bluuebunny/crossref_metadata_2025 huggingface dataset:
Children with Autism Spectrum Disorder Exhibit Elevated Physical Activity and Reduced Sedentary Behavior
Authors: Alhowikan Abdulrahman M. , Elamin Nadra E. , Aldayel Sarah S. , AlSiddiqi Sara A. , Alrowais Fai S. , Hassan Wail M. , El-Ansary Afaf , Alghamdi Farah Ali , AL-Ayadhi Laila Y.
Published: November 2023

According to previous research, individuals with autism spectrum disorder (ASD) have lower levels of physical activity than their typically developed (TD) counterparts. There have been conflicting reports about physical activity (PA) levels in people with ASD. Given the conflicting evidence, further investigation is required. We believe that evaluating PA in individuals with ASD is critical in order to offer PA intervention plans aiming at increasing their health-related physical fitness on a daily, systematic, and individualized basis. In the current study, an ActiGraph monitor (GT3X+) was used to accurately measure PA and sedentary activity in 21 children with autism aged 6.43 ± 2.29 years and 30 TD children aged 7.2 ± 3.14 years. Our data indicated that while the light and moderate activity counts were not significantly different between the two groups, the vigorous activity was significantly higher in ASD compared to TD. This finding was attributed to ASD characteristic stereotypy and self-stimulating behaviors. The significantly higher vigorous PA is discussed in relation to altered neurochemistry, oxidative stress, and neuroinflammation as etiological mechanisms in ASD. This research provides a better understanding of the status of PA participation in individuals with ASD.
Psychophysiological and Neurophysiological Correlates of Dropping Objects from Hands in Carpal Tunnel Syndrome
Authors: Isoardo Gianluca , Rota Eugenia , Ciullo Stefano , Titolo Paolo , Matteoni Enrico , Stura Ilaria , Calvo Andrea , Fontana Elena , Battiston Bruno , Migliaretti Giuseppe , Ardito Rita B. , Adenzato Mauro
Published: November 2023

Background: Dropping objects from hands (DOH) is a common symptom of carpal tunnel syndrome (CTS). We evaluated the clinical, neurophysiological, and psychophysiological features of 120 CTS patients to elucidate the DOH pathophysiology. Forty-nine healthy controls were included. Methods: In the patients, the Boston Carpal Tunnel Questionnaire (BCTQ), the Douleur Neuropathique 4 questions (DN4), and a numeric rating scale for pain (NRS) were evaluated. In patients and controls, we evaluated bilateral median and ulnar motor and sensory nerve conduction studies, cutaneous silent period and cutaneomuscular reflexes (CMR) of the abductor pollicis brevis, cold-detection threshold (CDT) and heat-pain detection threshold (HPT) at the index, little finger, and dorsum of the hand, and vibratory detection threshold at the index and little finger by quantitative sensory testing. Results: CTS with DOH had higher BCTQ, DN4 and NRS, lower median sensory action potential, longer CMR duration, lower CDT and higher HPT at all tested sites than controls and CTS without DOH. Predictive features for DOH were abnormal CDT and HPT at the right index and dorsum (OR: 3.88, p: 0.03) or at the little finger (OR: 3.27, p: 0.04) and a DN4 higher than 4 (OR: 2.16, p < 0.0001). Conclusions: Thermal hypoesthesia in median and extra-median innervated territories and neuropathic pain are predictive of DOH in CTS.
Cancer Epigenetic Biomarkers
Authors: Coppedè Fabio
Published: November 2023

This series of nine articles (six original articles, three reviews) is presented by international experts in cancer epigenetics [...]
The Olivine Horizon of the Layered Monchegorsk Pluton (Kola Region, Russia): Additional Magma Injection Based on Integrated Geological and Geochronological Data
Authors: Chashchin Victor , Sergeev Sergey
Published: November 2023

The paper presents the first SIMS SHRIMP U-Pb data for zircon from an olivine horizon within the Nyud intrusion of the ore-bearing layered Monchegorsk pluton (Monchepluton) in the Kola Region, Russia. A 100–150 m-thick olivine horizon occurs nearly horizontally between the melanocratic and mesocratic norite of the Nyud intrusion, which disturbs its normal cumulus stratigraphic sequence. In addition, the pyroxene-plagioclase hornfelses are present at the upper contact with the olivine horizon. Twenty-three zircon grains were extracted from the large-volume olivine plagio-orthopyroxenite sample and clustered into two populations. The first population of magmatic zircon (n = 8) has a concordant and weighted average 207Pb/206Pb age of 2484.3 ± 5.6 Ma, which characterizes the formation time of the olivine horizon rocks. This serves as evidence of the olivine horizon that forms as a result of additional magma injection, which does not contradict the geological data. The 207Pb/206Pb age of single-grain zircon is 2414 ± 25 Ma, which indicates the time of postmagmatic transformations. The second population of zircon (n = 16) has a concordant and weighted average U-Pb age of 2700.6 ± 4.6 Ma, which indicates zircon absorption by olivine horizon magma probably from the rocks of the Archean greenstone belt.
The Equilibria of Triterpene Sapogenins–Phosphatidylcholine in Monolayers at the Air/Water Interface
Authors: Karwowska Katarzyna , Urbaniak Wiesław , Petelska Aneta D.
Published: November 2023

