bluuebunny/crossref_metadata_2025 huggingface dataset:
Research Regarding the Increase of Durability of Flexible Die Made from 50CrMo4 Used in the Typographic Industry
Authors: Neamțu (Folea) Gabriela Valeria , Mohora Cristina , Anania Dorel Florea , Dobrotă Dan
Published: June 2021

A large amount of packaging used mainly in the food industry is obtained by technologies that involve the use of machines such as those that cut with flexible die. The durability of the flexible die is a very important aspect and in this regard the purpose of the research was to identify technologies for its development. Thus, the research considered the analysis of the durability of the knives made of 50CrMo4 steel considering hard chrome-plating treatment, as well as laser hardening of the knives. For the analysis of the durability of the tools, two technological parameters were considered, namely the moment of the tightening force, which had values in the range of 50 Nm–110 Nm, and the die cutting speed, which was adjusted to values in the range of 50–60 m/min. For the analysis of the durability of the flexible die, the wear of the tools was taken into account, as well as the maximum length of the die cut material.
The Capacity to Secrete Insulin Is Dose-Dependent to Extremely High Glucose Concentrations: A Key Role for Adenylyl Cyclase
Authors: Gerber Katherine M. , Whitticar Nicholas B. , Rochester Daniel R. , Corbin Kathryn L. , Koch William J. , Nunemaker Craig S.
Published: June 2021

Insulin secretion is widely thought to be maximally stimulated in glucose concentrations of 16.7-to-30 mM (300-to-540 mg/dL). However, insulin secretion is seldom tested in hyperglycemia exceeding these levels despite the Guinness World Record being 147.6 mM (2656 mg/dL). We investigated how islets respond to 1-h exposure to glucose approaching this record. Insulin secretion from human islets at 12 mM glucose intervals dose-dependently increased until at least 72 mM glucose. Murine islets in 84 mM glucose secreted nearly double the insulin as in 24 mM (p < 0.001). Intracellular calcium was maximally stimulated in 24 mM glucose despite a further doubling of insulin secretion in higher glucose, implying that insulin secretion above 24 mM occurs through amplifying pathway(s). Increased osmolarity of 425-mOsm had no effect on insulin secretion (1-h exposure) or viability (48-h exposure) in murine islets. Murine islets in 24 mM glucose treated with a glucokinase activator secreted as much insulin as islets in 84 mM glucose, indicating that glycolytic capacity exists above 24 mM. Using an incretin mimetic and an adenylyl cyclase activator in 24 mM glucose enhanced insulin secretion above that observed in 84 mM glucose while adenylyl cyclase inhibitor reduced stimulatory effects. These results highlight the underestimated ability of islets to secrete insulin proportionally to extreme hyperglycemia through adenylyl cyclase activity.
A Time-Course Comparison of Skeletal Muscle Metabolomic Alterations in Walker-256 Tumour-Bearing Rats at Different Stages of Life
Authors: Chiocchetti Gabriela de Matuoka e , Lopes-Aguiar Leisa , Miyaguti Natália Angelo da Silva , Viana Lais Rosa , Salgado Carla de Moraes , Orvoën Ophelie Ocean , Florindo Derly , Santos Rogério Williams dos , Cintra Gomes-Marcondes Maria Cristina
Published: June 2021

Cancer cachexia is a severe wasting condition that needs further study to find ways to minimise the effects of damage and poor prognosis. Skeletal muscle is the most impacted tissue in cancer cachexia; thus, elucidation of its metabolic alterations could provide a direct clue for biomarker research and be applied to detect this syndrome earlier. In addition, concerning the significant changes in the host metabolism across life, this study aimed to compare the metabolic muscle changes in cachectic tumour-bearing hosts at different ages. We performed 1H-NMR metabolomics in the gastrocnemius muscle in weanling and young adult Walker-256 tumour-bearing rats at different stages of tumour evolution (initial, intermediate, and advanced). Among the 49 metabolites identified, 24 were significantly affected throughout tumour evolution and 21 were significantly affected regarding animal age. The altered metabolites were mainly related to increased amino acid levels and changed energetic metabolism in the skeletal muscle, suggesting an expressive catabolic process and diverted energy production, especially in advanced tumour stages in both groups. Moreover, these changes were more severe in weanling hosts throughout tumour evolution, suggesting the distinct impact of cancer cachexia regarding the host’s age, highlighting the need to adopting the right animal age when studying cancer cachexia.
Respiratory Parameters for the Classification of Dysfunctional Insulin Secretion by Pancreatic Islets
Authors: Kabra Uma D. , Affourtit Charles , Jastroch Martin
Published: June 2021

The development of obesity and type 2 diabetes (T2D) has been associated with impaired mitochondrial function. In pancreatic beta (β) cells, mitochondrial energy metabolism plays a central role in triggering and controlling glucose-stimulated insulin secretion (GSIS). Here, we have explored whether mitochondrial bioenergetic parameters assessed with Seahorse extracellular flux technology can quantitatively predict insulin secretion. We metabolically stressed male C57BL/6 mice by high-fat feeding (HFD) and measured the glucose sensitivity of islet respiration and insulin secretion. The diet-induced obese (DIO) mice developed hyperinsulinemia, but no pathological secretory differences were apparent between isolated DIO and chow islets. Real-time extracellular flux analysis, however, revealed a lower respiratory sensitivity to glucose in DIO islets. Correlation of insulin secretion with respiratory parameters uncovers compromised insulin secretion in DIO islets by oxidative power. Normalization to increased insulin contents during DIO improves the quantitative relation between GSIS and respiration, allowing to classify dysfunctional properties of pancreatic insulin secretion, and thereby serving as valuable biomarker for pancreatic islet glucose responsiveness and health.
Development of a Novel 2-DOF Rotary–Linear Piezoelectric Actuator Operating under Hybrid Bending–Radial Vibration Mode
Authors: Čeponis Andrius , Mažeika Dalius , Makutėnienė Daiva
Published: June 2021

