bluuebunny/crossref_metadata_2025 huggingface dataset:
Removal of the syndesmotic screw after the surgical treatment of a fracture of the ankle in adult patients does not affect one-year outcomes
Authors: Boyle M. J. , Gao R. , Frampton C. M. A. , Coleman B.
Published: December 2014

Our aim was to compare the one-year post-operative outcomes following retention or removal of syndesmotic screws in adult patients with a fracture of the ankle that was treated surgically. A total of 51 patients (35 males, 16 females), with a mean age of 33.5 years (16 to 62), undergoing fibular osteosynthesis and syndesmotic screw fixation, were randomly allocated to retention of the syndesmotic screw or removal at three months post-operatively. The two groups were comparable at baseline.

One year post-operatively, there was no significant difference in the mean Olerud–Molander ankle score (82.4 retention vs 86.7 removal, p = 0.367), the mean American Orthopedic Foot and Ankle Society ankle-hindfoot score (88.6 vs 90.1, p = 0.688), the mean American Academy of Orthopedic Surgeons foot and ankle score (96.3 vs 94.0, p = 0.250), the mean visual analogue pain score (1.0 vs 0.7, p = 0.237), the mean active dorsiflexion (10.2° vs 13.0°, p = 0.194) and plantar flexion (33.6° vs 31.3°, p = 0.503) of the ankle, or the mean radiological tibiofibular clear space (5.0 mm vs 5.3 mm, p = 0.276) between the two groups. A total of 19 patients (76%) in the retention group had a loose and/or broken screw one year post-operatively.

We conclude that removal of a syndesmotic screw produces no significant functional, clinical or radiological benefit in adult patients who are treated surgically for a fracture of the ankle.

Cite this article: Bone Joint J 2014;96-B:1699–1705.
Study on Salting out-Steam Distillation Extraction Technology and Antibacterial Activities of Essential Oil from Cumin Seeds
Authors: Zhang Hong , Hu Qing-Ping
Published: November 2014

The effects of different factors on the yield of essential oil from were discussed, and the extraction conditions of essential oil from cumin seeds by salting out-steam distillation technology based on single-factor test and orthogonal experiment, as well as its antibacterial activities on several common food spoilage bacteria were studied in this paper. The results showed that, the impact order of the influence factors was liquid/solid ratio distilling time NaCl concentration, and optimized extraction conditions were as follows, liquid to material ratio 15:1, soaking time 1 h, 4% NaCl, steam distilling time 3 h. The yield of essential oil was up to 4.48% under these conditions. The results of antibacterial activity assays showed that the essential oil from cumin seeds exhibited the different antibacterial activities against some food borne pathogens, especially it presented the best inhibitory effect against Bacillus subtilis with the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of 6.25 and 12.5 mg/mL respectively, followed by Staphylococcus albus and Staphylococcus aureus, the lowest for Pseudomonas aeruginosa and Shigella dysenteriae
Evaluation of In-Line Raman Spectroscopic Monitoring of Size Reduction during Wet Media Milling of Biopharmaceutics Classification System Class II Drugs
Authors: Ying Ye , Afolabi Afolawemi , Bilgili Ecevit , Iqbal Zafar
Published: November 2014

The aim of this study was to evaluate in-line Raman spectroscopy for monitoring the progress of particle size reduction in real time during wet-stirred media milling of two Biopharmaceutics Classification System (BCS) Class II drugs, griseofulvin and naproxen. To develop a validated online Raman method, Raman analyses were carried out offline by taking samples from the mill at various milling times. A multivariate linear model (partial least squares, PLS) was fitted to the raw data obtained from the Raman measurements and good linearity between online and offline Raman spectra was found. Line intensities (I) of the in-line spectra obtained during the wet media milling were processed by multivariate analyses and correlated with both the median size (d50) and the 90% passing particle size (d90 ), which were measured offline by laser diffraction. A two-parameter exponential growth model of the form d = exp[ A(I – I0)] was found to establish a good correlation ( R 2 > 0.90) as a statistically significant model with statistically significant parameters ( P < 10−4). The correlations were applicable to milled suspensions with particles in the approximate size range of 0.1–6 μm for griseofulvin and 0.1–8 μm for naproxen. These results suggest that in-line Raman spectroscopy can be used to successfully monitor the progress of particle size reduction during wet media milling.
Diffuse Reflectance Spectroscopy as a Possible Tool to Complement Liver Biopsy for Grading Hepatic Fibrosis in Paraffin-Preserved Human Liver Specimens
Authors: Fabila-Bustos Diego A. , Arroyo-Camarena Ursula D. , López-Vancell María D. , Durán-Padilla Marco A. , Azuceno-García Itzel , Stolik-Isakina Suren , Ibarra-Coronado Elizabeth , Brown Blair , Escobedo Galileo , de la Rosa-Vázquez José Manuel
Published: November 2014

