bluuebunny/crossref_metadata_2025 huggingface dataset:
SONIA: an immersive customizable virtual reality system for the education and exploration of brain networks
Authors: Hellum Owen , Steele Christopher , Xiao Yiming
Published: January 2024

While mastery of neuroanatomy is important for the investigation of the brain, there is an increasing interest in exploring the neural pathways to better understand the roles of neural circuitry in brain functions. To tackle the limitations of traditional 2D-display-based neuronavigation software in intuitively visualizing complex 3D anatomies, several virtual reality (VR) and augmented reality (AR) solutions have been proposed to facilitate neuroanatomical education. However, with the increasing knowledge on brain connectivity and the functioning of the sub-systems, there is still a lack of similar software solutions for the education and exploration of these topics, which demand more elaborate visualization and interaction strategies. To address this gap, we designed the immerSive custOmizable Neuro learnIng plAtform (SONIA), a novel, user-friendly VR software system with a multi-scale interaction paradigm that allowed flexible customization of learning materials. With both quantitative and qualitative evaluations through user studies, the proposed system was shown to have high usability, attractive visual design, and good educational value. As the first immersive system that integrated customizable design and detailed narratives of the brain sub-systems for the education of neuroanatomy and brain connectivity, SONIA showcased new potential directions and provided valuable insights regarding medical learning and exploration in VR.
On the division of labor in the maintenance of intersubjectivity: insights from the study of other-initiated repair in Vietnamese
Authors: Sidnell Jack , Vũ Hương Thị Thanh
Published: January 2024

Few ideas have figured more centrally in the history of social theory than that of the division of labor. Here we ask whether conversational interaction, like other forms of social activity, exhibits a division of labor and, if so, what functions this serves and how it might be understood in relation to the theories of Marx and Durkheim. We begin by noting that, though conversational participants actively work to achieve and sustain understanding, much of the time this work is invisible and only its products are displayed in the form of sequentially fitted next turns at talk. However, in sequences of other-initiated repair, the work involved in the maintenance of intersubjectivity rises to the surface. On these occasions, we can see and thus describe what participants do to achieve and sustain what they take to be adequate understanding. In our data, which consist of video recordings of casual conversations among Vietnamese same-generation peers, participants continuously display an orientation to relations of relative seniority through the selection of terms used to accomplish interlocutor reference. This pervasive orientation is also reflected in practices of repair initiation. Specifically, seniors regularly initiate repair with so-called “open class” forms such as “huh?” and “ha?” which display a minimal grasp of the talk targeted, require little effort to produce and, at the same time, push responsibility for resolving the problem onto the trouble source speaker (i.e., the junior member of the dyad). In contrast, juniors often initiate repair of a senior participant's talk by displaying a detailed understanding of what has been said, either in the form of a repeat or a reformulation, and inviting the senior to confirm. We suggest then that this asymmetry in the distribution of initiation practices reflects a “division of intersubjective labor”. We conclude with some thoughts on the theoretical implications of our findings and relate them not only to the theories of Marx and Durkheim but also to the writings of feminist sociolinguists who sought to describe the way in which women seem to be burdened more than men with what Fishman called “interactional shitwork.”
The social return on investment of physical activity and nutrition interventions—a scoping review
Authors: Stielke Anna , Ashton Kathryn , Cotter-Roberts Andrew , Dyakova Mariana
Published: January 2024

Introduction

Scarcity of resources and mounting pressures on health systems make it critical to evaluate new and existing public health interventions related to physical activity and nutrition. The Social Return on Investment (SROI) framework has gained prominence for capturing traditional variables such as economic costs and returns, as well as wider beneficial social and environmental outcomes. A scoping review was conducted to present the existing evidence on the SROI of physical activity and nutrition interventions, demonstrating the wider benefits of these interventions.

Methodology

Existing peer-reviewed evidence and grey literature was collated to identify physical activity and nutrition interventions that were evaluated using the SROI framework between January 1996 up until February 2022. Only literature published in the English language, interventions that were conducted in high-income countries were considered for inclusion. Study information and economic data was entered into a pre-prepared data extraction sheet and eligible studies were quality assessed using a 12-point quality assessment framework for SROI studies.

Results

This review identified a total of 21 SROI studies, with only four deriving from peer-reviewed literature sources. In total, 18 studies evaluated physical activity interventions, one study was purely focused on nutrition, whereas the two remaining studies presented a mix of physical activity and nutrition. The majority of studies derived from the United Kingdom (n = 16) with very few of the studies published prior to 2010 (n = 1). In total, four studies were classified as low quality based on the 12-point quality assessment framework used for this review. Outcomes of the relevant studies show that the benefits of these interventions have added value to families, communities and the wider environments of the target groups.

