bluuebunny/crossref_metadata_2025 huggingface dataset:
Five Mitochondrial Genomes of the Genus Eysarcoris Hahn, 1834 with Phylogenetic Implications for the Pentatominae (Hemiptera: Pentatomidae)
Authors: Li Rongrong , Li Min , Yan Jiang , Bai Ming , Zhang Hufang
Published: June 2021

Four complete mitogenomes of Eysarcoris rosaceus, E. montivagus, E. gibbosus, E. annamita and one near-complete mitochondrial genome of E. ventralis were sequenced and used to explore the phylogenetic relationships of tribes within the subfamily Pentatominae. The mitogenomes range from 15,422 to 16,043 base pairs (bp) in length and encode 37 genes, including 13 protein-coding genes (PCGs), two rRNA genes, 22 tRNA genes (21 in E. ventralis), and a control region. Similar to other heteropteran species, the AT contents of the sequenced species were higher than their GC contents. The most frequently used start/stop codon was ATN/TAA. GTG was only found in atp6 and atp8 of E. gibbosus. All transfer RNA genes (tRNAs) exhibit the typical cloverleaf secondary structure, except for the trnS1 and trnV, which lacks the stem of the DHU arm. The length and copy number of repeat units were conserved within Eysarcoris, with the exception of E. gibbosus. Phylogenetic analyses based on mitogenomes using both maximum likelihood (ML) and Bayesian inference (BI) methods strongly supported the relationship among tribes within Pentatominae and confirmed that Graphosoma should be an intermediate lineage of Pentatominae. The relationship between Eysarcoris and Carbula was strongly supported and combined with our previous geometric morphometrics and chromosomal studies, suggest the Eysarcoris should belong to the tribe Eyasrcorini. This work will help to enhance our understanding of mitochondrial genomic evolution and phylogenetic relationships in Pentatominae.
Achievements and Challenges in Transplantation of Mesenchymal Stem Cells in Otorhinolaryngology
Authors: Kaboodkhani Reza , Mehrabani Davood , Karimi-Busheri Feridoun
Published: June 2021

Otorhinolaryngology enrolls head and neck surgery in various tissues such as ear, nose, and throat (ENT) that govern different activities such as hearing, breathing, smelling, production of vocal sounds, the balance, deglutition, facial animation, air filtration and humidification, and articulation during speech, while absence of these functions can lead to high morbidity and even mortality. Conventional therapies for head and neck damaged tissues include grafts, transplants, and artificial materials, but grafts have limited availability and cause morbidity in the donor site. To improve these limitations, regenerative medicine, as a novel and rapidly growing field, has opened a new therapeutic window in otorhinolaryngology by using cell transplantation to target the healing and replacement of injured tissues. There is a high risk of rejection and tumor formation for transplantation of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs); mesenchymal stem cells (MSCs) lack these drawbacks. They have easy expansion and antiapoptotic properties with a wide range of healing and aesthetic functions that make them a novel candidate in otorhinolaryngology for craniofacial defects and diseases and hold immense promise for bone tissue healing; even the tissue sources and types of MSCs, the method of cell introduction and their preparation quality can influence the final outcome in the injured tissue. In this review, we demonstrated the anti-inflammatory and immunomodulatory properties of MSCs, from different sources, to be safely used for cell-based therapies in otorhinolaryngology, while their achievements and challenges have been described too.
Influence of Different Thermal Aging Conditions on Soot Combustion with Catalyst by Thermogravimetric Analysis
Authors: Yang Yi , Fang Jia , Huang Junfeng , Qin Zihan , Zhang Qian , Pu Ping , Pan Suozhu
Published: June 2021

Diesel particulates are deposited in the diesel particulate filter and removed by the regeneration process. The Printex-U (PU) particles are simulated as the diesel soot to investigate the influence of thermal aging conditions on soot combustion performance with the addition of catalysts. The comprehensive combustion index S, combustion stability index Rw and peak temperature Tp are obtained to evaluate the combustion performance. Compared with the PU/Pt mixtures of different Pt contents (2 g/ft3, 3.5 g/ft3, and 5 g/ft3), the 10 g/ft3 Pt contents improve soot combustion with the outstanding oxygen absorption ability. When the weight ratio of PU/Pt mixture is 1:1, the promoted effect achieves the maximum degree. The S and Rw increase to 8.90 × 10−8 %2min−2°C−3 and 39.11 × 105, respectively, compared with pure PU. After the thermal aging process, the PU/Pt mixture with a 350 °C aging temperature for 10 h promotes the soot combustion the best when compared to pure PU particles. It is not good as the PU/Pt mixture without aging, because the inner properties of soot and Pt/Al2O3 catalyst may have been changed. The S and Rw are 9.07 × 10−8 %2min−2°C−3 and 38.39 × 105, respectively, which are close to the no aging mixture. This work plays a crucial role in understanding the mechanism of the comprehensive effect of soot and catalyst on soot combustion after the thermal aging process.
Substrate Impact on MR Characteristics of Carbon Nano Films Explored via AFM and Raman Analysis
Authors: Saleemi Awais Siddique , Hafeez Muhammad , Saeed Muhammad , Abdullah Ali , Rehman Muhammad Anis-ur- , Lee Shern-Long
Published: June 2021

