bluuebunny/crossref_metadata_2025 huggingface dataset:
Astragaloside IV Inhibits Galactose-Deficient IgA1 Secretion via miR-98-5p in Pediatric IgA Nephropathy
Authors: Liu Caiqiong , Li Xiaoyan , Shuai Lanjun , Dang Xiqiang , Peng Fangrong , Zhao Mingyi , Xiong Shiqiu , Liu Ying , He Qingnan
Published: April 2021

Purpose: The factor associated with IgA nephropathy (IgAN) is an abnormality of IgA known as galactose-deficient IgA1 (Gd-IgA1). The purpose of this study was to determine the molecular role played by miRNAs in the formation of Gd-IgA1 in IgAN and investigate the regulatory role of Astragaloside IV (AS-IV) in miRNAs.

Patients and methods: Bioinformatics analysis, along with functional and mechanistic experiments, were used to investigate the relationship and function of miRNA, β-1, 3-galactosyltransferase (C1GALT1), Gd-IgA1, and AS-IV. Analyses involved a series of tools, including quantitative real-time polymerase chain reaction (qRT-qPCR), Western blot, enzyme-linked immunosorbent assay (ELISA), Vicia Villosa lectin-binding assay (VVA), Cell counting kit-8 assay (CCK-8), and the dual-luciferase reporter assay.

Results: miRNA screening and validation showed that miR-98-5p was significantly upregulated in the peripheral blood mononuclear cells (PBMCs) of pediatric patients with IgAN compared with patients diagnosed with mesangial proliferative glomerulonephritis (MsPGN) and immunoglobulin A vasculitis nephritis (IgAV-N), and healthy controls (p < 0.05). Experiments with the dual-luciferase reporter confirmed that miR-98-5p might target C1GALT1. The overexpression of miR-98-5p in DAKIKI cells decreased both the mRNA and protein levels of C1GALT1 and increased the levels of Gd-IgA1 levels; these effects were reversed by co-transfection with the C1GALT1 plasmid, and vice versa. In addition, AS-IV downregulated the levels of Gd-IgA1 level in DAKIKI cells by inhibiting miR-98-5p.

Conclusions: Our results revealed that AS-IV could inhibit Gd-IgA1 secretion via miR-98-5p. Increased levels of miR-98-5p in pediatric IgAN patients might affect the glycosylation of IgA1 by targeting C1GALT1. In addition, our analyses suggest that the pathogenesis of IgAN may differ from that of IgAV-N. Collectively, these results provide significant insight into the pathogenesis of IgAN and identify a potential therapeutic target.
Pharmacological Activities and Mechanisms of Hirudin and Its Derivatives - A Review
Authors: Junren Chen , Xiaofang Xie , Huiqiong Zhang , Gangmin Li , Yanpeng Yin , Xiaoyu Cao , Yuqing Gao , Yanan Li , Yue Zhang , Fu Peng , Cheng Peng
Published: April 2021

Hirudin, an acidic polypeptide secreted by the salivary glands of Hirudo medicinalis (also known as “Shuizhi” in traditional Chinese medicine), is the strongest natural specific inhibitor of thrombin found so far. Hirudin has been demonstrated to possess potent anti-thrombotic effect in previous studies. Recently, increasing researches have focused on the anti-thrombotic activity of the derivatives of hirudin, mainly because these derivatives have stronger antithrombotic activity and lower bleeding risk. Additionally, various bioactivities of hirudin have been reported as well, including wound repair effect, anti-fibrosis effect, effect on diabetic complications, anti-tumor effect, anti-hyperuricemia effect, effect on cerebral hemorrhage, and others. Therefore, by collecting and summarizing publications from the recent two decades, the pharmacological activities, pharmacokinetics, novel preparations and derivatives, as well as toxicity of hirudin were systematically reviewed in this paper. In addition, the clinical application, the underlying mechanisms of pharmacological effects, the dose-effect relationship, and the development potential in new drug research of hirudin were discussed on the purpose of providing new ideas for application of hirudin in treating related diseases.
High-Fat Breakfast Increases Bioavailability of Albendazole Compared to Low-Fat Breakfast: Single-Dose Study in Healthy Subjects
Authors: Ochoa Dolores , Saiz-Rodríguez Miriam , González-Rojano Esperanza , Román Manuel , Sánchez-Rojas Sergio , Wojnicz Aneta , Ruiz-Nuño Ana , García-Arieta Alfredo , Abad-Santos Francisco
Published: April 2021

Purpose: Albendazole is a benzimidazole carbamate drug with anthelmintic and antiprotozoal activity against intestinal and tissue parasites. It has been described that the administration with meals increases albendazole absorption. Our aim was to compare the systemic exposure in healthy volunteers of two albendazole formulations after a single oral dose under fed conditions and to evaluate the effect of breakfast composition on albendazole and albendazole sulfoxide bioavailability.

Methods: 12 healthy volunteers were included in a 4-period, 4-sequence, crossover, open, randomized, bioequivalence clinical trial, including two stages to compare two formulations of albendazole. Single oral doses of 400 mg albendazole were administered under fed conditions (a low-fat breakfast in first stage and a high-fat breakfast in the second) separated by 7-day washout periods. Plasma albendazole and albendazole sulfoxide concentrations were measured by HPLC-MS/MS.

