bluuebunny/crossref_metadata_2025 huggingface dataset:
Figure S9 from Treatment of Prostate Cancer with CD46-targeted 225Ac Alpha Particle Radioimmunotherapy
Authors: Bidkar Anil P. , Wang Sinan , Bobba Kondapa Naidu , Chan Emily , Bidlingmaier Scott , Egusa Emily A. , Peter Robin , Ali Umama , Meher Niranjan , Wadhwa Anju , Dhrona Suchi , Dasari Chandrashekhar , Beckford-Vera Denis , Su Yang , Tang Ryan , Zhang Li , He Jiang , Wilson David M. , Aggarwal Rahul , VanBrocklin Henry F. , Seo Youngho , Chou Jonathan , Liu Bin , Flavell Robert R.
Published: April 2023

Figure S9: Histology evaluation of the healthy tissues for long-term (117 days) toxicity analysis. Hematoxylin and Eosin (H&E) staining of spleen, liver, lungs, heart, and bone samples show no toxicity at 0.25 µCi or 0.5 µCi doses of [225Ac]DOTA-YS5. Scale bar: 20 µm
Figure S9 from A Phase II Trial of Guadecitabine plus Atezolizumab in Metastatic Urothelial Carcinoma Progressing after Initial Immune Checkpoint Inhibitor Therapy
Authors: Jang H. Josh , Hostetter Galen , Macfarlane Alexander W. , Madaj Zachary , Ross Eric A. , Hinoue Toshinori , Kulchycki Justin R. , Burgos Ryan S. , Tafseer Mahvish , Alpaugh R. Katherine , Schwebel Candice L. , Kokate Rutika , Geynisman Daniel M. , Zibelman Matthew R. , Ghatalia Pooja , Nichols Peter W. , Chung Woonbok , Madzo Jozef , Hahn Noah M. , Quinn David I. , Issa Jean-Pierre J. , Topper Michael J. , Baylin Stephen B. , Shen Hui , Campbell Kerry S. , Jones Peter A. , Plimack Elizabeth R.
Published: April 2023

Supplementary Figure 9: CD8 infiltration in tumor is associated with survival.
Figure S6 from A Phase II Trial of Guadecitabine plus Atezolizumab in Metastatic Urothelial Carcinoma Progressing after Initial Immune Checkpoint Inhibitor Therapy
Authors: Jang H. Josh , Hostetter Galen , Macfarlane Alexander W. , Madaj Zachary , Ross Eric A. , Hinoue Toshinori , Kulchycki Justin R. , Burgos Ryan S. , Tafseer Mahvish , Alpaugh R. Katherine , Schwebel Candice L. , Kokate Rutika , Geynisman Daniel M. , Zibelman Matthew R. , Ghatalia Pooja , Nichols Peter W. , Chung Woonbok , Madzo Jozef , Hahn Noah M. , Quinn David I. , Issa Jean-Pierre J. , Topper Michael J. , Baylin Stephen B. , Shen Hui , Campbell Kerry S. , Jones Peter A. , Plimack Elizabeth R.
Published: April 2023

Supplementary Figure 6: Gene Set Enrichment Analysis reveals immune-related pathways discriminate PD and SD tumors.
Figure S5 from Treatment of Prostate Cancer with CD46-targeted 225Ac Alpha Particle Radioimmunotherapy
Authors: Bidkar Anil P. , Wang Sinan , Bobba Kondapa Naidu , Chan Emily , Bidlingmaier Scott , Egusa Emily A. , Peter Robin , Ali Umama , Meher Niranjan , Wadhwa Anju , Dhrona Suchi , Dasari Chandrashekhar , Beckford-Vera Denis , Su Yang , Tang Ryan , Zhang Li , He Jiang , Wilson David M. , Aggarwal Rahul , VanBrocklin Henry F. , Seo Youngho , Chou Jonathan , Liu Bin , Flavell Robert R.
Published: April 2023

Figure S5: Estimated equivalent doses (in Sv) in organs and tumor indicating the highest dose (37 Sv) was delivered to tumor tissue.
Figure S2 from A Phase II Trial of Guadecitabine plus Atezolizumab in Metastatic Urothelial Carcinoma Progressing after Initial Immune Checkpoint Inhibitor Therapy
Authors: Jang H. Josh , Hostetter Galen , Macfarlane Alexander W. , Madaj Zachary , Ross Eric A. , Hinoue Toshinori , Kulchycki Justin R. , Burgos Ryan S. , Tafseer Mahvish , Alpaugh R. Katherine , Schwebel Candice L. , Kokate Rutika , Geynisman Daniel M. , Zibelman Matthew R. , Ghatalia Pooja , Nichols Peter W. , Chung Woonbok , Madzo Jozef , Hahn Noah M. , Quinn David I. , Issa Jean-Pierre J. , Topper Michael J. , Baylin Stephen B. , Shen Hui , Campbell Kerry S. , Jones Peter A. , Plimack Elizabeth R.
Published: April 2023

Supplementary Figure 2: Modest treatment-induced DNA methylation changes in UC tumors.
Figure S11 from Treatment of Prostate Cancer with CD46-targeted 225Ac Alpha Particle Radioimmunotherapy
Authors: Bidkar Anil P. , Wang Sinan , Bobba Kondapa Naidu , Chan Emily , Bidlingmaier Scott , Egusa Emily A. , Peter Robin , Ali Umama , Meher Niranjan , Wadhwa Anju , Dhrona Suchi , Dasari Chandrashekhar , Beckford-Vera Denis , Su Yang , Tang Ryan , Zhang Li , He Jiang , Wilson David M. , Aggarwal Rahul , VanBrocklin Henry F. , Seo Youngho , Chou Jonathan , Liu Bin , Flavell Robert R.
Published: April 2023

Figure S11: Antitumor activity of [225Ac]DOTA-YS5 in subcutaneous DU145 tumors in nude mice. Tumor volume, survival analysis, and weight measurements after the injections of [225Ac]DOTA-YS5 in DU145 xenografts (n=6).
Figure S11 from A Phase II Trial of Guadecitabine plus Atezolizumab in Metastatic Urothelial Carcinoma Progressing after Initial Immune Checkpoint Inhibitor Therapy
Authors: Jang H. Josh , Hostetter Galen , Macfarlane Alexander W. , Madaj Zachary , Ross Eric A. , Hinoue Toshinori , Kulchycki Justin R. , Burgos Ryan S. , Tafseer Mahvish , Alpaugh R. Katherine , Schwebel Candice L. , Kokate Rutika , Geynisman Daniel M. , Zibelman Matthew R. , Ghatalia Pooja , Nichols Peter W. , Chung Woonbok , Madzo Jozef , Hahn Noah M. , Quinn David I. , Issa Jean-Pierre J. , Topper Michael J. , Baylin Stephen B. , Shen Hui , Campbell Kerry S. , Jones Peter A. , Plimack Elizabeth R.
Published: April 2023

