bluuebunny/crossref_metadata_2025 huggingface dataset:
Identifying genes related to choriogenesis in insect panoistic ovaries by Suppression Subtractive Hybridization
Authors: Irles Paula , Bellés Xavier , Piulachs M Dolors
Published: April 2009

Background

Insect ovarioles are classified into two categories: panoistic and meroistic, the later having apparently evolved from an ancestral panoistic type. Molecular data on oogenesis is practically restricted to meroistic ovaries. If we aim at studying the evolutionary transition from panoistic to meroistic, data on panoistic ovaries should be gathered. To this end, we planned the construction of a Suppression Subtractive Hybridization (SSH) library to identify genes involved in panoistic choriogenesis, using the cockroach Blattella germanica as model.

Results

We constructed a post-vitellogenic ovary library by SSH to isolate genes involved in choriogenesis in B. germanica. The tester library was prepared with an ovary pool from 6- to 7-day-old females, whereas the driver library was prepared with an ovary pool from 3- to 4-day-old females. From the SSH library, we obtained 258 high quality sequences which clustered into 34 unique sequences grouped in 19 contigs and 15 singlets. The sequences were compared against non-redundant NCBI databases using BLAST. We found that 44% of the unique sequences had homologous sequences in known genes of other organisms, whereas 56% had no significant similarity to any of the databases entries. A Gene Ontology analysis was carried out, classifying the 34 sequences into different functional categories. Seven of these gene sequences, representative of different categories and processes, were chosen to perform expression studies during the first gonadotrophic cycle by real-time PCR. Results showed that they were mainly expressed during post-vitellogenesis, which validates the SSH technique. In two of them corresponding to novel genes, we demonstrated that they are specifically expressed in the cytoplasm of follicular cells in basal oocytes at the time of choriogenesis.

Conclusion

The SSH approach has proven to be useful in identifying ovarian genes expressed after vitellogenesis in B. germanica. For most of the genes, functions related to choriogenesis are postulated. The relatively high percentage of novel genes obtained and the practical absence of chorion genes typical of meroistic ovaries suggest that mechanisms regulating chorion formation in panoistic ovaries are significantly different from those of meroistic ones.
Evaluation of the effect of stent strut profile on shear stress distribution using statistical moments
Authors: Mejia Juan , Ruzzeh Bilal , Mongrain Rosaire , Leask Richard , Bertrand Olivier F
Published: April 2009

Background

In-stent restenosis rates have been closely linked to the wall shear stress distribution within a stented arterial segment, which in turn is a function of stent design. Unfortunately, evaluation of hemodynamic performance can only be evaluated with long term clinical trials. In this work we introduce a set of metrics, based on statistical moments, that can be used to evaluate the hemodynamic performance of a stent in a standardized way. They are presented in the context of a 2D flow study, which analyzes the impact of different strut profiles on the wall shear stress distribution for stented coronary arteries.

Results

It was shown that the proposed metrics have the ability to evaluate hemodynamic performance quantitatively and compare it to a common standard. In the context of the simulations presented here, they show that stent's strut profile significantly affect the shear stress distribution along the arterial wall. They also demonstrates that more streamlined profiles exhibit better hemodynamic performance than the standard square and circular profiles. The proposed metrics can be used to compare results from different research groups, and provide an improved method of quantifying hemodynamic performance in comparison to traditional techniques.

Conclusion

The strut shape found in the latest generations of stents are commonly dictated by manufacturing limitations. This research shows, however, that strut design can play a fundamental role in the improvement of the hemodynamic performance of stents. Present results show that up to 96% of the area between struts is exposed to wall shear stress levels above the critical value for the onset of restenosis when a tear-drop strut profile is used, while the analogous value for a square profile is 19.4%. The conclusions drawn from the non-dimensional metrics introduced in this work show good agreement with an ordinary analysis of the wall shear stress distribution based on the overall area exposed to critically low wall shear stress levels. The proposed metrics are able to predict, as expected, that more streamlined profiles perform better hemodynamically. These metrics integrate the entire morphology of the shear stress distribution and as a result are more robust than the traditional approach, which only compares the relative value of the local wall shear stress with a critical value of 0.5 Pa. In the future, these metrics could be employed to compare, in a standardized way, the hemodynamic performance of different stent designs.
A rapid method of fruit cell isolation for cell size and shape measurements
Authors: McAtee Peter A , Hallett Ian C , Johnston Jason W , Schaffer Robert J
Published: April 2009

Background

Cell size is a structural component of fleshy fruit, contributing to important traits such as fruit size and texture. There are currently a number of methods for measuring cell size; most rely either on tissue sectioning or digestion of the tissue with cell wall degrading enzymes or chemicals to release single cells. Neither of these approaches is ideal for assaying large fruit numbers as both require a considerable time to prepare the tissue, with current methods of cell wall digestions taking 24 to 48 hours. Additionally, sectioning can lead to a measurement of a plane that does not represent the widest point of the cell.

