bluuebunny/crossref_metadata_2025 huggingface dataset:
Model of Optimization of Wind Energy Production in the Light of Legal Changes in Poland
Authors: Rabe Marcin , Streimikiene Dalia , Bilan Yuriy
Published: April 2020

The article discusses the planning of wind energy development in the West Pomeranian Region after the introduction of the Act "On investments in wind farms" as of 20 May 2016. The purpose of the article is to provide the forecast of the region’s wind energy development in 2019–2030 by taking into account current legal regulations in Poland. The article proposes an original, optimizing multi-criteria wind energy development model for the studied region, exploring various types of technologies that may appear in the system, taking into account recent legal and political changes in the field of renewable energy regulation in Poland. The results of the optimization model show that the currently passed Act "On investments in wind farms" in Poland actually stopped the development of wind energy in the region. On the other hand, in accordance with the objectives of the adopted draft of the Polish energy policy until 2040, it is expected that the share of renewable energy in electricity production will increase in 2030 in the studied region. Therefore, the paper argues that policy changes are necessary to meet the renewable energy goals of Poland.
In Situ High Pressure Structural Investigation of Sm-Doped Ceria
Authors: Artini Cristina , Massardo Sara , Carnasciali Maria Maddalena , Joseph Boby , Pani Marcella
Published: April 2020

As a result of the lattice mismatch between the oxide itself and the substrate, the high-pressure structural properties of trivalent rare earth (RE)-doped ceria systems help to mimic the compressive/tensile strain in oxide thin films. The high-pressure structural features of Sm-doped ceria were studied by X-ray diffraction experiments performed on Ce1−xSmxO2−x/2 (x = 0.2, 0.3, 0.4, 0.5, 0.6) up to 7 GPa, and the cell volumes were fitted by the third order Vinet equation of state (EoS) at the different pressures obtained from Rietveld refinements. A linear decrease of the ln B 0 vs. ln ( 2 V a t ) trend occurred as expected, but the regression line was much steeper than predicted for oxides, most probably due to the effect of oxygen vacancies arising from charge compensation, which limits the increase of the mean atomic volume ( V a t ) vs. the Sm content. The presence of RE2O3-based cubic microdomains within the sample stiffens the whole structure, making it less compressible with increases in applied pressure. Results are discussed in comparison with ones previously obtained from Lu-doped ceria.
Performance Study on Methanol Steam Reforming Rib Micro-Reactor with Waste Heat Recovery
Authors: Wang Guoqiang , Wang Feng , Chen Bohong
Published: April 2020

Automobile exhaust heat recovery is considered to be an effective means to enhance fuel utilization. The catalytic production of hydrogen by methanol steam reforming is an attractive option for onboard mobile applications, due to its many advantages. However, the reformers of conventional packed bed type suffer from axial temperature gradients and cold spots resulting from severe limitations of mass and heat transfer. These disadvantages limit reformers to a low efficiency of catalyst utilization. A novel rib microreactor was designed for the hydrogen production from methanol steam reforming heated by automobile exhaust, and the effect of inlet exhaust and methanol steam on reactor performance was numerically analyzed in detail, with computational fluid dynamics. The results showed that the best operating parameters were the counter flow, water-to-alcohol (W/A) of 1.3, exhaust inlet velocity of 1.1 m/s, and exhaust inlet temperature of 773 K, when the inlet velocity and inlet temperature of the reactant were 0.1 m/s and 493 K, respectively. At this condition, a methanol conversion of 99.4% and thermal efficiency of 28% were achieved, together with a hydrogen content of 69.6%.
Applications of Triple Active Bridge Converter for Future Grid and Integrated Energy Systems
Authors: Pham Van-Long , Wada Keiji
Published: April 2020

Renewable energy systems and electric vehicles (EVs) are receiving much attention in industrial and scholarly communities owing to their roles in reducing pollutant emissions. Integrated energy systems (IES), which connect different types of renewable energies and storages, have become common in many applications, such as the grid-connected photovoltaic (PV) and battery systems, fuel cells and battery/supercapacitor in EVs. The advantages of all energy sources are maximized by utilizing connection and control strategies. Because many storage systems and household loads are mainly direct current (DC) types, the DC grid has considerable potential for increasing the efficiency of distribution grids in the future. In IES and future DC grid systems, the triple active bridge (TAB) converter is an isolated bidirectional DC-DC converter that has many advantages as a core circuit. Therefore, this paper reviews the characteristics of the TAB converter in current applications and suggests next-generation applications. First, the characteristics and operation modes of the TAB converter are introduced. An overview of all current applications of the TAB converter is then presented. The advantages and challenges of the TAB converter in each application are discussed. Thereafter, the potential future applications of the TAB converter with an adaptable power transmission design are presented.
Techno-Economic Optimization of an Off-Grid Solar/Wind/Battery Hybrid System with a Novel Multi-Objective Differential Evolution Algorithm
Authors: Yang Yong , Li Rong
Published: April 2020

