bluuebunny/crossref_metadata_2025 huggingface dataset:
Probabilistic Sea Level Rise Flood Projections Using a Localized Ocean Reference Surface
Authors: Paoa-Kannegiesser Noah A. , Fletcher Charles H. , Anderson Tiffany R. , Coffman Makena
Published: January 2022

Projecting sea level rise (SLR) impacts requires defining ocean surface variability as a source of uncertainty. We analyze data from a Regional Ocean Modeling System (ROMS) reanalysis for the region surrounding the main Hawaiian Islands to incorporate the ocean surface uncertainty in mapping SLR flood probabilities. By analyzing the ocean surface height component of the ROMS reanalysis, we create an ocean surface reference (ORS) as a proxy for MHHW. We model the NOAA Intermediate, Intermediate-high and High regional SLR scenarios for the years 2050 and 2100 at three field sites around Oʻahu; Waikīkī, Hauʻula, Haleʻiwa. We calculate a probability density function (PDF) by convolving the PDF of water level derived from the ROMS reanalysis data with the PDF of error associated with a digital elevation model of the study sites. The resulting joint-PDF of flood depth allows us to create two types of probability-based flood projections: (1) Maps illustrating varying flood depths for a given probability threshold and, (2) maps illustrating varying probability for a specific flood depth. We compare 80% probability flood projections using our ORS approach to projections using the TCARI grid, the standard NOAA method. We highlight the importance of uncertainty and user-defined probability in identifying pixels that function as tipping points distinguishing flooding styles.
Mechanistic Study of Silica Nanoparticles on the Size-dependent Retinal Toxicity in Vitro and in Vivo
Authors: Zhang Zhuhong , Zhao Laien , Ma Yuanyuan , Liu Jia , Huang Yanmei , Fu Xiaoxuan , Peng Shengjun , Wang Xiaojie , Yang Yun , Zhang Xiaoyan , Ding Wanru , Yu Jinguo , Zhu Yanping , Yan Hua , Yang Shubin
Published: January 2022

Background: Silica nanoparticles (SiO2 NPs) are extensively applied in the biomedical ﬁeld. The increasing medical application of SiO2 NPs has raised concerns about their safety. However, studies on SiO2 NP-induced retinal toxicity are lacking.Methods: We investigated the retinal toxicity of SiO2 NPs with different sizes (15 and 50 nm) in vitro and in vivo along with the underlying mechanisms. The cytotoxicity of SiO2 NPs with different sizes was assessed in R28 human retinal precursor cells by determining the ATP content and LDH release. The cell morphologies and nanoparticle distributions in the cells were analyzed by phase-contrast microscopy and transmission electron microscopy, respectively. The mitochondrial membrane potential was examined by confocal laser scanning microscopy. The retinal toxicity induced by SiO2 NPs in vivo was examined by immunohistochemical analysis. To further investigate the mechanism of retinal toxicity induced by SiO2 NPs, reactive oxygen species (ROS) generation, glial cell activation and inflammation were monitored.Results: The 15-nm SiO2 NPs were found to have higher cytotoxicity than the larger NPs. Notably, the 15-nm SiO2 NPs induced retinal toxicity in vivo, as demonstrated by increased cell death in the retina, TUNEL-stained retinal cells, retinal ganglion cell degeneration, glial cell activation, and inflammation. In addition, The SiO2 NPs caused oxidative stress, as demonstrated by the increase in the ROS indicator H2DCF-DA. Furthermore, the pretreatment of R28 cells with N-acetylcysteine, an ROS scavenger, attenuated the ROS production and cytotoxicity induced by SiO2 NPs.Conclusions: These results provide evidence that SiO2 NPs induce size-dependent retinal toxicity and suggest that glial cell activation and ROS generation contribute to this toxicity.
Apolipoprotein C3 and Necrotic Core Volume Are Correlated but Also Associated With Future Cardiovascular Events
Authors: Ohwada Takayuki , Sakamoto Takayuki , Suzuki Satoshi , Sugawara Yukiko , Nakazato Kazuhiko , Takeishi Yasuchika , Watanabe Kenichi , Sakamoto Kazuya , Ikeda Ayano , Haga Fumika
Published: January 2022

We aimed to clarify the relationship between apolipoprotein C3 (apo-C3) and vascular composition of lesion plaque in stable coronary disease (SCD) before percutaneous coronary intervention (PCI) and to investigate major adverse cardiovascular events (MACEs) within 4 years. Data of 98 consecutive patients with SCD who underwent PCI between November 1, 2012, and March 10, 2015, were analyzed. Laboratory evaluation and virtual histology-intravascular ultrasound (VH-IVUS) examination of culprit lesions were conducted before PCI. Patients were divided according to the median apo-C3 value into low apo-C3 (≤8.5 mg/dL) and high apo-C3 (>8.5 mg/dL) groups. VH-IVUS data indicated that the percentage of necrotic core volume (%NC) was significantly higher in the high apo-C3 group than in the low apo-C3 group. Moreover, the %NC significantly correlated with the apo-C3 level (R=0.2109, P=0.037). Kaplan–Meier curve analysis revealed that freedom from MACEs decreased more in the high apo-C3 group than in the low apo-C3 group and in the high %NC group than in the low %NC group. Multivariate Cox hazards analysis showed that the %NC and high apo-C3 were independent predictors of 4-year MACEs. Apo-C3 may be a useful marker for future MACEs in patients with SCD after PCI and contribute to %NC growth.
DNA-Methylation-Mediated lncRNA HOXB-AS4 Promotes Gastric Cancer Progression Through Regulating miR-130a-5p/PKP4
Authors: Wang Xiaoyan , He Rong , Wang Yan , Liu Yunyun , Wang Yuxin , Chen Dandan , Fan Yu
Published: January 2022

