bluuebunny/crossref_metadata_2025 huggingface dataset:
Correlation between miR-564, TGF-β1, and radiation-induced lung injury*
Authors: Ge Yunzhang , Xie Tao , Yang Bin , Li Qianxia , Ren Qingrong , Zhou Xiaoyi , Hu Desheng , Tu Zhongshu
Published: June 2023

Objective

Our study aimed to analyze the expression of miR-564 and TGF- β1 in cancer tissues and the serum of patients with radiation-induced lung injury, and to investigate the relationship between them and radiation-induced lung injury.

Methods

In situ hybridization and real-time fluorescence quantitative method were used to detect the expression of miR-564. Additionally, immunohistochemistry and enzyme-linked immunosorbent assay (ELISA) were performed to detect the expression of TGF- β1.

Results

The overall incidence of acute radiation pneumonia was 55.9% (100/179). The incidence of ≥ grade 2 radioactive pneumonia was 24.0% (43/179) and that of grade 1 was 31.8% (57/179). The expression of miR-564 in grade ≥ 2 was slightly higher than that in patients without or with grade 1, but there was no statistical difference (P= 0.86). The serum level and ratio of miR-564 in patients with grade ≥ 2 were significantly higher than those without or with grade 1 (P= 0.005,P= 0.025, respectively). The expression of TGF- β1 in grade ≥ 2 was significantly higher than that of patients without or with grade 1 (P= 0.017). The serum levels of TGF- β1 in grade ≥ 2 were significantly higher than those in patients without or with grade 1 (P= 0.038). Although the ratio of TGF- β1 in radiation pneumonia of grade ≥ 2 was significantly higher than that of without or with grade 1, there was no significant difference (P= 0.24). Moreover, patients with higher expression of miR-564 and lower expression of TGF-β1 had better prognosis.

Conclusion

MiR-564 and TGF-β1 are predictors of radiation-induced lung injury. Monitoring its changing trend can improve the accuracy of predicting radiation-induced lung injury. The levels and ratio of serum miR-564 and TGF- β1 in patients with radiation-induced lung injury are related to the severity of radiation-induced lung injury.
Condylar invasion after postoperative chemotherapy for primary intraosseous squamous cell carcinoma of the mandible: a case report
Authors: Cheng Long , Li Qinyun , Du Guobo , Ma Daiyuan , Tan Bangxian
Published: June 2023

Primary intraosseous squamous cell carcinoma (PIOSCC) is a rare malignant tumor that occurs predominantly in the jaw. Pathological evidence shows that PIOSCC originates from the residual odontogenic epithelium and a preexisting odontogenic cyst or tumor. Here, we report the case of a 63-year-old man with central squamous cell carcinoma of the mandible. On the basis of imaging and histopathology reports, the patient was diagnosed with PIOSCC of the jaw. Subsequently, he was treated with postoperative adjuvant chemotherapy. During a postoperative follow-up visit in the ninth month, mandibular computed tomography (CT) scanning and bone imaging revealed local recurrence and condylar invasion. Therefore, radiation along with chemotherapy was administered. This case study adds to the literature on PIOSCC and widens the understanding of its diagnosis, treatment, and prognosis.
Analysis of cancer incidence and mortality data in Heilongjiang province cancer registries, China, 2015
Authors: Sun Huixin , Song Bingbing , Zhang Maoxiang
Published: June 2023

Objective

In recent years, the rising incidence of cancer has increased patients' living and economic burdens. This study analyzed the incidence and death due to malignant tumors in tumor registries in Heilongjiang province (China) in 2015 to provide a scientific basis for the prevention and treatment of malignant tumors in this province.

Methods

Data on tumor incidence and patient deaths were collected from seven tumor registries in Heilongjiang province (China) in 2015. According to the stratification of urban and rural areas and patient sex, the crude, standard, and accumulative rates (0-74 years of age) were calculated. The 2000 China Population Census data and Segi's standard population were used to calculate the age-standardized rates.

Results

In 2015, the incidence rate of malignant tumors in Heilongjiang cancer registries was 259.90/100 000. The age-standardized incidence rates in the Chinese and world standard populations were 158.89/100 000 and 155.06/100 000, respectively, with a cumulative incidence rate (0-74 years) of 17.68%. The incidence of malignant tumors in urban areas was 273.55/100 000, while that in rural areas was 220.32/100 000. The incidence of malignant tumors in men was 270.89/100 000, higher than that in women (249.04/100 000). Lung cancer had the highest incidence, followed by breast cancer, liver cancer, colorectal cancer, and thyroid cancer. The mortality rate of malignant tumors in Heilongjiang cancer registries was 164.69/100 000. The age-standardized mortality rates in Chinese and in world standard populations were 95.29/100 000 and 94.35/100 000, respectively, with a cumulative mortality rate (0-74 years) of 10.44%. The mortality rate of malignant tumors in urban areas was 169.51/100 000, while that in rural areas was 150.72/100 000. The mortality rate of malignant tumors in men was 201.64/100 000, higher than that in women (128.21/100 000). Lung cancer had the highest mortality, followed by liver cancer, stomach cancer, colorectal cancer, and breast cancer.

