bluuebunny/crossref_metadata_2025 huggingface dataset:
Archeoseismic Study of Damage in Medieval Monuments around New Delhi, India: An Approach to Understanding Paleoseismicity in an Intraplate Region
Authors: Naik Sambit Prasanajit , Reicherter Klaus , Kázmér Miklos , Skapski Jens , Mohanty Asmita , Kim Young-Seog
Published: February 2024

The seismic shaking observed around Delhi and the surrounding region due to near-field and far-field earthquakes is a matter of concern for the seismic safety of the national capital of India, as well as the historical monuments of the region. Historical seismicity indicates that the Delhi region has been affected by several damaging earthquakes originating from the Himalayan region as far-field events, as well as due to near-field earthquakes with epicenters close to Delhi. The historical records, along with recent archeoseismological studies, suggest that Qutab Minar, a UNESCO World Heritage Site, was damaged by the earthquake of 1803 CE. This event represents the only evidence of seismic damage from the region, as there has been no detailed study of other historical monuments in the area or earthquakes that have caused damage. In this context, the earthquake damage to other monuments might have been overlooked to some extent around the Qutab Minar due to the lack of proper earthquake damage surveys and documentation in historical times. The main goal of this study is to identify evidence of earthquake archeological effects around the Qutab Minar and to shed new light on the occurrence and characteristics of ancient earthquakes while providing data to inform seismic risk assessment programs. With this aim, we describe different earthquake-related damage (EAE, earthquake archeological effects) at the Isa Khan Tomb and Humayun’s Tomb, built between 1548 CE and 1570 CE, respectively, as well as the older Tomb of Iltutmish (built in 1235 CE) along with the Qutab Minar, which was built between 1199 CE and 1220 CE. The damage was probably caused by seismic events with intensities between VIII and IX on the European Macroseismic Scale (EMS). Based on the methodology of paleo ShakeMaps, it is most likely that the 1803 CE earthquake was the causative earthquake for the observed deformation in the Isa Khan Tomb, Tomb of Iltutmish, and Humayun’s Tomb. More detailed regional paleoseismological studies are required to identify the responsible fault. In conclusion, the impressive cultural heritage of Delhi city and the intraplate region is constantly under seismic threats from near-field earthquakes and far-field Himalayan earthquakes.
Transcriptional Insights into Lily Stem Bulblet Formation: Hormonal Regulation, Sugar Metabolism, and Transcriptional Networks in LA Lily ‘Aladdin’
Authors: Zhang Kewen , Lyu Tong , Lyu Yingmin
Published: February 2024

Bulblets, as the main reproductive organ of lilies, have a tremendous impact on the reproductive efficiency of lilies. Cytokinin is known to promote the formation of lily bulblets, but little is known about the mechanisms involved. In this study, a combination of full-length transcriptome and high-throughput RNA sequencing (RNA-Seq) was performed at the leaf axils of LA lily ‘Aladdin’ to characterize the transcriptional response to 6-BA treatment during the critical period of stem-to-bulblet transition. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that 6-BA treatment caused significant changes in starch and sucrose metabolism and plant hormone balance. In particular, the high expression of SUS1 and TPS6 in the 6-BA-treated group suggests that sucrose may act as a key signal to promote bulblet initiation. Furthermore, the induction of elevated expression of genes associated with cytokinin and auxin transport and signaling is crucial for initiating bulblet emergence and stimulating growth. WGCNA analysis revealed that hub TFs such as BLHs, ARFs, HD-ZIPs, AP2/ERFs, and SBPs were significantly overexpressed with genes involved in carbohydrate metabolism and phytohormone signaling, which warranted more in-depth functional studies. This study enriches the understanding of plant hormone-related genes, sugar metabolism-related genes and various transcription factors in the regulation of plant organ development, and lays the foundation for further studies on the molecular mechanisms of lily stem bulblet formation.
Improving the Nutrient Management of an Apple Orchard by Using Organic-Based Composites Derived from Agricultural Waste
Authors: Tóth Florence Alexandra , Magyar Tamás , Tamás János , Nagy Péter Tamás
Published: February 2024

Extreme weather and the declining organic matter content of soils cause serious sustainability problems in agriculture. Therefore, soil conditioner composites (chicken manure, bentonite and super absorbent polymer) were developed and tested in an integrated apple orchard characterized by poor nutrient and water management to study their effects on soil, leaf and fruit attributes. Composites with higher doses of additives increased soil organic carbon by 4–9 g/kg, and organic nitrogen by 1.8–2.8 g/kg compared to the control (p < 0.05). Similarly, soil nitrate content steadily increased from 8–10 mg/kg to 30–38 mg/kg by composites. Composites effectively elevated leaf N, K, Ca, and Mg while not affecting the leaf P (p < 0.05). Treatments significantly enhanced the yields by 14–63% on average compared to the control. Treatments with bentonite improved the fruit weight by 2% and 24% compared to the chicken manure. On average, composite treatments increased the titratable acidity of fruits by 26–43% compared to the control and 0.5–10% compared to the treatment containing solely chicken manure. Overall, the developed organic-based composites are able to cope with changing circumstances that could help mitigate the negative effects of climate change, especially in arid areas, thus contributing to sustainable nutrient management.
Speaking Truth to Power: Toward a Forensic Anthropology of Advocacy and Activism
Authors: Adams Donovan M. , Bedard Juliette R. , Blatt Samantha H. , Faisal Eman , Goliath Jesse R. , Gregory-Alcock Grace , Gruenthal-Rankin Ariel , Morales Lorenzo Patricia N. , Smith Ashley C. , Tallman Sean D. , Tegtmeyer Hawke Rylan , Whitelaw Hannah
Published: February 2024

