bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Modification of Amorphous Silica with Ammonium Agents on the Physicochemical Properties and Hydrogenation Activity of Ir/SiO2 Catalysts
Authors: Kot Monika , Wojcieszak Robert , Janiszewska Ewa , Pietrowski Mariusz , Zieliński Michał
Published: February 2021

The modification of commercial silica with solutions of NH4F or NH4Cl salts, followed by thermal treatment, enabled generation of the acidic sites in SiO2 and changed its textural properties. The use of ammonium salts solution also caused the generation of additional porosity. Using NH4F solution caused significant decrease in the specific surface area and the increase in the average pore diameter. The number and strength of resulting acid sites depend on the nature of anion in the applied ammonium salt and the concentration of salt solution. It has been found that the sample treated with NH4F presented higher total acidity (TPD–NH3) and the amount as well as the strength of acid sites increased with the concentration of the used modifier. As modified amorphous SiO2 materials used as a support for iridium (1 wt %, Ir(acac)3) nanoparticles permitted to obtain highly active catalysts for toluene hydrogenation under atmospheric pressure. The highest activity (expressed as the apparent rate and TOF) was obtained for iridium catalysts supported on silica modified by NH4F with the highest acidity. The modification of silica with NH4F favors the generation of centers able to adsorb toluene, which results in higher activity of this catalyst.
Static and Dynamic Performances of Chopped Carbon-Fiber-Reinforced Mortar and Concrete Incorporated with Disparate Lengths
Authors: Li Yeou-Fong , Lee Kun-Fang , Ramanathan Gobinathan Kadagathur , Cheng Ta-Wui , Huang Chih-Hong , Tsai Ying-Kuan
Published: February 2021

The impact load, such as seismic and shock wave, sometimes causes severe damage to the reinforced concrete structures. This study utilized different lengths of chopped carbon fibers to develop a carbon-fiber-reinforced mortar (CFRM) and carbon-fiber-reinforced concrete (CFRC) with high impact and anti-shockwave resistance. The different lengths (6, 12, and 24 mm) of chopped carbon fibers were pneumatically dispersed and uniformly mixed into the cement with a 1% weight proportion. Then the CFRM and CFRC specimens were made for static and dynamic tests. The compressive and flexural strengths of the specimens were determined by using the standard ASTM C39/C 39M and ASTM C 293-02, respectively. Meanwhile, a free-fall impact test was done according to ACI 544.2R-89, which was used to test the impact resistances of the specimens under different impact energies. The CFRM and CFRC with a length of 6 mm exhibit maximum compressive strength. Both flexural and free-fall impact test results show that the 24 mm CFRM and CFRC enhances their maximum flexural strength and impact numbers more than the other lengths of CFRM, CFRC, and the benchmark specimens. After impact tests, the failure specimens were observed in a high-resolution optical microscope, to identify whether the failure mode is slippage or rupture of the carbon fiber. Finally, a blast wave explosion test was conducted to verify that the blast wave resistance of the 24 mm CFRC specimen was better than the 12 mm CFRC and benchmark specimens.
Investigating the Underlying Effect of Thermal Modification on Shrinkage Behavior of Bamboo Culm by Experimental and Numerical Methods
Authors: Ramful Raviduth , Sunthar Thefye P. M. , Zhu Wenliang , Pezzotti Giuseppe
Published: February 2021

This study probes into the root cause of split in thermally modified bamboo culm by investigating the underlying effect of thermal contraction with respect to its orthotropic nature by experimental and numerical methods while concurrently monitoring the chemical variation of its structure by Fourier transformed infrared spectroscopy (FTIR). In first part of this study, a non-linear increase in dimensional and weight changes of small clear bamboo specimens were observed with increasing temperature. The dimensional changes in the radial and tangential directions significantly exceeded that in the longitudinal direction. From FTIR results, shrinkage effect between 150 °C to 200 °C was associated with weight loss engendered by reduction in weakly bound water and increase in desorption of water content while alteration of its mechanical properties was attributed to changes in cellulose, hemicellulose, and lignin. From results of finite element method (FEM), the graded variation in thermal expansion coefficient, which showed the formation of a narrowed region of strain concentration corresponding to longitudinal crack propagation, was associated with the inducement of internal forces, namely tensile and compressive forces, at specific regions along the culm length. The results of this study can be useful to achieve optimized durability in modified bamboo for construction.
Epitaxial Growth and Characterization of 4H-SiC for Neutron Detection Applications
Authors: Meli Alessandro , Muoio Annamaria , Trotta Antonio , Meda Laura , Parisi Miriam , La Via Francesco
Published: February 2021

The purpose of this work is to study the 4H-SiC epitaxial layer properties for the fabrication of a device for neutron detection as an alternative material to diamond detectors used in this field. We have studied a high growth rate process to grow a thick epitaxial layer (250 µm) of 4H-SiC and, in order to estimate the quality of the epitaxial layer, an optical characterization was done through Photoluminescence (PL) spectroscopy for stacking fault defect evaluation. Micro Raman spectroscopy was used for simultaneous determination of both carrier lifetime and induced carriers in equilibrium. We have compared these results with other two samples with an epitaxial layer of 100 micron, obtained with two different growth rates, 60 and 90 µm/h, respectively. From Raman measurements it has been observed that both the growth rate and the grown epitaxial layer thickness have an effect on the measured carrier lifetime. A comparison between different kinds of stacking faults (SF) was done, evaluating the influence of these defects on the carrier lifetime as a function of the injection level and it was observed that only at a low injection is the effect on the carrier lifetime low.
Comparative Evaluation of Cu(acac)2 and {[Cu(μ-O,O′-NO3) (L-arg) (2,2′-bpy)]·NO3}n as Potential Precursors of Electroless Metallization of Laser-Activated Polymer Materials
Authors: Jagodziński Bartłomiej , Rytlewski Piotr , Moraczewski Krzysztof
Published: February 2021