Conclusion

Lung, liver, breast, and colorectal cancers were the most common cancers in Heilongjiang province, China, and should be considered the key cancer types for prevention and treatment. Moreover, the incidence of thyroid cancer is increasing, and thus early preventative measures should be implemented.
Radiation-induced brain injury after a conventional dose of intensity-modulated radiotherapy for nasopharyngeal carcinoma: a case report and literature review*
Authors: Zhang Qian , Tang Jie , Du Jiayu , Ma Xiaojie
Published: June 2023

A 61-year-old female nasopharyngeal carcinoma patient was admitted to the hospital with sudden cognitive dysfunction one month after Volumetric Intensity Modulated Arc Therapy (VMAT) conventional dose radiotherapy, and the initial diagnosis was radiation-induced brain injury (RBI). After comprehensive treatment with steroid hormones, the patient's condition rapidly improved. Typically, in nasopharyngeal carcinoma patients treated with VMAT, the incidence of RBI is extremely low when the temporal lobe dose is less than 65 Gy or 1% of the volume is less than 65 Gy. When this limit is exceeded, RBI may occur in varying degrees. However, in this case, even though the temporal lobe dose was under the prescribed limit, the patient still experienced RBI. The rare observations in this case can be used as a reference, and clinicians should seriously consider the possibility of RBI in similar cases.
Efficacy and adverse effects of olanzapine in the treatment of moderate to severe refractory neuropathic pain*
Authors: Dai Yuhong , Zou Man , Huang Tingting , Qiu Hong
Published: June 2023

Objective

The aim of the study was to investigate the efficacy and adverse effects of olanzapine in the treatment of moderate to severe refractory neuropathic pain.

Methods

Forty patients with digestive system cancer were enrolled, who had moderate to severe refractory neuropathic pain; the patients were treated with olanzapine for 2 weeks at a daily dosage of 5 mg to 10 mg per night according to patients' response and tolerability, combined with conventional analgesic therapy. Pain intensity was evaluated by using a Numeral Rating Scale (NRS) at baseline, 3 days, and 2 weeks after therapy. The Pittsburg Sleep Quality Index (PSQI) was evaluated at baseline and 2 weeks after therapy. Data on adverse events were recorded. The dosage of conventional analgesics was adjusted over time based on the severity of pain.

Results

The mean pain score decreased by 2.575 ± 1.318 (P< 0.000) at 3 days and by 3.400 ± 1.614 (P< 0.000) at 2 weeks; 30% of the patients experienced significant pain relief at 3 days and 50% at 2 weeks. The PSQI decreased by 4.725 ± 2.828 (P< 0.000) at 2 weeks. The adverse events induced by olanzapine included sleepiness, weight gain, dizziness, fatigue, dry mouth, and constipation; all the side effects were mild.

Conclusion

When combined with conventional analgesic therapy, olanzapine was effective in relieving pain and sleep disturbance, and was well-tolerated among patients with refractory neuropathic pain.
Effects of MIF on proliferation, migration, and STAT1 pathway of colon cancer cells
Authors: Liu Feng , Zhong Jianxin , Sun Jianbin , Wu Hailong
Published: June 2023

Objective

This study aimed to investigate how macrophage migration inhibitory factor (MIF) regulates the interaction of signal transducer and activator of transcription 1 (STAT1) with CD74, and affects colon cancer proliferation and invasion.

Methods

After transfecting MIF small interfering RNA into the SW480 cell line, the expression of STAT1 and CD74 mRNA was detected by qRT-PCR and western blotting. Transwell and MTT assays were performed to detect the colon cancer cell invasion and proliferation ability. Co-immunoprecipitation was used to detect the interaction between CD74 and STAT1 proteins in the treated and control groups.

Results

The cellular biological assays (MTT and Transwell) showed that the proliferation and invasion ability of colon cancer cells decreased after MIF knockdown; the results showed significant statistical difference (P < 0.05). The results of the co-immunoprecipitation assay suggested that MIF knockdown in colon cancer cells could inhibit the binding of CD74 and STAT1 proteins; statistical difference was observed between the two groups (P < 0.05).

Conclusion

MIF can increase the proliferation and invasion of colon cancer cells by promoting the combination of CD74 and STAT1.
The optional extent of lymph node dissection for pancreatic head cancer
Authors: Niu Guangcai , Ma Xiangdong
Published: June 2023

Objective

The extent of lymph node dissection for pancreatic head cancer (PHC) is uncertain and controversial; therefore, this study evaluated whether PHC patients can benefit from different extents of lymph node dissection.

Methods

A total of 106 PHC patients underwent standard regional lymphadenectomy (SRLN; n = 56, 52.8%) and extended regional lymphadenectomy (ERLN; n = 50, 47.2%) between September 2015 and September 2019. None of the study participants had distant metastases. The median survival time and complications were compared between the two groups.

Results

The median survival time in the SRLN and ERLN groups was 27.01 months and 21.17 months, respectively (P = 0.30). The postoperative major morbidity and mortality rates were 37.50% and 1.79% in the SRLN group, and 46.00% and 2.00% in the ERLN group, respectively. Moreover, the tumor differentiation, tumor diameter, lymph node involvement, perineural invasion, vascular invasion, and margin status all correlated with survival (P < 0.05).

Conclusion

For PHC patients, ERLN cannot provide a significant survival benefit over SRLN. Moreover, ERLN increased morbidity and mortality, although without statistical significance. This indicates that ERLN should not be considered in PHC patients.
Survival outcomes of patients with cervical esophageal cancer who received definitive radiotherapy: a retrospective study conducted in a single institution*
Authors: Wang Jing , Liu Fei , Wu Yingying , Zhou Lei , Hu Guangyuan , Yang Lin
Published: June 2023

Objective

Cervical esophageal cancer (CEC) is a relatively rare condition, with limited treatment options. The current study aimed to assess the survival outcomes of patients with CEC who received definitive radiotherapy.

Methods

In total, 63 consecutive patients with CEC who received definitive radiotherapy between 2010 and 2018 were included in this study. The survival outcomes were analyzed based on statistics.