A diffuse reflectance spectroscopy-based method to score fibrosis in paraffin-preserved human liver specimens has been developed and is reported here. Paraffin blocks containing human liver tissue were collected from the General Hospital of Mexico and included in the study with the patients' written consent. The score of liver fibrosis was determined in each sample by two experienced pathologists in a single-blind fashion. Spectral measurements were acquired at 450–750 nm by establishing surface contact between the optical probe and the preserved tissue. According to the histological evaluation, four liver samples showed no evidence of fibrosis and were categorized as F0, four hepatic specimens exhibited an initial degree of fibrosis (F1—F2), five liver specimens showed a severe degree of fibrosis (F3), and six samples exhibited cirrhosis (F4). The human liver tissue showed a characteristic diffuse reflectance spectrum associated with the progressive stages of fibrosis. In the F0 liver samples, the diffuse reflection intensity gradually increased in the wavelength range of 450–750 nm. In contrast, the F1–F2, F3, and F4 specimens showed corresponding 1.5-, 2-, and 5.5-fold decreases in the intensity of diffuse reflectance compared to the F0 liver specimens. At 650 nm, all the stages of liver fibrosis were clearly distinguished from each other with high sensitivity and specificity (92–100%). To our knowledge, this is the first study reporting a distinctive diffuse reflectance spectrum for each stage of fibrosis in paraffin-preserved human liver specimens. These results suggest that diffuse reflectance spectroscopy may represent a complementary tool to liver biopsy for grading fibrosis.
Investigating Lignin Key Features in Maize Lignocelluloses Using Infrared Spectroscopy
Authors: Chazal Richard , Robert Paul , Durand Sylvie , Devaux Marie-Françoise , Saulnier Luc , Lapierre Catherine , Guillon Fabienne
Published: November 2014

Lignins and their cross-linking to hemicelluloses detrimentally affect the cellulose-to-ethanol conversion of grass lignocelluloses. Screening appropriate grass cell walls and their compositional changes during the various steps of the process calls for a high-throughput analytical technique. Such a performance can be fulfilled by Fourier transform mid-infrared (FT-MIR) spectroscopy. In the present paper, a set of maize cell walls from mature stems were selected, including brown midrib samples. Lignin fractions were isolated by mild acidolysis to obtain a set of purified maize lignin standards. The lignin content and the percentage of lignin-derived p-hydroxyphenyl (H), guaiacyl (G), and syringyl (S) thioacidolysis monomers were determined. In addition, the composition of cell wall polysaccharides, as well as the amount of ester-linked p-coumaric (CA) and ferulic (FA) acids, was measured by wet chemistry. Partial least square (PLS) analyses were applied to infrared and chemical data of cell walls. The resulting models showed a good predictive ability with regard to the lignin content, to the frequency of S (or G) thioacidolysis monomers, and to the level of ester-linked CA of maize cell walls. The loading plots and regression coefficients revealed relevant infrared absorption bands.
Substance use avoidance among Iranian male adolescents: a comparison of three versions of the theory of reasoned action
Authors: Tavousi Mahmoud , Montazeri Ali , Hidarnia Alireza , Hajizadeh Ebrahim , Taremian Farhad , Haerimehrizi Aliasghar
Published: November 2014

The theory of reasoned action (TRA) is one of the most common models in predicting health-related behaviors and is used more often in health education studies. This study aimed to add two control constructs (perceived behavioral control – PBC and self-efficacy – SE) to the TRA and compare them using the structural equation modeling (SEM) for substance use avoidance among Iranian male adolescents in order to find out which model was a better fit in predicting the intention. This was a cross-sectional study carried out in Tehran, Iran. Data were collected from a random sample of high school male students (15–19 years of age) using a questionnaire containing items related to the TRA plus items reflecting two additional constructs (SE and PBC). In all, 433 students completed the questionnaires. The results obtained from SEM indicated a better fit to the data for the TRA with SE compared to the TPB (TRA with PBC) and TRA (χ2/df=2.55, RMSEA=0.072, CFI=0.96, NFI=0.94, NNFI=0.95, SRMR=0.058). Comparing SE and PBC, the results showed that self-efficacy was a better control construct in improving the TRA and predicting substance use avoidance intention (41%). The TRA with SE had a better model fit than TPB and the original version of the TRA.
Equilibrium equations and boundary conditions of strain gradient theory in arbitrary curvilinear coordinates
Authors: Guzev Mikhail , Qi Chengzhi , Bai Jiping , Li Kairui
Published: December 2014

Equilibrium equations and boundary conditions of the strain gradient theory in arbitrary curvilinear coordinates have been obtained. Their special form for an axisymmetric plane strain problem is also given.
A Competitive Advantage by Neonatally Engrafted Human Glial Progenitors Yields Mice Whose Brains Are Chimeric for Human Glia
Authors: Windrem Martha S. , Schanz Steven J. , Morrow Carolyn , Munir Jared , Chandler-Militello Devin , Wang Su , Goldman Steven A.
Published: November 2014

Neonatally transplanted human glial progenitor cells (hGPCs) densely engraft and myelinate the hypomyelinatedshiverermouse. We found that, in hGPC-xenografted mice, the human donor cells continue to expand throughout the forebrain, systematically replacing the host murine glia. The differentiation of the donor cells is influenced by the host environment, such that more donor cells differentiated as oligodendrocytes in the hypomyelinatedshivererbrain than in myelin wild-types, in which hGPCs were more likely to remain as progenitors. Yet in each recipient, both the number and relative proportion of mouse GPCs fell as a function of time, concomitant with the mitotic expansion and spread of donor hGPCs. By a year after neonatal xenograft, the forebrain GPC populations of implanted mice were largely, and often entirely, of human origin. Thus, neonatally implanted hGPCs outcompeted and ultimately replaced the host population of mouse GPCs, ultimately generating mice with a humanized glial progenitor population. These human glial chimeric mice should permit us to define the specific contributions of glia to a broad variety of neurological disorders, using human cellsin vivo.
Mesoscale Transcranial Spontaneous Activity Mapping in GCaMP3 Transgenic Mice Reveals Extensive Reciprocal Connections between Areas of Somatomotor Cortex
Authors: Vanni Matthieu P. , Murphy Timothy H.
Published: November 2014

