bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessment of Efficacy of Algerian Propolis against the Parasitic Mite Varroa destructor and Safety for Honey Bees by Spray Treatment
Authors: Ayad Ahmed Sabri , Benchaabane Samia , Daas Tarek , Smagghe Guy , Loucif-Ayad Wahida
Published: January 2024

Varroa destructor is an ectoparasitic mite and is considered one of the most important causes of honey bee population loss. In the last years, substances of botanical origin have emerged as natural alternatives to diminish the mite population levels. Propolis is a natural product and is used by honey bees for multiple tasks, including protection from pathogens and parasites, and varroacidal activity of propolis extracts has been shown. In this study, we investigated the potential of propolis, collected by native Algerian honey bee subspecies (Apis mellifera intermissa and A. m. sahariensis) in different locations in Algeria and extracted by ultrasound, to control mites of V. destructor and tested the safety for the honey bees. The most important results were that the best propolis extracts at 10% killed 100% of the Varroa mites within 3–4 h in a Petri dish assay. In addition, when we sprayed A. m. intermissa bees infested with Varroa mites with a 10% concentration in a mini-hive setup, we scored a high mite mortality of 85–87% with the best propolis extracts, and importantly, there was no mortality in the bees. Our data demonstrated that propolis extracts in Algeria could be used in honey bee colonies by spraying against Varroa mite infestations, which may develop as an easy method for local beekeepers to control Varroa in their hives. Further research should investigate the mechanism of action.
Comment on Schuderer et al. Risk Factors for Flap Loss: Analysis of Donor and Recipient Vessel Morphology in Patients Undergoing Microvascular Head and Neck Reconstructions. J. Clin. Med. 2023, 12, 5206
Authors: Bar Ori , Abu El Naaj Imad
Published: January 2024

We have read with much interest the study by Schuderer et al [...]
The Influences of Chemical Modifications on the Structural, Mechanical, Tribological and Adhesive Properties of Oriented UHMWPE Films
Authors: Dayyoub Tarek , Kolesnikov Evgeniy , Filippova Olga V. , Kaloshkin Sergey D. , Telyshev Dmitry V. , Maksimkin Aleksey V.
Published: January 2024

Preparing a friction pair “polymer-metal” using improved polymeric composites is contemplated a complicated task due to the inert surface of the polymer. Gluing polymer composites with improved mechanical and tribological properties on metals and saving their unique properties at the same time is considered the best way to prepare slide bearing products based on polymer/metal. In this work, ultraviolet initiation is used after a process of mixed acid pre-treatment. The surface of highly oriented films based on ultra-high molecular weight polyethylene (UHMWPE)/graphene nanoplatelets (GNP) is grafted with nanocellulose. The grafting treatment is analyzed using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and contact angle measurements. Mechanical T-peel tests showed that the peel strength for the treated UHMWPE films increased by three times, up to 1.9 kg/cm, in comparison to the untreated films. The tensile strength of the treated UHMWPE films decreased by about 6% to 788 MPa. Tribological tests showed that the values of both friction coefficient and wear intensity of the treated UHMWPE films were increased insignificantly, which were 0.172 and 15.43 µm/m·m2, respectively. The prepared adhesive tape based on UHMWPE films, which can withstand a weight of up to 6 kg per 1 cm2 of the bonded surface, has a low coefficient of friction, high wear resistance, and high strength, and is considered a promising material for preparing slide bearing products.
Endoscopic Image Enhancement: Wavelet Transform and Guided Filter Decomposition-Based Fusion Approach
Authors: Moghtaderi Shiva , Yaghoobian Omid , Wahid Khan A. , Lukong Kiven Erique
Published: January 2024

Endoscopies are helpful for examining internal organs, including the gastrointestinal tract. The endoscope device consists of a flexible tube to which a camera and light source are attached. The diagnostic process heavily depends on the quality of the endoscopic images. That is why the visual quality of endoscopic images has a significant effect on patient care, medical decision-making, and the efficiency of endoscopic treatments. In this study, we propose an endoscopic image enhancement technique based on image fusion. Our method aims to improve the visual quality of endoscopic images by first generating multiple sub images from the single input image which are complementary to one another in terms of local and global contrast. Then, each sub layer is subjected to a novel wavelet transform and guided filter-based decomposition technique. To generate the final improved image, appropriate fusion rules are utilized at the end. A set of upper gastrointestinal tract endoscopic images were put to the test in studies to confirm the efficacy of our strategy. Both qualitative and quantitative analyses show that the proposed framework performs better than some of the state-of-the-art algorithms.
Lateral Pectoral Nerve Identification through Ultrasound-Guided Methylene Blue Injection during Selective Peripheral Neurectomy for Shoulder Spasticity: Proposal for a New Procedure
Authors: Zerbinati Paolo , Bemporad Jonathan , Massimiani Andrea , Bianchini Edoardo , Mazzoli Davide , Glorioso Davide , della Vecchia Giuseppe , De Luca Antonio , De Blasiis Paolo
Published: January 2024