Results

To develop a more rapid way of measuring fruit cell size we have developed a protocol that solubilises pectin in the middle lamella of the plant cell wall releasing single cells into a buffered solution. Gently boiling small fruit samples in a 0.05 M Na2CO3solution, osmotically balanced with 0.3 M mannitol, produced good cell separation with little cellular damage in less than 30 minutes. The advantage of combining a chemical treatment with boiling is that the cells are rapidly killed. This stopped cell shape changes that could potentially occur during separation. With this method both the rounded and angular cells of the apple cultivars SciRos 'Pacific Rose' and SciFresh 'Jazz'™ were observed in the separated cells. Using this technique, an in-depth analysis was performed measuring cell size from 5 different apple cultivars. Cell size was measured using the public domain ImageJ software. For each cultivar a minimum of 1000 cells were measured and it was found that each cultivar displayed a different distribution of cell size. Cell size within cultivars was similar and there was no correlation between flesh firmness and cell size. This protocol was tested on tissue from other fleshy fruit including tomato, rock melon and kiwifruit. It was found that good cell separation was achieved with flesh tissue from all these fruit types, showing a broad utility to this protocol.

Conclusion

We have developed a method for isolating single cells from fleshy fruit that reduces the time needed for fruit cell separation. This method was used to demonstrate differences in cell size and shape for 5 different apple cultivars. While firmness between the different cultivars is independent of cell size, apples with more angular cells appear to be firmer.
Social influences upon injection initiation among street-involved youth in Vancouver, Canada: a qualitative study
Authors: Small Will , Fast Danya , Krusi Andrea , Wood Evan , Kerr Thomas
Published: April 2009

Background

Street-involved youth are a population at risk of adopting injection as a route of administration, and preventing the transition to injection drug use among street youth represents a public health priority. In order to inform epidemiological research and prevention efforts, we conducted a qualitative study to investigate the initiation of injection drug use among street-involved youth in Vancouver, Canada.

Methods

Qualitative interviews with street youth who inject drugs elicited descriptions of the adoption of injection as a route of administration. Interviewees were recruited from the At-Risk Youth Study (ARYS), a cohort of street-involved youth who use illicit drugs in Vancouver, Canada. Audio recorded interviews were transcribed verbatim and a thematic analysis was conducted.

Results

26 youth aged 16 to 26 participated in this study, including 12 females. Among study participants the first injection episode frequently featured another drug user who facilitated the initiation of injecting. Youth narratives indicate that the transition into injecting is influenced by social interactions with drug using peers and evolving perceptions of injecting, and rejecting identification as an injector was important among youth who did not continue to inject. It appears that social conventions discouraging initiating young drug users into injection exist among established injectors, although this ethic is often ignored.

Conclusion

The importance of social relationships with other drug users within the adoption of injection drug use highlights the potential of social interventions to prevent injection initiation. Additionally, developing strategies to engage current injectors who are likely to initiate youth into injection could also benefit prevention efforts.
Further validation of the Health Scale of Traditional Chinese Medicine (HSTCM)
Authors: Wu Darong , Lai Shilong , Zhou Luojing , Guo Xinfeng , Liang Weixiong , Wen Zehuai , Ou Aihua , Zhang Guangqing , Chen Keji
Published: April 2009

Background

Few health measurement scales are based on Chinese medicine theory. The Health Scale of Traditional Chinese Medicine (HSTCM) was developed to fill this gap. The aim of this study is to validate the HSTCM.

Methods

A convenience sample of 630 participants was recruited in 11 settings. All participants were asked to complete the HSTCM and World Health Organization Quality of Life Measure-Abbreviated Version (WHOQOL-BREF).

Results

Properties of the HSTCM were tested. Intra-class correlation coefficient representing the inter-interviewer reliability was 0.99 (95%CI) for the overall instrument. Spearman-Brown correlation coefficient and Cronbach's coefficient alpha were 0.81 and 0.94 respectively, indicating satisfactory internal reliability and inter-interviewer reliability. Spearman's rho correlation coefficient between the HSTCM and WHOQOL-BREFF was -0.67. A receiver operating characteristic (ROC) curve analysis was performed to test the discriminate validation. Areas under the ROC curve analysis for the HSTCM and its domains ranged 0.71–0.87 and all the lower levels of 95%CI were greater than 0.50.

Conclusion

The HSTCM was validated as a generic health scale and may complement existing health measurement scales in Chinese medicine health care.
Tumour-associated tenascin-C isoforms promote breast cancer cell invasion and growth by matrix metalloproteinase-dependent and independent mechanisms
Authors: Hancox Rachael A , Allen Michael D , Holliday Deborah L , Edwards Dylan R , Pennington Caroline J , Guttery David S , Shaw Jacqueline A , Walker Rosemary A , Pringle J Howard , Jones J Louise
Published: April 2009

Introduction

The stromal microenvironment has a profound influence on tumour cell behaviour. In tumours, the extracellular matrix (ECM) composition differs from normal tissue and allows novel interactions to influence tumour cell function. The ECM protein tenascin-C (TNC) is frequently up-regulated in breast cancer and we have previously identified two novel isoforms – one containing exon 16 (TNC-16) and one containing exons 14 plus 16 (TNC-14/16).

