bluuebunny/crossref_metadata_2025 huggingface dataset:
QTL for Stress and Disease Resistance in European Sea Bass, Dicentrarhus labrax L.
Authors: Chatziplis Dimitrios , Oikonomou Stavroula , Loukovitis Dimitrios , Tsiokos Dimitrios , Samaras Athanasios , Dimitroglou Arkadios , Kottaras Lefteris , Papanna Kantham , Papaharisis Leonidas , Tsigenopoulos Costas , Pavlidis Michail
Published: September 2020

There is a growing interest in selective breeding in European sea bass (Dicentrarchus labrax), especially regarding family selection based on growth performance. In particular, quantitative trait loci (QTL) identification in sea bass enhances the application of marker-assisted breeding for the genetic improvement of the production traits. The aims of the study were to identify potential QTL affecting stress and immunological indicators, body weight, and mortality after vibriosis injection in sea bass as well as to estimate heritability and genetic/phenotypic correlations for the aforementioned traits. To this end, stress test was performed on 960 offspring and a sub-group of them (420) was selected to explore the mortality after vibrio injection. Selective genotyping was performed in 620 offspring for 35 microsatellite markers and distributed into 6 linkage groups. The length of the genetic linkage map was 283.6 cM and the mean distance between the markers was 8.1 cM. QTL affecting body weight in three different growth periods detected on linkage groups LG1, LG4, LG6, and LG14. A QTL associated with weight in early growth stages (290–306 days post-hatching) was also identified on LG3. QTL analysis confirmed the existence of QTL affecting cortisol levels, on LG3 and LG14. Moreover, new QTL affecting only cortisol and glucose levels were detected on LG1 and LG23. No QTL affecting hormonal or biochemical marks was found on LG4 and LG6. Heritability of cortisol, lysozyme levels, and mortality were high (0.36, 0.55, and 0.38, respectively).
Host Diversity and Origin of Zoonoses: The Ancient and the New
Authors: Recht Judith , Schuenemann Verena J. , Sánchez-Villagra Marcelo R.
Published: September 2020

Bacterial, viral, and parasitic zoonotic diseases are transmitted to humans from a wide variety of animal species that act as reservoir hosts for the causative organisms. Zoonoses contribute an estimated 75% of new or reemerging infectious diseases in humans. All groups of mammals have been shown to act as hosts for transmission of different organisms that cause zoonoses, followed in importance by birds; with both wild and domestic species identified as hosts in specific cases. There has been considerable research progress leading to a better understanding of the host range, animal origin, evolution, and transmission of important zoonoses, including those caused by the ingestion of food and products derived from animals. Paleopathology studies of ancient human bone lesions, in combination with ancient DNA analysis of the causative pathogen, have contributed to our understanding of the origin of zoonotic diseases, including brucellosis and mycobacterial zoonoses. However, there are still knowledge gaps and new confirmed and potential hosts are reported locally with some frequency. Both the economic cost and burden of disease of zoonoses are substantial at local and global levels, as reflected by recent coronavirus pandemics that spread rapidly around the world. Evidence-based prevention strategies are currently a global priority increasingly recognized, especially in zoonoses-affected regions.
Transdermal Fentanyl Uptake at Two Different Patch Locations in Swiss White Alpine Sheep
Authors: Buchholz Tim , Hildebrand Maria , Heider Anja , Stenger Valentina , Arens Daniel , Spadavecchia Claudia , Zeiter Stephan
Published: September 2020

When using animals in biomedical research, investigators have the responsibility to ensure adequate analgesia. Currently, transdermal fentanyl patches (TFP) are often used to provide postoperative analgesia in large laboratory animals. The aim of this study was to compare the fentanyl uptake resulting from TFP applied at two different locations, namely the foreleg and the thorax, in healthy adult sheep. Twelve sheep received a TFP with an intended dosage of 2 ug/kg/h. Blood samples were taken at different time points over a period of five days and the fentanyl plasma levels were measured. The TFP applied on the foreleg allowed a faster fentanyl uptake with higher peaks and a longer time within or above the target concentration of 0.6–1.5 ng/mL, shown to be analgesic in humans, when compared to the one on the thorax. Assuming that the effective plasma concentration described for humans is providing analgesia in sheep as well, the present findings suggest that it should be sufficient to apply the TFP 3–6 h before the painful insult and that its effect should last at least 48 h. Furthermore, when TFP are used to provide postoperative analgesia in sheep, they should be placed on the foreleg rather than on the thorax.
Critical Perspective of Animal Production Specialists on Cell-Based Meat in Brazil: From Bottleneck to Best Scenarios
Authors: Heidemann Marina S. , Taconeli Cesar A. , Reis Germano G. , Parisi Giuliana , Molento Carla F. M.
Published: September 2020

