bluuebunny/crossref_metadata_2025 huggingface dataset:
On the possible contribution of cationic oxygenated carbon chains CnO+, HCnO+, and OCnO+ (n = 4–9) to the diffuse interstellar bands
Authors: Jacovella U , Buntine Jack T , Cotter Mariah , Muller Giel , Scholz Michael S , Dartois Emmanuel
Published: January 2022

Only four of the diffuse interstellar bands (DIBs) are currently accounted for, ascribed to electronic transitions of C$_{60}^+$. Investigations into carriers of other DIBs historically focus on charged and neutral hydrocarbons, and little information is available regarding oxygenated carbon and hydrocarbon species that result from the two most abundant heavy elements in the interstellar medium, C and O. In this study, we assess whether CnO+, HCnO+, and OCnO+ (n = 4–9) cations are viable candidates to account for DIBs using both density-functional theory (DFT) and coupled cluster single-double and perturbative triple theory, CCSD(T). For these species, the linear structures are the most stable isomers with the lowest dissociation threshold corresponding to CO loss. Optical absorptions of the oxygenated carbon chain cations are characterized by calculated vertical excitation wavelengths and their corresponding oscillator strengths using the equation-of-motion CCSD (EOM-CCSD) method. Aside from HC4O+ and HC2n +1O+, all of the species considered in this study have calculated electronic transitions that lie in the visible or near-infrared spectral regions. Minimal column densities necessary for these cations to account for DIBs have been estimated. Based on present results and the known column densities for neutral oxygenated carbon chains in TMC-1, the growth rate of charged O-bearing carbon chains via ion-neutral reaction mechanisms is judged to be too low to form a sufficient population to give rise to DIBs.
Global comparison of awake and asleep mapping procedures in glioma surgery: An international multicenter survey
Authors: Gerritsen Jasper K W , Broekman Marike L D , De Vleeschouwer Steven , Schucht Philippe , Jungk Christine , Krieg Sandro M , Nahed Brian V , Berger Mitchel S , Vincent Arnaud J P E
Published: January 2022

Background

Mapping techniques are frequently used to preserve neurological function during glioma surgery. There is, however, no consensus regarding the use of many variables of these techniques. Currently, there are almost no objective data available about potential heterogeneity between surgeons and centers. The goal of this survey is therefore to globally identify, evaluate and analyze the local mapping procedures in glioma surgery.

Methods

The survey was distributed to members of the neurosurgical societies of the Netherlands (Nederlandse Vereniging voor Neurochirurgie—NVVN), Europe (European Association of Neurosurgical Societies—EANS), and the United States (Congress of Neurological Surgeons—CNS) between December 2020 and January 2021 with questions about awake mapping, asleep mapping, assessment of neurological morbidity, and decision making.

Results

Survey responses were obtained from 212 neurosurgeons from 42 countries. Overall, significant differences were observed for equipment and its settings that are used for both awake and asleep mapping, intraoperative assessment of eloquent areas, the use of surgical adjuncts and monitoring, anesthesia management, assessment of neurological morbidity, and perioperative decision making. Academic practices performed awake and asleep mapping procedures more often and employed a clinical neurophysiologist with telemetric monitoring more frequently. European neurosurgeons differed from US neurosurgeons regarding the modality for cortical/subcortical mapping and awake/asleep mapping, the use of surgical adjuncts, and anesthesia management during awake mapping.

Discussion

This survey demonstrates the heterogeneity among surgeons and centers with respect to their procedures for awake mapping, asleep mapping, assessing neurological morbidity, and decision making in glioma patients. These data invite further evaluations for key variables that can be optimized and may therefore benefit from consensus.
Diverse Anatexis in the Main Central Thrust Zone, Eastern Nepal: Implications for Melt Evolution and Exhumation Process of the Himalaya
Authors: Liu Shuaiqi , Zhang Guibin , Zhang Lifei , Wang Shuzhen , Upreti Bishal N , Adhikari Danda P , Wu Chenguang , Wang Jiaxing
Published: January 2022

Sitting between the Greater Himalayan sequence (GHS) and Lesser Himalayan sequence (LHS), the Main Central Thrust zone (MCTZ) has experienced multiple episodes of anatexis, which presents an opportunity to explore the nature of partial melting and its response to Himalayan orogenic processes. A series of deformed rocks, including migmatites, gneisses, and leucosomes were collected across the MCT at Arun Valley, eastern Nepal. We investigated the bulk rock major and trace elements, Sr-Nd isotopes, mineral chemistry, zircon geochronology and Hf isotopes, and conducted phase equilibria modeling. The protolith boundary between the GHS and LHS is recognized on the basis of Sr–Nd isotopes with εNd(0) of −16.7 to −8.0 for the GHS and −31.2 to −23.9 for the LHS. Samples from both the GHS and LHS have undergone partial melting, as revealed by in situ leucosomes at outcrops and melt inclusions at thin-section scale. Leucosomes separated from their host rocks are divided into four groups: those derived from hydration melting, muscovite dehydration melting, amphibole dehydration melting, and feldspar accumulation. Phase equilibria modeling results for the GHS migmatite show isothermal decompression from peak P–T conditions of 11 kbar and 795°C, accompanied by muscovite dehydration melting evolving into biotite dehydration melting. In contrast, rocks from the LHS are modeled to have undergone hydration melting at P–T conditions of 9 kbar and 685°C. Zircon U–Pb geochronology suggests that long-lived partial melting (35–13 Ma) occurred in the MCTZ. Moreover, anatectic zircon Hf isotopes show that the protoliths for partial melting changed from the GHS to the LHS with εHf(t) of −19.4 to −5.7 during the early Miocene, and lower values of −42.5 to −16.7 during the middle to late Miocene. These zircon geochemical results indicate that hydrous metasediments from the LHS were progressively accreted to the base of the GHS, resulting in hydration melting of both the GHS and LHS assisted by MCT. The timing of activity of the MCT is constrained to 25–13 Ma, coeval with movement of the South Tibetan detachment system. Integration of petrogenetic modeling, the chronology of partial melting, and metamorphic P–T paths allows us to propose that thickened Himalayan crust was heated from the middle to late Eocene, and widespread anatexis occurred during the Oligocene to middle Miocene, forming a large-scale melt channel. The hot GHS channel flow moved upward in association with the synchronous activity of the MCT system, triggered intense dehydration of LHS metasediments, resulting in fluid-present melting in both the GHS and LHS during middle to late Miocene, and the formation of leucogranite with mixture features of GHS and LHS. Furthermore, with the cooling of the melt channel, duplexing has gradually operated since the middle to late Miocene in the shallow crust.
High-throughput methods for genome editing: the more the better
Authors: Huang Yong , Shang Meiqi , Liu Tingting , Wang Kejian
Published: January 2022