Recent advances in the fabrication and classification of amorphous carbon (a-Carbon) thin films play an active part in the field of surface materials science. In this paper, a pulsed laser deposition (PLD) technique through controlling experimental parameters, including deposition time/temperature and laser energy/frequency, has been employed to examine the substrate effect of amorphous carbon thin film fabrication over SiO2 and glass substrates. In this paper, we have examined the structural and magnetoresistance (MR) properties of these thin films. The intensity ratio of the G-band and D-band (ID/IG) were 1.1 and 2.4, where the C(sp2) atomic ratio for the thin films samples that were prepared on glass and SiO2 substrates, were observed as 65% and 85%, respectively. The MR properties were examined under a magnetic field ranging from −9 T to 9 T within a 2-K to 40-K temperature range. A positive MR value of 15% was examined at a low temperature of 2 K for the thin films grown on SiO2 substrate at a growth temperature of 400 °C using a 300 mJ/pulse laser frequency. The structural changes may tune the magnetoresistance properties of these a-Carbon materials. These results were demonstrated to be highly promising for carbon-based spintronics and magnetic sensors.
Influence of Vehicle Number on the Dynamic Characteristics of High-Speed Train-CRTS III Slab Track-Subgrade Coupled System
Authors: Xu Qingyuan , Sun Hao , Wang Lexuan , Xu Lei , Chen Wei , Lou Ping
Published: June 2021

In this paper, a high-speed train–CRTS III slab track–subgrade coupled dynamic model is established. With the model, the influence of vehicle number on the dynamic characteristics of a train–CRTS III slab track–subgrade coupled system with smooth and random track irregularity conditions for conventional and vibration-reduction CRTS III slab tracks are theoretically studied and analyzed. Some conclusions are drawn from the results: (1) the largest dynamic responses of the coupled system for all items and cases are no longer changed when the vehicle number exceeds three, and three vehicles are adequate to guarantee the simulation precision to investigate the dynamic responses of the coupled system. (2) The acceleration of the car body has almost no relation with the vehicle number, and only one vehicle is needed to study the vehicle dynamics using the train–CRTS III slab track–subgrade coupled dynamic model. (3) For the conventional CRTS III slab track on a subgrade, the vehicle number has a negligible influence on the accelerations of the rail, slab, and concrete base, the positive and negative bending moments of the rail, the compressive force of the fastener, and the positive bending stress of slab, but it has a large influence on the tension force of the fastener, and the negative bending stresses of the slab and concrete base. Only one vehicle is needed to study track dynamics without considering the tension force of the fastener, the negative bending stresses of the slab and concrete base, otherwise, two or more vehicles are required. (4) For vibration reduction of the CRTS III slab track on a subgrade, the number of vehicles has some influence on the dynamic responses of all track components, and at least two vehicles are required to investigate the track dynamics.
Influence of Deposition Method on the Structural and Optical Properties of Ge2Sb2Te5
Authors: Simandan Iosif-Daniel , Sava Florinel , Buruiana Angel-Theodor , Galca Aurelian-Catalin , Becherescu Nicu , Burducea Ion , Mihai Claudia , Velea Alin
Published: June 2021

Ge2Sb2Te5 (GST-225) is a chalcogenide material with applications in nonvolatile memories. However, chalcogenide material properties are dependent on the deposition technique. GST-225 thin films were prepared using three deposition methods: magnetron sputtering (MS), pulsed laser deposition (PLD) and a deposition technique that combines MS and PLD, namely MSPLD. In the MSPLD technique, the same bulk target is used for sputtering but also for PLD at the same time. The structural and optical properties of the as-deposited and annealed thin films were characterized by Rutherford backscattering spectrometry, X-ray reflectometry, X-ray diffraction, Raman spectroscopy and spectroscopic ellipsometry. MS has the advantage of easily leading to fully amorphous films and to a single crystalline phase after annealing. MS also produces the highest optical contrast between the as-deposited and annealed films. PLD leads to the best stoichiometric transfer, whereas the annealed MSPLD films have the highest mass density. All the as-deposited films obtained with the three methods have a similar optical bandgap of approximately 0.7 eV, which decreases after annealing, mostly in the case of the MS sample. This study reveals that the properties of GST-225 are significantly influenced by the deposition technique, and the proper method should be selected when targeting a specific application. In particular, for electrical and optical phase change memories, MS is the best suited deposition method.
Wire Arc Additive Manufacturing of Al-Mg Alloy with the Addition of Scandium and Zirconium
Authors: Ponomareva Taisiya , Ponomarev Mikhail , Kisarev Arseniy , Ivanov Maxim
Published: June 2021

The proposed paper considers the opportunity of expanding the application area of wire arc additive manufacturing (WAAM) method by means of increasing the strength properties of deposited material, due to the implementation of aluminum wire with the addition of scandium and zirconium. For the experimental research, the welding wire 1575 of the Al-Mg-Sc-Zr system containing 0.23% Sc and 0.19% Zr was selected. The optimal welding parameters, ensuring the defect-free formation of deposited material with low heat input, were used. Porosity level was estimated. The thermal state was estimated by finite element simulation. Simulated thermal state was verified by comparison with thermocouples data. Post-heat treatment parameters that lead to maximum strength with good plasticity were determined. The maximum yield strength (YS) of 268 MPa and ultimate strength (UTS) of 403 MPa were obtained, while the plasticity was determined at least 16.0% in all WAAM specimens.
Untargeted Metabolomics Approach for the Discovery of Environment-Related Pyran-2-Ones Chemodiversity in a Marine-Sourced Penicillium restrictum
Authors: Le Van-Tuyen , Bertrand Samuel , Robiou du Pont Thibaut , Fleury Fabrice , Caroff Nathalie , Bourgeade-Delmas Sandra , Gentil Emmanuel , Logé Cedric , Genta-Jouve Gregory , Grovel Olivier
Published: June 2021

