bluuebunny/crossref_metadata_2025 huggingface dataset:
Characterization, Calcination and Pre-Reduction of Polymetallic Manganese Nodules by Hydrogen and Methane
Authors: Brustad Ole Kristian , Låstad Jonas , Hoseinpur Arman , Safarian Jafar
Published: November 2022

A polymetallic manganese nodule was characterized and further calcined and pre-reduced by H2, CH4, and H2-CH4 mixtures at elevated temperatures. It was found that the main Mn and Fe elements coexisted in the ore in different minerals, and the Mn/Fe ratio varies in the ore. Moreover, Cu, Ni and Co are distributed with Mn and Fe, and no known minerals of these elements were identified. The calcination of the ore was carried out through calcination in air in a muffle furnace, and under Ar in a thermogravimetry (TG) furnace. It was found that manganese and iron oxides are evolved from a variety of oxide and hydroxides in the ore during calcination. The pre-reduction of the calcined ore particles by H2 gas in the TG furnace indicated fast reduction by hydrogen. The pre-reduction of the calcined ore by H2, CH4, and H2-CH4 mixtures in a stationary bed reactor and further characterization of the products indicated the same products. It was found that the pre-reduction by all the applied gases at elevated temperatures yield a pre-reduced ore that contains metallic Fe, Cu, Ni, and Co, while MnO co-exist as the dominant phase.
A Comprehensive Mass Spectrometry-Based Workflow for Clinical Metabolomics Cohort Studies
Authors: Shi Zhan , Li Haohui , Zhang Wei , Chen Youxiang , Zeng Chunyan , Kang Xiuhua , Xu Xinping , Xia Zhenkun , Qing Bei , Yuan Yunchang , Song Guodong , Caldana Camila , Hu Junyuan , Willmitzer Lothar , Li Yan
Published: November 2022

As a comprehensive analysis of all metabolites in a biological system, metabolomics is being widely applied in various clinical/health areas for disease prediction, diagnosis, and prognosis. However, challenges remain in dealing with the metabolomic complexity, massive data, metabolite identification, intra- and inter-individual variation, and reproducibility, which largely limit its widespread implementation. This study provided a comprehensive workflow for clinical metabolomics, including sample collection and preparation, mass spectrometry (MS) data acquisition, and data processing and analysis. Sample collection from multiple clinical sites was strictly carried out with standardized operation procedures (SOP). During data acquisition, three types of quality control (QC) samples were set for respective MS platforms (GC-MS, LC-MS polar, and LC-MS lipid) to assess the MS performance, facilitate metabolite identification, and eliminate contamination. Compounds annotation and identification were implemented with commercial software and in-house-developed PAppLineTM and UlibMS library. The batch effects were removed using a deep learning model method (NormAE). Potential biomarkers identification was performed with tree-based modeling algorithms including random forest, AdaBoost, and XGBoost. The modeling performance was evaluated using the F1 score based on a 10-times repeated trial for each. Finally, a sub-cohort case study validated the reliability of the entire workflow.
Comparative Transcriptome Analysis of Two Chrysomycin-Producing Wild-Type and Mutant Strains of Streptomyces sp. 891
Authors: Zhu Wangjie , Pei Xinwei , Chen Xiaoyu , Wu You , Song Fuhang , Zhang Huawei
Published: November 2022

Chrysomycin A (CA), a promising antibiotic agent, usually coexists with two analog chrysomycins B (CB) and C (CC) produced by several wild-type (WT) Streptomyces strains. With the aim to increase CA production, UV mutagenesis-based breeding had been employed on a marine-derived strain Streptomyces sp. 891 in our previous study and afforded an improved strain 891-B6 with enhanced CA yield. By comparative transcriptome analysis, significant differences in chrysomycin BGC-related gene expression between the WT strain 891 and the mutant strain 891-B6 were unveiled in the current study. Among 25 up-regulated genes in mutant 891-B6, chryA, chryB, chryC, chryF, chryG, chryK, chryP, and chryQ, responsible for the biosynthesis of benzonaphthopyranone aglycone, and chryD, chryE, and chryU in charge of production of its deoxyglycoside, were characterized. Furthermore, the expression of genes chryOII, chryOIII, and chryOIV responsible for the formation of 8-vinyl in CA from 8-ethyl in CB were greatly enhanced in strain 891-B6. These findings provide molecular mechanisms for increased yield of CA and decreased yield of CB for mutant 891-B6, which has potential application in industrial CA production.
The Role of Sphingolipids in the Pathogenesis of Psoriasis
Authors: Matwiejuk Mateusz , Mysliwiec Hanna , Chabowski Adrian , Flisiak Iwona
Published: November 2022

Psoriasis is a complex, chronic, immunologically mediated disease which involves skin and joints. Psoriasis is commonly connected with numerous other diseases such as liver diseases, metabolic syndrome, impaired glucose tolerance, diabetes mellitus, atherosclerosis, hypertension, and ischemic heart disease. Interestingly, comorbidities of psoriasis are an attention-grabbing issue. Additionally, it can cause impairment of quality of life and may be associated with depressive disorders. Altered levels of ceramides in psoriatic skin may lead to anti-apoptotic and pro-proliferative states, consequently leading to an over-proliferation of keratinocytes and the development of skin lesions. The pathophysiology of psoriasis and its comorbidities is not fully understood yet. Sphingolipids (including ceramides) and their disturbed metabolism may be the link between psoriasis and its comorbidities. Overall, the goal of this review was to discuss the role of sphingolipid disturbances in psoriasis and its comorbidities. We searched the PubMed database for relevant articles published before the beginning of May 2022. The systematic review included 65 eligible original articles.
A Wind-Driven Rotating Micro-Hybrid Nanogenerator for Powering Environmental Monitoring Devices
Authors: Zhu Yongqiang , Zhao Yu , Hou Lijun , Zhang Pingxia
Published: November 2022

