bluuebunny/crossref_metadata_2025 huggingface dataset:
Genetic associations with neural reward responsivity to food cues in children
Authors: Yeum Dabin , Renier Timothy J. , Carlson Delaina D. , Ballarino Grace A. , Lansigan Reina K. , Meyer Meghan L. , Loos Ruth J. F. , Emond Jennifer A. , Masterson Travis D. , Gilbert-Diamond Diane
Published: September 2024

Objective

To test associations of candidate obesity-related single nucleotide polymorphisms (SNPs) and obesity polygenic risk scores (PRS) with neural reward reactivity to food cues.

Methods

After consuming a pre-load meal, 9–12-year-old children completed a functional magnetic resonance imaging (fMRI) paradigm with exposure to food and non-food commercials. Genetic exposures included FTO rs9939609, MC4R rs571312, and a pediatric-specific obesity PRS. A targeted region-of-interest (ROI) analysis for 7 bilateral reward regions and a whole-brain analysis were conducted. Independent associations between each genetic factor and reward responsivity to food cues in each ROI were evaluated using linear models.

Results

Analyses included 151 children (M = 10.9 years). Each FTO rs9939609 obesity risk allele was related to a higher food-cue-related response in the right lateral hypothalamus after controlling for covariates including the current BMI Z-score (p < 0.01), however, the association did not remain significant after applying the multiple testing correction. MC4R rs571312 and the PRS were not related to heightened food-cue-related reward responsivity in any examined regions. The whole-brain analysis did not identify additional regions of food-cue-related response related to the examined genetic factors.

Conclusion

Children genetically at risk for obesity, as indicated by the FTO genotype, may be predisposed to higher food-cue-related reward responsivity in the lateral hypothalamus in the sated state, which, in turn, could contribute to overconsumption.

Clinical trial registration

https://clinicaltrials.gov/study/NCT03766191, identifier NCT03766191.
Case report: Conjunctival melanoma treated with relatlimab and nivolumab showing remarkable response
Authors: Attrash Mirona , Badran Omar , Shapira Yinon , Bar-Sela Gil
Published: September 2024

Conjunctival melanoma, an uncommon form of ocular melanoma, shares some molecular characteristics with cutaneous melanoma and some with mucosal melanoma. Treatment of cases where it becomes advanced or metastatic raises unique treatment challenges. Nivolumab/relatlimab (Opdualag) recently received FDA approval for metastatic melanoma based on the phase 2/3 RELATIVITY-047 trial, which showed better median progression-free survival (PFS) in the first-line setting without new safety signals. The efficacy of this drug in conjunctival melanoma has not been reported yet.

Case presentation

An 87-year-old woman with a history of mild dementia was admitted to the oncology department with a large, exophytic tumor protruding from her left eye, diagnosed as conjunctival melanoma two years previously. This tumor was secreting a whitish fluid and obstructing her vision. Immunotherapy with Opdualag was started, with a near clinical complete response after the 1st cycle. The patient was treated with only four cycles due to worsening of her dementia.

Conclusion

Nivolumab/relatlimab (Opdualag) is a promising treatment alternative in conjunctival melanoma when surgery is not viable.
Alström syndrome—wide clinical variability within the same variant: a case report and literature review
Authors: Jecan-Toader Diana , Trifa Adrian , Lucian Bogdan , Pop Tudor Lucian , Cainap Simona Sorana
Published: September 2024

Background

Alström disease is a rare disorder caused by various variants in the ALMS1 gene. It is characterised by multiorgan involvement, namely neurosensory deficits, endocrine and metabolic disturbances, cardiomyopathy, and hepatic and renal dysfunction. The disease exhibits marked interindividual variability, both in clinical manifestations and age of onset. Several attempts have been made to establish a relationship between phenotype and genotype, with little success.

Methods

We present the case of an infant who presented with dilated cardiomyopathy, above-average weight and neurosensory deficits, raising the suspicion for Alström syndrome, later confirmed through genetic testing. Moreover, we conducted an extensive literature search to identify all reported cases having the same variant as our patient, in order to evaluate whether specific mutated alleles have a role in determining phenotype-genotype associations.

Results

A 4-month-old female infant with a recent history of bronchiolitis was referred to our centre due to a systolic murmur. In our service, the clinical exam was significant for above-average weight, dyspnea, wheezing and a grade II systolic murmur. Echocardiography revealed dilated cardiomyopathy with severe systolic dysfunction of the left ventricle. Laboratory investigations revealed elevated NT-proBNP and troponin levels, along with positive IgM antibodies for CMV and EBV. Dilated cardiomyopathy attributed to viral myocarditis was suspected. Treatment with ACE inhibitors and diuretics was started, with a favourable response initially. However, after a few months, the patient presented with vertical nystagmus and head bobbing. The ophthalmologic exam revealed cone-rode dystrophy. Considering the constellation of symptoms, Alström syndrome was suspected. Genetic testing revealed a homozygous variant [c.4156dup (p.Thr1386Asnfs*15)] in the ALMS1 gene, confirming the diagnosis.

