bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigation on Minimum Ventilation, Heating, and Energy Consumption of Pig Buildings in China during Winter
Authors: Qi Fei , Li Hao , Zhao Xuedong , Huang Jinjun , Shi Zhengxiang
Published: January 2023

Ventilation and heating can be necessary for pig production during winter in China. However, it is challenging to balance the ventilation rate and heat loss due to the ventilation. Therefore, it is essential to design the minimum ventilation and heating load properly in order to reduce energy loss. In this paper, a VBA (Visual Basic for Applications) model based on energy balance is established. Meteorological data, pig body masses, outdoor temperatures, feeding densities, and building envelope thermal insulance factors were involved in the model. A model pig house with a length and width of 110 m × 15 m was used to investigate the ventilation, heating time, load, and power consumption in different climate zones, i.e., Changchun, Beijing, Nanning, Wuhan, and Guiyang, representing five major climate regions in China. Based on the simulation results, the models of minimum ventilation and heating load were fitted. The results showed that there is a logarithmic relationship between the minimum ventilation volume and body mass, R2 = 0.9673. The R2 of heating load models for nursery pigs and fattening pigs were 0.966 and 0.963, respectively, considering the feeding area, the outside temperature, the body masses of the nursery and fattening pigs, and the thermal insulance factor of the enclosure. The heating requirements of commercial pig houses within the same building envelope followed the trend in Changchun > Beijing > Guiyang > Wuhan > Nanning. Increasing the building envelope’s thermal insulance factor or using precision heating could reduce the pig house’s power consumption. The analysis of the heating load and energy consumption of winter pig houses in various climate regions provided a reference for precise environmental control and the selection of building thermal insulance factors in China.
Experimental Research on a Needle Roller-Type Electrostatic Separation Device for Separating Unginned Cotton and Residual Films
Authors: Sun Yue , Hu Chunling , Li Yangyang , Chen Mingxiao , Zhang Ruoyu
Published: January 2023

In the mechanized harvesting of cotton in Northwest China, problems result from the presence of a residual film, and it is difficult to remove the residual film in the cotton processing stage. In this study, a needle roller electrostatic separation device was designed for use with unginned cotton residual films, and a series of experiments was carried out. Through force analyses and charge testing of the seed cotton and the residual film, a theoretical basis was provided for separating them. Taking the residual film separation rate as the response index, the optimal parameter combination was determined with a response surface analysis of three factors and three levels, and a mathematical model was established. The test results showed that the optimal parameter set included a 25.3° corona electrode angle, a 172.3 mm corona electrode distance, and a 5.25 W discharge power. After optimization of the parameters for separation, the average residual film separation rate was 91.7%. This research provides data and technical support for the design of the machinery and the selection of the parameters used in treating machine-picked cotton and separating the residual film.
Serum Cytokine Reactions during Pregnancy in Healthy Mares
Authors: Figarska Aleksandra , Domino Małgorzata , Maśko Małgorzata , Witkowska-Piłaszewicz Olga
Published: January 2023

The aim of the research was to determine the differences in IL-1ß, IL-2, IL-4, IL-10, IL-17, INF-γ, TNF-α and IL-1ra blood concentrations in pregnant and non-pregnant healthy mares. The mares were divided into two groups: pregnant mares (n = 18; age 6.11 ± 2.25 years); non-pregnant mares (n = 6; age 5.67 ± 1.75 years). Blood samples were collected from all mares (n = 24) three times every month, and then the pregnant group was divided into three subgroups based on the age of the pregnancy (1st group (n = 6)—6th, 7th, 8th; 2nd (n = 6)—7th, 8th, and 9th; 3rd (n = 6)—8th, 9th, 10th month of pregnancy). The concentrations of IL-1ß, IL-2, IL-4, IL-10, IL-17, INF-γ, and TNF-α were higher in the pregnant than in non-pregnant group, whereas only the concentration of IL-1ra was lower in the pregnant than in the non-pregnant groups. Serum concentrations of proinflammatory cytokines such as IL-1 β, IL-2, and IFN-γ in pregnant mares were greater than in non-pregnant mares at the start of the trimester, whereas, IL-4, IL-17 and TNF-α started to rise in the latter months of the third trimester. IL-10 and IL-1ra concentrations started to decrease after the 6th month of pregnancy. In non-pregnant mares cytokine levels were stable during the whole study. In mares, the change in the ratio between Th1 (IL-1, IL-2, INF-γ, TNF-α) and Th2 (e.g., IL-4, IL-10, IL-13) cytokines occurs during pregnancy. The cytokine profile may be useful in the future for monitoring healthy pregnancies; however, more research is needed especially for miscarriage in mares.
Characterization of Lupin Cultivars Based on Phenotypical, Molecular and Metabolomic Analyses
Authors: Mavromatis Athanasios , Nianiou-Obeidat Irini , Polidoros Alexios , Parissi Zoi , Tani Eleni , Irakli Maria , Aliferis Konstantinos A. , Zafeiriou Ioannis , Mylona Photini V. , Sarri Efi , Papadopoulou Evgenia-Anna , Tagiakas Rafail , Kougiteas Leonidas , Kostoula Stavroula , Abraham Eleni M.
Published: January 2023

