bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploring the opportunities and risks of aerial monitoring for biodiversity conservation
Authors: Millner Naomi , Cunliffe Andrew M. , Mulero-Pázmány Margarita , Newport Ben , Sandbrook Chris , Wich Serge
Published: June 2023

Drones are unoccupied aerial systems (UAS) whose technology has evolved rapidly over the past 15 years. Increasingly used in conservation to manage and monitor biodiversity, drones offer rich capabilities to observe in difficult terrain, have relatively affordable hardware costs and are likely to continue to proliferate rapidly in the years ahead. Drones are useful for tasks as diverse as monitoring wildlife poaching and illegal timber extraction, managing ecotourism and disaster responses, and tracking the regeneration or degradation of forests, and offer potential for more specialised tasks as their sensory payloads are developed. However, although associated technical issues and applications have been explored in wide-ranging ways within conservation science, there has been relatively little social-scientific engagement with drones to date. This leaves a gap surrounding the potential social benefits and risks of drones, as well as in interdisciplinary conversations. This introduction is the first of four papers under the heading ‘Drone ecologies’, building on an interdisciplinary workshop held under the same name at the University of Bristol in July 2021. Expanding from the plenary dialogues that opened this workshop, this introduction explores what interdisciplinary perspectives on drones can offer in addressing global social and ecological challenges, drawing on expertise from the fields of conservation biology, human and physical geography, rainforest ecology and environmental systems. Setting out the aims of the overall special collection, we review here the ways that drones are being used, and might be used, in biodiversity conservation, setting out important considerations to minimise risks of inadvertent harms.
Miniaturised dual-modality all-optical ultrasound probe for laser interstitial thermal therapy (LITT) monitoring
Authors: Zhang Shaoyan , Bodian Semyon , Zhang Edward Z. , Beard Paul C. , Noimark Sacha , Desjardins Adrien E. , Colchester Richard J.
Published: June 2023

All-optical ultrasound (OpUS) has emerged as an imaging paradigm well-suited to minimally invasive imaging due to its ability to provide high resolution imaging from miniaturised fibre optic devices. Here, we report a fibre optic device capable of concurrent laser interstitial thermal therapy (LITT) and real-time in situ all-optical ultrasound imaging for lesion monitoring. The device comprised three optical fibres: one each for ultrasound transmission, reception and thermal therapy light delivery. This device had a total lateral dimension of <1 mm and was integrated into a medical needle. Simultaneous LITT and monitoring were performed on ex vivo lamb kidney with lesion depth tracked using M-mode OpUS imaging. Using one set of laser energy parameters for LITT (5 W, 60 s), the lesion depth varied from 3.3 mm to 8.3 mm. In all cases, the full lesion depth could be visualised and measured with the OpUS images and there was a good statistical agreement with stereomicroscope images acquired after ablation (t=1.36, p=0.18). This work demonstrates the feasibility and potential of OpUS to guide LITT in tumour resection.
Shot noise reduction in radiographic and tomographic multi-channel imaging with self-supervised deep learning
Authors: Zharov Yaroslav , Ametova Evelina , Spiecker Rebecca , Baumbach Tilo , Burca Genoveva , Heuveline Vincent
Published: June 2023

Shot noise is a critical issue in radiographic and tomographic imaging, especially when additional constraints lead to a significant reduction of the signal-to-noise ratio. This paper presents a method for improving the quality of noisy multi-channel imaging datasets, such as data from time or energy-resolved imaging, by exploiting structural similarities between channels. To achieve that, we broaden the application domain of the Noise2Noise self-supervised denoising approach. The method draws pairs of samples from a data distribution with identical signals but uncorrelated noise. It is applicable to multi-channel datasets if adjacent channels provide images with similar enough information but independent noise. We demonstrate the applicability and performance of the method via three case studies, namely spectroscopic X-ray tomography, energy-dispersive neutron tomography, and in vivo X-ray cine-radiography.
Acoustic and plasma sensing of laser ablation via deep learning
Authors: Grant-Jacob James A. , Mills Ben , Zervas Michalis N.
Published: June 2023

Monitoring laser ablation when using high power lasers can be challenging due to plasma obscuring the view of the machined sample. Whilst the appearance of the generated plasma is correlated with the laser ablation conditions, extracting useful information is extremely difficult due to the highly nonlinear processes involved. Here, we show that deep learning can enable the identification of laser pulse energy and a prediction for the appearance of the ablated sample, directly from camera images of the plasma generated during single-pulse femtosecond ablation of silica. We show that this information can also be identified directly from the acoustic signal recorded during this process. This approach has the potential to enhance real-time feedback and monitoring of laser materials processing in situations where the sample is obscured from direct viewing, and hence could be an invaluable diagnostic for laser-based manufacturing.
Massively parallel FMCW lidar with cm range resolution using an electro-optic frequency comb
Authors: He Bibo , Zhang Chenbo , Yang Jiachuan , Chen Nuo , He Xuanjian , Tao Jinming , Zhang Zhike , Chu Tao , Chen Zhangyuan , Xie Xiaopeng
Published: June 2023

