bluuebunny/crossref_metadata_2025 huggingface dataset:
Diet, Gut Microbiota and Non-Alcoholic Fatty Liver Disease: Three Parts of the Same Axis
Authors: Quesada-Vázquez Sergio , Aragonès Gerard , Del Bas Josep M , Escoté Xavier
Published: January 2020

Non-Alcoholic Fatty Liver Disease (NAFLD) is the most common liver disease in the world. NAFLD is principally characterized by an excessive fat accumulation in the hepatocytes. Diet is considered as one of the main drivers to modulate the composition of gut microbiota, which participate in different processes, affecting human metabolism. A disruption in the homeostasis of gut microbiota may lead to dysbiosis, which is commonly reflected by a reduction of the beneficial species and an increment in pathogenic microbiota. Gut and liver are in close relation due to the anatomical and functional interactions led by the portal vein, thus altered intestinal microbiota might affect liver functions, promoting inflammation, insulin resistance and steatosis, which is translated into NAFLD. This review will highlight the association between diet, gut microbiota and liver, and how this axis may promote the development of NAFLD progression, discussing potential mechanisms and alterations due to the dysbiosis of gut microbiota. Finally, it will revise the variations in gut microbiota composition in NAFLD, and it will focus in specific species, which directly affect NAFLD progression.
SSEA3 and Sialyl Lewis a Glycan Expression Is Controlled by B3GALT5 LTR through Lamin A-NFYA and SIRT1-STAT3 Signaling in Human ES Cells
Authors: Cai Bi-He , Lee Hsueh-Yi , Chou Chi-Kan , Wu Po-Han , Huang Hsiang-Chi , Chao Chia-Chun , Chung Hsiao-Yu , Kannagi Reiji
Published: January 2020

B3GALT5 is involved in the synthesis of embryonic stem (ES) cell marker glycan, stage-specific embryonic antigen-3 (SSEA3). This gene has three native promoters and an integrated retroviral long terminal repeat (LTR) promoter. We found that B3GALT5-LTR is expressed at high levels in human ES cells. B3GALT5-LTR is also involved in the synthesis of the cancer-associated glycan, sialyl Lewis a. Sialyl Lewis a is expressed in ES cells and its expression decreases upon differentiation. Retinoic acid induced differentiation of ES cells, decreased the short form of NFYA (NFYAs), increased phosphorylation of STAT3, and decreased B3GALT5-LTR expression. NFYAs activated, and constitutively-active STAT3 (STAT3C) repressed B3GALT5-LTR promoter. The NFYAs and STAT3C effects were eliminated when their binding sites were deleted. Retinoic acid decreased the binding of NFYA to B3GALT5-LTR promoter and increased phospho-STAT3 binding. Lamin A repressed NFYAs and SSEA3 expression. SSEA3 repression mediated by a SIRT1 inhibitor was reversed by a STAT3 inhibitor. Repression of SSEA3 and sialyl Lewis a synthesis mediated by retinoic acid was partially reversed by lamin A short interfering RNA (siRNA) and a STAT3 inhibitor. In conclusion, B3GALT5-LTR is regulated by lamin A-NFYA and SIRT1-STAT3 signaling that regulates SSEA3 and sialyl Lewis a synthesis in ES cells, and sialyl Lewis a is also a ES cell marker.
Corrosion of MgO-C with Magnesium Aluminate Spinel Addition in A Steel Casting Simulator
Authors: Dudczig Steffen , Schmidt Gert , Aneziris Christos G. , Wöhrmeyer Christoph , Parr Christopher , Gehre Patrick
Published: January 2020

For more than 20 years, the sidewalls and bottom of steel ladles have been lined with carbon-bonded magnesia (MgO-C) and magnesia-alumina bricks (MAC). The alumina raw materials react with magnesia forming a spinel, which decreases open porosity and slag infiltration. The amount, grain size, and chemistry of the added spinel impact the properties of spinel-containing MgO-C. Corrosion tests have been performed in a steel casting simulator at 1580 °C using 18CrNiMo7-6 steel and Fe-rich slag as corrosion medium. Digital light microscopy and SEM/ EDS (scanning electron microscope with energy dispersive spectroscopy) were used to evaluate the corrosion mechanisms. The metal casting simulator test showed that the addition of CaO-MgO-Al2O3 aggregates results in the highest corrosion resistance against molten steel and synthetic basic slag compared to alumina-rich spinel aggregates.
Investigation of Photoelastic Property and Stress Analysis for Optical Polyimide Membrane through Stress Birefringence Method
Authors: Gao Guohan , Mao Danbo , Jiang Renkui , Li Zhiwei , Liu Xin , Lei Baiping , Bian Jiang , Wu Shibin , Fan Bin
Published: January 2020

Optical polyimide (PI) membranes have been increasingly attractive in optoelectronic substrate and optical element material applications. Controlled stress distribution is very important to optical PI membrane-based optics. However, nondestructive absolute stress measurement inside optical PI membranes remains challenging. In this letter, we adopted the stress birefringence method to experimentally investigate the correlation between stress and retardation in uniaxially, biaxially, and circularly stretched PI membranes. The calculated value of the photoelastic coefficient was found to be around 400 nm/Mpa·cm. A theoretical model was established where the retardation angle is the negative arctan of the principal stress ratio in the biaxially stretched membrane. We also found that the average retardation angle is an important parameter for evaluating the uniformity of stretching force in the circularly stretched membrane. This work provides a better understanding of the stress birefringence measurement of membrane materials.
Studies of Polylactic Acid and Metal Oxide Nanoparticles-Based Composites for Multifunctional Textile Prints
Authors: Abdelrahman Meram S. , Nassar Sahar H. , Mashaly Hamada , Mahmoud Safia , Maamoun Dalia , El-Sakhawy Mohamed , Khattab Tawfik A. , Kamel Samir
Published: January 2020

