bluuebunny/crossref_metadata_2025 huggingface dataset:
An Efficient Memetic Algorithm for the Minimum Load Coloring Problem
Authors: Zhang Zhiqiang , Li Zhongwen , Qiao Xiaobing , Wang Weijun
Published: May 2019

Given a graph G with n vertices and l edges, the load distribution of a coloring q: V → {red, blue} is defined as dq = (rq, bq), in which rq is the number of edges with at least one end-vertex colored red and bq is the number of edges with at least one end-vertex colored blue. The minimum load coloring problem (MLCP) is to find a coloring q such that the maximum load, lq = 1/l × max{rq, bq}, is minimized. This problem has been proved to be NP-complete. This paper proposes a memetic algorithm for MLCP based on an improved K-OPT local search and an evolutionary operation. Furthermore, a data splitting operation is executed to expand the data amount of global search, and a disturbance operation is employed to improve the search ability of the algorithm. Experiments are carried out on the benchmark DIMACS to compare the searching results from memetic algorithm and the proposed algorithms. The experimental results show that a greater number of best results for the graphs can be found by the memetic algorithm, which can improve the best known results of MLCP.
A Mizoguchi–Takahashi Type Fixed Point Theorem in Complete Extended b-Metric Spaces
Authors: Alamgir Nayab , Kiran Quanita , Aydi Hassen , Mukheimer Aiman
Published: May 2019

In this paper, we prove a new fixed point theorem for a multi-valued mapping from a complete extended b-metric space U into the non empty closed and bounded subsets of U, which generalizes Nadler’s fixed point theorem. We also establish some fixed point results, which generalize our first result. Furthermore, we establish Mizoguchi–Takahashi’s type fixed point theorem for a multi-valued mapping from a complete extended b-metric space U into the non empty closed and bounded subsets of U that improves many existing results in the literature.
Generalized (σ,ξ)-Contractions and Related Fixed Point Results in a P.M.S
Authors: Parvaneh Vahid , Mohammadi Babak , Aydi Hassen , Mukheimer Aiman
Published: May 2019

In this paper, we present the concept of Θ − ( σ , ξ ) Ω -contraction mappings and we nominate some related fixed point results in ordered p-metric spaces. Our results extend several famous ones in the literature. Some examples and an application are given in order to validate our results.
The Gegenbauer Wavelets-Based Computational Methods for the Coupled System of Burgers’ Equations with Time-Fractional Derivative
Authors: Ozdemir Neslihan , Secer Aydin , Bayram Mustafa
Published: May 2019

In this study, Gegenbauer wavelets are used to present two numerical methods for solving the coupled system of Burgers’ equations with a time-fractional derivative. In the presented methods, we combined the operational matrix of fractional integration with the Galerkin method and the collocation method to obtain a numerical solution of the coupled system of Burgers’ equations with a time-fractional derivative. The properties of Gegenbauer wavelets were used to transform this system to a system of nonlinear algebraic equations in the unknown expansion coefficients. The Galerkin method and collocation method were used to find these coefficients. The main aim of this study was to indicate that the Gegenbauer wavelets-based methods is suitable and efficient for the coupled system of Burgers’ equations with time-fractional derivative. The obtained results show the applicability and efficiency of the presented Gegenbaur wavelets-based methods.
Anti-Obesity Effect of Standardized Extract of Microalga Phaeodactylum tricornutum Containing Fucoxanthin
Authors: Koo Song Yi , Hwang Ji-Hyun , Yang Seung-Hoon , Um Jae-In , Hong Kwang Won , Kang Kyungsu , Pan Cheol-Ho , Hwang Keum Taek , Kim Sang Min
Published: May 2019

Fucoxanthin (FX), a marine carotenoid found in macroalgae and microalgae, exhibits several beneficial effects to health. The anti-obesity activity of FX is well documented, but FX has not been mass-produced or applied extensively or commercially because of limited availability of raw materials and complex extraction techniques. In this study, we investigated the anti-obesity effect of standardized FX powder (Phaeodactylum extract (PE)) developed from microalga Phaeodactylum tricornutum as a commercial functional food. The effects of PE on adipogenesis inhibition in 3T3-L1 adipocytes and anti-obesity in high-fat diet (HFD)-fed C57BL/6J mice were evaluated. PE and FX dose-dependently decreased intracellular lipid contents in adipocytes without cytotoxicity. In HFD-fed obese mice, PE supplementation for six weeks decreased body weight, organ weight, and adipocyte size. In the serum parameter analysis, the PE-treated groups showed attenuation of lipid metabolism dysfunction and liver damage induced by HFD. In the liver, uncoupling protein-1 (UCP1) upregulation and peroxisome proliferator activated receptor γ (PPARγ) downregulation were detected in the PE-treated groups. Additionally, micro computed tomography revealed lower fat accumulation in PE-treated groups compared to that in the HFD group. These results indicate that PE exerts anti-obesity effects by inhibiting adipocytic lipogenesis, inducing fat mass reduction and decreasing intracellular lipid content, adipocyte size, and adipose weight.
Physical Activity and Depressive Disorders in Pregnant Women—A Systematic Review
Authors: Kołomańska Daria , Zarawski Marcin , Mazur-Bialy Agnieszka
Published: May 2019

