bluuebunny/crossref_metadata_2025 huggingface dataset:
Synthetic Study of Boulder Detection Using Multi-Configuration Combination of Cross-Hole ERT and Its Field Application in Xiamen Metro, China
Authors: Li Ningbo , Dong Zhao , Liu Zhengyu , Yan Bing , Wang Kai , Nie Lichao , Lin Chunjin , Shen Junfeng , Ma Zhao , Zhang Yongheng
Published: December 2021

With the fast development of urban rail transportation, shield construction has been widely used in tunnel construction in China. It is very important to detect the random distribution of boulders, which may cause geological problems. Cross-hole electrical resistivity tomography (ERT) has the advantage of precise detection, but it has not been systematically studied in solitary rock detection. Therefore, we set up several numerical models and proposed weighted inversion, to improve the capacity for detecting boulders. Subsequently, the method was applied to the Xiamen Metro Line 1, where it was highly likely to encounter boulders. All the test boreholes revealed the presence of boulders, and their location and size were consistent with the geophysics results. This study demonstrated the suitability of weighted inversion based on a multi-configuration combination of cross-hole ERT for the detection of boulders. This case study provides new engineering perspectives on how to detect boulders.
Job Insecurity and Work Meaning among Romanian Sports Coaches
Authors: Cosma Germina-Alina , Chiracu Alina , Stepan Amalia Raluca , Barbu Dumitru , Brabiescu-Călinescu Maria Luminița , Voinea Florin , Orțănescu Dorina , Țifrea Corina , Munteanu Robert-Valentin
Published: December 2021

The present study analyzed the relationships among material difficulties, job insecurity, work meaning and the mental health of sports coaches. Participants for this study were 200 coaches (120 men and 80 women, aged between 18 and 60 years, M = 41.04, SD = 8.71). The instruments used were (a) The Difficulties List, (b) Qualitative Job Insecurity, (c) Quantitative Job Insecurity, (d) The Work and Meaning Inventory, Depression, Anxiety, and Stress Scales. Statistical analysis was performed through SPSS 24. The results obtained by three multiple mediations showed that material difficulties were not directly associated with depression, anxiety, and stress. Although qualitative job insecurity mediates the relationship between material difficulties and mental health (β = 0.14, p < 0.01 for depression, β = 0.17, p < 0.01 for anxiety, and β = 0.14, p < 0.01 for stress) quantitative job insecurity and work meaning do not mediate this relationship but had significant direct effects. The conclusions of the study led to the need for material and psychological support for coaches to have a meaningful activity, so that they could achieve significant performance with their athletes.
Disease and Defect Detection System for Raspberries Based on Convolutional Neural Networks
Authors: Naranjo-Torres José , Mora Marco , Fredes Claudio , Valenzuela Andres
Published: December 2021

Raspberries are fruit of great importance for human beings. Their products are segmented by quality. However, estimating raspberry quality is a manual process carried out at the reception of the fruit processing plant, and is thus exposed to factors that could distort the measurement. The agriculture industry has increased the use of deep learning (DL) in computer vision systems. Non-destructive and computer vision equipment and methods are proposed to solve the problem of estimating the quality of raspberries in a tray. To solve the issue of estimating the quality of raspberries in a picking tray, prototype equipment is developed to determine the quality of raspberry trays using computer vision techniques and convolutional neural networks from images captured in the visible RGB spectrum. The Faster R–CNN object-detection algorithm is used, and different pretrained CNN networks are evaluated as a backbone to develop the software for the developed equipment. To avoid imbalance in the dataset, an individual object-detection model is trained and optimized for each detection class. Finally, both hardware and software are effectively integrated. A conceptual test is performed in a real industrial scenario, thus achieving an automatic evaluation of the quality of the raspberry tray, in this way eliminating the intervention of the human expert and eliminating errors involved in visual analysis. Excellent results were obtained in the conceptual test performed, reaching in some cases precision of 100%, reducing the evaluation time per raspberry tray image to 30 s on average, allowing the evaluation of a larger and representative sample of the raspberry batch arriving at the processing plant.
Optimising the Performance of Complex Sociotechnical Systems in High-Stress, High-Speed Environments: The Formula 1 Pit Stop Test Case
Authors: Slater David , Hill Rees , Kumar Maneesh , Ale Ben
Published: December 2021

In analysing the performance of complex sociotechnical systems, of particular interest is the inevitable and inherent variability that these systems exhibit, but can normally tolerate, in successfully operating in the real world. Knowing how that variability propagates and impacts the total function mix then allows an understanding of emergent behaviours. This interdependence, however, is not readily apparent from normal linear business process flow diagrams. An alternative approach to exploring the operability of complex systems, that addresses these limitations, is the functional resonance analysis method (FRAM). This is a way of visualising a system’s behaviour, by defining it as an array of functions, with all the interactions and interdependencies that are needed for it to work successfully. Until now this methodology has mainly been employed as a qualitative mind map. This paper describes a new development of the FRAM visualisation software that allows the quantification of the extent and effects of this functional variability. It then sets out to demonstrate its application in a practical, familiar test case. The example chosen is the complex sociotechnical system involved in a Formula 1 pit stop. This has shown the potential of the application and provided some interesting insights into the observed performances.
Electrochemical Characterization of Novel Polyantimonic-Acid-Based Proton Conductors for Low- and Intermediate-Temperature Fuel Cells
Authors: Kurapova Olga Yu. , Faia Pedro M. , Zaripov Artem A. , Pazheltsev Vasily V. , Glukharev Artem A. , Konakov Vladimir G.
Published: December 2021

