bluuebunny/crossref_metadata_2025 huggingface dataset:
Studying a poxvirus gene capture model through recombination and reactivation
Authors: Johnson Katherine T. , Evans Dr. David H.
Published: July 2022

Introduction: Vaccinia poxvirus (VACV) is a double stranded DNA virus that replicates in the cytoplasm of infected cells. Some VACV genes resemble homologs of host genes and appear to have been captured from the cell; however, since poxviruses are confined to the cytoplasm, researchers are unclear as to how these viruses acquire this homology (1). If a cellular mRNA was accidentally reverse transcribed into cDNA, which could occur during retrovirus co-infection, a poxvirus might be able to incorporate this sequence into its own genome through rare non-homologous (homology-independent) recombination.

Methods: We modeled this process using two different recombination systems and substituted a DNA encoding mycophenolic acid (MPA), a selectable marker, for the hypothetical non-homologous host cDNA. We prepared DNA constructs containing this marker along with 20 base pairs homologous to the 5’ and 3’ flanking regions of the VACV-encoded NotI restriction site. A construct without this flanking homology was also prepared. The “passive” recombination system used a helper poxvirus to reactivate VACV DNA; in contrast, VACV infected BSC40 cells were transfected with the construct in the “active” recombination system.

Results: The “passive” recombination system generated 105 PFU/mL of reactivated VACV; however, no recombinants containing the selectable marker were detected. The “active recombination” method generated 106 PFU/mL of total VACV and approximately 10 PFU/mL of recombinant virus for both homology containing and non-homology containing constructs.

Discussions: We were unable to determine the recombination frequency of the “passive system” because recombinant virus was not detected. Based on virus titers determined from plaque assays, we approximated the recombination frequency of the “active system” to be ≤ 10-5. We are currently cloning and sequencing viruses resulting from non-homologous recombination to determine where the MPA marker is located. Preliminary analysis of these types of clones (data not shown in this paper) suggests that the transfected DNAs are being incorporated into a diversity of sites, some located near the boundary of the VACV genome where the right terminal inverted repeat begins. In summary, our findings suggest that the recombination frequencies in both methods are very low and better methods of selection are needed to observe these rare events. Future studies of recombinant clones are needed to gain a better understanding of this non- homologous gene capture process.
The effect of Eurosta solidaginis parasitism on pollinator preference in Solidago canadensis
Authors: Meyer Adam , Salter Natasha , Shooner Stephanie
Published: July 2022

Solidago canadensis is a self-incompatible perennial species indigenous to North America that reproduces asexually via rhizomes and sexually via seeds. It is the favoured host of the gall fly, Eurosta solidaginis. Sexual reproduction leads to faster rates of adaptation in stressful environments and may be advantageous in the maintenance of host-parasite coevolution. The effect of infection by the gall fly on pollinator visitations at the patch and at the ramet level was assessed as a proxy for the ability to sexually reproduce. The study was conducted by analyzing pollinator preference at both the patch level and individual ramet level through successive observations of pollinator visitations. Though the variation in the number of pollinator visitations could be accounted for by time of day and median bloom stage, the percentage of infected ramets in a patch was not a significant explanatory variable. This suggests that gall formation does not affect pollinator preference and that the capacity to sexually reproduce is likely not reduced because of the host-parasite interaction. Broadly, this study served as an example of how pollinator preference may be utilized as a measure of fitness, and to further understand how selective pressures affect plant populations that reproduce both sexually and asexually.
Parrotfish Body Size As An Indicator of Diurnal Fish Species Richness On Fringing Coral Reefs in Barbados
Authors: Eger Aaron , Pigeon-Dubeau Catherine , Sibileau Lauriane
Published: July 2022

Background: Coral reefs around the world are host to some of the most condensed and varied ecosystems. However, over the past decades, their biodiversity has alarmingly decreased. A rapid and reliable way of assessing their status is urgently needed to monitor and prevent their decline. The purpose of this study is to assess whether or not family Scaridae (common name: parrotfish) body size can be used as an index to evaluate the diurnal fish diversity on coral reefs.

Methods: We selected 6 accessible reefs on the West coast of Barbados and measured the size of parrotfish we encountered, as well as the number of fish species present on the reef; this data was then plotted and statistically analyzed to establish a possible correlation.

Results: Our results show that reef fish species richness is strongly correlated to both the ratio of large to small parrotfish and the average parrotfish size. It is however the large to small ratio that exhibited the strongest relationship. Further analysis revealed that the population size of large parrotfish correlates with reef biodiversity with a Pearson’s r coefficient of 0.97.

Conclusion: This relationship could be due to the fact that large parrotfish (greater than or equal to 20 cm) have increased grazing rates compared to smaller ones, this increased grazing promotes coral polyp recruitment, thereby benefiting the diversity observed on coral reefs. Further research is needed to elucidate whether these results can be extended to other areas of the Caribbean and provide conservation efforts with an easy tool to survey and protect coral reef ecosystems.
High UV Excitation Intensity Induces Photoconversion of DAPI During Wide-Field Microscopy
Authors: Rodic Stefan , Brown Claire , Wee Erika (Tse-Luen)
Published: July 2022

Background: Multi-color fluorescence microscopy is dependent on the spectral specificity of the dyes and probes used for localization. One of the most commonly used fluorescent DNA dyes is DAPI, which is usually excited by UV light to emit in the blue visible light range. Herein, we describe a pattern of decreasing DAPI fluorescence upon extended UV exposure, closely followed by an increase in emission maxima in the green range.