Methods

The present study has analysed the functional significance of this altered TNC isoform profile in breast cancer. TNC-16 and TNC-14/16 splice variants were generated using PCR-ligation and over-expressed in breast cancer cells (MCF-7, T47D, MDA-MD-231, MDA-MB-468, GI101) and human fibroblasts. The effects of these variants on tumour cell invasion and proliferation were measured and compared with the effects of the large (TNC-L) and fully spliced small (TNC-S) isoforms.

Results

TNC-16 and TNC-14/16 significantly enhanced tumour cell proliferation (P < 0.05) and invasion, both directly (P < 0.01) and as a response to transfected fibroblast expression (P < 0.05) with this effect being dependent on tumour cell interaction with TNC, because TNC-blocking antibodies abrogated these responses. An analysis of 19 matrix metalloproteinases (MMPs) and tissue inhibitor of matrix metalloproteinases 1 to 4 (TIMP 1 to 4) revealed that TNC up-regulated expression of MMP-13 and TIMP-3 two to four fold relative to vector, and invasion was reduced in the presence of MMP inhibitor GM6001. However, this effect was not isoform-specific but was elicited equally by all TNC isoforms.

Conclusions

These results demonstrate a dual requirement for TNC and MMP in enhancing breast cancer cell invasion, and identify a significant role for the tumour-associated TNC-16 and TNC-14/16 in promoting tumour invasion, although these isoform-specific effects appear to be mediated through MMP-independent mechanisms.
Post-traumatic stress disorder and attachment: possible links with borderline personality disorder
Authors: de Zulueta Felicity
Published: April 2009

This article discusses the aetiology of both simple and complex post-traumatic stress disorders (PTSDs) in terms of attachment theory, and points out the similarities between the diagnosis of complex PTSD and of borderline personality disorder. Case vignettes illustrate an outline of the assessment and treatment of the psychobiological symptoms of PTSD informed by attachment research.
Minimal-medication approaches to treating schizophrenia
Authors: Calton Tim , Spandler Helen
Published: April 2009

UK guidelines for treating people diagnosed with schizophrenia currently emphasise the primacy of antipsychotic medication, with or without psychosocially based interventions as circumstances dictate. We now see increasing calls, most notably from mental health service users, for the provision of ‘whole-person-based’, minimal-medication approaches to treating people with this diagnosis. This article is intended to locate the development of such approaches within the history of modern and pre-modern psychiatry and, in doing so, summarise the available evidence base that underpins their efficacy.
Introducing compassion-focused therapy
Authors: Gilbert Paul
Published: April 2009

Shame and self-criticism are transdiagnostic problems. People who experience them may struggle to feel relieved, reassured or safe. Research suggests that a specialised affect regulation system (or systems) underpins feelings of reassurance, safeness and well-being. It is believed to have evolved with attachment systems and, in particular, the ability to register and respond with calming and a sense of well-being to being cared for. In compassion-focused therapy it is hypothesised that this affect regulation system is poorly accessible in people with high shame and self-criticism, in whom the ‘threat’ affect regulation system dominates orientation to their inner and outer worlds. Compassion-focused therapy is an integrated and multimodal approach that draws from evolutionary, social, developmental and Buddhist psychology, and neuroscience. One of its key concerns is to use compassionate mind training to help people develop and work with experiences of inner warmth, safeness and soothing, via compassion and self-compassion.
How to make good-enough risk decisions
Authors: Carroll Andrew
Published: April 2009

Making decisions in the context of risk is an integral part of psychiatric work. Despite this, decision-making skills are rarely systematically taught and the processes behind decisions are rarely made explicit. This article attempts to apply contemporary evidence from cognitive and social psychology to common dilemmas faced by psychiatrists when assessing and managing risk. It argues that clinical decision-making should acknowledge both the value and limitations of intuitive approaches in dealing with complex dilemmas. After discussing the various ways in which clinical decision-making is commonly derailed, the article outlines a framework that accommodates both rational and intuitive modes of thinking, with the aim of optimising decision-making in high-risk situations.
Drug treatments for attention-deficit hyperactivity disorder in young people
Authors: Bates Gordon
Published: April 2009