Background and Objectives: Pregnancy is a unique period in the life of every woman. The lifestyle of a pregnant woman has a significant impact on her and her child’s health. Regular physical activity is one of the elements that help maintain normal mental and physical well-being. In pregnant women who regularly have moderate physical activity, there is a lower risk of developing obesity and overweight. Physical exercises have an impact on maintaining proper muscular tonus, reduce pain and prepare for the exertion during labour. Based on the available literature, the aim of this study was to present the impact of physical activity on depressive disorders in pregnant women. Materials and Methods: A review of the literature was carried out in the Medline PubMed database. The basic search terms were: “pregnancy” AND “physical activity AND depression”. The work included only English-language publications published in the years 2000–2018. Results: A total of 408 references were found. On the basis of an analysis of titles, abstracts and the language of publication (other than English), 354 articles were rejected, and 54 articles were fully read, of which five were rejected due to lack of access to the full version. Finally, 17 references were included in the review. Conclusions: Physical activity, at least once a week, significantly reduces the symptoms of depression in pregnant women and may be an important factor in the prevention of depression in this period.
Disturbed Interstitial Pregnancy: A First Case of Successful Treatment Using a Mini-Laparoscopic Approach
Authors: Tantchev Latchesar , Kotzev Andrey , Yordanov Angel
Published: May 2019

Interstitial ectopic pregnancy (EP) is a life-threatening condition due to the risk of massive hemorrhage in the event of its disturbance. We present the case of a 27-year-old patient who was admitted with massive hemoperitoneum, caused by the rupture of an interstitial pregnancy in the area of the fallopian tube stump, which had been removed after a previous ectopic pregnancy. The condition was overcome using a mini-laparoscopic approach (2.6 mm, 30° optics), with one 3 mm port for micro-laparoscopic instruments and one 10 mm port. Such an approach has not yet been reported in the available literature, among the casuistically reported cases of pregnancy in the tubal stump. We consider that the technique is safe, completely in the interest of the patient, applicable by an experienced team, and in agreement with modern trends regarding the minimization of operative access.
Food Protein-Induced Enterocolitis Syndrome: Proposals for New Definitions
Authors: Miceli Sopo Stefano , Gelsomino Mariannita , Rivetti Serena , Del Vescovo Ester
Published: May 2019

Acute food protein-induced enterocolitis syndrome (FPIES) is a non-IgE-mediated allergy and is characterized by repetitive profuse vomiting episodes, often in association with pallor, lethargy, and diarrhea, presenting within 1–4 h from the ingestion of a triggering food. In 2017, the international consensus guidelines for the diagnosis and management of FPIES were published. They cover all aspects of this syndrome, which in recent decades has attracted the attention of pediatric allergists. In particular, the consensus proposed innovative diagnostic criteria. However, the diagnosis of acute FPIES is still currently discussed because the interest in this disease is relatively recent and, above all, there are no validated panels of diagnostic criteria. We propose some ideas for reflection on the diagnostic and suspicion criteria of acute FPIES with exemplary stories of children certainly or probably suffering from acute FPIES. For example, we believe that new definitions should be produced for mild forms of FPIES, multiple forms, and those with IgE-mediated symptoms. Moreover, we propose two clinical criteria to suspect acute FPIES and to refer the child to the diagnostic oral food challenge.
Non-Celiac Gluten Sensitivity: A Review
Authors: Roszkowska Anna , Pawlicka Marta , Mroczek Anna , Bałabuszek Kamil , Nieradko-Iwanicka Barbara
Published: May 2019

Background and objectives: Grain food consumption is a trigger of gluten related disorders: celiac disease, non-celiac gluten sensitivity (NCGS) and wheat allergy. They demonstrate with non-specific symptoms: bloating, abdominal discomfort, diarrhea and flatulence. Aim: The aim of the review is to summarize data about pathogenesis, symptoms and criteria of NCGS, which can be helpful for physicians. Materials and Methods: The PubMed and Google Scholar databases were searched in January 2019 with phrases: ’non-celiac gluten sensitivity’, non-celiac gluten sensitivity’, non-celiac wheat gluten sensitivity’, non-celiac wheat gluten sensitivity’, and gluten sensitivity’. More than 1000 results were found. A total of 67 clinical trials published between 1989 and 2019 was scanned. After skimming abstracts, 66 articles were chosen for this review; including 26 clinical trials. Results: In 2015, Salerno Experts’ Criteria of NCGS were published. The Salerno first step is assessing the clinical response to gluten free diet (GFD) and second is measuring the effect of reintroducing gluten after a period of treatment with GFD. Several clinical trials were based on the criteria. Conclusions: Symptoms of NCGS are similar to other gluten-related diseases, irritable bowel syndrome and Crohn’s disease. With Salerno Experts’ Criteria of NCGS, it is possible to diagnose patients properly and give them advice about nutritional treatment.
Radar Detection-Based Modeling in a Blast Furnace: A Prediction Model of Burden Surface Descent Speed
Authors: Tian Jiuzhou , Tanaka Akira , Hou Qingwen , Chen Xianzhong
Published: May 2019

The distribution of burden layers is a vital factor that affects the production of a blast furnace. Radars are advanced instruments that can provide the detection results of the burden surface shape inside a blast furnace in real time. To better estimate the burden layer thicknesses through improving the prediction accuracy of the burden descent during charging periods, an innovative data-driven model for predicting the distribution of the burden surface descent speed is proposed. The data adopted were from the detection results of an operating blast furnace, collected using a mechanical swing radar system. Under a kinematic continuum modeling mechanism, the proposed model adopts a linear combination of Gaussian radial basis functions to approximate the equivalent field of burden descent speed along the burden surface radius. A proof of the existence and uniqueness of the prediction solution is given to guarantee that the predicted radial profile of the burden surface can always be calculated numerically. Compared with the plain data-driven descriptive model, the proposed model has the ability to better characterize the variability in the radial distribution of burden descent speed. In addition, the proposed model provides prediction results of higher accuracy for both the future surface shape and descent speed distribution.
MetPC: Metabolite Pipeline Consisting of Metabolite Identification and Biomarker Discovery Under the Control of Two-Dimensional FDR
Authors: Kim Jaehwi , Jeong Jaesik
Published: May 2019