Over the years, the field of forensic anthropology has become more diverse, bringing unique perspectives to a previously homogeneous field. This diversification has been accompanied by recognizing the need for advocacy and activism in an effort to support the communities we serve: marginalized communities that are often overrepresented in the forensic population. As such, forensic anthropologists see the downstream effects of colonialism, white supremacy, inequitable policies, racism, poverty, homophobia, transphobia, gun violence, and misogyny. Some argue that advocacy and activism have no place in forensic anthropological praxis. The counterarguments for engaging in advocacy and activism uphold white, heterosexual, cisgender, and ableist privilege by arguing that perceived objectivity and unbiased perspectives are more important than personally biasing experiences and positionality that supposedly jeopardize the science and expert testimony. Advocacy and activism, however, are not new to the practice of anthropology. Whether through sociocultural anthropology, archaeology, or other areas of biological anthropology, activism and advocacy play an important role, using both the scientific method and community engagement. Using a North American approach, we detail the scope of the issues, address how advocacy and activism are perceived in the wider discipline of anthropology, and define ways in which advocacy and activism can be utilized more broadly in the areas of casework, research, and education.
Long-Term Spatiotemporal Investigation of Various Rainfall Intensities over Central India Using EO Datasets
Authors: Awasthi Nitesh , Tripathi Jayant Nath , Petropoulos George P. , Kumar Pradeep , Singh Abhay Kumar , Dakhore Kailas Kamaji , Ghosh Kripan , Gupta Dileep Kumar , Srivastava Prashant K. , Kalogeropoulos Kleomenis , Singh Sartajvir , Singh Dhiraj Kumar
Published: February 2024

This study involved an investigation of the long-term seasonal rainfall patterns in central India at the district level during the period from 1991 to 2020, including various aspects such as the spatiotemporal seasonal trend of rainfall patterns, rainfall variability, trends of rainy days with different intensities, decadal percentage deviation in long-term rainfall patterns, and decadal percentage deviation in rainfall events along with their respective intensities. The central region of India was meticulously divided into distinct subparts, namely, Gujarat, Daman and Diu, Maharashtra, Goa, Dadra and Nagar Haveli, Madhya Pradesh, Chhattisgarh, and Odisha. The experimental outcomes represented the disparities in rainfall distribution across different districts of central India with the spatial distribution of mean rainfall ranges during winter (2.08 mm over Dadra and Nagar Haveli with an average of 24.19 mm over Odisha), premonsoon (6.65 mm over Gujarat to 132.89 mm over Odisha), monsoon (845.46 mm over Gujarat to 3188.21 mm over Goa), and post-monsoon (30.35 mm over Gujarat to 213.87 mm over Goa), respectively. Almost all the districts of central India displayed an uneven pattern in the percentage deviation of seasonal rainfall in all three decades for all seasons, which indicates the seasonal rainfall variability over the last 30 years. A noticeable variation in the percentage deviation of seasonal rainfall patterns has been observed in the following districts: Rewa, Puri, Anuppur, Ahmadabad, Navsari, Chhindwara, Devbhumi Dwarka, Amreli, Panch Mahals, Kolhapur, Kandhamal, Ratnagiri, Porbandar, Bametara, and Sabar Kantha. In addition, a larger number of rainy days of various categories occurred in the monsoon season in comparison to other seasons. A higher contribution of trace rainfall events was found in the winter season. The highest contributions of very light, light rainfall, moderate, rather high, and high events were found in the monsoon season in central India. The percentage of various categories of rainfall events has decreased over the last two decades (2001–2020) in comparison to the third decade (1991–2000), according to the mean number of rainfall events in the last 30 years. This spatiotemporal analysis provides valuable insights into the rainfall trends in central India, which represent regional disparities and the potential challenges impacted by climate patterns. This study contributes to our understanding of the changing rainfall dynamics and offers crucial information for effective water resource management in the region.
Sensitivity of Groundwater Recharge Assessment to Input Data in Arid Areas
Authors: Ajjur Salah Basem , Di Lorenzo Emanuele
Published: February 2024

Natural groundwater recharge (GR) assessment depends on several hydrogeological and climatic inputs, where uncertainty is inevitable. Assessing how inputs’ uncertainty affects GR estimation is important; however, it remains unclear in arid areas. This study assesses inputs’ uncertainty by examining the changes in GR simulations resulting from modifications in climatic, land use, and soil inputs. A physical-based hydrological model was built to estimate GR from 18 different GR scenarios across Qatar. Scenarios S1–S7 were created from different climatic inputs but identical land use and soil maps. Scenarios S8–S14 were created from different land use maps (analyzed from historical Landsat satellite images) but similar climatic and soil inputs. In S15–S18, the soil parameters were changed while the climatic and land use maps were kept the same. The results show that climatic inputs are key factors controlling the GR in arid areas, followed by land use inputs and soil classification. A strong correlation was observed between the GR values and precipitation, while moderate (non-significant) correlations were observed between the GR values and potential evapotranspiration and wind speed. Soil changes affected the GR simulations but inconsiderably compared with climatic and land use inputs. Since GR estimation is fundamental but uncertain in arid areas, the study findings contribute to narrowing the uncertainty in GR estimation.
Keeping Sane in a Changing Climate: Assessing Psychologists’ Preparedness, Exposure to Climate-Health Impacts, Willingness to Act on Climate Change, and Barriers to Effective Action
Authors: Stilita Gabriela , Charlson Fiona
Published: February 2024

