bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Analysis of Fault-Tolerant Dual-Channel BLDC and SR Motors
Authors: Korkosz M. , Bogusz P. , Prokop J. , Pakla B. , Podskarbi G.
Published: June 2019

This article presents the results of a comparative analysis of two electronically commutated brushless direct current machines intended for fault-tolerant drives. Two machines designed by the authors were compared: a 12/14 dual-channel brushless direct current motor (DCBLDCM) with permanent magnets and a 12/8 dual-channel switched reluctance motor (DCSRM). Information is provided here on the winding configuration, the parameters, and the power converters of both machines. We developed mathematical models of the DCBLDCM and DCSRM which accounted for the nonlinearity of their magnetization characteristics in dual-channel operation (DCO) and single-channel operation (SCO) modes. The static torque characteristics and flux characteristics of both machines were compared for operation in DCO and SCO modes. The waveforms of the current and the electromagnetic torque are presented for DCO and SCO operating conditions. For DCO mode, an analysis of the behavior of both machines under fault conditions (i.e., asymmetrical control, shorted coil, and open phase) was performed. The two designs were compared, and their strengths and weaknesses were indicated.
Providing Ancillary Services with Wind Turbine Generators Based on DFIG with a Two-Branch Static Converter
Authors: Morais Ernande Eugenio C. , Lima Francisco Kleber de A. , Fonseca Jean M. L. , Branco Carlos G. C. , Machado Lívia de A.
Published: June 2019

This work aims to analyze and validate through mathematical modeling and experimental results, in a three-phase three-wire electrical system, the technical viability of a static power converter with a two-level topology with only two controlled branches (2L2B), operating as a grid-side converter (GSC) in a wind turbine generator based on a doubly fed induction generator (DFIG). With this reduced-switches topology, the GSC is able to regulate the DC-link voltage level from the generator back-to-back converter and provide ancillary services of harmonic filtering and reactive power compensation from linear/nonlinear loads connected to the point of common coupling. An 8-kVA experimental prototype was implemented in the laboratory to validate the proposal. The prototype control system was realized using the dSPACE DS1103 PPC Controller Board platform programmed via MATLAB/Simulink. The effectiveness of the proposed system is verified by comparing the results obtained with the 2L2B topology to the ones with the usual two-level three-branch topology.
State of Charge Estimation for Power Lithium-Ion Battery Using a Fuzzy Logic Sliding Mode Observer
Authors: Zheng Wenhui , Xia Bizhong , Wang Wei , Lai Yongzhi , Wang Mingwang , Wang Huawen
Published: June 2019

State of charge (SOC) estimation is of vital importance for the battery management system in electric vehicles. This paper proposes a new fuzzy logic sliding mode observer for SOC estimation. The second-order resistor-capacitor equivalent circuit model is used to describe the discharging/charging behavior of the battery. The exponential fitting method is applied to determine the parameters of the model. The fuzzy logic controller is introduced to improve the performance of sliding mode observer forming the fuzzy logic sliding mode observer (FLSMO). The Federal Urban Driving Schedule (FUDS), the West Virginia Suburban Driving Schedule (WUBSUB), and the New European Driving Cycle (NEDC) schedule test results show that the average SOC estimation error of FLSMO algorithm is less than 1%. When the initial SOC estimation error is 20%, the FLSMO algorithm can converge to 3% error boundary within 2400 s. Comparison test results show that the FLSMO algorithm has better performance than the sliding mode observer and the extended Kalman filter in terms of robustness against measurement noise and parameter disturbances.
Open-Switch Fault Diagnosis Algorithm and Tolerant Control Method of the Three-Phase Three-Level NPC Active Rectifier
Authors: Jung Jun-Hyung , Ku Hyun-Keun , Son Yung-Deug , Kim Jang-Mok
Published: June 2019

This paper proposes a fault diagnosis and tolerant control methods for an open-switch fault caused in a three-phase three-level neutral-point-clamped (NPC) pulse-width modulation (PWM) active rectifier. The open-switch fault in the three-level NPC active rectifier causes a distortion in the input phase current and a ripple in the DC-link capacitor voltage. Therefore, proper fault diagnosis and tolerant control methods are required to prevent additional failures and performance degradation in the rectifier system. This paper conducted a detailed analysis of the effect of the single open-switch fault on the NPC PWM active rectifier and proposed a fault diagnosis method utilizing the DC link voltage and the phase angle of the input grid voltage. Furthermore, this paper proposes a fault-tolerant control method to reduce the effect of the open-switch fault by compensating a distorted reference voltage. The effectiveness of the proposed fault diagnosis and tolerant control methods are verified through experimental results.
Determination of the Lignocellulosic Components of Olive Tree Pruning Biomass by Near Infrared Spectroscopy
Authors: Fernández José Luis , Sáez Felicia , Castro Eulogio , Manzanares Paloma , Ballesteros Mercedes , Negro María José
Published: June 2019

