bluuebunny/crossref_metadata_2025 huggingface dataset:
Androgen and Estrogen Receptor Expression in Different Types of Perianal Gland Tumors in Male Dogs
Authors: Brodzki Adam , Łopuszyński Wojciech , Millan Yolanda , Tatara Marcin R. , Brodzki Piotr , Kulpa Katarzyna , Minakow Natalia
Published: March 2021

Perianal gland tumors are modified sebaceous glands present in the skin of the perianal region in the dog. Hormonal stimulation may induce hyperplasia of the perianal glands or their neoplastic progression. The presence of androgen (AR) and estrogen (ER) receptors have been demonstrated both in normal perianal glands as well as in perianal tumors. The aim of the study was an immunohistochemical assessment of the expression of estrogen and androgen receptors in perianal gland tumors in dogs as an applicatory marker for antihormonal treatment. Biopsy samples of perianal masses were collected from 41 male dogs. A histopathological examination revealed 24 adenomas, 12 epitheliomas and five carcinomas. The immunohistochemical staining showed a mainly nuclear expression of AR and ER in the neoplastic cells. Both the androgen and estrogen receptors were expressed in adenoma, epithelioma and carcinoma cases; however, the highest expression of the receptors was stated in the adenoma and epithelioma. In the case of the carcinoma, the expression of sex hormone receptors was very weak. The differences of the number of cells expressing AR and ER as well as the observed differentiated intensity of staining in the studies demonstrated that the determination of the expression of the sex hormone receptors may be useful to elaborate a diagnostic and therapeutic algorithm.
Mammary Gland Structures Are Not Affected by an Increased Growth Rate of Yearling Ewes Post-Weaning but Are Associated with Growth Rates of Singletons
Authors: Haslin Emmanuelle , Corner-Thomas Rene A. , Kenyon Paul R. , Molenaar Adrian J. , Morris Stephen T. , Blair Hugh T.
Published: March 2021

The experiment aimed to examine the impacts of an increased growth rate of ewes between three and seven months of age on udder development using ultrasound and to establish whether ultrasonography could be used to identify ewe mammary structures that may be indirect indicators of singleton growth to weaning. Udder dimensions, depths of gland cistern (GC), parenchyma (PAR) and fat pad (FP) were measured in late pregnancy (P107), early lactation (L29), and at weaning (L100) in 59 single-bearing yearling ewes selected from two treatments. The ‘heavy’ group (n = 31) was preferentially fed prior to breeding achieving an average breeding live-weight of 47.9 ± 0.38 kg at seven months of age. The ‘control’ group (n = 28) had an average breeding live-weight of 44.9 ± 0.49 kg. Udder dimensions, GC, PAR and FP did not differ between treatments. Lamb growth to L100 was positively associated (p < 0.05) with PAR at P107 and GC at L29. There was no evidence of negative effects of the live-weight gain treatments on udder development of yearling ewes as measured by ultrasonography. The results suggest that this ultrasound method has the potential to identify pregnant yearling ewes which would wean heavier singletons.
Use of Spinal Anaesthesia with Anaesthetic Block of Intercostal Nerves Compared to a Continuous Infusion of Sufentanyl to Improve Analgesia in Cats Undergoing Unilateral Mastectomy
Authors: Cicirelli Vincenzo , Debidda Pasquale , Maggio Nicola , Caira Michele , Mrenoshki Daniela , Aiudi Giulio G. , Lacalandra Giovanni M.
Published: March 2021

Unilateral mastectomy is a common surgical procedure in feline species and requires postoperative pain management. Our study aimed to evaluate the analgesic efficacy of subarachnoid anaesthesia combined with an intercostal nerve block, in comparison with the use of sufentanyl citrate administered as a constant-rate infusion (CRI). Twenty cats were randomly divided into two groups (n = 10/group) based on the analgesic protocol used: the first received loco-regional anaesthesia with levobupivacaine (LR group), and the second received a CRI of sufentanyl (SUF group). The evaluation criteria during surgery were the need for a bolus of fentanyl in the event of an increased heart rate or increased blood pressure. In the postoperative period, the levels of comfort/discomfort and pain were used to obtain a score according to the UNESP-Botucatu multimodal scale. Subjects who scored above seven received analgesic drug supplementation. Intraoperative analgesia was satisfactory, with good haemodynamic stability in both groups. Four patients in the LR group required an extra dose of methadone after they achieved the sternal decubitus position, whereas those in the SUF group required many more doses. The analgesia achieved in the LR group was more satisfactory than that in the SUF group.
Differential Rotational Movement of the Thoracolumbosacral Spine in High-Level Dressage Horses Ridden in a Straight Line, in Sitting Trot and Seated Canter Compared to In-Hand Trot
Authors: MacKechnie-Guire Russell , Pfau Thilo
Published: March 2021

