bluuebunny/crossref_metadata_2025 huggingface dataset:
Stieltjes Differential Inclusions with Periodic Boundary Conditions without Upper Semicontinuity
Authors: Marraffa Valeria , Satco Bianca
Published: December 2021

We are studying first order differential inclusions with periodic boundary conditions where the Stieltjes derivative with respect to a left-continuous non-decreasing function replaces the classical derivative. The involved set-valued mapping is not assumed to have compact and convex values, nor to be upper semicontinuous concerning the second argument everywhere, as in other related works. A condition involving the contingent derivative relative to the non-decreasing function (recently introduced and applied to initial value problems by R.L. Pouso, I.M. Marquez Albes, and J. Rodriguez-Lopez) is imposed on the set where the upper semicontinuity and the assumption to have compact convex values fail. Based on previously obtained results for periodic problems in the single-valued cases, the existence of solutions is proven. It is also pointed out that the solution set is compact in the uniform convergence topology. In particular, the existence results are obtained for periodic impulsive differential inclusions (with multivalued impulsive maps and finite or possibly countable impulsive moments) without upper semicontinuity assumptions on the right-hand side, and also the existence of solutions is derived for dynamic inclusions on time scales with periodic boundary conditions.
Introducing Tables to Second-Grade Elementary Students in an Algebraic Thinking Context
Authors: Torres María D. , Brizuela Bárbara M. , Cañadas María C. , Moreno Antonio
Published: December 2021

In this study, we adopted a functional perspective on algebra. Our focus was on tables and how they were first used by second-grade elementary school students (7- and 8-year-olds) when working with functions. This qualitative, exploratory, and descriptive study consisted of five classroom sessions and two semi-structured interviews, one group and one individual, two weeks apart, focused on tasks involving different functions and uses of tables, with and without labels for headings. In this study, we ask the following research questions: How do children organise values in tables with or without (a priori) labels for headings? What are the regularities (structures) identified by students? Our data revealed that students were able to organise the values of variables by listing them in columns and labelling the headings (i.e., identifying the variables involved). The ways in which children organised the data in tables enabled us to identify the structures they identified as regularities between the variables involved in the functions. More structures were correctly identified in the second interview compared to the first.
Extension of the Wittrick-Williams Algorithm for Free Vibration Analysis of Hybrid Dynamic Stiffness Models Connecting Line and Point Nodes
Authors: Liu Xiang , Qiu Shaoqi , Xie Suchao , Banerjee Jnan Ranjan
Published: December 2021

This paper extends the Wittrick-Williams (W-W) algorithm for hybrid dynamic stiffness (DS) models connecting any combinations of line and point nodes. The principal novelties lie in the development of both the DS formulation and the solution technique in a sufficiently systematic and general manner. The parent structure is considered to be in the form of two dimensional DS elements with line nodes, which can be connected to rigid/spring point supports/connections, rod/beam point supports/connections, and point connections to substructures. This is achieved by proposing a direct constrain method in a strong form which makes the modeling process straightforward. For the solution technique, the W-W algorithm is extended for all of the above hybrid DS models. No matrix inversion is needed in the proposed extension, making the algorithm numerically stable, especially for complex built-up structures. A mathematical proof is provided for the extended W-W algorithm. The proposed DS formulation and the extended W-W algorithm are validated by the FE results computed by ANSYS. This work significantly extends the application scope of the DS formulation and the W-W algorithm in a methodical and reliable manner, providing a powerful eigenvalue analysis tool for beam-plate built-up structures.
Quantile Regression Analysis between the After-School Exercise and the Academic Performance of Korean Middle School Students
Authors: Shin Kyulee , You Sukkyung
Published: December 2021

This study deepens our understanding of the prediction and structural relationship between a student’s academic performance and his/her regular after-school exercise by estimating models based upon the quantile regression and the instrumental variable quantile regression methods, respectively. Using data on Korean middle school students, we found that negative relationships were dominant for the prediction models, whereas the relationships were reversed for the structural models, affirming the theoretical and experimental hypotheses observed in prior literature. Furthermore, we also found that the low-performing students, in terms of the academic performance, had stronger associations between the two variables than the high-performing students, overall.
Sepsis in Burns—Lessons Learnt from Developments in the Management of Septic Shock
Authors: Boehm Dorothee , Menke Henrik
Published: December 2021

After surviving the acute phase of resuscitation, septic shock is the cause of death in the majority of burn patients. Therefore, the management of septic shock is a cornerstone in modern burn care. Whereas sepsis therapy in general has undergone remarkable developments in the past decade, the management of septic shock in burn patients still has a long way to go. Instead, the differences of burn patients with septic shock versus general patients have been emphasized and thus, burn patients were excluded in every sepsis study which are the basis for modern sepsis therapy. However, due to the lack of evidence in burn patients, the standards of procedure for general sepsis therapy have been adopted in burn care. This review identifies the differences of burn patients with sepsis versus other septic patients and summarizes the scientific basis for modern sepsis therapy in general ICU patients and burn patients. Consequently, the results in general sepsis research should be transferred to burn care, which means the implementation of effective screening, early resuscitation, and efficient antimicrobial treatment. Therefore, on the basis of past developments and in the light of the current update of the Surviving Sepsis Campaign guidelines, this review introduces the “Burn SOFA score” and the “3 H’s of burn sepsis” as a screening tool for early sepsis recognition in burn patients.
Desalination and Detoxification of Textile Wastewater by Novel Photocatalytic Electrolysis Membrane Reactor for Ecosafe Hydroponic Farming
Authors: Aydin Muhammed Iberia , Ozaktac Damla , Yuzer Burak , Doğu Mustafa , Inan Hatice , Okten Hatice Eser , Coskun Serdar , Selcuk Huseyin
Published: December 2021

