bluuebunny/crossref_metadata_2025 huggingface dataset:
Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico
Authors: Al-Rajhi Aisha M. H. , Qanash Husam , Bazaid Abdulrahman S. , Binsaleh Naif K. , Abdelghany Tarek M.
Published: April 2023

The resistance of cancer and Helicobacter pylori to several drugs reflects a worldwide problem, and it has been the intention of numerous researchers to overcome this problem. Thus, in this study, Acacia nilotica fruits were subjected to HPLC analysis to detect their phenolic compounds and flavonoids. Moreover, A. nilotica‘s anti-H. pylori activity and its inhibitory activity against human hepatocellular carcinoma (HepG-2 cells) were reported. Various compounds with different concentrations, such as ferulic acid (5451.04 µg/mL), chlorogenic acid (4572.26 µg/mL), quercetin (3733.37 µg/mL), rutin (2393.13 µg/mL), gallic acid (2116.77 µg/mL), cinnamic acid (69.72 µg/mL), hesperetin (121.39 µg/mL) and methyl gallate (140.45 µg/mL), were detected. Strong anti-H. pylori activity at 31 mm was reported, compared to the positive control of the 21.67 mm inhibition zone. Moreover, the MIC and MBC were 7.8 µg/mL and 15.62 µg/mL, respectively, while the MIC and MBC of the positive control were 31.25 µg/mL. The concentration of MBC at 25%, 50% and 75% reflected H. pylori’s anti-biofilm activity of 70.38%, 82.29% and 94.22%, respectively. Good antioxidant properties of the A. nilotica flower extract were documented at 15.63, 62.50, 250 and 1000 µg/mL, causing the DPPH scavenging percentages of 42.3%, 52.6%, 65.5% and 80.6%, respectively, with a IC50 of 36.74 µg/mL. HepG-2 cell proliferation was inhibited (91.26%) using 500 µg/mL of flower extract with an IC50 of 176.15 µg/mL, compared to an IC50 of 395.30 µg/mL used against human normal melanocytes. Molecular docking was applied to investigate ferulic acid with the H. pylori (4HI0) crystal structure to determine the best binding mode that interacted most energetically with the binding sites. Molecular docking indicated that ferulic acid was a proper inhibitor for the 4HI0 protein enzyme of H. pylori. A low energy score (−5.58 Kcal/mol) was recorded as a result of the interaction of ferulic acid with the residue’s SER 139 active site caused by the O 29 atom, which was important for its antibacterial activity.
Identification of Aspergillus niger Aquaporins Involved in Hydrogen Peroxide Signaling
Authors: Laothanachareon Thanaporn , Asin-Garcia Enrique , Volkers Rita J. M. , Tamayo-Ramos Juan Antonio , Martins dos Santos Vitor A. P. , Schaap Peter J.
Published: April 2023

Aspergillus niger is a robust microbial cell factory for organic acid production. However, the regulation of many industrially important pathways is still poorly understood. The regulation of the glucose oxidase (Gox) expression system, involved in the biosynthesis of gluconic acid, has recently been uncovered. The results of that study show hydrogen peroxide, a by-product of the extracellular conversion of glucose to gluconate, has a pivotal role as a signaling molecule in the induction of this system. In this study, the facilitated diffusion of hydrogen peroxide via aquaporin water channels (AQPs) was studied. AQPs are transmembrane proteins of the major intrinsic proteins (MIPs) superfamily. In addition to water and glycerol, they may also transport small solutes such as hydrogen peroxide. The genome sequence of A. niger N402 was screened for putative AQPs. Seven AQPs were found and could be classified into three main groups. One protein (AQPA) belonged to orthodox AQP, three (AQPB, AQPD, and AQPE) were grouped in aquaglyceroporins (AQGP), two (AQPC and AQPF) were in X-intrinsic proteins (XIPs), and the other (AQPG) could not be classified. Their ability to facilitate diffusion of hydrogen peroxide was identified using yeast phenotypic growth assays and by studying AQP gene knock-outs in A. niger. The X-intrinsic protein AQPF appears to play roles in facilitating hydrogen peroxide transport across the cellular membrane in both Saccharomyces cerevisiae and A. niger experiments.
Radiation Exposure and Frequency of Dental, Bitewing and Occlusal Radiographs in Children and Adolescents
Authors: Schüler Ina Manuela , Hennig Christoph-Ludwig , Buschek Rika , Scherbaum Rebecca , Jacobs Collin , Scheithauer Marcel , Mentzel Hans-Joachim
Published: April 2023

