bluuebunny/crossref_metadata_2025 huggingface dataset:
What bothers severe asthma patients most? A paired patient–clinician study across seven European countries
Authors: Ainsworth Ben , Chatburn Eleanor , Bansal Aruna T. , Fulton Olivia , Hamerlijnck Dominique , Coleman Courtney , Eger Katrien , Hyland Michael , Holmes Joshua , Heaney Liam , Sedlák Vratislav , Škrgat Sabina , Edelbaher Natalija , ten Brinke Anneke , Porsbjerg Celeste , Gaga Mina , Loureiro Claudia , Djukanovic Ratko , Berret Emmanuelle , Kwon Namhee
Published: March 2023

Introduction

Severe asthma is a complex, multidimensional disease. Optimal treatment, adherence and outcomes require shared decision-making, rooted in mutual understanding between patient and clinician. This study used a novel, patient-centred approach to examine the most bothersome aspects of severe asthma to patients, as seen from both perspectives in asthma registries.

Methods

Across seven countries, 126 patients with severe asthma completed an open-ended survey regarding most the bothersome aspect(s) of their asthma. Patients’ responses were linked with their treating clinician who also completed a free-text survey about each patient's most bothersome aspect(s). Responses were coded using content analysis, and patient and clinician responses were compared. Finally, asthma registries that are part of the SHARP (Severe Heterogeneous Asthma Research collaboration, Patient-centred) Clinical Research Collaboration were examined to see the extent to which they reflected the most bothersome aspects reported by patients.

Results

88 codes and 10 themes were identified. Clinicians were more focused on direct physical symptoms and were less focused on “holistic” aspects such as the effort required to self-manage the disease. Clinicians accurately identified a most bothersome symptom for 29% of patients. Agreement was particularly low with younger patients and those using oral corticosteroids infrequently. In asthma registries, patient aspects were predominantly represented in questionnaires.

Conclusions

Results demonstrated different perspectives and priorities between patients and clinicians, with clinicians more focused on physical aspects. These differences must be considered when treating individual patients, and within multidisciplinary treatment teams. The use of questionnaires that include multifaceted aspects of disease may result in improved asthma research.
Homocysteine levels correlate with AVSS-RigiScan test parameters in men with erectile dysfunction
Authors: Qian Xin , Tao Xing , Gong Yangyang , Ran Can , Feng Yougang , Liu Hongjian
Published: March 2023

Background

Although elevated homocysteine levels have been shown to affect penile erection, the relationship between homocysteine and erection at the tip or base of the penis has not been extensively studied.

Results

We found that homocysteine levels were negatively correlated with the average event rigidity of the base (r = -0.2225, p = 0.0142). Homocysteine levels were also negatively correlated with the average maximum rigidity of the base (r = -0.2164, p = 0.0171). In particular, homocysteine levels were negatively correlated with ∆ Tumescence of the tip (r = -0.1866, p = 0.0404). Similarly, homocysteine was negatively correlated with ∆ Tumescence of the base (r = -0.2257, p = 0.0128).

Conclusion

Our data showed that homocysteine inhibits penile erection. At the same time, homocysteine levels were negatively correlated with the parameters of the AVSS-RigiScan test.
Study of the error correction capability of multiple sequence alignment algorithm (MAFFT) in DNA storage
Authors: Xie Ranze , Zan Xiangzhen , Chu Ling , Su Yanqing , Xu Peng , Liu Wenbin
Published: March 2023

Synchronization (insertions–deletions) errors are still a major challenge for reliable information retrieval in DNA storage. Unlike traditional error correction codes (ECC) that add redundancy in the stored information, multiple sequence alignment (MSA) solves this problem by searching the conserved subsequences. In this paper, we conduct a comprehensive simulation study on the error correction capability of a typical MSA algorithm, MAFFT. Our results reveal that its capability exhibits a phase transition when there are around 20% errors. Below this critical value, increasing sequencing depth can eventually allow it to approach complete recovery. Otherwise, its performance plateaus at some poor levels. Given a reasonable sequencing depth (≤ 70), MSA could achieve complete recovery in the low error regime, and effectively correct 90% of the errors in the medium error regime. In addition, MSA is robust to imperfect clustering. It could also be combined with other means such as ECC, repeated markers, or any other code constraints. Furthermore, by selecting an appropriate sequencing depth, this strategy could achieve an optimal trade-off between cost and reading speed. MSA could be a competitive alternative for future DNA storage.
Associations of sleeping, sedentary and physical activity with phenotypic age acceleration: a cross-sectional isotemporal substitution model
Authors: Han Mengying , Fang Jiaxin , Zhang Yixin , Song Xingxu , Jin Lina , Ma Yanan
Published: March 2023

Background

Physical activity was believed to be associated with reduced aging among adults, while the competing nature of the physical activity and sedentary behavior has mainly been neglected in studies. We aimed to estimate the association of sleeping, sedentary behavior, and physical activity with aging among adults, considering the competing nature between variables of activity status.

Methods

A total of 5288 participants who were 20 years or older from the National Health and Nutrition Examination Survey were involved. The questionnaire was used to collect data regarding sociodemographics (age, sex, ethnicity/race, and education), and lifestyle behaviors (smoking, drinking). The Global Physical Activity Questionnaire was used to measure self-reported time for sedentary behavior, walking/bicycling, and moderate-to-vigorous physical activity (MVPA). The sleeping duration was obtained via interview. Phenotypic age acceleration (PhenoAgeAccel) was calculated as an aging index using nine chemistry biomarkers. Isotemporal substitution models using multivariable linear regression to examine the associations of sleeping, sedentary behavior, and physical activity with PhenoAgeAccel, stratified by MVPA (< 150 min/week, ≥ 150 min/week).

