bluuebunny/crossref_metadata_2025 huggingface dataset:
An empowerment-based, healthy dietary behavioral intervention to ameliorate functional constipation
Authors: Wang Xuesong , Zhong Xiaohui , Liu Dongsong , Cao Hong , Chen Jing , Wang Qinyue , Xia Yanping , Zhang Feng
Published: March 2023

Objective

To explore the boost effect on ameliorating functional constipation in elderly patients through empowerment-based, healthy dietary behavioral intervention.

Design

In this randomized parallel group study, elderly patients with functional constipation were recruited and assigned to the experimental and control groups at a ratio of 1:1. The control group received routine intervention. The experimental group received 3-month empowerment-based intervention. The results were evaluated based on the Healthy Lifestyle and Personal Control Questionnaire (HLPCQ) and Cleveland Clinic Constipation Score (CCS). GraphPad Prism (Version 9) software was used for the statistical analysis.

Setting

As the world's population ages, functional constipation in the elderly has attracted widespread attention. The practical behavioral intervention to ameliorate constipation are worth exploring.

Participants

Sixty elderly patients with functional constipation.

Results

The study results showed no significant difference in the baseline data between the two groups (P > 0.05). After the intervention, the scores of HLPCQ (77.90 ± 14.57 vs. 61.11 ± 13.64) and CCS (7.48 ± 3.73 vs. 9.70 ± 3.07) in the experimental group were significantly higher than those in the control group (P < 0.05).

Conclusion

The results showed that empowerment-based intervention can effectively strengthen the healthy dietary behavior of elderly patients. Through patient empowerment, the subjective initiative and willingness to communicate were boosted in the experimental group. Their symptoms of functional constipation improved considerably better than in the control group.
ERAS with or without supplemental artificial nutrition in open pancreatoduodenectomy for cancer. A multicenter, randomized, open labeled trial (RASTA study protocol)
Authors: Gianotti Luca , Paiella Salvatore , Frigerio Isabella , Pecorelli Nicolò , Capretti Giovanni , Sandini Marta , Bernasconi Davide Paolo
Published: March 2023

Purpose

The role of supplemental artificial nutrition in patients perioperatively treated according to enhanced recovery programs (ERAS) on surgery-related morbidity is not known. Therefore, there is a need of a clinical trials specifically designed to explore whether given a full nutritional requirement by parenteral feeding after surgery coupled with oral food “at will” compared to oral food “at will” alone, within an established ERAS program, could achieve a reduction of the morbidity burden.

Materials and analysis

RASTA will be a multicenter, randomized, parallel-arm, open labeled, superiority trial. The trial will be conducted in five Italian Institutions with proven experience in pancreatic surgery and already applying an established ERAS program. Adult patients (age ≥ 18 and < 90 years of age) candidate to elective open pancreatoduodenectomy (PD) for any periampullary or pancreatic cancer will be randomized to receive a full ERAS protocol that establishes oral food “at will” plus parenteral nutrition (PN) from postoperative day 1 to day 5 (treatment arm), or to ERAS protocol without PN (control arm). The primary endpoint of the trial is the complication burden within 90 days after the day of surgery. The complication burden will be assessed by the Comprehensive Complication Index, that incorporates all complications and their severity as defined by the Clavien-Dindo classification, and summarizes postoperative morbidity with a numerical scale ranging from 0 to 100. The H0 hypothesis tested is that he administration of a parenteral nutrition added to the ERAS protocol will not affect the CCI as compared to standard of care (ERAS). The H1 hypothesis is that the administration of a parenteral nutrition added to the ERAS protocol will positively affect the CCI as compared to standard of care (ERAS). The trial has been registered at ClinicalTrials.gov (number: NCT04438447; date: 18/05/2020).

Conclusion

This upcoming trial will permit to establish if early postoperative artificial nutritional support after PD may improve postoperative outcomes compared to oral nutrition alone within an established ERAS program.
Characteristics and properties of a polysaccharide isolated from Wolfiporia cocos as potential dietary supplement for IBS
Authors: Yang Xuan , Lu Shun , Feng Yuhan , Cao Chongjiang , Zhang Yanliang , Cheng Shujie
Published: March 2023

Introduction

As low FODMAP (Fermentable oligosaccharides, disaccharides, monosaccharides and polyols) diet therapy is recommended for most of Irritable Bowel Syndrome (IBS) patients, the consequent insufficient of dietary fibers (DFs) intake exert an adverse impact on intestinal health. It is necessary to find suitable DFs for IBS patients.

Methods

This study extracted a water-insoluble polysaccharide from Wolfiporia cocos (WIP) by alkali-extraction and acid-precipitation method. Its molecular weight was detected by high performance gel permeation chromatography (HPGPC) analysis. The structure of WIP was analyzed by Fourier transform infrared (FT-IR) spectrum, Nuclear Magnetic Resonance (NMR) spectra and X-ray diffraction (XRD). The properties related to stability, digestion, viscosity, osmotic activity, adsorption and fermentation were investigated, aimed to explore the feasibility of WIP as a new DF supplement for patients with IBS. In addition, 16S rRNA sequencing analysis was conducted to explore its effects on IBS-related gut microbiota.