Very little is known about chemical interactions between fungi and their mollusc host within marine environments. Here, we investigated the metabolome of a Penicillium restrictum MMS417 strain isolated from the blue mussel Mytilus edulis collected on the Loire estuary, France. Following the OSMAC approach with the use of 14 culture media, the effect of salinity and of a mussel-derived medium on the metabolic expression were analysed using HPLC-UV/DAD-HRMS/MS. An untargeted metabolomics study was performed using principal component analysis (PCA), orthogonal projection to latent structure discriminant analysis (O-PLSDA) and molecular networking (MN). It highlighted some compounds belonging to sterols, macrolides and pyran-2-ones, which were specifically induced in marine conditions. In particular, a high chemical diversity of pyran-2-ones was found to be related to the presence of mussel extract in the culture medium. Mass spectrometry (MS)- and UV-guided purification resulted in the isolation of five new natural fungal pyran-2-one derivatives—5,6-dihydro-6S-hydroxymethyl-4-methoxy-2H-pyran-2-one (1), (6S, 1’R, 2’S)-LL-P880β (3), 5,6-dihydro-4-methoxy-6S-(1’S, 2’S-dihydroxy pent-3’(E)-enyl)-2H-pyran-2-one (4), 4-methoxy-6-(1’R, 2’S-dihydroxy pent-3’(E)-enyl)-2H-pyran-2-one (6) and 4-methoxy-2H-pyran-2-one (7)—together with the known (6S, 1’S, 2’S)-LL-P880β (2), (1’R, 2’S)-LL-P880γ (5), 5,6-dihydro-4-methoxy-2H-pyran-2-one (8), (6S, 1’S, 2’R)-LL-P880β (9), (6S, 1’S)-pestalotin (10), 1’R-dehydropestalotin (11) and 6-pentyl-4-methoxy-2H-pyran-2-one (12) from the mussel-derived culture medium extract. The structures of 1-12 were determined by 1D- and 2D-MMR experiments as well as high-resolution tandem MS, ECD and DP4 calculations. Some of these compounds were evaluated for their cytotoxic, antibacterial, antileishmanial and in-silico PTP1B inhibitory activities. These results illustrate the utility in using host-derived media for the discovery of new natural products.
From Sea to Skin: Is There a Future for Natural Photoprotectants?
Authors: Milito Alfonsina , Castellano Immacolata , Damiani Elisabetta
Published: June 2021

In the last few decades, the thinning of the ozone layer due to increased atmospheric pollution has exacerbated the negative effects of excessive exposure to solar ultraviolet radiation (UVR), and skin cancer has become a major public health concern. In order to prevent skin damage, public health advice mainly focuses on the use of sunscreens, along with wearing protective clothing and avoiding sun exposure during peak hours. Sunscreens present on the market are topical formulations that contain a number of different synthetic, organic, and inorganic UVR filters with different absorbance profiles, which, when combined, provide broad UVR spectrum protection. However, increased evidence suggests that some of these compounds cause subtle damage to marine ecosystems. One alternative may be the use of natural products that are produced in a wide range of marine species and are mainly thought to act as a defense against UVR-mediated damage. However, their potential for human photoprotection is largely under-investigated. In this review, attention has been placed on the molecular strategies adopted by marine organisms to counteract UVR-induced negative effects and we provide a broad portrayal of the recent literature concerning marine-derived natural products having potential as natural sunscreens/photoprotectants for human skin. Their chemical structure, UVR absorption properties, and their pleiotropic role as bioactive molecules are discussed. Most studies strongly suggest that these natural products could be promising for use in biocompatible sunscreens and may represent an alternative eco-friendly approach to protect humans against UV-induced skin damage.
The Evolution of the Use of Extracorporeal Membrane Oxygenation in Respiratory Failure
Authors: Feldhaus Danielle , Brodie Daniel , Lemaitre Philippe , Sonett Joshua , Agerstrand Cara
Published: June 2021

Extracorporeal membrane oxygenation (ECMO) has been used with increasing frequency to support patients with acute respiratory failure, most commonly, and severe forms of acute respiratory distress syndrome (ARDS). The marked increase in the global use of ECMO followed the publication of a large randomized trial in 2009 and the experience garnered during the 2009 influenza A (H1N1) pandemic, and has been further supported by the release of a large, randomized clinical trial in 2018, confirming a benefit from using ECMO in patients with severe ARDS. Despite a rapid expansion of ECMO-related publications, optimal management of patients receiving ECMO, in terms of patient selection, ventilator management, anticoagulation, and transfusion strategies, is evolving. Most recently, ECMO is being utilized for an expanding variety of conditions, including for cases of severe pulmonary or cardiac failure from coronavirus disease 2019 (COVID-19). This review evaluates modern evidence for ECMO for respiratory failure and the current challenges in the field.
Research on Parameters of Wire-Filling Laser Welding and Quenching Process for Joints Microstructure and Mechanical Property of BR1500HS Steel
Authors: Zhou Lianpu , Zhu Chundong , Ma Rongfei , Wei Zihao
Published: June 2021

