bluuebunny/crossref_metadata_2025 huggingface dataset:
ON THE TIMING OF THE OLD COPPER COMPLEX IN NORTH AMERICA: A COMPARISON OF RADIOCARBON DATES FROM DIFFERENT ARCHAEOLOGICAL CONTEXTS
Authors: Pompeani David P , Steinman Byron A , Abbott Mark B , Pompeani Katherine M , Reardon William , DePasqual Seth , Mueller Robin H
Published: March 2021

The Old Copper Complex (OCC) refers to the production of heavy copper-tool technology by Archaic Native American societies in the Lake Superior region. To better define the timing of the OCC, we evaluated 53 (eight new and 45 published) radiocarbon (14C) dates associated with copper artifacts and mines. We compared these dates to six lake sediment-based chronologies of copper mining and annealing in the Michigan Copper District. 14C dates grouped by archaeological context show that cremation remains, and wood and cordage embedded in copper artifacts have ages that overlap with the timing of high lead (Pb) concentrations in lake sediment. In contrast, dates in stratigraphic association and from mines are younger than those from embedded and cremation materials, suggesting that the former groups reflect the timing of processes that occurred post-abandonment. The comparatively young dates obtained from copper mines therefore likely reflect abandonment and infill of the mines rather than active use. Excluding three anomalously young samples, the ages of embedded organic material associated with 15 OCC copper artifacts range from 8500 to 3580 cal BP, confirming that the OCC is among the oldest known metalworking societies in the world.
Vacuum magnetic fields with exact quasisymmetry near a flux surface. Part 1. Solutions near an axisymmetric surface
Authors: Sengupta Wrick , Paul Elizabeth J. , Weitzner Harold , Bhattacharjee Amitava
Published: March 2021

While several results have pointed to the existence of exactly quasisymmetric fields on a surface (Garren & Boozer, Phys. Fluids B, vol. 3, 1991, pp. 2805–2821; 2822–2834; Plunk & Helander, J. Plasma Phys., vol. 84, 2018, 905840205), we have obtained the first such solutions using a vacuum surface expansion formalism. We obtain a single nonlinear parabolic partial differential equation for a function

$\eta$

such the field strength satisfies

$B = B(\eta )$

. Closed-form solutions are obtained in cylindrical, slab and isodynamic geometries. Numerical solutions of the full nonlinear equations in general axisymmetric toroidal geometry are obtained, resulting in a class of quasihelical local vacuum equilibria near an axisymmetric surface. The analytic models provide additional insight into general features of the nonlinear solutions, such as localization of the surface perturbations on the inboard side. The local solutions thus obtained can be continued globally only for special initial surfaces.
Camera trapping reveals area of conservation significance for large and medium-sized mammals on the eastern Tibetan Plateau
Authors: Xu Yu , Wang Bin , Gu Xiaodong , Song Dazhao , Yang Biao
Published: March 2021

The eastern margin of the Tibetan Plateau, a global biodiversity hotspot, is threatened by habitat degradation. Conservation actions are required in this region, but limited knowledge of large and medium-sized mammals is hampering conservation planning. Using 149 camera traps, we surveyed large and medium-sized mammals in Xionglongxi Provincial Nature Reserve and adjacent areas, on the eastern margin of the Tibetan Plateau, during September 2016–May 2017. We obtained 5,752 independent captures of 29 large and medium-sized mammal species, including 13 that are globally threatened and 22 that are nationally threatened in China. Carnivores were especially diverse, with 16 species recorded. Of particular significance was our detection of seven felid species, including the leopard Panthera pardus and the snow leopard Panthera uncia. Our record of the Chinese mountain cat Felis bieti extends the known range of this species. We documented new upper elevation limits for eight species. There was elevational overlap between the leopard and the snow leopard, suggesting potential competition between the two species. The grey wolf Canis lupus and the leopard were the dominant predators, and the woolly hare Lepus oiostolus and several species of ungulates were the most frequently photographed prey species. The study area maintains a significant community of large and medium-sized mammals, which is more diverse than in other areas on the eastern edge of the Tibetan Plateau. We recommend the establishment of a larger nature reserve of national protection status in the region of the Xionglongxi Provincial Nature Reserve, to protect the unique subalpine and alpine ecosystems in this area.
The broader societal impacts of COVID-19 and the growing importance of capturing these in health economic analyses
Authors: Schnitzler Lena , Janssen Luca M. M. , Evers Silvia M. A. A. , Jackson Louise J. , Paulus Aggie T. G. , Roberts Tracy E. , Pokhilenko Irina
Published: March 2021

Abstract

The rapid spread of the current COVID-19 pandemic has affected societies worldwide, leading to excess mortality, long-lasting health consequences, strained healthcare systems, and additional strains and spillover effects on other sectors outside health (i.e., intersectoral costs and benefits). In this perspective piece, we demonstrate the broader societal impacts of COVID-19 on other sectors outside the health sector and the growing importance of capturing these in health economic analyses. These broader impacts include, for instance, the effects on the labor market and productivity, education, criminal justice, housing, consumption, and environment. The current pandemic highlights the importance of adopting a societal perspective to consider these broader impacts of public health issues and interventions and only omit these where it can be clearly justified as appropriate to do so. Furthermore, we explain how the COVID-19 pandemic exposed and exacerbated existing deep-rooted structural inequalities that contribute to the wider societal impacts of the pandemic.
Folic acid supplementation during oocytes maturation influences in vitro production and gene expression of bovine embryos
Authors: Gennari Verruma Carolina , Credendio Eiras Matheus , Fernandes Artur , Vila Reginaldo Aparecido , Libardi Miranda Furtado Cristiana , Silveira Ramos Ester , Barbosa Lôbo Raysildo
Published: March 2021