Frequency-modulated continuous wave (FMCW) light detection and ranging (lidar) is a promising solution for three-dimensional (3D) imaging and autonomous driving. This technique maps range and velocity measurement to frequency counting via coherent detection. Compared with single-channel FMCW lidar, multi-channel FMCW lidar can greatly improve the measurement rate. A chip-scale soliton micro-comb is currently used in FMCW lidar to enable multi-channel parallel ranging and significantly increase the measurement rate. However, its range resolution is limited due to the soliton comb having only a few-GHz frequency sweep bandwidth. To overcome this limitation, we propose using a cascaded modulator electro-optic (EO) frequency comb for massively parallel FMCW lidar. We demonstrate a 31-channel FMCW lidar with a bulk EO frequency comb and a 19-channel FMCW lidar using an integrated thin-film lithium niobate (TFLN) EO frequency comb. Both systems have a sweep bandwidth of up to 15 GHz for each channel, corresponding to a 1-cm range resolution. We also analyze the limiting factors of the sweep bandwidth in 3D imaging and perform 3D imaging for a specific target. The measurement rate achieved is over 12 megapixels per second, which verifies its feasibility for massively parallel ranging. Our approach has the potential to greatly benefit 3D imaging in fields where high range resolution is required, such as in criminal investigation and precision machining.
Optical coherent combining of high-power optical amplifiers for free-space optical communications
Authors: Billault Vincent , Leveque Simon , Maho Anaëlle , Welch Matthew , Bourderionnet Jérôme , Lallier Eric , Sotom Michel , Kernec Arnaud Le , Brignon Arnaud
Published: June 2023

Highly efficient coherent beam combining (CBC) of two very-high-power optical amplifiers (VHPOA) with applications to long-range FSO communications such as ground-to-space feeder links is presented. The CBC setup is designed to minimize the telecom signal degradation, with a polarization beam splitter used to minimize the power fluctuations and to control the output polarization state of the beam. The system delivers 80 W output power and is proven to be compatible with 25 Gb/s telecom signals with a less than 1 dB power penalty.
Extending orbital angular momentum multiplexing to radially high orders for massive mode channels in fiber transmission
Authors: Kong Aru , Lei Ting , Wang Dawei , Tu Jiajing , Shen Lei , Zhang Lei , Luo Jie , Fang Juncheng , Zhang Wanlong , Yuan Xiaocong
Published: June 2023

Orbital angular momentum (OAM) beams with different angular indices l have the potential to greatly increase communication capacity. However, the finite aperture of optical systems limits the value of the angular index. In order to fully use the orthogonal mode channels supported in the fiber for high-capacity communications, we propose extending the radial indices p of OAM modes as an additional multiplexing dimension. In this paper, we introduce spatially discrete multiple phase planes to multiplex the angular and radial OAM modes simultaneously. Due to the orthogonal property of the central symmetric OAM modes, a two-dimensional (2D) input Gaussian beams array can be converted to coaxial OAM modes through Cartesian to log-polar coordinate transformation by inverse design. For a proof-of-concept demonstration, a 10-mode multiplexer for high-order radial OAM modes was designed using five phase planes. The fabricated multiplexer generated high-quality multiplexed OAM modes with a loss of less than 5.4 dB. The multiplexed OAM modes were coupled into a specially designed ring-core fiber by mode-field matching, achieving stable mode transmission in 2 km fiber. The approach provides a scalable technology to increase the number of transmission channels and could lead to the practical applications of OAM multiplexing in communication.
Tunable non-reciprocal waveguide using spoof plasmon polariton coupling to a gaseous magnetoplasmon
Authors: Cappelli Mark A. , Mehrpour Bernety Hossein , Sun Daniel , Houriez Luc , Wang Benjamin
Published: June 2023

We experimentally demonstrate non-reciprocal (one-way) waveguiding in a microstrip transmission line tailored to support the propagation of spoof plasmon polaritons. Time-reversal symmetry is broken by coupling the microstrip fields to a magnetized gaseous plasma discharge column thereby exciting non-reciprocal magnetoplasmons at the interface between the plasma and a surrounding quartz envelope. The magnetic bias introduces asymmetry in the dispersion of the surface plasmon polaritons at the gaseous plasma–dielectric interface, resulting in a breaking of the bidirectionality of the wave propagation in the microstrip. The isolation generated at conditions of modest magnetic bias is measured to be nearly 60 dB, and tunable by varying the plasma density through the voltage applied to the discharge. The advantage of using magnetized gaseous plasmas to produce this unidirectional waveguide structure is that it can be turned on or off at rates limited by the production and recombination of the plasma.
Investigation of Ga2O3-BaO-GeO2 glasses for ultrafast laser inscription
Authors: Guérineau Théo , Dupont Albert , Lapointe Jerome , Vallée Réal , Messaddeq Younès
Published: June 2023

