bluuebunny/crossref_metadata_2025 huggingface dataset:
Plant Community Composition and Carbon Stocks of a Community Reserve Forest in North-East India
Authors: Ao Aosanen , Changkija Sapu , Brearley Francis Q. , Tripathi Shri Kant
Published: January 2023

Anthropogenic activities are altering the structure and functioning of forests and their services to society. However, we know little about the degree to which such activities are changing the health of forests through edge effects in fragmented forests in different regions of the world. The present study was carried out in Minkong Community Reserve Forest of Nagaland (North-east India) with the aim to determine the effects of anthropogenic activities on floristic composition and diversity, population structure, and biomass and carbon (C) stocks in the core zone (CZ) and buffer zone (BZ) of the forest. We established 15 plots of 0.04 ha each in the two forest zones. We identified 31 trees, 18 shrubs, and 22 herbs in the CZ, and 22 trees, 25 shrubs, and 24 herbs in the BZ; tree species diversity was greater in the CZ whereas the diversity of shrubs and herbs was greater in the BZ. The values for tree density and basal area in the CZ and BZ were 303 and 197 individuals ha−1 and 32.6 and 22.2 m2 ha−1, respectively; in contrast, the shrub and herb density increased in the BZ (4470 and 50,200 individuals ha−1) compared to that of the CZ (2530 and 35,500 individuals ha−1). The total stand biomass (including that below-ground) was 327 Mg ha−1 in the CZ and 224 Mg ha−1 in the BZ. Similarly, the total ecosystem C stocks in the CZ and BZ were 224 Mg C ha−1 and 173 Mg C ha−1, indicating that the overall ecosystem C pool including soil in the CZ was approximately 30% greater than the BZ. These results show how fragmentation and anthropogenic disturbance can reduce forest diversity and C stocks and that community forest management can play a role in conserving biodiversity and act as an ecosystem management tool to mitigate climate change.
A Chemical Explanation for Variations in Antioxidant Capacity across Camellia sinensis L. Cultivars
Authors: Li Zhengzhen , Wu Yaxian , Zhang Lan , Hasan Md. Kamrul , Zhang Liping , Yan Peng , Fu Jianyu , Han Wenyan , Li Xin
Published: January 2023

Flavanols are known as the most important antioxidants in tea (Camellia sinensis), but their contribution to the antioxidant capacity across tea cultivars has not been quantified. This study explored whether the variations of antioxidant capacity across tea cultivars could be linked to variations in main flavanol concentrations using 20 widely planted Chinese tea cultivars. The results showed that concentrations of flavanols, both monomeric (total catechins; 3.77%–8.85% d.w.) and polymeric forms (condensed tannins; 9.48%–17.67% d.w.), varied largely across tea cultivars. The contribution of total catechins to the antioxidant capacity in tea (R2 = 0.54–0.55) was greater than that of condensed tannins (R2 = 0.35–0.36) and total phenolic concentrations (R2 = 0.33–0.36). Individual catechin components collectively explained 54.94%–56.03% of the variations in antioxidant capacity across tea cultivars. Epigallocatechin gallate (EGCG) was the leading catechin component that determined the antioxidant capacity in tea (p < 0.001), accounting for up to 57% of the differences in catechin composition between tea cultivars with high and low antioxidant capacities. These results suggested that flavanols were stronger predictors of the antioxidant capacity across tea cultivars compared to total phenolic concentrations, providing guidance for breeding tea cultivars with strong antioxidant capacities.
The Influence of Nano-CaCO3 on the Mechanical and Dynamic Thermo-Mechanical Properties of Core–Shell Structured Wood Plastic Composites
Authors: Xian Yu , Feng Taigang , Li Hongbo , Xing Zebing , Wang Ge , Cheng Haitao
Published: January 2023

Using bamboo residue and high-density polyethylene (HDPE) as the main raw materials, core–shell structured wood plastic composites (WPCs) with nano-CaCO3 filling shell were prepared by coextruded technology. The effect of nano-CaCO3 contents on the flexural properties, impact strength and dynamic thermo-mechanical properties of the core–shell structured WPCs were investigated. And the value of core–shell interface interaction parameter was calculated by dynamic thermodynamic parameters. The results showed that the flexural strength increased by 48.1% and 40.3% when it was loaded with 10%–15% nano-CaCO3, compared to the HDPE shell composites, respectively. While nano-CaCO3-filled HDPE shell had lower impact strength in comparison with HDPE shell, the field emission scanning electron microscope revealed proper interfacial adhesion between the core and shell layers. The storage modulus of core–shell structured WPCs increased as nano-CaCO3 contents increased, but the values were lower than that of the HDPE shell. Addition of nano-CaCO3 caused an increase in the glass transition temperature (Tg) of the core–shell structured WPCs, loss factor (tanδ) of the core–shell structured WPCs decreased. The value of the core–shell interface interaction parameter of the core–shell structured WPCs increased significantly after the nano-CaCO3 loading reached 15%, which was consistent with the bending test results. These findings demonstrate that the core–shell structured WPCs have excellent properties filling with nano-CaCO3 in the shell layer, and bamboo residue is added to the core layer.
Remote Sensing Assessment and Modeling of the Spatial Dynamics of Tree Stand Disturbance after the Impact of Siberian Silk Moth (Dendrolimus sibiricus)
Authors: Ponomarev Evgenii I. , Shvetsov Evgeny G. , Yakimov Nikita D. , Tretyakov Pavel D. , Goroshko Andrey A. , Sultson Svetlana M. , Mikhaylov Pavel V.
Published: January 2023

