bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimization of Blades and Impellers for Electric Vehicle Centrifugal Pumps via Numerical Analysis
Authors: Jeon Hyeonchang , Hyun Daeil , Lee Hyuntae , Son Seongjin , Han Jaeyoung
Published: February 2024

Since the 2015 Paris Agreement, efforts for environmental protection have gained prominence worldwide. Accordingly, electric vehicles have become increasingly relevant. Thus, improving the performance of the water pump, a key component of cooling systems in electric vehicles, is crucial. Electric vehicles operate on batteries and motors, making their cooling systems remarkably complex. Efficient operation of the water pump is directly related to the stable performance of electric vehicles and is therefore critical. This study conducted numerical analyses using Ansys Fluent to evaluate water pump performance by varying key parameters, namely, number of blades and outer diameter of the impeller. When the number of blades was changed to 7, 9, 11, and 13, the efficiency, head, and thrust tended to increase. In particular, for blade counts greater than 11, the fluid flow was found to stabilize with negligible effect on pump performance. When the outer diameter of the impeller was 70, 69, 68, and 67 mm, although efficiency decreased, the head and thrust tended to increase. Based on these comprehensive results, a structure was proposed for the shape of the optimized water pump. The development of efficient and stable water pumps is expected to contribute to the performance improvement of electric vehicles.
The Nexus between Wholesale Electricity Prices and the Share of Electricity Production from Renewables: An Analysis with and without the Impact of Time of Distress
Authors: Herczeg Balázs , Pintér Éva
Published: February 2024

The continuous integration of renewable energy sources into the EU’s energy mix is reshaping the electricity market dynamics mainly due to the merit order mechanism affecting wholesale electricity prices (WHEP). This article aims to review the wholesale electricity market mechanism, identify the key factors affecting WHEP, and assess the extent of their contributions under different circumstances. Time series datasets, consisting of monthly observations of commodity prices and energy data regarding 25 EU members over the time horizons January 2015–December 2020 (pre-crisis) and January 2015–August 2023 (co-crisis), are used to support the theory, perform the comparison, and verify the validity of our hypotheses with the use of correlation and multiple linear regression analyses. Our empirical results show that in both cases, a 1% increase in the share of renewable electricity generation (RES) from one period to the next is ceteris paribus associated with an average of approx. 0.96% decrease in WHEP for the same period. However, extreme natural gas prices during times of distress significantly increase WHEP due to the merit order mechanism, from an average of 0.19% to 0.55%. This novel approach provides deeper insights into the interconnectedness of WHEP and the energy and environmental commodity prices and RES during changing economic and geopolitical circumstances, primarily highlighting the influencing factor of RES in WHEP developments.
Power System Decarbonization Assessment: A Case Study from Taiwan
Authors: Wang Chun-Kai , Lee Chien-Ming
Published: February 2024

The first global stocktake (GST) at 2023 UN Climate Change Conference (COP28) pointed out that accelerating the phasing down of fossil fuels has become an important mitigation policy to maintain a maximum temperature limit of 1.5 °C. The optimal power portfolio for achieving Taiwan’s net-zero emissions by 2050 is evaluated from the perspective of sustainable development. This study is enhances the 2021 research findings of Wang et al. on the sustainable power model, incorporating homogenized cost and technical constraints for empirical analysis. The results indicated that renewable energy sources play a pivotal role in achieving net-zero emissions. Gas power generation requires careful consideration, including early decommissioning or the adoption of carbon capture and storage (CCS) technology to prevent carbon lock-in and compete with hydrogen energy technology. Notably, coal combined with CCS technology offers a viable option for a cost-effective roadmap for a decarburized power generation portfolio by 2050, serving as a reference for national planning strategies for promoting net-zero emissions.
Concentrations of Potentially Toxic Elements in Topsoils of Urban Agricultural Areas of Rome
Authors: Chandramohan Monica Shree , da Silva Isabel Martinho , da Silva Joaquim Esteves
Published: February 2024

Urban agricultural soils have important social, environmental, and economic roles in big cities, contributing to their sustainability. However, food production in urban soils may be compromised due to soil pollution that resulted from decades of industrial, non-regulated environmental activity and mobile transport. In this study, 12 soils from the urban agricultural area of Rome (Italy) were analyzed for the potentially toxic elements (PTEs) Be, Ba, Pb, Co, Ni, V, Zn, Hg, Cd, As, Cu, and Cr. All but one of the soils under analysis were characterized by at least one PTE concentration above the threshold limit defined by the D.Lgs 152/06 for agricultural soils. Multivariate analysis showed that the soils could be classified into five clusters: clusters I and II had relatively lower mean PTE concentrations; clusters III, IV, and V had relatively higher mean PTE concentrations with several PTE concentrations above the threshold proposed by ILD. Three factors contributing to the variability of the PTE’s concentration in the soils under investigation were identified: a geological factor related to PTE As, Ba, Be, and V; an anthropogenic factor related to Pb and Cu; and a mixed factor related to Co, Cr, Ni, and Zn. High PTE content may limit the utilization of urban soils for food production.
A Comparative Analysis of Mouse Imprinted and Random X-Chromosome Inactivation
Authors: Malcore Rebecca M. , Kalantry Sundeep
Published: February 2024

