bluuebunny/crossref_metadata_2025 huggingface dataset:
Matryoshka-Type Liposomes Offer the Improved Delivery of Temoporfin to Tumor Spheroids
Authors: Yakavets Ilya , Millard Marie , Lamy Laureline , Francois Aurelie , Scheglmann Dietrich , Wiehe Arno , Lassalle Henri-Pierre , Zorin Vladimir , Bezdetnaya Lina
Published: September 2019

The balance between the amount of drug delivered to tumor tissue and the homogeneity of its distribution is a challenge in the efficient delivery of photosensitizers (PSs) in photodynamic therapy (PDT) of cancer. To date, many efforts have been made using various nanomaterials to efficiently deliver temoporfin (mTHPC), one of the most potent photosensitizers. The present study aimed to develop double-loaded matryoshka-type hybrid nanoparticles encapsulating mTHPC/cyclodextrin inclusion complexes in mTHPC-loaded liposomes. This system was expected to improve the transport of mTHPC to target tissues and to strengthen its accumulation in the tumor tissue. Double-loaded hybrid nanoparticles (DL-DCL) were prepared, characterized, and tested in 2D and 3D in vitro models and in xenografted mice in vivo. Our studies indicated that DL-DCL provided deep penetration of mTHPC into the multicellular tumor spheroids via cyclodextrin nanoshuttles once the liposomes had been destabilized by serum proteins. Unexpectedly, we observed similar PDT efficiency in xenografted HT29 tumors for liposomal mTHPC formulation (Foslip®) and DL-DCL.
Towards Improved Hybrid Actuation Mechanisms for Flapping Wing Micro Air Vehicles: Analytical and Experimental Investigations
Authors: Hassanalian Mostafa , Abdelkefi Abdessattar
Published: September 2019

A new strategy is proposed in order to effectively design the components of actuation mechanisms for flapping wing micro air vehicles. To this end, the merits and drawbacks of some existing types of conventional flapping actuation mechanisms are first discussed qualitatively. Second, the relationships between the design of flapping wing actuation mechanism and the entrance requirements including the upstroke and downstroke angles and flapping frequency are determined. The effects of the components of the actuation mechanism on the kinematic and kinetic parameters are investigated. It is shown that there are optimum values for different parameters in order to design an efficient mechanism. Considering the optimized features for an actuation mechanism, the design, analysis, and fabrication of a new hybrid actuation mechanism for FWMAV named “Thunder I” with fourteen components consisting of two six-bar mechanisms are performed. The results show that this designed hybrid actuation mechanism has high symmetrical flapping motion with hinged connections for all components. The proposed methodology for the modeling and fabrication of Thunder I’s actuation mechanism can be utilized as guidelines to design efficient FWMAVs actuation mechanisms.
Empirical Study on the Efficiency of an LPG-Supplied Range Extender for Electric Vehicles
Authors: Lasocki Jakub , Kopczyński Artur , Krawczyk Paweł , Roszczyk Paweł
Published: September 2019

A range extender is an auxiliary power unit, usually consisting of an internal combustion engine and an electric generator, which is used to charge a battery of an electric vehicle in order to increase its range. This paper considers a range extender supplied with liquefied petroleum gas (LPG). The aim is to provide detailed data on thermal efficiency, brake specific fuel consumption (BSFC), and unit emission of carbon dioxide (CO2) in a broad spectrum of range extender operating conditions defined by rotational speed and torque. The experimental investigation has been conducted using a laboratory test stand equipped with an energy dissipation system of adjustable resistance. Measurement results, including fuel flow rate, were processed using custom algorithm for generating maps, i.e., two-dimensional dependencies of the considered parameters on the rotational speed and torque. The maps obtained for LPG supply were compared with those for gasoline supply. The results demonstrated feasibility of LPG-supplied range extender. Its BSFC and thermal efficiency were at a comparable level to those obtained for gasoline supply, but with less CO2 emission. The empirical data collected has been adopted in the simulation of extended-range electric vehicle in a driving cycle, showing the potential of utilizing the results of this study.
Autochthonous and Probiotic Lactic Acid Bacteria Employed for Production of “Advanced Traditional Cheeses”
Authors: Fusco Vincenzina , Quero Grazia , Poltronieri Palmiro , Morea Maria , Baruzzi Federico
Published: September 2019

Microbial characterization of two Italian traditional cheeses, Giuncata and Caciotta Leccese, was carried out, with the aim to isolate autochthonous bacterial strains to be used as starters to improve and standardize the quality of these cheeses. More than 400 bacterial isolates were found, using PCR-based identification, to belong to 12 species of the Streptococcus, Lactococcus, Lactobacillus, and Leuconostoc genera. The dominant strains were screened for antagonistic activity against pathogenic and spoilage bacteria and exopolysaccharide production, acidification, and proteolytic activity. Since Streptococcus macedonicus was found to be the most prevalent lactic acid bacteria species present in milk and in both types of cheese, the best performing strain of this species was successfully used, alone or in combination with a selected autochthonous Lactococcus lactis strain, in pilot-scale productions of Giuncata and Caciotta Leccese cheeses, respectively. The combined inoculums of selected autochthonous strains positively influenced the sensory characteristics of both Giuncata and Caciotta cheeses. Finally, the selected autochthonous cultures were enriched with a potentially probiotic Lactobacillus rhamnosus strain and successfully used in pilot-scale productions of these traditional cheeses. To the best of our knowledge, this is the first study reporting the use of an autochthonous S. macedonicus strain as a starter for the production of cheeses with added probiotics. In addition, the identification of the probiotic strain in the feces of healthy volunteers fed with the advanced traditional cheese proved its effectiveness as a carrier for the delivery of probiotics to the human body.
The Influence of Transactive Memory System on Individual Career Resilience: The Role of Taking Charge and Self-Promotion
Authors: Liu Yuhao , Zhou Xingchi , Liao Shudi , Liao Jianqiao , Guo Zhiwen
Published: September 2019

