bluuebunny/crossref_metadata_2025 huggingface dataset:
The growth and production of some fast growing deciduous tree species stands on abandoned agricultural land
Authors: Uri Veiko , Aosaar Jürgen , Varik Mats , Kund Merit
Published: March 2011

Mõningate kiirekasvuliste lehtpuupuistute kasv ja produktsioonivõime endisel põllumaal

Several studies about stands growing on abandoned agricultural lands are induced by extensive afforestation of agricultural lands and more intensive use of biomass. Overview of above-ground biomass production of grey alder, hybrid alder and silver birch young stands growing on former agricultural areas are presented in current paper. The results of 16- and 6-year period of alders and silver birch stands, respectively, are reported. Above-ground biomass and biomass production of stands were estimated. The growing stock and current annual increment (CAI) of 16-years-old grey alder stand were 250 m3 ha-1 and 35.6 m3 ha-1, respectively. Due to fast growth and high biomass production capacity grey alder is promising tree species for short-rotation forestry in Estonia. Hybrid alder is productive tree although not exceeding the productivity of grey alders. Stem volume and CAI of the 16-years-old hybrid alder stand were 155 m3 ha-1 and 21 m3 ha-1, respectively. Our results supported earlier data reported in literature: rotation period of hybrid alder is longer than for grey alder and CAI of hybrid alder stands culminate later. The productivity of young silver birch stands on abandoned agricultural land is varying in a broad range. One young silver birch stand growing on abandoned agricultural land was involved into study. Growing stock and CAI in 13-years old stand were 118 m3 ha-1 and 15 m3 ha-1, respectively. These values are exceeding respective values of several yield tables of silver birch and biomass production capability of silver birch stands in favourable conditions is high.
Use of biofuel ashes in forestry
Authors: Pärn Henn , Mandre Malle , Ots Katri , Klõšeiko Jaan , Lukjanova Aljona , Kuznetsova Tatjana
Published: March 2011

Bioenergeetikas tekkivate jäätmete kasutamine metsanduses

Negative environmental impacts of fossil fuel combustion have increased the use of biomass for energy production. As a consequence of the increased use of biofuels, the production of ashes will increase greatly in the near future. Because of relatively high contents of plant nutrients, biofuel ashes can be recycled as mineral fertilisers to compensate for the loss of nutrients resulting from tree harvesting. The present review aims to summarise the available information on factors affecting the quality of the wood and peat ash and the implications arising from ash application as fertiliser in the forest ecosystems. The understanding of the wood ash as fertiliser originates from the traditional slash-and-burn agriculture. During combustion most elements in wood are retained in the ash. The quality and chemical composition of ashes depends on many factors. The major elements in the ashes are calcium, potassium, magnesium, manganese, sodium, iron, phosphorus and sulphur. Trace elements found in different ash include barium, boron, cadmium, copper, mercury and zinc. The most abundant elements in peat ash are silicon, iron and aluminium. Ashes are characterised by high alkalinity with pH in the range from 11 to 13. Ashes raise the pH and reduce the total acidity in the humus layer and in the top of the mineral soil. The addition of wood ash does not result in a significant growth increase on mineral soils but increases the tree growth on peat soils. The Cd in wood ash did not become bioavailable and harmful to forest biota. The application of wood ash did not change or even decreased the 137Cs activity within forest soil. Wood ash application increased the coverage of the ground vegetation in upland forests and on peatland. Increase in microbial activity and growth rate after ash treatment was observed.
Stimulating the growth of trees with ashes of various biofuels (wood, peat) on a cutaway peatland
Authors: Kikamägi Karin , Ots Katri
Published: March 2011

Puittaimede kasvu stimuleerimine erinevate biokütuste (puit, turvas) tuha liikidega ammendatud freesturbaväljal

The aim of the study was to investigate the effect of fertilization on the growth of Silver birch Betula pendula Roth and Scots pine Pinus sylvestris L. seedlings on a cutaway peatland (Ulila, 58°22'N, 26°26'E). Five treatments were established: wood ash (10 000 and 5000 kg/ha), peat and wood ash mixture (10 000 and 5000 kg/ha) and control (unfertilized field). Results of analysis showed that the pH of peat in the cutaway peatland was before treatment 3.5 and it rose after fertilization by up to 0.7 units by the end of the first growing season. The treatment with 10 000 kg/ha of wood ash gave the best results: the annual height increment of Silver birches during the first growing season after fertilization was 4.5 times higher and that of Scots pines 1.1 times higher than control. In the second growing season after fertilization the increment was respectively 16.3 and 3.3 times higher than control. The root collar diameters of Silver birches were 4.4 time larger these of Scots pines 2.1 times larger than control. Scots pine needles were 2.2 times longer than control and the leaf area of the Silver birches was 6 times larger than control, which also shows a positive effect of fertilization. Peat ash treatment was also favourable, although its effect was much smaller than the effect of wood ash.
Changes in Scholarly Influence in Major International Criminology Journals, 1986–2005
Authors: Cohn Ellen G.
Published: March 2011

