bluuebunny/crossref_metadata_2025 huggingface dataset:
Sestrin2 provides cerebral protection through activation of Nrf2 signaling in microglia following subarachnoid hemorrhage
Authors: Yang Youqing , Ding Han , Yang Chenxing , Wu Jie , Bao Youyuan , Lan Shihai , Zhou Lin , Zhou Lu , Liu Bangliang , Hong Tao , Wan Xichen , Wu Xiao
Published: January 2023

Subarachnoid hemorrhage (SAH) is a neurological emergency characterized by dysfunctional inflammatory response. However, no effective therapeutic options have been reported so far. Microglia polarization has been proposed to exert an essential role in modulating inflammatory response after SAH. Sestrin2 is a stress response protein. Growing evidence has reported that sestrin2 could inhibit M1 microglia and promote M2 microglia polarization. The current study investigated the effects of sestrin2 on microglia phenotype switching and the subsequent brain injury and sought to elucidate the underlying mechanism. We conducted an endovascular perforation SAH model in mice. It was found that sestrin2 was significantly increased after SAH and was mainly distributed in neurons and microglia. Exogenous recombinant human sestrin2 (rh-sestrin2) evidently alleviated inflammatory insults and oxidative stress, and improved neurofunction after SAH. Moreover, rh-sestrin2 increased M2-like microglia polarization and suppressed the number of M1-like microglia after SAH. The protection by rh-sestrin2 was correlated with the activation of Nrf2 signaling. Nrf2 inhibition by ML385 abated the cerebroprotective effects of rh-sestrin2 against SAH and further manifested M1 microglia polarization. In conclusion, promoting microglia polarization from the M1 to M2 phenotype and inducing Nrf2 signaling might be the major mechanism by which sestrin2 protects against SAH insults. Sestrin2 might be a new molecular target for treating SAH.
25-hydroxyvitamin D3 generates immunomodulatory plasticity in human periodontal ligament-derived mesenchymal stromal cells that is inflammatory context-dependent
Authors: Behm Christian , Blufstein Alice , Gahn Johannes , Moritz Andreas , Rausch-Fan Xiaohui , Andrukhov Oleh
Published: January 2023

Introduction

Human periodontal ligament-derived mesenchymal stromal cells (hPDL-MSCs) exhibit a tight bi-directional interaction with CD4+ T lymphocytes. The hPDL-MSCs’ immunomodulatory abilities are drastically enhanced by pro-inflammatory cytokines via boosting the expression of various immunomediators. 25-hydroxyvitamin D3 (25(OH)D3), the major metabolite of vitamin D3 in the blood, affects both hPDL-MSCs and CD4+ T lymphocytes, but its influence on their interaction is unknown.

Methods

Therefore, primary hPDL-MSCs were stimulated in vitro with tumor necrosis factor (TNF)-α a or interleukin (IL)-1β in the absence and presence of 25(OH)D3 followed by an indirect co-culture with phytohemagglutinin-activated CD4+ T lymphocytes. The CD4+ T lymphocyte proliferation, viability, and cytokine secretion were analyzed. Additionally, the expression of various immunomediators in hPDL-MSCs was investigated, and their implication was verified by using pharmacological inhibitors.

Results

25(OH)D3 significantly counteracted the suppressive effects of IL-1β-treated hPDL-MSCs on CD4+ T lymphocyte proliferation, whereas no effects were observed in the presence of TNF-α. Additionally, 25(OH)D3 significantly increased the percentage of viable CD4+ T lymphocytes via TNF-α- or IL-1β-treated hPDL-MSCs. It also caused a significant decrease in interferon-γ, IL-17A, and transforming growth factor-β productions, which were triggered by TNF-α-treated hPDL-MSCs. 25(OH)D3 significantly decreased the production of various immunomediators in hPDL-MSCs. Inhibition of two of them, prostaglandin E2 and indoleamine-2,3-dioxygenase-1, partially abolished some of the hPDL-MSCs-mediated effects of 25(OH)D3 on CD4+ T lymphocytes.

Conclusion

These data indicate that 25(OH)D3 influences the immunomodulatory activities of hPDL-MSCs. This modulatory potential seems to have high plasticity depending on the local cytokine conditions and may be involved in regulating periodontal tissue inflammatory processes.
TRAF6 controls T cell homeostasis by maintaining the equilibrium of MALT1 scaffolding and protease functions
Authors: O’Neill Thomas J. , Gewies Andreas , Seeholzer Thomas , Krappmann Daniel
Published: January 2023

