-through Head-Mounted Display-Based Augmented Reality Surgical Navigation Platform
Authors: Carbone Marina , Cutolo Fabrizio , Condino Sara , Cercenelli Laura , D’Amato Renzo , Badiali Giovanni , Ferrari Vincenzo
Published: February 2022

In the context of image-guided surgery, augmented reality (AR) represents a ground-breaking enticing improvement, mostly when paired with wearability in the case of open surgery. Commercially available AR head-mounted displays (HMDs), designed for general purposes, are increasingly used outside their indications to develop surgical guidance applications with the ambition to demonstrate the potential of AR in surgery. The applications proposed in the literature underline the hunger for AR-guidance in the surgical room together with the limitations that hinder commercial HMDs from being the answer to such a need. The medical domain demands specifically developed devices that address, together with ergonomics, the achievement of surgical accuracy objectives and compliance with medical device regulations. In the framework of an EU Horizon2020 project, a hybrid video and optical see-through augmented reality headset paired with a software architecture, both specifically designed to be seamlessly integrated into the surgical workflow, has been developed. In this paper, the overall architecture of the system is described. The developed AR HMD surgical navigation platform was positively tested on seven patients to aid the surgeon while performing Le Fort 1 osteotomy in cranio-maxillofacial surgery, demonstrating the value of the hybrid approach and the safety and usability of the navigation platform.
All-in-One Mosquito Containers: From the Laboratory to the Release Sites
Authors: Tur Carlos , Plá Ignacio , Argilés-Herrero Rafael , Salvador-Herranz Gustavo , Almenar David
Published: February 2022

Integrated vector control programs that use a Sterile Insect Technique approach require the production and release of large numbers of high quality, sterile male insects. In pilot projects conducted worldwide, sterile males are usually kept in containers at low densities until their manual release on the ground. Although the quality of the released insects is high, these containers are only suitable for small-scale projects, given the fact that the manual labor required for release is significant and therefore untenable in large-scale projects. This study will compare and contrast the quality of the males reared in the proposed “all-in-one” containers which considerably reduce both the handling of the insects and the manual labor required for release. As a result, project costs are lower. The design of these “all-in-one” containers incorporates two important features: ventilation and the density of the vertical resting surface. Having evaluated both features, it can be concluded that ventilation does not directly affect the quality of the insects, at least in the range of dimensions tested. However, the quality of the male insects is reduced in relation to an increase in the number of mosquitoes, with 500 being the optimum quantity of mosquitoes per “all-in-one” container.
Quality Control Methods for Aedes albopictus Sterile Male Transportation
Authors: Mastronikolos Georgios D. , Kapranas Apostolos , Balatsos George K. , Ioannou Charalampos , Papachristos Dimitrios P. , Milonas Panagiotis G. , Puggioli Arianna , Pajović Igor , Petrić Dušan , Bellini Romeo , Michaelakis Antonios , Papadopoulos Nikos T.
Published: February 2022

Genetic based mosquito control methods have been gaining ground in recent years for their potential to achieve effective suppression or replacement of vector populations without hampering environments or causing any public health risk. These methods require the mass rearing of the target species in large facilities sized to produce millions of sterile males, as already well established for a number of insects of agricultural importance. Assessing the performance of released males in Sterile Insect Technique (SIT) control programs is of the utmost importance for the success of the operation. Besides the negative effects of mass rearing and sterilization, the handling of sterilized insects and shipment to distant areas may also negatively impact the quality of sterilized males. The aim of the current study was to design and executive quality control (QC) tests for sterilized Aedes albopictus (Asian tiger mosquito) males delivered by air shipment from the mass production facility located in Italy to Greece and Montenegro field release sites. Mass reared mosquito strains were based on biological materials received from Italy, Greece and Montenegro. Tests conducted at the mass rearing facility before transportation revealed a rather high residual female contamination following mechanical sex separation (approximately 1.5% females, regardless of the mosquito strain). Irradiated males of all three mosquito strains induced high levels of sterility to females. Shipment lasting approximately 24 h resulted in approximately 15% mortality, while when shipment lasted nearly two days this increased to almost 40%. The flight ability of sterilized males following one day transportation time was satisfactory (over 60%). The response of sterile males to food and water starvation was comparable and slightly lower than that of wild non-transported males. Longevity of sterile males was shorter than that of wild counterparts and it seems it was not affected by mating to wild females. Both mating propensity and mating competitiveness for wild virgin females was higher for the wild, control males compared to the sterile, transported ones. Overall, the performance of sterile male Ae. albopictus delivered from the mass rearing facility of Italy to Greece in approximately 24 h was satisfactory. Transportation lasting two days or longer incurred detrimental effects on males, which called into question the outcome of the SIT release programs. In conclusion, our results demonstrate the need of quality control procedures, especially when sterile male production facilities are not near to the releasing point. Transportation could be a serious drawback for the implementation of Sterile Insect Releases and, consequently, it is important to establish an efficient and fast transportation of sterilized males in advance.
Comparison of Transcriptome Responses between Sogatella furcifera Females That Acquired Southern Rice Black-Streaked Dwarf Virus and Not
Authors: Liu Dandan , Li Zhengxi , Hou Maolin
Published: February 2022

