bluuebunny/crossref_metadata_2025 huggingface dataset:
The Value of Ultrasound Diagnostic Imaging of Anterior Crucial Ligament Tears Verified Using Experimental and Arthroscopic Investigations
Authors: Wasilczyk Cezary
Published: January 2024

This study investigates the potential of the ultrasound imaging technique in the assessment of Anterior Cruciate Ligament (ACL) pathologies by standardizing the examination process. We focused on four key ultrasound parameters: the inclination of the ACL; swelling or scarring at the ACL’s proximal attachment to the lateral femoral condyle; swelling or scarring of the ACL/posterior cruciate ligament (PCL) compartment complex with accompanying morphological changes in the posterior joint capsule; and dynamic instability, categorized into three ranges—0–2 mm, 3–4 mm, and ≥5 mm. The study group consisted of 25 patients with an ACL injury and 25 controls. All four tested parameters were found more frequently in the study group compared to the control (p < 0.0001). Our findings suggest that this standardized approach significantly augments the diagnostic capabilities of ultrasound, complementing clinical evaluation and magnetic resonance imaging (MRI) findings. The meticulous assessment of these parameters proved crucial in identifying subtle ACL pathologies, which might otherwise be missed in conventional imaging modalities. Notably, the quantification of dynamic instability and the evaluation of morphological changes were instrumental in early detection of ACL injuries, thereby facilitating more precise and effective treatment planning. This study underscores the importance of a standardized ultrasound protocol in the accurate diagnosis and management of ACL injuries, proposing a more comprehensive diagnostic tool for clinicians in the field of sports medicine and orthopedics.
Feasibility and Performance Analysis of Cylinder Deactivation for a Heavy-Duty Compressed Natural Gas Engine
Authors: Misul Daniela , Scopelliti Alex , Di Maio Dario , Napolitano Pierpaolo , Beatrice Carlo
Published: January 2024

The rising interest in the use of gaseous fuels, such as bio-methane and hydro-methane, in Heavy-Duty (HD) engines to reduce Greenhouse Gases pushed by the net-zero CO2 emissions roadmap, introduced the need for appropriate strategies in terms of fuel economy and emissions reduction. The present work hence aims at analysing the potential benefits derived from the application of the cylinder deactivation strategy on a six-cylinder HD Natural Gas Spark Ignition (SI) engine, typically employed in buses and trucks. The activity stems from an extensive experimental characterisation of the engine, which allowed for validating a related 1D model at several Steady-State conditions over the entire engine workplan and during dynamic phases, represented by the World Harmonized Transient Cycle (WHTC) homologation cycle. The validated model was exploited to assess the feasibility of the considered strategy, with specific attention to the engine working areas at partial load and monitoring the main performance parameters. Moreover, the introduction in the model of an additional pipeline and of valves actuated by a dedicated control logic, allowed for embedding the capability of using Exhaust Gas Recirculation (EGR). In the identified operating zones, the EGR strategy has shown significant benefits in terms of fuel consumption, with a reduction of up to 10%. Simultaneously, an appreciable increase in the exhaust gas temperature was detected, which may eventually contribute to enhance the Three-Way Catalyst (TWC) conversion efficiency. Considering that few efforts are to be found in the literature but for the application of the cylinder deactivation strategy to Light-Duty or conventionally fuelled vehicles, the present work lays the foundation for a possible application of such technology in Natural Gas Heavy-Duty engines, providing important insights to maximise the efficiency of the entire system.
Comparative Analysis of Subjective Indoor Environment Assessment in Actual and Simulated Conditions
Authors: Orman Łukasz Jan , Siwczuk Natalia , Radek Norbert , Honus Stanislav , Piotrowski Jerzy Zbigniew , Dębska Luiza
Published: January 2024

This paper experimentally analyses an indoor environment assessment of a large group of respondents regarding their subjective perception of overall comfort, indoor air quality and humidity. The questionnaire survey was applied as a testing method together with measurements of the physical parameters conducted with a microclimate meter. Two types of environment were analysed: educational rooms and the climate chamber. The comparative analysis of the sensations experienced within them indicates that they generate quite similar responses; however, some discrepancies have been identified. The overall comfort of the climate chamber was typically assessed as being higher than that of the educational rooms at the same air temperature. The most favourable air temperature in the climate chamber was ca. 20.7 °C, while in the educational rooms it was ca. 22.3 °C. The most preferable conditions in the climate chamber occurred at a thermal sensation vote of −0.4 (“pleasantly slightly cool”), while in the educational rooms it occurred at +0.2 (“neutral/pleasantly slightly warm”). Quite strong correlations between overall comfort and indoor air quality as well as between humidity assessment and humidity preference votes were observed, which did not seem to depend on the type of environment. These findings are important because results from the simulated conditions are often used in the analyses of actual living/working environments.
A Two-Layer Control Strategy for the Participation of Energy Storage Battery Systems in Grid Frequency Regulation
Authors: Zhang Pan , Xin Shijin , Wang Yunwen , Xu Qing , Chen Chunsheng , Chen Wei , Dong Haiying
Published: January 2024