In recent years, environmental problems caused by natural disasters due to global warming have seriously affected human production and life. Fortunately, with the rapid rise of the Internet of Things (IoT) technology and the decreasing power consumption of microelectronic devices, it is possible to set up a multi-node environmental monitoring system. However, regular replacement of conventional chemical batteries for the huge number of microelectronic devices still faces great challenges, especially in remote areas. In this study, we developed a rotating hybrid nanogenerator for wind energy harvesting. Using the output characteristics of triboelectric nanogenerator (TENG) with low frequency and high voltage and electromagnetic generator (EMG) with high frequency and high current, we are able to effectively broaden the output voltage range while shortening the capacitor voltage rising time, thus obtaining energy harvesting at wide frequency wind speed. The TENG adopts the flexible contact method of arch-shaped film to solve the problem of insufficient flexible contact and the short service life of the rotating triboelectric generator. After 80,000 cycles of TENG operation, the maximum output voltage drops by 7.9%, which can maintain a good and stable output. Through experimental tests, the maximum output power of this triboelectric nanogenerator is 0.55 mW at 400 rpm (wind speed of about 8.3 m/s) and TENG part at an external load of 5 MΩ. The maximum output power of the EMG part is 15.5 mW at an external load of 360 Ω. The hybrid nanogenerator can continuously supply power to the anemometer after running for 9 s and 35 s under the simulated wind speed of 8.3 m/s and natural wind speed of 5.6 m/s, respectively. It provides a reference value for solving the power supply problem of low-power environmental monitoring equipment.
Rubik’s Cube as Reconfigurable Microfluidic Platform for Rapid Setup and Switching of Analytical Devices
Authors: Lai Xiaochen , Sun Yanfei , Yang Mingpeng , Wu Hao
Published: November 2022

Microfluidics technology plays an important role in modern analytical instruments, while the modular design of microfluidics facilitates the reconfiguration of analytical instrument functions, making it possible to deploy on-demand systems in the field. However, modular design also faces the challenges such as connection reliability and reconfiguration convenience. Inspired by the self-locking structure of the Rubik’s cube, a modular, reconfigurable microfluidic instrument architecture is proposed in this paper. The system has a self-locking structure of Rubik’s cube components and an O-ring-based alignment and sealing mechanism, which enables reliable interconnection and rapid rearrangement of microfluidic modules by simply rotating the faces of the microfluidic cube. In addition, the system is capable of integrating a variety of customized modules to perform analysis tasks. A proof-of-concept application of detecting multiple pollutants in water is demonstrated to show the reconfigurable characteristics of the system. The findings of this paper provide a new idea for the design of microfluidic analytical instrument architectures.
Simulation of an Ionic Rare Earth Leaching Process Based on the Darcy Law-Chemical Reaction Engineering-Transfer of Dilute Substance Coupling
Authors: Wang Dan , Wu Fuyu , Rao Yunzhang , Xu Wei , Han Min , Shi Liang
Published: November 2022

The basic principle of in situ leaching is chemical mining. The process of in situ leaching is to inject leaching solution into the ore body, and the leaching solution is spread in the pores of the mountain. The process is completed by the coupling action of the liquid seepage field and ion exchange reactions. In the production process, only one injection of liquid can be carried out in a certain stope, so it is impossible to improve the injection process and leaching effect through field practice. By simulating the in situ leaching process of rare earth ions, this paper builds the test stope true three-dimensional numerical model and simulates the leaching process of rare earth ore under the coupling of seepage control, ion exchange, and dilute material transfer in porous media. The migration rule of RE3+ and Mg2+ in stopes was analyzed to evaluate the leaching effect. It is of great significance to increase the recovery rate of rare earth ore.
Synthesis and Characterization of Poly(lactic acid) Composites with Organosolv Lignin
Authors: Terzopoulou Zoi , Xanthopoulou Eleftheria , Pardalis Nikolaos , Pappa Christina P. , Torofias Stylianos , Triantafyllidis Konstantinos S. , Bikiaris Dimitrios N.
Published: November 2022

Lignin, being one of the main structural components of lignocellulosic biomass, is considered the most abundant natural source of phenolics and aromatics. Efforts for its valorisation were recently explored as it is mostly treated as waste from heat/energy production via combustion. Among them, polymer-based lignin composites are a promising approach to both valorise lignin and to fine tune the properties of polymers. In this work, organosolv lignin, from beech wood, was used as fillers in a poly (lactic acid) (PLA) matrix. The PLA/lignin composites were prepared using melt mixing of masterbatches with neat PLA in three different lignin contents: 0.5, 1.0 and 2.5 wt%. Lignin was used as-isolated, via the organosolv biomass pretreatment/fractionation process and after 8 h of ball milling. The composites were characterised with Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) spectroscopy, X-ray Diffraction (XRD), and Differential Scanning Calorimetry (DSC). Additionally, their antioxidant activity was assessed with the 2,2-Diphenyil-1-picrylhydrazyl (DPPH) method, the colour was measured with a colorimeter and the mechanical properties were evaluated with tensile testing. Ball milling, at least under the conditions applied in this study, did not induce a further substantial decrease in the already relatively small organosolv lignin primary particles of ~1 μm. All the produced PLA/lignin composites had a uniform dispersion of lignin. Compression-moulded films were successfully prepared, and they were coloured brown, with ball-milled lignin, giving a slightly lighter colour in comparison with the as-received lignin. Hydrogen bonding was detected between the components of the composites, and crystallization of the PLA was suppressed by both lignin, with the suppression being less pronounced by the ball-milled lignin. All composites showed a significantly improved antioxidant activity, and their mechanical properties were maintained for filler content 1 wt%.
Edible Mushrooms as Source of Fibrin(ogen)olytic Enzymes: Comparison between Four Cultivated Species
Authors: Petraglia Tania , Latronico Tiziana , Liuzzi Grazia Maria , Fanigliulo Angela , Crescenzi Aniello , Rossano Rocco
Published: November 2022

