bluuebunny/crossref_metadata_2025 huggingface dataset:
Streptococcus agalactiae isolated from clinical mastitis cases on large dairy farms in north China: phenotype, genotype of antimicrobial resistance and virulence genes
Authors: Liu Kai , Liu Xiang , Yang Jieyan , Gu Xiaolong , Zhang Limei , Qu Weijie
Published: September 2024

Streptococcus agalactiae (Strep. agalactiae) is bovine mastitis pathogen and has thus became a matter of concern to dairy farms worldwide in terms of economic loss. The aims of this study were to (a) determine virulence genes, and (b) characterize the antimicrobial resistance (AMR) profiles and AMR genes and (c) figure out the relationship between AMR phenotypes and genotypes of Strep. agalactiae isolated from dairy cows in north China. A total of 20 virulence genes and 23 AMR genes of 140 isolates collected from 12 farms in six provinces were studied. The antimicrobial susceptibility of 10 veterinary commonly used antimicrobials were tested using the broth microdilution method. Results showed that all the isolates harbored the virulence genes lacIV, gapC, and dltA. The isolates that harbored the genes lacIII, fbsA, hylB, and cfb exhibited the high prevalence (99.29%), followed by isolates that harbored lacI (98.57%), bibA (97.86%), cylE (97.14%), lacII (92.14%), cspA (52.14%), pavA (25%), bca (2.14%), and scpB (0.71%). The fbsB, lmb, spbI, bac, and rib genes were not detected. The virulence patterns of B (fbsA_cfb_cylE_ hylB_bibA_cspA_ gapC_dltA_lacIII/IV) and C (fbsA_cfb_ bibA _ gapC_ dltA_lacIV) were dominant, accounting for 97.86% of the isolates. The following AMR genes were prevalent: pbp1A (97.14%), tet(M) (95.00%), lnu (A) (80.71%), erm (B) (75.00%), tet(O) (72.14%), blaZ (49.29%), tet(S) (29.29%), blaTEM (25.71%), erm (A) (17.14%), erm (C) (13.57%), tet (L) (10.71%), linB (2.86%), and erm (TR) (2.86%). The pbp2b, mecA1, mecC, lnu (D), erm (F/G/Q), and mef (A) genes were not detected. Eighty percent of the isolates harbored AMR genes and were highly resistant to tetracycline, followed by macrolides (10.71%), lincosamides (9.29%) and β-lactams (4.29%). In conclusion, isolates only exhibited well correlation between tetracyclines resistance phenotype and genotype, and almost all isolates harbored intact combination of virulence genes.
Hypothyroidism and dermato/polymyositis: a two-sample Mendelian randomization study
Authors: Li Qianqian , Yang Shaoying , Ma Yan , Huang Huijing , Zhi Langxian , Wang Suli , Lu Liangjing
Published: September 2024

Objective

Observational studies have revealed a higher probability of hypothyroidism in patients with dermatomyositis (DM) or polymyositis (PM), but there is no consensus on whether hypothyroidism causally influences DM or PM. In the present study, we assessed the causal association between hypothyroidism and the risk of dermatomyositis or polymyositis using two-sample Mendelian randomization (TSMR).

Methods

The genome-wide association data of hypothyroidism and dermatomyositis/polymyositis were obtained from the IEU Open GWAS project. Then, TSMR was used to determine whether hypothyroidism is causally associated with DM or PM. Single-nucleotide polymorphisms (SNPs) significantly associated with hypothyroidism were identified and used as instrumental variables (IVs), and the causal relationship between hypothyroidism and DM/PM was examined using TSMR. MR pleiotropy and Cochran’s Q test were used to confirm the heterogeneity and pleiotropy of identified IVs, then four different models, including the inverse variance weighted model (IVW), MR-Egger, weighted median and weighted model were applied in this MR analysis.

Results

Sixty-eight SNPs for DM and 68 SNPs for PM were selected as the IVs (P<5×10−8; linkage disequilibrium R2 <0.001) to assess the causal association between hypothyroidism and DM/PM selected from GWASs on hypothyroidism. The results revealed a positive causal effect of hypothyroidism on both DM and PM (DM: OR 2.563, 95% CI [1.348, 4.874], P = 0.00156; PM: OR1.709, 95% CI [1.157, 2.525], P =0.007). Moreover, there was no heterogeneity or pleiotropy in the results.

Conclusion

In conclusion, the MR analysis results provided strong evidence to indicate that hypothyroidism might be causally associated with DM and PM. These findings may have important implications for the pathogenesis and possible future therapies of DM/PM.
Assessment of sociodemographic and psychiatric characteristics of transgender adults seen at a Midwest tertiary medical center
Authors: Cortez Samuel , Moog Dominic , Baranski Elizabeth , Williams Kelley , Wang Jinli , Nicol Ginger , Baranski Thomas , Herrick Cynthia J.
Published: September 2024

Background

The National Academy of Medicine has formally identified transgender adults as an understudied population in critical need of health research. While national surveys, like the US Transgender survey, have characterized higher rates of depression, anxiety, suicidality and socioeconomic need in the transgender community, studies have not examined the impact of sociodemographic and clinical characteristics on mental health related outcomes.