The determination of chemical composition of lignocellulose biomass by wet chemistry analysis is labor-intensive, expensive, and time consuming. Near infrared (NIR) spectroscopy coupled with multivariate calibration offers a rapid and no-destructive alternative method. The objective of this work is to develop a NIR calibration model for olive tree lignocellulosic biomass as a rapid tool and alternative method for chemical characterization of olive tree pruning over current wet methods. In this study, 79 milled olive tree pruning samples were analyzed for extractives, lignin, cellulose, hemicellulose, and ash content. These samples were scanned by reflectance diffuse near infrared techniques and a predictive model based on partial least squares (PLS) multivariate calibration method was developed. Five parameters were calibrated: Lignin, cellulose, hemicellulose, ash, and extractives. NIR models obtained were able to predict main components composition with R2cv values over 0.5, except for lignin which showed lowest prediction accuracy.
Measuring the Labile and Recalcitrant Pools of Carbon and Nitrogen in Forested and Agricultural Soils: A Study under Tropical Conditions
Authors: Ferraz de Almeida Risely , Rodrigues Mikhael Joseph Elias , Oliveira Franco Fernando , Fonseca Santana Luna Monique , Wendling Beno
Published: June 2019

Soil organic carbon and nitrogen can be divided into labile and recalcitrant pools according to the time it takes to be cycled. The way in which carbon and nitrogen pools are cycled and distributed between labile and recalcitrant pools can directly relate to soil quality. This paper tested the hypothesis that labile and recalcitrant pools of carbon and nitrogen vary between agricultural soils with different species and fertilization management systems (nitrogen, phosphorus, and potassium need) under tropical conditions. This study aimed to examine the impact of land-uses on stocks and losses of carbon and nitrogen under tropical conditions. We explored labile (soil microbial biomass and labile carbon) and recalcitrant carbon pools (humin, humic acid, and fulvic acid) in forested and agricultural soils, defined as latosol (forest, fertilized pasture, and unfertilized pasture) and cambisol (forest, coast pasture, sugarcane, and silage corn). Forested soil was used as an appropriate use to soil conservation in tropical that presents levels adequate of carbon and nitrogen stocks and biological condition in soil. Results showed that pools of labile and recalcitrant carbon are different on soil layers and the use of soil. Forest use in cambisol and latosol promoted higher labile and recalcitrant pools of carbon and nitrogen due to the greater environmental stability without human intervention. On the other hand, human intervention occurred in fertilized pasture and coast pasture; however, both uses presented similar recalcitrant carbon and nitrogen pools when compared to forested soil on the soil surface due to fertilizer uses and the high volume of the grass root system. Overall, our findings reveal that under tropical conditions, agriculture and forested soil can present similar recalcitrant pools of carbon and nitrogen if agricultural soils are associated with the appropriate fertilizer management. Pasture with adequate fertilization management systems can be used as an alternative to recover degraded areas with low levels of recalcitrant carbon and nitrogen pools.
Effect of Different Processing Methods on the Accumulation of the Phenolic Compounds and Antioxidant Profile of Broomcorn Millet (Panicum miliaceum L.) Flour
Authors: Kalam Azad Md Obyedul , Jeong Da In , Adnan Md , Salitxay Timnoy , Heo Jeong Won , Naznin Most Tahera , Lim Jung Dae , Cho Dong Ha , Park Byoung Jae , Park Cheol Ho
Published: June 2019

Broomcorn millet (Panicum miliaceum L.) is an important nutritious ancient minor-cereal food crop. However, this crop is little explored in the food processing arena to improve its functionality. In this context, different processing methods were applied to enhance the secondary compounds of broomcorn millet. Four different individual methods such as roasting, steaming, puffing, and extrusion were applied at 110 °C to enhance the functional attributes of millet flour. It was observed that the significantly highest content of total phenolic (TP) (670 mg/100 g of ferulic acid equivalent) and total flavonoid (TF) (391 mg/100 g of rutin equivalent ) was attained in the roasted whole millet followed by steaming (315 mg/100 g, 282 mg/100 g), puffing (645 mg/100 g, 304 mg/100 g), extrusion (455 mg/100 g, 219 mg/100 g), and control (295 mg/100 g, 183 mg/100 g). The chromatographic analysis showed a greater content of single phenolic acids such as syringic acid, gallic acid, 4-hydroxy benzoic acid, ferulic acid, sinapic acid, and catechin in roasted millet compared to control, and the content of each acid was higher in whole millet than dehulled. Results also indicated that the content of ferulic acid was relatively higher among the quantified single phenolic acid from broomcorn millet. Likewise, in comparison with dehulled millet, the roasted whole millet showed higher total antioxidant capacity, measured by the 2,2-diphenyl-1 picryl hydrazyl (DPPH), the ferric reducing antioxidant power assay (FRAP), the phosphomolybdenum method (PPMD), and the hydroxyl radical scavenging capacity (HRSC) method. Lastly, it is concluded that the roasting method should be taken into consideration in the processing of broomcorn millet to enhance the content of nutraceutical compounds and improve its functionality.
Screening the Reference Genes for Quantitative Gene Expression by RT-qPCR During SE Initial Dedifferentiation in Four Gossypium hirsutum Cultivars that Have Different SE Capability
Authors: Cao Aiping , Shao Dongnan , Cui Baiming , Tong Xuecheng , Zheng Yinying , Sun Jie , Li Hongbin
Published: June 2019