This paper presents a comparative assessment of Cu(acac)2 and {[Cu(μ-O,O′-NO3) (L-arg)(2,2′-bpy)]·NO3}n as potential precursors for the electroless metallization of laser activated polymer materials. Coatings consisting of polyurethane resin, one of the two mentioned precursor compounds, and antimony oxide (Sb2O3), as a compound strongly absorbing infrared radiation, were applied on the polycarbonate substrate. The coatings were activated with infrared Nd: YAG laser radiation (λ = 1064 nm) and electroless metallized. It was found that after laser irradiation, a micro-rough surface structure of the coatings was formed, on which copper was present in various oxidation states, as well as in its metallic form. For selected parameters of laser irradiation, it was possible to deposit a copper layer on the coating containing Cu(acac)2 and Sb2O3, which is characterized by high adhesion strength. It was also found that the {[Cu(μ-O,O′-NO3) (L-arg)(2,2′-bpy)]·NO3}n complex was not an effective precursor for the electroless metallization of Nd:YAG laser activated coatings. An attempt was made to determine the influence of the precursor chemical structure on the obtained metallization effects.
Rare Occurrence of Microsatellite Instability in Gastrointestinal Stromal Tumors
Authors: Park Joonhong , Sul Hae Jung , Kim Jeong Goo
Published: February 2021

Background and Objectives: This study aimed to objectively determine microsatellite instability (MSI) status using a next-generation sequencing (NGS)-based MSI panel and to resolve the discrepancy regarding whether or not MSI is a rare phenomenon, irrespective of diverse genomic alterations in gastrointestinal stromal tumors (GISTs). Materials and Methods: Genomic DNA was subjected to MSI panel sequencing using an Ion AmpliSeq Microsatellite Instability Assay, as well as to cancer gene panel sequencing using an Oncomine Focus DNA Assay. Results: All of our GIST patients showed microsatellite-stable (MSS) status, which confirmed that MSI status did not affect the molecular pathogenesis of GIST. The KIT gene (79%, 38/48) was the most frequently mutated gene, followed by the PDGFRA (8%, 4/48), PIK3CA (8%, 4/48), and ERBB2 (4%, 2/48) mutations. KIT exon 11 mutant patients were more favorable in responding to imatinib than those with exon 9 mutant or wild-type GISTs, and compared to non-KIT exon 11 mutant GISTs (p = 0.041). The NGS-based MSI panel with MSICall confirmed a rare phenomenon of microsatellite instability in GISTs irrespective of diverse genomic alterations. Conclusion: Massively parallel sequencing can simultaneously provide the MSI status as well as the somatic mutation profile in a single test. This combined approach may help us to understand the molecular pathogenesis of GIST carcinogenesis and malignant progression.
Platelet Activity and Its Correlation with Inflammation and Cell Count Readings in Chronic Heart Failure Patients with Reduced Ejection Fraction
Authors: Mongirdienė Aušra , Laukaitienė Jolanta , Skipskis Vilius , Kuršvietienė Lolita , Liobikas Julius
Published: February 2021

Background and objectives: There has been an increasing interest in the role of inflammation in thrombosis complications in chronic heart failure (HF) patients. The incidence of thrombosis in HF has been shown to be the highest in patients classified as NYHA IV (New York Heart association). It is stated that inflammation is regulated by platelet-induced activation of blood leukocytes. We aimed to compare the platelet and cell count readings in chronic HF with reduced ejection fraction (HFrEF) patients according to NYHA functional class and to evaluate the correlation between those readings. Materials and methods: A total of 185 patients were examined. The results of heart echoscopy (TEE) testing; fibrinogen, N-terminal pro b-type natriuretic peptide (NT-proBNP), C reactive protein (CRP), and cortisol concentrations; complete blood counts; and a 6 min walking test were assessed and platelet aggregation was determined. Results: Mean platelet volume (MPV) increased with deterioration of a patient’s state (p < 0.005). Lymphocyte count and percentage were the lowest in the NYHA IV group (p < 0.005). Neutrophil and monocyte percentage and count were the highest (p < 0.045) in the NYHA IV group. Adenosine diphosphate (ADP)- and ADR-induced platelet aggregation was higher in the NYHA III group compared to NYHA II and I groups (p < 0.023). NYHA functional class correlated with mean platelet volume (MPV) (r = 0.311, p = 0.0001), lymphocyte count (r = −0.186, p = 0.026), monocyte count (p = 0.172, p = 0.041), and percentage (r = 0.212, p = 0.011). CRP concentration correlated with NT-proBNP (r = 0.203, p = 0.005). MPV correlated with fibrinogen concentration (r = 0.244, p = 0.004). Conclusions: (1) MPV could be considered as an additional reading reflecting a patient’s condition, however the use of MPV to identify patients at risk of hypercoagulable state should be evaluated in more extensive studies; (2) increased neutrophil and monocyte counts could indicate a higher inflammatory state in chronic HFrEF.
Selected Biochemical Blood Parameters and a Risk of Pressure Ulcers in Patients Receiving Treatment in Intensive Care Units
Authors: Bazaliński Dariusz , Midura Beata , Wójcik Anna , Więch Paweł
Published: February 2021

