bluuebunny/crossref_metadata_2025 huggingface dataset:
Aspiration Thrombectomy in Patients with Acute Myocardial Infarction—5-Year Analysis Based on a Large National Registry (ORPKI)
Authors: Januszek Rafał , Siudak Zbigniew , Malinowski Krzysztof P. , Wojdyła Roman , Mika Piotr , Wańha Wojciech , Kameczura Tomasz , Surdacki Andrzej , Wojakowski Wojciech , Legutko Jacek , Bartuś Stanisław
Published: November 2020

Blood flow restoration after primary percutaneous coronary intervention (pPCI) in patients with acute myocardial infarction (AMI) may not always be achieved and could be complicated by the no-reflow phenomenon (NRP). The aim of the current study was to assess the frequency of thrombus aspirations (TAs) and NRPs in patients with AMI and treated with pPCI based on the data collected during a 5-year period in the national ORPKI registry, as well as the frequency of periprocedural strokes and predictors of TA and NRP. This retrospective analysis was performed on prospectively collected data gathered in the Polish National Registry of Percutaneous Coronary Interventions (ORPKI), which covered the period between January 2014 and December 2018, and included 200,991 patients treated due to AMI out of 535,857 patients treated using PCI. Among them, 16,777 patients underwent TA. TA was mainly used in the STEMI subgroup of 14,207 patients (84.8%). The frequency of NRP among AMI patients in the thrombectomy group was 2.75% and in the non-thrombectomy group 0.82%. Predictors of TA and NRP were also assessed using multivariate analysis. The percentage of patients treated with pPCI and with PCI alone increased significantly in all of the three selected groups of patients from 88.7% to 94.3% in the AMI group (p < 0.001), from 82.3% to 90.3% in the STEMI subgroup (p < 0.001), and from 96.3% to 98.2% in the NSTEMI subgroup (p < 0.001) during the analysed period. NRP occurred more often in the thrombectomy group for the NSTEMI (0.58% vs. 3.07%, p < 0.05) and STEMI (1.06% vs. 2.69%, p < 0.05) subgroups. Periprocedural stroke occurred more often in the thrombectomy group in comparison to the non-thrombectomy group with AMI (0.03% vs. 0.01%, p < 0.05) and the NSTEMI (0.16% vs. 0.02%, p < 0.05). In conclusion, the frequency of TA has been experiencing a steady decline in recent years, regardless of AMI type, among patients treated with pPCI.
Continuous Non-Invasive Arterial Pressure Monitoring (ClearSight System) and Ankle Blood Pressure Measurements as Alternatives to Conventional Arm Blood Pressure
Authors: Lee Seohee , Chung Jaeyeon , Bae Jinyoung , Cho Youn Joung , Nam Karam , Jeon Yunseok
Published: November 2020

Measuring blood pressure (BP) via a pneumatic cuff placed around the arm has long been the standard method. However, in clinical situations where BP monitoring at the arm is difficult, the ankle is frequently used instead. We compared continuous non-invasive blood pressure (CNBP) measurements obtained at the finger, ankle BP and arm BP in patients undergoing breast cancer surgery. Arm BP, ankle BP (both obtained with a conventional pneumatic cuff) and CNBP measurements were obtained every 2.5 min during surgery. Correlation and Bland–Altman analyses were performed and differences among measurements were analyzed using a linear mixed model. A total of 245 sets of BP measurements were obtained from 10 patients. All systolic blood pressure (SBP), diastolic blood pressure (DBP) and mean blood pressure (MBP) measurements of ankle BP and CNBP were positively correlated with the arm BP measurements (Spearman rho 0.688–0.836, p < 0.001 for each correlation). The difference between CNBP and arm SBP was significantly smaller (least squares mean (95% confidence interval): −6.03 (−11.40, −0.67)) compared to that between ankle and arm SBP (least squares mean (95% CI): −15.32 (−20.69, −9.96), p = 0.019). However, this significant difference was not observed in DBP and MBP (−1.23 vs. 1.75, p = 0.190 and −3.85 vs. −2.63, p = 0.604, respectively). Ankle SBP measurements showed larger differences from arm SBP measurements than did CNBP SBP measurements in patients undergoing breast cancer surgery. CNBP could serve as a useful alternative to ankle BP when standard arm BP measurements cannot be obtained.
Differentiated Thyroid Cancer in Children in the Last 20 Years: A Regional Study in Romania
Authors: Ștefan Andreea-Ioana , Piciu Andra , Căinap Simona Sorana , Gabora Katalin , Piciu Doina
Published: November 2020

Pediatric thyroid carcinoma is a current area of interest, because there are few studies in this field; the current classification and treatment guidelines are extrapolated from adults, sometimes leading to overly aggressive treatments or incomplete treatment of the disease. The purpose of this retrospective study is to analyze the presentation, diagnosis, treatment, complications, and outcome of children diagnosed with thyroid cancer in the last two decades (2000–2018) at the Oncological Institute “Prof. Dr. Ion Chiricuță” Cluj-Napoca (IOCN) Romania, a tertiary center in a region with environmental influences from both the nuclear fallout of the Chernobyl accident and from iodine deficiency. The results were compared with those obtained in a previous study carried out in the same institution between 1991–2010, and with those obtained in a similar study carried out in Netherlands between 1970–2013, a cohort of children not exposed to the post-Chernobyl fallout. We statistically analyzed 62 patients. Papillary form was present in the majority of cases, and we highlighted the occurrence of thyroid microcarcinoma in children. A total of 85.4% of patients received radioiodine, the total activity being significantly lower compared with the data from literature. In our study, the prognosis of the disease was excellent, with 100% overall survival.
First Wave of COVID-19 in French Patients with Cystic Fibrosis
Authors: Corvol Harriet , de Miranda Sandra , Lemonnier Lydie , Kemgang Astrid , Reynaud Gaubert Martine , Chiron Raphael , Dalphin Marie-Laure , Durieu Isabelle , Dubus Jean-Christophe , Houdouin Véronique , Prevotat Anne , Ramel Sophie , Revillion Marine , Weiss Laurence , Guillot Loic , Boelle Pierre-Yves , Burgel Pierre-Régis
Published: November 2020

