bluuebunny/crossref_metadata_2025 huggingface dataset:
Semi-Physical Simulation of Fan Rotor Assembly Process Optimization for Unbalance Based on Reinforcement Learning
Authors: Zhang Huibin , Wang Mingwei , Li Zhiang , Zhou Jingtao , Zhang Kexin , Ma Xin , Wang Manxian
Published: June 2022

An aero engine fan rotor is composed of a multi-stage disk and multi-stage blades. Excessive unbalance of the aero engine fan rotor after assembly is the main cause of aero engine vibration. In the rotor assembly process, blade sequencing optimization and multi-stage blade set assembly phase optimization are important for reducing the overall rotor unbalance. To address this problem, this paper proposes a semi-physical simulation method based on reinforcement learning to optimize the balance in the fan rotor assembly process. Firstly, based on the mass moments of individual blades, the diagonal mass moment difference is introduced as a constraint to build a single-stage blade sorting optimization model, and reinforcement learning is used to find the optimal sorting path so that the balance of the single-stage blade after sorting is optimal. Then, on the basis of the initial unbalance of the disk and the unbalance of the single-stage blade set, a multi-stage blade assembly phase optimization model is established, and reinforcement learning is used to find the optimal assembly phase so that the overall balance of the rotor is optimal. Finally, based on the collection of data during the assembly of the rotor, the least-squares method is used to fit and calculate the real-time assembly unbalance to achieve a semi-physical simulation of the optimization of balance during the assembly process. The feasibility and effectiveness of the proposed method are verified by experiments.
Effect of No-Tillage Management on Soil Organic Matter and Net Greenhouse Gas Fluxes in a Rice-Oilseed Rape Cropping System
Authors: Zheng Huabin , Tang Xianliang , Wei Jiabin , Xu Huaqin , Zou Yingbin , Tang Qiyuan
Published: June 2022

No-tillage (NT) management is considered a leading approach for sustaining crop production and improving soil and environmental quality. Based on a long-term no-tillage experiment in a rice–oilseed rape cropping system, we examined differences in soil organic matter (SOM), soil microbial carbon (C) and nitrogen (N) content, and methane (CH4) and carbon dioxide (CO2) fluxes between NT and conventional tillage (CT) management. SOM under NT was 21.0 g kg–1, and a significant difference was detected between 2004 and 2016. SOM increased under NT and CT by averages of 0.60 and 0.32 g kg–1 year–1, respectively. Soil microbial C and N content were higher under CT than under NT. However, soil C:N ratios under NT were 17.4 and 9.7% higher than the CT, respectively, whereas soil microbial C:N ratios under NT were on average 9.47 and 9.70% higher. In addition, about 70% of CO2 net uptake and over 99% of net CH4 emissions occurred during the rice season in May–September in the rice–oilseed rape cropping system. Annual cumulative CH4 and daytime net ecosystem CO2 exchange (NEE) under NT was 1813.9 g CO2 equiv. m–2, 10.8% higher than that under CT. Our results suggest that a higher soil microbial C:N ratio and NEE (CH4 and daytime CO2) could contribute to increasing SOM/C in the surface soil under NT management.
Evaluation of the Egg Predator Blattisocius tarsalis (Mesostigmata: Blattisociidae) for the Biological Control of the Potato Tuber Moth Tecia solanivora under Storage Conditions
Authors: Gavara Jorge , Cabello Tomás , Gallego Juan Ramón , Hernández-Suarez Estrella , Piedra-Buena Díaz Ana
Published: June 2022

Tecia solanivora is the most prevalent pest causing damage to potato crops in fields in the Canary Islands, but even more so in the postharvest storage period. However, currently, there are no authorised chemical insecticides for potato storage facilities. Analysis of the viability of the predator mite Blattisocius tarsalis as a biological control agent for this moth was carried out. A study of the temperature effect showed B. tarsalis maintains predatory capacity in the range of 10–27 °C. Though predatory activity increases with temperature, no differences in mortality rates were observed between 10 and 20 °C (33.52 ± 2.44 and 40.14 ± 3.54% efficacy rate, respectively), nor between 25 and 27 °C (59.26 ± 4.59 and 75.19 ± 4.64% efficacy rate, respectively). Under microcosm conditions, at low pest infestation (10 eggs), B. tarsalis achieved the highest mortality of eggs at a density of 5 mites, with an efficacy rate of 91.67 ± 8.33%. At high infestation levels (50 eggs), maximum mortality was achieved with a density of 10 mites and efficacy of 98.52 ± 1.48%. The choice-assay showed no preference of B. tarsalis between T. solanivora and Phthorimaea operculella, suggesting this mite could be useful in mixed infestations of potato moths. The results show B. tarsalis is a very good candidate as a control agent in storage conditions and even in mixed infestations of T. solanivora and P. operculella.
Grassland Management Impact on Soil Degradation and Herbage Nutritional Value in a Temperate Humid Environment
Authors: Bogunovic Igor , Kljak Kristina , Dugan Ivan , Grbeša Darko , Telak Leon Josip , Duvnjak Marija , Kisic Ivica , Kapović Solomun Marijana , Pereira Paulo
Published: June 2022

