bluuebunny/crossref_metadata_2025 huggingface dataset:
The power of three-dimensional printing technology in functional restoration of rare maxillomandibular deformity due to genetic disorder: a case report
Authors: Oren Daniel , Dror Amiel A. , Bramnik Tania , Sela Eyal , Granot Igal , Srouji Samer
Published: April 2021

Background

Thalassemia is an inherited autosomal recessive blood disorder causing abnormal formation of hemoglobin, known as a syndrome of anemia with microcytic erythrocytes. It is the most common genetic disorder worldwide, with a high prevalence among individuals of Mediterranean descent. The state of homozygosity of the beta-globin mutated gene is known as beta-thalassemia major, and these patients require regular blood transfusions and iron chelation therapy for survival. The rapid loss of red blood cells among affected individuals activates compensatory mechanisms of excessive medullary and extramedullary hematopoiesis, leading to severe skeletal bone deformity.

Case presentation

We present the case of a 39-year-old Bedouin male, diagnosed with beta-thalassemia major at infancy, with diagnosed homozygosity for the intervening sequence 2-1 (guanine > adenine) mutation. Since early infancy, he started receiving blood transfusions with a gradual increase in treatment frequency through adulthood due to the severe clinical progression of the disease. He was referred to the oral and maxillofacial surgery department at Galilee Medical Center to evaluate his facial deformity in the upper jaw and treat his severe periodontal disease. The patient presented maxillary overgrowth, and severe dental deformity resulted in progressive disfigurement and difficulty chewing, swallowing, and speaking. To address the challenge of surgical treatment, we utilized the advantage of three-dimensional planning and printing technology to simulate the optimal result. Resection of maxillary bone overgrowth and insertion of custom-made subperiosteal implants were followed by rehabilitation of both jaws to the patients' satisfaction at 3-year follow-up.

Conclusions

The ongoing implementation of state-of-the-art technologies such as virtual reality and three-dimensional printing has become a prominent component in surgical toolsets. Comprehensive case simulation and accurate planning before surgery will improve surgical results and patient satisfaction. This approach is highly advocated when approaching a case of rare maxillofacial deformity associated with either genetic or orphan diseases.
Hidradenoma papilliferum of the hymen: a case report
Authors: Birge Ozer , Bakır Mehmet Sait , Karadag Ceyda , Eldarova Zivar , Simsek Tayup
Published: April 2021

Background

Hidradenoma papilliferum is a rare benign neoplasm arising from apocrine glands. It occurs commonly on the anogenital region of middle-aged women. It usually presents as a slow growing, solitary asymptomatic, skin colored or red nodule less than 1 cm in diameter.

Case presentation

The case is a 38-year-old, white woman who presented with a painful nodule occurring within a month in the himenal region of the posterior vaginal introitus. The nodule was excisied and the histology revealed a hidradenoma papilliferum. The diagnosis and treatment of hidradenoma papilliferum is possible with surgical removal and histopathological evaluation of nodules.

Conclusion

When an adult woman presents with a noduler lesion in the anogenital area, sexually transmitted diseases and other benign and malignant vulvar lesions, as well as malignant transformation is very rare but,should be kept in mind; however because it has been reported and long-term clinical follow-up is suggested
Extracellular vesicles derived from umbilical cord mesenchymal stromal cells alleviate pulmonary fibrosis by means of transforming growth factor-β signaling inhibition
Authors: Shi Liyan , Ren Jing , Li Jiping , Wang Dongxu , Wang Yusu , Qin Tao , Li Xiuying , Zhang Guokun , Li Chunyi , Wang Yimin
Published: April 2021

Background

Pulmonary fibrosis (PF), the end point of interstitial lung diseases, is characterized by myofibroblast over differentiation and excessive extracellular matrix accumulation, leading to progressive organ dysfunction and usually a terminal outcome. Studies have shown that umbilical cord-derived mesenchymal stromal cells (uMSCs) could alleviate PF; however, the underlying mechanism remains to be elucidated.

Methods

The therapeutic effects of uMSC-derived extracellular vesicles (uMSC-EVs) on PF were evaluated using bleomycin (BLM)-induced mouse models. Then, the role and mechanism of uMSC-EVs in inhibiting myofibroblast differentiation were investigated in vivo and in vitro.

Results

Treatment with uMSC-EVs alleviated the PF and enhanced the proliferation of alveolar epithelial cells in BLM-induced mice, thus improved the life quality, including the survival rate, body weight, fibrosis degree, and myofibroblast over differentiation of lung tissue. Moreover, these effects of uMSC-EVs on PF are likely achieved by inhibiting the transforming growth factor-β (TGF-β) signaling pathway, evidenced by decreased expression levels of TGF-β2 and TGF-βR2. Using mimics of uMSC-EV-specific miRNAs, we found that miR-21 and miR-23, which are highly enriched in uMSC-EVs, played a critical role in inhibiting TGF-β2 and TGF-βR2, respectively.

Conclusion

The effects of uMSCs on PF alleviation are likely achieved via EVs, which reveals a new role of uMSC-EV-derived miRNAs, opening a novel strategy for PF treatment in the clinical setting.
Human bone marrow-derived stromal cell behavior when injected directly into the bone marrow of NOD-scid-gamma mice pre-conditioned with sub-lethal irradiation
Authors: Nowlan Bianca , Futrega Kathryn , Williams Elizabeth Deborah , Doran Michael Robert
Published: April 2021

Background

Direct bone marrow injection of cells into murine marrow cavities is used in a range of cell characterization assays and to develop disease models. While human bone marrow-derived stromal cells (hBMSC, also known as mesenchymal stem cells (MSC)) are frequently described in therapeutic applications, or disease modeling, their behavior following direct injection into murine bone marrow is poorly characterized. Herein, we characterized hBMSC engraftment and persistence within the bone marrow of NOD-scidinterleukin (IL)-2γ−/−(NSG) mice with or without prior 2 Gy total-body γ-irradiation of recipient mice.