Internally rotated and adducted shoulder is a common posture in upper limb spasticity. Selective peripheral neurectomy is a useful and viable surgical technique to ameliorate spasticity, and the lateral pectoral nerve (LPN) could be a potential good target to manage shoulder spasticity presenting with internal rotation. However, there are some limitations related to this procedure, such as potential anatomical variability and the necessity of intraoperative surgical exploration to identify the target nerve requiring wide surgical incisions. This could result in higher post-surgical discomfort for the patient. Therefore, the aim of our study was to describe a modification of the traditional selective peripheral neurectomy procedure of the LPN through the perioperative ultrasound-guided marking of the target nerve with methylene blue. The details of the localization and marking procedure are described, as well as the surgical technique of peripheral selective neurectomy and the potential advantages in terms of nerve localization, surgical precision and patients’ post-surgical discomfort. We suggest that the proposed modified procedure could be a valid technique to address some current limitations and move the surgical treatment of spasticity toward increasingly tailored management due to the ease of nerve identification, the possibility of handling potential anatomical variability and the resulting smaller surgical incisions.
Green Roofs Affect the Floral Abundance and Phenology of Four Flowering Plant Species in the Western United States
Authors: Ruszkowski Kyle Michael , Bousselot Jennifer McGuire
Published: January 2024

This study investigates the potential for green roofs to support pollinator diversity and abundance in urban ecosystems through the altered floral phenology and floral abundance of plants. Floral phenology and the floral abundance of green roof plants are compared to plants grown at grade on the Front Range in Fort Collins, Colorado, and how these changes may affect pollinator biodiversity in urban ecosystems. An independent block design is employed, within one green roof and one ground-level garden, approximately 120 m apart, with replicate plants of 4 species in each garden. Pollinator observations were made weekly during the bloom period for each species. Blue vane traps were used to passively measure pollinator diversity along a transect between the green roof sites and the sites at grade. The total number of flowers per plant is variable between site types, depending on the plant species. However, all species of plants tested bloomed earlier when grown on the green roof than when grown at grade. Pollinator abundance and diversity were low at both site locations. Green roofs may provide foraging opportunities earlier in the season in temperate regions, which can extend the duration of floral foraging opportunities when supported by green infrastructure at grade.
Synthesis and Characterization of Type II Ge-Si Clathrate Films for Optoelectronic Applications
Authors: Kumar Rahul , Kurita Shiori , Ohashi Fumitaka , Iida Tamio , Habuchi Hitoe , Kume Tetsuji
Published: January 2024

Type II inorganic clathrates consist of cage-like structures with open frameworks, and they are considered promising materials due to their unique properties. However, the difficulty of synthesizing phase-pure and continuous films has hindered their application in practical devices. In this report, we demonstrate the synthesis of type II SiGe clathrate films through the thermal decomposition of a Na-deposited amorphous SiGe film on a sapphire substrate in a high vacuum. The as-prepared films of type II SiGe clathrates showed uniform growth and were evaluated for their structural and optical properties. Morphological studies conducted using a scanning electron microscope showed the presence of cracks on the film surface.
A Superhydrophobic Surface on a Superalloy Substrate with Properties of High Mechanical Strength and Self-Cleaning of Carbon Deposition
Authors: Zhang Bingzhen , Chen Yang , Song Jinlong
Published: January 2024

Laser processing is an efficient method for fabricating a superhydrophobic surface and has attracted much attention due to its multifunctionality. However, excessive laser processing, such as laser beam overlap and multiple scans, generates both a thick, brittle recast layer and a thin material thickness, thereby greatly reducing the mechanical strength of the substrate. In addition, there is no report on fabricating a superhydrophobic surface on a superalloy substrate whose application includes a self-cleaning property. This work proposes the fabrication of a superhydrophobic surface on a superalloy substrate with high mechanical strength by optimizing the laser processing parameters including laser power, scanning speed, line spacing, and number of scans. We found that the microstructures required by superhydrophobicity could be constructed with a single laser scan. which could guarantee a minimal loss of the mechanical strength. The fabricated superhydrophobic surface on the superalloy substrate exhibited excellent self-cleaning of carbon deposition, showing good application potential in the aero engine field.
New Water-Soluble (Iminomethyl)benzenesulfonates Derived from Biogenic Amines for Potential Biological Applications
Authors: Kmieciak Anna , Krzemiński Marek P. , Hodii Anastasiia , Gorczyca Damian , Jastrzębska Aneta
Published: January 2024

In this paper, a highly efficient and straightforward method for synthesizing novel Schiff bases was developed by reacting selected biogenic amines with sodium 2-formylbenzene sulfonate and sodium 3-formylbenzene sulfonate. 1H and 13C NMR, IR spectroscopy, and high-resolution mass spectrometry were used to characterize the new compounds. The main advantages of the proposed procedure include simple reagents and reactions carried out in water or methanol and at room temperature, which reduces time and energy. Moreover, it was shown that the obtained water-soluble Schiff bases are stable in aqueous solution for at least seven days. Additionally, the antioxidant and antimicrobial activity of synthesized Schiff bases were tested.
Enhancing Epoxy Composite Performance with Carbon Nanofillers: A Solution for Moisture Resistance and Extended Durability in Wind Turbine Blade Structures
Authors: Ntaflos Angelos , Foteinidis Georgios , Liangou Theodora , Bilalis Elias , Anyfantis Konstantinos , Tsouvalis Nicholas , Tyriakidi Thomais , Tyriakidis Kosmas , Tyriakidis Nikolaos , Paipetis Alkiviadis S.
Published: January 2024

