bluuebunny/crossref_metadata_2025 huggingface dataset:
Outcomes at 7 Years of Age of Former Very Preterm Neonates with Repeated Surfactant Treatment for Prolonged Respiratory Distress in the Neonatal Period
Authors: Hascoet Jean-Michel , Deforge Hélène , Demoulin Silvia , Picaud Jean-Charles , Zupan Veronique , Ligi Isabelle , Moreau François , Labarre Aurelie , Daoud Patrick , Storme Laurent , Bonabel Claude , Hamon Isabelle
Published: September 2023

This study aimed at evaluating the 7-year outcomes of 118 very preterm newborns (VPNs, gestational age = 26 ± 1.4 w) involved in a randomized controlled trial. They presented neonatal respiratory distress (RDS), requiring ventilation for 14 ± 2 days post-natal age (PNA). A repeated instillation of 200 mg/kg poractant alfa (SURF) did not improve early bronchopulmonary dysplasia, but the SURF infants needed less re-hospitalization than the controls for respiratory problems at 1- and 2-year PNA. There was no growth difference at 7.1 ± 0.3 years between 41 SURF infants and 36 controls (80% of the eligible children), and 7.9% SURF infants vs. 28.6% controls presented asthma (p = 0.021). The children underwent cognitive assessment (WISC IV) and pulmonary function testing (PFT), measuring their spirometry, lung volume, and airway resistance. The spirometry measures showed differences (p < 0.05) between the SURF infants and the controls (mean ± standard deviation (median z-score)) for FEV1 (L/s) (1.188 ± 0.690(−0.803) vs. 1.080 ± 0.243 (−1.446)); FEV1 after betamimetics (1.244 ± 0.183(−0.525) vs. 1.091 ± 0.20(−1.342)); FVC (L) (1.402 ± 0.217 (−0.406) vs. 1.265 ± 0.267 (−1.141)), and FVC after betamimetics (1.452 ± 0.237 (−0.241) vs. 1.279 ± 0.264 (−1.020)). PFT showed no differences in the volumes or airway resistance. The global IQ median (interquartile range) was 89 (82:99) vs. 89 (76:98), with 61% of the children >85 in both groups. Repeated surfactant treatment in VPNs presenting severe RDS led to the attenuation of early lung injuries, with an impact on long-term pulmonary sequelae, without differences in neurodevelopmental outcomes.
Pre-Operative Ocular Findings and Long-Term Follow-Up in a Large Cohort of Non-Syndromic Unicoronal Craniosynostosis
Authors: Rostamzad Parinaz , Esser Yasmin S. , Tan Emily T. C. , Dremmen Marjolein H. G. , Pleumeekers Mieke M. , Loudon Sjoukje E.
Published: September 2023

(1) Background: Non-syndromic unicoronal craniosynostosis (UCS) is associated with a high prevalence of ocular anomalies. Currently, the etiology of this association remains obscure, however, it is presumed to be primarily attributed to their orbital malformations and/or secondary to craniofacial surgery. We assessed pre-operative ophthalmological examinations of non-syndromic UCS patients and compared them with their postoperative outcomes and long-term follow-up. (2) Methods: A retrospective case series was conducted on medical records of patients with non-syndromic UCS at Sophia Children’s Hospital, Rotterdam. Ophthalmologic examinations were collected at different time periods: T1 (first visit), T2 (<1 year after cranioplasty), and T3 (long-term follow-up at last visit). The McNemar’s test was used for statistical analysis. (3) Results: A total of 101 patients were included, for whom examinations were available at T1 and T3. Patients had a mean age of 2.8 years (±2.7) and 9.5 (±4.9) at T1 and T3, respectively. At T1, 52 patients (51.5%) were diagnosed with strabismus, and 61 patients (60.4%) at T3. Vertical strabismus increased significantly from 23 patients (22.8%) at T1 to 36 patients (35.6%) at T3 (p = 0.011). Followed by astigmatism, which increased significantly from 38 (37.6%) at T1 to 59 (58.4%) patients at T3 (p = 0.001). T1 was available in 20 patients prior to fronto-orbital advancement (FOA), therefore, a sub-analysis was conducted on these patients, which was followed shortly after FOA at T2. Prior to FOA, strabismus was present in 11 patients (55.0%) and in 12 patients (60.0%) at T2. After FOA, strabismus worsened in two patients. (4) Conclusions: This study showed the high prevalence of ocular anomalies in patients with non-syndromic UCS before and after cranioplasty and at long-term follow-up. The findings of this study show that ophthalmic and orthoptic examinations are an important part of the optimal treatment of patients with non-syndromic UCS.
Comparison of Several Anthropometric Indices Related to Body Fat in Predicting Cardiorespiratory Fitness in School-Aged Children—A Single-Center Cross-Sectional Study
Authors: Zadarko-Domaradzka Maria , Sobolewski Marek , Zadarko Emilian
Published: September 2023

