bluuebunny/crossref_metadata_2025 huggingface dataset:
Pengaruh Penghasilan, Paritas, dan Kekhawatiran Tertular COVID-19 terhadap Kepatuhan Antenatal Care
Authors: Karim Hanifah , Melinawati Eriana , Aryoseto Lukman , - Darto
Published: September 2022

Pendahuluan: Antenatal Care (ANC) adalah pemeriksaan kehamilan yang dapat dilakukan oleh tenaga kesehatan di fasilitas kesehatan. Ibu hamil yang tidak melakukan ANC selama kehamilan, risiko terjadinya komplikasi menjadi lebih besar. Pada masa pandemi COVID-19 ini, kepatuhan ANC dapat dipengaruhi oleh beberapa hal, diantaranya adalah penghasilan, paritas, dan kekhawatiran tertular COVID-19 saat melakukan ANC. Penelitian ini bertujuan untuk menganalisis pengaruh penghasilan, paritas, dan kekhawatiran tertular COVID-19 terhadap kepatuhan ANC di Puskesmas Cawas 1, Kabupaten Klaten.
Metode: Penelitian ini merupakan penelitian observasional analitik dengan pendekatan cross-sectional. Penelitian dilaksanakan di Puskesmas Cawas 1, Kabupaten Klaten pada bulan November sampai dengan Desember 2021. Subjek penelitian berjumlah 63 orang yang memenuhi kriteria penelitian. Selanjutnya data dianalisis dengan uji Chi-Square.
Hasil: Setelah dilakukan analisis data, diperoleh hasil bahwa tidak ada pengaruh penghasilan (p-value= 0.101), paritas (p-value= 0.634), dan kekhawatiran tertular COVID-19 (p-value= 0.155) terhadap kepatuhan ANC pada masa pandemi COVID-19 di Puskesmas Cawas 1, Kabupaten Klaten
Kesimpulan: Tidak ada pengaruh penghasilan, paritas, dan kekhawatiran tertular COVID-19 terhadap kepatuhan ANC pada masa pandemi COVID-19 di Puskesmas Cawas 1, Kabupaten Klaten
Kata Kunci: Antenatal Care; Penghasilan; Paritas; COVID-19.
Tingkat Pemahaman Mengenai COVID-19 dan Kepatuhan Pemeriksaan Kehamilan (ANC) : A Cross Sectional
Authors: Larasati Raden Rara Pandhan Budi , Laqif Abdurahman , Melinawati Eriana , Aryoseto Lukman
Published: September 2022

Pendahuluan: Ante natal care (ANC) dilakukan untuk mengetahui perkembangan janin dan kesehatan ibu hamil. Ketidakpatuhan memenuhi jadwal pemeriksaan ANC  meningkatkan risiko terjadinya komplikasi kehamilan dan persalinan, Angka Kematian Ibu (AKI), dan Angka Kematian Bayi (AKB). Protokol kesehatan (prokes) di masa pandemi COVID-19 memberlakukan stay at home (tetap tinggal di rumah) dan social distancing (menjaga jarak). Ibu hamil merupakan individu yang rentan terhadap infeksi COVID-19. Keterbaharuan penelitian melihat pengaruh pengetahuan ibu hamil terhadap kepatuhan melakukan pemeriksaan ANC. Tujuan untuk mengetahui pengaruh tingkat pengetahuan mengenai COVID-19 terhadap kepatuhan subjek melakukan pemeriksaan ANC di masa pandemi COVID-19.

Metode: Design penelitian adalah cross-sectional study dengan wawancara pada ibu hamil yang datang ANC saat masa pandemi COVID-19 di Puskesmas Cawas 1, Klaten yang memenuhi kriteria inklusi dan eksklusi. Kelengkapan data diperoleh dengan pengisian kuesioner yang telah divalidasi dan catatan dari buku KIA. Olah data univariat dan bivariat dengan menggunakan uji Chi-Square.

Hasil: Jumlah responden sebanyak 66 orang dengan 77,3 % (51/66) berusia antara 20-35 tahun,  75,8% (50/66) berpendidikan tinggi, dan 69,7% (46/66) merupakan ibu yang tidak bekerja. Tidak terdapat pengaruh tingkat pengetahuan mengenai COVID-19 terhadap kepatuhan pemeriksaan kehamilan (ANC) dalam masa pandemi di Puskesmas Cawas 1, Klaten, dengan p value 0,673.

Kesimpulan: Tidak ada pengaruh tingkat pengetahuan COVID-19 terhadap kepatuhan ANC di masa pandemi di Puskesmas Cawas 1, Klaten.
Use of dispersion analysis in building a model of factor influence on the result of remote thermal control of biological objects
Authors: Hryhorenko Ihor , Hryhorenko Svіtlana , Zhuk Oleksandr
Published: September 2022

