bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of two polishing systems on color and surface roughness of feldspathic porcelain following orthodontic bracket debonding and composite resin removal
Authors: Ameli Nazila , Talaei Reihane , Saeedi Saeedeh , Ghorbani Raheb , Askari Bahareh Ali
Published: July 2019

Objectives

Orthodontic bracket removal from a porcelain crown can roughen the surface and lead to plaque accumulation, discoloration, and esthetic problems. Porcelain polishing after debonding is one strategy to decrease such consequences. This study aimed to compare the efficacy of two polishing systems (Sof-Lex discs and Meisinger polishing system) for correction of surface roughness and discoloration of porcelain after orthodontic bracket debonding.

Materials and Methods

Twenty porcelain blocks were evaluated in two groups of 10. First, the baseline surface roughness and color parameters of the samples were measured using atomic force microscopy and spectrophotometry, respectively. After bracket bonding, a fine cutter was used for bracket debonding, and resin remnants were removed by a tungsten carbide bur and low-speed handpiece. Samples were then polished using Sof-Lex discs (group 1) and Meisinger porcelain polishing kit (group 2). Surface roughness and color parameters were measured again. Data were analyzed using SPSS 18 through the Shapiro–Wilk test, Student’s t-test, and paired t-test at 5% level of significance.

Results

Porcelain color change (ΔE) was significantly greater in the Meisinger system than Sof-Lex (P < 0.001). The Rq, Ra, and Rt surface roughness parameters significantly increased in both the groups after the intervention compared to baseline (P < 0.05), but the two groups were not significantly different in this respect after the polishing procedures (P > 0.05).

Conclusion

The porcelain color after polishing with Sof-Lex discs was closer to the baseline. Furthermore, the two systems were not significantly different regarding surface roughness. However, Sof-Lex discs may be recommended due to lower cost.
Orthodontic treatment in National Dental Centre of Singapore: Trends toward higher proportion of adult patients
Authors: Yen Tan Elaine Li , Song Yi Lin , Zhang Zhengyi , Jun Yong Kevin Jia , Zhang Yang , Yeo Xuening , Sim Yu Fan
Published: July 2019

Background

Adult patients used to be daunted with the prospect of having fixed appliances to correct malocclusions. However, this has gradually changed and recent literature reported an increasing trend in adults receiving orthodontic treatment. To date, there has been no information regarding the trend of adult orthodontic patients in Singapore.

Objective

The objective of this study was to determine the changes in the proportion of adult patients seeking orthodontic treatment at the National Dental Centre Singapore (NDCS) from 2011 to 2017.

Materials and Methods

The study sample consists of all patients who commenced active orthodontic treatment from 2011 to 2017 at the NDCS. The data collected were analyzed for the number, gender, and proportion of adult orthodontic patients treated annually from 2011 to 2017. Linear-by-linear association test was used to test for any significant linear trend in proportion of adult orthodontic patients over the 7 years. Pearson’s Chi-squared test was used to test for any significant gender differences.

Results

There was a significant increasing linear trend (p = 0.001) in the proportion of adult orthodontic patients from 2011 to 2017. No significant difference (p = 0.770) between the proportions of male and female adults undergoing orthodontic treatment was found. The ages of adults undergoing orthodontic treatment ranged from 21 to 73 years old and majority (48.61%) of them was in the 21–25 years old age range.

Conclusion

There is a rising trend in adults seeking orthodontic treatment at the NDCS from 2011 to 2017. No statistically significant gender difference was observed in this rising trend.
The prevalence of orthodontic treatment need and malocclusion problems in 8–9-year-old schoolchildren: A study in the south of Thailand
Authors: Rapeepattana Sirate , Thearmontree Angkana , Suntornlohanakul Supanee
Published: July 2019

Aims

This study aims to find the prevalence of orthodontic treatment need and malocclusion problems in 8–9-year-old schoolchildren in the south of Thailand.

Materials and Methods

A number of 202 children (100 boys and 102 girls) samples were randomly selected from all schools in Hat Yai District, Songkhla Province, Thailand. A cross-sectional survey of dental health component (DHC) of the Index of Orthodontic Treatment Need (IOTN) and malocclusion problems was investigated by clinical examination and dental model.

Results

Levels 4 and 5 of orthodontic treatment need according to DHC of IOTN of the sample were presented in 18.8% and 1.49%, respectively. Children who need orthodontic treatment (Grade 2–4) showed more than one highest DHC problem that indicated the level of treatment need (39.68%). Normal occlusion was found at 6.43%. Malocclusions such as Class I, Class II division 1, Class II division 2, and Class III malocclusion were observed in 78.71%, 7.92%, 3.47%, and 3.47%, respectively. Reversed overjet and overjet >9 mm were detected in 5.64% and 1.58%, respectively. Approximately half of the children (46.67%) had overbite >3.5 mm.