Methods

One day after conditioning NSG mice with sublethal irradiation, 5 × 105luciferase (Luc) and green fluorescent protein (GFP)-expressing hBMSC (hBMSC-Luc/GFP) were injected into the right femurs of animals. hBMSC-Luc/GFP were tracked in live animals using IVIS imaging, and histology was used to further characterize hBMSC location and behavior in tissues.

Results

hBMSC-Luc/GFP number within injected marrow cavities declined rapidly over 4 weeks, but prior irradiation of animals delayed this decline. At 4 weeks, hBMSC-Luc/GFP colonized injected marrow cavities and distal marrow cavities at rates of 2.5 ± 2.2% and 1.7 ± 1.9% of total marrow nucleated cells, respectively in both irradiated and non-irradiated mice. In distal marrow cavities,  hBMSC were not uniformly distributed and appeared to be co-localized in clusters, with the majority found in the endosteal region.

Conclusions

While significant numbers of hBMSC-Luc/GFP could be deposited into the mouse bone marrow via direct bone marrow injection, IVIS imaging indicated that the number of hBMSC-Luc/GFP in that bone marrow cavity declined with time. Irradiation of mice prior to transplant only delayed the rate of hBMSC-Luc/GFP population decline in injected femurs. Clusters of hBMSC-Luc/GFP were observed in the histology of distal marrow cavities, suggesting that some transplanted cells actively homed to distal marrow cavities. Individual cell clusters may have arisen from discrete clones that homed to the marrow, and then underwent modest proliferation. The transient high-density population of hBMSC within the injected femur, or the longer-term low-density population of hBMSC in distal marrow cavities, offers useful models for studying disease or regenerative processes. Experimental designs should consider how relative hBMSC distribution and local hBMSC densities evolve over time.
Farnesoid X receptor via Notch1 directs asymmetric cell division of Sox9+ cells to prevent the development of liver cancer in a mouse model
Authors: Chen Mi , Lu Chenxia , Lu Hanwen , Zhang Junyi , Qin Dan , Liu Shenghui , Li Xiaodong , Zhang Lisheng
Published: April 2021

Background

Asymmetrical cell division (ACD) maintains the proper number of stem cells to ensure self-renewal. The rate of symmetric division increases as more cancer stem cells (CSCs) become malignant; however, the signaling pathway network involved in CSC division remains elusive. FXR (Farnesoid X receptor), a ligand-activated transcription factor, has several anti-tumor effects and has been shown to target CSCs. Here, we aimed at evaluating the role of FXR in the regulation of the cell division of CSCs.

Methods

The FXR target gene and downstream molecular mechanisms were confirmed by qRT-PCR, Western blot, luciferase reporter assay, EMAS, Chip, and IF analyses. Pulse-chase BrdU labeling and paired-cell experiments were used to detect the cell division of liver CSCs. Gain- and loss-of-function experiments in Huh7 cells and mouse models were performed to support findings and elucidate the function and underlying mechanisms of FXR-Notch1 in liver CSC division.

Results

We demonstrated that activation of Notch1 was significantly elevated in the livers of hepatocellular carcinoma (HCC) in Farnesoid X receptor-knockout (FXR-KO) mice and that FXR expression negatively correlated with Notch1 level during chronic liver injury. Activation of FXR induced the asymmetric divisions of Sox9+ liver CSCs and ameliorated liver injury. Mechanistically, FXR directs Sox9+ liver CSCs from symmetry to asymmetry via inhibition of Notch1 expression and activity. Deletion of FXR signaling or over-expression of Notch1 greatly increased Notch1 expression and activity along with ACD reduction. FXR inhibited Notch1 expression by directly binding to its promoter FXRE. FXR also positively regulated Numb expression, contributing to a feedback circuit, which decreased Notch1 activity and directed ACD.

Conclusion

Our findings suggest that FXR represses Notch1 expression and directs ACD of Sox9+ cells to prevent the development of liver cancer.
Angiogenesis imaging study using interim [18F] RGD-K5 PET/CT in patients with lymphoma undergoing chemotherapy: preliminary evidence
Authors: Tonnelet David , Bohn M. D. Pierre , Becker Stephanie , Decazes Pierre , Camus Vincent , Thureau Sebastien , Tilly Hervé , Jardin Fabrice , Vera Pierre
Published: April 2021

Background

Our aim was to measure the impact of two cycles of standard chemotherapy on tumoural neoangiogenesis by [18F] fluorine arginine-glycine-aspartic (RGD-K5) positron emission tomography–computed tomography (PET) on patients presenting with lymphoma. Nineteen patients at Rouen’s Henri Becquerel Cancer Centre were prospectively included. Fluorodeoxyglucose (FDG) and RGD-K5 PET were performed before (C0) and after (C2) two cycles of chemotherapy. End-of-treatment FDG PET was performed for final evaluation. Maximum standardised uptake value (SUVmax), SUVmean, Metabolic Tumour Volume (MTV) and Angiogenic Tumour Volume (ATV) were measured for all lesions. RGD SUVmax and SUVmean were also analysed in 13 normal organs at C0 and C2. The patient’s treatment response was considered using the Deauville score (DS) at the end of FDG PET treatment (DS 1–3 were considered responders, and 4 and 5 non-responders).