Transgenic mice expressing genetically encoded activity indicators are an attractive means of mapping mesoscopic regional functional cortical connectivity given widespread stable and cell-specific expression compatible with chronic recordings. Cortical functional connectivity was evaluated using wide-field imaging in lightly anesthetizedEmx1-creXRosa26-GCaMP3mice expressing calcium sensor in cortical neurons. Challenges exist because green fluorescence signals overlap with endogenous activity-dependent autofluorescence and are affected by changes in blood volume and oxygenation. Under the conditions used for imaging and analysis (0.1–1 Hz frequency band), autofluorescence and hemodynamic effects contributed 3% and 8% of the SD of spontaneous activity-dependent GCaMP3 fluorescence when signals were recorded through intact bone. To evaluate the accuracy and sensitivity of this approach, the topology of functional connections between somatomotor cortex (primary S1 and secondary S2 somatosensory, and primary motor cortex M1) was estimated. During sequences of spontaneous activity, calcium signals recorded at each location of area S1 were correlated with activity in contralateral area S1, ipsilateral area S2, and bilateral areas M1. Reciprocal results were observed when “seed pixels” were placed in S2 and M1. Coactivation of areas implies functional connections but could also be attributed to both regions receiving common upstream drive. These apparent connections revealed during spontaneous activity coactivation by GCaMP3 were confirmed by intracortical microstimulation but were more difficult to detect using intrinsic signals from reflected red light. We anticipate GCAMP wide-field imaging will enable longitudinal studies during plasticity paradigms or after models of CNS disease, such as stroke, where the weighting within these connectivity maps may be altered.
Poly(ADP-Ribose) Polymerase-1 Causes Mitochondrial Damage and Neuron Death Mediated by Bnip3
Authors: Lu Ping , Kamboj Amit , Gibson Spencer B. , Anderson Christopher M.
Published: November 2014

Excessive pathophysiological activity of the nuclear enzyme poly(ADP-ribose) polymerase-1 (PARP1) causes neuron death in brain hypoxia/ischemia by inducing mitochondrial permeability transition and nuclear translocation of apoptosis-inducing factor (AIF). Bcl-2/adenovirus E1B 19 kDa-interacting protein (Bnip3) is a prodeath BH3-only Bcl-2 protein family member that is induced in hypoxia, and has effects on mitochondrial permeability and neuronal survival similar to those caused by PARP1 activation. We hypothesized that Bnip3 is a critical mediator of PARP1-induced mitochondrial dysfunction and neuron death. Hypoxic death of mouse cortical neuron cultures was mitigated by deletion of either PARP1 or Bnip3, indicating that both factors are involved. Direct normoxic PARP1 activation by a DNA alkylating agent enhanced Bnip3 expression, and caused Bnip3-dependent mitochondrial membrane permeability, AIF translocation, and neuron death. Hypoxia produced PARP1-dependent depletion of nicotinamide adenine dinucleotide (NAD+) and inhibition of the NAD+-dependent class III histone deactelyase (HDAC) sirtuin-1 (SIRT1). This, in turn, led to hyperacetylation and nuclear localization of the forkhead box (Fox) protein FoxO3a, followed by enhanced association of FoxO3a with the Bnip3 upstream promoter region, increased levels of Bnip3 transcript, and elevated mitochondrial Bnip3 immunoreactivity. Finally, FoxO3a silencing using a lentiviral short hairpin RNA approach significantly reduced hypoxic Bnip3 expression, mitochondrial damage, and neuron death. Together, these data illustrate a direct PARP1-mediated hypoxic signaling pathway involving NAD+depletion, SIRT1 inhibition, FoxO3a-driven Bnip3 generation, and mitochondrial AIF release.
Futsch/MAP1B mRNA Is a Translational Target of TDP-43 and Is Neuroprotective in aDrosophilaModel of Amyotrophic Lateral Sclerosis
Authors: Coyne Alyssa N. , Siddegowda Bhavani Bagevalu , Estes Patricia S. , Johannesmeyer Jeffrey , Kovalik Tina , Daniel Scott G. , Pearson Antony , Bowser Robert , Zarnescu Daniela C.
Published: November 2014

TDP-43 is an RNA-binding protein linked to amyotrophic lateral sclerosis (ALS) that is known to regulate the splicing, transport, and storage of specific mRNAs into stress granules. Although TDP-43 has been shown to interact with translation factors, its role in protein synthesis remains unclear, and noin vivotranslation targets have been reported to date. Here we provide evidence that TDP-43 associates withfutschmRNA in a complex and regulates its expression at the neuromuscular junction (NMJ) inDrosophila. In the context of TDP-43-induced proteinopathy, there is a significant reduction offutschmRNA at the NMJ compared with motor neuron cell bodies where we find higher levels of transcript compared with controls. TDP-43 also leads to a significant reduction in Futsch protein expression at the NMJ. Polysome fractionations coupled with quantitative PCR experiments indicate that TDP-43 leads to afutschmRNA shift from actively translating polysomes to nontranslating ribonuclear protein particles, suggesting that in addition to its effect on localization, TDP-43 also regulates the translation offutschmRNA. We also show thatfutschoverexpression is neuroprotective by extending life span, reducing TDP-43 aggregation, and suppressing ALS-like locomotor dysfunction as well as NMJ abnormalities linked to microtubule and synaptic stabilization. Furthermore, the localization of MAP1B, the mammalian homolog of Futsch, is altered in ALS spinal cords in a manner similar to our observations inDrosophilamotor neurons. Together, our results suggest a microtubule-dependent mechanism in motor neuron disease caused by TDP-43-dependent alterations infutschmRNA localization and translationin vivo.
Phase–Amplitude Coupling and Interlaminar Synchrony Are Correlated in Human Neocortex
Authors: McGinn Ryan J. , Valiante Taufik A.
Published: November 2014