Sapogenins are the non-sugar parts of saponins (aglycones), high-molecular-weight glycosides linked to one or more sugar side chains. This group of compounds presents many properties, e.g., the potent properties of reducing surface tension and foaming properties, as evidenced by the amphipathic nature of these substances. They are used in the cosmetics industry, the washing and detergent industry, and the food industry. In addition, they have many healing properties. They lower blood cholesterol but are also used to synthesize steroid drugs or hormones. As reported in the literature, saponins also show antitumor activity, leading to cell cycle inhibition and apoptosis of various neoplastic cells. In this study, the influence of two sapogenins: asiatic acid (AA) and oleanolic acid (OA), on the properties of monolayers made of phosphatidylcholine (DPPC) was investigated. The method used in these studies was the Langmuir method with Brewster angle microscopy. The interactions between the tested compounds in mixed monolayers were described. Using mathematical equations, we established that oleanolic acid and asiatic acid formed complexes with DPPC at 1:1 ratios, characterized by high stability constants. We derived the parameters characterizing the formed complexes and described the phase transitions that occur during the formation of pure and mixed monolayers.
A Novel Variant in TPM3 Causing Muscle Weakness and Concomitant Hypercontractile Phenotype
Authors: Robaszkiewicz Katarzyna , Siatkowska Małgorzata , Wadman Renske I. , Kamsteeg Erik-Jan , Chen Zhiyong , Merve Ashirwad , Parton Matthew , Bugiardini Enrico , de Bie Charlotte , Moraczewska Joanna
Published: November 2023

A novel variant of unknown significance c.8A > G (p.Glu3Gly) in TPM3 was detected in two unrelated families. TPM3 encodes the transcript variant Tpm3.12 (NM_152263.4), the tropomyosin isoform specifically expressed in slow skeletal muscle fibers. The patients presented with slowly progressive muscle weakness associated with Achilles tendon contractures of early childhood onset. Histopathology revealed features consistent with a nemaline rod myopathy. Biochemical in vitro assays performed with reconstituted thin filaments revealed defects in the assembly of the thin filament and regulation of actin–myosin interactions. The substitution p.Glu3Gly increased polymerization of Tpm3.12, but did not significantly change its affinity to actin alone. Affinity of Tpm3.12 to actin in the presence of troponin ± Ca2+ was decreased by the mutation, which was due to reduced interactions with troponin. Altered molecular interactions affected Ca2+-dependent regulation of the thin filament interactions with myosin, resulting in increased Ca2+ sensitivity and decreased relaxation of the actin-activated myosin ATPase activity. The hypercontractile molecular phenotype probably explains the distal joint contractions observed in the patients, but additional research is needed to explain the relatively mild severity of the contractures. The slowly progressive muscle weakness is most likely caused by the lack of relaxation and prolonged contractions which cause muscle wasting. This work provides evidence for the pathogenicity of the TPM3 c.8A > G variant, which allows for its classification as (likely) pathogenic.
GPX3 Variant Genotype Affects the Risk of Developing Severe Forms of COVID-19
Authors: Markovic Marko , Ranin Jovan , Bukumiric Zoran , Jerotic Djurdja , Savic-Radojevic Ana , Pljesa-Ercegovac Marija , Djukic Tatjana , Ercegovac Marko , Asanin Milika , Milosevic Ivana , Stevanovic Goran , Simic Tatjana , Coric Vesna , Matic Marija
Published: November 2023

In SARS-CoV-2 infection, excessive activation of the immune system intensively increases reactive oxygen species levels, causing harmful hyperinflammatory and oxidative state cumulative effects which may contribute to COVID-19 severity. Therefore, we assumed that antioxidant genetic profile, independently and complemented with laboratory markers, modulates COVID-19 severity. The study included 265 COVID-19 patients. Polymorphism of GSTM1, GSTT1, Nrf2 rs6721961, GSTM3 rs1332018, GPX3 rs8177412, GSTP1 rs1695, GSTO1 rs4925, GSTO2 rs156697, SOD2 rs4880 and GPX1 rs1050450 genes was determined with appropriate PCR-based methods. Inflammation (interleukin-6, CRP, fibrinogen, ferritin) and organ damage (urea, creatinine, transaminases and LDH) markers, complete blood count and coagulation status (d-dimer, fibrinogen) were measured. We found significant association for COVID-19 progression for patients with lymphocytes below 1.0 × 109/L (OR = 2.97, p = 0.002). Increased IL-6 and CRP were also associated with disease progression (OR = 8.52, p = 0.001, and OR = 10.97, p < 0.001, respectively), as well as elevated plasma AST and LDH (OR = 2.25, p = 0.021, and OR = 4.76, p < 0.001, respectively). Of all the examined polymorphisms, we found significant association with the risk of developing severe forms of COVID-19 for GPX3 rs8177412 variant genotype (OR = 2.42, p = 0.032). This finding could be of particular importance in the future, complementing other diagnostic tools for prediction of COVID-19 disease course.
Non-Invasive Physical Plasma Reduces the Inflammatory Response in Microbially Prestimulated Human Gingival Fibroblasts
Authors: Eggers Benedikt , Stope Matthias Bernhard , Marciniak Jana , Mustea Alexander , Eick Sigrun , Deschner James , Nokhbehsaim Marjan , Kramer Franz-Josef
Published: November 2023