A two-layer control strategy for the participation of multiple battery energy storage systems in the secondary frequency regulation of the grid is proposed to address the frequency fluctuation problem caused by the power dynamic imbalance between the power system and load when a large number of new energy sources are connected to the grid. A comprehensive allocation model based on area regulation requirement (ARR) signals and area control error (ACE) signals is proposed to obtain the total output of the secondary frequency modulation (FM) demand with a higher degree of adaptation when the FM units respond to the automatic generation control command, and the total output is reasonably allocated to each FM unit by using the two-layer control. Considering the dynamic fluctuation of the grid frequency, the fluctuation is dynamically suppressed in real-time by applying model predictive control to successfully forecast the frequency deviation while realizing the deviation-free correction in the frequency dynamic correction layer. The optimal power distribution of FM units based on the distributed control concept, as well as the power depth of each unit, are coordinated in the equalization control layer while keeping a decent battery charge level. Finally, in Matlab/Simulink, the proposed control approach is simulated and validated. The findings show that the suggested control approach can suppress frequency difference fluctuation, keep the battery charged, and reduce the unit’s FM loss.
Photovoltaic Power Generation Forecasting with Hidden Markov Model and Long Short-Term Memory in MISO and SISO Configurations
Authors: Delgado Carlos J. , Alfaro-Mejía Estefanía , Manian Vidya , O’Neill-Carrillo Efrain , Andrade Fabio
Published: January 2024

Photovoltaic (PV) power generation forecasting is an important research topic, aiming to mitigate variability caused by weather conditions and improve power generation planning. Climate factors, including solar irradiance, temperature, and cloud cover, influence the energy conversion achieved by PV systems. Long-term weather forecasting improves PV power generation planning, while short-term forecasting enhances control methods, such as managing ramp rates. The stochastic nature of weather variables poses a challenge for linear regression methods. Consequently, advanced, state-of-the-art machine learning (ML) approaches capable of handling non-linear data, such as long short-term memory (LSTM), have emerged. This paper introduces the implementation of a multivariate machine learning model to forecast PV power generation, considering multiple weather variables. A deep learning solution was implemented to analyze weather variables in a short time horizon. Utilizing a hidden Markov model for data preprocessing, an LSTM model was trained using the Alice Spring dataset provided by DKA Solar Center. The proposed workflow demonstrated superior performance compared to the results obtained by state-of-the-art methods, including support vector machine, radiation classification coordinate with LSTM (RCC-LSTM), and ESNCNN specifically concerning the proposed multi-input single-output LSTM model. This improvement is attributed to incorporating input features such as active power, temperature, humidity, horizontal and diffuse irradiance, and wind direction, with active power serving as the output variable. The proposed workflow achieved a mean square error (MSE) of 2.17×10−7, a root mean square error (RMSE) of 4.65×10−4, and a mean absolute error (MAE) of 4.04×10−4.
Recycling Fermentation Strategy for Waste Cellular Residues in the Production of Polyunsaturated Fatty Acids
Authors: Yin Feng-Wei , Huang Jiao , Zhan Ci-Tong , Sun Xiao-Long , Zheng Wei-Long , Luo Xi , Zhang Ying-Ying , Yin Long-Fei , Fu Yong-Qian
Published: January 2024

Cellular residues after lipid extraction are the primary waste by-products of microbial polyunsaturated fatty acid production. To mitigate the discharge of cellular residues and reduce the cost of DHA and ARA production, this study examined the utilization of enzymatically hydrolyzed Aurantiochytrium cellular residues (ACRs) and Mortierella alpina cellular residues (MCRs) as nitrogen sources. Results demonstrated that ACRs and MCRs could partially substitute yeast extract (YE) without adverse effects on DHA and ARA fermentation. Moreover, the implementation of a new fermentation medium incorporating ACRs, MCRs, and YE as mixed nitrogen sources resulted in DHA and ARA yields of 17.78 and 5.77 g/L, respectively. These values represented increases of 10.37% and 9.28% compared to traditional cultural methods, while simultaneously reducing the usage of YE by 80% and 60%, respectively. Therefore, the strategy of recycling waste cellular residues presents a novel approach for reducing the costs and environmental impact associated with microbial fermentations.
Two-Step Optimization for Improving Prodigiosin Production Using a Fermentation Medium for Serratia marcescens and an Extraction Process
Authors: Wang Xin , Cui Zhihao , Zhang Zongyu , Zhao Jiacheng , Liu Xiaoquan , Meng Guangfan , Zhang Jing , Zhang Jie
Published: January 2024

