bluuebunny/crossref_metadata_2025 huggingface dataset:
AROPS: A Framework of Automated Reaction Optimization with Parallelized Scheduling
Authors: Ruan Yixiang , Mo Yiming , Lin Sen
Published: September 2022

With the development of automated experimental platforms and optimization algorithms, chemists can easily optimize chemical reactions in an automated and high-throughput fashion. However, the modules in existing automated experimental platforms are operated in a predefined way without orchestrating with the optimization algorithm, thus leaving room for further efficiency improvement. Here, we introduced a framework of automated reaction optimization with parallelized scheduling (AROPS) to realize the integration of optimization algorithm and module scheduling. AROPS relies on a customized Bayesian optimizer to solve multi-reactor/analyzer reaction optimization problems with three different scheduling modes to arrange tasks for various experimental modules. In addition, a mechanism based on probability of improvement (PI) for discarding unpromising on-going experiments was developed to facilitate freeing-up valuable experimental resources in parallelized optimization. We tested the performance of AROPS using a hardware emulator on three typical benchmark reactions encountered in organic synthesis, illustrating that AROPS can trade off optimization time and cost according to the chemists’ preference.
Chlorination of Hydrogenated Silicon Nanosheets Revealed by 1H{35Cl} and 29Si{35Cl} Solid-State NMR Spectroscopy
Authors: Dorn Rick , Ryan Bradley , Lamahewage Sujeewa , Dodson Mark , Biswas Rana , Panthani Mathew , Rossini Aaron
Published: September 2022

Two-dimensional silicon nanosheets (Si-NS) synthesized by topotactic deintercalation of CaSi2 are hypothesized to consist of buckled layers of sp3 hybridized silicon atoms that are bonded to three other framework Si atoms and a terminal atom or functional group such as H, Cl or an -OH group. Here, we apply 1H{35Cl} and 29Si{35Cl} Reso-nance-Echo Saturation-Pulse DOuble-Resonance (RESPDOR) solid-state NMR experiments to directly confirm the presence of chlorinated Si atoms within Si-NS. Plotting the observed 1H{35Cl} RESPDOR dephasing as a function of the 35Cl saturation pulse offset enables measurement of the 35Cl central-transition (CT) quadrupolar powder pattern and the chlorine quadrupolar coupling constant (CQ). Modelling the 1H{35Cl} RESPDOR dephasing curve shows that the Si-Si interlayer spacing is approximately 6 Å. Plane-wave DFT calculations suggest that the direct band gap transition of Si-NS decreases with increasing chlorination, suggesting that chlorination is a viable pathway to tune the band gap for applications.
Recyclable and Convenient-to-Handle Pt/Ethylene Glycol Catalytic System – an Approach to Sustainable Hydrosilylation
Authors: Goncharova Irina , Beletskaya Irina , Arzumanyan Ashot
Published: September 2022

This study presents a highly efficient and simple recyclable catalytic system for heterophase hydrosilylation. This catalytic system consisting of a commercially available platinum precatalyst, namely K2PtCl4, and a cheap green solvent, namely ethylene glycol (EG), is easily prepared by dissolving K2PtCl4 in EG without employing ligands, additives, or an inert atmosphere, at r.t. The suggested method allows up to 45 recycles with quantitative conversion under air at r.t. to be performed. The high reaction rate (from 2 to 20 min per cycle) and low catalyst load (0.001 – 0.1 mol%) allow high values of TON (up to ~ 104 – 105) and TOF (up to ~ 103 – 106) to be reached. This approach is applicable to a wide range of unsaturated compounds, such as functional and nonfunctional terminal or internal alkenes, alkynes, and alkyl-, phenyl-, and siloxy-containing hydride silanes. Moreover, the heterophase catalytic system is suitable for the synthesis of linear and cross-linked polyorganosiloxanes. In most cases, the reaction provides high yields (up to 95-99%) and selectivity. It gives mostly anti-Markovnikov products which can be isolated from the catalytic system by simple decantation. The process is scalable to gram quantities.
SIRT2i_Predictor: A machine learning-based tool to facilitate the discovery of novel SIRT2 inhibitors
Authors: Djokovic Nemanja , Rahnasto-Rilla Minna , Lougiakis Nikolaos , Lahtela-Kakkonen Maija , Nikolic Katarina
Published: September 2022

Selective sirtuin 2 (SIRT2) inhibitors hold therapeutic promise for treatment of wide range of age-related diseases. Despite promising preclinical results, none of the SIRT2 inhibitors have reached clinical trials. In order to facilitate development of novel SIRT2 inhibitors, a machine learning based tool titled SIRT2i_Predictor was developed through this work. The main utility of SIRT2i_Predictor is to support virtual screening (VS) campaigns and facilitate the selection of candidates for in vitro and in vivo evaluation. Appealing web-based interface which allows visualization of structure-activity relationships makes SIRT2i_Predictor a valuable tool in the lead optimization projects as well. The tool was built on panel of high-quality machine learning regression-based and binary classification-based models for prediction of inhibitors potency, as well as multiclass classification-based models for predictions of inhibitors SIRT1-3 isoform selectivity. The regression and classification structure-activity relationship models were created for 1797 publicly available compounds by exploring combinations of 5 machine learning algorithms and 4 molecular representations. SIRT2i_Predictor was demonstrated to be able to screen around 200000 compounds in matters of minutes with comparable chemical space coverage to the structure-based VS. The tool was applied in screening of in-house database of compounds further corroborating the utility in prioritization of compounds for costly in vitro screening campaigns. The code of SIRT2i_Predictor is made available at https://github.com/echonemanja/SIRT2i_Predictor.
Bioconjugation of Electron-Probe Au25 Nanocluster to Monoclonal Antibody
Authors: Malawska Katarzyna Joanna , Takano Shinjiro , Oisaki Kounosuke , Yanagisawa Haruaki , Kikkawa Masahide , Tsukuda Tatsuya , Kanai Motomu
Published: September 2022

