bluuebunny/crossref_metadata_2025 huggingface dataset:
Allergens in sesame oil contact dermatitis
Authors: Neering H , Vitányi BE , Malten KE , Van Ketel WG , Van Dijk
Published: July 2023

In 13 patients with contact allergy to sesame oil, studies were undertaken to elucidate the nature of the allergens. Sesamol, sesamin and sesamolin were identified in crude and purified (pharmaceutical) sesame oil. Patch tests showed 8 of the 13 patients to be positive to sesamol and 12 to sesamolin and sesamin. Patch tests with the pure substances on thin-layer sheets were inconclusive as to any difference between these substances. Group allergy to several substances related to sesamol could not be clearly demonstrated.
Scanning electron microscopy of acarus scabiei
Authors: Juhlin L , Brunk U , Fredriksson BA.
Published: July 2023

Details in the anatomy of sarcoptes scabiei var. hominis are described. Scanning electron microscopy is of help in illustrating this parasite which is commonly seen in clinical practice. The technique also provides the possibility to differentiate between various types of mite.
Treatment of familial benign chronic pemphigus by skin grafting
Authors: Sonck CE , Rintala A.
Published: July 2023

A report on a patient with familial benign chronic pemphigus, resistant to all conservative therapy, treated in several stages by surgical excision of the most affected areas and reconstruction with split skin grafts. The follow-up period of the treated sites varies from two to eight years. All grafted sites have remained totally asymptomatic and there has been no sign of recurrence. Outside the grafted areas the disease is still as active as before.
Letter: Report of the meeting of the international committee of dermatology in Mexico City, October 31 to November 2, 1974
Authors: Listed No authors
Published: July 2023

Abstract is not available.
A clinical pattern for making an immediate-presumptive diagnosis of gonococcal infection in women
Authors: Wallin J.
Published: July 2023

Abstract is not availeble.
Ultrastructure of systemic lupus erythematosus. Dermal connective tissue
Authors: Kobayasi T , Asboe-Hansen G.
Published: July 2023

Abstract is not availeble.
Heparin-precipitable fraction (HPF) in an unselected material of dermatological inpatients
Authors: Fyrand O.
Published: July 2023

Abstract is not availeble.
Zinc therapy in acrodermatitis enteropathica
Authors: Michaëlsson G.
Published: July 2023

Abstract is not availeble.
Acrodermatitis enteropathica. Report of a case healed with zinc therapy
Authors: Thyresson N.
Published: July 2023

Abstract is not availeble.
Plasma kinins and the jarisch-herxheimer reaction
Authors: Zachariae H , Nielsen E.
Published: July 2023

Abstract is not availeble.
Precipitating antibodies to sulfathiazole in serum of patients with suspected allergy to sulfonamides
Authors: Christiansen AH.
Published: July 2023

Abstract is not availeble.
Trace elements with suspected and hitherto unknown biological function in normal and psoriatic epidermis
Authors: Molin L , Wester PO.
Published: July 2023

Abstract is not availeble.
Contamination of leg ulcers. A bacteriological and clinical study on the effect of treatment with an ointment containing ristocein A and circulin
Authors: Kamp P , Perdrup A.
Published: July 2023

Abstract is not availeble.
Erythema dyschromicum perstans. A report of two cases in fair-skinned patients
Authors: Byrne DA , Berger RS.
Published: July 2023

Abstract is not availeble.
Deposition of fibrinogen (FR-antigen) in skin diseases. II. Pustulosis palmaris et plantaris (with special reference to heparin-precipitable fraction)
Authors: Fyrand O , Mellbye OJ , Larsen TE.
Published: July 2023

Ten out-patients with pustulosis palmaris et plantaris were examined with direct immunofluorescence (IF) technique for deposition of fibrinogen, fibrin or its degradation products (FR-antigen) in affected and unaffected skin, together with heparin-precipitable fraction (HPF), cryoglobulin and total plasma fibrinogen in the blood. FR-antigen was found in all cases in affected skin as a uniform pattern of a continuous ramification below the dermo-epidermal junction. This IF picture was absent in unaffected skin, but in other parts of the dermis in affected and unaffected skin, scattered streaks of IF could be seen. In one case, however, unevenly distributed IF was found in unaffected skin in the junction area. The scattered IF was also present in a minor degree in affected and unaffected skin in a control material of other dermatoses. Only one patient had slightly elevated values of HPF (0.33 mg/ml). Total plasma fibrinogen was insignificantly elevated, and no cryoglobulin could be found.
Penetration kinetics of four drugs in the human skin. Influences of various pharmaceutical vehicles on penetration in vitro
Authors: Zesch A , Schaefer H.
Published: July 2023

Abstract is not availeble.
Asymptomatic male and female gonorrhoea
Authors: Nielsen R , Søndergaard J , Ullman S.
Published: July 2023

Three hundred and ninety-nine patients attending the out-patient clinic of the Department of Dermatology and Venereology, Rigshospital, Copenhagen, for various venereal complaints were examined during a three-month trial. Neisseria gonorrhoeae was found in 75 female and 89 male patients. 43 women (57%) and 13 men (15%) were asymptomatic at the first visit. The duration of the asymptomatic period exceeded 3 weeks in 25 female (33%) and 7 male (8%) patients. The asymptomatic period in the male group varied from 15 to 90 days and from 4 to 180 days in the females. The majority of the patients with asymptomatic gonorrhoea had been exposed to a sexual contact with proven gonococcal infection, thus emphasizing the importance of contact investigation.
Cell-mediated immunity in chronic polymorphous light eruptions. Leukocyte migration inhibition assay with irradiated skin as antigen
Authors: Jansén CT , Helander I.
Published: July 2023

