bluuebunny/crossref_metadata_2025 huggingface dataset:
CO2 Capture from Flue Gas of a Coal-Fired Power Plant Using Three-Bed PSA Process
Authors: Cheng Chu-Yun , Kuo Chia-Chen , Yang Ming-Wei , Zhuang Zong-Yu , Lin Po-Wei , Chen Yi-Fang , Yang Hong-Sung , Chou Cheng-Tung
Published: June 2021

The pressure swing adsorption (PSA) process was used to capture carbon dioxide (CO2) from the flue gas of a coal-fired power plant to reduce CO2 emissions. Herein, CO2 was captured from flue gas using the PSA process for at least 85 vol% CO2 purity and with the other exit stream from the process of more than 90 vol% N2 purity. The extended Langmuir–Freundlich isotherm was used for calculating the equilibrium adsorption capacity, and the linear driving force model was used to describe the gas adsorption kinetics. We compared the results of breakthrough curves obtained through experiments and simulations to verify the accuracy of the mass transfer coefficient. The flue gas obtained after desulphurization and water removal (13.5 vol% CO2 and 86.5 vol% N2) from a subcritical 1-kW coal-fired power plant served as the feed for the designed three-bed, nine-step PSA process. To determine optimal operating conditions for the process, the central composite design (CCD) was used. After CCD analysis, optimal operating conditions with a feed pressure of 3.66 atm and a vacuum pressure of 0.05 atm were obtained to produce a bottom product with a CO2 purity of 89.20 vol% and a recovery of 88.20%, and a top product with a N2 purity of 98.49 vol% and a recovery of 93.56%. The mechanical energy consumption was estimated to be 1.17 GJ/t-CO2.
Innovation in Start-Up Business Model in Energy-Saving Solutions for Sustainable Development
Authors: Wit Bogdan , Dresler Piotr , Surma-Syta Anna
Published: June 2021

Socially expected innovations are innovations considering sustainable development. The subject of the paper focuses on the business model of a start-up providing energy saving services to local government units using smart technologies of Industry 4.0 in the aspect of low touch economy. A methodical critical literature review including quantitative and qualitative assessment, stakeholder analysis and business modeling techniques using Business Model Canvas and Triple Layer Business Model Canvas (TLBMC) was conducted. In addition, an in-depth analysis of a start-up case study was conducted. The research questions are related to the interpretation of the organization’s business data and methods of interpreting Sustainability 3.0 business solutions. The research questions were directed to the challenges regarding the creation of the organization’s sustainable business model architecture and the Business Sustainability 3.0 sustainable business imaging concept. The research objective is to design a sustainable business model of a start-up providing energy-efficient services to local government units, whose value proposition refers to an extended sustainable value that meets the economic, social and environmental needs of society. The integration of sustainability in the sustainable business model of the start-up allowed to achieve the research objective of designing a sustainable value proposition that meets the economic, social and environmental needs of society.
Cascaded Smart Gate Drivers for Modular Multilevel Converters Control: A Decentralized Voltage Balancing Algorithm
Authors: Darbas Corentin , Olivier Jean-Christophe , Ginot Nicolas , Poitiers Frédéric , Batard Christophe
Published: June 2021

Recent Modular Multilevel Converter (MMC) topology allows for drastic improvements in power electronic conversion such as higher energy quality, lower power semiconductors electrical stress, decreased Electro-Magnetic Interferences (EMI), and reduced switching losses. MMC is widely used in High Voltage Direct-Current (HVDC) transmissions as it offers, theoretically, no voltage limit. However, its control electronic structure is not modular itself. Especially, the insulation voltage between the submodule gate drivers’ primaries and secondaries depends on the number of submodules. The converter voltage levels cannot be increased without designing all gate driver isolations again. To solve that issue, the novel concept of distributed galvanic insulation is introduced for multilevel converters. The submodule’s gate drivers are daisy-chained, which naturally reduces the insulation voltage to the submodule capacitor voltage, regardless of the number of submodules. The MMC becomes truly modular as the number of submodules can be increased without impacting on the previous control electronic circuit. Such an innovative control structure weakens the link between the main control unit and the gate drivers. This inherent structural problem can be solved through the use of Smart-Gate Drivers (SGD), as they are often equipped with fast and bidirectional communication channels, while highly increasing the converter reliability. The innovation proposed in that work is the involvement of smart gate drivers in the distributed galvanic insulation-based MMC control and monitoring. First, the numerous benefits of smart gate drivers are discussed. Then, an innovative Voltage Balancing Algorithm directly integrated on the chained gate drivers is proposed and detailed. It features a tunable parameter, offering a trade-off between accurate voltage balancing and execution time. The proposed embedded algorithm features a low execution time due to simultaneous voltage comparisons. Such an algorithm is executed by the gate drivers themselves, relieving the main control unit in an original decentralized control scheme. A simulation model of a multi-megawatts three-phase grid-tied MMC inverter is realized, allowing validation of the proposed algorithm. Matlab/Simulink logic blocs allow us to simulate a typical CPLD/FPGA component, often embedded on smart gate drivers. The converter with the proposed embedded algorithm is simulated in steady-state and during load impact. The controlled delay and slew rate inferred by the algorithm do not disturb the converter behavior, allowing its conceptual validation.
Transcriptomic and Anatomic Profiling Reveal Etiolation Promotes Adventitious Rooting by Exogenous Application of 1-Naphthalene Acetic Acid in Robinia pseudoacacia L.
Authors: Munir Muhammad Zeeshan , Ud Din Saleem , Imran Muhammad , Zhang Zijie , Pervaiz Tariq , Han Chao , Un Nisa Zaib , Bakhsh Ali , Atif Muneer Muhammad , Sun Yuhan , Li Yun
Published: June 2021