We report the first bioconjugation of Au25 nanocluster to a monoclonal antibody without protein engineering, in a step toward the development of high-resolution probes for cryogenic electron microscopy (cryo-EM) and tomography (cryo-ET). To achieve this, we improved the tryptophan (Trp)-selective bioconjugation step by using easy-to-analyze hydroxylamine (ABNOH) reagents in a pH-neutral buffer, instead of using N-oxyl radicals (ABNO) under acidic conditions as previously developed. This new protocol allowed for the application of Trp-selective bioconjugation to acid-sensitive proteins such as antibodies. We found that a two-step procedure, utilizing first Trp-selective bioconjugation for homogeneous introduction of azide groups to the protein and then strain-promoted azide-alkyne cycloaddition (SPAAC) to attach bicyclononyne (BCN)-presenting, redox-sensitive Au25 nanocluster, was key to successful immunogold synthesis. This procedure is scalable. The covalent labeling of the antibody with gold nanoclusters was confirmed by various analytical methods, including cryo-EM analysis of the Au25 nanocluster conjugates. In comparison with a non-homogenous variant prepared by lysine-selective bio-conjugation, Trp-selective conjugates exhibited both satisfactory gold cluster modification and minimal loss of antigen-binding ability.
Development of a series of quinazoline-2,5-diamine derivatives as potent hematopoietic progenitor kinase 1 (HPK1) inhibitors
Authors: Shi Huanyu , Tang Haotian , Li Yan , Chen Danqi , Liu Tongchao , Chen Yuting , Wang Xin , Chen Lin , Wang Ying , Xie Hua , Xiong Bing
Published: September 2022

Hematopoietic progenitor kinase 1 (HPK1) is a serine/threonine kinase that serves as the negative regulator of multiple immune signaling pathways. Genetic studies using HPK1 knockout and kinase-dead mice suggested that inhibiting HPK1 either alone or in combination with immune checkpoint blockade could be promising strategy in cancer immunotherapy. Herein, we report the design, synthesis and structure–activity relationship (SAR) study of a series of potent HPK1 inhibitors bearing quinazoline-2,5-diamine scaffold. Three rounds of SAR explorations led to the identification of 9h, the most potent compound in this series which harbors a 2-methyl-1,2,3,4-tetrahydroisoquinolin-7-yl substituent. Further biological studies using human immune cells demonstrated that 9h could strongly inhibit the downstream phosphorylation, augment the IL-2 secretion and reverse the PGE2-induced immune suppression. Overall, 9h can serve as a tool compound to help with demonstrating the pharmacological role of HPK1 kinase inhibition, and our investigation provided a reliable reference for the later development of HPK1 inhibitors.
Thiamine Analogues as Tools to Study Pyruvate Dehydrogenase in Cancer: Biochemical, Computational and Cellular Analyses
Authors: Leeper Finian James , Chan Alex , Ho Terence , Parle Daniel
Published: September 2022

Pyruvate dehydrogenase complex (PDHc) suppression has long been regarded as a mechanism for cancer cells to manifest the well-known Warburg effect. However, in recent years, it has been proposed that whether PDHc is suppressed or activated in cancer development is context-dependent. The PDHc E1 subunit (PDH E1), a thiamine pyrophosphate (TPP)-dependent enzyme, catalyses the first and rate-limiting step of the complex so there is a need for selective PDH E1 inhibitors. Using thiamine/TPP analogues, we have developed potent and selective inhibitors of mammalian PDH E1. These inhibitors will aid work on understanding of the oncogenic role of PDHc and, ultimately, the development of anticancer strategies.
A Relativistic Particle Model of the H-Atom
Authors: Dunstan Dave
Published: September 2022

A relativistic classical particle model of the H-atom is presented. The balance of the electrostatic field and the spacetime field generates a quantized atom of singular radius. The radius is quantized due to the quantized nature of the electric charge. A particle model for the atom yields a quantized electron orbital that is consistent with quantum mechanics.
Exploring the Developmental Stages of Amharic Nominal and Verbal Gender: Evidence for processability theory
Authors:   Addis Ababa University, Ethiopia, H. Alemu , Ado D. ,   Addis Ababa University, Ethiopia
Published: September 2022