Background：Gastric cancer (GC) is one of the most common cancer in the world, possessing the second leading cause of cancer-related mortality. Long noncoding RNAs (lncRNAs) have been shown to play important roles in tumorigenesis. However, the effect of lncRNA HOXB-AS4 in GC progression and the underlying mechanisms remain unknown. Methods：Firstly, the expression of lncRNA HOXB-AS4 in gastric cancer tissues and cancer cells was investigated according to GEPIA database and Real time fluorescence quantitative PCR(qRT-PCR). Then, MTT, clone formation, Transwell and Western blot were used to study the effects of overexpression or down-regulation of HOXB-AS4 on the proliferation, invasion and epithelial mesenchymal transformation of cancer cells. We further studied the molecular mechanism of HOXB-AS4 by fluorescence in situ hybridization, bioinformatics analysis, luciferase reporting, methylation specific PCR (MSP) and chromatin immunoprecipitation (chip).Results:In the study, the GEPIA database and quantitative Real-Time PCR (qRT-PCR) assay showed that HOXB-AS4 was upregulated in GC tissues and cells. Then, MTT, clone formation, transwell, and western blot assays suggested that overexpression of HOXB-AS4 increased cell proliferation, migration, and invasion, and regulated epithelial-mesenchymal transition (EMT) markers expression, while knockdown of HOXB-AS4 showed the opposite effect. Fluorescence in situ hybridization (FISH) assay found that HOXB-AS4 localized in the cytoplasm of the GSE-1 and AGS cells. Further mechanism experiments, including bioinformatics, luciferase reporter, qRT-PCR, and western blot assays showed that HOXB-AS4 sponged to miR-130a-5p to regulate the PKP4 expression. Knockdown of miR-130a-5p obliterated the effect of HOXB-AS4, which was further abolished by knockdown of PKP4 in vitro and in vivo. Methylation-specific PCR (MSP) and chromatin immunoprecipitation (CHIP) assay showed that overexpression of HOXB-AS4 in GC was mediated by SP1-dependent DNA methylation. Abnormal upregulation of lncRNA HOXB-AS4 contributed to GC progression, which was mediated by DNA methylation. The study clarified that DNA-methylation-mediated HOXB-AS4 played its role through miR-130a-5p/PKP4 axis.Conclusions: Our study provides new insights for the understanding of epigenetic regulation on lncRNA expression in GC, and indicates that HOXB-AS4 could be a biomarker of GC prognosis. Moreover, targeting HOXB-AS4 /miR-130a-5p/PKP4 axis might be a promising strategy to treat GC.
Factors Associated With Pharmacological and Non-pharmacological Treatments Adherence in Patients With Borderline Personality Disorder in Iran
Authors: Mohammadabadi Motahareh Sadat Mirhaj , Mohammadsadeghi Homa , Adrebili Mehrdad Eftekhar , Kolour Zahra Partovi , Kashaninasab Fatemeh , Shalbafan Mohammadreza
Published: January 2022

BackgroundBorderline personality disorder is a major mental illness characterized by a sustained relationship instability, impulsive behavior and intense affects. Adherence is a complex behavior, from minor refusals of treatment to inappropriate use of health services or even abandonment of treatment, which can be affected by various factors. Therefore, the present study aimed to investigate the factors affecting pharmacological and non-pharmacological adherence in patients with borderline personality disorder referred to an outpatient referral clinic in Tehran, Iran.MethodsThe study was a retrospective cohort. The files of patients with borderline personality disorder referred to the outpatient clinic of the Tehran Psychiatric Institute were reviewed as the first step. In the next step, we contacted the patients and asked them to fill out the questionnaires. Data were collected using the Drug Attitude Inventory (DAI) questionnaire and a researcher made questionnaire to determine the attitude of patients toward pharmacological and non-pharmacological treatment and therapeutic adherence. After collecting data, patients’ therapeutic acceptance was divided into three groups: poor, partial, good compliance. The data were analyzed by SPSS software version-22.Results Ninety-four patients were involved in the study and fifty four of them were women. In terms of psychotherapy adherence, patients with higher education and hospital admission history have better compliance. Medication attitudes were negative in 54 patients (57.4%), while 40.4% of them stated that psychotherapy or counselling did not help their condition and showed a negative attitude toward non-pharmacological treatment. Additionally, psychotherapy good adherence of the patients (44.7%) was higher than medication good adherence (31.9%). The most common reasons for discontinuation of treatment were medication side effects (53.1%), dissatisfaction with the therapist (40.3%) and then fear of medication dependence (40%). The results showed no relationship between other demographic factors and treatment adherence.ConclusionsResults of the current study show that attitude toward psychotherapy is more positive than pharmacotherapy. In addition, according to the results, working on changeable factors such as patients’ fear of dependence to medication, dissatisfaction with the therapist, and medication side effects may improve patients' treatment adherence.
Structural Characterization, DFT Geometry Optimization, Cyclic Voltammetry and Biological Assay of (Tellurite-pyridine) Mixed Ligandcomplexesof Cd(II)
Authors: El-Hady M. N. Abd , Gomaa E. A. , Zaky Rania , Elafifi S. E.
Published: January 2022

This research work presents spectral characterizations (IR, 1H NMR and 13C NMR) of anionic tellurito Cd(II) complexes that prepared using cyanopyridine derivatives as a polydentate ligands. Also, X-ray based techniques involving (EDX and XRD) are applied for cadmium complexes to realize elemental composition and average crystallographic coherence. Moreover, the electrochemical studies represented on cyclic voltammetry are determined for Cd)II) in (absence/presence) of ligands to detect the role of complexation in solution measurements. All the previous experimental investigations are supported with molecular modeling of the geometric optimized structures based on density function theory (DFT) for all compounds accompanied by the calculations of different energetic parameters such as EHOMO and ELUMO. Finally, anti-microbial (antibacterial and antifungal), anti-oxidant and Bleomycin dependent DNA damage are screened for all samples to predict the influence of metal complex formation on the biological activity of pyridyl ligands besides their priority.
Expression of FAP in Lung Cancer and Its Correlation With the Tumor Glucose Metabolism and the Histopathology
Authors: Chen Xiaohui , Liu Xinran , Wang Lijuan , Zhou Wenlan , Zhang Yin , Tian Ying , Tan Jianer , Dong Ye , Fu Lilan , Wu Hu bing
Published: January 2022

