bluuebunny/crossref_metadata_2025 huggingface dataset:
Synergy of Losartan and chemotherapy for patients with cholangiocarcinoma: A propensity score-matched analysis
Authors: Li Qing , Chang Zhenyu , Wang Tianyi , Liu Bing , Wang Ximin , Ge Xin-Yu , Yang Tao , Liu Qu , Wang Wei
Published: November 2022

Background

Cholangiocarcinoma (CCA) is a malignant tumor originating from bile duct epithelial cells that no obvious clinical symptoms and specific clinical manifestations are shown in the early stage of CCA.

Methods

Propensity score matching (PSM) is a quasi-experimental method in which this study used. Patients were enrolled from Department of General surgery, First Affiliated Hospital of Jinzhou Medical University from March 1, 2010, to December 30, 2019. Totally 170 patients with CCA were enrolled in this study.

Results

We performed a 1:2 PSM study and found that patients with losartan group showed both comparable median OS (overall survival) and TTR (time to recurrence) to those in the patients without losartan group before PSM. However, after matching, patients with losartan group showed favorable median OS and TTR than those in the patients without losartan group. Then we performed Cox proportional hazards models and found that patients with losartan was an independent factor after multivariable analysis for patients with CCA. Furtherly, we sequenced serial cfDNA were performed in 10 patients with losartan and 9 patients without losartan who received adjuvant chemotherapy after tumor resection. These results showed that the treatment of losartan was related with tumor microenvironment and could be potentially useful to combine the immunotherapy for patients with CCA.

Conclusion

In conclusion, this study demonstrated that the treatment of losartan could increase the efficacy of adjuvant chemotherapy and identified as an independent survival predictor for patients with CCA. Moreover, losartan could be potentially useful to combine the immunotherapy for patients with CCA.
The impact of the war on maintenance of long-term therapies in Ukraine
Authors: Khanyk Nataliia , Hromovyk Bohdan , Levytska Oksana , Agh Tamas , Wettermark Björn , Kardas Przemyslaw
Published: November 2022

Due to the Russian invasion, which started on 24 February 2022, the Ukrainian healthcare system is facing multiple challenges. A great number of healthcare facilities have been destroyed, while availability of other ones is often limited due to a lack of qualified medical staff. Certain services, e.g. cancer therapies, have been seriously disrupted. Moreover, millions of Ukrainians with chronic conditions are also suffering as due to war-related problems with execution of their long-term therapies. Availability of drugs is particularly limited in the occupied regions. According to the national statistics, as of 18 August 2022, about 505 pharmacies were damaged in Eastern Ukraine and 47 completely ruined. Moreover, the invaders have been blocking humanitarian aid provided to these territories by the Ukrainian government or other countries. Fortunately, in the areas controlled by the Government of Ukraine, the acute shortage of medicines, observed at the beginning of the war, has already been eliminated. Nevertheless, not all drugs are now fully available, even in the areas where no military attacks occur. The economic availability of drugs is also profoundly influenced by the significant increase in the cost of medications and the fall in average salaries. The Government of Ukraine is trying to minimise the impact of these war-related challenges by adopting a new legislation. This includes, among others, simplification of procedures for licensing, quality control and import of medicinal products to Ukraine. Other measures involve securing displaced people with the option of benefiting from local healthcare facilities, broadening the scope of the ePrescription system, authorizing primary care doctors to issue prescriptions to refugees, increasing the number of drugs reimbursed for long-term therapies, etc. These solutions, however, cannot balance all the harmful consequences the war in Ukraine brings in terms of maintenance of long-term therapies. Therefore, in order to minimise this negative impact, Ukraine still needs urgent international support in this area.
R-carvedilol, a potential new therapy for Alzheimer’s disease
Authors: Yao Jinjing , Chen S. R. Wayne
Published: November 2022

For decades, the amyloid cascade hypothesis has been the leading hypothesis in studying Alzheimer’s disease (AD) pathology and drug development. However, a growing body of evidence indicates that simply removing amyloid plaques may not significantly affect AD progression. Alternatively, it has been proposed that AD progression is driven by increased neuronal excitability. Consistent with this alternative hypothesis, recent studies showed that pharmacologically limiting ryanodine receptor 2 (RyR2) open time with the R-carvedilol enantiomer prevented and reversed neuronal hyperactivity, memory impairment, and neuron loss in AD mouse models without affecting the accumulation of ß-amyloid (Aβ). These data indicate that R-carvedilol could be a potential new therapy for AD.
Drug-related problems in patients admitted for SARS-CoV-2 infection during the COVID-19 pandemic
Authors: Barceló-Vidal J. , Echeverría-Esnal D. , Carballo N. , De Antonio-Cuscó M. , Fernández-Sala X. , Navarrete-Rouco M. E. , Colominas-González E. , Luque S. , Fuster-Esteva M. , Domingo L. , Sala M. , Duran X. , Grau S. , Ferrández O.
Published: November 2022

Introduction: Drug-related problems (DRP) are events or circumstances in which drug therapy does or could interfere with desired health outcomes. In December 2019, a new coronavirus, SARS-CoV-2, appeared. Little knowledge about this type of infection resulted in the administration of various drugs with limited use in other pathologies. Evidence about DRP in patients with COVID-19 is lacking.

