bluuebunny/crossref_metadata_2025 huggingface dataset:
Ecojustice Education Approach: Using Montes’ “Turtle Season” Short Story to Teach the Intersectionality of Gender and Environment
Authors:   Carlos Hilado Memorial State College, Bayona Abegail , Adorador Sensei ,   Polytechnic University of the Philippines
Published: January 2025

This paper uses Ecojustice Education as advanced by Martusewicz, Edmundson, and Lupinacci (2015) as an approach to use literature as material to teach the intersectionality of gender and environment through a constructivist module form. Studies including Seager (1999) reveal the link between patriarchal methods and the destruction of nature, illustrating a space where gender politics exacerbates environmental degradation. This paper presents a model for maximizing literature as a reflection of significant human experiences. Here the researchers crafted a way to use literature to marry issues of gender and environment in a palatable form for classroom consumption. The researchers see the model as useful for developing instructional materials that brings together issues tackled by gender and development and environmental education. Through the Ecojustice Education approach, literature is perused not as art for art’s sake but rather elevated as a platform to promote an ecological worldview to shape student consciousness and elicit active efforts from schools and educators to be creative in addressing concerns of gender and environment.
Adolescent Friendly Sexual and Reproductive Health Service Delivery in Kaski District, Nepal
Authors: Bhattarai Anil
Published: January 2025

Adolescents (10-19 years old) account for nearly a quarter (approximately 6.4 million or 24.2%) of Nepal’s population. The Government of Nepal had identified adolescents as an important and underserved population, and critical to achieving national health and development goals. This study aimed to add up the insights whether the sexual and reproductive health services provided to adolescents are friendly as par the national standards and in the adolescent’s perspectives as well. A descriptive cross sectional study using both quantitative and qualitative approaches was conducted in Kaski district where eight Adolescent Friendly Health Facilities (AFHFs) were selected randomly and the schools in the proximity of the selected health facilities were selected to collect adolescents’ perspectives. Structured questionnaire was used to collect data from school adolescents between the ages 15-19 years. Descriptive analysis was carried out to summarize the socio-demographic characteristics, health facility related characteristics, and observation findings of the health facility. The study found that among the total adolescents participated in the study 69% had utilized the health service in last six month of which 82.9% had visited to AFHFs, 35% of them had to travel more than 30 minute distance to reach nearby AFHFs, 67.7% school adolescents had knowledge on the availability of AFHS in the health facility. This study found the status of adolescent friendly health service delivery in Kaski district was below the certification criteria of adolescent friendliness.
Impact of Adult Acne in Quality of Life by using DLQI (Dermatology Life Quality Index)
Authors: Paudel Rosina , Keshar Khadka Dhan , Rijal Arpana
Published: January 2025

Acne is a chronic inflammatory disease of pilosebaceous unit. It is one of the most common diseases of adolescence, seen in 80% of young people. Adult acne seems to be a common problem and there has been an increase in prevalence of adult acne in last two decades. This study determined the impact of adult acne in Quality of life by using DLQI (Dermatology Life Quality Index). It is a hospital based descriptive cross-sectional study in the department (OPD), Department of Dermatology and Venereology at B. P. Koirala Institute of Health Sciences, Dharan. The sample size N=161 where data has been collected with the help of purposive sampling technique. Quality of life was measured in adult acne patients, using the Nepali version of dermatology life quality index (DLQI) questionnaire. Data has been summarized using frequency distribution tables and graphical methods of presentation of data. Bi-variate analysis for association was done using appropriate test of significance. Multivariable binary logistic regression was then further done to find the adjusted odds ratio for the determinants. Statistical significance was tested with 95% confidence interval and p value less than 0.05 was considered significant. Adult acne has shown to affect quality of life moderately, with greater effect among females. Severity of acne was found to have significant association with smoking, sun exposure and intake of milk products. This study being an observational study needs careful interpretation of the associated factors. The findings from this study warrants need for interventions targeting for behavioural and dietary modification to reduce the severity of acne, and its impact on quality of life.
ARTIFICIAL INTELLIGENCE DRIVEN DRONE OBSERVATION AND PEST CONTROL IN BANANA CROP: A SYSTEMATIC REVIEW
Authors: Shahzad Nasim  , Munaf Rashid  , Saba Yousha
Published: January 2025

Bananas are the most commonly eaten and significant fruit in global trade. Bananas are produced using a variety of methods and environments. In addition to regularly updating farmers on problems in banana plant leaves, this system aims to discover, diagnose, and treat banana leaf diseases. Customers' wants and lifestyles have changed significantly during the past few decades. These modifications provide additional difficulties for farmers whose output must satisfy consumer needs. Both the farmer and the consumer will benefit from the capacity to categorize agricultural products according to size and quality. In this case, the system gets its input in the form of standard photos of banana leaves taken using various image capture devices. It will then process those photos to identify any diseases and alert the farmer. Additionally, the system will advise the farmer on what to do next, including which fertilizers, herbicides, and agricultural practices to employ in order to prevent illnesses from harming neighboring crops. In this systematic review, useful and efficient methods for identification are presented in works that fall under the categories of image classification, AI/ML, deep learning, and mobile applications.
STUDY ON THE HEAT TOLERANCE IN TOMATO (LYCOPERSICONE SCULENTUM MILL.) AT HYDERABAD, SINDH
Authors: Muzamil Hussain Awan  , Asif Ali Kaleri  , Saba Solangi  , Faiza Bibi  , Nabeela Rashid  , Jawad Ahmed  , Noman Asghar  , Ahmed Ali  , Abdul Sattar  , Duaa Akbar
Published: January 2025