A novel approach toward the production of multifunctional printed technical textiles is reported. Three different metal oxides nanoparticles including titanium dioxide, magnesium oxide, and zinc oxide were prepared and characterized. Both natural wool and synthetic acrylic fibers were pretreated with the prepared metal oxide nanoparticles followed by printing using polylactic acid based paste containing acid or basic dyestuffs. Another route was applied via post-treatment of the targeted fabrics with the metal oxide nanoparticles after running the printing process. The color strength (K/S) and colorfastness properties of pretreated and post-treated printed fabrics were evaluated and compared with untreated printed fabrics. The presence of nanoparticles on a fabric surface during the coating process was found to significantly increase the color strength value of the coated textile substrates. The increased K/S value depended mainly on the nature and concentration of the applied metal oxide, as well as the nature of colorant and fabric. In addition, the applied metal oxide nanoparticles imparted the printed fabrics with good antibacterial activity, high ultraviolet protection, photocatalytic self-cleaning, and improved colorfastness properties. Those results suggest that the applied metal oxide-based nanoparticles could introduce ideal multifunctional prints for garments.
Linear and Nonlinear Absorption of Titanium Dioxide Films Produced by Plasma Ion-Assisted Electron Beam Evaporation: Modeling and Experiments
Authors: Stenzel Olaf , Wilbrandt Steffen , Mühlig Christian , Schröder Sven
Published: January 2020

Titanium dioxide films were prepared by plasma ion-assisted electron beam evaporation. Linear optical properties were investigated in terms of spectrophotometry using the beta-distributed oscillator (ß_do) model as a parametrized dispersion law. The nonlinear two-photon absorption coefficient of titanium dioxide was determined by means of the laser-induced deflection technique at a wavelength of 800 nm. The obtained values of (2–5) × 10−11 cm/W were consistent with published experimental values for rutile as well as for simulations performed in the frames of the ß_do and Sheik–Bahae models.
Antireflection Coating on PMMA Substrates by Atomic Layer Deposition
Authors: Paul Pallabi , Pfeiffer Kristin , Szeghalmi Adriana
Published: January 2020

Antireflection coatings (ARC) are essential for various optical components including such made of plastics for high volume applications. However, precision coatings on plastics are rather challenging due to typically low adhesion of the coating to the substrate. In this work, optimization of the atomic layer deposition (ALD) processes towards conformal optical thin films of Al2O3, TiO2 and SiO2 on poly(methyl methacrylate) (PMMA) has been carried out and a five-layer ARC is demonstrated. While the uncoated PMMA substrates have a reflectance of nearly 8% in the visible (VIS) spectral range, this is reduced below 1.2% for the spectral range of 420–670 nm by applying a double-side ARC. The total average reflectance is 0.7%. The optimized ALD coatings show a good adhesion to the PMMA substrates even after the climate test. Microscopic analysis on the cross-hatch areas on PMMA after the climate test indicates very good environmental stability of the ALD coatings. These results enable a possible route by ALD to deposit uniform, crack free, adhesive and environmentally durable thin film layers on sensitive thermoplastics like PMMA.
Influence of Crystallization Behavior of Gas Quenching Blast Furnace Slag on the Preparation of Amorphous Slag Beads
Authors: Kang Yue , Liu Chao , Zhang Yuzhu , Xing Hongwei
Published: January 2020

Slag beads with different crystal content could be obtained through the gas quenching blast furnace slag (BFS) process. In order to increase the additional value of the slag beads as much as possible, it was necessary to restrain the crystallization of the slag beads as much as possible. In this paper, the mineral types and crystallization temperatures of BFS with different basicities and cooling rates were studied by using Factsage thermodynamic software, XRD, and differential scanning calorimeter (DSC) experiments, which obtained the gas quenching temperature and the cooling rate needed to restrain crystallization behavior in the gas quenching process; The crystallization mechanism was studied by calculating crystallization activation energy (Ec) using the DSC experiment, at the same time, the thermodynamic results were verified. The proper basicity and cooling rate of BFS were found to be conducive to the preparation of amorphous slag beads. The results showed that the initial crystallization temperature decreased with decreasing the basicity and increasing the cooling rate, which could increase the amorphous content of slag beads in the gas quenching process. The crystallization activation energy (Ec) increased with decreasing basicity, which increased the crystallization barrier.
Detection of Hypoglycemia Using Measures of EEG Complexity in Type 1 Diabetes Patients
Authors: Rubega Maria , Scarpa Fabio , Teodori Debora , Sejling Anne-Sophie , Frandsen Christian S. , Sparacino Giovanni
Published: January 2020