Embryos that are produced in vitro frequently present epigenetic modifications. However, maternal supplementation with folic acid (FA) may improve oocyte maturation and embryo development, preventing epigenetic errors in the offspring. We sought to evaluate the influence of FA supplementation during in vitro maturation of grade I (GI) and grade III (GIII) bovine oocytes on embryo production rate and the expression of IGF2 and KCNQ1OT1 genes. The oocytes were matured in vitro with different concentrations of FA (0, 10, 30 and 100 μM), followed by in vitro fertilization and embryo culture. On the seventh day (D7) of culture, embryo production was evaluated and gene expression was measured using real-time qPCR. Supplementation with 10 μM of FA did not affect embryo production for GI and GIII oocytes. Moderate supplementation (30 μM) seemed to be a positive influence, increasing embryo production for GIII (P = 0.012), while the highest dose (100 μM) reduced embryo production (P = 0.010) for GI, and IGF2 expression was not detected. In GIII, only embryos whose oocyte maturation was not supplemented with FA demonstrated detected IGF2 expression. The lowest concentration of FA (10 μM) reduced KCNQ1OT1 expression (P = 0.05) on embryos from GIII oocytes. Different FA concentrations induced different effects on bovine embryo production and gene expression that was related to oocyte quality. Despite the epigenetic effects of FA, supplementation seems to be a promising factor to improve bovine embryo production if used carefully, as concentration is an important factor, especially in oocytes with impaired quality.
Post-operative arrhythmias in patients with hypoplastic left heart syndrome and anatomic variants: incidence, type, and course
Authors: Yimer Muluneh A. , Tisma-Dupanovic Svjetlana , Malloy-Walton Lindsey , Connelly Diana , Noel-Macdonnell Janelle , O’ Brien James , Papagiannis John
Published: March 2021

Background:

Arrhythmias are common in the post-operative course of patients with hypoplastic left heart syndrome. We sought to determine the types, incidence, risk factors, and impact of arrhythmias in patients with HLHS and anatomic variants.

Methods:

We performed a retrospective chart review of 120 consecutive patients with HLHS and anatomical variants, who had single-ventricle palliation at our institution from January, 2006 to December, 2016.

Results:

A total of thirty-one patients (26%) had 37 episodes of arrhythmias over a median follow-up period of 3.5 years. Of the 37 episodes, 12 (32.4%) were ectopic atrial tachycardia, 9 (24.3%) were paroxysmal supraventricular tachycardia, 4 (10.8%) were junctional ectopic tachycardia, 5 (13.6%) were sinus node dysfunction, 3 (8.1%) were heart block, 2 (5.4%) were atrial flutter, and 2 (5.4%) were ventricular tachycardia. Twenty-four (65%) of the arrhythmias occurred at post-stage 1 surgery. Most (64.8%) of the arrhythmias were resolved. Arrhythmias that occurred at post-stage 1 surgery were more likely to resolve compared to post-stages 2 or 3 (p = 0.006). No anatomical, surgical, or clinical variables were associated with arrhythmia except for age (OR per unit decrease in age at stage 1 palliation: 1.12 (95% CI 1.003, 1.250); p = 0.0439). Arrhythmias were not associated with length of hospital stay or mortality.

Conclusion:

Arrhythmias are common in patients with HLHS and anatomic variants, with EAT and PSVT being the most common types. Arrhythmias were associated with younger age at surgery, but did not affect mortality or length of hospital stay.
Webinars reduce the environmental footprint of pediatric cardiology conferences
Authors: Duane Brett , Lyne Alexandra , Faulkner Theresa , Windram Jonathan D. , Redington Andrew N. , Saget Sophie , Tretter Justin T. , McMahon Colin J.
Published: March 2021

Background:

Webinars have recently replaced in-person medical conferences, including paediatric cardiology conferences, given the COVID-19 pandemic.

Methods:

With increasing environmental concerns, we analysed the differences between the environmental footprint of a paediatric cardiology webinar with a hypothetical conference. Travel data was collected, with assumptions made on the amount of computer use, internet use and accordingly the overall use of electricity for both forms of conference. Life Cycle Assessment methodology was used (OpenLCA and Ecovinvent v 3.7).

Results:

We showed that the theoretical environmental impact of a virtual conference is significantly less (4 tons CO2 equivalent) than the traditional international face-to-face conference (192 tons CO2 equivalent). The life cycle assessment methodology showed that resource use for a face-to-face conference lasting 2.5 days for 1374 attendees is equivalent to 400 times what an average person would use in one year, the climate change and photochemical ozone formation approximately 250 times and the eutrophication terrestrial equivalent to 225 times. However, using carbon equivalent emissions to measure environmental harm from flying is an under estimate of the potential damage, when one considers the additional production of airplane contrails. Notwithstanding this, there is a 98% reduction in climate change impact when meetings are held virtually.

