bluuebunny/crossref_metadata_2025 huggingface dataset:
Accuracy of a portable accelerometer-based navigation system for cup placement and intraoperative leg length measurement in total hip arthroplasty: a cross-sectional study
Authors: Tanino Hiromasa , Nishida Yasuhiro , Mitsutake Ryo , Ito Hiroshi
Published: March 2021

Background

Complications after total hip arthroplasty (THA) are frequently the consequence of malpositioned components or leg length discrepancy after surgery. Recently, a new version of a portable, accelerometer-based hip navigation system (New HipAlign) was made available with a change in the method of measuring cup abduction and the addition of a leg length measurement function. The purposes of this study were to investigate cup positioning and to examine the accuracy of leg length measurement with New HipAlign.

Methods

Cups were implanted and intraoperative leg length change was measured using New HipAlign in 60 THAs through a posterior approach in the lateral decubitus position. The cup position and radiographic leg length change were determined postoperatively on pelvic radiograph and computed tomography scans. We previously compared cup positioning with a previous version of a portable, accelerometer-based hip navigation system (Previous HipAlign) and conventional surgical techniques. Cup positioning in this study was compared with the results of out previous study using Previous HipAlign.

Results

The mean cup abduction of 40.3° ± 4.9° (range, 26° to 53°) and the mean cup anteversion of 15.8° ± 5.6° (range, 6.7° to 29.5°) were found. The deviation of the postoperative measured angles from the target cup position was 3.7° ± 3.3° for cup abduction and 5.9° ± 3.6° for cup anteversion. 56/60 of the cups were inside the Lewinnek safe zone. Compared with our previous study using Previous HipAlign, there were no significant differences with regard to cup abduction, cup anteversion, the deviation from the target cup position for cup abduction, the value of deviation for cup anteversion, and the number of cups inside the Lewinnek safe zone (P = 0.218, 0.334, 0.651, 0.797, 0.592). The mean difference between the intraoperative and radiographic leg length changes was + 0.8 ± 3.4 mm. There was significant correlation between the intraoperative and radiographic leg length changes (r = 0.804,P = 0.000).

Conclusions

Use of New HipAlign allowed for accurate cup placement and reliable leg length measurement during THA.

Trial registration

Clinical trial is defined as‘any research study that prospectively assigns human participants or groups of humans to one or more health-related interventions to evaluate the effects on health outcome’ bythe World Health Organization (WHO).Because this study is not a clinical trial, trial registration is not needed.
In vitro assessment of two novel Cellulases from Trabulsiella odontotermitis for agricultural waste utilization
Authors: Arevalos-Sánchez Martha María , Maynez-Perez Adrián Omar , Rodríguez-Almeida Felipe A. , Martínez-Quintana José Alfredo , Sanchez-Flores Fidel Alejandro , Felix-Portillo Monserrath , Chavéz-Martínez América , Olvera-García Myrna Elena , Ruiz-Barrera Oscar , Corral-Luna Agustín
Published: March 2021

Background

The production of agricultural wastes still growing as a consequence of the population growing. However, the majority of these residues are under-utilized due their chemical composition, which is mainly composed by cellulose. Actually, the search of cellulases with high efficiency to degrade this carbohydrate remains as the challenge. In the present experiment, two genes encoding an endoglucanase (EC 3.2.1.4) and β-glucosidase (EC 3.2.1.21) were overexpressed in Escherichia coli and their recombinant enzymes (egl-FZYE and cel-FZYE, respectively) characterized. Those genes were found in Trabulsiella odontermitis which was isolated from the gut of termite Heterotermes sp. Additionally, the capability to release sugars from agricultural wastes was evaluated in both enzymes, alone and in combination.

Results

The results have shown that optimal pH was 6.0 and 6.5, reaching an activity of 1051.65 ± 47.78 and 607.80 ± 10.19 U/mg at 39 °C, for egl-FZYE and cel-FZYE, respectively. The Km and Vmax for egl-FZYE using CMC as substrate were 11.25 mg/mL and 3921.57 U/mg, respectively, whereas using Avicel were 15.39 mg/mL and 2314.81 U/mg, respectively. The Km and Vmax for cel-FZYE using Avicel as substrate were 11.49 mg/mL and 2105.26 U/mg, respectively, whereas using CMC the enzyme did not had activity. Both enzymes had effect on agricultural wastes, and their effect was improved when they were combined reaching an activity of 955.1 ± 116.1, 4016.8 ± 332 and 1124.2 ± 241 U/mg on corn stover, sorghum stover and pine sawdust, respectively.

Conclusions

Both enzymes were capable of degrading agricultural wastes, and their effectiveness was improved up to 60% of glucose released when combined. In summary, the results of the study demonstrate that the recombinant enzymes exhibit characteristics that indicate their value as potential feed additives and that the enzymes could be used to enhance the degradation of cellulose in the poor-quality forage generally used in ruminant feedstuffs.
Efficacy and safety of single-inhaler extrafine triple therapy versus inhaled corticosteroid plus long-acting beta2 agonist in eastern Asian patients with COPD: the TRIVERSYTI randomised controlled trial
Authors: Zheng Jinping , Baldi Simonetta , Zhao Li , Li Huiping , Lee Kwan-Ho , Singh Dave , Papi Alberto , Grapin Frédérique , Guasconi Alessandro , Georges George
Published: March 2021

Background

A single-inhaler extrafine triple combination of beclometasone dipropionate (BDP), formoterol fumarate (FF) and glycopyrronium (G) has been developed for maintenance therapy of chronic obstructive pulmonary disease (COPD). This study evaluated the efficacy and safety of BDP/FF/G in patients in three eastern Asian areas: China, Republic of Korea and Taiwan.