Purpose To explore the expression of fibroblast activation protein (FAP) in lung cancer (LC) and its correlation with tumor glucose metabolism and histopathology.Methods From June 2018 to November 2020, a total of 73 patients with newly diagnosed LC were included. Immunohistochemical staining was used to quantify the FAP expression in the tumors. The histopathological type and tumor grade were determined on the histopathological examination. Tumor glucose metabolic parameters and tumor maximal diameter were measured on [18F] F-FDG PET/CT. Univariate and multivariate analysis were used to study the correlation between FAP expression level and glucose metabolic variables and various histopathology.Results Positive FAP expression was observed in 97.3% (71/73) of LC lesions, which was significantly higher than 87.7% (64/73) of [18F] F-FDG positivity on PET/CT (χ2=4.818, P=0.028). In 12 early adenocarcinomas (ADCs), only 3 lesions (25%) were positive on PET/CT, however, 10 lesions (83.3%) were positive with FAP expression. When FAP expression was classified to low level (scores≤3) and high level (scores>4), high FAP level was found in 80.8% tumors and low FAP level in other 19.2% tumors. High FAP level was identified in 100.0% of squamous cell carcinoma (SCC), 85.7% of ADCs, 66.7% (4/6) of large cell neuroendocrine carcinoma (LCNC) and 40.0% (4/10) of small cell lung cancer (SCLC)(P<0.05). In the non-mucinous ADC lesions, on univariate analysis, FAP expression level showed close relationships with tumor metabolism parameters (SUVmax, SUVmean, and TLG), tumor diameter, tumor grade and lesion attenuation (P<0.05).Conclusion The present study demonstrates that FAP expresses widely in LC and has a great variant level in different histopathological types. High positive rate of FAP expression implies FAP targeted imaging may be a sensitive modality for diagnosing LC, especially for early ADCs, and may serve as an alternative of [18F] F-FDG PET/CT.
Flavopiridol Protects Against Fungal Keratitis by Alleviating Inflammation Through the Promotion of Autophagy
Authors: Gu Lingwen , Li Cui , Peng Xudong , Lin Hao , Niu Yawen , Zheng Hengrui , Zhao Guiqiu , Lin Jing
Published: January 2022

Background: Fungal keratitis is a serious infectious keratopathy related to fungal virulence and excessive inflammatory responses. Autophagy exhibits a potent ability to resolve inflammation during fungal infection. This study aimed to investigate the protective function of flavopiridol in fungal keratitis and explore its effects on autophagy.Methods: A mouse model of fungal keratitis was established and then treated with 5 μM flavopiridol. RAW 264.7 cells were treated with 200 nM flavopiridol before fungal stimulation. The severity of corneal diseases was evaluated by slit-lamp microscopy. The expression levels of cytokines were detected by RT-PCR and ELISA. The protein levels of LC3, Beclin-1 and Atg7 were determined by western blot and immunofluorescence. A Cell Counting Kit-8 assay was used to test cell viability. Autolysosomes were detected by transmission electron microscopy (TEM). An inhibitor of autophagy, 3-methyladenine (3-MA), was used to pretreat RAW 264.7 cells. Phagocytosis of RAW 264.7 cells was evaluated by counting colony forming units. A. fumigatus was incubated with flavopiridol, and the hyphae were stained with calcofluor white. Absorbance assay, crystal violet staining and adherence assay were used to detect the antifungal activity of flavopiridol.Results: Flavopiridol treatment notably reduced corneal opacity and the clinical scores of infected corneas. Compared with DMSO treatment, flavopiridol treatment greatly downregulated IL-1β, IL-6 and TNF-a expression in infected corneas. In RAW 264.7 cells, flavopiridol treatment inhibited IL-1β, IL-6 and TNF-a expression but promoted IL-10 expression. TEM images showed that more autolysosomes were presented in infected corneas and RAW 264.7 cells after flavopiridol treatment than after DMSO treatment. Flavopiridol treatment notably upregulated the protein expression of LC3, Beclin-1 and Atg7 in infected corneas as well as in RAW 264.7 cells. 3-MA pretreatment counteracted the cytokine regulation induced by flavopiridol. Moreover, flavopiridol promoted the phagocytosis of RAW 264.7 cells. Flavopiridol also exhibited antifungal activity by restricting fungal growth and limiting fungal biofilm formation and conidial adhesion. Conclusions: Flavopiridol significantly alleviated the inflammation of fungal keratitis by activating autophagy. In addition, flavopiridol promoted the phagocytosis of RAW 264.7 cells and exhibited antifungal function, indicating the potential therapeutic role of flavopiridol in fungal keratitis.
Metformin and Calcitriol Enhance 5-Fluorouracil Tumoricidal Effects in Colon Cancer By Modulating The PI3K/Akt/PTEN/mTOR Network
Authors: Almaimani Riyad , Aslam Akhmed , Ahmad Jawwad , El-Readi Mahmoud Zaki , El-Boshy Mohamed , Abdelghany Abdelghany H. , Idris Shakir , Alhadrami Mai , Althubiti Mohammad , Almasmoum Hussain , Ghaith Mazen M , Abdallah Mohamed E , Eid Safaa Y , Refaat Bassem
Published: January 2022

