bluuebunny/crossref_metadata_2025 huggingface dataset:
Late-Autumn Ramet Sprouting of Three Arable Creeping Perennial Weed Species
Authors: Tørresen Kirsten S. , Gerowitt Bärbel
Published: September 2022

Elymus repens (L.) Gould), Cirsium arvense (L.) Scop. and Sonchus arvensis L. are important arable creeping perennial weeds in Europe. These are clonal plants with subterranean reproductive organs (E. repens, rhizomes, the two dicots, horizontal creeping roots) sprouting from ramets. We tested the sprouting ability and early growth of ramet sprouts at temperatures typical for Nordic autumn climate and with different preconditions of the mother plant (time in autumn, mother plant age, climate change experiences of the mother plants (two experiments)). The species reacted differently, with S. arvensis not sprouting at all, and C. arvense ramets sprouting at higher temperatures than those of E. repens, which sprouted at all tested temperatures. Plant age affected only the ramet sprout biomass of E. repens. Climate change during mother plant growth only affected C. arvense, with the highest above-ground biomass of the sprouted ramets at an elevated temperature and ambient CO2. Testing earlier in autumn showed more sprouting and biomass for C. arvense and E. repens than testing later in the season. The observed temperature responses confirmed more and bigger sprouts with higher autumn temperatures. Controlling the sprouted ramets in autumn is easier for E. repens than for C. arvensis. Due to their low/no sprouting ability in autumn, the ramets of S. arvensis cannot be controlled in autumn.
The Welfare of Traveller and Gypsy Owned Horses in the UK and Ireland
Authors: Rowland Marie , Hudson Neil , Connor Melanie , Dwyer Cathy , Coombs Tamsin
Published: September 2022

Travellers and Gypsies are recognised ethnic groups in the UK and Ireland. Horse ownership is an important cultural tradition, however, practices associated with poor welfare are often perceived to be linked to these horse owning communities. Despite this, empirical studies on the welfare status of Traveller and Gypsy owned horses are lacking. To determine the welfare status of Traveller and Gypsy owned horses, 104 horses were assessed using a bespoke horse welfare protocol. This protocol assessed animal, resource and management-based measures. In addition, Qualitative Behaviour Assessment (QBA) identified horses’ emotional state. Results indicated that 81% of horses had an optimal body condition score, with no horse recorded as very thin/fat. The absence of limb conditions (95%), ocular (98%) and nasal (93%) discharges were evident in most horses, and 81% of horses responded positively to the voluntary animal approach test. The most commonly observed welfare issues were hoof neglect (27%), with hoof cracks/breakages (19%) being the most prevalent. QBA indicated that positive emotional states were more commonplace than negative. A relationship between QBA and other horse welfare measures was observed, e.g., improved mood was associated with better water availability. This research provides novel data in the under-researched area of the welfare of Traveller and Gypsy owned horses and counters perceptions of a poor welfare state in this group of horses.
Dual Roles of Extracellular Histone H3 in Host Defense: Its Differential Regions Responsible for Antimicrobial and Cytotoxic Properties and Their Modes of Action
Authors: Tanaka Yuri , Yamanaka Nanako , Koyano Izumi , Hasunuma Itaru , Kobayashi Tetsuya , Kikuyama Sakae , Iwamuro Shawichi
Published: September 2022

Extracellular histones play a dual role—antimicrobial and cytotoxic—in host defense. In this study, we evaluated the antimicrobial and cytotoxic activities of histone H3 and identified the responsible molecular regions for these properties. Broth microdilution assays indicated that histone H3 exhibits growth inhibitory activity against not only Gram-negative and -positive bacteria but also fungi. Observations under scanning electron microscopy (SEM) revealed that histone H3 induced morphological abnormalities on the cell surface of a wide range of reference pathogens. MTT assays and SEM observations indicated that histone H3 has strong cytotoxic and cell lytic effects on mammalian normal, immortal, and tumor cell lines. Assays using synthetic peptides corresponding to fragments 1–34 (H3DP1), 35–68 (H3DP2), 69–102 (H3DP3), and 103–135 (H3DP4) of histone H3 molecule demonstrated that its antimicrobial activity and cytotoxicity are elicited by the H3DP2 and H3DP3 protein regions, respectively. Enzyme-linked endotoxin binding assays indicated that histones H3 and H3DP1, H3DP2, and H3DP4, but not H3DP3, exhibited high affinities toward lipopolysaccharide and lipoteichoic acid. Our findings are expected to contribute to the development of new histone H3-based peptide antibiotics that are not cytotoxic.
Clonal Dissemination of Extended-Spectrum Cephalosporin-Resistant Enterobacterales between Dogs and Humans in Households and Animal Shelters of Romania
Authors: Cozma Andreea Paula , Rimbu Cristina Mihaela , Zendri Flavia , Maciuca Iuliana Elena , Timofte Dorina
Published: September 2022