Methods

TRIVERSYTI was a double-blind, randomised, active-controlled, parallel-group study in patients with COPD, post-bronchodilator forced expiratory volume in 1 s (FEV1) < 50% predicted, ≥ 1 exacerbation in the previous 12 months, and receiving inhaled maintenance medication. Patients received either extrafine BDP/FF/G 100/6/10 µg via pressurised metered-dose inhaler, or non-extrafine budesonide/formoterol (BUD/FF) 160/4.5 µg via dry-powder inhaler, both administered as two puffs twice-daily for 24 weeks. The co-primary objectives (analysed in the overall population) were to demonstrate superiority of BDP/FF/G over BUD/FF for change from baseline in pre-dose morning and 2-h post-dose FEV1 at Week 24 (these were analysed as key secondary objectives in the China subgroup). The rate of moderate/severe COPD exacerbations was a secondary endpoint.

Results

Of 708 patients randomised, 88.8% completed. BDP/FF/G was superior to BUD/FF for pre-dose and 2-h post-dose FEV1 at Week 24 [adjusted mean differences 62 (95% CI 38, 85) mL and 113 (87, 140) mL; both p < 0.001]. The annualised moderate/severe exacerbation rate was 43% lower with BDP/FF/G [rate ratio 0.57 (95% CI 0.42, 0.77); p < 0.001]. Adverse events were reported by 61.1% and 67.0% patients with BDP/FF/G and BUD/FF. Results were similar in the China subgroup.

Conclusions

In patients with COPD, FEV1 < 50% and an exacerbation history despite maintenance therapy, treatment with extrafine BDP/FF/G improved bronchodilation, and was more effective at preventing moderate/severe COPD exacerbations than BUD/FF.

Trial registration CFDA CTR20160507 (registered 7 Nov 2016, http://www.chinadrugtrials.org.cn/index.html).
A retail audit of mosquito control products in Busia County, western Kenya
Authors: Oria Prisca A. , Moshi Vincent , Odero Julius I. , Ekodir Sheila , Monroe April , Harvey Steven A. , Ochomo Eric , Piccinini Black Danielle
Published: March 2021

Background

Approximately 70% of Kenya’s population is at risk for malaria. The core vector control methods in Kenya are insecticide-treated mosquito nets (ITNs) and indoor residual spraying, with supplementary larval source management. In 2015, 21% of ITNs were accessed through the private retail sector. Despite the private sector role in supplying mosquito control products (MCPs), there is little evidence on the availability, sales trends, and consumer preferences for MCPs other than ITNs. This study, a component of a larger research programme focused on evaluating a spatial repellent intervention class for mosquito-borne disease control, addressed this evidence gap on the role of the private sector in supplying MCPs.

Methods

A cross-sectional survey was deployed in a range of retail outlets in Busia County to characterize MCP availability, sales trends, and distribution channels. The questionnaire included 32 closed-ended and four open-ended questions with short answer responses. Descriptive analysis of frequency counts and percentages was carried out to glean insights about commercially available MCPs and the weighted average rank was used to determine consumer preferences for MCPs. Open-ended data was analysed thematically.

Results

Retail outlets that stocked MCPs commonly stocked mosquito coils (73.0%), topical repellents (38.1%), aerosol insecticide sprays (23.8%) and ITNs (14.3%). Overall, retailers reported the profits from selling MCPs were adequate and they overwhelmingly planned to continue stocking the products. Of respondents who stocked MCPs, 96.8% responded that sales increased during long rains and 36.5% that sales also surged during short rains. ITNs and baby-size nets were often delivered by the wholesaler. Retailers of aerosol sprays, mosquito coils, and topical repellents either collected stock from the wholesaler or products were delivered to them. Other commercially available MCPs included insecticide incense sticks, electric mosquito strikers, insecticide soaps, electrically heated insecticide mats, and electric insecticide emanators, indicating a well-established market.

Conclusions

The wide range of MCPs in local retail outlets within the study area suggests the need and demand for mosquito control tools, in addition to ITNs, that are affordable, easy to use and effective. The presence of a wide range of MCPs, is a promising sign for the introduction of a spatial repellent intervention class of products that meets consumer needs and preferences.
Daily emotional inertia and long-term subjective well-being among people living with HIV
Authors: Rzeszutek Marcin , Gruszczyńska Ewa , Firląg-Burkacka Ewa
Published: March 2021

Objective

The aim of this study was to verify if subjective well-being (SWB) modifies the autoregressive effect of daily emotions and if this emotional inertia predicts long-term changes in SWB among people living with HIV (PLWH).

Methods

The 131 participants had medically confirmed diagnoses of HIV and were undergoing antiretroviral therapy. They assessed their SWB (satisfaction with life, negative affect, positive affect) twice with an interval of one year. They also took part in a five-day online diary study six months from their baseline SWB assessment and reported their daily negative and positive emotions.

Results

Results showed that baseline SWB did not modify the emotional carryover effect from one to another. Additionally, after control for baseline SWB, emotional inertia did not predict SWB one year later. However, such an effect was noted for the mean values of daily reported emotions, indicating their unique predictive power over SWB itself.