Results

Thirty minutes per day spent on sedentary behavior was positively associated with PhenoAgeAccel (β = 0.07, 95% CI: 0.04, 0.11), and 30 min/day spent on leisure-time MVPA was adversely associated with PhenoAgeAccel (β =  − 0.55, 95% CI: − 0.73, − 0.38). Replacing 30 min/day sedentary behaviors with 30 min/day of MVPA (β = -3.98, 95% CI: -6.22, -1.74) or 30 min/day of walking/bicycling (β = -0.89, 95% CI: -1.10, -0.68) was adversely associated with PhenoAgeAccel. Substituting 30 min/day of walking/bicycling for 30 min/day of leisure-time MVPA was positively associated with PhenoAgeAccel (β = 3.09, 95% CI: 0.93, 5.25).

Conclusion

Sedentary behavior was positively associated with aging. Replacing sedentary behaviors with walking/bicycling or MVPA was adversely associated with aging among adults.
Brief and long co-incubation of sperm and oocytes for in vitro fertilization: a meta-analysis of randomized controlled trials
Authors: Fan Yiyue , Wu Zeyu , Peng Fang , Peng Hongyao , Liang Xin , Zhu Shaomi
Published: March 2023

Background

There is still no consensus on the optimal time of oocyte–sperm co-incubation during in vitro fertilization and embryo transfer (IVF-ET). The aim of this meta-analysis was to compare the effects of brief (1-6 h) and long (16-24 h) gametes co-incubation time on IVF outcomes.

Methods

The study protocol was registered online through PROSPERO (CRD42022337503) and PRISMA guidelines were followed in the present study. The following databases were searched from inception to May 2022 for randomized controlled trials (RCTs): PubMed, Embase, Cochrane library, Web of Science, using search terms related to IVF, gametes, time of co-incubation and reproductive outcome measure. Studies comparing outcomes of brief co-incubation to that of long co-incubation during IVF, and reporting primary outcome (live birth rate), secondary outcomes (clinical pregnancy rate; ongoing pregnancy rate; miscarriage rate; normal fertilization rate; polyspermy rate; top-quality embryo rate; implantation rate) were searched. A total of 11 studies were included in the meta-analysis. Combined odds ratio (OR) and 95% confidence interval (CI) were calculated for the data. Statistical heterogeneity analysis between studies was assessed by Cochran Q and I2 statistic with a significant threshold of P < 0.05. Methodologic quality assessment of RCTs was made for potential risk of bias with Cochrane Risk of Bias Tool.

Results

Compared to long-term co-incubation, brief co-incubation had an advantage in increasing implantation rate (OR: 1.97, 95% CI: 1.52–2.57), ongoing pregnancy rate (OR: 2.18, 95% CI: 1.44–3.29) and top-quality embryo rate (OR: 1.17, 95% CI: 1.02–1.35). However, brief co-incubation of gametes had no advantages in the live-birth rate (OR: 1.09, 95% CI: 0.72–1.65), miscarriage rate (OR: 1.32, 95% CI: 0.55–3.18), clinical pregnancy rate (OR: 1.36, 95% CI: 0.99–1.87) and polyspermy rate (OR: 0.80, 95% CI: 0.48–1.33) than long-term co-incubation. Additionally, the brief co-incubation was associated with lower normal fertilization rate (OR: 0.89, 95% CI: 0.80–0.99), compared with long co-incubation.

Conclusions

Brief co-incubation of gametes had the advantages in increasing implantation rate, ongoing pregnancy rate and top-quality embryo rate than long-term co-incubation. However, the live-birth rate displayed no difference between the two in vitro fertilization methods. Gametes co-incubation time should be individualized according to each patient’s IVF history, infertility causes and the semen parameters.
Lifestyle and the hypertensive disorders of pregnancy in nulliparous women in the United States: a secondary data analysis of the nuMom2b
Authors: Mollard Elizabeth , Cottrell Constance
Published: March 2023

Background

Hypertensive disorders of pregnancy are a leading cause of maternal and fetal morbidity and mortality and a significant risk factor for future cardiovascular disease development in women. This study aimed to explore lifestyle wellness-related variables and how they impact the risk of hypertension in pregnancy.

Methods

This is a secondary analysis of data from the prospective cohort study Nulliparous Pregnancy Outcomes Study: Monitoring Mothers-To-Be (nuMoM2b). Data was collected through questionnaires, clinical evaluations, and medical records review at 8 academic medical centers in the United States. Four study visits were scheduled throughout the participant’s pregnancy (visits one–four): 60–136, 160–216, and 220–296 weeks gestation and birth. A series of statistical modeling and logistical regression were performed using 15 lifestyle variables related to sleep, nutrition, resilience, illness avoidance, and physical activity were selected as predictor variables with an outcome variable of hypertension.

