bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of Drought-Resistant Genes in Shanlan Upland Rice
Authors: Niu Xiaoling , Zhai Nanxin , Yang Xinsen , Su Meng , Liu Caiyue , Wang Liu , Qu Pengzheng , Liu Wuge , Yuan Qianhua , Pei Xinwu
Published: January 2022

Shanlan upland rice is a kind of upland rice and is suitable for planting in the mountains and in hilly terrain. It is mainly found in China’s Hainan province. To discover the drought-resistant genes in Shanlan upland rice, two representative varieties—Baishanuo (BSN) and Dongfang Manpoxiang (MPX)—were selected for transcriptome sequencing, after which gene expression analysis was used to confirm their gene expression patterns. The results demonstrated that 2791 and 829 differentially expressed genes (DEGs) were identified for each variety, including 184 and 58 transcriptional factors, respectively. Expression analysis demonstrated that some genes with unknown functions, such as Os10g0505900, were highly expressed under drought stress treatment. The transcriptomic data and digital gene expression profiling data obtained in this study provide a basis for studying the drought-resistant mechanism in Shanlan upland rice.
Structure and Function of the Soil Rhizosphere Fungal Communities in Medicinal Plants—A Preliminary Study
Authors: Applebaum Itaii , Jeyaraman Mareeswaran , Sherman Chen , Doniger Tirza , Steinberger Yosef
Published: January 2022

Plants regulate their rhizosphere microbiome, which partly comprises the fungal community. We conducted a study in order to determine the effect that five medicinal plant species (Origanum syriacum, Salvia fruticosa, Teucrium capitatum, Myrtus communis and Pistacia lentiscus) have on the fungal community in their rhizosphere. We measured abiotic parameters and used sequencing to determine the structure of the rhizosphere fungal community, both taxonomically, as phyla and genera, and functionally, as trophic modes. Our data shows that the rhizosphere fungal communities were significantly different, both taxonomically and functionally. The rhizosphere of M. communis had a significant relative abundance of saprotrophs and a lower relative abundance of symbiotrophs than the control soil and the rhizosphere of T. capitatum. The relative abundance of the genus Aureobasidium was significantly higher in the rhizosphere of P. lentiscus than in the control and for all other rhizospheres, but that of S. fruiticosa. The relative abundance of genus Alternaria was lower in the rhizospheres of S. fruticosa and M. communis than in the control soil. Our results highlight the potential use of these plants in agroforestry, as a means to influence the soil fungi population.
Active-Modified Atmosphere Packaging of Ready-to-Eat Pomegranate (Punica granatum L.) Arils at Ambient Temperature for Extending Shelf-Life
Authors: Rokalla Preethi , Inbaraj Baskaran Stephen , Dikkala Praveen Kumar , Sridhar Kandi , Dasi Daniel Smith , Koka Lalitha , Munakala Ramalakshmi , Galipothula Ranjith , Chelli Kavitha Swarupa Rani , Kalletlapally Naveen Kumar
Published: January 2022

Modified atmosphere packaging (MAP) has been widely applied to extend the shelf-life of fresh-cut produces, such as ready-to-eat pomegranate arils. However, many studies used MAP to extend the shelf-life of arils at 3, 4, and 15 °C. The evidence suggested that MAP can extend the shelf-life of arils at an ambient temperature. Therefore, we attempted to extend the shelf-life of ready-to-eat pomegranate arils using active MAP at an ambient temperature and evaluated its chemical, quality, and microbial properties. Samples were flushed with specific gas compositions of oxygen (O2) and carbon dioxide (CO2) at an ambient temperature and 60 ± 2% relative humidity (RH). The findings demonstrated that active MAP increased the titratable acidity (0.32–3.64%), anthocyanins (0.27–0.42 g/L), reducing sugars (1.06–10.13%), and total soluble solids (15–19 °Brix), while it decreased the pH (3.20–3.54) and hardness (4.60–9.40 N) with a microbial load of ≤5 log CFU/g at ambient temperature for 5 days. Ultimately, active MAP could be an effective technique to be used in ready-to-eat produce industries and has the potential to guarantee a longer shelf-life at ambient temperature without the use of refrigerator facilities.
Influence of Pre-Hydrolysis on the Chemical Composition of Prunus avium Cherry Seeds
Authors: Cruz-Lopes Luísa , Dulyanska Yuliya , Domingos Idalina , Ferreira José , Fragata Anabela , Guiné Raquel , Esteves Bruno
Published: January 2022

During the industrial processing of sweet cherry fruits, the seeds are considered agricultural waste and must be disposed of, typically through burning. In this context, it is intended to contribute to the scientific development of the ecovalorization of by-products and to provide new strategies for their transformation into value-added products obtained from sweet cherry seeds (SCS). This work aimed to establish the chemical characterization of SCS before and after several pre-hydrolysis steps in order to allow the solubilization of hemicelluloses that can later be used for the recovery of sugars. The higher percentage of cellulose and lignin remaining in the solid phase will allow its further processing for an integral valorization of the raw material. The temperature (160 and 170 °C) and time (0 and 180 min) of pre-hydrolysis were optimized to obtain the best liquefaction. The percentage of liquefied material was determined from the solid waste obtained at the time of filtration. The best liquefaction by the hydrolysis of SCS was obtained at 170 °C and 180 min, with a yield of 26.7%. The chemical analyses of SCS throughout hydrolysis showed the solubilization of hemicelluloses with increases in the time and temperature of the reactor. α-cellulose and lignin showed an increase both with temperature and time, increasing the material’s potential for further processing in adhesives. FTIR analysis showed that there were significant changes in the spectra between the initial SCS, the solid residue, and the liquefied material. Pre-hydrolysis was proven to be an efficient process to improve the chemical composition of the material for further processing into adhesives or higher-mechanical-strength polyurethane foams.
Soil Sustainability: Analysis of the Soil Compaction under Heavy Agricultural Machinery Traffic in Extensive Crops
Authors: Botta Guido Fernando , Nardon Gustavo Fabian , Guirado Clavijo Rafael
Published: January 2022