The southern rice black-streaked dwarf virus (SRBSDV) is transmitted horizontally by Sogatella furcifera in a persistent, propagative manner. Exposure of S. furcifera females to SRBSDV-infected rice plants may trigger transcriptomic changes in the insects, the transcriptomes of females that acquired SRBSDV and those that failed to, as well as females fed on healthy rice plants as control, were sequenced and compared. Nine transcriptomic libraries were constructed, from which a total of 53,084 genes were assembled. Among the genes, 1043 and 2932 were differentially expressed genes (DEGs) in S. furcifera females that acquired SRBSDV and that failed to, in comparison with the control, respectively. Functional enrichment analysis showed that DEGs identified in S. furcifera females exposed to SRBSDV are primarily involved in diverse signaling pathways related to primary metabolism and innate immunity. The DEGs in the S. furcifera females that failed to acquire the virus significantly outnumbered that in the insects that acquired the virus, and the virus exposure activated the humoral and cellular immune responses of the vectors, especially the apoptosis. The key gene in apoptosis encoding caspase 1 was upregulated by SRBSDV exposure, especially in S. furcifera females that failed to acquire the virus. Analysis of caspase 1 activity validated that SRBSDV exposure induced caspase 1 accumulation. Surprisingly, the expression of six female-specific genes was also upregulated by SRBSDV exposure, which was confirmed by RT-qPCR analysis. This study provides evidence to explain the differential virus acquisition at the transcriptome level.
Scanning Electron Microscopic Analysis of Antennal Sensilla and Tissue-Expression Profiles of Chemosensory Protein Genes in Ophraella communa (Coleoptera: Chrysomelidae)
Authors: Ma Chao , Yue Yang , Zhang Yan , Tian Zhen-Ya , Chen Hong-Song , Guo Jian-Ying , Zhou Zhong-Shi
Published: February 2022

Ophraella communa is an efficient biocontrol agent used against the invasive weed Ambrosia artemisiifolia. It is an herbivorous insect that feeds on specific plants; the olfactory functions of this insects plays an important role in their search for host plants. There are no reports on O. communa sensilla types, morphology, or chemosensory protein (CSP) genes. In this study, we observed the external structure and distribution of antennal sensilla in adult O. communa antennae by scanning electron microscopy; moreover, we cloned 11 CSPs (CSP1–CSP11) and elucidated their tissue-expression profiles using quantitative real-time polymerase chain reaction. Six types of sensilla were identified: sensilla trichodea (including two subtypes), sensilla chaetica, sensilla basiconica (including two subtypes), sensilla styloconica, sensilla coeloconica, and Böhm bristles. Both male and female antennae had all six types of sensilla, and no sexual dimorphism was noted in sensillar types or distribution. We also found that the expression levels of CSP2, CSP3, CSP4, CSP6, and CSP7 in male and female antennae were higher than those in other tissues, which suggests that these five CSPs may be related to olfactory function in O. communa. Ultimately, our results lay the foundation for interpreting the olfactory functions of adult O. communa.
Serum Levels of MMP-8 and MMP-9 as Markers in Chronic Subdural Hematoma
Authors: Su Gao-Jian , Gao Jie , Wu Chu-Wei , Zou Jun-Feng , Zhang Di , Zhu Dong-Liang , Liu Jun , Zhang Jie-Hua , Huang Xian-Jian
Published: February 2022