Conclusion

This scoping review is adding to research conducted to understand the wider value of public health interventions such as physical activity and nutrition interventions using the SROI framework. This is important so that the development and implementation of public health interventions have the greatest value to people and society, which also benefits decision-makers to effectively and sustainably allocate scarce resources.
Spatiotemporal evolution of cropland in Northeast China’s black soil region over the past 40 years at the county scale
Authors: He Jia , Ran Duan , Tan Dong , Liao Xiaoli
Published: January 2024

This study investigates the 40-year spatiotemporal evolution of cropland in Northeast China’s black soil region at the county scale. Utilizing land use/cover maps from 1980 to 2020 with a 30 m × 30 m resolution, we employed various analytical methods, including mathematical statistics, GIS spatial analysis, land use transition matrix, landscape pattern analysis, and hotspot analysis. The findings of this study are as follows: (1) Cropland area expanded by 51,976.76 km2 from 1980 to 2020, mainly concentrated in the Sanjiang Plain, Songnen Plain, and Liaohe Plain. Notably, areas near prefecture-level city locations experienced a decrease in cropland, while regions farther from cities witnessed an increase. (2) Cropland primarily transitioned from woodland, grassland, and unused land to cropland, covering substantial areas. Conversely, cropland was converted mainly into woodland, built-up land, and grassland. (3) Over the same period, cropland in the region exhibited increased elevation and slope, with average altitude rising by 2.06 m and average slope increasing by 0.0369 degrees. (4) The study revealed an increase in cropland proportion, predominance, and aggregation, alongside more irregular shapes and reduced subdivision. These findings highlight significant changes in the cropland landscape in Northeast China’s black soil region and offer insights for policy recommendations and land management strategies. The research findings of this paper can offer valuable insights for the protection and utilization of cropland in the region. They can provide scientific references for the formulation of policies related to China’s food security.
Restricted cubic spline model analysis of the association between anal fistula and anorectal abscess incidence and body mass index
Authors: Ye Sangyu , Huang Zichen , Zheng Lihua , Shi Yuying , Zhi Congcong , Liu Ningyuan , Cheng Yicheng
Published: January 2024

Objective

The epidemiological profile of anal fistula and anorectal abscess has not been well studied. Based on the results of a retrospective cross-sectional survey, we aimed to investigate the potential influential factors associated with anal fistula and anorectal abscess.

Methods

We conducted a retrospective analysis of outpatients who visited the proctology department at China-Japan Friendship Hospital between January 2017 and May 2022. A comprehensive questionnaire was designed to collect potential influential factors, and according to formal anorectal examination and the corresponding diagnostic criteria, all the participants were divided into patients with anal fistula or perianal abscess and healthy control group. Multiple logistic regression was used to identify factors in significant association with anal fistula and perianal abscess. Additionally, we combined restricted cubic spline regression to examine the dose-response relationship between factors and the risk of developing anal fistula or anorectal abscess.

Results

The present study included 1,223 participants, including 1,018 males and 206 females, with 275 anal fistulas, 184 anorectal abscesses, and 765 healthy controls. We found no statistically significant differences between patients and controls in basic information and preoperative assessment of life factors, except for body mass index. It was indicated that people with overweight or obesity were more prone to anal fistula (OR overweight = 1.35, 95% CI: 1.00–1.82, P = 0.047; OR obesity = 3.44, 95% CI: 2.26–5.26, P < 0.001) or anorectal abscess (OR overweight = 1.41, 95% CI: 1.00–1.99, P = 0.05; OR obesity: 2.24, 95% CI: 1.37–3.67, P = 0.001) than normal-weight individuals. The dose-response research indicated the J-shaped trend between the ascending BMI levels and the higher risk of suffering from anal fistula and anorectal abscess.

Conclusions

Our findings indicate that overweight and obesity are risk factors for anal fistula and anorectal abscess, which plays a role in the prevention of anorectal diseases. This provides some theoretical basis for clinicians to provide health education to their patients.
Metabolic perturbation studies using a Nash Equilibrium model of liver machine perfusion: modeling oxidative stress and effect of glutathione supplementation
Authors: Lucia Angelo , Uygun Korkut
Published: January 2024

The current clinical standard of Static Cold Storage (SCS) which involves preservation on ice (about +4°C) in a hypoxic state limits storage to a few hours for metabolically active tissues such as the liver and the heart. This period of hypoxia during can generate superoxide and other free radicals from purine metabolism, a well-established component of ischemia/reperfusion injury (IRI). Machine perfusion is at the cutting edge of organ preservation, which provides a functional, oxygenated preservation modality that can avoid/attenuate IRI. In clinical application, perfusion usually follows a period of SCS. This presentation of oxygen following hypoxia can lead to superoxide and hydrogen peroxide generation, but machine perfusion also allows manipulation of the temperature profiles and supply of antioxidant treatments, which could be used to minimize such issues. However, metabolomic data is difficult to gather, and there are currently no mathematical models present to allow rational design of experiments or guide clinical practice. In this article, the effects of a gradual warming temperature policy and glutathione supplementation to minimize oxidative stress are studied. An optimal gradual warming temperature policy for mid-thermic machine perfusion of a liver metabolic model is determined using a combination of Nash Equilibrium and Monte Carlo optimization. Using this optimal gradual warming temperature policy, minimum GSH requirements to maintain hydrogen peroxide concentrations in the normal region are calculated using a different Monte Carlo optimization methodology. In addition, the dynamic behavior of key metabolites and cofactors are determined. Results show that the minimum GSH requirement increases and that the ratio of GSH/GSSG decreases with increasing hydrogen peroxide concentration. In addition, at high concentrations of hydrogen peroxide it is shown that cytochrome C undergoes dysfunction leading to a decrease in useful oxygen consumption and ATP synthesis from the electron transport chain and an overall reduction in the energy charge for the liver cells.
The “Big Three” in biocompatibility testing of medical devices: implementation of alternatives to animal experimentation—are we there yet?
Authors: Kanďárová Helena , Pôbiš Peter
Published: January 2024

