bluuebunny/crossref_metadata_2025 huggingface dataset:
A Novel Electrochemical Sensor Based on an Environmentally Friendly Synthesis of Magnetic Chitosan Nanocomposite Carbon Paste Electrode for the Determination of Diclofenac to Control Inflammation
Authors: Abd-Elsabour Mohamed , Abou-Krisha Mortaga M. , Kenawy Sayed H. , Yousef Tarek A.
Published: March 2023

A simple and eco-friendly electrochemical sensor for the anti-inflammatory diclofenac (DIC) was developed in a chitosan nanocomposite carbon paste electrode (M-Chs NC/CPE). The M-Chs NC/CPE was characterized with FTIR, XRD, SEM, and TEM for the size, surface area, and morphology. The produced electrode showed a high electrocatalytic activity to use the DIC in 0.1 M of the BR buffer (pH 3.0). The effect of scanning speed and pH on the DIC oxidation peak suggests that the DIC electrode process has a typical diffusion characteristic with two electrons and two protons. Furthermore, the peak current linearly proportional to the DIC concentration ranged from 0.025 M to 4.0 M with the correlation coefficient (r2). The sensitivity, limit of detection (LOD; 3σ), and the limit of quantification (LOQ; 10σ) were 0.993, 9.6 µA/µM cm2, 0.007 µM, and 0.024 µM, respectively. In the end, the proposed sensor enables the reliable and sensitive detection of DIC in biological and pharmaceutical samples.
Plasma-Etched Vertically Aligned CNTs with Enhanced Antibacterial Power
Authors: Schifano Emily , Cavoto Gianluca , Pandolfi Francesco , Pettinari Giorgio , Apponi Alice , Ruocco Alessandro , Uccelletti Daniela , Rago Ilaria
Published: March 2023

The emergence of multidrug-resistant bacteria represents a growing threat to public health, and it calls for the development of alternative antibacterial approaches not based on antibiotics. Here, we propose vertically aligned carbon nanotubes (VA-CNTs), with a properly designed nanomorphology, as effective platforms to kill bacteria. We show, via a combination of microscopic and spectroscopic techniques, the ability to tailor the topography of VA-CNTs, in a controlled and time-efficient manner, by means of plasma etching processes. Three different varieties of VA-CNTs were investigated, in terms of antibacterial and antibiofilm activity, against Pseudomonas aeruginosa and Staphylococcus aureus: one as-grown variety and two varieties receiving different etching treatments. The highest reduction in cell viability (100% and 97% for P. aeruginosa and S. aureus, respectively) was observed for the VA-CNTs modified using Ar and O2 as an etching gas, thus identifying the best configuration for a VA-CNT-based surface to inactivate both planktonic and biofilm infections. Additionally, we demonstrate that the powerful antibacterial activity of VA-CNTs is determined by a synergistic effect of both mechanical injuries and ROS production. The possibility of achieving a bacterial inactivation close to 100%, by modulating the physico-chemical features of VA-CNTs, opens up new opportunities for the design of self-cleaning surfaces, preventing the formation of microbial colonies.
The Role of Diet as a Modulator of the Inflammatory Process in the Neurological Diseases
Authors: Kurowska Antonina , Ziemichód Wojciech , Herbet Mariola , Piątkowska-Chmiel Iwona
Published: March 2023

Neurological diseases are recognized as major causes of disability and mortality worldwide. Due to the dynamic progress of diseases such as Alzheimer’s disease (AD), Parkinson’s Disease (PD), Schizophrenia, Depression, and Multiple Sclerosis (MD), scientists are mobilized to look for new and more effective methods of interventions. A growing body of evidence suggests that inflammatory processes and an imbalance in the composition and function of the gut microbiome, which play a critical role in the pathogenesis of various neurological diseases and dietary interventions, such as the Mediterranean diet the DASH diet, or the ketogenic diet can have beneficial effects on their course. The aim of this review was to take a closer look at the role of diet and its ingredients in modulating inflammation associated with the development and/or progression of central nervous system diseases. Presented data shows that consuming a diet abundant in fruits, vegetables, nuts, herbs, spices, and legumes that are sources of anti-inflammatory elements such as omega-3 fatty acids, polyphenols, vitamins, essential minerals, and probiotics while avoiding foods that promote inflammation, create a positive brain environment and is associated with a reduced risk of neurological diseases. Personalized nutritional interventions may constitute a non-invasive and effective strategy in combating neurological disorders.
Green Tea and Benign Gynecologic Disorders: A New Trick for An Old Beverage?
Authors: Hazimeh Dana , Massoud Gaelle , Parish Maclaine , Singh Bhuchitra , Segars James , Islam Md Soriful
Published: March 2023

Green tea is harvested from the tea plant Camellia sinensis and is one of the most widely consumed beverages worldwide. It is richer in antioxidants than other forms of tea and has a uniquely high content of polyphenolic compounds known as catechins. Epigallocatechin-3-gallate (EGCG), the major green tea catechin, has been studied for its potential therapeutic role in many disease contexts, including pathologies of the female reproductive system. As both a prooxidant and antioxidant, EGCG can modulate many cellular pathways important to disease pathogenesis and thus has clinical benefits. This review provides a synopsis of the current knowledge on the beneficial effects of green tea in benign gynecological disorders. Green tea alleviates symptom severity in uterine fibroids and improves endometriosis through anti-fibrotic, anti-angiogenic, and pro-apoptotic mechanisms. Additionally, it can reduce uterine contractility and improve the generalized hyperalgesia associated with dysmenorrhea and adenomyosis. Although its role in infertility is controversial, EGCG can be used as a symptomatic treatment for menopause, where it decreases weight gain and osteoporosis, as well as for polycystic ovary syndrome (PCOS).
Human Milk Oligosaccharides Variation in Gestational Diabetes Mellitus Mothers
Authors: Dou Yuqi , Luo Yuanli , Xing Yan , Liu Hui , Chen Botian , Zhu Liye , Ma Defu , Zhu Jing
Published: March 2023