Understanding the importance of grassland management is crucial for predicting the effects on forage production, pasture and ecosystem stability. Studies about the impact of grassland management in temperate humid environments on soil, erosion and aboveground biomass properties are lacking. This study investigates the effect of different grassland managements—no grazing, moderate grazing and heavy grazing—on soil properties, hydrological responses and herbage quality in an organic farm located in Croatia. The results showed that heavy grazing significantly increased soil compaction, structural deterioration, erosion and nutrient transport compared with no grazing. Heavily grazed plots had significantly higher soil organic matter and nutrient concentrations compared with no-grazing plots. Moderately grazed plots had the highest biomass production and the herbage with higher quality compared with other treatments. Significantly higher ash contents on heavily and moderately grazed plots were due to cow trampling. Cow grazing behaviour was a more important factor for plant regrowth and herbage quality than soil properties. Moderate grazing did not induce serious soil erosion problems or reduce soil productivity. Soil conservation measures should focus only on the heavily grazed areas and include the introduction of rotational grazing in combination with various strategies: excluding grazing, reseeding and increasing the diversity of resting areas.
Effects of Continuous Ridge Tillage at Two Fertilizer Depths on Microbial Community Structure and Rice Yield
Authors: Liu Lihua , Cui Shize , Qin Meng , Chen Liqiang , Yin Dawei , Guo Xiaohong , Li Hongyu , Zheng Guiping
Published: June 2022

Ridge tillage at two fertilizer depths is a new type of conservation tillage method that was previously shown to substantially improve rice yield. This study aimed to compare the effects of continuous ridge tillage at two fertilizer depths (L treatment) with those of conventional cultivation (P treatment) on bacterial and fungal diversity in the rice root zone and study the correlation between microorganisms and yield components. At the mature stage, the yield and yield components of rice plants were compared. Test soil (0–20 cm) with continuous tillage for 3 years was used for high-throughput sequencing to analyze the microbial community structure in the root–soil of the two treatments. We found that the L treatment increased soil nutrient content and improved soil physical properties, which altered the composition of the microbial community. The bacterial ACE and Chao indices in the L treatment increased by 1.46% and 1.83%, respectively, and the fungal ACE and Chao indices increased by 5.25% and 5.49%, compared with the P treatment, respectively. The average theoretical yield under the L treatment was 9781.51 kg/ha, which was 19.23% higher than that under the P treatment. Continuous ridge tillage at two fertilizer depths can provide a better soil environment for rice growth and increase the yield.
Weeding Effectiveness and Changes in Soil Physical Properties Using Inter-Row Hoeing and a Robot
Authors: Bručienė Indrė , Buragienė Sidona , Šarauskis Egidijus
Published: June 2022

Weed control is one of the most important technological operations to ensure crop yield and quality in ecological sugar beet production. However, conventional mechanical weed control is labor- and time-intensive and has adverse effects on the soil and the environment. The aim of this study was to experimentally investigate the influence of conventional mechanical and robotic weed control systems on soil properties and to assess the effectiveness of these different weed control methods in ecological sugar beet production. This study examines two different weed control systems: robotic weed control (RWC) and conventional weed control (CWC). Field experimental studies were carried out with a solar-powered field robot and conventional inter-row cultivation (CWC1—first cultivation, CWC2—second cultivation) to determine the effectiveness of mechanical weed control in ecological sugar beet crops. The influence of different weed control systems on the physical properties of the soil in the contact zone between the soil and the tires of weed control machines was investigated. The results showed that the average weed control effectiveness inter-row was higher in the RWC (81%) compared to that in the CWC2 (46%). The overall weed control effectiveness of the robotic weed control in the sugar beet inter-row and intra-row was around 49%. The measurements showed that the weed control process reduced the soil moisture and temperature in all treatments tested. Experimental studies have confirmed that the weed control operation, although carried out with relatively lightweight robots, also has an impact on soil bulk density. RWC weed control resulted in an average increase of 0.16 g cm–3 in soil bulk density in the topsoil layer (0–10 cm) after weeding. Both CWC1 and RWC increased soil penetration resistance (PR). For CWC1, the average increase in topsoil PR after weed control was 20%, while for RWC, the increase was marginal, only around 1%. Automated precision weed control by self-propelled solar-powered field robots is an important solution to reduce the need for tedious and time-consuming manual weeding.
Assessment of Injury by Four Major Pests in Soybean Plants Using Hyperspectral Proximal Imaging
Authors: Iost Filho Fernando Henrique , de Bastos Pazini Juliano , de Medeiros André Dantas , Rosalen David Luciano , Yamamoto Pedro Takao
Published: June 2022

Arthropod pests are among the major problems in soybean production and regular field sampling is required as a basis for decision-making for control. However, traditional sampling methods are laborious and time-consuming. Therefore, our goal is to evaluate hyperspectral remote sensing as a tool to establish reflectance patterns from soybean plants infested by various densities of two species of stinkbugs (Euschistus heros and Diceraeus melacanthus (Hemiptera: Pentatomidae)) and two species of caterpillars (Spodoptera eridania and Chrysodeixis includens (Lepidoptera: Noctuidae)). Bioassays were carried out in greenhouses with potted plants placed in cages with 5 plants infested with 0, 2, 5, and 10 insects. Plants were classified according to their reflectance, based on the acquisition of spectral data before and after infestation, using a hyperspectral push-broom spectral camera. Infestation by stinkbugs did not cause significative differences in the reflectance patterns of infested or non-infested plants. In contrast, caterpillars caused changes in the reflectance patterns, which were classified using a deep-learning approach based on a multilayer perceptron artificial neural network. High accuracies were achieved when the models classified low (0 + 2) or high (5 + 10) infestation and presence or absence of insects. This study provides an initial assessment to apply a non-invasive detection method to monitor caterpillars in soybean before causing economic damage.
Heritability of Morphophysiological Traits in Popcorn for Drought Tolerance and Their Use as Breeding Indicators of Superior Genotypes
Authors: Viana Flávia Nicácio , Chaves Marcelo Moura , Kamphorst Samuel Henrique , do Amaral Junior Antônio Teixeira , Lima Valter Jário de , Leite Jhean Torres , Schmidt Katia Fabiane Medeiros , Oliveira Uéliton Alves de , Lamego Danielle Leal , Pereira Jacymara Lopes , Pena Guilherme Ferreira , Vieira Henrique Duarte , Oliveira Jurandi Gonçalves de , Daher Rogério Figueiredo , Campostrini Eliemar , Bressan-Smith Ricardo
Published: June 2022