During the last decade, targeted genome-editing technologies, especially clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) technologies, have permitted efficient targeting of genomes, thereby modifying these genomes to offer tremendous opportunities for deciphering gene function and engineering beneficial traits in many biological systems. As a powerful genome-editing tool, the CRISPR/Cas systems, combined with the development of next-generation sequencing and many other high-throughput techniques, have thus been quickly developed into a high-throughput engineering strategy in animals and plants. Therefore, here, we review recent advances in using high-throughput genome-editing technologies in animals and plants, such as the high-throughput design of targeted guide RNA (gRNA), construction of large-scale pooled gRNA, and high-throughput genome-editing libraries, high-throughput detection of editing events, and high-throughput supervision of genome-editing products. Moreover, we outline perspectives for future applications, ranging from medication using gene therapy to crop improvement using high-throughput genome-editing technologies.
Regulation of ammonium acquisition and use in Oryza longistaminata ramets under nitrogen source heterogeneity
Authors: Kawai Misato , Tabata Ryo , Ohashi Miwa , Honda Haruno , Kamiya Takehiro , Kojima Mikiko , Takebayashi Yumiko , Oishi Shunsuke , Okamoto Satoru , Hachiya Takushi , Sakakibara Hitoshi
Published: January 2022

Oryza longistaminata, a wild rice, vegetatively reproduces and forms a networked clonal colony consisting of ramets connected by rhizomes. Although water, nutrients, and other molecules can be transferred between ramets via the rhizomes, inter-ramet communication in response to spatially heterogeneous nitrogen availability is not well understood. We studied the response of ramet pairs to heterogeneous nitrogen availability using a split hydroponic system that allowed each ramet root to be exposed to different conditions. Ammonium uptake was compensatively enhanced in the sufficient-side root when roots of the ramet pairs were exposed to ammonium-sufficient and ammonium-deficient conditions. Comparative transcriptome analysis revealed that a gene regulatory network for effective ammonium assimilation and amino acid biosynthesis was activated in the sufficient-side roots. Allocation of absorbed nitrogen from the nitrogen-sufficient to the nitrogen-deficient ramets was rather limited. Nitrogen was preferentially used for newly growing axillary buds on the sufficient-side ramets. Biosynthesis of trans-zeatin (tZ), a cytokinin, was upregulated in response to the nitrogen supply, but tZ appeared not to target the compensatory regulation. Our results also implied that the O. longistaminata putative ortholog of rice (Oryza sativa) C-terminally encoded peptide1 plays a role as a nitrogen-deficient signal in inter-ramet communication, providing compensatory upregulation of nitrogen assimilatory genes. These results provide insights into the molecular basis for efficient growth strategies of asexually proliferating plants growing in areas where the distribution of ammonium ions is spatially heterogeneous.
Transcription factor dynamics in plants: Insights and technologies for in vivo imaging
Authors: Zhang Yuan , Lu Yuqing , El Sayyed Hafez , Bian Jiahui , Lin Jinxing , Li Xiaojuan
Published: January 2022

Biochemical and genetic approaches have been extensively used to study transcription factor (TF) functions, but their dynamic behaviors and the complex ways in which they regulate transcription in plant cells remain unexplored, particularly behaviors such as translocation and binding to DNA. Recent developments in labeling and imaging techniques provide the necessary sensitivity and resolution to study these behaviors in living cells. In this review, we present an up-to-date portrait of the dynamics and regulation of TFs under physiologically relevant conditions and then summarize recent advances in fluorescent labeling strategies and imaging techniques. We then discuss future prospects and challenges associated with the application of these techniques to examine TFs’ intricate dance in living plants.
Examining the Spatial Distribution and Contextual Correlates of Body-worn Camera Adoption in the USA
Authors: Lawshe Nathaniel L , Zimmerman Gregory M , Stowell Jacob
Published: January 2022