Prodigiosin (PG) is a secondary metabolite produced by Serratia marcescens which has a promising future in food, textile, and other industries due to its bright color and diverse biological activities. Currently, the production of PG is mainly restricted by the components of the fermentation medium and large losses during its extraction process, making large-scale industrial production impossible. In this study, a Box–Behnken design (BBD) was used to optimize the response surface of the fermentation medium of S. marcescens. The optimum medium composition was found to be sucrose, 16.29 g/L; peptone, 11.76 g/L; and tween 80, 2.64 g/L. This composition produced a PG amount of 1653.95 ± 32.12 mg/L, which is a 64-fold increase compared to the initial medium. A Box–Behnken design (BBD) was then used to optimize the response surface of the extraction process of PG, aiming to reduce loss during extraction. The optimal extraction conditions were determined to be a solvent fermentation liquid ratio of 9.12:1, an extraction temperature of 25.35 °C, and an extraction time of 30.33 min. These conditions resulted in a final PG production amount of 2142.75 ± 12.55 mg/L, which was nearly 84 times higher than the initial production amount of PG. These results provide essential theoretical and experimental support for the industrial production of PG.
First Report of Vibrio vulnificus Outbreak in Farm-Raised Sorubim (Pseudoplatystoma sp.) from Brazil
Authors: Janampa-Sarmiento Peter C. , Reis Francisco Y. T. , Egger Renata C. , de Pádua Santiago B. , Marcelino Sóstenes A. C. , Cunha João L. R. , Pierezan Felipe , Figueiredo Henrique C. P. , Tavares Guilherme C.
Published: January 2024

Vibrio vulnificus is an opportunistic pathogen in humans and exhibits pathogenic behavior in several aquaculture fish species. To date, in Brazil, there are no reports of V. vulnificus outbreaks in farmed fish. However, in 2019, sorubim (Pseudoplatystoma sp.) in the grow-out phase with clinical signs and cumulative mortality of 40% was registered. We aimed to identify and characterize the etiological agents of this outbreak. Seven moribund fish were sampled for bacteriological studies, and isolates were obtained from the brain and kidneys. Bacterial identification using matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF/MS) revealed V. vulnificus. One isolate, RP4, was used for identification via dnaJ and 16S rRNA gene sequencing and antimicrobial susceptibility evaluation. Sorubim juveniles were experimentally challenged with RP4 isolate via intracelomic injection (IC, 107 colony-forming units [CFU] fish−1) and immersion bath (IB, 106 CFU mL−1 for 30 min). Identities to V. vulnificus of dnaJ and 16S rRNA genes by BLAST analysis were higher than 92% and 98%, respectively. Susceptibility to oxytetracycline, florfenicol, and other antimicrobial molecules was also observed. In the IC-challenged group, the mortality rate was 100% with V. vulnificus recovered from fish organs (brain, liver, spleen, and kidney). Additionally, splenic endothelium alterations were observed in the IC group. On the contrary, the control and IB groups did not develop any clinical signs, mortality, or bacterial recovery after 7 days of challenge. To the best of our knowledge, this is the first report of pathogenic V. vulnificus in farmed Pseudoplatystoma sp.
Changes in Milk Protein Functionality at Low Temperatures and Rennet Concentrations
Authors: Hamouda Mahmoud E. A. , Salunke Prafulla
Published: January 2024

This study aimed to evaluate the influence of low-concentration rennet on the chemical, rheological characteristics, and protein fractions of skim milk (SM) at 4 ± 1 °C. Skimmed milk (SM) was divided into four lots of 500 mL, and diluted rennet (1:10,000) was added at different levels at 4 ± 1 °C. The treatments included control (no rennet), T1 (0.001 mL/rennet), T2 (0.01 mL rennet), and T3 (0.1 mL rennet) treatments, which were incubated for 24 h. The sampling was performed at 0, 1, 2, 6, 12, and 24 h, and the SM after incubation time was heated to 73 °C/16 s to denature the rennet enzyme. Skim milk samples (SMS) (control and rennet-added samples) were evaluated for proximate composition, capillary gel electrophoresis (CGE), hydrodynamic diameter, zeta potential, and rheology at 0, 1, 2, 6, 12, and 24 h. Foaming ability, foaming stability, water-holding capacity (WHC), oil emulsifying activity (OEA), and emulsion stability (ES) were performed at 0, 12, and 24 h of incubation time. There was a significant (p < 0.05) increase in non-proteins by 0.50% and in non-casein nitrogen by 0.81% as incubation progressed. The results showed that aggregation or curd was not formed during storage time. The CGE data indicated that increasing the rennet concentration had a significant (p < 0.05) effect on decreasing κ-CN, and breakdown increased at higher levels of rennet usage. There was a significant (p < 0.05) increase in the hydrodynamic diameter and a decrease in the zeta potential values in rennet-added samples at the end of the incubation time (24 h). The rheological results showed no changes in the storage modulus (G′), loss modulus (G″), or viscosity values. Increasing the rennet amount and storage time led to a significant (p < 0.05) decrease in the foaming ability and foaming stability and a significant (p < 0.05) increase in the oil emulsifying activity and emulsion stability of rennet-added SMS. This study concluded that milk protein functionality can be changed without aggregating or curd formation, and rennet milk can be processed.
Impact of COVID-19 on Perinatal Outcomes and Birth Locations in a Large US Metropolitan Area
Authors: Lee Esther G. , Perez Alejandra , Patel Arth , Patel Aloka L. , Waters Thaddeus , Fricchione Marielle , Johnson Tricia J.
Published: January 2024