The transactive memory system is known as an effective group cognitive system as well as a knowledge-sharing structure for organizations to keep competitive advantages in today’s dynamic and knowledge-based business environment. However, its influence at the individual level remains vague. The purpose of this study is to explore the influence of a transactive memory system (TMS) on individual career resilience through the theoretical perspective of conservation of resources theory (COR). This research proposes and examines a moderated mediation model that elaborates how a transactive memory system affects individual career resilience. A two-stage empirical study was conducted among 328 employees from companies in China. The findings suggest that a transactive memory system significantly influences individual career resilience positively, and employee taking-charge behavior plays a mediating role in that relationship. Furthermore, the results supported our moderated mediation model, which indicates that individuals with high self-promotion motives are more likely to engage in taking-charge behavior than those with low self-promotion motives, and the former reported higher career resilience than the latter eventually. Theoretical and practical implications are also provided in the discussion section.
Universal or Targeted Antenatal Care for Immigrant Women? Mapping and Qualitative Analysis of Practices in Denmark
Authors: Villadsen Sarah Fredsted , Ims Hodan Jama , Nybo Andersen Anne-Marie
Published: September 2019

Inequity in immigrants’ health during pregnancy and childbirth has been shown. We studied the Danish regional organization of public midwifery-based antenatal care (ANC) for immigrant women to assess the strengths and weaknesses of organizing ANC as either universal or immigrant-targeted. A telephone survey in 2012 to all the Danish maternity wards (n = 20) was conducted. Semi-structured interviews with midwives providing targeted care (n = 6) were undertaken and characteristics of care were qualitatively analyzed, having the immigrant density of the facilities, the Danish ANC policy, and theories of cultural competence as the frame of reference. Six maternity wards were providing immigrant-targeted ANC. Targeted care implied longer consultations and increased attention to the individual needs of immigrant women. At these facilities, navigation in the health care system, body awareness, and use of interpreter services were key topics. The selection of women for targeted care was based on criteria (including names) that risk stigmatizing immigrant women. The arguments for not providing targeted care included that immigrant-targeted care was considered stigmatizing. Current universal care may overlook the needs of immigrant women and contribute to inequities. A strategy could be to improve dynamic cultural competencies of midwives, interpreter services, and flexibility of the care provision of the universal ANC system.
Cadmium (Cd) and Nickel (Ni) Distribution on Size-Fractioned Soil Humic Substance (SHS)
Authors: Hsieh Sheng-Hsien , Chiu Teng-Pao , Huang Wei-Shiang , Chen Ting-Chien , Yeh Yi-Lung
Published: September 2019

Soil humic substances (SHS) are heterogeneous, complex mixtures, whose concentration, chemical composition, and structure affect the transport and distribution of heavy metals. This study investigated the distribution behavior of two heavy metals [cadmium (Cd) and nickel (Ni)] in high molecular weight SHS (HMHS, 1 kDa–0.45 μm) and low molecular weight SHS (LMHS, <1 kDa) extracted from agricultural soils. The HMHS mass fractions were 45.1 ± 19.3%, 17.1 ± 6.7%, and 57.7 ± 18.5% for dissolved organic carbon (DOC), Cd, and Ni, respectively. The metal binding affinity, unit organic carbon binding with heavy metal ratios ([Me]/[DOC]), were between 0.41 ± 0.09 μmol/g-C and 7.29 ± 2.27 μmol/g-C. Cd preferred binding with LMHS (p < 0.001), while Ni preferred binding with HMHS (p < 0.001). The optical indicators SUVA254, SR, and FI were 3.16 ± 1.62 L/mg-C/m, 0.54 ± 0.18 and 1.57 ± 0.15, respectively for HMHS and 2.65 ± 1.25 L/mg-C/m, 0.40 ± 0.17, and 1.68 ± 0.12, respectively for LMHS. The HMHS contained more aromatic and lower FI values than LMHS. Multilinear regression showed a significant positive correlation between the measured predicted [Me]/[DOC] ratios (r = 0.52–0.72, p < 0.001). The results show that the optical indices can distinguish the chemical composition and structure of different size SHS and predict the binding ability of Me-SHS.
Leg Dominance as a Risk Factor for Lower-Limb Injuries in Downhill Skiers—A Pilot Study into Possible Mechanisms
Authors: Promsri Arunee , Longo Alessia , Haid Thomas , Doix Aude-Clémence M. , Federolf Peter
Published: September 2019

