bluuebunny/crossref_metadata_2025 huggingface dataset:
Perspectives and Misconceptions of an Online Adult Male Cohort Regarding Prostate Cancer Screening
Authors: Sheetz Tyler , Posid Tasha , Khuhro Aliza , Scimeca Alicia , Beebe Sarah , Gul Essa , Dason Shawn
Published: October 2024

Introduction: Congruent with most guideline publishers, the Canadian Urological Association (CUA) recommends shared decision-making (SDM) on PSA screening (PSAS) for prostate cancer (PCa) following a discussion of its benefits and harms. However, there are limited data on how the general male population feels about these topics. Methods: A survey was completed by 906 male-identifying participants (age > 18) recruited via Amazon Mechanical Turk (MTurk), which is a crowdsourcing platform providing minimal compensation. Participants answered questions regarding demographics (15), personal/family history (9), PCa/PSA knowledge (41), and opinions regarding PSAS (45). Results: The median age was 38.2 (SD = 12.0), with 22% reporting a family history of PCa and 20% reporting personally undergoing PSAS. Although most participants had heard of PCa (85%) and that they could be screened for it (81%), they generally did not feel knowledgeable about PCa or PSAS guidelines. Most want to talk to their clinician about PCa and PSAS (74%) and are supportive of SDM (48%) or patient-centered decision-making (25%). In general, participants thought PSAS was still worthwhile, even if it led to additional testing or side effects. Similarly, participants thought higher-risk patients should be screened earlier (p < 0.001). A number of misconceptions were evident in the responses. Conclusions: Men approaching the age of PSAS do not feel knowledgeable about PCa or PSAS and want their clinician to discuss these topics with them. The majority believe in PSAS and would like to undergo this screening following SDM. Clinicians also have a role in correcting common misconceptions about PCa.
Clinical Insights and Future Prospects: A Comprehensive Narrative Review on Immunomodulation Induced by Electrochemotherapy
Authors: Ferioli Martina , Perrone Anna Myriam , De Iaco Pierandrea , Zamfir Arina A. , Ravegnini Gloria , Buwenge Milly , Fionda Bruno , Galietta Erika , Donati Costanza M. , Tagliaferri Luca , Morganti Alessio G.
Published: October 2024

Electrochemotherapy (ECT) is an emerging therapeutic approach gaining growing interest for its potential immunomodulatory effects in cancer treatment. This narrative review systematically examines the current state of knowledge regarding the interplay between ECT and the immune system. Through an analysis of preclinical and clinical studies, the review highlights ECT capacity to induce immunogenic cell death, activate dendritic cells, release tumor antigens, trigger inflammatory responses, and occasionally manifest systemic effects—the abscopal phenomenon. These mechanisms collectively suggest the ECT potential to influence both local tumor control and immune responses. While implications for clinical practice appear promising, warranting the consideration of ECT as a complementary treatment to immunotherapy, the evidence remains preliminary. Consequently, further research is needed to elucidate the underlying mechanisms, optimize treatment protocols, explore potential synergies, and decipher the parameters influencing the abscopal effect. As the field advances, the integration of ECT’s potential immunomodulatory aspects into clinical practice will need careful evaluation and collaboration among clinical practitioners, researchers, and policymakers.
Clinical Outcome Patterns of Use of Radium-223 in Patients with Metastatic Castration-Resistant Prostate Cancer
Authors: Mackenzie Colleen , Deluce Jasna , Black Morgan , Churchman Emma , Winquist Eric , Ernst Scott , Laidley David T. , Parezanovic Matthew , Potvin Kylea , Fernandes Ricardo
Published: October 2024

Introduction: Radium-223 dichloride (radium-223) is a bone-targeting radioisotope therapy that aids in the survival of patients with metastatic castration-resistant prostate cancer (mCRPC) to bones. This study aimed to describe the clinical characteristics and outcomes of patients with mCRPC treated with radium-223 in a real-world setting. Methods: This was a retrospective study of patients with mCRPC treated with radium-223 between 2016 and 2020 at the London Health Sciences Centre in London, Canada. The baseline characteristics between the patients receiving 1–3 and 4–6 treatment cycles were compared using a two-sample t-test and Chi-square test. ANOVA was used to determine if there was a difference in each diagnostic variable per treatment cycle. Kaplan–Meier curves were generated to estimate progression-free survival (PFS) and overall survival in the patients treated with different numbers of cycles. Results: Fifty eligible patients were identified. The median age was 71 years (IQR: 66–76). Most patients (62%) received radium-223 beyond the third-line treatment. The mean number of radium-223 treatments was four. While 60% of the patients received 4–6 injections, 40% received 1–3 injections. Fifty-eight percent (58%) of the patients demonstrated a clinical benefit, with the remainder expressing either disease progression (28%) or stable disease (10%). The patients treated with 4–6 cycles had a delay to disease progression compared to those given 1–3 cycles of radium-223 (F5,35 = 10.52, p < 0.001). A higher alkaline phosphatase level prior to treatment was associated with a longer PFS (z33 = 2.362, p = 0.018). Treatment-related hospitalization for skeletal-related events was noted in 8% of the patients, and 14% required treatment discontinuation due to hematologic toxicity. Conclusions: This study confirms the safety of radium-223 in patients with mCRPC in a real-world setting. The radium-223 treatment was associated with a clinical benefit in the majority of the patients, particularly in those with higher pre-treatment serum alkaline phosphatase levels. Further studies to identify the predictive biomarkers are warranted to better guide the contemporary use of radium-223.
Impact of Hypoglycemia on Glucose Variability over Time for Individuals with Open-Source Automated Insulin Delivery Systems
Authors: Shahid Arsalan , Lewis Dana M.
Published: October 2024