Biocompatibility testing ensures the safety of medical devices by assessing their compatibility with biological systems and their potential to cause harm or adverse reactions. Thus, it is a critical part of the overall safety evaluation process for medical devices. Three primary types of biocompatibility tests—cytotoxicity, irritation, and sensitisation assessment—are standard for nearly all medical devices. However, additional biocompatibility tests, such as genotoxicity, systemic toxicity, hemocompatibility, and implantation studies, may also be necessary, depending on the device’s nature and intended use. The testing is partly conducted in vitro, but the industry still heavily relies on animal experiments. Compared to other industrial sectors, implementing alternatives in medical device biocompatibility testing has been notably slower. This delay can be attributed to the absence of specific validation processes tailored to medical devices and the resulting hesitation regarding the predictive capacity of these alternative methods despite their successful applications in other domains. This review focuses on the progress and obstacles to implementing new approach methodologies in the areas of cytotoxicity, irritation and sensitisation testing of medical devices. While challenges persist in adopting these innovative methods, the trend towards embracing alternatives remains robust. This trend is driven by technological advancements, ethical considerations, and growing industrial interest and support, all collectively contributing to advancing safer and more effective medical devices.
Based on CRISPR-Cas13a system, to establish a rapid visual detection method for avian influenza viruses
Authors: Zhang Zongshu , Wang Chunguang , Chen Xi , Zhang Zichuang , Shi Guoqiang , Zhai Xianghe , Zhang Tie
Published: January 2024

To rapidly, specifically, and sensitively detect avian influenza virus (AIV), this research established a visual detection method of recombinase-aided amplification (RAA) based on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR associated proteins 13a (Cas13a) system. In this study, specific primers and CRISPR RNA (crRNA) were designed according to the conservative sequence of AIV Nucleprotein (NP) gene. RAA technology was used to amplify the target sequence, and the amplification products were visually detected by lateral flow dipstick (LFD). The specificity, sensitivity, and reproducibility of RAA-CRISPR-Cas13a-LFD were evaluated. At the same time, this method and polymerase chain reaction (PCR)-agarose electrophoresis method were used to detect clinical samples, and the coincidence rate of the two detection methods was calculated. The results showed that the RAA-CRISPR-Cas13a-LFD method could achieve specific amplification of the target gene fragments, and the detection results could be visually observed through the LFD. Meanwhile, there was no cross-reaction with infectious bronchitis virus (IBV), infectious laryngotracheitis virus (ILTV), and Newcastle disease virus (NDV). The sensitivity reached 100 copies/μL, which was 1,000-fold higher than that of PCR-agarose electrophoresis method. The coincidence rate of clinical tests was 98.75 %, and the total reaction time was ~1 h. The RAA-CRISPR-Cas13a-LFD method established in this study had the advantages of rapid, simple, strong specificity, and high sensitivity, which provided a new visual method for AIV detection.
Has avian influenza virus H9 originated from a bat source?
Authors: Karamendin Kobey , Kydyrmanov Aidyn , Fereidouni Sasan
Published: January 2024

Influenza A viruses are important pathogens that can cause diseases with high mortality in humans, animals, and birds; and wild birds are considered the primary reservoir of all subtypes in nature. After discovering the H9 influenza A viruses in bats, questions arose about their potential to serve as an additional natural reservoir and about the priority of the viral origin: Did the virus initially circulate in bats and then transmit to birds or vice versa? Influenza A viruses of the H9 subtype are of particular interest because fatal infections of humans caused by H5, H7, and H10 influenza viruses contained RNA segments from H9 viruses. Recently, a novel subtype of influenza A virus (H19) was reported and it was closely related to the H9 bat influenza A virus by its hemagglutinin structure. The genome of novel H19 has revealed a mixed characteristic genomic signature of both avian and bat influenza viruses. The time to most recent common ancestor (TMRCA) estimates have shown that the divergence time between the bat and avian H9-similar influenza virus occurred approximately at the end of the XVIII century. This article discusses the evolution and possible origin of influenza viruses of the H9 subtype isolated from bats and birds. The obtained data, along with the known data, suggest that the primary reservoir of the H9 influenza virus is wild birds, from which the virus was transmitted to bats. We hypothesize that the novel H19 could be a descendant of an intermediate influenza virus that was in the transition stage of spillover from avian to bat hosts.
Active walking in broiler chickens: a flagship for good welfare, a goal for smart farming and a practical starting point for automated welfare recognition
Authors: Dawkins Marian Stamp
Published: January 2024