Body-worn cameras have proliferated in law enforcement agencies over the past decade. Yet, studies examining the relevance of place for body-worn camera adoption are sparse. This study investigated the spatial distribution and contextual correlates of body-worn cameras in the USA. Using data from the 2016, Law Enforcement Management and Administrative Statistics survey linked to the 2016 American Community Survey, this study examined spatial dependence in body-worn camera adoption across US counties and whether higher levels of concentrated disadvantage in an agency’s jurisdiction increased the odds of body-worn camera adoption. LISA (local indicator of spatial association) and colocation maps indicated that counties with body-worn cameras and high levels of concentrated disadvantage were clustered and colocated almost entirely in southern states. Two-level logistic hierarchical models indicated that county-level disadvantage played key roles in predicting body-worn camera adoption. Findings suggest that research should account for the context in which body-worn cameras are adopted.
Are patients’ fears of catching COVID-19 during an emergency hospital admission with an acute urological problem justified?—A UK epicentre experience
Authors: Gbolahan Olayinka , Bonatsos Vasileios , Mukherjee Subhabrata , Raza Asif
Published: January 2022

Background

To establish the risk of catching COVID-19 as urology emergency inpatient and assess patients’ fears and attitudes towards seeking help.

Methods

A single-centre retrospective study of urological emergency admissions was conducted over a 10-week period in 2019 and compared to same period of 2020 pandemic. Also, a telephone survey was performed based on a COVID-19 fear questionnaire.

Results

In-hospital, infection rate was 0.82% during or within 28 days of discharge with no related mortality. The majority of patients were afraid to visit A&E during the pandemic with less being afraid to visit their GPs; 64% were reluctant to seek medical input by trying self-treatment; 56% admitted intentional delay to visit the hospital. Additionally, 56% considered risk of getting COVID-19 was higher rather than leaving their condition untreated. Interestingly, the vast majority (82%) stated that they would not change approach regarding hospital visits if the same situation occurred.

Conclusions

The risk of contracting COVID 19 while a urology in patient in a COVID-19 epicentre was very low with no COVID-19-related mortality. Our data support that patients should be encouraged to attend rather than stay at home during future surges in the pandemic to prevent further non–COVID-19-related harm.
Prevalence of frailty in patients with polymyalgia rheumatica and association with health-related quality of life, cognition and sarcopenia
Authors: Sattui Sebastian E , Jannat-Khah Deanna , Lally Lindsay , Lieber Sarah B , Mandl Lisa A , Spiera Robert F
Published: January 2022

Objective

To describe the prevalence of frailty in a single-centre cohort of patients with PMR and describe its association with health-related quality of life (HRQoL), cognition and sarcopenia.

Methods

This was a cross-sectional study of patients with PMR, according to 2012 EULAR/ACR Classification Criteria, presenting within 12 months of diagnosis and on treatment with glucocorticoids. Frailty was defined according to the Fried frailty criteria. HRQoL was assessed using Patient-Reported Outcomes Measurement Information System Computerized Adaptive Test (PROMIS-CAT) and cognition was assessed using the Mini-Mental State Examination. Sarcopenia was measured by DXA.

Results

Forty-one patients were enrolled. Prevalence of frailty and pre-frailty was 17% and 59%, respectively. Frail patients had higher inflammatory markers at diagnosis compared with pre-frail and robust patients. Of 27 patients with DXA results, 26% were sarcopenic. Frail patients had worse physical function, and more pain behaviour and interference compared with pre-frail and robust patients. In univariable analyses, frail patients were more likely to have worse physical function, and more pain behaviour and pain interference, which remained significant after adjusting for age. There were no significant associations between cognition or sarcopenia and frailty.

Conclusions

In this cohort of PMR patients, there was a higher prevalence of frailty and pre-frailty compared with that reported in community-dwelling elderly. Frailty was associated with worse physical function, and increased pain behaviour and pain interference, differences that were also clinically meaningful. Larger prospective studies are needed to confirm these findings and analyse the association of frailty with other PMR disease outcomes.
Non-criteria manifestations in the presence of antiphospholipid antibodies in a paediatric cohort
Authors: Morán-Álvarez Patricia , Andreu-Suárez África , Caballero-Mota Liz , Gassiot-Riu Susanna , Berrueco-Moreno Rubén , Calzada-Hernández Joan , Antón-López Jordi , Vázquez-Díaz Mónica , Boteanu Alina
Published: January 2022

Objective

To identify the variables associated with the development of non-criteria manifestations in the presence of antiphospholipid antibodies (aPLs) in a paediatric cohort.

Methods

Multicentric historical cohort study of children under the age of 18 years to determine thrombotic events (TEs) and non-criteria manifestations in the presence of aPL.

Results

Eighty-two children were included; 8.5% had at least one TE and 69.5% at least one non-criteria manifestation. Of them, 96.5% did not associate TEs. Haematological manifestations were the most frequent (43.65%), followed by cutaneous (22%), neurological (15.9%) and cardiac (4.9%) events. The most frequent aPLs were: 77.8% LA; 42.7% aCL and 41.5% aβ2GP. The positivity rate was: 64.6% simple, 18.3% double and 17.1% triple. ANA positivity was 68.1%. A bivariate analysis revealed that children with IgM aCL+, IgM aβ2GP+, ANA+, an SLE diagnosis or the absence of TEs had a significantly higher percentage of non-criteria manifestations (P <0.05). The logistic regression showed family history of autoimmune diseases [odds ratio (OR) 4.26, 95% CI: 0.8, 22.2, P =0.086] and the absence of TEs (OR 17.18, 95% CI: 1.2, 244.6, P =0.03) as independent risk factors of developing non-criteria manifestations. An SLE diagnosis, aPL profile and ANA+ were not identified.

