bluuebunny/crossref_metadata_2025 huggingface dataset:
Visitor Forecasting Wisata Bahari Lamongan (WBL) Using Hybrid Particle Swarm Optimization (PSO) and
Seasonal ARIMA
Authors: Rahmalia Dinita
Published: December 2021

The revenue of city is determined by some factors, one of them is tourism sector. A problem of tourism
sector is forecasting visitors Wisata Bahari Lamongan (WBL). Because data of the number of visitors WBL are
fluctuating and seasonal, then it is required Seasonal ARIMA method. In the Seasonal ARIMA method, there are
some parameters that should be optimized for producing forecasting with small mean square error (MSE). In
this research, Seasonal ARIMA parameters will be optimized by Particle Swarm Optimization (PSO). PSO is
optimization algorithm inspired by behavior of birds group in searching food. Based on simulation results,
PSO algorithm can optimize Seasonal ARIMA parameter which is optimal and it can produce forecasting result
with small MSE.
Analysis and Development of KEBI 1.0 Checker Framework as an Application of Indonesian Spelling Error
Detection
Authors: Fahrudin Tresna Maulana , Sa'diyah Ilmatus , Latipah  , Atha Illah Ibnu Zahy , Beylirna Cagiva Chaedar , Acarya Burhan Syarif
Published: December 2021

At educational institutions, especially at University, writing scientific papers is a skill that must
be possessed by academics such as educators and students. However, writing scientific papers is not easy,
there are many provisions and rules that need to be fulfilled. Several studies show that there are still
many academics who make mistakes in writing their scientific papers. Some of the mistakes made include
punctuation errors, typographic writing errors and the use of non-standard words in Indonesian. Researchers
in Indonesia have developed various spelling error detection applications in Indonesian-language scientific
papers. This study tries to analyze the development of an application framework for detecting Indonesian
spelling errors from various assessment indicators. This study tries to compare the application framework
for detecting spelling errors between other studies with proposed application that named KEBI 1.0 Checker.
KEBI 1.0 Checker as a spelling error detection application has 3 main features, namely detecting errors in
the use of punctuation marks, writing typography, and using non-standard words in accordance with the
standards of the Big Indonesian Dictionary and the General Guidelines for Indonesian Spelling. In addition,
this study tries to objectively examine the complexity of the features, advantages and disadvantages,
methods and the level of accuracy of each application. The results of the analysis show that KEBI 1.0
Checker has the completeness of features, fast computation time, easy application access, and an attractive
user interface. However, it is still necessary to improve the precision in correcting spelling errors in
typographic words.
General concept on the time of exercise of the right to protection
Authors: Guyvan Petro
Published: December 2021

Purpose. The work is aimed at studying current issues on the temporal dimensions of subjective civil law and the possibilities of its protection in the event of an offense. After all, the issue of timely exercise of subjective law is very important in society. Therefore, the term is a necessary and integral element of the content of substantive civil law, the certainty in this regard will also provide certainty in the application of the necessary legal protection tools. Methods. From the methodological point of view, the work substantiates the thesis that with the expiration of the statute of limitations the regulatory right ceases and thus the subject of protection itself ceases. Results. This concept does not cover all cases of violation of subjective civil law, in particular, it is not able to justify the proper protection of property rights in cases where one of the rights of the owner is violated, such as the right of possession or use. Because the very fact of the offense in such cases does not terminate the right of ownership. Therefore, it is emphasized that the legal analysis of the commented temporal coordinates should be carried out not within the existence of the protected (regulatory) right, but within the protection, that is, what arose as a result of the offense. Scientific novelty. The modern development of civil doctrine allows us to conclude that the subjective substantive law is realized within the regulatory legal relationship, and in case of violation of the latter there is a new separate protective obligation, within which protection is carried out, in particular, through claims. At the same time, it is obvious that judicial protection does not exhaust the protective property of the law. In most cases, non-judicial remedies do not restrict the creditor's use of such remedies in time. Practical significance. The paper establishes that the right to exercise the protection authority, which is part of the content of the relevant protection obligation, exists for the duration of the right, except when the law explicitly establishes a special term of its validity. The violated substantive law after the expiration of the statute of limitations does not remain completely unprotected, although the degree of its protection is somewhat reduced.
Criminal and legal policy on the death penalty in Ukraine:
a historical and legal review of the XX-XXI centuries
Authors: Ostrohliad Oleksandr , Ilyn Liubomyr
Published: December 2021