Purpose: Chemoresistance to 5-Fluorouracil (5-FU) is common during colorectal cancer (CRC) treatment. This study measured the chemotherapeutic effects of 5-FU, calcitriol, and/or metformin single/dual/triple regimens as complementary/alternative therapies. Methods: Ninety male mice were divided into: negative and positive (PC) controls, 5-FU, Cal, Met, 5-FU/Cal, 5-FU/Met, Cal/Met, and 5-FU/Cal/Met groups. Treatments lasted four weeks following CRC induction by azoxymethane. The therapeutic regimens were also applied in the SW480 and SW620 CRC cell lines. Results: The PC mice had abundant tumours, markedly elevated proliferation markers (survivin/CCND1) and PI3K/Akt/mTOR alongside reduced p21/PTEN/Cytochrome-C/Caspase-3 and apoptosis. All therapies reduced tumour numbers, with 5-FU/Cal/Met most prominent regimen. All protocols also decreased cell proliferation markers, inhibited PI3K/Akt/mTOR molecules, increased pro-apoptotic molecules with apoptosis index, and 5-FU/Cal/Met revealed the strongest anti-cancer effects. In vitro, all therapies equally induced G1-phase arrest in SW480 cells, whereas metformin-alone showed maximal SW620 cell numbers in G0/G1-phase. 5-FU/Met co-therapy also showed the highest apoptotic SW480 cell numbers (13%), whilst 5-FU/Cal/Met disclosed the lowest percentage (81%) of viable SW620 cells. Moreover, 5-FU/Cal/Met revealed maximal inhibitions of cell cycle inducers (CCND1/CCND3), cell survival (BCL2) and the PI3K/Akt/mTOR molecules alongside highest expression of cell cycle inhibitors (p21/p27), pro-apoptotic markers (BAX/Cytochrome-C/Caspase-3), and PTEN in both cell lines. Conclusions: Metformin monotherapy was superior to calcitriol, whereas the 5-FU/metformin protocol showed better anti-cancer effects relative to the other dual therapies. However, the 5-FU/Cal/Met approach displayed the best in vivo and in vitro tumoricidal effects related to cell cycle arrest and apoptosis, justifiably by enhanced modulations of the PI3K/PTEN/Akt/mTOR pathway.
Plant Growth Modifications Due to Coastal Embankments Affect Soil Bacterial and Archaeal Communities Over the Growing Season in Salt Marshes of Eastern China
Authors: Feng Hongyu , Qiao Yajun , Xia Lu , Yang Wen , Zhao Yongqiang , Jeelani Nasreen , An Shuqing
Published: January 2022

Aims: Although the influences of coastal embankments on physicochemical soil properties and carbon (C) and nitrogen (N) cycling have been widely studied, the mechanisms of their effects on soil microbial ecologies remain poorly understood. Thus, the aim of this study was to investigate variations in the diversity and composition of soil bacterial and archaeal communities between natural and embanked saltmarshes, as well as the determinants that drive these variations.Methods: 16S rRNA gene sequence analysis was performed to assess the impacts of embankments on the bacterial and archaeal communities of native Suaeda salsa, Phragmites australis, and invasive Spartina alterniflora saltmarshes on the east coast of China.Results: Embankments were found to significantly decrease the microbial diversity of the S. alterniflora salt marsh, while they increased the OTU richness of the P. australis salt marsh. Embankments modified the compositions of soil bacterial and archaeal communities in both the S. alterniflora and P. australis salt marshes. However, variations in the microbial diversity, richness, and community compositions between the native and embanked S. salsa salt marshes were insignificant. Conclusions: These results were possibly because the embankment significantly altered soil nutrient substrate levels (e.g., soil organic C and N) by variations in plant residues and physiochemical soil properties in S. alterniflora and P. australis saltmarshes, whereas the embankment had no observable changes in the soil nutrient substrate and the plant residue in S. salsa saltmarsh. This study also elucidated the effects of coastal embankments on biogeochemical cycles, and highlighted their potential hazards to ecosystems.
Overexpression of Rice Gene Lsi1 (Low Silicon Gene 1) Enhances The Plant-Microbe Interaction Resulting In Improved Chilling Tolerance
Authors: Xie Yuebin , Khan Muhammad Umar , Lan Chaojie , Weng Peiying , Zou Jingnan , Wu Xin , Lin Wenxiong , Li Zhong
Published: January 2022

Chilling is an environmental phenomenon that hampers the plant growth. Related studies are mainly on based on the aerial plant parts. While, below ground to rhizosphere microbiome have been neglected under low temperature stress. The overexpression of Lsi1 in Dular significantly enhances its proline concentration compared with wild type Dular. This overexpression phenomenon maintains the osmotic balance of cells through influx of Ca2+, K+, H+ and efflux of Na+. The calcium deposition and the activity of plasma membrane H+-ATPase determined in root tip was consistent with the obtained ion flux results. In addition, the high-throughput sequencing results showed significant variation among identified 84 genera in different rhizocompartments (rhizosphere, rhizoplane and endosphere). The identified bacteria were associated with photosynthesis, energy metabolism, nitrogen fixation and defense, which were significantly increased in overexpressed plant. While, the number of pathogenic microorganisms associating were significantly reduced. Our findings suggests that the stability of ion balance in overexpressed plant roots affects the structure of microbial community in soil. To conclude, the over expression of Lsi1 gene enhanced cold tolerance of rice and its underlying mechanisms were comprehensively analyzed which can further impart its role in the stress tolerant rice plants.
Oxidative Stress Induction By Pesticides May Cause Lung Cancer Incidence
Authors: Asadikaram Gholamreza , Pourghdamyari Hossein , Abolhassani Moslem , Abbasi-Jorjandi Mojtaba , Faramarz Sanaz , Yousefi Fatemeh , Salimi Fouzieh , Afshar Reza Malekpour , Asadikaram Parisa , Shafiepour Mohsen
Published: January 2022

Background and aims:

Pesticides are nowadays known as one of the most important causes of human disorders worldwide. The aim of the present study was to investigate the role of organochlorine pesticides (OCPs) and organophosphorus pesticides (OPPs) in the development of lung cancer.
Methods