Results and Discussion

The results showed that WIP had a single homogeneous composition and the molecular weight was 8.1 × 103 Da. WIP was indicated as a kind of pyranose form with β anomeric configuration and the main chain of WIP was 1,3-β-glucan with amorphous structure. In addition to good thermal stability, WIP also has low bioavailability and can reach the colon mostly without being digested. Moreover, the low viscosity and osmotic activity, the high water- swelling and water/oil-holding capacity, fructose adsorption capacity and poor fermentation performance of WIP demonstrated that it is suitable for IBS patients. It is worth noting that WIP regulates IBS associated gut microbiota effectively, such as the abundance of Lachnospiraceae and Prevotella. These findings provide a theoretical basis for the development of WIP as a dietary supplement for IBS patients with low FODMAP diet therapy.

GRAPHICAL ABSTRACT
US consumers’ mental associations with meat substitute products
Authors: Garaus Marion , Garaus Christian
Published: March 2023

Negative impacts of meat consumption on both consumers’ health and the environment call for alternative sources for protein intake. In the last decades, the development of meat substitute products has made enormous progress. Given the beneficial aspects of reduced meat consumption, meat substitutes might be a promising approach for a more plant-based diet. However, despite the continuous improvement of meat substitute products and their increasing market potential, meat consumption in the US is still at a high level. Extant literature acknowledges that meat substitute products prompt several negative thoughts and feelings in various European countries, while US consumers’ perceptions of meat substitute products have not been investigated so far. However, understanding consumers’ thoughts and feelings toward meat substitute products provides valuable insights which can help policymakers and marketers to efficiently promote meat substitute products. Against this background, the current research investigates US consumers’ mental associations (i.e., connections of information and prior experiences with the product category stored in memory) with meat substitute products and explores if there are any differences between women and men. A sample of 175 US citizens acquired through an online panel provider completed a free word association technique resulting in 824 mental associations that qualified for the subsequent analysis. In a deductive-inductive content analysis, we assigned the mental associations to 20 categories (e.g., taste, health, environment) and determined their valence (i.e., positive, neutral, or negative). Frequencies and relationships among the categories were analyzed by employing frequency analyses, Chi-square difference tests, and multidimensional correspondence analysis. The findings reveal that meat substitute products elicit more negative mental associations than positive ones. Results validate categories identified in existing literature, but also reveal new categories of mental associations. Furthermore, the findings demonstrate that mental associations differ between women and men, with women tending to perceive meat substitutes more negatively than men. The multiple correspondence analysis resulted in four different consumer profiles (skeptics, innovators, health-oriented consumers, and avoiders) which can guide policymakers and brand managers on the effective promotion of meat substitute products.
Structural characterization and anti-inflammatory activity of a novel polysaccharide PKP2-1 from Polygonatum kingianum
Authors: Wang Zhen , Liu Hui , Fu Ranze , Ou Jinmei , Wang Bin
Published: March 2023

Introduction

This study aimed to investigate the structure characterization and antiinflammatory activity of a novel polysaccharide, PKP2-1, from the rhizomes of Polygonatum kingianum Coll. and Hemsl.

Methods

We isolated a novel polysaccharide, PKP2-1, from the rhizomes of Polygonatum kingianum Coll. and Hemsl. for the first time, which was then successively purified through hot-water extraction, 80% alcohol precipitation, anion exchange and gel permeation chromatography. The in vitro anti-inflammatory activity of PKP2-1 in MH7A cells was assessed using a CCK-8 kit assay.

Results

Monosaccharide composition assay revealed that PKP2-1 was mainly composed of glucose, galactose, mannose, and glucuronic acid at an approximate molar ratio of 6:2:2:1. It had a molecular weight of approximately 17.34 kDa. Structural investigation revealed that the backbone of PKP2-1 consisted of (→2, 3)-α-D-Galp(4→, →2)-α-D-Manp(3→, →2)-β-D-Glcp(4→) and α-D-Glcp(3→) residues with side chains (→2)-β-D-Glcp(4→, →1)-α-D-Galp(4→) and α-D-Glcp(3→) branches located at O-3 position of (→2, 3)-α-D-Galp(4→). The in vitro anti-inflammatory activity of PKP2-1 in MH7A cells revealed that PKP2-1 could reduce the expression of IL-11β and IL-6, increase the expression of IL-10 and induce apoptosis of synovial fibroblasts.

Conclusion

The PKP2-1 could inhibit MH7A cell growth and potentially be exploited as an anti-inflammatory agent.
KRAS-specific antibody binds to KRAS protein inside colorectal adenocarcinoma cells and inhibits its localization to the plasma membrane
Authors: Lam Kuen Kuen , Low Yee Syuen , Lo Michelle , Wong Michelle , Leong Tang Choong , Tan Emile , Chok Aik Yong , Seow-En Isaac , Wong Siew Heng , Cheah Peh Yean
Published: March 2023