Methods: UV-induced photo-conversion of DAPI to green-fluorescing photoproducts was studied on Chinese hamster ovary cells using wide-field fluorescence microscopy, at different UV exposure times and intensities. Imaging was done in repetitive cycles, by alternating between a DAPI and FITC filter cube and following this with a 1 second UV excitation time. The effect of differing UV light intensities on the photo- conversion process was not previously described in the literature.

Results: Upon image analysis from a large sample of cells, the rate of photo-conversion was shown to be dependent on both the duration of UV excitation and the intensity of the UV light source. Both the process of DAPI depletion and photo-product growth showed biphasic exponential patterns of change. Furthermore, the level of DAPI fluorescence intensity was found to be negatively correlated with the green fluorescence of the photo-product.

Limitations: This study did not examine the effect of differing mounting media or a variation in DAPI concentration on the rate of DAPI photo-conversion. Also, the exact light dosage to the system was not measured from the 100W Hg bulb. Photo-bleaching of green fluorescence in cells not stained with DAPI was not measured to control for bleaching of endogenous cell molecules.

Conclusion: Based on our findings, a set of recommendations was formulated in order to help reduce the effects of UV-induced DAPI photo-conversion.
Tiempo de preguntas. Retos de la democracia latinamericana en la década en curso
Authors: Ruiz Méndez Alberto
Published: July 2022

El objetivo de este trabajo, desplegado a través de una serie de preguntas, es trazar un mapa de ruta sobre los problemas que enfrentarán las democracias latinoamericanas durante la década en curso y algunas estrategias para enfrentarlos. En un primer momento, se describe el estado de debilidad actual de la democracia en el mundo y en la región, a partir de los datos publicados en diversos informes a finales del 2021; enseguida, con base en aquellos datos, se propondrá al populismo institucionalizado y la polarización afectiva como los malestares causantes de su debilidad; finalmente, se presenta a la democracia como un sistema ascendente para la toma de decisiones colectivas que podría servir como marco de comprensión y análisis de aquellos dos malestares.
Dimerization of kringle 1 domain from hepatocyte growth factor/scatter factor provides a potent MET receptor agonist
Authors: de Nola Giovanni , Leclercq Bérénice , Mougel Alexandra , Taront Solenne , Simonneau Claire , Forneris Federico , Adriaenssens Eric , Drobecq Hervé , Iamele Luisa , Dubuquoy Laurent , Melnyk Oleg , Gherardi Ermanno , de Jonge Hugo , Vicogne Jérôme
Published: July 2022

Hepatocyte growth factor/scatter factor (HGF/SF) and its cognate receptor MET play several essential roles in embryogenesis and regeneration in postnatal life of epithelial organs such as the liver, kidney, lung, and pancreas, prompting a strong interest in harnessing HGF/SF-MET signalling for regeneration of epithelial organs after acute or chronic damage. The limited stability and tissue diffusion of native HGF/SF, however, which reflect the tightly controlled, local mechanism of action of the morphogen, have led to a major search of HGF/SF mimics for therapy. In this work, we describe the rational design, production, and characterization of K1K1, a novel minimal MET agonist consisting of two copies of the kringle 1 domain of HGF/SF in tandem orientation. K1K1 is highly stable and displays biological activities equivalent or superior to native HGF/SF in a variety of in vitro assay systems and in a mouse model of liver disease. These data suggest that this engineered ligand may find wide applications in acute and chronic diseases of the liver and other epithelial organs dependent of MET activation.
O vestuário na História do Cinema e a sua receção crítica
Authors: Cucinotta Caterina , Pereira Ana Catarina
Published: July 2022

A presença icónica do vestuário no cinema perpassa toda a História da sétima arte, dos primeiros filmes aos contemporâneos, sendo geradora de tendências, géneros, estereótipos, sucessos e fracassos de bilheteira, aplausos ou desagrados da crítica especializada. O dossiê que aqui iniciamos parte deste princípio, sendo dedicado à importância e à visibilidade do figurino do cinema, e relacionando, de uma perspectiva teórica, os estudos de género e as materialidades fílmicas.[...]
Productivity of fodder agrocenoses in the Northern territories of the Arkhangelsk region
Authors: Shamanin A. A. , Popova L. A.
Published: July 2022

The studies were conducted in 2019-2021 in the conditions of the northern part of the Arkhangelsk region. The aim of the research is to study perennial agrophytocenoses consisting of promising cereals and legumes to improve the fodder base in the conditions of northern regions. Herbage grasses of 3 years of life formed from various combinations of cereals and legumes were studied, their productivity and participation of components of grass mixtures in the formation of yields were determined. Meteorological conditions varied during the years of the study. In 2019 and 2020, the growing seasons were excessively wet (hydrothermal coefficient (HTC) 1.80 and 1.76), in 2021, the period was noted as quite wet (HTC 1.38). It was found that in the 1st year of life the formation of green mass yield is due to the legume component. At the same time, 73% of red clover and 59-67% of alfalfa are contained in the structure of the crop. Later in the phytocenosis there is a decrease in the participation of leguminous grasses in the yield, in the 3rd year the content of red clover was 15- 20%, alfalfa fell out of the herbage. The herbage, consisting of reed fescue and red clover, stood out as the most productive. Two-cuts use of this agrophytocenosis allows for the first 2 years of intensive use to get from 10.71 to 11.41 t/ha of dry matter, 114.12-129.01 GJ/ha of exchange energy and 1.43- 1.51 t/ha of crude protein. Reed fescue stands out as the most promising crop. When alfalfa falls out of the herbage, the experiment variant with reed fescue is superior to the control variant in dry matter yield and exchange energy yield.
Relationships between Smoking, Age, Body Mass Index, and Abstinence with DNA Fragmentation Index in Male Infertile Patients
Authors: Ediningtyas Atifa Nadira , Laqif Abdurahman , Melinawati Eriana , Respati Supriyadi Hari
Published: July 2022

