bluuebunny/crossref_metadata_2025 huggingface dataset:
Spectrophotometric Study of Bridging N-Donor Ligand-Induced Supramolecular Assembly of Conjugated Zn-Trisporphyrin with a Triphenylamine Core
Authors: Shee Nirmal K. , Seo Ju-Won , Kim Hee-Joon
Published: August 2021

This article studies the supramolecular assembly behavior of a Zn-trisporphyrin conjugate containing a triphenylamine core (1) with bridging N-donor ligands using the UV-vis spectrophotometric titration method at micromolar concentrations. Our results show that pyridine, a non-bridging ligand, formed a 3:1 open complex with 1. The corresponding binding constant was estimated to be (2.7 ± 0.15) × 1014 M−3. In contrast, bridging ligands, 4,4-bipyridine (BIPY) and 1,3-di(4-pyridyl)propane (DPYP), formed stable 3:2 double-decker complexes with 1 in solution, which collapsed to yield a 3:1 open complex when excess BIPY or DPYP was added. The binding constants for forming BIPY and DPYP double-decker complexes were estimated to be (9.26 ± 0.07) × 1027 M−4 and (3.62 ± 0.16) × 1027 M−4, respectively. The UV-vis titration profiles supported the conclusion that the degradation of the 3:2 double-decker 1∙BIPY complex is less favorable compared to that of 1∙DPYP. Consequently, the formation of the 3:1 1∙DPYP open complex proceeded more readily than that of 1∙BIPY.
The Molecular Mechanism of the Formation of Four-Membered Cyclic Nitronates and Their Retro (3 + 2) Cycloaddition: A DFT Mechanistic Study
Authors: Kącka-Zych Agnieszka
Published: August 2021

In the present work, the formation of the four-membered cyclic nitronates and the retro (3 + 2) cycloaddition (retro-32CA) reaction of the 4H-[1,2]oxazete 2-oxide were studied using the density functional theory method at the MPWB1K/6-311G(d,p) theoretical level. The electronic structure of 3-tert-butyl-4,4-dimethyl-1,2-dinitro-pent-2-ene was known through electron localization function analysis, natural population analysis, and molecular electrostatic potential analysis. The formation of 4,4-di-tert-butyl-3-nitromethyl-4H-[1,2]oxazete 2-oxide proceeds through a one-step mechanism. The mechanism of the retro-32CA leading to di-tert-butyl ketone and nitrile oxide derivative should be described as an asynchronous two-stage one-step process. The bonding evolution theory study was carried out to clarify the mechanisms of the formation of 4H-[1,2]oxazete 2-oxide and their retro-32CA.
Magnesium-Free Immobilization of DNA Origami Nanostructures at Mica Surfaces for Atomic Force Microscopy
Authors: Xin Yang , Zargariantabrizi Amir Ardalan , Grundmeier Guido , Keller Adrian
Published: August 2021

DNA origami nanostructures (DONs) are promising substrates for the single-molecule investigation of biomolecular reactions and dynamics by in situ atomic force microscopy (AFM). For this, they are typically immobilized on mica substrates by adding millimolar concentrations of Mg2+ ions to the sample solution, which enable the adsorption of the negatively charged DONs at the like-charged mica surface. These non-physiological Mg2+ concentrations, however, present a serious limitation in such experiments as they may interfere with the reactions and processes under investigation. Therefore, we here evaluate three approaches to efficiently immobilize DONs at mica surfaces under essentially Mg2+-free conditions. These approaches rely on the pre-adsorption of different multivalent cations, i.e., Ni2+, poly-l-lysine (PLL), and spermidine (Spdn). DON adsorption is studied in phosphate-buffered saline (PBS) and pure water. In general, Ni2+ shows the worst performance with heavily deformed DONs. For 2D DON triangles, adsorption at PLL- and in particular Spdn-modified mica may outperform even Mg2+-mediated adsorption in terms of surface coverage, depending on the employed solution. For 3D six-helix bundles, less pronounced differences between the individual strategies are observed. Our results provide some general guidance for the immobilization of DONs at mica surfaces under Mg2+-free conditions and may aid future in situ AFM studies.
Severe Acute Respiratory Syndrome Coronavirus-2 Inactivation Activity of the Polyphenol-Rich Tea Leaf Extract with Concentrated Theaflavins and Other Virucidal Catechins
Authors: Takeda Yohei , Tamura Kyohei , Jamsransuren Dulamjav , Matsuda Sachiko , Ogawa Haruko
Published: August 2021

Since severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is producing a large number of infections and deaths globally, the development of supportive and auxiliary treatments is attracting increasing attention. Here, we evaluated SARS-CoV-2-inactivation activity of the polyphenol-rich tea leaf extract TY-1 containing concentrated theaflavins and other virucidal catechins. The TY-1 was mixed with SARS-CoV-2 solution, and its virucidal activity was evaluated. To evaluate the inhibition activity of TY-1 in SARS-CoV-2 infection, TY-1 was co-added with SARS-CoV-2 into cell culture media. After 1 h of incubation, the cell culture medium was replaced, and the cells were further incubated in the absence of TY-1. The viral titers were then evaluated. To evaluate the impacts of TY-1 on viral proteins and genome, TY-1-treated SARS-CoV-2 structural proteins and viral RNA were analyzed using western blotting and real-time RT-PCR, respectively. TY-1 showed time- and concentration-dependent virucidal activity. TY-1 inhibited SARS-CoV-2 infection of cells. The results of western blotting and real-time RT-PCR suggested that TY-1 induced structural change in the S2 subunit of the S protein and viral genome destruction, respectively. Our findings provided basic insights in vitro into the possible value of TY-1 as a virucidal agent, which could enhance the current SARS-CoV-2 control measures.
Lignans from Machilus thunbergii as Thymic Stromal Lymphopoietin Inhibitors
Authors: Shin Hyeji , Han Yoo Kyong , Byun Youngjoo , Jeon Young Ho , Lee Ki Yong
Published: August 2021