RNA sequencing (RNA-Seq)-based gene expression analysis is applicable to a wide range of biological purposes in various species. Reverse transcription quantitative PCR (RT-qPCR) is also used to assess target gene expression utilizing stably expressed reference genes as internal control under a given set of conditions. However, investigations of the reference genes for RT-qPCR normalization in the process of somatic embryogenesis (SE) initial dedifferentiation in Gossypium hirsutum are rarely reported. In this study, on the basis of our previous transcriptome data of three different induction stages during SE initial dedifferentiation process in four G. hirsutum cultivars that have different SE capability, 15 candidate genes were selected during SE initial dedifferentiation process, and their expression stability was evaluated by geNorm, NormFinder, and BestKeeper. The results indicated that the two genes of endonuclease 4 (ENDO4) and 18S ribosomal RNA (18S rRNA) showed stable expression in the four different G. hirsutum cultivars, endowing them to be appropriate reference genes during three induction stages in the four cotton cultivars. In addition, the stability and reliability of the two reference genes of ENDO4 and 18S rRNA were further verified by comparing the expressions of auxin-responsive protein 22 (AUX22) and ethylene-responsive transcription factor 17 (ERF17) between RT-qPCR results and the RNA-seq data, which showed strong positive correlation coefficient (R2 = 0.8396–0.9984), validating again the steady expression of ENDO4 and 18S rRNA as the reliable reference genes. Our results provide effective reference genes for RT-qPCR normalization during SE process in different G. hirsutum cultivars.
Decreased Temperature Sensitivity of Vestigial Gene Expression in Temperate Populations of Drosophila melanogaster
Authors: Voigt Susanne , Erpf Anna Christina , Stephan Wolfgang
Published: June 2019

Drosophila melanogaster recently spread from its tropical origin in Africa and became a cosmopolitan species that has adapted to a wide range of different thermal environments, including temperate climates. An important limiting factor of temperate climates has probably been their low and varying temperatures. The transcriptional output of genes can vary across temperatures, which might have been detrimental while settling in temperate environments. The reduction of temperature-sensitive expression of functionally important genes to ensure consistent levels of gene expression might have been relevant while adapting to such environments. In this study, we focus on the gene vestigial (vg) whose product is a key factor in wing development. We provide evidence that temperature-sensitivity of vg has been buffered in populations from temperate climates. We investigated temperature-sensitivity of vg gene expression in six natural populations, including four temperate populations (three from Europe and one from high-altitude Africa), and two tropical populations from the ancestral species range. All temperate populations exhibited a lower degree of temperature-induced expression plasticity than the tropical populations.
The Initial Questionnaire Development in Measuring of Coach-Athlete–Parent Interpersonal Relationships: Results of Two Qualitative Investigations
Authors: Lisinskiene Ausra , May Emily , Lochbaum Marc
Published: June 2019

The interpersonal relationship among the coach, athlete, and parent (C-A-P) in youth sports is a complex and dynamic phenomenon. The evaluation of such interpersonal relationship becomes an important factor in trying to understand the overall youth sports environment. The purpose of this study was to begin the development of a questionnaire to assess the coach-athlete–parent interpersonal relationships in youth sports. To achieve our purpose, two qualitative studies were conducted. In the first qualitative study, 136 research participants completed an online questionnaire asking for statements concerning the C-A-P based on an extensive literature review. In the second phase, a follow up phenomenological study design was conducted. For the phenomenological study, 30 participants (10 coaches, 10 athletes, and 10 youth sports parents) completed in-depth interviews. Based on both qualitative study results, the following three themes emerged: group processes, motivation, and over-involvement. The two qualitative investigations revealed themes and 48 potential questions to be used in developing a C-A-P questionnaire in quantifying the C-A-P results.
Personal NO2 and Volatile Organic Compounds Exposure Levels are Associated with Markers of Cardiovascular Risk in Women in the Cape Town Region of South Africa
Authors: Everson Frans , De Boever Patrick , Nawrot Tim S. , Goswami Nandu , Mthethwa Mashudu , Webster Ingrid , Martens Dries S. , Mashele Nyiko , Charania Sana , Kamau Festus , Strijdom Hans
Published: June 2019

Exposure to ambient NO2 and benzene, toluene ethyl-benzene and m+p- and o-xylenes (BTEX) is associated with adverse cardiovascular effects, but limited information is available on the effects of personal exposure to these compounds in South African populations. This 6-month follow-up study aims to determine 7-day personal ambient NO2 and BTEX exposure levels via compact passive diffusion samplers in female participants from Cape Town, and investigate whether exposure levels are associated with cardiovascular risk markers. Overall, the measured air pollutant exposure levels were lower compared to international standards. NO2 was positively associated with systolic and diastolic blood pressure (SBP and DBP), and inversely associated with the central retinal venular equivalent (CRVE) and mean baseline brachial artery diameter. o-xylene was associated with DBP and benzene was strongly associated with carotid intima media thickness (cIMT). Our findings showed that personal air pollution exposure, even at relatively low levels, was associated with several markers of cardiovascular risk in women residing in the Cape Town region.
Groundwater Chemistry and Blood Pressure: A Cross-Sectional Study in Bangladesh
Authors: Naser Abu Mohd , Clasen Thomas F. , Luby Stephen P. , Rahman Mahbubur , Unicomb Leanne , Ahmed Kazi M. , Doza Solaiman , Ourshalimian Shadassa , Chang Howard H. , Stowell Jennifer D. , Narayan K. M. Venkat , Shamsudduha Mohammad , Patel Shivani A. , O’Shea Bethany , Gribble Matthew O.
Published: June 2019