Conclusion

Our literature review revealed 8 additional cases harbouring the same variant as our patient, five in a heterozygous state, two in a homozygous state and one with only one allele identified. The identified patients presented high heterogeneity of clinical manifestations and age of onset. The heterogeneity persisted even in patients with homozygous variants, suggesting the involvement of factors beyond the specific disease-causing variant in determining disease manifestation. Therefore, genotype-phenotype correlations might not be supported by specific variants.
Airspaces-derived exosomes contain disease-relevant protein signatures in a mouse model of cystic fibrosis (CF)-like mucoinflammatory lung disease
Authors: Mao Yun , Suryawanshi Amol , Patial Sonika , Saini Yogesh
Published: September 2024

Exosomes, membrane-bound extracellular vesicles, ranging from approximately 30–200 nm in diameter, are released by almost all cell types and play critical roles in intercellular communication. In response to the prevailing stress, the exosome-bound protein signatures vary in abundance and composition. To identify the bronchoalveolar lavage fluid (BALF) exosome-bound proteins associated with mucoinflammatory lung disease and to gain insights into their functional implications, we compared BALF exosomes-derived proteins from adult Scnn1b transgenic (Scnn1b-Tg+) and wild type (WT) mice. A total of 3,144 and 3,119 proteins were identified in BALF exosomes from Scnn1b-Tg+ and WT mice, respectively. Using cutoff criteria (Log2 fold-change > 1 and adjusted p-value < 0.05), the comparison of identified proteins revealed 127 and 30 proteins that were significantly upregulated and downregulated, respectively, in Scnn1b-Tg+ versus WT mice. In addition, 52 and 27 proteins were exclusively enriched in Scnn1b-Tg+ and WT mice, respectively. The identified exosome-bound proteins from the homeostatic airspaces of WT mice were mostly relevant to the normal physiological processes. The protein signatures enriched in the BALF exosomes of Scnn1b-Tg+ mice were relevant to macrophage activation and mucoinflammatory processes. Ingenuity pathway analyses revealed that the enriched proteins in Scnn1b-Tg+ mice contributed to the inflammatory responses and antimicrobial defense pathways. Selective proteins including, RETNLA/FIZZ1, LGALS3/Galectin-3, S100A8/MRP8, and CHIL3/YM1 were immunolocalized to specific cell types. The comparative analysis between enriched BALF exosome proteins and previously identified differentially upregulated genes in Scnn1b-Tg+ versus WT mice suggested that the compartment-/cell-specific upregulation in gene expression dictates the enrichment of their respective proteins in the lung airspaces. Taken together, this study demonstrates that the BALF exosome-bound protein signatures reflect disease-relevant disturbances. Our findings suggest that the exosomes carry disease-relevant protein signatures that can be used as a diagnostic as well as predictive biomarkers for mucoinflammatory lung disease.
Health enhancing behaviors in early adolescence: an investigation of nutrition, sleep, physical activity, mindfulness and social connectedness and their association with psychological distress and wellbeing
Authors: Bromley Kassie , Sacks Dashiell D. , Boyes Amanda , Driver Christina , Hermens Daniel F.
Published: September 2024

Introduction

Nutrition, sleep and physical activity are termed the “big three” health enhancing behaviors (HEB) associated with psychological distress and wellbeing. This study sought to understand differential associations between an expanded group of HEB (nutrition, sleep, physical activity, mindfulness, social connectedness) and psychological distress/wellbeing in early adolescents.

Methods

Correlational and regression analyses were conducted in N=103 (51% females) adolescents (12.6 ± 0.3 years of age) recruited from the Longitudinal Adolescent Brain Study.

Results

Higher scores on sleep, social connectedness and mindfulness scales were significantly associated with lower psychological distress scores. While higher scores on social connectedness and mindfulness scales were significantly associated with higher wellbeing scores. When adjusting for sex, nutrition, sleep, social connectedness and mindfulness accounted for a significant proportion of variance in the psychological distress model whereas physical activity and social connectedness accounted for a significant proportion of the variance in the wellbeing model.

Discussions

Overall findings make a strong case for expansion of the “big three” HEB to include mindfulness and social connectedness, especially given social connectedness emerged as the strongest predictor of both psychological distress and wellbeing. In addition, this research suggests that early adolescent nutrition, sleep quality, and mindfulness should be prioritized in efforts to reduce risk of difficulties, and physical activity prioritized as a protective factor for wellbeing in this population. Findings have implications for interventions, emphasizing the importance of addressing HEB factors comprehensively and tailoring strategies to the unique needs of early adolescents to foster positive mental health outcomes.
Non-communicable diseases prevention and control by government ministries in Guyana
Authors: Roberts Karen Rebecca Vanese , Ventura Carla Aparecida Arena , de Sousa Alvaro Francisco Lopes , Mendes Isabel Amélia Costa
Published: September 2024

Background

Countries of the Caribbean Community signed the Declaration of Port of Spain in 2007 with the vision to stop the epidemic of non-communicable diseases (NCDs). The adoption of the Declaration by member states represented a regional effort, challenging governments, the private sector, and civil society to act together. Multisectoral actions in Guyana aimed at achieving this goal are the focus of this article, demonstrating the work of different actors in addressing the burden of NCDs in the country.

Objective

To analyze multisectoral actions developed among five ministries for the implementation of the Declaration of Port of Spain 2007 in Guyana.