Body fat (BF) and cardiorespiratory fitness (CRF) are important health markers that ought to be considered in screening exams. The aim of this study was to assess the value of six indicators, i.e., tri-ponderal mass index (TMI), relative fat mass (RFM), waist–BMI ratio, waist-to-height ratio (WHtR), waist-to-hip ratio (WHR) and body mass index (BMI) in predicting CRF in school-aged children. The analysis was based on the data coming from the examination of 190 children participating in school physical education (PE) classes. Their body weight (BW) and height (BH), waist and hip circumference (WC; HC) and percentage of body fat (%BF) were measured; the CRF test was performed with the use of the 20 m shuttle run test (20 mSRT); peak heart rate (HRpeak) was measured; TMI, relative fat mass pediatric (RFMp), waist–BMI ratio, WHtR, BMI and WHR were calculated. Statistical analysis was mainly conducted using regression models. The developed regression models, with respect to the sex and age of the children, revealed RFMp as the strongest CRF indicator (R2 = 51.1%) and WHR as well as waist–BMI ratio as the weakest ones (R2 = 39.2% and R2 = 40.5%, respectively). In predicting CRF in school-aged children, RFMp turned out to be comparable to body fat percentage obtained by means of the bioimpedance analysis (BIA) (R2 = 50.3%), and as such it can be used as a simple screening measure in prophylactic exams of school children. All of these models were statistically significant (p < 0.001).
The Validation of Two-Dimensional and Three-Dimensional Radiographic Measurements of Host Bone Coverage in Total Hip Arthroplasty for Hip Dysplasia: A Comparison with Intra-Operative Measurements
Authors: Yon Chang-Jin , Lee Kyung-Jae , Choi Byung-Chan , Suh Ho-Sung , Min Byung-Woo
Published: September 2023

Several methods have been introduced to measure the host bone coverage of the acetabular component after total hip arthroplasty (THA). The aims of this study were (1) to validate two-dimensional- and three-dimensional-based host bone coverage measurements by comparing intra-operative measurements, and (2) to determine the minimum host bone coverage for achieving stable cup fixation after THA in hip dysplasia. The clinical outcomes of each patient were evaluated during their final follow-up period using the Harris Hip score (HHS). The coverage of the host bone was analyzed by comparing 2D-based, 3D-based, and intraoperative assessments. The mean HHS was increased significantly from 60.84 ± 14.21 pre-operatively to 93.13 ± 4.59 (p < 0.0001). The host bone coverage ratio measured intraoperatively was 83.67 ± 3.40%, while the ratio measured by 3D CT reconstruction was 82.72 ± 3.59%. There was a strong positive correlation between the intra-operative host bone coverage and the 3D-based one (r = 0.826, p < 0.0001). It is recommended that 3D-based measurements are used to evaluate the host bone coverage after THA in patients with hip dysplasia. In addition, achieving a minimum host bone coverage of 75% is recommended for the attainment of stable cup fixation
Pseudocercospora fijiensis Conidial Germination Is Dominated by Pathogenicity Factors and Effectors
Authors: Carreón-Anguiano Karla Gisel , Gómez-Tah Rufino , Pech-Balan Efren , Ek-Hernández Gemaly Elisama , De los Santos-Briones César , Islas-Flores Ignacio , Canto-Canché Blondy
Published: September 2023

Conidia play a vital role in the survival and rapid spread of fungi. Many biological processes of conidia, such as adhesion, signal transduction, the regulation of oxidative stress, and autophagy, have been well studied. In contrast, the contribution of pathogenicity factors during the development of conidia in fungal phytopathogens has been poorly investigated. To date, few reports have centered on the pathogenicity functions of fungal phytopathogen conidia. Pseudocercospora fijiensis is a hemibiotrophic fungus and the causal agent of the black Sigatoka disease in bananas and plantains. Here, a conidial transcriptome of P. fijiensis was characterized computationally. Carbohydrates, amino acids, and lipid metabolisms presented the highest number of annotations in Gene Ontology. Common conidial functions were found, but interestingly, pathogenicity factors and effectors were also identified. Upon analysis of the resulting proteins against the Pathogen–Host Interaction (PHI) database, 754 hits were identified. WideEffHunter and EffHunter effector predictors identified 618 effectors, 265 of them were shared with the PHI database. A total of 1107 conidial functions devoted to pathogenesis were found after our analysis. Regarding the conidial effectorome, it was found to comprise 40 canonical and 578 non-canonical effectors. Effectorome characterization revealed that RXLR, LysM, and Y/F/WxC are the largest effector families in the P. fijiensis conidial effectorome. Gene Ontology classification suggests that they are involved in many biological processes and metabolisms, expanding our current knowledge of fungal effectors.
Effects of MAT1-2 Spore Ratios on Fruiting Body Formation and Degeneration in the Heterothallic Fungus Cordyceps militaris
Authors: Vu Tao Xuan , Thai Hanh-Dung , Dinh Bich-Hang Thi , Nguyen Huong Thi , Tran Huyen Thi Phuong , Bui Khanh-Linh Thi , Tran Tram Bao , Pham Hien Thanh , Mai Linh Thi Dam , Le Diep Hong , Nguyen Huy Quang , Tran Van-Tuan
Published: September 2023

The medicinal mushroom Cordyceps militaris is widely exploited in traditional medicine and nutraceuticals in Asian countries. However, fruiting body production in C. militaris is facing degeneration through cultivation batches, and the molecular mechanism of this phenomenon remains unclear. This study showed that fruiting body formation in three different C. militaris strains, namely G12, B12, and HQ1, severely declined after three successive culturing generations using the spore isolation method. PCR analyses revealed that these strains exist as heterokaryons and possess both the mating-type loci, MAT1-1 and MAT1-2. Further, monokaryotic isolates carrying MAT1-1 or MAT1-2 were successfully separated from the fruiting bodies of all three heterokaryotic strains. A spore combination of the MAT1-1 monokaryotic isolate and the MAT1-2 monokaryotic isolate promoted fruiting body formation, while the single monokaryotic isolates could not do that themselves. Notably, we found that changes in ratios of the MAT1-2 spores strongly influenced fruiting body formation in these strains. When the ratios of the MAT1-2 spores increased to more than 15 times compared to the MAT1-1 spores, the fruiting body formation decreased sharply. In contrast, when MAT1-1 spores were increased proportionally, fruiting body formation was only slightly reduced. Our study also proposes a new solution to mitigate the degeneration in the heterokaryotic C. militaris strains caused by successive culturing generations.
Crowdsourcing Intangible Heritage for Territorial Development: A Conceptual Framework Considering Italian Inner Areas
Authors: Tricarico Luca , Lorenzetti Edoardo , Morettini Lucio
Published: September 2023