Crop establishment depends mostly on the soil preparation and sowing methods used. Our main goal was to evaluate soil compaction and its effects on wheat (Triticum aestivum L.) and soybean (Glycine max L.) yields and seedling emergence with two different tillage methods: no-tillage (NT) and conventional tillage (CT). The study was done in the Western Pampas Region during three cropping seasons. The soil of the study site is a Mollisol. The variables measured were: (1) cone index (CI), (2) dry bulk density (DBD), (3) seedling emergence (SE), and (4) crops yield (CY). For both crops, seedling emergence was slower in NT than in CT, but results were similar 22 days after sowing. After 3 years, the results show that in NT the DBD and CI reached values of 1653 kg m−3 and 3210 kPa, respectively (between 275 and 300 mm). While in CT the values of DBD and CI reached were 1540 kg m−3 and 2300 kPa respectively at the same depth. The highest yields were found in CT (3.31 and 4.10 tons/ha−1, for soybean and wheat, respectively) compared to NT (2.91 and 3.53 tons/ha−1). Topsoil horizon has to be tilled to improve crop yields. In spite of the high number of equipment passes in CT, both tillage systems caused subsoil compaction.
Root Growth and Architecture of Wheat and Brachypodium Vary in Response to Algal Fertilizer in Soil and Solution
Authors: Mau Lisa , Junker Simone , Bochmann Helena , Mihiret Yeshambel E. , Kelm Jana M. , Schrey Silvia D. , Roessner Ute , Schaaf Gabriel , Watt Michelle , Kant Josefine , Arsova Borjana
Published: January 2022

Alternative, recycled sources for mined phosphorus (P) fertilizers are needed to sustain future crop growth. Quantification of phenotypic adaptations and performance of plants with a recycled nutrient source is required to identify breeding targets and agronomy practices for new fertilization strategies. In this study, we tested the phenotypic responses of wheat (Triticum aestivum) and its genetic model, Brachypodium (Brachypodium distachyon), to dried algal biomass (with algae or high or low mineral P) under three growing conditions (fabricated ecosystems (EcoFABs), hydroponics, and sand). For both species, algal-grown plants had similar shoot biomass to mineral-grown plants, taking up more P than the low mineral P plants. Root phenotypes however were strongly influenced by nutrient form, especially in soilless conditions. Algae promoted the development of shorter and thicker roots, notably first and second order lateral roots. Root hairs were 21% shorter in Brachypodium, but 24% longer in wheat with algae compared to mineral high P. Our results are encouraging to new recycled fertilization strategies, showing algae is a nutrient source to wheat and Brachypodium. Variation in root phenotypes showed algal biomass is sensed by roots and is taken up at a higher amount per root length than mineral P. These phenotypes can be selected and further adapted in phenotype-based breeding for future renewal agriculture systems.
Screening of Wheat (Triticum aestivum L.) Genotypes for Drought Tolerance through Agronomic and Physiological Response
Authors: Ahmad Ali , Aslam Zubair , Javed Talha , Hussain Sadam , Raza Ali , Shabbir Rubab , Mora-Poblete Freddy , Saeed Tasbiha , Zulfiqar Faisal , Ali Muhammad Moaaz , Nawaz Muhammad , Rafiq Muhammad , Osman Hany S. , Albaqami Mohammed , Ahmed Mohamed A. A. , Tauseef Muhammad
Published: January 2022

Water scarcity is a major challenge to wheat productivity under changing climate conditions, especially in arid and semi-arid regions. During recent years, different agronomic, physiological and molecular approaches have been used to overcome the problems related to drought stress. Breeding approaches, including conventional and modern breeding, are among the most efficient options to overcome drought stress through the development of new varieties adapted to drought. Growing drought-tolerant wheat genotypes may be a sustainable option to boost wheat productivity under drought stress conditions. Therefore, the present study was conducted with the aim to screen different wheat genotypes based on stress tolerance levels. For this purpose, eleven commonly cultivated wheat genotypes (V1 = Akbar-2019, V2 = Ghazi-2019, V3 = Ujala-2016, V4 = Zincol-2016, V5 = Anaj-2017, V6 = Galaxy-2013, V7 = Pakistan-2013, V8 = Seher-2006, V9 = Lasani-2008, V10 = Faisalabad-2008 and V11 = Millat-2011) were grown in pots filled with soil under well-watered (WW, 70% of field capacity) and water stress (WS, 35% of field capacity) conditions. Treatments were arranged under a completely randomized design (CRD) with three replicates. Data on yield and yield-related traits (tillers/plant, spikelets/spike, grains/spike, 100 grain weight, seed and biological yield) and physio-biochemical (chlorophyll contents, relative water content, membrane stability index, leaf nitrogen, phosphorus, and potassium content) attributes were recorded in this experiment. Our results showed that drought stress significantly affected the morpho-physiological, and biochemical attributes in all tested wheat varieties. Among the genotypes, all traits were found to be significantly (p < 0.05) higher in wheat genotype Faisalabad-2008, including biological yield (9.50 g plant−1) and seed yield (3.39 g plant−1), which was also proven to be more drought tolerant than the other tested genotypes. The higher biological and grain yield of genotype Faisalabad-2008 was mainly attributed to greater numbers of tillers/plant and spikelets/spike compared to the other tested genotypes. The wheat genotype Galaxy-2013 had significantly lower biological (7.43 g plant−1) and seed yield (2.11 g plant−1) than all other tested genotypes, and was classified as a drought-sensitive genotype. For the genotypes, under drought stress, biological and grain yield decreased in the order V10 > V2 > V1 > V4 > V7 > V11 > V9 > V8 > V3 > V6. These results suggest that screening for drought-tolerant genotypes may be a more viable option to minimize drought-induced effects on wheat in drought-prone regions.
A Review on Alaria alata, Toxoplasma gondii and Sarcocystis spp. in Mammalian Game Meat Consumed in Europe: Epidemiology, Risk Management and Future Directions
Authors: Guardone Lisa , Armani Andrea , Mancianti Francesca , Ferroglio Ezio
Published: January 2022