Viral infections are known to lead to serious respiratory complications in cystic fibrosis (CF) patients. Hypothesizing that CF patients were a population at high risk for severe respiratory complications from SARS-CoV-2 infection, we conducted a national study to describe the clinical expression of COVID-19 in French CF patients. This prospective observational study involves all 47 French CF centers caring for approximately 7500 CF patients. Between March 1st and June 30th 2020, 31 patients were diagnosed with COVID-19: 19 had positive SARS-CoV-2 RT-PCR in nasopharyngeal swabs; 1 had negative RT-PCR but typical COVID-19 signs on a CT scan; and 11 had positive SARS-CoV-2 serology. Fifteen were males, median (range) age was 31 (9–60) years, and 12 patients were living with a lung transplant. The majority of the patients had CF-related diabetes (n = 19, 61.3%), and a mild lung disease (n = 19, 65%, with percent-predicted forced expiratory volume in 1 s (ppFEV1) > 70). Three (10%) patients remained asymptomatic. For the 28 (90%) patients who displayed symptoms, most common symptoms at admission were fever (n = 22, 78.6%), fatigue (n = 14, 50%), and increased cough (n = 14, 50%). Nineteen were hospitalized (including 11 out of the 12 post-lung transplant patients), seven required oxygen therapy, and four (3 post-lung transplant patients) were admitted to an Intensive Care Unit (ICU). Ten developed complications (including acute respiratory distress syndrome in two post-lung transplant patients), but all recovered and were discharged home without noticeable short-term sequelae. Overall, French CF patients were rarely diagnosed with COVID-19. Further research should establish whether they were not infected or remained asymptomatic upon infection. In diagnosed cases, the short-term evolution was favorable with rare acute respiratory distress syndrome and no death. Post-lung transplant patients had more severe outcomes and should be monitored more closely.
Direct Numerical Simulations of the Pulsating Flow over a Plane Wall
Authors: Vittori Giovanna , Blondeaux Paolo , Mazzuoli Marco
Published: November 2020

The results of direct numerical simulations of the flow generated in a plane duct by a pressure gradient which is the sum of two terms are described. The first term of the pressure gradient is constant in space but it oscillates in time whereas the second term is constant both in space and in time. Therefore, a pulsating flow is generated, similar to that generated at the bottom of a monochromatic propagating surface wave when nonlinear effects are taken into account. The simulations are carried out for values of the parameters similar to those considered in previous investigations. It is shown that even a small constant pressure gradient influences the flow regime in the bottom boundary layer. In particular, turbulence strength is damped when the steady velocity component has the direction opposite to the oscillating velocity component whereas turbulence strength increases when the steady and oscillating components point in the same direction. Even though the flow is not exactly equal to that generated at the bottom of sea waves, where second order effects in the wave steepness induce a steady streaming in the direction of wave propagation, our results provide information on the interaction of the steady streaming with the oscillatory flow and are also relevant for investigating the dynamics of sediment close to the sea bottom. Indeed, since the turbulent eddies tend to pick-up the sediment from the bottom, it can be inferred that the triggering of turbulence enhances sediment transport towards the shore.
Development of Entering Order and Work-Volume Assignment Algorithms for the Management of Piping Components in Offshore Structure Construction
Authors: Park Jung Goo , Kim Ho Jung , Woo Jong Hun
Published: November 2020

In the early 2010s, with rising oil prices and increasing purchase orders for offshore structures for deep-sea resource development, the shipyards that took these orders suffered unexpected losses. Unlike the construction of commercial carrier vessels, the construction of offshore structures necessary to develop deep-sea resources is difficult to manage due to the complexity of the outfitting process of the topside structure, which is a plant for gas and oil production and treatment. Piping components in particular, which comprise most of the design items, are difficult to manage because they involve 2 to 3 times the man-hours and up to 10 times the quantity of items compared to commercial carrier vessels. Due to not only high man-hours and quantity but also large fluctuations caused by design changes and long procurement lead times, process delays that result in delayed compensation frequently occurred. In response, Samsung Heavy Industries developed an integrated management system for piping components. This study describes the entering order optimization algorithm and work-volume assignment optimization algorithm, which are the core algorithms of this system. The entering order optimization algorithm determines the optimal installation order considering the procurement status of the piping components and the installation readiness status of the installation work site, through which it determines the entering order of the piping components. The algorithm seeks to accelerate the completion rate of installation of the piping components. Next, to minimize delivery delays of sub-contractors to the shipyard, this study developed a work-volume assignment optimization algorithm that can equalize the load on multiple sub-contractors considering the raw material readiness status and the production capacity of the sub-contractors, in terms of materials that must be ordered from external sub-contractors among the piping components whose entering order was determined. Finally, applying the algorithm developed using actual shipyard data resulted in an accelerated completion rate of installation and improved balance of load in terms of volume assigned to the sub-contractors.
Preterm Infants Harbour a Rapidly Changing Mycobiota That Includes Candida Pathobionts
Authors: A. James Stephen , Phillips Sarah , Telatin Andrea , Baker David , Ansorge Rebecca , Clarke Paul , J. Hall Lindsay , R. Carding Simon
Published: November 2020