Chronic subdural hematoma (CSDH) is a common neurological disease that involves the collection of blood products in the subdural space. The progression of CSDH is an angiogenic and inflammatory process, but the multifactorial mechanisms underlying CSDH are still not fully understood. We aimed to identify one or more factors that may play an important role in the development of CSDH. We enrolled 83 patients with CSDH, including 17 postoperative patients, and analyzed 20 markers in the hematoma fluid and peripheral blood of each patient. Overall differential gene expression was examined to identify the representative markers. The concentration of MMP-8 was significantly lower in the postoperative group than in the preoperative group. The concentration of MMP-9 was significantly higher in the postoperative group than in the preoperative group. These findings indicate that MMP-8 and MMP-9 may play important roles in the pathophysiology of CSDH. Understanding the pathways associated with CSDH may provide insights for improving disease outcomes.
Multiple Aspects of Inappropriate Action of Renin–Angiotensin, Vasopressin, and Oxytocin Systems in Neuropsychiatric and Neurodegenerative Diseases
Authors: Szczepanska-Sadowska Ewa , Wsol Agnieszka , Cudnoch-Jedrzejewska Agnieszka , Czarzasta Katarzyna , Żera Tymoteusz
Published: February 2022

The cardiovascular system and the central nervous system (CNS) closely cooperate in the regulation of primary vital functions. The autonomic nervous system and several compounds known as cardiovascular factors, especially those targeting the renin–angiotensin system (RAS), the vasopressin system (VPS), and the oxytocin system (OTS), are also efficient modulators of several other processes in the CNS. The components of the RAS, VPS, and OTS, regulating pain, emotions, learning, memory, and other cognitive processes, are present in the neurons, glial cells, and blood vessels of the CNS. Increasing evidence shows that the combined function of the RAS, VPS, and OTS is altered in neuropsychiatric/neurodegenerative diseases, and in particular in patients with depression, Alzheimer’s disease, Parkinson’s disease, autism, and schizophrenia. The altered function of the RAS may also contribute to CNS disorders in COVID-19. In this review, we present evidence that there are multiple causes for altered combined function of the RAS, VPS, and OTS in psychiatric and neurodegenerative disorders, such as genetic predispositions and the engagement of the RAS, VAS, and OTS in the processes underlying emotions, memory, and cognition. The neuroactive pharmaceuticals interfering with the synthesis or the action of angiotensins, vasopressin, and oxytocin can improve or worsen the effectiveness of treatment for neuropsychiatric/neurodegenerative diseases. Better knowledge of the multiple actions of the RAS, VPS, and OTS may facilitate programming the most efficient treatment for patients suffering from the comorbidity of neuropsychiatric/neurodegenerative and cardiovascular diseases.
Waste Glass-Derived Tobermorite Carriers for Ag+ and Zn2+ Ions
Authors: Rahman Habib , Li Qiu , Coleman Nichola J.
Published: February 2022

In this study, the layer-lattice calcium silicate hydrate mineral, tobermorite, was synthesized from waste green or amber container glass and separately ion-exchanged with Ag+ or Zn2+ ions under batch conditions. Hydrothermal treatment of stoichiometrically adjusted mixtures of waste glass and calcium oxide in 4 M NaOH(aq) at 125 °C yielded tobermorite products of ~75% crystallinity with mean silicate chain lengths of 17 units after one week. Maximum uptake of Zn2+ ions, ~0.55 mmol g−1, occurred after 72 h, and maximum uptake of Ag+ ions, ~0.59 mmol g−1, was established within 6 h. No significant differences in structure or ion-exchange behavior were observed between the tobermorites derived from either green or amber glass. Composite membranes of the biopolymer, chitosan, incorporating the original or ion-exchanged tobermorite phases were prepared by solvent casting, and their antimicrobial activities against S. aureus and E. coli were evaluated using the Kirby–Bauer assay. S. aureus and E. coli formed biofilms on pure chitosan and chitosan surfaces blended with the original tobermorites, whereas the composites containing Zn2+-substituted tobermorites defended against bacterial colonization. Distinct, clear zones were observed around the composites containing Ag+-substituted tobermorites which arose from the migration of the labile Ag+ ions from the lattices. This research has indicated that waste glass-derived tobermorites are functional carriers for antimicrobial ions with potential applications as fillers in polymeric composites to defend against the proliferation and transmission of pathogenic bacteria.
Hybrid Hyaluronic Acid versus High Molecular Weight Hyaluronic Acid for the Treatment of Hip Osteoarthritis in Overweight/Obese Patients
Authors: Scaturro Dalila , Vitagliani Fabio , Terrana Pietro , Tomasello Sofia , Falco Vincenzo , Cuntrera Daniele , Spoto Italia , Midiri Massimo , Letizia Mauro Giulia
Published: February 2022

