bluuebunny/crossref_metadata_2025 huggingface dataset:
Low-Sloped Rooftop Storm-Water Detention Assembly to Mitigate Urban Flooding
Authors: Jandaghian Zahra , Zhu Yingxin , Saragosa James , Doshi Hitesh , Baskaran Bas
Published: December 2022

Low-sloped roofs such as commercial and high-rise buildings in a dense urban area provide vast “unused” surfaces that can be used to manage storm-water and mitigate urban flooding. Storm-water Detention Assembly (SDA) known as “blue roof” exerts advanced drainage technologies and operational strategies to store-and-release storm-water during and after heavy rainfall events. SDA can reduce peak flow and decrease storm-water run-off volume. However, the SDA application is limited due to the lack of science-based regulatory requirements. This paper introduces SDA, summarizes the benefits and challenges of this system, reviews the existing code specifications on roofing drain systems, and documents the criteria to design and construct SDA. With this systematic approach, the missing links are identified that shall form code change request for possible inclusion in the National Building Code of Canada (NBCC) and National Plumping Code of Canada (NPC). In addition, a road map is stated to calculate and benchmark the drain requirements on low-sloped roofs for a given design climatic load.
Green Home Buying Intention of Malaysian Millennials: An Extension of Theory of Planned Behaviour
Authors: Masukujjaman Mohammad , Wang Cheng-Kun , Alam Syed Shah , Lin Chieh-Yu , Ho Yi-Hui , Siddik Abu Bakkar
Published: December 2022

The empirical study aimed to examine the factors affecting green home buying intention in Malaysia. The study adopted the popular theory of individual decision-making, the theory of planned behaviour, but extended it with a few new variables to get better predictability. Based on the cross-sectional survey method and quota sampling technique, the instrument-like questionnaire was distributed to the potential buyers who had the intention to buy a home. Data were collected from 741 potential buyers who are young Malaysian students. Collected data were analysed using partial least squares (PLS) modelling (SmartPLS 3.0) in order to measure the strength of the independent variable effects on the dependent variable. The results indicate that attitude, perceived behavioural control subjective norms, perceived value, reasonable pricing and green building knowledge had a positive and significant effect on green home buying intention, with attitude showing the highest effect; the only exception is an environmental concern. This study also validated the theory of planned behaviour (TPB), which provides important insight for the firms involved with the housing development industry for their future business strategy.
Squeeze-MNet: Precise Skin Cancer Detection Model for Low Computing IoT Devices Using Transfer Learning
Authors: Shinde Rupali Kiran , Alam Md. Shahinur , Hossain Md. Biddut , Md Imtiaz Shariar , Kim JoonHyun , Padwal Anuja Anil , Kim Nam
Published: December 2022

Cancer remains a deadly disease. We developed a lightweight, accurate, general-purpose deep learning algorithm for skin cancer classification. Squeeze-MNet combines a Squeeze algorithm for digital hair removal during preprocessing and a MobileNet deep learning model with predefined weights. The Squeeze algorithm extracts important image features from the image, and the black-hat filter operation removes noise. The MobileNet model (with a dense neural network) was developed using the International Skin Imaging Collaboration (ISIC) dataset to fine-tune the model. The proposed model is lightweight; the prototype was tested on a Raspberry Pi 4 Internet of Things device with a Neo pixel 8-bit LED ring; a medical doctor validated the device. The average precision (AP) for benign and malignant diagnoses was 99.76% and 98.02%, respectively. Using our approach, the required dataset size decreased by 66%. The hair removal algorithm increased the accuracy of skin cancer detection to 99.36% with the ISIC dataset. The area under the receiver operating curve was 98.9%.
The Lymphatic Endothelium in the Context of Radioimmuno-Oncology
Authors: Suárez Lucía , Rodríguez-Ruiz María E. , Rouzaut Ana
Published: December 2022

The study of lymphatic tumor vasculature has been gaining interest in the context of cancer immunotherapy. These vessels constitute conduits for immune cells’ transit toward the lymph nodes, and they endow tumors with routes to metastasize to the lymph nodes and, from them, toward distant sites. In addition, this vasculature participates in the modulation of the immune response directly through the interaction with tumor-infiltrating leukocytes and indirectly through the secretion of cytokines and chemokines that attract leukocytes and tumor cells. Radiotherapy constitutes the therapeutic option for more than 50% of solid tumors. Besides impacting transformed cells, RT affects stromal cells such as endothelial and immune cells. Mature lymphatic endothelial cells are resistant to RT, but we do not know to what extent RT may affect tumor-aberrant lymphatics. RT compromises lymphatic integrity and functionality, and it is a risk factor to the onset of lymphedema, a condition characterized by deficient lymphatic drainage and compromised tissue homeostasis. This review aims to provide evidence of RT’s effects on tumor vessels, particularly on lymphatic endothelial cell physiology and immune properties. We will also explore the therapeutic options available so far to modulate signaling through lymphatic endothelial cell receptors and their repercussions on tumor immune cells in the context of cancer. There is a need for careful consideration of the RT dosage to come to terms with the participation of the lymphatic vasculature in anti-tumor response. Here, we provide new approaches to enhance the contribution of the lymphatic endothelium to radioimmuno-oncology.
TP-0903 Is Active in Preclinical Models of Acute Myeloid Leukemia with TP53 Mutation/Deletion
Authors: Eisenmann Eric D. , Stromatt Jack C. , Fobare Sydney , Huang Kevin M. , Buelow Daelynn R. , Orwick Shelley , Jeon Jae Yoon , Weber Robert H. , Larsen Bill , Mims Alice S. , Hertlein Erin , Byrd John C. , Baker Sharyn D.
Published: December 2022

