bluuebunny/crossref_metadata_2025 huggingface dataset:
Predictors of mosquito bed net use among children under-fives in Ghana: a multilevel analysis of the 2019 malaria indicator survey
Authors: Aheto Justice Moses K. , Babah Rahmatu , Dzokoto Maxwell Kwame , Kwarah Williams , Alhassan Yakubu
Published: June 2023

Background

Morbidities and mortalities due to malaria can be prevented by the use of insecticide-treated mosquito bed nets (ITN), which has been proven for malaria control and elimination. The purpose of this study was to assess the critical factors that predict the use of ITN among children under-fives in Ghana.

Methods

The study utilized data from the 2019 Ghana Malaria Indicator Survey (GMIS). The outcome variable was mosquito bed net use among children under-fives. To determine critical factors that independently predict ITN use, multilevel multivariable logistic regression was employed using Stata version 16. Odds ratios and associated 95% confidence intervals and p-values were reported. A p < 0.05 was used to declare statistical significance.

Results

The overall prevalence of ITN usage was 57.4%. Utilization of bed nets was 66.6% in the rural areas and 43.5% in the urban areas, was highest in the Upper West region (80.6%) even when stratified to rural (82.9%) and urban areas (70.3%) whilst Greater Accra region (30.5%, rural = 41.7%, urban = 28.9%) had the least. The community level multilevel analysis showed that bed net utilization was higher among children in rural areas [AOR = 1.99, 95% CI 1.32–3.01, p = 0.001] and in household with wooden wall materials [AOR = 3.29, 95% CI 1.15–9.40, p = 0.027]. Bed net utilization was however, less for households with 3 + children under-five [AOR = 0.29, 95% CI 0.19–0.46, p < 0.001), 4 years old (AOR = 0.66, 95% CI 00.48–00.92, p = 0.014], without universal access to bed net [AOR = 0.52, 95% CI 0.37–0.73, p < 0.001], those in the Greater Accra [AOR = 0.26, 95% CI 0.13–0.51, p < 0.001], Eastern [AOR = 0.47, 95% CI 0.23–0.95, p = 0.036], Northern [AOR = 0.42, 95% CI 0.20–0.88, p = 0.022], middle [AOR = 0.57, 95% CI 0.35–0.94, p = 0.026] and rich/richest [AOR = 0.51, 95% CI 0.29–0.92, p = 0.025] household wealth quintile. Substantial unobserved household and community level differences in bed net use were found.

Conclusion

This study demonstrates the need to intensify promotion of ITN use to those in urban areas, Greater Accra, Eastern and Northern regions, houses without wooden wall materials, middle and rich/richest households. Interventions should be targeted at older children and households with more under-five children and to ensure full access and use of ITNs among all children under-fives in each household as part of the overall goal of achieving the health-related SDGs.
Identification of TIMP1 as an inflammatory biomarker associated with temporal lobe epilepsy based on integrated bioinformatics and experimental analyses
Authors: He Ya , Zhang Hongxia , Ma Limin , Li Jingang , Wang Fei , Zhou Hui , Zhang Guangliang , Wen Yuetao
Published: June 2023

Background

Epilepsy is the second most prevalent neurological disease. Although there are many antiseizure drugs, approximately 30% of cases are refractory to treatment. Temporal lobe epilepsy (TLE) is the most common epilepsy subtype, and previous studies have reported that hippocampal inflammation is an important mechanism associated with the occurrence and development of TLE. However, the inflammatory biomarkers associated with TLE are not well defined.

Methods

In our study, we merged human hippocampus datasets (GSE48350 and GSE63808) through batch correction and generally verified the diagnostic roles of inflammation-related genes (IRGs) and subtype classification according to IRGs in epilepsy through differential expression, random forest, support vector machine, nomogram, subtype classification, enrichment, protein‒protein interaction, immune cell infiltration, and immune function analyses. Finally, we detected the location and expression of inhibitor of metalloproteinase-1 (TIMP1) in epileptic patients and kainic acid-induced epileptic mice.

Results

According to the bioinformatics analysis, we identified TIMP1 as the most significant IRG associated with TLE, and we found that TIMP1 was mainly located in cortical neurons and scantly expressed in cortical gliocytes by immunofluorescence staining. We detected decreased expression of TIMP1 by quantitative real-time polymerase chain reaction and western blotting.

Conclusion

TIMP1, the most significant IRG associated with TLE, might be a novel and promising biomarker to study the mechanism of epilepsy and guide the discovery of new drugs for its treatment.
Application of platelet-rich plasma (PRP) in lips rejuvenation
Authors: Huang YuanYuan , Tang JunYi , He XiaoYing , Liu HongYun , Cheng HangHangLiu , Yang Yu , Cheng Biao , Tian Ju
Published: June 2023

Background

In recent years, minimally invasive and non-invasive rejuvenation methods have been welcomed. PRP has been used widely for skin rejuvenation, but there are few studies on PRP for lip rejuvenation.

Objective

The objective of this study was to investigate the preliminary effects of PRP for lip rejuvenation.

Methods

Between October 2018 and April 2023, 15 participants with lip aging (1 male, 14 females; range 27–58 years) were treated with PRP. The follow-up time was 3 to 24months. After 3 to 6 times treatments, beauty seekers and experienced physicians jointly evaluated effectiveness of treatment. The assessment included improvements in the colour, wrinkles, and skin texture of the lips before and after treatment.

