bluuebunny/crossref_metadata_2025 huggingface dataset:
Heterobimetallic One-Dimensional Coordination Polymers MICuII (M = Li and K) Based on Ferromagnetically Coupled Di- and Tetracopper(II) Metallacyclophanes
Authors: T. da Cunha Tamyris , X. C. Oliveira Willian , F. Pedroso Emerson , Lloret Francesc , Julve Miguel , L. M. Pereira Cynthia
Published: August 2018

The synthesis, crystal structure and magnetic properties of the coordination polymers of formula [EDAP{Li6(H2O)8[(Cu2(μ-mpba)2)2(H2O)2]}]n (1) and [(EDAP)2{K(H2O)4[Cu2(μ-mpba)2(H2O)2]}Cl·2H2O]n (2), in which mpba = N,N′-1,3-phenylenebis(oxamate) and EDAP2+ = 1,1′-ethylenebis(4-aminopyridinium) are described. Both compounds have in common the presence of the [Cu2(mpba)2]4− tetraanionic unit which is a [3,3] metallacyclophane motif in which the copper(II) ions are five-coordinate in a distorted square pyramidal surrounding. The complex anion in 1 is dimerized through double out-of-plane copper to outer carboxylate-oxygen atoms resulting in the centrosymmetric tetracopper(II) fragment [Cu4(μ-mpba)4(H2O)2]8− which act as a ligand toward six hydrated lithium(I) cations leading to anionic ladder-like double chains whose charge is neutralized by the EDAP2+ cations. In the case of 2, each dicopper(II) entity acts as a ligand towards tetraquapotassium(I) units to afford anionic zig zag single chains of formula {K(H2O)4[Cu2(μ-mpba)2(H2O)2]}n3n− plus EDAP2+ cations and non-coordinate chloride anions. Cryomagnetic measurements on polycrystalline samples 1 and 2 show the occurrence of ferromagnetic interactions between the copper(II) ions across the –Namidate–(C–C–C)phenyl–Namidate– exchange pathway [J = +10.6 (1) and +8.22 cm−1 (2)] and antiferromagnetic ones through the double out-of-plane carboxylate-oxygen atoms [j = −0.68 cm−1 (1), the spin Hamiltonian being defined as H = − J ( S C u 1 · S C u 2 + S C u 2 i · S C u 1 i ) − j ( S C u 2 · S C u 2 i ) ].
Kinematics in the Information Age
Authors: Smeresky Brendon , Rizzo Alexa , Sands Timothy
Published: August 2018

Modern kinematics derives directly from developments in the 1700s, and in their current instantiation, have been adopted as standard realizations…or templates that seem unquestionable. For example, so-called aerospace sequences of rotations are ubiquitously accepted as the norm for aerospace applications, owing from a recent heritage in the space age of the late twentieth century. With the waning of the space-age as a driver for technology development, the information age has risen with the advent of digital computers, and this begs for re-evaluation of assumptions made in the former era. The new context of the digital computer defines the use of the term “information age” in the manuscript title and further highlights the novelty and originality of the research. The effects of selecting different Direction Cosine Matrices (DCM)-to-Euler Angle rotations on accuracy, step size, and computational time in modern digital computers will be simulated and analyzed. The experimental setup will include all twelve DCM rotations and also includes critical analysis of necessary computational step size. The results show that the rotations are classified into symmetric and non-symmetric rotations and that no one DCM rotation outperforms the others in all metrics used, yielding the potential for trade space analysis to select the best DCM for a specific instance. Novel illustrations include the fact that one of the ubiquitous sequences (the “313 sequence”) has degraded relative accuracy measured by mean and standard deviations of errors, but may be calculated faster than the other ubiquitous sequence (the “321 sequence”), while a lesser known “231 sequence” has comparable accuracy and calculation-time. Evaluation of the 231 sequence also illustrates the originality of the research. These novelties are applied to spacecraft attitude control in this manuscript, but equally apply to robotics, aircraft, and surface and subsurface vehicles.
Sesterterpenoid and Steroid Metabolites from a Deep-Water Alaska Sponge Inhibit Wnt/β-Catenin Signaling in Colon Cancer Cells
Authors: Park Hyun Bong , Tuan Nguyen Quoc , Oh Joonseok , Son Younglim , Hamann Mark T. , Stone Robert , Kelly Michelle , Oh Sangtaek , Na MinKyun
Published: August 2018

The Wnt/β-catenin signaling pathway is known to play critical roles in a wide range of cellular processes: cell proliferation, differentiation, migration and embryonic development. Importantly, dysregulation of this pathway is tightly associated with pathogenesis in most human cancers. Therefore, the Wnt/β-catenin pathway has emerged as a promising target in anticancer drug screening programs. In the present study, we have isolated three previously unreported metabolites from an undescribed sponge, a species of Monanchora (Order Poecilosclerida, Family Crambidae), closely related to the northeastern Pacific species Monanchora pulchra, collected from deep waters off the Aleutian Islands of Alaska. Through an assortment of NMR, MS, ECD, computational chemical shifts calculation, and DP4, chemical structures of these metabolites have been characterized as spirocyclic ring-containing sesterterpenoid (1) and cholestane-type steroidal analogues (2 and 3). These compounds exhibited the inhibition of β-catenin response transcription (CRT) through the promotion of β-catenin degradation, which was in part implicated in the antiproliferative activity against two CRT-positive colon cancer cell lines.
Antimicrobial and Antibiofilm Activities of Sulfated Polysaccharides from Marine Algae against Dental Plaque Bacteria
Authors: Jun Joon-Young , Jung Min-Jeong , Jeong In-Hak , Yamazaki Koji , Kawai Yuji , Kim Byoung-Mok
Published: August 2018