Results

Of 9289 nulliparous participants considered for inclusion in our analyses, 1464 had any HDP during study participation, and 554 participants had complete data available for the study and were included in our final sample. Results were statistically significant at a level of p < 0.05. Of the sleep variables, snoring at visit 1 increased the risk of hypertension in pregnancy. Greater vegetable consumption reported at visit one decreased risks of hypertension in pregnancy. Physical activity reported at visit two and visit three were associated with decreased risk of hypertension. Physical activity reported at visit three combined with more hours of sleep each night, or through napping habit reported at visit one decreased hypertension risk. Increased fish oil consumption combined with more hours of sleep at visit one increased odds of hypertension in pregnancy.

Conclusions

Our results support that lifestyle wellness-related variables relating to sleep, physical activity and nutrition affect hypertension in pregnancy. The studied variables and others should be considered in future research and intervention development to reduce hypertension in pregnancy and improve maternal wellness.
Influence of clusterin genetic variants on IOP elevation in pseudoexfoliation syndrome and pseudoexfoliative glaucoma in Turkish population
Authors: Demirdöğen Birsen Can , Demirkaya-Budak Sinem
Published: March 2023

Purpose

Pseudoexfoliation syndrome (PEX) is distinguished by the deposition of fibrillary material within the aqueous humor and, in most cases, causes pseudoexfoliative glaucoma (PEG). The pathophysiologies of PEX and PEG are not completely explained. Therefore, this study aimed to assess the potential relationship between single nucleotide polymorphisms (SNPs) in the 3ʹ untranslated region or introns of the clusterin gene (CLU) and the susceptibility to developing PEG or PEX.

Methods

Two hundred and forty patients with PEX, 239 patients with PEG, and 240 control subjects were included. Genotyping was carried out using real-time PCR (rs2279590 C/T and rs1532278 C/T) or PCR followed by restriction endonuclease digestion (rs11136000 C/T and rs3087554 T/C).

Results

The minor alleles or genotypes of CLU SNPs were not significantly associated with PEX or PEG. IOP values of patients with PEX carrying the homozygote polymorphic TT genotype were significantly elevated compared with PEX cases with the CT or CC genotypes for rs2279590, rs11136000 and rs1532278 (P = .009, P = .007, P = .010, respectively).

Conclusion

We present the first evidence that three SNPs in CLU gene (rs2279590, rs11136000 and rs1532278) might induce a rise in IOP in patients with PEX, conferring susceptibility to develop PEG.
Telecommuting-related health outcomes during the COVID-19 pandemic in South Korea: a national population-based cross-sectional study
Authors: Ryoo Seung-Woo , Min Jin-Young , Lee Dong-Wook , Choi Baek-Yong , Choi Juho , Kim Ho-Yeon , Min Kyoung-Bok
Published: March 2023

Background

Telecommuting has expanded greatly during the COVID-19 pandemic. Since the advent of remote working from home, there has been an ongoing controversy about the positive or negative health-related impact of telecommuting. This study aimed to investigate change in the occupational health risk in South Korean workers involved in telecommuting during the pandemic period compared to daily commuters.

Methods

A population-based cross-sectional study of South Korean workers using the secondary data from the 6th Korean Working Conditions Survey (2020–2021) was designed. A total of 12,354 white-collar wage employees were selected as the study sample. Telecommuting, depression, anxiety, insomnia, fatigue, musculoskeletal pain, headache-eye strain, absenteeism, and presenteeism were measured by self-reported data. Multiple logistic regression models, including gender stratification analysis, were used to estimate the adjusted odds ratio (AOR) with a 95% confidence interval (CI) for the health outcomes of telecommuters.

Results

Among the study population, 338 males and 318 females were reported to be telecommuters. The entirely adjusted regression model showed a positive association between telecommuting and anxiety (AOR = 2.82; 95% CI, 1.93–4.10), insomnia (AOR = 1.93; 95% CI, 1.27–2.92), fatigue (AOR = 1.76; 95% CI, 1.30–2.37), musculoskeletal pain (AOR = 1,76; 95% CI, 1.33–2.32), headache-eye strain (AOR = 1.94; 95% CI, 1.48–2.54), presenteeism (AOR = 1.66; 95% CI, 1.20–2.28) respectively. Gender difference was identified in that only female telecommuters had a higher risk of depression (AOR = 1.62; 95% CI, 1.04–2.53) and insomnia (AOR = 2.07; 95% CI, 1.26–3.41) than daily commuters in the adjusted model.

Conclusion

Telecommuting was significantly associated with an increased risk of various health problems among South Korean workers and females were identified as a more vulnerable group. Although further research is required to ascertain the causal relationship, public health intervention should be considered to prevent the negative effects of telecommuting.
Risk of ischemic and hemorrhagic stroke in relation to cold spells in four seasons
Authors: Vaičiulis Vidmantas , Jaakkola Jouni J. K. , Radišauskas Ričardas , Tamošiūnas Abdonas , Lukšienė Dalia , Ryti Niilo R. I.
Published: March 2023

Background

Cold winter weather increases the risk of stroke, but the evidence is scarce on whether the risk increases during season-specific cold weather in the other seasons. The objective of our study was to test the hypothesis of an association between personal cold spells and different types of stroke in the season-specific context, and to formally assess effect modification by age and sex.

Methods

We conducted a case-crossover study of all 5396 confirmed 25–64 years old cases with stroke in the city of Kaunas, Lithuania, 2000–2015. We assigned to each case a one-week hazard period and 15 reference periods of the same calendar days of other study years. A personal cold day was defined for each case with a mean temperature below the fifth percentile of the frequency distribution of daily mean temperatures of the hazard and reference periods. Conditional logistic regression was applied to estimate odds ratios (OR) and 95% confidence intervals (95% CI) representing associations between time- and place-specific cold weather and stroke.