Assessing back dysfunction is a key part of the investigative process of “loss of athletic performance” in the horse and quantitative data may help veterinary decision making. Ranges of motion of differential translational and rotational movement between adjacent inertial measurement units attached to the skin over thoracic vertebrae 5, 13 and 18 (T5, T13, T18) lumbar vertebra 3 (L3) and tuber sacrale (TS) were measured in 10 dressage horses during trot in-hand and ridden in sitting trot/canter. Straight-line motion cycles were analysed using a general linear model (random factor: horse; fixed factor: exercise condition; Bonferroni post hoc correction: p < 0.05). At T5-T13 the differential heading was smaller in sitting trot (p ≤ 0.0001, 5.1° (0.2)) and canter (p ≤ 0.0001, 3.2° (0.2)) compared to trotting in-hand (7.4° (0.4)). Compared to trotting in-hand (3.4° (0.4)) at T18-L3 differential pitch was higher in sitting trot (p ≤ 0.0001, 7.5° (0.3)) and canter (p ≤ 0.0001, 6.3° (0.3)). At L3-TS, differential pitch was increased in canter (6.5° (0.5)) compared to trotting in-hand (p = 0.006, 4.9° (0.6)) and differential heading was higher in sitting trot (4° (0.2)) compared to canter (p = 0.02, 2.9° (0.3)). Compared to in-hand, reduced heading was measured in the cranial–thoracic area and increased in the caudal–thoracic and lumbar area. Pitch increased with ridden exercise from the caudal–thoracic to the sacral area.
Socialization in Commercial Breeding Kennels: The Use of Novel Stimuli to Measure Social and Non-Social Fear in Dogs
Authors: Pritchett Margaret , Barnard Shanis , Croney Candace
Published: March 2021

Understanding the behavioral welfare of dogs in commercial breeding kennels (CBKs) is important for improving breeders’ management practices as well as dog welfare. In the current study, breeding dogs from CBKs were exposed to novel stimuli to evaluate their behavioral responses, with emphasis on indicators of fear. Subjects were presented with a standard stranger-approach test, a traffic cone, and a realistic dog statue. Sixty dogs were exposed to the three stimuli and behavioral responses were scored using an ethogram developed for this study. Dogs spent significantly more time investigating the environment, staying further away from the stimulus, and they took longer to approach and investigate when presented with the cone than with the dog statue or stranger (p < 0.01). These findings suggest that the cone elicited more fear-related behaviors than the dog statue and stranger. Given these results, in addition to socializing their dogs to unfamiliar people and other dogs within their kennels, commercial breeders should be encouraged to increase the exposure of their dogs to more diverse novel stimuli to reduce non-social fear and support the welfare of dogs while they reside in the kennel and when they transition to new homes.
Steatitis in Cold-Stunned Kemp’s Ridley Sea Turtles (Lepidochelys kempii)
Authors: Turner Rachel , Innis Charles , Stacy Brian , Hernandez Jorge , Hill Richard , Scott Karen , Frasca Salvatore , Garner Michael , Burns Rachel , Arendt Michael , Brisson Jennifer , Norton Terry , Williams Sea , Kennedy Adam , Alexander Amy , Stacy Nicole
Published: March 2021

The pathogenesis of steatitis that infrequently occurs in cold-stunned Kemp’s ridley sea turtles (KRT; Lepidochelys kempii) has been undetermined. The objectives of this study were to investigate the clinical (n = 23) and histologic findings (n = 11) in cold-stunned KRT, and to compare plasma concentrations of α-tocopherol (vitamin E), thiobarbituric acid reactive substances (TBARS), and the TBARS to vitamin E (T/E) ratio (an assessment of oxidative stress) between cold-stunned KRT with clinically and/or histologically confirmed steatitis (n = 10) and free-ranging KRT (n = 9). None of the cold-stunned turtles had clinically detectable steatitis at admission, and the median number of days to diagnosis of steatitis was 71 (range 33–469). Histologic findings of affected adipose tissue included heterophilic (n = 9) and/or histiocytic (n = 5) steatitis, fat necrosis (n = 7), myonecrosis (n = 2), and intralesional bacteria (n = 6). Cold-stunned KRT had significantly lower plasma vitamin E concentrations (median = 3.5 nmol/g), lower plasma TBARS concentrations (median = 1.6 nmol/g), and higher T/E ratios (median = 0.50), than controls (62.3 nmol/g; 2.1 nmol/g; 0.03, respectively). These results suggest a multifactorial etiology for the development of steatitis in KRT during rehabilitation, including tissue injury, septicemia, and various factors resulting in imbalances of anti-/oxidative status. By highlighting the need to provide more effective vitamin E supplementation, and the need to re-assess specific components of the diet, this study may lead to reduced incidence and improved medical management of steatitis in cold-stunned sea turtles.
Design and Implementation of Poultry Farming Information Management System Based on Cloud Database
Authors: Zheng Haikun , Zhang Tiemin , Fang Cheng , Zeng Jiayuan , Yang Xiuli
Published: March 2021