Dental plaque biofilms cause various dental diseases; therefore, inhibiting the growths of the dental plaque bacteria which produce biofilms can be a strategy for preventing dental disease. Certain sulfated polysaccharides from marine algae exert antimicrobial activities against human bacterial pathogens in addition to their physiological benefits. On the basis of these observations, the antimicrobial and antibiofilm activities of sulfated polysaccharides from different marine algae were evaluated against dental plaque bacteria. Among the sulfated polysaccharides, a fucoidan from Fucus vesiculosus showed notable antimicrobial activities against the selected dental plaque bacteria, including some foodborne pathogenic bacteria. The minimum inhibitory concentrations were of 125 to 1000 µg mL−1. Regarding the antibiofilm activity, the fucoidan at the concentrations of above 250 µg mL−1 completely suppressed the biofilm formations and planktonic cell growths of Streptococcus mutans and S. sobrinus. However, no eliminative effect on the completed biofilm was observed. The fucoidan consisted of almost fucose base polysaccharide containing approximately 14.0% sulfate content. The average molecular weight of the fucoidan was changed by heat treatment (121 °C for 15 min) and it affected the antimicrobial activity.
Evaluation of Atrial Electromechanical Delay to Predict Atrial Fibrillation in Hemodialysis Patients
Authors: Gunes Hakan , Sokmen Abdullah , Kaya Hakki , Gungor Ozkan , Kerkutluoglu Murat , Guzel Fatma Betul , Sokmen Gulizar
Published: August 2018

Background and objective: Prevalence of atrial fibrillation is higher in hemodialysis patients as compared to the general population. Atrial electromechanical delay is known as a significant predictor of atrial fibrillation. In this study, we aimed to reveal the relationship between atrial electromechanical delay and attacks of atrial fibrillation. Materials and methods: The study included 77 hemodialysis patients over 18 years of age giving written consent to participate in the study. The patients were divided into two groups based on the results of 24-h Holter Electrocardiogram (Holter ECG) as the ones having attacks of atrial fibrillation and the others without any attack of atrial fibrillation. Standard echocardiographic measurements were taken from all patients. Additionally, atrial conduction times were measured by tissue Doppler technique and atrial electromechanical delays were calculated. Results: Intra- and interatrial electromechanical delay were found as significantly lengthened in the group of patients with attacks of atrial fibrillation (p = 0.03 and p < 0.001 respectively). The optimal cut-off time for interatrial electromechanical delay to predict atrial fibrillation was >21 ms with a specificity of 79.3% and a sensitivity of 73.7% (area under the curve 0.820; 95% confidence interval (CI), 0.716–0.898). In the multivariate logistic regression model, interatrial electromechanical delay (odds ratio = 1.230; 95% CI, 1.104–1.370; p < 0.001) and hypertension (odds ratio = 4.525; 95% CI, 1.042–19.651; p = 0.044) were also associated with atrial fibrillation after adjustment for variables found to be statistically significant in univariate analysis and correlated with interatrial electromechanical delay. Conclusions: Interatrial electromechanical delay is independently related with the attacks of atrial fibrillation detected on Holter ECG records in hemodialysis patients.
Flavonoids and Other Phenolic Compounds from Medicinal Plants for Pharmaceutical and Medical Aspects: An Overview
Authors: Tungmunnithum Duangjai , Thongboonyou Areeya , Pholboon Apinan , Yangsabai Aujana
Published: August 2018

Phenolic compounds as well as flavonoids are well-known as antioxidant and many other important bioactive agents that have long been interested due to their benefits for human health, curing and preventing many diseases. This review attempts to demonstrate an overview of flavonoids and other phenolic compounds as the interesting alternative sources for pharmaceutical and medicinal applications. The examples of these phytochemicals from several medicinal plants are also illustrated, and their potential applications in pharmaceutical and medical aspects, especially for health promoting e.g., antioxidant effects, antibacterial effect, anti-cancer effect, cardioprotective effects, immune system promoting and anti-inflammatory effects, skin protective effect from UV radiation and so forth are highlighted.
Brazed Joint Interface Bonding Strength of AR500 Steel and AA7075 Aluminium Alloy
Authors: Muhamed Mohd Najib , Omar Mohd Zaidi , Abdullah Shahrum , Sajuri Zainuddin , Wan Zamri Wan Fathul Hakim , Abdullah Mohd Faizal
Published: August 2018

The joining of aluminium alloys to steels has been extensively studied, especially in the automotive sector. However, aluminium alloys are known to be difficult to join with steels when methods involving fusion welding are used because of the hot cracking problem. Hence, a high-strength joint between these dissimilar metals would be of benefit especially in reducing the weight of products. In this work, the torch-brazing method was applied to join AR500 steel with AA7075 aluminium alloy using Al–Si–Zn base filler metal at various flame times. The effects of the brazing work on the intermetallic phase formation and the mechanical strength of the joints were investigated. In this work, the maximum shear load obtained was 6460 N and the presence of the intermetallic phases had reduced the shear strength of the brazed joints. However, the torch-brazing process using Al–Si–Zn filler metal had successfully facilitated the joining of these dissimilar metals.
Opportunities and Challenges for Single-Unit Recordings from Enteric Neurons in Awake Animals
Authors: Barth Bradley , Huang Hsin-I , Hammer Gianna , Shen Xiling
Published: August 2018