Thymic stromal lymphopoietin (TSLP) plays an important role in the pathophysiology of various allergic diseases that are mediated by T helper cell type-2 (Th2) responses, including asthma and atopic dermatitis. The primary focus of this study was the identification of potent inhibitors of the TSLP signaling pathway for potential therapeutic use. The 80% methanol extract of Machilus thunbergii bark significantly inhibited the signal transducer and activator of transcription 5 (STAT5) phosphorylation in human mast cell (HMC)-1 cells. Through activity-guided isolation, three lignans (1–3) were obtained and identified as (+)-galbelgin (1), meso-dihydroguaiaretic acid (2), and machilin A (3). Among them, two lignans (1 and 2) significantly inhibited STAT5 phosphorylation and TSLP/TSLPR interaction, as determined by ELISA. Our results indicated that lignans isolated from M. thunbergii are a promising resource for the treatment of allergic diseases.
The Nephroprotective Effect of Zizyphus lotus L. (Desf.) Fruits in a Gentamicin-Induced Acute Kidney Injury Model in Rats: A Biochemical and Histopathological Investigation
Authors: Bencheikh Noureddine , Bouhrim Mohamed , Kharchoufa Loubna , Al Kamaly Omkulthom Mohamed , Mechchate Hamza , Es-safi Imane , Dahmani Ahmed , Ouahhoud Sabir , El Assri Soufiane , Eto Bruno , Bnouham Mohamed , Choukri Mohammed , Elachouri Mostafa
Published: August 2021

Zizyphus lotus L. (Desf.) (Z. lotus) is a medicinal plant largely distributed all over the Mediterranean basin and is traditionally used by Moroccan people to treat many illnesses, including kidney failure. The nephrotoxicity of gentamicin (GM) has been well documented in humans and animals, although the preventive strategies against it remain to be studied. In this investigation, we explore whether the extract of Zizyphus lotus L. (Desf.) Fruit (ZLF) exhibits a protective effect against renal damage produced by GM. Indeed, twenty-four Wistar rats were separated into four equal groups of six each (♂/♀ = 1). The control group was treated orally with distilled water (10 mL/kg); the GM treated group received distilled water (10 mL/kg) and an intraperitoneal injection of GM (80 mg/kg) 3 h after; and the treated groups received ZLF extract orally at the doses 200 or 400 mg/kg and injected intraperitoneally with the GM. All treatments were given daily for 14 days. At the end of the experiment, the biochemical parameters and the histological observation related the kidney function was explored. ZLF treatment has significantly attenuated the nephrotoxicity induced by the GM. This effect was indicated by its capacity to decrease significantly the serum creatinine, uric acid, urea, alkaline phosphatase, gamma-glutamyl-transpeptidase, albumin, calcium, sodium amounts, water intake, urinary volume, and relative kidney weight. In addition, this effect was also shown by the increase in the creatinine clearance, urinary creatinine, uric acid, and urea levels, weight gain, compared to the rats treated only with the GM. The hemostasis of oxidants/antioxidants has been significantly improved with the treatment of ZLF extract, which was shown by a significant reduction in malondialdehydes levels. Histopathological analysis of renal tissue was correlated with biochemical observation. Chemical analysis by HPLC-DAD showed that the aqueous extract of ZLF is rich in phenolic compounds such as 3-hydroxycinnamic acid, catechin, ferulic acid, gallic acid, hydroxytyrosol, naringenin, p- coumaric Acid, quercetin, rutin, and vanillic acid. In conclusion, ZLF extract improved the nephrotoxicity induced by GM, through the improvement of the biochemical and histological parameters and thus validates its ethnomedicinal use.
Innovative Combination of Dispersive Solid Phase Extraction Followed by NIR-Detection and Multivariate Data Analysis for Prediction of Total Polyphenolic Content
Authors: Kappacher Christoph , Neurauter Markus , Rainer Matthias , Bonn Günther K. , Huck Christian W.
Published: August 2021

Recently polyphenols attracted great interest in the field of food and nutrition as well as in the pharmaceutical and cosmetics industries due to their health benefits through antioxidative behavior in the human body. However, because of the high number of compounds characterized as phenols and their structural diversity, quantification of polyphenols turns out to be a highly complex task. Although, a wide variety of analytical methods are used for the determination of total polyphenolic content, they are all found to be lacking in a variety of different tasks, such as their limits of detection and quantification, repeatability, accuracy and specificity. For this reason, a novel approach combining the advantages of solid phase purification, near infrared analysis and multivariate data analysis was investigated for the prediction of total polyphenolic content, suitable for a wide range of sample matrices. Dispersive solid phase extraction was performed and optimized using polyvinylpyrrolidone as sorbent, known to selectively bind polyphenols. Near-infrared detection of adsorbed polyphenols was carried out subsequently. Furthermore, the method was in-house validated, examining selectivity, repeatability and accuracy, working range, as well as multivariate limit of detection and limit of quantification, comparing it with two routinely used methods—namely, Folin–Ciocalteu photometric assay and Löwenthal titration. The novel established method was applied for the prediction of total polyphenolic content in tea and wine samples.
Characterization of Magnesium-Polylactic Acid Films Casted on Different Substrates and Doped with Diverse Amounts of CTAB
Authors: Hierro-Oliva Margarita , Luque-Agudo Verónica , Gallardo-Moreno Amparo M. , González-Martín M. Luisa
Published: August 2021

