bluuebunny/crossref_metadata_2025 huggingface dataset:
How Major Central Banks Reacted to the Inflationary Wave of the Early 2020s : The Case of the ECB
Authors: Durkó Ruben
Published: December 2023

From 2021 onwards, inflationary pressures around the world have forced most central banks to raise interest rates from a level that had long been around 0 per cent. However, some central banks, including the European Central Bank (ECB), have often been the subject of criticism for not paying enough attention to the emerging inflationary spiral. The author examines whether there is evidence that the ECB started to tighten monetary conditions later than it had done in the past. The interest rate rule widely applied by the academic community and the results of the pseudo-forecast based on this rule show that the ECB moved later than its historical behaviour would suggest, i.e. it started tightening three quarters later than indicated in the model; nevertheless, by the end of 2022, the actual and the theoretical rates were in line thanks to the intensive rate hikes.
Evolutionary Analysis of a Parrot Bornavirus 2 Detected in a Sulphur-Crested Cockatoo (Cacatua galerita) Suggests a South American Ancestor
Authors: Chacón Ruy D. , Sánchez-Llatas Christian J. , Diaz Forero Andrea J. , Guimarães Marta B. , Pajuelo Sarah L. , Astolfi-Ferreira Claudete S. , Ferreira Antonio J. Piantino
Published: December 2023

Parrot bornavirus (PaBV) is an RNA virus that causes Proventricular Dilatation Disease (PDD), neurological disorders, and death in Psittaciformes. Its diversity in South America is poorly known. We examined a Cacatua galerita presenting neuropathies, PDD, and oculopathies as the main signs. We detected PaBV through reverse transcription polymerase chain reaction (RT-PCR) and partial sequencing of the nucleoprotein (N) and matrix (M) genes. Maximum likelihood and Bayesian phylogenetic inferences classified it as PaBV-2. The nucleotide identity of the sequenced strain ranged from 88.3% to 90.3% against genotype PaBV-2 and from 80.2% to 84.4% against other genotypes. Selective pressure analysis detected signs of episodic diversifying selection in both the N and M genes. No recombination events were detected. Phylodynamic analysis estimated the time to the most recent common ancestor (TMRCA) as the year 1758 for genotype PaBV-2 and the year 1049 for the Orthobornavirus alphapsittaciforme species. Substitution rates were estimated at 2.73 × 10−4 and 4.08 × 10−4 substitutions per year per site for N and M, respectively. The analysis of population dynamics showed a progressive decline in the effective population size during the last century. Timescale phylogeographic analysis revealed a potential South American ancestor as the origin of genotypes 1, 2, and 8. These results contribute to our knowledge of the evolutionary origin, diversity, and dynamics of PaBVs in South America and the world. Additionally, it highlights the importance of further studies in captive Psittaciformes and the potential impact on endangered wild birds.
Application of Near-Infrared Reflectance Spectroscopy for Predicting Chemical Composition of Feces in Holstein Dairy Cows and Calves
Authors: Xu Yiming , Chen Tianyu , Zhang Hongxing , Nuermaimaiti Yiliyaer , Zhang Siyuan , Wang Fei , Xiao Jianxin , Liu Shuai , Shao Wei , Cao Zhijun , Wang Jingjun , Chen Yong
Published: December 2023

Traditional methods for determining the chemical composition of cattle feces are uneconomical. In contrast, near-infrared reflectance spectroscopy (NIRS) has emerged as a successful technique for assessing chemical compositions. Therefore, in this study, the feasibility of NIRS in terms of predicting fecal chemical composition was explored. Cattle fecal samples were subjected to chemical analysis using conventional wet chemistry techniques and a NIRS spectrometer. The resulting fecal spectra were used to construct predictive equations to estimate the chemical composition of the feces in both cows and calves. The coefficients of determination for calibration (RSQ) were employed to evaluate the calibration of the predictive equations. Calibration results for cows (dry matter [DM], RSQ = 0.98; crude protein [CP], RSQ = 0.93; ether extract [EE], RSQ = 0.91; neutral detergent fiber [NDF], RSQ = 0.82; acid detergent fiber [ADF], RSQ = 0.89; ash, RSQ = 0.84) and calves (DM, RSQ = 0.92; CP, RSQ = 0.89; EE, RSQ = 0.77; NDF, RSQ = 0.76; ADF, RSQ = 0.92; ash, RSQ = 0.97) demonstrated that NIRS is a cost-effective and efficient alternative for assessing the chemical composition of dairy cattle feces. This provides a new method for rapidly predicting fecal chemical content in cows and calves.
Effects of Climatic Conditions and Supplementation with Palm Cake on the Thermoregulation of Crossbred Buffaloes Raised in a Rotational Grazing System and with Natural Shade in Humid Tropical Regions
Authors: Brcko Carolina Carvalho , Silva Jamile Andrea Rodrigues da , Garcia Alexandre Rossetto , Silva André Guimarães Maciel e , Martorano Lucieta Guerreiro , Vilela Reíssa Alves , Nahúm Benjamim de Souza , Barbosa Antônio Vinícius Corrêa , Silva Welligton Conceição da , Rodrigues Thomaz Cyro Guimarães de Carvalho , Silva Éder Bruno Rebelo da , Lourenço-Júnior José de Brito
Published: December 2023