Background: Low birth weight (LBW) is defined as a newborn baby weighing less than 2500 grams. Low birth weight is a serious condition in developing countries. The impact of cigarette smoke on pregnant women who are exposed to cigarette smoke is at a higher risk of complications, such as miscarriage, premature birth, or babies born with low weight. This study aims to analyze the relationship between exposure to secondhand smoke and low birth weight.
Subjects and Method: This research is a systematic review and meta-analysis by systematically reviewing primary articles from Pubmed, Google Scholar, Scient Direct and Springerlink by selecting articles published in 2012-2022. The articles searched using PICO were P: Pregnant women, I: exposed to cigarette smoke, C: not exposed to cigarette smoke, O: low birth weight. Keywords "Exposure to smoking pregnant women" OR "Expectant mother" OR "Pregnancy women" OR "Exposed to cigarettes" OR "Secondhand smoke exposure" OR "Cigarette smoke expo- sure" AND "Low birth weight" OR "Low weight babies" AND “Case control” AND “Adjusted odds ratio”. Articles used PRISMA flow diagrams and analyzed using Review Manager 5.3.
Results: There were nine articles analyzed consisting of Nepal, Morocco, Ethiopia, Sierra Leone, India, Indonesia and China. Meta-analysis showed that cigarette smoke exposure increased the risk of low birth weight and was statistically significant (aOR = 2.19; 95% CI = 1.58 to 3.03; p < 0.001). Conclusion: Exposure to cigarette smoke increases the risk of weight gain in LBW infants by 2.19 times compared to not exposed to cigarette smoke.
Keywords: exposure to cigarette smoke, low birth weight, meta-analysis.
Correspondence:Winei Handriani. Master’s Program in Public Health, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Surakarta 57126, Jawa Tengah. Email: wineihandriani02@gmail.com. Mobile: 083150000253.
Correlation between Passive Cigarette Smoke Exposure on Low Birth Weight: Meta-Analysis
Authors: Handriani Winei , Budihastuti Uki Retno , Pamungkasari Eti Poncorini
Published: July 2022

Background: Low birth weight (LBW) is defined as a newborn baby weighing less than 2500 grams. Low birth weight is a serious condition in developing countries. The impact of cigarette smoke on pregnant women who are exposed to cigarette smoke is at a higher risk of complications, such as miscarriage, premature birth, or babies born with low weight. This study aims to analyze the relationship between exposure to secondhand smoke and low birth weight.
Subjects and Method: This research is a systematic review and meta-analysis by systematically reviewing primary articles from Pubmed, Google Scholar, Scient Direct and Springerlink by selecting articles published in 2012-2022. The articles searched using PICO were P: Pregnant women, I: exposed to cigarette smoke, C: not exposed to cigarette smoke, O: low birth weight. Keywords "Exposure to smoking pregnant women" OR "Expectant mother" OR "Pregnancy women" OR "Exposed to cigarettes" OR "Secondhand smoke exposure" OR "Cigarette smoke expo- sure" AND "Low birth weight" OR "Low weight babies" AND “Case control” AND “Adjusted odds ratio”. Articles used PRISMA flow diagrams and analyzed using Review Manager 5.3.
Results: There were nine articles analyzed consisting of Nepal, Morocco, Ethiopia, Sierra Leone, India, Indonesia and China. Meta-analysis showed that cigarette smoke exposure increased the risk of low birth weight and was statistically significant (aOR = 2.19; 95% CI = 1.58 to 3.03; p < 0.001). Conclusion: Exposure to cigarette smoke increases the risk of weight gain in LBW infants by 2.19 times compared to not exposed to cigarette smoke.
Keywords: exposure to cigarette smoke, low birth weight, meta-analysis.
Correspondence: Winei Handriani. Master’s Program in Public Health, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Surakarta 57126, Jawa Tengah. Email: wineihandriani02@gmail.com. Mobile: 083150000253.
Meta-Analysis the Effect of Prenatal Yoga to Lower Anxiety and Stress in Intrapartum Women
Authors: Wulandari Adetya , Prasetya Hanung , Murti Bhisma
Published: July 2022

Background: Pregnant women are very susceptible to complications or high risk during pregnancy, especially before delivery, causing anxiety and stress. Prenatal yoga is known to relax breathing which can relax muscles and reduce anxiety. The purpose of this study was to analyze the effect of prenatal yoga on pregnant women on anxiety and stress in the face of labor.
Subjects and Method: This study was a meta-analysis with the following PICO, population: pregnant women. Intervention: prenatal yoga. Comparison: not doing prenatal yoga. Result: reduced stress and anxiety. Keywords to search for articles “Prenatal Yoga” OR “Pregnancy yoga” AND “Anxiety” AND “Stress” AND “randomized controlled trial”. The articles included are full-text English and a randomized controlled trial study design from 2009-2021. Article selection is done by using PRISMA flow diagram. Articles were analyzed using the Review Manager 5.3 application.
Results: A total of 12 RCT studies from the Asian, American and European continents were selected for systematic review and meta-analysis. Based on 9 articles stated that pregnant women who do prenatal yoga can reduce anxiety about childbirth by 0.86 times compared to not doing prenatal yoga (SMD = -0.86; 95% CI -1.50 to -0.21; p= 0.010) and 5 articles state that pregnant women those who do prenatal yoga can reduce stress during childbirth by 1.23 times compared to those who do not do prenatal yoga (SMD= -1.23; 95% CI= -1.59 to -0.87; p< 0.001).
Conclusion: Prenatal yoga can reduce anxiety and stress in pregnant women in the face of childbirth.
Keywords: anxiety, stress, pregnancy, prenatal yoga
Correspondence: Adetya Wulandari. Master’s Program in Public Health, Universitas Sebelas Maret. Jl. Ir. Sutami 36A, Surakarta 57126, Central Java, Indonesia. Mobile: 081274814357. Email: Adetya1507@gmail.com.
Flora útil de Hidalgo
Authors: Pérez Escandón Blanca Estela , López Gutiérrez Berenice Nathaly , Reyes Silva Jesús Alan , Villavicencio Nieto Miguel Ángel
Published: July 2022