Background: We assessed the association of groundwater chemicals with systolic blood pressure (SBP) and diastolic blood pressure (DBP). Methods: Blood pressure data for ≥35-year-olds were from the Bangladesh Demographic and Health Survey in 2011. Groundwater chemicals in 3534 well water samples from Bangladesh were measured by the British Geological Survey (BGS) in 1998–1999. Participants who reported groundwater as their primary source of drinking water were assigned chemical measures from the nearest BGS well. Survey-adjusted linear regression methods were used to assess the association of each groundwater chemical with the log-transformed blood pressure of the participants. Models were adjusted for age, sex, body mass index, smoking status, geographical region, household wealth, rural or urban residence, and educational attainment, and further adjusted for all other groundwater chemicals. Results: One standard deviation (SD) increase in groundwater magnesium was associated with a 0.992 (95% confidence interval (CI): 0.986, 0.998) geometric mean ratio (GMR) of SBP and a 0.991 (95% CI: 0.985, 0.996) GMR of DBP when adjusted for covariates except groundwater chemicals. When additionally adjusted for groundwater chemicals, one SD increase in groundwater magnesium was associated with a 0.984 (95% CI: 0.972, 0.997) GMR of SBP and a 0.990 (95% CI: 0.979, 1.000) GMR of DBP. However, associations were attenuated following Bonferroni-correction for multiple chemical comparisons in the full-adjusted model. Groundwater concentrations of calcium, potassium, silicon, sulfate, barium, zinc, manganese, and iron were not associated with SBP or DBP in the full-adjusted models. Conclusions: Groundwater magnesium had a weak association with lower SBP and DBP of the participants.
The Korea Cancer Big Data Platform (K-CBP) for Cancer Research
Authors: Cha Hyo Soung , Jung Jip Min , Shin Seob Yoon , Jang Young Mi , Park Phillip , Lee Jae Wook , Chung Seung Hyun , Choi Kui Son
Published: June 2019

Data warehousing is the most important technology to address recent advances in precision medicine. However, a generic clinical data warehouse does not address unstructured and insufficient data. In precision medicine, it is essential to develop a platform that can collect and utilize data. Data were collected from electronic medical records, genomic sequences, tumor biopsy specimens, and national cancer control initiative databases in the National Cancer Center (NCC), Korea. Data were de-identified and stored in a safe and independent space. Unstructured clinical data were standardized and incorporated into cancer registries and linked to cancer genome sequences and tumor biopsy specimens. Finally, national cancer control initiative data from the public domain were independently organized and linked to cancer registries. We constructed a system for integrating and providing various cancer data called the Korea Cancer Big Data Platform (K-CBP). Although the K-CBP could be used for cancer research, the legal and regulatory aspects of data distribution and usage need to be addressed first. Nonetheless, the system will continue collecting data from cancer-related resources that will hopefully facilitate precision-based research.
Well-Being: Its Relationship with Work-to-Family Conflict and Burnout among Males and Females
Authors: Huang Shu-Ling , Li Ren-Hau , Fang Shu-Yi , Tang Feng-Cheng
Published: June 2019

The present study aims to apply gender-specific analyses to examine how work-to-family conflict (WFC) and burnout are related to well-being among the workers in Taiwan. A cross-sectional research design was adopted. A questionnaire was distributed to obtain information pertaining to demographic characteristics, WFC, burnout, and well-being. In total, 4259 full-time workers in the manufacturing industry were recruited. Gender-specific statistical analyses were used. The results showed that no significant gender difference occurred on WFC; however, females had higher scores on burnout compared to males. In the correlation analyses, WFC as well as burnout were negatively associated with well-being in both genders. In the regression analyses when demographic factors were controlled, burnout explained larger variances of well-being in both genders compared with WFC. WFC made a smaller contribution to the models predicting well-being in males in contrast to females. Moreover, the significant association between WFC and well-being for males disappeared when burnout was taken into account. The conclusion reached was that to improve workers’ well-being, organizations should develop relevant policies to decrease the extent of burnout for different genders. The policies that the organization adopted should consider females’ needs beyond work-related burden. Moreover, merely decreasing the extent of WFC is insufficient to enhance males’ well-being.
Night Shift Work, DNA Methylation and Telomere Length: An Investigation on Hospital Female Nurses
Authors: Carugno Michele , Maggioni Cristina , Crespi Eleonora , Bonzini Matteo , Cuocina Simone , Dioni Laura , Tarantini Letizia , Consonni Dario , Ferrari Luca , Pesatori Angela Cecilia
Published: June 2019