Lupins are an important source of protein that could replace soybeans in the diet of ruminants and monogastrics, without reducing their performance. Lupinus albus (L. albus) is the main species of the genus Lupinus that is cultivated in the Mediterranean region. The aim of the present research was to study commercial cultivars and advanced breeding lines of L. albus by using phenotypical, molecular and biochemical data, in order to be used in breeding projects. Seven commercial cultivars (Estoril, Fas Sweet, Multitalia, Magnus, Orus, Ulysse Sulimo and Figaro) and three advanced lines from the company AGROLAND (LKML, LKAP and LKAU) were used. Eleven morphological traits were described using UPOV Guidelines (International Union for the Protection of New Varieties of Plants). Additionally, agronomical traits and yield components were measured. Regarding the nutritional value, grain samples were analyzed for N and the crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), total alkaloids (TA), total phenolic content (TP), total tannins content (TT) and condensed tannins (CT) were calculated. Genetic diversity among genetic materials was assessed by SSRs molecular markers. The metabolomic analysis for four selected cultivars (Figaro, Magnus, Multitalia and Sulimo) was performed on the seeds with the GC/EI/MS technique. According to the results, the advanced lines were most productive but also with higher content of total alkaloids than the commercial cultivars. The only exception was the cultivar Multitalia that was characterized by a high content of alkaloids. Based on the SSRs, the cultivars Magnus, Orus and Estoril were grouped together while the breeding lines LKAP, LICML and LKAU were grouped with Multitalia. Regarding the metabolomic profile, the cultivars Multitalia and Magnus were together, while Sulimo was grouped with Figaro. Finally, the content of several beneficial metabolites for human and animal nutrition was significantly increased in Sulimo and Figaro, compared to Magnus and Multitalia. Both commercial varieties and lines have characteristics that can be exploited and used in breeding programs.
Drought and Waterlogging Status and Dominant Meteorological Factors Affecting Maize (Zea mays L.) in Different Growth and Development Stages in Northeast China
Authors: Wang Xiaowei , Li Xiaoyu , Gu Jiatong , Shi Wenqi , Zhao Haigen , Sun Chen , You Songcai
Published: January 2023

Drought and floods affect the growth and yield of maize, affecting food security. Therefore, it is crucial to assess maize’s drought and waterlogging status in various growth stages. We used phenological and daily meteorological data and spatial analysis to identify the drought and waterlogging conditions of spring maize in Northeast China in eight growth stages. We calculated the crop water surplus/deficit index and used the national standard for maize drought and waterlogging. The results indicate a significant decreasing trend of effective precipitation in Northeast China. The maize’s water requirements changed during the growing period. The ranking of the daily water requirements of maize from high to low in the different growth stages was the flowering stage to the silking stage (6.9 mm/d), the tasseling stage to the flowering stage (6.1 mm/d), the jointing stage to the tasseling stage (4.9 mm/d), the seven-leaf stage to the jointing stage (3.4 mm/d), the silking stage to the harvesting stage (2.0 mm/d), the emergence stage to the three-leaf stage (1.4 mm/d), the three-leaf stage to the seven-leaf stage (1.3 mm/d), and the sowing stage to the emergence stage (1.2 mm/d). Drought occurred primarily in the early growth and development stage, and the most severe drought conditions were observed in the sowing to emergence stages and the emergence to the three-leaf stages in most areas in Northeast China. Waterlogging occurred predominantly in the flowering to the silking stages and the silking to the maturity stages in southeast Liaoning and parts of Jilin. Inner Mongolia had the lowest soil moisture conditions and was unsuitable for maize growth, followed by Heilongjiang, Jilin, and Liaoning. The dominant meteorological factors affecting the drought and waterlogging status of maize in different growth stages were precipitation and wind speed, followed by the minimum temperature, relative humidity, sunshine hours, and maximum temperature. The average temperature did not influence the drought and waterlogging status. The results provide a basis for selecting drought-resistant varieties and preventing waterlogging.
Study of the Mexican Cocoa Market: An Analysis of Its Competitiveness (2010–2021)
Authors: Duana-Ávila Danae , Hernández-Gracía Tirso Javier , Martínez-Muñoz Enrique , García-Velázquez Ma del Rosario , Román-Gutiérrez Alma Delia
Published: January 2023

Cocoa is one of the main products consumed worldwide that is similar to coffee; a primary difference between coffee and cocoa is that cocoa is produced in developing countries and is consumed mainly in industrialized countries. Mexico was the country that made cocoa known to the world. The objective of the study was to analyze the competitiveness at the macroeconomic level of the Mexican cocoa product in the world during the period 2010–2021. A macroeconomic analysis was carried out using six indices: Balassa index, Vollrath Index, Additive Revealed Competitive Advantage Ratio, Trade Openness Index, Export and Import Index, and Self-Sufficiency Index. The industrialization of cocoa is the denaturation of the product, which is launched onto the market with little or no nutritional content, due to the alteration of the raw material with formulas based on vegetable fats, artificial colors, and flavors. For example, a commercial chocolate bar can have only 25–35% cocoa mass, semisweet chocolate bars can vary from 45 to 99% cocoa, while white chocolate bars only contain cocoa butter, milk, and sugar. These facts result in a decrease in the competitiveness of the product in the international market, in addition to the effects produced on its profitability for the producer—an increase in the cost of raw materials and a drop in real profits. These factors generate dependency on the international market for the production.
Modeling the Effects of Rice-Vegetable Cropping System Conversion and Fertilization on GHG Emissions Using the DNDC Model
Authors: Sun Xiaolu , Yang Xiaohui , Hou Jinjin , Wang Bisheng , Fang Quanxiao
Published: January 2023