Homogenates of irradiated and non-irradiated skin from 16 patients with chronic polymorphous light eruptions (CPLE) were examined to detect possible light-induced antigen to cell-mediated immunity. The leukocyte migration agarose tests which were performed disclosed no evidence of migration inhibition in either type of skin. These findings do not support the theory of CPLE being a delayed hypersensitivity reaction against an antigen formed in the skin by actinic radiation.
Immunological phenomena induced by UV rays
Authors: Jarzabek-Chorzelska M , Zarebska Z , Wolska H , Rzesa G.
Published: July 2023

Antiserum against UV-irradiated DNA was prepared and used as a specific reagent in an indirect immunofluorescence (IF) technique for the detection of photochemically damaged DNA in the epidermis of hairless mice. Fluorescence of cell nuclei was found in sections of dorsal epidermis of mice immediately after the irradiation. It persisted for 24 hours. The IF reaction became negative after 48 hours, irrespective of the duration of UV exposure to which the mice were subjected. This may indicate: either (a) the occurrence of DNA repair processes, or (b) the relation of DNA repair to the life cycle of the epidermal cell. Mice exposed to UV radiation similar to the erythema spectrum of sunlight do not show any changes in the cellular DNA, even after a dose of 40 MED.
Demonstration of 3-methoxytyrosine in the urine of melanoma patients
Authors: Agrup G , Bengtsson M , Falck B , Persson K , Rorsman H , Rosengren AM , Rosengren E.
Published: July 2023

With the aid of gas chromatography and mass spectrometry a metabolite of dopa, 3-methoxytyrosine, has been demonstrated in the urine of 2 patients with melanoma metastases, the amounts excreted being 4.0 mg and 8.5 mg/24 hours, respectively. 3-Methoxytyrosine could not with certainty be identified in the urines of two normal subjects.
Plasma concentrations of griseofulvin in healthy volunteers and out-patients treated for onychomycosis
Authors: Hägermark O , Berlin A , Wallin I , Boréus LO.
Published: July 2023

Therapeutic failure of griseofulvin therapy are fairly common, espcially when toe nail infections are treated. The reasons for the failures remain largely unknown. The aim of the present investigation was to study whether pharmacokinetic factors may be responsible. At first, single-dose and steady-state pharmacokinetics of griseofulvin were analysed in volunteers by means of a gas-chromatographic technique. It was found that there was no difference in plasma levels of the three brands of griseofulvin commercially available in Sweden, whereas there was a singificant difference of absorption between individuals. The plasma half-life varied considerably from day to day in the same individual. In the second part of the investigation griseofulvin plasma concentrations were determined in 27 patients treated with griseofulvin for onychomycosis and related to the therapeutic result. All patients but one were initially improved but the therapeutic effect faded in 15 patients after 5-20 months of treatment ("partially healed" patients). In 11 patients the nail infections were clinically healed after 6-24 months. However, no statistically significant difference of the plasma griseofilvin levels could be demonstrated between the healed and partially healed groups. Some possible reasons for the therapeutic failure are discussed.
Miconazole in the treatment of candidiasis of the digestive tract
Authors: Svejgaard E.
Published: July 2023

Miconazole nitrate tablets were used in the treatment of candidiasis of the oral cavity and the digestive tract. In 5 out of 6 patients with severe, predisposing factors and who had received previous unsuccessful antimycotic therapy, clinical improvement occurred after a few days of miconazole treatment. Mycological cure of these 5 patients was achieved after 1-11 weeks of treatment. Three of the 5 cured patients relapsed after 3 months, due to basic failure. One patient complained of the drug's unpleasant taste. Otherwise, no side effects were observed. There was no apparent development of any resistance to the drug.
The nitroblue tetrazolium test in acne vulgaris
Authors: Roberts MM , Tan SG , Cunliffe WJ , Cotterill JA.
Published: July 2023

The nitroblue tetrazolium (N.B.T.) test has been studied in 19 patients with moderate or severe acne vulgaris and 18 patients with subclinical or no acne. There was a significant elevation of the N.B.T. score in those patients with moderate or severe acne as compared with the control group. This is probably a result of the inflammatory reaction in and around the involved sebaceous gland follicles.
Study of some minerals in normal Egyptian scalp hair
Authors: Aal HA , Soliman AA , Elmahdy H , El Saeei L.
Published: July 2023

Scalp hair of 128 normal individuals of both sexes and of differing ages was examined for its mineral content by flame photometric and colorimetric procedures. The results obtained revealed that sodium and potassium may be considered as sex specific and of value as a medico-legal sign, while no marked difference was observed between male and female calcium content. The values of scalp hair copper were found to be slightly higher in females than in males. Similarly the mean value of scalp hair iron was found higher in females than in males, especially during the female reproductive period. Regarding the scalp hair phosphorus content, it has been found that a relationship between phosphorus and calcium does exist.
Exaggerated onychodermal band associated with unilateral racket thumb nail
Authors: Daughters DB , Maibach HI.
Published: July 2023