El estudio describe el manejo y la riqueza de especies que se encuentran en los huertos familiares del municipio de Zapotlán de Juárez, en el estado de Hidalgo y poca utilización de insumos.
Determination of the Antifungal Effect of Bacterial Metabolites of Xenorhabdus szentirmaii Against Some Phytopathogenic Fungi
Authors: ALTIN Nedim , GÜLCÜ Barış
Published: July 2022

This study was carried out to determine the antifungal effects of the supernatant produced by the bacterium Xenorhabdus szentirmaii, which is associated with soil-inhabiting entomopathogenic nematodes, on important plant pathogenic fungi, Fusarium verticilliodes, Fusarium oxysporum f.sp lycopersici, Fusarium oxysporum f.sp radicis lycopersici, Botrytis cinerea, Sclerotinia sclerotiorum and Phytophthora nicotianae. The effects of 1, 3, 5 and 7% concentrations of the supernatant produced by X. szentirmaii bacteria on mycelium growth of the fungal pathogens was determined at 3, 7 and 14 days after application (dap). The effects of the supernatant on the formation of sporangia and the emergence of zoospores from the sporangium of Phytophthora nicotianae was also determined. According to the results of the study, the highest dose of the supernatant (7% concentration) prevented mycelium development of all tested pathogens. The effect of the supernatant decreased at 14 dap against F. verticilliodes, F. oxysporum f.sp lycopersici, F. oxysporum f.sp radicis lycopersici. The antifungal effect on S. sclerotiorum completely disappeared in 14 days whereas the effect continued even in 14 days against B. cinerea and P. nicotianae. Xenorhabdus szentirmaii supernatant at a concentration of 7% was highly efficacious on the formation of sporangia and the emergence of zoospores within the sporangium of P. nicotianae at a concentration of 7%.
Effects of TiN/CrN, CrAlN, and TiN Coatings on the Performance of AISI M2 Tool Steel
Authors: KAYIŞ Cemile , DİLER Ege Anıl , SANDALLI Hatice , AĞARER Fuat Can
Published: July 2022

The aim of this study is to investigate the effects of various ceramic-based coatings on the performance of AISI M2 high-speed steel punch used in the nut piercing process. AISI M2 punches were coated with TiN, TiN/CrN, and CrAlN utilizing a physical vapour deposition method. The total stroke number of the punches was used to evaluate the tool life of TiN, CrAlN, and TiN/CrN coated AISI M2 punches. AISI M2 punch coated with TiN/CrN was able to work up to greater stroke numbers than punches coated with TiN and CrAlN. Hardness and wear tests were performed to study the wear resistance of TiN/CrN and CrAlN coatings under relatively normal load conditions compared to actual working conditions (impact loading conditions) in the piercing process. The hardness and wear resistance of the CrAlN coating were greater than those of the TiN/CrN coating. The wear performance of the CrAlN coating was higher at normal loads and sliding speeds while the tool life of the TiN/CrN-coated punch was better under impact loading conditions.
Investigation of activity of Tobamovirus in pepper plants containing L4 resistance gene
Authors: FİDAN Hakan , GONBADİ Ailar , SARIKAYA Pelin , ÇALIŞ Özer
Published: July 2022

Pepper mild mottle virus (PMMoV) is a plant virus belonging to the Virgaviridae family; it significantly reduces pepper yield production worldwide. The PMMoV is spread by contaminated seeds and there is no chemical treatment available. Therefore, resistant pepper varieties containing the L4 gene are recommended for the management of PMMoV. A considerable amount of evidence suggests that the L4 gene confers resistance to PMMoV in pepper. The aim of the project is to confirm the status of the L4 gene for resistance to PMMoV in pepper varieties, several inoculations were performed on pepper plants containing L3, L4 resistant genes and susceptible pepper plants without the resistance genes. The L4 resistant plants produced mottling, mosaic, leaf curl, stem necrosis symptoms in the tested pepper plants but there was no amplicon observed with specific primers of PMMoV in RT-PCR analyses. To determine if the L3 and L4 genes are controlling resistance to PMMoV, RT-PCR analyzes were conducted using PMMoV and Tomato brown rugose fruit virus (ToBRFV) where both viruses belong to the same family. The molecular studies revealed that the L4 gene controls resistance mechanisms to PMMoV but it is not able to govern Tobamovirus, ToBRFV. We showed that pepper plants harboring the L3 and L4 gene have the ability to precisely control the mechanism of resistance to PMMoV compared to pepper plants carrying only the L3 gene. A complete genome sequence of PMMoV was obtained and submitted to Genbank with MW523006 accessıon number in the NCBI system.
Determination of endogenous zeatin/IAA levels in selected fortune mandarin mutants against Alternaria alternata pv. citri
Authors: TURGUTOĞLU Ertuğrul , BAKTIR İbrahim
Published: July 2022