Background and Objectives: This study aimed to assess the level of selected biochemical parameters in venous blood and their potential effects on the development of pressure ulcers in patients treated in intensive care settings. Materials and Methods: Fifty patients hospitalised in an intensive care unit (ICU) were enrolled for the study. The methods used included controlled observation, literature review and medical record analysis. The observation protocol applied in the study consisted of two parts comprising the basic information, sociodemographic data, results of laboratory tests (CRP, PCT, albumin, protein and haemoglobin concentrations) as well as the Braden Scale for Predicting Pressure Ulcer Risk. Results: The subjects presented moderate to high risk of pressure ulcers, reflected by the mean score of 8.18 ± 1.3 points, with minimum and maximum scores of 6 and 12 points, respectively. Normal albumin level was identified in only five subjects (10.0%) while 45 subjects (90.0%) were found with results below the norm. A statistical relationship was observed between such variables as albumin concentration (p < 0.01) and total protein level (p = 0.007). The findings show a strong correlation between the score in the Braden Scale and the level of albumins (R = 0.55). Conclusions: In our study, lower concentrations of albumins and total proteins correspond to a greater risk of pressure ulcers.
Short-Term Effect of Orthodontic Treatment with Clear Aligners on Pain and sEMG Activity of Masticatory Muscles
Authors: Nota Alessandro , Caruso Silvia , Ehsani Shideh , Ferrazzano Gianmaria Fabrizio , Gatto Roberto , Tecco Simona
Published: February 2021

Background and objectives: The aim of this study is to evaluate mandibular elevator muscles activity and pain on palpation in the early stages of orthodontic treatment with clear aligners using surface electromyography (sEMG). Materials and methods: Surface electromyography (sEMG) activity and pain level on muscle palpation of masseter and anterior temporalis muscles were recorded in a sample of 16 adult subjects (aged 18–32 years; mean 22.5 +/− 3.5 SD) undergoing orthodontic treatment with clear aligners before the treatment (T0), after 1 month of treatment (two clear aligners) (T1), and after 3 months of treatment (T2) (six clear aligners). A chi-square test for nominal data, a Friedman test, and a Wilcoxon-signed rank test as post hoc analysis were applied. Results: No statistically significant differences in muscular pain were observed. At T1, the sEMG activity of masseter muscles at mandibular rest position showed a statistically significant reduction, but after 3 months (T2), the data appeared similar to T0 (p = 0.03 and p = 0.02). Conclusions: During the treatment with clear aligners, subjects could experience an initial reduction in the masseter basal activity after 1 month of treatment. This effect tends to decrease to baseline levels after 3 months of therapy.
Merits—A Journal of Virtues and Talents and Human Resources
Authors: Lin Shu-Kun
Published: February 2021

By way of introducing our new journal, Merits, allow me first to explain what the word “merits” means to me [...]
Untargeted and Targeted Metabolomic Profiling of Preterm Newborns with EarlyOnset Sepsis: A Case-Control Study
Authors: Mardegan Veronica , Giordano Giuseppe , Stocchero Matteo , Pirillo Paola , Poloniato Gabriele , Donadel Enrica , Salvadori Sabrina , Giaquinto Carlo , Priante Elena , Baraldi Eugenio
Published: February 2021

Sepsis is a major concern in neonatology, but there are no reliable biomarkers for its early diagnosis. The aim of the study was to compare the metabolic profiles of plasma and urine samples collected at birth from preterm neonates with and without earlyonset sepsis (EOS) to identify metabolic perturbations that might orient the search for new early biomarkers. All preterm newborns admitted to the neonatal intensive care unit were eligible for this proof-of-concept, prospective case-control study. Infants were enrolled as “cases” if they developed EOS, and as “controls”if they did not. Plasma samples collected at birth and urine samples collected within 24 h of birth underwent untargeted and targeted metabolomic analysis using mass spectrometry coupled with ultra-performance liquid chromatography. Univariate and multivariate statistical analyses were applied. Of 123 eligible newborns, 15 developed EOS. These 15 newborns matched controls for gestational age and weight. Metabolomic analysis revealed evident clustering of the cases versus controls, with the glutathione and tryptophan metabolic pathways markedly disrupted in the former. In conclusion, neonates with EOS had a metabolic profile at birth that clearly distinguished them from those without sepsis, and metabolites of glutathione and tryptophan pathways are promising as new biomarkers of neonatal sepsis.
The Efficacy of Pilates on Urinary Incontinence in Korean Women: A Metabolomics Approach
Authors: Kang Gyumin , Lee Haelim , Shin Malsoon , Kim Jaekwan , Lee Sungki , Park Youngja
Published: February 2021