Purpose. The purpose of the study is to reveal the historical background, legal consolidation of the existence and abolition of the type of death penalty in Ukraine. The research methodology is based on the principles of historicism, systematicity and objectivity. Methods. The interdisciplinary nature of the study led to the use of a range of general scientific, philosophical and special historical methods, among which, it is appropriate to distinguish the following: historical, comparative, document analysis and others.
Results. The scientific novelty of the study is due to the challenges facing Ukraine today, on the basis of which the need to restore the death penalty in Ukraine is being discussed. It is the historical and legal analysis of events and decisions of the XX-XXI centuries that allows us to look at this issue from a different angle, as a phenomenon not inherent in Ukraine as a European state. At the same time, it is expedient to determine the preconditions for the dominance in Ukrainian society of the belief in the effectiveness of the application of the maximum penalty. This is explained to us primarily by the low level of legal culture and stereotypes established in the Soviet period that the law is primarily a punishment. Scientific novelty. Conclusions. It is established that for the territory of the modern Ukrainian state, the death penalty was not an inherent phenomenon. All attempts to introduce this type of punishment are related to the fact that the territory of Ukraine is under the rule of other states. In the case of independence, this type of punishment was abolished as soon as possible, as Ukraine has always sought European standards of humanity and treatment of convicts, and the existence of the death penalty does not allow to achieve the goal of punishment and avoid miscarriage of justice. The main recommendations according to the results of the study are the formation of a holistic concept of humanization of criminal policy and spreading the understanding among the population that in itself, even the most severe type of punishment can not significantly affect the level and dynamics of crime.
Key dimensions of crises in the EU: contemporary aspects
Authors: Humeniuk Tetjana
Published: December 2021

Purpose. The aim of the article is to describe the main factors of crisis phenomena in the development of the European integration process. Methodology. The methodology involves a comprehensive study of theoretical and practical material on this subject, as well as formulation of relevant conclusions and recommendations. The following methods of scientific cognition were used in the research process: dialectical, terminological, formal and logical, comparative and legal, system and functional methods. Results. Transformation processes in the EU serve as a manifestation of global economic and information development. The crisis in the euro area was largely due to the peculiarities of its economic and institutional organization, in particular, the conditions for the free movement of capital and the lack of policy coordination in other sectors of the economy. Until now, measures in the field of economic governance in the EU have been largely ineffective due to their recommendatory nature and the lack of a legal obligation for EU Member States to comply with them. The crisis in the euro area and the EU in general has created favorable political conditions for deep institutional changes in the field of European integration. In particular, the dependence of EU countries on financial assistance from the ESM leads them to comply with the terms of the Fiscal Compact. At present, most EU political leaders consider deepening integration to be the key to securing the EU’s future economic growth and preventing future crises. In this context, the launch of a common fiscal policy is a significant step towards economic integration in the EU. Scientific novelty. The study found that most EU leaders are fully aware of the need for structural convergence, namely overcoming structural and cyclical differences among the economies of euro-area countries, as well as the main and secondary risks of divergence as a destructive phenomenon that can disrupt the established social, political, legal and economic order within the EU. Practical significance. Research materials can be used for comparative law studies.
Legal interpretative acts of the European court of human rights
Authors: Zavhorodnii V.
Published: December 2021

Purpose. The purpose of the article is to define and implement the characteristics of the decisions of the European Court of Human Rights, which have the legal properties of classical interpretative legal acts. Methodology. The theoretical tools of the study were: universal epistemological principles of cognition, complex, dialectical, axiological approaches, general scientific and special scientific methods of cognition. In particular, the following methods of scientific research were used during the research: analysis, synthesis, induction, deduction, structural, systemic, technical-dogmatic. Results. As a result of the study it was established that the legal acts of the European Court of Human Rights, which have the legal nature of interpretative-legal are: 1) advisory opinions of the Strasbourg Court, adopted by the European Court of Human Rights in connection with requests from the Committee of Ministers of the Council of Europe or the higher courts of the States Parties to the Convention; 2) judgments of the Strasbourg Court, containing explanations of legal positions and / or individual prescriptions previously formulated by the Court of Europe, contained in the decisions adopted by the Strasbourg Court on the merits. Scientific novelty. For the first time, the paper substantiates the legal nature of those legal acts of the Court of the Council of Europe that have exclusively interpretive properties. Practical significance. The results of the study can be used in law enforcement activities of entities authorized to implement the decisions of the European Court of Human Rights in the national legal order of Ukraine.
Effect of uchi-komi prescribed as high-intensity interval training on Judo athletes
Authors: Uchoa Fábio , De Souza Alves , De Freitas Victor Hugo
Published: December 2021

Objective: The aim of this study was to analyse the effects of a period of training performing uchi-komi prescribed as high-intensity interval training on the performance of judo athletes.

Method: Twenty judo athletes performed uchi-komi prescribed as high-intensity interval training (HIT; n = 10) or auto-oriented uchi-komi (control; n = 10). The judo athletes participated in three sessions (60 min for day) of Judo training per week, totalizing 18 sessions in six weeks. High-intensity interval training was performed with 30 s of entry to judo technique (all-out) and 15 s of rest, two times per week for six weeks. Athletes performed eight repetitions of high-intensity interval training in the first week, 10 repetitions in the second and third weeks, and 12 repetitions in the fourth, fifth, and sixth weeks. Pre and post training, athletes performed the Countermovement jump, Isometric judogi chin-up (isometric test), and Special judo fitness test.

Results: Special judo fitness test and isometric test showed interaction (p<0.01). Both the Control (p<0.01) and HIT groups (p<0.01) demonstrated improvement in the Special judo fitness test and isometric test. The Special judo fitness test presented a difference between groups at post (p<0.01) but not pre (p= 0.06). The isometric test presented a difference between groups at pre (p= 0.03) and post (p<0.01). Countermovement jump did not demonstrate interaction (p<0.77).