Techno-economic optimization of a standalone solar/wind/battery hybrid system located in Xining, China, is the focus of this paper, and reliable and economic indicators are simultaneously employed to address the problem. To obtain a more precise Pareto set, a novel multi-objective differential evolution algorithm is proposed, where differential evolution with a parameter-adaptive mechanism is applied in the decomposition framework. The algorithm effectiveness is verified by performance comparisons on the benchmark test problems with two reference algorithms: a non-dominated sorting genetic algorithm and a multi-objective evolution algorithm based on decomposition. The applicability of the proposed algorithm for the capacity-optimization problem is also validated by comparisons with the same reference algorithms above, where the true Pareto set of the problem is approximated by combining of the three algorithms through the non-dominant relationship. The results show the proposed algorithm has the lowest inverted generational distance indicator and provides 85% of the true Pareto set. Analyses of the Pareto frontier show that it can produce significant economic benefits by reducing reliability requirements appropriately when loss of power supply probability is less than 0.5%. Furthermore, sensitivity analyses of the initial capital of wind turbine, photovoltaic panel and battery system are performed, and the results show that photovoltaic panel’s initial capital has the greatest impact on levelized cost of electricity, while the initial capital of wind turbine has the least impact.
On the Use of the Coaxial Cylinders Equivalence for the Measurement of Viscosity in Complex Non-Viscometric, Rotational Geometries
Authors: Parlato Regina Miriam , Russo Eliana R. , Läuger Jörg , Costanzo Salvatore , Vanzanella Veronica , Grizzuti Nino
Published: April 2020

The rheology of macroscopic particle suspensions is relevant in many industrial applications, such as cement-based suspensions, synthetic and natural drilling fluids. Rheological measurements for these complex, heterogeneous systems are complicated by a double effect of particle size. On the one hand, the smallest characteristic length of the measuring geometry must be larger than the particle size. On the other hand, large particles are prone to sediment, thus calling for the use of rotational tools that are able to keep the suspension as homogeneous as possible. As a consequence, standard viscometric rotational rheometry cannot be used and complex flow geometries are to be implemented. In this way, however, the flow becomes non-viscometric, thus requiring the development of approximate methods to translate the torque vs. rotation speed raw data, which constitute the rheometer output, into viscosity vs. shear rate curves. In this work the Couette analogy methodology is used to establish the above equivalence in the case of two complex, commercial geometries, namely, a double helical ribbon tool and a square-shaped stirrer, which are recommended for the study of relatively large size suspensions. The methodology is based on the concept of the reduction of the complex geometry to an equivalent coaxial cylinder geometry, thus determining a quantitative correspondence between the non-standard situation and the well-known Couette-like conditions. The Couette analogy has been used first to determine the calibration constants of the non-standard geometry by using a Newtonian oil of known viscosity. The constants have been subsequently used to determine the viscosity curves of two non-Newtonian, shear thinning fluids, namely a homogeneous polymer solution and two heterogeneous concentrated suspensions. The results show that the procedure yields a good agreement between the viscosity curves obtained by the reduction method and those measured by a standard viscometric Couette geometry. The calibration constants obtained in this work from the coaxial cylinder analogy are also compared with those provided by the manufacturer, indicating that the calibration can improve the accuracy of the rheometer output.
Segmentation of Polish Households Taking into Account Food Waste
Authors: Bilska Beata , Tomaszewska Marzena , Kołożyn-Krajewska Danuta , Piecek Małgorzata
Published: April 2020

Currently, food waste is estimated at more than one-third of all food produced, and the primary responsibility for this phenomenon is attributed to households. Therefore, it seems reasonable to take action to limit food waste and to raise awareness about this link in the chain. To develop and implement educational programs addressed at consumers it is necessary to understand the factors determining food waste in households. Segmentation is a tool that can help effectively reach consumers who are to the greatest extent wasting food which identifies homogeneous clusters of consumers. The aim of this study was to perform segmentation to identify consumer groups with similar behaviors in relation to food, with particular emphasis on food wastage. We carried out segmentation on a representative sample of Polish people over 18 years of age and to identified three clusters of consumers. The three consumer segments diagnosed differed in sociodemographic terms, i.e., number of adults, number of children, subjective assessment of the financial situation, and percentage of spending on food. The segment exhibiting a high frequency of discarding food due to too large package size included single and double households.
Induction of Terminal Oxidases of Electron Transport Chain in Broccoli Heads under Controlled Atmosphere Storage
Authors: Makino Yoshio , Inoue Jun , Wang Hsiao-Wen , Yoshimura Masatoshi , Maejima Kensaku , Funayama-Noguchi Sachiko , Yamada Takeshi , Noguchi Ko
Published: April 2020

Controlled atmosphere (CA) storage, that is, at low O2 and high CO2 concentrations, effectively extends the shelf life of horticultural products. The influence of CA storage (O2/CO2: 2.5%/6.0% or 2.5%/0.0%) and in normal air (both at 1 °C for 21 d) on the physicochemical (O2 uptake, mass loss and L-ascorbate) and biological properties of broccoli (Brassica oleracea var. italica, Plenck, 1794) via amounts and activities of terminal oxidases of the electron transport chain was investigated. Mass loss, a sensitive index of freshness for broccoli heads under CA, was significantly lower under CA than under normoxia (p < 0.05). Mass loss was depressed 7 d earlier under CA, including 6.0% CO2 than under CA without CO2. High CO2 effectively depressed the degradation of L-ascorbate. During storage, the activity of the alternative oxidase (AOX) was lower under CA than in normal air (p < 0.05), while the amount of cytochrome c oxidase (COX), and the AOX/COX activity ratio (based on oxygen isotope discrimination), were not affected during storage. Our results indicate that CA storage effectively retained the freshness of broccoli heads by depressing the induction of AOX. However, depression of AOX amount was not associated with CO2 around broccoli heads.
Degree of Hydrolysis Affects the Techno-Functional Properties of Lesser Mealworm Protein Hydrolysates
Authors: Leni Giulia , Soetemans Lise , Caligiani Augusta , Sforza Stefano , Bastiaens Leen
Published: April 2020