Game meat is increasingly appreciated and consumed in Europe, also due to the growing population of wild ungulates. In addition to interesting nutritional properties and market opportunities, game meat is characterized by some specific public health issues. This review focuses on the etiology, epidemiology, public health aspects and risk management along the supply chain, including parasite detection at slaughtering and inactivation in meat, of three selected foodborne parasitic hazards (Alaria alata, Toxoplasma gondii and Sarcocystis spp.) in the main mammalian game meat species in the EU: wild boar (Sus scrofa), red deer (Cervus elaphus), roe deer (Capreolus capreolus), fallow deer (Dama dama), Alpine chamois (Rupicapra rupicapra), moose (Alces alces), hare (Lepus europaeus) and wild rabbit (Oryctolagus cuniculus). The presented data point out the main issues, and knowledge gaps as well as the potential for improved control in order to contribute to the risk analysis process. To pursue an effective management of these parasitic zoonoses, awareness raising should involve all figures in the supply chain, including hunters, restaurateurs and consumers. Human behaviour and the lack of knowledge regarding meat borne parasitic zoonoses and the health risks they pose seem to be the most important factors responsible for human infections. However, detection methods, starting from the sampling procedure, should be further developed and standardized in order to improve the collection of accurate and up-to-date epidemiological data.
A Case Study Using Accelerometers to Identify Illness in Ewes following Unintentional Exposure to Mold-Contaminated Feed
Authors: Gurule Sara C. , Flores Victor V. , Forrest Kylee K. , Gifford Craig A. , Wenzel John C. , Tobin Colin T. , Bailey Derek W. , Hernandez Gifford Jennifer A.
Published: January 2022

Sensor technologies can identify modified animal activity indicating changes in health status. This study investigated sheep behavior before and after illness caused by mold-contaminated feed using tri-axial accelerometers. Ten ewes were fitted with HerdDogg biometric accelerometers. Five ewes were concurrently fitted with Axivity AX3 accelerometers. The flock was exposed to mold-contaminated feed following an unexpected ration change, and observed symptomatic ewes were treated with a veterinarian-directed protocol. Accelerometer data were evaluated 4 days before exposure (d −4 to −1); the day of ration change (d 0); and 4 days post exposure (d 1 to 4). Herddogg activity index correlated to the variability of minimum and standard deviation of motion intensity monitored by the Axivity accelerometer. Herddogg activity index was lower (p < 0.05) during the mornings (0800 to 1100 h) of days 2 to 4 and the evening of day 1 than days −4 to 0. Symptomatic ewes had lower activity levels in the morning and higher levels at night. After accounting for symptoms, activity levels during days 1 to 4 were lower (p < 0.05) than days −4 to 0 the morning after exposure. Results suggest real-time or near-real time accelerometers have potential to detect illness in ewes.
Potential Owner-Related Risk Factors That May Contribute to Obesity in Companion Dogs in Aotearoa New Zealand
Authors: Forrest Rachel , Awawdeh Leena , Esam Fiona , Pearson Maria , Waran Natalie
Published: January 2022

Approximately a third of all Aotearoa New Zealand (NZ) households include a dog, with 28% of these dogs being overweight or obese, conditions that are associated with many serious health issues. Therefore, healthy weight interventions that focus on the owner’s role are of great importance to companion animal welfare in NZ. Accordingly, the present study explores the feeding practices associated with NZ dogs and identifies potential owner-related risk factors contributing to these animals being overweight or obese. The current study used data collected from a survey conducted online in 2019 between January and March of NZ residents over 18. Along with demographic questions, the respondents were asked questions regarding their dog’s body condition and diet questions related to the body. Nearly a quarter (26%, n = 609) of the survey participants (n = 2358) owned at least one dog. The current study reported that increasing age range, household income and the number of children increased the likelihood of having a dog while increasing qualification level and living in a town/city decreased the likelihood. The majority of the respondents fed their dog(s) treats (59%) and 85% fed them specialised food bought from a pet shop, veterinary clinic and/or different online sources. Just over a third of the participant (39%) reported that they fed their dog(s) biscuits from the supermarket, 36% fed their dog(s) raw meat, and 34% of respondents fee their dog(s) table scraps/human food. These results suggest that many dog owners feed their dog(s) various food types, making it a challenging task to determine the exact amount required from each type in order not to exceed caloric intake. Disagreement regarding the correct body condition were reported among twenty per cent of the respondents. This finding indicates a knowledge gap among the NZ dog-owning population that may negatively affect their dogs’ welfare and wellbeing. Future research into pro-equity approaches to address these issues is needed so that dogs in NZ can live not only a good life but also their best life.
Out of Sight, Out of Mind or Just Something in the Way? Visual Barriers Do Not Reduce Intraspecific Agonism in an All-Male Group of Nile Crocodiles (Crocodylus niloticus)
Authors: Leeds Austin , Riley Alex , Terry Megan , Mazorra Marcus , Wick Lindsay , Krug Scott , Wolfe Kristen , Leonard Ike , Daneault Andy , Alba Andrew C. , Miller Angela , Soltis Joseph
Published: January 2022