Due to the complex features of metabolomics data, the development of a unified platform, which covers preprocessing steps to data analysis, has been in high demand over the last few decades. Thus, we developed a new bioinformatics tool that includes a few of preprocessing steps and biomarker discovery procedure. For metabolite identification, we considered a hierarchical statistical model coupled with an Expectation–Maximization (EM) algorithm to take care of latent variables. For biomarker metabolite discovery, our procedure controls two-dimensional false discovery rate (fdr2d) when testing for multiple hypotheses simultaneously.
Polyphasic Characterization of Yeasts and Lactic Acid Bacteria Metabolic Contribution in Semi-Solid Fermentation of Chinese Baijiu (Traditional Fermented Alcoholic Drink): Towards the Design of a Tailored Starter Culture
Authors: Ma Rufei , Sui Lu , Zhang Jingsheng , Hu Jinrong , Liu Ping
Published: May 2019

Chinese Baijiu is principally produced through a spontaneous fermentation process, which involves complex microorganism communities. Among them, yeasts and lactic acid bacteria (LAB) are important communities. The study examined the isolated strains from fermented grains of Baijiu regarding their activity of α-amylase and glucoamylase, ethanol tolerance, glucose utilization, as well as metabolite production in the process of laboratory-scale sorghum-based fermentation. Selected strains (Saccharomycopsis fibuligera 12, Saccharomyces cerevisiae 3, and Pediococcus acidilactici 4) were blended in different combinations. The influence of selected strains on the metabolic variation in different semi-solid fermentations was investigated by gas chromatography–mass spectrometry (GC–MS) accompanied by multivariate statistical analysis. According to the principal component analysis (PCA), the metabolites produced varied in different mixtures of pure cultures. S. fibuligera produced various enzymes, particularly α-amylase and glucoamylase, and exhibited a better performance compared with other species regarding the ability to convert starch to soluble sugars and positively affect the production process of volatile compounds. S. cerevisiae had a high fermentation capacity, thereby contributing to substrates utilization. Lactic acid bacteria had a good ability to produce lactic acid. This study attaches importance to the special functions of S. fibuligera, S. cerevisiae, and P. acidilactici in Chinese Baijiu making, and investigates their metabolic characteristics in the process of lab-scale semi-solid fermentation.
Metabolite Profiling: A Tool for the Biochemical Characterisation of Mycobacterium sp.
Authors: Drapal Margit , Fraser Paul D.
Published: May 2019

Over the last decades, the prevalence of drug-resistance in Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis, has increased. These findings have rekindled interest in elucidating the unique adaptive molecular and biochemistry physiology of Mycobacterium. The use of metabolite profiling independently or in combination with other levels of “-omic” analyses has proven an effective approach to elucidate key physiological/biochemical mechanisms associated with Mtb throughout infection. The following review discusses the use of metabolite profiling in the study of tuberculosis, future approaches, and the technical and logistical limitations of the methodology.
The Chlamydia trachomatis Extrusion Exit Mechanism Is Regulated by Host Abscission Proteins
Authors: Zuck Meghan , Hybiske Kevin
Published: May 2019

The cellular exit strategies of intracellular pathogens have a direct impact on microbial dissemination, transmission, and engagement of immune responses of the host. Chlamydia exit their host via a budding mechanism called extrusion, which offers protective benefits to Chlamydia as they navigate their extracellular environment. Many intracellular pathogens co-opt cellular abscission machinery to facilitate cell exit, which is utilized to perform scission of two newly formed daughter cells following mitosis. Similar to viral budding exit strategies, we hypothesize that an abscission-like mechanism is required to physically sever the chlamydial extrusion from the host cell, co-opting the membrane fission activities of the endosomal sorting complex required for transport (ESCRT) family of proteins that are necessary for cellular scission events, including abscission. To test this, C. trachomatis L2-infected HeLa cells were depleted of key abscission machinery proteins charged multivesicle body protein 4b (CHMP4B), ALIX, centrosome protein 55 (CEP55), or vacuolar protein sorting-associated protein 4A (VPS4A), using RNA interference (RNAi). Over 50% reduction in extrusion formation was achieved by depletion of CHMP4B, VPS4A, and ALIX, but no effect on extrusion was observed with CEP55 depletion. These results demonstrate a role for abscission machinery in C. trachomatis extrusion from the host cell, with ALIX, VPS4A and CHMP4B playing key functional roles in optimal extrusion release.
Inactivation of Escherichia coli O157:H7 by High Hydrostatic Pressure Combined with Gas Packaging
Authors: Zhou Bing , Zhang Luyao , Wang Xiao , Dong Peng , Hu Xiaosong , Zhang Yan
Published: May 2019

