bluuebunny/crossref_metadata_2025 huggingface dataset:
Influence of Electron Beam Welding Parameters on the Microstructure Formation and Mechanical Behaviors of the Ti and Ni Dissimilar Metals Welded Joints
Authors: Šimeková Beáta , Hodúlová Erika , Kovačócy Pavel , Kovaříková Ingrid , Sahul Miroslav , Sahul Martin , Pašák Matej , Pixner Florian
Published: May 2022

Commercially pure titanium Ti Grade 2, 2 mm in thickness, was welded to 2 mm thick nickel alloy 201 with electron beam welding. Various welding parameters were used to create the butt-welded joints. The innovation herein consists of welding two dissimilar metals that are declared non-weldable. The welding current used for electron beam welding was 40–70 mA and welding speeds were 20–50 mm/s. In this experiment, we tested two offsets of the electron beam, which were 100–300 μm to the nickel side and 200 μm to the titanium side. It was observed that the offset of the beam had no effect on the weld joint’s strength. The samples were subjected to a visual test in which longitudinal and transverse cracks were recorded along the whole weld. Only four samples retained the integrity of the joint. Microstructures of the weld joints were examined by scanning confocal and scanning electron microscopy. Energy dispersive spectroscopy (EDS) analysis confirmed the phase constitution inside the weld regions and the fusion interfaces. Tensile strength and microhardness tests were used to evaluate the mechanical parameters of the Ti/Ni welded joint. The results showed that cracking of brittle Ni–Ti intermetallic phases in electron beam welded joints occurred. The microstructure in the fusion zone’s center part was primarily NiTi and Ti2Ni. No clear correlation was found between heat input or welding parameters—welding current and welding speed—and tensile strength. The strain–tensile strength curve resulted in brittle fracturing. The hardness of the weld zone was five times higher than that of the base metal and heat-affected zone. The amount of heat input into the welded metal is as critical as the large asymmetry in heat transport that controls the process of solidification from each side of the base metal.
Impact of Nitisinone on the Cerebrospinal Fluid Metabolome of a Murine Model of Alkaptonuria
Authors: Davison Andrew S. , Norman Brendan P. , Sutherland Hazel , Milan Anna M. , Gallagher James A. , Jarvis Jonathan C. , Ranganath Lakshminarayan R.
Published: May 2022

Background: Nitisinone-induced hypertyrosinaemia is well documented in Alkaptonuria (AKU), and there is uncertainty over whether it may contribute to a decline in cognitive function and/or mood by altering neurotransmitter metabolism. The aim of this work was to evaluate the impact of nitisinone on the cerebrospinal fluid (CSF) metabolome in a murine model of AKU, with a view to providing additional insight into metabolic changes that occur following treatment with nitisinone. Methods: 17 CSF samples were collected from BALB/c Hgd−/− mice (n = 8, treated with nitisinone—4 mg/L and n = 9, no treatment). Samples were diluted 1:1 with deionised water and analysed using a 1290 Infinity II liquid chromatography system coupled to a 6550 quadrupole time-of-flight mass spectrometry (Agilent, Cheadle, UK). Raw data were processed using a targeted feature extraction algorithm and an established in-house accurate mass retention time database. Matched entities (±10 ppm theoretical accurate mass and ±0.3 min retention time window) were filtered based on their frequency and variability. Experimental groups were compared using a moderated t-test with Benjamini–Hochberg false-discovery rate adjustment. Results: L-Tyrosine, N-acetyl-L-tyrosine, γ-glutamyl-L-tyrosine, p-hydroxyphenylacetic acid, and 3-(4-hydroxyphenyl)lactic acid were shown to increase in abundance (log2 fold change 2.6–6.9, 3/5 were significant p < 0.05) in the mice that received nitisinone. Several other metabolites of interest were matched, but no significant differences were observed, including the aromatic amino acids phenylalanine and tryptophan, and monoamine metabolites adrenaline, 3-methoxy-4-hydroxyphenylglycol, and octopamine. Conclusions: Evaluation of the CSF metabolome of a murine model of AKU revealed a significant increase in the abundance of a limited number of metabolites following treatment with nitisinone. Further work is required to understand the significance of these findings and the mechanisms by which the altered metabolite abundances occur.
Molecular Communication between Plants and Plant-Growth-Promoting Microorganisms for Stress Tolerance
Authors: Khan Naeem
Published: May 2022

Plant-growth-promoting microorganisms are beneficial microbes that reside in the rhizosphere and roots of plants, direct their developmental process and induce systemic resistance [...]
An In Silico Analysis of Malaria Pre-Erythrocytic-Stage Antigens Interpreting Worldwide Genetic Data to Suggest Vaccine Candidate Variants and Epitopes
Authors: Ouattara Amed , Dwivedi Ankit , Adams Matthew , Niangaly Amadou , Laurens Matthew B. , Nyunt Myaing M. , Plowe Christopher V. , Djimde Abdoulaye , Takala-Harrison Shannon , Silva Joana C.
Published: May 2022