Findings: Albendazole absorption was clearly influenced by the meal composition. A high-fat breakfast increased albendazole and albendazole sulfoxide area under the concentration–time curve (AUC) and maximum concentration (Cmax) by double, compared to a low-fat breakfast. The bioavailability of the two formulations was very similar, although the sample size was not sufficient to demonstrate bioequivalence because the intraindividual variability of albendazole was approximately 60%.

Implications: The higher albendazole and albendazole sulfoxide levels when administered with a high-fat meal could be of importance in clinical practice. Since albendazole labeling recommends its administration with meals, it is necessary to insist on taking it with a fatty meal so that the effectiveness of albendazole is not compromised.
Microrheology With an Anisotropic Optical Trap
Authors: Matheson Andrew B. , Mendonca Tania , Gibson Graham M. , Dalgarno Paul A. , Wright Amanda J. , Paterson Lynn , Tassieri Manlio
Published: April 2021

Microrheology with optical tweezers (MOT) measurements are usually performed using optical traps that are close to isotropic across the plane being imaged, but little is known about what happens when this is not the case. In this work, we investigate the effect of anisotropic optical traps on microrheology measurements. This is an interesting problem from a fundamental physics perspective, but it also has practical ramifications because in 3D all optical traps are anisotropic due to the difference in the intensity distribution of the trapping laser along axes parallel and perpendicular to the direction of beam propagation. We find that attempting viscosity measurements with highly anisotropic optical traps will return spurious results, unless the axis with maximum variance in bead position is identified. However, for anisotropic traps with two axes of symmetry such as traps with an elliptical cross section, the analytical approach introduced in this work allows us to explore a wider range of time scales than those accessible with symmetric traps. We have also identified a threshold level of anisotropy in optical trap strength of ~30%, below which conventional methods using a single arbitrary axis can still be used to extract valuable microrheological results. We envisage that the outcomes of this study will have important practical ramifications on how all MOT measurements should be conducted and analyzed in future applications.
Diamond Detectors for Radiotherapy X-Ray Small Beam Dosimetry
Authors: Talamonti C. , Kanxheri K. , Pallotta S. , Servoli L.
Published: April 2021

Many new X-Ray treatment machines using small and/or non-standard radiation fields, e.g., Tomotherapy, Cyber-knife, and linear accelerators equipped with high-resolution multi-leaf collimators and on-board imaging system, have been introduced in the radiotherapy clinical routine within the last few years. The introduction of these new treatment modalities has led to the development of high conformal radiotherapy treatment techniques like Intensity Modulated photon Radiation Therapy, Volumetric Modulated Arc Therapy, and stereotactic radiotherapy. When using these treatment techniques, patients are exposed to non-uniform radiation fields, high dose gradients, time and space variation of dose rates, and beam energy spectrum. This makes reaching the required degree of accuracy in clinical dosimetry even more demanding. Continuing to use standard field procedures and detectors in fields smaller than 3 × 3 cm2, will generate a reduced accuracy of clinical dosimetry, running the risk to overshadowing the progress made so far in radiotherapy applications. These dosimetric issues represent a new challenge for medical physicists. To choose the most appropriate detector for small field dosimetry, different features must be considered. Short- and long-term stability, linear response to the absorbed dose and dose rate, no energy and angular dependence, are all needed but not sufficient. The two most sought-after attributes for small field dosimetry are water equivalence and small highly sensitive (high sensitivity) volumes. Both these requirements aim at minimizing perturbations of charged particle fluence approaching the Charged Particle Equilibrium condition as much as possible, while maintaining high spatial resolution by reducing the averaging effect for non-uniform radiation fields. A compromise between different features is necessary because no dosimeter currently fulfills all requirements, but diamond properties seem promising and could lead to a marked improvement. Diamonds have long been used as materials for dosimeters, but natural diamonds were only first used for medical applications in the 80 s. The availability of reproducible synthetic diamonds at a lower cost compared to natural ones made the diffusion of diamonds in dosimetry possible. This paper aims to review the use of synthetic poly and single-crystal diamond dosimeters in radiotherapy, focusing on their performance under MegaVoltage photon beams. Both commercial and prototype diamond dosimeters behaviour are described and analyzed. Moreover, this paper will report the main related results in literature, considering diamond development issues like growth modalities, electrical contacts, packaging, readout electronics, and how do they affect all the dosimetric parameters of interest such as signal linearity, energy dependence, dose-rate dependence, reproducibility, rise and decay times.
Heterodyne Optical Time Domain Reflectometer Combined With Active Loss Compensation: A Practical Tool for Investigating Fermi Pasta Ulam Recurrence Process and Breathers Dynamics in Optical Fibers
Authors: Naveau Corentin , Vanderhaegen Guillaume , Szriftgiser Pascal , Martinelli Gilbert , Droques Maxime , Kudlinski Alexandre , Conforti Matteo , Trillo Stefano , Akhmediev Nail , Mussot Arnaud
Published: April 2021

