bluuebunny/crossref_metadata_2025 huggingface dataset:
Naringin Inhibits Macrophage Foam Cell Formation by Regulating Lipid Homeostasis and Metabolic Phenotype
Authors: Liu Yan , Tang Xiaohan , Yuan Hailong , Gao Rong
Published: April 2024

Imbalances in lipid uptake and efflux and inflammation are major contributors to foam cell formation, which is considered a therapeutic target to protect against atherosclerosis. Naringin, a citrus flavonoid abundant in citrus fruits, has been reported to exert an antiatherogenic function, but its pharmacological mechanism is unclear. Naringin treatment effectively inhibits foam cell formation in THP-1 and RAW264.7 macrophages. In this study, mechanically, naringin maintained lipid homeostasis within macrophages through downregulation of the key genes for lipid uptake (MSR1 and CD36) and the upregulation of ABCA1, ABCG1 and SR-B1, which are responsible for cholesterol efflux. Meanwhile, naringin significantly decreased the cholesterol synthesis-related genes and increased the genes involved in cholesterol metabolism. Subsequently, the results showed that ox-LDL-induced macrophage inflammatory responses were inhibited by naringin by reducing the proinflammatory cytokines IL-1β, IL-6 and TNF-α, and increasing the anti- inflammatory cytokine IL-10, which was further verified by the downregulation of pro-inflammatory and chemokine-related genes. Additionally, we found that naringin reprogrammed the metabolic phenotypes of macrophages by suppressing glycolysis and promoting lipid oxidation metabolism to restore macrophage phenotypes and functions. These results suggest that naringin is a potential drug for the treatment of AS as it inhibits macrophage foam cell formation by regulating metabolic phenotypes and inflammation.
Two-Month Consumption of Orange Juice Enriched with Vitamin D3 and Probiotics Decreases Body Weight, Insulin Resistance, Blood Lipids, and Arterial Blood Pressure in High-Cardiometabolic-Risk Patients on a Westernized Type Diet: Results from a Randomized Clinical Trial
Authors: Papakonstantinou Emilia , Zacharodimos Nikolaos , Georgiopoulos Georgios , Athanasaki Christina , Bothou Dionysia-Lydia , Tsitsou Sofia , Lympaki Foteini , Vitsou-Anastasiou Stamatia , Papadopoulou Olga S. , Delialis Dimitrios , Alexopoulos Evangelos C. , Petsiou Eleni , Keramida Kalliopi , Doulgeraki Agapi I. , Patsopoulou Ismini-Maria , Nychas George-John E. , Tassou Chrysoula C.
Published: April 2024

This study examined the effects of orange juice (OJ) supplemented with vitamin D3 (2000 IU) and probiotics (Lacticaseibacillus casei Shirota and Lacticaseibacillus rhamnosus GG, 108 cfu/mL) on cardiometabolic risk factors in overweight and obese adults following a Westernized-type diet. Fifty-three high-risk individuals were randomly assigned to one of two groups. Over 8 weeks, one group consumed a vitamin D3 and probiotic-enriched OJ and the other regular OJ (control). Diets remained unchanged and were documented through food diaries. Measures of metabolic and inflammatory markers and blood pressure were measured at the start and end of the study. Post-intervention, the enriched OJ group showed the following significant metabolic improvements (without changes in triglycerides, inflammation, or central blood pressure): reduced fasting insulin, peripheral blood pressure, body weight (−1.4 kg 95% CI: −2.4, −0.4), energy (−270 kcal 95% CI: −553.2, −13.7), macronutrient (dietary fat −238 kcal 95% CI: −11.9, −1.0; carbohydrates −155 kcal 95% CI: −282.4, −27.3; sugars −16.1 g 95% CI: −11.9, −1.0) intake, and better lipid profiles (total cholesterol −10.3 mg/dL 95% CI: −21.4, 0.9; LDL-C −7 mg/dL 95% CI: −13.5, −0.5). The enriched OJ led to weight loss, less energy/macronutrient consumption, improved lipid profiles, and increased insulin sensitivity after 8 weeks in those following a Westernized diet, thus indicating potential benefits for cardiometabolic risk. This study was a part of FunJuice-T2EDK-01922, which was funded by the EU Regional Development Fund and Greek National Resources.
Changes in Body Composition and Nutritional Periodization during the Training Macrocycle in Football—A Narrative Review
Authors: Staśkiewicz-Bartecka Wiktoria , Kardas Marek , Zydek Grzegorz , Zając Adam , Chycki Jakub
Published: April 2024

Nutrition periodization in football training is an important determinant of adaptation to cyclic training loads. Personalizing an athlete’s diet is crucial to ensure optimal performance and body composition, depending on the phase of training. The purpose of this review is to answer the question of how the body composition of football players changes over the training macrocycle and how dietary recommendations should be tailored to specific training periods. The review of scientific evidence was conducted based on the available literature, typing in phrases related to training and nutrition periodization using the PubMed and Google Scholar database methodology tools. A literature search resulted in the selection of 346 sources directly related to the topic of the study, and then those with the highest scientific value were selected. There is a need to adjust energy and nutrient intake according to the different training phases in a football player’s preparation cycle. During the preparatory phase, it is recommended to increase protein and energy intake to support anabolic processes and muscle mass development. During the competitive period, due to the intensity of matches and training, the importance of carbohydrates for glycogen replenishment and recovery is emphasized. The transition phase requires the regulation of caloric intake to prevent adverse changes in body composition. Hydration has been identified as a key element in each phase of training. Cooperation between coaches, nutritionists, and players is essential to optimize sports performance and rapid recovery, and the authors recommend continuous adaptation and nutritional optimization as an integral part of football training.
Assessment of Drug Activities against Giardia Using Hyperspectral Raman Microscopy
Authors: Manciu Felicia S. , Guerrero Jose , Pence Breanna C. , Martinez Lopez Lizbeth V. , Das Siddhartha
Published: April 2024