The paper presents a numerical and experimental investigation of a novel two degrees of freedom (2-DOF) piezoelectric actuator that can generate rotary motion of the sphere-shaped rotor as well as induce planar motion of the flat stage. The actuator has a small size and simple design and can be integrated into a printed circuit board (PCB). The application field of the actuator is small-dimensional and high-precision positioning systems. The piezoelectric actuator comprises three rectangular bimorph plates joined with arcs and arranged by an angle of 120 degrees. A high-stiffness rod is glued on the top surface of each bimorph plate and is used to rotate the rotor or move flat stage employing contact friction force. Three U-shaped structures are used for the actuator clamping. 2-DOF rotational or planar movement is obtained by applying a harmonic or asymmetric electrical signal. The operation principle of the actuator is based on the superposition of the B20 out-of-plane bending mode of the bimorph plates and the B03 radial vibration mode of the ring. Design optimization has been performed to maximize amplitudes of contact point vibration. A prototype of the actuator was made, and a maximum rotation speed of 795.15 RPM was achieved while preload of 546.03 mN was applied. The linear velocity of 36.45 mm/s was obtained at the same preload force. Resolution measurement showed that the actuator can achieve an angular resolution of 17.48 µrad and a linear resolution of 2.75 µm.
Phylogenomic Reconstruction and Metabolic Potential of the Genus Aminobacter
Authors: Artuso Irene , Turrini Paolo , Pirolo Mattia , Lugli Gabriele Andrea , Ventura Marco , Visca Paolo
Published: June 2021

Bacteria belonging to the genus Aminobacter are metabolically versatile organisms thriving in both natural and anthropized terrestrial environments. To date, the taxonomy of this genus is poorly defined due to the unavailability of the genomic sequence of A. anthyllidis LMG 26462T and the presence of unclassified Aminobacter strains. Here, we determined the genome sequence of A. anthyllidis LMG 26462T and performed phylogenomic, average nucleotide identity and digital DNA-DNA hybridization analyses of 17 members of genus Aminobacter. Our results indicate that 16S rRNA-based phylogeny does not provide sufficient species-level discrimination, since most of the unclassified Aminobacter strains belong to valid Aminobacter species or are putative new species. Since some members of the genus Aminobacter can utilize certain C1 compounds, such as methylamines and methyl halides, a comparative genomic analysis was performed to characterize the genetic basis of some degradative/assimilative pathways in the whole genus. Our findings suggest that all Aminobacter species are heterotrophic methylotrophs able to generate the methylene tetrahydrofolate intermediate through multiple oxidative pathways of C1 compounds and convey it in the serine cycle. Moreover, all Aminobacter species carry genes implicated in the degradation of phosphonates via the C-P lyase pathway, whereas only A. anthyllidis LMG 26462T contains a symbiosis island implicated in nodulation and nitrogen fixation.
Clary Sage Cultivation and Mycorrhizal Inoculation Influence the Rhizosphere Fungal Community of an Aged Trace-Element Polluted Soil
Authors: Raveau Robin , Lounès-Hadj Sahraoui Anissa , Hijri Mohamed , Fontaine Joël
Published: June 2021

Soil fungal communities play a central role in natural systems and agroecosystems. As such, they have attracted significant research interest. However, the fungal microbiota of aromatic plants, such as clary sage (Salvia sclarea L.), remain unexplored. This is especially the case in trace element (TE)-polluted conditions and within the framework of phytomanagement approaches. The presence of high concentrations of TEs in soils can negatively affect not only microbial diversity and community composition but also plant establishment and growth. Hence, the objective of this study is to investigate the soil fungal and arbuscular mycorrhizal fungi (AMF) community composition and their changes over time in TE-polluted soils in the vicinity of a former lead smelter and under the cultivation of clary sage. We used Illumina MiSeq amplicon sequencing to evaluate the effects of in situ clary sage cultivation over two successive years, combined or not with exogenous AMF inoculation, on the rhizospheric soil and root fungal communities. We obtained 1239 and 569 fungal amplicon sequence variants (ASV), respectively, in the rhizospheric soil and roots of S. sclarea under TE-polluted conditions. Remarkably, 69 AMF species were detected at our experimental site, belonging to 12 AMF genera. Furthermore, the inoculation treatment significantly shaped the fungal communities in soil and increased the number of AMF ASVs in clary sage roots. In addition, clary sage cultivation over successive years could be one of the explanatory parameters for the inter-annual variation in both fungal and AMF communities in the soil and root biotopes. Our data provide new insights on fungal and AMF communities in the rhizospheric soil and roots of an aromatic plant, clary sage, grown in TE-polluted agricultural soil.
Comparative Genomics of Prophages Sato and Sole Expands the Genetic Diversity Found in the Genus Betatectivirus
Authors: Gillis Annika , Hock Louise , Mahillon Jacques
Published: June 2021