Results

According to the beauty seekers and Surgeons ’evaluation, the aging characteristics of the lips of the 15 beauty seekers have been improved to varying degrees. The most obvious improvement was that the color of the lips which became more vivid. There was no swelling, bruising, scar hyperplasia and other complications. A participant was evaluated using the VISIA skin detector. The patient’s lip color and discoloration improved after treatment. Of the 15 participants treated. 3 participants experienced mild pain or discomfort during the injection process. There was no swelling, bruising, scar hyperplasia and other complications.

Conclusion

The results of this study revealed promising evidence of PRP as an effective option on lip rejuvenation. However, large, multi-center, controlled, long term, pilot studies are required to confirm the preliminary results of our study.
Correlation analysis of the vertebral compression degree and CT HU value in elderly patients with osteoporotic thoracolumbar fractures
Authors: Li Cheng , Lai Xing-ming , Liu Nian , Lin Yang , Hu Wei
Published: June 2023

Background

To explore the correlation of the vertebral compression degree and cancellous bone CT HU in elderly patients with osteoporotic thoracolumbar fractures.

Methods

Elderly patients with single-segment vertebral fragility fractures were retrospectively reviewed. All patients experienced a low-energy trauma and underwent thoracolumbar MRI. The consistency of measurement between two spine surgeons was evaluated. The average CT HU value of the adjacent vertebral body was used instead.

Results

A total of 54 patients were included in the final analysis. The patients’ average age was 70.39 ± 8.53 years, and the average CT HU value was 72.78 ± 29.75 HU. The average vertebral compression ratio was 0.57 ± 0.16. Measurements showed both good intrarater repeatability and good interrater reproducibility of the vertebral compression ratio (ICC = 0.978). The degree of vertebral compression in thoracolumbar osteoporotic fractures was strongly positively correlated with the cancellous bone CT HU value (P < 0.01).

Conclusions

The local bone quality as evaluated by the CT HU value is an important factor affecting the degree of compression in osteoporotic vertebral fractures. This study provides quantitative evidence that a greater compression ratio with thoracolumbar osteoporotic fractures was associated with lower bone density in elderly patients. Further longitudinal studies with larger cohorts are needed to verify this relationship.
Pituitary abscess: a case report and systematic review of 488 cases
Authors: Stringer Felicity , Foong Yi Chao , Tan Alanna , Hayman Sarah , Zajac Jeffrey D. , Grossmann Mathis , Zane Justin Ng Yau , Zhu Jasmine , Ayyappan Sujith
Published: June 2023

Background

Pituitary abscess (PA) is a rare condition and not well understood. We aimed to describe a case and perform a comprehensive systematic review to explore presenting symptoms, radiological findings, endocrine abnormalities and mortality.

Aim

To identify presenting symptoms, radiological findings, endocrinological abnormalities and predictors of mortality for PA.

Methods

We systematically reviewed the literature to identify all case reports of PA. Data regarding presentation, mortality, radiological findings, endocrinological abnormalities and treatment was extracted.

Results

We identified 488 patients from 218 articles meeting the inclusion criteria. Mortality was 5.1%, with days to presentation (OR 1.0005, 95% CI 1.0001–1.0008, p < 0.01) being the only identified independent predictor of mortality. Mortality rates have decreased over time, with cases published prior to 2000 having higher mortality rates (OR 6.92, 95% CI 2.80–17.90, p < 0.001). The most common symptom was headache (76.2%), followed by visual field defects (47.3%). Classical signs of infection were only present in 43%. The most common imaging feature on magnetic resonance imaging (MRI) was high T2 and low T1 signal of the pituitary gland with peripheral contrast enhancement. Over half (54.8%) were culture negative, with the most common bacterial organism being staphylococcus aureus (7.8%) and fungal organism being aspergillus (8.8%). The most common endocrine abnormality was hypopituitarism (41.1%), followed by diabetes insipidus (24.8%). Whilst symptoms resolved in most patients, persistent endocrine abnormalities were present in over half of patients (61.0%).

Conclusion

PA is associated with significant mortality, with delayed presentation increasing risk of mortality. Ongoing endocrinological abnormalities are common. Given the non-specific clinical presentation, the appearance of high T2, low T1 and peripheral contrast enhancement of the pituitary on MRI should prompt consideration of this rare disease.
Exosomal circRNA: emerging insights into cancer progression and clinical application potential
Authors: Zhang Fan , Jiang Jiajia , Qian Hui , Yan Yongmin , Xu Wenrong
Published: June 2023

Exosomal circRNA serves a novel genetic information molecule, facilitating communication between tumor cells and microenvironmental cells, such as immune cells, fibroblasts, and other components, thereby regulating critical aspects of cancer progression including immune escape, tumor angiogenesis, metabolism, drug resistance, proliferation and metastasis. Interestingly, microenvironment cells have new findings in influencing tumor progression and immune escape mediated by the release of exosomal circRNA. Given the intrinsic stability, abundance, and broad distribution of exosomal circRNAs, they represent excellent diagnostic and prognostic biomarkers for liquid biopsy. Moreover, artificially synthesized circRNAs may open up new possibilities for cancer therapy, potentially bolstered by nanoparticles or plant exosome delivery strategies. In this review, we summarize the functions and underlying mechanisms of tumor cell and non-tumor cell-derived exosomal circRNAs in cancer progression, with a special focus on their roles in tumor immunity and metabolism. Finally, we examine the potential application of exosomal circRNAs as diagnostic biomarkers and therapeutic targets, highlighting their promise for clinical use.
Safety and activity of the first-in-class locked nucleic acid (LNA) miR-221 selective inhibitor in refractory advanced cancer patients: a first-in-human, phase 1, open-label, dose-escalation study
Authors: Tassone Pierfrancesco , Di Martino Maria Teresa , Arbitrio Mariamena , Fiorillo Lucia , Staropoli Nicoletta , Ciliberto Domenico , Cordua Alessia , Scionti Francesca , Bertucci Bernardo , Salvino Angela , Lopreiato Mariangela , Thunarf Fredrik , Cuomo Onofrio , Zito Maria Cristina , De Fina Maria Rosanna , Brescia Amelia , Gualtieri Simona , Riillo Caterina , Manti Francesco , Caracciolo Daniele , Barbieri Vito , Di Paola Eugenio Donato , Di Francesco Adele Emanuela , Tagliaferri Pierosandro
Published: June 2023