The process of etiolation contributes significantly to vegetative propagation and root formation of woody plants. However, the molecular interaction pattern of different factors for etiolated adventitious root development in woody plants remains unclear. In the present study, we explored the changes at different etiolation stages of adventitious root formation in Robinia pseudoacacia. Histological and transcriptomic analyses were performed for the etiolated lower portion of hypocotyls to ascertain the adventitious root responses. We found that the dark-treated hypocotyls formed roots earlier than the control. Exogenous application of NAA (0.3 mg/L) stimulated the expressions of about 310 genes. Among these, 155 were upregulated and 155 were downregulated. Moreover, differentially expressed genes (DEGs) were significantly enriched in multiple pathways, including the biosynthesis of secondary metabolites, metabolic pathway, plant hormone signal transduction, starch and sucrose metabolism, phenylpropanoid biosynthesis, and carbon metabolism. These pathways could play a significant role during adventitious root formation in etiolated hypocotyls. The findings of this study can provide novel insights and a foundation for further studies to elucidate the connection between etiolation and adventitious root formation in woody plants.
Full-Length Transcriptome-Wide Characteristic and Functional Identification of WRKY Family in Malus sieversii during the Valsa Canker Disease Response
Authors: Liu Xiaojie , Zhang Yiheng , Zhou Tong , Li Xiaoshuang , Wen Xuejing , Zhang Daoyuan
Published: June 2021

WRKY transcription factors are one of the largest families in plants, playing important roles in regulating plant immunity. Malus sievesii has abundant genetic diversity and can offer various and high-quality gene resources. In this study, 112 putative MsWRKY proteins were identified from a full-length transcriptome of M. sieversii during the Valsa canker disease (caused by Valsa mali). The MsWRKY proteins were phylogenetically divided into three groups (I–III). Motif compositions of the MsWRKY proteins were clustered and fifteen conserved motifs were observed. Expression pattern analysis showed that thirty-four MsWRKY transcripts strongly responded to the V. mali infection, demonstrating that MsWRKY transcripts might play different roles during the response. Functional identifications were subsequently conducted with transient expressions, demonstrating that MsWRKY16, MsWRKY21, MsWRKY70, MsWRKY74 and MsWRKY85 positively regulated the resistant response. Besides, the MsWRKY21, MsWRKY70 and MsWRKY85 were dramatically induced by salicylic acid (SA), methyl-jasmonate acid (MeJA) and 1-aminocyclopropane-1-carboxylate (ACC), indicating that they play important roles in the regulatory resistance of V. mali infection. This work provides a comprehensive understanding of the WRKY family in M. sieversii and will build a foundation for future research of the potential disease resistances MsWRKY transcripts.
Silvicultural Interventions Drive the Changes in Soil Organic Carbon in Romanian Forests According to Two Model Simulations
Authors: Blujdea Viorel N. B. , Viskari Toni , Kulmala Liisa , Gârbacea George , Dutcă Ioan , Miclăuș Mihaela , Marin Gheorghe , Liski Jari
Published: June 2021

We investigated the effects of forest management on the carbon (C) dynamics in Romanian forest soils, using two model simulations: CBM-CFS3 and Yasso15. Default parametrization of the models and harmonized litterfall simulated by CBM provided satisfactory results when compared to observed data from National Forest Inventory (NFI). We explored a stratification approach to investigate the improvement of soil C prediction. For stratification on forest types only, the NRMSE (i.e., normalized RMSE of simulated vs. NFI) was approximately 26%, for both models; the NRMSE values reduced to 13% when stratification was done based on climate only. Assuming the continuation of the current forest management practices for a period of 50 years, both models simulated a very small C sink during simulation period (0.05 MgC ha−1 yr−1). Yet, a change towards extensive forest management practices would yield a constant, minor accumulation of soil C, while more intensive practices would yield a constant, minor loss of soil C. For the maximum wood supply scenario (entire volume increment is removed by silvicultural interventions during the simulated period) Yasso15 resulted in larger emissions (−0.3 MgC ha−1 yr−1) than CBM (−0.1 MgC ha−1 yr−1). Under ‘no interventions’ scenario, both models simulated a stable accumulation of C which was, nevertheless, larger in Yasso15 (0.35 MgC ha−1 yr−1) compared to CBM-CSF (0.18 MgC ha−1 yr−1). The simulation of C stock change showed a strong “start-up” effect during the first decade of the simulation, for both models, explained by the difference in litterfall applied to each scenario compared to the spinoff scenario. Stratification at regional scale based on climate and forest types, represented a reasonable spatial stratification, that improved the prediction of soil C stock and stock change.
Whole Exome Sequencing of 23 Multigeneration Idiopathic Scoliosis Families Reveals Enrichments in Cytoskeletal Variants, Suggests Highly Polygenic Disease
Authors: Terhune Elizabeth A. , Wethey Cambria I. , Cuevas Melissa T. , Monley Anna M. , Baschal Erin E. , Bland Morgan R. , Baschal Robin , Trahan G. Devon , Taylor Matthew R. G. , Jones Kenneth L. , Hadley Miller Nancy
Published: June 2021