The increasing prominence of glass-fibre-reinforced plastics (GFRPs) in the wind energy industry, due to their exceptional combination of strength, low weight, and resistance to corrosion, makes them an ideal candidate for enhancing the performance and durability of wind turbine blades. The unique properties of GFRPs not only contribute to reduced energy costs through improved aerodynamic efficiency but also extend the operational lifespan of wind turbines. By modifying the epoxy resin with carbon nanofillers, an even higher degree of performance can be achieved. In this work, graphene nanoplatelet (GNP)-enhanced GFRPs are produced through industrial methods (filament winding) and coupons are extracted and tested for their mechanical performance after harsh environmental aging in high temperature and moisture. GNPs enhance the in-plane shear strength of GFRP by 200%, while reducing their water uptake by as much as 40%.
Oxidation Behavior of FeNiCoCrMo0.5Al1.3 High-Entropy Alloy Powder
Authors: Semikolenov Anton , Goshkoderya Mikhail , Uglunts Tigran , Larionova Tatyana , Tolochko Oleg
Published: January 2024

One of the most promising applications of FeNiCoCrMoAl-based high-entropy alloy is the fabrication of protective coatings. In this work, gas-atomized powder of FeNiCoCrMo0.5Al1.3 composition was deposited via high-velocity oxygen fuel spraying. It was shown that in-flight oxidation of the powder influences the coating’s phase composition and properties. Powder oxidation and phase transformations were studied under HVOF deposition, and during continuous heating and prolonged isothermal annealing at 800 °C. Optical and scanning electron microscopy observation, energy dispersive X-ray analysis, X-ray diffraction analysis, thermogravimetric analysis, differential thermal analysis, and microhardness tests were used for study. In a gas-atomized state, the powder consisted of BCC supersaturated solid solution. The high rate of heating and cooling and high oxygen concentration during spraying led to oxidation development prior to decomposition of the supersaturated solid solution. Depleted Al layers of BCC transferred to the FCC phase. An increase in the spraying distance resulted in an increase in α-Al2O3 content; however, higher oxide content does not result in a higher microhardness. In contrast, under annealing, the supersaturated BCC solid solution decomposition occurs earlier than pronounced oxidation, which leads to considerable strengthening to 910 HV.
The Slump Flow of Cementitious Pastes: Simulation vs. Experiments
Authors: Thiedeitz Mareike , Kränkel Thomas , Kartal Deniz , Timothy Jithender J.
Published: January 2024

Understanding the transient properties of cementitious pastes is crucial for construction materials engineering. Computational modeling, particularly through Computational Fluid Dynamics (CFD), offers a promising avenue to enhance our understanding of these properties. However, there are several numerical uncertainties that affect the accuracy of the simulations using CFD. This study focuses on evaluating the accuracy of CFD simulations in replicating slump flow tests for cementitious pastes by determining the impact of the numerical setup on the simulation accuracy and evaluates the transient, viscosity-dependent flows for different viscous pastes. Rheological input parameters were sourced from rheometric tests and Herschel–Bulkley regression of flow curves. We assessed spatial and temporal convergence and compared two regularization methods for the rheological model. Our findings reveal that temporal and spatial refinements significantly affected the final test results. Adjustments in simulation setups effectively reduced computational errors to less than four percent compared to experimental outcomes. The Papanastasiou regularization was found to be more accurate than the bi-viscosity model. Employing a slice geometry, rather than a full three-dimensional cone mesh, led to accurate results with decreased computational costs. The analysis of transient flow properties revealed the effect of the paste viscosity on the time- and shear-dependent flow progress. The study provides an enhanced understanding of transient flow patterns in cementitious pastes and presents a refined CFD model for simulating slump flow tests. These advancements contribute to improving the accuracy and efficiency of computational analyses in the field of cement and concrete flow, offering a benchmark for prospective analysis of transient flow cases.
Application of Structural Equation Modelling to Cybersecurity Risk Analysis in the Era of Industry 4.0
Authors: Gombár Miroslav , Vagaská Alena , Korauš Antonín , Račková Pavlína
Published: January 2024

In the current digital transformation to Industry 4.0, the demands on the ability of countries to react responsibly and effectively to threats in the field of cyber security (CS) are increasing. Cyber safety is one of the pillars and concepts of Industry 4.0, as digitization brings convergence and integration of information technologies (IT) and operational technologies (OT), IT/OT systems, and data. Collecting and connecting a large amount of data in smart factories and cities poses risks, in a broader context for the entire state. The authors focus attention on the issue of CS, where, despite all digitization, the human factor plays a key role—an actor of risk as well as strengthening the sustainability and resilience of CS. It is obvious that in accordance with how the individuals (decision-makers) perceive the risk, thus they subsequently evaluate the situation and countermeasures. Perceiving cyber threats/risks in their complexity as a part of hybrid threats (HT) helps decision-makers prevent and manage them. Due to the growing trend of HT, the need for research focused on the perception of threats by individuals and companies is increasing. Moreover, the literature review points out a lack of methodology and evaluation strategy. This study presents the results of the research aimed at the mathematical modelling of risk perception of threats to the state and industry through the disruption of CS. The authors provide the developed factor model of cyber security (FMCS), i.e., the model of CS threat risk perception. When creating the FMCS, the researchers applied SEM (structural equation modelling) and confirmatory factor analysis to the data obtained by the implementation of the research tool (a questionnaire designed by the authors). The pillars and sub-pillars of CS defined within the questionnaire enable quantification in the perception of the level of risk of CS as well as differentiation and comparison between the analyzed groups of respondents (students of considered universities in SK and CZ). The convergent and discriminant validity of the research instrument is verified, and its reliability is confirmed (Cronbach’s alpha = 0.95047). The influence of the individual pillars is demonstrated as significant at the significance level of α = 5%. For the entire research set N = 964, the highest share of risk perception of CS threats is achieved by the DISRIT pillar (disruption or reduction of the resistance of IT infrastructure).
OCT Retinopathy Classification via a Semi-Supervised Pseudo-Label Sub-Domain Adaptation and Fine-Tuning Method
Authors: Tan Zhicong , Zhang Qinqin , Lan Gongpu , Xu Jingjiang , Ou Chubin , An Lin , Qin Jia , Huang Yanping
Published: January 2024