Methods

This qualitative study was guided by the Consolidated Criteria for Reporting Qualitative Research (COREQ) checklist and conducted in five ministries (Agriculture, Education, Finance, Health, and Trade) in Georgetown, Guyana. The thematic analysis was guided by a framework consisting of four elements: context (why the policy is needed), content (what the policy is mainly about), process (how the policy was brought forward and implemented), and actors (who participates in and influences the formulation and implementation of the policy). The framework considers how these elements interconnect to shape policy development and implementation processes.

Findings

Data analysis provided the researchers with insights into possible topic areas and codes for consideration during the analysis, hence a deductive approach to data analysis was used. The results highlighted the importance of coordination among government entities, national and international agencies, private actors, industry players, and civil society. Participants did not mention the use of responsibility metrics but cited mechanisms that facilitated collaboration.

Conclusion

The results showed limitations in transforming multisectoral initiatives into intersectoral collaboration to achieve real integration among the different actors involved, considering the actual context and content. Actions could be more effectively implemented with significant outcomes for NCD control in Guyana.
A development and implementation of a preconception counseling program for black women and men in the southeastern United States: a pilot protocol
Authors: Hernandez-Green Natalie , Haiman Madison D. , McDonald Adenike , Rollins Latrice , Farinu Oluyemi T. O. , Clarke Lasha S. , Huebshmann Amy , Fort Meredith P. , Chandler Rasheeta D. , Brocke Phebe , McLaurin-Glass Danette , Harris Erin , Berry Kaala , Suarez Angie , Williams Tiyamika , Franklin Cheryl G.
Published: September 2024

Introduction

Racial/ethnic disparities in maternal mortality rates represent one of the most significant areas of disparities amongst all conventional population perinatal health measures in the U.S. The alarming trends and persistent disparities of outcomes by race/ethnicity and geographic location reinforce the need to focus on ensuring quality and safety of maternity care for all women. Despite complex multilevel factors impacting maternal mortality and morbidity, there are evidence-based interventions that, when facilitated consistently and properly, are known to improve the health of mothers before, during and after pregnancy. The objective of this project is to test implementation of pre-conception counseling with father involvement in community-based settings to improve cardiovascular health outcomes before and during pregnancy in southeastern United States.

Methods and analysis

This study has two components: a comprehensive needs and assets assessment and a small-scale pilot study. We will conduct a community informed needs and assets assessment with our diverse stakeholders to identify opportunities and barriers to preconception counseling as well as develop a stakeholder-informed implementation plan. Next, we will use the implementation plan to pilot preconception counseling with father involvement in community-based settings. Finally, we will critically assess the context, identify potential barriers and facilitators, and iteratively adapt the way preconception counseling can be implemented in diverse settings. Results of this research will support future research focused on identifying barriers and opportunities for scalable and sustainable public health approaches to implementing evidence-based strategies that reduce maternal morbidity and mortality in the southeastern United States’ vulnerable communities.

Discussion

Findings will demonstrate that preconception counseling can be implemented in community health settings in the southeastern United States. Furthermore, this study will build the capacity of community-based organizations in addressing the preconception health of their clients. We plan for this pilot to inform a larger scaled-up clinical trial across community health settings in multiple southeastern states.
Beyond The Wi-Fi Era
Authors: Amin Osama , Dang Shuping , Abdelhady Amr M. , Ma Guoqing , Ye Jia , Alouini Mohamed-Slim , Shihada Basem
Published: September 2024

The continual progress pace of the development of the Wi-Fi generations may not fulfill the expected tremendous data growth of future smart-home network devices and applications. Throughout this article, we show a comprehensive design of the futuristic smart-home network that distributes massive data for indoor devices while fully supporting intelligent functions. We start by drawing a vision for various application scenarios of information and communication technologies in smart-home networks beyond the 2030s. Then, we appraise the technical specifications of the candidate intelligent home network. To achieve these technological speculations, we propose a novel holistic networking solution that is efficient and intelligent from service and operation perspectives. Specifically, we detail the proposed network architecture, elements, and enabling technologies. To accelerate the research progress and implementation, we further point out the future research directions. Finally, we discuss the non-technological factors that could affect the future deployment of the proposed network solution.
Advancing patient-centered cancer care: a systematic review of electronic patient-reported outcome measures
Authors: Salmani Hosna , Nasiri Somayeh , Alemrajabi Mahdi , Ahmadi Maryam
Published: September 2024

Background

Electronic Patient-Reported Outcome Measures (ePROMs) have emerged as valuable tools in cancer care, facilitating the comprehensive assessment of patients’ physical, psychological, and social well-being. This study synthesizes literature on the utilization of ePROMs in oncology, highlighting the diverse array of measurement instruments and questionnaires employed in cancer patient assessments. By comprehensively analyzing existing research, this study provides insights into the landscape of ePROMs, informs future research directions, and aims to optimize patient-centred oncology care through the strategic integration of ePROMs into clinical practice.

Methods

A systematic review was conducted by searching peer-reviewed articles published in academic journals without time limitations up to 2024. The search was performed across multiple electronic databases, including PubMed, Scopus, and Web of Science, using predefined search terms related to cancer, measurement instruments, and patient assessment. The selected articles underwent a rigorous quality assessment using the Mixed Methods Appraisal Tool (MMAT).