Previous literature has demonstrated that hypoglycemic events in patients with type 1 diabetes (T1D) are associated with measurable scalp electroencephalography (EEG) changes in power spectral density. In the present study, we used a dataset of 19-channel scalp EEG recordings in 34 patients with T1D who underwent a hyperinsulinemic–hypoglycemic clamp study. We found that hypoglycemic events are also characterized by EEG complexity changes that are quantifiable at the single-channel level through empirical conditional and permutation entropy and fractal dimension indices, i.e., the Higuchi index, residuals, and tortuosity. Moreover, we demonstrated that the EEG complexity indices computed in parallel in more than one channel can be used as the input for a neural network aimed at identifying hypoglycemia and euglycemia. The accuracy was about 90%, suggesting that nonlinear indices applied to EEG signals might be useful in revealing hypoglycemic events from EEG recordings in patients with T1D.
Smart Public Lighting Control and Measurement System Using LoRa Network
Authors: Sánchez Sutil F. , Cano-Ortega Antonio
Published: January 2020

The installation of smart meters in smart cities to monitor streetlights (SLs) provides easy access to measurements of electrical variables and lighting levels, which improves the operation of installation. The use of smart meters in cities requires temporary high-resolution data to improve the energy efficiency (EE) of SLs. Long range (LoRa) is an ideal wireless protocol for use in smart cities due to its low energy consumption, secure communications, and long range indoors and outdoors. For this purpose, we developed a low-cost new system and successfully evaluated it by developing three devices, namely the measure and control device for street lights (MCDSL), lighting level measurement device (LLMD) and gateway LoRa network (GWLN), based on the Arduino open-source electronic platform. This paper describes the hardware and software design and its implementation. Further, an algorithm has been developed to enhance the energy efficiency of public lights using MCDSL, the energy efficiency for street lights (EESL) algorithm, that use the illumination level measured on the same set of SLs with a dynamic control, which assumed different lighting levels throughout the night, and adjusted luminous flux based on the traffic intensity of pedestrians. It sends the acquired data through the LoRa low-power wide-area-network (LPWAN) to the cloud.
A Hybrid PV-Battery/Supercapacitor System and a Basic Active Power Control Proposal in MATLAB/Simulink
Authors: Şahin Mustafa Ergin , Blaabjerg Frede
Published: January 2020

An increase in the integration of renewable energy generation worldwide brings along some challenges to energy systems. Energy systems need to be regulated following grid codes for the grid stability and efficiency of renewable energy utilization. The main problems that are on the active side can be caused by excessive power generation or unregulated energy generation, such as a partially cloudy day. The main problems on the load side can be caused by excessive or unregulated energy demand or nonlinear loads which deteriorate the power quality of the energy networks. This study focuses on the energy generation side as active power control. In this study, the benefits of supercapacitor use in a hybrid storage system are investigated and analyzed. A hybrid system in which photovoltaic powered and stored the energy in battery and supercapacitor are proposed in this study to solving the main problems in two sides. The supercapacitor model, photovoltaic model, and the proposed hybrid system are designed in MATLAB/Simulink for 6 kW rated power. Also, a new topology is proposed to increase the energy storage with supercapacitors for a passive storage system. The instantaneous peak currents energy is aimed to store in supercapacitors temporarily with this topology. The main advantages of this topology are voltage stabilization in two sides by the supercapacitors and a limitation of the battery load, which directly results in longer battery life and decreases the system cost. The simulation results are investigated for this topology.
Wake Structures and Surface Patterns of the DrivAer Notchback Car Model under Side Wind Conditions
Authors: Wieser Dirk , Nayeri Christian Navid , Paschereit Christian Oliver
Published: January 2020

The flow field topology of passenger cars considerably changes under side wind conditions. This changes the surface pressure, aerodynamic force, and drag and performance of a vehicle. In this study, the flow field of a generic passenger vehicle is investigated based on three different side wind angles. The study aimed to identify vortical structures causing changes in the rear pressure distribution. The notchback section of the DrivAer model is evaluated on a scale of 1:4. The wind tunnel tests are conducted in a closed section with a splitter plate at a Reynolds number of 3 million. The side wind angles are 0 ∘ , 5 ∘ , and 10 ∘ . The three-dimensional and time-averaged flow field downstream direction of the model is captured by a stereoscopic particle image velocimetry system performed at several measurement planes. These flow field data are complemented by surface flow visualizations performed on the entire model. The combined approaches provide a comprehensive insight into the flow field at the frontal and side wind inflows. The flow without side wind is almost symmetrical. Longitudinal vortices are evident along the downstream direction of the A-pillar, the C-pillars, the middle part of the rear window, and the base surface. In addition, there is a ring vortex downstream of the vehicle base. The side wind completely changes the flow field. The asymmetric topology is dominated by the windward C-pillar vortex, the leeward A-pillar vortex, and other base vortices. Based on the location of the vortices and the pressure distributions measured in earlier studies, it can be concluded that the vortices identified in the wake are responsible for the local minima of pressure, increasing the vehicle drag.
Effects of the Bore to Stroke Ratio on Combustion, Gaseous and Particulate Emissions in a Small Port Fuel Injection Engine Fueled with Ethanol Blended Gasoline
Authors: Jang Jihwan , Choi Jonghui , Yi Hoseung , Park Sungwook
Published: January 2020

