bluuebunny/crossref_metadata_2025 huggingface dataset:
Premorbid Psychological Factors Associated with Long-Term Postoperative Headache after Microsurgery in Vestibular Schwannoma—A Retrospective Pilot Study
Authors: Thomas Mareike , Rampp Stefan , Scheer Maximilian , Strauss Christian , Prell Julian , Schönfeld Robby , Leplow Bernd
Published: August 2023

Associations between premorbid psychological factors and postoperative headache (POH) after microsurgical treatment via the retrosigmoid approach for vestibular schwannoma (VS) were investigated in this retrospective single-center study. A total of 101 VS patients completed the Rostock headache questionnaire (RoKoKo), the hospital and anxiety scale (HADS-D), and the screening for somatoform disorders (SOMS-2), all of which were used as short self-assessed questionnaires. Fifty-four patients with POH were compared with 47 non-POH patients in terms of premorbid psychological factors, somatization tendencies, and psychological burden using the chi2-test and Mann–Whitney U-test. Regression analyses were conducted to assess the weighted contribution of psychological and procedural factors to POH. In individuals with POH, mental ailments, preexisting headaches, premorbid chronic pain syndromes, and higher somatization tendencies were found to be significantly more common. POH was predicted by the number of premorbid psychosomatic symptoms, preexisting mental ailments, and premorbid chronic pain syndromes. Depression and anxiety were predicted by low emotional stability. Additionally, the number of premorbid psychosomatic symptoms predicted depression, anxiety, and overall psychological burden. It was observed that the reported symptoms of headache might fit into the classification of chronic postsurgical pain (CPSP) rather than being classified as secondary headaches after craniotomy. Premorbid psychological factors were found to play an important role in the emergence of POH in VS, particularly after microsurgery via the retrosigmoid approach. Therefore, it is suggested that psychological screening be incorporated into the treatment process.
Association between Wind Environment and Spatial Characteristics of High-Rise Residential Buildings in Cold Regions through Field Measurements in Xi’an
Authors: Zhang Qian , Zhou Dian , Xu Duo , Rogora Alessandro
Published: August 2023

Research on the correlation between wind and block spatial form focuses mainly on hot and humid cities. However, cold regions are also experiencing high summer temperatures due to global climate change. Enhancing wind speed in blocks through urban spatial control improves comfort. Existing research cannot be directly applied to cold regions due to natural differences. Using Xi’an as an example, this study explores the impact of high-rise residential block spatial form on internal and external wind environments through field measurements and simulations. Optimal strategies for block planning and architectural design are identified to improve the wind environment. Results show that blocks with high buildings on the south and north sides and low buildings in the middle achieve a more comfortable internal wind environment. Gradually increasing building height from south to north has minimal impact on downwind blocks. Reducing the angle between the main facade and dominant wind direction enhances the residential area’s wind environment. Specific spatial planning and design strategies are summarized for early-stage decision-making.
Rethinking Urban Decline in Post-COVID19: Bibliometric Analysis and Countermeasures
Authors: Zhang Jiazhen , Yao Muxia , Cenci Jeremy
Published: August 2023

Urban decline refers to the sustained deterioration of cities in terms of their economy, population, and social aspects. The outbreak of the Coronavirus Disease 2019 (COVID-19) pandemic in 2019 objectively affected the trajectory of this phenomenon. A comprehensive analysis of scientific research on urban decline and its practical implications was conducted using bibliometric methods, data acquired from 2019 to 2023 and the Web of Science. Since COVID-19, research on urban decline has been predominantly led by traditional developed countries such as the United States and England, with a high degree of regional collaboration. Keyword clusters have focused on urban regeneration, growth, decay, family planning, resource dependency theory, public art, etc. Keyword co-occurrence has focused on shrinking cities, gentrification policy, land use, etc. Based on previous analyses and the contemporary context, the intrinsic logic behind the urban decline in recent years can be summarized as inadequate economic development, lagging infrastructure construction, the siphoning effect of core regional cities, and unique institutional factors leading to specific urban decline patterns. Comprehensive urban recovery plans have been proposed, including reshaping urban spatial layouts and planning and strengthening strategies for social and economic revival, with correspondence-specific samples. Studying the impact of COVID-19 on urban decline from the perspectives of city development and strategies can help us better understand the repercussions of global health crises on cities, providing a more scientific basis for urban planning and management to build resilient, sustainable, and equitable cities.
Deep Transfer Learning with Enhanced Feature Fusion for Detection of Abnormalities in X-ray Images
Authors: Alammar Zaenab , Alzubaidi Laith , Zhang Jinglan , Li Yuefeng , Lafta Waail , Gu Yuantong
Published: August 2023