Gestational diabetes mellitus (GDM) is a common disease of pregnancy, but with very limited knowledge of its impact on human milk oligosaccharides (HMOs) in breast milk. This study aimed to explore the lactational changes in the concentration of HMOs in exclusively breastfeeding GDM mothers and the differences between GDM and healthy mothers. A total of 22 mothers (11 GDM mothers vs. 11 healthy mothers) and their offspring were enrolled in the study and the levels of 14 HMOs were measured in colostrum, transitional milk, and mature milk. Most of the HMOs showed a significant temporal trend with decreasing levels over lactation; however, there were some exceptions for 2′-Fucosyllactose (2′-FL), 3-Fucosyllactose (3-FL), Lacto-N-fucopentaose II (LNFP-II), and Lacto-N-fucopentaose III (LNFP-III). Lacto-N-neotetraose (LNnT) was significantly higher in GDM mothers in all time points and its concentrations in colostrum and transitional milk were correlated positively with the infant’s weight-for-age Z-score at six months postnatal in the GDM group. Significant group differences were also found in LNFP-II, 3′-Sialyllactose (3′-SL), and Disialyllacto-N-tetraose (DSLNT) but not in all lactational periods. The role of differently expressed HMOs in GDM needs to be further explored by follow-up studies.
Vegan Diet Is Associated with a Lower Risk of Chronic Kidney Disease in Patients with Hyperuricemia
Authors: Wu Chia-Lin , Tsai Wen-Hsin , Liu Jia-Sin , Liu Hao-Wen , Huang Sin-Yi , Kuo Ko-Lin
Published: March 2023

Hyperuricemia is a well-known risk factor for chronic kidney disease (CKD). Little is known about whether a vegetarian diet is associated with a lower risk of CKD in patients with hyperuricemia. From 5 September 2005, to 31 December 2016, we retrospectively included clinically stable patients with hyperuricemia who received health check-ups at Taipei Tzu Chi Hospital. All participants completed a dietary habits questionnaire to determine whether they were omnivorous, lacto-ovo vegetarian, or vegan. CKD was defined as an estimated glomerular filtration rate <60 mL/min/1.73 m2 or the presence of proteinuria. A total of 3618 patients with hyperuricemia were recruited for this cross-sectional study, consisting of 225 vegans, 509 lacto-ovo vegetarians, and 2884 omnivores. After adjusting for age and sex, vegans had a significantly lower odds ratio (OR) of CKD than omnivores (OR, 0.62; p = 0.006). The OR of CKD remained significantly lower in vegans after adjusting for additional confounders (OR, 0.69; p = 0.04). Additionally, age (per year OR, 1.06; p < 0.001), diabetes mellitus (OR, 2.12; p < 0.001), hypertension (OR, 1.73; p < 0.001), obesity (OR, 1.24; p = 0.02), smoking (OR, 2.05; p < 0.001), and very high uric acid levels (OR, 2.08; p < 0.001) were independent risk factors for CKD in patients with hyperuricemia. Moreover, structural equation modeling revealed that a vegan diet was associated with a lower OR of CKD (OR, 0.69; p < 0.05). A vegan diet is associated with a 31% lower risk of CKD in patients with hyperuricemia. A vegan diet may be beneficial in reducing the occurrence of CKD in patients with hyperuricemia.
Delivering Food Resources and Kitchen Skills (FoRKS) to Adults with Food Insecurity and Hypertension: A Pilot Study
Authors: Rivera Rebecca L. , Adams Mariah , Dawkins Emily , Carter Amy , Zhang Xuan , Tu Wanzhu , Peña Armando , Holden Richard J. , Clark Daniel O.
Published: March 2023

Food insecurity affects nearly 50 million Americans and is linked to cardiovascular disease risk factors and health disparities. The purpose of this single-arm pilot study was to determine the feasibility of a 16-week dietitian-led lifestyle intervention to concurrently address food access, nutrition literacy, cooking skills, and hypertension among safety-net primary care adult patients. The Food Resources and Kitchen Skills (FoRKS) intervention provided nutrition education and support for hypertension self-management, group kitchen skills and cooking classes from a health center teaching kitchen, medically tailored home-delivered meals and meal kits, and a kitchen toolkit. Feasibility and process measures included class attendance rates and satisfaction and social support and self-efficacy toward healthy food behaviors. Outcome measures included food security, blood pressure, diet quality, and weight. Participants (n = 13) were on average {mean (SD)} aged 58.9 ± 4.5 years, 10 were female, and 12 were Black or African American. Attendance averaged 19 of 22 (87.1%) classes and satisfaction was rated as high. Food self-efficacy and food security improved, and blood pressure and weight declined. FoRKS is a promising intervention that warrants further evaluation for its potential to reduce cardiovascular disease risk factors among adults with food insecurity and hypertension.
The Link between Iron Turnover and Pharmacotherapy in Transplant Patients
Authors: Delijewski Marcin , Bartoń Aleksandra , Maksym Beata , Pawlas Natalia
Published: March 2023