The development of novel proton-conducting membrane materials for electrochemical power units, i.e., low temperature fuel cells (FCs), efficiently working up to 300 °C, is a critical problem related to the rapid shift to hydrogen energy. Polyantimonic acid (PAA) is characterized by high conductivity, sufficient thermal stability and can be regarded as a prospective proton-conducting material. However, the fabrication of bulk PAA-based membranes with high proton conductivity remains a challenging task. In the present work, for the first time, the authors report the investigation on proton conductivity of bulk PAA-based membranes in the temperature range 25–250 °C, both in dry air and in moisturized air. Using PAA powder and fluoroplastic as a binder, fully dense cylindrical membranes were formed by cold uniaxial pressing. The structures of the PAA-based membranes were investigated by SEM, EDX, XRD and Raman techniques. STA coupled with in situ thermo-XRD analysis revealed that the obtained membranes corresponded with Sb2O5·3H2O with pyrochlore structure, and that no phase transitions took place up to 330 °C. PAA-based membranes possess a high-grain component of conductivity, 5 × 10−2 S/cm. Grain boundary conductivities of 90PAA and 80PAA membranes increase with relative humidity content and their values change non-linearly in the range 25–250 °C.
Measuring Polarization in Online Debates
Authors: Alsinet Teresa , Argelich Josep , Béjar Ramón , Martínez Santi
Published: December 2021

Social networks can be a very successful tool to engage users to discuss relevant topics for society. However, there are also some dangers that are associated with them, such as the emergence of polarization in online discussions. Recently, there has been a growing interest to try to understand this phenomenon, as some consider that this can be harmful concerning the building of a healthy society in which citizens get used to polite discussions and even listening to opinions that may be different from theirs. In this work, we face the problem of defining a precise measure that can quantify in a meaningful way the level of polarization present in an online discussion. We focus on the Reddit social network, given that its primary focus is to foster discussions, in contrast to other social networks that have some other uses. Our measure is based on two different characteristics of an online discussion: the existence of a balanced bipartition of the users of the discussion, where one partition contains mainly users in agreement (regarding the topic of the discussion) and the other users in disagreement, and the degree of negativity of the sentiment of the interactions between these two groups of users. We discuss how different characteristics of the discussions affect the value of our polarization measure, and we finally perform an empirical evaluation over different sets of Reddit discussions about diverse classes of topics. Our results seem to indicate that our measure can capture differences in the polarization level of different discussions, which can be further understood when analyzing the values of the different factors used to define the measure.
Machine Learning Based Color Classification by Means of Visually Evoked Potentials
Authors: Böck Carl , Meier Lea , Kalb Stephan , Vosko Milan R. , Tschoellitsch Thomas , Huemer Mario , Meier Jens
Published: December 2021

Visually evoked potentials (VEPs) are widely used for diagnoses of different neurological diseases. Interestingly, there is limited research about the impact of the stimulus color onto the evoked response. Therefore, in our study we investigated the possibility of automatically classifying the stimulus color. The visual stimuli were selected to be red/black and green/black checkerboard patterns with equal light density. Both of these stimuli were presented in a random manner to nine subjects, while the electroencephalogram was recorded at the occipital lobe. After pre-processing and aligning the evoked potentials, an artificial neural network with one hidden layer was used to investigate the general possibility to automatically classify the stimulus color in three different settings. First, color classification with individually trained models, color classification with a common model, and color classification for each individual volunteer with a model trained on the data of the remaining subjects. With an average accuracy (ACC) of 0.83, the best results were achieved for the individually trained model. Also, the second (mean ACC = 0.76) and third experiments (mean ACC = 0.71) indicated a reasonable predictive accuracy across all subjects. Consequently, machine learning tools are able to appropriately classify stimuli colors based on VEPs. Although further studies are needed to improve the classification performance of our approach, this opens new fields of applications for VEPs.
Real-Time Remote Sensing of the Lobesia botrana Moth Using a Wireless Acoustic Detection Sensor
Authors: Hermosilla Gabriel , Pizarro Francisco , Fingerhuth Sebastián , Lazcano Francisco , Santibanez Francisco , Baker Nelson , Castro David , Yáñez Carolina
Published: December 2021

This article presents a wireless sensor for pest detection, specifically the Lobesia botrana moth or vineyard moth. The wireless sensor consists of an acoustic-based detection of the sound generated by a flying Lobesia botrana moth. Once a Lobesia botrana moth is detected, the information about the time, geographical location of the sensor and the number of detection events is sent to a server that gathers the detection statistics in real-time. To detect the Lobesia botrana, its acoustic signal was previously characterized in a controlled environment, obtaining its power spectral density for the acoustic filter design. The sensor is tested in a controlled laboratory environment where the detection of the flying moths is successfully achieved in the presence of all types of environmental noises. Finally, the sensor is installed on a vineyard in a region where the moth has already been detected. The device is able to detect flying Lobesia botrana moths during its flying period, giving results that agree with traditional field traps.
Enhancing Recommender System with Collaborative Filtering and User Experiences Filtering
Authors: Aciar Silvana Vanesa , Fabregat Ramon , Jové Teodor , Aciar Gabriela
Published: December 2021

