bluuebunny/crossref_metadata_2025 huggingface dataset:
The influence of directional spreading on rogue waves triggered by abrupt depth transitions
Authors: Tang Tianning , Moss Charlotte , Draycott Samuel , Bingham Harry B. , van den Bremer Ton S. , Li Yan , Adcock Thomas A.A.
Published: October 2023

An increased number of rogue waves, relative to standard distributions, can be induced by unidirectional waves passing over abrupt decreases in water depth. We investigate this phenomenon in a more general setting of multidirectional waves. We examine the influence of the directionality on the occurrence probability of rogue waves using laboratory experiments and fully nonlinear potential flow simulations. Based on the analysis of the statistics of random waves, we find that directional spreading reduces the formation probability of rogue waves relative to unidirectional seas. Nevertheless, for typical values of directional spreading in the ocean (

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), our numerical results suggest that there is still a significant enhancement to the number of rogue waves just beyond the top of a depth discontinuity.
Women, blood, and dangerous things: socio-cultural variation in the conceptualization of menstruation
Authors: Vancauwenbergh Margot , Franco Karlien
Published: October 2023

This study examines a collection of expressions for the taboo topic of menstruation in Dutch, German, and Mandarin Chinese. A model for the identification of conceptualization patterns in taboo verbalizations is set up, analyzing each expression according to the X-phemistic mechanisms and, if applicable, the metaphorical source domains or metonymic vehicles at its origin. The various conceptualizations of menstruation are approached from a socio-cultural perspective; variation in conceptualization is examined through a correspondence regression analysis with three speaker-related explanatory variables (L1 and associated cultural background, menstrual experience, and age group). The underlying interest is linguo-cultural as the study aims to verify whether dominant menstrual attitudes are reflected in the linguistic conceptualization of menstruation within each socio-cultural group. Such correlations are indeed found, although the youngest age-group shows some unexpected linguistic behavior.
Exploring a Mallorca cave flooding during the Little Ice Age using nondestructive techniques on a stalagmite: micro-CT and XRF core scanning
Authors: Cisneros Mercè , Cacho Isabel , Frigola Jaime , Moreno Ana , Stoll Heather , Fornós Joan J. , Sigró Javier , Barriendos Mariano
Published: October 2023

This study focuses on characterizing a discontinuity within the Seán stalagmite (4.75–7.75 cm) by means of two nondestructive techniques: (1) high-resolution micro-computed tomography (micro-CT) and (2) X-ray fluorescence (XRF) core scanning (XRFCS). Micro-CT was used to study the stalagmite density, and XRFCS was applied to obtain the qualitative elemental composition and colour measurements. The new data obtained from nondestructive techniques have been combined with previously published geochemical data and fabric determinations from the same stalagmite found in Sa Balma des Quartó cave in Mallorca. The two methodologies applied in the present study have improved the characterization of the distinctive horizon. The micro-CT images identified the layer as a minor event due the high air content. The distinctive horizon is characterized by a high Ti-content, indicating the arrival of terrigenous particles. Based on those observations, together with the fact that the micrite layer appears filling the gaps between the older columnar fabric, we argue that the micrite layer may represent a major flooding event inside the cave after the year 1616 ± 23 CE and before the year 1623 ± 28 CE, which can be related to an extreme rainfall event. This hypothesis is further supported by the observed cave flooding during the autumn of 2018.
Information Spillover and Corporate Policies: The Case of Listed Options
Authors: Bernile Gennaro , Hu Jianfeng , Li Guangzhong , Michaely Roni
Published: October 2023

Information production associated with derivatives markets is not a sideshow; rather, it has significantly positive spillover effects on an array of corporate decisions of underlying firms. Using a regression-discontinuity design based on exogenous variation in options availability as an instrument for changes in the information environment, we show that options introductions have causal effects on corporate policies on both sides of the balance sheet. Through improved information efficiency, options availability reduces the need for debt and payout, increases efficient investment, and yields superior innovation. We conduct two independent experiments demonstrating that our instrument’s impact is not derived from alternative channels.
Strengthening primary health care in China: governance and policy challenges
Authors: Qian Jiwei , Ramesh M.
Published: October 2023

Primary care is often the weakest link in health systems despite its acknowledged central importance in promoting population's health at economical cost. A key reason for the lacunae is that both scholars and practitioners working on the subject typically underestimate the enormity of the task and the range of complementary measures required to build an effective primary care system. The objective of the paper is to highlight theoretical gaps and practical limitations to strengthening primary care. The challenges and difficulties are illustrated through a case study of China where primary care continues to struggle despite the government's strong political, financial and policy support in recent years. In this paper, we review the development of primary health care in China and how it is governed, provided, and financed, highlighting the gaps and misalignments that undermine its performance. We argue that governance deficiencies coupled with flawed financing and payments arrangements are major impediments to improving performance. China's experience offers valuable lessons for other governments seeking to strengthen primary health care.
Inkblots and the Life-World – Toward a Rorschach Phenomenology
Authors: McElfresh Patrick J.
Published: October 2023

