bluuebunny/crossref_metadata_2025 huggingface dataset:
Cortical Microtubule Organization during Petal Morphogenesis in Arabidopsis
Authors: Yang Yanqiu , Huang Weihong , Wu Endian , Lin Chentao , Chen Binqing , Lin Deshu
Published: October 2019

Cortical microtubules guide the direction and deposition of cellulose microfibrils to build the cell wall, which in turn influences cell expansion and plant morphogenesis. In the model plant Arabidopsis thaliana (Arabidopsis), petal is a relatively simple organ that contains distinct epidermal cells, such as specialized conical cells in the adaxial epidermis and relatively flat cells with several lobes in the abaxial epidermis. In the past two decades, the Arabidopsis petal has become a model experimental system for studying cell expansion and organ morphogenesis, because petals are dispensable for plant growth and reproduction. Recent advances have expanded the role of microtubule organization in modulating petal anisotropic shape formation and conical cell shaping during petal morphogenesis. Here, we summarize recent studies showing that in Arabidopsis, several genes, such as SPIKE1, Rho of plant (ROP) GTPases, and IPGA1, play critical roles in microtubule organization and cell expansion in the abaxial epidermis during petal morphogenesis. Moreover, we summarize the live-confocal imaging studies of Arabidopsis conical cells in the adaxial epidermis, which have emerged as a new cellular model. We discuss the microtubule organization pattern during conical cell shaping. Finally, we propose future directions regarding the study of petal morphogenesis and conical cell shaping.
Insulin-Like Growth Factor 2 (IGF2) Signaling in Colorectal Cancer—From Basic Research to Potential Clinical Applications
Authors: Kasprzak Aldona , Adamek Agnieszka
Published: October 2019

Colorectal cancer (CRC) is one of the most common cancers in men and women worldwide as well as is the leading cause of death in the western world. Almost a third of the patients has or will develop liver metastases. While genetic as well as epigenetic mechanisms are important in CRC pathogenesis, the basis of the most cases of cancer is unknown. High spatial and inter-patient variability of the molecular alterations qualifies this cancer in the group of highly heterogeneous tumors, which makes it harder to elucidate the mechanisms underlying CRC progression. Determination of highly sensitive and specific early diagnosis markers and understanding the cellular and molecular mechanism(s) of cancer progression are still a challenge of the current era in oncology of solid tumors. One of the accepted risk factors for CRC development is overexpression of insulin-like growth factor 2 (IGF2), a 7.5-kDa peptide produced by liver and many other tissues. IGF2 is the first gene discovered to be parentally imprinted. Loss of imprinting (LOI) or aberrant imprinting of IGF2 could lead to IGF2 overexpression, increased cell proliferation, and CRC development. IGF2 as a mitogen is associated with increased risk of developing colorectal neoplasia. Higher serum IGF2 concentration as well as its tissue overexpression in CRC compared to control are associated with metastasis. IGF2 protein was one of the three candidates for a selective marker of CRC progression and staging. Recent research indicates dysregulation of different micro- and long non-coding RNAs (miRNAs and lncRNAs, respectively) embedded within the IGF2 gene in CRC carcinogenesis, with some of them indicated as potential diagnostic and prognostic CRC biomarkers. This review systematises the knowledge on the role of genetic and epigenetic instabilities of IGF2 gene, free (active form of IGF2) and IGF-binding protein (IGFBP) bound (inactive form), paracrine/autocrine secretion of IGF2, as well as mechanisms of inducing dysplasia in vitro and tumorigenicity in vivo. We have tried to answer which molecular changes of the IGF2 gene and its regulatory mechanisms have the most significance in initiation, progression (including liver metastasis), prognosis, and potential anti-IGF2 therapy in CRC patients.
Gene Expression Profiling Reveals Enhanced Defense Responses in an Invasive Weed Compared to Its Native Congener During Pathogenesis
Authors: Manoharan Bharani , Qi Shan-Shan , Dhandapani Vignesh , Chen Qi , Rutherford Susan , Wan Justin SH , Jegadeesan Sridharan , Yang Hong-Yu , Li Qin , Li Jian , Dai Zhi-Cong , Du Dao-Lin
Published: October 2019

Invasive plants are a huge burden on the environment, and modify local ecosystems by affecting the indigenous biodiversity. Invasive plants are generally less affected by pathogens, although the underlying molecular mechanisms responsible for their enhanced resistance are unknown. We investigated expression profiles of three defense hormones (salicylic acid, jasmonic acid, and ethylene) and their associated genes in the invasive weed, Alternanthera philoxeroides, and its native congener, A. sessilis, after inoculation with Rhizoctonia solani. Pathogenicity tests showed significantly slower disease progression in A. philoxeroides compared to A. sessilis. Expression analyses revealed jasmonic acid (JA) and ethylene (ET) expressions were differentially regulated between A. philoxeroides and A. sessilis, with the former having prominent antagonistic cross-talk between salicylic acid (SA) and JA, and the latter showing weak or no cross-talk during disease development. We also found that JA levels decreased and SA levels increased during disease development in A. philoxeroides. Variations in hormonal gene expression between the invasive and native species (including interspecific differences in the strength of antagonistic cross-talk) were identified during R. solani pathogenesis. Thus, plant hormones and their cross-talk signaling may improve the resistance of invasive A. philoxeroides to pathogens, which has implications for other invasive species during the invasion process.
The Antitumor Effect of Lipophilic Bisphosphonate BPH1222 in Melanoma Models: The Role of the PI3K/Akt Pathway and the Small G Protein Rheb
Authors: Rittler Dominika , Baranyi Marcell , Molnár Eszter , Garay Tamás , Jalsovszky István , Varga Imre Károly , Hegedűs Luca , Aigner Clemens , Tóvári József , Tímár József , Hegedűs Balázs
Published: October 2019