Conclusions: Training using uchi-komi prescribed as HIT improved performance in the Special judo fitness test and isometric test, without changing power in the lower limbs of judo athletes.
The effect of self myofascial release and static stretching on the antagonist muscles before agonist performance
Authors: Da Ressurreição Rogerson Pessanha , Pereira Eric Rosário , Martinez Luis Fernando , Nasser Igor , Souza João Antônio , Miranda Humberto
Published: December 2021

Objective: the aim of this study was to compare differences in volume load, total repetition performed and rating of perceived exertion between static stretching and self-myofascial release on antagonist muscles.

Methods: Eighteen recreationally trained men (23.4 ± 3.3 years; 80.7 ± 11.1 kg; 1.76 ± 0.06 cm) performed 10 repetitions maximum test and retest in the leg extension exercise on the first two visits. Then, three experimental sessions were conducted in a random order, in which two consisted of self-myofascial release and static stretching on hamstrings, and the other was used as a control.

Results: significant higher repetitions were performed in the third set of static stretching when compared to control protocol. Additionally, significant reductions in total repetitions performed were observed only in the control session. No significant differences were noticed in the volume load of leg extension and rating of perceived exertion between protocols.

Conclusion: self-myofascial release and static stretching performed before a session in the antagonist muscles can maintain repetitions performance by optimizing recovery between sets and reducing fatigue of agonist muscle.
Rendimiento de biomasa del pasto Saboya (Megathyrsus maximus) con relación a dos frecuencias de corte
Authors: Gómez Juan Carlos , Vascone Galarza Gustavo , Torres Pérez Jimmy , Moran Salazar Cristóbal Isaac
Published: December 2021

Introducción. El pasto Saboya (Megathyrsus maximus) es una de las especies de gran importancia en el sector ganadero por su gran producción de biomasa, además que en nuestro país es uno de los principales pastos con mayor superficie 1'147.091 hectáreas. El   ensayo se realizó en los predios de la Facultad de Ciencias Agropecuarias de la Universidad Técnica de Babahoyo, ubicada en el km. 7.5 de la vía Babahoyo-Montalvo. Objetivo. Determinar la producción de masa forrajera en fresco, materia seca y valor nutritivo en dos frecuencias de corte del pasto Saboya (Megathyrsus maximus). Metodología. Se utilizó el método experimental, donde se evaluaron las variables: rendimiento de materia fresca, porcentaje de materia seca y valor nutritivo en dos frecuencias de corte (40 y 60 días), se utilizó estadística no paramétrica para estimar la media aritmética. Resultados. El rendimiento de materia fresca que obtuvo el mayor peso con 2,80 kg por metro cuadrado fue a los 60 días de corte, mientras que a los 40 días obtuvo 2,03 kg.; en el porcentaje de materia seca mayor mostró el tratamiento de corte a los 40 días con 21,45 %. A diferencia del tratamiento de corte a los 60 días con 18,76 %; El mejor valor nutritivo se obtuvo a los 60 días con un promedio de 1,7 % para nitrógeno y 11,025 % de proteína cruda. El P, K, Ca y Mg obtuvieron promedios de: 0,27; 3,05; 0,51 y 0,20 respetivamente. Conclusión. El mayor rendimiento de materia fresca con un peso de 2,80 kg/m2 fue el tratamiento de corte a los 60 días; entre mayor sea los días de corte del pasto menor será la cantidad de materia seca, lo que se pudo evidenciar en la investigación. En cuanto al valor nutritivo a los 40 días de corte del pasto se obtuvo mejor promedio tanto para nitrógeno, proteína cruda y calcio.
Comparative analysis of the strength of restoration polymers of light polymerization for dental restoration using the acoustic emission method: part one
Authors:   Lviv National Medical University named after Danylo Halytsky, Lviv, Ukraine, Kukhta V. , Makeev V. ,   Lviv National Medical University named after Danylo Halytsky, Lviv, Ukraine, Kyrmanov O. ,   Lviv National Medical University named after Danylo Halytsky, Lviv, Ukraine, Skalsky V. ,   Physico-Mechanical Institute named after G.V. Karpenko National Academy of Sciences of Ukraine, Lviv, Ukraine, Stankevich O. ,   Physico-Mechanical Institute named after G.V. Karpenko National Academy of Sciences of Ukraine, Lviv, Ukraine
Published: December 2021