With the aim to investigate the effect of parameters and the quenching process on the joint microstructure and mechanical properties of hot stamping steel by laser welding, BR1500HS boron steel was welded by wire-filling laser welding with ER70-G welding wire under different parameters. The welded specimens were heated to 900 °C and held for 5 min before water quenching. A universal material test machine, optical microscope, Vickers hardness tester, scanning electron microscope, and electron backscatter diffraction (EBSD) were used to characterize. The results show that the heat input should be greater than 1040 J/cm and the optimal wire-feeding speed is between 160 cm/min and 180 cm/min. The tensile strength of the quenched joint can reach greater than 1601.9 MPa at compatible parameters. More retained austenite distributes in the fusion zone (FZ) and fine grain zone (FGZ) than the coarse grain zone (CGZ) before quenching. However, the retained austenite in FZ and heat-affected zone (HAZ) decreases clearly and distributes uniformly after quenching. The grain diameter in FZ before quenching is not uniform and there are some coarse grains with the diameter greater than 40 μm. After quenching, the grains are refined and grain diameter is more uniform in the joint. With the increase in heat input, the microhardness of FZ and HAZ before quenching decreases from 500 HV to 450 HV. However, if the wire-feeding speed increases, the microhardness of FZ and HAZ before quenching increases from 450 HV to 500 HV. After quenching, the joint microhardness of all samples is between 450 HV and 550 HV. The fracture morphology of the joint before quenching consists of a large number of dimples and little river patterns. After quenching, the fracture morphology consists of a large amount of river patterns and cleavage facets due to the generation of martensite.
Fabrication of Ultranarrow Nanochannels with Ultrasmall Nanocomponents in Glass Substrates
Authors: Kamai Hiroki , Xu Yan
Published: June 2021

Nanofluidics is supposed to take advantage of a variety of new physical phenomena and unusual effects at nanoscales typically below 100 nm. However, the current chip-based nanofluidic applications are mostly based on the use of nanochannels with linewidths above 100 nm, due to the restricted ability of the efficient fabrication of nanochannels with narrow linewidths in glass substrates. In this study, we established the fabrication of nanofluidic structures in glass substrates with narrow linewidths of several tens of nanometers by optimizing a nanofabrication process composed of electron-beam lithography and plasma dry etching. Using the optimized process, we achieved the efficient fabrication of fine glass nanochannels with sub-40 nm linewidths, uniform lateral features, and smooth morphologies, in an accurate and precise way. Furthermore, the use of the process allowed the integration of similar or dissimilar material-based ultrasmall nanocomponents in the ultranarrow nanochannels, including arrays of pockets with volumes as less as 42 zeptoliters (zL, 10−21 L) and well-defined gold nanogaps as narrow as 19 nm. We believe that the established nanofabrication process will be very useful for expanding fundamental research and in further improving the applications of nanofluidic devices.
From Nanoparticles to Cancer Nanomedicine: Old Problems with New Solutions
Authors: Chiang Chi-Ling , Cheng Ming-Huei , Lin Chih-Hsin
Published: June 2021

Anticancer nanomedicines have been studied over 30 years, but fewer than 10 formulations have been approved for clinical therapy today. Despite abundant options of anticancer drugs, it remains challenging to have agents specifically target cancer cells while reducing collateral toxicity to healthy tissue. Nanocompartments that can be selective toward points deeply within malignant tissues are a promising concept, but the heterogeneity of tumor tissue, inefficiency of cargo loading and releasing, and low uniformity of manufacture required from preclinical to commercialization are major obstacles. Technological advances have been made in this field, creating engineered nanomaterials with improved uniformity, flexibility of cargo loading, diversity of surface modification, and less inducible immune responses. This review highlights the developmental process of approved nanomedicines and the opportunities for novel materials that combine insights of tumors and nanotechnology to develop a more effective nanomedicine for cancer patients.
The Role of miR-155 in Nutrition: Modulating Cancer-Associated Inflammation
Authors: Zanoaga Oana , Braicu Cornelia , Chiroi Paul , Andreea Nutu , Hajjar Nadim Al , Mărgărit Simona , Korban Schuyler S. , Berindan-Neagoe Ioana
Published: June 2021

Nutrition plays an important role in overall human health. Although there is no direct evidence supporting the direct involvement of nutrition in curing disease, for some diseases, good nutrition contributes to disease prevention and our overall well-being, including energy level, optimum internal function, and strength of the immune system. Lately, other major, but more silent players are reported to participate in the body’s response to ingested nutrients, as they are involved in different physiological and pathological processes. Furthermore, the genetic profile of an individual is highly critical in regulating these processes and their interactions. In particular, miR-155, a non-coding microRNA, is reported to be highly correlated with such nutritional processes. In fact, miR-155 is involved in the orchestration of various biological processes such as cellular signaling, immune regulation, metabolism, nutritional responses, inflammation, and carcinogenesis. Thus, this review aims to highlight those critical aspects of the influence of dietary components on gene expression, primarily on miR-155 and its role in modulating cancer-associated processes.
Diet Composition, Glucose Homeostasis, and Weight Regain in the YoYo Study
Authors: van Baak Marleen A. , Roumans Nadia J. T. , Mariman Edwin C. M.
Published: June 2021

