bluuebunny/crossref_metadata_2025 huggingface dataset:
Modeling Primary Emissions of Chemicals from Liquid Products Applied on Indoor Surfaces
Authors: Wei Wenjuan , Little John C. , Nicolas Mélanie , Ramalho Olivier , Mandin Corinne
Published: August 2022

Liquid products applied on material surfaces and human skin, including many household cleaning products and personal care products, can lead to intermittent emissions of chemicals and peak concentrations in indoor air. The existing case-based models do not allow inter-comparison of different use scenarios and emission mechanisms. In this context, the present work developed a mechanistic model based on mass transfer theories, which allowed emissions into the air from the liquid product to be characterized. It also allowed for diffusion into the applied surface during product use and re-emission from the applied surface after the depletion of the liquid product. The model was validated using literature data on chemical emissions following floor cleaning and personal care product use. A sensitivity analysis of the model was then conducted. The percentage of the chemical mass emitted from the liquid to the air varied from 45% (applied on porous material) to 99% (applied on human skin), and the rest was absorbed into the applied material/skin. The peak gas-phase concentration, the time to reach the peak concentration, and the percentage of the liquid-to-air emission depended significantly on the chemical’s octanol/gas and material/gas partition coefficients and the diffusion coefficient of the chemical in the applied material/skin.
Importance of Public Transport Networks for Reconciling the Spatial Distribution of Dengue and the Association of Socio-Economic Factors with Dengue Risk in Bangkok, Thailand
Authors: Lefebvre Bertrand , Karki Rojina , Misslin Renaud , Nakhapakorn Kanchana , Daudé Eric , Paul Richard E.
Published: August 2022

Dengue is the most widespread mosquito-borne viral disease of man and spreading at an alarming rate. Socio-economic inequality has long been thought to contribute to providing an environment for viral propagation. However, identifying socio-economic (SE) risk factors is confounded by intra-urban daily human mobility, with virus being ferried across cities. This study aimed to identify SE variables associated with dengue at a subdistrict level in Bangkok, analyse how they explain observed dengue hotspots and assess the impact of mobility networks on such associations. Using meteorological, dengue case, national statistics, and transport databases from the Bangkok authorities, we applied statistical association and spatial analyses to identify SE variables associated with dengue and spatial hotspots and the extent to which incorporating transport data impacts the observed associations. We identified three SE risk factors at the subdistrict level: lack of education, % of houses being cement/brick, and number of houses as being associated with increased risk of dengue. Spatial hotspots of dengue were found to occur consistently in the centre of the city, but which did not entirely have the socio-economic risk factor characteristics. Incorporation of the intra-urban transport network, however, much improved the overall statistical association of the socio-economic variables with dengue incidence and reconciled the incongruous difference between the spatial hotspots and the SE risk factors. Our study suggests that incorporating transport networks enables a more real-world analysis within urban areas and should enable improvements in the identification of risk factors.
Does Shiftwork Impact Cognitive Performance? Findings from the Canadian Longitudinal Study on Aging (CLSA)
Authors: Alonzo Rea , Anderson Kelly K. , Rodrigues Rebecca , Klar Neil , Chiodini Paolo , Montero-Odasso Manuel , Stranges Saverio
Published: August 2022

Few large nationwide studies have investigated the relationship between shiftwork and cognitive performance, and little is known about whether and how psychological distress may impact this relationship. This study aimed to examine: (1) the cross-sectional relationship between shiftwork (yes/no) and some aspects of cognitive performance (declarative memory and executive functioning) and (2) the potential moderating effect of psychological distress among 20,610 community-dwelling adults from the comprehensive cohort of the Canadian Longitudinal Study on Aging (CLSA). Differences by sex and retirement status were also explored. Shiftwork was significantly associated with poorer performance for executive functioning (interference condition: ß = 0.47, 95% CI: 0.31 to 0.63; MAT: ß = −0.85, 95% CI: −1.21 to −0.50) but not for declarative memory. Completely and not/partly retired males showed poorer cognitive performance on executive functioning. However, no evidence of a moderating effect by psychological distress was found. Our findings confirm the association between shiftwork and cognitive performance and highlight important health correlates of shiftwork.
Nurses’ Perception of Patient Safety Culture in a Referral Hospital: A Cross-Sectional Study
Authors: Sosa-Palanca Eva María , Saus-Ortega Carlos , Gea-Caballero Vicente , Andani-Cervera Joaquín , García-Martínez Pedro , Ortí-Lucas Rafael Manuel
Published: August 2022