Results

Eighteen patients had both C0 FDG and RGD PET. Twelve patients had both C2 FDG and RGD, completed the treatment protocol and were included in end-of-treatment analysis. No statistical difference was found in RGD uptake of normal organs before and after chemotherapy for SUVmax and SUVmean. On C0 RGD, apart from classical Hodgkin lymphoma (cHL; n = 5) and grey zone lymphoma (GZL; n = 1), other lymphoma sub-types (n = 12) had low RGD uptake (p < 0.001). Regarding FDG, there was no significant difference for SUVmax, SUVmean and MTV at C0 and C2 between patients with cHL and non-Hodgkin lymphoma (NHL). At C2 RGD, non-responders had higher SUVmax and SUVmean compared to responders (p < 0.001). There was no significant difference in RGD ATV between responders and non-responders.

Conclusions

Our study showed significant higher initial RGD uptake in patients presenting with cHL and GZL compared to NHL. Non-responder also had higher post-chemotherapy RGD uptake compared to responders. Issues raised by RGD uptake, particularly in cHL, are yet to be explored and need to be confirmed in a larger population.
Effectiveness of community-based interventions for patients with schizophrenia spectrum disorders: a study protocol for a systematic review
Authors: Kim Soo-Yeon , Kim Ah. Rim
Published: April 2021

Background

Schizophrenia requires a community-based intervention approach combined with standard treatment to prevent relapses. A literature review is required to understand the effectiveness of community-based interventions and to enhance quality in countries where they have not been fully established. This is a protocol for a systematic review of the effectiveness of community-based interventions for patients with schizophrenia spectrum disorders.

Methods

We will search (from inception to January 2021) PubMed/MEDLINE, EMBASE, PsycINFO, CENTRAL, CINAHL, and Research Information Sharing Service/Korean databases. Randomized controlled trials on community-based interventions for patients with schizophrenia spectrum disorders will be eligible. The comparison groups will include patients with schizophrenia spectrum disorders who are only receiving the usual care and those who also receive community-based interventions. The schizophrenia spectrum disorders referred to in this study are defined according to the DSM-5: delusional disorders, schizophrenic disorders, and schizoaffective disorder will be included. Relapse/re-hospitalization rates (primary outcome) and quality of life (secondary outcome) will be identified for each group. Two reviewers will independently screen study titles, abstract data, and full-text articles and perform the data extraction process. Potential conflicts will be resolved through discussion. The study risk of bias will be appraised using the Cochrane Risk of Bias 2.0 tool. Results will be descriptively synthesized and will be structured according to patients’ characteristics, intervention type and exposure, and outcome type. If feasible and appropriate, outcome data will be used to perform random effects meta-analyses. Discrete variables will be calculated via odds ratio, and continuous variables will be calculated via standardized mean difference using RevMan 5.3 software.

Discussion

We will provide a summary of the available evidence on the effectiveness of community-based interventions and specific guidelines to improve their outcomes.

Systematic review registration

PROSPERO (CRD42019145660).
Targeted exome sequencing identifies mutational landscape in a cohort of 1500 Chinese patients with non-small cell lung carcinoma (NSCLC)
Authors: Zhou Ya-jun , Zheng Wei , Zeng Qing-hua , Ye Yang , Wang Ce , Fang Cheng , Liu Chao-jun , Niu Li , Wu Li-ming
Published: April 2021

Background

Non-small cell lung carcinoma (NSCLC) is one of the most common human cancers, comprising approximately 80–85% of all lung carcinomas. An estimated incidence of NSCLC is approximately 2 million new cases per year worldwide.

Results

In recent decade, the treatment of NSCLC has made breakthrough progress owing to a large number of targeted therapies which were approved for clinical use. Epidemiology, genetic susceptibility, and molecular profiles in patients are likely to play an important factor in response rates and survival benefits to these targeted treatments and thus warrant further investigation on ethnic differences in NSCLC. In this study, a total number of 1500 Chinese patient samples,1000 formalin fixed paraffin-embedded (FFPE) and 500 blood samples, from patients with NSCLC were analyzed by targeted sequencing to explore mutational landscape in ethnic groups associated with China.

Conclusions

Overall, the data presented here provide a comprehensive analysis of NSCLC mutational landscape in Chinese patients and findings are discussed in the context of similar studies on different ethnic groups.
Learner-content interaction in e-learning- the moderating role of perceived harm of COVID-19 in assessing the satisfaction of learners
Authors: Kumar Pardeep , Saxena Charu , Baber Hasnan
Published: April 2021