Changes in scholarly influence in four major international criminology journals (CJC—Canadian Journal of Criminology and Criminal Justice; CRIM—Criminology; BJC—British Journal of Criminology; ANZ—Australian and New Zealand Journal of Criminology) were measured by determining the most-cited scholars in 2001–2005 and comparing them with the most-cited scholars in 1996–2000, 1991–1995, and 1986–1990. The number of cited authors increased by over 90% between 1986–1990 and 2001–2005. The most-cited scholars in 2001–2005 were Julian V. Roberts in CJC, Robert J. Sampson in CRIM, John Braithwaite in BJC, and Lawrence W. Sherman in ANZ. There was clear concordance between CJC and CRIM, and between BJC and ANZ, in the most-cited authors. The analyses reveal the increasing scholarly influence of some authors over this 20-year time period, the decreasing scholarly influence of others, and the continuing high influence of others. A list of the most-cited works of the most-cited authors showed that some scholars were specialized, with a large number of citations of one or two seminal works, usually books and often theoretical in nature. Other scholars were versatile: they had many different works cited a few times each.
Quantitative fluorescence in intracranial tumor: implications for ALA-induced PpIX as an intraoperative biomarker
Authors: Valdés Pablo A. , Leblond Frederic , Kim Anthony , Harris Brent T. , Wilson Brian C. , Fan Xiaoyao , Tosteson Tor D. , Hartov Alex , Ji Songbai , Erkmen Kadir , Simmons Nathan E. , Paulsen Keith D. , Roberts David W.
Published: March 2011

Object

Accurate discrimination between tumor and normal tissue is crucial for optimal tumor resection. Qualitative fluorescence of protoporphyrin IX (PpIX), synthesized endogenously following δ-aminolevulinic acid (ALA) administration, has been used for this purpose in high-grade glioma (HGG). The authors show that diagnostically significant but visually imperceptible concentrations of PpIX can be quantitatively measured in vivo and used to discriminate normal from neoplastic brain tissue across a range of tumor histologies.

Methods

The authors studied 14 patients with diagnoses of low-grade glioma (LGG), HGG, meningioma, and metastasis under an institutional review board–approved protocol for fluorescence-guided resection. The primary aim of the study was to compare the diagnostic capabilities of a highly sensitive, spectrally resolved quantitative fluorescence approach to conventional fluorescence imaging for detection of neoplastic tissue in vivo.

Results

A significant difference in the quantitative measurements of PpIX concentration occurred in all tumor groups compared with normal brain tissue. Receiver operating characteristic (ROC) curve analysis of PpIX concentration as a diagnostic variable for detection of neoplastic tissue yielded a classification efficiency of 87% (AUC = 0.95, specificity = 92%, sensitivity = 84%) compared with 66% (AUC = 0.73, specificity = 100%, sensitivity = 47%) for conventional fluorescence imaging (p < 0.0001). More than 81% (57 of 70) of the quantitative fluorescence measurements that were below the threshold of the surgeon's visual perception were classified correctly in an analysis of all tumors.

Conclusions

These findings are clinically profound because they demonstrate that ALA-induced PpIX is a targeting biomarker for a variety of intracranial tumors beyond HGGs. This study is the first to measure quantitative ALA-induced PpIX concentrations in vivo, and the results have broad implications for guidance during resection of intracranial tumors.
Outcomes of 33 patients from the wars in Iraq and Afghanistan undergoing bilateral or bicompartmental craniectomy
Authors: Ecker Robert D. , Mulligan Lisa P. , Dirks Michael , Bell Randy S. , Severson Meryl A. , Howard Robin S. , Armonda Rocco A.
Published: March 2011

Object

There are no published long-term data for patients with penetrating head injury treated with bilateral supratentorial craniectomy, or supra- and infratentorial craniectomy. The authors report their experience with 33 patients treated with bilateral or bicompartmental craniectomy from the ongoing conflicts in Iraq and Afghanistan.

Methods

An exploratory analysis of Glasgow Outcome Scale (GOS) scores at 6 months in 33 patients was performed. Follow-up lasting a median of more than 2 years was performed in 30 (91%) of these patients. The association of GOS score with categorical variables was explored using the Wilcoxon rank-sum test or Kruskal-Wallis analysis of variance. The Spearman correlation coefficient was used for ordinal/continuous data. To provide a clinically meaningful format to present GOS scores with categorical variables, patients with GOS scores of 1–3 were categorized as having a poor outcome and those with scores of 4 and 5 as having a good outcome. This analysis does not include the patients who died in theater or in Germany who underwent bilateral decompressive craniectomy because those figures have not been released due to security concerns.

Results

All patients were men with a median age of 24 years (range 19–46 years) and a median initial Glasgow Coma Scale (GCS) score of 5 (range 3–14). At 6 months, 9 characteristics were statistically significant: focus of the initial injury, systemic infection, initial GCS score, initial GCS score excluding patients with a GCS score of 3, GCS score on arrival to the US, GCS score on dismissal from the medical center, Injury Severity Score, and patients with cerebrovascular injury. Six factors were significant at long-term follow-up: focus of initial injury, systemic infection, initial GCS score excluding patients with a GCS score of 3, GCS score on arrival to the US, and GCS score on dismissal from the medical center. At long-term follow-up, 7 (23%) of 30 patients had died, 5 (17%) of 30 had a GOS score of 2 or 3, and 18 (60%) of 30 had a GOS score of 4 or 5.