Conclusions

This may suggest that emotional inertia does not necessarily provide better information than more straightforward measures of affective functioning.
Association of exacerbation phenotype with the sputum microbiome in chronic obstructive pulmonary disease patients during the clinically stable state
Authors: Yang Chia-Yu , Li Shiao-Wen , Chin Chia-Yin , Hsu Chia-Wei , Lee Chi-Ching , Yeh Yuan-Ming , Wu Kuo-An
Published: March 2021

Background

Chronic obstructive pulmonary disease (COPD) is a progressive, life-threatening lung disease with increasing prevalence and incidence worldwide. Increasing evidence suggests that lung microbiomes might play a physiological role in acute exacerbations of COPD. The objective of this study was to characterize the association of the microbiota and exacerbation risk or airflow limitation in stable COPD patients.

Methods

The sputum microbiota from 78 COPD outpatients during periods of clinical stability was investigated using 16S rRNA V3-V4 amplicon sequencing. The microbiome profiles were compared between patients with different risks of exacerbation, i.e., the low risk exacerbator (LRE) or high risk exacerbator (HRE) groups, and with different airflow limitation severity, i.e., mild to moderate (FEV1 ≥ 50; PFT I) or severe to very severe (FEV1 < 50; PFT II).

Results

The bacterial diversity (Chao1 and observed OTUs) was significantly decreased in the HRE group compared to that in the LRE group. The top 3 dominant phyla in sputum were Firmicutes, Actinobacteria, and Proteobacteria, which were similar in the HRE and LRE groups. At the genus level, compared to that in the LRE group (41.24%), the proportion of Streptococcus was slightly decreased in the HRE group (28.68%) (p = 0.007). However, the bacterial diversity and the proportion of dominant bacteria at the phylum and genus levels were similar between the PFT I and PFT II groups. Furthermore, the relative abundances of Gemella morbillorum, Prevotella histicola, and Streptococcus gordonii were decreased in the HRE group compared to those in the LRE group according to linear discriminant analysis effect size (LEfSe). Microbiome network analysis suggested altered bacterial cooperative regulation in different exacerbation phenotypes. The proportions of Proteobacteria and Neisseria were negatively correlated with the FEV1/FVC value. According to functional prediction of sputum bacterial communities through Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis, genes involved in lipopolysaccharide biosynthesis and energy metabolism were enriched in the HRE group.

Conclusion

The present study revealed that the sputum microbiome changed in COPD patients with different risks of exacerbation. Additionally, the bacterial cooperative networks were altered in the HRE patients and may contribute to disease exacerbation. Our results provide evidence that sputum microbiome community dysbiosis is associated with different COPD phenotypes, and we hope that by understanding the lung microbiome, a potentially modifiable clinical factor, further targets for improved COPD therapies during the clinically stable state may be elucidated.
HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) versus adult T-cell leukemia/lymphoma (ATLL)
Authors: Zarei-Ghobadi Mohadeseh , Sheikhi Mohsen , Teymoori-Rad Majid , Yaslianifard Sahar , Norouzi Mehdi , Yaslianifard Somayeh , Faraji Reza , Farahmand Mohammad , Bayat Shiva , Jafari Mohieddin , Mozhgani Sayed-Hamidreza
Published: March 2021

Objectives

Human T cell leukemia virus-1 (HTLV-1) infection may lead to one or both diseases including HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) or adult T cell leukemia lymphoma (ATLL). The complete interactions of the virus with host cells in both diseases is yet to be determined. This study aims to construct an interaction network for distinct signaling pathways in these diseases based on finding differentially expressed genes (DEGs) between HAM/TSP and ATLL.

Results

We identified 57 hub genes with higher criteria scores in the primary protein–protein interaction network (PPIN). The ontology-based enrichment analysis revealed following important terms: positive regulation of transcription from RNA polymerase II promoter, positive regulation of transcription from RNA polymerase II promoter involved in meiotic cell cycle and positive regulation of transcription from RNA polymerase II promoter by histone modification. The upregulated genes TNF, PIK3R1, HGF, NFKBIA, CTNNB1, ESR1, SMAD2, PPARG and downregulated genes VEGFA, TLR2, STAT3, TLR4, TP53, CHUK, SERPINE1, CREB1 and BRCA1 were commonly observed in all the three enriched terms in HAM/TSP vs. ATLL. The constructed interaction network was then visualized inside a mirrored map of signaling pathways for ATLL and HAM/TSP, so that the functions of hub genes were specified in both diseases.
Authentication of Aspergillus parasiticus strains in the genome database of the National Center for Biotechnology Information
Authors: Chang Perng-Kuang
Published: March 2021

Objective

The use of genome sequences from strains authenticated to correct species level is a prerequisite for confidently exploring the evolutionary relationship among related species. Aspergillus strains erroneously curated as Aspergillus oryzae and Aspergillus fumigatus have been noticed in the National Center for Biotechnology Information (NCBI) genome database. Aspergillus parasiticus is one of several aspergilli that produce aflatoxin, the most potent carcinogenic mycotoxin known up to now. To ensure that valid conclusions are drawn by researchers from their genomics-related studies, molecular analyses were carried out to authenticate identities of A. parasiticus strains in the NCBI genome database.