Envisioning learning sans interaction is absurd. Interaction plays a pivotal role in the efficacy and effectiveness of the present-day blended learning systems. Learner-content interaction contributes predominately towards the successful realization of the expected learning outcomes. In order to satisfy the learners and to impart them quality knowledge and education, e-learning content comprising of excellent learning and website content is of paramount importance. In the present COVID-19 outbreak challenging times the significance of e-learning system development and its application is much more pronounced. To gauge this, the study aims to examine the relationship between learner-content and the e-learning quality to determine the impact of e-learning quality on learners’ satisfaction under the moderating effect of perceived harm due to COVID-19. A structured questionnaire was used to gather data from 435 graduate and undergraduate management students (International and national) in Indian Universities. Findings indicate statistically significant relationships between the e-learning content and e-learning quality and; e-learning quality and the students’ satisfaction. The perceived harm has an insignificant moderating effect on students’ satisfaction. The results of the study further depict that the quality of e-learning has a significant positive relationship with the students’ satisfaction, and this relationship is not affected by the threat of being infected on the campus during the pandemic of COVID-19. To achieve the learners’ satisfaction, the institutions should strive for rendering the e-learning content of supreme quality. The mediating role of e-learning quality between content and students’ satisfaction is also established to be a significant one.
Carbohydrate-binding modules targeting branched polysaccharides: overcoming side-chain recalcitrance in a non-catalytic approach
Authors: Liu Jiawen , Sun Di , Zhu Jingrong , Liu Cong , Liu Weijie
Published: April 2021

Extensive decoration of backbones is a major factor resulting in resistance of enzymatic conversion in hemicellulose and other branched polysaccharides. Employing debranching enzymes is the main strategy to overcome this kind of recalcitrance at present. A carbohydrate-binding module (CBM) is a contiguous amino acid sequence that can promote the binding of enzymes to various carbohydrates, thereby facilitating enzymatic hydrolysis. According to previous studies, CBMs can be classified into four types based on their preference in ligand type, where Type III and IV CBMs prefer to branched polysaccharides than the linear and thus are able to specifically enhance the hydrolysis of substrates containing side chains. With a role in dominating the hydrolysis of branched substrates, Type III and IV CBMs could represent a non-catalytic approach in overcoming side-chain recalcitrance.
Chinese expert consensus on diagnosis and treatment of trauma-induced hypercoagulopathy
Authors:   Chinese People’s Liberation Army Professional Committee of Critical Care Medicine and Chinese Society of Thrombosis, Hemostasis and Critical Care, Chinese Medicine Education Association, Song Jing-Chun , Yang Li-Kun , Zhao Wei , Zhu Feng , Wang Gang , Chen Yao-Peng , Li Wei-Qin
Published: April 2021

Trauma-induced coagulopathy (TIC) is caused by post-traumatic tissue injury and manifests as hypercoagulability that leads to thromboembolism or hypocoagulability that leads to uncontrollable massive hemorrhage. Previous studies on TIC have mainly focused on hemorrhagic coagulopathy caused by the hypocoagulable phenotype of TIC, while recent studies have found that trauma-induced hypercoagulopathy can occur in as many as 22.2–85.1% of trauma patients, in whom it can increase the risk of thrombotic events and mortality by 2- to 4-fold. Therefore, the Chinese People’s Liberation Army Professional Committee of Critical Care Medicine and the Chinese Society of Thrombosis, Hemostasis and Critical Care, Chinese Medicine Education Association jointly formulated this Chinese Expert Consensus comprising 15 recommendations for the definition, pathophysiological mechanism, assessment, prevention, and treatment of trauma-induced hypercoagulopathy.
A case of pericarditis caused by Mycoplasma hyorhinis in a weaned piglet
Authors: Ustulin Martina , Rossi Erica , Vio Denis
Published: April 2021

Background

Mycoplasma hyorhinis (M. hyorhinis) is a bacterium commonly found in the upper respiratory tract of healthy pigs and an agent of polyserositis and polyarthritis. Moreover, it can carry antibiotic resistance genes (Wu et al, Vet. Microbiol. 76: 25–30, 2000). Economic losses caused by M. hyorhinis can be reduced by antibiotic therapy, however, isolation and antimicrobic susceptibility profile are rarely performed.

Case presentation

The present report describes a case of pericarditis caused by M. hyorhinis in a weaned piglet with respiratory symptoms and reduced growth performance.

At post mortem examination, the main macroscopic finding was a severe fibrinous pericarditis and M. hyorhins was the only agent isolated from the pericardial fluid.

In this strain, Minimum Inhibitory Concentration (MIC) determination revealed resistance to various antimicrobial molecules such as erythromycin, tylosin and tilmicosin.

Conclusion

This paper highlights the importance of including M. hyorhins in the differential diagnosis of polyserositis in swine. Moreover, due the possible presence of multidrug resistance, the determination of antimicrobial susceptibility pattern should be performed on a regular basis.
In-vitro cytotoxic and anti-Vibrio cholerae activities of alcoholic extracts of Desmodium triflorum (L.) whole plant and Terminalia citrina (Roxb.) fruits
Authors: Karim Rubaba , Begum Mst Marium , Jui Yeasmin , Islam Tanjina , Billah Masum , Arafat Yeasir , Karim Mehnaz , Khan Ashfia Fatima , Rahman Md. Sohanur
Published: April 2021

Background

The majority of epidemicVibrio choleraestrains have become resistant to multiple antimicrobial agents for many reasons, including mutations, horizontal gene transfers, etc. Therefore, secondary means of treatment are needed to overcome this impediment in combating cholera.Desmodium triflorum (L.)andTerminalia citrina (Roxb.)are extensively used in traditional medicine. Therefore, the present study aims to evaluate the antibacterial activity of methanolic and ethanolic extracts ofDesmodium triflorum (L.)whole plant andTerminalia citrina (Roxb.)fruits againstV. choleraeisolates.