The central role of medication in the treatment of attention-deficit hyperactivity disorder (ADHD) in children and adolescents is the focus of controversy once more. Questions about the safety and appropriateness of medication have arisen both within and without the medical community. This coincides with the disappointing results for long-term ADHD treatment, illustrated recently by the first major outcome study to be published. Clinicians have had to contend with a number of public health scares following on from the actions of drug regulatory bodies, in addition to misinformation and misunderstanding from the media. Ironically, this comes at a time when we have an ever-widening range of medications and formulations available to us, and better-quality studies to inform treatment decisions. The recently published National Institute for Health and Clinical Excellence guidelines address some of these issues and provide a welcome summary and reference point for beleaguered clinicians.
Issues concerning feedback about genetic testing and risk of depression
Authors: Wilhelm Kay , Meiser Bettina , Mitchell Philip B. , Finch Adam W. , Siegel Jennifer E. , Parker Gordon , Schofield Peter R.
Published: April 2009

Background

Recent studies show that adverse life events have a significantly greater impact on depression onset for those with the s/s allele of the genotype for the 5-HT gene-linked promoter region. Research in genes related to risk of depression leads to the question of how this information is received by individuals.

Aims

To investigate factors related to the response to receiving one's own serotonin transporter genotype results.

Method

Predictors of the impact of receiving individual genotype data were assessed in 128 participants in a study of gene–environment interaction in depression onset.

Results

Two-thirds decided to learn their individual genotype results (receivers) and prior to disclosure this decision was associated with a perception of greater benefit from receipt of the information (P=0.001). Receivers completing the 2-week (n=76) and 3-month follow-up (n=78) generally reported feeling pleased with the information and having had a more positive experience than distress. However, distress was related to genotype, with those with the s/s allele being most affected.

Conclusions

There was high interest in, and satisfaction with, learning about one's serotonin transporter genotype. Participants appeared to understand that the gene conferred susceptibility to depression rather than a direct causal effect.
Psychosis and autism: magnetic resonance imaging study of brain anatomy
Authors: Toal Fiona , Bloemen Oswald J. N. , Deeley Quinton , Tunstall Nigel , Daly Eileen M. , Page Lisa , Brammer Michael J. , Murphy Kieran C. , Murphy Declan G. M.
Published: April 2009

Background

Autism-spectrum disorder is increasingly recognised, with recent studies estimating that 1% of children in South London are affected. However, the biology of comorbid mental health problems in people with autism-spectrum disorder is poorly understood.

Aims

To investigate the brain anatomy of people with autism-spectrum disorder with and without psychosis.

Method

We used in vivo magnetic resonance imaging and compared 30 adults with autism-spectrum disorder (14 with a history of psychosis) and 16 healthy controls.

Results

Compared with controls both autism-spectrum disorder groups had significantly less grey matter bilaterally in the temporal lobes and the cerebellum. In contrast, they had increased grey matter in striatal regions. However, those with psychosis also had a significant reduction in grey matter content of frontal and occipital regions. Contrary to our expectation, within autism-spectrum disorder, comparisons revealed that psychosis was associated with a reduction in grey matter of the right insular cortex and bilaterally in the cerebellum extending into the fusiform gyrus and the lingual gyrus.

Conclusions

The presence of neurodevelopmental abnormalities normally associated with autism-spectrum disorder might represent an alternative ‘entry-point’ into a final common pathway of psychosis.
Anterior cingulate cortex abnormalities associated with a first psychotic episode in bipolar disorder
Authors: Fornito Alex , Yücel Murat , Wood Stephen J. , Bechdolf Andreas , Carter Simon , Adamson Chris , Velakoulis Dennis , Saling Michael M. , McGorry Patrick D. , Pantelis Christos
Published: April 2009

Background

The anterior cingulate cortex is frequently implicated in the pathophysiology of bipolar disorder, but magnetic resonance imaging (MRI) studies have reported variable findings owing to a reliance on patient samples with chronic illness and to limited appreciation of the region's heterogeneity.

Aims

To characterise anterior cingulate cortex abnormalities in patients with bipolar disorder experiencing their first episode of psychosis while accounting for regional anatomical variability.

Method

Grey matter volume, surface area and cortical thickness were measured in six anterior cingulate cortex subregions per hemisphere using MRI scans acquired from 26 patients with bipolar I disorder experiencing first-episode psychosis and 26 healthy controls matched for age, gender and regional morphological variability.

Results

Relative to controls, male patients displayed increased thickness in the right subcallosal limbic anterior cingulate cortex. No significant differences were identified in females for grey matter volume or surface area measures. The findings were not attributable to medication effects.

Conclusions

These data suggest that first-episode psychosis in bipolar disorder is associated with a gender-specific, right-lateralised thickness increase in anterior cingulate cortex subregions known to play a role in regulating physiological stress responses.
Cognitive effectiveness of olanzapine and risperidone in first-episode psychosis
Authors: Cuesta Manuel J. , de Jalón Elena García , Campos M. Soledad , Peralta Victor
Published: April 2009

Background

Cognitive impairment in schizophrenia-spectrum disorders is highly prevalent and notably influences functional outcomes.

Aims

To characterise the cognitive effectiveness of second-generation antipsychotic drugs.