Results

There were positive associations between cold weather and stroke in Kaunas, with each additional cold day during the week before the stroke increases the risk by 3% (OR 1.03; 95% CI 1.00–1.07). The association was present for ischemic stroke (OR 1.05; 95% CI 1.01–1.09) but not hemorrhagic stroke (OR 0.98; 95% CI 0.91–1.06). In the summer, the risk of stroke increased by 8% (OR 1.08; 95% CI 1.00–1.16) per each additional cold day during the hazard period. Age and sex did not modify the effect.

Conclusions

Our findings show that personal cold spells increase the risk of stroke, and this pertains to ischemic stroke specifically. Most importantly, cold weather in the summer season may be a previously unrecognized determinant of stroke.
Signet ring cell-like diffuse large B-cell lymphoma involving the breast: a case report
Authors: Zhang Lu , Min Qinqin , Bi Jiaxin , Yu Xuewen , Liang Yingying , Shao Mumin
Published: March 2023

Background

Diffuse large B-cell lymphoma (DLBCL) with signet ring cell components is extremely rare. Here, we present a case of DLBCL with signet ring cell components involving the breast, which can be easily confused with invasive lobular carcinoma of the breast or metastatic signet ring cell carcinoma of gastrointestinal origin.

Case presentation

A 66-year-old woman presented with a painless mass in her left breast. Enhanced magnetic resonance imaging (MRI) of the breast revealed a 42 × 29 × 28 mm mass in the left breast. Histological examination revealed a diffuse or scattered arrangement of round cells mixed with signet ring-like cells. Immunohistochemically, the neoplastic cells were positive for PAX-5, CD79a, CD20, Bcl-6, and MUM-1 but and negative for cytokeratin, ER, PR, E-cadherin, and P120. The Ki-67 proliferation index was approximately 70%. Fluorescence in situ hybridisation (FISH) demonstrated non-rearrangement of Bcl-2, Bcl-6, and c-MYC genes. Immunohistochemistry and FISH examination confirmed the diagnosis of DLBCL. Subsequently, immunofluorescence showed both IgM and IgG deposits in the signet ring-like lymphocytes. After confirming the diagnosis, the patient received four courses of CHOP (cyclophosphamide, doxorubicin, vincristine, and prednisolone) chemotherapy in a specialist hospital and achieved partial remission; however, she unfortunately died of secondary pneumocystis pneumonia infection 3 months later.

Conclusion

Malignant lymphoma with signet ring cell morphology is quite uncommon, and this variant can be a diagnostic pitfall. We emphasise that pathologists should consider lymphoma in the differential diagnosis of malignant breast tumours.
Association between malaria and household air pollution interventions in a predominantly rural area of Ghana
Authors: Asante Kwaku Poku , Wylie Blair J. , Oppong Felix B. , Quinn Ashlinn , Gyaase Stephaney , Lee Alison G. , Ae-Ngibise Kenneth Ayuurebobi , Burkart Katrin , Boamah-Kaali Ellen Abrafi , Kaali Seyram , Chillrud Steven , Kinney Patrick L. , Owusu-Agyei Seth , Jack Darby
Published: March 2023

Background

Though anecdotal evidence suggests that smoke from HAP has a repellent effect on mosquitoes, very little work has been done to assess the effect of biomass smoke on malaria infection. The study, therefore, sought to investigate the hypothesis that interventions to reduce household biomass smoke may have an unintended consequence of increasing placental malaria or increase malaria infection in the first year of life.

Methods

This provides evidence from a randomized controlled trial among 1414 maternal-infant pairs in the Kintampo North and Kintampo South administrative areas of Ghana. Logistic regression was used to assess the association between study intervention assignment (LPG, Biolite or control) and placental malaria. Finally, an extended Cox model was used to assess the association between study interventions and all episodes of malaria parasitaemia in the first year of infant’s life.

Results

The prevalence of placental malaria was 24.6%. Out of this, 20.8% were acute infections, 18.7% chronic infections and 60.5% past infections. The study found no statistical significant association between the study interventions and all types of placental malaria (OR = 0.88; 95% CI 0.59–1.30). Of the 1165 infants, 44.6% experienced at least one episode of malaria parasitaemia in the first year of life. The incidence of first and/or only episode of malaria parasitaemia was however found to be similar among the study arms.

Conclusion

The findings suggest that cookstove interventions for pregnant women and infants, when combined with additional malaria prevention strategies, do not lead to an increased risk of malaria among pregnant women and infants.
Single inferior parathyroid autotransplantation during total thyroidectomy with bilateral central lymph node dissection for papillary thyroid carcinoma: a retrospective cohort study
Authors: Dong Zhizhong , Liu Wen , Peng Ying , Zhan Xiangxiang , Su Yanjun , Diao Chang , Cheng Ruochuan
Published: March 2023

Background

Management of the inferior parathyroid gland using total thyroidectomy (TT) with central lymph node dissection (CLND) is still controversial. Therefore, we evaluated the safety and effectiveness of single inferior parathyroid autotransplantation.

