bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigation into the Classification of Tight Sandstone Reservoirs via Imbibition Characteristics
Authors: Li Ming , Yang Hai’en , Lu Hongjun , Wu Tianjiang , Zhou Desheng , Liu Yafei
Published: October 2018

Tight sandstone reservoirs are often produced by shutting in the well and inducing imbibition. However, by adopting current reservoir classifications, the heterogeneity of reservoirs cannot be properly treated. Based upon the analysis of the imbibition curves and mercury intrusion porosimetry tests, Chang-7 tight sandstone reservoirs were classified into three categories according to the newly proposed standards. Imbibition tests demonstrated that for the first category, imbibition and drainage occurred continuously and never reached the plateau within the experiment duration. It was suggested that a longer shut-in time favors the production of oil. For the second category, a steady state for imbibition was reached and a shut-in time as short as three days resulted in a high imbibition rate. For the third category, a plateau was reached for the first time and imbibition restarted until a steady state was reached. The average shut-in time for the third category was eight days. Compatibility between reservoir characteristics and a soaking development regime based upon the proposed classification methods effectively enhances the oil recovery efficiency of formations with distinct petrophysical properties. This provides insight into the classification methods of tight sandstone reservoirs.
Short-Term Vegetation Responses to Invasive Shrub Control Techniques for Amur Honeysuckle (Lonicera maackii [Rupr.] Herder)
Authors: Frank Graham S. , Saunders Michael R. , Jenkins Michael A.
Published: October 2018

Invasive shrubs in forest understories threaten biodiversity and forest regeneration in the eastern United States. Controlling these extensive monotypic shrub thickets is a protracted process that slows the restoration of degraded forest land. Invasive shrub removal can be accelerated by using forestry mulching heads, but evidence from the western United States indicates that mulching heads can promote exotic species establishment and mulch deposition can reduce native plant species abundance. We compared the effectiveness of the mulching head and the “cut-stump” method for controlling the invasive shrub Amur honeysuckle (Lonicera maackii), as well as their impacts on native plant community recovery, in mixed-hardwood forests of Indiana. After two growing seasons, mulching head treatment resulted in greater L. maackii regrowth and regeneration. The recovery of native plant abundance and diversity following shrub removal did not differ between the two methods. However, mulch deposition was associated with increased abundance of garlic mustard (Alliaria petiolata), an invasive forb. Increasing mulching head treatment depth reduced L. maackii regrowth, but additional study is needed to determine how it affects plant community responses. The mulching head is a promising technique for invasive shrub control and investigating tradeoffs between reducing landscape-scale propagule pressure and increased local establishment will further inform its utility.
Decreased Capacity for Sperm Production Induced by Perinatal Bisphenol A Exposure Is Associated with an Increased Inflammatory Response in the Offspring of C57BL/6 Male Mice
Authors: Meng Yuan , Lin Ren , Wu Fengjuan , Sun Qi , Jia Lihong
Published: October 2018

Many previous studies have indicated the adverse effects of bisphenol A (BPA) on sperm production and quality; however, the mechanisms underlying BPA male reproductive toxicity have yet to be elucidated. The main purpose of this study was to investigate the effect of perinatal exposure to BPA on the spermatogenic capacity of male offspring, and to explore the possible influence of inflammatory responses in BPA reproductive toxicity. Twenty-one pregnant C57BL/6mice were randomly divided into three groups: a control group, a group receiving 0.2 μg/mL (LBPA), and a group receiving 2 μg/mL of BPA (HBPA), all via drinking water from gestational day 6 to the end of lactation. After weaning, one male mouse was randomly selected from each group (n = 7/group); these three mice were fed a normal diet and drinking water for 1 month. Levels of serum testosterone (T) and tumor necrosis factor (TNF)-α were then measured in all mice. Sperm count and the proportion of sperm malformation were also determined. The levels of Toll-like receptor 4 (TLR4), nuclear factor (NF)-κB, and aryl hydrocarbon receptor (AhR) protein expression in the testis tissue were determined. Analysis showed that the proportion of sperm malformation increased in the LBPA and HBPA groups (p < 0.05). Sperm count significantly decreased only in the HBPA group (p < 0.05), while the levels of serum TNF-α increased in the LBPA and HBPA groups (p < 0.05). Levels of serum T decreased significantly in the HBPA group, compared with controls (p < 0.05). Levels of TLR4 and NF-κB protein expression in the testis were significantly higher in the LBPA and HBPA groups (p < 0.05 or p < 0.01), while AhR protein expression was higher and seminiferous tubules in the testis showed more damage in the HBPA group compared to controls (p < 0.05 and p < 0.01, respectively). Our results showed that perinatal exposure to low or high doses of BPA decreased the capacity for spermatogenesis in male offspring, which may be associated with an inflammatory response activated by the TLR4/ NF-κB and AhR signaling pathways in the testis.
TIPE Family of Proteins and Its Implications in Different Chronic Diseases
Authors: Bordoloi Devivasha , Banik Kishore , Shabnam Bano , Padmavathi Ganesan , Monisha Javadi , Arfuso Frank , Dharmarajan Arunasalam , Mao Xinliang , Lim Lina H. K. , Wang Lingzhi , Fan Lu , Hui Kam Man , Kumar Alan Prem , Sethi Gautam , Kunnumakkara Ajaikumar B.
Published: October 2018