In this study, we have analyzed tree stand disturbance by hthe Siberian Silk Moth (Dendrolimus sibiricus Tschetverikov (Lepidoptera: Lasiocampidae)) in Central Siberia (Krasnoyarsk region, Russia) in 2015–2020. We considered two plots that experienced silk moth outbreaks in 2015–2018 and 2018–2020 and used satellite data (Terra/MODIS, Landsat/ETM/OLI), field forest inventory data, a meteorological data set, and a vegetation cover vector layer. Silk moth-disturbed areas were classified using NDVI, which was calculated for each 15-day period during the growing season (April–September). We obtained formalized descriptions of the temporal dynamics of the disturbed area. Next, we classified the degree of disturbance of the forest stand after the impact of the silk moth by the threshold method according to the ranges of NDVI anomalies. Based on the generalized data from the forest inventory, we performed a correlation analysis of the relationship between the main characteristics of forests and the classes of disturbance. Finally, using a series of regression equations, we described a procedure for predicting the degree of impact on the stand during the time of silk moth outbreaks in the dark-needle coniferous stands of Central Siberia.
Tree Growth and Vegetation Diversity in Northern Idaho Forest Water Reclamation Facilities
Authors: Joshi Eureka , Coleman Mark D.
Published: January 2023

Forest water reclamation can improve tree growth and renovate municipal wastewater. Although there are indications that long-term application may exceed forest assimilation capacity, there is limited information on the long-term effects of reclaimed water application on coniferous ecosystems. The purpose of our study was to assess the impacts of prolonged reclaimed water application on forest growth responses and vegetation diversity. We examined the effects of reclaimed water at five water reuse facilities established between 1978 and 2013 in a four-decade time series. We collected tree cores and stem measurements to determine current and retrospective increments. We assessed plant diversity with vegetation surveys. The greatest diameter response observed for reclaimed water amendment compared to controls was 166.1% for western redcedar, while Douglas-fir increased up to 116.4% and ponderosa pine increased up to 100.6%. The minimum response observed was 30.3%. Current annual increments showed that the basal area and volume were significantly greater at long-established facilities for reclaimed-water-amended plots. The understory vegetation diversity declined with application time, while overstory vegetation diversity increased with application time. We conclude that reclaimed water can be a valuable resource to improve forest productivity, but continued application without stocking control may have detrimental effects on forest growth and vegetation diversity.
Lactic Fermentation of Broccoli (Brassica oleracea var. italica) to Enhance the Antioxidant and Antiproliferative Activities
Authors: Iga-Buitrón Daniela , Torres-Maravilla Edgar , Bermúdez-Humaran Luis G. , Ascacio-Valdes Juan A. , Rodríguez-Herrera Raúl , Aguilar Cristóbal N. , Flores-Gallegos Adriana C.
Published: January 2023

Lactic acid bacteria (LAB) have been used for centuries to produce fermented foods. Cruciferous vegetables contain large amounts of health-promoting compounds such as glucosinolates (GLSs) and phenolics. GLSs and phenolics have been linked to antioxidant, anticancer, and immunosuppressive effects. However, it has been reported that some LAB strains are able to metabolize and enhance the activities and amounts of biomolecules through decarboxylation and/or reduction activities, with positive impacts on human diet and colorectal cancer (CRC) prevention. In the present work, the bioprocessing of broccoli by lactic fermentation was evaluated to produce a functional food using both spontaneous and induced fermentation (Levilactobacillus brevis and Lactococcus lactis as starter co-culture). Changes in the proximal composition, GLSs, and phenolic content as well as the antioxidant, antiproliferative, and immunosuppressive effect of the fermented product were evaluated in in vitro cellular models to validate their potential in CRC chemoprevention. The results demonstrated that fermented broccoli extracts increased the antioxidant activity in Caco2 cells and inhibited the proliferation of HT29 and HT116 cell lines in a concentration-dependent manner, with the best results on day 6 at a concentration of 600 µg/mL. Our findings also provide evidence that fermented broccoli could have an anti-inflammatory effect.
Use of Brewers’ Spent Grains as a Potential Functional Ingredient for the Production of Traditional Herzegovinian Product Ćupter
Authors: Lalić Anita , Karlović Andrea , Marić Marina
Published: January 2023

Ćupter is Herzegovinian candy made of must and flour/semolina. Much research about the incorporation of brewers’ spent grains into the human diet has been published. The purpose of this study was to partially replace semolina (Samples 1 and 2) and flour (Samples 3 and 4) with brewers’ spent grains originating from industrial (Samples 1 and 4) and craft breweries (Samples 2 and 3) and study nutritive, chemical, and preference properties of the product. In this research, the authors aimed to find application of this already proven functional ingredient in ćupter production. Values for pH were higher for all samples compared to the traditional recipe. Samples produced with flour had higher values of water activity (0.86 ± 0.01) and moisture (41.82 ± 1.68 and 41.11 ± 1.41). Ash content increased with BSG addition, but between samples, there were no significant differences. Collected data showed significant differences in fat levels. Higher protein content was measured for Samples 4 (6.60 ± 0.17) and 1 (6.13 ± 0.07). The highest total sugar content was measured for Sample 1. The general appearance for all samples was “moderately like”. Nutritive value was improved with the addition of BSG, but recipes and drying should be modified to improve consumer acceptance.
Screening of Antioxidant Effect of Spontaneous and Bioinoculated with Gluconobacter oxydans Fermented Papaya: A Comparative Study
Authors: Leitão Mariana , Ferreira Beatriz , Guedes Beatriz , Moreira Daniela , García Pablo A. , Barreiros Luisa , Correia Patrícia
Published: January 2023