Present study was conducted to analyse the heat tolerance and correlation estiamtes in Tomato (lycopersicon esculentum Mill.) at Hyderabad, Sindh. The data was collected for membrane thermostability, elongation of stigma and antheridial cone spiliting, flowers no. and number of flowers shed per plant and production per plant was under the study. The data revealed significant differences among the studied traits. Among the genotypes, (1) Fanto F1 Hybrid exhibited the highest heat tolerance, followed by was (2) Hybrid tomato HT-101B, as evidenced by their high membrane thermostability and low number of flowers shed. These genotypes also demonstrated the highest fruit yield during the hot period. The analysis showed a positive association between fruit yield and membrane thermostability, while a negative association was observed with the number of flowers shed, stigma tube elongation, and antheridial cone splitting. In summary, Roma, with the lowest membrane thermostability, was found to be the most sensitive genotype to heat stress.
Konsep Perencanaan Dalam Manajemen Pendidikan
Authors: Usep Suherman  , Esya Esya Mulyani , Eliva Sukma Cipta
Published: January 2025

Plans or planning are needed in various forms of organization, because this planning is a basic management process in making decisions and taking actions. Planning is needed in all types of activities, be it organizational activities, companies or activities in the community, and planning exists in every management function, because these functions can only carry out decisions that have been determined in planning. This article aims to reveal the importance of planning in the education management system. The research method used is a literature study. The results of this study reveal that planning is the process of defining organizational goals, producing management art to achieve goals, and planning organizational work activities. Planning is also the most important process in the principle of all management functions, because without planning other functions of organizing, directing, and controlling. Good planning will form good management. We recommend that in reaching decisions and actions in various forms of organization use the basic management process in the form of planning. In a plan, it is necessary to pay attention to the nature of good planning to achieve the desired results.
Pengaruh Model Pembelajaran Teams Games Tournament Terhadap Kemampuan Psikomotorik Peserta Didik Pada Mata Pelajaran PAI di MAN 1 Pamekasan
Authors: Abd Mannan  , Leli Lestari  , Dwi Yanti Margareta  , Hendra Maulana
Published: January 2025

Psychomotor skills are a prerequisite for achieving success in learning PAI (Islamic Education), as the outcomes of psychomotor learning are a continuation of cognitive and affective learning outcomes, which become apparent when students exhibit certain behaviors or actions according to the meanings in these two domains in daily life. This study employs a quantitative approach. The aim of this research is to determine whether there is an effect of the Teams Games Tournament (TGT) learning model on students' psychomotor skills in PAI subjects at MAN 1 Pamekasan. The study uses a quantitative approach. Data collection techniques involve using questionnaires to gather information on the TGT learning model and students' psychomotor skills. Data are analyzed using the product-moment correlation formula. The results indicate an effect of the Teams Games Tournament learning model on students' psychomotor skills in PAI subjects at MAN 1 Pamekasan. This is evident from the analysis using the product moment formula, which shows that the product moment "r" value of 0.565 falls between 0.41 and 0.70, with a moderate interpretation. This means there is a moderate effect of the Teams Games Tournament learning model on students' psychomotor skills in PAI subjects at MAN 1 Pamekasan, with a sufficient contribution
Komunikasi Efektif dalam Proses Pembelajaran
Authors: Ahmad Fahroni
Published: January 2025

Effective communication in learning is key to enhancing the quality of the teaching and learning process. However, various communication barriers often hinder interactions between teachers and students, making it challenging to achieve learning objectives. This article aims to examine the role of communication in the educational context, with a focus on teacher-student interactions. The study employs a library research approach to analyze relevant literature. The findings indicate that effective communication encompasses openness, empathy, supportiveness, positive attitudes, and equality, all of which contribute to creating an optimal learning environment. Open communication models, such as dialogical and persuasive approaches, can help students better understand learning materials. Additionally, the utilization of information technology and learning media enhances the success of communication in education. By paying attention to factors such as the communicator, the communicant, and the message, teachers are expected to create an interactive learning environment that supports the achievement of educational goals.
Ustawy regulujące ustrój związków metropolitalnych a publiczny transport zbiorowy – praca prawnoporównawcza
Authors:   Uniwersytet im. Adama Mickiewicza w Poznaniu, Wydział Prawa i Administracji, Misiejko Adrian
Published: January 2025

Przedmiotem niniejszego artykułu jest porównanie prawnych regulacji organizowania publicznego transportu zbiorowego ustanowionych na gruncie ustawy o związkach metropolitalnych (już nieobowiązującej) oraz ustawy o związku metropolitalnym w województwie śląskim. Autor w pierwszej kolejności omawia przepisy ustawy o związkach metropolitalnych, wskazując na wprowadzone rozwiązania oraz związane z nimi wątpliwości. W drugiej części pracy omówione zostały prawne aspekty organizacji publicznego transportu zbiorowego pod rządami obowiązującej ustawy o związku metropolitalnym w województwie śląskim. Autor dokonał porównania najważniejszych instytucji prawnych, czego efektem jest ustalenie, w jakich sferach nowa ustawa prowadzi do rozwiązania wątpliwości prawnych, a w jakich – prowadzi do powstania nowych. Efektem tych rozważań jest krytyczna ocena ustawy o związku metropolitalnym w województwie śląskim.
ICTs and innovation in hotel management
Authors: Palacios-Marqués Daniel , Ballester Miquel Jose Carlos , Perez-Ruiz Pilar , Hernandez-Gadea Javier
Published: January 2025