Aiming at breaking down the bottleneck problems of different scale of poultry farms, the low profitability of poultry farming, and backward information management in China, a safe and efficient information management system for poultry farming was designed. This system consists of (1) a management system application layer, (2) a data service layer, and (3) an information sensing layer. The information sensing layer obtains and uploads production and farming information through the wireless sensor network built in the poultry house. The use of a cloud database as an information storage carrier in the data service layer eliminates the complex status of deploying local server clusters, and it improves the flexibility and scalability of the system. The management system application layer contains many sub-function modules including poultry disease detection functions to realize the visual management of farming information and health farming; each module operates independently and cooperates with each other to form a set of information management system for poultry farming with wide functional coverage, high service efficiency, safety, and convenience. The system prototype has been tested for the performance of wireless sensor network and cloud database, and the results show that the prototype is capable of acquiring and managing poultry farming information.
Effect of Season and Social Environment on Semen Quality and Endocrine Profiles of Three Endangered Ungulates (Gazella cuvieri, G. dorcas and Nanger dama)
Authors: Arregui Lucía , Garde José Julián , Soler Ana Josefa , Espeso Gerardo , Roldan Eduardo R. S.
Published: March 2021

Knowledge of factors affecting semen quality could be of great importance for the collection and preservation of semen from threatened animals. To assess the effect of seasonality, sperm parameters and testosterone levels were examined throughout the year and compared with the distribution of conceptions. Cuvier’s gazelle showed higher sperm quantity in April, coinciding with one peak of conceptions. In dorcas gazelle, sperm parameters showed a drop in October. However, percentage of conceptions increased during that month. In Mohor gazelle, sperm quality was best in April and August, in agreement with higher conception rates and high testosterone levels. Percentage of conceptions was correlated with photoperiod and rainfall in Cuvier’s gazelle and with temperature in Mohor gazelle. To assess the effect of social environment, semen quality, testosterone and cortisol levels were quantified in males housed alone, in bachelor groups or with females. No differences were seen in Cuvier’s and Mohor gazelles’ semen traits, whereas dorcas males housed with females showed lower semen quality than males kept alone or with other males. Overall, ejaculate quality is influenced by seasonal factors in the three gazelle species, while social factors only appear to affect that of dorcas gazelle.
Effects of L-Glutamine Supplementation during the Gestation of Gilts and Sows on the Offspring Development in a Traditional Swine Breed
Authors: Vázquez-Gómez Marta , García-Contreras Consolación , Astiz Susana , Torres-Rovira Laura , Pesantez-Pacheco José Luis , Heras-Molina Ana , Castro Madrigal Teresa , López-Bote Clemente , Óvilo Cristina , González-Bulnes Antonio , Isabel Beatriz
Published: March 2021

The use of amino acids during pregnancy, such as glutamine (Gln), seems to be a promising strategy in selected swine breeds to improve the offspring prenatal development. The main goal of the current study was to assess the development of the offspring from parity 1–3 sows of a traditional breed, which were supplemented with 1% glutamine after Day 35 of gestation, under farm conditions. A total of 486 (288 treated) piglets from 78 (46 treated) Iberian sows were used. At birth and slaughterhouse, fatty acid composition, metabolism, and mTOR pathway gene expression were analyzed. At birth, treated newborns showed greater amounts of specific amino acids in plasma, such as glutamine, asparagine, or alanine, and Σn-3 fatty acids in cellular membranes than control newborns. The expression of genes belonging to mTOR Complex 1 was also higher in treated piglets with normal birth-weight. However, these findings did not improve productive traits at birth or following periods in litters from supplemented gilts (parity 1) or sows (parities 2–3). Thus, further research is needed to properly understand the effects of prenatal glutamine supplementation, particularly in traditional swine breeds.
Whole-Genome Sequencing and Characterization of Buffalo Genetic Resources: Recent Advances and Future Challenges
Authors: Rehman Saif ur , Hassan Faiz-ul , Luo Xier , Li Zhipeng , Liu Qingyou
Published: March 2021

The buffalo was domesticated around 3000–6000 years ago and has substantial economic significance as a meat, dairy, and draught animal. The buffalo has remained underutilized in terms of the development of a well-annotated and assembled reference genome de novo. It is mandatory to explore the genetic architecture of a species to understand the biology that helps to manage its genetic variability, which is ultimately used for selective breeding and genomic selection. Morphological and molecular data have revealed that the swamp buffalo population has strong geographical genomic diversity with low gene flow but strong phenotypic consistency, while the river buffalo population has higher phenotypic diversity with a weak phylogeographic structure. The availability of recent high-quality reference genome and genotyping marker panels has invigorated many genome-based studies on evolutionary history, genetic diversity, functional elements, and performance traits. The increasing molecular knowledge syndicate with selective breeding should pave the way for genetic improvement in the climatic resilience, disease resistance, and production performance of water buffalo populations globally.
Is Harbor Porpoise (Phocoena phocoena) Exhaled Breath Sampling Suitable for Hormonal Assessments?
Authors: Reckendorf Anja , Schmicke Marion , Bunskoek Paulien , Anderson Hansen Kirstin , Thybo Mette , Strube Christina , Siebert Ursula
Published: March 2021