Abstract: This article summarizes the historical influences on Hermann Rorschach’s Psychodiagnostics in light of new findings and the retranslation of his monograph by Keddy and colleagues ( Rorschach, 2021 ). This exposition situates his work in the historical context of Swiss culture and psychiatry at the time of his training and research, identifies theoretical influences on his thought given findings from recent scholarship, and describes collegial relationships that impacted his experiment. The potential influence of philosophical phenomenology on Hermann Rorschach’s work, had he not passed away prematurely, is postulated given his relationship with Ludwig Binswanger, the nature of his personality, and theoretical interests, particularly kinesthetic perception. A summary of phenomenological theory regarding Rorschach’s project is provided as well as potential future theoretical and methodological frameworks in light of this connection. Specific suggestions for Rorschach psychology, given solutions from historical and phenomenological explorations, are provided.
On the Background and Foundations of Psychodiagnostics
Authors: Silberstein Fernando
Published: October 2023

Abstract: During the century that has passed since its publication, there have been innumerable investigations on the Psychodiagnostics with relevant contributions in the codification, interpretation, and validity of its results. However, Rorschach’s work and his intentions remain in many respects little known. Knowledge of his research on the determinants and the foundations of their meanings is limited, although they are still valid and widely used. In this article the author studies the elaboration on the concepts of interpretation, the introduction of movement, Rorschach’s ideas about color, form, and Erlebnistypus as presented in Rorschach’s dissertation on reflex hallucinations and Psychodiagnostics.
A Tectonic Origin for the Largest Marsquake Observed by InSight
Authors: Fernando Benjamin , Daubar Ingrid J. , Charalambous Constantinos , Grindrod Peter M. , Stott Alexander , Al Ateqi Abdullah , Atri Dimitra , Ceylan Savas , Clinton John , Fillingim Matthew , Hauber Ernest , Hill Jonathon R. , Kawamura Taichi , Liu Jianjun , Lucas Antoine , Lorenz Ralph , Ojha Lujendra , Perrin Clement , Piqueux Sylvain , Stähler Simon , Tirsch Daniela , Wilson Colin , Wójcicka Natalia , Giardini Domenico , Lognonné Philippe , Banerdt W. Bruce
Published: October 2023

The S1222a marsquake detected by InSight on 4 May 2022 was the largest of the mission, at 4.7. Given its resemblance to two other large seismic events (S1000a and S1094b), which were associated with the formation of fresh craters, we undertook a search for a fresh crater associated with S1222a. Such a crater would be expected to be ∼300 m in diameter and have a blast zone on the order of 180 km across. Orbital images were targeted and searched as part of an international, multi‐mission effort. Comprehensive analysis of the area using low‐ and medium‐resolution images reveals no relevant transient atmospheric phenomena and no fresh blast zone. High‐resolution coverage of the epicentral area from most spacecraft are more limited, but no fresh crater or other evidence of a new impact have been identified in those images either. We thus conclude that the S1222a event was highly likely of tectonic origin.
Tropical Waves Are Key Drivers of Extreme Precipitation Events in the Central Sahel
Authors: Peyrillé Philippe , Roehrig Romain , Sanogo Sidiki
Published: October 2023

Extreme precipitation events (EPE) are often associated with severe floods and significant damages in Central Sahel. To better understand their formation and improve their forecasts, we investigate the sub‐seasonal drivers of EPEs. A composite analysis reveals that moist, cyclonic and upper‐level divergence anomalies are found on average as a result of several tropical waves. The equatorial Rossby wave (ER) dominates at large scale providing a moist and convectively‐active anomaly over the northern Sahel together with a smaller‐scale African Easterly Wave (AEW). The Madden‐Julian Oscillation provides upper‐level divergence anomalies and a Kelvin wave increases convection during the EPE. Statistics show the prevalence of AEW and emphasize ER as a key driver of EPE. The co‐occurrences of several tropical waves, especially those involving AEW, ER, and Kelvin waves, increase the probability of EPE. Monitoring these tropical waves combinations could improve EPEs forecasts.
Relativistic Runaway Electron Avalanche Development Near the Electric Field Threshold in Inhomogeneous Air
Authors: Diniz G. S. , Wada Y. , Ohira Y. , Nakazawa K. , Tsurumi M. , Enoto T.
Published: October 2023

Relativistic Runaway Electrons Avalanches (RREAs) development depends on the applied electric field and the environment's air density. This dependency controls the RREA exponential growth length scale. The RREA development affects the bremsstrahlung excess occurring due to the passage of charged particles through the thundercloud's electric fields, the gamma‐ray glow. We used Monte Carlo simulations to develop an empirical model showing the RREA behavior in a realistic atmospheric density profile. The new formulation shows how the density variation modulates the electron population under electric field strengths near the RREA electric field threshold. The model limits the initial RREA altitude range as a function of the electric field strength. The new model is valid between ∼0.6 and ∼18 km, covering the relevant heights to investigate the generation of ground‐detected gamma‐ray glows.
Linking Significant Zr Isotopic Fractionation in Magmatic Zircons With Petrographic Textures
Authors: Yuan Yu , Zong Keqing , Guo Jing‐Liang , Zhang Wen , Wang Zaicong , Moynier Frédéric , Feng Lanping , Hu Zhaochu , Liu Yongsheng
Published: October 2023