Medical image classification poses significant challenges in real-world scenarios. One major obstacle is the scarcity of labelled training data, which hampers the performance of image-classification algorithms and generalisation. Gathering sufficient labelled data is often difficult and time-consuming in the medical domain, but deep learning (DL) has shown remarkable performance, although it typically requires a large amount of labelled data to achieve optimal results. Transfer learning (TL) has played a pivotal role in reducing the time, cost, and need for a large number of labelled images. This paper presents a novel TL approach that aims to overcome the limitations and disadvantages of TL that are characteristic of an ImageNet dataset, which belongs to a different domain. Our proposed TL approach involves training DL models on numerous medical images that are similar to the target dataset. These models were then fine-tuned using a small set of annotated medical images to leverage the knowledge gained from the pre-training phase. We specifically focused on medical X-ray imaging scenarios that involve the humerus and wrist from the musculoskeletal radiographs (MURA) dataset. Both of these tasks face significant challenges regarding accurate classification. The models trained with the proposed TL were used to extract features and were subsequently fused to train several machine learning (ML) classifiers. We combined these diverse features to represent various relevant characteristics in a comprehensive way. Through extensive evaluation, our proposed TL and feature-fusion approach using ML classifiers achieved remarkable results. For the classification of the humerus, we achieved an accuracy of 87.85%, an F1-score of 87.63%, and a Cohen’s Kappa coefficient of 75.69%. For wrist classification, our approach achieved an accuracy of 85.58%, an F1-score of 82.70%, and a Cohen’s Kappa coefficient of 70.46%. The results demonstrated that the models trained using our proposed TL approach outperformed those trained with ImageNet TL. We employed visualisation techniques to further validate these findings, including a gradient-based class activation heat map (Grad-CAM) and locally interpretable model-independent explanations (LIME). These visualisation tools provided additional evidence to support the superior accuracy of models trained with our proposed TL approach compared to those trained with ImageNet TL. Furthermore, our proposed TL approach exhibited greater robustness in various experiments compared to ImageNet TL. Importantly, the proposed TL approach and the feature-fusion technique are not limited to specific tasks. They can be applied to various medical image applications, thus extending their utility and potential impact. To demonstrate the concept of reusability, a computed tomography (CT) case was adopted. The results obtained from the proposed method showed improvements.
Biogenic Silver Nanoparticles for Targeted Cancer Therapy and Enhancing Photodynamic Therapy
Authors: Kah Glory , Chandran Rahul , Abrahamse Heidi
Published: August 2023

Different conventional therapeutic procedures are utilized globally to manage cancer cases, yet the mortality rate in patients with cancer remains considerably high. Developments in the field of nanotechnology have included novel therapeutic strategies to deal with cancer. Biogenic (green) metallic silver nanoparticles (AgNPs) obtained using plant-mediated protocols are attractive to researchers exploring cancer treatment. Biogenic AgNPs present advantages, since they are cost-effective, easy to obtain, energy efficient, and less toxic compared to chemically and physically obtained AgNPs. Also, they present excellent anticancer abilities thanks to their unique sizes, shapes, and optical properties. This review provides recent advancements in exploring biogenic AgNPs as a drug or agent for cancer treatment. Thus, great attention was paid to the anticancer efficacy of biogenic AgNPs, their anticancer mechanisms, their efficacy in cancer photodynamic therapy (PDT), their efficacy in targeted cancer therapy, and their toxicity.
Current and Future Landscape in Genetic Therapies for Leber Hereditary Optic Neuropathy
Authors: Shamsnajafabadi Hoda , MacLaren Robert E. , Cehajic-Kapetanovic Jasmina
Published: August 2023

Leber hereditary optic neuropathy (LHON) is the most common primary mitochondrial genetic disease that causes blindness in young adults. Over 50 inherited mitochondrial DNA (mtDNA) variations are associated with LHON; however, more than 95% of cases are caused by one of three missense variations (m.11778 G > A, m.3460 G > A, and m.14484 T > C) encoding for subunits ND4, ND1, and ND6 of the respiration complex I, respectively. These variants remain silent until further and currently poorly understood genetic and environmental factors precipitate the visual loss. The clinical course that ensues is variable, and a convincing treatment for LHON has yet to emerge. In 2015, an antioxidant idebenone (Raxone) received European marketing authorisation to treat visual impairment in patients with LHON, and since then it was introduced into clinical practice in several European countries. Alternative therapeutic strategies, including gene therapy and gene editing, antioxidant and neurotrophic agents, mitochondrial biogenesis, mitochondrial replacement, and stem cell therapies are being investigated in how effective they might be in altering the course of the disease. Allotopic gene therapies are in the most advanced stage of development (phase III clinical trials) whilst most other agents are in phase I or II trials or at pre-clinical stages. This manuscript discusses the phenotype and genotype of the LHON disease with complexities and peculiarities such as incomplete penetrance and gender bias, which have challenged the therapies in development emphasising the most recent use of gene therapy. Furthermore, we review the latest results of the three clinical trials based on adeno-associated viral (AAV) vector-mediated delivery of NADH dehydrogenase subunit 4 (ND4) with mitochondrial targeting sequence, highlighting the differences in the vector design and the rationale behind their use in the allotopic transfer.
Nanogels Based on Hyaluronic Acid as Potential Active Carriers for Dermatological and Cosmetic Applications
Authors: Mauri Emanuele , Scialla Stefano
Published: August 2023