This was a population-based study to determine the impact of COVID-19 on birth outcomes in the Chicago metropolitan area, comparing pre-pandemic (April–September 2019) versus pandemic (April–September 2020) births. Multivariable regression models that adjusted for demographic and neighborhood characteristics were used to estimate the marginal effects of COVID-19 on intrauterine fetal demise (IUFD)/stillbirth, preterm birth, birth hospital designation, and maternal and infant hospital length of stay (LOS). There were no differences in IUFD/stillbirths or preterm births between eras. Commercially insured preterm and term infants were 4.8 percentage points (2.3, 7.4) and 3.4 percentage points (2.5, 4.2) more likely to be born in an academic medical center during the pandemic, while Medicaid-insured preterm and term infants were 3.6 percentage points less likely (−6.5, −0.7) and 1.8 percentage points less likely (−2.8, −0.9) to be born in an academic medical center compared to the pre-pandemic era. Infant LOS decreased from 2.4 to 2.2 days (−0.35, −0.20), maternal LOS for indicated PTBs decreased from 5.6 to 5.0 days (−0.94, −0.19), and term births decreased from 2.5 to 2.3 days (−0.21, −0.17). The pandemic had a significant effect on the location of births that may have exacerbated health inequities that continue into childhood.
Challenges in Transition of Care for People with Variations in Sex Characteristics in the European Context
Authors: Gramc Martin
Published: January 2024

Objective: People with variations in sex characteristics (VSCs) have been receiving inadequate care for many decades. The Chicago consensus statement in 2006 aimed to introduce improved comprehensive care, which would include the transition of care from pediatric to adult services organized by multidisciplinary teams. Yet, the evidence for transitional care is scarce. The aim of this paper is to outline the delivery of transition of care for adolescents and young adults with VSCs. Method: Seven focus groups were conducted with health care professionals and peer support groups by care teams in Central, Northern, and Western Europe. The data from the focus groups were examined using reflexive thematic analysis. Results: Even though the transition of care has been implemented in the last two decades, it remains inadequate. There are differences among countries, as the quality of care depends on available resources and variations in sex characteristics. Moreover, there are significant hurdles to adequate transition of care, as there is lack of time and funding. The lack of adult care providers and psychosocial support often leaves young adults with VSCs to navigate the health care system alone. Conclusion: The outcome of the study shows that the transition of care is organized through the department of pediatric endocrinology. The quality of care varies due to resources and variations in sex characteristics. A lack of adult specialists, and especially psychosocial support, represents the biggest obstacle for young adults and adults in navigating the health care system and for improvements in the provision of health care to adults. There is a risk of re-traumatization, as adolescents and young adults must often repeat their medical history and educate adult care providers who are insufficiently trained and knowledgeable.
Barriers and Mythical Practices of Teenagers Regarding the Prevention of Sexually Transmitted Infections in Rural Areas of Limpopo Province, South Africa
Authors: Damian Jessica Uchechi , Hlungwane Eustacia , Tshitangano Takalani Grace
Published: January 2024

Sexually transmitted infections in South Africa are increasing at alarming rates. This study’s setting is no. 5, with the highest STI, pregnancy, and HIV statistics in Limpopo province among 13- to 19-year-old teenagers. This study explored preventative measures against STIs practiced by teenagers in rural areas of Limpopo province, South Africa. This study was conducted at a selected rural-based clinic using an exploratory descriptive qualitative research approach. Unstructured in-depth face-to-face interviews were used to collect data from sixteen conveniently sampled teenagers aged 13–19, consisting of 13 females and 3 males, who came to Manavhela Clinic for youth-friendly services in August/September 2022. Open-coding analysis was used to identify themes and sub-themes. Measures to ensure trustworthiness were ensured. Ethical clearance (FSH/21/PH/22/2211) was obtained, and ethics principles were observed throughout this study. Two themes emerged from data analysis: STI preventive measures practiced by teenagers and factors influencing the choice of STI preventive measures practiced by teenagers. Only a few participants aged 13 and 14 years of age practiced abstinence and condom use. Most participants were sexually active and used mythical mixtures made from boiling aloe or morula tree (which they drank before and after sex), applied plain yogurt on the vagina once a week, or practiced vaginal steaming. Participants cited patriarchy, lack of sex education in rural schools, long distances to clinics, and desire to taste sex as reasons for adopting the practiced preventive measures. Risky sexual behavior among 13- to 19-year-old teenagers is still rife in rural areas. Rural clinics in Limpopo province should intensify STI school health education and youth-friendly services programs to raise awareness and improve accessibility to condoms.
A Study on the Development of Two Ornamental Varieties of Ipomoea batatas Cultivated in Vertical Systems in the Northeastern Region of Europe
Authors: Cojocariu Mirela , Marta Alina Elena , Jităreanu Carmenica Doina , Chelariu Elena-Liliana , Căpşună Sorin , Cara Irina Gabriela , Amișculesei Petronica , Istrate Ana-Maria-Roxana , Chiruță Ciprian
Published: January 2024