Fermented papaya is recognized as a nutraceutical with a diverse and rich composition. Fermentation of fruit with desirable microorganisms could be a strategy to improve the nutritional quality and profile of the fruit. Despite the popularity of fermented papaya, there is still a lack of knowledge on the effects of various fermentation parameters. The goal of this study was to screen the antioxidant and other properties of the products obtained through a variety of fermentation experiments, as well as the impact of adding Gluconobacter oxydans on their physicochemical properties. The strategies used to produce the fermented papaya extracts were spontaneous fermentation and bioinoculation with G. oxydans. Different fermentation tests were performed to measure pH, total soluble solids, reducing sugars, sodium pyruvate content, total phenolic content (TPC), and ferric reducing antioxidant power (FRAP). There was a decrease in TPC during spontaneous fermentations (five assays). However, it can be observed that in the fermentation assays with G. oxydans, there was an increase in TPC and antioxidant properties. The highest content of TPC was observed on the eighth day of P7 (260.18 ± 0.02 µg gallic acid equivalents mL−1) which was fermented with the bacteria and supplemented with glucose. Therefore, phenolic compounds in fermented papaya were found to increase antioxidant capacity as a result of bioinoculation with G. oxydans.
Survey on Phthalates in Beer Packaged in Aluminum Cans, PET and Glass Bottles
Authors: Habschied Kristina , Kartalović Brankica , Lazić Dušan , Krstanović Vinko , Mastanjević Krešimir
Published: January 2023

Phthalates are known as endocrine disruptors and are common in plastic polymers, varnishes, and printing inks. However, they most often enter the human body through food. Plastic materials that hold food contain different chemicals, and phthalates are one of them. Phthalates can also be found in microplastics since microplastic particles serve as a vector for different chemicals that can be slowly released into food and beverages. The aim of this preliminary study was to determine the concentration and types of phthalates (dimethyl phthalate, diethyl phthalate, diisobutyl phthalate, dibutyl phthalate, bis (2-ethylhexyl) phthalate, di-n-octyl-phthalate) in beer packaged in aluminum cans, PET, and glass bottles. Ten aluminum-canned beers, sixteen PET-packaged, and eighteen glass-bottled beers were bought at a local food store and subjected to GC–MS analysis to quantify and qualify phthalates. The results indicate that PET-packaged beers can contain significant amounts of phthalates; in sample P10, the total sum of phthalates reached 219.82 µg/L. Especially high concentrations of dibutyl phthalate were found in all samples, but the highest concentration was detected in sample P13 at 92.17 µg/L. However, canned beers showed even higher levels of certain phthalates, such as bis (2-ethylhexyl) phthalate, which amounted to 326.81 µg/L in sample C1. In short, phthalates pose a serious health-concerning problem and should be regarded as such.
Unlocking the Potential of High-Amylose Starch for Gut Health: Not All Function the Same
Authors: Li Hai-Teng , Zhang Wenyu , Zhu Huijuan , Chao Chen , Guo Qing
Published: January 2023

High-amylose starch has unique functional properties and nutritional values in food applications. This type of starch is generally resistant to enzymatic digestion in the gastrointestinal tract, and contains an increased fraction of resistant starch (RS), which is a type of dietary fiber. The digestion and fermentation of high-amylose starch in the gut are of current research interest, as the processes are related to its nutritional functionality. This review summarizes recent in vitro and in vivo studies on the digestion and fermentation of high-amylose starches from different botanical sources and those that have been obtained by modifications. The RS content and fermentation properties are compared among high-amylose starches. This review aims to provide a current understanding of the relationship between high-amylose starch structures and fermentation-related nutritional properties. The results of these studies suggest that both modifications and food processing of high-amylose starch result in distinct fermentation products and nutritional properties. The review provides insight into the potential future applications of diverse high-amylose starches as bioactive compounds to modulate colonic fermentation.
Valorization of Fruit Waste for Bioactive Compounds and Their Applications in the Food Industry
Authors: Nirmal Nilesh , Khanashyam Anandu , Mundanat Anjaly , Shah Kartik , Babu Karthik , Thorakkattu Priyamvada , Al-Asmari Fahad , Pandiselvam Ravi
Published: January 2023