Acute myeloid leukemia (AML) with mutations in the tumor suppressor gene TP53 confers a dismal prognosis with 3-year overall survival of <5%. While inhibition of kinases involved in cell cycle regulation induces synthetic lethality in a variety of TP53 mutant cancers, this strategy has not been evaluated in mutant TP53 AML. Previously, we demonstrated that TP-0903 is a novel multikinase inhibitor with low nM activity against AURKA/B, Chk1/2, and other cell cycle regulators. Here, we evaluated the preclinical activity of TP-0903 in TP53 mutant AML cell lines, including a single-cell clone of MV4-11 containing a TP53 mutation (R248W), Kasumi-1 (R248Q), and HL-60 (TP 53 null). TP-0903 inhibited cell viability (IC50, 12–32 nM) and induced apoptosis at 50 nM. By immunoblot, 50 nM TP-0903 upregulated pChk1/2 and pH2AX, suggesting induction of DNA damage. The combination of TP-0903 and decitabine was additive in vitro, and in vivo significantly prolonged median survival compared to single-agent treatments in mice xenografted with HL-60 (vehicle, 46 days; decitabine, 55 days; TP-0903, 63 days; combination, 75 days) or MV4-11 (R248W) (51 days; 62 days; 81 days; 89 days) (p < 0.001). Together, these results provide scientific premise for the clinical evaluation of TP-0903 in combination with decitabine in TP53 mutant AML.
Apoptosis as a Barrier against CIN and Aneuploidy
Authors: Weiss Johannes , Gallob Filip , Rieder Patricia , Villunger Andreas
Published: December 2022

Aneuploidy is the gain or loss of entire chromosomes, chromosome arms or fragments. Over 100 years ago, aneuploidy was described to be a feature of cancer and is now known to be present in 68–90% of malignancies. Aneuploidy promotes cancer growth, reduces therapy response and frequently worsens prognosis. Chromosomal instability (CIN) is recognized as the main cause of aneuploidy. CIN itself is a dynamic but stochastic process consisting of different DNA content-altering events. These can include impaired replication fidelity and insufficient clearance of DNA damage as well as chromosomal mis-segregation, micronuclei formation, chromothripsis or cytokinesis failure. All these events can disembogue in segmental, structural and numerical chromosome alterations. While low levels of CIN can foster malignant disease, high levels frequently trigger cell death, which supports the “aneuploidy paradox” that refers to the intrinsically negative impact of a highly aberrant karyotype on cellular fitness. Here, we review how the cellular response to CIN and aneuploidy can drive the clearance of karyotypically unstable cells through the induction of apoptosis. Furthermore, we discuss the different modes of p53 activation triggered in response to mitotic perturbations that can potentially trigger CIN and/or aneuploidy.
“OPERATION PHALCO”—Adapted Physical Activity for Breast Cancer Survivors: Is It Time for a Multidisciplinary Approach?
Authors: Murri Arianna , Vitucci Daniela , Tranchita Eliana , Grazioli Elisa , Gori Stefania , Modena Alessandra , Turazza Monica , Filippini Roberto , Galeazzi Silvia , Verzè Matteo , Frittelli Patrizia , Corsi Domenico Cristiano , Nicolis Fabrizio , Parisi Attilio , Cerulli Claudia
Published: December 2022

The international literature emphasizes the importance of physical activity (PA) in the first steps after cancer surgery. The regular practice of physical exercise causes positive adaptations on several functional capacities, with positive consequences on patients’ quality of life. This project aims to evaluate the effect of a post-operative training protocol, structured by taking into account both cancer-related issues and the presence of comorbidities, on functional capacities and quality of life of breast cancer survivors. Therefore, it was necessary to create a synergy between oncologists (referring physicians), sport medicine physicians (risk stratification and exercise prescription) and kinesiologists (trainers). Thirty-five post-surgery BC patients decided on a voluntary basis to attend an online Adapted PA (APA) protocol for 4 months, twice a week (APA Group) or Usual Care Group (UC Group). Functional capacity of the APA Group significantly increased, by 13.1% (p = 0.000), whereas perceived exertion decreased by 19.7% (p = 0.020). In the same group, the general health evaluated through the questionnaire EORTC-QLQ-C30 increased (p = 0.050). No differences were found in the UC Group. Operation Phalco, creating a network between oncologists, sports medicine physicians and kinesiologists, confirms the importance of structuring a post-operative path where APA should be included as early as possible in the cancer patient care.
Solvent-Free Oxidation of Benzyl Alcohol Derivatives by In Situ Generated Redox Couple Pd(0)/PdOx Supported on Ceria Nanorods
Authors: Moeini Seyed Sepehr , Tuti Simonetta , Battocchio Chiara , Luisetto Igor , Tofani Daniela
Published: December 2022