Objective: The objective of this article is to describe the relationship between service innovation and ICTs and how they have influenced the hotel sector.
Methodology: We used exploratory factor analysis (EFA) to describe the causal relationships. A total of 280 surveys were conducted with managers of Spanish 4 and 5-star hotels to test the 3 hypotheses using partial least squares structural equation modelling. This non-parametric tool exempts the sample from conforming to the normal distribution.
Results: The authors have shown how the relationship between service innovation and ICTs is established through the ability to learn so that hotels can increase their productivity and the results obtained. The incorporation of new technologies in the customer experience and the digitisation of internal processes are mainly highlighted here.
Limitations: Of interest for the hotel sector is the causality of information technologies as a driver of innovative development, as well as the mediation of the learning capacity of organisations as an influential aspect in their development.
Practical implications: This article describes the influence of ICTs and learning capacity as an enabler of service innovation capacity in hotels.
Vistas aéreas, archivo y políticas de producción de verdad. Sobre el uso de las imágenes en Gas Lacrimógeno en Plaza de la Dignidad de Arquitectura Forense
Authors: Ayala Valdes Carla Nicole
Published: January 2025

El artículo analiza las estrategias visuales y discursivas empleadas por el colectivo interdisciplinario Arquitectura Forense (AF) en el desarrollo del material audiovisual Gas Lacrimógeno en Plaza de la Dignidad (2020). Esta investigación buscó evidenciar el uso excesivo de gases químicos por parte de Carabineros de Chile contra la masa protestante en el margen de la revuelta social del 2019. Desde la perspectiva de los estudios visuales, el texto se centra en el rastreo, uso y montaje de imágenes captadas por dispositivos de visión cenital, como drones y satélites, utilizadas por AF para construir un testimonio visual. Se propone analizar estas imágenes como un complejo digital que combina documentación, archivo e información incorpórea y maquínica, y que logra operar como engranaje político de producción de verdad, al registrar desde el aire diversas formas de conflictos geopolíticos contemporáneos.
No-ver corporal, no-ver mediático y no-ver público en las prácticas artivistas del Estallido Social de Chile (2019)
Authors: Alfonso Bouhaben Miguel
Published: January 2025

Durante el estallido social chileno proliferaron prácticas artivistas que criticaban dos tipos de violencia estatal: la crítica a la violencia física contra el sujeto plural de la revuelta, que sufría el ataque de las bombas lacrimógenas contra sus ojos; y la crítica a la violencia semiótica, expresada en las tergiversaciones de la televisión y en las prácticas de invisibilización de las expresiones ciudadanas en monumentos y muros. La presente investigación tiene por objeto el análisis de un corpus de dichas prácticas artivistas a partir de tres ejes: a) el no-ver corporal: análisis de las prácticas artivistas que critican las formas estatales de invisibilización del cuerpo de los manifestantes; b) el no-ver mediático: análisis de las prácticas artivistas que critican las formas de invisibilización de las imágenes de los manifestantes en los medios de comunicación dominantes; y c) el no-ver público: análisis de las prácticas artivistas que critican las formas estatales de invisibilización de los grafitis, afiches y eslóganes inscritos en las paredes de los museos, monumentos y edificios. Estas prácticas artivistas visibilizan el disenso invisibilizado frente a las formas de consenso hegemónicas expresando así una guerra visual por el relato: una Imagomaquia.
Legitimación y deslegitimación de la violencia policial mediante racionalización en Twitter: el caso del paro nacional universitario en Colombia de 2018
Authors: Tutkal Serhat
Published: January 2025

Este artículo examina cómo las referencias a la racionalidad y la razón se utilizan en las redes sociales para legitimar o deslegitimar la violencia policial colombiana contra lxs manifestantes. Para ello, analiza 8,421 tuits, recogidos a partir del hashtag #FuerzaEstebanMosquera, en los que se legitima o deslegitima la violencia policial en la Universidad del Cauca en diciembre de 2018. Ubicada dentro de los Estudios Críticos del Discurso (ECD), esta investigación se centra especialmente en los mecanismos discursivos y las referencias culturales específicas que se utilizaron en estos tuits. Conceptualizando la legitimidad e ilegitimidad de la violencia como un estado fluido en lugar de una categorización fija, esta investigación sugiere que la legitimación y deslegitimación de la violencia son procesos continuos, lo que significa que la legitimidad o ilegitimidad de un acto violento puede transformarse haciendo referencias culturales y utilizando estrategias discursivas adecuadas al contexto. Al examinar los discursos en las redes sociales sobre la legitimidad e ilegitimidad percibidas de la violencia policial, este artículo pretende contribuir a idear formas de promover una cultura de paz y no violencia
Pueblo, emergencia popular y democracia: categorías disputadas
Authors: Friz Cristóbal
Published: January 2025