Methods

Initially, different extracts of both plant materials were subjected to qualitative phytochemical screening. Anti-V. choleraeactivities were assessed by performing MIC (minimum inhibitory concentration) test using disc diffusion method with different concentrations (1.25, 2.50, 8.00, 10.00, 12.50, 15.00 mg/ml) where standard discs of gentamycin and meropenem (10 μg/disc) were used. Further, Brine shrimp lethality bioassay was done to evaluate the extracts’ cytotoxic activities using different concentrations (800, 400, 200, 100, 50, 25, 12.5, 6.25 μg/ml) against vincristine sulphate.

Results

The preliminary screening confirms the presence of convenient phytochemical constituents like flavonoids, alkaloids, glycosides, tannins, etc., and are liable for the emergence of the antibacterial activities in different strains. The minimum inhibitory concentrations of both methanolic and ethanolic extracts ofTerminalia citrina(fruits) andDesmodium triflorum (L.)(whole plant) were found to be 2.50 mg/ml and 5 mg/ml, respectively. Moreover, 100% mortality was observed only in ethanolic extract ofTerminalia citrinafruits at the highest concentration (800 μg/ml). In comparison, the rest of the test extracts showed less than 90% mortality at the highest concentration (800 μg/ml).

Conclusions

Both plants possess significant phytoconstituents and showed appreciable anti-V. choleraeactivities with mild toxicity. Hence,Desmodium triflorum (L.)whole plant andTerminalia citrina (Roxb.)fruits may be recommended as potential phytotherapy against cholera disease.
Phytotherapeutic potential of bi-herbal extract of cinnamon and turmeric: in vivo antidiabetic studies
Authors: Sivaranjani R. , Zachariah T. John , Leela N. K.
Published: April 2021

Background

The spices, cinnamon and turmeric have both culinary and pharmaceutical applications. Earlier studies proved their effect on reducing the symptoms associated with type 2 diabetes, a major lifestyle disease affecting millions world over. In our work, we prepared a bi-herbal extract of cinnamon and turmeric and studied its effect to alleviate the symptoms of type 2 diabetes in rat model.

Method

The phenolic rich bi-herbal extract was given to diabetes induced male wistar rats for 28 days at two different concentrations (50 and 150 mg/Kg bwt.). The changes in blood glucose level were monitored at weekly interval. At the end of the experiment, blood serum was collected and used for the estimation of lipid profile, Glucose, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), Total Protein (TP), Albumin, Urea and Creatinine levels.

Result

The serum analysis revealed that administration of the bi-herbal extract at 150 mg/Kg bwt. significantly reduced the blood glucose level (152.60 ± 40.2 mg/dL; p < 0.05) of diabetic animals as compared to diabetic control (335.40 ± 67.3 mg/dL; p < 0.05) at the end of 4 weeks. The treatment also resulted in the reduction of total cholesterol and LDL-cholestrol levels as compared to diabetic control animals. The histopathological examination of tissues showed the improvement in pancreatic architecture and restoration of tissue integrity in liver and kidney.

Conclusion

The study concludes that methanol extract of combination of cinnamon and turmeric has good hypoglycemic, hypolipidemic and organ protective potential in diabetic rats which could be developed into functional food supplementation for the prevention of type 2 diabetes.
Effects of BNO 1016 on ciliary transport velocity and cell culture surface liquid height of sinonasal epithelial cultures
Authors: Workman Alan D. , Maina Ivy W. , Triantafillou Vasiliki , Patel Neil N. , Tong Charles C. L. , Kuan Edward C. , Kennedy David W. , Palmer James N. , Adappa Nithin D. , Cohen Noam A.
Published: April 2021

Background

BNO 1016 is an ethanolic extract of a mixture of five herbs that has been sold in different formulations for decades in the European market and more recently, in the United States market as an over-the-counter treatment for rhinosinusitis. Previous studies indicated activation of chloride secretion and increase in ciliary beat frequency by BNO 1016 but the functional consequences on mucociliary transport velocity and airway surface liquid homeostasis are unknown. This study intends to examine the effects of BNO 1016 on these properties in vitro.

Results

Human sinonasal epithelial cells were grown at an air-liquid interface, with addition of BNO 1016 basolaterally in each experiment. Polystyrene fluorescent microspheres were added to the apical surface of the culture, and distance traveled across the surface of the culture over a fixed time period was measured using live imaging. BNO 1016 concentrations of 50 μg/ml and 500 μg/ml were tested. Basolateral application of compound resulted in a non-dose-dependent increase in culture surface liquid height compared to controls at 30 min, and this effect persisted through the one-hour duration of the experiment (p < 0.01). Basolateral application of BNO 1016 also resulted in a non-dose-dependent increase in microsphere transport velocity at 45 and 60 min following compound application (p < 0.01).

Conclusions

Basolateral application of BNO 1016 at a concentration mimicking post-ingestion serum levels appears to elicit increases in cell culture surface liquid height and mucociliary clearance, as assessed by microsphere transport velocity. These properties can potentially be leveraged for therapeutic efficacy in diseases affecting mucus production and mucociliary transport.
New Indomalayan Nebularmis species (Heterotardigrada: Echiniscidae) provoke a discussion on its intrageneric diversity
Authors: Gąsiorek Piotr , Vončina Katarzyna , Ciosek Joanna , Veloso Mariana , Fontoura Paulo , Michalczyk Łukasz
Published: April 2021