Evidence of the impact of climate change on mental health is growing rapidly, and healthcare professionals are being called to be active participants in protecting the population’s health. Yet, little is known about psychologists’ understanding of climate-health impacts and their role in mitigation actions. We surveyed Australian psychologists (N = 59) to examine preparedness in identifying and managing the impact of climate change on mental health, exposure to climate-health impacts, willingness to act, and barriers to acting on climate change. Data was analysed through descriptive and associative methods. We found that participants are not prepared to identify and manage mental health presentations related to climate change, and they are not engaged in climate change mitigation. We identified that a lack of knowledge of climate-health impacts and tackling and mitigation strategies, in addition to ethical concerns, were the main barriers to engagement with communication and advocacy. With the impacts of climate change on mental health expected to soar, there is a clear and urgent need to prepare the psychological workforce to address this public health issue by establishing professional education programs and reframing climate change as a health crisis.
Integrating Mental Health Management into Empowerment Group Sessions for Out-of-School Adolescents in Kenyan Informal Settlements: A Process Paper
Authors: Mutahi Joan , Kangwana Beth , Khasowa Dorcas , Muthoni Irene , Charo Oliver , Muli Alfred , Kumar Manasi
Published: February 2024

This article presents processes for developing contextualized training procedures to better appreciate partnership, capacity-building experiences, and specific implementation challenges and opportunities for mental and public health teams. The program enrolled 469 out-of-school adolescents to participate in the integration of youth mental health into health and life-skill safe spaces. The teams utilized various methods to achieve process outcomes of restructuring and adapting curricula, training youth mentors, and assessing their self-efficacy before integrating the intervention for 18 months. The Coronavirus (COVID-19) pandemic became an additional unique concern in the preliminary and the 18-month implementation period of the program. This necessitated innovation around hybrid training and asynchronous modalities as program teams navigated the two study locations for prompt training, supervision, evaluation, and feedback. In conclusion, out-of-school adolescents face a myriad of challenges, and a safe space program led by youth mentors can help promote mental health. Our study demonstrated how best this can be achieved. We point to lessons such as the importance of adapting the intervention and working cohesively in teams, building strong and trusting partnerships, learning how to carry out multidisciplinary dialogues, and continuous supervision and capacity building. This article aimed to document the processes around the design and implementation of this innovative intervention and present a summary of lessons learned.
Fall Risk Prediction for Community-Dwelling Older Adults: Analysis of Assessment Scale and Evaluation Items without Actual Measurement
Authors: Murayama Akihiko , Higuchi Daisuke , Saida Kosuke , Tanaka Shigeya , Shinohara Tomoyuki
Published: February 2024

The frequency of falls increases with age. In Japan, the population is aging rapidly, and fall prevention measures are an urgent issue. However, assessing fall risk during the coronavirus disease pandemic was complicated by the social distancing measures implemented to prevent the disease, while traditional assessments that involve actual measurements are complicated. This prospective cohort study predicted the risk of falls in community-dwelling older adults using an assessment method that does not require actual measurements. A survey was conducted among 434 community-dwelling older adults to obtain data regarding baseline attributes (age, sex, living with family, use of long-term care insurance, and multimorbidity), Frailty Screening Index (FSI) score, and Questionnaire for Medical Checkup of Old-Old (QMCOO) score. The participants were categorized into fall (n = 78) and non-fall (n = 356) groups. The binomial logistic regression analysis showed that it is better to focus on the QMCOO sub-item score, which focuses on multiple factors. The items significantly associated with falls were Q5 (odds ratio [OR] 1.95), Q8 (OR 2.33), and Q10 (OR 3.68). Our results were similar to common risk factors for falls in normal times. During the pandemic, being able to gauge the risk factors for falls without actually measuring them was important.
Physical Activity, Sedentary Time, and Psychosocial Functioning among Adults with Cancer: A Scoping Review
Authors: Côté Arianne , Miquelon Paule , Trudel-Fitzgerald Claudia
Published: February 2024

The post-treatment period (after the completion of primary cancer treatment) is a phase during which adults with cancer are particularly vulnerable to the physical and psychological side effects of treatment. Adopting healthy lifestyle habits during this time is essential to mitigate these effects. This scoping review investigated the associations of physical activity (PA) and sedentary time (ST) with two post-treatment psychosocial indicators among adults with cancer: psychological functioning and quality of life (QoL). An exhaustive search was performed in January 2023 across five databases, namely APA PsycInfo, MedLine, SPORTDiscuss, SCOPUS, and CINAHL, adhering to PRISMA guidelines for scoping reviews. Twenty articles met the inclusion criteria; 16 used a cross-sectional design, while 4 used a longitudinal one. PA and ST were assessed mainly with accelerometers (n = 17), and psychosocial indicators with self-reported questionnaires (n = 20). Most studies linked higher PA levels to reduced anxiety (n = 3) and depression (n = 4) symptoms, and elevated ST to higher psychological symptoms (n = 3). Opposite associations were observed for QoL (n = 5). Altogether, PA appeared to be more strongly related to psychological functioning and QoL than ST. This scoping review highlights associations of PA and ST with psychological functioning and QoL among adults with cancer in the post-treatment period. However, future studies must prioritize longitudinal designs to establish directionality.
Methodological Innovations for Establishing Cemetery Spatial Databases—A UAV-Based Workflow Helping Small Communities
Authors: Pál Márton , Hajdú Edina
Published: February 2024