From the perspective of developing robust mid-infrared (mid-IR) integrated photonic devices, barium-gallium-germanium (BGG) oxide glasses are strong candidates among other mid-IR glasses. Indeed, compared to fluoride, tellurite or chalcogenide glasses, BGG glasses present the highest thermal and chemical stabilities, while transmitting light up to 6 µm. In parallel to this, technological advances in ultrafast direct laser writing (UDLW)-based devices are driving the development of novel photonic glasses. Specifically, there is a need to identify the most efficient mid-infrared transmitting BGG glass compositions for sustaining the UDLW process. In this article, we thoroughly investigate the BGG physicochemical properties through absorption and Raman spectroscopies, refractive index, density, and glass transition temperature measurements in two relevant glass series: one via a Ga3+/Ge4+ ratio fixed to 1 and a barium content varying from 25 to 40 cationic percent, the other via a 2Ba2+/Ga3+ ratio fixed to 1 and a germanium content varying from 20 to 80 cationic percent. In the meantime, we explore the photosensitivity of these glasses under UDLW. Our findings reveal the valuable role of both barium and gallium ions, notably through their concentration, structural stabilization sites and viscosity influence. Finally, we demonstrate the fabrication of an 8.2 cm-long UDLW-induced waveguide with propagation losses of < 0.3 dB.cm-1 at 1550 nm.
Narrow bandwidth perfect absorber based on composite hybrid plasmonics
Authors: Li Tengfei , Helmy Amr S. , Liu Haiyun , Xiong Qihua
Published: June 2023

Surface plasmon polaritons (SPPs) have shown unprecedented potential for miniaturizing photonic devices beyond the optical diffraction limit. However, their high intrinsic loss from metal material has been a major barrier to practical applications. One effective approach to reducing their effective loss is to engineer SPPs coupling by designing hybrid plasmonic waveguides (HPWs). In this work, we first theoretically calculate and analyze the coupling modes in a composite HPW (CHPW), demonstrating that the long-range and short-range supermodes in CHPW correspond to two different SPPs at two metal layer interfaces. Next, we propose and demonstrate a narrow bandwidth perfect absorber based on CHPW structure. The absorber’s bandwidth is only 12.9 nm in the visible range and 6.67 nm in the near-infrared range. Our simulation results demonstrate that the CHPW’s SPPs coupling mechanism can be extended to localized surface plasmons (LSPs) in the proposed perfect absorber, enabling a low-loss mode that contributes to the narrow bandwidth. This suggests that the CHPW configuration could facilitate the development of more advanced low-loss, high-performance plasmonic devices.
Stochastic simulations of self-organized elastogenesis in the developing lung
Authors: Fan Xiru , Valenzuela Cristian , Zhao Weijing , Chen Zi , Wang Dong , Mentzer Steven J.
Published: June 2023

In the normal lung, the dominant cable is an elastic “line element” composed of elastin fibers bound to a protein scaffold. The cable line element maintains alveolar geometry by balancing surface forces within the alveolus and changes in lung volume with exercise. Recent work in the postnatal rat lung has suggested that the process of cable development is self-organized in the extracellular matrix. Early in postnatal development, a blanket of tropoelastin (TE) spheres appear in the primitive lung. Within 7 to 10 days, the TE spheres are incorporated into a distributed protein scaffold creating the mature cable line element. To study the process of extracellular assembly, we used cellular automata (CA) simulations. CA simulations demonstrated that the intermediate step of tropoelastin self-aggregation into TE spheres enhanced the efficiency of cable formation more than 5-fold. Similarly, the rate of tropoelastin production had a direct impact on the efficiency of scaffold binding. The binding affinity of the tropoelastin to the protein scaffold, potentially reflecting heritable traits, also had a significant impact on cable development. In contrast, the spatial distribution of TE monomer production, increased Brownian motion and variations in scaffold geometry did not significantly impact simulations of cable development. We conclude that CA simulations are useful in exploring the impact of concentration, geometry, and movement on the fundamental process of elastogenesis.
Development of AlissAID system targeting GFP or mCherry fusion protein
Authors: Ogawa Yoshitaka , Nishimura Kohei , Obara Keisuke , Kamura Takumi
Published: June 2023

Conditional control of target proteins using the auxin-inducible degron (AID) system provides a powerful tool for investigating protein function in eukaryotes. Here, we established an Affinity-linker based super-sensitive auxin-inducible degron (AlissAID) system in budding yeast by using a single domain antibody (a nanobody). In this system, target proteins fused with GFP or mCherry were degraded depending on a synthetic auxin, 5-Adamantyl-IAA (5-Ad-IAA). In AlissAID system, nanomolar concentration of 5-Ad-IAA induces target degradation, thus minimizing the side effects from chemical compounds. In addition, in AlissAID system, we observed few basal degradations which was observed in other AID systems including ssAID system. Furthermore, AlissAID based conditional knockdown cell lines are easily generated by using budding yeast GFP Clone Collection. Target protein, which has antigen recognition sites exposed in cytosol or nucleus, can be degraded by the AlissAID system. From these advantages, the AlissAID system would be an ideal protein-knockdown system in budding yeast cells.
IFT74 variants cause skeletal ciliopathy and motile cilia defects in mice and humans
Authors: Bakey Zeineb , Cabrera Oscar A. , Hoefele Julia , Antony Dinu , Wu Kaman , Stuck Michael W. , Micha Dimitra , Eguether Thibaut , Smith Abigail O. , van der Wel Nicole N. , Wagner Matias , Strittmatter Lara , Beales Philip L. , Jonassen Julie A. , Thiffault Isabelle , Cadieux-Dion Maxime , Boyes Laura , Sharif Saba , Tüysüz Beyhan , Dunstheimer Desiree , Niessen Hans W. M. , Devine William , Lo Cecilia W. , Mitchison Hannah M. , Schmidts Miriam , Pazour Gregory J.
Published: June 2023