This study demonstrates the capability of Raman microscopy for detecting structural differences in Giardia cells exposed to different drugs and incubation times. While metronidazole (MTZ) visibly affects the cells by inducing extracellular vesicle releases of toxic iron intermediates and modified triple-bond moieties, oseltamivir (OSM) alters the phenylalanine and lipid structures. Modifications in the heme protein environment and the transformation of iron from ferric to ferrous observed for both drug treatments are more notable for MTZ. Different contents and amounts of vesicle excretion are detected for 24 h or 48 h with MTZ incubation. At a shorter drug exposure, releases of altered proteins, glycogen, and phospholipids dominate. Agglomerates of transformed iron complexes from heme proteins and multiple-bond moieties prevail at 48 h of treatment. No such vesicle releases are present in the case of OSM usage. Drug incorporations into the cells and their impact on the plasma membrane and the dynamics of lipid raft confirmed by confocal fluorescence microscopy reveal a more destructive extent by OSM, corroborating the Raman results. Raman microscopy provides a broader understanding of the multifaceted factors and mechanisms responsible for giardiasis treatment or drug resistance by enabling a label-free, simultaneous monitoring of structural changes at the cellular and molecular levels.
Detectable Virological Load and Associated Factors among People Living with HIV on Antiretroviral Treatment: A Retrospective Study
Authors: Congedo Pierpaolo , Sedile Raffaella , Guido Marcello , Banchelli Federico , Zizza Antonella
Published: April 2024

The complete and prolonged suppression of viral load is the primary objective of HAART in people living with HIV. Some people may experience therapeutic failure, while others may achieve virological suppression but are unable to maintain it, developing persistent or single detection of low-level viremia. This study aims to evaluate the determinants of a detectable viral load among patients on HAART to identify and address them promptly. In this retrospective study, all patients referring to the Infectious Disease Operative Unit of the Vito Fazzi Hospital in Lecce, Puglia, older than 18 years, receiving HAART for at least 12 months as of 30 June 2022, were included. For each patient, demographic characteristics such as age, sex, educational level, stable relationship, cohabitation, employment status, and information relating to habits and lifestyles such as physical activity, use of drugs, and substances or supplements for sport, abuse of alcohol, and smoking were collected. Degree of comorbidity was quantified according to the Charlson Comorbidity Index, and the presence of obesity and the COVID-19 infection was also considered. Univariable and multivariable logistic regression models were used to assess the association between patients’ characteristics and the outcome. In the multivariable logistic regression model, the odds were lower for the duration of therapy (OR: 0.96; p = 0.0397), prescriber’s perception of adherence to therapy (OR: 0.50; p < 0.0001), and Nadir CD4+ T-cell count (OR: 0.85; p = 0.0329), and higher for the presence of AIDS (OR: 1.89; p = 0.0423) and COVID-19 (OR: 2.31; p = 0.0182). Our findings support the early initiation of HAART to achieve virological suppression. Additionally, measures to improve adherence to therapy should be adopted to ensure better outcomes for patients.
The Role of Prevotella Species in Female Genital Tract Infections
Authors: George Sheridan D. , Van Gerwen Olivia T. , Dong Chaoling , Sousa Lúcia G. V. , Cerca Nuno , Elnaggar Jacob H. , Taylor Christopher M. , Muzny Christina A.
Published: April 2024

Female genital tract infections (FGTIs) include vaginal infections (e.g., bacterial vaginosis [BV]), endometritis, pelvic inflammatory disease [PID], and chorioamnionitis [amniotic fluid infection]. They commonly occur in women of reproductive age and are strongly associated with multiple adverse health outcomes including increased risk of HIV/sexually transmitted infection acquisition and transmission, infertility, and adverse birth outcomes such as preterm birth. These FGTIs are characterized by a disruption of the cervicovaginal microbiota which largely affects host immunity through the loss of protective, lactic acid-producing Lactobacillus spp. and the overgrowth of facultative and strict anaerobic bacteria. Prevotella species (spp.), anaerobic Gram-negative rods, are implicated in the pathogenesis of multiple bacterial FGTIs. Specifically, P. bivia, P. amnii, and P. timonensis have unique virulence factors in this setting, including resistance to antibiotics commonly used in treatment. Additionally, evidence suggests that the presence of Prevotella spp. in untreated BV cases can lead to infections of the upper female genital tract by ascension into the uterus. This narrative review aims to explore the most common Prevotella spp. in FGTIs, highlight their important role in the pathogenesis of FGTIs, and propose future research in this area.
Precision Estimation of Crop Coefficient for Maize Cultivation Using High-Resolution Satellite Imagery to Enhance Evapotranspiration Assessment in Agriculture
Authors: Nagy Attila , Kiss Nikolett Éva , Buday-Bódi Erika , Magyar Tamás , Cavazza Francesco , Gentile Salvatore Luca , Abdullah Haidi , Tamás János , Fehér Zsolt Zoltán
Published: April 2024