We report recent results obtained with a novel optical fiber experimental setup based on a heterodyne optical time-domain reflectometer in the context of FPU recurrence process. Moreover, we actively compensate the dissipation of the system. We show that we can observe several FPU recurrences by monitoring the power and relative phase evolutions of the main discrete frequency components involved in the process.
Comparison of Short-Read Sequence Aligners Indicates Strengths and Weaknesses for Biologists to Consider
Authors: Musich Ryan , Cadle-Davidson Lance , Osier Michael V.
Published: April 2021

Aligning short-read sequences is the foundational step to most genomic and transcriptomic analyses, but not all tools perform equally, and choosing among the growing body of available tools can be daunting. Here, in order to increase awareness in the research community, we discuss the merits of common algorithms and programs in a way that should be approachable to biologists with limited experience in bioinformatics. We will only in passing consider the effects of data cleanup, a precursor analysis to most alignment tools, and no consideration will be given to downstream processing of the aligned fragments. To compare aligners [Bowtie2, Burrows Wheeler Aligner (BWA), HISAT2, MUMmer4, STAR, and TopHat2], an RNA-seq dataset was used containing data from 48 geographically distinct samples of the grapevine powdery mildew fungus Erysiphe necator. Based on alignment rate and gene coverage, all aligners performed well with the exception of TopHat2, which HISAT2 superseded. BWA perhaps had the best performance in these metrics, except for longer transcripts (>500 bp) for which HISAT2 and STAR performed well. HISAT2 was ~3-fold faster than the next fastest aligner in runtime, which we consider a secondary factor in most alignments. At the end, this direct comparison of commonly used aligners illustrates key considerations when choosing which tool to use for the specific sequencing data and objectives. No single tool meets all needs for every user, and there are many quality aligners available.
A Pilot Approach Investigating the Potential of Crop Rotation With Sainfoin to Reduce Meloidogyne enterolobii Infection of Maize Under Greenhouse Conditions
Authors: Rashidifard Milad , Ashrafi Samad , Claassens Sarina , Thünen Torsten , Fourie Hendrika
Published: April 2021

Root-knot nematodes (RKNs) are one of the most important plant-parasitic nematodes of cereal crops in sub-Saharan Africa. This study was designed to evaluate the rotation effects of different cultivars of sainfoin (Esparsette, Perly, Taja and Visnovsky), soybean (DM-5953-RSF) and alfalfa (BAR 7) with maize (P-2432-R), on a Meloidogyne enterolobii population, compared to monoculture maize. The results showed that sainfoin (Perly and Esparsette) and alfalfa had significantly (P ≤ 0.05) lower numbers of M. enterolobii eggs and second stage juveniles (J2) compared to the monoculture maize in the first experiment. However, in the repeat experiment all treatments had significantly (P ≤ 0.05) lower numbers of eggs and J2 compared to monoculture maize. Rotation of sainfoin Esparsette/maize resulted in the lowest numbers of eggs and J2 (91 and 202, respectively) in the first and repeat experiments. Rotation of sainfoin Esparsette/maize reduced M. enterolobii population density by 81 and 60% in the first and repeat experiments, respectively, followed by alfalfa (54 and 43%, respectively). Ultimately, substantial variation was evident in terms of the efficacy of different sainfoin cultivars with regards to their effect on nematode reduction when used in rotation with maize.
Vascular Connections Into the Grape Berry: The Link of Structural Investment to Seededness
Authors: Xiao Zeyu , Chin Sabrina , White Rosemary G. , Gourieroux Aude M. , Pagay Vinay , Tyerman Stephen D. , Schmidtke Leigh M. , Rogiers Suzy Y.
Published: April 2021

Vascular bundles in the grape pedicel and berry contain the conduits, phloem and xylem, for transport of water, sugar, nutrients and signals into and through the grape berry and play a critical role in berry growth and composition. Here, we assess the vascular anatomy within the proximal region of the berry. Guided using a 3D berry model generated by micro-CT, differential staining of transverse sections of berries and receptacles was followed by fluorescent microscopy. Morphometric and vascular characteristics were analyzed within the central proximal region (brush zone, a fibrous extension from the pedicel vascular system into the berry) of the seeded cultivars Shiraz and Sauvignon Blanc, as well as the stenospermocarpic cultivars Ruby Seedless and Flame Seedless. Observations revealed a change in vascular arrangement from the receptacle into the berry brush zone and differences in xylem element size as well as xylem and phloem area relationships. Xylem anatomical and derived hydraulic parameters, as well as total tissue area of xylem and phloem varied between cultivars and in receptacle and berry components. Variation in vascular growth between grape pedicels and berries was independent of seededness. Differences in receptacle xylem vessel size and distribution could contribute to cultivar-dependent xylem backflow constraint.
Measurement of the Effects of School Psychological Services: A Scoping Review
Authors: Müller Bettina , von Hagen Alexa , Vannini Natalie , Büttner Gerhard
Published: April 2021

School psychologists are asked to systematically evaluate the effects of their work to ensure quality standards. Given the different types of methods applied to different users of school psychology measuring the effects of school psychological services is a complex task. Thus, the focus of our scoping review was to systematically investigate the state of past research on the measurement of the effects of school psychological services published between 1998 and 2018 in eight major school psychological journals. Of the 5,048 peer-reviewed articles published within this period, 623 were coded by two independent raters as explicitly refering to school psychology or counseling in the school context in their titles or abstracts. However, only 22 included definitions of effects of school psychological services or described outcomes used to evaluate school psychological services based on full text screening. These findings revealed that measurement of the effects of school psychological services has not been a focus of research despite its' relevance in guidelines of school psychological practice.
An Optimal Choice of Cognitive Diagnostic Model for Second Language Listening Comprehension Test
Authors: Dong Yanyun , Ma Xiaomei , Wang Chuang , Gao Xuliang
Published: April 2021