Automated assessment of broiler chicken welfare poses particular problems due to the large numbers of birds involved and the variety of different welfare measures that have been proposed. Active (sustained, defect-free) walking is both a universally agreed measure of bird health and a behavior that can be recognized by existing technology. This makes active walking an ideal starting point for automated assessment of chicken welfare at both individual and flock level.
Differential susceptibility of human microglia HMC3 cells and brain microvascular endothelial HBEC-5i cells to Mayaro and Una virus infection
Authors: Campos Dalkiria , Sugasti-Salazar Madelaine , Valdés-Torres Patricia , Galán-Jurado Paola Elaine , Zegarra Dalel , González-Santamaría José
Published: January 2024

Mayaro (MAYV) and Una (UNAV) are emerging alphaviruses circulating in the Americas. Earlier reports have revealed that MAYV infects different human cell lines, including synovial and dermal fibroblasts, chondrocytes, osteoblasts, astrocytes and pericytes, as well as neural progenitor cells. In this study we evaluated the susceptibility of immortalized human microglia HMC3 cells and brain microvascular endothelial HBEC-5i cells to MAYV and UNAV infection. Cytopathic effects, cell viability, viral progeny yields, and the presence of E1 and nsP1 proteins in HMC3 and HBEC-5i cells infected with several MAYV or UNAV strains were assessed using an inverted microscope, MTT assay, plaque-forming assays, and immunofluorescence or Western blot, respectively. Finally, the expression of immune response genes was analyzed using RT-qPCR. MAYV and UNAV demonstrated strong cytopathic effects and significantly reduced cell viability in HMC3 cells. Moreover, the HMC3 cells were efficiently infected regardless of the virus strain tested, and E1 and nsP1 viral proteins were detected. In contrast, only MAYV appeared to infect HBEC-5i cells, and minimal effects on cell morphology or viability were observed. Furthermore, the MAYV titer and viral protein levels were substantially lower in the infected HBEC-5i cells when compared to those of the infected microglia cells. Finally, unlike UNAV, MAYV elicited a strong expression of specific interferon-stimulated genes in microglia cells, along with pro-inflammatory cytokines implicated in the immune response. Collectively, these findings demonstrate that MAYV and UNAV are capable of infecting relevant human brain cells.
Particle Swarm Optimization-Based Unconstrained Polygonal Fitting of 2D Shapes
Authors: Panagiotakis Costas
Published: January 2024

In this paper, we present a general version of polygonal fitting problem called Unconstrained Polygonal Fitting (UPF). Our goal is to represent a given 2D shape S with an N-vertex polygonal curve P with a known number of vertices, so that the Intersection over Union (IoU) metric between S and P is maximized without any assumption or prior knowledge of the object structure and the location of the N-vertices of P that can be placed anywhere in the 2D space. The search space of the UPF problem is a superset of the classical polygonal approximation (PA) problem, where the vertices are constrained to belong in the boundary of the given 2D shape. Therefore, the resulting solutions of the UPF may better approximate the given curve than the solutions of the PA problem. For a given number of vertices N, a Particle Swarm Optimization (PSO) method is used to maximize the IoU metric, which yields almost optimal solutions. Furthermore, the proposed method has also been implemented under the equal area principle so that the total area covered by P is equal to the area of the original 2D shape to measure how this constraint affects IoU metric. The quantitative results obtained on more than 2800 2D shapes included in two standard datasets quantify the performance of the proposed methods and illustrate that their solutions outperform baselines from the literature.
Design of Convergent and Accurate Guidance Law with Finite Time in Complex Adversarial Scenarios
Authors: Ding Hang , Wang Dongdong , Li Chuanjun , Liang Xiao , Li Xingcheng
Published: January 2024

The target can deceive the flight vehicle by releasing an infrared decoy to make the line-of-sight (LOS) angle rate deflect greatly, thus causing the flight vehicle to miss the target. Therefore, in order to accurately strike the target in complex adversarial scenarios, this paper proposes a finite-time convergence guidance law (FTCG) combined with a finite-time disturbance observer (FTDO). The complex adversarial scenario is established by combining the relative motion model between the flight vehicle and the target and the motion model of the infrared decoy. Based on this, considering the dynamic characteristic of the flight vehicle’s autopilot, a guidance model is obtained. Utilizing sliding mode control theory and finite-time control theory, an FTCG of the LOS angle rate is designed. Then, the finite-time convergence of the guidance law is proved and the total convergence time is derived. Finally, for the target maneuvering that is difficult to measure in the guidance law, an FTDO is used to estimate and compensate for the target maneuvering in the guidance law. Simulation results show that the FTCG can make the LOS angle rate quickly converge and accurately strike the target in different scenarios, with a good guidance accuracy and robustness. Compared with the sliding mode guidance law (SMGL) and the adaptive sliding mode guidance law (ASMGL) based on an extended state observer (ESO), the advantages of the designed guidance law are illustrated. Finally, FTCG is extended to be three dimensional and compared with the proportional navigation guidance law (PNG) to further illustrate its effectiveness in a three-dimensional coordinate system.
Real Gas Effects on Receptivity to Roughness in Hypersonic Swept Blunt Flat-Plate Boundary Layers
Authors: Yin Yanxin , Lu Ruiyang , Liu Jianxin , Huang Zhangfeng
Published: January 2024