The mammalian sexes are distinguished by the X and Y chromosomes. Whereas males harbor one X and one Y chromosome, females harbor two X chromosomes. To equalize X-linked gene expression between the sexes, therian mammals have evolved X-chromosome inactivation as a dosage compensation mechanism. During X-inactivation, most genes on one of the two X chromosomes in females are transcriptionally silenced, thus equalizing X-linked gene expression between the sexes. Two forms of X-inactivation characterize eutherian mammals, imprinted and random. Imprinted X-inactivation is defined by the exclusive inactivation of the paternal X chromosome in all cells, whereas random X-inactivation results in the silencing of genes on either the paternal or maternal X chromosome in individual cells. Both forms of X-inactivation have been studied intensively in the mouse model system, which undergoes both imprinted and random X-inactivation early in embryonic development. Stable imprinted and random X-inactivation requires the induction of the Xist long non-coding RNA. Following its induction, Xist RNA recruits proteins and complexes that silence genes on the inactive-X. In this review, we present a current understanding of the mechanisms of Xist RNA induction, and, separately, the establishment and maintenance of gene silencing on the inactive-X by Xist RNA during imprinted and random X-inactivation.
Effect of Climate and Land-Use Changes on a Threatened Forest Resident Bird
Authors: Zhao Yuze , Lu Shuai , Hua Junqin , Liu Zhengxiao , Xu Jiliang
Published: February 2024

Endangered species are being threatened by climate and land-use changes. However, the relative effects of climate and land-use changes on forest resident birds remain unclear. In this study, we employed an ecological niche model to forecast the potential influence of climate and land-use changes on Reeves’s pheasant (Syrmaticus reevesii), while taking into account topographical limitations. We observed that climate and land-use changes would have a remarkably negative impact on Syrmaticus reevesii, and climate change would play a dominant role. Consequently, the potential distribution range of Syrmaticus reevesii would sharply decrease, and this species may face a significant risk of extinction in 2070. In addition, we found that the area change predicted by climate and land-use changes would be significantly lower inside the China Nature Reserve Network (NNR) than outside the NNR. However, less than 15% of the area suitable for Syrmaticus reevesii has been covered by NNR. Furthermore, our results showed that the response of Syrmaticus reevesii to climate and land-use changes largely depended on topographical factors, and Syrmaticus reevesii would face greater impacts from climate and land-use changes under topographical limitations. Taken together, we highlight that it is imperative to adjust and develop nature reserve networks and conservation strategies to cope with rapid global environmental change.
Response of Individual-Tree Aboveground Biomass to Spatial Effects in Pinus kesiya var. langbianensis Forests by Stand Origin and Tree Size
Authors: Liu Chunxiao , Wu Yong , Zhang Xiaoli , Luo Hongbin , Yu Zhibo , Liu Zihao , Li Wenfang , Fan Qinling , Ou Guanglong
Published: February 2024

To enhance forest carbon sequestration capacity, it is important to optimize forest structure by revealing the spatial effects of the aboveground biomass of individual trees, with particular emphasis on stand origin and tree size. Here, 0.3 ha clear-cut plots of Pinus kesiya var. langbianensis forest were selected in a typical plantation and natural stand. Then, the ordinary least squares model and spatial regression models were used to analyze the different responses between spatial position and individual tree biomass based on the stand origin and diameter at breast height (DBH) of the tree. Our study shows the following: (1) The spatial effect produced a stronger response in the natural stand than in the plantation. The amount of change in the adjusted R-squared (ΔRadj2) of tree component totaled 0.34 and 0.57 for Pinus kesiya var. langbianensis and other trees in the natural stand, compared to only 0.2 and 0.42 in the plantation; (2) Spatial effects had a stronger impact on the accuracy of the fit for the crown (ΔRadj2 = 0.52) compared to the wood and bark (ΔRadj2 = 0.03) in the plantation, and there were no significant differences in the natural stand (ΔRadj2 = 0.42, ΔRadj2 = 0.43); (3) When DBH reached a certain size, the impact of spatial effect for the crown showed a significant change from positive to negative. The sizes of DBH were 19.5 cm, 14 cm and 34.6 cm, 19 cm for branches of Pinus kesiya var. langbianensis and other tree species in the plantation and natural stand, and were 20.3 cm and 31.4 cm for the foliage of Pinus kesiya var. langbianensis. Differences in stand structure led to varied responses in the biomass of tree components to spatial effects.
Pedodiversity and Organic Matter Dynamics in the North Apennines (Italy): Relationships among Soil Types, Biodiversity, and Ecological Functionality
Authors: Vittori Antisari Livia , De Feudis Mauro , Trenti William , Falsone Gloria , Puliga Federico , Zambonelli Alessandra , Tabanelli Giulia , Gardini Fausto
Published: February 2024