The purpose of this article is twofold. First, it explores the order of the development of nominal and verbal gender of Amharic, which is one of the Ethio-Semitic languages. Second, it provides empirical evidence for the typological plausibility of processability theory (PT). In fact, PT has been tested in typologically different languages (e.g., English, Italian, and Japan); however, it does not have any validation from Ethiopian languages in general and Ethio-Semitic languages in particular yet. Relevant data was collected from sixteen respondents via picture description tasks, short storytelling, interviews, story re-telling, and spot the difference tasks. Distributional analysis was conducted for the analysis, and the point of emergence of target structures was determined using the emergence criteria. Accordingly, the result shows that the development of gender assignment is compatible with processability theory’s predictions in that lexical procedure precedes phrasal procedure, which is followed by S-procedure. Moreover, the masculine gender emerged earlier than its feminine counterpart at all developmental stages. However, subject agreement markers in pro-drop context emerged at stage two preceding subject-verb agreement. This finding is against processability theory’s claim that suggests subject agreement markers only emerge at stage four of the processability hierarchy disregarding their stages of development in pro-drop context in particular.
A critique of the hypothesis that CA repeats are primary targets of neuronal MeCP2
Authors: Chhatbar Kashyap , Connelly John , Webb Shaun , Kriaucionis Skirmantas , Bird Adrian
Published: September 2022

The DNA-binding protein MeCP2 is reported to bind methylated cytosine in CG and CA motifs in genomic DNA, but it was recently proposed that arrays of tandemly repeated CA containing either methylated or hydroxymethylated cytosine are the primary targets for MeCP2 binding and function. Here we investigated the predictions of this hypothesis using a range of published datasets. We failed to detect enrichment of cytosine modification at genomic CA repeat arrays in mouse brain regions and found no evidence for preferential MeCP2 binding at CA repeats. Moreover, we did not observe a correlation between the CA repeat density near genes and their degree of transcriptional deregulation when MeCP2 was absent. Our results do not provide support for the hypothesis that CA repeats are key mediators of MeCP2 function. Instead, we found that CA repeats are subject to CAC methylation to a degree that is typical of the surrounding genome and contribute modestly to MeCP2-mediated modulation of gene expression in accordance with their content of this canonical target motif.
Geomembrane Filter Thread Technique’s Potential to Increase Salt Production in Jeneponto Regency
Authors: Ramly Zakia Asrifah , Ahmad Nur , Juhaseng Nur Asriyanti
Published: September 2022

The problem of salt production by Jeneponto Regency is influenced by the traditional way of making salt. The introduction of GFTT (Geomembrane Filter Thread Technique) as a salt-making technology is an effort to increase salt production in the Jeneponto district, both in quality and quantity. This activity was carried out in Biring Parang Jaya Village, Jeneponto Regency. Activities are carried out by applying threaded techniques to salt ponds, making filter devices on salt ponds, and installing geomembranes as salt tablets. Based on the application of technology, the salt produced by GFTT has better quality and quantity than traditional salt. Salt produced by GFTT is classified as high grade with 99.96% NaCl and 0.04% moisture content.
Enhancing replica management in a cloud environment using data mining based dynamic replication algorithm
Authors: Rambabu D.
Published: September 2022

Cloud computing has recently gained a lot of popularity and attention from the research community. One of the many on-demand services that large-scale applications provide to cloud customers is storage, which accumulates more generated data and subsequently leads to the need for storage. Despite the fact that users can use the cloud to store and provide the type of storage that desire, it still takes a significant amount of time to store and retrieve data due to the large accumulation of data. Due to the need to improve data availability, response time, reliability, and migration costs, the current storage engine needs to be replicated across multiple sites. When copies are properly distributed, data replication speeds up execution. The biggest challenges in data replication are choosing which data to replicate, where to put it, how to manage replication, and how many replicas it needs. Therefore, various studies have been carried out on some data mining-based data replication systems to evaluate replication issues and manage cloud storage. In most cases, data replication in a data mining environment is done using data mining along with a replication algorithm and a data grid policy. In addition, this paper addresses replica management issues and proposes affordable data replication in the cloud that satisfies all Quality of Service (QoS) requirements.
A study and interpretation of the distribution and kinematics of the 2001 Taupō Fault Belt seismic swarm using relative relocation techniques
Authors:   , McGregor Ross
Published: September 2022

The Taupō Volcanic Zone (TVZ) is the southern extent of the Tonga-Kermadec volcanic arc, forming a rifted arc due to its interaction with the nearby Hikurangi Subduction Zone. The TVZ aligns closely with the Taupō Rift, with the two structures accommodating extension in the region via magmatic and tectonic processes. Rates of seismicity are high in this area, especially around Taupō Volcano in the central TVZ. A swarm of earthquakes occurring between January and June 2001 contrasts with other periods of increased seismicity, occurring slightly north of the volcano within the Taupō Fault Belt. We have analysed this swarm of earthquakes to investigate potential interaction between the tectonic and magmatic systems at Taupō.

We obtained data from two temporary seismic arrays deployed at the time of the swarm and processed the data using non-linear location, matched-filter detection and differential relocation to yield over 2000 more earthquakes than initially detected by GeoNet. The relocated events indicated that earthquakes occurring within the fault belt formed vertical clusters near small, unidentified faults. Prior to seismicity within the Taupō Fault Belt, a large earthquake cluster was located beneath Lake Taupō’s Western Bay, previously thought to be aseismic. Focal mechanisms calculated for six of the eight largest magnitude earthquakes within the swarm are consistent with a rotation of the maximum compressive stress axes to horizontal during the swarm.