Recommender systems have become an essential part in many applications and websites to address the information overload problem. For example, people read opinions about recommended products before buying them. This action is time-consuming due to the number of opinions available. It is necessary to provide recommender systems with methods that add information about the experiences of other users, along with the presentation of the recommended products. These methods should help users by filtering reviews and presenting the necessary answers to their questions about recommended products. The contribution of this work is the description of a recommender system that recommends products using a collaborative filtering method, and which adds only relevant feedback from other users about recommended products. A prototype of a hotel recommender system was implemented and validated with real users.
Model-Validation and Implementation of a Path-Following Algorithm in an Autonomous Underwater Vehicle
Authors: Villa Jose , Vallicrosa Guillem , Aaltonen Jussi , Ridao Pere , Koskinen Kari T.
Published: December 2021

This article studies the design, modeling, and implementation of a path-following algorithm as a guidance, navigation, and control (GNC) architecture for an autonomous underwater vehicle (AUV). First, a mathematical model is developed based on nonlinear equations of motion and parameter estimation techniques, including the model validation based on field test data. Then, the guidance system incorporates a line-of-sight (LOS) algorithm with a combination of position PID controllers. The GNC architecture includes a modular and multi-layer approach with an LOS-based, path-following algorithm in the AUV platform. Furthermore, the navigation used in the path-following algorithm is developed based on a predefined coverage area. Finally, this study addresses simulation and field test control scenarios to verify the developed GNC architecture.
Numerical Study of the Unsteady Aerodynamic Performance of Two Maglev Trains Passing Each Other in Open Air Using Different Turbulence Models
Authors: Li Xianli , Krajnovic Siniša , Zhou Dan
Published: December 2021

The strong change in the flow fields around two maglev trains (MTs) passing each other in open air may affect their manoeuvrability and passengers’ comfort. In this study, we evaluated the aerodynamic performance of two MTs passing each other via shear stress transport (SST) k–ω model and improved delayed detached eddy simulations based on the Spalart–Allmaras model (SA−IDDES) and the SST k–ω model (SST−IDDES). The accuracy of the numerical simulation method was verified using experimental data acquired from a moving model test. The results showed that the difference in the amplitude of the transient pressure obtained with the different turbulence models was less than 5%. The wake vortex structures on the intersection side were found to interact, and their intensity consequently decreased. The SST−IDDES model produced smaller-scale vortices than the SA−IDDES model, particularly in the near-wake region. There were large differences in the drag and lift forces obtained using the different turbulence models. Among them, the lift force of the tail car was more sensitive to the turbulence model, and its maximum value obtained with the SST−IDDES model was 11% larger than that obtained with the SA−IDDES model.
A Comprehensive Review of Microencapsulated Phase Change Materials Synthesis for Low-Temperature Energy Storage Applications
Authors: Hamad Ghada Ben , Younsi Zohir , Naji Hassane , Salaün Fabien
Published: December 2021

Thermal energy storage (TES) using phase change materials (PCMs) is an innovative approach to meet the growth of energy demand. Microencapsulation techniques lead to overcoming some drawbacks of PCMs and enhancing their performances. This paper presents a comprehensive review of studies dealing with PCMs properties and their encapsulation techniques. Thus, it is essential to critically examine the existing techniques and their compatibility with different types of PCMs, coating materials, and the area of application. The main objective of this review is to describe each microencapsulation process and to determine different factors that influence the performance of resulting microcapsules. Microencapsulation efficiency, as well as the limitation of each technique, are investigated, and optimum operating conditions of each process are highlighted. Furthermore, up-to-date studies of multifunctional PCMs microcapsules development with enhanced performances and new application directions are also presented. This review aims to be a useful guide for future researches dealing with low thermal energy storage applications of PCMs microcapsules.
Special Issue “Interaction of Ionizing Photons with Atomic and Molecular Ions”
Authors: Kennedy Eugene T. , Costello John T. , Mosnier Jean-Paul
Published: December 2021

The interaction of ionizing photons with atoms or ions is a fundamental process in nature, with laboratory, atmospheric and astrophysical implications [...]
Verification of a Mobile Psychoacoustic Test System
Authors: Soares Jordana C. , Veeranna Sangamanatha A. , Parsa Vijay , Allan Chris , Ly Winnie , Duong Minh , Folkeard Paula , Moodie Sheila , Allen Prudence
Published: December 2021

Many hearing difficulties can be explained as a loss of audibility, a problem easily detected and treated using standard audiological procedures. Yet, hearing can be much poorer (or more impaired) than audibility predicts because of deficits in the suprathreshold mechanisms that encode the rapidly changing, spectral, temporal, and binaural aspects of the sound. The ability to evaluate these mechanisms requires well-defined stimuli and strict adherence to rigorous psychometric principles. This project reports on the comparison between a laboratory-based and a mobile system’s results for psychoacoustic assessment in adult listeners with normal hearing. A description of both systems employed is provided. Psychoacoustic tests include frequency discrimination, amplitude modulation detection, binaural encoding, and temporal gap detection. Results reported by the mobile system were not significantly different from those collected with the laboratory-based system for most of the tests and were consistent with those reported in the literature. The mobile system has the potential to be a feasible option for the assessment of suprathreshold auditory encoding abilities.
Intracorporeal Cortical Telemetry as a Step to Automatic Closed-Loop EEG-Based CI Fitting: A Proof of Concept
Authors: Beynon Andy J. , Luijten Bart M. , Mylanus Emmanuel A. M.
Published: December 2021