Faecal carriage of extended-spectrum cephalosporin-resistant (ESC-R) Enterobacterales in healthy pets is a concerning issue. This study aimed to determine the prevalence, genetic background, and potential for interspecies transmission of these bacteria between dogs and humans within the same household (HH) or shelter environment in Romania. Faecal samples (n = 263) collected from healthy dogs (n = 102), their owners (n = 32), as well as dogs (n = 110) and staff (n = 19) from dog shelters, were screened for ESC-R carriage. Clonal relatedness of canine and human Escherichia coli isolates was established using Fourier Transform Infrared Spectroscopy (FTIR), followed by Illumina WGS of selected isolates. The highest prevalence of ESC-R Enterobacterales faecal carriage was identified in staff working at dog shelters (78.9%), followed by dogs from households (44.11%), dog owners (43.7%), and dogs from shelters (27%). FTIR identified 15 clusters of closely related E. coli isolates, including dog and human isolates from the same environment. Co-carriage of ESC-R isolates in both the dog and owner was identified in 12 HHs (37.5%), with two HHs (6%) having both the owner and dog carrying isolates with identical FTIR spectra, phylogroup, resistance genes, and Inc plasmids. Major ExPEC lineages such as ST127, ST10, ST155, and ST88 were detected in human and dog isolates. Our study revealed a high prevalence of faecal ESC-R E. coli carriage in both dogs and humans from Romanian households and shelters, where bidirectional clonal transmission between humans and dogs is likely. Furthermore, we identified ESC-R Enterobacterales co-carriage in people and dogs sharing the same environment using FTIR, demonstrating its value in AMR surveillance for humans and animals.
Bacillus amyloliquefaciens Lysate Ameliorates Photoaging of Human Skin Fibroblasts through NRF2/KEAP1 and TGF-β/SMAD Signaling Pathways
Authors: Zhang Yongtao , Zhao Jingsha , Jiang Yanbing , Wang Dongdong , Zhao Dan , Wang Changtao , Li Meng
Published: September 2022

More and more research in dermatology and cosmetic science is devoted to the development and application of postbiotic raw materials. In order to explore the anti-photoaging efficacy and application prospect of Bacillus amyloliquefaciens lysate (BAL1) on the skin, we used 16 J/cm2 UVA stimulation of human embryonic fibroblasts (CCC-ESF-1) to establish a UVA photodamage model to investigate the anti-photoaging efficacy of BAL1 and its mechanism of action. In this study, we found that BAL1 activated the transcription of downstream antioxidant enzyme genes mainly by promoting the nuclear displacement of NF-E2-related factor 2 (Nrf2) within CCC-ESF-1, thus increasing the antioxidant capacity of antioxidant enzymes to scavenge excessive reactive oxygen species in cells. Meanwhile, BAL1 promoted intracellular TGF-β/Smad signaling pathway and reduced matrix metalloproteinase expression to alleviate the degradation of extracellular matrix. In conclusion, the results of this study demonstrate the potential benefit of BAL1 in protecting the skin against UVA-induced photoaging and highlight the potential of BAL1 in skin photoprotection.
Experimental Dynamic Response of a Multi-Story Frame Structure Equipped with Non-Conventional TMD Implemented via Inter-Story Isolation
Authors: Basili Michela , Angelis Maurizio De
Published: September 2022

A shaking table experiment conducted on a multi-degrees-of-freedom frame structure equipped with a non-conventional tuned mass damper (TMD) is presented. The non-conventional TMD is characterized by a high mass ratio, without adding further structural masses, and is realized via inter-story isolation. The structure top story mass of a four-story steel frame structure is isolated and converted into tuned mass, connecting to the substructure with two high damping rubber bearings placed in series. Aspects related to the dynamic structural response as well as the seismic effectiveness assessment of a non-conventional TMD are addressed. Three structural configurations are tested: the reference four-story structure, the three-story intermediate structure, and the three-story structure equipped with a non-conventional TMD. The input motion conditions considered are: white noise, sine sweep, and natural earthquakes. Through experiments, structural identification is carried out and different dynamic behaviors emerge for the configurations tested. The nonlinear effects provoked on the structure by the adopted isolators are investigated, showing high dissipative capabilities in a wide range of amplitudes of the excitation. It is demonstrated that a non-conventional TMD is a smart control strategy useful for enhancing structural vibration mitigation.
Automated Hybrid Model for Detecting Perineural Invasion in the Histology of Colorectal Cancer
Authors: Jung Jiyoon , Kim Eunsu , Lee Hyeseong , Lee Sung Hak , Ahn Sangjeong
Published: September 2022

Perineural invasion (PNI) is a well-established independent prognostic factor for poor outcomes in colorectal cancer (CRC). However, PNI detection in CRC is a cumbersome and time-consuming process, with low inter-and intra-rater agreement. In this study, a deep-learning-based approach was proposed for detecting PNI using histopathological images. We collected 530 regions of histology from 77 whole-slide images (PNI, 100 regions; non-PNI, 430 regions) for training. The proposed hybrid model consists of two components: a segmentation network for tumor and nerve tissues, and a PNI classifier. Unlike a “black-box” model that is unable to account for errors, the proposed approach enables false predictions to be explained and addressed. We presented a high performance, automated PNI detector, with the area under the curve (AUC) for the receiver operating characteristic (ROC) curve of 0.92. Thus, the potential for the use of deep neural networks in PNI screening was proved, and a possible alternative to conventional methods for the pathologic diagnosis of CRC was provided.
Hair Growth Booster Effects of Micro-Needling with Low-Level Led Therapy and Growth Factors on Subjects Treated with Finasteride®
Authors: Gentile Pietro , Ki Mun-Sang
Published: September 2022