Temperatures within the boundary layers of high-enthalpy hypersonic flows can soar to thousands or even tens of thousands of degrees, leading to significant real gas phenomena. Although there has been significant research on real gas effects on hypersonic boundary layer stability, their impact on the boundary layer’s receptive stage is still poorly understood. Most aerodynamic boundary layers in flight vehicles are three-dimensional. Because of complex geometry and significant crossflow effects, the crossflow mode in three-dimensional boundary layers is crucial in hypersonic vehicle design. In this study, a linear stability analysis (LST) accounting for chemical nonequilibrium effects (CNE) and its adjoint form (ALST) is developed to investigate the real gas effects on the stability and receptivity of stationary crossflow modes. The results indicate that real gas effects significantly influence the receptivity of stationary crossflow modes. Specifically, chemical nonequilibrium effects destabilize the crossflow modes but reduce the receptivity coefficients of the stationary crossflow modes. The Mach number effect was also investigated. It was found that increasing the Mach number stabilizes the stationary crossflow modes, but the receptivity coefficients increase. As the Mach number progressively rises, these effects alternately dominate, leading to a non-monotonic shift in the transition position.
Consequences of Transport Conditions on the Welfare of Slaughter Pigs with Different Health Status and RYR-1 Genotype
Authors: Čobanović Nikola , Čalović Sara , Suvajdžić Branko , Grković Nevena , Stanković Sanja Dj , Radaković Milena , Spariosu Kristina , Karabasil Nedjeljko
Published: January 2024

This study assessed the influence of transport conditions on welfare indicators of slaughter pigs with different health status and RYR-1 genotype. The group of pigs, predominantly consisting of Nn (56.67%) and subclinically diseased (60.00%) individuals, that were exposed to short transportation (<30 min) at high loading density (~235 kg/m2) had the highest slipping (p < 0.0001), falling (p = 0.0009), turning back (p < 0.0001), reluctance to move (p < 0.0001), panting (p < 0.0001) and shivering (p < 0.0001) frequencies at unloading. Subclinically diseased Nn pigs subjected to short transportation (<30 min) and high loading density (~235 kg/m2) had the highest lactate (p < 0.0001 and p < 0.0001), glucose (p = 0.0450 and p = 0.0002), CK (p < 0.0001 and p = 0.0010), LDH (p < 0.0001 and p = 0.0484), AST (p = 0.0208 and p = 0.0170), ALT (p = 0.0500 and p = 0.00081), ceruloplasmin (p = 0.0334 and p < 0.0001) and MDA (p = 0.0048 and p < 0.0001) concentrations, but the lowest sodium (p < 0.0001 and p < 0.0001), chloride (p = 0.0001 and p = 0.0432), albumin (p < 0.0090 and p < 0.0001), PON-1 (p = 0.0122 and p = 0.0500) and GSH (p = 0.0042 and p = 0.0340) levels, respectively. In the group consisting of of stress-resistant (100%) and predominantly healthy (60.00%) pigs subjected to short transportation (<30 min) at high loading density (~235 kg/m2), none of the individuals showed irregular behavioural reactions during unloading. Healthy NN pigs that underwent short transportation (<30 min) at high loading density (~235 kg/m2) had the lowest lactate (p < 0.0001 and p < 0.0001), glucose (p = 0.0450 and p = 0.0002), CK (p < 0.0001 and p = 0.0010), LDH (p < 0.0001 and p = 0.0484) and ceruloplasmin (p = 0.0334 and p < 0.0001) levels, but the highest sodium (p < 0.0001 and p < 0.0001) and chloride (p = 0.0001 and p = 0.0432) concentrations, respectively. In conclusion, the most compromised welfare was recorded in subclinically diseased Nn pigs exposed to short transportation (<30 min) and high loading density (~235 kg/m2), while under the same conditions, the welfare of healthy NN pigs was not compromised. Therefore, stress-carrier pigs with subclinical pathological lesions should not be considered fit for transportation, indicating that the health status and genotype are the key factors for optimising pig welfare.
Can Nicotinamide Adenine Dinucleotide (NAD+) and Sirtuins Be Harnessed to Improve Mare Fertility?
Authors: Pollard Charley-Lea
Published: January 2024

Years of sire and dam selection based on their pedigree and athletic performance has resulted in a reduction in the reproductive capability of horses. Mare age is considered a major barrier to equine reproduction largely due to an increase in the age at which mares are typically bred following the end of their racing career. Nicotinamide adenine dinucleotide (NAD+) and its involvement in the activation of Sirtuins in fertility are an emerging field of study, with the role of NAD+ in oocyte maturation and embryo development becoming increasingly apparent. While assisted reproductive technologies in equine breeding programs are in their infancy compared to other livestock species such as cattle, there is much more to be learnt, from oocyte maturation to early embryo development and beyond in the mare, which are difficult to study given the complexities associated with mare fertility research. This review examines what is already known about the role of NAD+ and Sirtuins in fertility and discusses how NAD+-elevating agents may be used to activate Sirtuin proteins to improve equine breeding and embryo production programs both in vivo and in vitro.
Comparative Blood Transcriptome Analysis of Semi-Natural and Controlled Environment Populations of Yangtze Finless Porpoise
Authors: Liu Wang , Yin Denghua , Li Zhanwei , Zhu Xiaoyan , Zhang Sigang , Zhang Peng , Lin Danqing , Hua Zhong , Cao Zhichen , Zhang Han , Zhang Jialu , Ying Congping , Xu Pao , Dong Guixin , Liu Kai
Published: January 2024