Healthcare systems are becoming increasingly complex which is helping to promote a ‘culture of safety’ within them based on the best scientific evidence available. Indeed, creating a positive institutional culture of patient safety is reflected in health outcomes. The aim of this present study was to describe the perception of culture of safety by nurses in adult inpatient units in a tertiary hospital and to analyze adverse events reporting. It was a cross-sectional study in which 202 nurses from adult hospitalization units of the Hospital Universitario y Politécnico La Fe in Valencia (Spain) participated. The perception of safety culture was measured using the Hospital Survey on Patient Safety questionnaire version 1.0, which consists of 42 items distributed in 12 dimensions that are considered strengths or weaknesses. In addition, adverse events related to nursing care during the study period and those reported in the official hospital registry were collected. Finally, the association between safety culture and sociodemographic and labor variables was explored. A total of 148 responses to the questionnaire were analyzed (Cronbach’s alpha = 0.94), where seven dimensions and 25 items were identified as weaknesses. Two hundred and fourteen events were identified and none were reported in the official registry. Years of experience were significantly (p < 0.05) associated with safety culture. It is necessary to establish strategies to improve the perception of the safety culture of nurses, as well as to make nurses aware of the importance of notifying adverse events derived from health care.
Effects of Self-Rated Health Status on Residents’ Social-Benefit Perceptions of Urban Green Space
Authors: Tian Yuhong , Liu Fenghua , Jim Chi Yung , Wang Tiantian , Luan Jingya , Yan Mengxuan
Published: August 2022

Urban green spaces (UGS) provide many social benefits and improves residents’ wellbeing. Studying residents’ perceptions of UGS’s social benefits and driving factors could promote public health and environmental justice. A questionnaire survey of 432 Beijing residents and statistical tests assessed the impacts of residents’ living environments and self-rated health status on UGS perceptions. The results showed: (1) perceptions of UGS’ physical health benefits were subdued, with an inclination towards other social benefits. Respondents more highly perceived accelerating patient recovery and reducing morbidity and mortality rates. Perceptions of bearing larger-head babies with higher weight were relatively low. For other social benefits, perceptions of improving the environment and life quality were higher, but reducing anger outbursts and resolving conflicts were lower. (2) Childhood living environments did not affect perceptions of social benefits, but current living environments did. Suburb residents understood reducing pain-relief medication demands and bearing larger-head babies better than city residents. City residents understood UGS’ investments considerable and sustained returns better than village residents. City residents agreed with accelerating patient recovery higher than village ones. (3) Respondents with “poor” self-rated health status had better perceptions of other social benefits. Those with “excellent” ratings did not fully understand UGS’ physical health benefits. “Poor” ratings understood improving a city’s image and making cities livable and sustainable better than “good” or “fair” ratings. “Excellent” ratings had less understanding of larger-head babies than “good” or “fair” ratings. The study could enhance appreciation of UGS’ social benefits to facilitate planning and management to meet residents’ expectations.
Associations of Central Sensitization-Related Symptoms with Low Back Pain-Related Factors and Work Status in Caregivers
Authors: Shigetoh Hayato , Abiko Teppei , Ohyama Michie , Sakata Eiji , Murata Shin
Published: August 2022

Low back pain (LBP) is associated with psychological factors and central sensitization-related symptoms (CSSs). The relationship between CSSs, LBP-related factors, and work status in caregivers remain unclear. This multicentre, collaborative, cross-sectional study aimed to determine the association between CSS severity, LBP-related factors, and work status in caregivers with LBP. We measured LBP intensity, pain duration, pain sites, CSSs (using the Central Sensitization Inventory-9: CSI-9), psychological factors (using the Pain Catastrophizing and Pain Self-Efficacy scales), and work status (interference, amount of assistance, frequency of assistance, and work environment) in 660 caregivers. CSS severity was categorised as no (CSI-9:0–9), mild (CSI-9:10–19), or moderate/severe (CSI-9:20–36). We further performed multiple comparison analyses and adjusted the residual chi-square to reveal differences between CSS severity groups. Caregivers with more severe CSSs tended to exhibit worse LBP intensity (p < 0.01), widespread pain (p < 0.01), catastrophic thinking (p < 0.01), and pain self-efficacy (p < 0.01), and they also tended to experience work interference (p < 0.01). Caregivers without CSSs tended to receive a smaller amount of assistance with a lower frequency (p < 0.05). The number of participants with an adequate environment and equipment was significantly less in the moderate/severe CSS group (p < 0.01). Thus, our findings may suggest that CSS severity is associated with LBP intensity, widespread pain, psychological factors, and work status in caregivers.
NDIS Participants with Psychosocial Disabilities and Life-Limiting Diagnoses: A Scoping Review
Authors: Boschen Kathy , Phelan Caroline , Lawn Sharon
Published: August 2022

This research aimed to map evidence about system supports and gaps for Australians with psychosocial disabilities and life-limiting diagnoses. A scoping review of available policy documents, academic, and grey literature was completed to discover key characteristics of this concept and provide context around the phenomenon. Our focus was on Australia’s National Disability Insurance Scheme (NDIS), a key reform providing support to the disability population nationally. No peer-reviewed or grey literature was retrieved on the phenomena. Therefore, three lines of enquiry were developed: experiences of NDIS participants living with psychosocial disabilities; the death, dying, and palliative care supports and experiences of NDIS participants of any disability type; and the experiences for people living with severe and persistent mental illness (SPMI) and life-limiting diagnoses. Five themes were identified: (1) the person; (2) advocacy; (3) informal supports; (4) formal supports; and (5) existing research. NDIS participants living with SPMI and their informal and formal support systems are still struggling to navigate the NDIS. While there are no specific publications about their end-of-life experiences, people with SPMI often experience poor end-of-life outcomes. Rigorous research into their death, dying, and palliative care experiences is needed to inform improved support to them, including their end-of-life care.
The Top 50 Most Cited Articles on Special Olympics: A Bibliometric Analysis
Authors: Khoo Selina , Ansari Payam , John Jacob , Brooke Mark
Published: August 2022

