bluuebunny/crossref_metadata_2025 huggingface dataset:
The Use of Natural Methods to Control Foodborne Biofilms
Authors: Esposito Michelle Marie , Turku Sara
Published: December 2022

Biofilms are large aggregates of various species of bacteria or other microorganisms tightly attached to surfaces through an intricate extracellular matrix. These complex microbial communities present quite the challenge in the food processing industry, as conditions such as raw meats and diverse food product content in contact with workers, drains, machinery, and ventilation systems, make for prime circumstances for contamination. Adding to the challenge is the highly resistant nature of these biofilm growths and the need to keep in mind that any antimicrobials utilized in these situations risk health implications with human consumption of the products that are being processed in these locations. For that reason, the ideal means of sanitizing areas of foodborne biofilms would be natural means. Herein, we review a series of innovative natural methods of targeting foodborne biofilms, including bacteriocins, bacteriophages, fungi, phytochemicals, plant extracts, essential oils, gaseous and aqueous control, photocatalysis, enzymatic treatments, and ultrasound mechanisms.
Mouse Models for Mycobacterium tuberculosis Pathogenesis: Show and Do Not Tell
Authors: Soldevilla Pablo , Vilaplana Cristina , Cardona Pere-Joan
Published: December 2022

Science has been taking profit from animal models since the first translational experiments back in ancient Greece. From there, and across all history, several remarkable findings have been obtained using animal models. One of the most popular models, especially for research in infectious diseases, is the mouse. Regarding research in tuberculosis, the mouse has provided useful information about host and bacterial traits related to susceptibility to the infection. The effect of aging, sexual dimorphisms, the route of infection, genetic differences between mice lineages and unbalanced immunity scenarios upon Mycobacterium tuberculosis infection and tuberculosis development has helped, helps and will help biomedical researchers in the design of new tools for diagnosis, treatment and prevention of tuberculosis, despite various discrepancies and the lack of deep study in some areas of these traits.
Polymeric/Dextran Wafer Dressings as Promising Long-Acting Delivery Systems for Curcumin Topical Delivery and Enhancing Wound Healing in Male Wistar Albino Rats
Authors: Al Fatease Adel , Abourehab Mohammed A. S. , Alqahtani Ali M. , Chidambaram Kumarappan , Qureshi Absar Ahmed , Venkatesan Krishnaraju , Alshahrani Sultan M. , Abdelkader Hamdy
Published: December 2022

Curcumin is the main active constituent in turmeric, and it is one of the biopolyphenolic compounds. A cumulative body of research supports the use of curcumin in the treatment of wounds, yet poor water solubility and lack of therapeutic dose determination hamper its use for this therapeutic purpose. This work aimed at preparing novel curcumin wafer dressings to provide a favorable environment for wound healing. Hybrid synthetic (PVA, PVP, HPMC, and CMC) and biodegradable (sodium alginate and dextran) polymers were employed to prepare wafer dressings loaded with incremental three doses (2, 10, and 20 mg) of curcumin per a wafer dressing. The solvent casting method was used to prepare the dressings. Dimension, surface pH, mechanical properties, DSC, FTIR, XRD, erosion time, and in vitro release were studied. Skin wound healing assay was studied in Wistar albino rats. Six curcumin-loaded wafers were successfully prepared with good mechanical properties. Curcumin was dispersed in an amorphous/molecular form, as evidenced by thermal (DSC) and spectral (FTIR and XRD) analyses. Prolonged curcumin release (>24 h) was recorded for F6 (10 mg curcumin) and F7 (20 mg curcumin). Wound healing rate constants and time for 50% wound closure (T1/2) were estimated from a semi-log wound diameter versus time curve. A superior healing rate (up to 3-fold faster) was recorded for curcumin-loaded wafer dressings containing 10 mg (F6) with T1/2 of 7 days compared to 20 days for the placebo-treated group. These results warrant using the selected curcumin-loaded wafer dressing for safer and faster wound closure.
Smart Drug-Delivery System of Upconversion Nanoparticles Coated with Mesoporous Silica for Controlled Release
Authors: Huang Yanan , Du Ziqing , Bao Guochen , Fang Guocheng , Cappadona Matthew , McClements Lana , Tuch Bernard E. , Lu Hongxu , Xu Xiaoxue
Published: December 2022

Drug-delivery vehicles have garnered immense interest in recent years due to unparalleled progress made in material science and nanomedicine. However, the development of stimuli-responsive devices with controllable drug-release systems (DRSs) is still in its nascent stage. In this paper, we designed a two-way controlled drug-release system that can be promoted and prolonged, using the external stimulation of near-infrared light (NIR) and protein coating. A hierarchical nanostructure was fabricated using upconversion nanoparticles (UCNPs)—mesoporous silica as the core-shell structure with protein lysozyme coating. The mesoporous silica shell provides abundant pores for the loading of drug molecules and a specific type of photosensitive molecules. The morphology and the physical properties of the nanostructures were thoroughly characterized. The results exhibited the uniform core-shell nanostructures of ~four UCNPs encapsulated in one mesoporous silica nanoparticle. The core-shell nanoparticles were in the spherical shape with an average size of 200 nm, average surface area of 446.54 m2/g, and pore size of 4.6 nm. Using doxorubicin (DOX), a chemotherapy agent as the drug model, we demonstrated that a novel DRS with capacity of smart modulation to promote or inhibit the drug release under NIR light and protein coating, respectively. Further, we demonstrated the therapeutic effect of the designed DRSs using breast cancer cells. The reported novel controlled DRS with dual functionality could have a promising potential for chemotherapy treatment of solid cancers.
Lectin-Modified Magnetic Nano-PLGA for Photodynamic Therapy In Vivo
Authors: Kovalenko Vera L. , Komedchikova Elena N. , Sogomonyan Anna S. , Tereshina Ekaterina D. , Kolesnikova Olga A. , Mirkasymov Aziz B. , Iureva Anna M. , Zvyagin Andrei V. , Nikitin Petr I. , Shipunova Victoria O.
Published: December 2022