The estimation of crop evapotranspiration (ETc) is crucial for irrigation water management, especially in arid regions. This can be particularly relevant in the Po Valley (Italy), where arable lands suffer from drought damages on an annual basis, causing drastic crop yield losses. This study presents a novel approach for vegetation-based estimation of crop evapotranspiration (ETc) for maize. Three years of high-resolution multispectral satellite (Sentinel-2)-based Normalized Difference Vegetation Index (NDVI), Normalized Difference Water Index (NDWI), Normalized Difference Red Edge Index (NDRE), and Leaf Area Index (LAI) time series data were used to derive crop coefficients of maize in nine plots at the Acqua Campus experimental farm of Irrigation Consortium for the Emilia Romagna Canal (CER), Italy. Since certain vegetation indices (VIs) (such as NDVI) have an exponential nature compared to the other indices, both linear and power regression models were evaluated to estimate the crop coefficient (Kc). In the context of linear regression, the correlations between Food and Agriculture Organization (FAO)-based Kc and NDWI, NDRE, NDVI, and LAI-based Kc were 0.833, 0.870, 0.886, and 0.771, respectively. Strong correlation values in the case of power regression (NDWI: 0.876, NDRE: 0.872, NDVI: 0.888, LAI: 0.746) indicated an alternative approach to provide crop coefficients for the vegetation period. The VI-based ETc values were calculated using reference evapotranspiration (ET0) and VI-based Kc. The weather station data of CER were used to calculate ET0 based on Penman-Monteith estimation. Out of the Vis, NDWI and NDVI-based ETc performed the best both in the cases of linear (NDWI RMSE: 0.43 ± 0.12; NDVI RMSE: 0.43 ± 0.095) and power (NDWI RMSE: 0.44 ± 0.116; NDVI RMSE: 0.44 ± 0.103) approaches. The findings affirm the efficacy of the developed methodology in accurately assessing the evapotranspiration rate. Consequently, it offers a more refined temporal estimation of water requirements for maize cultivation in the region.
Alginate Oligosaccharides Alleviate Salt Stress in Rice Seedlings by Regulating Cell Wall Metabolism to Maintain Cell Wall Structure and Improve Lodging Resistance
Authors: Du Youwei , Zhao Huimin , Feng Naijie , Zheng Dianfeng , Khan Aaqil , Zhou Hang , Deng Peng , Wang Yaxing , Lu Xutong , Jiang Wenxin
Published: April 2024

Salt stress is one of the major abiotic stresses that damage the structure and composition of cell walls. Alginate oligosaccharides (AOS) have been advocated to significantly improve plant stress tolerance. The metabolic mechanism by which AOS induces salt tolerance in rice cell walls remains unclear. Here, we report the impact of AOS foliar application on the cell wall composition of rice seedlings using the salt-tolerant rice variety FL478 and the salt-sensitive variety IR29. Data revealed that salt stress decreased biomass, stem basal width, stem breaking strength, and lodging resistance; however, it increased cell wall thickness. In leaves, exogenous AOS up-regulated the expression level of OSCESA8, increased abscisic acid (ABA) and brassinosteroids (BR) content, and increased β-galacturonic activity, polygalacturonase activity, xylanase activity, laccase activity, biomass, and cellulose content. Moreover, AOS down-regulated the expression levels of OSMYB46 and OSIRX10 and decreased cell wall hemicellulose, pectin, and lignin content to maintain cell wall stability under salt stress. In stems, AOS increased phenylalamine ammonia-lyase and tyrosine ammonia-lyase activities, while decreasing cellulase, laccase, and β-glucanase activities. Furthermore, AOS improved the biomass and stem basal width and also enhanced the cellulose, pectin, and lignin content of the stem, As a result, increased resistance to stem breakage strength and alleviated salt stress-induced damage, thus enhancing the lodging resistance. Under salt stress, AOS regulates phytohormones and modifies cellulose, hemicellulose, lignin, and pectin metabolism to maintain cell wall structure and improve stem resistance to lodging. This study aims to alleviate salt stress damage to rice cell walls, enhance resistance to lodging, and improve salt tolerance in rice by exogenous application of AOS.
Zinc and Silicon Nano-Fertilizers Influence Ionomic and Metabolite Profiles in Maize to Overcome Salt Stress
Authors: Shoukat Abbas , Saqib Zulfiqar Ahmad , Akhtar Javaid , Aslam Zubair , Pitann Britta , Hossain Md. Sazzad , Mühling Karl Hermann
Published: April 2024

Salinity stress is a major factor affecting the nutritional and metabolic profiles of crops, thus hindering optimal yield and productivity. Recent advances in nanotechnology propose an avenue for the use of nano-fertilizers as a potential solution for better nutrient management and stress mitigation. This study aimed to evaluate the benefits of conventional and nano-fertilizers (nano-Zn/nano-Si) on maize and subcellular level changes in its ionomic and metabolic profiles under salt stress conditions. Zinc and silicon were applied both in conventional and nano-fertilizer-using farms under stress (100 mM NaCl) and normal conditions. Different ions, sugars, and organic acids (OAs) were determined using ion chromatography and inductively coupled plasma mass spectroscopy (ICP-MS). The results revealed significant improvements in different ions, sugars, OAs, and other metabolic profiles of maize. Nanoparticles boosted sugar metabolism, as evidenced by increased glucose, fructose, and sucrose concentrations, and improved nutrient uptake, indicated by higher nitrate, sulfate, and phosphate levels. Particularly, nano-fertilizers effectively limited Na accumulation under saline conditions and enhanced maize’s salt stress tolerance. Furthermore, nano-treatments optimized the potassium-to-sodium ratio, a critical factor in maintaining ionic homeostasis under stress conditions. With the growing threat of salinity stress on global food security, these findings highlight the urgent need for further development and implementation of effective solutions like the application of nano-fertilizers in mitigating the negative impact of salinity on plant growth and productivity. However, this controlled environment limits the direct applicability to field conditions and needs future research, particularly long-term field trials, to confirm such results of nano-fertilizers against salinity stress and their economic viability towards sustainable agriculture.
A Cluster of Peronospora parasitica 13-like (NBS-LRR) Genes Is Associated with Powdery Mildew (Erysiphe polygoni) Resistance in Mungbean (Vigna radiata)
Authors: Waengwan Pitsanupong , Laosatit Kularb , Lin Yun , Yimram Tarika , Yuan Xingxing , Chen Xin , Somta Prakit
Published: April 2024