Nanogels are a prominent research topic in biomedical and drug delivery applications. The versatility of their chemistry allows them to be tailored both to carry and release a wide range of active molecules, and to target specific tissues or cell types. Within a vast field of possible chemical designs, nanogels based on hyaluronic acid seem particularly interesting from the standpoint of dermatological and cosmetic applications, due to the well-known involvement of hyaluronic acid in several fundamental processes related to skin health and ageing. In spite of this, relatively few studies about these nanocarriers and their potential skin-related benefits have appeared so far in the literature. With the aim to stimulate further interest in the topic, in this review, we provide information on hyaluronic acid-based nanogels, including their key physicochemical properties, their typical drug release behavior, and the main synthetic methodologies. The latter include: approaches based on spontaneous self-assembly of polymer molecules; approaches based on chemical cross-linking, where nanogel formation is promoted by covalent bonds between polymer chains; and hybrid approaches that leverage a combination of the above two mechanisms. We believe this body of information, which we collected by going through the relevant literature from the past 10–15 years, offers cosmetic formulators plenty of options to design innovative products.
Mechanical Characterization and Microstructural Analysis of Stir-Cast Aluminum Matrix Composites (LM5/ZrO2)
Authors: Prakash Jayavelu , Jebarose Juliyana Sunder , Salunkhe Sachin , Gawade Sharad , Nasr Emad , Kamrani Ali
Published: August 2023

Aluminum matrix composites (AMCs) are largely used in defense, maritime, and space applications for their excellent properties. LM5 is used where very high resistance to corrosion from seawater or marine atmospheres is required, for equipment used for the manufacture of foodstuffs, cooking utensils, and chemical plants. Zirconia is preferred over other reinforcements as it shows comparatively great refractory properties, high scratch resistance, and thermal shock resistance. Utilizing the stir casting technique, an attempt was made to produce AMCs of LM5 aluminum alloy strengthened with ZrO2. The weight percentage of ZrO2 was changed to 0%, 3%, 6%, and 9%. The specimens were prepared and tested as per ASTM standards to find the density, micro and macro hardness, impact, tensile, and compressive strength. The micrographs and SEM images confirm the uniform distribution of ZrO2 particles in the aluminum matrix. LM5/9%ZrO2 AMC has the highest density value of 2.83 g/cm3 and LM5/3%ZrO2 has the least porosity of 2.55%. LM5/9% ZrO2 has the highest hardness values of 78 VHN and 72 HRE. LM5/6% ZrO2 AMC has the highest tensile strength of 220 MPa, compressive strength of 296 MPa, and toughness of 12 J. LM5/6% ZrO2 AMCs may be used for many structural applications.
Asymmetric Measurement-Device-Independent Quantum Key Distribution through Advantage Distillation
Authors: Zhang Kailu , Liu Jingyang , Ding Huajian , Zhou Xingyu , Zhang Chunhui , Wang Qin
Published: August 2023

Measurement-device-independent quantum key distribution (MDI-QKD) completely closes the security loopholes caused by the imperfection of devices at the detection terminal. Commonly, a symmetric MDI-QKD model is widely used in simulations and experiments. This scenario is far from a real quantum network, where the losses of channels connecting each user are quite different. To adapt such a feature, an asymmetric MDI-QKD model is proposed. How to improve the performance of asymmetric MDI-QKD also becomes an important research direction. In this work, an advantage distillation (AD) method is applied to further improve the performance of asymmetric MDI-QKD without changing the original system structure. Simulation results show that the AD method can improve the secret key rate and transmission distance, especially in the highly asymmetric cases. Therefore, this scheme will greatly promote the development of future MDI-QKD networks.
Time Series Forest Fire Prediction Based on Improved Transformer
Authors: Miao Xinyu , Li Jian , Mu Yunjie , He Cheng , Ma Yunfei , Chen Jie , Wei Wentao , Gao Demin
Published: August 2023

Forest fires, severe natural disasters causing substantial damage, necessitate accurate predictive modeling to guide preventative measures effectively. This study introduces an enhanced window-based Transformer time series forecasting model aimed at improving the precision of forest fire predictions. Leveraging time series data from 2020 to 2021 in Chongli, a myriad of forest fire influencing factors were ascertained using remote sensing satellite and GIS technologies, with their interrelationships estimated through a multicollinearity test. Given the intricate nature of real-world forest fire prediction tasks, we propose a novel window-based Transformer architecture complemented by a dual time series input strategy premised on 13 influential factors. Subsequently, time series data were incorporated into the model to generate a forest fire risk prediction map in Chongli District. The model’s effectiveness was then evaluated using various metrics, including accuracy (ACC), root mean square error (RMSE), and mean absolute error (MAE), and compared with traditional deep learning methods. Our model demonstrated superior predictive performance (ACC = 91.56%, RMSE = 0.37, MAE = 0.05), harnessing spatial background information efficiently and effectively utilizing the periodicity of forest fire factors. Consequently, the study proves this method to be a novel and potent approach for time series fire prediction.
Effect of Condensed Tannins on Nitrogen Distribution and Metabolome after Aerobic Exposure of Sainfoin Silage
Authors: Huang Rongzheng , Zhang Fanfan , Wang Xuzhe , Ma Chunhui
Published: August 2023