Polylactic acid (PLA) is a good candidate for the manufacture of polymeric biodegradable biomaterials. The inclusion of metallic particles and surfactants solves its mechanical limitations and improves its wettability, respectively. In this work, cetyltrimethylammonium bromide (CTAB) and magnesium particles have been incorporated into PLA films to evaluate the changes produced in the polymeric matrix cast on glass and silicone substrates. For this purpose, the surface of the films has been characterized by means of contact angle measurements and ToF-SIMS. Depth profiles and SEM images of the cross sections of the films have also been obtained to study their morphology. The results show that the CTAB in the polymer matrix with and without magnesium improves the wettability of the films, making them more suitable for cell adhesion. The higher the hydrophilicity, the higher the surfactant concentration. The depth profiles show, for the first time, that, depending on the surfactant concentration and the presence of Mg, there is a layer-like distribution near the surface where, in addition to the CTAB + PLA mixture, a surfactant exclusion zone can be seen. This new structure could be relevant in in vitro/in vivo situations when the degradation processes remove the film components in a sequential form.
Fluorescent Immunoassays for Detection and Quantification of Cardiac Troponin I: A Short Review
Authors: Radha Remya , Shahzadi Syeda Kiran , Al-Sayah Mohammad Hussein
Published: August 2021

Cardiovascular diseases are considered one of the major causes of human death globally. Myocardial infarction (MI), characterized by a diminished flow of blood to the heart, presents the highest rate of morbidity and mortality among all other cardiovascular diseases. These fatal effects have triggered the need for early diagnosis of appropriate biomarkers so that countermeasures can be taken. Cardiac troponin, the central key element of muscle regulation and contraction, is the most specific biomarker for cardiac injury and is considered the “gold standard”. Due to its high specificity, the measurement of cardiac troponin levels has become the predominant indicator of MI. Various forms of diagnostic methods have been developed so far, including chemiluminescence, fluorescence immunoassay, enzyme-linked immunosorbent assay, surface plasmon resonance, electrical detection, and colorimetric protein assays. However, fluorescence-based immunoassays are considered fast, accurate and most sensitive of all in the determination of cardiac troponins post-MI. This review represents the strategies, methods and levels of detection involved in the reported fluorescence-based immunoassays for the detection of cardiac troponin I.
The Impacts of Different Biological Treatments on the Transformation of Explosives Waste Contaminated Sludge
Authors: Aburto-Medina Arturo , Shahsavari Esmaeil , Taha Mohamed , Bates Andrew , Van Ieperen Leon , Ball Andrew S.
Published: August 2021

The dinitrotoluene isomers 2,4 and 2,6-dinitrotoluene (DNT) represent highly toxic, mutagenic, and carcinogenic compounds used in explosive manufacturing and in commercial production of polyurethane foam. Bioremediation, the use of microbes to degrade residual DNT in industry wastewaters, represents a promising, low cost and environmentally friendly alternative technology to landfilling. In the present study, the effect of different bioremediation strategies on the degradation of DNT in a microcosm-based study was evaluated. Biostimulation of the indigenous microbial community with sulphur phosphate (2.3 g/kg sludge) enhanced DNT transformation (82% transformation, from 300 g/L at Day 0 to 55 g/L in week 6) compared to natural attenuation over the same period at 25 °C. The indigenous microbial activity was found to be capable of transforming the contaminant, with around 70% transformation of DNT occurring over the microcosm study. 16S rDNA sequence analysis revealed that while the original bacterial community was dominated by Gammaproteobacteria (30%), the addition of sulphur phosphate significantly increased the abundance of Betaproteobacteria by the end of the biostimulation treatment, with the bacterial community dominated by Burkholderia (46%) followed by Rhodanobacter, Acidovorax and Pseudomonas. In summary, the results suggest biostimulation as a treatment choice for the remediation of dinitrotoluenes and explosives waste.
Visible Light-Driven GO/TiO2-CA Nano-Photocatalytic Membranes: Assessment of Photocatalytic Response, Antifouling Character and Self-Cleaning Ability
Authors: Khurram Rooha , Javed Aroosa , Ke Ruihua , Lena Cheng , Wang Zhan
Published: August 2021

Photocatalysis and membrane technology in a single unit is an ideal strategy for the development of wastewater treatment systems. In this work, novel GO (x wt%)/TiO2-CA hybrid membranes have been synthesized via a facile non-solvent induced phase inversion technique. The strategy aimed to address the following dilemmas: (1) Effective utilization of visible light and minimize e−/h+ recombination; (2) Enhanced separation capability and superior anti-fouling and self-cleaning ability. The experimental results reveal that the integration of nano-composite (GO/TiO2) boosts the membrane properties when compared to pristine CA and single photocatalyst employed membrane (GO-CA and TiO2-CA). The effect of GO content on the properties of the photocatalytic membrane has been determined by utilizing three different ratios of GO, viz. 0.5 wt%, 1 wt%, and 2 wt% designated as NC(1)-CA, NC(2)-CA, and NC(3)-CA, respectively. Amongst them, NC(3)-CA membrane showed state-of-the-art performance with an elevated photocatalytic response (four times higher than pristine CA membrane) toward methyl orange. Moreover, the water flux of NC(3)-CA membrane is 613 L/m2h, approximately three times higher than bare CA membrane (297 L/m2h), while keeping the MO rejection high (96.6%). Besides, fouling experiments presented the lowest total and fouling resistance ratios and a higher flux recovery ratio (91.78%) for the NC(3)-CA membrane, which endows the membrane with higher anti-fouling and self-cleaning properties. Thus, NC(3)-CA membrane outperforms the other as synthesized membranes in terms of separation efficiency, visible light photo-degradation of pollutant, anti-fouling and self-cleaning ability. Therefore, NC(3)-CA membrane is considered as the next generation membrane for exhibiting great potential for the wastewater treatment applications.
Evaluation of Inkjet-Printed Reduced and Functionalized Water-Dispersible Graphene Oxide and Graphene on Polymer Substrate—Application to Printed Temperature Sensors
Authors: Barmpakos Dimitris , Belessi Vassiliki , Schelwald Rayner , Kaltsas Grigoris
Published: August 2021