Supplementary Figure 11: Peripheral immune cells are activated mid-treatment cycle.
Figure S10 from A Phase II Trial of Guadecitabine plus Atezolizumab in Metastatic Urothelial Carcinoma Progressing after Initial Immune Checkpoint Inhibitor Therapy
Authors: Jang H. Josh , Hostetter Galen , Macfarlane Alexander W. , Madaj Zachary , Ross Eric A. , Hinoue Toshinori , Kulchycki Justin R. , Burgos Ryan S. , Tafseer Mahvish , Alpaugh R. Katherine , Schwebel Candice L. , Kokate Rutika , Geynisman Daniel M. , Zibelman Matthew R. , Ghatalia Pooja , Nichols Peter W. , Chung Woonbok , Madzo Jozef , Hahn Noah M. , Quinn David I. , Issa Jean-Pierre J. , Topper Michael J. , Baylin Stephen B. , Shen Hui , Campbell Kerry S. , Jones Peter A. , Plimack Elizabeth R.
Published: April 2023

Supplementary Figure 10: Peripheral immune cell dynamics induced by combination therapy.
Figure S1 from Treatment of Prostate Cancer with CD46-targeted 225Ac Alpha Particle Radioimmunotherapy
Authors: Bidkar Anil P. , Wang Sinan , Bobba Kondapa Naidu , Chan Emily , Bidlingmaier Scott , Egusa Emily A. , Peter Robin , Ali Umama , Meher Niranjan , Wadhwa Anju , Dhrona Suchi , Dasari Chandrashekhar , Beckford-Vera Denis , Su Yang , Tang Ryan , Zhang Li , He Jiang , Wilson David M. , Aggarwal Rahul , VanBrocklin Henry F. , Seo Youngho , Chou Jonathan , Liu Bin , Flavell Robert R.
Published: April 2023

Figure S1: Immunohistochemical staining for PSMA expression on tissue section from patient-derived xenografts. Scale bar = 200 µm
Figure S8 from Preclinical Efficacy of the Antibody–Drug Conjugate CLDN6–23-ADC for the Treatment of CLDN6-Positive Solid Tumors
Authors: McDermott Martina S.J. , O'Brien Neil A. , Hoffstrom Benjamin , Gong KeWei , Lu Ming , Zhang Jun , Luo Tong , Liang Min , Jia Weiping , Hong Jenny J. , Chau Kevin , Davenport Simon , Xie Bin , Press Michael F. , Panayiotou Richard , Handly-Santana Abram , Brugge Joan S. , Presta Leonard , Glaspy John , Slamon Dennis J.
Published: April 2023

Supplemental Figure S8. A. Layout of tissues and B. Scanned whole-slide image of BN1021, normal human tissue microarray stained for CLDN6 expression showing no staining.
Figure S7 from Preclinical Efficacy of the Antibody–Drug Conjugate CLDN6–23-ADC for the Treatment of CLDN6-Positive Solid Tumors
Authors: McDermott Martina S.J. , O'Brien Neil A. , Hoffstrom Benjamin , Gong KeWei , Lu Ming , Zhang Jun , Luo Tong , Liang Min , Jia Weiping , Hong Jenny J. , Chau Kevin , Davenport Simon , Xie Bin , Press Michael F. , Panayiotou Richard , Handly-Santana Abram , Brugge Joan S. , Presta Leonard , Glaspy John , Slamon Dennis J.
Published: April 2023

Supplemental Figure S7. CLDN6 expression in ovarian and endometrial cancer tissue samples.
Figure S5 from Preclinical Efficacy of the Antibody–Drug Conjugate CLDN6–23-ADC for the Treatment of CLDN6-Positive Solid Tumors
Authors: McDermott Martina S.J. , O'Brien Neil A. , Hoffstrom Benjamin , Gong KeWei , Lu Ming , Zhang Jun , Luo Tong , Liang Min , Jia Weiping , Hong Jenny J. , Chau Kevin , Davenport Simon , Xie Bin , Press Michael F. , Panayiotou Richard , Handly-Santana Abram , Brugge Joan S. , Presta Leonard , Glaspy John , Slamon Dennis J.
Published: April 2023

Supplemental Figure S5: Induction of apoptosis following 48 hr treatment of CLDN6 positive OVCA429 and CLDN6 negative M202 cells with a range of concentrations (50 μg/ml - 0.3658μg/ml) of CLDN6-23-ADC compared to control IgG ADC.
Figure S3 from Preclinical Efficacy of the Antibody–Drug Conjugate CLDN6–23-ADC for the Treatment of CLDN6-Positive Solid Tumors
Authors: McDermott Martina S.J. , O'Brien Neil A. , Hoffstrom Benjamin , Gong KeWei , Lu Ming , Zhang Jun , Luo Tong , Liang Min , Jia Weiping , Hong Jenny J. , Chau Kevin , Davenport Simon , Xie Bin , Press Michael F. , Panayiotou Richard , Handly-Santana Abram , Brugge Joan S. , Presta Leonard , Glaspy John , Slamon Dennis J.
Published: April 2023

Supplemental Figure S3. A, Efficacy of anti-CLDN6 mouse antibodies in CLDN6 positive OV90 ovarian cancer cell line xenografts. B, Efficacy in CLDN6 positive UMUC4 bladder cancer cell line xenografts. C, No efficacy in CLDN6 negative M202 melanoma cell line xenografts. All antibodies are dosed at 10 mg/kg QW IV in each study. Errors bars represent SEM of 8 replicate animals per group.
Supplementary Table S4 from Increased Nerve Density Adversely Affects Outcome in Oral Cancer
Authors: Perez-Pacheco Cindy , Schmitd Ligia B. , Furgal Allison , Bellile Emily L. , Liu Min , Fattah Aya , Gonzalez-Maldonado Laura , Unsworth Shelby P. , Wong Sunny Y. , Rozek Laura S. , Rao Arvind , Wolf Gregory T. , Taylor Jeremy M.G. , Casper Keith , Mierzwa Michelle , D'Silva Nisha J.
Published: April 2023