Cognitive diagnostic models (CDMs) show great promise in language assessment for providing rich diagnostic information. The lack of a full understanding of second language (L2) listening subskills made model selection difficult. In search of optimal CDM(s) that could provide a better understanding of L2 listening subskills and facilitate accurate classification, this study carried a two-layer model selection. At the test level, A-CDM, LLM, and R-RUM had an acceptable and comparable model fit, suggesting mixed inter-attribute relationships of L2 listening subskills. At the item level, Mixed-CDMs were selected and confirmed the existence of mixed relationships. Mixed-CDMs had better model and person fit than G-DNIA. In addition to statistical approaches, the content analysis provided theoretical evidence to confirm and amend the item-level CDMs. It was found that semantic completeness pertaining to the attributes and item features may influence the attribute relationships. Inexplicable attribute conflicts could be a signal of suboptimal model choice. Sample size and the number of multi-attribute items should be taken into account in L2 listening cognitive diagnostic modeling studies. This study provides useful insights into the model selection and the underlying cognitive process for L2 listening tests.
An Approach to Aligning Categorical and Continuous Time Series for Studying the Dynamics of Complex Human Behavior
Authors: Kodama Kentaro , Shimizu Daichi , Dale Rick , Sekine Kazuki
Published: April 2021

An emerging perspective on human cognition and performance sees it as a kind of self-organizing phenomenon involving dynamic coordination across the body, brain and environment. Measuring this coordination faces a major challenge. Time series obtained from such cognitive, behavioral, and physiological coordination are often complicated in terms of non-stationarity and non-linearity, and in terms of continuous vs. categorical scales. Researchers have proposed several analytical tools and frameworks. One method designed to overcome these complexities is recurrence quantification analysis, developed in the study of non-linear dynamics. It has been applied in various domains, including linguistic (categorical) data or motion (continuous) data. However, most previous studies have applied recurrence methods individually to categorical or continuous data. To understand how complex coordination works, an integration of these types of behavior is needed. We aimed to integrate these methods to investigate the relationship between language (categorical) and motion (continuous) directly. To do so, we added temporal information (a time stamp) to categorical data (i.e., language), and applied joint recurrence analysis methods to visualize and quantify speech-motion coordination coupling during a rap performance. We illustrate how new dynamic methods may capture this coordination in a small case-study design on this expert rap performance. We describe a case study suggesting this kind of dynamic analysis holds promise, and end by discussing the theoretical implications of studying complex performances of this kind as a dynamic, coordinated phenomenon.
A Diary Study on When and With Whom Recovery Experiences Modulate Daily Stress and Worry During a COVID-19 Lockdown
Authors: Ménard Julie , Foucreault Annie , Leduc Hugues , Meunier Sophie , Trépanier Sarah-Geneviève
Published: April 2021

In April 2020, almost six out of 10 people around the world were in lockdown due to the COVID-19 pandemic. Being locked down usually has a deleterious effect on the confined individual's mental health. In this exceptionally challenging context, finding ways to minimize negative mood about the pandemic is essential. Pandemic-related negative states (“negative mood”) and recovery experiences were investigated in a sample of 264 individuals who completed daily surveys four times per day over 7 consecutive days. MSEMs analyses revealed that negative mood persisted from moment-to-moment through the day, thus showing a response lag effect. Further analyses revealed that when someone experienced pandemic-related psychological detachment, relaxation, mastery, control, pleasure, or relatedness at specific periods of the day, mood had improved at the next measured time period, suggesting a protective effect. However, the pattern displayed by singles with dependents suggests that some recovery experiences at specific periods during the day seem to have a backfiring effect and worsen subsequent mood. These findings bring new insight into the role of recovery experiences during lockdowns and suggest that many could benefit from such experiences throughout the day when self-isolating. However, for individuals with multiple risk factors such as being single with dependents, some recovery experiences, at specific periods during the day, might not bring the desired outcome and future research is needed to examine if guilt or domestic burden may explain this finding. Results contribute to our understanding of how to take care of one's mental health during the current pandemic, and concrete recommendations adapted to individual contexts are provided.
How Do University Students’ Perceptions of the Instructor’s Role Influence Their Learning Outcomes and Satisfaction in Cloud-Based Virtual Classrooms During the COVID-19 Pandemic?
Authors: Wang Rong , Han Jiying , Liu Chuanyong , Xu Hongji
Published: April 2021