Protein hydrolysates from lesser mealworm (Alphitobius diaperinus, LM) were obtained by enzymatic hydrolysis with protease from Bacillus licheniformis. A preliminary test performed for five hours of hydrolysis generated an insect protein hydrolysate with 15% of degree of hydrolysis (DH), optimum solubility property and oil holding capacity, but emulsifying and foaming ability were completely impaired. In order to investigate the potential implication of DH on techno-functional properties, a set of protein hydrolysates with a different DH was obtained by sub-sampling at different time points during three hours of enzymatic hydrolysis process. An increase in DH% had positive effects on the solubility property and oil holding ability, while a reduced emulsifying ability was observed up to five hours of hydrolysis. These results demonstrated that the enzymatic hydrolysis, if performed under controlled conditions and not for a long period, represents a valid method to extract high quality protein from insects with tailored techno-functionality, in order to produce tailored ingredients for feed and food purpose.
Insects as Novel Food: A Consumer Attitude Analysis through the Dominance-Based Rough Set Approach
Authors: Roma Rocco , Ottomano Palmisano Giovanni , De Boni Annalisa
Published: April 2020

In Western societies, the unfamiliarity with insect-based food is a hindrance for consumption and market development. This may depend on neophobia and reactions of disgust, individual characteristics and socio-cultural background, and risk-perceptions for health and production technologies. In addition, in many European countries, the sale of insects for human consumption is still illegal, although European Union (EU) and the European Food Safety Authority (EFSA) are developing regulatory frameworks and environmental and quality standards. This research aims to advance the knowledge on entomophagy, providing insights to improve consumer acceptance in Italy. This is done by carrying out the characterization of a sample of consumers according to their willingness to taste several types of insect-based food and taking into account the connections among the consumers’ features. Thus, the dominance-based rough set approach is applied using the data collected from 310 Italian consumers. This approach provided 206 certain decision rules characterizing the consumers into five groups, showing the consumers’ features determining their specific classification. Although many Italian consumers are willing to accept only insects in the form of feed stuffs or supplements, this choice is a first step towards entomophagy. Conversely, young Italian people are a niche market, but they can play a role in changing trends.
Nutritional Composition of Apis mellifera Drones from Korea and Denmark as a Potential Sustainable Alternative Food Source: Comparison Between Developmental Stages
Authors: Ghosh Sampat , Sohn Ho-Yong , Pyo Su-Jin , Jensen Annette Bruun , Meyer-Rochow Victor Benno , Jung Chuleui
Published: April 2020

We compared nutrient compositions of honey bee (Apis mellifera) drones of different developmental stages from two different populations—the Italian honey bee reared in Korea and Buckfast bees from Denmark. Analyses included amino acid, fatty acid, and mineral content as well as evaluations of antioxidant properties and haemolysis activities. The compositions of total amino acids, and thus protein content of the insects, increased with development. A similar trend was observed for minerals presumably due to the consumption of food in the adult stage. In contrast, total fatty acid amounts decreased with development. Altogether, seventeen amino acids, including all the essential ones, except tryptophan, were determined. Saturated fatty acids dominated over monounsaturated fatty acids in the pupae, but the reverse held true for the adults. Drones were found to be rich in minerals and the particularly high iron as well as K/Na ratio was indicative of the nutritional value of these insects. Among the three developmental stages, adult Buckfast drones exhibited the highest antioxidant activity. Bearing in mind the overall high nutritional value, i.e., high amino acids, minerals and less fatty acids, late pupae and adult drones can be useful for human consumption while the larvae or early pupal stage can be recommended as feed. However, owing to their relatively high haemolysis activity, we advocate processing prior to the consumption of these insects.
Effect of Alternative Protein Feeds on the Content of Selected Endogenous Bioactive and Flavour-Related Compounds in Chicken Breast Meat
Authors: Gkarane Vasiliki , Ciulu Marco , Altmann Brianne , Mörlein Daniel
Published: April 2020

Currently, soybean meal constitutes the main protein source for poultry production. However, the environmental and social issues related to soybean production are calling for more sustainable protein sources that can offset soybean requirements in animal production. Hermetia illucens larvae and the microalga spirulina (Arthrospira platensis) have proven to be effective alternatives to soybean meal for poultry production. In this study, the effect of 100% replacement of soy with partially defatted Hermetia illucens larvae and spirulina on the contents of selected endogenous bioactive (anserine, creatine and carnosine) and flavour-related (inosine and inosine-5´-monophosphate, IMP) compounds in chicken breast meat was evaluated. The results showed that the spirulina-based diet lowered the levels of anserine, carnosine and creatine compared to the control diet (3.3 vs. 4.1 mg/g, 0.15 vs. 0.72 mg/g and 1.49 vs. 2.49 mg/g, respectively) while IMP levels tended to be higher in spirulina-fed samples. Compared to the control group, Hermetia illucens-fed samples showed a lower content of bioactive peptides (anserine: 3.6 vs. 4.1 mg/g; carnosine: 0.39 vs. 0.72 mg/g; creatine: 2.03 vs. 2.49 mg/g), albeit to a lesser extent than the spirulina treatment group.
Extraction Strategies to Recover Bioactive Compounds, Incorporation into Food and Health Benefits: Current Works and Future Challenges
Authors: Contreras María del Mar , Castro Eulogio
Published: April 2020