El presente artículo ofrece una reflexión sobre los alcances políticos de los denominados “estallidos sociales”, a partir de las posibles relaciones que cabe entablar entre los “estallidos sociales”, entendidos como procesos de emergencia popular, el “pueblo” y la “democracia”. ¿Se puede considerar a los estallidos sociales como procesos de emergencia popular? En caso de ser así, ¿cabe pensarlos como procesos de profundización democrática, entendiendo democracia como régimen de soberanía del pueblo? La reflexión propuesta tiene por referencia y motivación al ciclo de movilizaciones de octubre de 2019 en Chile. Es por este motivo que revisa principalmente textos sobre dicho proceso, los que son complementados con fuentes que permiten enfocar más adecuadamente los vínculos entre pueblo, emergencia y democracia. El propósito del ensayo es poner de manifiesto que los rendimientos políticos de los procesos de emergencia constituyen una cuestión disputada, sujeta a la pugna de interpretaciones. Se procura mostrar dicha pugna a partir del carácter controversial de los conceptos “pueblo”, “emergencia” y “democracia”, y de los vínculos que se puede entablar entre ellos.
La mensajera vegetal, un universo sonoro khipunk andino
Authors: Moscoso Mafe
Published: January 2025

La crisálida agave es un vegetal, pero "no sólo". Forma parte del universo khipunk, creado por JoséLuis Jácome Guerrero quien, en colaboración con otras/os artistas ecuatorianos/as, teje una suerte de epistemología cósmica. Su trabajo, anclado en la ciencia ficción andina,gira alrededor de la crisálida agave que es una entidad que disuelve la realidad por medio de, al menos tres gestos que este artículo propone explorar: 1. El khipunk como una entidad vegetal del entremedio que encarna la/el nueva/o mestiza que habita la herida colonial con el fin de revertirla 2. La materialización de la mensajera vegetal, a través de los khipus, en sonido cuya escucha precisa de una liberación sensorial 3. El khipu como objeto mágico que ponen en evidencia los límites de los métodos de investigación occidentales y como oráculo andino cuyas emisiones noson perceptibles sin replantear las concepciones hegemónicas sobre lo que significa abrir la mirada.
Intervención monumental en Chile: museos vivos en tiempos de movilización social
Authors: Badilla Rajevic Manuela
Published: January 2025

El 18 de octubre de 2019 se inició una de las movilizaciones sociales más masivas en la historia de Chile, transformando el escenario político del país y desencadenando un fenómeno menos esperado, una oleada de alteraciones monumentales caracterizada por el cuestionamiento, intervención, creación y en algunos casos destrucción de los monumentos públicos de Chile. Si bien este fenómeno no es nuevo en el mundo, en Chile se expresó en una magnitud nunca vista, con más del 64% de todos los monumentos del país intervenidos. A partir de un proyecto de investigación que incluye 45 entrevistas con personas que participaron en las protestas y un trabajo de archivo que considera documentos históricos, de prensa y de redes sociales sobre los monumentos intervenidos, este trabajo sostiene que las intervenciones de monumentos conformaron verdaderos museos vivos. Se analizan tres características de las intervenciones a monumentos consideradas claves para producir una experiencia museográfica diferente a la tradicional: la movilización de memorias nacionales y locales, el tono afectivo intenso producido con las intervenciones y la apropiación del espacio urbano. El artículo finaliza con una reflexión sobre el potencial de la intervención monumental y sus huellas una vez que los periodos de convulsión social concluyen.
Crítica y verdad. Resistencias a la teoría en el campo crítico literario español del siglo XX
Authors: Pino Estivill Ester
Published: January 2025

Este artículo estudia la circulación de la teoría literaria en el campo literario español desde los años cuarenta, cuando se impuso la dictadura franquista, hasta los años ochenta, cuando la universidad española acabó por integrar un método estructuralista amputado de su potencia de transformación. Para ello, realizamos una comparativa entre los desarrollos de la teoría en el campo intelectual francés y su circulación en el campo crítico literario español que, por un lado, comprende la producción editorial y, por el otro, analiza las resistencias y obturaciones que encontró el pensamiento de la deconstrucción en la academia española. De la misma manera que el régimen franquista controlaba no solo lo que se comía sino también lo que se decía, veremos que hubo un racionamiento de la teoría que redujo sus alcances, así como un estraperlo que intentó introducir algunas de sus posibilidades políticas. En ese debate se sitúa la pregunta por la herencia que arrastran los estudios literarios en España en la actualidad.
Saint Catherine through the Lens of Feminism?
Authors: Benvenuto Carmelo Nicolò
Published: January 2025

Saint Catherine of Alexandria has been defined by modern scholarship as a “Ghost Saint”, for her possible identification of the hagiographical figure as a hypostasis of the Late-Antique philosopher, Hypatia of Alexandria. Since Hypatia herself has been often interpreted as a “feminist” or “proto-feminist” icon, one could wonder if and to what extent a Female Studies’ perspective would be useful to let modern scholars understand Catherine’s figure in herself. The aim of this paper will be to propose some possible interpretations of Saint Catherine’s functions through the lens of Female Studies, trying to disentangle in a diachronic approach some of the most significant moments of her literary and erudite representation, focusing in particular on the key-moment of its early modern reception.
Women in Early Christianity. Introduction
Authors: Dell’Isola Maria , Resta Mario
Published: January 2025

Introduccion to Early Christianity section
Delay techniques and delay technologies: Amazonian waters transindividuation records
Authors: Falci Carlos Henrique , Bergamo Marilia Lyra
Published: January 2025