Results

The median progression-free survival (PFS) and overall survival (OS) of the patients were 12 and 19 months, respectively. There were no significant differences in terms of survival outcomes between the groups who received radiation doses ≥ 60 and < 60 Gy. Interestingly, in the proximal CEC subgroup, the PFS (P= 0.039), OS (P= 0.031), and loco-regional failure-free survival (LRFFS) (P= 0.005) improved significantly in patients who received a radiation dose ≥ 60 Gy compared with those who received a radiation dose < 60 Gy. However, in the distal CEC subgroup, the PFS, OS, and LRFFS did not significantly improve between patients who received radiation doses ≥ 60 and < 60 Gy. Definitive radiotherapy was well tolerated, and no significant differences were observed in terms of treatment-related toxicities between the groups who received radiation doses ≥ 60 and < 60 Gy.

Conclusion

The survival outcomes of patients with CEC should be improved. In proximal CEC, a radiation dose ≥ 60 Gy is significantly correlated with better PFS, OS, and LRFFS. However, further research must be performed to validate this finding.
Expression and role of PTV1 lncRNA in glioma cells progression*
Authors: Li Yue , Bai Yuling , Qi Yan , Cai Chang , Liao Ying , Liu Xiuzhu , He Pengchen
Published: June 2023

Objective

The aim of this study was to investigate the expression of PTV1 lncRNA in gliomas and the mechanism of its interaction with miR-203a.

Methods

U87 and U251 cells were cultured stably and transfected with sh-PTV1 or ov-PTV1, respectively. The proliferative activity of U87 and U251 cells was detected and the transplanted tumor model nude mice were divided into U87 and U251 groups. U87-sh and u251-ov cells were injected into the armpit, then miR-203a mic and miR-203a inhibitors were administered to detect the changes in the expression of tumor-related proteins.

Results

The relative expression of PTV1 in gliomas was significantly higher than that in normal brain tissues, while in GBM it was significantly higher than that in low-grade gliomas. Knockdown of PTV1 significantly inhibited the proliferation of U87 cells, resulting in fewer cell clones; overexpression of rPTV1 significantly promoted the proliferation of U251 cells, resulting in more cell colonies. The dual Luciferase Reporter assay showed that SP2 was a potential target of miR-203a. When U87 cells were treated with a miR-203a mimic, the expression of SP2 decreased; and when U251 cells were treated with a miR-203a inhibitor, the expression of SP2 increased significantly. SP2 was overexpressed in u87-sh cells and the proliferation, migration, and invasion of u87-sh cells were significantly enhanced. U251-ov cells showed the opposite trend. Compared with the control group mice, the tumor volume in u87-sh group mice was significantly smaller and the positive rate of SP2 in tumor tissue was significantly lower. After administration of the miR-203a inhibitor, the tumor volume increased gradually and the positive rate of SP2 increased significantly, while u251-ov mice showed the opposite trend.

Conclusion

lncRNA PTV1 can be used as a molecule to interfere with miR-203a expression in order to downregulate SP2 and to promote the proliferation and invasion of glioma cells. lncRNA PTV1 may be a new biomarker and therapeutic target for glioma.
Expression of mir-34c-5p and mir-150-5p in nasopharyngeal carcinoma and up-regulated expression after invasion and apoptosis of nasopharyngeal carcinoma cells*
Authors: Zhou Xiaojun , Lin Baorui , Wang Yun
Published: June 2023

Objective

MiRNAs are closely related to tumors, and we hypothesized there is specific miR expression in nasopharyngeal carcinoma (NPC). We intended to investigate the expression of mir-34c-5p and mir-150-5p in NPC and to investigate the effects of mir-34c-5p and mir-150-5p on apoptosis and invasion following up-regulated expression in HNE1 NPC cells.

Methods

MiR-34c-5p and miR-150-5p expression levels in 30 individual cases of NPC and nasopharyngitis were detected with gene chip and qRT-PCR techniques. miR-34c-5p and miR-150-5p were transfected into the NPC cell line HNE1 via liposomes. Their expression levels were detected with qRT-PCR, apoptosis was evaluated by flow cytometry, and invasion ability was assessed via Transwell migration assay.

Results

MiR-150-5p expression levels in NPC and nasopharyngitis were 0.165 ± 0.092 and 1.062 ± 0.280 respectively, and miR-34c-5p expression levels in NPC and nasopharyngitis were 0.417 ± 0.220 and 1.385 ± 0.739, respectively, which indicated miR-34c-5p and miR-150-5p were weakly expressed in NPC. Apoptosis rates in HNE1 cells transfected by miR-34c-5p and miR-150-5p were increased, by 12.7% and 7.6%, respectively, which were significantly higher compared to blank control (3.9%). The Transwell assay demonstrated that invasive HNE1 cell counts were 32.00 ± 2.00 and 28.33 ± 2.08, respectively, compared to 60.66 ± 8.50 in the blank control (P < 0.001).