Recent years have brought undeniable progress in tardigrade taxonomy, and speciose complexes were detected in a number of phylogenetic lineages. The family Echiniscidae is one such lineage; it is one of the most diverse groups of limno-terrestrial tardigrades and can be characterized as having achieved great evolutionary success. In this contribution, using populations representing several species that originated from the Indomalayan region, we reconstructed phylogenetic affinities within Nebularmis, a recently erected genus within the Echiniscus lineage. Nebularmis auratussp. nov. and Nebularmis burmensissp. nov. are described from the Eastern Yoma Mountains and the Shan Hills (Myanmar), Nebularmis bhutanensissp. nov. is described from the Eastern Himalayas (Bhutan), and Nebularmis indicussp. nov. is described from the foothills of the Western Ghats (Goa, India). Moreover, males are reported in populations of the last two species. All known members of the genus can be phenotypically differentiated based on minute details of their dorsal sculpture and claws. Moreover, a very wide tropical distribution is demonstrated for Nebularmis cirinoi, recorded for the first time from islands of the Malay Archipelago. Furthemore, novel morphological, genetic, and geographic data allowed for the clarification of the generic diagnosis. Currently available data favor a scenario under which Nebularmis evolved in Southeast Asia and later dispersed to other regions of the globe.
Amine oxidase copper-containing 3 (AOC3) inhibition: a potential novel target for the management of diabetic retinopathy
Authors: Boyer David S. , Rippmann Joerg F. , Ehrlich Michael S. , Bakker Remko A. , Chong Victor , Nguyen Quan Dong
Published: April 2021

Background

Diabetic retinopathy (DR), a microvascular complication of diabetes, is the leading cause of visual impairment in people aged 20–65 years and can go undetected until vision is irreversibly lost. There is a need for treatments for non-proliferative diabetic retinopathy (NPDR) which, in comparison with current intravitreal (IVT) injections, offer an improved risk–benefit ratio and are suitable for the treatment of early stages of disease, during which there is no major visual impairment. Efficacious systemic therapy for NPDR, including oral treatment, would be an important and convenient therapeutic approach for patients and physicians and would reduce treatment burden. In this article, we review the rationale for the investigation of amine oxidase copper-containing 3 (AOC3), also known as semicarbazide-sensitive amine oxidase and vascular adhesion protein 1 (VAP1), as a novel target for the early treatment of moderate to severe NPDR. AOC3 is a membrane-bound adhesion protein that facilitates the binding of leukocytes to the retinal endothelium. Adherent leukocytes reduce blood flow and in turn rupture blood vessels, leading to ischemia and edema. AOC3 inhibition reduces leukocyte recruitment and is predicted to decrease the production of reactive oxygen species, thereby correcting the underlying hypoxia, ischemia, and edema seen in DR, as well as improving vascular function.

Conclusion

There is substantial unmet need for convenient, non-invasive treatments targeting moderately severe and severe NPDR to reduce progression and preserve vision. The existing pharmacotherapies (IVT corticosteroids and IVT anti-vascular endothelial growth factor-A) target inflammation and angiogenesis, respectively. Unlike these treatments, AOC3 inhibition is predicted to address the underlying hypoxia and ischemia seen in DR. AOC3 inhibitors represent a promising therapeutic strategy for treating patients with DR and could offer greater choice and reduce treatment burden, with the potential to improve patient compliance.
18F-FACBC PET/MRI in the evaluation of human brain metastases: a case report
Authors: Johannessen Knut , Berntsen Erik Magnus , Johansen Håkon , Solheim Tora S. , Karlberg Anna , Eikenes Live
Published: April 2021

Background

Patients with metastatic cancer to the brain have a poor prognosis. In clinical practice, MRI is used to delineate, diagnose and plan treatment of brain metastases. However, MRI alone is limited in detecting micro-metastases, delineating lesions and discriminating progression from pseudo-progression. Combined PET/MRI utilises superior soft tissue images from MRI and metabolic data from PET to evaluate tumour structure and function. The amino acid PET tracer 18F-FACBC has shown promising results in discriminating high- and low-grade gliomas, but there are currently no reports on its use on brain metastases. This is the first study to evaluate the use of 18F-FACBC on brain metastases.

Case presentation

A middle-aged female patient with brain metastases was evaluated using hybrid PET/MRI with 18F-FACBC before and after stereotactic radiotherapy, and at suspicion of recurrence. Static/dynamic PET and contrast-enhanced T1 MRI data were acquired and analysed. This case report includes the analysis of four 18F-FACBC PET/MRI examinations, investigating their utility in evaluating functional and structural metastasis properties.

Conclusion

Analysis showed high tumour-to-background ratios in brain metastases compared to other amino acid PET tracers, including high uptake in a very small cerebellar metastasis, suggesting that 18F-FACBC PET can provide early detection of otherwise overlooked metastases. Further studies to determine a threshold for 18F-FACBC brain tumour boundaries and explore its utility in clinical practice should be performed.
A methodology for energy key performance indicators analysis
Authors: Faria Pedro , Lezama Fernando , Vale Zita , Khorram Mahsa
Published: April 2021

With the advent of the smart grid era, the electrical grid is becoming a complex network in which different technologies coexist to bring benefits to both customers and operators. This paper presents a methodology for analyzing Key Performance Indicators (KPIs), providing knowledge about the performance and efficiency of energy systems, focusing on the demand side. In the first stage of the methodology, the baseline KPIs are calculated. In the second stage, all KPIs are updated to be compared with the baseline ones. In fact, due to the dynamic nature of players in a smart grid, this methodology plays a crucial role in the performance assessment. Moreover, the proper definition and selection of KPIs is usually a challenging task since KPIs can be applied to evaluate diverse areas within a smart grid. Such areas include building energy efficiency, home communications, and smart metering deployment, just to mention a few. In the proposed methodology, the information obtained from a KPI can be driven to distinct aspects such as efficiency, environment, reliability, power quality, safety, security, or cost reduction. Through a case study from a real implementation of an energy system, we show how to assess energy consumption efficiency, thus improving energy management.
Industrial hemp as an agricultural crop in Ghana
Authors: Owusu Nana Osei , Arthur Benedict , Aboagye Emmanuel Mensah
Published: April 2021