This study examined the relationships between the role of the instructor and university students’ learning outcomes in cloud-based classrooms during the COVID-19 (coronavirus disease 2019) pandemic. The results of an online survey of 7,210 university students in mainland China revealed that the students’ perceived learning outcomes and learning satisfaction were positively related to instructor innovation and negatively related to instructor performance. Instructional support was positively related to the students’ perceived learning outcomes but not directly related to their learning satisfaction. The students’ academic self-efficacy mediated the influence of instructional support and instructor innovation on their perceived learning outcomes and learning satisfaction. The results contribute to knowledge of the role instructors play in facilitating students’ learning outcomes in higher education and suggest ways to improve the learning environment and learning outcomes, especially in cloud-based virtual classrooms.
Problematic Pornography Use in Japan: A Preliminary Study Among University Students
Authors: Okabe Yushun , Takahashi Fumito , Ito Daisuke
Published: April 2021

Background

Problematic pornography use is considered an addictive behavior, which is an important clinical issue. Despite considerable research interest in problematic pornography use worldwide, to the best of our knowledge, there are no extant studies on the subject in Japan. Therefore, despite the fact that many people in Japan use pornography, the difference between problematic and non-problematic users among Japanese people is not known.

Objective

This study aimed to identify the characteristics of problematic pornography use among Japanese students, to the best of our knowledge. Specifically, we examined general psychopathological symptoms, sexual compulsivity, depression, anxiety, and low effortful control.

Methods

The participants were 150 college students aged 20–26 years (mean age = 21.5, SD = 1.21, males: n = 86, females: n = 64) at a university in midland Japan. An online questionnaire was administered that included items on pornography usage patterns, impaired control of pornography use, sexual compulsivity, depression, anxiety, and effortful control.

Results

Most men (97%) and approximately one-third of women (35.9%) used pornography at least once in the past month. Some users reported significant daily-life problems due to difficulty in controlling pornography use (5.7%). Participants with impaired control of pornography use had higher depression, anxiety, and sexual compulsivity, and lower effortful control than pornography users without impaired control.

Conclusion

Some Japanese students reported significant daily-life problems due to impaired control of pornography use. The characteristics of individuals with impaired control are consistent with previous studies. The results of this study suggest that individuals with impaired control may have poor mental health, and that there is need for further research and development of treatment systems to manage this issue in Japan. Further research exploring a more varied sample in Japan is required to effectively examine problematic pornography use.
Beliefs in Conspiracy Theories and Misinformation About COVID-19: Comparative Perspectives on the Role of Anxiety, Depression and Exposure to and Trust in Information Sources
Authors: De Coninck David , Frissen Thomas , Matthijs Koen , d’Haenens Leen , Lits Grégoire , Champagne-Poirier Olivier , Carignan Marie-Eve , David Marc D. , Pignard-Cheynel Nathalie , Salerno Sébastien , Généreux Melissa
Published: April 2021

While COVID-19 spreads aggressively and rapidly across the globe, many societies have also witnessed the spread of other viral phenomena like misinformation, conspiracy theories, and general mass suspicions about what is really going on. This study investigates how exposure to and trust in information sources, and anxiety and depression, are associated with conspiracy and misinformation beliefs in eight countries/regions (Belgium, Canada, England, Philippines, Hong Kong, New Zealand, United States, Switzerland) during the COVID-19 pandemic. Data were collected in an online survey fielded from May 29, 2020 to June 12, 2020, resulting in a multinational representative sample of 8,806 adult respondents. Results indicate that greater exposure to traditional media (television, radio, newspapers) is associated with lower conspiracy and misinformation beliefs, while exposure to politicians and digital media and personal contacts are associated with greater conspiracy and misinformation beliefs. Exposure to health experts is associated with lower conspiracy beliefs only. Higher feelings of depression are also associated with greater conspiracy and misinformation beliefs. We also found relevant group- and country differences. We discuss the implications of these results.
The Role of Food in the Family Relationships of Adolescents With Anorexia Nervosa and Bulimia in Northeastern Brazil: A Qualitative Study Using Photo Elicitation
Authors: Ramalho Juniana de Almeida Mota , El Husseini Mayssa' , Bloc Lucas , Bucher-Maluschke Julia Sursis Nobre Ferro , Moro Marie Rose , Lachal Jonathan
Published: April 2021

Family components can play roles both as protective factors and maintenance mechanisms of eating disorders. We aimed to investigate the role of food in the family relationships of adolescents with anorexia nervosa and bulimia in northeastern Brazil. Using photo elicitation, a visual narrative method that gives insight into the participants' perspective through photograph, we conducted semi-structured interviews with 26 people: four teenage girls with anorexia, four with bulimia, eight mothers, four fathers, five grandmothers, and one sister. Data were analyzed using the principles of Interpretative Phenomenological Analysis, which highlighted the following themes: control of the parent-adolescent relationship through food; food as a mean of experiencing parental presence-absence; food as the focus of conflict in the nuclear family, and food as a source of three-generational conflict. Food seemed to be a means for teens and parents to express physical suffering and psychological violence. Moreover, mourning appeared to influence the girls' relationships with food. Conflict in these families is not focused solely on food, but extends to other subjects, and teenagers' emotional reactivity concerning their relationship with their parents and food during family mealtimes varied. These features reinforced the cultural aspect and influences of eating experiences among adolescent girls with eating disorders. Remarkable disparities exist in the generations' views on what rules and rituals these adolescents must follow at meals. These disparities can obfuscate generational boundaries in these families. Our data reinforce the need to focus on the adolescent's autonomy in the family setting and on family identity as related to food among three generations. These findings necessitate a reorganization of boundaries between these generations.
The Utility of ICD-11 and DSM-5 Traits for Differentiating Patients With Personality Disorders From Other Clinical Groups
Authors: Pires Rute , Henriques-Calado Joana , Sousa Ferreira Ana , Bach Bo , Paulino Marco , Gama Marques João , Ribeiro Moreira Ana , Grácio Jaime , Gonçalves Bruno
Published: April 2021