Adjusted Cox regression modeling of normalized nerve density using AJCC 8th edition
Supplementary Table S2 from Increased Nerve Density Adversely Affects Outcome in Oral Cancer
Authors: Perez-Pacheco Cindy , Schmitd Ligia B. , Furgal Allison , Bellile Emily L. , Liu Min , Fattah Aya , Gonzalez-Maldonado Laura , Unsworth Shelby P. , Wong Sunny Y. , Rozek Laura S. , Rao Arvind , Wolf Gregory T. , Taylor Jeremy M.G. , Casper Keith , Mierzwa Michelle , D'Silva Nisha J.
Published: April 2023

Adjusted Cox modeling of intratumoral and normalized nerve density in tongue SCC using AJCC 8th edition
Supplementary Table S1 from Increased Nerve Density Adversely Affects Outcome in Oral Cancer
Authors: Perez-Pacheco Cindy , Schmitd Ligia B. , Furgal Allison , Bellile Emily L. , Liu Min , Fattah Aya , Gonzalez-Maldonado Laura , Unsworth Shelby P. , Wong Sunny Y. , Rozek Laura S. , Rao Arvind , Wolf Gregory T. , Taylor Jeremy M.G. , Casper Keith , Mierzwa Michelle , D'Silva Nisha J.
Published: April 2023

Adjusted Cox modeling of intratumoral and normalized nerve density in tongue SCC using AJCC 7th edition
Data from A Phase II Trial of Guadecitabine plus Atezolizumab in Metastatic Urothelial Carcinoma Progressing after Initial Immune Checkpoint Inhibitor Therapy
Authors: Jang H. Josh , Hostetter Galen , Macfarlane Alexander W. , Madaj Zachary , Ross Eric A. , Hinoue Toshinori , Kulchycki Justin R. , Burgos Ryan S. , Tafseer Mahvish , Alpaugh R. Katherine , Schwebel Candice L. , Kokate Rutika , Geynisman Daniel M. , Zibelman Matthew R. , Ghatalia Pooja , Nichols Peter W. , Chung Woonbok , Madzo Jozef , Hahn Noah M. , Quinn David I. , Issa Jean-Pierre J. , Topper Michael J. , Baylin Stephen B. , Shen Hui , Campbell Kerry S. , Jones Peter A. , Plimack Elizabeth R.
Published: April 2023

AbstractPurpose:
On the basis of preclinical evidence of epigenetic contribution to sensitivity and resistance to immune checkpoint inhibitors (ICI), we hypothesized that guadecitabine (hypomethylating agent) and atezolizumab [anti–programmed cell death ligand 1 (PD-L1)] together would potentiate a clinical response in patients with metastatic urothelial carcinoma (UC) unresponsive to initial immune checkpoint blockade therapy.
Patients and Methods:
We designed a single arm phase II study (NCT03179943) with a safety run-in to identify the recommended phase II dose of the combination therapy of guadecitabine and atezolizumab. Patients with recurrent/advanced UC who had previously progressed on ICI therapy with programmed cell death protein 1 or PD-L1 targeting agents were eligible. Preplanned correlative analysis was performed to characterize peripheral immune dynamics and global DNA methylation, transcriptome, and immune infiltration dynamics of patient tumors.
Results:
Safety run-in enrolled 6 patients and phase II enrolled 15 patients before the trial was closed for futility. No dose-limiting toxicity was observed. Four patients, with best response of stable disease (SD), exhibited extended tumor control (8–11 months) and survival (>14 months). Correlative analysis revealed lack of DNA demethylation in tumors after 2 cycles of treatment. Increased peripheral immune activation and immune infiltration in tumors after treatment correlated with progression-free survival and SD. Furthermore, high IL6 and IL8 levels in the patients’ plasma was associated with short survival.
Conclusions:
No RECIST responses were observed after combination therapy in this trial. Although we could not detect the anticipated tumor-intrinsic effects of guadecitabine, the addition of hypomethylating agent to ICI therapy induced immune activation in a few patients, which associated with longer patient survival.
Data from Preclinical Efficacy of the Antibody–Drug Conjugate CLDN6–23-ADC for the Treatment of CLDN6-Positive Solid Tumors
Authors: McDermott Martina S.J. , O'Brien Neil A. , Hoffstrom Benjamin , Gong KeWei , Lu Ming , Zhang Jun , Luo Tong , Liang Min , Jia Weiping , Hong Jenny J. , Chau Kevin , Davenport Simon , Xie Bin , Press Michael F. , Panayiotou Richard , Handly-Santana Abram , Brugge Joan S. , Presta Leonard , Glaspy John , Slamon Dennis J.
Published: April 2023

AbstractPurpose:
Claudin-6 (CLDN6) is expressed at elevated levels in multiple human cancers including ovarian and endometrial malignancies, with little or no detectable expression in normal adult tissue. This expression profile makes CLDN6 an ideal target for development of a potential therapeutic antibody–drug conjugate (ADC). This study describes the generation and preclinical characterization of CLDN6–23-ADC, an ADC consisting of a humanized anti-CLDN6 monoclonal antibody coupled to monomethyl auristatin E (MMAE) via a cleavable linker.
Experimental Design:
A fully humanized anti-CLDN6 antibody was conjugated to MMAE resulting in the potential therapeutic ADC, CLDN6–23-ADC. The antitumor efficacy of CLDN6–23-ADC was assessed for antitumor efficacy in CLDN6-positive (CLDN6+) and -negative (CLDN6−) xenografts and patient-derived xenograft (PDX) models of human cancers.
Results:
CLDN6–23-ADC selectively binds to CLDN6, versus other CLDN family members, inhibits the proliferation of CLDN6+ cancer cells in vitro, and is rapidly internalized in CLDN6+ cells. Robust tumor regressions were observed in multiple CLDN6+ xenograft models and tumor inhibition led to markedly enhanced survival of CLDN6+ PDX tumors following treatment with CLDN6–23-ADC. IHC assessment of cancer tissue microarrays demonstrate elevated levels of CLDN6 in 29% of ovarian epithelial carcinomas. Approximately 45% of high-grade serous ovarian carcinomas and 11% of endometrial carcinomas are positive for the target.
Conclusions:
We report the development of a novel ADC, CLDN6–23-ADC, that selectively targets CLDN6, a potential onco-fetal-antigen which is highly expressed in ovarian and endometrial cancers. CLDN6–23-ADC exhibits robust tumor regressions in mouse models of human ovarian and endometrial cancers and is currently undergoing phase I study.
Data from Increased Nerve Density Adversely Affects Outcome in Oral Cancer
Authors: Perez-Pacheco Cindy , Schmitd Ligia B. , Furgal Allison , Bellile Emily L. , Liu Min , Fattah Aya , Gonzalez-Maldonado Laura , Unsworth Shelby P. , Wong Sunny Y. , Rozek Laura S. , Rao Arvind , Wolf Gregory T. , Taylor Jeremy M.G. , Casper Keith , Mierzwa Michelle , D'Silva Nisha J.
Published: April 2023