Various modern large-scale mapping techniques have already been introduced in earth sciences, cadastral mapping, and the agricultural sector. These methodologies often use remotely sensed data to compile various analogue or digital cartographic products as well as spatial databases. However, the mapping of cemeteries and standards for establishing a spatial database for them have rarely been published, and there is no definite method for this purpose in Hungary yet. We have compiled a methodology based on mapping experiences in three sample areas in the Roman Catholic Archdiocese of Eger in Hungary that are church properties. The initial UAV-based fieldwork orthomosaics were processed with a CV (computer vision)-based script that vectorised grave contours. After fieldwork, which included the recording of the deceased people’s names and their dates of birth and death in the case of all graves, a spatial database was created pairing each polygon with the corresponding personal data. A map was also generated from the results of the survey. The cartographic product and the database fulfil legal requirements and give hints for cemeteries regarding further planning. The developed method is capable of making mapping and database building easier—not just in the case of graves, but with other rectangular objects, too.
Study on LOS to Vertical Deformation Conversion Model on Embankment Slopes Using Multi-Satellite SAR Interferometry
Authors: Liu Jie , Li Tao , Ma Sijie , Shan Qiang , Jiang Weiping
Published: February 2024

Slant range geometry plays a crucial role in interpreting synthetic aperture radar (SAR) observations, especially in converting line-of-sight (LOS) surface deformations to actual vertical subsidence. This paper proposes a new conversion model to retrieve vertical settlements of the embankment slopes using the geometrical parameters of the dam and the SAR sensor. The simulation results highlight the impact of slope foreshortening and heading direction of the satellite on deformation retrieval. Various SAR data with different resolutions and bands are used to analyze the model’s performance, revealing a high conformity of the model with practical conversion parameters exceeding 80% for TerraSAR-X and Cosmo-SkyMed data.
Involvement of Mitochondria in the Selective Response to Microsecond Pulsed Electric Fields on Healthy and Cancer Stem Cells in the Brain
Authors: Casciati Arianna , Taddei Anna Rita , Rampazzo Elena , Persano Luca , Viola Giampietro , Cani Alice , Bresolin Silvia , Cesi Vincenzo , Antonelli Francesca , Mancuso Mariateresa , Merla Caterina , Tanori Mirella
Published: February 2024

In the last few years, pulsed electric fields have emerged as promising clinical tools for tumor treatments. This study highlights the distinct impact of a specific pulsed electric field protocol, PEF-5 (0.3 MV/m, 40 μs, 5 pulses), on astrocytes (NHA) and medulloblastoma (D283) and glioblastoma (U87 NS) cancer stem-like cells (CSCs). We pursued this goal by performing ultrastructural analyses corroborated by molecular/omics approaches to understand the vulnerability or resistance mechanisms triggered by PEF-5 exposure in the different cell types. Electron microscopic analyses showed that, independently of exposed cells, the main targets of PEF-5 were the cell membrane and the cytoskeleton, causing membrane filopodium-like protrusion disappearance on the cell surface, here observed for the first time, accompanied by rapid cell swelling. PEF-5 induced different modifications in cell mitochondria. A complete mitochondrial dysfunction was demonstrated in D283, while a mild or negligible perturbation was observed in mitochondria of U87 NS cells and NHAs, respectively, not sufficient to impair their cell functions. Altogether, these results suggest the possibility of using PEF-based technology as a novel strategy to target selectively mitochondria of brain CSCs, preserving healthy cells.
scMGCN: A Multi-View Graph Convolutional Network for Cell Type Identification in scRNA-seq Data
Authors: Sun Hongmin , Qu Haowen , Duan Kaifu , Du Wei
Published: February 2024

Single-cell RNA sequencing (scRNA-seq) data reveal the complexity and diversity of cellular ecosystems and molecular interactions in various biomedical research. Hence, identifying cell types from large-scale scRNA-seq data using existing annotations is challenging and requires stable and interpretable methods. However, the current cell type identification methods have limited performance, mainly due to the intrinsic heterogeneity among cell populations and extrinsic differences between datasets. Here, we present a robust graph artificial intelligence model, a multi-view graph convolutional network model (scMGCN) that integrates multiple graph structures from raw scRNA-seq data and applies graph convolutional networks with attention mechanisms to learn cell embeddings and predict cell labels. We evaluate our model on single-dataset, cross-species, and cross-platform experiments and compare it with other state-of-the-art methods. Our results show that scMGCN outperforms the other methods regarding stability, accuracy, and robustness to batch effects. Our main contributions are as follows: Firstly, we introduce multi-view learning and multiple graph construction methods to capture comprehensive cellular information from scRNA-seq data. Secondly, we construct a scMGCN that combines graph convolutional networks with attention mechanisms to extract shared, high-order information from cells. Finally, we demonstrate the effectiveness and superiority of the scMGCN on various datasets.
Conformational Dynamics of Lipoxygenases and Their Interaction with Biological Membranes
Authors: Erba Fulvio , Mei Giampiero , Minicozzi Velia , Sabatucci Annalaura , Di Venere Almerinda , Maccarrone Mauro
Published: February 2024