Conventional OCT retinal disease classification methods primarily rely on fully supervised learning, which requires a large number of labeled images. However, sometimes the number of labeled images in a private domain is small but there exists a large annotated open dataset in the public domain. In response to this scenario, a new transfer learning method based on sub-domain adaptation (TLSDA), which involves a first sub-domain adaptation and then fine-tuning, was proposed in this study. Firstly, a modified deep sub-domain adaptation network with pseudo-label (DSAN-PL) was proposed to align the feature spaces of a public domain (labeled) and a private domain (unlabeled). The DSAN-PL model was then fine-tuned using a small amount of labeled OCT data from the private domain. We tested our method on three open OCT datasets, using one as the public domain and the other two as the private domains. Remarkably, with only 10% labeled OCT images (~100 images per category), TLSDA achieved classification accuracies of 93.63% and 96.59% on the two private datasets, significantly outperforming conventional transfer learning approaches. With the Gradient-weighted Class Activation Map (Grad-CAM) technique, it was observed that the proposed method could more precisely localize the subtle lesion regions for OCT image classification. TLSDA could be a potential technique for applications where only a small number of images is labeled in a private domain and there exists a public database having a large number of labeled images with domain difference.
Multidimensional Diffusion-Wave-Type Solutions to the Second-Order Evolutionary Equation
Authors: Kazakov Alexander , Lempert Anna
Published: January 2024

The paper concerns a nonlinear second-order parabolic evolution equation, one of the well-known objects of mathematical physics, which describes the processes of high-temperature thermal conductivity, nonlinear diffusion, filtration of liquid in a porous medium and some other processes in continuum mechanics. A particular case of it is the well-known porous medium equation. Unlike previous studies, we consider the case of several spatial variables. We construct and study solutions that describe disturbances propagating over a zero background with a finite speed, usually called ‘diffusion-wave-type solutions’. Such effects are atypical for parabolic equations and appear since the equation degenerates on manifolds where the desired function vanishes. The paper pays special attention to exact solutions of the required type, which can be expressed as either explicit or implicit formulas, as well as a reduction of the partial differential equation to an ordinary differential equation that cannot be integrated in quadratures. In this connection, Cauchy problems for second-order ordinary differential equations arise, inheriting the singularities of the original formulation. We prove the existence of continuously differentiable solutions for them. A new example, an analog of the classic example by S.V. Kovalevskaya for the considered case, is constructed. We also proved a new existence and uniqueness theorem of heat-wave-type solutions in the class of piece-wise analytic functions, generalizing previous ones. During the proof, we transit to the hodograph plane, which allows us to overcome the analytical difficulties.
Development of Mass–Energy Balance Model Based on a New Process of RSF with Hy-O-CR
Authors: Li Haifeng , Chen Jingran , Luo Zhiguo , Wang Xiaoai
Published: January 2024

At present, blast furnace (BF) ironmaking is still the main process for producing hot metal in China and around the world. Under the constraint of the global goal of “double carbon”, it is urgent to carry out hydrogen metallurgical innovation for the existing BF ironmaking process with higher carbon emissions. In recent years, BF technology with hydrogen enrichment and pure oxygen has made some progress, effectively reducing carbon emissions of hot metal per tons, but it is still unable to break through the technical bottleneck of emission reduction of more than 30%. In view of this, the authors put forward an ironmaking technology of a reduction smelting furnace (RSF) that is hydrogen-rich and utilizes pure oxygen and carbon recycle (Hy-O-CR), which breaks through the technical defect of traditional BF emission reduction of less than 30% by reshaping the furnace. Firstly, the construction process of the mass and energy balance model for two main unit modules in the new process (RSF with Hy-O-CR and top gas cycle) is introduced, and then the parameter optimization under specific scenario conditions is analyzed, and the influence mechanism of several key variables on the parameters in the furnace is obtained. Finally, the emission of CO2 in the whole process is explored in the case of two typical operating parameters. The results show that after using CCUS technology, the minimum value of direct CO2 emission is 215.93 kg/tHM, which is as high as 84.58% compared with the traditional BF process. Even if the removed CO2 is counted in carbon emissions, the minimum value of direct or indirect carbon emissions is 729.85 kg/tHM, and the proportion of emission reduction can reach 47.87%. The research results show that the reconstruction of Hy-O-CR technology can change the ratio of direct reduction and indirect reduction, which greatly breaks through the emission limit of the traditional BF and provides a new reference for hydrogen metallurgy technology and a basis for further study of the optimization of RSF size.
Research on the Tribological Properties of a New Generation of Multi-Layer Nanostructured PVD Coatings for Increasing the Technological Lifetime of Moulds
Authors: Brezinová Janette , Džupon Miroslav , Puchý Viktor , Brezina Jakub , Maruschak Pavlo , Guzanová Anna , Sobotová Lýdia , Badida Miroslav
Published: January 2024