Adolescent idiopathic scoliosis (AIS) is a lateral spinal curvature >10° with rotation that affects 2–3% of healthy children across populations. AIS is known to have a significant genetic component, and despite a handful of risk loci identified in unrelated individuals by GWAS and next-generation sequencing methods, the underlying etiology of the condition remains largely unknown. In this study, we performed exome sequencing of affected individuals within 23 multigenerational families, with the hypothesis that the occurrence of rare, low frequency, disease-causing variants will co-occur in distantly related, affected individuals. Bioinformatic filtering of uncommon, potentially damaging variants shared by all sequenced family members revealed 1448 variants in 1160 genes across the 23 families, with 132 genes shared by two or more families. Ten genes were shared by >4 families, and no genes were shared by all. Gene enrichment analysis showed an enrichment of variants in cytoskeletal and extracellular matrix related processes. These data support a model that AIS is a highly polygenic disease, with few variant-containing genes shared between affected individuals across different family lineages. This work presents a novel resource for further exploration in familial AIS genetic research.
Forgotten Contributors in the Brick Sector in Nepal
Authors: Bajracharya Sugat B. , Gurung Kamala , Mathema Luja , Sharma Sanjay , Mishra Arabinda
Published: June 2021

Brick making in Nepal is an informal sector that is still highly labor intensive. It employs transient workers who are extremely marginalized and exposed to poor working and living conditions. This study assesses the working and living conditions of male and female brick workers and their children and looks to address the main issues and challenges to promote decent living and working conditions in the brick factories through action research. A rapid needs assessment was conducted to take stock of the working and living conditions of male and female workers across five provinces in Nepal. Subsequently, selected pilot interventions through stakeholder consultations were initiated to address these issues and challenges. There are a host of challenges faced by these workers in the factories, with the main issues being occupational health and safety and childcare/education for workers’ children. The study suggests that the working and living conditions of the workers can be improved by incentivizing the brick entrepreneurs to invest in them, thus creating a more productive workforce. Moreover, the findings from the pilot interventions can help in the design of effective solutions.
“In a Way We Took the Hospital Home”—A Descriptive Mixed-Methods Study of Parents’ Usage and Experiences of eHealth for Self-Management after Hospital Discharge Due to Pediatric Surgery or Preterm Birth
Authors: Lindkvist Rose-Marie , Sjöström-Strand Annica , Landgren Kajsa , Johnsson Björn A. , Stenström Pernilla , Hallström Inger Kristensson
Published: June 2021

The costly and complex needs for children with long-term illness are challenging. Safe eHealth communication is warranted to facilitate health improvement and care services. This mixed-methods study aimed to describe parents’ usage and experiences of communicating with professionals during hospital-to-home-transition after their child’s preterm birth or surgery for colorectal malformations, using an eHealth device, specifically designed for communication and support via nurses at the hospital. The eHealth devices included the possibility for daily reports, video calls, text messaging, and sending images. Interviews with 25 parents were analyzed with qualitative content analysis. Usage data from eHealth devices were compiled from database entries and analyzed statistically. Parents using the eHealth device expressed reduced worry and stress during the initial period at home through effective and safe communication. Benefits described included keeping track of their child’s progress and having easy access to support whenever needed. This was corroborated by usage data indicating that contact was made throughout the day, and more among families living far away from hospital. The eHealth device potentially replaced phone calls and prevented unnecessary visits. The eHealth technique can aid safe self-treatment within child- and family-centered care in neonatal and pediatric surgery treatment. Future research may consider organization perspectives and health economics.
A COVID-19 Exposure at a Dental Clinic Where Healthcare Workers Routinely Use Particulate Filtering Respirators
Authors: Kim Dosup , Ko Jae-Hoon , Peck Kyong Ran , Baek Jin Yang , Moon Hee-Won , Ki Hyun Kyun , Yoon Ji Hyun , Kim Hyo Jin , Choi Jeong Hwa , Park Ga Eun
Published: June 2021

Asymptomatic/mildly symptomatic coronavirus disease 2019 (COVID-19) patients produce a considerable amount of virus and transmit severe acute respiratory syndrome virus 2 (SARS-CoV-2) through close contact. Preventing in-hospital transmission of SARS-CoV-2 is challenging, since symptom-based screening protocols may miss asymptomatic/mildly symptomatic patients. In particular, dental healthcare workers (HCWs) are at high risk of exposure, as face-to-face contact and exposure to oral secretions is unavoidable. We report exposure of HCWs during dental procedures on a mild symptomatic COVID-19 patient. A 32-year-old male visited a dental clinic at a tertiary care hospital. He experienced mild cough, which started three days before the dental visit, but did not report his symptom during the entrance screening. He underwent several dental procedures and imaging for orthognathic surgery without wearing a mask. Seven HCWs were closely exposed to the patient during dental procedures that could have generated droplets and aerosols. One HCW had close contact with the patient during radiologic exams, and seven HCWs had casual contact. All HCWs wore particulate filtering respirators with 94% filter capacity and gloves, but none wore eye protection or gowns. The next day, the patient experienced dysgeusia and was diagnosed with COVID-19 with high viral load. All HCWs who had close contact with the patient were quarantined for 14 days, and polymerase chain reaction and antibody tests for SARS-CoV-2 were negative. This exposure event suggests the protective effect of particulate filtering respirators in dental clinics. The recommendations of different levels of personal protective equipment (PPE) for dental HCWs according to the procedure types should be established according to the planned procedure, the risk of COVID-19 infection of the patient, and the outbreak situation of the community.
Oral Health-Related Quality of Life among Croatian University Students
Authors: Uzarevic Zvonimir , Bulj Ana
Published: June 2021