Fungi and the mycobiome are a fundamental part of the human microbiome that contributes to human health and development. Despite this, relatively little is known about the mycobiome of the preterm infant gut. Here, we have characterised faecal fungal communities present in 11 premature infants born with differing degrees of prematurity and mapped how the mycobiome develops during early infancy. Using an ITS1 sequencing-based approach, the preterm infant gut mycobiome was found to be often dominated by a single species, typically a yeast. Candida was the most abundant genus, with the pathobionts C.albicans and C.parapsilosis highly prevalent and persistent in these infants. Gestational maturity at birth affected the distribution and abundance of these Candida, with hospital-associated C.parapsilosis more prevalent and abundant in infants born at less than 31 weeks. Fungal diversity was lowest at 6 months, but increased with age and change of diet, with food-associated Saccharomycescerevisiae most abundant in infants post weaning. This study provides a first insight into the fungal communities present within the preterm infant gut, identifying distinctive features including the prominence of pathobiont species, and the influence age and environmental factors play in shaping the development of the mycobiome.
A Multidisciplinary Approach to Fungal Infections: One-Year Experiences of a Center of Expertise in Mycology
Authors: Janssen Nico A. F. , Brüggemann Roger J. M. , Reijers Monique H. , Henriet Stefanie S. V. , ten Oever Jaap , de Mast Quirijn , Berk Yvonne , de Kort Elizabeth A. , Kullberg Bart Jan , Netea Mihai G. , Buil Jochem B. , Rahamat-Langendoen Janette C. , Bury Didi , Muilwijk Eline W. , Meis Jacques F. , Verweij Paul E. , van de Veerdonk Frank L.
Published: November 2020

Invasive fungal diseases (IFDs) often represent complicated infections in complex patient populations. The Center of Expertise in Mycology Radboudumc/CWZ (EMRC) organizes a biweekly multidisciplinary mycology meeting to discuss patients with severe fungal infections and to provide comprehensive advice regarding diagnosis and treatment. Here, we describe the patient population discussed at these meetings during a one-year period with regards to their past medical history, diagnosis, microbiological and other diagnostic test results and antifungal therapy. The majority of patients discussed were adults (83.1%), 62.5% of whom suffered from pulmonary infections or signs/symptoms, 10.9% from otorhinolaryngeal infections and/or oesophagitis, 9.4% from systemic infections and 9.4% from central nervous system infections. Among children, 53.8% had pulmonary infections or signs/symptoms, 23.1% systemic fungal infections and 23.1% other, miscellaneous fungal infections. 52.5% of adult patients with pulmonary infections/symptoms fulfilled diagnostic criteria for chronic pulmonary aspergillosis (CPA). Culture or polymerase chain reaction (PCR) demonstrated fungal pathogens in 81.8% of patients, most commonly Aspergillus. A multidisciplinary mycology meeting can be a useful addition to the care for patients with (I)FDs and can potentially aid in identifying healthcare and research needs regarding the field of fungal infections. The majority of patients discussed at the multidisciplinary meetings suffered from pulmonary infections, predominantly CPA.
Candidemia in Children with Malignancies: Report from the Infection Working Group of the Hellenic Society of Pediatric Hematology-Oncology
Authors: Vasileiou Eleni , Paisiou Anna , Tsipou Charoula , Pourtsidis Apostolos , Galani Vasiliki , Katzilakis Nikolaos , Antoniadi Kondilia , Papakonstantinou Eugenia , Ioannidou Elda , Stiakaki Efthichia , Baka Margarita , Kattamis Antonios , Kitra Vasiliki , Tragiannidis Athanasios
Published: November 2020

Candidemia is an important cause of morbidity and mortality especially in immunocompromised and hospitalized patients. We retrospectively collected data of candidemia cases that occurred in the seven Hematology-Oncology Departments/Units of Greece and the Stem Cell Transplant Unit between 2015 and 2019. In total, 19 episodes of candidemia in 19 patients were recorded. The majority of the patients (78.9%) had at least one risk factor for candidemia. The most frequent risk factors associated with candidemia observed in our patients were prolonged duration of hospitalization (30 days, range 1–141), presence of a central venous catheter at diagnosis of candidemia (73.7%) and antibiotics use during the last two weeks (84.2%). Candida parapsilosis was the most common species isolated accounting for 42.1%, followed by C. albicans (26.3%) and C. famata (15.8%). Nearly all of the patients (84.2%) received antifungal monotherapy with liposomal amphotericin B or echinocandins. The central venous catheter was removed in 78.6% of patients and the median time between the first positive blood culture and catheter removal was 3 days (range 1–9). Mortality at 28 days was 26.3%. In conclusion, a predominance of non-albicans species was observed in our study in conformity with the global trend.
Local and Global Existence of Solution for Love Type Waves with Past History
Authors: Biomy Mohamed , Zennir Khaled , Himadan Ahmed
Published: November 2020

In this paper, we consider an initial boundary value problem for nonlinear Love equation with infinite memory. By combining the linearization method, the Faedo–Galerkin method, and the weak compactness method, the local existence and uniqueness of weak solution is proved. Using the potential well method, it is shown that the solution for a class of Love-equation exists globally under some conditions on the initial datum and kernel function.
The Natural History of Retinal Vascular Changes from Infancy to Adulthood in Wyburn-Mason Syndrome
Authors: Horkovicova Kristina , Popov Ivajlo , Tomcikova Dana , Popova Veronika , Krasnik Vladimir
Published: November 2020