Conclusion

MiR-34c-5p and miR-150-5p are lowly expressed in NPC, and their down-regulation may be associated with NPC.
Megakaryocyte aplastic thrombocytopenia after CAR T-cell therapy in a patient with multiple myeloma: A case report
Authors: Liu Shangqin , Gui Chengsi
Published: June 2023

Chimeric antigen receptor (CAR) T-cell therapy is an effective new treatment strategy for hematologic malignancies. The success of CAR T-cell therapy in treating leukemia and lymphoma has promoted its development for multiple myeloma (MM), and the initial results of CAR T cell therapy have been encouraging. CAR T-cell therapy target antigens that have been clinically evaluated in MM; these antigens include CD19, B cell maturation antigen (BCMA), CD38, and CD138. A barrier to the widespread use of CAR T-cell therapy is its toxicity, primarily cytokine release syndrome (CRS), and neurologic toxicity. This study reports a patient with refractory MM who also developed megakaryocyte aplastic thrombocytopenia after receiving CAR T-cell therapy; such a case or the unusual side effects involving medications are yet unreported. There are risks in using cyclosporine and other immunosuppressants that may lead to MM recurrence as the use of such substances is contradictory to previous treatments; therefore, we temporarily administered platelet infusion as supportive care. Thus far, the condition of the patient has been steady and the patient regularly takes blood test in the hospital.
Efficacy and safety of berberine in the prophylactic treatment of acute radiation proctitis in postoperative patients with cervical cancer: a randomized controlled study*
Authors: Qin Kai , Cheng Yi , Yuan Xianglin , Zhang Jing , Huang Le
Published: June 2023

Objective

The aiom of this study was to study the efficacy and safety of berberine as a prophylactic treatment of acute radiation proctitis in postoperative patients with cervical cancer.

Methods

A total of 120 postoperative patients with cervical cancer were enrolled between July 2016 and October 2019, and randomly divided into a treatment group (berberine 300 mg three times a day, n = 60) and a control group (receiving vitamin C tablets, 100 mg three times a day; n = 60) using the random number table method. All patients received pelvic intensity-modulated radiation therapy (IMRT) and concurrent sensitizing chemotherapy weekly. The difference in the percentage of irradiation volume to the rectum and small intestine as well as the incidence, onset time, severity, and duration of acute radiation proctitis and cystitis during radiotherapy were compared between the two groups. The completion rate, completion time, number of chemotherapy sessions, and quality of life during radiotherapy were also compared.

Results

There were no statistical differences in age, FIGO stage, pathological type, complications, high-risk factors, and rectum and small intestine irradiation dose distribution (V20, V30, V40, and V50) between the two groups (P > 0.05). No acute radiation proctitis of grade 3 or above occurred in the two groups. There was no significant difference in the incidence of acute radiation cystitis, grade 2 acute radiation proctitis, completion rate of IMRT, and frequency of sensitization chemotherapy between the two groups. After prophylactic treatment with berberine, the incidence of grade 1 acute radiation proctitis, occurrence of grade 1 radiation proctitis, and completion time of radiotherapy in the treatment group were significantly lower than those in the control group (P < 0.05). The SF-36 score of the treatment group after radiotherapy was 67.53 ± 4.21, which was significantly better than that of the control group (64.90 ± 6.32; P < 0.05). The incidence of grade 3-4 neutropenia in the treatment group was 10% and lower than that in the control group (31.7%, P = 0.003). No adverse reactions related to berberine were observed.

Conclusion

Prophylactic prescription with oral berberine can reduce the incidence, onset time, and duration of grade 1 acute radiation proctitis, and improve the quality of life of postoperative patients with cervical cancer receiving concurrent chemoradiotherapy.
A case report of iodine-125 seed placement during operation for the treatment of advanced gallbladder carcinoma with septic shock*
Authors: Zhong Zhuo , Gao Fei , Lv Zhuo , Zhong Zhihui , Long De
Published: June 2023

This case report describes a patient with advanced gallbladder cancer who developed septic shock associated with iodine-125 (125I) seed implantation. The treatment process is described to provide a clinical reference for similar cases. A 52-year-old woman with recurrence of advanced gallbladder cancer underwent 125I seed implantation and developed postoperative sepsis with septic shock. The blood culture suggested infection with Aeromonas caviae and Enterococcus faecalis. Vancomycin and imipenem were immediately administered according to the drug sensitivity results, along with immunoglobulin therapy and vasoactive drugs. The patient’s condition gradually stabilized after comprehensive treatment. Sepsis with septic shock is a rare but potentially fatal complication of 125I seed implantation. Timely administration of broad-spectrum antibiotics, immunoglobulin therapy, and vasoactive drugs is very important to stabilize the patient’s condition. Our treatment of this patient can serve as a reference for clinicians to manage this complication in similar cases.
Construction and validation of an immune-related lncRNA prognostic model for rectal adenocarcinomas*
Authors: Jian Danni , Cheng Yi , Zhang Jing , Qin Kai
Published: June 2023

Objective

This study aimed to construct a prognostic model for rectal adenocarcinomas based on immune-related long noncoding RNAs (lncRNAs) and verify its prediction efficiency.

Methods

Transcript data and clinical data of rectal adenocarcinomas were downloaded from The Cancer Genome Atlas (TCGA) database. Perl software (strawberry version) and R language (version 3.6.1) were used to analyze the immune-related genes and immune-related lncRNAs of rectal adenocarcinomas, and the differentially expressed immune-related lncRNAs were screened according to the criteria |log2FC| > 1 and P < 0.05. The key immune-related lncRNAs were screened using single-factor Cox regression analysis and lasso regression analysis. Multivariate Cox regression analysis was performed to construct an immune-related lncRNA prognostic model using the risk scores. Next, we evaluated the effectiveness of the model through Kaplan-Meier (K-M) survival analysis, ROC curve analysis, and independent prognostic analysis of clinical features. In addition, prognostic biomarkers of immune-related lncRNAs in the model were analyzed by K-M survival analysis.