Powdery mildew (PM) caused by Erysiphe polygoni is an important foliar disease in mungbean (Vigna radiata). A previous study showed that QTL qPMRUM5-2 is a major locus for PM resistance in mungbean accession RUM5 (highly resistant). Bioinformatics analysis revealed that flanking markers of the qPMRUM5-2 covered a region of 1.93 Mb. In this study, we conducted fine mapping for the qPMRUM5-2 using the F2 population of 1156 plants of the cross between Chai Nat 60 (CN60; highly susceptible) and RUM5. PM resistance evaluation was performed under field conditions using F2:3 lines grown in three different environments. QTL analyses consistently located the qPMRUM5-2 to a 0.09 cm interval on linkage group 6 between InDel markers VrLG6-InDel05 and VrLG6-InDel10, which corresponded to a 135.0 kb region on chromosome 8 containing nine predicted genes of which five were NBS-LRR-type genes Recognition of Peronospora parasitica 13-like protein (RPP13L). Whole-genome re-sequencing of RUM5 and CN60 showed polymorphisms in four RPP13L genes predictively cause substantial amino acid changes, rendering them important candidate genes for PM resistance. The InDel markers VrLG6-InDel05 and VrLG6-InDel10 flanking to the qPMRUM5-2 would be useful for marker-assisted breeding of PM resistance in the mungbean.
Biodegradation Study of Styrene–Butadiene Composites with Incorporated Arthrospira platensis Biomass
Authors: Bumbac Marius , Nicolescu Cristina Mihaela , Zaharescu Traian , Gurgu Ion Valentin , Bumbac Costel , Manea Elena Elisabeta , Ionescu Ioana Alexandra , Serban Bogdan-Catalin , Buiu Octavian , Dumitrescu Crinela
Published: April 2024

The preparation of polymer composites that incorporate material of a biogenic nature in the polymer matrices may lead to a reduction in fossil polymer consumption and a potentially higher biodegradability. Furthermore, microalgae biomass as biogenic filler has the advantage of fast growth and high tolerance to different types of culture media with higher production yields than those provided by the biomass of terrestrial crops. On the other hand, algal biomass can be a secondary product in wastewater treatment processes. For the present study, an SBS polymer composite (SBSC) containing 25% (w/w) copolymer SBS1 (linear copolymer: 30% styrene and 70% butadiene), 50% (w/w) copolymer SBS2 (linear copolymer: 40% styrene and 60% butadiene), and 25% (w/w) paraffin oil was prepared. Arthrospira platensis biomass (moisture content 6.0 ± 0.5%) was incorporated into the SBSC in 5, 10, 20, and 30% (w/w) ratios to obtain polymer composites with spirulina biomass. For the biodegradation studies, the ISO 14855-1:2012(E) standard was applied, with slight changes, as per the specificity of our experiments. The degradation of the studied materials was followed by quantitatively monitoring the CO2 resulting from the degradation process and captured by absorption in NaOH solution 0.5 mol/L. The structural and morphological changes induced by the industrial composting test on the materials were followed by physical–mechanical, FTIR, SEM, and DSC analysis. The obtained results were compared to create a picture of the material transformation during the composting period. Thus, the collected data indicate two biodegradation processes, of the polymer and the biomass, which take place at the same time at different rates, which influence each other. On the other hand, it is found that the material becomes less ordered, with a sponge-like morphology; the increase in the percentage of biomass leads to an advanced degree of degradation of the material. The FTIR analysis data suggest the possibility of the formation of peptide bonds between the aromatic nuclei in the styrene block and the molecular residues resulting from biomass biodegradation. It seems that in industrial composting conditions, the area of the polystyrene blocks from the SBS-based composite is preferentially transformed in the process.
Synthesis and Oxidative Degradation of Leucine-Based Poly(diacylhydrazine)
Authors: Wongwailikhit Kanda , Suwannakeeree Ratha , Kihara Nobuhiro
Published: April 2024