The inactivation of Escherichia coli O157:H7 (E. coli) in physiological saline and lotus roots by high hydrostatic pressure (HHP) in combination with CO2 or N2 was studied. Changes in the morphology, cellular structure, and membrane permeability of the cells in physiological saline after treatments were investigated using scanning electron microscopy, transmission electron microscopy, and flow cytometry, respectively. It was shown that after HHP treatments at 150–550 MPa, CO2-packed E. coli cells had higher inactivation than the N2-packed and vacuum-packed cells, and no significant difference was observed in the latter two groups. Further, both the morphology and intracellular structure of CO2-packed E.coli cells were strongly destroyed by high hydrostatic pressure. However, serious damage to the intracellular structures occurred in only the N2-packed E. coli cells. During HHP treatments, the presence of CO2 caused more disruptions in the membrane of E. coli cells than in the N2-packed and vacuum-packed cells. These results indicate that the combined treatment of HHP and CO2 had a strong synergistic bactericidal effect, whereas N2 did not have synergistic effects with HHP. Although these two combined treatments had different effects on the inactivation of E. coli cells, the inactivation mechanisms might be similar. During both treatments, E. coli cells were inactivated by cell damage induced to the cellular structure through the membrane components and the extracellular morphology, unlike the independent HHP treatment.
Simultaneous Biological and Chemical Removal of Sulfate and Fe(II)EDTA-NO in Anaerobic Conditions and Regulation of Sulfate Reduction Products
Authors: Zhang Yu , Sun Lijian , Zhou Jiti
Published: May 2019

In the simultaneous flue gas desulfurization and denitrification by biological combined with chelating absorption technology, SO2 and NO are converted into sulfate and Fe(II)EDTA-NO which need to be reduced in biological reactor. Increasing the removal loads of sulfate and Fe(II)EDTA-NO and converting sulfate to elemental sulfur will benefit the application of this process. A moving-bed biofilm reactor was adopted for sulfate and Fe(II)EDTA-NO biological reduction. The removal efficiencies of the sulfate and Fe(II)EDTA-NO were 96% and 92% with the influent loads of 2.88 kg SO42−·m−3·d−1 and 0.48 kg NO·m−3·d−1. The sulfide produced by sulfate reduction could be reduced by increasing the concentrations of Fe(II)EDTA-NO and Fe(III)EDTA. The main reduction products of sulfate and Fe(II)EDTA-NO were elemental sulfur and N2. It was found that the dominant strain of sulfate reducing bacteria in the system was Desulfomicrobium. Pseudomonas, Sulfurovum and Arcobacter were involved in the reduction of Fe(II)EDTA-NO.
The Up-Regulation of Oxidative Stress as a Potential Mechanism of Novel MAO-B Inhibitors for Glioblastoma Treatment
Authors: Marconi Guya Diletta , Gallorini Marialucia , Carradori Simone , Guglielmi Paolo , Cataldi Amelia , Zara Susi
Published: May 2019

Gliomas are malignant brain tumors characterized by rapid spread and growth into neighboring tissues and graded I–IV by the World Health Organization. Glioblastoma is the fastest growing and most devastating IV glioma. The aim of this paper is to evaluate the biological effects of two potent and selective Monoamine Oxidase B (MAO-B) inhibitors, Cmp3 and Cmp5, in C6 glioma cells and in CTX/TNA2 astrocytes in terms of cell proliferation, apoptosis occurrence, inflammatory events and cell migration. These compounds decrease C6 glioma cells viability sparing normal astrocytes. Cell cycle analysis, the Mitochondrial Membrane Potential (MMP) and Reactive Oxygen Species (ROS) production were detected, revealing that Cmp3 and Cmp5 induce a G1 or G2/M cell cycle arrest, as well as a MMP depolarization and an overproduction of ROS; moreover, they inhibit the expression level of inducible nitric oxide synthase 2, thus contributing to fatal drug-induced oxidative stress. Cmp5 notably reduces glioma cell migration via down-regulating Matrix Metalloproteinases 2 and 9. This study demonstrated that our novel MAO-B inhibitors increase the oxidative stress level resulting in a cell cycle arrest and markedly reduces glioma cells migration thus reinforcing the hypothesis of a critical role-played by MAO-B in mediating oncogenesis in high-grade gliomas.
A Survey on Psycho-Physiological Analysis & Measurement Methods in Multimodal Systems
Authors: Baig Muhammad Zeeshan , Kavakli Manolya
Published: May 2019

Psycho-physiological analysis has gained greater attention in the last few decades in various fields including multimodal systems. Researchers use psychophysiological feedback devices such as skin conductance (SC), Electroencephalography (EEG) and Electrocardiography (ECG) to detect the affective states of the users during task performance. Psycho-physiological feedback has been successful in detection of the cognitive states of users in human-computer interaction (HCI). Recently, in game studies, psycho-physiological feedback has been used to capture the user experience and the effect of interaction on human psychology. This paper reviews several psycho-physiological, cognitive, and affective assessment studies and focuses on the use of psychophysiological signals in estimating the user’s cognitive and emotional states in multimodal systems. In this paper, we review the measurement techniques and methods that have been used to record psycho-physiological signals as well as the cognitive and emotional states in a variety of conditions. The aim of this review is to conduct a detailed study to identify, describe and analyze the key psycho-physiological parameters that relate to different mental and emotional states in order to provide an insight into key approaches. Furthermore, the advantages and limitations of these approaches are also highlighted in this paper. The findings state that the classification accuracy of >90% has been achieved in classifying emotions with EEG signals. A strong correlation between self-reported data, HCI experience, and psychophysiological data has been observed in a wide range of domains including games, human-robot interaction, mobile interaction, and simulations. An increase in β and γ -band activity have been observed in high intense games and simulations.
In vivo Efficacy and Safety Evaluation of Lactosyl-β-cyclodextrin as a Therapeutic Agent for Hepatomegaly in Niemann-Pick Type C Disease
Authors: Maeda Yuki , Motoyama Keiichi , Nishiyama Rena , Higashi Taishi , Onodera Risako , Nakamura Hideaki , Takeo Toru , Nakagata Naomi , Yamada Yusei , Ishitsuka Yoichi , Kondo Yuki , Irie Tetsumi , Era Takumi , Arima Hidetoshi
Published: May 2019