The origin of crystal‐scale Zr isotopic variation remains enigmatic. Here, we report high‐precision Zr isotopic compositions of thin‐sectioned and hand‐picked zircon crystals from monzodiorites and K‐feldspar granites, which represent the mafic and felsic products of a differentiating magma. Our results reveal that zircons from both rock associations are dominated by a rim‐ward increase in the 94Zr/90Zr ratio and accompanied by ∼1‰ intracrystalline and intercrystalline variations, confirming the preferential incorporation of light Zr isotopes and thus kinetic isotopic fractionation during zircon growth. Notably, zircons enclosed by K‐feldspar in monzodiorites generally yield lower 94Zr/90Zr and higher Zr/Hf ratios than that of separated crystals, while in K‐feldspar granites, tiny zircons intergrown with apatite obtain higher 94Zr/90Zr but lower Zr/Hf ratios relative to that of sizable separates. The diversity in petrographic textures and sizes of zircon crystals, corresponding to their crystallization sequence, potentially accounts for some of the heterogeneity of Zr isotopes at the mineral scale.
Green leaf volatile sensory calcium transduction in Arabidopsis
Authors: Aratani Yuri , Uemura Takuya , Hagihara Takuma , Matsui Kenji , Toyota Masatsugu
Published: October 2023

Plants perceive volatile organic compounds (VOCs) released by mechanically- or herbivore-damaged neighboring plants and induce various defense responses. Such interplant communication protects plants from environmental threats. However, the spatiotemporal dynamics of VOC sensory transduction in plants remain largely unknown. Using a wide-field real-time imaging method, we visualize an increase in cytosolic Ca2+ concentration ([Ca2+]cyt) in Arabidopsis leaves following exposure to VOCs emitted by injured plants. We identify two green leaf volatiles (GLVs), (Z)-3-hexenal (Z-3-HAL) and (E)-2-hexenal (E-2-HAL), which increase [Ca2+]cyt in Arabidopsis. These volatiles trigger the expression of biotic and abiotic stress-responsive genes in a Ca2+-dependent manner. Tissue-specific high-resolution Ca2+ imaging and stomatal mutant analysis reveal that [Ca2+]cyt increases instantly in guard cells and subsequently in mesophyll cells upon Z-3-HAL exposure. These results suggest that GLVs in the atmosphere are rapidly taken up by the inner tissues via stomata, leading to [Ca2+]cyt increases and subsequent defense responses in Arabidopsis leaves.
O-GlcNAcylation regulates neurofilament-light assembly and function and is perturbed by Charcot-Marie-Tooth disease mutations
Authors: Huynh Duc T. , Tsolova Kalina N. , Watson Abigail J. , Khal Sai Kwan , Green Jordan R. , Li Di , Hu Jimin , Soderblom Erik J. , Chi Jen-Tsan , Evans Chantell S. , Boyce Michael
Published: October 2023

The neurofilament (NF) cytoskeleton is critical for neuronal morphology and function. In particular, the neurofilament-light (NF-L) subunit is required for NF assembly in vivo and is mutated in subtypes of Charcot-Marie-Tooth (CMT) disease. NFs are highly dynamic, and the regulation of NF assembly state is incompletely understood. Here, we demonstrate that human NF-L is modified in a nutrient-sensitive manner by O-linked-β-N-acetylglucosamine (O-GlcNAc), a ubiquitous form of intracellular glycosylation. We identify five NF-L O-GlcNAc sites and show that they regulate NF assembly state. NF-L engages in O-GlcNAc-mediated protein-protein interactions with itself and with the NF component α-internexin, implying that O-GlcNAc may be a general regulator of NF architecture. We further show that NF-L O-GlcNAcylation is required for normal organelle trafficking in primary neurons. Finally, several CMT-causative NF-L mutants exhibit perturbed O-GlcNAc levels and resist the effects of O-GlcNAcylation on NF assembly state, suggesting a potential link between dysregulated O-GlcNAcylation and pathological NF aggregation. Our results demonstrate that site-specific glycosylation regulates NF-L assembly and function, and aberrant NF O-GlcNAcylation may contribute to CMT and other neurodegenerative disorders.
Dynamic emotional states shape the episodic structure of memory
Authors: McClay Mason , Sachs Matthew E. , Clewett David
Published: October 2023

Human emotions fluctuate over time. However, it is unclear how these shifting emotional states influence the organization of episodic memory. Here, we examine how emotion dynamics transform experiences into memorable events. Using custom musical pieces and a dynamic emotion-tracking tool to elicit and measure temporal fluctuations in felt valence and arousal, our results demonstrate that memory is organized around emotional states. While listening to music, fluctuations between different emotional valences bias temporal encoding process toward memory integration or separation. Whereas a large absolute or negative shift in valence helps segment memories into episodes, a positive emotional shift binds sequential representations together. Both discrete and dynamic shifts in music-evoked valence and arousal also enhance delayed item and temporal source memory for concurrent neutral items, signaling the beginning of new emotional events. These findings are in line with the idea that the rise and fall of emotions can sculpt unfolding experiences into memories of meaningful events.
Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Authors: Chou Chih-Wei , Hung Chia-Nung , Chiu Cheryl Hsiang-Ling , Tan Xi , Chen Meizhen , Chen Chien-Chin , Saeed Moawiz , Hsu Che-Wei , Liss Michael A. , Wang Chiou-Miin , Lai Zhao , Alvarez Nathaniel , Osmulski Pawel A. , Gaczynska Maria E. , Lin Li-Ling , Ortega Veronica , Kirma Nameer B. , Xu Kexin , Liu Zhijie , Kumar Addanki P. , Taverna Josephine A. , Velagaleti Gopalrao V. N. , Chen Chun-Liang , Zhang Zhao , Huang Tim Hui-Ming
Published: October 2023