We interpret the earthquakes detected beneath the Western Bay as signalling unrest from an intruding, possibly mafic, magma body, such as have been identified at numerous other calderas. Fluids and increased horizontal stress from this intrusion triggered the resulting seismicity in the Taupō Fault Belt, which display vertical clustering and small-scale propagation to support this. Increased horizontal pressure from the intrusion rotated the axis of maximum compressive stress, indicated by strike-slip focal mechanisms and mirroring the same process in other caldera settings. This is a further example of volcano-tectonic interactions in the central TVZ, involving a previously unexplored area beneath Lake Taupō’s Western Bay.
Love on the Brain: The Impact of Sexual Arousal on Sexual Decision-making
Authors:   , Savio Joseph
Published: September 2022

Does sexual arousal predict riskier sexual decision-making? A study by Ariel and Lowenstein (2006) first investigated this relationship and found that sexual arousal significantly increased sexually risky and aggressive behaviour. The primary aim of our study was to replicate the findings of Ariel and Lowenstein (2006) using both male and female participants (N = 91). Our study also investigated the role of trait impulsivity and sexual decision-making, as well as examining the physiological and self-report concordance of arousal. Sexually explicit video clips as well as hypothetical romantic scenarios were used to evaluate the effects of sexual arousal on sexual risk-taking intentions. Additional anger, amusement and neutral conditions were used as comparison conditions. The findings from this study suggests that sexual arousal impacted sexual decision-making but to a lesser degree than what was found in Ariely & Loewenstein (2006). Impulsivity was also shown to be a significant factor within this relationship as individuals higher in trait impulsivity, self-reported riskier sexual decisions. Physiological and self-report measures showed no concurrency. Taken together, these findings suggest that in scenarios where both men and women are sexually aroused, they may lower their inhibition and experience impaired decision-making. This effect becomes further compounded if individuals are also high in trait impulsivity. This interaction may have a negative impact during sexual encounters and be a key contributor to the STI and sexual violence pandemic.
Analysis of mechanical properties of spatial truss structures sections
Authors: Zommer S. A. , Kravchunovsky A. P.
Published: September 2022

The paper presents the results of computational study of sections of spatial truss structures. They can form large supporting structures on spacecraft to place onboard equipment. The calculations were carried out by the finite element method in FEMAP with Nastran. The chosen section, the object of study, is a set of straight rods rigidly connected at the nodes in such a way that the cross section of the truss structure is a triangle. Calculation models, procedures for calculation and results analysis are presented. The purpose of the calculation is to determine how the relative position of the rods in the structural scheme affects the mechanical properties of the structure. The main criterion for strength estimating was the magnitude of the stresses derived by load. Stiffness was determined by the value of the first natural frequency. The sequential addition of rods and varying their connection allow modifying the structural schemes of truss structures. Next, the mechanical properties of the structure which effected by made modifications were evaluated again. Thus, six structural schemes of sections of the truss structure, obtained from the results of the study, have been developed. At the same time, the mass of the section, its shape and dimensions, the material and shape of the rods section as well as the initial and boundary conditions, remained unchanged. Based on the results of the analysis, someone can notice that each structure has unique mechanical characteristics. Thus, the paper gives recommendations for choosing a specific structural scheme of the section, depending on the required operating conditions and acceptable manufacturing technology. So, the criteria for choosing one of the above various sections of a truss structure or the principle of its building can be the complexity of manufacturing, maximum stiffness and strength, or minimum displacement. Structural schemes of sections with the highest strength and capability have been selected for use as part of spatial truss rods of spacecraft.
El racismo médico de salud en México: aproximación cultural e histórica
Authors: Mijangos Fuentes Karla Ivonne
Published: September 2022

El racismo más que una categoría diferencial es política, porque las instituciones que la validan se sirven de la misma, es decir, es a través del enclasamiento y la racialización de las corporalidades como se ha justificado la expropiación, conquista y colonialidad de tierras, el genocidio, el exilio, la participación política privilegiada y el epistemicidio de saberes e identidades. En general, el colonialismo se sirvió de diversos dispositivos, como la ciencia médica, que permitieron estructurar todo el arsenal neoliberal, porque a partir de la institucionalización de la universidad y del poder científico que le fue otorgado, esta contribuyó a estigmatizar los cuerpos en función de parámetros de blanquitud. Por tanto, estudiar la configuración de este pensamiento blanco como epistemología del conocimiento biomédico, nos permite comprender las matrices coloniales que atraviesan la construcción de la salud institucional, la normalidad de los cuerpos blanqueados y la deshumanización racial en y para la atención.
DIGITALIZATION OF HEALTHCARE: CURRENT ASPECTS AND WAYS OF FURTHER DEVELOPMENT
Authors: R.V. Kubasov , Y.A. Lenina , M.I. Mytsuk , E.D. Kubasova , V.V. Lupachev
Published: September 2022