Results

Two of the nine supposedly A. parasiticus strains, E1365 and NRRL2999, were found to be misidentified. They turned out to be Aspergillus flavus based on genome-wide single nucleotide polymorphisms (SNPs) and genetic features associated with production of aflatoxin and cyclopiazonic acid. NRRL2999 lacked the additional partial aflatoxin gene cluster known to be present in its equivalent strain, designated as SU-1, and shared a very low total SNPs count specifically with A. flavus NRRL3357 but not with other A. flavus isolates. Therefore, the mislabeled NRRL2999 strain actually is a clonal strain of A. flavus NRRL3357, whose genome was first sequenced in 2005.
Carotid plaque inflammatory activity assessed by 2-[18F]FDG-PET imaging decrease after a neurological thromboembolic event
Authors: Urbak Laerke , Ripa Rasmus S. , Sandholt Benjamin V. , Kjaer Andreas , Sillesen Henrik , Graebe Martin
Published: March 2021

Background

Atherosclerotic plaque vulnerability is comprised by plaque composition driven by inflammatory activity and these features can be depicted with 3D ultrasound and 2-[18F]FDG-PET, respectively. The study investigated timely changes in carotid artery plaque inflammation and morphology after a thromboembolic event with PET/CT and novel ultrasound volumetric grayscale median (GSM) readings. Patients with a single hemisphere-specific neurological symptom and the presence of an ipsilateral carotid artery atherosclerotic plaque were prospectively included to both 2-[18F]FDG PET/CT and 3D ultrasound scans of the plaque immediately after their event and again three months later. On PET/CT images the maximum standardized uptake value (SUVmax) was measured and the volumetric ultrasound acquisitions were analyzed using a semiautomated software measuring GSM values.

Results

Baseline scans were performed by a mean of 7 days (range 2–14) after the symptom and again after 98 days (range 91–176). For the entire group (n = 14), we found a decrease in average SUVmax from baseline to follow-up of − 0.18 (95% confidence interval: − 0.34 to − 0.02, P = 0.034). GSM did not increase significantly over time (mean change: + 2.21, 95% confidence interval: − 17.02 to 21.44, P = 0.808).

Conclusion

A decrease in culprit lesion 2-[18F]FDG-uptake 3 months after an event indicates a decrease in inflammatory activity, suggesting that carotid plaque stabilization over time. 3D ultrasound morphological quantitative differences in GSM were not detectable after 3 months.
Serum soluble CD26/DPP4 titer variation is a potential prognostic biomarker in cancer therapy with a humanized anti-CD26 antibody
Authors: Kaneko Yutaro , Hatano Ryo , Hirota Naoto , Isambert Nicolas , Trillet-Lenoir Véronique , You Benoit , Alexandre Jérôme , Zalcman Gérard , Valleix Fanny , Podoll Thomas , Umezawa Yoshimi , Takao Seiichi , Iwata Satoshi , Hosono Osamu , Taguchi Tetsuo , Yamada Taketo , Dang Nam H. , Ohnuma Kei , Angevin Eric , Morimoto Chikao
Published: March 2021

Background

The phase I trial of the humanized anti-CD26 monoclonal antibody YS110 for CD26-expressing tumors was conducted recently. The present study identifies a potential prognostic biomarker for CD26-targeted therapy based on the phase I data.

Methods

Box and Whisker plot analysis, Scatter plot analysis, Peason product moment correlation/Spearman’s rank-difference correlation, Bar graph analysis, and Receiver Operating Characteristics (ROC) were used to examine the correlation between sCD26 titer variation with YS110 administration and tumor volume change, RECIST criteria evaluation and progression free survival (PFS). Mechanism for serum sCD26 titer variation was confirmed by in vitro experimentation.

Results

Serum sCD26/DPP4 titer was reduced following YS110 administration and gradually recovered until the next infusion. Serum sCD26/DPP4 titer before the next infusion was sustained at lower levels in Stable Disease (SD) cases compared to Progressive Disease cases. ROC analysis defined the cut-off level of serum sCD26/DPP4 titer variation at day 29 pre/post for the clinical outcome of SD as tumor response or PFS. In vitro experimentation confirmed that YS110 addition reduced sCD26 production from CD26-expressing tumor and non-tumor cells.

Conclusions

Our study indicates that serum sCD26/DPP4 titer variation in the early phase of YS110 treatment is a predictive biomarker for evaluating therapeutic efficacy.
NGSS-based teacher professional development to implement engineering practices in STEM instruction
Authors: Christian Kimberly B. , Kelly Angela M. , Bugallo Mónica F.
Published: March 2021

Background

With widespread adoption of the Next Generation Science Standards (NGSS) in the USA (US), research is needed on how secondary science, technology, engineering, and mathematics (STEM) teachers conceptualize the integration of engineering knowledge and practices in traditional STEM classrooms. The present study explored the affective impacts of participation in an engineering education workshop for secondary STEM teachers as part of a 200-h professional development program. The workshop focused on the implementation of electrical engineering and biotechnology principles and design practices in disciplinary instruction, as well as training teachers to differentiate among engineering fields and advise on career pathways. The conceptual framework for the workshop design was based upon elements of the interconnected model of professional growth to identify influences contributing to engineering pedagogical self-efficacy and career awareness.

Results

The overarching research questions addressed how professional development in engineering education affected secondary STEM teachers’ beliefs about the value of using engineering design to support learning, their self-efficacy regarding teaching engineering in their courses, perceived obstacles to effective STEM integration, and their confidence advising students about engineering post-secondary study and careers. The convergent parallel mixed methods design involved factor analysis, comparisons of means, and phenomenology with elements of grounded theory. The survey sample included 60 STEM teachers in the treatment group and 28 teachers in the control group. Six science teachers participated in interviews before and after the engineering workshops. Findings indicated that participating teachers significantly improved their confidence in engineering pedagogy, as well as their knowledge of engineering careers and precollege preparation for post-secondary engineering. Teachers expressed their views of engineering as a potentially powerful tool in developing students’ critical thinking and problem-solving skills, particularly when integrating the practices of science and engineering with the instruction of disciplinary content.