Background

Cannabis is one of humanity’s oldest crops with several uses, from food to clothing and medicine. It remains one of the most controversial crops whose production, possession, and usage are regulated differently across jurisdictions. Academic research and advocacy have resulted in the redefinition of the legal status of cannabis in several countries. Ghana recently reviewed its laws on cannabis, allowing for the cultivation of industrial hemp. The legislation paves the way for Ghana to benefit from industrial hemp and include it in the agricultural cash crop list. This paper looks at the economic prospects of industrial hemp in the wake of the new law.

Methods

A systematic electronic research was conducted to identify journal articles, reports, news, blogs, and other relevant materials on cannabis, marijuana, and industrial hemp. The electronic search was done primarily on Google, Google Scholar, Bing, and “Baidu Xueshi” to identify cannabis-related publications. The search was expanded beyond Ghana to find other perspectives on cannabis. The search began in January 2020 on Google using search terms like “cannabis in Ghana” and “which countries have legal cannabis.” Materials on history, financial prospects, industrial uses, and legislations on cannabis and industrial hemp were reviewed.

Results

Existing research on cannabis in Ghana has focused on the psychotic effects of cannabis other than its industrial aspects, which has potentials for the economy. Industrial hemp has CBD with no psychotic effects and is very useful in making medicine, paper, and textiles. Ghana has both the land and workforce to produce hemp to feed local industries and the international market.

Conclusion

The new legislation can put Ghana in a position to benefit from the current cannabis industry. Therefore, policymakers should implement a registration regime that would favor local investors and farmers to reduce illegal production. The regulatory framework should establish a well-equipped agency that will supervise production and research into hemp development.
The turbidite-contourite-tidalite-baroclinite-hybridite problem: orthodoxy vs. empirical evidence behind the “Bouma Sequence”
Authors: Shanmugam G.
Published: April 2021

The underpinning problems of deep-water facies still remain unresolved. (1) The Tb, Tc, and Td divisions of the turbidite facies model, with traction structures, are an integral part of the “Bouma Sequence” (Ta, Tb, Tc, Td, Te). However, deposits of thermohaline contour currents, wind-driven bottom currents, deep-marine tidal currents, and baroclinic currents (internal waves and tides) also develop discrete rippled units, mimicking Tc. (2) The application of “cut-out” logic of sequences, which was originally introduced for the “Bouma Sequence”, with sharp basal contacts and sandy divisions containing well-developed traction structures, to muddy contourites with gradational basal contacts and an absence of well-developed traction structures is incongruent. (3) The presence of five internal divisions and hiatus in the muddy contourite facies model is in dispute. (4) Intersection of along slope contour currents with down slope sediment-gravity flows, triggering hybrid flows, also develops traction structures. (5) The comparison of genuine hybrid flows with down slope flow transformation of gravity flows is inconsistent with etymology of the term “hybrid”. (6) A reexamination of the Annot Sandstone at the Peira Cava type locality in SE France fails to validate either the orthodoxy of five internal divisions of the “Bouma Sequence” or their origin by turbidity currents. For example, the “Ta” division is composed of amalgamated units with inverse grading and floating mudstone clasts, suggesting a mass-transport deposit (MTD). The “Tb” and “Tc” divisions are composed of double mud layers and sigmoidal cross bedding, respectively, which suggest a tidalite origin. (7) Although it was reasonable to introduce a simplistic “Bouma Sequence” in 1962, at a time of limited knowledge on deep-water processes, it is obsolete now in 2021 to apply this model to the rock record amid a wealth of new knowledge. (8) The disconnect between 12 observed, but questionable, modern turbidity currents and over 10,000 interpreted ancient turbidites defies the doctrine of uniformitarianism. This disconnect is attributed to routine application of genetic facies models, without a pragmatic interpretation of empirical data. (9) A suggested solution to these problems is to interpret traction structures in the sedimentary record pragmatically on the basis of empirical field and experimental evidence, without any built-in bias using facies models, such as the “Bouma Sequence”. (10) Until reliable criteria are developed to distinguish traction structures of each type of bottom currents based on uniformitarianism, a general term “BCRS” (i.e., bottom-current reworked sands) is appropriate for deposits of all four kinds of bottom currents.
Unified gas-kinetic wave-particle methods IV: multi-species gas mixture and plasma transport
Authors: Liu Chang , Xu Kun
Published: April 2021