Purpose. Comparative analysis of the strength of hybrid restoration composites of light polymerization during their local loading using the phenomenon of acoustic emission. The following restoration composites were studied: Latelux, Tetric N-Ceram, Charisma Classic. Results. It was determined that the nature of the destruction of all composites is the same: elastic-plastic at the initial stage of the load with a transition to brittle as its further growth. Three types of composite failure are observed: correct, incorrect and mixed. The first predominated during fracture under the action of local loading of the Latelux composite, for Tetric N-Ceram and Charisma Classic materials a mixed type of fracture is characteristic. Conclusions. Analysis of the parameters of the AE signals showed that the signals had the highest amplitude and energy when the Tetric N-Ceram composite was destroyed, and the lowest – Latelux. All dental composites are dominated by high-energy ductile-brittle and brittle fracture, which indicates the spread of micro- and macrocracks of various sizes in materials. Key words: polymer composites, strength, acoustic emission method.
Comparative evaluation of the effectiveness of dental implants in patients with atrophy of the jaw bones with short implants and longer implants
Authors:   Yerevan State Medical University named after M. Heratsi, Republic of Armenia, Hakobyan G. , Boyajyan A. ,   Yerevan State Medical University named after M. Heratsi, Republic of Armenia, Dzhilazhyan A. ,   Yerevan State Medical University named after M. Heratsi, Republic of Armenia, Gambaryan N. ,   Yerevan State Medical University named after M. Heratsi, Republic of Armenia, Seyranyan V. ,   Yerevan State Medical University named after M. Heratsi, Republic of Armenia, Gevorgyan S. ,   Yerevan State Medical University named after M. Heratsi, Republic of Armenia, Khachatryan A. ,   Yerevan State Medical University named after M. Heratsi, Republic of Armenia
Published: December 2021

The aim of this study is to compare the survival rate of dental implants with short implants (< 8 mm) and longer implants ( 8 mm) in patients with atrophy of the jaw bones. Materials and research methods. This study is based on the analysis of treatment outcomes in 107 patients, 59 of whom received short implants (< 8 mm) and 48 patients received implants ( 8 mm) using a combination of augmentation. The study determined the engraftment of 5–6 mm Bicon implants and compared it with that of other implant sizes: Results. The average loss of marginal bone after one year was 0.96±0.25 mm for short implants and 0.94±0.46 for long implants. The survival rate of short implants (6–8 mm) after 3 years was 97.8 %, the survival rate of long (10–13 mm) implants was 98.1 %. The patients were satisfied with the aesthetic and functional result of the treatment. The results showed that implantation using short ones is effective in improving the chewing ability of patients in patients with atrophy of the jaw bones. In difficult clinical conditions, with a pronounced bone tissue deficiency, the use of short implants is the most gentle surgical method for restoring the missing support of fixed prostheses. Key words: atrophy of the jaw bones, dental implantation, short implants.
Regenerative therapy for the treatment of periimplantitis
Authors:   Yerevan State Medical University named after M. Heratsi, Republic of Armenia, Hakobyan G. , Seyranyan A. ,   Yerevan State Medical University named after M. Heratsi, Republic of Armenia, Khachatryan A. ,   Yerevan State Medical University named after M. Heratsi, Republic of Armenia, Burnazyan S. ,   Yerevan State Medical University named after M. Heratsi, Republic of Armenia, Seyranyan V. ,   Yerevan State Medical University named after M. Heratsi, Republic of Armenia
Published: December 2021

The purpose of this study is to evaluate the outcome of regenerative therapy of peri-implantitis. The 32 patients involved in this study with inflammatorydestructive processes in the field of peri-implant tissues of osseointegrated implants. The diagnostic parameters used for assessing peri-implantitis include clinical indices, Probing Pocket Depth (PPD), Bleeding On Probing (BOP), periimplant radiography. 16 implants with peri-implant mucositis, 8 implants with early peri-implantitis and 5 implants with moderate peri-implantitis was treated only conservative treatments methods, 6 implants with early peri-implantitis, 7 implants with moderate peri-implantitis and 4 implants with severe periimplantitis was treated surgically. PPD and BOP data at the re-examination were retrospectively compared to baseline data. A statistical significant reduction in both PPD and BOP were seen at alltimepoints as compared with the baseline clinical measurements. Stable clinical measurements PPD and BOP were demonstrated after 1 year the initial treatment, remaining stable during the following three years. Surgical regenerative treatment combined with mechanical and chemical detoxification of the implants’ surface, magneto-laser therapy and regenerative therapy using an autologous bone, xenograft, hyaluronic acid and a restorablemembrane a reliable method for stopping and treatment peri-implantitis. Key words: Peri-Implantitis; Dental Implant; Regenerative Therapy; Magneto-Laser Therapy; Hyaluronic Acid.
Diversity of tropical cryptophytes
Authors: Gusev Evgeniy S. , Martynenko Nikita A. , Kulizin Pavel V. , Podunay Yulia A.
Published: December 2021

The paper presents the preliminary data on the diversity of cryptomonad in the tropics (Vietnam and India). Isolation of algae into culture (more than 200 strains) and their study using molecular methods made it possible to reveal the rich flora of the genus Cryptomonas, consisting of 51 species (statistically supported clades).
Application of the ITS secondary structure in the identification of Nostoc species
Authors: Maltsev Yevhen I. , Maltseva Svetlana Y. , Kulikovskiy Maxim S.
Published: December 2021

The study of the species composition of Nostocales cyanobacteria in samples taken from different steppe ecosystems allowed us to analyze for the first time the diversity of the genus Nostoc in the Ukrainian steppe using molecular genetic methods. In the analysis, special attention was paid to the study of the predicted secondary structure of the ITS Box-B and V3 loops. During the analysis, it was shown that a number of isolated Nostoc strains, in addition to morphological ones, have significant genetic differences, which will allow us to propose new species for science based on further research.
Hubungan Peran Keluarga dengan Kemampuan Motorik Kasar Anak Disabilitas Intelektual
Authors: Purnamasari Nahdiah
Published: December 2021