The ICD-11 Classification of Personality Disorders delineates five trait domain qualifiers (i.e., negative affectivity, detachment, dissociality, disinhibition, and anankastia), whereas the DSM-5 Alternative Model of Personality Disorders also delineates a separate domain of psychoticism. These six combined traits not only characterize individual stylistic features, but also the severity of their maladaptive expressions. It was, therefore, the aim of this study to investigate the utility of ICD-11 and DSM-5 trait domains to differentiate patients with personality disorders (PD) from patients with other mental disorders (non-PD). The Personality Inventory for DSM-5 Brief Form Plus (PID5BF+M) was administered to a sample of patients diagnosed with a personality disorder (N = 124, Mage = 42.21, 42.7% females) along with a sample of patients diagnosed with other mental disorders (N = 335, Mage = 44.83, 46.6% females). Group differences were explored using the independent sample t test or the Mann–Whitney U test for independent samples, and discriminant factor analysis was used to maximize group differences for each trait domain and facet score. The PD group showed significantly higher scores for the total PID5BF+M composite score, for the trait domains of negative affectivity, antagonism/dissociality, and disinhibition and for the trait facets of emotional lability, manipulativeness, deceitfulness, and impulsivity. The trait domains of disinhibition, negative affectivity, and antagonism/dissociality as well as the trait facets of impulsivity, deceitfulness, emotional lability, and manipulativeness were the best discriminators between PD and non-PD patients. The global PID5BF+M composite score was also one of the best discriminators supporting its potential as a global severity index for detecting personality dysfunction. Finally, high scores in three or more of the 18 PID5BF+M facets suggested the possible presence of a PD diagnosis. Despite some limitations, our findings suggest that the ICD-11 and DSM-5 traits have the potential to specifically describe the stylistic features that characterize individuals with PD, including the severity of their maladaptive expressions.
Effective Adoption of Tablets for Psychodiagnostic Assessments in Rural Burundi: Evidence for the Usability and Validity of Mobile Technology in the Example of Differentiating Symptom Profiles in AMISOM Soldiers 1 Year After Deployment
Authors: Weierstall Roland , Crombach Anselm , Nandi Corina , Bambonyé Manassé , Probst Thomas , Pryss Rüdiger
Published: April 2021

Research on the use of mobile technology in health sciences has identified several advantages of so-called mHealth (mobile health) applications. Tablet-supported clinical assessments are becoming more and more prominent in clinical applications, even in low-income countries. The present study used tablet computers for assessments of clinical symptom profiles in a sample of Burundian AMISOM soldiers (i.e., African Union Mission to Somalia; a mission approved by the UN). The study aimed to demonstrate the feasibility of mHealth-supported assessments in field research in Burundi. The study was conducted in a resource-poor setting, in which tablet computers are predestined to gather data in an efficient and reliable manner. The overall goal was to prove the validity of the obtained data as well as the feasibility of the chosen study setting. Four hundred sixty-three soldiers of the AMISOM forces were investigated after return from a 1-year military mission in Somalia. Symptoms of posttraumatic stress disorder (PTSD) and depression were assessed. The used data-driven approach based on a latent profile analysis revealed the following four distinct groups, which are based on the soldiers' PTSD and depression symptom profiles: Class 1: moderate PTSD, Class 2: moderate depression, Class 3: low overall symptoms, and Class 4: high overall symptoms. Overall, the four identified classes of soldiers differed significantly in their PTSD and depression scores. The study clearly demonstrates that tablet-supported assessments can provide a useful application of mobile technology in large-scale studies, especially in resource-poor settings. Based on the data collected for the study at hand, it was possible to differentiate different sub-groups of soldiers with distinct symptom profiles, proving the statistical validity of the gathered data. Finally, advantages and challenges for the application of mobile technology in a resource-poor setting are outlined and discussed.
Motion Smoothness Metrics for Cannulation Skill Assessment: What Factors Matter?
Authors: Singh Simar , Bible Joe , Liu Zhanhe , Zhang Ziyang , Singapogu Ravikiran
Published: April 2021

