bluuebunny/crossref_metadata_2025 huggingface dataset:
A Novel Method for Predicting Ideal Postoperative Upper Instrumented Vertebra Tilt to Prevent Lateral Shoulder Imbalance after Scoliosis Correction Surgery
Authors: Zhang Wen , Xu Mengmeng , Zhang Weimin , Li Tao , Lai Yudong , Chen Fei , Sun Mingtong , Wang Haoyu , Sun Jianmin , Cui Xingang , Jiang Zhensong
Published: February 2023

Lateral shoulder imbalance (LSI) is reflected radiologically by the clavicle angle (CA). How to achieve postoperative lateral shoulder balance (LSB) after scoliosis correction surgery remains unclear. In the current study, by using the preoperative upper instrumented vertebra (UIV) tilt, the CA, the flexibility between T1 and the UIV, and the ideal postoperative UIV tilt was predicted based on the following formula: ideal postoperative UIV tilt = preoperative UIV tilt—the flexibility between T1 and UIV—preoperative CA. The reliability of the formula was verified through a retrospective analysis, and 76 scoliosis patients were enrolled. The feasibility of this method was verified through a prospective analysis, and 13 scoliosis patients were enrolled. In the retrospective study, there was a significant correlation between the difference in the actual and ideal postoperative UIV tilt values and the postoperative CA, with correlation coefficients in the whole, LSI, and LSB groups of 0.981, 0.982, and 0.953, respectively (p < 0.001). In the prospective study, all patients achieved satisfactory LSB. Using the formula preoperatively to predict an ideal postoperative UIV tilt and controlling the intraoperative UIV tilt with the improved crossbar technique may be an effective digital method for achieving postoperative LSB and has important clinical significance.
Association Analysis of Maximum Standardized Uptake Values Based on 18F-FDG PET/CT and EGFR Mutation Status in Lung Adenocarcinoma
Authors: Gao Jianxiong , Shi Yunmei , Niu Rong , Shao Xiaoliang , Shao Xiaonan
Published: February 2023

(1) Background: To investigate the association between maximum standardized uptake value (SUVmax) based on 18F-FDG PET/CT and EGFR mutation status in lung adenocarcinoma. (2) Methods: A total of 366 patients were retrospectively collected and divided into the EGFR mutation group (n = 228) and EGFR wild-type group (n = 138) according to their EGFR mutation status. The two groups’ general information and PET/CT imaging parameters were compared. A hierarchical binary logistic regression model was used to assess the interaction effect on the relationship between SUVmax and EGFR mutation in different subgroups. Univariate and multivariate logistic regression was used to analyze the association between SUVmax and EGFR mutation. After adjusting for confounding factors, a generalized additive model and smooth curve fitting were applied to address possible non-linearities. (3) Results: Smoking status significantly affected the relationship between SUVmax and EGFR mutation (p for interaction = 0.012), with an interaction effect. After adjusting for age, gender, nodule type, bronchial sign, and CEA grouping, in the smoking subgroup, curve fitting results showed that the relationship between SUVmax and EGFR mutation was approximately linear (df = 1.000, c2 = 3.897, p = 0.048); with the increase in SUVmax, the probability of EGFR mutation gradually decreased, and the OR value was 0.952 (95%CI: 0.908–0.999; p = 0.045). (4) Conclusions: Smoking status can affect the relationship between SUVmax and EGFR mutation status in lung adenocarcinoma, especially in the positive smoking history subgroup. Fully understanding the effect of smoking status will help to improve the accuracy of SUVmax in predicting EGFR mutations.
Discontinuing Hepatitis Activity Reduced Hepatocellular Carcinoma Recurrence after Primary Curative Therapy
Authors: Lee Chern-Horng , Shen Chien-Heng , Yen Cho-Li , Yen Tzung-Hai , Hsieh Sen-Yung
Published: February 2023

Background: Hepatocellular carcinoma (HCC) tends to recur after curative treatment. This study aimed to identify the clinical factors associated with HCC recurrence after initial curative therapy. Methods: We retrospectively included patients with early stage HCC Barcelona Clinic Liver Cancer (BCLC) stages 0 and A who received curative surgical resection or local ablation at three different Chang Gung Memorial Hospitals in Taiwan (527 patients from Linkou, 150 patients from Keelung, and 127 patients from Chiayi) from 2000 to 2009. Pretreatment clinical data were subjected to univariate and multivariate logistic analyses to identify the risk factors for HCC recurrence within five years after the primary curative treatment. Recurrence and survival rates were assessed using Kaplan–Meier curves and log-rank tests. Results: Patients with a history of nucleoside analog or peg-interferon treatment for hepatitis B or hepatitis C infection had lower HCC recurrence rates than did those without such treatment. By contrast, alcohol drinking habits (p = 0.0049, hazard ratio (HR): 1.508, 95%CI: 1.133–2.009), a platelet count of < 14 × 104/μL (p = 0.003, HR: 1.533, 95%CI: 1.155–2.035), and a serum alanine aminotransferase level > 40 U/L (p = 0.0450, HR: 1.305, 95%CI: 1.006–1.694) were independent risk factors for HCC recurrence. The five-year HCC recurrence rates did not differ between patients who received either local radiofrequency ablation or surgical resection at BCLC stages 0 and A. Conclusions: Factors contributing to persistent hepatitis activity and advanced fibrosis precipitate tumor recurrence. Active intervention to discontinue liver injury or hepatitis could reduce HCC recurrence.
Different Pediatric Acute Care Settings Influence Bronchiolitis Management: A 10-Year Retrospective Study
Authors: Biagi Carlotta , Betti Ludovica , Manieri Elisa , Dondi Arianna , Pierantoni Luca , Ramanathan Ramsiya , Zama Daniele , Gennari Monia , Lanari Marcello
Published: February 2023