Conclusions

In this selected group of patients who underwent bilateral or bicompartmental craniectomy, 60% are independent at long-term follow-up. Patients with bifrontal injury fared best. Systemic infection and cerebrovascular injury corresponded with a worse outcome.
Reconstructed bladder innervation above the level of spinal cord injury to produce urination by abdomen-to-bladder reflex contractions
Authors: Lin Haodong , Hou Chunlin , Chen Aimin
Published: March 2011

Neurogenic bladder dysfunction following spinal cord injury is a major medical and social problem for which there is no ideal treatment strategy. This paper describes the authors' attempts to establish bladder reinnervation, by establishing an abdomen-to-bladder reflex pathway, in a patient with an injury to the conus medullaris.
An Analytical Simple Formula for the Ground Level Concentration from a Point Source
Authors: Tirabassi Tiziano , Tiesi Alessandro , Vilhena Marco T. , Bodmann Bardo E.J. , Buske Daniela
Published: March 2011

The Advection-Diffusion Equation is solved for a constant pollutant emission from a point-like source placed inside an unstable Atmospheric Boundary Layer. The solution is obtained adopting the novel analytical approach: Generalized Integral Laplace Transform Technique. The concentration solution of the equation is expressed through an infinite series expansion. After setting a realistic scenario through the wind and diffusivity parameterizations, the Ground Level Concentration (GLC) is determined, and an explicit approximate expression is provided for it, allowing an analytically simple expression for the position and value of the maximum. Remarks arise regarding the ability to express value and position of the GLC as explicit functions of the parameters defining the Atmospheric Boundary Layer scenario and the source height.
Epidermal Growth Factor Receptor in Pancreatic Cancer
Authors: Oliveira-Cunha Melissa , Newman William G. , Siriwardena Ajith K.
Published: March 2011

Pancreatic cancer is the fourth leading cause of cancer related death. The difficulty in detecting pancreatic cancer at an early stage, aggressiveness and the lack of effective therapy all contribute to the high mortality. Epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein, which is expressed in normal human tissues. It is a member of the tyrosine kinase family of growth factors receptors and is encoded by proto-oncogenes. Several studies have demonstrated that EGFR is over-expressed in pancreatic cancer. Over-expression correlates with more advanced disease, poor survival and the presence of metastases. Therefore, inhibition of the EGFR signaling pathway is an attractive therapeutic target. Although several combinations of EGFR inhibitors with chemotherapy demonstrate inhibition of tumor-induced angiogenesis, tumor cell apoptosis and regression in xenograft models, these benefits remain to be confirmed. Multimodality treatment incorporating EGFR-inhibition is emerging as a novel strategy in the treatment of pancreatic cancer.
Application of the NOx Reaction Model for Development of Low-NOx Combustion Technology for Pulverized Coals by Using the Gas Phase Stoichiometric Ratio Index
Authors: Taniguchi Masayuki , Kamikawa Yuki , Shibata Tsuyoshi , Yamamoto Kenji , Kobayashi Hironobu
Published: March 2011

We previously proposed the gas phase stoichiometric ratio (SRgas) as an index to evaluate NOx concentration in fuel-rich flames. The SRgas index was defined as the amount of fuel required for stoichiometric combustion/amount of gasified fuel, where the amount of gasified fuel was the amount of fuel which had been released to the gas phase by pyrolysis, oxidation and gasification reactions. In the present study we found that SRgas was a good index to consider the gas phase reaction mechanism in fuel-rich pulverized coal flames. When SRgas < 1.0, NOx concentration was strongly influenced by the SRgas value. NOx concentration was also calculated by using a reaction model. The model was verified for various coals, particle diameters, reaction times, and initial oxygen concentrations. The most important reactions were gas phase NOx reduction reactions by hydrocarbons. The hydrocarbon concentration was estimated based on SRgas. We also investigated the ratio as an index to develop a new low-NOx combustion technology for pulverized coals. We examined the relation between local SRgas distribution in the fuel-rich region in the low-NOx flame and NOx emissions at the furnace exit, by varying burner structures. The relationship between local SRgas value and local NOx concentration was also examined. When a low-NOx type burner was used, the value of SRgas in the flame was readily decreased. When the local SRgas value was the same, it was difficult to influence the local NOx concentration by changing the burner structure. For staged combustion, the most important item was to design the burner structure and arrangement so that SRgas could be lowered as much as possible just before mixing with staged air.
Transport and Carbon Emissions in the United States: The Long View
Authors: Schipper Lee , Saenger Calanit , Sudardshan Anant
Published: March 2011

The following analysis traces U.S. transport CO2 emissions in combustion by mode for 1960–2008. Changes in emissions are divided into components related to overall population and economic growth, transport mode shift, changes in the ratio of fuel used to passenger or tonne-km of activity, and changes in the CO2 content of fuels. Where data permit we show how changes in vehicle utilization affected CO2 emissions. We comment on factors causing the changes in components of emissions. A Log-Mean Divisia Index and Laspeyres decompositions of the 1960–2008 changes are calculated. From this decomposition we speculate to what extent the factors associated with the increases in CO2 emissions since 1960 would be important in the future, and what other factors could reduce emissions. This thorough decomposition is imperative for the crafting of transport policy that aims to address climate change.
Clinical, Radiological, and Pathological Investigation of Asbestosis
Authors: Kishimoto Takumi , Kato Katsuya , Arakawa Hiroaki , Ashizawa Kazuto , Inai Kouki , Takeshima Yukio
Published: March 2011