Dental radiographs are valuable diagnostic aids for oral healthcare, but exposure to ionizing radiation carries health risks, especially in children due to their high radio-sensitivity. Valid reference values for intraoral radiographs in children and adolescents are still missing. This study aimed to investigate the radiation dose values and underlying justifications of dental, bitewing and occlusal X-rays in children and adolescents. Data from routinely executed intraoral radiographs between 2002 and 2020 with conventional and digital tube-heads were extracted from the Radiology Information System. The effective exposure was calculated from technical parameters and statistical tests performed. A total number of 4455 intraoral (3128 dental, 903 bitewing and 424 occlusal) radiographs were investigated. For dental and bitewing radiographs, the dose area product (DAP) was 2.57 cGy × cm2 and the effective dose (ED) 0.77 µSv. For occlusal radiographs, the DAP was 7.43 cGy × cm2 and the ED 2.22 µSv. Overall, 70.2% of all intraoral radiographs were dental, 20.3% bitewing and 9.5% occlusal radiographs. The most frequent indication for intraoral radiographs was trauma (28.7%), followed by caries (22.7%) and apical diagnostics (22.7%). Moreover, 59.7% of all intraoral radiographs were taken in boys, especially for trauma (66.5%) and endodontics (67.2%) (p ≤ 0.00). Girls were significantly more frequently X-rayed for caries diagnostics than boys (28.1% vs. 19.1%, p ≤ 0.00). The average ED of 0.77 µSv for intraoral dental and bitewing radiographs in this study was within the range of other reported values. The technical parameters of the X-ray devices were found at the lowest recommended levels to best limit the radiation exposure and to assure acceptable diagnostic efficacy. Intraoral radiographs were performed predominantly for trauma, caries and apical diagnostics—reflecting general recommendations for the use of X-rays in children. For improved quality assurance and radiation protection, further studies are necessary to determine the meaningful dose reference level (DRL) for children.
Utilization of Circulating Tumor Cells in the Management of Solid Tumors
Authors: Kurniali Peter C. , Storandt Michael H. , Jin Zhaohui
Published: April 2023

Circulating tumor cells (CTCs) are tumor cells shed from the primary tumor into circulation, with clusters of CTCs responsible for cancer metastases. CTC detection and isolation from the bloodstream are based on properties distinguishing CTCs from normal blood cells. Current CTC detection techniques can be divided into two main categories: label dependent, which depends upon antibodies that selectively bind cell surface antigens present on CTCs, or label-independent detection, which is detection based on the size, deformability, and biophysical properties of CTCs. CTCs may play significant roles in cancer screening, diagnosis, treatment navigation, including prognostication and precision medicine, and surveillance. In cancer screening, capturing and evaluating CTCs from peripheral blood could be a strategy to detect cancer at its earliest stage. Cancer diagnosis using liquid biopsy could also have tremendous benefits. Full utilization of CTCs in the clinical management of malignancies may be feasible in the near future; however, several challenges still exist. CTC assays currently lack adequate sensitivity, especially in early-stage solid malignancies, due to low numbers of detectable CTCs. As assays improve and more trials evaluate the clinical utility of CTC detection in guiding therapies, we anticipate increased use in cancer management.
Transcription Factor ATF3 Participates in DeltaNp63-Mediated Proliferation of Corneal Epithelial Cells
Authors: Hsueh Yi-Jen , Meir Yaa-Jyuhn James , Hsiao Hui-Yi , Cheng Chao-Min , Ma Hui-Kang David , Wu Wei-Chi , Chen Hung-Chi
Published: April 2023

Understanding the regulatory mechanisms underlying corneal epithelial cell (CEC) proliferation in vitro may provide the means to boost CEC production in cell therapy for ocular disorders. The transcription factor ΔNp63 plays a crucial role in the proliferation of CECs, but the underlying mechanisms is yet to be elucidated. TP63 and ΔNp63 are encoded by the TP63 gene via alternative promoters. We previously reported that both ΔNp63 and activating transcription factor (ATF3) are substantially expressed in cultured CECs, but the regulatory relationship between ΔNp63 and ATF3 is unknown. In the present study, we found that ΔNp63 increased ATF3 expression and ATF3 promoter activity in cultured CECs. The deletion of the p63 binding core site reduced ATF3 promoter activity. CECs overexpressing ATF3 exhibited significantly greater proliferation than control CECs. ATF3 knockdown suppressed the ΔNp63-induced increase in cell proliferation. Overexpression of ATF3 in CECs significantly elevated protein and mRNA levels of cyclin D. The protein levels of keratin 3/14, integrin β1, and involucrin did not differ between ATF3-overexpressing CECs, ATF3-downregulated CECs, and control cells. In conclusion, our results suggest that ΔNp63 increases CEC proliferation via the ΔNp63/ATF3/CDK pathway.
Contrast-Induced Acute Kidney Injury and Endothelial Dysfunction: The Role of Vascular and Biochemical Parameters
Authors: Perrotta Adolfo Marco , Gigante Antonietta , Rotondi Silverio , Menè Paolo , Notturni Adriano , Schiavetto Stefano , Tanzilli Gaetano , Pellicano Chiara , Guaglianone Giuseppe , Tinti Francesca , Palange Paolo , Mazzaferro Sandro , Cianci Rosario , Lai Silvia
Published: April 2023