Motile and non-motile cilia play critical roles in mammalian development and health. These organelles are composed of a 1000 or more unique proteins, but their assembly depends entirely on proteins synthesized in the cell body and transported into the cilium by intraflagellar transport (IFT). In mammals, malfunction of non-motile cilia due to IFT dysfunction results in complex developmental phenotypes that affect most organs. In contrast, disruption of motile cilia function causes subfertility, disruption of the left-right body axis, and recurrent airway infections with progressive lung damage. In this work, we characterize allele specific phenotypes resulting from IFT74 dysfunction in human and mice. We identified two families carrying a deletion encompassing IFT74 exon 2, the first coding exon, resulting in a protein lacking the first 40 amino acids and two individuals carrying biallelic splice site mutations. Homozygous exon 2 deletion cases presented a ciliary chondrodysplasia with narrow thorax and progressive growth retardation along with a mucociliary clearance disorder phenotype with severely shorted cilia. Splice site variants resulted in a lethal skeletal chondrodysplasia phenotype. In mice, removal of the first 40 amino acids likewise results in a motile cilia phenotype but with little effect on primary cilia structure. Mice carrying this allele are born alive but are growth restricted and developed hydrocephaly in the first month of life. In contrast, a strong, likely null, allele ofIft74in mouse completely blocks ciliary assembly and causes severe heart defects and midgestational lethality.In vitrostudies suggest that the first 40 amino acids of IFT74 are dispensable for binding of other IFT subunits but are important for tubulin binding. Higher demands on tubulin transport in motile cilia compared to primary cilia resulting from increased mechanical stress and repair needs could account for the motile cilia phenotype observed in human and mice.
Effect of COVID-19 pandemic on the incidence of acute diarrheal disease and pneumonia among under 5 children in Ethiopia- A database study
Authors: Negsso Abebe , Arega Balew , Abdissa Fekadu , Zewdu Brook , Teshome Ayele , Minda Abrham , Agunie Asnake
Published: June 2023

COVID-19 has had a devastating impact on preventable and treatable pediatric diseases in Ethiopia. This study looks at the impact of COVID-19 on pneumonia and acute diarrheal diseases in the country, as well as the differences between administrative regions. In Ethiopia, we conducted a retrospective pre-post study to assess the impact of COVID-19 on children under the age of five who had acute diarrhea and pneumonia and were treated in health facilities during the pre-COVID-19 era (March 2019 to February 2020) and the COVID-19 era (March 2020 to February 2021). From the National Health Management District Health Information System (DHIS2, HMIS), we retrieved data on total acute diarrheal disease and pneumonia, along with their regional and monthly distribution. We calculated incidence rate ratios comparing the rates of acute diarrhea and pneumonia during the pre-and post-COVID-19 eras and adjusted for the year, using Poisson regression. The number of under-five children treated for acute pneumonia decreased from 2,448,882 before COVID-19 to 2,089,542 ((14.7% reduction (95%CI;8.72–21.28), p<0.001)) during COVID-19. Similarly, the number of under-five children treated for acute diarrheal disease decreased from 3,287,850 in pre-COVID-19 to, 2,961,771((9.91% reduction (95%CI;6.3–17.6%),p<0.001)) during COVID-19. In the majority of the administrative regions studied, pneumonia and acute diarrhea diseases decreased during COVID-19, but they increased in Gambella, Somalia, and Afar. During the COVID-19 period, the greatest reduction of children with pneumonia (54%) and diarrhea disease (37.3%) was found in Addis Ababa (p<0.001). The majority of administrative regions included in this study have seen a decrease in pneumonia and acute diarrheal diseases among children under the age of five, while three regions namely, Somalia, Gambela, and Afar saw an increase in cases during the pandemic. This emphasizes the importance of using tailored approaches in mitigating the impact of infectious diseases such as diarrhea and pneumonia during situations of a pandemic such as COVID-19.
Utilization of health insurance by patients with diabetes or hypertension in urban hospitals in Mbarara, Uganda
Authors: Kangwagye Peter , Bright Laban Waswa , Atukunda Gershom , Basaza Robert , Bajunirwe Francis
Published: June 2023

Background

Diabetes and hypertension are among the leading contributors to global mortality and require life-long medical care. However, many patients cannot access quality healthcare due to high out-of-pocket expenditures, thus health insurance would help provide relief. This paper examines factors associated with utilization of health insurance by patients with diabetes or hypertension at two urban hospitals in Mbarara, southwestern Uganda.

Methods

We used a cross-sectional survey design to collect data from patients with diabetes or hypertension attending two hospitals located in Mbarara. Logistic regression models were used to examine associations between demographic factors, socio-economic factors and awareness of scheme existence and health insurance utilization.