The present work reports on the detailed electro-thermal evaluation of a highly water dispersible, functionalized reduced graphene oxide (f-rGO) using inkjet printing technology. Aiming in the development of printed electronic devices, a flexible polyimide substrate was used for the structures’ formation. A direct comparison between the f-rGO ink dispersion and a commercial graphene inkjet ink is also presented. Extensive droplet formation analysis was performed in order to evaluate the repeatable and reliable jetting from an inkjet printer under study. Electrical characterization was conducted and the electrical characteristics were assessed under different temperatures, showing that the water dispersion of the f-rGO is an excellent candidate for application in printed thermal sensors and microheaters. It was observed that the proposed f-rGO ink presents a tenfold increased temperature coefficient of resistance compared to the commercial graphene ink (G). A successful direct interconnection implementation of both materials with commercial Ag-nanoparticle ink lines was also demonstrated, thus allowing the efficient electrical interfacing of the printed structures. The investigated ink can be complementary utilized for developing fully printed devices with various characteristics, all on flexible substrates with cost-effective, few-step processes.
Three-Dimensional Au/Ag Nanoparticle/Crossed Carbon Nanotube SERS Substrate for the Detection of Mixed Toxic Molecules
Authors: Wei Haonan , Peng Zhisheng , Yang Cheng , Tian Yuan , Sun Lianfeng , Wang Gongtang , Liu Mei
Published: August 2021

Research on engineering “hotspots” in the field of surface-enhanced Raman scattering (SERS) is at the forefront of contributing to the best sensing indicators. Currently, there is still an urgent need to design a high-strength and large-scale electric field distribution method in order to obtain an ideal SERS sensor. Here, we designed a three-dimensional (3D) Au/Ag nanoparticle (NP)/crossed carbon nanotube film SERS substrate. The proposed structure formed by the simple preparation process can perfectly coordinate the interaction between the SERS substrates, lasers, and molecules. The denser “hotspots” can be induced and then distributed in holes enclosed by Au/AgNPs and the gaps between them. This process was verified by numerical simulations. The experimental results show that the proposed SERS substrate possesses an excellent sensitivity of 10−12 M (rhodamine 6G (R6G)), an enhancement factor of 1.60 × 109, and a good signal reproducibility (the relative standard deviation is ~6.03%). We further use a Au/AgNP/crossed CNT substrate to detect complex solutions composed of toxic molecules, which shows that our proposed SERS substrate has a wide range of application potentials, especially in food safety.
Soy Isoflavones Intake and Obesity in Chinese Adults: A Cross-Sectional Study in Shanghai, China
Authors: Zhu Junjie , Zhao Qi , Qiu Yun , Zhang Yue , Cui Shuheng , Yu Yuting , Chen Bo , Zhu Meiying , Wang Na , Liu Xing , Jiang Yonggen , Xu Wanghong , Zhao Genming
Published: August 2021

This study was designed to examine the association of soy isoflavones (SI) intake with different body measurements indicative of obesity in Chinese adults of Shanghai, a population consuming foods rich in SI. This study used baseline data from the Shanghai Gaofeng cohort study. SI intake was measured by using a self-reported food frequency questionnaire (FFQ). A restricted cubic spline (RCS) was performed to examine the possible nonlinear relationship of SI intake with obesity. A logistic regression model was applied to estimate the odds ratios (OR) and 95% confidence interval (CI). Compared with the lowest tertile group of SI intake, the highest tertile group had a lower prevalence of obesity and central obesity. The OR for overall obesity was 0.91 (95% CI: 0.85, 0.98) in the highest versus the lowest SI tertile group; the associations differed by sex and menopausal status. A negative association was also observed between SI intake and central obesity, and a significant modifying effect of sex was found on the association. No significant interactions were observed between SI intake and physical activity (PA) levels. Our results suggest that Chinese adults with higher dietary intake of SI may be less likely to be obese, particularly for postmenopausal women.
Comparison of Healthiness, Labelling, and Price between Private and Branded Label Packaged Foods in New Zealand (2015–2019)
Authors: Castro Teresa , Mackay Sally , Young Leanne , Ni Mhurchu Cliona , Shaw Grace , Tawfiq Essa , Eyles Helen
Published: August 2021

We aimed to compare New Zealand private label (PL) and branded label (BL) packaged food products in relation to their current (2019) healthiness (sodium and sugar contents, and estimated Health Star Rating (HSR) score), display of the voluntary HSR nutrition label on the package, and price. Healthiness and HSR display of products were also explored over time (2015 to 2019). Data were obtained from Nutritrack, a brand-specific food composition database. Means and proportions were compared using Student t-tests and Pearson chi-square tests, respectively. Changes over time were assessed using linear regression and chi-square tests for trends (Mantel–Haenzel tests). Altogether, 4266 PL and 19,318 BL products across 21 food categories were included. Overall, PL products in 2019 had a significantly lower mean sodium content and price, a higher proportion of products with estimated HSR ≥ 3.5/5 (48.9% vs. 38.5%) and were more likely to display the HSR on the pack compared with BL products (92.4% vs. 17.2%, respectively). However, for most food categories, no significant difference was found in mean sodium or sugar content between PL and BL products. In the period 2015–2019, there were no consistent changes in estimated HSR score, sodium or sugar contents of PL or BL products, but there was an increase in the proportion of both PL and BL products displaying HSR labels. In most food categories, there were PL options available which were similar in nutritional composition, more likely to be labelled with the HSR, and lower in cost than their branded counterparts.
Punicalagin in Cancer Prevention—Via Signaling Pathways Targeting
Authors: Berdowska Izabela , Matusiewicz Małgorzata , Fecka Izabela
Published: August 2021