One of the striking manifestations of neuronal population activity is that of rhythmic oscillations in the local field potential. It is thought that such oscillatory patterns, including phase-amplitude coupling (PAC) and inter-regional synchrony, may represent forms of local and long-range cortical computations, respectively. Although it has been speculated that these two oscillatory patterns are functionally related, and bind disparate cortical assemblies to one another at different timescales, there is little direct evidence to support this hypothesis. We have demonstrated recently that theta to high-gamma PAC and interlaminar phase coherence at theta frequencies can be generated in human cortical slices maintainedin vitro. Here we show that not only do such oscillatory patterns exist within human temporal neocortex, but that the strength of one is related to the strength of the other. We demonstrate that at theta frequencies, metrics of temporal synchrony between superficial and deep cortical laminae (phase-dependent power correlations, and phase coherence) are correlated to the magnitude of intralaminar PAC between theta and high-gamma. Specifically, our results suggest that interlaminar communication within human temporal neocortex and local laminar excitability are linked to one another through a dependence mediated by theta oscillations. More generally, our results provide evidence for the hypothesis that theta oscillations may coordinate inter-areal excitability in the human brain.
Dynamic Divisive Normalization Predicts Time-Varying Value Coding in Decision-Related Circuits
Authors: Louie Kenway , LoFaro Thomas , Webb Ryan , Glimcher Paul W.
Published: November 2014

Normalization is a widespread neural computation, mediating divisive gain control in sensory processing and implementing a context-dependent value code in decision-related frontal and parietal cortices. Although decision-making is a dynamic process with complex temporal characteristics, most models of normalization are time-independent and little is known about the dynamic interaction of normalization and choice. Here, we show that a simple differential equation model of normalization explains the characteristic phasic-sustained pattern of cortical decision activity and predicts specific normalization dynamics: value coding during initial transients, time-varying value modulation, and delayed onset of contextual information. Empirically, we observe these predicted dynamics in saccade-related neurons in monkey lateral intraparietal cortex. Furthermore, such models naturally incorporate a time-weighted average of past activity, implementing an intrinsic reference-dependence in value coding. These results suggest that a single network mechanism can explain both transient and sustained decision activity, emphasizing the importance of a dynamic view of normalization in neural coding.
Attentional Modulation of Alpha/Beta and Gamma Oscillations Reflect Functionally Distinct Processes
Authors: Bauer Markus , Stenner Max-Philipp , Friston Karl J. , Dolan Raymond J.
Published: November 2014

The brain adapts to dynamic environments by adjusting the attentional gain or precision afforded to salient and predictable sensory input. Previous research suggests that this involves the regulation of cortical excitability (reflected in prestimulus alpha oscillations) before stimulus onset that modulates subsequent stimulus processing (reflected in stimulus-bound gamma oscillations). We present two spatial attention experiments in humans, where we first replicate the classic finding of prestimulus attentional alpha modulation and poststimulus gamma modulation. In the second experiment, the task-relevant target was a stimulus change that occurred after stimulus onset. This enabled us to show that attentional alpha modulation reflects the predictability (precision) of an upcoming sensory target, rather than an attenuation of alpha activity induced by neuronal excitation related to stimulus onset. In particular, we show that the strength of attentional alpha modulations increases with the predictability of the anticipated sensory target, regardless of current afferent drive. By contrast, we show that the poststimulus attentional gamma enhancement is stimulus-bound and decreases when the subsequent target becomes more predictable. Hence, this pattern suggests that the strength of gamma oscillations is not merely a function of cortical excitability, but also depends on the relative mismatch of predictions and sensory evidence. Together, these findings support recent theoretical proposals for distinct roles of alpha/beta and gamma oscillations in hierarchical perceptual inference and predictive coding.
Neuronal Regeneration inC. elegansRequires Subcellular Calcium Release by Ryanodine Receptor Channels and Can Be Enhanced by Optogenetic Stimulation
Authors: Sun Lin , Shay James , McLoed Melissa , Roodhouse Kevin , Chung Samuel H. , Clark Christopher M. , Pirri Jennifer K. , Alkema Mark J. , Gabel Christopher V.
Published: November 2014

Regulated calcium signals play conserved instructive roles in neuronal repair, but how localized calcium stores are differentially mobilized, or might be directly manipulated, to stimulate regeneration within native contexts is poorly understood. We find here that localized calcium release from the endoplasmic reticulum via ryanodine receptor (RyR) channels is critical in stimulating initial regeneration following traumatic cellular damagein vivo. Using laser axotomy of single neurons inCaenorhabditis elegans, we find that mutation ofunc-68/RyR greatly impedes both outgrowth and guidance of the regenerating neuron. Performing extendedin vivocalcium imaging, we measure subcellular calcium signals within the immediate vicinity of the regenerating axon end that are sustained for hours following axotomy and completely eliminated withinunc-68/RyR mutants. Finally, using a novel optogenetic approach to periodically photo-stimulate the axotomized neuron, we can enhance its regeneration. The enhanced outgrowth depends on both amplitude and temporal pattern of excitation and can be blocked by disruption of UNC-68/RyR. This demonstrates the exciting potential of emerging optogenetic technology to beneficially manipulate cell physiology in the context of neuronal regeneration and indicates a link to the underlying cellular calcium signal. Taken as a whole, our findings define a specific localized calcium signal mediated by RyR channel activity that stimulates regenerative outgrowth, which may be dynamically manipulated for beneficial neurotherapeutic effects.
Single-Trial Properties of Place Cells in Control and CA1 NMDA Receptor Subunit 1-KO Mice
Authors: Cabral Henrique O. , Fouquet Celine , Rondi-Reig Laure , Pennartz Cyriel M.A. , Battaglia Francesco P.
Published: November 2014