The Yangtze finless porpoises (Neophocaena asiaeorientalis asiaeorientalis) living in different environments display significant differences in behavior and physiology. To compare and analyze gene expression differences between an ex situ population and a controlled environment population of the Yangtze finless porpoise, we sequenced the transcriptome of blood tissues living in a semi-natural reserve and an artificial facility, respectively. We identified 6860 differentially expressed genes (DEGs), of which 6603 were up-regulated and 257 were down-regulated in the controlled environment vs ex situ comparison. GO and KEGG enrichment analysis showed that the up-regulated genes in the controlled environment population were significantly associated with glucose metabolism, amino acid metabolism, and the nervous system, while those up-regulated in the ex situ population were significantly associated with energy supply and biosynthesis. Further analysis showed that metabolic and hearing-related genes were significantly affected by changes in the environment, and key metabolic genes such as HK, PFK, IDH, and GLS and key hearing-related genes such as OTOA, OTOF, SLC38A1, and GABBR2 were identified. These results suggest that the controlled environment population may have enhanced glucose metabolic ability via activation of glycolysis/gluconeogenesis, the TCA cycle, and inositol phosphate metabolism, while the ex situ population may meet higher energy requirements via enhancement of the amino acid metabolism of the liver and muscle and oxidative phosphorylation. Additionally, the acoustic behavior and auditory-related genes of Yangtze finless porpoise may show responsive changes and differential expression under different environment conditions, and thus the auditory sensitivity may also show corresponding adaptive characteristics. This study provides a new perspective for further exploration of the responsive changes of the two populations to various environments and provides a theoretical reference for further improvements in conservation practices for the Yangtze finless porpoise.
New Antibiotics for the Treatment of Nosocomial Central Nervous System Infections
Authors: Nau Roland , Seele Jana , Eiffert Helmut
Published: January 2024

Nosocomial central nervous system (CNS) infections with carbapenem- and colistin-resistant Gram-negative and vancomycin-resistant Gram-positive bacteria are an increasing therapeutic challenge. Here, we review pharmacokinetic and pharmacodynamic data and clinical experiences with new antibiotics administered intravenously for the treatment of CNS infections by multi-resistant bacteria. Cefiderocol, a new siderophore extended-spectrum cephalosporin, pharmacokinetically behaves similar to established cephalosporins and at high doses will probably be a valuable addition in our therapeutic armamentarium for CNS infections. The new glycopeptides dalbavancin, telavancin, and oritavancin are highly bound to plasma proteins. Although effective in animal models of meningitis, it is unlikely that they reach effective cerebrospinal fluid (CSF) concentrations after intravenous administration alone. The β-lactam/β-lactamase inhibitor combinations have the principal problem that both compounds must achieve adequate CSF concentrations. In the commercially available combinations, the dose of the β-lactamase inhibitor tends to be too low to achieve adequate CSF concentrations. The oxazolidinone tedizolid has a broader spectrum but a less suitable pharmacokinetic profile than linezolid. The halogenated tetracycline eravacycline does not reach CSF concentrations sufficient to treat colistin-resistant Gram-negative bacteria with usual intravenous dosing. Generally, treatment of CNS infections should be intravenous, whenever possible, to avoid adverse effects of intraventricular therapy (IVT). An additional IVT can overcome the limited penetration of many new antibiotics into CSF. It should be considered for patients in which the CNS infection responds poorly to systemic antimicrobial therapy alone.
Design, Validation and CFD Modeling of an Environmental Wind Tunnel
Authors: Pinna Francesco , Grosso Battista , Lai Alessio , Bouarour Ouiza , Armas Cristiano , Serci Maurizio , Dentoni Valentina
Published: January 2024

The wind erosion of granular materials stored within the open yards of industrial plants (i.e., industrial wind erosion) and the subsequent emission and dispersion of particulate matter (PM) in the surrounding areas represent an important issue for the exposed population and for the environment as a whole. The Environment Impact Assessment (EIA) and the design of emission control measures require a deep knowledge of the erosion phenomenon, and the precise estimation of the Emission Factors (EF) associated with the specific PM source under investigation. Aiming to characterize the emission potential of industrial granular materials, a new Environmental Wind Tunnel (EWT) has recently been built at the Department of Civil and Environmental Engineering and Architecture (DICAAR) laboratories, in Cagliari University. The article discusses the EWT’s updated design and the set-up methodologies applied to reproduce the Atmospheric Boundary Layer (ABL) acting over the surfaces of coarse and heterogeneous granular materials. In addition, a Computational Fluid Dynamics (CFD) model of the EWT has been developed to reproduce and analyze the wind field throughout the entire tunnel volume and preliminarily evaluate possible modifications to the original design. The accuracy of the simulation has been verified by comparing the CFD model and the results of the experimental tests.
Pilot Scale Continuous Pulsed Electric Fields Treatments for Vinification and Stabilization of Arinto and Moscatel Graúdo (Vitis vinifera L.) White Grape Varieties: Effects on Sensory and Physico-Chemical Quality of Wines
Authors: Silva Filipa Vinagre Marques , Borgo Riccardo , Guanziroli Andrea , Ricardo-da-Silva Jorge M. , Aguiar-Macedo Mafalda , Redondo Luis M.
Published: January 2024