The fruit production and processing sectors produce tremendous amounts of by-products and waste that cause significant economic losses and an undesirable impact on the environment. The effective utilization of these fruit wastes can help to reduce the carbon footprint and greenhouse gas emissions, thereby achieving sustainable development goals. These by-products contain a variety of bioactive compounds, such as dietary fiber, flavonoids, phenolic compounds, antioxidants, polysaccharides, and several other health-promoting nutrients and phytochemicals. These bioactive compounds can be extracted and used as value-added products in different industrial applications. The bioactive components extracted can be used in developing nutraceutical products, functional foods, or food additives. This review provides a comprehensive review of the recent developments in fruit waste valorization techniques and their application in food industries. The various extraction techniques, including conventional and emerging methods, have been discussed. The antioxidant and antimicrobial activities of the active compounds extracted and isolated from fruit waste have been described. The most important food industrial application of bioactive compounds extracted from fruit waste (FW) has been provided. Finally, challenges, future direction, and concluding remarks on the topic are summarized.
Capture of Fermentation Gas from Fermentation of Grape Must
Authors: Prusova Bozena , Humaj Jakub , Kulhankova Michaela , Kumsta Michal , Sochor Jiri , Baron Mojmir
Published: January 2023

During alcoholic fermentation, a considerable amount of carbon dioxide (CO2) is produced, and the stream of CO2 can strip aromatic substances from the fermenting must. Aroma losses during fermentation can be significant and may lead to a reduction in wine quality. This study is focused on new fermentation gas capture technology. In the experiment, gas was captured during the fermentation of sauvignon blanc must. The concentration of individual volatile compounds in the fermentation gas was determined using gas chromatography, and the highest values were achieved by isoamyl acetate, isoamyl alcohol and ethyl decanoate. Ethyl dodecanoate achieved the lowest values of the investigated volatile substances. For sensory assessment, quantitative descriptive analysis (QDA) compared water carbonated with fermentation gas and water carbonated with commercial carbon dioxide for food purposes. Based on the results of this study, it can be concluded that the captured gas containing positive aromatic substances is suitable for the production of carbonated drinks in the food industry.
Lotus Root Polysaccharide-Phenol Complexes: Interaction, Structure, Antioxidant, and Anti-Inflammatory Activities
Authors: Peng Kaidi , Li Yin , Sun Ying , Xu Wei , Wang Hongxun , Zhang Rui , Yi Yang
Published: January 2023

This research aimed to explore the interaction between lotus root polysaccharides (LRPs) and phenolic compounds, and to study the effects of phenolic binding on the structural and functional properties of LRPs. The influences of pH, temperature, and NaCl and phenol concentration on the binding ratio of gallic acid (GA)/epigallocatechin (EGC) to LRPs were evaluated. LRP-GA/EGC complexes with different phenolic binding amounts were then prepared and characterized via ultraviolet–visible (UV–Vis) and Fourier-transform infrared (FTIR) spectroscopy, and average molecular weight (MW) measurements. The results suggest that hydrogen bonds contributed to the binding of GA/EGC and LRPs. The phenolic binding led to significant changes in the structure and MW of LRPs. Moreover, antioxidant activity and the macrophage-stimulating effect of LRPs were improved after binding with GA/EGC, depending on the binding amount and type of polyphenol. Interestingly, LRP-GA/EGC complexes with polyphenol binding amounts of 105.4 mg/g and 50.71 mg/g, respectively, showed better stimulation effects on the anti-inflammatory cytokine IL10 secretion of macrophages when compared to LRPs. These results show the great potential of phenolic binding to be applied to improve the structure and functional activity of LRPs.
A Novel Blockchain-Enabled Supply-Chain Management Framework for Xinjiang Jujube: Research on Optimized Blockchain Considering Private Transactions
Authors: Song Hao , Ge Wenfei , Gao Pan , Xu Wei
Published: January 2023

Excellent jujube supply-chain management is of great significance to the development of the Xinjiang jujube industry. However, traditional jujube supply-chain management is faced with the dilemmas of opaque connection of transaction flow and capital flow information, unreliable product traceability information, and jujube farmers’ lack of bargaining power. In this research, we propose a jujube supply-chain-management framework based on blockchain. Hyperledger fabric is the distributed solution platform of this research. The current blockchain-based traceability framework for agri-food emphasizes the construction process and ignores the performance and characteristics of the framework. This research optimizes the blockchain-network-topology architecture and storage cost of writing data. This not only solves the traceability of jujube, but also fills the gap in previous research on the traceability framework. Moreover, transactions are innovatively divided into common transactions and private transactions. Private transactions have enhanced the bargaining power of jujube farmers. Through the analysis of benchmark tests, the effectiveness and feasibility of the framework are verified.
A Review on Qualitative Assessment of Natural Gas Utilisation Options for Eliminating Routine Nigerian Gas Flaring
Authors: Abu Robin , Patchigolla Kumar , Simms Nigel
Published: January 2023