In this study, a novel photoelectrocatalytic membrane (PECM) reactor was tested as an option for the desalination, disinfection, and detoxification of biologically treated textile wastewater (BTTWW), with the aim to reuse it in hydroponic farming. The anionic ion exchange (IEX) process was used before PECM treatment to remove toxic residual dyes. The toxicity evaluation for every effluent was carried out using the Vibrio fischeri, Microtox® test protocol. The disinfection effect of the PECM reactor was studied against E. coli. After PECM treatment, the 78.7% toxicity level of the BTTWW was reduced to 14.6%. However, photocatalytic desalination during treatment was found to be slow (2.5 mg L−1 min−1 at 1 V potential). The reactor demonstrated approximately 52% COD and 63% TOC removal efficiency. The effects of wastewater reuse on hydroponic production were comparatively investigated by following the growth of the lettuce plant. A detrimental effect was observed on the lettuce plant by the reuse of BTTWW, while no negative impact was reported using the PECM treated textile wastewater. In addition, all macro/micronutrient elements in the PECM treated textile wastewater were recovered by hydroponic farming, and the PECM treatment may be an eco-safe wastewater reuse method for crop irrigation.
Green H2 Production by Water Electrolysis Using Cation Exchange Membrane: Insights on Activation and Ohmic Polarization Phenomena
Authors: Esposito Elisa , Minotti Angelo , Fontananova Enrica , Longo Mariagiulia , Jansen Johannes , Figoli Alberto
Published: December 2021

Low-temperature electrolysis by using polymer electrolyte membranes (PEM) can play an important role in hydrogen energy transition. This work presents a study on the performance of a proton exchange membrane in the water electrolysis process at room temperature and atmospheric pressure. In the perspective of applications that need a device with small volume and low weight, a miniaturized electrolysis cell with a 36 cm2 active area of PEM over a total surface area of 76 cm2 of the device was used. H2 and O2 production rates, electrical power, energy efficiency, Faradaic efficiency and polarization curves were determined for all experiments. The effects of different parameters such as clamping pressure and materials of the electrodes on polarization phenomena were studied. The PEM used was a catalyst-coated membrane (Ir-Pt-Nafion™ 117 CCM). The maximum H2 production was about 0.02 g min−1 with a current density of 1.1 A cm−2 and a current power about 280 W. Clamping pressure and the type of electrode materials strongly influence the activation and ohmic polarization phenomena. High clamping pressure and electrodes in titanium compared to carbon electrodes improve the cell performance, and this results in lower ohmic and activation resistances.
Removal of Copper (II), Zinc (II), Cobalt (II), and Nickel (II) Ions by PIMs Doped 2-Alkylimidazoles
Authors: Radzyminska-Lenarcik Elzbieta , Maslowska Kamila , Urbaniak Wlodzimierz
Published: December 2021

Polymer inclusion membranes (PIMs) are an attractive approach to the separation of metals from an aqueous solution. This study is concerned with the use of 2-alkylimidazoles (alkyl = methyl, ethyl, propyl, butyl) as ion carriers in PIMs. It investigates the separation of copper (II), zinc (II), cobalt (II), and nickel (II) from aqueous solutions with the use of polymer inclusion membranes. PIMs are formed by casting a solution containing a carrier (extractant), a plasticizer (o-NPPE), and a base polymer such as cellulose triacetate (CTA) to form a thin, flexible, and stable film. The topics discussed include transport parameters, such as the type of carrier, initial fluxes, separation coefficients of copper in relation to other metals, as well as transport recovery of metal ions. The membrane was characterized using AFM and SEM to obtain information on its composition.
Effect of Ni Doping on the Embrittlement of Liquid Zinc at Σ5 Fe Austenite Grain Boundary
Authors: Ding Chengfa , Peng Wangjun , Ma Zheng , Zhao Yan , Teng Huaxiang , Wu Guangxin
Published: December 2021

In this study, first-principles computational tensile tests have been performed for the Σ5 symmetrically tilted grain boundaries of the face-centered cubic (fcc) Fe to investigate the effects of Zn and Zn-Ni doping on the boundary energy and electronic structure. The obtained results indicate that the mismatch between the sizes of Zn and Fe atoms at the Zn-doped grain boundary causes its expansion, which increases the lengths of Fe-Fe bonds, leading to their weakening, and reduces the overall boundary strength. After the Zn doping of the Fe grain boundary, Zn atoms form covalent bonds with Fe atoms, that decreases the charge density of Fe-Fe bonds and their strength. Meanwhile, the strength of the newly formed Fe-Zn covalent bonds oriented at a certain angle with respect to the grain boundary direction is very low. The breakage of Fe-Fe bonds that occurs under tensile loading rapidly decreases the boundary strength. Finally, after the Zn-Ni co-doping of the Fe grain boundary, Ni atoms form metallic bonds with Fe atoms, thus increasing both the charge density of Fe-Fe bonds (as compared with that of the Fe-Fe bonds at the Zn-doped grain boundary).
TrpNet: Understanding Tryptophan Metabolism across Gut Microbiome
Authors: Lu Yao , Chong Jasmine , Shen Shiqian , Chammas Joey-Bahige , Chalifour Lorraine , Xia Jianguo
Published: December 2021