Objective: The aim of the present study is to describe identified cases of DRP and those drugs involved in the first wave of patients with COVID-19, and evaluate associated risk factors.

Material and methods: Observational, retrospective study performed in a tertiary university hospital between 14th March 2020 and 31 May 2020 (corresponding to the first COVID-19 wave). We recruited patients admitted during the study period. Exclusion criteria included age < 18 years; admission to critically ill units; and care received either in the emergency room, at-home hospitalization or a healthcare center.

Results: A total of 817 patients were included. The mean age was 62.5 years (SD 16.4) (range 18–97), and 453 (55.4%) were male. A total of 516 DRP were detected. Among the patients, 271 (33.2%) presented at least one DRP. The mean DRP per patient with an identified case was 1.9. The prevailing DRPs among those observed were: incorrect dosage (over or underdosage) in 145 patients (28.2%); wrong drug combination in 131 (25.5%); prescriptions not in adherence to the then COVID-19 treatment protocol in 73 (14.1%); prescription errors due to the wrong use of the computerized physician order entry in 47 (9.2%); and incorrect dosage due to renal function in 36 (7%). The logistic regression analysis showed that patients who received only prescriptions of antibacterials for systemic use (J01 ATC group) faced a higher likelihood of experiencing a DRP (OR 2.408 (1.071–5.411), p = 0.033).

Conclusion: We identified several factors associated with an increased risk of DRPs, similar to those reported in other pre-pandemic studies, including a prolonged length of stay, higher number of prescribed drugs and antimicrobial administration. The relevance of pharmacists and tools like pharmacy warning systems can help prevent, identify and resolve DRP efficiently.
Effects of plyometric training on skill and physical performance in healthy tennis players: A systematic review and meta-analysis
Authors: Deng Nuannuan , Soh Kim Geok , Huang Dandan , Abdullah Borhannudin , Luo Shengyao , Rattanakoses Watnawat
Published: November 2022

Background: Plyometric training (PT) has been researched extensively in athletic populations. However, the effects of PT on tennis players are less clear.

Methods: We aim to consolidate the existing research on the effects of PT on healthy tennis players’ skill and physical performance. On 30th May 2022, a comprehensive search of SCOPUS, PubMed, Web of Science, and SPORTDiscus (via EBSCOhost) databases was performed. PICOS was employed to define the inclusion criteria: 1) healthy tennis players; 2) a PT program; 3) compared a plyometric intervention to a control group or another exercise group, and single-group trials; 4) tested at least one measures of tennis skill or physical performance; and 5) non-randomized study trials and randomized control designs. Individual studies’ methodological quality was evaluated by using the Cochrane RoB-2 and ROBINS-I instruments. Using Grading of Recommendations Assessment, Development, and Evaluation (GRADE), the certainty of the body of evidence for each outcome was assessed, and Comprehensive Meta-Analysis software was employed for the meta-analysis.

Results: Twelve studies comprising 443 tennis players aged 12.5–25 years were eligible for inclusion. The PT lasted from 3 to 9 weeks. Eight studies provided data to allow for the pooling of results in a meta-analysis. A moderate positive effect was detected for PT programs on maximal serve velocity (ES = 0.75; p < 0.0001). In terms of measures of physical performance, small to moderate (ES = 0.43–0.88; p = 0.046 to < 0.001) effects were noted for sprint speed, lower extremity muscle power, and agility. While no significant and small effect was noted for lower extremity muscle strength (ES = 0.30; p = 0.115). We found no definitive evidence that PT changed other parameters (i.e., serve accuracy, upper extremity power and strength, reaction time, and aerobic endurance). Based on GRADE, the certainty of evidence across the included studies varied from very low to moderate.

Conclusion: PT may improve maximal serve velocity and physical performance components (sprint speed, lower extremity muscular power, and agility) for healthy tennis players; however, more high-quality evidence about the effects of PT on the skill and physical performance of tennis players merits further investigation.

Systematic Review Registration: [https://inplasy.com/], identifier [INPLASY202250146].
Effects of Massa Medicata Fermentata on the intestinal pathogenic flagella bacteria and visceral hypersensitivity in rats with irritable bowel syndrome
Authors: Zhuang Zhaomeng , Huang Chen , Zhang Yiguang , Lv Bin
Published: November 2022

Objective: To investigate the effect of Massa Medicata Fermentata (MMF) on the changes of pathogenic flagellar bacteria and visceral hypersensitivity in rats with diarrhea irritable bowel syndrome (IBS-D).

Methods: Thirty adult SD rats were randomly divided into normal control group (n = 10), model control group (n = 10), and MMF group (n = 10). Acetic acid enema combined with restraint stress was used to build the IBS-D visceral hypersensitivity model; Abdominal withdrawal reflex (AWR) test was used to assess the visceral sensitivity of rats; 16SrRNA sequencing was used to analyze the changes of intestinal bacteria in each group, and the content of pathogenic flagellated bacteria were quantitatively counted; The content of flagellin in colonic mucosa was detected by ELISA; TLR5 protein in colonic mucosa of rats was detected by Western Blot.