Non-invasive physical plasma (NIPP), an electrically conductive gas, is playing an increasingly important role in medicine due to its antimicrobial and regenerative properties. However, NIPP is not yet well established in dentistry, although it has promising potential, especially for periodontological applications. The aim of the present study was to investigate the effect of NIPP on a commercially available human gingival fibroblast (HGF) cell line and primary HGFs in the presence of periodontitis-associated bacteria. First, primary HGFs from eight patients were characterised by immunofluorescence, and cell numbers were examined by an automatic cell counter over 5 days. Then, HGFs that were preincubated with Fusobacterium nucleatum (F.n.) were treated with NIPP. Afterwards, the IL-6 and IL-8 levels in the cell supernatants were determined by ELISA. In HGFs, F.n. caused a significant increase in IL-6 and IL-8, and this F.n.-induced upregulation of both cytokines was counteracted by NIPP, suggesting a beneficial effect of physical plasma on periodontal cells in a microbial environment. The application of NIPP in periodontal therapy could therefore represent a novel and promising strategy and deserves further investigation.
The Value of ER∝ in the Prognosis of GH- and PRL-Secreting PitNETs: Clinicopathological Correlations
Authors: Dumitriu-Stan Roxana-Ioana , Burcea Iulia-Florentina , Nastase Valeria Nicoleta , Ceaușu Raluca Amalia , Dumitrascu Anda , Cocosila Laurentiu Catalin , Bastian Alexandra , Zurac Sabina , Raica Marius , Poiana Catalina
Published: November 2023

Pituitary neuroendocrine tumors (PitNETs) are divided into multiple histological subtypes, which determine their clinical and biological variable behavior. Despite their benign evolution, in some cases, prolactin (PRL) and growth hormone (GH)-secreting PitNETs may have aggressive behavior. In this study, we investigated the potential predictive role of ER∝, alongside the clinicopathological classification of PitNETs (tumor diameter, tumor type, and tumor grade). A retrospective study was conducted with 32 consecutive cases of PRL- and mixed GH- and PRL-secreting PitNETs (5 patients with prolactinomas and 27 with acromegaly, among them, 7 patients with GH- and PRL- co-secretion) who underwent transsphenoidal intervention. Tumor specimens were histologically and immunohistochemical examined: anterior pituitary hormones, ki-67 labeling index, CAM 5.2, and ER∝; ER∝ expression was correlated with basal PRL levels at diagnosis (rho = 0.60, p < 0.01) and postoperative PRL levels (rho = 0.58, p < 0.001). In our study, the ER∝ intensity score was lower in female patients. Postoperative maximal tumor diameter correlated with Knosp grade (p = 0.02); CAM 5.2 pattern (densely/sparsely granulated/mixed densely and sparsely granulated) was correlated with postoperative PRL level (p = 0.002), and with ki-67 (p < 0.001). The IGF1 level at diagnosis was correlated with the postoperative GH nadir value in the oral glucose tolerance test (OGTT) (rho = 0.52, p < 0.05). Also, basal PRL level at diagnosis was correlated with postoperative tumor diameter (p = 0.63, p < 0.001). At univariate logistic regression, GH nadir in OGTT test at diagnostic, IGF1, gender, and invasion were independent predictors of remission for mixed GH- and PRL-secreting Pit-NETs; ER∝ can be used as a prognostic marker and loss of ER∝ expression should be considered a sign of lower differentiation and a likely indicator of poor prognosis. A sex-related difference can be considered in the evolution and prognosis of these tumors, but further studies are needed to confirm this hypothesis.
Exploring Reliable and Efficient Plasmonic Nanopatterning for Surface- and Tip-Enhanced Raman Spectroscopies
Authors: Sasso Antonio , Capaccio Angela , Rusciano Giulia
Published: November 2023

Surface-enhanced Raman scattering (SERS) is of growing interest for a wide range of applications, especially for biomedical analysis, thanks to its sensitivity, specificity, and multiplexing capabilities. A crucial role for successful applications of SERS is played by the development of reproducible, efficient, and facile procedures for the fabrication of metal nanostructures (SERS substrates). Even more challenging is to extend the fabrication techniques of plasmonic nano-textures to atomic force microscope (AFM) probes to carry out tip-enhanced Raman spectroscopy (TERS) experiments, in which spatial resolution below the diffraction limit is added to the peculiarities of SERS. In this short review, we describe recent studies performed by our group during the last ten years in which novel nanofabrication techniques have been successfully applied to SERS and TERS experiments for studying bio-systems and molecular species of environmental interest.
C-C Motif Chemokine Ligand 5 (CCL5) Promotes Irradiation-Evoked Osteoclastogenesis
Authors: Wang Jing , Zhao Fanyu , Xu Linshan , Wang Jianping , Zhai Jianglong , Ren Li , Zhu Guoying
Published: November 2023

The imbalance that occurs in bone remodeling induced by irradiation (IR) is the disruption of the balance between bone formation and bone resorption. In this study, primary osteocytes (OCYs) of femoral and tibial origin were cultured and irradiated. It was observed that irradiated OCY showed extensive DNA damage, which led to the initiation of a typical phenotype of cellular senescence, including the secretion of senescence-associated secretory phenotype (SASP), especially the C-C motif chemokine ligand 5 (CCL5). In order to explore the regulation of osteoclastogenic potential by IR-induced senescent OCYs exocytosis factor CCL5, the conditioned medium (CM) of OCYs was co-cultured with RAW264.7 precursor cells. It was observed that in the irradiated OCY co-cultured group, the migration potential increased compared with the vehicle culture group, accompanied by an enhancement of typical mature OCs; the expression of the specific function of enzyme tartrate-resistant acid phosphatase (TRAP) increased; and the bone-destructive function was enhanced. However, a neutralizing antibody to CCL5 could reverse the extra-activation of osteoclastogenesis. Accordingly, the overexpression of p-STAT3 in irradiated OCY was accompanied by CCL5. It was concluded that CCL5 is a potential key molecule and the interventions targeting CCL5 could be a potential strategy for inhibiting osteoclastogenesis and restoring bone remodeling.
Studying the Human Microbiota: Advances in Understanding the Fundamentals, Origin, and Evolution of Biological Timekeeping
Authors: Siebieszuk Adam , Sejbuk Monika , Witkowska Anna Maria
Published: November 2023