AbstractPurpose:
Perineural invasion (PNI) in oral cavity squamous cell carcinoma (OSCC) is associated with poor survival. Because of the risk of recurrence, patients with PNI receive additional therapies after surgical resection. Mechanistic studies have shown that nerves in the tumor microenvironment promote aggressive tumor growth. Therefore, in this study, we evaluated whether nerve density (ND) influences tumor growth and patient survival. Moreover, we assessed the reliability of artificial intelligence (AI) in evaluating ND.
Experimental Design:
To investigate whether increased ND in OSCC influences patient outcome, we performed survival analyses. Tissue sections of OSCC from 142 patients were stained with hematoxylin and eosin and IHC stains to detect nerves and tumor. ND within the tumor bulk and in the adjacent 2 mm was quantified; normalized ND (NND; bulk ND/adjacent ND) was calculated. The impact of ND on tumor growth was evaluated in chick chorioallantoic-dorsal root ganglia (CAM-DRG) and murine surgical denervation models. Cancer cells were grafted and tumor size quantified. Automated nerve detection, applying the Halo AI platform, was compared with manual assessment.
Results:
Disease-specific survival decreased with higher intratumoral ND and NND in tongue SCC. Moreover, NND was associated with worst pattern-of-invasion and PNI. Increasing the number of DRG, in the CAM-DRG model, increased tumor size. Reduction of ND by denervation in a murine model decreased tumor growth. Automated and manual detection of nerves showed high concordance, with an F1 score of 0.977.
Conclusions:
High ND enhances tumor growth, and NND is an important prognostic factor that could influence treatment selection for aggressive OSCC.

See related commentary by Hondermarck and Jiang, p. 2342
Antitumor Efficacy of Dual Blockade with Encorafenib + Cetuximab in Combination with Chemotherapy in Human BRAFV600E-Mutant Colorectal Cancer
Authors: Napolitano Stefania , Woods Melanie , Lee Hey Min , De Falco Vincenzo , Martini Giulia , Della Corte Carminia Maria , Martinelli Erika , Famiglietti Vincenzo , Ciardiello Davide , Anderson Amanda , Fowlkes Natalie Wall , Villareal Oscar Eduardo , Sorokin Alexey , Kanikarla Preeti , Coker Olu , Morris Van , Altucci Lucia , Tabernero Josep , Troiani Teresa , Ciardiello Fortunato , Kopetz Scott
Published: April 2023

Purpose:

Encorafenib + cetuximab (E+C) is an effective therapeutic option in chemorefractory BRAFV600E metastatic colorectal cancer (mCRC). However, there is a need to improve the efficacy of this molecular-targeted therapy and evaluate regimens suitable for untreated BRAFV600E in patients with mCRC.

Experimental Design:

We performed a series of in vivo studies using BRAFV600E mCRC tumor xenografts. Mice were randomized to receive 5-fluoruracil (5-FU), irinotecan, or oxaliplatin regimens (FOLFIRI or FOLFOX), (E+C) or the combination. Patients received long-term treatment until disease progression, with deescalation strategies used to mimic maintenance therapy. Transcriptomic changes after progression on cytotoxic chemotherapy or targeted therapy were assessed.

Results:

Antitumor activity of either FOLFIRI or E+C was better as first-line treatment as compared with second-line, with partial cross-resistance seen between a cytotoxic regimen and targeted therapy with an average 62% loss of efficacy for FOLFIRI after E+C and a 45% loss of efficacy of E+C after FOLFIRI (P < 0.001 for both). FOLFIRI-treated models had upregulation of epithelial–mesenchymal transition (EMT) and MAPK pathway activation, where E+C treated models had suppressed MAPK signaling. In contrast, with chemotherapy with E+C, EMT and MAPK signaling remained suppressed. FOLFOX or FOLFIRI, each in combination with E+C, were the most active first-line treatments as compared with E+C or to chemotherapy alone. Furthermore, FOLFOX in combination with E+C as first-line induction therapy, followed by E+C ± 5-FU as maintenance therapy, was the most effective strategy for long-term disease control.

Conclusions:

These results support the combination of cytotoxic chemotherapy and molecular-targeted therapy as a promising therapeutic approach in the first-line treatment of BRAFV600E mCRC.
Cancer's Dark Matter: Lighting the Abyss Unveils Universe of New Therapies
Authors: Fox Bernard A. , Urba Walter J. , Jensen Shawn M. , Page David B. , Curti Brendan D. , Sanborn Rachel E. , Leidner Rom S.
Published: April 2023

The authors of a recent study identified noncanonical peptides (NCP) presented by cancer cells’ HLA and observed lack of reactivity to these antigens by endogenous tumor-reactive T cells. In vitro sensitization generated NCP-reactive T cells that recognized epitopes shared by a majority of cancers tested, providing opportunities for novel therapies to shared antigens.

See related article by Lozano-Rabella et al., p. 2250
Supplementary Table S9 from In Vivo Screening Unveils Pervasive RNA-Binding Protein Dependencies in Leukemic Stem Cells and Identifies ELAVL1 as a Therapeutic Target
Authors: Vujovic Ana , de Rooij Laura , Chahi Ava Keyvani , Chen He Tian , Yee Brian A. , Loganathan Sampath K. , Liu Lina , Chan Derek C.H. , Tajik Amanda , Tsao Emily , Moreira Steven , Joshi Pratik , Xu Joshua , Wong Nicholas , Balde Zaldy , Jahangiri Soheil , Zandi Sasan , Aigner Stefan , Dick John E. , Minden Mark D. , Schramek Daniel , Yeo Gene W. , Hope Kristin J.
Published: April 2023