Malignant melanoma is one of the most metastatic cancer types, and despite recent success with novel treatment strategies, there is still a group of patients who do not respond to any therapies. Earlier, the prenylation inhibitor hydrophilic bisphosphonate zoledronic acid (ZA) was found to inhibit melanoma growth in vitro, but only a weaker effect was observed in vivo due to its hydrophilic properties. Recently, lipophilic bisphosphonates (such as BPH1222) were developed. Accordingly, for the first time, we compared the effect of BPH1222 to ZA in eight melanoma lines using viability, cell-cycle, clonogenic and spheroid assays, videomicroscopy, immunoblot, and xenograft experiments. Based on 2D and spheroid assays, the majority of cell lines were more sensitive to BPH. The activation of Akt and S6 proteins, but not Erk, was inhibited by BPH. Additionally, BPH had a stronger apoptotic effect than ZA, and the changes of Rheb showed a correlation with apoptosis. In vitro, only M24met cells were more sensitive to ZA than to BPH; however, in vivo growth of M24met was inhibited more strongly by BPH. Here, we present that lipophilic BPH is more effective on melanoma cells than ZA and identify the PI3K pathway, particularly Rheb as an important mediator of growth inhibition.
Encapsulation of Micro- and Milli-Sized Particles with a Hollow-Type Spherical Bacterial Cellulose Gel via Particle-Preloaded Droplet Cultivation
Authors: Hoshi Toru , Suzuki Masashige , Ishikawa Mayu , Endo Masahito , Aoyagi Takao
Published: October 2019

A hollow-type spherical bacterial cellulose (HSBC) gel prepared using conventional methods cannot load particles larger than the pore size of the cellulose nanofiber network of bacterial cellulose (BC) gelatinous membranes. In this study, we prepared a HSBC gel encapsulating target substances larger than the pore size of the BC gelatinous membranes using two encapsulating methods. The first method involved producing the BC gelatinous membrane on the surface of the core that was a spherical alginate gel with a diameter of 2 to 3 mm containing the target substances. With this method, the BC gelatinous membrane was biosynthesized using Gluconacetobacter xylinus at the interface between the cell suspension attached onto the alginate gel and the silicone oil. The second method involved producing the BC gel membrane on the interface between the silicone oil and cell suspension, as well as the spherical alginate gel with a diameter of about 1 mm containing target substances. After the BC gelatinous membrane was biosynthesized, an alginate gel was dissolved in a phosphate buffer to prepare an HSBC gel with the target substances. These encapsulated substances could neither pass through the BC gelatinous membrane of the HSBC gel nor leak from the interior space of the HSBC gel. These results suggest that the HSBC gel had a molecular sieving function. The HSBC gel walls prepared using these methods were observed to be uniform and would be useful for encapsulating bioactive molecules, such as immobilized enzymes in HSBC gel, which is expected to be used as a drug carrier.
Novel SCN5A p.W697X Nonsense Mutation Segregation in a Family with Brugada Syndrome
Authors: Micaglio Emanuele , Monasky Michelle , Resta Nicoletta , Bagnulo Rosanna , Ciconte Giuseppe , Giannelli Luigi , Locati Emanuela , Vicedomini Gabriele , Borrelli Valeria , Ghiroldi Andrea , Anastasia Luigi , Benedetti Sara , Di Resta Chiara , Ferrari Maurizio , Pappone Carlo
Published: October 2019

Brugada syndrome (BrS) is marked by an elevated ST-segment elevation and increased risk of sudden cardiac death. Variants in the SCN5A gene are considered to be molecular confirmation of the syndrome in about one third of cases, while the genetics remain a mystery in about half of the cases, with the remaining cases being attributed to variants in any of a number of genes. Before research models can be developed, it is imperative to understand the genetics in patients. Even data from humans is complicated, since variants in the most common gene in BrS, SCN5A, are associated with a number of pathologies, or could even be considered benign, depending on the variant. Here, we provide crucial human data on a novel NM_198056.2:c.2091G>A (p.Trp697X) point-nonsense heterozygous variant in the SCN5A gene, as well as its segregation with BrS. The results herein suggest a pathogenic effect of this variant. These results could be used as a stepping stone for functional studies to better understand the molecular effects of this variant in BrS.
Scaffold-Free 3-D Cell Sheet Technique Bridges the Gap between 2-D Cell Culture and Animal Models
Authors: Alghuwainem Ayidah , Alshareeda Alaa T. , Alsowayan Batla
Published: October 2019

Various tissue engineering techniques have been created in research spanning two centuries, resulting in new opportunities for growing cells in culture and the creation of 3-D tissue-like constructs. These techniques are classified as scaffold-based and scaffold-free techniques. Cell sheet, as a scaffold-free technique, has attracted research interest in the context of drug discovery and tissue repair, because it provides more predictive data for in vivo testing. It is one of the most promising techniques and has the potential to treat degenerative tissues such as heart, kidneys, and liver. In this paper, we argue the advantages of cell sheets as a scaffold-free approach, compared to other techniques, including scaffold-based and scaffold-free techniques such as the classic systemic injection of cell suspension.
Human Umbilical Vein Endothelial Cells (HUVECs) Co-Culture with Osteogenic Cells: From Molecular Communication to Engineering Prevascularised Bone Grafts
Authors: Kocherova Ievgeniia , Bryja Artur , Mozdziak Paul , Angelova Volponi Ana , Dyszkiewicz-Konwińska Marta , Piotrowska-Kempisty Hanna , Antosik Paweł , Bukowska Dorota , Bruska Małgorzata , Iżycki Dariusz , Zabel Maciej , Nowicki Michał , Kempisty Bartosz
Published: October 2019