Exaggerated onychodermal bands and a unilateral racket thumb nail occurred in a healthy 36-year-old primiparous female. Each condition is described and the relationship of exaggerated onychodermal bands to chronic renal disease (systemic disease) is reviewed. We are reporting this patient since we have been unable to find a similar reported case with both abnormalities and because of the paucity of information on these conditions in the dermatology literature.
Neurotoxicity--a side-effect of sulphones
Authors: Volden G.
Published: July 2023

Abstract is not available.
5-S-cysteinyldopa in the urine of melanoma patients
Authors: Agrup G , Agrup P , Andersson T , Falck B , Hansson JA , Jacobsson S , Rorsman H , Rosengren E , Rosengren AM.
Published: July 2023

A newly discovered amino acid, 5-S-cysteinyldopa, present in the urine of healthy subjects is excreted in pathological amounts in many patients suffering from melanoma metastases. Increased excretion of 5-S-cysteinyldopa may be observed before metastases become clinically evident. Determination of 5-S-cysteinyldopa is superior to determination of dopa+dopamine in the diagnosis of melanoma metastases.
Investigations on the binding sites of the basement membrane zone for pemphigoid antibodies in vitro. II. Immunohistochemical reactions of the reactive groups
Authors: Bockendahl H , Remy W , Remy B , Petersen G , Stüttgen G.
Published: July 2023

In continuing our studies on the characteristics of the antigenic structures of the basement membrane, procedures used in histochemistry for the identification and blocking of reactive groups have been adapted in this present work to the pretreatment of cryostat sections of guinea-pig tongue. These sections have then been studied by the indirect immunofluorescence method. By means or reagents, which produce a reversible extinction of the basement membrane fluorescence produced with pemphigoid sera, it proved possible to determine the existence of certain chemical groupings which bind the anti-basement membrane antibodies, namely: free carboxyl groups deriving from amino acids, hydrogen bridges in which the hydroxyl groups from the carbohydrates in the antigen are probably involved, bonds involving the free aldehydes of the antigen (either cross-links from aldehydes, or formation of Schiff's bases with amino groups of the antibodies), and lastly, to a lesser extent, disulphide bonds. The antigen/antibody reaction probably occurs in the neighbourhood of the alpha-glucosido-beta-galactosido-hydroxylysine. A reaction model has been developed from these findings. There were also some indications that the antigenic structures of the intercellular substance are chemically related to those of the basement membrane. The cell nucleus antigens, on the other hand, appear to possess different chemical properties.
Studies on carrier protein in contact dermatitis: macrophage migration inhibition by soluble epidermal substances as carrier proteins
Authors: Miyagawa N , Miyagawa S , Ashizawa T , Sakamoto K , Aodi T.
Published: July 2023

The migration of peritoneal exudate cells from picrylchloride-sensitive guinea pigs can be inhibited by the water-soluble proteins present in the picrylchloride-treated guinea pig epidermis. Further examination of these proteins revealed that antigens were present in fractions with the molecular weights ranging between 10 000 and 200 000.
Patch testing with mineral wool (Rockwool)
Authors: Björnberg A , Löwhagen GB.
Published: July 2023

25% of 315 tested subjects exhibited skin reactions when patch tested with mineral wool. Coating of the mineral fibres with phenol-formaldehyde did not influence the skin reactions. The reactions seem to be induced mechanically, as the mineral without the fibres did not give any reactions. No allergic reactions to the chemical additives were demonstrated. Macroscopically, the reactions may simulate an allergic response; microscopically, they seem to be toxic, sometimes with prominent spongiosis.
Intracellular distribution of dopa and 5-S-cysteinyldopa in Harding-Passey melanoma
Authors: Agrup G , Falck B , Rorsman H , Rosengren AM , Rosengren E.
Published: July 2023

The concentrations of dopa and 5-S-cysteinyldopa were determined in the various cell fractions of Harding-Passey melanomas. Dopa was present in larger amounts than was 5-S-cysteinyldopa in all cell fractions, but the dopa/5-S-cysteinyldopa ratio was lower in the soluble fraction and in the small-granule fraction than in the large-granule fraction. The soluble fraction contained the greatest amount of catechols. These findings are compatible with high tyrosinase activity not only in the melanosomes but also in the small-granule and soluble fractions.
Comparative treatment of psoriasis with UV-light, trioxsalen plus UV-light, and coal tar plus UV-light
Authors: Fischer T.
Published: July 2023

Areas within psoriasis plaques have been treated with UV-light alone, trioxsalen plus UV-light, and coal tar (liquid carbonis detergens) plus UV-light, using the 313, 365 and 405 nm wavelength bands in various doses. With the 313 nm band, the same degree of healing was obtained with all three types of treatment. With the 365 nm band, no healing was achieved with UV-light alone, but healing did occur with UV-light when the skin was sensitized with trioxsalen. Coal tar itself has a certain healing effect on psoriasis but the effect is so markedly enhanced by light of the 365 nm band that the healing is comparable to that obtained with the 313 nm band. No healing was seen with light of the 405 nm band. The patients who were healed with the 313 nm band alone, also healed with trioxsalen or coal tar plus UV-light of the 365 nm band. With each method there is an individual optimal light dose of 1--2 MED that gives healing.
Human skin proteases: effect of separated proteases on vascular permeability and leukocyte emigration in skin
Authors: Fräki JE.
Published: July 2023