There are numerous studies in the literature about bioactive products (extracts, essential oils, oleoresins, hydrolysates, etc [...]
Analysis of the Anthocyanin Degradation in Blue Honeysuckle Berry under Microwave Assisted Foam-Mat Drying
Authors: Sun Yu , Zhang Yuhan , Xu Wei , Zheng Xianzhe
Published: April 2020

Changes in nutrient content and bioactivity are important indicators to evaluate the quality of products. Berries are rich in antioxidant anthocyanins, which are prone to degradation during drying. The effects of different variables on the stability of anthocyanins in berry puree during microwave assisted foam-mat drying (MFD) was investigated by path analysis and degradation kinetics analysis. The experimental results showed that the degradation of anthocyanins mainly occurred in the last drying stage. The temperature and the moisture content have both direct and indirect effects on the anthocyanin stability. The direct path coefficient of the moisture content on anthocyanins was 0.985, and the direct path coefficient of temperature on anthocyanins was −0.933. The moisture content to temperature ratio (M/T) was first put forward to estimate the anthocyanin degradation. The results of the regression analysis confirmed that the anthocyanins were stable at M/T of 0.96–3.60. A finite element simulation model was established to predict the anthocyanin degradation rate and content. These research results could provide a theoretical reference for use in optimizing the MFD processing technologies.
Anti-Thrombotic, Anti-Oxidant and Haemolysis Activities of Six Edible Insect Species
Authors: Pyo Su-Jin , Kang Deok-Gyeong , Jung Chuleui , Sohn Ho-Yong
Published: April 2020

In Korea, various insect species such as crickets and grasshoppers, as well as honey bee and silkworm pupae, have been consumed as food and used in oriental medicine. In this study to evaluate useful the bioactivities and potentially adverse effects of edible insects, ethanol extracts of Allomyrina dichotoma (AD), Tenebrio molitor (TM), Protaetia brevitarsis (PB), Gryllus bimaculatus (GB), Teleogryllus emma (TE), and Apis mellifera (AM) were prepared and evaluated with regard to their anti-thrombosis, anti-oxidant and haemolysis activities against human red blood cells. AD and TE extracts showed strong anti-oxidant activities, which were not related to polyphenol content. All ethanol extracts, except AM extract, showed strong platelet aggregation activities. The platelet aggregation ratios of the extracts were 194%–246% of those of the solvent controls. The effects of the AD, TM, PB, GM, and AM extracts on thrombin, prothrombin and various coagulation factors were negligible. Only the extract of TM showed concentration-dependent anti-coagulation activities, with a 1.75-fold aPTT (activated Partial Thromboplastin Time) extension at 5 mg/mL. Of the six insect extracts, TM and AM extracts exhibited potent haemolytic activity. Our results on the insect extracts’ functional properties suggest that edible insects have considerable potential not just as a food source but as a novel bio-resource as well.
“My Daddy … He Was a Good Man”: Gendered Genealogies and Memories of Enslaved Fatherhood in America’s Antebellum South
Authors: Grant Susan-Mary , Bowe David
Published: April 2020

While the last few years have witnessed an upsurge of studies into enslaved motherhood in the antebellum American South, the role of the enslaved father remains largely trapped within a paradigm of enforced absenteeism from an unstable and insecure familial unit. The origins of this lie in the racist assumptions of the infamous “Moynihan Report” of 1965, read backwards into slavery itself. Consequently, the historiographical trajectory of work on enslaved men has drawn out the performative aspects of their masculinity in almost every area of their lives except that of fatherhood. This has produced an image of individualistic masculinity, separate from the familial role that many enslaved men managed to sustain and, as a result, productive of a disjointed and gendered genealogy of slavery and its legacy. This paper assesses the extent to which this fractured genealogy actually represents the former slaves’ worldview. By examining a selection of interviews conducted by the Federal Writers’ Project under the auspices of the Works Progress Administration (WPA) in the 1930s (the WPA Narratives), this paper explores formers slaves’ memories of their enslaved fathers and the significance of the voluntary paternal presence in their life stories. It concludes that the role of the black father was of greater significance than so far recognised by the genealogical narratives that emerged from the slave communities of the Antebellum South.
A Whole Genome Re-Sequencing Based GWA Analysis Reveals Candidate Genes Associated with Ivermectin Resistance in Haemonchus contortus
Authors: Khan Sawar , Nisar Ayesha , Yuan Jianqi , Luo Xiaoping , Dou Xueqin , Liu Fei , Zhao Xiaochao , Li Junyan , Ahmad Habib , Mehmood Sardar Azhar , Feng Xingang
Published: April 2020