Oral health-related quality of life (OHRQoL) is utilized in health services research to examine trends in oral health and population-based needs assessment. To determine the impact of oral diseases on everyday life, measures of oral quality of life are needed. In addition to common disease-based measures, they assess the need for oral care to evaluate oral health care programs and treatment management. The aim of this study was to evaluate the OHRQoL among Croatian university students. A cross-sectional study was conducted among 895 students (mean age 22 ± 4 years; 54.75% were females). The data collection was carried out through a self-administered short-form oral health impact profile (OHIP-14) questionnaire which comprises 14 items that describe 7 subscales. Each subscale is named according to its constitutive items: functional limitation, physical pain, psychological discomfort, physical disability, psychological disability, social disability and social handicap. The quality of data was descriptively analysed and internal consistency reliability was assessed by Chronbach’s alpha coefficient. Pearson’s correlation was performed on the OHIP-14 total score and 7 subscale scores. The level of significance was set to p < 0.05. The prevalence of reported impact on OHRQoL was 31.84% and the mean OHIP-14 score was 11.66 ± 8.72. Chronbach’s alpha for the OHIP-14 total score was 0.91 (range for subscales: 0.70–0.87). Total OHIP-14 score was correlated with each of the seven subscales (correlation range: 0.56–0.84). The psychological discomfort and physical pain subscales recorded the highest impact with 56.09% and 24.47%, respectively, while the least impact was recorded in the physical disability subscale with 13.35%. The mean OHIP-14 score of the students in this study reflects that the oral health status of most of the students did not significantly affect their OHRQoL. However, the psychological discomfort and physical pain subscales were the most severely affected aspects of their OHRQoL. The OHIP-14 had reasonable reliability in relation to subjective global oral health indicators among students and thus appears to be a useful OHRQoL measure in this context.
Environmental Tobacco Smoke and Early Language Difficulties among U.S. Children
Authors: Jackson Dylan B. , Testa Alexander
Published: June 2021

Objective: Environmental Tobacco Smoke (ETS) is a serious public health concern with the potential to interfere with various components of healthy child development. Even so, there has been limited nationally representative research investigating these connections. The current study examines the relationship between ETS and language difficulties among toddlers and preschool-aged children in the United States. Method: Data are derived from the 2018 National Survey of Children’s Health and facilitate strategic comparisons between different forms of ETS—namely, children who live with family members who smoke vs. children whose family members smoke inside the housing unit. Results: The findings reveal a robust association between family members smoking inside the housing unit and both receptive and expressive language difficulties, but only among male children. After adjusting for covariates, smoking inside the housing unit is associated with a 182% increase in the rate of early composite language difficulties among male children. These associations persist even when compared to male children who live with smoking family members who do not smoke inside the housing unit. Conclusions: The findings suggest a need for interventions designed to reduce ETS in households with young children and increase targeted language skill training for vulnerable children in an effort to enhance child development and well-being. To maximize this effort, we advocate for interdisciplinary teams, including medical and public health practitioners, educators, and researchers, to work together to develop and implement evidence-based strategies to limit ETS in homes and facilitate healthy language development among young children.
Traffic Density-Related Black Carbon Distribution: Impact of Wind in a Basin Town
Authors: Jereb Borut , Gajšek Brigita , Šipek Gregor , Kovše Špela , Obrecht Matevz
Published: June 2021

Black carbon is one of the riskiest particle matter pollutants that is harmful to human health. Although it has been increasingly investigated, factors that depend on black carbon distribution and concentration are still insufficiently researched. Variables, such as traffic density, wind speeds, and ground levels can lead to substantial variations of black carbon concentrations and potential exposure, which is even riskier for people living in less-airy sites. Therefore, this paper “fills the gaps” by studying black carbon distribution variations, concentrations, and oscillations, with special emphasis on traffic density and road segments, at multiple locations, in a small city located in a basin, with frequent temperature inversions and infrequent low wind speeds. As wind speed has a significant impact on black carbon concentration trends, it is critical to present how low wind speeds influence black carbon dispersion in a basin city, and how black carbon is dependent on traffic density. Our results revealed that when the wind reached speeds of 1 ms−1, black carbon concentrations actually increased. In lengthy wind periods, when wind speeds reached 2 or 3 ms−1, black carbon concentrations decreased during rush hour and in the time of severe winter biomass burning. By observing the results, it could be concluded that black carbon persists longer in higher altitudes than near ground level. Black carbon concentration oscillations were also seen as more pronounced on main roads with higher traffic density. The more the traffic decreases and becomes steady, the more black carbon concentrations oscillate.
Training and Profile of Special Olympics Portugal Coaches: Influence of Formal and Non-Formal Learning
Authors: Pires Pedro , Batista Marco , Marinho Daniel A. , Antúnez Antonio , Mesquita Helena , Ibáñez Sergio J.
Published: June 2021