Method

One hundred consecutive and previously unmedicated patients with first-episode schizophrenia-spectrum disorders were admitted. Seventy-seven completed baseline, 1-month and 6-month psychopathological and neuropsychological assessments. Patients were randomised to risperidone or olanzapine treatment. Four final treatment allocation groups were defined since patients continued treatment in their normal setting: risperidone, olanzapine, mixed and no-antipsychotic groups.

Results

There were no differences in cognitive effectiveness between the four treatment groups. Reliable change index methods demonstrated that nearly a half of patients showed an improvement in Global Cognitive Score at the 6-month assessment. Improvement on the neuropsychological tests ranged from 17 to 54%.

A strong predictor of cognitive response was poor performance on baseline neuropsychological tests; response was moderately influenced by a low premorbid scholastic performance and IQ.

Conclusions

Cognitive improvement related to second-generation antipsychotic drugs appeared within the first 4 weeks of treatment and persisted at 6 months irrespective of treatment group. Greater cognitive dysfunction at baseline and lower premorbid cognitive background predicted cognitive improvement in our sample.
Lithium levels in drinking water and risk of suicide
Authors: Ohgami Hirochika , Terao Takeshi , Shiotsuki Ippei , Ishii Nobuyoshi , Iwata Noboru
Published: April 2009

Although lithium is known to prevent suicide in people with mood disorders, it is uncertain whether lithium in drinking water could also help lower the risk in the general population. To investigate this, we examined lithium levels in tap water in the 18 municipalities of Oita prefecture in Japan in relation to the suicide standardised mortality ratio (SMR) in each municipality. We found that lithium levels were significantly and negatively associated with SMR averages for 2002–2006. These findings suggest that even very low levels of lithium in drinking water may play a role in reducing suicide risk within the general population.
Accountability of specialist child and adolescent mental health services
Authors: Garralda Elena M.
Published: April 2009

Outcome auditing of specialist child and adolescent mental health services (CAMHS) is now well under way internationally. There is, however, debate about objectives and tools. A case is made for the achievable goal of enhancing service accountability through user satisfaction information and clinician-rated contextualised measures of improvements in symptoms and impairment.
Convulsive therapy turns 75
Authors: Gazdag Gabor , Bitter Istvan , Ungvari Gabor S. , Baran Brigitta
Published: April 2009

The Hungarian psychiatrist Laszlo Meduna was the first who induced epileptic fits to influence the course of mental illness. The following account, based on a review of Meduna's recently unearthed files and his writings, traces the beginnings of convulsive therapy.
Psychiatric training – a dangerous pursuit
Authors: Molyneux Guy , Wright Brenda , Rush Gavin , Reidy Julianne , Campbell Fiona , Ryan Maria , Leonard Peter , Ambikapthy Anita
Published: April 2009

We performed a review of the international literature, Royal College of Psychiatrists guidelines and Irish legislation concerning psychiatric trainees and their experience of violence. Physical violence in the workplace was reported by 16% of trainees in Ireland and 67% of specialist registrars in the UK; 72% of trainees in Belgium reported verbal violence. Personal characteristics of trainees which increase the risk of experiencing violence are under-researched, although it is observed that the duration of clinical experience seems to be somewhat protective. the advent of community psychiatry brings new risks to trainees. the Royal College of Psychiatrists issued guidelines and reports that are useful in developing facilities and promoting trainee safety. Although legislation provides some protection to trainees, it also places responsibility on them as employees.
First-episode psychosis: primary care experience and implications for service development
Authors: El-Adl Mamdouh , Burke John , Little Karen
Published: April 2009

Aim and Methods

To capture the local primary care experience of first-episode psychosis before developing a local early intervention in psychosis service. A survey of Northamptonshire general practitioners (GPs) using a confidential questionnaire was carried out.

Results

Out of 284 GPs, 123 (43%) responded. General practitioners are unlikely to start treatment before referring to a specialist service: 63 GPs (51% of responders) start treatment in 10% or less of individuals with first-episode psychosis and 19 (15.5%) GPs start treatment in 75% or more before referring them to psychiatric service; 42 GPs (34%) refer those who request/accept a referral and 66 GPs (53%) refer all even if they refuse. Overall, 92 GPs (74%) agreed that an early intervention in psychosis service is needed and 77 (63%) GPs welcome having a mental health clinic in their surgery.