The recently observed circadian oscillations of the intestinal microbiota underscore the profound nature of the human–microbiome relationship and its importance for health. Together with the discovery of circadian clocks in non-photosynthetic gut bacteria and circadian rhythms in anucleated cells, these findings have indicated the possibility that virtually all microorganisms may possess functional biological clocks. However, they have also raised many essential questions concerning the fundamentals of biological timekeeping, its evolution, and its origin. This narrative review provides a comprehensive overview of the recent literature in molecular chronobiology, aiming to bring together the latest evidence on the structure and mechanisms driving microbial biological clocks while pointing to potential applications of this knowledge in medicine. Moreover, it discusses the latest hypotheses regarding the evolution of timing mechanisms and describes the functions of peroxiredoxins in cells and their contribution to the cellular clockwork. The diversity of biological clocks among various human-associated microorganisms and the role of transcriptional and post-translational timekeeping mechanisms are also addressed. Finally, recent evidence on metabolic oscillators and host–microbiome communication is presented.
Serum Reactive Antibodies against the N-Methyl-D-Aspartate Receptor NR2 Subunit—Could They Act as Potential Biomarkers?
Authors: Hadjiagapiou Maria S. , Krashias George , Christodoulou Christina , Pantzaris Marios , Lambrianides Anastasia
Published: November 2023

Synaptic dysfunction and disrupted communication between neuronal and glial cells play an essential role in the underlying mechanisms of multiple sclerosis (MS). Earlier studies have revealed the importance of glutamate receptors, particularly the N-methyl-D-aspartate (NMDA) receptor, in excitotoxicity, leading to abnormal synaptic transmission and damage of neurons. Our study aimed to determine whether antibodies to the NR2 subunit of NMDAR are detected in MS patients and evaluate the correlation between antibody presence and clinical outcome. Furthermore, our focus extended to examine a possible link between NR2 reactivity and anti-coagulant antibody levels as pro-inflammatory molecules associated with MS. A cross-sectional study was carried out, including 95 patients with MS and 61 age- and gender-matched healthy controls (HCs). The enzyme-linked immunosorbent assay was used to detect anti-NR2 antibodies in serum samples of participants along with IgG antibodies against factor (F)VIIa, thrombin, prothrombin, FXa, and plasmin. According to our results, significantly elevated levels of anti-NR2 antibodies were detected in MS patients compared to HCs (p < 0.05), and this holds true when we compared the Relapsing-Remitting MS course with HCs (p < 0.05). A monotonically increasing correlation was found between NR2 seropositivity and advanced disability (rs = 0.30; p < 0.01), anti-NR2 antibodies and disease worsening (rs = 0.24; p < 0.05), as well as between antibody activity against NR2 and thrombin (rs = 0.33; p < 0.01). The presence of anti-NR2 antibodies in MS patients was less associated with anti-plasmin IgG antibodies [OR:0.96 (95%CI: 0.92–0.99); p < 0.05]; however, such an association was not demonstrated when analyzing only RRMS patients. In view of our findings, NR2-reactive antibodies may play, paving the way for further research into their potential as biomarkers and therapeutic targets in MS.
Lethal and Sub-Lethal Effects of Organic-Production-Approved Insecticides and Fungicides on the Predator Macrolophus pygmaeus (Rambur) (Hemiptera: Miridae)
Authors: Betsi Petri C. , Perdikis Dionysios Ch
Published: November 2023

In this study, the effects of paraffin oil, copper hydroxide, copper oxychloride, wettable sulfur, Beauveria bassiana and deltamethrin, as a toxic reference treatment, on the survival and predation rate of M. pygmaeus were investigated. In each treatment, the prey were classified as slightly, partially or fully consumed. The mortality rate after contact exposure was high (66.6%) when nymphs were treated with copper hydroxide but much lower after residual exposure (6.6%). B. bassiana caused 53.3% and 46.6% mortality via contact and residual exposure, respectively. The total prey consumption was significantly lower in the pyrethroid reference treatment control and B. bassiana treatments. The highest percentage of slightly consumed prey was recorded in the toxic reference and B. bassiana treatments, that of partially consumed prey in the copper hydroxide treatment and, finally, that of fully consumed prey in the paraffin oil treatment. Therefore, assessing the sub-lethal effects by separating the prey killed into slightly, partially and fully consumed is a sensitive approach to detect impacts which otherwise may remain unnoticed. The results provide information for the most appropriate use of M. pygmaeus in IPM programs and introduce more sensitive approaches in the detection of side-effects of pesticides on M. pygmaeus and other hemipteran predators.
Efficacy of Omega-3 Intake in Managing Dry Eye Disease: A Systematic Review and Meta-Analysis of Randomized Controlled Trials
Authors: Wang Wei-Xiang , Ko Mei-Lan
Published: November 2023