Results

In this study, we obtained gene expression profile matrices of 89 rectal adenocarcinomas and 2 paracancerous specimens from TCGA database and applied immunologic signatures to these transcripts. Through R and Perl software analysis, we obtained 847 immune-related lncRNAs and 331 protein-encoded immune-related genes in rectal adenocarcinomas. Eight important immune-related lncRNAs related to the prognosis of rectal adenocarcinomas were identified using univariate Cox regression and lasso regression analysis. Furthermore, four immune-related lncRNAs were identified as prognostic markers of rectal adenocarcinomas via multivariate Cox regression analysis. The prognostic risk model was as follows: risk score = (-4.084) * expression LINC01871 + (3.112) * expression AL158152.2 + (7.616) * expression PXN-AS1 + (-0.867) * expression HCP5. The independent prognostic effect of the rectal adenocarcinoma risk score model was revealed through K-M analysis, ROC curve analysis, and univariate, and multivariate Cox regression analysis (P = 0.035). LINC01871 (P = 0.006), PXN-AS1 (P = 0.008), and AL158152.2 (P = 0.0386) were closely correlated with the prognosis of rectal adenocarcinomas through the K-M survival analysis.

Conclusion

We constructed a prognostic model of rectal adenocarcinomas based on four immune-related lncRNAs by analyzing the data based on TCGA database, with high prediction accuracy. We also identified two biomarkers with poor prognosis (PXN-AS1 and AL158152.2) and one biomarker with good prognosis (LINC01871).
The clinical efficacy of percutaneous ethanol-lipiodol injection (PEI) combined with high-intensity focused ultrasound (HIFU) for small hepatocellularcarcinoma in special or high-risk locations*
Authors: Zou Xiaoli , Zhao Changzhi , Wang Tao , Jia Li , Feng Zhongyi , Wang Xiaoguang , Wei Lei , Liu Xiaobei
Published: June 2023

Objective

The objective of this study was to explore the short-term effects and postoperative complications of ultrasound-guided percutaneous ethanol-lipiodol injection (PEI) combined with high-intensity focused ultrasound (HIFU) for the treatment of small hepatocellular carcinoma in a special or high-risk location.

Methods

Forty patients with small liver cancer in a special or high-risk location were randomly divided into two groups: 20 patients were treated with PEI combined with HIFU (P + H group), and 20 patients were treated with HIFU alone (H group). There were no significant differences in average age, liver function, tumor location, tumor number, or tumor size between the two groups (P > 0.05).

Results

Significant differences were observed in ablation parameters between the two groups (P < 0.05). Under the same power, ablation rates of the P + H group were significantly higher than those in the H group, and postoperative complications in the P + H group were significantly lower than those in the H group (P < 0.05).

Conclusion

The combination of PEI and HIFU has better clinical value than HIFU alone for small-cell liver cancer in special or high-risk locations.
KIF15 expression characteristics: Relevance toneo-adjuvant chemotherapy efficacy in breast cancer*
Authors: Dong Mengting , Li Chunyang , Jiapaer Patiguli , Li Xiaofei , Xue Xiaohong , Jiang Ke , Sheng Jiayu
Published: June 2023

Objective

The relationship between the expression of kinesin family member 15 (KIF15) and clinicopathological features in breast cancer (BC) remains controversial. In this study, we aimed to explore the influence of KIF15 expression on the efficacy of neoadjuvant chemotherapy (NAC) and evaluate its clinical value in predicting prognosis for BC patients.

Methods

Immunohistochemistry was used to detect KIF15 expression in 93 BC patients undergoing NAC to analyze the relationship between KIF15 expression and clinical efficacy and analytical parameters.

Results

Of the 93 BC patients enrolled, 24.73% who underwent NAC had higher KIF15 expression levels, showing positive correlations with ER, HER-2, Ki67, and lymph node metastasis (P < 0.05). The clinical benefit of NAC was 70.97%, and the major histological response (MHR) rate was 61.29%. The effective therapeutic rate in patients with high KIF15 expression was 95.65%, while the MHR rate was 65.22%. Various molecular BC subtypes with varied clinical and pathological responses exhibited correlation to a large extent. Of all the BC patients studied, 84% of the triple-negative breast cancer (TNBC) patients were evaluated as clinically effective, and 52% of the TNBC patients were evaluated as pathologically effective, and these values were significantly higher than those of the other molecular types (P < 0.05). The expression of KIF15 in 25 TNBC patients showed positive correlations with lymph node metastasis.

Conclusion

Overexpression of KIF15 was shown to increase BC sensitivity to chemotherapy and demonstrated better outcomes.
Mechanism of tumor synthetic lethal-related targets
Authors: Zhang Yuhang , Xu Peng
Published: June 2023

Synthetic lethality is becoming more and more important in the precise treatment of oncology. Malignant tumors caused by gene mutations involve a complex DNA signaling process, and inhibition of DNA signaling in different ways may more effectively control the occurrence and development of tumors. Inhibition of tumor paired lethal genes effectively kills tumor cells, and more and more novel drugs that inhibit tumors are developing in this direction. This article reviews the synthetic lethal theory and discusses selection of drugs to target mutated genes in common solid tumors. The synthetic lethal gene pairs, representative targeted drugs, and related characteristics of four tumor types: lung cancer, breast cancer, colon cancer and prostate cancer, are systematically reviewed.
Identification of microRNA-21 as a valuable diagnostic marker of oral squamous cell carcinoma and potential target*
Authors: Yang Hua , Wei Yuxue , Liu Gangli
Published: June 2023

Objective

The aim of the study was to summarize the diagnostic value of miR-21 as a biomarker in oral squamous cell carcinoma (OSCC) using a review of the literature and data from the cancer genome atlas (TCGA) database.