Electrically evoked auditory potentials have been used to predict auditory thresholds in patients with a cochlear implant (CI). However, with exception of electrically evoked compound action potentials (eCAP), conventional extracorporeal EEG recording devices are still needed. Until now, built-in (intracorporeal) back-telemetry options are limited to eCAPs. Intracorporeal recording of auditory responses beyond the cochlea is still lacking. This study describes the feasibility of obtaining longer latency cortical responses by concatenating interleaved short recording time windows used for eCAP recordings. Extracochlear reference electrodes were dedicated to record cortical responses, while intracochlear electrodes were used for stimulation, enabling intracorporeal telemetry (i.e., without an EEG device) to assess higher cortical processing in CI recipients. Simultaneous extra- and intra-corporeal recordings showed that it is feasible to obtain intracorporeal slow vertex potentials with a CI similar to those obtained by conventional extracorporeal EEG recordings. Our data demonstrate a proof of concept of closed-loop intracorporeal auditory cortical response telemetry (ICT) with a cochlear implant device. This research breaks new ground for next generation CI devices to assess higher cortical neural processing based on acute or continuous EEG telemetry to enable individualized automatic and/or adaptive CI fitting with only a CI.
Lithium-Ion Battery Thermal Management Systems: A Survey and New CFD Results
Authors: Falcone Morena , Palka Bayard De Volo Eleonora , Hellany Ali , Rossi Claudio , Pulvirenti Beatrice
Published: December 2021

The environment has gained significant importance in recent years, and companies involved in several technology fields are moving in the direction of eco-friendly solutions. One of the most discussed topics in the automotive field is lithium-ion battery packs for electric vehicles and their battery thermal management systems (BTMSs). This work aims to show the most used lithium-ion battery pack cooling methods and technologies with best working temperature ranges together with the best performances. Different cooling methods are presented and discussed, with a focus on the comparison between air-cooling systems and liquid-cooling systems. In this context, a BTMS for cylindrical cells is presented, where the cells are arranged in staggered lines embedded in a solid structure and cooled through forced convection within channels. The thermal behavior of this BTMS is simulated by employing a computational fluid dynamics (CFD) approach. The effect of the geometry of the BTMS on the cell temperature distribution is obtained. It is shown that the use of materials with additives for the solid structure enhances the performance of the system, giving lower temperatures to the cells. The system is tested with air-cooling and water-cooling, showing that the best performances are obtained with water-cooling in terms of cell packing density and lowest cell temperatures.
Alcoholic Fermentation Monitoring and pH Prediction in Red and White Wine by Combining Spontaneous Raman Spectroscopy and Machine Learning Algorithms
Authors: Fuller Harrison , Beaver Chris , Harbertson James
Published: December 2021

In the following study, total sugar concentrations before and during alcoholic fermentation, as well as ethanol concentrations and pH levels after fermentation, of red and white wine grapes were successfully predicted using Raman spectroscopy. Fluorescing compounds such as anthocyanins and pigmented phenolics found in red wine present one of the primary limitations of enological analysis using Raman spectroscopy. Unlike the spontaneous Raman effect, fluorescence is a highly efficient process and consequently emits a much stronger signal than spontaneous Raman scattering. For this reason, many enological applications of Raman spectroscopy are impractical as the more subtle Raman spectrum of any red wine sample is in large part masked by fluorescing compounds present in the wine. This work employs a simple extraction method to mitigate fluorescence in finished red wines. Ethanol and total sugars (fructose plus glucose) of wines made from red (Cabernet Sauvignon) and white (Chardonnay, Sauvignon Blanc, and Gruner Veltliner) varieties were modeled using support vector regression (SVR), partial least squares regression (PLSR) and Ridge regression (RR). The results, which compared the predicted to measured total sugar concentrations before and during fermentation, were excellent (R2SVR = 0.96, R2PLSR = 0.95, R2RR = 0.95, RMSESVR = 1.59, RMSEPLSR = 1.57, RMSERR = 1.57), as were the ethanol and pH predictions for finished wines after phenolic stripping with polyvinylpolypyrrolidone (R2SVR = 0.98, R2PLSR = 0.99, R2RR = 0.99, RMSESVR = 0.23, RMSEPLSR = 0.21, RMSERR = 0.23). The results suggest that Raman spectroscopy is a viable tool for rapid and trustworthy fermentation monitoring.
Pheno-SELEX: Engineering Anti-Metastatic Aptamers through Targeting the Invasive Phenotype Using Systemic Evolution of Ligands by Exponential Enrichment
Authors: Shelley Greg , Dai Jinlu , Keller Jill M. , Keller Evan T.
Published: December 2021