Wyburn-Mason syndrome is a rare, non-hereditary congenital neurocutaneous disorder leading to arteriovenous malformations. Malformations are characterized by an artery that is directly connected to veins without a capillary system and forms a fragile mass of abnormal vessels. It can be found in the midbrain, in the eyes, orbit, and rarely in cutaneous nevi. Neurological and ocular symptoms are the most common. Ocular signs and symptoms include abnormally dilatated vessels of conjunctiva, nystagmus, strabismus, vitreous hemorrhage, vein occlusions, retinal detachment, etc. Neurological symptoms may include headaches, paralysis, epistaxis, hydrocephalus, and hemiparesis. Imaging modalities such as MRI/CT angiography, optical coherence angiography, and fluorescein angiography are the most important for the identification of arteriovenous malformations. In our case report, we present an eight-month-old girl with an incidental finding of retinal angiomatosis on the left eye and was subsequently diagnosed with Wyburn-Mason syndrome. We compare the findings from the first visit to her clinical findings 20 years later.
Histological Aspects and Quantitative Assessment of Ki67 as Prognostic Factors in Breast Cancer Patients: Result from a Single-Center, Cross Sectional Study
Authors: Niță Irina , Nițipir Cornelia , Toma Ștefania Andreea , Limbău Alexandra Maria , Pîrvu Edvina , Bădărău Ioana Anca , Suciu Ioana , Suciu George , Manolescu Loredana Sabina Cornelia
Published: November 2020

Background and objectives: Our aim is to explore the relationship between the levels of protein encoded by Ki67 and the histopathological aspects regarding the overall survival and progression-free survival in a single university center. A secondary objective was to examine other factors that can influence these endpoints. New approaches to the prognostic assessment of breast cancer have come from molecular profiling studies. Ki67 is a nuclear protein associated with cell proliferation. Together with the histological type and tumor grade, it is used to appreciate the aggressiveness of the breast tumors. Materials and Methods: We conducted a retrospective single-institution study, at Elias University Emergency Hospital, Bucharest, Romania, in which we enrolled women with stage I to III breast cancer. The protocol was amended to include the immunohistochemistry determination of Ki67 and the histological aspects. The methodology consisted in using a Kaplan–Meier analysis for the entire sample and restricted mean survival time up to 36 months. Results: Both lower Ki67 and low tumor grade are associated with better prognosis in terms of overall survival (OS) and progression-free survival (PFS) for our patients’ cohort. In our group, the histological type did not impact the time to progression or survival. Conclusions: Both overall survival and progression-free survival may be influenced by the higher value of Ki67 and less differentiated tumors. Further studies are needed in order to establish if the histologic type may impact breast cancer prognostic, probably together with other histologic and molecular markers.
Effect of Decompression on Jaw Cystic Lesions Based on Three-Dimensional Volumetric Analysis
Authors: Kwon Yeh-Jin , Ko Kyeong-Soo , So Byung-Kyu , Kim Dong-Hyuck , Jang Hyon-Seok , Kim Soo-Ho , Lee Eui-Seok , Lim Ho-Kyung
Published: November 2020

Background and objectives: This study aimed to evaluate the effectiveness of decompression on jaw cysts according to various parameters by volumetric analysis using three-dimensional computed tomography. Materials and methods: Fifty patients who underwent surgical decompression of the jaw cystic lesion were selected, and their preoperative and postoperative computed tomography results between 3 and 27 months were collected. Volumetric analysis was performed to evaluate any differences in the rate of volumetric change according to the sex, age, initial volume of the lesion, duration, location of the lesion, tooth extraction, expansion of the cortical layer, and pathological diagnosis. Multiple linear regression and generalised linear mixed models were used for statistical analyses. Results: The mean reduction rate among all patients was 54.68%. Multiple linear regression analysis revealed that higher reduction rates were associated with a long decompression period, young patient age, and location of the cyst in the posterior maxilla. Generalised linear mixed models revealed that higher reduction rates were associated with a long decompression period and young patient age. Conclusions: Decompression was an effective treatment for reducing the cyst size in all patients. Its effectiveness increased with a long treatment duration, young patient age, and cyst location in the posterior maxilla three-dimensionally.
Does Thyroidectomy Impact Quality of Life: Retrospective Case–Control Study of Post-Thyroidectomy Patients and Matched Individuals from the General Population
Authors: Yu Hyeong Won , An Ah Reum , Kang Hye In , Suh Yong Joon , Kwon Hyungju , Kim Su-jin , Chai Young Jun , Choi June Young , Choi Hochun , Lee Kyu Eun , Cho Belong
Published: November 2020

Background and objectives: The study assesses quality of life (QoL) in patients who underwent thyroidectomy compared to the general population. Materials and Methods: QoL data from post-thyroidectomy patients and individuals with no subjective health concerns, who had attended a routine health screening visit, were evaluated. QoL was assessed using the modified version of Korean Short Form 12 questionnaire (SF-12). Patients and controls were matched using the propensity score approach and a ratio of 1:4. Results: Data from a total of 105 patients and 420 controls were analyzed. For five SF-12 items, lower QoL was found in patients (p < 0.05). Multivariate analysis revealed that a follow-up duration of <1-year, female sex, and an age of >50 years were independent risk factors. No significant difference was found between controls and patients who were >1-year post-surgery. Conclusions: For specific SF-12 items, QoL was lower in post-thyroidectomy patients than in controls. No intergroup difference in QoL was found >1-year post-surgery.
AlMg6 to Titanium and AlMg6 to Stainless Steel Weld Interface Properties after Explosive Welding
Authors: Malakhov Andrey Y. , Saikov Ivan V. , Denisov Igor V. , Niyezbekov Nemat N.
Published: November 2020