The most important and broad-spectrum drug used to control the parasitic worms to date is ivermectin (IVM). Resistance against IVM has emerged in parasites, and preserving its efficacy is now becoming a serious issue. The parasitic nematode Haemonchus contortus (Rudolphi, 1803) is economically an important parasite of small ruminants across the globe, which has a successful track record in IVM resistance. There are growing evidences regarding the multigenic nature of IVM resistance, and although some genes have been proposed as candidates of IVM resistance using lower magnification of genome, the genetic basis of IVM resistance still remains poorly resolved. Using the full magnification of genome, we herein applied a population genomics approach to characterize genome-wide signatures of selection among pooled worms from two susceptible and six ivermectin-resistant isolates of H. contortus, and revealed candidate genes under selection in relation to IVM resistance. These candidates also included a previously known IVM-resistance-associated candidate gene HCON_00148840, glc-3. Finally, an RNA-interference-based functional validation assay revealed the HCON_00143950 as IVM-tolerance-associated gene in H. contortus. The possible role of this gene in IVM resistance could be detoxification of xenobiotic in phase I of xenobiotic metabolism. The results of this study further enhance our understanding on the IVM resistance and continue to provide further evidence in favor of multigenic nature of IVM resistance.
Use of Clinical Preventive Service and Related Factors in Middle-Aged Postmenopausal Women in Korea
Authors: Kim Kisook
Published: April 2020

Postmenopausal women experiencing health transitions can improve health-related quality of life through clinical health service use. The aim of this study was to investigate the factors affecting clinical preventive service use, focusing on a multi-dimensional approach among middle-aged postmenopausal women. This descriptive study is a secondary analysis of the seventh Korea National Health and Nutrition Examination Survey (KNHANESVII-1) in 2016. Among the 8150 participants, our analysis included 771 naturally menopausal women aged 40–65. National health insurance (OR = 1.659, 95% CI = 1.080–2.550), private health insurance (OR = 2.877, 95% CI = 1.665–4.971), needs for health service (OR = 2.363, 95% CI = 1.332–4.195), cardiovascular disease (OR = 1.570, 95% CI = 1.009–2.445), hospital admission (OR = 3.054, 95% CI = 1.298–7.184), smoking (OR = 0.262, 95% CI = 0.144–0.477), drinking (OR = 0.573, 95% CI = 0.335–0.979), and depression (OR = 0.535, 95% CI = 0.340–0.841) were associated with clinical preventive service use among middle-aged postmenopausal women. To promote clinical preventive service use among postmenopausal women, policies promoting health behavior expansion should be introduced and should consider the predictive variables revealed by this study.
Sulphur Dioxide Pads Can Reduce Gray Mold While Maintaining the Quality of Clamshell-Packaged ‘BRS Nubia’ Seeded Table Grapes Grown under Protected Cultivation
Authors: Youssef Khamis , Junior Osmar Jose Chaves , Mühlbeier Débora Thaís , Roberto Sergio Ruffo
Published: April 2020

The purpose of this research is to test the efficacy of different types of SO2-generating pads on the incidence of gray mold, and on the physicochemical properties of quality of ‘BRS Nubia’ seeded table grapes grown under protected cultivation. Four types of SO2-generating pads, 5 or 8 g of sodium metabisulfite dual release pads, and 4 or 7 g of sodium metabisulfite slow release pads, were used. Grapes bunches were harvested from a vineyard covered with plastic mash and stored in a cold room at 1 ± 1 °C for 45 days followed by 6 days of shelf life at 22 ± 1 °C at a high relative humidity (>95%). The results showed that SO2-generating pads with a dual release of 5 or 8 g completely inhibited the development of gray mold at all evaluation times. Also, a high reduction of the disease incidence was achieved by using a slow release of 4 g. The study confirmed that SO2-generating pads did not alter the physicochemical properties of ‘BRS Nubia’ seeded table grapes including mass loss, berry firmness, color index, total anthocyanin concentration, total soluble solids (TSS), titratable acidity (TA), and the TSS/TA ratio. Slow release pads at 4 and 7 g reduced the percentage of shattered berries by 56 and 48% as compared to control only after 6 days of shelf life. Also, all types of SO2-generating pads reduced the stem browning score at the end of cold storage. The 5 or 8 g dual release pads and 4 g slow release pads can be considered for effective controlling of gray mold for ‘BRS Nubia’ table grapes grown under protected cultivation while maintaining grape quality.
Influence of Skull Fracture on Traumatic Brain Injury Risk Induced by Blunt Impact
Authors: Ren Lihai , Wang Dangdang , Liu Xi , Yu Huili , Jiang Chengyue , Hu Yuanzhi
Published: April 2020

This study is aimed at investigating the influence of skull fractures on traumatic brain injury induced by blunt impact via numerous studies of head–ground impacts. First, finite element (FE) damage modeling was implemented in the skull of the Total HUman Model for Safety (THUMS), and the skull fracture prediction performance was validated against a head–ground impact experiment. Then, the original head model of the THUMS was assigned as the control model without skull element damage modeling. Eighteen (18) head–ground impact models were established using these two FE head models, with three head impact locations (frontal, parietal, and occipital regions) and three impact velocities (25, 35, and 45 km/h). The predicted maximum principal strain and cumulative strain damage measure of the brain tissue were employed to evaluate the effect of skull fracture on the cerebral contusion and diffuse brain injury risks, respectively. Simulation results showed that the skull fracture could reduce the risk of diffuse brain injury risk under medium and high velocities significantly, while it could increase the risk of brain contusion under high-impact velocity.
Regulative Loop between β-catenin and Protein Tyrosine Receptor Type γ in Chronic Myeloid Leukemia
Authors: Tomasello Luisa , Vezzalini Marzia , Boni Christian , Bonifacio Massimiliano , Scaffidi Luigi , Yassin Mohamed , Al-Dewik Nader , Takam Kamga Paul , Krampera Mauro , Sorio Claudio
Published: April 2020