The Special Olympics was established in 1968 to “provide year-round sports training and athletic competition in a variety of Olympic-type sports for children and adults with intellectual disabilities”. It has gained recognition in the field of sports and healthcare of persons with intellectual disability, with a large number of dedicated researchers and institutions all over the world. However, there is an urgent need to analyze the progress and current status of this research field to identify knowledge gaps and develop this discipline. The aim of this study was to analyze the scientific production of the Special Olympics and report the bibliometric characteristics of the top 50 most cited Special Olympics publications. A systematic search was conducted on the Scopus database and bibliometric data were extracted and analyzed. The top 50 publications received 1632 citations. A total of 138 authors (63 female and 75 male) contributed to these publications. The two main areas of study were the physical health of Special Olympics athletes (n = 27) and the psycho-social health of athletes (n = 12).
Associations between Physical Fitness Index and Body Mass Index in Tibetan Children and Adolescents in Different High-Altitude Areas: Based on a Study in Tibet, China
Authors: Zhang Yunjie , Su Fan , Song Yongjing , Lu Jinkui
Published: August 2022

Objective: To investigate the relationship between physical fitness index (PFI) and body mass index (BMI) of Tibetan children and adolescents in different high-altitude areas in Tibet, China. Methods: Using the stratified cluster sampling method, 3819 Tibetan children and adolescents from three different high-altitude areas including Nyingchi, Lhasa and Nagqu in the Tibet area of China were given grip strength, standing long jump, sitting forward bend, 50 m running and endurance running tests. One-way analysis of variance was used to compare the physical fitness index in different high-altitude areas. In addition, the method of curve regression analysis was used to analyze the relationship between PFI and BMI. Results: In general, the level of PFI in Nagqu, Tibet, China was lower than that in Nyingchi and Lhasa, and the levels of girls were generally lower than those of boys. The proportions of malnourished, normal, overweight and obese Tibetan boys in high-altitude areas were 11.8%, 79.7%, and 8.5%; those of girls were 3.3%, 82.3%, and 14.4%, respectively. The curve regression analysis showed that the model fitting of male Nyingchi, Lhasa, Nagqu and female Nyingchi, Lhasa, Nagqu were all significant (F values were 29.697, 34.709, 37.500, 9.123, 9.785, 6.939, p < 0.01). The relationship between BMI and PFI generally showed an inverted “U” curve relationship. Conclusion: The negative impact of overweight and obesity on physical fitness of Tibetan boys in high-altitude areas is significantly higher than that of girls, and the negative impact of overweight and obesity on physical fitness of boys in Lhasa and Nyingchi area is more significant than that in the Nagqu area. In the future, attention should be paid to Lhasa and the occurrence of overweight and obesity among Tibetan boys in Nyingchi area in order to prevent the sharp decline of physical fitness and promote the physical and mental development of Tibetan children and adolescents in high-altitude areas.
Vertical Ramus Osteotomy, Is It Still a Valid Tool in Orthognathic Surgery?
Authors: Peleg Oren , Mahmoud Reema , Shuster Amir , Arbel Shimrit , Kleinman Shlomi , Mijiritsky Eitan , Ianculovici Clariel
Published: August 2022

The purpose of this study is to evaluate mandibular osteotomy procedures during orthognathic surgery, with an emphasis on the complications of the two leading procedures: intraoral vertical ramus osteotomy (IVRO) and sagittal split osteotomy (SSO). We conducted a retrospective cohort study by extracting the records of patients who underwent either IVRO or SSO procedures during orthognathic surgery in a single center between January 2010 and December 2019. A total of 144 patients were included (median age of 20.5 years, 52 males). The IVRO:SSO ratio was 118:26 procedures. When referring to all surgeries performed, IVRO procedures were associated with shorter hospitalization than the SSO procedures, while the overall durations of surgery and follow-up periods were comparable. In contrast, when referring only to bimaxillary procedures, the duration of the IVRO bimaxillary procedures was significantly shorter than the SSO bimaxillary procedures. There were 53 complications altogether. Postoperative complications consisting of skeletal relapse, temporomandibular joint dysfunction, sensory impairment, and surgical-site infection were significantly fewer in the IVRO group. Both types of osteotomies have acceptable rates of complications. IVRO appears to be a safer, simpler, though less acceptable procedure in terms of patient compliance.
TTN Variants Are Associated with Physical Performance and Provide Potential Markers for Sport-Related Phenotypes
Authors: Leońska-Duniec Agata , Borczyk Małgorzata , Piechota Marcin , Korostyński Michał , Brodkiewicz Andrzej , Cięszczyk Paweł
Published: August 2022