Crosstalk between the gut microbiome and the host plays an important role in animal development and health. Small compounds are key mediators in this host–gut microbiome dialogue. For instance, tryptophan metabolites, generated by biotransformation of tryptophan through complex host–microbiome co-metabolism can trigger immune, metabolic, and neuronal effects at local and distant sites. However, the origin of tryptophan metabolites and the underlying tryptophan metabolic pathway(s) are not well characterized in the current literature. A large number of the microbial contributors of tryptophan metabolism remain unknown, and there is a growing interest in predicting tryptophan metabolites for a given microbiome. Here, we introduce TrpNet, a comprehensive database and analytics platform dedicated to tryptophan metabolism within the context of host (human and mouse) and gut microbiome interactions. TrpNet contains data on tryptophan metabolism involving 130 reactions, 108 metabolites and 91 enzymes across 1246 human gut bacterial species and 88 mouse gut bacterial species. Users can browse, search, and highlight the tryptophan metabolic pathway, as well as predict tryptophan metabolites on the basis of a given taxonomy profile using a Bayesian logistic regression model. We validated our approach using two gut microbiome metabolomics studies and demonstrated that TrpNet was able to better predict alterations in in indole derivatives compared to other established methods.
Knockout of Arabidopsis thaliana VEP1, Encoding a PRISE (Progesterone 5β-Reductase/Iridoid Synthase-Like Enzyme), Leads to Metabolic Changes in Response to Exogenous Methyl Vinyl Ketone (MVK)
Authors: Klein Jan , Ernst Mona , Christmann Alexander , Tropper Marina , Leykauf Tim , Kreis Wolfgang , Munkert Jennifer
Published: December 2021

Small or specialized natural products (SNAPs) produced by plants vary greatly in structure and function, leading to selective advantages during evolution. With a limited number of genes available, a high promiscuity of the enzymes involved allows the generation of a broad range of SNAPs in complex metabolic networks. Comparative metabolic studies may help to understand why—or why not—certain SNAPs are produced in plants. Here, we used the wound-induced, vein patterning regulating VEP1 (AtStR1, At4g24220) and its paralogue gene on locus At5g58750 (AtStR2) from Arabidopsis to study this issue. The enzymes encoded by VEP1-like genes were clustered under the term PRISEs (progesterone 5β-reductase/iridoid synthase-like enzymes) as it was previously demonstrated that they are involved in cardenolide and/or iridoid biosynthesis in other plants. In order to further understand the general role of PRISEs and to detect additional more “accidental” roles we herein characterized A. thaliana steroid reductase 1 (AtStR1) and compared it to A. thaliana steroid reductase 2 (AtStR2). We used A. thaliana Col-0 wildtype plants as well as VEP1 knockout mutants and VEP1 knockout mutants overexpressing either AtStR1 or AtStR2 to investigate the effects on vein patterning and on the stress response after treatment with methyl vinyl ketone (MVK). Our results added evidence to the assumption that AtStR1 and AtStR2, as well as PRISEs in general, play specific roles in stress and defense situations and may be responsible for sudden metabolic shifts.
Measuring Livestock CH4 Emissions with the Laser Methane Detector: A Review
Authors: Sorg Diana
Published: December 2021

The handheld, portable laser methane detector (LMD) was developed to detect gas leaks in industry from a safe distance. Since 2009, it has also been used to measure the methane (CH4) concentration in the breath of cattle, sheep, and goats to quantify their CH4 emissions. As there is no consensus on a uniform measurement and data-analysis protocol with the LMD, this article discusses important aspects of the measurement, the data analysis, and the applications of the LMD based on the literature. These aspects, such as the distance to the animal or the activity of the animals, should be fixed for all measurements of an experiment, and if this is not possible, they should at least be documented and considered as fixed effects in the statistical analysis. Important steps in data processing are thorough quality control and reduction in records to a single point measurement or “phenotype” for later analysis. The LMD can be used to rank animals according to their CH4 breath concentration and to compare average CH4 production at the group level. This makes it suitable for genetic and nutritional studies and for characterising different breeds and husbandry systems. The limitations are the lower accuracy compared to other methods, as only CH4 concentration and not flux can be measured, and the high amount of work required for the measurement. However, due to its flexibility and non-invasiveness, the LMD can be an alternative in environments where other methods are not suitable or a complement to other methods. It would improve the applicability of the LMD method if there were a common protocol for measurement and data analysis developed jointly by a group of researchers.
Femtosecond-Laser-Ablation Dynamics in Silicon Revealed by Transient Reflectivity Change
Authors: Feng Tao , Chen Gong , Han Hainian , Qiao Jie
Published: December 2021

The dynamics of ablation in monocrystalline silicon, from electron-hole plasma generation to material expansion, upon irradiation by a single femtosecond laser pulse (1030 nm, 300 fs pulse duration) at a wide range of fluences is investigated using a time-resolved microscopy technique. The reflectivity evolution obtained from dynamic images in combination with a theoretical Drude model and a Two-Temperature model provides new insights on material excitation and ablation process. For all fluences, the reflectivity increased to a temporary stable state after hundreds of femtoseconds. This behavior was predicted using a temperature-dependent refractive index in the Drude model. The increase in velocity of plasma generation with increasing fluence was theoretically predicted by the Two-Temperature model. Two ablation regimes at high fluences (>0.86 J/cm2) were identified through the measured transient reflectivity and ablation crater profile. The simulation shows that the fluence triggering the second ablation regime produces a boiling temperature (silicon, 2628 K).
Recent Advances in Cryptococcus and Cryptococcosis
Authors: Firacative Carolina , Trilles Luciana , Meyer Wieland
Published: December 2021