Lipoxygenases (LOXs) are a family of enzymes that includes different fatty acid oxygenases with a common tridimensional structure. The main functions of LOXs are the production of signaling compounds and the structural modifications of biological membranes. These features of LOXs, their widespread presence in all living organisms, and their involvement in human diseases have attracted the attention of the scientific community over the last decades, leading to several studies mainly focused on understanding their catalytic mechanism and designing effective inhibitors. The aim of this review is to discuss the state-of-the-art of a different, much less explored aspect of LOXs, that is, their interaction with lipid bilayers. To this end, the general architecture of six relevant LOXs (namely human 5-, 12-, and 15-LOX, rabbit 12/15-LOX, coral 8-LOX, and soybean 15-LOX), with different specificity towards the fatty acid substrates, is analyzed through the available crystallographic models. Then, their putative interface with a model membrane is examined in the frame of the conformational flexibility of LOXs, that is due to their peculiar tertiary structure. Finally, the possible future developments that emerge from the available data are discussed.
Transcriptome Analysis Reveals the Molecular Mechanisms of BR Negative Regulatory Factor StBIN2 Maintaining Tuber Dormancy
Authors: Liu Shifeng , Cai Chengcheng , Li Liqin , Yu Liping , Wang Qiang , Wang Xiyao
Published: February 2024

Potato is an important food crop. After harvest, these tubers will undergo a period of dormancy. Brassinosteroids (BRs) are a new class of plant hormones that regulate plant growth and seed germination. In this study, 500 nM of BR was able to break the dormancy of tubers. Additionally, exogenous BR also upregulated BR signal transduction genes, except for StBIN2. StBIN2 is a negative regulator of BR, but its specific role in tuber dormancy remains unclear. Transgenic methods were used to regulate the expression level of StBIN2 in tubers. It was demonstrated that the overexpression of StBIN2 significantly prolonged tuber dormancy while silencing StBIN2 led to premature sprouting. To further investigate the effect of StBIN2 on tuber dormancy, RNA-Seq was used to analyze the differentially expressed genes in OE-StBIN2, RNAi-StBIN2, and WT tubers. The results showed that StBIN2 upregulated the expression of ABA signal transduction genes but inhibited the expression of lignin synthesis key genes. Meanwhile, it was also found that StBIN2 physically interacted with StSnRK2.2 and StCCJ9. These results indicate that StBIN2 maintains tuber dormancy by mediating ABA signal transduction and lignin synthesis. The findings of this study will help us better understand the molecular mechanisms underlying potato tuber dormancy and provide theoretical support for the development of new varieties using related genes.
Dihydromyrcenol Modulates Involucrin Expression through the Akt Signaling Pathway
Authors: Yang Suhjin , Kang Wesuk , Choi Dabin , Roh Jiyun , Park Taesun
Published: February 2024

The epidermis serves as a protective barrier against external threats and is primarily composed of keratinocytes, which ultimately form corneocytes. Involucrin, a protein integral to the cornified envelope, plays a pivotal role in preserving the functional integrity of the skin barrier. Previous studies have shown that Akt plays an important role in keratinocyte differentiation and skin barrier development. This study investigated whether dihydromyrcenol (DHM), a plant-derived terpene, could increase involucrin production in keratinocytes and sought to elucidate the possible underlying mechanisms. To accomplish this objective, we assessed the alterations in involucrin by DHM through quantitative PCR and Western blot on the HaCaT cell line. The changes in the promoter levels were investigated using luciferase assays. Furthermore, upstream mechanisms were explored through the use of siRNA and inhibitors. To strengthen our findings, the results were subsequently validated in primary cells and 3D skin equivalents. DHM significantly increased involucrin mRNA and protein levels in a concentration-dependent manner. In addition, the Fyn-Akt signaling pathway was found to be required for DHM-induced involucrin expression, as inhibition of Fyn or Akt blocked the increase in involucrin mRNA induced by DHM. The transcription factor Sp1, which is recognized as one of the transcription factors for involucrin, was observed to be activated in response to DHM treatment. Moreover, DHM increased epidermal thickness in a 3D human skin model. These findings suggest that the modulation of involucrin expression with DHM could improve skin barrier function and highlight the importance of manipulating the Akt pathway to achieve this improvement.
Recent Analytical Methodologies in Lipid Analysis
Authors: Gerhardtova Ivana , Jankech Timotej , Majerova Petra , Piestansky Juraj , Olesova Dominika , Kovac Andrej , Jampilek Josef
Published: February 2024

Lipids represent a large group of biomolecules that are responsible for various functions in organisms. Diseases such as diabetes, chronic inflammation, neurological disorders, or neurodegenerative and cardiovascular diseases can be caused by lipid imbalance. Due to the different stereochemical properties and composition of fatty acyl groups of molecules in most lipid classes, quantification of lipids and development of lipidomic analytical techniques are problematic. Identification of different lipid species from complex matrices is difficult, and therefore individual analytical steps, which include extraction, separation, and detection of lipids, must be chosen properly. This review critically documents recent strategies for lipid analysis from sample pretreatment to instrumental analysis and data interpretation published in the last five years (2019 to 2023). The advantages and disadvantages of various extraction methods are covered. The instrumental analysis step comprises methods for lipid identification and quantification. Mass spectrometry (MS) is the most used technique in lipid analysis, which can be performed by direct infusion MS approach or in combination with suitable separation techniques such as liquid chromatography or gas chromatography. Special attention is also given to the correct evaluation and interpretation of the data obtained from the lipid analyses. Only accurate, precise, robust and reliable analytical strategies are able to bring complex and useful lipidomic information, which may contribute to clarification of some diseases at the molecular level, and may be used as putative biomarkers and/or therapeutic targets.
Transcriptome Analysis Reveals the Mechanism by Which Exogenous Melatonin Treatment Delays Leaf Senescence of Postharvest Chinese Kale (Brassica oleracea var. alboglabra)
Authors: Di Hongmei , Zhang Chenlu , Zhou Aolian , Huang Huanhuan , Tang Yi , Li Huanxiu , Huang Zhi , Zhang Fen , Sun Bo
Published: February 2024