Benzyl alcohol (BnOH) oxidation to benzaldehyde (PhCHO) is a pivotal industrial reaction. The aerobic oxidation of BnOH in solvent-free conditions is highly compatible with the necessity of low environmental impact. In this research work, palladium oxide (PdOx) supported on ceria nanorods (CeO2-NR), was synthesized, and utilized for aerobic solvent-free oxidation of BnOH derivatives to the corresponding aldehydes. The catalyst, PdOx/CeO2-NR, was characterized by X-ray diffraction (XRD), field-emission scanning electron microscopy/energy-dispersive spectroscopy (FE-SEM/EDS), N2 adsorption-desorption analysis, temperature-programmed reduction with hydrogen (H2-TPR), and X-ray Photoelectron Spectroscopy (XPS), proving that the PdOx (x > 1) particles were highly dispersed on CeO2-NR and have a strong interaction with the support. The PdOx/CeO2-NR catalyst permitted the aerobic oxidation of various benzyl alcohol derivatives with good conversion, and high selectivity towards the corresponding aldehydes. The presence of electron donating groups (EDG) on the benzylic ring enhanced the reactivity as opposed to the electron withdrawing groups (EWG) which were detrimental for the catalytic activity. During the reaction a partial reduction of the metal produced a Pd(0)/PdOx/CeO2-NR redox couple stable in the reaction condition, more reactive and recyclable. Some mechanistic hypotheses are presented.
Genome-Wide Analysis on Transcriptome and Methylome in Prevention of Mammary Tumor Induced by Early Life Combined Botanicals
Authors: Arora Itika , Li Shizhao , Crowley Michael R. , Li Yuanyuan , Tollefsbol Trygve O.
Published: December 2022

Breast cancer (BC) is the most common malignancy and the second leading cause of cancer death among women in the United States. The consumption of natural dietary components such as broccoli sprouts (BSp) and green tea polyphenols (GTPs) has demonstrated exciting potential in reducing the risk of BC through the regulation of epigenetic mechanisms. However, little is known about their impacts on reversing epigenomic aberrations that are centrally involved in the initiation and progression of BC. Previously, we have determined the efficacy of combined BSp and GTPs treatment on the inhibition of the growth of a mammary tumor in a transgenic Her2/neu mouse model. We sought to extend our previous study to identify universal biomarkers that represent common mechanistic changes among different mouse models in response to this dietary regime by including a new transgenic mouse model, C3(1)-SV40 TAg (SV40). As a result, we identified novel target genes that were differentially expressed and methylated in response to dietary botanicals when administered singly (BSp and GTPs) and in combination (BSp + GTPs) in both mouse models. We discovered more differentially expressed and methylated genes in the combination treatment group compared to the singly administered groups. Subsequently, several biological pathways related to epigenetic regulations were identified in response to the combination treatment. Furthermore, when compared to the BSp and GTPs treatment alone, the combinatorial treatment showed a more significant impact on the regulation of the epigenetic modifier activities involved in DNA methylation and histone modifications. Our study provides key insights about the impact of the combined administration of BSp and GTPs on BC using a multi-omics analysis, suggesting a combinatorial approach is more efficacious in preventing and inhibiting BC by impacting key tumor-related genes at transcriptomic and methylomic levels. Our findings could be further extrapolated as a comprehensive source for understanding the epigenetic modifications that are associated with the effects of these dietary botanicals on BC prevention.
Mitochondrial Unfolded Protein Response and Integrated Stress Response as Promising Therapeutic Targets for Mitochondrial Diseases
Authors: Lu Hedong , Wang Xiaolei , Li Min , Ji Dongmei , Liang Dan , Liang Chunmei , Liu Yajing , Zhang Zhiguo , Cao Yunxia , Zou Weiwei
Published: December 2022

The development and application of high-throughput omics technologies have enabled a more in-depth understanding of mitochondrial biosynthesis metabolism and the pathogenesis of mitochondrial diseases. In accordance with this, a host of new treatments for mitochondrial disease are emerging. As an essential pathway in maintaining mitochondrial proteostasis, the mitochondrial unfolded protein response (UPRmt) is not only of considerable significance for mitochondrial substance metabolism but also plays a fundamental role in the development of mitochondrial diseases. Furthermore, in mammals, the integrated stress response (ISR) and UPRmt are strongly coupled, functioning together to maintain mitochondrial function. Therefore, ISR and UPRmt show great application prospects in the treatment of mitochondrial diseases. In this review, we provide an overview of the molecular mechanisms of ISR and UPRmt and focus on them as potential targets for mitochondrial disease therapy.
NAD+ Metabolism and Interventions in Premature Renal Aging and Chronic Kidney Disease
Authors: Chanvillard Lucie , Tammaro Alessandra , Sorrentino Vincenzo
Published: December 2022

Premature aging causes morphological and functional changes in the kidney, leading to chronic kidney disease (CKD). CKD is a global public health issue with far-reaching consequences, including cardio-vascular complications, increased frailty, shortened lifespan and a heightened risk of kidney failure. Dialysis or transplantation are lifesaving therapies, but they can also be debilitating. Currently, no cure is available for CKD, despite ongoing efforts to identify clinical biomarkers of premature renal aging and molecular pathways of disease progression. Kidney proximal tubular epithelial cells (PTECs) have high energy demand, and disruption of their energy homeostasis has been linked to the progression of kidney disease. Consequently, metabolic reprogramming of PTECs is gaining interest as a therapeutic tool. Preclinical and clinical evidence is emerging that NAD+ homeostasis, crucial for PTECs’ oxidative metabolism, is impaired in CKD, and administration of dietary NAD+ precursors could have a prophylactic role against age-related kidney disease. This review describes the biology of NAD+ in the kidney, including its precursors and cellular roles, and discusses the importance of NAD+ homeostasis for renal health. Furthermore, we provide a comprehensive summary of preclinical and clinical studies aimed at increasing NAD+ levels in premature renal aging and CKD.
Identification of Differentially Expressed Genes Related to the Lipid Metabolism of Esophageal Squamous Cell Carcinoma by Integrated Bioinformatics Analysis
Authors: Cui Meng-Ying , Yi Xing , Zhu Dan-Xia , Wu Jun
Published: December 2022