Tectiviruses infecting the Bacillus cereus group represent part of the bacterial “plasmid repertoire” as they behave as linear plasmids during their lysogenic cycle. Several novel tectiviruses have been recently found infecting diverse strains belonging the B. cereus lineage. Here, we report and analyze the complete genome sequences of phages Sato and Sole. The linear dsDNA genome of Sato spans 14,852 bp with 32 coding DNA sequences (CDSs), whereas the one of Sole has 14,444 bp comprising 30 CDSs. Both phage genomes contain inverted terminal repeats and no tRNAs. Genomic comparisons and phylogenetic analyses placed these two phages within the genus Betatectivirus in the family Tectiviridae. Additional comparative genomic analyses indicated that the “gene regulation-genome replication” module of phages Sato and Sole is more diverse than previously observed among other fully sequenced betatectiviruses, displaying very low sequence similarities and containing some ORFans. Interestingly, the ssDNA binding protein encoded in this genomic module in phages Sato and Sole has very little amino acid similarity with those of reference betatectiviruses. Phylogenetic analyses showed that both Sato and Sole represent novel tectivirus species, thus we propose to include them as two novel species in the genus Betatectivirus.
An Internal Promoter Drives the Expression of a Truncated Form of CCC1 Capable of Protecting Yeast from Iron Toxicity
Authors: Amaral Catarina , Vicente Cristina Teixeira , Caetano Soraia Marques , Gaspar-Cordeiro Ana , Yang Yang , Cloetens Peter , Romão Célia V. , Rodrigues-Pousada Claudina , Pimentel Catarina
Published: June 2021

In yeast, iron storage and detoxification depend on the Ccc1 transporter that mediates iron accumulation in vacuoles. While deletion of the CCC1 gene renders cells unable to survive under iron overload conditions, the deletion of its previously identified regulators only partially affects survival, indicating that the mechanisms controlling iron storage and detoxification in yeast are still far from well understood. This work reveals that CCC1 is equipped with a complex transcriptional structure comprising several regulatory regions. One of these is located inside the coding sequence of the gene and drives the expression of a short transcript encoding an N-terminally truncated protein, designated as s-Ccc1. s-Ccc1, though less efficiently than Ccc1, is able to promote metal accumulation in the vacuole, protecting cells against iron toxicity. While the expression of the s-Ccc1 appears to be repressed in the normal genomic context, our current data clearly demonstrates that it is functional and has the capacity to play a role under iron overload conditions.
Crystal Structure Evolution of CaSiO3 Polymorphs at Earth’s Mantle Pressures
Authors: Milani Sula , Comboni Davide , Lotti Paolo , Fumagalli Patrizia , Ziberna Luca , Maurice Juliette , Hanfland Michael , Merlini Marco
Published: June 2021

CaSiO3 polymorphs are abundant in only unique geological settings on the Earth’s surface and are the major Ca-bearing phases at deep mantle condition. An accurate and comprehensive study of their density and structural evolution with pressure and temperature is still lacking. Therefore, in this study we report the elastic behavior and structural evolution of wollastonite and CaSiO3-walstromite with pressure. Both minerals are characterized by first order phase transitions to denser structures. The deformations that lead to these transformations allow a volume increase ofthe bigger polyhedra, which might ease cation substitution in the structural sites of these phases. Furthermore, their geometrical features are clear analogies with those predicted and observed for tetrahedrally-structured ultra-high-pressure carbonates, which are unfortunately unquenchable. Indeed, wollastonite and CaSiO3-walstromite have a close resemblance to ultra-high-pressure chain- and ring-carbonates. This suggests a rich polymorphism also for tetrahedral carbonates, which might increase the compositional range of these phases, including continuous solid solutions involving cations with different size (Ca vs. Mg in particular) and important minor or trace elements incorporation.
Pyroxenite as a Product of Mafic-Carbonate Melt Interaction (Tazheran Massif, West Baikal Area, Russia)
Authors: Sklyarov Eugene V. , Lavrenchuk Andrey V. , Doroshkevich Anna G. , Starikova Anastasia E. , Kanakin Sergei V.
Published: June 2021

Pyroxenite and nepheline-pyroxene rocks coexist with dolomite-bearing calcite marbles in Tazheran Massif in the area of Lake Baikal, Siberia, Russia. Pyroxenites occur in a continuous elongate zone between marbles and beerbachites (metamorphosed gabbro dolerites) and in 5 cm to 20 m fragments among the marbles. Pyroxene in pyroxenite is rich in calcium and alumina (5–12 wt% Al2O3) and has a fassaite composition. The Tazheran pyroxenite may originate from a mafic subvolcanic source indicated by the presence of remnant dolerite found in one pyroxenite body. This origin can be explained in terms of interaction between mafic and crust-derived carbonatitic melts, judging by the mineralogy of pyroxenite bodies and their geological relations with marbles. According to this model, the intrusion of mantle mafic melts into thick lower crust saturated with fluids caused partial melting of silicate-carbonate material and produced carbonate and carbonate-silicate melts. The fassaite-bearing pyroxenite crystallized from a silicate-carbonate melt mixture which was produced by roughly synchronous injections of mafic, pyroxenitic, and carbonate melt batches. The ascending hydrous carbonate melts entrained fragments of pyroxenite that crystallized previously at a temperature exceeding the crystallization point of carbonates. Subsequently, while the whole magmatic system was cooling down, pyroxenite became metasomatized by circulating fluids, which led to the formation of assemblages with garnet, melilite, and scapolite.
Petrogenesis and Geodynamic Implications of Miocene Felsic Magmatic Rocks in the Wuyu Basin, Southern Gangdese Belt, Qinghai-Tibet Plateau
Authors: Chen Hanzhi , Hou Mingcai , Xiong Fuhao , Tang Hongwei , Shao Gangqiang
Published: June 2021