While macrophage phagocytosis is an immune defense mechanism against invading cellular organisms, cancer cells expressing the CD47 ligand send forward signals to repel this engulfment. Here we report that the reverse signaling using CD47 as a receptor additionally enhances a pro-survival function of prostate cancer cells under phagocytic attack. Although low CD47-expressing cancer cells still allow phagocytosis, the reverse signaling delays the process, leading to incomplete digestion of the entrapped cells and subsequent tumor hybrid cell (THC) formation. Viable THCs acquire c-Myc from parental cancer cells to upregulate both M1- and M2-like macrophage polarization genes. Consequently, THCs imitating dual macrophage features can confound immunosurveillance, gaining survival advantage in the host. Furthermore, these cells intrinsically express low levels of androgen receptor and its targets, resembling an adenocarcinoma-immune subtype of metastatic castration-resistant prostate cancer. Therefore, phagocytosis-generated THCs may represent a potential target for treating the disease.
Atomic high-spin cobalt(II) center for highly selective electrochemical CO reduction to CH3OH
Authors: Ding Jie , Wei Zhiming , Li Fuhua , Zhang Jincheng , Zhang Qiao , Zhou Jing , Wang Weijue , Liu Yuhang , Zhang Zhen , Su Xiaozhi , Yang Runze , Liu Wei , Su Chenliang , Yang Hong Bin , Huang Yanqiang , Zhai Yueming , Liu Bin
Published: October 2023

In this work, via engineering the conformation of cobalt active center in cobalt phthalocyanine molecular catalyst, the catalytic efficiency of electrochemical carbon monoxide reduction to methanol can be dramatically tuned. Based on a collection of experimental investigations and density functional theory calculations, it reveals that the electron rearrangement of the Co 3d orbitals of cobalt phthalocyanine from the low-spin state (S = 1/2) to the high-spin state (S = 3/2), induced by molecular conformation change, is responsible for the greatly enhanced CO reduction reaction performance. Operando attenuated total reflectance surface-enhanced infrared absorption spectroscopy measurements disclose accelerated hydrogenation of CORR intermediates, and kinetic isotope effect validates expedited proton-feeding rate over cobalt phthalocyanine with high-spin state. Further natural population analysis and density functional theory calculations demonstrate that the high spin Co2+ can enhance the electron backdonation via the dxz/dyz−2π* bond and weaken the C-O bonding in *CO, promoting hydrogenation of CORR intermediates.
Thalamic nucleus reuniens coordinates prefrontal-hippocampal synchrony to suppress extinguished fear
Authors: Totty Michael S. , Tuna Tuğçe , Ramanathan Karthik R. , Jin Jingji , Peters Shaun E. , Maren Stephen
Published: October 2023

Traumatic events result in vivid and enduring fear memories. Suppressing the retrieval of these memories is central to behavioral therapies for pathological fear. The medial prefrontal cortex (mPFC) and hippocampus (HPC) have been implicated in retrieval suppression, but how mPFC-HPC activity is coordinated during extinction retrieval is unclear. Here we show that after extinction training, coherent theta oscillations (6–9 Hz) in the HPC and mPFC are correlated with the suppression of conditioned freezing in male and female rats. Inactivation of the nucleus reuniens (RE), a thalamic hub interconnecting the mPFC and HPC, reduces extinction-related Fos expression in both the mPFC and HPC, dampens mPFC-HPC theta coherence, and impairs extinction retrieval. Conversely, theta-paced optogenetic stimulation of RE augments fear suppression and reduces relapse of extinguished fear. Collectively, these results demonstrate a role for RE in coordinating mPFC-HPC interactions to suppress fear memories after extinction.
Windows of opportunity for predicting seasonal climate extremes highlighted by the Pakistan floods of 2022
Authors: Dunstone Nick , Smith Doug M. , Hardiman Steven C. , Davies Paul , Ineson Sarah , Jain Shipra , Kent Chris , Martin Gill , Scaife Adam A.
Published: October 2023

Skilful predictions of near-term climate extremes are key to a resilient society. However, standard methods of analysing seasonal forecasts are not optimised to identify the rarer and most impactful extremes. For example, standard tercile probability maps, used in real-time regional climate outlooks, failed to convey the extreme magnitude of summer 2022 Pakistan rainfall that was, in fact, widely predicted by seasonal forecasts. Here we argue that, in this case, a strong summer La Niña provided a window of opportunity to issue a much more confident forecast for extreme rainfall than average skill estimates would suggest. We explore ways of building forecast confidence via a physical understanding of dynamical mechanisms, perturbation experiments to isolate extreme drivers, and simple empirical relationships. We highlight the need for more detailed routine monitoring of forecasts, with improved tools, to identify regional climate extremes and hence utilise windows of opportunity to issue trustworthy and actionable early warnings.
Intercalation-type catalyst for non-aqueous room temperature sodium-sulfur batteries
Authors: He Jiarui , Bhargav Amruth , Su Laisuo , Charalambous Harry , Manthiram Arumugam
Published: October 2023