Background: Obesity is the main risk factor for hip osteoarthritis, negatively affecting the outcome of the disease. We evaluated the effectiveness of viscosupplementation with hybrid hyaluronic acid compared to that with high molecular weight hyaluronic acid in overweight/obese patients with hip osteoarthritis (OA). Methods: 80 patients were divided into two groups: a treatment group received two ultrasound-guided intra-articular hip injections of hybrid HA 15 days apart; a control group received a single ultrasound-guided infiltration with medium-high molecular weight hyaluronic acid (1500–2000 kDa). We assessed the pain, functional and cardiovascular capacity of the patients at baseline, after 3 months, and after 6 months of the infiltrative sessions. Results: The treatment group showed greater improvements in the scores on the NRS scale (5.4 ± 0.8 vs. 6.3 ± 0.8; p < 0.05) and in the Lequesne index (11.4 ± 2.6 vs. 13.6 ± 2.7; p < 0.05) and in the distance traveled at 6MWT (238.1 ± 53.9 m vs. 210.7 ± 46.2 m; p = 0.02) both at 3 months (T1) and at 6 months (T2). Conclusions: This study underlines the importance of exploiting the anti-inflammatory, analgesic, and chondrogenic properties of hybrid HA for the treatment of hip OA in overweight/obese patients.
Multi-Omics Analysis Reveals Myelin, Presynaptic and Nicotinate Alterations in the Hippocampus of G72/G30 Transgenic Mice
Authors: Filiou Michaela D. , Teplytska Larysa , Nussbaumer Markus , Otte David-M. , Zimmer Andreas , Turck Christoph W.
Published: February 2022

The primate-specific G72/G30 gene locus has been associated with major psychiatric disorders, such as schizophrenia and bipolar disorder. We have previously generated transgenic mice which carry the G72/G30 locus and express the longest G72 splice variant (LG72) protein encoded by this locus with schizophrenia-related symptoms. Here, we used a multi-omics approach, including quantitative proteomics and metabolomics to investigate molecular alterations in the hippocampus of G72/G30 transgenic (G72Tg) mice. Our proteomics analysis revealed decreased expression of myelin-related proteins and NAD-dependent protein deacetylase sirtuin-2 (Sirt2) as well as increased expression of the scaffolding presynaptic proteins bassoon (Bsn) and piccolo (Pclo) and the cytoskeletal protein plectin (Plec1) in G72Tg compared to wild-type (WT) mice. Metabolomics analysis indicated decreased levels of nicotinate in G72Tg compared to WT hippocampi. Decreased hippocampal protein expression for selected proteins, namely myelin oligodentrocyte glycoprotein (Mog), Cldn11 and myelin proteolipid protein (Plp), was confirmed with Western blot in a larger population of G72Tg and WT mice. The identified molecular pathway alterations shed light on the hippocampal function of LG72 protein in the context of neuropsychiatric phenotypes.
Optimizing Treatment of Parkinson’s Disease
Authors: Nyholm Dag , Bergquist Filip
Published: February 2022

The holy grail of therapy in Parkinson’s disease (PD) is treatment that would halt the disease process or restore the degenerated neuronal circuits [...]
Quantifying the Spatial-Temporal Variation of Population Urbanization and Affordable Housing Land in China
Authors: He Chunyan , Li Ding , Yu Junlin
Published: February 2022

The governments of most countries are striving to coordinate residents’ housing demands with the supply of land to achieve high-quality urbanization and improve residents’ welfare. Based on the panel data of all Chinese provinces from 2010 to 2017, this study obtains 248 observations. It uses the coupling coordination degree, the Mann–Kendall trend test, and spatial autocorrelation methods to analyze the temporal and spatial variation of the coordination degree of population urbanization and affordable housing land supply. The study aims to clarify the temporal trend further and identify the spatial mismatch of affordable housing land resource allocation and apply a panel fixed model to determine its driving factors. The regional differentiation of the coordinated development level of population urbanization and affordable housing land in China’s provinces is apparent. The overall spatial distribution characteristics of coordination are opposite to the development of its economic level. The Mann–Kendall trend test indicated that the coupling coordination degree had a significant upward trend nationwide. Moreover, the coupling coordination degree shows a strong positive global spatial correlation. The local spatial agglomeration characteristics of the coordination are significant and primarily manifested in high–high and low–low agglomeration. A negative correlation exists between the degree of coupling coordination and the dependence of local governments on land finance and housing prices.
Baseline Gene Expression Levels in Falkland-Malvinas Island Penguins: Towards a New Monitoring Paradigm
Authors: Bowen Lizabeth , Waters Shannon , Stott Jeffrey L. , Duncan Ann , Meyerson Randi , Woodhouse Sarah
Published: February 2022