Over the last decades, exhaled breath sampling has been established for laboratory analysis in various cetacean species. Due to their small size, the usability of respiratory vapor for hormone assessments was questionable in harbor porpoises (Phocoena phocoena). This pilot study compared three different blow collection devices for their suitability in the field and during laboratory processing: a sterile petri dish covered by a Nitex membrane, as well as sterile 50 mL centrifuge tubes with or without manganese(II) chloride as a stabilizer. Collected exhales varied between three, five or ten, depending on feasibility. Hormones were extracted through an ether mix, followed by centrifugal evaporation and cortisol analysis using an immunoassay. Although close to the lower end of the assay’s dynamic range, the ELISA produced results (n = 110, 0.102–0.937 ng/mL). Hence, a simple 50 mL centrifuge tube was determined as the best suited blow collection device, while three consecutive exhales proved sufficient to yield results. These findings are promising regarding the suitability of exhaled breath as a matrix for future endocrine and immune system-related studies in harbor porpoises. If further advanced, blow sampling can become an important, non-invasive tool for studying and monitoring health, stress levels and diseases in harbor porpoises.
Lunar Phases and Wildlife–Vehicle Collisions: Application of the Lunar Disk Percentage Method
Authors: Ignatavičius Gytautas , Ulevičius Alius , Valskys Vaidotas , Galinskaitė Lina , Busher Peter E. , Trakimas Giedrius
Published: March 2021

We investigated the relationship between lunar illumination based on the percentage of the visible lunar disk (LDP) and the frequency of wildlife–vehicle collisions (WVCs) in Lithuania. We analyzed WVC frequency during ten 10% LDP intervals to more precisely reflect the relationship between LDP and WVC. The 10% LDP interval approach showed a significant trend of increasing WVC frequencies with an increasing LDP at night. We also examined the correlation between the daily numbers of WVCs and LDP for different months and seasons. The relationship seemed to be stronger at night and during the late autumn–winter months, particularly in December, suggesting the importance of lunar illumination on WVCs. There was a weak positive correlation between LDP and overall daily number of WVCs (rs = 0.091; p < 0.001) and between LDP and night WVCs (rs = 0.104; p < 0.001). We found significant positive correlations for winter (December–February) (rs = 0.118; p = 0.012) and autumn (August–November) (rs = 0.127; p = 0.007). Our study suggests that the LDP interval approach may provide more possibilities for the evaluation and quantification of WVCs and lunar light relationships than the traditional lunar phase approach.
Pain, Pathophysiological Mechanisms, and New Therapeutic Options for Alternative Analgesic Agents in Sheep: A Review and Investigation
Authors: Kania Bogdan Feliks , Wrońska Danuta , Bracha Urszula
Published: March 2021

Relief from suffering is the guiding principle of medical and veterinary ethics. Medical care for animals should be carried out to meet all welfare conditions. The need for pain management is demonstrated by recent monographs devoting attention to this urgent ethical need. Little data, however, are available on the prevention and attenuation of pain in sheep. After administration of narcotic analgesics used for severe visceral pain, sheep react with a state of excitement. Therefore, it was decided to experimentally investigate the usefulness of potential non-narcotic drugs to relieve pain in sheep with intestinal colic caused by 10 min of mechanical distension of their duodenal and/or descending colonic wall. The results indicate the potential usefulness of VGCCIs (diltiazem, nifedipine, verapamil), cholecystokinin receptor antagonists (PD, proglumide), and metabotropic glutaminergic receptor antagonists (mGluRAs), such as L-AP3, DL-AP3. As a premedication, these substances prevented the occurrence of symptoms of acute intestinal pain including atony of reticulo-rumen, tachycardia, hyperventilation, moaning, gnashing of teeth, hypercortisolemia, and catecholaminemia; hence, these substances are considered potential agents in the treatment of sheep visceral pain.
A Model for Dental Age Verification Using Ultrastructural Imaging for Modern and Fossil Representatives of the Rhinocerotidae Family
Authors: Pasicka Edyta , Nowakowski Dariusz , Bendrey Robin , Melnyk Oleg P.
Published: March 2021

The analyses were performed on a right third premolar (P3) of a white rhinoceros female (Ceratotherium simum, Burchell 1817). The specimen was born in captivity at London Zoo (Zoological Society of London), then in the 1970s transferred to Kiev Zoo (Peremohy Avenue), Ukraine, and was kept there until it died at a documented chronological age of 48 years. The female died because of its age, which indicates it was kept in good conditions adequate to the requirements of this species. Photographs and micrographs with radiological documentation were taken on the said tooth. Its structural characteristics were determined, and on the occlusal surface areas and points of anatomical constitution of its crown were identified. The tooth was also histologically evaluated via sections taken horizontally in a mesial-distal plane through the crown, horizontally in a mesial-distal plane through the coronal portion of the root, and longitudinally in a lingual-buccal plane through the crown and the root. Preparations with ground sections were made and observed in white, polarized, and reflected light. In the subsequent stage X-ray and SEM imaging has also been used, for analysis of the distribution of annual growth layers of mineralized dental tissues of cement and dentine, counted from the root canal center to the buccal surface. An attempt was also made to confirm the annual season in which the animal died, based on cement growth lines. It was observed that the growth lines were visible in all the analyzed sections, in dentine and cement. In the cement, the lines were relatively few and did not represent the attested age of the animal. The analysis of the coloration of the cement lines indicated that the animal was regularly fed a diet that was not seasonally differentiated. From the X-ray examination comes a conclusion that the animal did not suffer from periodontal diseases. Visible growth lines were observed on the dentine. On the horizontal section through the crown growth lines in the dentine were few and unclear. On the longitudinal section, both on the caudal and rostral roots, these lines were clearly visible and much more numerous than expected considering the known age of the animal, as more than 50 were counted. On horizontal sections through the upper part of both roots, distinct growth lines were observed in the dentine, and their number—48 for both roots—corresponded precisely to the age of the animal. The results of our study indicate that this method has significant potential for application to verify the age at death for modern and fossil representatives of rhinoceros.
Pulse Oximetry as a Diagnostic Tool to Determine Pulp Vitality: A Systematic Review
Authors: Almudever-Garcia Alicia , Forner Leopoldo , Sanz José Luis , Llena Carmen , Rodríguez-Lozano Francisco Javier , Guerrero-Gironés Julia , Melo María
Published: March 2021