Colorectal cancer (CRC) is the third highest incidence cancer and a leading cause of cancer mortality worldwide. To date, chemotherapeutic treatment of advanced CRC that has metastasized has a dismayed success rate of less than 30%. Further, most (80%) sporadic CRCs are microsatellite-stable and are refractory to immune checkpoint blockade therapy. KRAS is a gatekeeper gene in colorectal tumorigenesis. Nevertheless, KRAS is ‘undruggable’ due to its structure. Thus, focus has been diverted to develop small molecule inhibitors for its downstream effector such as ERK/MAPK. Despite intense research efforts for the past few decades, no small molecule inhibitor has been in clinical use for CRC. Antibody targeting KRAS itself is an attractive alternative. We developed a transient ex vivo patient-derived matched mucosa-tumor primary culture to assess whether anti-KRAS antibody can be internalized to bind and inactivate KRAS. We showed that anti-KRAS antibody can enter live mucosa-tumor cells and specifically aggregate KRAS in the cytoplasm, thus hindering its translocation to the inner plasma membrane. The mis-localization of KRAS reduces KRAS dwelling time at the site where it tethers to activate downstream effectors. We previously showed that expression of SOX9 was KRAS-mutation-dependent and possibly a better effector than ERK in CRC. Herein, we showed that anti-KRAS antibody treated tumor cells have less intense SOX9 cytoplasmic and nuclear staining compared to untreated cells. Our results demonstrated that internalized anti-KRAS antibody inhibits KRAS function in tumor. With an efficient intracellular antibody delivery system, this can be further developed as combinatorial therapeutics for CRC and other KRAS-driven cancers.
A pyroptosis-related gene signature for prognosis prediction in hepatocellular carcinoma
Authors: Chen Yongwei , Zhu Yanyun , Dong Yuanmei , Li Huizi , Gao Chumeng , Zhu Guoqiang , Mi Xiao , Li Chengcheng , Xu Yu , Wang Guoqiang , Cai Shangli , Han Yusheng , Xu Chunwei , Wang Wenxian , Yang Shizhong , Ji Wenbin
Published: March 2023

Introduction

Hepatocellular carcinoma (HCC) is one of the most invasive cancers with a low 5-year survival rate. Pyroptosis, a specialized form of cell death, has shown its association with cancer progression. However, its role in the prognosis of HCC has not been fully understood.

Methods

In our study, clinical information and mRNA expression for 1076 patients with HCC were obtained from the five public cohorts. Pyroptotic clusters were generated by unsupervised clustering based on 40 pyroptosis-related genes (PRGs) in the TCGA and ICGC cohort. A pyroptosis-related signature was constructed using least absolute shrinkage and selection operator (LASSO) regression according to differentially expressed genes (DEGs) of pyroptotic clusters. The signature was then tested in the validation cohorts (GES10142 and GSE14520) and subsequently validated in the CPTAC cohort (n=159) at both mRNA and protein levels. Response to sorafenib was explored in GSE109211.

Results

Three clusters were identified based on the 40 PRGs in the TCGA cohort. A total of 24 genes were selected based on DEGs of the above three pyroptotic clusters to construct the pyroptotic risk score. Patients with the high-risk score showed shorter overall survival (OS) compared to those with the low-risk score in the training set (P<0.001; HR, 3.06; 95% CI, 2.22-4.24) and the test set (P=0.008; HR, 1.61; 95% CI, 1.13-2.28). The predictive ability of the risk score was further confirmed in the CPTAC cohort at both mRNAs (P<0.001; HR, 2.99; 95% CI, 1.67-5.36) and protein levels (P<0.001; HR, 2.97; 95% CI 1.66-5.31). The expression of the model genes was correlated with immune cell infiltration, angiogenesis-related genes, and sensitivity to antiangiogenic therapy (P<0.05).

Discussion

In conclusion, we established a prognostic signature of 24 genes based on pyroptosis clusters for HCC patients, providing insight into the risk stratification of HCC.
Development and validation of a unifying pre-treatment decision tool for intracranial and extracranial metastasis-directed radiotherapy
Authors: Kowalchuk Roman , Mullikin Trey C. , Breen William , Gits Hunter C. , Florez Marcus , De Brian , Harmsen William S. , Rose Peter Sean , Siontis Brittany L. , Costello Brian A. , Morris Jonathan M. , Lucido John J. , Olivier Kenneth R. , Stish Brad , Laack Nadia N. , Park Sean , Owen Dawn , Ghia Amol J. , Brown Paul D. , Merrell Kenneth Wing
Published: March 2023

Background

Though metastasis-directed therapy (MDT) has the potential to improve overall survival (OS), appropriate patient selection remains challenging. We aimed to develop a model predictive of OS to refine patient selection for clinical trials and MDT.

Patients and methods

We assembled a multi-institutional cohort of patients treated with MDT (stereotactic body radiation therapy, radiosurgery, and whole brain radiation therapy). Candidate variables for recursive partitioning analysis were selected per prior studies: ECOG performance status, time from primary diagnosis, number of additional non-target organ systems involved (NOS), and intracranial metastases.

Results

A database of 1,362 patients was assembled with 424 intracranial, 352 lung, and 607 spinal treatments (n=1,383). Treatments were split into training (TC) (70%, n=968) and internal validation (IVC) (30%, n=415) cohorts. The TC had median ECOG of 0 (interquartile range [IQR]: 0-1), NOS of 1 (IQR: 0-1), and OS of 18 months (IQR: 7-35). The resulting model components and weights were: ECOG = 0, 1, and > 1 (0, 1, and 2); 0, 1, and > 1 NOS (0, 1, and 2); and intracranial target (2), with lower scores indicating more favorable OS. The model demonstrated high concordance in the TC (0.72) and IVC (0.72). The score also demonstrated high concordance for each target site (spine, brain, and lung).