Results

We enrolled 370 participants, 235 (63.5%) females and 135 (36.5%) males, with diabetes or hypertension. Patients who were not members of a microfinance scheme were 76% less likely to enrol in a health insurance scheme (OR = 0.34, 95% CI: 0.15–0.78, p = 0.011). Patients diagnosed with diabetes/hypertension 5–9 years ago were more likely to enrol in a health insurance scheme (OR = 2.99, 95% CI: 1.14–7.87, p = 0.026) compared to those diagnosed 0–4 years ago. Patients who were not aware of the existing schemes in their areas were 99% less likely to take up health insurance (OR = 0.01, 95% CI: 0.0–0.02, p < 0.001) compared to those who knew about health insurance schemes operating in the study area. Majority of respondents expressed willingness to join the proposed national health insurance scheme although concerns were raised about high premiums and misuse of funds which may negatively impact decisions to enrol.

Conclusion

Belonging to a microfinance scheme positively influences enrolment by patients with diabetes or hypertension in a health insurance program. Although a small proportion is currently enrolled in health insurance, the vast majority expressed willingness to enrol in the proposed national health insurance scheme. Microfinance schemes could be used as an entry point for health insurance programs for patients in these settings.
Ethical and methodological reflections: Digital storytelling of self-care with students during the COVID-19 pandemic at a South African University
Authors: Gumede Dumile , Sibiya Maureen Nokuthula
Published: June 2023

The enforcement of the coronavirus disease 2019 (COVID-19) pandemic restrictions disrupted the traditional face-to-face qualitative data collection in public health. The pandemic forced qualitative researchers to transition to remote methods of data collection such as digital storytelling. Currently, there is a limited understanding of ethical and methodological challenges in digital storytelling. We, therefore, reflect on the challenges and solutions for implementing a digital storytelling project on self-care at a South African university during the COVID-19 pandemic. Guided by Salmon’s Qualitative e-Research Framework, reflective journals were used in a digital storytelling project between March and June 2022. We documented the challenges of online recruitment, obtaining informed consent virtually, and collecting data using digital storytelling as well as the efforts of overcoming the challenges. Our reflections identified major challenges, namely online recruitment and informed consent compromised by asynchronous communication; participants’ limited research knowledge; participants’ privacy and confidentiality concerns; poor internet connectivity; quality of digital stories; devices with a shortage of storage space; participants’ limited technological skills; and time commitment required to create digital stories. Strategies adopted to address these challenges included an ongoing informed consent process; flexible timelines for the creation of digital stories; one-on-one guidance on creating digital stories; and multiple online platforms to share digital stories. Our critical reflection offers practical guidance for the ethical conduct of digital storytelling in public health research and makes a significant contribution to methodological considerations for use in future pandemics. These ethical and methodological challenges should be recognized as features of the context of the research setting including restrictions imposed by the COVID-19 pandemic than disadvantages of digital storytelling.
Timeliness and missed opportunities for vaccination among children aged 0 to 23 months in Dschang health district, West region, Cameroon: A cross-sectional survey
Authors: Dombou Zeufack Arsène Gautler , Noukeu Njinkui Diomede , Whegang Youdom Solange , Ateudjieu Jérôme
Published: June 2023

Missed opportunities for vaccination (MOV) reflect quality of immunization service. The objective of this study was to assess vaccination timeliness, prevalence, and characteristics of MOVs among children aged 0–23 months, as well as knowledge, attitude and practice of health workers towards immunization. An exit interview method was used to select caregivers and health personnel. Selection took place in 26 health facilities within 14 health areas in the Dshcang Health district. Data were collected using two face-to-face questionnaires adapted from the World Health Organization (WHO) tools. We conducted an evaluation of all free vaccines in the Expanded Programme on Immunisation (EPI). We studied timeliness, assessed MOV, and knowledge, behaviour and attitude of health workers on immunization. Basic statistical tests were used to study the association between MOV and socio demographic characteristics. A total of 363 children aged 0 to 23 months were surveyed. A total of 88 (91.66%) of health personnel agreed to participate in our study. A total of 298 (82.1%) children had vaccination cards with dates, leading to 18% not completely vaccinated. Vaccination timeliness ranged from 20% to 77%. Overall MOV estimated was 23.83%, range from 0% to 16.4% among all vaccines. Among health workers, 70.45% (62/88) had insufficient knowledge on vaccination, 73.86% assessed the vaccination status of children during any routine visit and 74% ask parents to bring the child’s vaccination record to any health facility visit. The study highlighted presence of MOV among children. Strategies for remedying this includes strengthening parents’ knowledge, organizing refresher courses for health workers on vaccination, and systematically assessing children’s vaccination status.
Chagas prevention and control in an endemic area from the Argentinian Gran Chaco Region: Data from 14 years of uninterrupted intervention
Authors: Weinberg Diego , Casale Maria Florencia , Cejas Rosa Graciela , Hoyos Rafael , Periago María Victoria , Segura Elsa , Abril Marcelo Claudio
Published: June 2023

Background

Chagas Disease (ChD) is a Neglected Tropical Disease (NTD) affecting 6 to 7 million people worldwide, mostly from Latin America. In Argentina, a national control program has been implemented since 1962, yet there are still an estimated 1.6 million infected individuals. Control programs were based almost exclusively on entomological surveillance and chemical control of households and were not continuous given a lack of coordination and resources. Argentina´s ChD program was originally vertical and centralized; later, it was partially and, in general, unsuccessfully transferred to the provinces. Herein, we describe the implementation of a control program for ChD with an ecohealth approach in rural settlements around the city of Añatuya, Santiago del Estero.