Health diagnostics of wildlife have historically relied on the evaluation of select serum biomarkers and the identification of a contaminant or pathogen burden within specific tissues as an indicator of a level of insult. However, these approaches fail to measure the physiological reaction of the individual to stressors, thus limiting the scope of interpretation. Gene-based health diagnostics provide an opportunity for an alternate, whole-system, or holistic assessment of health, not only in individuals or populations but potentially in ecosystems. Seabirds are among the most threatened marine taxonomic groups in the world, with ~25% of this species currently listed as threatened or considered of special concern; among seabirds, the penguins (Family Spheniscidae) are the most threatened seabird Family. We used gene expression to develop baseline physiological indices for wild penguins in the Falkland-Malvinas Islands, and captive zoo penguins. We identified the almost complete statistical separation of penguin groups (gentoo Detroit Zoo, gentoo Falkland-Malvinas Islands, rockhopper Detroit Zoo, and rockhopper Falkland-Malvinas Islands) based on gene expression profiles. Implementation of long-term longitudinal studies would allow for the assessment of temporal increases or decreases of select transcripts and would facilitate interpretation of the drivers of change.
Searching for Low Probability Opening Events in a DNA Sliding Clamp
Authors: Esmaeeli Reza , Andal Benedict , Perez Alberto
Published: February 2022

The β subunit of E. coli DNA polymererase III is a DNA sliding clamp associated with increasing the processivity of DNA synthesis. In its free form, it is a circular homodimer structure that can accomodate double-stranded DNA in a nonspecific manner. An open state of the clamp must be accessible before loading the DNA. The opening mechanism is still a matter of debate, as is the effect of bound DNA on opening/closing kinetics. We use a combination of atomistic, coarse-grained, and enhanced sampling strategies in both explicit and implicit solvents to identify opening events in the sliding clamp. Such simulations of large nucleic acid and their complexes are becoming available and are being driven by improvements in force fields and the creation of faster computers. Different models support alternative opening mechanisms, either through an in-plane or out-of-plane opening event. We further note some of the current limitations, despite advances, in modeling these highly charged systems with implicit solvent.
Barium 5-(tert-butyl)-2,3-dihydroxybenzenesulfonate
Authors: Rubicheva Lyubov G. , Lukyanov Daniil A.
Published: February 2022

Catechols and their derivatives attract great scientific interest due to the broad spectrum of their functional properties, including complexation, redox behavior, association ability and antioxidant activity. Because of the low molecular mass and two-electron redox process, they are considered to be a promising energy storage compound in different types of electrochemical power sources, such as metal-ion batteries or redox flow batteries. Herein, we report a preparation of the sterically hindered sulfonated catechol, namely the barium salt of 5-(tert-butyl)-2,3-dihydroxybenzenesulfonic acid, by the direct sulfonation of 4-tert-butylcatechol, by concentrated sulfuric acid. The proposed procedure is green and atom-economic, providing the desired product in high yield after simple purification. The solvent-free procedure is inexpensive and highly scalable, which enables direct industrial production of the title product. The resulting product was characterized by the 1H and 13C nuclear magnetic resonance (NMR) and ESI-high resolution mass spectrometry (ESI-HRMS).
Effect of Plasticizer Content on Mechanical and Water Vapor Permeability of Maize Starch/PVOH/Chitosan Composite Films
Authors: Caicedo Carolina , Díaz-Cruz Claudio Alonso , Jiménez-Regalado Enrique Javier , Aguirre-Loredo Rocio Yaneli
Published: February 2022