To explore the efficacy of omega-3 fatty acids (FAs) on patients suffering from dry eye disease (DED), a complex inflammatory condition, we reviewed data from PubMed, Embase, ClinicalTrials.gov, Web of Science, and Cochrane CENTRAL in the past 10 years (2013 to 2023). These sources provided randomized clinical trials (RCTs) that examined the efficacy of omega-3 FAs on DED patients with accessible pre- and post-intervention data, excluding trials with overlapping participants, without omega-3 supplementation, or those lacking placebo control or quantitative assessments. Two independent reviewers extracted data related to dry eye symptom scores, tear break-up time (TBUT), Schirmer’s tests, osmolarity, and corneal fluorescein staining (CFS), and the results were analyzed by Comprehensive Meta-Analysis software version 4. We incorporated 19 related RCTs assessed by the Cochrane Risk of Bias tool, encompassing 4246 DED patients with various etiologies. Patients given omega-3 treatment demonstrated more significant improvements in dry eye symptoms (Hedges’ g = −1.047; p < 0.001), TBUT [standardized mean difference (SMD) = −0.939; p < 0.001], scores from the Schirmer test (SMD = −0.372; p < 0.001), CFS (SMD = −0.299; p = 0.037), and osmolarity (SMD = −0.721; p < 0.001) compared to those on a placebo regimen. In the meta-regression analysis of DED symptoms, the daily dose of omega-3 (coefficient = −0.0005, p = 0.002), duration of omega-3 intake (coefficient = −0.1399, p = 0.021), and percentage of eicosapentaenoic acid (EPA) (coefficient = −0.0154, p < 0.001) exhibited a significant positive correlation with a reduction in dry eye symptom scores. Apart from CFS, similar trends were noted in TBUT, Schirmer tests, and osmolarity scores. Based on the evidence, omega-3 FAs effectively reduce DED symptoms, especially in high doses, for a long duration, and with increased EPA levels. However, given the heterogeneity in study results and diverse patient characteristics, caution is needed in generalizing these findings. In conclusion, omega-3 FA supplementation is still recommended for DED management in clinical settings.
Microwaved-Assisted Synthesis of Starch-Based Biopolymer Membranes for Novel Green Electrochemical Energy Storage Devices
Authors: Jeżowski Paweł , Menzel Jakub , Baranowska Hanna Maria , Kowalczewski Przemysław Łukasz
Published: November 2023

The investigated starch biopolymer membrane was found to be a sustainable alternative to currently reported and used separators due to its properties, which were evaluated using physicochemical characterization. The molecular dynamics of the biomembrane were analyzed using low-field nuclear magnetic resonance (LF NMR) as well as Raman and infrared spectroscopy, which proved that the chemical composition of the obtained membrane did not degrade during microwave-assisted polymerization. Easily and cheaply prepared through microwave-assisted polymerization, the starch membrane was successfully used as a biodegradable membrane separating the positive and negative electrodes in electric double-layer capacitors (EDLCs). The obtained results for the electrochemical characterization via cyclic voltammetry (CV), galvanostatic charge with potential limitation (GCPL), and electrochemical impedance spectroscopy (EIS) show a capacitance of 30 F g−1 and a resistance of 2 Ohms; moreover, the longevity of the EDLC during electrochemical floating exceeded more than 200 h or a cyclic ability of 50,000 cycles. Furthermore, due to the flexibility of the membrane, it can be easily used in novel, flexible energy storage systems. This proves that this novel biomembrane can be a significant step toward ecologically friendly energy storage devices and could be considered a cheaper alternative to currently used materials, which cannot easily biodegrade over time in comparison to biopolymers.
The Effects of Enzymes, Species, and Storage of Raw Material on Physicochemical Properties of Protein Hydrolysates from Whitefish Heads
Authors: Remme Jannicke Fugledal , Korsnes Sigurd , Steen Stine , Durand Rachel , Kvangarsnes Kristine , Stangeland Janne
Published: November 2023

The rest raw materials of whitefish have great potential for increased utilisation and value creation. Whitefish heads have a high protein content and should be considered a healthy protein source for the growing population’s demands for sustainable protein. In this study, the heads of four different species of whitefish were processed via enzymatic hydrolysis, namely cod (Gadus morhua), cusk (Brosme bromse), haddock (Melanogrammus aeglefinus), and saithe (Pollachius virens), using three commercially available enzymes. Trials were conducted after 0, 3, and 6 months of the frozen storage of heads. A proximate analysis, molecular weight distribution, and protein solubility were evaluated for each of the products. The results show that, although the enzymatic hydrolysis of rest raw materials from different species of whitefish yielded products of slightly different characteristics, this process is viable for the production of high-quality protein from cod, cusk, haddock, and saithe heads. Six months of frozen storage of heads had a minimal effect on the yield and proximate composition of hydrolysates.
Copper Nanoparticles Confined in a Silica Nanochannel Film for the Electrochemical Detection of Nitrate Ions in Water Samples
Authors: Li Dewang , Xu Shuai , Jin Haiyan , Wang Jinqing , Yan Fei
Published: November 2023