A procedure based on Microneedling (MND) with Low-Level Led Therapy (LLLT) and Growth Factors (GFs) could be a booster for hair re-growth (HRG) in patients assuming Finasteride®. The study examined the clinical outcomes of a multicentric, observational, retrospective, case-series investigation in which MND with LLLT and GFs was applied to patients suffering from androgenic alopecia (AGA) who were prescribed Finasteride®. Twenty-one patients were initially enrolled, of which seventeen males were classified in stage II–VI by the Norwood–Hamilton scale, and four females were classified in stage II–III by the Ludwig scale. One male patient was excluded after screening (exclusion and inclusion criteria evaluation). Twenty patients were analyzed, of which ten patients’ hair growth has stalled after taking Finasteride®, and ten patients did not achieve good results from Finasteride®. HRG assessment was evaluated with photography, physician’s, and patient’s global assessment scale, in addition to standardized phototrichograms during a short follow-up: T0—baseline, T1—20 weeks (wks). A statistically significant improvement in HRG (p = 0.0822) and an increase in hair density of 19 ± 2 hairs/cm2 at T1 after 20 wks (20 wks vs. 0 wks) in the targeted area over baseline (74 ± 2 hairs/cm2 at T1 versus 55 ± 2 hairs/cm2 at baseline) were reported and described as encouraging results. The effectiveness of MND with LLLT and GFs use was demonstrated in patients whose hair growth stalled after taking Finasteride® and in patients who did not achieve good results from Finasteride®.
The Use of Ultrasound in Shaping the Properties of Ice Cream with Oleogel Based on Oil Extracted from Tomato Seeds
Authors: Nazarewicz Sybilla , Kozłowicz Katarzyna , Kobus Zbigniew , Gładyszewska Bożena , Matwijczuk Arkadiusz , Ślusarczyk Lidia , Skrzypek Tomasz , Sujka Monika , Kozłowicz Natalia
Published: September 2022

In this study, the possibility of using ultrasound technology as an alternative to traditional pasteurization and homogenization in ice cream production was presented. Three types of ice cream with different proportions of oleogel (5, 6, and 7%) prepared using tomato seed oil were studied. The fatty acid contents of the oil were analyzed. Using chemical analysis, dry matter, fat, protein, dietary fiber, ash, and pH of the ice cream samples were determined. The physical analysis included analysis of the ice cream samples using a differential scanning calorimeter (DSC) and determination of their first drop time, complete melting time, overrun, viscosity, hardness, and adhesiveness. The structure of the samples was evaluated using scanning electron microscopy. Fourier transform infrared spectroscopy spectra were measured using a dedicated QATR-S Single-Reflection ATR ACCESSORY with a diamond prism. With the increase in the proportion of oleogels, the fat and carbohydrate contents, the amount of freezable water, and the overrun of the samples were increased, whereas their viscosity and hardness were decreased. Oleogels were found to be a promising alternative to fat in ice cream rich in unsaturated fatty acids, and the ice cream samples prepared using ultrasound pasteurization showed lower overrun and viscosity and higher hardness.
A Fusion Multi-Strategy Marine Predator Algorithm for Mobile Robot Path Planning
Authors: Yang Luxian , He Qing , Yang Liu , Luo Shihang
Published: September 2022

Path planning is a key technology currently being researched in the field of mobile robotics, but traditional path planning algorithms have complex search spaces and are easily trapped in local minima. To solve the above problems and obtain the global optimal path of the mobile robot, a fusion multi-strategy marine predator algorithm (FMMPA) is proposed in this paper. The algorithm uses a spiral complex path search strategy based on Archimedes’ spiral curve for perturbation to expand the global exploration range, enhance the global search ability of the population and strengthen the steadiness of the algorithm. In addition, nonlinear convex decreasing weights are introduced to balance the ability of the algorithm for global exploration and local exploitation to achieve dynamic updating of the predator and prey population positions. At the same time, the golden sine algorithm idea is combined to update the prey position, narrow the search range of the predator population, and improve the convergence accuracy and speed. Furthermore, the superiority of the proposed FMMPA is verified by comparison with the original MPA and several well-known intelligent algorithms on 16 classical benchmark functions, the Wilcoxon rank sum test and part of the CEC2014 complex test functions. Finally, the feasibility of FMMPA in practical application optimization problems is verified by testing and analyzing the mobile robot path planning application design experiments.
Machine Learning Techniques and Systems for Mask-Face Detection—Survey and a New OOD-Mask Approach
Authors: Hu Youwen , Xu Yicheng , Zhuang Huiping , Weng Zhenyu , Lin Zhiping
Published: September 2022