Background

We developed a 13-mer locked nucleic acid (LNA) inhibitor of miR-221 (LNA-i-miR-221) with a full phosphorothioate (PS)-modified backbone. This agent downregulated miR-221, demonstrated anti-tumor activity against human xenografts in mice, and favorable toxicokinetics in rats and monkeys. Allometric interspecies scaling allowed us to define the first-in-class LNA-i-miR-221 safe starting dose for the clinical translation.

Methods

In this first-in-human, open-label, dose-escalation phase 1 trial, we enrolled progressive cancer patients (aged ≥ 18 years) with ECOG 0–2 into 5 cohorts. The treatment cycle was based on a 30-min IV infusion of LNA-i-miR-221 on 4 consecutive days. Three patients within the first cohort were treated with 2 cycles (8 infusions), while 14 patients were treated with a single course (4 infusions); all patients were evaluated for phase 1 primary endpoint. The study was approved by the Ethics Committee and Regulatory Authorities (EudraCT 2017-002615-33).

Results

Seventeen patients received the investigational treatment, and 16 were evaluable for response. LNA-i-miR-221 was well tolerated, with no grade 3–4 toxicity, and the MTD was not reached. We recorded stable disease (SD) in 8 (50.0%) patients and partial response (PR) in 1 (6.3%) colorectal cancer case (total SD + PR: 56.3%). Pharmacokinetics indicated non-linear drug concentration increase across the dose range. Pharmacodynamics demonstrated concentration-dependent downregulation of miR-221 and upregulation of its CDKN1B/p27 and PTEN canonical targets. Five mg/kg was defined as the recommended phase II dose.

Conclusions

The excellent safety profile, the promising bio-modulator, and the anti-tumor activity offer the rationale for further clinical investigation of LNA-i-miR-221 (ClinTrials.Gov: NCT04811898).
Characteristics of a trauma population in an ambulance organisation in Sweden: results from an observational study
Authors: Larsson Glenn , Axelsson Christer , Hagiwara Magnus Andersson , Herlitz Johan , Magnusson Carl
Published: June 2023

Background

Globally, injuries are a major health problem, and in Sweden, injuries are the second most common reason for ambulance dispatch. However, there is a knowledge gap regarding the epidemiology of injuries requiring assessment by emergency medical services (EMS) in Sweden. The aim of the present study was to describe the prehospital population with injuries that have been assessed and treated by EMS.

Methods

A randomly selected retrospective sample was collected from 1 January through 31 December 2019 in a region in southwestern Sweden. Data were collected from ambulance and hospital medical records.

Results

Among 153,724 primary assignments, 26,697 (17.4%) were caused by injuries. The study cohort consisted of 5,235 patients, of whom 50.5% were men, and the median age was 63 years. The most common cause of injury was low-energy fall (51.4%), and this was the cause in 77.8% of those aged > 63 years and in 26.7% of those aged ≤ 63 years. The injury mechanism was a motor vehicle in 8.0%, a motorcycle in 2.1% and a bicycle in 4.0%. The most common trauma location was the residential area (55.5% overall; 77.9% in the elderly and 34.0% in the younger group). In the prehospital setting, the most frequent clinical sign was a wound (33.2%), a closed fracture were seen in 18.9% and an open fracture in 1.0%. Pain was reported in 74.9% and 42.9% reported severe pain. Medication was given to 42.4% of patients before arrival in the hospital. The most frequent triage colour according to the RETTS was orange (46.7%), whereas only 4.4% were triaged red. Among all patients, 83.6% were transported to the hospital, and 27.8% received fracture treatment after hospital admission. The overall 30-day mortality rate was 3.4%.

Conclusion

Among EMS assignments in southwestern Sweden, 17% were caused by injury equally distributed between women and men. More than half of these cases were caused by low-energy falls, and the most common trauma location was a residential area. The majority of the victims had pain upon arrival of the EMS, and a large proportion appeared to have severe pain.
Dynamic expression of IGFBP3 modulate dual actions of mineralization micro-environment during tooth development via Wnt/beta-catenin signaling pathway
Authors: Zhang MengDan , Zheng Junming , Wu Siyuan , Chen Hailing , Xiang Lusai
Published: June 2023

Background

Tooth development, as one of the major mineralized tissues in the body, require fine-tuning of mineralization micro-environment. The interaction between dental epithelium and mesenchyme plays a decisive role in this process. With epithelium–mesenchyme dissociation study, we found interesting expression pattern of insulin-like growth factor binding protein 3 (IGFBP3) in response to disruption of dental epithelium–mesenchyme interaction. Its action and related mechanisms as regulator of mineralization micro-environment during tooth development are investigated.