Packaging materials based on biodegradable polymers are a viable alternative to replace conventional plastic packaging from fossil origin. The type of plasticizer used in these materials affects their functionality and performance. The effect of different plasticizers such as glycerol (GLY), sorbitol (SOR), and poly(ethylene glycol) (PEG) in concentrations of 5%, 10%, and 15% (w/w) on the structural features and functional properties of starch/PVOH/chitosan films was evaluated. The incorporation of a plasticizer increased the thickness of the biodegradable composite films. Furthermore, the material plasticized with 30% (w/w) sorbitol had the highest elongation at break, lowest water vapor permeability, and better thermal resistance. The results obtained in this study suggest that maize starch/PVOH/chitosan biodegradable composite films are a promising packaging material, and that sorbitol is the most suitable plasticizer for this formulation.
Density Functional Theory Description of Paramagnetic Hexagonal Close-Packed Iron
Authors: Choi Youngwon , Dong Zhihua , Li Wei , Lizárraga Raquel , Kwon Se-Kyun , Vitos Levente
Published: February 2022

The hexagonal close-packed (hcp) phase of iron is unstable under ambient conditions. The limited amount of existing experimental data for this system has been obtained by extrapolating the parameters of hcp Fe–Mn alloys to pure Fe. On the theory side, most density functional theory (DFT) studies on hcp Fe have considered non-magnetic or ferromagnetic states, both having limited relevance in view of the current understanding of the system. Here, we investigate the equilibrium properties of paramagnetic hcp Fe using DFT modelling in combination with alloy theory. We show that the theoretical equilibrium c/a and the equation of state of hcp Fe become consistent with the experimental values when the magnetic disorder is properly accounted for. Longitudinal spin fluctuation effects further improve the theoretical description. The present study provides useful data on hcp Fe at ambient and hydrostatic pressure conditions, contributing largely to the development of accurate thermodynamic modelling of Fe-based alloys.
Synthesis and Characterization of High Surface Area Transparent SiOC Aerogels from Hybrid Silicon Alkoxide: A Comparison between Ambient Pressure and Supercritical Drying
Authors: Abebe Adane Muche , Soraru Gian Domenico , Thothadri Ganesh , Andoshe Dinsefa Mensur , Zambotti Andrea , Ahmed Gulam Mohammed Sayeed , Tirth Vineet , Algahtani Ali
Published: February 2022

In this article, highly porous and transparent silicon oxycarbide (SiOC) gels are synthesized from Bis(Triethoxysilyl) methane (BTEM). The gels are synthesized by the sol-gel technique followed by both ambient pressure and supercritical drying. Then, the portion of wet gels have been pyrolyzed in a hydrogen atmosphere at 800 and 1100 °C. The FT-IR spectroscopy analysis and nitrogen sorption results indicate the successful synthesis of Si-O-Si bonds and the formation of mesopores. From a hysteresis loop, the SiOC ceramics showed the H1 type characteristic with well-defined cylindrical pore channels for the aerogel and the H2 type for the ambigel samples, indicating that the pores are distorted due to the capillary stress. The produced gels are mesoporous materials having high surface areas with a maximum of 1140 m2/g and pore volume of 2.522 cm3/g obtained from BTEM aerogels. The pyrolysis of BTEM aerogels at 800 °C results in the production of a bulk and transparent sample with a slightly pale white color, while BTEM xerogels are totally transparent and colorless at the same temperature. At 1100 °C, all the aerogels become opaque brown, confirming the formation of free carbon and crystalline silicon.
Simulation and Experimental Study on Roll-Forming Limit of Cup
Authors: Zhang Baohong , Ning Shengpeng , Wei Zeng , Duan Yali , Li Xubin , Yang Yongbiao
Published: February 2022

Roll forming can improve the material utilization rate and production efficiency of cups with a curved rotary profile, but there is no basis for the determination of forming limit. The DEFORM-3D software was used to simulate the roll forming of cups. The influence of the billet wall thickness and bottom thickness, coefficient of friction, radius of roller, and the fillet radius of the punch on the forming limit was studied, and the damage value and velocity vector were analyzed. The results showed that the forming limit of the billet’s wall thickness in roll forming for a cup is about 62%. With the increase of the ratio of the formed cup’s wall thickness to the billet’s bottom thickness, the forming limit of wall thickness will be slightly reduced. A larger radius of roller, fillet radius of punch, and friction coefficient between punch and billet and a smaller friction coefficient between roller and billet are good for decreasing the damage value and improving the roll-forming limit. According to the numerical simulation results, the roll-forming limit diagram of cups is established, and the accuracy of the forming limit diagram is verified by experiments.
Physical and Mechanical Properties of Particleboard Produced with Addition of Walnut (Juglans regia L.) Wood Residues
Authors: Pędzik Marta , Auriga Radosław , Kristak Lubos , Antov Petar , Rogoziński Tomasz
Published: February 2022