Bronchiolitis is the main cause of hospitalization in infants. Diagnosis is clinical, and treatment is based on hydration and oxygen therapy. Nevertheless, unnecessary diagnostic tests and pharmacological treatments are still very common. This retrospective study aimed to evaluate whether the setting of bronchiolitis care influences diagnostic and therapeutic choices. The management of 3201 infants, referred to our Italian Tertiary Care Center for bronchiolitis between 2010 and 2020, was analyzed by comparing children discharged from the pediatric emergency department (PEDd group) undergoing short-stay observation (SSO group) and hospitalization. Antibiotic use in PEDd, SSO, and ward was 59.3% vs. 51.6% vs. 49.7%, respectively (p < 0.001); inhaled salbutamol was mainly administered in PEDd and during SSO (76.1% and 82.2% vs. 38.3% in ward; p < 0.001); the use of corticosteroids was higher during SSO and hospitalization (59.6% and 49.1% vs. 39.0% in PEDd; p < 0.001); inhaled adrenaline was administered mostly in hospitalized infants (53.5% vs. 2.5% in SSO and 0.2% in PEDd; p < 0.001); chest X-ray use in PEDd, SSO, and ward was 30.3% vs. 49.0% vs. 70.5%, respectively (p < 0.001). In a multivariate analysis, undergoing SSO was found to be an independent risk factor for the use of systemic corticosteroid and salbutamol; being discharged at home was found to be a risk factor for antibiotic prescription; undergoing SSO and hospitalization resulted as independent risk factors for the use of CXR. Our study highlights that different pediatric acute care settings could influence the management of bronchiolitis. Factors influencing practice may include a high turnover of PED medical staff, personal reassurance, and parental pressure.
Impact of a Static Magnetic Field on Early Osseointegration: A Pilot Study in Canines
Authors: Michels Roberta , Kampleitner Carina , Dobsak Toni , Doppelmayer Kevin , Heimel Patrick , Lettner Stefan , Tangl Stefan , Gruber Reinhard , Benfatti César Augusto Magalhães
Published: February 2023

A static magnetic field generated by neodymium–iron–boron (NdFeB) magnets placed in the inner cavity of dental implants can enhance bone regeneration in rabbits. It is, however, unknown whether static magnetic fields support osseointegration in a canine model. We therefore determined the potential osteogenic effect of implants carrying NdFeB magnets inserted in the tibia of six adult canines in the early stages of osseointegration. Here, we report that after 15 days of healing, magnetic and regular implants showed a high variation with a median new bone-to-implant contact (nBIC) in the cortical (41.3% and 7.3%) and the medullary (28.6% and 44.8%) region, respectively. Consistently, the median new bone volume/tissue volume (nBV/TV) in the cortical (14.9% and 5.4%) and the medullary (22.2% and 22.4%) region were not significantly different. One week of healing only resulted in negligible bone formation. These findings suggest that considering the large variation and the pilot nature of this study, magnetic implants failed to support peri-implant bone formation in a canine model.
Development of Three-Layered Composite Color Converters for White LEDs Based on the Epitaxial Structures of YAG:Ce, TbAG:Ce and LuAG:Ce Garnets
Authors: Markovskyi Anton , Gorbenko Vitaliy , Zorenko Tatiana , Witkiewicz-Lukaszek Sandra , Sidletskiy Oleg , Fedorov Alexander , Zorenko Yuriy
Published: February 2023

This work was dedicated to the development of novel types of composite phosphor converters of white LED, based on the epitaxial structures containing Y3Al5O12:Ce (YAG:Ce) and Tb3Al5O12:Ce (TbAG:Ce) single crystalline films, steeply grown, using the liquid-phase epitaxy method, onto LuAG:Ce single crystal substrates. The influence of Ce3+ concentration in the LuAG:Ce substrate, as well as the thickness of the subsequent YAG:Ce and TbAG:Ce films, on the luminescence and photoconversion properties of the three-layered composite converters were investigated. Compared to its traditional YAG:Ce counterpart, the developed composite converter demonstrates broadened emission bands, due to the compensation of the cyan–green dip by the additional LuAG:Ce substrate luminescence, along with yellow–orange luminescence from the YAG:Ce and TbAG:Ce films. Such a combination of emission bands from various crystalline garnet compounds allows the production of a wide emission spectrum of WLEDs. In turn, the variation in the thickness and activator concentration in each part of the composite converter allows the production of almost any shade from green to orange emission on the chromaticity diagram.
Reduction in Errors in Roughness Evaluation with an Accurate Definition of the S-L Surface
Authors: Podulka Przemysław , Macek Wojciech , Branco Ricardo , Nejad Reza Masoudi
Published: February 2023