This contribution aims to present a conceptual framework for developing territorial development strategies based on crowdsourcing technologies to enhance intangible heritage within the context of Italian inner areas. The work provides essential background information, examining technological aspects, defining intangible heritage precisely, and applying socially innovative strategies for marginal territories. Additionally, it offers a strategic framework to implement solutions that engage local communities and ensure widespread benefits. The study integrates methodologies of extensive literature review, policy analysis, and interactions with stakeholders during experimental fieldwork activities. Its objective is to bridge the gap between debates surrounding technological innovation, intangible heritage enhancement, and territorial development. The paper culminates in a synthesis of these aspects, offering a comprehensive information framework valuable for experts and scholars exploring these topics or undertaking projects aligned with these principles and tools.
Streamlining the Detection of Human Thyroid Receptor Ligand Interactions with XL1-Blue Cell-Free Protein Synthesis and Beta-Galactosidase Fusion Protein Biosensors
Authors: Hunt J. Porter , Free Tyler J. , Galiardi Jackelyn , Watt Kevin M. , Wood David W. , Bundy Bradley C.
Published: September 2023

Thyroid receptor signaling controls major physiological processes and disrupted signaling can cause severe disorders that negatively impact human life. Consequently, methods to detect thyroid receptor ligands are of great toxicologic and pharmacologic importance. Previously, we reported thyroid receptor ligand detection with cell-free protein synthesis of a chimeric fusion protein composed of the human thyroid receptor beta (hTRβ) receptor activator and a β-lactamase reporter. Here, we report a 60% reduction in sensing cost by reengineering the chimeric fusion protein biosensor to include a reporter system composed of either the full-length beta galactosidase (β-gal), the alpha fragment of β-gal (β-gal-α), or a split alpha fragment of the β-gal (split β-gal-α). These biosensor constructs are deployed using E. coli XL1-Blue cell extract to (1) avoid the β-gal background activity abundant in BL21 cell extract and (2) facilitate β-gal complementation reporter activity to detect human thyroid receptor ligands. These results constitute a promising platform for high throughput screening and potentially the portable detection of human thyroid receptor ligands.
Advancements in Additive Manufacturing of Tantalum via the Laser Powder Bed Fusion (PBF-LB/M): A Comprehensive Review
Authors: Mohsan Aziz Ul Hassan , Wei Dongbin
Published: September 2023

Additive manufacturing (AM) exhibits a prime increment in manufacturing technology development. The last few decades have witnessed massive improvement in this field of research, including the growth in the process, equipment, and materials. Irrespective of compelling technological advancements, technical challenges provoke the application and development of these technologies. Metal additive manufacturing is considered a prime sector of the industrial revolution. Various metal AM techniques, including Selective Laser Sintering (SLS), Laser Powder Bed Fusion (PBF-LB/M), and Electron Beam Powder Bed Fusion (PBF-EB/M), have been developed according to materials and process classifications. PBF-LB/M is considered one of the most suitable choices for metallic materials. PBF-LB/M of tantalum has become a hot topic of research in the current century owing to the high biocompatibility of tantalum and its high-end safety applications. PBF-LB/M of porous Ta can direct unexplored research prospects in biomedical and orthopedics by adapting mechanical and biomedical properties and pioneering implant designs with predictable features. This review primarily examines the current advancements in the additive manufacturing of tantalum and related alloys using the PBF-LB/M process. The analysis encompasses the evaluation of process parameters, mechanical properties, and potential biological applications. This will offer the reader valuable insights into the present state of PBF-LB/M for tantalum alloys.
Hot-Cracking Mechanism of Laser Welding of Aluminum Alloy 6061 in Lap Joint Configuration
Authors: Rakhi Km , Kang Seunggu , Shin Joonghan
Published: September 2023

Laser welding, known for its distinctive advantages, has become significantly valuable in the automotive industry. However, in this context, the frequent occurrence of hot cracking necessitates further investigation into this phenomenon. This research aims to understand the hot-cracking mechanism in aluminum alloy (AA) 6061, welded using a laser beam in a lap joint setup. We used an array of material characterization methods to study the effects of processing parameters on the cracking susceptibility and to elucidate the hot-cracking mechanism. A laser power of 2000 W generated large hot cracks crossing the whole weld zone for all welding speed conditions. Our findings suggest that using a heat input of 30 J/mm significantly mitigates the likelihood of hot cracking. Furthermore, we observed that the concentrations of the alloying elements in the cracked region markedly surpassed the tolerable limits of some elements (silicon: 2.3 times, chromium: 8.1 times, and iron: 2.7 times, on average) in AA6061. The hot-cracking mechanism shows that the crack initiates from the weld root at the interface between the two welded plates and then extends along the columnar dendrite growth direction. Once the crack reaches the central region of the fusion zone, it veers upward, following the cooling direction in this area. Our comprehensive investigation indicates that the onset and propagation of hot cracks are influenced by a combination of factors, such as stress, strain, and the concentration of alloying elements within the intergranular region.
Mass Spectrometry-Based Proteomics: Analyses Related to Drug-Resistance and Disease Biomarkers
Authors: Agostini Marco , Traldi Pietro , Hamdan Mahmoud
Published: September 2023