Pulse oximetry (PO) can be used as a technique to assess vascularization. Although it is commonly used in the field of medicine, it still has limited use in dentistry. The aim of this study is to evaluate, by means of a systematic review, if pulse oximetry is a viable and effective system to determine pulp vitality and assess the clinical criteria for its use. A literature search was performed in Medline, Scopus, Embase, and Web of Science databases. Clinical studies using pulse oximetry as a tool to determine pulp vitality in permanent teeth were eligible. Quality assessment of the included studies was performed following the Quality Assessment for Diagnostic Accuracy Studies (QUADAS-2) guidelines. A total of 24 articles were included in the review. The subjective test most compared to PO was the cold test. PO has shown a good sensibility and specificity, and it has been used in different clinical situations. Pulse oximetry has a high potential as a tool for the diagnosis of pulp vitality. However, the absence of a specific pulse oximeter for a dental practice, among other limitations, added to the lack of evidence on how different systemic and oral pathologies may affect the pulp’s oxygen saturation levels, which hinders its clinical use.
A General Framework Based on Machine Learning for Algorithm Selection in Constraint Satisfaction Problems
Authors: Ortiz-Bayliss José C. , Amaya Ivan , Cruz-Duarte Jorge M. , Gutierrez-Rodriguez Andres E. , Conant-Pablos Santiago E. , Terashima-Marín Hugo
Published: March 2021

Many of the works conducted on algorithm selection strategies—methods that choose a suitable solving method for a particular problem—start from scratch since only a few investigations on reusable components of such methods are found in the literature. Additionally, researchers might unintentionally omit some implementation details when documenting the algorithm selection strategy. This makes it difficult for others to reproduce the behavior obtained by such an approach. To address these problems, we propose to rely on existing techniques from the Machine Learning realm to speed-up the generation of algorithm selection strategies while improving the modularity and reproducibility of the research. The proposed solution model is implemented on a domain-independent Machine Learning module that executes the core mechanism of the algorithm selection task. The algorithm selection strategies produced in this work are implemented and tested rapidly compared against the time it would take to build a similar approach from scratch. We produce four novel algorithm selectors based on Machine Learning for constraint satisfaction problems to verify our approach. Our data suggest that these algorithms outperform the best performing algorithm on a set of test instances. For example, the algorithm selectors Multiclass Neural Network (MNN) and Multiclass Logistic Regression (MLR), powered by a neural network and linear regression, respectively, reduced the search cost (in terms of consistency checks) of the best performing heuristic (KAPPA), on average, by 49% for the instances considered for this work.
Recent Advancements in Non-Destructive Testing Techniques for Structural Health Monitoring
Authors: Kot Patryk , Muradov Magomed , Gkantou Michaela , Kamaris George S. , Hashim Khalid , Yeboah David
Published: March 2021

Structural health monitoring (SHM) is an important aspect of the assessment of various structures and infrastructure, which involves inspection, monitoring, and maintenance to support economics, quality of life and sustainability in civil engineering. Currently, research has been conducted in order to develop non-destructive techniques for SHM to extend the lifespan of monitored structures. This paper will review and summarize the recent advancements in non-destructive testing techniques, namely, sweep frequency approach, ground penetrating radar, infrared technique, fiber optics sensors, camera-based methods, laser scanner techniques, acoustic emission and ultrasonic techniques. Although some of the techniques are widely and successfully utilized in civil engineering, there are still challenges that researchers are addressing. One of the common challenges within the techniques is interpretation, analysis and automation of obtained data, which requires highly skilled and specialized experts. Therefore, researchers are investigating and applying artificial intelligence, namely machine learning algorithms to address the challenges. In addition, researchers have combined multiple techniques in order to improve accuracy and acquire additional parameters to enhance the measurement processes. This study mainly focuses on the scope and recent advancements of the Non-destructive Testing (NDT) application for SHM of concrete, masonry, timber and steel structures.
Deposition of 3YSZ-TiC PVD Coatings with High-Power Impulse Magnetron Sputtering (HiPIMS)
Authors: Gaedike Bastian , Guth Svenja , Kern Frank , Killinger Andreas , Gadow Rainer
Published: March 2021