Pilates has been known as exercise intervention that improves the function of pelvic floor muscle (PFM) associated with impacting urinary incontinence (UI). This study investigated the effect of Pilates on UI in Korean women by determining the change in functional movement of PFM (FMP) and metabolic profiles. UI group with Pilates (UIP, n = 13) participated in 8-weeks Oov Pilates program, and 8 subjects were assigned to Control and UI group with no Pilates (UINP), respectively. Before and after 8 weeks, plasma samples were collected from all participants, and ultrasonography was used to measure the functional change of PFM for calculating FMP ratio. Plasma samples were analyzed by mass spectrometry to identify the change of metabolic features. After 8-weeks intervention, FMP ratio was remarkably decreased in UIP (48.1% ↓, p < 0.001), but not in Control and UINP (p > 0.05). In metabolic features, L-Glutamine (m/z: 147.07 [M + H]+), L-Cystathionine (m/z: 240.09 [M + NH4]+), L-Arginine (m/z: 197.1 [M + Na]+), and L-1-Pyrroline-3-hydroxy-5-carboxylate (m/z: 147.07 [M + NH4]+) were significantly elevated solely in UIP (p < 0.001). Our study elucidated that Pilates can ameliorate the FMP and enhance the specific metabolic characteristics, which was potentially associated with invigorated PFM contractility to effectively control the bladder base and continence.
Phytochemicals of Conocarpus spp. as a Natural and Safe Source of Phenolic Compounds and Antioxidants
Authors: Afifi Hanan S. , Al Marzooqi Hassan M. , Tabbaa Mohammad J. , Arran Ahmed A.
Published: February 2021

Optimization of the extraction conditions of polyphenolic compounds for different parts of the Damas species, Conocarpus lancifolius and Conocarpus erectus, grown under UAE conditions was studied. The combination of ethanol concentration (50, 75, and 100%), temperature (45, 55, and 65 °C) and time (1, 2, and 3 h) was used by applying the Response Surface Methodology. The data showed that the extracts (n = 90) contained phenolic compounds, flavonoids, and tannins, and were free of alkaloids. Changing the extraction conditions had a significant effect on the detection of phytosterols, saponins, and glycosides and on the solubility of vanillic acid, p-coumaric acid, sinapic acid, t-ferulic acid, rutin hydrate, protocatechuic acid, quercetin, and flavone. The data reveal that the roots and leaves of C. erectus and the leaves and fruits of C.lancifolius are the most important plant parts from which to extract these compounds. This study draws attention to the unordinary use of Conocarpus spp. as a source of natural food additive.
Abdominothoracic Postural Tone Influences the Sensations Induced by Meal Ingestion
Authors: Livovsky Dan M. , Barber Claudia , Barba Elizabeth , Accarino Anna , Azpiroz Fernando
Published: February 2021

Postprandial objective abdominal distention is frequently associated with a subjective sensation of abdominal bloating, but the relation between both complaints is unknown. While the bloating sensation has a visceral origin, abdominal distention is a behavioral somatic response, involving contraction and descent of the diaphragm with protrusion of the anterior abdominal wall. Our aim was to determine whether abdominal distention influences digestive sensations. In 16 healthy women we investigated the effect of intentional abdominal distention on experimentally induced bloating sensation (by a meal overload). Participants were first taught to produce diaphragmatic contraction and visible abdominal distention. After a meal overload, sensations of bloating (0 to 10) and digestive well-being (−5 to + 5) were measured during 30-s. maneuvers alternating diaphragmatic contraction and diaphragmatic relaxation. Compared to diaphragmatic relaxation, diaphragmatic contraction was associated with diaphragmatic descent (by 21 + 3 mm; p < 0.001), objective abdominal distension (32 + 5 mm girth increase; p = 0.001), more intense sensation of bloating (7.3 + 0.4 vs. 8.0 + 0.4 score; p = 0.010) and lower digestive well-being (−0.9 + 0.5 vs. −1.9 + 0.5 score; p = 0.028). These results indicate that somatic postural tone underlying abdominal distention worsens the perception of visceral sensations (ClinicalTrials.gov ID: NCT04691882).
Narrative Review of n-3 Polyunsaturated Fatty Acid Supplementation upon Immune Functions, Resolution Molecules and Lipid Peroxidation
Authors: Zaloga Gary P.
Published: February 2021

Fish oil supplementation is commonplace in human nutrition and is being used in both enteral and parenteral formulations during the treatment of patients with a large variety of diseases and immune status. The biological effects of fish oil are believed to result from their content of n-3 polyunsaturated fatty acids (PUFA), particularly docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). These fatty acids are known to have numerous effects upon immune functions and are described as immunomodulatory. However, immunomodulatory is a nondescript term that encompasses immunostimulation and immunosuppression. The primary goal of this review is to better describe the immune effects of n-3 PUFA as they relate to immunostimulatory vs. immunosuppressive effects. One mechanism proposed for the immune effects of n-3 PUFA relates to the production of specialized pro-resolving mediators (SPMs). A second goal of this review is to evaluate the effects of n-3 PUFA supplementation upon production of SPMs. Although n-3 PUFA are stated to possess anti-oxidative properties, these molecules are highly oxidizable due to multiple double bonds and may increase oxidative stress. Thus, the third goal of this review is to evaluate the effects of n-3 PUFA upon lipid oxidation. We conclude, based upon current scientific evidence, that n-3 PUFA suppress inflammatory responses and most cellular immune responses such as chemotaxis, transmigration, antigen presentation, and lymphocyte functions and should be considered immunosuppressive. n-3 PUFA induced production of resolution molecules is inconsistent with many resolution molecules failing to respond to n-3 PUFA supplementation. n-3 PUFA supplementation is associated with increased lipid peroxidation in most studies. Vitamin E co-administration is unreliable for prevention of the lipid peroxidation. These effects should be considered when administering n-3 PUFA to patients that may be immunosuppressed or under high oxidative stress due to illness or other treatments.
Is Probiotic Supplementation Useful for the Management of Body Weight and Other Anthropometric Measures in Adults Affected by Overweight and Obesity with Metabolic Related Diseases? A Systematic Review and Meta-Analysis
Authors: Perna Simone , Ilyas Zahra , Giacosa Attilio , Gasparri Clara , Peroni Gabriella , Faliva Milena Anna , Rigon Chiara , Naso Maurizio , Riva Antonella , Petrangolini Giovanna , A. Redha Ali A. , Rondanelli Mariangela
Published: February 2021