Pedodiversity is generally neglected in studies concerning soil organic carbon (SOC). Therefore, this investigation aimed to explore the effect of soil types on the following: (1) soil processes related to organic matter (OM) dynamics along the profile; and (2) the microbial community and functionality within the uppermost horizon. Humic Dystrudepts (HD), Typic Dystrudepts (TD), and Humic Lithic Dystrudepts (HLD) were selected in beech forests of the Apennine ridge in the Emilia-Romagna Region (Italy). Soils were sampled by horizons until parent material, and physico-chemical and functional analyses were performed. The results showed that both HD and HLD soils had a higher SOC accumulation than TD, particularly within the deeper horizons. Such accumulation might be due to the lower turnover rate of soil OM forms, namely fulvic acid-like substances, humic acid-like substances, and non-extractable OM. Noteworthy, the A horizons showed slight differences in SOC among the soil types, suggesting similar SOC decomposition processes. This fact was confirmed by the lack of differences in microbial DNA-based diversity and functionality. This study highlighted the importance of combining pedodiversity and microbial diversity for a wider perspective on SOC dynamics.
Unveiling the Essential Role of Green Spaces during the COVID-19 Pandemic and Beyond
Authors: Ciesielski Mariusz , Gołos Piotr , Stefan Fruzsina , Taczanowska Karolina
Published: February 2024

The COVID-19 pandemic highlighted the essential role of urban and rural green spaces for societies coping with global public health crisis. During this particular time, a significant body of research was devoted to human–nature relationships, as well as the use and importance of green spaces, both from the management and visitors’ perspectives, along with the vital role of nature in human health and wellbeing. Furthermore, the pandemic experience induced new paradigms in spatial and urban planning, along with the management of forest and protected areas seeing the crucial role of green spaces in shaping long-term socio-environmental resilience and sustainability. Thus, after the official end of the pandemic, our study aimed to provide a systematic review of the international research related to green spaces within the context of the COVID-19 pandemic, focusing on those published between 2020 and 2023. The literature search within SCOPUS and Web of Science databases was conducted on 16 May 2023. A dataset of 161 articles was analyzed using a two-stage analysis. In the first stage, screening based on the title, abstract, and keywords was carried out. In the second stage, a detailed full text analysis was carried out, resulting in a final dataset of 66 articles related to the scope of this review. This article gives an in-depth methodological and conceptual overview, also referring to the applied research and management context related to green spaces in urban and rural environments. It concludes with lessons learned and poses open questions for future research related to green space planning and management. The literature review shows that institutions managing green spaces in cities and forests are facing new challenges. These include pursuing sustainable management policies in cities, ensuring equitable access to urban green space and community participation in the decision-making process, adapting suburban forest management to social expectations, and the recreational development of forest areas taking into account social needs and ecosystem sustainability.
The Pulses of Soil CO2 Emission in Response to Rainfall Events in Central Siberia: Revisiting the Overall Frost-Free Season CO2 Flux
Authors: Makhnykina Anastasia V. , Vaganov Eugene A. , Panov Alexey V. , Koshurnikova Nataly N. , Prokushkin Anatoly S.
Published: February 2024

Boreal forests nowadays act as a sink for atmospheric carbon dioxide; however, their sequestration capacity is highly sensitive to weather conditions and, specifically to ongoing climate warming. Extreme weather events such as heavy rainfalls or, conversely, heat waves during the growing season might perturb the ecosystem carbon balance and convert them to an additional CO2 source. Thus, there is an urgent need to revise ecosystem carbon fluxes in vast Siberian taiga ecosystems as influenced by extreme weather events. In this study, we focused on the soil CO2 pulses appearing after the rainfall events and quantification of their input to the seasonal cumulative CO2 efflux in the boreal forests in Central Siberia. Seasonal measurements of soil CO2 fluxes (both soil respiration and net soil exchange) were conducted during three consecutive frost-free seasons using the dynamic chamber method. Seasonal dynamics of net soil exchange fluxes demonstrated positive values, reflecting that soil respiration rates exceeded CO2 uptake in the forest floor vegetation layer. Moreover, the heavy rains caused a rapid pulse of soil emissions and, as a consequence, the release of additional amounts of CO2 from the soil into the atmosphere. A single rain event may cause a 5–11-fold increase of the NSE flux compared to the pre-rainfall values. The input of CO2 pulses to the seasonal cumulative efflux varied from near zero to 39% depending on precipitation patterns of a particular season. These findings emphasize the critical need for more frequent measurements of soil CO2 fluxes throughout the growing season which capture the CO2 pulses induced by rain events. This approach has inevitable importance for the accurate assessment of seasonal CO2 soil emissions and adequate predictions of response of boreal pine forests to climatic changes.
Spruce Protection against Ips typographus with Anti-Attractant Blend of Tree-Based Semiochemicals: From Small Experimental Plots to Stand Scales
Authors: Jakuš Rastislav , Trubin Aleksei , Singh Vivek Vikram , Zabihi Khodabakhsh , Jirošová Anna , Modlinger Roman , Majdák Andrej , Korolyova Nataliya , Moliterno Antonioni Acacio Campos , Kašpar Jaroslav , Slavík Martin , Surový Peter , Turčáni Marek , Schlyter Fredrik
Published: February 2024