Optimized coating adhesion and strength are the advantages of high-power impulse magnetron sputtering (HiPIMS) as an innovative physical vapor deposition (PVD) process. When depositing electrically non-conductive oxide ceramics as coatings with HiPIMS without dual magnetron sputtering (DMS) or mid-frequency (MF) sputtering, the growing coating leads to increasing electrical insulation of the anode. As a consequence, short circuits occur, and the process breaks down. This phenomenon is also known as the disappearing anode effect. In this study, a new approach involving adding electrically conductive carbide ceramics was tried to prevent the electrical insulation of the anode and thereby guarantee process stability. Yttria-stabilized zirconia (3YSZ) with 30 vol.% titanium carbide (TiC) targets are used in a non-reactive HiPIMS process. The main focus of this study is a parameter inquisition. Different HiPIMS parameters and their impact on the measured current at the substrate table are analyzed. This study shows the successful use of electrically conductive carbide ceramics in a non-conductive oxide as the target material. In addition, we discuss the observed high table currents with a low inert gas mix, where the process was not expected to be stable.
Gas Foil Bearing Technology Enhanced with Smart Materials
Authors: Martowicz Adam , Roemer Jakub , Kantor Sławomir , Zdziebko Paweł , Żywica Grzegorz , Bagiński Paweł
Published: March 2021

The paper discusses a perspective of the usage of various types of smart materials to enhance the operational properties of Gas Foil Bearings. The authors, referring to the current investigation on thermomechanical characteristics of the above-mentioned bearing type, have focused on the concept of using Shape Memory Alloys, Piezoelectric Transducers, Thermoelectric Modules and Thermocouples to improve both mechanical and thermal behavior of the bearings. Based on the available literature and the authors’ experience, the present work provides an overview of the known and perspective applications of smart materials to Gas Foil Bearings. In particular, a discussion on their capabilities, limitations and effectiveness is conducted, taking into account the unique characteristics and requirements of the studied type of bearings.
Trends and Challenges in Intelligent Condition Monitoring of Electrical Machines Using Machine Learning
Authors: Kudelina Karolina , Vaimann Toomas , Asad Bilal , Rassõlkin Anton , Kallaste Ants , Demidova Galina
Published: March 2021

A review of the fault diagnostic techniques based on machine is presented in this paper. As the world is moving towards industry 4.0 standards, the problems of limited computational power and available memory are decreasing day by day. A significant amount of data with a variety of faulty conditions of electrical machines working under different environments can be handled remotely using cloud computation. Moreover, the mathematical models of electrical machines can be utilized for the training of AI algorithms. This is true because the collection of big data is a challenging task for the industry and laboratory because of related limited resources. In this paper, some promising machine learning-based diagnostic techniques are presented in the perspective of their attributes.
ORC Optimal Design through Clusterization for Waste Heat Recovery in Anaerobic Digestion Plants
Authors: Frate Guido Francesco , Baccioli Andrea , Lucchesi Elena , Ferrari Lorenzo
Published: March 2021

Waste heat recovery (WHR) systems through organic rankine cycles (ORCs) in anaerobic digestion plants may improve cogeneration efficiency. Cogeneration unit power output, flue gas temperature, and mass flow rate are not constant during the day, and the thermal load requested by digesters shows seasonal variations. For this reason, a proper design of the ORC is required. In this study, a design methodology is proposed, based on the clustering of the boundary conditions expected during one year of operation and the anaerobic digestion plant operation. The design has to be a compromise between part-load operation and nominal power rating. In this study, the ORC design boundary conditions were partitioned into four representative clusters with a different population, and the centroid of each cluster was assumed as a potential representative boundary condition for the cycle design. Four different ORC designs, one for each cluster, were defined through an optimization problem that maximized the cycle net power output. ORC designs were compared to those resulting from the seasonal average boundary conditions. The comparison was made based on the ORC off-design performance. Part-load behavior was estimated by implementing a sliding-pressure control strategy and the annual production was therefore calculated. ORC off-design was studied through a detailed Aspen HYSYS simulation. Simulations showed that the power output of each design was directly connected to the cluster population. The design obtained from the most populated cluster generated 10% more energy than that from a system designed by taking into account only the year average conditions.
Metal-Organic Hybrid Metamaterials for Spectral-Band Selective Active Terahertz Modulators
Authors: Yoo Hyung Keun , Cho Soo Bin , Park Sae June , Ahn Yeong Hwan , Kang Chul , Hwang In-Wook , Lee Joong Wook
Published: March 2021

Optically controlled spectral-band selective terahertz (THz) modulators based on metal-organic hybrid metamaterials were investigated. An artificially structured material, which consists of two single split-ring resonators put together on the split gap side, was patterned on a silicon substrate to generate frequency-selective properties. An active layer of an organic thin film (fullerene derivative [6,6]-phenyl-C61-butyric acid methyl ester, also called PCBM) was deposited on the metamaterial-silicon structure for modulating the transmission of incident THz radiation. The metal-organic hybrid metamaterials enabled active control of spectral bands present in the transmission spectra of THz waves. In addition, the changes in the photo-excited carrier density due to the transfer of charges between the layers were quantitatively analyzed by simulation results.
Is Digital Twin Technology Supporting Safety Management? A Bibliometric and Systematic Review
Authors: Agnusdei Giulio Paolo , Elia Valerio , Gnoni Maria Grazia
Published: March 2021