The depletion of natural resources and increased demand for wood and wood-based materials have directed researchers and the industry towards alternative raw materials for composite manufacturing, such as agricultural waste and wood residues as substitutes of traditional wood. The potential of reusing walnut (Juglans regia L.) wood residues as an alternative raw material in particleboard manufacturing is investigated in this work. Three-layer particleboard was manufactured in the laboratory with a thickness of 16 mm, target density of 650 kg∙m−3 and three different levels (0%, 25% and 50%) of walnut wood particles, bonded with urea-formaldehyde (UF) resin. The physical properties (thickness swelling after 24 h) and mechanical properties (bending strength, modulus of elasticity and internal bond strength) were evaluated in accordance with the European standards. The effect of UF resin content and nominal applied pressure on the properties of the particleboard was also investigated. Markedly, the laboratory panels, manufactured with 50% walnut wood residues, exhibited flexural properties and internal bond strength, fulfilling the European standard requirements to particleboards used in load-bearing applications. However, none of the boards met the technical standard requirements for thickness swelling (24 h). Conclusively, walnut wood residues as a waste or by-product of the wood-processing industry can be efficiently utilized in the production of particleboard in terms of enhancing its mechanical properties.
Impact Resistance of Styrene–Butadiene Rubber (SBR) Latex-Modified Fiber-Reinforced Concrete: The Role of Aggregate Size
Authors: Ashraf Muhammad Rehan , Akmal Usman , Khurram Nauman , Aslam Fahid , Deifalla Ahmed F.
Published: February 2022

Improvements in tensile strength and impact resistance of concrete are among the most researched issues in the construction industry. The present study aims to improve the properties of concrete against impact loadings. For this purpose, energy-absorbing materials are used along with fibers that help in controlling the crack opening. A polymer-based energy-absorbing admixture, SBR latex, along with polypropylene fibers are used in this study to improve the impact resistance. Along with fibers and polymers, the effect of the size of aggregates was also investigated. In total, 12 mixes were prepared and tested against the drop weight test and the Charpy impact test. Other than this, mechanical characterization was also carried out for all the 12 concrete mixes. Three dosages of SBR latex, i.e., 0%, 4%, and 8% by weight of cement, were used along with three aggregates sizes, 19 mm down, 10 mm down, and 4.75 mm down. The quantity of polypropylene fibers was kept equal to 0.5% in all mixes. In addition to these, three control samples were also prepared for comparison. The mix design was performed to achieve a normal-strength concrete. For this purpose, a concrete mix of 1:1.5:3 was used with a water to a cement ratio of 0.4 to achieve a normal-strength concrete. The experimental study concluded that the addition of SBR latex improves the impact resistance of concrete. Furthermore, an increase in impact resistance was also observed for a larger aggregate size. The use of fibers and SBR latex is encouraged due to their positive results and the fact that they provide an economical solution for catering to impact strains. The study concludes that 4% SBR latex and 0.5% fibers with a larger aggregate size improve the resistance against impact loads.
Hand and Abrasive Flow Polished Tungsten Carbide Die: Optimization of Surface Roughness, Polishing Time and Comparative Analysis in Wire Drawing
Authors: Kumar Raman , Singh Sehijpal , Aggarwal Vivek , Singh Sunpreet , Pimenov Danil Yurievich , Giasin Khaled , Nadolny Krzysztof
Published: February 2022

This research work highlights the benefits of abrasive flow polishing (AFP) applied to tungsten carbide dies compared with conventional hand polishing (HP). An indigenous experimental set-up for AFP was developed. The effect of prominent process parameters viz. extrusion pressure, number of cycles, and abrasive particle concentration on the final surface roughness, percentage improvement in surface roughness, and polishing time was investigated by Taguchi-designed experiments. The multi-objective optimization (MOO) was performed using the Taguchi-TOPSIS-Equal weight approach to find the respective optimized AFP parametric settings. A set of skilled operators performed the conventional HP of dies, and the best hand-polished (HPed) die was selected using the TOPSIS technique. The operational performance of the HPed dies and the abrasive flow polished (AFPed) dies were compared on the three-stage wire drawing operation. The results revealed that AFP’s surface resulted in a better-quality surface than hand polishing with a 27.06% improvement in surface roughness. Furthermore, AFP can reduce the dependency on costly and tricky-to-locate skilled operators, with a reasonable amount of time saving (about 87.05%). Overall, the study’s findings show that abrasive flow polishing of dies is fast and cost-effective.
Effects of Strength and Distribution of SiC on the Mechanical Properties of SiCp/Al Composites
Authors: Wang Yanju , Wei Wei , He Xiaolei , Lan Xiang , Sha Aixue , Hao Wenfeng
Published: February 2022