Diacylhydrazine is thermally and chemically stable, and it remains inert to oxygen even at high temperatures. However, it is rapidly oxidized by sodium hypochlorite, leading to its decomposition into carboxylic acid and nitrogen gas. In the synthesis of a novel poly(diacylhydrazine) as an oxidatively degradable polymer, L-leucine methyl ester is acylated by terephthaloyl chloride. Subsequent hydrazination yields a bishydrazide monomer. The oxidative coupling polymerization of this monomer produces poly(diacylhydrazine). The molecular structures of the products are confirmed by an 1H NMR analysis. A polymodal molecular weight distribution and a large polydispersity index are observed by GPC in all cases. A 10% weight loss temperature is noted at 286 °C in air by TGA. The obtained polymer is not oxidized by oxygen. No glass transition is observed below the decomposition temperature. Upon the treatment of the poly(diacylhydrazine) with sodium hypochlorite solution, decomposition occurs rapidly, resulting in monomeric carboxylic acid and nitrogen gas. The L-leucine-based poly(diacylhydrazine) serves as a novel on-demand degradable polymer with high levels of thermal and chemical stability during usage.
Preparation of a Polymeric Phosphoramide Flame-Retardant and Its Effect on the Flame-Retardant Properties of Epoxy Resin
Authors: Wang Hao , Wang Yinjie , Su Yan , Yu Chuang , Han Jia , Liu Jiping
Published: April 2024

The flammability of epoxy resins and knowing how to achieve curing are particularly important factors during use. A novel approach for enhancing the fire resistance and reducing the smoke emission of epoxy resin during the curing process is suggested, which involves the utilization of a three-source integrated polymerization intumescent flame-retardant. In this study, the synthesis of poly 4,4-diaminodiphenylsulfone spirocyclic pentaerythritol bisphosphonate (PCS) is achieved through using solution polymerization, utilizing 4,4′-diaminodiphenylsulfone (DDS) and spirocyclic pentaerythritol bisphosphorate disphosphoryl chloride (SPDPC) as initial components. Following that, the EP underwent the inclusion of PCS to examine its resistance to heat, its ability to prevent flames, its effectiveness in reducing smoke and its curing effect. Compared to the unmodified epoxy resin, the addition of PCS can not only cure the epoxy resin, but also decompose before the epoxy resin and has a good carbonization effect. With the addition of 7 wt.% PCS, the LOI value can achieve 31.2% and successfully pass the UL-94 test with a V-0 rating. Moreover, the cone calorimeter experiment demonstrated a noteworthy decline of 59.7% in the maximum heat release rate (pHRR), 63.7% in overall heat release (THR), and 42.3% in total smoke generation (TSP). Based on the examination of TG-FTIR and SEM findings, there is ample evidence to suggest that PCS, functioning as a phosphorus-nitrogen intumescent flame-retardant that combines three origins, has the potential to exhibit a favorable flame-retardant impact in both its gas and condensed phases.
Development and Study of Novel Ultrafiltration Membranes Based on Cellulose Acetate
Authors: Kuzminova Anna , Dmitrenko Mariia , Dubovenko Roman , Puzikova Margarita , Mikulan Anna , Korovina Alexandra , Koroleva Aleksandra , Selyutin Artem , Semenov Konstantin , Su Rongxin , Penkova Anastasia
Published: April 2024

Recently, increasing attention of researchers in the field of membrane technology has been paid to the development of membranes based on biopolymers. One of the well-proven polymers for the development of porous membranes is cellulose acetate (CA). This paper is devoted to the study of the influence of different parameters on ultrafiltration CA membrane formation and their transport properties, such as the variation in coagulation bath temperature, membrane shrinkage (post-treatment at 80 °C), introduction to casting CA solution of polymers (polyethylene glycol (PEG), polysulfone (PS), and Pluronic F127 (PL)) and carbon nanoparticles (SWCNTs, MWCNTs, GO, and C60). The structural and physicochemical properties of developed membranes were studied by scanning electron and atomic force microscopies, Fourier-transform infrared spectroscopy, X-ray photoelectron spectroscopy, and contact angle measurements. The transport properties of developed CA-based membranes were evaluated in ultrafiltration of bovine serum albumin (BSA), dextran 110 and PVP K-90. All developed membranes rejected 90% compounds with a molecular weight from ~270,000 g/mol. It was shown that the combination of modifications (addition of PEG, PS, PL, PS-PL, and 0.5 wt% C60) led to an increase in the fluxes and BSA rejection coefficients with slight decrease in the flux recovery ratio. These changes were due to an increased macrovoid number, formation of a more open porous structure and/or thinner top selective, and decreased surface roughness and hydrophobization during C60 modification of blend membranes. Optimal transport properties were found for CA-PEG+C60 (the highest water—394 L/(m2h) and BSA—212 L/(m2h) fluxes) and CA-PS+C60 (maximal rejection coefficient of BSA—59%) membranes.
Preparation and Structural Analysis of a Water-Soluble Aminated Lignin
Authors: Zheng Qi , Nong Guangzai , Li Ning
Published: April 2024

Lignin is insoluble in water, thereby limiting its use in the synthesis of adhesives. Therefore, in this study, an aminated lignin compound was prepared through a lignin amination reaction to increase the amount of raw lignin material that can be used in the synthesis of adhesives; moreover, structural analysis was conducted. The main result of this was the introduction of amino groups into phenolic hydroxyl groups in the hydrolyzing lignin from the raw lignin materials, thus generating the product of aminated lignin. The resulting particle sizes were about 100 nm, the average molecular weight was 57,627 g/mol, and the water solubility of the aminated lignin was about 0.45 g/100 mL. Therefore, the water solubility of raw lignin was greatly improved. The proposed reaction mechanism of phenolic hydroxyl groups and carboxylic acid groups in lignin is a reaction with ammonia molecules; thus, the successful introduction of amino groups generated the aminated lignin compounds. Hence, this article enriches the scientific theory of lignin reactions and provides a reference for the widespread application of raw lignin materials in the field of adhesives.
The Effect of Thermoplastic Elastomer and Fly Ash on the Properties of Polypropylene Composites with Long Glass Fibers
Authors: Teodorescu George Mihail , Vuluga Zina , Ion Rodica Mariana , Fistoș Toma , Ioniță Andreea , Slămnoiu-Teodorescu Sofia , Paceagiu Jenica , Nicolae Cristian Andi , Gabor Augusta Raluca , Ghiurea Marius
Published: April 2024