The purpose of this study is to analyze the combustion characteristics of the port fuel injection (PFI) engine considering the fuel mixing ratio, bore to stroke (B/S) ratio and gaseous and particle emissions. Experiments were conducted in a small single-cylinder PFI engine with a displacement of 125 cc. The fuel used in the experiment was a mixture of pure gasoline and ethanol. The engine was operated at 5000 rpm at full load and wide-open throttle. In addition, combustion and exhaust characteristics of the engines with a B/S ratio of 0.88 and 1.15 were analyzed. The combustion pressure inside the combustion chamber was measured to analyze the indicated mean effective pressure (IMEP) and the heat release rate, and the combustion rate was calculated. In the results of combustion characteristics by the difference of B/S ratio, the influence of flame propagation velocity and turbulence intensity is the largest. The 0.88 B/S ratio engine, which has a small bore, has a faster combustion rate than the 1.15 B/S ratio engine due to its larger flame surface area and larger turbulence intensity. This represents a higher efficiency combustion result. Finally, the high oxygen content of ethanol has the characteristic of decreasing soot formation and increasing particle oxidation.
Architectures of Planetary Hybrid Powertrain System: Review, Classification and Comparison
Authors: Wang Lihua , Cui Yahui , Zhang Fengqi , Li Guanglei
Published: January 2020

Increasing environmental issues and energy crises led to rapid developments of hybrid electric vehicles, especially the planetary hybrid powertrain system (PHPS). This paper presents a comprehensive review of the PHPS, focusing primarily on contributions in the aspect of configuration, classification and comparison. In this work, a new classification method for PHPS architectures is proposed according to the number of electric motors (EMs). In addition, two kinds of PHPS, in the new classification framework, are extensively emphasized in terms of its architectures, advantages and disadvantages. Furthermore, the port diagrams of representative architectures are presented to provide an intuitive method for power flow representation. Finally, a conclusion is made to provide an insight for developing PHPS as well.
Active Reduction of Short-Circuit Current in Power Distribution Systems
Authors: Pulido Esteban , Morán Luis , Villarroel Felipe , Silva José
Published: January 2020

In this paper, a new concept of short-circuit current (SCC) reduction for power distribution systems is presented and analyzed. Conventional fault current limiters (FCLs) are connected in series with a circuit breaker (CB) that is required to limit the short-circuit current. Instead, the proposed scheme consisted of the parallel connection of a current-controlled power converter to the same bus intended to reduce the amplitude of the short-circuit current. This power converter was controlled to absorb a percentage of the short-circuit current from the bus to reduce the amplitude of the short-circuit current. The proposed active short-circuit current reduction scheme was implemented with a cascaded H-bridge power converter and tested by simulation in a 13.2 kV industrial power distribution system for three-phase faults, showing the effectiveness of the short-circuit current attenuation in reducing the maximum current requirement in all circuit breakers connected to the same bus. The paper also presents the design characteristics of the power converter and its associated control scheme.
Biomass and Lipid Production Potential of an Indian Marine Algal Isolate Tetraselmis striata BBRR1
Authors: Boopathy Annakkili Baskara , Jayakumar Thanasekaran , Chinnasamy Senthil , Rajaram Muthu Ganesan , Mohan Natarajan , Nagaraj Subramani , Rengasamy Ramasamy , Manubolu Manjunath , Sheu Joen-Rong , Chang Chao-Chien
Published: January 2020

Four different strains of marine algae viz. Tetraselmis tetrathele, Tetraselmis striata, Tetraselmis chuii, and Tetraselmis gracilis were isolated from the saltpans in Kovelong, Chennai, Tamil Nadu, India. The systematic position of Tetraselmis striata BBRR1 was confirmed through molecular identification. Under laboratory conditions, T. striata Butcher BBRR1 grown in f/2-medium recorded highest biomass concentration of 0.58 ± 0.021 g L−1, volumetric productivity of 0.025 ± 0.004 g L−1 d−1, 19 ± 2.3% proteins, 17 ± 1.5% carbohydrates, and 15 ± 2.4% lipids. Volumetric biomass productivity of 0.063 ± 0.08 g L−1 d−1, specific growth rate of 0.45 day−1 and lipid content of 19.42 ± 0.98% were recorded for the alga T. striata Butcher BBRR1 cultivated in 10-m2 open raceway ponds using Modified CFTRI ABRR1 medium. The fatty acid profile of T. striata Butcher BBRR1 showed the presence of 33.14% palmitic acid, 22.64% 11-octadecenoic acid, and 21.94% heptadecanoic acid. This study confirms the feasibility of cultivating the marine alga T. striata in open raceway ponds to produce biomass, which can be used for the production of biofuels.
Analysis of Model Predictive Current-Controlled Permanent Magnet Synchronous Motor Drives with Inaccurate DC Bus Voltage Measurement
Authors: Wang Wei , Lu Zhixiang
Published: January 2020

In this paper, the effects of inaccurate DC bus voltage measurement are analyzed to model predictive current-controlled permanent magnet synchronous motor (PMSM) drives. It is found that the selection of the optimal space vector is affected by inaccurate DC bus voltage measurements, and the shortest distance principle is proposed to evaluate the effects. With the under-voltage measurement, the actual q axis current is always larger than the reference value, and PMSM may be damaged by the over-current phenomenon. However, the actual q axis current is always smaller than the reference value with the over-voltage measurement, and the rated torque capacity cannot be properly used. The effects of the over-voltage measurement are more serious than those of the under-voltage measurement. Additionally, the larger DC bus voltage measurement error can result in more serious effects than with the over-voltage measurement. Considering the limited variation range of the actual DC bus voltage, the rated value can be set as the measured value and the effects can be neglected. However, the effects should be taken into account if the variation range of the actual DC bus voltage is large. All the theoretical analyses are verified by experimental results.
Selective Electromagnetic Measurements of 4G Signals: Results of an Italian National Intercomparison
Authors: Ardoino Lucia , Adda Sara , Anglesio Laura , Barbieri Enrichetta
Published: January 2020