Clinical Implications

Individuals are more likely to accept referral to a psychiatric service if offered than to ask for it. People disengaging, stigma, the service being difficult to access/inappropriate and carers' lack of knowledge about mental illness are the likely causes for delayed referral.
Ireland's Mental Health Act 2001: where are we now?
Authors: Mhaoláin Áine Ni , Kelly Brendan D.
Published: April 2009

Ireland's Mental Health Act 2001 was fully implemented in 2006 and aimed to bring Irish legislation more in line with international standards such as the European Convention on Human Rights and United Nations Principles for the Protection fo Persons with Mental Illness. the new legislation introduced several important reforms in relation to involuntary admission, independent reviews of involuntary detention, consent to treatment, and treatment of children and adolescents. Although the Mental Health Act 2001 focuses clearly on protecting the right to liberty, it also presents significant challenges in terms of service delivery and resources within Irish mental health services.
Working as a trainee in Australia: the essential steps before working out of programme
Authors: Sohal Ravi
Published: April 2009

This article outlines how trainees can work in Australia without disrupting their career plan and how this can be accredited to their clinical training. In particular, the processes involved in obtaining approval for a post from the Royal College of Psychiatrists, the London Deanery, and the Department of Immigration and Medical Registration in Australia are described in detail.
Using postal questionnaires to identify carer depression prior to initial patient contact
Authors: Graham Candida R. , Banerjee Sube , Gill Randeep S.
Published: April 2009

Aims and Method

To assess whether postal questionnaires, used as a local initiative, were useful in identifying carer depression allowing early support for community-dwelling carers of older adults with mental health needs. the Geriatric Depression Scale and a questionnaire collecting information on the carer's circumstances were sent to carers of consecutive patients routinely referred to a community mental health team for older adults in south London. Rates of carer depression between postal questionnaire responders and non-responders were compared.

Results

The response rate to the postal questionnaires (33%) was similar to that observed in other postal studies; 42% of responders had depression compared with only 4.6% of non-responders.

Clinical Implications

Pre-contact postal questionnaires may present a simple method of enhancing early detection of carer depression for minimal economic outlay.
Future of Cancer Incidence in the United States: Burdens Upon an Aging, Changing Nation
Authors: Smith Benjamin D. , Smith Grace L. , Hurria Arti , Hortobagyi Gabriel N. , Buchholz Thomas A.
Published: April 2009

Purpose

By 2030, the United States' population will increase to approximately 365 million, including 72 million older adults (age ≥ 65 years) and 157 million minority individuals. Although cancer incidence varies by age and race, the impact of demographic changes on cancer incidence has not been fully characterized. We sought to estimate the number of cancer patients diagnosed in the United States through 2030 by age and race.

Methods

Current demographic-specific cancer incidence rates were calculated using the Surveillance Epidemiology and End Results database. Population projections from the Census Bureau were used to project future cancer incidence through 2030.

Results

From 2010 to 2030, the total projected cancer incidence will increase by approximately 45%, from 1.6 million in 2010 to 2.3 million in 2030. This increase is driven by cancer diagnosed in older adults and minorities. A 67% increase in cancer incidence is anticipated for older adults, compared with an 11% increase for younger adults. A 99% increase is anticipated for minorities, compared with a 31% increase for whites. From 2010 to 2030, the percentage of all cancers diagnosed in older adults will increase from 61% to 70%, and the percentage of all cancers diagnosed in minorities will increase from 21% to 28%.

Conclusion

Demographic changes in the United States will result in a marked increase in the number of cancer diagnoses over the next 20 years. Continued efforts are needed to improve cancer care for older adults and minorities.
American Society of Clinical Oncology Policy Statement: Disparities in Cancer Care
Authors: Goss Elizabeth , Lopez Ana Maria , Brown Carol L. , Wollins Dana S. , Brawley Otis W. , Raghavan Derek
Published: April 2009

The American Society of Clinical Oncology (ASCO) has embarked on an intensive campaign to integrate elimination of cancer health disparities into the Society's overall mission and activities. Key components of this commitment are enhancing awareness of disparities; improving access to care; and supporting research on health disparities. Major objectives are to advance the education of the oncology community in the care of patients from underserved and minority populations; increase the diversity of the clinical oncology workforce as a requisite to improving access to cancer care for the underserved; and support research in the area of health disparities. Racial and ethnic disparities in cancer care are an issue of critical importance to ASCO, the oncology community, and our society at large. The health disparities initiative outlined herein enunciates ASCO's dedication to eliminating disparities in cancer care and discusses our multipronged approach for addressing disparities within the clinical oncology community. ASCO is committed to collaborating with the diverse community of stakeholders to undertake the following: develop policies to guarantee equal access to quality health care, with special emphasis on reducing insurance and economic barriers to cancer care; develop a comprehensive plan to increase awareness of racial and ethnic disparities in cancer care; execute a strategy to enhance the supply of minority physicians and to improve the training of the oncology workforce to meet the needs of racially and ethnically diverse cancer patients; increase prioritization of public and private research on cancer care disparities; develop mechanisms to increase participation of racially and ethnically diverse populations in cancer clinical trials; and support initiatives to enhance patients' involvement in their cancer care.
The Ideology of the Mermaid
Authors: Matthew Patricia A. , Greenberg Jonathan
Published: April 2009

This article argues that introducing undergraduates to literary criticism and theory can be most effectively accomplished through the teaching of children's literature, fantasy literature, and Disney films alongside traditional literary criticism. We discuss a series of assignments we use in Pursuits of English, our department's introductory theory and criticism course.
From Language Experience to Classroom Practice
Authors: Lovejoy Kim Brian , Fox Steve , Wills Katherine V.
Published: April 2009