In ruminants, diet composition has a positive correlation with heat production, which can influence thermoregulation, energy expenditure and, consequently, animal performance. The objective of this work was to evaluate the effects of climatic conditions and supplementation based on palm kernel cake, on the thermoregulation of crossbred buffaloes in the eastern Amazon. The research was carried out at Embrapa Amazônia Oriental (01°26′ S and 48°24′ W), Belém, Pará, and lasted 12 months (representing the entire year). Twenty-four buffaloes, females, with initial age and an average weight of 54 ± 7 months and 503.1 ± 23 kg, respectively, non-pregnant, non-lactating and clinically healthy were used, divided into four treatments based on the supplementation content of the palm cake (%DM) in relation to their body weight (%): 0, 0.25, 0.50 and 1.0. The animals were kept in paddocks with Brachiaria brizantha (cv. Marandu), in a rotating system, with water to drink and mineral salt ad libitum. Equipment was installed to record environmental data (temperature and relative humidity, dew point temperature, wet bulb and black globe) and physiological data: rectal temperature (RT); respiratory rate (RR); and body surface temperature (BST), recorded twice a day, always in the morning (6:00 a.m. to 7:00 a.m.) and afternoon (12:00 p.m. to 1:00 p.m.) shifts, and were used to calculate the Globe Temperature and Humidity Index (GTHI). Supplementation did not influence the physiological variables of thermoregulation (p > 0.05). However, there were differences in the GTHI between the shifts, with higher means in the afternoon shift, especially in the less rainy period of the year, where the GTHI reached 92.06 ± 2.74 (p < 0.05). In all periods of the year, the mean values of RT, RR and BST were higher in the afternoon shift (p > 0.05). The respiratory rate (RR) is associated with the annual seasonality of the thermal waters, with higher averages in the afternoons of the rainy season. The positive correlation for rectal temperature, respiratory rate and body surface temperature indicated that buffaloes respond to thermal elevations in the atmosphere (afternoon period) and, consequently, reflect on the GTHI. Supplementation does not influence thermoregulation; the changes observed occurred in response to the region’s thermal and rainfall conditions (mainly in the afternoon shift), with higher GTHI values.
Estimating the Effect of the Kappa Casein Genotype on Milk Coagulation Properties in Israeli Holstein Cows
Authors: Lavon Yaniv , Weller Joel I. , Zeron Yoel , Ezra Ephraim
Published: December 2023

In Israel, about 26% of produced milk is used to produce hard cheeses and 29% for soft cheeses. Milk with preferred coagulation properties requires a shorter coagulation time and yields a higher curd firmness than milk with inferior coagulation properties. Studies have shown that milk from cows with the B allele of kappa casein (κ-CN) produces more cheese than milk from those with A and E alleles. There is evidence that milk from AE or EE genotype cows is unsuitable for cheese production. In the early 1990s, the proportion of the B allele in Israeli Holstein cattle was about 17%, similar to its prevalence in the Holstein population worldwide. In recent years, however, its proportion has increased to about 40%. We analyzed milk coagulation properties as a function of the cow’s κ-CN genotype, including time in minutes until the beginning of coagulation and curd firmness after 60 min—measured in volts via an optigraph device and scored on a scale of 0–4 by a laboratory technician. Cow selection was based on their sire’s genotype, so that there would be sufficient genotypes that include the rare E allele. A total of 359 cows were sampled from 15 farms: 64 with genotype AA, 142 with AB, 41 with AE, 65 with BB, and 47 with BE. Data were analyzed via the general linear model procedure of SAS. We found the following: (a) There were significant differences between genotypes for optigraph-measured curd firmness. In a multi-comparison test, the BB genotype gave the highest curd firmness, and AB and BE showed a significant advantage compared to AA and AE (9.4, 8.6, 8.4, 6.9, 6.8 V, respectively). Assuming a frequency of about 55% for the A allele, about 30% of the milk delivered to dairy plants comes from AA cows. (b) There was a significant difference between the genotypes in technician-observed curd firmness, with BB scoring significantly higher than AA and AE. (c) The optigraph-measured curd firmness was significantly higher for milk from primiparous cows as compared to milk from second, third, or fourth lactation cows (8.9, 7.8, 7.9, 7.7 V, respectively). The technician-observed curd firmness was significantly higher for primiparous vs. multiparous cows. There was a clear advantage in curd firmness for genotypes that included the B allele compared to those with AA and AE genotypes. We can increase the proportion of the B allele in the population by insemination of cows using bulls with the genotypes AB and BB. This factor should therefore be included in the selection index.
A Dual-Tree–Complex Wavelet Transform-Based Infrared and Visible Image Fusion Technique and Its Application in Tunnel Crack Detection
Authors: Wang Feng , Chen Tielin
Published: December 2023

Computer vision methods have been widely used in recent years for the detection of structural cracks. To address the issues of poor image quality and the inadequate performance of semantic segmentation networks under low-light conditions in tunnels, in this paper, infrared images are used, and a preprocessing method based on image fusion technology is developed. First, the DAISY descriptor and the perspective transform are applied for image alignment. Then, the source image is decomposed into high- and low-frequency components of different scales and directions using DT-CWT, and high- and low-frequency subband fusion rules are designed according to the characteristics of infrared and visible images. Finally, a fused image is reconstructed from the processed coefficients, and the fusion results are evaluated using the improved semantic segmentation network. The results show that using the proposed fusion method to preprocess images leads to a low false alarm rate and low missed detection rate in comparison to those using the source image directly or using the classical fusion algorithm.
Spatial Distribution of Ammonia Concentrations and Modeled Dry Deposition in an Intensive Dairy Production Region
Authors: Leytem April B. , Walker John T. , Wu Zhiyong , Nouwakpo Kossi , Baublitz Colleen , Bash Jesse , Beachley Gregory
Published: December 2023