Characterization of surface topography, roughly divided into measurement and data analysis, can be valuable in the process of validation of the tribological performance of machined parts. Surface topography, especially the roughness, can respond straightly to the machining process and, in some cases, is defined as a fingerprint of the manufacturing. When considering the high precision of surface topography studies, the definition of both S-surface and L-surface can drive many errors that influence the analysis of the accuracy of the manufacturing process. Even if precise measuring equipment (device and method) is provided but received data are processed erroneously, the precision is still lost. From that matter, the precise definition of the S-L surface can be valuable in the roughness evaluation allowing a reduction in the rejection of properly made parts. In this paper, it was proposed how to select an appropriate procedure for the removal of the L- and S- components from the raw measured data. Various types of surface topographies were considered, e.g., plateau-honed (some with burnished oil pockets), turned, milled, ground, laser-textured, ceramic, composite, and, generally, isotropic. They were measured with different (stylus and optical) methods, respectively, and parameters from the ISO 25178 standard were also taken into consideration. It was found that commonly used and available commercial software methods can be valuable and especially helpful in the precise definition of the S-L surface; respectively, its usage requires an appropriate response (knowledge) from the users.
Process Optimization of the Hot Stamping of AZ31 Magnesium Alloy Sheets Based on Response Surface Methodology
Authors: Zhao Pengjing , Wu Qi , Yang Yo-Lun , Chen Zhanghua
Published: February 2023

Hot stamping is an important manufacturing process for sheet metal parts. However, it is easy to produce defects such as thinning and cracking in the drawing area during the stamping process. In this paper, the finite element solver ABAQUS/Explicit was used to establish the numerical model of the magnesium alloy hot-stamping process. The stamping speed (2~10 mm/s), the blank-holder force (3~7 kN), and the friction coefficient (0.12~0.18) were selected as the influencing factors. Taking the maximum thinning rate obtained through simulation as the optimization objective, the response surface methodology (RSM) was applied to optimize the influencing factors in sheet hot stamping at a forming temperature of 200 °C. The results showed that the maximum thinning rate of sheet metal was most influenced by the blank-holder force, and the interaction between the stamping speed and the blank-holder force/friction coefficient had a great influence on the maximum thinning rate. The optimal value of the maximum thinning rate of the hot-stamped sheet was 7.37%. Through the experimental verification for the hot-stamping process scheme, the maximum relative error between the simulation and the experimental results was 8.72%. This proves the accuracy of the established finite element model and the response surface model. This research provides a feasible optimization scheme for the analysis of the hot-stamping process of magnesium alloys.
Single Crystalline Films of Ce3+-Doped Y3MgxSiyAl5−x−yO12 Garnets: Crystallization, Optical, and Photocurrent Properties
Authors: Gorbenko Vitaliy , Zorenko Tetiana , Shakhno Anna , Popielarski Paweł , Osvet Andres , Batentschuk Miroslaw , Fedorov Alexander , Mahlik Sebastian , Leśniewski Tadeusz , Majewska Natalia , Zorenko Yuriy
Published: February 2023

This research focuses on LPE growth, and the examination of the optical and photovoltaic properties of single crystalline film (SCF) phosphors based on Ce3+-doped Y3MgxSiyAl5−x−yO12 garnets with Mg and Si contents in x = 0–0.345 and y = 0–0.31 ranges. The absorbance, luminescence, scintillation, and photocurrent properties of Y3MgxSiyAl5−x−yO12:Ce SCFs were examined in comparison with Y3Al5O12:Ce (YAG:Ce) counterpart. Especially prepared YAG:Ce SCFs with a low (x, y < 0.1) concentration of Mg2+ and Mg2+–Si4+ codopants also showed a photocurrent that increased with rising Mg2+ and Si4+ concentrations. Mg2+ excess was systematically present in as-grown Y3MgxSiyAl5−x−yO12:Ce SCFs. The as-grown SCFs of these garnets under the excitation of α–particles had a low light yield (LY) and a fast scintillation response with a decay time in the ns range due to producing the Ce4+ ions as compensators for the Mg2+ excess. The Ce4+ dopant recharged to the Ce3+ state after SCF annealing at T > 1000 °C in a reducing atmosphere (95%N2 + 5%H2). Annealed SCF samples exhibited an LY of around 42% and similar scintillation decay kinetics to those of the YAG:Ce SCF counterpart. The photoluminescence studies of Y3MgxSiyAl5−x−yO12:Ce SCFs provide evidence for Ce3+ multicenter formation and the presence of an energy transfer between various Ce3+ multicenters. The Ce3+ multicenters possessed variable crystal field strengths in the nonequivalent dodecahedral sites of the garnet host due to the substitution of the octahedral positions by Mg2+ and the tetrahedral positions by Si4+. In comparison with YAG:Ce SCF, the Ce3+ luminescence spectra of Y3MgxSiyAl5−x−yO12:Ce SCFs greatly expanded in the red region. Using these beneficial trends of changes in the optical and photocurrent properties of Y3MgxSiyAl5−x−yO12:Ce garnets as a result of Mg2+ and Si4+ alloying, a new generation of SCF converters for white LEDs, photovoltaics, and scintillators could be developed.
In Vitro Testing and Clinical Handling of a Novel Implant Positioning Technology for Proximal Humeral Plating
Authors: Windolf Markus , Knierzinger Dominik , Nijs Stefaan , Sermon An , Blauth Michael , Richards Robert Geoff , Buschbaum Jan
Published: February 2023