The aim of this systematic review and meta-analysis is to assess the effectiveness of probiotics in inducing body weight loss in patients with overweight or obesity with related metabolic diseases. The research was carried out on PubMed and Scopus, focusing on studies reporting the effect on anthropometric measures (weight, body mass Index (BMI), waist circumference (WC), and hip circumference (HC) after administration of various probiotic strains compared to placebo. Twenty randomized controlled trials, that included 1411 patients, were considered. The meta-analyzed mean differences (MD) for random effects showed no significant decrease in body weight after probiotic supplementation (−0.26 kg [−075, 0.23], p = 0.30), while a significant BMI decrease was found (−0.73 kg/m2 [−1.31, −0.16], p = 0.01). For WC and HC, the meta-analyzed MD for random effects showed a significant decrease (WC: −0.71 cm [−1.24; −0.19], p = 0.008 and HC: −0.73 cm [−1.16; −0.30], p = 0.0008). The risk of bias was also evaluated considering a high risk and a low risk according to PRISMA criteria. In conclusion, the results of this meta-analysis highlight a positive trend of probiotics supplementation on the amelioration of anthropometric measures of overweight and obese patients with related metabolic diseases. However, further research is needed before recommending the use of probiotics as a therapeutic strategy for these patients. The focus of the future research should be to evaluate the efficacy of different probiotic strains, the quantities to be administered, and the duration of the intervention.
Murine Typhus Infection in Pregnancy: Case Series and Literature Review
Authors: Tanabe Melinda B. , Blanton Lucas S. , La Rosa Mauricio , Webb Camille M.
Published: February 2021

Murine typhus is a flea-borne disease of worldwide distribution with a recent reemergence in the United States of America. There are limited data about the presentation, treatment, and outcomes in the pregnant population. We report on two cases of murine typhus during pregnancy and review the literature to compile previously reported cases. A comprehensive search was performed via the PubMed database for published articles between 1990 and 2020. Seven articles met the criteria of symptomatic pregnant murine typhus infection. A total of 37 patients were identified. Patients frequently presented with a prolonged duration of fevers prior to presentation, headache, and elevated hepatic transaminases. The diagnosis was predominantly based on serology. Treatment varied. Overall, the pregnancy outcome was favorable. Murine typhus can mimic other pregnancy-related pathologies. More exclusive and large-scale studies are needed to learn more of murine typhus during pregnancy.
Exposure to Quaternary Ammonium Compounds Selects Resistance to Ciprofloxacin in Listeria monocytogenes
Authors: Guérin Alizée , Bridier Arnaud , Le Grandois Patricia , Sévellec Yann , Palma Federica , Félix Benjamin ,   LISTADAPT Study Group, Roussel Sophie , Soumet Christophe
Published: February 2021

In this contribution, the antimicrobial susceptibility toward 11 antibiotics and four biocides of a panel of 205 Listeria monocytogenes (Lm) strains isolated from different ecological niches (i.e., food, animals and natural environment) was evaluated. The impact of exposure to biocides on the antibiotic susceptibilities of Lm was also investigated. Lm strains isolated from food exhibited overall a lower susceptibility (higher minimal inhibitory concentrations, MIC) for ammonium quaternary compounds (QACs) and peracetic acid (PAC) than strains isolated from animals and natural environments. Conversely, the ecological origins of Lm strains did not significantly affect their susceptibilities towards antibiotics. Interestingly, repeated exposure to QACs recurrently led to a decrease in susceptibility toward ciprofloxacin (CIP), a fluoroquinolone antibiotic, largely used in human medicine. Moreover, these lower levels of susceptibility to CIP remained stable in most Lm strains even after subcultures without biocide selection pressure, suggesting an adaptation involving modifications at the genetic level. Results underlined the ability of Lm to adapt to biocides, especially QACs, and the potential link between this adaptation and the selection of resistance toward critical antibiotics such as ciprofloxacin. These data support a potential role of the extensive use of QACs from “farm to fork” in the selection of biocide and antibiotic resistance in pathogenic bacteria such as Lm.
Borrelia burgdorferi Surface Exposed GroEL Is a Multifunctional Protein
Authors: Cafiero Thomas , Toledo Alvaro
Published: February 2021