Miocene felsic magmatic rocks with high Sr/Y ratios are widely distributed throughout the Gangdese belt of southern Tibet. These provide a good opportunity to explore the magmatic process and deep dynamic mechanisms that occurred after collision between the Indo and the Asian plates. In this paper, felsic volcanic rocks from the Zongdangcun Formation in the Wuyu Basin in the central part of the southern Gangdese belt are used to disclose their origin. Zircon U-Pb geochronology analysis shows that the felsic magmatism occurred at ca. 10.3 ± 0.2 Ma, indicating that the Zongdangcun Formation formed during the Miocene. Most of these felsic magmatic rocks plot in the rhyolite area in the TAS diagram. The rhyolite specimens from the Zongdangcun Formation have the characteristics of high SiO2 (>64%), K2O, SiO2, and Sr contents, a low Y content and a high Sr/Y ratio, and the rocks are rich in LREE and depleted in HREE, showing geochemical affinity to adakitic rocks. The rocks have an enriched Sr-Nd isotopic composition (εNd(t) = −6.76 to −6.68, (87Sr/86Sr)i = 0.7082–0.7088), which is similar to the mixed product of the juvenile Lhasa lower continental crust and the ancient Indian crust. The Hf isotopes of zircon define a wide compositional range (εHf(t) = −4.19 to 6.72) with predominant enriched signatures. The Miocene-aged crustal thickness in southern Tibet, calculated on the basis of the Sr/Y and (La/Yb)N ratios was approximately 60–80 km, which is consistent with the thickening of the Qinghai-Tibet Plateau. The origin of Miocene felsic magmatic rocks with high Sr/Y ratios in the middle section of the Gangdese belt likely involved a partial melting of the thickened lower crust, essentially formed by the lower crust of the Lhasa block, with minor contribution from the ancient Indian crust. After comprehensively analyzing the post-collisional high Sr/Y magmatic rocks (33–8 Ma) collected from the southern margin of the Gangdese belt, we propose that the front edge tearing and segmented subduction of the Indian continental slab may be the major factor driving the east-west trending compositional changes of the Miocene adakitic rocks in southern Tibet.
Synthesis and Antiproliferative Evaluation of 2-Deoxy-N-glycosylbenzotriazoles/imidazoles
Authors: Garton Caleigh S. , DeRose Noelle K. , Dominguez Dylan , Turbi-Henderson Maria L. , Lehr Ashley L. , Padilla Ashley D. , Twining Scott D. , Casas Stephanie , Alozie Chidozie O. , Gucwa Azad L. , Elshaer Mohammed R. , De Castro Michael
Published: June 2021

A series of 2-deoxy-2-iodo-α-d-mannopyranosylbenzotriazoles was synthesized using the benzyl, 4,6-benzylidene and acetyl protected D-glucal in the presence of N-iodosuccinimide (NIS). Subsequent removal of the iodine at the C-2 position using tributyltin hydride under free radical conditions afforded the 2-deoxy-α-d-glucopyranosylbenzotriazoles in moderate to high yields. This method was extended to the preparation of substituted 2-deoxy-β-d-glucopyranosylimidazoles as well. The stereoselectivity of the addition reaction and the effect of the protecting group and temperature on anomer distribution of the benzotriazole series were also investigated. The anticancer properties of the newly synthesized compounds were evaluated in a series of viability studies using HeLa (human cervical adenocarcinoma), human breast and lung cancer cell lines. The N-[3,4,6-tri-O-benzyl-2-deoxy-α-d-glucopyranosyl]-1H-benzotriazole and the N-[3,4,6-tri-O-acetyl-2-deoxy-α-d-glucopyranosyl]-2H-benzotriazole were found to be the most potent cancer cell inhibitors at 20 µM concentrations across all four cell lines.
A Systematic Approach to Agastache mexicana Research: Biology, Agronomy, Phytochemistry, and Bioactivity
Authors: Palma-Tenango Mariana , Sánchez-Fernández Rosa E. , Soto-Hernández Marcos
Published: June 2021

Mexico is the center of origin of the species popularly known as toronjil or lemon balm (Agastache mexicana Linton & Epling). Two subspecies have been identified and are commonly called purple or red (Agastache mexicana Linton & Epling subspecies. mexicana) and white (Agastache mexicana subspecies xolocotziana Bye, E.L. Linares & Ramamoorthy). Plants from these subspecies differ in the size and form of inflorescence and leaves. They also possess differences in their chemical compositions, including volatile compounds. Traditional Mexican medicine employs both subspecies. A. mexicana exhibits a broad range of pharmacological properties, such as anti-inflammatory, anxiolytic, and antioxidant. A systematic vision of these plant’s properties is discussed in this review, exposing its significant potential as a source of valuable bioactive compounds. Furthermore, this review provides an understanding of the elements that make up the species’ holistic system to benefit from lemon balm sustainably.
Immunomodulatory Effects of Green Tea Polyphenols
Authors: Wang Shuzhen , Li Zhiliang , Ma Yuting , Liu Yan , Lin Chi-Chen , Li Shiming , Zhan Jianfeng , Ho Chi-Tang
Published: June 2021