Conclusion

This pre-treatment decision tool represents a unifying model for both intracranial and extracranial disease and identifies patients with the longest survival after MDT who may benefit most from aggressive local therapy. Carefully selected patients may benefit from MDT even in the presence of intracranial disease, and this model may help guide patient selection for MDT.
Preclinical modeling of lower-grade gliomas
Authors: Tang Lilly W. , Mallela Arka N. , Deng Hansen , Richardson Timothy E. , Hervey-Jumper Shawn L. , McBrayer Samuel K. , Abdullah Kalil G.
Published: March 2023

Models for human gliomas prove critical not only to advancing our understanding of glioma biology but also to facilitate the development of therapeutic modalities. Specifically, creating lower-grade glioma (LGG) models has been challenging, contributing to few investigations and the minimal progress in standard treatment over the past decade. In order to reliably predict and validate the efficacies of novel treatments, however, LGG models need to adhere to specific standards that recapitulate tumor genetic aberrations and micro-environment. This underscores the need to revisit existing models of LGG and explore prospective models that may bridge the gap between preclinical insights and clinical translation. This review first outlines a set of criteria aimed to address the current challenges hindering model development. We then evaluate the strengths and weaknesses of existing preclinical models of LGG with respect to these established standards. To conclude, the review discusses potential future directions for integrating existing models to maximize the exploration of disease mechanisms and therapeutics development.
Real-life monocentric experience of venetoclax-based regimens for acute myeloid leukemia
Authors: Sciumè Mariarita , Bosi Alessandro , Canzi Marta , Ceparano Giusy , Serpenti Fabio , De Roberto Pasquale , Fabris Sonia , Tagliaferri Elena , Cavallaro Francesca , Onida Francesco , Fracchiolla Nicola Stefano
Published: March 2023

Introduction

Combination of venetoclax and hypomethylating agents (HMAs) has become a standard of care in acute myeloid leukemia (AML) aged >75 years or who have comorbidities that preclude intensive induction chemotherapy.

Methods

We conducted a monocentric retrospective analysis on adult patients affected by treatment-naïve AML not eligible for standard induction therapy or refractory/relapsed (R/R) AML treated with venetoclax combinations outside clinical trials. Venetoclax was administered at the dose of 400 mg/daily after a short ramp-up and reduced in case of concomitant CYP3A4 inhibitors.

Results

Sixty consecutive AML were identified. Twenty-three patients (38%) were affected by treatment-naïve AML and 37 (62%) by R/R AML. Median age was 70 years. Among R/R AML 30% had received a prior allogeneic stem cell transplantation (allo-HSCT). In combination with venetoclax, 50 patients (83%) received azacitidine. Antifungal prophylaxis was performed in 33 patients (55%).

Overall response rate was 60%, with 53% of complete remission (CR; 78% for treatment-naïve and 49% for R/R, p 0.017). Median overall survival was 130 days for R/R patients and 269 days for treatment-naïve patients; median event free survival was 145 days for R/R cohort and 199 days for treatment-naïve AML.

Measurable residual disease was negative in 26% of evaluable patients in CR/CR with incomplete hematologic recovery after 2 cycles and in 50% after 4 cycles, with no significant association with survival.

Eleven patients (18%) received an allo-HSCT after venetoclax combinations. Most common grade 3/4 adverse events were infectious (51% of the patients), or hematological without infections (25% of the patients). Use of CYP3A4 inhibitors was associated with a trend to shorter cytopenias and with a lower rate of infections. Invasive fungal infections were less frequent among patients receiving azole prophylaxis (6% vs 26%; p 0.0659).

Discussion

Venetoclax-based regimens are a viable option for AML considered not eligible for standard induction therapy and a valid rescue therapy in the R/R setting.

Azole prophylaxis did not significantly affect response and it was associated with a lower rate of invasive fungal infections. Despite a limited number of patients, the association of venetoclax and HMAs proved to be also a feasible bridging therapy to transplantation.
Impact of in utero drug exposure on neonates requiring ECMO: A retrospective cohort study
Authors: Walther Hallie , Schadler Aric , Garlitz Karen , Bauer John A. , Kohler Lindsay , Waldsmith Erika , Ballard Hubert O.
Published: March 2023

The incidence of in utero drug exposure (IUDE) and neonatal extracorporeal membrane oxygenation (ECMO) utilization have both increased over the past decade. However, there are no studies to date that examine the impact that IUDE has on neonates requiring ECMO. In this retrospective cohort study, we compared the clinic course and outcomes of neonates who were placed on ECMO with IUDE vs. neonates without IUDE. Analysis included data extracted from medical records from all neonatal ECMO runs between January 2014 and January 2021 at the University of Kentucky Children's Hospital. A total of 56 neonatal patients were placed on ECMO during this time period and there were a total of 57 ECMO runs. Nearly one-third of neonates (16) had documented IUDE. There were no differences in gestational age, length of ECMO run, survival to discharge, or number of major complications while on ECMO in the neonates with IUDE compared to those without. In contrast, greater use of sedative and analgesic adjuvant medications during ECMO was required for IUDE-ECMO cases (p < 0.01). Trending results indicated that post-ECMO feeding complications and total hospitalization length were also greater in the IUDE-ECMO group. These findings illustrate the complex influence of prenatal drug exposures on neonatal patient care and warrant the development of clinical care strategies optimized for this unique patient group.
Serum neutralizing capacity and T-cell response against the omicron BA.1 variant in seropositive children and their parents one year after SARS-CoV-2 infection
Authors: Seidel Alina , Jacobsen Eva-Maria , Fabricius Dorit , Class Magdalena , Zernickel Maria , Blum Carmen , Conzelmann Carina , Weil Tatjana , Groß Rüdiger , Bode Sebastian F. N. , Renk Hanna , Elling Roland , Stich Maximillian , Kirchhoff Frank , Debatin Klaus-Michael , Münch Jan , Janda Aleš
Published: March 2023

Introduction

Durability of immune protection against reinfection with SARS-CoV-2 remains enigmatic, especially in the pediatric population and in the context of immune-evading variants of concern. Obviously, this knowledge is required for measures to contain the spread of infection and in selecting rational preventive measures.