We determined the levels of seven derived OCP residues (α-HCH, β-HCH, γ-HCH, 2,4 DDT, 4,4 DDT, 2,4 DDE, and 4,4 DDE) and enzymatic antioxidant biomarkers including paraoxonase-1 (PON-1), erythrocyte's acetylcholinesterase (AChE), glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), and non-enzymatic antioxidant biomarkers including total antioxidant capacity (TAC), protein carbonyl (PC), malondialdehyde (MDA), and nitric oxide (NO) in the blood samples of 51 lung cancer patients and 51 healthy subjects as controls. Furthermore, the effects of OPP exposure on the development of lung cancer and oxidative stress (OS) are indirectly assessed by measuring AChE and PON-1 enzyme activities.
Results

The average values of all the measured OCPs were significantly higher in lung cancer patients when compared with healthy control subjects. AChE, PON-1, GPx, and CAT activity levels as well as the amounts of PC, MDA, and NO were higher in patients with lung cancer than in the control subjects, while TAC values were lower in the patients. Moreover, our data showed a significant association between OCP concentrations and OS parameters.
Conclusion

The results suggest that OCPs and OPPs may have a role in lung cancer incidence in southeastern Iran, and at least one of the mechanisms by which OCPs and OPPs may contribute to increasing the development of lung cancer in the studied area is through OS generation.
Clinical Factors Associated With Failed Weaning From Intraoperative Extracorporeal Membrane Oxygenation Used During Lung Transplantation
Authors: Choi Ji Yeon , Kim Song Yee , Leem Ah Young , Chung Kyung Soo , Lee Sang Hoon , Kim Eun Young , Jung Ji Ye , Kang Young Ae , Park Moo Suk , Kim Young Sam , Lee Jin Gu , Paik Hyo Chae , Lee Su Hwan
Published: January 2022

Background: Extracorporeal membrane oxygenation (ECMO) promotes adequate oxygenation and hemodynamic stability during lung transplantation (LTx). However, some recipients cannot be weaned from ECMO following surgery. Thus, we evaluated the prognosis and risk factors of failed weaning from intraoperative ECMO during LTx.Methods: We retrospectively analyzed data from 274 patients receiving intraoperative ECMO during LTx. Risk factors were evaluated using logistic regression analyses.Results: Weaning failure occurred in 118 patients (43.1%). Intensive care unit stay was longer and mortality was higher in the failed weaning group than in the successful weaning group. The failed weaning group exhibited significantly older donor age, lower donor PaO2/FiO2 ratio, greater intraoperative transfusion volume, and longer operation time than the successful weaning group. Recipient age, body mass index, donor age, lower donor PaO2/FiO2 ratio, donor/recipient total lung capacity (TLC) ratio, greater intraoperative transfusion volume, and longer operation time were associated with weaning failure after adjustment. Conclusion: The failed weaning group showed a poor prognosis. Perioperative factors including donor age, donor PaO2/FiO2 ratio, donor/recipient TLC, operation time, and blood loss can predict postoperative ECMO weaning failure.
A Special Extrusion-shear Manufacturing Method for Magnesium Alloy Rods Based on Finite Element Numerical Simulation and Experimental Verification
Authors: Zhang Ou , Hu Hongjun , Zhang Huiling , Zhao Hui , Zhang Ding-fei , Ou Zhongwen
Published: January 2022

To research the influences of process parameters on a special extrusion-shearmanufacture method for magnesium alloy rods, deform-3d software with finite elementsimulations has been used to analyze the material flows of deformed magnesium alloysAZ31B during the extrusion-shear (ES) process, as well as the grain sizes anddistribution of extrusion loads, stresses and strains, and blank temperatures. Temperaturefields, stress fields, strain fields and temperature fields varying with different blankpreheating temperatures, extrusion speed and extrusion ratios were simulated. Influences ofdifferent extrusion conditions and different die structures on microstructures of rods prepared by ES process has been researched. Extrusion forces decrease with the increasing extrusion temperatures, decreasing extrusion ratios, increasing die channel angles and decreasing friction coefficients. The flow velocities of metal in the ES die increase with development of ES process. Increasing the channel angles and reducing the friction factors would increase the outflow velocities of metal, but it has little effect on the uniformity of metal flow. The increase in friction and extrusion speed would increase the temperatures of the ES die. The ES process can prepare finer and more uniform microstructures than those prepared by direct extrusion under the same conditions.
The Vacuolar Transporter OsNRAMP2 Mediates Fe Remobilization During Germination and Affects Cd Distribution to Rice Grain
Authors: Chang Jia-Dong , Xie Yun , Zhang Huanhuan , Zhang Shurui , Zhao Fangjie
Published: January 2022

Background and aims Iron (Fe) deficiency in plants is a common problem affecting agricultural production. Cadmium (Cd) is a toxic metal that can be taken up and transported within plants by transporters for divalent metals including Fe(II). The present study aims to investigate the functions of OsNRAMP2 (Natural Resistance-Associated Macrophage Protein 2) in the remobilization and distribution of Fe and Cd in rice. Methods The expression pattern of OsNRAMP2 was determined by quantitative real-time PCR and pOsNRAMP2:GUS assay. Knockout mutants of OsNRAMP2 were generated by using CRISPR/Cas9 gene editing. Localization of Fe in the vacuolar globoids of germinating seeds was imaged by high-resolution transmission electron microscopy coupled with energy-dispersive X-ray spectroscopy. Distributions of Fe and Cd between different plant tissues were investigated in hydroponic and soil pot experiments. Results OsNRAMP2 was mainly expressed in the embryo of germinating seeds, roots, leaf sheaths and leaf blades. OsNRAMP2 was localized at the tonoplast. Knockout of OsNRAMP2 delayed seed germination and produced chlorotic seedling leaves. Remobilization of Fe stored in the protein storage vacuoles in the scutellum of germinating seeds was restricted in osnramp2 mutants compared with wild type. Expression of genes related to Fe uptake was enhanced in the seedlings of osnramp2 mutants. Knockout of OsNRAMP2 significantly decreased the distribution of Cd, but not Fe, from leaves and straws to rice grains. Conclusions OsNRAMP2 plays an important role in remobilizing vacuolar Fe during seed germination and affects translocation of Cd from vegetative tissues to rice grains.
WITHDRAWN: Relative Frequency of Islet Autoimmunity in Children and Adolescents with Autoimmune Thyroid Disease
Authors: Putarek Natasa Rojnic , Krnic Nevena , Knezevic-Cuca Jadranka , Kusec Vesna , Baretic Maja , Dumic Miroslav
Published: January 2022