Multiple methods (e.g., small molecules and antibodies) have been engineered to target specific proteins and signaling pathways in cancer. However, many mediators of the cancer phenotype are unknown and the ability to target these phenotypes would help mitigate cancer. Aptamers are small DNA or RNA molecules that are designed for therapeutic use. The design of aptamers to target cancers can be challenging. Accordingly, to engineer functionally anti-metastatic aptamers we used a modification of systemic evolution of ligands by exponential enrichment (SELEX) we call Pheno-SELEX to target a known phenotype of cancer metastasis, i.e., invasion. A highly invasive prostate cancer (PCa) cell line was established and used to identify aptamers that bound to it with high affinity as opposed to a less invasive variant to the cell line. The anti-invasive aptamer (AIA1) was found to inhibit in vitro invasion of the original highly invasive PCa cell line, as well as an additional PCa cell line and an osteosarcoma cell line. AIA1 also inhibited in vivo development of metastasis in both a PCa and osteosarcoma model of metastasis. These results indicate that Pheno-SELEX can be successfully used to identify aptamers without knowledge of underlying molecular targets. This study establishes a new paradigm for the identification of functional aptamers.
Survey of Keratinophilic Fungi from Feathers of Birds in Tuscany
Authors: Nardoni Simona , Mancianti Francesca
Published: December 2021

Although keratinophilic fungi on avian feathers have been widely described, data from European literature are quite lacking regarding Mediterranean countries. The aim of the present study was therefore to evaluate the occurrence of fungal species on feathers of different avian species in Italy. A total of 378 feather samples from both aquatic (n = 254) and terrestrial birds (n = 124), for a total of 30 bird species, were cultured for keratinophilic fungi. Fungal isolates were recognized by their macro- and micro-scopical morphology, and results were corroborated by PCR and sequencing. Keratinophilic fungi belonging to 11 different species (Scopulariopsis brevicaulis, Chrysosporium keratinophilum, Trichophyton terrestre, Microsporum gypseum, Sepedonium sp., Chrysosporium pannorum, Myriodontium sp., Chrysosporium tropicum, Chrysosporium pruinosum, Chrysosporium luteum and Aphanoascus fulvescens) were isolated from 71 animals (18.8%). The frequency of isolation of keratinophilic species from terrestrial birds was significantly higher in waterfowl. Migratory birds in Italy have been proven to carry pathogenic fungi such as dermatophytes, (A. platyrhyncos, A. crecca, E. rubecula), besides saprophytic species.
Potential of Asparagopsis armata as a Biopesticide for Weed Control under an Invasive Seaweed Circular-Economy Framework
Authors: Duarte Bernardo , Carreiras João , Feijão Eduardo , de Carvalho Ricardo Cruz , Matos Ana Rita , Fonseca Vanessa F. , Novais Sara C. , Lemos Marco F. L.
Published: December 2021

Marine macroalgae have been increasingly targeted as a source of bioactive compounds to be used in several areas, such as biopesticides. When harvesting invasive species, such as Asparagopsis armata, for this purpose, there is a two-folded opportunity: acquiring these biomolecules from a low-cost resource and controlling its spreading and impacts. The secondary metabolites in this seaweed’s exudate have been shown to significantly impact the physiology of species in the ecosystems where it invades, indicating a possible biocidal potential. Considering this in the present work, an A. armata exudate cocktail was applied in the model weed Thellungiella halophila to evaluate its physiological impact and mode of action, addressing its potential use as a natural biocide. A. armata greatly affected the test plants’ physiology, namely, their photochemical energy transduction pathway (impairing light-harvesting and chemical energy production throughout the chloroplast electron transport chain), carotenoid metabolism and oxidative stress. These mechanisms of action are similar to the ones triggered when using the common chemical pesticides, highlighting the potential of the A. armata exudate cocktail as an eco-friendly biopesticide.
Public Awareness and Perceptions of Invasive Alien Species in Small Towns
Authors: Jubase Nolwethu , Shackleton Ross T. , Measey John
Published: December 2021

Invasive alien species (IAS) are a growing threat globally and cause a variety of ecological, economic, and social impacts. People can introduce IAS and facilitate their spread, and can also implement, support, or oppose their management. Understanding local knowledge, awareness, and perceptions are therefore crucial if management and policy are to be effective. We administered questionnaires to members of the public in eight small towns along the Berg River Catchment in the biodiverse fynbos biome of South Africa. We aimed to assess: (1) awareness of IAS by the general public, (2) local perceptions of the impacts associated with IAS, (3) whether awareness of IAS is correlated with demographic covariates and IAS density, and (4) people’s willingness to detect, report, and support IAS management. Overall, 262 respondents participated in the survey. Most respondents (65%) did not know what IAS are, and 10% were unsure. Many respondents also perceived IAS as beneficial. Using a logistic regression, we found that IAS density, educational level, and gender influenced people’s knowledge and perceptions about IAS in the region. There were a small number (4%) of respondents currently detecting and reporting IAS, but many respondents were interested to learn more. We concluded that people living in small towns in the Western Cape of South Africa remain largely unaware of IAS and their impacts. It is crucial to increase awareness-raising initiatives, and build support and engagement in management of IAS in small towns.
Drug Repurposing for Glioblastoma and Current Advances in Drug Delivery—A Comprehensive Review of the Literature
Authors: Alomari Safwan , Zhang Irma , Hernandez Adrian , Kraft Caitlin Y. , Raj Divyaansh , Kedda Jayanidhi , Tyler Betty
Published: December 2021