Methods

The clinical data of patients with papillary thyroid carcinoma (PTC) who underwent TT with bilateral CLND from January 2018 to December 2020 were collected. Quality of life (QoL) was assessed using the Chinese version of the EORTC QLQ-C30 and THYCA-QOL. The patients were divided into an autotransplantation group and a preservation group according to whether a single inferior parathyroid gland was transplanted. The incidence of permanent hypoparathyroidism, the number of resected central lymph nodes (CLNs), the rate of recurrence reoperation, the rate of radioactive iodine (RAI) treatment, and the QoL score were compared between the two groups.

Results

A total of 296 patients were included in the study; there were 99 patients in the autotransplantation group and 197 in the preservation group. The incidence of permanent hypoparathyroidism was 3.0% (3/99) and 4.6% (9/197) in the autotransplantation and preservation groups, respectively (P = 0.532). The median number of resected CLNs was 12 (8–17) and 10 (6–14) in the autotransplantation and preservation groups, respectively (P = 0.015). No reoperations were performed for patients with CLN recurrence, and the rates of lateral lymph node (LLN) recurrence reoperation were 2.0% (2/99) and 3.6% (7/197) in the autotransplantation and preservation groups, respectively (P = 0.473). The RAI treatment rates were 12.1% (12/99) and 22.3% (44/197) in the autotransplantation and preservation groups, respectively (P = 0.034). A total of 276 questionnaires were recovered, including 84 in the autotransplantation group and 192 in the preservation group. The QoL of the two groups of patients is similar (P > 0.05).

Conclusion

Single inferior parathyroid autotransplantation during thyroidectomy can be used to prevent permanent hypoparathyroidism and can enable more extensive CLND.
A retrospective study of adjuvant proton radiotherapy for breast cancer after lumpectomy: a comparison of conventional-dose and hypofractionated dose
Authors: Hong ZhengShan , Yang ZhaoZhi , Mei Xin , Li Ping , Bao Cihang , Wang Zheng , Cai Xin , Ming Xue , Wang WeiWei , Guo XiaoMao , Yu XiaoLi , Zhang Qing
Published: March 2023

Purpose

This study aimed to compare the adverse reactions of conventional-dose and hypofractionated dose of proton therapy for breast cancer.

Materials and methods

Breast cancer patients treated with proton radiotherapy in conventional-dose or hypofractionated dose were studied retrospectively.

Result

From January 2017 to December 2019, our center treated 50 patients following lumpectomy with proton radiotherapy. According to the AJCC 8th Edition standard, there were stage I in 26 patients, stage II in 22 patients, and stage III in 2 patients. A total of 14 patients received intensity-modulated proton therapy at a dose of 50 Gy in 25 fractions, followed by a 10 Gy 4 fractionated boost to the lumpectomy cavity, while 36 received 40.05 Gy in 15 fractions, simultaneous integrated boost (SIB) 48 Gy to the lumpectomy cavity. Median follow-up time for 40.05 Gy group was 35.6 months (15–43 months). Median follow-up time for 50 Gy group was 46.8 months (36–68 months). For acute toxicity, the grade 1 and 2 radiodermatitis in conventional-dose group were 35.7% and 57.1%, respectively. In hypofractionated dose group, the grade 1 and 2 radiodermatitis were 91.7% and 8.3%, respectively. The radiodermatitis is hypofractionneted dose better than conventional-dose significantly. Grade 1 radiation-induced esophagitis in conventional-dose group and hypofractionated dose group were 85.71% and 60%, respectively. For late toxicity, no patients developed radiation-induced pneumonitis and rib fracture in conventional-dose group. Three patients presented grade 1 pneumonitis; one patient presented graded 2 pneumonitides and two patients presented rib fracture in hypofractionated dose group. One presented hypothyroidism in hypofractionated dose group. All patients were satisfied with breast shape. The one- and two-year OS and DFS for conventional-dose group were 100 and 100; 100 and 92.9%, respectively. The one- and two-year OS and DFS for hypofractionated dose group were 100 and 100; 100 and 100%, respectively.

Conclusion

Proton radiation therapy can significantly reduce the normal tissue dose in breast cancer patients' hearts, lungs, and other organs. Hypofractionated proton therapy shortens the treatment course with mild radiation-related adverse effects, and has a better effect on addressing the acute adverse reactions than conventional proton radiotherapy.
The predictors of food security and dietary diversity among internally displaced persons’ children (6–59 months) in Bamenda health district, Cameroon
Authors: Tambe Ayuk Betrand , Akeh Mbah Larissa , Tendongfor Nicholas , Dhlamini Thembekile , Chipili Given , Mbhenyane Xikombiso
Published: March 2023

Background

Malnutrition remains a major cause of morbidity and mortality amongst children in displaced settings. Nutrition at this stage is crucial for the growth and development of the child. It is estimated that 41 million children under 5 years are obese/overweight, 159 million are stunted and 50 million are wasted worldwide. This study aimed to determine the prevalence and predictors of food insecurity and dietary diversity among internally displaced persons’ children from 6 to 59 months.

Methodology

A cross sectional community-based study was conducted on 395 children aged 6–59 months from May 2021-June 2021. A multistage cluster sampling method was used to select the study participants. A validated structured questionnaire was used to collect data on sociodemographic characteristics, food security and dietary diversity. Predictors of food insecurity and dietary diversity were identified using logistic regression. The outputs were presented using adjusted odd ratio (AOR) with 95% confidence interval (CI).