This paper studies the weld interface microstructure and mechanical properties of AlMg6-stainless steel and AlMg6-titanium bimetals produced using explosive welding. The microhardness (HV), tear strength, and microstructure of the weld seams were evaluated. The interface of the weld zones had a flat profile. No structural disturbances or heterogeneity in the AlMg6-titanium weld interface were observed. On the other hand, the bimetal AlMg6-stainless steel had extensive zones of cast inclusions in the 10–30 µm range. SEM/energy-dispersive X-ray spectroscopy (EDS) analysis showed the presence of a hard and brittle intermetallic compound of Al and FeAl3 (with 770–800 HV). The microhardness of the AlMg6-titanium bimetal grew higher closer to the weld interface and reached 207 HV (for AlMg6) and 340 HV (for titanium). Both bimetals had average tear strength below 100 MPa. However, the tear strength of some specimens reached 186 and 154 MPa for AlMg6-titanium and AlMg6-stainless steel, respectively. It is also worth mentioning that heat treatment at 200 °C for one hour led to a uniform distribution of tear strength along the entire length of the bimetals. The study shows that one of the possible solutions to the problem of the formation of the brittle intermetallic compounds would be the use of intermediate layers of refractory metals.
Waste Slag from Heating Plants as a Partial Replacement for Cement in Mortar and Concrete Production. Part I—Physical–Chemical and Physical–Mechanical Characterization of Slag
Authors: Nedeljković Andrijana , Stojmenović Marija , Gulicovski Jelena , Ristić Nenad , Milićević Sonja , Krstić Jugoslav , Kragović Milan
Published: November 2020

Numerous factors influence the complexity of environmental and waste management problems, and the most significant goal is the reuse of materials that have completed their “life cycle” and the reduction in the use of new resources. In order to reduce impact of waste slag on the environment, in the present study, waste slag, generated in heating plants after lignite combustion, was characterized in detail and tested for application as a replacement for cement in mortar or concrete production. For physical–chemical characterization of slag, different experimental and instrumental techniques were used such as chemical composition and determination of the content of heavy metals, investigation of morphological and textural properties, thermal analysis, X-ray, and infrared spectroscopy. Physical–mechanical characterization of slag was also performed and included determination of activity index, water requirement, setting time and soundness. A leaching test was also performed. Presented results show that waste slag may be used in mortar and concrete production as a partial cement replacement, but after additional combustion at 650 °C and partial replacement of slag with silica fume in the minimal amount of 12%. The maximal obtained cement replacement was 20% (17.8% slag and 2.2% of silica fume).
Correction: Tallman, D.A., et al. Dietary Patterns and Health Outcomes among African American Maintenance Hemodialysis Patients. Nutrients 2020, 12(3), 797
Authors: Tallman Dina A. , Latifi Eno , Kaur Deepinder , Sulaheen Ayesha , Ikizler T. Alp , Chinna Karuthan , Daud Zulfitri Azuan Mat , Karupaiah Tilakavati , Khosla Pramod
Published: November 2020

The authors wish to make the following correction to this paper [...]
Depressive Symptoms in Middle-Aged and Elderly Women Are Associated with a Low Intake of Vitamin B6: A Cross-Sectional Study
Authors: Odai Tamami , Terauchi Masakazu , Suzuki Risa , Kato Kiyoko , Hirose Asuka , Miyasaka Naoyuki
Published: November 2020

This study investigated the nutritional factors that are associated with anxiety and depressive symptoms in Japanese middle-aged and elderly women. We conducted a cross-sectional study with 289 study participants aged ≥40 years (mean age = 52.0 ± 6.9 years). Their dietary habits, menopausal status and symptoms, and varied background factors, such as body composition, lifestyle factors, and cardiovascular parameters, were assessed. Their anxiety and depressive symptoms were evaluated using the Hospital Anxiety and Depression Scale (HADS), where scores of 0–7 points, 8–10 points, and 11–21 points on either the anxiety or depression subscales were categorized as mild, moderate, and severe, respectively. The dietary consumption of nutrients was assessed using a brief self-administered diet history questionnaire. The relationships between the moderate-to-severe anxiety/depressive symptoms and the dietary intake of 43 major nutrients were investigated using multivariate logistic regression analyses. After adjusting for age, menopausal status, and the background factors that were significantly related to depressive symptoms, moderate and severe depression was significantly inversely associated with only vitamin B6 (adjusted odds ratio per 10 μg/MJ in vitamin B6 intake = 0.89, 95% confidence interval = 0.80–0.99). A higher intake of vitamin B6 could help relieve depressive symptoms for this population.
Effects of a Fermented Dairy Drink Containing Lacticaseibacillus paracasei subsp. paracasei CNCM I-1518 (Lactobacillus casei CNCM I-1518) and the Standard Yogurt Cultures on the Incidence, Duration, and Severity of Common Infectious Diseases: A Systematic Review and Meta-Analysis of Randomized Controlled Trials
Authors: Poon Theresa , Juana Justine , Noori Daniel , Jeansen Stephanie , Pierucci-Lagha Amira , Musa-Veloso Kathy
Published: November 2020