The extreme aggressiveness and lethality of many cancer types appeal to the problem of the development of new-generation treatment strategies based on smart materials with a mechanism of action that differs from standard treatment approaches. The targeted delivery of nanoparticles to specific cancer cell receptors is believed to be such a strategy; however, there are no targeted nano-drugs that have successfully completed clinical trials to date. To meet the challenge, we designed an alternative way to eliminate tumors in vivo. Here, we show for the first time that the targeting of lectin-equipped polymer nanoparticles to the glycosylation profile of cancer cells, followed by photodynamic therapy (PDT), is a promising strategy for the treatment of aggressive tumors. We synthesized polymer nanoparticles loaded with magnetite and a PDT agent, IR775 dye (mPLGA/IR775). The magnetite incorporation into the PLGA particle structure allows for the quantitative tracking of their accumulation in different organs and the performing of magnetic-assisted delivery, while IR775 makes fluorescent in vivo bioimaging as well as light-induced PDT possible, thus realizing the theranostics concept. To equip PLGA nanoparticles with targeting modality, the particles were conjugated with lectins of different origins, and the flow cytometry screening revealed that the most effective candidate for breast cancer cell labeling is ConA, a lectin from Canavalia ensiformis. In vivo experiments showed that after i.v. administration, mPLGA/IR775–ConA nanoparticles efficiently accumulated in the allograft tumors under the external magnetic field; produced a bright fluorescent signal for in vivo bioimaging; and led to 100% tumor growth inhibition after the single session of PDT, even for large solid tumors of more than 200 mm3 in BALB/c mice. The obtained results indicate that the mPLGA/IR775 nanostructure has great potential to become a highly effective oncotheranostic agent.
Advanced Blood–Brain Barrier Drug Delivery
Authors: Pardridge William M.
Published: December 2022

This Special Issue of Pharmaceutics, “Advanced Blood–Brain Barrier Drug Delivery,” comprises 16 articles or reviews, which cover a cross-section of brain drug delivery for either small-molecule or large-molecule therapeutics [...]
Smad7 Antisense Oligonucleotide in Crohn’s Disease: A Re-Evaluation and Explanation for the Discordant Results of Clinical Trials
Authors: Monteleone Giovanni , Stolfi Carmine
Published: December 2022

In Crohn’s disease (CD) and ulcerative colitis (UC), the major inflammatory bowel diseases (IBD) in human beings, the tissue-damaging inflammatory response is characterized by elevated levels of Suppressor of Mothers Against Decapentaplegic (Smad)7, an inhibitor of the immunosuppressive cytokine Transforming Growth Factor (TGF)-β1. Consistently, preclinical work in mouse models of IBD-like colitis showed that the knockdown of Smad7 with an antisense oligonucleotide (AS) attenuated the mucosal inflammation, thus paving the way for the development of an AS-containing pharmaceutical compound, named mongersen, for clinical use. The initial phase 1 and phase 2 studies showed that oral administration of mongersen was safe and effective in inducing clinical remission in active CD patients. However, subsequently, a large multicentered, randomized, double-blind, placebo-controlled, phase 3 trial was prematurely discontinued because of an interim analysis showing no effect of mongersen on the activity of CD. In this study we will discuss recent data showing that the majority of the batches of mongersen used in the phase 3 study were chemically different from those used in the previous clinical trials, with some of them being unable to knockdown Smad7 in cultured cells. The accumulating evidence highlights the need to maintain consistent manufacturing requirements for clinical AS, as well as the potential benefits of in vitro bioassays as a part of quality control. New clinical trials evaluating mongersen’s impact on IBD using chemically homogenous batches will be needed to ascertain the therapeutic efficacy of such a drug.
Antimetastatic Properties of Prodigiosin and the BH3-Mimetic Obatoclax (GX15-070) in Melanoma
Authors: Espona-Fiedler Margarita , Manuel-Manresa Pilar , Benítez-García Cristina , Fontova Pere , Quesada Roberto , Soto-Cerrato Vanessa , Pérez-Tomás Ricardo
Published: December 2022

Metastasis is the primary cause of death in cancer patients. Many current chemotherapeutic agents only show cytotoxic, but not antimetastatic properties. This leads to a reduction in tumor size, but allows cancer cells to disseminate, which ultimately causes patient death. Therefore, novel anticancer compounds with both effects need to be developed. In this work, we analyze the antimetastatic properties of prodigiosin and obatoclax (GX15-070), anticancer drugs of the Prodiginines (PGs) family. We studied PGs’ effects on cellular adhesion and morphology in the human primary and metastatic melanoma cell lines, SK-MEL-28 and SK-MEL-5, and in the murine melanoma cell line, B16F10A. Cell adhesion sharply decreased in the treated cells, and this was accompanied by a reduction in filopodia protrusions and a significant decrease in the number of focal-adhesion structures. Moreover, cell migration was assessed through the wound-healing assay and cell motility was severely inhibited after 24 h of treatment. To elucidate the molecular mechanisms involved, changes in metastasis-related genes were analyzed through a gene-expression array. Key genes related to cellular invasion, migration and chemoresistance were significantly down-regulated. Finally, an in vivo model of melanoma-induced lung metastasis was established and significant differences in lung tumors were observed in the obatoclax-treated mice. Altogether, these results describe, in depth, PGs’ cellular antimetastatic effects and identify in vivo antimetastatic properties of Obatoclax.
Selenium-Containing Agents Acting on Cancer—A New Hope?
Authors: Garbo Sabrina , Di Giacomo Silvia , Łażewska Dorota , Honkisz-Orzechowska Ewelina , Di Sotto Antonella , Fioravanti Rossella , Zwergel Clemens , Battistelli Cecilia
Published: December 2022