Results

The study results revealed that the level of household food insecurity was 91.6%, at risk of experiencing hunger (3.3%) and food secure (5.5%). Participants had mean dietary diversity score of 3.6 food groups, 51.6% had a low or inadequate dietary diversity and 48.4% had adequate dietary diversity. Children who were from households with monthly income of $101- $200 US dollars and had been displaced just once were 79% (AOR: 0.21, 95%CI: 0.07–0.60) and 84% (AOR: 0.16, 95%CI: 0.05–0.50) less likely to be food insecure compared to their counterparts respectively. While households with participants who Walked ≥ 10 min to fetch drinking water (AOR: 11.61 95%CI: 2.39–52.08) were more likely food insecure. In addition, household monthly income of ≥ $100 US dollars (AOR: 0.20, 95%CI: 0.07–0.56) had a reduced chance of providing low diversified food. Households that had received social grants (AOR: 2.15, 95%CI: 1.38–3.49) and walked ≥ 10 min to fetch drinking water (AOR: 2.43, 95%CI: 1.48–3.98) had a higher chance of providing low diversified food.

Conclusion

Dietary diversity and household food insecurity was unacceptably low and high respectively among internally displaced children. Policymakers should prioritize strengthening both nutrition sensitive and specific activities that contribute to reduction of food insecurity and consumption of unbalance diet.
Ten-month follow-up of patients with covid-19 temporally related multi-system inflammatory syndrome in children: the experience of the children hospital of Palermo
Authors: Maggio Maria Cristina , Giordano Salvatore , Failla Maria Concetta , Campione Martina Gioacchina , Alaimo Annalisa , Corsello Giovanni
Published: March 2023

Background

In Sicily, the first wave of COVID-19 showed a low epidemic impact in paediatric population, while the second and the third waves had a higher impact on clinical presentation of COVID-19 in children and a significantly higher severe outcome in patients with multisystem inflammatory syndrome in children (MIS-C), with a frequent life-threatening progression.

Methods

We describe a cohort of 22 Sicilian children (11 M; 11 F; age: 1.4–14 years), presenting with clinical features compatible with MIS-C. Patients with negative swab had a history of recent personal or parental infection.

Results

The following diagnostic criteria were detected: fever (100%); cheilitis and/or pharyngeal hyperaemia (86%); latero-cervical lymphadenitis (82%); rash (73%); abdominal pain and/or vomiting and/or diarrhoea (64%); conjunctivitis (64%); hands and feet oedema (18%). 59% showed cardiac involvement (6 pericardial effusion; 8 mitral valve insufficiency; 4 insufficiency of two valves; 3 coronary artery lesions (CAL)). In all the patients, treatment was started within 72 h after the admission, with intravenous immunoglobulins (IVIG) (2 g/Kg/dose), methylprednisolone (2 mg/Kg/day in 73% of patients; 30 mg/Kg/day for 3 days, followed by 2 mg/Kg/day in 27% of patients). Two patients were treated with enoxaparin. Two patients with shock, were additionally treated with vasoactive drugs, albumin, diuretics. Cardiac involvement evolved into the complete resolution of lesions in most of the patients. All the patients were included in a follow-up, to investigate on clinical outcome and resolution of organ involvement. Cardiac valve insufficiency persisted only in 18% of children, CAL persisted only in 33% of children with coronary involvement, however without the evolution into aneurisms.

Conclusions

The preferred treatment strategy was more aggressive at the diagnosis of MIS-C, to block the cytokine cascade. Most of our patients, in fact, received a first-line treatment with IVIG and steroids. This approach could explain the favourable prognosis, the rapid restoring of cardiac function also in patients with MAS or shock, and the good outcome during the 10 months follow-up in all the patients.
Human cortical spheroids with a high diversity of innately developing brain cell types
Authors: De Kleijn Kim M. A. , Zuure Wieteke A. , Straasheijm Kirsten R. , Martens Marijn B. , Avramut M. Cristina , Koning Roman I. , Martens Gerard J. M.
Published: March 2023

Background

Three-dimensional (3D) human brain spheroids are instrumental to study central nervous system (CNS) development and (dys)function. Yet, in current brain spheroid models the limited variety of cell types hampers an integrated exploration of CNS (disease) mechanisms.

Methods

Here we report a 5-month culture protocol that reproducibly generates H9 embryonic stem cell-derived human cortical spheroids (hCSs) with a large cell-type variety.

Results

We established the presence of not only neuroectoderm-derived neural progenitor populations, mature excitatory and inhibitory neurons, astrocytes and oligodendrocyte (precursor) cells, but also mesoderm-derived microglia and endothelial cell populations in the hCSs via RNA-sequencing, qPCR, immunocytochemistry and transmission electron microscopy. Transcriptomic analysis revealed resemblance between the 5-months-old hCSs and dorsal frontal rather than inferior regions of human fetal brains of 19–26 weeks of gestational age. Pro-inflammatory stimulation of the generated hCSs induced a neuroinflammatory response, offering a proof-of-principle of the applicability of the spheroids.