The NMDA receptor plays a key role in synaptic plasticity and its disruption leads to impaired spatial representation in the CA1 area of the hippocampus, with place cells exhibiting larger place fields (McHugh et al., 1996). Place fields are defined by the spatial and nonspatial inputs of a given place and context, by intrinsic network processes, such as phase precession, but also by the matching of these inputs to a pre-existing spatial representation. Larger place fields may be a consequence of spatially widened firing upon a single crossing of a place field, or of increased variability in place field positions across traversals. We addressed this question by monitoring CA1 place cell activity, with tetrodes, in control and KO mice lacking the NMDA receptor in this region. In individual crossings of the field, we found no difference between genotypes in place field size; the larger, overall place field size turns out to be a consequence of jitter across trials. We suggest that this jitter reflects a deficit in the matching of current spatial inputs to the stored spatial representation of the track. This is supported by the finding that deficits in place field size and spatial information are rescued by extensive exposure of the mouse to the track, which may echo an increased influence of memory retrieval processes in CA3 on firing in CA1.
A new prozostrodontian cynodont (Therapsida) from the Late Triassic Riograndia Assemblage Zone (Santa Maria Supersequence) of Southern Brazil
Authors: SOARES MARINA B. , MARTINELLI AGUSTÍN G. , OLIVEIRA TÉO V. DE
Published: November 2014

We report here on a new prozostrodontian cynodont, Botucaraitherium belarminoi gen. et sp. nov., from the Late Triassic Riograndia Assemblage Zone (AZ) of the Candelária Sequence (Santa Maria Supersequence), collected in the Botucaraí Hill Site, Candelária Municipality, state of Rio Grande do Sul, Brazil. The new taxon is based on a single specimen (holotype MMACR-PV-003-T) which includes the left lower jaw, without postdentary bones, bearing the root of the last incisor, canine and four postcanines plus one partial crown inside the dentary, not erupted, and two maxillary fragments, one with a broken canine and another with one postcanine. The features of the lower jaw and lower/upper postcanines resemble those of the prozostrodontians Prozostrodon brasiliensis from the older Hyperodapedon AZ and Brasilodon quadrangularis and Brasilitherium riograndensis from the same Riograndia AZ. The inclusion of Botucaraitherium within a broad phylogenetic analysis, positioned it as a more derived taxon than tritylodontids, being the sister-taxon of Brasilodon, Brasilitherium plus Mammaliaformes. Although the new taxon is based on few cranial elements, it represents a additional faunal component of the Triassic Riograndia AZ of southern Brazil, in which small-sized derived non-mammaliaform cynodonts, closely related to the origin of mammaliaforms, were ecologically well succeed and taxonomically diverse.
A aplicação da distribuição exponencial geométrica estendida para modelagem de dados pluviométricos
Authors: Ramos Pedro Luiz , Moala Fernando Antonio
Published: November 2014

Neste trabalho propõem-se o uso da distribuição Exponencial Geométrica Estendida (EGE) como um modelo alternativo às distribuições comumente utilizadas tais como Gama, Weibull, Lognormal, entre outras, para a modelagem de dados de precipitação pluvial. Pouco explorada na literatura, a distribuição EGE tem se mostrado eficiente em diversos campos de pesquisa como biologia, demografia, confiabilidade de produtos eletrônicos e pode ser aplicada para analisar fenômenos meteorológicos. Proposta por Adamidis e colaboradores em 2005, uma de suas particularidades é que sua função de risco pode ser crescente ou decrescente. Outra característica importante é a facilidade em se obter diferentes níveis de probabilidade, sem a necessidade de recorrer a métodos numéricos. Testou-se o ajustamento da distribuição EGE para a estimação da precipitação pluvial total mensal de Presidente Prudente-SP. Os resultados mostraram que houve um bom ajuste do modelo para os dados ao serem comparados com outros modelos como Gama, Weibull e Lognormal, de acordo com o critério de informação de Akaike, o teste Kolmogorov-Smirnov e o teste Qui-quadrado ao nivel de 5% de significância. A partir do ajustamento da distribuição EGE aos dados, os estimadores dos parâmetros da distribuição foram obtidos através do método de máxima verossimilhança permitindo assim a estimação da precipitação pluvial total mensal para diferentes níveis de probabilidade.
Características de crescimento, composição química, física e estimativa de massa seca de madeira em clones e espécies de Eucalyptus jovens
Authors: Trugilho Paulo Fernando , Goulart Selma Lopes , Assis Claudinéia Olímpia de , Couto Fernanda Bueno Sarro , Alves Isabel Cristina Nogueira , Protásio Thiago de Paula , Napoli Alfredo
Published: November 2014

O objetivo deste trabalho foi avaliar o efeito do material genético, clones e espécies, e da classe de diâmetro nas características de crescimento, composição química, densidade básica e a estimativa de massa seca de madeira. Foram avaliados quatro clones e cinco espécies, aos quatro anos de idade, de Eucalyptus procedentes da empresa RIMA Industrial S.A. Foram avaliadas cinco árvores por clone e espécie em três e quatro classes diamétricas, respectivamente. Avaliaram-se as características individuais de crescimento da árvore, a densidade básica, a estimativa de massa seca, o teor de lignina, extrativos totais, holocelulose e cinzas. O volume, com e sem casca, e a estimativa de massa seca apresentaram efeito significativo de material genético (clone e espécie) e classe diamétrica. O teor de lignina não foi influenciado significativamente pelo material genético e classe diamétrica. A densidade básica apresentou efeito significativo de classe diamétrica para os clones e de material genético para as espécies. O teste de identidade de modelos somente foi não significativo para a estimativa de massa seca para as espécies, possibilitando usar a equação comum independente da espécie. Os clones apresentaram madeira mais homogênea, com menor densidade básica, extrativos totais e lignina total e maior teor de holocelulose, características mais adequadas à produção de polpa celulósica. As espécies apresentaram madeira mais heterogênea, com maior densidade básica, estimativa de massa seca, extrativos totais e lignina total e menor teor de cinzas e holocelulose, características desejáveis à produção de bioenergia
Development of psychiatric risk evaluation checklist and routine for nurses in a general hospital: ethnographic qualitative study
Authors: Camargo Ana Luiza Lourenço Simões , Maluf Neto Alfredo , Colman Fátima Tahira , Citero Vanessa de Albuquerque
Published: November 2014