Methods

Data from TCGA database was sorted and analyzed by bioinformatics to determine the expression level of miR-21 in OSCC. Further, we searched for relevant articles in Embase, PubMed/Medline, Scopus, and Web of Science published before March 2021, extracted the data, and conducted quality assessment. The bivariate meta-analysis model with Stata 16.0 was used to analyze the diagnostic value of miR-21 for OSCC.

Results

A total of 304 related articles were identified, and seven were selected for meta-analysis. The diagnostic results after analysis were as follows: sensitivity 0.76 [95% confidence interval (CI), 0.57-0.88]; specificity 0.77 (95% CI, 0.58-0.89); positive likelihood ratio 3.34 (95% CI, 1.58-7.08); negative likelihood ratio 0.31 (95% CI, 0.15-0.63); diagnostic odds ratio 10.75 (95% CI, 2.85-40.51); and area under the curve 0.83 (95% CI, 0.80-0.86). The Deeks’ funnel chart showed that there was no potential bias (P = 0.54). Prediction analysis of the potential target genes of miR-21 was performed via the biological website, and DAVID was used to cross target genes for gene ontology (GO) annotation function analysis.

Conclusion

The results showed that miR-21-3p and miR-21-5p were significantly more highly expressed in OSCC tissues than in normal tissues (P < 0.05), and the results of the meta-analysis indicated that they could be used as potential biomarkers in the diagnosis of OSCC. In addition, 58 potential target genes of miR-21 were significantly enriched in 28 GO annotation functional pathways, which provided a biological basis for further clinical diagnostic value research.
Cone beam computed tomography-guided differences among registration methods for lung cancer and the effects of tumor position, treatment model, and tumor size on positioning errors*
Authors: Du Jiayu , Tang Jie , Zhang Qian , Ma Xiaojie
Published: June 2023

Objective

To explore the differences in three different registration methods of cone beam computed tomography (CBCT)-guided down-regulated intense radiation therapy for lung cancer as well as the effects of tumor location, treatment mode, and tumor size on registration.

Methods

This retrospective analysis included 80 lung cancer patients undergoing radiotherapy in our hospital from November 2017 to October 2019 and compared automatic bone registration, automatic grayscale (t + r) registration, and automatic grayscale (t) positioning error on the X-, Y-, and Z-axes under three types of registration methods. The patients were also grouped according to tumor position, treatment mode, and tumor size to compare positioning errors.

Results

On the X-, Y-, and Z-axes, automatic grayscale (t + r) and automatic grayscale (t) registration showed a better trend. Analysis of the different treatment modes showed differences in the three registration methods; however, these were not statistically significant. Analysis according to tumor sizes showed significant differences between the three registration methods (P < 0.05). Analysis according to tumor positions showed differences in the X- and Y-axes that were not significant (P > 0.05), while the autopsy registration in the Z-axis showed the largest difference in the mediastinal and hilar lymph nodes (P < 0.05).

Conclusion

The treatment mode was not the main factor affecting registration error in lung cancer. Three registration methods are available for tumors in the upper and lower lungs measuring < 3 cm; among these, automatic gray registration is recommended, while any gray registration method is recommended for tumors located in the mediastinal hilar site measuring < 3 cm and in the upper and lower lungs ≥ 3 cm.
GFPT2 pan-cancer analysis and its prognostic and tumor microenvironment associations*
Authors: Zhang Jiachen , Wang Ting , Wei Siang , Chen Shujia , Bi Juan
Published: June 2023

Objective

Glutamine fructose-6-phosphate transaminase 2 (GFPT2) is involved in a wide range of biological functions in human cancer. However, few studies have comprehensively analyzed the correlation between GFPT2 and different cancer prognoses and tumor microenvironments (TMEs).

Methods

We evaluated the expression level and prognostic value of GFPT2 using updated public databases and multiple comprehensive bioinformatics analysis methods and explored the relationship between GFPT2 expression and immune infiltration, immune neoantigens, tumor mutational burden (TMB), and microsatellite instability in pan-cancer.

Results

GFPT2 was highly expressed in five cancers. GFPT2 expression correlates with the prognosis of several cancers from The Cancer Genome Atlas (TCGA) and is significantly associated with stromal and immune scores in pan-cancer. High GFPT2 expression in BLCA, BRCA, and CHOL was positively correlated with the infiltration of immune cells, such as B-cells, CD4+ T, CD8+ T cells, dendritic cells, neutrophils, and macrophages.

Conclusion

High GFPT2 expression may modify the outcomes of patients with BLCA, BRCA, or CHOL cancers by increasing immune cell infiltration. These findings may provide insights for further investigation into GFPT2 as a potential target in pan-cancer.
Special IgD-λ type multiple myeloma based on bone marrow cell morphology: A case report
Authors: Zhang Xiaofang , Zhang Yanqing , Zhang Yungang , Wan Chuanke , Xu Ling , Li Ruimin
Published: June 2023