Conclusion

Non-criteria manifestations were more frequent than TEs. A positive family history of autoimmune diseases and the absence of TEs were associated with a higher risk of developing non-criteria manifestations. Therefore, their inclusion as APS classification criteria should be considered in order to get an improved prognosis in the paediatric population.
Recent evolution of ancient Arctic leech relatives: systematics of Acanthobdellida
Authors: de Carle Danielle Božena , Gajda Łukasz , Bielecki Aleksander , Cios Stanisław , Cichocka Joanna M , Golden Heidi E , Gryska Andrew D , Sokolov Sergey , Shedko Marina Borisowna , Knudsen Rune , Utevsky Serge , Świątek Piotr , Tessler Michael
Published: January 2022

Acanthobdellida gnaw into the sides of salmonid fishes in frigid Arctic lakes and rivers, latching on with fearsome facial hooks. Sister to leeches, they are an ancient lineage with two described species. Unfortunately, Acanthobdellida are rarely collected, leading to a paucity of literature despite their unique morphology. Populations range from Eurasia to Alaska (USA), but few specimens of Acanthobdella peledina are represented in molecular studies, and no molecular data exist for Paracanthobdella livanowi, making their taxonomic position difficult to assess. We use phylogenetics and morphology to determine whether allopatric populations of A. peledina are distinct species and assess the current classification scheme used for Acanthobdellida. We produce a new suborder, Acanthobdelliformes, to match the taxonomy within Hirudinea. Scanning electron micrographs indicate species-level differences in the anterior sucker and facial hooks; molecular phylogenetics mirrors this divergence between species. We assign both species to the family Acanthobdellidae and abandon the family Paracanthobdellidae. Alaskan and European A. peledina populations are morphologically similar, but appear phylogenetically divergent. Our data strongly suggest that members of the order Acanthobdellida diverged relatively recently in their ancient history, but based on genetic distance, this divergence appears to pre-date the most recent cycles of glaciation.
Intravascular Versus Surface Cooling in Patients Resuscitated From Cardiac Arrest: A Systematic Review and Network Meta-Analysis With Focus on Temperature Feedback
Authors: Ramadanov Nikolai , Arrich Jasmin , Klein Roman , Herkner Harald , Behringer Wilhelm
Published: January 2022

OBJECTIVE:

The aim of the study was to compare the effect of intravascular cooling (IC), surface cooling with temperature feedback (SCF), and surface cooling without temperature feedback (SCnoF) on neurologic outcome and survival in patients successfully resuscitated from cardiac arrest (CA) and treated with targeted temperature management (TTM) at 32–34°C.

DATA SOURCES:

We performed a systematic review on Cochrane Central Register of Controlled Trials, Cochrane Database of Systematic Reviews, MEDLINE, SCOPUS, CINAHL, Web of Science, and Clinical Trials up to June 30, 2021.

STUDY SELECTION:

We included randomized and nonrandomized studies on IC, SCF, and SCnoF in adult humans resuscitated from CA undergoing TTM, reporting neurologic outcome or survival.

DATA EXTRACTION:

We performed a network meta-analysis to assess the comparative effects of IC, SCF, and SCnoF. The overall effect between two cooling methods included the effect of direct and indirect comparisons. Results are given as odds ratios (OR) and 95% CIs. Rankograms estimated the probability of TTM methods being ranked first, second, and third best interventions.

DATA SYNTHESIS:

A total of 14 studies involving 4,062 patients met the inclusion criteria. Four studies were randomized controlled studies, and 10 studies were nonrandomized observational studies. IC compared with SCnoF was significantly associated with better neurologic outcome (OR, 0.6; 95% CI, 0.49–0.74) and survival (OR, 0.8; 95% CI, 0.66–0.96). IC compared with SCF, and SCF compared with SCnoF did not show significant differences in neurologic outcome and survival. The rankogram showed that IC had the highest probability to be the most beneficial cooling method, followed by SCF and SCnoF.

CONCLUSIONS:

Our results suggest that in patients resuscitated from CA and treated with TTM at 32–34°C, IC has the highest probability of being the most beneficial cooling method for survival and neurologic outcome.
Negative association of time in range and urinary albumin excretion rate in patients with type 2 diabetes mellitus: a retrospective study of inpatients
Authors: Chai Sanbao , Wu Shanshan , Xin Sixu , Yuan Ning , Sun Jianbin , Zhang Xiaomei , Ji Linong
Published: January 2022

Background:

Time in range (TIR) refers to the time an individual spends within their target glucose range, which now has been popularized as an important metric to classify glycemic management and also recognized as an important outcome of current diabetes therapies. This study aimed to investigate the association between TIR and the severity of the urinary albumin excretion rate (UAER) in patients with type 2 diabetes mellitus (T2DM).

Methods:

We retrospectively analyzed the data of 1014 inpatients with T2DM at the Department of Endocrinology and Metabolism of Peking University International Hospital, China. TIR was defined as the percentage of blood glucose within the target range of 3.90–10.00 mmol/L. Urine samples for assessment of UAER were collected for 3 consecutive days from the start of hospitalization.