Methods

Here, we investigated the serum neutralization capacity of 36 seropositive adults and 34 children approximately one year after infection with the ancestral Wuhan strain of SARS-CoV-2 by using a pseudovirus neutralization assay.

Results

We found that 88.9% of seropositive adult (32/36) and 94.1% of seropositive children (32/34) convalescents retained the neutralizing activity against the SARS-CoV-2 Wuhan strain (WT). Although, the neutralization effect against Omicron BA.1 (B.1.1.529.1) was significantly lower, 70.6% (24/34) of children and 41.7% (15/36) of adults possessed BA.1 cross-neutralizing antibodies. The spike 1 (S1)-specific T cell recall capacity using an activation-induced marker assay was analyzed in 18 adults and 16 children. All participants had detectable S1-specific CD4 T cells against WT, and 72.2% (13/18) adults and 81,3% (13/16) children had detectable S1 WT-specific CD8 T cells. CD4 cross-reactivity against BA.1 was demonstrated in all investigated adults (18/18), and 66.7% (12/18) adult participants had also detectable specific CD8 BA.1 T cells while we detected BA.1 S1 reactive CD4 and CD8 T cells in 81.3% (13/16) children.

Discussion

Together, our findings demonstrate that infection with the ancestral strain of SARS-CoV-2 in children as well as in adults induces robust serological as well as T cell memory responses that persist over at least 12 months. This suggests persistent immunological memory and partial cross-reactivity against Omicron BA.1.
Treatment with paeoniflorin increases lifespan of Pseudomonas aeruginosa infected Caenorhabditis elegans by inhibiting bacterial accumulation in intestinal lumen and biofilm formation
Authors: Wang Yuxing , Zhang Le , Yuan Xiaoan , Wang Dayong
Published: March 2023

Paeoniflorin is one of the important components in Paeoniaceae plants. In this study, we used Caenorhabditis elegans as a model host and Pseudomonas aeruginosa as a bacterial pathogen to investigate the possible role of paeoniflorin treatment against P. aeruginosa infection in the host and the underlying mechanisms. Posttreatment with 1.25–10 mg/L paeoniflorin could significantly increase the lifespan of P. aeruginosa infected nematodes. After the infection, the P. aeruginosa colony-forming unit (CFU) and P. aeruginosa accumulation in intestinal lumen were also obviously reduced by 1.25–10 mg/L paeoniflorin treatment. The beneficial effects of paeoniflorin treatment in increasing lifespan in P. aeruginosa infected nematodes and in reducing P. aeruginosa accumulation in intestinal lumen could be inhibited by RNAi of pmk-1, egl-1, and bar-1. In addition, paeoniflorin treatment suppressed the inhibition in expressions of pmk-1, egl-1, and bar-1 caused by P. aeruginosa infection in nematodes, suggesting that paeoniflorin could increase lifespan of P. aeruginosa infected nematode by activating PMK-1, EGL-1, and BAR-1. Moreover, although treatment with 1.25–10 mg/L paeoniflorin did not show obvious anti-P. aeruginosa activity, the P. aeruginosa biofilm formation and expressions of related virulence genes (pelA, pelB, phzA, lasB, lasR, rhlA, and rhlC) were significantly inhibited by paeoniflorin treatment. Treatment with 1.25–10 mg/L paeoniflorin could further decrease the levels of related virulence factors of pyocyanin, elastase, and rhamnolipid. In addition, 2.5–10 mg/L paeoniflorin treatment could inhibit the swimming, swarming, and twitching motility of P. aeruginosa, and treatment with 2.5–10 mg/L paeoniflorin reduced the cyclic-di-GMP (c-di-GMP) level. Therefore, paeoniflorin treatment has the potential to extend lifespan of P. aeruginosa infected hosts by reducing bacterial accumulation in intestinal lumen and inhibiting bacterial biofilm formation.
Alternative schemes for twin-field quantum key distribution with discrete-phase-randomized sources
Authors: Li Huaicong , Zhang Chunmei
Published: March 2023

The twin-field quantum key distribution (TF-QKD) protocol and its variants can overcome the well-known rate-loss bound without quantum repeaters, which have attracted significant attention. Generally, to ensure the security of these protocols, weak coherent states with continuous randomized phases are always assumed in the test mode. However, this assumption is difficult to meet in practice. To bridge the gap between theory and practice, we propose two alternative discrete-phase-randomized (DPR)-twin-field quantum key distribution protocols, which remove the phase sifting procedure in the code mode. Simulation results show that when compared with previous discrete-phase-randomized-twin-field quantum key distribution protocols, our modified protocols can significantly improve the secret key rate in the low channel loss range, which is very promising for practical twin-field quantum key distribution systems.
Effects of dietary substitution of fishmeal by black soldier fly (Hermetia illucens) meal on growth performance, whole-body chemical composition, and fatty acid profile of Pontastacus leptodactylus juveniles
Authors: Alvanou Maria V. , Kyriakoudi Anastasia , Makri Vasiliki , Lattos Athanasios , Feidantsis Konstantinos , Papadopoulos Dimitrios K. , Georgoulis Ioannis , Apostolidis Apostolos P. , Michaelidis Basile , Mourtzinos Ioannis , Asimaki Adamantia , Karapanagiotidis Ioannis T. , Giantsis Ioannis A.
Published: March 2023