Conclusions:

While the virtual conference may never completely replace the traditional in-person paediatric cardiology conference, due to networking benefits, the significant theoretical benefits to the environment highlighted in this study, warrants consideration for the virtual conference taking a more common place in sustainable academia.
How the space environment influences organisms: an astrobiological perspective and review
Authors: Prasad Binod , Richter Peter , Vadakedath Nithya , Haag Ferdinand W. M. , Strauch Sebastian M. , Mancinelli Rocco , Schwarzwälder Achim , Etcheparre Emmanuel , Gaume Nicolas , Lebert Michael
Published: March 2021

The unique environment of space is characterized by several stress factors, including intense radiation, microgravity, high vacuum and extreme temperatures, among others. These stress conditions individually or in-combination influence genetics and gene regulation and bring potential evolutionary changes in organisms that would not occur under the Earth's gravity regime (1 × g). Thus, space can be explored to support the emergence of new varieties of microbes and plants, that when selected for, can exhibit increased growth and yield, improved resistance to pathogens, enhanced tolerance to drought, low nutrient and disease, produce new metabolites and others. These properties may be more difficult to achieve using other approaches under 1 × g. This review provides an overview of the space microgravity and ionizing radiation conditions that significantly influence organisms. Changes in the genomics, physiology, phenotype, growth and metabolites of organisms in real and simulated microgravity and radiation conditions are illustrated. Results of space biological experiments show that the space environment has significant scientific, technological and commercial potential. Combined these potentials can help address the future of life on Earth, part of goal e of astrobiology.
Automatic analysis of insurance reports through deep neural networks to identify severe claims
Authors: Cohen Sabban Isaac , Lopez Olivier , Mercuzot Yann
Published: March 2021

In this paper, we develop a methodology to automatically classify claims using the information contained in text reports (redacted at their opening). From this automatic analysis, the aim is to predict if a claim is expected to be particularly severe or not. The difficulty is the rarity of such extreme claims in the database, and hence the difficulty, for classical prediction techniques like logistic regression to accurately predict the outcome. Since data is unbalanced (too few observations are associated with a positive label), we propose different rebalance algorithm to deal with this issue. We discuss the use of different embedding methodologies used to process text data, and the role of the architectures of the networks.
Prime ministerial autonomy and intra-executive conflict under semi-presidentialism
Authors: Yan Huang-Ting
Published: March 2021

This article answers why intra-executive conflict varies across semi-presidential democracies. The literature verifies that intra-executive competition tends to be higher when the president holds less power to dismiss the cabinet, coexists with a minority government, or the president’s party is not represented in the cabinet. This paper, therefore, integrates these factors to construct an index of prime ministerial autonomy, proposing that its relationship with the probability of intra-executive conflict is represented by an inverted U-shaped curve. That is, when the prime minister is subordinated to an elected president, or conversely, enjoys greater room to manoeuvre in the executive affairs of the government, the likelihood of conflict is low. In contrast, significant confrontation emerges when the president claims constitutional legitimacy to rein in the cabinet, and controls the executive to a certain degree. This study verifies hypotheses using data on seventeen semi-presidential democracies in Europe between 1990 and 2015.
Mental health problems in the general population during and after the first lockdown phase due to the SARS-Cov-2 pandemic: rapid review of multi-wave studies
Authors: Richter Dirk , Riedel-Heller Steffi , Zürcher Simeon Joel
Published: March 2021

Aims

The SARS-Cov-2 pandemic and the lockdown response are assumed to have increased mental health problems in general populations compared to pre-pandemic times. The aim of this paper is to review studies on the course of mental health problems during and after the first lockdown phase.

Methods

We conducted a rapid review of multi-wave studies in general populations with time points during and after the first lockdown phase. Repeated cross-sectional and longitudinal studies that utilised validated instruments were included. The main outcome was whether indicators of mental health problems have changed during and after the first lockdown phase. The study was registered with PROSPERO No. CRD42020218640.

Results

Twenty-three studies with 56 indicators were included in the qualitative review. Studies that reported data from pre-pandemic assessments through lockdown indicated an increase in mental health problems. During lockdown, no uniform trend could be identified. After lockdown, mental health problems decreased slightly.

Conclusions

As mental health care utilisation indicators and data on suicides do not suggest an increase in demand during the first lockdown phase, we regard the increase in mental health problems as general distress that is to be expected during a global health crisis. Several methodological, pandemic-related, response-related and health policy-related factors need to be considered when trying to gain a broader perspective on the impact of the first wave of the pandemic and the first phase of lockdown on general populations' mental health.
Ethnohistorical Archaeology and the Mythscape of the Naga in the Chiang Saen Basin, Thailand
Authors: Moonkham Piyawit
Published: March 2021

There is a northern Thai story that tells how the naga—a mythical serpent—came and destroyed the town known as Yonok (c. thirteenth century) after its ruler became immoral. Despite this divine retribution, the people of the town chose to rebuild it. Many archaeological sites indicate resettlement during this early historical period. Although many temple sites were constructed in accordance with the Buddhist cosmology, the building patterns vary from location to location and illustrate what this paper calls ‘nonconventional patterns,’ distinct from Theravada Buddhist concepts. These nonconventional patterns of temples seem to have been widely practiced in many early historical settlements, e.g., Yonok (what is now Wiang Nong Lom). Many local written documents and practices today reflect the influence of the naga myth on building construction. This paper will demonstrate that local communities in the Chiang Saen basin not only believe in the naga myth but have also applied the myth as a tool to interact with the surrounding landscapes. The myth is seen as a crucial, communicated element used by the local people to modify and construct physical landscapes, meaning Theravada Buddhist cosmology alone cannot explain the nonconventional patterns. As such, comprehending the role of the naga myth enables us to understand how local people, past and present, have perceived the myth as a source of knowledge to convey their communal spaces within larger cosmological concepts in order to maintain local customs and legitimise their social space.
Global Changes in 20‐Year, 50‐Year, and 100‐Year River Floods
Authors: Slater L. , Villarini G. , Archfield S. , Faulkner D. , Lamb R. , Khouakhi A. , Yin J.
Published: March 2021