The tumor necrosis factor-α-induced protein 8-like (TIPE/TNFAIP8) family is a recently identified family of proteins that is strongly associated with the regulation of immunity and tumorigenesis. This family is comprised of four members, namely, tumor necrosis factor-α-induced protein 8 (TIPE/TNFAIP8), tumor necrosis factor-α-induced protein 8-like 1 (TIPE1/TNFAIP8L1), tumor necrosis factor-α-induced protein 8-like 2 (TIPE2/TNFAIP8L2), and tumor necrosis factor-α-induced protein 8-like 3 (TIPE3/TNFAIP8L3). Although the proteins of this family were initially described as regulators of tumorigenesis, inflammation, and cell death, they are also found to be involved in the regulation of autophagy and the transfer of lipid secondary messengers, besides contributing to immune function and homeostasis. Interestingly, despite the existence of a significant sequence homology among the four members of this family, they are involved in different biological activities and also exhibit remarkable variability of expression. Furthermore, this family of proteins is highly deregulated in different human cancers and various chronic diseases. This review summarizes the vivid role of the TIPE family of proteins and its association with various signaling cascades in diverse chronic diseases.
METCAM/MUC18 Decreases the Malignant Propensity of Human Ovarian Carcinoma Cells
Authors: Wu Guang-Jer
Published: October 2018

METCAM/MUC18 is an integral membrane cell adhesion molecule (CAM) in the Ig-like gene super-family. It can carry out common functions of CAMs which is to perform intercellular interactions and interaction of cell with extracellular matrix in tumor microenvironment, to interact with various signaling pathways and to regulate general behaviors of cells. We and other two groups previously suggested that METCAM/MUC18 probably be utilized as a biomarker for predicting the malignant tendency of clinical ovarian carcinomas, since METAM/MUC18 expression appears to associate with the carcinoma at advanced stages. It has been further postulated to promote the malignant tendency of the carcinoma. However, our recent research results appear to support the conclusion that the above positive correlation is fortuitous; actually METCAM/MUC18 acts as a tumor and metastasis suppressor for the ovarian carcinoma cells. We also suggest possible mechanisms in the METCAM/MUC18-mediated early tumor development and metastasis of ovarian carcinoma. Moreover, we propose to employ recombinant METCAM/MUC18 proteins and other derived products as therapeutic agents to treat the ovarian cancer patients by decreasing the malignant potential of ovarian carcinoma.
Anthocyanins Extracted from Oryza sativa L. Prevent Fluorouracil-Induced Nuclear Factor-κB Activation in Oral Mucositis: In Vitro and In Vivo Studies
Authors: Tancharoen Salunya , Shakya Prana , Narkpinit Somphong , Dararat Pornpen , Kikuchi Kiyoshi
Published: October 2018

This study aims to investigate the immunomodulatory effect of anthocyanins (ANTs) from Oryza sativa L. extracts on 5-fluorouracil (5-FU)-induced oral mucositis, using a rat model and oral keratinocytes. ANTs were detected by high-performance liquid chromatography (HPLC)-electrospray ionization mass spectrometry. Animals were randomly given varying doses of ANT-rich extract treatment (500 mg/kg and 1000 mg/kg) in the absence or presence of 5-FU-induced mucositis. Buccal mucosae were photographed and scored for macroscopic analysis and incisional biopsies of cheek pouches were collected for microscopic examination of oral mucositis. 5-FU caused marked hemorrhage, extensive ulcerations and abscesses compared to non-treated animals with slight erythema. Histologically, a loss of collagen bundles and inflammatory cell infiltrates was observed. After 29 days of ANT treatment, lesions resolved, and abundant collagen fibers were evident in the lamina propria. Buccal mucosa of 5-FU-injected rats showed increased Nuclear factor-kappa B (NF-κB) p50 and p65 in oral keratinocytes. The administration of ANT reduced NF-κB-positive cells in 5-FU rats (p < 0.001) compared to the non-treatment group. In oral keratinocytes, ANT treatment significantly restored 5-FU-induced growth inhibition and impaired the nuclear accumulation of NF-κB p50 and p65. Our study demonstrated that ANT from Oryza sativa L. exhibited effective anti-inflammatory properties against 5-FU-induced oral mucositis by inhibiting NF-κB activation.
Directed Evolution of a Homodimeric Laccase from Cerrena unicolor BBP6 by Random Mutagenesis and In Vivo Assembly
Authors: Zhang Ji , Ma Fuying , Zhang Xiaoyu , Geng Anli
Published: October 2018

Laccases have great potential for industrial applications due to their green catalytic properties and broad substrate specificities, and various studies have attempted to improve the catalytic performance of these enzymes. Here, to the best of our knowledge, we firstly report the directed evolution of a homodimeric laccase from Cerrena unicolor BBP6 fused with α-factor prepro-leader that was engineered through random mutagenesis followed by in vivo assembly in Saccharomyces cerevisiae. Three evolved fusion variants selected from ~3500 clones presented 31- to 37-fold increases in total laccase activity, with better thermostability and broader pH profiles. The evolved α-factor prepro-leader enhanced laccase expression levels by up to 2.4-fold. Protein model analysis of these variants reveals that the beneficial mutations have influences on protein pKa shift, subunit interaction, substrate entrance, and C-terminal function.
OPNa Overexpression Is Associated with Matrix Calcification in Thyroid Cancer Cell Lines
Authors: Ferreira Luciana B. , Lima Raquel T. , Bastos Ana Clara Santos da Fonseca , Silva Andreia M. , Tavares Catarina , Pestana Ana , Rios Elisabete , Eloy Catarina , Sobrinho-Simões Manuel , Gimba Etel R. P. , Soares Paula
Published: October 2018