Objective

To describe the sociodemographic and mental health characteristics of transgender adults seen at a large Midwest transgender clinic and to determine factors associated with self-reported mental health conditions.

Methods

Descriptive, retrospective, cross-sectional study of new transgender patients 18 years and older seen at a large Midwest transgender clinic between December 2019 and June 2022.

Results

A total of 482 charts were reviewed. During their initial evaluation, 11.6% (56/482) reported having a history of suicide attempt and 81.3% (392/482) reported a mental health diagnosis with the most common being depression, anxiety, attention deficit disorder, and post-traumatic stress disorder. Multivariable logistic regression results show no single factor was significantly associated with mental health diagnosis after adjusting for the effect of age and race. Patients who were new to gender affirming hormone therapy (54%, 254/468) are 2.0 (95% CI 1.4-2.9) times more likely to report having a mental health care provider than patients who were seen for continuation of therapy (46%, 214/468). Ten records with race not disclosed, 3 records with gender identity “other” and 2 records with gender identity not disclosed were excluded from analysis.

Conclusion

This study reinforces the finding that transgender adults have an increased lifetime prevalence of mental health conditions. The higher prevalence of mental health conditions in our clinic was not associated with sociodemographic factors included in the study. Furthermore, transgender patients are less likely to have seen mental healthcare providers after initiation of gender affirming hormone therapy.
Metabolic-associated steatotic liver disease and risk of Alzheimer’s disease: a real-world retrospective cohort study
Authors: Zhang Jiaqi , Wang Wenzhao , Hou Xingyun , Wu Jia , Wang Yifan , Fan Jianling , Zhang Zhiyu , Yuan Zhizhong , Sun Cuifen , Lu Bin , Zheng Jiaoyang
Published: September 2024

Objective

Alzheimer’s Disease (AD) is increasingly recognized as being associated with metabolic disorders, including Metabolic Associated Steatotic Liver Disease (MASLD). This study aimed to assess the relative risk of AD in individuals with MASLD.

Methods

In this retrospective cohort study, we analyzed data from individuals aged over 65 who underwent health check-ups between January 2018 and June 2023. MASLD was diagnosed based on ultrasound findings and cardiometabolic criteria. AD incidence was identified using ICD-10 codes and self-reports. Poisson regression models estimated the relative risk of AD in relation to MASLD, adjusting for age, BMI, sex, SBP, HbA1c, HDL-c, triglycerides, hs-CRP, GGT, and estimated GFR.

Results

The study included 4,582 MASLD patients and 6,318 controls. MASLD patients showed a higher incidence of AD (127 cases) compared to controls (61 cases). The fully adjusted Poisson regression model indicated an increased AD risk in MASLD patients [RR: 2.80 (95% CI: 1.79-4.38)].

Conclusion

Our findings suggested MASLD as an independent risk factor for AD, underlining the role of metabolic dysfunctions in AD pathogenesis. The study emphasized the need for comprehensive metabolic health management in AD prevention strategies, particularly among high-risk groups.
N6-methyladenine RNA methylation epigenetic modification and diabetic microvascular complications
Authors: Wang Yuanyuan , Zou Jiayun , Zhou Hua
Published: September 2024

N6-methyladensine (m6A) has been identified as the best-characterized and the most abundant mRNA modification in eukaryotes. It can be dynamically regulated, removed, and recognized by its specific cellular components (respectively called “writers,” “erasers,” “readers”) and have become a hot research field in a variety of biological processes and diseases. Currently, the underlying molecular mechanisms of m6A epigenetic modification in diabetes mellitus (DM) and diabetic microvascular complications have not been extensively clarified. In this review, we focus on the effects and possible mechanisms of m6A as possible potential biomarkers and therapeutic targets in the treatment of DM and diabetic microvascular complications.
Exploring the spatiotemporal dynamics and coupling coordination of urbanization and habitat quality in the Yangtze river basin, China
Authors: Wu Jianfeng , Guo Zhongyang , Luo Guangjie , Zhang Fengtai , Chen Jingpeng , Li Wei , Chen Qiwei , Liao Jingjing , Cao Guangjie
Published: September 2024

Rapid urbanization induces significant changes in land use, exerting a profound impact on regional habitat quality. Understanding the interconnectedness of urbanization and habitat quality is crucial for safeguarding the regional ecological environment and promoting sustainable urban development. Cities along the Yangtze River, as the core region of the Yangtze River Economic Belt in China, serve as a typical and critically important area to investigate the dynamic response of habitat quality to rapid urbanization. This study employs the InVEST model and entropy method to assess the spatiotemporal dynamics of habitat quality and urbanization in Yangtze River cities. Using the coupling coordination degree model, it analyzes the interplay between urbanization and habitat quality, elucidating dynamic response patterns and emphasizing the need for pertinent policy measures. Our findings reveal that from 1990 to 2019, urbanization levels in the study area surged by over six times, while habitat quality consistently declined, reflecting a 4.5% decrease. Notably, spatial variances were observed along the upstream, midstream, and downstream segments of the river. Although an inverse relationship between urbanization and habitat quality predominates, three cities demonstrated a positive correlation. The study also indicates a stable increase in the coupling coordination degree between urbanization and habitat quality, evolving from a phase of urbanization lag to one of synchronization followed by habitat quality lag. Among the cities, Chongqing emerges as an exemplary model for the coordinated development of urbanization and habitat quality. These insights provide a theoretical foundation and policy guidance for enhancing sustainable urban and ecological strategies in the region.
A blueprint for overcoming barriers to the use of nature-based coastal protection in Australia
Authors: Morris Rebecca L. , Pomeroy Andrew W. M. , Boxshall Anthony , Colleter Gildas , Dack David , Dunlop Andrew R. , Hanslow David , King Sam , Magini Ariana , O’Malley-Jones Katrina , Sultmann Sel , Townsend Murray , Valesini Fiona , White Jacquie , Zavadil Elisa , Swearer Stephen E.
Published: September 2024