Agriculture generates ~83% of total US ammonia (NH3) emissions, potentially adversely impacting sensitive ecosystems through wet and dry deposition. Regions with intense livestock production, such as the dairy region of south-central Idaho, generate hotspots of NH3 emissions. Our objective was to measure the spatial and temporal variability of NH3 across this region and estimate its dry deposition. Ambient NH3 was measured using diffusive passive samplers at 8 sites in two transects across the region from 2018–2020. NH3 fluxes were estimated using the Surface Tiled Aerosol and Gaseous Exchange (STAGE) model. Peak NH3 concentrations were 4–5 times greater at a high-density dairy site compared to mixed agriculture/dairy or agricultural sites, and 26 times greater than non-agricultural sites with prominent seasonal trends driven by temperature. Annual estimated dry deposition rates in areas of intensive dairy production can approach 45 kg N ha−1 y−1, compared to <1 kg N ha−1 y−1 in natural landscapes. Our results suggest that the natural sagebrush steppe landscapes interspersed within and surrounding agricultural areas in southern Idaho receive NH3 dry deposition rates within and above the range of nitrogen critical loads for North American deserts. Finally, our results highlight a need for improved understanding of the role of soil processes in NH3 dry deposition to arid and sparsely vegetated natural ecosystems across the western US.
Financial Toxicity among Patients with Breast Cancer during the COVID-19 Pandemic in the United States
Authors: Wu Yan , Liu Xianchen , Maculaitis Martine C. , Li Benjamin , Berk Alexandra , Massa Angelina , Weiss Marisa C. , McRoy Lynn
Published: December 2023

This study reported the prevalence of financial distress (financial toxicity (FT)) and COVID-19-related economic stress in patients with breast cancer (BC). Patients with BC were recruited from the Ciitizen platform, Breastcancer.org, and patient advocacy groups between 30 March and 6 July 2021. FT was assessed with the COmprehensive Score for financial Toxicity (COST) instrument. COVID-19-related economic stress was assessed with the COVID-19 Stress Scale. Among the 669 patients, the mean age was 51.6 years; 9.4% reported a COVID-19 diagnosis. The prevalence rates of mild and moderate/severe FT were 36.8% and 22.4%, respectively. FT was more prevalent in patients with metastatic versus early BC (p < 0.001). The factors associated with FT included income ≤ USD 49,999 (adjusted odds ratio (adj OR) 6.271, p < 0.0001) and USD 50,000–USD 149,999 (adj OR 2.722, p < 0.0001); aged <50 years (adj OR 3.061, p = 0.0012) and 50–64 years (adj OR 3.444, p = 0.0002); living alone (adj OR 1.603, p = 0.0476); and greater depression severity (adj OR 1.155, p < 0.0001). Black patients (adj OR 2.165, p = 0.0133), patients with income ≤ USD 49,999 (adj OR 1.921, p = 0.0432), or greater depression severity (adj OR 1.090, p < 0.0001) were more likely to experience COVID-19-related economic stress. FT was common in patients with BC, particularly metastatic disease, during COVID-19. Multiple factors, especially lower income and greater depression severity were associated with financial difficulties during COVID-19.
Photodynamic Therapy and Immunological View in Gastrointestinal Tumors
Authors: Aebisher David , Woźnicki Paweł , Dynarowicz Klaudia , Kawczyk-Krupka Aleksandra , Cieślar Grzegorz , Bartusik-Aebisher Dorota
Published: December 2023

Gastrointestinal cancers are a specific group of oncological diseases in which the location and nature of growth are of key importance for clinical symptoms and prognosis. At the same time, as research shows, they pose a serious threat to a patient’s life, especially at an advanced stage of development. The type of therapy used depends on the anatomical location of the cancer, its type, and the degree of progression. One of the modern forms of therapy used to treat gastrointestinal cancers is PDT, which has been approved for the treatment of esophageal cancer in the United States. Despite the increasingly rapid clinical use of this treatment method, the exact immunological mechanisms it induces in cancer cells has not yet been fully elucidated. This article presents a review of the current understanding of the mode of action of photodynamic therapy on cells of various gastrointestinal cancers with an emphasis on colorectal cancer. The types of cell death induced by PDT include apoptosis, necrosis, and pyroptosis. Anticancer effects are also a result of the destruction of tumor vasculature and activation of the immune system. Many reports exist that concern the mechanism of apoptosis induction, of which the mitochondrial pathway is most often emphasized. Photodynamic therapy may also have a beneficial effect on such aspects of cancer as the ability to develop metastases or contribute to reducing resistance to known pharmacological agents.
Changing Prevalence of AIDS and Non-AIDS-Defining Cancers in an Incident Cohort of People Living with HIV over 28 Years
Authors: Cattelan Anna Maria , Mazzitelli Maria , Presa Nicolò , Cozzolino Claudia , Sasset Lolita , Leoni Davide , Bragato Beatrice , Scaglione Vincenzo , Baldo Vincenzo , Parisi Saverio Giuseppe
Published: December 2023