Melatonin, a pleiotropic small molecule, is employed in horticultural crops to delay senescence and preserve postharvest quality. In this study, 100 µM melatonin treatment delayed a decline in the color difference index h* and a*, maintaining the content of chlorophyll and carotenoids, thereby delaying the yellowing and senescence of Chinese kale. Transcriptome analysis unequivocally validates melatonin’s efficacy in delaying leaf senescence in postharvest Chinese kale stored at 20 °C. Following a three-day storage period, the melatonin treatment group exhibited 1637 differentially expressed genes (DEGs) compared to the control group. DEG analysis elucidated that melatonin-induced antisenescence primarily governs phenylpropanoid biosynthesis, lipid metabolism, plant signal transduction, and calcium signal transduction. Melatonin treatment up-regulated core enzyme genes associated with general phenylpropanoid biosynthesis, flavonoid biosynthesis, and the α-linolenic acid biosynthesis pathway. It influenced the redirection of lignin metabolic flux, suppressed jasmonic acid and abscisic acid signal transduction, and concurrently stimulated auxin signal transduction. Additionally, melatonin treatment down-regulated RBOH expression and up-regulated genes encoding CaM, thereby influencing calcium signal transduction. This study underscores melatonin as a promising approach for delaying leaf senescence and provides insights into the mechanism of melatonin-mediated antisenescence in postharvest Chinese kale.
Involvement of M1-Activated Macrophages and Perforin/Granulysin Expressing Lymphocytes in IgA Vasculitis Nephritis
Authors: Laskarin Gordana , Babarovic Emina , Kifer Nastasia , Bulimbasic Stela , Sestan Mario , Held Martina , Frkovic Marijan , Gagro Alenka , Coric Marijana , Jelusic Marija
Published: February 2024

We investigated the polarisation of CD68+ macrophages and perforin and granulysin distributions in kidney lymphocyte subsets of children with IgA vasculitis nephritis (IgAVN). Pro-inflammatory macrophage (M)1 (CD68/iNOS) or regulatory M2 (CD68/arginase-1) polarisation; spatial arrangement of macrophages and lymphocytes; and perforin and granulysin distribution in CD3+ and CD56+ cells were visulaised using double-labelled immunofluorescence. In contrast to the tubules, iNOS+ cells were more abundant than the arginase-1+ cells in the glomeruli. CD68+ macrophage numbers fluctuated in the glomeruli and were mostly labelled with iNOS. CD68+/arginase-1+ cells are abundant in the tubules. CD56+ cells, enclosed by CD68+ cells, were more abundant in the glomeruli than in the tubuli, and co-expressed NKp44. The glomerular and interstitial/intratubular CD56+ cells express perforin and granulysin, respectively. The CD3+ cells did not express perforin, while a minority expressed granulysin. Innate immunity, represented by M1 macrophages and CD56+ cells rich in perforin and granulysin, plays a pivotal role in the acute phase of IgAVN.
Design and Synthesis of Pleuromutilin Derivatives as Antibacterial Agents Using Quantitative Structure–Activity Relationship Model
Authors: Zhang Jiaming , Liu Qinqin , Zhao Haoxia , Li Guiyu , Yi Yunpeng , Shang Ruofeng
Published: February 2024

The quantitative structure–activity relationship (QSAR) is one of the most popular methods for the virtual screening of new drug leads and optimization. Herein, we collected a dataset of 955 MIC values of pleuromutilin derivatives to construct a 2D-QSAR model with an accuracy of 80% and a 3D-QSAR model with a non-cross-validated correlation coefficient (r2) of 0.9836 and a cross-validated correlation coefficient (q2) of 0.7986. Based on the obtained QSAR models, we designed and synthesized pleuromutilin compounds 1 and 2 with thiol-functionalized side chains. Compound 1 displayed the highest antimicrobial activity against both Staphylococcus aureus ATCC 29213 (S. aureus) and Methicillin-resistant Staphylococcus aureus (MRSA), with minimum inhibitory concentrations (MICs) < 0.0625 μg/mL. These experimental results confirmed that the 2D and 3D-QSAR models displayed a high accuracy of the prediction function for the discovery of lead compounds from pleuromutilin derivatives.
Two-Photon-Excited FLIM of NAD(P)H and FAD—Metabolic Activity of Fibroblasts for the Diagnostics of Osteoimplant Survival
Authors: Lepekhina Tatiana B. , Nikolaev Viktor V. , Darvin Maxim E. , Zuhayri Hala , Snegerev Mikhail S. , Lozhkomoev Aleksandr S. , Senkina Elena I. , Kokhanenko Andrey P. , Lozovoy Kirill A. , Kistenev Yury V.
Published: February 2024