This study investigates glucose conditions preceding and following various hypoglycemia levels in individuals with type 1 diabetes using open-source automated insulin delivery (AID) systems. It also seeks to evaluate relationships between hypoglycemia and subsequent glycemic variability. Methods: Analysis of continuous glucose monitor (CGM) data from 122 adults with type 1 diabetes using open-source AID from the OpenAPS Data Commons was conducted. This study comprehensively analyzed the effects of hypoglycemia on glycemic variability, covering time periods before and after hypoglycemia. Results: Glucose variability normalization post-hypoglycemia can take up to 48 h, with severe hypoglycemia (41–50 mg/dL) linked to prolonged normalization. A cyclical pattern was observed where hypoglycemia predisposes individuals to further hypoglycemia, even with AID system use. A rise in glucose levels often precedes hypoglycemia, followed by an elevated mean time above range (TAR) post-hypoglycemia, indicating a ‘rebound’ effect. The experimental results are further validated on T1DEXI data (n = 222), originating from commercial AID systems. Different hypoglycemia categorization approaches did not show significant differences in glycemic variability outcomes. The level of hypoglycemia does influence the pattern of subsequent glucose fluctuations. Conclusion: Hypoglycemia, especially at lower levels, significantly impacts subsequent glycemic variability, even with the use of open-source AID systems. This should be studied further with a broader set of commercial AID systems to understand if these patterns are true of all types of AID systems. If these patterns occur in all types of AID systems, it underscores potential opportunities for enhancements in AID algorithms and highlights the importance of educating healthcare providers and people with diabetes about post-hypoglycemia glucose variability.
Adenomyosis and Adolescence: A Challenging Diagnosis and Complex Management
Authors: Martire Francesco Giuseppe , d’Abate Claudia , Schettini Giorgia , Cimino Giulia , Ginetti Alessandro , Colombi Irene , Cannoni Alberto , Centini Gabriele , Zupi Errico , Lazzeri Lucia
Published: October 2024

Adenomyosis is a chronic, hormone-related disease characterized by the presence of the endometrial glands and stroma within the myometrium. This condition can manifest in various features, focal or diffuse adenomyosis or as an adenomyoma, and it may involve different uterine walls (posterior, anterior, and/or lateral walls). The disease can also be classified into different degrees, as mild, moderate and severe, which can be associated with more intense symptoms, although this correlation is not always directly proportional. In fact, adenomyosis can be asymptomatic in about a third of cases or it can significantly impact patients’ quality of life through painful symptoms, such as dysmenorrhea and dyspareunia, abnormal uterine bleeding—particularly heavy menstrual bleeding—and potential effects on fertility. Historically, adenomyosis has been considered a disease primarily affecting premenopausal women over the age of 40, often multiparous, because the diagnosis was traditionally based on surgical reports from hysterectomies performed after the completion of reproductive desire. Data on the presence of adenomyosis in adolescent patients remain limited. However, in recent years, advancements in noninvasive diagnostic tools and increased awareness of this pathology have enabled earlier diagnoses. The disease appears to have an early onset during adolescence, with a tendency to progress in terms of extent and severity over time. Adenomyosis often coexists with endometriosis, which also has an early onset. Therefore, it is important, when diagnosing adenomyosis, to also screen for concomitant endometriosis, especially deep endometriosis in the posterior compartment. The aim of this narrative review is to investigate the prevalence of different types and degrees of adenomyosis in younger patients, assess the associated symptoms, and describe the most appropriate diagnostic procedures for effective therapeutic management and follow-up, with the goal of improving the quality of life for these young women.
Primary Large-Cell Neuroendocrine Carcinoma of the Breast
Authors: Zhang Bicheng , Huang Fengbo , Guo Siyu , Wu Dang , Xiao Xiaofang , Zhang Ting
Published: October 2024

Breast neuroendocrine carcinoma (NECB) is a rare type of breast tumor. Large-cell neuroendocrine carcinomas of the breast (LCNECB) are a special and rare histological subtype of NECB. Here, we present a case of a 59-year-old woman who was diagnosed with an LCNECB. A mass in the upper outer quadrant of the right breast was revealed via imaging. A histological examination showed the tumor cells were composed of clusters of large cells with obvious atypia that were polygonal or irregularly shaped. The patient underwent a right-breast-conserving radical surgery and sentinel lymph node biopsy (SLB). A histopathological examination revealed that the tumor of the right breast was 2.5 × 2 cm in size with vascular invasion, and the sentinel lymph node was negative. The immunohistochemical results showed that the tumor cells were diffuse and positive for chromogranin A (CgA), synaptophysin (Syn), and INSM1. The patient successfully completed chemotherapy and radiotherapy and is currently undergoing endocrine therapy.
Predictive Factors of Significant Findings on Capsule Endoscopy in Patients with Suspected Small Bowel Bleeding
Authors: Alali Ali A. , Alrashidi Reem , Allahow Farah , Dangi Abhijit , Alfadhli Ahmad
Published: October 2024