Advanced electrode designs have made single-unit neural recordings commonplace in modern neuroscience research. However, single-unit resolution remains out of reach for the intrinsic neurons of the gastrointestinal system. Single-unit recordings of the enteric (gut) nervous system have been conducted in anesthetized animal models and excised tissue, but there is a large physiological gap between awake and anesthetized animals, particularly for the enteric nervous system. Here, we describe the opportunity for advancing enteric neuroscience offered by single-unit recording capabilities in awake animals. We highlight the primary challenges to microelectrodes in the gastrointestinal system including structural, physiological, and signal quality challenges, and we provide design criteria recommendations for enteric microelectrodes.
Microbial Community and Potential Pathogen Shifts Along an Ornamental Fish Supply Chain
Authors: Amaral-Zettler Linda A. , Schmidt Victor , Smith Katherine F.
Published: August 2018

There is broad interest in disease spread through the pet trade, but empirical research on hosts and pathogens in transit along actual trade routes is notably absent. Using next-generation DNA sequencing, and partnering with the ornamental fish industry, we tracked shifts in microbial community and potential pathogen structure associated with Sailfin Tang (Zebrasoma desjardinii) along the United States (U.S.) leg of an international supply chain. We observed striking changes in microbial diversity and composition of potential pathogens, including increased dominance of vibrios of fishes in transit. Our pilot findings suggest that high investment in fishes early in the supply chain may not matter to their long-term health depending on end destination conditions.
Effects of Xanthate on Flotation Kinetics of Chalcopyrite and Talc
Authors: Bai Limei , Liu Jie , Han Yuexin , Jiang Kai , Zhao Wenqing
Published: August 2018

This paper investigated the effects of using or not using potassium butyl xanthate (PBX) as a collector on the flotation kinetics of talc and chalcopyrite. By means of atomic force microscopy (AFM), Fourier-transform infrared spectroscopy (FTIR), a contact angle measuring instrument and particle size analyzer, the underlying causes behind the flotation rate changes of talc and chalcopyrite are analyzed. Experimental results showed that in collectorless flotation, the law of change in the flotation rate constant (k) of the two minerals over time is independent of pH, and k values of chalcopyrite are much smaller than those of talc. In the presence of PBX, the flotation speed of chalcopyrite greatly increases, and the k values of chalcopyrite are far larger than those of talc. This is mainly because the amount of xanthate adsorbed on the surface of chalcopyrite is large and the adsorption is in the form of chemisorption, while the adsorption of xanthate on the talcum surface is in very small amounts and in the form of physical adsorption. Simulation results indicated that the collectorless flotation of chalcopyrite conform to the classical first-order kinetics model and the Kelsall model, whereas that of talc only conform to the latter, which is due to the layered structure of talc. In the presence of the collector, talc flotation conforms to the two model, because talc has a higher floatability and particle morphology has less influence on the flotation rate.
Flotation Behavior of Diatomite and Albite Using Dodecylamine as a Collector
Authors: Gao Ying , Han Yuexin , Li Wenbo
Published: August 2018

The flotation behaviors of diatomite and albite using dodecylamine (DDA) as a collector were investigated and compared. The pure mineral flotation results indicate that the flotability difference between albite and diatomite is above 87% at pH 5.5 to 10.5. The recovery of albite improves with increasing DDA dosage at pH 5.5 to 10.5. In the same pH range, diatomite has weaker flotability than albite, particularly in alkaline pH pulp. Zeta potential measurements indicate that diatomite has a higher negative surface charge than albite at pH 7 to 12, DDA interacts strongly with albite and weakly with diatomite. Thus, DDA preferentially absorbs on albite surface rather than diatomite under alkaline conditions. Fourier transform infrared spectra (FTIR) indicate that the amount of DDA adsorbed to albite is greater than that adsorbed to diatomite, under the same conditions. The adsorption of DDA on the surface of diatomite is investigated by using atomic force microscopy (AFM) for the first time. The adsorption of the collector DDA on the surface of albite per unit area is greater than that on diatomite. This accounts for the lower recovery of diatomite than that of albite.
Rapid Evaluation of Spermidine from 12 Bean Cultivars by Direct Real-Time Mass Spectrometry Analysis
Authors: Wu Tao , Wu Xiaoyu , Yuan Xv , Wang Yi , Zhou Wenhua , Li Weili
Published: August 2018

The routine spermidine (SPD) detection method is time-consuming and laborious due to the lengthy chromatographic separation and/or tedious sample derivatization pretreatment. In this study, direct analysis in real-time ionization mode coupled with mass spectrometry (DART-MS) was developed to rapidly determine the SPD content of 12 bean cultivars. The results were compared in detail with those of the classical UHPLC-ESI-QTOF method. After conducting a series of optimizations, a simple sample extraction procedure employing 80% aqueous methanol, was followed by determination of sample extracts directly without any chromatographic separation or prior derivatization. The validated method showed excellent performance with low limits of detection (LOD of 0.025 mg·kg−1) and good recovery rates (102.79–148.44%). The investigation highlighted that the DART-MS method (~1.3 min per three samples) could be used as a high-throughput alternative to the classic UHPLC-ESI-QTOF method (~15 min per three samples).
Synthesis and Biological Evaluation of Novel Alkyl Amine Substituted Icariside II Derivatives as Potential Anticancer Agents
Authors: Wu Tong , Li Ting , Kang Ya-Nan , Liu Li , Wang Xi-Man , Lan Jin-Shuai , Ding Yue , Zhang Tong
Published: August 2018