The solution to the scientific and practical problem of using dispersion analysis to build a model of factor influence on the result of remote thermal control of biological objects is considered. A model of the effect on the result of measuring the temperature of a biological object of four factors that negatively affect the accuracy of temperature measurement is presented, namely: air temperature, air dustiness, global radiation brightness, air humidity. The scheme of action of the specified factors is considered. In practice, it is not always possible to measure the temperature of a person or an animal by means of non-contact control by choosing a place in advance in which the influence of factors that negatively affect the accuracy of temperature measurement is not manifested during the measurement process. Therefore, it is necessary to develop a factor influence model that will take into account the most important factors that negatively affect the measurement result. This can be done using variance analysis. It was concluded that the obtained results enable further improvement of the methods of processing the results of non-contact temperature measurement of biological objects. Prospects for further research of the developed model are presented, namely, that it is possible to obtain an equation for evaluating the reliability of statistical conclusions about the informational significance of temperature control indicators; obtaining analytical ratios that will make it possible to estimate the amount of information for each of the temperature control indicators under the factorial influence on the transformation function of these indicators in order to rank the temperature control indicators according to the decrease in their sensitivity to changes in the temperature control parameter levels.
EXPERIMENTAL UNIT FOR DETERMINING BODY WEIGHT OF ASTRONAUTS AND LIGHT-WEIGHT OBJECTS IN ZERO-GRAVITY CONDITIONS
Authors: Kolomiitsev Oleksii , Komarov Volodymyr , Dmitriiev Oleh , Pustovarov Volodymyr , Oliinyk Ruslan
Published: September 2022

The transition of an astronaut into zero gravity leads to a certain restructuring of the body, including the redistribution of fluid flows in it. The process of dehydration of the body is stimulated, the astronaut loses weight. Knowledge of the regularities of changes in an astronaut's body weight, keeping it within normal limits thanks to a rational load distribution, rest, physical exercises, and a well-thought-out diet are extremely necessary in order to provide the astronaut with the most favorable conditions for adaptation in zero gravity, as well as to better prepare him for his return to Earth. Returning materials for scientific research and experiments, as well as equipment, from the space station to Earth also requires high-precision determination of the low weight of objects. The object of the research is an experimental unit that allows measuring the weight (parameters) of the astronaut’s body and the small weight of objects (equipment, devices, etc.) that oscillate. The subject of research is the frequency of natural oscillations of the dynamic system. The purpose of scientific work is the development of an experimental unit for determining the weight of the astronaut’s body and the low weight of objects in zero gravity. Conclusions. An experimental setup for determining the body weight of astronauts and the low weight of objects (control objects – СО) in zero gravity is proposed. For high-precision determination of the weight of the СО in zero gravity, the natural frequency (NF) was chosen as a diagnostic parameter. In the experimental installation, the weight of the OC is determined by changing the NF of the dynamic system "control object – moving anchor" depending on the attached weight of the СO. The essence of the work of the experimental unit is revealed, and its general appearance and structural scheme are presented.
Development of E Module Based on Android in Basic Graphics Design Course
Authors: Subastian Moh Dycki , Astuti Cindy Cahyaning
Published: September 2022

This study aims to development and feasibility of e modul based on Android  in the basic subjects of graphic design for class X Multimedia at SMK PGRI 2 Sidoarjo. This research was conducted at SMK PGRI 2 Sidoarjo with research subjects in class X Multimedia who received Basic Graphic Design subjects. In this study are research and development method of R and D uses the ADDIE model which consists of analysis, design, development, implementation, and evaluation. However, in this study, only three stages were applied, namely the analysis, design, development stages, while the implementation dissemination and evaluation stages were not carried out due to the limited time of the study and the impact of the Covid 19 outbreak. The android-based e-module has met the validity criteria by obtaining an average score from all aspects of the assessment, the media expert validator is 88 percent which is in the category suitable for use with revisions, and the material expert validator is 74 percent in the category suitable for use with revisions so that based on the assessment experts have been appropriate and suitable to be used as learning media in Basic Graphic Design subjects
Les bibliothèques publiques des communautés de langue officielle en situation minoritaire : enjeux actuels sur le développement des collections en français
Authors: Lachaîne Catherine , Shaughnessy Sarah , Carrier Hélène , Gareau-Brennan Céline , Caidi Nadia
Published: September 2022

Les bibliothèques publiques canadiennes jouent un rôle important dans le soutien des communautés de langue officielle en situation minoritaire, tant sur les plans culturel et socio-économique que linguistique. Pourtant, il existe relativement peu de recherches sur les pratiques et l’impact des bibliothèques en contexte linguistique minoritaire. Cette enquête exploratoire présente les résultats d’un sondage pancanadien sur les collections en français des bibliothèques publiques desservant les communautés francophones minoritaires au Canada, en mettant l'accent sur les pratiques du personnel des bibliothèques et leur niveau de satisfaction en ce qui concerne le développement, la promotion et l'évaluation de ces collections. L’analyse des résultats du sondage révèle quatre principaux facteurs, souvent interreliés, qui influencent le développement des collections dans les communautés francophones en situation minoritaire : le niveau de connaissance du français chez les employés, le recours à des fournisseurs et à des intermédiaires, le manque de ressources et de soutien, et le confinement du développement des collections aux contenus pour enfants et aux publics d'âge scolaire. Les résultats de cette étude sont partagés afin de susciter des discussions et une réflexion sur les défis uniques de ce travail.
Éditorial - La recherche en français en sciences de l'information au Canada et Partnership
Authors: Gamache Éthel
Published: September 2022

Partnership vise à encourager la dissémination de la production scientifique francophone et anglophone canadienne en bibliothéconomie et en sciences de l’information. Ce numéro met de l’avant la recherche en français avec trois articles. Ces manuscrits de qualité touchent différentes questions et furent un plaisir à lire et éditer. Mais, pour un numéro dédié à la recherche diffusée en français, pourquoi y a-t-il si peu d’articles?
Nonlinear Analysis by Applying Best Approximation Method in p-Vector Spaces
Authors: YUAN George X.
Published: September 2022