Background: Small bowel capsule endoscopy (SBCE) is an established non-invasive diagnostic modality for a variety of small bowel pathologies and has a significant role in altering the treatment course. The diagnostic yield of SBCE in the published literature varies widely between 45 and 75%. Furthermore, it is unclear if any patient-related factors predict higher diagnostic yield. The aim of this study is to report the diagnostic yield of SBCE for suspected small bowel disease and identify any predictive factors for identifying significant pathology on SBCE. Method: A retrospective study was conducted at Mubarak Al-Kabeer Hospital in Kuwait for patients who underwent SBCE between October 2013 and February 2022. All patients underwent upper and lower endoscopy prior to referral for SBCE. Patients’ medical records were reviewed to determine SBCE indications, results, and complications. The significance of the SBCE finding was classified according to the Saurin system. A logistic regression was performed to characterize baseline predictors for identifying significant pathology on SBCE. Results: Overall, 210 patients underwent SBCE and were included in the analysis. The mean age was 57.9 years (SD 18.5), and 129 (61.4%) were males. The most common indication for SBCE was obscure occult gastrointestinal bleed (75.7%), obscure overt gastrointestinal bleed (28.6%), and investigating gastrointestinal symptoms (7.6%). Adequate bowel preparation was achieved in most patients (88.1%), imaging of the entire small bowel was achieved in 194 patients (92.4%), and no adverse events were recorded. The overall diagnostic yield of SBCE for small bowel disease was 68.1%. The most common findings were vascular lesions in the small bowel (40.0%), small bowel ulcers (22.9%), and erosions (22.9%). On multivariate regression analysis, melena at baseline was significantly associated with increased odds of identifying high-risk lesions (Saurin class P2) (OR 2.1, 95%CI 1.03–4.30, p = 0.04). Conclusions: SBCE is an effective and safe tool for investigating small bowel pathology with a diagnostic yield of 68.1% in carefully selected patients undergoing such a test. Melena at baseline is the strongest predictor of identifying high-risk lesions, and patients with which should be prioritized for SBCE.
Diagnostic Performance of Red Blood Cell Indices in the Differential Diagnosis of Iron Deficiency Anemia and the Thalassemia Trait in Chile: A Retrospective Study
Authors: Balcázar-Villarroel Mario , Mancilla-Uribe Angélica , Navia-León Sandra , Carmine Florencia , Birditt Katherine , Sandoval Cristian
Published: October 2024

Background: Iron deficiency anemia (IDA) and the β-thalassemia trait (BTT) are two main causes of hypochromic–microcytic anemia worldwide. Researchers have described many red blood cell (RBC) indices as screening tests for presumptive differentiation, based on differences observed in complete blood count (CBC) data for each condition. There are few BTT reports in Chile, and neither laboratories nor clinical staff have widely used these indices. Objective: The objective of this study was to evaluate the diagnostic performance of 29 RBC indices in 182 patients (51 BTT and 131 IDA) and compare CBC results in both groups. Methods: A retrospective search was carried out in the Laboratory Information System between January 2021 and February 2024 to collect results from CBC, and 29 RBC indices were calculated for each patient. Then, sensitivity, specificity, positive predictive value, negative predictive value, Youden’s index, positive likelihood ratio, negative likelihood ratio, and diagnostic accuracy were calculated using MedCalc©. Results: The Green and King, Wongprachum, and Keikhaei indices showed the best discriminatory power with Youden index values of 0.923, 0.908, and 0.896, respectively, and significant differences were observed in all CBC parameters between BTT and IDA patients (p < 0.001). Conclusions: The Green and King, Wongprachum, and Keikhaei indices showed the best performance; therefore, they can be used as screening for the differential diagnosis between BTT and IDA in order to improve diagnosis given the important therapeutic and epidemiological implications. In this way, clinical laboratories could have a main role in the investigation of these patients.
Mid-Infrared Emission in Ge/Ge1-xSnx/Ge Quantum Well Modeled Within 14-Band k.p Model
Authors: Zitouni Omar , Mastour Nouha , Ridene Said
Published: October 2024

Band structure and gain in a Ge/Ge1-xSnx/Ge quantum well are described theoretically using a 14-band k.p model. It has been shown that the quantum well width and the α-Sn concentration considerably modify the conduction and valence subband structure, and, as a result, the optical gain changes with the insertion of a very small concentration of α-Sn. In particular, we have determined the necessary injection carrier density Nj and the critical α-Sn concentration for elevated high gain lasing. It is found that for Nj = 1.5 × 1018 cm−3, we achieved a maximum peak gain for α-Sn concentration of the order 0.155. We can predict that Ge/Ge1-xSnx/Ge QWs should be manufactured with an α-Sn concentration less than 0.155 in devices for optoelectronics applications such as telecommunication and light emitting laser diodes.
Coordinated Planning for Multiarea Wind-Solar-Energy Storage Systems That Considers Multiple Uncertainties
Authors: Wu Weijie , Li Yixin , Wang Shu , Wang Zheng , Zhou Shucan , Zhang Yining , Zheng Minjia
Published: October 2024