Conclusions

The results from this study demonstrate that a university-based professional development workshop series, developed by engineering and science education faculty, is an effective first-step intervention to improve the engineering knowledge and skills of secondary STEM educators, ultimately facilitating NGSS adoption in classroom instruction. Educating teachers on engineering career pathways is another innovation for the promotion of more diverse participation in engineering fields.
Multiple sclerosis patients and COVID-19
Authors: Mado Hubert , Adamczyk-Sowa Monika
Published: March 2021

Coronavirus disease 2019 (COVID-19) is now a major issue for all fields of medicine. Due to the higher mortality rate among patients with chronic diseases, it has also caused concern in patients with multiple sclerosis (MS), who in addition are often receiving immunosuppressive drugs. The aim of this article is to discuss what is currently known about the severity of COVID-19 in MS patients.
Vulvar lipoma: So giant and rare?
Authors: Büyüközsoy Ayşegül Karadayı , Aydıner Ömer
Published: March 2021

Background

Lipomas are often small tumors and rarely reach a size larger than 2 cm. Vulvar lipoma is a rare tumor localization, and only a few cases have been reported. It is important to distinguish lipomas from other vulvar masses like liposarcomas, Nuck canal cyst, Bartholin’s cyst, and inguinal hernias.

Case presentation

We report on a case of a 43-year-old woman who presented with a giant left vulvar mass and discuss the magnetic resonance imaging (MRI) results.

Conclusions

MRI is useful in evaluating vulvar lipomas and differentiating them from other vulvar pathologies.
Transforming MRCPsych theory examinations: digitisation and very short answer questions (VSAQs)
Authors: Scheeres Karl , Agrawal Niruj , Ewen Stephanie , Hall Ian
Published: March 2021

Many examinations are now delivered online using digital formats, the migration to which has been accelerated by the COVID-19 pandemic. The MRCPsych theory examinations have been delivered in this way since Autumn 2020. The multiple choice question formats currently in use are highly reliable, but other formats enabled by the digital platform, such as very short answer questions (VSAQs), may promote deeper learning. Trainees often ask for a focus on core knowledge, and the absence of cueing with VSAQs could help achieve this. This paper describes the background and evidence base for VSAQs, and how they might be introduced. Any new question formats would be thoroughly piloted before appearing in the examinations and are likely to have a phased introduction alongside existing formats.
The effect of anaesthetic dose on response and remission in electroconvulsive therapy for major depressive disorder: nationwide register-based cohort study
Authors: Kronsell Alexander , Nordenskjöld Axel , Bell Max , Amin Ridwanul , Mittendorfer-Rutz Ellenor , Tiger Mikael
Published: March 2021

Background

Electroconvulsive therapy (ECT) is a safe and effective treatment for major depressive disorder (MDD). ECT treatment effect relies on induced generalised seizures. Most anaesthetics raise the seizure threshold and shorten seizure duration. There are no conclusive studies on the effect of anaesthetic dose on response and remission rates with ECT for MDD.

Aims

We aimed to examine the effect of different dose intervals of anaesthetics on response and remission after ECT for MDD.

Method

We conducted a nationwide cohort study, using data from Swedish registers. Low-, medium- and high-dose intervals, adjusted for age and gender, were constructed for each anaesthetic drug. Response and remission were measured with the Clinical Global Impression – Severity and Improvement scales (CGI-I and CGI-S), and a self-rated version of the Montgomery–Åsberg Depression Rating Scale (MADRS-S). Logistic regression models were used to calculate adjusted odds ratios for response and remission rates.

Results

The study included 7917 patients who received ECT for MDD during 2012–2018. Patients were given either thiopental (64.1%) or propofol (35.9%). Low-dose intervals of anaesthetics were associated with increased rates of response (CGI-I: odds ratio 1.22, 95% CI 1.07–1.40, P = 0.004; MADRS-S: odds ratio 1.31, 95% CI 1.09–1.56, P = 0.004) and remission (CGI-S: odds ratio 1.37, 95% CI 1.17–1.60, P ≤ 0.001; MADRS-S: odds ratio 1.31, 95% CI 1.10–1.54, P = 0.002).

Conclusions

We found improved treatment outcomes with low- compared with high-dose anaesthetic during ECT for MDD. To enhance treatment effect, deep anaesthesia during ECT for MDD should be avoided.
Survival and New Prognosticators in Metastatic Seminoma: Results From the IGCCCG-Update Consortium
Authors: Beyer Jörg , Collette Laurence , Sauvé Nicolas , Daugaard Gedske , Feldman Darren R. , Tandstad Torgrim , Tryakin Alexey , Stahl Olof , Gonzalez-Billalabeitia Enrique , De Giorgi Ugo , Culine Stéphane , de Wit Ronald , Hansen Aaron R. , Bebek Marko , Terbuch Angelika , Albany Costantine , Hentrich Marcus , Gietema Jourik A. , Negaard Helene , Huddart Robert A. , Lorch Anja , Cafferty Fay H. , Heng Daniel Y. C. , Sweeney Christopher J. , Winquist Eric , Chovanec Michal , Fankhauser Christian , Stark Daniel , Grimison Peter , Necchi Andrea , Tran Ben , Heidenreich Axel , Shamash Jonathan , Sternberg Cora N. , Vaughn David J. , Duran Ignacio , Bokemeyer Carsten , Patrikidou Anna , Cathomas Richard , Assele Samson , Gillessen Silke ,   for the International Germ Cell Cancer Classification Update Consortium
Published: March 2021

PURPOSE

The classification of the International Germ-Cell Cancer Collaborative Group (IGCCCG) has been a major advance in the management of germ-cell tumors, but relies on data of only 660 patients with seminoma treated between 1975 and 1990. We re-evaluated this classification in a database from a large international consortium.