Osteopontin (OPN) spliced variants (OPN-SV: OPNa, OPNb, and OPNc) are aberrantly expressed in tumors and frequently associated with cancer progression. This holds true for papillary thyroid carcinoma (PTC), which is the most common type of thyroid cancer (TC). PTC often presents with desmoplasia and dystrophic calcification, including psammoma bodies (PB). This work aimed to investigate total OPN (tOPN) and OPN-SV expression and their association with the presence of PB in the PTC classical variants (cPTC), as well as the involvement of OPN-SV in matrix calcification of TC cell lines. We found that cPTC samples presenting PB showed higher OPN expression levels. In TC cell lines, OPNa overexpression promotes higher matrix calcification and collagen synthesis when compared to that of clones overexpressing OPNb or OPNc. In response to OPN knockdown, calcification was inhibited, paralleled with the downregulation of calcification markers. In conclusion, our data evidenced that OPN expression is associated with the presence of PB in cPTC samples. Among the OPN-SV, OPNa is the main contributor to matrix calcification in tested TC cells, providing clues to a better understanding on the biology and ethiopathogenesis of the calcification process in TC cells.
The Role of Physical Stimuli on Calcium Channels in Chondrogenic Differentiation of Mesenchymal Stem Cells
Authors: Uzieliene Ilona , Bernotas Paulius , Mobasheri Ali , Bernotiene Eiva
Published: October 2018

Human mesenchymal stem cells (hMSC) are becoming increasingly popular in tissue engineering. They are the most frequently used stem cell source for clinical applications due to their high potential to differentiate into several lineages. Cartilage is known for its low capacity for self-maintenance and currently there are no efficient methods to improve cartilage repair. Chondrogenic differentiation of hMSC isolated from different tissues is widely employed due to a high clinical demand for the improvement of cartilage regeneration. Calcium channels that are regulated by physical stimuli seem to play a pivotal role in chondrogenic differentiation of MSCs. These channels increase intracellular calcium concentration, which leads to the initiation of the relevant cellular processes that are required for differentiation. This review will focus on the impact of different physical stimuli, including electrical, electromagnetic/magnetic and mechanical on various calcium channels and calcium signaling mechanisms during chondrogenic differentiation of hMSC.
Ghrelin in Serum and Urine of Post-Partum Women with Gestational Diabetes Mellitus
Authors: Kimber-Trojnar Żaneta , Patro-Małysza Jolanta , Skórzyńska-Dziduszko Katarzyna E. , Oleszczuk Jan , Trojnar Marcin , Mierzyński Radzisław , Leszczyńska-Gorzelak Bożena
Published: October 2018

Women with a previous history of gestational diabetes mellitus (GDM) have a significantly increased risk of developing type 2 diabetes, obesity, and cardiovascular diseases in the future. The aim of the study was to evaluate ghrelin concentrations in serum and urine in the GDM group in the early post-partum period, with reference to laboratory results, body composition, and hydration status. The study subjects were divided into two groups, that is, 28 healthy controls and 26 patients with diagnosed GDM. The maternal body composition and hydration status were evaluated by the bioelectrical impedance analysis (BIA) method. The concentrations of ghrelin in the maternal serum and urine were determined via enzyme-linked immunosorbent assay (ELISA). The laboratory and BIA results of the mothers with GDM were different from those without GDM. Urine ghrelin positively correlated with serum ghrelin and high-density lipoprotein cholesterol (HDL) levels in healthy mothers. There were direct correlations between urine ghrelin and HDL as well as triglycerides levels in the GDM group. Neither the lean tissue index nor body cell mass index were related to the serum ghrelin concentrations in this group. Only the urine ghrelin of healthy mothers correlated with the fat tissue index. Our results draw attention to urine as an easily available and appropriable biological material for further studies.
aP2-Cre Mediated Ablation of GHS-R Attenuates Adiposity and Improves Insulin Sensitivity during Aging
Authors: Lin Ligen , Lee Jong , Wang Ruitao , Wang Ru , Sheikh‐Hamad David , Zang Qun , Sun Yuxiang
Published: October 2018

Ghrelin via its receptor, the growth hormone secretagogue receptor (GHS-R), increases food intake and adiposity. The tissue-specific functions of GHS-R in peripheral tissues are mostly unknown. We previously reported that while GHS-R expression is very low in white and brown fat of young mice, expression increases during aging. To investigate whether GHS-R has cell-autonomous effects in adipose tissues, we generated aP2-Cre-mediated GHS-R knockdown mice (aP2-Cre/Ghsrf/f). We studied young (5–6 months) and old (15–17 months) aP2-Cre/Ghsrf/f mice and their age-matched controls. Interestingly, young aP2-Cre/Ghsrf/f mice had normal body weight but reduced fat; old mice showed pronounced reductions of both body weight and body fat. Calorimetry analysis revealed that aP2-Cre/Ghsrf/f mice had normal food intake and locomotor activity at both young and old age; but intriguingly, while energy expenditure was normal at young age, it was significantly increased at old age. Both young and old aP2-Cre/Ghsrf/f mice exhibited improved insulin sensitivity and glucose tolerance. Importantly, old aP2-Cre/Ghsrf/f mice maintained higher core body temperature at 4 °C, and showed higher expression of the thermogenic uncoupling protein 1 (UCP1) gene. The ex vivo studies further demonstrated that GHS-R deficient white adipocytes from old mice exhibit increased glucose uptake and lipolysis, promoting lipid mobilization. Despite the fact that the in vivo phenotypes of aP2-Cre/Ghsrf/f mice may not be exclusively determined by GHS-R knockdown in adipose tissues, our data support that GHS-R has cell-autonomous effects in adipocytes. The anabolic effect of GHS-R in adipocytes is more pronounced in aging, which likely contributes to age-associated obesity and insulin resistance.
Performance Analysis of Honeypot with Petri Nets
Authors: Shi Leyi , Li Yang , Feng Haijie
Published: October 2018