Here, we evaluated if visual barriers could reduce intraspecific agonism in an all-male group of Nile crocodiles (Crocodylus niloticus) living in a zoo. Crocodiles were monitored for nearly 100 h, and four “hotspots” of aggression within their exhibit were identified. Within these four locations, visual barriers were placed at the surface of the water with the goal of reducing agonism by targeting sight lines associated with their species-typical minimum exposure posture, where crocodiles submerge their body but maintain facial sensory organs above the water line. Crocodile behavior was then monitored for 226 h, evaluating both short- and long-term effects of the visual barriers. In both observation periods, intraspecific agonism was unaffected by visual barriers. However, crocodiles were more likely to be on land and closer together, after the barriers were installed, showing the barriers affected nonagonistic behaviors. Monitoring of such unintended effects is significant to ensure no welfare concerns are created in any exhibit or husbandry modification attempt. Additionally, time of day and temperature were significant predictors of behavior, highlighting the importance of such factors in the analysis of reptilian behavior. While ineffective at reducing agonism, this is the first published study evaluating exhibit design and behavior of crocodilians in zoos and aquariums. The methodologies and findings here should provide useful information for future behavioral and welfare studies of this understudied taxa.
Jojoba Seed Oil as Feed Additive for Sustainable Broiler Meat Production under Hot Climatic Conditions
Authors: Abdel-Wareth Ahmed A. A. , Mobashar Muhammad , Shah Anwar , Sadiq Abu Bakkar
Published: January 2022

This study aimed to evaluate the impact of dietary addition of jojoba seed oil on productive performance, physicochemical attributes and carcass quality of broiler meat under tropical weather conditions. A total of 384 one-day-old Ross-308 were subdivided into four dietary treatments of jojoba seed oil: 0, 50, 100 and 150 mg/kg of control diet. Each treatment group included twelve replicates with eight birds each. The results showed that dietary supplementation of jojoba seed oil linearly increased (p < 0.01) feed intake, body weight gain and improved (p < 0.01) feed conversion ratio. Interestingly, diets supplemented with jojoba seed oil linearly (p < 0.05) improved the percentage of dressing and reduced abdominal fat percentage compared to the control group. Dietary supplementation of jojoba seed oil showed no effects (p ≥ 0.05) on the weight of internal organs, including liver, heart, gizzard, spleen and pancreas of broiler chickens. Increasing jojoba seed oil levels in the diet decreased (p < 0.001) percentages of cook and drip losses of breast and leg (drumstick and thigh) muscles of broilers. It was concluded that jojoba seed oil used as a feed additive up to 150 mg/kg improves growth performance and meat quality of broiler chickens in tropical weather conditions.
The Effects of Replacing Soybean Meal with Rapeseed Meal, Cottonseed Cake, and Fava Beans on the Milk Yield and Quality Traits in Milking Ewes
Authors: Kalogianni Aphrodite I. , Moschovas Marios , Chrysanthakopoulou Foteini , Lazou Thomai , Theodorou Georgios , Politis Ioannis , Bossis Ioannis , Gelasakis Athanasios I.
Published: January 2022

The replacement of soybean meal (SBM) from intensively reared dairy sheep diets has emerged as a significant challenge for sustainable production. However, the effects of this replacement on milk production have not been sufficiently elucidated. The objective of this study was to prospectively assess the effects of replacing SBM with a mixture of alternative protein sources on the milk yield (MY) and the milk quality traits (MQT) in intensively reared dairy sheep. A total of 112 multiparous, purebred milking ewes of the Chios and Frizarta breeds, from two intensive dairy sheep farms, were involved in the study, postweaning, and were assigned to either the control (CR) or the experimental ration (ER) group. In the ER, 3/4 of the SBM was replaced by a mixture of rapeseed meal, cottonseed cake, and fava beans, producing a ration of a similar nutritional value. MY, MQT, and body condition scores were recorded for each individual ewe monthly for a period of 4 months during lactation. The experimental ration was associated with beneficial effects on daily and 100-day fat yields and on the electrical conductivity of milk as an improved udder health status indicator, with no adverse effects on any of the rest of the studied milk production traits.
Serum Oxytocin in Cows Is Positively Correlated with Caregiver Interactions in the Impossible Task Paradigm
Authors: D’Aniello Biagio , Mastellone Vincenzo , Pinelli Claudia , Scandurra Anna , Musco Nadia , Tudisco Raffaella , Pero Maria Elena , Infascelli Federico , Di Lucrezia Alfredo , Lombardi Pietro
Published: January 2022

This study explored a possible relationship between the circulating oxytocin, cortisol, and the willingness of dairy cows to engage in social behaviors with humans in an experimental context. The behaviors of twenty-nine cows were recorded during the impossible task paradigm, a procedure aimed at creating a violation of expectancy, in the presence of the caregiver and a stranger. The results showed that serum oxytocin levels were positively correlated with duration and negatively correlated with the latency of the cows’ social interactions with the caregiver. This research provides a clear correlation between circulating oxytocin and a willingness to engage in social contact with the caregiver, excluding the possible effect of different cortisol levels on such behavior.
Temporal Partitioning between Forest-Dwelling Small Rodents in a Mediterranean Deciduous Woodland
Authors: Viviano Andrea , Scarfò Manuel , Mori Emiliano
Published: January 2022