Abstract. Efficient management of the health care system is essential to ensure the high quality of health care facilities, both public and private. The expected results of work to improve the efficiency of health care system involve the introduction of modern complex systems, among which digital technologies play a leading role. The value of digitalization will help healthcare organizations overcome the challenge of limiting the use of resources to meet growing healthcare needs. The digitalization of domestic healthcare, which covers electronic prescriptions, electronic referrals and the exchange of electronic medical records, has been implemented on the platform of the Integrated State Information System since 2018. According to forecasts, its implementation and maximum use should be completed and implemented to the maximum by 2025. The success of this implementation is closely related to the level of digital skills of both healthcare providers and users of healthcare services. It is believed that the widespread introduction of electronic technologies in healthcare will solve many current problems in this industry. However, at the present time its use is not yet very well developed, and its potential is little used. There are many reasons that promote or limit the spread of digital transformation in the healthcare sector. This is, first of all, knowledge, opportunities for learning, the ability to interact, systemic thinking. Good managerial responsibility is critical to transforming healthcare. Implementing eHealth requires new and innovative workflows. At the same time, both the medical staff and the administrative link must be aware that their work will change radically. It will evolve from a routine to an activity of learning, skill development and constant change in work practices.
ALGORITHM OF APPLICATION OF THE INDIVIDUAL – GROUP PROTOCOL IN THE COMPLEX REHABILITATION OF PATIENTS WITH SARCOPENIA
Authors: I.E. Pleshchev , V.N. Nikolenko , E.E. Achkasov , A.N. Shkrebko
Published: September 2022

Abstract. With the beginning of the aging of the population, diseases associated with old age and senility have become a problem for public health worldwide. This process is accompanied by certain physiological changes. Sarcopenia is one of the causes of these changes and is defined as a clinical syndrome associated with age-related loss of skeletal muscle mass, strength, function and physical performance. Sarcopenia is officially recognized as a muscular disorder with the diagnostic code ICD‐10‐CM (M62.84) and is associated with various adverse outcomes, including falls, impaired mobility, weakness and death.
The present study included 198 elderly male patients aged 61 to 75 years (average 68±3.27 years) suffering from sarcopenia. The patients were divided into groups I and II depending on the method of physical rehabilitation. Physical therapy classes were held for 12 weeks on the basis of the State Medical Institution of the Yaroslavl Regional Gerontological Center. The study participants voluntarily agreed to conduct a comprehensive medical and anthropological examination during 4 control sections, including antropometry, bioimpedance analysis, and hand dynamometry.
Based on the data obtained, a computer program "Program for assessing the quality of rehabilitation in patients with progressive and generalized loss of muscle mass and muscle strength" was created, and a protocol for the physical rehabilitation of elderly patients with sarcopenia was developed.
THE CURRENT SITUATION OF PERINATAL AND INFANT MORTALITY IN RUSSIA
Authors: A.A. Nurova , R.K. Mirzoeva , D.S. Berdysh
Published: September 2022

Abstract. Perinatal medicine has caused an improvement in the health of newborns, as well as a decrease in the perinatal mortality rate in all countries of the world. The level and structure of perinatal mortality is one of the main demographic indicators of the population. The problem of reducing the infant mortality rate always remains relevant. The birth rate is noticeably increasing every year. Due to the increase in the birth rate in the country, perinatal centers are being improved, which are able to provide qualified medical care quickly, efficiently and in a short time. The indicator of perinatal mortality depends on many factors: firstly, it is the health of parents, their living conditions and lifestyle. Secondly, the perinatal mortality rate depends on the socio-economic conditions of the population, the level of medical capacity, as well as the patient orientation of medical personnel. This article reveals the main causes of perinatal and infant mortality, shows the negative impact of system gaps in the treatment and care of pregnant women. The main factors of perinatal mortality are premature birth, intrauterine infections, congenital malformations, negligent attitude of pregnant women to their health and the health of their child. A significant part of unfavorable perinatal outcomes is determined by premature birth. One of the main reasons for under-bearing is the socio-economic conditions of the country. Thus, in economically different countries, the rate of premature birth ranges up to 5%, in African countries up to 15%. Maternal infections, hormonal disorders, extragenital diseases during pregnancy are also factors affecting premature birth. It turned out that intrauterine infections reach up to 70% among premature babies. According to autopsy results in 37.5%, intrauterine infection was the main cause of death. The provision of high-quality, timely and high-tech care, professional development of medical workers, modernization of medical equipment, constant monitoring of the course of pregnancy, early detection of abnormalities are priority areas that underlie the reduction of perinatal and infant mortality and stabilization of the demographic situation.
THE STUDY OF NECROGENIC ACTION OF 0,2% NORADRENALINE HYDROTARTRATE SOLUTION DURING ITS INTRAPROSTATIC INJECTION IN RATS
Authors: A.O. Lobkarev , R.Kh. Khafizyanova
Published: September 2022