By the radiological examination, differential diagnosis of asbestosis from chronic interstitial pneumonia such as IPF/UIP is difficult. The pathological features of asbestosis show the peribronchiolar fibrosis which suggest that asbestos fibers cause the inflammation of bronchioli. Therefore, the criteria for pathological diagnosis of asbestosis in 2010, contain the finding of peribronchiolar fibrosis again. Chest CT scanning including HRCT for total of 38 cases clinically diagnosed asbestosis were reviewed by 3 radiologists and one pulmonologist. On the other hand, the histology of lung tissues obtained by surgery or autopsy were examined by 4 pulmonological pathologists. Furthermore, the content of asbestos bodies in the lung was counted by phase-contrast microscopy. Thirteen cases were definitely diagnosed of asbestosis in the image including HRCT and 17 cases were diagnosed by the histopathological examination showing lung fibrosis with peribronchiolar fibrosis. Only 10 cases were indicated asbestosis by both the radiological and histopathological examinations. The mean value of asbestos bodies for these cases, was 2,133,255 per gram of dry lung tissue.
Continuous Spatial Tuning of Laser Emissions in a Full Visible Spectral Range
Authors: Jeong Mi-Yun , Wu Jeong Weon
Published: March 2011

In order to achieve a continuous tuning of laser emission, the authors designed and fabricated three types of cholesteric liquid crystal cells with pitch gradient, a wedge cell with positive slope, a wedge cell with negative slope, and a parallel cell. The length of the cholesteric liquid crystal pitch could be elongated up to 10 nm, allowing the lasing behavior of continuous or discontinuous spatial tuning determined by the boundary conditions of the cholesteric liquid crystal cell. In the wedge cell with positive slope, the authors demonstrated a continuous spatial laser tuning in the near full visible spectral range, with a tuning resolution less than 1 nm by pumping with only a single 355 nm laser beam. This continuous tuning behavior is due to the fact that the concentration of pitch gradient matches the fixed helical pitch determined by the cell thickness. This characteristic continuous spatial laser tuning could be confirmed again by pumping with a 532 nm laser beam, over 90 nm in the visible spectral range. The scheme of the spatial laser tuning in the wedge cell bearing a pitch gradient enabled a route to designing small-sized optical devices that allow for a wide tunability of single-mode laser emissions.
Amyloidogenic Properties of a D/N Mutated 12 Amino Acid Fragment of the C-Terminal Domain of the Cholesteryl-Ester Transfer Protein (CETP)
Authors: García-González Victor , Mas-Oliva Jaime
Published: March 2011

The cholesteryl-ester transfer protein (CETP) facilitates the transfer of cholesterol esters and triglycerides between lipoproteins in plasma where the critical site for its function is situated in the C-terminal domain. Our group has previously shown that this domain presents conformational changes in a non-lipid environment when the mutation D470N is introduced. Using a series of peptides derived from this C-terminal domain, the present study shows that these changes favor the induction of a secondary β-structure as characterized by spectroscopic analysis and fluorescence techniques. From this type of secondary structure, the formation of peptide aggregates and fibrillar structures with amyloid characteristics induced cytotoxicity in microglial cells in culture. These supramolecular structures promote cell cytotoxicity through the formation of reactive oxygen species (ROS) and change the balance of a series of proteins that control the process of endocytosis, similar to that observed when β-amyloid fibrils are employed. Therefore, a fine balance between the highly dynamic secondary structure of the C-terminal domain of CETP, the net charge, and the physicochemical characteristics of the surrounding microenvironment define the type of secondary structure acquired. Changes in this balance might favor misfolding in this region, which would alter the lipid transfer capacity conducted by CETP, favoring its propensity to substitute its physiological function.
Circulating MicroRNAs: Potential Biomarkers for Cancer
Authors: Yu De-Cai , Li Qing-Guo , Ding Xi-Wei , Ding Yi-Tao
Published: March 2011

Cancer is the leading cause of death in the world. Development of minimally invasive biomarkers for early detection of cancer is urgently needed to reduce high morbidity and mortality associated with malignancy. MicroRNAs (miRNAs) are small regulatory RNAs that modulate the activity of specific mRNA targets and play important roles in a wide range of physiologic and pathologic processes. Recently, miRNAs were found to be dysregulated in a variety of diseases including cancer. Emerging evidence suggests that miRNAs are involved in tumor initiation and progression. Together, the different expression profiles of miRNAs in cancer, and the stability of circulating miRNAs, make them new potentially clinical biomarkers for cancer diagnosis, classification, therapeutic decisions, and prognosis.
Cloning, Soluble Expression and Purification of High Yield Recombinant hGMCSF in Escherichia coli
Authors: Das Krishna M.P. , Banerjee Sampali , Shekhar Nivedita , Damodaran Karpagavalli , Nair Rahul , Somani Sandeep , Raiker Veena P. , Jain Shweta , Padmanabhan Sriram
Published: March 2011