The global loss of coastal habitats is putting communities at risk of erosion and flooding, as well as impacting ecosystem function, cultural values, biodiversity, and other services. Coastal habitat restoration can provide a nature-based solution to the increasing need for climate adaptation on the coast while recovering lost ecosystems. Despite the benefits of using nature-based coastal protection to manage coastal hazards, there are scientific, socio-political and economic barriers to the broad use of this approach. Understanding the details of these barriers from the perspective of multiple stakeholders is essential to identifying solutions to overcome them. Using a workshop with participants that are key partners and stakeholders (from government, engineering consulting firms, and non-governmental organisations) in the management, design, and delivery of a coastal protection solution we aimed to: (1) gain a better understanding of the barriers faced by multiple stakeholders involved in the implementation of nature-based coastal protection; and (2) identify tangible solutions to these barriers to increase or support implementation, help focus attention on areas for future research, and inform pathways forward for the governance of nature-based coastal protection. We defined 19 barriers to nature-based coastal protection, but the primary ones that are experienced during the delivery of a project are a lack of: education and awareness; community support; necessary expertise and technical guidance; and uncertainty around: the risk reduction that can be achieved; planning and regulatory processes; and ownership of the structure. Two barriers that do not persist during the design stages of a project but are overarching as to whether nature-based coastal protection is considered in the first place, are government support and the availability of funding. The importance of these primary barriers changes depending on the method of nature-based coastal protection. We conclude by identifying both immediate actions and long-term solutions for enabling nature-based coastal protection in response to each of the primary barriers.
Playback-elicited heterospecific aggressive responses in urbanized Sparkling Violetear are modulated in relation the risk of take-over hypothesis
Authors: Tellería Luciana , Garitano-Zavala Álvaro
Published: September 2024

Hummingbirds have crucial ecological importance in natural and human-altered habitats in the Neotropics. Their unique biological characteristics imply a delicate energetic balance that drove the evolution of species-specific dominance and territorial behavior tactics that, in turn, shape the diversity and composition of nectarivorous communities. Understanding these factors could help improve conservation strategies, particularly important for eroding communities in cities. Our objective is to evaluate whether a species of territorial hummingbird, the Sparkling Violetear (Colibri coruscans), is able to modulate its aggressive behavior in relation to the identity of the species that invades its feeding territory, comparing between dry (relative depletion of nectar resources) and wet seasons, and analyzing the possible ecological factors that determine this response. Considering the maximization of energy efficiency, we hypothesize that the territorial aggressive responses of Sparkling Violetears will vary in relation to the territoriality and behavioral dominance of each intruder species, and that aggressive responses in the dry season will be greater compared to the wet season. We elicited aggressive behavioral responses with territorial songs playbacks from the four species that compose the urban nectarivorous bird community, including songs from their own species, characterizing eight behaviors that varied in aggressive intensity. We quantified the aggressive response in two ways: the number of observation events in which territorial Sparkling Violetears performed each behavior and by constructing an “aggressiveness score” for each territorial individual in each observation event. Territorial aggressive response varied significantly in relation to the identity of the intruding species, but the seasonal effect was only observed, as a more aggressive response in the dry season, towards heterospecific playbacks. We analyzed several hypotheses that could explain the species-specific aggressive response, concluding that the “risk to take-over” hypothesis, through wing morphology and maneuverability, best explains the modulation of the aggressive response in relation to the territoriality and behavioral dominance of each intruder species. These results are useful for urban planning if we elucidate the ecological conditions that could promote the coexistence of subordinate species with dominant ones.
Genetic association and causal effects between inflammatory bowel disease and conjunctivitis
Authors: Chang Shuangqing , Luo Qinghua , Huang Zhifang
Published: September 2024

Background

Inflammatory bowel disease (IBD) is often clinically associated with conjunctivitis, which may result from genetic associations and causal effects.

Methods

Genetic correlations were investigated through the genome-wide association study (GWAS) data on IBD and conjunctivitis using the linkage disequilibrium score regression (LDSC) and heritability estimated in summary statistics (HESS). The causal effect analysis was performed using four methods of Mendelian randomization (MR) and the genetic risk loci common to both diseases were identified by the statistical method of conditional/conjoint false discovery rate (cond/conjFDR), followed by genetic overlap analysis. Finally, a multi-trait GWAS analysis (MTAG) was performed to validate the identified shared loci.