Increased breast cancer risk has been reported in some night shift (NS) workers but underlying biological mechanisms are still unclear. We assessed the association between NS work and DNA methylation of tumor suppressor (TP53, CDKN2A, BRCA1, BRCA2) and estrogen receptor (ESR1, ESR2) genes, methylation of repetitive elements (LINE-1, Alu), and telomere length (TL). Forty six female nurses employed in NS for at least two years were matched by age (30–45 years) and length of service (≥1 year) with 51 female colleagues not working in NS. Each subject underwent a semi-structured interview and gave a blood sample. We applied linear regression and spline models adjusted for age, BMI, smoking habit, oral contraceptive use, parity and marital status/age at marriage. Currently working in NS was associated with ESR1 hypomethylation (β: −1.85 (95%CI: −3.03; −0.67), p = 0.003). In current and former NS workers we observed TP53 (−0.93 (−1.73; −0.12), p = 0.03) and BRCA1 (−1.14 (−1.71; −0.58), p <0.001) hypomethylation. We found an increase between TL and number of years in NS in subjects employed in NS <12 years (0.06 (0.03; 0.09), p <0.001), while a decrease if employed in NS ≥12 years (−0.07 −0.10; −0.04), p <0.001). Our findings show NS-associated markers potentially involved in cellular aging, genomic instability, and cancer development.
Food Insecurity and Geriatric Hospitalization
Authors: Bergmans Rachel S. , Mezuk Briana , Zivin Kara
Published: June 2019

Food insecurity (FI) has been associated with hospitalization, although the pathways underlying this relationship are poorly understood, in part due to the potential for a bidirectional relationship. This study aimed to determine associations of FI with concurrent and future hospitalization among older adults; mediation by depression and; whether hospitalization increased risk of FI. Participants came from the 2012 and 2014 waves of the Health and Retirement Study (HRS; n = 13,664). HRS is a prospective cohort representative of U.S. adults over the age of 50. Primary analyses included those who were not hospitalized in 2012 (n = 11,776). Not having enough money to buy necessary food or eating less than desired defined food insecurity. The Composite International Diagnostic Interview Short Form provided depression symptomology. Logistic and linear regression examined concurrent and longitudinal associations of FI in 2012 and 2014 with hospitalization in 2014. Path analysis tested mediation of FI with hospitalization frequency by depression symptomology. Finally, logistic regression examined whether hospitalization in 2012 was longitudinally associated with FI in 2014. FI was not associated with future hospitalization (odds ratio (OR) = 1.1; 95% confidence interval (CI) = 0.9–1.4), however; FI was associated with concurrent hospitalization status (OR = 1.4; 95% CI = 1.1–1.8). Depression symptomology explained 17.4% (95% CI = 2.8–32.0%) the association of FI with concurrent hospitalization frequency. Additionally, hospitalization was associated with becoming food insecure (OR = 1.5; 95% CI = 1.2–2.0). Findings may inform best practices for hospital discharge among older adults.
Influence of Physical Education Teachers on Motivation, Embarrassment and the Intention of Being Physically Active During Adolescence
Authors: Trigueros Rubén , Aguilar-Parra José M. , Cangas Adolfo J. , López-Liria Remedios , Álvarez Joaquín F.
Published: June 2019

According to a WHO report (2018), more than 80% of adolescents do not do enough physical activity. Physical Education (PE) classes should be aimed at solving this problem. The present study aims to analyze the influence teachers have on motivation, embarrassment and intention to be physically active among their students. A total of 604 secondary school students participated in the study. Various statistical analyses were carried out to explain the causal relationships between the variables. The results revealed a positive relation between the autonomy support and the satisfaction of basic psychological needs (BPN), and a negative relation with the frustration of BPN. In contrast, perceived control revealed a positive relation with frustration of BPN, and a negative relation with the satisfaction of BPN. Satisfaction of BPN was negatively related to embarrassment and positively related to self-determined motivation. On the other hand, frustration of BPN was positively related to embarrassment and negatively with self-determined motivation. Embarrassment was negatively related to self-determined motivation, and the latter was positively related to intention to be physically active. Indeed, the study demonstrates the influence and the importance of PE teachers and of the motivational and emotional processes of adolescents during PE classes and the role they play in acquiring the habits of an active lifestyle.
Modeling and Predicting Dengue Incidence in Highly Vulnerable Countries using Panel Data Approach
Authors: Anwar Asim , Khan Noman , Ayub Muhammad , Nawaz Faisal , Shah Asim , Flahault Antoine
Published: June 2019

The spread of dengue has become a major public health concern in recent times due to alarming climate change. Using country level panel data over the 2000–2017 period, this paper examines the effects of climate change and socio-economic variables on the incidence of dengue-borne diseases in some of the most highly vulnerable countries. Empirical analysis shows a positive association between climate change and socio-economic conditions in the advent of dengue-borne diseases. We find that climate change, as measured by temperature, is proactively contributing to the spread of dengue-borne diseases. However, redressing the contributive factor behind climate change, via better awareness through education and improved public health facilitation, can assist in managing the occurrences and spread of dengue-borne diseases.
Characteristics of Authigenic Minerals around the Sulfate-Methane Transition Zone in the Methane-Rich Sediments of the Northern South China Sea: Inorganic Geochemical Evidence
Authors: Wu Daidai , Sun Tiantian , Xie Rui , Pan Mengdi , Chen Xuegang , Ye Ying , Liu Lihua , Wu Nengyou
Published: June 2019