Conclusions

High percentage of children in mixed dentition period who need orthodontic treatment was found in this study. Some children who presented with the orthodontic treatment need Grade 2–4 had more than one DHC problem which identified the grade of treatment need. Class I malocclusion was most frequently found in this group of children.
Early treatment of dentoskeletal Class III malocclusion: SEC III protocol
Authors: Perillo Letizia
Published: July 2019

Dentoskeletal Class III malocclusions are one of the greatest challenges to the orthodontists due to the interaction of both environmental and genetic etiological factors. Over the years, several interceptive treatments for Class III dentoskeletal malocclusions have been proposed in growing patients. Our orthopedic approach includes two occlusal Splints combined with Class III Elastics and Chincup and so it is named SEC III protocol. The main difference with all the other appliances is the vertical control that is crucial, above all, in the most difficult cases as the hyperdivergent Class III malocclusions.
Early class III treatment decision-making
Authors: Ngan Peter , Musich David
Published: July 2019

Clinicians make decisions for their patients everyday. Ryan Hamilton, in his course guidebook, How You Decide: The Science of Human Decision Making, summarized the current research on the 4 R's of decision-making that matter: reference points, reasons, resources, and replacement. The authors will apply this principle in the decision- making necessary for the growing Class III patients. First, the decision on whether to treat or not to treat Class III patients in the mixed dentition rely on a thorough diagnosis and objectives for early treatment. For example, elimination of a functional shift of the mandible may be a good reason to institute early treatment. Second, the decision on when to start Phase II treatment relies on the follow-up observation after Phase I treatment. The authors suggested the use of a “checklist” to decide whether patient will be benefited from surgical intervention or nonsurgical orthodontic treatment. If the checklist review has several negative checkpoints, it will help the clinicians to decide on an aggressive stage of 4–8 months therapeutic re-diagnosis to confirm the surgical or nonsurgical decision.
Bone regeneration after alveolar dehiscence due to orthodontic tooth movement – A case report
Authors: Balut Nasib , Hansa Ismaeel , González Enrique , Ferguson Donald J.
Published: July 2019

This article presents the orthodontic treatment of a 15 year old male patient with an Angle class I malocclusion with a class II skeletal base, lower incisor proclination along with a hyperdivergent facial pattern. Such situations that involve camouflage treatment, usually results in further lower incisor proclination which can be reduced to an extent by adding buccal root torque. Placement of additional torque in this case however, resulted in positioning of the root apex of the lower right lateral incisor outside the alveolar housing, although no gingival signs were present. The mechanics were then reversed and at the end of 21 months of treatment, the apices were back within the alveolar housing. A 4-year post-treatment cone-beam computed tomography showed normal bone coverage of the affected tooth; and no clinical signs of gingival pathology were present. Orthodontists should be aware of possible complications of excessively torqueing lower incisors in order to prevent proclination. If root apices are inadvertently moved through the cortex, a good long-term prognosis is possible using orthodontics alone by reversing the mechanics, if no gingival complications are present.
Surgery-first orthognathic approach: A “scoping review” for mapping outcomes and plausible recommendations to develop core outcome sets
Authors: Gandedkar Narayan H. , Dávila María Mélita Chacón , Chng Chai Kiat , Liou Eric J. W. , Darendeliler Ali
Published: July 2019

Aims and Objectives

The aim of this scoping review was to identify the type of outcomes measured in surgery- first orthognathic approach (SFOA). The objectives were to classify the outcomes into predetermined domains and explore the degree of representation of each domain. Furthermore, to identify which domains are over- or under-represented and determine whether the findings of this scoping review could be employed to provide a template for core outcome sets (COS). Five outcomes were identified, and all the research pertinent to SFOA were assigned to these outcomes.

Materials and Methods

Electronic databases and additional records were searched from January 2009 to March 2019 to source the data, and 525 records were identified.

Results

The initial database and additional search resulted in 525 records, of which 54 potentially relevant articles were retrieved in full. 35 studies met the selection criteria following screening and were included in the scoping review with the results of the search depicted in the preferred reporting items for systematic reviews and meta-analyses. Domains such as morphological features or changes in maxillofacial skeleton and occlusion (n = 25, 71.42%) and psychosocial well-being including quality of life outcome (n = 8, 22.85%) were well represented while functional status (n = 1, 2.85%), health resource utilization (n = 0), and adverse effects (n = 1, 2.85 %) were under-represented.

Conclusions

Limited research on SFOA precludes development of COS. However, future SFOA clinical trials should consider underrepresented outcome domains to address the SFOA treatment modality comprehensively.
STATE OF RESOURCES AND FISHERY OF MANILA CLAM RUDITAPES PHILIPPINARUM (ADAMS ET REEVE, 1848) IN THE LOSOS BIGHT AND BUSSE LAGOON (ANIVA BAY, OKHOTSK SEA) IN 2010–2016
Authors: Kim A. Ch.
Published: July 2019

Landings of manila clam Ruditapes philippinarum were steady high in 2010–2016 and exceeded the recommended volume that caused deterioration of its resources in the areas of large aggregations. Any significant growth of the clam colonies in the surveyed area could not be expected in the nearest future because of continuing press of fishery. Total stock of Ruditapes philippinarum in the Aniva Bay is estimated as 240 t, including 203 t of clams with commercial size.
THE POPULATION PARAMETERS FOR RED KING CRAB IN THE DALNEZELENETSKAYA BAY (BARENTS SEA) IN THE SUMMER PERIOD OF 2013
Authors: Dvoretsky A. G. , Dvoretsky V. G.
Published: July 2019