Since coaches play an important role in the development of athletes, the process and mechanisms used by Special Olympics Portugal to develop coaches’ skills are worthy of research. In this context, the study aims to identify the training paths and profiles of the Special Olympics Portugal coach. It also aims to analyze the relationship between formal and non-formal learning in the profile and training of this type of coach. The research is descriptive and transversal regarding Special Olympics Portugal coaches, with the participation of 50 subjects. Two questionnaires were used, the Coaches’ Training Profile Questionnaire to determine the training routes, and the Coaches’ Orientation Questionnaire. The results show that the Special Olympics Portugal coaches have an academic background and a somewhat critical profile. It is imperative to build formal and non-formal learning contexts that focus on the theme of adapted sports, in order to allow the training of more qualified coaches, who are consequently more effective in their interventions with this type of athlete.
Biomarkers after Controlled Inhalation Exposure to Exhaust from Hydrogenated Vegetable Oil (HVO)
Authors: Krais Annette M. , Essig Julie Y. , Gren Louise , Vogs Carolina , Assarsson Eva , Dierschke Katrin , Nielsen Jörn , Strandberg Bo , Pagels Joakim , Broberg Karin , Lindh Christian H. , Gudmundsson Anders , Wierzbicka Aneta
Published: June 2021

Hydrogenated vegetable oil (HVO) is a renewable diesel fuel used to replace petroleum diesel. The organic compounds in HVO are poorly characterized; therefore, toxicological properties could be different from petroleum diesel exhaust. The aim of this study was to evaluate the exposure and effective biomarkers in 18 individuals after short-term (3 h) exposure to HVO exhaust and petroleum diesel exhaust fumes. Liquid chromatography tandem mass spectrometry was used to analyze urinary biomarkers. A proximity extension assay was used for the measurement of inflammatory proteins in plasma samples. Short-term (3 h) exposure to HVO exhaust (PM1 ~1 µg/m3 and ~90 µg/m3 for vehicles with and without exhaust aftertreatment systems, respectively) did not increase any exposure biomarker, whereas petroleum diesel exhaust (PM1 ~300 µg/m3) increased urinary 4-MHA, a biomarker for p-xylene. HVO exhaust from the vehicle without exhaust aftertreatment system increased urinary 4-HNE-MA, a biomarker for lipid peroxidation, from 64 ng/mL urine (before exposure) to 141 ng/mL (24 h after exposure, p < 0.001). There was no differential expression of plasma inflammatory proteins between the HVO exhaust and control exposure group. In conclusion, short-term exposure to low concentrations of HVO exhaust did not increase urinary exposure biomarkers, but caused a slight increase in lipid peroxidation associated with the particle fraction.
Thermal Imaging of Exercise-Associated Skin Temperature Changes in Swimmers Subjected to 2-min Intensive Exercise on a VASA Swim Bench Ergometer
Authors: Knyszyńska Anna , Radecka Aleksandra , Lubkowska Anna
Published: June 2021

An important element of swimming training is the improvement of muscle strength and the technique of swimming movements on dry land. The heat generated by the muscles involved in the effort contributes to a change in the temperature of the skin surface, which can be assessed by the IRT method. The aim of the study was to assess the symmetry and dynamics of changes in surface temperatures of selected areas of the body in swimmers after exercise on a swimming ergometer with the use of IRT. A total of 12 swimmers (aged 19 ± 1.3 years) completed a two-minute stress test (front crawl swimming movements) using a VASA Swim Ergometer, with a load of 5. Using an IRT camera (FLIR E60), postexercise changes in back and upper limbs surface temperature in relation to the resting values were observed. After exercise, the temperature value of all assessed areas decreased, apparently in the area of the back and the back of the arms, returning to the baseline values after 12 min of observation. There was no asymmetry in mean temperature values between the right and left upper limbs. IRT is a noninvasive and sensitive tool for the individual analysis of changes in body surface temperature in swimmers after training on dry land.
Cigarette Relighting: A Series of Pilot Studies Investigating a Common Yet Understudied Smoking Behavior
Authors: Heckman Carolyn J. , Wackowski Olivia A. , Mukherjee Rohit , Hatsukami Dorothy K. , Stepanov Irina , Delnevo Cristine D. , Steinberg Michael B.
Published: June 2021

Background: The act of extinguishing, saving, and later relighting unfinished cigarettes is a common yet understudied behavior that may have implications for tobacco treatment and health. Methods: This paper presents four pilot studies investigating various aspects of this topic: (1) the prevalence of relighting among NJ and NY Quitline callers (n = 20,984); (2) the prevalence and correlates of relighting in two national surveys (n = 1008, n = 1018); (3) a within-subject (n = 16) laboratory experiment comparing cigarettes smoked per day and exhaled carbon monoxide when relighting and not relighting cigarettes; and (4) a national survey of tobacco treatment providers’ (n = 150) perceptions of relighting. Results: Relighting was found to be common (approximately 45% of smokers), and associated with lower socioeconomic status, heavier smoking and nicotine dependence, greater smoking-related concerns, as well as high levels of exhaled carbon monoxide. Providers noted the potential importance of relighting but reported that they do not regularly incorporate it into their assessment or tobacco treatment planning. Conclusions: These findings address a major research gap in the emerging research on this common behavior. Future research is needed to better understand the potential implications of relighting for policies and clinical practices related to tobacco cessation and health.
Serology in COVID-19: Comparison of Two Methods
Authors: Moniuszko-Malinowska Anna , Jelski Wojciech , Dunaj Justyna , Mroczko Barbara , Czupryna Piotr , Kruszewska Ewelina , Pancewicz Sławomir
Published: June 2021