This article describes specific language experiences of three college writing teachers and the classroom practices that have resulted from these experiences. The authors want to raise awareness of linguistic diversity in writing classes and to help teachers connect with their own language experiences in order to integrate policies and practices that value students' own language varieties.
The Class as Periodical
Authors: Lund Michael , McReynolds Leigha
Published: April 2009

Attention to the similarities between an academic class and a magazine illuminates how periodicity affects the reading and learning experience. Focusing on the subscribers' power in shaping the continuing life of a periodical, the teaching methodology presented here also underscores the collaborative nature of all teaching.
What Looms
Authors: Jackson Chuck
Published: April 2009

This article explores how the author folds prison studies into his composition courses at Texas' only open-admissions university, located directly across from a massive county jail bearing an uncanny resemblance to his home institution. The author not only examines the semiotics of the two buildings but also explains how and why he teaches students about the jail and its connection to a larger system of punishment. Asking first-year students to research a accustomed part of their local surroundings demystifies their understanding of incarceration as it helps to demystify the entire experience of research, writing, and going to school in a unique urban setting. Such a move fosters for the students a theoretical and experiential connection between public education and critical citizenship. It also reminds students to take a good look around (no matter where they are) and think more deeply about what's there, what's not there, and why.
Beyond Critical Thinking
Authors: Clark Miriam Marty
Published: April 2009

Critical thinking skills are valued across the university. Derek Bok writes that 90 percent of faculty identify critical thinking as the most important goal of a university education. In English and foreign language departments, critical thinking has often served as a default goal when faculty cannot agree on which texts or approaches to teach. Without disputing the importance of these skills, I argue that an exclusive focus on critical thinking compromises more modest but also very worthy aims, including appreciation. This article makes the case for renewed attention to appreciation as a goal of literary study. I argue that teaching appreciation helps to cultivate virtues of open-mindedness, responsiveness, and attunement, and that such teaching may be useful in addressing widespread declines in reading and reading skills. At the end of the essay I describe changes I have made in my own teaching practices to emphasize literary appreciation.
Barbarians at the Gate
Authors: Hanstedt Paul , Hollis Gary , Ankney Rachelle , Zorn Hans , Marsh Gordon
Published: April 2009

The Roanoke College Writing Initiative Grant (WIG) program provides a two-thousand-dollar stipend for non-English Department faculty to teach in the first-year writing program. Faculty is expected to teach three iterations of their proposed course and receive a year of training prior to entering the classroom. Hanstedt's introduction discusses the theoretical justifications for the program, as well as its historical roots and positive outcomes.

The faculty development training of Roanoke's WIG program is described, as is how this member of the chemistry department put the lessons learned into action as he taught freshman writing for the first time.

Rachelle Ankney taught an introductory writing course as a break from teaching many sections of introductory college math. She enjoyed learning a whole new approach to writing and had fun in the first-year writing course. But she was most surprised to find that teaching writing well makes teaching math better, too. She went from advocating “required writing across the curriculum” to being a firm supporter of “teaching writing across the curriculum.”

This paper reflects on an experiment in using a writing course to teach critical thinking skills and vice versa, with special emphasis on helping students to get beyond their aversion to and distrust of argument. The course assigned short argument analyses, an exercise in literary interpretation, and a research paper in for students to gain more familiarity with argument and to appreciate its varied uses. One unforeseen result was the amount of time that had to be devoted to clarification of the terms of argument. Because clarification requires using inference, however, it is recommended that descriptive writing would be a helpful vehicle to start students addresstheir problems involving argument.

This paper recounts a music professor's experience designing and teaching his first writing course, Music into Words. Research on the conceptualization of music argues that our ability to communicate musical understanding relies heavily on phenomenological and metaphorical description; the opportunity to teach writing about music to the general student offered the musician a laboratory for testing this hypothesis. However, the instructor discovered that, not surprisingly, narrative (story-telling) functioned as his students' primary mode of communicating meaning and significance in music. In the end, while reading and writing these stories, the students and the music professor learn important lessons about the role of music in human experience.
Neuropilin receptors guide distinct phases of sensory and motor neuronal segmentation
Authors: Roffers-Agarwal Julaine , Gammill Laura S.
Published: April 2009