This study aimed to identify the genetic control of morphological, water status, and root traits in four inbred lines and their pre-selected hybrid combinations of popcorn, and to identify the best parents and hybrids. The plants were grown under greenhouse conditions, and with two water conditions—water-stressed (WS) and well-watered (WW). We evaluated shoot biomass (SB), whole plant leaf area (WPLA), chlorophyll content, carbon isotope discrimination, net CO2 assimilation rate, the stomatal conductance, transpiration, cumulative plant transpiration, and root weight density in three sections. The reductions in SB and WPLA are attributed to stomatal causes, given the maintenance of water condition values in water-stressed plants. In fact, the stomata were closed for long periods during plant growth under the water-stressed condition, as indicated by the more negative carbon isotope discrimination values. The root weight density, regardless of soil depth and water conditions, was higher in hybrids. There was a preponderance of non-additive effects in the control of morphological and root traits, especially in the WS condition, and heterosis was shown to be the fundamental strategy for obtaining superior hybrids. For water status traits, it is essential to choose the female parent in the crossing block, given the maternal effect expressed by net CO2 assimilation rate, stomatal conductance, and transpiration.
VICAL: Global Calculator to Estimate Vegetation Indices for Agricultural Areas with Landsat and Sentinel-2 Data
Authors: Jiménez-Jiménez Sergio Iván , Marcial-Pablo Mariana de Jesús , Ojeda-Bustamante Waldo , Sifuentes-Ibarra Ernesto , Inzunza-Ibarra Marco Antonio , Sánchez-Cohen Ignacio
Published: June 2022

The vegetation indices (VIs) estimated from remotely sensed data are simple and based on effective algorithms for quantitative and qualitative evaluations of the dynamics of biophysical crop variables such as vegetation cover, leaf area, vigor and development, and many others. Over the last decade, many VIs have been proposed and validated to enhance the vegetation signal by reducing the noise from effects produced either by the soil or by vegetation such as brightness, shadows, color, etc. VIs are commonly calculated from satellite images such as ones from Landsat and Sentinel-2 because of their medium resolution and free availability. However, despite the VIs being fairly simple algorithms, it can take hours to calculate them for an established agricultural area, mainly due to the pre-processing of the images (including atmospheric corrections, the detection of clouds and shadows), size and download time of the images, and the capacity of the computer equipment used. Time increases as the number of images increases. In this sense, the free to use Google Earth Engine (GEE) platform was here used to develop an application called VICAL to calculate 23 VIs map (VIs commonly used in agricultural applications) and time series of any agricultural area in the world with images (cloud-free) from Landsat and Sentinel-2 data. It was found that VICAL can calculate these 23 VIs accurately, and shows the potential of the GEE cloud-based tools using multispectral dataset to assess many spectral VIs. This tool is very beneficial for researchers with poor access to satellite data or in institutions with a lack of computational infrastructure to handle the large volumes of satellite datasets, since it is not necessary for the user writing a single line of code. The VICAL is open-access image analysis platform that can be modified to carry out more complex analysis or adapt it to a specific VI application.
A Study on Long-Close Distance Coordination Control Strategy for Litchi Picking
Authors: Wang Hongjun , Lin Yiyan , Xu Xiujin , Chen Zhaoyi , Wu Zihao , Tang Yunchao
Published: June 2022

For the automated robotic picking of bunch-type fruit, the strategy is to roughly determine the location of the bunches, plan the picking route from a remote location, and then locate the picking point precisely at a more appropriate, closer location. The latter can reduce the amount of information to be processed and obtain more precise and detailed features, thus improving the accuracy of the vision system. In this study, a long-close distance coordination control strategy for a litchi picking robot was proposed based on an Intel Realsense D435i camera combined with a point cloud map collected by the camera. The YOLOv5 object detection network and DBSCAN point cloud clustering method were used to determine the location of bunch fruits at a long distance to then deduce the sequence of picking. After reaching the close-distance position, the Mask RCNN instance segmentation method was used to segment the more distinctive bifurcate stems in the field of view. By processing segmentation masks, a dual reference model of “Point + Line” was proposed, which guided picking by the robotic arm. Compared with existing studies, this strategy took into account the advantages and disadvantages of depth cameras. By experimenting with the complete process, the density-clustering approach in long distance was able to classify different bunches at a closer distance, while a success rate of 88.46% was achieved during fruit-bearing branch locating. This was an exploratory work that provided a theoretical and technical reference for future research on fruit-picking robots.
The Effect of the Nitrogen-Fixing Bacteria and Companion Red Clover on the Total Protein Content and Yield of the Grain of Spring Barley Grown in a System of Organic Agriculture
Authors: Płaza Anna , Niewiadomska Alicja , Górski Rafał , Rudziński Robert , Rzążewska Emilia
Published: June 2022