This paper presents the results of research focused on increasing the lifespan of HPDC moulds for casting aluminium alloys by applying duplex PVD coatings in combination with laser texturing the base material before the coatings’ deposition. This article describes the HPDC process and the degradation mechanisms of the moulds that arose during this process. The PVD nanostructured coatings utilised, the methods of their deposition, and the evaluation of their wear resistance are defined in this paper. The surface texturing process is described alongside the description of the analysis of the wear of the functional parts of the mould after decommissioning, which was carried out by visual inspection and optical and light microscopy. Three types of PVD duplex coatings were analysed during our study. The coatings were deposited using the LARC technology method (lateral rotating cathode). Subsequently, the procedure of laser texturing in the form of dimple textures using a laser was proposed. The quality of the coatings was evaluated under tribological conditions by means of the “Ball on disc” method. Based on the experimental results, recommendations for practice are established.
Biomarkers of Metabolic Adaptation to High Dietary Fats in a Mouse Model of Obesity Resistance
Authors: Milhem Fadia , Hamilton Leah M. , Skates Emily , Wilson Mickey , Johanningsmeier Suzanne D. , Komarnytsky Slavko
Published: January 2024

Obesity-resistant (non-responder, NR) phenotypes that exhibit reduced susceptibility to developing obesity despite being exposed to high dietary fat are crucial in exploring the metabolic responses that protect against obesity. Although several efforts have been made to study them in mice and humans, the individual protective mechanisms are poorly understood. In this exploratory study, we used a polygenic C57BL/6J mouse model of diet-induced obesity to show that NR mice developed healthier fat/lean body mass ratios (0.43 ± 0.05) versus the obesity-prone (super-responder, SR) phenotypes (0.69 ± 0.07, p < 0.0001) by upregulating gene expression networks that promote the accumulation of type 2a, fast-twitch, oxidative muscle tissues. This was achieved in part by a metabolic adaptation in the form of blood glucose sparing, thus aggravating glucose tolerance. Resistance to obesity in NR mice was associated with 4.9-fold upregulated mitoferrin 1 (Slc25a37), an essential mitochondrial iron importer. SR mice also showed fecal volatile metabolite signatures of enhanced short-chain fatty acid metabolism, including increases in detrimental methyl formate and ethyl propionate, and these effects were reversed in NR mice. Continued research into obesity-resistant phenotypes can offer valuable insights into the underlying mechanisms of obesity and metabolic health, potentially leading to more personalized and effective approaches for managing weight and related health issues.
Analysis of the Fecal Metabolomic Profile in Breast vs. Different Formula Milk Feeding in Late Preterm Infants
Authors: De Bernardo Giuseppe , D’Urso Gilda , Spadarella Simona , Giordano Maurizio , Leone Giuseppina , Casapullo Agostino
Published: January 2024

Human milk is the gold standard for infant nutrition, but when it is not available or insufficient to satisfy the needs of the infant, formula milk is proposed as an effective substitute. A prospective observational cohort study was conducted on late preterm infants fed with breast and two different formula milks. On this basis, they were divided into three groups: group FMPB (fed with formula + postbiotic), group FM (fed with standard formula), and group BM (breastfed). Stool samples for a metabolomic study were collected at T0 (5–7 days after birth), T1 (30 days of life), and T2 (90 days of life), giving rise to 74 samples analyzed via liquid chromatography coupled with high-resolution mass spectrometry. The T0, T1, and T2 LC-MS raw data were processed for Partial Least Square Discriminant Analysis (PLS-DA), followed by a statistical analysis. This preliminary study highlighted a good overlapping between the fecal metabolome of breast and substitute feeding systems, confirming the efficacy of the formula preparations as breast milk substitutes. Moreover, several similarities were also detected between the FMPB and BM metabolome, highlighting that the addition of a postbiotic to standard formula milk could be more effective and considered a better alternative to breast milk.
Gut Microbiota–Gut Metabolites and Clostridioides difficile Infection: Approaching Sustainable Solutions for Therapy
Authors: Gurung Bijay , Stricklin Maranda , Wang Shaohua
Published: January 2024