Table S9 shows the (a) differentially expressed transcripts and (b) altered pathways as identified by GSEA from the ELAVL1 knockdown human primary AML RNA-sequencing data set.
Supplementary Table S8 from In Vivo Screening Unveils Pervasive RNA-Binding Protein Dependencies in Leukemic Stem Cells and Identifies ELAVL1 as a Therapeutic Target
Authors: Vujovic Ana , de Rooij Laura , Chahi Ava Keyvani , Chen He Tian , Yee Brian A. , Loganathan Sampath K. , Liu Lina , Chan Derek C.H. , Tajik Amanda , Tsao Emily , Moreira Steven , Joshi Pratik , Xu Joshua , Wong Nicholas , Balde Zaldy , Jahangiri Soheil , Zandi Sasan , Aigner Stefan , Dick John E. , Minden Mark D. , Schramek Daniel , Yeo Gene W. , Hope Kristin J.
Published: April 2023

Table S8 shows transcripts from the ELAVL1 knockout RNA-sequencing data set that are (a) alternatively spliced and (b) alternatively spliced as well as bound by ELAVL1 as identified by eCLIP-sequencing.
Supplementary Table S6 from In Vivo Screening Unveils Pervasive RNA-Binding Protein Dependencies in Leukemic Stem Cells and Identifies ELAVL1 as a Therapeutic Target
Authors: Vujovic Ana , de Rooij Laura , Chahi Ava Keyvani , Chen He Tian , Yee Brian A. , Loganathan Sampath K. , Liu Lina , Chan Derek C.H. , Tajik Amanda , Tsao Emily , Moreira Steven , Joshi Pratik , Xu Joshua , Wong Nicholas , Balde Zaldy , Jahangiri Soheil , Zandi Sasan , Aigner Stefan , Dick John E. , Minden Mark D. , Schramek Daniel , Yeo Gene W. , Hope Kristin J.
Published: April 2023

Table S6 shows altered gene sets identified by GSEA of the ELAVL1 knockout RN2c RNA-sequencing data set.
Supplementary Table S4 from In Vivo Screening Unveils Pervasive RNA-Binding Protein Dependencies in Leukemic Stem Cells and Identifies ELAVL1 as a Therapeutic Target
Authors: Vujovic Ana , de Rooij Laura , Chahi Ava Keyvani , Chen He Tian , Yee Brian A. , Loganathan Sampath K. , Liu Lina , Chan Derek C.H. , Tajik Amanda , Tsao Emily , Moreira Steven , Joshi Pratik , Xu Joshua , Wong Nicholas , Balde Zaldy , Jahangiri Soheil , Zandi Sasan , Aigner Stefan , Dick John E. , Minden Mark D. , Schramek Daniel , Yeo Gene W. , Hope Kristin J.
Published: April 2023

Table S4 shows limiting dilution analysis of secondary recipients transplanted with DMSO- or MS-444-treated bone marrow from primary mice
Supplementary Table S1 from In Vivo Screening Unveils Pervasive RNA-Binding Protein Dependencies in Leukemic Stem Cells and Identifies ELAVL1 as a Therapeutic Target
Authors: Vujovic Ana , de Rooij Laura , Chahi Ava Keyvani , Chen He Tian , Yee Brian A. , Loganathan Sampath K. , Liu Lina , Chan Derek C.H. , Tajik Amanda , Tsao Emily , Moreira Steven , Joshi Pratik , Xu Joshua , Wong Nicholas , Balde Zaldy , Jahangiri Soheil , Zandi Sasan , Aigner Stefan , Dick John E. , Minden Mark D. , Schramek Daniel , Yeo Gene W. , Hope Kristin J.
Published: April 2023

Table S1 shows expression of RBPs in an RNA-sequencing data set of human bone marrow hematopoietic stem and progenitor subpopulations.
Supplementary Figure S4 from In Vivo Screening Unveils Pervasive RNA-Binding Protein Dependencies in Leukemic Stem Cells and Identifies ELAVL1 as a Therapeutic Target
Authors: Vujovic Ana , de Rooij Laura , Chahi Ava Keyvani , Chen He Tian , Yee Brian A. , Loganathan Sampath K. , Liu Lina , Chan Derek C.H. , Tajik Amanda , Tsao Emily , Moreira Steven , Joshi Pratik , Xu Joshua , Wong Nicholas , Balde Zaldy , Jahangiri Soheil , Zandi Sasan , Aigner Stefan , Dick John E. , Minden Mark D. , Schramek Daniel , Yeo Gene W. , Hope Kristin J.
Published: April 2023

Supplemental Figure S4 shows the validation of ELAVL1 knockdown by western blot and effects of ELAVL1 loss on human primary AML cells in vitro and in vivo.
Supplementary Figure S2 from In Vivo Screening Unveils Pervasive RNA-Binding Protein Dependencies in Leukemic Stem Cells and Identifies ELAVL1 as a Therapeutic Target
Authors: Vujovic Ana , de Rooij Laura , Chahi Ava Keyvani , Chen He Tian , Yee Brian A. , Loganathan Sampath K. , Liu Lina , Chan Derek C.H. , Tajik Amanda , Tsao Emily , Moreira Steven , Joshi Pratik , Xu Joshua , Wong Nicholas , Balde Zaldy , Jahangiri Soheil , Zandi Sasan , Aigner Stefan , Dick John E. , Minden Mark D. , Schramek Daniel , Yeo Gene W. , Hope Kristin J.
Published: April 2023

Supplemental Figure S2 shows validation of sgRNA -mediated knockout of hit RBPs, comparison of hit RBP depletion in RN2c in vitro versus in vivo, effects of ELAVL1 loss on human AML cell lines, characteristics of the MLL-AF9 AML mouse model, and ELAVL1 expression in normal hematopoietic cells.
Supplemental Table 1 from Digital Spatial Profiling Links Beta-2-microglobulin Expression with Immune Checkpoint Blockade Outcomes in Head and Neck Squamous Cell Carcinoma
Authors: Gavrielatou Niki , Vathiotis Ioannis , Aung Thazin Nwe , Shafi Saba , Burela Sneha , Fernandez Aileen I. , Moutafi Myrto , Burtness Barbara , Economopoulou Panagiota , Anastasiou Maria , Foukas Periklis , Psyrri Amanda , Rimm David L.
Published: April 2023

clinical characteristics of discovery (Athens) and validation (Yale) cohorts
Supplemental Figure 2 from Digital Spatial Profiling Links Beta-2-microglobulin Expression with Immune Checkpoint Blockade Outcomes in Head and Neck Squamous Cell Carcinoma
Authors: Gavrielatou Niki , Vathiotis Ioannis , Aung Thazin Nwe , Shafi Saba , Burela Sneha , Fernandez Aileen I. , Moutafi Myrto , Burtness Barbara , Economopoulou Panagiota , Anastasiou Maria , Foukas Periklis , Psyrri Amanda , Rimm David L.
Published: April 2023

