bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Multi-walled carbon nanotubes on the performance evaluation of Nickel-based super-alloy–Udimet 720 machined using WEDM process
Authors: Chaudhari Rakesh , Ayesta Izaro , Doshi Mikesh , Khanna Sakshum , Patel Vivek K. , Vora Jay , De Lacalle Luis Norberto López
Published: August 2022

The diverse capabilities of nickel-based super-alloy (Udimet 720), like robust mechanical strength, ductility, resistance to excessive temperature deformation, and advanced corrosion and oxidation resistance, make it suitable for use in multiple applications. These super-alloys are identified as extremely difficult materials for machining to meet feature and manufacturing requirements. In the present work, we demonstrated the machining of Udimet 720 by employing the wire-electrical discharge machining (WEDM) technique. Pulse-on-time (Ton), Pulse-off-time (Toff), current, and MWCNT amount were preferred as input variables. The effect of selected design variables was studied on material removal rate (MRR), surface roughness (SR), and recast layer thickness (RLT). Box Behnken design was utilized to design an experimental matrix. For statistical analysis, analysis of variance (ANOVA) was employed. From ANOVA, the current had the highest contributor with 35.85% to affect MRR, while MWCNT amount was found to be the highest contributor for deciding the values of both SR and RLT with contributions of 42.66% and 40.07%, respectively. The addition of MWCNT at 1 g/L has substantially improved MRR from 0.8546 g/min to 1.2199 g/min, SR reduced from 5.88 µm to 2.98 µm, and reduction in RLT from 17.8 µm to 11.61 µm. The passing vehicle search (PVS) algorithm was implemented, and the results of single-objective optimization presented the largest MRR of 1.8883 g/min, least SR of 1.89 µm, and least RLT of 9.70 µm. Additionally, a set of non-dominated solutions was obtained through Pareto optimal fronts. A small acceptable deviation was detected among the actual and forecasted results from PVS algorithm. It clearly reveals the acceptance of the PVS technique in the present study for Udimet 720. Lastly, the significance of MWCNT amount on surface textures was revealed by employing scanning electron microscopy (SEM).
Hyperactive NK cells in Rag2 knockout mice inhibit the development of acute myeloid leukemia
Authors: Sugimoto Emi , Hayashi Yasutakka , Iida Kohei , Asada Shuhei , Fukushima Tsuyoshi , Tamura Moe , Kawabata Kimihito , Kawase Tatsuya , Saito Takeshi , Yoshida Taku , Yamazaki Satoshi , Kaito Yuta , Imai Yoich , Fukuyama Tomofusa , Tanaka Yosuke , Enomoto Yutaka , Kitamura Toshio , Goyama Susumu
Published: August 2022

Immunotherapy has attracted considerable attention as a therapeutic strategy for cancers including acute myeloid leukemia (AML). In this study, we found that the development of several aggressive subtypes of AML was retarded in Rag2-/- mice despite the lack of B and T lymphocytes, even compared to the immunologically normal C57BL/6 mice. Furthermore, an orally active p53-activating drug showed stronger antileukemia effect on AML in Rag2-/- mice than C57BL/6 mice. Intriguingly, NK cells in Rag2-/- mice were increased in number, highly expressed activation markers, and showed increased cytotoxicity to leukemia cells in a coculture assay. B2m depletion that triggers missing-self recognition of NK cells impaired the growth of AML cells in vivo. In contrast, NK cell depletion accelerated AML progression in Rag2-/- mice. We also found that immunogenicity of AML kept changing during tumor evolution, showing a trend that AMLs with strong leukemogenicity were susceptible to NK cell-mediated tumor suppression in Rag2-/- mice. Thus, our study highlighted the critical role of NK cells in suppressing the development of certain subtypes of AML, and demonstrated that Rag2-/- mice, which are generally considered as “immunodeficient” due to the lack of functional lymphocytes, in fact have hyperactive NK cells with the enhanced antileukemia immunity.
Circulating miR-182-5p for protection of endothelial function from ADMA–induced injury inelderly coronary artery
Authors: Zhang Jiao , Yu Hong , Wang Xiaona , Si Quanjin , Zhao Yangong , Duan Yuanyuan , Ye Ping
Published: August 2022

Background
To investigate the correlation between the expression level of miR-182-5p and endothelial function in elderly individuals.
Methods
One thousand and fifty-eight patients > 60 years old living in the Pingguoyuan area in China were enrolled consecutively and were divided into four groups according to the results of reactive hyperemia index (RHI), and asymmetric dimethylarginine (ADMA). Gruop1 (n = 294):RHI ≥ 1.67 and ADMA ≤ 0.7umol/l (named the normal endotheltial function group); Group2 (n = 244):RHI ≥ 1.67 and ADMA > 0.7umol/l; Group3 (n = 242): RHI < 1.67 and ADMA ≤ 0.7umol/l; Group4 (n = 278):RHI < 1.67 and ADMA > 0.7umol/l(named the endotheltial dysfunction group). The association between miR-182-5p level, RHI and ADMA were analyzed using univariate correlation analysis and multiple regression analysis.
Results
RT-PCR results showed that the expression level of miR-182-5p in the endothelial dysfunction group was significantly lower than that in the normal endothelial function group. In addition,we found that the expression level of plasma miR-182-5p was negatively correlated with plasma ADMA.
Conclusion
MiR-182-5p had a protective effect on endothelial function and may be a potential therapeutic target for atherosclerosis in elderly individuals.
STK33 Promotes the Proliferation and Metastasis of Human Esophageal Squamous Cell Carcinoma via Inflammation Related Pathway
Authors: Wang Hongmei , Huang Lingyan , Li Liping , Ma Tao , Jiang Haifeng , Wang Ruixiao , Chen Xiaozhen , Xu Ke , Chen Chen , Han Zhe
Published: August 2022