Introduction: Contrast-induced acute kidney injury (CIAKI) is one of the main causes of acute renal failure in hospitalized patients, following the administration of iodinated contrast medium used for CT scans and angiographic procedures. CIAKI determines a high cardiovascular risk and appears to be one of the most feared complications of coronary angiography, causing a notable worsening of the prognosis with high morbidity and mortality. Aim: To evaluate a possible association between the renal resistive index (RRI) and the development of CIAKI, as well as an association with the main subclinical markers of atherosclerosis and the main cardiovascular risk factors. Materials and Methods: We enrolled 101 patients with an indication for coronary angiography. Patients underwent an assessment of renal function (serum nitrogen and basal creatinine, 48 and 72 h after administration of contrast medium), inflammation (C reactive protein (CRP), serum calcium and phosphorus, intact parathormone (iPTH), 25-hydroxyvitaminD (25-OH-VitD), serum uric acid (SUA), total cholesterol, serum triglycerides, serum glucose and insulin). All patients also carried out an evaluation of RRI, intima-media thickness (IMT), interventricular septum (IVS) and the ankle-brachial index (ABI). Results: 101 patients (68 male), with a mean age of 73.0 ± 15.0 years, were enrolled for the study; 35 are affected by type 2 diabetes mellitus. A total of 19 cases of CIAKI were reported (19%), while among diabetic patients we reported an incidence of 23% (8 patients). In our study, patients with CIAKI had significantly higher RRI (p < 0.001) and IMT (p < 0.001) with respect to the patients who did not develop CIAKI. Furthermore, patients with CIAKI had significantly higher CRP (p < 0.001) and SUA (p < 0.006). Conclusions: We showed a significant difference in RRI, IMT, SUA and CRP values between the population developing CIAKI and patients without CIAKI. This data appears relevant considering that RRI and IMT are low-cost, non-invasive and easily reproducible markers of endothelial dysfunction and atherosclerosis.
The Revived Interest in Ageusia Research during the COVID-19 Pandemic: A Bibliometric Analysis
Authors: Yeung Andy Wai Kan
Published: April 2023

The evolution of ageusia research literature has yet to be investigated. This bibliometric study analyzed the entire ageusia research literature indexed in Web of Science, to reveal its growth and the most productive entities in terms of authors, institutions, countries, journals, and journal categories. In addition, this study aimed to identify medical conditions (and their treatments) that were frequently associated with ageusia. On 7 March 2022, the Web of Science Core Collection database was accessed with the following search query: TS = (ageusia OR “taste loss” OR “loss of taste” OR “loss of gustat*” OR “gustatory loss”). The search identified publications mentioning these terms in their title, abstract, or keywords. No additional filters were placed on publication year, language, etc. The basic publication and citation counts were extracted from the in-built functions of the database. The complete record of the publications was exported into VOSviewer, a bibliometric software for visualizations. The search yielded 1170 publications. The cumulative publication and citation counts of the ageusia research sharply increased in 2020. The most productive author was Professor Thomas Hummel from Technische Universität Dresden. Ageusia research had heavy contributions from the United States, Italy, the United Kingdom, Germany, and India. The top 5 most productive journals mainly belonged to the otorhinolaryngology and medicine categories. The medical conditions frequently investigated in ageusia research included COVID-19, cancers (head and neck, and advanced basal cell), Guillain-Barré syndrome, neurodegenerative diseases, diabetes, and Sjogren’s syndrome. This study could act as a begvinner’s guide for (1) clinicians who are not familiar with ageusia so that they might better understand which scenarios they need to be more aware of since ageusia could be a co-morbidity of a patient’s underlying disease, and (2) for those who wish to search for relevant authors and journals for suitable publications related to the topic.
Left Ventricular Assist Device as a Destination Therapy: Current Situation and the Importance of Patient Selection
Authors: Melendo-Viu María , Dobarro David , Raposeiras Roubin Sergio , Llamas Pernas Carmen , Moliz Cordón Candela , Vazquez Lamas Miriam , Piñón Esteban Miguel , Varela Martínez Maria Ángela , Abu Assi Emad , Pita Romero Rafael , Legarra Calderón Juan José , Íñiguez Romo Andrés
Published: April 2023

Advanced heart failure is a growing problem for which the best treatment is cardiac transplantation. However, the shortage of donors’ hearts made left ventricular assist devices as destination therapy (DT-LVAD) a highly recommended alternative: they improved mid-term prognosis as well as patients’ quality of life. Current intracorporeal pumps with a centrifugal continuous flow evolved in the last few years. Since 2003, when first LVAD was approved for long-term support, smaller device sizes with better survival and hemocompatibility profile were reached. The most important difficulty lies in the moment of the implant. Recent indications range from INTERMACS class 2 to 4, with close monitoring in intermediate cases. Moreover, a large multiparametric study is needed for considering the candidacy: basal situation, with a special interest in frailty, comorbidities, including renal and hepatic dysfunction, and medical background, considering every prior cardiac condition, must be evaluated. In addition, some clinical risk scores can be helpful to measure the possibility of right heart failure or morbi-mortality. With this review, we sought to summarize all the device improvements, with their updated clinical results, as well as to focus on all the patient selection criteria.
Publisher’s Note—MDPI Acquires Limnological Review
Authors: Aloè Carla
Published: April 2023

Limnological Review [...]
Limnological Review: Opening a New Chapter
Authors: Rzymski Piotr , Klimaszyk Piotr , Marszelewski Włodzimierz
Published: April 2023

Limnology is the study of inland water ecosystems, which are major contributors to biospheric diversity, structure, and function, even though they represent a small fraction of surface waters [...]
Developing a Support Vector Regression (SVR) Model for Prediction of Main and Lateral Bending Angles in Laser Tube Bending Process
Authors: Safari Mehdi , Rabiee Amir Hossein , Joudaki Jalal
Published: April 2023