A cost-effective solution to the problems that the automotive industry is facing nowadays regarding regulations on emissions and fuel efficiency is to achieve weight reduction of automobile parts. Glass fiber-reinforced polymers are regularly used to manufacture various components, and some parts may also contain thermoplastic elastomers for toughness improvement. This work aimed to investigate the effect of styrene-(ethylene-co-butylene)-styrene triblock copolymer (E) and treated fly ash (C) on the morphological, thermal, and mechanical properties of long glass fiber (G)-reinforced polypropylene (PP). Results showed that the composites obtained through melt processing methods presented similar thermal stability and improved (nano)mechanical properties compared to 25–30 wt.% G-reinforced PP composites (PP-25G/PP-30G). Specifically, the impact strength and surface hardness were greatly improved. The addition of 20 wt.% E led to a 25–39% increase in impact strength and surface elasticity, while the addition of 6.5 wt.% C led to a 16% increase in surface hardness. The composite based on 25 wt.% G, 6.5 wt.% C, and 20 wt.% E presented the best-balanced properties (8–17% increase in impact strength, 38–41% increase in axial strain, and 35% increase in surface hardness) compared with PP-30G/PP-25G. Structural and morphological analysis confirmed the presence of a strong interaction between the components that make the composites. Based on these results, the PP–G–E–C composites could be presented as a viable material for automotive applications.
Facile Prepared MOF-OH-PAN Nanofiber for Separation Co(II) from Waste Batteries
Authors: Yin Cong , Luo Yang , Pan Ting , Ding Liting , Wang Chenghuang , Yuan Guoyuan , Duan Chongxiong
Published: April 2024

Recovering cobalt from waste batteries is crucial for resource recycling and environmental protection. Here, MOF-OH, a Zr-based MOF, was synthesized and merged into a polyacrylonitrile (PAN) matrix to create MOF-OH-PAN nanofibers (NFs). These NFs showed a high cobalt ion adsorption capacity of 33.1 mg/g, retaining over 90% of the capacity after six cycles. The adsorption mechanism involves Co(II) surface diffusion followed by strong bonding with functional groups. This technology enables efficient cobalt recovery from waste batteries, supporting reuse and reducing resource depletion and environmental pollution. The study provides insights into waste battery resource management, highlighting environmental and economic benefits and contributing to green resource recovery and circular economy initiatives.
Influence of Biogenic Material Content on the Biodegradability of Styrene-Butadiene Composites with Incorporated Chlorella vulgaris Biomass
Authors: Bumbac Marius , Nicolescu Cristina Mihaela , Zaharescu Traian , Bumbac Costel , Manea Elena Elisabeta , Ionescu Ioana Alexandra , Gurgu Ion Valentin , Serban Bogdan-Catalin , Buiu Octavian , Dumitrescu Crinela
Published: April 2024

Bio-fillers are intensively studied for advanced polymer composite circular design and production. In this context, the algal biomass may be considered an important and relatively low-cost resource, when harvested as a by-product from wastewater treatment plants. The biomass of the algal species Chlorella vulgaris is frequently used in this type of environmental process, and its macro constituents’ composition ranges from around 15–25% carbohydrates, 10–20% lipids, and 50–60% proteins. Poly (styrene-butadiene-styrene) (SBS) copolymers have a matrix composed of glassy polystyrene domains connected by flexible polybutadiene segments. Although the physical-mechanical properties of SBS copolymers recommend them for many industrial applications, they have the drawback of low biodegradability. This study aimed to assess the aerobic biodegradability of polymer composites by integrating biomass from Chlorella vulgaris at varying mass percentages of 5, 10, and 20% into SBS copolymer composites. Biodegradation tests were conducted under industrial composting conditions (58 °C and 50% relative humidity) for 180 days. The biodegradability of materials was evaluated by measuring the CO2 produced in each vessel during the study period. Potential correlations between the amount of carbon dioxide released and the percentage of biomass added to the polymer matrix were examined. Structural and morphological changes were assessed using Fourier Transform infrared spectroscopy (FTIR), thermal analysis (DSC), and scanning electron microscopy (SEM). Physical and chemical testing revealed a decrease in sample density after the industrial composting test, along with noticeable changes in melt flow index (MFI). The observed physical and chemical changes, coupled with FTIR, SEM, and DSC data, indicate increased cross-linking and higher porosity in biodegraded polymer structures with higher biomass content. This behavior is likely due to the formation of cross-linked connections between polymer chains and polypeptide chains resulting from protein degradation, enhancing connections between polystyrene units facilitated by peptide bonds with the benzene units of the styrene blocks within the polymer matrix.
Multiplex Detection of Seven Staphylococcal Enterotoxins Using Liquid Chromatography–Mass Spectrometry Combined with a Novel Capture Molecule
Authors: Lv Jing , Liu Tingting , Fang Xinyu , Han Songyang , Dong Lina , Li Jiaxin , Wang Jing , Wang Jinglin , Gao Shan , Kang Lin , Xin Wenwen
Published: April 2024