Selenium-containing agents are more and more considered as an innovative potential treatment option for cancer. Light is shed not only on the considerable advancements made in understanding the complex biology and chemistry related to selenium-containing small molecules but also on Se-nanoparticles. Numerous Se-containing agents have been widely investigated in recent years in cancer therapy in relation to tumour development and dissemination, drug delivery, multidrug resistance (MDR) and immune system-related (anti)cancer effects. Despite numerous efforts, Se-agents apart from selenocysteine and selenomethionine have not yet reached clinical trials for cancer therapy. The purpose of this review is to provide a concise critical overview of the current state of the art in the development of highly potent target-specific Se-containing agents.
Self-Reflection and Peer-Assessments Effect on Pharmacy Students’ Performance at Simulated Counselling Sessions
Authors: Bartlett Andrew , Pace Jessica , Arora Angela , Penm Jonathan
Published: December 2022

Introduction: Verbal communication is a vital skill for pharmacists and essential for improving patient care. The aim of this study was to explore students’ perception of the impact of self-reflection and self- and peer-assessment on simulated patient counselling sessions. Methods: Focus groups explored student perceptions of how this course and way of learning has impacted their performance at counselling patients. Data were analysed using iterative inductive thematic analysis procedures and mapped to the self-determination theory. Results: Nine focus groups with 47 pharmacy students. We identified three main themes and ten associated subthemes. These were learning style (sub-themes gradual introduction to assessment, learning through self-reflection videos, authentic assessment, individual learning compared to group learning, and learning through observation of best practice), feedback (sub-themes inconsistent feedback, summative feedback, perception of self and relationship with peers informing peer assessment) and benefits in real life practice. These themes mapped well to self-determination theory and highlighted that additional focus may be required for benefits in real-life practice. Conclusion: Students’ perceptions of self-reflection and self- and peer-assessment centred on learning style, feedback, and benefits in real-life practice. Additional focus on benefits of this unit of study in real-life practice and work integrated learning on placements may further strengthen the impact of these learning activities.
Achromatic Flat Metasurface Fiber Couplers within Telecom Bands
Authors: Li Jiayi , Li Rui , Xue Xiaojun , Jiang Xiao , Chen Xiaoming , Chui Hsiang-Chen
Published: December 2022

We proposed a single metalens for fiber coupling within telecom bands. This proposed fiber coupler combined a single layer metalens and a Polydimethylsiloxane (PDMS) layer. Instead of traditional fiber collimators, which are bulky and require complex calibration processes, we used a metalens for the focusing of incident and outgoing lasers and achieve achromatic aberration over a certain wavelength band. The focal length was kept as 514.9 μm with a 6.92-μm tolerance. The average coupling efficiency of an achromatic lens was calculated as 0.43. The different phases were produced with the nanopillar element structures. The aim is to provide an idea for creating a more convenient, integrated and efficient way of coupling fiber optics. This approach can also be applied to the design of achromatic lenses in other wavelength regions.
Link-Blockage Model and AP-Placement Scheme for No-Blockage Link between AGV and AP in Logistics–Warehousing VLC Network
Authors: Gong Guiyu , Gan Chaoqin , Fang Yong , Zhu Yifan , Hu Qiuyue
Published: December 2022

In this paper, the blockage problem of the optical link between AGV (autonomous ground vehicles) and AP (access point) in the logistics–warehousing VLC (visible light communication) network is analyzed. First, based on the random geometric model, a link-blockage model is proposed. Given the position of AGV, AP and obstacle, the blockage state of the VLC link between AGV and AP can be obtained through this model. Then, an AP-placement scheme based on the link-blockage model is proposed. Under this AP placement, AGVs in any position have a reliable link that is not affected by obstacles. During the movement of AGV, the VLC link of AGV will not be interrupted by a random blockage. Finally, the effectiveness of the link-blockage model is demonstrated by the shadow method. In this paper, the link outage probability and the data rate under different AP heights, AP spacings and the number of obstacles are simulated. Simulation results show that the VLC link can keep uninterrupted under the AP placement proposed in this paper.
Probability Distribution Functions of Solar and Stellar Flares
Authors: Sakurai Takashi
Published: December 2022

The paper studies the soft X-ray data of solar flares and found that the distribution functions of flare fluence are successfully modeled by tapered power law or gamma function distributions whose power exponent is slightly smaller than 2, indicating that the total energy of the flare populations is mostly due to a small number of large flares. The largest possible solar flares in 1000 years are predicted to be around X70 (a peak flux of 70 × 10−4 W m−2) in terms of the GOES (Geostationary Operational Environmental Satellites) flare class. The paper also studies superflares (more energetic than solar flares) from solar-type stars and found that their power exponent in the fitting of the gamma function distribution is around 1.05, which is much flatter than solar flares. The distribution function of stellar flare energy extrapolated downward does not connect to the distribution function of solar flare energy.
Variations in Grain Traits among Local Rice Varieties Collected More Than Half-Century Ago in Indochinese Countries
Authors: Lim Sathya , Onoda Anna , Orn Chhourn , Iwamoto Hiromu , Ishikawa Ryo , Saito Hiroki , Sato Yutaka , Ishii Takashige
Published: December 2022