It is known that the class of p-vector spaces (0 < p ≤ 1) is an important generalization of usual norm spaceswith rich topological and geometrical structure, but the most tools and general principles with nature in nonlinearity have not been developed yet, the goal of this paper is to develop some useful tools in nonlinear analysisby applying the best approximation approach for the classes of 1-set contractive set-valued mappings in p-vectorspaces. In particular, we first develop the general fixed point theorems of condensing mappings which provideanswer to Schauder conjecture in 1930’s in the affirmative way under the setting of p-vector spaces. Then one bestapproximation result for upper semi-continuous and 1-set contractive set-valued is established, which is used as auseful tool to establish fixed points of non-self set-valued mappings with either inward or outward set conditions,and related various boundary conditions.
AMS Classification: 47H04, 47H10, 46A16, 46A55, 49J27, 49J35, 52A07, 54C60, 54H25, 55M20
Factors Associated with The Speed and Scope of Diffusion of COVID-19 Therapeutics in a Nationwide Healthcare Setting: A Mixed Methods Investigation
Authors: La Jennifer , Fillmore Nathanael R , Do Nhan V , Brophy Mary , Monach Paul A , Branch-Elliman Westyn
Published: September 2022

Background:
The global COVID-19 pandemic is an opportunity to evaluate factors associated with high levels of adoption of different therapeutics in a real-world setting. The aim of this nationwide, retrospective cohort study was to evaluate the diffusion and adoption of novel therapeutics with an emerging evidence basis and to identify factors that influenced physicians’ treatment decisions.
Methods:
Cohort Creation: A cohort of Veteran patients with a microbiologically-confirmed diagnosis of SARS-CoV2 was identified and cases were classified by disease severity (outpatient, inpatient with mild and severe disease, ICU). After classification of disease severity, proportion of cases (outpatients) and admissions (inpatients) in each category receiving each type of medication were plotted as a function of time.
Identification of Milestones and Guidance Changes: Key medications used for the management of COVID-19 milestones in the release of primary research results in various forms (e.g., via press release, pre-print, or publication in a traditional medical journal), policy events, and dates of key guidelines, were identified plotted as a timeline.
After a timeline was created, timepoints were compared to changes in medication use, and factors potentially impacting the magnitude (i.e., proportion of patients who received the treatment) and the speed (i.e., the slope of the change in use) of practice changes were evaluated.
Results:
Dexamethasone and remdesivir, the first two medications with clinical trials data to support their use underwent the most rapid, complete, and sustained diffusion and adoption; the majority of practice changes occurred after press releases and pre-prints were available and prior to guideline changes, although some additional uptake occurred following guideline updates. Medications that were not “first in class,” were identified later in the pandemic, and that had higher perceived risk had slower and less complete uptake regardless of the strength and quality of the evidence supporting the intervention.
Conclusions:
Our findings suggest that traditional and social media platforms and pre-print releases were major catalysts of practice change, particularly prior to the identification of effective treatments. The “first available treatment in class” impact appeared to be the single most important factor determining the speed and spread of diffusion.
Clinical Trial Registration:
Not applicable
Comparison of the effects of esketamine, sufentanil, or lidocaine combined with propofol on tussis reflection during upper gastrointestinal endoscopy: study protocol for a randomised, two centre, three-blind, controlled trial
Authors: Hou Haijun , Liu Lu , Xue Fushan , Tian Ming
Published: September 2022

Background: The endoscopic technique is the most widely utilised method for the diagnosis and treatment of upper gastrointestinal (GI) diseases. Tussis, which increases the incidence of airway spasm, aspiration, nausea, and vomiting, is a common complication faced during upper GI endoscopy. However, sedatives and analgesics exhibit inhibitory actions against airway reflexes to different degrees. Our assumption is that a combination of propofol and small doses of sufentanil, esketamine, or lidocaine, especially the combination of propofol and esketamine, might reduce tussis incidence.
Method: The study will be performed as a randomized controlled three-blind, two-centre trial. Patients undergoing upper GI endoscopy, ≥ 18 years old, with American Society of Anesthesiologists (ASA) classification I–III will be randomized to four groups: P group (single administration of propofol), P + S group (administration of propofol and sufentanil in combination), P + K group (administration of propofol and esketamine in combination), and P + L group (administration of propofol and lidocaine in combination) (N = 100 per group). The primary outcome, reflecting effectiveness of sedation, The primary endpoints include the frequency of tussis, nausea and vomiting, and/or body movements observed at the insertion of the endoscope into the pharyngeal cavity or within 5 min of endoscope insertion. Secondary outcome parameters are recovery assessment, patients’ and endoscopists’ satisfaction with the procedure, MMSE scores, MET scores, and sleep condition and the number of sedation-related events. Data on sedation-related events are collected by recording of vital signs, including oxygen saturation (SpO2), respiratory rate (RR), heart rate (HR), arrhythmias (electrocardiogram (ECG)), and non-invasive blood pressure (NIBP). Satisfaction parameters and mental states are collected by means of questionnaires and evaluation scales before and after the procedure and on different following days.
Discussion: Esketamine can reduce tussis occurrence with good tolerability and relax the bronchus, also provides high clearance rates and low possibility of adverse reactions. Unfortunately esketamine is also known for its psychotomimetic effects. We aim to demonstrate that the combination of esketamine with propofol for sedation in patients subjected to upper GI procedure is nevertheless superior to only administration of propofol or a combination of propofol with other anesthetics, such as opioids or lidocaine.
Trial registration: ClinicalTrials.gov. NCT05497492, Registered 09 August 2022. http://www.ClinicalTrials.gov/NCT0549749
Population WGS-based Spinal Muscular Atrophy Carrier Screening in a cohort of 1076 healthy Polish individuals
Authors: Sypniewski Mateusz , Kresa Dominika , Dobosz Paula , Topolski Piotr , Kotuła Lidia , Sztromwasser Paweł , Mroczek Magdalena
Published: September 2022