Ambient-temperature sodium-sulfur (Na-S) batteries are potential attractive alternatives to lithium-ion batteries owing to their high theoretical specific energy of 1,274 Wh kg−1 based on the mass of Na2S and abundant sulfur resources. However, their practical viability is impeded by sodium polysulfide shuttling. Here, we report an intercalation-conversion hybrid positive electrode material by coupling the intercalation-type catalyst, MoTe2, with the conversion-type active material, sulfur. In addition, MoTe2 nanosheets vertically grown on graphene flakes offer abundant active catalytic sites, further boosting the catalytic activity for sulfur redox. When used as a composite positive electrode and assembled in a coin cell with excess Na, a discharge capacity of 1,081 mA h gs−1 based on the mass of S with a capacity fade rate of 0.05% per cycle over 350 cycles at 0.1 C rate in a voltage range of 0.8 to 2.8 V is realized under a high sulfur loading of 3.5 mg cm−2 and a lean electrolyte condition with an electrolyte-to-sulfur ratio of 7 μL mg−1. A fundamental understanding of the electrocatalysis of MoTe2 is further revealed by in-situ synchrotron-based operando X-ray diffraction and ex-situ time-of-flight secondary ion mass spectrometry.
Post-interventional infectious complications in percutaneous transabdominal lymphatic interventions: an observational study
Authors: Pieper Claus Christian , Geiger Sergej , Kupczyk Patrick , Luetkens Julian A. , Köster Thomas , Attenberger Ulrike I. , Schild Hans Heinz
Published: October 2023

The purpose of this retrospective study was to evaluate the occurrence of infectious complications and inflammatory reactions after transabdominal lymphatic-interventions. 63 lymphatic-interventions were performed in 60 patients (male/female: 35/25; mean age 56 [9–85] years) [chylothorax n = 48, chylous ascites n = 7, combined chylothorax/chylous ascites n = 5]. Post-interventional clinical course and laboratory findings were analyzed in the whole cohort as well as subgroups without (group A; n = 35) and with peri-interventional antibiotics (group B; n = 25) (pneumonia n = 16, drainage-catheter inflammation n = 5, colitis n = 1, cystitis n = 1, transcolonic-access n = 2). No septic complications associated with the intervention occurred. Leucocytes increased significantly, peaking on post-interventional day-1 (8.6 ± 3.9 × 106 cells/mL vs. 9.8 ± 4.7 × 106 cells/mL; p = 0.009) and decreased thereafter (day-10: 7.3 ± 2.7 × 106 cells/mL, p = 0.005). CRP-values were pathological in 89.5% of patients already at baseline (40.1 ± 63.9 mg/L) and increased significant on day-3 (77.0 ± 78.8 mg/L, p < 0.001). Values decreased thereafter (day-15: 25.3 ± 34.4 mg/L, p = 0.04). In subgroup B, 13/25 patients had febrile episodes post-interventionally (pneumonia n = 11, cystitis n = 1, drainage-catheter inflammation n = 1). One patient developed biliary peritonitis despite continued antibiotics and underwent cholecystectomy. Baseline leucocytes and CRP-levels were higher in group B than A, but with comparable post-interventional profiles. Clinically relevant infectious complications associated with transabdominal lymphatic-interventions are rare irrespective of peri-interventional antibiotic use. Post-interventional elevation of leucocytes and CRP are observed with normalization over 10–15 days.
A novel role for the chloride intracellular channel protein Clic5 in ciliary function
Authors: Ott Elisabeth , Hoff Sylvia , Indorf Lara , Ditengou Franck Anicet , Müller Julius , Renschler Gina , Lienkamp Soeren S. , Kramer-Zucker Albrecht , Bergmann Carsten , Epting Daniel
Published: October 2023

CLIC5 belongs to a family of ion channels with six members reported so far. In vertebrates, the CLIC5 gene encodes two different isoforms, CLIC5A and CLIC5B. In addition to its ion channel activity, there is evidence for further functions of CLIC5A, such as the remodeling of the actin cytoskeleton during the formation of a functional glomerulus in the vertebrate kidney. However, its specific role is still incompletely understood and a specific functional role for CLIC5B has not been described yet. Here we report our findings on the differential expression and functions of Clic5a and Clic5b during zebrafish kidney development. Whole-mount in situ hybridization studies revealed specific expression of clic5a in the eye and pronephric glomerulus, and clic5b is expressed in the gut, liver and the pronephric tubules. Clic5 immunostainings revealed that Clic5b is localized in the cilia. Whereas knockdown of Clic5a resulted in leakiness of the glomerular filtration barrier, Clic5b deficient embryos displayed defective ciliogenesis, leading to ciliopathy-associated phenotypes such as ventral body curvature, otolith deposition defects, altered left–right asymmetry and formation of hydrocephalus and pronephric cysts. In addition, Clic5 deficiency resulted in dysregulation of cilia-dependent Wnt signalling pathway components. Mechanistically, we identified a Clic5-dependent activation of the membrane-cytoskeletal linker proteins Ezrin/Radixin/Moesin (ERM) in the pronephric tubules of zebrafish. In conclusion, our in vivo data demonstrates a novel role for Clic5 in regulating essential ciliary functions and identified Clic5 as a positive regulator of ERM phosphorylation.
The effect of Trichoderma harzianum agents on physiological-biochemical characteristics of cucumber and the control effect against Fusarium wilt
Authors: Lian Hua , Li Runzhe , Ma Guangshu , Zhao Zhenghan , Zhang Ting , Li Mei
Published: October 2023