In the Industry 4.0 era, digital tools applied to production and manufacturing activities represent a challenge for companies. Digital Twin (DT) technology is based on the integration of different “traditional” tools, such as simulation modeling and sensors, and is aimed at increasing process performance. In DTs, simulation modeling allows for the building of a digital copy of real processes, which is dynamically updated through data derived from smart objects based on sensor technologies. The use of DT within manufacturing activities is constantly increasing, as DTs are being applied in different areas, from the design phase to the operational ones. This study aims to analyze existing fields of applications of DTs for supporting safety management processes in order to evaluate the current state of the art. A bibliometric review was carried out through VOSviewer to evaluate studies and applications of DTs in the engineering and computer science areas and to identify research clusters and future trends. Next, a bibliometric and systematic review was carried out to deepen the relation between the DT approach and safety issues. The findings highlight that in recent years, DT applications have been tested and developed to support operators during normal and emergency conditions and to enhance their abilities to control safety levels.
Self-Assembled Multinuclear Complexes for Cobalt(II/III) Mediated Sensitized Solar Cells
Authors: Marchini Edoardo , Caramori Stefano , Boaretto Rita , Cristino Vito , Argazzi Roberto , Niorettini Alessandro , Bignozzi Carlo Alberto
Published: March 2021

In this work, we designed a tetranuclear self-assembled dye 4 (2Z907-Ag+-(Ru(TMAM))) exploiting a combination of the antenna effect and positively-charged groups designed to repel the oxidized form of cationic cobalt redox mediators, in order to reduce recombination and increase the efficiency of dye sensitized solar cells (DSSCs). Charge transfer and excited dynamics were probed by photoelectrochemical and photophysical measurements. The sensitized cell performance, recorded with a [Co(bpy)3]3+/2+ redox mediator and PEDOT counter electrode, showed an improvement when passing from Z907 to the multinuclear systems. The enhancement of the efficiency compared to Z907 resulted mainly from a superior steric and electrostatic shielding determined by the simultaneous presence of long alkyl chains and quaternary ammonia ion units in the architecture of 4.
Low-Light Photodetectors for Fluorescence Microscopy
Authors: Yokota Hiroaki , Fukasawa Atsuhito , Hirano Minako , Ide Toru
Published: March 2021

Over the years, fluorescence microscopy has evolved and has become a necessary element of life science studies. Microscopy has elucidated biological processes in live cells and organisms, and also enabled tracking of biomolecules in real time. Development of highly sensitive photodetectors and light sources, in addition to the evolution of various illumination methods and fluorophores, has helped microscopy acquire single-molecule fluorescence sensitivity, enabling single-molecule fluorescence imaging and detection. Low-light photodetectors used in microscopy are classified into two categories: point photodetectors and wide-field photodetectors. Although point photodetectors, notably photomultiplier tubes (PMTs), have been commonly used in laser scanning microscopy (LSM) with a confocal illumination setup, wide-field photodetectors, such as electron-multiplying charge-coupled devices (EMCCDs) and scientific complementary metal-oxide-semiconductor (sCMOS) cameras have been used in fluorescence imaging. This review focuses on the former low-light point photodetectors and presents their fluorescence microscopy applications and recent progress. These photodetectors include conventional PMTs, single photon avalanche diodes (SPADs), hybrid photodetectors (HPDs), in addition to newly emerging photodetectors, such as silicon photomultipliers (SiPMs) (also known as multi-pixel photon counters (MPPCs)) and superconducting nanowire single photon detectors (SSPDs). In particular, this review shows distinctive features of HPD and application of HPD to wide-field single-molecule fluorescence detection.
Influence of Silver Nanoparticles, Laser Light and Electromagnetic Stimulation of Seeds on Germination Rate and Photosynthetic Parameters in Pumpkin (Cucurbita pepo L.) Leaves
Authors: Dziwulska-Hunek Agata , Kachel Magdalena , Gagoś Mariusz , Szymanek Mariusz
Published: March 2021

The study aimed to determine the impact of laser light (L), magnetic stimulation (p) and silver nanocolloid (AgNC) on pumpkin seeds on the germination rate and content of photosynthetic pigments as well as the efficiency of photosynthesis and greenness index in the plant’s development stages. Seeds germinated after the use of various combinations of different refining techniques. The best results were observed for the alternating magnetic field, where the germination energy increased significantly by 20% relative to the control. A similar effect was observed in terms of germination capacity which increased by 4%. A decrease in terms of emergence rate was observed in all study groups. Leaves grown from seeds soaked in nanocolloidal silver on platters were characterised by a significantly higher content of chlorophyll a and b by, respectively, 53 and 11%, as well as 79% higher carotenoid content. The leaves of potted plants contained 42 and 43% more chlorophyll a in groups p and AgNC. In addition, 66 and 81% more carotenoids in groups L and p. At the stage of the onset of flowering of pumpkin, an improvement in terms of photosynthetic efficiency and greenness index was observed in all study groups. The highest improvement was recorded for seeds soaked in silver and reached 23% (intensity of photosynthesis) and 11% (greenness index SPAD).
Tropospheric NO2 Pollution Monitoring with the GF-5 Satellite Environmental Trace Gases Monitoring Instrument over the North China Plain during Winter 2018–2019
Authors: Yang Dongshang , Luo Yuhan , Zeng Yi , Si Fuqi , Xi Liang , Zhou Haijin , Liu Wenqing
Published: March 2021