Leg dominance has been reported as one potential risk factor for lower-limb injuries in recreational downhill skiers. The current study proposed and tested two possible mechanisms for a leg dominance effect on skiing injuries—imbalance of the knee muscle strength and bilateral asymmetry in sensorimotor control. We hypothesized that the knee muscle strength (Hypothesis 1; H1) or postural control (Hypothesis 2; H2) would be affected by leg dominance. Fifteen well-experienced recreational downhill skiers (aged 24.3 ± 3.2 years) participated in this study. Isometric knee flexor/extensor muscle strength was tested using a dynamometer. Postural control was explored by using a kinematic principal component analysis (PCA) to determine the coordination structure and control of three-dimensional unipedal balancing movements while wearing ski equipment on firm and soft standing surfaces. Only H2 was supported when balancing on the firm surface, revealing that when shifting body weight over the nondominant leg, skiers significantly changed the coordination structure (p < 0.006) and the control (p < 0.004) of the lifted-leg movements. Based on the current findings, bilateral asymmetry in sensorimotor control rather than asymmetry in strength seems a more likely mechanism for the previously reported effect of leg dominance on lower-limb injury risk in recreational downhill skiers.
Direct or Spillover Effect: The Impact of Pure Technical and Scale Efficiencies of Water Use on Water Scarcity in China
Authors: Li Min , Long Kaisheng
Published: September 2019

The spatial relationship between water use efficiency and water scarcity has been widely discussed, but little attention has been paid to the impact of the pure technical and scale efficiencies of water use on water scarcity. Using input-oriented data envelopment analysis (DEA) and panel spatial Durbin models (SDM), the direct and spillover effects of different water use efficiencies on water scarcity from 2007 to 2016 in China were examined at the regional scale. The results show that the water use pure technical efficiency had significantly negative direct effects on water scarcity; however, the water use scale efficiency did not have a similar effect. The improvement in water use pure technical efficiency in one region could aggravate the water scarcity in neighboring regions through spatial spillover effects, but the same effect was not observed between the water use scale efficiency and water scarcity. Finally, we propose solutions to improve the water use efficiency to reduce the water scarcity.
5G Wireless Communication and Health Effects—A Pragmatic Review Based on Available Studies Regarding 6 to 100 GHz
Authors: Simkó Myrtill , Mattsson Mats-Olof
Published: September 2019

The introduction of the fifth generation (5G) of wireless communication will increase the number of high-frequency-powered base stations and other devices. The question is if such higher frequencies (in this review, 6–100 GHz, millimeter waves, MMW) can have a health impact. This review analyzed 94 relevant publications performing in vivo or in vitro investigations. Each study was characterized for: study type (in vivo, in vitro), biological material (species, cell type, etc.), biological endpoint, exposure (frequency, exposure duration, power density), results, and certain quality criteria. Eighty percent of the in vivo studies showed responses to exposure, while 58% of the in vitro studies demonstrated effects. The responses affected all biological endpoints studied. There was no consistent relationship between power density, exposure duration, or frequency, and exposure effects. The available studies do not provide adequate and sufficient information for a meaningful safety assessment, or for the question about non-thermal effects. There is a need for research regarding local heat developments on small surfaces, e.g., skin or the eye, and on any environmental impact. Our quality analysis shows that for future studies to be useful for safety assessment, design and implementation need to be significantly improved.
Relevance of Surface Neuronal Protein Autoantibodies as Biomarkers in Seizure-Associated Disorders
Authors: Stanciu Gabriela Dumitrita , Bild Veronica , Ababei Daniela Carmen , Rusu Razvan Nicolae , Beschea Chiriac Sorin Ioan , Rezuş Elena , Luca Andrei
Published: September 2019

The detection of neuronal surface protein autoantibody-related disorders has contributed to several changes in our understanding of central nervous system autoimmunity. The clinical presentation of these disorders may be associated (or not) with tumors, and often patients develop an inexplicable onset of epilepsy, catatonic or autistic features, or memory and cognitive dysfunctions. The autoantigens in such cases have critical roles in synaptic transmission and plasticity, memory function, and process learning. For months, patients with such antibodies may be comatose or encephalopathic and yet completely recover with palliative care and immunotherapies. This paper reviews several targets of neuronal antibodies as biomarkers in seizure disorders, focusing mainly on autoantibodies, which target the extracellular domains of membrane proteins, namely leucine-rich glioma-inactivated-1 (LGI1), contactin-associated protein-like 2 (CASPR2), the N-methyl-D-aspartate receptor (NMDAR), γ-aminobutyric acid receptor-B (GABABR), the glycine receptor (GlyR), and a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs). In order to restore health status, limit hospitalization, and optimize results, testing these antibodies should be done locally, using internationally certified procedures for a precise and rapid diagnosis, with the possibility of initiating therapy as soon as possible.
Reaction-Based, Fluorescent Film Deposition from Dopamine and a Diamine-Tethered, Bis–Resorcinol Coupler
Authors: Alfieri Maria Laura , Iacomino Mariagrazia , Napolitano Alessandra , d’Ischia Marco
Published: September 2019

The reaction-based deposition on various surfaces of an all-organic fluorescent coating is reported here, involving autoxidation of 2 mM dopamine in carbonate buffer at pH 9.0, in the presence of a 1 mM diamine–resorcinol coupler (Bis–Res) prepared from 2,4-dihydroxybenzaldehyde and hexamethylenediamine (HMDA). Spectral analysis of the films coupled with an LC-MS investigation of the yellow fluorescent mixture was compatible with the formation and deposition of HMDA-linked methanobenzofuroazocinone fluorophores. Both the emission properties and hydrophobicity of the film were abated in a reversible manner following exposure to acid vapors. These results provide an entry to efficient and practical fluorescent coating methodologies based on in situ generation and the deposition of wet adhesive, as well as fluorescent materials combining a strongly emitting fluorophore with the film-forming properties of long chain diamines.
Circulating miRNA Profiling in Plasma Samples of Ovarian Cancer Patients
Authors: Penyige András , Márton Éva , Soltész Beáta , Szilágyi-Bónizs Melinda , Póka Róbert , Lukács János , Széles Lajos , Nagy Bálint
Published: September 2019