Temporal partitioning is reported as one of the main strategies adopted by coexisting mammal species to limit interspecific competition and behavioural interference. In the last decades, camera-trapping surveys have provided valuable insights in assessing temporal niche and activity rhythms of medium and large-sized mammalian species. Conversely, this method has been poorly applied to small rodents. In this work we aimed at assessing temporal niche partitioning between two species of forest-dwelling small rodents—Apodemus flavicollis and Clethrionomys glareolus—by means of intensive camera-trapping. Camera traps were placed in areas where previous genetic analyses have confirmed the only presence of A. flavicollis amongst wood mice species, to prevent misinterpretation of records. We collected 124 independent records of A. flavicollis and 67 records of C. glareolus over three years. The former was mostly nocturnal, with activity peaking after midnight, whereas the latter was mostly active at dawn and dusk. Therefore, a limited temporal overlap was observed, confirming the potential for interspecific competition. Intraguild interference competition between A. flavicollis and C. glareolus may play a pivotal role forcing C. glareolus to be more active in daylight hours where, the more strictly nocturnal A. flavicollis is present. Nocturnal activity of C. glareolus was limited and not influenced by moon phases, whereas A. flavicollis was mostly active in the darkest nights, avoiding bright moonlight nights.
Impacts of Rearing Enrichments on Pullets’ and Free-Range Hens’ Positive Behaviors across the Flock Cycle
Authors: Campbell Dana L. M. , Belson Sue , Dyall Tim R. , Lea Jim M. , Lee Caroline
Published: January 2022

Enrichment during the indoor rearing of pullets destined for free-range systems may optimize pullet development including increasing motivated natural behaviors (termed ‘positive behaviors’) including foraging, dust bathing and chick play. Hy-Line Brown® chicks (n = 1700) were floor-reared indoors across 16 weeks with three enrichment treatments (n = 3 pens/treatment): (1) standard control, (2) weekly novel objects—‘novelty’, (3) perching/navigation structures—‘structural’. At 16 weeks, pullets (n = 1386) were transferred to nine identical pens within rearing treatments with outdoor range access from 25 to 65 weeks. Video cameras recorded the pullet pens, adult indoor pens, and outside range. During rearing, observations of play behavior (running, frolicking, wing-flapping, sparring) in chicks at 2, 4 and 6 weeks (total of 432 thirty-second scans: 16 observations × 3 days × 9 pens) showed no overall effect of rearing treatment (p = 0.16). At 11 and 14 weeks only the ‘novelty’ hens were observed to increase their foraging across age (p = 0.009; dust bathing: p = 0.40) (total of 612 thirty-second scans per behavior: 17 observations × 2 days × 2 age points × 9 pens). Observations of adult hens at 26, 31, 41, 50, 60 and 64 weeks showed that the structural hens exhibited overall more dust bathing and foraging than the control hens (both p < 0.04) but both novelty and/or structural hens showed small increases depending on the behavior and location (total of 4104 scans per behavior: 17 observations × 2 days × 6 age points × 9 pens × 2 locations = 3672 + an additional 432 observations following daylight saving). Across age, adult hens differed in the degree of dust bathing performed inside or outside (both p ≤ 0.001) and foraging outside (p < 0.001) but not inside (p = 0.15). For litter-reared pullets, additional enrichments may result in some long-term increases in positive behaviors.
Antibiotic Stewardship in Retail Pharmacies and the Access-Excess Challenge in China: A Policy Review
Authors: Zhang Tingting , Lambert Helen , Zhao Linhai , Liu Rong , Shen Xingrong , Wang Debin , Cabral Christie
Published: January 2022

In China, efforts to restrict excessive antibiotic consumption may prevent sufficient access to these life-saving drugs among the most deprived in society because of the weak primary health care system. This makes antibiotic stewardship in the retail pharmacy sector a particular challenge. We conducted an analysis to examinate policies on antibiotic sales in retail pharmacies in China and how tensions between ‘excess’ and ‘access’ are managed. The analysis was guided by the Walt and Gilson health policy analysis triangle to systematically analyse policies based on the content of policies, contexts, governance processes, and actors. Nine research studies and 25 documents identified from national and international sources were extracted, grouped into categories, and examined within and across records and categories. As of 2020, eight key policies have been introduced in China that focus on two areas: dispending prescribed medicines or antimicrobials with a prescription and having a licensed pharmacist present in the retail pharmacies, with approaches having changed over time. Inappropriate sales of antibiotics are still common in retail pharmacies, which can be linked to the lack of consistency and enforcement of published policies, the profit-driven nature of retail pharmacies, and the displacement of the demand for antibiotics from clinical into less regulated settings.
Cynanchi atrati and Its Phenolic Constituent Sinapic Acid Target Regulator of Calcineurin 1 (RCAN1) to Control Skin Inflammation
Authors: Kim Seon Sook , Kim Nam Kyoung , Seo Su Ryeon
Published: January 2022