MALT1 is a core component of the CARD11-BCL10-MALT1 (CBM) signalosome, in which it acts as a scaffold and a protease to bridge T cell receptor (TCR) ligation to immune activation. As a scaffold, MALT1 binds to TRAF6, and T cell-specific TRAF6 ablation or destruction of MALT1-TRAF6 interaction provokes activation of conventional T (Tconv) effector cells. In contrast, MALT1 protease activity controls the development and suppressive function of regulatory T (Treg) cells in a T cell-intrinsic manner. Thus, complete loss of TRAF6 or selective inactivation of MALT1 catalytic function in mice skews the immune system towards autoimmune inflammation, but distinct mechanisms are responsible for these immune disorders. Here we demonstrate that TRAF6 deletion or MALT1 paracaspase inactivation are highly interdependent in causing the distinct immune pathologies. We crossed mice with T cell-specific TRAF6 ablation (Traf6-ΔT) and mice with a mutation rendering the MALT1 paracaspase dead in T cells (Malt1 PD-T) to yield Traf6-ΔT;Malt1 PD-T double mutant mice. These mice reveal that the autoimmune inflammation caused by TRAF6-ablation relies strictly on the function of the MALT1 protease to drive the activation of Tconv cells. Vice versa, despite the complete loss of Treg cells in Traf6-ΔT;Malt1 PD-T double mutant mice, inactivation of the MALT1 protease is unable to cause autoinflammation, because the Tconv effector cells are not activated in the absence of TRAF6. Consequentially, combined MALT1 paracaspase inactivation and TRAF6 deficiency in T cells mirrors the immunodeficiency seen upon T cell-specific MALT1 ablation.
Pushing the envelope: Immune mechanism and application landscape of macrophage-activating lipopeptide-2
Authors: Liao Daoyong , Su Xiaoling , Wang Jingyun , Yu Jianwei , Luo Haodang , Tian Wei , Ye Zufeng , He Jun
Published: January 2023

Mycoplasma fermentans can cause respiratory diseases, arthritis, genitourinary tract infections, and chronic fatigue syndrome and have been linked to the development of the human immunodeficiency virus. Because mycoplasma lacks a cell wall, its outer membrane lipoproteins are one of the main factors that induce inflammation in the organism and contribute to disease development. Macrophage-activating lipopeptide-2 (MALP-2) modulates the inflammatory response of monocytes/macrophages in a bidirectional fashion, indirectly enhances the cytotoxicity of NK cells, promotes oxidative bursts in neutrophils, upregulates surface markers on lymphocytes, enhances antigen presentation on dendritic cells and induces immune inflammatory responses in sebocytes and mesenchymal cells. MALP-2 is a promising vaccine adjuvant for this application. It also promotes vascular healing and regeneration, accelerates wound and bone healing, suppresses tumors and metastasis, and reduces lung infections and inflammation. MALP-2 has a simple structure, is easy to synthesize, and has promising prospects for clinical application. Therefore, this paper reviews the mechanisms of MALP-2 activation in immune cells, focusing on the application of MALP-2 in animals/humans to provide a basis for the study of pathogenesis in Mycoplasma fermentans and the translation of MALP-2 into clinical applications.
Skin-associated adipocytes in skin barrier immunity: A mini-review
Authors: Guan Jingyan , Wu Congxiao , He Yunfan , Lu Feng
Published: January 2023

The skin contributes critically to health via its role as a barrier tissue against a multitude of external pathogens. The barrier function of the skin largely depends on the uppermost epidermal layer which is reinforced by skin barrier immunity. The integrity and effectiveness of skin barrier immunity strongly depends on the close interplay and communication between immune cells and the skin environment. Skin-associated adipocytes have been recognized to play a significant role in modulating skin immune responses and infection by secreting cytokines, adipokines, and antimicrobial peptides. This review summarizes the recent understanding of the interactions between skin-associated adipocytes and other skin cells in maintaining the integrity and effectiveness of skin barrier immunity.
Hallmarks of crustacean immune hemocytes at single-cell resolution
Authors: Xin Fan , Zhang Xiaobo
Published: January 2023

In invertebrates, hemocytes are the key factors in innate immunity. However, the types of invertebrate immune hemocytes are unclassified due to the limitation of morphological classification. To determine the immune hemocytes of crustaceans, the heterogeneity of hemocytes of shrimp Marsupenaeus japonicus and crayfish Procambarus clarkii, two representative crustacean species, were characterized in this study. The results of single-cell RNA sequencing indicated that shrimp and crayfish contained 11 and 12 types of hemocytes, respectively. Each of different types of hemocytes specifically expressed the potential marker genes. Based on the responses of shrimp and crayfish to the infection of white spot syndrome virus (WSSV) and the challenge of lipopolysaccharide (LPS), four types of immune hemocytes of crustaceans were classified, including semi-granular hemocytes involved in antimicrobial peptide production, granular hemocytes responsible for the production of antimicrobial peptides, hemocytes related to cell proliferation and hemocytes in immunity-activated state. Therefore, our study provided the first classification of crustacean hemocytes as well as of immune hemocytes of crustaceans at the single-cell resolution, which would be helpful to understand the innate immunity of invertebrates.
LRRC3B and its promoter hypomethylation status predicts response to anti-PD-1 based immunotherapy
Authors: Luo Linfeng , Fu Sha , Du Wei , He Li-na , Zhang Xuanye , Wang Yixing , Zhou Yixin , Hong Shaodong
Published: January 2023

Background

The leucine rich repeat containing 3B (LRRC3B) gene is a tumor suppressor gene involved in the anti-tumor immune microenvironment. Expression of LRRC3B and DNA methylation at the LRRC3B promoter region may serve as a useful marker to predict response to anti-PD-1 therapy. However, no studies have yet systematically explored the protective role of LRRC3B methylation in tumor progression and immunity.