The Environmental Trace Gases Monitoring Instrument (EMI) is a high-spectral-resolution payload onboard the latest pathfinder mission GaoFen-5, designed specifically for the monitoring of global atmospheric trace gas compositions and trends. This study describes a comparative analysis of the tropospheric nitrogen dioxide (NO2) columns over the North China Plain (NCP) from November 2018 to April 2019 based on EMI products. Validation of satellite products based on a cross-correlation analysis with data from four ground-based multi-axis differential optical absorption spectroscopy sites provided good correlation coefficients (r) ranging from 0.78 to 0.88. The distribution and monthly averaged tropospheric NO2 columns revealed high pollution exposure levels during winter (November–January) and a decrease from February onward in the NCP. Moreover, a typical pollution event was analyzed in detail in combination with wind field statistics. The results indicated that variations of NO2 concentrations in Beijing and Tianjin were highly correlated with the wind direction from 22.5–45.0 degrees west of south, especially during times of high NO2 amounts. These findings highlight that the EMI payload on the GaoFen-5 (GF-5) satellite is useful for remote sensing of regional and global NO2 detection.
Improved Algorithms for Remote Sensing-Based Aerosol Retrieval during Extreme Biomass Burning Events
Authors: Mukai Sonoyo , Sano Itaru , Nakata Makiko
Published: March 2021

This study proposed an aerosol characterization process using satellites for severe biomass burning events. In general, these severely hazy cases are labeled as “undecided” or “hazy.” Because atmospheric aerosols are significantly affected by factors such as air quality, global climate change, local environmental risk, and human and biological health, efficient and accurate algorithms for aerosol retrieval are required for global satellite data processing. Our previous classification of aerosol types was based primarily on near-ultraviolet (UV) data, which facilitated subsequent aerosol retrieval. In this study, algorithms for aerosol classification were expanded to events with serious biomass burning aerosols (SBBAs). Once a biomass burning event is identified, the appropriate radiation simulation method can be applied to characterize the SBBAs. The second-generation global imager (SGLI) on board the Japanese mission JAXA/Global Change Observation Mission-Climate contains 19 channels, including red (674 nm) and near-infrared (869 nm) polarization channels with a high resolution of 1 km. Using the large-scale wildfires in Kalimantan, Indonesia in 2019 as an example, the complementarity between the polarization information and the nonpolarized radiance measurements from the SGLI was demonstrated to be effective in radiation simulations for biomass burning aerosol retrieval. The retrieved results were verified using NASA/AERONET ground-based measurements, and then compared against JAXA/SGLI/L2-version-1 products, and JMA/Himawari-8/AHI observations.
Species of Fungi and Pollen in the PM1 and the Inhalable Fraction of Indoor Air in Homes
Authors: Lu Rui , Pørneki Ann Dorte , Lindgreen Jonas N. , Li Yanpeng , Madsen Anne Mette
Published: March 2021

Airborne microbial fragments in the PM1 fraction (particles with aerodynamic diameters less than 1 µm) are a cause for concern as they may potentially deposit in the alveoli of the human airways. This study aimed to use qPCR to identify and quantify 24 different species or groups of genera in the PM1 and the inhalation fraction (particles that may enter the mouth or nose during breathing) of indoor air and to relate this to what has previously been found for each species. Results showed that eight fungal species, and Aspergillus/Penicillium/Paecilomyces variotii, as well as Alnus/Corylus and actinobacteria belonging to the Streptomyces genus were detected both in the PM1 and the inhalable fraction. Five fungal species were only detected in the inhalable fraction. A significant effect of season was found on the fungal composition in the PM1 (p = 0.001) and the inhalable (p = 0.017) fraction. This study demonstrated that it is possible to use qPCR to identify and quantify different microbes in the PM1 fraction, and it has improved our understanding of the qualitative and quantitative relationship between the PM1 and the inhalable microbial particles in indoor air. Combined with the literature review it also shows a large variation within and between species in the share of fungi which is present as fragments.
Energy-Efficiency Requirements for Residential Building Envelopes in Cold-Climate Regions
Authors: Huynh Amy , Dias Barkokebas Regina , Al-Hussein Mohamed , Cruz-Noguez Carlos , Chen Yuxiang
Published: March 2021

Due to the energy and environmental impacts attributed to the operational phase of the building sector, efforts have been made to improve building energy performance through the implementation of restrictive energy requirements by regulatory bodies. In this context, the primary objective of this paper is to investigate and compare regulations that govern the building envelope energy performance of new residential buildings in cold-climate regions, primarily in Canada, Finland, Iceland, Norway, Sweden, China, and Russia. The aim is to identify similarities and dissimilarities among the energy regulations of these countries, as well as potentials for development of more effective building codes. This study verifies that the investigated energy requirements diverge considerably—for instance, the required thermal resistance per unit area of above-grade exterior walls in Sweden is almost two times that of a similar climate zone in Canada. Based on the comparisons and case analyses, recommendations for energy requirements pertinent to building envelope of new residential buildings in cold-climate regions are proposed.