A series of novel alkyl amine-substituted icariside II (ICA II) derivatives were synthesized by Mannich reactions at the 6-C position (compounds 4a–d) and changing the carbon chain length at the 7-OH position (compounds 7a–h), and their in vitro antitumor activity towards human breast cancer lines (MCF-7 and MDA-MB-231) and human hepatoma cell lines (HepG2 and HCCLM3-LUC) were evaluated by the MTT assay. Compared with ICA II, most of the twelve derivatives showed good micromole level activity and a preliminary structure-activity relationship (SAR) for the anticancer activity was obtained. Compound 7g showed the most potent inhibitory activity for the four cancer cell lines (13.28 μM for HCCLM3-LUC, 3.96 μM for HepG2, 2.44 μM for MCF-7 and 4.21 μM for MDA-MB-231), which was 2.94, 5.54, 12.56 and 7.72-fold stronger than that of ICA II. The preliminary SAR showed that the introduction of a alkyl amine substituent at 6-C was not favorable for the anticancer activity, while most of the 7-O-alkylamino derivatives exhibited good antitumor activity and the anticancer activity 7-O-alkylamino derivatives were influenced by the alkyl chain length and the different terminal amine substituents. Furthermore, the effects of compound 7g on apoptosis and cell cycle of MCF-7 cells were further investigated, which showed that compound 7g triggered apoptosis and arrested the cell cycle at the G0/G1 phase in MCF-7 cells. Our findings indicate that compound 7g may be a promising anticancer drug candidate lead.
Design, Synthesis, and Neuroprotective Effects of a Series of Pyrazolines against 6-Hydroxydopamine-Induced Oxidative Stress
Authors: Özdemir Ahmet , Sever Belgin , Altıntop Mehlika Dilek , Kaya Tilki Elif , Dikmen Miriş
Published: August 2018

Parkinson’s disease (PD) is a chronic, progressive, and age-related neurodegenerative disorder characterized by the loss of midbrain dopaminergic neurons caused by the accumulation of free radicals and oxidative stress. Based on the neuroprotective properties of 2-pyrazoline derivatives, in the current work, 1-(phenyl/4-substituted phenyl)-3-(2-furanyl/thienyl)-5-aryl-2-pyrazolines (3a–i, 4a–i) were synthesized via the cyclization of the chalcones (1, 2) with suitable phenylhydrazine hydrochloride derivatives. All these compounds were investigated for their neuroprotective effects using an in vitro 6-hydroxydopamine (6-OHDA)-induced neurotoxicity model of PD in the rat pheochromocytoma (PC-12) Adh cell line. In addition, some different pharmacokinetic parameters of all compounds were in silico predicted by the QikProp module of Schrödinger’s Maestro molecular modeling package. 4-Methylsulfonylphenyl substituted compounds 3h (20%) and 4h (23%) were determined as the most promising neuroprotective agents related to their inductive roles in cell viability when compared with the 6-OHDA-positive control group (43% and 42%, respectively). Moreover, in silico pharmacokinetic results indicated that all compounds were within the acceptable range intended for human use. According to both in vitro and in silico studies, compounds 3h and 4h draw attention as potential orally bioavailable therapeutic drug candidates against neurodegeneration in PD.
Development of a NIR Method for the In-Line Quantification of the Total Polyphenolic Content: A Study Applied on Ajuga genevensis L. Dry Extract Obtained in a Fluid Bed Process
Authors: Gavan Alexandru , Colobatiu Liora , Mocan Andrei , Toiu Anca , Tomuta Ioan
Published: August 2018

This study describes an innovative in-line near-infrared (NIR) process monitoring method for the quantification of the total polyphenolic content (TPC) of Ajuga genevensis dry extracts. The dry extract was obtained in a fluidized bed processor, by spraying and adsorbing a liquid extract onto an inert powder support. NIR spectra were recorded continuously during the extract’s spraying process. For the calibration of the in-line TPC quantification method, samples were collected during the entire process. The TPC of each sample was assessed spectroscopically, by applying a UV-Vis reference method. The obtained values were further used in order to develop a quality OPLS prediction model by correlating them with the corresponding NIR spectra. The final dry extract registered good flowability and compressibility properties, a concentration in active principles three times higher than the one of the liquid extract and an overall process yield of 85%. The average TPC’s recovery of the NIR in-line prediction method, compared with the reference UV-Vis one, was 98.7%, indicating a reliable monitoring method which provided accurate predictions of the TPC during the process, permitting a good process overview and enabling us to establish the process’s end point at the exact moment when the product reaches the desired TPC concentration.
Further Studies on the Pyrolytic Domino Cyclization of Stabilized Phosphonium Ylides Bearing an Ortho-Aminophenyl Group
Authors: Aitken R. Alan , Murray Lorna , Slawin Alexandra M. Z.
Published: August 2018

Four new, stabilized phosphonium ylides containing a 2-(benzyl(methyl)amino)phenyl group have been prepared and characterized and are found, upon pyrolysis under gas-phase flow conditions, to lose Ph3PO and benzyl radicals to afford new heterocyclic products resulting from domino cyclization of both C- and N-centered radicals. Most products arise from processes of the former type and have quinoline, phenanthridine, or ring-fused phenanthridine structures, while in one case, a process of the latter type leads to a benzocarbazole product. The X-ray structure of a 2-(methyl(tosyl)amino)phenyl ylide is also reported.
Evaluating Nanoshells and a Potent Biladiene Photosensitizer for Dual Photothermal and Photodynamic Therapy of Triple Negative Breast Cancer Cells
Authors: Riley Rachel , O’Sullivan Rachel , Potocny Andrea , Rosenthal Joel , Day Emily
Published: August 2018