Background and Objectives: Fractures of the proximal humerus are common, particularly in elderly populations. Anatomical locking plates target stabilization with a multitude of screws spanning into the humeral head. Sound implant placement and screw length determination are key for a successful clinical outcome but are difficult to obtain from planar X-rays. A novel implant positioning technology for proximal humerus plating (Xin1) outputs screw lengths suggestions and plate position based on hole projections in conventional X-ray images. This study investigated the performance of a prototype Xin1 system in a postmortem (in vitro) experiment as well as in a clinical handling test. Materials and Methods: For in vitro testing, twelve shoulders from six anatomical specimens were randomized into two groups to compare the Xin1 technique to the conventional operation in terms of surgical precision, procedure time and X-ray exposure. For the clinical trial, 11 patients undergoing plating of the proximal humerus were included. The aim was to investigate clinical handling of the Xin1 marker clip and to retrospectively evaluate the system performance in a real-life fracture situation. Image pairs before and after insertion of the proximal screws were retrospectively processed to investigate the influence of potential bone fragment shifts on the system output. Results: In the postmortem experiment, the use of the system significantly improved the surgical precision (52% error reduction), procedure time (38% shorter) and radiation exposure (64% less X-rays). Clinical handling demonstrated seamless embedding of the marker clip into existing clinical workflows without adverse events reported. Retrospective X-ray analysis on six eligible patients revealed differences in the calculated screw lengths of ≤2 mm before and after screw insertion for five patients. In one patient, the screw lengths differed up to 8 mm, which might indicate displacement of the head fragment. Conclusions: Results suggest a strong potential of the Xin1 assistance technology to enhance the surgical procedure and patient outcomes in the rising incidence of osteoporotic humeral fractures. Robust performance in a real-life fracture situation was observed. In-depth validation of the system is, however, needed before placing it into clinical practice.
The Role of Iron-Chelating Therapy in Improving Neurological Outcome in Patients with Intracerebral Hemorrhage: Evidence-Based Case Report
Authors: Ramadhan Mochamad Iskandarsyah Agung , Sitanaya Shierly Novitawati , Hakim Ariadri Hafian Wulandaru , Ramli Yetty
Published: February 2023

Current primary intracerebral hemorrhage (ICH) treatments focus on limiting hematoma volume by lowering blood pressure, reversing anticoagulation, or hematoma evacuation. Nevertheless, there is no effective strategy to protect the brain from secondary injury due to ICH. Excess heme and iron as by-products of lysing clots in ICH might contribute to this secondary injury by triggering perihematomal edema. We present a clinical situation of an ICH case where iron-chelating therapy might be beneficial, as supported by scientific evidence. We looked through four databases (Pubmed, Cochrane, Embase, and Google Scholar) to find studies assessing the efficacy of iron-chelating therapy in ICH patients. Validity, importance, and applicability (VIA) of the included articles were appraised using worksheets from the Oxford Centre for Evidence-Based Medicine. Two out of five eligible studies were valid, important, and applicable to our patient. Both studies showed the positive effects of iron-chelating therapy on neurological outcome, as measured by National Institutes of Health Stroke Scale (NIHSS) score and modified Rankin Score (mRS). The beneficial effects of deferoxamine were demonstrated within the moderate volume (10–30 mL) subgroup, with a positive relative risk reduction (RRR) and low number needed to treat (six persons). Based on our appraisal, we considered iron-chelating therapy as an additional therapy for ICH patients, given its benefits and adverse effects. More specific studies using a larger sample size, focusing on moderate-volume ICH, and using standardized neurological outcomes are encouraged.
Mapping the Metabolic Niche of Citrate Metabolism and SLC13A5
Authors: Chen Fangfang , Willenbockel Hanna Friederike , Cordes Thekla
Published: February 2023

The small molecule citrate is a key molecule that is synthesized de novo and involved in diverse biochemical pathways influencing cell metabolism and function. Citrate is highly abundant in the circulation, and cells take up extracellular citrate via the sodium-dependent plasma membrane transporter NaCT encoded by the SLC13A5 gene. Citrate is critical to maintaining metabolic homeostasis and impaired NaCT activity is implicated in metabolic disorders. Though citrate is one of the best known and most studied metabolites in humans, little is known about the consequences of altered citrate uptake and metabolism. Here, we review recent findings on SLC13A5, NaCT, and citrate metabolism and discuss the effects on metabolic homeostasis and SLC13A5-dependent phenotypes. We discuss the “multiple-hit theory” and how stress factors induce metabolic reprogramming that may synergize with impaired NaCT activity to alter cell fate and function. Furthermore, we underline how citrate metabolism and compartmentalization can be quantified by combining mass spectrometry and tracing approaches. We also discuss species-specific differences and potential therapeutic implications of SLC13A5 and NaCT. Understanding the synergistic impact of multiple stress factors on citrate metabolism may help to decipher the disease mechanisms associated with SLC13A5 citrate transport disorders.
Association between Tryptophan Metabolism and Inflammatory Biomarkers in Dairy Cows with Ketosis
Authors: Luo Zhengzhong , Yong Kang , Du Zhenlong , Huang Yixin , Zhou Tao , Ma Li , Yao Xueping , Shen Liuhong , Yu Shumin , Yan Zuoting , Cao Suizhong
Published: February 2023