The repair of bone defects caused by trauma, infection or tumor resection is a major clinical orthopedic challenge. The application of bone grafts in orthopedic procedures is associated with a problem of inadequate vascularization in the initial phase after implantation. Meanwhile, the survival of cells within the implanted graft and its integration with the host tissue is strongly dependent on nutrient and gaseous exchange, as well as waste product removal, which are effectuated by blood microcirculation. In the bone tissue, the vasculature also delivers the calcium and phosphate indispensable for the mineralization process. The critical role of vascularization for bone healing and function, led the researchers to the idea of generating a capillary-like network within the bone graft in vitro, which could allow increasing the cell survival and graft integration with a host tissue. New strategies for engineering pre-vascularized bone grafts, that apply the co-culture of endothelial and bone-forming cells, have recently gained interest. However, engineering of metabolically active graft, containing two types of cells requires deep understanding of the underlying mechanisms of interaction between these cells. The present review focuses on the best-characterized endothelial cells—human umbilical vein endothelial cells (HUVECs)—attempting to estimate whether the co-culture approach, using these cells, could bring us closer to development and possible clinical application of prevascularized bone grafts.
Adrenal Insufficiency in Patients with Corticosteroid-Refractory Cerebral Radiation Necrosis Treated with Bevacizumab
Authors: Voss Martin , Batarfi AbdulAziz , Steidl Eike , Wagner Marlies , Forster Marie-Thérèse , Steinbach Joachim P. , Rödel Claus M. , Bojunga Jörg , Ronellenfitsch Michael W.
Published: October 2019

Cerebral radiation necrosis is a common complication of the radiotherapy of brain tumours that can cause significant mortality. Corticosteroids are the standard of care, but their efficacy is limited and the consequences of long-term steroid therapy are problematic, including the risk of adrenal insufficiency (AI). Off-label treatment with the vascular endothelial growth factor A antibody bevacizumab is highly effective in steroid-resistant radiation necrosis. Both the preservation of neural tissue integrity and the cessation of steroid therapy are key goals of bevacizumab treatment. However, the withdrawal of steroids may be impossible in patients who develop AI. In order to elucidate the frequency of AI in patients with cerebral radiation necrosis after treatment with corticosteroids and bevacizumab, we performed a retrospective study at our institution’s brain tumour centre. We obtained data on the tumour histology, age, duration and maximum dose of dexamethasone, radiologic response to bevacizumab, serum cortisol, and the need for hydrocortisone substitution for AI. We identified 17 patients with cerebral radiation necrosis who had received treatment with bevacizumab and had at least one available cortisol analysis. Fifteen patients (88%) had a radiologic response to bevacizumab. Five of the 17 patients (29%) fulfilled criteria for AI and required hormone substitution. Age, duration of dexamethasone treatment, and time since radiation were not statistically associated with the development of AI. In summary, despite the highly effective treatment of cerebral radiation necrosis with bevacizumab, steroids could yet not be discontinued due to the development of AI in roughly one-third of patients. Vigilance to spot the clinical and laboratory signs of AI and appropriate testing and management are, therefore, mandated.
Predictors and Clinical Outcomes in Empyema Thoracis Patients Presenting to the Emergency Department Undergoing Video-Assisted Thoracoscopic Surgery
Authors: Tsai Yuan-Ming , Gamper Nikita , Huang Tsai-Wang , Lee Shih-Chun , Chang Hung
Published: October 2019

Background: Video-assisted thoracoscopic surgery (VATS) is widely used for the treatment of empyema. We evaluated clinical symptoms, laboratory examinations, and thoracentesis to assess patients in the emergency department (ED) with empyema thoracis, undergoing VATS to identify predictors of adverse outcomes. Methods: This retrospective study was conducted by reviewing records of ED patients with pleural empyema admitted for VATS from January 2007 to June 2014. Demographic data, clinical symptoms, and laboratory examinations were compared for survivors (Group I) and non-survivors (Group II). Logistic regression analysis was used to identify parameters related to postoperative mortality. Results: From 380 patients, 7.6% (n = 29) died postoperatively. Survivors and non-survivors exhibited differences in age, gender, presence of cough, dyspnea, chest pain, empyema stage, cerebrovascular disease, malignancy, the glucose level of pleural fluid, serum hemoglobin, platelet count, blood urea nitrogen, and potassium levels. The logistic analysis demonstrated that the most significant factor related to the postoperative morbidity is chest pain (p = 0.018). Conclusions: VATS could be a safe option for pediatric and geriatric patients. Age does not appear to affect postoperative mortality. A high degree of awareness is essential for perioperative management and early surgical treatment when ED patients present with the clinical symptom of chest pain.
Sub-Inhibitory Clindamycin and Azithromycin reduce S. aureus Exoprotein Induced Toxicity, Inflammation, Barrier Disruption and Invasion
Authors: Hu Hua , Ramezanpour Mahnaz , Hayes Andrew J , Liu Sha , Psaltis Alkis J , Wormald Peter-John , Vreugde Sarah
Published: October 2019