As one of the active defense technologies, the honeypot deceives the latent intruders to interact with the imitated systems or networks deployed with security mechanisms. Its modeling and performance analysis have not been well studied. In this paper, we propose a honeypot performance evaluation scheme based on Stochastic Petri Nets (SPN). We firstly set up performance evaluation models for three types of defense scenarios (i.e., firewall; firewall and Intrusion Detection System (IDS); firewall, IDS and honeypot) based on SPN. We then theoretically analyze the SPN models by constructing Markov Chains (MC), which are isomorphic to the models. With the steady state probabilities based on the MC, the system performance evaluation is done with theoretical inference. Finally, we implement the proposed three SPN models on the PIPE platform. Five parameters are applied to compare and evaluate the performance of the proposed SPN models. The analysis of the probability and delay of three scenarios shows that the simulation results validate the effectiveness in security enhancement of the honeypot under the SPN models.
Validity of Capsule Endoscopy in Monitoring Therapeutic Interventions in Patients with Crohn’s Disease
Authors: Nakamura Masanao , Yamamura Takeshi , Maeda Keiko , Sawada Tsunaki , Mizutani Yasuyuki , Ishikawa Takuya , Furukawa Kazuhiro , Ohno Eizaburo , Kawashima Hiroki , Miyahara Ryoji , Koulaouzidis Anastasios , Hirooka Yoshiki ,   The Nagoya University Crohn’s Disease Study Group
Published: October 2018

Mucosal healing in Crohn’s disease (CD) can be evaluated by capsule endoscopy (CE). However, only a few studies have utilized CE to demonstrate the therapeutic effect of medical treatment. We sought to evaluate the validity of using CE to monitor the effect of medical treatment in patients with CD. One hundred (n = 100) patients with CD were enrolled. All patients had a gastrointestinal (GI) tract patency check prior to CE. Patients with baseline CE Lewis score (LS) ≤ 135 were included in the non-active CD group and ended the study. In those with LS > 135 (active CD group), additional treatment was administered, regardless of symptoms, as per the treating clinician’s advice. Patients of the active CD group underwent follow-up CE assessment 6 months later. Out of 92 patients with confirmed GI patency who underwent CE, 40 (43.4%) had CE findings of active inflammation. Of 29 patients with LS > 135 who received additional medications and underwent follow-up CE, improvement of the LS was noted in 23 (79.3%) patients. Eleven patients were asymptomatic but received additional medications; 8 (72.7%) had improvement of the LS. This study demonstrated that additional treatment even for patients with CD in clinical remission and active small-bowel inflammation on CE can reduce mucosal damage.
Impact of Age-Adjusted Charlson Comorbidity on Hospital Survival and Short-Term Outcome of Patients with Extracorporeal Cardiopulmonary Resuscitation
Authors: Tseng Li-Jung , Yu Hsi-Yu , Wang Chih-Hsien , Chi Nai-Hsin , Huang Shu-Chien , Chou Heng-Wen , Shih Hsin-Chin , Chou Nai-Kuan , Chen Yih-Sharng
Published: October 2018

Extracorporeal cardiopulmonary resuscitation (ECPR) has gradually come to be regarded as an effective therapy, but the hospital mortality rate after ECPR is still high and unpredictable. The present study tested whether age-adjusted Charlson comorbidity index (ACCI) can be used as an objective selection criterion to ensure the most efficient utilization of medical resources. Adult patients (age ≥ 18 years) receiving ECPR at our institution between 2006 and 2015 were included. Data regarding ECPR events and ACCI characteristics were collected immediately after the extracorporeal membrane oxygenation (ECMO) setup. Adverse events during hospitalization were also prospectively collected. The primary endpoint was survival to hospital discharge. The second endpoint was the short-term (2-year) follow-up outcome. A total of 461 patients included in the study were grouped into low ACCI (ACCI 0–3) (240, 52.1%) and high ACCI (ACCI 4–13) (221, 47.9%) groups. The median ACCI was 2 (interquartile range (IQR): 1–3) and 5 (IQR: 4–7) for the low and high ACCI groups, respectively. Cardiopulmonary resuscitation (CPR)-to-ECMO duration was comparable between the groups (42.1 ± 25.6 and 41.3 ± 20.7 min in the low and high ACCI groups, respectively; p = 0.754). Regarding the hospital survival rate, 256 patients (55.5%) died on ECMO support. A total of 205 patients (44.5%) were successfully weaned off ECMO, but only 138 patients (29.9%) survived to hospital discharge (32.1% and 27.6% in low and high ACCI group, p = 0.291). Multivariate logistic regression analysis revealed CPR duration before ECMO run (CPR-to-ECMO duration) and a CPR cause of septic shock to be significant risk factors for hospital survival after ECPR (p = 0.043 and 0.014, respectively), whereas age and ACCI were not (p = 0.334 and 0.164, respectively). The 2-year survival rate after hospital discharge for the 138 hospital survivors was 96% and 74% in the low and high ACCI groups, respectively (p = 0.002). High ACCI before ECPR does not predict a poor outcome of hospital survival. Therefore, ECPR should not be rejected solely due to high ACCI. However, high ACCI in hospital survivors is associated with a higher 2-year mortality rate than low ACCI, and patients with high ACCI should be closely followed up.
Europium, Yttrium, and Indium Recovery from Electronic Wastes
Authors: De la Torre Ernesto , Vargas Estefanía , Ron César , Gámez Sebastián
Published: October 2018