This paper presents transindividuation records and observations, which the authors argue allow the perception of delay technologies. This study will present the ideas behind such descriptions and how they contribute to what can be considered a non-essentialist concept of relation. To navigate those ideas, the authors present three examples of transindividuation records documented during on-site research in the communities of Boca do Mamirauá, Caburini, Bom Jesus da Ponta da Castanha, São Francisco do Bauãna, Vila Sião, in Amazon, Brazil, during the period of eight months. This study demonstrates that the perception of time may change when we are in the presence of the waters of Amazon rivers. In relation to Amazon's waters, humans perceive a delay due to different orders of magnitude in nature, resulting in a specific mode of existence.
La comunicación interna, motor del bienestar emocional en el sector público
Authors: Compte Pujol Marc
Published: January 2025

La salud mental y el bienestar emocional son dos conceptos que van de la mano para comprender cómo nos sentimos, pensamos y actuamos en nuestra vida diaria. Representan un estado de armonía donde una persona reconoce sus capacidades, y puede desarrollar sus habilidades cognitivas y emocionales, vivir en sociedad y afrontar las exigencias del día a día. No se trata solo de evitar una dolencia mental, sino de ser capaz de gestionar los desafíos cotidianos, el estrés y mantener relaciones satisfactorias y un trabajo productivo.
Equipos multidisciplinares de desarrollo de producto digital
Authors: Latorre Dani
Published: January 2025

Desde hace años, lo habitual en el mundo de la construcción de software y productos digitales ha sido tener equipos organizados por gremios. Sin embargo, en los últimos tiempos, hemos ido viendo una creciente tendencia a trabajar organizados en equipos multidisciplinares. Esto es, veníamos de estructuras en las que había equipos de desarrollo, de diseño, de analistas, de QA, de gestión de proyectos, de sistemas, de business intelligence, etc. Incluso, mientras construir software se volvía más complicado, podían estar divididos por subgremios todavía más especializados, por ejemplo, equipos de frontend y backend en el desarrollo o de research y UI en el diseño.
Marketing auteurism: the vanishing industry model behind Pedro Almodóvar’s success in the US
Authors: Green Jennifer
Published: January 2025

Objective: To establish a model and then consider whether that model will continue to function in the future. Almodóvar’s typical production-distribution structure and exportable filmmaking style are outlined, as are his US distributor’s strategic use of specialised press, elite events and targeted marketing of arthouse audiences with a specific cultural capital and an established view of European cinema.
Methodology: The article focuses on qualitative research, supported by quantitative box office data, to analyse Almodóvar’s reach in the US and to study influences and trends in the North American market. Relevant interdisciplinary theoretical frameworks are applied to explain and contextualise the data.
Results: The article finds clear patterns of production, distribution and marketing that constitute a model built around Almodóvar’s status as an auteur, and it finds that all elements comprising that model are currently in flux, as are US audiences and US media in general.
Limitations: It is beyond the scope of this study to fully address the impact of the rise of streaming services on the cinema box office or the market for non-English-language films in the US. The planned world premiere of the director’s first English-language feature film in the fall of 2024 also represents a new direction for the filmmaker worthy of its own future study.
Contribution: The article takes a unique industry-focused approach to analysing both Almodóvar’s success and the US market and audience. It builds on current academic research and opens other clear avenues for further research. It can also provide strategic insights for producers, distributors and marketers.
Early and delayed STAT1-dependent responses drive local trained immunity of macrophages in the spleen
Authors: Solomon Aryeh , Bossel Ben-Moshe Noa , Hoffman Dotan , Trzebanski Sébastien , Yehezkel Dror , Vainman Leia , Netea Mihai G , Avraham Roi
Published: January 2025

Trained immunity (TI) is the process wherein innate immune cells gain functional memory upon exposure to specific ligands or pathogens, leading to augmented inflammatory responses and pathogen clearance upon secondary exposure. While the differentiation of hematopoietic stem cells (HSCs) and reprogramming of bone marrow (BM) progenitors are well-established mechanisms underpinning durable TI protection, remodeling of the cellular architecture within the tissue during TI remains underexplored. Here, we study the effects of peritoneal Bacillus Calmette–Guérin (BCG) administration to find TI-mediated protection in the spleen against a subsequent heterologous infection by the Gram-negative pathogen Salmonella Typhimurium (S.Tm). Utilizing single cell RNA-sequencing and flow cytometry, we discerned STAT1-regulated genes in TI-associated resident and recruited splenic myeloid populations. The temporal dynamics of TI were further elucidated, revealing both early and delayed myeloid subsets with time-dependent, cell-type-specific STAT1 signatures. Using lineage tracing, we find that tissue-resident red pulp macrophages (RPM), initially depleted by BCG exposure, are restored from both tissue-trained, self-renewing macrophages and from bone marrow-derived progenitors, fostering long lasting local defense. Early inhibition of STAT1 activation, using specific JAK-STAT inhibitors, reduces both RPM loss and recruitment of trained monocytes. Our study suggests a temporal window soon after BCG vaccination, in which STAT1-dependent activation of long-lived resident cells in the tissue mediates localized protection.
Exploration of the metabolomic mechanisms of postmenopausal hypertension induced by low estrogen state
Authors: Li Yao , Xin Hui , Lian Zhexun , Zhang Wei
Published: January 2025