Failure to account for genetic diversity of antigens during vaccine design may lead to vaccine escape. To evaluate the vaccine escape potential of antigens used in vaccines currently in development or clinical testing, we surveyed the genetic diversity, measured population differentiation, and performed in silico prediction and analysis of T-cell epitopes of ten such Plasmodium falciparum pre-erythrocytic-stage antigens using whole-genome sequence data from 1010 field isolates. Of these, 699 were collected in Africa (Burkina Faso, Cameroon, Guinea, Kenya, Malawi, Mali, and Tanzania), 69 in South America (Brazil, Colombia, French Guiana, and Peru), 59 in Oceania (Papua New Guinea), and 183 in Asia (Cambodia, Myanmar, and Thailand). Antigens surveyed include cell-traversal protein for ookinetes and sporozoites, circumsporozoite protein, liver-stage antigens 1 and 3, sporozoite surface proteins P36 and P52, sporozoite asparagine-rich protein-1, sporozoite microneme protein essential for cell traversal-2, and upregulated-in-infectious-sporozoite 3 and 4 proteins. The analyses showed that a limited number of these protein variants, when combined, would be representative of worldwide parasite populations. Moreover, predicted T-cell epitopes were identified that could be further explored for immunogenicity and protective efficacy. Findings can inform the rational design of a multivalent malaria vaccine.
Study on the Alteration of Pore Parameters of Shale with Different Natural Fractures under Supercritical Carbon Dioxide Seepage
Authors: Tao Lei , Han Jian , Feng Yanjun , McLennan John D.
Published: May 2022

Supercritical CO2 can reduce formation fracture pressure, form more complex fractures in the near-well zone, and replace methane to complete carbon sequestration, which is an important direction for the efficient development of deep shale gas with carbon sequestration. In this paper, based on the scCO2 fracturing field test parameters and the characteristics of common shale calcite filled natural fractures, we simulated the porosity change in shale with three kinds of fractures (no fracture, named NF; axial natural fracture, named AF; and transversal natural fracture, named TF) under scCO2 seepage, and carried out the experimental verification of shale under supercritical CO2 seepage. It was found that: (1) At the same pressure, when the temperature is greater than the critical temperature, the shale porosity of three kinds of fractures gradually increases with the injection of CO2, and the higher the temperature, the more obvious the increase in porosity. (2) At the same temperature and different pressures, the effect of pressure change on the porosity of shale specimens was more obvious than that of temperature. (3) Multi-field coupling experiments of shale under supercritical CO2 seepage revealed that the porosity of all three shale specimens at the same temperature and pressure increased after CO2 injection, and the relative increase in shale porosity measured experimentally was basically consistent with the numerical simulation results. This paper reveals the mechanism of the effect of different temperatures and pressures of scCO2 and different natural fractures on the change in shale porosity, which can be used to optimize the CO2 injection in supercritical CO2 fracturing and carbon sequestration.
Nano-Silica Modified with Diamine for Capturing Azo Dye from Aqueous Solutions
Authors: Da’na Enshirah
Published: May 2022

Nano-silica particles decorated with amine groups (S-DA) were prepared via a simple, one-pot method, and under very mild conditions in an attempt to improve the affinity of the silica nanoparticles toward capturing anionic organic dye, namely, methyl orange (MO). The prepared sample was characterized by different techniques such as XRD for crystallinity, SEM for morphological structure, TGA for thermal stability, BET surface area, and FTIR for surface functional groups. The prepared sample was tested for the removal of MO under different conditions including the mass of adsorbent, pH, initial concentration, and time. Results showed that the adsorption of MO was very fast with equilibrium achieved in less than 30 min and a maximum removal efficiency of 100% for a mass to volume ratio of 10 g/3 L, a pH of 2.5, initial concentration of 10 mgL−1, and under stagnant conditions. These results were compared with a bare nano-silica, which was not able to adsorb more than 3% after 24 h, indicating the important effect of amine groups. Furthermore, recycling the adsorbent was achieved by rinsing the MO-loaded adsorbent with a dilute solution of KOH. The adsorbent maintained 50% of its initial removal efficiency after four adsorption–desorption cycles.
Copper Binding and Oligomerization Studies of the Metal Resistance Determinant CrdA from Helicobacter pylori
Authors: Kekez Ivana , Faletar Mihovil , Kekez Mario , Cendron Laura , Wright Maya , Zanotti Giuseppe , Matković-Čalogović Dubravka
Published: May 2022

Within this research, the CrdA protein from Helicobacter pylori (HpCrdA), a putative copper-binding protein important for the survival of bacterium, was biophysically characterized in a solution, and its binding affinity toward copper was experimentally determined. Incubation of HpCrdA with Cu(II) ions favors the formation of the monomeric species in the solution. The modeled HpCrdA structure shows a conserved methionine-rich region, a potential binding site for Cu(I), as in the structures of similar copper-binding proteins, CopC and PcoC, from Pseudomonas syringae and from Escherichia coli, respectively. Within the conserved amino acid motif, HpCrdA contains two additional methionines and two glutamic acid residues (MMXEMPGMXXMXEM) in comparison to CopC and PcoC but lacks the canonical Cu(II) binding site (two His) since the sequence has no His residues. The methionine-rich site is in a flexible loop and can adopt different geometries for the two copper oxidation states. It could bind copper in both oxidation states (I and II), but with different binding affinities, micromolar was found for Cu(II), and less than nanomolar is proposed for Cu(I). Considering that CrdA is a periplasmic protein involved in chaperoning copper export and delivery in the H. pylori cell and that the affinity of the interaction corresponds to a middle or strong metal–protein interaction depending on the copper oxidation state, we conclude that the interaction also occurs in vivo and is physiologically relevant for H. pylori.
Response Surface Optimisation of Polydimethylsiloxane (PDMS) on Borosilicate Glass and Stainless Steel (SS316) to Increase Hydrophobicity
Authors: Ramlan Nadiah , Zubairi Saiful Irwan , Maskat Mohamad Yusof
Published: May 2022