Methods

The program included yearly household visits for entomological surveillance and control, health promotion workshops, and structural house improvements. Improved structures included internal and external walls and roofs, as well as the construction of water wells and latrines, and the organization and improvement of peri-domestic structures. Activities were carried out by specifically trained personnel except for house improvements, which were performed by the community, under technical guidance and provision of materials. Data was collected using standardized questionnaires for household characterization, entomological infestation status and chemical control activities.

Results

This program was continuously implemented since 2005 with high community participation and adherence, incorporating 13 settlements and 502 households. During the surveillance phase, 4,193 domiciliary inspections were performed, and both the intra- and peri-domestic infestation rate were reduced from 17.9% to 0.2% (P < 0.01) and from 20.4% to 3%, respectively. Additionally, 399 households were structurally improved.

Conclusion

The program is still ongoing and, after 14 years of implementation, has built social networks and collaboration between implementers and beneficiaries with a reduction of T. infestans infestation in the intra- and peri-domicile. This reduction, especially inside the household, has enabled access to diagnosis and treatment of the population, with minimal risk of re-infection.
Primary postpartum haemorrhage and longer-term physical, psychological, and psychosocial health outcomes for women and their partners in high income countries: A mixed-methods systematic review
Authors: Latt Su Mon , Alderdice Fiona , Elkington Madeline , Awng Shar Mahkawnghta , Kurinczuk Jennifer J. , Rowe Rachel
Published: June 2023

Objectives

Most research about outcomes following postpartum haemorrhage (PPH) has focused on immediate outcomes. There are fewer studies investigating longer-term maternal morbidity following PPH, resulting in a significant knowledge gap. This review aimed to synthesize the evidence about the longer-term physical and psychological consequences of primary PPH for women and their partners from high income settings.

Methods

The review was registered with PROSPERO and five electronic databases were searched. Studies were independently screened against the eligibility criteria by two reviewers and data were extracted from both quantitative and qualitative studies that reported non-immediate health outcomes of primary PPH.

Results

Data were included from 24 studies, of which 16 were quantitative, five were qualitative and three used mixed-methods. The included studies were of mixed methodological quality. Of the nine studies reporting outcomes beyond five years after birth, only two quantitative studies and one qualitative study had a follow-up period longer than ten years. Seven studies reported outcomes or experiences for partners. The evidence indicated that women with PPH were more likely to have persistent physical and psychological health problems after birth compared with women who did not have a PPH. These problems, including PTSD symptoms and cardiovascular disease, may be severe and extend for many years after birth and were more pronounced after a severe PPH, as indicated by a blood transfusion or hysterectomy. There was limited evidence about outcomes for partners after PPH, but conflicting evidence of association between PTSD and PPH among partners who witnessed PPH.

Conclusion

This review explored existing evidence about longer-term physical and psychological health outcomes among women who had a primary PPH in high income countries, and their partners. While the evidence about health outcomes beyond five years after PPH is limited, our findings indicate that women can experience long lasting negative impacts after primary PPH, including PTSD symptoms and cardiovascular disease, extending for many years after birth.

PROSPERO registration

PROSPERO registration number: CRD42020161144
A national professional development program fills mentoring gaps for postdoctoral researchers
Authors: Sun Ting , Drane Denise , McGee Richard , Campa Henry , Goldberg Bennett B. , Hokanson Sarah Chobot
Published: June 2023

The Postdoc Academy:Succeeding as a Postdocwas designed to build postdocs’ skills in career transition, career planning, collaborative research, resilience, and self-reflection. This study examined self-reported changes in five skills as learners progressed through the course. Data were collected from participants who responded to both pre- and post-surveys and engaged with the course learning activities. Results from repeated measures multivariate analysis of variance revealed that all of the self-reported perceptions of skills improved significantly upon completion of the course. Hierarchical regressions revealed that underrepresented minority learners had greater gains in their development of skills in career planning, resilience, and self-reflection. Qualitative analysis of learners’ responses to learning activities found that postdocs perceived networking and mentor support as contributing factors to their skill advancement while tensions among multiple obligations and concerns of uncertainties were significant challenges to applying those skills.
Automated segmentation and quantitative analysis of organelle morphology, localization and content using CellProfiler
Authors: Laan Sebastiaan N. J. , Dirven Richard J. , Bürgisser Petra E. , Eikenboom Jeroen , Bierings Ruben ,   for the SYMPHONY consortium
Published: June 2023