Light-activated therapies are ideal for treating cancer because they are non-invasive and highly specific to the area of light application. Photothermal therapy (PTT) and photodynamic therapy (PDT) are two types of light-activated therapies that show great promise for treating solid tumors. In PTT, nanoparticles embedded within tumors emit heat in response to laser light that induces cancer cell death. In PDT, photosensitizers introduced to the diseased tissue transfer the absorbed light energy to nearby ground state molecular oxygen to produce singlet oxygen, which is a potent reactive oxygen species (ROS) that is toxic to cancer cells. Although PTT and PDT have been extensively evaluated as independent therapeutic strategies, they each face limitations that hinder their overall success. To overcome these limitations, we evaluated a dual PTT/PDT strategy for treatment of triple negative breast cancer (TNBC) cells mediated by a powerful combination of silica core/gold shell nanoshells (NSs) and palladium 10,10-dimethyl-5,15-bis(pentafluorophenyl)biladiene-based (Pd[DMBil1]-PEG750) photosensitizers (PSs), which enable PTT and PDT, respectively. We found that dual therapy works synergistically to induce more cell death than either therapy alone. Further, we determined that low doses of light can be applied in this approach to primarily induce apoptotic cell death, which is vastly preferred over necrotic cell death. Together, our results show that dual PTT/PDT using silica core/gold shell NSs and Pd[DMBil1]-PEG750 PSs is a comprehensive therapeutic strategy to non-invasively induce apoptotic cancer cell death.
Complex Three-Dimensional Co3O4 Nano-Raspberry: Highly Stable and Active Low-temperature CO Oxidation Catalyst
Authors: Fuchigami Teruaki , Kimata Ryosuke , Haneda Masaaki , Kakimoto Ken-ichi
Published: August 2018

Highly stable and active low-temperature CO oxidation catalysts without noble metals are desirable to achieve a sustainable society. While zero-dimensional to three-dimensional Co3O4 nanoparticles show high catalytic activity, simple-structured nanocrystals easily self-aggregate and become sintered during catalytic reaction. Thus, complex three-dimensional nanostructures with high stability are of considerable interest. However, the controlled synthesis of complex nanoscale shapes remains a great challenge as no synthesis theory has been established. In this study, 100 nm raspberry-shaped nanoparticles composed of 7–8 nm Co3O4 nanoparticles were synthesized by hydrothermally treating cobalt glycolate solution with sodium sulfate. Surface single nanometer-scale structures with large surface areas of 89 m2·g−1 and abundant oxygen vacancies were produced. The sulfate ions functioned as bridging ligands to promote self-assembly and suppress particle growth. The Co3O4 nano-raspberry was highly stable under catalytic tests at 350 °C and achieved nearly 100% CO conversion at room temperature. The addition of bridging ligands is an effective method to control the formation of complex but ordered three-dimensional nanostructures that possessed extreme thermal and chemical stability and exhibited high performance.
Actively Tunable Terahertz Switches Based on Subwavelength Graphene Waveguide
Authors: Guo Zhongyi , Nie Xiaoru , Shen Fei , Zhou Hongping , Zhou Qingfeng , Gao Jun , Guo Kai
Published: August 2018

As a new field of optical communication technology, on-chip graphene devices are of great interest due to their active tunability and subwavelength scale. In this paper, we systematically investigate optical switches at frequency of 30 THz, including Y-branch (1 × 2), X-branch (2 × 2), single-input three-output (1 × 3), two-input three-output (2 × 3), and two-input four-output (2 × 4) switches. In these devices, a graphene monolayer is stacked on the top of a PMMA (poly methyl methacrylate methacrylic acid) dielectric layer. The optical response of graphene can be electrically manipulated; therefore, the state of each channel can be switched ON and OFF. Numerical simulations demonstrate that the transmission direction can be well manipulated in these devices. In addition, the proposed devices possess advantages of appropriate ON/OFF ratios, indicating the good performance of graphene in terahertz switching. These devices provide a new route toward terahertz optical switching.
TiO2 Nanotubes/Ag/MoS2 Meshy Photoelectrode with Excellent Photoelectrocatalytic Degradation Activity for Tetracycline Hydrochloride
Authors: Li Tingting , Wang Zhuhong , Liu Chaochao , Tang Chunmin , Wang Xinkai , Ding Gongsheng , Ding Yichun , Yang Lixia
Published: August 2018

A novel type of TiO2 nanotubes (NTs)/Ag/MoS2 meshy photoelectrode was fabricated with highly oriented TiO2 nanotube arrays grown from a Ti mesh supporting Ag nanoparticles and three-dimensional MoS2 nanosheets. In this structure, Ag nanoparticles act as bridges to connect MoS2 and TiO2 and pathways for electron transfer, ensuring the abundant production of active electrons, which are the source of •O2−. The TiO2 NTs/Ag/MoS2 mesh can be used as both photocatalyst and electrode, exhibiting enhanced photoelectrocatalytic efficiency in degrading tetracycline hydrochloride under visible light irradiation (λ ≥ 420 nm). Compared to unmodified TiO2 NTs, the improved photoelectrocatalytic activity of the TiO2 NTs/Ag/MoS2 arise from the formation of Z-scheme heterojunctions, which facilitate the efficient separation of photogenerated electron-hole pairs through the Schottky barriers at the interfaces of TiO2 NTs–Ag and Ag–MoS2.
Association of Anabolic Effect of Calcitriol with Osteoclast-Derived Wnt 10b Secretion
Authors: Lu Chien-Lin , Shyu Jia-Fwu , Wu Chia-Chao , Hung Chi-Feng , Liao Min-Tser , Liu Wen-Chih , Zheng Cai-Mei , Hou Yi-Chou , Lin Yuh-Feng , Lu Kuo-Cheng
Published: August 2018