(1) Background: Previous studies have indicated that proteolysis is inhibited by the condensed tannins (CTs) that are present during sainfoin ensiling. Whether inhibiting this effect of CTs on proteolysis is functional during aerobic exposure is still unclear. (2) Methods: the present study investigated the effect of CTs on metabolite composition during the aerobic exposure of sainfoin silage via the use of polyethylene glycol (PEG), leading to the inactivation of CTs. (3) Results: The neutral detergent-insoluble protein (NDIP) and acid detergent-insoluble protein concentrations were both more concentrated in the control group than in the PEG-treated group. There were 587 and 651 different metabolites present in the control and PEG-treated groups after 3 and 7 days, respectively, of aerobic exposure of silage. Flavonoids (72 metabolites) were the most abundant among these different metabolites. The addition of PEG upregulated histidine, threonine, asparagine, tryptophan, and glutamine, but downregulated phenylalanine. The relative abundances of Lactococcus, Fructobacillus, Enterobacter, Cutibacterium, Citrobacter, and Rosenbergiella differed significantly between the control and PEG-treated groups (p < 0.05); all of these bacteria showed significant correlation with some of the 50 most abundant metabolites. (4) Conclusions: the results suggest that the antioxidant status of the silage increased and inhibited the activity of a variety of bacteria that coexist with CTs, and decreased the production of certain amino acids after the aerobic exposure of silage.
Impact of Lactobacillus acidophilus—La5 on Composition and Metabolism of the Intestinal Microbiota of Type 2 Diabetics (T2D) and Healthy Individuals Using a Microbiome Model
Authors: Salgaço Mateus Kawata , de Oliveira Fellipe Lopes , Sartoratto Adilson , Mesa Victoria , Mayer Marcia Pinto Alves , Sivieri Katia
Published: August 2023

Type 2 diabetes is characterized by dysbiosis in the gut, which may lead to systemic inflammation. Therefore, the use of probiotics may help to achieve a balanced microbiota and improve glycemic control. The aim of this study was to verify the impact of Lactobacillus acidophilus—La5 on the gut microbiome of type 2 diabetes adults using the Human Gut Microbial Ecosystem Simulator (SHIME®) and compare this to the microbiome of healthy subjects. Four groups (Control Group: NormoGlycemic; Treatment Group: T2D) were evaluated in SHIME® for 6 weeks. After 7 and 14 days of colonic fermentation, the intestinal microbiota (16S rRNA gene sequencing) and metabolites (short-chain fatty acids) were analyzed. La5 altered the composition of the microbiota after 14 days of treatment for both groups, by increasing the abundance of Bacteroidetes and a decrease in Firmicutes in the NormoGlycemic. Treatment with La5 resulted in a shift in the microbial community of NormoGlycemic with increased abundance of Bacteroides and Mitsuokella and a decrease in Achromobacter and Catabacter, whereas T2D gut microbiome was enriched with Faecalibacterium and reduced in Bacteroides. Megasphaera spp. stimulated with La5 treatment in NormoGlycemic has already been reported to produce intestinal metabolites and recognized to contribute to increased anti-inflammatory and immune responses. Faecalibacterium, on the other hand, can modulate the intestinal epithelium and be a major butyrate product in the microbiota. Finally, this study showed a positive and promising result of La5 treatment in increasing intestinal homeostasis in the microbiota of T2D.
Beef Cattle Grazing Native Grasslands May Follow Three Different Supplement Response Patterns
Authors: Cazzuli Fiorella , Durante Martín , Hirigoyen Andrés , Sánchez Javier , Rovira Pablo , Beretta Virginia , Simeone Alvaro , Jaurena Martín , Savian Jean Victor , Poppi Dennis , Montossi Fabio , Lagomarsino Ximena , Luzardo Santiago , Brito Gustavo , Velazco José Ignacio , Lattanzi Fernando Alfredo , Bremm Carolina
Published: August 2023

Previous studies on winter supplementation of growing cattle grazing stockpiled native Campos grasslands suggest that forage allowance (FA), herbage mass, and weather conditions before and during the supplementation period could all affect supplement feed efficiency (SFE)—that is, the difference or change in average daily gain (ADG) between supplemented (S) and control (C) animals (ADGchng, kg) per unit (kg) of supplement dry matter (DM) intake. In this study, we analyse data from fifteen collated winter supplementation trials carried out in Uruguay between 2004 and 2018. The working hypotheses of this research paper were: (i) that average substitution rates are positive, and (ii) that ADGchng is not constant throughout the supplementation period and that its variation may be attributed to sward, animal or weather variables. There were two main objectives: (i) to estimate the average supplement substitution rate (sSbR, kg forage, f, dry matter, DM intake reduction: kg supplement DM intake) and potential herbage intake substitution rate (hSbR, kg fDM intake reduction: kg fDM intake of control animals), and its association with SFE, and, (ii) to assess the existence of different phases and supplementation response patterns and its association with other relevant variables. Estimated substitution rates were always positive (sSbR = 0.3–1.1 kg/kg; hSbR = 0.1–0.3 kg/kg) and were negatively and moderately associated with SFE. Supplementation proved to be a dynamic process where three possible supplementation responses over the supplementation period were identified (linear, quadratic and Weibull). While linear patterns did not appear distinctly associated with any particular set of variables, quadratic models were mostly associated with herbage biomass and substitution rates, whereas Weibull models were the clearest in their association with frosts. Regardless of the response pattern, at the beginning of the trials it was the animals’ body weight and supplement quality that most influenced supplement response, whereas towards the end, supplementation intake, supplemented animals’ ADG and forage quality played a more relevant role. The estimated parameters and response patterns are expected to be used as inputs in decision support systems for livestock farmers in the future.
Towards Cultural Adequacy of Experience-Based Design: A Qualitative Evaluation of Community-Integrated Intermediary Care to Enhance the Family-Based Long-Term Care for Thai Older Adults
Authors: Aung Thin Nyein Nyein , Lorga Thaworn , Moolphate Saiyud , Koyanagi Yuka , Angkurawaranon Chaisiri , Supakankunti Siripen , Yuasa Motoyuki , Aung Myo Nyein
Published: August 2023