The serine/threonine kinase STK33 has been implicated in cancer cell proliferation. Abnormal expression of STK33 is closely related to malignant tumors of many cancers. Here, we provide evidence of a critical role for STK33 in the pathogenesis and metastatic progression of esophageal squamous cell carcinoma (ESCC). In human ESCC specimens, STK33 was overexpressed and associated with poor prognosis. Silencing STK33 expression inhibited ESCC proliferation, migration, invasion, and tumor growth. Furthermore, STK33 was also involved in angiogenesis, TGFB, and inflammatory response in ESCC. Mechanistic investigations showed that STK33 regulated ESCC through multiple complex pathways. In showing that dysregulated STK33 signaling promotes ESCC growth and progression, our results suggest STK33 as a candidate therapeutic target to improve ESCC treatment.
Personalized Sampling Graph Collection with Local Differential Privacy for Link Prediction
Authors: Jiang Linyu , Yan Yukun , Tian Zhihong , Xiong Zuobin , Han Qilong
Published: August 2022

Link prediction (LP) is an attractive research problem on social network data. Yet, the link information may be leaked by the untrusted collector. As countermeasures, there are a few methods specially designed for link prediction under local differential privacy (LDP), which allow the third-party data collector collects user data for link prediction while protecting user connection privacy. In this paper, we propose a Community-based graph collection with Personalized sampling Randomized Response (CPRR) which is a novel graph collection algorithm with LDP and reaches decent trade-off between sensitive link protection and link prediction performance. The proposed mechanism adopts a personalized sampling technique for each connection of a user and then utlizes the randomized response mechanism on the sampled subset. Based on the personalized sampling technique, we can reduce the injected noise in LDP. Meanwhile, considering the different edge distributions of the different regions on the original graph, we propose a community-based sampling strategy. Then, we prove that the proposed CPRR satisfies LDP. %We compare the performance of CPRR and other privacy-preserving graph collection methods under LDP in terms of link prediction accuracy. Through extensive experiments on several real-life graph datasets, we demonstrate that CPRR can achieve better results in balancing privacy protection and link prediction performance than the state-of-art baselines
Seismic collapse risk of RC-timber hybrid building with consideration of NBCC 2020 seismic hazard model
Authors: Odikamnoro Ikenna , Badal Prakash S. , Burton Henry , Tesfamariam Solomon
Published: August 2022

The latest seismic hazard model described in the 2020 National Building Code of Canada (NBCC) marks a comprehensive improvement over its predecessor (NBCC 2015). For different regions in Canada, this will have an impact on the design of new buildings and performance assessment of existing ones. In the present study, this impact is quantified for a recently developed hybrid building system of cross-laminated timber (CLT) and reinforced concrete (RC) with steel slit dampers. The six-story CLT-RC dual frame, designed using the direct displacement-based method, is located in Vancouver, Canada. Along with a very high seismicity, southwestern British Columbia is characterized by complex seismotectonics consisting of subduction, shallow crustal, and in-slab faulting mechanisms. With the NBCC 2015 and 2020 models, a hazard-consistent set of 40 pairs of ground motion records is selected from the PEER and KiK-net databases, and used to estimate the building’s seismic risk performance. The collapse capacity of the building reveals the need for the use of site-specific ground motion selection for sites with a complex seismicity. The results also indicate that the combination of CLT and steel slit dampers enhances the seismic performance of the hybrid system. The probability of collapse at a hazard level corresponding to a 2475 year return period event is estimated to be 2.7%.
Recurrent triple-negative breast cancer from cysteine deprivation loses tumorigenicity via downregulation of the CST4 signaling
Authors: Tang Xiaohu , Alothaim Tahiyat , Charbonneau Morgan
Published: August 2022

Triple-negative breast cancer (TNBC) is an aggressive type of breast cancer with a high risk of recurrence following therapeutic treatments. Targeted cysteine therapy via inhibition of cysteine uptake by erastin effectively induces mesenchymal TNBC cells to ferroptosis. However, a small residual population of cancer cells exhibited the erastin-resistance and survived after the erastin treatment. This phenomenon is likely analogous to the event of tumor recurrence in patients after therapy. To characterize this resistance, we established the erastin-resistant/recurrent TNBC cell models in vitro by multi-cycles of erastin challenge. By an epigenetic compound library screen, the erastin-resistance be abolished by adjuvant epigenetic compounds. Intriguingly, the erastin-recurrent TNBC cells failed to grow in anchorage-independent conditions, implying a loss of tumorigenicity. By transcriptomic profiling analysis, the recurrent cells displayed many gene expression alterations and attenuated signaling processes, including K-Ras signaling. Three members of the cystatin gene family, including CST4, were significantly downregulated in recurrent cells. Knocking out of CST4 by CRISPR/Cas9 significantly suppressed the tumorigenic potential and K-Ras signaling. Our findings suggested that targeted cysteine therapy could be a valid treatment for mesenchymal TNBC with a low probability of tumor recurrence.
Evaluation of the long-term endurance of reinforced concrete structures
Authors: Boukari Wadjidou , Abdou-Latif Imorou
Published: August 2022

Even though experts had been monitoring infrastructure quality previous to the COVID-19 epidemic, they had not normally widely highlighted the importance of constructing high-quality infrastructure. The lifespan of a structure is a significant consideration in evaluating how long it will last. This, however, necessitates a reevaluation of infrastructure durability to promote more sustainable growth. Based on these facts, this paper presents a method for measuring building durability utilizing structure deformation effects. The findings indicate that building durability will be acceptable in cities if building deformations in the serviceability state (SLS) do not exceed those of the ultimate limit state (ULS).
Prognostic Value of Lactate/albumin Ratio for Mortality in Patients With Complicated Urinary Tract Infection
Authors: Alberola Juan , Eiros José María , Artero Arturo , Madrazo Manuel , López-Cruz Ian , Piles Laura , Mico Jaume
Published: August 2022