Abstrak

Penelitian ini bertujuan untuk mengetahui hubungan peran keluarga  degan kemampuan motorik kasar anak disabilitas intelektual. Penelitian ini menggunakan desain cross-sectional dengan jumlah responden tiga puluh delapan orang (n=38). Terdapat beberapa data primer yang dikumpulkan diantaranya peran keluarga, kemampuan motorik kasar, Pengambilan data tersebut menggunakan kuisioner Family Role Questionnaire (FRQ) dan Form Test Gross Motor Development 2 (TGMD-2). Berdasarkan hasil analisis uji hubungan dengan Spearman Rho test didapatkan nilai signifikansi (p) sebesar 0,000(p< 0.05) yang berarti adanya hubungan antara peran keluarga dengan kemampuan motorik kasar anak disabilitas intelektual, dengan nilai r =0.062 yang berarti adanya hubungan yang kuat antara peran keluarga dengan kemampuan motorik kasar anak disabilitas intelektual.

Kata kunci : motorik kasar, peran keluarga, disabilitas intelektual.

 

Abstract

This study aims to see the relationship between family and gross motor skills of children with intellectual disabilities. This study used a cross-sectional design with thirty-eight respondents (n = 38). Some of the primary data collected included the role of the family, gross motor skills, data collection using the Family Role Questionnaire (FRQ) questionnaire and the Gross Motor Development 2 Test Form (TGMD-2). Based on the results of the analysis of the relationship test with the Spearman Rho test, it was obtained a significance value (p) of 0.000 (p <0.05), which means that there is a relationship between family roles and gross motor skills of children with intellectual disabilities, with a value of r = 0.062, which means that there is a strong relationship. between the role of the family and gross motor skills of children with intellectual disabilities.

Key words: gross motor skills, family roles, intellectual disabilities.
Formation and improvement f organizational and economic bases of forestry production in state forestry in Ukraine and Podillia region
Authors: Yaremko O.
Published: December 2021

Some issues on the historical formation of Podillia region of modern Ukraine and the formation of forestry in it are covered. It practically begins and acquires social meaning in the second half of the twentieth century. This means the use of land provided to economic entities (forestries) for growing wood as their main forestry product. The same applies to the cultivation and sale of natural resources related to forestry, secondary forest materials, services of various contents, etc. It is highlighted that the Forest Code of Ukraine has cunningly replaced the object of labor and use of the natural resource «Earth» with forest resources. The issues are somewhat complicated by the use of tax indicators and forms of their analysis and publication without their adaptation to the UN-ECE / FAO Standard Statistical Classification of Land Use and without the necessary grouping by forest and environmental components. The Government of Ukraine approves the Resolution «On Approval of the Procedure for Division of Forests into Categories and Allocation of Specially Protected Forest Areas», which does not bring national forest legislation closer to it. In this way, the further growth of non-timber forest lands at least 3 times or more in comparison with the leading European countries was legitimized.
The necessity of improving the content of the current Forest Code of Ukraine, as well as finalization and updating of the Instruction on forest management in the state forestry of Ukraine is proved. The availability of statistical and reporting information on the state of forests of the State Forest Agency of Ukraine for state-owned enterprises of the past allows to update the relevant reporting indicators while adapting them to the norms of European forestry. The proposed measures are aimed at improving forest legislation, as well as the formation of measures in the practical activities of forest management and the work of managers using its materials in forestry.
Hypertension and Stroke Cardiovascular Control Evaluation by Analyzing Blood Pressure, Cerebral Blood Flow, Blood Vessel Resistance and Baroreflex
Authors: Yeh Shoou-Jeng , Lung Chi-Wen , Jan Yih-Kuen , Kuo Fang-Chuan , Liau Ben-Yi
Published: December 2021

Cardiovascular diseases have been the leading causes of mortality in Taiwan and the world at large for decades. The composition of cardiovascular and cerebrovascular systems is quite complicated. Therefore, it is difficult to detect or trace the related signs of cardiovascular and cerebrovascular diseases. The characteristics and changes in cardiopulmonary system disease can be used to track cardiovascular and cerebrovascular disease prevention and diagnosis. This can effectively reduce the occurrence of cardiovascular and cerebrovascular diseases. This study analyzes the variability in blood pressure, cerebral blood flow velocity and the interaction characteristics using linear and nonlinear approaches in stroke, hypertension and healthy groups to identify the differences in cardiovascular control in these groups. The results showed that the blood pressure and cerebral blood flow of stroke patients and hypertensive patients were significantly higher than those of healthy people (statistical differences (p < 0.05). The cerebrovascular resistance (CVR) shows that the CVR of hypertensive patients is higher than that of healthy people and stroke patients (p < 0.1), indicating that the cerebral vascular resistance of hypertensive patients is slightly higher. From the patient’s blood flow and vascular characteristics, it can be observed that the cardiovascular system is different from those in healthy people. Baroreflex sensitivity (BRS) decreased in stroke patients (p < 0.05). Chaotic analysis revealed that the blood pressure disturbance in hypertensive patients has a higher chaotic behavior change and the difference in initial state sensitivity. Cross-correlation (CCF) analysis shows that as the course of healthy→hypertension→stroke progresses, the maximum CCF value decreases significantly (p < 0.05). That means that blood pressure and cerebral blood flow are gradually not well controlled by the self-regulation mechanism. In conclusion, cardiovascular control performance in hypertensive and stroke patients displays greater variation. This can be observed by the bio-signal analysis. This analysis could identify a measure for detecting and preventing the risk for hypertension and stroke in clinical practice. This is a pilot study to analyze cardiovascular control variation in healthy, hypertensive and stroke groups.
Introduction of Modified BglBrick System in Lactococcus lactis for Straightforward Assembly of Multiple Gene Cassettes
Authors: Plavec Tina Vida , Ključevšek Tim , Berlec Aleš
Published: December 2021