Ovarian cancer is one of the most common cancer types in women characterized by a high mortality rate due to lack of early diagnosis. Circulating miRNAs besides being important regulators of cancer development could be potential biomarkers to aid diagnosis. We performed the circulating miRNA expression analysis in plasma samples obtained from ovarian cancer patients stratified into FIGO I, FIGO III, and FIGO IV stages and from healthy females using the NanoString quantitative assay. Forty-five miRNAs were differentially expressed, out of these 17 miRNAs showed significantly different expression between controls and patients, 28 were expressed only in patients, among them 19 were expressed only in FIGO I patients. Differentially expressed miRNAs were ranked by the network-based analysis to assess their importance. Target genes of the differentially expressed miRNAs were identified then functional annotation of the target genes by the GO and KEGG-based enrichment analysis was carried out. A general and an ovary-specific protein–protein interaction network was constructed from target genes. Results of our network and the functional enrichment analysis suggest that besides HSP90AA1, MYC, SP1, BRCA1, RB1, CFTR, STAT3, E2F1, ERBB2, EZH2, and MET genes, additional genes which are enriched in cell cycle regulation, FOXO, TP53, PI-3AKT, AMPK, TGFβ, ERBB signaling pathways and in the regulation of gene expression, proliferation, cellular response to hypoxia, and negative regulation of the apoptotic process, the GO terms have central importance in ovarian cancer development. The aberrantly expressed miRNAs might be considered as potential biomarkers for the diagnosis of ovarian cancer after validation of these results in a larger cohort of ovarian cancer patients.
ProTAME Arrest in Mammalian Oocytes and Embryos Does Not Require Spindle Assembly Checkpoint Activity
Authors: Radonova Lenka , Svobodova Tereza , Skultety Michal , Mrkva Ondrej , Libichova Lenka , Stein Paula , Anger Martin
Published: September 2019

In both mitosis and meiosis, metaphase to anaphase transition requires the activity of a ubiquitin ligase known as anaphase promoting complex/cyclosome (APC/C). The activation of APC/C in metaphase is under the control of the checkpoint mechanism, called the spindle assembly checkpoint (SAC), which monitors the correct attachment of all kinetochores to the spindle. It has been shown previously in somatic cells that exposure to a small molecule inhibitor, prodrug tosyl-l-arginine methyl ester (proTAME), resulted in cell cycle arrest in metaphase, with low APC/C activity. Interestingly, some reports have also suggested that the activity of SAC is required for this arrest. We focused on the characterization of proTAME inhibition of cell cycle progression in mammalian oocytes and embryos. Our results show that mammalian oocytes and early cleavage embryos show dose-dependent metaphase arrest after exposure to proTAME. However, in comparison to the somatic cells, we show here that the proTAME-induced arrest in these cells does not require SAC activity. Our results revealed important differences between mammalian oocytes and early embryos and somatic cells in their requirements of SAC for APC/C inhibition. In comparison to the somatic cells, oocytes and embryos show much higher frequency of aneuploidy. Our results are therefore important for understanding chromosome segregation control mechanisms, which might contribute to the premature termination of development or severe developmental and mental disorders of newborns.
Linkage of Periodontitis and Rheumatoid Arthritis: Current Evidence and Potential Biological Interactions
Authors: de Molon Rafael Scaf , Rossa Jr. Carlos , Thurlings Rogier M. , Cirelli Joni Augusto , Koenders Marije I.
Published: September 2019

The association between rheumatoid arthritis (RA) and periodontal disease (PD) has been the focus of numerous investigations driven by their common pathological features. RA is an autoimmune disease characterized by chronic inflammation, the production of anti-citrullinated proteins antibodies (ACPA) leading to synovial joint inflammation and destruction. PD is a chronic inflammatory condition associated with a dysbiotic microbial biofilm affecting the supporting tissues around the teeth leading to the destruction of mineralized and non-mineralized connective tissues. Chronic inflammation associated with both RA and PD is similar in the predominant adaptive immune phenotype, in the imbalance between pro- and anti-inflammatory cytokines and in the role of smoking and genetic background as risk factors. Structural damage that occurs in consequence of chronic inflammation is the ultimate cause of loss of function and disability observed with the progression of RA and PD. Interestingly, the periodontal pathogen Porphyromonas gingivalis has been implicated in the generation of ACPA in RA patients, suggesting a direct biological intersection between PD and RA. However, more studies are warranted to confirm this link, elucidate potential mechanisms involved, and ascertain temporal associations between RA and PD. This review is mainly focused on recent clinical and translational research intends to discuss and provide an overview of the relationship between RA and PD, exploring the similarities in the immune-pathological aspects and the possible mechanisms linking the development and progression of both diseases. In addition, the current available treatments targeting both RA and PD were revised.
Calcium Mechanisms in Limb-Girdle Muscular Dystrophy with CAPN3 Mutations
Authors: Lasa-Elgarresta Jaione , Mosqueira-Martín Laura , Naldaiz-Gastesi Neia , Sáenz Amets , López de Munain Adolfo , Vallejo-Illarramendi Ainara
Published: September 2019