Protein tyrosine phosphatase receptor type γ (PTPRG) is a tumor suppressor gene, down-regulated in Chronic Myeloid Leukemia (CML) cells by the hypermethylation of its promoter region. β-catenin (CTNNB1) is a critical regulator of Leukemic Stem Cells (LSC) maintenance and CML proliferation. This study aims to demonstrate the antagonistic regulation between β-catenin and PTPRG in CML cells. The specific inhibition of PTPRG increases the activation state of BCR-ABL1 and modulates the expression of the BCR-ABL1- downstream gene β-Catenin. PTPRG was found to be capable of dephosphorylating β-catenin, eventually causing its cytosolic destabilization and degradation in cells expressing PTPRG. Furthermore, we demonstrated that the increased expression of β-catenin in PTPRG-negative CML cell lines correlates with DNA (cytosine-5)-methyl transferase 1 (DNMT1) over-expression, which is responsible for PTPRG promoter hypermethylation, while its inhibition or down-regulation correlates with PTPRG re-expression. We finally confirmed the role of PTPRG in regulating BCR-ABL1 and β-catenin phosphorylation in primary human CML samples. We describe here, for the first time, the existence of a regulative loop occurring between PTPRG and β-catenin, whose reciprocal imbalance affects the proliferation kinetics of CML cells.
Novel Insight into Neuroimmune Regulatory Mechanisms and Biomarkers Linking Major Depression and Vascular Diseases: The Dilemma Continues
Authors: Tonhajzerova Ingrid , Sekaninova Nikola , Bona Olexova Lucia , Visnovcova Zuzana
Published: April 2020

Major depressive disorder (MDD) represents a serious health problem estimated to affect 350 million people globally. Importantly, MDD has repeatedly emerged as an etiological or prognostic factor in cardiovascular disease (CVD) development, including vascular pathology. Several linking pathomechanisms between MDD and CVD involve abnormal autonomic regulation, inflammation, and endothelial dysfunction as an early preclinical stage of atherosclerosis. However, the cause of accelerated atherosclerosis in MDD patients remains unclear. Recently, the causal relationships between MDD and mediator (e.g., inflammation and/or endothelial dysfunction), as well as the causal pathways from the mediator to atherosclerosis, were discussed. Specifically, MDD is accompanied by immune dysregulation, resulting in increased production of proinflammatory cytokines (e.g., interleukin (IL)-6 and tumor necrosis factor (TNF)-α), which could lead to depression-linked abnormalities in brain function. Further, MDD has an adverse effect on endothelial function; for example, circulating markers of endothelial dysfunction (e.g., soluble adhesion molecules, von Willebrand factor) have been linked with depression. Additionally, MDD-linked autonomic dysregulation, which is characterized by disrupted sympathovagal balance associated with excessive circulating catecholamines, can contribute to CVD. Taken together, activated inflammatory response, endothelial dysfunction, and autonomic dysregulation could affect gradual atherosclerosis progression, resulting in a higher risk of developing CVD in MDD. This review focused on the pathomechanisms linking MDD and CVD with respect to neuroimmune regulation, and the description of promising biomarkers, which is important for the early diagnosis and personalized prevention of CVD in major depression.
Using Phosphatidylinositol Phosphorylation as Markers for Hyperglycemic Related Breast Cancer
Authors: Devanathan Nirupama , Jones Sandra , Kaur Gursimran , Kimble-Hill Ann C.
Published: April 2020

Studies have suggested that type 2 diabetes (T2D) is associated with a higher incidence of breast cancer and related mortality rates. T2D postmenopausal women have an ~20% increased chance of developing breast cancer, and women with T2D and breast cancer have a 50% increase in mortality compared to breast cancer patients without diabetes. This correlation has been attributed to the general activation of insulin receptor signaling, glucose metabolism, phosphatidylinositol (PI) kinases, and growth pathways. Furthermore, the presence of breast cancer specific PI kinase and/or phosphatase mutations enhance metastatic breast cancer phenotypes. We hypothesized that each of the breast cancer subtypes may have characteristic PI phosphorylation profiles that are changed in T2D conditions. Therefore, we sought to characterize the PI phosphorylation when equilibrated in normal glycemic versus hyperglycemic serum conditions. Our results suggest that hyperglycemia leads to: 1) A reduction in PI3P and PIP3, with increased PI4P that is later converted to PI(3,4)P2 at the cell surface in hormone receptor positive breast cancer; 2) a reduction in PI3P and PI4P with increased PIP3 surface expression in human epidermal growth factor receptor 2-positive (HER2+) breast cancer; and 3) an increase in di- and tri-phosphorylated PIs due to turnover of PI3P in triple negative breast cancer. This study begins to describe some of the crucial changes in PIs that play a role in T2D related breast cancer incidence and metastasis.
Cancer Cachexia and Related Metabolic Dysfunction
Authors: Fonseca Guilherme Wesley Peixoto da , Farkas Jerneja , Dora Eva , von Haehling Stephan , Lainscak Mitja
Published: April 2020