Genetic modification of lactic acid bacteria is an evolving and highly relevant field of research that allows the engineered bacteria to be equipped with the desired functions through the controlled expression of the recombinant protein. Novel genetic engineering techniques offer the advantage of being faster, easier and more efficient in incorporating modifications to the original bacterial strain. Here, we have developed a modified BglBrick system, originally introduced in Escherichia coli and optimized it for the lactic acid bacterium Lactococcus lactis. Six different expression cassettes, encoding model proteins, were assembled in different order as parts of a modified BglBrick system in a novel plasmid pNBBX. All cassettes included nisin promoter, protein encoding gene and transcription terminator. We demonstrated successful intracellular expression of the two fluorescent proteins and display of the four protein binders on the bacterial surface. These were expressed either alone or concomitantly, in combinations of three model proteins. Thus, a modified BglBrick system developed herein enables simple and modular construction of multigene plasmids and controlled simultaneous expression of three proteins in L. lactis.
Developmental Disruption of Erbb4 in Pet1+ Neurons Impairs Serotonergic Sub-System Connectivity and Memory Formation
Authors: Barettino Candela , Ballesteros-Gonzalez Álvaro , Aylón Andrés , Soler-Sanchis Xavier , Ortí Leticia , Díaz Selene , Reillo Isabel , García-García Francisco , Iborra Francisco José , Lai Cary , Dehorter Nathalie , Leinekugel Xavier , Flames Nuria , Del Pino Isabel
Published: December 2021

The serotonergic system of mammals innervates virtually all the central nervous system and regulates a broad spectrum of behavioral and physiological functions. In mammals, serotonergic neurons located in the rostral raphe nuclei encompass diverse sub-systems characterized by specific circuitry and functional features. Substantial evidence suggest that functional diversity of serotonergic circuits has a molecular and connectivity basis. However, the landscape of intrinsic developmental mechanisms guiding the formation of serotonergic sub-systems is unclear. Here, we employed developmental disruption of gene expression specific to serotonergic subsets to probe the contribution of the tyrosine kinase receptor ErbB4 to serotonergic circuit formation and function. Through an in vivo loss-of-function approach, we found that ErbB4 expression occurring in a subset of serotonergic neurons, is necessary for axonal arborization of defined long-range projections to the forebrain but is dispensable for the innervation of other targets of the serotonergic system. We also found that Erbb4-deletion does not change the global excitability or the number of neurons with serotonin content in the dorsal raphe nuclei. In addition, ErbB4-deficiency in serotonergic neurons leads to specific behavioral deficits in memory processing that involve aversive or social components. Altogether, our work unveils a developmental mechanism intrinsically acting through ErbB4 in subsets of serotonergic neurons to orchestrate a precise long-range circuit and ultimately involved in the formation of emotional and social memories.
Isolation and Characterization of Highly Pure Type A Spermatogonia From Sterlet (Acipenser ruthenus) Using Flow-Cytometric Cell Sorting
Authors: Xie Xuan , Tichopád Tomáš , Kislik Galina , Langerová Lucie , Abaffy Pavel , Šindelka Radek , Franěk Roman , Fučíková Michaela , Steinbach Christoph , Shah Mujahid Ali , Šauman Ivo , Chen Fan , Pšenička Martin
Published: December 2021

Sturgeons are among the most ancient linages of actinopterygians. At present, many sturgeon species are critically endangered. Surrogate production could be used as an affordable and a time-efficient method for endangered sturgeons. Our study established a method for identifying and isolating type A spermatogonia from different developmental stages of testes using flow cytometric cell sorting (FCM). Flow cytometric analysis of a whole testicular cell suspension showed several well-distinguished cell populations formed according to different values of light scatter parameters. FCM of these different cell populations was performed directly on glass slides for further immunocytochemistry to identify germ cells. Results showed that the cell population in gate P1 on a flow cytometry plot (with high forward scatter and high side scatter parameter values) contains the highest amount of type A spermatogonia. The sorted cell populations were characterized by expression profiles of 10 germ cell specific genes. The result confirmed that setting up for the P1 gate could precisely sort type A spermatogonia in all tested testicular developmental stages. The P2 gate, which was with lower forward scatter and side scatter values mostly, contained type B spermatogonia at a later maturing stage. Moreover, expressions ofplzf, dnd,boule,andkitrwere significantly higher in type A spermatogonia than in later developed germ cells. In addition,plzfwas firstly found as a reliable marker to identify type A spermatogonia, which filled the gap of identification of spermatogonial stem cells in sterlet. It is expected to increase the efficiency of germ stem cell culture and transplantation withplzfidentification. Our study thus first addressed a phenotypic characterization of a pure type A spermatogonia population in sterlet. FCM strategy can improve the production of sturgeons with surrogate broodstock and further the analysis of the cellular and molecular mechanisms of sturgeon germ cell development.
Myeloid Immune Cells CARrying a New Weapon Against Cancer
Authors: Ramos Rodrigo Nalio , Couto Samuel Campanelli Freitas , Oliveira Theo Gremen M. , Klinger Paulo , Braga Tarcio Teodoro , Rego Eduardo Magalhães , Barbuto José Alexandre M. , Rocha Vanderson
Published: December 2021