At the seedling and adult plant phases, pot experiments were carried out to enhance the physiological-biochemical characteristics of cucumber, guarantee its high yield, and ensure its cultivation of quality. Trichoderma harzianum conidia agents at 104, 105, 106, and 107 cfu g−1 were applied in accordance with the application of Fusarium oxysporum powder at concentrations of 104 cfu/g on the protective enzyme activity, physiological and biochemical indices, seedling quality, resilience to Fusarium wilt, quality, and yield traits. Fusarium oxysporum powder at 104 cfu g−1 was used to treat CK1, while Fusarium oxysporum powder and T. harzianum conidia agents were not used to treat CK2. The results show that different T. harzianum agents improved the activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), and peroxidase (POD) in cucumber seedlings, improved chlorophyll content, root activity, root-shoot ratio, and seedling strength index, and decreased malondialdehyde (MAD) content (P < 0.05). T3, a combination of 104 cfu g−1 Fusarium oxysporum powder and 106 cfu g−1 T. harzianum conidia agents, had the greatest promoting effect. The effects of different T. harzianum conidia agents and their application amounts on the control of cucumber Fusarium wilt were explored. T3 had the best promotion impact, and the control effect of cucumber Fusarium wilt at seedling stage and adult stage reached 83.98% and 70.08%, respectively. The quality index and yield formation of cucumber were also increased by several T. harzianum agents, with T3 having the strongest promotion effects. In comparison to CK1, the soluble sugar, Vc, soluble protein, and soluble solid contents of T3 cucumber fruit were 120.75%, 39.14%, 42.26%, and 11.64% higher (P < 0.05), respectively. In comparison to CK2, the soluble sugar, Vc, soluble protein, and soluble solid contents of T3 cucumber fruit were 66.06%, 24.28%, 36.15%, and 7.95% higher (P < 0.05), respectively. In comparison to CK1 and CK2, the yields of T3 cucumber were 50.19% and 35.86% higher, respectively. As a result, T. harzianum agents can enhance the physiological and biochemical traits of cucumber seedlings, raise the quality of cucumber seedlings, have a controlling impact on Fusarium wilt, and increase the yield and quality of cucumber fruit. The greatest effectiveness of T3 comes from its use. In this study, Trichoderma harzianum conidia agents demonstrated good impacts on cucumber yield formation and plant disease prevention, demonstrating their high potential as biocontrol agents.
Acute stress impacts reaction times in older but not in young adults in a flanker task
Authors: Mikneviciute Greta , Allaert Jens , Pulopulos Matias M. , De Raedt Rudi , Kliegel Matthias , Ballhausen Nicola
Published: October 2023

Acute psychosocial stress effects on inhibition have been investigated in young adults, but little is known about these effects in older adults. The present study investigated effects of the Trier Social Stress Test on cognitive inhibition (i.e., ability to ignore distracting information) using a cross-over (stress vs. control) design in healthy young (N = 50; 18–30 years; Mage = 23.06) versus older adults (N = 50; 65–84 years; Mage = 71.12). Cognitive inhibition was measured by a letter flanker task and psychophysiological measures (cortisol, heart rate, subjective stress) validated the stress induction. The results showed that while stress impaired overall accuracy across age groups and sessions, stress (vs. control) made older adults’ faster in session 1 and slower in session 2. Given that session 2 effects were likely confounded by practice effects, these results suggest that acute psychosocial stress improved older adults’ RTs on a novel flanker task but impaired RTs on a practiced flanker task. That is, the interaction between stress and learning effects might negatively affect response execution when testing older adults on flanker tasks. If confirmed by future research, these results might have important implications especially in settings where repeated cognitive testing is performed under acute stress.
Continuous shear wave measurements for dynamic cardiac stiffness evaluation in pigs
Authors: Caenen Annette , Keijzer Lana , Bézy Stéphanie , Duchenne Jürgen , Orlowska Marta , Van Der Steen Antonius F. W. , De Jong Nico , Bosch Johan G. , Voigt Jens-Uwe , D’hooge Jan , Vos Hendrik J.
Published: October 2023

Ultrasound-based shear wave elastography is a promising technique to non-invasively assess the dynamic stiffness variations of the heart. The technique is based on tracking the propagation of acoustically induced shear waves in the myocardium of which the propagation speed is linked to tissue stiffness. This measurement is repeated multiple times across the cardiac cycle to assess the natural variations in wave propagation speed. The interpretation of these measurements remains however complex, as factors such as loading and contractility affect wave propagation. We therefore applied transthoracic shear wave elastography in 13 pigs to investigate the dependencies of wave speed on pressure–volume derived indices of loading, myocardial stiffness, and contractility, while altering loading and inducing myocardial ischemia/reperfusion injury. Our results show that diastolic wave speed correlates to a pressure–volume derived index of operational myocardial stiffness (R = 0.75, p < 0.001), suggesting that both loading and intrinsic properties can affect diastolic wave speed. Additionally, the wave speed ratio, i.e. the ratio of systolic and diastolic speed, correlates to a pressure–volume derived index of contractility, i.e. preload-recruitable stroke work (R = 0.67, p < 0.001). Measuring wave speed ratio might thus provide a non-invasive index of contractility during ischemia/reperfusion injury.
Effect of the German tonsillitis guideline on indication for tonsil surgery in patients with recurrent acute tonsillitis: a population-based study
Authors: Betz Valerie , Boeger Daniel , Buentzel Jens , Hoffmann Kerstin , Jecker Peter , Kaftan Holger , Mueller Andreas , Radtke Gerald , Geißler Katharina , Guntinas-Lichius Orlando
Published: October 2023