Natural gas flaring, with its harmful environmental, health, and economic effects, is common in the Nigerian oil and gas industry because of a lower tax regime for flared gases. Based on the adverse effects of flared gas, the Nigerian government has renewed and improved its efforts to reduce or eliminate gas flaring through the application of natural gas utilisation techniques. However, because the conventional approach to flare gas utilisation is heavily reliant on achieving scale, fuel, and end-product prices, not all technologies are technically and economically viable for typically capturing large and small quantities of associated gas from various flare sites or gas fields (located offshore or onshore). For these reasons, this paper reviews and compares various flare gas utilisation options to guide their proper selection for appropriate implementation in the eradication of routine gas flaring in Nigeria and to promote the Zero Routine Flaring initiative, which aims to reduce flaring levels dramatically by 2030. A qualitative assessment is used in this study to contrast the various flare gas utilisation options against key decision drivers. In this analysis, three natural gas utilisation processes—liquefied natural gas (LNG), gas to wire (GTW), and gas to methanol (GTM)—are recommended as options for Nigeria because of their economic significance, technological viability (both onshore and offshore), and environmental benefits. All these gas utilisation options have the potential to significantly reduce and prevent routine gas flaring in Nigeria and can be used separately or in combination to create synergies that could lower project costs and product market risk. This article clearly identifies the environmental benefits and the technical and economic viability of infrastructure investments to recover and repurpose flare gasses along with recommendation steps to select and optimise economies of scale for an associated natural gas utilisation option.
Antimicrobial Efficacy Assessment and Rheological Investigation of Two Different Hand Sanitizers Compared with the Standard Reference WHO Formulation 1
Authors: Sommatis Sabrina , Capillo Maria Chiara , Maccario Cristina , Rauso Raffaele , D’Este Edoardo , Herrera Martha , Castiglioni Mauro , Mocchi Roberto , Zerbinati Nicola
Published: January 2023

(1) Background: recently, the use of alcohol-based hand sanitizers (ABHSs) has become very frequent, and an evaluation of the stability and effectiveness of their formulations is a critical topic which should be carefully considered. (2) Methods: starting from the characterization of the hand sanitizers object of the study, our interest was focused on their rheological behavior in order to confirm their intrinsic features, but also the stability of each formulation in different conditions of shear and temperature; the second aspect concerns the antimicrobial assessment through a panel of in vitro and in vivo experimental trials. (3) Results: rheological investigation confirmed good stability for the two hand sanitizers in gel formula with respect to the reference in liquid formula; the antimicrobial activity evaluation showed good efficacy of each formulation both in vitro and in vivo. (4) Conclusions: altogether, our overview presents a valid quality control assessment to ensure the stability and efficacy of an alcohol-based hand sanitizer.
Cryostructuring of Polymeric Systems: 64. Preparation and Properties of Poly(vinyl alcohol)-Based Cryogels Loaded with Antimicrobial Drugs and Assessment of the Potential of Such Gel Materials to Perform as Gel Implants for the Treatment of Infected Wounds
Authors: Kolosova Olga Yu. , Shaikhaliev Astemir I. , Krasnov Mikhail S. , Bondar Ivan M. , Sidorskii Egor V. , Sorokina Elena V. , Lozinsky Vladimir I.
Published: January 2023

Physical macroporous poly(vinyl alcohol)-based cryogels formed by the freeze–thaw technique without the use of any foreign cross-linkers are of significant interests for biomedical applications. In the present study, such gel materials loaded with the antimicrobial substances were prepared and their physicochemical properties were evaluated followed by an assessment of their potential to serve as drug carriers that can be used as implants for the treatment of infected wounds. The antibiotic Ceftriaxone and the antimycotic Fluconazole were used as antimicrobial agents. It was shown that the Ceftriaxone additives caused the up-swelling effects with respect to the cryogel matrix and some decrease in its heat endurance but did not result in a substantial change in the gel strength. With that, the drug release from the cryogel vehicle occurred without any diffusion restrictions, which was demonstrated by both the spectrophotometric recording and the microbiological agar diffusion technique. In turn, the in vivo biotesting of such drug-loaded cryogels also showed that these materials were able to function as rather efficient antimicrobial implants injected in the artificially infected model wounds of laboratory rabbits. These results confirmed the promising biomedical potential of similar implants.
Identification and Characterization of Circular RNAs (circRNAs) Using RNA-Seq in Two Breeds of Cashmere Goats
Authors: Hu Liyan , Wang Jiqing , Luo Yuzhu , Liu Xiu , Li Shaobin , Hao Zhiyun , Zhao Fangfang , Li Mingna , Shi Bingang , Gu Yuanhua
Published: January 2023

Circular RNA (circRNA) is a type of non-coding RNA generated from back-splicing the reactions of linear RNA. It plays an important role in various cellular and biological processes. However, there are few studies about the regulatory effect of circRNAs on cashmere fiber traits in cashmere goats. In this study, the expression profiles of circRNAs in skin tissue were compared between Liaoning cashmere (LC) goats and Ziwuling black (ZB) goats, with a significant difference in cashmere fiber yield, cashmere fiber diameter, and cashmere fiber color, using RNA-seq. A total of 11,613 circRNAs were expressed in the caprine skin tissue, and their type, chromosomal distribution, and length distribution were characterized. A total of 115 up-regulated circRNAs and 146 down-regulated circRNAs in LC goats were screened compared to ZB goats. The authenticity of 10 differentially expressed circRNAs was validated by detecting their expression levels and the head-to-tail splice junction using RT-PCR and DNA sequencing, respectively. The parent genes of differentially expressed circRNA were mainly enriched in some Gene Ontology (GO) terms and pathways related to cashmere fiber traits, such as the canonical Wnt signaling pathway, which is involved in the regulation of cell promotion, stem cell proliferation, Wnt signaling pathway regulation, epithelial morphogenesis, MAPK signaling pathway, and cell adhesion molecules pathway. Eight differentially expressed circRNAs were further selected to construct a circRNA-miRNA network, and some miRNAs that were previously reported as related to fiber traits were found in the network. This study provides a deep understanding of the roles of circRNAs in the regulation of cashmere fiber traits in cashmere goats and the involvement of differential splicing in phenotypic expression according to breed and region.
Genome-Wide Identification, Evolutionary and Functional Analyses of WRKY Family Members in Ginkgo biloba
Authors: Li Weixing , Xiao Nan , Wang Yawen , Liu Ximeng , Chen Zhaoyu , Gu Xiaoyin , Chen Yadi
Published: January 2023