Results

The review of 85 studies revealed a diverse range of measurement instruments and questionnaires utilized in cancer patient assessments. Prominent instruments such as the European Organisation for Research and Treatment of Cancer Quality of Life Questionnaire Core 30 (EORTC QLQ-C30) and the Patient Reported Outcome-Common Terminology Criteria for Adverse Events (PRO-CTCAE) were frequently referenced across multiple studies. Additionally, other instruments identified included generic health-related quality of life measures and disease-specific assessments tailored to particular cancer types. The findings indicated the importance of utilizing a variety of measurement tools to comprehensively assess the multifaceted needs and experiences of cancer patients.

Conclusion

Our systematic review provides a comprehensive examination of the varied tools and ePROMs employed in cancer care, accentuating the perpetual requirement for development and validation. Prominent instruments like the EORTC QLQ-C30 and PRO-CTCAE are underscored, emphasizing the necessity for a thorough assessment to meet the multifaceted needs of patients. Looking ahead, scholarly endeavours should prioritize the enhancement of existing tools and the creation of novel measures to adeptly address the evolving demands of cancer patients across heterogeneous settings and populations.
N2-methylguanosine and N2, N2-dimethylguanosine in cytosolic and mitochondrial tRNAs
Authors: Petrosyan Jonny , Bohnsack Katherine E.
Published: September 2024

Decoration of cellular RNAs with modified RNA nucleosides is an important layer of gene expression regulation. Throughout the transcriptome, RNA modifications influence the folding, stability and function of RNAs as well as their interactions with RNA-binding proteins. Although first detected more than 50 years ago, the modified nucleosides N2-methylguanosine (m2G) and N2,N2-dimethylguanosine (m22G) have recently come to the fore through the identification and characterization of the human methyltransferases (MTases) responsible for their installation. In tRNAs, m2G and m22G are present at the junctions between the acceptor stem and the D-arm, and the D-arm and the anticodon stem loop. Here, we review the current knowledge on the effects of mono- and di-methylation of N2 of guanosine on base-pairing and provide an overview of m2(2)G sites in cytosolic and mitochondrial tRNAs. We highlight key features of m2G and m22G MTases, and describe how these enzymes specifically recognize their RNA substrates and target nucleosides. We also discuss the impact of these modifications on tRNA functions, their dynamic regulation and their implications in disease.
Nitrogen use efficiencies, flows, and losses of typical dairy farming systems in Inner Mongolia
Authors: Qili Moge , Zhao Zhanqing , Bao Junwei , Wu Nier , Gou Baomin , Ying Ying , Bilige Batu , Sun Lin , Xue Yanlin , Yang Fuyu
Published: September 2024

Dairy farming is a notable source of nitrogen (N) emissions, impacting both atmospheric and aquatic ecosystems, thus necessitating a detailed analysis of nutrient dynamics to curtail nutrient wastage. However, N flow variability and its environmental ramifications differ markedly among dairy farms, and a holistic understanding of these differences is lacking in Inner Mongolia, the biggest dairy production province in China. Utilizing data from 187 dairy farms and employing the NUFER-farm model, this study assessed N flows, N use efficiency (NUE), and N losses across four predominant dairy farming systems in Inner Mongolia. These systems include traditional pastoral dairy farms (PF), smallholder dairy farms with croplands (SF), industrial landless farms (IDF), and coupled dairy cattle and cropland-intensive farms (CDF). Our findings indicate considerable differences in N flows, NUE, and losses among the systems. On average, N deposition and N fertilizer were the primary N sources for PF and SF, respectively, whereas IDF and CDF derived over 90% of their N inputs from purchased feeds. PF and SF recycled all available manure N on-farm, whereas IDF and CDF recycled only approximately 36% of the total available manure N. N losses constituted 39–72% of total N outputs, with ammonia emissions accounting for 68–73% of total N losses across all farm types. In particular, PF had a higher N loss per kilogram of dairy product than other systems. Farm-level NUE ranged from 17 to 35%, with manure management practices showing significant variability, underscoring the potential for enhanced strategies to reduce N losses through improved manure treatment.
Wind Field Reconstruction Method Using Incomplete Wind Data Based on Vision Mamba Decoder Network
Authors: Chen Min , Wang Haonan , Chen Wantong , Ren Shiyu
Published: September 2024

Accurate meteorological information is crucial for the safety of civil aviation flights. Complete wind field information is particularly helpful for planning flight routes. To address the challenge of accurately reconstructing wind fields, this paper introduces a deep learning neural network method based on the Vision Mamba Decoder. The goal of the method is to reconstruct the original complete wind field from incomplete wind data distributed along air routes. This paper proposes improvements to the Vision Mamba model to fit our mission, showing that the developed model can accurately reconstruct the complete wind field. The experimental results demonstrate a mean absolute error (MAE) of wind speed of approximately 1.83 m/s, a mean relative error (MRE) of around 7.87%, an R-square value of about 0.92, and an MAE of wind direction of 5.78 degrees.
A Geographically Weighted Regression–Compute Unified Device Architecture Approach to Explore the Spatial Agglomeration and Heterogeneity in Arable Land Consumption in Southwest China
Authors: Liu Chang , Xu Tingting , Han Letao , Du Sapu , Tian Aohua
Published: September 2024