Results

IBD (including CD and UC) and conjunctivitis showed a significant overall correlation at the genomic level; however, the local correlation of IBD and CD with conjunctivitis was significant and limited to chromosome 11. MR analysis suggested a significant positive and non-significant negative correlation between IBD (including CD and UC) and conjunctivitis. The conjFDR analysis confirmed the genetic overlap between the two diseases. Additionally, MTAG was employed to identify and validate multiple genetic risk loci.

Conclusion

The present study provides evidence of genetic structure and causal effects for the co-morbidity between IBD (both CD and UC) and conjunctivitis, expanding the epidemiologic understanding of the two diseases.
Size dependence on shear fatigue and fracture behavior of ball grid array structure Cu/Sn–3.0Ag–0.5Cu/Cu solder joints under current stressing
Authors: Wang Bo , Li Wangyun , Pan Kailin , Huang Wei , Gong Yubing
Published: September 2024

The shear fatigue performance and fracture behavior of microscale ball grid array (BGA) structure Cu/Sn-3.0Ag-0.5Cu/Cu solder joints with different heights (500 μm, 300 μm, and 100 μm) with increasing current density (from 6.0 × 103 to 1.1 × 104 A/cm2) were investigated systematically. The results reveal that the fatigue life of solder joints decreases with increasing current density, while increasing with decreasing the height of solder joints. The location of fatigue fracture shifts from solder matrix to the interface between solder and intermetallic compound (IMC) layer for those with heights of 500 μm and 300 μm with increasing current density, in which the interfacial fracture is triggered by current crowding at the groove of the IMC layer and driven by mismatch strain at the solder/IMC layer interface; while, the fatigue fracture all occurs in the solder matrix for solder joints with a height of 100 μm. Moreover, the fracture in solder matrix of solder joints with heights of 500 μm and 300 μm exhibits an arc-shape fracture path, while a linear path for those with a height of 100 μm. These fracture paths are consistent with the concentration distribution region of plastic strain energy in solder joints.
How do cultural elements shape speak-up behavior beyond the patient safety context? An interprofessional perspective in an obstetrics and gynecology department
Authors: Malik Romana F. , Azar Poyan , Taimounti Achraf , Buljac-Samardžić Martina , Hilders Carina G. J. M. , Scheele Fedde
Published: September 2024

Introduction

Interprofessional working and learning thrives with speak-up behavior. Efforts to improve speak-up have mainly focused on isolated techniques and training programs within the patient safety scope, yet sustained improvement requires a cultural shift beyond this scope. This research investigates the influence of culture elements on speak-up behavior in interprofessional teams beyond the patient safety context.

Methods

An exploratory qualitative study design was used in a Dutch hospital’s Obstetrics and Gynecology department. A representative sample of stakeholders was purposefully selected, resulting in semi-structured interviews with 13 professionals from different professional backgrounds (nurses, midwifes, managers, medical specialists, and residents). A speak-up pledge was developed by the research team and used to prime participants for discussion. Data analysis involved three-step coding, which led to the development of themes.

Results

This study has identified six primary cultural themes that enhance speak-up behavior. These themes encompass the importance of managing a shared vision, the role of functional hierarchy, the significance of robust interpersonal relationships, the formulation of a strategy delineating when to speak up and when to exercise restraint, the promotion of an open-minded professional mindset, and the integration of cultural practices in the context of interprofessional working and learning.

Conclusion

Six crucial cultural elements have been pinpointed to boost the practice of speaking up behavior in interprofessional working and learning. Remarkably, hierarchy should not be held responsible as the wrongdoer; instead, can be a great facilitator through respect and appreciation. We propose that employing transformational and humble leadership styles can provide guidance on effectively integrating the identified cultural elements into the workplace and provide an IMOI framework for effective interprofessional speak-up beyond patient safety.
Disability health in medical education: development, implementation, and evaluation of a pilot curriculum at Stanford School of Medicine
Authors: Sapp Richard W. , Lee Edmund , Bereknyei Merrell Sylvia , Schillinger Erika , Lau James N. , Feldman Heidi M. , Poffenberger Cori McClure
Published: September 2024

Background

People with disabilities face significant healthcare disparities due to barriers to accessing care, negative attitudes of providers, and lack of education on disabilities for healthcare professionals. Physicians report discomfort when interacting with patients with disabilities, adding to the disparity, warranting research on medical school education.

Objective

Two educational interventions were structured: (1) a brief 2-h intervention in the mandatory curriculum and (2) a 9-week elective course which included interactions with individuals with disabilities through workshops and partner programs. We predicted that both of these interventions would result in improvements in attitude and empathy toward individuals with disabilities and reduce student anxiety.

Methods

During the 2018–2019 academic year, 54 students completed the surveys for the 2-h intervention and 8 students completed the 2-h intervention and elective course. Pre-, post-, and delayed post-intervention surveys (3 months after post survey) measured students’ attitudes, using validated surveys on attitudes, empathy and anxiety toward individuals with disabilities.

Results

Both educational interventions resulted in improved attitudes toward individuals with disabilities. However, students reported only feeling prepared to care for patients with disabilities after the elective course. The elective course, but not the 2-h course, significantly decreased student anxiety levels, likely due to more individual time working with individuals with disabilities. Delayed analysis after 3 months showed that both interventions had a lasting impact on attitudes and behavior change when caring for individuals with disabilities.