In June 2016, with the aim of ensuring a global improvement in the performance of the Italian System of the Environmental Agencies (SNPA) and its homogeneity on the national territory, an intercomparison circuit (IC) was planned and conducted concerning the measurements of electromagnetic fields associated with Long Term Evolution (LTE) mobile communications, which were very recently introduced at that time. The intercomparison circuit, designed and built according to the criteria of ISO 17043, was organized as part of a consolidated collaboration between the Institute for Environmental Protection and Research (ISPRA) and the Piedmont Regional Agency for Environmental Protection (Arpa Piemonte). The results obtained, preceded by a brief description of the entire process of organization and analysis, are the subject of this work. The IC covered in particular: the narrow band measurement procedures used in the field; the choice of decoding, measurement and extrapolation of the synthesis result; the response of the instrumentation, limited to the models in the field. The site chosen by the organizers, primarily characterized through measurements and theoretical evaluation of the field, is the roof of the Lingotto Building in Turin. A total of 27 groups participated in the circuit: 25 SNPA departments (including the organizers Arpa Piemonte and ISPRA) and 2 private labs. All participants provided the results. The outcome of the comparison was decidedly positive: only 2 participants, for whom a joint assessment of possible causes will also be illustrated (according to ISO 5725:2), achieved significantly different results.
The Importance of Multi-Scale Temporal and Spatial Management for Cemetery Trees in Malmö, Sweden
Authors: Quinton Jessica M. , Östberg Johan , Duinker Peter N.
Published: January 2020

Research Highlights: A large body of research highlighted the important contributions that urban forests make to cities and their inhabitants. However, our urban forests face threats from issues such as rapid urbanization, climate change, and the spread of pests and diseases. As such, proactive and effective management is necessary to ensure their long-term sustainability. Given the multiple spatial and temporal scales on which threats can arise, effective management needs to account for these scales and adjust accordingly. The degree to which this currently happens is unclear. Background and Objectives: The aim of this study was to determine the role of multi-scale management in urban forestry, using cemeteries in Malmö, Sweden as a case study. Cemeteries can provide extensive tree canopy but are not readily considered in urban forest management. We sought to determine (1) the threats to the current cemetery tree populations, (2) the extent of multi-scale cemetery tree management, (3) whether tree management plans promote multi-scale management, and (4) how cemetery tree management can be improved. Materials and Methods: Malmö cemetery tree inventories were analyzed with respect to size class and species diversity. Existing cemetery tree management plans were examined to determine the spatial and temporal scales of their recommendations. Interviews were conducted with cemetery managers to determine management priorities and actions. Results: We found that cemetery tree populations in Malmö suffer from a lack of age class and species diversity. Management tends to occur on short time scales and efforts focus mainly on addressing individual trees, although some consideration is given to large-scale species diversification. The management plans previously created for these cemetery trees make recommendations for age class and species diversification but are yet to be used extensively by cemetery managers. Conclusions: The long-term stability of Malmö’s cemetery tree populations is threatened by a lack of species and age diversity. Current management efforts emphasize addressing small-scale issues. Although there is a desire to improve species diversity, this can cause conflict with existing cultural values.
SLAF-seq Uncovers the Genetic Diversity and Adaptation of Chinese Elm (Ulmus parvifolia) in Eastern China
Authors: Lyu Yun-zhou , Dong Xiao-yun , Huang Li-bin , Zheng Ji-wei , He Xu-dong , Sun Hai-nan , Jiang Ze-ping
Published: January 2020

The Chinese elm is an important tree ecologically; however, little is known about its genetic diversity and adaptation mechanisms. In this study, a total of 107 individuals collected from seven natural populations in eastern China were investigated by specific locus amplified fragment sequencing (SLAF-seq). Based on the single nucleotide polymorphisms (SNPs) detected by SLAF-seq, genetic diversity and markers associated with climate variables were identified. All seven populations showed medium genetic diversity, with PIC values ranging from 0.2632 to 0.2761. AMOVA and Fst indicated that a low genetic differentiation existed among populations. Environmental association analyses with three climate variables (annual rainfall, annual average temperature, and altitude) resulted in, altogether, 43 and 30 putative adaptive loci by Bayenv2 and LFMM, respectively. Five adaptive genes were annotated, which were related to the functions of glycosylation, peroxisome synthesis, nucleic acid metabolism, energy metabolism, and signaling. This study was the first on the genetic diversity and local adaptation in Chinese elms, and the results will be helpful in future work on molecular breeding.
Chemical Structure and Mechanical Properties of Wood Cell Walls Treated with Acid and Alkali Solution
Authors: Xu Enguang , Wang Dong , Lin Lanying
Published: January 2020