Freshwater crayfish are considered as aquatic products of high quality and high nutritional value. The increasing demand has led to populations reduction in several locations throughout their range. Thus, the development of appropriate rearing conditions is considered necessary, among which, optimization of their diet is a basic part. Towards this direction, in the present study, a 98-day feeding trial was carried out to evaluate the impact of dietary fishmeal substitution by Hermetia illucens meal on Pontastacus leptodactylus juveniles kept under laboratory conditions. Insect meals represent an environmentally friendly alternative solution, considered as a high-value feed source, rich in nutrients such as protein and fat. Three dietary regimens were utilized with a fishmeal-based without Hermetia meal (HM) defined as the control diet (HM0), and two diets, the first with 50% (HM50) and the second with 100% (HM100) of fishmeal substitution by HM, respectively. Growth performance, whole-body composition, and fatty acid profiles of individuals were studied in the different treatments. At the end of the feeding trial, statistically significant differences were observed in the mean survival rate (SR), specific growth rate (SGR), feed conversion ratio (FCR) and weight gain (WG) values. More specifically, animals fed with HM-based diets had higher mean SR, while the control group performed better regarding FCR and SGR. The HM inclusion in the diet significantly altered the whole-body chemical composition of the crayfish signifying a different metabolic utilization compared to fishmeal (FM). The fatty acid analysis revealed that 16:0 (palmitic acid) was the predominant saturated fatty acid (SFA), 18:1ω9 (oleic acid) was found to be the main monounsaturated fatty acid (MUFA), while 18:2ω6 (linoleic acid) represented the major polyunsaturated fatty acid (PUFA) followed by C20:3 cis ω3 (cis-11-14-17-eicosatrienoate) and C22:6 cis ω3 (cis-4,7,10,13,16,19-Docosahexaenoic) fatty acids. The inclusion of dietary HM significantly reduced the contents of ∑SFAs, ∑PUFAs and ∑ω6 fatty acids, as well as those of C22:6 cis ω3 and increased the ω6/ω3 and hypocholesterolemic to hypercholesterolemic ratios in the body. In parallel with improvements in balanced diets and in culture conditions that need to be optimised for rearing of freshwater crayfish, our study provides new data that enlighten the suitability of insect meals in the nutrition of P. leptodactylus.
Identification of simple sequence repeat markers linked to heat tolerance in rice using bulked segregant analysis in F2 population of NERICA-L 44 × Uma
Authors: Stephen K. , Aparna K. , Beena R. , Sah R. P. , Jha Uday Chand , Behera Sasmita
Published: March 2023

The damage caused by high temperature is one of the most important abiotic stress affecting rice production. Reproductive stage of rice is highly susceptible to high temperature. The present investigation was undertaken to identify polymorphic microsatellite markers (SSR) associated with heat tolerance. The rice cultivars NERICA– L 44 (heat tolerant) and Uma (heat susceptible) were crossed to generate F1 and F2 populations. The F2 population was subjected to heat stress at >38°C and the 144 F2 plants were evaluated for their tolerance. The results note that the mean of the F2 population was influenced by the tolerant parent with regards to the traits of plant height, membrane stability index, photosynthetic rate, stomatal conductance, evapotranspiration rate, pollen viability, spikelet fertility and 1000 grain weight. Ten each of the extremely susceptible and tolerant plants were selected based on the spikelet fertility percentage. Their DNA was pooled into tolerant and susceptible bulks and Bulked Segregant Analysis (BSA) was carried out using 100 SSR markers to check for polymorphism. The survey revealed a polymorphism of 18% between the parents. RM337, RM10793, RM242, RM5749, RM6100, RM490, RM470, RM473, RM222 and RM556 are some of the prominent markers that were found to be polymorphic between the parents and the bulks. We performed gene annotation and enrichment analysis of identified polymorphic markers. Result revealed that the sequence specific site of that chromosome mostly enriched with biological processes like metabolic pathway, molecular mechanism, and subcellular function. Among that RM337 was newly reported marker for heat tolerance. Expression analysis of two genes corresponds to RM337 revealed that LOP1 (LOC_Os08g01330) was linked to high temperature tolerance in rice. The results demonstrate that BSA using SSR markers is useful in identifying genomic regions that contribute to thermotolerance.
Genetic and molecular analysis of the anthocyanin pigmentation pathway in Epimedium
Authors: Mi Yaolei , He Ruikun , Wan Huihua , Meng Xiangxiao , Liu Di , Huang Wenjun , Zhang Yanjun , Yousaf Zubaida , Huang Hongwen , Chen Shilin , Wang Ying , Sun Wei
Published: March 2023

Introduction

Flower color is an ideal trait for studying the molecular basis for phenotypic variations in natural populations of species. Epimedium (Berberidaceae) species exhibit a wide range of flower colors resulting from the varied accumulation of anthocyanins and other pigments in their spur-like petals and petaloid sepals.

Methods

In this work, the anthocyanidins of eight different Epimedium species with different floral pigmentation phenotypes were analyzed using HPLC. Twelve genes involved in anthocyanin biosynthesis were cloned and sequenced, and their expression was quantified.

Results

The expression levels of the catalytic enzyme genes DFR and ANS were significantly decreased in four species showing loss of floral pigmentation. Complementation of EsF3’H and EsDFR in corresponding Arabidopsis mutants together with overexpression of EsF3’5’H in wild type Arabidopsis analysis revealed that these genes were functional at the protein level, based on the accumulation of anthocyanin pigments.