Recently, many studies regarding consumer perception of cell-based meat have been published. However, the opinion of the professionals involved in animal production also seems relevant. In particular, veterinarians and animal scientists may be important players in the new cell-based meat production, acting as proponents or barriers to this major improvement for farm animal welfare. Therefore, our aim is to analyse the knowledge and perspective of Brazilian veterinarians and animal scientists regarding cell-based meat. Veterinarians (76.8%; 209/272) and animal scientists (23.2%; 63/272) responded to an online survey. Logistic regression, latent class and logit models were used to evaluate objective answers, and the Discourse of the Collective Subject method was used to interpret open-ended answers. Specialists who were women (62.5%; 170/272), veterinarians (76.8%; 209/272), vegetarians (7.0%; 19/272) and vegans (1.1%; 3/272) were more supportive of cell-based meat. Lack of knowledge and the connection with artificiality, the most frequent spontaneous word associated with cell-based meat by all respondents, were the main negative points highlighted. Thus, it seems fundamental to offer higher education to veterinarians and animal scientists regarding cell-based meat, since engaging them with this novel technology may mitigate both the resistance and its negative consequences for the professionals, society, the animals involved and the environment.
Suppression of Oxidative Stress as Potential Therapeutic Approach for Normal Tension Glaucoma
Authors: Harada Chikako , Noro Takahiko , Kimura Atsuko , Guo Xiaoli , Namekata Kazuhiko , Nakano Tadashi , Harada Takayuki
Published: September 2020

Glaucoma is a neurodegenerative disease of the eye, which involves degeneration of retinal ganglion cells (RGCs): the output neurons of the retina to the brain, which with their axons comprise the optic nerve. Recent studies have shown the possible involvement of oxidative stress in the pathogenesis of glaucoma, especially in the subtype of normal tension glaucoma. Basic experiments utilizing rodent and primate models of glaucoma revealed that antioxidants protect RGCs under various pathological conditions including glutamate neurotoxicity and optic nerve injury. These results suggested that existing drugs and food factors may be useful for prevention and hence therapy of glaucoma. In this review, we highlight some therapeutic candidates, particularly those with antioxidant properties, and discuss the therapeutic potential of RGC protection by modulating gene expressions that prevent and ameliorate glaucoma.
Phototherapy as a Rational Antioxidant Treatment Modality in COVID-19 Management; New Concept and Strategic Approach: Critical Review
Authors: Hanna Reem , Dalvi Snehal , Sălăgean Tudor , Bordea Ioana Roxana , Benedicenti Stefano
Published: September 2020

The COVID-19 pandemic has taken the entire globe by storm. The pathogenesis of this virus has shown a cytokine storm release, which contributes to critical or severe multi-organ failure. Currently the ultimate treatment is palliative; however, many modalities have been introduced with effective or minimal outcomes. Meanwhile, enormous efforts are ongoing to produce safe vaccines and therapies. Phototherapy has a wide range of clinical applications against various maladies. This necessitates the exploration of the role of phototherapy, if any, for COVID-19. This critical review was conducted to understand COVID-19 disease and highlights the prevailing facts that link phototherapy utilisation as a potential treatment modality for SARS-CoV-2 viral infection. The results demonstrated phototherapy’s efficacy in regulating cytokines and inflammatory mediators, increasing angiogenesis and enhancing healing in chronic pulmonary inflammatory diseases. In conclusion, this review answered the following research question. Which molecular and cellular mechanisms of action of phototherapy have demonstrated great potential in enhancing the immune response and reducing host–viral interaction in COVID-19 patients? Therefore, phototherapy is a promising treatment modality, which needs to be validated further for COVID-19 by robust and rigorous randomised, double blind, placebo-controlled, clinical trials to evaluate its impartial outcomes and safety.
Combined Treatment with Low Cytotoxic Ethyl Acetate Nepenthes Extract and Ultraviolet-C Improves Antiproliferation to Oral Cancer Cells via Oxidative Stress
Authors: Peng Sheng-Yao , Lin Li-Ching , Yang Zhe-Wei , Chang Fang-Rong , Cheng Yuan-Bin , Tang Jen-Yang , Chang Hsueh-Wei
Published: September 2020

Ultraviolet-C (UVC) irradiation provides an alternative radiotherapy to X-ray. UVC sensitizer from natural products may improve radiotherapy at low cytotoxic side effects. The aim of this study is to assess the regulation for oral cancer cell proliferation by a combined treatment of UVC and our previously reported anti-oral cancer natural product (ethyl acetate extract of Nepenthes adrianii × clipeata; EANA). The detailed possible UVC sensitizing mechanisms of EANA such as effects on cell proliferation, cell cycle, apoptosis, and DNA damage are investigated individually and in combination using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTS) assay, flow cytometry, and western blotting at low dose conditions. In a 24 h MTS assay, the low dose EANA (5 μg/mL) and low dose UVC (12 J/m2) individually show 80% and combinedly 57% cell proliferation in oral cancer Ca9-22 cells; but no cytotoxicity to normal oral HGF-1 cells. Mechanistically, low dose EANA and low dose UVC individually induce apoptosis (subG1 accumulation, pancaspase activation, and caspases 3, 8, 9), oxidative stress (reactive oxygen species, mitochondrial superoxide, and mitochondrial membrane potential depletion), and DNA damage (γH2AX and 8-hydroxy-2′-deoxyguanosine). Moreover, the combined treatment (UVC/EANA) synergistically induces these changes. Combined low dose treatment-induced antiproliferation, apoptosis, oxidative stress, and DNA damage were suppressed by the ROS scavenger N-acetylcysteine. In conclusion, UVC/EANA shows synergistic antiproliferation, oxidative stress, apoptosis, and DNA damage to oral cancer cells in an oxidative stress-dependent manner. With the selective killing properties of low dose EANA and low dose UVC, EANA provides a novel UVC sensitizing agent to improve the anti-oral cancer therapy.
N-Acetylaspartylglutamate (NAAG) Pretreatment Reduces Hypoxic-Ischemic Brain Damage and Oxidative Stress in Neonatal Rats
Authors: Bratek Ewelina , Ziembowicz Apolonia , Salinska Elzbieta
Published: September 2020