The cropping system conversion, from rice to vegetable, showed various influences on the greenhouse gases (GHG) emission with conversion time and fertilizer/irrigation management. In this study, we evaluated the DeNitrification-DeComposition (DNDC) model for predicting carbon dioxide (CO2), methane (CH4), nitrous oxide (N2O) emissions and crop yields as rice converted to vegetable cropping system under conventional or no fertilization from 2012 to 2014. Then, we quantified the long-term (40 years) impacts of rice-vegetable cropping system conversions and fertilization levels (0, 50, 100 and 150% conventional fertilization rate) on GHGs emissions and global warming potentials (GWP) using the calibrated model. The DNDC model-simulated daily GHG emission dynamics were generally consistent with the measured data and showed good predictions of the seasonal CH4 emissions (coefficient of determination (R2) = 0.96), CO2 emissions (R2 = 0.75), N2O emissions (R2 = 0.75) and crop yields (R2 = 0.89) in response to the different cropping systems and fertilization levels across the two years. The overall model performance was better for rice than for vegetable cropping systems. Both simulated and measured two-year data showed higher CH4 and CO2 emissions and lower N2O emissions for rice than for vegetable cropping systems and showed positive responses of the CO2 and N2O emissions to fertilizations. The lowest GWP for vegetable without fertilization and highest the GWP for rice with fertilization were obtained. These results were consistent with the long-term simulation results. In contrast to the two-year experimental data, the simulated long-term CH4 emissions increased with fertilization for the rice-dominant cropping systems. The reasonable cropping systems and fertilization levels were recommended for the region.
Potassium Phosphite Induces Tolerance to Water Deficit Combined with High Irradiance in Soybean Plants
Authors: Batista Priscila Ferreira , da Costa Alan Carlos , da Silva Adinan Alves , Almeida Gabriel Martins , Rodrigues Maria Fernanda Marques , Santos Emily Carolina Duarte , Rodrigues Arthur Almeida , Müller Caroline
Published: January 2023

Changes in plant metabolism due to water deficit combined with other stresses, such as high irradiance and high temperatures, cause damage to the physiology and development of crops, which can lead to significant yield losses. The aim of this study was to determine the potential of potassium phosphite (PP) to induce tolerance to water deficit combined with high irradiance in soybean plants. The experiment was carried out in an acclimatized growth chamber. Soybean plants, upon reaching the R1 developmental stage, received the following treatments: PP application (0 L ha−1–control; 0.6 L ha−1 PP; and 1.2 L ha−1 PP), two levels of PAR irradiance (650 µmol m−2 s−1–control; and 1500 µmol m−2 s−1–high irradiance (HI)), and three water availability levels (90% of field capacity (FC), and water deficit at 40% FC and 50% FC). The treatments were maintained for 12 days. The PP increased the photosynthetic rate of plants submitted to a dosage of 1.2 L ha−1 and stresses of 50% FC + HI. PP also decreased the intensity of lipid peroxidation, and rate of electrolyte leakage, which suggests stability of cell membranes. These responses may have occurred due to the activation of the antioxidant enzymes superoxide dismutase and peroxidase. Furthermore, the application of PP increased the proline concentrations, suggesting osmotic adjustment in response to stress. These results provide the first record of PP-induced tolerance in plants under combined water and HI stresses. PP proves to be a potential alternative method to reduce the harmful effects caused by the combined stresses of water deficit and high irradiance in soybean.
Building Resilient Crop Production Systems for Drought-Prone Areas—A Case for Bambara Groundnut (Vigna subterranea L. Verdc) and Groundnut (Arachis hypogaea L.)
Authors: Kundy Aloyce Callist , Mayes Sean , Msanya Balthazar , Ndakidemi Patrick , Massawe Festo
Published: January 2023

Drought is a major crop production constraint worldwide. Some legume crops are known for their ability to resist water deficit stress. This study evaluated the responses of bambara groundnut (Vigna subterranea (L.) Verdc) and groundnut (Arachis hypogaea (L.) to soil water deficit stress. The experiment was set as a split-plot randomized complete block design. Three bambara groundnut landraces: viz DodR, NALBAM 4 and S19-3, and one groundnut variety, MNANJE, were assigned to subplots with three water regimes assigned to main plots (regime one: irrigated throughout the growing period, regime two: water deficit stress was imposed at the start of flowering to the end of first flush flowering, regime three: water was withheld during the pod development). Water deficit stress increased proline content by 123% in stressed plots. The highest (174%) and lowest (89%) proline increases were evident in the genotypes MNANJE and NALBAM 4, respectively. Water deficit decreased stomatal conductance, transpiration rate and photosynthetic rate, with MNANJE and S19-3 showing the smallest percentage decrease in most of the traits. This suggests that the two genotypes are drought resistant. The variations observed among landraces could be exploited to breed resilient varieties for cultivation in drought-prone areas, ultimately improving food security.
A Simulation Study on Optimization of Sowing Time of Maize (Zea mays L.) for Maximization of Growth and Yield in the Present Context of Climate Change under the North China Plain
Authors: Wu Yixuan , Zhou Guangsheng , Song Yanling , Ren Sanxue , Geng Jinjian , Zhao Huarong , Song Xingyang
Published: January 2023