The segmented trunk peripheral nervous system is generated by ventrally migrating neural crest cells that exclusively invade the anterior sclerotome and differentiate into metameric dorsal root and sympathetic ganglia. Meanwhile, ventral spinal motor axons also project through the somites in a segmental fashion. How peripheral nervous system segmentation is generated is unknown. We previously showed that neuropilin 2 (Nrp2)/semaphorin 3F (Sema3F)signaling is required for segmental neural crest migration, but not for metameric dorsal root gangliogenesis. We now expand these results to show that Nrp2 patterns initial motor axon outgrowth as well. Later, Nrp1/Sema3A signaling is essential for segmental dorsal root gangliogenesis and motor axonal fasciculation into ventral roots. Strikingly, Nrp/Sema signaling is not required for sympathetic ganglia segmentation. These data show that Nrp2 and Nrp1 work together to produce segmentation of sensory and motor nerves, and that dorsal peripheral nervous system metamerism is generated in a stepwise,Nrp-dependent process.
Brca1 is required for embryonic development of the mouse cerebral cortex to normal size by preventing apoptosis of early neural progenitors
Authors: Pulvers Jeremy N. , Huttner Wieland B.
Published: April 2009

The extent of apoptosis of neural progenitors is known to influence the size of the cerebral cortex. Mouse embryos lacking Brca1, the ortholog of the human breast cancer susceptibility gene BRCA1, show apoptosis in the neural tube, but the consequences of this for brain development have not been studied. Here we investigated the role of Brca1 during mouse embryonic cortical development by deleting floxed Brca1 using Emx1-Cre, which leads to conditional gene ablation specifically in the dorsal telencephalon after embryonic day (E) 9.5. The postnatal Brca1-ablated cerebral cortex was substantially reduced in size with regard to both cortical thickness and surface area. Remarkably,although the thickness of the cortical layers (except for the upper-most layer) was decreased, cortical layering as such was essentially unperturbed. High levels of apoptosis were found at E11.5 and E13.5, but dropped to near-control levels by E16.5. The apoptosis at the early stage of neurogenesis occurred in both BrdU pulse-labeled neural progenitors and the neurons derived therefrom. No changes were observed in the mitotic index of apical(neuroepithelial, radial glial) progenitors and basal (intermediate)progenitors, indicating that Brca1 ablation did not affect cell cycle progression. Brca1 ablation did, however, result in the nuclear translocation of p53 in neural progenitors, suggesting that their apoptosis involved activation of the p53 pathway. Our results show that Brca1is required for the cerebral cortex to develop to normal size by preventing the apoptosis of early cortical progenitors and their immediate progeny.
Multiple roles for Sox2 in the developing and adult mouse trachea
Authors: Que Jianwen , Luo Xiaoyan , Schwartz Robert J. , Hogan Brigid L. M.
Published: April 2009

The esophagus, trachea and lung develop from the embryonic foregut, yet acquire and maintain distinct tissue phenotypes. Previously, we demonstrated that the transcription factor Sox2 is necessary for foregut morphogenesis and esophagus development. We show that Sox2 is also required for the normal development of the trachea and lung. In both the embryo and adult, Sox2 is exclusively expressed in the epithelium of the trachea and airways. We use an Nkx2.5-Cre transgene and a Sox2 floxed allele to conditionally delete Sox2 in the ventral epithelial domain of the early anterior foregut, which gives rise to the future trachea and lung buds. All conditional mutants die of respiratory distress at birth, probably due to abnormal differentiation of the laryngeal and tracheal cartilage as a result of defective epithelial-mesenchymal interaction. About 60% of the mutants have a short trachea, suggesting that the primary budding site of the lung shifts anteriorly. In the tracheal epithelium of all conditional mutants there are significantly more mucus-producing cells compared with wild type, and fewer basal stem cells, ciliated and Clara cells. Differentiation of the epithelium lining the conducting airways in the lung is abnormal, suggesting that Sox2 also plays a role in the differentiation of embryonic airway progenitors into specific lineages. Conditional deletion of Sox2 was then used to test its role in adult epithelium maintenance. We found that epithelial cells,including basal stem cells, lacking Sox2 show a reduced capacity to proliferate in culture and to repair after injury in vivo. Taken together,these results define multiple roles for Sox2 in the developing and adult trachea.
A comprehensive definition for metabolic syndrome
Authors: Huang Paul L.
Published: April 2009

The metabolic syndrome refers to the co-occurrence of several known cardiovascular risk factors, including insulin resistance, obesity, atherogenic dyslipidemia and hypertension. These conditions are interrelated and share underlying mediators, mechanisms and pathways. There has been recent controversy about its definition and its utility. In this article, I review the current definitions for the metabolic syndrome and why the concept is important. It identifies a subgroup of patients with shared pathophysiology who are at high risk of developing cardiovascular disease and type 2 diabetes. By considering the central features of the metabolic syndrome and how they are related, we may better understand the underlying pathophysiology and disease pathogenesis. A comprehensive definition for the metabolic syndrome and its key features would facilitate research into its causes and hopefully lead to new insights into pharmacologic and lifestyle treatment approaches.
Using zebrafish to understand the genome: an interview with Nancy Hopkins
Authors: Kain Kristin
Published: April 2009

Nancy Hopkins is famous for pushing the envelope: both in her pursuit to understand cancer genetics and in exposing professional disparities between men and women. Here, she discusses influential moments that have defined her career path, and her concerns for the future.