Field research was conducted in Poland from 2019–2021 to determine the effect of the bacteria Azospirillumlipoferum Br17 and Azotobacter chroococcum, as well as companion red clover on the total protein content and yield in the grain of spring barley cultivated in a system of organic agriculture. Two factors were examined in the field experiment: I. bacterial formulations: 1—control, 2—nitrogen-fixing bacteria (Azospirillumlipoferum Br17, Azotobacter chroococcum), 3—nitrogen-fixing bacteria (Azospirillumlipoferum Br17, Azotobacter chroococcum) + phosphorus-releasing bacteria (Bacillus megaterium var. phosphaticum, Arthrobacter agilis), and 4—nitrogen-fixing bacteria (Azotobacter chroococcum) + plant growth-promoting rhizobacteria (PGPR) (Bacillus subtilis, Bacillus amyloliquefaciens, Pseudomonas fluorescens); II. companion crop: control without a companion crop, red clover, and red clover + Italian ryegrass. In spring barley grain, the total protein content was determined and the total protein yield was calculated. The obtained study results demonstrated that the growing season conditions significantly affected the total protein content and yield in the spring barley grain. The highest total protein content was recorded in the grain of spring barley following an application of nitrogen-fixing bacteria (Azotobacter chroococcum) combined with PGPR (Bacillus subtilis, Pseudomonas fluorescens) and grown with companion red clover.
Effect of Six Different Feedstocks on Biochar’s Properties and Expected Stability
Authors: Bednik Magdalena , Medyńska-Juraszek Agnieszka , Ćwieląg-Piasecka Irmina
Published: June 2022

Biochar (BC) is often proposed as a tool for climate change mitigation, due to the expected long lifetime in the environment. However, BC’s stability can vary depending on feedstock type and the presence of labile carbon fractions. In this study, we verify the recent methods with new possible tools for biochar stability assessment on six different biochars derived from commonly available Europe biomass sources. Elemental composition (CHNO), dissolved organic carbon (DOC) and water-soluble carbonates content (WSC), volatile organic compounds (VOCs) composition, and mid-infrared spectra (MIR) were performed to estimate the persistence of biochars. Under similar conditions of pyrolysis, biochar properties can vary depending on a feedstock origin. Less aromatic structure and higher contents of labile carbon fractions (DOCs and WSC) in food waste biochars affected the lower stability, while biochars derived from high lignocellulose materials (straw, wood, and grass) were strongly carbonized, with persistent, aromatic structure. Labile carbon pool content (DOC, WSC) and spectral analysis can be useful tools for biochar stability assessment, giving similar information to the standard molar ratio method. Biochars obtained from agriculture and forestry management biomass should be considered as highly stable in soil and are appropriate for long-term carbon sequestration purposes.
Life Cycle Assessment of the Cultivation Processes for the Main Vegetable Crops in Southern Egypt
Authors: Abdelkader Mostafa , Zargar Meisam , Murtazova Kheda Magomed-Salihovna , Nakhaev Magomed Ramzanovich
Published: June 2022

Due to the increasing concern about climate change and environmental sustainability, the investigation of energy consumption represents a very intriguing and undeniable subject. This study was directed to investigate energy footprints, greenhouse gas (GHG) emissions and life cycle assessment (LCA) of the main vegetable crops cultivated under open field conditions in southern Egypt. Potato production required the maximum energy amount (112.3 GJ/ha) compared to 76 GJ and 96 GJ for onion and tomato, respectively. Based on energy indices, potato gave (energy ratio > 1; energy productivity > 1; energy profitability > 1; net energy > 0), while onion and tomato production shared the same indicators (energy ratio < 1; energy productivity > 1; energy profitability < 0; net energy < 0). However, GHG emissions generated for producing one ton of potato tubers registered the least amount by 76.0 kg CO2 eq. The same GHG amount was produced by 834 kg of onion bulbs and 940.6 kg of tomato fruits. The emission rates were more a consequence of diesel, followed by inorganic fertilizer and manure. In addition to carbon emissions, every production process causes several other environmental problems, thus a comprehensive analysis of environmental impact categories is required. The openLCA program performed LCA and ten impact categories were considered to transform the inventory data into several indicators. Producing one ton of potato tubers has the least footprint on the environment and the ecosystem, such as global warming (GW)—238.8 kg CO2 eq. t−1; human toxicity (HT)—288.3 kg 1,4-DB eq. t−1; fresh water aquatic ecotoxicity (FAEF)—160.44 kg 1,4-DB eq. t−1; marine aquatic ecotoxicity (MAET)—365,636 kg 1,4-DB eq. t−1; and terrestrial ecotoxicity (TE)—1.18 kg 1,4-DB eq. t−1. The analyses indicated that machinery and diesel fuel had the highest impact on all the studied categories.
Silicon Fertilizer and Microbial Agents Changed the Bacterial Community in the Consecutive Replant Soil of Lilies
Authors: Yu Yanlin , Zhang Lipeng , Li Yuanpeng , Hou Lei , Yang Hongyu , Shi Guiying
Published: June 2022