Adjusting the sowing dates of crops is an effective measure for adapting them to climate change, but very few studies have explained how the optimum sowing dates can be determined. In this study, we used the sowing date field data from 2018 to 2021 from Hebei Gucheng Agricultural Meteorology National Observation and Research Station to analyze the effects of the sowing date on growth, development, and yield of maize, and to quantify the impact of light-temperature potential productivity on different stages of the yield formation. The results showed that delayed sowing decreased the vegetative growth period (VGP) and increased the reproductive growth period (RGP) of maize. The light-temperature potential productivity of the whole growth (WG) period had an exponential relationship with the theoretical yield. At least 14,614.95 kg ha−1 of light-temperature potential productivity was needed to produce grain yield. The maximum theoretical yield was approximately 18,052.56 kg ha−1, as indicated by the curve simulation results. The influence of light-temperature potential productivity on theoretical yield was as follows: VGP > RGP > vegetative and reproductive period (VRP). Accordingly, a method for determining the sowing time window based on VGP was established, and the optimal sowing dates were estimated for 1995–2021 and the SSP2-4.5 scenario in CMIP6 in the middle of this century (2030–2060). The simulation results showed that the optimum sowing date of maize “Lianyu 1” at the study site was 20–25 May in 1995–2021. In the middle of this century, the optimal sowing time of maize was ahead of schedule and the suitable sowing window was increased slightly. We conclude that advancing the sowing date of maize is a practical strategy for enhancing yield in the context of climate warming, and this strategy will provide a meaningful reference for scientific optimization of sowing dates to adapt maize to climate change.
Design and Test of Obstacle Detection and Harvester Pre-Collision System Based on 2D Lidar
Authors: Shang Yehua , Wang Hao , Qin Wuchang , Wang Qian , Liu Huaiyu , Yin Yanxin , Song Zhenghe , Meng Zhijun
Published: January 2023

Aiming at the need to prevent agricultural machinery from colliding with obstacles in the operation of unmanned agricultural machinery, an obstacle detection algorithm using 2D lidar was proposed, and a pre-collision system was designed using this algorithm, which was tested on a harvester. The method uses the differences between lidar data frames to calculate the collision times between the farm machinery and the obstacles. The algorithm consists of the following steps: pre-processing to determine the region of interest, median filtering, and DBSCAN (density-based spatial clustering of applications with noise) to identify the obstacle and calculate of the collision time according to the 6σ principle. Based on this algorithm, a pre-collision system was developed and integrated with agricultural machinery navigation software. The harvester was refitted electronically, and the system was tested on a harvester. The results showed that the system had an average accuracy rate of 96.67% and an average recall rate of 97.14% for being able to stop safely for obstacles in the area of interest, with a summed average of 97% for both the accuracy and recall rates. The system can be used for an emergency stop when encountering obstacles in the automatic driving of agricultural machinery and provides a basis for the unmanned driving of agricultural machinery in more complex scenarios.
Cultural Characterization and Antagonistic Activity of Cladobotryum virescens against Some Phytopathogenic Fungi and Oomycetes
Authors: Hernández Giselle , Ramos Beatriz , Sultani Haider , Ortiz Yarelis , Spengler Iraida , Castañeda Rafael , Rivera Daniel , Arnold Norbert , Westermann Bernhard , Mirabal Yaneris
Published: January 2023

In this study, the characteristic growth of Cladobotryum virescens on nine culture media was analyzed. The growing behavior of this fungus was dependent on the culture medium. In vitro analysis showed that oat agar was better than other media tested with the highest conidia production. The antifungal activity against Fusarium chlamydosporum and Alternaria brassicicola was evaluated by the Dual Culture method. C. virescens displayed high activity against both pathogens acting through antibiosis and mycoparasitism. This effect was increased by a higher competitiveness of the strain for the substrate. Furthermore, the crude ethyl acetate extract of the culture broth was tested in vitro against Botrytis cinerea and Septoria tritici, as well as the hemibiotrophic oomycete Phytophthora infestans using a microtiter plate assay at different concentrations. The extract showed excellent inhibition even below 5 ppm. According to these results, we concluded that C. virescens can be considered as a potential biological control agent in agriculture. To the best of our knowledge, this is the first study to investigate C. virescens as a biocontrol agent for different diseases caused by five relevant pathogens that affect cereals and vegetables.
Nano-Organic Coatings Improve Early Vigor of Brassica napus L. Seeds in Water Deficit
Authors: Abrahimi Farkhondeh , Taghvaei Mansour , Mastinu Andrea
Published: January 2023