Results: After IBS-D modeling, the visceral sensitivity of rats was significantly higher in the model control group than that in the normal control group (p = 0.0061), while it was significantly decreased in MMF group compared with the model control group (p = 0.0217), but without significant difference compared with the normal control group (p = 0.6851). The number of fecal Bifidobacterium and Lactobacillus in the model group were significantly decreased compared with the normal control group (p < 0.0001); While they were significantly increased in the MMF group compared with the model control group and normal control group (p = 0.009; p < 0.0001). The amount of fecal pathogenic flagellated bacteria in the model group was significantly increased compared with the normal control group (p = 0.001); However it was significantly reduced in MMF group compared with the model group (p = 0.026), which has no statistically difference with the normal control group (p = 0.6486). The content of flagellin in colonic mucosa was significantly increased in the model group when compared with the normal control group (p < 0.0001), and it was decreased in MMF group compared with the normal control group (p < 0.0001), but there was no statistical difference with the normal control group (p = 0.6545). The expression level of TLR5 protein in colonic mucosa of rat was significantly increased in model control group compared with the normal control group (p = 0.0034), However, it was significantly decreased in MMF group compared with normal control group (p = 0.0019), but it was no statistical difference with the normal control group (p = 0.7519).

Conclusion: MMF can reduce visceral hypersensitivity by decreasing the content of pathogenic flagellated bacteria and their flagellin and inhibiting its specific receptor TLR5 protein expression in colonic mucosa in IBS-D rats.
Facing energy limitations – approaches to increase basil (Ocimum basilicum L.) growth and quality by different increasing light intensities emitted by a broadband LED light spectrum (400-780 nm)
Authors: Tabbert Jenny Manuela , Riewe David , Schulz Hartwig , Krähmer Andrea
Published: November 2022

Based on the current trend towards broad-bandwidth LED light spectra for basil productions in multi-tiered controlled-environment horticulture, a recently developed white broad-bandwidth LED light spectrum (400-780 nm) including far-red wavelengths with elevated red and blue light fractions was employed to cultivate basil. Four Ocimum basilicum L. cultivars (cv. Anise, cv. Cinnamon, cv. Dark Opal and cv. Thai Magic) were exposed to two different rising light intensity conditions (ILow and IHigh). In dependence of the individual cultivar-specific plant height increase over time, basil cultivars were exposed to light intensities increasing from ~ 100 to ~ 200 µmol m-2 s-1 under ILow, and from 200 to 400 µmol m-2 s-1 under IHigh (due to the exponential light intensity increases with decreasing proximity to the LED light fixtures). Within the first experiment, basils’ morphological developments, biomass yields and time to marketability under both light conditions were investigated and the energy consumptions were determined to calculate the basils’ light use efficiencies. In detail, cultivar-dependent differences in plant height, leaf and branch pair developments over time are described. In comparison to the ILow light conditions, IHigh resulted in accelerated developments and greater yields of all basil cultivars and expedited their marketability by 3-5 days. However, exposure to light intensities above ~ 300 µmol m-2 s-1 induced light avoidance responses in the green-leafed basil cultivars cv. Anise, cv. Cinnamon and cv. Thai Magic. In contrast, ILow resulted in consumer-preferred visual qualities and greater biomass efficiencies of the green-leafed basil cultivars and are discussed as a result of their ability to adapt well to low light conditions. Contrarily to the green-leafed cultivars, purple-leafed cv. Dark Opal developed insufficiently under ILow, but remained light-tolerant under IHigh, which is related to its high anthocyanin contents. In a second experiment, cultivars’ volatile organic compound (VOC) contents and compositions over time were investigated. While VOC contents per gram of leaf dry matter gradually decreased in purple-leafed cv. Dark Opal between seedling stage to marketability, their contents gradually increased in the green cultivars. Regardless of the light treatment applied, cultivar-specific VOC compositions changed tremendously in a developmental stage-dependent manner.
A novel bacterial strain Burkholderia sp. F25 capable of degrading diffusible signal factor signal shows strong biocontrol potential
Authors: Yu Hongxiao , Chen Wen-Juan , Bhatt Kalpana , Zhou Zhe , Zhu Xixian , Liu Siqi , He Jiehua , Zhang Lian-Hui , Chen Shaohua , Wang Huishan , Liao Lisheng
Published: November 2022

Vast quantities of synthetic pesticides have been widely applied in various fields to kill plant pathogens, resulting in increased pathogen resistance and decreased effectiveness of such chemicals. In addition, the increased presence of pesticide residues affects living organisms and the environment largely on a global scale. To mitigate the impact of crop diseases more sustainably on plant health and productivity, there is a need for more safe and more eco-friendly strategies as compared to chemical prevention. Quorum sensing (QS) is an intercellular communication mechanism in a bacterial population, through which bacteria adjust their population density and behavior upon sensing the levels of signaling molecules in the environment. As an alternative, quorum quenching (QQ) is a promising new strategy for disease control, which interferes with QS by blocking intercellular communication between pathogenic bacteria to suppress the expression of disease-causing genes. Black rot caused by Xanthomonas campestris pv. campestris (Xcc) is associated with the diffusible signal factor (DSF). As detailed in this study, a new QQ strain F25, identified as Burkholderia sp., displayed a superior ability to completely degrade 2 mM of DSF within 72 h. The main intermediate product in the biodegradation of DSF was identified as n-decanoic acid, based on gas chromatography-mass spectrometry (GC-MS). A metabolic pathway for DSF by strain F25 is proposed, based on the chemical structure of DSF and its intermediates, demonstrating the possible degradation of DSF via oxidation-reduction. The application of strain F25 and its crude enzyme as biocontrol agents significantly attenuated black rot caused by Xcc, and inhibited tissue maceration in the host plant Raphanus sativus L., without affecting the host plant. This suggests that agents produced from strain F25 and its crude enzyme have promising applications in controlling infectious diseases caused by DSF-dependent bacterial pathogens. These findings are expected to provide a new therapeutic strategy for controlling QS-mediated plant diseases.
The effect of trust, IT knowledge, and entrepreneur’s innovativeness to embrace or shun the internet of things
Authors: Abushakra Ahmad , Nikbin Davoud , Odeh Ammar , Abdulwahab Rasha
Published: November 2022