In this qualitative study, we provided an in-depth understanding of how Community-Integrated Intermediary Care (CIIC), a new service model for family-based long-term care (LTC), was perceived by its users. The CIIC, established in Chiang Mai, Northern Thailand, consisted of three main interventions: (1) A temporary respite care center; (2) A family-centered care capacity building; (3) Functional training delivered as community group exercise and home exercise to improve healthy ageing for independent older adults. Ten pairs of dependent Thai older adults, their primary family caregivers, and ten village health volunteers were recruited using the purposive sampling method. Data were collected via semistructured in-depth interviews. A thematic descriptive qualitative analysis was used for data analysis. The findings revealed that CIIC helped reduce the burden of family caregivers by providing respite, relief, and care coordination. The experiences of the CIIC users indicated possibilities for service redesign, development, and delivery strategies to better meet the LTC needs of older adults and family caregivers. Following the local stakeholders’ commitment and local community health volunteers’ network, a well-integrated formal and informal care CIIC model can be implied as an effective and sustainable ageing care service model in Thailand and other Asian countries in the future.
The Global Prevalence and Associated Factors of Burnout among Emergency Department Healthcare Workers and the Impact of the COVID-19 Pandemic: A Systematic Review and Meta-Analysis
Authors: Alanazy Ahmed Ramdan M. , Alruwaili Abdullah
Published: August 2023

Background/Aim: Emergency medicine (EM) settings are very stressful, given the high workload, intense working environment, and prolonged working time. In turn, the rate of burnout and its three domains have been increasingly reported among healthcare workers (HCWs). Therefore, we conducted this meta-analysis to determine the prevalence and risk factors of burnout among EM HCWs. Methods: Six databases were searched in February 2023, yielding 29 articles (16,619 EM HCWs) reporting burnout or its three domains (emotional exhaustion “EE”, depersonalization “DP”, and personal accomplishment “PA”). The primary outcome was the prevalence of burnout and its domains, while secondary outcomes included the risk factors of high burnout, EE, DP, or low PA. Burnout rates were pooled across studies using STATA software. The prevalence was measured using the pooled effect size (ES), and the random-effects model was used when heterogeneity was encountered; otherwise, the fixed-effects model was used. Results: The prevalence of overall burnout was high (43%), with 35% of EM HCWs having a high risk of burnout. Meanwhile, 39%, 43%, and 36% of EM workers reported having high levels of EE and DP and low levels of PA, respectively. Country-specific changes in the rate of burnout were observed. The rate of high burnout, high EE, high DP, and low PA was higher during the COVID-19 pandemic as compared to the pre-pandemic period. The type of profession (nurses, physicians, residents, etc.) played a significant role in modifying the rate of burnout and its domains. However, gender was not a significant determinant of high burnout or its domains among EM workers. Conclusions: Burnout is a prevalent problem in emergency medicine practice, affecting all workers. As residents progress through their training years, their likelihood of experiencing burnout intensifies. Nurses are most affected by this problem, followed by physicians. Country-associated differences in burnout and its domains are evident.
Integrative Effect of UV-B and Some Organic Amendments on Growth, Phenolic and Flavonoid Compounds, and Antioxidant Activity of Basil (Ocimum basilicum L.) Plants
Authors: Santin Marco , Becagli Michelangelo , Sciampagna Maria Calogera , Mannucci Alessia , Ranieri Annamaria , Castagna Antonella
Published: August 2023

The application of organic amendments, biochar, and wood distillate (WD), as well as the exposure to UV-B radiation, are two sustainable ways to enhance soil fertility and increase plant nutraceutical quality, respectively. However, they have always been studied separately, without testing the eventual synergistic or antagonistic effect when applied together. The present study investigated the effects of biochar (2% w/w), WD (1:100), and their combination (BWD) on some biometric and biochemical parameters of basil plants (Ocimum basilicum L.) exposed to different doses of UV-B radiation (0, 1, 2 h d−1; UV-B irradiance of 1.36 W m−2) in controlled conditions. Root and stem length and weight were not affected by soil amendments, while 1 h d−1 UV-B increased the length (+28%) and weight (+62%) of the aerial part. When combining the above- and below-ground factors, a decrease in root length was observed in the 2 h d−1 UV-B-treated plants in both WD (−36%) and BWD (−39%) treatments. The co-application of below- and above-ground treatments generally decreased phenolic and flavonoid concentration in both fully expanded leaves and vegetative shoot apices. This preliminary study highlights an antagonistic action of the combination of the investigated factors, at these doses, on the plant growth and metabolism that should be considered.
The Biotechnological “Provence” of the Future Provided by Antisense Oligoilators and Olinscides for Horticulturae
Authors: Oberemok Volodymyr V. , Puzanova Yelizaveta V. , Novikov Ilya A.
Published: August 2023