More than half-century ago, local rice varieties were collected from Indochinese countries (Cambodia, Thailand, Laos, and Vietnam). Of these, 162 local varieties were examined for 7 grain-size traits: seed length/width/thickness, brown rice length/width/thickness, and 100-seed weight. Since these traits varied considerably, a survey of functional mutations was performed in the genes related to these traits. In total, 19 markers (12 InDel and 7 dCAPS markers) were used to investigate the mutations at 14 grain-size loci of GW2, GS2, qLGY3, GS3, GL3.1, TGW3, GS5, GW5, GS6, TGW6, GW6a, GLW7, GL7, and GW8. Significant allele effects were observed with six markers detecting base substitution mutations at GW2 and GS3 and insertion/deletion mutations at GS5, GW5, and GW6a, suggesting that these mutations might have affected the grain trait and caused variation among local varieties in the Indochinese countries. In addition to grain size, the hull color, grain color, and glutinosity were also examined using a survey of loss-of-function mutations at major responsible loci. Most phenotypes were reflected based on functional mutations at these loci. Since the local varieties have wide genetic variation, they are important genetic resources for future rice breeding.
Phytochemical Composition, Antioxidant and Antiproliferative Activities of Citrus hystrix, Citrus limon, Citrus pyriformis, and Citrus microcarpa Leaf Essential Oils against Human Cervical Cancer Cell Line
Authors: Othman Haneen Ibrahim Al , Alkatib Huda Hisham , Zaid Atiqah , Sasidharan Sreenivasan , Rahiman Siti Sarah Fazalul , Lee Tien Ping , Dimitrovski George , Althakafy Jalal T. , Wong Yong Foo
Published: December 2022

The essential oil derived from Citrus plants has long been used for medicinal purposes, due to its broad spectrum of therapeutic characteristics. To date, approximately 162 Citrus species have been identified, and many investigational studies have been conducted to explore the pharmacological potential of Citrus spp. oils. This study investigated the volatile constituents of essential oil distilled from the leaves of C. hystrix, C. limon, C. pyriformis, and C. microcarpa, using gas chromatography–quadrupole mass spectrometry. A total of 80 secondary compounds were tentatively identified, representing 84.88–97.99% of the total ion count and mainly comprising monoterpene (5.20–76.15%) and sesquiterpene (1.36–27.14%) hydrocarbons, oxygenated monoterpenes (3.91–89.52%) and sesquiterpenes (0.21–38.87%), and other minor chemical classes (0.10–0.52%). In particular, 27 compounds (1.19–39.06%) were detected across all Citrus species. Principal component analysis of the identified phytoconstituents and their relative quantities enabled differentiation of the Citrus leaf oils according to their species, with the loading variables contributing to these metabolic differences being identified. The Citrus leaf oils were tested for their antioxidant and antiproliferative activities using 2,2-diphenyl-1-picryl-hydrazylhydrate (DPPH) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. The results indicated that C. limon displayed the highest DPPH radical scavenging ability (IC50 value of 29.14 ± 1.97 mg/mL), while C. hystrix exhibited the lowest activity (IC50 value of 279.03 ± 10.37 mg/mL). On the other hand, all the Citrus oils exhibit potent antiproliferative activities against the HeLa cervical cancer cell line, with IC50 values of 11.66 μg/mL (C. limon), 20.41 μg/mL (C. microcarpa), 25.91 μg/mL (C. hystrix), and 87.17 μg/mL (C. pyriformis).
Large–Scale Transposon Mutagenesis Reveals Type III Secretion Effector HopR1 Is a Major Virulence Factor in Pseudomonas syringae pv. actinidiae
Authors: Ishiga Takako , Sakata Nanami , Usuki Giyu , Nguyen Viet Tru , Gomi Kenji , Ishiga Yasuhiro
Published: December 2022

Bacterial canker of kiwifruit caused by Pseudomonas syringae pv. actinidiae (Psa) is a serious threat to kiwifruit production worldwide. Four biovars (Psa biovar 1; Psa1, Psa biovar 3; Psa3, Psa biovar 5; Psa5, and Psa biovar 6; Psa6) were reported in Japan, and virulent Psa3 strains spread rapidly to kiwifruit production areas worldwide. Therefore, there is an urgent need to develop critical management strategies for bacterial canker based on dissecting the dynamic interactions between Psa and kiwifruit. To investigate the molecular mechanism of Psa3 infection, we developed a rapid and reliable high-throughput flood-inoculation method using kiwifruit seedlings. Using this inoculation method, we screened 3000 Psa3 transposon insertion mutants and identified 91 reduced virulence mutants and characterized the transposon insertion sites in these mutants. We identified seven type III secretion system mutants, and four type III secretion effectors mutants including hopR1. Mature kiwifruit leaves spray-inoculated with the hopR1 mutant showed significantly reduced virulence compared to Psa3 wild-type, indicating that HopR1 has a critical role in Psa3 virulence. Deletion mutants of hopR1 in Psa1, Psa3, Psa5, and Psa6 revealed that the type III secretion effector HopR1 is a major virulence factor in these biovars. Moreover, hopR1 mutants of Psa3 failed to reopen stomata on kiwifruit leaves, suggesting that HopR1 facilitates Psa entry through stomata into plants. Furthermore, defense related genes were highly expressed in kiwifruit plants inoculated with hopR1 mutant compared to Psa wild-type, indicating that HopR1 suppresses defense-related genes of kiwifruit. These results suggest that HopR1 universally contributes to virulence in all Psa biovars by overcoming not only stomatal-based defense, but also apoplastic defense.
Effect of Elevated CO2 and Drought on Biomass, Gas Exchange and Wood Structure of Eucalyptus grandis
Authors: Costa Layssa da Silva , Vuralhan-Eckert Jasmin , Fromm Jörg
Published: December 2022