As the scale of renewable energy sources (RESs) expands, it is essential to optimize the configuration of wind, solar, and storage resources across different areas. Nevertheless, the unavoidable uncertainties associated with both energy supply and demand present significant challenges for planners. This study aims to address the challenge of coordinated planning for multiarea wind-solar-energy storage systems considering multiple uncertainties. First, uncertainties related to future peak demand, thermal generation output boundaries, demand variability, and stochastic unit production are analyzed and modeled on the basis of robust optimization and stochastic programming techniques. Then, a hierarchical coordinated planning model that incorporates both system-wide (SW) and local area (LA) planning models is proposed. The SW planning model is designed to manage the optimal capacity configuration of RESs and energy storage systems (ESSs) within each LA, as well as the operational boundary of LAs. The LA planning models aim to further optimize the capacities of RESs and ESSs and minimize the economic cost within each LA on the basis of local resource characteristics. To achieve the optimal solution, the analytical target cascading (ATC) algorithm is integrated with the column-and-constraint generation (C&CG) algorithm. The simulation results validate the effectiveness and reasonableness of the proposed coordinated planning model, which not only outperforms independent planning approaches but also effectively manages the uncertainties.
Empirical Evaluation of the Replacement of Conventionally Powered Vehicles with Hybrid and Electric Vehicles on the Example of the Poznań Agglomeration
Authors: Jagielski Aleks , Ziółkowski Andrzej , Bednarek Maciej , Siedlecki Maciej
Published: October 2024

From the collected data of the Central Register of Vehicles, an average of 15,000 vehicles were registered per month in the Greater Poland region in 2020–2022. It should be borne in mind that most of them were conventionally powered cars-spark-ignition or compression-ignition engines, the daily operation of which negatively affects the environment. Institutions responsible for regulating the homologation of passenger vehicles are introducing increasingly stringent emission standards to reduce the harmful effects of vehicles on the environment. In addition, more and more public campaigns are being conducted on the use of cars equipped with alternative propulsion systems, e.g., electric, full-hybrid or increasingly popular hydrogen-powered vehicles. The publication presents forecasts for the replacement of vehicles equipped with spark-ignition and compression-ignition engines with cars equipped with hybrid propulsion systems or electric systems. Tests of vehicles in real operating conditions were carried out in the Poznań agglomeration in an urban area, where the number of vehicles per area is higher than in the case of a nonurban zone. The research objects were both cars equipped with a conventional drivetrain and a hybrid system. The research made it possible to draw up a hybridization index, which was then applied to the number of vehicles registered over the past few years.
City Logistics Solutions for CO2 Emission Reduction and Energy Efficiency: A Comparative Study of Vitoria-Gasteiz, Tartu, and Sønderborg
Authors: Rut Joanna , Dziki Wiktoria , Beniak Ryszard , Podpora Michal , Gardecki Arkadiusz , Klin Bartlomiej
Published: October 2024

Modern cities face the challenge of reconciling dynamic development with environmental protection, as well as ensuring a high quality of life for their residents. In a time of continuous urbanization, with more than half of the world’s population living in cities, city logistics plays a crucial role in managing complex urban environments. As part of the concept of sustainable development, city logistics aims to minimize the negative impact of transport on the environment, while increasing operational efficiency and improving the comfort of life of residents in urban agglomerations. This article presents the results of a comparative analysis of the intensity of sustainable urban logistics development in selected cities of the European Union. It examines various strategies that reduce CO2 emissions, improve air quality, and increase building energy efficiency. The analysis demonstrates that cities investing in sustainable urban logistics not only improve their environmental performance but also increase their attractiveness and competitiveness as desirable places to live and work.
Experimental Investigation and Calculation of Convective Heat Transfer in Two-Component Gas–Liquid Flow Through Channels Packed with Metal Foams
Authors: Dyga Roman , Płaczek Małgorzata
Published: October 2024

This paper presents a study on heat transfer in two-phase mixtures (air–water and air–oil) flowing through heated horizontal channels filled with open-cell aluminum foams characterized by porosities of 92.9–94.3% and pore densities of 20, 30, and 40 PPI. The research included mass flux densities ranging from 2.82 to 284.7 kg/(m2·s) and heat flux densities from 5.3 to 35.7 kW/m2. The analysis examined the effects of flow conditions, fluid properties, and foam geometry on the intensity of heat transfer from the heated walls of the channel to the fluid. Results indicate that the heat transfer coefficient in two-component non-boiling flow exceeds that of single-phase flow, primarily due to fluid properties and velocities, with minimal impact from flow structures or foam geometry. An assessment of existing methods for predicting heat transfer coefficients in gas–liquid and boiling flows revealed significant discrepancies—up to several hundred percent—between measured and predicted values. To address these issues, a novel computational method was developed to accurately predict heat transfer coefficients for two-component non-boiling flow through metal foams.
Determinants of Forest Recreation Visitors’ Environmentally Responsible Behavior: The Moderating Role of Family Structure
Authors: Tseng Yun-Han , Ku Chih-Ming , Huang Wen-Shiung , Huang Chung-Ching , Wu Homer C. , Hsieh Chi-Ming
Published: October 2024