Conclusions

Our protocol provides a 3D human brain cell model containing a wide variety of innately developing neuroectoderm- as well as mesoderm-derived cell types, furnishing a versatile platform for comprehensive examination of intercellular CNS communication and neurological disease mechanisms.
Inflammatory stimulation of astrocytes affects the expression of miRNA-22-3p within NSCs-EVs regulating remyelination by targeting KDM3A
Authors: Han Tianyu , Song Peiwen , Wu Zuomeng , Wang Cancan , Liu Yunlei , Ying Wang , Li Kaixuan , Shen Cailiang
Published: March 2023

Background

Endogenous neural stem cells (NSCs) are critical for the remyelination of axons following spinal cord injury (SCI). Cell–cell communication plays a key role in the regulation of the differentiation of NSCs. Astrocytes act as immune cells that encounter early inflammation, forming a glial barrier to prevent the spread of destructive inflammation following SCI. In addition, the cytokines released from astrocytes participate in the regulation of the differentiation of NSCs. The aim of this study was to investigate the effects of cytokines released from inflammation-stimulated astrocytes on the differentiation of NSCs following SCI and to explore the influence of these cytokines on NSC–NSC communication.

Results

Lipopolysaccharide stimulation of astrocytes increased bone morphogenetic protein 2 (BMP2) release, which not only promoted the differentiation of NSCs into astrocytes and inhibited axon remyelination in SCI lesions but also enriched miRNA-22-3p within extracellular vesicles derived from NSCs. These miRNA-22 molecules function as a feedback loop to promote NSC differentiation into oligodendrocytes and the remyelination of axons following SCI by targeting KDM3A.

Conclusions

This study revealed that by releasing BMP2, astrocytes were able to regulate the differentiation of NSCs and NSC–NSC communication by enriching miRNA-22 within NSC-EVs, which in turn promoted the regeneration and remyelination of axons by targeting the KDM3A/TGF-beta axis and the recovery of neurological outcomes following SCI.
Generalization of the bisection method and its applications in nonlinear equations
Authors: Gulshan Ghazala , Budak Hüseyin , Hussain Rashida , Sadiq Asad
Published: March 2023

The aim of the current work is to generalize the well-known bisection method using quantum calculus approach. The results for different values of quantum parameterqare analyzed, and the rate of convergence for each$q\in (0,1)$q∈(0,1)is also determined. Some physical problems in engineering are resolved using the QBM technique for various values of the quantum parameterqup to three iterations to examine the validity of the method. Furthermore, it is proven that QBM is always convergent and that for each interval there exists$q\in (0,1)$q∈(0,1)for which the first approximation of root coincides with the precise solution of the problem.
A better use of fertilizers is needed for global food security and environmental sustainability
Authors: Penuelas Josep , Coello Fernando , Sardans Jordi
Published: March 2023

The massive use of fertilizers during the last decades allowed a great increase in the global capacity of food production. However, in the last years, several studies highlight the inefficiency and country asymmetries in the use of these fertilizers that generated environmental problems, soil nutritional imbalances and not optimal food production. We have aimed to summarize this information and identify and disentangle the key caveats that should be solved. Inadequate global management of fertilization produces areas with serious nutrient deficits in croplands linked with insufficient access to fertilizers that clearly limit food production, and areas that are overfertilized with the consequent problems of environmental pollution affecting human health. A more efficient use of nitrogen (N), phosphorus (P) and potassium (K) fertilizers for food security while preserving the environment is thus needed. Nutrient imbalances, particularly the disequilibrium of the N:P ratio due to the unbalanced release of N and P from anthropogenic activities, mainly by crop fertilization and expanding N-fixing crops that have continuously increased the soil N:P ratio, is another issue to resolve. This imbalance has already affected several terrestrial and aquatic ecosystems, altering their species composition and functionality and threatening global biodiversity. The different economic and geopolitical traits of these three main macronutrient fertilizers must be considered. P has the fewest reserves, depending mostly on mineable efforts, with most of the reserves concentrated in very few countries (85% in Morocco). This problem is a great concern for the current and near-future access to P for low-income countries. N is instead readily available due to the well-established and relatively low-cost Haber–Bosch synthesis of ammonium from atmospheric N2, which is increasingly used, even in some low-income countries producing an increasing imbalance in nutrient ratios with the application of P and K fertilizers. The anthropogenic inputs of these three macronutrients to the environment have reached the levels of the natural fluxes, thereby substantially altering their global cycles. The case of the excess of N fertilization is especially paradigmatic in several areas of the world, where continental water sources have become useless due to the higher nitrate concentrations. The management of N, P and K fertilizers is thus in the center of the main dichotomy between food security and environmentally driven problems, such as climate change or eutrophication/pollution. Such a key role demands new legislation for adopting the well-known and common-sense 4R principle (right nutrient source at the right rate, right time and right place) that would help to ensure the appropriate use of nutrient resources and the optimization of productivity.
New Anchorage Technique for GFRP Flexural Strengthening of Concrete Beams Using Bolts-End Anchoring System
Authors: Yahiaoui Djarir , Boutrid Abdelaziz , Saadi Mohamed , Mamen Belgacem , Bouzid Tayeb
Published: March 2023