Mass spectrometry-based proteomics is a key player in research efforts to characterize aberrant epigenetic alterations, including histone post-translational modifications and DNA methylation. Data generated by this approach complements and enrich datasets generated by genomic, epigenetic and transcriptomics approaches. These combined datasets can provide much-needed information on various mechanisms responsible for drug resistance, the discovery and validation of potential biomarkers for different diseases, the identification of signaling pathways, and genes and enzymes to be targeted by future therapies. The increasing use of high-resolution, high-accuracy mass spectrometers combined with more refined protein labeling and enrichment procedures enhanced the role of this approach in the investigation of these epigenetic modifications. In this review, we discuss recent MS-based studies, which are contributing to current research efforts to understand certain mechanisms behind drug resistance to therapy. We also discuss how these MS-based analyses are contributing to biomarkers discovery and validation.
Pilot Study on the Molecular Pathogenesis of Pyeloureteral Junction Obstruction: Underdevelopment or Fibrosis?
Authors: Tamasauske Ramune Zilinskaite , Kazlauskas Vytis , Barasa Povilas , Krestnikova Natalija , Dasevicius Darius , Bilius Vytautas , Verkauskas Gilvydas
Published: September 2023

Background and Objectives: Congenital ureteral stenosis is one of the leading causes of impaired urinary drainage and subsequent dilatation of the urinary collecting system, known as hydronephrosis or ureterohydronephrosis. The mechanism that leads to obstruction is not clearly known. Multiple studies in rat models have shown increased angiotensin II and TGFβ levels in obstructed ureteral tissue. The aim of the study is to investigate the expression of fibrosis-related genes in obstructive and normal ureteral tissue. Material and Methods: It is a monocentric pilot study in which nineteen patients were selected prospectively. 17 patients underwent Hynes-Anderson pyeloplasty due to the PUJO; two patients underwent ureteroneocystostomy due to ureterovesical junction obstruction (UVJO); and six patients were chosen for the control group: five underwent nephrectomies due to the kidney tumor and one underwent upper pole heminephrectomy due to the duplex kidney with normal pyeloureteric junctions in all. Tissue RNA was chemically extracted after freezing the biopsy samples in liquid nitrogen, with cDNA synthesis performed immediately after nucleic acid isolation. qPCR was performed to evaluate the relative expression of Tgfb1, Mmp1, Timp1, Pai1, Ctgf, and Vegfa. Expression levels of the Gapdh and Gpi genes (geometric average) were used to calculate the relative expression of the investigated genes. Outliers were removed prior to calculating confidence intervals for the experimental groups, and a Wilcoxon rank-sum test was performed to determine the statistical significance of the differences. Results: Significant differences between healthy and stenotic tissue samples in Ctgf gene expression levels were observed, with the samples from afflicted tissue showing lower expression. No statistical difference in expression levels of Tgfb1, Timp1, Vegfa, Mmp1, and Pai1 was found. Conclusions: These findings suggest that tissue fibrosis, similar to other tissues and organs, is not the leading cause of stenosis, at least at the moment of surgery. Decreased CTGF expression is indicative of the developmental origin of obstruction.
Atomistic Simulations of Dislocation-Void Interactions in Concentrated Solid Solution Alloys
Authors: Vaid Aviral , Zaiser Michael , Bitzek Erik
Published: September 2023

This paper investigates the interaction of edge dislocations with voids in concentrated solid solution alloys (CSAs) using atomistic simulations. The simulation setup consists of edge dislocations with different periodicity lengths and a periodic array of voids as obstacles to dislocation motion. The critical resolved shear stress (CRSS) for dislocation motion is determined by static simulations bracketing the applied shear stress. The results show that shorter dislocation lengths and the presence of voids increase the CRSS for dislocation motion. The dislocation–void interaction is found to follow an Orowan-like mechanism, where partial dislocation arms mutually annihilate each other to overcome the void. Solute strengthening produces a ‘friction stress’ that adds to the Orowan stress. At variance with classical theories of solute pinning, this stress must be considered a function of the dislocation line length, in line with the idea that geometrical constraints synergetically enhance the pinning action of solutes. Modifying the equation by Bacon, Kocks and Scattergood for void strengthening to account for the solute hardening in CSAs allows one to quantitatively predict the CRSS in the presence of voids and its dependency on void spacing. The predictions show good agreement with the simulation data without invoking any fit parameters.
Martensite Decomposition and Ultrafine Grain Formation during Small Punch Creep Testing of Additively Manufactured Ti64
Authors: Lalé Mathieu , Malek Benaissa , Viguier Bernard
Published: September 2023

The creep behaviour of as-built additive-manufactured Ti-6Al-4V alloy was studied through small punch creep test (SPCT) experiments at 450 and 500 °C. The couple stress/minimum strain rate deduced from these tests made it possible to draw a Norton plot showing good agreement with tensile test creep results. The microstructure characterisation within the SPCT specimen evidenced the effect of local strain on microstructure evolution. After interrupted creep at 450 °C, in most deformed areas, the as-built martensite structure was fully decomposed to the α + β equilibrium phases, giving rise to a submicron equiaxed grain structure.
Performance and Quality Comparison of Movie Alignment Software for Cryogenic Electron Microscopy
Authors: Střelák David , Marchán Daniel , Carazo José María , S. Sorzano Carlos O.
Published: September 2023