This research aims to verify the relationships between destination ij3dentity, environmental concern, and environmentally responsible behavior (ERB), based on the Cognition–Affect–Behavior (C–A–B) model, in the Sun-Link-Sea Forest Recreation Area (SLSFRA) in Taiwan. This study also aims to address the gap in understanding whether tourists’ family structure, with or without children, influences the impact of destination identity on their environmental concerns and ERB. A total of 431 samples were collected through convenience sampling and analyzed using structural equation modeling. The findings indicated that destination identity significantly influenced environmental concern, which in turn affected the general and specific ERB. Environmental concern acted as a mediator between destination identity and ERB. Specifically, tourists who strongly identified with the destination were more inclined to be concerned that its environmental condition influenced their travel experience, encouraging them to take action to protect the environment. Families with children who had greater environmental concern exhibited stronger general and specific environmentally responsible behavior (ERB) compared to families without children, who were more influenced by the impact of forest destination identity on their ERB. Practical implications for management and future research suggestions are proposed for relevant organizations and researchers.
Surface Treatment of Oak Wood with Silica Dioxide Nanoparticles and Paraloid B72
Authors: Mitani Andromachi , Kamperidou Vasiliki , Terzopoulou Paschalina
Published: October 2024

Wood is a valuable material with incomparable advantages, though it is susceptible to biotic and abiotic factors action that affect it adversely and shorten its service life. In the current study, the surface modification of oak wood is carried out through brief immersion in a solution of acrylic polymer Paraloid B72, in which silica dioxide nanoparticles in the form of nanopowder were dissolved at different contents (1, 2, 3, and 4% w/v of the solution) aiming at the elimination of wood material hygroscopicity, and the protection and improvement of other properties. Specifically, the modified and unmodified wood specimens were characterized in terms of physical characteristics (density, equilibrium moisture content, colour, and surface roughness), hygroscopic properties (swelling and absorption percentage) and accelerated weathering performance using xenon light and cycles of moisturizing and drying. The results revealed the dimensional stability of the samples and a significant increase in the hydrophobicity of the modified wood, as well as a significant increase in the resistance to the ageing/weathering factors of oak wood, which was proportional to the increase in the presence of nanoparticles in the Paraloid B72 solution. The colour of the treated samples slightly changed towards darker shades, more reddish and yellowish (with L* to decrease, while a* and b* to slightly increase), though the treated wood revealed higher colour stability. The surface roughness parameters (Ra, Rq, and Rz) increased significantly, restricting the wide application of the treated wood in indoor or outdoor applications where surface roughness constitutes a critical factor. The findings of the current work contribute not only to the production of longer-lasting wood and timber structures, but also to the conservation of the existing weathered heritage timber structures.
Systematic Literature Review of Barriers and Enablers to Implementing Food Informatics Technologies: Unlocking Agri-Food Chain Innovation
Authors: Orjuela-Garzon William Alejandro , Sandoval-Aldana Angélica , Mendez-Arteaga Jonh Jairo
Published: October 2024

Access to food products is becoming more and more complex due to population growth, climate change, political and economic instability, disruptions in the global value chain, as well as changes in consumption dynamics and food insecurity. Therefore, agri-food chains face increasingly greater challenges in responding to these dynamics, where the digitalization of agri-food systems has become an innovative alternative. However, efforts to adopt and use the technologies of the fourth industrial revolution (precision agriculture, smart agriculture, the Industrial Internet of Things, and the Internet of Food, among others) are still a challenge to improve efficiency in the links of production (cultivation), processing (food production), and final consumption, from the perspective of the implementation of Food Informatics technologies that improve traceability, authenticity, consumer confidence, and reduce fraud. This systematic literature review proposes the identification of barriers and enablers for the implementation of Food Informatics technologies in the links of the agri-food chain. The PRISMA methodology was implemented for the identification, screening, eligibility, and inclusion of articles from the Scopus and Clarivate databases. A total of 206 records were included in the in-depth analysis, through which a total of 34 barriers to the adoption of Food Informatics technologies (13 for the production link, 12 for the processing link, and 9 for the marketing link) and a total of 27 enablers (8 for the production link, 11 for the processing link, and 8 for the marketing link) were identified. Among the barriers analogous to the three links analyzed are privacy and information security and high investment and maintenance costs, while the analogous enablers are mainly government support.
Tuning the Interfacial Deformation of Gliadin-Flaxseed Gum Complex Particles for Improving the Foam Stability
Authors: Wu Ping , Shang Wei , Shao Jiaqi , Deng Qianchun , Zhou Jisong , Xiang Xia , Peng Dengfeng , Jin Weiping
Published: October 2024

Gliadin nanoparticle (GNP) is a promising foaming agent, but its application is hindered by the limited foam stability under low acidic conditions. Herein, we attempted to tune the foam stability of GNP by coating it with flaxseed gum (FG) and investigated the structure, interfacial behaviors, and foam functionality of gliadin-FG (GFG) particles at pH 4.5. Results showed that the formation of GFG complex particles was driven by an electrostatic interaction between positive charge patches on the surface of GNP (~17 mV) and negative charges in FG molecule (~−13 mV) at all tested ratios. The addition of appropriate amounts of FG (1:0.05) effectively improved the foam stability of GNP. This was because GFG with larger sizes and lower surface charge possessed higher rigidity after coating with FG. When they adsorbed at the air/water interface, their deformation process was slower than that of GNP, as indicated by interfacial dilatational rheology and cryo-SEM, and the covered particles seemed to be more closely distributed to form solid-like and dense interfacial films. Notably, the addition of FG at a higher ratio (1:0.3) promoted the foam stability of GNP by about five folds because the larger GFG with suitable flexibility and wettability could form a stiff interface layer with more significant elastic response, and the unabsorbed particles and FG could form a gel-like network structure in the continuous phase. These characteristics effectively prevented foam disproportionation and coalescence, as well as retard the drainage. Our findings demonstrate that coating GNPs with FG is an effective approach to improve their application in foamed foods.
Interaction of Val66Met Brain-Derived Neurotrophic Factor and 5-HTTLPR Serotonin Transporter Gene Polymorphisms with Lifetime Prevalence of Post-Traumatic Stress Disorder in Primary Care Patients
Authors: Guzman-Castillo Alejandra , Vicente Benjamín , Schmidt Kristin , Moraga-Escobar Esteban , Rojas-Ponce Romina , Lagos Paola , Macaya Ximena , Castillo-Navarrete Juan-Luis
Published: October 2024