Estrogen significantly impacts women’s health, and postmenopausal hypertension is a common issue characterized by blood pressure fluctuations. Current control strategies for this condition are limited in efficacy, necessitating further research into the underlying mechanisms. Although metabolomics has been applied to study various diseases, its use in understanding postmenopausal hypertension is scarce. Therefore, an ovariectomized rat model was used to simulate postmenopausal conditions. Estrogen levels, blood pressure, and aortic tissue metabolomics were analyzed. Animal models were divided into Sham, OVX, and OVX +E groups. Serum estrogen levels, blood pressure measurements, and aortic tissue metabolomics analyses were performed using radioimmunoassay, UHPLC-Q-TOF, and bioinformatics techniques. Based on the above research content, we successfully established a correlation between low estrogen levels and postmenopausal hypertension in rats. Notable differences in blood pressure parameters and aortic tissue metabolites were observed across the experimental groups. Specifically, metabolites that were differentially expressed, particularly L-alpha-aminobutyric acid (L-AABA), showed potential as a biomarker for postmenopausal hypertension, potentially exerting a protective function through macrophage activation and vascular remodeling. Enrichment analysis revealed alterations in sugar metabolism pathways, such as the Warburg effect and glycolysis, indicating their involvement in postmenopausal hypertension. Overall, this current research provides insights into the metabolic changes associated with postmenopausal hypertension, highlighting the role of L-AABA and sugar metabolism reprogramming in aortic tissue. The findings suggest a potential link between low estrogen levels, macrophage function, and vascular remodeling in the pathogenesis of postmenopausal hypertension. Further investigations are needed to validate these findings and explore their clinical implications for postmenopausal women.
Blocking SHP2 benefits FGFR2 inhibitor and overcomes its resistance in FGFR2-amplified gastric cancer
Authors: Zhang Yue , Wang Hanbing , Wei Yutao , Pan Yunfeng , Song Xueru , Shi Tao , Shao Jie , Yu Lixia , Liu Baorui , Wang Yue , Wei Jia
Published: January 2025

Fibroblast growth factor receptor 2 (FGFR2) is an important member of receptor tyrosine kinase (RTK) family. FGFR2 amplification occurs at a high frequency in gastric cancer (GC) and has been proven to be closely associated with poor prognosis and insensitivity to chemotherapy or immunotherapy. Current FGFR2-targeted therapies have limited efficacy. Hence, how to enhance efficacy and reverse resistance are urgent problems clinically. Src homology region 2-containing protein tyrosine phosphatase 2 (SHP2) serves as the shared downstream mediator of all RTKs and a prominence immunosuppressive molecule. In this study, we identified FGFR2 amplification in 6.2% (10/161) of GC samples in our center. Then we showed that dual blocking SHP2 and FGFR2 enhanced the effects of FGFR2 inhibitor (FGFR2i) in FGFR2-amplified GC both
in vitro
and
in vivo
via suppressing RAS/ERK and PI3K/AKT pathways. We further showed that it overcame FGFR2i resistance by reversing the feedback activation mediated by other RTKs and continuously suppressing FGFR2-initiated downstream pathways. Notably, SHP2 blockade could suppress PD-1 expression and promoted IFN-γ secretion of CD8
+
T cells, enhancing the cytotoxic functions of T cells in tumor immune microenvironment. Overall, our findings suggest that dual blocking SHP2 and FGFR2 is a compelling rationale with both targeted treatment and immune regulation for FGFR2-amplified GC.
Membrane mimetic thermal proteome profiling (MM-TPP) towards mapping membrane protein-ligand dynamics
Authors: Jandu Rupinder Singh , Al-Seragi Mohammed , Aoki Hiroyuki , Babu Mohan , van Hoa Franck Duong
Published: January 2025

Integral membrane proteins (IMPs) remain the principal target of small-molecule therapeutics, and yet modalities towards probing on and off-target hits against this protein class in a robust, unbiased, and detergent-free manner remain starkly underdeveloped. Previously, we introduced the Peptidisc membrane mimetic (MM) for the water-soluble stabilization of the
Escherichia coli
membrane proteome and interactome (Carlson et al., 2019). Herein, we implement the Peptidisc into thermal proteome profiling (TPP), enabling for the first time a broad-scale level characterization of membrane protein-ligand interactions while completely circumventing structural perturbations invoked by detergents. Using a library prepared from the whole mouse liver, we determine the influence of ATP and orthovanadate on the thermal stability of IMPs, including pharmaceutically relevant ATP-binding cassette ABC transporters and G-protein coupled receptors. MM-TPP also detects thermal stability changes driven by ATP by-products, where non-canonical ATP binders can be validated with next-generation computational tools. MM-TPP thus offers a robust platform for identifying on- and off-target ligand effects, providing insights into the druggable membrane proteome and its stability as a consequence of changing and often dynamic small molecules.
Twelve phosphomimetic mutations induce the assembly of recombinant full-length human tau into paired helical filaments
Authors: Lövestam Sofia , Wagstaff Jane L , Katsinelos Taxiarchis , Freund Stefan MV , Goedert Michel , Scheres Sjors HW
Published: January 2025