In this paper, we extend the unified gas-kinetic wave-particle (UGKWP) methods to the multi-species gas mixture and multiscale plasma transport. The construction of the scheme is based on the direct modeling on the mesh size and time step scales, and the local cell’s Knudsen number determines the flow physics. The proposed scheme has the multiscale and asymptotic complexity diminishing properties. The multiscale property means that according to the cell’s Knudsen number the scheme can capture the non-equilibrium flow physics when the cell size is on the kinetic mean free path scale, and preserve the asymptotic Euler, Navier-Stokes, and magnetohydrodynamics (MHD) when the cell size is on the hydrodynamic scale and is much larger than the particle mean free path. The asymptotic complexity diminishing property means that the total degrees of freedom of the scheme reduce automatically with the decreasing of the cell’s Knudsen number. In the continuum regime, the scheme automatically degenerates from a kinetic solver to a hydrodynamic solver. In the UGKWP, the evolution of microscopic velocity distribution is coupled with the evolution of macroscopic variables, and the particle evolution as well as the macroscopic fluxes is modeled from a time accumulating solution of kinetic scale particle transport and collision up to a time step scale. For plasma transport, the current scheme provides a smooth transition from particle-in-cell (PIC) method in the rarefied regime to the magnetohydrodynamic solver in the continuum regime. In the continuum limit, the cell size and time step of the UGKWP method are not restricted by the particle mean free path and mean collision time. In the highly magnetized regime, the cell size and time step are not restricted by the Debye length and plasma cyclotron period. The multiscale and asymptotic complexity diminishing properties of the scheme are verified by numerical tests in multiple flow regimes.
Effects of posterior tibial slope on the mid-term results of medial unicompartmental knee arthroplasty
Authors: Chen Zhijie , Chen Kaizhe , Yan Yufei , Feng Jianmin , Wang Yi , Liu Zhihong , Yang Qingming , He Chuan
Published: April 2021

Objective

To evaluate the effect of medial posterior tibial slope (PTS) on mid-term postoperative range of motion (ROM) and functional improvement of the knee after medial unicompartmental knee arthroplasty (UKA).

Methods

Medical records of 113 patients who had undergone 124 medial UKAs between April 2009 through April 2014 were reviewed retrospectively. The mean follow-up lasted 7.6 years (range, 6.2–11.2 years). Collected were demographic data, including gender, age, height, weight of the patients. Anteroposterior (AP) and lateral knee radiographs of the operated knees were available in all patients. The knee function was evaluated during office follow-up or hospital stay. Meanwhile, postoperative PTS, ROM, maximal knee flexion and Hospital for Special Surgery (HSS) knee score (pre−/postoperative) of the operated side were measured and assessed. According to the size of the PTS, patients were divided into 3 groups: group 1 (<4°), group 2 (4° ~ 7°) and group 3 (>7°). The association between PTS and the knee function was investigated.

Results

In our cohort, the average PTS was 2.7° ± 0.6° in group 1, 5.6° ± 0.9° in group 2 and 8.7° ± 1.2° in group 3. Pairwise comparisons showed significant differences among them (p < 0.01). The average maximal flexion range of postoperative knees in each group was 112.4° ± 5.6°, 116.4° ± 7.2°, and 117.5° ± 6.1°, respectively, with significant difference found between group 1 and group 2 (p < 0.05), and between group 1 and group 3 (p < 0.05). However, the gender, age, and body mass index (BMI) did not differ between three groups and there was no significant difference between groups in terms of pre−/postoperative HSS scores or postoperative knee ROM.

Conclusion

A mid-term follow-up showed that an appropriate PTS (4° ~ 7°) can help improve the postoperative flexion of knee. On the other hand, too small a PTS could lead to limited postoperative knee flexion. Therefore, the PTS less than 4° should be avoided during medial UKA.
Implementing immediate postpartum contraception: a comparative case study at 11 hospitals
Authors: Moniz Michelle H. , Bonawitz Kirsten , Wetmore Marisa K. , Dalton Vanessa K. , Damschroder Laura J. , Forman Jane H. , Peahl Alex F. , Heisler Michele
Published: April 2021

Background

Immediate postpartum long-acting reversible contraception (LARC) is an evidence-based practice, but hospitals face significant barriers to its adoption. Our objective was to examine how organizational context (e.g., size, employee attitudes toward the clinical practice) and implementation strategies (i.e., the actions taken to routinize a clinical practice) drive successful implementation of immediate postpartum LARC services, with a goal of informing the design of future implementation interventions.

Methods

We conducted a comparative case study of the implementation of inpatient postpartum contraceptive care at 11 US maternity hospitals. In 2017–2018, we conducted site visits that included semi-structured key informant interviews informed by the Consolidated Framework for Implementation Research. Qualitative measures of implementation success included stakeholder satisfaction, routinization, and sustainability of immediate postpartum LARC services. Qualitative content analysis and cross-case synthesis explored relationships among organizational context, implementation strategies, and implementation success.

Results

We completed semi-structured interviews with 78 clinicians, nurses, residents, pharmacy and revenue cycle staff, and hospital administrators. Successful implementation required three essential conditions: effective implementation champions, an enabling financial environment, and hospital administrator engagement. Six other contextual conditions were influential: trust and effective communication, alignment with stakeholders’ professional values, perception of meeting patients’ needs, robust learning climate, compatibility with workflow, and positive attitudes and adequate knowledge about the clinical practice. On average, sites used 18 (range 11-22) strategies. Strategies to optimize the financial environment and train clinicians and staff were commonly used. Strategies to plan and evaluate implementation and to engage patients emerged as promising to address barriers to practice change, yet were often underused.

Conclusions

Implementation efforts in maternity settings may be more successful if they select strategies to optimize local conditions for success. Our findings elucidate key contextual conditions to target and provide a menu of promising implementation strategies for incorporating recommended contraceptive services into routine maternity practice. Additional prospective research should evaluate whether these strategies effectively optimize local conditions for successful implementation in a variety of settings.