Niemann-Pick type C disease (NPC) is a fatal, autosomal recessive disorder, which causes excessive accumulation of free cholesterol in endolysosomes, resulting in progressive hepatomegaly and neurodegeneration. Currently, 2-hydroxypropyl-β-cyclodextrin (HP-β-CyD) is used at a high dose for the treatment of NPC, risking lung toxicity and hearing loss during treatment. One method to reduce the required dose of HP-β-CyD for the treatment of hepatomegaly is to actively deliver β-cyclodextrin (β-CyD) to hepatocytes. Previously, we synthesized lactosyl-β-CyD (Lac-β-CyD) and demonstrated that it lowers cholesterol in NPC model liver cells. In the present study, we studied the efficacy and safety of Lac-β-CyD treatment of hepatomegaly in Npc1−/− mice. After subcutaneous administration, Lac-β-CyD accumulated in the liver and reduced hepatomegaly with greater efficacy than HP-β-CyD. In addition, subcutaneous administration of a very high dose of Lac-β-CyD was less toxic to the lungs than HP-β-CyD. Notably, the accumulation of intracellular free cholesterol in endolysosomes of NPC-like liver cells was significantly lower after administration of Lac-β-CyD than after treatment with HP-β-CyD. In conclusion, these results suggest that Lac-β-CyD is a candidate for the effective treatment of hepatomegaly in NPC.
Controlled Growth of LDH Films with Enhanced Photocatalytic Activity in a Mixed Wastewater Treatment
Authors: Wang Zhongchuan , Fang Pengfei , Kumar Parveen , Wang Weiwei , Liu Bo , Li Jiao
Published: May 2019

Due to multiple charge transport pathways, adjustable layer spacing, compositional flexibility, low manufacturing cost, and absorption of visible light, layered double hydroxides (LDHs) are a promising material for wastewater treatment. In this study, LDH films and Fe-doped LDH films with different metal ions (Ni, Al, Fe) on the surface of conductive cloth were successfully prepared and applied for the photocatalytic degradation of wastewater containing methyl orange and Ag ions under visible-light irradiation. The chemical state of Fe ions and the composition of LDHs on methyl orange photodegradation were investigated. The experimental results showed that LDH films exhibited high photocatalytic activity. The photocatalytic activity of LDH films on methyl orange improved in the mixed wastewater, and the Fe-doped NiAl–LDH films exhibited best visible-light photocatalytic performance. The analysis showed that Ag ions in the mixed wastewater were reduced by the LDH films and subsequently deposited on the surface of the LDH films. The Ag nanoparticles acted as electron traps and promoted the photocatalytic activity of the LDH films on methyl orange. Thus, we have demonstrated that prepared LDH films can be used in the treatment of mixed wastewater and have broad application prospects in environmental remediation and purification processes.
May Young Elite Cyclists Have Less Efficient Bone Metabolism?
Authors: Rapún-López Marta , Olmedillas Hugo , Gonzalez-Agüero Alejandro , Gomez-Cabello Alba , Pradas de la Fuente Francisco , Moreno Luis A. , Casajús José A. , Vicente-Rodríguez Germán
Published: May 2019

The purpose of this work was to describe changes in metabolic activity in the bones of young male competitive cyclists (CYC) as compared with age-matched controls (CON) over a one-year period of study. Eight adolescent male cyclists aged between fourteen and twenty, and eight age-matched controls participated in this longitudinal study. Serum osteocalcin (OC), amino-terminal propeptide of type I procollagen (PINP), beta-isomerized C-telopeptides (β-CTx) and plasma 25 hydroxyvitamin D [25(OH)D], were investigated by an electrogenerated chemiluminescence immunoassay. Analysis of variance revealed no significant differences in formation and resorption markers between cyclists and controls. Within the groups, both CYC and CON showed decreased OC at −30% and −24%, respectively, and PINP where the figures were −28% and −30% respectively (all p < 0.05). However, only the CYC group showed a decrease in [25(OH)D], lower by 11% (p < 0.05). The similarity in the concentrations of markers in cyclists and controls seems to indicate that cycling does not modify the process of bone remodeling. The decrease in vitamin D in cyclists might be detrimental to their future bone health.
Extract Methods, Molecular Characteristics, and Bioactivities of Polysaccharide from Alfalfa (Medicago sativa L.)
Authors: Zhang Chen , Li Zemin , Zhang Chong-Yu , Li Mengmeng , Lee Yunkyoung , Zhang Gui-Guo
Published: May 2019

The polysaccharide isolated from alfalfa was considered to be a kind of macromolecule with some biological activities; however, its molecular structure and effects on immune cells are still unclear. The objectives of this study were to explore the extraction and purifying methods of alfalfa (Medicago sativa L.) polysaccharide (APS) and decipher its composition and molecular characteristics, as well as its activation to lymphocytes. The crude polysaccharides isolated from alfalfa by water extraction and alcohol precipitation methods were purified by semipermeable membrane dialysis. Five batches of alfalfa samples were obtained from five farms (one composite sample per farm) and three replicates were conducted for each sample in determination. The results from ion chromatography (IC) analysis showed that the APS was composed of fucose, arabinose, galactose, glucose, xylose, mannose, galactose, galacturonic acid (GalA), and glucuronic acid (GlcA) with a molar ratio of 2.6:8.0:4.7:21.3:3.2:1.0:74.2:14.9. The weight-average molecular weight (Mw), number-average molecular weight (Mn), and Z-average molecular weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, respectively, according to the analysis by gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS). The findings of electron ionization mass spectrometry (EI-MS) suggest that APS consists of seven linkage residues, namely 1,5-Araf, galactose (T-D-Glc), glucose (T-D-Gal), 1,4-Gal-Ac, 1,4-Glc, 1,6-Gal, and 1,3,4-GalA, with molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, respectively. Additionally, APS markedly increased B-cell proliferation and IgM secretion in a dose- and time-dependent manner but not the proliferation and cytokine (IL-2, -4, and IFN-γ) expression of T cells. Taken together, the present results suggest that APS are macromolecular polymers with a molar mass (indicated by Mw) of 3.3 × 106 g/mol and may be a potential candidate as an immunopotentiating pharmaceutical agent or functional food.
High Chili Intake and Cognitive Function among 4582 Adults: An Open Cohort Study over 15 Years
Authors: Shi Zumin , El-Obeid Tahra , Riley Malcolm , Li Ming , Page Amanda , Liu Jianghong
Published: May 2019