Medical training simulators have the potential to provide remote and automated assessment of skill vital for medical training. Consequently, there is a need to develop “smart” training devices with robust metrics that can quantify clinical skills for effective training and self-assessment. Recently, metrics that quantify motion smoothness such as log dimensionless jerk (LDLJ) and spectral arc length (SPARC) are increasingly being applied in medical simulators. However, two key questions remain about the efficacy of such metrics: how do these metrics relate to clinical skill, and how to best compute these metrics from sensor data and relate them with similar metrics? This study addresses these questions in the context of hemodialysis cannulation by enrolling 52 clinicians who performed cannulation in a simulated arteriovenous (AV) fistula. For clinical skill, results demonstrate that the objective outcome metric flash ratio (FR), developed to measure the quality of task completion, outperformed traditional skill indicator metrics (years of experience and global rating sheet scores). For computing motion smoothness metrics for skill assessment, we observed that the lowest amount of smoothing could result in unreliable metrics. Furthermore, the relative efficacy of motion smoothness metrics when compared with other process metrics in correlating with skill was similar for FR, the most accurate measure of skill. These results provide guidance for the computation and use of motion-based metrics for clinical skill assessment, including utilizing objective outcome metrics as ideal measures for quantifying skill.
Towards a Machine Vision-Based Yield Monitor for the Counting and Quality Mapping of Shallots
Authors: Boatswain Jacques Amanda A. , Adamchuk Viacheslav I. , Park Jaesung , Cloutier Guillaume , Clark James J. , Miller Connor
Published: April 2021

In comparison to field crops such as cereals, cotton, hay and grain, specialty crops often require more resources, are usually more sensitive to sudden changes in growth conditions and are known to produce higher value products. Providing quality and quantity assessment of specialty crops during harvesting is crucial for securing higher returns and improving management practices. Technical advancements in computer and machine vision have improved the detection, quality assessment and yield estimation processes for various fruit crops, but similar methods capable of exporting a detailed yield map for vegetable crops have yet to be fully developed. A machine vision-based yield monitor was designed to perform size categorization and continuous counting of shallots in-situ during the harvesting process. Coupled with a software developed in Python, the system is composed of a video logger and a global navigation satellite system. Computer vision analysis is performed within the tractor while an RGB camera collects real-time video data of the crops under natural sunlight conditions. Vegetables are first segmented using Watershed segmentation, detected on the conveyor, and then classified by size. The system detected shallots in a subsample of the dataset with a precision of 76%. The software was also evaluated on its ability to classify the shallots into three size categories. The best performance was achieved in the large class (73%), followed by the small class (59%) and medium class (44%). Based on these results, the occasional occlusion of vegetables and inconsistent lighting conditions were the main factors that hindered performance. Although further enhancements are envisioned for the prototype system, its modular and novel design permits the mapping of a selection of other horticultural crops. Moreover, it has the potential to benefit many producers of small vegetable crops by providing them with useful harvest information in real-time.
XRSISE: An XR Training System for Interactive Simulation and Ergonomics Assessment
Authors: Pavlou Michail , Laskos Dimitrios , Zacharaki Evangelia I. , Risvas Konstantinos , Moustakas Konstantinos
Published: April 2021

The use of virtual reality (VR) techniques for industrial training provides a safe and cost effective solution that contributes to increased engagement and knowledge retention levels. However, the process of experiential learning in a virtual world without biophysical constraints might contribute to muscle strain and discomfort, if ergonomic risk factors are not considered in advance. Under this scope, we have developed a digital platform which employs extended reality (XR) technologies for the creation and delivery of industrial training programs, by taking into account the users and workplace specificities through the adaptation of the 3D virtual world to the real environment. Our conceptual framework is composed of several inter-related modules: 1) the XR tutorial creation module, for automatic recognition of the sequence of actions composing a complex scenario while this is demonstrated by the educator in VR, 2) the XR tutorial execution module, for the delivery of visually guided and personalized XR training experiences, 3) the digital human model (DHM) based simulation module for creation and demonstration of job task simulations avoiding the need of an actual user and 4) the biophysics assessment module for ergonomics analysis given the input received from the other modules. Three-dimensional reconstruction and aligned projection of the objects situated in the real scene facilitated the imposition of inherent physical constraints, thereby allowed to seamlessly blend the virtual with the real world without losing the sense of presence.
The roAp Stars Observed by the Kepler Space Telescope
Authors: Holdsworth Daniel L.
Published: April 2021

Before the launch of theKeplerSpace Telescope, most studies of the rapidly oscillating Ap (roAp) stars were conducted with ground-based photometricBobservations, supplemented with high-resolution time-resolved spectroscopy and some space observations with the WIRE, MOST, and BRITE satellites. These modes of observation often only provided information on a single star at a time, however,Keplerprovided the opportunity to observe hundreds of thousands of stars simultaneously. Over the duration of the primary 4 yearKeplermission, and its 4 year reconfigured K2 mission, the telescope observed at least 14 new and known roAp stars. This paper provides a summary the results of these observations, including a first look at the entire data sets, and provides a look forward to NASA'sTESSmission.
Circumbinary Habitable Zones in the Presence of a Giant Planet
Authors: Georgakarakos Nikolaos , Eggl Siegfried , Dobbs-Dixon Ian
Published: April 2021

Determining habitable zones in binary star systems can be a challenging task due to the combination of perturbed planetary orbits and varying stellar irradiation conditions. The concept of “dynamically informed habitable zones” allows us, nevertheless, to make predictions on where to look for habitable worlds in such complex environments. Dynamically informed habitable zones have been used in the past to investigate the habitability of circumstellar planets in binary systems and Earth-like analogs in systems with giant planets. Here, we extend the concept to potentially habitable worlds on circumbinary orbits. We show that habitable zone borders can be found analytically even when another giant planet is present in the system. By applying this methodology to Kepler-16, Kepler-34, Kepler-35, Kepler-38, Kepler-64, Kepler-413, Kepler-453, Kepler-1647, and Kepler-1661 we demonstrate that the presence of the known giant planets in the majority of those systems does not preclude the existence of potentially habitable worlds. Among the investigated systems Kepler-35, Kepler-38, and Kepler-64 currently seem to offer the most benign environment. In contrast, Kepler-16 and Kepler-1647 are unlikely to host habitable worlds.
Physical Demands of Women's Soccer Matches: A Perspective Across the Developmental Spectrum
Authors: Vescovi Jason D. , Fernandes Elton , Klas Alexander
Published: April 2021