Background/Objectives: Post-traumatic stress disorder (PTSD) is a complex condition influenced by both genetic and environmental factors. This longitudinal study aimed to explore the connection between two specific genetic polymorphisms, Val66Met and 5-HTTLPR, and the lifetime prevalence of PTSD in patients from primary care settings. We also examined the role of sociodemographic and psychosocial factors to provide a more comprehensive view of PTSD risk. Methods: We recruited a cohort of primary care patients and diagnosed PTSD using a standardized diagnostic interview. Genetic analyses focused on Val66Met and 5-HTTLPR polymorphisms. We applied logistic regression to assess the association between these genetic markers and PTSD, considering factors such as gender, family history of depression, and experiences of childhood maltreatment. Results: Our findings show that women, individuals with a family history of depression, and those exposed to childhood maltreatment have a higher risk of developing PTSD. While the Val66Met polymorphism was not significantly associated with PTSD, the 5-HTTLPR polymorphism showed a marginal relationship. No significant interaction was found between the two polymorphisms in relation to PTSD. Conclusions: This study underscores the multifactorial nature of PTSD, influenced by both genetic and environmental factors. The findings point to the importance of further research on genetic predispositions and highlight the value of early interventions for high-risk populations in primary care settings.
Genetic Features of Uveal Melanoma
Authors: Sorrentino Francesco Saverio , Culiersi Carola , Florido Antonio , De Nadai Katia , Adamo Ginevra Giovanna , Nasini Francesco , Vivarelli Chiara , Mura Marco , Parmeggiani Francesco
Published: October 2024

Background/Objectives: Although it comprises only 5% of all melanomas, uveal melanoma (UM) is the most commonly observed primary intraocular cancer. Methods: Poor patient survival persists in spite of innovative systemic therapies. In fact, approximately fifty percent of UM patients develop metastases from micro-metastases that remain undetected at the exact time of diagnosis. Results: The molecular understanding of UM is significantly enhanced by the recent identification of several mutations that are responsible for the metastasis, growth, and survival of UM. The crucial point is a more accurate genetic analysis for patient follow-up and metastatic risk prediction. Conclusions: This review provides a brief summary of the molecular features of UM that are recently discovered, as well as cytogenetic markers and biochemical pathways that are associated with the development of UM metastases.
Optical Genome Mapping for Detection of BCR::ABL1—Another Tool in Our Toolbox
Authors: Tang Zhenya , Wang Wei , Toruner Gokce A. , Hu Shimin , Fang Hong , Xu Jie , You M. James , Medeiros L. Jeffrey , Khoury Joseph D. , Tang Guilin
Published: October 2024

Background: BCR::ABL1 fusion is mostly derived from a reciprocal translocation t(9;22)(q34.1;q11.2) and is rarely caused by insertion. Various methods have been used for the detection of t(9;22)/BCR::ABL1, such as G-banded chromosomal analysis, fluorescence in situ hybridization (FISH), quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) and optical genome mapping (OGM). Understanding the strengths and limitations of each method is essential for the selection of appropriate method(s) of disease diagnosis and/or during the follow-up. Methods: We compared the results of OGM, chromosomal analysis, FISH, and/or RT-PCR in 12 cases with BCR::ABL1. Results: BCR:ABL1 was detected by FISH and RT-PCR in all 12 cases. One case with ins(22;9)/BCR::ABL1 was cryptic by chromosomal analysis and nearly missed by OGM. Atypical FISH signal patterns were observed in five cases, suggesting additional chromosomal aberrations involving chromosomes 9 and/or 22. RT-PCR identified the transcript isoforms p210 and p190 in seven and five cases, respectively. Chromosomal analysis revealed additional chromosomal aberrations in seven cases. OGM identified extra cytogenomic abnormalities in 10 cases, including chromoanagenesis and IKZF1 deletion, which were only detected by OGM. Conclusions: FISH offers rapid and definitive detection of BCR::ABL1 fusion, while OGM provides a comprehensive cytogenomic analysis. In scenarios where OGM is feasible, chromosomal analysis and RT-PCR may not offer additional diagnostic value.
Rugose Coral Biogeography of the Western Palaeotethys During the Mississippian
Authors: Rodríguez-Castro Isabel , Rodríguez Sergio
Published: October 2024