Mask-face detection has been a significant task since the outbreak of the COVID-19 pandemic in early 2020. While various reviews on mask-face detection techniques up to 2021 are available, little has been reviewed on the distinction between two-class (i.e., wearing mask and without mask) and three-class masking, which includes an additional incorrect-mask-wearing class. Moreover, no formal review has been conducted on the techniques of implementing mask detection models in hardware systems or mobile devices. The objectives of this paper are three-fold. First, we aimed to provide an up-to-date review of recent mask-face detection research in both two-class cases and three-class cases, next, to fill the gap left by existing reviews by providing a formal review of mask-face detection hardware systems; and to propose a new framework named Out-of-distribution Mask (OOD-Mask) to perform the three-class detection task using only two-class training data. This was achieved by treating the incorrect-mask-wearing scenario as an anomaly, leading to reasonable performance in the absence of training data of the third class.
Rebar Fabrication Plan to Enhance Production Efficiency for Simultaneous Multiple Projects
Authors: Hong Eunbin , Yi June-Seong , Son JeongWook , Hong MinYoung , Jang YeEun
Published: September 2022

A discrete-event simulation (DES) model was developed to enhance the reinforcing bar (rebar) fabrication efficiency for multiple simultaneous projects at different sites. The production volume and procedure of the actual rebar fabrication plant were compared to the simulation model to ensure its accuracy. By determining the loss rate and necessary processing time, the fabrication plan was then optimized. The rebar type and machine features, which influence the loss rate and time required for rebar fabrication, were configured as the parameters in a discrete-event simulation model. The model considers a situation in which a rebar fabrication plant simultaneously delivers rebars to multiple sites. In this manner, the model can quantify the loss rate and time required in the fabrication process. The determination of the loss rate according to the import ratio of raw steel, site combination, and length can help optimize the rebar fabrication plan and increase work efficiency. In the considered scenario, a two-site combination and import ratio of raw steel of 2:1 (8 m:10 m) was noted to corresponded to the maximum decrease in the loss rate and required time. By extending the proposed approach to the complete rebar process (processing–transportation–construction), the plant member production process can be optimized.
On the Design and Implementation of a Blockchain-Based Data Management System for ETO Manufacturing
Authors: Jing Zhengjun , Hu Niuping , Song Yurong , Song Bo , Gu Chunsheng , Pan Lei
Published: September 2022

Engineer-to-order (ETO) is a currently popular production model that can meet customers’ individual needs, for which the orders are primarily non-standard parts or small batches. This production model has caused many management challenges, including the difficulty of tracing the production process data of products and the inability to monitor order status in real-time. In this paper, by analyzing the steps of ETO manufacturing and the business process between departments in the manufacturing industry, a blockchain-based process data management system (BPDMS) is proposed. The immutable nature of the blockchain data ensures the data’s validity and consistency in each production step. Furthermore, by embedding the sequential aggregate signature in the system, the sequence verification of discrete process steps can be completed in time. Finally, an electrical equipment assembly production platform is used to discuss the specific implementation on top of the Hyperledger Fabric, a permissioned blockchain. The experiment results show that the proposed system effectively manages the process data of ETO-type production, and the real-time querying of the production status of the orders.
Prediction Model of Dynamic Resilient Modulus of Unsaturated Modified Subgrade under Multi-Factor Combination
Authors: Luan Xiaohan , Han Leilei
Published: September 2022

The objective of this research is to solve the problem of the lack of prediction methods and basis for the long-term road performance of oil shale residue-modified soil in seasonally frozen regions. This paper summarizes and expands the resilient modulus prediction methods in the related literature. Based on the measured soil–water characteristic curve (SWCC) of the compacted modified soil and the trend characteristics of dynamic resilient modulus under freeze–thaw cycles, a semi-empirical prediction model is proposed. This model was used to quantitatively forecast the resilient modulus of unsaturated modified subgrade soil after the freeze–thaw cycle in a seasonal permafrost region. The applicability and accuracy of the method were verified by dynamic resilient modulus tests of the oil shale residue-modified soil under various freeze–thaw cycles and moisture content. The results show that the model has a high degree of fit to the experimental data and is more suitable for predicting the dynamic resilient modulus of modified soil under the change of moisture and the freeze–thaw cycle compared to the existing models.
Characteristics and Hazards of an Aeolian Sand Environment along Railways in the Southeastern Fringe of the Taklimakan Desert and Sand Control Measures
Authors: Ma Benteng , Gao Li , Cheng Jianjun , Ding Bosong , Ding Lusheng , Qu Lei , An Yuanfeng
Published: September 2022

Based on wind velocity and wind direction data monitored by Qiemo and Ruoqiang Meteorological Stations, a systematic elaboration on the wind-sand hazards threatening railways in the study area is given. The results indicate that the study area had an annual sand-moving wind frequency of 7.63–20.09%. The prevailing directions of sand-moving wind were NE and ENE. The annual drift potential (DP) of the study area fell within the range of 66.18–124.21 VU, so the study area had a low-wind-energy environment. The yearly direction variability index fell within 0.594–0.610, which was a medium ratio. The yearly resultant drift directions (RDDs) were 222.34° (SW) and 241.79° (WSW), respectively. The seasonal DPs and sand-moving wind frequencies in various directions manifested consistent variation characteristics. The direction variability index presented obvious seasonal variation characteristics. The surface particles in the study area were primarily extremely fine sand, fine sand, and medium sand, which formed wind-sand flows under the sand-moving wind, resulting in railway erosion and two forms of hazards (sand burial and wind erosion) along railways. Following the “blocking-fixing” principle, sand control measures combining mechanical and biological elements are taken along railways to ensure safe service.
Green Financing Efficiency and Influencing Factors of Chinese Listed Construction Companies against the Background of Carbon Neutralization: A Study Based on Three-Stage DEA and System GMM
Authors: Yu Yaguai , Yan Yina , Shen Panyi , Li Yuting , Ni Taohan
Published: September 2022