The spirochete, Borrelia burgdorferi, has a large number of membrane proteins involved in a complex life cycle, that includes a tick vector and a vertebrate host. Some of these proteins also serve different roles in infection and dissemination of the spirochete in the mammalian host. In this spirochete, a number of proteins have been associated with binding to plasminogen or components of the extracellular matrix, which is important for tissue colonization and dissemination. GroEL is a cytoplasmic chaperone protein that has previously been associated with the outer membrane of Borrelia. A His-tag purified B. burgdorferi GroEL was used to generate a polyclonal rabbit antibody showing that GroEL also localizes in the outer membrane and is surface exposed. GroEL binds plasminogen in a lysine dependent manner. GroEL may be part of the protein repertoire that Borrelia successfully uses to establish infection and disseminate in the host. Importantly, this chaperone is readily recognized by sera from experimentally infected mice and rabbits. In summary, GroEL is an immunogenic protein that in addition to its chaperon role it may contribute to pathogenesis of the spirochete by binding to plasminogen and components of the extra cellular matrix.
Pneumocystis Pneumonia: Immunity, Vaccines, and Treatments
Authors: Gingerich Aaron D. , Norris Karen A. , Mousa Jarrod J.
Published: February 2021

For individuals who are immunocompromised, the opportunistic fungal pathogen Pneumocystis jirovecii is capable of causing life-threatening pneumonia as the causative agent of Pneumocystis pneumonia (PCP). PCP remains an acquired immunodeficiency disease (AIDS)-defining illness in the era of antiretroviral therapy. In addition, a rise in non-human immunodeficiency virus (HIV)-associated PCP has been observed due to increased usage of immunosuppressive and immunomodulating therapies. With the persistence of HIV-related PCP cases and associated morbidity and mortality, as well as difficult to diagnose non-HIV-related PCP cases, an improvement over current treatment and prevention standards is warranted. Current therapeutic strategies have primarily focused on the administration of trimethoprim-sulfamethoxazole, which is effective at disease prevention. However, current treatments are inadequate for treatment of PCP and prevention of PCP-related death, as evidenced by consistently high mortality rates for those hospitalized with PCP. There are no vaccines in clinical trials for the prevention of PCP, and significant obstacles exist that have slowed development, including host range specificity, and the inability to culture Pneumocystis spp. in vitro. In this review, we overview the immune response to Pneumocystis spp., and discuss current progress on novel vaccines and therapies currently in the preclinical and clinical pipeline.
The Foliar Application of Rice Phyllosphere Bacteria induces Drought-Stress Tolerance in Oryza sativa (L.)
Authors: Devarajan Arun Kumar , Muthukrishanan Gomathy , Truu Jaak , Truu Marika , Ostonen Ivika , Kizhaeral S. Subramanian , Panneerselvam Periyasamy , Kuttalingam Gopalasubramanian Sabarinathan
Published: February 2021

This study assessed the potential of Bacillus endophyticus PB3, Bacillus altitudinis PB46, and Bacillus megaterium PB50 to induce drought tolerance in a susceptible rice cultivar. The leaves of the potted rice plants subjected to physical drought stress for 10 days during the flowering stage were inoculated with single-strain suspensions. Control pots of irrigated and drought-stressed plants were included in the experiment for comparison. In all treatments, the plant stress-related physiochemical and biochemical changes were examined and the expression of six stress-responsive genes in rice leaves was evaluated. The colonization potential on the surface of the rice leaves and stomata of the most successful strain in terms of induced tolerance was confirmed in the gnotobiotic experiment. The plants sprayed with B. megaterium PB50 showed an elevated stress tolerance based on their higher relative water content and increased contents of total sugars, proteins, proline, phenolics, potassium, calcium, abscisic acid, and indole acetic acid, as well as a high expression of stress-related genes (LEA, RAB16B, HSP70, SNAC1, and bZIP23). Moreover, this strain improved yield parameters compared to other treatments and also confirmed its leaf surface colonization. Overall, this study indicates that the foliar application of B. megaterium PB50 can induce tolerance to drought stress in rice.
Study of Sexual-Linked Genes (OGI and MeGI) on the Performance of Androecious Persimmons (Diospyros kaki Thunb.)
Authors: Wang Liyuan , Han Weijuan , Diao Songfeng , Suo Yujing , Li Huawei , Mai Yini , Wang Yiru , Sun Peng , Fu Jianmin
Published: February 2021

It is reported that the production of floral sexual phenotype in hexaploid monoecious persimmon (Diospyros kaki) is closely related to a pseudogene called OGI, and a short interspersed nuclear element (SINE)-like insertion (named Kali) in the OGI promoter leads to the gene silence. As a result, DNA methylation level of MeGI promoter determines the development of male or female flowers. However, the molecular mechanism in androecious D. kaki, which only bear male flowers, remains elusive. Here, real-time quantitative polymerase chain reaction (RT-qPCR), molecular cloning, and bisulfite PCR sequencing technique were carried out using 87 materials, including 56 androecious resources, 15 monoecious, and 16 gynoecious cultivars, to investigate the performance of OGI and MeGI on the specific androecious type of D. kaki in China. In conclusion, the Kali insertion was exactly located in the OGI promoter region, and the OGI gene and the Kali sequence were existing and conserved in androecious D. kaki. Meanwhile, we also demonstrated that the MeGI gene was widespread in our investigated samples. Ultimately, our result convincingly provided evidence that the low expression of OGI is probably ascribed to the presence of Kali displaying strong methylation in the OGI promoter, and low expression of MeGI, as well as high DNA methylation level, in the promoter was closely connected with the production of male flowers; this result was consistent with the monoecious persimmon model. Our findings provide predominant genetic aspects for investigation into androecious D. kaki, and future perfecting the sex-determining mechanisms in persimmon.
Evaluation of African Maize Cultivars for Resistance to Fall Armyworm Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) Larvae
Authors: Chiriboga Morales Xavier , Tamiru Amanuel , Sobhy Islam S. , Bruce Toby J. A. , Midega Charles A. O. , Khan Zeyaur
Published: February 2021