Background: Chronic rhinosinusitis (CRS) is defined as a chronic inflammation of the nose and paranasal sinus mucosa associated with relapsing infections—particularly with S. aureus. Long-term treatments with protein synthesis inhibitor antibiotics have been proposed to reduce inflammation in the context chronic severe inflammatory airway pathologies, including CRS. This study assessed the effect of subinhibitory clindamycin and azithromycin on S. aureus exoprotein induced inflammation, toxicity and invasiveness. Methods: S. aureus ATCC51650 and two clinical isolates grown in planktonic and biofilm form were treated with subinhibitory clindamycin and azithromycin. Exoproteins were collected and applied to primary human nasal epithelial cells (HNECs) in monolayers and at air-liquid interface. This was followed by lactate dehydrogenase (LDH), enzyme-linked immunosorbent assay (ELISA), Transepithelial Electrical Resistance (TEER) and paracellular permeability assays to assess the effect on cell toxicity, inflammatory cytokine production and mucosal barrier structure and function, respectively. The effect of these treatments was tested as well on the S. aureus invasiveness of HNECs. Results: Subinhibitory clindamycin reduced S. aureus exoprotein production in planktonic and biofilm form, thereby blocking exoprotein-induced toxicity, reversing its detrimental effects on mucosal barrier structure and function and modulating its inflammatory properties. Sub-inhibitory azithromycin had similar effects—albeit to a lesser extent. Furthermore, clindamycin—but not azithromycin—treated S. aureus lost its invasive capacity of HNECs. Conclusion: Subinhibitory clindamycin and azithromycin reduce S. aureus exoprotein production, thereby modulating the inflammatory cascade by reducing exoprotein-induced toxicity, inflammation, mucosal barrier disruption and invasiveness.
Non-Vitamin K Antagonist Oral Anticoagulants in Patients with Atrial Fibrillation and Valvular Heart Disease
Authors: Moon Inki , Lee So-Ryoung , Choi Eue-Keun , Lee Euijae , Jung Jin-Hyung , Han Kyung-Do , Cha Myung-Jin , Oh Seil , Lip Gregory Y.H.
Published: October 2019

Background: There are limited data for non-vitamin K antagonist oral anticoagulants (NOACs) impact on outcomes for patients with atrial fibrillation (AF) and valvular heart diseases (VHDs). Methods: We identified patients with AF and associated Evaluated Heartvalves, Rheumatic or Artificial (EHRA) type 2 VHDs, and who had been naïve from the oral anticoagulants in the Korean National Health Insurance Service database between 2014 and 2016 (warfarin: n = 2671; NOAC: n = 3058). For analyzing the effect of NOAC on primary prevention, we excluded those with a previous history of ischemic stroke, intracranial hemorrhage (ICH), and gastrointestinal (GI) bleeding events. To balance covariates, we used the propensity score weighting method. Ischemic stroke, ICH, GI bleeding, major bleeding, all-cause death, and their composite outcome and fatal clinical events were evaluated. Results: During a follow-up with a mean duration of 1.4 years, NOACs were associated with lower risks of ischemic stroke (hazard ratio (HR): 0.71, 95% confidence interval (CI): 0.53–0.96), GI bleeding (HR: 0.50, 95% CI: 0.35–0.72), fatal ICH (HR: 0.28, 95% CI: 0.07–0.83), and major bleeding (HR: 0.61, 95% CI: 0.45–0.80) compared with warfarin. Overall, NOACs were associated with a lower risk of the composite outcome (HR: 0.68, 95% CI: 0.58–0.80). Conclusions: In this nationwide Asian AF population with EHRA type 2 VHDs, NOAC use was associated with lower risks of ischemic stroke, major bleeding, all-cause death, and the composite outcome compared to warfarin use.
Preeclampsia: Risk Factors, Diagnosis, Management, and the Cardiovascular Impact on the Offspring
Authors: Fox Rachael , Kitt Jamie , Leeson Paul , Aye Christina Y.L. , Lewandowski Adam J.
Published: October 2019

Hypertensive disorders of pregnancy affect up to 10% of pregnancies worldwide, which includes the 3%–5% of all pregnancies complicated by preeclampsia. Preeclampsia is defined as new onset hypertension after 20 weeks’ gestation with evidence of maternal organ or uteroplacental dysfunction or proteinuria. Despite its prevalence, the risk factors that have been identified lack accuracy in predicting its onset and preventative therapies only moderately reduce a woman’s risk of preeclampsia. Preeclampsia is a major cause of maternal morbidity and is associated with adverse foetal outcomes including intra-uterine growth restriction, preterm birth, placental abruption, foetal distress, and foetal death in utero. At present, national guidelines for foetal surveillance in preeclamptic pregnancies are inconsistent, due to a lack of evidence detailing the most appropriate assessment modalities as well as the timing and frequency at which assessments should be conducted. Current management of the foetus in preeclampsia involves timely delivery and prevention of adverse effects of prematurity with antenatal corticosteroids and/or magnesium sulphate depending on gestation. Alongside the risks to the foetus during pregnancy, there is also growing evidence that preeclampsia has long-term adverse effects on the offspring. In particular, preeclampsia has been associated with cardiovascular sequelae in the offspring including hypertension and altered vascular function.
A Virus-Derived Immune Modulating Serpin Accelerates Wound Closure with Improved Collagen Remodeling
Authors: Zhang Liqiang , Yaron Jordan R. , Tafoya Amanda M. , Wallace Sarah E. , Kilbourne Jacquelyn , Haydel Shelley , Rege Kaushal , McFadden Grant , Lucas Alexandra R.
Published: October 2019