Arable land loss has become a critical issue in China because of rapid urbanization, industrial expansion, and unsustainable agricultural practices. While previous studies have explored the factors contributing to this loss, they often fall short in addressing the challenges of spatial heterogeneity and large-scale dataset analysis. This research introduces an innovative approach to geographically weighted regression (GWR) for assessing arable land loss in China, effectively addressing these challenges. Focusing on Chongqing, Guizhou, and Yunnan Provinces over the past two decades, it examines spatial autocorrelation with R-squared values exceeding 0.6 and residuals. Eight factors, including environmental elements (rain, evaporation, slope, digital elevation model) and human activities (distance to city, distance to roads, population, GDP), were analyzed. By visualizing and analyzing R² spatial patterns, the results reveal a clear spatial agglomeration distribution, primarily in urban areas with industries, highly urbanized cities, and flat terrains near rivers, influenced by GDP, population, rain, and slope. The novelty of this study is that it significantly enhances GWR computational capabilities for handling extensive datasets by utilizing Compute Unified Device Architecture (CUDA) on a high-performance GPU cloud server. Simultaneously, it conducts comprehensive analyses of the GWR model’s local results through visualization and spatial autocorrelation tools, enhancing the interpretability of the GWR model. Through spatial clustering analysis of local results, this study enables targeted exploration of factors influencing arable land changes in various temporal and spatial dimensions while also evaluating the reliability of the model results.
The Impact of Planting Dates on the Performance of Soybean Varieties [Glycine max (L.) Merr.] in the Nigerian Savannas
Authors: Eseigbe Osagie B. , Kamara Alpha Y. , Miko Sani , Omoigui Lucky O. , Solomon Reuben , Adeleke Musibau A. , Tofa Abdullahi I. , Bebeley Jenneh F.
Published: September 2024

Increasing delays in the onset of the rainy season and extended dry spells in the Nigerian savannas are complicating the determination of optimal planting dates for rain-fed crops, which increases risks for farmers. This study evaluated the impact of planting dates on soybean [Glycine max (L.) Merr.] performance to identify optimal planting dates for different soybean varieties in two agroecological zones (AEZs) of Nigeria. The study involved six planting dates (15 June, 22 June, 29 June, 6 July, 13 July, and 20 July) and three soybean varieties (TGX-1835-10E, TGX-1951-3F, TGX-1904-6F). Results showed significant differences in growth and yield parameters based on location, variety, and planting date. In the Sudan savanna (SS), AEZ at BUK-Kano, optimal yields (>1500 kg ha−1) were achieved when planting TGX-1835-10E and TGX-1951-3F from 15 to 29 June and TGX-1904-6F on 15 June. Planting beyond 29 June reduces yields by 12–55% for TGX-1835-10E and 27–63% for TGX-1951-3F. For TGX-1904-6F, planting after 15 June reduces yields by 27–90%. In the Northern Guinea savanna (NGS) AEZ at Zaria, optimal yields (>1500 kg ha−1) were obtained when planting TGX-1835-10E and TGX-1951-3F from 15 June to 6 July, and TGX-1904-6F between 15 to 29 June. Delaying planting beyond these dates significantly reduced yields by 18–31% for TGX-1835-10E and 12–20% for TGX-1951-3F and 10–41% for TGX-1904-6F.
Design and Testing of a Fruit Tree Variable Spray System Based on ExG-AABB
Authors: Sun Daozong , Quan Zhiwei , Wu Peiran , Liu Weikang , Xue Xiuyun , Song Shuran , Xie Jiaxing , Jiang Sheng
Published: September 2024

This paper addresses the issue of pesticide waste and low utilization rates resulting from traditional plant protection via spraying operations, which apply equal dosages to different targets or to different parts of the same target. To tackle this problem, we designed a variable fruit tree spraying system based on the ExG-AABB (excess green and axis-aligned bounding box) algorithm. We used a Kinect depth camera to capture information about the fruit tree canopy and constructed a spray flow model using pulse width modulation and variable spray control technology. Variable multi-nozzle spraying was guided by combining this canopy data. We evaluated the accuracy of each model in calculating canopy volume by comparing the coefficient of determination (R2) and root mean square error (RMSE) of the ExG-AABB with the slice convex hull method, voxel method, three-dimensional alpha-shape method, and QuickHull method. The ExG-AABB algorithm had the highest R2 value (0.9334) and the lowest RMSE value (0.0353 m3) among the five models, indicating that it most accurately reflects the true volume of the fruit tree canopy. This validates the effectiveness of the ExG-AABB algorithm in calculating canopy volume. We established a correlation model between canopy volume and spray volume, designed a canopy-adaptive layering method based on point cloud processing, and achieved precise calculation of nozzle flow. Comparative field experiments were conducted to analyze the spray coverage rate and observed flow, thereby evaluating the spraying effect of this variable spraying system. The experimental results showed that compared to conventional continuous spraying, this variable spraying system not only achieves more uniform spray coverage but also significantly reduces pesticide usage by 48.1%. Furthermore, through system optimization, the average coverage rate of the middle layer of the canopy decreased by 17.53%, effectively reducing the phenomenon of overlapping spraying from multiple nozzles and improving spraying efficiency.
Light Conditions and Auxin Influence the In Vitro Efficiency of Rooting and Acclimatization of Pennisetum ‘Vertigo®’
Authors: Prokopiuk Barbara , Kapczyńska Anna , Pawłowska Bożena
Published: September 2024