Clostridioides difficile (C. difficile) infection (CDI) is the most common hospital-acquired infection. With the combination of a high rate of antibiotic resistance and recurrence, it has proven to be a debilitating public health threat. Current treatments for CDI include antibiotics and fecal microbiota transplantation, which contribute to recurrent CDIs and potential risks. Therefore, there is an ongoing need to develop new preventative treatment strategies for CDI. Notably, gut microbiota dysbiosis is the primary risk factor for CDI and provides a promising target for developing novel CDI therapy approaches. Along with gut microbiota dysbiosis, a reduction in important gut metabolites like secondary bile acids and short-chain fatty acids (SCFAs) were also seen in patients suffering from CDI. In this review study, we investigated the roles and mechanisms of gut microbiota and gut microbiota-derived gut metabolites, especially secondary bile acids and SCFAs in CDI pathogenesis. Moreover, specific signatures of gut microbiota and gut metabolites, as well as different factors that can modulate the gut microbiota, were also discussed, indicating that gut microbiota modulators like probiotics and prebiotics can be a potential therapeutic strategy for CDI as they can help restore gut microbiota and produce gut metabolites necessary for a healthy gut. The understanding of the associations between gut microbiota–gut metabolites and CDI will allow for developing precise and sustainable approaches, distinct from antibiotics and fecal transplant, for mitigating CDI and other gut microbiota dysbiosis-related diseases.
Reliability of Time-Series Plasma Metabolome Data over 6 Years in a Large-Scale Cohort Study
Authors: Miyake Atsuko , Harada Sei , Sugiyama Daisuke , Matsumoto Minako , Hirata Aya , Miyagawa Naoko , Toki Ryota , Edagawa Shun , Kuwabara Kazuyo , Okamura Tomonori , Sato Asako , Amano Kaori , Hirayama Akiyoshi , Sugimoto Masahiro , Soga Tomoyoshi , Tomita Masaru , Arakawa Kazuharu , Takebayashi Toru , Iida Miho
Published: January 2024

Studies examining long-term longitudinal metabolomic data and their reliability in large-scale populations are limited. Therefore, we aimed to evaluate the reliability of repeated measurements of plasma metabolites in a prospective cohort setting and to explore intra-individual concentration changes at three time points over a 6-year period. The study participants included 2999 individuals (1317 men and 1682 women) from the Tsuruoka Metabolomics Cohort Study, who participated in all three surveys—at baseline, 3 years, and 6 years. In each survey, 94 plasma metabolites were quantified for each individual and quality control (QC) sample. The coefficients of variation of QC, intraclass correlation coefficients, and change rates of QC were calculated for each metabolite, and their reliability was classified into three categories: excellent, fair to good, and poor. Seventy-six percent (71/94) of metabolites were classified as fair to good or better. Of the 39 metabolites grouped as excellent, 29 (74%) in men and 26 (67%) in women showed significant intra-individual changes over 6 years. Overall, our study demonstrated a high degree of reliability for repeated metabolome measurements. Many highly reliable metabolites showed significant changes over the 6-year period, suggesting that repeated longitudinal metabolome measurements are useful for epidemiological studies.
Split-Gate: Harnessing Gate Modulation Power in Thin-Film Electronics
Authors: Lee Subin , Kim Yeong Jae , Yoo Hocheon
Published: January 2024

With the increase in electronic devices across various applications, there is rising demand for selective carrier control. The split-gate consists of a gate electrode divided into multiple parts, allowing for the independent biasing of electric fields within the device. This configuration enables the potential formation of both p- and n-channels by injecting holes and electrons owing to the presence of the two gate electrodes. Applying voltage to the split-gate allows for the control of the Fermi level and, consequently, the barrier height in the device. This facilitates band bending in unipolar transistors and allows ambipolar transistors to operate as if unipolar. Moreover, the split-gate serves as a revolutionary tool to modulate the contact resistance by controlling the barrier height. This approach enables the precise control of the device by biasing the partial electric field without limitations on materials, making it adaptable for various applications, as reported in various types of research. However, the gap length between gates can affect the injection of the electric field for the precise control of carriers. Hence, the design of the gap length is a critical element for the split-gate structure. The primary investigation in this review is the introduction of split-gate technology applied in various applications by using diverse materials, the methods for forming the split-gate in each device, and the operational mechanisms under applied voltage conditions.
Highlighting New Perspectives on Musculoskeletal Infections
Authors: Fiore Michele , Sambri Andrea
Published: January 2024

The treatment of musculoskeletal and prosthetic joint infections represents a considerable challenge for patients, healthcare providers, and the healthcare system because of the high number of treatment failures and the significant economic burden [...]
Metagenomic Characterisation of the Gut Microbiome and Effect of Complementary Feeding on Bifidobacterium spp. in Australian Infants
Authors: Parkin Kimberley , Palmer Debra J. , Verhasselt Valerie , Amenyogbe Nelly , Cooper Matthew N. , Christophersen Claus T. , Prescott Susan L. , Silva Desiree , Martino David
Published: January 2024

Complementary feeding induces dramatic ecological shifts in the infant gut microbiota toward more diverse compositions and functional metabolic capacities, with potential implications for immune and metabolic health. The aim of this study was to examine whether the age at which solid foods are introduced differentially affects the microbiota in predominantly breastfed infants compared with predominantly formula-fed infants. We performed whole-genome shotgun metagenomic sequencing of infant stool samples from a cohort of six-month-old Australian infants enrolled in a nested study within the ORIGINS Project longitudinal birth cohort. Infants born preterm or those who had been administered antibiotics since birth were excluded. The taxonomic composition was highly variable among individuals at this age. Predominantly formula-fed infants exhibited a higher microbiome diversity than predominantly breastfed infants. Among the predominantly breastfed infants, the introduction of solid foods prior to five months of age was associated with higher alpha diversity than solid food introduction after six months of age, primarily due to the loss of Bifidobacterium infantis. In contrast, the age at which solid food was introduced was not associated with the overall change in diversity among predominantly formula-fed infants but was associated with compositional changes in Escherichia abundance. Examining the functional capacity of the microbiota in relation to these changes, we found that the introduction of solid foods after six months of age was associated with elevated one-carbon compound metabolic pathways in both breastfed and formula-fed infants, although the specific metabolic sub-pathways differed, likely reflecting different taxonomic compositions. Our findings suggest that the age of commencement of solid foods influences the gut microbiota composition differently in predominantly breastfed infants than in predominantly formula-fed infants.
Asymmetric Schottky Barrier-Generated MoS2/WTe2 FET Biosensor Based on a Rectified Signal
Authors: Zhang Xinhao , Chen Shuo , Ma Heqi , Sun Tianyu , Cui Xiangyong , Huo Panpan , Man Baoyuan , Yang Cheng
Published: January 2024