Waste electrical and electronic equipment (WEEE) has increased in recent decades due to the continuous advancement of technology in the modern world. These residues have various metals that are found in concentrations that make their recovery profitable. A group of metals of interest are the rare earths such as europium and yttrium, as well as semiconductors such as indium. Yttrium was recovered from cathode ray tubes that were manually dismantled. The resulted powder was leached with HNO3, and then the solution was submitted to solvent extraction with di-(2-ethylhexyl) phosphoric acid (DEHPA) using n-heptane as a diluent. For re-extraction, HNO3 was used again, and yttrium was precipitated by adding four times the stoichiometric amount of oxalic acid, reaching 68% yttrium purity. Indium was recovered from the liquid crystal display (LCD) screens for which the pulverized material was leached with H2SO4. Then, the indium sulfate was subjected to solvent extraction using DEHPA as an extractant, and diesel as a diluent. The re-extraction was carried out again with H2SO4, and the obtained acid solution was evaporated until the indium precipitated, reaching a recovery of 95%. The investigations that were carried out show that it is feasible to recover these metals in the form of oxides or phosphates with high commercial value.
The Effect of Microstructural Characteristics on the Hydrogen Permeation Transient in Quenched and Tempered Martensitic Alloys
Authors: Van den Eeckhout E. , Depover T. , Verbeken K.
Published: October 2018

This work evaluates the permeation curve characteristics for four quenched and tempered generic, ternary alloys, each containing one specific carbide. The different carbides (W2C, Cr23C6, TiC, and V4C3, respectively) are induced by a quench and tempering treatment. The correlation is made between the different microstructural characteristics, including the carbides and the martensitic matrix, and the observed hydrogen diffusivity and thus the permeation transient. The permeation curves, obtained via the Devanathan and Stachurski method, are therefore compared with thermal desorption spectroscopy and hot extraction results. The delay of the permeation transient can be associated with the overall trap density, while the slope is related to the amount of reversible trapping sites. Generally, the obtained hydrogen permeation transient of the different ternary or Fe–C–X materials correlates with the hydrogen trapping ability. The following order of hydrogen diffusion is determined, i.e., Fe–C–V < Fe–C–Ti << Fe–C–Cr < Fe–C–W. The hydrogen trapping ability of the tempered induced carbides plays a decisive role in the value of the hydrogen diffusion coefficient.
Investigation of Copper-Aluminium Composite Materials Produced by Explosive Welding
Authors: Kaya Yakup
Published: October 2018

Aluminium and copper are two metals frequently used in the automotive and aerospace industries due to their properties of lightness and high conductivity. In this study, copper and aluminium plates were joined using the explosive welding method. The effects of the explosive ratio on the properties of the bonding interface were investigated. Results of the experimental studies showed that the bonding interface changed from a slightly wavy structure to a completely wavy structure as the explosive ratio was increased. It was found that due to the cold deformation resulting from the collision of the flyer and parent plates during the explosion, there was an increase in the hardness values near the bonding interface and on the outer surfaces of the plates. The increase in deformation hardening along with the increasing explosive ratios led to the reduced impact toughness of the composites. In the results of the tensile-shear and bending tests, no separation or fracturing was seen in the bonding interfaces at any of the explosive ratios. As a result, it was seen that the explosive welding method can be used in combining copper and aluminium materials.
Study of the Mechanical Behavior of Dual-Phase Steel Based on Crystal Plasticity Modeling Considering Strain Partitioning
Authors: Xu Yongsheng , Dan Wenjiao , Ren Chuang , Huang Tingting , Zhang Weigang
Published: October 2018

The similar crystal structures of martensite (BCT) and ferrite (BCC) cause difficulty in distinguishing the grain orientations of individual phases in dual-phase (DP) steels. A dislocation-based multiphase mixed hardening model is presented, considering both ferrite and martensite strain partitioning, to describe the texture-dependent mechanical behavior of DP steels more precisely. This model is based on the ideals that (i) the volume fractions of the constituent phases and the corresponding strain partitioning function are obtained through in situ tensile experimentation, and (ii) the grain orientations of ferrite and martensite are assumed to be in accordance with the overall texture. We applied the model to calculate the macroscopic and microscopic mechanical behavior of DP800 steel using a crystal plasticity finite element (CPFE) code. The results show that the calculated stress-strain response and textural evolution are in good agreement with the experimental results. The dislocation evolution indicates that the rapid hardening of ferrite induces a high hardening rate for DP steels early in plastic deformation. In addition, for the grains corresponding to the texture center orientations of DP800, the activity and dislocation density evolutions of the slip systems are studied.
Tailoring of Microstructures and Tensile Properties in the Solidification of Al-11Si(-xCu) Brazing Alloys
Authors: Donadoni Bruno Monti Carmelo , Gomes Leonardo Fernandes , Garcia Amauri , Spinelli José Eduardo
Published: October 2018