TTN encodes the third myofilament, titin, which plays structural, mechanical, regulatory, and developmental roles in sarcomeres. The aim of this research was to determine the interaction between novel and previously described TTN variants and athletic performance, as well as competition level, in Caucasians. Firstly, 100 athletes and 47 controls were recruited, and whole-genome sequencing was performed. Secondly, 348 athletes (108 endurance, 100 sprint/power, 140 mixed-sport athletes) and 403 volunteers were included, and real-time PCR was performed. We found a significant overrepresentation of the rs10497520 CT and TT genotypes in the sprint/power athlete group (95% CI, 1.41–3.66, p = 0.0013). The rs10497520 T carriers were 2.17 times more likely to become sprint/power athletes (95% CI 1.35–3.49, p = 0.0021). We also found that the likelihood of having the TT genotype was higher for the highly elite and sub-elite sprint/power athletes. Possessing at least one TAA (rs10497520, rs55837610, rs72648256) haplotype resulted in an increase in the log-odds ratio by 0.80 (p = 0.0015), 1.42 (p = 0.003), and 0.77 (p = 0.044) for all, highly elite, and sub-elite sprint/power athletes, respectively. We demonstrated that harbouring the rs10497520 T allele, individually and in a haplotype combination, increased the chance of being an elite sprint/power athlete, indicating that this allele may be favourable for sprint/power performance.
A Study on the Impact of Low-Carbon Technology Application in Agriculture on the Returns of Large-Scale Farmers
Authors: Huang Bingbing , Kong Hui , Yu Jinhong , Zhang Xiaoyou
Published: August 2022

The relationship and mechanism between agricultural low-carbon technology application and farm household returns are not yet clear, especially the lack of evidence from developing countries. This paper takes large-scale farming households in Jiangxi Province, China, from 2019 to 2020 as the research object, and obtains relevant data from field research to explore the intrinsic impact of agricultural low-carbon technology application on the returns of large-scale farming households. Based on the relevant theoretical analysis, the division dimensions of agricultural low-carbon technologies were proposed, and agricultural low-carbon technologies were subdivided into ten specific low-carbon technologies according to six dimensions: tillage system, breeding, fertilization, irrigation, medicine application, and waste treatment. Relevant questions were designed and researched to obtain data on the application status of low-carbon technologies in agriculture and the income cost status of large-scale farmers. Based on the theoretical analysis, the research hypotheses were proposed, and an empirical analysis was conducted based on the obtained data from large-scale farmers. The application of seven low-carbon technologies in agriculture: conservation tillage system, direct sowing technology, selection of compound fertilizer/organic fertilizer/controlled-release fertilizer, soil formula fertilization technology, deep fertilization/irrigation fertilization, sprinkler/drip irrigation/wet irrigation/intermittent irrigation, and straw resourceization significantly improved the income level of large-scale farmers. Furthermore, the application of biodegradable agricultural membranes, biopesticides, and new pesticide-controlled release technologies did not have significant effects on the income level of large-scale farmers, due to their low application and penetration rate. Based on the findings of the paper, the government should strengthen the promotion and subsidies of agricultural low-carbon technologies, especially those technologies that have no significant impact on large-scale farmers’ income, such as biodegradable agricultural membranes, biopesticides, and new pesticide controlled-release technologies, so as to achieve a win-win situation of reducing carbon emissions and increasing farmers’ income.
Evaluating Patients’ Experiences with Healthcare Services: Extracting Domain and Language-Specific Information from Free-Text Narratives
Authors: Jacennik Barbara , Zawadzka-Gosk Emilia , Moreira Joaquim Paulo , Glinkowski Wojciech Michał
Published: August 2022

Evaluating patients’ experience and satisfaction often calls for analyses of free-text data. Language and domain-specific information extraction can reduce costly manual preprocessing and enable the analysis of extensive collections of experience-based narratives. The research aims were to (1) elicit free-text narratives about experiences with health services of international students in Poland, (2) develop domain- and language-specific algorithms for the extraction of information relevant for the evaluation of quality and safety of health services, and (3) test the performance of information extraction algorithms’ on questions about the patients’ experiences with health services. The materials were free-text narratives about health clinic encounters produced by English-speaking foreigners recalling their experiences (n = 104) in healthcare facilities in Poland. A linguistic analysis of the text collection led to constructing a semantic–syntactic lexicon and a set of lexical-syntactic frames. These were further used to develop rule-based information extraction algorithms in the form of Python scripts. The extraction algorithms generated text classifications according to predefined queries. In addition, the narratives were classified by human readers. The algorithm-based and the human readers’ classifications were highly correlated and significant (p < 0.01), indicating an excellent performance of the automatic query algorithms. The study results demonstrate that domain-specific and language-specific information extraction from free-text narratives can be used as an efficient and low-cost method for evaluating patient experiences and satisfaction with health services and built into software solutions for the quality evaluation in health care.
Detection of Intestinal Dysbiosis in Post-COVID-19 Patients One to Eight Months after Acute Disease Resolution
Authors: Ferreira-Junior Alexandre Soares , Borgonovi Tais Fernanda , De Salis Larissa Vedovato Vilela , Leite Aline Zazeri , Dantas Amanda Soares , De Salis Guilherme Vedovato Vilela , Cruz Giuliano Netto Flores , De Oliveira Luiz Felipe Valter , Gomes Eleni , Penna Ana Lúcia Barretto , De Oliveira Gislane Lelis Vilela
Published: August 2022