Juvenile Eucalyptus grandis were exposed to drought and elevated CO2 to evaluate the independent and interactive effects on growth, gas exchange and wood structure. Trees were grown in a greenhouse at ambient and elevated CO2 (aCO2, 410 ppm; eCO2, 950 ppm), in combination with daily irrigation and cyclic drought during one growing season. The results demonstrated that drought stress limited intercellular CO2 concentration, photosynthesis, stomatal conductance, and transpiration, which correlated with a lower increment in height, stem diameter and biomass. Drought also induced formation of frequent and narrow vessels accompanied by a reduction in vessel lumen area. Conversely, elevated CO2 increased intercellular CO2 concentration as well as photosynthesis, and partially closed stomata, leading to a more efficient water use, especially under drought. There was a clear trend towards greater biomass accumulation at eCO2, although the results did not show statistical significance for this parameter. We observed an increase in vessel diameter and vessel lumen area at eCO2, and, contrarily, the vessel frequency decreased. Thus, we conclude that eCO2 delayed the effects of drought and potentialized growth. However, results on vessel anatomy suggest that increasing vulnerability to cavitation due to formation of larger vessels may counteract the beneficial effects of eCO2 under severe drought.
Wheat Crop under Waterlogging: Potential Soil and Plant Effects
Authors: Pais Isabel P. , Moreira Rita , Semedo José N. , Ramalho José C. , Lidon Fernando C. , Coutinho José , Maçãs Benvindo , Scotti-Campos Paula
Published: December 2022

Inundation, excessive precipitation, or inadequate field drainage can cause waterlogging of cultivated land. It is anticipated that climate change will increase the frequency, intensity, and unpredictability of flooding events. This stress affects 10–15 million hectares of wheat every year, resulting in 20–50% yield losses. Since this crop greatly sustains a population’s food demands, providing ca. 20% of the world’s energy and protein diets requirements, it is crucial to understand changes in soil and plant physiology under excess water conditions. Variations in redox potential, pH, nutrient availability, and electrical conductivity of waterlogged soil will be addressed, as well as their impacts in major plant responses, such as root system and plant development. Waterlogging effects at the leaf level will also be addressed, with a particular focus on gas exchanges, photosynthetic pigments, soluble sugars, membrane integrity, lipids, and oxidative stress.
Enhanced Organic Pollutant Removal Efficiency of Electrospun NiTiO3/TiO2-Decorated Carbon Nanofibers
Authors: Maafa Ibrahim M. , Ali Mohammad Ashraf
Published: December 2022

A nanocomposite comprised of nickel titanate/titania nanoparticles decorated with carbon nanofibers (NiTiO3/TiO2-decorated CNFs) is successfully synthesized via electrospinning and further utilized for methylene blue (MB) photodegradation. The morphology, phase, structural and chemical composition of the nanocomposite is investigated via scanning electron microscope, X-ray diffraction and transmission electron microscope equipped with energy dispersive X-ray. A mathematical model is developed to predict the photocatalytic activity of the produced nanocomposite by considering parameters such as initial dye concentration, light intensity, reaction temperature, and catalyst dosage. The reaction rate constant K1 decreased from 0.0153 to 0.0044 min−1 with an increase in the MB concentration from 5 to 15 mg L−1, while K2, K3, and K4 were found to increase with the increase in reaction temperature (0.0153 to 0.0222 min−1), light intensity (0.0153 to 0.0228 min−1) and catalyst dose concentration (0.0153 to 0.0324 min−1), respectively. The results obtained are found to be in good agreement with the modeling results and showed effective photodegradation activity. The performance of our catalyst is found to be better compared to other catalysts previously reported in the literature. The recyclability data of the synthesized NiTiO3/TiO2-decorated CNFs catalyst for four runs show that the catalyst is quite stable and recyclable. This nanocomposite photocatalyst offers a low-cost solution for wastewater pollution problems and opens new avenues to further explore the electrospinning method for the synthesis of nanocomposites.
The Dual Modification of PNIPAM and β-Cyclodextrin Grafted on Hyaluronic Acid as Self-Assembled Nanogel for Curcumin Delivery
Authors: Kaewruethai Tisana , Lin Yuan , Wang Qian , Luckanagul Jittima Amie
Published: December 2022