We aimed to examine the association between chili intake and cognitive function in Chinese adults. This is a longitudinal study of 4852 adults (age 63.4 ± 7.7) attending the China Health and Nutrition Survey during 1991 and 2006. Cognitive function was assessed in 1997, 2000, 2004 and 2006. In total, 3302 completed cognitive screening tests in at least two surveys. Chili intake was assessed by a 3-day food record during home visits in each survey between 1991 and 2006. Multivariable mixed linear regression and logistic regression were used. Chili intake was inversely related to cognitive function. In fully adjusted models, including sociodemographic and lifestyle factors, compared with non-consumers, those whose cumulative average chili intake above 50 g/day had the regression coefficients (and 95% CI) for global cognitive function of −1.13 (−1.71–0.54). Compared with non-consumers, those with chili consumption above 50 g/day had the odds ratio (and 95% CI) of 2.12(1.63–2.77), 1.56(1.23–1.97) for self-reported poor memory and self-reported memory decline, respectively. The positive association between chili intake and cognitive decline was stronger among those with low BMI than those with high BMI. The longitudinal data indicate that higher chili intake is positively associated with cognitive decline in Chinese adults in both genders.
Cucurbita argyrosperma Seed Extracts Attenuate Angiogenesis in a Corneal Chemical Burn Model
Authors: Estrella-Mendoza María Fernanda , Jiménez-Gómez Francisco , López-Ornelas Adolfo , Pérez-Gutiérrez Rosa Martha , Flores-Estrada Javier
Published: May 2019

Severe corneal inflammation produces opacity or even perforation, scarring, and angiogenesis, resulting in blindness. In this study, we used the cornea to examine the effect of new anti-angiogenic chemopreventive agents. We researched the anti-angiogenic effect of two extracts, methanol (Met) and hexane (Hex), from the seed of Cucurbita argyrosperma, on inflamed corneas. The corneas of Wistar rats were alkali-injured and treated intragastrically for seven successive days. We evaluated: opacity score, corneal neovascularization (CNV) area, re-epithelialization percentage, and histological changes. Also, we assessed the inflammatory (cyclooxigenase-2, nuclear factor-kappaB, and interleukin-1β) and angiogenic (vascular endothelial growth factor A, VEGF-A; -receptor 1, VEGFR1; and -receptor 2, VEGFR2) markers. Levels of Cox-2, Il-1β, and Vegf-a mRNA were also determined. After treatment, we observed a reduction in corneal edema, with lower opacity scores and cell infiltration compared to untreated rats. Treatment also accelerated wound healing and decreased the CNV area. The staining of inflammatory and angiogenic factors was significantly decreased and related to a down-expression of Cox-2, Il-1β, and Vegf. These results suggest that intake of C. argyrosperma seed has the potential to attenuate the angiogenesis secondary to inflammation in corneal chemical damage.
Endothelial Function is improved by Inducing Microbial Polyamine Production in the Gut: A Randomized Placebo-Controlled Trial
Authors: Matsumoto Mitsuharu , Kitada Yusuke , Naito Yuji
Published: May 2019

Recently, it was demonstrated that spermidine-induced autophagy reduces the risk of cardiovascular disease in mice. Intestinal bacteria are a major source of polyamines, including spermidine. We previously reported that the intake of both Bifidobacterium animalis subsp. lactis (Bifal) and arginine (Arg) increases the production of putrescine, a spermidine precursor, in the gut. Here, we investigated the effects of Bifal and Arg consumption on endothelial function in healthy subjects. Healthy individuals with body mass index (BMI) near the maximum value in the “healthy” range (BMI: 25) (n = 44) were provided normal yogurt containing Bifal and Arg (Bifal + Arg YG) or placebo (normal yogurt) for 12 weeks in this randomized, double-blinded, placebo-controlled, parallel-group comparative study. The reactive hyperemia index (RHI), the primary outcome, was measured using endo-peripheral arterial tone (EndoPAT). The change in RHI from week 0 to 12 in the Bifal + Arg YG group was significantly higher than that in the placebo group, indicating that Bifal + Arg YG intake improved endothelial function. At week 12, the concentrations of fecal putrescine and serum putrescine and spermidine in the Bifal + Arg YG group were significantly higher than those in the placebo group. This study suggests that consuming Bifal + Arg YG prevents or reduces the risk of atherosclerosis.
Health-Promoting Components in Fermented Foods: An Up-to-Date Systematic Review
Authors: Melini Francesca , Melini Valentina , Luziatelli Francesca , Ficca Anna Grazia , Ruzzi Maurizio
Published: May 2019