Cardiovascular diseases represent the main cause of death. A common feature of cardiovascular disease is thrombosis resulting from intravascular accumulation of fibrin. In the last years, several fibrinolytic enzymes have been discovered in many medicinal or edible mushrooms as potential new antithrombotic agents. This study aimed to compare the fibrin(ogen)olytic activity of crude extracts from the fruiting bodies of four cultivated edible mushrooms: Lentinula edodes, Pleurotus ostreatus, Pleurotus eryngii, and Agrocybe aegerita. Fibrin(ogen)olytic activity was assessed by fibrin plate, spectrophotometric assay and electrophoretic analysis (SDS-PAGE and zymography). The highest activity was detected for P. ostreatus followed by P. eryngii, L. edodes and A. aegerita. Results indicated that enzymes exhibited maximum activity at pH 6–7 and 30–40 °C, respectively. Enzyme activity was inhibited by serine and metalloprotease inhibitors. We proposed a new index called the Specific Fibrin(ogen)olytic Index (SFI), which allows specification of the proportion of the total proteolytic capacity due to the fibrin(ogen)olytic activity. These data suggest that the extracts from fruiting bodies or powdered mushrooms can be used as functional ingredients for the development of new functional foods that may act as thrombolytic agents responding, at the same time, to the increasing demand for safe, healthy and sustainable food.
Inhibition of Phytopathogenic and Beneficial Fungi Applying Silver Nanoparticles In Vitro
Authors: Vera-Reyes Ileana , Altamirano-Hernández Josué , Reyes-de la Cruz Homero , Granados-Echegoyen Carlos A. , Loera-Alvarado Gerardo , López-López Abimael , Garcia-Cerda Luis A. , Loera-Alvarado Esperanza
Published: November 2022

In the current research, our work measured the effect of silver nanoparticles (AgNP) synthesized from Larrea tridentata (Sessé and Moc. ex DC.) on the mycelial growth and morphological changes in mycelia from different phytopathogenic and beneficial fungi. The assessment was conducted in Petri dishes, with Potato-Dextrose-Agar (PDA) as the culture medium; the AgNP concentrations used were 0, 60, 90, and 120 ppm. Alternaria solani and Botrytis cinerea showed the maximum growth inhibition at 60 ppm (70.76% and 51.75%). Likewise, Macrophomina spp. required 120 ppm of AgNP to achieve 65.43%, while Fusarium oxisporum was less susceptible, reaching an inhibition of 39.04% at the same concentration. The effect of silver nanoparticles was inconspicuous in Pestalotia spp., Colletotrichum gloesporoides, Phytophthora cinnamomi, Beauveria bassiana, Metarhizium anisopliae, and Trichoderma viridae fungi. The changes observed in the morphology of the fungi treated with nanoparticles were loss of definition, turgidity, and constriction sites that cause aggregations of mycelium, dispersion of spores, and reduced mycelium growth. AgNP could be a sustainable alternative to managing diseases caused by Alternaria solani and Macrophomina spp.
Effect of Phytosynthesized Selenium and Cerium Oxide Nanoparticles on Wheat (Triticum aestivum L.) against Stripe Rust Disease
Authors: Shahbaz Muhammad , Fatima Noor , Mashwani Zia-ur-Rehman , Akram Abida , Haq Ehsan ul , Mehak Asma , Abasi Fozia , Ajmal Maryam , Yousaf Tayyaba , Raja Naveed Iqbal , UlHassan Hammad , Pérez de la Lastra José Manuel
Published: November 2022

In this study, selenium nanoparticles (SeNPs) and cerium oxide nanoparticles (CeONPs) were synthesized by using the extract of Melia azedarach leaves, and Acorus calamusas rhizomes, respectively, and investigated for the biological and sustainable control of yellow, or stripe rust, disease in wheat. The green synthesized NPs were characterized by UV-Visible spectroscopy, scanning electron microscopy (SEM), energy-dispersive X-ray (EDX), and X-ray diffraction (XRD). The SeNPs and CeONPs, with different concentrations (i.e., 10, 20, 30, and 40 mg/L), were exogenously applied to wheat infected with Puccinia striformis. SeNPs and CeONPs, at a concentration of 30 mg/L, were found to be the most suitable concentrations, which reduced the disease severity and enhanced the morphological (plant height, root length, shoot length, leaf length, and ear length), physiological (chlorophyll and membrane stability index), biochemical (proline, phenolics and flavonoids) and antioxidant (SOD and POD) parameters. The antioxidant activity of SeNPs and CeONPs was also measured. For this purpose, different concentrations (50, 100, 150, 200 and 400 ppm) of both SeNPs and CeONPs were used. The concentration of 400 ppm most promoted the DPPH, ABTS and reducing power activity of both SeNPs and CeONPs. This study is considered the first biocompatible approach to evaluate the potential of green synthesized SeNPs and CeONPs to improve the health of yellow, or stripe rust, infected wheat plants and to provide an effective management strategy to inhibit the growth of Puccinia striformis.
Resin Glycosides from Convolvulaceae Family: An Update
Authors: Maharani Rani , Fajar Mohamad , Supratman Unang
Published: November 2022