Conclusion

Medical education is effective at improving medical students’ attitudes and behaviors toward individuals with disabilities. A 2-h session can lead to a modest improvement in attitudes. However, more dedicated time and exposure to persons with disabilities results in a greater improvement in students’ attitudes, anxiety and preparedness.
Case series report: use of vibroacoustic pulmonary therapy in patients with thoracic trauma complicated by acute respiratory failure
Authors: Konkayev Aidos , Bekniyazova Assema , Khamidullina Zaituna , Konkayeva Maiya
Published: September 2024

Chest injury is often accompanied by polytrauma and is complicated by respiratory failure. This article presents a series of cases with verified acute respiratory failure in patients with chest injury, where vibroacoustic pulmonary therapy was used in complex treatment. Dynamic X-rays and respiratory drive indicators reflected the effect of the use of vibroacoustic lung therapy. Early diagnosis of respiratory disorders and complex therapy using vibroacoustic pulmonary therapy can improve results. As a result, the time spent in the hospital and in the intensive care unit is reduced, and the frequency of adverse outcomes is reduced.
Quantitative assessment and genomic profiling of Campylobacter dynamics in poultry processing: a case study in the United Arab Emirates integrated abattoir system
Authors: Habib Ihab , Mohamed Mohamed-Yousif Ibrahim , Lakshmi Glindya Bhagya , Al Marzooqi Hassan Mohamed , Afifi Hanan Sobhy , Shehata Mohamed Gamal , Khan Mushtaq , Ghazawi Akela , Abdalla Afra , Anes Febin
Published: September 2024

In the United Arab Emirates, no previous research has investigated the dynamics of the foodborne pathogen Campylobacter in broiler abattoir processing. This study conducted in one of the largest poultry producers in the UAE, following each key slaughter stage—defeathering, evisceration, and final chilling—five broiler carcasses were collected from 10 slaughter batches over a year. Additionally, one caecum was obtained from 15 chickens in each slaughter batch to evaluate the flock colonization. In total, 300 samples (150 carcasses and 150 caeca) were collected and enumerated for Campylobacter using standard methods. Campylobacter was pervasive in caecal samples from all slaughter batches, with 86% of carcasses post-defeathering and evisceration stages and 94% post-chilling tested positive for Campylobacter. Campylobacter coli predominates in 55.2% of positive samples, followed by Campylobacter jejuni in 21%, with both species co-existing in 23.8% of the samples. Campylobacter counts in caecal contents ranged from 6.7 to 8.5 log10 CFU/g, decreasing post-defeathering and evisceration to 3.5 log10 CFU/g of neck skin and further to 3.2 log10 CFU/g of neck skin post-evisceration. After chilling, 70% of carcasses exceeded 3 log10 CFU/g of neck skin. Whole-genome sequencing (WGS) of 48 isolates unveiled diverse sequence types and clusters, with isolates sharing the same clusters (less than 20 single nucleotide polymorphisms) between different farms, different flocks within the same farm, as well as in consecutive slaughter batches, indicating cross-contamination. Multiple antimicrobial resistance genes and mutations in gyrA T86I (conferring fluoroquinolone resistance) and an RNA mutation (23S r.2075; conferring macrolide resistance) were widespread, with variations between C. coli and C. jejuni. WGS results revealed that selected virulence genes (pglG, pseD, pseI, flaA, flaB, cdtA, and cdtC) were significantly present in C. jejuni compared to C. coli isolates. This study offers the first insights into Campylobacter dynamics in poultry processing in the UAE. This work provides a base for future research to explore additional contributors to Campylobacter contamination in primary production. In conclusion, effective Campylobacter management demands a comprehensive approach addressing potential contamination sources at every production and processing stage, guided by continued microbiological surveillance and genomic analysis to safeguard public health and food safety.
Continuous locomotor activity monitoring to assess animal welfare following intracranial surgery in mice
Authors: Abdollahi Nejat Mazyar , Stiedl Oliver , Smit August B. , van Kesteren Ronald E.
Published: September 2024

Locomotor activity can serve as a readout to identify discomfort and pain. Therefore, monitoring locomotor activity following interventions that induce potential discomfort may serve as a reliable method for evaluating animal health, complementing conventional methods such as body weight measurement. In this study, we used the digital ventilated cage (DVC®) system for the assessment of circadian locomotor activity, in addition to body weight monitoring, following intracranial stereotaxic surgery in an Alzheimer’s disease mouse model (C57BL/6J/APPswe/PSEN1dE9). Stereotaxic surgery did not affect the organization of circadian locomotor activity of both 7–8-week-old and 19–21-week-old mice. However, we observed that both young and old mice exhibited a significant decrease in activity during the dark phase. Also, our study shows that changes in locomotor activity exhibit higher sensitivity in detecting alterations indicative of animal health compared to measuring body weight. In contrast to 7–8-week-old mice, where we observed no genotypic differences in locomotor activity, 19–21-week-old APP/PS1 mice showed increased locomotor activity compared to wild-type mice. Furthermore, our analyses revealed that a subset of the 7–8-week-old mice showed increased locomotor activity during the initial peak of the dark phase. One mouse experienced sudden death early in life, possibly due to epileptic seizures. Altogether, our findings affirm continuous activity measurements as used in the DVC® as a highly valuable objective method for post-surgical welfare monitoring. Its discerning capacity not only facilitates circadian locomotor rhythm assessment but also enables the identification of individual aberrant activity patterns, possibly indicative of epileptic seizures.
Sudden sensorineural hearing loss: audiological profile during the COVID-19 pandemic
Authors: Peron Kelly Abdo , Scott Marina Cançado Passarelli , Soeiro Tracy Lima Tavares , do Amaral Jônatas Bussador , Chandrasekhar Sujana S. , de Oliveira Penido Norma
Published: September 2024