Purpose: In recent years, lipid metabolism has been reprogrammed to meet the energy and substrate needs of tumorigenesis and development and is a potential new target for cancer treatment. However, the regulatory mechanism of lipid metabolism in esophageal squamous cell carcinoma is not well understood. Methods: We first downloaded the esophageal squamous cell carcinoma (ESCC) gene dataset in the GEO and TCGA databases and analyzed the central differentially expressed genes (DEGs) of ESCC through bioinformatics. Afterwards, the GSEA method was used to analyze the lipid metabolism-related pathway of the central gene in the pathological process of ESCC, and it was determined that the central gene OIP5 was significantly related to the fatty acid metabolism pathway. Our heatmap also revealed that the enrichment of the ACSL family in ESCC tissues was more pronounced than in normal tissues. We hypothesized that OIP5 can regulate the fatty acid metabolism process in ESCC cells and affect the tumorigenic ability of ESCC. Further statistical analysis and experiment were conducted to determine the lipid metabolism-related gene, OIP5′s, expression pattern and clinical significance in ESCC, analyze the effect of OIP5 expression on fatty acid metabolism-related enzymes in ESCC, revealing the specific mechanism of OIP5 that promotes ESCC development. Conclusions: Our study established a correlation between OIP5 expression and clinicopathological factors (tumor size, T stage, N stage, and clinical grade) in esophageal squamous cell carcinoma (p < 0.05). We have also experimentally demonstrated that OIP5 regulates ESCC fatty acid metabolism by influencing the expression of the key enzyme ACSL1 in lipid metabolism.
Cancer Care during the COVID-19 Pandemic: Challenges and Adaptations
Authors: Ahmed Shahid
Published: December 2022

The COVID-19 pandemic is an unprecedented event that has had both acute and long-lasting effects on cancer care [...]
The Evidence Surrounding Non-Alcoholic Fatty Liver Disease in Individuals with Cancer: A Systematic Literature Review
Authors: George Elena S. , Sood Surbhi , Kiss Nicole , Daly Robin M. , Nicoll Amanda J. , Roberts Stuart K. , Baguley Brenton J.
Published: December 2022

Emerging evidence indicates an association between non-alcoholic fatty liver disease (NAFLD), cancer development and mortality. Cancer treatment-induced metabolic and hepatic dysfunction may be associated with increased rates of NAFLD. The review aims to investigate current evidence surrounding NAFLD in adults (≥18 years) with cancer including prevalence, effect of cancer treatments, metabolic co-morbidities, and mortality. Embase, Scopus, PubMed, and CINAHL were searched from inception to December 2021 including randomized controlled trials and observational studies. Twenty-three articles were included, comprising 142,218 participants. The overall risk of bias for observational studies was determined as low for 10 studies and neutral for 12 studies, and the RCT was determined as some concerns. The prevalence of NAFLD, based on imaging or histology, in adults with cancer ranged from 0.5 to 81.3%, with higher prevalence in breast, colorectal and gynecological cancers. Higher rates of NAFLD were also seen in patients who (i) underwent treatments—including chemotherapy and hormone therapy and/or who (ii) had higher BMI or other metabolic co-morbidities. NAFLD was associated with an increase in all-cause and cancer-related mortality. Based on review results, it is recommended that further assessment is carried out to determine whether liver screening in high-risk patients is cost effective and if interventions can be implemented to improve hepatic and health outcomes in adults with cancer.
Overexpression of AKR1B10 Predicts Poor Prognosis in Gastric Cancer Patients Undergoing Surgical Resection
Authors: Liu Yu-Yin , Liu Yueh-Wei , Huang Gong-Kai , Hung Kuo-Chen , Lin Yu-Hung , Yeh Cheng-Hsi , Yin Shih-Min , Tsai Ching-Hua , Chen Yen-Hao
Published: December 2022

Aldo–keto reductase family 1 member B10 (AKR1B10) is associated with several cancers, but the prognostic role in gastric cancer (GC) remains unclear. We enrolled 359 GC patients who underwent a gastrectomy with D2 lymph node dissection. AKR1B10 expression was scored using an immunoreactive scoring system based on immunohistochemistry. Adjuvant chemotherapy with S-1 or oxaliplatin plus capecitabine was administered to pathological stage II or III disease patients. There were 117 (32.6%) and 242 (67.4%) patients with AKR1B10 overexpression and low expression, respectively. Patients overexpressing AKR1B10 had worse 5-year disease-free survival (DFS) and overall survival (OS) rates than those with low expression of AKR1B10. Pathological T3–T4 stage, pathological stage III, lymph node ratio ≥25%, and AKR1B10 overexpression were independent prognostic factors for worse DFS and OS in univariate and multivariate analyses. For 162 stage II or III patients who received adjuvant chemotherapy after surgical resection and 59 patients with signet ring cell carcinoma histology, AKR1B10 overexpression was also associated with inferior DFS and OS. AKR1B10 was not associated with clinical survival in stage I GC patients. In conclusion, AKR1B10 overexpression may be an independent prognostic factor for worse survival in GC patients who underwent gastrectomy with D2 lymph node dissection.
Coral Oasis on Con Dao Islands: A Potential Refuge of Healthy Corals in the Offshore Waters of Vietnam?
Authors: Tkachenko K. S. , Dung V. V. , Ha V. T.
Published: December 2022