The present study aims to investigate islet autoimmunity and susceptibility to type 1 diabetes (T1D) in children/adolescents with autoimmune thyroid disease (AITD), and family members of AITD patients with islet autoimmunity. Islet-cell cytoplasmic, glutamic-acid decarboxylase and tyrosine-phosphatase autoantibodies were measured in 161 AITD patients [(127 with autoimmune thyroiditis (AT); 34 with Graves’ disease (GD)], 20 family members of AITD patients with islet autoimmunity, and 155 age-matched controls. Islet autoimmunity was found in 10.6% of AITD patients, significantly more frequent than in controls (1.9%; p=0.002). Higher prevalence of islet autoantibodies was found in females with AITD (p=0.011) but not in males (p=0.16) as well as in AT (p=0.013) but not GD patients (p=0.19), compared to corresponding controls. Two or three islet autoantibodies were found concurrently in six AITD patients with islet autoimmunity. They all developed T1D and had significantly higher islet autoantibodies titers (p=0.01) compared to AITD patients with single islet autoantibodies but normal glucose metabolism. T1D was found in 3.7% of AITD patients compared to 0.2% in age-matched, general Croatian population. Islet autoantibodies were found in 5/20 family members of AITD patients with islet autoimmunity, among which two developed T1D. None of the controls was positive to more than one islet autoantibodies or developed T1D. Conclusion: Children/adolescents with AITD (particularly females and patients with AT) represent a risk group for islet autoimmunity and T1D, as well as family members of AITD patients with positive islet autoantibodies, but last observation must be examined in a larger number of patients.
Storage Method of Multi-Channel Lidar Data Based on Tree Structure
Authors: Chen Hao , Gao Fei , Zhu Qingsong , Yan Qing , Hua Dengxin , Stanič Samo
Published: January 2022

The multi-channel lidar has the characteristics of fast acquisition speed, large data volume, high dimension, and strong real-time storage, which makes it difficult to be met using the traditional lidar data storage methods. This paper presents a novel approach to store and convert the multi-channel lidar data by traversal method of the tree structure and binary code. In the proposed approach, a tree structure is constructed based on the multi-dimensional characteristics of multi-channel lidar data and the hierarchical relationship between them. The adjacency table storage structure data in the memory is used to generate the sub-tree of the multi-channel lidar data. The results show that the proposed tree structure approach can save the storage capacity and improve the retrieval speed, which can meet the needs of efficient storage and retrieval of multi-channel lidar data.
Critical Appraisal of Minimally Invasive Keyhole Surgery for Intracranial Meningioma
Authors: Thakur Jai Deep , Mallari Regin Jay , Corlin Alex , Yawitz Samantha , Eisenberg Amalia , Rhee John , Sivakumar Walavan , Krauss Howard , Martin Neil , Griffiths Chester , Barkhoudarian Garni , Kelly Daniel F.
Published: January 2022

Purpose

Minimally invasive meningioma removal through transcranial and endoscopic endonasal keyhole routes remain controversial. Herein we detail results of keyhole meningioma removal defined as use of a minimally invasive “retractorless” approach for which a traditional larger approach is often used instead.
Methods

Retrospective analysis from 2008-2021 of consecutive patients undergoing keyhole meningioma removal through one of six approaches: extent of resection, complications, endoscopy use, MRI FLAIR/T2 changes. Surgical goal was maximal safe removal including conservative(subtotal) removal for some invasive locations.
Results

Of 329 patients, keyhole approaches were utilized in 193(59%) patients (mean age 59±13; 30(15.5%) prior surgery) who underwent 213 operations; 205(96%) were skull base location. Approaches included: endoscopic endonasal(n=74,35%), supraorbital(n=73,34%), retromastoid(n=38,18%), mini-pterional(n=20,9%), suboccipital(n=4,2%), and contralateral transfalcine(n=4,2%). Primary outcomes: Gross total/near total(>90%) resection: 125(59%); (5% for petroclival, cavernous sinus/Meckel’s cave, spheno-cavernous locations vs 77% other locations). Complications included: permanent neurological worsening12(6%); CSF leak 2(1%); meningitis 2(1%); no patients sustained DVT, PE, MI, or 30-day mortality. Median LOS was 3 days with 94% discharged home and 96% with favorable 90-day KPS. Secondary outcomes: Small persistent FLAIR/T2 changes: 11(5.2%) patients. Endoscopy use: 87/139(63%) of craniotomies, facilitating additional tumor removal in 55%. Tumor progression was observed in 26(13%) patients(mean follow-up 42±36 months).
Conclusion

This analysis suggests keyhole meningioma removal can be associated with reasonable resection rates, low complication rates, short hospitalizations and high 90-day performance scores. Subtotal removal may be appropriate for invasive/adherent meningiomas to avoid complications. With careful patient selection and requisite experience, these approaches may be considered alternatives to traditional approaches.
Genital and Oral Microbiome and Behçet’s Disease Activity
Authors: Senusi Amal A. , Ogunkolade William , Sandionigi Anna , Fortune Farida
Published: January 2022