Curcumin is an extract of turmeric (Curcuma longa) which possesses anti-inflammatory, anti-cancer and wound-healing effects and has been used as an active compound in biomedical research for many years. However, its poor solubility presents challenges for its use in drug delivery systems. A modified nanogel delivery system, with PNIPAM and β-cyclodextrin grafted onto hyaluronic acid (PNCDHA), was utilized to enhance the solubility. The polymer was characterized by NMR, and the inclusion complex between curcumin and β-cyclodextrin was confirmed by FTIR. The potential of this PNCDHA polymer complex as a drug delivery vehicle was supported by a curcumin encapsulation efficiency of 93.14 ± 5.6% and the release of encapsulated curcumin at 37 °C. At a concentration of 0.5% w/v in water, PNCDHA nanogels were biocompatible with fibroblast cell line (L929) up to a curcumin concentration of 50 µM. There was a direct concentration between curcumin loading and cellular internalization. A more detailed study of the cellular internalization of PNCDHA nanogel should be considered in order to clarify cellular delivery mechanisms and to assess how its viability as a carrier may be optimized.
A Response Surface Methodology Approach to Improve Adhesive Bonding of Pulsed Laser Treated CFRP Composites
Authors: Mandolfino Chiara , Cassettari Lucia , Pizzorni Marco , Saccaro Stefano , Lertora Enrico
Published: December 2022

In this work, a response surface-designed experiment approach was used to determine the optimal settings of laser treatment as a method of surface preparation for CFRP prior to bonding. A nanosecond pulsed Ytterbium-doped-fiber laser source was used in combination with a scanning system. A Face-centered Central Composite Design was used to model the tensile shear strength (TSS) of adhesive bonded joints and investigate the effects of varying three parameters, namely, power, pitch, and lateral overlap. The analysis was carried out considering different focal distances. For each set of joints, shear strength values were modeled using Response Surface Methodology (RSM) to identify the set-up parameters that gave the best performance, determining any equivalent conditions from a statistical point of view. The regression models also allow the prediction of the behavior of the joints for not experimentally tested parameter settings, within the operating domain of investigation. This aspect is particularly important in consideration of the process optimization of the manufacturing cycle since it allows the maximization of joint efficiency by limiting the energy consumption for treatment.
Preparation and Characterization of Polyvinylalcohol/Polysulfone Composite Membranes for Enhanced CO2/N2 Separation
Authors: Li Ying , Chen Danlin , He Xuezhong
Published: December 2022

The unique properties of polyvinyl alcohol (PVA) and polysulfone (PSf), such as good membrane-forming ability and adjustable structure, provide a great opportunity for CO2-separation membrane development. This work focuses on the fabrication of PVA/PSf composite membranes for CO2/N2 separations. The membranes prepared by coating a 7.5 wt% PVA on top of PSf substrate showed a relatively thin selective layer of 1.7 µm with an enhanced CO2/N2 selectivity of 78, which is a ca. 200% increase compared to the pure PSf membranes. The CO2/N2 selectivity decreases at a rapid rate with the increase of feed pressure from 1.8 to 5 bar, while the CO2 permeance shows a slight reduction, which is caused by the weakening of coupling transportation between water and CO2 molecules, as well as membrane compaction at higher pressures. Increasing operating temperature from 22 °C to 50 °C leads to a slight decrease in CO2 permeance, but a significant reduction in the CO2/N2 selectivity from 78 to 27.1. Moreover, the mass transfer coefficient of gas molecules is expected to increase at a higher velocity, which leads to the increase of CO2 permeance at higher feed flow rates. It was concluded that the CO2 separation performance of the prepared membranes was significantly dependent on the membrane operating parameters, and process design and optimization are crucial to bringing CO2-separation membranes for industrial applications in post-combustion carbon capture.
A Self-Matting Waterborne Polyurethane Coating for PVC Artificial Leather
Authors: Sun Zhe , Ren Song , Wu Tong , Wen Jiating , Fang Jian , Fan Haojun
Published: December 2022

A type of polyester-based self-matting waterborne polyurethane (ESMWPU) with an excellent matte effect of 0.8° and transmittance of 78.5% for PVC artificial leather was synthesized. The influence of synthesis parameters, including R value, crosslinking and hydrophilic group content, on coating gloss and transmittance was investigated. Meanwhile, the properties necessary for applying water-based resins to plasticized PVC were detailed. The results demonstrated that R value, crosslinking degree and hydrophilic group content synergistically decided the morphological changes of latex particles during their film-forming process in three aspects: particle stiffness, three-dimensional structure and particle size, respectively. With optimized parameters of R = 1.9, TMP = 2 wt% and DMPA = 1.75 wt%, ESMWPU latex particles stabilized their spherical shapes without collapsing in the film-forming process and created a rough surface, resulting in a matte effect. In terms of application performance, good wetting and adhesion for ESMWPU to a plasticized PVC surface was achieved with a 2 wt% leveling agent load. Moreover, due to the high cohesion energy of ester bonds and intermolecular hydrogen bonds, this type of polyester-based ESMWPU also depicted admirable thermal adhesion resistance. All aforementioned results distinctly demonstrate a feasible yet promising paradigm for applying ESMWPU on PVC artificial leather.
Synthesis, Characterization, and Optimization Studies of Polycaprolactone/Polylactic Acid/Titanium Dioxide Nanoparticle/Orange Essential Oil Membranes for Biomedical Applications
Authors: Castro Jorge Ivan , Astudillo Stiven , Mina Hernandez Jose Herminsul , Saavedra Marcela , Zapata Paula A. , Valencia-Llano Carlos Humberto , Chaur Manuel N. , Grande-Tovar Carlos David
Published: December 2022