The intestinal microbiota plays an important role in the immune response against viral infections, modulating both innate and adaptive immune responses. The cytokine storm is associated with COVID-19 severity, and the patient’s immune status is influenced by the intestinal microbiota in a gut-lung bidirectional interaction. In this study, we evaluate the intestinal microbiota of Brazilian patients in different post-COVID-19 periods, and correlate this with clinical data and the antibiotic therapy used during the acute phase. DNA extracted from stool samples was sequenced and total anti-SARS-CoV-2 antibodies and C-reactive protein were quantified. Compared with controls, there were significant differences in the microbiota diversity in post-COVID-19 patients, suggesting an intestinal dysbiosis even several months after acute disease resolution. Additionally, we detected some genera possibly associated with the post-COVID-19 dysbiosis, including Desulfovibrio, Haemophillus, Dialister, and Prevotella, in addition to decreased beneficial microbes, associated with antibiotic-induced dysbiosis, such as Bifidobacterium and Akkermansia. Therefore, our hypothesis is that dysbiosis and the indiscriminate use of antibiotics during the pandemic may be associated with post-COVID-19 clinical manifestations. In our study, 39% (n = 58) of patients reported symptoms, including fatigue, dyspnea, myalgia, alopecia, anxiety, memory loss, and depression. These data suggest that microbiota modulation may represent a target for recovery from acute COVID-19 and a therapeutic approach for post-COVID-19 sequelae.
Spatial Distribution, Potential Risks and Source Identification of Heavy Metals in the Coastal Sediments of the Northern Beibu Gulf, South China Sea
Authors: Yang Changping , Wang Liangming , Liu Yan , Shan Binbin , Sun Dianrong
Published: August 2022

Thirty samples of surface sediments (0–5 cm) from the northern Beibu Gulf were analyzed to determine the spatial distribution, potential risks and sources of six heavy metals (Cr, Cu, Zn, As, Cd and Pb). The concentrations (mg/kg, dw) of Cr, Cu, Zn, As, Cd and Pb were 15.38 ± 6.06, 6.54 ± 3.23, 41.86 ± 17.03, 6.92 ± 2.75, 0.04 ± 0.02 and 17.13 ± 6.38, respectively. Higher levels of Cr, Cu, Cd and Zn were observed in the western part of the study area. According to the potential ecological risk indexes and sediment quality guidelines, the measured metals were assessed at low contamination levels, with Pb posing the largest ecological risks. The results of positive matrix factorization (PMF) indicated that Cr and Zn mainly originated from natural geological background sources, while Cu, As, Cd and Pb were influenced by anthropogenic sources such as atmospheric deposition and anthropogenic activities. These three sources contributed 60.4%, 28.1% and 11.5% of the heavy metals, respectively. In addition, further research should be conducted focusing on the general relationships between As and various controls in sediments of the northern Beibu Gulf.
Evaluation of a Community-Led Program for Primordial and Primary Prevention of Rheumatic Fever in Remote Northern Australia
Authors: Ralph Anna P. , Kelly Angela , Lee Anne-Marie , Mungatopi Valerina L. , Babui Segora R. , Budhathoki Nanda Kaji , Wade Vicki , Dassel Jessica L. de , Wyber Rosemary
Published: August 2022

Environmental factors including household crowding and inadequate washing facilities underpin recurrent streptococcal infections in childhood that cause acute rheumatic fever (ARF) and subsequent rheumatic heart disease (RHD). No community-based ‘primordial’-level interventions to reduce streptococcal infection and ARF rates have been reported from Australia previously. We conducted a study at three Australian Aboriginal communities aiming to reduce infections including skin sores and sore throats, usually caused by Group A Streptococci, and ARF. Data were collected for primary care diagnoses consistent with likely or potential streptococcal infection, relating to ARF or RHD or related to environmental living conditions. Rates of these diagnoses during a one-year Baseline Phase were compared with a three-year Activity Phase. Participants were children or adults receiving penicillin prophylaxis for ARF. Aboriginal community members were trained and employed to share knowledge about ARF prevention, support reporting and repairs of faulty health-hardware including showers and provide healthcare navigation for families focusing on skin sores, sore throat and ARF. We hypothesized that infection-related diagnoses would increase through greater recognition, then decrease. We enrolled 29 participants and their families. Overall infection-related diagnosis rates increased from Baseline (mean rate per-person-year 1.69 [95% CI 1.10–2.28]) to Year One (2.12 [95% CI 1.17–3.07]) then decreased (Year Three: 0.72 [95% CI 0.29–1.15]) but this was not statistically significant (p = 0.064). Annual numbers of first-known ARF decreased, but numbers were small: there were six cases of first-known ARF during Baseline, then five, 1, 0 over the next three years respectively. There was a relationship between household occupancy and numbers (p = 0.018), but not rates (p = 0.447) of infections. This first Australian ARF primordial prevention study provides a feasible model with encouraging findings.
Survey and Measurements of Indoor Environmental Quality in Urban/Rural Schools Located in Romania
Authors: Catalina Tiberiu , Ghita Stefan Alexandru , Popescu Lelia Letiția , Popescu Răzvan
Published: August 2022