b2m expression range, multivariate analysis of pfs and os and association with response and disease control in Athens cohort
Supplemental Figure 1 from Digital Spatial Profiling Links Beta-2-microglobulin Expression with Immune Checkpoint Blockade Outcomes in Head and Neck Squamous Cell Carcinoma
Authors: Gavrielatou Niki , Vathiotis Ioannis , Aung Thazin Nwe , Shafi Saba , Burela Sneha , Fernandez Aileen I. , Moutafi Myrto , Burtness Barbara , Economopoulou Panagiota , Anastasiou Maria , Foukas Periklis , Psyrri Amanda , Rimm David L.
Published: April 2023

signal to noise ratio plots for tumor, leukocyte and macrophage compartments
Sobre Nilio (Micronilio), com descrição de uma espécie nova (Coleoptera, Nilionidae)
Authors: Reichardt Hans
Published: April 2023

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Brazilian Miocene crabs I. Taxonomic review of Cyclocancer tuberculatus Beurlen, 1958 and Hepatella amazonica Beurlen, 1958 (Pancrustacea, Decapoda, Brachyura)
Authors: Lima Daniel , Silva Rafael Costa da , Aguilera Orangel , Pinheiro Allysson Pontes , Santana William
Published: April 2023

In describing Cyclocancer to accommodate his new species C. tuberculatus, Beurlen highlighted that his new genus was a cancroid intermediate in form between Atelecyclus and Cancer, but with enough characters to be placed in a new genus. Examination of C. tuberculatus type material revealed that Cyclocancer is a junior synonym of Hepatus. Therefore, C. tuberculatus is herein transferred into Hepatus as H. beurleni nomen novum because H. tuberculatus (Beurlen, 1958) is a junior homonym of H. tuberculatus Saussure (1858), a junior synonym of Hepatus pudibundus (Herbst, 1785). Miohepatus gen. nov., comb. nov. is proposed here to accommodate Hepatella amazonica Beurlen from the Pirabas Formation, Neogene, Pará, Brazil, based on new morphological evidence from the type material.
Entre imagens e o processo de fazer ver: Pesquisa sobre os desenhos figurativos Mebengokré-Xikrin do Acervo Lux Vidal
Authors: Baumgaertner Mariana Floria
Published: April 2023

Os desenhos Mebengokré-Xikrin, coletados pela antropóloga Lux Vidal entre as décadas de 1970-1980, foram feitos por diferentes pessoas, de diferentes idades, em sua maioria homens que nunca haviam desenhado antes. São produções espontâneas que, continuamente (re)visitadas, evocam narrativas que não seriam possíveis de outro modo. Ao investigar o processo de formação desta coleção, que conta com 431 desenhos figurativos abrigados no Laboratório de Imagem e Som em Antropologia (LISA-USP), procura-se evidenciar como esses desenhos falam sobre a construção da relação entre Lux Vidal e os Xikrin. Estes arquivos são permeados pelo encontro, característica definidora desta coleção. Ao destacar o processo de produção, arquivamento e documentação dos desenhos, procura-se demonstrar a especificidade desta coleção, relacionando-a aos outros arquivos do Acervo Lux Vidal.
Entre tensões, estereótipos e cisões: A identidade japonesa no brasil e sua representatividade na música
Authors: Rodrigues Flávio
Published: April 2023

A trajetória dos imigrantes japoneses no Brasil é marcada por uma série de tensões, estereótipos e cisões entre a própria comunidade acerca de sua identidade. As discussões sobre noções de japonesidade e brasilidade entre descendentes, no entanto, ganham novos contornos e arenas a partir do uso da arte. Neste trabalho, abordaremos aspectos da trajetória japonesa no Brasil, a formação de diferentes concepções e relações de etnicidade e discutiremos exemplos de como cada um destes grupos usa a música como ferramenta de afirmação de nacionalidade e plataforma de diálogo. Ressaltaremos também a divisão política intrínseca a estes movimentos e como as redes sociais têm permitido a emersão de novas vozes ao debate, entrevistando artistas militantes ativas na construção destes espaços.
MEDEA: Experiência em videoperformance
Authors: Lyra Luciana
Published: April 2023

Escrito em primeira pessoa, este texto tem por escopo apresentar a videoperformance MEDEA, com atuação de Luciana Lyra, direção de Ana Cecília Costa para o texto de Newton Moreno. Criada em 2021, a ação videográfica, com 18 minutos de duração, busca inspiração em imagens das xilogravuras alemãs e brasileiras, de tons expressionistas, para dar conta da densa narrativa que se desdobra acerca desta personagem icônica da tragédia grega, aqui revisitada em contexto brasileiro.  Sendo a MEDEA, nesta narrativa videoperformada, uma nordestina na diáspora ao Sudeste nacional, o trabalho acaba por denunciar o estado de opressão sofrido pelas mulheres migrantes, dando contornos feministas à ação artística, signo das atuações e encenações encampadas por Lyra, integrante do NAPEDRA (Núcleo de Antropologia, performance e drama-USP), desde 2004, e fundadora do grupo de pesquisa MOTIM – Mito, Rito e Cartografias Feministas nas Artes (CNPq-UERJ), em 2015.
Batucando-Cantando-Dançando: Saberes corporificados, celebração e contestação política no maracatu em São Paulo
Authors: Santos Kelwin Marques Garcia dos
Published: April 2023

Partindo da discussão sobre a relação entre os recentes grupos e as tradicionais nações de maracatu, este artigo intenta compreender o papel do corpo como fulcro para pensar a criação e transmissão de conhecimentos e memórias. Discute-se também como o maracatu pode se apresentar enquanto complexo político para os coletivos que o geram, e  disputado como instrumento político - enquanto ritmo ou signo de brasilidade - por agentes exógenos a essas comunidades. A partir de uma experiência de campo com o maracatu em uma manifestação política artivista em defesa da democracia, em São Paulo,  2018, propõe-se compreender tal participação enquanto performance geradora de novas localidades.
Uma vida fronteiriça, entre viver e performar: Estudo de caso da performer Sucia Infecciosa Inmunda
Authors: Santos Cristiane de Almeida
Published: April 2023