Limb-girdle muscular dystrophy recessive 1 (LGMDR1), previously known as LGMD2A, is a rare disease caused by mutations in the CAPN3 gene. It is characterized by progressive weakness of shoulder, pelvic, and proximal limb muscles that usually appears in children and young adults and results in loss of ambulation within 20 years after disease onset in most patients. The pathophysiological mechanisms involved in LGMDR1 remain mostly unknown, and to date, there is no effective treatment for this disease. Here, we review clinical and experimental evidence suggesting that dysregulation of Ca2+ homeostasis in the skeletal muscle is a significant underlying event in this muscular dystrophy. We also review and discuss specific clinical features of LGMDR1, CAPN3 functions, novel putative targets for therapeutic strategies, and current approaches aiming to treat LGMDR1. These novel approaches may be clinically relevant not only for LGMDR1 but also for other muscular dystrophies with secondary calpainopathy or with abnormal Ca2+ homeostasis, such as LGMD2B/LGMDR2 or sporadic inclusion body myositis.
Effects of a Resident Yeast from the Honeybee Gut on Immunity, Microbiota, and Nosema Disease
Authors: Tauber James P. , Nguyen Vy , Lopez Dawn , Evans Jay D.
Published: September 2019

The western honeybee (Apis mellifera) has a core bacterial microbiota that is well described and important for health. Honeybees also host a yeast community that is poorly understood with respect to host nutrition and immunity, and also the symbiotic bacterial microbiota. In this work, we present two studies focusing on the consequences of dysbiosis when honeybees were control-fed a yeast that was isolated from a honeybee midgut, Wickerhamomyces anomalus. Yeast augmentation for bees with developed microbiota appeared immunomodulatory (lowered immunity and hormone-related gene expression) and affected the microbial community, while yeast augmentation for newly emerged bees without an established bacterial background did not lead to decreased immunity— and hormone—related gene expression. In newly emerged bees that had a naturally occurring baseline level of W. anomalus, we observed that the addition of N. ceranae led to a decrease in yeast levels. Overall, we show that yeasts can affect the microbiome, immunity, and physiology.
Impact of Disease-Specific Fears on Pulmonary Rehabilitation Trajectories in Patients with COPD
Authors: Janssens Thomas , Van de Moortel Zora , Geidl Wolfgang , Carl Johannes , Pfeifer Klaus , Lehbert Nicola , Wittmann Michael , Schultz Konrad , von Leupoldt Andreas
Published: September 2019

Disease-specific fears predict health status in chronic obstructive pulmonary disease (COPD), but their role in pulmonary rehabilitation (PR) remains poorly understood and especially longer-term evaluations are lacking. We therefore investigated changes in disease-specific fears over the course of PR and six months after PR, and investigated associations with PR outcomes (COPD assessment test (CAT) and St. Georges respiratory questionnaire (SGRQ)) in a subset of patients with COPD (n = 146) undergoing a 3-week inpatient PR program as part of the STAR study (Clinicaltrials.gov, ID: NCT02966561). Disease-specific fears as measured with the COPD anxiety questionnaire improved after PR. For fear of dyspnea, fear of physical activity and fear of disease progression, improvements remained significant at six-month follow-up. Patients with higher disease-specific fears at baseline showed elevated symptom burden (CAT and SGRQ Symptom scores), which persisted after PR and at follow-up. Elevated disease-specific fears also resulted in reduced improvements in Quality of Life (SGRQ activity and impact scales) after PR and at follow-up. Finally, improvement in disease-specific fears was associated with improvement in symptom burden and quality of life. Adjustment for potential confounding variables (sex, smoking status, age, lung function, and depressive symptoms) resulted in comparable effects. These findings show the role of disease-specific fears in patients with COPD during PR and highlight the need to target disease-specific fears to further improve the effects of PR.
Silk-Based Therapeutics Targeting Pseudomonas aeruginosa
Authors: McKay Tina B. , Parker Rachael N. , Hawker Morgan J. , McGill Meghan , Kaplan David L.
Published: September 2019

Pseudomonas aeruginosa (P. aeruginosa) infections may lead to severe damage of the cornea, mucosa, and skin. The highly aggressive nature of P. aeruginosa and the rise in multi-drug resistance, particularly in nosocomial settings, lead to an increased risk for permanent tissue damage and potentially death. Thus, a growing need exists to develop alternative treatments to reduce both the occurrence of bacterial infection and biofilm development, as well as pathological progression post-infection. Silk derived from Bombyx mori silkworms serves as a unique biomaterial that is biocompatible with low immunogenicity and high versatility, and thereby ideal for stabilizing therapeutics. In this study, we assessed the cytotoxicity of P. aeruginosa on human corneal stromal stem cells and two mucosal cell lines (Caco-2 and HT29-MTX). To determine whether antibiotic-immobilized scaffolds can serve as alternative therapeutics to free, diffuse forms, we developed novel gentamicin-conjugated silk films as functional scaffolds and compared antimicrobial effects and free gentamicin. The advantages of generating a surface coating with a covalently-bound antibiotic may reduce potential side-effects associated with free gentamicin, as well as limit the diffusion of the drug. Our results suggest that gentamicin conjugated to native silk and carboxyl-enriched silk inhibits P. aeruginosa growth. Development of stabilized antibiotic treatments with surface toxicity selective against bacteria may serve as an alternative approach to treat active infections, as well as potential prophylactic use as coatings in high-risk cases, such as post-surgical complications or prolonged hospitalization.
OSSEC IDS Extension to Improve Log Analysis and Override False Positive or Negative Detections
Authors: Teixeira Diogo , Assunção Leonardo , Pereira Teresa , Malta Silvestre , Pinto Pedro
Published: September 2019