The laser tube bending process (LTBP) is a new and powerful manufacturing method for bending tubes more accurately and economically by eliminating the bending die. The irradiated laser beam creates a local plastic deformation area, and the bending of the tube occurs depending on the magnitude of the heat absorbed by the tube and its material characteristics. The main bending angle and lateral bending angle are the output variables of the LTBP. In this study, the output variables are predicted by support vector regression (SVR) modeling, which is an effective methodology in machine learning. The SVR input data is provided by performing 92 experimental tests determined by the design of the experimental techniques. The measurement results are divided into two sub-datasets: 70% for the training dataset, and 30% for the testing dataset. The inputs of the SVR model are process parameters, which can be listed as the laser power, laser beam diameter, scanning speed, irradiation length, irradiation scheme, and the number of irradiations. Two SVR models are developed for the prediction of the output variables separately. The SVR predictor achieved a mean absolute error of 0.021/0.003, a mean absolute percentage error of 1.485/1.849, a root mean square error of 0.039/0.005, and a determination factor of 93.5/90.8% for the main/lateral bending angle. Accordingly, the SVR models prove the possibility of applying SVR to the prediction of the main bending angle and lateral bending angle in LTBP with quite an acceptable accuracy.
Modeling of Reactive Sputtering—History and Development
Authors: Shapovalov Viktor I.
Published: April 2023

This work critically reviews the evolution of reactive sputtering modeling that has taken place over the last 50 years. The review summarizes the main features of the deposition of simple metal compound films (nitrides, oxides, oxynitrides, carbides, etc.) that were experimentally found by different researchers. The above features include significant non-linearity and hysteresis. At the beginning of the 1970s, specific chemisorption models were proposed. These models were based on the assumption that a compound film was formed on the target due to chemisorption. Their development led to the appearance of the general isothermal chemisorption model, which was supplemented by the processes on the surfaces of the vacuum chamber wall and the substrate. The model has undergone numerous transformations for application to various problems of reactive sputtering. At the next step in the development of modeling, the reactive sputtering deposition (RSD) model was proposed, which was based on the implantation of reactive gas molecules into the target, bulk chemical reaction, chemisorption, and the “knock-on effect”. Another direction of the modeling development is represented by the nonisothermal physicochemical model, in which the Langmuir isotherm and the law of mass action are used. Various modifications of this model allowed describing reactive sputtering processes in more complex cases when the sputtering unit included a hot target or a sandwich one.
pH-Effect in the Fabrication of ZnO Nanostructured Thin Films by Chemical Bath Deposition for Increasing the Efficiency of Solar Cells
Authors: Garcia-Barrientos Abel , Ambrosio-Lazaro Roberto Carlos , Ramirez-Bone Rafael , Garcia-Ramirez Mario A. , Perez-Cortes Obed , Tapia-Olvera Ruben , Plaza-Castillo Jairo
Published: April 2023

In this study, the impact of pH on the production of ZnO nanostructured thin films using chemical bath deposition was investigated for the purpose of enhancing the efficiency of solar cells. The ZnO films were directly deposited onto glass substrates at various pH levels during the synthesis process. The results indicate that the crystallinity and overall quality of the material were not affected by the pH solution, as observed through X-ray diffraction patterns. However, scanning electron microscopy revealed that surface morphology improved with increasing pH values, leading to changes in the size of the nanoflowers between pH 9 and 11 values. Furthermore, the ZnO nanostructured thin films synthesized at pH levels of 9, 10, and 11 were utilized in the fabrication of dye-sensitized solar cells. The ZnO films synthesized at pH 11 exhibited superior characteristics in short-circuit current density and open-circuit photo-voltage compared with those produced at lower pH values.
Glass-Ceramic Coating on Silver Electrode Surface via 3D Printing
Authors: Yang Lilin , Wang Dongzhi , Zhou Guoxiang , Lan Zhidan , Yang Zhihua
Published: April 2023

Silver electrodes are commonly used as a conductive layer for electromagnetic devices. It has the advantages of good conductivity, easy processing, and good bonding with a ceramic matrix. However, the low melting point (961 °C) results in a decrease in electrical conductivity and migration of silver ions under an electric field when it works at high temperatures. Using a dense coating layer on the silver surface is a feasible way to effectively prevent the performance fluctuation or failure of the electrodes without sacrificing its wave-transmitting performance. Calcium-magnesium-silicon glass-ceramic (CaMgSi2O6) is a diopside material that has been widely used in electronic packaging materials. However, CaMgSi2O6 glass-ceramics (CMS) are facing tough challenges, such as high sintering temperature and insufficient density after sintering, which significantly confine its applications. In this study, CaO, MgO, B2O3, and SiO2 were used as raw materials to manufacture a uniform glass coating on the silver and Al2O3 ceramics surface via 3D printing technology followed by high-temperature sintering. The dielectric and thermal properties of the glass/ceramic layer prepared with various CaO-MgO-B2O3-SiO2 components were studied, and the protective effect of the glass-ceramic coating on the silver substrate at high temperatures were evaluated. It was found that the viscosity of the paste and the surface density of the coating increase with the increase of solid contents. The 3D-printed coating shows well-bonded interfaces between the Ag layer, the CMS coating, and the Al2O3 substrate. The diffusion depth was 2.5 μm, and no obvious pores and cracks can be detected. According to the high density and well-bonded glass coating, the silver was well protected from the corrosion environment. Increasing the sintering temperature and extending the sintering time is beneficial to form the crystallinity and the densification effect. This study provides an effective method to manufacture a corrosive-resistant coating on an electrically conductive substrate with outstanding dielectric performances.
Steering Potential for Printing Highly Aligned Discontinuous Fibre Composite Filament
Authors: Krajangsawasdi Narongkorn , Nguyen Duc H. , Hamerton Ian , Woods Benjamin K. S. , Ivanov Dmitry S. , Longana Marco L.
Published: April 2023