CONTEXT AND OBJECTIVE:There is high prevalence of mental and behavioral disorders in general hospitals, thus triggering psychiatric risk situations. This study aimed to develop a psychiatric risk assessment checklist and routine for nurses, the Psychiatric Risk Evaluation Check-List (PRE-CL), as an alternative model for early identification and management of these situations in general hospitals.DESIGN AND SETTING:Ethnographic qualitative study in a tertiary-level private hospital.METHOD:Three hundred general-unit nurses participated in the study. Reports were gathered through open groups conducted by a trained nurse, at shift changes for two months. The questions used were: "Would you consider it helpful to discuss daily practice situations with a psychiatrist? Which situations?" The data were qualitatively analyzed through an ethnographic approach.RESULTS:The nurses considered it useful to discuss daily practice situations relating to mental and behavioral disorders with a psychiatrist. Their reports were used to develop PRE-CL, within the patient overall risk assessment routine for all inpatients within 24 hours after admission and every 48 hours thereafter. Whenever one item was present, the psychosomatic medicine team was notified. They went to the unit, gathered data from the nurses, patient files and, if necessary, attending doctors, and decided on the risk management: guidance, safety measures or mental health consultation.CONCLUSION:It is possible to develop a model for detecting and intervening in psychiatric and behavioral disorders at general hospitals based on nursing team observations, through a checklist that takes these observations into account and a routine inserted into daily practice.
Morphological effects of induced polyploidy in Dendrobium nobile Lindl. (Orchidaceae)
Authors: Vichiato Mívia Rosa de Medeiros , Vichiato Marcelo , Pasqual Moacir , Rodrigues Filipe Almendagna , Castro Daniel Melo de
Published: November 2014

In general, polyploidy in plants causes an increase in the size ofvegetative structures. This work aimed to compare diploid plants (2n = 2 x =38) and induced tetraploid plants (2n = 4 x = 76) of Dendrobium nobile Lindl. through the evaluation of themorphological characteristics of flowers, leaves and pseudobulbs. We evaluatedthe parameters height and diameter of pseudobulbs; width and length of leavesand flowers (sepals, petals and labella); and diameter of flowers. The inducedpolyploidization resulted in the increasing the number of internodes (19.9%)and floral pieces, with greater height of the flower (4.5%) and width of thelip (18.5%) decrease in the number of flowers per pseudobulb (40.9%) and thediameter of the pseudobulb (64.9%) and delayed flowering.
Implications of selection in common bean lines in contrasting environments concerning nitrogen levels
Authors: Furtini Isabela Volpi , Ramalho Magno Antonio Patto , Abreu Ângela de Fátima Barbosa
Published: November 2014

Grain productivities of 100 bean lines were evaluated in the presence and absence of nitrogen fertilizer in order to identify those with high nitrogen use efficiency (NUE) and to determine the correlated response observed in a stressed environment following selection in a non-stressed environment. The genetic and phenotypic characteristics of the lines, as wellas the response index to applied nitrogen, were determined. The average grain productivities at both locations were 39.5% higher in the presence of nitrogen fertilizer, with 8.3 kg of grain being produced per kg of nitrogen applied. NUE varied greatly between lines. Lines BP-16, CVII-85-11, BP-24, Ouro Negro and MA-IV-15-203 were the most efficient and responsive. The results showed that it is possible to select bean lines in stressed and non-stressed environments. It was inferred that common bean lines for environments with low nitrogenav ailability should preferably be selected under nitrogen stress.
Análise bayesiana univariada e bivariada para a conversão alimentar de suínos da raça Piau
Authors: Rossi Robson Marcelo , Martins Elias Nunes , Lopes Paulo Sávio , Silva Fabyano Fonseca e
Published: November 2014

O objetivo deste trabalho foi apresentar modelagens alternativas, uni e bivariadas, para avaliação da conversão alimentar (CA) de suínos da raça Piau, com uso de inferência bayesiana. Os efeitos de sexo e genótipo sobre a CA dos animais foram avaliados por meio de procedimentos de simulação de Monte Carlo via cadeias de Markov (MCMC) e de integração aproximada aninhada de Laplace (INLA). O modelo univariado foi avaliado com diferentes distribuições para o erro - normal (gaussiana), t de Student, gama, log-normal e skew-normal -, enquanto, para o modelo bivariado, considerou-se o erro normal. A distribuição skew-normal foi o modelo mais parcimonioso para inferir sobre a resposta direta (univariada) da CA aos efeitos de sexo e genótipo, os quais não foram significativos. O modelo bivariado foi capaz de identificar diferenças significativas no ganho de peso e no consumo de ração em níveis de significância não detectados pelo modelo univariado. Além disso, ele também foi capaz de detectar diferenças entre sexos, quando agrupados por genótipos NN (machos, 2,73±0,04; fêmeas, 2,68±0,04) e Nn (machos, 2,70±0,07; fêmeas, 2,64±0,07), e revelou maior acurácia e precisão nas inferências nutricionais. Em ambas as abordagens, o método bayesiano mostra-se flexível e eficiente para a avaliação do desempenho nutricional dos animais.
Inheritance of late flowering in natural variants of soybean cultivars under short-day conditions
Authors: Carpentieri-Pipolo Valeria , Almeida Leones Alves de , Kiihl Romeu Afonso de Souza , Pagliosa Eduardo Stefani
Published: November 2014