Resin glycoside is a type of secondary metabolite isolated commonly from the Convolvulaceae family. It consists of oligosaccharides conjugated to organic acids with a larger percentage having a macrocyclic structure. The resin glycosides reported in this review is classified mostly based on the number of sugar units constructing the structure, which is correlated to the biological properties of the compounds. According to preliminary reviews, the protocols to isolate the compounds are not straightforward and require a special technique. Additionally, the structural determination of the isolated compounds needs to minimize the structure for the elucidation to become easier. Even though resin glycosides have a complicated structural skeleton, several total syntheses of the compounds have been reported in articles published from 2010 to date. This review is an update on the prior studies of the resin glycosides reported in 2010 and 2017. The review includes the classification, isolation techniques, structural determination, biological properties, and total synthesis of the resin glycosides.
Sustainable and Efficacy Approach of Green Synthesized Cobalt Oxide (Co3O4) Nanoparticles and Evaluation of Their Cytotoxicity Activity on Cancerous Cells
Authors: Al-Qasmi Noha
Published: November 2022

In this study, rosemary leaf extract was effectively used to synthesize cobalt oxide nanoparticles (Co3O4 NPs) using a rapid, low-cost, and environmentally friendly approach. The prepared Co3O4 NPs were examined using various analytical techniques. However, UV spectrum analysis displayed two sharp absorption peaks at ~350 and 745 nm. The dynamic light scattering and zeta potential measurements were used to evaluate the particle size and the effective stabilization of the synthetic nanoparticles in the suspensions. A semi-triangular pyramidal shape of the Co3O4 NPs with a wide particle-size distribution could be observed in the scanning electron microscopy images. The energy-dispersive X-ray spectrum confirmed their successful synthesis, as the experimental atomic percentages agreed with the theoretical values. Moreover, X-ray diffraction analysis revealed that the synthesized Co3O4 NPs had a cubic crystalline structure corroborating that of theoretical Co3O4. Additionally, the Co3O4 NPs were not toxic at ≤62.5 µg/mL for Hep G2 and at ≤31.25 µg/mL for Mcf7. Therefore, these unique environmentally friendly Co3O4 NPs at this safe concentration could be studied in the future for their therapeutic activity.
Luminous Self-Assembled Fibers of Azopyridines and Quantum Dots Enabled by Synergy of Halogen Bond and Alkyl Chain Interactions
Authors: Pan Ying , Xue Lulu , Chen Yinjie , Hu Yingjie , Sun Zhicheng , Mo Lixin , Li Luhai , Yu Haifeng
Published: November 2022

Herein, a simple approach for the fabrication of luminous self-assembled fibers based on halogen-bonded azopyridine complexes and oleic acid-modified quantum dots (QDs) is reported. The QDs uniformly align on the edge of the self-assembled fibers through the formation of van der Waals force between the alkyl chain of oleic acid on the QD surface and the alkyl chain of the halogen-bonded complexes, 15Br or 15I. Furthermore, the intermolecular interaction mechanism was elucidated by using Fourier-transform infrared spectroscopy (FTIR), Raman spectroscopy, and density functional theory (DFT) calculations. This approach results in retention of the fluorescence properties of the QDs in the fibers. In addition, the bromine-bonded fibers can be assembled into tailored directional fibers upon evaporation of the solvent (tetrahydrofuran) when using capillaries via the capillary force. Interestingly, the mesogenic properties of the halogen-bonded complexes are preserved in the easily prepared halogen-bonded fluorescent fibers; this provides new insight into the design of functional self-assembly materials.
DBU Promoted Polysubstituted Arene Formation via a Michael Addition/Cyclization/Elimination Cascade Reaction
Authors: Bai Guishun , Yang Yang , Wang Xingyue , Wu Jiamin , Wang Hong , Ye Xinyi , Bao Xiaoze
Published: November 2022

The straightforward construction of polysubstituted arenes is essential in both synthetic chemistry and medicinal chemistry. Herein, we reported a DBU promoted Michael addition/cyclization/elimination cascade reaction between vinylogous malononitrile derivatives and chlorinated nitrostyrenes for the synthesis of polysubstituted arenes. The method features mild reaction conditions, wide substrate scope and high yield. Interestingly, preliminary study of the enantioselective version of this cascade was conducted to give chiral biaryl atropisomers with up to 40% ee through center-to-axial chirality transfer strategy.
Substituent Effects on the Ultraviolet Absorption Properties of 2,4-Dihydroxy Dibenzophenone
Authors: Wu Feng , Tan Shengqiong , Fang Zhengjun , Deng Jiyu , He Zhengjie , Huang Chaoyi , Au Chaktong , Yi Bing
Published: November 2022

Substituent effects on the ultraviolet absorption properties of 2,4-dihydroxy dibenzophenone were investigated experimentally. Nine compounds of 2,4-dihydroxy dibenzophenone with different substituents were prepared by a solvent-free reaction of benzoyl chloride. The maximum absorption wavelength (λmax) of these samples was measured, and their UV resistance properties in cotton fabric as well as in polyester were determined. The results show that the λmax is dependent on the substituents at the benzylidene ring, and both electron donating substituents and electron withdrawing substituents cause a bathochromic shift. The UV resistance of fabric increases with the increase in compound concentration. The dyeing rate of each compound on polyester was higher than that of cotton. On cotton fabric, the dyeing rate of 2,4-dihydroxybenzophenone was the highest, 77.8%. On polyester, that of 2,4-dihydroxy-4′-ethyl dibenzophenone was the highest, 84.1%. The study provides new insights into the effect of substituents on the properties of 2,4-dihydroxy dibenzophenone that are related to the whitening of cotton and polyester materials.
Fast and Sensitive Determination of Cadmium and Selenium in Rice by Direct Sampling Electrothermal Vaporization Inductively Coupled Plasma Mass Spectrometry
Authors: Lan Guanyu , Li Xue , Jia Hongyu , Yu Xiaofeng , Wang Zhaohui , Yao Jijun , Mao Xuefei
Published: November 2022