DcAFF (discontinuous aligned fibre filament) is a novel material for fused filament fabrication (FFF) 3D printing made of highly aligned discontinuous fibres produced using high performance discontinuous fibre (HiPerDiF) technology. It reinforces a thermoplastic matrix to provide high mechanical performance and formability. Accurate printing of DcAFF poses a challenge, especially for complex geometries, because: (i) there is a discrepancy between the path where the filament experiences the adhering pressure from the filleted nozzle and the nozzle path; and (ii) the rasters display poor adhesion to the build platform immediately after deposition, which causes the filament to be dragged when the printing direction changes. This paper explains the implication of these phenomena on steering capabilities and examines the techniques for improving DcAFF printing accuracy. In the first approach, the machine parameters were adjusted to improve the quality of the sharp turning angle without changing the desired path, but this showed insignificant effects in terms of precision improvements. In the second approach, a printing path modification with a compensation algorithm was introduced. The nature of the inaccuracy of the printing at the turning point was studied with a first-order lag relationship. Then the equation to describe the deposition raster inaccuracy was determined. A proportional–integral (PI) controller was added to the equation to calculate the nozzle movement in order to bring the raster back to the desired path. The applied compensation path is shown to give an accuracy improvement in curvilinear printing paths. This is particularly beneficial when printing larger circular diameter curvilinear printed parts. The developed printing approach can be applied with other fibre reinforced filaments to achieve complex geometries.
MRI Quantitative Evaluation of Muscle Fatty Infiltration
Authors: Chianca Vito , Vincenzo Bottino , Cuocolo Renato , Zappia Marcello , Guarino Salvatore , Di Pietto Francesco , Del Grande Filippo
Published: April 2023

Magnetic resonance imaging (MRI) is the gold-standard technique for evaluating muscle fatty infiltration and muscle atrophy due to its high contrast resolution. It can differentiate muscular from adipose tissue accurately. MRI can also quantify the adipose content within muscle bellies with several sequences such as T1-mapping, T2-mapping, spectroscopy, Dixon, intra-voxel incoherent motion, and diffusion tensor imaging. The main fields of interest in musculoskeletal radiology for a quantitative MRI evaluation of muscular fatty infiltration include neuro-muscular disorders such as myopathies, and dystrophies. Sarcopenia is another important field in which the evaluation of the degree of muscular fat infiltration or muscular hypotrophy is required for a correct diagnosis. This review highlights several MRI techniques and sequences focusing on quantitative methods of assessing adipose tissue and muscle atrophy.
Performance of TiO2-Based Tubular Membranes in the Photocatalytic Degradation of Organic Compounds
Authors: Barquín Carmen , Vital-Grappin Aranza , Kumakiri Izumi , Diban Nazely , Rivero Maria J. , Urtiaga Ane , Ortiz Inmaculada
Published: April 2023

This work presents the photocatalytic degradation of organic pollutants in water with TiO2 and TiO2/Ag membranes prepared by immobilising photocatalysts on ceramic porous tubular supports. The permeation capacity of TiO2 and TiO2/Ag membranes was checked before the photocatalytic application, showing high water fluxes (≈758 and 690 L m−2 h−1 bar−1, respectively) and <2% rejection against the model pollutants sodium dodecylbenzene sulfonate (DBS) and dichloroacetic acid (DCA). When the membranes were submerged in the aqueous solutions and irradiated with UV-A LEDs, the photocatalytic performance factors for the degradation of DCA were similar to those obtained with suspended TiO2 particles (1.1-fold and 1.2-fold increase, respectively). However, when the aqueous solution permeated through the pores of the photocatalytic membrane, the performance factors and kinetics were two-fold higher than for the submerged membranes, mostly due to the enhanced contact between the pollutants and the membranes photocatalytic sites where reactive species were generated. These results confirm the advantages of working in a flow-through mode with submerged photocatalytic membranes for the treatment of water polluted with persistent organic molecules, thanks to the reduction in the mass transfer limitations.
Hot Forging Die Design Optimization Using FEM Analysis for Near-Net Forming of 18CrNiMo7-6 Steel Pinion Shaft
Authors: Rajendran Nijenthan , Yurgel Charles Chemale , Misiolek Wojciech Z. , Alves de Sousa Ricardo
Published: April 2023

The objective of the presented work was to develop a new forging process for a pinion shaft as a component of a wind turbine. A study of near-net-shape forming using Deform 3D software was performed to reduce operational cost, time, and material scrap; enhance specific properties; increase productivity. Near-net forged products have good dimensional accuracy and continuous metal flow lines, which are characteristic of improved mechanical properties. To avoid the traditional trial-and-error experimental method, the process and tool design were accomplished with a careful and detailed numerical simulation approach. In the present work, the Finite Element Method was used to develop a process model for the existing hot forging process of the 18CrNiMo7-6 steel pinion shaft used in a wind turbine. The developed numerical process model was validated via experiment including a comparison of the metal flow lines from the FEM model with the metallography results of the forged part. Two new die designs were proposed, and the simulation results were compared to the actual process to achieve improved geometry. The results for the new geometries showed improvements in terms of the die cavity filling for the new proposed dies and better results in grain flow orientation. Compared to the initial non-optimized die, the new designs improved the mechanical properties and savings associated with the lower volume of required raw material and fewer finishing operations. Considering the applied stresses and wear in the new near-net shape, the die geometry shall be updated to accommodate more severe solicitations. Naturally, all the improvements carried out are dependent on other factors such as the conditions of the equipment, operator skills, lubrication, and other variables. A surface heat treatment is also suggested for stress relief as a reliability improvement.
Effect of Temperature on the Tribological Properties of Hafnium Carbonitrides Coatings
Authors: Aperador Willian , Bautista-Ruiz Jorge , Sánchez-Molina Jorge
Published: April 2023