Pulsed electric field (PEF) processing of white grapes (Arinto, Moscatel Graúdo also known as Moscatel de Setúbal) before pressing for must extraction (1.2 and 1.6 kV/cm) and before bottling for finished wine physical stabilization (10 kV/cm) was implemented in a pilot-scale winery to produce about 540 L of wine for each variety. PEF was applied at these two different stages of wine production, and its effects on the sensory and physico-chemical quality of the wines were investigated. The sensory triangle tests revealed no significant change in both wine varieties’ colour, odour, and taste with PEF extraction and PEF stabilization treatments. However, for colour coordinates assessed with a spectrophotometer, a significant increase in CIE b* colour coordinate was registered for PEF-extracted Arinto and Moscatel wine samples, showing a development of a more intense yellow colour. Concerning physico-chemical quality parameters, the PEF extraction increased both wine varieties’ turbidity and pH, although total acidity was not affected. The total phenols also increased in Arinto with extraction. The second PEF treatment applied for wine stabilization did not affect any of the quality parameters, except total phenols, which decreased in Moscatel wine. The results encourage the application of PEF in the wineries at different stages of vinification of white wine grape varieties.
ChatGPT in Occupational Medicine: A Comparative Study with Human Experts
Authors: Padovan Martina , Cosci Bianca , Petillo Armando , Nerli Gianluca , Porciatti Francesco , Scarinci Sergio , Carlucci Francesco , Dell’Amico Letizia , Meliani Niccolò , Necciari Gabriele , Lucisano Vincenzo Carmelo , Marino Riccardo , Foddis Rudy , Palla Alessandro
Published: January 2024

The objective of this study is to evaluate ChatGPT’s accuracy and reliability in answering complex medical questions related to occupational health and explore the implications and limitations of AI in occupational health medicine. The study also provides recommendations for future research in this area and informs decision-makers about AI’s impact on healthcare. A group of physicians was enlisted to create a dataset of questions and answers on Italian occupational medicine legislation. The physicians were divided into two teams, and each team member was assigned a different subject area. ChatGPT was used to generate answers for each question, with/without legislative context. The two teams then evaluated human and AI-generated answers blind, with each group reviewing the other group’s work. Occupational physicians outperformed ChatGPT in generating accurate questions on a 5-point Likert score, while the answers provided by ChatGPT with access to legislative texts were comparable to those of professional doctors. Still, we found that users tend to prefer answers generated by humans, indicating that while ChatGPT is useful, users still value the opinions of occupational medicine professionals.
Fiber-Optic Pedicle Probes to Advance Spine Surgery through Diffuse Reflectance Spectroscopy
Authors: Losch Merle S. , Heintz Justin D. , Edström Erik , Elmi-Terander Adrian , Dankelman Jenny , Hendriks Benno H. W.
Published: January 2024

Diffuse Reflectance Spectroscopy (DRS) can provide tissue feedback for pedicle screw placement in spine surgery, yet the integration of fiber optics into the tip of the pedicle probe, a device used to pierce through bone, is challenging, since the optical probing depth and signal-to-noise ratio (SNR) are affected negatively compared to those of a blunt DRS probe. Through Monte Carlo simulations and optical phantom experiments, we show how differences in the shape of the instrument tip influence the acquired spectrum. Our findings demonstrate that a single bevel with an angle of 30∘ offers a solution to anticipate cortical breaches during pedicle screw placement. Compared to a blunt probe, the optical probing depth and SNR of a cone tip are reduced by 50%. The single bevel tip excels with 75% of the optical probing depth and a SNR remaining at approximately ⅔, facilitating the construction of a surgical instrument with integrated DRS.
MicroRNAs Associated with a Bad Prognosis in Acute Myeloid Leukemia and Their Impact on Macrophage Polarization
Authors: Jimbu Laura , Mesaros Oana , Joldes Corina , Neaga Alexandra , Zaharie Laura , Zdrenghea Mihnea
Published: January 2024

MicroRNAs (miRNAs) are short, non-coding ribonucleic acids (RNAs) associated with gene expression regulation. Since the discovery of the first miRNA in 1993, thousands of miRNAs have been studied and they have been associated not only with physiological processes, but also with various diseases such as cancer and inflammatory conditions. MiRNAs have proven to be not only significant biomarkers but also an interesting therapeutic target in various diseases, including cancer. In acute myeloid leukemia (AML), miRNAs have been regarded as a welcome addition to the limited therapeutic armamentarium, and there is a vast amount of data on miRNAs and their dysregulation. Macrophages are innate immune cells, present in various tissues involved in both tissue repair and phagocytosis. Based on their polarization, macrophages can be classified into two groups: M1 macrophages with pro-inflammatory functions and M2 macrophages with an anti-inflammatory action. In cancer, M2 macrophages are associated with tumor evasion, metastasis, and a poor outcome. Several miRNAs have been associated with a poor prognosis in AML and with either the M1 or M2 macrophage phenotype. In the present paper, we review miRNAs with a reported negative prognostic significance in cancer with a focus on AML and analyze their potential impact on macrophage polarization.
Behavioral and Cognitive Comorbidities in Genetic Rat Models of Absence Epilepsy (Focusing on GAERS and WAG/Rij Rats)
Authors: Sitnikova Evgenia
Published: January 2024