Ternary Al-11wt %Si-(xwt %)Cu alloys are highly recommended as commercial filler metals for aluminum brazing alloys. However, very little is known about the functional inter-relations controlling the solidified microstructures characterizing processes such as torch and furnace brazing. As such, we evaluated two commercial brazing alloys, which are the Al-11wt %Si-3.0wt %Cu and Al-11wt %Si-4.5wt %Cu alloys: Cu contents typically trend in between the suitable alloying spectrum. We analyzed the effects of solidification kinetics over features such as the dendrite arm spacing and the spacing between particles constituting the eutectic mixture. Also, tensile properties were determined as a function of the dendrite microstructure dimensions. The parameters concerned for translating the solidification kinetics were either the cooling rate, or growth velocity related to the displacement of the dendrite tip, or the eutectic front. The relevant scaling laws representing the growth of these brazing alloys are outlined. The experimental results demonstrated that a 50% increase in Cu alloying (from 3.0 to 4.5 wt %) could be operated in order to obtain significant variations in the dendritic length-scale of the microstructure across the produced parts. Overall, the microstructures were constituted by an α-Al dendritic matrix surrounded by a ternary eutectic consisting of α-Al + Al2Cu + Si. The scale measurements committed to the Al2Cu eutectic phase pointed out that the increase in Cu alloying has a critical role on refining the ternary eutectic.
Vibration-Assisted Roll-Type Polishing System Based on Compliant Micro-Motion Stage
Authors: Gu Yan , Chen Xiuyuan , Lin Jieqiong , Lu Mingming , Lu Faxiang , Zhang Zheming , Yang Hao
Published: October 2018

This paper aims to create a high-quality surface based on the linear contact material removal mechanism. For this paper, a piezo-driven, flexure-based micro-motion stage was developed for the vibration-assisted roll-type precision polishing system. Meanwhile, the compliance matrix method was employed to establish the amplification ratio and compliance model of the flexure mechanism. The dimensions of the mechanism were optimized using the grey wolves optimization (GWO) algorithm, aiming to maximize the natural frequencies. Using the optimal parameters, the established models for the mechanical performance evaluation of the flexure stage were verified with the finite-element method. Through closed-loop test, it was proven that the proposed micro-motion stage performs well in positioning micro motions. Finally, high quality surface using silicon carbide (SiC) ceramic with 36 nm Sa was generated by the independently developed vibration-assisted roll-type polishing machine to validate the performance of the established polishing system.
Petrogenesis and Metallogenic Implications of Neoproterozoic Granodiorite in the Super-Large Shimensi Tungsten-Copper Deposit in Northern Jiangxi, South China
Authors: Wei Wenfeng , Lai Chunkit , Yan Bing , Zhu Xiaoxi , Song Shengqiong , Liu Lei
Published: October 2018

The newly discovered Shimensi deposit is a super-large tungsten-copper (W–Cu) deposit with a metal reserve of 742.55 thousand tonnes (kt) W and 403.6 kt Cu. The orebodies are hosted in Mesozoic granites, which intruded the poorly documented Shimensi granodiorite belonging to the Jiuling batholith, the largest intrusion (outcrop > 2500 km2) in South China. Our new SHRIMP (Sensitive High Resolution Ion MicroProbe) zircon dating revealed that the granodiorite at Shimensi (ca. 830–827 Ma) was formed coeval (within analytical uncertainty) or slightly earlier than those in many other places (ca. 819–807 Ma) of the Jiuling batholith. The Neoproterozoic Shimensi granodiorite is peraluminous and high-K calc-alkaline, and contains low P content with no S-type trend (positive P2O5 vs. SiO2 correlation) displayed, thus best classified as peraluminous I-type. The I-type classification is also supported by the zircon REE patterns, largely (93%) positive εHf(t) (−0.87 to 6.60) and relatively low δ18O (5.8–7.7‰). The Neoproterozoic Shimensi granodiorite was formed after the continental arc magmatism (ca. 845–835 Ma), but before the post-collisional S-type granite emplacement (ca. 825–815 Ma) in the Jiangnan Orogen. Therefore, we propose that the Shimensi granodiorite was formed in a collisional/early post-collisional setting. The δ18O increase from the Shimensi granodiorite to many younger (ca. 819–807 Ma) granodiorites (6.0–8.5‰) in the Jiuling batholith probably reflects an increase of supracrustal rock-derived melts with the progress of collision. The Shimensi granodiorite contains low zircon Ce4+/Ce3+ and Eu/Eu*, suggesting a relatively reducing magma that does not favor porphyry Cu–Au mineralization. This left a high background Cu concentration (avg. 196 ppm) in the Neoproterozoic granodiorite, which may have contributed to the Mesozoic W–Cu mineralization, when the granodiorite is intruded and assimilated by the Mesozoic granites.
A Label-Free Fluorescent DNA Calculator Based on Gold Nanoparticles for Sensitive Detection of ATP
Authors: Zhang Jingjing , Zhang Shizhi , Niu Chaoqun , Liu Chen , Du Jie , Chen Yong
Published: October 2018

Herein we described a deoxyribonucleic acid (DNA) calculator for sensitive detection of the determination of adenosine triphosphate (ATP) using gold nanoparticles (GNP) and PicoGreen fluorescence dye as signal transducer, and ATP and single-stranded DNA (DNA-M′) as activators. The calculator-related performances including linearity, reaction time, logic gate, and selectivity were investigated, respectively. The results revealed that this oligonucleotide sensor was highly sensitive and selective. The detection range was 50–500 nmol/L (R2 = 0.99391) and the detection limit was 46.5 nmol/L. The AND DNA calculator was successfully used for the ATP detection in human urine. Compared with other methods, this DNA calculator has the characteristics of being label-free, non-enzymic, simple, and highly sensitive.
Correction: de Gonzalo, G. Lipase Catalysed Kinetic Resolution of Racemic 1,2-Diols Containing a Chiral Quaternary Center. Molecules 2018, 23, 1585
Authors: De Gonzalo Gonzalo
Published: October 2018