In this work, a direct solid sampling device based on modified graphite furnace electrothermal vaporization (GF-ETV) with inductively coupled plasma mass spectrometry (ICP-MS) was established for the simultaneous detection of trace selenium and cadmium in rice samples. A bypass gas was first designed in GF-ETV to improve the device’s analytical sensitivity and precision. The ashing and vaporization conditions, the flow rates of the Ar carrier and the bypass gases of ICP-MS were all investigated. Under the optimized conditions, the limits of detection (LODs) for Se and Cd were 0.5 μg kg−1 and 0.16 μg kg−1, respectively; the relative standard deviations (RSDs) of repeated measurements were within 8% (n = 6). The recoveries of Cd and Se in rice samples were in the range of 89–112% compared with the microwave digestion ICP-MS method, indicating good accuracy and precision for the simultaneous detection of Se and Cd in rice matrix. The whole analysis time is <3 min without the sample digestion process, fulfilling the fast detection of Se and Cd in rice samples to protect food safety.
Effects of EOs vs. Antibiotics on E. coli Strains Isolated from Drinking Waters of Grazing Animals in the Upper Molise Region, Italy
Authors: Aquilano Chiara , Baccari Ligia , Caprari Claudio , Divino Fabio , Fantasma Francesca , Saviano Gabriella , Ranalli Giancarlo
Published: November 2022

The health and safety of grazing animals was the subject of microbiological monitoring on natural source of drinking waters in the upper Molise region, Italy. Surface water samples, on spring-summer season, were collected and submitted to analyses using sterile membrane filtration, cultural medium, and incubation. The level of environmental microbial contamination (Total viable microbial count, yeasts and fungi) and faecal presence (Total and faecal coliforms, E. coli, and Salmonellae spp.) were carried out. By the selective microbiological screening, twenty-three E. coli strains from drinking waters were isolated and submitted to further studies to evaluate antibiotic resistance by antibiograms vs. three animal and two diffuse human antibiotics. Furthermore, after a fine chemical characterization by GC and GC-MS, three Essential Oils (EOs) of aromatic plants (Timus vulgaris, Melaleuca alternifolia, Cinnamomun verum) aromatograms were performed and results statistically compared. The effects of EOs vs. antibiotics on E. coli strains isolated from drinking waters showed a total absence of microbial resistance. In our experimental conditions, even if some suggestions will be further adopted for better managements of grazing animals, because the health and safety represent a guarantee for both animals and humans.
Surfactants, Biosurfactants, and Non-Catalytic Proteins as Key Molecules to Enhance Enzymatic Hydrolysis of Lignocellulosic Biomass
Authors: Sánchez-Muñoz Salvador , Balbino Thércia R. , de Oliveira Fernanda , Rocha Thiago M. , Barbosa Fernanda G. , Vélez-Mercado Martha I. , Marcelino Paulo R. F. , Antunes Felipe A. F. , Moraes Elisangela J. C. , dos Santos Julio C. , da Silva Silvio S.
Published: November 2022

Lignocellulosic biomass (LCB) has remained a latent alternative resource to be the main substitute for oil and its derivatives in a biorefinery concept. However, its complex structure and the underdeveloped technologies for its large-scale processing keep it in a state of constant study trying to establish a consolidated process. In intensive processes, enzymes have been shown to be important molecules for the fractionation and conversion of LCB into biofuels and high-value-added molecules. However, operational challenges must be overcome before enzyme technology can be the main resource for obtaining second-generation sugars. The use of additives is shown to be a suitable strategy to improve the saccharification process. This review describes the mechanisms, roles, and effects of using additives, such as surfactants, biosurfactants, and non-catalytic proteins, separately and integrated into the enzymatic hydrolysis process of lignocellulosic biomass. In doing so, it provides a technical background in which operational biomass processing hurdles such as solids and enzymatic loadings, pretreatment burdens, and the unproductive adsorption phenomenon can be addressed.
Extreme Ultraviolet Lighting Using Carbon Nanotube-Based Cold Cathode Electron Beam
Authors: Yoo Sung Tae , Park Kyu Chang
Published: November 2022

Laser-based plasma studies that apply photons to extreme ultraviolet (EUV) generation are actively being conducted, and studies by direct electron irradiation on Sn for EUV lighting have rarely been attempted. Here, we demonstrate a novel method of EUV generation by irradiating Sn with electrons emitted from a carbon nanotube (CNT)-based cold cathode electron beam (C-beam). Unlike a single laser source, electrons emitted from about 12,700 CNT emitters irradiated the Sn surface to generate EUV and control its intensity. EUV light generated by direct irradiation of electrons was verified using a photodiode equipped with a 150 nm thick Zr filter and patterning of polymethyl methacrylate (PMMA) photoresist. EUV generated with an input power of 6 W is sufficient to react the PMMA with exposure of 30 s. EUV intensity changes according to the anode voltage, current, and electron incident angle. The area reaching the Sn and penetration depth of electrons are easily adjusted. This method could be the cornerstone for advanced lithography for semiconductor fabrication and high-resolution photonics.
Microstructural Analysis of Organic-Rich Shales: Insights from an Electron Microscopic Study by Application of FIBSEM and TEM
Authors: Han Jinxuan , Zhu Hongjian , Lu Yanjun , Yang Su , Yang Manping , Shi Erxiu , Qi Yu
Published: November 2022