Background: The aim of our study was to examine the performance of two assays in detecting SARS-CoV-2 antibodies. Methods: A total of 127 COVID-19 disease contacts from the Infectious Diseases Department were included. Two serological tests were used: SARS-CoV-2 IgG CMIA on the Alinity system (Abbott) and LIAISON® SARS-CoV-2 S1/S2 IgG CLIA (DiaSorin). Results: The assays exhibited a 96.85% (123/127 patients) test result agreement. In two cases, the positive results obtained by SARS-CoV-2 IgG CMIA on the Alinity system (Abbott) were negative based on the LIAISON® SARS-CoV-2 S1/S2 IgG CLIA (DiaSorin) test, and in two cases, negative results from the LIAISON® SARS-CoV-2 S1/S2 IgG CLIA (DiaSorin) test were positive with the SARS-CoV-2 IgG CMIA on the Alinity system (Abbott). Conclusions: Based on the results of our study, we conclude that in population medicine, the assessments of anti-SARS-CoV-2 antibodies after exposure to SARS-CoV-2 virus based on spike protein or nucleocapsid protein show comparable effectiveness.
Multimorbidity among People Experiencing Homelessness—Insights from Primary Care Data
Authors: Vallesi Shannen , Tuson Matthew , Davies Andrew , Wood Lisa
Published: June 2021

Background: Although the poor health of people experiencing homelessness is increasingly recognised in health discourse, there is a dearth of research that has quantified the nature and magnitude of chronic health issues and morbidity among people experiencing homelessness, particularly in the Australian context. Methods: Analysis of the medical records of 2068 “active” patients registered with a specialist homeless health service in Perth, Western Australia as of 31 December 2019. Results: Overall, 67.8% of patients had at least one chronic physical health condition, 67.5% had at least one mental health condition, and 61.6% had at least one alcohol or other drug (AOD) use disorder. Nearly half (47.8%) had a dual diagnosis of mental health and AOD use issues, and over a third (38.1%) were tri-morbid (mental health, AOD and physical health condition). Three-quarters (74.9%) were multimorbid or had at least two long-term conditions (LTCs), and on average, each patient had 3.3 LTCs. Conclusions: The study findings have substantial implications from both a health risk and healthcare treatment perspective for people experiencing homeless. The pervasiveness of preventable health conditions among people experiencing homelessness also highlights the imperative to improve the accessibility of public health programs and screening to reduce their morbidity and premature mortality.
Automated Detection and Screening of Traumatic Brain Injury (TBI) Using Computed Tomography Images: A Comprehensive Review and Future Perspectives
Authors: V. Vidhya , Gudigar Anjan , Raghavendra U. , Hegde Ajay , Menon Girish R. , Molinari Filippo , Ciaccio Edward J. , Acharya U. Rajendra
Published: June 2021

Traumatic brain injury (TBI) occurs due to the disruption in the normal functioning of the brain by sudden external forces. The primary and secondary injuries due to TBI include intracranial hematoma (ICH), raised intracranial pressure (ICP), and midline shift (MLS), which can result in significant lifetime disabilities and death. Hence, early diagnosis of TBI is crucial to improve patient outcome. Computed tomography (CT) is the preferred modality of choice to assess the severity of TBI. However, manual visualization and inspection of hematoma and its complications from CT scans is a highly operator-dependent and time-consuming task, which can lead to an inappropriate or delayed prognosis. The development of computer aided diagnosis (CAD) systems could be helpful for accurate, early management of TBI. In this paper, a systematic review of prevailing CAD systems for the detection of hematoma, raised ICP, and MLS in non-contrast axial CT brain images is presented. We also suggest future research to enhance the performance of CAD for early and accurate TBI diagnosis.
Free Fatty Acids Are Associated with the Cognitive Functions in Stroke Survivors
Authors: Kotlęga Dariusz , Peda Barbara , Palma Joanna , Zembroń-Łacny Agnieszka , Gołąb-Janowska Monika , Masztalewicz Marta , Nowacki Przemysław , Szczuko Małgorzata
Published: June 2021

Ischemic stroke is a leading cause of motor impairment and psychosocial disability. Although free fatty acids (FFA) have been proven to affect the risk of stroke and potentially dementia, the evidence of their impact on cognitive functions in stroke patients is lacking. We aimed to establish such potential relationships. Seventy-two ischemic stroke patients were prospectively analysed. Their cognitive functions were assessed seven days post-stroke and six months later as follow-up (n = 41). Seven days post-stroke analysis of serum FFAs levels showed direct correlations between Cognitive Verbal Learning Test (CVLT) and the following FFAs: C20:4n6 arachidonic acid and C20:5n3 eicosapentaenoic acid, while negative correlations were observed for C18:3n3 linolenic acid (ALA), C18:4 n3 stearidonic acid and C23:0 tricosanoic acid. Follow-up examination with CVLT revealed positive correlations with C15:0 pentadecanoid acid, C18:3n6 gamma linoleic acid, SDA, C23:0 tricosanoic acid and negative correlations with C14:0 myristic acid and C14:1 myristolenic acids. Several tests (Trail Making Test, Stroop Dots Trail, Digit Span Test and Verbal Fluency Test) were directly correlated mainly with C14:0 myristic acid and C14:1 myristolenic acid, while corresponding negatively with C18:1 vaccinic acid, C20:3n3 cis-11-eicosatrienoic acid, C22:1/C20:1 cis11- eicosanic acid and C20:2 cis-11-eicodienoic acid. No correlations between Montreal Cognitive Assessment (MOCA) test performed on seventh day, and FFAs levels were found. Saturated fatty acids play a negative role in long-term cognitive outcomes in stroke patients. The metabolic cascade of polyunsaturated fatty acids (n3 PUFA) and the synthesis of (AA) can be involved in pathogenesis of stroke-related cognitive impairment.
Density of Fast Food Outlets around Educational Facilities in Riyadh, Saudi Arabia: Geospatial Analysis
Authors: AlQurashi Alaa Ashraf , Kusuma Dian , AlJishi Hala , AlFaiz Ali , AlSaad Abdulaziz
Published: June 2021