Taken as a whole, the indoor environmental quality (IEQ) is a concept that deals not only with thermal conditions, but it also goes much further, because it includes indoor air quality (IAQ), illuminance or acoustic comfort. Among the different categories of buildings, schools are one of the most important in society especially because future generations are spending more than 6 h/day and ensuring them a healthy and comfortable environment must be top priority. The purposes of this research were to better understand school children’s IEQ preferences and needs in classrooms and to compare these among rural versus urban respondents. To reach this goal, a simple yet complete survey was proposed and, along with measurements, multiple conclusions were made. The methodology proposed was to reach a large sample of respondents to give more credibility and precision to the analysis. The results are based on the responses of 790 occupants both from urban and rural environments. Lack of ventilation, cooling, low or too high temperatures during winter/summer periods and a misappropriate sizing or piloting of the heating system are some of the issues found during the survey. The paper tackles several issues at once, helping to paint a more holistic image of the problems encountered in school classrooms. Optimal thermal comfort is not met during the cold season in any of the schools under investigation. The old rural schools were found to be the worst performing buildings compared to urban schools where due to recent investments in refurbishment the winter thermal comfort was enhanced. During the study, it was observed that one of the major IAQ problems consisted in elevated levels of CO2 or particulate matter especially for the schools situated in the city. Noise was reported as an issue only for the schools situated next to national roads while visual comfort was found to be acceptable for more than 94% of respondents from all regions.
Determinants of Survival of HIV Patients Receiving Dolutegravir: A Prospective Cohort Study in Conflict-Affected Bunia, Democratic Republic of Congo
Authors: Buju Roger T. , Akilimali Pierre Z. , Tran Nguyen-Toan , Kamangu Erick N. , Mesia Gauthier K. , Kayembe Jean Marie N. , Situakibanza Hippolyte N.
Published: August 2022

This study aims to determine the factors influencing HIV-related mortality in settings experiencing continuous armed conflict atrocities. In such settings, people living with HIV (PLHIV), and the partners of those affected may encounter specific difficulties regarding adherence to antiretroviral therapy (ART), and retention in HIV prevention, treatment, and care programs. Between July 2019 and July 2021, we conducted an observational prospective cohort study of 468 PLHIV patients treated with Dolutegravir at all the ART facilities in Bunia. The probability of death being the primary outcome, as a function of time of inclusion in the cohort, was determined using Kaplan–Meier plots. We used the log-rank test to compare survival curves and Cox proportional hazard modeling to determine mortality predictors from the baseline to 31 July 2021 (endpoint). The total number of person-months (p-m) was 3435, with a death rate of 6.70 per 1000 p-m. Compared with the 35-year-old reference group, older patients had a higher mortality risk. ART-naïve participants at the time of enrollment had a higher mortality risk than those already using ART. Patients with a high baseline viral load (≥1000 copies/mL) had a higher mortality risk compared with the reference group (adjusted hazard ratio = 6.04; 95% CI: 1.78–20.43). One-fourth of deaths in the cohort were direct victims of armed conflict, with an estimated excess death of 35.6%. Improving baseline viral load monitoring, starting ART early in individuals with high baseline viral loads, the proper tailoring of ART regimens and optimizing long-term ART, and care to manage non-AIDS-related chronic complications are recommended actions to reduce mortality. Not least, fostering women’s inclusion, justice, peace, and security in conflict zones is critical in preventing premature deaths in the general population as well as among PLHIV.
Physical Activity, Energy Expenditure, Screen Time and Social Support in Spanish Adolescents—Towards an Explanatory Model about Health Risk Factors
Authors: Sanz-Martín Daniel , Melguizo-Ibáñez Eduardo , Ruiz-Tendero Germán , Zurita-Ortega Félix , Ubago-Jiménez José Luis
Published: August 2022

Youth obesity has been a pandemic for decades. One of its causes is a low level of physical activity. It is necessary to know the specific situation of adolescents and the factors that influence it in order to be able to act accordingly. The first aim of the current study is to create an explanatory model to establish the relationships between light physical activity time, light physical activity energy expenditure, screen time and social support. The second aim is to propose a theoretical model specifying the relationships between moderate–vigorous physical activity time, moderate–vigorous physical activity energy expenditure, screen time and social support. The study design was non-experimental (ex post facto), descriptive-correlational and cross-sectional. A total of 694 adolescents from the region of Soria (12–17 years) participated in the study. The instruments administered were the Four by One-Day Physical Activity Questionnaire, Parent Support Scale and Peer Support Scale. Two structural equation models were developed to analyse the relationships between the variables that comprised the explanatory models. The results show that social support had a negative influence on screen time in the proposed model in relation to light physical activity (r = −0.210; p ≤ 0.001) and in the proposed one regarding moderate–vigorous physical activity (r = −0.173; p ≤ 0.05). Social support was negatively related to light physical activity time (r = −0.167; p ≤ 0.05). Family support had a greater influence than did peer support. In conclusion, the models for light and moderate–vigorous physical activity are useful to describe the relationships between time, energy expenditure, screen time and social support.
Isolation and Optimisation of Culture Conditions for a Marine Bioflocculant-Producing Bacterium and Application of Its Bioflocculant in Wastewater Treatment
Authors: Selepe Tlou Nelson , Maliehe Tsolanku Sidney , Moganedi Kgabo , Masoko Peter , Mulaudzi Vusimuzi
Published: August 2022