Successful management of the synthesis of secondary metabolites of essential oil plants is the basis for the economic growth of the essential oil industry. Against the backdrop of a growing global population and a decrease in land available for cultivation, simple and effective ways to increase the content of certain components in essential oils are becoming increasingly important. Selection is no longer keeping pace with market needs, which stimulates the search for faster methods to control the biosynthesis of secondary metabolites. In this article, using the genera Lavandula and Mentha as examples, we consider the prospects for use of antisense oligonucleotides (ASO), oligoilators, to rapidly increase the concentration of valuable components in essential oil and its yield. This article discusses the use of unmodified ASOs as regulators of a plant’s secondary metabolism to increase the synthesis of individual valuable components, presenting a completely new way to increase the yield of valuable substances based on unique nucleotide sequences. The proposed approach is effective, affordable, safe, and significantly reduces the time needed to obtain plants that synthesize the required concentrations of target substances. Oligoilators can be used with oligonucleotide insecticides (olinscides) in complex formulations used for green agriculture. Further investigation is needed to determine maximum economic efficiency for this approach.
Impact of Parental Mental Health and Help-Seeking on Adolescents’ Suicidal Ideations and Help-Seeking Behaviors
Authors: Han Jaehyun , Ahn Joung-Sook , Kim Min-Hyuk , Chang Sei-Jin , Kim Jong-Koo , Min Seongho
Published: August 2023

This study aimed to evaluate the impact of parental mental health on adolescent offspring. Data regarding 6512 families from the 2010–2021 Korean National Health and Nutrition Examination Survey were analyzed; among them, 428 were placed in the suicidal ideation (SI) group and 421 were placed in the matched control (MC) group. This number was selected for the use of the propensity score matching method. The findings highlighted significant associations between parental mental health and adolescent suicidal ideation, with mothers in the SI group having higher odds of Diagnosed Depression (OR 2.109, 1.023–4.350 95% CI), Depressive Mood (OR 2.155, 1.224–3.793 95% CI), and Suicidal Ideation (OR 2.532, 1.322–4.851 95% CI) compared to the MC group. Regarding the fathers, paternal Suicidal Ideation (OR 4.295, 1.747–10.599 95% CI) was the only significant factor for adolescent suicidal ideation. In contrast, maternal depressive symptoms and help-seeking behavior significantly impacted adolescent help-seeking; Maternal Depressive Mood increased with adolescent Help-Seeking (OR 4.486, 1.312–15.340 95% CI) while Maternal Suicidal Ideation reduced the probability of Help-Seeking in the SI group (OR 0.15, 0.031–0.721, 95% CI). Chronic and severe depressive symptoms in mothers could make adolescents less likely to seek help for their suicidal ideations. Therefore, clinicians working with adolescents should prioritize a family-oriented approach.
A Review of Privacy Enhancement Methods for Federated Learning in Healthcare Systems
Authors: Gu Xin , Sabrina Fariza , Fan Zongwen , Sohail Shaleeza
Published: August 2023

Federated learning (FL) provides a distributed machine learning system that enables participants to train using local data to create a shared model by eliminating the requirement of data sharing. In healthcare systems, FL allows Medical Internet of Things (MIoT) devices and electronic health records (EHRs) to be trained locally without sending patients data to the central server. This allows healthcare decisions and diagnoses based on datasets from all participants, as well as streamlining other healthcare processes. In terms of user data privacy, this technology allows collaborative training without the need of sharing the local data with the central server. However, there are privacy challenges in FL arising from the fact that the model updates are shared between the client and the server which can be used for re-generating the client’s data, breaching privacy requirements of applications in domains like healthcare. In this paper, we have conducted a review of the literature to analyse the existing privacy and security enhancement methods proposed for FL in healthcare systems. It has been identified that the research in the domain focuses on seven techniques: Differential Privacy, Homomorphic Encryption, Blockchain, Hierarchical Approaches, Peer to Peer Sharing, Intelligence on the Edge Device, and Mixed, Hybrid and Miscellaneous Approaches. The strengths, limitations, and trade-offs of each technique were discussed, and the possible future for these seven privacy enhancement techniques for healthcare FL systems was identified.
Can We Predict Prostate Cancer Metastasis Based on Biomarkers? Where Are We Now?
Authors: San Francisco Ignacio F. , Rojas Pablo A. , Bravo Juan C. , Díaz Jorge , Ebel Luis , Urrutia Sebastián , Prieto Benjamín , Cerda-Infante Javier
Published: August 2023

The incidence of prostate cancer (PC) has risen annually. PC mortality is explained by the metastatic disease (mPC). There is an intermediate scenario in which patients have non-mPC but have initiated a metastatic cascade through epithelial–mesenchymal transition. There is indeed a need for more and better tools to predict which patients will progress in the future to non-localized clinical disease or already have micrometastatic disease and, therefore, will clinically progress after primary treatment. Biomarkers for the prediction of mPC are still under development; there are few studies and not much evidence of their usefulness. This review is focused on tissue-based genomic biomarkers (TBGB) for the prediction of metastatic disease. We develop four main research questions that we attempt to answer according to the current evidence. Why is it important to predict metastatic disease? Which tests are available to predict metastatic disease? What impact should there be on clinical guidelines and clinical practice in predicting metastatic disease? What are the current prostate cancer treatments? The importance of predicting metastasis is fundamental given that, once metastasis is diagnosed, quality of life (QoL) and survival drop dramatically. There is still a need and space for more cost-effective TBGB tests that predict mPC disease.
Fallopia japonica Root Extract Ameliorates Ovalbumin-Induced Airway Inflammation in a CARAS Mouse Model by Modulating the IL-33/TSLP/NF-κB Signaling Pathway
Authors: Jin Juan , Fan Yan Jing , Nguyen Thi Van , Yu Zhen Nan , Song Chang Ho , Lee So-Yong , Shin Hee Soon , Chai Ok Hee
Published: August 2023