The fall armyworm (FAW) has recently invaded and become an important pest of maize in Africa causing yield losses reaching up to a third of maize annual production. The present study evaluated different aspects of resistance of six maize cultivars, cropped by farmers in Kenya, to FAW larvae feeding under laboratory and field conditions. We assessed the arrestment and feeding of FAW neonate larvae in no-choice and choice experiments, development of larvae-pupae, food assimilation under laboratory conditions and plant damage in a field experiment. We did not find complete resistance to FAW feeding in the evaluated maize cultivars, but we detected differences in acceptance and preference when FAW larvae were given a choice between certain cultivars. Moreover, the smallest pupal weight and the lowest growth index were found on ’SC Duma 43′ leaves, which suggests an effect of antibiosis of this maize hybrid against FAW larvae. In contrast, the highest growth index was recorded on ‘Rachar’ and the greatest pupal weight was found on ‘Nyamula’ and ‘Rachar’. The density of trichomes on the leaves of these maize cultivars seems not to be directly related to the preference of neonates for feeding. Plant damage scores were not statistically different between cultivars in the field neither under natural nor artificial infestation. However, plant damage scores in ‘Nyamula’ and ‘Jowi’ tended to be lower in the two last samplings of the season compared to the two initial samplings under artificial infestation. Our study provides insight into FAW larval preferences and performance on some African maize cultivars, showing that there are differences between cultivars in these variables; but high levels of resistance to larvae feeding were not found.
Optimization of a Wavy Microchannel Heat Sink with Grooves
Authors: Park Min-Cheol , Ma Sang-Bum , Kim Kwang-Yong
Published: February 2021

In this study, a wavy microchannel heat sink with grooves using water as the working fluid is proposed for application to cooling microprocessors. The geometry of the heat sink was optimized to improve heat transfer and pressure loss simultaneously. To achieve optimization goals, the average friction factor and thermal resistance were used as the objective functions. Three dimensionless parameters were selected as design variables: the distance between staggered grooves, groove width, and groove depth. A modified Latin hypercube sampling (LHS) method that combines the advantages of conventional LHS and a three-level full factorial method is also proposed. Response surface approximation was used to construct surrogate models, and Pareto-optimal solutions were obtained with a multi-objective genetic algorithm. The modified LHS was proven to have better performance than the conventional LHS and full factorial methods in the present optimization problem. A representative optimal design showed that both the thermal resistance and friction factor improved by 1.55% and 3.00%, compared to a reference design, respectively.
Ultrafiltration Method for Plasma Protein Binding Studies and Its Limitations
Authors: Toma Camelia-Maria , Imre Silvia , Vari Camil-Eugen , Muntean Daniela-Lucia , Tero-Vescan Amelia
Published: February 2021

Plasma protein binding plays a critical role in drug therapy, being a key part in the characterization of any compound. Among other methods, this process is largely studied by ultrafiltration based on its advantages. However, the method also has some limitations that could negatively influence the experimental results. The aim of this study was to underline key aspects regarding the limitations of the ultrafiltration method, and the potential ways to overcome them. The main limitations are given by the non-specific binding of the substances, the effect of the volume ratio obtained, and the need of a rigorous control of the experimental conditions, especially pH and temperature. This review presents a variety of methods that can hypothetically reduce the limitations, and concludes that ultrafiltration remains a reliable method for the study of protein binding. However, the methodology of the study should be carefully chosen.
Hyperspectral Image Spectral–Spatial Classification Method Based on Deep Adaptive Feature Fusion
Authors: Mu Caihong , Liu Yijin , Liu Yi
Published: February 2021

Convolutional neural networks (CNNs) have been widely used in hyperspectral image (HSI) classification. Many algorithms focus on the deep extraction of a single kind of feature to improve classification. There have been few studies on the deep extraction of two or more kinds of fusion features and the combination of spatial and spectral features for classification. The authors of this paper propose an HSI spectral–spatial classification method based on deep adaptive feature fusion (SSDF). This method first implements the deep adaptive fusion of two hyperspectral features, and then it performs spectral–spatial classification on the fused features. In SSDF, a U-shaped deep network model with the principal component features as the model input and the edge features as the model label is designed to adaptively fuse two kinds of different features. One comprises the edge features of the HSIs extracted by the guided filter, and the other comprises the principal component features obtained by dimensionality reduction of HSIs using principal component analysis. The fused new features are input into a multi-scale and multi-level feature extraction model for further extraction of deep features, which are then combined with the spectral features extracted by the long short-term memory (LSTM) model for classification. The experimental results on three datasets demonstrated that the performance of the proposed SSDF was superior to several state-of-the-art methods. Additionally, SSDF was found to be able to perform best as the number of training samples decreased sharply, and it could also obtain a high classification accuracy for categories with few samples.
Wetland Mapping Using HJ-1A/B Hyperspectral Images and an Adaptive Sparse Constrained Least Squares Linear Spectral Mixture Model
Authors: Na Xiaodong , Li Xingmei , Li Wenliang , Wu Changshan
Published: February 2021