Background: Childhood obesity remains a public health issue globally. The latest estimate from the World Health Organization showed that over 340 million children and adolescents aged 5–19 were overweight or obese in 2016. Objective: Our study aimed to assess the density of fast food outlets around educational facilities in Riyadh, Saudi Arabia. Methods: We employed geospatial and quantitative analyses using data on fast food outlets (from surveys conducted between November 2019 and May 2020) and educational facilities in Riyadh city. Data analyses conducted using ArcMap 10.6 and Stata 15 compared the density within 500 m and 500–1000 m from the facilities. Results: We found a high density of fast food outlets around educational facilities. Nearly 80% of fast food were within twelve-minute walking or five-minute driving distances from schools, and nearly 70% of all educational facilities had at least one fast food outlet within the buffer. We also found the densities were high within both the areas closer and the areas farther away from educational facilities. In addition, the density was significantly higher around private schools compared to government schools, and the density around girls-only schools and both-gender schools was higher than that around boys-only schools. Conclusion: There is a high density of fast food outlets around educational facilities in Saudi Arabia. Effective policies are needed to help reduce potential exposure to fast food among young people in Saudi Arabia and other countries with similar settings.
Molecular Inversion Probe-Based Sequencing of USH2A Exons and Splice Sites as a Cost-Effective Screening Tool in USH2 and arRP Cases
Authors: Reurink Janine , Dockery Adrian , Oziębło Dominika , Farrar G. Jane , Ołdak Monika , ten Brink Jacoline B. , Bergen Arthur A. , Rinne Tuula , Yntema Helger G. , Pennings Ronald J. E. , van den Born L. Ingeborgh , Aben Marco , Oostrik Jaap , Venselaar Hanka , Plomp Astrid S. , Khan M. Imran , van Wijk Erwin , Cremers Frans P. M. , Roosing Susanne , Kremer Hannie
Published: June 2021

A substantial proportion of subjects with autosomal recessive retinitis pigmentosa (arRP) or Usher syndrome type II (USH2) lacks a genetic diagnosis due to incomplete USH2A screening in the early days of genetic testing. These cases lack eligibility for optimal genetic counseling and future therapy. USH2A defects are the most frequent cause of USH2 and are also causative in individuals with arRP. Therefore, USH2A is an important target for genetic screening. The aim of this study was to assess unscreened or incompletely screened and unexplained USH2 and arRP cases for (likely) pathogenic USH2A variants. Molecular inversion probe (MIP)-based sequencing was performed for the USH2A exons and their flanking regions, as well as published deep-intronic variants. This was done to identify single nucleotide variants (SNVs) and copy number variants (CNVs) in 29 unscreened or partially pre-screened USH2 and 11 partially pre-screened arRP subjects. In 29 out of these 40 cases, two (likely) pathogenic variants were successfully identified. Four of the identified SNVs and one CNV were novel. One previously identified synonymous variant was demonstrated to affect pre-mRNA splicing. In conclusion, genetic diagnoses were obtained for a majority of cases, which confirms that MIP-based sequencing is an effective screening tool for USH2A. Seven unexplained cases were selected for future analysis with whole genome sequencing.
Integrated Systems Analysis of Mixed Neuroglial Cultures Proteome Post Oxycodone Exposure
Authors: Guda Rahul S. , Odegaard Katherine E. , Tan Chengxi , Schaal Victoria L. , Yelamanchili Sowmya V. , Pendyala Gurudutt
Published: June 2021

Opioid abuse has become a major public health crisis that affects millions of individuals across the globe. This widespread abuse of prescription opioids and dramatic increase in the availability of illicit opioids have created what is known as the opioid epidemic. Pregnant women are a particularly vulnerable group since they are prescribed for opioids such as morphine, buprenorphine, and methadone, all of which have been shown to cross the placenta and potentially impact the developing fetus. Limited information exists regarding the effect of oxycodone (oxy) on synaptic alterations. To fill this knowledge gap, we employed an integrated system approach to identify proteomic signatures and pathways impacted on mixed neuroglial cultures treated with oxy for 24 h. Differentially expressed proteins were mapped onto global canonical pathways using ingenuity pathway analysis (IPA), identifying enriched pathways associated with ephrin signaling, semaphorin signaling, synaptic long-term depression, endocannabinoid signaling, and opioid signaling. Further analysis by ClueGO identified that the dominant category of differentially expressed protein functions was associated with GDP binding. Since opioid receptors are G-protein coupled receptors (GPCRs), these data indicate that oxy exposure perturbs key pathways associated with synaptic function.
CK1δ-Derived Peptides as Novel Tools Inhibiting the Interactions between CK1δ and APP695 to Modulate the Pathogenic Metabolism of APP
Authors: Roth Aileen , Gärtner Fabian , Mayer Katja , Beyrle Julian , König Irina , Knippschild Uwe , Bischof Joachim
Published: June 2021