Tree-killing bark beetles require rapid management, such as anti-attractants, to stop the enlargement of attack hot-spots. We tested two newer anti-attractant blends, both without verbenone and one with the addition of trans-4-thujanol, in traps against standard pheromone baits for inhibition of catch. Both blends provided effective catch reduction (>95%). We also tested these anti-attractant blends in tree protection experiments for two years. We had experimental plots with a center of an anti-attractant protected tree zone, with no traditional control area, but we followed tree kills in 10 m wide concentric rings to 100 m. In 2020, we had 12 plots, and 9 plots in 2021. Monitoring by low-strength pheromone traps followed beetle flight averaging 300/trap during the shorter period, August 2020, and 5000/trap during the longer period, May to August 2021. The blends of anti-attractants were 100% effective in avoiding tree mortality in both treated trees and their surroundings. There were no bark beetle attacks on any treated trees, and there was zero tree mortality up to 19 m in 2020, and up to 30 m in 2021, thus full protection to circa 20 m. The density of killed trees then increased from close to zero, over 20 to 50 m, reaching a level of ca 30 (trees/ha) then declined. The spatial pattern of tree mortality on our experimental plots was highly heterogeneous and individual 10m-ring data points on tree kill density could not be statistically separated. In contrast, a non-linear regression model showed a continuous increase of attacks over the distance from the center to a peak ca 60m, followed by a decline. This model agrees partly with the only similar study in the literature, but importantly, it does not give a peak of kill density at distances between the first and second rings close to treatment zones. Such patterns of close-quarter kills have been observed as a “switching” of attack in this and other scolytid systems manipulated by anti-attractants, but not in the present study, likely due to the elimination of verbenone from our blends.
Ipomoea cairica (L.) from Mangrove Wetlands Acquired Salt Tolerance through Phenotypic Plasticity
Authors: Zou Jiatong , Yuan Benqi , Li Weihua , Xie Xiaoting , Chen Minghao , Xiong Tiantian
Published: February 2024

Palmate-leaved morning glory (Ipomoea cairica (L.) Sweet) is a fast-growing perennial herbaceous twining vine that was recently discovered to invade mangrove wetlands in China. To understand the mechanism of its successful invasion, the salt tolerance of a coastal ecotype from Zhuhai and a terrestrial ecotype from Guangzhou were compared under salt stress. The morphological, physiological, and biochemical parameters related to growth, ion homeostasis, photosynthetic pigments, chlorophyll fluorescence parameters, oxidative stress, and apoptosis were measured in both ecotypes. Monitoring apoptosis showed that the protoplasts of the coastal ecotype underwent apoptosis and were later compared with those of the terrestrial ecotype. The coastal ecotype was also found to have higher regenerated stems; less water loss, sodium (Na+) uptake, and membrane damage; higher salt gland density and area; and better photosynthetic performance than the terrestrial ecotype. The coastal ecotype probably prevented salt-related damage by reducing its water loss and secreting excess Na+ through its lower stomatal density and higher density and area of salt glands. The coastal ecotype also maintained a better balance of Na+, potassium ions, nitrogen, and phosphorus under salt stress. Moreover, the coastal ecotype had higher activities of antioxidant enzymes, including superoxide dismutase, peroxidase, and catalase, and a higher content of non-enzymatic antioxidants, including proline and anthocyanins, which indicate a stronger antioxidant ability. Our results suggest that the coastal ecotype adapts to a higher salt tolerance than the terrestrial ecotype by enhancing its exclusion of salt, adjusting its osmolytes, and through photosynthetic efficiency, which could explain its successful invasion in the mangrove wetland ecosystem.
Lutein from Microalgae: An Industrial Perspective of Its Production, Downstream Processing, and Market
Authors: Iwamoto Hissashi , Soccol Carlos Ricardo , Molina-Aulestia Denisse Tatiana , Cardoso Juliana , de Melo Pereira Gilberto Vinícius , de Souza Vandenberghe Luciana Porto , Manzoki Maria Clara , Ambati Ranga Rao , Ravishankar Gokare Aswathanarayana , de Carvalho Júlio Cesar
Published: February 2024

Lutein, a yellow xanthophyll carotenoid, is increasingly recognized for its nutraceutical benefits, particularly in protecting the retina’s macula from age-related degeneration. Microalgae are a promising source of lutein, which can be a primary product or a coproduct in biorefineries. Certain microalgae exhibit lutein levels (up to 1.7%) surpassing those of common dietary sources like kale, spinach, and egg yolk (approximately 0.7–0.9%). Predominantly associated with photosystem II’s light-harvesting complex, lutein is crucial in photosynthesis and cellular defense. However, being quantitatively minor among cellular constituents, lutein necessitates specialized processing for efficient extraction. Although ubiquitous in microalgae, it is not as easily inducible as β-carotene and astaxanthin in Dunaliella salina and Haematococcus pluvialis, respectively. Currently, microalgal lutein production predominantly occurs at the bench scale, presenting challenges in scaling up. Factors like culture medium significantly influence biomass and lutein yields in industrial production, while downstream processing requires cost-effective, food-grade solvent extraction techniques. This review delves into contemporary methods and innovative progress in microalgal lutein production, emphasizing industrial-scale processes from biomass cultivation to final product formulation. A conceptual industrial process proposed in this review shows that two 10 m3 photobioreactors could produce 108 kg dry mass for Chlorella minutissima, which can be processed into approximately 616 g of lutein extract, or over 6000 capsules of finished nutraceutical daily. Despite lutein production via microalgae being in nascent stages at large scales, existing research provides a solid foundation for well-informed scale-up endeavors.
Distributed Mobility Management Support for Low-Latency Data Delivery in Named Data Networking for UAVs
Authors: Bellaj Mohammed , Naja Najib , Jamali Abdellah
Published: February 2024