Female soccer players are exposed to specific physical demands during matches, which vary according to the standard of play. Existing studies have largely focused on quantifying the distances covered for professional and international level players. This approach is limited in scope regarding the broader aspects around physical demands and is detached from development pathway models. An understanding of the demands across all standards will provide valuable insights about appropriate player development and help ensure physical readiness for the demands of the sport. The aim of this perspective paper is to describe the physical demands experienced during women's soccer matches across the developmental spectrum. A combination of evidence from the literature and data from the author's research (JDV) is presented. Specifically highlighted are the trends for locomotor distances, acceleration and deceleration frequency, and metabolic power metrics for youth (≤U17), college (NCAA/U20), professional (domestic) and international standards of women's soccer. In addition, the changes in match demands between levels of play are used to help illustrate gaps that must be overcome in order to successfully achieve physical readiness to compete at higher levels. The evidence demonstrates the importance of training appropriate attributes to prepare female soccer players who are striving to play at progressively higher standards.
Growth-Promoting Potential of Rhizobacteria Isolated From Sugarcane
Authors: Santos Roberta Mendes dos , Rigobelo Everlon Cid
Published: April 2021

The search for plant growth-promoting rhizobacteria (PGPRs) addresses the ongoing need for new bioinoculants to be used on various agricultural crop species, including sugarcane. Bacterial strains were isolated from the rhizosphere of sugarcane plants and identified by sequencing the 16S ribosomal gene. The main indole acetic acid producers wereEnterobactersp. IP11,Enterobactersp. IP14, andE. asburiaeIP24.Achromobacter spaniusIP23 presented the highest levels of cellulolytic activity and potassium solubilization.Bacillus thuringiensisIP21 andStaphylococcus saprophyticusIJ8 showed the highest levels of fixed nitrogen. The levels of calcium phosphate and aluminum phosphate inB. thuringiensisIP21 were notable, as this strain solubilized 481.00 and 39.33 mg of phosphorus mL−1, respectively; however, for Araxá apatite, the results forB. anthracisIP17 were notable (622.99 mg phosphorus mL−1), while for iron phosphate solubilization,Enterobactersp. IP14, which solubilized 105.66 mg phosphorus mL−1was notable. TheB. thuringiensisIP21 andEnterobactersp. IP11 isolates promoted the growth of the tallest sugarcane plants, inducing increases of 14.1 and 10.4% relative to the control plants, respectively. For shoot dry matter, root dry matter, and total dry matter, plants inoculated withEnterobactersp. IP14,B. anthracisIP17, andA. spaniusIP23 presented higher values than the controls. Furthermore, plants inoculated withB. thuringiensisIP21 presented higher root dry matter and total dry matter values, and those inoculated withEnterobactersp. IP14 also presented higher total dry matter values. These results indicate that bacteria with the potential for use as future inoculants should be investigated since bacteria with plant growth-related characteristics may not impact growth promotion.
Tillage, Mulching and Nitrogen Fertilization Differentially Affects Soil Microbial Biomass, Microbial Populations and Bacterial Diversity in a Maize Cropping System
Authors: Nyamwange Methuselah Mang'erere , Njeru Ezekiel Mugendi , Mucheru-Muna Monicah
Published: April 2021

Determination of biologically active components of the soil organic matter, such as soil microbial biomass carbon (C) and nitrogen (N) can be used as indicators for variations in soil productivity due to changes in soil management. Soil agronomic management practices bring about changes in the physical and chemical properties of the soil, resulting in variations in soil microbial biomass and microbial diversity. The effects of tillage, mulch and inorganic fertilizers on soil microbial biomass C and N, microbial populations and bacterial diversity were determined from the treatment combinations which had been applied for 5 years in Central Kenyan Highlands. The test crop used was maize (Zea mays L.). The study involved conventional and minimum tillage systems, mulching and inorganic fertilizers (120 kg N/ha). Tillage (P < 0.001), mulch (P < 0.001), and fertilizer (P = 0.009) significantly affected soil microbial biomass C and N whereby minimum tillage and mulch increased soil microbial biomass C and N. Interestingly, minimum tillage and mulch recorded the highest bacteria and fungi CFUs compared to conventional tillage and inorganic fertilizers. Only fertilizer and mulch (P < 0.001) had significant effect on actinobacteria CFUs. Amplified ribosomal DNA analysis (ARDRA) showed that the highest genetic distance of 0.611 was recorded between treatments conventional tillage + no mulch + no NPK fertilizer and conventional tillage + no mulch + NPK fertilizer. The results demonstrate that minimum tillage and mulching are attractive soil agronomic management practices since they increase soil microbial biomass and bacterial diversity in agricultural soils.