Some biological parameters of the local group of rеd king сrаb in the Dalnezelenetskaya Bay (Barents Sea) are determined in summer 2013. The large-sized mature сrаbs with the carapace width > 100 mm prevailed in scuba diving catches, with equal proportion of females and males. The females with violet eggs dominated among the egg-bearing females. Barren females were not caught. Percentage of the limbs injuries was 62 % that was lower than in the previous year. Total stock of red king crab in the bay was assessed as 4750 individuals. Significant increasing of the large-sized males abundance is noted that corresponds with general growth of their stock in the Barents Sea after the ban on the species fishery within the 12-mile coastal zone in 2011.
CURRENT STATE OF STOCK FOR TANNER CRAB CHIONOECETES BAIRDI RATHBUN, 1924 (CRUSTACEA, DECAPODA) AT THE SOUTH-EASTERN COAST OF KAMCHATKA
Authors: Mikhailova O. G.
Published: July 2019

Tanner crab Chionoecetes bairdi is the highly valuable object of commercial fishery dwelling in the seas surrounding Kamchatka Peninsula. Its landings started at the southeastern coast of Kamchatka in early 1980s and continued till 2009, when the fishery was stopped by reasons independent on the stock state. It was launched again in 2017. Now the species is landed mostly in the Petropavlovskaya fishery subzone (62 % of the total allowable catch of tanner crab in Russia in 2019), whereas its stocks at southwestern Kamchatka and in the northwestern Bering Sea are rather low. Modern condition of the tanner crab population and its distribution at southeastern Kamchatka are considered on the data of the trap surveys conducted in spring and summer of 2012 and 2018. Between these years, commercial males of these species spread wider at southeastern Kamchatka and formed commercial aggregations in the northern Avachinsky Bay and in the southern and central parts of the Kronotsky Bay, but were rare southward from Cape Povorotny. They became larger: while size of the males varied in the range 50–170 mm in both years, the males with the carapace width < 120 mm prevailed in 2012, but the large-sized males with commercial size were more numerous in 2018. Percentage of the females in the catches was low both in 2012 and 2018 that is typical for the trap catches. The crabs at the 3rd stage of molting dominated both in May and August of 2012 and 2018. Injury level of the commercial males was rather high and increased from 2012 to 2018 in 8 %. Good current condition of the tanner crab population at southeastern Kamchatka is concluded.
USING OF POWERED PARAGLIDER IN THE TAUISKAYA GUBA BAY, THE OKHOTSK SEA, FOR LOCAL AIREAL SURVEYS AND OBSERVATIONS ON EARLESS SEALS
Authors: Gorshunov M. B.
Published: July 2019

Powered paraglider using for local aerial surveys and observations on real seals is described in combination with routine watch from the coastal observation point and motor boat. The results of conventional and combined observations are compared.
ON NEW FINDING OF MIRROR DORY ZENOPSIS NEBULOSA IN PETER THE GREAT BAY (JAPAN SEA)
Authors: Kim L. N. , Milovankin P. G.
Published: July 2019

New finding of rare fish species mirror dory Zenopsis nebulosa in Peter the Great Bay (Japan Sea) is described.
CHEMICAL COMPOSITION AND ENERGY CONTENT OF PACIFIC SALMONS IN THE OKHOTSK SEA
Authors: Gorbatenko K. M. , Melnikov I. V. , Ovsyannikov E. E. , Ovsyannikova S. L.
Published: July 2019

The main bioenergetic parameters of pacific salmons are presented on the data obtained in marine expeditions in 2003–2015. Among the examined species, the minimum energy content is registered in the muscle tissue of chum salmon and pink salmon, and the maximum energy content — in the muscles of king salmon. Total amount of the energy accumulated by juvenile pink salmon during its life within the Okhotsk Sea is estimated as 532 kcal or 27 % of the total energy accumulated in the whole marine period of its life, for juvenile chum salmon this amount is 492 kcal (11 %). The rest is accumulated by adults during their feeding in the ocean: pink salmon, without ranking by age and sex, gains in the North Pacific on average 1 442 kcal or 73 % of the total energy accumulated in the marine period of life, chum salmon — 4 071 kcal (89 %).
PRINCIPAL CLIMATE INDICES FOR THE NORTH PACIFIC: NATURE AND HISTORY (A REVIEW)
Authors: Khen G. V. , Ustinova E. I. , Sorokin Yu. D.
Published: July 2019

Climatic indices reflecting the environmental conditions and patterns of their variability in the entire northern Pacific and in its local regions are overviewed. Their physical nature and mechanisms of the processes, their geography and methods of calculation are presented, with citing of the first descriptions. Among a variety of global and regional climatic indices concern ing the North Pacific, the following ones are described: the indices of Arctic Oscillation (AO), El Niño — Southern Oscillation (ENSO), Pacific Decadal Oscillation (PDO), Aleutian Low Pressure Index (ALPI), Siberian High Index (SHI), North Pacific Index (NPI), Pacific/North American (PNA) Index, and West Pacific Index (WP). AO is a large-scale index of atmospheric circulation reflecting the processes both in the troposphere and stratosphere, where «pumping» of air masses between the high and moderate latitudes occurs continuously. ENSO is also a large-scale index that reflects large-scale interactions in the fields of temperature, atmospheric pressure, wind, and cloudiness over the whole Pacific. Other indices are rather regional, since their influence does not extend far beyond the limits of the domains of their definition. Nevertheless, their role in environmental fluctuations in certain areas could be significant and their influence could be traced throughout the Northern Hemisphere.
COMBINED EFFECT OF CADMIUM AND LOWERED SALINITY ON DYNAMICS OF ABUNDANCE AND PHYSIOLOGICAL PROCESSES FOR ALGA PLAGIOSELMIS PROLONGA
Authors: Markina Zh. V. , Aizdaicher N. A.
Published: July 2019