Named Data Networking (NDN) has emerged as a promising architecture to overcome the limitations of the conventional Internet Protocol (IP) architecture, particularly in terms of mobility, security, and data availability. However, despite the advantages it offers, producer mobility management remains a significant challenge for NDN, especially for moving vehicles and emerging technologies such as Unmanned Aerial Vehicles (UAVs), known for their high-speed and unpredictable movements, which makes it difficult for NDN to maintain seamless communication. To solve this mobility problem, we propose a Distributed Mobility Management Scheme (DMMS) to support UAV mobility and ensure low-latency content delivery in NDN architecture. DMMS utilizes decentralized Anchors to forward proactively the consumer’s Interest packets toward the producer’s predicted location when handoff occurs. Moreover, it introduces a new forwarding approach that combines the standard and location-based forwarding strategy to improve forwarding efficiency under producer mobility without changing the network structure. Using a realistic scenario, DMMS is evaluated and compared against two well-known solutions, namely MAP-ME and Kite, using the ndnSIM simulations. We demonstrate that DMMS achieves better results compared to Kite and MAP-ME solutions in terms of network cost and consumer quality-of-service metrics.
CROWDMATCH: Optimizing Crowdsourcing Matching through the Integration of Matching Theory and Coalition Games
Authors: Adesokan Adedamola , Kinney Rowan , Tsiropoulou Eirini Eleni
Published: February 2024

This paper tackles the challenges inherent in crowdsourcing dynamics by introducing the CROWDMATCH mechanism. Aimed at enabling crowdworkers to strategically select suitable crowdsourcers while contributing information to crowdsourcing tasks, CROWDMATCH considers incentives, information availability and cost, and the decisions of fellow crowdworkers to model the utility functions for both the crowdworkers and the crowdsourcers. Specifically, the paper presents an initial Approximate CROWDMATCH mechanism grounded in matching theory principles, eliminating externalities from crowdworkers’ decisions and enabling each entity to maximize its utility. Subsequently, the Accurate CROWDMATCH mechanism is introduced, which is initiated by the outcome of the Approximate CROWDMATCH mechanism, and coalition game-theoretic principles are employed to refine the matching process by accounting for externalities. The paper’s contributions include the introduction of the CROWDMATCH system model, the development of both Approximate and Accurate CROWDMATCH mechanisms, and a demonstration of their superior performance through comprehensive simulation results. The mechanisms’ scalability in large-scale crowdsourcing systems and operational advantages are highlighted, distinguishing them from existing methods and highlighting their efficacy in empowering crowdworkers in crowdsourcer selection.
The Effects of Different Feed Ration Levels on Growth, Welfare Rating, and Early Maturation in Juvenile Atlantic Salmon (Salmo salar)
Authors: Imsland Albert Kjartan Dagbjartarson , Methúsalemsson Hjörtur
Published: February 2024

To investigate the possible effect of different feed ration levels on the growth, welfare, and early maturation of juvenile Atlantic salmon, 450 salmon parr with a mean (±standard error) initial weight of 51.6 g (±0.8) were reared in triplicate under three different feed ration levels for five months. The control group (100r) was fed every day, the 50r group was fed every other day, and the 33r group was fed every third day. In every group, 75 fish (half of the group) were individually tagged for monitoring of growth. The number of fin wounds was used as the welfare indicator, and to inspect the development of maturation, all fish were euthanized, and development of the gonads was monitored by visual inspection at the termination of the trial. The control group (100r) showed a significantly higher specific growth rate (0.90% day−1) compared to the lower fed groups (50r, 0.67% day−1 and 33r, 0.49% day−1); however, the growth difference was 21–24% less than expected solely on the difference in the amount of feed given to each group. The 100r group showed the highest welfare rating, and the 33r group the lowest possible, indicating more aggressive behaviour and fin biting due to feed restriction in the 33r group. No difference (p > 0.45) was found in the development of maturation in females, but the combined numbers of males in maturity stages 2–5 showed an overall trend towards slower maturation in the 33r group compared to the 100r group. Although the present findings on the development of sexual maturation were subtle due to the limited time frame of the trial, the findings offer a foundation for future investigation into the relationship between the feed ration level and the development of sexual maturation in the rearing of juvenile Atlantic salmon.
CFD Analysis of Ultra-High-Performance Concrete Rheological Tests
Authors: Jirout Tomáš , Krupica Adam , Kolomijec Alexandr
Published: February 2024

This study connects and compares the results from two different rheological measurement techniques, namely, the slump test and rotational rheometry, on UHPC (Ultra-High-Performance Concrete) through the use of commercially available numerical simulation software ANSYS Fluent 2022 R2. The workability and resulting mechanical properties of the UHPC (a material used in construction) are highly dependent on its rheology and, hence, also on the composition and level of homogeneity of the assessed mixture. It is generally understood that the most suitable rheological model for concrete mixtures is the Hershel–Bulkley model. However, obtaining reliable rheological data is complicated as the wide-gap rotational rheometers developed for concrete show bias in their measurements even on precise laboratory equipment, while common industrial tests, such as the slump test, do not produce the usual shear rate–shear stress relation and, hence, do not allow for more complex analysis. Recently, a new methodology for the rheological measurement of non-Newtonian fluids that utilises a simple power input–rotation speed measurement was published. However, in this study, only model liquids were evaluated, and the method was not validated for more complex fluids such as pastes. Therefore, it was the goal of this study to show this method’s suitability for fine pastes through a comparison with the slump test, using numerical simulation.
Preparation of Sweet Potato Porous Starch by Marine Dextranase and Its Adsorption Characteristics
Authors: Hao Yue , Liu Mingwang , Ni Hao , Bai Yue , Hao Qingfang , Zhang Lei , Kang Xinxin , Lyu Mingsheng , Wang Shujun
Published: February 2024