Alternaria brown spot is a serious disease in mandarins and mandarin hybrids. In this particular research, 9 Alterneria tolerant and 2 susceptible mutant individuals obtained from a series of in vitro and in vivo studies were used. Endogenous indole acetic acid (IAA) and zeatin hormone levels of the individuals were noted before and after the Alternaria disease inoculations in order to determine the hormonal variations during the research. It was determined that endogenous zeatin levels decreased after the inoculation compared to its initial levels. The indole acetic acid levels of the individuals increased after inoculation except for some individuals.
Occupational Disappointment and Emergency Nurses: A Qualitative Descriptive Study
Authors: Zullo Jiun-yi , Corcoran Lynn , Cook Karen
Published: July 2022

Background: Occupational disappointment is a novel concept in emergency nursing. It is a feeling of disheartenment with career choice.  It results from prevalent, unaddressed verbal abuse in the emergency department directed towards nurses from patients and/or their visitors. Occupational disappointment is conceptually different from burnout and compassion fatigue. In the context of the COVID-19 pandemic, it is important to acknowledge this phenomenon and understand its implications while considering strategies to mitigate it.

Method: A qualitative descriptive methodology was used in this study. Nurses were interviewed to explore the question: How do emergency department nurses experience occupational disappointment as a result of verbal abuse?

Results: Three major themes with several subthemes were identified: (1) nurses’ experiences of occupational disappointment with sub-themes of powerlessness and normalizing; (2) nurses’ responses to occupational disappointment with sub-themes of changes in nursing practice, retention, and nurses’ mental health; (3) nurses’ concerns regarding occupational disappointment with sub-themes of nursing and organizational leadership.

Discussion: Policies addressing verbal abuse would help guide nurses when managing this violence. Failure of nurse leaders to implement such measures contributes to nurses’ occupational disappointment, consequently affecting nurses’ practice, mental health, and retention. While these implications are not new, the COVID-19 pandemic has exacerbated this phenomenon. The magnitude of verbal abuse that emergency nurses currently face has increased exponentially; a renewed urgency for strategic action is necessary.

Conclusion: Occupational disappointment is a direct result of verbal abuse and an indirect result of organizational failures to support nurses and empower them to mitigate this abuse.
Saberes populares e alfabetização científicas: possibilidades de um curso a distância na formação continuada de professores de ciências
Authors: Aparecida Rosendo da Silva Larissa , Milaré Tathiane
Published: July 2022

Este trabalho discute a essencialidade da formação continuada na carreira docente, assim como as possibilidades e contribuições de um curso de formação continuada de professores sobre saberes populares e Alfabetização Científica e Tecnológica, no contexto da extensão universitária e na modalidade a distância, para o desenvolvimento profissional de professores de Ciências da Natureza. O arcabouço teórico foi construído a partir dos aspectos políticos e históricos que alicerçam o uso da Educação a Distância e os apoios teóricos  da estrutura do curso ofertado. Concluiu-se que o curso desenvolvido contemplou os pressupostos para um momento formativo adequado para os professores em atuação, assim como os aspectos necessários a uma formação orientada para a Alfabetização Científica e Tecnológica, proporcionando uma formação centrada na valorização cultural e a multiculturalidade em sua articulação com sabedoria popular.
A utilização da plataforma Kahoot como ferramenta de gamificação: uma contribuição para o ensino e a aprendizagem na educação básica
Authors: Maciel da Silva Andréia , Priscila Costa Ferreira Débora
Published: July 2022

Existem diversas concepções voltadas ao ensino e aprendizagem, muitas delas tentando explicar os desafios dessa relação, dentre os quais está a falta de interesse dos alunos, cada vez mais antenados com as novas tecnologias. Como forma de reverter estas e outras problemáticas, passou-se a discutir cada vez mais a adoção de metodologias ativas e atrativas de ensino, como a gamificação. Este artigo tem como objetivo reconhecer o uso da gamificação no ambiente educacional e como pode ser aplicada por meio da plataforma Kahoot!, de forma a contribuir com o ensino e aprendizagem dos componentes curriculares da educação básica. Para tanto, será realizada uma revisão bibliográfica acerca do tema, assim como um estudo exploratório do ambiente virtual aqui evidenciado. Por meio dos resultados, é possível reconhecer o uso da gamificação no ambiente educacional, através da plataforma Kahoot!, à medida que mais trabalhos científicos vêm sendo divulgados acerca dessa temática. Enfim, a plataforma contribui com o ensino e aprendizagem por meio da abordagem de conteúdos de forma mais atrativa, proporcionando experiências cognitivas, sociais e motoras, a autoavaliação e intervenção pedagógica mais efetiva.
Bullying e cyberbullying: um estudo em tempos de pandemia
Authors: Gonçalves Vitor , Emanuela Augusto Vaz Cátia
Published: July 2022

O objetivo deste estudo é averiguar se a situação pandémica agravou ou não as situações de bullying escolar. A metodologia utilizada foi o estudo de caso, de carácter exploratório e descritivo, onde se articularam os paradigmas quantitativo e qualitativo. Participaram neste estudo 370 alunos do 2º Ciclo do Ensino Básico (5º e 6º anos) do ano letivo 2020/2021, de dois agrupamentos de escolas do concelho de Bragança, no nordeste transmontano de Portugal continental. O instrumento de recolha de dados utilizado foi um inquérito por questionário ou survey construído no Google Forms e disponibilizado online na segunda quinzena de maio de 2021. Após a recolha e análise dos resultados verificou-se que, durante a pandemia, as situações de bullying diminuíram, embora o cyberbullying tenha aumentado ligeiramente, verificou-se uma diminuição das vítimas e correspondente diminuição das testemunhas que alteraram a sua forma de atuação perante este problema. Os resultados obtidos neste estudo servirão, após o alívio de muitas das medidas restritivas, como alavanca para incentivar as instituições educativas envolvidas neste estudo a olhar este problema como uma situação que exige a tomada de medidas para a sua minimização, para além de complementar outros estudos no contexto português ou mesmo na Península Ibérica.
Development of problem-solving based test instruments to foster the students creative thinking skills on environmental conservation
Authors: Rizkia Annissa Dwi , Sjaifuddin Sjaifuddin , Suryani Dwi Indah
Published: July 2022