Fallopia japonica (Asian knotweed) is a medicinal herb traditionally used to treat inflammation, among other conditions. However, the effects of F. japonica root extract (FJE) on airway inflammation associated with combined allergic rhinitis and asthma (CARAS) and the related mechanisms have not been investigated. This study examined the effect of FJE against CARAS in an ovalbumin (OVA)-induced CARAS mouse model. Six-week-old male BALB/c mice were randomly segregated into six groups. Mice were sensitized intraperitoneally with OVA on days 1, 8, and 15, and administered saline, Dexamethasone (1.5 mg/kg), or FJE (50, 100, or 200 mg/kg) once a day for 16 days. Nasal symptoms, inflammatory cells, OVA-specific immunoglobulins, cytokine production, mast cell activation, and nasal histopathology were assessed. Administration of FJE down-regulated OVA-specific IgE and up-regulated OVA-specific IgG2a in serum. FJE reduced the production of T helper (Th) type 2 cytokines, and the Th1 cytokine levels were enhanced in nasal and bronchoalveolar lavage fluid. Moreover, FJE positively regulated allergic responses by reducing the accumulation of inflammatory cells, improving nasal and lung histopathological characteristics, and inhibiting inflammation-associated cytokines. FJE positively modulated the IL-33/TSLP/NF-B signaling pathway, which is involved in regulating inflammatory cells, immunoglobulin levels, and pro-inflammatory cytokines at the molecular level.
Analysis of the Promoter Regions of gga-miR-31 and Its Regulation by RA and C-jun in Chicken
Authors: Wang Yingjie , Kong Ruihong , Xie Ke , Hu Cai , Zhao Zongyi , Wu Yuhui , Zuo Qisheng , Li Bichun , Zhang Yani
Published: August 2023

The role of gga-miR-31 in chicken germ cell differentiation and spermatogenesis is of significant importance. The transcriptional properties of gga-miR-31 are crucial in establishing the foundation for the formation of chicken spermatogonia stem cells and spermatogenesis. In this study, a series of recombinant vectors including varying lengths of the gga-miR-31 promoter were predicted and constructed. Through the utilization of the dual luciferase reporting system, the upstream −2180~0 bp region of gga-miR-31 was identified as its promoter region. Furthermore, it was predicted and confirmed that the activity of the gga-miR-31 promoter is increased by retinoic acid (RA). The binding of RA to the gga-miR-31 and Stra8 promoter regions was found to be competitive. Through the deletion of C-jun binding sites and the manipulation of C-jun expression levels, it was determined that C-jun inhibits the activity of the gga-miR-31 promoter. Furthermore, the combined treatment of C-jun and RA demonstrated that the positive regulatory effect of RA on the gga-miR-31 promoter is attenuated in the presence of high levels of C-jun. Overall, this study establishes a foundation for further investigation into the regulatory mechanisms of gga-miR-31 action, and provides a new avenue for inducing chicken embryonic stem cells (ESC) to differentiate into spermatogonial stem cells (SSC), and sperm formation.
Utilizing Gold Nanoparticles as Prospective Radiosensitizers in 3D Radioresistant Pancreatic Co-Culture Model
Authors: Alhussan Abdulaziz , Jackson Nolan , Calisin Reinali , Morgan Jessica , Beckham Wayne , Chithrani Devika B.
Published: August 2023

Pancreatic cancer stands among the deadliest forms of cancer, and the existing treatments fall short of providing adequate efficacy. Novel and more effective treatment approaches are urgently required to address this critical medical challenge. In this study, we aimed to evaluate the anti-cancer efficacy of gold nanoparticles (GNPs) in combination with radiotherapy (RT). A 3D pancreatic cancer co-culture spheroid model of MIA PaCa-2 cancer cells and patient-derived cancer-associated fibroblasts (CAF-98) was used. The spheroids were treated with GNPs (7.5 μg/mL) and 2 Gy of RT. The spheroids’ cell viability was assessed through the CellTiter-Glo 3D assay, and an immunofluorescence assay was used to assess the DNA DSBs via the expression of the DNA damage marker 53BP1. Co-culture samples showed a 10.8% (p < 0.05) increase in proliferation and a 13.0% (p < 0.05) decrease in DNA DSB when compared to monoculture samples, However, they displayed a 175% (p < 0.001) increase in GNPs uptake when compared to monoculture spheroids. Using GNPs/RT, we were able to show a significant reduction of 6.2% (p < 0.05) in spheroid size and an increase of 14.3% (p < 0.05) in DNA DSB damage in co-culture samples. The combination of GNPs with RT demonstrated remarkable radiosensitization effects, representing a promising approach to enhance cancer treatment efficacy. These effects were particularly noteworthy in the more treatment-resistant co-culture spheroid model.
Different Tea Germplasms Distinctly Influence the Adaptability of Toxoptera aurantii (Hemiptera: Aphididae)
Authors: Lu Changhao , Shen Ni , Jiang Wenbin , Xie Bi , Zhao Runa , Zhou Guolan , Zhao Degang , He Yingqin , Chen Wenlong
Published: August 2023