Matrix-related pores play a significant role in controlling hydrocarbon production in organic-rich shales. Multiple matrix-related pore types of typical marine shales in the Sichuan Basin have been visually investigated and identified by transmission electron microscopy (TEM) on ultra-thin sections and by focused ion beam-scanning electron microscopy (FIBSEM) on polished sections. OM-hosted pores seem universal and range in sizes from below 1 nm to hundreds of nanometers and they are not homogeneously developed and distributed, which is mainly determined by thermal maturity and OM composition. Mineral-hosted pores are defined by mineral frameworks and occur in open spaces related to ductile or rigid grain fabric. The four porous mineral types that occur are clay intrapores, carbonate solvopores, pyrite interpores, and quartz interpores, and they range in size from less than 1 nm to more than several microns. Aggregate-hosted pores are predominantly associated with clay-organic aggregates, pyrite-organic aggregates, clay-pyrite aggregates, and clay-organic-pyrite aggregates. The most common aggregate-hosted pore networks are defined by clay-organic aggregates, and the pores are largely developed between the clay and organic layers and may be the important adsorption spaces for methane. Fracture-related pores include microchannels and microfractures of various sizes and shapes and they could play a key role in providing hydrocarbon migration pathways. FIBSEM and TEM show direct evidence that OM-hosted pores and fracture-related pores contribute more to the effective pore network and the excellent reservoir quality, whereas poor reservoir quality may come from aggregate-hosted pores.
Microcrystallization Effects Induced by Laser Annealing in Cr-Al-C Ion-Beam-Sputtered Films
Authors: Crisan Ovidiu , Crisan Alina Daniela
Published: November 2022

The microcrystallization effects induced by the real-time laser annealing in Cr-Al-C ion-sputtered films with an off-stoichiometric composition are studied. The laser annealing has been performed during Raman experiments with tunable laser power densities. Morphostructural changes induced during laser annealing were investigated by scanning electron microscopy. It has been proven that real-time laser annealing in the high-laser-power-density mode promotes quite clearly the formation of nanograins through surface microcrystallization. Detailed Raman analysis allowed for the observation of the optical modes that unequivocally identifies the low-symmetry 211 MAX phase in both low- and high-power-density modes. Such findings confirming the microcrystallization as well as the stabilization of the grain boundaries by carbon nanoclustering are confirmed by X-ray diffraction results, where the single-phase hexagonal 211 was unequivocally proven to form in the high-laser-power-density mode. The microcrystallization via laser annealing was also found to be beneficial for the elastic behavior, as the hardness values between 16 and 26 GPa were found after laser annealing, accompanied by a significantly high Young’s bulk modulus. Such large values, larger than those in bulk compounds, are explicable by the nanometric grain sizes accompanied by the increase of the grain boundary regions.
Diallyl Disulfide Induces Chemosensitization to Sorafenib, Autophagy, and Cell Cycle Arrest and Inhibits Invasion in Hepatocellular Carcinoma
Authors: Machado Ana Rita Thomazela , Tuttis Katiuska , Santos Patrick Wellington da Silva , Aissa Alexandre Ferro , Antunes Lusânia Maria Greggi
Published: November 2022

Hepatocellular carcinoma is the seventh most common type of cancer in the world, with limited treatment options. A promising strategy to treat cancer is to associate chemotherapeutics and plant bioactive compounds. Here, we examined whether diallyl disulfide (DADS; 50–200 μM) and sorafenib (SORA; 8 μM), either alone or in combination, were toxic to hepatocellular carcinoma cells (HepG2) in vitro. We assessed whether DADS and/or SORA induced cell death (LIVE/DEAD assay and autophagy) and cell cycle changes (flow cytometry), altered expression of key genes and proteins (RT-qPCR and Western blot), and modulated tumorigenesis signatures, such as proliferation (clonogenic assay), migration (wound healing), and invasion (inserts). The DADS + SORA combination elicited autophagic cell death by upregulating LC3 and NRF2 expression and downregulating FOS and TNF expression; induced the accumulation of cells in the G1 phase which thereby upregulated the CHEK2 expression; and inhibited invasion by downregulating the MMP2 expression. Predictive analysis indicated the participation of the MAPK pathway in the reported results. The DADS + SORA combination suppressed both cell invasion and clonogenic survival, which indicated that it dampened tumor growth, proliferation, invasion, and metastatic potential. Therefore, the DADS + SORA combination is a promising therapy to develop new clinical protocols.
Metal Coordination and Biological Screening of a Schiff Base Derived from 8-Hydroxyquinoline and Benzothiazole
Authors: Ribeiro Nádia , Farinha Pedro F. , Pinho Jacinta O. , Luiz Hugo , Mészáros János P. , Galvão Adelino M. , Costa Pessoa João , Enyedy Éva A. , Reis Catarina Pinto , Correia Isabel , Gaspar Maria Manuela
Published: November 2022