This study examines critical factors influencing Omani entrepreneurs’ adoption of the internet of things (IoT) by expanding the constructs at the unified theory of acceptance and use of technology (UTAUT2) with entrepreneurs’ innovativeness, IT knowledge (ITK), and trust. A cross-sectional survey questionnaire was used to collect data from 158 entrepreneurs in Oman. Data were analyzed through the structural equation modeling technique using SmartPLS. The results indicated that performance expectancy, habit, social influence, trust (TR), ITK, and entrepreneurs’ innovativeness (PI) significantly affect Omani entrepreneurs’ intention to adopt IoT. Nonetheless, the results show that there is no significant relationship between hedonic motivation, effort expectancy, price value, and facilitating conditions to adopt IoT. This study contributes to previous literature by incorporating entrepreneurs’ innovativeness, ITK, and trust into UTAUT2. Furthermore, this study was conducted in a Middle Eastern country with solid support from the government for entrepreneurs; also, there is a gap in such studies in this area. This study helps practitioners in the field better understand how to influence entrepreneurs, push them toward using IoT applications further, and encourage non-users to start using them.
Visual over auditory superiority in sensorimotor timing under optimized condition
Authors: Zhan Liying , Huang Yingyu , Guo Zhihan , Yang Junkai , Gu Li , Zhong Shengqi , Wu Xiang
Published: November 2022

Auditory over visual advantage in temporal processing is generally appreciated, such as the well-established auditory superiority in sensorimotor timing. To test for a possible visual superiority in temporal processing, here, we present a data set composed of a large 60 subjects sample and a data set including eight smaller samples of approximately 15 subjects, showing that synchronization to a temporally regular sequence was more stable for a visual bouncing ball (VB) than for auditory tones (ATs). The results demonstrate that vision can be superior over audition in sensorimotor timing under optimized conditions, challenging the generally believed auditory superiority in temporal processing. In contrast to the auditory-specific biological substrates of timing in sensorimotor interaction, the present finding points to tight visual-motor cortical coupling in sensorimotor timing.
The relationship of emotional abuse, self-value and conflict resolution needs in secondary school students
Authors: Erses Tümen , Kiliç Yalin , Berkmen Bengü
Published: November 2022

The most crucial period for the teenagers is the secondary school period. The affection and knowledge that lead the student to build up his or her own self individuality, develop positive relationships, and cope with possible conflicts must be given to her or him by his or her family and neighborhood. The aim of this study was to analyze the correlation among the self-respect, the need for the solution of the conflict, social anxiety, and emotional exploitation within the range of the middle school students. The aforementioned research is such a kind of descriptive study which used one of the correlational survey research models, the quantitative method. In addition to the Personal Data Collection Document, the Scales of the Need of Conflict Resolution, emotional exploitation, the Rosenberg Self-Respect Scale, and the Social Anxiety Scale for the Teenagers are used. The research universe of this study is composed of 10.196 middle school students studying in the 6th, 7th, and the 8th year, at the public schools of the Ministry of Education of the TRNC. The sample group of the research is constituted by 530 volunteer middle school students from Famagusta, Guzelyurt, Lefke, Nicosia, and Kyrenia, respectively. The AMOS 2.1 and the SPSS 25 are used to resolve the data collected. The study that carried out through the structural equality method verifies that there is an agent role of the Need for the Conflict Resolution in between the self-respect and emotional exploitation. However, there cannot be verified any relation in between the self-respect and the social anxiety within the agent role of the Need for the Conflict Resolution. There has been verified meaningful and positive correlations among through the marks of the students taken from the NCR and the SAST, the NCR and the EES, the NCR and the RSRS, the SAST and the RSRS, the SAST and the EES, and the EES and the RSRS.
Improving gross motor skills of children through traditional games skills practiced along the contextual interference continuum
Authors: Hussain Bahar , Cheong Jadeera Phaik Geok
Published: November 2022