MATERIALS AND METHODS

Data on 2,451 men with metastatic seminoma treated with cisplatin- and etoposide-based first-line chemotherapy between 1990 and 2013 were collected from 30 institutions or collaborative groups in Australia, Europe, and North America. Clinical trial and registry data were included. Primary end points were progression-free survival (PFS) and overall survival (OS) calculated from day 1 of treatment. Variables at initial presentation were evaluated for their prognostic impact. Results were validated in an independent validation set of 764 additional patients.

RESULTS

Compared with the initial IGCCCG classification, in our modern series, 5-year PFS improved from 82% to 89% (95% CI, 87 to 90) and 5-year OS from 86% to 95% (95% CI, 94 to 96) in good prognosis, and from 67% to 79% (95% CI, 70 to 85) and 72% to 88% (95% CI, 80 to 93) in intermediate prognosis patients. Lactate dehydrogenase (LDH) proved to be an additional adverse prognostic factor. Good prognosis patients with LDH above 2.5× upper limit of normal had a 3-year PFS of 80% (95% CI, 75 to 84) and a 3-year OS of 92% (95% CI, 88 to 95) versus 92% (95% CI, 90 to 94) and 97% (95% CI, 96 to 98) in the group with lower LDH.

CONCLUSION

PFS and OS in metastatic seminoma significantly improved in our modern series compared with the original data. The original IGCCCG classification retains its relevance, but can be further refined by adding LDH at a cutoff of 2.5× upper limit of normal as an additional adverse prognostic factor.
Multiparty quantum communication protocol based on quantum stabilizer codes with identity authentication
Authors: Li J. X. , He Z. X. , Zhao S. , Yan D. D. , Ma H. Y.
Published: March 2021

Quantum communication has been extensively studied and continuously developed due to its theoretical absolute security. In this work, a novel multiparty quantum communication protocol based on quantum stabilizer codes (QSC) is proposed. Firstly, a trusted third party provides quantum identity authentication (QIA) for users. Secondly, users prepare quantum multiparticle entangled states for transmitting secret information. Thirdly, the secret information is encoded by the QSC to resist channel noise. A part of the encoded qubits is selected to detect the security of the communication. In this protocol, QIA prevents active attacks such as impersonation attacks; the physical laws of quantum entangled state ensure information security; the theory of QSC corrects the flipping errors of qubits, and improves the communication efficiency. In addition, we prove the security of the protocol, and further verify the communication efficiency. Compared with existing protocols, this protocol has better performance on communication efficiency.
19-hydroxy Steroids in the Aromatase Reaction: Review on Expression and Potential Functions
Authors: Abaffy Tatjana , Matsunami Hiroaki
Published: March 2021

Scientific evidence related to the aromatase reaction in various biological processes spanning from mid-1960 to today is abundant; however, as our analytical sensitivity increases, a new look at the old chemical reaction is necessary. Here, we review an irreversible aromatase reaction from the substrate androstenedione. It proceeds in 3 consecutive steps. In the first 2 steps, 19-hydroxy steroids are produced. In the third step, estrone is produced. They can dissociate from the enzyme complex and either accumulate in tissues or enter the blood.

In this review, we want to highlight the potential importance of these 19-hydroxy steroids in various physiological and pathological conditions. We focus primarily on 19-hydroxy steroids, and in particular on the 19-hydroxyandrostenedione produced by the incomplete aromatase reaction. Using a PubMed database and the search term “aromatase reaction,” 19-hydroxylation of androgens and steroid measurements, we detail the chemistry of the aromatase reaction and list previous and current methods used to measure 19-hydroxy steroids.

We present evidence of the existence of 19-hydroxy steroids in brain tissue, ovaries, testes, adrenal glands, prostate cancer, as well as during pregnancy and parturition and in Cushing’s disease. Based on the available literature, a potential involvement of 19-hydroxy steroids in the brain differentiation process, sperm motility, ovarian function, and hypertension is suggested and warrants future research.

We hope that with the advancement of highly specific and sensitive analytical methods, future research into 19-hydroxy steroids will be encouraged, as much remains to be learned and discovered.
Tyrosine sulfation and O-glycosylation of chemoattractant receptor GPR15 differentially regulate interaction with GPR15L
Authors: Okamoto Yukari , Shikano Sojin
Published: March 2021

GPR15 is a G-protein-coupled receptor (GPCR) that directs lymphocyte homing to the colon and skin. Recent studies have identified a chemokine-like protein GPR15L (also known as C10orf99) as a functional ligand of GPR15. In this study, we examined the structural elements that regulate the GPR15–GPR15L interaction with primary focus on post-translational modifications (PTMs) of receptor N-terminus and on the C-terminus of the ligand. Our findings reveal that the GPR15 receptor is sulfated on the N-terminal tyrosine residue(s) and disruption of tyrosine sulfation inhibits binding of GPR15L. In contrast, the disruption of O-glycosylation on the N-terminal threonine or serine residues, or the removal of α2,3-linked sialic acids from O-glycans, enhances the GPR15L binding. Thus, GPR15 represents a unique chemoattractant receptor in which different N-terminal PTMs regulate its ligand binding in a contrasting manner. We further demonstrate that, unlike canonical chemokines, GPR15L activity critically requires its extreme C-terminal residue and that its hydrophobicity may be a key attribute that facilitates an optimal interaction with the receptor. Our results reveal novel insights into chemoattractant receptor–ligand interaction and provide a valid footing for potential intervention targeting the GPR15–GPR15L axis.
AKR1B10 negatively regulates autophagy through reducing GAPDH upon glucose starvation in colon cancer
Authors: Li Wanyun , Liu Cong , Huang Zilan , Shi Lei , Zhong Chuanqi , Zhou Wenwen , Meng Peipei , Li Zhenyu , Wang Shengyu , Luo Fanghong , Yan Jianghua , Wu Ting
Published: March 2021