Based on several randomized clinical trials, it has been suggested that baseline glucose homeostasis interacts with the influence of diet composition on weight loss and weight loss maintenance. In this secondary analysis of the YoYo study, a study investigating predictors of weight loss maintenance, we tested the hypothesis that (self-selected) dietary carbohydrate and/or fibre intake interact with the glucose homeostasis parameters for weight loss maintenance. Sixty-one overweight or obese individuals lost around 10 kg of body weight on an energy-restricted diet and were then followed for 9 months. During this period, participants were advised to maintain their body weight and eat a healthy diet without further recommendations on calorie intake or diet composition. Contrary to our hypothesis, carbohydrate intake showed no positive association with weight regain after weight loss, and no interaction with baseline fasting glucose concentration was found. There was a non-significant negative association between fibre intake and weight regain (B = −0.274, standard error (SE) 0.158, p = 0.090), but again, no interaction with fasting plasma glucose was found. In conclusion, the data from the YoYo study do not support a role for baseline glucose homeostasis in determining the association between self-reported carbohydrate and/or fibre intake and weight regain after weight loss.
Simultaneous Pretreatment of Aspirin and Omega-3 Fatty Acid Attenuates Nuclear Factor-κB Activation in a Murine Model with Ventilator-Induced Lung Injury
Authors: Kwack Won-Gun , Lee Yoon-Je , Eo Eun-Young , Chung Jin-Haeng , Lee Jae-Ho , Cho Young-Jae
Published: June 2021

Ventilator-induced lung injury (VILI) is an important critical care complication. Nuclear factor-κB (NF-κB) activation, a critical signaling event in the inflammatory response, has been implicated in the tracking of the lung injury. The present study aimed to determine the effect of simultaneous pretreatment with enteral aspirin and omega-3 fatty acid on lung injury in a murine VILI model. We compared the lung inflammation after the sequential administration of lipopolysaccharides and mechanical ventilation between the pretreated simultaneous enteral aspirin and omega-3 fatty acid group and the non-pretreatment group, by quantifying NF-κB activation using an in vivo imaging system to detect bioluminescence signals. The pretreated group with enteral aspirin and omega-3 fatty acid exhibited a smaller elevation of bioluminescence signals than the non-pretreated group (p = 0.039). Compared to the non-pretreated group, the pretreatment group with simultaneous enteral aspirin and omega-3 fatty acid showed reduced expression of the pro-inflammatory cytokine, tumor necrosis factor-α, in bronchoalveolar lavage fluid (p = 0.038). Histopathological lung injury scores were also lower in the pretreatment groups compared to the only injury group. Simultaneous pretreatment with enteral administration of aspirin and omega-3 fatty acid could be a prevention method for VILI in patients with impending mechanical ventilation therapy.
The Role of BMI, Body Fat Mass and Visceral Fat in Executive Function in Individuals with Overweight and Obesity
Authors: Sánchez-SanSegundo Miriam , Zaragoza-Martí Ana , Martin-LLaguno Iciar , Berbegal Marina , Ferrer-Cascales Rosario , Hurtado-Sánchez José Antonio
Published: June 2021

Evidence accumulated to date suggests that excess weight in the adult population is associated with a wide range of impairments in executive function. However, most studies have only examined the influence of body mass index (BMI) on the cognitive function of individuals with overweight and obesity. This study examined the potential associations of markers of adiposity (BMI, body fat, and visceral fat) with five domains of executive function including cognitive flexibility, inhibition, monitoring, planning, and working memory in a sample of 87 adult with overweight (n = 34) and obesity (n = 53). The results show that obese people had poorer working memory than those with overweight. After controlling for educational levels and physical activity, the results suggest that neither the waist–hip index not visceral fat were associated with cognitive function. In overweight, body fat was negatively associated with executive components of inhibition (p = 0.05) and monitoring (p = 0.02). In the obesity subgroup, body fat was negatively associated with inhibition (0.02) and working memory (0.04). The results provide evidence of the importance of adiposity for cognitive function. The implications for understanding the influence of markers of adiposity in adults with overweight and obesity are discussed.
Association between Chronotype and Nutritional, Clinical and Sociobehavioral Characteristics of Adults Assisted by a Public Health Care System in Brazil
Authors: Reis-Canaan Juliana C. , Canaan Marcelo M. , Costa Patrícia D. , Rodrigues-Juliatte Tamires P. , Pereira Michel C. A. , Castelo Paula M. , Pardi Vanessa , M. Murata Ramiro , Pereira Luciano J.
Published: June 2021

Chronotype (CT) has been associated with predisposition to chronic noncommunicable diseases (CNCDs), such as diabetes mellitus and obesity. However, the effects of CT on individuals assisted by public health systems (PHSs) in middle-up economies are still poorly explored. The objective of this study was to evaluate the relationship between CT and clinical, sociobehavioral and nutritional aspects in adults assisted by a PHS in Brazil. This is a population-based cross-sectional study. The sample consisted of 380 individuals, selected through probabilistic sampling by clusters, in all health units in a city of approximately 100 thousand inhabitants. Data collection was performed during home visits, by means of general and nutritional interviews, anthropometric measurements and the Morningness–Eveningness Questionnaire (MEQ). Statistical analysis comprised chi-square test and principal component analysis (CPA) followed by Fisher’s discriminant analysis to determine aspects associated with each CT (morning, evening or intermediate). With the aim of explaining the variation in the CT scores, the consumption of micronutrients (corrected to the total energy intake) and other individual and sociodemographic variables were used as explanatory factors in the adjustment of a linear regression model. The morning group was characterized by older men, with less than eight years of schooling, with low body mass index (BMI) and with low intake of omega-6, omega-3, sodium, zinc, thiamine, pyridoxine and niacin. The evening group, on the other hand, was composed of younger individuals, with a high consumption of these same nutrients, with high BMI and a higher frequency of heart diseases (p < 0.05). It was concluded that most morning CT individuals were elderly thin males with lower consumption of omega-6 and -3, sodium, zinc, thiamine, pyridoxine and niacin, whereas evening individuals were younger, had higher BMI and had higher consumption of the studied micronutrients. The identification of circadian and behavioral risk groups can help to provide preventive and multidisciplinary health promotion measures.
The Feasibility and Tolerability of Medium Chain Triglycerides in Women with a Catamenial Seizure Pattern on the Modified Atkins Diet
Authors: Felton Elizabeth A. , Henry-Barron Bobbie J. , Jan Amanda K. , Shegelman Abigail , Faltersack Kelly , Vizthum Diane , Cervenka Mackenzie C.
Published: June 2021