The nitrate ion (NO3−) is a typical pollutant in environmental samples, posing a threat to the aquatic ecosystem and human health. Therefore, rapid and accurate detection of NO3− is crucial for both the aquatic sciences and government regulations. Here we report the fabrication of an amino-functionalized, vertically ordered mesoporous silica film (NH2-VMSF) confining localized copper nanoparticles (CuNPs) for the electrochemical detection of NO3−. NH2-VMSF-carrying amino groups possess an ordered perpendicular nanochannel structure and ultrasmall nanopores, enabling the confined growth of CuNPs through the electrodeposition method. The resulting CuNPs/NH2-VMSF-modified indium tin oxide (ITO) electrode (CuNPs/NH2-VMSF/ITO) combines the electrocatalytic reduction ability of CuNPs and the electrostatic attraction capacity of NH2-VMSF towards NO3−. Thus, it is a rapid and sensitive electrochemical method for the determination of NO3− with a wide linear detection range of 5.0–1000 μM and a low detection limit of 2.3 μM. Direct electrochemical detection of NO3− in water samples (tap water, lake water, seawater, and rainwater) with acceptable recoveries ranging from 97.8% to 109% was performed, demonstrating that the proposed CuNPs/NH2-VMSF/ITO sensor has excellent reproducibility, regeneration, and anti-interference abilities.
National Nutrition Surveys Applying Dietary Records or 24-h Dietary Recalls with Questionnaires: A Scoping Review
Authors: Okada Emiko , Nakade Makiko , Hanzawa Fumiaki , Murakami Kentaro , Matsumoto Mai , Sasaki Satoshi , Takimoto Hidemi
Published: November 2023

Development of an accurate and efficient dietary method is required for national nutrition surveys. Some countries conduct dietary surveys and combine 24-h dietary records or 24-h dietary recalls with dietary questionnaires. This scoping review aimed to summarize studies that used results from national surveys that combined detailed dietary surveys (dietary records or 24-h dietary recall) and dietary questionnaires and identify the purpose of combining the two methods. The PubMed database and manual searches were used for the literature review. We extracted 58 articles from 16 national nutrition surveys from 14 countries. Most studies used 24-h dietary recall for detailed dietary surveys and the food frequency questionnaire (FFQ) or food propensity questionnaire (FPQ) for questionnaire surveys. Among 37 studies from eight countries, the purpose of combining the two dietary survey methods was to estimate energy and nutrient intakes from detailed dietary surveys and habitual food intake from questionnaires. These findings are useful as a reference when introducing new dietary survey methods in future national nutrition surveys.
Neurodevelopmental Outcome in Very Preterm Infants Randomised to Receive Two Different Standardised, Concentrated Parenteral Nutrition Regimens
Authors: Morgan Colin , Parry Samantha , Park Julie , Tan Maw
Published: November 2023

We have previously shown that increasing parenteral protein (target: 3.8 versus 2.8 g/kg/d) and energy (12% versus 10% glucose; 3.8 versus 2.8 g/kg/d) intake using a Standardised, Concentrated with Added Macronutrients Parenteral (SCAMP) nutrition regimen ameliorates early head growth failure in very-preterm infants (VPIs). We hypothesised that the SCAMP nutrition regimen would also improve neurodevelopmental outcome. The original double-blind randomised, controlled study (ISRCTN: 76597892) received ethical approval. VPIs were randomised to either start SCAMP or remain on the control regimen. The consent process included neurodevelopmental assessments (Bayley III), all of which were performed (blinded) at 2–3.5 years of corrected gestational age. Bayley III assessments were performed for 38/60 SCAMP survivors and 41/63 control survivors at means of (sd) 29.2 (3.7) and 20.0 (3.9) months, respectively. Motor, cognitive, language, and combined scores were all higher in the SCAMP intervention group, but none of the differences were statistically significant. Nutrient intake and biochemical monitoring data confirmed that protein/energy ratios were maintained in the SCAMP intervention group without increasing the incidence of hyperglycaemia, insulin treatment, or the derangement of plasma mineral/electrolyte levels. This study did not show a statistically significant improvement in neurodevelopmental outcome when administering higher parenteral protein/energy intakes despite optimal energy and mineral intakes.
Antidepressant-like Effect of Oroxylum indicum Seed Extract in Mice Model of Unpredictable Chronic Mild Stress
Authors: Chalermwongkul Chorpeth , Khamphukdee Charinya , Maneenet Juthamart , Daodee Supawadee , Monthakantirat Orawan , Boonyarat Chantana , Chotritthirong Yutthana , Awale Suresh , Kijjoa Anake , Chulikhit Yaowared
Published: November 2023

Major depressive disorder (MDD) is one life-threatening disorder that is prevalent worldwide. The evident etiology of this disease is still poorly understood. Currently, herbal medicine is gaining more interest as an alternative antidepressant. Oroxylum indicum, which is used in traditional medicine and contains a potential antidepressive compound, baicalein, could have an antidepressive property. An in vitro monoamine oxidase-A (MAO-A) inhibitory assay was used to preliminarily screening for the antidepressant effect of O. indicum seed (OIS) extract. Mice were subjected to unpredictable chronic mild stress (UCMS) for 6 weeks, and the daily administration of OIS extract started from week 4. The mechanisms involved in the antidepressive activity were investigated. The OIS extract significantly alleviated anhedonia and despair behaviors in the UCMS-induced mouse model via two possible pathways: (i) it normalized the HPA axis function via the restoration of negative feedback (decreased FKBP5 and increased GR expressions) and the reduction in the glucocorticoid-related negative gene (SGK-1), and (ii) it improved neurogenesis via the escalation of BDNF and CREB expressions in the hippocampus and the frontal cortex. In addition, an HPLC analysis of the OIS extract showed the presence of baicalin, baicalein, and chrysin as major constituents. All of the results obtained from this study emphasize the potential of OIS extract containing baicalin and baicalein as an effective and novel alternative treatment for MDD.
SDFPoseGraphNet: Spatial Deep Feature Pose Graph Network for 2D Hand Pose Estimation
Authors: Salman Sartaj Ahmed , Zakir Ali , Takahashi Hiroki
Published: November 2023