Expression of human granulocyte macrophage colony stimulating factor (hGMCSF), a cytokine of therapeutic importance, as a thioredoxin (TRX) fusion has been investigated in Escherichia coli BL21 (DE3) codon plus cells. The expression of this protein was low when cloned under the T7 promoter without any fusion tags. High yield of GMCSF was achieved (~88 mg/L of fermentation broth) in the shake flask when the gene was fused to the E. coli TRX gene. The protein was purified using a single step Ni2+-NTA affinity chromatography and the column bound fusion tag was removed by on-column cleavage with enterokinase. The recombinant hGMCSF was expressed as a soluble and biologically active protein in E. coli, and upon purification, the final yield was ~44 mg/L in shake flask with a specific activity of 2.3 × 108 U/mg. The results of Western blot and RP-HPLC analyses, along with biological activity using the TF-1 cell line, established the identity of the purified hGMCSF. In this paper, we report the highest yield of hGMCSF expressed in E. coli. The bioreactor study shows that the yield of hGMCSF could be easily scalable with a yield of ~400 mg/L, opening up new opportunities for large scale production hGMCSF in E. coli.
In Vitro Ability of Currently Available Oximes to Reactivate Organophosphate Pesticide-Inhibited Human Acetylcholinesterase and Butyrylcholinesterase
Authors: Jun Daniel , Musilova Lucie , Musilek Kamil , Kuca Kamil
Published: March 2011

We have in vitro tested the ability of common, commercially available, cholinesterase reactivators (pralidoxime, obidoxime, methoxime, trimedoxime and HI-6) to reactivate human acetylcholinesterase (AChE), inhibited by five structurally different organophosphate pesticides and inhibitors (paraoxon, dichlorvos, DFP, leptophos-oxon and methamidophos). We also tested reactivation of human butyrylcholinesterase (BChE) with the aim of finding a potent oxime, suitable to serve as a “pseudocatalytic” bioscavenger in combination with this enzyme. Such a combination could allow an increase of prophylactic and therapeutic efficacy of the administered enzyme. According to our results, the best broad-spectrum AChE reactivators were trimedoxime and obidoxime in the case of paraoxon, leptophos-oxon, and methamidophos-inhibited AChE. Methamidophos and leptophos-oxon were quite easily reactivatable by all tested reactivators. In the case of methamidophos-inhibited AChE, the lower oxime concentration (10−5 M) had higher reactivation ability than the 10−4 M concentration. Therefore, we evaluated the reactivation ability of obidoxime in a concentration range of 10−3–10−7 M. The reactivation of methamidophos-inhibited AChE with different obidoxime concentrations resulted in a bell shaped curve with maximum reactivation at 10−5 M. In the case of BChE, no reactivator exceeded 15% reactivation ability and therefore none of the oximes can be recommended as a candidate for “pseudocatalytic” bioscavengers with BChE.
Physical Properties of Normal Grade Biodiesel and Winter Grade Biodiesel
Authors: Sadrolhosseini Amir Reza , Moksin Mohd Maarof , Nang Harrison Lau Lik , Norozi Monir , Yunus W. Mahmood Mat , Zakaria Azmi
Published: March 2011

In this study, optical and thermal properties of normal grade and winter grade palm oil biodiesel were investigated. Surface Plasmon Resonance and Photopyroelectric technique were used to evaluate the samples. The dispersion curve and thermal diffusivity were obtained. Consequently, the variation of refractive index, as a function of wavelength in normal grade biodiesel is faster than winter grade palm oil biodiesel, and the thermal diffusivity of winter grade biodiesel is higher than the thermal diffusivity of normal grade biodiesel. This is attributed to the higher palmitic acid C16:0 content in normal grade than in winter grade palm oil biodiesel.
(S)-(-)-Limonene Derivatives Containing (1H-1,2,3-Triazol-4-yl)methyl 4-Bromobenzoate
Authors: Valdomir Guillermo , Davyt Danilo
Published: March 2011

The synthesis of (S)-(-)-limonene derivatives containing (1H-1,2,3-triazol-4-yl)methyl 4-bromobenzoate were obtained by epoxidation, azidolysis and Huisgen’s 1,3-dipolar cycloaddition. Two products were isolated and characterized by FT-IR, 1H NMR, 13C NMR, ESI-MS and optical rotation.
Bond Behavior of Historical Clay Bricks Strengthened with Steel Reinforced Polymers (SRP)
Authors: Grande Ernesto , Imbimbo Maura , Sacco Elio
Published: March 2011

In the strengthening interventions of past and historical masonry constructions, the non-standardized manufacture processes, the ageing and the damage of masonry units, could significantly affect the properties of the surfaces where strengthening materials are applied. This aspect requires particular care in evaluating the performance of externally bonded strengthening layers, especially with reference to the detachment mechanism. The bond response of old masonries could be very different from that occurring in new masonry units which are the ones generally considered in most of the bond tests available in technical literature. The aim of the present paper is the study of the bond behavior of historical clay bricks strengthened with steel reinforced polymers (SRP) materials. In particular, the results of an experimental study concerning new manufactured clay bricks and old bricks extracted from different historical masonry buildings are presented. The obtained results, particularly in terms of bond resistance, detachment mechanism and strain distributions, are discussed for the purpose of analyzing the peculiarities of the historical bricks in comparison with new manufactured ones. Some considerations on the efficacy of the theoretical formulations of the recent Italian code are also carried out.
Astaxanthin: A Potential Therapeutic Agent in Cardiovascular Disease
Authors: Fassett Robert G. , Coombes Jeff S.
Published: March 2011