Gross motor skills (GMS) are the foundation for humans reaching an optimum level of motor competence necessary to undergo normal development, maintain health, and achieve athletic excellence. Yet, there is evidence that GMS levels of children are on a decline globally. Therefore, the main purpose of this study was to investigate the effectiveness of traditional cultural games (TCG) skills, practiced according to different amounts of contextual interference (CI), on the acquisition and retention of GMS. A total of 103 Pakistani primary school children aged between 7 and 10 years were recruited for this study. Participants were randomly assigned to four practice groups with different amounts of CI: Block (B) (low interference), gradually increasing (GI) (moderate interference), random (R) (high interference), and game-based (high interference). The Test of Gross Motor Development (TGMD-2) was used to assess four tasks [overhead throw (OT), underhand throw (UT), catch (C), and throwing to a target]. The test was administered on four occasions: during pre-test, post-test, retention, and transfer. The results showed that the R group outperformed all the other groups in the post-test and the retention test. Meanwhile, in the transfer test, both R and Game-Based groups performed better than the B and GI groups. There were no differences between the R and Game-Based groups during transfer. Practicing TCG skills according to a random order was better for the acquisition and learning of GMS. The CI effect was evident, whereby high interference practice schedules were superior to low and moderate interference practice schedules.
Effects of aerobics training on anxiety, depression and sleep quality in perimenopausal women
Authors: Zhao Yan , Niu Hualiang , Liu Shengjie
Published: November 2022

Objective

To investigate the effect of aerobics training on anxiety, depression and sleep disturbance in perimenopausal women.

Materials and methods

A total of 289 perimenopausal women with anxiety, depression or sleep disorder in Lishi district of Luliang city were treated with aerobics training. Self-rating anxiety scale, self-rating depression scale and Pittsburgh sleep scale were used to investigate the anxiety, depression and sleep status of perimenopausal women before and after intervention.

Results

After aerobics training, the scores of self-rating anxiety scale, self-rating depression scale and Pittsburgh sleep questionnaire were 48.26 ± 6.47, 50.27 ± 6.54 and 10.64 ± 4.38, respectively. The levels of anxiety, depression and sleep disorder in 289 subjects were significantly lower than those before intervention (t = 3.865, 4.541, 5.596, P < 0.01). The remission rate of symptoms was significantly different in subjects with different frequency of spontaneous practice (P < 0.01), and the higher the number of spontaneous practice, the higher the remission rate of anxiety, depression and sleep disorders in subjects (P < 0.01).

Conclusion

aerobics training can effectively improve the anxiety and depression of perimenopausal women, improve sleep quality, the more frequency of practice, the more obvious the effect. It can be used as an intervention to improve the mental health level of perimenopausal women in terms of anxiety, depression and sleep quality in clinical promotion.
Ultra-high field neuroimaging in psychosis: A narrative review
Authors: Lavigne Katie M. , Kanagasabai Kesavi , Palaniyappan Lena
Published: November 2022

Schizophrenia and related psychoses are complex neuropsychiatric diseases representing dysconnectivity across multiple scales, through the micro (cellular), meso (brain network), manifest (behavioral), and social (interpersonal) levels. In vivo human neuroimaging, particularly at ultra-high field (UHF), offers unprecedented opportunity to examine multiscale dysconnectivity in psychosis. In this review, we provide an overview of the literature to date on UHF in psychosis, focusing on microscale findings from magnetic resonance spectroscopy (MRS), mesoscale studies on structural and functional magnetic resonance imaging (fMRI), and multiscale studies assessing multiple neuroimaging modalities and relating UHF findings to behavior. We highlight key insights and considerations from multiscale and longitudinal studies and provide recommendations for future research on UHF neuroimaging in psychosis.
Relationship between negative coping style and fear of COVID-19 among Wuhan college students during the post-pandemic period: A moderated chain mediation model
Authors: Yang Lei , Yang Ziyun , Xia Ying
Published: November 2022

Objectives

After a long-term lockdown, particularly one in which human life is at risk, negative psychological consequences are expected. In this study, we aimed to explore the cause of stress and fear of coronavirus-19 (COVID-19) among Chinese college students in Wuhan during the latest strictest lockdown.

Methods

During the COVID-19 outbreak, 1,070 college students from Wuhan, aged 18–29 years, took part in an online survey. We used correlations, bootstrap tests, and other statistical analysis methods to analyze the data.

Results

Negative coping style significantly positively predicted fear of COVID-19, and stress had a significant mediating effect on the relationship between negative coping style and fear of COVID-19. In addition, rumination and stress had a chain-mediating effect on the relationship between negative coping style and fear of COVID-19. Perceived social support moderated the three paths of this serial mediation model.

Conclusion

Negative emotions, such as stress and fear of COVID-19, in college students are caused by both behavior (e.g., negative coping style) and cognition (e.g., rumination). Importantly, negative cognitive thinking (i.e., rumination) is often a malign consequence of a negative coping style. Thus, to improve students' mental health, students should be encouraged to engage in more positive behaviors and seek social support during periods of adherence to regular prevention and control measures.
Post-COVID-19 complications in home and hospital-based care: A study from Dhaka city, Bangladesh
Authors: Khandker Salamat , Akther Aivee , Syed Billal H. , Shafiullah Rezoun , Ahmed Kawsar , Chowdhury Alauddin A. , Khan Salim
Published: November 2022