Green tea and its bioactive components, especially polyphenols, possess many health-promoting and disease-preventing benefits, especially anti-inflammatory, antioxidant, anticancer, and metabolic modulation effects with multi-target modes of action. However, the effect of tea polyphenols on immune function has not been well studied. Moreover, the underlying cellular and molecular mechanisms mediating immunoregulation are not well understood. This review summarizes the recent studies on the immune-potentiating effects and corresponding mechanisms of tea polyphenols, especially the main components of (–)-epigallocatechin-3-gallate (EGCG) and (–)-epicatechin-3-gallate (ECG). In addition, the benefits towards immune-related diseases, such as autoimmune diseases, cutaneous-related immune diseases, and obesity-related immune diseases, have been discussed.
The Fumarprotocetraric Acid Inhibits Tau Covalently, Avoiding Cytotoxicity of Aggregates in Cells
Authors: González Camila , Cartagena Constanza , Caballero Leonardo , Melo Francisco , Areche Carlos , Cornejo Alberto
Published: June 2021

Neurodegenerative disorders, including Tauopathies that involve tau protein, base their pathological mechanism on forming proteinaceous aggregates, which has a deleterious effect on cells triggering an inflammatory response. Moreover, tau inhibitors can exert their mechanism of action through noncovalent and covalent interactions. Thus, Michael’s addition appears as a feasible type of interaction involving an α, β unsaturated carbonyl moiety to avoid pathological confirmation and further cytotoxicity. Moreover, we isolated three compounds from Antarctic lichens Cladonia cariosa and Himantormia lugubris: protolichesterinic acid (1), fumarprotocetraric acid (2), and lichesterinic acid (3). The maleimide cysteine labeling assay showed that compounds 1, 2, and 3 inhibit at 50 µM, but compounds 2 and 3 are statistically significant. Based on its inhibition capacity, we decided to test compound 2 further. Thus, our results suggest that compound 2 remodel soluble oligomers and diminish β sheet content, as demonstrated through ThT experiments. Hence, we added externally treated oligomers with compound 2 to demonstrate that they are harmless in cell culture. First, the morphology of cells in the presence of aggregates does not suffer evident changes compared to the control. Additionally, the externally added aggregates do not provoke a substantial LDH release compared to the control, indicating that treated oligomers do not provoke membrane damage in cell culture compared with aggregates alone. Thus, in the present work, we demonstrated that Michael’s acceptors found in lichens could serve as a scaffold to explore different mechanisms of action to turn tau aggregates into harmless species.
NMR of Natural Products as Potential Drugs
Authors: Hansen Poul Erik
Published: June 2021

This review outlines methods to investigate the structure of natural products with emphasis on intramolecular hydrogen bonding, tautomerism and ionic structures using NMR techniques. The focus is on 1H chemical shifts, isotope effects on chemical shifts and diffusion ordered spectroscopy. In addition, density functional theory calculations are performed to support NMR results. The review demonstrates how hydrogen bonding may lead to specific structures and how chemical equilibria, as well as tautomeric equilibria and ionic structures, can be detected. All these features are important for biological activity and a prerequisite for correct docking experiments and future use as drugs.
Hydration Kinetics for Alkaline Activation of Slag from Color Variation Data
Authors: Ding Zhaoyang , Zhou Jinghai , Su Qun , Sun Hong , Zhang Yichao , Wang Qing , Bian Hongguang , Dong Fengxin
Published: June 2021

In this study, we explore a new method based on color variation data to derive the kinetics of the entire process of the hydration of alkali-activated slag (AAS). Using this image analysis technique, we can monitor the induction period that cannot be observed using conventional microcalorimetry techniques. Color variation was recorded across a sequence of 9999 images, which were processed via MATLAB software package. Further, an average pixel value (APV) was determined to represent the color in each image. Reaction parameters, such as color variation velocity v(t), reaction speed ε(t), and hydration degree α(t), that govern the entire hydration process were determined. On the basis of the reaction parameters and a Krstulovic–Dabic kinetic model, integral and differential equations were derived to simulate the three basic processes of AAS hydration. Equations describing the reaction kinetics of AAS with solutions of three different concentrations of NaOH were extracted using this method.
Presence of Cholesterol in Non-Animal Organisms: Identification and Quantification of Cholesterol in Crude Seed Oil from Perilla frutescens and Dehydrated Pyropia tenera
Authors: Oh Min-Ji , So Hee-Jin , Hong Eun-Sik , Shin Jung-Ah , Lee Ki-Teak
Published: June 2021

Studies have reported that cholesterol, a molecule found mainly in animals, is also present in some plants and algae. This study aimed to determine whether cholesterol exists in three dehydrated algae species, namely, Pyropia tenera, Saccharina japonica, and Undaria pinnatifida, and in one plant species, namely, Perilla frutescens (four perilla seed oil samples were analyzed). These species were chosen for investigation because they are common ingredients in East Asian cuisine. Gas chromatography-flame ionization detection (GC-FID) analysis found that cholesterol was present in P. tenera (14.6 mg/100 g) and in all four perilla seed oil samples (0.3–0.5 mg/100 g). High-performance liquid chromatography with evaporative light-scattering detection (HPLC-ELSD) also demonstrated that cholesterol was present in P. tenera (14.2 mg/100 g) and allowed the separation of cholesterol from its isomer lathosterol. However, cholesterol could not be detected by HPLC-ELSD in the perilla seed oil samples, most likely because it is only present in trace amounts. Moreover, liquid chromatography-tandem mass spectrometry (LC-MS/MS) confirmed the presence of cholesterol in both P. tenera and perilla seed oil. MRM results further suggested that lathosterol (a precursor of cholesterol) was present in P. tenera.
25th Anniversary of Molecules—Recent Advances in Green Chemistry
Authors: de Gonzalo Gonzalo , Freire Mara G.
Published: June 2021