Coral reefs of Vietnam are highly threatened by a combination of anthropogenic impacts and natural disturbances. As a result, preservation of the remaining reefs is a major governmental concern. Con Dao Islands, located in the coastal area of southern Vietnam in the South China Sea, still possess diverse and healthy coral communities. Coral surveys conducted in 2017–2020 on six sites within the marine protected area of Con Dao National Park revealed extensive coral cover (62.8–95.5%) and diversity (168 stony coral species). Coral communities were mostly dominated by Acroporidae followed by Poritidae and Fungiidae. Temporal dynamics over a 3-year period exhibited no significant decrease in the cover of dominant coral taxa, despite the severe thermal anomaly in 2019 and subsequent moderate coral bleaching, suggesting that the local corals may be successfully acclimating to the current level of thermal stress, although further study of coral adaptation in this region is warranted. High diversity and coral cover, together with the potential of resistance and resilience to repeated thermal stress in coral communities of the Con Dao Archipelago, highlights the need for authorities to pay special attention to this area and to expand conservational efforts to preserve this unique natural site.
Challenges for Artificial Intelligence in Recognizing Mental Disorders
Authors: Yan Wen-Jing , Ruan Qian-Nan , Jiang Ke
Published: December 2022

Artificial Intelligence (AI) appears to be making important advances in the prediction and diagnosis of mental disorders. Researchers have used visual, acoustic, verbal, and physiological features to train models to predict or aid in the diagnosis, with some success. However, such systems are rarely applied in clinical practice, mainly because of the many challenges that currently exist. First, mental disorders such as depression are highly subjective, with complex symptoms, individual differences, and strong socio-cultural ties, meaning that their diagnosis requires comprehensive consideration. Second, there are many problems with the current samples, such as artificiality, poor ecological validity, small sample size, and mandatory category simplification. In addition, annotations may be too subjective to meet the requirements of professional clinicians. Moreover, multimodal information does not solve the current challenges, and within-group variations are greater than between-group characteristics, also posing significant challenges for recognition. In conclusion, current AI is still far from effectively recognizing mental disorders and cannot replace clinicians’ diagnoses in the near future. The real challenge for AI-based mental disorder diagnosis is not a technical one, nor is it wholly about data, but rather our overall understanding of mental disorders in general.
SARS-CoV-2 Infection: What Is Currently Known about Homocysteine Involvement?
Authors: Filip Nina , Cojocaru Elena , Badulescu Oana Viola , Clim Andreea , Pinzariu Alin Constantin , Bordeianu Gabriela , Jehac Alina Elena , Iancu Cristina Elena , Filip Cristiana , Maranduca Minela Aida , Sova Ivona Andreea , Serban Ionela Lacramioara
Published: December 2022

Since December 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread rapidly throughout the world causing health, social and economic instability. The severity and prognosis of patients with SARS-CoV-2 infection are associated with the presence of comorbidities such as cardiovascular disease, hypertension, chronic lung disease, cerebrovascular disease, diabetes, chronic kidney disease, and malignancy. Thrombosis is one of the most serious complications that can occur in patients with COVID-19. Homocysteine is a non-proteinogenic α-amino acid considered a potential marker of thrombotic diseases. Our review aims to provide an updated analysis of the data on the involvement of homocysteine in COVID-19 to highlight the correlation of this amino acid with disease severity and the possible mechanisms by which it intervenes.
Numerical Simulation of Multiarea Seepage in Deep Condensate Gas Reservoirs with Natural Fractures
Authors: Zhang Lijun , Bu Wengang , Li Nan , Tan Xianhong , Liu Yuwei
Published: December 2022

Research into condensate gas reservoirs in the oil and gas industry has been paid much attention and has great research value. There are also many deep condensate gas reservoirs, which is of great significance for exploitation. In this paper, the seepage performance of deep condensate gas reservoirs with natural fractures was studied. Considering that the composition of condensate gas changes during the production process, the component model was used to describe the condensate gas seepage in the fractured reservoir, modeled using the discrete fracture method, and the finite element method was used to conduct numerical simulation to analyze the seepage dynamic. The results show that the advancing speed of the moving pressure boundary can be reduced by 55% due to the existence of threshold pressure gradient. Due to the high-speed flow effect in the near wellbore area, as well as the high mobility of oil, the condensate oil saturation near the wellbore can be reduced by 42.8%. The existence of discrete natural fractures is conducive to improving the degree of formation utilization and producing condensate oil.
Nucleation Process of the 2017 Nuugaatsiaq, Greenland Landslide
Authors: Guo Zhenwei , Hou Xinrong , Gao Dawei , Liu Jianxin
Published: December 2022