The Ipomoea batatas ornamental variety of the edible sweet potato is a decorative sweet potato with exquisite leaves, available in different varieties. Due to its numerous characteristics related to resistance, adaptability to environmental conditions, rapid growth, and aesthetic traits, it has attracted the interest of researchers. The purpose of this study is to investigate the growth and development behaviour of two varieties of I. batatas, namely ‘Margarita’ and ‘Blackie’, cultivated in vertical systems, to provide guidelines for their usage in green façade design. The physiological growth processes throughout the growing season were analysed by monitoring three biometric parameters (diameter, height, and leaf count), transpiration (dehydration rate), and the photosynthesis process (photosynthetic pigment content) for treated and control variants and four cardinal orientations (N, E, S, and W). Compared to the control and western orientation, which proved to be less advantageous, the northern orientation and treatment appeared to be the most suitable options for both varieties analysed. Also, the content of organic substances was more intense in the Cropmax-treated ‘Blackie’, especially on the northern façade, with the highest quantities of chlorophyll a (17.1%), chlorophyll b (9.1%), and carotenoid pigments (7.5%). In addition, the leaf dehydration rate, as well as the amount of free water loss, were reduced on the northern façade of ‘Blackie’. Therefore, ‘Blackie’ can be recommended as a variety distinguished by its improved resistance to challenging environmental conditions, particularly drought.
Barriers to Women’s Menstrual Hygiene Practices during Recurrent Disasters and Displacement: A Qualitative Study
Authors: Hirani Shela Akbar Ali
Published: January 2024

Disasters that involve displacement are particularly challenging for managing personal and menstrual hygiene, which can increase the risk of toxic shock syndrome, infections and other health conditions that can endanger women’s lives. This study aimed to examine the menstrual hygiene challenges experienced by internally displaced women affected by recurrent natural disasters and subsequent displacement in the context of a low–middle-income country, i.e., Pakistan. A critical ethnographic study was undertaken in disaster-relief camps in the northern region of Pakistan. Data were collected using multiple methods, including field observations, review of media reports and in-depth interviews with 18 women. The findings suggested that the key barriers to the personal and menstrual hygiene of women during recurrent disasters and displacement in the mountainous rural region of Pakistan include inadequate housing, lack of infrastructure and humanitarian aid, no waste disposal system and lack of women-friendly spaces in disaster-relief camps. Community-based collaboration is necessary for the implementation of effective interventions. A comprehensive menstrual response to promote the health and well-being of women during disasters must include menstruation supplies, supportive facilities (mainly toilets and bathing facilities), supplementary supplies for storing, washing and drying, disposal/waste management facilities, education and culturally appropriate spaces and supplies.
Comparative Study on the Periodontal Parameters Used in Diagnosing Periodontitis in Puerperae and Periodontitis’ Relationship with the Birth of Preterm Infants: A Case-Control Study
Authors: de Vasconcelos Calixto Nayra Rodrigues , Lopes Fernanda Ferreira , Franco Marcela Mayana Pereira , Gomes-Filho Isaac Suzart , Benatti Bruno Braga , Alves Cláudia Maria Coêlho
Published: January 2024

To compare different criteria for the diagnosis of periodontitis and to evaluate the association of this condition with prematurity, this case-control study was conducted on 283 mothers of infants, divided into two groups based on gestational age (cases: <37 weeks, controls: ≥37 weeks), with 71 cases and 212 controls. The periodontal evaluation included probing depth (PD), clinical attachment level (CAL), plaque index, and bleeding on probing (BOP). Participants were classified regarding periodontitis per 14 criteria based on different periodontal parameters. The criterion selected as the gold standard was the presence of at least four teeth with one or more sites with a PD ≥ 4 mm, CAL ≥ 3 mm, and BOP at the same site. The prevalence of periodontal disease ranged from 8.1% to 55.1%. Moreover, compared to the gold standard, the sensitivities of the other criteria were 100%, while specificity ranged from 50.4% to 96.4%. Periodontitis, defined by six of the selected criteria, was associated with prematurity after multivariate adjustment, with OR ranging from 1.85 to 2.69 and 95% CI from 1.01 to 5.56; one of them was the gold standard mentioned above. Measurements using the clinical parameters of PD, CAL, and bleeding at the same site (criteria 5, 6, 7, 8), CPI (criterion 10), and at least four teeth with a PD ≥ 4 mm and CAL ≥ 3 mm (criterion 11) to define periodontitis showed a statistically significant association (p < 0.05). Given this study’s limitations, we can conclude that the diagnostic criteria for a periodontitis definition using a PD ≥ 4 mm and CAL ≥ 3 mm in two or more teeth, with BOP at the same site, seem stronger when detecting an association between periodontitis and prematurity.
Retinoic Acid Action in Cumulus Cells: Implications for Oocyte Development and In Vitro Fertilization
Authors: Sidell Neil , Rajakumar Augustine
Published: January 2024