Field-effect transistor (FET) biosensors can be used to measure the charge information carried by biomolecules. However, insurmountable hysteresis in the long-term and large-range transfer characteristic curve exists and affects the measurements. Noise signal, caused by the interference coefficient of external factors, may destroy the quantitative analysis of trace targets in complex biological systems. In this report, a “rectified signal” in the output characteristic curve, instead of the “absolute value signal” in the transfer characteristic curve, is obtained and analyzed to solve these problems. The proposed asymmetric Schottky barrier-generated MoS2/WTe2 FET biosensor achieved a 105 rectified signal, sufficient reliability and stability (maintained for 60 days), ultra-sensitive detection (10 aM) of the Down syndrome-related DYRK1A gene, and excellent specificity in base recognition. This biosensor with a response range of 10 aM–100 pM has significant application potential in the screening and rapid diagnosis of Down syndrome.
A High-Performance Strain Sensor for the Detection of Human Motion and Subtle Strain Based on Liquid Metal Microwire
Authors: Zhu He , Sun Zheng , Wang Xin , Xia Hong
Published: January 2024

Flexible strain sensors have a wide range of applications, such as human motion monitoring, wearable electronic devices, and human–computer interactions, due to their good conformability and sensitive deformation detection. To overcome the internal stress problem of solid sensing materials during deformation and prepare small-sized flexible strain sensors, it is necessary to choose a more suitable sensing material and preparation technology. We report a simple and high-performance flexible strain sensor based on liquid metal nanoparticles (LMNPs) on a polyimide substrate. The LMNPs were assembled using the femtosecond laser direct writing technology to form liquid metal microwires. A wearable strain sensor from the liquid metal microwire was fabricated with an excellent gauge factor of up to 76.18, a good linearity in a wide sensing range, and a fast response/recovery time of 159 ms/120 ms. Due to these extraordinary strain sensing performances, the strain sensor can monitor facial expressions in real time and detect vocal cord vibrations for speech recognition.
Layer-Dependent Sensing Performance of WS2-Based Gas Sensors
Authors: Zhou You , Wang Sheng , Xin Sichen , Sayin Sezin , Yi Zhiqiang , Li Zhenyu , Zaghloul Mona
Published: January 2024

Two-dimensional (2D) materials, such as tungsten disulfide (WS2), have attracted considerable attention for their potential in gas sensing applications, primarily due to their distinctive electrical properties and layer-dependent characteristics. This research explores the impact of the number of WS2 layers on the ability to detect gases by examining the layer-dependent sensing performance of WS2-based gas sensors. We fabricated gas sensors based on WS2 in both monolayer and multilayer configurations and methodically evaluated their response to various gases, including NO2, CO, NH3, and CH4 at room temperature and 50 degrees Celsius. In contrast to the monolayer counterpart, the multilayer WS2 sensor exhibits enhanced gas sensing performance at higher temperatures. Furthermore, a comprehensive gas monitoring system was constructed employing these WS2-based sensors, integrated with additional electronic components. To facilitate user access to data and receive alerts, sensor data were transmitted to a cloud-based platform for processing and storage. This investigation not only advances our understanding of 2D WS2-based gas sensors but also underscores the importance of layer engineering in tailoring their sensing capabilities for diverse applications. Additionally, the development of a gas monitoring system employing 2D WS2 within this study holds significant promise for future implementation in intelligent, efficient, and cost-effective sensor technologies.
Impact of Plasmodium relictum Infection on the Colonization Resistance of Bird Gut Microbiota: A Preliminary Study
Authors: Aželytė Justė , Maitre Apolline , Abuin-Denis Lianet , Piloto-Sardiñas Elianne , Wu-Chuang Alejandra , Žiegytė Rita , Mateos-Hernández Lourdes , Obregón Dasiel , Cabezas-Cruz Alejandro , Palinauskas Vaidas
Published: January 2024