Seismic precursors prior to the failure of rocks are essential for probing the nucleation process and mitigating hazards. However, such precursory events before large landslides are rarely reported possibly due to the lack of near-source observations. The 2017 Nuugaatsiaq, Greenland landslide that was preceded by an abundance of small earthquakes and captured by a local seismic station is a notable exception and offers us a valuable opportunity to investigate how a large landslide initiated. Prior work suggests that accelerated creeping plays an important role during the landslide nucleation process. However, by analyzing the temporal evolution of the waveform similarities, waveform amplitudes, and inter-event times of the seismic precursors, we find that the Nuugaatsiaq landslide was very likely triggered by a series of accelerated and migratory small earthquakes approaching the nucleation area of the upcoming landslide, thus providing important insights into the failure initiation of massive landslides.
Soil Seed Banks of Dry Tropical Forests under Different Land Management
Authors: Bravo Sandra Josefina del Valle , Abdala Nelly Roxana , Ibáñez-Moro Amalia Valeria
Published: December 2022

The objectives of this study were to evaluate woody-species composition and seed density in the soil seed bank (SSB) in a dry subtropical forest with different disturbance regimes and assess the role of leaf litter as a seed reservoir in disturbed forests. Study area: the western Argentine Chaco region. Climate is seasonal and semiarid, and the fire season coincides with the dry and cool periods. In the first step, we evaluated the composition of species and seed density in the soils of forests with four different combinations of disturbances (wildfires, livestock, roller-chopping, and logging) using a systematic sampling design. In the second step, we assessed the seed density in the soil and litter fractions under focal individuals of six native woody species in two forest types (undisturbed/disturbed by roller-chopper and wildfires). Soil samples were extracted by core following standard methods for SSB studies. Eleven woody species were found in the SSB. The seed density varied between 17.78 seeds/samples in the reference condition forest and 5.46 seeds/samples in the more intensively disturbed forest (wildfires and livestock). The tree seed abundance was reduced in the disturbed forest SSB and the shrubby species increased. There were no significant differences in the seed density among the soil and litter fractions of each type of forest, but disturbances reduced the seed density in the litter fraction. The leaf litter is a seed reservoir in the soils of the Chaco forests, but this localization could promote loss by fires and desiccation. Our results could improve the forestry management plans in areas exposed to climate and land-use change.
Different Environmental and Phylogenetic Controls over the Altitudinal Variation in Leaf N and P Resorption Traits between Woody and Herbaceous Plants
Authors: Chen Haoxuan , Chen Shuang , Wang Xiaochun , Liu Xinrui , Wang Xue , Zhu Rong , Mo Weiyi , Wang Ruili , Zhang Shuoxin
Published: December 2022

Leaf nutrient resorption traits are regarded as important indicators reflecting the strategy of plant nutrient conservation, yet the mechanism underlying the variation of resorption traits in different plant growth forms (PGFs) remains unclear. In order to untangle the phylogenetic and environmental influences on leaf nitrogen (N) and phosphorus (P) resorption traits between woody and herbaceous plants, we investigated N and P contents of green and senesced leaves in 53 species along an altitudinal gradient (1374–3649 m) in the Taibai Mountain of central China and estimated leaf N and P resorption efficiency and proficiency. Our results show that leaf N and P resorption efficiency (NRE and PRE) had significant positive trends with altitude in both woody and herbaceous plants (all p < 0.05); however, their altitudinal patterns of N and P resorption proficiency (NRP and PRP) were different. For woody plants, leaf NRP and NRE:PRE first decreased and then increased with altitude (p < 0.05), while NRP:PRP had the opposite trend (p < 0.05). In herbaceous plants, leaf NRP and PRP decreased but NRP:PRP increased with altitude (p < 0.05). Climatic factors exerted the major influences on the variation in leaf NRE and PRE (18.5–24.8% explained variation). However, phylogenetic taxonomy mainly affected the variation of leaf PRP and NRP:PRP (45.2% and 41.4% explained variation) in all species, NRP:PRP in woody plants (37.8% explained variation), and NRE:PRE in herbaceous plants (49.7% explained variation). In addition, leaf NRP:PRP showed a significant phylogenetic signal (Blomberg’s p < 0.05). These results highlight the importance of taking PGFs and phylogenetic information into consideration when examining the interspecies variation in leaf resorption under environmental changes, which can advance our knowledge of plant nutrient utilization strategies in response to fluctuating environments and lay the groundwork for the development of complex element biogeochemical models.
Co-Expression of ZmVPP1 with ZmNAC111 Confers Robust Drought Resistance in Maize
Authors: Liu Shengxue , Liu Xiaohu , Zhang Xiaomin , Chang Shujie , Ma Chao , Qin Feng
Published: December 2022

Drought is a primary environmental factor limiting maize production globally. Although transferring a single gene to maize can enhance drought resistance, maize response to water deficit requires further improvement to accommodate the steadily intensifying drought events worldwide. Here, we generated dual transgene lines simultaneously overexpressing two drought-resistant genes, ZmVPP1 (encoding a vacuolar-type H+ pyrophosphatase) and ZmNAC111 (encoding a NAM, ATAF, and CUC (NAC)-type transcription factor). Following drought stress, survival rates of the pyramided transgenic seedlings reached 62–66%, while wild-type and single transgene seedling survival rates were 23% and 37–42%, respectively. Maize seedlings co-expressing ZmVPP1 and ZmNAC111 exhibited higher photosynthesis rates, antioxidant enzyme activities, and root-shoot ratios than the wild type, and anthesis-silking intervals were shorter while grain yields were higher under water deficit conditions in field trials. Additionally, RNA-sequencing analysis confirmed that photosynthesis and stress-related metabolic processes were stimulated in the dual transgene plants under drought conditions. The findings in this work illustrate how high co-expression of different drought-related genes can reinforce drought resistance over that of individual transgene lines, providing a path for developing arid climate-adapted elite maize varieties.
Polymorphisms in COMT and OPRM1 Collectively Contribute to Chronic Shoulder Pain and Disability in South African Breast Cancer Survivors’
Authors: Firfirey Firzana , Shamley Delva , September Alison V.
Published: December 2022