The members of the Cryptococcus neoformans and Cryptococcus gattii species complexes are the main etiological agents of cryptococcosis, a life-threatening fungal infection affecting mostly immunocompromised people, but also immunocompetent hosts or those with unrecognized risk factors [...]
Plant Protein-Based Delivery Systems: An Emerging Approach for Increasing the Efficacy of Lipophilic Bioactive Compounds
Authors: Gomes Andresa , Sobral Paulo José do Amaral
Published: December 2021

The development of plant protein-based delivery systems to protect and control lipophilic bioactive compound delivery (such as vitamins, polyphenols, carotenoids, polyunsaturated fatty acids) has increased interest in food, nutraceutical, and pharmaceutical fields. The quite significant ascension of plant proteins from legumes, oil/edible seeds, nuts, tuber, and cereals is motivated by their eco-friendly, sustainable, and healthy profile compared with other sources. However, many challenges need to be overcome before their widespread use as raw material for carriers. Thus, modification approaches have been used to improve their techno-functionality and address their limitations, aiming to produce a new generation of plant-based carriers (hydrogels, emulsions, self-assembled structures, films). This paper addresses the advantages and challenges of using plant proteins and the effects of modification methods on their nutritional quality, bioactivity, and techno-functionalities. Furthermore, we review the recent progress in designing plant protein-based delivery systems, their main applications as carriers for lipophilic bioactive compounds, and the contribution of protein-bioactive compound interactions to the dynamics and structure of delivery systems. Expressive advances have been made in the plant protein area; however, new extraction/purification technologies and protein sources need to be found Their functional properties must also be deeply studied for the rational development of effective delivery platforms.
Anti-Inflammatory Activity of a CB2 Selective Cannabinoid Receptor Agonist: Signaling and Cytokines Release in Blood Mononuclear Cells
Authors: Capozzi Antonella , Caissutti Daniela , Mattei Vincenzo , Gado Francesca , Martellucci Stefano , Longo Agostina , Recalchi Serena , Manganelli Valeria , Riitano Gloria , Garofalo Tina , Sorice Maurizio , Manera Clementina , Misasi Roberta
Published: December 2021

The endocannabinoid system (ECS) exerts immunosuppressive effects, which are mostly mediated by cannabinoid receptor 2 (CBR2), whose expression on leukocytes is higher than CBR1, mainly localized in the brain. Targeted CBR2 activation could limit inflammation, avoiding CBR1-related psychoactive effects. Herein, we evaluated in vitro the biological activity of a novel, selective and high-affinity CBR2 agonist, called JT11, studying its potential CBR2-mediated anti-inflammatory effect. Trypan Blue and MTT assays were used to test the cytotoxic and anti-proliferative effect of JT11 in Jurkat cells. Its pro-apoptotic activity was investigated analyzing both cell cycle and poly PARP cleavage. Finally, we evaluated its impact on LPS-induced ERK1/2 and NF-kB-p65 activation, TNF-α, IL-1β, IL-6 and IL-8 release in peripheral blood mononuclear cells (PBMCs) from healthy donors. Selective CB2R antagonist SR144528 and CBR2 knockdown were used to further verify the selectivity of JT11. We confirmed selective CBR2 activation by JT11. JT11 regulated cell viability and proliferation through a CBR2-dependent mechanism in Jurkat cells, exhibiting a mild pro-apoptotic activity. Finally, it reduced LPS-induced ERK1/2 and NF-kB-p65 phosphorylation and pro-inflammatory cytokines release in human PBMCs, proving to possess in vitro anti-inflammatory properties. JT11 as CBR2 ligands could enhance ECS immunoregulatory activity and our results support the view that therapeutic strategies targeting CBR2 signaling could be promising for the treatment of chronic inflammatory diseases.
Chemical Profiling, Bioactivity Evaluation and the Discovery of a Novel Biopigment Produced by Penicillium purpurogenum CBS 113139
Authors: Tsiailanis Antonis D. , Pateraki Chrysanthi , Kyriazou Mary , Chatzigiannis Christos M. , Chatziathanasiadou Maria , Parisis Nikolaos , Mandala Ioanna , Tzakos Andreas G. , Koutinas Apostolis
Published: December 2021

Biobased pigments are environmentally friendly alternatives to synthetic variants with an increased market demand. Production of pigments via fermentation is a promising process, yet optimization of the production yield and rate is crucial. Herein, we evaluated the potential of Penicillium purpurogenum to produce biobased pigments. Optimum sugar concentration was 30 g/L and optimum C:N ratio was 36:1 resulting in the production of 4.1–4.5 AU (namely Pigment Complex A). Supplementation with ammonium nitrate resulted in the production of 4.1–4.9 AU (namely Pigment Complex B). Pigments showed excellent pH stability. The major biopigments in Pigment Complex A were N-threonyl-rubropunctamin or the acid form of PP-R (red pigment), N-GABA-PP-V (violet pigment), PP-O (orange pigment) and monascorubrin. In Pigment Complex B, a novel biopigment annotated as N-GLA-PP-V was identified. Its basic structure contains a polyketide azaphilone with the same carboxyl-monascorubramine base structure as PP-V (violet pigment) and γ-carboxyglutamic acid (GLA). The pigments were not cytotoxic up to 250 μg/mL.
Localized Surface Plasmon Resonance Decorated with Carbon Quantum Dots and Triangular Ag Nanoparticles for Chlorophyll Detection
Authors: Nazri Nur Afifah Ahmad , Azeman Nur Hidayah , Bakar Mohd Hafiz Abu , Mobarak Nadhratun Naiim , Luo Yunhan , Arsad Norhana , Aziz Tg Hasnan Tg Abd , Zain Ahmad Rifqi Md , Bakar Ahmad Ashrif A.
Published: December 2021