We aimed to explore the changes of laboratory indexes of IgD- λ type multiple myeloma with special cell morphology, and to improve the cognition of IgD-λ type MM. To explore the changes of laboratory indexes of IgD-λ type 1 multiple myeloma with special cell morphology, and to improve the cognition of IgD-λ type MM. The morphology of bone marrow cells, immunofixation electrophoresis, serum free light chain (sFLC) and other detection indexes of a patient with IgD-λ type MM treated in Handan Central Hospital in December 2020 were analyzed. The patient bone marrow smears showed 62% of abnormal cells-which were distributed in clusters and resembled lymphoma and metastatic cancer cells. The Flowcytometry indicates that the cell is a plasma cell tumor.Immunoglobulin IgG, IgA and IgM were all lower than the normal range.There is a monoclonal light chain λ component in immunofixation electrophoresis.The serum free light chain λ was 2700.00 mg/ L, light chain k/λ is 0.0023, the high of serum calcium, LDH, β2 microglobulin. IgD-λ type MM is a rare type of MM. The age of onset is young, the invasiveness is strong, the prognosis is poor, the clinical manifestation is complex, and it is easy to be misdiagnosed or missed. The analysis of the clinical symptoms and laboratory characteristics of the disease plays a positive role in the diagnosis, treatment and prognosis of the disease.
Gene mutation analysis and immune checkpoint therapy in head and neck squamous cell carcinoma*
Authors: Yang Hua , Wei Yuxue , Liu Gangli
Published: June 2023

Immune checkpoint inhibitors (ICI), represented by blocked programmed cell death-1 (PD-1), is a group of novel medicines for anti-tumor immunotherapy. It has been approved by the U.S. Food and Drug Administration (FDA) in recent years for relapsed or metastatic head and neck squamous cell carcinoma (HNSCC), and brings promising treatment prospects. However, the instability caused by tumor gene mutations significantly compromises the therapeutic effect of ICI. Therefore, the identification and analysis of HNSCC gene mutations can further guide and optimize the application of ICIs in HNSCC. In this study, we preliminarily described the clinical research progress of ICI therapy and the potential immune escape mechanism in HNSCC. An overview of complete HNSCC gene mutation results was generated from the bioinformatics study of TCGA database to further explain and analyze the relevant molecular mechanisms, which may aid in designing future personalized therapeutic strategies for HNSCC patients.
Prognostic value of serum carcinoembryonic antigen combined with nutritional status control score in patients with colorectal cancer*
Authors: Zhang Yichao , Wang Biao , Zhang Yongchuan , Xiong Gang , Pang Xiao
Published: June 2023

Objective

To investigate the prognostic value of serum carcinoembryonic antigen (CEA) and controlling nutritional status (CONUT) score in patients with colorectal cancer.

Methods

We retrospectively studied 261 patients with colorectal cancer in our hospital. The patients were divided into two groups by CONUT = 3 and CEA = 5 ng/mL, and the effects of CONUT score and CEA level on the prognosis and clinicopathological parameters were statistically analyzed.

Results

(1) Different CONUT scores were significantly correlated with age, tumor diameter, differentiation type, and T stage (P < 0.05). The older the patient was, the larger the tumor diameter, undifferentiated tumor, and T stage were, the higher the CONUT score was. (2) Seventy-five patients died during the follow-up period, and 45 patients died of progression or recurrence of colorectal cancer. The 5-year overall survival (OS) rate of the low CONUT score group was significantly higher than that of the high CONUT score group, and the 5-year OS rate of the low CEA group was significantly higher than that of the high CEA group; the difference was statistically significant (P < 0.01). (3) According to the serum CEA level and CONUT score, the 5-year survival rates of CEAlow/CONUTlow, CEAlow/CONUThigh, CEAhigh/CONUTlow, and CEAhigh/CONUThigh were 84.7%, 69%, 55.3%, and 36.1% respectively, with statistical significance (P <0.01). (4) The Cox multivariate analysis showed that age, CONUT score, CEA combined with CONUT score, lymph node metastasis, and distant metastasis were independent risk factors for the prognosis of colorectal cancer patients.

Conclusion:

The combination of CEA detection and CONUT score can more accurately judge the prognosis of colorectal cancer patients.
Spindle epithelial tumor with thymus-like differentiation: A case report and literature review*
Authors: Zheng Li , Wang Jin , Ang Lin , Huang Jin , Zhao Min
Published: June 2023

Spindle epithelial tumor with thymus-like differentiation (SETTLE) is a rare, low-grade malignant neoplasm that mainly occurs in the thyroid gland or its perimeter. Fewer than 10 cases of SETTLE have been reported in the Chinese literature, whereas approximately 50 cases have been reported in the international literature. We report a rare case of SETTLE that occurred in the soft tissues of the neck around the thyroid gland in a 41-year-old man. The neoplastic tissue, which mainly consisted of spindle epithelial components, included sporadically visible thymus-like structures. Immunohistochemistry demonstrated CK and CK19 positivity in the thymic epithelial components and CK, vimentin, and Bcl-2 positivity in the spindle cells. Surgical resection is the main treatment option for SETTLE. This patient underwent mass resection of the neck, and no recurrence or metastasis was observed even at 8 months postoperatively.
A metabolism-associated gene signature with prognostic value in colorectal cancer*
Authors: Xiao Lingyan , Huang Yongbiao , Qin Wan , Liu Chaofan , Qiu Hong , Liu Bo , Yuan Xianglin
Published: June 2023

Objective

In this study, our goal was to explore the role of metabolism-associated genes in colorectal cancer (CRC) and construct a prognostic model for patients with CRC.

Methods

Differential expression analysis was conducted using RNA-sequencing data from The Cancer Genome Atlas (TCGA) dataset. Enrichment analyses were performed to determine the function of dysregulated metabolism-associated genes. The protein-protein interaction (PPI) network, Kaplan-Meier curves, and stepwise Cox regression analyses identified key metabolism-associated genes. A prognostic model was constructed using LASSO Cox regression analysis and visualized as a nomogram. Survival analyses were conducted in the TCGA and Expression Omnibus (GEO) cohorts to demonstrate the predictive ability of the model.