The mechanical properties of individual fibers are related to the production and performance of papers and fiberboards. This paper examines the behavior of the microstructure constituents of wood subjected to acid and alkali treatments. The chemical structure and mechanical properties of wood cell walls with different acid or alkali treatments was analyzed. The results show that, compared with acid treatment, the crystal size and crystallinity index of cellulose increased after alkali treatment, resulting in an increase in the cell wall elastic modulus. The mechanical properties of the wood cell wall S2 region were higher than those of the compound middle lamella (CML) region. There was a topochemical effect between the CML and the S2 region in acid and alkali-treated samples, which provided a major threshold that facilitates the production/separation of wood fibers for better strength fiber properties.
Irrigation of Young Cork Oaks under Field Conditions—Testing the Best Water Volume
Authors: Camilo-Alves Constança , Dinis Cati , Vaz Margarida , Barroso João M. , Ribeiro Nuno Almeida
Published: January 2020

This study is the beginning of the first long-term study on cork oak irrigation under field conditions, with a structural-functional approach. Cork oaks are currently facing disturbances affecting cork quality and quantity, jeopardizing the future of the economic sector. There is a need for new production techniques that maximize cork oak growth and vitality. In this study, irrigation was implemented in a new intensive cork oak plantations to test the best irrigation volume. The long-term goal is to improve tree growth with minimum water requirements. A 6 ha intensive plantation was installed in Coruche, Portugal. The experimental plot consisted of a subsurface drip fertigation system, buried 40 cm deep; with five independent irrigation treatments. It was tested four irrigation volumes during the dry period—21 weeks in the summer of 2016—ranging from 1.88 mm to 5.62 mm a week. Information on meteorological conditions, soil moisture profile and leaf stomatal conductance were gathered periodically and dendrometric measurements were performed before and after the treatments. Cork oaks’ structural and functional parameters were associated with irrigation volume Response to irrigation showed an inflection point in treatment 2, corresponding to a water supply of 3.12 mm per week: below the inflection point, stomatal conductance was reduced by 15% and relative diameter growth at the base was reduced by 10%. Stomatal conductance also showed a positive relationship with soil moisture below the irrigation tubes and with plants’ stem diameter. In conclusion, irrigation supply during the period of water stress improved function and structure of cork oaks seedlings under field conditions. These results suggest that irrigation can be a viable alternative to improve cork oak growth in afforestation and reforestation.
CFRP Laminates Reinforcing Performance of Short-Span Wedge-Blocks Segmental Beams
Authors: Abdulhameed Ali A. , Said AbdulMuttalib I.
Published: January 2020

Two of the main advantages of segmental construction are economics, as well as the rapid construction technique. One of the forms of segmental construction, for structural elements, is the segmental beams that built-in short sections, which referred to segments. This research aims to exhibit a new technique for the fabrication of short-span segmental beams from wedge-shaped concrete segments and carbon fiber reinforced polymers (CFRP) in laminate form. The experimental campaign included eight short-span segmental beams. In this study, two selected parameters were considered. These parameters are; the number of layers of CFRP laminates and the adhesive material that used to bond segments to each other, forming short-span segmental beams. The test results showed that for segmental beams reinforced by 2-layer of CFRP laminates, undergoes less deflection and sustained considerable ultimate loading value of 38.4%–104% than beams reinforced by 1-layer. Moreover, the test of segmental beams fabricated by adhering to the concrete segments with epoxy resin exhibited an increase in ultimate loading by 16%–65% than beams constructed using cementitious adhesive for bonding the wedge-shaped segments. Theoretically, segmental beams were analyzed by the American Concrete Institute (ACI) 440.2R-17 procedure with slight modifications. The analysis gave an overestimation of flexural strength for segmental beams when compared with experimental outcomes.
Decoding the Role of Satellite DNA in Genome Architecture and Plasticity—An Evolutionary and Clinical Affair
Authors: Louzada Sandra , Lopes Mariana , Ferreira Daniela , Adega Filomena , Escudeiro Ana , Gama-Carvalho Margarida , Chaves Raquel
Published: January 2020

Repetitive DNA is a major organizational component of eukaryotic genomes, being intrinsically related with their architecture and evolution. Tandemly repeated satellite DNAs (satDNAs) can be found clustered in specific heterochromatin-rich chromosomal regions, building vital structures like functional centromeres and also dispersed within euchromatin. Interestingly, despite their association to critical chromosomal structures, satDNAs are widely variable among species due to their high turnover rates. This dynamic behavior has been associated with genome plasticity and chromosome rearrangements, leading to the reshaping of genomes. Here we present the current knowledge regarding satDNAs in the light of new genomic technologies, and the challenges in the study of these sequences. Furthermore, we discuss how these sequences, together with other repeats, influence genome architecture, impacting its evolution and association with disease.
Bone Marrow Clonogenic Myeloid Progenitors from NPM1-Mutated AML Patients Do Not Harbor the NPM1 Mutation: Implication for the Cell-Of-Origin of NPM1+ AML
Authors: Diaz de la Guardia Rafael , González-Silva Laura , López-Millán Belén , Rodríguez-Sevilla Juan José , Baroni Matteo L. , Bueno Clara , Anguita Eduardo , Vives Susana , Palomo Laura , Lapillonne Helene , Varela Ignacio , Menendez Pablo
Published: January 2020