N-acetylaspartylglutamate (NAAG), the most abundant peptide transmitter in the mammalian nervous system, activates mGluR3 at presynaptic sites, inhibiting the release of glutamate, and acts on mGluR3 on astrocytes, stimulating the release of neuroprotective growth factors (TGF-β). NAAG can also affect N-methyl-d-aspartate (NMDA) receptors in both synaptic and extrasynaptic regions. NAAG reduces neurodegeneration in a neonatal rat model of hypoxia-ischemia (HI), although the exact mechanism is not fully recognized. In the present study, the effect of NAAG application 24 or 1 h before experimental birth asphyxia on oxidative stress markers and the potential mechanisms of neuroprotection on 7-day old rats was investigated. The intraperitoneal application of NAAG at either time point before HI significantly reduced the weight deficit of the ischemic brain hemisphere, radical oxygen species (ROS) content and activity of antioxidant enzymes, and increased the concentration of reduced glutathione (GSH). No additional increase in the TGF-β concentration was observed after NAAG application. The fast metabolism of NAAG and the decrease in TGF-β concentration that resulted from NAAG pretreatment, performed up to 24 h before HI, excluded the involvement mGluR3 in neuroprotection. The observed effect may be explained by the activation of NMDA receptors induced by NAAG pretreatment 24 h before HI. Inhibition of the NAAG effect by memantine supports this conclusion. NAAG preconditioning 1 h before HI results in a mixture of mGluR3 and NMDA receptor activation. Preconditioning with NAAG induces the antioxidative defense system triggered by mild excitotoxicity in neurons. Moreover, this response to NAAG pretreatment is consistent with the commonly accepted mechanism of preconditioning. However, this theory requires further investigation.
Antioxidant Profile of Pepper (Capsicum annuum L.) Fruits Containing Diverse Levels of Capsaicinoids
Authors: Palma José M. , Terán Fátima , Contreras-Ruiz Alba , Rodríguez-Ruiz Marta , Corpas Francisco J.
Published: September 2020

Capsicum is the genus where a number of species and varieties have pungent features due to the exclusive content of capsaicinoids such as capsaicin and dihydrocapsaicin. In this work, the main enzymatic and non-enzymatic systems in pepper fruits from four varieties with different pungent capacity have been investigated at two ripening stages. Thus, a sweet pepper variety (Melchor) from California-type fruits and three autochthonous Spanish varieties which have different pungency levels were used, including Piquillo, Padrón and Alegría riojana. The capsaicinoids contents were determined in the pericarp and placenta from fruits, showing that these phenyl-propanoids were mainly localized in placenta. The activity profiles of catalase, total and isoenzymatic superoxide dismutase (SOD), the enzymes of the ascorbate–glutathione cycle (AGC) and four NADP-dehydrogenases indicate that some interaction with capsaicinoid metabolism seems to occur. Among the results obtained on enzymatic antioxidants, the role of Fe-SOD and the glutathione reductase from the AGC is highlighted. Additionally, it was found that ascorbate and glutathione contents were higher in those pepper fruits which displayed the greater contents of capsaicinoids. Taken together, all these data indicate that antioxidants may contribute to preserve capsaicinoids metabolism to maintain their functionality in a framework where NADPH is perhaps playing an essential role.
Vitamin C and E Treatment Blunts Sprint Interval Training–Induced Changes in Inflammatory Mediator-, Calcium-, and Mitochondria-Related Signaling in Recreationally Active Elderly Humans
Authors: Wyckelsma Victoria L. , Venckunas Tomas , Brazaitis Marius , Gastaldello Stefano , Snieckus Audrius , Eimantas Nerijus , Baranauskiene Neringa , Subocius Andrejus , Skurvydas Albertas , Pääsuke Mati , Gapeyeva Helena , Kaasik Priit , Pääsuke Reedik , Jürimäe Jaak , Graf Brigitte A. , Kayser Bengt , Place Nicolas , Andersson Daniel C. , Kamandulis Sigitas , Westerblad Håkan
Published: September 2020

Sprint interval training (SIT) has emerged as a time-efficient training regimen for young individuals. Here, we studied whether SIT is effective also in elderly individuals and whether the training response was affected by treatment with the antioxidants vitamin C and E. Recreationally active elderly (mean age 65) men received either vitamin C (1 g/day) and vitamin E (235 mg/day) or placebo. Training consisted of nine SIT sessions (three sessions/week for three weeks of 4-6 repetitions of 30-s all-out cycling sprints) interposed by 4 min rest. Vastus lateralis muscle biopsies were taken before, 1 h after, and 24 h after the first and last SIT sessions. At the end of the three weeks of training, SIT-induced changes in relative mRNA expression of reactive oxygen/nitrogen species (ROS)- and mitochondria-related proteins, inflammatory mediators, and the sarcoplasmic reticulum Ca2+ channel, the ryanodine receptor 1 (RyR1), were blunted in the vitamin treated group. Western blots frequently showed a major (>50%) decrease in the full-length expression of RyR1 24 h after SIT sessions; in the trained state, vitamin treatment seemed to provide protection against this severe RyR1 modification. Power at exhaustion during an incremental cycling test was increased by ~5% at the end of the training period, whereas maximal oxygen uptake remained unchanged; vitamin treatment did not affect these measures. In conclusion, treatment with the antioxidants vitamin C and E blunts SIT-induced cellular signaling in skeletal muscle of elderly individuals, while the present training regimen was too short or too intense for the changes in signaling to be translated into a clear-cut change in physical performance.
Satellite InSAR as a New Tool for the Verification of Landslide Engineering Remedial Works at the Regional Scale: A Case Study in the Three Gorges Resevoir Area, China
Authors: Liu Shuhao , Segoni Samuele , Raspini Federico , Yin Kunlong , Zhou Chao , Zhang Yiyue , Casagli Nicola
Published: September 2020