Crop replanting leads to soil degradation and soil productivity reduction, which is a challenge for sustainable agricultural development. We previously found that silicon fertilizers combined with additional microbial agents are an effective means to alleviate problems that occur in a variety of Chinese lily during replanting, but little is known about the changes in microbial structure during this process. In the present study, we applied four treatments: CK (control), SF (silicon fertilizer), MF (microbial agents), and SMF (combination of silicon fertilizer and microbial agents). We treated the soil constantly for three years and investigated the bacterial community structure and some specific microbial groups in the soil of the lily root zone using 16S rRNA high-throughput sequencing analysis. The results showed that silicon fertilizer and microbial agent treatment significantly improved the growth status of the plants and changed the diversity and structure of the bacterial community in the soil. The genus Pseudomonas significantly increased in the SF treatment, and the phylum Actinobacteria and the genera Nordella, Devosia, and Rhodoplanes significantly increased in the SMF treatment. The genera Nordella, Pedomicrobium, and Chthoniobacter correlated with the seedling index or available silicon content. In addition, the two genera Gaiella and Nocardioides were the key species linking the bacterial community in the soil. The soil physicochemical properties played an important role in restoring the soil bacterial community structures. In conclusion, silicon fertilizer and microbial agents changed the diversity and structure of the bacterial community. Under the fertilizer supplement model, the enrichment of the phylum Actinobacteria and the genus Pseudomonas played an important role in improving soil health and alleviating CRPs in lilies. In addition, organic matter, available phosphorus, available potassium, and available silicon were found to be the most important factors that have a great impact on the restoration of bacterial community structures.
Relationship between the Composition of Lipids in Forages and the Concentration of Conjugated Linoleic Acid in Cow’s Milk: A Review
Authors: Acosta Balcazar Isabel Cristina , Granados Rivera Lorenzo Danilo , Salinas Chavira Jaime , Estrada Drouaillet Benigno , Albarrán Miguel Ruiz , Bautista Martínez Yuridia
Published: June 2022

Conjugated linoleic acid (CLA), has been shown to have protective effects against various diseases, such as obesity, arteriosclerosis, diabetes, chronic inflammatory diseases, and cancer. This fatty acid in ruminants results from two processes, biohydrogenation, which takes place in the rumen, and de novo synthesis, carried out in the mammary gland, and it has linoleic and α-linolenic acids as its precursors. The amounts of precursors in the diets of animals are related to the amounts of CLA in milk. In the literature review, it was found that the milk of cows fed fresh forage has a higher amount of CLA because they have a higher amount of linoleic acid and α-linolenic acid compared to other foods used in the diets of cows. The amount of CLA precursors in pastures can be increased through agronomic practices, such as nitrogen fertilization, and regrowth age. It is also a technique used to increase the amount of CLA in milk to obtain a greater benefit regarding its nutritional value.
The Impact of COVID-19 Zoo Closures on Behavioural and Physiological Parameters of Welfare in Primates
Authors: Williams Ellen , Carter Anne , Rendle Jessica , Fontani Sara , Walsh Naomi Davies , Armstrong Sarah , Hickman Sarah , Vaglio Stefano , Ward Samantha J.
Published: June 2022

Primates are some of the most cognitively advanced species held in zoos, and their interactions with visitors are complex. The COVID-19 pandemic provided a unique opportunity to understand the impact of zoo visitors on animals, in comparison to “empty zoos”. This study sought to understand the impact of facility closures and subsequent reopenings on behavioural and physiological parameters of welfare in four primate species housed in the UK: bonobos (Pan paniscus) (n = 8), chimpanzees (Pan troglodytes) (n = 11), and western lowland gorillas (Gorilla gorilla gorilla) (n = 6) held at Twycross Zoo (TZ); and olive baboons (Papio anubis) (n = 192) held at Knowsley Safari (KS). Behavioural data were collected from April–September 2020 (KS) and November 2020–January 2021 (TZ). Faecal samples were collected during morning checks from October–November (TZ) and July–November 2020 (KS). Faecal glucocorticoid metabolites (FGMs) were measured using ELISA kits. Statistical analysis for behavioural observations was undertaken using general linear models. Enclosure usage was assessed using t-tests and Mann–Whitney U-tests as appropriate. Bonobos and gorillas spent less time alone when facilities were open to the public (p = 0.004, p = 0.02 respectively). Gorillas spent less time resting when the facility was open to the public (p = 0.04), and chimpanzees engaged in more feeding (p = 0.02) and engagement with enrichment (p = 0.03) when the zoo was open to the public than when it was closed. Olive baboons performed less sexual and dominance behaviour and approached visitor cars more frequently when the safari park was opened to the public than they did the ranger’s vehicle during closure periods. There were no significant changes in physiological parameters for any of the study species. The results suggest variable impacts of the zoo closures on zoo-housed primates. We recommend future work that seeks to understand the impact of individual-level differences on “visitor effects” and that differences between animal experiences in zoos and safari parks are further explored in a range of species.
Supplementation of Broiler Chicken Feed Mixtures with Micronised Oilseeds and the Effects on Nutrient Contents and Mineral Profiles of Meat and Some Organs, Carcass Composition Parameters, and Health Status
Authors: Zając Malwina , Kiczorowska Bożena , Samolińska Wioletta , Klebaniuk Renata , Andrejko Dariusz , Kiczorowski Piotr , Milewski Szymon , Winiarska-Mieczan Anna
Published: June 2022