The effect of human skin proteases on vascular permeability and leukocyte emigration in rabbit skin was investigated. The alkaline protease of human skin capable of hydrolysing trypsin substrate effectively increased vascular permeability. This effect was not inhibited by antihistamine, but almost totally so by Trasylol. The reaction was protracted. Leukocyte emigration in skin, primarily of PMN-cells at 12 hrs, and later a migration of mononuclear cells, also resulted. Swelling of the dermal fibres was noted. The alkaline protease of human skin capable of hydrolysing chymotrypsin substrate also increased vascular permeability, but this phenomenon was effectively inhibited by antihistamine and the reaction was of brief duration. The leukocyte emigration caused by this enzyme was remarkable. The acid proteases of human skin resembling cathepsin B1 and D also caused brief increased vascular permeability, which was effectively inhibited by antihistamine. The cellular reactions to these acid proteases were mild. The role of protease inhibitors in skin in the enzyme reactions is discussed.
Azapropazone induced bullous drug eruptions
Authors: Barker DJ , Cotterill JA.
Published: July 2023

Azapropazone is a new, powerful, antiinflammatory analgesic. Initial trails have confirmed its efficacy as an antirheumatic agent, with a low incidence of adverse reactions. Recently the authors have seen two patients, each of whom developed a distinctive but clinically dissimilar, bizarre, bullous eruption after taking this drug. A brief account of these cases has been published elsewhere (1).
Immunoelectron microscopy of linear dermatitis herpetiformis: report of a case
Authors: Pehamberger H , Konrad K , Stingl G , Holubar K.
Published: July 2023

Linear deposits of both IgA and C3 were found along the BMZ (by direct IF), and band-like IgA deposits directly below the basal lamina (by HRP-anti-HRP electron microscopy), in a case of clinically typical DH. No circulating antibodies could be detected.
Ultrastructure of dermal connective tissue in fibrodysplasia ossificians progressiva
Authors: Hentzer B , Kobayasi T , Asboe-Hansen G.
Published: July 2023

The ultrastructure of dermal connective tissue in two patients suffering from fibrodysplasia ossificans progressiva (syn myositis o.p.) was studied. The disorder starts with soft tissue swelling which subsequently ossify. The connective tissue of various organs may be affected. No defects in calcium metabolism have been discovered. It was intended to study the primary event which initiates the ossification. The present ultrastructural study demonstrates an accumulation of proteoglycan microfibrils as well as glycoprotein material in dermal connective tissue. Both substances are prerequisites of calcification resulting from the binding of calcium and phosphorus ions. A subsequent release of proteolytic enzymes in the tissues may be set free the bound iones and, thus, initiate mineralization.
Chronic lymphatic leukaemia, malignant melanomas and mosquito hypersensitivity
Authors: Lidén S , Bäck O , Tärnvik A.
Published: July 2023

Abstract is not available
Nevus comedonicus. Report of three cases localized on glans penis
Authors: Abdel-Aal H , Abdel-Aziz AM.
Published: July 2023

Abstract is not available.
Excretion of 5-S-cysteinyldopa in the urine of healthy subjects
Authors: Agrup G , Falck B , Fyge K , Rorsman H , Rosengren AM , Rosengren E.
Published: July 2023

Seventy-six Caucasians, 30 men and 46 women, were investigated for the 24-hour excretion of 5-S-cysteinyl dopa in the urine during the months of September to November, 1973. No subject had had strong sun exposure for at least 4 weeks prior. A preliminary finding of a variation of 5-S-cysteinyldopa with season necessitated this precaution. The excretion varied between 9.0 and 242 mug/24 hours. The mean value in men was 100 mug/24 hours and in women 77.8 mug/24 hours. Subjects with white hair had lower values than those with pigmented hair, but there was no other difference between the excreted amounts in subjects with differing hair colour. There was no variation with age when the subjects with white hair were excluded. No variation with weight or body surface was found. Excretion of dopa and dopamine determined together did not correlate with the excretion of 5-S-cysteinyldopa.
Hydroxyproline to hydroxylysine molar ratio indicates collagen type
Authors: Blumenkrantz N , Asboe-Hansen G
Published: July 2023

By using the molar ratio Hyp to Hyl, types I and II of collagen can be differentiated in 0.5 to 10 mg of dried, defatted tissue. Analyses on human skin, tendon, bone, aorta, cartilage, as well as nucleus pulposus, and annulus fibrosus of intervertebral discs are reported. Analyses of collagen of mesenchymal tissues of other vertebrates are also reported. From amino acid analysis of purified collagen samples published in the literature, the molar ratio Hyp/Hyl was calculated. Type I and type III collagen were differentiated from type II, and the latter was differentiated from type IV collagen. The molar ratios obtained with our analyses followed closely the values from previously reported amino acid analyses on purified collagen.
Immunofluorescence of the skin in allergic diseases: an investigation of patients with contact dermatitis, allergic vasculitis and atopic dermatitis
Authors: Secher L , Permin H , Juhl F
Published: July 2023