Results:

The TIR values for patients with normal urine levels of albumin, microalbuminuria, and macroalbuminuria were 70% ± 20%, 50% ± 20%, and 30% ± 20%, respectively (all P 
< 0.001). The patients were stratified according to quartiles of TIR as follows: quartile (Q) 1, <55%; Q2, 55%–72%; Q3, 73%–83%; and Q4, >83%. The incidences of microalbuminuria in Q1, Q2, Q3, and Q4 were 41.1%, 21.6%, 7.1%, and 5.5% (all P < 0.001), respectively. The respective incidences of macroalbuminuria were 24.2%, 1.1%, 1.4%, and 0% (all P < 0.001). In multinomial logistic regression analyses, TIR was significantly correlated with microalbuminuria (odds ratio [OR] 0.58, 95% confidence interval [CI]: 0.52–0.65, P < 0.001) and macroalbuminuria (OR 0.26, 95% CI: 0.18–0.38, P < 0.001) after adjusting for age, sex, body mass index, diabetes duration, systolic blood pressure, and levels of triglycerides, glycosylated hemoglobin A1c, and creatinine.

Conclusion:

The proportion of blood glucose in TIR is closely related to the severity of UAER in patients with T2DM.
#Healthcare
Authors: Franklin Kathryn , Engstrand Shannon , Thornton Jason , Connor Jean Anne
Published: January 2022

Background/Introduction

Although social media is becoming a primary resource for information and support in all aspects of life, including health care, limited information is available describing social media use in parents whose child undergoes surgical care.

Objectives/Aims

The aims of this study were to describe how patients/families use social media to address health care needs and understand their perceptions of social media privacy and reliability.

Methods

A descriptive survey of 39 questions, both fixed choice and open ended, was distributed to a convenience sample of parents during their child's preoperative visit. Descriptive statistics were used to summarize fixed-choice responses. Content analysis was used to assess open-ended responses and comments.

Results

A total of 205 completed surveys were available for review. Overall, 195 (95.6%) reported using social media, with 70 (35%) using social media up to 5 times a day and another 61 (30.5%) using it 6 to 40 times a day. Respondents used social media for medical information (122/60.1%), to make health care decisions (53/26.5%), after a diagnosis (104/52%), after a medical visit (88/44%), and to update friends and family (129/65.5%). Most respondents were undecided (111/58.1%) when asked how reliable medical information was on social media sites, with 33 (17.3%) believing medical information to be “reliable to very reliable” on social media sites. Among the 61 comments received, 4 themes emerged: Spectrum of Social Media Use, Social Media and Health Care Interaction, Social Media as a Source of Support and Peer Experience, and Reliability of Social Media.

Discussion

Most respondents utilized social media for health care information while reporting feeling undecided on the reliability of the information. Understanding the multiple ways patients and families utilize social media provides health care members opportunities to discuss medical information, inform health care decision making, and support patient and family needs.
Wedge Defects on Optical Coherence Tomography Angiography of the Peripapillary Retina in Glaucoma: Prevalence and Associated Clinical Factors
Authors: LeTran Vivian H. , Burkemper Bruce , O’Fee John R. , Souverein Erik A. , Lee Jae C. , Phillips Mark J. , Dinh-Dang Duyen , Song Brian J. , Xu Benjamin Y. , Wong Brandon J. , Richter Grace M.
Published: January 2022

Précis:

Among subjects with glaucoma, wedge-shaped defects on optical coherence tomography angiography (OCTA) were associated with disc hemorrhages (DH), paracentral visual field (VF) defects, increased cup-to-disc ratio (CDR), and thinner retinal nerve fiber layer (RNFL).

Purpose:

To examine determinants of wedge defects on peripapillary OCTA in glaucoma.

Materials and Methods:

A total of 278 eyes of 186 subjects with mild to severe primary open-angle glaucoma underwent 6×6 spectral-domain OCTA imaging of the superficial peripapillary retina from 2016 to 2020 at an academic practice. Wedge defects were defined as focal microvasculature loss that extends outward from the optic nerve in an arcuate, wedge shape. Logistic regression models controlling for intereye correlation identified variables significantly associated with wedge defects. Eyes with profound microvasculature loss in both hemispheres were excluded. Candidate variables included: age, sex, race or ethnicity, diabetes, hypertension, follow-up duration, baseline untreated intraocular pressure, intraocular pressure at time of imaging, DH history, paracentral VF defects, CDR, central corneal thickness, spherical equivalent, VF mean deviation, RNFL thickness, and glaucoma stage.

Results:

Of 278 eyes, 126 (45.3%) had wedge defects in at least 1 hemisphere. In our multivariable logistic regression model, wedge defects were associated with DH history [odds ratio (OR): 3.19, 95% confidence interval (CI): 1.05–9.69, P=0.041], paracentral VF defects [OR: 4.38 (95% CI: 2.11–9.11), P<0.0001], larger CDR [OR: 1.27 (95% CI: 1.03–1.56), P=0.024, per 0.1 increase], and thinner RNFL [OR: 1.71 (95% CI: 1.25–2.34), P=0.0009, per 10 μm decrease].