In the field of human in vitro fertilization (IVF), selecting the best oocyte for freezing or embryo for transfer remains an important focus of clinical practice. Although several techniques are and have been used for this goal, results have generally not been favorable and/or are invasive such that damage to some embryos occurs, resulting in a reduced number of healthy births. Therefore, the search continues for non-invasive oocyte and embryo quality markers that signal the development of high-quality embryos. Multiple studies indicate the important positive effects of retinoic acid (RA) on oocyte maturation and function. We previously showed that a high follicular fluid (FF) RA concentration at the time of oocyte retrieval in IVF protocols was associated with oocytes, giving rise to the highest quality embryos, and that cumulus granulosa cells (CGCs) are the primary source of follicle RA synthesis. Data also demonstrated that connexin-43 (Cx43), the main connexin that forms gap junctions in CGCs, is regulated by RA and that RA induces a rapid increase in gap junction communication. Here, we hypothesize that CGC RA plays a causal role in oocyte competency through its action on Cx43 and, as such, may serve as a biomarker of oocyte competence. Multiple studies have demonstrated the requirement for Cx43 in CGCs for the normal progression of folliculogenesis, and that the increased expression of this connexin is linked to the improved developmental competence of the oocyte. The data have shown that RA can up-regulate gap junction intercellular communication (GJIC) in the cumulus–oocyte complex via a non-genomic mechanism that results in the dephosphorylation of Cx43 and enhanced GJIC. Recognizing the positive role played by gap junctions in CGCs in oocyte development and the regulation of Cx43 by RA, the findings have highlighted the possibility that CGC RA levels may serve as a non-invasive indicator for selecting high-quality oocytes for IVF procedures. In addition, the data suggest that the manipulation of Cx43 with retinoid compounds could provide new pharmacological approaches to improve IVF outcomes in cases of failed implantation, recurrent miscarriage, or in certain diseases that are characterized by reduced fecundity, such as endometriosis.
Leading Risk Factors for Congenital Deafness in the Context of Universal Neonatal Screening: Our Observations in a Four-Year Retrospective Study
Authors: Paul Antoine , Bense Fanny , Boithias Guerot Claire , De La Rubia Sofia , Lebeaux Cécile , Papon Jean-François
Published: January 2024

It has been demonstrated that universal hearing neonatal screening (UHNS) is efficient at providing early diagnosis and rehabilitation for deafness. The risk factors of deafness in children have been identified, but less specifically in those diagnosed after UHNS. In this study, we aim to study these factors in infants who were referred after screening and to compare our experience to recent data. We studied infants referred to our department for diagnosis after screening between January 2018 and December 2021. Their medical history and neonatal hearing risk factors were assessed. Associations between factors were also analyzed. Sixty-six infants were included. A family history of deafness (47%), syndromic deafness (41%), intrauterine growth retardation or prematurity (19.7%), and prolonged NICU admission (18%) were the most observed factors. When analyzing according to these associations, family history of deafness and syndromic cases remained the most prevalent factors (74%), while only five cases (7.8%) presented with other neonatal risk factors only. The majority of congenital hearing loss cases are observed in infants with suspected genetic deafness. Parental counseling, the diagnostic pathway, as well as the healthcare system should be adapted according to these risk factors.
Effects of Relative Humidity and Temperature on the Drying Shrinkage of Roller-Compacted Concrete Pavements
Authors: Pulecio-Díaz Julián , Sol-Sánchez Miguel , Moreno-Navarro Fernando
Published: January 2024

Roller-compacted concrete (RCC) pavements have been the subject of studies focused on their increasing deterioration over time due to the influence of vehicular loading and ambient factors in humidity and temperature conditions ranging from medium to low (40% relative humidity and 25 °C temperature). Therefore, it is necessary to understand how they behave under various relative humidity and temperature conditions since these parameters vary in each geographic region. In this context, this research focused on analyzing the effect of drying shrinkage on RCC pavements under the influence of vehicular loading using a computational model calibrated with data obtained under typical ambient conditions. For this purpose, laboratory experiments were performed, numerical modeling was used, and the results for RCC pavements were validated using statistical analysis. The results revealed validated models providing moisture content and drying shrinkage curves. These results also underline the importance of considering ambient effects when calculating pavement stresses as a response variable in structural designs. In particular, these effects are highlighted as they can generate changes in pavement stresses of up to 10%, emphasizing the relevance of the models proposed in this study as they consider this phenomenon when predicting the performance and durability of RCC pavements.
The Trochlear Paradox in Native Knees and Its Potential Impact on Total Knee Arthroplasty: An MRI-Based Correlation Study Investigating the Effect of Varying Posterior Femoral Condyle Angles on the Patellofemoral Joint
Authors: Röttinger Timon , Lisitano Leonard , Abelmann-Brockmann Johanna , Rau Kim , Koenemann Nora , Fenwick Annabel , Mayr Edgar , Röttinger Heinz
Published: January 2024

Background: Since the beginning of total knee arthroplasty, implant alignment has been a central point of discussion. As diverse as the discussed alignment theories are, as uniform is the implant design, which is supposed to be based on the “average knee.” Steady upgrades in prosthesis design and modern alignment theories have achieved improvements. However, knee arthroplasty continues to be burdened by a significant percentage of dissatisfied patients. In current knee arthroplasty, femoral implant alignment is referenced by the dorsal and distal condylar axes. The patellofemoral joint variance is not sufficiently considered. Predominantly dorsal and distal referencing at the femoral condyle determines the postoperative shape of the anterior knee joint. The present study investigated a possible relationship between dorsal and distal joint parameters and the patellofemoral joint. Methods: In this explorative retrospective monocentric study, MRI cross-sectional images of 100 native knee joints were evaluated. By determining parametric correlations according to Pearson, the study investigates whether the independent variables “posterior femoral condyle angle” and “lateral distal femoral angle” are related to “lateral trochlear inclination”, “patella tilt”, and “bisect offset”. Results: The posterior condylar angle significantly correlates with lateral trochlear inclination, patella tilt, and bisect offset. There is a positive correlation with patella tilt and bisect offset but a negative correlation with lateral trochlear inclination. The lateral distal femoral angle did not correlate with the studied parameters. Conclusion: The lateral trochlear inclination decreases with an increased posterior femoral condylar angle. The posterior referencing of the femoral component in total knee arthroplasty simultaneously establishes the shape of the anterior knee joint. Our results indicate that increasing posterior condyle angles significantly correlate with flattened lateral trochlear inclinations in native knees and suggest a systematic biomechanical conflict in total knee arthroplasty.
Enhancing Perceptual—Motor Skills in Sports: The Role of Ecological Sounds
Authors: Agostini Tiziano , Sors Fabrizio , Murgia Mauro , Galmonte Alessandra
Published: January 2024