Canonical Wnt (Wingless/Integrated) signaling is crucial in bone development and the Wnt ligand can promote osteoblast differentiation from mesenchymal progenitor cells. Calcitriol, an active vitamin D3, is used clinically for treatment of secondary hyperparathyroidism (SHPT) in chronic kidney disease (CKD) patients. The bone effects of calcitriol in SHPT remains uncertain. We hypothesized that calcitriol improves bone mass by suppressing osteoclast activity, and simultaneously promoting Wnt ligand secretion. We designed a cross-sectional study in maintenance hemodialysis patients to explore the effects of calcitriol on different bone turnover markers and specifically emphasized the Wnt 10b levels. Then, we explored the source of Wnt 10b secretion by using osteoclasts and osteoblasts treated with calcitriol in cell culture studies. Finally, we explored the effects of calcitriol on bone microarchitectures in CKD mice, using the 5/6 nephrectomy CKD animal model with analysis using micro-computed tomography. Calcitriol promoted the growth of both trabecular and cortical bones in the CKD mice. Wnt 10b and Procollagen 1 N-terminal Propeptide (P1NP) significantly increased, but Tartrate-resistant acid phosphatase 5b (Trap 5b) significantly decreased in the calcitriol-treated maintenance hemodialysis patients. Calcitriol enhanced Wnt 10b secretion from osteoclasts in a dose-dependent manner. Treatment of SHPT with calcitriol improved the bone anabolism by inhibiting osteoclasts and promoting osteoblasts that might be achieved by increasing the Wnt 10b level.
Non-Antibiotic Antimicrobial Catheter Lock Solutions in Patients on Home Parenteral Nutrition
Authors: Noelting Jessica , Jurewitsch Brian , Allard Johane
Published: August 2018

Patients on home parenteral nutrition (HPN) are dependent on central venous access for long-term sustenance, and catheter-related bloodstream infections (CRBSIs) are a major cause of morbidity and mortality in this patient population. As such, there is much interest in finding new methods for preventing CRBSIs in patients on HPN. As it is thought that these infections are preceded by microbial colonization of the catheter, one approach is to use antimicrobial catheter lock solutions. Although antibiotic catheter lock solutions have been present for decades, their use has been mostly limited to the treatment of CRBSIs due to concern for promoting microbial resistance. Recently, however, with the advent of non-antibiotic antimicrobial catheter lock solutions, this approach is gaining popularity as a promising method to decrease rates of CRBSI in HPN patients.
Quality of Life of Celiac Patients in Brazil: Questionnaire Translation, Cultural Adaptation and Validation
Authors: Pratesi Claudia , Häuser Winfred , Uenishi Rosa , Selleski Nicole , Nakano Eduardo , Gandolfi Lenora , Pratesi Riccardo , Zandonadi Renata
Published: August 2018

(1) Background: Celiac disease (CD) is a common autoimmune disorder. The manifestations of the disease and the obligatory life-long gluten-free diet (GFD) are associated with the impairment of patients’ quality of life. Therefore, the present study aimed to translate, culturally adapt and validate a celiac disease quality of life (CD-QoL) questionnaire and apply it to a representative number of Brazilian CD patients. (2) Methods: A cross-cultural Brazilian-Portuguese version of the CD-QoL was developed according to revised international guidelines. The questionnaire was administered to 450 celiac patients. The reliability, reproducibility and validity were studied. (3) Results: The Brazilian CD-QoL questionnaire presents valid measures of reproducibility and internal consistency. Early diagnosis is related to higher scores of Brazilian CD-QoL social, sub- and total scale. There was a positive correlation between higher education level and higher QoL. Individuals with partners tend to have a better emotional subscale of QoL. CD-patients who follow a strict GFD have highest QoL scale values. Men scored higher than women on the CD-QoL. All results were statistically significant except for the gastrointestinal subscale. (4) Conclusions: Brazilian CD-QoL allows comparative research between different celiac populations in the world. QoL research will help in the development of effective strategies to improve Brazilian celiac patients’ quality of life.
Impact of Inflammation on Ferritin, Hepcidin and the Management of Iron Deficiency Anemia in Chronic Kidney Disease
Authors: Ueda Norishi , Takasawa Kazuya
Published: August 2018

Iron deficiency anemia (IDA) is a major problem in chronic kidney disease (CKD), causing increased mortality. Ferritin stores iron, representing iron status. Hepcidin binds to ferroportin, thereby inhibiting iron absorption/efflux. Inflammation in CKD increases ferritin and hepcidin independent of iron status, which reduce iron availability. While intravenous iron therapy (IIT) is superior to oral iron therapy (OIT) in CKD patients with inflammation, OIT is as effective as IIT in those without. Inflammation reduces predictive values of ferritin and hepcidin for iron status and responsiveness to iron therapy. Upper limit of ferritin to predict iron overload is higher in CKD patients with inflammation than in those without. However, magnetic resonance imaging studies show lower cutoff levels of serum ferritin to predict iron overload in dialysis patients with apparent inflammation than upper limit of ferritin proposed by international guidelines. Compared to CKD patients with inflammation, optimal ferritin levels for IDA are lower in those without, requiring reduced iron dose and leading to decreased mortality. The management of IDA should differ between CKD patients with and without inflammation and include minimization of inflammation. Further studies are needed to determine the impact of inflammation on ferritin, hepcidin and therapeutic strategy for IDA in CKD.
A Novel Protocol Using Small-Scale Spray-Drying for the Efficient Screening of Solid Dispersions in Early Drug Development and Formulation, as a Straight Pathway from Screening to Manufacturing Stages
Authors: Ousset Aymeric , Chirico Rosanna , Robin Florent , Schubert Martin Alexander , Somville Pascal , Dodou Kalliopi
Published: August 2018