Dairy cows with ketosis have high circulating beta-hydroxybutyric acid (BHBA) concentrations alongside which inflammation is concomitantly developed. Tryptophan (Trp) is an essential amino acid that participates in the regulation of the inflammatory response. However, the association between Trp metabolism and inflammation in dairy cows with ketosis remains unclear. Therefore, blood samples from healthy (n = 10) and ketotic (n = 10) primiparous dairy cows were collected at the calving date and the day of ketosis diagnosis (7 days in milk (7 DIM)). Serum levels of non-esterified fatty acids (NEFA), BHBA, haptoglobin (HP), serum amyloid A (SAA), lipopolysaccharide, and cortisol were analyzed. Tryptophan and its metabolites were quantified using liquid chromatography–tandem mass spectrometry. At 7 DIM, the concentrations of NEFA, BHBA, HP, and SAA were higher and the levels of Trp, kynurenine (KYN), indoleacetic acid, indole-3-lactic acid, and 3-indoxyl sulfate were lower in the dairy cows with ketosis compared with those in the healthy cows. However, the KYN/Trp and melatonin/Trp ratios increased in the cows with ketosis. At the calving date, the serum lipopolysaccharide levels did not differ between the healthy and ketotic cows, whereas the levels of NEFA, HP, and cortisol increased in the ketotic cows. Correlation analysis showed that Trp deficiency and elevated Trp metabolism in the dairy cows occurred during ketosis. Overall, our results suggest that abnormal Trp metabolism may contribute to the pathogenesis of ketosis.
Dietary Supplementation of Chestnut Tannins in Prepartum Dairy Cows Improves Antioxidant Defense Mechanisms Interacting with Thyroid Status
Authors: Prodanović Radiša , Nedić Sreten , Vujanac Ivan , Bojkovski Jovan , Nedić Svetlana , Jovanović Ljubomir , Kirovski Danijela , Borozan Sunčica
Published: February 2023

Cows in the peripartal period undergo changes in thyroid hormones and are susceptible to lipomobilization and/or oxidative stress. The addition of chestnut tannins as polyphenolic compounds in the diet may improve feed efficiency and prevent oxidative stress-related health disorders in transition cows. However, the relationship between chestnut tannin supplementation and thyroid function, which plays an important role in metabolic regulation, has not been investigated in dairy cows. This study was conducted to investigate the effects of chestnut tannin supplementation during the close-up period on thyroid status and to evaluate the interaction between thyroid hormones and oxidative stress biomarkers in prepartum dairy cows. Forty multiparous Holstein cows were fed either a diet containing chestnut tannins (CNTs, n = 20, 1.96 g chestnut tannins/kg feed, dry matter) or a non-supplemented diet (CON, n = 20) during the last 25 ± 2 days of gestation. Blood samples were collected on the first day of study (before chestnut tannin supplementation) and d 5 before parturition to measure hormonal and oxidative stress indices. Serum concentrations of T3 (p = 0.04) and T4 (p = 0.05) were higher in CNT cows than in the CON group on day 5 before parturition. Thyroid status of CNT cows was associated with higher serum total antioxidant capacity (T-AOC, p < 0.01), activities of superoxide dismutase (SOD, p = 0.03) and glutathione peroxidase (GPx, p = 0.01), and reduced glutathione concentration (GSH, p = 0.05). Serum thiobarbituric acid reactive substances (TBARS) were lower (p = 0.04) which was associated with lower aspartate aminotransferase (AST, p = 0.02), and lactate dehydrogenase (LDH, p = 0.01) activities in the CNT than in the CON group. Estradiol and progesterone did not differ between CNT and CON cows. Chestnut tannin supplementation improves antioxidant protection, prevents oxidation-reduction processes, reduces the degree of liver cell membrane damage, and protects thyroid tissue from damage, allowing higher T3 and T4 synthesis. Considering the importance of the thyroid hormone status before parturition, mechanisms of thyroid hormone regulation in CNT-supplemented dairy cows require more detailed investigations.
Metabolomics Provides New Insights into Host Manipulation Strategies by Asobara japonica (Hymenoptera: Braconidae), a Fruit Fly Parasitoid
Authors: Liu Shengmei , Zhang Junwei , Sheng Yifeng , Feng Ting , Shi Wenqi , Lu Yueqi , Guan Xueying , Chen Xuexin , Huang Jianhua , Chen Jiani
Published: February 2023

Asobara japonica (Hymenoptera: Braconidae) is an endoparasitoid wasp that can successfully parasitize a wide range of host species across the Drosophila genus, including the invasive crop pest Drosophila suzukii. Parasitoids are capable of regulating the host metabolism to produce the nutritional metabolites for the survival of their offspring. Here, we intend to investigate the metabolic changes in D. melanogaster hosts after parasitization by A. japonica, using the non-targeted LC-MS (liquid chromatography-mass spectrometry) metabolomics analysis. In total, 3043 metabolites were identified, most of which were not affected by A. japonica parasitization. About 205 metabolites were significantly affected in parasitized hosts in comparison to non-parasitized hosts. The changed metabolites were divided into 10 distinct biochemical groups. Among them, most of the lipid metabolic substances were significantly decreased in parasitized hosts. On the contrary, most of metabolites associated with the metabolism of amino acids and sugars showed a higher abundance of parasitized hosts, and were enriched for a wide range of pathways. In addition, eight neuromodulatory-related substances were upregulated in hosts post A. japonica parasitization. Our results reveal that the metabolites are greatly changed in parasitized hosts, which might help uncover the underlying mechanisms of host manipulation that will advance our understanding of host–parasitoid coevolution.
Spatial Analysis of Structure and Metal Mineralization Based on Fractal Theory and Fry Analysis: A Case Study in Nenjiang−Heihe Metallogenic Belt
Authors: Zhao Zhonghai , Chen Jun , Cheng Binbin , Liu Yiwen , Qiao Kai , Cui Xiaomeng , Yin Yechang , Li Chenglu
Published: February 2023