Dextranase (EC 3.2.1.11) is primarily applied in food, sugar, and pharmaceutical industries. This study focuses on using a cold shock Escherichia coli expression system to express marine dextranase SP5-Badex; enzyme activity increased about 2.2-fold compared to previous expression. This enzyme was employed to produce sweet potato porous starch, with special emphasis on the pore size of the starch. The water and oil adsorption rates of the porous starch increased by 1.43 and 1.51 times, respectively. Extensive Fourier transform infrared spectroscopy and X-ray diffraction revealed that the crystal structure of the sweet potato starch was unaltered by enzymatic hydrolysis. The adsorption capacities of the porous starch for curcumin and proanthocyanidins were 9.59 and 12.29 mg/g, respectively. Notably, the stability of proanthocyanidins was significantly enhanced through their encapsulation in porous starch. After 2.5 h of ultraviolet irradiation, the free radical scavenging rate of the encapsulated proanthocyanidins remained at 95.10%. Additionally, after 30 days of sunlight exposure, the free radical scavenging rate of the encapsulated proanthocyanidins (84.42%) was significantly higher than that (24.34%) observed in the control group. These research findings provide substantial experimental evidence for preparing sweet potato porous starch using marine dextranase.
Effect of Fertilization on Phenolics of Rapeseeds and Their Antioxidant Potential
Authors: Amarowicz Ryszard , Cwalina-Ambroziak Bożena , Janiak Michał Adam , Damszel Marta , Stępień Arkadiusz , Sulewska Katarzyna , Karamać Magdalena , Penkacik Kamila
Published: February 2024

Three varieties of rapeseed (Castilla, California, and Nelson F1) were cultivated using medium–intensive (control), intensive, and economical (spare) technologies with different nitrogen and sulfur fertilization techniques. The antioxidant potential of rapeseeds was investigated using ABTS, FRAP, and DPPH assays. The content of total phenolic compounds was determined using the Folin–Ciocalteu phenol reagent. The profile of phenolic compounds was determined using high-performance liquid chromatography (HPLC). Diversifying fertilization in various ways influenced the content of phenolic compounds in extracts of rapeseed. In extracts from the Nelson F1 rapeseeds, intensive cultivation resulted in a lower content of phenolic compounds compared to the control group. Economic fertilization reduced the content of phenolic compounds in seeds from the California variety. HPLC chromatograms of the extracts were characterized by the presence of five (California and Castilla) and six (Nelson F1) main phenolic compounds. Two compounds were identified as sinapine and sinapic acid; others were classified as derivatives of sinapic acid. The effect of fertilization on the antioxidant activity of the seeds and their extracts varied depending on the plant variety and antioxidant assay. For the Castilla and California varieties, no differences were found in the results of the ABTS assay. The antiradical activity against ABTS•+ of extracts from the Nelson F1 intensive and spare cultivated seeds was higher than that of extracts from control seeds. The FRAP values of extracts/seeds from the Castilla variety cultivated using different methods did not differ significantly. The results of the DPPH assay were not affected by fertilization in the case of extracts from the California and Castilla varieties. However, the extracts from spare cultivated seeds of Nelson F1 exhibited stronger antiradical activity against DPPH•. These findings highlight the complex relationship between fertilization practices, phenolic compound accumulation, and antioxidant activity in rapeseed. Integrating varietal traits and cultivation practices is crucial for optimizing the nutritional benefits of rapeseed.
Relevance of Extending FGFR3 Gene Analysis in Osteochondrodysplasia to Non-Coding Sequences: A Case Report
Authors: Ouedraogo Zangbéwendé Guy , Janel Caroline , Janin Alexandre , Millat Gilles , Langlais Sarah , Pontier Bénédicte , Biard Marie , Lepage Mathis , Francannet Christine , Laffargue Fanny , Creveaux Isabelle
Published: February 2024