Sediments at marine cold seep areas provide potential archives of past fluid flow, which allow insights into the evolution of past methane seepage activities. However, signals for anaerobic oxidation of methane (AOM) might be obscured in bulk sediments in cold-seep settings due to several factors, especially flood and turbidite deposition. Comprehensive inorganic data were gathered in this study to explore the availability of related records at cold seeps and to provide insights into the evolution of past methane seepage activities. Sediments collected from the site 973-4 in the Taixinan Basin on the northern slope of the South China Sea were characterized in terms of total carbon and sulfur, δ13C values of total organic carbon (δ13CTIC), δ34S values of chromium reducible sulfur (δ34SCRS), and foraminiferal oxygen and carbon isotopes. The results confirmed a strong correlation between formation of authigenic minerals and AOM. Moreover, the 34S enrichments and abundant chromium reducible sulfur (CRS) contents in the authigenic sulfides in the sulfate–methane transition zone (SMTZ) within 619–900 cm below seafloor (cmbsf) reflected past high methane fluxes supported by constant methane seepages. Lithological distribution and AMS (Accelerator Mass Spectra) 14C dating of planktonic foraminifera show that the turbidite (~35.14 ka) was related to a foraminifera-rich interval (Unit II: 440-619 cmbsf) and increased carbonate productivity during the last glacial maximum (LGM). Enrichment of Mo and U was observed accompanied by low contents of nutrient metals (Al, Ti, V, Ni, Fe, Mn, and Cu) in Unit II. The foraminifera-rich interval (Unit II) of cold seep sediments was probably linked to the phenomenon of inconsecutive sedimentary sequence due to the turbidites, which resulted in the lack of Fe, Mn, and Ba enrichment. There is no U enrichment but only Mo enrichment within Unit III, which might be related to H2S produced by AOM during the methane seepages. Based on the above results, it can be speculated that this area has experienced multiple-episodes of methane seep events. Further exploration of AOM should focus on the risks of rapid deposition, especially the impact of turbidity current on sediments.
Multi-Indicator Approach for Characterising Urban Green Space Provision at City and City-District Level in Germany
Authors: Grunewald Karsten , Richter Benjamin , Behnisch Martin
Published: June 2019

This paper addresses the question of how a sustainable urban development can be supported through simple measured quantities in the context of the specific provision of green space and open space. The specific provision of green space is analysed based on a combination of six indicators that describe, on the one hand, the access of inhabitants to green spaces and on the other hand, the settlement character as well as the strong anthropogenic imprint on the urban landscape. The indicators were calculated and combined in a 9-cell matrix for classifying the areas studied. The implementation was carried out at two scales for all German cities with at least 50,000 inhabitants as well as exemplarily for city districts of eight big cities. The calculated indicator values for representing green characteristics decrease with increasing number of inhabitants, whereas the opposite relationship was obtained for the indicators of the grey characteristics. We show how the approach provides an in-depth morphological assessment of German cities ranking their scores from low to the high presence of grey/green characteristics.
Evaluation of Disease Severity and Global Transcriptome Response Induced by Citrus bark cracking viroid, Hop latent viroid, and Their Co-Infection in Hop (Humulus lupulus L.)
Authors: Štajner Nataša , Radišek Sebastjan , Mishra Ajay Kumar , Nath Vishnu Sukumari , Matoušek Jaroslav , Jakše Jernej
Published: June 2019

Viroids are small non-capsidated, single-stranded, covalently-closed circular noncoding RNA replicons of 239–401 nucleotides that exploit host factors for their replication, and some cause disease in several economically important crop plants, while others appear to be benign. The proposed mechanisms of viroid pathogenesis include direct interaction of the genomic viroid RNA with host factors and post-transcriptional or transcriptional gene silencing via viroid-derived small RNAs (vd-sRNAs) generated by the host defensive machinery. Humulus lupulus (hop) plants are hosts to several viroids among which Hop latent viroid (HLVd) and Citrus bark cracking viroid (CBCVd) are attractive model systems for the study of viroid-host interactions due to the symptomless infection of the former and severe symptoms induced by the latter in this indicator host. To better understand their interactions with hop plant, a comparative transcriptomic analysis based on RNA sequencing (RNA-seq) was performed to reveal the transcriptional alterations induced as a result of single HLVd and CBCVd infection in hop. Additionally, the effect of HLVd on the aggressiveness of CBCVd that underlies severe stunting in hop in a mixed infection was studied by transcriptomic analysis. Our analysis revealed that CBCVd infection resulted in dynamic changes in the activity of genes as compared to single HLVd infection and their mixed infection. The differentially expressed genes that are involved in defense, phytohormone signaling, photosynthesis and chloroplasts, RNA regulation, processing and binding; protein metabolism and modification; and other mechanisms were more modulated in the CBCVd infection of hop. Nevertheless, Gene Ontology (GO) classification and pathway enrichment analysis showed that the expression of genes involved in the proteolysis mechanism is more active in a mixed infection as compared to a single one, suggesting co-infecting viroids may result in interference with host factors more prominently. Collectively, our results provide a deep transcriptome of hop and insight into complex single HLVd, CBCVd, and their coinfection in hop-plant interactions
Real Microgravity Influences the Cytoskeleton and Focal Adhesions in Human Breast Cancer Cells
Authors: Nassef Mohamed Zakaria , Kopp Sascha , Wehland Markus , Melnik Daniela , Sahana Jayashree , Krüger Marcus , Corydon Thomas J. , Oltmann Hergen , Schmitz Burkhard , Schütte Andreas , Bauer Thomas J. , Infanger Manfred , Grimm Daniela
Published: June 2019