Abstract. Background. Previously, we first formulated the concept of targeted prostatic pharmaconecrosis (TPPhN). TPPhN is the death of those areas of the prostate tissue, that are proven or suspected to contain tumor cells of prostate cancer, due to the direct injection of a pharmacologically active substance into the prostate tissue. The aim: to study whether TPPhN occurs in the prostate tissue of male rats three hours after injection of 0.05 ml noradrenaline hydrotartrate (NH) solution into it. Materials and methods. The experiments were carried out on 12 outbred male rats aged 10 months. The animals were divided into two groups of six individuals each. Under anesthesia, the rats of the first group received only the injection of 0.05 ml 0,2% solution of NH into the left lobe of the prostate. Rats of the second group first received doxazosin 0.00025 g intragastrically, and then an intraprostatic injection of 0.05 ml of a 0.2 % solution of NH into the left lobe of the prostate was made. 3 hours after intraprostatic injection, macroscopic changes in the left lobe of the prostate were assessed, then the left lobe was resected. Results and conclusions. In all experiments, a demarcation line was clearly visible, delimiting the focus of TPPhN from intact tissue of the prostate. Typical morphological changes in the prostate tissue in the focus of TPPhN were studied. Close attention was paid to the characteristics of the ischemic penumbra between the focus of TPPhN and intact prostate tissue. It was found that in animals of the second group, the penumbra turned out to be clearer and narrower; due to this, the focus of TPPhN was sufficiently sharply delimited from normal prostate tissue. The fact established by us allows us to consider such a combination of substances as promising in the development of the TPPhN technique in humans.
INVESTIGATIONS OF THE EFFECT OF IONIZING RADIATION ON PROTEIN SYNTHESIS IN LIVING ORGANISMS: ON THE 90TH ANNIVERSARY OF L.G. PROKOPENKO
Authors: S.A. Dolgareva , I.L. Brovkina , N.A. Nikishina , V.I. Puchkov
Published: September 2022

Abstract. The article is dedicated to the outstanding Kursk biochemist, Honored Scientist of the Russian Federation Leonid Georgievich Prokopenko. Brief biographical information is given, published scientific papers are analyzed, and his contribution to the study of one of the largest areas of research by biologists and chemists of the twentieth century - to the study of the effect of ionizing radiation on living organisms, on the synthesis of proteins that have the properties to bind to proteins synthesized by other organisms and inactivate them is determined. .
PHARMACOLOGICAL ACTIVITY OF LHT-17-19 IN CULTURES OF EGFR-EXPRESSING EPITHELIAL TUMORS
Authors: M.Yu. Kudryavtsev , O.N. Tumutolova , O.N. Deryabina , A.A. Epishkina , O.S. Vavilova , Yu.S. Gilevskaya , E.V. Blinova
Published: September 2022

Abstract. . In the study, the pharmacological activity of the pyridine derivative LHT-17-19 was studied in relation to the culture of pancreatic cancer cells PANC-1 and the culture of bladder cancer cells HT-29 expressing the epidermal growth factor receptor. The cultures were obtained from the library of tumor strains of the Moscow Research Institute of Oncology. P.A. Herzen - a separate structural subdivision of the Federal State Budgetary Institution "NMITs Radiology" of the Ministry of Health of Russia. LHT-17-19 was synthesized in the Department of Chemistry, Technology of Synthetic Medicines and Analytical Control of JSC VNTs BAV (Russia). LHT-17-19 and the reference drug "Doxorubicin" 50 mg each were added to the cultivation medium in the concentration range from 10-8 to 10-v. Compounds LHT-17-19 were shown to be able to induce death and suppress the growth of pancreatic cancer squamous culture (PANC-1) and bladder (HT-29) tumor cells expressing EGFR over a wide range of concentrations. So for pancreatic cancer (PANC-1), the effective inhibitory concentration for the compound LHT-17-19 was 1.3 × 10-7 M, while for the reference substance doxorubicin it was 6.0 × 10-5 M. bubble (NT-29) - 2.4 × 10-7 M and 5.2 × 10-4 M, respectively.
THE USE OF ATORVASTATIN IN MODERN CARDIOLOGY TO REDUCE THE RISK OF CORONARY HEART DISEASE
Authors: I.S. Avduev
Published: September 2022

Abstract. This article discusses issues regarding the use of drugs of the statin group for the prevention of diseases of the cardiovascular system, namely the use of atorvastatin in the prevention of coronary artery disease.
The study involved 80 patients. at the same time, 65 participants in the study suffered from coronary artery disease, they were diagnosed with angina pectoris of functional class 2, 15 people were potentially healthy, all participants had a common average age and gender characteristics.
All patients included in the study were divided into two groups: main and control. The first group received atorvastatin as therapy for IHD, the second group received standard IHD therapy, which included beta-blockers, antianginal drugs, etc. The results of the study were evaluated after 6 weeks and six months. An auxiliary instrumental method was the veloergometric test, as the most optimal option relative to others. This technique was used to verify the ischemic heart disease. To conduct a veloergometric test, the Cardiovit AT-10 apparatus was used, which allows you to gradually increase the load. The study included several stages, each stage assumed a 3-minute load. The load power was determined by a number of factors, such as weight, height of the patient, comorbidities, and age. In the first group, there is a clear positive trend and changes in the lipid profile, which persist for six months. In patients of the second group, such dynamics were not reliably detected. Also, all patients with coronary heart disease underwent a determination of the correspondence of biological age to chronological age. In this case, a formula based on the results of a well ergometric study was used.
IN VITRO EXPERIMENTAL STUDY OF TISSUE AND CELLULAR TOXICITY OF DRUGS
Authors: E.A. Dagar , Y.A. Mazov , E.V. Blinova , E.V. Semeleva , E.V. Eliseikina , N.V. Revina , S.P. Timoshkin , D.S. Blinov
Published: September 2022