Atopic dermatitis (AD) is a common inflammatory skin disorder, and numerous pharmacological approaches are employed to reduce symptoms. Natural products of plant-derived materials have been accepted as complementary therapy for the treatment of a wide range of inflammatory diseases. Cynanchi atrati (CA) is an oriental medicinal herb used in the treatment of acute urinary infection, febrile diseases, and laryngopharyngitis. However, the role of CA root extract in skin inflammation such as AD has not been explored yet. In this study, we examined the possible effect of CA root extract on skin inflammation and evaluated the underlying signaling mechanism using in vitro and in vivo modeling systems. Raw264.7 macrophages were used for in vitro experiments, and an oxazolone-induced AD mouse model was used to evaluate in vivo effects. CA extract significantly inhibited the expression levels of lipopolysaccharide (LPS)-induced pro-inflammatory cytokines such as interleukin-6 (IL-6) and interleukin-1β (IL-1β) in RAW264.7 macrophages. The CA root extract mediated suppression of pro-inflammatory cytokine expression and was associated with the decreased nuclear factor kappa B (NF-κB) gene transcriptional activation. Moreover, CA root extract attenuated the in vivo expression of IL-6 and tumor necrosis factor-α (TNF-α) and ear swelling in the AD mouse models. We also observed that the inhibitory effect of CA root extract on skin inflammation was accompanied by the upregulation of calcineurin 1 (RCAN1) expression, which functions in the inflammatory pathways by suppressing NF-κB signaling. We consistently observed that the immunosuppressive effect of CA root extract in AD was significantly perturbed in the RCAN1 knockout mice. In addition, we isolated a phenolic acid compound, sinapic acid (SA), from the CA root extract and found that SA consistently exerted an immunosuppressive effect in RAW264.7 macrophages by inducing RCAN1 expression. Our results provide the first evidence that CA root extract and its phenolic acid constituent, SA, modulate NF-κB signaling pathways by inducing RCAN1 expression in the skin inflammation process. Thus, we suggest that CA root extract has a therapeutic value for the treatment of AD by targeting endogenous immune regulators.
Generation of Reactive Oxygen Species (ROS) by Harmful Algal Bloom (HAB)-Forming Phytoplankton and Their Potential Impact on Surrounding Living Organisms
Authors: Cho Kichul , Ueno Mikinori , Liang Yan , Kim Daekyung , Oda Tatsuya
Published: January 2022

Most marine phytoplankton with relatively high ROS generation rates are categorized as harmful algal bloom (HAB)-forming species, among which Chattonella genera is the highest ROS-producing phytoplankton. In this review, we examined marine microalgae with ROS-producing activities, with focus on Chattonella genera. Several studies suggest that Chattonella produces superoxide via the activities of an enzyme similar to NADPH oxidase located on glycocalyx, a cell surface structure, while hydrogen peroxide is generated inside the cell by different pathways. Additionally, hydroxyl radical has been detected in Chattonella cell suspension. By the physical stimulation, such as passing through between the gill lamellas of fish, the glycocalyx is easily discharged from the flagellate cells and attached on the gill surface, where ROS are continuously produced, which might cause gill tissue damage and fish death. Comparative studies using several strains of Chattonella showed that ROS production rate and ichthyotoxicity of Chattonella is well correlated. Furthermore, significant levels of ROS have been reported in other raphidophytes and dinoflagellates, such as Cochlodinium polykrikoides and Karenia mikimotoi. Chattonella is the most extensively studied phytoplankton in terms of ROS production and its biological functions. Therefore, this review examined the potential ecophysiological roles of extracellular ROS production by marine microalgae in aquatic environment.
A J-Protein OsDjC46 Interacts with ZFP36 to Participate in ABA-Mediated Antioxidant Defense in Rice
Authors: Huang Xingxiu , Huang Liping , Zhao Xixi , Jia Jing , Zhang Gang , Zhang Mengyao , Jiang Mingyi
Published: January 2022

ZFP36 has been shown to be involved in ABA-induced antioxidant defense and enhance rice tolerance to drought, salt stress and oxidative stress. Using ZFP36 as bait, a yeast two-hybrid system was used to obtain the interacting protein OsDjC46, which belongs to heat shock protein and usually exists in the form of molecular chaperone, was identified. Further Co-IP (co-immunoprecipitation), BiFC (bimolecular fluorescence complement) and GST (glutathione-S-transferase) pull-down experiments verified that ZFP36 interacted with OsDjC46 in vivo and in vitro. Heat shock protein has been shown to increase plant resistance to stresses, but whether OsDjC46 was a key factor in plant response to various stresses has not been reported. Here, various stimuli, such as abscisic acid (ABA), hydrogen peroxidase (H2O2), polyethylene (PEG) and sodium chloride (NaCl) markedly induced the expression of OsDjC46 in the seedlings. Overexpression of OsDjC46 in rice can enhance the tolerance to salinity and drought; in contrast, knockout of OsDjC46 rice plants was more sensitive to salt stress and drought. Further investigation revealed that OsDjC46 could participate in regulating the expression and activities of antioxidant of SOD and CAT under drought and salt stress. Taken together, these findings reveal a novel function of OsDjC46 in adjusting ABA-induced antioxidant defense.
Unraveling and Targeting Myocardial Regeneration Deficit in Diabetes
Authors: Molinaro Claudia , Salerno Luca , Marino Fabiola , Scalise Mariangela , Salerno Nadia , Pagano Loredana , De Angelis Antonella , Cianflone Eleonora , Torella Daniele , Urbanek Konrad
Published: January 2022