In the field of computer vision, hand pose estimation (HPE) has attracted significant attention from researchers, especially in the fields of human–computer interaction (HCI) and virtual reality (VR). Despite advancements in 2D HPE, challenges persist due to hand dynamics and occlusions. Accurate extraction of hand features, such as edges, textures, and unique patterns, is crucial for enhancing HPE. To address these challenges, we propose SDFPoseGraphNet, a novel framework that combines the strengths of the VGG-19 architecture with spatial attention (SA), enabling a more refined extraction of deep feature maps from hand images. By incorporating the Pose Graph Model (PGM), the network adaptively processes these feature maps to provide tailored pose estimations. First Inference Module (FIM) potentials, alongside adaptively learned parameters, contribute to the PGM’s final pose estimation. The SDFPoseGraphNet, with its end-to-end trainable design, optimizes across all components, ensuring enhanced precision in hand pose estimation. Our proposed model outperforms existing state-of-the-art methods, achieving an average precision of 7.49% against the Convolution Pose Machine (CPM) and 3.84% in comparison to the Adaptive Graphical Model Network (AGMN).
Deep Learning Based Fire Risk Detection on Construction Sites
Authors: Ann Hojune , Koo Ki Young
Published: November 2023

The recent large-scale fire incidents on construction sites in South Korea have highlighted the need for computer vision technology to detect fire risks before an actual occurrence of fire. This study developed a proactive fire risk detection system by detecting the coexistence of an ignition source (sparks) and a combustible material (urethane foam or Styrofoam) using object detection on images from a surveillance camera. Statistical analysis was carried out on fire incidences on construction sites in South Korea to provide insight into the cause of the large-scale fire incidents. Labeling approaches were discussed to improve the performance of the object detectors for sparks and urethane foams. Detecting ignition sources and combustible materials at a distance was discussed in order to improve the performance for long-distance objects. Two candidate deep learning models, Yolov5 and EfficientDet, were compared in their performance. It was found that Yolov5 showed slightly higher mAP performances: Yolov5 models showed mAPs from 87% to 90% and EfficientDet models showed mAPs from 82% to 87%, depending on the complexity of the model. However, Yolov5 showed distinctive advantages over EfficientDet in terms of easiness and speed of learning.
Frontier Research on Low-Resource Speech Recognition Technology
Authors: Slam Wushour , Li Yanan , Urouvas Nurmamet
Published: November 2023

With the development of continuous speech recognition technology, users have put forward higher requirements in terms of speech recognition accuracy. Low-resource speech recognition, as a typical speech recognition technology under restricted conditions, has become a research hotspot nowadays because of its low recognition rate and great application value. Under the premise of low-resource speech recognition technology, this paper reviews the research status of feature extraction and acoustic models, and conducts research on resource expansion. Especially in terms of the technical challenges faced by this technology, solutions are proposed, and future research directions are prospected.
Rapid and Efficient NO2 Sensing Performance of TeO2 Nanowires
Authors: Shen Yunkun , Wang Kaili , Liu Hao , Chen Liping , Jin Zhihan , Yan Shancheng
Published: November 2023

Gas sensors play a pivotal role in environmental monitoring, with NO2 sensors standing out due to their exceptional selectivity and sensitivity. Yet, a prevalent challenge remains: the prolonged recovery time of many sensors, often spanning hundreds of seconds, compromises efficiency and undermines the precision of continuous detection. This paper introduces an efficient NO2 sensor using TeO2 nanowires, offering significantly reduced recovery times. The TeO2 nanowires, prepared through a straightforward thermal oxidation process, exhibit a unique yet smooth surface. The structural characterizations confirm the formation of pure-phase TeO2 after the anneal oxidation. TeO2 nanowires are extremely sensitive to NO2 gas, and the maximum response (defined as the ratio of resistance in the air to that under the target gas) to NO2 (10 ppm) is 1.559. In addition, TeO2 nanowire-based sensors can return to the initial state in about 6–7 s at 100 °C. The high sensitivity can be attributed to the length–diameter rate, which adsorbs more NO2 to facilitate the electron transfer. The fast recovery is due to the smooth surface without pores on TeO2 nanowires, which may release NO2 quickly after stopping the gas supply. The present approach for sensing TeO2 nanowires can be extended to other sensor systems as an efficient, accurate, and low-priced tactic to enhance sensor performance.
Impact of Work and Non-Work Support on Employee Well-Being: The Moderating Role of Perceived Organizational Support
Authors: Le Huong , Gopalan Neena , Lee Joohan , Kirige Isuru , Haque Amlan , Yadav Vanita , Lambropoulos Victoria
Published: November 2023