Cryogenic electron microscopy (Cryo-EM) has been established as one of the key players in structural biology. It can reconstruct a 3D model of a sample at a near-atomic resolution. With the increasing number of facilities, faster microscopes, and new imaging techniques, there is a growing demand for algorithms and programs able to process the so-called movie data produced by the microscopes in real time while preserving a high resolution and maximal information. In this article, we conduct a comparative analysis of the quality and performance of the most commonly used software for movie alignment. More precisely, we compare the most recent versions of FlexAlign (Xmipp v3.23.03), MotionCor2 (v1.6.4), Relion MotionCor (v4.0-beta), Warp (v1.0.9), and CryoSPARC (v4.0.3). We tested the quality of the alignment using generated phantom data, as well as real datasets, comparing the alignment precision, power spectra density, and performance scaling of each program.
Fundamental Considerations and Analysis of the Energy Distribution in Laser Turning with Ultrashort Laser Pulses
Authors: Zettl Julian , Esen Cemal , Hellmann Ralf
Published: September 2023

This article discusses the process of the laser turning of rotational symmetric, cylindrical components using ultrashort laser pulses with respect to the geometrical conditions and the resulting energy distribution during the laser turning process. As a result, process predictions and potential process optimizations are feasible. Particular attention is drawn to the laser spot formation on the cylindrical surface of the work piece in conjunction with the positioning of the laser beam relative to the rotation axis of the specimen. Based on fundamental calculations and experimental results, an optimum processing strategy is discussed, whereat the use of a trepanning optic in the laser turning process and the forming of a particular surface structure is additionally being issued.
A Conformal Tri-Band Antenna for Flexible Devices and Body-Centric Wireless Communications
Authors: Awan Wahaj Abbas , Abbas Anees , Naqvi Syeda Iffat , Elkamchouchi Dalia H. , Aslam Muhammad , Hussain Niamat
Published: September 2023

A conformal tri-band antenna tailored for flexible devices and body-centric wireless communications operating at the key frequency bands is proposed. The antenna is printed on a thin Rogers RT 5880 substrate, merely 0.254 mm thick, with an overall geometrical dimension of 15 × 20 × 0.254 mm3. This inventive design features a truncated corner monopole accompanied by branched stubs fed by a coplanar waveguide. The stubs, varying in length, serve as quarter-wavelength monopoles, facilitating multi-band functionality at 2.45, 3.5, and 5.8 GHz. Given the antenna’s intended applications in flexible devices and body-centric networks, the conformability of the proposed design is investigated. Furthermore, an in-depth analysis of the Specific Absorption Rate (SAR) is conducted using a four-layered human tissue model. Notably, the SAR values for the proposed geometry at 2.45, 3.5, and 5.8 GHz stand at 1.48, 1.26, and 1.1 W/kg for 1 g of tissue, and 1.52, 1.41, and 0.62 W/kg for 10 g of tissue, respectively. Remarkably, these values comfortably adhere to both FCC and European Union standards, as they remain substantially beneath the threshold values of 1.6 W/kg and 2 W/kg for 1 g and 10 g tissues, respectively. The radiation characteristics and performance of the antenna in flat and different bending configurations validate the suitability of the antenna for flexible devices and body-centric wireless communications.
The Halotolerant Probiotic Bacterium Enterococcus lactis ASF-2 from Al-Asfar Lake, Saudi Arabia, Reduces Inflammation in Carrageenan-Induced Paw Edema
Authors: Alsaud Najla , Almajed Amjad , Lwusaybie Allujayn , Alsubaie Aljawharah , Alobaidan Hela , Alessa Jihad , Almousa Abeer , Ibrahim Hairul Islam M. , Khalifa Ashraf
Published: September 2023

Inflammation-related diseases are major causes of mortality and disability worldwide. This study aimed to identify and investigate probiotic bacteria that could be present in Al-Asfar Lake in Al-Ahsa City, Saudi Arabia to prevent the inflammatory responses of carrageenan-induced paw edema. In total, seven active strains were isolated, and three isolates (ASF-1, ASF-2, and ASF-3) exhibited a positive Gram stain and viable growth at 20% NaCl salinity; they also lacked catalase and hemolytic activities and had high levels of cell surface hydrophobicity (CSH). They also demonstrated potent antibacterial activity against Salmonella typhi and Staphylococcus aureus. These results revealed that ASF-2 had probiotic qualities, and it was selected for further research. ASF-2 demonstrated significant anti-inflammatory effects in an experimental model of carrageenan-induced paw edema; the experimental model showed decreased levels of pro-inflammatory markers, such as interleukin 6 (IL-6), interleukin 17 (IL-17), and transforming growth factor-β (TGF-β), and an increased level of an anti-inflammatory marker (interferon gamma (IFN-γ)). Animals in the control group saw a 45% decrease in edema when compared to mice in the carrageenan group. When comparing tissue damage and infiltration in the ASF-2-treated and non-treated mice, the histological examination of the sub-planar tissues of the hind leg revealed that the inflamed tissues had healed. The 16S rRNA sequencing method was utilized to establish that ASF-2 is, in fact, Enterococcus lactis with a 99.2% sequence similarity. These findings shed further light on ASF-2’s potential as a biocompatible anti-inflammatory medication.
Electrochemical Impedance as an Assessment Tool for the Investigation of the Physical and Mechanical Properties of Graphene-Based Cementitious Nanocomposites
Authors: Tziviloglou Eirini , Metaxa Zoi S. , Maistros George , Kourkoulis Stavros K. , Karousos Dionysios S. , Favvas Evangelos P. , Alexopoulos Nikolaos D.
Published: September 2023