The development of scaffolds for cell regeneration has increased because they must have adequate biocompatibility and mechanical properties to be applied in tissue engineering. In this sense, incorporating nanofillers or essential oils has allowed new architectures to promote cell proliferation and regeneration of new tissue. With this goal, we prepared four membranes based on polylactic acid (PLA), polycaprolactone (PCL), titanium dioxide nanoparticles (TiO2-NPs), and orange essential oil (OEO) by the drop-casting method. The preparation of TiO2-NPs followed the sol–gel process with spherical morphology and an average size of 13.39 nm ± 2.28 nm. The results show how the TiO2-NP properties predominate over the crystallization processes, reflected in the decreasing crystallinity percentage from 5.2% to 0.6% in the membranes. On the other hand, when OEO and TiO2-NPs are introduced into a membrane, they act synergistically due to the inclusion of highly conjugated thermostable molecules and the thermal properties of TiO2-NPs. Finally, incorporating OEO and TiO2-NPs promotes tissue regeneration due to the decrease in inflammatory infiltrate and the appearance of connective tissue. These results demonstrate the great potential for biomedical applications of the membranes prepared.
Effects of Co-Solvent-Induced Self-Assembled Graphene-PVDF Composite Film on Piezoelectric Application
Authors: Widakdo Januar , Lei Wen-Ching , Anawati Anawati , Thagare Manjunatha Subrahmanya , Austria Hannah Faye M. , Setiawan Owen , Huang Tsung-Han , Chiao Yu-Hsuan , Hung Wei-Song , Ho Ming-Hua
Published: December 2022

A persistent purpose for self-powered and wearable electronic devices is the fabrication of graphene-PVDF piezoelectric nanogenerators with various co-solvents that could provide enhanced levels of durability and stability while generating a higher output. This study resulted in a piezoelectric nanogenerator based on a composite film composed of graphene, and poly (vinylidene fluoride) (PVDF) as a flexible polymer matrix that delivers high performance, flexibility, and cost-effectiveness. By adjusting the co-solvent in the solution, a graphene-PVDF piezoelectric nanogenerator can be created (acetone, THF, water, and EtOH). The solution becomes less viscous and is more diluted the more significant the concentration of co-solvents, such as acetone, THF, and EtOH. Additionally, when the density is low, the thickness will be thinner. The final film thickness for all is ~25 µm. Furthermore, the- crystal phase becomes more apparent when graphene is added and combined with the four co-solvents. Based on the XRD results, the peak changes to the right, which can be inferred to be more dominant with the β-phase. THF is the co-solvent with the highest piezoelectric output among other co-solvents. Most of the output voltages produced are 0.071 V and are more significant than the rest.
Enhance the Injection Molding Quality Prediction with Artificial Intelligence to Reach Zero-Defect Manufacturing
Authors: Silva Bruno , Marques Ruben , Faustino Dinis , Ilheu Paulo , Santos Tiago , Sousa João , Rocha André Dionisio
Published: December 2022

With the spread of the Industry 4.0 concept, implementing Artificial Intelligence approaches on the shop floor that allow companies to increase their competitiveness in the market is starting to be prioritized. Due to the complexity of the processes used in the industry, the inclusion of a real-time Quality Prediction methodology avoids a considerable number of costs to companies. This paper exposes the whole process of introducing Artificial Intelligence in plastic injection molding processes in a company in Portugal. All the implementations and methodologies used are presented, from data collection to real-time classification, such as Data Augmentation and Human-in-the-Loop labeling, among others. This approach also allows predicting and alerting with regard to process quality loss. This leads to a reduction in the production of non-compliant parts, which increases productivity and reduces costs and environmental footprint. In order to understand the applicability of this system, it was tested in different injection molding processes (traditional and stretch and blow) and with different materials and products. The results of this document show that, with the approach developed and presented, it was possible to achieve an increase in Overall Equipment Effectiveness (OEE) of up to 12%, a reduction in the process downtime of up to 9% and a significant reduction in the number of non-conforming parts produced. This improvement in key performance indicators proves the potential of this solution.
Real-World Pipe-Out Emissions from Gasoline Direct Injection Passenger Cars
Authors: Huang Junfeng , Gao Jianbing , Wang Yufeng , Yang Ce , Ma Chaochen
Published: December 2022

The analysis of real-world emissions is necessary to reduce the emissions of vehicles during on-road driving. In this paper, the matrix of gasoline direct injection passenger cars is applied to analyze the real-world emissions. The results show that high acceleration and high speed conditions are major conditions for the particulate number emissions, and the particulate number emissions are positively correlated with torque and throttle opening. The catalyst temperature and saturation are important factors that affect nitrogen oxide emission. The nitrogen oxide emissions of low speed and low torque conditions cannot be ignored in real-world driving. The carbon dioxide emissions are positively correlated with acceleration, torque and throttle opening. Once the vehicles are in the acceleration condition, the carbon dioxide emissions increase rapidly. The vehicles with higher average emission factors are more susceptible to driving behaviors, and the differences in the emission factors are more obvious, leading to an increase in the difficulty of emission control.
Parameter Optimization and Testing of a Conveying and Soil-Removing Device for Tiger Nut (Cyperus esculentus) Mechanical Harvesting
Authors: Qi Jiangtao , Pei Minghao , Kan Za , Meng Hewei
Published: December 2022