BackgroundThe aetiopathogeneses of Behçet’s Disease (BD) remains elusive with multifactorial genetic and epigenetic factors resulting in multisystemic disease. Oral and genital ulceration are common and influences disease outcome. We hypothesised that dysregulation of genital and oral microbial communities contributes to BD disease activity. 153 BD patients’ samples, 70 matched oral and genital (Female: Male, 58:12; mean age, 42±13.9: 39.3±10.3), 12 unmatched samples; 16s rRNA sequencing utilised and V1/V2 and V3/V4 regions analysed. BD outcomes: oral and genital ulcer severity and BD activity scores, Psychological and Social Well-being scales, Headache Impact Test-6 (HIT-6) were included. All the analyses were performed with R software. ResultsThe alpha and beta diversity had anatomical specificity, with significant differences between genital and oral samples; p values<0.05 irrespective of presence or absence of ulcers. Interestingly, in the genital area Bacteroidota were present (G_U: 29% - 10%) and (G_nU: 27% - 14%) compared to less than 1% oral area of V1/V2 and V3/V4. Proteobacteria were uniquely present with (O_U: 9%) and (O_nU: 12%) in oral, and less than 0.01% in genital area for V3/V4 region. Gender anatomical specific communities were noted: females with genital ulcers Gardnerella, Lactobacillus, Atopobium were significantly increased compared to than males, with V3/V4 analysis indicating that Lactobacillus and Gardnerella were significantly increased by 20 times in females than males (p-adj <0.05). In contrast Peptoniphilus and Corynebacterium were significantly increased in males than females. Streptococcus was significantly increased with oral ulceration, while Veillonella was significantly decreased in patients without oral ulceration. Colchicine had a significant effect on the bacterial abundance irrespective of the presence or absence of ulceration. In this cohort, the WSAS (Work and Social Adjustment Scale) values were higher in active disease. ConclusionOur results suggest that dysregulated microbial communities occur in BD. V1/V2 demonstrates that during episodes of ulceration the pathogenic bacteria genus Escherichia-Shigella appear in both oral and genital ulcers. V3/V4 outcomes show that ulceration in both regions is assigned to genus; Lachnospiraceae, Saccharimonidales, Coriobacteriales. Streptococcus is related to the presence of oral ulcers, while Veillonella is presence when patients are ulcers free may be a useful marker of disease regression.
Crossed Andreev Reflection in Zigzag Phosphorene Nanoribbon Based Ferromagnet/Superconductor/Ferromagnet Junctions
Authors: Li Ruigang , Chen Lei , Liu Jun-Feng , Wang Jun
Published: January 2022

We study the crossed Andreev reflection in zigzag phosphorene nanoribbon based ferromagnet/superconductor/ferromagnet junction. Only edge states, which are entirely detached from the bulk gap, involved in the transport process. The perfect crossed Andreev reflection, with the maximal nonlocal conductance −2e 2 /h, is addressed by setting the chemical potentials of the leads properly. At this situation, the local Andreev reflection and the electron tunneling are completely eliminated, the incoming electrons can only be reflected as electrons or transmitted as holes, corresponding to the electron reflection and the crossed Andreev reflection respectively. The nonlocal conductance oscillates periodically with the length and the chemical potential of the superconductor. Our study shows that the phosphorene based junction can be used as the quantum device to generate entangled-electrons.
A Stochastic Model for Kala-azar Transmission Dynamics in Libo Kemkem, Ethiopia
Authors: Alemu Sewmehon Shimekaw
Published: January 2022

The objective of this paper is to analyse and demonstrate the dynamics of Kala-azar infected group using stochastic model, particularly using simple SIR model with python script over time. The model is used under a closed population with N = 100, transmission rate coefficient β = 0.09, recovery rate γ = 0.03 and initial condition I(0) = 1. In the paper it is discussed how the Kala-azar infected group behaves through simple SIR model. The paper is completed with stochastic SIR model simulation result and shows stochasticity of the dynamics of Kala-azar infected population over time. Fig. 2 below depicts continuous fluctuations which tells us the disease evolves with stochastic nature and shows random process.Subject: Infectious Disease, Global Health, Health Informatics and Statistical and Computational Physics
Safety and Immunogenicity of Mix-Match of Vaccines -Covishield and Covaxin – a Pilot Study
Authors: Attunuru Bhanu Prakash Reddy , Reddy Podduturi Naveenchander , Mitnala Sasikala , Deepika Gujjarlapudi , Veturi Sadhana Yelamanchili , Reddy Duvvur Nageshwar
Published: January 2022

This single-center prospective observational study was conducted to assess the safety and immunogenicity of combination vaccines AstraZeneca’s ChAdOx1-nCov-19 (Covishield in India) and inactivated whole virion BBV152 (Covaxin). A total of 330 unvaccinated healthy volunteers were screened for SARS-COV2 seropositivity. RT PCR tests were conducted for seronegative volunteers (n =44). They were randomly assigned to four groups and given either same or mixed vaccines at an interval of 4 weeks between the two doses. Mix and match of vaccines did not evoke any adverse events. Combination of vaccines elicited similar immune responses in 4 groups. They were further studied dividing into homologous and heterologous vaccine groups. In Conclusion, Combination vaccines are safe and immunogenic and heterologous vaccines elicit better immunogenic response.
Scyphocephalione a Isolated from the Stem Bark of Scyphocephalium Ochocoa (Myristicaceae) Attenuate Acute and Chronic Pains Through the Antiinflammatory Activity
Authors: Mbiantcha Marius , Feuya Raymond Tchouya Guy , Yousseu William Nana , Atsamo Donatien Albert , Foundikou Hibrahim , Lebibi Jacques , Zemo Franklin Gamo
Published: January 2022