This investigation explores the potential of electrochemical impedance spectroscopy (EIS) in evaluating graphene-based cementitious nanocomposites, focusing on their physical and structural properties, i.e., electrical resistivity, porosity, and fracture toughness. EIS was employed to study cement mixtures with varying graphene nanoplatelet (xGnP) concentrations (0.05–0.40% per dry cement weight), whereas flexural tests assessed fracture toughness and porosimetry analyses investigated the structural characteristics. The research demonstrated that the electrical resistivity initially decreased with increasing xGnP content, leveling off at higher concentrations. The inclusion of xGnPs correlated with an increase in the total porosity of the cement mixtures, which was indicated by both EIS and porosimetry measurements. Finally, a linear correlation emerged between fracture toughness and electrical resistivity, contributing also to underscore the use of EIS as a potent non-destructive tool for evaluating the physical and mechanical properties of conductive nano-reinforced cementitious nanocomposites.
Ionizing Radiation Sensing with Functionalized and Copper-Coated SWCNT/PMMA Thin Film Nanocomposites
Authors: Suman Guddi , Pulikkathara Merlyn , Wilkins Richard , Treadwell LaRico J.
Published: September 2023

This paper studies the ionizing radiation effects on functionalized single-walled carbon nanotube (SWCNT)/poly(methyl methacrylate) (PMMA) thin-film nanocomposites [SWNT/PMMA]. The functionalized thin-film devices are made of ferrocene-doped SWCNTs, SWCNTs functionalized with carboxylic acid (COOH), and SWCNTs coated/ modified with copper. The nanocomposite was synthesized by the solution blending method and the resulting nanocomposite was spin-cast on interdigitated electrodes (IDEs). A 160 kV X-ray source was used to irradiate the thin film and changes in the electrical resistance of the nanocomposites due to X-rays were measured using a semiconductor device analyzer. Carboxylic acid functionalized and copper-coated SWCNT/PMMA nanocomposite showed a reduced response to X-rays compared to unfunctionalized SWCNT/PMMA nanocomposite. Ferrocene-doped SWCNT showed a higher sensitivity to X-rays at lower dose rates. This is in contrast to a previous study that showed that similar nanocomposites using functionalized multi-walled CNTs (MWCNTs) had demonstrated an improved response to X-rays ionizing radiation compared to unfunctionalized MWCNTs for all dose rates. Electrical measurements were also performed using the Arduino Nano microcontroller. The result showed that a relatively economical, lightweight-designed prototype radiation sensor based on SWCNT/PMMA thin-film devices could be produced by interfacing the devices with a modest microcontroller. This work also shows that by encapsulating the SWCNT/PMMA thin-film device in a plastic container, the effect of ambient humidity can be reduced and the device can still be used to detect X-ray radiation. This study further shows that the sensitivity of SWCNT to X-rays was dependent on both the functionalization of the SWCNT and the dose rate.
Impact of Culinary Medicine Course on Confidence and Competence in Diet and Lifestyle Counseling, Interprofessional Communication, and Health Behaviors and Advocacy
Authors: Brennan Britta Retzlaff , Beals Katherine A. , Burns Ryan D. , Chow Candace J. , Locke Amy B. , Petzold Margaret P. , Dvorak Theresa E.
Published: September 2023

Most physicians report inadequate training to provide diet and lifestyle counseling to patients despite its importance to chronic disease prevention and management. To fill the nutrition training gap, elective Culinary Medicine (CM) courses have emerged as an alternative to curriculum reform. We evaluated the impact of an interprofessional CM course for medical and health professional students who experienced the hands-on cooking component in person or a in mixed-mode format (in-person and via Zoom) at the University of Utah from 2019–2023 (n = 84). A factorial ANOVA assessed differences between educational environment and changes between pre- and post-course survey responses related to diet and lifestyle counseling, interprofessional communication, and health behaviors and advocacy. Qualitative comments from post-course surveys were analyzed on a thematic level. Students rated themselves as having greater confidence and competence in diet and lifestyle counseling (p < 0.05) and increased ability to prepare eight healthy meals (p < 0.05). Additionally, a Mann–Whitney two-sample rank-sum test was used to compare data from exit survey responses from medical students who took the CM course (n = 48) and did not take the CM course (n = 297). Medical students who took CM were significantly more likely to agree that they could counsel patients about nutrition (p < 0.05) and physical activity (p < 0.05). CM courses may improve students’ confidence to provide diet and lifestyle counseling.
“Zoom”ing to the Kitchen: A Novel Approach to Virtual Nutrition Education for Medical Trainees
Authors: Charles Justin A. , Wood Nathan I. , Neary Stephanie , Moreno Jorge O. , Scierka Lindsey , Brink Benjamin , Zhao Xiwen , Gielissen Katherine A.
Published: September 2023