Low emergence and vigor of canola seeds are common problems encountered by canola growers. Seed coating is a good way to solve this problem. The objective of this study was to find the best combination of nano-organic to be used as a coating for seeds for strengthening the absorption of water and emergence seed of low vigor canola. The treatments in the first experiment included six levels of organic coatings: 20% vermicompost with 80% bentonite, 30% vermicompost with 70% bentonite, 40% vermicompost with 60% bentonite, 40% residues of canola with 60% bentonite, 30% residues of canola with 70% bentonite, and 20% resides of canola with 80% bentonite. The results indicated that 20% residues of canola and 20% vermicompost had the highest moisture under the saturation conditions and kept moisture for a longer time. Subsequently, the factors of the second experiment included three levels of water stress (100, 75, and 50% field capacity (FC)), three levels of seed coating (control, vermicompost, and canola residues), and three levels of titanium dioxide nanoparticles (0, 0.5, and 1 mM). The results showed that nano-organic coating improved emergence percentage and seedling traits while increasing superoxide dismutase, peroxidase, and catalase activities and decreasing electrolyte leakage. The highest ratios for emergence percentage (74.67), seedling vigor index (264.58), and leaf area (418) were obtained at the highest level of nano-titanium (1 mM). The highest rates of superoxide dismutase (53.44), peroxidase (30.68), catalase (5.35), chlorophyll content (51.05), and lowest electrolyte leakage (42.39) were observed in the highest level of nano-titanium (1 mM). Based on the results, we suggest the use of rapeseed residues with nano titanium oxide for rapeseed coating. The use of nanoparticles in current seed coatings is useful for reducing stresses in the germination and emergence stages.
Soil Compaction and Maize Root Distribution under Subsoiling Tillage in a Wheat–Maize Double Cropping System
Authors: Sun Qing , Sun Wu , Zhao Zixuan , Jiang Wen , Zhang Peiyu , Sun Xuefang , Xue Qingwu
Published: January 2023

Huang-Huai-Hai Plain is the most important region for grain production in China. In this area, long-term rotary tillage in winter wheat and no tillage in summer maize have significantly increased soil bulk density, which impede maize root growth and reduce the grain yield. Subsoiling tillage is an effective practice to improve soil properties and crop growth. The objective of this study was to investigate the integrated effects of subsoiling tillage in both winter wheat and summer maize seasons on soil bulk density, maize root growth and spatial distribution. A two-year field experiment was conducted in winter wheat–summer maize rotation system. Tillage treatments included rotary tillage (RT) and subsoiling tillage (ST) in wheat season, and no tillage (NT), inter–row subsoiling tillage (STIR), and on–row subsoiling tillage (STOR) in maize season. It was found that in the second year, i.e., in 2018, ST decreased soil bulk density by 3.87% and increased porosity by 5.86% at 30–40 cm soil depth at maize maturity. Meanwhile, maize root length density at 40–50 cm depth increased by 30.00% and grain yield increased by 4.70% under ST. In maize season tillage treatments, STOR decreased soil bulk density by 4.52% and increased soil porosity by 6.96% at 20–30 cm soil depth. Compared with NT, the STOR significantly increased maize root length density at 20–30 cm soil depth by 78.45%, and increased root length density in a horizontal area 0–10 cm for both years, with a significant increase of 58.89% in 2018. Therefore, this study demonstrated that in the Huang-Huai-Hai Plain, which has a tidal soil type, subsoiling tillage in winter wheat season and on–row subsoiling tillage in maize season can loosen the soil and improve vertical extension of maize root system in the soil.
Anaerobic Digestion Reduces Seed Germination and Viability of Six Plant Species from the Upper Nile Valley, Egypt
Authors: Abbas Ahmed M. , Abdelazeem Mohamed , Novak Stephen J.
Published: January 2023

Anaerobic digestion (AD) involves the breakdown of a substrate by mixed microbial communities in the absence of free oxygen. This process has many benefits leading to the production of bioenergy (biogas) and fertilizers (bio-fertilizers). Unfortunately, the bio-fertilizer made using AD may be contaminated with weed seeds and may have the potential of infesting the fields to which it is applied. Thus, the goal of this study is to determine the effects of AD on seed germination and viability of two undesirable crop plants (Triticum aestivum and Sorghum bicolor) and four weed species of reclaimed agricultural land near Qena, Egypt (Schouwia purpurea, Polygonum equisetiforme, Amaranthus viridis, and Lotus arabicus). Vegetative biomass of T. aestivum was used as the substrate, and cattle rumen fluid was used as the inoculum. The seeds of the six plants were placed in culture bottles and subjected to AD at 37 ± 2 °C for up to 16 days. Time to first germination (the time required for the first seed to germinate) and the percentage of seeds that germinated varied among the six species. The percentage germination of all six species decreased with increasing duration of AD, with no seeds germinating at 12 or 16 days of incubation. Seeds of T. aestivum and S. bicolor experienced a rapid and significant decrease in the percentage of viable seeds and an increase in the percentage of non-viable seeds. In contrast, even though they did not germinate, some seeds of the other four weed species remained viable, even after 16 days of incubation. Approximately 32% of S. purpurea seeds remained viable at 16 days of incubation. Overall, our results suggested that the application of anaerobic digestate in the reclaimed agricultural lands near Qena, Egypt, is not likely to exacerbate weed infestations in these fields.
Assessment of the Cadmium and Copper Phytoremediation Potential of the Lobularia maritima Thioredoxin 2 Gene Using Genetically Engineered Tobacco
Authors: Ben Saad Rania , Ben Romdhane Walid , Baazaoui Narjes , Bouteraa Mohamed Taieb , Ben Hsouna Anis , Mishra Avinash , Ćavar Zeljković Sanja
Published: January 2023