Glioblastoma (GBM) is the most common primary malignant brain tumor in adults with an extremely poor prognosis. There is a dire need to develop effective therapeutics to overcome the intrinsic and acquired resistance of GBM to current therapies. The process of developing novel anti-neoplastic drugs from bench to bedside can incur significant time and cost implications. Drug repurposing may help overcome that obstacle. A wide range of drugs that are already approved for clinical use for the treatment of other diseases have been found to target GBM-associated signaling pathways and are being repurposed for the treatment of GBM. While many of these drugs are undergoing pre-clinical testing, others are in the clinical trial phase. Since GBM stem cells (GSCs) have been found to be a main source of tumor recurrence after surgery, recent studies have also investigated whether repurposed drugs that target these pathways can be used to counteract tumor recurrence. While several repurposed drugs have shown significant efficacy against GBM cell lines, the blood–brain barrier (BBB) can limit the ability of many of these drugs to reach intratumoral therapeutic concentrations. Localized intracranial delivery may help to achieve therapeutic drug concentration at the site of tumor resection while simultaneously minimizing toxicity and side effects. These strategies can be considered while repurposing drugs for GBM.
The MAP Kinase Phosphatase MKP-1 Modulates Neurogenesis via Effects on BNIP3 and Autophagy
Authors: Li Yinghui , Halterman Marc W.
Published: December 2021

Inherited and acquired defects in neurogenesis contribute to neurodevelopmental disorders, dysfunctional neural plasticity, and may underlie pathology in a range of neurodegenerative conditions. Mitogen-activated protein kinases (MAPKs) regulate the proliferation, survival, and differentiation of neural stem cells. While the balance between MAPKs and the family of MAPK dual-specificity phosphatases (DUSPs) regulates axon branching and synaptic plasticity, the specific role that DUSPs play in neurogenesis remains unexplored. In the current study, we asked whether the canonical DUSP, MAP Kinase Phosphatase-1 (MKP-1), influences neural stem cell differentiation and the extent to which DUSP-dependent autophagy is operational in this context. Under basal conditions, Mkp-1 knockout mice generated fewer doublecortin (DCX) positive neurons within the dentate gyrus (DG) characterized by the accumulation of LC3 puncta. Analyses of wild-type neural stem cell (NSC) differentiation in vitro revealed increased Mkp-1 mRNA expression during the initial 24-h period. Notably, Mkp-1 KO NSC differentiation produced fewer Tuj1-positive neurons and was associated with increased expression of the BCL2/adenovirus E1B 19-kD protein-interacting protein 3 (BNIP3) and levels of autophagy. Conversely, Bnip3 knockdown in differentiated Mkp-1 KO NSCs reduced levels of autophagy and increased neuronal yields. These results indicate that MKP-1 exerts a pro-neurogenic bias during a critical window in NSC differentiation by regulating BNIP3 and basal autophagy levels.
Effect of Sand-Frying-Triggered Puffing on the Multi-Scale Structure and Physicochemical Properties of Cassava Starch in Dry Gel
Authors: He Yonglin , Ye Fayin , Li Sheng , Wang Damao , Chen Jia , Zhao Guohua
Published: December 2021

This study revealed the underlying mechanisms involved in the puffing process of dried cassava starch gel by exploring the development of the puffed structure of gel upon sand-frying, chiefly focused on the changes in the multi-scale structure and the physicochemical properties of starch. The results suggested that the sand-frying-induced puffing proceeded very fast, completed in about twenty seconds, which could be described as a two-phase pattern including the warming up (0~6 s) and puffing (7~18 s) stages. In the first stage, no significant changes occurred to the structure or appearance of the starch gel. In the second stage, the cells in the gel network structure were expanded until burst, which brought about a decrease in moisture content, bulk density, and hardness, as well as the increase in porosity and crispness when the surface temperature of gel reached glass transition temperature of 125.28 °C. Upon sand-frying puffing, the crystalline melting and molecular degradation of starch happened simultaneously, of which the latter mainly occurred in the first stage. Along with the increase of puffing time, the thermal stability, peak viscosity, and final viscosity of starch gradually decreased, while the water solubility index increased. Knowing the underlying mechanisms of this process might help manufacturers produce a better quality of starch-based puffed products.
Essential Fatty Acids and Their Metabolites in the Pathobiology of Inflammation and Its Resolution
Authors: Das Undurti N
Published: December 2021

Arachidonic acid (AA) metabolism is critical in the initiation and resolution of inflammation. Prostaglandin E2 (PGE2) and leukotriene B4/D4/E4 (LTB4/LD4/LTE4), derived from AA, are involved in the initiation of inflammation and regulation of immune response, hematopoiesis, and M1 (pro-inflammatory) macrophage facilitation. Paradoxically, PGE2 suppresses interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) production and triggers the production of lipoxin A4 (LXA4) from AA to initiate inflammation resolution process and augment regeneration of tissues. LXA4 suppresses PGE2 and LTs’ synthesis and action and facilitates M2 macrophage generation to resolve inflammation. AA inactivates enveloped viruses including SARS-CoV-2. Macrophages, NK cells, T cells, and other immunocytes release AA and other bioactive lipids to produce their anti-microbial actions. AA, PGE2, and LXA4 have cytoprotective actions, regulate nitric oxide generation, and are critical to maintain cell shape and control cell motility and phagocytosis, and inflammation, immunity, and anti-microbial actions. Hence, it is proposed that AA plays a crucial role in the pathobiology of ischemia/reperfusion injury, sepsis, COVID-19, and other critical illnesses, implying that its (AA) administration may be of significant benefit in the prevention and amelioration of these diseases.
Deodorant Activity of Black Cumin Seed Essential Oil against Garlic Organosulfur Compound
Authors: Yoshioka Yuri , Matsumura Shinichi , Morimoto Masanori
Published: December 2021