The assembly of tau into amyloid filaments is associated with more than twenty neurodegenerative diseases, collectively termed tauopathies. Cryo-EM structures of brain-derived tau filaments revealed that specific structures define different diseases, triggering a quest for the development of experimental model systems that replicate the structures of disease. Here, we describe twelve phosphomimetic, serine/threonine to aspartate, mutations in tau, which we termed PAD12, that collectively induce the
in vitro
assembly of full-length tau into filaments with the same structure as that of paired helical filaments extracted from the brains of individuals with Alzheimer’s disease. Solution-state nuclear magnetic resonance spectroscopy suggests that phosphomimetic mutations in the carboxy-terminal domain of tau may facilitate filament formation by disrupting an intramolecular interaction between two IVYK motifs. PAD12 tau can be used for both nucleation-dependent and multiple rounds of seeded assembly
in vitro
, as well as for the seeding of tau biosensor cells. PAD12 tau can be assembled into paired helical filaments under various shaking conditions, with the resulting filaments being stable for extended periods of time. They can be labelled with fluorophores and biotin. Tau filaments extracted from the brains of individuals with Alzheimer’s disease brains have been known to be made of hyperphosphorylated and abnormally phosphorylated full-length tau, but it was not known if the presence of this post-translational modification is more than a mere correlation. Our findings suggest that hyperphosphorylation of tau may be sufficient for the formation of the Alzheimer tau fold. PAD12 tau will be a useful tool for the study of molecular mechanisms of neurodegeneration.
Secretory leukocyte protease inhibitor influences periarticular joint inflammation in B. burgdorferi-infected mice
Authors: Yu Qian , Tang Xiaotian , Hart Thomas , Homer Robert , Belperron Alexia A , Bockenstedt Linda K , Ring Aaron , Nakamura Akira , Fikrig Erol
Published: January 2025

Lyme disease, caused by
Borrelia burgdorferi
, is the most common tick-borne infection in the United States. Arthritis is a major clinical manifestation of infection, and synovial tissue damage has been attributed to the excessive pro-inflammatory responses. The secretory leukocyte protease inhibitor (SLPI) promotes tissue repair and exerts anti-inflammatory effects. The role of SLPI in the development of Lyme arthritis in C57BL/6 mice, which can be infected with
B. burgdorferi
, but only develop mild joint inflammation, was therefore examined.
SLPI
-deficient C57BL/6 mice challenged with
B. burgdorferi
had a higher infection load in the tibiotarsal joints and marked periarticular swelling, compared to infected wild type control mice. The ankle joint tissues of
B. burgdorferi-
infected
SLPI
-deficient mice contained significantly higher percentages of infiltrating neutrophils and macrophages.
B. burgdorferi
-infected
SLPI
-deficient mice also exhibited elevated serum levels of IL-6, neutrophil elastase, and MMP-8. Moreover, using a recently developed BASEHIT (
BA
cterial
S
election to
E
lucidate
H
ost-microbe
I
nteractions in high
T
hroughput) library, we found that SLPI directly interacts with
B. burgdorferi
. These data demonstrate the importance of SLPI in suppressing periarticular joint inflammation in Lyme disease.
Study on the absorption characteristics of euscaphic acid and tiliroside in fruits of Rosa laxa Retz.
Authors: Wang Ning , Tian Li
Published: January 2025

The fruits of Rosa laxa Retz. (FRL) have a long history of medicinal use, known for their rich composition of flavonoids, polyphenols, amino acids, sugars, and other bioactive compounds. FRL exhibits pharmacological effects such as antioxidant, antiviral, antibacterial, and antitumor activities, making it a valuable resource with significant development potential in both the food and pharmaceutical industries. This study employed a response surface methodology combined with ultra-high-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-TQ-MS) to optimize FRL extraction. Reflux extraction was determined to be the most effective method with the following optimized parameters: 65% ethanol extraction solvent, material-to-liquid ratio of 1:35 (g/mL), and extraction time of 140 min, resulting in the FRL extract (FRLE). Under these optimized conditions, the extracted amount was extract was 51.00 ± 1.07%, the average content of total polyphenols was 126.55 ± 2.61 mg/g, and the average content of euscaphic acid was 2.90 ± 0.08 mg/g, demonstrating the efficiency of the extraction method. Using the Caco-2 cell model, the study investigated the absorption characteristics of euscaphic acid and tiliroside within FRLE. Results indicated that with increasing time, the absorbed amount (Qr) of euscaphic acid and tiliroside gradually increased, with an efflux ratio (RB→A/A→B) of less than 1.5, suggesting bidirectional drug transport with no significant directionality. Upon the addition of P-glycoprotein (P-gp) inhibitors Verapamil (Ver) and Ciclosporin A (CsA), as well as the chelating agent ethylenebis (oxyethylenenitrilo) tetraacetic acid (EGTA), Qr and Papp values notably increased, indicating that these two components are P-gp substrates with cellular basolateral efflux transport. Additionally, optimal absorption efficiency was observed under weakly acidic conditions (pH 6.0). In conclusion, euscaphic acid and tiliroside in FRLE demonstrated good membrane permeability, primarily relying on passive diffusion for absorption. This study offers experimental insights into the intestinal absorption of FRL in vivo.
Genetic Polymorphisms in RNF138, ABCA1 and ESRRG-GPATCH2 Genes and their Role in Insulin Resistance Risk among Normal BMI Individuals in Indian Population: A Case-control Study
Authors: Thummala Sabitha , Fathima Sarah , Kruthi Nithya , Ghori Junaid Ahmed Khan , Saikia Katherine , Belde Vivek , Rajendran Balamurali Andiyakkal , Ranganathan Rahul
Published: January 2025

Introduction: India, characterised as the “diabetes capital” of the world, faces a rapidly increasing diabetes crisis, with over 65 million cases diagnosed. Despite the growing prevalence, the genetic underpinnings of Insulin Resistance (IR) among Indians with a normal Body Mass Index (BMI) remains understudied.