Conclusion:

DH history and paracentral VF defects were independently associated with wedge defects on OCTA, which was present in 45.3% of primary open-angle glaucoma patients. These findings may provide insight into glaucoma pathogenesis.
Pandemics Past, Present, and Future
Authors: Kriebs Jan M.
Published: January 2022

One of the lessons of the current pandemic is that Americans have lost trust in the public health system in the United States (US) and in the health recommendations of the Federal government. History tells us that each pandemic brings new challenges and new lessons. Looking back at the history of pandemics, and at the present experience, nurses and midwives can craft responses to patient concerns and contribute to future planning that better addresses the needs of maternal-child health practices.
Gloving and Touch Practices of Neonatal Nurses
Authors: Nist Marliese Dion , Pickler Rita H. , Harrison Tondi M.
Published: January 2022

Positive tactile experiences in the newborn period are critical to normal sensory development. Universal gloving in the neonatal intensive care unit has become a controversial issue in neonatal nursing practice. Intended to prevent infection among neonatal patients, universal gloving also hinders the provision of human touch. The purpose of this survey study was to (1) describe gloving policies in neonatal intensive care units, and (2) describe the gloving and touch practices of neonatal nurses and identify associations between these practices and demographic characteristics. The investigators developed a 19-question, anonymous survey. The survey link was distributed through the National Association of Neonatal Nurses' social media and newsletter. Of the 137 responses, only 22.1% of nurses reported unit policy requiring universal gloving. While nurses reported some ambiguity about gloving policies, surveyed nurses commonly used gloves when performing general care activities. Institutional gloving policies varied in this geographically diverse sample, but routine, bare-handed touch was an uncommon practice among neonatal nurses. Research evidence is needed to guide nursing practice and inform policy decisions regarding glove use in the neonatal intensive care unit.
The physiology of hemostasis
Authors: Sierra Cristina , Moreno Maite , García-Ruiz Juan C.
Published: January 2022

The breakdown of vascular integrity triggers a series of reactions, initially involving a vasoconstriction phenomenon that reduces blood loss and leads to platelet plug formation under changing flow conditions. Once the platelet plug has formed, the coagulation system is activated in order to allow the formation of fibrin, which anchors the platelet plug to the vessel breach. Finally, once the damage has resolved, the fibrin is eliminated through fibrinolysis. This article explains all these regulatory mechanisms.
Comprehensive lncRNA and mRNA profiles in peripheral blood mononuclear cells derived from ankylosing spondylitis patients by RNA-sequencing analysis
Authors: Li Chuangxin , Qu Wa , Yang Xuefeng
Published: January 2022

The present study aimed to investigate the comprehensive expression profiles of long non-coding RNA (lncRNA) in ankylosing spondylitis (AS).

The peripheral blood samples were collected from 6 AS patients and 6 age- and gender-matched healthy controls (HCs), and separated for peripheral blood mononuclear cells, followed by RNA-sequencing. Further bioinformatics analyses were performed to explore the significantly enriched biological processes, signaling pathways of differentially expressed lncRNAs (DElncRNAs) (based on cis-target and trans-target genes) and differentially expressed mRNAs (DEmRNAs).

Principal component analysis plots indicated that both lncRNA and mRNA expression profiles could distinguish AS patients from HCs; heatmap diagram exhibited a relatively good consistency and tendency of lncRNA and mRNA expression profiles in AS patients and HCs, respectively; volcano plots exhibited 114 upregulated and 45 downregulated DElncRNAs, 284 upregulated and 435 downregulated DEmRNAs in AS patients compared with HCs; Gene ontology enrichment analyses indicated that DElncRNAs (based on cis-target and trans-target genes) and DEmRNAs were enriched in molecular functions (including DNA binding, protein binding, etc) and biological process (including immune response, inflammatory response, etc); Kyoto Encyclopedia of Genes and Genomes enrichment analyses revealed that these DElncRNAs (based on cis-target and trans-target genes) and DEmRNAs were enriched in immune and inflammation-related signaling, such as B cell receptor signaling pathway, TNF signaling pathway, NF-kappa B signaling pathway, etc.

Our study displays the comprehensive expression profiles and functions of lncRNAs involved in AS, which provides reference for further researches discovering candidate lncRNAs with value in assisting early AS diagnosis.
Bipolar hemiarthroplasty may reduce cerebrovascular accidents and improve early weight-bearing in the elderly after femoral neck fracture
Authors: Dubin Jeremy , Atzmon Ran , Feldman Viktor , Farkash Uri , Nyska Meir , Rath Ehud , Palmanovich Esequiel
Published: January 2022

Cerebrovascular accidents (CVA) in the elderly population after femoral neck fracture remain great concern for physicians. Specifically, surgical fixation techniques, such as bipolar hemiarthroplasty (HA) and internal fixation play a significant role in influencing the occurrence of postoperative CVA in the elderly population.

In order to identify 2 cohorts, we used a rigid selection process based on our institution's database. The cohorts were comprised of a HA cohort and a cannulated screw cohort, of which underwent femoral neck surgery, performed by 3 fellowship trained surgeons from 2003 to 2014. Risk factors were documented and measured, including Coumadin use and hypertension, and postoperative complications such as CVA and death rate were also recorded. A P-value of <.05 was determined to be statistically significant.

A power analysis was performed and achieved a power of 0.95. We found a non-significant reduction in CVA for bipolar HA (3.6% CVA vs 0.0% in the non-CVA group, P = .48) and a non-significant increase in CVA for cannulated screw use (7.6% CVA vs 14.4% in the non-CVA group, P = .11). In addition, we found a significant difference in terms of weight-bearing status at 6-weeks postoperatively (0.95 vs 2.0, P < .0001), favoring the bipolar HA group.