Spinal Muscular Atrophy is a severe neuromuscular disorder with an autosomal recessive inheritance pattern. The disease-causing gene is SMN1 and its paralogue, SMN2, is a disease course modifier. Both genes SMN1 and SMN2 show over 99.9% sequence identity and a high rate of crossing over in the genomic region. Due to this reason, SMN1/SMN2 is usually excluded from the whole genome sequencing (WGS) analysis and investigated with traditional methods, such as MLPA and qPCR. Recently, novel bioinformatic algorithms dedicated to analyzing this particular genomic region have been developed. Here, we analyze the SMN1/SMN2genomic region with a dedicated program, SMNCopyNumberCaller. We report a similar prevalence of SMN1 gene deletion carrier status (1 per 41 people) to published data from the Polish population (1 per 35 people). Additionally, SMNCopyNumberCaller can identify SMN2 CNVs and SMN2Δ7-8 present in 153 healthy Polish individuals. Two other programs for the CNV analysis in standard genomic regions were not able to give reliable results. Using WGS-based tools for SMN1/2genomic region analysis is not only an efficient method in terms of time, but will also enable more complex analysis such as silent carrier screening and identification of further genetic modifiers. Although still an experimental method, soon WGS-based SMN1/SMN2 carrier identification may become a standard method for the patients screened with WGS for other purposes.
Development of an Image Classification Pipeline for Atherosclerotic Plaques Assessment using Supervised Machine Learning
Authors: Kunchur Natasha N. , Mostaço-Guidolin Leila B.
Published: September 2022

Background: Atherosclerosis, an underlying cause of cardiovascular diseases, is achronic inflammatory condition resulting in the accumulation of plaque composedof lipids and other bio compounds within artery walls. Thus, narrowing thearterial lumen and leading to the blockage of blood flow, and rupture of thearteries. Atherosclerosis is known to be an inevitably progressive disease, resultingin an increase in inflammation and lipid accumulation with age. Through thecombination of coherent anti-stokes Raman Scattering (CARS) microscopy, anon-linear optical microscopy modality, and an automated pipeline for plaqueclassification; the stages of plaque progression can be assessed in a label-freemanner. The pipeline provides a basis for recognizing changes in the progressionand/or stabilization of atherosclerotic lesions.
Results: The use of machine learning in microscopy has been increasinglyallowing the classification of large amounts of images based on specific featuresrelevant to different applications. Based on a set of label-free CARS images ofatherosclerotic plaques (i.e. foam cell clusters) from a rabbit model, wedeveloped an automated pipeline to classify lesions based on their majormorphological features. Through the combination of image preprocessing andsegmentation, feature extraction and supervised machine learning algorithms, theclassification pipeline showcased the ability to exploit relevant plaquemorphological features to accurately classify 3 pre-defined stages ofatherosclerosis: early fatty streak development (EFS), early fibroatheroma (EF)and advancing atheroma (AA), greater than 85% class accuracy. Conclusions: Minute changes in the morphology of plaque can often beoverlooked. Through the combination of CARS microscopy and computationalmethods, a powerful classification tool was developed to identify the progressionof atherosclerotic plaque in an automated manner. The ability to differentiateamongst EFS, EF and AA present the opportunity to classify the onset ofatherosclerosis at an earlier stage of development, as well as greatly improvingthe potential of tracking effectiveness of novel therapeutic interventions
The lncRNA Snhg11 is required for synaptic function, neurogenesis and memory and is downregulated in the dentate gyrus of Down syndrome mouse models
Authors: Sierra Cesar , Sabariego Miguel , Fernández-Blanco Alvaro , Dierssen Mara
Published: September 2022

Down syndrome (DS) is the most common genetic cause of intellectual disability. Even though advances in the last decades have allowed better delineation of its pathogenetic mechanisms, its cellular and molecular bases are still poorly understood. Here, single-nuclei transcriptional profiles of the hippocampus of a DS mouse model revealed that the trisomy results in a highly cell-type specific alteration of the transcriptome. Strikingly, we observe a major transcriptomic shift in trisomic granule cells from the dentate gyrus (DG), without important changes in other hippocampal regions. We identified the small nucleolar RNA host gene 11, Snhg11, specifically downregulated in the trisomic DG, as the main contributor to the observed shift. Knockdown of Snhg11 in the DG of wild-type mice impairs synaptic function and adult neurogenesis and recapitulates trisomic hippocampal-dependent cognitive phenotypes. Our work highlights the importance of this long non-coding RNA in intellectual disability and reveals its functional involvement in the DG. These findings suggest the DG as a main region for the memory deficits observed in DS and indicate the relevance of new players such as long non-coding RNAs in its etiology.
From Evidence to Practice: Early Integration of Palliative Care in a Comprehensive Cancer Center
Authors: Lakhani Shamsah , Scalzitti David A. , Padrone Laura , Welch Diana Martins
Published: September 2022