This work describes a novel screening strategy that implements small-scale spray-drying in early development of binary amorphous solid dispersions (ASDs). The proposed methodology consists of a three-stage decision protocol in which small batches (20–100 mg) of spray-dried solid dispersions (SDSDs) are evaluated in terms of drug–polymer miscibility, physical stability and dissolution performance in bio-predictive conditions. The objectives are to select the adequate carrier and drug-loading (DL) for the manufacturing of robust SDSD; and the appropriate stabilizer dissolved in the liquid vehicle of SDSD suspensions, which constitutes the common dosage form used during non-clinical studies. This methodology was verified with CDP146, a poorly water soluble (<2 µg/mL) API combined with four enteric polymers and four stabilizers. CDP146/HPMCAS-LF 40:60 (w/w) and 10% (w/v) PVPVA were identified as the lead SDSD and the best performing stabilizer, respectively. Lead SDSD suspensions (1–50 mg/mL) were found to preserve complete amorphous state during 8 h and maintain supersaturation in simulated rat intestinal fluids during the absorption window. Therefore, the implementation of spray-drying as a small-scale screening approach allowed maximizing screening effectiveness with respect to very limited API amounts (735 mg) and time resources (9 days), while removing transfer steps between screening and manufacturing phases.
Effect of 5-Oxo-2-Pyrrolidinecarboxylic Acid (PCA) as a New Topically Applied Agent for Dry Eye Syndrome Treatment
Authors: Tampucci Silvia , Monti Daniela , Burgalassi Susi , Terreni Eleonora , Zucchetti Erica , Baldacci Filippo , Chetoni Patrizia
Published: August 2018

The aim of the study was the evaluation of the suitability of 5-oxo-2-pyrrolidinecarboxylic acid (PCA), also in combination with hyaluronic acid (HA), as artificial tears for treatment of dry eye syndrome (DES). Different aqueous formulations containing 0.10% w/w of PCA were used to determine: (i) ex vivo permeation profile of PCA in isolated rabbit corneas; (ii) in vivo residence time of PCA in the precorneal area of rabbits; and (iii) in vivo ability of PCA to counteract the reduction of tear production in an experimental model of DES induced in rabbits. The pharmacokinetic profile of PCA in tear fluid was characterized by high concentrations immediately after application, followed by a rapid decrease, with half-life values of 17.16 and 22.27 min for solutions containing PCA alone and in combination with HA, respectively, when 100 µL of solutions were instilled. The addition of HA almost doubled the PCA bioavailability minimizing the ex vivo apparent corneal permeability of PCA. A positive Shirmer Test Score (STS) was observed for PCA compared to contralateral eyes at all days of treatment for PCA/HA formulation. PCA provides protection from desiccation probably for its osmoprotective activity and high water–binding capability, and this behaviour was enhanced by HA.
Stabilization of the CD81 Large Extracellular Loop with De Novo Disulfide Bonds Improves Its Amenability for Peptide Grafting
Authors: Vogt Stefan , Stadlmayr Gerhard , Stadlbauer Katharina , Sádio Flávio , Andorfer Peter , Grillari Johannes , Rüker Florian , Wozniak-Knopp Gordana
Published: August 2018

Tetraspan proteins are significantly enriched in the membranes of exosomal vesicles (EVs) and their extracellular domains are attractive targets for engineering towards specific antigen recognition units. To enhance the tolerance of a tetraspanin fold to modification, we achieved significant thermal stabilization of the human CD81 large extracellular loop (hCD81 LEL) via de novo disulfide bonds. The best mutants were shown to exhibit a positive shift in the melting temperature (Tm) of up to 25 °C. The combination of two most potent disulfide bonds connecting different strands of the protein resulted in a mutant with a Tm of 109 °C, 43 °C over the Tm of the wild-type hCD81 LEL. A peptide sequence binding to the human transferrin receptor (hTfr) was engrafted into the D-segment of the hCD81 LEL, resulting in a mutant that still exhibited a compact fold. Grafting of the same peptide sequence between helices A and B resulted in a molecule with an aberrant profile in size exclusion chromatography (SEC), which could be improved by a de novo cysteine bond connecting both helices. Both peptide-grafted proteins showed an enhanced internalization into the cell line SK-BR3, which strongly overexpresses hTfr. In summary, the tetraspan LEL fold could be stabilized to enhance its amenability for engineering into a more versatile protein scaffold.
Evaluation of the Bactericidal and Fungicidal Activities of Poly([2-(methacryloyloxy)ethyl]trimethyl Ammonium Chloride)(Poly (METAC))-Based Materials
Authors: Shiga Toshiki , Mori Hiromitsu , Uemura Keiichi , Moriuchi Ryota , Dohra Hideo , Yamawaki-Ogata Aika , Narita Yuji , Saito Akihiro , Kotsuchibashi Yohei
Published: August 2018

Poly([2-(methacryloyloxy)ethyl]trimethyl ammonium chloride) (METAC) and the gels were prepared and evaluated for their bactericidal and fungicidal activities. The antimicrobial properties of poly(METAC) were tested against Escherichia coli (E. coli), Bacillus subtilis (B. subtilis), Saccharomyces cerevisiae (Sa. cerevisiae), methicillin-susceptible Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), and Candida albicans (C. albicans). Moreover, the structural forms of the linear and cross-linked poly(METAC) were investigated for their influences on bacterial aggregation, precipitation, and cell-death. To our knowledge, this is the first report on the comparison of the antimicrobial properties of poly(METAC) and poly(METAC)-gels. The bactericidal and fungicidal activities were evaluated by determining minimum inhibitory concentrations (MICs), UV–Vis spectroscopy, and fluorescence and confocal microscopies. The MICs were found to be 123 (MSSA), 123 (MRSA), 123 (P. aeruginosa), 370 (E. coli), 123 (B. subtilis), 370 (C. albicans), and 370 μg/mL (Sa. cerevisiae), as determined by broth dilution, and 370 (MSSA), 370 (MRSA), 370 (P. aeruginosa), 3300 (E. coli), 370 (B. subtilis), 1100 (C. albicans), and >10,000 μg/mL (Sa. cerevisiae), as determined by paper disc diffusion (on solid medium). The poly(METAC)-gels achieved rapid adsorption/precipitation of bacteria via the cationic surface charge. Thus, these poly(METAC)-based polymers can potentially be used as antibacterial materials.
Transformation from Real-Centredness to Other-Centredness: A Levinasian Re-Appraisal of John Hick’s Religious Pluralism
Authors: Mao Xin
Published: August 2018