Iron is a transition metal that plays a crucial role in several physiological processes. It can also exhibit toxic effects on cells, due to its role in the formation of free radicals. Iron deficiency and anemia, as well as iron overload, are the result of impaired iron metabolism, in which a number of proteins, such as hepcidin, hemojuvelin and transferrin, take part. Iron deficiency is common in individuals with renal and cardiac transplants, while iron overload is more common in patients with hepatic transplantation. The current knowledge about iron metabolism in lung graft recipients and donors is limited. The problem is even more complex when we consider the fact that iron metabolism may be also driven by certain drugs used by graft recipients and donors. In this work, we overview the available literature reports on iron turnover in the human body, with particular emphasis on transplant patients, and we also attempt to assess the drugs’ impact on iron metabolism, which may be useful in perioperative treatment in transplantology.
Light-Nuclei Production in Heavy-Ion Collisions at sNN = 6.4 – 19.6 GeV in THESEUS Generator Based on Three-Fluid Dynamics
Authors: Kozhevnikova Marina , Ivanov Yuri B.
Published: March 2023

Light-nuclei production in relativistic heavy-ion collisions is simulated within an updated Three-fluid Hydrodynamics-based Event Simulator Extended by UrQMD (Ultra-relativistic Quantum Molecular Dynamics) final State interactions (THESEUS). The simulations are performed in the collision energy range of sNN= 6.4–19.6 GeV. The light-nuclei are produced within the thermodynamical approach on an equal basis with hadrons. Since the light nuclei do not participate in the UrQMD evolution, the only additional parameter related to the light nuclei, i.e., the energy density of late freeze-out, is used for the imitation of the afterburner stage of the collision. The updated THESEUS provides a reasonable reproduction of data on bulk observables of the light nuclei, especially their functional dependence on the collision energy and light-nucleus mass. Various ratios, d/p, t/p, t/d, and N(t)×N(p)/N2(d), are also considered. Imperfect reproduction of the light-nuclei data leaves room for medium effects in produced light nuclei.
A Systematic Review of In Vivo Studies of the Efficacy of Herbal Medicines for Anti-Aging in the Last Five Years
Authors: Cho Seung-Yeon , Lee Han-Gyul , Kwon Seungwon , Park Seong-Uk , Jung Woo-Sang , Moon Sang-Kwan , Park Jung-Mi , Ko Chang-Nam
Published: March 2023

Background: The world’s population is rapidly aging, and attention to and research on the increase in life expectancy and age-related diseases are needed. This study aimed to review the in vivo studies on the anti-aging effects of herbal medicines. Methods: In vivo studies of single or complex herbal medicines for anti-aging that were published in the last five years were included in this review. The following databases were used: PubMed, Scopus, ScienceDirect, Web of Science and EMBASE. Results: A total of 41 studies were considered eligible for the review. The articles were classified into body organs and functions, experimental country, herbal medicine, extraction method, administration route, dosage, duration, animal model, aging-induced method, sex, number of animals per group, and outcomes and mechanisms A single herbal extract was used in a total of 21 studies including Alpinia oxyphylla Miq., Acanthopanax senticosus and Lyceum barbarum, and a multi-compound herbal prescription was used in a total of 20 studies, including Modified Qiongyu paste, Wuzi Yanzong recipe, etc. Each herbal medicine had anti-aging effects on learning and memory, cognition, emotion, internal organs, gastrointestinal tracts, sexual functions, musculoskeletal function and so on. The common mechanisms of action were antioxidant and anti-inflammatory, and various effects and mechanisms for each organ and function were identified. Conclusions: Herbal medicine exhibited beneficial effects on anti-aging in various parts of the body and its function. Further investigation of the appropriate herbal medicine prescriptions and their components is recommended.
A New Strategy for Nucleic Acid Delivery and Protein Expression Using Biocompatible Nanohydrogels of Predefined Sizes
Authors: Eswaran Lakshmanan , Kazimirsky Gila , Yehuda Ronen , Byk Gerardo
Published: March 2023

We have developed new formulations of nanohydrogels (NHGs) complexed with DNA devoid of cell toxicity, which, together with their tuned sizes, makes them of great interest for delivering DNA/RNA for foreign protein expression. Transfection results demonstrate that, unlike classical lipo/polyplexes, the new NHGs can be incubated indefinitely with cells without apparent cellular toxicity, resulting in the high expression of foreign proteins for long periods of time. Although protein expression starts with a delay as compared to classical systems, it is sustained for a long period of time, even after passing cells without observation of toxicity. A fluorescently labelled NHG used for gene delivery was detected inside cells very early after incubation, but the protein expression was delayed by many days, demonstrating that there is a time-dependent release of genes from the NHGs. We suggest that this delay is due to the slow but continuous release of DNA from the particles concomitantly with slow but continuous protein expression. Additionally, results obtained after the in vivo administration of m-Cherry/NHG complexes indicated a delayed but prolonged expression of the marker gene in the tissue of administration. Overall, we have demonstrated gene delivery and foreign protein expression using GFP and m-Cherry marker genes complexed with biocompatible nanohydrogels.
SGLT2 Inhibitor—Dapagliflozin Attenuates Diabetes-Induced Renal Injury by Regulating Inflammation through a CYP4A/20-HETE Signaling Mechanism
Authors: Dia Batoul , Alkhansa Sahar , Njeim Rachel , Al Moussawi Sarah , Farhat Theresa , Haddad Antony , Riachi Mansour E. , Nawfal Rashad , Azar William S. , Eid Assaad A.
Published: March 2023