Regional tectonics can provide excellent transport channels and precipitation sites for mineralized hydrothermal fluid. Studying the spatial relationship and distribution trends of regional tectonics and metal mineralization has theoretical and practical significance for revealing regional mineralization regularities and guiding mineral exploration. This study considers the Nenjiang-Heihe metallogenic belt, through the fractal box dimension method and Fry analysis, to explore the spatial distribution characteristics and patterns of tectonics. The results were as follows. (1) NE and NW directions are the main tectonic directions in the study area, with high-density areas concentrated in the central-eastern and central-western regions, demonstrating an overall ring-like distribution pattern. (2) Fractal dimensions of the linear structures of the NE and NW directions and the entire study area are 1.543, 1.493, and 1.622, respectively, with a strong coupling relationship between the lineament fractal high-value area and rhombic-grid spatial distribution of known deposits. (3) Gold mineralization shows the NEE and NWW directions as two main mineralization trends; the intersection area is the gold-potential area. The main trend direction of the Cu-Mo metallogenic trend belt is the NNW direction; the intersection area with the NEE direction gold metallogenic trend belt is the Au-Cu-Mo potential mineralization area.
Performance Comparison of the Vertical and Horizontal Oriented Stirred Mill: Pilot Scale IsaMill vs. Full-Scale HIGMill
Authors: Can Metin , Altun Okay
Published: February 2023

Varied types/geometries of stirred mills have been produced by different manufacturers, and the comparison task has been accomplished for some of the technologies, i.e., Tower mill vs IsaMill. However, the main drawbacks of these comparisons were the uncommon characteristics of the milling environment, such as media size. In this study, HIGMill and IsaMill, which were vertically and horizontally chamber oriented, respectively, were compared for a regrinding process of copper ores with similar characterization and almost the same milling environment. Detailed characterization studies of the two ore types, such as work index, ore breakage and chemical composition, were performed. Modeling of the two mills was also performed to show the variation in the rate of breakage parameters. The entire assessments were based on comparing the signature plots, energy and shape of the product size distribution as well as the stress analyses. The results showed that HIGMill and IsaMill technologies behaved in a different manner for coarse and fine tail of comminution. IsaMill with horizontal orientation was found to be more energy-efficient, particularly at the fine grind size, and produced finer product when it was operated at the same stress level of HIGMill.
Improving Nickel Recovery in Froth Flotation by Purifying Concentrators Process Water Using Dissolved Air Flotation
Authors: Aaltonen Annukka , Le Thi Minh Khanh , Saari Eija , Dahl Olli , Musuku Benjamin , Lang Aleksandra , Hiidenheimo Sakari , Dixon Richard
Published: February 2023

The pressure for saving water by closing the water loops in mineral processing is increasing continuously. The drivers for higher recirculating rates include water scarcity in dry areas, environmental legislation that is becoming stricter in most countries, limitations set for wet tailings management and the increased demands for social licenses to operate. At the same time, to make mineral processing sustainable, the recovery of valuable minerals should be maximized. This leads for a need to close the process water circulation. To see the effect of closed water circulation on metallurgical performance, flotation tests were carried out with nickel concentrate thickener overflow water before and after the process of water purification by dissolved air flotation (DAF). Both total nickel recovery and concentrate grade in laboratory scale flotation tests to the Ni rougher-scavenger concentrate increased after DAF treatment. Chemical and mineralogical characterizations revealed that after DAF treatment, the process water contained fewer metal hydroxides and less fine-grained silicate mineral particles, which is most likely the reason for the improvement in the nickel flotation performance. Based on the feasibility study, improved nickel recovery by DAF treatment of process water can bring economic benefits at a concentrator plant.
Improving the Performance of a Triboelectric Nanogenerator by Using an Asymmetric TiO2/PDMS Composite Layer
Authors: Zhou Qingyang , Takita Ryuto , Ikuno Takashi
Published: February 2023

To improve the output power of the polydimethylsiloxane (PDMS)-based triboelectric nanogenerators (TENGs), we fabricated an asymmetric TiO2/PDMS composite film in which a pure PDMS thin film was deposited as a capping layer on a TiO2 nanoparticles (NPs)-embedded PDMS composite film. Although in the absence of the capping layer, the output power decreased when the content of TiO2 NPs exceeded a certain value, the asymmetric TiO2/PDMS composite films showed that the output power increased with increasing content. The maximum output power density was approximately 0.28 W/m2 at a TiO2 content of 20 vol.%. The capping layer could be responsible not only for maintaining the high dielectric constant of the composite film but also for suppressing interfacial recombination. To further improve the output power, we applied a corona discharge treatment to the asymmetric film and measured the output power at a measurement frequency of 5 Hz. The maximum output power density was approximately 78 W/m2. The idea of the asymmetric geometry of the composite film should be applicable to various combinations of materials for TENGs.
ZIF-8@Rhodamine B as a Self-Reporting Material for Pollutant Extraction Applications
Authors: Moore Edward , Maya Fernando
Published: February 2023

Herein, we have evaluated the potential of dye-encapsulation as a simple mechanism to self-report the stability of MOFs for pollutant extraction applications. This enabled the visual detection of material stability issues during the selected applications. As proof-of-concept, the zeolitic imidazolate framework (ZIF-8) material was prepared in aqueous medium and at room temperature in the presence of the dye rhodamine B. The total amount of loaded rhodamine B was determined using UV-vis spectrophotometry. The prepared dye-encapsulated ZIF-8 showed a comparable extraction performance with bare ZIF-8 for the removal of hydrophobic endocrine-disrupting phenols, such as 4-tert-octylphenol and 4-nonylphenol, and improved the extraction performance of more hydrophilic endocrine disruptors, such as bisphenol A and 4-tert-butylphenol.
Multi-Compartmental Dissolution Method, an Efficient Tool for the Development of Enhanced Bioavailability Formulations Containing Poorly Soluble Acidic Drugs
Authors: Katona Miklós Tamás , Nagy-Katona Lili , Szabó Réka , Borbás Enikő , Tonka-Nagy Péter , Takács-Novák Krisztina
Published: February 2023