This paper demonstrates carbon quantum dots (CQDs) with triangular silver nanoparticles (AgNPs) as the sensing materials of localized surface plasmon resonance (LSPR) sensors for chlorophyll detection. The CQDs and AgNPs were prepared by a one-step hydrothermal process and a direct chemical reduction process, respectively. FTIR analysis shows that a CQD consists of NH2, OH, and COOH functional groups. The appearance of C=O and NH2 at 399.5 eV and 529.6 eV in XPS analysis indicates that functional groups are available for adsorption sites for chlorophyll interaction. A AgNP–CQD composite was coated on the glass slide surface using (3-aminopropyl) triethoxysilane (APTES) as a coupling agent and acted as the active sensing layer for chlorophyll detection. In LSPR sensing, the linear response detection for AgNP–CQD demonstrates R2 = 0.9581 and a sensitivity of 0.80 nm ppm−1, with a detection limit of 4.71 ppm ranging from 0.2 to 10.0 ppm. Meanwhile, a AgNP shows a linear response of R2 = 0.1541 and a sensitivity of 0.25 nm ppm−1, with the detection limit of 52.76 ppm upon exposure to chlorophyll. Based on these results, the AgNP–CQD composite shows a better linearity response and a higher sensitivity than bare AgNPs when exposed to chlorophyll, highlighting the potential of AgNP–CQD as a sensing material in this study.
Bisphenol A Adsorption on Silica Particles Modified with Beta-Cyclodextrins
Authors: Bucur Stefan , Diacon Aurel , Mangalagiu Ionel , Mocanu Alexandra , Rizea Florica , Dinescu Adrian , Ghebaur Adi , Boscornea Aurelian Cristian , Voicu Georgeta , Rusen Edina
Published: December 2021

This study presents the synthesis of silica particles bearing two beta-cyclodextrin (BCD) (beta-cyclodextrin-BCD-OH and diamino butane monosubstituted beta-cyclodextrin-BCD-NH2). The successful synthesis of the BCD-modified silica was confirmed by FT-IR and TGA. Using contact angle measurements, BET analysis and SEM characterization, a possible formation mechanism for the generation of silica particles bearing BCD derivatives on their surface was highlighted. The obtained modified silica displayed the capacity to remove bisphenol A (BPA) from wastewater due to the presence of the BCD moieties on the surface of the silica. The kinetic analysis showed that the adsorption reached equilibrium after 180 min for both materials with qe values of 107 mg BPA/g for SiO2-BCD-OH and 112 mg BPA/g for SiO2-BCD-NH2. The process followed Ho’s pseudo-second-order adsorption model sustaining the presence of adsorption sites with different activities. The fitting of the Freundlich isotherm model on the experimental results was also evaluated, confirming the BCD influence on the materials’ adsorption properties.
Spectroscopy of Filled Single-Walled Carbon Nanotubes
Authors: Kharlamova Marianna V. , Kramberger Christian
Published: December 2021

Many envisaged applications, such as nanoelectronics, photovoltaics, thermoelectric power generation, light-emission devices, energy storage and biomedicine, necessitate single-walled carbon nanotube (SWCNT) samples with specific uniform electronic properties. The precise investigation of the electronic properties of filled SWCNTs on a qualitative and quantitative level is conducted by optical absorption spectroscopy, Raman spectroscopy, photoemission spectroscopy and X-ray absorption spectroscopy. This review is dedicated to the description of the spectroscopic methods for the analysis of the electronic properties of filled SWCNTs. The basic principle and main features of SWCNTs as well as signatures of doping-induced modifications of the spectra of filled SWCNTs are discussed.
Effect of Electrolytic Medium on the Electrochemical Reduction of Graphene Oxide on Si(111) as Probed by XPS
Authors: Marrani Andrea G. , Motta Alessandro , Amato Francesco , Schrebler Ricardo , Zanoni Robertino , Dalchiele Enrique A.
Published: December 2021

The wafer-scale integration of graphene is of great importance in view of its numerous applications proposed or underway. A good graphene–silicon interface requires the fine control of several parameters and may turn into a high-cost material, suitable for the most advanced applications. Procedures that can be of great use for a wide range of applications are already available, but others are to be found, in order to modulate the offer of different types of materials, at different levels of sophistication and use. We have been exploring different electrochemical approaches over the last 5 years, starting from graphene oxide and resulting in graphene deposited on silicon-oriented surfaces, with the aim of understanding the reactions leading to the re-establishment of the graphene network. Here, we report how a proper choice of both the chemical environment and electrochemical conditions can lead to a more controlled and tunable graphene–Si(111) interface. This can also lead to a deeper understanding of the electrochemical reactions involved in the evolution of graphene oxide to graphene under electrochemical reduction. Results from XPS, the most suitable tool to follow the presence and fate of functional groups at the graphene surface, are reported, together with electrochemical and Raman findings.
Food Insecurity Is Associated with the Quality of Diet of Non-Institutionalized Older Adults from a Southern Chilean Commune: A Cross-Sectional Study
Authors: Segura-Badilla Orietta , Kammar-García Ashuin , Navarro-Cruz Addí Rhode , Araneda-Flores Jacqueline , Mancilla-Galindo Javier , Vera-López Obdulia , Lazcano-Hernández Martin , Quezada-Figueroa Gladys
Published: December 2021