Diabetic kidney disease (DKD) is a serious complication of diabetes, affecting millions of people worldwide. Inflammation and oxidative stress are key contributors to the development and progression of DKD, making them potential targets for therapeutic interventions. Sodium-glucose cotransporter 2 inhibitors (SGLT2i) have emerged as a promising class of drugs, with evidence demonstrating that they can improve renal outcomes in people with diabetes. However, the exact mechanism by which SGLT2i exert their renoprotective effects is not yet fully understood. This study demonstrates that dapagliflozin treatment attenuates renal injury observed in type 2 diabetic mice. This is evidenced by the reduction in renal hypertrophy and proteinuria. Furthermore, dapagliflozin decreases tubulointerstitial fibrosis and glomerulosclerosis by mitigating the generation of reactive oxygen species and inflammation, which are activated through the production of CYP4A-induced 20-HETE. Our findings provide insights onto a novel mechanistic pathway by which SGLT2i exerts their renoprotective effects. Overall, and to our knowledge, the study provides critical insights into the pathophysiology of DKD and represents an important step towards improving outcomes for people with this devastating condition.
Antimicrobial Nano-Zinc Oxide Biocomposites for Wound Healing Applications: A Review
Authors: Pino Paolo , Bosco Francesca , Mollea Chiara , Onida Barbara
Published: March 2023

Chronic wounds are a major concern for global health, affecting millions of individuals worldwide. As their occurrence is correlated with age and age-related comorbidities, their incidence in the population is set to increase in the forthcoming years. This burden is further worsened by the rise of antimicrobial resistance (AMR), which causes wound infections that are increasingly hard to treat with current antibiotics. Antimicrobial bionanocomposites are an emerging class of materials that combine the biocompatibility and tissue-mimicking properties of biomacromolecules with the antimicrobial activity of metal or metal oxide nanoparticles. Among these nanostructured agents, zinc oxide (ZnO) is one of the most promising for its microbicidal effects and its anti-inflammatory properties, and as a source of essential zinc ions. This review analyses the most recent developments in the field of nano-ZnO–bionanocomposite (nZnO-BNC) materials—mainly in the form of films, but also hydrogel or electrospun bandages—from the different preparation techniques to their properties and antibacterial and wound-healing performances. The effect of nanostructured ZnO on the mechanical, water and gas barrier, swelling, optical, thermal, water affinity, and drug-release properties are examined and linked to the preparation methods. Antimicrobial assays over a wide range of bacterial strains are extensively surveyed, and wound-healing studies are finally considered to provide a comprehensive assessment framework. While early results are promising, a systematic and standardised testing procedure for the comparison of antibacterial properties is still lacking, partly because of a not-yet fully understood antimicrobial mechanism. This work, therefore, allowed, on one hand, the determination of the best strategies for the design, engineering, and application of n-ZnO-BNC, and, on the other hand, the identification of the current challenges and opportunities for future research.
Plant-Derived Extracellular Vesicles as a Delivery Platform for RNA-Based Vaccine: Feasibility Study of an Oral and Intranasal SARS-CoV-2 Vaccine
Authors: Pomatto Margherita A. C. , Gai Chiara , Negro Federica , Massari Lucia , Deregibus Maria Chiara , Grange Cristina , De Rosa Francesco Giuseppe , Camussi Giovanni
Published: March 2023

Plant-derived extracellular vesicles (EVs) may represent a platform for the delivery of RNA-based vaccines, exploiting their natural membrane envelope to protect and deliver nucleic acids. Here, EVs extracted from orange (Citrus sinensis) juice (oEVs) were investigated as carriers for oral and intranasal SARS-CoV-2 mRNA vaccine. oEVs were efficiently loaded with different mRNA molecules (coding N, subunit 1 and full S proteins) and the mRNA was protected from degrading stress (including RNase and simulated gastric fluid), delivered to target cells and translated into protein. APC cells stimulated with oEVs loaded with mRNAs induced T lymphocyte activation in vitro. The immunization of mice with oEVs loaded with S1 mRNA via different routes of administration including intramuscular, oral and intranasal stimulated a humoral immune response with production of specific IgM and IgG blocking antibodies and a T cell immune response, as suggested by IFN-γ production by spleen lymphocytes stimulated with S peptide. Oral and intranasal administration also triggered the production of specific IgA, the mucosal barrier in the adaptive immune response. In conclusion, plant-derived EVs represent a useful platform for mRNA-based vaccines administered not only parentally but also orally and intranasally.
Sodium-Glucose Cotransporter-2 (SGLT-2) Inhibitors Use among Heart Failure Patients and the Role of Pharmacists in Early Initiation of Therapy
Authors: Aldhaeefi Mohammed , Beers Brandon , Shah Jenny , Rizi Saba Saeidi , Rungkitwattanakul Dhakrit , Nimoh Oliver , Frimpong Victoria , Gonzalez Jackie , Belrhiti Sanaa , Urooj Fatima , Williams Deborah
Published: March 2023