In this study, we included 15% doses of infrared-irradiated camelina, flax, and sunflower seeds in the diets of broiler chickens (grower and finisher) and focused on assessing the effects on the production traits, selected slaughter analysis parameters, nutrients, and minerals in breast and drumstick meat and some organs, i.e., liver, proventriculus, and heart. In total, 200 one-day-old broiler chickens were assigned randomly to four treatments with five replicate cages of 10 broiler chickens per cage (five females and five males). The experiment lasted 6 weeks. In the group of broiler chickens in which diets were supplemented with micronised camelina and flax seeds, there was an increase in (p < 0.05) breast, thigh, and drumstick weight and a decrease (p < 0.05) in the abdominal fat of the carcasses. The oilseed treatments reduced the ether extract content and the calorific value in the breast and drumstick muscles and organs. The flax seeds contributed to an increase in the contents of Ca (breast muscle and liver), Cu (breast muscle and proventriculus), and Fe (drumstick muscle and heart). Likewise, some blood parameters were influenced by supplementation with infrared-irradiated camelina and flax seeds, for example, there was a decrease in the haemoglobin level and the mean corpuscular haemoglobin concentration (p < 0.05). The oilseed treatments also modified the contents of Fe and Ca in the blood plasma of broiler chickens (p < 0.05). It may be concluded that infrared-irradiated camelina, flax, and sunflower seeds can be regarded as good diet components exerting positive effects on the dietary value of poultry meat and organs used in dietetics.
Diversity, Distribution, and Biogeography of Freshwater Fishes in Guangxi, China
Authors: He Jiayang , Wu Zhiqiang , Huang Liangliang , Gao Minhui , Liu Hao , Sun Yangyan , Rad Saeed , Du Lina
Published: June 2022

The Guangxi Zhuang Autonomous Region has the largest number of cavefish species in the world and is a global biodiversity hotspot. In this study, a species list of freshwater fishes in 12 Sub−basins of Guangxi was compiled systematically. Moreover, the species composition and distribution of the diversity were analyzed via the G-F index, taxonomic diversity index, and beta diversity index. Results showed that 380 species of freshwater fishes were recorded in this region, which belonged to 158 genera in 43 families and 17 orders in 2 phyla, in which 128 species of endemic fishes and 83 species of cavefish accounted for 33.68% and 21.84%, respectively. The species diversity from northwest to southeast gradually decreased for most Sub−basins. The G-F index has generally risen in recent years. The taxonomic diversity index showed that the freshwater fish taxonomic composition in Guangxi is uneven. The spatial turnover component was the main contributor to beta diversity. A cluster analysis showed that the 12 Sub−basins in the study area could be divided into four groups, and the phylogenetic relationships of freshwater fishes in Guangxi generally reflect the connections between water systems and geological history. The freshwater fish system in Guangxi, which belonged to the South China division in the Southeast Asiatic subregion of the Oriental region, originated in the early Tertiary period. The results will provide the information needed for freshwater fish resource protection in Guangxi and a reference for promoting the normalization of fish diversity conservation in the Pearl River Basin and other basins.
Validation of an Enzyme Immunoassay to Measure Faecal Glucocorticoid Metabolites in Common Brushtail Possums (Trichosurus vulpecula) to Evaluate Responses to Rehabilitation
Authors: Cope Holly R. , Keeley Tamara , Keong Joy , Smith Daniel , Silva Fabiola R. O. , McArthur Clare , Webster Koa N. , Mella Valentina S. A. , Herbert Catherine A.
Published: June 2022

Volunteer wildlife rehabilitators rescue and rehabilitate thousands of native animals every year in Australia. However, there is little known about how exposure to novel stimuli during rehabilitation could affect the physiology of wildlife. We investigated this question in a species that commonly enters rehabilitation, the common brushtail possum (Trichosurus vulpecula). We evaluated five enzyme immunoassays (EIA) to determine the most suitable for measuring faecal glucocorticoid metabolites (FGM) as a proxy for evaluating the response of brushtail possums to potential stressors during rehabilitation. An adrenocorticotrophin hormone (ACTH) challenge was conducted on wild-caught possums to determine the best-performing EIA based on the successful detection of FGM peaks in at least two of three possums. While a number of assays met these criteria, the 11-oxoaetiocholanolone (abbreviation: 72a) EIA was selected as it had the largest amplitude of change in response to the ACTH challenge. This assay was then used to measure FGM concentrations in 20 possums during rehabilitation. There was high variation in baseline FGM concentrations and response to captivity between possums. Significant changes in FGM levels were detected in most possums during captivity, but were not reliably associated with potentially stressful events that were identified by rehabilitators. The probability of an FGM peak occurring within five days of a potentially stressful event was about 50%, regardless of the type of event. Our study has demonstrated that injured and orphaned possums show changes in FGMs during captivity and rehabilitation and has identified events that can induce a physiological response in some individuals. We recommend that research now focus on the relationship between these responses during rehabilitation and pre- and post-release survival.
Evaluation of Two Equations for Prediction of Digestible Energy in Mixed Feeds and Diets for Horses
Authors: Martínez Marín Andrés Luis , Valle Emanuela , Bergero Domenico , Requena Francisco , Forte Claudio , Schiavone Achille
Published: June 2022