With the increasing number of spaceflights, it is crucial to understand the changes occurring in human cells exposed to real microgravity (r-µg) conditions. We tested the effect of r-µg on MCF-7 breast cancer cells with the objective to investigate cytoskeletal alterations and early changes in the gene expression of factors belonging to the cytoskeleton, extracellular matrix, focal adhesion, and cytokines. In the Technische Experimente unter Schwerelosigkeit (TEXUS) 54 rocket mission, we had the opportunity to conduct our experiment during 6 min of r-µg and focused on cytoskeletal alterations of MCF-7 breast cancer cells expressing the Lifeact-GFP marker protein for the visualization of F-actin as well as the mCherry-tubulin fusion protein using the Fluorescence Microscopy Analysis System (FLUMIAS) for fast live-cell imaging under r-µg. Moreover, in a second mission we investigated changes in RNA transcription and morphology in breast cancer cells exposed to parabolic flight (PF) maneuvers (31st Deutsches Zentrum für Luft- und Raumfahrt (DLR) PF campaign). The MCF-7 cells showed a rearrangement of the F-actin and tubulin with holes, accumulations in the tubulin network, and the appearance of filopodia- and lamellipodia-like structures in the F-actin cytoskeleton shortly after the beginning of the r-µg period. PF maneuvers induced an early up-regulation of KRT8, RDX, TIMP1, CXCL8 mRNAs, and a down-regulation of VCL after the first parabola. E-cadherin protein was significantly reduced and is involved in cell adhesion processes, and plays a significant role in tumorigenesis. Changes in the E-cadherin protein synthesis can lead to tumor progression. Pathway analyses indicate that VCL protein has an activating effect on CDH1. In conclusion, live-cell imaging visualized similar changes as those occurring in thyroid cancer cells in r-µg. This result indicates the presence of a common mechanism of gravity perception and sensation.
Chlorogenic Acid Alleviates Thiram-Induced Tibial Dyschondroplasia by Modulating Caspases, BECN1 Expression and ECM Degradation
Authors: Zhang Jialu , Huang Shucheng , Tong Xiaole , Zhang Lihong , Jiang Xiong , Zhang Hui , Mehmood Khalid , Li Jiakui
Published: June 2019

Chlorogenic acid (CGA) is a widely applied traditional Chinese medicine ingredient which can be used for the treatment of osteoporosis. In this experiment, we investigated the potential therapeutic effect of chlorogenic acid on thiram-induced tibial dyschondroplasia (TD) and explored the underlying mechanisms that have been rarely mentioned by others yet. Performance indicator analysis and tibial parameter analysis showed that CGA exhibited a definite positive effect on thiram-induced TD chickens. In order to further explore the mechanisms underlying the positive actions of CGA, apoptotic, autophagic genes and MMPs involved in matrix mineralization of growth plate were evaluated in this study. The results showed that CGA decreased the expression of pro-apoptotic genes caspases-3 and caspases-9, leading to the reduction of apoptotic cells accumulated in growth plate. In addition, CGA also increased the level of BECN1, an important gene involved in autophagy, which benefits the survival of abnormal cells. Furthermore, CGA also increased the expression of MMP-9, MMP-10, and MMP-13, which can directly affect the ossification of bones. Altogether, these results demonstrate that CGA possesses a positive therapeutic effect on thiram-induced TD via modulating the expression of caspases and BECN1 and regulating the degradation of ECM (extracellular matrix).
The Role of Soil Fungi in K+ Plant Nutrition
Authors: Haro Rosario , Benito Begoña
Published: June 2019

K+ is an essential cation and the most abundant in plant cells. After N, its corresponding element, K, is the nutrient required in the largest amounts by plants. Despite the numerous roles of K in crop production, improvements in the uptake and efficiency of use of K have not been major focuses in conventional or transgenic breeding studies in the past. In research on the mineral nutrition of plants in general, and K in particular, this nutrient has been shown to be essential to soil-dwelling-microorganisms (fungi, bacteria, protozoa, nematodes, etc.) that form mutualistic associations and that can influence the availability of mineral nutrients for plants. Therefore, this article aims to provide an overview of the role of soil microorganisms in supplying K+ to plants, considering both the potassium-solubilizing microorganisms and the potassium-facilitating microorganisms that are in close contact with the roots of plants. These microorganisms can influence the active transporter-mediated transfer of K+. Regarding the latter group of microorganisms, special focus is placed on the role of endophytic fungus. This review also includes a discussion on productivity through sustainable agriculture.
Meta-Analysis of Microarray Expression Studies on Metformin in Cancer Cell Lines
Authors: Schulten Hans-Juergen , Bakhashab Sherin
Published: June 2019