Chimeric antigen receptor (CAR) engineering for T cells and natural killer cells (NK) are now under clinical evaluation for the treatment of hematologic cancers. Although encouraging clinical results have been reported for hematologic diseases, pre-clinical studies in solid tumors have failed to prove the same effectiveness. Thus, there is a growing interest of the scientific community to find other immune cell candidate to express CAR for the treatment of solid tumors and other diseases. Mononuclear phagocytes may be the most adapted group of cells with potential to overcome the dense barrier imposed by solid tumors. In addition, intrinsic features of these cells, such as migration, phagocytic capability, release of soluble factors and adaptive immunity activation, could be further explored along with gene therapy approaches. Here, we discuss the elements that constitute the tumor microenvironment, the features and advantages of these cell subtypes and the latest studies using CAR-myeloid immune cells in solid tumor models.
Modulation of Phase Separation by RNA: A Glimpse on N6-Methyladenosine Modification
Authors: Su Yingfeng , Maimaitiyiming Yasen , Wang Lingfang , Cheng Xiaodong , Hsu Chih-Hung
Published: December 2021

Phase separation is the driving force behind formation of various biomolecular condensates (BioMCs), which sub-compartmentalize certain cellular components in a membraneless manner to orchestrate numerous biological processes. Many BioMCs are composed of proteins and RNAs. While the features and functions of proteins are well studied, less attention was paid to the other essential component RNAs. Here, we describe how RNA contributes to the biogenesis, dissolution, and properties of BioMCs as a multivalence providing scaffold for proteins/RNA to undergo phase separation. Specifically, we focus on N6-methyladenosine (m6A), the most widely distributed dynamic post-transcriptional modification, which would change the charge, conformation, and RNA-binding protein (RBP) anchoring of modified RNA. m6A RNA-modulated phase separation is a new perspective to illustrate m6A-mediated various biological processes. We summarize m6A main functions as “beacon” to recruit reader proteins and “structural switcher” to alter RNA–protein and RNA–RNA interactions to modulate phase separation and regulate the related biological processes.
Wild Boar Events and the Veterinarization of Multispecies Coexistence
Authors: Broz Ludek , Arregui Aníbal Garcia , O'Mahony Kieran
Published: December 2021

By considering the emergence and threat of African Swine Fever (ASF) in Europe, this paper demonstrates the growing role of veterinary rationales in reframing contemporary human-wild boar coexistence. Through comparative ethnographies of human-wild boar relations in the Czech Republic, Spain and England, it shows that coexistence is not a predictable and steady process but is also demarked by points of radical change in form, course and atmosphere. Such moments, orwild boar events, can lead to the (re-)formation or magnified influence of certain discourses, practices and power relations in determining strategies of bio-governance. Specifically, this paper highlights how the spread of ASF in Europe has accelerated an already ongoing process ofveterinarization, understood as the growing prominence of veterinary sciences in the mediation and reorganization of contemporary socioecologies. This example highlights how veterinary logics increasingly influence localized human-wildlife relations and, through analogous practices of biosecurity and control, also connect different places and geographic contexts.
Surgical Management for a Rare Pedunculated Left Ventricular Apical Lipoma: A Case Report and Review of Literature
Authors: Song Long , Iroegbu Chukwuemeka Daniel , Yang Jinfu , Fan Chengming
Published: December 2021

Cardiac lipomas, though extremely rare, are encapsulated tumors composed primarily of mature fat cells. Despite their benign character, cardiac lipomas can cause life-threatening complications by rapid growth. Cardiac lipomas, which are frequently located in the left ventricle (LV) or right atrium, can originate either from the subendocardium, subpericardium, or the myocardium. They are usually asymptomatic and carry a good prognosis during long-term follow-up; however, published reports show that untreated cardiac lipomas may be fatal when they cause arrhythmic or obstructive symptoms. In addition, several surgical options have been reported to obtain an appropriate operative view following poor visualization, primarily when tumors are located in the LV. Herein, we present a case of a pedunculated LV apical lipoma in a symptomatic patient successfully managed by surgical resection. We also discuss diagnostic modalities in surgical planning and the choice of surgical approach.
Positive Association Between Serum Alkaline Phosphatase and First Stroke in Hypertensive Adults
Authors: Zhang Yuanyuan , Li Huan , Xie Di , Li Jianping , Zhang Yan , Wang Binyan , Liu Chengzhang , Song Yun , Wang Xiaobin , Huo Yong , Hou Fan Fan , Xu Xiping , Qin Xianhui
Published: December 2021