Cardiomyopathy is a common complication in diabetic patients. Ventricular dysfunction without coronary atherosclerosis and hypertension is driven by hyperglycemia, hyperinsulinemia and impaired insulin signaling. Cardiomyocyte death, hypertrophy, fibrosis, and cell signaling defects underlie cardiomyopathy. Notably, detrimental effects of the diabetic milieu are not limited to cardiomyocytes and vascular cells. The diabetic heart acquires a senescent phenotype and also suffers from altered cellular homeostasis and the insufficient replacement of dying cells. Chronic inflammation, oxidative stress, and metabolic dysregulation damage the population of endogenous cardiac stem cells, which contribute to myocardial cell turnover and repair after injury. Therefore, deficient myocardial repair and the progressive senescence and dysfunction of stem cells in the diabetic heart can represent potential therapeutic targets. While our knowledge of the effects of diabetes on stem cells is growing, several strategies to preserve, activate or restore cardiac stem cell compartments await to be tested in diabetic cardiomyopathy.
Disclosing the Antioxidant and Neuroprotective Activity of an Anthocyanin-Rich Extract from Sweet Cherry (Prunus avium L.) Using In Vitro and In Vivo Models
Authors: Filaferro Monica , Codeluppi Alessandro , Brighenti Virginia , Cimurri Francesca , González-Paramás Ana María , Santos-Buelga Celestino , Bertelli Davide , Pellati Federica , Vitale Giovanni
Published: January 2022

In this study, an autochthonous variety of sweet cherry (Prunus avium L.), namely “Moretta di Vignola”, was processed to prepare extracts rich in polyphenols, which were characterized by high-performance liquid chromatography (HPLC) separation coupled to UV/DAD and ESI-MSn analysis. Then, a sweet cherry anthocyanin-rich extract (ACE) was prepared, fully characterized and tested for its activity against Parkinson’s disease (PD) in cellular (BV2 microglia and SH-SY5Y neuroblastoma) and in Drosophila melanogaster rotenone (ROT)-induced model. The extract was also evaluated for its antioxidant activity on Caenorhabditis elegans by assessing nematode resistance to thermal stress. In both cell lines, ACE reduced ROT-induced cell death and it decreased, alone, cellular reactive oxygen species (ROS) content while reinstating control-like ROS values after ROT-induced ROS rise, albeit at different concentrations of both compounds. Moreover, ACE mitigated SH-SY5Y cell cytotoxicity in a non-contact co-culture assay with cell-free supernatants from ROT-treated BV-2 cells. ACE, at 50 µg/mL, ameliorated ROT (250 μM)-provoked spontaneous (24 h duration) and induced (after 3 and 7 days) locomotor activity impairment in D. melanogaster and it also increased survival and counteracted the decrease in fly lifespan registered after exposure to the ROT. Moreover, heads from flies treated with ACE showed a non-significant decrease in ROS levels, while those exposed to ROT markedly increased ROS levels if compared to controls. ACE + ROT significantly placed the ROS content to intermediate values between those of controls and ROT alone. Finally, ACE at 25 µg/mL produced a significant increase in the survival rate of nematodes submitted to thermal stress (35 °C, 6–8 h), at the 2nd and 9th day of adulthood. All in all, ACE from Moretta cherries can be an attractive candidate to formulate a nutraceutical product to be used for the prevention of oxidative stress-induced disorders and related neurodegenerative diseases.
Nrf2 Is Required for Optimal Alveolar-Macrophage-Mediated Apoptotic Neutrophil Clearance after Oxidant Injury
Authors: Reddy Narsa M. , Tamatam Chandra Mohan , Aparna Ankireddy , Reddy Sekhar P.
Published: January 2022

Recognition and clearance of apoptotic cells by phagocytes (also known as efferocytosis), primarily mediated by macrophages, are essential to terminate lung inflammatory responses and promote tissue repair after injury. The Nrf2 transcription factor is crucial for cytoprotection and host defense. Previously, we showed sustained neutrophilic lung inflammation in Nrf2-deficient (Nrf2−/−) mice after hyperoxia-induced lung injury in vivo, but the mechanisms underlying this abnormal phenotype remain unclear. To examine whether Nrf2 regulates apoptotic neutrophil clearance, we used the alveolar macrophages (AMФs) and bone-marrow-derived macrophages (BMDMФs) of wild-type (WT) and Nrf2−/− mice. We found that the efferocytic ability of AMФ was impaired in hyperoxia-exposed mice’s lungs, but the effect was more pronounced in Nrf2−/− mice. Importantly, AMФ-mediated efferocytosis remained impaired in Nrf2−/− mice recovering from injury but was restored to the basal state in the wild-type counterparts. Hyperoxia affected apoptotic neutrophil binding, not internalization, in both WT and Nrf2−/− BMDMФs, but the effect was more significant in the latter cells. Augmenting Nrf2 activity restored hyperoxia attenuated efferocytosis in WT, but not in Nrf2−/− macrophages. However, the loss of Nrf2 in neutrophils affected their uptake by WT macrophages. Collectively, these results demonstrate that Nrf2 is required for optimal macrophage-mediated efferocytosis and that activating Nrf2 may provide a physiological way to accelerate apoptotic cell clearance after oxidant injury.
Fermented Biomass of Arthrospira platensis as a Potential Food Ingredient
Authors: Jamnik Polona , Mahnič Nik , Mrak Aleksandra , Pogačnik Lea , Jeršek Barbara , Niccolai Alberto , Masten Rutar Jasmina , Ogrinc Nives , Dušak Larisa , Ferjančič Blaž , Korošec Mojca , Cerar Ana , Lazar Borut , Lovše Urša , Pungert Tjaša , Fabjan Primož , Poklar Ulrih Nataša
Published: January 2022