The deodorant activity of black cumin (Nigella sativa L.) seed, a spice used to flavor curry and vegetable foods in Southwest Asia, against garlic (Allium sativum L.) organosulfur compounds related to human malodor was evaluated. Black cumin seed essential oil showed remarkable deodorant activity against garlic essential oil. The mode of action of this deodorant activity was presumed to be that black cumin seed essential oil covalently reacted with the organosulfur compounds in garlic. Therefore, thymoquinone, which is a major constituent in black cumin seed essential oil, and allyl mercaptan, which is one of the organosulfur compounds produced by cutting garlic, were reacted in vitro, and the products were purified and elucidated using spectroscopic data. As a result, these substances were identified as different allyl mercaptan adducts to dihydrothymoquinone. This chemical reaction was presumed to play a key role in the deodorant activity of black cumin seed essential oil.
Cell-Projection Pumping of Fibroblast Contents into Osteosarcoma SAOS-2 Cells Correlates with Increased SAOS-2 Proliferation and Migration, as well as Altered Morphology
Authors: Mahadevan Swarna , Cornwell James A , Chami Belal , Kelly Elizabeth , Zoellner Hans
Published: December 2021

We earlier reported that cell-projection pumping transfers fibroblast contents to cancer cells and this alters the cancer cell phenotype. Here, we report on single-cell tracking of time lapse recordings from co-cultured fluorescent fibroblasts and SAOS-2 osteosarcoma cells, tracking 5201 cells across 7 experiments. The fluorescent lipophilic marker DiD was used to label fibroblast organelles and to trace the transfer of fibroblast cytoplasm into SAOS-2 cells. We related SAOS-2 phenotypic change to levels of fluorescence transfer from fibroblasts to SAOS-2 cells, as well as what we term ‘compensated fluorescence’, that numerically projects mother cell fluorescence post-mitosis into daughter cells. The comparison of absolute with compensated fluorescence allowed us to deduct if the phenotypic effects in mother SAOS-2 cells were inherited by their daughters. SAOS-2 receipt of fibroblast fluorescence correlated by Kendall’s tau with cell-profile area and without evidence of persistence in daughter cells (median tau = 0.51, p < 0.016); negatively and weakly with cell circularity and with evidence of persistence (median tau = −0.19, p < 0.05); and very weakly with cell migration velocity and without evidence of persistence (median tau = 0.01, p < 0.016). In addition, mitotic SAOS-2 cells had higher rates of prior fluorescence uptake (median = 64.9 units/day) than non-dividing cells (median = 35.6 units/day, p < 0.016) and there was no evidence of persistence post-mitosis. We conclude that there was an appreciable impact of cell-projection pumping on cancer cell phenotype relevant to cancer histopathological diagnosis, clinical spread and growth, with most effects being ‘reset’ by cancer cell mitosis.
Physiological and Pathophysiological Roles of Mitochondrial Na+-Ca2+ Exchanger, NCLX, in Hearts
Authors: Takeuchi Ayako , Matsuoka Satoshi
Published: December 2021

It has been over 10 years since SLC24A6/SLC8B1, coding the Na+/Ca2+/Li+ exchanger (NCLX), was identified as the gene responsible for mitochondrial Na+-Ca2+ exchange, a major Ca2+ efflux system in cardiac mitochondria. This molecular identification enabled us to determine structure–function relationships, as well as physiological/pathophysiological contributions, and our understandings have dramatically increased. In this review, we provide an overview of the recent achievements in relation to NCLX, focusing especially on its heart-specific characteristics, biophysical properties, and spatial distribution in cardiomyocytes, as well as in cardiac mitochondria. In addition, we discuss the roles of NCLX in cardiac functions under physiological and pathophysiological conditions—the generation of rhythmicity, the energy metabolism, the production of reactive oxygen species, and the opening of mitochondrial permeability transition pores.
Therapy of Pseudoxanthoma Elasticum: Current Knowledge and Future Perspectives
Authors: Stumpf Max Jonathan , Schahab Nadjib , Nickenig Georg , Skowasch Dirk , Schaefer Christian Alexander
Published: December 2021