There is considerable interest in the role of probiotics in immune function. The objective of this systematic review and meta-analysis was to assess the effects of the consumption of a fermented dairy drink containing Lacticaseibacillus paracasei subsp. paracasei CNCM I-1518 (the previous taxonomic nomenclature was Lactobacillus casei CNCM I-1518, prior to the nomenclature change in April 2020) and the standard yogurt cultures (hereinafter referred to collectively as “FDD”) on common infectious diseases (CIDs) in generally healthy children and adults. Nine literature databases were searched, and nine randomized controlled trials from eight publications were eligible for inclusion. Combined effect sizes were determined for three metrics of CID incidence, two metrics of CID duration, and one metric of CID severity. Compared to the control, the consumption of the FDD resulted in (1) a significant reduction in the odds of experiencing ≥1 CID (odds ratio (OR) (with a 95% confidence interval (CI)): 0.81 (0.66, 0.98); p = 0.029); (2) a significant reduction in mean CIDs per subject (−0.09 (−0.15, −0.04); p = 0.001); and (3) a trend towards reduced risk in cumulative CIDs (relative risk (RR): 0.91 (0.82, 1.01); p = 0.082). The consumption of the FDD had no significant effect on CID duration or severity. Based on the studies conducted thus far, these results suggest that the FDD may reduce CID incidence in the general population.
Biofilm Formation and Expression of Virulence Genes of Microorganisms Grown in Contact with a New Bioactive Glass
Authors: Oliveira Viviane de Cássia , Souza Marina Trevelin , Zanotto Edgar Dutra , Watanabe Evandro , Coraça-Huber Débora
Published: November 2020

Bioactive glass F18 (BGF18), a glass containing SiO2–Na2O–K2O–MgO–CaO–P2O5, is highly effective as an osseointegration buster agent when applied as a coating in titanium implants. Biocompatibility tests using this biomaterial exhibited positive results; however, its antimicrobial activity is still under investigation. In this study we evaluated biofilm formation and expression of virulence-factor-related genes in Candida albicans, Staphylococcus epidermidis, and Pseudomonas aeruginosa grown on surfaces of titanium and titanium coated with BGF18. C. albicans, S. epidermidis, and P. aeruginosa biofilms were grown on specimens for 8, 24, and 48 h. After each interval, the pH was measured and the colony-forming units were counted for the biofilm recovery rates. In parallel, quantitative real-time polymerase chain reactions were carried out to verify the expression of virulence-factor-related genes. Our results showed that pH changes of the culture in contact with the bioactive glass were merely observed. Reduction in biofilm formation was not observed at any of the studied time. However, changes in the expression level of genes related to virulence factors were observed after 8 and 48 h of culture in BGF18. BGF18 coating did not have a clear inhibitory effect on biofilm growth but promoted the modulation of virulence factors.
Revisiting Brucellosis in Small Ruminants of Western Border Areas in Pakistan
Authors: Jamil Tariq , Kasi Khushal Khan , Melzer Falk , Saqib Muhammad , Ullah Qudrat , Khan Muhammad Roidar , Dadar Maryam , Tayyab Muhammad Haleem , Schwarz Stefan , Neubauer Heinrich
Published: November 2020

Brucellosis, globally known bacterial zoonosis, is endemic to Pakistan. B. abortus in bovines, B. melitensis in small ruminants and B. canis in dogs mainly cause this disease. A total of 1821 sera (1196 from sheep and 625 from goats) from animal herds near the Pakistan–Afghanistan border were collected. In parallel testing of sera for anti-Brucella antibodies (B. abortus and B. melitensis) was carried out by RBPT and indirect ELISA. The presence of Brucella DNA in sera was tested by real-time PCR. The overall percentage of seropositive samples was 0.99 (18/1821) by both tests. All positive samples originated from Baluchistan territory which translated into 1.76% (18/1021). None of the positive sera had signals for Brucella DNA and none of sera from goats carried detectable antibodies. Both tests showed an almost perfect agreement with Kappa statistics. The flock size was found to be associated with the presence of anti-Brucella antibodies. The samples of Khyber Pakhtunkhwa (KPK) tested negative in both serological tests and hence were not processed for real-time PCR. The present study shows the presence of anti-Brucella antibodies in sheep in the Baluchistan region of Pakistan. Diagnostic services need to be improved and test and slaughter policies might be implemented for eradication of Brucella infection in these areas. Awareness about the infection is needed at the farmer’s level. Isolation and molecular biology of the isolates could help with understanding the prevailing etiology in a better way.
Disappearance of TBEV Circulation among Rodents in a Natural Focus in Alsace, Eastern France
Authors: Bournez Laure , Umhang Gerald , Moinet Marie , Boucher Jean-Marc , Demerson Jean-Michel , Caillot Christophe , Legras Léo , Devillers Elodie , Hansmann Yves , Velay Aurélie , Richomme Céline , Moutailler Sara , Boué Franck
Published: November 2020