Skin biopsies for immunofluorescent studies were taken from patients with contact dermatitis (positive patch tests), atopic dermatitis and allergic vasculitis for comparison with normal-appearing skin from the same patients, and from healthy controls. A variety of deposits of immunoglobulins, complement components and fibrinogen were demonstrated in 6 out of 20 patients with contact dermatitis, 7 out of 10 with atopic dermatitis, 8 out of 10 with allergic vasculitis, and in 4 out of 20 control individuals. No diagnostic pattern of deposits was found. Elevated serum IgE and eosinophilic counts were found in patients with atopic dermatitis, and high serum IgA and fibrinogen levels were found in the allergic vasculitis group.
Studies on the cutaneous sensitization reaction of guinea pigs to purified aromatic chemicals
Authors: Senma M , Fujiwara N , Sasaki S , Toyama M , Sakaguchi K , Takaoka I
Published: July 2023

The sensitization reactions to purified cinnamic aldehyde and its chemically related substances were investigated using the maximization test on guinea pigs. Mutual cross-sensitivities were also examined. Cinnamic aldehyde was found to be the most potent sensitizer in this series, and a strong cross-sensitizer. From the chemical structures of these substances, the following speculations were made. There was a tendency that as the number of hydrocarbons of alkyl groups replacing the alpha-hydrogen in the molecule increased, the rate of sensitization reaction declined. The conjugated system of double bonds was suspected to be a determinant factor in relation to the sensitization ability of the substances in this series.
Successful PUVA-treatment in the tumour stage of mycosis fungoides associated with the appearance of lesions in organs other than the skin
Authors: Molin L , Skogh M , Volden G
Published: July 2023

Abstract is not available
Pemphigus acantholysis in tissue culture: studies on photo-induction
Authors: Gschnait F , Pehamberger H , Holubar K
Published: July 2023

It has previously been shown tha UV irradiation can give rise to fresh skin lesions in the uninvolved skin of patients with pemphigus. In this study no increase of acantholytic activity was found in an organ culture model system for pemphigus in vitro, after in vitro and in vivo irradiation under various experimental conditions. By immunofluorescence no increase in IgG binding in irradiated specimens was observed. From these in vitro findings, which contrast the effect of UV light in pemphigus in vivo, it may be concluded that the circulatory system of the skin plays a major role in the photo induction of acantholysis in pemphigus.
Pemphigus vulgaris in a 15-year-old girl
Authors: Harrington I , Sneddon IB , Walker AE
Published: July 2023

Pemphigus vulgaris is uncommon in adolescence and only ten well documented cases in this age group were found in a recent review (5). Because the condition is often more severe in the younger age group it is important to consider it in the differential diagnosis of bullous eruptions of childhood and to perform direct and indirect immunofluorescence studies.
Palpebral cellulitis
Authors: Roed-Petersen J , Larsen HW
Published: July 2023

Patients with palpebral cellulitis may turn in the first place to a skin clinic. The clinical picture and the course of disease are illustrated here by four case histories. The importance of differentiating between collateral orbital edema, palpebral cellulitis and orbital cellulitis is stressed.
Chronic bullous dermatosis of childhood Immunological and ultrastructural studies on the melanocyte--dermal junction
Authors: Ortonne JP , Schmitt D , Jacquelin L
Published: July 2023

Ultrastructural study of a case of benign chronic bullous dermatosis of childhood revealed the presence of melanocytes in the base of the blister. The melanocyte anchorage mechanism is described. The absence of labelling by bullous pemphigoid antibodies on the base of the blister, where melanocytes persist, indicates that the antigens recognized by these autoantibodies do not exist in the melanocyte basal membrane-contact zones. This suggests that the chemical nature of the melanocyte dermal junction anchorage system is different from that of the hemidesmosomes.
Trichochromes in human malignant melanoma
Authors: Agrup G , Hansson C , Rorsman H , Rosengren AM , Rosengren E
Published: July 2023

Decarboxytrichochromes B and C were isolated from a melanoma metastasis in a red-haired man, indicating the presence of trichochromes B and C in the tissue. Trichochromes E and F were not detected.
Levamisole-induced hypersensitivity
Authors: Secher L , Permin H , Skov PS , Ullman S , Halberg P
Published: July 2023

A 73-year-old woman with rheumatoid arthritis was treated with Levamisole, 150 mg per day, on 2 days a week. Her arthritis improved, but she developed a severely itching rash, and the treatment was stopped after 6 months. Penicillamine was subsequently given and tolerated without skin complications. 15 months after regular Levamisole was stopped, she was given a single dose of 150 mg which provoked fever of 40 degrees C and rash. Thirteen punch-biopsy specimens were examined by direct immunofluorescence microscopy. During the Levamisole treatment, granular deposits of IgG and C3 were found at the dermal--epidermal junction. Subsequently, the deposits disappeared, but reappeared after Levamisole challenge. The patient's leukocytes were exposed in vitro to Levamisole, and 36% of the total histamine content in the basophils was released. Our results provide further evidence that Levamisole can cause type-I as well as type-III hypersensitivity.
Submicroscopic aspects of the keratinization, dyskeratinization and acantholysis of fogo selvagem
Authors: Lagerholm B , Frithz A
Published: July 2023