For industrial processes in which refractory metals are necessary, hafnium carbonitride exhibits excellent performance due to its high thermal conductivity and resistance to oxidation. In this study, hafnium carbonitride was deposited on Inconel 718 steel and silicon (100) substrates. The objective was to characterize the wear properties as a function of temperature. The layers were deposited by physical vapor deposition (PVD) in an R.F. sputtering magnetron system from carbon targets and high-purity hafnium (99.99%). The wear tests were carried out at temperatures of 100 °C, 200 °C, 400 °C, and 800 °C in non-lubricated conditions. The coefficient of friction (COF) was recorded in situ. The heat treatment temperature on coatings is essential in determining anti-wear efficiency. It was determined that high temperatures (800 °C) improve resistance to wear. High-resolution XPS spectra were used to detect the chemical states of Hf 4f5/2 and Hf 4f7/2. The 4f5/2 and 4f7/2 binding energy indicates the presence of HfN and HfC. Using the TEM technique in bright field mode allowed us to know the orientation, crystallographic structure and interplanar distances of the HfCN. The topography of the coatings, by AFM, shows uniform grains and very small characteristics that determine the low surface roughness value. The SEM image of the cross-section of the HfCN coating shows homogeneity of the layer; no cracks or deformations are observed.
Study on the Preparation and Performance of Low-Temperature Sintering and High-Thermal-Conductivity Silver Nanowire Film
Authors: Peng Yongqian , Ye Yicong , Yu Cuijuan , Wang Zhen , Xu Yuanxi , Du Yongguo
Published: April 2023

This paper proposes a new silver nanoscale joining material, silver nanowire film, as an alternative joining approach for high-power and large-size chip packaging. The silver nanowire film was prepared by pressing filtration with silver nanowire that was synthesized using the polyol method. We found that the tensile strength of the film reached 3.40 MPa and the content of the silver reached up to 99.0 wt%. This paper further studies the influence of the size of silver nanowires on the performance of silver nanowire film. The experimental results show that the silver nanowire films prepared with silver nanowires with longer lengths and smaller diameters displayed better performances. The silver nanowire film with the best performance was prepared using silver nanowire with a diameter of 88 nm and a length of 29 μm. The thermal resistance of the sintered silver nanowire film that was hot-pressed at 250 °C 10 MPa was only 1.28 K∙W−1. The shear strength of the sintered joint was 56.4 MPa, and the fracture that occurred in the sintered silver nanowire film displayed a good plasticity.
Automatic Casting Control Method of Continuous Casting Based on Improved Soft Actor–Critic Algorithm
Authors: Wu Xiaojun , Jiang Wenze , Yuan Sheng , Kang Hongjia , Gao Qi , Mi Jinzhou
Published: April 2023

Continuous casting production is an important stage in smelting high-quality steel, and automatic casting control based on artificial intelligence is a key technology to improve the continuous casting process and the product quality. By controlling the opening degree of the stopper rod reasonably, the mold can be filled with liquid steel stably in the specified time window, and automatic casting can be realized. In this paper, an automatic casting control method of continuous casting based on an improved Soft Actor–Critic (SAC) algorithm is proposed. Firstly, a relational model of the stopper rod opening degree and the liquid steel outflow velocity is established according to historical casting data. Then the Markov Decision Process (MDP) model of the automatic casting problem and the reinforcement learning framework based on the SAC algorithm are established. Finally, a Heterogeneous Experience Pool (HEP) is introduced to improve the SAC algorithm. According to the simulation results, the proposed algorithm can predict the stopper rod opening degree sequence under the constraint of the target liquid level curve. Under different billet specifications and interference conditions, an accuracy of 80% of liquid level in the mold and a stopper rod opening degree stability rate of 75% can be achieved, which is 4.29% and 3.17% higher than those for the baseline algorithms, respectively.
A Lightweight Neural Network Based on GAF and ECA for Bearing Fault Diagnosis
Authors: Gu Xiaojiao , Xie Yuntao , Tian Yang , Liu Tianshun
Published: April 2023

A lightweight neural network fault diagnosis method based on Gramian angular field (GAF) feature map construction and efficient channel attention (ECA) optimization is presented herein to address the problem of the complex structure of traditional neural networks in bearing fault diagnosis. Firstly, a GAF is used to encode vibration signals into a temporal image. Secondly, the double-layer separation residual convolution neural network (DRCNN) is used to learn advanced features of the sample. The multi-branch structure is used as the receiving domain. ECA learns the correlation between feature channels. The extracted feature channels are adaptively weighted by adding a small additional computational cost. Finally, the method is tested and evaluated using wind turbine bearing data. The experimental results show that, compared with the traditional neural network, the DRCNN model based on GAF achieves higher diagnostic accuracy with less parameter calculation.
Thymoquinone Antifungal Activity against Candida glabrata Oral Isolates from Patients in Intensive Care Units—An In Vitro Study
Authors: Nouri Noura , Mohammadi Shahla Roudbar , Beardsley Justin , Aslani Peyman , Ghaffarifar Fatemeh , Roudbary Maryam , Rodrigues Célia Fortuna
Published: April 2023