Background
Lactate to albumin ratio (LAR) is an emerging sepsis biomarker that has been tested for mortality in patients with sepsis of different focus. Our goal is to evaluate the prognostic value of LAR in patients admitted to hospital due to complicated urinary tract infection.
Methods
Prospective observational study of patients older than 65 years diagnosed with UTI. Area under the ROC curve, sensibility, and specificity to predict 30-day mortality were calculated for LAR, qSOFA and SOFA and compared.
Results
341 UTI cases were analyzed. 30-day mortality (20.2% vs. 6.7%, p < 0.001) and longer hospital stay (5 [4–8] vs. 4 [3–7], p 0.018) were associated with LAR ≥ 0.708. LAR has no statistically significant differences compared to qSOFA and SOFA for predicting 30-day mortality (AUROC 0.737 vs. 0.832 and 0.777 respectively, p 0.119 and p 0.496). The sensitivity of LAR was similar to the sensitivity of qSOFA and SOFA (60.8% vs. 84.4% and 82.2, respectively, p 0.746 and 0.837). However, its specificity was lower than the specificity of qSOFA (60.8% vs. 75%, p 0.003), but similar to the specificity of SOFA (60.8% vs. 57.8%, p 0.787).
Conclusion
LAR has no significant differences with other well-stablished scores in sepsis, such as qSOFA and SOFA, to predict 30-day mortality in patients with complicated UTI.
Environmental DNA (eDNA) assays for the detection of Ridgway’s Rail (Rallus obsoletus)
Authors: Guan Xin , Lance Richard F. , Feist Sheena M.
Published: August 2022

We designed two novel environmental DNA (eDNA) assays for the detection of Ridgway’s Rail (Rallus obsoletus), and successfully validated each assay using eDNA samples collected from the species’ known distribution within the United States. These assays add to the suite of tools available for the monitoring of this rare and secretive marsh bird, and may help to further elucidate its movement patterns as well as identify important migration corridors. Observed sensitivity of the assays indicates exceptional performance, with limits of detection at ≤ 8 copies of the target eDNA fragment per reaction. Our publication adds to the growing body of literature supporting eDNA surveys as viable tools for bird monitoring endeavors.
Fludarabine/TBI 8 Gy versus fludarabine/treosulfan conditioning in patients with AML in first complete remission: A Study from the Acute Leukemia Working Party of the EBMT
Authors: Bug Gesine , Labopin Myriam , Niittyvuopio Riitta , Stelljes Matthias , Reinhardt Christian , Hilgendorf Inken , Kroeger Nicolaus , Kaare Ain , Bethge Wolfgang , Schaefer-Eckart Kerstin , Verbeek Mareike , Mielke Stephan , Carlson Kristina , Bazarbachi Ali , Spyridonidis Alexandros , Savani Bipin , Nagler Arnon , Mohty Mohamad
Published: August 2022

The optimal reduced intensity conditioning (RIC) regimen is a matter of debate. We retrospectively compared conditioning with fludarabine plus fractionated total body irradiation of 8 Gy (FluTBI) and fludarabine plus treosulfan 30, 36 or 42 g/m2 (FluTreo) in 754 patients with AML above the age of 40 years undergoing an allogeneic hematopoietic stem cell transplant (HSCT) in first complete remission (CR). In a multivariate analysis, FluTBI was associated with a significantly lower probability of relapse than FluTreo (hazard ratio (HR) 0.59 [95% CI, 0.38–0.93], p = 0.023). There was no significant difference in leukemia-free survival (LFS), overall survival (OS), graft-versus-host disease-free and relapse-free survival (GRFS), or acute and chronic graft-versus-host disease (GVHD). After balancing patient characteristics by propensity score matching of 115 patients in each group, FluTBI retained its significantly lower probability of relapse compared to FluTreo (18.3% vs. 34.7%, p = 0.018) which was counteracted by a higher non-relapse mortality (16.8 vs. 5.3%, p = 0.02). Thus, OS and GRFS at 2 years were similar between groups (OS 66.9% vs. 67.8%, GRFS 50.3% vs. 45.6%). We conclude that both conditioning regimens are effective and safe, but FluTBI may better be reserved for younger patients below the age of 55 years.
Spatial and Temporal Effects of Wind Speed on Global Vegetation Growth
Authors: Li Zhiwei , Fu Lijiang , Guo Wei , Song Li , Tan Jinglu , Tang Hao , Xu Zhenyu , Guo Ya
Published: August 2022

Wind effects on vegetation growth have not been examined at the global scale. In this work, the spatial and temporal effects of wind speed on global Solar-induced Chlorophyll Fluorescence (SIF) and Gross Primary Production (GPP) were analyzed. The results show: (1) Spatially, wind has almost the same area percentage (about 12%) of positive influence on SIF (GPP) no matter SIF (GPP) changes or not. Wind itself explains the negative impact more than it does the positive impact. (2) Temporally, there is significant increase in the area percentage (0%~3.9% each year) and magnitude of positive wind effects on SIF and GPP globally for most of vegetation types from 2001 to 2018. (3) Based on SIF and GPP, different types of vegetations enjoy different level of optimal wind speeds. This work provides an overall picture of wind speed effects on vegetation growth globally, and are important for understanding sustainability while climate changes.
Synthesis and Characterization of Composites from Phoenix dactylefera L. Fibers
Authors: BOUGHZEL Abdeslam , LANEZ Touhami , Mya Omar Ben
Published: August 2022