Several authors have developed equations for estimating digestible energy in horse feeds as an alternative to the inconveniences of in vivo digestibility assays. We aimed to evaluate two of such equations. A dataset was constructed from the literature with 32 mixed feeds and diets of known proximate composition, whose digestibility was measured in in vivo assays. Then, the digestible energy of the mixed feeds and diets was predicted with both equations from their proximate components. Precision, accuracy, reproducibility, bias, and decomposition of total error of predictions were determined. Both equations performed almost equally well (R2 = 0.89 vs. 0.87, root mean square error of prediction = 183 vs. 217 kcal/kg dry matter, concordance correlation coefficient = 0.91 vs. 0.86, and linear error = 24.6 vs. 33.6% of total error). Linear bias (p < 0.01 in both equations) resulted in overvaluation of low digestible energy feeds and, to a lesser extent, undervaluation of high digestible energy feeds and was significantly (p < 0.05) related to crude fiber. The obtained results indicate that the accuracy of both equations could be improved by reassessing the effects of crude fiber on the digestibility of the other proximate components.
Locally Grown Crops and Immunocastration in Fattening Heavy Pigs: Effects on Performance and Welfare
Authors: Argemí-Armengol Immaculada , Villalba Daniel , Vall Laura , Coma Ramon , Roma Josep , Álvarez-Rodríguez Javier
Published: June 2022

This study aimed to explore dietary replacement soybean meal (SBM) with local pea seeds (PS-L) and the effects of surgically castrated (CM) or immunocastration (IM) in heavy male pigs, on growth performance, feeding behaviour, and tail and ear lesions. Four treatments were arranged factorially (2 × 2), with two sexes and two dietary treatments (96 pigs in eight pens). The inclusion of PS-L was 25%, 30%, and 40% during three phases (40–80 kg, 80–110 kg, and 110–140 kg, respectively). No difference in average daily feed intake (ADFI) and body-weight (BW) between PS-L and SBM could be demonstrated (p > 0.05), but PS-L diet decreased the average daily gain (ADG) at 110–140 kg of BW (p < 0.001) and increased feed conversion rate (FCR) (p < 0.05). The ADG was higher (p < 0.01) in IM than CM in all fattening periods, and the FCR in IM was lower (p < 0.05) than in barrows. IM pigs had lower dressing percentage than CM (p < 0.01). Pigs fed a PS-L diet ate faster but increased their passive behaviour compared with those fed SBM. In conclusion, the PS-L diet did not reduce BW and improved passive behaviour, and IM grew more efficiently, regardless of diet.
Alternative Molecular Tools for the Fight against Infectious Diseases of Small Ruminants: Native Sicilian Sheep Breeds and Maedi-Visna Genetic Susceptibility
Authors: Tumino Serena , Tolone Marco , Galluzzo Paola , Migliore Sergio , Sechi Tiziana , Bordonaro Salvatore , Puleio Roberto , Carta Antonello , Loria Guido Ruggero
Published: June 2022

Maedi-visna (MV) is a disease caused by small ruminant lentiviruses. It is included in the list of notifiable terrestrial animal diseases due to economic losses and animal welfare harm in the sheep sector. To date, control programs remain the onliest approach to avoiding infection. The allelic variant p.Glu35Lys (E35K) of the TMEM154 gene has been strongly associated with host vulnerability to MV illness. The present study aimed to investigate the association of TMEM154 E35K allele frequencies with MV susceptibility in native Sicilian sheep breeds. More than 400 animals from 14 local sheep were serologically tested and genotyped for the TMEM154 E35K polymorphism. The local breeds displayed different values of MV seroprevalence, with the lowest antibody prevalence in Barbaresca and Pinzirita breeds. TMEM154 protective allele (K35) was less frequent than the risk allele (E35) in Valle del Belìce breed, whereas the other three breeds showed a more balanced alleles distribution. A positive association between seroprevalence and genotype was found in the entire sample set. The risk of infection resulted in more than 3-fold times as high in sheep with EK and EE genotype compared to the KK genotype. Our data could be helpful in establishing selection breeding programs aimed at reducing MV infection in Sicilian sheep farming and encouraging the breeding of native breeds.
Effect of Femoral Head and Neck Osteotomy on Canines’ Functional Pelvic Position and Locomotion
Authors: Engstig Madeleine , Vesterinen Senni , Morelius Mikael , Junnila Jouni , Hyytiäinen Heli K.
Published: June 2022

The long-term effect of femoral head and neck osteotomy (FHO) on the locomotory system of dogs was evaluated. The study comprised an owner questionnaire and an orthopaedic examination, anatomical measurements, and pressure-sensitive walkway analysis for dogs. Linear mixed effect models were used for statistical analysis. Ten dogs with a median of 2.5 years since their unilateral FHO were included. According to the questionnaire results, nine dogs had returned to a normal physical activity level. Muscle atrophy (p = 0.005), less extension in the coxofemoral joint (p = 0.003), and less static weight bearing on the FHO limb (p = 0.003) were observed. No consistent pattern regarding tilt or position of the pelvis was noted when measuring height of the tuber ischii (p = 0.39). Five of the dogs tilted away from, and five towards the FHO side when measured from the tuber sacrale with a Myrin goniometer. No differences regarding stance time, swing time, or peak pressure between the FHO and non-FHO limb were seen in trot (p = 0.70, p = 0.26, and p = 0.91, respectively). Over the long term, the FHO limb has muscle atrophy, decreased coxofemoral extension, and decreased static weight bearing. However, this does not seem to affect the trot of the dogs. Dog owners considered the outcome of surgery to be good or excellent.
Differential Gene Expression Associated with Soybean Oil Level in the Diet of Pigs
Authors: Fanalli Simara Larissa , da Silva Bruna Pereira Martins , Gomes Julia Dezen , de Almeida Vivian Vezzoni , Freitas Felipe André Oliveira , Moreira Gabriel Costa Monteiro , Silva-Vignato Bárbara , Afonso Juliana , Reecy James , Koltes James , Koltes Dawn , de Almeida Regitano Luciana Correia , Garrick Dorian John , de Carvalho Balieiro Júlio Cesar , Meira Ariana Nascimento , Freitas Luciana , Coutinho Luiz Lehmann , Fukumasu Heidge , Mourão Gerson Barreto , de Alencar Severino Matias , Luchiari Filho Albino , Cesar Aline Silva Mello
Published: June 2022