Autophagy is considered to be an important switch for facilitating normal to malignant cell transformation during colorectal cancer development. Consistent with other reports, we found that the membrane receptor Neuropilin1 (NRP1) is greatly upregulated in colon cancer cells that underwent autophagy upon glucose deprivation. However, the mechanism underlying NRP1 regulation of autophagy is unknown. We found that knockdown of NRP1 inhibits autophagy and largely upregulates the expression of aldo-keto reductase family 1 B10 (AKR1B10). Moreover, we demonstrated that AKR1B10 interacts with and inhibits the nuclear importation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and then subsequently represses autophagy. Interestingly, we also found that an NADPH-dependent reduction reaction could be induced when AKR1B10 interacts with GAPDH, and the reductase activity of AKR1B10 is important for its repression of autophagy. Together, our findings unravel a novel mechanism of NRP1 in regulating autophagy through AKR1B10.
Η μάταιη θεραπεία (futile treatment): Ιατρική και θεολογική προσέγγιση
Authors: Volteas Spiridon K , Fanaras Vasileios
Published: March 2021

This is a medical and theological approach of Futile Treatment, an issue that raises severe medical and bioethical dilemmas. We discuss the difficulties in establishing objective criteria to discontinue treatment and present the theological views on the subject with emphasis to orthodox theology. This paper aims to contribute to the subject and also rise a debate in Greece.
Lie detection in the brain - Moral, social, legal and religious challenges
Authors: Poulou Chrysa G
Published: March 2021

Lie detection and recognition has been an ardent human desire since ancient times. Over the centuries, various methods have been devised to detect fraud. Some methods are low-tech, like the recognition of specialized facial expressions, while others use devices, such as the polygraph or "lie detector" designed in the early 20th century, which measure changes in human body like sweating, heart rate and blood pressure, in order to detect the lie. These methods have various applications in areas such as justice, the military and the secret services. Recent attempts to detect falsehood have focused on measuring brain activity. This approach, unlike previous emotional arousal methods, detects physiological changes associated with cognitive processes during deception and therefore, according to the proponents of the technique, could detect the process of deception itself. The most well-known method of detecting a "neurophysiological difference between deception and truth" in the brain is functional Magnetic Resonance Imaging (fMRI), an MRI brain scan which reveals that deception is located in the prefrontal cortex, just behind the forehead.However, how accurate and reliable are these “brain mapping” approaches to detect lies? What are the moral, legal, religious and social issues arising from the “invasion” of so-called neuroscience into personal data, individual freedom, and the right of non-self-incrimination and the "free will" of human beings? Critical questions, addressed in this paper, in an attempt to approach lie detection in the brain and the challenges - concerns that may determine the way we perceive human society in the future.
Sustainable Biofuel Policies: Challenges and ethical considerations
Authors: Tsitrouli Zinovia
Published: March 2021

Over the last few decades, after the emergence of biofuels at commercial scale in the 1970s, several policies, at domestic or international level, have promoted these alternative fuels, citing mainly three supporting reasons: their potential to fight energy insecurity, ability to alleviate climate change through decreasing carbon dioxide in the atmosphere -compared to conventional fuels- and capacity to promote agriculture and rural development. But, ever since the beginning of their expansion, it became clear that biofuels are not as sustainable as originally thought. And since policies and national legislative frameworks are the main instruments guiding biofuels’ development, this article aims to focus on answering the question whether current biofuel policies are efficient in ensuring sustainability, while promoting biofuels. If this is found not to be the case, then an attempt will be made in order to examine the relative existing gaps, to discuss the potential conflicting interests and ultimately to proceed with recommending adequate alterations in legislations. Ethical dimensions will also be discussed, which could provide some insightful considerations for upcoming biofuel legislations.
Population attributable risk for colorectal and breast cancer in England, Wales, Scotland, Northern Ireland, and the United Kingdom
Authors: Goon Shatabdi , Kim Hanseul , Giovannucci Edward L.
Published: March 2021

Background: The population attributable risk (PAR) is a statistic commonly used for quantifying preventability of cancer. We report here PAR estimates for the United Kingdom (UK) along with its constituent countries for up-to-date risk factor-attributable colorectal cancer (CRC) and breast cancer (BC), focusing on diet and nutrition related factors and tobacco (CRC) using representative national surveys.

Methods: The PAR was calculated using established, modifiable risk factors by the World Cancer Research Fund/American Institute of Cancer Research (WCRF/AICR): physical activity, body mass index (BMI), alcoholic drinks, red meat, processed meat, dietary fiber, dietary calcium, as well as cigarette smoking for CRC, and physical activity, BMI, alcoholic drinks, and fruits and vegetable consumption for BC. National prevalence estimates and relative risks (RRs) for CRC and BC were obtained from meta-analyses or large pooled analyses.