Ketogenic diet therapy (KDT), particularly modified Atkins diet (MAD), is increasingly recognized as a treatment for adults with epilepsy. Women with epilepsy (WWE) comprise 50% of people with epilepsy and approximately one in three have catamenial epilepsy. The purpose of this study was to determine whether adding a medium chain triglyceride emulsion to MAD to target catamenial seizures was feasible and well-tolerated. This was a prospective two-center study of pre-menopausal WWE with a catamenial seizure pattern on MAD. After a 1-month baseline interval with no changes in treatment, participants consumed betaquik® (Vitaflo International Ltd.) for 10 days each menstrual cycle starting 2 days prior to and encompassing the primary catamenial seizure pattern for five cycles. Participants recorded seizures, ketones, and menses, and completed surveys measuring tolerability. Sixteen women aged 20–50 years (mean 32) were enrolled and 13 (81.2%) completed the study. There was 100% adherence for consuming betaquik® in the women who completed the study and overall intervention adherence rate including the participants that dropped out was 81.2%. The most common side effects attributed to MAD alone prior to starting betaquik® were constipation and nausea, whereas abdominal pain, diarrhea, and nausea were reported after adding betaquik®. The high adherence rate and acceptable tolerability of betaquik® shows feasibility for future studies evaluating KDT-based treatments for catamenial seizures.
Fruit Pouch Consumption and Dietary Patterns Related to BMIz at 18 Months of Age
Authors: Lundkvist Ellen , Stoltz Sjöström Elisabeth , Lundberg Richard , Silfverdal Sven-Arne , West Christina E. , Domellöf Magnus
Published: June 2021

Concerns have been raised that an overconsumption of baby food fruit pouches among toddlers might increase the risk of childhood obesity. This study aimed to quantify the consumption of fruit pouches and other fruit containing food products and to explore potential correlations between the consumption of these products and body-mass index z-score (BMIz) at 18 months, taking other predictive factors into consideration. The study was based on 1499 children and one-month-recall food frequency questionnaires from the Swedish population-based birth cohort NorthPop. Anthropometric outcome data were retrieved from child health care records. BMIz at 18 months of age was correlated to maternal BMI and gestational weight gain and inversely correlated to fruit juice consumption and breastfeeding. BMIz at 18 months of age was not correlated to consumption of fruit pouches, sugar-sweetened beverages, whole fruit or milk cereal drink. Overweight at 18 months of age was correlated to maternal BMI and inversely correlated to breastfeeding duration. To our knowledge, this is the first study that investigates possible associations between baby food fruit pouch consumption and overweight in toddlers. We found that moderate fruit pouch consumption is not associated with excess weight at 18 months of age.
Effects of 6 Months of Soy-Enriched High Protein Compared to Eucaloric Low Protein Snack Replacement on Appetite, Dietary Intake, and Body Composition in Normal-Weight Obese Women: A Randomized Controlled Trial
Authors: Haghighat Neda , Ashtary-Larky Damoon , Bagheri Reza , Wong Alexei , Cheraghloo Neda , Moradpour Gholamreza , Nordvall Michael , Asbaghi Omid , Moeinvaziri Nader , Amini Masoud , Sohrabi Zahra , Dutheil Frédéric
Published: June 2021

(1) Background: The favorable effects of high protein snacks on body composition and appetite status in lean and athletic populations have been illustrated previously. However, the effects of soy-enriched high protein snacks have not been investigated in women with normal-weight obesity (NWO). Consequently, we aimed at comparing the effects of six months of soy-enriched high protein snack replacement on appetite, body composition, and dietary intake in women with NWO. (2) Methods: One hundred seven (107) women with NWO [(age: 24 ± 3 yrs, BMI: 22.7 ± 2.3 kg/m2, body fat percentage (BFP): 38 ± 3.2%)] who were assigned to one of two groups; high protein snack (HP, n = 52) containing 50 g soybean or isocaloric low-protein snack (protein: 18.2 g, carbohydrate: 15 g, fat: 10 g, energy: 210 kcal) or isocaloric low protein snack (LP, n = 55) containing 3.5 servings of fruit (protein: <2 g, carbohydrate: ≈50 g, fat: <1 g, energy: ≈210 kcal) as part of their daily meals (as a snack at 10 a.m.), successfully completed the study interventions. Body mass (BM), body mass index (BMI), waist circumference (WC), BFP, skeletal muscle mass, dietary intake, and appetite levels were evaluated prior to and after the six-month intervention. (3) Results: Appetite (HP = −12 mm and LP = −0.6 mm), energy intake (HP = −166.2 kcal/day and LP = 91.3 kcal), carbohydrate intake (HP = −58.4 g/day and LP = 6.4 g/day), WC (HP = −4.3 cm and LP = −0.9 cm), and BFP (HP = −3.7% and LP = −0.9%) were significantly (p < 0.05) reduced, while skeletal muscle mass (HP = 1.2 kg and LP = 0.3 kg) significantly increased in the HP compared to the LP group, respectively. (4) Conclusions: Six months of a soy-enriched high protein snack replacement decreased appetite and improved body composition in women with NWO. Our findings suggest that soy-enriched high protein snacks are an efficacious strategy for body composition improvement.
Activation of PI3K/Akt Signaling Pathway in Rat Hypothalamus Induced by an Acute Oral Administration of D-Pinitol
Authors: Medina-Vera Dina , Navarro Juan Antonio , Tovar Rubén , Rosell-Valle Cristina , Gutiérrez-Adan Alfonso , Ledesma Juan Carlos , Sanjuan Carlos , Pavón Francisco Javier , Baixeras Elena , Rodríguez de Fonseca Fernando , Decara Juan
Published: June 2021