Alzheimer’s disease (AD) is the major cause of dementia, and affected individuals suffer from severe cognitive, mental, and functional impairment. Histologically, AD brains are basically characterized by the presence of amyloid plaques and neurofibrillary tangles. Previous reports demonstrated that protein kinase CK1δ influences the metabolism of amyloid precursor protein (APP) by inducing the generation of amyloid-β (Aβ), finally contributing to the formation of amyloid plaques and neuronal cell death. We therefore considered CK1δ as a promising therapeutic target and suggested an innovative strategy for the treatment of AD based on peptide therapeutics specifically modulating the interaction between CK1δ and APP. Initially, CK1δ-derived peptides manipulating the interactions between CK1δ and APP695 were identified by interaction and phosphorylation analysis in vitro. Selected peptides subsequently proved their potential to penetrate cells without inducing cytotoxic effects. Finally, for at least two of the tested CK1δ-derived peptides, a reduction in Aβ levels and amyloid plaque formation could be successfully demonstrated in a complex cell culture model for AD. Consequently, the presented results provide new insights into the interactions of CK1δ and APP695 while also serving as a promising starting point for further development of novel and highly innovative pharmacological tools for the treatment of AD.
(S)-Reutericyclin: Susceptibility Testing and In Vivo Effect on Murine Fecal Microbiome and Volatile Organic Compounds
Authors: Kienesberger Bernhard , Obermüller Beate , Singer Georg , Mittl Barbara , Grabherr Reingard , Mayrhofer Sigrid , Heinl Stefan , Stadlbauer Vanessa , Horvath Angela , Miekisch Wolfram , Fuchs Patricia , Klymiuk Ingeborg , Till Holger , Castellani Christoph
Published: June 2021

We aimed to assess the in vitro antimicrobial activity and the in vivo effect on the murine fecal microbiome and volatile organic compound (VOC) profile of (S)-reutericyclin. The antimicrobial activity of (S)-reutericyclin was tested against Clostridium difficile, Listeria monocytogenes, Escherichia coli, Enterococcus faecium, Staphylococcus aureus, Staphylococcus (S.) epidermidis, Streptococcus agalactiae, Pseudomonas aeruginosa and Propionibacterium acnes. Reutericyclin or water were gavage fed to male BALBc mice for 7 weeks. Thereafter stool samples underwent 16S based microbiome analysis and VOC analysis by gas chromatography mass spectrometry (GC-MS). (S)-reutericyclin inhibited growth of S. epidermidis only. Oral (S)-reutericyclin treatment caused a trend towards reduced alpha diversity. Beta diversity was significantly influenced by reutericyclin. Linear discriminant analysis Effect Size (LEfSe) analysis showed an increase of Streptococcus and Muribaculum as well as a decrease of butyrate producing Ruminoclostridium, Roseburia and Eubacterium in the reutericyclin group. VOC analysis revealed significant increases of pentane and heptane and decreases of 2,3-butanedione and 2-heptanone in reutericyclin animals. The antimicrobial activity of (S)-reutericyclin differs from reports of (R)-reutericyclin with inhibitory effects on a multitude of Gram-positive bacteria reported in the literature. In vivo (S)-reutericyclin treatment led to a microbiome shift towards dysbiosis and distinct alterations of the fecal VOC profile.
Culture Medium and Sex Drive Epigenetic Reprogramming in Preimplantation Bovine Embryos
Authors: Canovas Sebastian , Ivanova Elena , Hamdi Meriem , Perez-Sanz Fernando , Rizos Dimitrios , Kelsey Gavin , Coy Pilar
Published: June 2021

Assisted reproductive technologies impact transcriptome and epigenome of embryos and can result in long-term phenotypic consequences. Whole-genome DNA methylation profiles from individual bovine blastocysts in vivo- and in vitro-derived (using three sources of protein: reproductive fluids, blood serum and bovine serum albumin) were generated. The impact of in vitro culture on DNA methylation was analyzed, and sex-specific methylation differences at blastocyst stage were uncovered. In vivo embryos showed the highest levels of methylation (29.5%), close to those produced in vitro with serum, whilst embryos produced in vitro with reproductive fluids or albumin showed less global methylation (25–25.4%). During repetitive element analysis, the serum group was the most affected. DNA methylation differences between in vivo and in vitro groups were more frequent in the first intron than in CpGi in promoters. Moreover, hierarchical cluster analysis showed that sex produced a stronger bias in the results than embryo origin. For each group, distance between male and female embryos varied, with in vivo blastocyst showing a lesser distance. Between the sexually dimorphic methylated tiles, which were biased to X-chromosome, critical factors for reproduction, developmental process, cell proliferation and DNA methylation machinery were included. These results support the idea that blastocysts show sexually-dimorphic DNA methylation patterns, and the known picture about the blastocyst methylome should be reconsidered.
Piezo Channels: Awesome Mechanosensitive Structures in Cellular Mechanotransduction and Their Role in Bone
Authors: Xu Xia , Liu Shuyu , Liu Hua , Ru Kang , Jia Yunxian , Wu Zixiang , Liang Shujing , Khan Zarnaz , Chen Zhihao , Qian Airong , Hu Lifang
Published: June 2021

Piezo channels are mechanosensitive ion channels located in the cell membrane and function as key cellular mechanotransducers for converting mechanical stimuli into electrochemical signals. Emerged as key molecular detectors of mechanical forces, Piezo channels’ functions in bone have attracted more and more attention. Here, we summarize the current knowledge of Piezo channels and review the research advances of Piezo channels’ function in bone by highlighting Piezo1′s role in bone cells, including osteocyte, bone marrow mesenchymal stem cell (BM-MSC), osteoblast, osteoclast, and chondrocyte. Moreover, the role of Piezo channels in bone diseases is summarized.