The objective of this work was to determine the inheritance of the long juvenile period trait in natural variants of the Doko, BR 9 (Savana), Davis, Embrapa 1 (IAS 5RC), and BR 16 soybean cultivars. Complete diallel crosses were made between the Doko and BR 16 cultivars and their variants. A 3:1 segregation ratio was observed in the F2 populations of the 'Doko' x Doko-18T, 'Doko' x Doko-Milionária, 'Davis' x São Carlos, and 'BR 9 (Savana)' x MABR92-836 (Savanão) crosses, indicating that the long juvenile period trait is controlled by a pair of recessive genes. The difference in late flowering between the Doko cultivar and both of its variants was caused by a recessive spontaneous mutation at the same genetic locus. However, the variants Doko-18T and Doko-Milionária are identical mutants that share a pair of genes that control the long juvenile period under short-day conditions. These mutants can be used in breeding programs to develop cultivars adapted to low-latitude tropical regions.
Hormonal protocols for in vitro production of Zebu and taurine embryos
Authors: Fernandes Carlos Antônio de Carvalho , Miyauchi Tatimara Maria , Figueiredo Ana Cristina Silva de , Palhão Miller Pereira , Varago Fabiana Cristina , Nogueira Ester Siqueira Caixeta , Neves Jairo Pereira , Miyauchi Tochimara Aparecida
Published: November 2014

The objective of this work was to evaluate the effects of hormonal synchronization protocols, associated or not with follicular development stimulation, on the recovery of oocytes and on in vitro production of Bos indicus and B. taurus embryos, in different seasons. Ultrasound-guided follicular aspirations (n=237) were performed without pre-treatment (G1, control group) and after follicular wave synchronization (G2), or after follicular wave synchronization and follicle growth induction (G3). Bos indicus produced more oocytes and embryos than B. taurus (18.7±0.9 vs. 11.9±0.6 oocytes and 4.8±0.3 vs. 2.1±0.2 embryos). On average, oocyte and embryo yields were higher in G3 than in G2, and both were greater than in G1, which lead to a higher conversion of oocytes to embryos in these treatments. The hot or the cold season did not affect the B. indicus outcomes, whereas, in B. taurus, both oocyte recovery and embryo production were higher in the cold season. Follicular wave synchronization improves ovum pick-up and in vitro production of embryos in both cattle subspecies evaluated.
A adição de xisto retortado aumenta a retenção do carbono de resíduos vegetais no solo
Authors: Leão Ricardo Elso , Giacomini Sandro José , Redin Marciel , Souza Eduardo Lorensi , Silveira Carlos Augusto Posser
Published: November 2014

O objetivo deste trabalho foi avaliar o efeito de curto prazo e o residual de doses de xisto retortado (XR) sobre a retenção do C de resíduos culturais no solo. Foram avaliadas a mineralização e a retenção de C de folhas e talos de soja enriquecidos com 13C, em solo com e sem histórico de aplicação de XR e na presença e na ausência de doses crescentes de XR. Houve efeito de curto prazo do XR sobre a retenção de C no solo. Esse efeito ocorreu somente com a mistura de folhas + 3 Mg ha-1 de XR, em que a retenção de C no solo superou em 21% aquela observada com a aplicação isolada das folhas. O XR apresenta potencial de reter C no solo.
Patotipos de Escherichia coli causadores de diarreia em bezerros: uma atualização
Authors: Coura Fernanda M. , Lage Andrey P. , Heinemann Marcos B.
Published: November 2014

A diarreia é uma das doenças mais frequentes de bezerros com até 30 dias de idade e é uma importante causa de perdas econômicas. Sua etiologia é complexa e envolve a interação de diversos fatores infecciosos, nutricionais, imunológicos, gerenciais e ambientais. Os principais sinais clínicos são a diarreia, desidratação progressiva, acidose metabólica, desequilíbrio de eletrólitos e balanço energético negativo com ou sem hipoglicemia, que se não tratados, levam à morte do animal. Escherichia coli se destaca como um importante enteropatógeno envolvido na síndrome diarreica. Cepas de E. coli patogênicas são classificadas em grupos ou patotipos, de acordo com a produção de fatores de virulência e mecanismos pelos quais causam doença. Já foram identificados cinco patotipos de E. coli associados à diarreia em bezerros: E. coli enterotoxigênica (ETEC), E. coli enteropatogênica (EPEC), E. coli enterohemorrágica (EHEC), E. coli produtora de toxina Shiga (STEC) e E. coli necrotoxigênica (NTEC). Nesse artigo apresentamos as principais características e os atuais conhecimentos sobre os patotipos de E. coli causadores de diarreia em bezerros.
Aspectos epidemiológicos, clínicos e anatomopatológicos do linfoma em bovinos: 128 casos (1965-2013)
Authors: Panziera Welden , Bianchi Ronaldo M. , Galiza Glauco J.N. , Pereira Paula R. , Mazaro Renata D. , Barros Claudio S.L. , Kommers Glaucia D. , Irigoyen Luiz F. , Fighera Rafael A.
Published: November 2014