This paper combines the green industrial strategy and green financial policies for the construction industry implemented in China in the context of carbon neutrality. A total of 67 listed companies in the construction industry from 2017 to 2020 were taken as the research sample, the green financing efficiency was measured, and its influencing factors were identified based on the three-stage DEA and systematic GMM method. The findings show that the green financing efficiency of listed companies in the construction industry is not high overall, although it is increasing. There are obvious differences in subsectors, among which, the efficiency of architectural design and service industries is relatively high. Overall, the financial environment, and the interaction between the government and the financial market, significantly and positively influence the green financing efficiency. In addition, the macroeconomic environment and the government–enterprise relationship has a complex impact on the green financing efficiency. The ownership concentration and having corporate executives with a financial background have a significant positive impact on the green financing efficiency, and the enterprise size, the debt maturity structure, and the R&D and innovation capability have a significant negative impact. The findings of this paper have implications for the improvement of the policy system that supports green development in the construction industry, and provide guidance for the strategic adjustment of the construction industry itself.
Equivalence between the Existence of Best Proximity Points and Fixed Points for Some Classes of Proximal Contractions
Authors: Som Sumit , Gabeleh Moosa , De la Sen Manuel
Published: September 2022

In the year 2014, Almeida et al. introduced a new class of mappings, namely, contractions of Geraghty type. Additionally, in the year 2021, Beg et al. introduced the concept of generalized F-proximal contraction of the first kind and generalized F-proximal contraction of the second kind, respectively. After developing these concepts, authors mainly studied the best proximity points for these classes of mappings. In this short note, we prove that the problem of the existence of the best proximity points for the said classes of proximal contractions is equivalent to the corresponding fixed points problems.
Computational Techniques Enabling the Perception of Virtual Images Exclusive to the Retinal Afterimage
Authors: de Jong Staas , van der Veer Gerrit
Published: September 2022

The retinal afterimage is a widely known effect in the human visual system, which has been studied and used in the context of a number of major art movements. Therefore, when considering the general role of computation in the visual arts, this begs the question whether this effect, too, may be induced using partly automated techniques. If so, it may become a computationally controllable ingredient of (interactive) visual art, and thus take its place among the many other aspects of visual perception which already have preceded it in this sense. The present moment provides additional inspiration to lay the groundwork for extending computer graphics in general with the retinal afterimage: Historically, we are in a phase where some head-mounted stereoscopic AR/VR technologies are now providing eye tracking by default, thereby allowing realtime monitoring of the processes of visual fixation that can induce the retinal afterimage. A logical starting point for general investigation is then shape display via the retinal afterimage, since shape recognition lends itself well to unambiguous reporting. Shape recognition, however, may also occur due to normal vision, which happens simultaneously. Carefully and rigorously excluding this possibility, we develop computational techniques enabling shape display exclusive to the retinal afterimage.
NMR-Based Metabolomic Analysis for the Effects of Trimethylamine N-Oxide Treatment on C2C12 Myoblasts under Oxidative Stress
Authors: Zou Hong , Huang Caihua , Zhou Lin , Lu Ruohan , Zhang Yimin , Lin Donghai
Published: September 2022

The gut microbial metabolite trimethylamine N-oxide (TMAO) has received increased attention due to its close relationship with cardiovascular disease and type 2 diabetes. In previous studies, TMAO has shown both harmful and beneficial effects on various tissues, but the underlying molecular mechanisms remain to be clarified. Here, we explored the effects of TMAO treatment on H2O2-impaired C2C12 myoblasts, analyzed metabolic changes and identified significantly altered metabolic pathways through nuclear magnetic resonance-based (NMR-based) metabolomic profiling. The results exhibit that TMAO treatment partly alleviated the H2O2-induced oxidative stress damage of cells and protected C2C12 myoblasts by improving cell viability, increasing cellular total superoxide dismutase capacity, improving the protein expression of catalase, and reducing the level of malondialdehyde. We further showed that H2O2 treatment decreased levels of branched-chain amino acids (isoleucine, leucine and valine) and several amino acids including alanine, glycine, threonine, phenylalanine and histidine, and increased the level of phosphocholine related to cell membrane structure, while the TMAO treatment partially reversed the changing trends of these metabolite levels by improving the integrity of the cell membranes. This study indicates that the TMAO treatment may be a promising strategy to alleviate oxidative stress damage in skeletal muscle.
Parental Programming of Offspring Health: The Intricate Interplay between Diet, Environment, Reproduction and Development
Authors: Batra Vipul , Norman Emily , Morgan Hannah L. , Watkins Adam J.
Published: September 2022