As the population ages, greater attention to age-related health problems related to diet and lifestyles is needed. Here, we sought to evaluate the associations between demographic and clinical characteristics and food insecurity with the quality of diet of non-institutionalized elderly from a southern Chilean commune. We performed an analytical cross-sectional study in a sample of 376 older adults. Nutritional status was evaluated through anthropometric measurements. Quality of diet was determined by the healthy eating index (HEI), obtained through the frequency of consumption questionnaire. Socioeconomic, demographic, and lifestyle variables were also collected. Ordinal logistic and Poisson regression models were applied to study associations with quality of diet. The sample consisted of more women (81.6%) than men (18.4%). Most older adults were found to live in a situation of vulnerability or poverty (82.4%), with most having food security (65.7%). According to the HEI, only 14.1% had a good quality of diet, 83.8% had diet in need of improvement, and 2.1% had an unhealthy diet. There was an association of food insecurity and cardiovascular risk (according to waist circumference) with lower quality of diet categories. However, an association with the unhealthy quality of diet category was not confirmed with Poisson regression analysis, which was possibly due to the low number of subjects in that category (n = 8, 2.1%). Other modifiable factors like physical activity, hours of sleep, and polypharmacy were not associated with lower quality of diet categories. Socioeconomic status, which is a structural health determinant, was not associated with decreased quality of diet. Since this was a cross-sectional study performed on a small sample from a Chilean commune, directionality of associations cannot be discerned, and future longitudinal studies could aim to better characterize these associations in larger samples of elderly patients.
Strategies to Prevent Sarcopenia in the Aging Process: Role of Protein Intake and Exercise
Authors: Rogeri Patricia S. , Zanella Rudyard , Martins Gabriel L. , Garcia Matheus D. A. , Leite Geovana , Lugaresi Rebeca , Gasparini Sandro O. , Sperandio Giovana A. , Ferreira Luis Henrique B. , Souza-Junior Tacito P. , Lancha Antonio Herbert
Published: December 2021

Sarcopenia is one of the main issues associated with the process of aging. Characterized by muscle mass loss, it is triggered by several conditions, including sedentary habits and negative net protein balance. According to World Health Organization, it is expected a 38% increase in older individuals by 2025. Therefore, it is noteworthy to establish recommendations to prevent sarcopenia and several events and comorbidities associated with this health issue condition. In this review, we discuss the role of these factors, prevention strategies, and recommendations, with a focus on protein intake and exercise.
Understanding the Interactions of Happiness, Self-Rated Health, Mental Feelings, Habit of Eating Healthy and Sport/Activities: A Path Model for Abu Dhabi
Authors: Badri Masood A. , Alkhaili Mugheer , Aldhaheri Hamad , Alnahyan Hamdan , Yang Guang , Albahar Muna , Alrashdi Asma
Published: December 2021

Understanding the interactions between happiness, self-perception of health, healthy eating behaviors, physical activities, and psychological feelings or symptoms of mental health provides necessary inputs for social policymaking. Using data from the second cycle of the Abu Dhabi Quality of Life survey, this study examined a path analysis of Abu Dhabi residents’ nature of assimilations between these variables. The results point to the significant association between happiness and self-rated health. In addition, the results portray significant direct paths to happiness from three mental feeling variables—“feeling calm and peaceful”, “having lots of energy”, and “feeling downhearted and depressed”. The variable of “often feeling rushed or pressed for time” shows a direct path to self-rated health only. Eating healthy food is significantly associated with both happiness and self-perception of health. In addition, “often doing physical activities” positively influences happiness. The present study enhances and refines policymakers’ understanding of the considered factors on happiness and self-rated health with further elaborations of the mediating roles of specific well-being determinants. Limitations and future research directions are also discussed.
Prevalence, Risk Factors and Genotypes of Giardia duodenalis in Sheltered Dogs in Tuscany (Central Italy)
Authors: Agresti Andrea , Berrilli Federica , Maestrini Michela , Guadano Procesi Isabel , Loretti Enrico , Vonci Niccolò , Perrucci Stefania
Published: December 2021

In sheltered dogs, the prevalence of Giardia duodenalis is frequently high and may include potential zoonotic genotypes. The prevalence, genotypes and potential risk factors of G. duodenalis were assessed in 168 dogs from four kennels (Pistoia, Prato, Florence, Valdarno) in Tuscany, central Italy and compared with data from previous Italian studies. The prevalence of other intestinal parasites was also investigated. Individual dog faecal samples collected from each kennel were examined by parasitological techniques and a rapid immunoassay for the detection of G. duodenalis and Cryptosporidium faecal antigens. On Giardia-positive samples, molecular analysis was performed for genotype identification. Overall, 69 dogs scored positive for G. duodenalis (41%), but significant differences (p ≤ 0.05) were found among the four kennels and sampling seasons. The potentially zoonotic assemblages A and B and the canine-specific assemblage C (Pistoia: A-AII, B, C; Prato: A-AII, B; Florence: A-AII; Valdarno: A and C) were identified. Toxocara canis (8.9%), Trichuris vulpis (3.6%), hookworms (1.19%) and Cryptosporidium sp. (0.6%) were also identified. The high prevalence of G. duodenalis and the identification of potentially zoonotic genotypes in all examined kennels underline the need to improve routine parasite monitoring and control measures and to provide insights into the zoonotic potential of G. duodenalis.
Control Strategies to Cope with Late Wilt of Maize
Authors: Degani Ofir
Published: December 2021