Numerous treatments have been developed to promote wound healing based on current understandings of the healing process. Hemorrhaging, clotting, and associated inflammation regulate early wound healing. We investigated treatment with a virus-derived immune modulating serine protease inhibitor (SERPIN), Serp-1, which inhibits thrombolytic proteases and inflammation, in a mouse excisional wound model. Saline or recombinant Serp-1 were applied directly to wounds as single doses of 1 μg or 2 µg or as two 1 µg boluses. A chitosan-collagen hydrogel was also tested for Serp-1 delivery. Wound size was measured daily for 15 days and scarring assessed by Masson’s trichrome, Herovici’s staining, and immune cell dynamics and angiogenesis by immunohistochemistry. Serp-1 treatment significantly accelerated wound healing, but was blocked by urokinase-type plasminogen activator (uPAR) antibody. Repeated dosing at a lower concentration was more effective than single high-dose serpin. A single application of Serp-1-loaded chitosan-collagen hydrogel was as effective as repeated aqueous Serp-1 dosing. Serp-1 treatment of wounds increased arginase-1-expressing M2-polarized macrophage counts and periwound angiogenesis in the wound bed. Collagen staining also demonstrated that Serp-1 improves collagen maturation and organization at the wound site. Serp-1 has potential as a safe and effective immune modulating treatment that targets thrombolytic proteases, accelerating healing and reducing scar in deep cutaneous wounds.
Total Tear IgE Levels Correlate with Allergenic and Irritating Environmental Exposures in Individuals with Dry Eye
Authors: Dermer Harrison , Theotoka Despoina , Lee Charity J. , Chhadva Priyanka , Hackam Abigail S. , Galor Anat , Kumar Naresh
Published: October 2019

Dry eye (DE) and allergic conjunctivitis may present similarly, and it remains unclear whether some individuals have an underlying allergic component to their DE. To better understand this relationship, we performed a cross-sectional study in 75 individuals with DE symptoms and/or signs. Immunoglobulin E (IgE) levels in tear samples were quantified and home environmental exposures assessed via standardized survey. Tears were collected by Schirmer strip, and total tear IgE levels were quantified using enzyme-linked immunosorbent assay (ELISA). Data were analyzed using descriptive statistics and linear and logistic regressions. The main outcome measures were total tear IgE levels and their association with environmental exposures. The mean age of the subjects was 66.2 ± 7.8 years. Sixty-two individuals had dry eye symptoms (Dry Eye Questionnaire-5 ≥ 6), and 75 had one or more signs of DE. Detectable total tear IgE levels were observed in 76% of subjects, and 17.3% had high levels (>1 ng/mL). Individuals with exposure to pet(s) (odds ratio (OR) 11.5, p = 0.002) and smoke (OR 38.6, p = 0.008) at home were more likely to have high IgE levels compared to those not exposed. Individuals with tears collected during spring or summer were 3.9 times (p = 0.028) more likely to have high IgE compared to those sampled at other times of year. Subjects born in the US were 3.45 times (p = 0.010) more likely to have high IgE compared to individuals born outside the US. To conclude, a majority of individuals with DE symptoms and/or signs had detectable IgE levels in their tears. High tear IgE levels were correlated with allergy season and exposures in the home linked with allergy.
Climate Change Impact in the Ria de Aveiro Lagoon Ecosystem: A Case Study
Authors: Lopes José Fortes , Lopes Carina Lurdes , Dias João Miguel
Published: October 2019

Climate change and global sea-level rise are major issues of the 21st century. The main goal of this study is to assess the physical and biogeochemical status of the Ria de Aveiro lagoon (Portugal) under future climate scenarios, using a coupled physical/ eutrophication model. The impact on the lagoon ecosystem status of the mean sea level rise (MSLR), the amplitude rise of the M2 tidal constituent (M2R), the changes in the river discharge, and the rising of the air temperature was investigated. Under MSLR and M2R, the results point to an overall salinity increase and water temperature decrease, revealing ocean water dominance. The main lagoon areas presented salinity values close to those of the ocean waters (~34 PSU), while a high range of salinity was presented for the river and the far end areas (20–34 PSU). The water temperature showed a decrease of approximately 0.5–1.5 °C. The responses of the biogeochemical variables reflect the increase of the oceanic inflow (transparent and nutrient-poor water) or the reduction of the river flows (nutrient-rich waters). The results evidenced, under the scenarios, an overall decreasing of the inorganic nitrogen concentration and the carbon phytoplankton concentrations. A warm climate, although increasing the water temperature, does not seem to affect the lagoon’s main status, at least in the frame of the model used in the study.
A Study on the Estimation of Facilities in LNG Bunkering Terminal by Simulation—Busan Port Case
Authors: Park Nam Kyu , Park Sang Kook
Published: October 2019

Since 2020, the International Maritime Organization (IMO) has tightened regulations on the emissions of sulfur oxides from ships from less than 3.5% to less than 0.5%. As a countermeasure, shipping companies can adopt one of three potential solutions: using low sulfur fuel (LSFO), installing scrubbers, or using liquefied natural gas (LNG) fuel. However, considering the environmental aspects such as the UN greenhouse gas (GHG) emission reduction program and the reduction of fine dust generation in port areas, LNG fuel is ultimately considered to be the most ideal method in the marine industry. In line with this international trend, major port authorities are considering building LNG bunkering stations, but the proper methods and criteria for estimating the size of LNG bunkering infrastructure are not clear. This study proposes a method of estimating the size of LNG infrastructure required with consideration for the operational status of ports according to the estimated amount of bunkering demand at a future time with the case study of Busan Port in Korea. In order to estimate the detailed demand amount by inbound vessels, a simulation modeling technique is applied as a tool of research.
Multivariable Control of Solar Battery Power by Extremum Seeking: Starting from Linear Analysis
Authors: Kirpichnikova I. M. , Sologubov A. Yu.
Published: October 2019