Fermented foods have long been produced according to knowledge passed down from generation to generation and with no understanding of the potential role of the microorganism(s) involved in the process. However, the scientific and technological revolution in Western countries made fermentation turn from a household to a controlled process suitable for industrial scale production systems intended for the mass marketplace. The aim of this paper is to provide an up-to-date review of the latest studies which investigated the health-promoting components forming upon fermentation of the main food matrices, in order to contribute to understanding their important role in healthy diets and relevance in national dietary recommendations worldwide. Formation of antioxidant, bioactive, anti-hypertensive, anti-diabetic, and FODMAP-reducing components in fermented foods are mainly presented and discussed. Fermentation was found to increase antioxidant activity of milks, cereals, fruit and vegetables, meat and fish. Anti-hypertensive peptides are detected in fermented milk and cereals. Changes in vitamin content are mainly observed in fermented milk and fruits. Fermented milk and fruit juice were found to have probiotic activity. Other effects such as anti-diabetic properties, FODMAP reduction, and changes in fatty acid profile are peculiar of specific food categories.
Energy Metabolism and Intermittent Fasting: The Ramadan Perspective
Authors: Lessan Nader , Ali Tomader
Published: May 2019

Intermittent fasting (IF) has been gaining popularity as a means of losing weight. The Ramadan fast (RF) is a form of IF practiced by millions of adult Muslims globally for a whole lunar month every year. It entails a major shift from normal eating patterns to exclusive nocturnal eating. RF is a state of intermittent liver glycogen depletion and repletion. The earlier (morning) part of the fasting day is marked by dominance of carbohydrate as the main fuel, but lipid becomes more important towards the afternoon and as the time for breaking the fast at sunset (iftar) gets closer. The practice of observing Ramadan fasting is accompanied by changes in sleeping and activity patterns, as well as circadian rhythms of hormones including cortisol, insulin, leptin, ghrelin, growth hormone, prolactin, sex hormones, and adiponectin. Few studies have investigated energy expenditure in the context of RF including resting metabolic rate (RMR) and total energy expenditure (TEE) and found no significant changes with RF. Changes in activity and sleeping patterns however do occur and are different from non-Ramadan days. Weight changes in the context of Ramadan fast are variable and typically modest with wise inter-individual variation. As well as its direct relevance to many religious observers, understanding intermittent fasting may have implications on weight loss strategies with even broader potential implications. This review examines current knowledge on different aspects of energy balance in RF, as a common model to learn from and also map out strategies for healthier outcomes in such settings.
High Relative Abundance of Lactobacillus reuteri and Fructose Intake are Associated with Adiposity and Cardiometabolic Risk Factors in Children from Mexico City
Authors: Huerta-Ávila Eira E. , Ramírez-Silva Ivonne , Torres-Sánchez Luisa E. , Díaz-Benítez Cinthya E. , Orbe-Orihuela Yaneth C. , Lagunas-Martínez Alfredo , Galván-Portillo Marcia , Flores Mario , Cruz Miguel , Burguete-García Ana I.
Published: May 2019

In Mexico, 3 of 10 children are overweight. Fructose intake and relative abundance (RA) of Lactobacillus reuteri (L. reuteri) in the intestinal microbiota are associated with obesity and diabetes in adults, but studies in children are limited. This study evaluates the association between fructose intake and L. reuteri RA with adiposity and cardiometabolic risk markers in Mexican children dietary information, microbiota profiles, adiposity indicators (Body Mass Index, BMI and Waste Circumference, WC), and cardiometabolic markers were analyzed in 1087 children aged 6–12 years. Linear regression and path analysis models were used. High-tertile fructose intake and L. reuteri RA were positively associated with BMI (βTertil 3 vs. Tertil 1 = 0.24 (95% CI, 0.04; 0.44) and βT3 vs. T1 = 0.52 (95% CI, 0.32; 0.72)) and WC (βT3 vs. T1 = 2.40 (95% CI, 0.93; 3.83) and βT3 vs. T1 = 3.40 (95% CI, 1.95; 4.90)), respectively. Also, these factors mediated by adiposity were positively correlated with high triglycerides and insulin concentrations and HOMA-IR (p ≤ 0.03) and negatively associated with HDL-C concentration (p < 0.01). High-tertile fructose intake and L. reuteri RA were directly associated with adiposity and indirectly associated though adiposity with metabolic disorders in children. In conclusion, fructose intake and L. reuteri RA were directly associated with adiposity and indirectly associated with metabolic disorders in children, mediated by adiposity.
Cardiovascular Risk Factors and Their Association with Vitamin D Deficiency in Mexican Women of Reproductive Age
Authors: Contreras-Manzano Alejandra , Villalpando Salvador , García-Díaz Claudia , Flores-Aldana Mario
Published: May 2019