Astaxanthin is a xanthophyll carotenoid present in microalgae, fungi, complex plants, seafood, flamingos and quail. It is an antioxidant with anti-inflammatory properties and as such has potential as a therapeutic agent in atherosclerotic cardiovascular disease. Synthetic forms of astaxanthin have been manufactured. The safety, bioavailability and effects of astaxanthin on oxidative stress and inflammation that have relevance to the pathophysiology of atherosclerotic cardiovascular disease, have been assessed in a small number of clinical studies. No adverse events have been reported and there is evidence of a reduction in biomarkers of oxidative stress and inflammation with astaxanthin administration. Experimental studies in several species using an ischaemia-reperfusion myocardial model demonstrated that astaxanthin protects the myocardium when administered both orally or intravenously prior to the induction of the ischaemic event. At this stage we do not know whether astaxanthin is of benefit when administered after a cardiovascular event and no clinical cardiovascular studies in humans have been completed and/or reported. Cardiovascular clinical trials are warranted based on the physicochemical and antioxidant properties, the safety profile and preliminary experimental cardiovascular studies of astaxanthin.
Studies on the Genetic Variation of the Green Unicellular Alga Haematococcus pluvialis (Chlorophyceae) Obtained from Different Geographical Locations Using ISSR and RAPD Molecular Marker
Authors: Mostafa Noroozi , Omar Hishamuddin , Tan Soon Guan , Napis Suhaimi
Published: March 2011

Haematococcus pluvialis (Flotow) is a unicellular green alga, which is considered to be the best astaxanthin-producing organism. Molecular markers are suitable tools for the purpose of finding out genetic variations in organisms; however there have been no studies conducted on ISSR or RAPD molecular markers for this organism. The DNA of 10 different strains of H. pluvialis (four strains from Iran, two strains from Finland, one strain from Switzerland and three strains from the USA) was extracted. A genetic similarity study was carried out using 14 ISSR and 12 RAPD primers. Moreover, the molecular weights of the bands produced ranged from 0.14 to 3.4 Kb. The PCA and dendrogram clustered the H. pluvialis strains into various groups according to their geographical origin. The lowest genetic similarity was between the Iran2 and USA2 strains (0.08) and the highest genetic similarity was between Finland1 and Finland2 (0.64). The maximum numbers of bands produced by the ISSR and RAPD primers were 35 and 6 bands, respectively. The results showed that ISSR and RAPD markers are useful for genetic diversity studies of Haematococcus as they showed geographical discrimination.
Inter-Population Variability of Terpenoid Composition in Leaves of Pistacia lentiscus L. from Algeria: A Chemoecological Approach
Authors: Said Samir Ait , Fernandez Catherine , Greff Stéphane , Torre Franck , Derridj Arezki , Gauquelin Thierry , Mevy Jean-Philippe
Published: March 2011

Three different altitudes were selected to study the variability of terpenoid composition from leaves of female plants of Pistacia lentiscus L. throughout the elevation gradient. GC-MS analyses showed that terpenoid contents change with altitude. Forty nine compounds were identified with a high interpopulation variability for low- and midaltitude sites that also exhibited the same major components when data were expressed on dry weight basis. However, Two-Way-ANOVA followed by Tukey’s post hoc test showed that monoterpene hydrocarbons increased with elevation, giving values of 21.7, 37.5 and 221.5 µg g−1 dw for low- mid- and highlands, respectively. On the other hand, applying P.C.A. with data expressed in percentage of the chromatogram of the volatile extract led to the identification of three chemotypes associated with altitudinal levels. In highlands (Group I), the major compounds were b-caryophyllene (12%), δ-cadinene (9.3%) and a-pinene (6.3%) while in midlands (Group II), b-caryophyllene (11.5%), δ-cadinene (8.6%) and caryophyllene oxide (6.8%) were the main components. In lowlands (Group III) δ-cadinene (10.9%), cubebol (10.5%) and β-bisabolene (7.7%) were chiefly present. Hence, the involvement of genetic factors, temperature and drought in the chemical polymorphism of P. lentiscus associated with elevation is discussed in this report.
Challenges and Perspectives of Chemical Biology, a Successful Multidisciplinary Field of Natural Sciences
Authors: Rojas-Ruiz Fernando A. , Vargas-Méndez Leonor Y. , Kouznetsov Vladimir V.
Published: March 2011

Objects, goals, and main methods as well as perspectives of chemical biology are discussed. This review is focused on the fundamental aspects of this emerging field of life sciences: chemical space, the small molecule library and chemical sensibilization (small molecule microassays).
Molecular Characterization of Arabinoxylans from Hull-Less Barley Milling Fractions
Authors: Zheng Xueling , Li Limin , Wang Xiaoxi
Published: March 2011

Arabinoxylans were prepared from different hull-less barley milling fractions (bran, shorts and flour). The yields of hull-less bran arabinoxylan (HBB-AX), shorts arabinoxylan (HBS-AX) and flour arabinoxylan (HBF-AX) were 8.42%, 4.08% and 2.13% respectively. Sugar composition analysis showed that arabinose and xylose were the main sugars. HBF-AX had the highest Ara/Xyl ratio, followed by HBS-AX and HBB-AX. Size exclusion chromatography analysis (HPSEC) showed that HBF-AX had the highest molecular weight, followed by HBS-AX and HBB-AX, which had the lowest molecular weight. Aqueous solutions of HBB-AX, HBS-AX and HBF-AX had higher molecular weight(Mw) than in 0.5 mol/L NaOH and 1.0 mol/L NaOH. Dynamic light scattering analysis (DLS) showed that HBB-AX, HBS-AX and HBF-AX had existed in two states in distilled water, 0.5 mol/LNaOH, 1.0 mol/L NaOH, and DMSO/H2O (90/10), an unaggregated state and an aggregated state, with the latter predominating.
Molecular Approaches To Target GPCRs in Cancer Therapy
Authors: Innamorati Giulio , Valenti Maria Teresa , Giovinazzo Francesco , Dalle Carbonare Luca , Parenti Marco , Bassi Claudio
Published: March 2011