This paper reports an efficient in vitro rooting and acclimatization method for Pennisetum ‘Vertigo®’. For that purpose, the influence of the IAA (indole-3-acetic acid) in MS (Murashige and Skoog) media, and different types of LED light (100% blue, 100% red, a combination of red and blue (70% + 30%), a combination of red, blue with yellow, green, or far-red (35% + 15% + 50%), and white LED) were investigated. A fluorescent lamp and a medium without auxin were used as controls. Subsequently, the plants were transferred ex vitro in trays to the greenhouse and later transplanted into pots. In all tested combinations, the shoots rooted at a high rate of 70–100% (except under blue light). The best results were obtained under white LED or the fluorescent lamp, where the highest number of roots was formed; however, IAA did not increase the rooting rate, the number, or the weight of roots. Shoots rooted under LEDs and on media containing IAA generally exhibited higher soluble sugar content compared to those rooted on auxin-free media. Consistent with the rooting results, the best acclimatization was observed in plants rooted under white LED or fluorescent light on auxin-free media.
Surfactins and Iturins Produced by Bacillus velezensis Jt84 Strain Synergistically Contribute to the Biocontrol of Rice False Smut
Authors: Zhang Rongsheng , Yu Junjie , Yang Lin , Qiao Junqing , Qi Zhongqiang , Yu Mina , Du Yan , Song Tianqiao , Cao Huijuan , Pan Xiayan , Liu Youzhou , Liu Yongfeng
Published: September 2024

Rice false smut, caused by the plant pathogenic fungus Ustilaginoidea virens, is widespread in rice-growing regions globally, severely compromising rice quality and production. Employing Bacillus spp. to control rice false smut represents an effective and environmentally sustainable strategy for disease management. The lipopeptides produced by Bacillus velezensis Jt84 demonstrated robust inhibitory effects against U. virens, resulting in abnormal mycelial morphology and spore germination. Iturins were identified as essential for the antifungal activity against U. virens, as confirmed by mutagenesis experiments that suppressed iturin biosynthesis. The surfactin-deficient mutant exhibited inhibitory effects against U. virens comparable to the wild-type, indicating that the absence of surfactins did not diminish its antifungal activity. Both the Jt84∆srf and Jt84∆itu mutants displayed reduced biofilm formation capabilities compared to the wild-type, with the Jt84∆srf mutant being particularly impaired and unable to form a complete biofilm. Regarding swarming motility, the ∆srf mutant exhibited a significant reduction compared to the wild-type, whereas the Jt84∆itu mutant showed a modest increase. Colonization experiments revealed that the Jt84∆srf mutant strain had significantly lower colonization on rice leaf surfaces than the wild-type strain, highlighting the critical role of surfactins in the colonization of B. velezensis Jt84 on rice leaves. In conclusion, our research demonstrated that surfactins and iturins have distinct functionalities and act synergistically to contribute to the biocontrol of rice false smut in B. velezensis Jt84. This synergy is achieved through their potent antifungal effects, biofilm formation, and successful colonization.
Multiple Herbicide Resistance in Annual Ryegrass (Lolium rigidum Gaudin) in the Southeastern Cropping Region of Australia
Authors: Mahajan Gulshan , Chauhan Bhagirath Singh
Published: September 2024

Annual ryegrass (Lolium rigidum) is a problematic weed in winter crops and fallows in the southeastern cropping region (SCR) of Australia. This weed has evolved resistance to multiple herbicide groups, globally. In Australia, L. rigidum is more prevalent in the western and southern regions than in SCR. To assess the herbicide resistance status of L. rigidum, the response of five L. rigidum populations (collected from the SCR) to glyphosate, glufosinate, paraquat, haloxyfop-P-ethyl, and clethodim is determined using dose–response curves. Three parametric logistic models are used to determine the herbicide dose required to achieve 50% survival (LD50) and 50% growth reduction (GR50). The LD50 values for 50% survival at 28 days after treatment range from 1702 g a.e. ha−1 to 8225 g a.e. ha−1 for glyphosate, 1637 g a.i. ha−1 to 1828 g a.i. ha−1 for glufosinate, 141 g a.i. ha−1 to 307 g a.i. ha−1 for paraquat, 11 g a.i. ha−1 to 107 g a.i. ha−1 for haloxyfop-P-ethyl, and 17 g a.i. ha−1 to 48 g a.i. ha−1 for clethodim. The resistance factor, based on GR50 value, is highest in the S7 population (2.2 times) for glyphosate, the S11 population (2.3 times) for glufosinate, the S11 population (2.0 time) for paraquat, the S7 population (3.9 times) for haloxyfop-P-ethyl, and the S3 population (3.1 times) for clethodim, compared with the susceptible or less tolerant population. The S11 population is found to be resistant to five tested herbicides, based on resistance factors. Similarly, the S3 population is highly resistant to glyphosate, haloxyfop-P-ethyl, and clethodim compared with the W4 population. These results suggest that L. rigidum populations in the SCR exhibit resistance to multiple herbicide groups at labelled field rates. The findings highlight the necessity of adopting an integrated management approach, including the use of residual herbicides, tank mixing herbicides with different modes of action, and rotating herbicides in conjunction with cultural and mechanical control methods.
Rescue, Rehabilitation, and Feeding Schedule of a Franciscana Dolphin (Pontoporia blainvillei) Calf Stranded in Argentina
Authors: Loureiro Julio Daniel , Loureiro Juan Pablo , Rodriguez Heredia Sergio , von Fersen Lorenzo , Alvarez Karina Cecilia , Olocco Diz María Julieta , Nuñez Favre Romina
Published: September 2024