The authors wish to make the following corrections to their paper. [...]
Wasabia koreana Nakai: A Preliminary Study on Nutrients and Chemical Compounds That May Impact Sensory Properties
Authors: Kim Da-Som , Kim Hoe Sung , Lee Jookyeong , Pan Jeong Hoon , Kim Young Jun , Kim Jae Kyeom , Woo Seongmin , Shin Eui-Cheol
Published: October 2018

In this study, the nutritional, functional, and chemical measurements of sensory attributes of different parts of wasabi, namely, leaf, petiole, and rhizome, were investigated. Proximate composition analysis showed the presence of high amounts of carbohydrates in the rhizome and amino acid composition analysis confirmed high proportions of glutamic acid and aspartic acid in all three parts. While proximate composition showed low lipid content in wasabi, ω-3 fatty acids accounted for a high proportion (>44%) of the total lipids. Wasabi leaves had high vitamin C and total phenolic contents, and thus demonstrated antioxidant capacity. Allyl isothiocyanate, which gives wasabi its characteristic pungent taste, was identified by gas chromatography/mass spectrometry and an electronic nose. On an electronic tongue, wasabi leaves showed compounds associated with sourness and saltiness while the petiole had high content of compounds associated with sweetness and bitterness. This study provides basic data for the utilization of wasabi parts as food materials based on their nutritional, functional, and chemical measure of sensory attributes.
Chemical Composition, Antioxidant Activity and α-Glucosidase Inhibitory Activity of Chaenomeles Speciosa from Four Production Areas in China
Authors: Zheng Xuan , Wang Hongwei , Zhang Peng , Gao Lin , Yan Ning , Li Panpan , Liu Xinmin , Du Yongmei , Shen Guoming
Published: October 2018

Chaenomeles speciosa (Sweet) Nakai is a medicinal plant. Until date, there are no studies focusing on comparing the chemical profiles, antioxidant activity and α-glucosidase inhibitory activity of the dried fruits of C. speciosa from different production regions. In the study, we investigated the chemical components of dried fruits of C. speciosa from Yunnan, Chongqing, Zhejiang and Anhui provinces in China in relation to the antioxidant activity and α-glucosidase inhibitory activity. C. speciosa from Yunnan had higher total flavonoid (47.92 ± 3.79 mg/g), total polyphenol (29.15 ± 0.29 mg/g) and polysaccharide (27.60 ± 1.56 mg/g) contents than plants from other production areas. Samples from Yunnan, Zhejiang and Anhui (all > 3200 mg/kg) had higher free amino acid contents than those from Chongqing (2286.66 mg/kg). Oleanolic acid and ursolic acid levels were highest in samples from Zhejiang (555.98 ± 20.88 μg/g) and Anhui (321.06 ± 14.64 μg/g), respectively. C. speciosa from Chongqing had low total flavonoid, total polyphenol, polysaccharide, free amino acid, oleanolic acid and ursolic acid contents but high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g). Among four production areas, Yunnan represented the highest antioxidant activity and α-glucosidase inhibitory activity. In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant. These results would be helpful for evaluating the quality of C. speciosa in the different production areas.
Dominant Role of Young’s Modulus for Electric Power Generation in PVDF–BaTiO3 Composite-Based Piezoelectric Nanogenerator
Authors: Kim Hyun Soo , Lee Dong Woo , Kim Do Hyung , Kong Dae Sol , Choi Jinhyeok , Lee Minbaek , Murillo Gonzalo , Jung Jong Hoon
Published: October 2018

The electric power output of a piezoelectric nanogenerator (PENG) depends on the various physical parameters of the constituent materials, including the piezoelectric coefficient, Young’s modulus, and dielectric constant. Herein, we report the mechanical and electrical properties of a poly(vinylidene fluoride)–BaTiO3 (PVDF–BTO) composite-based PENG. Variation of the BTO nanoparticle (NP) content enabled the systematic tuning of the physical parameters that are related to power generation in the composite. The Young’s modulus of the PVDF–BTO composite initially increased, and then eventually decreased, with the increasing BTO content, which was probably due to the clustering effect of the high modulus BTO NPs. The dielectric constant of the composite continuously increased as the BaTiO3 content increased. The piezoelectric outputs were greatly enhanced at 10 wt% of BTO, where the Young’s modulus was the highest. These results indicate that the Young’s modulus plays an important role in the piezoelectric power generation of the composite-based PENGs.
Fluorescence Tumor-Imaging Using a Thermo-Responsive Molecule with an Emissive Aminoquinoline Derivative
Authors: Araki Takeru , Fuchi Yasufumi , Murayama Shuhei , Shiraishi Ryoma , Oyama Tokimi , Aso Mariko , Aoki Ichio , Kobayashi Shigeki , Yamada Ken-ichi , Karasawa Satoru
Published: October 2018