As adults, our health can be influenced by a range of lifestyle and environmental factors, increasing the risk for developing a series of non-communicable diseases such as type 2 diabetes, heart disease and obesity. Over the past few decades, our understanding of how our adult health can be shaped by events occurring before birth has developed into a well-supported concept, the Developmental Origins of Health and Disease (DOHaD). Supported by epidemiological data and experimental studies, specific mechanisms have been defined linking environmental perturbations, disrupted fetal and neonatal development and adult ill-health. Originally, such studies focused on the significance of poor maternal health during pregnancy. However, the role of the father in directing the development and well-being of his offspring has come into recent focus. Whereas these studies identify the individual role of each parent in shaping the long-term health of their offspring, few studies have explored the combined influences of both parents on offspring well-being. Such understanding is necessary as parental influences on offspring development extend beyond the direct genetic contributions from the sperm and oocyte. This article reviews our current understanding of the parental contribution to offspring health, exploring some of the mechanisms linking parental well-being with gamete quality, embryo development and offspring health.
The Protective Effect of Simvastatin on the Systolic Function of the Heart in the Model of Acute Ischemia and Reperfusion Is Due to Inhibition of the RhoA Pathway and Independent of Reduction of MMP-2 Activity
Authors: Skrzypiec-Spring Monika , Sapa-Wojciechowska Agnieszka , Rak-Pasikowska Alina , Kaczorowski Maciej , Bil-Lula Iwona , Hałoń Agnieszka , Szeląg Adam
Published: September 2022

The present study investigated whether Rho-associated protein kinase (RhoA/ROCK) signaling pathway inhibitor simvastatin inhibits matrix metalloproteinase 2 (MMP-2) activity in a rat ischemia-reperfusion injury (I/Ri) model by inhibiting the RhoA/ROCK pathway and reducing MMP-2 mRNA levels. Isolated rat hearts were subjected to aerobic perfusion or I/Ri control. The effect of simvastatin was assessed in hearts subjected to I/Ri. We determined cardiac mechanical function, the content of RhoA, phosphorylated myosin light chain subunit 1 (phospho-MYL9), troponin I, MMP-2, and MMP-2 mRNA in the heart homogenates, as well as MMP-2 activity in heart tissue. We showed that treatment with simvastatin caused improvement in the contractile function of the heart subjected to I/Ri which was accompanied by a decrease of MMP-2 activity in heart tissue along with inhibition of RhoA pathway, expressed in a reduction in both RhoA and its downstream product—phosphorylated myosin light chain (phospho-MYL9) in hearts treated with simvastatin. MMP-2 inactivation is not due to inhibition of MMP-2 m-RNA synthesis caused by inhibition of RhoA/ROCK pathway and is due, at least in part, to the direct drug action. The protective effect of simvastatin on systolic function in the acute ischemia-reperfusion model does not appear to be related to reduced MMP-2 activation, but other mechanisms related with the inhibition RhoA/ROCK pathway.
Glutathione-S-Transferases as Potential Targets for Modulation of Nitric Oxide-Mediated Vasodilation
Authors: Russell Tiffany M. , Richardson Des R.
Published: September 2022

Glutathione-S-transferases (GSTs) are highly promiscuous in terms of their interactions with multiple proteins, leading to various functions. In addition to their classical detoxification roles with multi-drug resistance-related protein-1 (MRP1), more recent studies have indicated the role of GSTs in cellular nitric oxide (NO) metabolism. Vasodilation is classically induced by NO through its interaction with soluble guanylate cyclase. The ability of GSTs to biotransform organic nitrates such as nitroglycerin for NO generation can markedly modulate vasodilation, with this effect being prevented by specific GST inhibitors. Recently, other structurally distinct pro-drugs that generate NO via GST-mediated catalysis have been developed as anti-cancer agents and also indicate the potential of GSTs as suitable targets for pharmaceutical development. Further studies investigating GST biochemistry could enhance our understanding of NO metabolism and lead to the generation of novel and innovative vasodilators for clinical use.
Density Functional Theory Study of the Regioselectivity in Copolymerization of bis-Styrenic Molecules with Propylene Using Zirconocene Catalyst
Authors: Yu Shu-Yuan , Peng Xiaoxia , Wang Fuping , Cao Jian , Wang Fei , Zhang Cheng-Gen
Published: September 2022

Density functional theory (DFT) was used to study the regioselectivity of the copolymerization of propylene and the bis-styrenic molecules (DVB and BVPE) using a zirconocene catalyst. This study reveals the following: when hydrogen is introduced to reactivate the catalyst on the vinyl bonds containing DVB or BVPE, the second vinyl bond is inserted into the polymer in a regio-irregular 1,2-way. (I) The 1,2-insertion mode forms more thermodynamically stable products. (II) The 2,1 insertion, DVB-PP1, or BVPE-PP1 needs to rotate 180° along the Zr-C1 bond to complete the process; thus, it is easier to accomplish the 1,2 insertion. (III) The analysis of the local electrophilicity/nucleophilicity index and the Fukui functions also indicate that the 1,2-insertion mode is the optimal insertion mode. Investigating the mechanism of this experimental phenomenon is important in the development of a functionalization strategy for polypropylene (PP) polymers.
Biomaterials and Extracellular Vesicle Delivery: Current Status, Applications and Challenges
Authors: Leung Kasey S. , Shirazi Sajjad , Cooper Lyndon F. , Ravindran Sriram
Published: September 2022