Starting approximately from the beginning of the new millennium, a series of studies highlighted that auditory information deriving from biological motion can significantly influence the behavioral, cognitive and neurophysiological processes involved in the perception and execution of complex movements. In particular, it was observed that an appropriate use of sounds deriving from one’s own movement promotes improvements in the movement execution itself. Two main approaches can be used, namely the sonification one or the ecological sound one; the former is based on the conversion of physiological and/or physical movement data into sound, while the latter is based on the use of auditory recordings of movement sounds as models. In the present article, some of the main applications of both approaches—especially the latter—to the domains of sport and motor rehabilitation are reviewed, with the aim of addressing two questions: Is it possible to consider rhythm as a Gestalt of human movement? If so, is it possible to build up cognitive strategies to improve/standardize movement performance from this Gestalt? As with most topics in science, a definitive answer is not possible, yet the evidence leads us to lean toward a positive answer to both questions.
A Comparative Study on the Cam Relationship for the Optimal Vibration and Efficiency of a Kaplan Turbine
Authors: Deng Sen , Zhao Weiqiang , Huang Tianbao , Xia Ming , Wang Zhengwei
Published: January 2024

Kaplan turbines are generally used in working conditions with a high flow and low head. These are a type of axial-flow hydro turbine that can adjust the opening of the guide vanes and blades simultaneously in order to achieve higher efficiency under a wider range of loads. Different combinations of the opening of the guide vanes and blades (cam relationship) will lead to changes in the efficiency of the turbine unit as well as its vibration characteristics. A bad cam relationship will cause the low efficiency or unstable operation of the turbine. In this study, the relative efficiency and vibration of a large-scale Kaplan turbine with 200 MW output were tested with different guide vane and blade openings. The selection of the cam relationship curve for both optimal efficiency and optimal vibration is discussed. Compared with the cam relationship given by the model test, the prototype cam relationship improves the efficiency and reduces the vibration level. Compared to the optimal efficiency cam relationship, the optimal vibration cam relationship reduces the efficiency of the machine by 1% to 2%, while with the optimal efficiency cam relationship, the vibration of the unit increases significantly. This research provides guidance for the optimization of the regulation of a large adjustable-blade Kaplan turbine unit and improves the overall economic benefits and safety performance of the Kaplan turbine power station.
A Protein Phosphatase 2A-Based Assay to Detect Okadaic Acids and Microcystins
Authors: Ikehara Tsuyoshi , Oshiro Naomasa
Published: January 2024

Okadaic acids (OAs) are causative agents of diarrhetic shellfish poisoning, produced by the dinoflagellates Dinophysis spp. and Prorocentrum spp. Microcystins (MCs) are cyclic heptapeptide hepatotoxins produced by some cyanobacteria genera, including Microcystis spp. Traditionally, toxicity detection and quantification of these natural toxins were performed using a mouse bioassay (MBA); however, this is no longer widely employed owing to its lack of accuracy, sensitivity, and with regard to animal welfare. Therefore, alternative toxicity analyses have been developed based on MCs’ and OAs’ specific inhibition of protein phosphatase 2A (PP2A), using p-nitrophenylphosphate (p-NPP) as a substrate. The assay is simple, inexpensive, ready for use on site, and can be applied to several samples at once. For OA detection, this assay method is appropriate for widespread application as a substitute for MBA, as evidenced by its alignment with the oral toxicity of MBA. In this review, we summarize the structure and function of PP2A, the inhibitory activities of OAs and MCs against PP2A, and the practical applications of the PP2A assay, with the aim of improving understanding of the PP2A assay as an OAs and MCs detection and quantification method, as well as its suitability for screening before confirmatory chemical analysis.
A Quick reCAP: Discovering Cryptococcus neoformans Capsule Mutants
Authors: Boodwa-Ko Daphne , Doering Tamara L.
Published: January 2024