WRKY transcription factors (TFs) are one of the largest families in plants which play essential roles in plant growth and stress response. Ginkgo biloba is a living fossil that has remained essentially unchanged for more than 200 million years, and now has become widespread worldwide due to the medicinal active ingredients in its leaves. Here, 37 WRKY genes were identified, which were distributed randomly in nine chromosomes of G. biloba. Results of the phylogenetic analysis indicated that the GbWRKY could be divided into three groups. Furthermore, the expression patterns of GbWRKY genes were analyzed. Gene expression profiling and qRT−PCR revealed that different members of GbWRKY have different spatiotemporal expression patterns in different abiotic stresses. Most of the GbWRKY genes can respond to UV-B radiation, drought, high temperature and salt treatment. Meanwhile, all GbWRKY members performed phylogenetic tree analyses with the WRKY proteins of other species which were known to be associated with abiotic stress. The result suggested that GbWRKY may play a crucial role in regulating multiple stress tolerances. Additionally, GbWRKY13 and GbWRKY37 were all located in the nucleus, while GbWRKY15 was located in the nucleus and cytomembrane.
FRMD7 Gene Alterations in a Pakistani Family Associated with Congenital Idiopathic Nystagmus
Authors: Arshad Muhammad Waqar , Shabbir Muhammad Imran , Asif Saaim , Shahzad Mohsin , Leydier Larissa , Rai Sunil Kumar
Published: January 2023

Congenital idiopathic nystagmus (CIN) is an oculomotor disorder characterized by repetitive and rapid involuntary movement of the eye that usually develops in the first six months after birth. Unlike other forms of nystagmus, CIN is widely associated with mutations in the FRMD7 gene. This study involves the molecular genetic analysis of a consanguineous Pakistani family with individuals suffering from CIN to undermine any potential pathogenic mutations. Blood samples were taken from affected and normal individuals of the family. Genomic DNA was extracted using an in-organic method. Whole Exome Sequencing (WES) and analysis were performed to find any mutations in the causative gene. To validate the existence and co-segregation of the FRMD7 gene variant found using WES, sanger sequencing was also carried out using primers that targeted all of the FRMD7 coding exons. Additionally, the pathogenicity of the identified variant was assessed using different bioinformatic tools. The WES results identified a novel nonsense mutation in the FRMD7 (c.443T>A; p. Leu148 *) gene in affected individuals from the Pakistani family, with CIN resulting in a premature termination codon, further resulting in the formation of a destabilized protein structure that was incomplete. Co-segregation analysis revealed that affected males are hemizygous for the mutated allele c.443T>A; p. Leu148 * and the affected mother is heterozygous. Overall, such molecular genetic studies expand our current knowledge of the mutations associated with the FRMD7 gene in Pakistani families with CIN and significantly enhance our understanding of the molecular mechanisms involved in genetic disorders.
Informing Wildlife Corridor Creation through Population Genetics of an Arboreal Marsupial in a Fragmented Landscape
Authors: Gracanin Ana , Knipler Monica L. , Mikac Katarina M.
Published: January 2023

Habitat loss and fragmentation contribute significantly to the decline of arboreal mammal populations. As populations become fragmented and isolated, a reduction in gene flow can result in a loss of genetic diversity and have an overall impact upon long-term persistence. Creating wildlife corridors can mitigate such effects by increasing the movement and dispersal of animals, thus acting to reduce population isolation. To evaluate the success of a corridor, a before–after experimental research framework can be used. Here, we report the genetic diversity and structure of sugar glider (Petaurus breviceps) sampling locations within a fragmented landscape prior to the implementation of a wildlife corridor. This study used 5999 genome-wide SNPs from 94 sugar gliders caught from 8 locations in a fragmented landscape in south-eastern New South Wales, Australia. Overall genetic structure was limited, and gene flow was detected across the landscape. Our findings indicate that the study area contains one large population. A major highway dissecting the landscape did not act as a significant barrier to dispersal, though this may be because of its relatively new presence in the landscape (completed in 2018). Future studies may yet indicate its long-term impact as a barrier to gene flow. Future work should aim to repeat the methods of this study to examine the medium-to-long-term impacts of the wildlife corridor on sugar gliders, as well as examine the genetic structure of other native, specialist species in the landscape.
Variants Identified in the HOXC13 and HOXD13 Genes Suggest Association with Cervical Cancer in a Cohort of Mexican Women
Authors: Juárez-Rendón Karina Janett , Castro-García Manuel Alejandro , Prada-Ortega Diddier Giovanni , Rivera Gildardo , Ruíz-Godoy Luz María , Enríquez-Cárcamo Virginia Isabel , Reyes-Lopez Miguel Angel
Published: January 2023