Methods

Expression of LRRC3B of 33 cancer types in The Cancer Genome Atlas (TCGA) was downloaded from UCSC Xena (http://xena.ucsc.edu/). And, we evaluated the differential expression of LRRC3B according to tumor stage, overall survival, and characteristics of the tumor microenvironment. The immunotherapeutic cohorts included IMvigor21, GSE119144, and GSE72308 which were obtained from the Gene Expression Omnibus database. We conducted pearson correlation analysis of LRRC3B and tumor microenvironment (TME) in pan-cancer. Also, six immune cell types (B cells, CD8+ T cells, CD4+ T cells, macrophages, neutrophils, and dendritic cells) and tumor purity were analyzed using the Tumor IMmune Estimation Resource (TIMER1.0) (Tumor IMmune Estimation Resource (TIMER2.0). And, a “silencing score” model base on LRRC3B promoter methylation to predict overall survival (OS) by multivariate Cox regression analysis was constructed. Finally, the model was applied to predict anti-PD-1 therapy in non-small cell lung cancer (NSCLC) and breast cancer (BRCA).

Results

LRRC3B expression associated with less tumor invasion, less severe tumor stage, and decreased metastasis. The inactivation of LRRC3B promoted the enrichment of immuneosuppressive cells, including myeloid-derived suppressor cells (MDSCs), cancer-associated fibroblasts (CAFs), M2 subtype of tumor-associated macrophages (M2-TAMs), M1 subtype of tumor-associated macrophages (M1-TAMs), and regulatory T (Treg) cells. A high silencing score was significantly associated with immune inhibition, low expression of LRRC3B, poor patient survival, and activation of cancer-related pathways.

Conclusion

Our comprehensive analysis demonstrated the potential role of LRRC3B in the anti-tumor microenvironment, clinicopathological features of cancer, and disease prognosis. It suggested that LRRC3B methylation could be used as a powerful biomarker to predict immunotherapy responses in NSCLC and BRCA.
dsRNase1 contribution to dsRNA degradation activity in the Sf9 cells conditioned medium
Authors: Koo Jinmo , Palli Subba Reddy
Published: January 2023

RNA interference (RNAi) is inefficient in lepidopteran insects, including Spodoptera frugiperda. RNase activity in the lumen and hemocoel is known to contribute to low RNAi efficiency in these insects. Conditioned medium from Sf9 cells developed from ovaries of S. frugiperda shows high dsRNA degradation activity. But the enzymes responsible for this activity have not been identified. The nuclease genes that are highly expressed in Sf9 cells, REase, RNaseT2, and dsRNase1, were identified. Knockdown of dsRNase1 in Sf9 cells resulted in a reduction of dsRNA degradation activity in the Sf9 cells conditioned medium. Knockdown of dsRNase1 also increased RNAi efficiency in Sf9 cells. The results from these studies identified a major player in dsRNA degradation activity in the Sf9 cells conditioned medium. We also describe an efficient system that can be used to identify other genes responsible for dsRNA degradation and RNAi efficiency in Sf9 cells.
A hyperspectral image reconstruction algorithm based on RGB image using multi-scale atrous residual convolution network
Authors: Hu Shaoxiang , Hou Rong , Ming Luo , Meifang Su , Chen Peng
Published: January 2023

Hyperspectral images are a valuable tool for remotely sensing important characteristics of a variety of landscapes, including water quality and the status of marine disasters. However, hyperspectral data are rare or expensive to obtain, which has spurred interest in low-cost, fast methods for reconstructing hyperspectral data from much more common RGB images. We designed a novel algorithm to achieve this goal using multi-scale atrous convolution residual network (MACRN). The algorithm includes three parts: low-level feature extraction, high-level feature extraction, and feature transformation. The high-level feature extraction module is composed of cascading multi-scale atrous convolution residual blocks (ACRB). It stacks multiple modules to form a depth network for extracting high-level features from the RGB image used as an input. The algorithm uses jump connection for residual learning, and the final high-level feature combines the output of the low-level feature extraction module and the output of the cascaded atrous convolution residual block element by element, so as to prevent gradient dispersion and gradient explosion in the deep network. Without adding too many parameters, the model can extract multi-scale features under different receptive fields, make better use of the spatial information in RGB images, and enrich the contextual information. As a proof of concept, we ran an experiment using the algorithm to reconstruct hyperspectral Sentinel-2 satellite data from the northern coast of Australia. The algorithm achieves hyperspectral spectral reconstruction in 443nm-2190nm band with less computational cost, and the results are stable. On the Realworld dataset, the reconstruction error MARE index is less than 0.0645, and the reconstruction time is less than 9.24S. Therefore, in the near infrared band, MACRN reconstruction accuracy is significantly better than other spectral reconstruction algorithms. MACRN hyperspectral reconstruction algorithm has the characteristics of low reconstruction cost and high reconstruction accuracy, and its advantages in ocean spectral reconstruction are more obvious.
Interannual variation of coastal upwelling around Hainan Island
Authors: Zhu Junying , Zhou Quanyi , Zhou Qianqing , Geng Xinxing , Shi Jie , Guo Xinyu , Yu Yang , Yang Ziwei , Fan Renfu
Published: January 2023