Purpose
The American Society of Clinical Oncology (ASCO) recommends that all patients with a diagnosis of advanced cancer be referred to a palliative care team within 8 weeks of diagnosis. The benefits of early integrated palliative care are well recognized, however, there is a lack of consensus to guide operational aspects of a palliative care service within a comprehensive cancer center. In this study, we explore current palliative care referral patterns at an academic cancer center and provide recommendations for operationalizing palliative care services as a program within comprehensive cancer centers in order to adequately meet the needs of patients with advanced cancer.
Methods
A retrospective chart review of patients with newly diagnosed metastatic cancer or advanced hematologic malignancy seen by the palliative care team at a comprehensive cancer center from January 1, 2021, to October 31, 2021 was conducted. IRB approval was obtained prior to the initiation of the chart review.
Results
A total of 243 patients with newly diagnosed metastatic cancer, or advanced hematologic malignancy were included in this review. Patients with gastrointestinal (26%), gynecologic (19%), and thoracic (21%) malignancies constituted 66% of the total cohort. The most frequent reason for referral was pain (52%). Thirty-nine percent of patients were referred within 8 weeks of an advanced cancer diagnosis.
Conclusion
ASCO recommends that all patients with advanced cancer be referred to a palliative care specialist within 8 weeks of diagnosis. Of the newly referred patients with advanced cancer, only 39% were referred to the palliative care team within 8 weeks of their diagnosis. This large gap suggests the need for a consensus with regard to operationalizing the palliative care team.
Dendrochoronological Study on the Common Trees in Wadi Allaqi Biosphere Reserve, South-East Egypt
Authors: Salem Ashraf H. , Shedded Mohamed G. , Al-Sodany Yassin , Shaltout Kamal H.
Published: September 2022

Background
Twenty-five increment cores and tree discs were sampled for 4 trees species in Wadi Allaqi, an extremely arid region in South-East Egypt. All the studied species form distinct tree-ring boundaries. Of those, 19 samples for Acacia tortilis subsp. raddiana (Savi) Brenan, 2 for each of Balanites aegyptiaca (L.) Delile, Acacia ehrenbergiana Hayne, and Tamarix nilotica (Ehrenb.) Bunge. So, the main aim of the current study is to achieve a longer temporal perspective on growth and longevity, marginal parenchyma wood samples.
Results
The growth ring boundaries of the acacias are differentiated by thin parenchyma bands, which run around the entire stem disc. Samples of Acacia tortilis subsp. raddiana were located in the up-, down- and mid-stream parts of this Wadi; and classified, based on the mean distance between the bands of marginal parenchyma, into 6 size classes. Longevity based on the marginal parenchyma bands indicated that Acacia tortilis subsp. raddiana grew slowly and some of its studied individuals reflected 2 age scenarios in the downstream part, while the measurement interval an established date of around 1884 or 1886 is suggested. Both scenarios grew fast over a long period of time, and so the chiefly recent growth dated back to 1885. Approximate dates for the midstream part dated back to 1648, while the overall growth for the upstream part dated back to 1482. Samples of Balanites aegyptiaca may be established between 1608 and 1715, while those of Acacia ehrenbergiana may be established between 1945 and 1975. Tamarix nilotica swiftly established itself, and a new ecosystem replaced the severe arid habitat after the water level in Wadi Allaqi dropped in the 1980s.
Conclusion
Two scenarios of age are probably true for the downstream part, implying a date of establishment between 1884–1886. Balanites aegyptiaca was thought to have been established around 1715 or 1608, whereas Acacia ehrenbergiana was thought to have been established around 1975 or 1945. It was also discovered that the outdated scenario for Balanites aegyptiaca and Acacia ehrenbergiana is more in line with asymptotic value and current growth, indicating larger possibility for future expansion.
Molecular characterization of Anaplasma ovis Msp4 protein in strains isolated from ticks in Turkey: A multi-epitope synthetic vaccine antigen design against Anaplasma ovis using immunoinformatic tools
Authors: Köseoğlu Ahmet Efe , Can Hüseyin , Güvendi Mervenur , Alak Sedef Erkunt , Döşkaya Aysu Değirmenci , Karakavuk Muhammet , Döşkaya Mert , Ün Cemal
Published: September 2022