Evidence-based indication for tonsil surgery in patients with recurrent acute tonsillitis (RAT) is an ongoing matter of debate. Since introduction of the German tonsillitis guideline in 2015, the indication criteria for tonsil surgery have become much stricter. It is unclear, if this has changed the indication policy. A retrospective population-based study was performed including all 1398 patients with RAT admitted for tonsil surgery in all Thuringian hospitals in 2011, 2015, and 2019. Changes over the years concerning patients’ characteristics, number of tonsillitis episodes in the last 12 months treated with antibiotics (T12), and decision for tonsillectomy or tonsillotomy were analyzed using univariable and multivariable statistics. The surgical rates decreased from 28.56/100,000 population in 2011 to 23.57 in 2015, and to 11.60 in 2019. The relative amount of patients with ≥ 6 T12 increased from 14.1% in 2011 over 13.3% in 2015 to 35.9% in 2019. Most patients received a tonsillectomy (98% of all surgeries). Decision for tonsillotomy was seldom (1.2%). Multinomial logistic regression analysis with the year 2011 as reference showed that compared to the year 2015, the age of the patients undergoing surgery increased in 2015 (Odds ratio [OR] = 1.024; 95% confidence interval [CI] = 1.014–1.034; p < 0.001), and also in 2019 (OR 1.030: CI 1.017–1.043; p < 0.001). Compared to 2011, the number T12 was not higher in 2015, but in 2019 (OR 1.273; CI 1.185–1.367; p < 0.001). Stricter rules led to lower tonsil surgery rates but to a higher proportion of patients with ≥ 6 T12 before surgery. Tonsillectomy remained the dominating technique.
Urinary tract infections and intermittent catheterization among patients with spinal cord injury in Chinese community
Authors: Liu Jiawei , Luo Can , Xiao Weichu , Xu Tao
Published: October 2023

We conducted a cross-sectional study using an online questionnaire to investigate the current status of urinary tract infections (UTIs) and the use of clean intermittent catheterization (CIC) in Chinese community-based SCI patients and to explore the risk factors for UTIs in patients using CIC. Our findings suggest that the prevalence of UTIS is higher in Chinese community-based SCI patients compared with patients in medically developed countries. In addition, we found that CIC had the lower incidence of UTIs than urinary indwelling catheter (UIC) and suprapubic catheter (SPC), and that SCI patients with CIC had low rates of use and poor compliance. Further analysis indicated that most of the risk factors for UTIs in CIC patients were associated with irregular use of CIC. Therefore, we call for not only the need to provide stronger caregiver support and financial assistance to improve CIC adherence in Chinese community SCI patients, but also the establishment of a database of Chinese SCI patients in order to enhance the management of bladder emptying methods and further standardize the CIC operation in such patients, thus reducing the risk of UTIs in Chinese community SCI patients.
Multi-ancestry epigenome-wide analyses identify methylated sites associated with aortic augmentation index in TOPMed MESA
Authors: Hu Xiaowei , Logan Jeongok G. , Kwon Younghoon , Lima Joao A. C. , Jacobs David R. , Duprez Daniel , Brumback Lyndia , Taylor Kent D. , Durda Peter , Johnson W. Craig , Cornell Elaine , Guo Xiuqing , Liu Yongmei , Tracy Russell P. , Blackwell Thomas W. , Papanicolaou George , Mitchell Gary F. , Rich Stephen S. , Rotter Jerome I. , Van Den Berg David J. , Chirinos Julio A. , Hughes Timothy M. , Garrett-Bakelman Francine E. , Manichaikul Ani
Published: October 2023

Despite the prognostic value of arterial stiffness (AS) and pulsatile hemodynamics (PH) for cardiovascular morbidity and mortality, epigenetic modifications that contribute to AS/PH remain unknown. To gain a better understanding of the link between epigenetics (DNA methylation) and AS/PH, we examined the relationship of eight measures of AS/PH with CpG sites and co-methylated regions using multi-ancestry participants from Trans-Omics for Precision Medicine (TOPMed) Multi-Ethnic Study of Atherosclerosis (MESA) with sample sizes ranging from 438 to 874. Epigenome-wide association analysis identified one genome-wide significant CpG (cg20711926-CYP1B1) associated with aortic augmentation index (AIx). Follow-up analyses, including gene set enrichment analysis, expression quantitative trait methylation analysis, and functional enrichment analysis on differentially methylated positions and regions, further prioritized three CpGs and their annotated genes (cg23800023-ETS1, cg08426368-TGFB3, and cg17350632-HLA-DPB1) for AIx. Among these, ETS1 and TGFB3 have been previously prioritized as candidate genes. Furthermore, both ETS1 and HLA-DPB1 have significant tissue correlations between Whole Blood and Aorta in GTEx, which suggests ETS1 and HLA-DPB1 could be potential biomarkers in understanding pathophysiology of AS/PH. Overall, our findings support the possible role of epigenetic regulation via DNA methylation of specific genes associated with AIx as well as identifying potential targets for regulation of AS/PH.
Covalently anchoring silver nanoclusters Ag44 on modified UiO-66-NH2 with Bi2S3 nanorods and MoS2 nanoparticles for exceptional solar wastewater treatment activity
Authors: Farrag Mostafa
Published: October 2023