Among the advantages of bipolar HA surgery, surgeons should consider its value in reducing the occurrence of postoperative CVA. Furthermore, patients who underwent bipolar HA had improved weight-bearing status postoperatively compared with cannulated screw fixation.
Nebulized fentanyl for respiratory symptoms in patients with COVID-19 (ventanyl trial)
Authors: Shaikh Nissar , Khatib Mohamad Y. , Wraidat Mohammad A. Al , Mohamed Ahmed S. , Al-Assaf Anood A. , Tharayil Abdul Gafoor M. , Abujaber Ahmad A. , Nashwan Abdulqadir J.
Published: January 2022

Patients with coronavirus disease (COVID-19) commonly experience distressing and challenging respiratory symptoms. Interventions such as oxygen therapy, oral opiates, and traditional nebulizers like ipratropium bromide and salbutamol are variable in their efficacy, and therapy responses in patients are difficult to predict. The purpose of this study is to investigate the efficacy of nebulized fentanyl citrate on dyspnea, cough, and throat pain in patients with COVID-19 and evaluate the safety with any potential adverse events.

In COVID-19, about 59% of patients will exhibit cough, 35% generalized body ache and sore throat, and 31% dyspnea. Some methods such as nebulized lidocaine, magnesium sulfate, and systemic opioids have been used to manage the respiratory symptoms. It has been previously shown that fentanyl nebulizer has beneficial effect in improving shortness of breath in patients with chronic obstructive pulmonary disease. The proposed theory behind that was that fentanyl decreased the rate of spontaneous respiratory rate, diminished the brain stem chemoreceptor response to hypoxia and hypercarbia, in addition to exhibiting a modulating effect on the brain stem. Therefore, we hypothesize that nebulized fentanyl has superior effect in improving shortness of breath and relieving cough compared to normal saline, in addition to its advantageous throat pain relief, while exhibiting fewer side effects in patients with COVID 19 infection. Therefore, this phase-III, randomized, comparative, parallel assignment, single-blinded clinical trial aims at assessing the efficacy and safety of nebulized fentanyl to suppress cough, improve breathlessness, and relieve throat pain in patients with COVID-19.
The clinical effectiveness and safety of traditional Chinese medicine Jinfeng pill in adjuvant treatment of infertility with polycystic ovary syndrome
Authors: Zhu Ping , Guan Jing-Zhi , Hai Qing-Chun , Jin Jing , Shi Lei , Hua Lian
Published: January 2022

Background:

Polycystic ovary syndrome (PCOS) is the main cause of infertility in women, the essence of which is an endocrine disorder syndrome with abnormal sugar metabolism and reproductive dysfunction, and the incidence rate of about 6% of women. Traditional Chinese medicine (TCM) Jinfeng pill has achieved very good clinical results in the treatment of infertility with PCOS, but there is currently a lack of strong evidence-based medical evidence. This study uses meta-analysis method to analyze the clinical effectiveness and safety of TCM Jinfeng pill in the treatment of infertility with PCOS, hoping to provide help for the clinical treatment of infertility with PCOS.

Methods:

Using the computer to retrieve SinoMed, CNKI, VIP, WANFANG Database, as well as Public, The Cochrane library, Medline (Ovid SP), Embase and other foreign language databases, while manually retrieving the relevant magazine supplements, special issues, professional materials, network information, and so on. The retrieval time is from the beginning of each database to June 2021. The selected literature is evaluated using the Cochrane System Rating Manual Bias Risk Tool. Statistical analysis and graphics of the inclusion literature are performed using Review Manager 5.3 statistical software.

Results:

All the results of this study on the clinical effectiveness and safety of TCM Jinfeng pill in adjuvant treatment of infertility with PCOS will be published in a peer-reviewed academic journal of medicine.

Ethics and dissemination:

The type of study is systematic evaluation, the whole process of research does not involve human trials, the data used in the institute are obtained through published literature, so ethical review is not suitable for this study.

OSF registration number:

10.17605/OSF.IO/JEP2D. (https://osf.io/jep2d).

Conclusion:

Our research will provide evidence-based medical evidence on whether the TCM Jinfeng pill is effective and safe in the treatment of infertility with PCOS.
The effect of Huoxue Huayu decoction on restenosis after percutaneous coronary intervention in patients with coronary heart disease
Authors: Hou Yi , Li Xuhao , Wang Xingxin , Dong Tiantian , Yang Jiguo
Published: January 2022

Background:

Percutaneous coronary intervention (PCI), as the most common treatment for coronary heart disease (CHD), has the advantages of simple operation, minimal invasion, rapid reconstruction, and vessels opening. The problem, however, is that many patients develop restenosis within 6 months after PCI. In traditional Chinese medicine (TCM), Huoxue Huayu decoction (HXHYD) is widely used to treat cardiovascular diseases, and its important role as a complementary and alternative therapy for the prevention and treatment of post-PCI restenosis in CHD patients has been extensively reported. However, controversy exists among different studies. Therefore, we collected relevant randomized controlled trials for a meta-analysis to assess the efficacy and safety of HXHYD in the prevention of post-PCI restenosis in patients with CHD.

Methods:

Randomized controlled trials of HXHYD in the prevention of post-PCI restenosis in patients with CHD will be retrieved from PubMed, Embase, Web of Science, Cochrane Library, China National Knowledge Infrastructure, Wan Fang Database, Chinese Biomedical Literature Database, VIP Database for Chinese Technical Periodicals, and Clinical Trial Register. The 2 authors will independently conduct the literature search, literature screening, data extraction, and quality assessment. Data analysis will be performed using STATA 14.0.

Results:

The results of this meta-analysis will be submitted to a peer-reviewed journal for publication.