Pseudoxanthoma elasticum (PXE) is a rare, genetic, metabolic disease with an estimated prevalence of between 1 per 25,000 and 56,000. Its main hallmarks are characteristic skin lesions, development of choroidal neovascularization, and early-onset arterial calcification accompanied by a severe reduction in quality-of-life. Underlying the pathology are recessively transmitted pathogenic variants of the ABCC6 gene, which results in a deficiency of ABCC6 protein. This results in reduced levels of peripheral pyrophosphate, a strong inhibitor of peripheral calcification, but also dysregulation of blood lipids. Although various treatment options have emerged during the last 20 years, many are either already outdated or not yet ready to be applied generally. Clinical physicians often are left stranded while patients suffer from the consequences of outdated therapies, or feel unrecognized by their attending doctors who may feel uncertain about using new therapeutic approaches or not even know about them. In this review, we summarize the broad spectrum of treatment options for PXE, focusing on currently available clinical options, the latest research and development, and future perspectives.
Regional Diversities in Fibrogenesis Weighed as a Key Determinant for Atrial Arrhythmogenesis
Authors: Chung Cheng-Chih , Chin Chye-Gen , Lin Yung-Kuo , Chen Yao-Chang , Cheng Wan-Li , Yeh Yung-Hsin , Kao Yu-Hsun , Chen Yi-Jen
Published: December 2021

Atrial fibrosis plays a key role in atrial myopathy, resulting in the genesis of atrial fibrillation (AF). The abnormal distribution of fibrotic tissue, electrical coupling, paracrine interactions, and biomechanical–electrical interactions have all been suggested as causes of fibrosis-related arrhythmogenesis. Moreover, the regional difference in fibrogenesis, specifically the left atrium (LA) exhibiting a higher arrhythmogenesis and level of fibrosis than the right atrium (RA) in AF, is a key contributor to atrial arrhythmogenesis. LA fibroblasts have greater profibrotic cellular activities than RA fibroblasts, but knowledge about the regional diversity of atrial regional fibrogenesis remains limited. This article provides a comprehensive review of research findings on the association between fibrogenesis and arrhythmogenesis from laboratory to clinical evidence and updates the current understanding of the potential mechanism underlying the difference in fibrogenesis between the LA and RA.
Angiotensin System Modulations in Spontaneously Hypertensive Rats and Consequences on Erythrocyte Properties; Action of MLN-4760 and Zofenopril
Authors: Jasenovec Tomas , Radosinska Dominika , Kollarova Marta , Balis Peter , Dayar Ezgi , Bernatova Iveta , Zorad Stefan , Vrbjar Norbert , Cacanyova Sona , Radosinska Jana
Published: December 2021

Various pathologies (COVID-19 including) are associated with abnormalities in erythrocyte properties. Hypertension represents an unfavorable condition for erythrocyte quality and is the most prevalent risk factor in COVID-19 patients. ACE2 downregulation that is typical of these patients can further deteriorate cardiovascular health; however, its consequences on erythrocyte properties are not known yet. The aim was to investigate the effect of ACE2 inhibition and the potential beneficial effect of zofenopril on erythrocytes in spontaneously hypertensive rats. ACE2 inhibition induced by MLN-4760 (1 mg/kg/day for 2 weeks) led to deterioration of erythrocyte morphology and osmotic resistance, but plasma markers of oxidative stress, erythrocyte deformability, nitric oxide production and Na,K-ATPase activity were not significantly affected. Zofenopril administration (10 mg/kg/day, initiated after 4-day-lasting ACE2 inhibition) resulted in unexpected increase in angiotensin II plasma levels in both control and ACE-inhibited spontaneously hypertensive rats, but in normalization of osmotic resistance in ACE2-inhibited rats. The overall effect of zofenopril on erythrocyte qualities could be evaluated as beneficial.
Long-Term Pharmacological Inhibition of the Activity of All NOS Isoforms Rather Than Genetic Knock-Out of Endothelial NOS Leads to Impaired Spatial Learning and Memory in C57BL/6 Mice
Authors: Hendrickx Jhana O. , De Moudt Sofie , Calus Elke , De Deyn Peter Paul , Van Dam Debby , De Meyer Guido R. Y.
Published: December 2021

Increasing epidemiological and experimental evidence points to a link between arterial stiffness and rapid cognitive decline. However, the underlying mechanism linking the two diseases is still unknown. The importance of nitric oxide synthases in both diseases is well-defined. In this study, we introduced arterial stiffness in both genetic (eNOS−/−, endothelial nitric oxide synthase knockout) and pharmacological (N(G)-nitro-L-arginine methyl ester (L-NAME) treatment) NO dysfunction models to study their association with cognitive decline. Our findings demonstrate that the non-selective inhibition of NOS activity with L-NAME induces cardiac dysfunction, arterial stiffness, and a decline in hippocampal-dependent learning and memory. This outcome demonstrates the importance of neuronal NOS (nNOS) in both cardiovascular and neurological pathophysiology and its potential contribution in the convergence between arterial stiffness and cognitive decline.
Special Issue: Pathophysiology and Therapeutic Perspectives in DMD: The Well-Defined Role of the Immune System
Authors: Farini Andrea
Published: December 2021

Duchenne muscular dystrophy (DMD) is the most common, lethal, muscle-wasting disease of childhood [...]
NASH and Systemic Complications: From Basic to Clinical Research
Authors: Baumgartner Sabine , Shiri-Sverdlov Ronit
Published: December 2021

Nonalcoholic fatty liver disease (NAFLD) is known as the hepatic manifestation of the metabolic syndrome, and while most patients develop simple steatosis, up to one-third can develop nonalcoholic steatohepatitis (NASH) [...]