Based on a nationally representative sample of young Mexican women aged 20 to 49 years (n = 3260), we sought to explore whether cardiovascular risk factors and acute myocardial infarction (AMI) were associated with vitamin D deficiency (VDD, defined as 25-OH-D <50 nmol/L). To this end, we obtained sociodemographic, serum and anthropometric data from the 2012 National Health and Nutrition Survey (ENSANUT 2012). Analyses were developed through logistic regression models adjusted for potential confounders. The prevalence of VDD was significantly higher in obese women (42.5%, 95% CI; 37.3–47.9) compared to women with a normal body mass index (29.9%, 95% CI; 23.5–37.1, p = 0.05), in those with high total cholesterol (TC) (45.6% 95% CI; 39.4–51.9) compared to those with normal TC levels (33.9%, 95% CI 30–38.1, p = 0.03), and in those with insulin resistance (IR) (44%, 95% CI; 36.9–51.7) or type 2 diabetes mellitus (T2DM) (58.6%, 95% CI 46.9–69.4) compared to those with normal glycemia (no insulin resistance: 34.7%, 95% CI; 30.9–38.8, p = 0.04 and no T2DM: 34.9%, 95% CI 31.4–38.6, p < 0.001). Utilizing individual models to estimate cardiovascular risk according to VDD, we found that the odds of being obese (odds ratio, OR: 1.53, 95% CI 1.02–2.32, p = 0.05), or having high TC levels (OR: 1.43, 95% CI; 1.05–2.01, p = 0.03), T2DM (OR: 2.64, 95% CI; 1.65–4.03, p < 0.001), or IR (OR: 1.48, 95% CI 1.04–2.10, p = 0.026) were significantly higher in women with VDD (p < 0.05). Odds were not statistically significant for overweight, high blood pressure, sedentarism, AMI, high serum concentration of triglycerides, homocysteine, or C-reactive protein models. In conclusion, our results indicate that young Mexican women with VDD show a higher prevalence of cardiovascular risk factors.
pH-Responsive i-motif Conjugated Hyaluronic Acid/Polyethylenimine Complexes for Drug Delivery Systems
Authors: Lee Gyeong Jin , Kim Tae-il
Published: May 2019

i-motif is cytosine (C)-rich oligonucleotide (ODN) which shows pH-responsive structure change in acidic condition. Therefore, it has been utilized for the trigger of intercalated drug release, responding to environmental pH change. In this study, 2.76 molecules of i-motif binding ODNs (IBOs) were conjugated to each hyaluronic acid (HA) via amide bond linkages. Synthesis of HA-IBO conjugate (HB) was confirmed by FT-IR and agarose gel electrophoresis with Stains-All staining. After hybridization of HB with i-motif ODN (IMO), it was confirmed that doxorubicin (DOX) could be loaded in HB-IMO hybrid structure (HBIM) with 65.6% of drug loading efficiency (DLE) and 25.0% of drug loading content (DLC). At pH 5.5, prompt and significant DOX release from HBIM was observed due to the disruption of HBIM hybrid structure via i-motif formation of IMO, contrary to pH 7.4 condition. Then, HBIM was complexed with low molecular weight polyethylenimine (PEI1.8k), forming positively charged nanostructures (Z-average size: 126.0 ± 0.4 nm, zeta-potential: 16.1 ± 0.3 mV). DOX-loaded HBIM/PEI complexes displayed higher anticancer efficacy than free DOX in A549 cells, showing the potential for pH-responsive anticancer drug delivery systems.
Fulfilling Educational Competencies through Global Pharmacy Experiences
Authors: Childs-Kean Lindsey M. , Motycka Carol , Normann Sven A. , Doty Randell
Published: May 2019

Many Colleges of Pharmacy in the United States offer international education and practice experiences to their students. Multiple publications have described these offerings and related them back to the CAPE 2013 Outcomes. This article describes the multiple international programs offered by one College of Pharmacy, including international Advanced Pharmacy Practice Experiences, Short Study Abroad Programs, and International Health Outreach Trips. The article also details the relevant competencies associated with these international experiences.
Promote Localized Surface Plasmonic Sensor Performance via Spin-Coating Graphene Flakes over Au Nano-Disk Array
Authors: Alharbi Raed , Yavuz Mustafa
Published: May 2019

Although localized surface plasmonic resonance (LSPR) sensors have advantages over regular surface plasmonic resonance (SPR) sensors, such as in sensor setup, excitation method, and cost, they suffer from low performance when compared to SPR sensors, which thus limits their commercialization. Among different methods applied to promote LSPR sensor performance, metal-two-dimensional (2D) hybrid nanostructure has been shown to be an efficient improvement. However, metal-2D hybrid nanostructures may come in a complex or a simple scheme and the latter is preferred to avoid challenges in fabrication work and to be applicable in mass production. In this work, a new and simple gold-graphene hybrid scheme is proposed and its plasmonic sensing performance is numerically evaluated using the finite different time domain (FDTD) method. The proposed sensor can be fabricated by growing a Au nano-disk (ND) array on a quartz substrate and then spin-coating graphene flakes of different sizes and shapes randomly on top of and between the Au NDs. Very high sensitivity value is achieved with 2262 nm/RIU at a 0.01 refractive index change. The obtained sensitivity value is very competitive in the field of LSPR sensors using metal-2D hybrid nanostructure. This proposed sensor can be utilized in different biosensing applications such as immunosensors, sensing DNA hybridization, and early disease detection, as discussed at the end of this article.
Low-Energy State Electron Beam in a Uniform Channel
Authors: Fuks Mikhail , Andreev Dmitrii , Kuskov Artem , Schamiloglu Edl
Published: May 2019

In our earlier work, we showed that a low-energy state of an electron beam exists in a nonuniform channel between two virtual cathodes in a magnetron with diffraction output, which consists of three uniform sections with increasing radius. A uniform axial magnetic field fills the interaction space. This led to magnetron operation with >90% efficiency when combined with a magnetic mirror field at the output end. In this present paper, we show that a low-energy state of an electron beam can be realized in a uniform channel in which an increasing magnetic field is used in order to create a magnetic mirror at the output end. We consider two cases, one where the injected beam current slightly exceeds the space-charge-limiting current and the other where the injected beam current greatly exceeds the space-charge-limiting current. On the time scale of relevance to planned experiments (∼30 ns), when the injected current slightly exceeds the space-charge-limiting current a stationary virtual cathode forms and when the injected current greatly exceeds the space-charge-limiting current the virtual cathode oscillates back and forth.