The cell-of-origin of NPM1- and FLT3-mutated acute myeloid leukemia (AML) is still a matter of debate. Here, we combined in vitro clonogenic assays with targeted sequencing to gain further insights into the cell-of-origin of NPM1 and FLT3-ITD-mutated AML in diagnostic bone marrow (BM) from nine NPM1+/FLT3-ITD (+/−) AMLs. We reasoned that individually plucked colony forming units (CFUs) are clonal and reflect the progeny of a single stem/progenitor cell. NPM1 and FLT3-ITD mutations seen in the diagnostic blasts were found in only 2/95 and 1/57 individually plucked CFUs, suggesting that BM clonogenic myeloid progenitors in NPM1-mutated and NPM1/FLT3-ITD-mutated AML patients do not harbor such molecular lesions. This supports previous studies on NPM1 mutations as secondary mutations in AML, likely acquired in an expanded pool of committed myeloid progenitors, perhaps CD34−, in line with the CD34−/low phenotype of NPM1-mutated AMLs. This study has important implications on the cell-of-origin of NPM1+ AML, and reinforces that therapeutic targeting of either NPM1 or FLT3-ITD mutations might only have a transient clinical benefit in debulking the leukemia, but is unlikely to be curative since will not target the AML-initiating/preleukemic cells. The absence of NPM1 and FLT3-ITD mutations in normal clonogenic myeloid progenitors is in line with their absence in clonal hematopoiesis of indeterminate potential.
Mitochondrial Genome Sequencing Reveals orf463a May Induce Male Sterility in NWB Cytoplasm of Radish
Authors: Wang Yanping , Wang Qingbiao , Hao Wei , Li Jianxia , Qi Meixia , Zhang Li
Published: January 2020

Radish (Raphanus sativus L.) is an important root vegetable worldwide. The development of F1 hybrids, which are extensively used for commercial radish production, relies on cytoplasmic male sterility (CMS). To identify candidate genes responsible for CMS in NWB cytoplasm, we sequenced the normal and NWB CMS radish mitochondrial genomes via next-generation sequencing. A comparative analysis revealed 18 syntenic blocks and 11 unique regions in the NWB CMS mitogenome. A detailed examination indicated that orf463a was the most likely causal factor for male sterility in NWB cytoplasm. Interestingly, orf463a was identical to orf463, which is responsible for CMS in Dongbu cytoplasmic and genic male sterility (DCGMS) radish. Moreover, only structural variations were detected between the NWB CMS and DCGMS mitochondrial genomes, with no nucleotide polymorphisms (SNPs) or meaningful indels. Further analyses revealed these two mitochondrial genomes are coexisting isomeric forms belonging to the same mitotype. orf463a was more highly expressed in flower buds than in vegetative organs and its expression was differentially regulated in the presence of restorer of fertility (Rf) genes. orf463a was confirmed to originate from Raphanus raphanistrum. In this study, we identified a candidate gene responsible for the CMS in NWB cytoplasm and clarified the relationship between NWB CMS and DCGMS.
Mitochondrial DNA Variation and Selfish Propagation Following Experimental Bottlenecking in Two Distantly Related Caenorhabditis briggsae Isolates
Authors: Wagner Josiah T. , Howe Dana K. , Estes Suzanne , Denver Dee R.
Published: January 2020

Understanding mitochondrial DNA (mtDNA) evolution and inheritance has broad implications for animal speciation and human disease models. However, few natural models exist that can simultaneously represent mtDNA transmission bias, mutation, and copy number variation. Certain isolates of the nematode Caenorhabditis briggsae harbor large, naturally-occurring mtDNA deletions of several hundred basepairs affecting the NADH dehydrogenase subunit 5 (nduo-5) gene that can be functionally detrimental. These deletion variants can behave as selfish DNA elements under genetic drift conditions, but whether all of these large deletion variants are transmitted in the same preferential manner remains unclear. In addition, the degree to which transgenerational mtDNA evolution profiles are shared between isolates that differ in their propensity to accumulate the nduo-5 deletion is also unclear. We address these knowledge gaps by experimentally bottlenecking two isolates of C. briggsae with different nduo-5 deletion frequencies for up to 50 generations and performing total DNA sequencing to identify mtDNA variation. We observed multiple mutation profile differences and similarities between C. briggsae isolates, a potentially species-specific pattern of copy number dysregulation, and some evidence for genetic hitchhiking in the deletion-bearing isolate. Our results further support C. briggsae as a practical model for characterizing naturally-occurring mtgenome variation and contribute to the understanding of how mtgenome variation persists in animal populations and how it presents in mitochondrial disease states.
‘There’s No Return Route, Is There?’: Conor O’Callaghan’s After-Irish Diasporic Aesthetic
Authors: Wortley Alexander
Published: January 2020

In this article, I examine Conor O’Callaghan’s poetry in the context of post- or after-Irishness and migration. The idea of a traditional Irish national literature has diminished in importance and relevance in recent years. Irish writers are now more sensitized to alternative modes of identification, unbound by the constraints of a singular concept of ‘Irishness’. This is especially significant for migrant writers, who are geographically removed from Ireland. O’Callaghan (born 1968) is himself a migrant: having lived in America, he now lives in England. Drawn from his experiences of transnational migration, O’Callaghan explores the different locales that he has known. He also feels free to write about suburban life, love, and the internet in an often quick-witted vernacular. What then is O’Callaghan’s aesthetic response to the experience of migrancy? Does O’Callaghan’s poetry exhibit an after-Irish diasporic aesthetic? Although O’Callaghan’s poetry is imbued with a diasporic multi-locatedness, both intellectual and geographical, his sense of Irish identity remains strong, and his poetry also often expresses a desire for rootedness.
Why the West Could Not Hear Beale Street: Baldwin’s World-Sense of Female Sexuality
Authors: Yeboah Amy
Published: January 2020