Background: The introduction and evolution of antiretrovirals has changed the panorama of comorbidities in people living with HIV (PLWH) by reducing the risk of AIDS-defining cancers (ADC). By contrast, due to ageing and persistent inflammation, the prevalence and incidence of non-AIDS-defining cancers have significantly increased. Therefore, we aimed at describing cancer epidemiology in our cohort over 28 years. Methods: We retrospectively included all PLWH in our clinic who ever developed cancers, considering features of ADC and NADC, from January 1996 to March 2023. Demographic, clinical characteristics, and survival were analyzed, comparing three observation periods (1996–2003, 2004–2013, and 2014–2023). Results: A total of 289 PLWH developed 308 cancers over the study period; 77.9% were male, the mean age was 49.6 years (SD 12.2), and 57.4% PLWH developed NADC and 41.5% ADC. Kaposi (21.8%) and non-Hodgkin lymphoma (20.1%) were the most frequent cancers. Age at the time of cancer diagnosis significantly increased over time (41.6 years in the first period vs. 54.4 years in the third period, p < 0.001). In the first period compared with the last, a simultaneous diagnosis of HIV infection and cancer occurred in a higher proportion of persons (42.7 vs. 15.3, p < 0.001). While viro-immunological control at cancer diagnosis significantly improved over time, the proportions of cancer progression/remission remained stable. Overall survival significantly increased, but this trend was not confirmed for ADC. Conclusions: The probability of survival for ADC did not decrease as significantly as the number of ADC diagnoses over time. By contrast, NADC dramatically increased, in line with epidemiological studies and other literature data. The changing patterns of malignancies from ADC to NADC underline the need for public health interventions and the fostering of screening programs aimed at the prevention and early detection of NADC in PLWH.
Efficacy and Safety of Liver Chemoembolization Procedures, Combined with FOLFIRI Chemotherapy, in First-Line Treatment of Metastatic Colorectal Cancer in Patients with Oncogene Mutations
Authors: Szemitko Marcin , Falkowski Aleksander , Modrzejewska Monika , Golubinska-Szemitko Elzbieta
Published: December 2023

Purpose. The usual first- and second-line treatments for inoperable liver metastases from colorectal cancer (CRC) involve systemic chemotherapy, often with molecular targeted therapy. Chemoembolization, using microspheres loaded with irinotecan, has also been available as a treatment option for many years, used mainly in later lines of treatment when, due to increasing resistance, other chemotherapy regimens may have been exhausted. However, when there are contraindications to molecular therapies, the use of chemoembolization as first or second lines of treatment, in combination with FOLFIRI chemotherapy, may provide greater efficacy due to reduced irinotecan resistance. Objective. The aim of the study was to evaluate the efficacy and safety of transarterial chemoembolization (DEB-TACE) procedures for the treatment of metastatic liver lesions from CRC, using irinotecan-loaded microspheres as first-line treatment together with FOLFIRI chemotherapy. Patients and methods. The analysis included 20 patients (12 females; 8 males) with unresectable liver metastases in the course of CRC with KRAS, NRAS and BRAF mutations, who underwent 73 chemoembolization procedures with microspheres loaded with 100 mg of irinotecan, in combination with interspersed FOLFIRI chemotherapy. Response to treatment was assessed through computed tomography according to the Modified Response Evaluation Criteria in Solid Tumors (mRECIST). Progression-free survival (PFS) and overall survival (OS) were calculated using the Kaplan–Meier method. Assessment of adverse events utilized the Cancer Therapy Evaluation Program’s Common Terminology Criteria for Adverse Events (CTCAE; version 5.0). Results. Partial remission (PR) was observed in 11 (55%) patients while 5 (25%) patients showed stable disease (SD). Progression (PD) was observed in 4 (20%) patients. Median PFS was 9.1 months (95% CI: 7.2–10.1 months) and median OS was 20.7 months (95% CI: 18.2–23.3 months). The most common adverse events (AEs) resulting in treatment delay were hematological disorders, notably neutropenia (CTCAE grades 1–3). No deaths or AEs above grade 3 occurred during TACE. Continued FOLFIRI chemotherapy after TACE treatments resulted in grade 4 neutropenia in two patients, grade 3 in four patients and grade 2 thrombocytopenia in two patients. Conclusion. Combining FOLFIRI chemotherapy with chemoembolization procedures for liver metastatic lesions from colorectal cancer may provide a valuable treatment option for patients not qualified for monoclonal antibody therapy.
Analysis of Anxiety, Depression and Fear of Progression at 12 Months Post-Cytoreductive Surgery in the SOCQER-2 (Surgery in Ovarian Cancer—Quality of Life Evaluation Research) Prospective, International, Multicentre Study
Authors: Lakhiani Aarti , Cummins Carole , Kumar Satyam , Long Joanna , Arora Vivek , Balega Janos , Broadhead Tim , Duncan Timothy , Edmondson Richard , Fotopoulou Christina , Glasspool Rosalind , Kolomainen Desiree , Manchanda Ranjit , McNally Orla , Morrison Jo , Mukhopadhyay Asima , Naik Raj , Wood Nick , Sundar Sudha
Published: December 2023