Particle deposition on the surface of a drying chamber is the main drawback in the spray drying process, reducing product recovery and affecting the quality of the product. In view of this, the potential application of chemical surface modification to produce a hydrophobic surface that reduces the powder adhesion (biofouling) on the wall of the drying chamber is investigated in this study. A hydrophobic polydimethylsiloxane (PDMS) solution was used in the vertical dipping method at room temperature to determine the optimum coating parameters on borosilicate glass and stainless steel substrates, which were used to mimic the wall surface of the drying chamber, to achieve highly hydrophobic surfaces. A single-factor experiment was used to define the range of the PDMS concentration and treatment duration using the Response Surface Methodology (RSM). The Central Composite Rotatable Design (CCRD) was used to study the effects of the concentration of the PDMS solution (X1, %) and the treatment duration (X2, h) on the contact angle of the substrates (°), which reflected the hydrophobicity of the surface. A three-dimensional response surface was constructed to examine the influence of the PDMS concentration and treatment duration on contact angle readings, which serve as an indicator of the surface’s hydrophobic characteristics. Based on the optimisation study, the PDMS coating for the borosilicate glass achieved an optimum contact angle of 99.33° through the combination of a PDMS concentration of X1 = 1% (w/v) and treatment time of X2 = 4.94 h, while the PDMS coating for the stainless steel substrate achieved an optimum contact angle of 98.31° with a PDMS concentration of X1 = 1% (w/v) and treatment time of X2 = 1 h. Additionally, the infrared spectra identified several new peaks that appeared on the PDMS-treated surfaces, which represented the presence of Si-O-Si, Si-CH3, CH2, and CH3 functional groups for the substrates coated with PDMS. Furthermore, the surface morphology analysis using the Field Emission Scanning Electron Microscopy (FESEM) showed the presence of significant roughness and a uniform nanostructure on the surface of the PDMS-treated substrates, which indicates the reduction in wettability and the potential effect of unwanted biofouling on the spray drying chamber. The application of PDMS and PTFE on the optimally coated substrates successfully reduced the amount of full cream milk particles that adhered to the surface. The low surface energy of the treated surface (19–27 mJ/m2) and the slightly higher surface tension of the full cream milk (54–59 mJ/m2) resulted in a high contact angle (102–103°) and reduced the adhesion work on the treated substrates (41–46 mJ/m2) as compared to the native substrates.
The Role of Bile Acids in the Human Body and in the Development of Diseases
Authors: Shulpekova Yulia , Zharkova Maria , Tkachenko Pyotr , Tikhonov Igor , Stepanov Alexander , Synitsyna Alexandra , Izotov Alexander , Butkova Tatyana , Shulpekova Nadezhda , Lapina Natalia , Nechaev Vladimir , Kardasheva Svetlana , Okhlobystin Alexey , Ivashkin Vladimir
Published: May 2022

Bile acids are specific and quantitatively important organic components of bile, which are synthesized by hepatocytes from cholesterol and are involved in the osmotic process that ensures the outflow of bile. Bile acids include many varieties of amphipathic acid steroids. These are molecules that play a major role in the digestion of fats and the intestinal absorption of hydrophobic compounds and are also involved in the regulation of many functions of the liver, cholangiocytes, and extrahepatic tissues, acting essentially as hormones. The biological effects are realized through variable membrane or nuclear receptors. Hepatic synthesis, intestinal modifications, intestinal peristalsis and permeability, and receptor activity can affect the quantitative and qualitative bile acids composition significantly leading to extrahepatic pathologies. The complexity of bile acids receptors and the effects of cross-activations makes interpretation of the results of the studies rather difficult. In spite, this is a very perspective direction for pharmacology.
Seed Priming with Devosia sp. Cell-Free Supernatant (CFS) and Citrus Bioflavonoids Enhance Canola and Soybean Seed Germination
Authors: Shah Ateeq , Subramanian Sowmyalakshmi , Smith Donald L.
Published: May 2022

Climate change, environmental pollution and associated abiotic stresses are beginning to meaningfully affect agricultural production worldwide. Salt stress is, however, one of the most important threats that significantly impairs plant growth and development. Plants in their early growth stages such as seed germination, seed emergence and early seedling growth are very sensitive to salt stress. Among the range of sustainable techniques adopted to improve seed germination and early plant growth is seed priming; however, with the use of ecofriendly substances, this is one of the most effective and economically viable techniques to improve seed tolerance against such environmental stresses. For instance, priming with appropriate non-synthetic compounds including microbial biostimulants are prominent ways to sustainably address these challenges. Therefore, in this research, by using the “priming technique”, two biostimulants were tested for their potential as sustainable approaches to improve canola and soybean seed germination under salt stress and optimal growth conditions. Canola and soybean seeds were primed with flavonoids extracted from citrus fruits (flavopriming) and cell-free supernatant (CFS; produced by a novel strain of Devosia sp.—SL43), alone and in combination, and exposed to low–higher levels of salt stress and ideal growth conditions. Both biostimulants showed promising effects by significantly improving seed germination of soybean and canola under both ideal and stressful conditions. However, increases in seed germination were greater under salinity stress as flavonoids and CFS with stress amelioration effects showed substantial and statistically significant improvements in seed germination under varying levels of salt stress. In addition, combinations (mixtures) of both biostimulants were tested to determine if their effects might be more additive or multiplicative than the individual applications. However, results suggested incompatibility of both biostimulants as none of the combinations showed better results than that of the individual applications of either flavonoids or CFS. Conceivably, the use of flavonoids and this novel Devosia sp. CFS could be significant plant growth enhancers, perhaps much better than the few other biostimulants and bacterial-based compounds currently in use.
A Review of Twenty Years of Research on the Regulation of Signaling Pathways by Natural Products in Breast Cancer
Authors: Naeem Muhammad , Iqbal Muhammad Omer , Khan Humaira , Ahmed Muhammad Masood , Farooq Muhammad , Aadil Muhammad Moeen , Jamaludin Mohamad Ikhwan , Hazafa Abu , Tsai Wan-Chi
Published: May 2022