Absence epilepsy is a non-convulsive type of epilepsy characterized by the sudden loss of awareness. It is associated with thalamo-cortical impairment, which may cause neuropsychiatric and neurocognitive problems. Rats with spontaneous absence-like seizures are widely used as in vivo genetic models for absence epilepsy; they display behavioral and cognitive problems similar to epilepsy in humans, such as genetic absence epilepsy rats from Strasbourg (GAERS) and Wistar Albino rats from Rijswijk (WAG/Rij). Both GAERS and WAG/Rij rats exhibited depression-like symptoms, but there is uncertainty regarding anxiety-related symptoms. Deficits in executive functions and memory impairment in WAG/Rij rats, i.e., cognitive comorbidities, are linked to the severity of epilepsy. Wistar rats can develop spontaneous seizures in adulthood, so caution is advised when using them as a control epileptic strain. This review discusses challenges in the field, such as putative high emotionality in genetically prone rats, sex differences in the expression of cognitive comorbidities, and predictors of cognitive problems or biomarkers of cognitive comorbidities in absence epilepsy, as well as the concept of “the cognitive thalamus”. The current knowledge of behavioral and cognitive comorbidities in drug-naive rats with spontaneous absence epilepsy is beneficial for understanding the pathophysiology of absence epilepsy, and for finding new treatment strategies.
Involvement of Lysophospholipids in Pulmonary Vascular Functions and Diseases
Authors: Kume Hiroaki , Harigane Rina , Rikimaru Mami
Published: January 2024

Extracellular lysophospholipids (lysophosphatidic acid, lysophosphatidylcholine, sphingosine 1-phosphate, etc.), which are synthesized from phospholipids in the cell membrane, act as lipid mediators, and mediate various cellular responses in constituent cells in the respiratory system, such as contraction, proliferation, migration, and cytoskeletal organization. In addition to these effects, the expression of the adhesion molecules is enhanced by these extracellular lysophospholipids in pulmonary endothelial cells. These effects are exerted via specific G protein-coupled receptors. Rho, Ras, and phospholipase C (PLC) have been proven to be their signaling pathways, related to Ca2+ signaling due to Ca2+ dynamics and Ca2+ sensitization. Therefore, lysophospholipids probably induce pulmonary vascular remodeling through phenotype changes in smooth muscle cells, endothelial cells, and fibroblasts, likely resulting in acute respiratory distress syndrome due to vascular leak, pulmonary hypertension, and pulmonary fibrosis. Moreover, lysophospholipids induce the recruitment of inflammatory cells to the lungs via the enhancement of adhesion molecules in endothelial cells, potentially leading to the development of asthma. These results demonstrate that lysophospholipids may be novel therapeutic targets not only for injury, fibrosis, and hypertension in the lung, but also for asthma. In this review, we discuss the mechanisms of the effects of lysophospholipids on the respiratory system, and the possibility of precision medicine targeting lysophospholipids as treatable traits of these diseases.
The Effects of Distancing Design Collaboration Necessitated by COVID-19 on Brain Synchrony in Teams Compared to Co-Located Design Collaboration: A Preliminary Study
Authors: Shih Yi-Teng , Wang Luqian , Wong Clive H. Y. , Sin Emily L. L. , Rauterberg Matthias , Yuan Zhen , Chang Leanne
Published: January 2024

Due to the widespread involvement of distributed collaboration triggered by COVID-19, it has become a new trend that has continued into the post-pandemic era. This study investigated collective performance within two collaborative environments (co-located and distancing settings) by assessing inter-brain synchrony patterns (IBS) among design collaborators using functional near-infrared spectroscopy. The preliminary study was conducted with three dyads who possessed 2–3 years of professional product design experience. Each dyad completed two designated design tasks in distinct settings. In the distributed condition, participants interacted through video conferencing in which they were allowed to communicate by verbalization and sketching using a shared digital whiteboard. To prevent the influences of different sketching tools on design outputs, we employed digital sketching for both environments. The interactions between collaborators were identified in three behaviors: verbal only, sketch only, and mixed communication (verbal and sketch). The consequences revealed a higher level of IBS when mixed communication took place in distributed conditions than in co-located conditions. Comparably, the occurrence of IBS increased when participants solely utilized sketching as the interaction approach within the co-located setting. A mixed communication method combining verbalization and sketching might lead to more coordinated cognitive processes when in physical isolation. Design collaborators are inclined to adjust their interaction behaviors in order to adapt to different design environments, strengthen the exchange of ideas, and construct design consensus. Overall, the present paper discussed the performance of virtual collaborative design based on a neurocognitive perspective, contributing valuable insights for the future intervention design that promotes effective virtual teamwork.