The purpose of this study was to investigate the applicability of the Gastrointestinal Simulator (GIS), a multi-compartmental dissolution model, to predict the in vivo performance of Biopharmaceutics Classification System (BCS) Class IIa compounds. As the bioavailability enhancement of poorly soluble drugs requires a thorough understanding of the desired formulation, the appropriate in vitro modelling of the absorption mechanism is essential. Four immediate release ibuprofen 200 mg formulations were tested in the GIS using fasted biorelevant media. In addition to the free acid form, ibuprofen was present as sodium and lysine salts in tablets and as a solution in soft-gelatin capsules. In the case of rapid-dissolving formulations, the dissolution results indicated supersaturation in the gastric compartment, which affected the resulting concentrations in the duodenum and the jejunum as well. In addition, a Level A in vitro–in vivo correlation (IVIVC) model was established using published in vivo data, and then the plasma concentration profiles of each formulation were simulated. The predicted pharmacokinetic parameters were consistent with the statistical output of the published clinical study. In conclusion, the GIS method was found to be superior compared to the traditional USP method. In the future, the method can be useful for formulation technologists to find the optimal technique to enhance the bioavailability of poorly soluble acidic drugs.
Cover Crops Modulate the Response of Arbuscular Mycorrhizal Fungi to Water Supply: A Field Study in Corn
Authors: Tosi Micaela , Ogilvie Cameron M. , Spagnoletti Federico N. , Fournier Sarah , Martin Ralph C. , Dunfield Kari E.
Published: February 2023

Cover crops (CCs) were found to improve soil health by increasing plant diversity and ground cover. They may also improve water supply for cash crops by reducing evaporation and increasing soil water storage capacity. However, their influence on plant-associated microbial communities, including symbiotic arbuscular mycorrhizal fungi (AMF), is less well understood. In a corn field trial, we studied the response of AMF to a four-species winter CC, relative to a no-CC control, as well as to two contrasting water supply levels (i.e., drought and irrigated). We measured AMF colonization of corn roots and used Illumina MiSeq sequencing to study the composition and diversity of soil AMF communities at two depths (i.e., 0–10 and 10–20 cm). In this trial, AMF colonization was high (61–97%), and soil AMF communities were represented by 249 amplicon sequence variants (ASVs) belonging to 5 genera and 33 virtual taxa. Glomus, followed by Claroideoglomus and Diversispora (class Glomeromycetes), were the dominant genera. Our results showed interacting effects between CC treatments and water supply levels for most of the measured variables. The percentage of AMF colonization, arbuscules, and vesicles tended to be lower in irrigated than drought sites, with significant differences detected only under no-CC. Similarly, soil AMF phylogenetic composition was affected by water supply only in the no-CC treatment. Changes in the abundance of individual virtual taxa also showed strong interacting effects between CCs, irrigation, and sometimes soil depth, although CC effects were clearer than irrigation effects. An exception to these interactions was soil AMF evenness, which was higher in CC than no-CC, and higher under drought than irrigation. Soil AMF richness was not affected by the applied treatments. Our results suggest that CCs can affect the structure of soil AMF communities and modulate their response to water availability levels, although soil heterogeneity could influence the final outcome.
Effect of Sodium Selenate and Selenocystine on Savoy Cabbage Yield, Morphological and Biochemical Characteristics under Chlorella Supply
Authors: Antoshkina Marina , Golubkina Nadezhda , Poluboyarinov Pavel , Skrypnik Liubov , Sekara Agnieszka , Tallarita Alessio , Caruso Gianluca
Published: February 2023

Biofortification of Brassica oleracea with selenium (Se) is highly valuable both for human Se status optimization and functional food production with direct anti-carcinogenic activity. To assess the effects of organic and inorganic Se supply for biofortifying Brassica representatives, foliar applications of sodium selenate and selenocystine (SeCys2) were performed on Savoy cabbage treated with the growth stimulator microalgae Chlorella. Compared to sodium selenate, SeCys2 exerted a stronger growth stimulation of heads (1.3 against 1.14 times) and an increase of leaf concentration of chlorophyll (1.56 against 1.2 times) and ascorbic acid (1.37 against 1.27 times). Head density was reduced by 1.22 times by foliar application of sodium selenate and by 1.58 times by SeCys2. Despite the greater growth stimulation effect of SeCys2, its application resulted in lower biofortification levels (2.9 times) compared to sodium selenate (11.6 times). Se concentration decreased according to the following sequence: leaves > roots > head. The antioxidant activity (AOA) was higher in water extracts compared to the ethanol ones in the heads, but the opposite trend was recorded in the leaves. Chlorella supply significantly increased the efficiency of biofortification with sodium selenate (by 1.57 times) but had no effect in the case of SeCys2 application. Positive correlations were found between leaf and head weight (r = 0.621); head weight and Se content under selenate supply (r = 0.897–0.954); leaf ascorbic acid and total yield (r = 0.559), and chlorophyll (r = +0.83–0.89). Significant varietal differences were recorded for all the parameters examined. The broad comparison performed between the effects of selenate and SeCys2 showed significant genetic differences as well as important peculiarities connected with the Se chemical form and its complex interaction with Chlorella treatment.
Yield and Grain Quality of Common Wheat (Triticum aestivum L.) Depending on the Different Farming Systems (Organic vs. Integrated vs. Conventional)
Authors: Mitura Katarzyna , Cacak-Pietrzak Grażyna , Feledyn-Szewczyk Beata , Szablewski Tomasz , Studnicki Marcin
Published: February 2023