We synthesized (2,4-trifluoromethyl-7-N-bis(2,5,8,11-tetraoxatridecane-13-yl)-aminoquinoline) TFMAQ-diEg4, an emissive aminoquinoline derivative that incorporated two tetraethyleneglycol chains into an amino group. TFMAQ-diEg4 showed fluorescence and thermo-responsive properties accompanied by a lower critical solution temperature (LCST), due to the introduction of the oligoethylene glycol chain. This thermo-responsive LCST behavior occurred at the border of a cloud point. Below and above the cloud point, self-assemblies of 6-7-nm nanoparticles and ~2000-nm microparticles were observed, in vitro. In addition, TFMAQ-diEg4 showed a high solubility, over 20 mM for aqueous solution, in vivo, which not only prevented thrombosis but also allowed various examinations, such as single intravenous administration and intravenous drips. Intravenous administration of TFMAQ-diEg4, to tumor-bearing, mice led to the accumulation of the molecule in the tumor tissue, as observed by fluorescence imaging. A subset of mice was treated with local heat around their tumor tissue and an intravenous drip of TFMAQ-diEg4, which led to a high intensity of TFMAQ-diEg4 emission within the tumor tissue. Therefore, we revealed that TFMAQ-diEg4 was useful as a fluorescence probe with thermo-responsive properties.
Emerging Functions for Cell Wall Polysaccharides Accumulated during Eudicot Seed Development
Authors: Sechet Julien , Marion-Poll Annie , North Helen M.
Published: October 2018

The formation of seeds is a reproductive strategy in higher plants that enables the dispersal of offspring through time and space. Eudicot seeds comprise three main components, the embryo, the endosperm and the seed coat, where the coordinated development of each is important for the correct formation of the mature seed. In addition, the seed coat protects the quiescent progeny and can provide transport mechanisms. A key underlying process in the production of seed tissues is the formation of an extracellular matrix termed the cell wall, which is well known for its essential function in cytokinesis, directional growth and morphogenesis. The cell wall is composed of a macromolecular network of polymers where the major component is polysaccharides. The attributes of polysaccharides differ with their composition and charge, which enables dynamic remodeling of the mechanical and physical properties of the matrix by adjusting their production, modification or turnover. Accordingly, the importance of specific polysaccharides or modifications is increasingly being associated with specialized functions within seed tissues, often through the spatio-temporal accumulation or remodeling of particular polymers. Here, we review the evolution and accumulation of polysaccharides during eudicot seed development, what is known of their impact on wall architecture and the diverse roles associated with these in different seed tissues.
Conformation Study of Dual Stimuli-Responsive Core-Shell Diblock Polymer Brushes
Authors: Chen Kaimin , Cao Lan , Zhang Ying , Li Kai , Qin Xue , Guo Xuhong
Published: October 2018

Stimuli-responsive nanoparticles are among the most popular research topics. In this study, two types of core-shell (polystyrene with a photoiniferter (PSV) as the core and diblock as the shell) polymer brushes (PSV@PNIPA-b-PAA and PSV@PAA-b-PNIPA) were designed and prepared using surface-initiated photoiniferter-mediated polymerization (SI-PIMP). Moreover, their pH- and temperature-stimuli responses were explored by dynamic light scattering (DLS) and turbidimeter under various conditions. The results showed that the conformational change was determined on the basis of the competition among electrostatic repulsion, hydrophobic interaction, hydrogen bonding, and steric hindrance, which was also confirmed by protein adsorption experiments. These results are not only helpful for the design and synthesis of stimuli-responsive polymer brushes but also shed light on controlled protein immobilization under mild conditions.
Kinetic, Isotherm, and Thermodynamic Studies for Ag(I) Adsorption Using Carboxymethyl Functionalized Poly(glycidyl methacrylate)
Authors: Zhao Jiling , Wang Shixing , Zhang Libo , Wang Chen , Zhang Bing
Published: October 2018

Industrial wastewater contains large amounts of silver ions. Here, a new adsorbent was synthesized by functionalizing poly(glycidyl methacrylate) with carboxymethyl groups. The adsorbent was used to recover Ag(I) in wastewater. Fourier transform infrared spectroscopy, zeta potential, scanning electron microscopy, and X-ray photoelectron spectroscopy were used to characterize the adsorbent. The experimental parameters affecting the adsorption are solution pH, contact time, and initial silver ion concentration. The optimum pH for adsorption of Ag(I) is pH 4. The maximum adsorption capacity at pH 4 is 157.05 mg/g, and the adsorption reaches equilibrium at 300 min. The kinetics and isotherms of the adsorption process were described by pseudo second-order, Langmuir and D-R models, respectively. The adsorption process was a single layer chemical adsorption, exothermic, feasible, and spontaneous. The adsorption mechanism is electrostatic or chelation. The adsorbent selectively absorbed Ag(I) from coexisting ions (Cu2+, Ni2+, Co2+, Zn2+). Finally, the removal rate of silver ions decreased from 79.29% to 65.01% after four repetitive experiments, which proved that the adsorbent had good reusability. The adsorbent has great potential benefit in removing Ag(I).
Religiosity and Informal Economic Practices in Southeastern European Societies
Authors: Krstić Nemanja , Dinić Jelena , Gavrilović Danijela
Published: October 2018

The dominant religions in Southeastern European countries (Serbia, Macedonia, Montenegro, Bosnia and Herzegovina, Kosovo, Albania), Orthodoxy, Islam, and Catholicism, contain social teachings, which include several norms that deal with certain forms of economic practices. These post-socialist societies develop various forms of informal practices, some of which are contrary to elements of religious social teachings and religious ethics. In the process of the revitalization of religiosity after the fall of socialism in this region, the question can be posed as to whether the attitude towards informality and the application of certain informal economic practices, which range from the illegitimate to the illegal (getting things “done” through informal connections, tax evasion, corruption), correlates to some extent with the level of religiosity and the type of religion. The results of the research show that there is a connection between belonging to a certain confession or religion, self-declared religiosity and level of religiosity, and approving of informal practices and engaging in them. At the state level, a specific dynamic was developed even when it came to approving of and engaging in informal practices depending on whether the members of certain confessions were a minority or a majority at the level of the observed country.