HOX genes have been associated with carcinogenesis. However, the molecular mechanism by which tumors are generated remains unclear. The HOXC13 and HOXD13 genes are of interest for their involvement in the development of genitourinary structures. The aim of this first study in the Mexican population was to search for and analyze variants in the coding region of the HOXC13 and HOXD13 genes in women with cervical cancer. Samples from Mexican women with cervical cancer and healthy women were sequenced (50/50). Allelic and genotypic frequencies were compared between groups. The functional impact of the proteins was determined with two bioinformatics servers (SIFT and PolyPhen-2), and the oncogenic potential of the identified nonsynonymous variants was determined using the CGI server. We identified five unreported gene variants: c.895C>A p.(Leu299Ile) and c.777C>T p.(Arg259Arg) in the HOXC13 gene and c.128T>A p.(Phe43Tyr), c.204G>A p.(Ala68Ala), and c.267G>A p.(Ser89Ser) in the HOXD13 gene. In this study, we suggest that the non-synonymous variants c.895C>A p.(Leu299Ile) and c.128T>A p.(Phe43Tyr) could represent a risk factor for the development of the disease, although additional studies in larger patient populations and in different ethnic groups are needed in order to support the results observed.
Competition for Light Interception in Different Plant Canopy Characteristics of Diverse Cotton Cultivars
Authors: Sultana Fahmida , Dev Washu , Xin Minghua , Han Yingchun , Feng Lu , Lei Yaping , Yang Beifang , Wang Guoping , Li Xiaofei , Wang Zhanbiao , Xing Fangfang , Xiong Shiwu , Li Yabing
Published: January 2023

Identifying the ideal plant nature and canopy structure is of great importance for improving photosynthetic production and the potential action of plants. To address this challenge, an investigation was accomplished in 2018 and 2019 at the Institute of Cotton Research (ICR) of the Chinese Academy of Agricultural Science (CAAS), Henan Province, China. Six cotton varieties with diverse maturities and plant canopy structures were used to evaluate the light interception (LI) in cotton, the leaf area index (LAI), the biomass, and the yield throughout the two years of study. The light spatial distribution in the plant canopy was evaluated using a geographic statistical method, following the increasing quantity of radiation intercepted, which was determined using the rules of Simpson. Compared to the cotton plants with a compact structure, varieties with both a loose and tower design captured a comparatively higher amount of light (average 31.3%) and achieved a higher LAI (average 32.4%), eventually achieving a high yield (average 10.1%). Furthermore, the polynomial correlation revealed a positive relationship between the biomass accumulation in the reproductive parts and canopy-accrued light interception (LI), signifying that light interception is critical for the yield development of cotton. Furthermore, when the leaf area index (LAI) was peaked, radiation interception and biomass reached the highest during the boll-forming stage. These findings will provide guidance on the light distribution in cotton cultivars with an ideal plant structure for light capture development, providing an important foundation for researchers to better manage light and canopies.
The Bologna Interpretation of Rock Bridges
Authors: Elmo Davide
Published: January 2023

One can only know where a rock bridge is once one measures it. In addition, to measure it, you need the rock mass to fail. This critical problem is ignored by many, and engineers continue to refer to rock bridges as geometrical distances between non-persistent fractures. This paper argues that this rather simplistic approach can lead to non-realistic failure mechanisms. We also raise the critical question of whether the inappropriate functioning of strength equations centred on the measurement of rock bridge percentages could result in misinterpreting the risk of failure. We propose a new interpterion, aptly called the Bologna Interpretation, as an analogy to the Copenhagen Interpretation of quantum mechanics, to highlight the indeterministic nature of rock bridges and to honour the oldest university in Europe (Bologna University). The Bologna Interpretation does not negate the existence of rock bridges. What rock bridges look like, how many there are, and where they are, we do not know; we can assume their existence and account for their contribution to rock mass strength using a potential analogue.
Ostracod and Foraminifer Responses to Late Pleistocene–Holocene Volcanic Activity in Northern Victoria Land as Recorded in Ross Sea (Antarctica) Marine Sediments
Authors: Salvi Gianguido , Melis Romana , Del Carlo Paola , Di Roberto Alessio
Published: January 2023

The impacts on ostracods and foraminifers caused by three Late Quaternary ashfalls of different intensities and recovered in the ANTA02-NW2 core sediments (Drygalski Basin, western Ross Sea) were analysed for the first time. Albeit with different timing, both associations demonstrated similar response patterns associated with the deposition of material from volcanic eruptions. In particular, based on the palaeontological evidence, it was possible to divide the cores into four intervals/phases recording the evolution of the ecosystem before and after the deposition events: (1) Pre-extinction phase (high abundance and high diversity values). (2) Extinction phase, characterised by the complete disappearance of ostracod fauna; the foraminiferal assemblage, although not entirely absent, records extremely low values of abundance and diversity (survivor assemblage). (3) Recovery phase (increasing abundance and diversity values), characterised by the recolonisation of some opportunistic taxa; species such as Australicythere devexa and Australicythere polylyca dominate the ostracod assemblage. (4) Post-extinction phase (high abundance and high diversity values), with the return to an environmental equilibrium characterised by the colonisation of specialised taxa such as Argilloecia sp., Cytheropteron sp., Echinocythereis sp., and Hemicytherura spp. Our results may aid in the understanding of how communities (i.e., ostracods and foraminifers) recovered after the impact of direct deposits of volcanic ash into ocean waters. The mechanisms by which disappearance and/or mortality was induced are still not clear. The release of toxic metals during the reaction of the volcanic ash with seawater, the resulting chemical alteration in the seawater, and the change in pH, together with the possible suppression of planktonic organisms, may have caused the two main extinction phases recorded by the ANTA02-NW2 core sediments.
The Status of Geoethical Thinking in the Educational System of Greece: An Overview
Authors: Mosios Spyros , Georgousis Efthymios , Drinia Hara
Published: January 2023