Chronic shoulder pain and disability is a common adverse effect experienced by >40% of breast cancer survivors (BCS). Pain management protocols for acute and chronic pain include the use of opioids and opioid derivatives. Furthermore, pain-modulating genes, such as COMT and OPRM1, have been linked to the aetiology of chronic pain. This study aimed to investigate the association between genetic variants of major pain modulator genes and chronic pain/disability in BCS. Assessment of pain, disability and combined (pain and disability) symptoms were determined using the Shoulder Pain and Disability Index (SPADI). Participants were grouped according to their scores such as no-low (<30%) and moderate-high (≥30%) groups of pain, disability and combined (pain and disability). Genotyping of the COMT rs6269 (A > G), rs4633 (C > T), rs4818 (C > G) and the functional rs4680(G > A) SNPs within the BCS (N = 252) cohort were conducted using TaqMan® SNP assays. Genotype, allele, haplotype, and allele–allele combination frequencies were evaluated. Statistical analysis was applied, with significance accepted at p < 0.05. The COMT rs4680:A/A genotype was significantly associated with moderate-high pain (p = 0.024, OR: 3.23, 95% CI: 1.33–7.81) and combined (pain and disability) (p = 0.015, OR: 3.81, 95% CI: 1.47–9.85). The rs4680:A allele was also significantly associated with moderate-high pain (p = 0.035, OR: 1.58, 95% CI: 1.03–2.43) and combined (pain and disability) (p = 0.017, OR: 1.71, 95% CI: 1.07–2.71). For the inferred COMT (rs6269 A > G-rs4680 G > A) haplotype analyses, the G-G (p = 0.026, OR: 0.67, 95% CI: 0.38–1.18) and A-A (p = 0.007, OR: 2.09, 95% CI: 0.89–4.88) haplotypes were significantly associated with reduced and increased likelihoods of reporting moderate-high pain, respectively. The inferred A-A (p = 0.003, OR: 2.18, 95% CI: 0.92–5.17) haplotype was also significantly associated with combined (pain and disability). Gene–gene interaction analyses further showed allele–allele combinations for COMT (rs4680 G > A)-OPRM1 (rs1799971 A > G) and COMT (rs4680 G > A)-OPRM1(rs540825 T > A) were associated with reporting pain and combined (pain and disability) symptoms, p < 0.05. The findings of this study suggest that COMT and OPRM1 SNPs play a role in the development of chronic shoulder pain/disability in BCS in a unique South African cohort from the Western Cape.
Harnessing on Genetic Variability and Diversity of Rice (Oryza sativa L.) Genotypes Based on Quantitative and Qualitative Traits for Desirable Crossing Materials
Authors: Debsharma Sanjoy K. , Syed Md. Abu , Ali Md. Hannan , Maniruzzaman Sheikh , Roy Popy R. , Brestic Marian , Gaber Ahmed , Hossain Akbar
Published: December 2022

Yield is a complex parameter of rice due to its polygonal nature, sometimes making it difficult to coat the selection process in the breeding program. In the current study, 34 elite rice genotypes were assessed to evaluate 3 locations for the selection of desirable rice cultivars suitable for multiple environments based on genetic diversity. In variance analysis, all genotypes have revealed significant variations (p ≤ 0.001) for all studied characters, signifying a broader sense of genetic variability for selection purposes. The higher phenotypic coefficient of variation (PCV) and genotypic coefficient of variation (GCV) were found for yield-associated characteristics such as the number of grains panicle−1 (GP), panicles hill−1 (PPH), and tillers hill−1 (TILL). All of the characters had higher heritability (greater than 60%) and higher genetic advance (greater than 20%), which pointed out non-additive gene action and suggested that selection would be effective. The most significant traits causing the genotype variants were identified via principal component analysis. In the findings of the cluster analysis, 34 elite lines were separated into 3 categories of clusters, with cluster II being chosen as the best one. The relationship matrix between each elite cultivar and traits was also determined utilizing a heatmap. Based on multi-trait genotype-ideotype distance index (MGIDI), genotypes Gen2, Gen4, Gen14, Gen22, and Gen30 in Satkhira; Gen2, Gen6, Gen7, Gen15, and Gen30 in Kushtia; and Gen10, Gen12, Gen26, Gen30, and Gen34 in Barishal were found to be the most promising genotypes. Upon validation, these genotypes can be suggested for commercial release or used as potential breeding material in crossing programs for the development of cultivars suitable for multiple environments under the future changing climate.
ROS Homeostasis Involved in Dose-Dependent Responses of Arabidopsis Seedlings to Copper Toxicity
Authors: Wang Jiehua , Moeen-ud-din Muhammad , Yin Rong , Yang Shaohui
Published: December 2022