Patients with ovarian cancer (OC) often experience anxiety, depression and fear of progression (FOP); however, it is unclear whether surgical complexity has a role to play. We investigated the prevalence of anxiety, depression and FOP at 12 months post-cytoreductive surgery and investigated associations with surgical complexity, patient (age, ethnicity, performance status, BMI) and tumour (stage, disease load) factors. One hundred and forty-one patients with FIGO Stage III–IV OC, who did not have disease progression at 12 months post-surgery, completed the Hospital Anxiety and Depression Scale and FOP short-form questionnaire. Patients underwent surgery with low (40.4%), intermediate (31.2%) and high (28.4%) surgical complexity scores. At 12 months post-surgery, 99 of 141 (70%) patients with advanced OC undergoing surgery experienced clinically significant anxiety, 21 of 141 (14.9%) patients experienced moderate to severe depression and 37 of 140 (26.4%) experienced dysfunctional FOP. No associations were identified between the three different surgical complexity groups with regards to anxiety, depression or FOP scores. Unsurprisingly, given the natural history of the disease, most patients with OC suffer from anxiety, depression and fear of progression after completion of first-line cancer treatment. Surgical complexity at the time of surgery is not associated with a deleterious impact on anxiety, depression or FOP for patients with OC. Patients with OC experience a profound mental health impact and should be offered mental health support throughout their cancer journey.
Successful Correction by Prime Editing of a Mutation in the RYR1 Gene Responsible for a Myopathy
Authors: Godbout Kelly , Rousseau Joël , Tremblay Jacques P.
Published: December 2023

We report the first correction from prime editing a mutation in the RYR1 gene, paving the way to gene therapies for RYR1-related myopathies. The RYR1 gene codes for a calcium channel named Ryanodine receptor 1, which is expressed in skeletal muscle fibers. The failure of this channel causes muscle weakness in patients, which leads to motor disabilities. Currently, there are no effective treatments for these diseases, which are mainly caused by point mutations. Prime editing allows for the modification of precise nucleotides in the DNA. Our results showed a 59% correction rate of the T4709M mutation in the RYR1 gene in human myoblasts by RNA delivery of the prime editing components. It is to be noted that T4709M is recessive and, thus, persons having a heterozygous mutation are healthy. These results are the first demonstration that correcting mutations in the RYR1 gene is possible.
Adenosine Receptor mRNA Expression in Frontal Cortical Neurons in Schizophrenia
Authors: Sahay Smita , Devine Emily A. , McCullumsmith Robert E. , O’Donovan Sinead M.
Published: December 2023

Schizophrenia is a devastating neuropsychiatric disorder associated with the dysregulation of glutamate and dopamine neurotransmitter systems. The adenosine system is an important neuroregulatory system in the brain that modulates glutamate and dopamine signaling via the ubiquitously expressed adenosine receptors; however, adenosine A1 and A2A receptor (A1R and A2AR) mRNA expression is poorly understood in specific cell subtypes in the frontal cortical brain regions implicated in this disorder. In this study, we assayed A1R and A2AR mRNA expression via qPCR in enriched populations of pyramidal neurons, which were isolated from postmortem anterior cingulate cortex (ACC) tissue from schizophrenia (n = 20) and control (n = 20) subjects using laser microdissection (LMD). A1R expression was significantly increased in female schizophrenia subjects compared to female control subjects (t(13) = −4.008, p = 0.001). A1R expression was also significantly decreased in female control subjects compared to male control subjects, suggesting sex differences in basal A1R expression (t(17) = 2.137, p = 0.047). A significant, positive association was found between dementia severity (clinical dementia rating (CDR) scores) and A2AR mRNA expression (Spearman’s r = 0.424, p = 0.009). A2AR mRNA expression was significantly increased in unmedicated schizophrenia subjects, suggesting that A2AR expression may be normalized by chronic antipsychotic treatment (F(1,14) = 9.259, p = 0.009). Together, these results provide novel insights into the neuronal expression of adenosine receptors in the ACC in schizophrenia and suggest that receptor expression changes may be sex-dependent and associated with cognitive decline in these subjects.
Analysis of Antibacterial and Antiviral Properties of ZnO and Cu Coatings Deposited by Magnetron Sputtering: Evaluation of Cell Viability and ROS Production
Authors: Vibornijs Viktors , Zubkins Martins , Strods Edvards , Rudevica Zhanna , Korotkaja Ksenija , Ogurcovs Andrejs , Kundzins Karlis , Purans Juris , Zajakina Anna
Published: December 2023

The development and testing of antimicrobial coatings continues to be a crucial approach, considering the ongoing emergence of antibiotic-resistant bacteria and the rapid transmission of highly pathogenic viruses. In this study, three types of coatings—pure metallic copper (Cu), zinc oxide (ZnO), and a three-layer zinc oxide and copper mixed coating (ZnO/Cu/ZnO)—were deposited by magnetron sputtering on polyethylene terephthalate substrates to evaluate their antimicrobial potential using various microorganisms, including viruses. Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli bacteria were used for the assessment of antibacterial properties. Antiviral testing was performed using MS2 bacteriophage and replication-deficient Semliki Forest virus, both representing single-stranded RNA-containing viruses. The samples’ ability to cause reactive oxygen species formation was measured, and the effect on bacterial metabolic activity was evaluated. Cu-coated samples showed high inhibitory activity (>95%) against E. coli and S. aureus bacteria, as well as against tested viruses (SFV and MS2). The antibacterial and antiviral properties of ZnO/Cu/ZnO and ZnO coatings were not significant. Although ZnO/Cu/ZnO and ZnO caused inhibition of the metabolic activity of the bacteria, it was insufficient for complete bacteria eradication. Furthermore, significant reactive oxygen species (ROS) production was detected only for single Cu-coated samples, correlating with the strong bacteria-killing ability. We suppose that the ZnO layer exhibited a low release of Zn ions and prevented contact of the Cu layer with bacteria and viruses in the ZnO/Cu/ZnO coating. We conclude that current ZnO and Cu-ZnO-layered coatings do not possess antibacterial and antiviral activity.
Binary Classification with Imbalanced Data
Authors: Chiang Jyun-You , Lio Yuhlong , Hsu Chien-Ya , Ho Chia-Ling , Tsai Tzong-Ru
Published: December 2023