Several studies have demonstrated that metformin (MTF) acts with variable efficiency as an anticancer agent. The pleiotropic anticancer effects of MTF on cancer cells have not been fully explored yet. By interrogating the Gene Expression Omnibus (GEO) for microarray expression data, we identified eight eligible submissions, representing five different studies, that employed various conditions including different cell lines, MTF concentrations, treatment durations, and cellular components. A compilation of the data sets of 13 different conditions contained 443 repeatedly up- and 387 repeatedly down-regulated genes; the majority of these 830 differentially expressed genes (DEGs) were associated with higher MTF concentrations and longer MTF treatment. The most frequently upregulated genes include DNA damage inducible transcript 4 (DDIT4), chromodomain helicase DNA binding protein 2 (CHD2), endoplasmic reticulum to nucleus signaling 1 (ERN1), and growth differentiation factor 15 (GDF15). The most commonly downregulated genes include arrestin domain containing 4 (ARRDC4), and thioredoxin interacting protein (TXNIP). The most significantly (p-value < 0.05, Fisher’s exact test) overrepresented protein class was entitled, nucleic acid binding. Cholesterol biosynthesis and other metabolic pathways were specifically affected by downregulated pathway molecules. In addition, cell cycle pathways were significantly related to the data set. Generated networks were significantly related to, e.g., carbohydrate and lipid metabolism, cancer, cell cycle, and DNA replication, recombination, and repair. A second compilation comprised genes that were at least under one condition up- and in at least another condition down-regulated. Herein, the most frequently deregulated genes include nuclear paraspeckle assembly transcript 1 (NEAT1) and insulin induced gene 1 (INSIG1). The most significantly overrepresented protein classes in this compilation were entitled, nucleic acid binding, ubiquitin-protein ligase, and mRNA processing factor. In conclusion, this study provides a comprehensive list of deregulated genes and biofunctions related to in vitro MTF application and individual responses to different conditions. Biofunctions affected by MTF include, e.g., cholesterol synthesis and other metabolic pathways, cell cycle, and DNA replication, recombination, and repair. These findings can assist in defining the conditions in which MTF exerts additive or synergistic effects in cancer treatment.
Renin Activity in Heart Failure with Reduced Systolic Function—New Insights
Authors: Sullivan Ryan D. , Mehta Radhika M. , Tripathi Ranjana , Reed Guy L. , Gladysheva Inna P.
Published: June 2019

Regardless of the cause, symptomatic heart failure (HF) with reduced ejection fraction (rEF) is characterized by pathological activation of the renin–angiotensin–aldosterone system (RAAS) with sodium retention and extracellular fluid expansion (edema). Here, we review the role of active renin, a crucial, upstream enzymatic regulator of the RAAS, as a prognostic and diagnostic plasma biomarker of heart failure with reduced ejection fraction (HFrEF) progression; we also discuss its potential as a pharmacological bio-target in HF therapy. Clinical and experimental studies indicate that plasma renin activity is elevated with symptomatic HFrEF with edema in patients, as well as in companion animals and experimental models of HF. Plasma renin activity levels are also reported to be elevated in patients and animals with rEF before the development of symptomatic HF. Modulation of renin activity in experimental HF significantly reduces edema formation and the progression of systolic dysfunction and improves survival. Thus, specific assessment and targeting of elevated renin activity may enhance diagnostic and therapeutic precision to improve outcomes in appropriate patients with HFrEF.
Morphological, Transcriptomic and Hormonal Characterization of Trimonoecious and Subandroecious Pumpkin (Cucurbita maxima) Suggests Important Roles of Ethylene in Sex Expression
Authors: Wang Yunli , Yan Chundong , Zou Bingxue , Wang Chaojie , Xu Wenlong , Cui Chongshi , Qu Shuping
Published: June 2019

Sex expression is a complex process, and in-depth knowledge of its mechanism in pumpkin is important. In this study, young shoot apices at the one-true-leaf stage and 10-leaf stage in Cucurbita maxima trimonoecious line ‘2013–12’ and subandroecious line ‘9–6’ were collected as materials, and transcriptome sequencing was performed using an Illumina HiSeqTM 2000 System. 496 up-regulated genes and 375 down-regulated genes were identified between shoot apices containing mostly male flower buds and only female flower buds. Based on gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, the differentially expressed genes were mainly enriched in the ethylene and auxin synthesis and signal transduction pathways. In addition, shoot apices at the 4-leaf stage were treated with the ethylene-releasing agent 2-chloroethylphosphonic acid (Ethrel), aminoethoxyvinyl glycine (AVG), AgNO3 and indoleacetic acid (IAA). The number of female flowers up to node 20 on the main stem of ‘2013–12’ increased significantly after Ethrel and IAA treatment and decreased significantly after AVG and AgNO3 treatment. The female flowers in ‘9–6’ showed slight changes after treatment with the exogenous chemicals. The expression of key genes in ethylene synthesis and signal transduction (CmaACS7, CmaACO1, CmaETR1 and CmaEIN3) was determined using quantitative RT-PCR, and the expression of these four genes was positively correlated with the number of female flowers in ‘2013–12’. The variations in gene expression, especially that of CmaACS7, after chemical treatment were small in ‘9–6’. From stage 1 (S1) to stage 7 (S7) of flower development, the expression of CmaACS7 in the stamen was much lower than that in the ovary, stigma and style. These transcriptome data and chemical treatment results indicated that IAA might affect pumpkin sex expression by inducing CmaACS7 expression and indirectly affecting ethylene production, and the ethylene synthesis and signal transduction pathways play crucial roles in pumpkin flower sex expression. A possible reason for the differences in sex expression between pumpkin lines ‘2013–12’ and ‘9–6’ was proposed based on the key gene expression. Overall, these transcriptome data and chemical treatment results suggest important roles for ethylene in pumpkin sex expression.