Cryptococcus neoformans is an opportunistic fungal pathogen that can cause severe meningoencephalitis in immunocompromised hosts and is a leading cause of death in HIV/AIDS patients. This pathogenic yeast is surrounded by a polysaccharide capsule that is critical for virulence and plays important roles in host-pathogen interactions. Understanding capsule biosynthesis is therefore key to defining the biology of C. neoformans and potentially discovering novel therapeutic targets. By exploiting methods to identify mutants deficient in capsule, June Kwon-Chung and other investigators have discovered numerous genes involved in capsule biosynthesis and regulation. Successful approaches have incorporated combinations of techniques including mutagenesis and systematic gene deletion; complementation and genetic screens; morphological examination, physical separation, and antibody binding; and computational modeling based on gene expression analysis. In this review, we discuss these methods and how they have been used to identify capsule mutants.
The Processing of Multiword Units by Learners of English: Evidence from Pause Placement in Writing Process Data
Authors: Gilquin Gaëtanelle
Published: January 2024

Different methods and sources of information have been proposed in the literature to study the processing of language and, in particular, instances of formulaic language such as multiword units. This article explores the possibility of using pause placement in writing process data to determine the likelihood that a multiword unit is processed as a whole in the mind. The data are texts produced by learners of English and corresponding keylog files from the Process Corpus of English in Education (PROCEED). N-grams are selected on the basis of the finished texts and retrieved from the keylogging data. The pause placement patterns of these n-grams are coded and serve as a basis to compute the Pause Placement and Processing (PPP) score. This score relies on the assumption that n-grams which are delineated but not interrupted by pauses (hence taking the form of ‘bursts of writing’) are more likely to be processed holistically. The PPP score points to structurally complete n-grams such as in fact and first of all as being more likely to be processed holistically than structurally incomplete n-grams such as that we and to the. While the results are plausible and can be further substantiated by characteristics of specific n-grams, it is acknowledged that additional effects might also be at work to explain the results obtained.
Effect of Sulfate Attack on the Expansion Behavior of Cement-Treated Aggregates
Authors: Wang Qi , Liu Jiankun , Wang Pengcheng , Liu Jiangxin , Sun Mingzhi
Published: January 2024

The expansion induced by sulfate attack on cement-treated aggregates (SACA) is a well-known problem that can be solved. It causes obvious heaves in road bases and railway subgrades. In this paper, the effects of the sodium sulfate content, cement content, degree of compaction, sulfate types, attack types, aluminum ion supply, concentration of curing sulfate solution, and temperature on the expansion behavior induced by SACA were investigated over 60 days in the laboratory. Based on the Sobol sensitivity analysis method, the influence of the sensitivity of each factor on the expansion was quantitatively analyzed, and the dominant factor of expansion was proposed. Results show that sulfate content is the domain factor of expansion that is induced by SACA, and it presents a logarithmic function relationship with strain. The 0.5% sodium sulfate content is the minimum sulfate content which causes the expansion that is induced by SACA. When the sulfate content is less than 1%, the expansion induced by SACA is minor. When the sulfate content is between 1% and 3%, the expansion behavior is expressed in four stages as follows: rapid strain increase, followed by a short stagnation period, then a significant strain increase and, finally, constant strain. When the sulfate content is greater than 5%, there are two stages comprising the expansion behavior as follows: the rapid strain increases and constant strain occurs. Greater sulfate content, greater degree of compaction, and lower temperature have positive effects on the expansion induced by SACA. The cement content does not have a consistent effect on expansion behavior. Compared with a sodium sulfate attack, both the reaction rate and expansion of cement-treated aggregates that are attacked by gypsum are smaller, and the attack period is also longer. When the sulfate content is greater than 1%, the addition of kaolin promotes the progression of the expansion induced by SACA. A small amount of water is sufficient for the demand for the sulfate attack. When the sulfate content is at a certain level, the expansion induced by SACA that is under external attack is much smaller than the expansion that is under internal attack. This study is expected to serve as a reference for future research on the mechanics of SACA, and it attempts to provide theoretical support for amending expansions that are induced by SACA.
Flexible Dry Electrode Based on a Wrinkled Surface That Uses Carbon Nanotube/Polymer Composites for Recording Electroencephalograms
Authors: Oh Jihyeon , Nam Kun-Woo , Kim Won-Jin , Kang Byung-Ho , Park Sung-Hoon
Published: January 2024

Electroencephalography (EEG) captures minute electrical signals emanating from the brain. These signals are vulnerable to interference from external noise and dynamic artifacts; hence, accurately recording such signals is challenging. Although dry electrodes are convenient, their signals are of limited quality; consequently, wet electrodes are predominantly used in EEG. Therefore, developing dry electrodes for accurately and stably recording EEG signals is crucial. In this study, we developed flexible dry electrodes using polydimethylsiloxane (PDMS)/carbon-nanotube (CNT) composites with isotropically wrinkled surfaces that effectively combine the advantages of wet and dry electrodes. Adjusting the PDMS crosslinker ratio led to good adhesion, resulting in a highly adhesive CNT/PDMS composite with a low Young’s modulus that exhibited excellent electrical and mechanical properties owing to its ability to conformally contact skin. The isotropically wrinkled surface also effectively controls dynamic artifacts during EEG signal detection and ensures accurate signal analysis. The results of this study demonstrate that dry electrodes based on flexible CNT/PDMS composites and corrugated structures can outperform wet electrodes. The introduction of such electrodes is expected to enable the accurate analysis and monitoring of EEG signals in various scenarios, including clinical trials.