Green Chemistry has been defined by the EPA as the design of chemical products and processes that reduce or eliminate the use or generation of hazardous substances [...]
Formulation Improvements in the Applications of Surfactant–Oil–Water Systems Using the HLDN Approach with Extended Surfactant Structure
Authors: Forgiarini Ana M. , Marquez Ronald , Salager Jean-Louis
Published: June 2021

Soap applications for cleaning and personal care have been used for more than 4000 years, dating back to the pharaonic period, and have widely proliferated with the appearance of synthetic surfactants a century ago. Synthetic surfactants used to make macro-micro-nano-emulsions and foams are used in laundry and detergency, cosmetics and pharmaceuticals, food conditioning, emulsified paints, explosives, enhanced oil recovery, wastewater treatment, etc. The introduction of a multivariable approach such as the normalized hydrophilic–lipophilic deviation (HLD N) and of specific structures, tailored with an intramolecular extension to increase solubilization (the so-called extended surfactants), makes it possible to improve the results and performance in surfactant–oil–water systems and their applications. This article aims to present an up-to-date overview of extended surfactants. We first present an introduction regarding physicochemical formulation and its relationship with performance. The second part deals with the importance of HLD N to make a straightforward classification according to the type of surfactants and how formulation parameters can be used to understand the need for an extension of the molecule reach into the oil and water phases. Then, extended surfactant characteristics and strategies to increase performance are outlined. Finally, two specific applications, i.e., drilling fluids and crude oil dewatering, are described.
Fluid Intake Monitoring Systems for the Elderly: A Review of the Literature
Authors: Cohen Rachel , Fernie Geoff , Roshan Fekr Atena
Published: June 2021

Fluid intake monitoring is an essential component in preventing dehydration and overhydration, especially for the senior population. Numerous critical health problems are associated with poor or excessive drinking such as swelling of the brain and heart failure. Real-time systems for monitoring fluid intake will not only measure the exact amount consumed by the users, but could also motivate people to maintain a healthy lifestyle by providing feedback to encourage them to hydrate regularly throughout the day. This paper reviews the most recent solutions to automatic fluid intake monitoring both commercially and in the literature. The available technologies are divided into four categories: wearables, surfaces with embedded sensors, vision- and environmental-based solutions, and smart containers. A detailed performance evaluation was carried out considering detection accuracy, usability and availability. It was observed that the most promising results came from studies that used data fusion from multiple technologies, compared to using an individual technology. The areas that need further research and the challenges for each category are discussed in detail.
Predictors of COVID-19-Related Perceived Improvements in Dietary Health: Results from a US Cross-Sectional Study
Authors: Cosgrove Kelly , Wharton Christopher
Published: June 2021

The COVID-19 pandemic resulted in substantial lifestyle changes. No US study has identified predictors of perceived dietary healthfulness changes during the pandemic period. This study included analyses of lifestyle and dietary healthfulness changes using 958 survey responses from US primary household food purchasers. Information was collected related to demographics, COVID-19-related household changes, and health-related habits before and during the pandemic. Binary logistic regression identified predictors of perceived increase in dietary healthfulness during the pandemic period. Overall, 59.8%, 16.4%, and 23.4% of participants reported that their eating habits likely changed, may have changed, and likely did not change, respectively. Of the participants whose dietary habits likely or may have changed, 64.1%, 16.8%, and 19% reported healthier, neither healthier nor less healthy, and less healthy eating habits, respectively. COVID-19-related income loss, more meals consumed with household members in front of the television, an increase in food advertisement exposure, increased perceived stress, and better perceived current health were significant predictors of a perceived increase in dietary healthfulness. Overall, dietary habits were perceived to become healthier during the pandemic. The predictors of perceived improvement in dietary healthfulness were surprising and indicate the need for further study of these factors in crisis and noncrisis situations.
Regulation of Neurotransmitters by the Gut Microbiota and Effects on Cognition in Neurological Disorders
Authors: Chen Yijing , Xu Jinying , Chen Yu
Published: June 2021

Emerging evidence indicates that gut microbiota is important in the regulation of brain activity and cognitive functions. Microbes mediate communication among the metabolic, peripheral immune, and central nervous systems via the microbiota–gut–brain axis. However, it is not well understood how the gut microbiome and neurons in the brain mutually interact or how these interactions affect normal brain functioning and cognition. We summarize the mechanisms whereby the gut microbiota regulate the production, transportation, and functioning of neurotransmitters. We also discuss how microbiome dysbiosis affects cognitive function, especially in neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease.
The Antioxidant Effects of Whey Protein Peptide on Learning and Memory Improvement in Aging Mice Models
Authors: Yu Xiao-Chen , Li Zhen , Liu Xin-Ran , Hu Jia-Ni , Liu Rui , Zhu Na , Li Yong
Published: June 2021