While nutritional interventions are first-line therapy for many chronic diseases, most medical trainees receive minimal nutrition education, leaving them unprepared to address nutritional issues with patients. An interactive, single-session, virtual nutrition curriculum was taught online to 80 physician assistant (PA) students. Topics included plant-based nutrition, dietary history-taking and counseling, and culinary medicine. Students were surveyed before, immediately after, and four weeks after the curriculum to assess changes to nutrition-related knowledge, attitudes, confidence, and personal dietary behaviors. Seventy-three PA students (91%) completed the pre-survey, 76 (95%) completed the post-survey, and 42 (52.5%) completed the delayed post-survey. Knowledge scores increased immediately post-intervention (48.9% to 78.9%; p < 0.001) and persisted four weeks later (78.9% to 75.8%; p = 0.54). Post-intervention, students felt more confident in dietary history-taking (55% vs. 95%; p = 0.001) and nutrition counseling (53% vs. 84%; p = 0.003) and agreed that dietary changes alone could reverse type 2 diabetes (74% vs. 97%; p = 0.027) and coronary artery disease (66% vs. 92%; p = 0.039). Curricula using virtual teaching kitchens may be a scalable approach to nutrition education for medical trainees.
Differences in the Factor Structure of the Eating Attitude Test-26 (EAT-26) among Clinical vs. Non-Clinical Adolescent Israeli Females
Authors: Spivak-Lavi Zohar , Latzer Yael , Stein Daniel , Peleg Ora , Tzischinsky Orna
Published: September 2023

In recent years, the diagnostic definitions of eating disorders (EDs) have undergone dramatic changes. The Eating Attitudes Test-26 (EAT-26), which is considered an accepted instrument for community ED studies, has shown in its factorial structure to be inconsistent in different cultures and populations. The aim of the present study was to compare the factor structure of the EAT-26 among clinical and non-clinical populations. The clinical group included 207 female adolescents who were hospitalized with an ED (mean age 16.1). The non-clinical group included 155 female adolescents (mean age 16.1). Both groups completed the EAT-26. A series of factorial invariance models was conducted on the EAT-26. The results indicate that significant differences were found between the two groups regarding the original EAT-26 dimensions: dieting, bulimia and food preoccupation, and oral control. Additionally, the factorial structure of the EAT-26 was found to be significantly different in both groups compared to the original version. In the clinical group, the factorial structure of the EAT-26 consisted of four factors, whereas in the non-clinical sample, five factors were identified. Additionally, a 19-item version of the EAT-26 was found to be considerably more stable and well suited to capture ED symptoms in both groups, and a cutoff point of 22 (not 20) better differentiated clinical samples from non-clinical samples. The proposed shortening of the EAT from 40 to 26 and now to 19 items should be examined in future studies. That said, the shortened scale seems more suited for use among both clinical and non-clinical populations. These results reflect changes that have taken place in ED psychopathology over recent decades.
Effects of Nanosecond Pulsed Electric Field on Immune Checkpoint Receptors in Melanoma Cells
Authors: Sauer Natalia , Szlasa Wojciech , Szewczyk Anna , Novickij Vitalij , Saczko Jolanta , Baczyńska Dagmara , Daczewska Małgorzata , Kulbacka Julita
Published: September 2023

Checkpoint molecules such as PD-1, LAG-3, and TIM-3 are currently under extensive investigation for their roles in the attenuation of the immune response in cancer. Various methods have been applied to overcome the challenges in this field. This study investigated the effects of nanosecond pulsed electric field (nsPEF) treatment on the expression of immune checkpoint molecules in A375 and C32 melanoma cells. The researchers found that the nsPEF treatment was able to enhance membrane permeabilization and morphological changes in the cell membrane without being cytotoxic. We found that the effects of nsPEFs on melanoma included (1) the transport of vesicles from the inside to the outside of the cells, (2) cell contraction, and (3) the migration of lipids from inside the cells to their peripheries. The treatment increased the expression of PD-1 checkpoint receptors. Furthermore, we also observed potential co-localization or clustering of MHC class II and PD-1 molecules on the cell surface and the secretion of cytokines such as TNF-α and IL-6. These findings suggest that nsPEF treatment could be a viable approach to enhance the delivery of therapeutic agents to cancer cells and to modulate the tumor microenvironment to promote an antitumor immune response. Further studies are needed to explore the mechanisms underlying these effects and their impacts on the antitumor immune response, and to investigate the potential of nsPEF treatment in combination with immune checkpoint inhibitors to improve clinical outcomes for cancer patients.
Exploring Cholinergic Compounds for Peripheral Neuropathic Pain Management: A Comprehensive Scoping Review of Rodent Model Studies
Authors: Montigné Edouard , Balayssac David
Published: September 2023

Neuropathic pain affects about 7–8% of the population, and its management still poses challenges with unmet needs. Over the past decades, researchers have explored the cholinergic system (muscarinic and nicotinic acetylcholine receptors: mAChR and nAChR) and compounds targeting these receptors as potential analgesics for neuropathic pain management. This scoping review aims to provide an overview of studies on peripheral neuropathic pain (PNP) in rodent models, exploring compounds targeting cholinergic neurotransmission. The inclusion criteria were original articles on PNP in rodent models that explored the use of compounds directly targeting cholinergic neurotransmission and reported results of nociceptive behavioral assays. The literature search was performed in the PubMed and Web of Science databases (1 January 2000–22 April 2023). The selection process yielded 82 publications, encompassing 62 compounds. The most studied compounds were agonists of α4β2 nAChR and α7 nAChR, and antagonists of α9/α10 nAChR, along with those increasing acetylcholine and targeting mAChRs. Studies mainly reported antinociceptive effects in traumatic PNP models, and to a lesser extent, chemotherapy-induced neuropathy or diabetic models. These preclinical studies underscore the considerable potential of cholinergic compounds in the management of PNP, warranting the initiation of clinical trials.
Vitamin E and Silymarin Reduce Oxidative Tissue Damage during Gentamycin-Induced Nephrotoxicity
Authors: Georgiev Tsvetelin , Nikolova Galina , Dyakova Viktoriya , Karamalakova Yanka , Georgieva Ekaterina , Ananiev Julian , Ivanov Veselin , Hadzhibozheva Petya
Published: September 2023