A cross-sectional survey was undertaken to understand the management patterns and post-COVID-19 complications among hospital and home-treated participants. Retrospective information was collected from four COVID-19 dedicated hospitals and four selected community settings. Using probability proportional sampling, 925 participants were selected. Data were collected using a semi-structured questionnaire. Bivariate and multivariate logistic regression analysis and the exact chi-square tests were utilized to analyze the association between the studied variables. A total of 659 participants responded (response rate 70.93%); 375 from hospitals and 284 from communities. About 80% of participants were mild cases, 75% were treated at home, and 65% of hospital-treated participants were referred after home treatment. Participants treated at home-to hospital and directly in the hospital had 1.64 and 3.38 times longer recovery time respectively than what home-based participants had. A significant increasing trend (p < 0.001) of co-morbidities was found among referred and hospital treated participants. Age, level of education, physical exercise, practicing preventive measures, exposure to sunlight, and intake of carbohydrate, additional liquid, food supplements, and avoidance of junk foods were significantly associated with place of treatment. Post-COVID-19 difficulties of all factors were statistically significant for home treatment participants, whilst only depression (p = 0.026), chest pain (p = 0.017), and digestive disorders (p = 0.047) were significant (p < 0.05) for hospital treated participants. The outcomes from this study provide insight into a range of post-COVID-19 difficulties relating to at home and in hospital treatment participants. There are clear differences in the complications experienced, many of which are statistically significant. The health care professionals, the community people and COVID-19 survivors will be benefitted from the study findings, and the policy level people may use the information for designing health education program on post COVID-19 complications.
Network analysis of associations between anthropometry, physical fitness, and sport-specific performance in young canoe sprint athletes: The role of age and sex
Authors: Saal Christian , Chaabene Helmi , Helm Norman , Warnke Torsten , Prieske Olaf
Published: November 2022

Introduction

Anthropometric and physical fitness data can predict sport-specific performance (e.g., canoe sprint race time) in young athletes. Of note, inter-item correlations (i.e., multicollinearity) may exist between tests assessing similar physical qualities. However, multicollinearity among tests may change across age and/or sex due to age-/sex-specific non-linear development of test performances. Therefore, the present study aimed at analyzing inter-item correlations between anthropometric, physical fitness, and sport-specific performance data as a function of age and sex in young canoe sprint athletes.

Methods

Anthropometric, physical fitness, and sport-specific performance data of 618 male and 297 female young canoe sprint athletes (discipline: male/female kayak, male canoe) were recorded during a national talent identification program between 1992 and 2019. For each discipline, a correlation matrix (i.e., network analysis) was calculated for age category (U13, U14, U15, U16) and sex including anthropometrics (e.g., standing body height, body mass), physical fitness (e.g., cardiorespiratory endurance, muscle power), and sport-specific performance (i.e., 250 and 2,000-m on-water canoe sprint time). Network plots were used to explore the correlation patterns by visual inspection. Further, trimmed means (μtrimmed) of inter-item Pearson's correlations coefficients were calculated for each discipline, age category, and sex. Effects of age and sex were analyzed using one-way ANOVAs.

Results

Visual inspection revealed consistent associations among anthropometric measures across age categories, irrespective of sex. Further, associations between physical fitness and sport-specific performance were lower with increasing age, particularly in males. In this sense, statistically significant differences for μtrimmed were observed in male canoeists (p < 0.01, ξ = 0.36) and male kayakers (p < 0.01, ξ = 0.38) with lower μtrimmed in older compared with younger athletes (i.e., ≥U15). For female kayakers, no statistically significant effect of age on μtrimmed was observed (p = 0.34, ξ = 0.14).

Discussion

Our study revealed that inter-item correlation patterns (i.e., multicollinearity) of anthropometric, physical fitness, and sport-specific performance measures were lower in older (U15, U16) versus younger (U13, U14) male canoe sprint athletes but not in females. Thus, age and sex should be considered to identify predictors for sport-specific performance and design effective testing batteries for talent identification programs in canoe sprint athletes.
Clostridium autoethanogenum protein inclusion in the diet for broiler: Enhancement of growth performance, lipid metabolism, and gut microbiota
Authors: Wu Yushan , Wang Jing , Jia Ming , Huang Shixin , Cao Ying , Yao Ting , Li Junguo , Yang Yuxin , Gu Xu
Published: November 2022

This study aimed to investigate the effects of dietary supplementation of the new single-cell protein Clostridium autoethanogenum protein (CAP) on growth performance, plasma biochemical indexes, liver histology, lipid metabolism, and gut microbiota in Cobb broilers. According to the randomized block experimental design, 960 Cobb broilers (1d old) were divided into six treatments with eight replicates of 20 birds each. Six isonitrogenous and isoenergetic diets were formulated with different contents of CAP (0, 1, 2, 3, 4, and 5%) to replace soybean meal (SBM). The results showed that the addition of CAP did not influence liver health when it exceeded 2%. The protein metabolism markers and feed conversion rate increased (P < 0.05), significantly improving the growth performance. When the content of CAP was greater than 4%, it could promote lipolysis without affecting lipogenesis, decreasing the abdominal fat rate. There was no significant difference in MDA between these groups (P = 0.948). The increase of SOD and GSH-Px indicated the enhancement of antioxidant response. Alpha diversity did not significantly differ between groups (P > 0.05). Inclusion of 4% or less CAP led to the increase in beneficial microbiota, the concentration of short-chain fatty acids (SCFAs) such as acetic acid, propionic acid, and butyric acid (P < 0.05), and the concentration of primary bile acids such as cholic acid and goose deoxycholic acid (P < 0.05). While the concentration of secondary bile acids such as taurocholic acid and taurine goose deoxycholic acid was decreased (P < 0.05). These results illustrated that the CAP had a high potential for application in poultry nutrition. In terms of improving growth performance and antioxidant capacity and reducing fat deposition rate, 4% CAP content is recommended.
High energy diet of beef cows during gestation promoted growth performance of calves by improving placental nutrients transport
Authors: Kang Kun , Zeng Lei , Ma Jian , Shi Liyuan , Hu Rui , Zou Huawei , Peng Quanhui , Wang Lizhi , Xue Bai , Wang Zhisheng
Published: November 2022