D-Pinitol (DPIN) is a natural occurring inositol capable of activating the insulin pathway in peripheral tissues, whereas this has not been thoroughly studied in the central nervous system. The present study assessed the potential regulatory effects of DPIN on the hypothalamic insulin signaling pathway. To this end we investigated the Phosphatidylinositol-3-kinase (PI3K)/Protein Kinase B (Akt) signaling cascade in a rat model following oral administration of DPIN. The PI3K/Akt-associated proteins were quantified by Western blot in terms of phosphorylation and total expression. Results indicate that the acute administration of DPIN induced time-dependent phosphorylation of PI3K/Akt and its related substrates within the hypothalamus, indicating an activation of the insulin signaling pathway. This profile is consistent with DPIN as an insulin sensitizer since we also found a decrease in the circulating concentration of this hormone. Overall, the present study shows the pharmacological action of DPIN in the hypothalamus through the PI3K/Akt pathway when giving in fasted animals. These findings suggest that DPIN might be a candidate to treat brain insulin-resistance associated disorders by activating insulin response beyond the insulin receptor.
Oral Ferric Maltol Does Not Adversely Affect the Intestinal Microbiome of Patients or Mice, but Ferrous Sulphate Does
Authors: Mahalhal Awad , Frau Alessandra , Burkitt Michael D. , Ijaz Umer Z. , Lamb Christopher A. , Mansfield John C. , Lewis Stephen , Pritchard D. Mark , Probert Chris S.
Published: June 2021

Background and Aims: Altering dietary ferrous sulphate (FS) consumption exacerbates a murine model of colitis and alters the intestinal microbiome. We investigated the impact of oral ferric maltol (FM) and FS on mice with dextran sodium sulphate (DSS) induced colitis, and the microbiome of patients with iron deficiency. Methods: Mice had acute colitis induced, with 2% DSS for 5 days, followed by water. During this period, groups of mice were fed standard chow (200 ppm iron, SC, n = 8), or SC with 200ppm FS supplementation (n = 16, FSS), or SC with 200 ppm FM supplementation (n = 16, FMS). Clinical, pathological and microbiome assessments were compared at days 1 and 10. Fecal bacterial gDNA was extracted and the microbiome assessed by sequencing. Statistical inferences were made using MacQIIME. Principal Coordinates Analysis were used to visualize beta-diversity cluster analysis. Ten patients with IDA were treated with FS, and six with inactive inflammatory bowel disease received FM, supplements for four weeks: pre- and mid-treatment fecal samples were collected: the microbiome was assessed (see above). Results: In mice, after DSS treatment, there was a decrease in many genera in the SC and FSS groups: Lactobacillales increased in mice that received FMS. In humans, FS treatment led to an increase in five genera, but FM was not associated with any measurable change. The severity of DSS-induced colitis was greater with FSS than FMS. Conclusions: This study demonstrates differential and unique influences of ferric maltol and ferrous sulphate supplements on intestinal microbiota. These differences might contribute to the different side effects associated with these preparations.
β-Carotene Status Is Associated with Inflammation and Two Components of Metabolic Syndrome in Patients with and without Osteoarthritis
Authors: Yen Chi-Hua , Chang Po-Sheng , Chiu Ching-Ju , Huang Yu-Yun , Lin Ping-Ting
Published: June 2021

This study was conducted to investigate the β-carotene status in osteoarthritis (OA) patients and examine its relationships with the risk of inflammation and metabolic syndrome. OA patients were stratified by obesity based on body fat percentage (obese OA, n = 44; non-obese OA, n = 56), and sixty-nine subjects without OA or obesity were assigned as a non-obese control group. β-carotene, metabolic parameters, and inflammation status were assessed. Obese OA patients exhibited a significantly higher rate of metabolic syndrome (p = 0.02), abdominal obesity (p < 0.01), and lower β-carotene status (p < 0.01) compared with non-obese OA and non-obese controls. After adjusting for potential confounders, β-carotene status (≥0.8 µM) was significantly inversely correlated with the risk of metabolic syndrome (odds ratio = 0.27, p < 0.01), abdominal obesity (odds ratio = 0.33, p < 0.01), high blood pressure (odds ratio = 0.35, p < 0.01), hyperglycemia (odds ratio = 0.45, p < 0.05), and inflammation (odds ratio = 0.30, p = 0.01). Additionally, subjects who had a high β-carotene status with a low proportion of metabolic syndrome when they had a low-grade inflammatory status (p < 0.01). Obese OA patients suffered from a higher prevalence of metabolic syndrome and lower β-carotene status compared to the non-obese controls. A better β-carotene status (≥0.8 µM) was inversely associated with the risk of metabolic syndrome and inflammation, so we suggest that β-carotene status could be a predictor of the risk of metabolic syndrome and inflammation in patients with and without OA.
Remote Hyperspectral Imaging Acquisition and Characterization for Marine Litter Detection
Authors: Freitas Sara , Silva Hugo , Silva Eduardo
Published: June 2021