Results

Expressions of osteogenic markers at early stage of tooth development are significantly lower than those at later stage. BMP2 treatment further confirmed a high mineralization micro-environment is disruptive at early stage, but beneficial at later stage of tooth development. In contrast, IGFBP3's expression increased gradually from E14.5, peaked at P5, and decreased afterwards, demonstrating an inverse correlation with osteogenic markers. RNA-Seq and Co-immunoprecipitation showed that IGFBP3 regulates the Wnt/beta-catenin signaling pathway activity by enhancing DKK1 expression and direct protein–protein interaction. The suppression of the mineralization microenvironment effectuated by IGFBP3 could be reversed by the DKK1 inhibitor WAY-262611, further demonstrating that IGFBP3 exerted its influence via DKK1.

Conclusion

A deeper understanding of tooth development mechanisms is essential for tooth regeneration, which have great implications for dental care. The current study demonstrated that the IGFBP3 expression is regulated in accordance with the needs of the mineralization microenvironment during tooth development, and IGFBP3 exerts its modulating action on osteogenic/odontogenic differentiation of hDPSCs by DKK1-Wnt/ beta-catenin axis.
The effects of hip- vs. knee-dominant hamstring exercise on biceps femoris morphology, strength, and sprint performance: a randomized intervention trial protocol
Authors: Crawford Scott K. , Hickey Jack , Vlisides Jessica , Chambers Jennifer S. , Mosiman Samuel J. , Heiderscheit Bryan C.
Published: June 2023

Background

The hamstrings are an important muscle group that contribute to horizontal force during sprint acceleration and are also the most injured muscle group in running-based sports. Given the significant time loss associated with hamstrings injury and impaired sprinting performance following return to sport, identifying exercises that drive adaptations that are both protective of strain injury and beneficial to sprint performance is important for the strength and conditioning professional. This paper describes the study protocol investigating the effects of a 6-week training program using either the hip-dominant Romanian deadlift (RDL) or the knee-dominant Nordic hamstring exercise (NHE) on hamstring strain injury risk factors and sprint performance.

Methods

A permuted block randomized (1:1 allocation) intervention trial will be conducted involving young, physically-active men and women. A target sample size of 32 will be recruited and enrolled participants will undergo baseline testing involving extended-field-of-view ultrasound imaging and shear wave elastography of the biceps femoris long head muscle, maximal hamstrings strength testing in both the RDL and NHE, and on-field sprint performance and biomechanics. Participants will complete the 6-week training intervention using either the RDL or NHE, according to group allocation. Baseline testing will be repeated at the end of the 6-week intervention followed by 2 weeks of detraining and a final testing session. The primary outcome will be regional changes in fascicle length with secondary outcomes including pennation angle, muscle cross sectional area, hamstring strength, and maximal sprint performance and biomechanics. An exploratory aim will determine changes in shear wave velocity.

Discussion

Despite extensive research showing the benefits of the NHE on reducing hamstring strain injury risk, alternative exercises, such as the RDL, may offer similar or potentially even greater benefits. The findings of this study will aim to inform future researchers and practitioners investigating alternatives to the NHE, such as the RDL, in terms of their effectiveness in reducing rates of hamstring strain injury in larger scale prospective intervention studies.

Trial Registration

The trial is prospectively registered on ClinicalTrials.gov (NCT05455346; July 15, 2022).
Transcutaneous measurement of renal function in two rodent models of obstructive nephropathy
Authors: Jensen Michael Schou , de Araujo Isabela Bastos Binotti Abreu , Mutsaers Henricus A.M. , Nørregaard Rikke
Published: June 2023

Objective

Glomerular filtration rate (GFR) is a key indicator of renal function. In both clinical practice and pre-clinical research, serum levels of endogenous filtration markers, such as creatinine, are often used to estimate GFR. However, these markers often do not reflect minor changes in renal function. In this study, we therefore set out to evaluate the applicability of transcutaneous GFR (tGFR) measurements to monitor the changes in renal function, as compared to plasma creatinine (pCreatinine), in two models of obstructive nephropathy, namely unilateral ureteral obstruction (UUO) or bilateral ureteral obstruction followed by release (BUO-R) in male Wistar rats.

Results

UUO animals showed a significant reduction in tGFR compared to baseline; whereas pCreatinine levels were not significantly changed. In BUO animals, tGFR drops 24 h post BUO and remains lower upon release of the obstruction until day 11. Concomitantly, pCreatinine levels were also increased 24 h after obstruction and 24 h post release, however after 4 days, pCreatinine returned to baseline levels. In conclusion, this study revealed that the tGFR method is superior at detecting minor changes in renal function as compared to pCreatinine measurements.
Successful treatment of a multifocal comminuted open fracture of humerus with severe soft tissue defect: a case report
Authors: Barati Hasan , Afzal Sina
Published: June 2023

Background

Choosing the appropriate treatment approach for a multifocal comminuted open fracture of humerus with severe soft tissue defect is a challenging issue, which could be interesting for every orthopedic surgeon especially for those working in the trauma centers.

Case presentation

This study described an innovative approach using titanium elastic nailing to treat a multifocal comminuted open fracture of humerus with severe soft tissue defect. In this study, we report a 40-year-old Persian female patient in whom the treatment achieved complete fracture union and skin graft healing by elastic medullary nailing, vacuum dressing, and skin grafting.