Rapid global modernization, urbanization, and industrialization have accelerated the release of heavy metals, causing soil pollution. These highly noxious environmental pollutants induce oxidative stress in plants via stimulation of the production of reactive oxygen species (ROS). Thioredoxin (Trxs) is a highly conserved disulfide reductase that plays a crucial role in intracellular redox homeostasis in both eukaryotes and prokaryotes. Herein, the presence of heavy metals highly upregulated LmTrxh2 transcription in Lobularia maritima seedlings and its overexpression-conferred tolerance to Cd, Cu, Mn, and Zn in Saccharomyces cerevisiae. In addition, LmTrxh2-overexpressing tobacco plants had higher seedling survival rates than non-transgenic plants (NT), with enhanced root length and biomass production and reduced ROS accumulation, following Cd and Cu stress. These plants also accumulated more Cd, Cu, and Mn than the NT plants. Moreover, LmTrxh2 overexpression stimulated the transcription of genes encoding metallothioneins (Met-1, Met-2, Met-3, and Met-4), a copper transport protein, a Snakin/GASA protein (Snakin-2), and ROS-scavenging enzymes (SOD, APX1, and CAT), which might contribute to heavy metal tolerance in tobacco plants. These results suggest that LmTrxh2 overexpression helps to improve heavy metal tolerance by stimulating antioxidant capacities and the expression of several stress-responsive genes in plants.
Seed-Primed and Foliar Oxozinc Nanofiber Application Increased Wheat Production and Zn Biofortification in Calcareous-Alkaline Soil
Authors: Ahmad Wiqar , Zou Zhiyou , Awais Muhammad , Munsif Fazal , Khan Aziz , Nepal Jaya , Ahmad Masood , Akbar Sultan , Ahmad Ijaz , Khan Muhammad Shahid , Qamar Zahid , Khan Haroon
Published: January 2023

Low Zinc (Zn) availability in alkaline calcareous soil is one of the major causes of low cereal yield and quality. Conventional application of Zn sulfate (ZnSO4) fertilizer through soil application attains minimal Zn efficiency as it is readily fixed in such soils. Oxozinc nanofiber (ZnONF) was evaluated for wheat Zn biofortification using different application methods to tackle this issue. Pots in triplicate (each with 7 kg soil) were arranged in a completely randomized design with a control treatment without Zn application. The conventional ZnSO4 fertilizer recommended dose (5.5 µg Zn kg−1 of soil) was used for comparison and applied through soil addition, foliar spray, and seed priming, while the ZnONF was applied through foliar spray, seed coating, and seed priming (@ 0.5 kg ha−1) either alone or in combination with ½ZnSO4 applied to the soil. The application of ZnONF significantly improved wheat plant growth as evidenced by increased plant height (14.5%), spikelets per spike (13.7%), and Zn use efficacy (611%) regardless of application methods as compared to control. The highest Zn uptake efficiency (34%) for nanofibers was obtained for theseed primed, followed by seed coating (23%) and foiar application (7%), respectively. Moreover, at the combined ZnONF and ½ZnSO4 application, further improvements for spike length, number of spikelets spike−1, grain, leaf, root, and stem Zn concentrations, as well as their respective Zn contents, were noted. These results elucidated that Zn nutrition with ZnONF was either at par with or higher than the conventional ZnSO4 fertilizer application despite significantly reduced ZnONF quantity, irrespective of the application method used. Additionally, the combined ZnONF and ½ZnSO4 (foliar spray, seed coating, or seed priming) maximized the crop Zn accumulation, wherein the ½ZnSO4 + ZnONF through foliar application exceeded grain Zn biofortification. Thus, various Oxozinc nanofibers application modes may be recommended for wheat biofortification either separately or in combination with ZnSO4 in Zn deficient calcareous soils for improved Zn nourishment.
Physiological and Enzymatic Evaluation of Selected Genotypes of Stevia rebaudiana Bertoni
Authors: Rodriguez-Paez Luis Alfonso , Jimenez-Ramirez Ana Melisa , Pompelli Marcelo F. , Pineda-Rodriguez Yirlis Yadeth , Jarma-Orozco Alfredo , de Dios Jaraba-Navas Juán , Aramendiz-Tatis Hermes , Combatt-Caballero Enrique , Oloriz-Ortega Maria Ileana , Rodríguez Novisel Veitía
Published: January 2023

The Germplasm Bank of Stevia rebaudiana segregants of the University of Córdoba is a strategy for the use of genetic variability and efficient crop yield. There are genotypes with important characteristics such as: high tolerance to salt stress and climatic change (high CO2 in the Earth surface), late flowering, erect architecture, and high contents of steviol glycosides. However, there is a lack of in-depth studies of morphophysiological and biochemical indicators such as gas exchange, chlorophyll a fluorescence, chloroplast pigments, and antioxidant systems, which allow us to develop early selection tools for elite genotypes. The genotypes (L020, Morita II, and L102) were found to have elite characteristics such as high efficiency in water use, excellent biomass production, and a more robust antioxidant system than the genotypes (L057 and L082). The L020 genotype presented the highest content of stevioside and rebaudioside A, followed by the Morita II genotype. We found a close correlation between the electron transport rate and the mechanisms that increase photosystem complexes. In this sense, non-photochemical cooling modulated by the release of heat by the leaves is a fact that is confirmed by the greater activity of the xanthine pool to protect the photochemical complexes in S. rebaudiana.
Effect of Thermal Activation Temperature of Nanoscale Phosphate and Silicate on the Morphological Transformation Characteristics of Cd in Heavy Metal Contaminated Soils
Authors: Wang Xiuli , Zou Hongtao
Published: January 2023