In this study, we tried to combine maximum power point trackers (MPPT) and «Extremum Seeking» in a single multi-parameter extremum seekeng system for orienting solar panels and draw attention to the problem of a deeper study of nonlinear adaptive control using appropriate methods for their analysis. MPPT controller becomes one of the extremum seeking loops, and as a result, the maximum power is achieved not only by searching for the optimal voltage value, but also due to the optimal angular position of the solar panel in Euclidean space, because the photocurrent depends on the angle of inclination of the Sun’s rays to the surface. The task of tuning extremum seeking loops becomes more analytically difficult, which is associated with nonlinear and multiply connected properties. This requires starting the solution from a simpler “linear” level. We applied the approach associated with the passage of modulating oscillations with a given frequency and amplitude through an open-loop system. This approach, which is generalized in this work at least for extremum seeking of the solar panels power, should be used for approximate calculations if there are no strict requirements for convergence and energy loss for the search. Research design is as follows: parametric identification of the current-voltage and volt-watt curves; obtaining the transfer function by the semi-automated sparse matrix method; reducing the order of the transfer function of coordinate electric drives by introducing a scaling factor. To the most important theoretical result, we attribute the property of the generalized amplitude of the solar panel power oscillations with multi-parameter control to be a combination of input modulating oscillations superimposed on the signals of the control integrators. Having revealed the relationship of their properties, it becomes possible to eliminate non-linearity from the system and operate only with the analytical relationship of the input modulating oscillations and the generalized oscillation of the controlled parameter. We attribute the prediction of the effect to one of the most interesting physical results, in which, for the same amplitude of modulating oscillations, the amplitudes of the photocurrent oscillations and the power of the solar panel at different angular positions will be generally different.
Approximate Solutions of Time Fractional Diffusion Wave Models
Authors: Ghafoor Abdul , Haq Sirajul , Hussain Manzoor , Kumam Poom , Jan Muhammad Asif
Published: October 2019

In this paper, a wavelet based collocation method is formulated for an approximate solution of (1 + 1)- and (1 + 2)-dimensional time fractional diffusion wave equations. The main objective of this study is to combine the finite difference method with Haar wavelets. One and two dimensional Haar wavelets are used for the discretization of a spatial operator while time fractional derivative is approximated using second order finite difference and quadrature rule. The scheme has an excellent feature that converts a time fractional partial differential equation to a system of algebraic equations which can be solved easily. The suggested technique is applied to solve some test problems. The obtained results have been compared with existing results in the literature. Also, the accuracy of the scheme has been checked by computing L 2 and L ∞ error norms. Computations validate that the proposed method produces good results, which are comparable with exact solutions and those presented before.
On Mann Viscosity Subgradient Extragradient Algorithms for Fixed Point Problems of Finitely Many Strict Pseudocontractions and Variational Inequalities
Authors: Ceng Lu-Chuan , Petruşel Adrian , Yao Jen-Chih
Published: October 2019

In a real Hilbert space, we denote CFPP and VIP as common fixed point problem of finitely many strict pseudocontractions and a variational inequality problem for Lipschitzian, pseudomonotone operator, respectively. This paper is devoted to explore how to find a common solution of the CFPP and VIP. To this end, we propose Mann viscosity algorithms with line-search process by virtue of subgradient extragradient techniques. The designed algorithms fully assimilate Mann approximation approach, viscosity iteration algorithm and inertial subgradient extragradient technique with line-search process. Under suitable assumptions, it is proven that the sequences generated by the designed algorithms converge strongly to a common solution of the CFPP and VIP, which is the unique solution to a hierarchical variational inequality (HVI).
Diuretic Enhanced Ultrasonography in the Diagnosis of Pyeloureteral Obstruction
Authors: Kazlauskas Vytis , Cekuolis Andrius , Bilius Vytautas , Anglickis Marius , Verkauskas Gilvydas
Published: October 2019

Background and Objectives: To determine the value of diuretic ultrasonography for the diagnosis of obstructive hydronephrosis. Materials and Methods: Diuretic enhanced ultrasonography was used routinely as a part of examination of patients with hydronephrosis in our Department. There were 72 patients (42 males, 30 females; aged 2 months to 17 years; median age 7.07 years) with a sonoscopic diagnosis of hydronephrosis included from January 2006 until October 2011. The anteroposterior diameter (AD) of renal pelvis was measured sonoscopically before and at sixty minutes after furosemide injection. A weight-adjusted dose of 1 mg/kg of furosemide was administered intravenously. Results: Patients were operated on if pyeloureteral obstruction was suspected because of low or deteriorating differential renal function, increasing hydronephrosis or symptoms thereof. Hydronephrosis was unilateral in 61 (84.7%) and bilateral in 11 (15.3%) patients. The median AD of pelvis before furosemide injection was 22 mm in operated and 17 mm in non-operated patients (p = 0.005). Sixty minutes after furosemide injection, the AD of pelvis in operated patients was 35.5 mm and 25.8 mm in non-operated—25.8 mm (p < 0.001). Logistic regression model demonstrated that significant factors for surgery were: AD 60 min after furosemide infection and ultrasonographic parenchymal sclerosis. Conclusion: Ultrasound measurement of the AD of renal pelvis 1 h after the injection of furosemide used as an additional investigation can help in predicting obstructive hydronephrosis.
Metastatic Lymph Node Ratio (mLNR) is a Useful Parameter in the Prognosis of Colorectal Cancer; A Meta-Analysis for the Prognostic Role of mLNR
Authors: Pyo Jung Soo , Kim Joo Heon , Lee Seung Yun , Baek Tae Hwa , Kang Dong Wook
Published: October 2019