Combined effect of cadmium (10, 30, and 50 mg/L) and salinity (32, 24, and 16 ‰) on marine unicellular alga Plagioselmis prolonga (Cryptophyta) is investigated. Such parameters as the cells number dynamics, their size, side scattering (as a characteristic of internal structure of cells), photosynthetic pigments fluorescence (chlorophyll a, phycoerythrin, allophycocyanin, as a measure of the photosynthetic apparatus function), and reactive oxygen species (ROS) content (as an index of the cellular membranes destruction) were registered in experiments using the flow cytometer CytoFLEX (Beckman Coulter). Addition of cadmium in concentration of 10 mg/L to the water with salinity 32 ‰ did not influence on alga growth and its physiological parameters. The higher concentrations of cadmium, as 30 and 50 mg/L, stimulated the cells growth and ROS increased under the concentration of 50 mg/L, but other parameters did not altered. The same contamination with cadmium under salinity 24 ‰ caused the cells size rising and their intensive growth, ROS increasing on 3rd day and decreasing on 7th day of exposition, and phycoerythrin and allophycocyanin fluorescence diminishing, but the side scattering and chlorophyll a fluorescence still did not differ from the control. Any concentration of cadmium in the water with salinity 16 ‰ caused the population growth inhibition, decreasing of side scattering and all photosynthetic pigments fluorescence, and increasing of ROS and cells size. There is concluded that cadmium contamination in conditions of normal (32 ‰) salinity has a stimulation effect on Plagioselmis prolonga growth and no effect on its photosynthetic apparatus, but the same contamination in conditions of lowered salinity has a negative effect on both its growth and photosynthetic functions, whereas the reactive oxygen species content increases and the cells become larger under the cadmium contamination regardless the salinity factor.
NEMATODES OF GENUS PHILONEMA (PHILONEMIDAE) FROM SOCKEYE SALMON AND WHITESPOTTED CHAR FROM LAKE KISI (OLA RIVER BASIN, OKHOTSK SEA)
Authors: Pospekhov V. V. , Kusenko K. V.
Published: July 2019

Morphology of phylonems infesting whitespotted char Salvelinus leucomaenis and sockeye salmon Oncorhynchus nerka in Lake Kisi at the northern coast of the Okhotsk Sea is described for the first time. Sockeye was infected with adults of Philonema oncorhynchi Kuitunen-Ekbaum, 1933 and larvae of other nematode species (Philonema sp. II), and char — with adult dracunculoid nematode designated as Philonema sp. I. This Philonema sp. I differs considerably from Ph. oncorhynchi by number of reproductive papillae, their distribution, and structure of cuticular appendices on the caudal end of males. Larvae of Philonema sp. I (1st stage) and Philonema sp. II (3rd stage) are described, as well. These new data allow to reconsider the species belonging of nematodes infesting chars (Salvelinus) in North-East of Russia, which are identified now mostly as Ph. oncorhynchi.
FIRST EXPERIENCE OF CAGE CULTIVATION OF HATCHERY-PRODUCED JUVENILE SCALLOP MIZUHOPECTEN YESSOENSIS AT EASTERN COAST OF PRIMORYE
Authors: Gavrilova S. G. , Sukhin I. Yu. , Turabzhanova I. S.
Published: July 2019

Yesso scallop Mizuhopecten yessoensis is the main object of aquaculture in Primorye with annual yield measured in hundreds ton. However, further development of its farming could be restricted by lack of the spat. Availability of the spat imported from China using for the scallop cultivation at the coast of Primorye is explored. The spat from Chinese hatchery located in Shandong province was supplied for one aquaculture company in Primorye in the volume of 660 . 106 ind. in 2018 and settled to hanging collectors (1000 ind. per bag), then to cages in the marine farm located north-eastward from the Moryak-Rybolov Bay. Survival and growth rate of the scallop juveniles were examined in early November 2018, when the water temperature was from 10.3–10.4 оС at the sea surface to 9.7–9.9 оС at the bottom, salinity was about 33.60 psu in the whole water column, chlorophyll a concentration varied in the range 0.4–0.8 mg/L with the maximum in the layer 8–24 m. The spat mortality during transportation was estimated as 15 %, but it increased to 56–90 % during its growing up in cages. This level of mortality is much higher than mortality of the spat collected from the coastal waters at southern Primorye. The highest mortality of artificial spat was observed for large-sized mollusks, whereas it was satisfactory for the small-sized ones. Possible reason of high mortality is too high density of juveniles and damaging of their soft tissues by other shells. The spat shells height did not exceed 5–10 mm in the collectors but increased during their growing in cages to 20–25 mm. This size is similar to the size of scallop growing from natural spat in southern Primorye. However, growth rate of the scallop growing at the Moryak-Rybolov Bay (2.0–2.7 mm/month) is lower than in Peter the Great Bay (3.0–3.5 mm/month) though higher than in the Vladimir Bay and Aniva Bay. Mean body weight of the cultivated scallop in November was 1.5 ± 0.1 g.
FEATURES OF SEABIRD INTERACTION WITH TRAWL FISHING GEAR
Authors: Artukhin Yu. B.
Published: July 2019