Abstract. We studied the neurotoxic/neurotropic effects of a new derivative of dimethylacetamide and benzoic acid LHT-5-01 in a mixed neuroglial culture to determine both the effect of various concentrations of the compound on cell survival and their interaction with the glutamate noncainate receptor. To do this, a mixed neuroglial culture of the hippocampus of linear newborn (P 1-3) Sprague Dawley rats was resuspended in a neurobasal medium containing 0.5 mmol glutamine, 2% Supplement B27 and 15 μg/ml gentamicin. The cytoplasmic concentration of calcium in the ionized form [Ca2+]i was determined using a Fura-2 fluorescent probe (USA). The viability of culture cells—neurons and glial cells—was determined by the immunocytochemical method. Experimental studies were performed on cultures aged from 7 to 9 days, to which the test compound of dimethylacetamide LHT-5-01 was added at various concentrations in the laboratory of intracellular signaling of the Institute of Cell Biophysics of the Russian Academy of Sciences - a separate division of the Federal State Budgetary Scientific Institution Federal Research Center "Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences" (Pushchino) in strict accordance with the literary descriptions. Incubation of the culture with 10 μM of the LHT-5-01 compound completely suppresses the first phase of receptor activity, but does not prevent the formation of the second phase of NMDA receptor excitation. The established activity can be considered in the aspect of the prevention of glutamate-mediated excitotoxicity of the test compound.
THE EFFECT OF DECREASING THE TOXICITY OF FLUOROQUINOLONES IN DISSOLUTION MEDIA OF DIFFERENT COMPOSITION
Authors: I.V. Kazimova , A.L. Tatarinova , E.V. Uspenskaya
Published: September 2022

Abstract. The purpose of this work is to evaluate the effectiveness of reducing the toxicity of fluoroquinlone (FTQ) drugs. For this the Spirotox original method was used in combination with the model scheme of the enzymatic kinetics of Michaelis-Menten. Dissolution media of different nature and isotopic composition are considered as factors contributing to the reduction of the FTQ toxicity. The nature of a solvent with a different D/H ratio demonstrates the development of the kinetic isotope effect (KIE) during the course of biochemical processes ("light" water), as well as detoxifying properties (a complex of humic and fulvic acids, HFA). By the method of tangents to the graph of the“tL,sec – -lgC” function, an intersection with the ox axis corresponding to Ctox, mol·L-1 was found at the approximation point. It has been shown that the FTQ dilution with "light" water (with a reduced deuterium content, ≤ 1 ppm) or a complex of humic and fulvic acids contributes to an increase in the cells lifetime by 3-4 times compared with purified water. When selecting the highest value of the Pearson coefficient from the graphical dependence "tL – 1/Ln", as a result of extrapolation to x=0 and y=0, the enzymatic kinetic parameters were determined – Keq (by the negative segment on the ox axis) and fm (by the segment on the oy axis). The obtained values were for levofloxacin drug: Keq =3.99 ·10-2 mol·L-1, fm= 2.17 ·10-3 s-1 (dilution with purified water), Keq=1.12·10-1 mol·L-1, fm=4.62·10-2 s-1 (dilution with "light" water), Kp=6.37·10-1 mol·L-1, fm=1.61·10-2 s-1 (dilutions with HFA); for moxifloxacin drug: Keq=3.87·10-3 mol·L-1, fm= 7.87 ·10-2 s-1 (dilution with purified water), Keq=7.50·10-3 mol·L-1, fm=1.75·10-2 s-1 (dilution with "light" water), Keq=9.89·10-8 mol·L-1, fm=2.06·10-2 s-1 (dilution with GFC). The results obtained can be used in the pharmaceutical industry for the manufacture of medicines with an improved pharmacological profile.
PHYSIOTHERAPEUTIC METHODS IN THE COMPLEX TREATMENT OF LICHEN PLANUS OF THE MOUTH MUCOSA
Authors: E.G. Borisova , A.V. Pototskaya , A.M. Kovalevsky
Published: September 2022

Abstract. Over the past five years, the number of patients has increased, especially after suffering a coronavirus infection COVID-19, with manifestations of lichen planus on the oral mucosa. Objective. Improving the effectiveness of complex therapy of lichen planus of the oral mucosa with the use of physiotherapeutic methods of treatment. Materials and methods. Under supervision in the clinic of the Department of General Dentistry of the Military Medical Academy n. a. SM Kirov, there were 26 patients, aged 35 to 62 years, 19 women and 7 men, with a diagnosis of Lichen planus, erosive-ulcerative form. All patients with lichen planus underwent a general clinical dental examination, and the dynamics of reparative processes was monitored. Treatment of all patients began with the sanitation of the oral cavity, which included filling carious cavities, extraction of teeth in order to eliminate foci of chronic infection, and the exclusion of traumatic factors. If necessary, patients were recommended rational prosthetics. Patients were divided into 2 groups, depending on the local treatment. By age, sex and forms of the disease, the control and working groups were comparable. The first group - 12 people (46.15%) were patients who received only drug treatment (general and local). In the second group - 14 people (53.85%), there were patients who were included in the treatment complex sessions with diode laser radiation of red light with a wavelength of 665 ± 15 nm, a frequency of 80 ± 5 Hz for 10 days, every other day (5 procedures). Also, every other day (5 procedures) sessions of ultraphonophoresis were performed on the elements of the lesion with 0.5% hydrocortisone ointment. Results and discussion. Papules epithelialization processes were monitored on the 7th, 10th and 20th days, pain sensations were assessed on a visual analogue scale. The effect of the treatment in the first group was significantly lower than in the second group. Conclusion. Treatment of lichen planus should be complex, including physical factors, and individual, with the involvement of specialists from other profiles.
SPECIFICS OF EXTRACTS DISPERSION AND TRACE ELEMENT COMPOSITION DETERMINATION IN PLANT RAW MATERIALS OF LYCIUM BARBARIM
Authors: B. Tupa , E.V. Uspenskaya
Published: September 2022