Intrusion Detection Systems (IDS) are used to prevent attacks by detecting potential harmful intrusion attempts. Currently, there are a set of available Open Source IDS with different characteristics. The Open Source Host-based Intrusion Detection System (OSSEC) supports multiple features and its implementation consists of Agents that collect and send event logs to a Manager that analyzes and tests them against specific rules. In the Manager, if certain events match a specific rule, predefined actions are triggered in the Agents such as to block or unblock a particular IP address. However, once an action is triggered, the systems administrator is not able to centrally check and obtain detailed information of the past event logs. In addition, OSSEC may assume false positive or negative detections and their triggered actions: previously harmless but blocked IP addresses by OSSEC have to be unblocked in order to reestablish normal operation or potential harmful IP addresses not previously blocked by OSSEC should be blocked in order to increase protection levels. These operations to override OSSEC actions must be manually performed in every Agent, thus requiring time and human resources. Both these limitations have a higher impact on large scale OSSEC deployments assuming tens or hundreds of Agents. This paper proposes an extension to OSSEC that improves the administrator analysis capability by maintaining, organizing and presenting Agent logs in a central point, and it allows for blocking or unblocking IP addresses in order to override actions triggered by false detections. The proposed extension aims to increase efficiency of time and human resources management, mainly considering large scale OSSEC deployments.
Partitioning of Interstitial Segregants during Decohesion: A DFT Case Study of the Σ3 Symmetric Tilt Grain Boundary in Ferritic Steel
Authors: Huang Xiang , Janisch Rebecca
Published: September 2019

The effect of hydrogen atoms at grain boundaries in metals is usually detrimental to the cohesion of the interface. This effect can be quantified in terms of the strengthening energy, which is obtained following the thermodynamic model of Rice and Wang. A critical component of this model is the bonding or solution energy of the atoms to the free surfaces that are created during decohesion. At a grain boundary in a multicomponent system, it is not immediately clear how the different species would partition and distribute on the cleaved free surfaces. In this work, it is demonstrated that the choice of partitioning pattern has a significant effect on the predicted influence of H and C on grain boundary cohesion. To this end, the Σ 3 ( 112 ) [ 1 1 ¯ 0 ] symmetric tilt grain boundary in bcc Fe with different contents of interstitial C and H was studied, taking into account all possible distributions of the elements, as well as surface diffusion effects. H as a single element has a negative influence on grain boundary cohesion, independent of the details of the H distribution. C, on the other hand, can act both ways, enhancing or reducing the cohesion of the interface. The effect of mixed H and C compositions depends on the partition pattern. However, the general trend is that the number of detrimental cases increases with increasing H content. A decomposition of the strengthening energy into chemical and mechanical contributions shows that the elastic contribution dominates at high C contents, while the chemical contribution sets the trend for high H contents.
Stability and Bifurcation of a Delayed Time-Fractional Order Business Cycle Model with a General Liquidity Preference Function and Investment Function
Authors: Xie Yingkang , Wang Zhen , Meng Bo
Published: September 2019

In this paper, the business cycle (BC) is described by a delayed time-fractional-order model (DTFOM) with a general liquidity preference function and an investment function. Firstly, the existence and uniqueness of the DTFOM solution are proven. Then, some conditions are presented to guarantee that the positive equilibrium point of DTFOM is locally stable. In addition, Hopf bifurcation is obtained by a new method, where the time delay is regarded as the bifurcation parameter. Finally, a numerical example of DTFOM is given to verify the effectiveness of the proposed model and methods.
A New Look for the Red Macroalga Palmaria palmata: A Seafood with Polar Lipids Rich in EPA and with Antioxidant Properties
Authors: Lopes Diana , Melo Tânia , Meneses Joana , Abreu Maria H. , Pereira Rui , Domingues Pedro , Lillebø Ana I. , Calado Ricardo , Domingues M. Rosário
Published: September 2019

Palmaria palmata is an edible red macroalga widely used for human consumption and valued for its high protein value. Despite its low total lipid content, it is rich in eicosapentaenoic acid (EPA). This seaweed has been scarcely explored with regard to its lipid composition. The polar lipids of seaweeds are nowadays recognized as important phytochemicals contributing to their add value valorization and providing support for claims of potential health benefits. The present study aimed to disclose the polar lipid profile of P. palmata, farmed in an integrated multi-trophic aquaculture (IMTA) through modern lipidomic approaches using high-resolution LC-MS and MS/MS and to screen for the antioxidant properties of this red macroalga. A total of 143 molecular species of lipids were identified, belonging to several classes of polar lipids, such as glycolipids, phospholipids, and betaine lipids. It is noteworthy that the most abundant lipid species in each class were esterified with eicosapentaenoic acid (EPA), accounting for more than 50% of the lipid content. The polar lipid extract rich in EPA showed antioxidant activity with an inhibition concentration (IC) of IC30 = 171 ± 19.8 µg/mL for α,α-diphenyl-β-picrylhydrazyl radical (DPPH●) and IC50 = 26.2 ± 0.1 µg/mL for 2,20-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radical cation (ABTS●+). Overall, this study highlights that P. palmata farmed in an IMTA framework can be a sustainable source of beneficial lipids with antioxidant activity. Moreover, this red macroalga can be exploited for future applications as a source of lipids rich in EPA for food and feed, nutraceuticals, and cosmetics.
The Architecture of Monospecific Microalgae Biofilms
Authors: Fanesi Andrea , Paule Armelle , Bernard Olivier , Briandet Romain , Lopes Filipa
Published: September 2019