Seabirds interaction with trawl fishing gear was observed aboard the large freezing-processing trawler «Moskovskaya Olimpiada» during the pollock fishery in the Okhotsk Sea in January-April, 2015. In total, 579 observation rounds were realized including 105 ones during the trawl setting, 116 ones during hauling, and 358 ones during trawling, with total duration of 280.7 hours. In the observations, 1443 contacts of birds with the fishing gear were recorded. Light contact of a flying gull with the trawl net was observed in one case only; all other contacts were collisions with the wires. The warps and the depth sounder cable were the greatest hazard for birds. Collisions with the sounder cable during the trawling were the most frequent, with approximately 5 times higher frequency than with the same cable during the trawl setting or hauling. Almost all strikes with the wires were registered for northern fulmars (97.5 %) and the rest of the strikes — for large gulls. Gulls had only light contacts with the depth sounder cable (2 contacts afloat and 34 contacts in flight). Fulmars had contacts both with the depth sounder cable and warps. Flying birds of this species contacted with the depth sounder cable more frequently (75.5 %) than those sitting on the water. On the contrary, majority of fulmars (61.6 %) contacted with the warps being afloat in the process of their feeding. Several cases only (0.6 %) of direct interactions with the fishing gear caused the bird’s death. All these cases occurred with the fulmars drowned when they fell under the depth sounder cable (7 ind.) or under the warp (1 ind.). Frequency of the bird collisions with the wires differed prominently by the fishery districts, with the maximum in the Kamchatka-Kuril subzone (8.35 contacts/hour for fulmars and 0.22 contacts/hour for gulls). These spatial differences are related to the birds abundance near the ship that depends on distribution patterns of certain bird species in the Okhotsk Sea in winter. General frequency of the contacts depends credibly on intensity of the waste discharges after fish processing and on wind direction relative to the ship course.
USING THE METHOD OF BUILDING OF OPTIMAL HAMILTONIAN CYCLE FOR INVESTIGATION OF SEASONAL CHANGES IN MACROPHYTOBENTOS (ON EXAMPLE OF THE VOSTOK BAY, JAPAN SEA)
Authors: Semkin B. I. , Varchenko L. I.
Published: July 2019

Method of building of optimal Hamiltonian cycle is proposed for study of cyclic changes in species composition of macrophytobenthos in the Vostok Bay. Compiled lists of macroalgae and higher plants are composed for each month. Requirements for the species lists comparing are established. The optimal Hamiltonian cycles for the lists without permanent species are different from those for the complete lists because of higher values of the measures of difference, particularly in winter. The Sørensen–Dice coefficient, Jaccard similarity index, or equivalent measures are recommended to use for assessment of the differences between the species lists.
COMPLEX MONITORING OF SALMON SPAWNERS ESCAPEMENT TO THE KAMCHATKA RIVER IN REAL TIME REGIME
Authors: Fadeev E. S. , Shevlyakov E. A. , Feldman M. G.
Published: July 2019

Complex method of quick quantitative evaluation of the spawners escapement to the Kamchatka River is developed for pacific salmons on the base of analysis of their passing dynamics through certain parts of the river (main stream, tributaries, spawning grounds). The runs of different temporal forms of anadromous salmons were separated taking into account their biological parameters using the data of biological analysis of commercial catches in Ust-Kamchatsky district. The spawners abundance was evaluated from CPUE value for the main stream where control catches of spawners were made in the periods predetermined for escapement, and directly using two duplicate hydroacoustic complexes for the tributary important for salmons reproduction or visually from ashore, boats, or flying vehicles for other tributaries. These data were coupled with general assessments of the escapement values in the river basin from helicopters. Numeral model was developed to evaluate the salmons escapement to the spawning grounds. The escapement dynamics was simulated retrospectively for sockeye, chum and coho salmon in 2016 and 2017. The method was successfully used for fishery management in the fishery campaign of 2018. Efficiency of the fishery regime was estimated using this approach. The value of sockeye salmon escapement was determined as enough for medium reproduction, and the values of chum and coho salmons escapement — as enough for medium or high reproduction. For chinook salmon, the model was not tested, but the method of control catches was developed and applied as a part of integrated methodology for quantitative operational assessment of escapement.
WHAT HAPPENED IN THE ECOSYSTEM OF THE OKHOTSK SEA IN 2008–2018?
Authors: Shuntov V. P. , Ivanov O. A. , Gorbatenko K. M.
Published: July 2019

Critical analysis is done of the main conclusions in the article published by Yury Zuenko and co-authors in this issue of «Izvestia TINRO» journal about changes in the Okhotsk Sea biota in 2008–2018. In that paper, a continuing warming in all layers of the sea is shown quite convincingly, but weight explanations of the observed changes in marine communities and commercial stocks are not offered. Moreover, the authors ignore results of long-term studies of the Okhotsk Sea bioresources conducted from ecosystem positions. From this alternative point of view, some integrated quantitative data on dynamics of zooplankton, zoobenthos, fish, and other biota in the Okhotsk Sea are presented, as well as the schemes of organic matter and energy flows through trophic networks that show a normal functioning of the Okhotsk Sea ecosystem at the present time. On the base of these data, doubts are claimed in correctness of Zuenko’s concept of the ecosystem restructuring in direction to lower productivity and higher efficiency of its functioning.
CLIMATE CHANGES AND CURRENT STATE OF BIOTA IN THE RUSSIAN WATERS OF THE FAR-EASTERN SEAS
Authors: Shuntov V. P. , Ivanov O. A.
Published: July 2019