Coastal upwelling around Hainan Island has a significant impact on the physical environment, ecological environment, and fishery resources of the surrounding waters. Based on the satellite-observed sea surface temperature (SST) from 2003 to 2021, this study analyzed the interannual variations of SST around Hainan Island and the influence of the sea surface forcing on them, with special attention to the upwelling regions in Qiongdong and Qiongxi. The results show that satellite-observed SST can identify three upwelling regions located in the west (WS), east (ES), and northeast (NES) sides of the Hainan Island. The SSTs in the WS, ES, and NES showed warming trends of 0.045 °C/y, 0.010 °C/y, and 0.036 °C/y, respectively. The range of low-temperature water around Hainan Island has gradually shrunk over the past two decades, and this shrinkage trend was more prominent in the WS upwelling region than in the ES and NES upwelling regions. Both the EOF analysis and the SST difference between upwelling regions and non-upwelling regions suggests that the upwelling becomes stronger after 2013. However, it seems not to compete with the overall SST warming in the northwest area of the South China Sea. The interannual SST variations in the ES upwelling region were affected by the local wind stress curl in summer. Except for the wind stress curl, the interannual SST variations in NES upwelling are influenced by the stratification associated with local precipitation and wind direction, especially in 2018. In addition, the interannual SST variation in the WS upwelling region is closely related to the bottom cold water in the central Gulf of Tonkin, which was preserved from the previous winter and is influenced by winter atmospheric conditions. These results are helpful for understanding the variations in SST and ecosystem in the upwelling regions around Hainan Island.
Estimating and scaling-up biomass and abundance of epi- and infaunal bivalves in a Swedish archipelago region: Implications for ecological functions and ecosystem services
Authors: Greeve Youk , Bergström Per , Strand Åsa , Lindegarth Mats
Published: January 2023

Introduction

As suspension-feeders, bivalves play a key role in maintaining regulatory functions of coastal ecosystems, which are linked to important ecosystem services. The functions attributed to bivalves depend on the life habits of a species (epi- or infauna) and their abundance and biomass. To properly quantify and assess these functions, detailed information the distribution, abundance and biomass at the ecosystem scale is critical. Amongst others, this requires an understanding on how environmental conditions shape special patterns in distribution. In this study we investigate this fundamental information on the Swedish west coast, an area where this information is lacking.

Methods

A survey which was designed to representatively sample both epi- and infaunal bivalves from randomized locations in various habitat types was conducted. Specifically, abundance and biomass of all species were recorded in the intertidal (0-0.5 m) and the shallow subtidal zone (0.5-2 m). The sites were distributed over an offshore gradient and at two exposure levels. This sampling structure allowed to extrapolate the results to an ecosystem level though information on the areal extent of these habitats using GIS layers.

Results

It was found that even though there exist a great variability among sites, in general epifaunal bivalves outweigh infaunal bivalves approximately 3 to 1. In terms of abundance, the ratio is more or less reversed and infaunal species occur in greater numbers. Most bivalves were found at an intermediate level of exposure, but due to the areal extend of the sheltered inner-archipelago this was the most important habitat for bivalve abundance and biomass. It was also found that invasive epifaunal oyster Magallana gigas and the invasive infaunal clam Ensis leei both dominated their respective groups in terms of biomass.

Discussion

Though the survey was relatively small, these results serve as a valuable insight of the relative importance of epi- and infaunal bivalves in this region. This gives understanding on which species and habitats are particularly important for ecosystem functions and services related to bivalves. This also provide a starting baseline for attempts to quantify ecosystem services provided by certain species or groups of bivalves in the future.
Sediment provenances shift driven by sea level and Indian monsoon in the southern Bay of Bengal since the last glacial maximum
Authors: Liu Shengfa , Ye Wenxing , Zhang Hui , Cao Peng , Li Jingrui , Sun Xingquan , Li Xiaoyan , Fang Xisheng , Khokiattiwong Somkiat , Kornkanitnan Narumol , Shi Xuefa
Published: January 2023

The Tibetan Plateau uplift has induced the formation of the largest sediment source-sink system in the northeast Indian Ocean, which has become an ideal region for investigating land-sea interaction processes. However, many questions regarding sediment transport patterns and their controlling factors at different time scales remain unanswered. Therefore, in the present study, a gravity core named BoB-79, based on the southern Bay of Bengal (BoB) was selected to investigate sediment provenance shift and its corresponding mechanism to sedimentary environment change since the last glacial maximum (LGM). The clay mineral compositions are analyzed and the whole core sediments reveal a feature dominated by illite (~55%), followed by chlorite (~24%) and kaolinite (~17%), and the content of smectite (~4%) is the lowest. A trigonometric analysis of provenance discrimination of clay minerals showed that the Himalayas, together with the Indian Peninsula, represent the main sources of southern BoB sediments, and the last glacial period might have been controlled by the dominant Himalayan provenance, with an average contribution of approximately 90%. However, as a secondary source, the influence of the Indian Peninsula increased significantly during the Holocene, and its mean contribution was 24%, thus, indicating that it had a crucial effect on the evolution process of BoB. The sediment transportation pattern changed significantly from the LGM to the Holocene: in the last glacial period, the low sea level exposed the shelf area that caused the Ganges River connected with the largest submarine canyon in BoB named Swatch of No Ground (SoNG), and the Himalayan materials could be transported to the BoB directly under a strong turbidity current, thereby forming the deep sea deposition center with a sedimentation rate of 4.5 cm/kyr. Following Holocene, the sea level increased significantly, and the materials from multiple rivers around the BoB were directly imported into the continental shelf area. The intensive Indian summer monsoon dominated the transportation process of the terrestrial materials, thereby forming a deposition center in the shallow water area of the continental shelf northeast Indian Ocean; subsequently, the material flux relative to the input to the deep sea area decreased significantly, and the sedimentation rate in the southern BoB decreased to 1.7 cm/kyr.
Research on the influential characteristics of asymmetric wind fields on typhoon waves
Authors: Wu Yan , Dou Shentang , Fan Yaoshen , Yu Shoubing , Dai Weiqi
Published: January 2023