Familial and organizational support are important for employees to sustain quality of life and workplace performance. Grounded in the conservation of resources theory, this study explored the underlying mechanism between family member support and employee well-being. Specifically, we examined how the relationship is mediated by psychological capital, and how perceived organizational support amplifies the effect of familial support on psychological capital. Using survey data from 231 Vietnamese employees, results showed that family member support positively predicted employee well-being. This relationship was mediated by psychological capital. Additionally, the relationship between family member support and psychological capital was moderated by perceived organizational support, becoming stronger under conditions of high organizational support. This study offers important theoretical implications regarding the roles of family and organizational support in impacting employee well-being and happiness. Additionally, it provides practical implications for strategic human resource management, highlighting approaches to develop a socially sustainable work environment that promotes employee well-being.
Optimizing an Urban Green Space Ecological Network by Coupling Structural and Functional Connectivity: A Case for Biodiversity Conservation Planning
Authors: Zhou Yuan , Yao Jing , Chen Mingkun , Tang Mi
Published: November 2023

Strengthening and optimizing the spatial structure and functional connectivity of green space ecological networks can not only relieve the tight urban space and provide biodiversity protection but also promote the virtuous cycle of the urban ecosystem and provide a new method for the resilient development of the urban landscape. In this study, the central area of Chengdu was taken as the study area; Morphological Spatial Pattern Analysis (MSPA) with landscape metrics were combined to determine the optimal distance threshold and identify the ecological sources. Graph theory and circuit theory were applied to construct and optimize the green space ecological network with structural or functional connectivity, respectively. Based on the coupling effect, the optimization of the ecological network was put forward, and the network analysis method was used to evaluate the connectivity of three different types of ecological networks. The results were as follows: (1) The ecological network with structural connectivity was composed of 74 stepping stones, 43 protective sources, and 315 ecological corridors. The connectivity of green space structures gradually decreased from west to east and from periphery to center. (2) In the optimal ecological network with functional connectivity, 176 important ecological corridors were protected, and 40 pinch points and 48 protective sources were identified. The number of important corridors in the east and south was the largest, and the network structure was relatively complex. The barriers were divided into three different levels of ecological restoration areas. (3) The green ecological network with structural and functional connectivity has the best network connectivity. A green space ring network optimization pattern of one center, two belts, multi-points, multi-corridors, and multi-zones connected in a series was proposed. It was suggested to build a multi-level forest ecosystem in Longquan Mountain, develop eco-fruit agriculture and eco-tourism, enrich the biodiversity of the ecological source, and improve its anti-interference ability to the external environment. It is also important to increase ecological strategic points and stepping stones to strengthen the links between different ecological restoration areas, properly handle the use of cultivated land in different regions, strictly observe the red line of cultivated land, and maintain the integrity and diversity of ecological sources. Therefore, the optimization method of the green space ecological network in this study provides technical support for the effective determination of ecological protection areas, the accurate implementation of green space ecological networks, and a scientific planning strategy for decision-makers.
Systematic Review of Contaminants of Emerging Concern (CECs): Distribution, Risks, and Implications for Water Quality and Health
Authors: Feng Weiying , Deng Yuxin , Yang Fang , Miao Qingfeng , Ngien Su Kong
Published: November 2023

The introduction of contaminants of emerging concern (CECs) into the environment has raised concerns due to the significant risks they pose to both ecosystems and human health. In this sys-tematic review, we investigate research trends on CECs worldwide over the past 10 years, focus-ing on four critical aspects: (i) the identification and distribution of typical CECs across various media, (ii) the sources and environmental behavior of CECs, (iii) the implications of CECs expo-sure on human health, and (iv) risk assessment and control measures for CECs. The review re-veals a comprehensive understanding of the typical types and distribution of CECs in different environmental media, shedding light on their prevalence and potential impact on ecosystems. Furthermore, insights into the sources and behavior of CECs provide crucial information for de-vising effective strategies to mitigate their release into the environment. By examining the health effects of EC exposure, we highlight the importance of considering potential risks to human well-being. This aspect of the review emphasizes the significance of monitoring and managing CECs to safeguard public health. The review also synthesizes the advancements in risk assessment methodologies and control measures for CECs, which are essential for developing comprehensive regulations and guidelines to manage these contaminants effectively. Drawing from the findings, we identify future research directions for CECs in aquatic environments.
Classification of Water Reservoirs in Terms of Ice Phenomena Using Advanced Statistical Methods—The Case of the Silesian Upland (Southern Poland)
Authors: Rzetala Mariusz , Topolski Mariusz , Solarski Maksymilian
Published: November 2023

Ice phenomena occurring in water bodies are an important indicator of natural changes (e.g., climate change) and the possibilities for economic use of water bodies (e.g., using the ice cover); hence, there is a need to adopt new advanced statistical methods for the purpose of their analysis and assessment. Material for this study was collected for three winter seasons in 39 water bodies in the Silesian Upland (southern Poland). Nine variables were used in the analysis, of which three pertained to the features of the water bodies studied (surface area, mean depth, the amount of water retained), and six pertained patterns to of ice phenomena (average near-surface water temperature during ice phenomena, average and maximum ice thickness, the number of days with ice phenomena, the number of days with ice cover, and average thickness of the snow accumulated on ice). The centroid class principal component analysis (CCPCA) method was found to be the most precise of the five methods used in the study for classifying water bodies in terms of their ice regimes. It enabled the most accurate division of the group of water bodies covered by the study in terms of their ice regimes in conjunction with their morphometric features and hydrological types. The presented method of classifying water bodies using advanced statistical methods is an original proposal, which was used for the first time in limnological research and in the analysis of ice phenomena.