In this review, we will discuss the current status of extracellular vesicle (EV) delivery via biopolymeric scaffolds for therapeutic applications and the challenges associated with the development of these functionalized scaffolds. EVs are cell-derived membranous structures and are involved in many physiological processes. Naïve and engineered EVs have much therapeutic potential, but proper delivery systems are required to prevent non-specific and off-target effects. Targeted and site-specific delivery using polymeric scaffolds can address these limitations. EV delivery with scaffolds has shown improvements in tissue remodeling, wound healing, bone healing, immunomodulation, and vascular performance. Thus, EV delivery via biopolymeric scaffolds is becoming an increasingly popular approach to tissue engineering. Although there are many types of natural and synthetic biopolymers, the overarching goal for many tissue engineers is to utilize biopolymers to restore defects and function as well as support host regeneration. Functionalizing biopolymers by incorporating EVs works toward this goal. Throughout this review, we will characterize extracellular vesicles, examine various biopolymers as a vehicle for EV delivery for therapeutic purposes, potential mechanisms by which EVs exert their effects, EV delivery for tissue repair and immunomodulation, and the challenges associated with the use of EVs in scaffolds.
Implications of a Neuronal Receptor Family, Metabotropic Glutamate Receptors, in Cancer Development and Progression
Authors: Eddy Kevinn , Eddin Mohamad Naser , Fateeva Anna , Pompili Stefano Vito Boccadamo , Shah Raj , Doshi Saurav , Chen Suzie
Published: September 2022

Cancer is the second leading cause of death, and incidences are increasing globally. Simply defined, cancer is the uncontrolled proliferation of a cell, and depending on the tissue of origin, the cancer etiology, biology, progression, prognosis, and treatment will differ. Carcinogenesis and its progression are associated with genetic factors that can either be inherited and/or acquired and are classified as an oncogene or tumor suppressor. Many of these genetic factors converge on common signaling pathway(s), such as the MAPK and PI3K/AKT pathways. In this review, we will focus on the metabotropic glutamate receptor (mGluR) family, an upstream protein that transmits extracellular signals into the cell and has been shown to regulate many aspects of tumor development and progression. We explore the involvement of members of this receptor family in various cancers that include breast cancer, colorectal cancer, glioma, kidney cancer, melanoma, oral cancer, osteosarcoma, pancreatic cancer, prostate cancer, and T-cell cancers. Intriguingly, depending on the member, mGluRs can either be classified as oncogenes or tumor suppressors, although in general most act as an oncogene. The extensive work done to elucidate the role of mGluRs in various cancers suggests that it might be a viable strategy to therapeutically target glutamatergic signaling.
Oil Prices and Exchange Rates: Measurement Matters
Authors: Marquez Jaime
Published: September 2022

This paper examines the relevancy of price measurement for characterizing the relation between real oil prices and real exchange rates. The current empirical literature shows a consensus on using the U.S. CPI to deflate the nominal oil price simply because of its numerous advantages. However, reliance on the U.S. CPI assumes that the worldwide alternative to a barrel of oil is the U.S. consumption basket. There are, however, alternative baskets, and I consider two: the price of gold and the IMF Global Commodity Price Index. Inspection of the results reveals that the relation between real oil prices and real exchange rates is sensitive to the choice of deflator for the price of oil and to the use of effective or bilateral exchange rates. Specifically, using the IMF’s Global Commodity Price Index as a deflator reveals that real oil prices and real exchange rates (effective or bilateral) are clustered along a long-run relation with unitary elasticity. Further, this choice of deflator has the lowest forecast errors. To be sure, much work remains to be completed along the lines of measurement and estimation methods. However, extending the results of this paper will emphasize its main point—namely, that measurement matters.
An Evaluation of Point-of-Care HbA1c, HbA1c Home Kits, and Glucose Management Indicator: Potential Solutions for Telehealth Glycemic Assessments
Authors: Zaharieva Dessi P. , Addala Ananta , Prahalad Priya , Leverenz Brianna , Arrizon-Ruiz Nora , Ding Victoria Y. , Desai Manisha , Karger Amy B. , Maahs David M.
Published: September 2022

During the COVID-19 pandemic, fewer in-person clinic visits resulted in fewer point-of-care (POC) HbA1c measurements. In this sub-study, we assessed the performance of alternative glycemic measures that can be obtained remotely, such as HbA1c home kits and Glucose Management Indicator (GMI) values from Dexcom Clarity. Home kit HbA1c (n = 99), GMI, (n = 88), and POC HbA1c (n = 32) were collected from youth with T1D (age 9.7 ± 4.6 years). Bland–Altman analyses and Lin’s concordance correlation coefficient (𝜌c) were used to characterize the agreement between paired HbA1c measures. Both the HbA1c home kit and GMI showed a slight positive bias (mean difference 0.18% and 0.34%, respectively) and strong concordance with POC HbA1c (𝜌c = 0.982 [0.965, 0.991] and 0.823 [0.686, 0.904], respectively). GMI showed a slight positive bias (mean difference 0.28%) and fair concordance (𝜌c = 0.750 [0.658, 0.820]) to the HbA1c home kit. In conclusion, the strong concordance of GMI and home kits to POC A1c measures suggest their utility in telehealth visits assessments. Although these are not candidates for replacement, these measures can facilitate telehealth visits, particularly in the context of other POC HbA1c measurements from an individual.
Investigating Errors Observed during UAV-Based Vertical Measurements Using Computational Fluid Dynamics
Authors: Hedworth Hayden , Page Jeffrey , Sohl John , Saad Tony
Published: September 2022