Date palm (Phoenix dactylefera L.) fibers, which are natural wastes, have been recovered and exploited in the synthesis of composite materials by mixing them with two different polymers: polyvinyl chloride (PVC) and polystyrene (PS). The four samples were characterized using infrared spectroscopy (FTIR), water absorption rate (W) measurements, thermal conductivity (λ), and mechanics (tensile stress and stretching at break) testing. The results proved that: (i) At 40°C, the composites absorption rate was higher than at the room temperature. While this rate was better for the composites with non-alkali-treated fibers; (ii) to obtain a thermally insulating composite, polystyrene polymer should be added to the matrix and the alkaline treatment of the used vegetable fibers should be required. (iii) Composites made of non-alkali treated fibers have a high stretching at break values while composites made of alkali-treated fibers have a high tensile stress value.
Selection of Operative Approach in Children with Currarino Syndrome
Authors: Pang Wenbo , Chen Yajun , Wang Kai , Zhang Dan , Qi Xiang , Wu Dongyang
Published: August 2022

Purpose: To summarize the experience of surgical treatment of children diagnosed with Currarino syndrome, with an emphasis on selection of optimal operative approach. Methods: The clinical materials of patients diagnosed with Currarino syndrome were recorded. Special attention was paid to the operative management, particularly the different routes for operation. The type of ARM was the critical point. Follow-up was conducted for at least 3 months, and the Rintala score was used for evaluation of bowel function.Results: The medical records of 26 patients were reviewed and listed in table one. Seven were male, and 19 were female, with a mean age of 19.38±13.80 months (ranging from 2 to 47 months). The anorectal malformation included 17 rectoperineal fistulae without an anus, four rectovestibular fistulae without an anus, two anal stenoses, one anal atresia, one rectourinary fistula without an anus, and one cloaca. The PS was taken in 23 cases. The SPS was taken in eight cases, and LPS was taken in another 15 patients. SPS group included three rectoperineal fistulae without an anus, one anal stenosis, one retraction of the rectum after an anoplasty for rectovestibular fistula without an anus, one anal atresia, one rectourinary fistula without an anus, and one cloaca. In the LPS group, there were 13 perineal fistulae without an anus，one displacement of the rectum and one retraction of the rectum respectively after an anoplasty for rectovestibular fistula without an anus. TA was used in one anal atresia with megacolon and presacral mass. AS was used in one rectovestibular fistula with a presacral mass. A lamina approach was chosen for one patient with an infective fistula secondary to a presacral mass in the spinal canal. The mean follow-up time was 39.48±26.84m, ranging from 6m to 145m. The Rintala score was16.74±2.93, ranging from 12 to 20.Conclusion: For a rectoperineal fistula, SPS or LPS could be used to reach a complete cutback of the posterior wall of the rectum and remove the presacral mass. For a rectovestibular fistula, the AS or LPS could be chosen. For anorectal stenosis, TA, SPS, or LPS could be used to excise the stenosed bowel segment and the presacral mass. TA is not so good for the exposure of the presacral mass, so for a larger one(＞5cm) or one connecting with the spinal canal, the LPS or SPS may be the favorite choice.
Volumetric measurement of intracranial meningiomas: a comparison between linear, planimetric, and machine learning with multiparametric voxel-based morphometry methods
Authors: Silva Jonadab dos Santos , Schreiner Cláudia Abib , de Lima Lázaro , Brigido Carlos Eduardo Pinheiro Leal , Wilson Christopher D , McVeigh Luke , Acchiardo Joseph , Landeiro José Alberto , Acioly Marcus André , Cohen-Gadol Aaron
Published: August 2022

Purpose
To compare the accuracy of three volumetric methods in the radiological assessment of meningiomas: linear (ABC/2), planimetric, and multiparametric machine learning-based semiautomated voxel-based morphometry (VBM), and to investigate the relevance of tumor shape in volumetric error.
Methods
Retrospective imaging database analysis at the authors’ institutions. We included patients with a confirmed diagnosis of meningioma and a volumetric acquired cranial magnetic resonance imaging. After tumor segmentation, images underwent automated computation of shape properties such as sphericity, roundness, flatness, and elongation.
Results
Sixty-nine patients (85 tumors) were included. Tumor volumes were significantly different using linear (13.82 cm³ [range: 0.13–163.74 cm³]), planimetric (11.66 cm³ [range: 0.17–196.2 cm³]) and VBM methods (10.24 cm³ [range: 0.17–190.32 cm³]) (p < 0.001). Median volume and percentage errors between the planimetric and linear methods and the VBM method were 1.08 cm³ and 11.61%, and 0.23 cm³ and 5.5%, respectively. Planimetry and linear methods overestimated the actual volume in 79% and 63% of the patients, respectively. Correlation studies showed excellent reliability and volumetric agreement between manual- and computer-based methods. Larger and flatter tumors had greater accuracy on planimetry, whereas less rounded tumors contributed negatively to the accuracy of the linear method.
Conclusion
Semiautomated VBM volumetry for meningiomas is not influenced by tumor shape properties, whereas planimetry and linear methods tend to overestimate tumor volume. Furthermore, it is necessary to consider tumor roundness prior to linear measurement so as to choose the most appropriate method for each patient on an individual basis.
Anti-neuroinflammatory effects and brain pharmacokinetic properties of selonsertib
Authors: Lee Ji Hun , Ji Sang Hee , Lim Jong Seung , Ahn Sunjoo , Yun Hwi-yeol , Kim Seong Hwan , Song Jin Sook
Published: August 2022