Abstract

In the treatment of cancer, patients that receive anti-cancer drugs such as Vincristine develop peripheral neuropathic pain. Scyphocephalione A is a new bioactive compound isolated from Scyphocephalium ochocoa (Myristicaceae), a medicinal plant traditionally used in African countries. Recently, an in vitro study has shown its anti-inflammatory and cytotoxic activities on MCF-7 cell line of mammary carcinoma. The purpose of the present study was to assess the in vitro anti-inflammatory and in vivo anti-nociceptive activities of Scyphocephalione A. In vitro tests were carried out on cyclooxygenase and 5-lipoxygenase activities, and on protein denaturation; while in vivo tests were performed on acute and chronic pain models. It was noticed that, Scyphocephalione A (1000 µg/ml), inhibits proteins denaturation, cyclooxygenase and 5-lipoxygenase activities respectively by 74.21%, 75.80% and 64.43%. The dose 50 mg/kg of Scyphocephalione A, inhibits acetic acid (63.43%, p<0.001) and formalin (42.12% (p<0.001) within first phase and 67.53% (p<0.001) within second phase)-induced pains. At the same dose, Scyphocephalione A significantly inhibited mechanical and heat hyperalgesia, as well as cold allodynia induced by vincristine. In addition, the compound restored haematological, biochemical and oxidative stress parameters which were altered following Vincristine administration. These results suggest that Scyphocephalione A is endowed with anti-inflammatory potential and antinociceptive properties. Therefore, Scyphocephalione A can be classified as a promising molecule for the management of peripheral neuropathic pain triggered by anti-cancer drug.
A Novel Approach to Charcoal Fine Waste: Sustainable Use as Filling of Polymeric Matrices
Authors: Delatorre Fabíola Martins , Cupertino Gabriela Fontes Mayrinck , Oliveira Michel Picanço , Gomes Felipe Silva , Profeti Luciene Paula Roberto , Profeti Demetrius , Júnior Mário Guimarães , Azevedo Márcia Giardinieri , Saloni Daniel , Dias Ananias Francisco
Published: January 2022

This study aims to evaluate charcoal fines as potential reinforcing agents in biocomposites. Charcoal has both high carbon content and surface area depending on the manufacturing temperatures. Charcoal is a common residue in the coal industry that we propose using it to reinforce filling agents in several matrices in order to add value to this residue. This study investigated charcoal fines when using three pyrolysis temperatures (400, 600, and 800°C) to identify the most suitable charcoal to be used as raw materials in producing carbon biocomposites. We evaluated apparent density, porosity, morphology, and immediate chemical composition, and then performed a Fourier-transform infrared spectroscopy (FTIR) and an X-ray photoelectron spectroscopy (XPS). Charcoal fines produced at 800°C showed promising results as a polymeric matrix filling due to their higher porosity (81.08%), fixed carbon content (96.77%), and hydrophobicity.
Health-Related Quality Of Life And Clinical Outcome After Radiotherapy of Patients With Intracranial Meningioma
Authors: Lisowski Dominik , Trömel Jannik , Lutyj Paul , Lewitzki Victor , Hartrampf Philipp E. , Polat Bülent , Flentje Michael , Tamihardja Jörg
Published: January 2022

Purpose: This retrospective, single-institutional study investigated long-term outcome, toxicity and health-related quality of life (HRQOL) in meningioma patients after radiotherapy. Methods: We analyzed the data of 119 patients who received radiotherapy at our department from 1997 to 2014 for intracranial WHO grade I-III meningioma. Fractionated stereotactic radiotherapy (FSRT), intensity modulated radiotherapy (IMRT) or radiosurgery radiation was applied using a linear accelerator. A median total dose of 54.7 Gy (IQR 54.0-60.0 Gy) was prescribed in median single doses of 1.8 Gy (IQR 1.8-1.9 Gy). Overall survival (OS) and local control (LC) rates were obtained. In addition, the European Organisation for Research and Treatment of Cancer (EORTC) Quality of Life Core questionnaire (QLQ-C30) and the EORTC Brain Cancer Module (QLQ-BN20) were completed for assessment of HRQOL. Results were compared with normal population and with already published data. Results: OS for the entire cohort was 89.6% at 5 years and 75.9% at 10 years. LC at 5 and 10 years was 82.4% and 73.4%, respectively. Local recurrence was observed in 22 patients (18.5%). CTCAE grade ≥3 acute and chronic toxicities were observed in seven patients (5.9%) and five patients (4.2%), respectively. Global health status was rated with a mean of 59.9 points (SD 22.3) on QLQ-C30. HRQOL was lower after radiotherapy in comparison to normal population and to meningioma patients treated by surgery alone. Conclusion: Radiotherapy resulted in very good long-term survival and tumor control rates with low rates of severe toxicities. Nevertheless, long-term HRQOL may be negatively affected by radiotherapy.
Longitudinal Follow-Up on the Growth Rates and Influence Factors of Preterm and Full-Term Infants in Urban Areas of Hubei Province: A Birth Cohort Study
Authors: Xiong Zhonggui , Sun Feimin , Xia Zeyuan
Published: January 2022

Objective: It was to explore the growth rates and influence factors of length and weight of preterm and full-term infants in urban areas of Hubei province to provide reference for child health and related clinical field.Methods: A birth cohort study was used to analyze the growth rates and influence factors of length and weight of preterm and full-term infants in urban areas of Hubei province by multivariate regression analysis.Results: The growth rates of length and weight of preterm infants were significantly lower than those of full-term infants from birth to 3 months (P < 0.05), and gradually caught up to the level of full-term infants after 3 months (P > 0.05). Meanwhile, there were some influence factors which had significant differences by the contribution to the growth rates of length and weight of preterm and full-term infants. It was proposed that the perinatal factors were attached more importance to the growth rates of preterm infants.Conclusion: This study was to investigate the growth rates and influence factors of length and weight of preterm and full-term infants, and to take effective intervention measures to promote growth and development of preterm infants in urban areas of Hubei province.