Several countries worldwide are funding large-scale programs to mitigate landslide risk by implementing engineering remedial works. However, the overall effectiveness of such measures is rarely monitored, and they are typically performed at the slope scale without fully exploiting the wide-area capabilities of remote sensing technologies. A multi-scale and multi-source monitoring procedure for evaluating the slope stability and the effectiveness of related remedial works was proposed in this study and applied in the middle section of the Three Gorges Reservoir Area (TGRA), China. The area is highly exposed to landslide hazards, and a massive program of engineering remedial works was recently implemented. Satellite interferometric synthetic aperture radar (InSAR)-based techniques were first exploited at the regional scale with the objective to provide a general overview of the deformative scenario and to highlight localized problems (active landslides or high deformation zones) to be further investigated; then, local-scale field investigation and multi-source ground monitoring data were employed to verify the deforming states of active landslides and to evaluate the effectiveness of the landslide engineering remedial works. The results indicated that, among the 310 mapped landslides in the study area, 52 were identified to be active and in a slow-moving state by satellite InSAR; Among the 58 controlled landslides, 9 of them were suspected to be active in a slow-moving state and require further concern. Particular attention was paid to two controlled landslides that were found in a continuously and progressively deforming state. We observed that the regional-scale program of slope stabilization was highly successful; however, the variation of the surrounding environmental setting could have led to landslide reactivation or partial invalidation of the landslide remedial works. The proposed multi-scale and multi-source monitoring framework is low-cost, easy to perform, and very straightforward to communicate to citizens and authorities. It can be easily implemented with very wide areas to assess the slope stability and to investigate the effectiveness of large-scale governmental risk mitigation programs, identifying precursor signals that could allow for intervention before reaching critical conditions.
Fi-Fo Detector: Figure and Formula Detection Using Deformable Networks
Authors: Younas Junaid , Siddiqui Shoaib Ahmed , Munir Mohsin , Malik Muhammad Imran , Shafait Faisal , Lukowicz Paul , Ahmed Sheraz
Published: September 2020

We propose a novel hybrid approach that fuses traditional computer vision techniques with deep learning models to detect figures and formulas from document images. The proposed approach first fuses the different computer vision based image representations, i.e., color transform, connected component analysis, and distance transform, termed as Fi-Fo image representation. The Fi-Fo image representation is then fed to deep models for further refined representation-learning for detecting figures and formulas from document images. The proposed approach is evaluated on a publicly available ICDAR-2017 Page Object Detection (POD) dataset and its corrected version. It produces the state-of-the-art results for formula and figure detection in document images with an f1-score of 0.954 and 0.922, respectively. Ablation study results reveal that the Fi-Fo image representation helps in achieving superior performance in comparison to raw image representation. Results also establish that the hybrid approach helps deep models to learn more discriminating and refined features.
Analysis of the Thermomechanical Fatigue Behavior of Fully Ferritic High Chromium Steel Crofer®22 H with Cyclic Indentation Testing
Authors: Blinn Bastian , Görzen David , Fischer Torsten , Kuhn Bernd , Beck Tilmann
Published: September 2020

The 22 wt.% Cr, fully ferritic stainless steel Crofer®22 H has higher thermomechanical fatigue (TMF)- lifetime compared to advanced ferritic-martensitic P91, which is assumed to be caused by different damage tolerance, leading to differences in crack propagation and failure mechanisms. To analyze this, instrumented cyclic indentation tests (CITs) were used because the material’s cyclic hardening potential—which strongly correlates with damage tolerance, can be determined by analyzing the deformation behavior in CITs. In the presented work, CITs were performed for both materials at specimens loaded for different numbers of TMF-cycles. These investigations show higher damage tolerance for Crofer®22 H and demonstrate changes in damage tolerance during TMF-loading for both materials, which correlates with the cyclic deformation behavior observed in TMF-tests. Furthermore, the results obtained at Crofer®22 H indicate an increase of damage tolerance in the second half of TMF-lifetime, which cannot be observed for P91. Moreover, CITs were performed at Crofer®22 H in the vicinity of a fatigue crack, enabling to locally analyze the damage tolerance. These CITs show differences between crack edges and the crack tip. Conclusively, the presented results demonstrate that CITs can be utilized to analyze TMF-induced changes in damage tolerance.
Torrefaction of Coffee Husk Flour for the Development of Injection-Molded Green Composite Pieces of Polylactide with High Sustainability
Authors: Ortiz-Barajas Diana L. , Arévalo-Prada Johan A. , Fenollar Octavio , Rueda-Ordóñez Yesid J. , Torres-Giner Sergio
Published: September 2020