Selonsertib is a first-in-class apoptosis signal-regulating kinase 1 (ASK1) inhibitor that was in clinical trials for treatment of NASH and diabetic kidney disease due to its anti-inflammatory and anti-fibrotic activities. In the present study, we investigated the anti-neuroinflammatory effects and brain pharmacokinetic properties of selonsertib. It inhibited TNF-α and NO production by suppression of phosphorylated ASK1 in the LPS-stimulated microglial cell line, BV2 cells. Consistent with the in vitro results, selonsertib attenuated plasma and brain TNF-α levels in the LPS-induced murine neuroinflammation model. In vitro and in vivo pharmacokinetic studies of selonsertib were conducted in support of central nervous system (CNS) drug discovery. In both Caco-2 and MDR-MDCK cells, selonsertib exhibited a high efflux ratio, showing that it is a P-gp substrate. Selonsertib was rapidly and effectively absorbed into the systemic circulation after oral treatment, with a Tmax of 0.5 h and oral bioavailability of 74 %. In comparison with high systemic exposure with Cmax of 16.2 µg/ml and AUC of 64 µg·h/mL following oral dosing of 10 mg/kg, the brain disposition of selonsertib was limited, with Cmax of 0.08 µg/g and Kp value of 0.004. This study demonstrates that selonsertib has the potential to be used as a therapeutic agent for neuroinflammatory diseases.
Comparison of Malignancy and Spatial Distribution between Latent and Clinical Prostate Cancer--an eight-year biopsy study
Authors: Liang Zhen , Zhou Zhien , Huang Hanzi , Wu Xingcheng , Xiao Yu , Wang Wenze , Zuo Yuzhi , Chen Yuliang , Zhou Yi , Yan Weigang
Published: August 2022

BackgroundCurrent prostate cancer (PCa) screening may detect nonprogressive lesion, leading to overdiagnosis and overtreatment. The purpose of present study is to investigate whether the tumor pathological origin of latent prostate cancer (lPCa) and clinical prostate cancer (cPCa) is consistent, and to verify the current clinically significant prostate cancer criteria.Methods:Prostate specimens were obtained from postmortem autopsy between 2014 and 2021 and patients who went through radical prostatectomy from 2013 to 2021. The pathological characteristics and spatial distribution of the lPCa group and cPCa were compared and analyzed through SPSS software with P < 0.05 representing statistical significant. ResultsIn lPCa group, a total of 45 tumor lesions from 24 lPCa cases were included, 54.2% of lPCa patients were ISUP ≥ 2, 12.5% had tumor volume ≥ 0.5 ml, and 16.7 % had extraprostatic extension (EPE). In cPCa group, there were a total of 429 tumor lesions in 126 cases, 92.1% of cPCa patients were ISUP ≥ 2, and 82.5% had tumor volume of ≥ 0.5ml. 56.3% had EPE. LPCa and cPCa have the same spatial distribution characteristics, no significant difference was detected between the anterior and posterior zone. Peripheral zone tumors were significantly more common than transitional zone tumors. Tumors in apical 1/3 and middle 1/3 were significantly more common than basal 1/3. ConclusionThe malignancy of cPCa is significantly higher than that of lPCa, and the spatial distribution of cPCa and lPCa are consistent. ISUP grade 2 is not sufficient to determine clinical significance of tumor.
Workload Indicators of Staffing Need (WISN) Method for
Authors: Asres Gizew Dessie
Published: August 2022

Background
Work force is a crucial component of health services delivery system. Ethiopia faces health workforce challenges with regard to evidence based health work force planning. First the health worker to population ratio was used and later standard staffing schedule for each health facility level was used. Both of these methods did not address the issue of evidence based workload variation between the same levels of facilities at different locations within a country. A workload indicator of staffing need method (WISN) addresses these variations. Therefore this study was assumed for recommendation on use of WISN for health facilities based on WISN results of midwives at Asrade Zewude memorial Hospital.
Methods
Cross sectional study using WISN model was used to determine the gaps or excess and workload pressure in midwives at Asrade Zewude Memorial primary hospital, North West Ethiopia.
Results
The finding showed that there was five working days with 1030 hrs actual working time per year for midwives. This working time was spent on health service activities (58.4%), additional activities (36.6%) and support activities (5%). WISN calculation showed that a shortage of five midwives with WISN ratio of 0.8 at Asrade Zewude Memorial primary hospital North West Ethiopia.
Conclusion
Midwives at the study area were doing their routine work with 20% under staffed by covering the additional five midwives' position. With this working condition, it may be hard to achieve universal health coverage goals of the facility. Therefore the hospital should institutionalize WISN method to objectively employ midwifery professionals.
Exploring Natura 2000 Habitats by Satellite Image Segmentation: a case study from the Čierny Balog area (Central Slovakia)
Authors: Čahojová Lucia , Ambroz Martin , Jarolímek Ivan , Kollár Michal , Mikula Karol , Šibík Jozef , Šibíková Mária
Published: August 2022

Natura 2000 is a network of protected areas covering Europe's most valuable and threatened species and habitats. Recently, biota belonging to these networks have been threatened by both climate change and various human impacts. Regular monitoring is needed to ensure effective protection and proper management measures in these sites and habitats, but conventional field approaches are often time-consuming and inaccurate. New approaches and studies with different focuses and results are being developed. Our approach includes point data from field research and phytosociological databases as starting points for automatic segmentation, which has been developed just recently as a novel method that could help to connect ground-based and remote sensing data. The main aim of our case study is to apply advanced remotely sensed techniques to map the area and condition of vegetation units. We focus on forest habitats belonging mainly to the Natura 2000 network in the area of Čierny Balog village (Central Slovakia). We concentrated on the verification of the possibilities of differentiation of various habitats using only multispectral Sentinel-2 satellite data. New software created by our team called NaturaSat was used to reach our objectives. In the identified areas, spectral characteristics were calculated using software tools, which were subsequently processed and tested statistically. We obtained significant differences between forest habitat types that provided promising results and verification of the methods used. This type of new habitat identification is necessary for the automatic monitoring of habitat areas and changes in conditions by remote sensing.
Intertwining conditions for two isometries on Banach space
Authors: Mustafayev Heybetkulu
Published: August 2022