Aim: To fill the knowledge gap by investigating the association of specific gene variants (RNF138, ABCA1, and Oestrogen-Related Receptor γ (ESRRG)-GPATCH2) with IR risk in this demographic.

Materials and Methods: This study was a case-control study conducted in Bengaluru, Karnataka, India on a total of 191 participants (90 men and 101 women). The study, including data collection and analysis, was completed over a period of six months. Participants were categorised into cases (Homeostasis Model Assessment Insulin Resistance (HOMA2- IR) >2) and controls (HOMA2-IR <2) based on HOMA2-IR values. Genotyping for rs4799327 (RNF138), rs2275543 (ABCA1), and rs1497828 (ESRRG-GPATCH2) was performed using the Illumina Infinium Global Screening Array (GSA). Statistical analyses, including Odds Ratios (ORs), 95% Confidence Intervals (CIs), and inheritance model analysis, were conducted to assess the association between genotypes and IR.

Results: Significant associations were found between IR and genetic variants rs4799327 (OR=2.74, 95% CI: 1.28-5.88, p-value=0.006) in RNF138, rs1497828 (OR=2.90, 95% CI: 1.51-5.57, p-value=0.0011) in ESRRG-GPATCH2 (dominant inheritance model), and rs2275543 (OR=3.50, 95% CI: 1.17- 10.42, p-value=0.011) in ABCA1 (additive model). The study highlights a notable susceptibility to IR linked to these genetic markers among individuals with a normal BMI in the Indian population.

Conclusion: This study underscores the importance of genetic factors in the risk of developing IR among Indians with a normal BMI, suggesting a complex interplay of genetics beyond traditional risk factors. These findings necessitate further research into the functional significance of these associations and their potential implications for targeted interventions and preventive strategies in high-risk populations.
Strategic Leadership in Multigenerational Workforces: Bridging Generational Divides for Enhanced Engagement
Authors: Nyamboga Tom Ongesa
Published: January 2025

This review examines the management of a multigenerational workforce, focusing on Baby Boomers, Generation X, Millennials, and Generation Z, and is underpinned by Upper Echelons Theory, which highlights how leaders' experiences and values influence organizational outcomes. The study explores strategies like customized communication, mentorship, flexible work options, and tailored development programs, with the aim of enhancing engagement, collaboration, and productivity across generational lines. Using a qualitative approach, the review synthesizes findings from recent empirical studies and reports to identify key challenges and opportunities for managing a diverse workforce. Among the key challenges include different communication preferences across generations and varying work values and expectations. The conclusion emphasizes the need for adaptable leadership that recognizes and addresses the distinct values and expectations of each generation. Its significance lies in offering actionable recommendations for organizations and policymakers to create inclusive environments that cater to generational diversity, fostering employee engagement, satisfaction, and retention. The review also advocates for investment in mentorship programs, flexible work arrangements, and personalized career development, while urging policymakers to implement supportive policies that enhance generational collaboration and contribute to long-term organizational success.
Comprehensive Sex Education for Youth Living with Disability in Botswana: Parents/Guardian Perspective in Serowe, Botswana
Authors: Phirinyane K. M. L , Maripe K. , Ntseane D.
Published: January 2025

This qualitative study explored the need for comprehensive sex education for youth with disability which heightens their vulnerability to sexual violence. The study sought to establish the existence of comprehensive sex education for youth with disability; describe the challenges of parents/guardians with the provision of such sex education; and identify strategies for reducing the challenges faced by parents/guardians. used the purposive sampling design to collect data. Data was collected through face-to-face interviews. The findings established that participants view sex education for youth with disability to be crucial. But parents/guardians must manage communication barriers; low comprehension level; distorted information; youth reciting sexual related information in public; and external influences. Therefore, the following interventions are necessary: comprehensive sex education for parents offered through seminars and workshops for distinct groups; and promote parent and stakeholder collaboration.
First Report of Dioscorea Bacilliform Virus, a Badnavirus Infecting Yam in Burkina Faso
Authors: Tapsoba Hermann , Ouattara Bakary , Koala Moustapha , Kaboré Emmanuel , Bonkoungou Yacouba , Dakouo Thyérry Kiébè , Sérémé Drissa
Published: January 2025

In Burkina Faso, yam plays an important role in food security and improving people's incomes. However, the sector is struggling to develop due to a number of constraints, including significant viral pressure. The Dioscorea bacilliform virus (DBV), prevalent in yam-growing areas of the West African region, is causing huge production losses. A survey followed by sample collection was carried out in yam fields in the Centre-Ouest and Cascades regions. A total of 32 samples were collected, of which 26 were symptomatic and 6 asymptomatic. The samples collected were subjected to biological, serological and molecular diagnosis. The study revealed the presence of DBV in all symptomatic samples tested. No asymptomatic samples were positive. Comparison of the sequences of the reverse transcriptase/ribonuclease H (RT/RNase H) region of the samples collected with those in the GenBank database confirmed the presence of DBV, with similarity rates ranging from 97.73% to 99.02%. Our work has thus made it possible to describe for the first time a new virus infecting yam in Burkina Faso. Studies are now required to generate epidemiological data on the virus and to search for resistance genes through varietal screening.