Skeletal dysplasia, also called osteochondrodysplasia, is a category of disorders affecting bone development and children’s growth. Up to 552 genes, including fibroblast growth factor receptor 3 (FGFR3), have been implicated by pathogenic variations in its genesis. Frequently identified causal mutations in osteochondrodysplasia arise in the coding sequences of the FGFR3 gene: c.1138G>A and c.1138G>C in achondroplasia and c.1620C>A and c.1620C>G in hypochondroplasia. However, in some cases, the diagnostic investigations undertaken thus far have failed to identify the causal anomaly, which strengthens the relevance of the diagnostic strategies being further refined. We observed a Caucasian adult with clinical and radiographic features of achondroplasia, with no common pathogenic variant. Exome sequencing detected an FGFR3(NM_000142.4):c.1075+95C>G heterozygous intronic variation. In vitro studies showed that this variant results in the aberrant exonization of a 90-nucleotide 5′ segment of intron 8, resulting in the substitution of the alanine (Ala359) for a glycine (Gly) and the in-frame insertion of 30 amino acids. This change may alter FGFR3’s function. Our report provides the first clinical description of an adult carrying this variant, which completes the phenotype description previously provided in children and confirms the recurrence, the autosomal-dominant pathogenicity, and the diagnostic relevance of this FGFR3 intronic variant. We support its inclusion in routinely used diagnostic tests for osteochondrodysplasia. This may increase the detection rate of causal variants and therefore could have a positive impact on patient management. Finally, FGFR3 alteration via non-coding sequence exonization should be considered a recurrent disease mechanism to be taken into account for new drug design and clinical trial strategies.
Male Pedigree Toolbox: A Versatile Software for Y-STR Data Analyses
Authors: Ralf Arwin , van Wersch Bram , Montiel González Diego , Kayser Manfred
Published: February 2024

Y-chromosomal short tandem repeats (Y-STRs) are widely used in forensic, genealogical, and population genetics. With the recent increase in the number of rapidly mutating (RM) Y-STRs, an unprecedented level of male differentiation can be achieved, widening and improving the applications of Y-STRs in various fields, including forensics. The growing complexity of Y-STR data increases the need for automated data analyses, but dedicated software tools are scarce. To address this, we present the Male Pedigree Toolbox (MPT), a software tool for the automated analysis of Y-STR data in the context of patrilineal genealogical relationships. The MPT can estimate mutation rates and male relative differentiation rates from input Y-STR pedigree data. It can aid in determining ancestral haplotypes within a pedigree and visualize the genetic variation within pedigrees in all branches of family trees. Additionally, it can provide probabilistic classifications using machine learning, helping to establish or prove the structure of the pedigree and the level of relatedness between males, even for closely related individuals with highly similar haplotypes. The tool is flexible and easy to use and can be adjusted to any set of Y-STR markers by modifying the intuitive input file formats. We introduce the MPT software tool v1.0 and make it publicly available with the goal of encouraging and supporting forensic, genealogical, and other geneticists in utilizing the full potential of Y-STRs for both research purposes and practical applications, including criminal casework.
Identification of Candidate Genes and Pathways Linked to the Temperament Trait in Sheep
Authors: Romaniuk Estefanía , Vera Brenda , Peraza Pablo , Ciappesoni Gabriel , Damián Juan Pablo , Van Lier Elize
Published: February 2024

Temperament can be defined as the emotional variability among animals of the same species in response to the same stimulus, grouping animals by their reactivity as nervous, intermediate, or calm. Our goal was to identify genomic regions with the temperament phenotype measured by the Isolation Box Test (IBT) by single-step genome-wide association studies (ssGWAS). The database consisted of 4317 animals with temperament records, and 1697 genotyped animals with 38,268 effective Single Nucleotide Polymorphism (SNP) after quality control. We identified three genomic regions that explained the greatest percentage of the genetic variance, resulting in 25 SNP associated with candidate genes on chromosomes 6, 10, and 21. A total of nine candidate genes are reported for the temperament trait, which is: PYGM, SYVN1, CAPN1, FADS1, SYT7, GRID2, GPRIN3, EEF1A1 and FRY, linked to the energetic activity of the organism, synaptic transmission, meat tenderness, and calcium associated activities. This is the first study to identify these genetic variants associated with temperament in sheep, which could be used as molecular markers in future behavioral research.
Complete Chloroplast Genomes of Four Oaks from the Section Cyclobalanopsis Improve the Phylogenetic Analysis and Understanding of Evolutionary Processes in the Genus Quercus
Authors: Wang Ling-Ling , Li Yu , Zheng Si-Si , Kozlowski Gregor , Xu Jin , Song Yi-Gang
Published: February 2024

Quercus is a valuable genus ecologically, economically, and culturally. They are keystone species in many ecosystems. Species delimitation and phylogenetic studies of this genus are difficult owing to frequent hybridization. With an increasing number of genetic resources, we will gain a deeper understanding of this genus. In the present study, we collected four Quercus section Cyclobalanopsis species (Q. poilanei, Q. helferiana, Q. camusiae, and Q. semiserrata) distributed in Southeast Asia and sequenced their complete genomes. Following analysis, we compared the results with those of other species in the genus Quercus. These four chloroplast genomes ranged from 160,784 bp (Q. poilanei) to 161,632 bp (Q. camusiae) in length, with an overall guanine and cytosine (GC) content of 36.9%. Their chloroplast genomic organization and order, as well as their GC content, were similar to those of other Quercus species. We identified seven regions with relatively high variability (rps16, ndhk, accD, ycf1, psbZ—trnG-GCC, rbcL—accD, and rpl32—trnL-UAG) which could potentially serve as plastid markers for further taxonomic and phylogenetic studies within Quercus. Our phylogenetic tree supported the idea that the genus Quercus forms two well-differentiated lineages (corresponding to the subgenera Quercus and Cerris). Of the three sections in the subgenus Cerris, the section Ilex was split into two clusters, each nested in the other two sections. Moreover, Q. camusiae and Q. semiserrata detected in this study diverged first in the section Cyclobalanopsis and mixed with Q. engleriana in the section Ilex. In particular, 11 protein coding genes (atpF, ndhA, ndhD, ndhF, ndhK, petB, petD, rbcL, rpl22, ycf1, and ycf3) were subjected to positive selection pressure. Overall, this study enriches the chloroplast genome resources of Quercus, which will facilitate further analyses of phylogenetic relationships in this ecologically important tree genus.
Genome-Wide Detection for Runs of Homozygosity in Baoshan Pigs Using Whole Genome Resequencing
Authors: Li Wenjun , Wu Xudong , Xiang Decai , Zhang Wei , Wu Lingxiang , Meng Xintong , Huo Jinlong , Yin Zongjun , Fu Guowen , Zhao Guiying
Published: February 2024