The aim of this study was to explore the effects of dietary energy level during gestation on growth performance and serum parameters in offspring using beef cattle as research objects. Additionally, the gene expressions associated with nutrients transport in the placenta were evaluated. Eighteen Simmental crossbred cows (body weight = 338.44 ± 16.03 kg and 760 ± 6 days of age) were randomly assigned to 3 dietary treatment groups: low energy (LE, metabolic energy = 8.76 MJ/kg), medium (ME, 9.47 MJ/kg) and high (HE, 10.18 MJ/kg). The dietary treatments were introduced from day 45 before expected date of parturition. The pre-experiment lasted for 15 days and formal experiment lasted for 30 days. Growth performance data and blood samples of calves were collected at birth and day 30 post-birth. The placental tissue was collected at parturition. The results indicated that the birth weight and average daily gain of calves in HE group were higher (P < 0.05) than those in LE group. After parturition, the serum contents of glucose, total protein, cortisol and leptin in neonatal calves were significantly increased (P < 0.05) with the elevation of dietary energy levels. At 30 days postpartum, the glucose, glutathione peroxidase, growth hormone, insulin-like growth factor 1 and leptin concentrations of HE group were significantly increased (P < 0.05) as compared with LE group, while the serum amyloid protein A displayed an opposite trend between two groups. With the increase of dietary energy concentration, placental mRNA expressions of vascular endothelial growth factor A, glucose transporter 1 and 3 were significantly up-regulated (P < 0.05). Furthermore, the amino acid transporter solute carrier family 38 member 1, hydroxysteroid 11-beta dehydrogenase 2, insulin-like growth factor 1 and 2 mRNA expressions of HE group were higher (P < 0.05) than those of LE and ME groups. In conclusion, the improved growth performance of calves from the high energy ration supplemented beef cows may be attributed to the increased placental nutrients transport, which may lead to the increased nutrient supply to the fetus.
Temporal extracellular vesicle protein changes following intraarticular treatment with integrin α10β1-selected mesenchymal stem cells in equine osteoarthritis
Authors: Clarke Emily J. , Johnson Emily , Caamaño Gutierrez Eva , Andersen Camilla , Berg Lise C. , Jenkins Rosalind E. , Lindegaard Casper , Uvebrant Kristina , Lundgren-Åkerlund Evy , Turlo Agnieszka , James Victoria , Jacobsen Stine , Peffers Mandy J.
Published: November 2022

Introduction

Equine osteoarthritis (OA) is a heterogeneous, degenerative disease of the musculoskeletal system with multifactorial causation, characterized by a joint metabolic imbalance. Extracellular vesicles are nanoparticles involved in intracellular communication. Mesenchymal stem cell (MSC) therapy is a form of regenerative medicine that utilizes their properties to repair damaged tissues. Despite its wide use in veterinary practice, the exact mechanism of action of MSCs is not fully understood. The aim of this study was to determine the synovial fluid extracellular vesicle protein cargo following integrin α10β1-selected mesenchymal stem cell (integrin α10-MSC) treatment in an experimental model of equine osteoarthritis with longitudinal sampling.

Methods

Adipose tissue derived, integrin α10-MSCs were injected intraarticularly in six horses 18 days after experimental induction of OA. Synovial fluid samples were collected at day 0, 18, 21, 28, 35, and 70. Synovial fluid was processed and extracellular vesicles were isolated and characterized. Extracellular vesicle cargo was then analyzed using data independent acquisition mass spectrometry proteomics.

Results

A total of 442 proteins were identified across all samples, with 48 proteins differentially expressed (FDR ≤ 0.05) between sham-operated control joint without MSC treatment and OA joint treated with MSCs. The most significant pathways following functional enrichment analysis of the differentially abundant protein dataset were serine endopeptidase activity (p = 0.023), complement activation (classical pathway) (p = 0.023), and collagen containing extracellular matrix (p = 0.034). Due to the lack of an OA group without MSC treatment, findings cannot be directly correlated to only MSCs.

Discussion

To date this is the first study to quantify the global extracellular vesicle proteome in synovial fluid following MSC treatment of osteoarthritis. Changes in the proteome of the synovial fluid-derived EVs following MSC injection suggest EVs may play a role in mediating the effect of cell therapy through altered joint homeostasis. This is an important step toward understanding the potential therapeutic mechanisms of MSC therapy, ultimately enabling the improvement of therapeutic efficacy.
Hand Hygiene Social Norms Among Healthcare Workers During Early COVID-19: Results of a Global Survey
Authors: Gon Giorgia , Szekely Aron , Lowe Hattie , Tosi Marco
Published: November 2022

Objectives: Poor hand hygiene among healthcare workers is an important driver of infectious disease transmission. Although social norms are considered a key determinant of hand hygiene behaviour, little is known about them among healthcare workers. This study describes hand hygiene social norms among health workers, assesses their predictors, and tests if social expectations increased during the early stages of COVID-19.