Conclusion:

This study will provide high-quality, evidence-based medical evidence for the efficacy and safety of HXHYD in the prevention of post-PCI restenosis in patients with CHD.

Ethics and dissemination:

Ethical approval is not required for this study. The systematic review will be published in a peer-reviewed journal, presented at conferences, and shared on social media platforms. This review would be disseminated in a peer-reviewed journal or conference presentations.

OSF REGISTRATION NUMBER:

DOI 10.17605/OSF.IO/PNZSM.
Febuxostat combined with hydration for the prevention of contrast-induced nephropathy in hyperuricemia patients undergoing percutaneous coronary intervention
Authors: Ma Guang , Li Min , Teng Wei , He Zhaohui , Zhai Xiaojv , Xia Zhenhua
Published: January 2022

Background:

To assess the efficacy of febuxostat combined with hydration on contrast-induced nephropathy (CIN) in coronary heart disease patients with hyperuricemia undergoing percutaneous coronary intervention (PCI).

Methods:

Patients with hyperuricemia who underwent PCI were randomly assigned to 2 groups. The control group was given hydration only, and the febuxostat group received febuxostat 40 mg daily before administration of contrast agent and hydration. The primary endpoint of the study was the incidence of CIN, defined as an increase in baseline serum creatinine concentration by 25% at 2 days after contrast media administration, and variations in the serum levels of creatinine, neutrophil gelatinase-associated lipocalin, uric acid, and estimated glomerular filtration rate were compared.

Results:

A total of 202 patients with hyperuricemia were randomly assigned to either the febuxostat group (n = 100) or the control group (n = 102). The baseline characteristics of the 2 groups were similar. The incidence of CIN was 6.0% (6/100) in the febuxostat group and 14.71% (15/102) in the control group.

The levels of neutrophil gelatinase-associated lipocalin at 6-hour and serum creatinine and uric acid at 48-hour in the febuxostat combined hydration group were lower than those in the control group after surgery, and the level of estimated glomerular filtration rate was higher than that in the control group (all P < .05). Multivariate logistic regression analysis revealed that febuxostat was an independent predictor of CIN.

Conclusion:

Our study demonstrated that prophylactic treatment with febuxostat combined with hydration can reduce the incidence of CIN in patients with coronary heart disease and hyperuricemia after PCI.
Sublingual immunotherapy for pediatric patients with mite allergies
Authors: Kajiume Teruyuki
Published: January 2022

Sublingual immunotherapy (SLIT) has been increasingly used instead of subcutaneous immunotherapy. SLIT was initially approved for use among adults; however, it has become more widely accepted for children. Few studies have evaluated the effectiveness of SLIT in the treatment of dust mite allergies among children, including adverse effects. This study aimed to investigate the effectiveness of SLIT in children with dust mite allergies, as well as its adverse effects, at a pediatric general outpatient clinic.

I analyzed the data of 181 patients aged 4 to 12 years who tested positive for mite antigen-specific immunoglobulin E, exhibited nasal and/or eye symptoms, and received Miticure. Symptoms were evaluated using the Japanese rhino-conjunctivitis quality of life (QOL) questionnaire no. 1. Wilcoxon tests were used to compare the pretreatment and post-treatment symptom scores. Adverse events were tallied, and Kaplan–Meier curves and Wilcoxon tests were used to assess the proportion of dropouts.

The mean QOL score at the baseline was 2.17 (standard deviation [SD] 1.45). After 1 week, the mean symptom QOL score was 1.63 (SD 1.32); the lowest mean score was found in week 41 (0.48, SD 0.63). A significant decline in the occurrence of all symptoms, including sneezing, nasal discharge, nasal congestion, itchy eyes, and teary eyes, was observed. Adverse effects were observed in 76 (42.0%) patients; the most common adverse effect was itchy mouth.

SLIT improves symptoms with minimal adverse effects in pediatric patients.
A case report of nilotinib-induced irreversible interstitial lung disease
Authors: Cho Jun Yeun , Lee Ok-Jun , Kwon Jihyun , Kim Dohun , Shin Yoon Mi
Published: January 2022

Rationale:

Nilotinib is a second line tyrosine kinase inhibitor to treat patients with chronic myeloid leukemia after imatinib resistance or intolerance. Drug related pulmonary complication is known to be rare. We discuss a case of nilotinib-induced interstitial lung disease presenting with nonspecific interstitial pneumonia on the unilateral lung.

Patient concerns:

A 46-year-old man with chronic-phase chronic myeloid leukemia presented with cough and weight loss for 2 months. He had been treated with nilotinib for 52 months.

Diagnosis:

Computed tomography scan showed right lung dominant consolidations, ground glass opacities and traction bronchiectasis. Bronchoalveolar lavage fluid analysis revealed no evidence of infection or malignancy. Surgical lung biopsy specimen was consistent with fibrosing nonspecific interstitial pneumonia. The patient was diagnosed with nilotinib induced interstitial lung disease.

Interventions:

Corticosteroid treatment was initiated with prednisolone (50 mg daily) and slowly tapered down for 2 months.

Outcomes:

Cough improved after the course of corticosteroid treatment. However, fibrotic lung lesions persisted. Reinitiation of nilotinib resulted in the worsening of lung lesions.

Lessons:

We report a case of irreversible interstitial lung disease that caused by nilotinib. Clinicians should have suspicion of this potential pulmonary complication in patients with respiratory symptoms and abnormal radiologic findings during nilotinib treatment, albeit rarely.