When the binary response variable contains an excess of zero counts, the data are imbalanced. Imbalanced data cause trouble for binary classification. To simplify the numerical computation to obtain the maximum likelihood estimators of the zero-inflated Bernoulli (ZIBer) model parameters with imbalanced data, an expectation-maximization (EM) algorithm is proposed to derive the maximum likelihood estimates of the model parameters. The logistic regression model links the Bernoulli probabilities with the covariates in the ZIBer model, and the prediction performance among the ZIBer model, LightGBM, and artificial neural network (ANN) procedures is compared by Monte Carlo simulation. The results show that no method can dominate the other methods regarding predictive performance under the imbalanced data. The LightGBM and ZIBer models are more competitive than the ANN model for zero-inflated-imbalanced data sets.
Comprehensive Review of Socio-Economic Costs and Benefits, Policy Frameworks, Market Dynamics, and Environmental Implications of Microgrid Development in the UAE
Authors: Sajwani Hussain Abdalla , Soudan Bassel , Olabi Abdul Ghani
Published: December 2023

This research paper presents a comprehensive review of the literature on microgrid development in the UAE, focusing on the socio-economic costs and benefits, policy frameworks, market dynamics, and environmental implications. The analysis encompasses publications from 2011 to 2021, with a particular emphasis on the United Arab Emirates (UAE) and the Gulf Cooperation Council (GCC) countries. A total of 33 papers were identified and classified, revealing gaps in comprehensive valuation models, consideration of environmental and social governance factors, and research informing policy and investment decisions. The findings highlight the significance of microgrid technology in the UAE, its limited adoption and commercialization, and its predominant usage in remote areas and academic testbeds. The paper underscores the UAE’s vision for net-zero emissions by 2050 and the potential of microgrids in supporting its realization. Recommendations include the development of a comprehensive valuation framework to drive effective investments in microgrid technology, aligning with the UAE’s sustainable energy goals. The study contributes to the understanding of microgrid development in the UAE, offering insights into its socio-economic, policy, market, and environmental dimensions.
Power Transformer Fault Detection: A Comparison of Standard Machine Learning and autoML Approaches
Authors: Santamaria-Bonfil Guillermo , Arroyo-Figueroa Gustavo , Zuniga-Garcia Miguel A. , Azcarraga Ramos Carlos Gustavo , Bassam Ali
Published: December 2023

A key component for the performance, availability, and reliability of power grids is the power transformer. Although power transformers are very reliable assets, the early detection of incipient degradation mechanisms is very important to preventing failures that may shorten their residual life. In this work, a comparative analysis of standard machine learning (ML) algorithms (such as single and ensemble classification algorithms) and automatic machine learning (autoML) classifiers is presented for the fault diagnosis of power transformers. The goal of this research is to determine whether fully automated ML approaches are better or worse than traditional ML frameworks that require a human in the loop (such as a data scientist) to identify transformer faults from dissolved gas analysis results. The methodology uses a transformer fault database (TDB) gathered from specialized databases and technical literature. Fault data were processed using the Duval pentagon diagnosis approach and user–expert knowledge. Parameters from both single and ensemble classifiers were optimized through standard machine learning procedures. The results showed that the best-suited algorithm to tackle the problem is a robust, automatic machine learning classifier model, followed by standard algorithms, such as neural networks and stacking ensembles. These results highlight the ability of a robust, automatic machine learning model to handle unbalanced power transformer fault datasets with high accuracy, requiring minimum tuning effort by electrical experts. We also emphasize that identifying the most probable transformer fault condition will reduce the time required to find and solve a fault.
An Improved Hybrid Approach for Daily Electricity Peak Demand Forecasting during Disrupted Situations: A Case Study of COVID-19 Impact in Thailand
Authors: Aswanuwath Lalitpat , Pannakkong Warut , Buddhakulsomsiri Jirachai , Karnjana Jessada , Huynh Van-Nam
Published: December 2023

Accurate electricity demand forecasting is essential for global energy security, reducing costs, ensuring grid stability, and informing decision making in the energy sector. Disruptions often lead to unpredictable demand shifts, posing greater challenges for short-term load forecasting. Understanding electricity demand patterns during a pandemic offers insights into handling future disruptions. This study aims to develop an effective forecasting model for daily electricity peak demand, which is crucial for managing potential disruptions. This paper proposed a hybrid approach to address scenarios involving both government intervention and non-intervention, utilizing integration methods such as stepwise regression, similar day selection-based day type criterion, variational mode decomposition, empirical mode decomposition, fast Fourier transform, and neural networks with grid search optimization for the problem. The electricity peak load data in Thailand during the year of the COVID-19 situation is used as a case study to demonstrate the effectiveness of the approach. To enhance the flexibility and adaptability of the approach, the new criterion of separating datasets and the new criterion of similar day selection are proposed to perform one-day-ahead forecasting with rolling datasets. Computational analysis confirms the method’s effectiveness, adaptability, reduced input, and computational efficiency, rendering it a practical choice for daily electricity peak demand forecasting, especially in disrupted situations.
Numerical Study on the Flow and Heat Transfer Characteristics in Mortise and Tenon Gap under Rotating Conditions
Authors: Xu Yao , Liu Zhao , Sun Rui , Feng Zhenping
Published: December 2023