A performance artística é um ato, ou melhor, uma ação que quase sempre respeita um programa performativo pré-concebido que pensa a duração, local e execução. Contudo, diversos performers extrapolaram o que se entende por programa e vivenciaram ações performativas de longa duração. Mas, e quando a performance e vida se misturam, o que sobra? Como podemos identificar o que é vida e o que é performance? Nesse sentido, existem aqueles que experienciam estar na fronteira entre viver e performar, nesse caso, a fronteira não se constitui como barreira ou separação e sim local de encontros, trocas, ao qual não se sabe onde termina um espaço e começa o outro, é fronteira como entrelugar de fluição da vida. Este artigo trata de Sucia Infecciosa Inmunda, uma mulher trans, performer “monstrificada” pela sociedade e que decide performar a “monstrificação” como forma de (r)existir.
Navicula sinicomeniscus sp. nov. (Bacillariophyta; Naviculaceae), a new species from southwestern China
Authors: XIAO QIAO-ZHI , GUO JI-SHU , TANG ZHENG-BIN , KOCIOLEK JOHN PATRICK , LI YAN-LING
Published: April 2023

Based on light and scanning electron microscopy observations, Navicula sinicomeniscus sp. nov. is described here as a new species from oligotrophic Lake Fuxian in Yunnan Province, China. Characteristics of this new species used to assign it to the genus Navicula include filiform, almost straight to slightly curved raphe structure, lineolate striae, and a row of visible, horizontal areolae positioned on the valve mantle at the apices. The key diagnostic features between the new species and other similar species include: valve shape and size, stria and areola densities, as well as the straightness or degree of bending of the raphe. Comparison with morphologically similar species is discussed in detail. These results can help us further understand the morphological and ecological information of the genus Navicula in Fuxian Lake.
Morphological and molecular evidence support a new truffle, Tuber sinomacrosporum, in Macrosporum group
Authors: WAN SHAN-PING , LIU WEI , CUI MENG-JIN , WANG RUI , WANG YU-YUN , LIU MEI-JU , WANG RUI-XUE , YU CHENG-JIN , YU FU-QIANG
Published: April 2023

A new Chinese truffle species, Tuber sinomacrosporum belongs to the Macrosporum group was illustrated and described, it is characterized by brownish-red ascoma, prosenchymatous peridium, dark brown gleba, 1(2) spored asci and large ellipsoid ascospores with 2–6 μm high reticulate-alveolate. Molecular analysis of the internal transcribed spacers (ITS) of the nuclear ribosomal DNA supports this species is distinct from the known species within the Macrosporum group. Phylogenetic relationships and morphological differences between this new species and its related species are discussed.
A new species Begonia wiformis, and a new record of B. filiformis (B. sect. Coelocentrum, Begoniaceae) from nothern Vietnam
Authors: HOANG THANH SON , LIN CHE-WEI
Published: April 2023

Begonia sect. Coelocentrum is a highly diverse section of the genus (87 species) with a Sino-Vietnamese distribution. A new species, Begonia wiformis, is described and illustrated herein, it is characterized by its rhizomes that send out elongated stolons, subglabrous lamina with a velvety texture adaxially, bracts with a denticulate margin, and a zygomorphic androecium. Additionally, Begonia filiformis, a species previously known only from China, is reported for the first time in Vietnam.
Rediscovery of Gryllomorpha miramae Medvedev, 1933 (Orthoptera: Gryllidae: Gryllomorphinae) in Ukraine
Authors: MARTYNOV ALEXANDER V.
Published: April 2023

Gryllomorpha miramae Medvedev, 1933 was collected in Zaporizhzhia and Kherson Regions of Ukraine. These are the first records of the species within Ukraine since more than 60 years from previous (and the first) record of the species within Zaporizhzhia Region. The species was found within intact and damaged steppe habitats. Our investigation shows that middle-age larvae demonstrate typical for the species coloration and shape of denticles of outer surface of hind tibia. This contribution provides first COI sequence for G. miramae.
A taxonomic reassessment of Cirrodrilus japonicus (Pierantoni, 1912) (Annelida, Clitellata, Branchiobdellida)
Authors: OHTAKA AKIFUMI , GELDER STUART R.
Published: April 2023

Cirrodrilus japonicus (Pierantoni, 1912) has not been identified since it was originally described from specimens removed from preserved Japanese crayfish that had been deposited in the Natural History Museum in Hamburg, Germany. A morphological comparison of the published description and recently discovered syntypes of C. japonicus from the Hamburg Museum with syntypes and newly collected material of Cirrodrilus ezoensis (Yamaguchi, 1934), supported the view that preservation artifacts in C. japonicus caused Prof. Yamaguchi to believe his well-preserved specimens were member of a separate species. Therefore, C. ezoensis becomes a junior synonym of C. japonicus.
Suicide and Self-Harm Among Immigrant Youth to Ontario, Canada From Muslim Majority Countries: A Population-Based Study
Authors: Saunders Natasha , Strauss Rachel , Swayze Sarah , Kopp Alex , Kurdyak Paul , Furqan Zainab , Malick Arfeen , Husain Muhammad Ishrat , Sinyor Mark , Zaheer Juveria
Published: April 2023

Objective

To examine the association between Muslim religious affiliation and suicide and self-harm presentations among first- and second-generation immigrant youth.

Methods

We performed a population-based cohort study involving individuals aged 12 to 24 years, living in Ontario, who immigrated to Canada between 1 January 2003 and 31 May 2017 (first generation) and those born to immigrant mothers (second generation). Health administrative and demographic data were used to analyze suicide and self-harm presentations. Sex-stratified logistic regression models generated odds ratios (OR) for suicide and negative binomial regression models generated rate ratios (aRR) for self-harm presentations, adjusting for refugee status and time since migration.

Results

Of 1,070,248 immigrant youth (50.1% female), there were 129,919 (23.8%) females and 129,446 (24.2%) males from Muslim-majority countries. Males from Muslim-majority countries had lower suicide rates (3.8/100,000 person years [PY]) compared to males from Muslim-minority countries (5.9/100,000 PY) (OR: 0.62, 95% CI, 0.42–0.92). Rates of suicide between female Muslim-majority and Muslim-minority groups were not different (Muslim-majority 1.8/100,000 PY; Muslim-minority 2.2/100,000 PY) (OR: 0.82, 95% CI, 0.46–1.47). Males from Muslim-majority countries had lower rates of self-harm presentations than males from Muslim-minority (<10%) countries (Muslim majority: 12.2/10,000 PY, Muslim-minority: 14.1/10,000 PY) (aRR: 0.82, 95% CI, 0.75, 0.90). Among female immigrants, rates of self-harm presentations were not different among Muslim-majority (30.1/10,000 PY) compared to Muslim-minority (<10%) (32.9/10,000 PY) (aRR: 0.93, 95% CI, 0.87–1.00) countries. For females, older age at immigration conferred a lower risk of self-harm presentations.