For the first time, covalently anchoring size selected silver nanoclusters [Ag44(MNBA)30] on the Bi2S3@UiO-66-NH2 and MoS2@UiO-66-NH2 heterojunctions were constructed as novel photocatalysts for photodegradation of methylene blue (MB) dye. The anchoring of Ag44 on MoS2@UiO-66-NH2 and Bi2S3@UiO-66-NH2 heterojunctions extended the light absorption of UiO-66-NH2 to the visible region and improved the transfer and separation of photogenerated charge carriers through the heterojunctions with a unique band gap structure. The UV–Vis-NIR diffuse reflectance spectroscopic analysis confirmed that the optical absorption properties of the UiO-66-NH2 were shifted from the UV region at 379 nm to the visible region at ~ 705 nm after its doping with Bi2S3 nanorods and Ag44 nanoclusters (Bi2S3@UiO-66-NH-S-Ag44). The prepared Bi2S3@UiO-66-NH-S-Ag44 and MoS2@UiO-66-NH-S-Ag44 photocatalysts exhibited exceptional photocatalytic activity for visible light degradation of MB dye. The photocatalysts exhibited complete decolorization of the MB solution (50 ppm) within 90 and 120 min stirring under visible light irradiation, respectively. The supper photocatalytic performance and recycling efficiency of the prepared photocatalysts attributed to the covalent anchoring of the ultra-small silver clusters (Ag44) on the heterojunctions surface. The X-ray photoelectron spectroscopic analysis confirmed the charge of the silver clusters is zero. The disappearance of the N–H bending vibration peak of primary amines in the FTIR analysis of Bi2S3@UiO-66-NH-S-Ag44 confirmed the covalent anchoring of the protected silver nanoclusters on the UiO-66-NH2 surface via the condensation reaction. The Bi2S3@UiO-66-NH-S-Ag44 catalyst exhibited excellent recyclability efficiency more than five cycles without significant loss in activity, indicating their good potential for industrial applications. The texture properties, crystallinity, phase composition, particle size, and structural morphology of the prepared photocatalysts were investigated using adsorption–desorption N2 isotherms, X-ray diffraction (XRD), HR-TEM, and FE-SEM, respectively.
Green UPLC method for estimation of ciprofloxacin, diclofenac sodium, and ibuprofen with application to pharmacokinetic study of human samples
Authors: Ahmed-Anwar Alaa A. , Mohamed Mahmoud A. , Farghali Ahmed A. , Mahmoud Rehab , Hassouna Mohamed E. M.
Published: October 2023

Investigation of a unique and fast method for the determination and separation of a mixture of three drugs viz., ciprofloxacin (CIP), Ibuprofen (IBU), and diclofenac sodium (DIC) in actual samples of human plasma. Also, the technique was used to look at their pharmacokinetics study. Hydrocortisone was chosen as the internal standard (IS). The drugs were chromatographically separated using an Acquity ultra-performance liquid chromatography UPLC ® BEH C18 1.7 µm (2.1 × 150 mm) column with a mobile phase composed of acetonitrile: water (65:35, v/v) adjusted to pH 3 with diluted acetic acid. Plasma proteins were precipitated with acetonitrile. The separated drugs ranged from 0.3 to 10, 0.2–11, and 1–25 µg/mL for CIP, IBU, and DIC, respectively. Calibration curves were discovered to achieve linearity with acceptable correlation coefficients (0.99%). Examination of quality assurance samples showed exceptional precision and accuracy. Following the successful application of this improved technique to plasma samples, the pharmacokinetic characteristics of each selected drug were evaluated using (UPLC) with UV detection at 210 nm. Two green metrics were applied, the Analytical Eco-scale and the Analytical GREEnness Calculator (AGREE). Separation was achieved in only 4-min analysis time. The method's validation agreed with the requirements of the FDA, and the results were sufficient.
A comprehensive review of viscoelastic polymer flooding in sandstone and carbonate rocks
Authors: Zeynalli Mursal , Mushtaq Muhammad , Al-Shalabi Emad W. , Alfazazi Umar , Hassan Anas M. , AlAmeri Waleed
Published: October 2023

Polymer flooding is a proven chemical Enhanced Oil Recovery (cEOR) method that boosts oil production beyond waterflooding. Thorough theoretical and practical knowledge has been obtained for this technique through numerous experimental, simulation, and field works. According to the conventional belief, this technique improves macroscopic sweep efficiency due to high polymer viscosity by producing moveable oil that remains unswept after secondary recovery. However, recent studies show that in addition to viscosity, polymer viscoelasticity can be effectively utilized to increase oil recovery by mobilizing residual oil and improving microscopic displacement efficiency in addition to macroscopic sweep efficiency. The polymer flooding is frequently implemented in sandstones with limited application in carbonates. This limitation is associated with extreme reservoir conditions, such as high concentrations of monovalent and divalent ions in the formation brine and ultimate reservoir temperatures. Other complications include the high heterogeneity of tight carbonates and their mixed-to-oil wettability. To overcome the challenges related to severe reservoir conditions, novel polymers have been introduced. These new polymers have unique monomers protecting them from chemical and thermal degradations. Monomers, such as NVP (N-vinylpyrrolidone) and ATBS (2-acrylamido-2-methylpropane sulfonic acid), enhance the chemical resistance of polymers against hydrolysis, mitigating the risk of viscosity reduction or precipitation in challenging reservoir conditions. However, the viscoelasticity of these novel polymers and their corresponding impact on microscopic displacement efficiency are not well established and require further investigation in this area. In this study, we comprehensively review recent works on viscoelastic polymer flow under various reservoir conditions, including carbonates and sandstones. In addition, the paper defines various mechanisms underlying incremental oil recovery by viscoelastic polymers and extensively describes the means of controlling and improving their viscoelasticity. Furthermore, the polymer screening studies for harsh reservoir conditions are also included. Finally, the impact of viscoelastic synthetic polymers on oil mobilization, the difficulties faced during this cEOR process, and the list of field applications in carbonates and sandstones can also be found in our work. This paper may serve as a guide for commencing or performing laboratory- and field-scale projects related to viscoelastic polymer flooding.