Unmanned Aerial Vehicles (UAVs) are a popular platform for air quality measurements. For vertical measurements, rotary-wing UAVs are particularly well-suited. However, an important concern with rotary-wing UAVs is how the rotor-downwash affects measurement accuracy. Measurements from a recent field campaign showed notable discrepancies between data from ascent and descent, which suggested the UAV downwash may be the cause. To investigate and explain these observed discrepancies, we use high-fidelity computational fluid dynamics (CFD) simulations to simulate a UAV during vertical flight. We use a tracer to model a gaseous pollutant and evaluate the impact of the rotor-downwash on the concentration around the UAV. Our results indicate that, when measuring in a gradient, UAV-based measurements were ∼50% greater than the expected concentration during descent, but they were accurate during ascent, regardless of the location of the sensor. These results provide an explanation for errors encountered during vertical measurements and provide insight for accurate data collection methods in future studies.
Authority or Autonomy? Exploring Interactions between Central and Peer Punishments in Risk-Resistant Scenarios
Authors: Qian Jun , Sun Xiao , Zhang Tongda , Chai Yueting
Published: September 2022

Game theory provides a powerful means to study human cooperation and better understand cooperation-facilitating mechanisms in general. In classical game-theoretic models, an increase in group cooperation constantly increases people’s gains, implying that individual gains are a continuously varying function of the cooperation rate. However, this is inconsistent with the increasing number of risk-resistant scenarios in reality. A risk-resistant scenario means once a group does not successfully resist the risk, all individuals lose their resources, such as a community coping with COVID-19 and a village resisting a flood. In other words, individuals’ gains are segmented about the collaboration rate. This paper builds a risk-resistant model to explore whether punishment still promotes collaboration when people resist risk. The results show that central and peer punishments can both encourage collaboration but with different characteristics under different risk-resistant scenarios. Specifically, central punishment constrains the collaboration motivated by peer punishment regardless of risk, while peer punishment limits the collaboration induced by central punishment only when the risk is high. Our findings provide insights into the balance between peer punishment from public autonomy and central punishment from central governance, and the proposed model paves the way for the development of richer risk-resistant models.
Multifidelity Model Calibration in Structural Dynamics Using Stochastic Variational Inference on Manifolds
Authors: Tsilifis Panagiotis , Pandita Piyush , Ghosh Sayan , Wang Liping
Published: September 2022

Bayesian techniques for engineering problems, which rely on Gaussian process (GP) regression, are known for their ability to quantify epistemic and aleatory uncertainties and for being data efficient. The mathematical elegance of applying these methods usually comes at a high computational cost when compared to deterministic and empirical Bayesian methods. Furthermore, using these methods becomes practically infeasible in scenarios characterized by a large number of inputs and thousands of training data. The focus of this work is on enhancing Gaussian process based metamodeling and model calibration tasks, when the size of the training datasets is significantly large. To achieve this goal, we employ a stochastic variational inference algorithm that enables rapid statistical learning of the calibration parameters and hyperparameter tuning, while retaining the rigor of Bayesian inference. The numerical performance of the algorithm is demonstrated on multiple metamodeling and model calibration problems with thousands of training data.
Freshwater Invertebrate Assemblage Composition and Water Quality Assessment of an Urban Coastal Watershed in the Context of Land-Use Land-Cover and Reach-Scale Physical Habitat
Authors: Henderson Nicole D. , Christian Alan D.
Published: September 2022

Stream ecosystems provide invaluable ecosystem services but are highly impacted ecosystems in need of water quality monitoring for habitat change impacts. Freshwater macroinvertebrate (FWI) assemblages have been shown to be good indicators of water quality and are known to be vulnerable to land-use land cover (LULC) and other habitat changes. The goal of this case study was to use an existing dominant LULC analysis in the Neponset River watershed, Massachusetts, USA, as LULC sampling treatment groups to deliberately capture the influence of these LULC effects on meso-scale habitat quality, FWI assemblages, and FWI water quality indices at eight sampling reaches. To achieve this goal, we collected physical habitat measurements and FWI samples in the summers of 2010 and 2012 at eight reach-scale stations spread across four previously determined LULC sub-watershed types (forest, residential, industrial, and golf) in the watershed. We expected that LULC change would influence the habitat quality, which would influence the FWI assemblage water quality scores and composition. We also expected that the water quality at these LULC sub-watershed types would be reflected in the FWI assemblage composition. We identified five major findings from our study. Our first finding was that the habitat quality in the Neponset River watershed was somewhat degraded relative to pristine conditions. Our second finding was that our habitat characterization analysis reflected some separation of our reach-scale macrohabitat types at land-use land-cover treatment stations with some correlations with microhabitat variables. Our third finding was that the water quality base on FWI assemblages was generally degraded in reference to pristine conditions. Our fourth finding was that, contrary to our expectations, there was no significant correlation between our reach-scale EPA habitat quality scores and FWI water quality scores. Our fifth finding was that our FWI assemblage NMS showed separation of land-use land-cover sampling stations and that that low pollution-tolerant taxa dominated some of our LULC sampling treatment stations and influenced NMS groupings.