Mortise and tenon are very important parts of gas turbine dealing operation safety. Additionally, the temperature distribution of the turbine blade and disk is affected by the heat transfer characteristics in its gap. Then, the S-shaped mortise and tenon gap were numerically studied under rotating conditions, and the flow and heat transfer characteristics were analyzed. First, the heat transfer coefficient (HTC) of the mortise and tenon surfaces was measured with thermochromic liquid crystal. Then, the numerical method was verified using the test results, and the grid independence analysis was conducted. Finally, the flow and heat transfer characteristics of the gap under static and rotating conditions were numerically studied, five different Reynolds numbers (Re = 15,000, 20,000, 25,000, 30,000, 35,000) and five gap widths (d = 1 mm, 1.5 mm, 2 mm, 2.5 mm, 3 mm) were conducted and analyzed in detail. The results show that, under the rotating condition, the pressure distribution in the gap is different from that of the static condition; the pressure increases along the radial direction due to the action of centrifugal force and reaches its maximum value at the corner of the “S” shaped structure. With the increase in Re, the heat transfer intensity of the gap increases gradually. Additionally, the heat transfer intensity of the gap increases with an increase in its width.
Auditing and Rating Sustainability of Mediterranean Buildings, Neighbourhoods and Cities
Authors: Balaras Constantinos A. , Droutsa Kalliopi G. , Dascalaki Elena G. , Kontoyiannidis Simon , Moro Andrea , Bazzan Elena , Borgaro Paola
Published: December 2023

Sustainability rating systems for the built environment involve a multitude of indicators that are based on different types of data. This work capitalizes on an existing multicriteria assessment method and supporting decision-making tool at building and neighbourhood scale, to develop an enhanced method and tool at city scale. The main sustainability issues at building and city scales include site and infrastructure, energy and resources consumption, environmental loadings, climate change, environmental quality, water, waste, transportation, services, social aspects, economy and governance. Approximately 300 indicators distributed among the different scales are used to describe and quantify the various facets of sustainability. Specifically, the building scale includes a pool of 80 indicators of which 17 are key performance indicators (KPIs), the neighbourhood scale has 133 indicators of which 14 are KPIs and the city scale has a total of 99 indicators of which 10 are KPIs that were new additions to the existing method. The emphasis in this paper is given on elaborating the key performance indicators for cities and demonstrating their applicability through a case study. The common method and tools provide a flexible assessment system for local authorities and stakeholders to develop and assess sustainability plans.
Rural Depopulation in Greece: Trends, Processes, and Interpretations
Authors: Papadopoulos Apostolos G. , Baltas Pavlos
Published: December 2023

Depopulation is caused by low fertility rates and out-migration, and it applies to countries, regions and smaller areas. Rural depopulation is defined as a sharp population decline that falls well below an adequate population size and indicates that an area has lost its demographic reproductive capacity. This paper discusses the socioeconomic and territorial aspects of rural depopulation, attempting to do justice to the spatial dimensions of the phenomenon. Greece exhibits all the symptoms of demographic transition, leading to labour shortages, declining economic productivity, and increasing demands on the health and welfare system. The study on rural depopulation in Greece focuses on the changes and dynamics observed at the municipal and regional levels. A typology has been developed to identify rural communities in Greece. The main source of demographic data for our study is the Greek censuses (1991, 2001, 2011, and 2021). Demographic and socioeconomic trends in Greece are interlinked and show different regional and local dynamics. Rural depopulation is closely related to the study of (international and internal) migration, even though the latter does not provide a permanent solution to depopulation. An empirical analysis has shown that there is a need to revitalise rural areas through socioeconomic improvements, infrastructure investments, and policies that directly impact rural communities.
Mechanistic Investigation of WWOX Function in NF-kB-Induced Skin Inflammation in Psoriasis
Authors: Shin Min-Jeong , Kim Hyun-Sun , Lee Pyeongan , Yang Na-Gyeong , Kim Jae-Yun , Eun Yun-Su , Lee Whiin , Kim Doyeon , Lee Young , Jung Kyung-Eun , Hong Dongkyun , Shin Jung-Min , Lee Sul-Hee , Lee Sung-Yul , Kim Chang-Deok , Kim Jung-Eun
Published: December 2023

Psoriasis is a chronic inflammatory skin disease characterized by epidermal hyperproliferation, aberrant differentiation of keratinocytes, and dysregulated immune responses. WW domain-containing oxidoreductase (WWOX) is a non-classical tumor suppressor gene that regulates multiple cellular processes, including proliferation, apoptosis, and migration. This study aimed to explore the possible role of WWOX in the pathogenesis of psoriasis. Immunohistochemical analysis showed that the expression of WWOX was increased in epidermal keratinocytes of both human psoriatic lesions and imiquimod-induced mice psoriatic model. Immortalized human epidermal keratinocytes were transduced with a recombinant adenovirus expressing microRNA specific for WWOX to downregulate its expression. Inflammatory responses were detected using Western blotting, real-time quantitative reverse transcription polymerase chain reaction (PCR), and enzyme-linked immunosorbent assay. In human epidermal keratinocytes, WWOX knockdown reduced nuclear factor-kappa B signaling and levels of proinflammatory cytokines induced by polyinosinic: polycytidylic acid [(poly(I:C)] in vitro. Furthermore, calcium chelator and protein kinase C (PKC) inhibitors significantly reduced poly(I:C)-induced inflammatory reactions. WWOX plays a role in the inflammatory reaction of epidermal keratinocytes by regulating calcium and PKC signaling. Targeting WWOX could be a novel therapeutic approach for psoriasis in the future.
Antioxidants and azd0156 Rescue Inflammatory Response in Autophagy-Impaired Macrophages
Authors: Elbialy Abdalla , Kitauchi Mai , Yamanouchi Dai
Published: December 2023