As an essential element in plant nutrition, copper (Cu) can promote or inhibit plant growth depending on its concentration. However, the dose-dependent effects of copper, particularly on DNA damage associated with reactive oxygen species (ROS) homeostasis, are much less understood. In this work, we analyzed the dual effect of Cu (5, 20, and 60 μM) on the reproductive performance of Arabidopsis plants. Whereas Cu5 promoted inflorescence initiation and increased kilo seed weight, two higher concentrations, Cu20 and Cu60, delayed inflorescence initiation and negatively affected silique size. Excess Cu also induced changes in cellular redox homeostasis, which was examined by in situ visualization and measurements of ROS, including superoxide (O2•−), hydrogen peroxide (H2O2), malonyldialdehyde (MDA), and plasma membrane damage. The most dramatic increases in the production of O2•− and H2O2 along with increased activity of superoxide dismutase (SOD) and glutathione peroxidase (GPX) and decreased activity of catalase (CAT) and ascorbate peroxidase (APX) were observed in roots with Cu60. Oxidative stress also modulated the expression levels of a number of genes involved in the DNA damage response (DDR), particularly those related to DNA repair. The Cu-induced chlorosis of Arabidopsis seedlings could be alleviated by exogenous addition of glutathione (GSH) and ascorbate (Asc), as the chlorophyll content was significantly increased. Overall, internal homeostasis ROS and the associated DDR pathway and the corresponding scavenging mechanisms play a central role in the response of Arabidopsis to oxidative stress induced by inhibitory Cu concentrations. Our results have shown, for the first time, that the biphasic responses of Arabidopsis seedlings to increasing Cu concentrations involve different DNA damage responses and oxidative reactions. They provide the basis for elucidating the network of Cu-induced DDR-related genes and the regulatory mechanism of the complex ROS production and scavenging system.
The Role of miRNAs, circRNAs and Their Interactions in Development and Progression of Hepatocellular Carcinoma: An Insilico Approach
Authors: Ishaq Yasmeen , Ikram Aqsa , Alzahrani Badr , Khurshid Sana
Published: December 2022

Hepatocellular carcinoma (HCC) is a type of malignant tumor. miRNAs are noncoding RNAs and their differential expression patterns are observed in HCC-induced by alcoholism, HBV and HCV infections. By acting as a competing endogenous RNA (ceRNA), circRNA regulates the miRNA function, indirectly controlling the gene expression and leading to HCC progression. In the present study, data mining was performed to screen out all miRNAs and circRNA involved in alcohol, HBV or HCV-induced HCC with statistically significant (≤0.05%) expression levels reported in various studies. Further, the interaction of miRNAs and circRNA was also investigated to explore their role in HCC due to various causative agents. Together, these study data provide a deeper understanding of the circRNA–miRNA regulatory mechanisms in HCC. These screened circRNA, miRNA and their interactions can be used as prognostic biomarkers or therapeutic targets for the treatment of HCC.
Cross-Trait Genetic Analyses Indicate Pleiotropy and Complex Causal Relationships between Headache and Thyroid Function Traits
Authors: Tasnim Sana , Wilson Scott G. , Walsh John P. , Nyholt Dale R.
Published: December 2022

Epidemiological studies have reported a comorbid relationship between headache and thyroid traits; however, little is known about the shared genetics and causality that contributes to this association. We investigated the genetic overlap and associations between headache and thyroid function traits using genome-wide association study (GWAS) data. We found a significant genetic correlation (rg) with headache and hypothyroidism (rg = 0.09, p = 2.00 × 10−4), free thyroxine (fT4) (rg = 0.08, p = 5.50 × 10−3), and hyperthyroidism (rg = −0.14, p = 1.80 × 10−3), a near significant genetic correlation with secondary hypothyroidism (rg = 0.20, p = 5.24 × 10−2), but not with thyroid stimulating hormone (TSH). Pairwise-GWAS analysis revealed six, 14, four and five shared (pleiotropic) loci with headache and hypothyroidism, hyperthyroidism, secondary hypothyroidism, and fT4, respectively. Cross-trait GWAS meta-analysis identified novel genome-wide significant loci for headache: five with hypothyroidism, three with secondary hypothyroidism, 12 with TSH, and nine with fT4. Of the genes at these loci, six (FAF1, TMX2-CTNND1, AARSD1, PLCD3, ZNF652, and C20orf203; headache-TSH) and six (HMGB1P45, RPL30P1, ZNF462, TMX2-CTNND1, ITPK1, SECISBP2L; headache-fT4) were significant in our gene-based analysis (pFisher’s combined p-value < 2.09 × 10−6). Our causal analysis suggested a positive causal relationship between headache and secondary hypothyroidism (p = 3.64 × 10−4). The results also suggest a positive causal relationship between hypothyroidism and headache (p = 2.45 × 10−3) and a negative causal relationship between hyperthyroidism and headache (p = 1.16 × 10−13). These findings suggest a strong evidence base for a genetic correlation and complex causal relationships between headache and thyroid traits.
Geophysical and Geochemical Proxies of Neolithic Sites from Thessaly: A Comparative Study on the Potential of Soil Magnetic Susceptibility and Phosphate Analyses for Minimally Invasive Location and Interpretation of Buried Features
Authors: Cuenca-García Carmen , Aidona Elina , Wilson Clare , Jrad Abir , Sarris Apostolos
Published: December 2022

This paper presents the results of a study exploring the potential of magnetic-susceptibility and phosphate soil analyses to locate and characterize buried Neolithic settlements in Thessaly, Greece. Using the preliminary results of large-area magnetometer surveys, soil samples were collected at three well-known sites along exploratory lines and augers targeting the locations of possible features of interest, including habitational structures and enclosures. The results demonstrated the capability of these analyses to detect the sites, outline hotspots and better interpret the features identified in the magnetometer results.