Introduction

Sudden sensorineural hearing loss (SSNHL) is an otological emergency that requires prompt recognition and intervention to prevent devastating impacts on people’s lives. During the COVID-19 pandemic, sensory deprivations have been reported in patients positive for SARS-CoV-2 virus, including deleterious effects on the auditory pathway. This study aims to describe the audiological profile of individuals with SSNHL during the COVID-19 pandemic and to correlate hearing recovery in subgroups of individuals with or without COVID-19.

Methods

Prospective cohort including patients diagnosed with SSNHL evaluated in a tertiary care center between March 2020 and September 2022. Hearing loss was confirmed with pure-tone and speech audiometry, with Speech Recognition Threshold (SRT) and word recognition score (WRS). Audiometric testing was performed at the moment of diagnosis, then 7, 30 and 120 days after diagnosis. The average degree of hearing loss was assessed by calculating the 4-frequency pure tone average (4fPTA). The investigation of COVID-19 included RT-PCR technique for the SARS-CoV-2 virus and collection of information regarding disease severity. A statistical analysis was performed using an analysis of covariance (ANCOVA) model to compare the 4fPTA between the four groups (with and without a history of COVID-19, unilateral and bilateral cases) at the end of the follow-up period.

Results

Fifty-two patients with SSNHL were assessed, 40 (76.9%) with unilateral and 12 (23.1%) with bilateral hearing loss, totaling 64 ears included. Of those, 15 (28.8%) patients tested positive for SARS-CoV-2 and were symptomatic for COVID-19. Of all unilateral cases, 22.5% were seropositive and showed symptoms of COVID-19, a number that increased to 50% for bilateral cases. Comparing the COVID-19 positive groups, individuals with unilateral SSNHL went from 40 dB as their average 4fPTA at onset to 20 dB as their average 4fPTA after 120 days, whereas those with bilateral SSNHL went from an initial average of 60 dB to a final average of 66 dB. Although the 4fPTA value of individuals with unilateral SSNHL improved in 7 days, the mean values showed no significant difference between positive and negative groups. There was a higher incidence of bilateral simultaneous SSNHL in patients who had not been vaccinated against COVID-19 and who presented with symptoms of severe COVID-19.

Conclusion

Infection with SARS-CoV-2 resulted in more severe SSNHL, in bilateral SSNHL, and in poorer recovery from SSNHL in bilateral cases. Bilateral SSNHL was seen more frequently in patients who had not received vaccination against COVID-19.
Therapeutic ketogenic diet as treatment for anorexia nervosa
Authors: Frank Guido K. W. , Scolnick Barbara
Published: September 2024

Anorexia nervosa (AN) is a severe psychiatric disorder. However, we lack neurobiological models and interventions to explain and treat the core characteristics of food restriction, feeling fat, and body size overestimation. Research has made progress in understanding brain function involved in the pathophysiology of AN, but translating those results into biological therapies has been challenging. Studies have suggested that metabolic factors could contribute to developing and maintaining AN pathophysiology. Here, we describe a neurobiological model for why using a therapeutic ketogenic diet could address key alterations in brain function in AN and prevent the desire for weight loss and associated eating disorder-specific symptoms. This translational model is based on animal studies and human data and integrates behavioral traits, brain neural energy metabolism, and neurotransmitter function. Pilot data indicate that the intervention can dramatically reduce eating and body-related fears, although larger studies across illness stages still need to be conducted.
Serum folate levels and risk of metabolic dysfunction-associated steatotic liver disease: results from a cross-sectional study and Mendelian randomization analysis
Authors: Chen Yalan , Gao Jie , Wang Xibin , Lu Hong , Zheng Ya , Ren Qian
Published: September 2024

Background

Evidence from observational studies on the association between folate and metabolic dysfunction-associated steatotic liver disease (MASLD) is conflicting.

Aims

This study aimed to investigate the association between serum folate concentration and MASLD and further assess the causal relationship using Mendelian randomization (MR) analysis.

Methods

To investigate the causal relationship between serum folate and MASLD, we conducted a cross-sectional study that selected 1,117 participants from the 2017–2020 National Health and Nutrition Examination Survey (NHANES). The association between serum folate level and the risk of MASLD was evaluated under a multivariate logistic regression model. In addition, we conducted a two-sample MR study using genetic data from a large genome-wide association study (GWAS) to compare serum folate level (37,465 individuals) and MASLD (primary analysis: 8,434 cases/770,180 controls; Secondary analysis:1,483 cases/17,781 controls) were performed to infer causal relationships between them. Inverse variance weighted (IVW) was used as the primary method of MR Analysis.