Methods: We conducted a cross-sectional survey of healthcare workers from 77 countries (n = 1,233) from April to August 2020 assessing healthcare workers’ hand hygiene social expectations, personal normative beliefs, punishment and reward, and demographic factors. Linear regressions and hierarchical linear modelling were used to analyse the responses.

Results: We find high social expectations, personal beliefs, punishment, and rewards. Doctors tend to have lower social expectations than other occupation groups (e.g., nurses/midwives) and older respondents have higher social expectations. Social expectations increased during our survey, which may have been driven by COVID-19.

Conclusion: Our findings suggest that hand hygiene social norms are strong among healthcare workers with variation across occupation and age; their strength increased during the COVID-19 pandemic. These have implications for behaviour change in healthcare environments that could leverage more norm-targeting interventions.
The Use of the Polish Germplasm Collection of Nicotiana tabacum in Research and Tobacco Breeding for Disease Resistance
Authors: Czubacka Anna
Published: November 2022

The Polish germplasm collection of Nicotiana tabacum was started in the 1920s. Up to now, more than eight hundred accessions originating from different regions of the world have been gathered in the collection. It includes valuable breeding lines and obsolete cultivars, among them cytoplasmic male-sterile lines. Numerous cultivars are rich sources of features desired in tobacco breeding. Therefore, the accessions are continually characterised in terms of their various features, one of the most important of which is disease resistance. Much research is being done to explain the nature of resistance and its genetic basis. Moreover, cultivars with good agronomic characteristics are used in wide hybridisation, being recipients of resistance genes from wild species or are genetically modified with transgenes conditioning resistance. The biological diversity of cultivars also allows a proper selection of plant material for pathogen studies, while the large number of the accessions facilitates research into the conditions for long seed storage. Numerous examples of the use of Polish tobacco germplasm in research and breeding, specifically in disease resistance, have been presented in this paper.
Selection of High Yield and Stable Maize Hybrids in Mega-Environments of Java Island, Indonesia
Authors: Wicaksana Noladhi , Maulana Haris , Yuwariah Yuyun , Ismail Ade , Ruswandi Yasmin Anissa Robles , Ruswandi Dedi
Published: November 2022

Determination of grain yields of stable and high-yielding maize hybrids in a wide environment requires high accuracy. Many stability measurement methods have been used in multi-environment experiments. However, the relationships among the different methods are still difficult to understand. The objectives of this study were to 1. Identify the effect of growing season and location (Environments = E), hybrids (Genotypes = G), and their interactions (GEIs) on grain yields; 2. Select high-yielding and stable maize hybrids in a wide range of environments; 3. Determine the relationship between each stability estimation; and 4. Determine the mega-environment of maize hybrid and identify the best locations for testing. Field experiments were conducted at ten locations in Java Island, Indonesia, for two growing seasons using a randomized completed block design with three replications. The experimental results showed that the main effects of the growing season, location, hybrid, and GEIs, significantly affected maize hybrid yields. Stability estimations of TOP, S(3), S(6), NP(2), NP(3), KR, NP(4), CVi, and bi, belong to the concept of dynamic stability that can be used to select maize hybrids in favorable environments, while other estimations were classified as in the static stability. Three maize hybrids were successfully selected, with high and stable yields based on numerical and visual stability estimations, namely SC2, SC7, and SC9. The three hybrids can be used as candidates for sustainable maize development programs. The dry season, the rainy season, and the combination of two growing seasons produced three mega-environments. GJRS and KARS were the most discriminative environments. Both environments can be used as favorable environments for selecting the ideal maize hybrid.
Land Suitability for Cocoa Cultivation in Peru: AHP and MaxEnt Modeling in a GIS Environment
Authors: Rojas-Briceño Nilton B. , García Ligia , Cotrina-Sánchez Alexander , Goñas Malluri , Salas López Rolando , Silva López Jhonsy O. , Oliva-Cruz Manuel
Published: November 2022

Peru is one of the world’s leading exporters of cocoa beans, which directly impacts the household economy of millions of small farmers. Currently, the expansion and modernization of the cocoa-growing area require the zoning of the territory with suitable biophysical and infrastructural conditions to facilitate optimizing productivity factors. Therefore, we analyzed land suitability for cocoa (Theobroma cacao L.) production on the Peruvian mainland as a support measure for sustainable agriculture. To this end, the climatological, edaphological, orographic, and socioeconomic criteria determining sustainable cocoa cultivation were identified and mapped. Three modeling approaches (Analytic Hierarchy Process—AHP, Maximum Entropy—MaxEnt, and AHP—MaxEnt combined) were further used to hierarchize the importance of the criteria and to model the potential territory for sustainable cocoa cultivation. In all three modeling approaches, climatological criteria stood out among the five most important criteria. Elevation (orographic criteria) is also featured in this group. On the other hand, San Martin and Amazonas emerged as the five regions with the largest area ‘Highly suitable’ for cocoa cultivation in all three modeling approaches, followed by Loreto, Ucayali, Madre de Dios, Cusco, Junín, and Puno, which alternated according to modeling approach. From most to least restrictive, the AHP, MaxEnt, and AHP–MaxEnt modeling approaches indicate that 1.5%, 5.3%, and 23.0% of the Peruvian territory is ‘Highly suitable’ for cocoa cultivation, respectively.