In this note, we present some results concerning intertwining properties of two isometries on Banach space. In this connections, we obtain also some Katznelson-Tzafriri type results for power bounded operators.
CRISPR screens reveal neuropeptide signaling orchestrates T helper cell differentiation
Authors: Kuchroo Vijay , Hou Yu , LaFleur Martin , Huang Linglin , Lambden Conner , Thakore Pratiksha , Geiger-Schuller Kathryn , Tang Ruihan , Shi Jingwen , Barilla Rocky , Subramanian Ayshwarya , Wallrapp Antonia , Choi Hee Sun , Kye Yoon-Chul , Ashenbrg Orr , Schiebinger Geoffrey , Doench John , Regev Aviv , Sharpe Arlene
Published: August 2022

The balance between T helper type 1 (Th1) cells and other Th cells is critical for antiviral and anti-tumor responses, but how this fine balance is achieved remains poorly understood. Here, we dissected the dynamic regulation of Th1 cell differentiation during in vitro polarization, as well as in vivo differentiation upon acute viral infection, using scRNA-seq and multiple in vitro and in vivo CRISPR screens. We confirmed the role of known regulators and identified 5 novel regulators for Th1 differentiation. Among the novel regulators the neuropeptide receptor RAMP3, which is a component of the receptor for calcitonin gene-related peptide (CGRP), plays a cell-intrinsic role in Th1 cell-fate determination. Using a unique Th1/Th2 dichotomous culture system, we identified that RAMP3-CGRP interaction directly restricted the differentiation of Th2 cells but promoted Th1 differentiation through activation of downstream cyclic AMP (cAMP) signaling in T cells. Mechanistically, RAMP3 and cAMP signaling resulted in global changes in chromatin accessibility, blocking Th2 genes and specific induction of Th1 programs through activation of IFNγ-STAT1 pathway. Furthermore, both CGRP and RAMP3 were required for inducing effective anti-viral T cell responses to control acute viral infection. Our work reveals a novel neuro-immune circuit in which tissue itself participates in T cell fate determination by producing a neuropeptide during acute viral infection, which acts on RAMP3 expressing T cells to induce an effective anti-viral Th1 response.
Effect of non-protein amino acids β-amino butyric acid (BABA) on the intestinal G- bacterial community
Authors: Al-Dulaimy Eman Ahmed , Jasim Mohammed Abbas
Published: August 2022

This study was conducted in the animal house of the College of Education for Girls, to investigate the effect of beta-amino butyric acid (BABA) on the microorganisms in the digestive system of mice. The bacteria present in the stool were isolated and diagnosed. And that intestinal bacteria have an effective role in influencing human health. However, few factors that affect the bacteria in the digestive system are known. Despite the increase in the number of microbial communities colonizing the gastrointestinal tract, many of these bacteria have been found to possess virulence factors that can negatively affect the host organism and protect it from prevailing antibiotics. BABA) was used with four treatments (100, 200, 300, 0) mg/kg animal weight denoted by A, B, C, and D. Male rats were immersed orally in four doses per week, and the results showed that the amino acid effectively affected On the intestinal bacterial community, where the third concentration of the fourth dose gave the lowest rate of the number of bacterial isolates, which led to the elimination of a large number of them, followed by the second concentration and then the first compared to the comparison treatment that achieved the highest rate of the number of isolates. While histidine had no negative effect on the Toll-Like Receptor 4 (TLR4), the results showed that TLR4 was a normal receptor.
Species interactions, stability, and resilience of the gut microbiota - helminth assemblage in horses
Authors: Boisseau Michel , Dhorne-Pollet Sophie , Bars-Cortina David , Courtot Élise , Serreau Delphine , Annonay Gwenolah , Lluch Jérôme , Gesbert Amandine , Reigner Fabrice , Sallé Guillaume , Mach Núria
Published: August 2022

The nature and strength of interactions entertained among helminths and their host gut microbiota remain largely unexplored.
Using 40 naturally infected Welsh ponies, we tracked the gut microbiota-cyathostomin temporal dynamics during parasite community removal and reassembly, and the associated host immune response. Infected ponies harboured 14 species of cyathostomins, overwhelmed by the abundance of Cylicocyclus nassatus. Parasite carriers exhibited gut environment modifications, higher Shannon entropy and orderly rearrangements of prokaryotic assemblages, with protective Clostridia species contributing to the successional nemabiome-microbiota crosstalk. Yet, the gut ecosystem was remarkably stable, and the host systemic response defined enrichment for B-cell activation and IgA production without corresponding changes in parasite burdens. Therefore, Clostridia microbial protection likely reduced fluctuating dynamics between the microbiota-parasite-host triad and favoured parasite tolerance.
The system stability was disrupted by pyrantel treatment and parasite removal, with dire early consequences on the gut environment, microbiota diversity, and cytokine networks while highlighting the detrimental effect of cyathostomin burdens on Enterococcus spp. Both ecological communities were highly resilient to disturbance and recovered their pre-treatment compositions but for Cylicostephanus longibursatus in the parasite community. However, gut microbiotas failed to restore their original stability and shifted towards an interacting unstable state, with transient coexistence between Clostridia and core bacterial taxa, e.g. Fibrobacter and Prevotella, evoking their crucial role as stabilising forces for this new equilibrium.
These observations highlight how anthelmintic treatment alters the gut microbiota stability and open new perspectives for adding nutritional intervention to current parasite management strategies in the field.
Generalization of Quartic and Quintic Calabi – Yau Manifolds Fibered by Polarized K3 Surfaces
Authors: Bhattacharjee Deep
Published: August 2022