Results

The results from the NHANES database showed that Tertile 3 group (Tertile 3: ≥ 48.6 nmol/L) had a significantly lower risk (OR = 0.58, 95% CI: 0.38–0.88, p = 0.010) of MASLD than Tertile 1 group (Tertile 1: < 22.3 nmol/L) after complete adjustments. However, in the IVW of MR analysis, there was no causal relationship between serum folate level and MASLD risk in the primary analysis (OR = 0.75, 95% CI: 0.55–1.02, p = 0.065) and secondary analysis (OR = 0.83, 95% CI: 0.39–1.74, p = 0.618).

Conclusion

In observational analyses, we observed an inverse association between higher serum folate concentrations and a reduced risk of MASLD. Our MR study generated similar results, but the association failed to reach the significance threshold of p < 0.05, suggesting that our MR study does not support a causal relationship between serum folate levels and MASLD risk. Additional research involving a larger number of cases would contribute to enhancing the confirmation of our preliminary findings.
Manufacturing processes, additional nutritional value and versatile food applications of fresh microalgae Spirulina
Authors: Luo Guanghong , Liu Haiyan , Yang Shenghui , Sun Zhongliang , Sun Liqin , Wang Lijuan
Published: September 2024

Spirulina is capable of using light energy and fixing carbon dioxide to synthesize a spectrum of organic substances, including proteins, polysaccharides, and unsaturated fatty acids, making it one of the most coveted food resources for humanity. Conventionally, Spirulina products are formulated into algal powder tablets or capsules. However, the processing and preparation of these products, involving screw pump feeding, extrusion, high-speed automation, and high-temperature dewatering, often result in the rupture of cell filaments, cell fragmentation, and the unfortunate loss of vital nutrients. In contrast, fresh Spirulina, cultivated within a closed photobioreactor and transformed into an edible delight through harvesting, washing, filtering, and sterilizing, presents a refreshing taste and odor. It is gradually earning acceptance as a novel health food among the general public. This review delves into the manufacturing processes of fresh Spirulina, analyzes its nutritional advantages over conventional algal powder, and ultimately prospects the avenues for fresh Spirulina’s application in modern food processing. The aim is to provide valuable references for the research and development of new microalgal products and to propel the food applications of microalgae forward.
The effect of consuming nuts on cognitive function: a systematic review and meta-analysis of randomized clinical trials
Authors: Moabedi Mahdi , Aliakbari Mohammadreza , Erfanian Shima , Jibril Aliyu Tijani , Milajerdi Alireza
Published: September 2024

Background

Results from clinical trials investigating the effect of nuts consumption on cognition are conflicting. We decided to conduct the current meta-analysis to summarize all available evidence on the effect of consuming nuts on cognition scores.

Methods

We conducted a comprehensive search in the online databases using relevant keywords up to June 2024. We included all the published Randomized clinical trials (RCTs) investigating the effect of nuts, compared to control, on cognition scores.

Results

Overall, 5 trials were included with a total sample size of 928 adults. Based on 6 effect sizes from these 5 trials, we did not find a significant effect of nuts on cognition function [Standardized Mean Difference (SMD): 0.27, 95% CI: −0.65 to 1.19, p = 0.57].

Conclusion

Our review could not find a significant effect of nuts on cognition function. Future high-quality RCTs with larger sample sizes should be conducted to shed light on the impact of nuts on cognition.
Intracorporeal urinary diversion offers the advantage of delaying postoperative renal function injury in patients undergoing robot-assisted radical cystectomy
Authors: Wang Hao , Wang Wendi , Wang Xun , Fang Changhua , Zhao Kangkang , Chen Tianyi , Zhang Chengwei , Zhang Shiwei , Guo Hongqian , Zhang Gutian
Published: September 2024

Objective

To analyze changes in renal function and associated risk factors in patients with bladder cancer undergoing robot-assisted radical cystectomy (RARC) with intracorporeal or extracorporeal urinary diversion (ICUD or ECUD).

Methods

Clinical-pathological data was extracted from electronic medical records of 266 patients with bladder cancer who underwent RARC at our institution between August 2015 and August 2022. Postoperative renal function was assessed using the estimated glomerular filtration rate (eGFR).

Result

Patients were classified into ECUD and ICUD groups based on the surgical approach. Significant differences in eGFR were observed between the two groups at 1, 2, and 3 years postoperatively. Moreover, 112 patients (42.1%) experienced long-term renal function injury. Independent risk factors for long-term renal function injury included the type of surgical approach, ureteroenteric anastomotic strictures, and pathological stage T3 or above. In terms of short-term renal function, 30 cases of acute kidney injury (AKI) were observed, with an incidence rate of 11.3%. No difference in AKI incidence was found between the groups.