The extract of pomegranate (Punica granatum) has been applied in medicine since ancient times due to its broad-spectrum health-beneficial properties. It is a rich source of hydrolyzable tannins and anthocyanins, exhibiting strong antioxidative, anti-inflammatory, and antineoplastic properties. Anticancer activities of pomegranate with reference to modulated signaling pathways in various cancer diseases have been recently reviewed. However, less is known about punicalagin (Pug), a prevailing compound in pomegranate, seemingly responsible for its most beneficial properties. In this review, the newest data derived from recent scientific reports addressing Pug impact on neoplastic cells are summarized and discussed. Its attenuating effect on signaling circuits promoting cancer growth and invasion is depicted. The Pug-induced redirection of signal-transduction pathways from survival and proliferation into cell-cycle arrest, apoptosis, senescence, and autophagy (thus compromising neoplastic progression) is delineated. Considerations presented in this review are based mainly on data obtained from in vitro cell line models and concern the influence of Pug on human cervical, ovarian, breast, lung, thyroid, colorectal, central nervous system, bone, as well as other cancer types.
Assessing the Nutrient Adequacy in China’s Food Supply from 1965 to 2018
Authors: Liu Ao , Han Aixi , Chai Li
Published: August 2021

Nutritional intake has important impacts on human health. A sufficient supply of nutrients is required to ensure high-level nutrition in a population. Assessment of nutrient supply adequacy can help to develop evidence-based policies and thereby promote public health. This study estimates the supply adequacy of nutrients in China’s food system from 1965 to 2018 at the national level, aiming to reveal whether the supply of nutrients meets the demand. The results show that the nutrient supply in China’s food system has experienced a sharp increase in the past five decades, and the deficiency in nutrient supply has been greatly mitigated. Although most nutrients such as potassium are already sufficiently supplied in China’s current food system, some nutrients, especially calcium and zinc, still need a further enlarged supply to improve the nutrition condition of the Chinese population. Besides encouraging a healthy diet, supply-side regulation, e.g., fortification and enrichment, is also needed to improve nutrient availability. This study helps people better understand the development and current situation of nutrient adequacy in China’s food supply, thereby providing information and implications for policymakers.
The Effect of Saffron Supplementation on Blood Pressure in Adults: A Systematic Review and Dose-Response Meta-Analysis of Randomized Controlled Trials
Authors: Setayesh Leila , Ashtary-Larky Damoon , Clark Cain C. T. , Rezaei Kelishadi Mahnaz , Khalili Pardis , Bagheri Reza , Asbaghi Omid , Suzuki Katsuhiko
Published: August 2021

Background: The favorable influences of saffron supplementation on metabolic diseases have previously been shown. We aimed to assess the effects of saffron supplementation on blood pressure in adults. Methods: A systematic search was performed in Scopus, Embase, and the Cochrane library databases to find randomized controlled trials (RCTs) related to the effect of saffron supplementation on blood pressure in adults up to March 2021. The primary search yielded 182 publications, of which eight RCTs were eligible. Results: Our results showed that saffron supplementation resulted in a significant decrease in systolic blood pressure (weighted mean difference (WMD): −0.65 mmHg; 95% CI: −1.12 to −0.18, p = 0.006) and diastolic blood pressure (DBP) (WMD: −1.23 mmHg; 95% CI: −1.64 to −0.81, p < 0.001). Moreover, saffron supplementation reduced DBP in a non-linear fashion, based on duration (r = −2.45, p-nonlinearity = 0.008). Conclusions: Saffron supplementation may significantly improve both systolic and diastolic blood pressure in adults. It should be noted that the hypotensive effects of saffron supplementation were small and may not reach clinical importance.
Mosquitoes Know No Borders: Surveillance of Potential Introduction of Aedes Species in Southern Québec, Canada
Authors: Lowe Anne-Marie , Forest-Bérard Karl , Trudel Richard , Lo Ernest , Gamache Philippe , Tandonnet Matthieu , Kotchi Serge-Olivier , Leighton Patrick , Dibernardo Antonia , Lindsay Robbin , Ludwig Antoinette
Published: August 2021

Current climatic conditions limit the distribution of Aedes (Stegomyia) albopictus (Skuse, Diptera: Culicidae) in the north, but predictive climate models suggest this species could establish itself in southern Canada by 2040. A vector of chikungunya, dengue, yellow fever, Zika and West Nile viruses, the Ae. Albopictus has been detected in Windsor, Ontario since 2016. Given the potential public health implications, and knowing that Aedes spp. can easily be introduced by ground transportation, this study aimed to determine if specimens could be detected, using an adequate methodology, in southern Québec. Mosquitoes were sampled in 2016 and 2017 along the main roads connecting Canada and the U.S., using Biogent traps (Sentinel-2, Gravide Aedes traps) and ovitraps. Overall, 24 mosquito spp. were captured, excluding Ae. Albopictus, but detecting one Aedes (Stegomyia) aegypti (Skuse) specimen (laid eggs). The most frequent species among captured adults were Ochlerotatus triseriatus, Culex pipiens complex, and Ochlerotatus japonicus (31.0%, 26.0%, and 17.3%, respectively). The present study adds to the increasing number of studies reporting on the range expansions of these mosquito species, and suggests that ongoing monitoring, using multiple capture techniques targeting a wide range of species, may provide useful information to public health with respect to the growing risk of emerging mosquito-borne diseases in southern Canada.
Bioavailability and Antidiabetic Activity of Gliclazide-Loaded Cubosomal Nanoparticles
Authors: Nasr Mohamed , Almawash Saud , Al Saqr Ahmed , Bazeed Alaa Y. , Saber Sameh , Elagamy Heba I.
Published: August 2021