Abstract. This paper presents the original study results of the fruits elemental composition and the infusions dispersed characteristics of Lycium barbarum L. in order to develop approaches to the standardization of promising plant materials. The Lycium belongs to the flowering division, class – dicotyledonous, family – Solanaceae (Solanaceae), tribe – Lycieae. The Lycium fruits – red oblong-ovate berries, 8-18 mm. To determine the weight loss from drying (11.5%), the fruits were dried for 4 hours at 60 °C in a BINDER FD oven. The dried fruits were analyzed by X-ray (XFA) for the content of chemical elements. The elements K, Cl, P, Ca, S, Fe, Cu, Zn, Br were found, with the K high values content (C,%). The fruits were also used to prepare water extracts, the holding time of which in a boiling water bath was 15, 30, 60 and 90 minutes. Dispersed characteristics (hydrodynamic radius d, nm, zeta potential ξ, mV, dimensional spectra of sub- and supramicron particles) in extracts were studied by DLS and LALLS methods. To remove the microns range particles from extracts there were used an inert membrane filter (Millex GV, d of pores = 0.22 m). The average particle size varies from 254 nm to 557 nm depending on the time of infusion and filtration. The 15 minutes infusion showed the smallest average particle size, the 90 minutes infusion - the largest ones. The infusion sizes decrease by about 2 times when passing through Millex filter. All infusions have a polydispersity index (PDI) value of less than 0.6 and are characterized by colloidal stability (zeta potential from -23 to -27 mV). The LALLS method demonstrated the presence of 50 m particles, the content of which decreases after passing through 0.22 m system. The proposed methods of analysis make it possible to standardize promising plant raw materials for pharmaceutical use.
ANGIOFIBROMA OF THE FOOT (CASE REPORT)
Authors: N.A. Koryshkov , I.I. Litvinov , V.V. Savgachev
Published: September 2022

Abstract. Relevance. Angiofibroma (angiomyofibroblastoma) is a rare soft tissue benign tumor. There are no descriptions of foot angiofibrom observations in the Russian specialized literature, which makes publications of this kind of materials relevant.
The aim of the study was to analyze the experience of treatment of large locally advanced angiofibroma of the foot.
Material and methods of research. A 17-year-old patient, according to ultrasound scanning and MRI, had a significant soft-tissue encapsulated tumor of the right foot. On 06.09.2018, a tumor removal operation was performed from three anatomical approaches - medial, posterior and plantolateral - with the maximum possible compliance with the principles of oncosurgery. Results and discussion. There were no complications, the wound healed by primary tension. Conclusion of histological examination of the extracted fragments of the tumor – angiofibroma of the foot. There is no data on tumor recurrence at the moment – more than three years after surgery.
Conclusion. Summing up the data of various authors and our own experience, it can be argued that angiofibroma (angiomyofibroblastoma) is a benign slowly expansively growing tumor, more often localized in the area of the external genitalia mainly in women, but also affecting the foot. An exhaustive preoperative examination using ultrasound scanning and MRI, careful preoperative planning based on the principles of oncosurgery, taking into account the complex topographic and anatomical features of the foot and the full implementation of this plan in a specialized hospital allows you to avoid complications and achieve a long relapse-free period in the surgical treatment of large locally advanced angiofibroma of the foot.
PROSPECTS FOR THE USE OF SPRAY FILM-FORMING SYSTEMS IN STOMA FIXATION
Authors: M.M. Shumkova , E.O. Bakhrushina , N.B. Demina
Published: September 2022

Abstract. The potential of spray film-forming systems for prevention and therapy of peristomal complications is highly significant. The advantages of the dosage form include creation of protective skin barrier, prolonged release of active substances of anti-inflammatory, antiseptic, regenerative spectrum of action, etc. Polymeric compositions can delay and partially prevent the aggressive media impact released by stomas via osmotic action and neutralize the allergic potential of adhesives for attachment of stoma and pouching system. A flexible and thin film can be created via controlled spraying that follows the skin texture, interrupting the irritation "vicious circle". The pH of the composition will reduce the pH of the irritated skin and related microbial contamination. Creation of such form will significantly improve stoma patients’ life quality, prevent some peristomal complications and facilitate skin care surrounding stoma, especially in difficult postoperative periods. The aim was to establish the potential of spray film-forming systems and the prospects of drug development in such a new dosage form to prevent peristomal complications as maceration, allergic dermatitis, and infection complications of mechanical injuries of bacterial and fungal nature, to determine the main properties required from the resulting film, as well as relevant active substances spectrum. In addition, two types of medications were suggested to help patients with self-diagnosis of stoma discharge on the surrounding skin and partially prevent irritation until the problem is detected.