Concepts like the 100‐year flood event can be misleading if they are not updated to reflect significant changes over time. Here, we model observed annual maximum daily streamflow using a nonstationary approach to provide the first global picture of changes in: (a) the magnitudes of the 20‐, 50‐, and 100‐year floods (i.e., flows of a given exceedance probability in each year); (b) the return periods of the 20‐, 50‐, and 100‐year floods, as assessed in 1970 (i.e., flows of a fixed magnitude); and (c) corresponding flood probabilities. Empirically, we find the 20‐/50‐year floods have mostly increased in temperate climate zones, but decreased in arid, tropical, polar, and cold zones. In contrast, 100‐year floods have mostly decreased in arid/temperate zones and exhibit mixed trends in cold zones, but results are influenced by the small number of stations with long records, and highlight the need for continued updating of hazard assessments.
The Unusual Stratospheric Arctic Winter 2019/20: Chemical Ozone Loss From Satellite Observations and TOMCAT Chemical Transport Model
Authors: Weber Mark , Arosio Carlo , Feng Wuhu , Dhomse Sandip S. , Chipperfield Martyn P. , Meier Andreas , Burrows John P. , Eichmann Kai‐Uwe , Richter Andreas , Rozanov Alexei
Published: March 2021

Satellite observations of relevant trace gases and meteorological data from ERA5 were used to describe the dynamics and chemistry of the spectacular Arctic 2019/20 winter/spring season. Exceptionally low total ozone values of slightly less than 220 DU were observed in mid‐March within an unusually large stratospheric polar vortex. Very high OClO and very low NO2 column amounts observed by GOME‐2A were indicative of unusually large active chlorine levels and significant denitrification, which likely contributed to large chemical ozone loss. Using results from the TOMCAT chemical transport model (CTM) and ozone observations from S5P/TROPOMI, GOME‐2 (total column), SCIAMACHY, and OMPS‐LP (vertical profiles), chemical ozone loss was evaluated and compared with the previous record Arctic winter 2010/11. The polar‐vortex‐averaged total column ozone loss in 2019/20 reached 88 DU (23%) and 106 DU (28%) based upon observations and model, respectively, by the end of March, which was similar to that derived for 2010/11. The maximum ozone loss (∼80%) observed by OMPS‐LP was near the 450 K potential temperature level (∼18 km altitude). Because of the larger polar vortex area in March 2020 compared to March 2011 (about 25% at 450 K), ozone mass loss was larger in Arctic winter 2019/20. It is shown that Arctic cap temperatures were at a record 40‐year low in winter, suggesting that Arctic cold winters are getting colder. Tropical UTLS temperatures show an apparent upward trend during the last two decades that may indicate a weakening of the Brewer‐Dobson circulation and strengthening of the polar vortex.
Transport‐Controlled Dissolution in an Evolving Fracture: The Extended Purday Solution and Fracture Flow Tests
Authors: Li Wei , Germaine John T , Einstein Herbert H.
Published: March 2021

Understanding the controlling mechanism and the resulting rate of reactive transport processes is crucial for an accurate prediction of the evolution of the rock‐fluid system in many geological processes and engineering applications. In this study, transport‐controlled dissolution in a single fracture was investigated analytically with the development of the extended Purday solution and experimentally with fracture flow tests. The extended Purday solution simulates dissolution in an evolving fracture and extends the validity domain of the Purday solution from a fracture with a uniform aperture to a fracture with aperture heterogeneity in the flow direction. The fracture flow tests include continuous effluent concentration measurements with a novel experimental setup and three‐dimensional fracture geometry analysis. The modeling and experimental results agree well and show that the high dissolution rate in the entrance region results in a converging fracture geometry (decreasing aperture in the flow direction). This converging geometry, in turn, reduces the overall dissolution rate in the fracture. The comparison between the modeling and experimental results shows that channel formation and sidewalls affect the morphology of the fracture. The resulting cross‐section geometry of the fracture also tends to reduce the overall dissolution rate. This study shows that the extended Purday solution accurately predicts the dissolution rate in an evolving fracture, and that factors, such as channel formation and sidewalls, affect fracture morphology and reduce the overall dissolution rate.
A novel class of sulfur-containing aminolipids widespread in marine roseobacters
Authors: Smith Alastair F , Silvano Eleonora , Päuker Orsola , Guillonneau Richard , Quareshy Mussa , Murphy Andrew , Mausz Michaela A , Stirrup Rachel , Rihtman Branko , Aguilo-Ferretjans Maria , Brandsma Joost , Petersen Jörn , Scanlan David J , Chen Yin
Published: March 2021