Breast cancer (BC) is the second leading cause of death among women, and it has become a global health issue due to the increasing number of cases. Different treatment options, including radiotherapy, surgery, chemotherapy and anti-estrogen therapy, aromatase inhibitors, anti-angiogenesis drugs, and anthracyclines, are available for BC treatment. However, due to its high occurrence and disease progression, effective therapeutic options for metastatic BC are still lacking. Considering this scenario, there is an urgent need for an effective therapeutic strategy to meet the current challenges of BC. Natural products have been screened as anticancer agents as they are cost-effective, possess low toxicity and fewer side effects, and are considered alternative therapeutic options for BC therapy. Natural products showed anticancer activities against BC through the inhibition of angiogenesis, cell migrations, proliferations, and tumor growth; cell cycle arrest by inducing apoptosis and cell death, the downstream regulation of signaling pathways (such as Notch, NF-κB, PI3K/Akt/mTOR, MAPK/ERK, and NFAT-MDM2), and the regulation of EMT processes. Natural products also acted synergistically to overcome the drug resistance issue, thus improving their efficacy as an emerging therapeutic option for BC therapy. This review focused on the emerging roles of novel natural products and derived bioactive compounds as therapeutic agents against BC. The present review also discussed the mechanism of action through signaling pathways and the synergistic approach of natural compounds to improve their efficacy. We discussed the recent in vivo and in vitro studies for exploring the overexpression of oncogenes in the case of BC and the current status of newly discovered natural products in clinical investigations.
Phytochemical Compositions of Some Red Sea Halophyte Plants with Antioxidant and Anticancer Potentials
Authors: Hawas Usama W. , El-Kassem Lamia T. Abou , Shaher Fekri M. , Al-Farawati Radwan , Ghandourah Mohamed
Published: May 2022

The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album. The results showed that S. aegyptiaca and S. monoica were rich in gallic acid with 41.72 and 47.48 mg/g, respectively, while A. ehrenbergii was rich in naringenin with 11.88 mg/g. The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album. Glutamic acid and aspartic acid were the major AAs in all halophyte species with 50–63% and 10–22% of the total AAs, respectively. The proportion of unsaturated fatty acids (UFA) of the four species was 42.18–55.33%, comprised mainly of linolenic acid (15.54–28.63%) and oleic acid (5.68–22.05%), while palmitic acid (23.94–49.49%) was the most abundant saturated fatty acid (SFA). Phytol and 9,19-cyclolanost-24-en-3β–ol represented the major unsaponifiable matter (USM) constituents of S. monoica and A. ehrenbergii with proportions 42.44 and 44.11%, respectively. The phenolic fraction of S. aegyptiaca and S. monoica demonstrated noteworthy antioxidant activity with IC50 values of 9.0 and 8.0 μg/mL, respectively, while the FAs fraction of Z. album exhibited potent cytotoxic activity against Huh-7, A-549, and Caco-2 cancer cell lines with IC50 values of 7.4, 10.8, and 11.8 μg/mL, respectively. Our results indicate that these plants may be considered a source of naturally occurring compounds with antioxidant and anticancer effects that could be suitable for future applications.
Study of Degradation Mechanisms of Strength and Thermal-Physical Properties of Nitride and Carbide Ceramics—Promising Materials for Nuclear Energy
Authors: Berguzinov Askhat , Kozlovskiy Artem , Kenzhina Inesh , Shlimas Dmitriy I.
Published: May 2022

The dependences of changes in the strength properties of nitride and carbide ceramics under high temperature irradiation with Kr15+ and Xe22+ heavy ions at irradiation doses of 1012–1015 ions/cm2 are presented in this work. The irradiation was chosen to simulate radiation damage processes that are closest to the real conditions of reactor tests in operating modes of increased temperatures. Polycrystalline ceramics based on AlN, Si3N4 nitrides, and SiC carbides were chosen as objects of research, as they have great prospects for use as a basis for structural materials for high-temperature nuclear reactors, as well as materials for nuclear waste disposal. During these studies the effect of radiation damage caused by irradiation with different fluences on the change in mechanical strength and hardness were determined, and the mechanisms causing these changes depending on the type of irradiated materials were proposed. The novelty of this study is in the results obtained determining the stability of the strength and thermophysical parameters of nitride and carbide ceramics exposed to high-temperature irradiation, which made it possible to determine the main stages and mechanisms for changing these parameters depending on the accumulated radiation damage. The relevance of this study consists not only in obtaining new data on the properties of structural materials exposed to ionizing radiation, but also in the possibility of determining the mechanisms of radiation damage in ceramics.
Photocatalytic Reduction of CO2 with N-Doped TiO2-Based Photocatalysts Obtained in One-Pot Supercritical Synthesis
Authors: Andrade Óscar , Rodríguez Verónica , Camarillo Rafael , Martínez Fabiola , Jiménez Carlos , Rincón Jesusa
Published: May 2022