Microalgae biofilms have been proposed as an alternative to suspended cultures in commercial and biotechnological fields. However, little is known about their architecture that may strongly impact biofilm behavior, bioprocess stability, and productivity. In order to unravel the architecture of microalgae biofilms, four species of commercial interest were cultivated in microplates and characterized using a combination of confocal laser scanning microscopy and FTIR spectroscopy. In all the species, the biofilm biovolume and thickness increased over time and reached a plateau after seven days; however, the final biomass reached was very different. The roughness decreased during maturation, reflecting cell division and voids filling. The extracellular polymeric substances content of the matrix remained constant in some species, and increased over time in some others. Vertical profiles showed that young biofilms presented a maximum cell density at 20 μm above the substratum co-localized with matrix components. In mature biofilms, the maximum density of cells moved at a greater distance from the substratum (30–40 μm), whereas the maximum coverage of matrix components remained in a deeper layer. Carbohydrates and lipids were the main macromolecules changing during biofilm maturation. Our results revealed that the architecture of microalgae biofilms is species-specific. However, time similarly affects the structural and biochemical parameters.
Food-Intake Normalization of Dysregulated Fatty Acids in Women with Anorexia Nervosa
Authors: Nguyen Nhien , Dow Michelle , Woodside Blake , German J. Bruce , Quehenberger Oswald , Shih Pei-an Betty
Published: September 2019

Anorexia nervosa (AN) is a psychiatric disorder affected by psychological, environmental, and biological factors. Individuals with AN avoid high-fat, high-calorie diets and have shown abnormal metabolism of fatty acids (FAs), which are essential for brain and cognitive/neuropsychiatric health. To clarify the relationship between FAs and AN, fasting and postprandial plasma FAs in AN patients and age-matched control women were analyzed via mass-spectrometry. Clinical phenotypes were assessed using Becker Anxiety Inventory and Becker Depression Inventory. AN patients and controls exhibited different FA signatures at both fasting and postprandial timepoints. Lauric acid, eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), and alpha-linoleic acid (ALA) were higher in AN than in controls (lauric acid: 15,081.6 ± 14,970.2 vs. 8257.4 ± 4740.2 pmol/mL; ALA at fasting: 2217.7 ± 1587.6 vs. 1087.9 ± 821.2 pmol/mL; ALA at postprandial: 1830.9 ± 1115.6 vs. 1159.4 ± 664.7 pmol/mL. EPA: 33,788.3 ± 17,487.5 vs. 22,860.6 ± 12,642.4 pmol/mL; DPA: 32,664.8 ± 16,215.0 vs. 20,969.0 ± 12,350.0 pmol/mL. FDR-adjusted p-values < 0.05). Food intake and AN status modified the correlations of FAs with body mass index (BMI), depression, and anxiety. Desaturases SCD-18 and D6D showed lower activities in AN compared to controls. Altered FA signature, specifically correlations between elevated n-3 FAs and worsened symptoms, illustrate metabolic underpinnings in AN. Future studies should investigate the mechanisms by which FA dysregulation, specifically elevated n-3 FAs, affects AN risk and outcome.
A Role for Nanoparticles in Treating Traumatic Brain Injury
Authors: Bony Badrul Alam , Kievit Forrest
Published: September 2019

Traumatic brain injury (TBI) is one of the main causes of disability in children and young adults, as well as a significant concern for elderly individuals. Depending on the severity, TBI can have a long-term impact on the quality of life for survivors of all ages. The primary brain injury can result in severe disability or fatality, and secondary brain damage can increase the complexities in cellular, inflammatory, neurochemical, and metabolic changes in the brain, which can last decades post-injury. Thus, survival from a TBI is often accompanied by lifelong disabilities. Despite the significant morbidity, mortality, and economic loss, there are still no effective treatment options demonstrating an improved outcome in a large multi-center Phase III trial, which can be partially attributed to poor target engagement of delivered therapeutics. Thus, there is a significant unmet need to develop more effective delivery strategies to overcome the biological barriers that would otherwise inhibit transport of materials into the brain to prevent the secondary long-term damage associated with TBI. The complex pathology of TBI involving the blood-brain barrier (BBB) has limited the development of effective therapeutics and diagnostics. Therefore, it is of great importance to develop novel strategies to target the BBB. The leaky BBB caused by a TBI may provide opportunities for therapeutic delivery via nanoparticles (NP). The focus of this review is to provide a survey of NP-based strategies employed in preclinical models of TBI and to provide insights for improved NP based diagnostic or treatment approaches. Both passive and active delivery of various NPs for TBI are discussed. Finally, potential therapeutic targets where improved NP-mediated delivery could increase target engagement are identified with the overall goal of providing insight into open opportunities for NP researchers to begin research in TBI.
Understanding the Role of the Antioxidant System and the Tetrapyrrole Cycle in Iron Deficiency Chlorosis
Authors: Santos Carla S. , Ozgur Rengin , Uzilday Baris , Turkan Ismail , Roriz Mariana , Rangel António O.S.S. , Carvalho Susana M.P. , Vasconcelos Marta W.
Published: September 2019