One of the most used and versatile methods to study number, dimensions, content and localization of secretory organelles is confocal microscopy analysis. However, considerable heterogeneity exists in the number, size and shape of secretory organelles that can be present in the cell. One thus needs to analyze large numbers of organelles for valid quantification. Properly evaluating these parameters requires an automated, unbiased method to process and quantitatively analyze microscopy data. Here, we describe two pipelines, run by CellProfiler software, called OrganelleProfiler and OrganelleContentProfiler. These pipelines were used on confocal images of endothelial colony forming cells (ECFCs), which contain unique secretory organelles called Weibel-Palade bodies (WPBs), and on early endosomes in ECFCs and human embryonic kidney 293T (HEK293T) cells. Results show that the pipelines can quantify the cell count, size, organelle count, organelle size, shape, relation to cells and nuclei, and distance to these objects in both endothelial and HEK293T cells. Additionally, the pipelines were used to measure the reduction in WPB size after disruption of the Golgi and to quantify the perinuclear clustering of WPBs after triggering of cAMP-mediated signaling pathways in ECFCs. Furthermore, the pipeline is able to quantify secondary signals located in or on the organelle or in the cytoplasm, such as the small WPB GTPase Rab27A. Cell profiler measurements were checked for validity using Fiji. To conclude, these pipelines provide a powerful, high-processing quantitative tool for the characterization of multiple cell and organelle types. These pipelines are freely available and easily editable for use on different cell types or organelles.
Impact of statin withdrawal on perceived and objective muscle function
Authors: Peyrel Paul , Mauriège Pascale , Frenette Jérôme , Laflamme Nathalie , Greffard Karine , Dufresne Sébastien S. , Huth Claire , Bergeron Jean , Joanisse Denis R.
Published: June 2023

Background and aims

Statin-associated muscle symptoms (SAMS) are frequently reported. Nevertheless, few data on objective measures of muscle function are available. Recent data suggesting an important nocebo effect with statin use could confound such effects. The objective was to assess if subjective and objective measures of muscle function improve after drug withdrawal in SAMS reporters.

Methods

Patients (59 men, 33 women, 50.3±9.6 yrs.) in primary cardiovascular prevention composed three cohorts: statin users with (SAMS, n = 61) or without symptoms (No SAMS, n = 15), and controls (n = 16) (registered at clinicaltrials.gov, NCT01493648). Force (F), endurance (E) and power (P) of the leg extensors (ext) and flexors (fle) and handgrip strength (Fhg) were measured using isokinetic and handheld dynamometers, respectively. A 10-point visual analogue scale (VAS) was used to self-assess SAMS intensity. Measures were taken before and after two months of withdrawal.

Results

Following withdrawal, repeated-measures analyses show improvements for the entire cohort in Eext, Efle, Ffle, Pext and Pfle (range +7.2 to +13.3%, all p≤0.02). Post-hoc analyses show these changes to occur notably in SAMS (+8.8 to +16.6%), concurrent with a decrease in subjective perception of effects in SAMS (VAS, from 5.09 to 1.85). Fhg was also improved in SAMS (+4.0 to +6.2%) when compared to No SAMS (-1.7 to -4.2%) (all p = 0.02).

Conclusions

Whether suffering from “true” SAMS or nocebo, those who reported SAMS had modest but relevant improvements in muscle function concurrent with a decrease in subjective symptoms intensity after drug withdrawal. Greater attention by clinicians to muscle function in frail statin users appears warranted.

Trial registration

This study is registered in clinicaltrials.gov (NCT01493648).
A listener preference model for spatial sound reproduction, incorporating affective response
Authors: Moiragias George , Mourjopoulos John
Published: June 2023

This work presents results and models for listener preference to music delivered via different spatial reproduction formats, here via mono, stereo and multichannel (5.1-ch) reproduction. Although this problem has been researched in the past, the current work introduces an elaborate multistage experimental procedure which considers the contribution of listener-specific emotional responses (valence and arousal) to his/hers Overall Listening Experience (OLE). The test procedure registers the individual listener preference and familiarization with the content of each test audio sample. A spatial envelopment metric, extracted directly from each audio signal sample is utilized as attribute for the perceived differences between the 3 different systems. This attribute, along with listener content preference (for each music sample) and his/hers affective response attributes are combined into linear regression model(s) which can predict the dominant trends for rating OLE. A novel linear tree approach is also proposed which highlights additional associations between the attributes within this multidimensional space. Comparative performance analysis shows that the proposed linear tree approach also achieves improved predictions for OLE ratings.
Relationship between history of hormonal contraceptive use and anaemia status among women in sub-Saharan Africa: A large population-based study
Authors: Aboagye Richard Gyan , Okyere Joshua , Seidu Abdul-Aziz , Ahinkorah Bright Opoku , Budu Eugene , Yaya Sanni
Published: June 2023

Background

Anaemia among women has been reported to be a significant contributor to hemorrhage, exacerbated risk of stillbirths, miscarriages, and maternal mortalities. Hence, understanding the factors associated with anaemia is imperative to develop preventive strategies. We examined the association between history of hormonal contraceptive use and risk of anaemia among women in sub-Saharan Africa.

Methods

We analyzed data from the recent Demographic and Health Surveys (DHS) of sixteen countries in sub-Saharan Africa. Countries with recent DHSs conducted from 2015 to 2020 were included in the study. A total of 88,474 women of reproductive age were included. We used percentages to summarize the prevalence of hormonal contraceptives and anaemia among women of reproductive age. We used multilevel binary logistic regression analysis to examine the association between hormonal contraceptives and anaemia. We presented the results using crude odds ratio (cOR) and adjusted odds ratios (aOR), with their respective 95 percent confidence intervals (95% CIs).