The application of bioflocculants has become an alternative to that of chemical flocculants in wastewater treatment due to their environmental friendliness and non-toxic effects. This study aimed at isolating a bioflocculant-producing bacterium from marine water, optimisation of its culture conditions, and investigation of the removal efficiency of its bioflocculant on pollutants in wastewater. The bacterium was identified by 16S rRNA gene analysis. Optimal carbon and nitrogen sources, inoculum size, temperature, pH, and time were determined by the one-factor-at-a-time assay. The cytotoxicity of the bioflocculant was assessed on African green monkey kidney and bovine dermis cells using a tetrazolium-based columetric (MTT) method. Its removal efficiencies on chemical oxygen demand (COD), biological oxygen demand (BOD) and sulphur were determined using the Jar test method. The bacterial isolate was identified as Ochrobactrum oryzae AB84113. A maximum flocculating activity of 92% and a yield of 3.768 g/L were obtained when a 1% (v/v) inoculum size was used in the presence of starch and yeast extract at pH 7, 30 °C, and after 72 h of cultivation. The bioflocculant demonstrated non-cytotoxic effects on bovine dermis and African green monkey kidney cells. The bioflocculant removed 98% COD, 91% BOD and 86% of Sulphur. The bioflocculant has potential for pollutant removal from industrial wastewater.
Measuring COVID-19 Vulnerability for Northeast Brazilian Municipalities: Social, Economic, and Demographic Factors Based on Multiple Criteria and Spatial Analysis
Authors: Figueiredo Ciro José Jardim de , de Miranda Mota Caroline Maria , de Araújo Kaliane Gabriele Dias , Rosa Amanda Gadelha Ferreira , de Souza Arthur Pimentel Gomes
Published: August 2022

COVID-19 has brought several harmful consequences to the world from many perspectives, including social, economic, and well-being in addition to health issues. However, these harmful consequences vary in intensity in different regions. Identifying which cities are most vulnerable to COVID-19 and understanding which variables could be associated with the advance of registered cases is a challenge. Therefore, this study explores and builds a spatial decision model to identify the characteristics of the cities that are most vulnerable to COVID-19, taking into account social, economic, demographic, and territorial aspects. Hence, 18 features were separated into the four groups mentioned. We employed a model joining the dominance-based rough set approach to aggregate the features (multiple criteria) and spatial analysis (Moran index, and Getis and Ord) to obtain final results. The results show that the most vulnerable places have characteristics with high population density and poor economic conditions. In addition, we conducted subsequent analysis to validate the results. The case was developed in the northeast region of Brazil.
Kinetic Activity of Chromosomes and Expression of Recombination Genes in Achiasmatic Meiosis of Tityus (Archaeotityus) Scorpions
Authors: Almeida Bruno Rafael Ribeiro de , Noronha Renata Coelho Rodrigues , Cardoso Adauto Lima , Martins Cesar , Martins Jonas Gama , Procópio Rudi Emerson de Lima , Nagamachi Cleusa Yoshiko , Pieczarka Julio Cesar
Published: August 2022

Several species of Tityus (Scorpiones, Buthidae) present multi-chromosomal meiotic associations and failures in the synaptic process, originated from reciprocal translocations. Holocentric chromosomes and achiasmatic meiosis in males are present in all members of this genus. In the present study, we investigated synapse dynamics, transcriptional silencing by γH2AX, and meiotic microtubule association in bivalents and a quadrivalent of the scorpion Tityus maranhensis. Additionally, we performed RT-PCR to verify the expression of mismatch repair enzymes involved in crossing-over formation in Tityus silvestris gonads. The quadrivalent association in T. maranhensis showed delay in the synaptic process and long asynaptic regions during pachytene. In this species, γH2AX was recorded only at the chromosome ends during early stages of prophase I; in metaphase I, bivalents and quadrivalents of T. maranhensis exhibited binding to microtubules along their entire length, while in metaphase II/anaphase II transition, spindle fibers interacted only with telomeric regions. Regarding T. silvestris, genes involved in the recombination process were transcribed in ovaries, testes and embryos, without significant difference between these tissues. The expression of these genes during T. silvestris achiasmatic meiosis is discussed in the present study. The absence of meiotic inactivation by γH2AX and holo/telokinetic behavior of the chromosomes are important factors for the maintenance of the quadrivalent in T. maranhensis and the normal continuation of the meiotic cycle in this species.
Role of Autotaxin in High Glucose-Induced Human ARPE-19 Cells
Authors: Liu Yang , Yamagishi Reiko , Honjo Megumi , Kurano Makoto , Yatomi Yutaka , Igarashi Koji , Aihara Makoto
Published: August 2022