Heart failure (HF) is a growing major public health and economic concern in the United States and worldwide. Heart failure mortality rates can be as high as 75% despite advances in therapies. HF is expected to be the fastest growing among all cardiovascular diseases, with HF-associated direct medical costs projected to nearly double over the next 10 years. Hospital admissions, re-admission, and medical cost are a huge burden to the healthcare system, and this is estimated to have increased gradually over the past decades despite the available advances in HF treatment and prevention. Many heart failure therapies have shown improvement in terms of mortality, morbidity, and symptomatic management. Guideline-directed medical therapy (GDMT) for heart failure has proven its ability to reduce morbidity and mortality by 66%. GDMT is recommended to be used among all HF patients when appropriate. In recent years, two new drug classes, angiotensin receptor-neprilysin inhibitor (ARNi) and sodium-glucose cotransporter-2 (SGLT-2) inhibitors, were approved by the United States Food and Drug Administration (US FDA) for the management of heart failure. The exact mechanism by which the SGLT-2 inhibitors attenuate the inflammatory process remains unclear. Several mechanisms have been suggested related to the cardiovascular benefit of SGLT-2 inhibitors, including a reduction in inflammation, improvement in natriuresis/diuresis, and promotion of the use of ketones as a secondary energy source. Clinical data showed that SGLT-2 inhibitors have morbidity and mortality benefits within 30 days of initiation. Studies have proven that clinical pharmacists practicing in HF inpatient and outpatient settings resulted in a reduction of HF hospitalization and an increase in the uptake of GDMT by initiating or up-titrating GDMT agents as well as providing patient education.
Three-Dimensional Mapping Technology for Structural Deformation during Aircraft Assembly Process
Authors: Liu Yue , Yan Dongming , Li Lijuan , Lin Xuezhu , Guo Lili
Published: March 2023

Owing to the assembly state changes during aircraft assembly processes, assembly force-deformation problem occurs. To obtain the structure shape in the product assembly process efficiently and accurately, a three-dimensional (3D) mapping technology for the structural deformation during the aircraft assembly process is proposed combined with a fiber Bragg grating (FBG) optical fiber sensor and binocular vision measurement system. First, this study established a curvature transformation model using optical fiber monitoring data, obtained the 3D spatial deformation of the product, and completed the unification of the optical fiber wavelength change and spatial 3D point coordinate heterogeneous data. Second, a mesh deformation optimization algorithm based on point-cloud optimization was established. Subsequently, the deformation effects of four mesh deformation models were compared to verify the feasibility and accuracy of HEC-Laplace, and the 3D mapping of the product structure shape in the assembly process was realized. Finally, a cantilever wing model was used to verify the deformation of different loading modes. The results show that the product structure changes can be accurately obtained through the proposed technology, thereby improving the accuracy control and overall assembly quality in the aircraft assembly process and providing a theoretical basis for intelligent aircraft assembly.
Top Canopy Height and Stem Size Variation Enhance Aboveground Biomass across Spatial Scales in Seasonal Tropical Forests
Authors: Sun Zhenhua , Sonsuthi Arunkamon , Jucker Tommaso , Ali Arshad , Cao Min , Liu Feng , Cao Guanghong , Hu Tianyu , Ma Qin , Guo Qinghua , Lin Luxiang
Published: March 2023

Tropical forests are biologically diverse and structurally complex ecosystems that can store a large quantity of carbon and support a great variety of plant and animal species. However, tropical forest structure can vary dramatically within seemingly homogeneous landscapes due to subtle changes in topography, soil fertility, species composition and past disturbances. Although numerous studies have reported the effects of field-based stand structure attributes on aboveground biomass (AGB) in tropical forests, the relative effects and contributions of UAV LiDAR-based canopy structure and ground-based stand structural attributes in shaping AGB remain unclear. Here, we hypothesize that mean top-of-canopy height (TCH) enhances AGB directly and indirectly via species richness and horizontal stand structural attributes, but these positive relationships are stronger at a larger spatial scale. We used a combined approach of field inventory and LiDAR-based remote sensing to explore how stand structural attributes (stem abundance, size variation and TCH) and tree species richness affect AGB along an elevational gradient in tropical forests at two spatial scales, i.e., 20 m × 20 m (small scale), and 50 m × 50 m (large scale) in southwest China. Specifically, we used structural equation models to test the proposed hypothesis. We found that TCH, stem size variation and abundance were strongly positively associated with AGB at both spatial scales, in addition to which increasing TCH led to greater AGB indirectly through increased stem size variation. Species richness had negative to negligible influences on AGB, but species richness increased with increasing stem abundance at both spatial scales. Our results suggest that light capture and use, modulated by stand structure, are key to promoting high AGB stocks in tropical forests. Thus, we argue that both horizontal and vertical stand structures are important for shaping AGB, but the relative contributions vary across spatial scales in tropical forests. Importantly, our results highlight the importance of including vertical forest stand attributes for predicting AGB and carbon sequestration that underpins human wellbeing.
Chemical Composition and Biological Activities of Essential Oils from Origanum vulgare Genotypes Belonging to the Carvacrol and Thymol Chemotypes
Authors: Zinno Paola , Guantario Barbara , Lombardi Gabriele , Ranaldi Giulia , Finamore Alberto , Allegra Sofia , Mammano Michele Massimo , Fascella Giancarlo , Raffo Antonio , Roselli Marianna
Published: March 2023