I will present Calabi-Yau manifolds emphasizing quartic and quintic variety considering Bogomolov-Gieseker Inequality with two specific types of Clifford inequality for associated fibers by Polarized K3 surfaces with the case considered upon threefolds. Generalizations have been made to a different form of quintics with coordinates and associated mirrors for the hypersurface being concerned with taking multi-homogeneous polynomials where necessary. Minimal Calabi-Yau threefold considered for fiber varieties I0, I+, II0, II+, III, 0.
Selective Image segmentation driven by region, edge and saliency functions
Authors: Soomro Shafiullah , Soomro Toufique Ahmed , Niaz Asim , Choi Kwang Nam
Published: August 2022

In this paper, we propose a novel level set method based on selective homogeneous and inhomogeneous object segmentationdriven by an image region, edge and saliency functions. Initially, we adapt an intensity edge term based on the zero crossingfeature detector (ZCD), which is used to highlight significant edges of an image. Secondly, a saliency function is formulatedto detect salient regions from an image. We have also included a globally tuned region based SPF (signed pressure force)term to move contour away and capture homogeneous regions. ZCD, saliency and global SPF are jointly incorporated withsome scaled value for the level set evolution to develop an effective image segmentation model. Moreover, proposed method iscapable to perform selective object segmentation, which enables us to choose any single or multiple objects inside an image.Saliency function and ZCD detector are considered feature enhancement tools, which are used to get important features of animage, so this method has a solid capacity to segment nature images (homogeneous or inhomogeneous) precisely. Finally, theadaption of the Gaussian kernel removes the need of any penalization term for level set reinitialization. Experimental resultswill exhibit the efficiency of the proposed method.
Does brand personality mediate the link between social media usage and customer buying decisions on telecommunication’s products and services? Evidence from Ghana
Authors: Attor Cleophas , Bashiru Jibril Abdul , Chovancová Miloslava
Published: August 2022

Social media marketing has vastly benefitted businesses, including the development of brand identity via solid communication channels. Innovative marketing tools have proven effective among service and non-service-based businesses. This study examines the impact of social media on customer buying decisions via brand personality attributes among telecom products (e.g., mobile phones, sim cards, and data subscriptions) in Ghana. A positivist research paradigm with a non-probability sampling were deployed to achieve study goals. A structured questionnaire was designed to collect the data from subscribers of the telecom giants (MTN, Vodafone, and Airtel-Tigo) in the capital of Ghana through a non-probability sampling technique (snowball/referral method). Both self-administered and online survey (link) were deployed with strict adherence to Covid-19 protocols. A valid data set of 414 (representing 82% of response rate) from 507 responses was received for data processing. PLS-SEM was applied to analyze the study hypothesis. The study identified five main brand personality attributes (i.e., brand sincerity, brand excitement, brand competence, brand sophistication, and brand ruggedness) as mediators of the proposed framework. The study found that brand sincerity, brand excitement, and brand competence played a significant mediation effect on the relationship between social media usage and customer buying decisions, whereas brand sophistication and brand ruggedness did not. The findings suggest that brand management plays an interconnected role in customer decision-making in which brand practitioners should take a keynote regarding their strategic marketing decisions. Finally, the paper recommends that future research consider a mixed approach to offer an in-depth analysis.
Acknowledgment This study is supported by Tomas Bata University in Zlin through IGA/FAME /2022/010 Influencer marketing and intercultural differences across generations and IGA/FAME/2021/005-Significant factors in the sustainability of economic growth with a focus on the SME segment. We are grateful to the Editor-in-Chief and the anonymous reviewers for their comments in shaping this manuscript.
Fit-for-Purpose MIP OES Method to Meet the Requirements of the Brazilian Regulations for K and Na in Coconut Water, and Nutritional Assessment
Authors: de Menezes Larissa , Lorençatto Rodolfo , Peixoto Rafaella , Duyck Christiane , Rocha Anderson
Published: August 2022

The Brazilian regulations for processed coconut water establish the range concentrations of Na and K, being necessary high-throughput analytical methods, as microwave-induced plasma optical emission spectrometry (MIP OES) for quality control. Samples of natural (green and mature) and processed coconut water were treated by acid decomposition assisted by microwave radiation before their MIP OES analysis. Recoveries for Na and K were 103 and 95%, whereas the relative standard deviation (RSDs) were 7.6 and 1.3%, respectively. The limit of detection (LOD) was 1.77 mg kg-1 for K and 2.31 mg kg-1 for Na. Sodium and K were determined in the ranges of, respectively, 27-490 and 1,600-3,500 mg L-1 in natural samples, whereas in processed samples the ranges were 26-168 and 814-2,054 mg L-1. K-means cluster analysis identified a group of processed samples with compositions similar to green coconut water. All the processed samples were in accordance with the established regulation, except one dehydrated sample for which K content was below the accepted range. Natural coconut water is an excellent source of K in the diet as its daily consumption can supply more than 20% of the recommended daily ingestion of K for adults and 30% for children.
Penerapan Ghinā’ ʿArabī sebagai Kegiatan Penunjang Giat Berbahasa Arab Siswa MTs NU Nurul Huda Semarang
Authors: Hanifah Hidayati Nurul
Published: August 2022