Hundreds of G protein coupled receptor (GPCR) isotypes integrate and coordinate the function of individual cells mediating signaling between different organs in our bodies. As an aberration of the normal relationships that organize cells’ coexistence, cancer has to deceive cell-cell communication in order to grow and spread. GPCRs play a critical role in this process. Despite the fact that GPCRs represent one of the most common drug targets, current medical practice includes only a few anticancer compounds directly acting on their signaling. Many approaches can be envisaged to target GPCRs involved in oncology. Beyond interfering with GPCRs signaling by using agonists or antagonists to prevent cell proliferation, favor apoptosis, induce maturation, prevent migration, etc., the high specificity of the interaction between the receptors and their ligands can be exploited to deliver toxins, antineoplastic drugs or isotopes to transformed cells. In this review we describe the strategies that are in use, or appear promising, to act directly on GPCRs in the fight against neoplastic transformation and tumor progression.
Efficient Gene Silencing by Self-Assembled Complexes of siRNA and Symmetrical Fatty Acid Amides of Spermine
Authors: Metwally Abdelkader A. , Pourzand Charareh , Blagbrough Ian S.
Published: March 2011

Gene silencing by siRNA (synthetic dsRNA of 21-25 nucleotides) is a well established biological tool in gene expression studies and has a promising therapeutic potential for difficult-to-treat diseases. Five fatty acids of various chain length and oxidation state (C12:0, C18:0, C18:1, C18:2, C22:1) were conjugated to the naturally occurring polyamine, spermine, and evaluated for siRNA delivery and gene knock-down. siRNA delivery could not be related directly to gene silencing efficiency as N4,N9-dierucoyl spermine resulted in higher siRNA delivery compared to N4,N9-dioleoyl spermine. GFP silencing in HeLa cells showed that the unsaturated fatty acid amides are more efficient than saturated fatty acid amides, with N4,N9-dioleoyl spermine resulting in the most efficient gene silencing in the presence of serum. The alamarBlue cell viability assay showed that fatty acid amides of spermine have good viability (75%–85% compared to control) except N4,N9-dilauroyl spermine which resulted in low cell viability. These results prove that unsaturated fatty acid amides of spermine are efficient, non-toxic, non-viral vectors for siRNA mediated gene silencing.
Spectroscopic Investigation of Composite Polymeric and Monocrystalline Systems with Ionic Conductivity
Authors: Radziuk Darya V. , Möhwald Helmuth
Published: March 2011

The conductivity mechanism is studied in the LiCF3SO3-doped polyethylene oxide by monitoring the vibrations of sulfate groups and mobility of Li+ ion along the polymeric chain at different EO/Li molar ratios in the temperature range from 16 to 90 °С. At the high EO/Li ratio (i.e., 30), the intensity of bands increases and a triplet appears at 1,045 cm−1, indicating the presence of free anions, ionic pairs and aggregates. The existence of free ions in the polymeric electrolyte is also proven by the red shift of bands in Raman spectra and a band shift to the low frequency Infra-red region at 65 < T < 355 °С. Based on quantum mechanical modeling, (method MNDO/d), the energies (minimum and maximum) correspond to the most probable and stable positions of Li+ along the polymeric chain. At room temperature, Li+ ion overcomes the intermediate state (minimum energy) through non-operating transitions (maximum energy) due to permanent intrapolymeric rotations (rotation of C, H and O atoms around each other). In solid electrolyte (Li2SO4) the mobility of Li+ ions increases in the temperature range from 20 to 227 °С, yielding higher conductivity. The results of the present work can be practically applied to a wide range of compact electronic devices, which are based on polymeric or solid electrolytes.
A Survey on Routing Protocols for Large-Scale Wireless Sensor Networks
Authors: Li Changle , Zhang Hanxiao , Hao Binbin , Li Jiandong
Published: March 2011

With the advances in micro-electronics, wireless sensor devices have been made much smaller and more integrated, and large-scale wireless sensor networks (WSNs) based the cooperation among the significant amount of nodes have become a hot topic. “Large-scale” means mainly large area or high density of a network. Accordingly the routing protocols must scale well to the network scope extension and node density increases. A sensor node is normally energy-limited and cannot be recharged, and thus its energy consumption has a quite significant effect on the scalability of the protocol. To the best of our knowledge, currently the mainstream methods to solve the energy problem in large-scale WSNs are the hierarchical routing protocols. In a hierarchical routing protocol, all the nodes are divided into several groups with different assignment levels. The nodes within the high level are responsible for data aggregation and management work, and the low level nodes for sensing their surroundings and collecting information. The hierarchical routing protocols are proved to be more energy-efficient than flat ones in which all the nodes play the same role, especially in terms of the data aggregation and the flooding of the control packets. With focus on the hierarchical structure, in this paper we provide an insight into routing protocols designed specifically for large-scale WSNs. According to the different objectives, the protocols are generally classified based on different criteria such as control overhead reduction, energy consumption mitigation and energy balance. In order to gain a comprehensive understanding of each protocol, we highlight their innovative ideas, describe the underlying principles in detail and analyze their advantages and disadvantages. Moreover a comparison of each routing protocol is conducted to demonstrate the differences between the protocols in terms of message complexity, memory requirements, localization, data aggregation, clustering manner and other metrics. Finally some open issues in routing protocol design in large-scale wireless sensor networks and conclusions are proposed.
Environmental Laws and Sustainability: An Introduction
Authors: Dernbach John C. , Mintz Joel A.
Published: March 2011