Discussion about limiting influence of climate conditions on dynamics of the pacific salmon populations and generally on biological resources of the Far-Eastern Seas is continuing. Multifactor impacts of climate and oceanographic conditions on populations and communities are concluded that cannot be described only by climatic indices and anomalies of the surface water temperature in the ocean in winter. Dynamics of the fish and invertebrates populations in various regions of the Far-Eastern Seas show a positive effect of climate warming on state and sustainability of the resource base for fisheries.
A comparative study on the effectiveness of PNF stretching versus static stretching on Pain and Hamstring flexibility in osteoarthritis knee patients
Authors: Arul Pragassame S  , Mohandas Kurup VK  , Soundarya N
Published: July 2019

Osteoarthritis (OA) knee is a long-term chronic disease characterized by the destruction of articular cartilage and underlying bone. Pain, limitation of motion and functional impairment are common clinical features. Poor hamstring flexibility is a major problem for patients with OA knee. The stretching of the hamstring is a necessary intervention in the management of the OA knee. This study is therefore intended to compare the effectiveness of static stretching vs PNF stretching for pain, hamstring flexibility and functional mobility in OA Knee patients. 30 patients were randomly divided into two groups. Group A (N=15) received PNF stretching, wax therapy and isometric quadriceps exercises, and group B (N=15) received static stretching, wax therapy and isometric quadriceps exercises. The intensity of the pain was measured using the NPRS (Numerical Pain Rating Scale), hamstring flexibility by AKET (Active Knee Extension Test) and functional mobility by TUG (Timed up and Go) test. The results showed that significant differences in NPRS (Z=4.64, P=0.001), AKET (t=9.61, P=0.001) and TUG (t=8.19, P=0.001) were observed in group A patients when compared to group B. In conclusion, the PNF stretching treatment program is effective in reducing pain, improving hamstring flexibility and functional mobility in patients with OA knee compared to static stretching.
Molecular detection of Candida species by Restriction Fragment Length Polymorphism (RFLP) analysis of PCR from HIV infected persons
Authors: SandhyaRani T  , Srikumar R  , Prabhakar Reddy E  , Latha S
Published: July 2019

Oral Candidiasis an important opportunistic fungal infection in HIV patients. This is the first sign of HIV infection. It can spread from the mouth through the pharynx to the oesophagus. Oral Candidiasis is most regularly complicated with oesophagal Candidiasis, which may restrain nourishment and result in weight reduction, alarming the overall health of the HIV tainted persons. In present study, we have a tendency to determine in Candida species by blend of Restriction Fragment Length Polymorphism (RFLP) examination of PCR from HIV tainted people and furthermore we demonstrated that phenotypic and genotypic identification method was vital to work out the species. We recognized seventy two Candida confines acquired from a hundred and fifty patients contaminated with HIV by utilizing PCR-RFLP assay. All inclusive primers for the internal transcribed spacer (ITS) area (ITS1– ITS4) of the fungal DNA qualities were utilized for this measure. We distinguished the diverse Candida spp. By utilizing the limitation enzyme MspI. Here most of the isolates were obtained from male patients 40(51%). Candida albicans was the recurrent species segregated (46.8%) pursued by C. tropicalis (30.3%), C. glabrata (7.59%), C. parapsilosis (6.3%) C.dubulinesis (6.3%) along with C. krusei (2.5%) detected successfully. PCR- RFLP is a high definite, sensitive, quick, reliable technique and also applicable method for fungal detection in clinical laboratory for identification of medically important Candida spp. It is used for detection of mycological examinations in HIV-positive along with other immunocompromised patients for epidemiological studies.
Formulation and evaluation of bilayer tablets: Glimepiride in floating drug delivery and Metformin in sustained release
Authors: Harish Choodappa  , Subramanian Somaskandan
Published: July 2019

In this study, we aimed to prepare bilayer tablets of Glimepiride in floating drug delivery and Metformin in sustained release formulation. Glimepiride is chosen in floating drug delivery to overcome the gastric irritation and gastric emptying time. Glimepiride was prepared by different polymers such as guar gum, xanthan gum, carbopol and sodium bicarbonate act as effervescent agent, and other excipients were mixed and compressed by direct compression as the first layer.  Metformin was chosen in sustained release to reduce dose frequency using different polymer HPMC K100M, methylcellulose, PVP K30 in different ratio and other excipients were mixed and compressed as the second layer. The first layer and second layer are combined as a bilayer tablet. Tablets were evaluated for hardness, friability, thickness, weight variation; the In vitro studies were done for all formulation. Among all formulation, F2 was selected as best formulation and release kinetics studies were evaluated for formulation F2.
Enantiomeric separation of Midodrine hydrochloride in bulk and pharma-ceutical dosage form by chiral HPLC
Authors: Jenifer Ashwini S  , Narenderan ST  , Meyyanathan SN  , Babu B  , Gowramma B
Published: July 2019

A simple, sensitive chiral liquid chromatographic method was developed for the separation and quantification of enantiomers Midodrine. A chiral PAK IG-3 (150 x 4.6 mm) 3µm column was used for the separation of the enantiomers. The mobile phase consists of 10 mM ammonium bicarbonate in water and acetonitrile in the ratio of 95:5, v/v with a flow rate of 0.7 ml/min. The detection was done at 290 nm with column temperature maintained at 40°C. The method linear ranged between 10 – 110 μg/ml and 5 – 100 μg/ml for (+) and (-) Midodrine enantiomers.  The recovery of the method was found to be in the range of 99.1 to 101.2 %. The detection limit for the (+) and (-) enantiomers was found to be 4 μg/ml and 1 μg/ml, respectively. A simple validated chiral HPLC method with reverse elusion is described for the separation and quantification of the enantiomers of Midodrine in bulk and formulation.
Convolution Neural Network Based Ecg Classifier
Authors: Sharanya S  , Sridhar PA  , Poornakala J  , Muppala Vasishta  , Tharani U
Published: July 2019