Histopathological, cytomorphological and electron microscopic analyses in a case of fogo selvagem are reported. Contradictory to the light microscopical findings, the acantholysis as seen with the electron microscope involves the basal layer but not the subcorneal layers--at least not the most superficial part of the granular layer. A conspicuous disintegration of the tonofilament-desmosome complexes give rise to the concomitant dyskeratosis. This aberrant process, including an association between retracing tonofilaments and defective Odland bodies, results in the terminal stage of monstrous defective and specific keratohyalin. In cytoplasm, target-like structures similar to virions of the herpes virus group were observed. The dynamics of the pathological process is discussed.
DNA interstrand crosslinks in normal human skin visualized by electron microscopy
Authors: Bohr V , Wadskov S , Søndergaard J , Lerche A
Published: July 2023

Normal human skin was investigated for DNA interstrand crosslinks by a denaturation electron microscopic method. Skin biopsy materials from the thighs of 8 healthy volunteers were separated into epidermis and dermis. DNA from each layer was isolated, purified and studied in the electron microscope. The presence of DNA interstrand crosslinks in normal human skin was demonstrated. The number of crosslinked DNA molecules and the density of crosslinking within the molecules were determined. Crosslinks were found in 0.8% of the scored molecules and were located almost exclusively in epidermal DNA. The intramolecular density of crosslinking showed a wide range of variation in each volunteer and between the volunteers.
Ultrastructural changes in nuclei and nucleoli in psoriatic epidermis during and after PUVA therapy
Authors: Tsuji T , Cox AJ
Published: July 2023

Quantitative and qualitative changes of the nuclei and nucleoli in psoriatic epidermis at different intervals during therapy with psoralen and long-wave ultraviolet light (PUVA) were revealed with the electron microscope. Early in the therapy (24 to 96 hr after commencing treatment) changes occur, including irregularity of the nuclear envelope, enlargement of the nuclei and nucleoli, temporary disappearance of fibrillar centres, followed by temporary disappearance of fibrillar centres, followed by temporary increase in nuclear bodies and increasing amounts of heterochromatin. After clearing of lesions, the nuclear and nucleolar structures were identical with those of normal keratinocytes, except that the relative amount of heterochromatin was greater. It is suggested that PUVA therapy brings about inhibition of abnormal keratinization, possibly due to gene repression, and that this may be related to changes in the fibrillar centres of psoriatic keratinocyte nucleoli.
A leukotactic factor in the stratum corneum of pustulosis palmaris et plantaris: a possible mechanism for the formation of intra-epidermal sterile pustules
Authors: Tagami H , Ofuji S
Published: July 2023

Previous studies on the chemotactic properties of soluble substances from psoriatic scales demonstrated the presence of complement-derived chemotactic factors (anaphylatoxins) that seem to play a crucial role in the production of typical psoriasiform tissue changes. The crude extract from the stratum corneum of lesions of pustulosis palmaris et plantaris (PPP) was also noted to contain highly chemotactic components for polymorphonuclear leukocytes (PMN's) as compared with a crude callus extract in the present study. The leukotactic factors contained in the major chemotactic fractions isolated by Sephadex G-75 chromatography revealed in vitro characteristics and in vivo biological activities very similar to those of the leukotactic factor derived from psoriasis scale reported in earlier work, i.e. complement cleavage products (C3a and C5a). On the basis of these findings we have reason to believe that pustule formation in PPP occurs through a mechanism that has a strong resemblance to that in pustular psoriasis. We hypothesize further that similar mechanism may be operative in other dermatoses in which the formation of sterile subcorneal pustules is a characteristic feature.
Phototoxic reaction to long-term low-dose administration of chlorpromazine in mice
Authors: Ljunggren B , Möller H
Published: July 2023

The mouse tail in vivo technique for the study of acute drug phototoxicity was tested in experiments involving prolonged administration of chlorpromazine and ultraviolet light. A phototoxic reaction was induced with lower combined doses of drug and radiation than was the case in single exposures.
Phylogenetic studies with pemphigus and pemphigoid antibodies
Authors: Diaz LA , Weiss HJ , Calvanico NJ
Published: July 2023

Indirect immunofluorescence (IF) studies were performed on skin from a variety of vertebrate specimens and IgG fractions from pemphigoid and pemphigus sera. Pemphigoid antigen was present in fish, amphibian, reptilian, avian and mammalian skin, whereas pemphigus antigen was observed in avian and mammalian skin only.
A pustular eruption of the chin (a variant of pyoderma faciale?)
Authors: Barker DJ , Gould DJ
Published: July 2023

Two cases are reported of patients who developed a dramatic pustulo-nodular rash on the chin. The onset was explosively sudden and cosmetically disabling. Ultimately, resolution occurred with minimal scarring.
Effect of cyproterone acetate on skin surface lipids
Authors: Fanta D , Müller MM
Published: July 2023

Skin surface lipids in female patients with acne were examined before and after treatment with the oral contraceptive SH 209 AB (2 mg cyproterone acetate and 0.05 mg ethinyl estradiol) by direct extraction with petrol ether and photometrical determination. A significant reduction in the skin surface lipids was seen, together with a remarkable alleviation of the disease.
Internal dissemination of mycosis fungoides despite successful local therapy
Authors: Fischer T , Van Vloten WA , Volden G
Published: July 2023

Abstract is not available
DNA interstrand crosslinks visualized by electron microscopy in PUVA-treated psoriasis
Authors: Lerche A , Søndergaard J , Wadskov S , Leick V , Bohr V.
Published: July 2023