In this introduction to the special issue of Sustainability on environmental laws and sustainability, we attempt to synthesize key lessons from the issue’s ten substantive articles. These lessons involve the use of law to achieve integrated decision-making, the use of pre-existing laws to foster sustainability, the centrality of sub-national governments in achieving sustainability, the background law of unsustainable development, the growing importance of climate change, the need to use law to protect and restore ecological integrity, the importance of judicial review and nongovernmental organizations, the need to translate sustainability into specific legal principles, the challenge of creating an appropriate national legal structure for sustainability, the importance of sustainability assessment tools and institutions before and after laws are adopted, and the importance of “soft” law.
Sustainable Food Production Systems and Food Security: Economic and Environmental Imperatives in Yam Cultivation in Trelawny, Jamaica
Authors: Beckford Clinton , Campbell Donovan , Barker David
Published: March 2011

Members of the genus Dioscorea, food yams, were introduced to Jamaica from Africa during the slave era and have remained a staple in local diets and national cuisine. Yam cultivation has also been an important economic activity providing employment for thousands of rural Jamaicans. Until the 1960s yams were grown for local use by subsistence growers for home consumption or by commercial growers for sale in local produce markets. Since then, however, yam has also grown to become an important export crop. With its value added potential virtually untouched, this crop possesses intriguing possibilities from the standpoint of food security and rural livelihoods in yam growing areas of Jamaica. At the same time there are concerns about the ecological and economic sustainability of yam farming under current conditions. In this paper we will analyze the sustainability of yam cultivation and consider concrete strategies for increasing the environmental sustainability and enhancing its contribution to food security.
The Influence of Perception on the Distribution of Multiple Symmetries in Nature and Art
Authors: Van der Helm Peter A.
Published: March 2011

Much is already known about single mirror symmetry, but multiple mirror symmetry is still understood poorly. In particular, perceptually, multiple symmetry does not seem to behave as suggested by the number of symmetry axes alone. Here, theoretical ideas on single symmetry perception and their extensions to multiple symmetry are discussed alongside empirical findings on multiple symmetry perception. The evidence suggests that, apart from the number of axes, also their relative orientation is perceptually relevant. This, in turn, suggests that perception is responsible for the preponderance of 3-fold and 5-fold symmetries in flowers as well as for their absence in decorative art.
Asymmetric Organocatalytic Reactions of α,β-Unsaturated Cyclic Ketones
Authors: Dalpozzo Renato , Bartoli Giuseppe , Bencivenni Giorgio
Published: March 2011

The 1,4-conjugate addition of nucleophiles to α,β-unsaturated carbonyl compounds represents one fundamental bond-forming reaction in organic synthesis. The development of effective organocatalysts for the enantioselective conjugate addition of malonate, nitroalkane and other carbon and heteroatom nucleophiles to cycloenones constitutes an important research field and has been explored in recent years. At the same time, asymmetric Diels-Alder reactions have been developed and often a mechanism has been demonstrated to be a double addition rather than synchronous. This review aims to cover literature up to the end of 2010, describing all the different organocatalytic asymmetric 1,4-conjugate additions even if they are listed as transfer hydrogenation, cycloadditions or desymmetrization of aromatic compounds.
Mechanism of Diphtheria Toxin Catalytic Domain Delivery to the Eukaryotic Cell Cytosol and the Cellular Factors that Directly Participate in the Process
Authors: Murphy John R.
Published: March 2011

Research on diphtheria and anthrax toxins over the past three decades has culminated in a detailed understanding of their structure function relationships (e.g., catalytic (C), transmembrane (T), and receptor binding (R) domains), as well as the identification of their eukaryotic cell surface receptor, an understanding of the molecular events leading to the receptor-mediated internalization of the toxin into an endosomal compartment, and the pH triggered conformational changes required for pore formation in the vesicle membrane. Recently, a major research effort has been focused on the development of a detailed understanding of the molecular interactions between each of these toxins and eukaryotic cell factors that play an essential role in the efficient translocation of their respective catalytic domains through the trans-endosomal vesicle membrane pore and delivery into the cell cytosol. In this review, I shall focus on recent findings that have led to a more detailed understanding of the mechanism by which the diphtheria toxin catalytic domain is delivered to the eukaryotic cell cytosol. While much work remains, it is becoming increasingly clear that the entry process is facilitated by specific interactions with a number of cellular factors in an ordered sequential fashion. In addition,since diphtheria, anthrax lethal factor and anthrax edema factor all carry multiple coatomer I complex binding motifs and COPI complex has been shown to play an essential role in entry process, it is likely that the initial steps in catalytic domain entry of these divergent toxins follow a common mechanism.