Conclusions

Elastic medullary nailing is a viable option for reconstruction of simultaneous comminuted fracture and soft tissue defect.
Scalable block preconditioners for saturated thermo-hydro-mechanics problems
Authors: Ordonez Ana C. , Tardieu Nicolas , Kruse Carola , Ruiz Daniel , Granet Sylvie
Published: June 2023

We are interested in the modelling of saturated thermo-hydro-mechanical (THM) problems that describe the behaviour of a soil in which a weakly compressible fluid evolves. It is used for the evaluation of the THM impact of high-level activity radioactive waste exothermicity within a deep geological disposal facility. We shall present the definition of a block preconditioner with nested Krylov solvers for the fully coupled THM equations. Numerical results reflect the good performance of the proposed preconditioners that show to be weakly scalable until more than 2000 cores and more than 1 billion degrees of freedom. Thanks to their performance and robustness, a real waste storage problem on a scale, to our knowledge, unprecedented in the field, can be addressed.
Comparison of spontaneous eruption and modified closed eruption technique with palatal traction in alignment of impacted maxillary central incisor teeth
Authors: Marek Ivo , Janková Anna , Starosta Martin , Novosad Michal , Kučera Josef
Published: June 2023

Background

Central incisor impaction is a rare condition with potentially severe clinical and psychological implications for the patient. Treatment techniques vary according to the pretreatment situation and individual factors. The aim of this study was to compare the esthetic outcomes and treatment times between two different approaches.

Materials and methods

In this retrospective study, thirty-one consecutive patients (13 boys, 18 girls; average age 9.5 ± 2.3 years) with a total of 34 impacted permanent upper central incisors were included in the study. Patients were divided into two groups according to method of treatment. Group A comprised patients in whom spontaneous eruption occurred after space opening (n = 12), and Group B comprised patients in whom teeth showed no eruption and required treatment with a modified closed eruption method with palatally oriented traction (n = 19). Treatment time and esthetic outcomes were assessed and compared between groups.

Results

The mean treatment time was 22.0 ± 6.7 months, and all teeth were successfully aligned. No statistically significant difference in average treatment time was found between groups in baseline characteristics (p > 0.05). The amount of attached gingiva was significantly smaller when compared to contralateral reference teeth in the closed eruption group (Group B; p = 0.03). However, no difference in amount of attached gingiva was found between both groups (p = 0.26). Additionally, no difference in the clinical crown length was found between groups (p = 0.27).

Conclusion

The closed eruption method with palatal traction directed at the peak of the alveolar crest provided results comparable to the physiologic tooth eruption.
Pre-activated biochar by fertilizers mitigates nutrient leaching and stimulates soil microbial activity
Authors: Brtnicky Martin , Mustafa Adnan , Hammerschmiedt Tereza , Kintl Antonin , Trakal Lukas , Beesley Luke , Ryant Pavel , Omara-Ojungu Carol , Baltazar Tivadar , Holatko Jiri
Published: June 2023

Background

Previous studies have suggested that the targeted application of biochars in agricultural soils may benefit soil health and crop production. Physico-chemical properties of soils after biochar addition have been explored, but less is known about how microbial parameters respond. Therefore, impact of biochar (NB), mineral fertilizer-activated biochar (AB), or mineral fertilizer (MF) application on selected chemical and microbial parameters of lettuce-planted soil was evaluated in a pot experiment.

Results

In comparison to the control, soil enzymes activities, related to carbon (C), nitrogen (N), and phosphorus (P) cycling, and their content in plant biomass, were significantly increased by the addition of mineral fertilizer with or without biochar (MF, NB + MF). Conversely, microbial respiration (basal and substrate induced) was highly responsive to the activated biochar amendment (AB) as compared to other treatments. N, P, and potassium (K) concentrations in soil pore water were stabilized by the mineral fertilizer-activated biochar, indicating reduced leaching and the likelihood of increased longevity of these nutrients in soils. Enhanced carbon acquisition and mitigated nitrogen acquisition in soil of the most experimental treatments were coupled with higher crop (lettuce) biomass.

Conclusions

Our study demonstrates that the application of biochar both with and without mineral fertilizer has the potential to enhance microbial activity and fertility in the tested agricultural soil, but that leaching of fertilizer-borne nutrients may be mitigated by the activation process.

Graphical Abstract
An epidemiological study of unintentional pediatric firearm fatalities in the USA, 2009–2018
Authors: Vaishnav Arti , Smith Gary A. , Badeti Jaahnavi , Michaels Nichole L.
Published: June 2023

Background

Firearm injuries are the leading cause of mortality among children and adolescents 1–19 years old in the USA. Many prior studies on this topic lack detailed information about the circumstances of the firearm fatalities and include decedents and shooters of all ages. This study characterizes firearm fatalities in the USA in which children < 15 years old unintentionally killed themselves or another child.

Methods

Ten years of data from the National Violent Death Reporting System were analyzed. Unintentional firearm fatalities among children were reviewed to identify characteristics of decedents and the children who inflicted the deaths, their relationship, and circumstances of the deaths. There were 279 firearm fatalities during the study period involving children < 15 years old who unintentionally killed themselves or another child < 15 years old.

Results

Most victims were male (81.4%), and 40.9% were 2–4 years old. Most incidents (64.0%) occurred at the victim’s residence, and in 80.9% of cases the firearm owner was a relative of the shooter. In the < 5-year age group, 80.3% of injuries were self-inflicted, and in the 10–14-year age group, 32.3% of shooters were a friend of the victim.