Background and objectives: The presenting study aimed to elucidate the prognostic role of the metastatic lymph node ratio (mLNR) in patients with colorectal cancer (CRC), using a meta-analysis. Materials and Methods: Using data from 90,274 patients from 14 eligible studies, we performed a meta-analysis for the correlation between mLNR and survival rate. Besides, subgroup analyses were performed, based on tumor stage, tumor location, and mLNR. Results: A high mLNR showed significant correlation with worse overall survival and disease-free survival rates in CRC patients (hazard ratio (HR), 1.617, 95% confidence interval (CI) 1.393–1.877, and HR 2.345, 95% CI 1.879–2.926, respectively). In patients with stage III, who had regional LN metastasis, the HRs were 1.730 (95% CI 1.266–2.362) and 2.451 (95% CI 1.719–3.494) for overall and disease-free survival, respectively. According to tumor location, rectal cancer showed a worse survival rate when compared to colon cancer. In the analysis for overall survival, when mLNR was 0.2, HR was the highest across the different subgroups (HR 5.040, 95% CI 1.780–14.270). However, in the analysis for disease-free survival, the subgroup with an mLNR < 0.2 had a higher HR than the other subgroups (HR 2.878, 95% CI 1.401–5.912). Conclusions: The mLNR may be a useful prognostic factor for patients with CRC, regardless of the tumor stage or tumor location. Further studies are necessary for the detailed criteria of mLNR before its application in daily practice.
Bacterial and Fungal Diversity Inside the Medieval Building Constructed with Sandstone Plates and Lime Mortar as an Example of the Microbial Colonization of a Nutrient-Limited Extreme Environment (Wawel Royal Castle, Krakow, Poland)
Authors: Dyda Magdalena , Pyzik Adam , Wilkojc Ewa , Kwiatkowska-Kopka Beata , Sklodowska Aleksandra
Published: October 2019

Biodeterioration is a serious threat to cultural heritage objects and buildings. The deterioration of a given material often incurs irreparable losses in terms of uniqueness and historical value. Hence preventive actions should be taken. One important challenge is to identify microbes involved in the biodeterioration process. In this study, we analyzed the microbial diversity of an ancient architectonical structure of the Rotunda of Sts. Felix and Adauctus, which is a part of the Wawel Royal Castle located in Krakow, Poland. The Rotunda is unavailable to tourists and could be treated as an extreme habitat due to the low content of nutrients coming either from sandstone plates bound with lime mortar or air movement. Microbial diversity was analyzed with the use of the high-throughput sequencing of marker genes corresponding to fragments of 16S rDNA (for Bacteria) and ITS2 (internal transcribed spacer 2) (for Fungi). The results showed that the microbial community adhered to wall surfaces is, to a large extent, endemic. Furthermore, alongside many microorganisms that could be destructive to masonry and mortar (e.g., Pseudomonas, Aspergillus), there were also bacteria, such as species of genera Bacillus, Paenisporosarcina, and Amycolatopsis, that can positively affect wall surface properties by reducing the damage caused by the presence of other microorganisms. We also showed that airborne microorganisms probably have little impact on the biodeterioration process as their abundance in the microbial community adhered to the ancient walls was very low.
Comment on “Shiga-Toxin Producing Escherichia coli in Brazil: A Systematic Review. Microorganisms 2019, 7, 137”
Authors: Guth Beatriz E. C.
Published: October 2019

A recent article by Castro et al. describes a systematic review of Shiga-toxin producingEscherichia coli (STEC) in Brazil. [...]
Design of a pH-Responsive Conductive Nanocomposite Based on MWCNTs Stabilized in Water by Amphiphilic Block Copolymers
Authors: den Hoed Frank , Pucci Andrea , Picchioni Francesco , Raffa Patrizio
Published: October 2019

Homogeneous water dispersions of multi-walled carbon nanotubes (MWCNTs) were prepared by ultrasonication in the presence of an amphiphilic polystyrene-block-poly(acrylic acid) (PS-b-PAA) copolymer. The ability of PS-b-PAA to disperse and stabilize MWCTNs was investigated by UV-vis, SEM and zeta potential. The results show that the addition of a styrene block to PAA enhances the dispersion efficiency of the graphitic filler compared to pure PAA, possibly due to the nanotube affinity with the polystyrene moiety. Notably, the dispersions show an evident pH-responsive behavior, being MWCNTs reaggregation promoted in basic environment. It is worth noting that the responsive character is maintained in solid composites obtained by drop casting, thus indicating potential applications in sensing.
Surface Carbon Shell-Functionalized ZrO2 as Nanofiller in Polymer Gel Electrolyte-Based Dye-Sensitized Solar Cells
Authors: Lim Seung Man , Moon Juyoung , Choi Gyo Hun , Baek Uoon Chul , Lim Jeong Min , Park Jung Tae , Kim Jong Hak
Published: October 2019