Bioinert materials such as the zirconium dioxide and aluminum oxide are widely used in surgery and dentistry due to the absence of cytotoxicity of the materials in relation to the surrounding cells of the body. However, little attention has been paid to the study of metabolic processes occurring at the implant–cell interface. The metabolic activity of mouse 3T3 fibroblasts incubated on yttrium-stabilized zirconium ceramics cured with aluminum oxide (ATZ) and stabilized zirconium ceramics (Y-TZP) was analyzed based on the ratio of the free/bound forms of cofactors NAD(P)H and FAD obtained using two-photon microscopy. The results show that fibroblasts incubated on ceramics demonstrate a shift towards the free form of NAD(P)H, which is observed during the glycolysis process, which, according to our assumptions, is related to the porosity of the surface of ceramic structures. Consequently, despite the high viability and good proliferation of fibroblasts assessed using an MTT test and a scanning electron microscope, the cells are in a state of hypoxia during incubation on ceramic structures. The FLIM results obtained in this work can be used as additional information for scientists who are interested in manufacturing osteoimplants.
DOCK8 Mutation in Patient with Juvenile Idiopathic Arthritis and Sjögren’s Syndrome
Authors: Opoka-Winiarska Violetta , Winiarska Natalia , Lejman Monika , Gdak Małgorzata , Gosik Krzysztof , Lewandowski Filip , Niedźwiedzka-Rystwej Paulina , Grywalska Ewelina
Published: February 2024

This study investigated the association between autoimmunity and immunodeficiency in pediatric patients, focusing on the case of a 15-year-old female diagnosed with juvenile idiopathic arthritis (JIA) and secondary Sjögren’s syndrome. The patient presented with a variety of symptoms, including joint pain, bronchial asthma, leukopenia, and skin lesions. Genetic testing revealed a de novo mutation in the DOCK8 gene, associated with DOCK8 deficiency, a condition usually associated with immunodeficiencies. The clinical course, diagnostic pathway, and treatment history are detailed, highlighting the importance of molecular diagnostics in understanding the genetic basis of rheumatic diseases. This case highlights the need to consider innate immune errors in patients with multiple diseases or atypical symptoms of rheumatic diseases. Furthermore, the study highlights the importance of targeted treatment, including genetic counseling, to improve patient outcomes. The observed association between autoimmunity and immune deficiency reinforces the importance of molecular testing in elucidating the causes of previously idiopathic rheumatic diseases, contributing to improved patient care and quality of life.
The Interaction of the Endocannabinoid Anandamide and Paracannabinoid Lysophosphatidylinositol during Cell Death Induction in Human Breast Cancer Cells
Authors: Akimov Mikhail G. , Gretskaya Natalia M. , Gorbacheva Evgenia I. , Khadour Nisreen , Chernavskaya Valeria S. , Sherstyanykh Galina D. , Kovaleko Tatiana F. , Fomina-Ageeva Elena V. , Bezuglov Vladimir V.
Published: February 2024

Endocannabinoid anandamide (AEA) and paracannabinoid lysophosphatidylinositol (LPI) play a significant role in cancer cell proliferation regulation. While anandamide inhibits the proliferation of cancer cells, LPI is known as a cancer stimulant. Despite the known endocannabinoid receptor crosstalk and simultaneous presence in the cancer microenvironment of both molecules, their combined activity has never been studied. We evaluated the effect of LPI on the AEA activity in six human breast cancer cell lines of different carcinogenicity (MCF-10A, MCF-7, BT-474, BT-20, SK-BR-3, MDA-MB-231) using resazurin and LDH tests after a 72 h incubation. AEA exerted both anti-proliferative and cytotoxic activity with EC50 in the range from 31 to 80 µM. LPI did not significantly affect the cell viability. Depending on the cell line, the response to the LPI–AEA combination varied from a decrease in AEA cytotoxicity to an increase in it. Based on the inhibitor analysis of the endocannabinoid receptor panel, we showed that for the former effect, an active GPR18 receptor was required and for the latter, an active CB2 receptor. The data obtained for the first time are important for the understanding the manner by which endocannabinoid receptor ligands acting simultaneously can modulate cancer growth at different stages.
The Association of ADAMTS7 Gene Polymorphisms with the Risk of Coronary Artery Disease Occurrence and Cardiovascular Survival in the Polish Population: A Case-Control and a Prospective Cohort Study
Authors: Iwanicka Joanna , Balcerzyk-Matić Anna , Iwanicki Tomasz , Mizia-Stec Katarzyna , Bańka Paweł , Filipecki Artur , Gawron Katarzyna , Jarosz Alicja , Nowak Tomasz , Krauze Jolanta , Niemiec Paweł
Published: February 2024

The aim of this study was to investigate whether the polymorphisms of the ADAMTS7 gene affect the risk of occurrence and mortality due to CAD. The study group included 231 patients diagnosed with CAD and 240 control blood donors. The genotyping of specified polymorphisms, i.e., rs1994016, rs3825807, and rs7173743, was performed using the TaqMan-PCR. We found that the C allele carriers of the rs1994016 and A allele carriers of the rs3825807 polymorphisms increased the risk of CAD, respectively: OR = 1.72, p = 0.036; OR = 1.64, p = 0.04. Moreover, we studied the biological interactions of specified variants, i.e., rs3825807, rs1994016, and rs7173743, and previously approved risk factors of CAD. We demonstrated here that selected polymorphisms of ADAMTS7 increased the risk of CAD altogether with abnormalities of total cholesterol and LDL concentrations in serum. Although survival analyses did not reveal statistical significance, we observed a trend for the AA genotype of the rs3825807 ADAMTS7, which may predispose to death due to CAD in a 5-year follow-up. In conclusion, the ADAMTS7 polymorphisms investigated in this study may increase the risk of occurrence and/or death due to CAD in the Polish population.
Harvesting the Power of Green Synthesis: Gold Nanoparticles Tailored for Prostate Cancer Therapy
Authors: Oliveira Marco , Sousa André , Sá Sara , Soares Sílvia , Pereira Ana Cláudia , Rocha Ana Catarina , Pais Patrick , Ferreira Diogo , Almeida Cátia , Luís Carla , Lima Cláudio , Almeida Fábio , Gestoso Álvaro , Duarte Miguel-Correa , Barata Pedro , Martins-Mendes Daniela , Baylina Pilar , Pereira Carla F. , Fernandes Rúben
Published: February 2024