This study aims to obtain data on the feasibility of problem-solving-based test instruments to foster the creative thinking skills of junior high school students on environmental conservation. The method used in this study is Research & Development (R&D) with development procedures according to Sugiyono. This study was conducted in 5 stages: potentials and problems, data collection, product design, design validation, and design revision. The instrument used in this development research is a question validation sheet of material experts, evaluation experts, and expert practitioners. The results showed that based on the assessments of content experts, evaluation experts, and practitioners, scores of 88.4%, 88.6%, and 89.9% were obtained, which were classified in the "Very Feasible" category, respectively. In conclusion, the overall validation of the problem-solving-based test instrument received a score of 88.9%, which belongs to the "Very Feasible" category, so the test instrument can be used as an alternative tool for evaluating science learning for junior high school students with several revisions.
Amenities and the attractiveness of New Zealand cities
Authors: Preston Kate , Maré David C , Grimes Arthur , Donovan Stuart
Published: July 2022

We analyse which factors attract people and firms (and hence jobs) to different settlements across New Zealand. Using theoretically consistent measures derived within the urban economics literature, we compile quality of life and quality of business indicators for 130 ‘cities’ (settlements) from 1976 to 2013, using census rent and wage data. Our analyses both include and exclude the three largest cities (Auckland, Wellington, Christchurch). Places that are attractive to live in tend to be sunny, dry and near water (i.e. the sea or a lake). Since the mid1990s, attractive places have also had relatively high shares of the workforce engaged in education and (to a lesser extent) health. Attractive places have high employment shares in the food, accommodation, arts and recreation service sectors; however (unlike for education and health) we find no evidence that quality of life is related to changes in employment share for these sectors. The quality of business is highest in larger cities, and this relationship is especially strong when the country’s three largest cities are included in the analysis.
Review on Applications of R programming in Biological Data Analysis
Authors:   Department of Plant Molecular Biology and Bioinformatics Centre for Plant Molecular Biology and Biotechnology Tamil Nadu Agricultural University, Coimbatore -3, S Selva Babu , A Kiruthika ,   Department of Plant Molecular Biology and Bioinformatics Centre for Plant Molecular Biology and Biotechnology Tamil Nadu Agricultural University, Coimbatore -3, V Ragapriya ,   Department of Plant Molecular Biology and Bioinformatics Centre for Plant Molecular Biology and Biotechnology Tamil Nadu Agricultural University, Coimbatore -3, V Anandhi ,   Department of Physical Sciences & Information Technology, AEC&RI, TNAU, Coimbatore-641003, N Saranya ,   Department of Plant Molecular Biology and Bioinformatics Centre for Plant Molecular Biology and Biotechnology Tamil Nadu Agricultural University, Coimbatore -3
Published: July 2022

The key programming language used in the field of data science is R programming, because of the freeavailability and ability to handle huge data. Packages developed in the R are used widely by scientists toobtain solutions for various real-world problems in several disciplines like healthcare, agriculture, andinformation technology. Next-generation sequencing data requires various bioinformatics analyses likequality checking, differential gene expression studies, and annotation which is facilitated by R packages andsoftware. Bioconductorprojectremains a hub of R packages that are open access and move the researchers tothe comfort zone for various kinds of analysis. In this review, we briefly discussed various packages usefulfor NGS data analysis and also explained how to use the packages for basic level analysis that benefitsresearchers having less exposure to R programming.
Awareness and Adoption Behavior of Vegetable Farmers on Safe Pesticide Handling Practices
Authors:   Department of Agricultural Extension and Rural Sociology, Agricultural College and Research Institute, TNAU, Madurai., M Rakesh , R Velusamy ,   Department of Agricultural Extension and Rural Sociology, Agricultural College and Research Institute, TNAU, Madurai., K Ramakrishnan ,   Department of Agricultural Extension and Rural Sociology, Agricultural College and Research Institute, TNAU, Madurai., M Radha ,   Department of Agricultural Economics, Agricultural College and Research Institute, TNAU, Madurai.
Published: July 2022

The study was executed in the Theni district of Tamil Nadu by selecting 4 major vegetable-producing blocks, 100 vegetable farmers have been drawn proportionately and the data were gathered using a wellstructured and pre-tested interview schedule and analyzed using SPSS software. The study aims at assessing and comparing the awareness and adoption levels of vegetable farmers regarding safe pesticide handling practices. Among the selected vegetable farmers, a majority (73%) of the vegetable farmers have a medium level of awareness about safe pesticide handling practices. At the same time, majority (51%) of the vegetable farmers have a low level of adoption. It can be interpreted from the study that most vegetable farmers were aware, but they were not properly adopting safe pesticide handling practices.
THE ISSUE OF QAWAMAH IN TRADITIONAL AND MODERN LEGAL DISCOURSE
Authors:   Visiting Professor of Law University of Management & Technology, Lahore, Hassan Abida , Fatima Samza ,   Assistant Professor of Law, Bahauddin Zakariya University, Multan, Bilal Muhammad ,   Assistant Professor of Law, Bahauddin Zakariya University, Multan
Published: July 2022