Results

On the average, 16.2% of women are using hormonal contraceptives and this ranged from 7.2% in Burundi to 37.7% in Zimbabwe. The pooled prevalence of anaemia was 41%, ranging from 13.5% in Rwanda to 58.0% in Benin. Women who used hormonal contraceptives were less likely to be anaemic compared to those who were not using hormonal contraceptives (aOR = 0.56; 95%CI = 0.53, 0.59). At the country-level, hormonal contraceptive use was associated with a reduced likelihood of anaemia in 14 countries, except for Cameroon and Guinea.

Conclusion

The study underscores the importance of promoting the use of hormonal contraceptives in communities and regions that have a high burden of anaemia among women. Specifically, health promotion interventions aimed at promoting the use of hormonal contraceptives among women must be tailored to meet the needs of adolescents, multiparous women, those in the poorest wealth index, and women in union as these sub-populations were at significantly higher risk of anaemia in sub-Saharan Africa.
A mathematical model for cancer risk and accumulation of mutations caused by replication errors and external factors
Authors: Uchinomiya Kouki , Tomita Masanori
Published: June 2023

Replication errors influence mutations, and thus, lifetime cancer risk can be explained by the number of stem-cell divisions. Additionally, mutagens also affect cancer risk, for instance, high-dose radiation exposure increases lifetime cancer risk. However, the influence of low-dose radiation exposure is still unclear because this influence, if any, is very slight. We can assess the minimal influence of the mutagen by virtually comparing the states with and without mutagen using a mathematical model. Here, we constructed a mathematical model to assess the influence of replication errors and mutagens on cancer risk. In our model, replication errors occur with a certain probability during cell division. Mutagens cause mutations at a constant rate. Cell division is arrested when the number of cells reaches the capacity of the cell pool. When the number of cells decreases because of cell death or other reasons, cells resume division. It was assumed that the mutations of cancer driver genes occur stochastically with each mutation and that cancer occurs when the number of cancer driver gene mutations exceeds a certain threshold. We approximated the number of mutations caused by errors and mutagens. Then, we examined whether cancer registry data on cancer risk can be explained only through replication errors. Although the risk of leukemia was not fitted to the model, the risks of esophageal, liver, thyroid, pancreatic, colon, breast, and prostate cancers were explained only by replication errors. Even if the risk was explained by replication errors, the estimated parameters did not always agree with previously reported values. For example, the estimated number of cancer driver genes in lung cancer was larger than the previously reported values. This discrepancy can be partly resolved by assuming the influence of mutagen. First, the influence of mutagens was analyzed using various parameters. The model predicted that the influence of mutagens will appear earlier, when the turnover rate of the tissue is higher and fewer mutations of cancer driver genes were necessary for carcinogenesis. Next, the parameters of lung cancer were re-estimated assuming the influence of mutagens. The estimated parameters were closer to the previously reported values. than when considering only replication errors. Although it may be useful to explain cancer risk by replication errors, it would be biologically more plausible to consider mutagens in cancers in which the effects of mutagens are apparent.
Spatial ecology and conservation of leatherback turtles (Dermochelys coriacea) nesting in Bioko, Equatorial Guinea
Authors: Garzon Francesco , Barrientos Christian , Anvene Rigoberto Esono , Mba Feme Esono , Fallabrino Alejandro , Formia Angela , Godley Brendan J. , Gonder Mary K. , Prieto Carolina Martinez , Ayetebe Jesus Mba , Metcalfe Kristian , Montgomery David , Nsogo Juan , Nze Juan-Cruz Ondo , Possardt Earl , Salazar Erick Ross , Tiwari Manjula , Witt Matthew J.
Published: June 2023

Bioko Island (Equatorial Guinea) hosts important nesting habitat for leatherback sea turtles, with the main nesting beaches found on the island’s southern end. Nest monitoring and protection have been ongoing for more than two decades, although distribution and habitat range at sea remains to be determined. This study uses satellite telemetry to describe the movements of female leatherback turtles (n = 10) during and following the breeding season, tracking them to presumed offshore foraging habitats in the south Atlantic Ocean. Leatherback turtles spent 100% of their time during the breeding period within the Exclusive Economic Zone (EEZ) of Equatorial Guinea, with a core distribution focused on the south of Bioko Island extending up to 10 km from the coast. During this period, turtles spent less than 10% of time within the existing protected area. Extending the border of this area by 3 km offshore would lead to a greater than threefold increase in coverage of turtle distribution (29.8 ± 19.0% of time), while an expansion to 15 km offshore would provide spatial coverage for more than 50% of tracking time. Post-nesting movements traversed the territorial waters of Sao Tome and Principe (6.4%of tracking time), Brazil (0.85%), Ascension (1.8%), and Saint Helena (0.75%). The majority (70%) of tracking time was spent in areas beyond national jurisdiction (i.e. the High Seas). This study reveals that conservation benefits could be achieved by expanding existing protected areas stretching from the Bioko coastal zone, and suggests shared migratory routes and foraging space between the Bioko population and other leatherback turtle rookeries in this region.