Marine roseobacter group bacteria are numerically abundant and ecologically important players in ocean ecosystems. These bacteria are capable of modifying their membrane lipid composition in response to environmental change. Remarkably, a variety of lipids are produced in these bacteria, including phosphorus-containing glycerophospholipids and several amino acid-containing aminolipids such as ornithine lipids and glutamine lipids. Here, we present the identification and characterization of a novel sulfur-containing aminolipid (SAL) in roseobacters. Using high resolution accurate mass spectrometry, a SAL was found in the lipid extract of Ruegeria pomeroyi DSS-3 and Phaeobacter inhibens DSM 17395. Using comparative genomics, transposon mutagenesis and targeted gene knockout, we identified a gene encoding a putative lyso-lipid acyltransferase, designated salA, which is essential for the biosynthesis of this SAL. Multiple sequence analysis and structural modeling suggest that SalA is a novel member of the lysophosphatidic acid acyltransferase (LPAAT) family, the prototype of which is the PlsC acyltransferase responsible for the biosynthesis of the phospholipid phosphatidic acid. SAL appears to play a key role in biofilm formation in roseobacters. salA is widely distributed in Tara Oceans metagenomes and actively expressed in Tara Oceans metatranscriptomes. Our results raise the importance of sulfur-containing membrane aminolipids in marine bacteria.
Infectivity, susceptibility, and risk factors associated with SARS-CoV-2 transmission under intensive contact tracing in Hunan, China
Authors: Hu Shixiong , Wang Wei , Wang Yan , Litvinova Maria , Luo Kaiwei , Ren Lingshuang , Sun Qianlai , Chen Xinghui , Zeng Ge , Li Jing , Liang Lu , Deng Zhihong , Zheng Wen , Li Mei , Yang Hao , Guo Jinxin , Wang Kai , Chen Xinhua , Liu Ziyan , Yan Han , Shi Huilin , Chen Zhiyuan , Zhou Yonghong , Sun Kaiyuan , Vespignani Alessandro , Viboud Cécile , Gao Lidong , Ajelli Marco , Yu Hongjie
Published: March 2021

Several mechanisms driving SARS-CoV-2 transmission remain unclear. Based on individual records of 1178 potential SARS-CoV-2 infectors and their 15,648 contacts in Hunan, China, we estimated key transmission parameters. The mean generation time was estimated to be 5.7 (median: 5.5, IQR: 4.5, 6.8) days, with infectiousness peaking 1.8 days before symptom onset, with 95% of transmission events occurring between 8.8 days before and 9.5 days after symptom onset. Most transmission events occurred during the pre-symptomatic phase (59.2%). SARS-CoV-2 susceptibility to infection increases with age, while transmissibility is not significantly different between age groups and between symptomatic and asymptomatic individuals. Contacts in households and exposure to first-generation cases are associated with higher odds of transmission. Our findings support the hypothesis that children can effectively transmit SARS-CoV-2 and highlight how pre-symptomatic and asymptomatic transmission can hinder control efforts.
Evaluating the effect of spaceflight on the host–pathogen interaction between human intestinal epithelial cells and Salmonella Typhimurium
Authors: Barrila Jennifer , Sarker Shameema F. , Hansmeier Nicole , Yang Shanshan , Buss Kristina , Briones Natalia , Park Jin , Davis Richard R. , Forsyth Rebecca J. , Ott C. Mark , Sato Kevin , Kosnik Cristine , Yang Anthony , Shimoda Cheryl , Rayl Nicole , Ly Diana , Landenberger Aaron , Wilson Stephanie D. , Yamazaki Naoko , Steel Jason , Montano Camila , Halden Rolf U. , Cannon Tom , Castro-Wallace Sarah L. , Nickerson Cheryl A.
Published: March 2021

Spaceflight uniquely alters the physiology of both human cells and microbial pathogens, stimulating cellular and molecular changes directly relevant to infectious disease. However, the influence of this environment on host–pathogen interactions remains poorly understood. Here we report our results from the STL-IMMUNE study flown aboard Space Shuttle mission STS-131, which investigated multi-omic responses (transcriptomic, proteomic) of human intestinal epithelial cells to infection withSalmonellaTyphimurium when both host and pathogen were simultaneously exposed to spaceflight. To our knowledge, this was the first in-flight infection and dual RNA-seq analysis using human cells.
Benchmarking surface tension measurement method using two oscillation modes in levitated liquid metals
Authors: Brosius Nevin , Ward Kevin , Wilson Evan , Karpinsky Zachary , SanSoucie Michael , Ishikawa Takehiko , Matsumoto Satoshi , Narayanan Ranga
Published: March 2021

The Faraday forcing method in levitated liquid droplets has recently been introduced as a method for measuring surface tension using resonance. By subjecting an electrostatically levitated liquid metal droplet to a continuous, oscillatory, electric field, at a frequency nearing that of the droplet’s first principal mode of oscillation (known as mode 2), the method was previously shown to determine surface tension of materials that would be particularly difficult to process by other means, e.g., liquid metals and alloys. It also offers distinct advantages in future work involving high viscosity samples because of the continuous forcing approach. This work presents (1) a benchmarking experimental method to measure surface tension by excitation of the second principal mode of oscillation (known as mode 3) in a levitated liquid droplet and (2) a more rigorous quantification of droplet excitation using a projection method. Surface tension measurements compare favorably to literature values for Zirconium, Inconel 625, and Rhodium, using both modes 2 and 3. Thus, this new method serves as a credible, self-consistent benchmarking technique for the measurement of surface tension.
Performance characteristics and costs of serological tests for brucellosis in a pastoralist community of northern Tanzania
Authors: Lukambagire AbdulHamid S. , Mendes Ângelo J. , Bodenham Rebecca F. , McGiven John A. , Mkenda Nestory A. , Mathew Coletha , Rubach Matthew P. , Sakasaka Philoteus , Shayo Davis D. , Maro Venance P. , Shirima Gabriel M. , Thomas Kate M. , Kasanga Christopher J. , Kazwala Rudovick R. , Halliday Jo E. B. , Mmbaga Blandina T.
Published: March 2021