The present study discusses the concept of Qawamah (leadership in the family), in modern society and its legal discourse. The term ‘Qawamah’ which means males are protectors of females and they are also responsible to provide a peaceful environment in society. Family Law has been subject to a far greater degree of codification. This topic has been under discussion in different times and this research scans gender (husband and wife) roles and codification of family laws in Pakistan as a trail for protecting the rights of women as well as men. The conception of guardianship has provided the basis of gender (husband and wife) roles in Muslim societies. Women are often expected to be submissive and loyal wives and mothers, living within the family, and men are normally taken as to be defenders and custodians of the family. The fairness of this system lies in the fact that Allah favored the man with the necessary abilities and services for the 'guardianship' and also handed over him with the responsibility to provide for the arrangement's upkeep. This research validates the constitutional, Quar’anic, and global reference points. Following some diverse sources, and while legal codification in much of the Islamic world has introduced fundamentally male-controlled legal norms. This whole discussion provides a wider context of an organized and codified legal system in the Muslim world.
HINDUTVA AND THE RISE OF THE CONTEMPORARY HINDU NATIONALISM (WITH SPECIAL REFERENCE TO ISLAMIC THOUGHTS AND MUSLIM OF INDIA)
Authors:   Assistant Professor, International Islamic University, Islamabad, Pakistan, Brohi Abdul Fareed
Published: July 2022

Muslims and Hindus are living with each other for hundreds of years. During this time many kinds of historical, political, cultural and religious conflicts occurred. The impact and the consequences of these conflicts are very much. In the late nineteenth and early twentieth centuries, Hindu traditions have been followed and understood in new ways. Many changes took place in Hinduism and it faced many challenges in different aspects .These changes were of political as well as well as cultural and traditional. Many reforms took place in these periods. In result, a particular crystallization of Hinduism-based Ideology occurred.
IMPORTANCE OF DA'WAH IN ISLAM: A REVIEW OF THE PROPHET'S DA'WAH STRATEGIES IN STATE OF MADINA
Authors:   Lecturer Islamic Studies, Department of Pakistan Studies, Abbottabad University of Science and Technology, Abbottabad, Jaffar Saad
Published: July 2022

Islam is a peaceful religion, teaches love, honesty and brotherhood to all humanity. Propagation of Islam by useful means calls Da'wah and it is one of the most favorite strategies of Holy Prophet (SAW), for the propagation of Islam, He used various methods and won the hearts and minds of people. His Madni life is a golden era of His life, in which He applied new and good strategies, and with a short span of time conquest of Makah made possible and light of Islam was spread all over the World. The current research paper shares valuable knowledge about the Prophetic Madni Da’wah strategies and also highlights the importance of dawah in day to day life with appropriate examples. The current research work is qualitative and descriptive in nature based upon both primary and secondary sources e.g. Quran Pak, Ahadith-e-Nabvi, Tafaseer, books and published research papers in various journals. The paper also aims to discuss the need and importance of Prophetic Da’wah strategies in our life. The study mainly focuses on the Da'wah strategies of Holy Prophet which need to be addressed and should be implementing in 21st century. Furthermore, the life of Holy Prophet is a great source of learning, so His Da'wah techniques must be learned and adopted because the preaching of Islam is a duty of all Muslim men and women. This paper provides immense knowledge about Prophetic Madni Da'wah techniques which are the fruitful examples for all mankind.
Pengembangan UMKM pada Masa Pandemi Covid-19 dan Pasca Banjir Melalui Budidaya Lele dan Jamur Tiram
Authors: Wiguna Frans Aditia , Permana Erwin Putera , Imron Ilmawati Fahmi , Putri Kharisma Eka , Damariswara Rian , Damayanti Susi , Hapsari Devinsa Pinda
Published: July 2022

Desa Cengkok, Kecamatan Tarokan, Kabupaten Kediri mengalami penurunan kualitas maupun kuantitas pendapatan masyarakat. Selain terdampak dari pandemi covid juga seringnya terjadi bencana banjir. Selain dampak material maupun non material juga berdampak langsung pada sektor ekonomi di desa tersebut. Untuk meningkatkan sektor ekonomi, Tim pengabdian tergerak untuk ikut membantu mencari jalan keluar dari permasalahan Desa Cengkok khususnya sektor ekonomi. Tujuan dari pengabdian ini adalah dengan melaksanakan pendampingan dan pengembangan UMKM agar masyarakat dapat meningkatkan perekonomian dan masyarakat tetap bisa menjalankan usaha ditengah pandemi covid-19. Metode yang digunakan yaitu tahap perencanaan, tahap persiapan, tahap pelaksanaan kegiatan dan tahap evaluasi. Hasil yang dicapai yaitu sosialisasi kewirausahaan UMKM, sosialisasi pemberdayaan lele, pembagian bibit lele kepada sebagian masyarakat. Dengan adanya Sosialisasi Kewirausahaan UMKM, Sosialisasi Pemberdayaan Ikan Lele, dan pembagian bibit lele dapat meningkatkan perekonomian masyarakat Desa Cengkok di masa pandemi covid-19 maupun pasca banjir.
Implementasi Sosial Media Marketing dalam Mendukung Kegiatan Pemasaran Pavin Block Berbahan Baku Sampah Plastik Kelompok “Lestari Bumi” Purbalingga
Authors: Santoso Muhammad Hery , Martiani Evi , Mutiasari Mutiasari , Rahmawati Herni Utami , Hasanah Nurul
Published: July 2022