Designing new metallodrugs for anticancer therapy is a driving force in the scientific community. Aiming to contribute to this field, we hereby report the development of a Schiff base (H2L) derived from the condensation of 2-carbaldehyde-8-hydroxyquinoline with 2-hydrazinobenzothiazole and its complexation with transition metal ions. All compounds were characterised by analytical and spectroscopic techniques, which disclosed their structure: [Cu(HL)Cl], [Cu(HL)2], [Ni(HL)(acetate)], [Ni(HL)2], [Ru(HL)Cl(DMSO)], [VO(HL)2] and [Fe(HL)2Cl(H2O)]. Different binding modes were proposed, showing the ligand’s coordination versatility. The ligand proton dissociation constants were determined, and the tested compounds showed high lipophilicity and light sensitivity. The stability of all complexes in aqueous media and their ability to bind to albumin were screened. Based on an antiproliferative in vitro screening, [Ni(HL)(acetate)] and [Ru(HL)Cl(DMSO)] were selected for further studies aiming to investigate their mechanisms of action and therapeutic potential towards colon cancer. The complexes displayed IC50 < 21 μM towards murine (CT-26) and human (HCT-116) colon cancer cell lines. Importantly, both complexes exhibited superior antiproliferative properties compared to the clinically approved 5-fluorouracil. [Ni(HL)(acetate)] induced cell cycle arrest in S phase in CT-26 cells. For [Ru(HL)Cl(DMSO)] this effect was observed in both colon cancer cell lines. Additionally, both compounds significantly inhibited cell migration particularly in the human colon cancer cell line, HCT-116. Overall, the therapeutic potential of both metal complexes was demonstrated.
Active Films Based on Starch and Wheat Gluten (Triticum vulgare) for Shelf-Life Extension of Carrots
Authors: Rivera Leiva Andrés Felipe , Hernández-Fernández Joaquín , Ortega Toro Rodrigo
Published: November 2022

The use of biodegradable biopolymers with the incorporation of active ingredients has been considered as an alternative to extend the useful life of food. Therefore, the objective of this research was to develop active films based on starch and wheat gluten, containing cinnamon and turmeric essential oils by using the solvent casting method. Different film formulations were made from wheat starch, gluten, glycerol, and essential oils of cinnamon and turmeric. The films were characterized according to their morphology, optical, thermal, antioxidant, and barrier properties. Subsequently, the active properties on baby carrots regarding weight loss, appearance, and fungal growth were evaluated. The results indicated that the starch-based films showed a slight decrease in moisture content with the addition of essential oils (up to 13.29%), but at the same time showed a significant reduction in water solubility (up to 28.4%). Gluten-based films did not present significant differences in these parameters, although the solubility in water tended to increase (up to 13.15%) with the addition of essential oils. In general, the films presented good thermal stability and antioxidant capacity, and in the carrot coating test, a decrease in weight loss of up to 44.44% and 43.33% was observed for the coatings based on starch and gluten with the addition of turmeric essential oil, respectively. Finally, films developed with cinnamon and turmeric essential oils are potential candidates for the design of biodegradable active packaging.
A Polysiloxane Delivery Vehicle of Cyclic N-Halamine for Biocidal Coating of Cellulose in Supercritical CO2
Authors: Li Leixuan , Xin Yan , Wu Fengze , Lyu Xiangrong , Yao Qiyuan , Yin Xiaoting , Zhang Qiang , Shan Wenjuan , Chen Yong , Han Qiuxia
Published: November 2022

Cyclic N-halamines are highly antimicrobial, very stable, and not susceptible to bacterial resistance. A polysiloxane delivery vehicle was synthesized to deliver cyclic imide N-halamine onto cellulose via a benign and universal procedure that does not require a harmful solvent or chemical bonding. In brief, Knoevenagel condensation between barbituric acid and 4-hydroxybenzaldehyde furnished 5-(4-hydroxybenzylidene)pyrimidine-2,4,6-trione, whose phenolic O−H was subsequently reacted with the Si−H of poly(methylhydrosiloxane) (PMHS) via silane alcoholysis. The product of silane alcoholysis was interpenetrated into cellulose in supercritical CO2 (scCO2) at 50 °C, to form a continuous modification layer. The thickness of the modification layer positively correlated with interpenetration pressure in the experimental range of 10 to 28 MPa and reached a maximum value of 76.5 nm, which demonstrates the ability for tunable delivery, to control the loading of the imide N−H bond originating from barbituric acid unit. The imide N−H bonds on cellulose with the thickest modifier were then chlorinated into N−Cl counterparts using tert-butyl hypochlorite, to exert a powerful biocidability, providing ~7 log reductions of both S. aureus and E. coli in 20 min. The stability and rechargeability of the biocidability were both very promising, suggesting that the polysiloxane modifier has a satisfactory chemical structure and interlocks firmly with cellulose via scCO2 interpenetration.
Computational Micromechanics Investigation of Percolation and Effective Electro-Mechanical Properties of Carbon Nanotube/Polymer Nanocomposites using Stochastically Generated Realizations: Effects of Orientation and Waviness
Authors: Talamadupula Krishna Kiran , Seidel Gary
Published: November 2022

The electrical and mechanical properties of carbon nanotube/polymer nanocomposites depend strongly upon several factors such as CNT volume fraction, CNT alignment, CNT dispersion and CNT waviness among others. This work focuses on obtaining estimates and distribution for the effective electrical conductivity, elastic constants and piezoresistive properties as a function of these factors using a stochastic approach with numerous CNT/polymer realizations coupled with parallel computation. Additionally, electrical percolation volume fraction and percolation transitional behavior is also studied. The effective estimates and percolation values were found to be in good agreement with experimental works in the literature. It was found that with increasing CNT volume fraction, the mechanical properties improved. However, due to the interaction of CNTs with one another through electrical tunneling, the conductivity and piezoresistivity properties evolved in a more complex manner. While the degree of alignment played a strong role in the effective properties making them anisotropic, the effect of waviness was found to be insubstantial.
Rational Design of Solid Polymer Electrolyte Based on Ionic Liquid Monomer for Supercapacitor Applications via Molecular Dynamics Study
Authors: Demir Baris , Chan Kit-Ying , Livi Sébastien
Published: November 2022