Conclusion

This study highlights that children in the USA are shooting themselves and each other in their own homes, and often accessing firearms owned by family members. These findings can be used to guide prevention efforts, such as child access prevention laws, to reduce the number of pediatric firearm fatalities in the future.
The efficiency of the electro-osmosis method on the consolidation and strength properties of the gray clay of Tabriz
Authors: Zareh Ali , Poursorkhabi Ramin Vafaei , Majdi Alireza Alizadeh , Sarand Fariba Behrouz
Published: June 2023

Background

Consolidation of clays could be a very time-consuming procedure. However, preloading can increase the speed of consolidation; applying an electric field can accelerate this procedure significantly. The efficiency of the electroosmosis approach has been studied for different types of clays previously. At the same time, there is no information about the performance of the EO method for Tabriz gray clay.

Methods

This paper investigates the effects of the EO technology on Tabriz gray clay using a modified oedometer consolidation apparatus. The achieved results of the EO consolidation of the Gray clay of Tabriz are compared with the standard Kaolin clay (KC-which produced industrially). Three different electrodes (Iron, Copper, and Aluminum) are employed to study about effects of the electrode type, and two different voltages (12 V and 24 V) are applied to assess the impact of the input electric field on the settlement of samples.

Results

It is shown that the copper electrode leads to maximum volumetric strains while the iron electrode results in minimum volumetric strains. In addition, it is shown that the larger input voltage leads to larger settlements. The results indicate that the EO approach is more efficient for the Gray clay of Tabriz in comparison to the KC, where gray clay shows larger settlements while subjected to an electric field. Unconfined compression tests are also applied to some samples, and the EO method’s influence on the samples’ strength is discussed.

Conclusions

The achieved results demonstrated that the EO method can be efficiently used to improve the consolidation and strength properties of the gray clay of Tabriz.
Combined treatment with surgery and immune checkpoint inhibitor extended survival in a case of gastric intramural metastasis from esophageal cancer: a case report
Authors: Wakasugi Ayako , Kimura Yasue , Natsugoe Keita , Nakanoko Tomonori , Nonaka Kentaro , Nambara Sho , Hu Qingjiang , Nakanishi Ryota , Ota Mitsuhiko , Oki Eiji , Oda Yoshinao , Yoshizumi Tomoharu
Published: June 2023

Background

Intramural metastasis (IM) of esophageal cancer is classified as distant metastasis according to the Japanese Classification of Esophageal Cancer, and it is well-known to be associated with a poor prognosis. We herein report a case of perforated gastric IM of esophageal cancer that was successfully controlled with nonradical surgery and subsequent immune checkpoint inhibitor (ICI) treatment.

Case presentation

A 72-year-old woman was referred to our department for the treatment of esophageal cancer and perforated gastric ulcer. A histological examination of the main tumor and gastric ulcer lesion revealed squamous cell carcinoma. Since the gastric wall tumor had invaded the celiac artery, complete resection was considered impossible. Chemotherapy was administered but led to severe adverse events, so palliative resection was performed. Two months after surgery, computed tomography revealed enlargement of the residual tumor around the celiac artery. However, after nivolumab monotherapy was started, the tumor diminished remarkably, and the quality of life of the patient dramatically improved. Nine months after nonradical surgery, she is surviving without any disease concern.

Conclusions

With the increased availability of ICIs, multidisciplinary treatment with surgery and ICIs can potentially lead to long-term survival, even in cases expected to have a poor prognosis.
Evaluation of dietary food intakes and anthropometric measures in middle-aged men with aggressive symptoms
Authors: Abiri Behnaz , Amini Shirin , Ehsani Hajar , Ehsani MohammadAli , Adineh Parisa , Mohammadzadeh Hakimeh , Hashemi Sima
Published: June 2023

Background

Aggression is one of the most prevalent behavioral disorders in men.

Objective

This study aimed to assess the possible association between dietary intake of food groups and aggression in middle-aged married men.

Methods

This case-control study included 336 participants (168 men with aggressive behaviors and 168 healthy controls) aged 35–55 years. Demographic information was collected using a socio-demographic questionnaire. A food frequency questionnaire was used to investigate the diet group intake last year. Based on the normality of the data distribution, Independent t-tests and Mann-Whitney tests were used to compare quantitative variables between the two groups. Categorical variables were compared between cases and controls using the Chi-squared test. Logistic regression analysis was used to examine the possible association between food intake and aggression.

Results

Compared to controls, aggressive men had significantly higher mean weight, height, and waist circumference (WC), p = 0.007, p = 0.001, and p = 0.043, respectively. After adjusting WC, energy intake, and educational level, in Model 1, intake of milk, cheese, poultry, red meat, legumes, egg, fruits, and vegetables had a significant protective role on the occurrence of aggression, (Odd Ratio (OR) = 0.36; 95% (Confidence Interval (CI) = 0.204, 0.670; P = 0.001), (OR = 0.440; 95% CI = 0.284, 0.781; P = 0.005), (OR = 0.621; 95% CI = 0.284, 0.781; P = 0.046), (OR = 0.358; 95% CI = 0.198, 0.647; P = 0.001), (OR = 0.434; 95% CI = 0.243, 0.773; P = 0.005), (OR = 0.411; 95% CI = 0.229, 0.736; P = 0.003), (OR = 0.332; 95% CI = 0.180, 0.614; P < 0.001), (OR = 0.310; 95% CI = 0.168, 0.572; P < 0.001), respectively.