Genotype (cultivar), soil and climatic conditions, the agrotechnology used, and the interaction of the factors mentioned play a key role in the yield and quality of wheat grain. Currently, the European Union recommends the balanced use of mineral fertilisers and plant protection products in agricultural production (integrated production) or the use of only natural production methods (organic production). The aim of the study was to compare the yield and grain quality of four spring common wheat cultivars Harenda, Kandela, Mandaryna, and Serenada, grown under three farming systems: organic (ORG), integrated (INT), and conventional (CONV). A three-year field experiment was conducted between 2019 and 2021 at the Osiny Experimental Station (Poland, 51°27′ N; 22°2′ E). The results showed that significantly the highest wheat grain yield (GY) was obtained at INT, while the lowest was obtained at ORG. The physicochemical and rheological characteristics of the grain were significantly influenced by the cultivar factor and, with the exception of 1000 grain weight (TGW) and ash content (AC), by the farming system. There were also numerous interactions between the cultivar and farming systems, which suggests different performances of cultivars and, in fact, that some cultivars are better or worse suited to different production systems. The exceptions were protein content (PC) and falling number (FN), which were significantly highest in grain with CONV and lowest in grain with ORG farming systems.
Heteroblastic Inflorescence of Lamium amplexicaule L. in Egyptian Flora
Authors: Amer Wafaa M. , Al Shaye Najla A. , Hassan Mahmoud O. , Khalaf Maha H.
Published: February 2023

Lamium amplexicaule L. (Family: Lamiaceae) is a cosmopolitan weed whose eradication is challenging. The phenoplasticity of this species is related to its heteroblastic inflorescence, which has not received adequate research worldwide in its morphological and genetic aspects. This inflorescence hosts two flower types, a cleistogamous (CL: closed flower) and a chasmogamous (CH: opened flower). This species subjected to detailed investigation is a model species to clarify: (1) the existence of the CL and CH flowers in relation to the time and individual plants. (2) the predominant flower morphs in Egypt. (3) the morphological and genetic variability between these morphs. Among the novel data retrieved from this work is the Presence of this species in three distinct morphs coexisting during winter. These morphs showed remarkable phenoplasticity, particularly in flower organs. Significant differences were observed between the three morphs in pollen fertility, nutlets productivity and sculpture, flowering time, and seed viability. These differences were extended to the genetic profile of these three morphs assessed by the inter simple sequence repeats (ISSRs) and start codon targeted (SCoT). This work highlights the urgent need to study the heteroblastic inflorescence of crop weeds to facilitate its eradication.
Effects of Sugarcane Leaf Return and Fertilizer Reduction on Maize Growth, Yield and Soil Properties in Red Soil
Authors: Liu Yufeng , Tan Yumo , Liang Dan , Pei Chengruo , Zhang Zhenhua
Published: February 2023

In order to make better use of the vast sugarcane leaf straw resources and reduce the overuse of chemical fertilizers in the subtropical red soil region of Guangxi, this study aimed to determine the effects of sugarcane leaf return (SLR) and fertilizer reduction (FR) on maize growth, yield component and yield, and soil properties. A pot experiment with three SLR amounts (full SLR (FS), 120 g/pot; half SLR (HS), 60 g/pot; and no SLR (NS) with three FR levels including full fertilizer (FF), 4.50 g N/pot, 3.00 g P2O5/pot, and 4.50 g K2O/pot; half fertilizer (HF), 2.25 g N/pot, 1.50 g P2O5/pot, and 2.25 g K2O/pot; and no fertilizer (NF)), without nitrogen, phosphorous, and potassium added, was conducted to assess the effects of different SLR amounts and chemical FR levels on maize growth, yield, and soil properties. Compared with no sugarcane leaf return and the no-fertilizer treatment (CK), SLR and FR could increase maize plant height, stalk diameter, number of fully developed maize plant leaves, total leaf area and chlorophyll content, soil alkali–hydrolyzable nitrogen (AN), available phosphorus (AP), available potassium (AK), soil organic matter (SOM), and electrical conductivity (EC). The maize yield component factors of FS and HS were higher in NF treatment than those in NS treatment. The relative increase rate of treatments retained FF/NF and HF/NF under FS or HS condition on 1000 kernel weight, ear diameter, plant air-dried weight, ear height, and yield than that under NS condition. FSHF had not only the largest plant air-dried weight but also the highest maize yield (3225.08 kg/hm2) among nine treatment combinations. The effects of SLR on maize growth and yield and soil properties were lower than those of FR. SLR and FR combined treatment did not affect maize growth but affected maize yield significantly. Soil properties improved more with SLR + FR treatment than with SLR or FR application alone. The plant height, stalk diameter, number of fully developed maize plant leaves, and total leaf area, as well as AN, AP, AK, SOM, and EC levels in soil, were enhanced by SLR and FR incorporation. The experimental results indicated that applying reasonable FR combined with SLR increased AN, AP, AK, SOM, and EC, which improved maize growth and yield and enhanced soil properties in red soil. Hence, FSHF might be a suitable combination of SLR and FR.