The number of Candida spp. infections and drug resistance are dramatically increasing worldwide, particularly among immunosuppressed patients, and it is urgent to find novel compounds with antifungal activity. In this work, the antifungal and antibiofilm activity of thymoquinone (TQ), a key bioactive constituent of black cumin seed Nigella sativa L., was evaluated against Candida glabrata, a WHO ‘high-priority’ pathogen. Then, its effect on the expression of C. glabrata EPA6 and EPA7 genes (related to biofilm adhesion and development, respectively) were analyzed. Swab samples were taken from the oral cavity of 90 hospitalized patients in ICU wards, transferred to sterile falcon tubes, and cultured on Sabouraud Dextrose Agar (SDA) and Chromagar Candida for presumptive identification. Next, a 21-plex PCR was carried out for the confirmation of species level. C. glabrata isolates underwent antifungal drug susceptibility testing against fluconazole (FLZ), itraconazole (ITZ), amphotericin B (AMB), and TQ according to the CLSI microdilution method (M27, A3/S4). Biofilm formation was measured by an MTT assay. EPA6 and EPA7 gene expression was assessed by real-time PCR. From the 90 swab samples, 40 isolates were identified as C. glabrata with the 21-plex PCR. Most isolates were resistant to FLZ (n = 29, 72.5%), whereas 12.5% and 5% were ITZ and AMB resistant, respectively. The minimum inhibitory concentration (MIC50) of TQ against C. glabrata was 50 µg/mL. Importantly, TQ significantly inhibited the biofilm formation of C. glabrata isolates, and EPA6 gene expression was reduced significantly at MIC50 concentration of TQ. TQ seems to have some antifungal, antibiofilm (adhesion) effect on C. glabrata isolates, showing that this plant secondary metabolite is a promising agent to overcome Candida infections, especially oral candidiasis.
Hierarchical Design of CuO/Nickel–Cobalt–Sulfide Electrode by a Facile Two-Step Potentiostatic Deposition
Authors: Lv Sa , Geng Peiyu , Chi Yaodan , Wang Huan , Chu Xuefeng , Zhao Yang , Wu Boqi , Shang Wenshi , Wang Chao , Yang Jia , Cheng Zhifei , Yang Xiaotian
Published: April 2023

Herein, a scalable electrodeposition strategy is proposed to achieve hierarchical CuO/nickel–cobalt–sulfide (NCS) electrodes using two-step potentiostatic deposition followed by high-temperature calcination. The introduction of CuO provides support for the further deposition of NSC to ensure a high load of active electrode materials, thus generating more abundant active electrochemical sites. Meanwhile, dense deposited NSC nanosheets are connected to each other to form many chambers. Such a hierarchical electrode prompts a smooth and orderly transmission channel for electron transport, and reserves space for possible volume expansion during the electrochemical test process. As a result, the CuO/NCS electrode exhibits superior specific capacitance (Cs) of 4.26 F cm−2 at 20 mA cm−2 and remarkable coulombic efficiency of 96.37%. Furthermore, the cycle stability of the CuO/NCS electrode remains at 83.05% within 5000 cycles. The multistep electrodeposition strategy provides a basis and reference for the rational design of hierarchical electrodes to be applied in the field of energy storage.
Design and Development of a Traveling Wave Ferro-Microfluidic Device and System Rig for Potential Magnetophoretic Cell Separation and Sorting in a Water-Based Ferrofluid
Authors: Hewlin Rodward L. , Edwards Maegan , Schultz Christopher
Published: April 2023

The timely detection and diagnosis of diseases and accurate monitoring of specific genetic conditions require rapid and accurate separation, sorting, and direction of target cell types toward a sensor device surface. In that regard, cellular manipulation, separation, and sorting are progressively finding application potential within various bioassay applications such as medical disease diagnosis, pathogen detection, and medical testing. The aim of this paper is to present the design and development of a simple traveling wave ferro-microfluidic device and system rig purposed for the potential manipulation and magnetophoretic separation of cells in water-based ferrofluids. This paper details in full: (1) a method for tailoring cobalt ferrite nanoparticles for specific diameter size ranges (10–20 nm), (2) the development of a ferro-microfluidic device for potentially separating cells and magnetic nanoparticles, (3) the development of a water-based ferrofluid with magnetic nanoparticles and non-magnetic microparticles, and (4) the design and development of a system rig for producing the electric field within the ferro-microfluidic channel device for magnetizing and manipulating nonmagnetic particles in the ferro-microfluidic channel. The results reported in this work demonstrate a proof of concept for magnetophoretic manipulation and separation of magnetic and non-magnetic particles in a simple ferro-microfluidic device. This work is a design and proof-of-concept study. The design reported in this model is an improvement over existing magnetic excitation microfluidic system designs in that heat is efficiently removed from the circuit board to allow a range of input currents and frequencies to manipulate non-magnetic particles. Although this work did not analyze the separation of cells from magnetic particles, the results demonstrate that non-magnetic (surrogates for cellular materials) and magnetic entities can be separated and, in some cases, continuously pushed through the channel based on amperage, size, frequency, and electrode spacing. The results reported in this work establish that the developed ferro-microfluidic device may potentially be used as an effective platform for microparticle and cellular manipulation and sorting.
SiamHAS: Siamese Tracker with Hierarchical Attention Strategy for Aerial Tracking
Authors: Liu Faxue , Liu Jinghong , Chen Qiqi , Wang Xuan , Liu Chenglong
Published: April 2023