Discussion

These results strongly suggest that transcriptional regulatory changes determine the loss of anthocyanins to be convergent in the floral tissue of Epimedium species.
Non-destructive prediction and visualization of anthocyanin content in mulberry fruits using hyperspectral imaging
Authors: Li Xunlan , Wei Zhaoxin , Peng Fangfang , Liu Jianfei , Han Guohui
Published: March 2023

Being rich in anthocyanin is one of the most important physiological traits of mulberry fruits. Efficient and non-destructive detection of anthocyanin content and distribution in fruits is important for the breeding, cultivation, harvesting and selling of them. This study aims at building a fast, non-destructive, and high-precision method for detecting and visualizing anthocyanin content of mulberry fruit by using hyperspectral imaging. Visible near-infrared hyperspectral images of the fruits of two varieties at three maturity stages are collected. Successive projections algorithm (SPA), competitive adaptive reweighted sampling (CARS) and stacked auto-encoder (SAE) are used to reduce the dimension of high-dimensional hyperspectral data. The least squares-support vector machine and extreme learning machine (ELM) are used to build models for predicting the anthocyanin content of mulberry fruit. And genetic algorithm (GA) is used to optimize the major parameters of models. The results show that the higher the anthocyanin content is, the lower the spectral reflectance is. 15, 7 and 13 characteristic variables are extracted by applying CARS, SPA and SAE respectively. The model based on SAE-GA-ELM achieved the best performance with R2 of 0.97 and the RMSE of 0.22 mg/g in both the training set and testing set, and it is applied to retrieve the distribution of anthocyanin content in mulberry fruits. By applying SAE-GA-ELM model to each pixel of the mulberry fruit images, distribution maps are created to visualize the changes in anthocyanin content of mulberry fruits at three maturity stages. The overall results indicate that hyperspectral imaging, in combination with SAE-GA-ELM, can help achieve rapid, non-destructive and high-precision detection and visualization of anthocyanin content in mulberry fruits.
Integrated analysis of transcriptomics and metabolomics of peach under cold stress
Authors: Li Yonghong , Tian Qihang , Wang Zhaoyuan , Li Jie , Liu Shiyuan , Chang Ruifeng , Chen Hu , Liu Guojian
Published: March 2023

Low temperature is one of the environmental factors that restrict the growth and geographical distribution of peach (Prunus persica L. Batsch). To explore the molecular mechanisms of peach brunches in response to cold, we analyzed the metabolomics and transcriptomics of ‘Donghe No.1’ (cold-tolerant, CT) and ‘21st Century’ (cold-sensitive, CS) treated by different temperatures (-5 to -30°C) for 12 h. Some cold-responsive metabolites (e.g., saccharides, phenolic acids and flavones) were identified with upregulation only in CT. Further, we identified 1991 cold tolerance associated genes in these samples and they were significantly enriched in the pathways of ‘galactose metabolism’, ‘phenylpropanoid biosynthesis’ and ‘flavonoids biosynthesis’. Weighted gene correlation network analysis showed that soluble sugar, flavone, and lignin biosynthetic associated genes might play a key role in the cold tolerance of peach. In addition, several key genes (e.g., COMT, CCR, CAD, PER and F3’H) were substantially expressed more in CT than CS under cold stress, indicating that they might be major factors during the adaptation of peach to low temperature. This study will not only improve our understanding towards the molecular mechanisms of peach trees under cold stress but also contribute to the screening and breeding program of peach in the future.
African coups in the COVID-19 era: A current history
Authors: Chin John J. , Kirkpatrick Jessica
Published: March 2023

What explains the explosion of coup activity in Africa over the last few years? To answer this question, this article presents narrative summaries—a current history—of all eleven coups attempts in Africa between August 2020 and November 2022. We then discuss the most relevant causal explanations for the observed increase in coup frequency in Africa in this period. Though we find relatively little evidence of direct coup diffusion or democratic backsliding as coup triggers, our findings suggest that coup-struck African countries over the last few years are disproportionately poor, have a recent history of coups, and face ongoing dilemmas of democratic consolidation. Ongoing Islamist insurgencies may have helped precipitate recent coups in West Africa but not elsewhere.
The moderating role of spirituality and gender in Canadian and Iranian emerging adolescents’ theory of mind and prosocial behavior
Authors: Khalili Nadia , Bosacki Sandra , Talwar Victoria
Published: March 2023

Introduction

While research has found a link between ToM and prosociality in terms of caring and helping others which may also vary across cultures, the moderating role of spirituality and culture of this association in emerging adolescence has received little attention.

Methods

The current study empirically “examined” the role of spirituality and gender in relation to ToM and prosocial behavior in Canadian and Iranian emerging adolescents. A total of 300 (153 girls) emerging adolescents (M = 11.502, SD = 2.228) were recruited from Montreal, Canada and Karaj, Iran. A series of double moderation analysis and ANOVA was conducted.

Results and discussion

Results indicated the difference between direct and indirect influences of ToM and its interactions with culture, gender, and spirituality on prosocial behavior. This implies an emerging complex framework which suggests the dynamic nonlinear interactions between these factors. Implications for youth’s social-emotional understanding will be discussed.
The relationship between urinary selenium levels and risk of gestational diabetes mellitus: A nested case–control study
Authors: Liu Yuanxia , Chen Hongmei , Zhang Mengtian , Zhu Gangjiao , Yang Yan , Li Yuanyuan , Lu Wei , Zhang Hongling
Published: March 2023

Background

Selenium (Se) is an essential trace element for the human body. Serum Se and urinary Se are also biomarkers to assess Se exposure status. However, studies focusing on the association between urinary Se and the risk of gestational diabetes mellitus (GDM) are rare.