The relation of alkaline phosphatase (ALP) with stroke risk remains uncertain. We aimed to examine the association between serum ALP and the risk of first stroke, and explore the possible effect modifiers in the association, among adults with hypertension. A total of 19,747 participants with baseline ALP measurements and without liver disease at baseline from the China Stroke Primary Prevention Trial (CSPPT) were included. The primary outcome was a first stroke. Over a median follow-up of 4.5 years, there was a positive association between serum ALP levels and the risk of first stroke (per SD increment, adjusted HR, 1.10; 95%CI: 1.01, 1.20). When serum ALP was evaluated as quartiles, a significantly higher risk of first stroke was observed in those in quartile 2–4 (ALP ≥79 IU/L; adjusted HR, 1.38; 95% CI: 1.11, 1.71), compared with participants in quartile 1 (ALP <79 IU/L). Similar results were found for first ischemic or hemorrhagic stroke. Similar findings were also found in those with a normal range of baseline ALP levels (20–140 IU/L) (per SD increment, adjusted HR, 1.15; 95%CI: 1.05, 1.27). None of the variables, including sex, age, body mass index, smoking, alcohol drinking, blood pressure, total cholesterol, fasting glucose levels at baseline, and blood pressure levels during the treatment period, significantly modified the association. In summary, our study suggests that higher serum ALP levels, even in normal range, were significantly related to higher risk of first stroke among Chinese hypertensive adults.
Cardiac Amyloidosis: A Review of Current Imaging Techniques
Authors: Razvi Yousuf , Patel Rishi K. , Fontana Marianna , Gillmore Julian D.
Published: December 2021

Systemic amyloidosis is a rare, heterogenous group of diseases characterized by extracellular infiltration and deposition of amyloid fibrils. Cardiac amyloidosis (CA) occurs when these fibrils deposit within the myocardium. Untreated, this inevitably leads to progressive heart failure and fatality. Historically, treatment has remained supportive, however, there are now targeted disease-modifying therapeutics available to patients with CA. Advances in echocardiography, cardiac magnetic resonance (CMR) and repurposed bone scintigraphy have led to a surge in diagnoses of CA and diagnosis at an earlier stage of the disease natural history. CMR has inherent advantages in tissue characterization which has allowed us to better understand the pathological disease process behind CA. Combined with specialist assessment and repurposed bone scintigraphy, diagnosis of CA can be made without the need for invasive histology in a significant proportion of patients. With existing targeted therapeutics, and novel agents being developed, understanding these imaging modalities is crucial to achieving early diagnosis for patients with CA. This will allow for early treatment intervention, accurate monitoring of disease course over time, and thereby improve the length and quality of life of patients with a disease that historically had an extremely poor prognosis. In this review, we discuss key radiological features of CA, focusing on the two most common types; immunoglobulin light chain (AL) and transthyretin (ATTR) CA. We highlight recent advances in imaging techniques particularly in respect of their clinical application and utility in diagnosis of CA as well as for tracking disease change over time.
Mitral Valve Atlas for Artificial Intelligence Predictions of MitraClip Intervention Outcomes
Authors: Dabiri Yaghoub , Yao Jiang , Mahadevan Vaikom S. , Gruber Daniel , Arnaout Rima , Gentzsch Wolfgang , Guccione Julius M. , Kassab Ghassan S.
Published: December 2021

Severe mitral regurgitation (MR) is a cardiac disease that can lead to fatal consequences. MitraClip (MC) intervention is a percutaneous procedure whereby the mitral valve (MV) leaflets are connected along the edge using MCs. The outcomes of the MC intervention are not known in advance, i.e., the outcomes are quite variable. Artificial intelligence (AI) can be used to guide the cardiologist in selecting optimal MC scenarios. In this study, we describe an atlas of shapes as well as different scenarios for MC implantation for such an AI analysis. We generated the MV geometrical data from three different sources. First, the patients' 3-dimensional echo images were used. The pixel data from six key points were obtained from three views of the echo images. Using PyGem, an open-source morphing library in Python, these coordinates were used to create the geometry by morphing a template geometry. Second, the dimensions of the MV, from the literature were used to create data. Third, we used machine learning methods, principal component analysis, and generative adversarial networks to generate more shapes. We used the finite element (FE) software ABAQUS to simulate smoothed particle hydrodynamics in different scenarios for MC intervention. The MR and stresses in the leaflets were post-processed. Our physics-based FE models simulated the outcomes of MC intervention for different scenarios. The MR and stresses in the leaflets were computed by the FE models for a single clip at different locations as well as two and three clips. Results from FE simulations showed that the location and number of MCs affect subsequent residual MR, and that leaflet stresses do not follow a simple pattern. Furthermore, FE models need several hours to provide the results, and they are not applicable for clinical usage where the predicted outcomes of MC therapy are needed in real-time. In this study, we generated the required dataset for the AI models which can provide the results in a matter of seconds.