Conclusions

Lower WC and a diet containing high-quality protein, fruits, and vegetables can have a protective role against aggression and are recommended for men with an aggressive mood. This diet can affect plasma levels of tryptophan and, therefore, brain levels of serotonin.
Development and validation of a questionnaire to examine determinants of consumer intentions to purchase organic food
Authors: Bazhan Marjan , Shafiei Sabet Farnam , Borumandnia Nasrin
Published: June 2023

Background

Organic farming is a relatively new concept in developing countries compared to developed countries. Understanding the factors affecting consumers’ willingness to pay for organic foods is critical to increasing the production of these products. This study aimed to develop and validate a Persian version of the questionnaire for assessing determinants of organic food purchase intention among adults in Tehran, the capital of Iran.

Methods

The study was conducted in a two-phased standardized methodology in 2019. During Phase 1, a draft questionnaire was developed based on a comprehensive literature review. In phase 2, validation of the instrument was performed. Participants included a multidisciplinary expert panel comprising 14 members to evaluate content validity, a sample of lay people to assess face validity (n = 20), internal consistency (n = 300), and test-retest reliability (n = 62). The internal consistency and test-retest reliability were measured using the intraclass correlation coefficient (ICC) and Cronbach’s alpha.

Results

Forty-nine of 57 items had a CVR above 0.51 and were retained in the questionnaire. Three items were added to the questionnaire. The average CVI for the questionnaire was 0.97. Cronbach’s α and ICC of the entire questionnaire were 0.86 and 0.93, respectively. Each phase of development progressively improved the questionnaire, resulting in a final 52-item questionnaire divided into 9 dimensions, including knowledge, attitude, subjective norms, health consciousness, environmental concerns, perceived convenience of purchase, perceived cost, sensory characteristics, and purchase intention.

Conclusions

The developed questionnaire appears to be a valid and reliable instrument for examining determinants of consumer intentions to purchase organic food.
Informal childcare arrangements: a comparison between Italians and migrants
Authors: Trappolini Eleonora , Terzera Laura , Rimoldi Stefania M. L. , Barbiano di Belgiojoso Elisa
Published: June 2023

As migrants settle in their destination country, for those who reunited the family or after childbirth childcare becomes a priority. Most studies on migrants’ childcare arrangements have focused on parental use of formal childcare rather than on different informal childcare solutions by analysing only families with preschool-age children. Italy poses an interesting case study because its welfare system is characterised by a familistic model of care, based on solidarity between generations. In familistic countries, migrants’ childcare solutions are more constrained. In this study, we analysed differences in informal childcare needs and arrangements for children younger than 14 between Italians and migrants from different countries of origin. We merged two surveys conducted by the Italian National Statistics Institute in 2011–2012: ‘Social Condition and Integration of Foreign Citizens’, a sample of households with at least one migrant with foreign citizenship, and ‘Multiscopo—Aspects of Daily Life’, a sample of households in Italy. We found that household composition and parents’ employment status play an important role in shaping informal childcare arrangements. Overall, migrants are less likely to use informal childcare, especially grandparents, than Italians but when they do, they rely more on other relatives and non-relatives than Italians. Moreover, differences emerge across migrant subgroups. This study is the first in Italy to contribute to an understanding of the role of migrant status in determining parents’ childcare arrangements for children up to 13 years.
The reliability and validity of the TBI-CareQOL system in four diverse caregiver groups
Authors: Carlozzi Noelle E. , Choi Sung Won , Wu Zhenke , Sen Srijan , Troost Jonathan , Lyden Angela K. , Miner Jennifer A. , Graves Christopher , Sander Angelle M.
Published: June 2023

Purpose

Establishing the psychometric reliability and validity of new measures is an ongoing process. More work is needed in to confirm the clinical utility of the TBI-CareQOL measurement development system in both an independent cohort of caregivers of traumatic brain injury (TBI), as well as in additional caregiver groups.

Methods

An independent cohort of caregivers of people with TBI (n = 139), as well as three new diverse caregiver cohorts (n = 19 caregivers of persons with spinal cord injury, n = 21 caregivers for persons with Huntington disease, and n = 30 caregivers for persons with cancer), completed 11 TBI-CareQOL measures (caregiver strain; caregiver-specific anxiety; anxiety; depression; anger; self-efficacy; positive affect and well-being; perceived stress; satisfaction with social roles and activities; fatigue; sleep-related impairment), as well as two additional measures to examine convergent and discriminant validity (PROMIS Global Health; the Caregiver Appraisal Scale).

Results

Findings support the internal consistency reliability (all alphas > 0.70 with the vast majority being > 0.80 across the different cohorts) of the TBI-CareQOL measures. All measures were free of ceiling effects, and the vast majority were also free of floor effects. Convergent validity was supported by moderate to high correlations between the TBI-CareQOL and related measures, while discriminant validity was supported by low correlations between the TBI-CareQOL measures and unrelated constructs.