This article aims to describe the application of ghinā’ ʿarabī as an activity to support active Arabic language students at MTs NU Nurul Huda Semarang. The use of the ghinā’ method is considered more effective than other methods in supporting active Arabic students, especially for MI and MTs students. The method used in this study is a qualitative descriptive method. The data collection techniques were obtained through observation, interviews, and documentation. The application of ghinā’ ʿarabī to support the habituation of students' active learning at MTs NU Nurul Huda Semarang is very effective. Students become more enthusiastic and easier to understand and learn Arabic. This method also helps teachers in overcoming the lack of student focus in learning. In addition, students and teachers can also carry out teaching and learning activities in a conducive and fun way.
Proximal composition, fatty acid profile, omegas and lipid quality in the tambaqui (Colossoma macropomum Cuvier, 1818) (Serrasalmidae) in “flatted cut”
Authors: Cavali Jucilene , Francisco Reginaldo da Silva , Dantas Filho Jerônimo Vieira , Pontuschka Rute Bianchini , Silva Tiago Lucena da , Amaral Rodrigo Vieira Alves
Published: August 2022

The aimed of this study was to determine proximal composition, fatty acid profile, omegas and lipid quality in the tambaqui (Colossoma macropomum) in “flatted cut”, produced and commercializeded by Rondônia state, Brazil. Three pieces of flatted tambaqui were collected from 40 fish weighing 1.10 ± 0.10 kg. Proximal composition were determined by AOAC Official method 969.23 and 968.08. The fatty acids were grouped to calculate ratio ∑PUFAs/∑SFAs and proportion ∑PUFAs (n-6/n-3), atherogenicity index (AI) were calculated, thrombogenicity index (TI), and ratio between hypocholesterolemic and hypercholesterolemic fatty acids (HH). Were found 1.06 g/100g of mineral matter, 17.64 g/100g of crude protein, 5.74 g/100g of lipids, 75 .54 g/100g of moisture and 122.22 kcal/100g of caloric value. Lipid composition 39.425% of SFAs, 43.518% of MUFAs and 17.057% of PUFAs. It also presented ∑PUFAs/∑SFAs ratios of 2.31 and ∑PUFAs (n-6/n-3) of 3.78. As AI of 0.44, TI of 0.92 and HH of 2.02. Tambaqui meat of 1.10 ± 0.10 kg in flatted cut contains good proximal contents, with high protein content and excellent lipid content, being considered a low-fat meat, with low moisture content and low caloric value and rich in minerals. It can also be considered a good source of MUFAs and PUFAs, especially n-3 and n-6, with high nutritional value, in addition to essential fatty acids, ALA, AA, DHA and EPA, which were crucial to provide good levels of lipid quality.
Use of ozone in the culture medium of zebrafish (Danio rerio) embryos
Authors: Virote Bárbara do Carmo Rodrigues , Martimiano Daniel Vitor , Fabem Sarah Lacerda , Tanaka Marcelo Stefanini , Locha Letycia Andrade , Murgas Luis David Solis
Published: August 2022

In this study, we sought to demonstrate the toxicity of different concentrations of ozonated water in zebrafish embryos up to 120 hours postfertilization (hpf ) and their antimicrobial activity advantages. For the test, we placed 40 embryos per treatment in Petri dishes containing 30 ml of solution to be tested. The ozone concentrations used were calculated from an initial concentration of 72 μg/ml using the O&L 1.5 RM ozone generator (Ozone & Life, Brazil). Five serial dilutions 1:1 was performed in egg water to produce the ozone treatments. We also analyzed a treatment with 30 ml of egg water without ozone, with only the addition of methylene blue, and a treatment with 30 ml of egg water without the addition of any antifungal agent. The plates were incubated at 28 ± 1°C, and the embryos were analyzed daily until 120 hpf. The survival rate, incubation period, and possible deformities were analyzed. In addition, egg water microbiological analyses were performed to detect total coliforms and fungi and yeasts. The data were tested for normality, and analysis of variance was performed within Minitab®version 1.8 software. The results showed that there was no significant difference in embryo survival or hatching rate between treatments with different ozone concentrations (p > 0.05), and no embryonic deformities were found under any of the ozone concentrations evaluated. It can be concluded that ozone within the concentrations analyzed is not toxic to zebrafish embryos and has antifungal and antimicrobial action.
Strategi Humas Meningkatkan Partisipasi Masyarakat dalam Kegiatan Sekolah
Authors: Prabandari Diah Ayu , Supriyanto Ahmad , Sobri Ahmad Yusuf , Fadhli Rahmat
Published: August 2022

Tujuan penelitian ini yaitu mendeskripsikan kegiatan humas dalam meningkatkan partisipasi masyarakat. Penelitian ini menerapkan metode penelitian kualitatif. Penelitian dilakukan di Sekolah Menengah Pertama An-Nur Bululawang Kabupaten Malang. Teknik pengumpulan data dilakukan dengan wawancara, observasi serta dokumentasi. Teknik analisis data mengikuti model analisis data kualitatif Miles dan Huberman. Keabsahan data dicapai dengan triangulasi data. Hasil penelitian menunjukkan bahwa pelibatan masyarakat dalam kegiatan sekolah dilakukan melalui kegiatan rutin dan kegiatan insidental. Media yang digunakan untuk berkomunikasi dengan masyarakat yaitu WhatsApp dan surat formal. Langkah kegiatan yang dilakukan meliputi perencanaan, pelaksanaan, dan evaluasi kegiatan. Hasilnya yaitu citra sekolah yang baik serta terbangunnya komunikasi positif antara sekolah dengan masyarakat. Kendala yang dihadapi sekolah dalam meningkatkan partisipasi masyarakat yaitu kesibukan orang tua siswa.