Cancer cachexia is a complex multifactorial syndrome marked by a continuous depletion of skeletal muscle mass associated, in some cases, with a reduction in fat mass. It is irreversible by nutritional support alone and affects up to 74% of patients with cancer—dependent on the underlying type of cancer—and is associated with physical function impairment, reduced response to cancer-related therapy, and higher mortality. Organs, like muscle, adipose tissue, and liver, play an important role in the progression of cancer cachexia by exacerbating the pro- and anti-inflammatory response initially activated by the tumor and the immune system of the host. Moreover, this metabolic dysfunction is produced by alterations in glucose, lipids, and protein metabolism that, when maintained chronically, may lead to the loss of skeletal muscle and adipose tissue. Although a couple of drugs have yielded positive results in increasing lean body mass with limited impact on physical function, a single therapy has not lead to effective treatment of this condition. Therefore, a multimodal intervention, including pharmacological agents, nutritional support, and physical exercise, may be a reasonable approach for future studies to better understand and prevent the wasting of body compartments in patients with cancer cachexia.
Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches
Authors: Voronin Denis V. , Kozlova Anastasiia A. , Verkhovskii Roman A. , Ermakov Alexey V. , Makarkin Mikhail A. , Inozemtseva Olga A. , Bratashov Daniil N.
Published: April 2020

Flow cytometry nowadays is among the main working instruments in modern biology paving the way for clinics to provide early, quick, and reliable diagnostics of many blood-related diseases. The major problem for clinical applications is the detection of rare pathogenic objects in patient blood. These objects can be circulating tumor cells, very rare during the early stages of cancer development, various microorganisms and parasites in the blood during acute blood infections. All of these rare diagnostic objects can be detected and identified very rapidly to save a patient’s life. This review outlines the main techniques of visualization of rare objects in the blood flow, methods for extraction of such objects from the blood flow for further investigations and new approaches to identify the objects automatically with the modern deep learning methods.
Interspecies Communication in Holobionts by Non-Coding RNA Exchange
Authors: Leitão Ana Lúcia , Costa Marina C. , Gabriel André F. , Enguita Francisco J.
Published: April 2020

Complex organisms are associations of different cells that coexist and collaborate creating a living consortium, the holobiont. The relationships between the holobiont members are essential for proper homeostasis of the organisms, and they are founded on the establishment of complex inter-connections between all the cells. Non-coding RNAs are regulatory molecules that can also act as communication signals between cells, being involved in either homeostasis or dysbiosis of the holobionts. Eukaryotic and prokaryotic cells can transmit signals via non-coding RNAs while using specific extracellular conveyors that travel to the target cell and can be translated into a regulatory response by dedicated molecular machinery. Within holobionts, non-coding RNA regulatory signaling is involved in symbiotic and pathogenic relationships among the cells. This review analyzes current knowledge regarding the role of non-coding RNAs in cell-to-cell communication, with a special focus on the signaling between cells in multi-organism consortia.
Immunotherapy for Uterine Cervical Cancer Using Checkpoint Inhibitors: Future Directions
Authors: Kagabu Masahiro , Nagasawa Takayuki , Sato Chie , Fukagawa Yasuko , Kawamura Hanae , Tomabechi Hidetoshi , Takemoto Shuji , Shoji Tadahiro , Baba Tsukasa
Published: April 2020

Immune checkpoint inhibitors (ICIs) have demonstrated marked clinical effects worldwide, and “cancer immunotherapy” has been recognized as a feasible option for cancer treatment. Significant treatment responses have already been attained for malignant melanoma and lung cancer, ahead of gynecologic cancer. In cervical cancer, however, results are only available from phase II trials, not from phase III trials. Cervical cancer is a malignant tumor and is the fourth most common cancer among women worldwide. Since the introduction of angiogenesis inhibitors, treatment for recurrent and advanced cervical cancers has improved in the past five years, but median overall survival is 16.8 months for advanced cervical cancer, and all-stage five-year overall survival rate is 68%, indicating that treatment effects remain inadequate. For this reason, the development of new therapeutic approaches is imperative. We describe herein the KEYNOTE-158 and CheckMate 358 clinical trials, which were conducted for cervical cancer, and discuss future directions, including potential combinations with concurrent chemoradiation therapy (CCRT), as noted for other types of cancer.
Anti-Epileptogenic Effects of Antiepileptic Drugs
Authors: Miziak Barbara , Konarzewska Agnieszka , Ułamek-Kozioł Marzena , Dudra-Jastrzębska Monika , Pluta Ryszard , Czuczwar Stanisław J.
Published: April 2020