The franciscana dolphin (Pontoporia blainvillei) is a small cetacean endemic to the coastal waters of the southwestern Atlantic Ocean. Due to its restricted distribution, it is subject to high bycatch mortality in the gillnets used for commercial and artisanal fishing. The rehabilitation of the franciscana is still a major challenge, as most attempts to rehabilitate stranded animals have failed. This study aims to present the case of the rehabilitation of a franciscana dolphin calf, stranded in San Clemente del Tuyú, Argentina, at the beginning of the predation period. The feeding strategy and nutritional profile at different stages during the 88 days of rehabilitation are meticulously detailed. Its diet was prepared by hand based on studies of milk composition, the feeding ecology of franciscana in Argentinean waters, and previous records of the Fundación Mundo Marino Rehabilitation Center. The diets were designed to meet the nutritional needs of franciscana dolphins. In addition, the hematological, cytological, and fecal analyses recorded during the rehabilitation are presented. Although the animal could not be released, due to its death, this report provides baseline information that can improve the ability of veterinarians to care for debilitated, live-stranded dolphins. This information may also be useful in the implementation and development of healthcare protocols for this species.
Association of Encephalitozoon cuniculi with Clinical Signs and Abnormal Hematologic/Biochemical Changes in Pet Rabbits in Thailand
Authors: Duangurai Taksaon , Khamchomphu Natruree , Dusitkul Kanyanut , Tousee Chawaporn , Sukmai Yosanun , Rungnirundorn Teerapat , Areevijittrakul Ladawan , Jala Siriluk , Thengchaisri Naris
Published: September 2024

Encephalitozoon cuniculi can cause serious disease and subclinical infection in rabbits and requires active surveillance to control the infection. This study investigated the association between anti-Encephalitozoon cuniculi antibody status and various health parameters in pet rabbits. A total of 90 rabbits were divided into healthy (N = 30), subclinical (N = 30), and clinical (N = 30) groups based on their anti-Encephalitozoon cuniculi antibody status and clinical presentations. The mean ages of the control (37 ± 40 months) and subclinical groups (38 ± 34 months) were notably lower compared to that of the clinical group (63 ± 38 months, p < 0.01). Serum titers for anti-Encephalitozoon cuniculi antibodies were significantly elevated in rabbits with subclinical and clinical infections compared to those of healthy rabbits (p < 0.05). Neurological signs were predominant in rabbits with active E. cuniculi infection (80.0%), with additional pathological features including urinary dysfunction (10.0%) and cataracts (10.0%). The source of rabbits was not associated with E. cuniculi infection (p = 0.159). Anemia was significantly linked to E. cuniculi infection (p = 0.026); however, no significant associations were found with leukocytosis, thrombocytopenia, or serum biochemistry parameters. Mature adult rabbits were more likely to be infected with E. cuniculi. Recognizing anemia and neurological signs facilitates in early diagnosis of E. cuniculi infection.
Transcriptomic Study of Different Stages of Development in the Testis of Sheep
Authors: Xi Binpeng , Zhao Shengguo , Zhang Rui , Lu Zengkui , Li Jianye , An Xuejiao , Yue Yaojing
Published: September 2024

Numerous genes govern male reproduction, modulating testicular development and spermatogenesis. Our study leveraged RNA-Seq to explore candidate genes and pivotal pathways influencing fecundity in an F1 hybrid of Southdown × Hu sheep testes across four developmental milestones: M0 (0 months old, newborn), M3 (3 months old, sexually immature), M6 (6 months old, sexually mature), and Y1 (1 years old, adult). Histological examination using hematoxylins and eosin staining revealed that the cross-sectional area of the spermatid tubules and the number of supportive cells increased in the other groups, as compared to the M0 group. The cross-sectional area of the vasculature and the number of supporting cells were found to be significantly increased in all other groups in comparison to the M0 group. We conducted GO and KEGG analyses of the differentially expressed genes (DEGs) in the three comparison groups and identified key pathways, including cAMP, MAPK, ECM–receptor interactions, PI3K-Akt, and FOXO signaling, which are closely related to testicular development and spermatogenesis. Notably, alternative splicing (AS) events were markedly elevated in M6 and Y1 stages. Key genes like GATA4, GATA6, SMAD4, SOX9, YAP1, ITGB1 and MAPK1 emerged as significantly enriched in these pathways, potentially orchestrating the transition from immature to mature testes in sheep. These findings offer valuable insights into male reproductive potential and can inform strategies for optimizing animal breeding.
Making Noah’s Ark Work for Fishing Cat Conservation: A Blueprint for Connecting Populations across an Interactive Wild Ex Situ Spectrum
Authors: Adhya Tiasa , Singh Simran , Gottumukkala Himaja , Banerjee Aditya , Chongder Ishita , Maity Sulata , Reddy P.
Published: September 2024