This study investigated the antioxidant effects of whey protein peptide on learning and memory in aging C57BL/6N mice. A total of 72 SPF male C57BL/6N mice were used. Twelve mice were randomly selected as the control group, and the other mice were intraperitoneally injected with D-galactose (100 mg/kg body weight for 6 weeks), during which, the mice in the control group were intraperitoneally injected with the same amount of normal saline. After 6 weeks, the blood was taken from the epicanthus and the serum MDA level was measured, according to which, the mice were randomly divided into the model control group, the whey protein group (1.5 g/kg body weight), and three Whey protein peptide (WHP) intervention groups (0.3 g/kg body weight, 1.5 g/kg body weight, 3.0 g/kg body weight). The water solution of the test sample was administered by oral gavage every day. The intervention period was 30 days, during which, the model control group, the whey protein group, and the whey protein peptide group continued receiving intraperitoneal injections of D-galactose, while the control group continued receiving intraperitoneal injections of normal saline. After the intervention, behavioral experiments were conducted in the following order: open field test, water maze test, and new object recognition test. After the behavioral experiment, the morphology of hippocampal formation was observed by HE staining and TUNEL labeling. Oxidative stress-related indexes in the serum, liver, and brain were detected. Expression levels of the cholinergic system-related enzymes and proinflammatory cytokines in brain tissue were detected. Western blot was used to detect the expression of synaptic plasticity-related proteins in the mouse brain. The results showed that WHP could significantly improve the accumulation of MDA and PC, increase the activities of SOD and GSH-Px, resist oxidative stress injury, and enhance the potential of endogenous antioxidant defense mechanisms. WHP can significantly improve the decline of aging-related spatial exploration, body movement, and spatial and non-spatial learning/memory ability. Its specific mechanism may be related to reducing the degeneration of hippocampal nerve cells, reducing the apoptosis of nerve cells, improving the activity of AChE, reducing the expression of inflammatory factors (TNF-α and IL-1β) in brain tissue, reducing oxidative stress injury, and improving the expression of p-CaMKⅡ and BDNF synaptic plasticity protein. These results indicate that WHP can improve aging-related oxidative stress, as well as learning and memory impairment.
Applying the Stages of Change Model in a Nutrition Education Programme for the Promotion of Fruit and Vegetable Consumption among People with Severe Mental Disorders (DIETMENT)
Authors: Vilamala-Orra Mireia , Vaqué-Crusellas Cristina , Foguet-Boreu Quintí , Guimerà Gallent Marta , del Río Sáez Ruben
Published: June 2021

Despite growing evidence of the benefits of adequate intake of fruit and vegetables (F&V) and the recommendation to consume five servings daily, the adoption of these habits is poor among people with severe mental disorder (SMD). The main aim of the present study is to determine changes in the intake of F&V and motivation to do so among people with SMDs after participating in a food education programme. A community-based randomized controlled trial was conducted in Spain, with the intervention group (IG) participating in a food education programme based on the stages of change model to promote consumption of F&V and the control group (CG) receiving three informative sessions on basic healthy eating. The main outcomes were related to the intake of F&V and stages of change. Data collection was performed at baseline, post intervention, and 12-month follow-up. Seventy-four participants enrolled in the study and sixty completed the 12-month follow-up. An increase in motivation towards the intake of F&V was observed in the IG but not in the CG (McNemar’s test p = 0.016, p = 0.625). No significant difference was observed for the intake of fruit, vegetables, or F&V. Basing food education strategies on the stages of change model shows positive results, increasing the awareness and disposition of people with SMD towards the intake of F&V. More research is needed to identify the most appropriate eating intervention to increase the intake of F&V.
The Role of High Triglycerides Level in Predicting Cognitive Impairment: A Review of Current Evidence
Authors: Dimache Alina Mihaela , Șalaru Delia Lidia , Sascău Radu , Stătescu Cristian
Published: June 2021

The burden of cognitive disorders is huge and still growing, however the etiology and the degree of cognitive impairment vary considerably. Neurodegenerative and vascular mechanisms were most frequently assessed in patients with dementia. Recent studies have shown the possible involvement of triglycerides levels in cognitive function through putative mechanisms such as brain blood barrier dysfunction or amyloid metabolism imbalance, but not all research in the field found this association. Several clinical studies evaluated the relationship between different forms of cognitive decline and levels of serum triglycerides, independent of other cardiovascular risk factors. This review focuses on the role of triglycerides in cognitive decline, cerebral amyloidosis and vascular impairment. Considering that the management of hypertriglyceridemia benefits from lifestyle modification, diet, and specific drug therapy, future studies are requested to appraise the triglycerides–cognitive impairment relationship.
De-Coding the Contributions of the Viral RNAs to Alphaviral Pathogenesis
Authors: LaPointe Autumn T. , Sokoloski Kevin J.
Published: June 2021

Alphaviruses are positive-sense RNA arboviruses that are capable of causing severe disease in otherwise healthy individuals. There are many aspects of viral infection that determine pathogenesis and major efforts regarding the identification and characterization of virulence determinants have largely focused on the roles of the nonstructural and structural proteins. Nonetheless, the viral RNAs of the alphaviruses themselves play important roles in regard to virulence and pathogenesis. In particular, many sequences and secondary structures within the viral RNAs play an important part in the development of disease and may be considered important determinants of virulence. In this review article, we summarize the known RNA-based virulence traits and host:RNA interactions that influence alphaviral pathogenesis for each of the viral RNA species produced during infection. Overall, the viral RNAs produced during infection are important contributors to alphaviral pathogenesis and more research is needed to fully understand how each RNA species impacts the host response to infection as well as the development of disease.
Mesenchymal Stem Cells Engineered by Nonviral Vectors: A Powerful Tool in Cancer Gene Therapy
Authors: Ding Yuan , Wang Chenyang , Sun Zhongquan , Wu Yingsheng , You Wanlu , Mao Zhengwei , Wang Weilin
Published: June 2021