In recent years, the concern about geoethics in Greece has begun to grow. This review aims to present the current geoethical thinking in Greece’s educational system through a thorough research of all educational levels’ curricula and the actions developed on this topic in the Greek geoscientific community. In the Greek educational reality, geoeducation is not widespread, and geoethics is used in limited school curricula. The research highlighted a significant lack of initiatives to promote geoethical thinking and the values related to geological heritage and the need to protect it.
Hydrogeochemical Characteristics and Isotopic Tools Used to Identify the Mineralization Processes of Bottled Mineral Water in Morocco
Authors: Ghalit Mohammad , Bouaissa Mohamed , Gharibi Elkhadir , Taupin Jean-Denis , Patris Nicolas
Published: January 2023

Bottled waters are among the most important products in the human diet. In Morocco, eleven bottled mineral waters were analyzed (physicochemical composition and water stable isotopes) to identify their geologic origins and hydrochemical characteristics. The study of the ionic ratios shows that the mechanisms of water–rock interaction, such as the dissolution of carbonate and silicate minerals, influence the chemistry of these mineral waters, which are controlled by lithology and tectonics, sometimes with the influence of deep flux through fractures. The water from the Oulmes plateau shows a Na-HCO3 chemical facies in relationship with alkaline granite. Moreover, the carbonated waters (EMG and EMGL) are characterized by high concentrations of Si, Li, Rb, Sr, and Cs due to deep CO2 flux origin. The waters of Saiss, Middle Atlas, and Rif are of the Ca-HCO3 type linked mainly to carbonate formation. The stable isotope contents of water range from −7.74 to −5.35‰ for δ18O and −45.4 to −30.4‰ for δD. The recharge altitude of the aquifer was estimated to be between 250 and 1200 m, following a δ18O altitudinal gradient between 0.25 and 0.27‰ per 100 m. The industrial production process and the natural presence or artificial addition of CO2 can also influence the isotopic composition of water. These results can be a relevant guide to decision makers for better exploitation and assessment of the water quality.
Worldwide Trends in Methods and Resources Promoting Geoconservation, Geotourism, and Geoheritage
Authors: Quesada-Valverde Michael E. , Quesada-Román Adolfo
Published: January 2023

This study aims to provide a systematic analysis of the literature of methods and resources supporting geoconservation and geotourism worldwide, while identifying current and future trends in the field. This paper offers a comprehensive bibliometric analysis which comprises the period of 2011–2021 after an in-depth systematic literature review of 169 papers, using Web of Science. The volume of research on these topics is growing rapidly, especially in Italy, Poland, Brazil, Russia, and China; these constitute the most productive countries. The main identified geomorphological environments are sedimentary, volcanic, aeolian, coastal, fluvial, and karstic. We discovered that the main methods for evaluating geoconservation and geotourism are geomorphological mapping, the study of economic values for geotourism, field work as a research tool, geoheritage management, documentation, exploration, and inventories of geoheritage at a regional level. The main determined resources are UNESCO Geoparks, educational activities, digital tools, geomanagement, economic values, geoitineraries, and geoeducation programs. To our knowledge, this is the first study dealing with methods and resources publicizing geoconservation and geotourism, worldwide. Knowing about the most successful methods and resources for promoting geoconservation and geotourism can definitely be useful for future endeavors in countries where geoheritage studies are starting to be developed.
The Infierno Glacier (Pyrenees, Aragon, Spain): Evolution 2016–2022
Authors: Cancer-Pomar Luis , Fernández-Jarne Gonzalo , Cuchí José Antonio , del Valle-Melendo Javier
Published: January 2023

The Infierno Glacier is located in Aragon (Spain), Pyrenees Mountain range, the only one in this country that still preserves white glaciers. These are the southernmost glaciers in Europe and are currently in rapid decline. The work analyzes the evolution of the glacier between 2016 and 2022 and provides data, for this period, which lacked this information, in an area bordering the glacial ice survival. In addition to the observations on the glacier itself, the variables (precipitation, temperature, snow volume and thickness) that allow an understanding of this evolution are studied. The results show a setback of the glacier (thickness losses: 4.6 m; front retreat; 14.9 m). The evolution has frequent trend changes, linked to the interannual climatic irregularity characteristic of the Pyrenees. The main explanatory factor is the thermal increase. The thermal anomalies with respect to the average reference values have increased, in this period, by +0.55 °C. The year 2022 has been particularly warm and has recorded the greatest losses for this glacier. With respect to precipitation, it has an irregular behavior and shows a tendency to decrease (−9% in the same period). This work has the additional interest of analyzing a glacier in the terminal phase, which if current trends continue, evolves into dead ice.