Background: Tick-borne pathogens increasingly threaten animal and human health as well as cause great economic loss in the livestock industry. Among these pathogens, Anaplasma oviscausing a decrease in meat and milk yield is frequently detected in sheep in many countries including Turkey. Unfortunately a vaccine has not been developed against Anaplasma ovis yet. Msp4 is a immunogenic surface protein of Anaplasma bacteria. This study aimed to reveal potential vaccine candidate epitopes in Msp4 protein using sequence data belonging to Msp4 gene of Anaplasma ovis isolates and then to design a multi-epitope protein to be used in vaccine formulations against Anaplasma ovis.
Results: For this purpose, Msp4 gene was sequenced from Anaplasma ovis isolates (n:6) detected in ticks collected from sheep in Turkey and the sequence data was compared with previous sequences from different countries in order to detect the variations of Msp4 gene/protein. Later, potential vaccine candidate and diagnostic epitopes were predicted using various immunoinformatics tools including physicochemical parameters, secondary structures, antigenicity, solubility, subcellular localization, transmembrane helix, signal peptide, allergenicity, BetaWrap motif, homology checking, postranslational modifications, B/T cell epitopes, toxicity, solvent exposure, and epitope conservancy. Among the discovered vaccine candidate epitopes, antigenic and conserved were selected, and then a multi-epitope protein was designed. The designed vaccine protein was tested for the assessment of TLR-2, IgG, and IFN-g responses by molecular docking and immune simulation analyses. Finally, among the discovered epitopes of Anaplasma ovis, EVASEGSGVM and YQFTPEISLV epitopes that have properties of high antigenicity, non-allergenicity, and non-toxicity were proposed for the development of peptide-based vaccines and serodiagnostic assays.
Conclusions: This study provides prominent epitopes and a candidate vaccine model to be used for the tick-borne pathogen Anaplasma ovis in further serodiagnostic and vaccine studies.
Foot and ankle characteristics associated with fear of falling and mobility in community-dwelling older people
Authors: Pol Fateme , Khajooei Zahra , Hosseini Sayed Mohsen , Taheri Alireza , Forghany Saeed , Menz Hylton
Published: September 2022

Background
Fear of falling is multifactorial in etiology and is associated with falls. It has been demonstrated that foot problems increase the risk of falls in older people. Therefore, the objective of this study was to investigate the associations of foot and ankle characteristics with fear of falling and mobility in community-dwelling older people.
Method
One hundred and eighty-seven community-dwelling older adults (106 females) aged 62–90 years (mean 70.5 ± 5.2) from Isfahan, Iran, were recruited. Foot and ankle characteristics (including foot posture, range of motion, muscle strength, deformity, tactile sensation, pain and dynamic function), fear of falling (Fall Efficacy Scale International) and mobility (Timed Up and Go Test) were measured. Two multivariate linear regression analyses identified variables independently associated with fear of falling and mobility.
Results
Linear regression analysis revealed that less ankle plantarflexor muscle strength, greater pressure-time integral, foot pain, and reduced tactile sensitivity of the ankle were significantly and independently associated with increased fear of falling. The total variance explained by the model was 59%. Less ankle plantarflexor muscle strength, greater pressure-time integral, and decreased centre of pressure velocity were significantly and independently associated with poorer mobility. The total variance explained by the model was 48%.
Conclusion
Several foot and ankle characteristics are associated with fear of falling and mobility in older people. Targeting these modifiable risk factors may play a role in reducing fear of falling and enhancing mobility performance in this population.
Bystanders’ willingness to perform basic life support and its relationship with facilitative and obstructive factors: A nationwide survey in Japan
Authors: Hasegawa Yoshiyuki , Hanaki Keiichi
Published: September 2022

Background: The administration of basic life-support (BLS) by bystanders is essential to improve the survival rates of patients with out-of-hospital cardiac arrest (OHCA). Although providing BLS to individuals who experience an OHCA greatly improves their chances of survival, the actual implementation rate is low. Therefore, we investigated the association between the bystanders’ willingness to perform BLS and facilitative/obstructive factors, with the objective of identifying educational methods that would improve the likelihood of bystanders performing BLS should they encounter a patient with OHCA.
Methods: The study participants included 1000 male and female Japanese residents aged 15–65 years with no experience in performing BLS, and 101 with experience, who consented to participate in the study. Both groups graded the strength of their willingness to perform BLS in future on a 4-point scale, as well as their level of agreement with factors facilitating or obstructing their willingness to perform BLS. These factors were designated on the basis of the helping behavior theory. We then analyzed the associations between them.
Results: The willingness to perform BLS decreased in accordance with the increase in the level of intervention required concerning the victim and was significantly associated with three facilitating factors: sufficient ability and experience to perform BLS, high personal norms, and a psychological closeness to the victim.
Conclusion: Our results suggested that workshops and other educational activities focused on these facilitative factors may be helpful in increasing the rate at which bystanders perform BLS.
Phytoplankton as Biomonitoring agent in two Perennial lakes of Coimbatore city, Tamil Nadu, India
Authors: Mohan Bala , Priyadarshinee Sunder Sheela
Published: September 2022

The present study evaluate the assessment of phytoplankton as pollution indicator using Palmer index were carried out at two perennial lakes of Coimbatore city. The bio-monitoring process by using phytoplankton provide cost-effective of the level of eutrophication. Bio-monitor activities focusing on major physico-chemical, biological compartments are needed to evaluate the condition of aquatic resources, as well as related environmental parameters. During the study period totally 36 species of phytoplankton recorded under 23 genera, which include 14 species of Cyanophyceae, 10 species of Chlorophyceae, 9 species of Bacillariophyceae and 3 species of Euglenophyceae. The study revealed that phytoplankton population density was dominated by following order Cyanophyceae ˃ Chlorophyceae ˃ Bacillariophyceae ˃ Euglenophyceae in two perennial lakes. In the study two perennial lakes indicates that the water is exposed to the organic pollution. The continuous monitoring of the lake is necessary.
Rotor Topology to Reach Beyond IE5 Ultra-Premium Efficiency in Line Start Permanent Magnet Synchronous Motor
Authors: P Sethupathi , Senthilnathan N. , Atshaya M.
Published: September 2022