For the Siamese network-based trackers utilizing modern deep feature extraction networks without taking full advantage of the different levels of features, tracking drift is prone to occur in aerial scenarios, such as target occlusion, scale variation, and low-resolution target tracking. Additionally, the accuracy is low in challenging scenarios of visual tracking, which is due to the imperfect utilization of features. To improve the performance of the existing Siamese tracker in the above-mentioned challenging scenes, we propose a Siamese tracker based on Transformer multi-level feature enhancement with a hierarchical attention strategy. The saliency of the extracted features is enhanced by the process of Transformer Multi-level Enhancement; the application of the hierarchical attention strategy makes the tracker adaptively notice the target region information and improve the tracking performance in challenging aerial scenarios. Meanwhile, we conducted extensive experiments and qualitative or quantitative discussions on UVA123, UAV20L, and OTB100 datasets. Finally, the experimental results show that our SiamHAS performs favorably against several state-of-the-art trackers in these challenging scenarios.
Development and Research of the Sensitive Element of the MEMS Gyroscope Manufactured Using SOI Technology
Authors: Naumenko Danil , Tkachenko Alexey , Lysenko Igor , Kovalev Andrey
Published: April 2023

In this article, based on the developed methodology, the stages of designing the sensitive element of a microelectromechanical gyroscope with an open-loop structure are considered. This structure is intended for use in control units for mobile objects such as robots, mobile trolleys, etc. To quickly obtain a ready-made gyroscope, a specialized integrated circuit (SW6111) was selected, for the use of which the electronic part of the sensitive element of the microelectromechanical gyroscope was developed. The mechanical structure was also taken from a simple design. The simulation of the mathematical model was carried out in the MATLAB/Simulink software environment. The mechanical elements and the entire structure were calculated using finite element modeling with ANSYS MultiPhysics CAD tools. The developed sensitive element of the micromechanical gyroscope was manufactured using bulk micromachining technology−silicon-on-insulator−with a structural layer thickness equal to 50 μm. Experimental studies were carried out using a scanning electron microscope and a contact profilometer. Dynamic characteristics were measured using a Polytec MSA-500 microsystem analyzer. The manufactured structure has low topological deviations. Calculations and experiments showed fairly accurate results for the dynamic characteristics, with an error of less than 3% for the first iteration of the design.
Palaeoweathering Conditions, Provenance, and Tectonic Setting of the Early Silurian Longmaxi Formation in the Upper Yangtze Region (Southern China): Evidence from Geochemistry
Authors: Zhao Liang , Li Yong , Zou Chengjie , Zhao Shaoze , Wu Chaorong
Published: April 2023

The Longmaxi-1 black shales in the Upper Yangtze region are a vital source rocks in southern China. This study investigates the palaeoweathering conditions, provenance, and tectonic setting of the Longmaxi-1 black shale from an elemental geochemical perspective, ultimately revealing its tectonic setting. The results showed that the Longmaxi-1 black shales in the sedimentary period have the characteristics of primary deposition. The Longmaxi-1 black shales were deposited as a primary deposition under a mainly humid climate. However, fluctuations in climatic conditions were recorded from the bottom to the upper part of the formation. The parent rock of the Longmaxi-1 black shales in the Upper Yangtze region is a mixture provenance, mainly composed of intermediate-acid volcanic lithologies (granite and granodiorite), followed by mature quartzite and basalt. Black shale deposition is related to the tectonic setting of active continental margin and island arc-continent collision. The Cathayian orogenic belts and the North Qiangling orogenic belt may have played a role in the genesis of the Longmaxi-1 black shales within the Upper Yangtze region. This study provides significant clues regarding the reconstruction of the palaeoclimatic and palaeogeographical conditions of the Upper Yangtze region during the Early Silurian period.
Study on Continuously Weakening Mechanism of Heap Leaching Velocity of Weathered Rare Earth Ores with the Increase of Ore Burial Depth
Authors: Liu Defeng , Yan Wenxin , Zhang Zhenyue , Guo Wenda , Chi Ruan
Published: April 2023

The column leaching experiments, CT, numerical simulation and fractal theory were adopted to study the continuously weakening mechanism of the heap leaching velocity of weathered rare earth ores with the increase of ore burial depth. The variation characteristics of pore structure and the seepage law of solution in ore samples at different depths during leaching were explored, respectively. The results showed that the variation of characteristic parameters of the pore structure in the lower ore samples was the main reason for the decrease in the leaching velocity. For the lower ore samples after leaching, the number of pores with a pore volume of less than 0.01 mm3 and a pore radius of less than 0.8 mm increased, while pore connectivity, the fractal dimension of the pore shape, the ratio of pore length to width and pore throat length reduced. The solution paths of the upper and middle ore samples were concentrated while that of the lower samples were few, even interrupted. The pore pressure of the ore samples decreased after leaching, especially that of the lower ore samples. The research results are helpful to enrich the basic theory on the heap leaching of weathered rare earth ores.