Tick-borne encephalitis virus (TBEV) depends mainly on a fragile mode of transmission, the co-feeding between infected nymphs and larvae on rodents, and thus persists under a limited set of biotic and abiotic conditions. If these conditions change, natural TBEV foci might be unstable over time. We conducted a longitudinal study over seven years in a mountain forest in Alsace, Eastern France, located at the western border of known TBEV distribution. The objectives were (i) to monitor the persistence of TBEV circulation between small mammals and ticks and (ii) to discuss the presence of TBEV circulation in relation to the synchronous activity of larvae and nymphs, to the densities of questing nymphs and small mammals, and to potential changes in meteorological conditions and deer densities. Small mammals were trapped five times per year from 2012 to 2018 to collect blood samples and record the presence of feeding ticks, and were then released. Questing nymphs were collected twice a year. Overall, 1344 different small mammals (Myodes glareolus and Apodemus flavicollis) were captured and 2031 serum samples were tested for the presence of antibodies against TBEV using an in-house ELISA. Seropositive rodents (2.1%) were only found from 2012 to 2015, suggesting that the virus disappeared afterwards. In parallel, we observed unusual variations in inter-annual nymph abundance and intra-annual larval activity that could be related to exceptional meteorological conditions. Changes in the densities of questing nymphs and deer associated with the natural stochastic variations in the frequency of contacts between rodents and infected ticks may have contributed to the endemic fadeout of TBEV on the study site. Further studies are needed to assess whether such events occur relatively frequently in the area, which could explain the low human incidence of TBE in Alsace and even in other areas of France.
Current Targeted Therapies for the Fight against Non-Small Cell Lung Cancer
Authors: Mustachio Lisa , Roszik Jason
Published: November 2020

Lung cancers contribute to the greatest number of cancer-related deaths worldwide and still pose challenges in response to current treatment strategies. Non-small cell lung cancer (NSCLC) accounts for over 85% of lung cancers diagnosed in the United States and novel therapeutics are needed for the treatment of this disease. First and second generation targeted therapies against specific mutated or rearranged oncogenes in NSCLCs show anti-tumor activity and also increase survival. However, many NSCLC patients eventually develop resistance to these therapies or do not properly respond if they have central nervous system metastases. Thus, this review summarizes recent developments and findings related to the generation of novel targeted therapies recently or currently being developed to tackle hurdles that prior therapies were not able to overcome.
Evaluation of VCAM-1 Targeted Naringenin/Indocyanine Green-Loaded Lipid Nanoemulsions as Theranostic Nanoplatforms in Inflammation
Authors: Fuior Elena Valeria , Mocanu Cristina Ana , Deleanu Mariana , Voicu Geanina , Anghelache Maria , Rebleanu Daniela , Simionescu Maya , Calin Manuela
Published: November 2020

Naringenin, an anti-inflammatory citrus flavonoid, is restrained from large-scale use by its reduced water solubility and bioavailability. To overcome these limitations, naringenin was loaded into lipid nanoemulsions directed towards vascular cell adhesion molecule (VCAM)-1, exposed by activated endothelium, and delivered intravenously in a murine model of lipopolysaccharide (LPS)-induced inflammation. To follow the in vivo bio-distribution, naringenin-loaded nanoemulsions were labeled with near-infrared probe Indocyanine Green (ICG). Based on ICG fluorescence, a VCAM-1-dependent retention of nanoemulsions was detected in the heart and aorta, while ultra-high-performance liquid chromatography (UHPLC) measurements showed a target-selective accumulation of naringenin in the heart and lungs. Correlated, fluorescence and UHPLC data indicated a mixed behavior of the VCAM-1 directed nanoparticles, which were driven not only by the targeting moiety but also by passive retention. The treatment with naringenin-loaded nanoemulsions reduced the mRNA levels of some inflammatory mediators in organs harvested from mice with acute inflammation, indicative of their anti-inflammatory potential. The data support a novel theranostic nanoplatform for inflammation, the naringenin/ICG-loaded nanoparticles that either by passive accumulation or effective targeting of the activated endothelium can be employed for imaging inflamed vascular areas and efficient delivery of the encapsulated therapeutic agent.
The Importance of Poly(ethylene glycol) and Lipid Structure in Targeted Gene Delivery to Lymph Nodes by Lipid Nanoparticles
Authors: Zukancic Danijela , Suys Estelle J. A. , Pilkington Emily H. , Algarni Azizah , Al-Wassiti Hareth , Truong Nghia P.
Published: November 2020

Targeted delivery of nucleic acids to lymph nodes is critical for the development of effective vaccines and immunotherapies. However, it remains challenging to achieve selective lymph node delivery. Current gene delivery systems target mainly to the liver and typically exhibit off-target transfection at various tissues. Here we report novel lipid nanoparticles (LNPs) that can deliver plasmid DNA (pDNA) to a draining lymph node, thereby significantly enhancing transfection at this target organ, and substantially reducing gene expression at the intramuscular injection site (muscle). In particular, we discovered that LNPs stabilized by 3% Tween 20, a surfactant with a branched poly(ethylene glycol) (PEG) chain linking to a short lipid tail, achieved highly specific transfection at the lymph node. This was in contrast to conventional LNPs stabilized with a linear PEG chain and two saturated lipid tails (PEG-DSPE) that predominately transfected at the injection site (muscle). Interestingly, replacing Tween 20 with Tween 80, which has a longer unsaturated lipid tail, led to a much lower transfection efficiency. Our work demonstrates the importance of PEGylation in selective organ targeting of nanoparticles, provides new insights into the structure–property relationship of LNPs, and offers a novel, simple, and practical PEGylation technology to prepare the next generation of safe and effective vaccines against viruses or tumours.
Self-Assembling Tacrolimus Nanomicelles for Retinal Drug Delivery
Authors: Gote Vrinda , Mandal Abhirup , Alshamrani Meshal , Pal Dhananjay
Published: November 2020