Results

A total of 332 differentially expressed metabolism-associated genes in CRC were screened from the TCGA cohort. Differentially expressed metabolism-associated genes mainly participate in the metabolism of nucleoside phosphate, ribose phosphate, lipids, and fatty acids. A PPI network was constructed out of 328 key genes. A prognostic model was established based on five prognostic genes (ALAD, CHDH, ISYNA1, NAT1, and P4HA1) and was demonstrated to predict survival in the TCGA and GEO cohorts accurately.

Conclusion

The metabolism-associated prognostic model can predict the survival of patients with CRC. Our work supplements previous work focusing on determining prognostic factors of CRC and lays a foundation for further mechanistic exploration.
Relationship between IKZF1 polymorphisms and the risk of acute lymphoblastic leukemia: a meta-analysis*
Authors: Wang Sisi , Lin Chuyang , Xi Tingting , Tong Yu , Wu Jinlin
Published: June 2023

Objective

The aim of the study was to systematically evaluate the correlation between IKZF1 polymorphisms and the risk of acute lymphoblastic leukemia.

Methods

Computer databases including PubMed, EMBASE, and Web of Science were searched for case-control studies on the association between IKZF1 polymorphisms and the risk of acute lymphoblastic leukemia. The retrieval period was from the establishment of the database to November 2020. Two researchers independently screened the literature, extracted the data, evaluated the risk of bias in the included studies, and used Stata 14.0 software for meta-analysis.

Results

A total of 48 case-control studies were included, with 10 520 and 44 049 cases in the case and control groups, respectively. The meta-analysis results showed that rs4132061 and rs11978267 of IKZF1 were significantly correlated with the risk of acute lymphoblastic leukemia (ALL).

Conclusion

Current evidence indicates that rs4132061 and rs11978267 of IKZF1 are significantly associated with the risk of B-cell ALL.
CD14 macrophage and IL-10 levels in the peripheral blood of breast cancer patients and their diagnostic value*
Authors: Dong Mengting , Sheng Jiayu , Li Chunyang , JIAPAER Patiguli , Li Xiaofei , Yuan Minjia , Xue Xiaohong , Jiang Ke
Published: June 2023

Objective

To explore the correlation between macrophages and interleukin-10 (IL-10 in the peripheral blood of breast cancer (BC) patients and the diagnostic value of joint detection.

Methods

BC patients (n = 50) and healthy controls (n = 40) were prospectively recruited. The percentage of circulating cluster of differentiation 14 (CD 14) macrophage cells was analyzed by flow cytometry, and an enzyme-linked immunosorbent assay (ELISA) was used to detect IL-10 expression levels. Receiver operating characteristic (ROC) curves were used to verify the diagnostic value of the models based on the expression of CD14 macrophage cell populations and IL-10. In addition, the association between model expression and clinicopathological characteristics was investigated. Another 30 patients with BC and 30 with benign breast disease were selected to validate the IL-10 and CD14 macrophage joint detection model using the same method.

Results

CD14 macrophage and IL-10 expression levels in BC patients were higher than those in healthy controls (P < 0.05). The ROC curve showed that the area under the curve (AUC) of CD14+ macrophages combined with IL-10 was 0.830, the sensitivity was 72.0%, and the specificity was 87.5%. Its diagnostic efficiency was better than all other single and joint detections. Correlation analysis of clinicopathological features showed that IL-10 and CD14+ macrophage joint detection was significantly correlated with tumor size, tumor-node-metastasis (TNM) stage, and lymph node, estrogen receptor (ER), and Ki-67 expression (P < 0.05). The validation analysis results were consistent with the test results.

Conclusion

Peripheral blood macrophages can be an independent diagnostic marker for BC. Joint detection of CD14- macrophages and IL-10 suggests poor prognosis, which has unlimited potential to guide BC development and provides a new theory for studying tumor-associated macrophages in BC.
The application study of dual-energy CT nonlinear blending technique in pulmonary angiography*
Authors: Yi Siqi , Zhou Peng , He Yakun , He Changjiu , Hu Shibei
Published: June 2023

Objective

This study aimed to explore the feasibility of enhancing image quality in computed tomography (CT) pulmonary angiography (CTPA) and reducing radiation dose using the nonlinear blending (NLB) technique of dual-energy CT.

Methods

A total of 61 patients scheduled for CTPA were enrolled, and 30 patients underwent dual-energy scanning. Nonlinear blending images (NLB group) and three groups of linear blending images (LB group, 80 kV group, and 140 kV group) were reconstructed after scanning; 31 patients underwent single-energy scanning (120 kV group). The CT values and standard deviations of the pulmonary trunk, left and right pulmonary arteries, and ipsilateral back muscle at the bifurcation level of the left and right pulmonary arteries were measured. The signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR) of the five groups were calculated. The subjective image quality of the five groups was assessed. The radiation doses of the dual- and single-energy groups were recorded and calculated.

Results

The CNR and SNR values of blood vessels in the NLB group were significantly higher than those in the LB, 140 kV, and 80 kV groups (CNR of pulmonary artery trunk: t = 3.50, 4.06, 7.17, all P < 0.05; SNR of pulmonary trunk: t = 3.76, 4.71, 6.92, all P < 0.05). There were no statistical differences in the CNR and SNR values between the NLB group and 120 kV group (P > 0.05). The effective radiation dose of the dualenergy group was lower than that of the single-energy group (t = -4.52, P < 0.05). The subjective scores of images in the NLB group were the highest (4.28 ± 0.74).

Conclusion

The NLB technique of dual-energy CT can improve the image quality of CTPA and reduce the radiation dose, providing more reliable imaging data for the clinical diagnosis of pulmonary embolism.