Conclusion

Findings indicate that the TBI-CareQOL measures have clinical utility in caregivers of people with TBI, as well as in other caregiver groups. As such, these measures should be considered as important outcome measures for clinical trials aiming to improve caregiver outcomes.
Falls risk perception measures in hospital: a COSMIN systematic review
Authors: Dabkowski Elissa , Missen Karen , Duncan Jhodie , Cooper Simon
Published: June 2023

Falls prevention in hospital continues to be a research priority because of the poor health outcomes and financial burdens that can arise. Recently updated World Guidelines for Falls Prevention and Management strongly recommend evaluating patients’ concerns about falling as part of a multifactorial assessment. The aim of this systematic review was to evaluate the quality of falls risk perception measures for adults in a hospital setting. This review was conducted using the Consensus-based Standards for the selection of health Measurement Instruments guidelines and provides a comprehensive summary of these instruments, including psychometric properties, feasibility and clinical recommendations for their use. The review followed a prospectively registered protocol, in which a total of ten databases were searched between the years 2002 and 2022. Studies were included if the instruments measured falls risk perception and/or other psychological falls constructs, if they were conducted in a hospital setting and if the target population contained hospital inpatients. A total of 18 studies met the inclusion criteria, encompassing 20 falls risk perception measures. These falls risk perception instruments were grouped into five falls-related constructs: Balance Confidence, Falls Efficacy/Concern, Fear of Falling, Self-Awareness and Behaviour/Intention. Two of the patient reported outcome measures (PROMs) received Class A recommendations (Falls Risk Perception Questionnaire and the Spinal Cord Injury-Falls Concern Scale); however, this rating is only applicable for the populations/context described in the studies. Thirteen PROMs received Class B recommendations, solidifying the need for further validation studies of these PROMs.
Effect of titanium oxide nanoparticles on polymerization reaction of heat and microwave cured polymethylmethacrylate: in vitro study
Authors: Ibraheem Eman M. , Elboraey Asmaa N. , Moussa Amani R. , Khalil Safaa K. H. , Dehis Wessam M.
Published: June 2023

Background

Titanium Oxide Nanoparticles (TiO2NPs) have been introduced to polymethyl methacrylate (PMMA) denture base to enhance its mechanical, physical, and biological performance.

Objectives

This research aimed to assess the effect of adding TiO2NPs on the degree of polymerization and morphological characterization of heat and microwave cured PMMA.

Materials and methods

Fabrication of PMMA specimens which were categorized according to the curing technique into heat cured (group I) and microwave cured (group II). Each group was subdivided into two subgroups A and B according to the addition of TiO2NPs. A total number of 24 specimens were fabricated. Each subgroup contains 6 specimens. The specimens were characterized by Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray analysis (EDX). The polymerization reaction of specimens was assessed by Attenuated Total Reflection Fourier-Transform Infra-Red Spectroscopy (ATR-FTIR). Results were statistically analyzed using an independent T-test and one-way ANOVA. The significance level was set at p < 0.05.

Results

SEM/EDX revealed that TiO2 NPs appeared as bright areas in the matrix of both groups. The matrix showed organic impurities and minute internal cracks, but group IIB (microwave-cured PMMA with TiO2NPs) showed higher organic impurities than heat-cured PMMA. The FTIR revealed that the addition of 3% by weight TiO2NPs decreased the degree of polymerization of both heat and microwave-cured PMMA but the decrease was insignificant (p > 0.05).

Conclusions

Incorporation of 3% by weight TiO2NPs does not significantly affect the degree of polymerization of both heat and microwave-cured PMMA. Hence, denture materials can benefit from the advantages of the TiO2NPs without any alterations to their structures.

Graphical abstract
Naked-eye observation of water-forming reaction on palladium etalon: transduction of gas-matter reaction into light-matter interaction
Authors: Lee Jongsu , Yu Eui-Sang , Kim Taehyun , Kim In Soo , Chung Seok , Kwak Seung Jae , Lee Won Bo , Pak Yusin , Ryu Yong-Sang
Published: June 2023

Palladium is the most prominent material in both scientific and industrial research on gas storage, purification, detection, and catalysis due to its unique properties as a catalyst and hydrogen absorber. Advancing the dynamic optical phenomena of palladium reacting with hydrogen, transduction of the gas-matter reaction into light-matter interaction is attempted to visualize the dynamic surface chemistry and reaction behaviors. The simple geometry of the metal-dielectric-metal structure, Fabry–Perot etalon, is employed for a colorimetric reactor, to display the catalytic reaction of the exposed gas via water-film/bubble formation at the dielectric/palladium interface. The adsorption/desorption behavior and catalytic reaction of hydrogen and oxygen on the palladium surface display highly repeatable and dramatic color changes based on two distinct water formation trends: the foggy effect by water bubbles and the whiteout effect by water film formation. Simulations and experiments demonstrate the robustness of the proposed Fabry–Perot etalon as an excellent platform for monitoring the opto-physical phenomena driven by heterogeneous catalysis.
A rare cause of recurrent lower gastrointestinal hemorrhage due to a rectosigmoid Dieulafoy’s lesion
Authors: Wifi Mohamed-Naguib , Abdellatef Abeer , Lasheen Amr Maged
Published: June 2023

Background

Colonic Dieulafoy’s lesions (DLs) are an extremely rare cause of acute gastrointestinal bleeding. However, it could be presented from self-limiting, mild bleeding episodes up to recurrent, massive life-threatening hemorrhage.

Case presentation

We present a case of 9-year-old female with recurrent attacks of hematochezia. The colonoscopy showed actively bleeding Dieulafoy’s lesions at the rectosigmoid colon that was successfully managed endoscopically by the application of two hemoclips.

Conclusion

A prompt and accurate diagnosis in patients with a Dieulafoy’s lesion carries paramount importance. A meticulous examination and proper resuscitation are a must because massive, uncontrolled bleeding from this lesion may culminate in the death of the patient fairly quickly.