Lactic acid fermentation (LAF) is known to improve nutritional properties and functionality and to extend the shelf life of foods. We studied the LAF of Arthrospira platensis as the sole substrate using Lactobacillus plantarum as the starter culture. Fermented (FB) and non-fermented broth (NFB) were analysed by means of pH, lactic acid bacteria (LAB) count, lactic acid concentration, microbiological safety, and nutritional composition. Additionally, water and ethanol extracts were prepared on which total phenolic content, DPPH radical scavenging activity, and cellular antioxidant activity were determined. The maximum increase in LAB count and lactic acid concentration and drop in pH was observed in the first 24 h of fermentation. Total phenolic content and DPPH radical scavinging activity of ethanol extracts increased after fermentation compared with NFB. Ethanol extracts of FB have been shown as a potential source of antioxidants, which efficiently lowered oxidation level in the cells of yeast Saccharomyces cerevisiae, as well as the oxidative damage of lipids. Additionally, the level of non-protein nitrogen increased, indicating higher protein bioavailability, and fat content decreased in comparison with NFB. No presence of pathogenic bacteria and low pH indicate enhancement of FB microbiological stability. Therefore, inclusion of fermented A. platensis into food products could lead to added-value foods based on microalgae.
Evaluation and Validation of Estimated Sediment Yield and Transport Model Developed with Model Tree Technique
Authors: Kang Woochul , Lee Kyungsu , Jang Eun-kyung
Published: January 2022

This study evaluated the applicability of existing sediment yield and transport estimation models developed using data mining classification and prediction techniques and validated them. Field surveys were conducted by using an acoustic Doppler current profiler and laser in situ scattering and transmission at measuring points in the main stream of the Nakdong River located where the tributaries of the Geumho, Hwang, and Nam Rivers join. Surveys yielded estimations of water velocity, discharge, and suspended sediment concentrations were measured. In contrast with models based on the general watershed characteristics factors, some models based on hydraulic explanatory flow variables demonstrated an excellent predictability. This is because the selected submodels for validation, which provided excellent prediction results, were based on a large number of calibration data. It indicates that a sufficient number of reliable data is required in developing a sediment yield estimation model using data mining. For practical applications of data mining to extant sediment yield estimation models, comprehensive considerations are required, including the purpose and background of model development, and data range. Furthermore, the existing models should be periodically updated with the consideration of temporal and spatial lumping problems.
Normality in the Distribution of Revealed Comparative Advantage Index for International Trade and Economic Complexity
Authors: Liu Bin , Gao Jianbo
Published: January 2022

The Revealed Comparative Advantage (RCA) index is an important metric for evaluating competitiveness of a country in exporting certain commodity. While it is desirable to have a normally distributed RCA index, the opposite is often found in empirical studies, and efforts for developing alternative indices of the RCA index have not been very successful. This motivates us to ask a more fundamental question: what is the significance of a normally distributed RCA index? To answer this question, we have defined a quantity called the Deviation from Gaussianity (DfG) based on the KS test, which quantifies the deviation of the distribution of a country’s RCA index from normality. By systematically analyzing the distribution characteristics of RCA index for each country from 1991 to 2019, we find that DfG is strongly negatively correlated with the logarithm of GDP and the Economic Complexity Index (ECI). In particular, correlation between DfG and GDP is stronger than that between ECI and GDP since 2008. These results suggest that DfG may serve as a new excellent index to quantify the economic complexity and economic performance of a country.
Shannon (Information) Measures of Symmetry for 1D and 2D Shapes and Patterns
Authors: Bormashenko Edward , Legchenkova Irina , Frenkel Mark , Shvalb Nir , Shoval Shraga
Published: January 2022

In this paper, informational (Shannon) measures of symmetry are introduced and analyzed for patterns built of 1D and 2D shapes. The informational measure of symmetry Hsym(G) characterizes the averaged uncertainty in the presence of symmetry elements from group G in a given pattern, whereas the Shannon-like measure of symmetry Ωsym(G) quantifies the averaged uncertainty of the appearance of shapes possessing a total of n elements of symmetry belonging to group G in a given pattern. Hsym(G1)=Ωsym(G1)=0 for the patterns built of irregular, non-symmetric shapes, where G1 is the identity element of the symmetry group. Both informational measures of symmetry are intensive parameters of the pattern and do not depend on the number of shapes, their size, and the entire area of the pattern. They are also insensitive to the long-range order (translational symmetry) inherent for the pattern. Additionally, informational measures of symmetry of fractal patterns are addressed, the mixed patterns including curves and shapes are considered, the time evolution of Shannon measures of symmetry are examined, the close-packed and dispersed 2D patterns are analyzed, and an application of the suggested measures of symmetry for the analysis of the chemical reaction is demonstrated.
Aerodynamic Characteristics of Morphing Supercritical Airfoils for Aircraft with All-Stage High Performance
Authors: Cheng Yuwei , Zeng Jinyuan , Chen Qian , Liang Haizhao , Bai Peng
Published: January 2022

Morphing airfoil is a promising technology for future aircraft to realize all-stage high performance. In the present paper, a conceptual aircraft with morphing airfoil is proposed and the aerodynamic characteristics of three types of morphing airfoils (variable-camber airfoil, variable-chord airfoil, and the combination of both morphing styles) are numerically investigated. The baseline airfoil is RAE 2822 supercritical airfoil; the Reynolds-averaged Navier–Stokes method is adopted for numerical simulation of flow around airfoils, and the accuracy of the numerical simulation method is validated by comparing with experimental data. It is found that the variable-camber and -chord airfoil can not only improve the high lift characteristics at take-off stage, but also increase the lift-to-drag ratio at transonic cruise and low-speed task stages during which the required lift is continuously decreasing due to the consumption of fuel. These findings imply that aircraft with proper morphing airfoil can achieve all-stage high aerodynamic performance.