Purpose. The problem of heavy metal Cd pollution in the soil is still very serious. The widely used treatment measure is in situ passivation chemical remediation technology. Some studies have shown that the single application of phosphate or silicate can control soil cadmium pollution, but few studies have been conducted on the effect of the thermal activation temperature of nano phosphate and silicate-combined application on the transformation of Cd form in the soil. Materials and methods. Cadmium-contaminated soil was indoor-simulated, after which the 2.0% soil-weight dose of potassium dihydrogen phosphate was mixed with thermal activation nano serpentine or nano zeolite (potassium dihydrogen phosphate: thermal activation nano serpentine or nano zeolite = 1:2) at different temperatures (0, 350, 550, 700, 850 °C) and compared with the simulated cadmium-contaminated soil without adding a mixture of phosphate and silicate; cadmium content in soil was determined by Tessier five-step continuous extraction method after incubating for 0, 7, 14, 28, and 56 d in jars in the constant-temperature box. Results and discussion. Thermal activation temperature of nano phosphate and silicate could reduce the amount of exchangeable Cd content in the soil to a different degree; the content of Cd in carbonate-bound form, Fe–Mn–oxide-bound form, organically-bound form, and residual form increased. The order of effect of nanoscale potassium dihydrogen phosphate and serpentine or zeolite thermal activation temperature on remediation of Cd contaminated soil is nPS700-2.0 > nPS550-2.0 > nPS850-2.0 > nPS350-2.0 > nPS0-2.0, nPF700-2.0 > nPF550-2.0 > nPF850-2.0 > nPF350-2.0 > nPF0-2.0 (n, P, S, and F represent nanometer, KH2PO4, serpentine, and zeolite, respectively; 0, 350, 550, 700, and 850 represent different activation temperatures T); 700 °C treatments performed better than other thermal activation temperature treatments, and nPS700-2.0 was better than nPF700-2.0. Conclusion. Thermal activation temperature of nano phosphate and silicate-combined application can stabilize heavy metal Cd to some extent and promote the transition of Cd from a bioavailable state to a biounavailable state. The results showed that the combined application of thermal activation temperature of nano phosphate and silicate has a certain potential to control soil cadmium pollution.
Principles and Practices in Fruit Tree Production and Postharvest Management
Authors: Ziogas Vasileios
Published: January 2023

In the near future, the world’s population will face serious challenges due to continuous growth, global climate change, and limited natural resources [...]
Based on FCN and DenseNet Framework for the Research of Rice Pest Identification Methods
Authors: Gong He , Liu Tonghe , Luo Tianye , Guo Jie , Feng Ruilong , Li Ji , Ma Xiaodan , Mu Ye , Hu Tianli , Sun Yu , Li Shijun , Wang Qinglan , Guo Ying
Published: January 2023

One of the most important food crops is rice. For this reason, the accurate identification of rice pests is a critical foundation for rice pest control. In this study, we propose an algorithm for automatic rice pest identification and classification based on fully convolutional networks (FCNs) and select 10 rice pests for experiments. First, we introduce a new encoder–decoder in the FCN and a series of sub-networks connected by jump paths that combine long jumps and shortcut connections for accurate and fine-grained insect boundary detection. Secondly, the network also integrates a conditional random field (CRF) module for insect contour refinement and boundary localization, and finally, a novel DenseNet framework that introduces an attention mechanism (ECA) is proposed to focus on extracting insect edge features for effective rice pest classification. The proposed model was tested on the data set collected in this paper, and the final recognition accuracy was 98.28%. Compared with the other four models in the paper, the proposed model in this paper is more accurate, faster, and has good robustness; meanwhile, it can be demonstrated from our results that effective segmentation of insect images before classification can improve the detection performance of deep-learning-based classification systems.
Fertility Control for Wildlife: A European Perspective
Authors: Massei Giovanna
Published: January 2023

Trends of human population growth and landscape development in Europe show that wildlife impacts are escalating. Lethal methods, traditionally employed to mitigate these impacts, are often ineffective, environmentally hazardous and face increasing public opposition. Fertility control is advocated as a humane tool to mitigate these impacts. This review describes mammalian and avian wildlife contraceptives’ effect on reproduction of individuals and populations, delivery methods, potential costs and feasibility of using fertility control in European contexts. These contexts include small, isolated wildlife populations and situations in which lethal control is either illegal or socially unacceptable, such as urban settings, national parks and areas where rewilding occurs. The review highlights knowledge gaps, such as impact of fertility control on recruitment, social and spatial behaviour and on target and non-target species, provides a decision framework to assist decisions about the potential use of wildlife fertility control, and suggests eight reasons for Europe to invest in this area. Although developing and registering contraceptives in Europe will have substantial costs, these are relatively small when compared to wildlife’s economic and environmental impact. Developing safe and effective contraceptives will be essential if European countries want to meet public demand for methods to promote human–wildlife coexistence.
Monitoring Green Sea Turtles in the San Gabriel River of Southern California
Authors: Massey Lynn M. , Penna Shannon , Zahn Eric , Lawson Dan , Davis Cassandra M.
Published: January 2023