Conclusion

Being a male from a Muslim-majority country may confer protection from suicide and self-harm presentations but the same was not observed for females. Approaches to understanding the observed sex-based differences are warranted.
Exploration of tolerance of unfairness under COVID-19 mortality salience and its effect on epidemic development
Authors: Peng Lin , Luo Siyang
Published: April 2023

COVID-19 has brought awareness of the daily threat of death to everyone in the world and provided a natural context for raising widespread awareness of the salience of mortality. Previous researchers have found that mortality salience has rendered proposers more likely to make a fair offer in the dictator and ultimatum game, but there has been no study focusing on the psychological changes in the responders. Study 1 was an exploratory study of the effect of mortality salience on the threshold for acceptance of unfair offers, comparing the effect of unnatural deaths, such as those caused by COVID-19, and that of natural deaths, such as those caused by aging. The results showed that COVID-19 mortality salience could lower the acceptance threshold in responders, thus increasing their tolerance of unfairness, while the mortality salience from aging would not. In Study 2, we established an evolutionary game model to simulate the influences of tolerance of unfairness in allocation of resources on epidemic spread using agent-based modeling. The study compared two societies with different levels of the fear of death, and the results showed that the society with a relatively high death fear would produce more inequality in distribution by increasing the tolerance of unfairness. This ultimately leads to worse pandemic conditions and slower control of the spread in the first stage of the pandemic.
Integrated Microbiome and Metabolomic Analysis Reveal Responses of Rhizosphere Bacterial Communities and Root exudate Composition to Drought and Genotype in Rice (Oryza sativa L.)
Authors: Li Gege , Wang Kexin , Qin Qun , Li Qi , Mo Fei , Nangia Vinay , Liu Yang
Published: April 2023

Background

As climate change events become more frequent, drought is an increasing threat to agricultural production and food security. Crop rhizosphere microbiome and root exudates are critical regulators for drought adaptation, yet our understanding on the rhizosphere bacterial communities and root exudate composition as affected by drought stress is far from complete. In this study, we performed 16S rRNA gene amplicon sequencing and widely targeted metabolomic analysis of rhizosphere soil and root exudates from two contrasting rice genotypes (Nipponbare and Luodao 998) exposed to drought stress.

Results

A reduction in plant phenotypes was observed under drought, and the inhibition was greater for roots than for shoots. Additionally, drought exerted a negligible effect on the alpha diversity of rhizosphere bacterial communities, but obviously altered their composition. In particular, drought led to a significant enrichment of Actinobacteria but a decrease in Firmicutes. We also found that abscisic acid in root exudates was clearly higher under drought, whereas lower jasmonic acid and L-cystine concentrations. As for plant genotypes, variations in plant traits of the drought-tolerant genotype Luodao 998 after drought were smaller than those of Nipponbare. Interestingly, drought triggered an increase in Bacillus, as well as an upregulation of most organic acids and a downregulation of all amino acids in Luodao 998. Notably, both Procrustes analysis and Mantel test demonstrated that rhizosphere microbiome and root exudate metabolomic profiles were highly correlated. A number of differentially abundant genera responded to drought and genotype, including Streptomyces, Bacillus and some members of Actinobacteria, were significantly associated with organic acid and amino acid contents in root exudates. Further soil incubation experiments showed that Streptomyces was regulated by abscisic acid and jasmonic acid under drought.

Conclusions

Our results reveal that both drought and genotype drive changes in the compositions of rice rhizosphere bacterial communities and root exudates under the greenhouse condition, and that organic acid exudation and suppression of amino acid exudation to select specific rhizosphere bacterial communities may be an important strategy for rice to cope with drought. These findings have important implications for improving the adaptability of rice to drought from the perspective of plant–microbe interactions.
Identification of immune-related molecular markers in intracranial aneurysm (IA) based on machine learning and cytoscape-cytohubba plug-in
Authors: Ma Zhengfei , Zhong Ping , Yue Peidong , Sun Zhongwu
Published: April 2023

Background

Intracranial aneurysm (IA) is a common cerebrovascular disease. The immune mechanism of IA is more complicated, and it is unclear so far. Therefore, it is necessary to continue to explore the immune related molecular mechanism of IA.

Methods

All data were downloaded from the public database. Limma package and ssGSEA algorithm was used to identify differentially expressed mRNAs (DEmRNAs) and analyze immune cell infiltration, respectively. Machine learning and cytoscape-cytohubba plug-in was used to identify key immune types and multicentric DEmRNAs of IA, respectively. Multicentric DEmRNAs related to key immune cells were screened out as key DEmRNAs by Spearman correlation analysis. Diagnostic models, competing endogenous RNA (ceRNA) regulatory network and transcription factor regulatory network were constructed based on key DEmRNAs. Meanwhile, drugs related to key DEmRNAs were screened out based on DGIdb database. The expression of key DEmRNAs was also verified by real time-PCR.

Results

In this study, 7 key DEmRNAs (NRXN1, GRIA2, SLC1A2, SLC17A7, IL6, VEGFA and SYP) associated with key differential immune cell infiltration (CD56bright natural killer cell, Immature B cell and Type 1 T helper cell) were identified. Functional enrichment analysis showed that VEGFA and IL6 may be involved in the regulation of the PI3K-Akt signaling pathway. Moreover, IL6 was also found to be enriched in cytokine-cytokine receptor interaction signaling pathway. In the ceRNA regulatory network, a large number of miRNAs and lncRNAs were found. In the transcription factor regulatory network, the transcription factor SP1 was correlated with VEGFA, SYP and IL6. It is also predicted that drugs related to key DEmRNAs such as CARBOPLATIN, FENTANYL and CILOSTAZOL may contribute to the treatment of IA. In addition, it was also found that SVM and RF models based on key DEmRNAs may be potential markers for diagnosing IA and unruptured intracranial aneurysm (UIA), respectively. The expression trend of key DEmRNAs verified by real-time PCR was consistent with the bioinformatics analysis results.

Conclusion

The identification of molecules and pathways in this study provides a theoretical basis for understanding the immune related molecular mechanism of IA. Meanwhile, the drug prediction and diagnosis model construction may also be helpful for clinical diagnosis and management.