The control of brucellosis across sub-Saharan Africa is hampered by the lack of standardized testing and the use of tests with poor performance. This study evaluated the performance and costs of serological assays for human brucellosis in a pastoralist community in northern Tanzania. Serum collected from 218 febrile hospital patients was used to evaluate the performance of seven index tests, selected based on international recommendation or current use. We evaluated the Rose Bengal test (RBT) using two protocols, four commercial agglutination tests and a competitive enzyme-linked immunosorbent assay (cELISA). The sensitivity, specificity, positive predictive value, negative predictive value, Youden’s index, diagnostic accuracy, and per-sample cost of each index test were estimated. The diagnostic accuracy estimates ranged from 95.9 to 97.7% for the RBT, 55.0 to 72.0% for the commercial plate tests, and 89.4% for the cELISA. The per-sample cost range was $0.69–$0.79 for the RBT, $1.03–$1.14 for the commercial plate tests, and $2.51 for the cELISA. The widely used commercial plate tests performed poorly and cost more than the RBT. These findings provide evidence for the public health value of discontinuing the use of commercial agglutination tests for human brucellosis in Tanzania.
Metallothionein-3 promotes cisplatin chemoresistance remodelling in neuroblastoma
Authors: Rodrigo Miguel Angel Merlos , Michalkova Hana , Strmiska Vladislav , Casar Berta , Crespo Piero , de los Rios Vivian , Ignacio Casal J. , Haddad Yazan , Guran Roman , Eckschlager Tomas , Pokorna Petra , Heger Zbynek , Adam Vojtech
Published: March 2021

Metallothionein-3 has poorly characterized functions in neuroblastoma. Cisplatin-based chemotherapy is a major regimen to treat neuroblastoma, but its clinical efficacy is limited by chemoresistance. We investigated the impact of human metallothionein-3 (hMT3) up-regulation in neuroblastoma cells and the mechanisms underlying the cisplatin-resistance. We confirmed the cisplatin-metallothionein complex formation using mass spectrometry. Overexpression of hMT3 decreased the sensitivity of neuroblastoma UKF-NB-4 cells to cisplatin. We report, for the first time, cisplatin-sensitive human UKF-NB-4 cells remodelled into cisplatin-resistant cells via high and constitutive hMT3 expression in an in vivo model using chick chorioallantoic membrane assay. Comparative proteomic analysis demonstrated that several biological pathways related to apoptosis, transport, proteasome, and cellular stress were involved in cisplatin-resistance in hMT3 overexpressing UKF-NB-4 cells. Overall, our data confirmed that up-regulation of hMT3 positively correlated with increased cisplatin-chemoresistance in neuroblastoma, and a high level of hMT3 could be one of the causes of frequent tumour relapses.
Eltrombopag for the treatment of refractory thrombocytopenia associated with connective tissue disease
Authors: Wang Juan , Dai Min , Fu Qiong , Chen Sheng
Published: March 2021

To assess the efficacy and safety of eltrombopag in connective tissue disease (CTD)-immune thrombocytopenia (ITP), we conducted this single-center retrospective observational study, including patients with refractory CTD-ITP who were treated with eltrombopag between January 2018 and August 2019. The characteristics of patients at baseline, and the efficacy and safety of the drug were analyzed. The predictors for a response were analyzed using a univariate analysis such as Chi-square or nonparametric test and a multiple correspondence analysis (MCA) method. A total of 15 patients with refractory CTD-ITP were included in the study. Their median age at the time of inclusion was 40.6 years. The median platelet count at initiation of eltrombopag was 11.53 × 109/L. The median remission time was 3.42 weeks. The complete remission (CR) and overall response rate decreased with time. The factors that associated with response to eltrombopag in patients with CTD-ITP were protopathy, WBC counts, levels of hemoglobin, and characteristics of bone marrow findings in univariate analysis. In addition, MCA indicated that a poor response to eltrombopag in patients with refractory CTD-ITP was closely associated with a protopathy with SS, medium to severe degree of anemia, leukopenia, and bone marrow aspiration showing aplastic anemia, an absence of megakaryocytes or macrophage activation syndrome (MAS). In conclusion, eltrombopag was effective and well-tolerated in patients with CTD-associated thrombocytopenia. Some factors should be considered in the use of eltrombopag, including the protopathy, blood test, and bone marrow histology.
Removing auto-activators from yeast-two-hybrid assays by conditional negative selection
Authors: Shivhare Devendra , Musialak-Lange Magdalena , Julca Irene , Gluza Pawel , Mutwil Marek
Published: March 2021

Yeast-two-hybrid (Y2H) is widely used as a strategy to detect protein–protein interactions (PPIs). Recent advancements have made it possible to generate and analyse genome-wide PPI networks en masse by coupling Y2H with next-generation sequencing technology. However, one of the major challenges of yeast two-hybrid assay is the large amount of false-positive hits caused by auto-activators (AAs), which are proteins that activate the reporter genes without the presence of an interacting protein partner. Here, we have developed a negative selection to minimize these auto-activators by integrating the pGAL2-URA3 fragment into the yeast genome. Upon activation of the pGAL2 promoter by an AA, yeast cells expressing URA3 cannot grow in media supplemented with 5-Fluoroorotic acid (5-FOA). Hence, we selectively inhibit the growth of yeast cells expressing auto-activators and thus minimizing the amount of false-positive hits. Here, we have demonstrated that auto-activators can be successfully removed from a Marchantia polymorpha cDNA library using pGAL2-URA3 and 5-FOA treatment, in liquid and solid-grown cultures. Furthermore, since URA3 can also serve as a marker for uracil autotrophy, we propose that our approach is a valuable addition to any large-scale Y2H screen.
Deep learning classification of lung cancer histology using CT images
Authors: Chaunzwa Tafadzwa L. , Hosny Ahmed , Xu Yiwen , Shafer Andrea , Diao Nancy , Lanuti Michael , Christiani David C. , Mak Raymond H. , Aerts Hugo J. W. L.
Published: March 2021