Conclusions

Postoperative AKI and chronic kidney injury are prevalent complications following RC. This study highlights that pathological stage, ureteroenteric anastomotic strictures, and ECUD significantly impact long-term renal function, but the type of urinary diversion (ileal conduit or orthotopic neobladder) had no effect on renal function, and ICUD was superior in terms of long-term renal injury rate. Therefore, precise preoperative assessment and the selection of appropriate surgical approach are crucial for preserving renal function in patients with bladder cancer.
Terlipressin versus placebo or noradrenalin in the treatment of hepatorenal syndrome: a systematic review and meta-analysis
Authors: Wan Yue-Meng , Huang Song-Quan , Wu Hua-Mei , Li Yu-Hua , Yin Hong-Jing , Xu Ying
Published: September 2024

Background

Hepatorenal syndrome (HRS) bears a very poor prognosis with unmet need for safe and effective therapies. This systematic review and meta-analysis aimed to re-assess safety and efficacy of terlipressin versus placebo or noradrenaline for HRS, based on previous randomized controlled trials (RCTs).

Methods

PubMed, EMBASE, MEDLINE (OvidSP) and Cochrane registers were searched for trials reporting HRS treatment by terlipressin or noradrenaline. Search terms included: “hepatorenal syndrome”, “terlipressin”, “noradrenaline”, and corresponding synonyms. Comparisons between terlipressin, noradreanaline, placebo and albumin were included. Meta-analysis was conducted for treatment response (both HRS reversal and complete response), mortality and adverse events.

Results

15 RCTs were included, enrolling 1236 HRS patients (type 1: 1166, type 2: 70). Treatment with terlipressin+albumin resulted in significantly higher treatment response than placebo+albumin or albumin alone (risk ratio [RR]:2.75, 95% confidence interval [CI]:1.96 to 3.84; I2 = 28%, p = 0.23; n = 6). Noradrenaline was equally effective in treatment response compared to terlipressin (RR:1.19, 95% CI:0.96 to 1.46; I2 = 16%, p = 0.31; n = 7), but trials were limited by its non-blind design and small size. Sensitivity analysis showed no survival benefit with terlipressin compared to either placebo (RR:1.03, 95% CI:0.83 to 1.28; I2 = 0%, p = 0.72; n = 3) or noradreanline (RR:0.83, 95% CI:0.69 to 1.00; I2 = 4%, p = 0.39; n = 7) at 30 days of follow-up. Terlipressin carried higher risk of treatment-related adverse events compared to either placebo (RR:2.92, 95% CI:1.48 to 5.77; I2 = 0%, p = 0.75; n = 3) or noradrenaline (RR:2.45, 95% CI:1.37 to 4.37; I2 = 0%, p = 0.92; n = 5).

Conclusion

Terlipressin is superior to placebo, and comparable to noradreanline in treatment response, but survival benefit is lacking. Noradrenaline, with low certainty, may be a better alternative for HRS.
Endogenous bufadienolides, mineralocorticoid receptor antagonists and fibrosis in chronic kidney disease
Authors: Rosenberg Mai , Bagrov Alexei Y.
Published: September 2024

Every year millions die prematurely of complications related to chronic kidney disease (CKD). Main causes of death are connected with cardiovascular (CV) complications. There is no cure for CKD although current treatment can slow the progression of the disease if diagnosed early. Fortunately, last decades have witnessed an accelerating pace of discovery regarding the cellular and molecular basis for CKD and CV disease. Novel biomarkers, including amino-terminal type III procollagen peptide (PIIINP), carboxy-terminal type I procollagen peptide (PICP), FGF23, marinobufagenin, and several miRNAs, show promise for early detection and risk stratification. In this review, we provide an overview of recent advances in the “fibrotic concept” of the etiology and pathogenesis of CKD which involves system consisting of Na/K-ATPase and its endogenous ligands including marinobufagenin which inhibits Fli1 and stimulates synthesis of collagen-1 in the vasculature. A novel treatment of CKD already involves the use of mineralocorticoid receptor antagonists capable of impairing marinobufagenin-Na/K-ATPase interactions.
Case report: Therapeutic drug monitoring and CYP2D6 phenoconversion in a protracted paroxetine intoxication
Authors: Damborská Alena , Hanáková Lenka , Pindurová Eva , Horská Kateřina
Published: September 2024

Objective

The cytochrome P450 2D6 (CYP2D6) is an enzyme involved in the oxidative biotransformation of various widely used drugs, including paroxetine, a substrate and strong inhibitor of the enzyme. The aim is to report on a case of protracted intoxication with paroxetine after a single overdose in a genotype-predicted intermediate CYP2D6 metabolizer.

Observation

A 49-year-old man was receiving chronic treatment for more than 6 years with paroxetine 60 mg/day for an indication of agoraphobia. The patient ingested fifty 20 mg tablets of paroxetine in a suicide attempt. The toxic plasma level, accompanied by delirium, persisted for approximately 1 month after the overdose. According to the genotype profile, the patient was evaluated as an intermediate metabolizer with reduced CYP2D6 enzyme activity.

Conclusion

As a consequence of the suicide attempt with overdose and the chronic paroxetine treatment that preceded it, phenoconversion to a poor metabolizer with very low CYP2D6 enzyme activity is suggested as contributing to an extremely long intoxication accompanied by delirium. Prolonged monitoring over a standard 24 h of both physical symptoms and drug plasma levels, together with a genetic profile assessment and phenoconversion consideration, is recommended after a single overdose in patients chronically treated with paroxetine.