Kegiatan Pengabdian Masyarakat ini menjalin kerjasama dengan mitra yaitu Kelompok Lestari Bumi suatu usaha produksi pavin block berbahan baku sampah plastik, dirintis oleh sekelompok warga desa Jetis kecamatan Kemangkon kabupaten Purbalingga yang mempunyai kesadaran akan semakin bertambahnya sampah plastik di daerahnya yang mengganggu lingkungan. Untuk itu mereka berupaya mengolahnya menjadi suatu produk yang bermanfaat dan mempunyai nilai ekonomis berupa pavin block. Kendala yang dihadapi kelompok Lestari Bumi adalah pada bidang pemasaran yang masih menggunakan metode tradisional yaitu dari mulut ke mulut mengakibatkan produk kurang dikenal masyarakat. Padahal dengan keunikan, kualitas produk dan harga ekonomis yang ditawarkannya dapat mendatangkan keuntungan apalagi bahan bakunya dari sampah plastik. Pelaksanaan Pengabdian Masyarakat menggunakan beberapa metode yaitu (1) Identifikasi kendala pemasaran mitra, (2) merumuskan solusi masalah mitra (3) melakukan pelatihan dan pendampingan terhadap mitra. Solusi yang dirumuskan adalah menerapkan pemasaran digital melalui jejaring sosial media Facebook. Hasil yang diharapkan (1) promosi produk dengan jangkauan yang lebih luas secara online melalui jejaring sosial media Facebook, (2) efisiensi biaya pemasaran, (3) meningkatnya kinerja penjualan dalam jangka panjang.
Pelatihan dan Pendampingan Model SCL (Student Center Learning) Berbasis Lesson Study Content Learning Loss di SDN 005 Tarakan
Authors: Kartini Kartini , Wahid Sucahyo Mas’an Al , Andari Kadek Dewi Wahyuni , Pratiwi Enditiyas , Noer Roby Zulkarnain , Agustina Degi Alrinda , Ramdhan Donna
Published: July 2022

Model pembelajaran berpusat pada peserta didik (SCL) telah dirilis oleh pemerintah dengan variasi contoh sintaksis di setiap jenjang pendidikan dan cukup memberikan ruang bagi modifikasi, inovasi dan kreativitas namun belum banyak dilakukan oleh sekolah. Terkait kurangnya pelatihan dalam penerapan model pembelajaran student center learning yang menyebabkan minimnya perencanaan yang dituangkan pada rencana pembelajaran di Sekolah sehingga belum terlihat refleksi yang jelas dan cocok untuk dilaksanakan pada sekolah dasar di kota Tarakan. Tujuan pengabdian yaitu sebagai tindaklanjut sosialisasi dan penerapan model-model pembelajaran yang berpusat kepada siswa pada abad 21. Metode pelaksanaan kegiatan dilaksanakan secara daring berupa pemodelan flipped classroom serta untuk penerapan Lesson Study dilaksanakan kegiatan secara luring berdasarkan hasil brainstroaming analisis video. Sasaran dan target dalam permasalahan tersebut adalah sebagai berikut: 1) Brainstroaming penerapan model SCL; 2) Pendampingan pembuatan plan, do, see berbasis daring; 3) Monitoring dan evaluasi Learning Loss; 4) Pembentukan Grup WhatsApp konten Model SCL di sekolah. Hasil yang ditemukan pada saat pengabdian, yakni: 1) Pelatihan penerapan model pembelajaran perlu untuk dipelajari mengingat semakin berkembangan ilmu pengetahuan dan cara mendapatkan informasi; 2) Kesiapan peserta dalam mengikuti workshop cukup baik; 3) Beberapa peserta aktif konsultasi melalui Grup WhatsApp; 4) Peserta mampu mengerjakan tugas sesuai dengan waktu yang ditentukan mulai dari identifikasi model SCL sampai menerapkannya yang sesuai dengan media pembelajaran offline dan online. Simpulan pada hasil pengabdian yaitu pendidik mampu menentukan taksonomi bloom sesuai dengan materi dan karakteristik peserta didik melalui model pembelajaran berpusat pada peserta didik.
Pengenalan Penerapan Ergonomi Kantor dan 5S Kepada Pegawai Administrasi Kelurahan X
Authors: Sarvia Elty , Halim Winda , Novi Novi , Yudiantyo Wawan , Christina Christina , Soly Silviana
Published: July 2022

Ergonomi kantor berbicara bagaimana membuat area kerja di kantor dapat sesuai untuk orang yang bekerja dan mendukung aktivitas pekerjaan yang dilakukannya. Sedangkan 5S (Seiri, Seiton, Seiso, Seiketsu, dan Shitsuke) adalah sebuah sistem yang membuat aktivitas kerja menjadi lebih baik dan berkualitas. Jika kedua prinsip ini diterapkan dengan baik pada sebuah aktivitas kantor tentu akan meningkatkan efisiensi dan efektifitas pekerjaan yang dilakukan. Pengenalan kedua prinsip tersebut dilakukan untuk pegawai Kelurahan X. Informasi diberikan melalui format webinar interaktif melalui platform Zoom Meeting. Tujuan dari kegiatan ini adalah memberikan pemahaman mengenai pentingnya Ergonomi di kantor dan pentingnya implementasi 5S kepada karyawan kantor kelurahan X. Pada tahap awal pelaksanaan informasi diberikan melalui metode ceramah dan melakukan pengumpulan data terkait pengetahuan yang peserta miliki saat ini dengan kuesioner menggunakan Google Form, kemudian pada tahap berikutnya peserta diminta untuk ikut berpartisipasi secara aktif terkait penerapan materi yang telah disampaikan sebelumnya. Pada hasil akhir pengumpulan data terbukti bahwa peserta telah memahami manfaat penerapan prinsip tersebut dengan meningkatkan prosentase pemahaman peserta yang diperoleh dari kuesioner akhir.