The Mississippian was an epoch of strong earth system changes, both tectonic and climatic. During the Mississippian, the marine faunas experienced a recovery after the late Devonian mass extinctions, and the rugose corals are a conspicuous example. This study tries to give a general view of the utility of rugose coral to reconstruct the palaeogeography in the Western Palaeotethys during the Mississippian. The methodology includes a database with the genera and species recorded in that area and time period, compiled using more than 700 articles and revisions of several collections in Europe. We worked with the six sub-provinces defined in previous studies for the Western Palaeotethys. A generic-level analysis was performed using paired group hierarchical clustering, building clusters for the Tournaisian, early Visean, late Visean and Serpukhovian. With that information, palaeomaps for those intervals have been illustrated and discussed. The rugose corals have some deficits for the reconstruction of the biogeography because of their strong palaeoecologic control and their insufficient and unequal record, but they provide important information that improves the knowledge on the palaeogeography of the studied region.
Efficacy of Horticultural Therapy on Positive, Negative, and Affective Symptoms in Individuals with Schizophrenia: A Systematic Review and Meta-Analysis of Randomized Controlled Trials
Authors: Lee Yi-Wen , Chen Tzu-Ting , Hsu Chih-Wei , Chen Ming-De , Lin Pao-Yen , Huang Yu-Chi , Hung Chi-Fa , Chen Chyi-Rong
Published: October 2024

Background/Objectives: Positive symptoms, negative symptoms, and emotional disturbances are core features of schizophrenia. Although horticultural therapy (HT) has shown promise as an adjunctive treatment, evidence supporting its effectiveness remains limited. This systematic review and meta-analysis aimed to assess the impact of HT on total symptoms, positive symptoms, negative symptoms, and emotional disturbances in individuals with schizophrenia. Methods: We conducted a search for randomized controlled trials (RCTs) published up to March 2024 across multiple databases, including PubMed, Embase, Cochrane Library, CINAHL, CEPS, CNKI, Wanfang, and Yiigle. A random-effects model was employed to calculate the standardized mean difference (SMD). Results: A total of 35 studies enrolling 2899 participants were included. Our results indicated that, in the short term (≦3 months), HT has moderate to large effect sizes on total symptoms (SMD = 0.690, 95% CI 0.463 to 0.916), positive symptoms (SMD = 0.695, 95% CI 0.038 to 1.351), negative symptoms (SMD = 0.681, 95% CI 0.395 to 0.967), depression (SMD = 0.646, 95% CI 0.334 to 0.959), and anxiety (SMD = 0.627, 95% CI 0.364 to 0.890), with more pronounced benefits for anxiety symptoms in patients with a shorter duration of illness. In the long term (>3 months), HT shows large effect sizes for total symptoms (SMD = 1.393, 95% CI 0.858 to 1.928), negative symptoms (SMD = 1.389, 95% CI 0.935 to 1.842), anxiety (SMD = 1.541, 95% CI 1.042 to 2.040), and moderate to large effect sizes for positive symptoms (SMD = 0.667, 95% CI 0.077 to 1.258) and depression (SMD = 0.707, 95% CI 0.198 to 1.217). Additionally, longer weekly treatment durations are associated with better outcomes for total symptoms and negative symptoms. Schizophrenia patients with more severe initial symptoms may be potential responders to HT. Conclusions: These findings support the efficacy of HT in improving symptoms and emotional well-being in schizophrenia patients. Further trials with more rigorous designs are warranted to confirm these benefits.
Fasting and Diet: Overview in Chronic Lymphocytic Leukemia
Authors: Trojani Alessandra , Bossi Luca Emanuele , Cairoli Roberto
Published: October 2024

Background: This review aims to provide an overview of the potential impact of fasting and diet on cancer, and in particular, on chronic lymphocytic leukemia (CLL), which is the most frequent form of leukemia in the Western world. Methods: Experimental and clinical studies have provided evidence of the crucial role of fasting in enhancing cancer treatment and improving outcomes for oncological patients, particularly at the early stages of the disease. Results: Emerging evidence highlights that fasting creates a differential stress response under critical conditions by fostering the survival of normal cells while limiting the survival and growth of cancer cells. Pivotal studies on CLL have highlighted the potential of fasting and dietary components to influence the stromal microenvironment and certain metabolic pathways, thereby affecting cancer cell apoptosis and immune response. In addition, explorative and initial clinical studies suggest that fasting and specific diets can mitigate the toxicity of chemotherapy. Conclusions: Clinical trials are needed to evaluate the efficacy and safety of nutritional and fasting approaches in cancer and CLL. Future investigations could provide new insights into the potential role of diet and fasting in the prevention and treatment of cancer, potentially leading to more effective and personalized therapeutic strategies.
Reviving the Practices of Transhumance in a Forgotten Settlement in Mainland Greece
Authors: Tzortzi Julia Nerantzia , Theodoridou Sofia , Koukosoula Styliani
Published: October 2024

The aim of this research project is the investigation of the forgotten landscape of transhumance in mainland Greece, focusing on areas that have been abandoned by modern society and their historical significance. The goal is to make these lands functional again by preserving their cultural heritage and developing a sustainable community that meets the needs of people, livestock, and the environment. The investigation employs a dual approach: examining transhumance as a cultural and natural practice while analyzing vernacular architecture to preserve collective memory. This involves extensive historical research and a simultaneous analysis of spatial, social, symbolic, and architectural elements. This study identifies points of convergence between cultural practices and architectural heritage, applying these insights to contemporary redevelopment scenarios. Findings reveal a significant interplay between transhumance practices and the region’s anonymous architecture. Key characteristics of the landscape and its spatial identity are documented, providing insights into effective preservation and redevelopment strategies. Specific case studies validate these strategies, demonstrating their relevance to current and future efforts. This study concludes that understanding the historical context and spatial identity of transhumance landscapes is crucial for sustainable redevelopment. By preserving cultural practices and architectural heritage, this project provides a framework for revitalizing these landscapes, ensuring a balanced coexistence of people, livestock, and the environment.
Dynamic Changes in Polyphenols in Fruit Development of Red Flesh Apple ‘Hongxun 2’
Authors: Wang Dajiang , Wang Guangyi , Lu Xiang , Liu Zhao , Sun Simiao , Guo Hanxin , Tian Wen , Li Zichen , Wang Lin , Li Lianwen , Gao Yuan , Wang Kun
Published: October 2024