Classification of Electrocardiogram (ECG) signals plays a significant role in the identification of the functioning of the heart. This work pertains with the ECG signals, where the classifier is developed for identification of normal or abnormal conditions of the heart. The raw ECG signals are collected from an online database (www.physioNet.org) for classification. The raw ECG signal is pre-processed for noise removal, and the frequency spectrum is analysed to compare raw and denoised ECG signal. Attributes (P, Q, R, S, T time intervals) from denoised ECG signal is analysed and classified using Convolution Neural Network (CNN). The paper reports a classification technique to differentiate ECG signals from the MIT-BIH database (arrhythmia database, arrhythmia p-wave annotations, atrial fibrillation). The CNN analyses the deviation between nominal ranges of attributes (amplitude and time interval) and classifies between the abnormality and normal ECG wave. This work provides a simple method for interpreting ECG related condition for the clinician and helps medical practitioners to make diagnostic decisions.
Simultaneous estimation of ledipasvir and sofosbuvir in bulk and its dosage forms by stability indicating RP-HPLC method
Authors: Ismail Y  , Vijaya Vara Prasad M
Published: July 2019

The fixed-dose ledipasvir-sofosbuvir combination offers an effective and well-tolerated pill for the treatment of chronic hepatitis C infection. Only a few analytical works were carried out to estimate the Ledipasvir and Sofosbuvir drug combination in the various dosage forms. This work aimed to simplify the estimation process using RP-HPLC methodology. The method was developed on a reversed phase Agilent C18 (4.6 x 150 mm, 5 µm) column. The isocratic elution process was performed using a mobile phase ratio of Methanol (70% v/v): Water (30 % v/v) with 0.6 ml/min flow rate. Elute was scanned using the PDA detector at the wavelength of 235 nm. The results of the elution process showed that the Ledipasvir and Sofosbuvir elute the peak at a concentration of 9 μg/ml and 40 μg/ml with retention times of 7.745 min and 2.345 min respectively. The percentage purity of Ledipasvir and Sofosbuvir was found to be 99.40 % w/v and 98.20 % w/v. The proposed method was found to be a high degree of precision and reproducibility. The percentage recovery was found to be 99.92 % for Ledipasvir and 99.82 % for Sofosbuvir. The LOD and LOQ were measured, and the results were within limits. The developed validation method can be applied for degradation evaluation of Ledipasvir and Sofosbuvir for the various dosage forms.
Assessment and prevalence of depression among cardiovascular disease patients in Nilgiris District observational cross-sectional study
Authors: Anand Vijayakumar PR  , Asem Veeves Singh  , Asish Kumar Saha  , Kavuri Srikar
Published: July 2019

Depression deter mental catabolic effect over the time, symptomatically affects quality of life which leads to worse health outcomes that linked to higher mortality and morbidity rates with huge impact on public health. Chronic conditions like Cardio Vascular Diseases (CVDs) deteriorates patient health with cognitive impairment that progresses to somatic dysfunction, which can exacerbate to the occurrence and development of depression with the increased risk of inducing Chronic Diseases. Depression are often neglected, overlooked and remains unrecognized and undertreated in the patient, especially in CVDs patient are considered as normal that instances to poor prognosis.  The association of depression and CVDs are not incidental, but the lack of diagnosis for the coexistence of these co-morbid condition and poor treatment hindrance the disease outcome. Depression emerges an alarming cardio vascular risk factor which is associated with higher cost of care for the patient as well as burden to the healthcare system. Prior detection and identification with rational intervention of depression at CVDs will help to change the scenario by improving the standard of health and overall quality of life of patient. Here, in this study we assess the prevalence of depression at cardio vascular disease patient in Nilgiris population.
Hepatoprotective effect of vitamin A and E on diclofenac induced hepatotoxicity in male Wistar albino rats
Authors: Siva T  , Girija Sivakumar  , Sankaran PK  , Yuvaraj Maria Francis  , Gayathri T  , Lakshmi Thangavelu  , Karunakarn Balaji
Published: July 2019