Coffee husk, a major lignocellulosic waste derived from the coffee industry, was first ground into flour of fine particles of approximately 90 µm and then torrefied at 250 °C to make it more thermally stable and compatible with biopolymers. The resultant torrefied coffee husk flour (TCHF) was thereafter melt-compounded with polylactide (PLA) in contents from 20 to 50 wt% and the extruded green composite pellets were shaped by injection molding into pieces and characterized. Although the incorporation of TCHF reduced the ductility and toughness of PLA, filler contents of 20 wt% successfully yielded pieces with balanced mechanical properties in both tensile and flexural conditions and improved hardness. Contents of up to 30 wt% of TCHF also induced a nucleating effect that favored the formation of crystals of PLA, whereas the thermal degradation of the biopolyester was delayed by more than 7 °C. Furthermore, the PLA/TCHF pieces showed higher thermomechanical resistance and their softening point increased up to nearly 60 °C. Therefore, highly sustainable pieces were developed through the valorization of large amounts of coffee waste subjected to torrefaction. In the Circular Bioeconomy framework, these novel green composites can be used in the design of compostable rigid packaging and food contact disposables.
Deformation Characteristics of the Shear Zone and Movement of Block Stones in Soil–Rock Mixtures Based on Large-Sized Shear Test
Authors: Li Zhiqing , Hu Feng , Qi Shengwen , Hu Ruilin , Zhou Yingxin , Bai Yawei
Published: September 2020

Soil–rock mixtures (SRM) have the characteristics of distinct heterogeneity and an obvious structural effect, which make their physical and mechanical properties very complex. This study aimed to investigate the deformation properties and failure mode of the shear zone as well as the movement of block stones in SRM experimentally, not only considering SRM shear strength. The particle composition and proportion of specimens were based on field samples from an SRM slope along national highway 318 in Xigaze, Tibet. Shear zone deformation tests were carried out using an SRM-1000 large-sized geotechnical apparatus controlled by a motor servo, considering the effects of different stone contents by mass (0, 30%, 50%, 70%), vertical pressures (50, 100, 200, 300, and 400 kPa), and block stone sizes (9.5–19.0, 19.0–31.5, and 31.5–53.0 mm). The characteristics of the shear zone deformation and block stone interactions were monitored by placing aluminum wires and dry ash in holes in the specimens. The results showed that the stone content 30% and 70% were two critical thresholds to determine the deformation characteristics of SRM. Under the conditions of high stone content and large particle size, the stones throughout the shear surface tended to extrude and roll during the shear process. The block stones around the shear surface were mainly affected by dilatancy and exhibited extrusion, particle breakage, and redistribution. The deformation pattern could be considered as be analogous to push-type shear deformation from the back to front or composite shear deformation from the front and back to the middle of the slope. It is of great importance to study the shear characteristics and deformation evolution of SRM to understand the progressive shear process of the sliding zone and the failure mode of landslides.
Dental Condition as A Factor Modifying the Transmission of the Sound Vibration in the Skull Bones
Authors: Balinski Slawomir , Morawska-Kochman Monika , Bolejko Romuald , Dudek Krzysztof , Bochnia Marek
Published: September 2020

Dental deficiencies coexist with hearing loss, and dental treatment can improve hearing acuity. To prove that different dentition affects the transmission of acoustic vibrations through bone conduction, we prepared six dry human skulls to reconstruct teeth and soft tissues. We measured the transmission of vibrations from the maxilla to the cochlea, in the toothless jaw (TJ), jaw with lateral defects with frame dentures (FD), toothless jaw with complete dentures (CD), and jaw with reconstructed dentition (RD). Each skull was flexibly suspended. The maxilla was stimulated with the bone vibrator Radioear B71. The vibrations of the pyramid were measured perpendicularly using the Polytec PSV-400-M2 scanning vibrometer. Characteristics of frequencies differed simultaneously on the left (l) and right (r) side of each skull. In all states (from 234 Hz to 5 kHz), we identified 10–21 resonant (R) and 9–21 antiresonant (AR) frequencies unilaterally (+/− 5%). In about 30% of cases, they were each time inconsistent with the “physiological” state-RD. In the 500 Hz–2 kHz frequency range (necessary for understanding speech), the effective vibrations velocities vRMS (mm/s) near cochlea were significantly lower in RD than in tree states, where (depending on the dentures) the least energy reached cochlea in FD and the most in TJ.
Sugarcane Industry Waste Recovery: A Case Study Using Thermochemical Conversion Technologies to Increase Sustainability
Authors: Nunes Leonel J. R. , Loureiro Liliana M. E. F. , Sá Letícia C. R. , Silva Hugo F. C.
Published: September 2020

The sugarcane industry has assumed an increasingly important role at a global level, with countries such as Brazil and India dominating the field. However, this causes environmental problems, since the industry produces large amounts of waste, such as sugarcane bagasse. This by-product, which is energetically partially recovered in sugar mills and in the pulp and paper industry, can make a significant contribution to the general use of biomass energy, if the usual disadvantages associated with products with low density and a high moisture content are overcome. From this perspective, thermochemical conversion technologies, especially torrefaction, are assumed to be capable of improving the fuel properties of this material, making it more appealing for potential export and use in far-off destinations. In this work, sugarcane samples were acquired, and the process of obtaining bagasse was simulated. Subsequently, the bagasse was dried and heat-treated at 200 and 300 °C to simulate the over-drying and torrefaction process. Afterward, product characterization was performed, including thermogravimetric analysis, elemental analysis, calorimetry, and energy densification. The results showed significant improvements in the energy content, from 18.17 to 33.36 MJ·kg−1 from dried bagasse to torrefied bagasse at 300 °C, showing that despite high mass loss, there is potential for a future value added chain for this waste form, since the increment in energy density could enhance its transportation and use in locations far off the production site.
A BIM-Based Value for Money Assessment in Public-Private Partnership: An Overall Review
Authors: Ren Guoqian , Li Haijiang , Zhang Jisong
Published: September 2020