The East China Sea is an ocean region with frequent typhoons, typhoons are also the main reason for inducing typhoon waves. The complexity of typhoon waves is closely related to the complexity of typhoon wind field. In previous studies, symmetrical wind field models or superimposed wind field models were usually used to simulate typhoon waves. However, the actual wind fields are asymmetric, and the asymmetry is affected by many factors. Therefore, three wind field models are used to simulate the wind field of Typhoon Muifa that moved through the East China Sea. Moreover, the abovementioned wind field models are used to drive the third-generation wave model SWAN to simulate the wave field and wave spectrum of Typhoon Muifa. Studies show that the values generated by the asymmetric wind field model are most consistent with the actual measurement data. This is especially the case if the typhoon center is closer to the station. The accuracy of the typhoon waves simulated using the asymmetric wind field model is better than that of the other two wind field models. The asymmetric wind field can reflect the asymmetric characteristics of the typhoon well. The substantial wave heights on the right side of typhoon’s path are substantially higher than those on the left side of path. Additionally, the maximum wave spectral density and total energy of waves as simulated by the asymmetric wind field are both larger than those of the other two wind field models. Thus, the asymmetric wind field model is more suitable for the numerical simulation of typhoon waves in the East China Sea.
Mechanism of oceanic eddies in modulating the sea surface temperature response to a strong typhoon in the western North Pacific
Authors: Lin Sheng , Zhang Wen-Zhou , Wang Yuntao , Chai Fei
Published: January 2023

Eddies are ubiquitous mesoscale dynamics in oceans worldwide that prominently influence the upper ocean. Specifically, the presence of preexisting eddies can modulate oceanic responses to passing typhoons, for which the underlying mechanism requires further investigation. In 2013, Typhoon Soulik traveled over 2,000 km in the western North Pacific, encountering multiple eddies, offering a suitable opportunity for investigating the impact of eddies on typhoon-induced upper ocean responses. A coupled ocean-atmosphere model was developed for this event, and the simulations were validated with multiple observations. The surface cold wake after Typhoon Soulik was successfully reproduced by the coupled model, showing a multicore structure, which was due to the preexisting mesoscale cyclonic eddies along the typhoon path. An experimental case excluding eddies was also tested in the numerical model. The comparison between the control and experimental cases revealed that sea surface cooling was enhanced by eddies via the vertical mixing and horizontal advection of eddy-related currents. In particular, vertical mixing enhanced sea surface cooling beneath the typhoon center due to the stronger vertical stratification within the preexisting cyclonic oceanic eddies. The eddy-related current with a horizontal temperature gradient contributed to advection, enhancing sea surface cooling at the cooling center and leading to a rightward-shifted pattern of enhanced sea surface cooling. This study enriches the understanding of the complex upper ocean dynamics under the combined effects of typhoons and eddies.
Determinants of short birth interval among child-bearing age women in the Gedeb Hasasa district of the West Arsi zone, Ethiopia
Authors: Yosef Tewodros , Debela Degfachew , Shifera Nigusie
Published: January 2023

Background

Short birth intervals have been linked to higher rates of fetal loss, prenatal mortality, and poorer child survival. Therefore, for countries like Ethiopia that have a population policy intended at reducing fertility, understanding the level and factors influencing birth spacing is crucial in order to apply appropriate intervention. This study aimed to assess the prevalence and determinants of the short birth interval among child-bearing age women in the Gedeb Hasasa district of the West Arsi zone, Ethiopia.

Methods

A community-based cross-sectional study was conducted from 20 July to 20 August 2018. A multistage sampling method was used. Face-to-face interviews were conducted to gather data. The collected data were entered into Epi Data version 3.1 and later exported to SPSS version 21 for analysis. Logistic regression was used to identify factors associated with the short birth interval. The level of significance was declared at a p-value of <0.05.

Results

A total of 714 women participated, with a 98% response rate. The median birth interval length was 32 months. The prevalence of the short birth interval was 50.4%. After adjusting for confounding variables, being a rural resident [AOR = 2.50, 95% CI (1.52, 4.09)], having an illiterate husband [AOR = 4.14, 95% CI (2.15, 8.45)], breastfeeding duration for 7–12 months [AOR = 3.16, 95% CI (1.95, 5.13)] and 13–23 months [AOR = 2.45, 95% CI (1.52, 3.95)], sex of the prior child [AOR = 0.63, 95% CI (0.45, 0.88)], and previous child alive [AOR = 0.20, 95% CI (0.14, 0.96)] were the determinants of short birth interval.

Conclusion and recommendation

One in every two women practiced short birth intervals. The median birth interval duration was 32 months, which is below the minimum standard recommended by the WHO duration for the birth interval, which is 33 months. Short birth intervals were determined independently by residence, husband education, breastfeeding time, previous child’s sex, and previous child’s survival. Therefore, increasing women’s awareness of the ideal birth interval should be done through community health professionals and health developmental armies.
Removal of primary nutrient degraders reduces growth of soil microbial communities with genomic redundancy
Authors: McClure Ryan , Garcia Marci , Couvillion Sneha , Farris Yuliya , Hofmockel Kirsten S.
Published: January 2023

Introduction

Understanding how microorganisms within a soil community interact to support collective respiration and growth remains challenging. Here, we used a model substrate, chitin, and a synthetic Model Soil Consortium (MSC-2) to investigate how individual members of a microbial community contribute to decomposition and community growth. While MSC-2 can grow using chitin as the sole carbon source, we do not yet know how the growth kinetics or final biomass yields of MSC-2 vary when certain chitin degraders, or other important members, are absent.