The remarkable biological activities of oregano essential oils (EOs) have recently prompted a host of studies aimed at exploring their potential innovative applications in the food and pharmaceutical industries. The chemical composition and biological activities of EOs from two Origanum vulgare genotypes, widely cultivated in Sicily and not previously studied for their biological properties, were characterized. Plants of the two genotypes, belonging to the carvacrol (CAR) and thymol (THY) chemotypes and grown in different cultivation environments, were considered for this study. The chemical profiles, including the determination of enantiomeric distribution, of the EOs, obtained by hydrodistillation from dried leaves and flowers, were investigated by GC–MS. Biological activity was evaluated as antimicrobial properties against different pathogen indicator strains, while intestinal barrier integrity, reduction in pathogen adhesion and anti-inflammatory actions were assayed in the intestinal Caco-2 cell line. The chemical profile of the CAR genotype was less complex and characterized by higher levels of the most active compound, i.e., carvacrol, when compared to the THY genotype. The enantiomeric distribution of chiral constituents did not vary across genotypes, while being markedly different from that observed in Origanum vulgare genotypes from other geographical origins. In general, all EOs showed high antimicrobial activity, both in vitro and in a food matrix challenge test. Representative EOs from the two genotypes resulted not altering epithelial monolayer sealing only for concentrations lower than 0.02%, were able to reduce the adhesion of selected pathogens, but did not exert relevant anti-inflammatory effects. These results suggest their potential use as control agents against a wide spectrum of foodborne pathogens.
Quality Changes of Cold-Pressed Black Cumin (Nigella sativa L.), Safflower (Carthamus tinctorius L.), and Milk Thistle (Silybum marianum L.) Seed Oils during Storage
Authors: Tarasevičienė Živilė , Laukagalis Valdas , Paulauskienė Aurelija , Baltušnikienė Aldona , Meškinytė Edita
Published: March 2023

Oils derived from non-traditional seeds, such as safflower, milk thistle, and black cumin seeds, have recently grown in popularity. Seed oil is in high demand due to consumer interest in illness prevention and health promotion through healthier diets that include a high concentration of monounsaturated and polyunsaturated fatty acids and antioxidant phenolic components. This study assessed the quality characteristics of cold-pressed seed oil at three unique storage times: at the beginning of the trial (i.e., before storage), after 2 months, and after 4 months. The results of the performed analyses indicate that the acidity of extracted black cumin, safflower, and milk thistle seed oil fluctuates considerably over time. The highest acidity level change was detected for black cumin seed oil, from 10.26% after the extraction to 16.96% after 4 months of storage at 4 °C. Consequently, changes between pre- and post-storage peroxide concentrations were discernible after four months. Peroxide value in milk thistle and safflower seed oils increased by 0.92 meq/kg and 2.00 meq/kg, respectively, during the assessed storage time, while that of black cumin was very high and fluctuated. The storage period substantially affects oxidative changes and the oxidation stability of the oil. Major changes were observed in the polyunsaturated fatty acids in seed oil during storage. The essential changes were detected in the black cumin seed oil odor profile after 4 storage months. Their quality and stability, as well as the nature of the changes that occur during the storage of oil, require extensive investigation.
Polysaccharides as Effective and Environmentally Friendly Inhibitors of Scale Deposition from Aqueous Solutions in Technological Processes
Authors: Fakhreeva Alsu Venerovna , Nosov Vasily Viktorovich , Voloshin Alexander Iosifovich , Dokichev Vladimir Anatolyevich
Published: March 2023

In this paper, we consider natural and modified polysaccharides for use as active ingredients in scale deposition inhibitors to prevent the formation of scale in oil production equipment, heat exchange equipment, and water supply systems. Modified and functionalized polysaccharides with a strong ability to inhibit the formation of deposits of typical scale, such as carbonates and sulfates of alkaline earth elements found in technological processes, are described. This review discusses the mechanisms of the inhibition of crystallization using polysaccharides, and the various methodological aspects of evaluating their effectiveness are considered. This review also provides information on the technological application of scale deposition inhibitors based on polysaccharides. Special attention is paid to the environmental aspect of the use of polysaccharides in industry as scale deposition inhibitors.
Novel 1,2-Bismethacrylate-3-Eugenyl Propane for Resin Composites: Synthesis, Characterization, Rheological, and Degree of Conversion
Authors: Al-Kahtani Haifa Masfeer , Al-Odayni Abdel-Basit , Saeed Waseem Sharaf , Robaian Ali , Al-Kahtani Abdullah , Aouak Taieb , Alrahlah Ali
Published: March 2023

This work aimed to synthesize a novel dimethacrylated-derivative of eugenol (Eg) (termed EgGAA) as potential biomaterial for certain applications such as dental fillings and adhesives. EgGAA was synthesized through a two-step reaction: (i) a mono methacrylated-eugenol (EgGMA) was produced via a ring-opening etherification of glycidyl methacrylate (GMA) with Eg; (ii) EgGMA was condensed with methacryloyl chloride into EgGAA. EgGAA was further incorporated in matrices containing BisGMA and TEGDMA (50:50 wt%) (TBEa), in which EgGAA replaced BisGMA as 0–100 wt% to get a series of unfilled resin composites (TBEa0–TBEa100), and by addition of reinforcing silica (66 wt%), a series of filled resins were also obtained (F-TBEa0–F-TBEa100). Synthesized monomers were analyzed for their structural, spectral, and thermal properties using FTIR, 1H- and 13C-NMR, mass spectrometry, TGA, and DSC. Composites rheological and DC were analyzed. The viscosity (η, Pa·s) of EgGAA (0.379) was 1533 times lower than BisGMA (581.0) and 125 times higher than TEGDMA (0.003). Rheology of unfilled resins (TBEa) indicated Newtonian fluids, with viscosity decreased from 0.164 Pa·s (TBEa0) to 0.010 Pa·s (TBEa100) when EgGAA totally replaced BisGMA. However, composites showed non-Newtonian and shear-thinning behavior, with complex viscosity (η*) being shear-independent at high angular frequencies (10–100 rad/s). The loss factor crossover points were at 45.6, 20.3, 20.4, and 25.6 rad/s, indicating a higher elastic portion for EgGAA-free composite. The DC was insignificantly decreased from 61.22% for the control to 59.85% and 59.50% for F-TBEa25 and F-TBEa50, respectively, while the difference became significant when EgGAA totally replaced BisGMA (F-TBEa100, DC = 52.54%). Accordingly, these properties could encourage further investigation of Eg-containing resin-based composite as filling materials in terms of their physicochemical, mechanical, and biological potentiality as dental material.
The β Form in PVDF Nanocomposites with Carbon Nanotubes: Structural Features and Properties
Authors: Cerrada María L. , Arranz-Andrés Javier , Caballero-González Alicia , Blázquez-Blázquez Enrique , Pérez Ernesto
Published: March 2023