An electron microscopic method has been used to visualize crosslinks after total denaturation of DNA isolated from epidermis and dermis in patients with psoriasis treated with 8-methoxypsoralen (8-MOP) and irradiation with ultraviolet light at 360 nm (PUVA treatment). This technique enabled accurate measurement of the number and density of DNA interstrand crosslinks. 30 biopsies were studied and a total of 9503 DNA molecules were scored in the electron microscope. 6 patients were treated topically with 8-MOP and 10 were on systemic treatment and biopsies were obtained immediately after irradiation. 1% of the total DNA molecules examined contained 3 or more crosslinks. The overall frequency of crosslinks was almost identical in the epidermis (1.1%) and in the dermis (0.9%) and, furthermore, virtually the same in patients on topical and systemic PUVA treatment. The total number of crosslinks was of the same magnitude as those previously found by us in normal human skin. Thus, though the formation of interstrand DNA crosslinks can be considered as an indicator of damage of the genetic material, we were unable to demonstrate this as being a consequence of PUVA treatment using 8-MOP.
In situ identification of mononuclear cells infiltrating cutaneous carcinoma: an immuno-histochemical study
Authors: De Panfilis G , Colli V , Manfredi G , Misk I , Rima S , Zampetti M , Allegra F.
Published: July 2023

The present study was undertaken in order to investigate the exact nature of the cells surrounding cutaneous tumours. Formalin-fixed, paraffin-embedded sections were tested with a horse antihuman T lymphocyte serum and with IgG, IgA and IgM rabbit antihuman serum. All the sections were respectively treated with rabbit anti-horse or swine antirabbit peroxidase-labelled serum. The advantages offered by the immunoperoxidase technique are briefly discussed. T cells are in overwhelming proportion in comparison with IgG, IgA and IgM bearing cells. This seems a further demonstration that mononuclear infiltrate surrounding cutaneous carcinoma mainly represents a cell-mediated immune response.
Percutaneous absorption of DDT from a parasiticide used for treatment of scabies
Authors: Kolmodin-Hedman B , Borglund E , Werner Y.
Published: July 2023

Moderately to markedly increased plasma concentrations of DDT and its major metabolite DDE were found in 3 boys 4, 7 and 9 years of age and also in a
Intracellular distribution of dopa and 5-S-cysteinyldopa in pigment cells with minimal pigment formation
Authors: Agrup G , Hansson C , Rorsman H , Rosengren AM , Rosengren E.
Published: July 2023

The hypothesis that only melanosomal catecholic amino acids contribute to melanin formation was tested by studying adult bovine eyes in which pigment synthesis is considered to be low or absent. Dopa and 5-S-cysteinyldopa were investigated in different cell fractions of the choroid and retinal pigment epithelium of cattle. Most of the dopa and 5-S-cysteinyldopa was found in the cytoplasm and very little in the large granule fraction. The presence of cysteinyldopa in the adult eye is evidence of tyrosinase activity, but the catechol amino acids in the cytoplasm probably do not give rise to melanin formation. It is assumed that they instead are excreted from the cells.
Ultrastructure of skin in primary systemic amyloidosis
Authors: Kobayasi T , Asboe-Hansen G.
Published: July 2023

Amyloid masses were found in the dermis of two brothers suffering from primary amyloidosis. The masses consisted of fibrils, composed in turn of twin hollow filaments of amyloid. An amyloid filament appeared as a 3 nm thick lucent core with a 2 nm thick wall. Occasionally 4--6 filaments were packed together in one fibril. The twin filaments were slightly twisted, with a twisting angle of 2.5 degrees and a coiling pitch of 1 160 nm. Wavy shapes of amyloid fibrils were also seen in elastic fibres. Amyloid fibrils were found in elastic fibres, under the basal lamina of the epidermis, sweat gland epithelium and Schwann cells; also around perineural cells, perivascular cells and, in one of the brothers, in collagen fibril bundles. No amyloid fibrils were found under the endothelial basal lamina. It would appear that amyloid fibrils are pathological fibrils belonging to the elastic fibre-basal lamina system.
Electron spin resonance studies on phaeomelanins
Authors: Hansson C , Agrup G , Rorsman H , Rosengren AM , Rosengren E.
Published: July 2023

ESR spectra of synthetic melanins formed from dopa and from 5-S-cysteinyldopa and of melanins isolated from human melanomas were studied. Alkali-soluble melanin synthesized from cysteinyldopa showed a signal similar to that of insoluble melanin synthesized from dopa, but also a simple fine structure. Trichochromes C and F synthesized from 5-S-cysteinyldopa had a broader signal than dopamelanin, and in addition a hyperfine structure that could be explained by the presence of manganous ions. An insoluble eumelanin from a human melanoma with a sulphur content of 5% showed an ESR spectrum consisting of one line like dopamelanin. An alkali-soluble phaeomelanin from a human melanoma with a sulphur content of 10% showed a very broad signal with a hyperfine structure that could be explained by the presence of manganous ions.
Immunoglobulins in psoriasis skin lesions
Authors: Burnham TK.
Published: July 2023

Abstract is not available.
Intracellular collagen fibrils in cultured human skin
Authors: Hentzer B , Kobayasi T.
Published: July 2023