John Hick’s theory of religious pluralism has from its birth faced critiques regarding both its conceptual framework and its religious outlook; yet even so, his philosophy continues to challenge us to strive for a greater sense of openness and equality as regards other faiths that conflict with our own. The viability of Hick’s teaching today depends on a re-appraisal that enables it to surmount its theoretical difficulties. In this paper, we re-evaluate Hick’s philosophy of religion, focusing on the underlying ethical importance of his claim regarding soteriological transformation. Despite the problematic notion of the noumenal Real and its role in religious pluralism, the soteriological transformation claimed by Hick, which goes from self-centredness toward Real-centredness, reveals a commitment to self-opening and compassion towards the others. Yet we will argue that Hick only gives this ethical importance a secondary status in his philosophy of religion, which leaves open the question of the nature of the causality between the ultimate Reality and this ethical commitment. We thereby engage with the philosophy of Emmanuel Levinas, in search of an ethical dimension with a characteristic of infinity, which can offer religious pluralism a transcendent foundation without disregarding ethical primacy. Following Levinas, we will argue for a further transformation from reality-centredness towards other-centredness, by which messianic peace would take the place of ultimate Reality as the teleological value underpinning religious pluralism.
An Analysis of Factors Influencing the Relationship between Satellite-Derived AOD and Ground-Level PM10
Authors: Stirnberg Roland , Cermak Jan , Andersen Hendrik
Published: August 2018

Air pollution can endanger human health, especially in urban areas. Assessment of air quality primarily relies on ground-based measurements, but these provide only limited information on the spatial distribution of pollutants. In recent years, satellite derived Aerosol Optical Depth (AOD) has been used to approximate particulate matter (PM) with varying success. In this study, the relationship between hourly mean concentrations of particulate matter with a diameter of 10 micrometers or less (PM10) and instantaneous AOD measurements is investigated for Berlin, Germany, for 2001–2015. It is found that the relationship between AOD and PM10 is rarely linear and strongly influenced by ambient relative humidity (RH), boundary layer height (BLH), wind direction and wind speed. Generally, when a moderately dry atmosphere (30% < RH ≤ 50%) coincides with a medium BLH (600–1200 m), AOD and PM10 are in the same range on a semi-quantitative scale. AOD increases with ambient RH, leading to an overestimation of the dry particle concentration near ground. However, this effect can be compensated if a low boundary layer (<600 m) is present, which in turn significantly increases PM10, eventually leading to satellite AOD and PM10 measurements of similar magnitude. Insights of this study potentially influence future efforts to estimate near-ground PM concentrations based on satellite AOD.
Airborne and Terrestrial Laser Scanning Data for the Assessment of Standing and Lying Deadwood: Current Situation and New Perspectives
Authors: Marchi Niccolò , Pirotti Francesco , Lingua Emanuele
Published: August 2018

LiDAR technology is finding uses in the forest sector, not only for surveys in producing forests but also as a tool to gain a deeper understanding of the importance of the three-dimensional component of forest environments. Developments of platforms and sensors in the last decades have highlighted the capacity of this technology to catch relevant details, even at finer scales. This drives its usage towards more ecological topics and applications for forest management. In recent years, nature protection policies have been focusing on deadwood as a key element for the health of forest ecosystems and wide-scale assessments are necessary for the planning process on a landscape scale. Initial studies showed promising results in the identification of bigger deadwood components (e.g., snags, logs, stumps), employing data not specifically collected for the purpose. Nevertheless, many efforts should still be made to transfer the available methodologies to an operational level. Newly available platforms (e.g., Mobile Laser Scanner) and sensors (e.g., Multispectral Laser Scanner) might provide new opportunities for this field of study in the near future.
Development of Shoreline Extraction Method Based on Spatial Pattern Analysis of Satellite SAR Images
Authors: Fuse Takashi , Ohkura Takashi
Published: August 2018

The extensive monitoring of shorelines is becoming important for investigating the impact of coastal erosion. Satellite synthetic aperture radar (SAR) images can cover wide areas independently of weather or time. The recent development of high-resolution satellite SAR images has made observations more detailed. Shoreline extraction using high-resolution images, however, is challenging because of the influence of speckle, crest lines, patterns in sandy beaches, etc. We develop a shoreline extraction method based on the spatial pattern analysis of satellite SAR images. The proposed method consists of image decomposition, smoothing, sea and land area segmentation, and shoreline refinement. The image decomposition step, in which the image is decomposed into its texture and outline components, is based on morphological component analysis. In the image decomposition step, a learning process involving spatial patterns is introduced. The outline images are smoothed using a non-local means filter, and then the images are segmented into sea and land areas using the graph cuts’ technique. The boundary between these two areas can be regarded as the shoreline. Finally, the snakes algorithm is applied to refine the position accuracy. The proposed method is applied to the satellite SAR images of coasts in Japan. The method can successfully extract the shorelines. Through experiments, the performance of the proposed method is confirmed.