In this study, gliclazide-loaded cubosomal particles were prepared for improving the oral bioavailability and antidiabetic activity of gliclazide. Four formulations of gliclazide-loaded cubosomal nanoparticles dispersions were prepared by the emulsification method using four different concentrations of glyceryl monooleate (GMO) and poloxamer 407 (P407) as the stabilizer. The prepared formulations were in vitro and in vivo evaluated. In vitro, the prepared gliclazide-loaded cubosomal dispersions exhibited disaggregated regular poly-angular particles with a nanometer-sized particle range from 220.60 ± 1.39 to 234.00 ± 2.90 nm and entrapped 73.84 ± 3.03 to 88.81 ± 0.94 of gliclazide. In vitro gliclazide release from cubosomal nanoparticles revealed an initially higher drug release during the first 2 h in acidic pH medium; subsequently, a comparatively higher drug release in alkaline medium relative to gliclazide suspension was observed. An in vivo absorption study in rats revealed a two-fold increase in the bioavailability of gliclazide cubosomal formulation relative to plain gliclazide suspension. Moreover, the study of in vivo hypoglycemic activity indicated that a higher percentage reduction in glucose level was observed after the administration of gliclazide cubosomal nanoparticles to rats. In conclusion, gliclazide-loaded cubosomal nanoparticles could be a promising delivery system for improving the oral absorption and antidiabetic activity of gliclazide.
Pharmacological and Biological Study of Microencapsulated Probucol-Secondary Bile Acid in a Diseased Mouse Model
Authors: Wagle Susbin , Kovacevic Bozica , Ionescu Corina , Walker Daniel , Jones Melissa , Carey Louise , Takechi Ryusuke , Mikov Momir , Mooranian Armin , Al-Salami Hani
Published: August 2021

Probucol (PB) is a highly lipophilic drug with potential protective effects on pancreatic β-cells from inflammation and oxidation. PB has poor bioavailability and solubility, and despite many attempts, significant improvement in antidiabetic effects or absorption has yet to be discovered. Recently, the role of bile acids has been established in significant drug formulation stabilisation effects and as cell-penetrating agents. Promising results in pharmaceutical formulation studies on drug stability and release patterns when lithocholic acid (LCA) is conjugated with PB and sodium alginate (SA) have been demonstrated. Thus, this study aimed to develop and characterise PB microcapsules incorporating LCA and examine the biological effects of the microcapsules in vitro and in vivo. PB/LCA microcapsules were prepared using an encapsulation method, ionic gelation vibrational jet flow technology. LCA incorporation in PB microcapsules showed positive effects on β-cells with improved insulin release, antioxidant activity, and PB intracellular uptake. Diabetic mice gavaged LCA-PB microcapsules showed a significant reduction in diabetes signs and symptoms, better survival rate, reduced blood glucose levels, and pro-inflammatory cytokines, with an increase PB level in blood and tissues suggesting a potential therapy for treating diabetes mellitus.
A Review of Ocular Drug Delivery Platforms and Drugs for Infectious and Noninfectious Uveitis: The Past, Present, and Future
Authors: Conrady Christopher D. , Yeh Steven
Published: August 2021

Uveitis refers to a broad group of inflammatory disorders of the eye that often require medical and surgical management to improve or stabilize vision and prevent vision-threatening pathological changes to the eye. Drug delivery to the eye to combat inflammation and subsequent complications from uveitic conditions is complex as there are multiple barriers to absorption limiting availability of the needed drug in the affected tissues. As such, there has been substantial interest in developing new drugs and drug delivery platforms to help reduce intraocular inflammation and its complications. In this review, we discuss the challenges of drug delivery, novel technologies recently approved for uveitis patient care and promising drug delivery platforms for uveitis and sequelae of ocular inflammation.
Induction of Catharanthus roseus Secondary Metabolites When Calotropis procera Was Used as Bio-Stimulant
Authors: Abeed Amany H. A. , Ali Mohammed , Ali Esmat F. , Majrashi Ali , Eissa Mamdouh A.
Published: August 2021