In this study, fruits of the red flesh Malus plant ‘Hongxun 2’ (Malus neidzwetzkyana (Dieck) Langenf.) and green flesh Malus plant ‘Xinye 13-11’ (Malus sieversii (Led.) Roem.) were used as experimental materials. Both of them came from Xinjiang, China, and Malus neidzwetzkyana (Dieck) Langenf. is believed to be a variant of Malus sieversii (Led.) Roem. The components and contents of polyphenols in the peel and pulp of the two kinds of fruit during the development period were detected, and the dynamic changes and differences in the polyphenols between the two kinds of fruit were discussed. The results showed that the total polyphenol content of ‘Xinye 13-11’ was higher in the peel and pulp than that of ‘Hongxun 2’, and the content of peel was higher than that of pulp in the two kinds of fruit. An analysis of five types of polyphenols showed that anthocyanins were only contained in the peel and pulp of ‘Hongxun-2’, and the peel had a higher content than the pulp. Cyanidin 3-O-galactoside was the main anthocyanin component. Four other types of substances, except hydroxycinnamics, were higher in ‘Hongxun-2’ than ‘Xinye 13-11’, while the contents of other substances in ‘Xinye 13-11’ were higher than those of ‘Hongxun 2’. The accumulation of major polyphenol components in the peel and flesh of ‘Hongxun 2’ and ‘Xinye 13-11’ apples was significant in the period before and after 65 days after flowering, and the contents of procyanidin B1 and procyanidin C1 were the highest in this period. In addition to the difference in anthocyanin content between ‘Hongxun 2’ and ‘Xinye 13-11’, the chlorogenic acid content in the peel and pulp of ‘Hongxun 2’ was significantly higher than that of ‘Xinye 13-11’, and the contents of other components were lower than those of ‘Xinye 13-11’. Moreover, based on the components and contents of polyphenol components, this paper supports the viewpoint that Malus neidzwetzkyana (Dieck) Langenf is a separate species to Malus sieversii (Led.) Roem.
Comparative Hydrogen Production Routes via Steam Methane Reforming and Chemical Looping Reforming of Natural Gas as Feedstock
Authors: Mohd Yunus Salmi , Yusup Suzana , Johari Siti Sorfina , Mohd Afandi Nurfanizan , Manap Abreeza , Mohamed Hassan
Published: October 2024

Hydrogen production is essential in the transition to sustainable energy. This study examines two hydrogen production routes, steam methane reforming (SMR) and chemical looping reforming (CLR), both using raw natural gas as feedstock. SMR, the most commonly used industrial process, involves reacting methane with steam to produce hydrogen, carbon monoxide, and carbon dioxide. In contrast, CLR uses a metal oxide as an oxygen carrier to facilitate hydrogen production without generating additional carbon dioxide. Simulations conducted using Aspen HYSYS analyzed each method’s performance and energy consumption. The results show that SMR achieved 99.98% hydrogen purity, whereas CLR produced 99.97% purity. An energy analysis revealed that CLR requires 31% less energy than SMR, likely due to the absence of low- and high-temperature water–gas shift units. Overall, the findings suggest that CLR offers substantial advantages over SMR, including lower energy consumption and the production of cleaner hydrogen, free from carbon dioxide generated during the water–gas shift process.
Sources of Environmental Reinforcement and Engagement in Health Risk Behaviors Among a General Population Sample of US Adults
Authors: L’Insalata Alexa M. , Girard Jeffrey M. , Fazzino Tera L.
Published: October 2024

Research supports the premise that greater substance use is associated with fewer sources of environmental reinforcement. However, it remains unclear whether types of environmental reinforcement (e.g., social or work) may differentially influence use. This study tested the association between types of environmental reinforcement and engagement in multiple health risk behaviors (alcohol use, binge eating, and nicotine use). Cross-sectional data were collected from a general population sample of US adults (N = 596). The Pleasant Events Schedule (PES) was used to measure sources of reinforcement. Exploratory structural equation modeling (ESEM) characterized different areas of environmental reinforcement and correlations with alcohol consumption, binge eating, and nicotine use. A four-factor structure of the PES demonstrated a conceptually cohesive model with acceptable fit and partial strict invariance. Social-related reinforcement was positively associated with alcohol consumption (β = 0.30, p < 0.001) and binge eating (β = 0.26, p < 0.001). Work/school-related reinforcement was negatively associated with binge eating (β = −0.14, p = 0.006). No areas of reinforcement were significantly associated with nicotine use (p values = 0.069 to 0.755). Social-related activities may be associated with engagement in multiple health risk behaviors (more binge eating and alcohol use), whereas work/school-related activities may be preventative against binge eating. Understanding these relationships can inform prevention efforts targeting health risk behaviors.