Methods

To characterize specific roles within this synthetic community, we carried out experiments leaving out members of MSC-2 and measuring biomass yields and CO2 production. We chose two members to iteratively leave out (referred to by genus name): Streptomyces, as it is predicted via gene expression analysis to be a major chitin degrader in the community, and Rhodococcus as it is predicted via species co-abundance analysis to interact with several other members.

Results

Our results showed that when MSC-2 lacked Streptomyces, growth and respiration of the community was severely reduced. Removal of either Streptomyces or Rhodococcus led to major changes in abundance for several other species, pointing to a comprehensive shifting of the microbial community when important members are removed, as well as alterations in the metabolic profile, especially when Streptomyces was lacking. These results show that when keystone, chitin degrading members are removed, other members, even those with the potential to degrade chitin, do not fill the same metabolic niche to promote community growth. In addition, highly connected members may be removed with similar or even increased levels of growth and respiration.

Discussion

Our findings are critical to a better understanding of soil microbiology, specifically in how communities maintain activity when biotic or abiotic factors lead to changes in biodiversity in soil systems.
Candida albicans enriched in orthodontic derived white spot lesions and shaped focal supragingival bacteriome
Authors: Yang Hao , Ma Yansong , Xie Xianju , Wang Hongmei , Li Xiaowei , Fang Dongyu , Bai Yuxing
Published: January 2023

White spot lesions (WSLs) are common enamel infectious diseases in fixed orthodontic treatment, which might attribute to the dysbiosis of oral microbiome. However, the correlation of Candida albicans with oral bacteriome in WSLs still remains unrevealed. This study investigated the carriage of C. albicans and how it shaped the bacterial community in disease or healthy supragingival plaque, to explore the potential role of interkingdom interaction in orthodontic WSLs. In this study, 31 patients with WSLs (WSLs) and 23 healthy patients (Health) undergoing fixed orthodontic treatment were enrolled. The supragingival microbiota in both groups were determined using 16S rRNA gene sequencing. Colonization and abundance of C. albicans in the plaque were determined via culture-dependent and -independent methods. Among WSLs patients, the correlation of C. albicans and bacteriome was analyzed under QIIME2-based bioinformatics and Spearman’s correlation coefficient. The raw reads were deposited into the NCBI Sequence Read Archive (SRA) database (Accession Number: SRP404186). Significant differences in microbial diversity as well as composition were observed between WSLs and Health groups. Leptotrichia remarkably enriched in the WSLs group, while Neisseria and Cardiobacterium significantly enriched in the Health group. In addition, 45% of WSLs patients were C. albicans carriers but none in patients without WSLs. Among all WSLs patients, beta diversity and microbial composition were distinguished between C. albicans carriers and non-carriers. In C. albicans carriers, Corynebacterium matruchotii and Streptococcus mutans significantly enriched whereas Saccharibacteria_TM7_G-1 significantly depleted. The abundance of C. albicans was positively associated with bacteria such as Streptococcus mutans, while the negative correlation was detected between C. albicans and several bacteria such as Cardiobacterium hominis and Streptococcus sanguinis. Our study elucidated the distinguished supragingival plaque microbiome between orthodontic patients with and without WSLs. C. albicans frequently existed and enriched in orthodontic derived WSLs. The carriage of C. albicans shape plaque bacterial community in demineralized lesions and might play roles in WSLs pathogenesis.
Evaluation of the efficacy of the antimicrobial peptide HJH-3 in chickens infected with Salmonella Pullorum
Authors: Xu Yanzhao , Wang Qing , Dong Mengmeng , Song Huishuai , Hang Bolin , Sun Yawei , Zhang Huihui , Hu Jianhe
Published: January 2023