Aminoglycoside antibiotics and gentamicin (GN), in particular, are still widely used in clinical practice. It is a well-known fact that GN causes nephrotoxicity, and redox disturbances are discussed as a factor in its side effects. Recently, a new type of cell oxidative death, named ferroptosis, was discovered; it is associated with iron accumulation in the cell, glutathione (GSH) depletion and inactivation of glutathione peroxidase-4 (GPX4), reactive oxygen species (ROS) increment with concomitant lipid peroxidation. In this regard, a possible connection between GN-induced renal damage, ferroptosis and the overall antioxidant status of the organism could be investigated. Moreover, due to its beneficial effects, GN is still one of the main choices as a therapeutic agent for several diseases, and the possible reduction of its side effects with the application of certain antioxidants will be of important clinical significance. The study was conducted with adult male white mice divided into several groups (n = 6). GN nephrotoxicity was induced by the administration of GN 100–200 mg/kg i.p. for 10 days. The control group received only saline. The other groups received either Vitamin E (400 mg/kg p.o.) or Silymarin (200 mg/kg p.o.) applied alone or together with GN for the same period. After the end of the study, the animals were sacrificed, and blood and tissue samples were taken for the assessment of biochemical parameters and antioxidant status, as well as routine and specific for GPX4 histochemistry examination. The experimental results indicate that GN-induced nephrotoxicity negatively modulates GPX4 activity and is associated with increased production of ROS and lipid peroxidation. The groups treated with antioxidants demonstrated preserved antioxidant status and better GPX4 activity. In conclusion, the inhibition of ROS production and especially the suppression of ferroptosis, could be of clinical potential and can be applied as a means of reducing the toxic effects of GN application.
StartXFit—Nine Months of CrossFit® Intervention Enhance Cardiorespiratory Fitness and Well-Being in CrossFit Beginners
Authors: Schlie Jennifer , Brandt Tom , Schmidt Annette
Published: September 2023

Insufficient physical activity (PA) is associated with low cardiorespiratory fitness, which favors cardiovascular and other noncommunicable diseases. Additionally, it evidentially affects mental health. Considering the WHO PA guidelines, CrossFit® represents a versatile exercise program that combines aerobic and resistance training with mobility and could help reduce disease incidences among sedentary people. Yet, long-term CrossFit research is sparse. We conducted a nine-month intervention (≥2 CrossFit workouts/week) in 16 beginners (14 males, 35 ± 6.8 years, 180 ± 8.6 cm, 85. 5 ± 19.1 kg). As a primary endpoint, VO2max was assessed at baseline, four, and nine months. A repeated-measures ANOVA and Pearson correlation were conducted. Well-being was investigated by the WHO-5 Index pre- and post-intervention. For exploratory purposes, body composition and heart rate recovery (HRR) were tracked. In a second step, all males were categorized into two groups based on body fat percentage and analyzed by repeated measures ANOVA again. The main outcome was an 11.5% VO2max improvement with a large effect (p < 0.01, ηp2 = 0.27). Strong negative correlations between baseline VO2max and its progression after nine months (p = 0.006, r = −0.654) were found. Well-being increased by 8.7% (p = 0.024, d = 0.51). HRR improved both at 1 min (p < 0.05, ηp2 = 0.34) and at 5 min (p < 0.05, ηp2 = 0.27) post-exercise. Resting metabolic rate increased by 2.2% (p = 0.042). Analysis by group revealed improved HRR at 1 min (p < 0.05, ηp2 = 0.62) only for the “high body fat” group. This study reveals the potential of CrossFit to enhance physiological and psychological health in beginners. For more robust results, larger sample sizes with a higher proportion of women are needed.
Mutagenesis in the Age of Next-Generation-Sequencing and Genome Editing
Authors: Xin Zhanguo
Published: September 2023

Mutagenesis is a proven, classical technique for inducing a broad spectrum of DNA variations and has led to the creation of thousands of improved varieties in many crop species [...]
Regional Pollination Activity by Moth Migration in Athetis lepigone
Authors: Jia Huiru , Chen Yuchao , Li Xiaokang , Pan Yunfei , Liu Dazhong , Liu Yongqiang , Wu Kongming
Published: September 2023

Nocturnal moths (Lepidoptera) are important pollinators of a wide range of plant species. Understanding the foraging preferences of these insects is essential for their scientific management. However, this information is lacking for most moth species. The present study was therefore conducted to delineate the host plant feeding and pollination ranges of an agriculturally important nocturnal moth species Athetis lepigone by identifying the pollen species adhering to their bodies during long-distance migration. Pollen grains were dislodged from 1871 A. lepigone migrants captured on Beihuang Island in the Bohai Strait between 2020 and 2021. This region is a key seasonal migration pathway for A. lepigone in northern China. Almost 20% of all moths sampled harbored pollens, providing direct evidences that this moth species may serve as pollinators. Moreover, at least 39 pollen taxa spanning 21 plant families and 31 genera were identified, with a preference for Asteraceae, Amaranthaceae, and Pinaceae. Additionally, the pollen adherence ratios and taxa varied with moth sex, inter-annual changes, and seasonal fluctuations. Most importantly, the pollen taxa were correlated with insect migration stages and indicated that A. lepigone bidirectionally migrates between central China (Shandong, Hebei, and Henan Provinces) and northeastern China (Liaoning Province). Overall, the findings of the present work provide valuable information on the pollination behavior, geographical origins, and pollination regions of A. lepigone moths and could facilitate the design and optimization of efficacious local and regional management strategies for this important insect.