Due to their “tumor homing” and “immune privilege” characteristics, the use of mesenchymal stem cells (MSCs) has been proposed as a novel tool against cancer. MSCs are genetically engineered in vitro and then utilized to deliver tumoricidal agents, including prodrugs and bioactive molecules, to tumors. The genetic modification of MSCs can be achieved by various vectors, and in most cases viral vectors are used; however, viruses may be associated with carcinogenesis and immunogenicity, restricting their clinical translational potential. As such, nonviral vectors have emerged as a potential solution to address these limitations and have gradually attracted increasing attention. In this review, we briefly revisit the current knowledge about MSC-based cancer gene therapy. Then, we summarize the advantages and challenges of nonviral vectors for MSC transfection. Finally, we discuss recent advances in the development of new nonviral vectors, which have provided promising strategies to overcome obstacles in the gene modulation of MSCs.
Electrospun Membranes as a Porous Barrier for Molecular Transport: Membrane Characterization and Release Assessment
Authors: Liu Weiyi , Walker Greg , Price Sally , Yang Xiangdong , Li Juan , Bunt Craig
Published: June 2021

Electrospun nanofibers have been extensively studied for encapsulated drugs releasing from the inside of the fiber matrix, but have been barely looked at for their potential to control release as a semi-permeable membrane. This study investigated molecular transport behaviors across nanofiber membranes with different micro-structure sizes and compositions. Four types of membranes were made by 5% and 10% poly (ε-caprolactone) (PCL) solutions electro-spun with or without 50 nm calcium carbonate (CaCO3) nanoparticles. The membranes were tested for thickness, fiber diameter, pore size, porosity, tensile strength and elongation, contact angle of water and their impacts on molecular transport behaviors. The presence of the CaCO3 nanoparticles made the 5% membranes stronger and stiffer but the 10% membranes weaker and less stiff due to the different (covering or embedded) locations of the nanoparticles with the corresponding fibers. Solute transport studies using caffeine as the model drug found the 5% membranes further retarded release from the 10% membranes, regardless of only half the amount of material being used for synthesis. The addition of CaCO3 nanoparticles aided the water permeation process and accelerated initial transports. The difference in release profiles between 5% and 10% membranes suggests different release mechanisms, with membrane-permeability dominated release for 5% PCL membranes and solute-concentration-gradient dominated release for 10% PCL membranes.
Imaging-Based Characterization of a Slco2b1(-/-) Mouse Model Using [11C]Erlotinib and [99mTc]Mebrofenin as Probe Substrates
Authors: Marie Solène , Hernández-Lozano Irene , Breuil Louise , Truillet Charles , Hu Shuiying , Sparreboom Alex , Tournier Nicolas , Langer Oliver
Published: June 2021

Organic anion-transporting polypeptide 2B1 (OATP2B1) is co-localized with OATP1B1 and OATP1B3 in the basolateral hepatocyte membrane, where it is thought to contribute to the hepatic uptake of drugs. We characterized a novel Slco2b1(-/-) mouse model using positron emission tomography (PET) imaging with [11C]erlotinib (a putative OATP2B1-selective substrate) and planar scintigraphic imaging with [99mTc]mebrofenin (an OATP1B1/1B3 substrate, which is not transported by OATP2B1). Dynamic 40-min scans were performed after intravenous injection of either [11C]erlotinib or [99mTc]mebrofenin in wild-type and Slco2b1(-/-) mice. A pharmacokinetic model was used to estimate the hepatic uptake clearance (CL1) and the rate constants for transfer of radioactivity from the liver to the blood (k2) and excreted bile (k3). CL1 was significantly reduced in Slco2b1(-/-) mice for both radiotracers (p < 0.05), and k2 was significantly lower (p < 0.01) in Slco2b1(-/-) mice for [11C]erlotinib, but not for [99mTc]mebrofenin. Our data support previous evidence that OATP transporters may contribute to the hepatic uptake of [11C]erlotinib. However, the decreased hepatic uptake of the OATP1B1/1B3 substrate [99mTc]mebrofenin in Slco2b1(-/-) mice questions the utility of this mouse model to assess the relative contribution of OATP2B1 to the liver uptake of drugs which are substrates of multiple OATPs.
Efficient and Noise Robust Photon-Counting Imaging with First Signal Photon Unit Method
Authors: Hua Kangjian , Liu Bo , Chen Zhen , Fang Liang , Wang Huachuang
Published: June 2021

Efficient photon-counting imaging in low signal photon level is challenging, especially when noise is intensive. In this paper, we report a first signal photon unit (FSPU) method to rapidly reconstruct depth image from sparse signal photon counts with strong noise robustness. The method consists of acquisition strategy and reconstruction strategy. Different statistic properties of signal and noise are exploited to quickly distinguish signal unit during acquisition. Three steps, including maximum likelihood estimation (MLE), anomaly censorship and total variation (TV) regularization, are implemented to recover high quality images. Simulations demonstrate that the method performs much better than traditional photon-counting methods such as peak and cross-correlation methods, and it also has better performance than the state-of-the-art unmixing method. In addition, it could reconstruct much clearer images than the first photon imaging (FPI) method when noise is severe. An experiment with our photon-counting LIDAR system was conducted, which indicates that our method has advantages in sparse photon-counting imaging application, especially when signal to noise ratio (SNR) is low. Without the knowledge of noise distribution, our method reconstructed the clearest depth image which has the least mean square error (MSE) as 0.011, even when SNR is as low as −10.85 dB.