Baoshan pigs (BS) are a local breed in Yunnan Province that may face inbreeding owing to its limited population size. To accurately evaluate the inbreeding level of the BS pig population, we used whole-genome resequencing to identify runs of homozygosity (ROH) regions in BS pigs, calculated the inbreeding coefficient based on pedigree and ROH, and screened candidate genes with important economic traits from ROH islands. A total of 22,633,391 SNPS were obtained from the whole genome of BS pigs, and 201 ROHs were detected from 532,450 SNPS after quality control. The number of medium-length ROH (1–5 Mb) was the highest (98.43%), the number of long ROH (>5 Mb) was the lowest (1.57%), and the inbreeding of BS pigs mainly occurred in distant generations. The inbreeding coefficient FROH, calculated based on ROH, was 0.018 ± 0.016, and the FPED, calculated based on the pedigree, was 0.027 ± 0.028, which were positively correlated. Forty ROH islands were identified, containing 507 genes and 891 QTLs. Several genes were associated with growth and development (IGFALS, PTN, DLX5, DKK1, WNT2), meat quality traits (MC3R, ACSM3, ECI1, CD36, ROCK1, CACNA2D1), and reproductive traits (NPW, TSHR, BMP7). This study provides a reference for the protection and utilization of BS pigs.
Could MRONJ Be Related to Osimertinib Monotherapy in Lung Cancer Patients after Denosumab Suspension?
Authors: Forte Marta , d’Amati Antonio , Limongelli Luisa , Corsalini Massimo , Favia Gianfranco , Ingravallo Giuseppe , Barile Giuseppe , Capodiferro Saverio
Published: February 2024

Background: Medication-related osteonecrosis of the jaws is the most frequent complication in patients treated or in therapy with antiresorptive/antiangiogenetic drugs. The list of medications possibly related to MRONJ onset is constantly growing; we aimed to report on a third-generation epidermal growth factor receptor tyrosine kinase inhibitor (Osimertinib) as possibly responsible for bilateral maxillary necrosis onset in the herein-described case. Methods: In June 2023, an oncologic patient with two different maxillary bone exposures was referred to our attention. His medical history revealed a two-year Denosumab regimen along with Osimertinib, the latter not suspended before teeth extractions. The clinicians performed a sequestrum removal and bone debridement after three cycles of antibiotic therapy. Results: Histologic examinations confirmed the clinical diagnosis of MRONJ excluding a metastatic occurrence, while complete mucosal healing was achieved after 15 days. Conclusions: The patient suspended Denosumab for more than six months before teeth extraction for MRONJ prevention; hence, failure to discontinue Osimertinib led us to consider it a possible etiological factor. From a literature analysis, only one case has already been published reporting a possible Osimertinib-related occurrence of MRONJ in lung cancer patients. Our case is a further report that could be intended as an alert both for oncologists and dentists to share decisions about the oral management of such patients together, also informing them about this possible risk. Also, this report could trigger in the scientific community the necessity to evaluate further guidelines for similar doubtful cases in which the drug interaction, the mono-suspension, and the possible removable prosthesis-related additional trauma should be considered causes or con-causes.
The Association between Message Framing and Intention to Vaccinate Predictive of Hepatitis A Vaccine Uptake
Authors: Satybaldiyeva Nora , Martinez Lourdes S. , Cooper Brittany , Oren Eyal
Published: February 2024

As ongoing, sporadic outbreaks of hepatitis A virus (HAV) infections present public health challenges, it is critical to understand public perceptions about HAV, especially regarding vaccination. This study examines whether message framing changes the intention to vaccinate against HAV and self-reported vaccine behavior. Using a randomized controlled trial (N = 472) in February 2019 via Amazon Mechanical Turk, participants were randomized to one of four HAV vaccination message groups or a no-message control group. The message groups varied in their emphasis on the nature of outcomes (gain versus loss) and for whom (individual versus collective). The message frames were compared by intention to vaccinate, differences in message characteristics, and behavioral determinants. There was no difference in intention to vaccinate between gain- versus loss-framed messages (MD = 0.1, 95% CI = −0.1, 0.3) and individual- versus collective-framed messages (MD = 0.1, 95% CI = −0.1, 0.3). The intention to vaccinate against HAV in the no-message control group was very similar to that in the message groups. However, gain-framed messages were rated more positively in valence than loss-framed messages (MD = −0.5, 95% CI = −0.7, −0.3), which may be helpful for cultivating a positive public perception of HAV vaccination. The study also highlights the importance of comparing message frames to a no-message control in designing health communication messaging promoting HAV vaccination.