In this paper, considering the strength and geometric discrete distribution characteristics of composite reinforcement, by introducing the discrete distribution function of reinforcement, the secondary development of ABAQUS is realized by using the Python language, the parametric automatic generation method of representative volume elements of particle-reinforced composites is established, and the tensile properties of silicon carbide particle-reinforced aluminum matrix composites are analyzed. The effects of particle strength, particle volume fraction, and particle random distribution on the mechanical properties of SiCp/Al composites are studied. The results show that the random distribution of particles and the change in particle strength have no obvious influence on the stress–strain relationship before the beginning of material damage, but have a great influence on the damage stage, maximum strength, and corresponding failure strain. With the increase in particle volume fraction, the damage intensity of the model increases, and the random distribution of particles has a great influence on the model with a large particle volume fraction. The results can provide a reference for the design, preparation, and characterization of particle-reinforced metal matrix composites.
On the Effects of Core Microstructure on Energy Absorbing Capabilities of Sandwich Panels Intended for Additive Manufacturing
Authors: Acanfora Valerio , Castaldo Rossana , Riccio Aniello
Published: February 2022

Increasing transportation safety can be observed as one of the biggest engineering challenges. This challenge often needs to be combined with the need to deliver engineering solutions that are able to lower the environmental impact of transportation, by reducing fuel consumption. Consequentially, these topics have attracted considerable research efforts. The present work aims to address the previously cited challenges by maximizing the energy absorption capabilities of hybrid aluminum/composite shock absorbers with minimal thickness and mass. This engineering solution makes it possible to lighten vehicles and reduce fuel consumption, without compromising safety, in terms of crashworthiness capabilities. A numerical sensitivity study is presented, where the absorbed energy/mass (AE/m) and the absorbed energy/total panel thickness (AE/Htot) ratios, as a consequence of low-velocity impact simulations performed on six different shock absorbers, are compared. These hybrid shock absorbers have been numerically designed by modifying the core thickness of two basic absorbers’ configurations, characterized, respectively, by a metallic lattice core, intended to be produced through additive manufacturing, and a standard metallic honeycomb core. This work provides interesting information for the development of shock absorbers, which should be further developed with an experimental approach. Indeed, it demonstrates that, by integrating composite skins with a very light core producible, by means of additive manufacturing capabilities, it is possible to design shock absorbers with excellent performance, even for very thin configurations with 6 mm thickness, and to provide a significant increase in AE/m ratios when compared to the respective equal volume standard honeycomb core configurations. This difference between the AE/m ratios of configurations with different core designs increases with the growth in volume. In detail, for configurations with a total thickness of 6 mm, the AE/m increases in additive manufacturing configurations by approximately 93%; for those with a total thickness of 10 mm, the increase is 175%, and, finally, for those with a total thickness of 14 mm, the increase is 220%.
Properties and Applications of Metal Phosphates and Pyrophosphates as Proton Conductors
Authors: Colodrero Rosario M. P. , Olivera-Pastor Pascual , Cabeza Aurelio , Bazaga-García Montse
Published: February 2022

We review the progress in metal phosphate structural chemistry focused on proton conductivity properties and applications. Attention is paid to structure–property relationships, which ultimately determine the potential use of metal phosphates and derivatives in devices relying on proton conduction. The origin of their conducting properties, including both intrinsic and extrinsic conductivity, is rationalized in terms of distinctive structural features and the presence of specific proton carriers or the factors involved in the formation of extended hydrogen-bond networks. To make the exposition of this large class of proton conductor materials more comprehensive, we group/combine metal phosphates by their metal oxidation state, starting with metal (IV) phosphates and pyrophosphates, considering historical rationales and taking into account the accumulated body of knowledge of these compounds. We highlight the main characteristics of super protonic CsH2PO4, its applicability, as well as the affordance of its composite derivatives. We finish by discussing relevant structure–conducting property correlations for divalent and trivalent metal phosphates. Overall, emphasis is placed on materials exhibiting outstanding properties for applications as electrolyte components or single electrolytes in Polymer Electrolyte Membrane Fuel Cells and Intermediate Temperature Fuel Cells.