The One Plan Approach advocates for a hybrid species management framework, wherein captive-bred populations are considered metapopulations nested within a broader network of zoos and wild populations Additionally, the Opportunities to Thrive framework aims to enhance animal welfare by addressing the physiological, psychological, and emotional needs of captive individuals, thereby improving conservation outcomes. Here, we present an integrated framework for the conservation of a globally threatened wetland wild cat species, the fishing cat, by synthesizing optimal ex situ management practices and in situ conservation strategies. Further, we examined the genetic constitution of the founder population in a fishing cat captive breeding program that was recently initiated by the West Bengal Zoo Authority, India and conducted a population viability analysis to suggest how best to maintain the genetic diversity of the population. We found that the present genetic diversity of 56% and maximum carrying capacity of the captive population (30 individuals) can be maintained for more than 100 years with a combination of supplementation and harvesting. Keeping stochastic events in mind, the introduction of two adult males and females to the existing population each year will seamlessly allow the harvesting of two adult males and two adult females every alternate year to supplement wild populations. Further, we adopted the proposed integrated framework to delineate recommendations for the supplementation of wild populations in West Bengal. We used environmental criteria known to influence fishing cat occurrence to identify 21 potential reintroduction zones in the Sundarbans landscape and Terai region in northern West Bengal with habitable areas for the fishing cat that are larger than the maximum known species’ home range. Our study is timely and insightful because it provides a holistic blueprint for implementing the One Plan Approach in safeguarding a threatened species.
Unveiling GFAP Astrocytopathy: Insights from Case Studies and a Comprehensive Review of the Literature
Authors: Gklinos Panagiotis , Athanasopoulos Fotios , Giatrakou Vagia , Arkoudis Nikolaos-Achilleas , Pournara Dorothea , Giagkou Eirini , Tountopoulou Argyro , Vassilopoulou Sofia , Mitsikostas Dimos-Dimitrios
Published: September 2024

Background: Autoimmune glial fibrillary acidic protein (GFAP) astrocytopathy, which was first identified in 2016, is an immune-mediated inflammatory disorder of the nervous system characterized by antibodies targeting GFAP. The exact pathogenic mechanisms, as well as the role of anti-GFAP antibodies, remain unclear; however, it seems that neuroinflammation is mediated by specific CD8+ T-cells and that neoplasms or viral infections can act as the initial trigger. Although the clinical spectrum of the disease is broad and heterogenous, GFAP astrocytopathy most commonly presents as meningoencephalitis with or without myelitis. Other symptoms include headache, visual disturbances, extrapyramidal or brainstem syndromes, and psychiatric manifestations including psychosis. The disease has a characteristically favorable response to steroid treatment while relapses occur in approximately 20–30% of the patients. Methods: We present two cases of GFAP astrocytopathy admitted to our hospital: a 43-year-old male with persistent headache and a 59-year-old female with acute dysarthria and swallowing difficulties followed by cognitive and behavioral symptoms. Results: Additionally, we conduct a comprehensive review of the literature to elucidate the role of anti-GFAP antibodies in disease pathogenesis and examine imaging characteristics, clinical manifestations, and treatment options for this recently described neuroimmunological condition. Conclusions: This review presents two unusual cases of GFAP-astrocytopathy and provides evidence for the pathogenesis, clinical presentation, imaging characteristics and treatment options of the disease.
Oxidative Stress in Fetuses and Newborns
Authors: Perrone Serafina , Tataranno Maria Luisa , Beretta Virginia , Buonocore Giuseppe , Gitto Eloisa
Published: September 2024

In recent years, significant research has uncovered new mechanisms by which molecules and substances that act as free radicals generate oxidative stress in the biological system, contributing to various forms of injury and disease [...]
Protective Effects of Anethole in Foeniculum vulgare Mill. Seed Ethanol Extract on Hypoxia/Reoxygenation Injury in H9C2 Heart Myoblast Cells
Authors: Seo Jeong Won , Habiba Sarmin Ummey , Munni Yeasmin Akter , Choi Ho Jin , Aktar Asma , Mazumder Kishor , Nah Deuk-Young , Yang In-Jun , Moon Il Soo
Published: September 2024

Background: Active compounds from plants and herbs are increasingly incorporated into modern medical systems to address cardiovascular diseases (CVDs). Foeniculum vulgare Mill., commonly known as fennel, is an aromatic medicinal plant and culinary herb that is popular worldwide. Methods: Protective effects against cellular damage were assessed in the H9C2 cardiomyocyte hypoxia/reoxygenation (H/R) experimental model. The identities of phytochemicals in FVSE were determined by GC-MS analysis. The phytochemical’s potential for nutrients and pharmacokinetic properties was assessed by ADMET analysis. Results: GC-MS analysis of the ethanol extracts of F. vulgare identified 41 bioactive compounds, with four prominent ones: anethole, 1-(4-methoxyphenyl)-2-propanone, ethoxydimethylphenylsilane, and para-anisaldehyde diethyl acetal. Among these, anethole stands out due to its potential for nutrients and pharmacokinetic properties assessed by ADMET analysis, such as bioavailability, lipophilicity, flexibility, and compliance with Lipinski’s Rule of Five. In the H/R injury model of H9C2 heart myoblast cells, FVSE and anethole suppressed H/R-induced reactive oxygen species (ROS) generation, DNA double-strand break damage, nuclear condensation, and the dissipation of mitochondrial membrane potential (ΔΨm). Conclusions: These findings highlight the therapeutic potential of FVSE and its prominent component, anethole, in the treatment of CVDs, particularly those associated with hypoxia-induced damage.