The concept of external glass FRP composite confinement is a current process for strengthening concrete beams subjected to static loads. End anchorage glass FRP composites of 80 mm width and 90–130 mm length with different thicknesses (2.4 and 4.8 mm) have been fixed at the bottom of beams with bolts of various diameters (6 and 10 mm). For this purpose, the behavior of beams strengthened with bolt-end anchoring glass fiber polymer composites (BEGFPC) has been analyzed. It is concluded that the load capacity of the BEGFPC beams is improved by increasing the end-anchorage glass FRP composite thickness (about 98–188%). In addition, the BEGFPC system with bolts of 6 mm diameter has significantly improved the flexibility of beams. In contrast, the 10 mm bolts in diameter give a high ultimate load, whatever their quantity. Therefore, combining bolts with diameters of 6 and 10 mm would be the best solution for increasing the ultimate load and ductility of the retrofitted beams. Depending on the number and bolts' arrangement, there is also an enhancement in the crack patterns by changing from intermediate flexural failure to shear failure in beams.
Linking movement and dive data to prey distribution models: new insights in foraging behaviour and potential pitfalls of movement analyses
Authors: Florko Katie R. N. , Shuert Courtney R. , Cheung William W. L. , Ferguson Steven H. , Jonsen Ian D. , Rosen David A. S. , Sumaila U. Rashid , Tai Travis C. , Yurkowski David J. , Auger-Méthé Marie
Published: March 2023

Background

Animal movement data are regularly used to infer foraging behaviour and relationships to environmental characteristics, often to help identify critical habitat. To characterize foraging, movement models make a set of assumptions rooted in theory, for example, time spent foraging in an area increases with higher prey density.

Methods

We assessed the validity of these assumptions by associating horizontal movement and diving of satellite-telemetered ringed seals (Pusa hispida)—an opportunistic predator—in Hudson Bay, Canada, to modelled prey data and environmental proxies.

Results

Modelled prey biomass data performed better than their environmental proxies (e.g., sea surface temperature) for explaining seal movement; however movement was not related to foraging effort. Counter to theory, seals appeared to forage more in areas with relatively lower prey diversity and biomass, potentially due to reduced foraging efficiency in those areas.

Conclusions

Our study highlights the need to validate movement analyses with prey data to effectively estimate the relationship between prey availability and foraging behaviour.
Onset timing and duration of augmented renal clearance in a mixed intensive care unit
Authors: Mikami Ryusei , Hayakawa Mineji , Imai Shungo , Sugawara Mitsuru , Takekuma Yoh
Published: March 2023

Background

Augmented renal clearance (ARC) is associated with lower blood plasma concentrations of renally excreted drugs; however, its time course is unknown. The current study aimed to determine the onset timing/duration of ARC, its risk factors, and its association with clinical outcomes by continuous monitoring of urinary creatinine clearance (CrCl) in critically ill patients.

Methods

Data were retrospectively obtained from the medical records of 2592 critically ill patients admitted to the intensive care unit (ICU) from January 2019 to June 2022 at a tertiary emergency hospital. Among these, patients with continuously measured urinary CrCl were selected and observed over time. We evaluated the onset timing and duration of ARC by plotting Kaplan–Meier curves. Furthermore, by multivariate analyses, factors associated with the onset and persistence of ARC were analyzed, and the association between the ARC time course and clinical outcomes was evaluated.

Results

The prevalence of ARC was 33.4% (245/734). ARC onset was within 3 days of admission in approximately half of the cases, and within 1 week in most of the other cases. In contrast, the persistence duration of ARC varied widely (median, 5 days), and lasted for more than a month in some cases. Multivariate analysis identified younger age, male sex, lower serum creatinine at admission, admission with central nervous system disease, no medical history, use of mechanically assisted ventilation, and vasopressor use as onset factors for ARC. Furthermore, factors associated with ARC persistence such as younger age and higher urinary CrCl on ARC day 1 were detected. The onset of ARC was significantly associated with reduced mortality, but persistent of ARC was significantly associated with fewer ICU-free days.

Conclusions

Despite the early onset of ARC, its duration varied widely and ARC persisted longer in younger patients with higher urinary CrCl. Since the duration of ARC was associated with fewer ICU-free days, it may be necessary to consider a long-term increased-dose regimen of renally excreted drugs beginning early in patients who are predicted to have a persistent ARC.
Shallow resistivity structure around the 2018 craters of Mt. Motoshirane of Kusatsu-Shirane Volcano, Japan, revealed by audio-frequency magnetotellurics
Authors: Honda Asami , Kanda Wataru , Koyama Takao , Takakura Shinichi , Matsunaga Yasuo , Nishizawa Tatsuji , Ikezawa Satoshi
Published: March 2023

In 2018, a phreatic eruption occurred at Mt. Motoshirane, a pyroclastic cone group of the Kusatsu-Shirane Volcano in central Japan. The eruption was abrupt, and no signs of volcanic activity have been observed in recent years, in contrast to the other major pyroclastic cone group, Mt. Shirane, which hosts the active crater lake and has endured repeated phreatic eruptions. To understand the mechanism of the eruption at Mt. Motoshirane, information on the shallow hydrothermal system, which is thought to be the source region of phreatic eruptions, is required; however, few studies have been conducted on this particular cone group. In this study, we conducted an audio-frequency magnetotelluric survey in 2020 to reveal the shallow resistivity structure around the 2018 craters. A three-dimensional resistivity structure model showed generally two layers, with high resistivities at shallow depths overlain by low resistivities underneath. The boundary between the layers corresponded to the top boundary of the Neogene basement rocks. These low resistivities were not found beneath the 2018 craters; therefore, part of the Neogene basement rocks could have been lost by the eruption beneath the 2018 craters. This is consistent with the geochemical study on the mineral assemblage of the erupted deposits, which suggested that the explosions reached the depth of the basement.

Graphical Abstract