Autophagy is a lysosomal degradation system that eliminates and recycles damaged intracellular organelles and proteins. Inflammatory macrophages play a critical role in the development of various age-related inflammatory illnesses such as abdominal aortic aneurysm, atherosclerosis, and rheumatoid arthritis; therefore, identifying the mechanisms that cause macrophage inflammation is crucial for a better understanding of and developing therapeutics for inflammatory diseases. Previous research has linked autophagy to macrophage inflammation; Atg16L1-deficient macrophages increase IL-1 and IL-18 production via inflammasome activation. In this study, however, we show an alternative pathway of macrophage inflammation in an autophagy-deficient environment. We found that inhibiting autophagy in THP1 macrophages progressively increased the expression of p65-mediated inflammatory genes. This effect was reversed by treatment with antioxidants or azd0156, an ataxia telangiectasia mutated (ATM) inhibitor. In addition, our results showed that M1 macrophages inhibit autophagy and induce DNA damage, whereas M2 macrophages activate autophagy and reduce DNA damage. Importantly, the chemical activation of autophagy or ATM inhibition during M1 polarization reduced the M1 phenotype and inflammation, whereas inhibiting autophagy during M2 polarization also reduced the M2 phenotype. Thus, our findings highlight the importance of the autophagy–ATM pathway in driving macrophage inflammation.
Aberrant Platelet Aggregation as Initial Presentation of Essential Thrombocythemia: Failure of Entero-Coated Aspirin to Reduce Platelet Hyperactivation
Authors: Morotti Alessandro , Barale Cristina , Sornatale Michele , Giugliano Emilia , Muccio Vittorio Emanuele , Frascaroli Chiara , Pautasso Marisa , Fornari Alessandro , Russo Isabella
Published: December 2023

Essential thrombocythemia (ET) is a myeloproliferative neoplasm variant characterized by excessive production of platelets. Since the most common cause of mortality and morbidity in ET patients is thrombosis, the excessive production of platelets may cause thrombotic events. However, little is known about the function of platelets in ET. We report a female patient who presented as asymptomatic, without a remarkable medical history, and ET was diagnosed after an incidental finding of moderate thrombocytosis. Notably, together with thrombocytosis, an abnormal platelet phenotype was found for the presence of a massive, rapid and spontaneous formation of aggregates and platelet hypersensitivity to subthreshold concentrations of aggregating agonists. Bone marrow histopathological examination and genetic analysis with the JAK2 (V617F) gene mutation findings confirmed the initial suspicion of ET. Although the ET patient was placed on aspirin, the persistence of the platelet hyperactivation and hyperaggregability prompted a switch in antiplatelet medication from entero-coated (EC) to plain aspirin. As result, platelet hypersensitivity to agonists and spontaneous aggregation were no longer found. Collectively, our study demonstrates that platelet function analysis could be a reliable predictor of ET and that plain aspirin should be preferred over EC aspirin to attenuate platelet hyperreactivity.
Neuroplastin Expression in Male Mice Is Essential for Fertility, Mating, and Adult Testosterone Levels
Authors: Chen Juanjuan , Lin Xiao , Bhattacharya Soumee , Wiesehöfer Caroline , Wennemuth Gunther , Müller Karin , Montag Dirk
Published: December 2023

Male reproduction depends on hormonally driven behaviors and numerous genes for testis development and spermatogenesis. Neuroplastin-deficient (Nptn−/−) male mice cannot sire offspring. By immunohistochemistry, we characterized neuroplastin expression in the testis. Breeding, mating behavior, hormonal regulation, testicular development, and spermatogenesis were analyzed in cell-type specific neuroplastin mutant mice. Leydig, Sertoli, peritubular myoid, and germ cells express Np, but spermatogenesis and sperm number are not affected in Nptn−/− males. Neuroplastin lack from CNS neurons or restricted to spermatogonia or Sertoli cells permitted reproduction. Normal luteinizing hormone (LH) and follicle-stimulating hormone (FSH) blood levels in Nptn−/− males support undisturbed hormonal regulation in the brain. However, Nptn−/− males lack mounting behavior accompanied by low testosterone blood levels. Testosterone rise from juvenile to adult blood levels is absent in Nptn−/− males. LH-receptor stimulation raising intracellular Ca2+ in Leydig cells triggers testosterone production. Reduced Plasma Membrane Ca2+ ATPase 1 (PMCA1) in Nptn−/− Leydig cells suggests that Nptn−/− Leydig cells produce sufficient testosterone for testis and sperm development, but a lack of PMCA-Np complexes prevents the increase from reaching adult blood levels. Behavioral immaturity with low testosterone blood levels underlies infertility of Nptn−/− males, revealing that Np is essential for reproduction.