Tumor histology is an important predictor of therapeutic response and outcomes in lung cancer. Tissue sampling for pathologist review is the most reliable method for histology classification, however, recent advances in deep learning for medical image analysis allude to the utility of radiologic data in further describing disease characteristics and for risk stratification. In this study, we propose a radiomics approach to predicting non-small cell lung cancer (NSCLC) tumor histology from non-invasive standard-of-care computed tomography (CT) data. We trained and validated convolutional neural networks (CNNs) on a dataset comprising 311 early-stage NSCLC patients receiving surgical treatment at Massachusetts General Hospital (MGH), with a focus on the two most common histological types: adenocarcinoma (ADC) and Squamous Cell Carcinoma (SCC). The CNNs were able to predict tumor histology with an AUC of 0.71(p = 0.018). We also found that using machine learning classifiers such as k-nearest neighbors (kNN) and support vector machine (SVM) on CNN-derived quantitative radiomics features yielded comparable discriminative performance, with AUC of up to 0.71 (p = 0.017). Our best performing CNN functioned as a robust probabilistic classifier in heterogeneous test sets, with qualitatively interpretable visual explanations to its predictions. Deep learning based radiomics can identify histological phenotypes in lung cancer. It has the potential to augment existing approaches and serve as a corrective aid for diagnosticians.
A multicenter, prospective, observational study to determine association of mesangial C1q deposition with renal outcomes in IgA nephropathy
Authors: Tan Li , Tang Yi , Pei Gaiqin , Zhong Zhengxia , Tan Jiaxing , Zhou Ling , Wen Dongmei , Sheikh-Hamad David , Qin Wei
Published: March 2021

It was reported that histopathologic lesions are risk factors for the progression of IgA Nephropathy (IgAN). The aim of this study was to investigate the relationships between mesangial deposition of C1q and renal outcomes in IgAN. 1071 patients with primary IgAN diagnosed by renal biopsy were enrolled in multiple study centers form January 2013 to January 2017. Patients were divided into two groups: C1q-positive and C1q-negative. Using a 1: 4 propensity score matching (PSM) method identifying age, gender, and treatment modality to minimize confounding factors, 580 matched (out of 926) C1q-negative patients were compared with 145 C1q-positive patients to evaluate severity of baseline clinicopathological features and renal outcome. Kaplan–Meier and Cox proportional hazards analyses were performed to determine whether mesangial C1q deposition is associated with renal outcomes in IgAN. During the follow-up period (41.89 ± 22.85 months), 54 (9.31%) patients in the C1q negative group and 23 (15.86%) patients in C1q positive group reached the endpoint (50% decline of eGFR and/or ESRD or death) respectively (p = 0.01) in the matched cohort. Significantly more patients in C1q negative group achieved complete or partial remission during the follow up period (P = 0.003) both before and after PSM. Three, 5 and 7-year renal survival rates in C1q-positive patients were significantly lower than C1q-negative patients in either unmatched cohort or matched cohort (all p < 0.05). Furthermore, multivariate Cox regression analysis showed that independent risk factors influencing renal survival included Scr, urinary protein, T1-T2 lesion and C1q deposition. Mesangial C1q deposition is a predictor of poor renal survival in IgA nephropathy.

Trial registration TCTR, TCTR20140515001. Registered May 15, 2014, http://www.clinicaltrials.in.th/index.php?tp=regtrials&menu=trialsearch&smenu=fulltext&task=search&task2=view1&id=1074.
Swine growth promotion with antibiotics or alternatives can increase antibiotic resistance gene mobility potential
Authors: Muurinen Johanna , Richert Jacob , Wickware Carmen L. , Richert Brian , Johnson Timothy A.
Published: March 2021

Even though the use of antibiotics for food-producing animals may contribute to the emergence of antimicrobial resistance, antibiotics are still used as growth promoters. Due to consumer and regulatory pressures, the use of alternatives to antibiotics as growth promoters is increasing, thus more information is needed on their capability to disseminate antimicrobial resistance compared to antibiotics. We investigated the impacts of carbadox (antibiotic), copper sulfate and zinc oxide (metals) and mushroom powder (natural product) on the pig fecal resistome and microbiome. Antibiotic resistance gene (ARG) and mobile genetic element (MGE) abundances were measured using a high-throughput qPCR array with 382 primer pairs. Bacterial community composition was determined by 16S rRNA gene sequencing. More ARGs co-occurred with MGEs in the growth promoter group samples than in the control group samples. Community composition could not be linked to resistome in the growth promoter group samples, indicating a potential decoupling of ARGs and phylogeny. Additionally, machine-learning methods aided in defining the community and resistome differences in response to treatments. Since increased ARG mobility potential was the primary response to the dietary additives used in this study, we suggest that ARG mobility should be considered when designing antimicrobial use policies and antimicrobial resistance surveillances.
Development of sensitive and accurate solid-phase microextraction procedure for preconcentration of As(III) ions in real samples
Authors: Elik Adil , Tuzen Mustafa , Hazer Baki , Kaya Savaş , Katin K. P. , Altunay Nail
Published: March 2021