Aimed at solving the large power consumption and high operating cost problems associated with the process of harvesting tiger nuts, the use of a conveying and soil-removing device which removes sandy soil while conveying tiger nuts was proposed. The device was numerically simulated with and without vibration using EDEM software. The results showed that the vibrating force was more conducive to the complete removal of sandy soil and the effective conveyance of tiger nuts. The simulation testing was carried out using spiral speed, vibration amplitude, and vibration frequency as the independent variables and conveyance efficiency, sandy soil removal rate, etc., as the dependent variables. The test results showed that the optimal parameter combination was a spiral speed of 107 r/min, a vibration amplitude of 8.5 mm, and a vibration frequency of 10.7 Hz, under which the theoretical value of conveyance efficiency was 80.39%, the sandy soil removal rate was 84.61%, and the variation coefficient of sandy soil removal velocity was 3.6%. The field test determined that the relative errors of conveyance efficiency and sandy soil removal rate with theoretical values were 2.55% and 1.41%, respectively. The tiger nut damage rate was 1.16%, and the leakage rate was 0.52%. The results showed that the conveying and soil-removing device could meet tiger nut harvest performance requirements.
Experimental Investigation on Pyrolysis of Domestic Plastic Wastes for Fuel Grade Hydrocarbons
Authors: Ghodke Praveen Kumar , Sharma Amit Kumar , Moorthy Krishna , Chen Wei-Hsin , Patel Alok , Matsakas Leonidas
Published: December 2022

Plastics usage is rising daily because of increased population, modernization, and industrialization, which produces a lot of plastic garbage. Due to their various chemical structures, long chain polymeric compositions, and thermal/decomposition behavior, it is challenging to recycle these plastic wastes into hydrocarbon fuels. In the current work, domestic plastic waste was pyrolyzed at 473 to 973 K in a fixed bed reactor and compared with the three virgin plastics LDPE (low-density polyethylene), HDPE (high-density polyethylene), and PP (polypropylene), as well as a mixture of the three (virgin mixed plastics). The pyrolysis results showed that maximum liquid hydrocarbons obtained from HDPE, LDPE, PP, mixed plastic, and domestic waste were 64.6 wt.%, 62.2 wt.%, 63.1 wt.%, 68.6 wt.%, and 64.6 wt.% at 773 K, respectively. The composition of liquid fuels was characterized using FTIR and GC-MS, which showed a wide spectrum of hydrocarbons in the C8–C20 range. Furthermore, liquid fuel characteristics such as density, viscosity, fire and flash point, pour point, and calorific value were examined using ASTM standards, and the results were found to be satisfactory. This study provides an innovative method for recycling waste plastics into economical hydrocarbon fuel for use in transportation.
Optimization of Major Extraction Variables to Improve Recovery of Anthocyanins from Elderberry by Response Surface Methodology
Authors: Kim Seunghee , Son Hyerim , Pang So Young , Yang Jin Ju , Lee Jeongho , Lee Kang Hyun , Lee Ja Hyun , Park Chulhwan , Yoo Hah Young
Published: December 2022

Elderberry, which is well known for its richness in anthocyanin, is attracting attention in the bioindustry as a functional material with high antioxidant capacity. The aim of this study is to optimize extraction conditions to more effectively recover anthocyanins from elderberry. In a fundamental experiment to determine the suitable solvent, various GRAS reagents, such as acetone, ethanol, ethyl acetate, hexane, and isopropyl alcohol, were used, and total phenol and anthocyanin contents were detected as 9.0 mg/g-biomass and 5.1 mg/g-biomass, respectively, only in the extraction using ethanol. Therefore, ethanol was selected as the extraction solvent, and an experimental design was performed to derive a response surface model with temperature, time, and EtOH concentration as the main variables. The optimal conditions for maximal anthocyanin recovery were determined to be 20.0 °C, 15.0 min, and 40.9% ethanol, and the total anthocyanin content was 21.0 mg/g-biomass. In addition, the total phenol and flavonoid contents were detected as 67.4 mg/g-biomass and 43.8 mg/g-biomass, respectively. The very simple and economical extraction conditions suggested in this study contributed to improving the utilization potential of anthocyanin, a useful antioxidant derived from elderberry.
Feasibility Study of Applying Anaerobic Step-Feeding Mode for the Treatment of High-Strength Wastewater in Granular Sequencing Batch Reactors (GSBRs)
Authors: Mady Elsayed , Oleszkiewicz Jan , Yuan Qiuyan
Published: December 2022

This study investigated the feasibility of applying an anaerobic step-feeding strategy to enhance the performance of granular sequencing batch reactors (GSBRs) in terms of operational stability of the cultivated mature granules and nutrient removal efficiencies. Two identical 5 L reactors were operated with a total cycle time of 8 h. GSBRs were operated with high-strength synthetic wastewater (COD = 1250 ± 43, ammonium (NH4-N) = 115.2 ± 4.6, and orthophosphate (PO4-P) = 17.02 ± 0.9 mg/L) for 360 days through three stages: (1) Cultivation, 125 days (>2.1 mm); (2) Maturation, 175 days (>3 mm); (3) alternate feed loading strategy for R2 only for 60 days (anaerobic step-feeding). The granulation process, the physical properties of the granules, the nutrients, and the substrate removal performance were recorded during the entire operational period. For the cultivation and maturation stages, both reactors followed the fast single feeding mode followed by anaerobic mixing, and the results indicated a strong correlation between R1 and R2 due to the same working conditions. During the cultivation stage, adopting high organic loading rate (OLR) at the reactor start-up did not accelerate the formation of granules. Removal efficiency of PO4-P was less than 76% during the maturation period, while it exceeded 90% for COD, and was higher than 80% for NH4-N without effect of nitrite or nitrate accumulations due to simultaneous nitrification–denitrification. After changing filling mode for R2 only, there was unexpected deterioration in the performance and a rapid disintegration of the matured granules (poor settleability) accompanied by poor effluent quality due to high content of suspended solids because of applying selection pressure of short settling time. Consequently, GSBRs operation under the effect of fast single feeding mode followed by anaerobic mixing favors stable long-term granule stability.