The objective of this work was to analyze the effect of carbon support on the activity and selectivity of N-doped TiO2 nanoparticles. Thus, N-doped TiO2 and two types of composites, N-doped TiO2/CNT and N-doped TiO2/rGO, were prepared by a new environmentally friendly one-pot method. CNT and rGO were used as supports, triethylamine and urea as N doping agents, and titanium (IV) tetraisopropoxide and ethanol as Ti precursor and hydrolysis agent, respectively. The as-prepared photocatalysts exhibited enhanced photocatalytic performance compared to TiO2 P25 commercial catalyst during the photoreduction of CO2 with water vapor. It was imputed to the synergistic effect of N doping (reduction of semiconductor band gap energy) and carbon support (enlarging e−-h+ recombination time). The activity and selectivity of catalysts varied depending on the investigated material. Thus, whereas N-doped TiO2 nanoparticles led to a gaseous mixture, where CH4 formed the majority compared to CO, N-doped TiO2/CNT and N-doped TiO2/rGO composites almost exclusively generated CO. Regarding the activity of the catalysts, the highest production rates of CO (8 µmol/gTiO2/h) and CH4 (4 µmol/gTiO2/h) were achieved with composite N1/TiO2/rGO and N1/TiO2 nanoparticles, respectively, where superscript represents the ratio mg N/g TiO2. These rates are four times and almost forty times higher than the CO and CH4 production rates observed with commercial TiO2 P25.
Hybrid Nanofluid Flow Induced by an Oscillating Disk Considering Surface Catalyzed Reaction and Nanoparticles Shape Factor
Authors: Ramzan Muhammad , Riasat Saima , Aljurbua Saleh Fahad , Ghazwani Hassan Ali S. , Mahmoud Omar
Published: May 2022

Lately, a new class of nanofluids, namely hybrid nanofluids, has been introduced that performs much better compared with the nanofluids when a healthier heat transfer rate is the objective of the study. Heading in the same direction, the present investigation accentuates the unsteady hybrid nanofluid flow involving CuO, Al2O3/C2H6O2 achieved by an oscillating disk immersed in the porous media. In a study of the homogeneous and heterogeneous reactions, the surface catalyzed reaction was also considered to minimize the reaction time. The shape factors of the nanoparticles were also taken into account, as these play a vital role in assessing the thermal conductivity and heat transfer rate of the system. The assumed model is presented mathematically in the form of partial differential equations. The system is transformed by invoking special similarity transformations. The Keller Box scheme was used to obtain numerical and graphical results. It is inferred that the blade-shaped nanoparticles have the best thermal conductivity that boosts the heat transfer efficiency. The oscillation and surface-catalyzed chemical reactions have opposite impacts on the concentration profile. This analysis also includes a comparison of the proposed model with a published result in a limiting case to check the authenticity of the presented model.
Disruption of Glutamate Homeostasis in the Brain of Rat Offspring Induced by Prenatal and Early Postnatal Exposure to Maternal High-Sugar Diet
Authors: Mizera Jozef , Pomierny Bartosz , Sadakierska-Chudy Anna , Bystrowska Beata , Pomierny-Chamiolo Lucyna
Published: May 2022

A high-calorie diet has contributed greatly to the prevalence of overweight and obesity worldwide for decades. These conditions also affect pregnant women and have a negative impact on the health of both the woman and the fetus. Numerous studies indicate that an unbalanced maternal diet, rich in sugars and fats, can influence the in utero environment and, therefore, the future health of the child. It has also been shown that prenatal exposure to an unbalanced diet might permanently alter neurotransmission in offspring. In this study, using a rat model, we evaluated the effects of a maternal high-sugar diet on the level of extracellular glutamate and the expression of key transporters crucial for maintaining glutamate homeostasis in offspring. Glutamate concentration was assessed in extracellular fluid samples collected from the medial prefrontal cortex and hippocampus of male and female offspring. Analysis showed significantly increased glutamate levels in both brain structures analyzed, regardless of the sex of the offspring. These changes were accompanied by altered expression of the EAAT1, VGLUT1, and xc− proteins in these brain structures. This animal study further confirms our previous findings that a maternal high-sugar diet has a detrimental effect on the glutamatergic system.
Intergenerational Transmission of Obesity from Mothers to Their Offspring: Trends and Associated Factors Derived from the Malaysian National Health and Morbidity Survey (NHMS)
Authors: Mohamed Nur Nadia , Rohana Abdul Jalil , Hamid Noor Aman A , Hu Frank B. , Malik Vasanti S. , Mohd Yusoff Muhammad Fadhli , Aris Tahir ,   The Global Nutrition and Epidemiologic Transition Initiative (GNET)
Published: May 2022