In this study, we proposed an adaptive sparse constrained least squares linear spectral mixture model (SCLS-LSMM) to map wetlands in a sophisticated scene. It includes three procedures: (1) estimating the abundance based on sparse constrained least squares method with all endmembers in the spectral library, (2) selecting “active” endmember combinations for each pixel based on the estimated abundances and (3) estimating abundances based on the linear spectral unmixing algorithm only with the adaptively selected endmember combinations. The performances of the proposed SCLS-LSMM on wetland vegetation communities mapping were compared with the traditional full constrained least squares linear spectral mixture model (FCLS-LSMM) using HJ-1A/B hyperspectral images. The accuracy assessment results showed that the proposed SCLS-LSMM obtained a significantly better performance with a systematic error (SE) of –0.014 and a root-mean-square error (RMSE) of 0.087 for Reed marsh, and a SE of 0.004 and a RMSE of 0.059 for Weedy meadow, compared with the traditional FCLS-LSMM. The proposed methods improved the unmixing accuracies of wetlands’ vegetation communities and have the potential to understand the process of wetlands’ degradation under the impacts of climate changes and permafrost degradation.
Ionospheric Turbulence and the Equatorial Plasma Density Irregularities: Scaling Features and RODI
Authors: De Michelis Paola , Consolini Giuseppe , Tozzi Roberta , Pignalberi Alessio , Pezzopane Michael , Coco Igino , Giannattasio Fabio , Marcucci Maria Federica
Published: February 2021

In the framework of space weather, the understanding of the physical mechanisms responsible for the generation of ionospheric irregularities is particularly relevant for their effects on global positioning and communication systems. Ionospheric equatorial plasma bubbles are one of the possible irregularities. In this work, using data from the ESA Swarm mission, we investigate the scaling features of electron density fluctuations characterizing equatorial plasma bubbles. Results strongly support a turbulence character of these structures and suggest the existence of a clear link between the observed scaling properties and the value of the Rate Of change of electron Density Index (RODI). This link is discussed, and RODI is proposed as a reliable proxy for the identification of plasma bubbles.
Structural Characteristics of Moho Surface Based on Time Series Function of Natural Earthquakes
Authors: Li Xuelei , Jia Zhuo , Du Nanqiao , Xu Yi , Zhang Gongbo
Published: February 2021

Remote sensing is a non-contact, long-distance detection technology. The reflection characteristics of a seismic wave can be used to detect remote and non-contact targets. Based on the reflection characteristics of a seismic wave, the underground structure in Tengchong Volcanic Area is explored. In order to further study the deep structure and magmatic activity of the crust in the volcanic area, we carried out a one-year mobile seismic observation. In this paper, nine broadband seismic stations were set up in the Tengchong Volcanic Area, and 3350 receiver function waveforms were collected. The crustal thickness, average wave velocity ratio, and Poisson’s ratio below these stations were calculated by the receiver function method, and the velocity structure near the Moho below these stations was evaluated. Combined with topographic data from SRTM3 (Shuttle Radar Topography Mission 3), this study reveals the dynamic relationship among crustal structure, crustal magmatism, and regional tectonic movement. Mantle upwelling plays an important role on the Moho uplift in the northern Tengchong Volcanic Area, and there are interconnected intracrustal magma chambers in the upper platform. The evaluation results of the Moho transition zone also indicate that the Dayingjiang fault is closely related to the tectonic activity of the Tengchong Volcanic fault.
Accelerometry-Enhanced Magnetic Sensor for Intra-Oral Continuous Jaw Motion Tracking
Authors: Jucevičius Mantas , Ožiūnas Rimantas , Mažeika Mindaugas , Marozas Vaidotas , Jegelevičius Darius
Published: February 2021

Currently available jaw motion tracking methods require large accessories mounted on a patient and are utilized in controlled environments, for short-time examinations only. In some cases, especially in the evaluation of bruxism, a non-restrictive, 24-h jaw tracking method is needed. Bruxism oriented, electromyography (EMG)-based devices and sensor-enhanced occlusal splints are able to continuously detect masticatory activity but are uninformative in regards to movement trajectories and kinematics. This study explores a possibility to use a permanent magnet and a 3-axial magnetometer to track the mandible’s spatial position in relation to the maxilla. An algorithm for determining the sensor’s coordinates from magnetic field values was developed, and it was verified via analytical and finite element modeling and by using a 3D positioning system. Coordinates of the cubic test trajectory (a = 10 mm) were determined with root-mean-square error (RMSE) of 0.328±0.005 mm. Possibility for teeth impact detection by accelerometry was verified. Test on a 6 degrees-of-freedom (DOF), hexapod-based jaw motion simulator moving at natural speed confirmed the system’s ability to simultaneously detect jaw position and the impacts of teeth. Small size of MEMS sensors is suitable for a wearable intra-oral system that could allow visualization of continuous jaw movement in 3D models and could enable new research on parafunctional jaw activities.