Different amounts of carbon nanotubes (CNT) have been incorporated in materials based on poly(vinylidene fluoride) (PVDF) by solvent blending followed by their further precipitation. Final processing was performed by compression molding. The morphological aspects and crystalline characteristics have been examined, additionally exploring in these nanocomposites the common routes described in the pristine PVDF to induce the β polymorph. This polar β phase has been found to be promoted by the simple inclusion of CNT. Therefore, coexistence of the α and β lattices occurs for the analyzed materials. The real-time variable-temperature X-ray diffraction measurements with synchrotron radiation at a wide angle have undoubtedly allowed us to observe the presence of the two polymorphs and determine the melting temperature of both crystalline modifications. Furthermore, the CNT plays a nucleating role in the PVDF crystallization, and also acts as reinforcement, increasing the stiffness of the nanocomposites. Moreover, the mobility within the amorphous and crystalline PVDF regions is found to change with the CNT content. Finally, the presence of CNT leads to a very remarkable increase in the conductivity parameter, in such a way that the transition from insulator to electrical conductor is reached in these nanocomposites at a percolation threshold ranging from 1 to 2 wt.%, leading to the excellent value of conductivity of 0.05 S/cm in the material with the highest content in CNT (8 wt.%).
In Situ Nanofibrillar Polypropylene-Based Composite Microcellular Foams with Enhanced Mechanical and Flame-Retardant Performances
Authors: Jiang Yufan , Jiang Jing , Yang Lian , Zhang Yihe , Wang Xiaofeng , Zhao Na , Hou Jianhua , Li Qian
Published: March 2023

With the increasing demand for plastic components, the development of lightweight, high strength and functionalized polypropylene (PP) from a cost-effective and environmentally friendly process is critical for resource conservation. In situ fibrillation (INF) and supercritical CO2 (scCO2) foaming technology were combined in this work to fabricate PP foams. Polyethylene terephthalate (PET) and poly(diaryloxyphosphazene)(PDPP) particles were applied to fabricate in situ fibrillated PP/PET/PDPP composite foams with enhanced mechanical properties and favorable flame-retardant performance. The existence of PET nanofibrils with a diameter of 270 nm were uniformly dispersed in PP matrix and served multiple roles by tuning melt viscoelasticity for improving microcellular foaming behavior, enhancing crystallization of PP matrix and contributing to improving the uniformity of PDPP’s dispersion in INF composite. Compared to pure PP foam, PP/PET(F)/PDPP foam exhibited refined cellular structures, thus the cell size of PP/PET(F)/PDPP foam was decreased from 69 to 23 μm, and the cell density increased from 5.4 × 106 to 1.8 × 108 cells/cm3. Furthermore, PP/PET(F)/PDPP foam showed remarkable mechanical properties, including a 975% increase in compressive stress, which was attributed to the physical entangled PET nanofibrils and refined cellular structure. Moreover, the presence of PET nanofibrils also improved the intrinsic flame-retardant nature of PDPP. The synergistical effect of the PET nanofibrillar network and low loading of PDPP additives inhibited the combustion process. These gathered advantages of PP/PET(F)/PDPP foam make it promising for lightweight, strong, and fire-retardant polymeric foams.
Effects of Spray Drying, Freeze Drying and Gamma Irradiation on the Antioxidant Activities of Camel and Cow Milk Fractions
Authors: Harizi Nouha , Madureira Joana , Zouari Ahmed , Ayadi Mohamed Ali , Cabo Verde Sandra , Boudhrioua Nourhène
Published: March 2023

This work aimed to establish an integrated approach to investigate the total phenolic content and antioxidant activities of dried skim camel and cow milk and their fractions. The milk fractions were obtained by acid or enzymatic coagulation followed by spray drying (inlet temperature/outlet temperature: 125 ± 2 °C/90 ± 2 °C) or freeze drying (−50 °C, 0.05 mbar) coupled or not to gamma irradiation (at 5, 11, 22 kGy). The results showed that the total phenolic content (measured in gallic acid equivalent, GAE) varied depending on the drying technique. The freeze-drying process corresponded to the highest values of total phenolic compounds, with 247.23 ± 2.08 mg GAE/100 g powder for the β-casein fraction of camel milk (βC CaM) and 621.13 ± 4.16 mg GAE/100 g powder for the β-casein fraction of cow milk (βC CoM). Compared to spray-dried fractions, freeze-dried fractions showed generally higher ferric reducing antioxidant power for both camel milk and cow milk. The highest values of free radical scavenging activity were seen in the spray-dried β-casein fractions of camel milk (βC CaM) and cow milk (βC CoM) and in the freeze-dried acid whey of cow and camel milk (AW CaM and AW CaM). Freeze-dried acid whey (AW CaM and AW CoM) appeared to be less sensitive to gamma irradiation at 5 and 11 kGy.
Enhanced Enzymatic Hydrolysis of Wheat Straw to Improve Reducing Sugar Yield by Novel Method under Mild Conditions
Authors: Zhao Xuyang , Han Lihua , Ma Xuejiao , Sun Xiaoran , Zhao Zheng
Published: March 2023