Food poisoning caused by Staphylococcal enterotoxins (SEs) is prevalent globally, making efficient detection of these toxins very important. Traditionally, liquid chromatography–mass spectrometry required immunosorbent enrichment by magnetic bead-coupled antibodies obtained by animal-specific immunization. However, this method is time-consuming and costly. In this study, two recombinant protein capture molecules were designed based on the principle of toxins binding to Major Histocompatibility Complex (MHCII) and T cell receptor (TCR) molecules. The two capture molecules are called MHCII and MHCII-D10. The design of the MHCII and TCR-D10 was achieved through searching for the binding site protein sequence of Staphylococcal enterotoxins in the relevant literature, and MHCII-D10 was to link MHCII sequence with TCR-D10 sequence using linker (G4S)3 linking peptide. These capture molecules were shown to effectively bind to seven types of toxins and to capture SEs in various matrices. The digestion time, ratio, and temperature were further optimized, reducing the overall digestion time to just 2 h. The specificity, linearity, sensitivity, precision (RSD%), and recovery of the two methods were verified by liquid chromatography–mass spectrometry. When the MHCII and MHCII-D10 captured the toxins, the limit of quantification (LOD) in the 1 × PBS, plasma, and milk matrices ranged from 1.5625 to 100 fmol/µL, with the recovery rate ranging from 18.4% to 96%. The design of these capture molecules eliminates the need for animal-specific immunization, simplifying the pre-detection process and avoiding ethical concerns. This development holds significant promise for clinical diagnosis and reference.
Numerical Multifield Coupling Model of Stress Evolution and Gas Migration: Application of Disaster Prediction and Mining Sustainability Development
Authors: Kong Xiangguo , Zhao Tianshuo , Cai Yuchu , He Di
Published: April 2024

At present, coal mining is gradually shifting towards deep areas, and coal mines under deep mining conditions are more prone to coal and gas outburst accidents. In this research, we aim to explain the causes and mechanisms of dynamic disasters, which are caused by the combined action of static load, gas, and dynamic load on tectonic regions in complex stress field environments. Through numerical simulation using COMSOL Multiphysics software, based on the geological conditions of a mine in Jilin Province, it was found that faults lead to abnormal stress in tectonic regions. The combined action of dynamic and static loads results in excessive stress, causing the fragmentation and displacement of the coal body, leading to coal mine disasters, thus disrupting sustainability. Additionally, the coal matrix gas entering fractures raises the gas pressure and leads to the accumulation of methane near earthquake sources. Dynamic loads accelerate gas desorption in coal and increase porosity and permeability, facilitating rapid gas migration. This influx of gas into the roadways exceeds safety limits. Then, based on these findings and on-site conditions, a set of sustainable measures for coal mines has been proposed. This research offers theoretical guidance for enhancing safety, stability, and sustainability in coal mining processes.
Spatio-Temporal Evolution of Ecological Resilience in Ecologically Fragile Areas and Its Influencing Factors: A Case Study of the Wuling Mountains Area, China
Authors: Wu Jilin , Yang Manhong , Zuo Jinyou , Yin Ningling , Yang Yimin , Xie Wenhai , Liu Shuiliang
Published: April 2024

The ecological environment of the Wuling Mountains region has been impacted by climate change and economic development, necessitating immediate reinforcement of ecological protection and restoration measures. The study utilized the normalized vegetation index (NDVI) as a proxy for ecological resilience. NDVI data from 2000 to 2020 were employed to compute the ecological resilience index of the Wuling Mountains area and to examine its spatial and temporal evolution as well as the factors influencing it. The findings indicate that: (1) The ecological resilience index increased in the Wuling Mountains area and Guizhou, Chongqing, and Hunan sub-areas but decreased in the Hubei sub-area. (2) The ecological resilience varies significantly in the Wuling Mountains area and the Guizhou, Hubei, and Hunan sub-regions, whereas it varies less in the Chongqing sub-region. (3) The primary elements influencing the ecological resilience capability of the Wuling Mountains area and its four sub-areas are climate conditions and socio-economic factors, respectively. The study can offer a scientific foundation for ecological conservation and restoration efforts in the Wuling Mountains area, as well as serve as a benchmark for measuring ecological resilience in other environmentally vulnerable regions.
Sustainability in Natural Grassland in the Brazilian Pampa Biome: Livestock Production with CO2 Absorption
Authors: Roberti Débora Regina , Mergen Alecsander , Gotuzzo Ricardo Acosta , Veeck Gustavo Pujol , Bremm Tiago , Marin Luciana , de Quadros Fernando Luiz Ferreira , Jacques Rodrigo Josemar Seminoti
Published: April 2024

The Brazilian Pampa biome has natural pastures that have been used for centuries for cattle grazing. This is considered a sustainable system because it combines the conservation of natural vegetation and high-quality meat production, protecting the biome from commercial agriculture’s advances. However, whether it is a source or a sink of carbon dioxide (CO2) has yet to be evaluated. Hence, this study aimed to quantify the net ecosystem exchange (NEE) of the CO2 of a natural pasture of the Pampa biome used for livestock production. The experimental area is located in a subtropical region of southern Brazil, where eddy covariance (EC) measurements were conducted from 2015 to 2021 in a rotational cattle grazing system. The seven months of the warm season (September to March) were characterized as CO2 absorbers, while the five months of the cold season (April to August) were CO2 emitters. Throughout the six years and with complete data, the ecosystem was an absorber of atmospheric CO2, with an average value of −207.6 g C m−2 year−1. However, the significant interannual variability in NEE was observed, with cumulative values ranging from −82.0 to −385.3 g C m−2 year−1. The results suggest the coupling of climatic conditions to pasture management can be the factor that modulated the NEE interannual variability. The cattle raising system on the natural pastures of the Pampa absorbs CO2, which is further evidence of its sustainability and need for conservation.
Estimating Market Power Exertion in the U.S. Beef Packing Industry: An Illustration of Data Aggregation Bias Using Simulated Data
Authors: Lee Jung Min , Chung Chanjin
Published: April 2024