Along with the increasing overweight and obesity trends among adults and children globally, numerous studies have suggested a strong association between maternal overweight and obesity among their offspring. We sought to report the prevalence and associated factors of intergenerational overweight and obesity among mother–child pairs in Malaysia from 2006 to 2015. Data were analysed from three waves of the Malaysian National Health and Morbidity Survey, a population-based cross-sectional study conducted in 2006, 2011 and 2015. A mother and the youngest child from each household formed ‘mother–child pairs’ and were grouped according to their body mass index categories. A multivariable logistic regression model was performed to determine the factors associated with overweight mother/overweight child pairs (OWM/OWC), with normal weight mother/normal weight child pairs (NWM/NWC) as the reference group. The prevalence of OWM/OWC increased from 15.3% to 21.7%, while the prevalence of NWM/NWC decreased from 28.4% to 23.8% between 2006 and 2015. Older maternal age and having primary and secondary education levels were positively associated with OWM/OWC. Conversely, older child age, Chinese ethnicity, large household size and low-income households were inversely associated with OWM/OWC. In conclusion, intergenerational weight gain is a worrisome trend in Malaysia. These findings may help in guiding priority setting for obesity prevention strategies in Malaysia.
Mixed Milk Feeding: A New Approach to Describe Feeding Patterns in the First Year of Life Based on Individual Participant Data from Two Randomised Controlled Trials
Authors: Papadopoulos Nikolaos G. , Balan Theodor A. , van der Merwe Liandre F. , Pang Wei Wei , Michaelis Louise J. , Shek Lynette P. , Vandenplas Yvan , Teoh Oon Hoe , Fiocchi Alessandro G. , Chong Yap Seng ,   on behalf of the TEMPO Study Group,   on behalf of the Venus Study Group
Published: May 2022

‘Mixed Milk Feeding’ (MMF), whereby infants are fed with both breastmilk and infant formula during the same period, is a common feeding practice. Despite its high prevalence, knowledge regarding MMF practices and their association with (health) outcomes is limited, potentially because MMF behaviours are highly variable and difficult to standardise longitudinally. In this paper, we applied a statistical clustering algorithm on individual infant feeding data collected over the first year of life from two clinical trials: ‘TEMPO’ (n = 855) and ‘Venus’ (n = 539); these studies were conducted in different years and world regions. In TEMPO, more than half of infants were MMF. Four distinct MMF clusters were identified: early exclusive formula feeding (32%), later exclusive formula feeding (25%), long-term MMF (21%), and mostly breastfeeding (22%). The same method applied to ‘Venus’ resulted in comparable clusters, building trust in the robustness of the cluster approach. These results demonstrate that distinct MMF patterns can be identified, which may be applicable to diverse populations. These insights could support the design of future research studying the impact of infant feeding patterns on health outcomes. To standardise this in future research, it is important to establish a unified definition of MMF.
The Efficacy of Fish as an Early Complementary Food on the Linear Growth of Infants Aged 6–7 Months: A Randomised Controlled Trial
Authors: Chipili Given , Van Graan Averalda , Lombard Carl J. , Van Niekerk Evette
Published: May 2022

Fish is a good animal-source protein for growth and development. The main objective of the study was to assess the efficacy of fish during the early complementary feeding period on infants’ linear growth in the Samfya district of the Luapula Province of Zambia in 6 months randomised controlled trial. The study was conducted from April 2019 to January 2020. Infants aged 6–7 months (N = 238) were assigned to either the intervention (treatment) group or control (placebo) group to receive fish powder or sorghum powder, respectively. Participants were followed on a weekly basis to distribute the powder and record compliance/usage and any morbidities. Anthropometric measurements were taken monthly. A linear mixed-effects model showed that fish powder improved linear growth among infants over all the 6 months of the intervention period. The fish powder increased length-for-age z scores by 1.26 (95% CI: 0.94–1.57) and weight-for-age z score by 0.95 (95% CI 0.6–1.23). The addition of fish powder to the infant’s usual food during the early complementary feeding improves the infant’s linear growth outcome.
Bacteroides fragilis Supplementation Deteriorated Metabolic Dysfunction, Inflammation, and Aorta Atherosclerosis by Inducing Gut Microbiota Dysbiosis in Animal Model
Authors: Shi Guoxiang , Lin Yubi , Wu Yuanyuan , Zhou Jing , Cao Lixiang , Chen Jiyan , Li Yong , Tan Ning , Zhong Shilong
Published: May 2022

Background: The gut microbial ecosystem is an important factor that regulates host health and the onset of chronic diseases, such as inflammatory bowel diseases, obesity, hyperlipidemia, and diabetes mellitus, which are important risk factors for atherosclerosis. However, the links among diet, microbiota composition, and atherosclerotic progression are unclear. Methods and results: Four-week-old mice (-/- mice, C57Bl/6) were randomly divided into two groups, namely, supplementation with culture medium (control, CTR) and Bacteroides fragilis (BFS), and were fed a high-fat diet. The gut microbiota abundance in feces was evaluated using the 16S rDNA cloning library construction, sequencing, and bioinformatics analysis. The atherosclerotic lesion was estimated using Oil Red O staining. Levels of CD36, a scavenger receptor implicated in atherosclerosis, and F4/80, a macrophage marker in small intestine, were quantified by quantitative real-time PCR. Compared with the CTR group, the BFS group showed increased food intake, fasting blood glucose level, body weight, low-density lipoprotein level, and aortic atherosclerotic lesions. BFS dramatically reduced Lactobacillaceae (LAC) abundance and increased Desulfovibrionaceae (DSV) abundance. The mRNA expression levels of CD36 and F4/80 in small intestine and aorta tissue in the BFS group were significantly higher than those in the CTR group. Conclusions: gut microbiota dysbiosis was induced by BFS. It was characterized by reduced LAC and increased DSV abundance and led to the deterioration of glucose/lipid metabolic dysfunction and inflammatory response, which likely promoted aorta plaque formation and the progression of atherosclerosis.
Special Issue “GPCRs: Ligands and beyond 2022”
Authors: Cione Erika , Caroleo Maria Cristina
Published: May 2022