Control of maize late wilt disease (LWD) has been at the forefront of research efforts since the discovery of the disease in the 1960s. The disease has become a major economic restraint in highly affected areas such as Egypt and Israel, and is of constant concern in other counties. LWD causes dehydration and collapsing at a late stage of maize cultivation, starting from the male flowering phase. The disease causal agent, Magnaporthiopsis maydis, is a seed- and soil-borne phytoparasitic fungus, penetrating the roots at sprouting, colonizing the vascular system without external symptoms, and spreading upwards in the xylem, eventually blocking the water supply to the plant’s upperparts. Nowadays, the disease’s control relies mostly on identifying and developing resistant maize cultivars. Still, host resistance can be limited because M. maydis undergoes pathogenic variations, and virulent strains can eventually overcome the host immunity. This alarming status is driving researchers to continue to seek other control methods. The current review will summarize the various strategies tested over the years to minimize the disease damage. These options include agricultural (crop rotation, cover crop, no-till, flooding the land before sowing, and balanced soil fertility), physical (solar heating), allelochemical, biological, and chemical interventions. Some of these methods have shown promising success, while others have contributed to our understanding of the disease development and the environmental and host-related factors that have shaped its outcome. The most updated global knowledge about LWD control will be presented, and knowledge gaps and future aims will be discussed.
Characterization of a Nigerian Lumpy Skin Disease Virus Isolate after Experimental Infection of Cattle
Authors: Wolff Janika , Tuppurainen Eeva , Adedeji Adeyinka , Meseko Clement , Asala Olayinka , Adole Jolly , Atai Rebecca , Dogonyaro Banenat , Globig Anja , Hoffmann Donata , Beer Martin , Hoffmann Bernd
Published: December 2021

Lumpy skin disease virus (LSDV), together with sheeppox virus and goatpox virus, belong to the genus Capripoxvirus within the family Poxviridae. Collectively, they are considered the most serious poxvirus diseases of agricultural livestock. Due to their severe clinical course and consequent loss of production, as well as high mortality of naïve small and large ruminant populations, they are known to have a significant impact on the economy and global trade restrictions of affected countries. Therefore, all capripox diseases are classified as notifiable under the guidelines of the World Organization of Animal Health (OIE). Since the 1970s, several outbreaks of LSD have been recorded in Nigeria. Until now, only a little information on the virus strains leading to the reported outbreaks have been published, dealing mainly with the phylogenetic relationship of those strains and the description of field outbreaks. During the present study, we experimentally infected cattle with a low-passage Nigerian LSDV strain isolated from a skin sample of LSD positive cattle in Nigeria in 2018. Clinical, molecular and serological data indicate that this LSDV isolate is highly pathogenic in cattle since it induced a severe clinical course and approximately 33% mortality in naïve Holstein Friesian cattle after experimental infection.
Lack of Evidence for an Association between Previous HEV Genotype-3 Exposure and Glomerulonephritis in General
Authors: Pischke Sven , Tamanaei Sarah , Mader Maria , Schulze zur Wiesch Julian , Petersen-Benz Christine , Haddad Munif , Addo Marylyn M. , Schmidt Tilman , Huber Tobias B. , Krebs Christian F. , Steinmetz Oliver M. , Turner Jan E. , Hoxha Elion , Horvatits Thomas
Published: December 2021

Among numerous other immune-mediated diseases, glomerulonephritis has also been suspected to be an extrahepatic manifestation of HEV infection. In this prospective study, we tested 108 patients with glomerulonephritis and 108 age- and sex-matched healthy controls at the University Hospital Hamburg Eppendorf, Hamburg, Germany, for anti-HEV IgG (Wantai test) as a marker for previous HEV exposure. A total of 24 patients (22%) tested positive for anti-HEV IgG. Males tended to be more frequently anti-HEV IgG positive (29%) in comparison to females (16%). However, this does not reach statistical significance (p = 0.07). Anti-HEV IgG positive patients were older in comparison to negative patients (mean 53 vs. 45 years, p = 0.05). The kidney function seems to be slightly decreased in anti-HEV IgG positive patients in comparison to and anti-HEV IgG negative patients basing on creatinine (p = 0.04) and glomerular filtration rate (GFR) (p = 0.05). Slightly higher values of bilirubin could be found in IgG positive patients (p = 0.04). Anti-HEV-IgG seropositivity rate (22%) in glomerulonephritis patients, did not differ significantly in comparison to an age- and sex-matched control cohort of healthy blood donors (31/108 positive, 29%). A total of 2/2 patients with membranoproliferative glomerulonephritis (MPGN) tested anti-HEV IgG positive (p = 0.002 in comparison to glomerulonephritis patients with other subtypes). In conclusion, our findings indicate that previous HEV exposure in a region where GT3 is endemic is not associated with glomerulonephritis in general. However, the subgroup of MPGN patients should be investigated in future studies. Furthermore, future studies are needed to investigate whether the observed association between anti-HEV IgG positivity and reduced GFR in glomerulonephritis patients is HEV associated or is an age-related effect.
Hourly Relationship between Reference Evapotranspiration and Shoot Shrinkage in Walnut Trees and Pomegranate under Deficit Irrigation
Authors: Salgado Eduardo , Livellara Nieggiorba , Chaigneau Esteban , Varas Fernando , Cuneo Italo F.
Published: December 2021