The safety concern arising from flammable liquid electrolytes used in batteries and supercapacitors drives technological advances in solid polymer electrolytes (SPEs) in which flammable organic solvents are absent. However, there is always a trade-off between the ionic conductivity and mechanical properties of SPEs due to the lack of interaction between the ionic liquid and polymer resin. The inadequate understanding of SPEs also limits their future exploitation and applications. Herein, we provide a complete approach to develop a new SPE, consisting of a cation (monomer), anion and hardener from ions–monomers using molecular dynamics (MD) simulations. The results show that the strong solid–liquid interactions between the SPE and graphene electrode lead to a very small gap of ∼5.5 Å between the components of SPE and electrode, resulting in a structured solid-to-liquid interface, which can potentially improve energy storage performance. The results also indicated the critical role of the mobility of free-standing anions in the SPE network to achieve high ionic conductivity for applications requiring fast charge/discharge. In addition, the formations of hardener-depleted regions and cation–anion-poor/rich regions near the uncharged/charged electrode surfaces were observed at the molecular level, providing insights for rationally designing the SPEs to overcome the boundaries for further breakthroughs in energy storage technology.
From the Neue Gemeinschaft to Bar Kochba: The Jewish Communitas or the Idea of Jewish Politics as Mysticism
Authors: Engel Amir
Published: November 2022

This essay uses both published and archival material to reconstruct the ideological and social contexts of Martin Buber’s 1909 address “Judaism and the Jews”. It suggests that Buber’s address became immensely influential because it equated mysticism and politics into one metaphor. Secondly, it shows that Buber imported this idea from debates and discussions that took place almost a decade earlier in Berlin, among the bohemian circle known as the Neue Gemeinschaft (new community). Finally, the author hopes to show that the social context can be as crucial to understanding an idea as the ideological context. The question about the functions and potentials of the “community” so central to Buber and the Neue Gemeinschaft must be examined, this essay contends, not only conceptually but also as a lived reality. In order to get a glimpse of “the new community”, this essay reproduces archival material that testifies not only to what people thought but also what they did, who they were, and how they interacted.
Assessment of Intestinal Ischemia–Reperfusion Injury Using Diffuse Reflectance VIS-NIR Spectroscopy and Histology
Authors: Hou Jie , Ness Siri Schøne , Tschudi Jon , O’Farrell Marion , Veddegjerde Rune , Martinsen Ørjan Grøttem , Tønnessen Tor Inge , Strand-Amundsen Runar
Published: November 2022

A porcine model was used to investigate the feasibility of using VIS-NIR spectroscopy to differentiate between degrees of ischemia–reperfusion injury in the small intestine. Ten pigs were used in this study and four segments were created in the small intestine of each pig: (1) control, (2) full arterial and venous mesenteric occlusion for 8 h, (3) arterial and venous mesenteric occlusion for 2 h followed by reperfusion for 6 h, and (4) arterial and venous mesenteric occlusion for 4 h followed by reperfusion for 4 h. Two models were built using partial least square discriminant analysis. The first model was able to differentiate between the control, ischemic, and reperfused intestinal segments with an average accuracy of 99.2% with 10-fold cross-validation, and the second model was able to discriminate between the viable versus non-viable intestinal segments with an average accuracy of 96.0% using 10-fold cross-validation. Moreover, histopathology was used to investigate the borderline between viable and non-viable intestinal segments. The VIS-NIR spectroscopy method together with a PLS-DA model showed promising results and appears to be well-suited as a potentially real-time intraoperative method for assessing intestinal ischemia–reperfusion injury, due to its easy-to-use and non-invasive nature.
A Novel Cost-Effective Nanobody against Fumonisin B1 Contaminations: Efficacy Test in Dairy Milk and Chickens
Authors: Chen Yi , Qu Guanggang , Quan Hongkun , Wang Yihui , Wang Changjiang , Haque Md Atiqul , He Cheng
Published: November 2022

Background: Fumonisin B1 (FB1) is a secondary metabolite produced mainly by Fusarium verticillioides or Fusarium proliferatum. It poses a huge threat to the sustainable animal industry and human health as well via food chains (egg, meat and milk). Although E. coli-expressed nanobodies are documented for diagnostic applications, nanobodies remain elusive as FB1 detoxifiers in feed and food. Results: In the present study, the E. coli-expressed nanobody was assessed to remove FB1 in fresh milk, embryonated eggs and broilers. Firstly, 2 alpacas received intramuscularly FB1-adjuvanted BSA 6 times, and then the variable domain of the heavy-chain antibody (VHH) of fb1 genes were amplified to clone into the pCANTAB 5 E vector in order to generate a VHH-FB1 phage antibody display library, yielding 3.4 × 1010 capacity with 96.7% positivity. Afterwards, 5 anti-FB1 nanobodies were expressed and identified. Furthermore, maximal 43.2% FB1 was removed from milk by 1:2000 concentration of nanobody 5 (Nb5). Furthermore, SPF-embryonated eggs were inoculated into albumens with nanobody-treated FB1. The Nb5 group yielded an 83.3% hatching rate, higher body weight, lower gizzard ulceration and fewer FB1 residuals. In order to warrant the above results, 50 broilers aged 10 days were received orally with 20 ppm of FB1 for 20 days. At the same time, birds were fed orally with 50 μg of Nb5 or bivalent nanobody 11 (BiNb11). Finally, the Nb5 group showed a higher relative body weight gain and lower gastric ulcerations and fewer inflammations in the thymus and bursa. Conclusions: Based on the above evidence, the Nb5 nanobody may be considered as an additional FB1 detoxifier, contributing to FB1 decontamination.