Available information associated with Calotropis procera posted its phytotoxic effect as bio-herbicide scarce works studied its stimulatory/nutritive effect. A pot experiment was performed to assess the validity of using Calotropis procera (C. procera) leaves extract as a bio-stimulant for the growth and quality of a medicinal plant Catharanthus roseus (C. roseus) evaluated by some physio-biochemical indices. Different types of C. procera leaves extracts (CLEs) (methanolic, cold water and autoclaved water extracts) were delivered by two different modes of application. The results revealed that application of CLEs as irrigation or foliar spraying caused a stimulation effect on C. roseus plant. Root and shoot length, dry and fresh weight were significantly improved due to CLEs applications. C. roseus bioactive molecules such as anthocyanins, phenolics, flavonoids, alkaloids, ascorbic acid, reduced glutathione and α-tocopherol were abundance increased significantly with CLEs applications. Reactive oxygen species (ROS) decreased explaining the involvement of CLEs in induction of antioxidant enzymes catalase, ascorbate peroxidase, polyphenol oxidase, guaiacol peroxidase and glutathione-S-transferase for modifying cell oxidative status witnessed by lower lipid peroxidation that kept below the untreated plants’ baseline reflected the improvement of growth and quality rather than phytotoxic effect. The promotion of wholesome-promoting secondary metabolites by CLEs was closely correlated to elevated phenylalanineammonialyase activity. The comparable efficient effect induced by all treatments might be judged by the relation between C. procera phytochemicals and C. roseus metabolism (donor-receiver relation). It is concluded that application of CLEs can be a promising approach for improving the yield and quality of plants despite using polluting fertilizers. The current investigation may provide a matrix for coming studies to seek illustration of numerous plants’ response to C. procera extracts.
Effects of Climate Change on Vegetation Cover in the Oued Lahdar Watershed. Northeastern Morocco
Authors: Khalis Hind , Sadiki Abdelhamid , Jawhari Fatimazahra , Mesrar Haytam , Azab Ehab , Gobouri Adil A. , Adnan Muhammad , Bourhia Mohammed
Published: August 2021

Episodes of drought that Morocco experienced in the years 1984–1986, 1993–1995, and 1997–2000 had repercussions that were felt many years later and continue to pose serious problems for environmentalists, as some of the affected lands have become practically deserted. These problems acted on the socio-economic conditions and created severe constraints for the development of the country. This work was conducted to study and identify changes that occurred in vegetation cover in the Oued Lahdar watershed (Rif, Morocco) between 1984 and 2017 using Land Surface Temperature (LST), Normalized Difference Vegetation Index (NDVI), Landsat TM 5, and Landsat OLI 8. The LST had significantly increased overall from 1984 to 2017, where it moved from a mean value of 29.4 °C in 1984 to 40.4 °C in 2007 and then reduced slightly to 37.9 °C in 2017. The vegetation cover index for the study area indicates that in 1984, fully vegetated areas represented 94.3% before deteriorating to 35.4% in 2007 and recovering in 2017 to 54.3%. While bare soil, which previously constituted 5.7%, reached a very high value of 64.6% in 2007 and then decreased to 47.7%. This study contributes towards society as it provides interesting data about the consequences of climate change in the area studied as well as potential protective strategies to protect vegetation cover.
Automated Grapevine Cultivar Identification via Leaf Imaging and Deep Convolutional Neural Networks: A Proof-of-Concept Study Employing Primary Iranian Varieties
Authors: Nasiri Amin , Taheri-Garavand Amin , Fanourakis Dimitrios , Zhang Yu-Dong , Nikoloudakis Nikolaos
Published: August 2021

Extending over millennia, grapevine cultivation encompasses several thousand cultivars. Cultivar (cultivated variety) identification is traditionally dealt by ampelography, requiring repeated observations by experts along the growth cycle of fruiting plants. For on-time evaluations, molecular genetics have been successfully performed, though in many instances, they are limited by the lack of referable data or the cost element. This paper presents a convolutional neural network (CNN) framework for automatic identification of grapevine cultivar by using leaf images in the visible spectrum (400–700 nm). The VGG16 architecture was modified by a global average pooling layer, dense layers, a batch normalization layer, and a dropout layer. Distinguishing the intricate visual features of diverse grapevine varieties, and recognizing them according to these features was conceivable by the obtained model. A five-fold cross-validation was performed to evaluate the uncertainty and predictive efficiency of the CNN model. The modified deep learning model was able to recognize different grapevine varieties with an average classification accuracy of over 99%. The obtained model offers a rapid, low-cost and high-throughput grapevine cultivar identification. The ambition of the obtained tool is not to substitute but complement ampelography and quantitative genetics, and in this way, assist cultivar identification services.
Molecular Cloning and Expression Profile of Class E Genes Related to Sepal Development in Nelumbo nucifera
Authors: Liu Zhuoxing , Zhang Dasheng , Zhang Weiwei , Xiong Lei , Liu Qingqing , Liu Fengluan , Li Hanchun , An Xiangjie , Cui Lijie , Tian Daike
Published: August 2021

The lotus (Nelumbo Adans.) is an important aquatic plant with ornamental, medicinal and edible values and cultural connotations. It has single-, semi-double-, double- and thousand-petalled types of flower shape and is an ideal material for developmental research of flower doubling. The lotus is a basal eudicot species without a morphological difference between the sepals and petals and occupies a critical phylogenetic position in flowering plants. In order to investigate the genetic relationship between the sepals and petals in the lotus, the class E genes which affect sepal formation were focused on and analyzed. Here, SEPALLATA 1(NnSEP1) and its homologous genes AGAMOUS-LIKE MADS-BOXAGL9 (NnAGL9) and MADS-BOX TRANSCRIPTION FACTOR 6-like (NnMADS6-like) of the class E gene family were isolated from the flower buds of the Asian lotus (Nelumbo nucifera Gaertn.). The protein structure, subcellular localization and expression patterns of these three genes were investigated. All three genes were verified to locate in the nucleus and had typical MADS-box characteristics. NnSEP1 and NnMADS6-like were specifically expressed in the sepals, while NnAGL9 was highly expressed in the petals, suggesting that different developmental mechanisms exist in the formation of the sepals and petals in the lotus. The significant functional differences between NnSEP1, NnMADS6-like and NnAGL9 were also confirmed by a yeast two-hybrid assay. These results expand our knowledge on the class E gene family in sepal formation and will benefit fundamental research on the development of floral organs in Nelumbo.
Evaluation of Preemergent Herbicides for Chloris virgata Control in Mungbean
Authors: Mahajan Gulshan , Chauhan Bhagirath S.
Published: August 2021