Effective conservation of endangered species relies on the characterization of habitat use and tracking of long-term population trends, which can be especially challenging for marine species that migrate long distances and utilize a diversity of habitats throughout their lives. Since 2012, citizen science volunteers at the Aquarium of the Pacific in Long Beach, California, have been monitoring an urban population of East Pacific green sea turtles (Chelonia mydas) that resides near the mouth of the San Gabriel River (SGR) in Southern California, USA, in order to gain insights about how the population uses this area. Here, we collate and analyze nine years of citizen science data, including observed sightings collected across 10 observation stations. Our results confirm that green sea turtles are frequently present around warm water effluent from power plants, similar to research results reported for other locations in the eastern Pacific Ocean. Importantly, observational data also show notable green sea turtle activity around the outfalls for a small wetland habitat bordering the SGR, highlighting the importance of wetland ecosystems as a key habitat and foraging area for this threatened population. Finally, our results showcase the benefits of using citizen science to monitor sea turtle populations in easily accessible nearshore habitats.
Effects of Caffeine and Glucose Supplementation at Birth on Piglet Pre-Weaning Growth, Thermoregulation, and Survival
Authors: Jarratt Lillie , James Sarah E. , Kirkwood Roy N. , Nowland Tanya L.
Published: January 2023

Piglet pre-weaning mortality of approximately 15% represents a major economic and welfare concern to the pork industry. Supplementing neonatal piglets with glucose and/or caffeine has the potential to counteract hypoxic stress experienced during parturition and provide an energy substrate, which may improve survival to weaning. This study investigated the effects of caffeine and glucose supplementation at birth, in combination or separately, on piglet growth, thermoregulatory ability, and pre-weaning survival. At birth, 398 piglets were assigned to one of four oral treatments: saline, glucose (300 mg), caffeine (30 mg), or caffeine and glucose combined (30 mg caffeine and 300 mg glucose), dissolved in 6 mL saline. Piglets were tagged at birth, and time taken to reach the udder was recorded. Rectal temperatures were recorded at 4 h and 24 h post-partum, and body weights recorded at birth and 1, 3, and 18 days of age. Colostrum intake was estimated using birth and day 1 weights, and all pre-weaning mortalities were recorded. Treatments did not affect rectal temperature, colostrum intake, or pre-weaning mortality (p > 0.05). Low birth weight piglets (<0.9 kg) treated with caffeine and glucose had increased growth between 1 and 3 days of age (p < 0.05) compared to low birth weight piglets of other treatment groups. Caffeine supplementation alone reduced overall pre-weaning growth in low birth weight piglets compared to all other treatments (p = 0.05). Oral caffeine and glucose had no significant effect on piglet performance except in low birthweight piglets, where it improved growth in the first 3 days of life. Caffeine and glucose supplementation in combination may be beneficial for low birth weight piglets.
Surgical Anatomy for Sterilization Procedures in Female Capybaras
Authors: Jorge Fabiana M. G. , Donoso Flavia Maria Pia Montenegro , Alcobaça Mayla Magalhães de Oliveira , Cristofoli Marilu , Passos Nunes Fernanda B. , Pizzutto Cristiane S. , Assis Neto Antonio Chaves de
Published: January 2023

Capybaras are the largest rodents cohabiting with humans within urban and peri-urban green areas and are known by their prolificity. Surgical contraception has been recommended by official organizations as a way to control capybara populations in areas of zoonotic disease transmission, but little data are available concerning surgical anatomy. To obtain objective anatomical descriptions related to reproductive organs, eight female capybaras cadavers were dissected. The stratigraphy of the lateral (flank) and ventral, post-umbilical (on the linea alba) abdominal wall is described as well as the vascular anatomy of reproductive organs and their syntopy with the abdominal viscera. We commented on the access to the uterine tubes and uterine horns for each approach, and for better description of abdominal wall stratigraphy, abdominal ultrasonography was performed in one live female. All of the animals were provenient from “in situ” population management projects that were properly authorized. Similar abdominal wall stratigraphy was found in comparison to domestic mammals, with emphasis on a thick cutaneous muscle, a thin linea alba, and a large, loose cecum. The uterine tubes were easily accessed by bilateral laparotomy, allowing tubal removal/ligation procedures, while uterine horn exposure was more readily reached by a midline post umbilical celiotomy, favoring horn ligature and hysterotomy techniques. This study can help achieve more efficient contraceptive surgeries in capybaras, reducing the total surgical time and enhancing animal welfare.
Relation between Color and Chemical Composition of Dromedary Camel Colostrum
Authors: El-Hatmi Halima , Oussaief Olfa , Hammadi Imen , Dbara Mohamed , Hammadi Mohamed , Khorchani Touhami , Jrad Zeineb
Published: January 2023

Camel milk industrialization faces technological problems related to the presence of colostrum in milk. The determination of color parameters may serve to differentiate between colostrum and milk. This work aimed to study the relationship between the chemical composition of camel colostrum and milk and their colors. Samples of colostrum were collected at 2, 12, 24, 48, 72, 96, 120, 144, 168, and 360 h postpartum (n = 16), and their physicochemical properties (pH, acidity, viscosity, color, dry matter, ash, protein, and fat) were analyzed. The results show that all the components decreased during the first 3 days except fat. The content of this later increased from zero in the three sampling on the first day (2, 12, and 24 h) to 1.92 ± 0.61% at 48 h postpartum. The amount of total dry matter and protein decreased from 20.95 ± 3.63% and 17.43 ± 4.28% to 13.05 ± 0.81% and 3.71 ± 0.46%, respectively, during the first 7 days postpartum. There was a weak correlation between the brightness (L*) of the camel milk and its contents of dry matter, protein, and fat; however, these parameters were strongly correlated with redness (a*) and yellowness (b*). Ash content was poorly correlated with the color parameters. Hence, the measurement of the color parameters of camel colostrum and milk can be a new tool to evaluate their quality.