Objective

To investigate the association between urinary Se and the risk of GDM.

Methods

A nested case–control study based on a prospective birth cohort in Wuhan, China, which focuses on the effects of prenatal environmental factors exposure on pregnant women and children’s health was conducted. Two hundred and twenty-six cases and 452 controls were included. Maternal urine samples were collected before GDM diagnosis, and the urinary Se levels were determined. We assessed the association of urinary Se with GDM by conditional logistic regression with maternal urinary Se level as a categorical variable, and estimated the association between Se and glucose levels by multiple linear regression. The potential modifier roles of maternal age and fetal sex have also been assessed.

Results

Lower urinary level of Se was significantly associated with a higher risk of GDM (OR = 2.35 for the tertile 1, 95% CI:1.36-4.06; adjusted OR = 1.79 for the tertile 2, 95%CI:1.09-2.95; p for trend = 0.01). Fetal sex had an interaction with Se in the association with GDM. The association was more pronounced among pregnant women with female fetuses than with male fetuses.

Discussion

Our study suggested a significant negative association between urinary Se and the risk of GDM, and this association may vary depending on the fetal sex.
Radiological outcomes of PEEK rods in patients with lumbar degenerative diseases: A minimum 5-year follow-up
Authors: Huang Weimin , Wang Wenqiao , Xu Xiaoduo , Wang Lei , Wang Jingming , Yu Xiuchun
Published: March 2023

Purpose

To determine the long-term radiological outcomes of PEEK rods in patients with lumbar degenerative diseases.

Methods

Radiological outcomes of cohort cases with lumbar degenerative diseases following PEEK rods were retrospectively studied. Disc height index (DHI) and range of motion (ROM) were measured by x-rays. The CT scans and reconstruction were used to determine screw breakage, rods fracture, screw loosening and intervertebral bony fusion status. The MRI scans were used to evaluate the changes of intervertebral discs at the non-fusion segments and adjacent segments in terms of Pfirrmann Classification.

Results

A total of 40 patients completed the mean of 74.8 ± 9.6 months follow-up, with 32 patients undergoing hybrid surgery and 8 patients undergoing non-fusion surgery. The mean DHI changed from preoperative 0.34 to 0.36 at the final follow-up and the ROM declined from 8.8° preoperatively to 3.2° at the final visit, however, both had no statistical differences. Of the 40 levels underwent non-fusion procedure, 9 levels showed disc rehydration with 7 patients from Grade 4 to Grade 3 and 2 patients from Grade 3 to Grade 2. The other 30 cases did not show distinctive change. No screw loosening or rods breakage were detected during the follow-up periods.

Conclusion

PEEK rods have obvious protective effects on degenerated intervertebral disc of non-fusion segments and the incidence of complications related to internal fixation is low. PEEK rods pedicle screw system is safe and effective in the treatment of lumbar degenerative diseases.
A Decision-Making Model for Selecting Product Suppliers in Crop Protection Retail Sector
Authors: Ahn Byungok , Kim Boyoung
Published: March 2023

This study aims to determine the importance of factors affecting supplier selection in the pesticide distribution sector as a global emerging market and present a decision-making model for the corporate marketing strategy. Specifically, a comparative study between suppliers and retail distribution experts was conducted to compare differences in the perception of supplier selection factors according to organizational characteristics. Based on previous studies, a decision-making model based on the AHP methodology was constructed with a total of 20 factors in five areas: product quality, price, flexibility, promotion support, and brand. Then, 42 Korean experts were surveyed to measure the importance of these factors. The results showed that product quality is the most critical factor in supplier selection, followed by price, brand, promotional support, and flexibility, in that order. Manufacturers consider product quality as the most important factor, while retailers consider price as the most important factor. Among the 20 factors, ‘quality excellence’, ‘expected return’, and ‘technological competitiveness’ were found to be the most important factors. In addition, while manufacturers considered factors such as ‘corporate reputation’ and ‘corporate trust’ as more important, retailers considered factors related to product characteristics, such as ‘product awareness’ and ‘brand reputation’ as more important.
Design and Testing of Impacted Stiffened CFRP Panels under Compression with the VERTEX Test Rig
Authors: Grotto Florent , Bouvet Christophe , Castanié Bruno , Serra Joël
Published: March 2023

Aeronautical composite primary structures must evidence sufficient residual strength in the presence of damage for compliance with damage tolerance requirements. The study of stiffener debonding on panels subjected to compression after impact is performed in that scope. Compression leads to the buckling of the skin between the stiffeners, and thus a complex loading of the bonding between the skin and the stiffener. This paper describes the development of a stiffened specimen for the VERTEX multiaxial test rig as a first step towards the study of the damage tolerance evaluation of stiffened structures, under combined loadings and at the intermediate scale of the test pyramid. By using virtual testing, the specimen was designed to produce the phenomenology of interest as the first damage, i.e., the debonding of the stiffener from the centre. Three samples were manufactured and subjected to low velocity impacts at various locations and energies. Then the three samples were subjected to compression after impact, up to the stiffener debonding, under a post-buckling regime of the skin. Test loading evolution is described with force fluxes and global strains, obtained from in situ stereo-correlation. The different impacts were found to give different types of damage but similar residual strength to compression after impact.