Chloris virgata is a problematic weed in mungbean crops due to its high seed production, resistance to glyphosate and high dispersal ability. Pot and field experiments were conducted in 2020 and 2021 to evaluate a range of preemergent (PRE) herbicides for C. virgata control in mungbean. In the field and pot studies, isoxaflutole 75 g ai ha−1 caused crop injury, and in the field experiment, it reduced mungbean yield by 61% compared with the best treatment (pyroxasulfone 100 g ai ha−1). In the field and pot experiments, dimethenamid-P 720 g ai ha−1, pyroxasulfone 100 g ai ha−1 and S-metolachlor 1400 g ai ha−1 provided >88% control of C. virgata (for reduced biomass) and in the field experiment, these herbicides resulted in improved yield by 230%, 270% and 170%, respectively, compared with nontreated control (250 kg ha−1). Similarly, pendimethalin 1000 g ai ha−1 and trifluralin 600 g ai ha−1 provided >89% control (biomass) of C. virgata, and in the field experiment, these resulted in improved yields of 230% and 160%, respectively, compared with the nontreated control. PRE herbicides such as diuron 750 g ai ha−1, linuron 1100 g ai ha−1, metribuzin 360 g ha−1, terbuthylazine 750 g ai ha−1, imazapic 48 g ai ha−1 and imazethapyr 70 g ha−1 although did not cause crop injury; however, these herbicides did not control C. virgata. Flumioxazin 90 g ai ha−1 caused reduced biomass of C. virgata by 80% compared with the nontreated control, and in the field experiment, it resulted in improved yield by 140% compared with the nontreated control. This study suggests the potential use of herbicides, such as dimethenamid-P, pyroxasulfone and S-metolachlor in addition to pendimethalin and trifluralin, for C. virgata control in mungbean. Further studies are needed to determine the efficacy of dimethenamid-P, S-metolachlor and pyroxasulfone for controlling other troublesome weeds in mungbean.
A Comparative Study on Photo-Protective and Anti-Melanogenic Properties of Different Kadsura coccinea Extracts
Authors: Jeon Joong Suk , Kang He Mi , Park Ju Ha , Kang Jum Soon , Lee Yong Jae , Park Young Hoon , Je Byoung Il , Park Sun Young , Choi Young Whan
Published: August 2021

Kadsura coccinea (KC), a beneficial plant for human health, has been used for centuries in China, Thailand, and Korea in folk medicine and food. There is evidence supporting the biological effects of highly bioactive ingredients in KC such as lignans, triterpenoids, flavonoids, phenolic acids, steroids, and amino acids. In this study, we aimed to explore the effects, functions, and mechanisms of the extracts from KC root (KCR), stem (KCS), leaf (KCL), and fruit (KCF) in UVA and UVB-irradiated keratinocytes and α-melanocyte stimulating hormone (α-MSH)-stimulated melanocytes. First, the total polyphenol and flavonoid contents of KCR, KCS, KCL, and KCF and their radical scavenging activities were investigated. These parameters were found to be in the following order: KCL > KCR > KCS > KCF. UVA and UVB-irradiated keratinocytes were treated with KCR, KCS, KCL, and KCF, and keratinocyte viability, LDH release, intracellular ROS production, and apoptosis were examined. Our results demonstrated that KC extracts improved keratinocyte viability and reduced LDH release, intracellular ROS production, and apoptosis in the presence UVA and UVB irradiation. The overall photoprotective activity of the KC extracts was confirmed in the following order: KCL > KCR > KCS > KCF. Moreover, KC extracts significantly decreased the intracellular melanin content and tyrosinase activity in α-MSH-stimulated melanocytes. Mechanistically, KC extracts reduced the protein and mRNA expression levels of tyrosinase, tyrosinase-related protein-1 (TRP-1), and tyrosinase-related protein-2 (TRP-2) in α-MSH-stimulated melanocytes. In addition, these extracts markedly downregulated myophthalmosis-related transcription factor expression and cAMP-related binding protein phosphorylation, which is upstream of the regulation of Tyrosinase, TRP-1, and TRP-2. The overall anti-melanogenic activity of the KC extracts was established in the following order. KCL > KCR > KCS > KCF. Overall, the KC extracts exert photoprotective and anti-melanogenic effects, providing a basis for developing potential skin-whitening and photoprotective agents.
In Vitro Evaluation of Biomaterials for Vocal Fold Injection: A Systematic Review
Authors: Wan-Chiew Ng , Baki Marina Mat , Fauzi Mh Busra , Lokanathan Yogeswaran , Azman Mawaddah
Published: August 2021

Vocal fold injection is a preferred treatment in glottic insufficiency because it is relatively quick and cost-saving. However, researchers have yet to discover the ideal biomaterial with properties suitable for human vocal fold application. The current systematic review employing PRISMA guidelines summarizes and discusses the available evidence related to outcome measures used to characterize novel biomaterials in the development phase. The literature search of related articles published within January 2010 to March 2021 was conducted using Scopus, Web of Science (WoS), Google Scholar and PubMed databases. The search identified 6240 potentially relevant records, which were screened and appraised to include 15 relevant articles based on the inclusion and exclusion criteria. The current study highlights that the characterization methods were inconsistent throughout the different studies. While rheologic outcome measures (viscosity, elasticity and shear) were most widely utilized, there appear to be no target or reference values. Outcome measures such as cellular response and biodegradation should be prioritized as they could mitigate the clinical drawbacks of currently available biomaterials. The review suggests future studies to prioritize characterization of the viscoelasticity (to improve voice outcomes), inflammatory response (to reduce side effects) and biodegradation (to improve longevity) profiles of newly developed biomaterials.