This study investigates data aggregation bias in estimating market power in the U.S. beef packing industry using New Empirical Industrial Organization (NEIO) models and shows empirical procedures that can alleviate the bias. Unlike many earlier studies in estimating market power exertion, our study examines the data aggregation bias when market-level data are used in place of firm-level data and show how the bias could be reduced. We first derive data aggregation bias analytically, then empirically investigate the aggregation bias by estimating both firm and aggregate industry models. Because the firm-level data are not available, we use simulated data generated from the Monte Carlo simulation method. Hybrid models, combining limited firm-level data with aggregate data, are also estimated to illustrate how the aggregation bias could be reduced. Our results show that aggregate models with industry-level data tend to underestimate market power exertion in the U.S. beef packing industry, and the aggregation bias is statistically significant at the 1% level. Comparing results from hybrid models with firm-level estimates, we find that hybrid models reduce the bias but do not remove the aggregation bias significantly. The sensitivity analysis shows that market power estimate and aggregation bias are sensitive to functional forms.
Sustainability Integration in Philippine Higher Education Curricula: A Structural Equation Modeling Assessing Teacher Intention to Integrate
Authors: Duran Ma. Kathleen L , Mariñas Klint Allen
Published: April 2024

Incorporating sustainability principles into university and college course offerings is pivotal in molding future leaders and innovators. This study focuses on the Philippines, where higher education institutions (HEIs) increasingly embed sustainability into their academic and operational frameworks. This study aims to quantitatively assess the level of teacher intention to integrate sustainability into curricula in higher education. Utilizing the expanded Theory of Planned Behavior (TPB), it examines the relationships between sustainability knowledge, concern for sustainability, perceived behavioral control, subjective norms, attitude towards sustainability integration, and the intention to integrate sustainability. The methodology involves a cross-sectional study using a web-based survey disseminated through multiple platforms. The sample size was determined through a priori calculation and proportional stratified sampling, with 227 respondents. Utilizing Structural Equation Modeling (SEM) and the Theory of Planned Behavior (TPB), this study uncovers that educators’ attitudes notably shape intentions to integrate sustainability into curricula. While the direct effect of perceived behavioral control is limited, institutional support is deemed to be crucial. Additionally, sustainability concerns strongly influence educators’ intentions, emphasizing the necessity for environmental awareness. These findings inform policymaking and underscore the significance of fostering sustainable practices in higher education through institutional support and awareness initiatives. Finally, this study aims to enhance the effectiveness of sustainability education in the Philippines and contribute to global sustainability efforts.
Indigenous Land-Based Perspectives on Environmental Sustainability: Learning from the Khasis Indigenous Community in Bangladesh
Authors: Datta Ranjan , Singha Rajmoni , Hurlbert Margot
Published: April 2024

This research explores Indigenous land-based perspectives on environmental sustainability, centering on the Khasis Indigenous community in Bangladesh. With a critical connection to their land-based cultural heritage and environment, the Khasis community offers a distinctive perspective for examining environmental challenges. Emphasizing the traditional land-based knowledge and practices of the Khasis, as well as their insights on environmental challenges, this study employs a land-based theoretical framework. It sheds light on the adaptive strategies of Khasis Indigenous communities amidst shifting environmental conditions, illustrating how they integrate sustainable practices into daily life by drawing upon Indigenous land-based knowledge and practices to mitigate environmental risks. Additionally, the research scrutinizes the challenges faced by the Khasis in preserving their land-based knowledge, practices, and identity in the face of a global environmental crisis. The findings contribute to broader discussions on Indigenous land-based knowledge and practices and their significance in contemporary climate discourse. By amplifying the voices of the Khasis Indigenous community’s land-based knowledge and practices, this study advocates for the incorporation of Indigenous perspectives into climate policies and interventions. It underscores the necessity for a more inclusive and culturally sensitive approach to environmental sustainability, one that acknowledges and respects the resilience of Indigenous communities like the Khasis in Bangladesh.
Safety of Cyclists in Poland in the Context of European Road Traffic
Authors: Pyra Mariusz
Published: April 2024

The growing interest in cycling as a means of urban transport has led to an increased focus on cyclist safety as a key aspect of urban planning and transport policy. Simulation studies conducted by the International Transport Forum have demonstrated that reductions in CO2 and other pollutants can be achieved in the context of urban transport, thus realising the goals of decarbonising road transport. The spread of modal transport in cities is a potential reality within the next decade. Bicycles play a significant role in this context. This article presents an analysis of data on accidents involving cyclists. National and international data were analysed to identify the main risk factors. The aim of the paper is to analyse the risk to cyclists with an attempt to identify and map the five biggest risks to cyclists in urban traffic. The aim of the research is to raise awareness of cyclist safety issues and to identify directions for further action to reduce accidents and improve overall road safety. The results of the conducted analyses indicate that the risk for cyclists in road traffic (including urban traffic) has been on a noticeable downward trend over the past five to ten years. This trend allows for the mapping of the most significant types/risks in cycling in urban environments, thereby enabling the implementation of risk management strategies based on the method of risk mapping.