Public-private partnerships (PPPs) have proliferated and adapted to public development in recent decades; within it, the value for money (VfM) assessment defines the feasibility of the project procurement model as one of the essential components of PPP. However, evaluating the VfM in PPPs remains problematic. Given concerns about PPP profitability, a more integrated VfM evaluation is urgently needed to manage multiple indicators along the project lifecycle. Building information management (BIM), popular in architecture, engineering, and construction, provides resources that could support the VfM to a great extent. This paper uses a review approach to identify the current issues that are affecting VfM assessments and suggests that BIM, functioning throughout the PPP lifecycle, could support decision-making in VfM processes in order to satisfy service targets.
Biomechanical Effect of Various Tibial Bearing Materials in Uni-Compartmental Knee Arthroplasty Using Finite Element Analysis
Authors: Kwak Yoon Hae , Hong Hyoung-Taek , Koh Yong-Gon , Kang Kyoung-Tak
Published: September 2020

This paper aimed to evaluate the biomechanical effects of tibial bearing materials, from ultra-high-molecular-weight polyethylene (UHMWPE) to poly-ether-ether-ketone (PEEK) and carbon-fiber-reinforced poly-ether-ether-ketone (CFR-PEEK). The studies were conducted based on a validated finite element model. The geometry of the intact knee model was developed from computed tomography and magnetic resonance imaging of the left knee joint of a 37-year-old healthy male volunteer. Three different loading conditions, related to the loads applied in the experimental research, were applied to this study for model predictions and validation. The contact stress in the other compartments was under normal walking conditions. Also, stresses on five regions of the tibia bone were analyzed under normal walking conditions. The lowest contact stress between the lateral meniscus and tibial cartilage was achieved in the order of the use of CFR-PEEK, PEEK, and UHMWPE tibial bearings. Moreover, CFR-PEEK and PEEK tibial bearings indicated lower and greater stresses on cortical and trabecular bones, respectively, compared to the UHMWPE tibial bearing. These results show that CFR-PEEK can be used as a tibial bearing material as an alternative to UHMWPE, and such a change in the material may be a good method for reducing potential anteromedial pain.
Socio-Environmental Dynamics of Alpine Grasslands, Steppes and Meadows of the Qinghai–Tibetan Plateau, China: A Commentary
Authors: Feng Haiying , Squires Victor R.
Published: September 2020

Alpine grasslands are a common feature on the extensive (2.6 million km2) Qinghai–Tibet plateau in western and southwestern China. These grasslands are characterized by their ability to thrive at high altitudes and in areas with short growing seasons and low humidity. Alpine steppe and alpine meadow are the principal plant Formations supporting a rich species mix of grass and forb species, many of them endemic. Alpine grasslands are the mainstay of pastoralism where yaks and hardy Tibetan sheep and Bactrian camels are the favored livestock in the cold arid region. It is not only their importance to local semi nomadic herders, but their role as headwaters of nine major rivers that provide water to more than one billion people in China and in neighboring countries in south and south-east Asia and beyond. Grasslands in this region were heavily utilized in recent decades and are facing accelerated land degradation. Government and herder responses, although quite different, are being implemented as climate change and the transition to the market economy proceeds apace. Problems and prospects for alpine grasslands and the management regimes being imposed (including sedentarization, resettlement and global warming are briefly discussed.
A Poplar Rust Effector Protein Associates with Protein Disulfide Isomerase and Enhances Plant Susceptibility
Authors: Madina Mst Hur , Rahman Md Saifur , Huang Xiaoqiang , Zhang Yang , Zheng Huanquan , Germain Hugo
Published: September 2020

Melampsora larici-populina (Mlp), the causal agent of Populus leaf rust, secretes an array of effectors into the host through the haustorium to gain nutrients and suppress immunity. The precise mechanisms by which these effectors promote virulence remain unclear. To address this question, we developed a transgenic Arabidopsis line expressing a candidate effector, Mlp124357. Constitutive expression of the effector increased plant susceptibility to pathogens. A GxxxG motif present in Mlp124357 is required for its subcellular localization at the vacuolar membrane of the plant cell, as replacement of the glycine residues with alanines led to the delocalization of Mlp124357 to the nucleus and cytoplasm. We used immunoprecipitation and mass spectrometry (MS) to identify Mlp124357 interaction partners. Only one of the putative interaction partners knock-out line caused delocalization of the effector, indicating that Arabidopsis protein disulfide isomerase-11 (AtPDI-11) is required for the effector localization. This interaction was further confirmed by a complementation test, a yeast-two hybrid assay and a molecular modeling experiment. Moreover, localization results and infection assays suggest that AtPDI-11 act as a helper for Mlp124357. In summary, our findings established that one of Mlp effectors resides at the vacuole surface and modulates plant susceptibility.
The Receptor Tyrosine Kinase TrkA Is Increased and Targetable in HER2-Positive Breast Cancer
Authors: Griffin Nathan , Marsland Mark , Roselli Severine , Oldmeadow Christopher , Attia John , Walker Marjorie M. , Hondermarck Hubert , Faulkner Sam
Published: September 2020