Emerging efficiency standards indicates the demand of implementing highly efficient motors to reduce energy consumption and global warming, which is a worldwide concern. Owing to this, IE4 Efficiency line start permanent magnet synchronous motors (LSPMSM) contribution is increasing to a greater extent in the industrial motor market. This paper presents a new rotor configuration for a 2.2kW, 4 pole LSPMSM which is designed to meet efficiency beyond IE5 along with good power factor. The steady state and transient performance of the motor is optimized by using finite element analysis (FEA) software. From the results, it is evident that the proposed motor meets beyond IE5 efficiency level with 10% reduction in its length. Further reducing the length up to 30%, the motor sustains at IE4 efficiency level. To illustrate the optimum performance of the proposed design, the performance of the motor is benchmarked with identical radial type LSPMSM and commercially available IE2 induction motor. The FEA results of the proposed design are validated with the prototype development and testing. Consequently, this topology is both technically competent and cost effective, particularly for round the clock operation.
The inhibition of putrescine synthesis affects the in vitro shoot development of Cedrela fissilis Vell. (Meliaceae) by altering endogenous polyamine metabolism and the proteomic profile
Authors: Aragão Victor Paulo Mesquita , de Sousa Kariane Rodrigues , de Oliveira Tadeu Dos Reis , de Oliveira Leandro Francisco , Floh Eny Iochevet Segal , Silveira Vanildo , Santa-Catarina Claudete
Published: September 2022

Polyamines (PAs) and proteins have been demonstrated to be fundamental for in vitro shoot development of Cedrela fissilis. We evaluated the influence of 6-benzyladenine (BA) and putrescine (Put) on the growth of shoots, PA metabolism and proteomic profiles of C. fissilis.The longest shoots were obtained under 2.5 μM BA + 2.5 mM Put treatment. The inhibition of Put synthesis by D-arginine (D-arg) reduced the activities of the Put biosynthesis enzymes arginine decarboxylase (ADC) and ornithine decarboxylase (ODC) and the endogenous contents of free Put, resulting the reduced shoot growth. The ODC activity was higher than that ofADC, which is the main enzyme in the synthesis of Put in C. fissilis. Inhibition of Put synthesis affected the proteomic profile, reducing the accumulation of the ubiquitin receptor RAD23c, peroxidase 15, ADP-ribosylation factor 1, ADP-ribosylation factor-like protein 8a, profilin-4, profilin-2, glucan endo-1,3-beta-glucosidase, and expansin-like B1 and increasing the accumulation of V-type proton ATPase catalytic subunit A and methionine gamma-lyase, highlighting the relevance of these proteins in promoting the length of shoots. Moreover, the transport protein SEC13 homolog B and the basic isoform glucan endo-1,3-beta-glucosidase, unique proteins in shoots treated with BA+Put, were related to the promotion of shoot growth. Our results show that the modulation of endogenous PAs and proteomic profiles is necessary to regulate in vitro morphogenesis in C. fissilis. Moreover, the ODC enzyme is highly involved in the synthesis of Put during in vitro shoot development and is described for the first time in this species.
Expanding the Legionella pan-kinome
Authors: Krysińska Marianna , Baranowski Bartosz , Deszcz Bartłomiej , Pawłowski Krzysztof , Gradowski Marcin
Published: September 2022

The pathogenic Legionella bacteria are notorious for delivering numerous effector proteins into the host cell with the aim of disturbing and hijacking cellular processes for their benefit. Despite intensive studies, many effectors remain uncharacterized. Motivated by the richness of Legionella effector repertoires and their oftentimes atypical biochemistry, also by several known atypical Legionella effector kinases and pseudokinases, we undertook an in silico survey and exploration of the pan-kinome of the Legionella genus, i.e., the union of the kinomes of individual species. In this study, we discovered 13 novel (pseudo)kinase families (all are potential effectors) with the use of non-standard bioinformatic approaches. Together with 16 known families, we present a catalog of effector and non-effector protein kinase-like families within Legionella. We analyze and discuss the likely functional roles of the novel predicted kinases. Notably, some of the kinase families are also present in other bacterial taxa, including other pathogens, often phylogenetically very distant from Legionella. This work highlights Nature's ingeniousness in the pathogen–host arms race and offers a useful resource for the study of infection mechanisms.
Demographic and socio-economic factors influencing financial preparedness and the level of preparedness of salaried women in India
Authors: Radhakrishnan Sreeja , N Suresh V , Johnson Merin
Published: September 2022

People spend more than what they have and when it comes to saving money, most of the people save whatever is left over after consumption, without a proper budget for household, a proper goal, proper allocation of income, and proper understanding of investment avenues. Saving for retirement is harder for the present generation as the financial sector is overwhelmed with choices and specifically, women have little or no understanding about these investment avenues. As circumstances differ, women are experiencing dearth of financial wellbeing. The covid-19 crisis has increased the uncertainties and triggered the financial stress. This study brings insight into the financial preparedness level of women especially salaried and the influence of various demographic factors on financial preparedness. The questionnaire used for the study was developed through a series of steps and was further tested for reliability and validity. Statistical Package for Social Sciences (SPSS) 20.0 version was used for preliminary analysis of data and Analysis of Moment Structures Software-18 graphics (AMOS) were used for Confirmatory Factor Analysis and Structural Equational Modelling along with Microsoft excel 2016 for data entry of 600 respondents and analyzed using Mean, Standard deviation, Mean score analysis one-way Analysis of Variance, Post hoc test. As this research’s main contribution, findings on the level of financial preparedness of salaried women can be of help to those seeking to improve the lives of women. It will provide a foundation for policy formulation, training programmes, pension schemes development for empowering women.
SPOP Expression Is Associated with tumor-infiltrating lymphocytes in Pancreatic Cancer
Authors: Yang Xiaojuan , Xu Yongfeng , Zhu Qing
Published: September 2022