Por meio de um estudo retrospectivo, os aspectos epidemiológicos, clínicos e anatomopatológicos de 128 casos de linfoma bovino são descritos. Dos protocolos que informavam o sexo (n=111), 84,7% correspondiam a fêmeas e 15,3% a machos. Dos protocolos em que constava a raça (n=108), a mais prevalente foi a holandesa (63%). Em relação à idade (n=107), houve uma variação entre um e 14 anos. A maioria dos bovinos era adulta (89,7%) e a maior concentração dos casos ocorreu ao redor de 5-8 anos (57,9%). Em relação aos sinais clínicos (n=89), linfadenomegalia foi o achado mais frequentemente observado (74,1%). Outros sinais clínicos, principalmente aqueles relacionados com os sistemas respiratório (dispneia, estertoração pulmonar e taquipneia), cardiovascular (taquicardia, edema subcutâneo e pulso venoso positivo), digestório (atonia ruminal, timpanismo e diarreia) e nervoso (paresia dos membros pélvicos e andar cambaleante), foram pouco prevalentes. Na necropsia (n=125), 71,2% dos bovinos apresentavam aumento de volume dos linfonodos; essa linfadenomegalia foi classificada como localizada em 89,6% dos casos e generalizada em 10,3% dos casos. Dos protocolos que informavam os linfonodos acometidos (n=58), a distribuição foi a seguinte: mesentéricos (51,7%), mediastínicos (37,9%), pré-escapulares (29,3%), ilíacos internos (27,6%), inguinais superficiais (25,8%) e traqueobrônquicos (18,9%). Além dos linfonodos, outros órgãos comumente afetados pelo linfoma neste estudo incluíram: coração (40%), fígado (15,2%), rim (14,4%), abomaso (12,8%), útero (11,2%), intestino (10,4%) e pulmão (7,2%). A presença de massas tumorais no canal vertebral foi observada em poucos casos (3,2%). Com base na epidemiologia e na localização das lesões, a maioria dos casos (96%) foi classificada como linfoma enzoótico e o restante (4%) como linfoma esporádico. Os resultados encontrados neste estudo irão auxiliar clínicos de grandes animais e patologistas veterinários na suspeita e no diagnóstico definitivo do linfoma na espécie bovina.
Aspectos histopatológicos das lesões renais em gatos experimentalmente infectados pelo vírus da imunodeficiência felina
Authors: Mesquita Leonardo P. , Haibara Denise , Zanutto Marcelo S. , Sá Lilian R.M. de , Hagiwara Mitika K. , Maiorka Paulo C.
Published: November 2014

A infecção pelo vírus da imunodeficiência felina (FIV) em gatos domésticos é caracterizada por distúrbios imunológicos, que geralmente se manifestam tardiamente na doença. Semelhante à infecção pelo vírus da imunodeficiência humana (HIV) em humanos, a infecção pelo FIV geralmente está associada a infecções oportunistas e ao desenvolvimento progressivo de nefropatia. Portanto, o objetivo do presente estudo foi avaliar as alterações histopatológicas em rins de 10 gatos experimentalmente infectados pelo FIV submetidos a eutanásia 60 meses após a inoculação viral. Nos rins de 100% dos gatos infectados pelo FIV foram visualizadas lesões glomerulares e tubulointersticiais. As lesões glomerulares eram caracterizadas principalmente por espessamento global ou segmentar da membrana basal glomerular (glomerulonefrite membranosa). Glomeruloesclerose e, em dois casos, proliferação de células epiteliais intraglomerulares (crescente glomerular), também foram observados. Nefrite intersticial linfoplasmocítica foi a alteração tubulointersticial mais frequente, visualizada em diferentes intensidades nos rins de 100% dos gatos. Os resultados do presente estudo demonstram que o tempo prolongado entre a infecção e a avaliação histopatológica pode ter sido decisivo para o surgimento das lesões renais em todos os gatos infectados pelo FIV e para o agravamento dessas lesões em alguns gatos.
Diagnóstico imuno-histoquímico e caracterização anatomopatológica de clamidiose em psitacídeos
Authors: Casagrande Renata A. , Machado Veronica R. , Souza Suyene O. de , Watanabe Tatiane T.N. , Sonne Luciana , Pavarini Saulo P. , Driemeier David
Published: November 2014

A clamidiose é causada por Chlamydophila psittaci e representa uma das principais zoonoses de origem aviária. Realizou-se um estudo retrospectivo em psitacídeos do período de 1995 a 2012 e exame imuno-histoquímico (IHQ) anti-Chlamydia. Foram avaliados 111 casos, dos quais 12 foram a óbito devido à clamidiose. As aves eram provenientes de apreensão ou cativeiro (zoológicos, criatórios, centros de triagem e domicílios). À necropsia observou-se fígado aumentado (4/12) com áreas branco-amareladas (3/12), baço aumentado (2/12) e rompido (1/12), saco pericárdico com deposição de fibrina (1/12), polisserosite fibrinosa (1/12) e em três casos não havia lesões. Na avaliação histopatológica evidenciou-se hepatite necrótica mononuclear (7/12), hepatite mononuclear (3/12), hiperplasia de ductos biliares (8/12), esplenite necrótica histiocitária (9/12), hemossiderose em fígado (9/12) e baço (9/12), aerossaculite mononuclear (4/12), pericardite fibrino-heterofílica (2/12), necrose (1/12) e rarefação (1/12) linfoide de bursa de Fabricius, pneumonia fibrinosa (1/12), nefrite mononuclear (1/12) e granulomas renais (1/12). Observaram-se inclusões basofílicas intracitoplasmáticas (corpos elementares) em fígado (2/12), baço e rins (1/12). Evidenciou-se imunomarcação anti-Chlamydia em fígado (11/12), baço (7/9), pulmões (3/9), rins (2/8), intestinos (2/3), sacos aéreos (1/4) e bursa de Fabricius (1/2). A IHQ poderá ser utilizada como forma de diagnóstico definitivo post mortem de clamidiose em psitacídeos no Brasil.