The aim of this study was to identify the differentially expressed genes (DEG) from the skeletal muscle and liver samples of animal models for metabolic diseases in humans. To perform the study, the fatty acid (FA) profile and RNA sequencing (RNA-Seq) data of 35 samples of liver tissue (SOY1.5, n = 17 and SOY3.0, n = 18) and 36 samples of skeletal muscle (SOY1.5, n = 18 and SOY3.0, n = 18) of Large White pigs were analyzed. The FA profile of the tissues was modified by the diet, mainly those related to monounsaturated (MUFA) and polyunsaturated (PUFA) FA. The skeletal muscle transcriptome analysis revealed 45 DEG (FDR 10%), and the functional enrichment analysis identified network maps related to inflammation, immune processes, and pathways associated with oxidative stress, type 2 diabetes, and metabolic dysfunction. For the liver tissue, the transcriptome profile analysis revealed 281 DEG, which participate in network maps related to neurodegenerative diseases. With this nutrigenomics study, we verified that different levels of soybean oil in the pig diet, an animal model for metabolic diseases in humans, affected the transcriptome profile of skeletal muscle and liver tissue. These findings may help to better understand the biological mechanisms that can be modulated by the diet.
Lafora Disease and Alpha-Synucleinopathy in Two Adult Free-Ranging Moose (Alces alces) Presenting with Signs of Blindness and Circling
Authors: Ravi Madhu , Lacson Atilano , Pybus Margo , Ball Mark C.
Published: June 2022

Lafora disease is an autosomal recessive glycogen-storage disorder resulting from an accumulation of toxic polyglucosan bodies (PGBs) in the central nervous system, which causes behavioral and neurologic symptoms in humans and other animals. In this case study, brains collected from two young adult free-ranging moose (Alces alces) cows that were seemingly blind and found walking in circles were examined by light and electron microscopy. Microscopic analysis of the hippocampus of the brain revealed inclusion bodies resembling PGBs in the neuronal perikaryon, neuronal processes, and neuropil. These round inclusions measuring up to 30 microns in diameter were predominantly confined to the hippocampus region of the brain in both animals. The inclusions tested α-synuclein-negative by immunohistochemistry, α-synuclein-positive with PAS, GMS, and Bielschowsky’s staining; and diastase-resistant with central basophilic cores and faintly radiating peripheral lines. Ultrastructural examination of the affected areas of the hippocampus showed non-membrane-bound aggregates of asymmetrically branching filaments that bifurcated regularly, consistent with PGBs in both animals. Additionally, α-synuclein immunopositivity was noted in the different regions of the hippocampus with accumulations of small granules ultrastructurally distinct from PGBs and morphologically compatible with alpha-synucleinopathy (Lewy body). The apparent blindness found in these moose could be related to an injury associated with secondary bacterial invasion; however, an accumulation of neurotoxicants (PGBs and α-synuclein) in retinal ganglions cells could also be the cause. This is the first report demonstrating Lafora disease with concurrent alpha-synucleinopathy (Lewy body neuropathy) in a non-domesticated animal.
Effects of Selection on Breed Contribution in the Caballo de Deporte Español
Authors: Bartolomé Ester , Valera Mercedes , Fernández Jesús , Rodríguez-Ramilo Silvia Teresa
Published: June 2022

The equine breeding industry for sport’s performance has evolved into a fairly profitable economic activity. In particular, the Caballo de Deporte Español (CDE) is bred for different disciplines with a special focus on Show Jumping. The main aim of this study was to determine the effects of 15 years of selection and to find out whether it has been effective and adequate regarding the CDE main breeding objectives. The whole pedigree of 19,045 horses registered as CDE was used, comprising 47,884 animals (18,799 males and 29,085 females). An analysis performed to check for the pedigree completeness level yielded a number of equivalent complete generations (t) equal to 1.95, an average generation interval (GI) of 10.87 years, mean inbreeding coefficient (F) of 0.32%, an average relatedness coefficient (AR) of 0.09% and an effective population size (Ne) of 204. For the analyses, animals were divided into fourteen breed groups. Additionally, in order to study the evolution of these breeds over time and their influence on CDE pedigree, five different periods were considered according to the year of birth of the animals. Performance data used in the analyses were the estimated breeding values (EBV) of the Show Jumping sport discipline of 12,197 horses in the CDE pedigree, available from the 2020 routine genetic evaluations of the CDE breeding program (starting in 2004). Dressage and Eventing EBV values were also assessed. Results showed values of F higher than expected under random mating; this pointed to some degree of inbred matings. With regard to the evolution of breeding values, we found that, in general, EBVs of offspring were higher than the EBVs of parents. Notwithstanding, there is still a need for improvement in population management and the coordination of the breeders to get higher responses but controlling the loss of genetic diversity in the CDE breed.