Iron deficiency chlorosis (IDC) is an abiotic stress often experienced by soybean, owing to the low solubility of iron in alkaline soils. Here, soybean lines with contrasting Fe efficiencies were analyzed to test the hypothesis that the Fe efficiency trait is linked to antioxidative stress signaling via proper management of tissue Fe accumulation and transport, which in turn influences the regulation of heme and non heme containing enzymes involved in Fe uptake and ROS scavenging. Inefficient plants displayed higher oxidative stress and lower ferric reductase activity, whereas root and leaf catalase activity were nine-fold and three-fold higher, respectively. Efficient plants do not activate their antioxidant system because there is no formation of ROS under iron deficiency; while inefficient plants are not able to deal with ROS produced under iron deficiency because ascorbate peroxidase and superoxide dismutase are not activated because of the lack of iron as a cofactor, and of heme as a constituent of those enzymes. Superoxide dismutase and peroxidase isoenzymatic regulation may play a determinant role: 10 superoxide dismutase isoenzymes were observed in both cultivars, but iron superoxide dismutase activity was only detected in efficient plants; 15 peroxidase isoenzymes were observed in the roots and trifoliate leaves of efficient and inefficient cultivars and peroxidase activity levels were only increased in roots of efficient plants.
Parametrically Amplified Low-Power MEMS Capacitive Humidity Sensor
Authors: Likhite Rugved , Banerjee Aishwaryadev , Majumder Apratim , Karkhanis Mohit , Kim Hanseup , Mastrangelo Carlos H.
Published: September 2019

We present the design, fabrication, and response of a polymer-based Laterally Amplified Chemo-Mechanical (LACM) humidity sensor based on mechanical leveraging and parametric amplification. The device consists of a sense cantilever asymmetrically patterned with a polymer and flanked by two stationary electrodes on the sides. When exposed to a humidity change, the polymer swells after absorbing the analyte and causes the central cantilever to bend laterally towards one side, causing a change in the measured capacitance. The device features an intrinsic gain due to parametric amplification resulting in an enhanced signal-to-noise ratio (SNR). Eleven-fold magnification in sensor response was observed via voltage biasing of the side electrodes without the use of conventional electronic amplifiers. The sensor showed a repeatable and recoverable capacitance change of 11% when exposed to a change in relative humidity from 25–85%. The dynamic characterization of the device also revealed a response time of ~1 s and demonstrated a competitive response with respect to a commercially available reference chip.
Sustainable Urban Innovation Strategies
Authors: Mulder Karel F.
Published: September 2019

In recent years, it has become a commonplace to argue that cities should be the focus point of sustainable development [...]
Evaluation of MOD16 Algorithm over Irrigated Rice Paddy Using Flux Tower Measurements in Southern Brazil
Authors: Souza Vanessa de Arruda , Roberti Débora Regina , Ruhoff Anderson Luis , Zimmer Tamíres , Adamatti Daniela Santini , Gonçalves Luis Gustavo G. de , Diaz Marcelo Bortoluzzi , Alves Rita de Cássia Marques , Moraes Osvaldo L. L. de
Published: September 2019

Evapotranspiration (ET) is an important component of the hydrological cycle. Understanding the ET process has become of fundamental importance given the scenario of global change and increasing water use, especially in the agricultural sector. Determining ET over large agricultural areas is a limiting factor due to observational data availability. In this regard, remote sensing data has been used to estimate ET. In this study, we evaluated the Moderate-Resolution Imaging Spectroradiometer (MODIS) land surface ET product estimates (hereafter MOD16 ET – MODIS Global Terrestrial Evapotranspiration Product) over two rice paddy areas in Southern Brazil, through the ET measured using the eddy covariance technique (hereafter EC). The energy balance components were evaluated during fallow and flooded seasons showing latent heat flux dominates in both seasons. The results showed that MOD16 ET underestimated EC measurements. Overall, the RMSE (root mean square error) ranged between 13.40 and 16.35 mm 8-day−1 and percent bias (PBIAS) ranged between −33.7% and −38.7%. We also assessed the ET (measured and estimated) main drivers, with EC yielding higher correlation against observed net radiation (Rn) and global radiation (Rg), followed by air temperature (Temp) and vapor pressure deficit (VPD), whilst MOD16 ET estimates yielded higher correlation against leaf area index (LAI) and fraction of photosynthetically active radiation (fPAR). The MOD16 algorithm was forced with meteorological measurements but the results did not improve as expected, suggesting a low sensitivity to meteorological inputs. Our results indicated when a water layer was present over the soil surface without vegetation (LAI around zero), the largest differences between EC measurements and MOD16 ET were found. In this period, the expected domain of soil evaporation was not observed in MOD16 ET physical processes partition, indicating the algorithm was not able to detect areas with high soil moisture. In general, the MOD16 ET product presented low accuracy when compared against experimental measurements over flooded rice paddy, suggesting more studies are necessary, in order to reduce uncertainties associated to the land cover conditions.