Avian malaria infection has been known to affect host microbiota, but the impact of Plasmodium infection on the colonization resistance in bird gut microbiota remains unexplored. This study investigated the dynamics of Plasmodium relictum infection in canaries, aiming to explore the hypothesis that microbiota modulation by P. relictum would reduce colonization resistance. Canaries were infected with P. relictum, while a control group was maintained. The results revealed the presence of P. relictum in the blood of all infected canaries. Analysis of the host microbiota showed no significant differences in alpha diversity metrics between infected and control groups. However, significant differences in beta diversity indicated alterations in the microbial taxa composition of infected birds. Differential abundance analysis identified specific taxa with varying prevalence between infected and control groups at different time points. Network analysis demonstrated a decrease in correlations and revealed that P. relictum infection compromised the bird microbiota’s ability to resist the removal of taxa but did not affect network robustness with the addition of new nodes. These findings suggest that P. relictum infection reduces gut microbiota stability and has an impact on colonization resistance. Understanding these interactions is crucial for developing strategies to enhance colonization resistance and maintain host health in the face of parasitic infections.
Sex and Cross-Sex Testosterone Treatment Alters Gamma-Hydroxybutyrate Acid Toxicokinetics and Toxicodynamics in Rats
Authors: Zhang Qing , Wei Hao , Lee Annie , Felmlee Melanie A.
Published: January 2024

Γ-hydroxybutyric acid (GHB) is widely abused due to its sedative/hypnotic and euphoric effects. In recent years, GHB use has witnessed a notable rise within the LGBTQ+ community. GHB is a substrate of monocarboxylate transporters (MCTs) and exhibits nonlinear toxicokinetics, characterized by saturable metabolism, absorption, and renal reabsorption. This study investigates the impact of exogenous testosterone administration on GHB toxicokinetics and toxicodynamics, exploring the potential of MCT1 inhibition as a strategy to counteract toxicity. Ovariectomized (OVX) females and castrated (CST) male Sprague Dawley rats were treated with testosterone or placebo for 21 days. GHB was administered at two doses (1000 mg/kg or 1500 mg/kg i.v.), and the MCT1 inhibitor AR-C 155858 (1 mg/kg i.v.) was administered 5 min after GHB (1500 mg/kg i.v.) administration. Plasma and urine were collected up to 8 h post-dose, and GHB concentrations were quantified via a validated LC/MS/MS assay. Sleep time (sedative/hypnotic effect) was utilized as the toxicodynamic endpoint. Testosterone treatment significantly affected GHB toxicokinetics and toxicodynamics. Testosterone-treated CST rats exhibited significantly lower renal clearance, higher AUC, and increased sedative effect, while testosterone-treated OVX rats demonstrated higher metabolic clearance. AR-C 155858 treatment led to an increase in GHB renal and total clearance together with an improvement in sedative/hypnotic effect. In conclusion, exogenous testosterone treatment induces significant alterations in GHB toxicokinetics and toxicodynamics, and MCT inhibition can serve as a potential therapeutic strategy for GHB overdose in both cisgender and transgender male populations.
Drug Exposure and Susceptibility of Pyrazinamide Correlate with Treatment Response in Pyrazinamide-Susceptible Patients with Multidrug-Resistant Tuberculosis
Authors: Dong Shulan , Shao Ge , Davies Forsman Lina , Wang Sainan , Wang Shanshan , Cao Jiayi , Bao Ziwei , Bruchfeld Judith , Alffenaar Jan-Willem C. , Liu Jia , Hu Yi , Wu Meiying
Published: January 2024

Exploring the influence of pyrazinamide exposure and susceptibility on treatment response is crucial for optimizing the management of multidrug-resistant tuberculosis (MDR-TB). This study aimed to investigate the association between pyrazinamide exposure, susceptibility, and response to MDR-TB treatment, as well as find clinical thresholds for pyrazinamide. A prospective multi-center cohort study of participants with MDR-TB using pyrazinamide was conducted in three TB-designated hospitals in China. Univariate and multivariate analyses were applied to investigate the associations. Classification and Regression Tree (CART) analysis was used to identify clinical thresholds, which were further evaluated by multivariate analysis and receiver operating characteristic (ROC) curves. The study included 143 patients with MDR-TB. The exposure/susceptibility ratio of pyrazinamide was associated with two-month culture conversion (adjusted risk ratio (aRR), 1.1; 95% confidence interval (CI), 1.07–1.20), six-month culture conversion (aRR, 1.1; 95% CI, 1.06–1.16), treatment success (aRR, 1.07; 95% CI, 1.03–1.10), as well as culture conversion time (adjusted hazard ratio (aHR) 1.18; 95% CI,1.14–1.23). The threshold for optimal improvement in sputum culture results at the sixth month of treatment was determined to be a pyrazinamide AUC0–24h/MIC ratio of 7.8. In conclusion, the exposure/susceptibility ratio of pyrazinamide is associated with the treatment response of MDR-TB, which may change in different Group A drug-based regimens.
Off-Label Immunosuppressant Drugs in Solid Organ Transplantation
Authors: Nogueiras-Álvarez Rita , García-Sáiz María del Mar
Published: January 2024

Once a solid organ transplantation (SOT) has been performed, it is necessary to prescribe immunosuppressant medication to prevent graft rejection. This task has the peculiarity that many of these drugs do not have specific indications for transplant use in the technical data sheets. We performed a review of different immunosuppressive drugs’ information available at European and American regulatory agencies in order to analyze the approved indications by the type of SOT. In our work, besides showing these differences between different indication approvals in different SOT modalities, we also attempted to reflect other differences under the approved indications according to age group, formulation type, geographical area, etc. Although consensus documents on the subject have been published, the access to immunosuppressants depends on each country’s regulation and healthcare system, and off-label prescription is a reality that healthcare professionals need to be familiar with.