Diameter fluctuations of branches, shoots, or fruits are related to plant transpiration and water potential. In the past, several models have related dendrometric variables and evapotranspiration on a daily scale. However, trunk–branch shrinkage occurs only between dawn and midday, while evapotranspiration occurs most of the day from sunrise to sunset. Previous models have failed to incorporate this key fact. The objective of the present study was to assess the relationship of hourly daily shrinkage (HDS) between dawn and the next 4 h to the hourly reference evapotranspiration (EToh) of the same period in walnut trees and pomegranate plants under different irrigation regimes. Our data show that the relationship between EToh and HDS is much better than several previous models that included maximum daily shrinkage (MDS) and reference evapotranspiration (ETo). The novel slope analysis of the relationship between HDS versus time used here corresponds to the velocity at which the HDS occurs, which depends on the ETo intensity at that moment. This new method of analyzing this type of data calls for a revision of these models and sets a new baseline for future analysis.
Elemental Analysis and In Vitro Evaluation of Antibacterial and Antifungal Activities of Usnea barbata (L.) Weber ex F.H. Wigg from Călimani Mountains, Romania
Authors: Popovici Violeta , Bucur Laura , Calcan Suzana Ioana , Cucolea Elena Iulia , Costache Teodor , Rambu Dan , Schröder Verginica , Gîrd Cerasela Elena , Gherghel Daniela , Vochita Gabriela , Caraiane Aureliana , Badea Victoria
Published: December 2021

This study aims to complete our research on Usnea barbata (L.) Weber ex F.H. Wigg (U. barbata) from the Călimani Mountains, Romania, with an elemental analysis and to explore its antibacterial and antifungal potential. Thus, we analyzed twenty-three metals (Ca, Fe, Mg, Mn, Zn, Al, Ag, Ba, Co, Cr, Cu, Li, Ni, Tl, V, Mo, Pd, Pt, Sb, As, Pb, Cd, and Hg) in dried U. barbata lichen (dUB) by inductively coupled plasma mass spectrometry (ICP-MS). For the second study, we performed dried lichen extraction with five different solvents (ethyl acetate, acetone, ethanol, methanol, and water), obtaining five U. barbata dry extracts (UBDE). Then, using an adapted disc diffusion method (DDM), we examined their antimicrobial activity against seven bacterial species—four Gram-positive (Staphylococcus aureus, Enterococcus casseliflavus, Streptococcus pyogenes, and Streptococcus pneumoniae) and three Gram-negative (Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa)—and two fungi species (Candida albicans and Candida parapsilosis). Usnic acid (UA) was used as a positive control. The ICP-MS data showed a considerable Ca content (979.766 µg/g), followed by, in decreasing order, Mg, Mn, Al, Fe, and Zn. Other elements had low levels: Ba, Cu, Pb, and Cr (3.782–1.002 µg/g); insignificant amounts (<1 µg/g) of Hg and V were also found in dUB. The trace elements Ag, As, Cd, Co, Li, Tl, Mo, Pd, Pt, and Sb were below detection limits (<0.1 µg/g). The DDM results—expressed as the size (mm) of the inhibition zone diameter (IZs)—proved that the water extract did not have any inhibitory activity on any pathogens (IZs = 0 mm). Gram-positive bacteria displayed the most significant susceptibility to all other UBDE, with Enterococcus casseliflavus showing the highest level (IZs = 20–22 mm). The most susceptible Gram-negative bacterium was Pseudomonas aeruginosa (IZs = 16–20 mm); the others were insensitive to all U. barbata dry extracts (IZs = 0 mm). The inhibitory activity of UBDE and UA on Candida albicans was slightly higher than on Candida parapsilosis.
The Decrease of Leaf Dark Respiration during Water Stress Is Related to Leaf Non-Structural Carbohydrate Pool in Vitis vinifera L.
Authors: Tombesi Sergio , Frioni Tommaso , Grisafi Francesca , Sabbatini Paolo , Poni Stefano , Palliotti Alberto
Published: December 2021

Dark respiration (Rd) is a fundamental plant process used to gain biomass and maintain plant physiological activity. It accounts for the metabolization of a large share of the carbon fixed by photosynthesis. However, Rd during conditions of severe plant water stress is still poorly understood. The decrease in leaf transpiration increases temperature, one of the most important drivers of leaf Rd. On the other hand, water stress decreases the pool of leaf carbohydrates, which are the most important substrate for respiration. The aim of the present work was to determine the impact of water shortage on leaf Rd in grapevine and understand the driving factors in modulating leaf Rd response under plant water stress conditions. Water stressed vines had lower Rd as the water shortage severity increased. Rd was correlated with leaf temperature in well-watered vines. Instead, in water stressed vines, Rd correlated with leaf soluble sugars. The decrease of leaf Rd in water stressed vines was due to the decrease of leaf non-structural carbohydrate that, under water stress conditions, exerted a limiting effect on Rd.