Aphids are typical phloem-sucking insect pests. A good understanding regarding their feeding behavior and population dynamics are critical for evaluating host adaptation and screening of aphid-resistant resources. Herein, the adaptability of Toxoptera aurantii (Boyer) (Hemiptera: Aphididae) to different hosts was evaluated via electropenetrography and an age-stage, two-sex life table on six tea germplasms: Zikui (ZK), Zhongcha108 (ZC108), Zhongcha111 (ZC111), Qianmei419 (QM419), Meitan5 (MT5), and Fudingdabaicha (FD). Our findings revealed that the feeding activities of T. aurantii differed considerably among the host plants. T. aurantii exhibited significantly more pathway activities on ZK and FD than on the other hosts. However, the duration of feeding of T. aurantii on ZK phloem considerably decreased compared with those of the other germplasms. Life parameters indicated that T. aurantii exhibited the highest intrinsic rate of increase (r), net reproductive rate (R0), and finite rate of increase (λ) on MT5, and the maximum values of total longevity and oviposition period were recorded on FD; these variables were reduced significantly on ZK. The results of our study demonstrate that T. aurantii can successfully survive on the six tea germplasms; however, ZK was less suitable for T. aurantii and should be considered as a potential source of resistance in breeding and Integrated Pest Management.
Ethics and Meditation: A New Educational Combination to Boost Verbal Creativity and Sense of Responsibility
Authors: Hagège Hélène , Ourmi Mohammed El , Shankland Rebecca , Arboix-Calas France , Leys Christophe , Lubart Todd
Published: August 2023

Both creativity and responsibility are important higher-order skills to develop to meet the challenges of the Anthropocene, and both are related to attentional states of consciousness and to ethics. Meditation is a set of practices that trains attentional and emotional regulation. A few studies have shown that different kinds of meditation can foster different kinds of creative thinking, and others have begun to investigate the effect of the combination of meditation and ethics on ethical characteristics (but not yet on creativity or precisely on responsibility, so far). Here, we present a nonrandomized trial with an active control group among second-year science university students (n = 84) to test the effect of the secular Meditation-Based Ethics of Responsibility (MBER) program on creative potential, self-reported awareness, and sense of one’s own responsibility. The results show a large effect of the program on sense of one’s own responsibility and convergent and divergent creative writing tasks, both in conceptual–semantic and engineering-like verbal ideation. They also suggest that convergent conceptual–semantic thinking might moderate the effect of the MBER program on the awareness and sense of one’s own responsibility. This work opens up new research and educational perspectives linked to necessary behavioral changes in the Anthropocene.
Development of the IACS Unified Strength Requirements for Hatch Covers—UR S21
Authors: Lv Yining , Brubak Lars , Ishibashi Kinya , Bollero Andrea , Saltvedt Pål Arvid , Bøe Åge
Published: August 2023

International Association of Classification Societies (IACS) is responsible for many of the ship strengthrules including both generally applicable Unified Strength Requirements (UR-S) and other rules specific for various ship types. For historical reasons, there have been different structural strength requirements regarding hatch covers, hatch coamings, and related structures in IACS UR S21, S21A, and CSR (Common Structural Rules for bulk carriers and oil tankers). This paper presents the work carried out to improve the related rules and thereby the development of the unified strength requirements for hatch covers. Firstly, related IACS rules are reviewed and compared, and some major improvements to the buckling formulations are proposed for improved accuracy. Secondly, with UR S35 being developed as a unified buckling toolbox, the unified strength requirements for hatch covers—UR S21 (Rev.6, complete revision)—are developed with a standardized interface of reference to UR S35 for buckling assessment. Finally, the numerical calculations of typical stiffened panels and full hatch covers are carried out for rule verification and consequence assessment, which demonstrates that more rational hatch cover designs can be achieved based on UR S21 (rev 6).
Concurrent Convention and Non-Convention Cases: Child Abduction in England and Wales
Authors: George Rob , Netto James
Published: August 2023

The courts of England and Wales permit applicants in 1980 Hague Convention child abduction proceedings also to bring concurrent applications for the return of the child to their state of habitual residence based on a summary welfare assessment, which can be issued and heard alongside the Hague application. Given the different nature of these two applications, having them heard concurrently raises a number of challenges for the parties in terms of the evidence required and for the court in terms of the analytical process being undertaken. This article explores the nature of the two applications, the reasons why they might be brought concurrently, and the challenges that can arise in such cases.
Correction: Shi et al. Effect of Final Rolling Temperature on Microstructures and Mechanical Properties of AZ31 Alloy Sheets Prepared by Equal Channel Angular Rolling and Continuous Bending. Materials 2020, 13, 3346
Authors: Shi Laixin , Liu Lei , Hu Li , Zhou Tao , Yang Mingbo , Lian Yong , Zhang Jin
Published: August 2023

In the original publication [...]