While scholars have noted James Baldwin’s revisionary and transformative literary approach to social constructions of race, class, gender, and crime, there has been very little conversation in that vein regarding If Beale Street Could Talk (1974). Upon its publication, many critics issued negative reviews of the novel, failing to recognize how Baldwin’s view of female sexuality both embraced notions of the body and constructs from an African-centered world-sense. Using a range of theoretical resources from Africana Studies, this paper analyzes how moving beyond Western frameworks regarding knowledge, sexual discourse, and behavior offers a new interpretation of Baldwin’s aims that reclaims and re-imagines Black sexual politics.
Diversity and Antimicrobial Activity of Culturable Fungal Endophytes in Solanum mauritianum
Authors: Pelo Sharon , Mavumengwana Vuyo , Green Ezekiel
Published: January 2020

Plant endophytes are microbial sources of bioactive secondary metabolites, which mimic the natural compounds chemistry of their respective host plants in a similar manner. This study explored the isolation and identification of fungal endophytes, and investigated the antibacterial and antimycobacterial activity of their crude extracts. Fungal endophytes were isolated from Solanum mauritianum, identified using morphological traits and internal transcribed spacer ribosomal-deoxyribonucleic acid (ITS-rDNA) sequence analysis. Eight fungal endophytes were identified as Aureobasidium pullulans, Paracamarosporium leucadendri, Cladosporium sp., Collectotrichum boninense, Fusarium sp., Hyalodendriella sp., and Talaromyces sp., while Penicillium chrysogenum was isolated from the leaves and unripe fruits. Good activity was observed for the crude extracts of Paracamarosporium leucadendri inhibiting Mycobacterium bovis, Klebsiella pneumoniae, and Pseudomonas aeruginosa at 6 µg/mL. Crude extracts of Fusarium sp., showed activity at 9 μg/mL against M. bovis, M. smegmatis and K. pneumonia. In general, the crude extracts showed great activity against Gram-negative and Gram-positive bacteria and novel results for two mycobacteria species M. bovis and M. smegmatis. The results provide evidence of diverse fungal endophytes isolated from Solanum mauritianum, and evidence that fungal endophytes are a good source of bioactive compounds with pharmaceutical potential, particularly against Mycobacterium tuberculosis.
Comparison of Behavioral Changes and Brain Activity between Adolescents with Internet Gaming Disorder and Student Pro-Gamers
Authors: Kwak Ki Hyeon , Hwang Hyun Chan , Kim Sun Mi , Han Doug Hyun
Published: January 2020

While pro-gamers play according to defined living habits and planned schedules, adolescents with internet gaming disorder (IGD) exhibit irregular lifestyles and unregulated impulsive gaming behavior. Fourteen IGD adolescents and 12 pro-gaming students participated in this study. At baseline and after one year, demographic data, the Child Behavior Check List (CBCL), depressed mood, anxiety, and resting-state functional magnetic resonance imaging were assessed. Over the year, IGD adolescents played games as per their usual schedule, while pro-gamer students played according to their school’s team schedule. After one year, the pro-gamers’ scores had decreased in the CBCL-total (total problematic behaviors), CBCL-externalizing (under-controlled behavior, like impulsivity and aggression), and CBCL-internalizing (over-controlled behavior like depression and anxiety) compared to those of the IGD adolescents. Both groups displayed increased brain activity in the parietal lobe (a component of the attention network) over the years. Compared to pro-gamers, IGD adolescents showed higher brain activity within the left orbitofrontal cortex. Brain activity within the orbitofrontal cortex was associated with CBCL-externalizing scores. These results suggest that gaming had increased the attention network’s brain activity, but a well-organized support system could lead to different results, in terms of improved behaviors and suppressing brain activity within the orbitofrontal cortex.
Effect of Chinese Propolis as an Intracanal Medicament on Post-Operative Endodontic Pain: A Double-Blind Randomized Controlled Trial
Authors: Shabbir Juzer , Qazi Fazal , Farooqui Waqas , Ahmed Shahbaz , Zehra Tazeen , Khurshid Zohaib
Published: January 2020

Propolis is a potent anti-microbial and natural anti-inflammatory by-product obtained from the beehive. Studies have demonstrated the superior biocompatibility and anti-microbial properties of propolis as compared to calcium hydroxide. However, its effect on postoperative endodontic pain is unknown. Therefore, this study aimed to investigate the impact of Chinese propolis paste as an intracanal medicament on postoperative endodontic pain intensities compared with calcium hydroxide (control) at different time intervals in necrotic teeth with periapical radiolucency. Eighty patients with single-rooted necrotic teeth with visible periapical radiolucency were recruited and randomly allocated to either the calcium hydroxide or propolis groups. After chemo-mechanical preparation and intracanal medicament insertion, patients were given the VAS (visual analogue scale) to record pain scores. Inter-group data were compared and analyzed using two-way repeated measure ANOVA (Bonferroni test). A p-value of < 0.025 was considered significant. In total, >78% of the patients experienced no or only mild post-operative pain in both the groups at all time intervals, without any significant difference in pain scores between the two groups (p > 0.025). An overall flare-up rate of 14.8% was found. The results suggest that either of these medicaments can be used as an inter-appointment medication for the prevention of postoperative pain in necrotic cases.