We synthesized the poly(methyl methacrylate-co-2-aminoethyl methacrylate (PMaema) amphiphilic copolymer in a form of solid phase adsorbent. Then it was used for separation, preconcentration and determination of trace amount of As(III) ions from foods and waters with hydride generation atomic absorption spectrometry. The PMaema was characterized by fourier transform infrared spectrometer and nuclear magnetic resonance spectrometer. The adsorption of As(III) to the PMaema was also supported using computational chemistry studies. The experimental parameters (pH, PMaema amount, adsorption time and ethanol volume) were optimized using a three-level Box–Behnken design with four experimental factors. We observed linear calibration curve for the PMaema amount in the 10–500 ng L−1 range (R2 = 0.9956). Limit of detection, preconcentration factor and sorbent capacity of PMaema were equal to 3.3 ng L−1, 100 and 75.8 mg g−1, respectively. The average recoveries (spiked at 50 ng L−1) changes in the range of 91.5–98.6% with acceptable relative standard deviation less than 4.3%. After validation studies, the method was successfully applied for separation, preconcentration and determination of trace amount of As(III) from foods and waters.
East Antarctica magnetically linked to its ancient neighbours in Gondwana
Authors: Ebbing Jörg , Dilixiati Yixiati , Haas Peter , Ferraccioli Fausto , Scheiber-Enslin Stephanie
Published: March 2021

We present a new magnetic compilation for Central Gondwana conformed to a recent satellite magnetic model (LCS-1) with the help of an equivalent layer approach, resulting in consistent levels, corrections that have not previously been applied. Additionally, we use the satellite data to its full spectral content, which helps to include India, where high resolution aeromagnetic data are not publically available. As India is located north of the magnetic equator, we also performed a variable reduction to the pole to the satellite data by applying an equivalent source method. The conformed aeromagnetic and satellite data are superimposed on a recent deformable Gondwana plate reconstruction that links the Kaapvaal Craton in Southern Africa with the Grunehogna Craton in East Antarctica in a tight fit. Aeromagnetic anomalies unveil, however, wider orogenic belts that preserve remnants of accreted Meso- to Neoproterozoic crust in interior East Antarctica, compared to adjacent sectors of Southern Africa and India. Satellite and aeromagnetic anomaly datasets help to portray the extent and architecture of older Precambrian cratons, re-enforcing their linkages in East Antarctica, Australia, India and Africa.
Corneal proteome and differentially expressed corneal proteins in highly myopic chicks using a label-free SWATH-MS quantification approach
Authors: Kang Byung Soo , Lam Thomas Chuen , Cheung Jimmy Ka-wai , Li King Kit , Kee Chea-su
Published: March 2021

Myopia, or short-sightedness, is a highly prevalent refractive disorder in which the eye’s focal length is too short for its axial dimension in its relaxed state. High myopia is associated with increased risks of blinding ocular complications and abnormal eye shape. In addition to consistent findings on posterior segment anomalies in high myopia (e.g., scleral remodeling), more recent biometric and biomechanical data in myopic humans and animal models also indicate anterior segment anomalies (e.g., corneal biomechanical properties). Because the cornea is the anterior-most ocular tissue, providing essential refractive power and physiological stability, it is important to understand the biochemical signaling pathway during myopia development. This study first aimed to establish the entire chicken corneal proteome. Then, using the classical form deprivation paradigm to induce high myopia in chicks, state-of-the-art bioinformatics technologies were applied to identify eight differentially expressed proteins in the highly myopic cornea. These results provide strong foundation for future corneal research, especially those using chicken as an animal model for myopia development.
Gut microbiota markers associated with obesity and overweight in Italian adults
Authors: Palmas Vanessa , Pisanu Silvia , Madau Veronica , Casula Emanuela , Deledda Andrea , Cusano Roberto , Uva Paolo , Vascellari Sarah , Loviselli Andrea , Manzin Aldo , Velluzzi Fernanda
Published: March 2021

In the present study, we characterized the distinctive signatures of the gut microbiota (GM) from overweight/obese patients (OB), and normal-weight controls (NW), both of Sardinian origin. Fecal bacterial composition of 46 OB patients (BMI = 36.6 ± 6.0; F/M = 40/6) was analyzed and compared to that of 46 NW subjects (BMI = 21.6 ± 2.1; F/M = 41/5), matched for sex, age and smoking status, by using 16S rRNA gene sequencing on MiSeq Illumina platform. The gut microbial community of OB patients exhibited a significant decrease in the relative abundance of several Bacteroidetes taxa (i.e. Flavobacteriaceae, Porphyromonadaceae, Sphingobacteriaceae, Flavobacterium, Rikenella spp., Pedobacter spp., Parabacteroides spp., Bacteroides spp.) when compared to NW; instead, several Firmicutes taxa were significantly increased in the same subjects (Lachnospiraceae, Gemellaceae, Paenibacillaceae, Streptococcaceae, Thermicanaceae, Gemella, Mitsuokella, Streptococcus, Acidaminococcus spp., Eubacterium spp., Ruminococcus spp., Megamonas spp., Streptococcus, Thermicanus, Megasphaera spp. and Veillonella spp.). Correlation analysis indicated that body fatness and waist circumference negatively correlated with Bacteroidetes taxa, while Firmicutes taxa positively correlated with body fat and negatively with muscle mass and/or physical activity level. Furthermore, the relative abundance of several bacterial taxa belonging to Enterobacteriaceae family, known to exhibit endotoxic activity, was increased in the OB group compared to NW. The results extend our knowledge on the GM profiles in Italian OB, identifying novel taxa linking obesity and intestine.