This paper addresses the development of a remote hyperspectral imaging system for detection and characterization of marine litter concentrations in an oceanic environment. The work performed in this paper is the following: (i) an in-situ characterization was conducted in an outdoor laboratory environment with the hyperspectral imaging system to obtain the spatial and spectral response of a batch of marine litter samples; (ii) a real dataset hyperspectral image acquisition was performed using manned and unmanned aerial platforms, of artificial targets composed of the material analyzed in the laboratory; (iii) comparison of the results (spatial and spectral response) obtained in laboratory conditions with the remote observation data acquired during the dataset flights; (iv) implementation of two different supervised machine learning methods, namely Random Forest (RF) and Support Vector Machines (SVM), for marine litter artificial target detection based on previous training. Obtained results show a marine litter automated detection capability with a 70–80% precision rate of detection in all three targets, compared to ground-truth pixels, as well as recall rates over 50%.
Multi-Scale Spatiotemporal Change Characteristics Analysis of High-Frequency Disturbance Forest Ecosystem Based on Improved Spatiotemporal Cube Model
Authors: Zhang Yangcen , Liu Xiangnan , Liu Meiling , Zou Xinyu , Zhang Qian , Peng Tao
Published: June 2021

High-frequency disturbance forest ecosystems undergo complex and frequent changes at various spatiotemporal scales owing to natural and anthropogenic factors. Effectively capturing the characteristics of these spatiotemporal changes from satellite image time series is a powerful and practical means for determining their causes and predicting their trends. Herein, we combined the spatiotemporal cube and vegetation indices to develop the improved spatiotemporal cube (IST-cube) model. We used this to acquire the spatiotemporal dynamics of forest ecosystems from 1987 to 2020 in the study area and then classified it into four spatiotemporal scales. The results showed that the cube-core only exists in the increasing IST-cubes, which are distributed in residential areas and forests. The length of the IST-cube implies the duration of triggers. Human activities result in long-term small-scope IST-cubes, and the impact in the vicinity of residential areas is increasing while there is no change within. Meteorological disasters cause short-term, large scope, and irregular impacts. Land use type change causes short-term small scope IST-cubes and a regular impact. Overall, we report the robustness and strength of the IST-cube model in capturing spatiotemporal changes in forest ecosystems, providing a novel method to examine complex changes in forest ecosystems via remote sensing.
Characterizing Off-Highway Road Use with Remote-Sensing, Social Media and Crowd-Sourced Data: An Application to Grizzly Bear (Ursus Arctos) Habitat
Authors: Kearney Sean P. , Larsen Terrence A. , Goodbody Tristan R. H. , Coops Nicholas C. , Stenhouse Gordon B.
Published: June 2021

Characterizing roads is important for conservation since the relationship between road use and ecological impact can vary across species. However, road use is challenging to monitor due to limited data and high spatial-temporal variability, especially for unpaved roads, which often coincide with critical habitats. In this study, we developed and evaluated two methods to characterize off-highway road use across a large management area of grizzly bear (Ursus arctos) habitat using: (1) a ‘network-based’ approach to connect human activity hotspots identified from social media posts and remotely detected disturbances and (2) an ‘image-based’ approach, in which we modeled road surface conditions and travel speed from high spatial resolution satellite imagery trained with crowd-sourced smartphone data. To assess the differences between these approaches and their utility for characterizing roads in the context of habitat integrity, we evaluated how behavioural patterns of global positioning system (GPS)-collared grizzly bears were related to road use characterized by these methods compared to (a) assuming all roads have equal human activity and (b) using a ‘reference’ road classification from a government database. The network- and image-based methods showed similar patterns of road use and grizzly bear response compared to the reference, and all three revealed nocturnal behaviour near high-use roads and better predicted grizzly bear habitat selection compared to assuming all roads had equal human activity. The network- and image-based methods show promise as cost-effective approaches to characterize road use for conservation applications where data is not available.
Impacts of Landscape Patterns on Ecosystem Services Value: A Multiscale Buffer Gradient Analysis Approach
Authors: Chen Wanxu , Zeng Jie , Chu Yumei , Liang Jiale
Published: June 2021

In recent decades, substantial changes have occurred in the spatial structure and form of landscapes in metropolises; these have greatly impacted ecosystem provision capacities. Clarifying the impact mechanism of landscape patterns on ecosystem services can provide insights into regional ecological conservation and sustainable development measures. Although previous studies have explored the impacts of landscape patterns on ecosystem services at multiple scales, few studies have been conducted using the buffer gradient analysis approach. Using land-use/cover change data, this study measured the evolution of spatiotemporal features of landscape patterns and ecosystem services value (ESV) with 1, 2, and 3 km buffer-zone scales in Wuhan, China. Econometric models were then used to analyze the impacts of landscape patterns on ecosystem services at different buffer-zone scales. The results demonstrated that rapid urbanization in Wuhan has led to significant changes in landscape patterns, and the landscape pattern metrics exhibited significant spatial heterogeneity. The ESV in Wuhan exhibited a steady decline during the study period. Hydrological regulations and waste treatment functions contributed to the largest proportion of ESV, and raw material production functions contributed to the lowest proportion. Landscape pattern metrics exerted a significant influence on ESV; however, this influence varied greatly. The results of this study provide a new understanding of the influence mechanism of landscape patterns on ecosystem services at 1, 2, and 3 km buffer-zone scales. These findings are critical for facilitating landscape planning and regional sustainable development.