The human genome encodes more than 800 different G protein-coupled receptors (GPCRs), uncovering their importance in human physiology [...]
Formulation and Characterization of Metformin-Loaded Ethosomes for Topical Application to Experimentally Induced Skin Cancer in Mice
Authors: Mousa Ibrahim A. , Hammady Taha M. , Gad Shadeed , Zaitone Sawsan A. , El-Sherbiny Mohamed , Sayed Ossama M.
Published: May 2022

To achieve the best treatment of skin cancer, drug penetration inside the deepest layers of the skin is an important scientific interest. We designed an ethosome formulation that serves as a carrier for metformin and measured the in vitro skin permeation. We also aimed to measure the antitumor activity of the optimal ethosomal preparation when applied topically to chemically induced skin cancer in mice. We utilized a statistical Box–Behnken experimental design and applied three variables at three levels: lecithin concentration, cholesterol concentration and a mixture of ethanol and isopropyl alcohol concentrations. All formulations were prepared to calculate the entrapment efficiency %, zeta potential, size of the vesicles and drug release % after 1, 2, 4, 8 and 24 h. The size of the vesicles for the formulations was between 124 ± 14.2 nm and 560 ± 127 nm, while the entrapment efficiency was between 97.8 ± 0.23% and 99.4 ± 0.24%, and the drug release % after 8 h was between 38 ± 0.82% and 66 ± 0.52%. All formulations were introduced into the Box–Behnken software, which selected three formulations; then, one was assigned as an optimal formula. The in vivo antitumor activity of metformin-loaded ethosomal gel on skin cancer was greater than the antitumor activity of the gel preparation containing free metformin. Lower lecithin, high ethanol and isopropyl alcohol and moderate cholesterol contents improved the permeation rate. Overall, we can conclude that metformin-loaded ethosomes are a promising remedy for treating skin cancers, and more studies are warranted to approve this activity in other animal models of skin cancers.
Low-Rate Denial-of-Service Attack Detection: Defense Strategy Based on Spectral Estimation for CV-QKD
Authors: Dai Enze , Huang Duan , Zhang Ling
Published: May 2022

Although continuous-variable quantum key distribution (CVQKD) systems have unconditional security in theory, there are still many cyber attacking strategies proposed that exploit the loopholes of hardware devices and algorithms. At present, few studies have focused on attacks using algorithm vulnerabilities. The low-rate denial-of-service (LDoS) attack is precisely an algorithm-loophole based hacking strategy, which attacks by manipulating a channel’s transmittance T. In this paper, we take advantage of the feature that the power spectral density (PSD) of LDoS attacks in low frequency band is higher than normal traffic’s to detect whether there are LDoS attacks. We put forward a detection method based on the Bartlett spectral estimation approach and discuss its feasibility from two aspects, the estimation consistency and the detection accuracy. Our experiment results demonstrate that the method can effectively detect LDoS attacks and maintain the consistency of estimation. In addition, compared with the traditional method based on the wavelet transform and Hurst index estimations, our method has higher detection accuracy and stronger pertinence. We anticipate our method may provide an insight into how to detect an LDoS attack in a CVQKD system.
Extending Focal Depth of Flower-Shaped Optical Vortex with Composited Spiral Zone Plate Grating
Authors: Hu Huakui , Cao Fulin , Zhao Yue
Published: May 2022

By combining a spiral zone plate (SZP) and a grating, we propose a single optical element, termed a composited spiral zone plate grating (CSZPG), to generate flower mode vortices with the equicohesive petals and has long focal depths. Theoretical analysis reveals that the CSZPG can generate flower mode vortices with approximately equicohesive petals, and that it has longer focal depths compared with the conventional SZP. Moreover, the performance of the CSZPG on the period, focal length and radius is investigated. The experimental results are also presented, agreeing well with the theoretical predictions. The unique characteristics of the proposed CSZPG make it attractive for many applications such as particle trapping and optical imaging.
Surface Characteristics and Color Stability of Dental PEEK Related to Water Saturation and Thermal Cycling
Authors: Porojan Liliana , Toma Flavia Roxana , Bîrdeanu Mihaela Ionela , Vasiliu Roxana Diana , Uțu Ion-Dragoș , Matichescu Anamaria
Published: May 2022

(1) Background: The study was undertaken to evaluate the surface characteristics, microhardness, and color stability of PEEK materials related to water saturation and in vitro aging. (2) Methods: Custom specimens of unmodified and modified PEEK CAD/CAM materials were investigated: BioHPP, a ceramic reinforced PEEK, and Finoframe PEEK and Juvora medical PEEK, 100% PEEK materials. Forty-eight plates were sectioned in rectangular slices. The specimens were immersed in distilled water at 37 °C for a period of 28 days, and then subjected to aging by thermal cycling (10,000 cycles). Surface roughness was measured with a contact profilometer; nanosurface topographic characterization was made by Atomic Force Microscopy; Vickers hardness measurements were performed with a micro-hardness tester; color changes were calculated. All registrations were made before immersion in water and then subsequently once a week for 4 weeks, and after thermocycling. (3) Results: The studied reinforced and unfilled PEEK materials reached water saturation after the first week of immersion, without significant differences between them. The most affected from this point of view was the reinforced PEEK material. Thermocycling induces a significant increase inmicroroughness, without significant differences between the studied materials. In relation to the nanosurface topography and roughness, the reinforced PEEK material was the least modified by aging. The color changes after 4 weeks of water immersion and one year of simulated in vitro aging ranged from extremely slight to slight, for all materials. (4) Conclusions: Water absorption was associated with a decrease in microhardness. Surface characteristics are affected by water immersion and thermocycling. Perceivable or marked color changes of the materials were not detected during the study.
Artificial Neural Network Model for the Prediction of Methane Bi-Reforming Products Using CO2 and Steam
Authors: Deng Hao , Guo Yi
Published: May 2022