This study was done to show the changes in the liver following diclofenac treatment and to study the hepatoprotective effects of Vitamin E and A in diclofenac treated rats. Rats were divided into four groups of six rats each. Group-1: Control rats (n= 6), Group-2: Rats (n= 6) treated with diclofenac at dose of 50 mg/kg IM for 7 days, Group-3: Rats (n= 6) treated with Vitamin A at dose of 400 IU/kg orally followed by diclofenac at 50 mg/kg IM 2 h later for 7 days, and Group 4: Rats (n= 6) treated with Vitamin E at dose of 200 IU/kg orally followed by diclofenac at 50 mg/kg IM 2 h later for 7 days. Later it was analysed with standard biomarkers, and it was histologically interpreted. The results showed that there was an rapid increase in the levels of liver function test in diclofenac treated group, which was significantly decreased after pre-treatment with vitamin E than vitamin A. The liver acinus showed centriacinar necrosis of hepatocytes after 7 days of diclofenac treatment, which was prevented by administration of Vitamin E and A. Drug-induced liver injury possesses a major clinical problem and has become a leading cause of acute liver failure and transplantation. Overstressed liver compromises its detoxification role which may expose it to a variety of diseases and disorders. Diclofenac sodium is a phenylacetic acid derivative, a widely used nonsteroidal anti-inflammatory drug (NSAID) for the treatment of inflammatory conditions such as osteoarthritis, rheumatoid arthritis, polymyositis, dermatomyositis, dental pain and spondyloarthritis. Although the exact mechanism by which diclofenac injuries in liver is not understood, some studies explain the toxicity by affecting cytochrome P 450 leading to the production of active metabolites. Hepatoprotective effects of Vitamin E were better compared to Vitamin A following treatment with NSAIDS. Hence, it may be necessary to administer Vitamin E in patients treated with diclofenac.
Antioxidant effects of vitamin E on diclofenac induced hepatotoxicity in male rats
Authors: T. Siva  , Girija Sivakumar  , Sankaran PK  , Yuvaraj Maria Francis  , Gayathri T  , Kumaresan M  , Karunakarn Balaji
Published: July 2019

Drug induced liver injury possesses a major clinical problem and has become leading cause of acute liver failure and transplantation. Overstressed liver compromises its detoxification role which may expose it to a variety of diseases and disorders. The present study was to determine whether pre- administration of various doses of vitamin E would have protective effect against diclofenac induced hepatotoxicity in wistar male rats. Twenty-four albino male rats weighing 180-200g were divided equally into four groups. In control group rats were administrated with physiological saline 2ml/kg b.wt /intramuscularly. Another group with 50mg/kg b.wt/ intramuscularly/seven days diclofenac was used for inducing toxicity. In experimental groups rats were administrated with different doses of vitamin E along with diclofenac sodium [200 and 400 IU orally and 50mg/kg b.wt/ intramuscularly/seven days]. Showed that there was a rapid increase in the levels of liver function test in diclofenac treated group, which was significantly decreased after pre-treatment with high dose than low dose of vitamin E. The liver acinus showed Centro acinar necrosis of hepatocytes after 7 days of diclofenac treatment, which was prevented by administration of Vitamin E. Drug-induced liver injury possesses a major clinical problem and has become a leading cause of acute liver failure and transplantation. Overstressed liver compromises its detoxification role which may expose it to a variety of diseases and disorders. Diclofenac sodium is a phenylacetic acid derivative, a widely used nonsteroidal anti-inflammatory drug (NSAID) for the treatment of inflammatory conditions such as osteoarthritis, rheumatoid arthritis, polymyositis, dermatomyositis, dental pain, spondyloarthritis, acute migraine, gout attacks and pain management in gall and renal stones. Although the exact mechanism by with diclofenac injuries liver is not understood, some studies explain the toxicity by affecting cytochrome P 450 leading to the production of active metabolites. Administration of different dose of diclofenac sodium induces severe adverse effects in liver and kidney.
Anticancer potential of the extract of Melothoria maderaspatana on DMH induced colon cancer in albino rats
Authors: Ramachandran K  , Venketnarayanan R
Published: July 2019

Cancers of the large and small intestine are major contributors to worldwide cancer morbidity and mortality. Out of all the cancers, colon cancer is one of the most common diseases in the world. Every year 1.2 million patients are diagnosed for colon cancer. The rate of colon cancer incidence was low in India but is presently increasing; out of 3.5 million cancer cases, 35,000 have colon cancer. As a part of chemotherapy, lots of anticancer drugs are in the market, but the main problem associated with these drugs is their side effects. Because of chemotherapy treatment side effects, the patient needs secondary palliative care treatment. Plant medicines are well known for their non-toxic side effects, so the objective of the study is to develop a drug from medicinal plant against colon cancer with non-toxic side effects. It plays an important role in the discovery of lead compound for the development of conventional drugs. About 60% of currently used anticancer agents are derived from a natural source. In the present study, Melothoria maderaspatana was used to study the anticancer potential of the extract and to synthesize new anticancer moiety. With the findings of the study, it can be concluded that the plant Melothoria maderaspatana and possess anti-colorectal cancer activity. Before the clinical usage of extract, thorough toxicological profile has to be determined on the crude extracts as well as on isolated compounds to confirm the safety of the drug.
Antifungal susceptibility testing of candida – Methods and issues in current practice
Authors: Lakshmi Krishnasamy  , Priya Santharam  , Chitralekha Saikumar
Published: July 2019

Despite the availability of many antifungal drugs in clinical practice, the occurrence of antifungal drug resistance is on the rise. Since the antifungal susceptibility testing (AFST) is not done routinely in many of the microbiology laboratories, it is very difficult to determine which antifungal agent is very effective for a particular infection. There is a real need for precise, reproducible and extrapolative antifungal susceptibility testing methods to aid the therapeutic management. The practice of empirical treatment for fungal infections further promotes the emergence of resistant strains. The AFST practice would essentially help the clinicians in appropriate decision making. Although conventional AFST methods are somewhat cumbersome, many novel AFST methods are currently available in many laboratory settings which would provide a quicker result many times. In essence, the application of AFST along with identification of the fungus up to species level would definitely be very helpful in selecting the primary antifungal agents for treatment especially in difficult to manage and invasive fungal infections. This review will throw light on the various AFST methods available and their issues in the current practice.