Autotaxin (ATX) is an enzymatic with lysophospholipase D (lysoPLD) activity. We investigated the role of ATX in high glucose (HG)-induced human retinal pigment epithelial (ARPE-19) cells to explore the pathogenesis of diabetic retinopathy (DR). We performed a quantitative real-time polymerase chain reaction, Western blotting, immunocytochemistry, enzyme-linked immunosorbent assay, cell permeability assay, and transepithelial electrical resistance measurement in HG-induced ARPE-19 cells and compared their results with those of normal glucose and osmotic pressure controls. ATX expression and its lysoPLD activity, barrier function, and expression of vascular endothelial growth factor receptors VEGFR-1 and VEGFR-2 were downregulated, while fibrotic responses, cytoskeletal reorganization, and transforming growth factor-β expression were upregulated, in the HG group. Our results suggest that HG induces intracellular ATX downregulation, barrier dysfunction, and fibrosis, which are involved in early DR and can be targeted for DR treatment.
Dlg Is Required for Short-Term Memory and Interacts with NMDAR in the Drosophila Brain
Authors: Bertin Francisca , Moya-Alvarado Guillermo , Quiroz-Manríquez Eduardo , Ibacache Andrés , Köhler-Solis Andrés , Oliva Carlos , Sierralta Jimena
Published: August 2022

The vertebrates’ scaffold proteins of the Dlg-MAGUK family are involved in the recruitment, clustering, and anchoring of glutamate receptors to the postsynaptic density, particularly the NMDA subtype glutamate-receptors (NRs), necessary for long-term memory and LTP. In Drosophila, the only gene of the subfamily generates two main products, dlgA, broadly expressed, and dlgS97, restricted to the nervous system. In the Drosophila brain, NRs are expressed in the adult brain and are involved in memory, however, the role of Dlg in these processes and its relationship with NRs has been scarcely explored. Here, we show that the dlg mutants display defects in short-term memory in the olfactory associative-learning paradigm. These defects are dependent on the presence of DlgS97 in the Mushroom Body (MB) synapses. Moreover, Dlg is immunoprecipitated with NRs in the adult brain. Dlg is also expressed in the larval neuromuscular junction (NMJ) pre and post-synaptically and is important for development and synaptic function, however, NR is absent in this synapse. Despite that, we found changes in the short-term plasticity paradigms in dlg mutant larval NMJ. Together our results show that larval NMJ and the adult brain relies on Dlg for short-term memory/plasticity, but the mechanisms differ in the two types of synapses.
Advanced Maternal Age Impairs Uterine Artery Adaptations to Pregnancy in Rats
Authors: Wooldridge Amy L. , Pasha Mazhar , Chitrakar Palehswan , Kirschenman Raven , Quon Anita , Spaans Floor , Sáez Tamara , Cooke Christy-Lynn M. , Davidge Sandra T.
Published: August 2022

Advanced maternal age (≥35 years) is associated with pregnancy complications. Aging impairs vascular reactivity and increases vascular stiffness. We hypothesized that uterine artery adaptations to pregnancy are impaired with advanced age. Uterine arteries of nonpregnant and pregnant (gestational day 20) young (4 months) and aged (9 months; ~35 years in humans) Sprague-Dawley rats were isolated. Functional (myogenic tone, n = 6–10/group) and mechanical (circumferential stress-strain, n = 10–24/group) properties were assessed using pressure myography and further assessment of elastin and collagen (histology, n = 4–6/group), and matrix metalloproteinase-2 (MMP-2, zymography, n = 6/group). Aged dams had worse pregnancy outcomes, including smaller litters and fetal weights (both p < 0.0001). Only in arteries of pregnant young dams did higher pressures (>100 mmHg) cause forced vasodilation. Across the whole pressure range (4–160 mmHg), myogenic behavior was enhanced in aged vs. young pregnant dams (p = 0.0010). Circumferential stress and strain increased with pregnancy in young and aged dams (p < 0.0001), but strain remained lower in aged vs. young dams (p < 0.05). Arteries from young nonpregnant rats had greater collagen:elastin ratios than the other groups (p < 0.05). In aged rats only, pregnancy increased MMP-2 active capacity. Altered functional and structural vascular adaptations to pregnancy may impair fetal growth and development with advanced maternal age.
Oxidised Low-Density Lipoprotein-Induced Platelet Hyperactivity—Receptors and Signalling Mechanisms
Authors: Berger Martin , Naseem Khalid M.
Published: August 2022

Dyslipidaemia leads to proatherogenic oxidative lipid stress that promotes vascular inflammation and thrombosis, the pathologies that underpin myocardial infarction, stroke, and deep vein thrombosis. These prothrombotic states are driven, at least in part, by platelet hyperactivity, and they are concurrent with the appearancxe of oxidatively modified low-density lipoproteins (LDL) in the circulation. Modified LDL are heterogenous in nature but, in a general sense, constitute a prototype circulating transporter for a plethora of oxidised lipid epitopes that act as danger-associated molecular patterns. It is well-established that oxidatively modified LDL promote platelet activation and arterial thrombosis through a number of constitutively expressed scavenger receptors, which transduce atherogenic lipid stress to a complex array of proactivatory signalling pathways in the platelets. Stimulation of these signalling events underlie the ability of modified LDL to induce platelet activation and blunt platelet inhibitory pathways, as well as promote platelet-mediated coagulation. Accumulating evidence from patients at risk of arterial thrombosis and experimental animal models of disease suggest that oxidised LDL represents a tangible link between the dyslipidaemic environment and increased platelet activation. The aim of this review is to summarise recent advances in our understanding of the pro-thrombotic signalling events induced in platelets by modified LDL ligation, describe the contribution of individual platelet scavenger receptors, and highlight potential future challenges of targeting these pathways.