As a promising substitute for antibiotics, increasing attention has been given to the clinical application of antimicrobial peptides (AMPs). In this study, the mode of action of the HJH-3 against Salmonella Pullorum was investigated. The structure and properties of HJH-3 were examined in silico, and minimum inhibitory concentrations (MICs) were determined to evaluate its antimicrobial spectrum. The time-kill kinetics of HJH-3 was determined. The hemolytic activity of HJH-3 was determined by measuring the hemoglobin ultraviolet absorption value, and the cytotoxicity was determined using a CCK-8 kit. The protective effect of HJH-3 on chickens infected with S. Pullorum was evaluated in vivo. The results demonstrated that HJH-3 exhibited strong antibacterial activity against Gram-negative pathogens at MIC values of 1.5625–25 μg/mL and against Gram-positive pathogens at MIC values of 25–50 μg/mL. HJH-3 also showed activity against the Candida albicans (100 μg/mL) and Bacillus subtilis (6.25–12.5 μg/mL). HJH-3 at 100 μg/mL completely killed S. Pullorum after co-incubation for 6 h. Likewise, the hemolysis rate of CRBCs treated with 100 μg/mL HJH-3 (7.31%) was lower than that of CRBCs treated with 100 μg/mL pexiganan (40.43%). Although the hemolysis rate of CRBCs treated with 400 μg/mL HJH-3 was increased to 13.37%, it was much lower than that of 400 μg/mL pexiganan (57.27%). In regards to cytotoxicity, HJH-3 had almost no-effect on the CEF proliferation, pexiganan decreased CEFs proliferation from 56.93 to 31.00% when increasing the concentration from 50 to 200 μg/mL. In a chicken infection model, the results showed that the antibiotic prevention and HJH-3 prevention groups exhibited the best treatment effect, with the chickens being protected from the lethal dose of S. Pullorum, a decreased number of bacteria in the blood and spleen, and less pathological changes in intestinal segments. The prevention of infection by HJH-3 was similar to that by Ampicillin; the effect of treatment after infection was lower than that of treatment before infection, and the survival rate of infected chicks treated with HJH-3 was 70%, which was still higher than that of the infected chickens. These results suggest that HJH-3 has good clinical application potential and can be used as a substitute for antibiotics for the prevention and treatment of S. Pullorum infection.
Dual incorporation of non-canonical amino acids enables production of post-translationally modified selenoproteins
Authors: Morosky Pearl , Comyns Cody , Nunes Lance G. A. , Chung Christina Z. , Hoffmann Peter R. , Söll Dieter , Vargas-Rodriguez Oscar , Krahn Natalie
Published: January 2023

Post-translational modifications (PTMs) can occur on almost all amino acids in eukaryotes as a key mechanism for regulating protein function. The ability to study the role of these modifications in various biological processes requires techniques to modify proteins site-specifically. One strategy for this is genetic code expansion (GCE) in bacteria. The low frequency of post-translational modifications in bacteria makes it a preferred host to study whether the presence of a post-translational modification influences a protein’s function. Genetic code expansion employs orthogonal translation systems engineered to incorporate a modified amino acid at a designated protein position. Selenoproteins, proteins containing selenocysteine, are also known to be post-translationally modified. Selenoproteins have essential roles in oxidative stress, immune response, cell maintenance, and skeletal muscle regeneration. Their complicated biosynthesis mechanism has been a hurdle in our understanding of selenoprotein functions. As technologies for selenocysteine insertion have recently improved, we wanted to create a genetic system that would allow the study of post-translational modifications in selenoproteins. By combining genetic code expansion techniques and selenocysteine insertion technologies, we were able to recode stop codons for insertion of Nε-acetyl-l-lysine and selenocysteine, respectively, into multiple proteins. The specificity of these amino acids for their assigned position and the simplicity of reverting the modified amino acid via mutagenesis of the codon sequence demonstrates the capacity of this method to study selenoproteins and the role of their post-translational modifications. Moreover, the evidence that Sec insertion technology can be combined with genetic code expansion tools further expands the chemical biology applications.
HDAC4 in cancer: A multitasking platform to drive not only epigenetic modifications
Authors: Cuttini Emma , Goi Camilla , Pellarin Ester , Vida Riccardo , Brancolini Claudio
Published: January 2023

Controlling access to genomic information and maintaining its stability are key aspects of cell life. Histone acetylation is a reversible epigenetic modification that allows access to DNA and the assembly of protein complexes that regulate mainly transcription but also other activities. Enzymes known as histone deacetylases (HDACs) are involved in the removal of the acetyl-group or in some cases of small hydrophobic moieties from histones but also from the non-histone substrate. The main achievement of HDACs on histones is to repress transcription and promote the formation of more compact chromatin. There are 18 different HDACs encoded in the human genome. Here we will discuss HDAC4, a member of the class IIa family, and its possible contribution to cancer development.
Trans-UTPA: PSO and MADDPG based multi-UAVs trajectory planning algorithm for emergency communication
Authors: Li Jie , Cao Shuang , Liu Xianjie , Yu Ruiyun , Wang Xingwei
Published: January 2023

Communication infrastructure is damaged by disasters and it is difficult to support communication services in affected areas. UAVs play an important role in the emergency communication system. Due to the limited airborne energy of a UAV, it is a critical technical issue to effectively design flight routes to complete rescue missions. We fully consider the distribution of the rescue area, the type of mission, and the flight characteristics of the UAV. Firstly, according to the distribution of the crowd, the PSO algorithm is used to cluster the target-POI of the task area, and the neural collaborative filtering algorithm is used to prioritize the target-POI. Then we also design a Trans-UTPA algorithm. Based on MAPPO 's policy network and value function, we introduce transformer model to make Trans-UTPA's policy learning have no action space limitation and can be multi-task parallel, which improves the efficiency and generalization of sample processing. In a three-dimensional space, the UAV selects the emergency task to be performed (data acquisition and networking communication) based on strategic learning of state information (location information, energy consumption information, etc.) and action information (horizontal flight, ascent, and descent), and then designs the UAV flight path based on the maximization of the global value function. The experimental results show that the performance of the Trans-UTPA algorithm is further improved compared with the USCTP algorithm in terms of the success rate of each UAV reaching the target position, the number of collisions, and the average reward of the algorithm. Among them, the average reward of the algorithm exceeds the USCTP algorithm by 13%, and the number of collisions is reduced by 60%. Compared with the heuristic algorithm, it can cover more target-POIs, and has less energy consumption than the heuristic algorithm.
Role of plaque inflammation in symptomatic carotid stenosis
Authors: Zheng Yilong , Lim Mervyn Jun Rui , Tan Benjamin Yong-Qiang , Chan Bernard Poon Lap , Paliwal Prakash , Jonathan Ong Jia Yuan , Bharatendu Chandra , Chan Amanda Chee Yun , Yeo Leonard Leong Litt , Vijayan Joy , Hong Chiew S. , Chee Young Heng , Wong Lily Y. H. , Chen Jintao , Chong Victor Yao Feng , Dong Yanhong , Tan Chi Hsien , Sunny Sibi , Teoh Hock Luen , Sinha Arvind Kumar , Sharma Vijay Kumar
Published: January 2023