Background: Speckle Type POZ Protein (SPOP), despite its tumor type-dependent role in tumorigenesis, primarily as a tumor suppressor gene is associated with a variety of different cancers. However, its function in pancreatic cancer remains uncertain.
Methods: SPOP expression and the association between its expression and patient prognosis and immune function were evaluated using The Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx), The Tumor Immune Estimation Resource 2.0 (TIMER2.0) database, cBioportal, and various bioinformatic databases. Enrichment analysis of SPOP and the association between SPOP expression with clinical stage and grade were analyzed using the R software package. Then immunohistochemistry (IHC) was used to estimate the correlation between SPOP and tumor-infiltrating lymphocytes (TILs) in patients with pancreatic cancer.
Results: As part of our study, we assessed that SPOP was anomalously expressed in kinds of cancers, associated with clinical stage and outcomes. Meanwhile, SPOP also played a crucial role in the tumor microenvironment (TME). The expression level of SPOP was significantly correlated to tumor-infiltrating immune cells (TICs) in pancreatic cancer.
Conclusions: Our study uncovered the potential corrections in SPOP with TICs, suggesting that SPOP may act as a biomarker for immunotherapy in pancreatic cancer.
A systematic study on occurrence, risk estimation and health implications of heavy metals in potable groundwater of Garhwal Himalaya, India
Authors: Prasad Mukesh , Aswal R. S. , Joshi Abhishek , Kumar G. Anil , Ramola R. C.
Published: September 2022

Groundwater is consumed by major part of the population for drinking and other purposes. The occurrence of heavy metals (HMs) in drinking water has been a critical water quality concern for a long time and can compromise its aesthetic value to the larger extent. Chronic exposure of human beingsto these toxic and non-toxic HMs through water ingestion can result in significant health risks.To assess these associated health risks,the present study was planned, designed and carried out for analyses of nine HMs namely Al, Cr, Mn, Co, Ni, Cu, Zn, Cd, and Pb in the potable groundwater samples collected from different sources located across the Mandakini valley of Garhwal Himalaya, India using ICPMS. The observed values of analyzed HMs except Zn and Pb were found below the reference values prescribed by the WHO, USEPA, and BIS. The highvalue of Pb (11.252 µg l− 1 at one site) may be ascribed due to the corrosion of materials contained with lead or copper and household plumbing systemswhile Zn concentration exceeded its MPL (4000 µg l− 1) recommended by WHO for infants only at one station. These observed concentrations were further used to calculate HMs pollution indices to determine water quality based on their classification for assessment of health risks.The observed indicesshow that there are no health risks from HMs contamination via drinking water in the region. Moreover, the estimated hazard quotients for children and adults also revealed no potential health risks.The details of measurement protocols, employed methodology, and the results obtained are discussed below.
A penalized criterion for selecting the number of clusters for K-medians
Authors: Godichon-Baggioni Antoine , Surendran Sobihan
Published: September 2022

Clustering is a usual unsupervised machine learning technique for grouping the data points into groups based upon similar features. We focus here on unsupervised clustering for contaminated data, i.e in the case where K-medians algorithm should be preferred to K-means because of its robustness. More precisely, we concentrate on a common question in clustering: how to chose the number of clusters? The answer proposed here is to consider the choice of the optimal number of clusters as the minimization of a penalized criterion. In this paper, we obtain a suitable penalty shape for our criterion and derive an associated oracle-type inequality. Finally, the performance of this approach with different types of K-medians algorithms is compared on a simulation study with other popular techniques. All studied algorithms are available in the R package Kmedians on CRAN.
EEG Microstate Dynamics Associated with Dream-like Experiences during the Transition to Sleep
Authors: Diezig Sarah , Denzer Simone , Achermann Peter , Mast Fred W. , Koenig Thomas
Published: September 2022

Consciousness always requires some representational content; that is, one can only be conscious about something. However, the presence of conscious experience (awareness) alone does not determine whether its content is in line with the external and physical world. Dreams, apart from certain forms of hallucinations, typically consist of non-veridical percepts, which are not recognized as false, but rather considered real. This type of experiences have been described as a state of dissociation between phenomenal and reflective awareness. Interestingly, during the transition to sleep, reflective awareness seems to break down before phenomenal awareness as conscious experience does not immediately fade with reduced wakefulness but is rather characterized by the occurrence of uncontrolled thinking and perceptual images, together with a reduced ability to recognize the internal origin of the experience. Relative deactivation of the frontoparietal and preserved activity in parieto-occipital networks has been suggested to account for dream-like experiences during the transition to sleep. We tested this hypothesis by investigating subjective reports of conscious experience and large-scale brain networks using EEG microstates in 45 healthy young subjects during the transition to sleep. We observed an inverse relationship between cognitive effects and physiological activation; dream-like experiences were associated with an increased presence of a microstate with sources in the superior and middle frontal gyrus and precuneus. Additionally, the presence of a microstate associated with higher-order visual areas was decreased. The observed inverse relationship might therefore indicate a disengagement of cognitive control systems that is mediated by specific, inhibitory EEG microstates.