The bi-reforming of methane (BRM) is a promising process which converts greenhouse gases to syngas with a flexible H2/CO ratio. As there are many factors that affect this process, the coupled effects of multi-parameters on the BRM product are investigated based on Gibbs free energy minimization. Establishing a reliable model is the foundation of process optimization. When three input parameters are changed simultaneously, the resulting BRM products are used as the dataset to train three artificial neural network (ANN) models, which aim to establish the BRM prediction model. Finally, the trained ANN models are used to predict the BRM products when the conditions vary in and beyond the training range to test their performances. Results show that increasing temperature is beneficial to the conversion of CH4. When the molar flow of H2O is at a low level, the increase in CO2 can enhance the H2 generation. While it is more than 0.200 kmol/h, increasing the CO2 flowrate leads to the increase and then decrease in the H2 molar flow in the reforming products. When the numbers of hidden layer neurons in ANN models are set as (3, 3), (3, 6) and (6, 6), all the correlation coefficients of training, validation and test are higher than 0.995. When these ANN models are used to predict the BRM products, the variation range of the prediction error becomes narrower, and the standard deviation decreases with the increase in neuron number. This demonstrates that the ANN model with more neurons has a higher accuracy. The ANN model with neuron numbers of (6, 6) can be used to predict the BRM products even when the operating conditions are beyond the training ranges, demonstrating that this model has good extension performance. This work lays the foundation for an artificial intelligent model for the BRM process, and established ANN models can be further used to optimize the operating parameters in future work.
COVID-19-Current Therapeutical Approaches and Future Perspectives
Authors: Lupașcu (Moisi) Raluca Elisabeta , Ilie Marina Ionela , Velescu Bruno Ștefan , Udeanu Denisa Ioana , Sultana Camelia , Ruță Simona , Arsene Andreea Letiția
Published: May 2022

The ongoing pandemic of coronavirus disease (COVID-19) stimulated an unprecedented international collaborative effort for rapid diagnosis, epidemiologic surveillance, clinical management, prevention, and treatment. This review focuses on the current and new therapeutical approaches, summarizing the viral structure and life cycle, with an emphasis on the specific steps that can be interfered by antivirals: (a) inhibition of viral entry with anti-spike monoclonal antibodies; (b) inhibition of the RNA genome replication with nucleosidic analogs blocking the viral RNA polymerase; (c) inhibition of the main viral protease (Mpro), which directs the formation of the nonstructural proteins. An overview of the immunomodulatory drugs currently used for severe COVID-19 treatment and future therapeutical options are also discussed.
Secularity as a Point of Reference: Specific Features of a Non-Religious and Secularized Worldview in a Family across Three Generations
Authors: Gärtner Christel
Published: May 2022

My contribution will focus on secular and non-religious worldviews and will aim to reconstruct secular relationships with the world that develop from lived values and their transmission in the family. I will try to show in detail how a non-religious habitus develops in socialization over several generations, becomes entrenched in later biographical positioning, and shapes how a person relates to the world, including their view of religion. After a brief outline of the religious field in Germany, I will concentrate on a family case whose first generation (grandparents) grew up in the GDR. This family has had no religious socialization or child baptisms for three generations and secularity has become a positive point of reference for how its members justify their own life patterns. For the members of this non-religious family, religion still becomes selectively relevant. Using concrete situations and contexts where the family has contact with religion, I will show how these encounters become a marker for drawing boundaries. In conclusion, I will follow Quack and Schuh’s distinction between “indifference to religiosity” on the one hand, and “indifference to religion” on the other, and argue that indifference to religiosity, but not indifference to religion, can be clearly identified.
Did the Islamic Stock Index Provide Shelter for Investors during the COVID-19 Crisis? Evidence from an Emerging Stock Market
Authors: Ali Kashif , Ashfaque Muhammad , Saleem Adil , Bárczi Judit , Sági Judit
Published: May 2022

The economic and financial chaos caused by COVID-19 has been a discussion topic since the beginning of 2020. This study intends to provide a parallel comparison of volatility change and external shock persistence of the Islamic and conventional stock indexes of the Pakistan Stock Exchange. The daily stock index was extracted from Eikon Thomson Reuters for the conventional and Islamic stock index from Jan 2018 to April 2021, which was further divided in three periods, i.e., full, pre-, and post-pandemic period. The data have been analyzed using generalized autoregressive conditional heteroscedasticity (GARCH). An optimally parameterized GARCH (1,1) model is used to measure volatility change for both the pre- to post-pandemic periods. The results suggest that the magnitude of risk in a conventional index is significantly higher than that of the Islamic stock index for the period of study. However, the level of COVID shock persistence is longer in the KSE (conventional) index compared to the KMI (Islamic) index.