Neovascular age-related macular degeneration (AMD) is characterized by an increase in reactive oxygen species (ROS) and pro-inflammatory cytokines in the retinal pigment epithelium cells. The primary purpose of this study was the development of a clear, tacrolimus nanomicellar formulation (TAC-NMF) for AMD. The optimized formulation had a mean diameter of 15.41 nm, a zeta potential of 0.5 mV, and an entrapment efficiency of 97.13%. In-vitro cytotoxicity studies revealed the dose-dependent cytotoxicity of TAC-NMF on various ocular cell lines, such as human retinal pigment epithelium (D407), monkey retinal choroidal endothelial (RF/6A) cells, and human corneal epithelium (CCL 20.2) cells. Cellular uptake and in-vitro distribution studies using flow cytometry and confocal microscopy, respectively, indicated an elevated uptake of TAC-NMF in a time-dependent manner. Biocompatibility assay using macrophage RAW 264.7 cell line resulted in low production of inflammatory cytokines such as IL-6, IL-1β and TNF-α after treatment with TAC-NMF. There was a decrease in ROS in D407 cells pre-treated with sodium iodate (ROS inducing agent) after treating with TAC-NMF and tacrolimus drug. Similarly, there was a reduction in the pro-inflammatory cytokines and VEGF-A in D407 cells pretreated with sodium iodate. This indicates that TAC-NMF could lower pro-inflammatory cytokines and ROS commonly seen in AMD.
Bioavailability of the Common Cold Medicines in Jellies for Oral Administration
Authors: Kim Ki Hyun , Jun Minju , Lee Mi-Kyung
Published: November 2020

Jellies for oral administration have been suggested as alternative dosage forms to conventional tablets for improved palatability and compliances for pediatric and geriatric patients. To evaluate the effect of jelly formulation on the bioavailability of cold medicines, two types of jellies were prepared for a fixed-dose combination of acetaminophen (AAP), chlorpheniramine maleate (CPM), dextromethorphan hydrobromide (DMH), and dl-methylephedrine hydrochloride (MEH). Jelly-S and Jelly-H were fabricated using carrageenan and locust bean gum in the absence and presence of xanthan gum, respectively. In vitro dissolution and in vivo absorption of the four drugs in the jellies were compared with other conventional formulations, a syrup and two types of immediate-release (IR) tablets with different hardness, Tablet-S (15 kPa) and Tablet-H (20 kPa). All the formulations exhibited more than 80% dissolution rate within 2 h even though the syrup, Jelly-S, and Tablet-S showed higher 30-min dissolution compared to Jelly-H and Tablet-H. The dissolution rates from the jellies decreased with increasing pH, which resulted in the slowest dissolution in pH 6.8 compared to the syrup and IR tablets. When administered orally to beagle dogs, all five formulations were determined not to be bioequivalent. However, Jelly-S and Jelly-H showed 0.82–1.05 of the geometric mean ratios (GMRs) of AUC0-t for all four drugs compared to the syrup suggesting comparable absorption. In two IR tablets, GMRs of AUC0-t were in a range of 0.55–0.95 indicating a tendency of lower absorption than the syrup and jellies. In conclusion, jelly can be a patient-centered formulation with comparable bioavailability to syrup.
Natural Surfactant Saponin from Tissue of Litsea glutinosa and Its Alternative Sustainable Production
Authors: Wisetkomolmat Jiratchaya , Suksathan Ratchuporn , Puangpradab Ratchadawan , Kunasakdakul Keawalin , Jantanasakulwong Kittisak , Rachtanapun Pornchai , Sommano Sarana Rose
Published: November 2020

In this research, we assessed the detergency properties along with chemical characteristic of the surfactant extracts from the most frequently cited detergent plants in Northern Thailand, namely, Sapindus rarak, Acacia concinna, and Litsea glutinosa. Moreover, as to provide the sustainable option for production of such valuable ingredients, plant tissue culture (PTC) as alternative method for industrial metabolite cultivation was also proposed herein. The results illustrated that detergent plant extracts showed moderate in foaming and detergency abilities compared with those of synthetic surfactant. The phytochemical analysis illustrated the positive detection of saponins in L. glutinosa plant extracts. The highest callus formation was found in L. glutinosa explant cultured with MS medium supplemented with 2.0 mg/L Indole-3-acetic acid (IAA). The callus extract was chemical elucidated using chromatography, which illustrated the presence of saponin similar to those from the crude leaf and Quillaja saponin extracts. Compact mass spectrometry confirmed that the surfactant was of the steroidal diagnostic type.
Screening of Salt Stress Responsive Genes in Brachypodium distachyon (L.) Beauv. by Transcriptome Analysis
Authors: Guo Xiuxiu , Wang Qingjun , Liu Yuan , Zhang Xuejie , Zhang Luoyan , Fan Shoujin
Published: November 2020

As one of the most common abiotic stresses, salt stress seriously impairs crop yield. Brachypodium distachyon (L.) Beauv. is a model species for studying wheat and other grasses. In the present investigation, the physiological responses of B. distachyon treated with different concentrations of NaCl for 24 h were measured. Therefore, the control and the seedlings of B. distachyon treated with 200 mM NaCl for 24 h were selected for transcriptome analysis. Transcriptome differential analysis showed that a total of 4116 differentially expressed genes (DEGs) were recognized, including 3120 upregulated and 996 downregulated ones. GO enrichment assay indicated that some subsets of genes related to the active oxygen scavenging system, osmoregulatory substance metabolism, and abscisic-acid (ABA)-induced stomatal closure were significantly upregulated under salt stress. The MapMan analysis revealed that the upregulated genes were dramatically enriched in wax metabolic pathways. The expressions of transcription factor (TF) family members such as MYB, bHLH, and AP2/ERF were increased under salt stress, regulating the response of plants to salt stress. Collectively, these findings provided valuable insights into the mechanisms underlying the responses of grass crops to salt stress.