Wheat straw is a suitable source material for bioethanol production. Removing lignin and hemicellulose in wheat straw to improve enzymatic hydrolysis efficiency is essential because of its complex structure. Deep eutectic solvents (DESs) have become substitutes for ionic liquids (ILs), with the characteristics of good biocompatibility, simple synthesis procedure and low cost. However, the process of removing lignin and hemicellulose using present DESs requires a high operation temperature or long operation time. Therefore, we studied a novel method under mild conditions for screening a series of novel DESs based on an inorganic base to remove lignin and hemicellulose in wheat straw. In this work, the effect of DES type, the pH of the DESs, the operation temperature and operation time for enhancing enzymatic hydrolysis, and the crystal structure and the chemical structure and surface morphology of wheat straw were investigated. In particular, Na:EG exhibited the most excellent solubility for wheat straw under mild conditions, removing 80.6% lignin and 78.5% hemicellulose, while reserving 87.4% cellulose at 90 °C for 5 h, resulting in 81.6% reducing sugar produced during hydrolysis for 72 h. Furthermore, XRD, FT-IR and SEM analysis verified the lignin and hemicellulose removal. Hence, DESs based on an inorganic base used for removing lignin and hemicellulose will enhance enzymatic hydrolysis, and thus promote the industrial application of wheat straw to produce bioethanol.
Variable Universe Fuzzy Controller for an Independent Metering System of Construction Machinery
Authors: Hu Shuang , Wang Lihang , Li Yongquan , Zhang Lijie
Published: March 2023

As a new type of hydraulic system, the independent valve control system (IMCS) has higher flexibility compared with the traditional four-side slide valve control system. The independent metering control system (IMCS) is divided into four quadrant modes, based on the speed and load of the actuator. The traditional control methods always use a discrete control strategy to realize the switching between different modes based on mode recognition. However, the spool signals frequently switch, and the actuator operating pressure and speed are not stable when the IMCS is switched between different modes. To solve the above problems, this paper proposes a continuous control strategy for the IMCS. Based on the fuzzy control principle, the variable universe fuzzy controller (VUFC) was designed. The controller contains 49 fuzzy rules, and the output of the controller at any time is calculated by the weighted sum of several fuzzy rules. The VUFC divides the system into 49 rules, which are activated by multiple modes at any time to achieve a smooth transition between different modes. The VUFC was verified in a 37 ton excavator model and compared with the traditional independent metering meter-out (IM_MO) control strategy. The results showed that the VUFC significantly improved the frequent switching of the spool signals, and the running speed and pressure stability of the hydraulic cylinder were significantly improved. At the same time, the response speed of the hydraulic cylinder became faster at the start of the system. The VUFC can realize the continuous control of the IMCS and improve the operating performance of excavators.
Research on Multiple Constraints Intelligent Production Line Scheduling Problem Based on Beetle Antennae Search (BAS) Algorithm
Authors: Zhang Yani , Xu Haoshu , Huang Jun , Xiao Yongmao
Published: March 2023

Aiming at the intelligent production line scheduling problem, a production line scheduling method considering multiple constraints was proposed. Considering the constraints of production task priority, time limit, and urgent task insertion, a production process optimization scheduling calculation model was established with the minimum waiting time and minimum completion time as objectives. The BAS was used to solve the problem, and a fast response mechanism for emergency processing under multiple constraints was established. Compared with adaptive particle swarm optimization (APSO) and non-dominated sorting genetic algorithm-II (NSGA-II) operation, this algorithm showed its superiority. The practical application in garment processing enterprises showed that the method was effective and can reduce the completion time and waiting time.
Fluctuation in the Water Level of the Air Hole of the Gate Shaft in the Pumped Storage Power Station
Authors: Zhao Weiguo , Li Shuo , Fan Honggang , Wang Liying
Published: March 2023

In some pumped storage stations, water spray from the air hole occurs during load rejection. In order to avoid this phenomenon, it is necessary to study the change of the air hole water level during the transient process of the power station. A transient simulation of a pumped storage power station was carried out to study the variation in the maximum water level of the air hole and gate shaft. The transition process of a power station with or without a surge tank was analyzed, and the changes in the water level of the gate shaft and air hole were compared based on different gate shaft areas, and the influence of the gate shaft location on the changes in the water level of the gate shaft and air hole was determined. The following results were obtained: when the power station has a surge tank, the maximum water levels of the gate shaft and air hole are basically consistent; when the station has no surge tank, the maximum water level difference between the gate shaft and air hole gradually increases as the gate shaft area increases; the greater the distance between the gate shaft and inlet, the larger the maximum water level difference between the air hole and gate shaft; overflow measures for the air hole are required if the maximum water level of the air hole exceeds the design value.