Generally, the prevalence of epilepsy does not exceed 0.9% of the population and approximately 70% of epilepsy patients may be adequately controlled with antiepileptic drugs (AEDs). Moreover, status epilepticus (SE) or even a single seizure may produce neurodegeneration within the brain and SE has been recognized as one of acute brain insults leading to acquired epilepsy via the process of epileptogenesis. Two questions thus arise: (1) Are AEDs able to inhibit SE-induced neurodegeneration? and (2) if so, can a probable neuroprotective potential of particular AEDs stop epileptogenesis? An affirmative answer to the second question would practically point to the preventive potential of a given neuroprotective AED following acute brain insults. The available experimental data indicate that diazepam (at low and high doses), gabapentin, pregabalin, topiramate and valproate exhibited potent or moderate neuroprotective effects in diverse models of SE in rats. However, only diazepam (at high doses), gabapentin and pregabalin exerted some protective activity against acquired epilepsy (spontaneous seizures). As regards valproate, its effects on spontaneous seizures were equivocal. With isobolography, some supra-additive combinations of AEDs have been delineated against experimental seizures. One of such combinations, levetiracetam + topiramate proved highly synergistic in two models of seizures and this particular combination significantly inhibited epileptogenesis in rats following status SE. Importantly, no neuroprotection was evident. It may be strikingly concluded that there is no correlation between neuroprotection and antiepileptogenesis. Probably, preclinically verified combinations of AEDs may be considered for an anti-epileptogenic therapy.
HTR1A, HTR1B, HTR2A, HTR2C and HTR6 Gene Polymorphisms and Extrapyramidal Side Effects in Haloperidol-Treated Patients with Schizophrenia
Authors: Grubor Mirko , Zivkovic Maja , Sagud Marina , Nikolac Perkovic Matea , Mihaljevic-Peles Alma , Pivac Nela , Muck-Seler Dorotea , Svob Strac Dubravka
Published: April 2020

Schizophrenia is a serious, chronic psychiatric disorder requiring lifelong treatment. Extrapyramidal side effects (EPS) are common adverse reactions to antipsychotic medications. In addition to the dopaminergic system, serotonergic mechanisms, including serotonin (5-HT) receptors, might be involved in EPS development. This study aimed to examine molecular associations of HTR1A, HTR1B, HTR2A, HTR2C and HTR6 gene polymorphisms with acute EPS in 229 male schizophrenia patients, following two weeks of haloperidol monotherapy. The Simpson–Angus Rating Scale for Extrapyramidal Side Effects (SAS), Barnes Akathisia Rating Scale (BARS) and Extrapyramidal Symptom Rating Scale (ESRS) were used to evaluate EPS severity. Genotyping was performed using real-time PCR, following extraction of blood DNA. Significant acute EPS appeared in 48.03% of schizophrenia patients. For the rs13212041 HTR1B gene polymorphism, affecting microRNA regulation of HTR1B gene expression, a higher frequency of TT carriers was found among haloperidol-treated patients with akathisia when compared to the group without akathisia symptoms. In comparison to C-allele carriers, patients carrying the TT genotype had higher akathisia severity, as determined by the SAS, BARS and ESRS scales. These molecular findings suggest potential involvement of 5-HT1B receptors in akathisia development following haloperidol treatment, as well as possible epigenetic mechanisms of serotonergic modulation associated with antipsychotic-induced EPS.
Mechanisms behind Retinal Ganglion Cell Loss in Diabetes and Therapeutic Approach
Authors: Potilinski María Constanza , Lorenc Valeria , Perisset Sofía , Gallo Juan Eduardo
Published: April 2020

Diabetes produces several changes in the body triggered by high glycemia. Some of these changes include altered metabolism, structural changes in blood vessels and chronic inflammation. The eye and particularly the retinal ganglion cells (RGCs) are not spared, and the changes eventually lead to cell loss and visual function impairment. Understanding the mechanisms resulting in RGC damage and loss from diabetic retinopathy is essential to find an effective treatment. This review focuses mainly on the signaling pathways and molecules involved in RGC loss and the potential therapeutic approaches for the prevention of this cell death. Throughout the manuscript it became evident that multiple factors of different kind are responsible for RGC damage. This shows that new therapeutic agents targeting several factors at the same time are needed. Alpha-1 antitrypsin as an anti-inflammatory agent may become a suitable option for the treatment of RGC loss because of its beneficial interaction with several signaling pathways involved in RGC injury and inflammation. In conclusion, alpha-1 antitrypsin may become a potential therapeutic agent for the treatment of RGC loss and processes behind diabetic retinopathy.
Histone Deacetylation Inhibitors as Modulators of Regulatory T Cells
Authors: von Knethen Andreas , Heinicke Ulrike , Weigert Andreas , Zacharowski Kai , Brüne Bernhard
Published: April 2020

Regulatory T cells (Tregs) are important mediators of immunological self-tolerance and homeostasis. Being cluster of differentiation 4+Forkhead box protein3+ (CD4+FOXP3+), these cells are a subset of CD4+ T lymphocytes and can originate from the thymus (tTregs) or from the periphery (pTregs). The malfunction of CD4+ Tregs is associated with autoimmune responses such as rheumatoid arthritis (RA), multiple sclerosis (MS), type 1 diabetes (T1D), inflammatory bowel diseases (IBD), psoriasis, systemic lupus erythematosus (SLE), and transplant rejection. Recent evidence supports an opposed role in sepsis. Therefore, maintaining functional Tregs is considered as a therapy regimen to prevent autoimmunity and allograft rejection, whereas blocking Treg differentiation might be favorable in sepsis patients. It has been shown that Tregs can be generated from conventional naïve T cells, called iTregs, due to their induced differentiation. Moreover, Tregs can be effectively expanded in vitro based on blood-derived tTregs. Taking into consideration that the suppressive role of Tregs has been mainly attributed to the expression and function of the transcription factor Foxp3, modulating its expression and binding to the promoter regions of target genes by altering the chromatin histone acetylation state may turn out beneficial. Hence, we discuss the role of histone deacetylation inhibitors as epigenetic modulators of Tregs in this review in detail.