Biosynthetic gold nanoparticles (bAuNPs) present a promising avenue for enhancing bio-compatibility and offering an economically and environmentally responsible alternative to traditional production methods, achieved through a reduction in the use of hazardous chemicals. While the potential of bAuNPs as anticancer agents has been explored, there is a limited body of research focusing on the crucial physicochemical conditions influencing bAuNP production. In this study, we aim to identify the optimal growth phase of Pseudomonas aeruginosa cultures that maximizes the redox potential and coordinates the formation of bAuNPs with increased efficiency. The investigation employs 2,6-dichlorophenolindophenol (DCIP) as a redox indicator. Simultaneously, we explore the impact of temperature, pH, and incubation duration on the biosynthesis of bAuNPs, with a specific emphasis on their potential application as antitumor agents. Characterization of the resulting bAuNPs is conducted using ATR-FT-IR, TEM, and UV-Vis spectroscopy. To gain insights into the anticancer potential of bAuNPs, an experimental model is employed, utilizing both non-neoplastic (HPEpiC) and neoplastic (PC3) epithelial cell lines. Notably, P. aeruginosa cultures at 9 h/OD600 = 1, combined with biosynthesis at pH 9.0 for 24 h at 58 °C, produce bAuNPs that exhibit smaller, more spherical, and less aggregated characteristics. Crucially, these nanoparticles demonstrate negligible effects on HPEpiC cells while significantly impacting PC3 cells, resulting in reduced viability, migration, and lower IL-6 levels. This research lays the groundwork for the development of more specialized, economical, and ecologically friendly treatment modalities.
Extreme Reconfiguration of Plastid Genomes in Papaveraceae: Rearrangements, Gene Loss, Pseudogenization, IR Expansion, and Repeats
Authors: Cao Jialiang , Wang Hongwei , Cao Yanan , Kan Shenglong , Li Jiamei , Liu Yanyan
Published: February 2024

The plastid genomes (plastomes) of angiosperms are typically highly conserved, with extreme reconfiguration being uncommon, although reports of such events have emerged in some lineages. In this study, we conducted a comprehensive comparison of the complete plastomes from twenty-two species, covering seventeen genera from three subfamilies (Fumarioideae, Hypecooideae, and Papaveroideae) of Papaveraceae. Our results revealed a high level of variability in the plastid genome size of Papaveraceae, ranging from 151,864 bp to 219,144 bp in length, which might be triggered by the expansion of the IR region and a large number of repeat sequences. Moreover, we detected numerous large-scale rearrangements, primarily occurring in the plastomes of Fumarioideae and Hypecooideae. Frequent gene loss or pseudogenization were also observed for ndhs, accD, clpP, infA, rpl2, rpl20, rpl32, rps16, and several tRNA genes, particularly in Fumarioideae and Hypecooideae, which might be associated with the structural variation in their plastomes. Furthermore, we found that the plastomes of Fumarioideae exhibited a higher GC content and more repeat sequences than those of Papaveroideae. Our results showed that Papaveroideae generally displayed a relatively conserved plastome, with the exception of Eomecon chionantha, while Fumarioideae and Hypecooideae typically harbored highly reconfigurable plastomes, showing high variability in the genome size, gene content, and gene order. This study provides insights into the plastome evolution of Papaveraceae and may contribute to the development of effective molecular markers.
Comparative Mitogenome Analyses of Fifteen Ramshorn Snails and Insights into the Phylogeny of Planorbidae (Gastropoda: Hygrophila)
Authors: Tao Kexin , Gao Yue , Yin Haofei , Liang Qichao , Yang Qianqian , Yu Xiaoping
Published: February 2024

Ramshorn snails from the family Planorbidae are important freshwater snails due to their low trophic level, and some of them act as intermediate hosts for zoonotic trematodes. There are about 250 species from 40 genera of Planorbidae, but only 14 species from 5 genera (Anisus, Biomphalaria, Bulinus, Gyraulus, and Planorbella) have sequenced complete mitochondrial genomes (mitogenomes). In this study, we sequenced and assembled a high-quality mitogenome of a ramshorn snail, Polypylis sp. TS-2018, which represented the first mitogenome of the genus. The mitogenome of Polypylis sp. TS-2018 is 13,749 bp in length, which is shorter than that of most gastropods. It contains 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, and 2 ribosomal RNA (rRNA). We compared mitogenome characteristics, selection pressure, and gene rearrangement among all of the available mitogenomes of ramshorn snails. We found that the nonsynonymous and synonymous substitution rates (Ka/Ks) of most PCGs indicated purifying and negative selection, except for atp8 of Anisus, Biomphalaria, and Gyraulus, which indicated positive selection. We observed that transpositions and reverse transpositions occurred on 10 tRNAs and rrnS, which resulted in six gene arrangement types. We reconstructed the phylogenetic trees using the sequences of PCGs and rRNAs and strongly supported the monophyly of each genus, as well as three tribes in Planorbidae. Both the gene rearrangement and phylogenetic results suggested that Polypylis had a close relationship with Anisus and Gyraulus, while Bulinus was the sister group to all of the other genera. Our results provide useful data for further investigation of species identification, population genetics, and phylogenetics among ramshorn snails.