The tyrosine kinase receptor A (NTRK1/TrkA) is increasingly regarded as a therapeutic target in oncology. In breast cancer, TrkA contributes to metastasis but the clinicopathological significance remains unclear. In this study, TrkA expression was assessed via immunohistochemistry of 158 invasive ductal carcinomas (IDC), 158 invasive lobular carcinomas (ILC) and 50 ductal carcinomas in situ (DCIS). TrkA was expressed in cancer epithelial and myoepithelial cells, with higher levels of TrkA positively associated with IDC (39% of cases) (p < 0.0001). Interestingly, TrkA was significantly increased in tumours expressing the human epidermal growth factor receptor-2 (HER2), with expression in 49% of HER2-positive compared to 25% of HER2-negative tumours (p = 0.0027). A panel of breast cancer cells were used to confirm TrkA protein expression, demonstrating higher levels of TrkA (total and phosphorylated) in HER2-positive cell lines. Functional investigations using four different HER2-positive breast cancer cell lines indicated that the Trk tyrosine kinase inhibitor GNF-5837 reduced cell viability, through decreased phospho-TrkA (Tyr490) and downstream AKT (Ser473) activation, but did not display synergy with Herceptin. Overall, these data highlight a relationship between the tyrosine kinase receptors TrkA and HER2 and suggest the potential of TrkA as a novel or adjunct target for HER2-positive breast tumours.
Evaluation of Automatic Segmentation of Thalamic Nuclei through Clinical Effects Using Directional Deep Brain Stimulation Leads: A Technical Note
Authors: Krüger Marie T. , Kurtev-Rittstieg Rebecca , Kägi Georg , Naseri Yashar , Hägele-Link Stefan , Brugger Florian
Published: September 2020

Automatic anatomical segmentation of patients’ anatomical structures and modeling of the volume of tissue activated (VTA) can potentially facilitate trajectory planning and post-operative programming in deep brain stimulation (DBS). We demonstrate an approach to evaluate the accuracy of such software for the ventral intermediate nucleus (VIM) using directional leads. In an essential tremor patient with asymmetrical brain anatomy, lead placement was adjusted according to the suggested segmentation made by the software (Brainlab). Postoperatively, we used directionality to assess lead placement using side effect testing (internal capsule and sensory thalamus). Clinical effects were then compared to the patient-specific visualization and VTA simulation in the GUIDE™ XT software (Boston Scientific). The patient’s asymmetrical anatomy was correctly recognized by the software and matched the clinical results. VTA models matched best for dysarthria (6 out of 6 cases) and sensory hand side effects (5/6), but least for facial side effects (1/6). Best concordance was observed for the modeled current anterior and back spread of the VTA, worst for the current side spread. Automatic anatomical segmentation and VTA models can be valuable tools for DBS planning and programming. Directional DBS leads allow detailed postoperative assessment of the concordance of such image-based simulation and visualization with clinical effects.
Identification of the Criteria for Building Maintenance Decisions in Facility Management: First Step to Developing a Multi-Criteria Decision-Making Approach
Authors: Besiktepe Deniz , Ozbek Mehmet , Atadero Rebecca
Published: September 2020

Building maintenance is a fundamental practice in facility management, which supports the longevity of a building. Increasing costs of maintenance practices is a challenge for facility management professionals. Given that, building maintenance decisions often comprise complex and conflicting criteria. The primary purpose of this study is to develop and rank a set of criteria needed for constructing a multi-criteria decision-making model for use in building maintenance processes. This study also has an exploratory aspect and tries to establish the decision-making and condition assessment practices currently used in facility management. To do so, a literature review was conducted to reveal the significant criteria for building maintenance decision-making processes. Moreover, the results of a nationwide survey conducted with the members of two globally recognized facility management organizations were utilized. Identified criteria address a gap in facilities management research, i.e., the lack of comprehensive criteria in building maintenance decision-making, and can be used for the development of a multi-criteria decision-making model for use in building maintenance processes. Furthermore, the results of this study can help establish the current status of decision-making and condition assessment practices in facility management.
Erratum: Lee, H.H., et al. Histone 2A Family Member J Drives Mesenchymal Transition and Temozolomide Resistance in Glioblastoma Multiforme. Cancers 2020, 12, 98
Authors: Lee Hsun-Hua , Lin Che-Hsuan , Lin Hui-Yu , Kuei Chia-Hao , Zheng Jing-Quan , Wang Yuan-Hung , Lu Long-Sheng , Lee Fei-Peng , Hu Chaur-Jong , Wu Dean , Lin Yuan-Feng
Published: September 2020

The authors wish to make the following changes to this paper [...]
Essential Oils and Their Main Chemical Components: The Past 20 Years of Preclinical Studies in Melanoma
Authors: Di Martile Marta , Garzoli Stefania , Ragno Rino , Del Bufalo Donatella
Published: September 2020