Objective

Prior studies have shown that plaque inflammation on FDG-PET and the symptomatic carotid atheroma inflammation lumen-stenosis (SCAIL) score were associated with recurrent ischemic events, but the findings have thus far not been widely validated. Therefore, we aimed to validate the findings of prior studies.

Methods

A single-center prospective cohort study that recruited patients with (1) recent TIA or ischemic stroke within the past 30 days, (2) ipsilateral carotid artery stenosis of ≥50%, and (3) were not considered for early carotid revascularization. The (1) maximum standardized uptake value (SUVmax) of the symptomatic carotid plaque, (2) the SCAIL score, and (3) stenosis severity of the symptomatic carotid artery were measured for all patients. The outcomes were (1) a 90-day ipsilateral ischemic stroke and (2) a 90-day ipsilateral symptomatic TIA or major adverse cardiovascular event (MACE).

Results

Among the 131 patients included in the study, the commonest cardiovascular risk factor was hypertension (95 patients, 72.5%), followed by diabetes mellitus (77 patients, 58.8%) and being a current smoker (64 patients, 48.9%). The median (IQR) duration between the index cerebral ischemic event and recruitment to the study was 1 (0, 2.5) days. The median (IQR) duration between the index cerebral ischemic event and FDG-PET was 5 (4, 7) days. A total of 14 (10.7%) patients had a 90-day stroke, and 41 (31.3%) patients had a 90-day TIA or MACE. On comparison of the predictive performances of the SCAIL score and SUVmax, SUVmax was found to be superior to the SCAIL score for predicting both 90-day ipsilateral ischemic stroke (AUC: SCAIL = 0.79, SUVmax = 0.92; p < 0.001; 95% CI = 0.072, 0.229) and 90-day TIA or MACE (AUC: SCAIL = 0.76, SUVmax = 0.84; p = 0.009; 95% CI = 0.020, 0.143).

Conclusion

Plaque inflammation as quantified on FDG-PET may serve as a reliable biomarker for risk stratification among patients with ECAD and recent TIA or ischemic stroke. Future studies should evaluate whether patients with significant plaque inflammation as quantified on FDG-PET benefit from carotid revascularization and/or anti-inflammatory therapy.
Genetic and environmental risk factors of acute infection-triggered encephalopathy
Authors: Mizuguchi Masashi , Shibata Akiko , Kasai Mariko , Hoshino Ai
Published: January 2023

Acute encephalopathy is a constellation of syndromes in which immune response, metabolism and neuronal excitation are affected in a variable fashion. Most of the syndromes are complex disorders, caused or aggravated by multiple, genetic and environmental risk factors. Environmental factors include pathogenic microorganisms of the antecedent infection such as influenza virus, human herpesvirus-6 and enterohemorrhagic Escherichia coli, and drugs such as non-steroidal anti-inflammatory drugs, valproate and theophylline. Genetic factors include mutations such as rare variants of the SCN1A and RANBP2 genes, and polymorphisms such as thermolabile CPT2 variants and HLA genotypes. By altering immune response, metabolism or neuronal excitation, these factors complicate the pathologic process. On the other hand, some of them could provide promising targets to prevent or treat acute encephalopathy.
An overview of the most threating diseases that affect worldwide citriculture: Main features, diagnose, and current control strategies
Authors: Barbieri Hellen Bertoletti , Fernandes Laura Soler , Pontes João Guilherme de Moraes , Pereira Alana Kelyene , Fill Taicia Pacheco
Published: January 2023

Citriculture has been one of the most important agricultural activities worldwide. Brazil is among the five major citrus producers in the world, together with China, the European Union (EU), the United States, and Mexico. Together, these five groups are responsible for about 50% of the global citrus fruit production and this industrial segment is responsible for generating direct and indirect jobs. However, several citric diseases such as citrus canker, huanglongbing, citrus variegated chlorosis, and citrus black spot have been associated with annual losses of tons of fruits and orange trees impacting the global economy for decades. Citrus canker is caused by the Gram-negative bacteria Xanthomonas citri subsp. citri. and is associated with symptoms such as the formation of chlorotic rings in the leaves, stems, and fruits. Huanglongbing (HLB) is mainly associated with the Gram-negative bacteria Candidatus Liberibacter spp. and its main symptoms are the appearance of yellowish branches and deformed small leaves with yellowish spots. Citrus variegated chlorosis (CVC) is associated with the bacterium Xylella fastidiosa and causes chlorotic spots with irregular edges on leaves and deformation of new leaves. Citrus black spot (CBS) is caused by the fungus Phyllosticta citricarpa and generates lesions on fruits and reddish-brown leaf lesions. Since citrus is important for global agriculture, the current review addresses the main features of these important diseases including their symptoms and transmission, as well as the diagnosis and control strategies that have been studied so far for application in the field.