Results: Based on eight dietary and lifestyle risk factors, the estimates for attributable cases of CRC for males and females, respectively, were as follows: England: 67% and 60%; Scotland: 68% and 59%, Wales: 66% and 61%; Northern Ireland: 67% and 61%; and UK: 67% and 60%. Excluding smoking, the PAR for the UK was 61% for men and 52% for women. Based on four dietary and lifestyle risk factors, the estimates for BC were as follows: England: 26%, Scotland: 27%; Wales: 25%; Northern Ireland: 26%; and UK: 27%.

Conclusion: Up to 67% for CRC and 27% of BC were attributable to modifiable dietary and lifestyle factors in the UK. Moderate differences in PAR are observed between countries due to different prevalence of exposure to risk factors.
All thresholds of maternal hyperglycaemia from the WHO 2013 criteria for gestational diabetes identify women with a higher genetic risk for type 2 diabetes
Authors: Hughes Alice E. , Hayes M. Geoffrey , Egan Aoife M. , Patel Kashyap A. , Scholtens Denise M. , Lowe Lynn P. , Lowe Jr William L. , Dunne Fidelma P. , Hattersley Andrew T. , Freathy Rachel M.
Published: March 2021

Background: Using genetic scores for fasting plasma glucose (FPG GS) and type 2 diabetes (T2D GS), we investigated whether the fasting, 1-hour and 2-hour glucose thresholds from the WHO 2013 criteria for gestational diabetes (GDM) have different implications for genetic susceptibility to raised fasting glucose and type 2 diabetes in women from the Hyperglycemia and Adverse Pregnancy Outcome (HAPO) and Atlantic Diabetes in Pregnancy (DIP) studies.

Methods: Cases were divided into three subgroups: (i) FPG ≥5.1 mmol/L only, n=222; (ii) 1-hour glucose post 75 g oral glucose load ≥10 mmol/L only, n=154 (iii) 2-hour glucose ≥8.5 mmol/L only, n=73; and (iv) both FPG ≥5.1 mmol/L and either of a 1-hour glucose ≥10 mmol/L or 2-hour glucose ≥8.5 mmol/L, n=172. We compared the FPG and T2D GS of these groups with controls (n=3,091) in HAPO and DIP separately.

Results: In HAPO and DIP, the mean FPG GS in women with a FPG ≥5.1 mmol/L, either on its own or with 1-hour glucose ≥10 mmol/L or 2-hour glucose ≥8.5 mmol/L, was higher than controls (all P <0.01). Mean T2D GS in women with a raised FPG alone or with either a raised 1-hour or 2-hour glucose was higher than controls (all P <0.05). GDM defined by 1-hour or 2-hour hyperglycaemia only was also associated with a higher T2D GS than controls (all P <0.05).

Conclusions: The different diagnostic categories that are part of the WHO 2013 criteria for GDM identify women with a genetic predisposition to type 2 diabetes as well as a risk for adverse pregnancy outcomes.
Perspectives of different stakeholders on data use and management in public health emergencies in sub-Saharan Africa: a meeting report
Authors: Munung Nchangwi  Syntia , Chi Primus Che , Abayomi Akin , Afolabi Muhammed O. , Ambe Jennyfer , Bonarwolo Korlia , Yusuf Kabba , Kajoleh Francis Kombe , Staunton Ciara , Ujewe Samuel , Tangwa Godfrey B.
Published: March 2021

During public health emergencies (PHEs), data are collected and generated from a variety of activities and sources, including but not limited to national public health programs, research and community-based activities. It is critical that these data are rapidly shared in order to facilitate the public health response, epidemic preparedness, as well as research during and after the epidemic. Nonetheless, collecting and sharing data during PHEs can be challenging, especially where there are limited resources for public health and research-related activities. In a symposium that brought together different stakeholders that were involved in the 2013-2016 Ebola outbreaks in West Africa, meeting attendees shared their perspectives on the values and management of data during PHEs in sub-Saharan Africa. Key factors that could inform and facilitate data management during PHEs in sub-Saharan Africa were discussed, including using data to inform policy decisions and healthcare; a coordinated data collection and management scheme; identifying incentives for data sharing; and equitable data  governance mechanism that emphasise principles of reciprocity, transparency and accountability rather that trust between stakeholders or collaborators. Empirical studies are required to explore how these principles could inform best practices for data management and governance during PHE in sub-Saharan Africa.
The diversity, abundance and seasonal distribution of planktonic microcrustacean (Copepoda, Cladocera) in Kayalıköy Reservoir (Kırklareli/Turkey)
Authors: Güher Hüseyin , Öterler Burak
Published: March 2021

This study was carried out to determine the diversity, abundance and seasonal distribution of planktonic microcrustaceans (Cladocera, Copepoda) in Kayalıköy Reservoir. Cladocera and Copepoda samples were collected from May 2018 to April 2019 at three stations in the reservoir and some environmental parameters were measured. The qualitative evaluation of the samples revealed presence of 40 species, 24 from Cladocera and 16 from Copepoda, in the reservoir. The quantitative evaluation of the samples showed that 52690 ind./m³ zooplankton on average was found in the reservoir. The most common species in the reservoir were Daphnia cucullata, D. longispina, D. pulex, Bosmina longirostris and Chydorus sphaericus from Cladocera, and Arctodiaptomus wierzejskii, Acanthocyclops robustus and Thermocyclops crassus from Copepoda. According to the water quality standards, the measured values of water temperature, dissolved oxygen, sulfate, salinity and chloride indicated the first quality of water, while the light permeability, nitrite-nitrogen, nitrate-nitrogen, ortho-phosphate and chlorophyll-a values indicated the second and third quality of water levels. According to these results, we conclude that Kayalıköy Reservoir has a mesotrophic character in terms of the microcrustacean fauna and the physicochemical parameters.