The last two decades have seen the development of effective therapies, which have saved the lives of a large number of melanoma patients. However, therapeutic options are still limited for patients without BRAF mutations or in relapse from current treatments, and severe side effects often occur during therapy. Thus, additional insights to improve treatment efficacy with the aim to decrease the likelihood of chemoresistance, as well as reducing side effects of current therapies, are required. Natural products offer great opportunities for the discovery of antineoplastic drugs, and still represent a useful source of novel molecules. Among them, essential oils, representing the volatile fraction of aromatic plants, are always being actively investigated by several research groups and show promising biological activities for their use as complementary or alternative medicine for several diseases, including cancer. In this review, we focused on studies reporting the mechanism through which essential oils exert antitumor action in preclinical wild type or mutant BRAF melanoma models. We also discussed the latest use of essential oils in improving cancer patients’ quality of life. As evidenced by the many studies listed in this review, through their effect on apoptosis and tumor progression-associated properties, essential oils can therefore be considered as potential natural pharmaceutical resources for cancer management.
Cancer-Associated Fibroblasts: Versatile Players in the Tumor Microenvironment
Authors: Ganguly Debolina , Chandra Raghav , Karalis John , Teke Martha , Aguilera Todd , Maddipati Ravikanth , Wachsmann Megan B. , Ghersi Dario , Siravegna Giulia , Zeh Herbert J. , Brekken Rolf , Ting David T. , Ligorio Matteo
Published: September 2020

Cancer-associated fibroblasts (CAFs) are indispensable architects of the tumor microenvironment. They perform the essential functions of extracellular matrix deposition, stromal remodeling, tumor vasculature modulation, modification of tumor metabolism, and participation in crosstalk between cancer and immune cells. In this review, we discuss our current understanding of the principal differences between normal fibroblasts and CAFs, the origin of CAFs, their functions, and ultimately, highlight the intimate connection of CAFs to virtually all of the hallmarks of cancer. We address the remarkable degree of functional diversity and phenotypic plasticity displayed by CAFs and strive to stratify CAF biology among different tumor types into practical functional groups. Finally, we summarize the status of recent and ongoing trials of CAF-directed therapies and contend that the paucity of trials resulting in Food and Drug Administration (FDA) approvals thus far is a consequence of the failure to identify targets exclusive of pro-tumorigenic CAF phenotypes that are mechanistically linked to specific CAF functions. We believe that the development of a unified CAF nomenclature, the standardization of functional assays to assess the loss-of-function of CAF properties, and the establishment of rigorous definitions of CAF subpopulations and their mechanistic functions in cancer progression will be crucial to fully realize the promise of CAF-targeted therapies.
Visible Light Responsive Strontium Carbonate Catalyst Derived from Solvothermal Synthesis
Authors: Wichannananon Pornnaphat , Kobkeatthawin Thawanrat , Smith Siwaporn Meejoo
Published: September 2020

A single crystalline phase of strontium carbonate (SrCO3) was successfully obtained from solvothermal treatments of hydrated strontium hydroxide in ethanol (EtOH) at 100 °C for 2 h, using specific Sr:EtOH mole ratios of 1:18 or 1:23. Other solvothermal treatment times (0.5, 1.0 and 3 h), temperatures (80 and 150 °C) and different Sr:EtOH mole ratios (1:13 and 1:27) led to formation of mixed phases of Sr-containing products, SrCO3 and Sr(OH)2 xH2O. The obtained products (denoted as 1:18 SrCO3 and 1:23 SrCO3), containing a single phase of SrCO3, were further characterized in comparison with commercial SrCO3, and each SrCO3 material was employed as a photocatalyst for the degradation of methylene blue (MB) in water under visible light irradiation. Only the 1:23 SrCO3 sample is visible light responsive (Eg = 2.62 eV), possibly due to the presence of ethanol in the structure, as detected by thermogravimetric analysis. On the other hand, the band gap of 1:18 SrCO3 and commercial SrCO3 are 4.63 and 3.25 eV, respectively, and both samples are UV responsive. The highest decolourisation efficiency of MB solutions was achieved using the 1:23 SrCO3 catalyst, likely due to its narrow bandgap. The variation in colour removal results in the dark and under visible light irradiation, with radical scavenging tests, suggests that the high decolourisation efficiency was mainly due to a generated hydroxyl-radical-related reaction pathway. Possible degradation products from MB oxidation under visible light illumination in the presence of SrCO3 are aromatic sulfonic acids, dimethylamine and phenol, as implied by MS direct injection measurements. Key findings from this work could give more insight into alternative synthesis routes to tailor the bandgap of SrCO3 materials and possible further development of cocatalysts and composites for environmental applications.
Morphology-Governed Performance of Plasmonic Photocatalysts
Authors: Wei Zhishun , Janczarek Marcin , Wang Kunlei , Zheng Shuaizhi , Kowalska Ewa
Published: September 2020

Plasmonic photocatalysts have been extensively studied for the past decade as a possible solution to energy crisis and environmental problems. Although various reports on plasmonic photocatalysts have been published, including synthesis methods, applications, and mechanism clarifications, the quantum yields of photochemical reactions are usually too low for commercialization. Accordingly, it has been proposed that preparation of plasmonic photocatalysts with efficient light harvesting and inhibition of charge carriers’ recombination might result in improvement of photocatalytic activity. Among various strategies, nano-architecture of plasmonic photocatalysts seems to be one of the best strategies, including the design of properties for both semiconductor and noble-metal-deposits, as well as the interactions between them. For example, faceted nanoparticles, nanotubes, aerogels, and super-nano structures of semiconductors have shown the improvement of photocatalytic activity and stability. Moreover, the selective deposition of noble metals on some parts of semiconductor nanostructures (e.g., specific facets, basal or lateral surfaces) results in an activity increase. Additionally, mono-, bi-, and ternary-metal-modifications have been proposed as the other ways of performance improvement. However, in some cases, the interactions between different noble metals might cause unwanted charge carriers’ recombination. Accordingly, this review discusses the recent strategies on the improvements of the photocatalytic performance of plasmonic photocatalysts.