We prepare dye-sensitized solar cells (DSSCs) fabricated with a poly (ethylene glycol) based polymer gel electrolytes (PGEs) incorporating surface carbon shell-functionalized ZrO2 nanoparticles (ZrO2-C) as nanofillers (NFs). ZrO2 are polymerized via atom transfer radical polymerization (ATRP) using poly (ethylene glycol) methyl ether methacrylate (POEM) as a scaffold to prepare the ZrO2-C through carbonization. The power conversion efficiency of DSSC with 12 wt% ZrO2-C/PGEs is 5.6%, exceeding that with PGEs (4.4%). The enhanced efficiency is attributed to Lewis acid-base interactions of ZrO2-C and poly (ethylene glycol), catalytic effect of the carbon shells of ZrO2-C, which results in reduced crystallinity, enhanced ion conductivity of electrolytes, decreased counterelectrode/electrolyte interfacial resistance, and improved charge transfer rate. These results demonstrate that ZrO2-C introduction to PGEs effectively improves the performance of DSSCs.
Equol Pretreatment Protection of SH-SY5Y Cells against Aβ (25–35)-Induced Cytotoxicity and Cell-Cycle Reentry via Sustaining Estrogen Receptor Alpha Expression
Authors: Tsai Meng-Chao , Lin Shyh-Hsiang , Hidayah Kiswatul , Lin Ching-I
Published: October 2019

β-amyloid formation in the brain is one of the characteristics of Alzheimer’s disease. Exposure to this peptide may result in reentry into the cell cycle leading to cell death. The phytoestrogen equol has similar biological effects as estrogen without the side effects. This study investigated the possible mechanism of the neuron cell-protecting effect of equol during treatment with Aβ. SH-SY5Y neuroblastoma cells were treated with either 1 μM S-equol or 10 nM 17β-estradiol for 24 h prior to 1 μM Aβ (25–35) exposure. After 24 h exposure to Aβ (25–35), a significant reduction in cell survival and a reentry into the cell cycle process accompanied by increased levels of cyclin D1 were observed. The expressions of estrogen receptor alpha (ERα) and its coactivator, steroid receptor coactivator-1 (SRC-1), were also significantly downregulated by Aβ (25–35) in parallel with activated extracellular signal-regulated kinase (ERK)1/2. However, pretreatment of cells with S-equol or 17β-estradiol reversed these effects. Treatment with the ER antagonist, ICI-182,780 (1 μM), completely blocked the effects of S-equol and 17β-estradiol on cell viability, ERα, and ERK1/2 after Aβ (25–35) exposure. These data suggest that S-equol possesses a neuroprotective potential as it effectively antagonizes Aβ (25–35)-induced cell cytotoxicity and prevents cell cycle reentry in SH-SY5Y cells. The mechanism underlying S-equol neuroprotection might involve ERα-mediated pathways.
Association between Different Animal Protein Sources and Liver Status in Obese Subjects with Non-Alcoholic Fatty Liver Disease: Fatty Liver in Obesity (FLiO) Study
Authors: Recaredo Gregorio , Marin-Alejandre Bertha Araceli , Cantero Irene , Monreal J. Ignacio , Herrero José Ignacio , Benito-Boillos Alberto , Elorz Mariana , Tur Josep A. , Martínez J. Alfredo , Zulet M. Angeles , Abete Itziar
Published: October 2019

Non-alcoholic fatty liver disease (NAFLD) is considered the hepatic manifestation of metabolic syndrome. Obesity and unhealthy dietary habits are described as risk factors for NAFLD. The aim of this study was to investigate the association between the consumption of different animal protein sources and hepatic status in NAFLD adults. A total of 112 overweight/obese participants with NAFLD from Fatty Liver in Obesity (FLiO) study were evaluated at baseline. Diet, body composition, and biochemical variables were evaluated. Hepatic status was also assessed by Magnetic Resonance Imaging, ultrasonography, and elastography. Red meat consumption showed a positive relationship with liver iron content (r = 0.224; p = 0.021) and ferritin concentration (r = 0.196; p = 0.037). Processed meat consumption exhibited a positive association with liver iron content (r = 0.308; p = 0.001), which was also found in the quantile regression (β = 0.079; p = 0.028). Fish consumption was related with lower concentration of ferritin (r = −0.200; p = 0.034). This association was further evidenced in the regression model (β = −0.720; p = 0.033). These findings suggest that the consumption of different animal protein sources differentially impact on liver status in obese subjects with NAFLD, showing fish consumption as a healthier alternative for towards NAFLD features.
Obesity and 25(OH)D Serum Concentration Are More Important than Vitamin D Intake for Changes in Nutritional Status Indicators: A Population-Based Longitudinal Study in a State Capital City in Southern Brazil
Authors: Cembranel Francieli , d’Orsi Eleonora , Wagner Katia Jakovljevic Pudla , Giehl Marui Weber Corseuil , Moreno Yara Maria Franco , González-Chica David Alejandro
Published: October 2019

Our objective was to investigate the relationship between dietary vitamin D intake and serum concentration of vitamin D (25(OH)D) on changes in body weight, waist circumference (WC), and body mass index (BMI), and to determine if this relationship changes between obese and non-obese individuals at baseline and those who have or do not have 25(OH)D deficiency. This was a prospective study with a sample of 572 individuals aged 25–65 years, who were participants in the cohort study EpiFloripa Adults. Changes in weight (in kg), BMI, and WC between 2012 and 2014 were evaluated as outcomes. The main exposure was the dietary intake of vitamin D (2012), and the 25(OH)D serum concentration was secondary. When the analyses were stratified by the presence of obesity in the baseline, among obese individuals it was observed that those in the extreme categories of vitamin D intake had an average gain of 3.0 kg in weight, 0.9 kg/m2 in BMI, and 1.7–2.7 cm in WC. When 25(OH)D serum concentration were incorporated into the analyses, it was observed that non-obese subjects not having 25(OH)D deficiency had a mean reduction of 2.3 cm in WC. In conclusion, the increases in body weight, BMI, and WC were higher over time in obese patients with deficient 25(OH)D serum concentration, regardless of dietary vitamin D intake.