During cultivation, the collagen fibrils of skin explants are broken down. The cells of the explants participate in this resorption. The ultrastructure of the intracellular degradation of collagen fibrils of cultured human skin has been examined. Intracellular collagen fibrils occur in fibroblasts, macrophages, smooth muscle cells and unidentifiable cells. Normal collagen fibrils are engulfed and appear within membrane-bounded tubes of the cytoplasm. Primary lysosomes fuse with the tubes. Degraded intracellular collagen fibrils are frequently present in secondary lysosomes and show decreasing diameters, filamentous splitting, loss of axial periodicity, variable stainability and cross-banded filamentous aggregates. The changes in the intracellular collagen fibrils are identical with those seen in the extracellular space. The present study demonstrates that various cell types in dermis are involved in collagen fibril degradation and that the lysosomes play an important part in the intracellular resorption.
Malignant dermal eccrine cylindroma
Authors: Bondeson L.
Published: July 2023

Abstract is not available.
Scarring alopecia in psoriasis.
Authors: Wright A L , Messenger A G
Published: July 2023

Scalp biopsies were taken from 3 patients with a scarring alopecia associated with severe scalp psoriasis. The histological findings in each case showed inflammatory destruction of the infundibular region of the hair follicle. The similarity of these changes in each case strongly suggests an association with the psoriasis.
On longitudinal melanonychia after healing of lichen planus.
Authors: Juhlin L , Baran R
Published: July 2023

No abstract available.
Correlation between ageing and collagen gel contractility of human fibroblasts.
Authors: Kono T , Tanii T , Furukawa M , Mizuno N , Kitajima J , Ishii M , Hamada T
Published: July 2023

To investigate the influence of ageing on wound healing, we cultured fibroblasts derived from human dermis in type I collagen gel, and evaluated the relationship between gel contractility and ageing. Cells were obtained from children (0-15 years old, Group A), early adulthood (16-40 years old, Group B), mid-adulthood (41-60 years old, Group C), and the elderly (61 or older, Group D). Gel contractility was determined by measuring the diameter on the second day after gel preparation. Within the tenth passage, gel contraction was the most marked in Group A, but did not differ among the other groups. Gel contraction at passages 30-40 did not differ from those within the tenth passage in Groups B, C and D, but it decreased markedly in Group A to a value similar to that in the other groups. These results show that fibroblasts in childhood are more contractile than those in adulthood and are more readily affected by passages (in vitro ageing).
Incontinentia pigmenti in identical twins with separate skin and neurological disorders.
Authors: Tanaka K , Kambe N , Fujita M , Ando Y , Takashima S , Yuasa I
Published: July 2023

Incontinentia pigmenti in female identical twins is reported. The first baby showed the typical pigmentation of incontinentia pigmenti, while the second baby had hydrocephalus (colpocephaly) without pigmentation. They were identical, with a rate of 99.9% in 18 blood-type studies. Virus was not detected and cytogenetic studies proved normal. Both showed peripheral eosinophilia. The individual expressions of Incontinentia pigmenti in these identical twins were separated into cutaneous lesions and lesion of the central nervous system (intra-uterine hydrocephalus). Cutaneous lesions developed after birth. Twins with Incontinentia pigmenti are extremely rare and in this family showed different expressions of this disease in space and time.
Initial hyperproliferation and incomplete terminal differentiation of cultured human keratinocytes from lesional and uninvolved psoriatic skin
Authors: Detmar M , Mayer-da-Silva A , Stadler R , Orfanos C E
Published: July 2023

Human epidermal keratinocytes (KCs) were isolated from lesional and from uninvolved skin of 8 patients with chronic plaque-like psoriasis and from the normal skin of 8 healthy volunteers. Primary KC cultures, grown on 3T3 cell feeder layers, were examined over a period of 4 weeks and their plating efficiency, colony growth area, DNA synthesis and ultrastructural cell differentiation were evaluated. Psoriatic KCs formed colonies one day earlier than non-psoriatic controls and proliferated faster during the first 2 weeks, as assessed by the mean colony growth area and 3H-thymidine incorporation. After 4 weeks, however, no significant differences were observed between the in vitro proliferation parameters of normal and psoriatic KCs. At the ultrastructural level, cultures of lesional psoriatic KCs consisted of more cell layers with adherent transitional cells and incomplete formation of cornified envelopes, even after 4 weeks, while KCs from uninvolved psoriatic skin were characterized by a transient delay of in vitro maturation. These results indicate that the characteristic hyperproliferation of psoriatic KCs may only be maintained over a short period of primary culture, whereas defective terminal differentiation of lesional psoriatic KCs was maintained throughout the culture period.
Antipruritic effect of oral cyclosporin A in atopic dermatitis.
Authors: Wahlgren C F , Scheynius A , Hägermark O
Published: July 2023

The effect of ten days' treatment with cyclosporin A, 5 mg/kg/day, in 10 adults with atopic dermatitis was investigated using a double-blind, randomized, placebo-controlled, cross-over design. Evaluation was based on itch recording, clinical scoring and immunohistochemical examination of skin biopsy specimens. Cyclosporin A significantly reduced the itch intensity, the eczema score and the consumption of topical hydrocortisone. A significant decrease in serum magnesium and in the total number of blood eosinophils was seen. No other laboratory abnormalities were observed. In lesional skin, Cyclosporin A induced a relative decrease of CD3+ T cells in 5/10 patients, of HLA-DR+ cells in 6/10, and of interleukin-2-receptor positive (CD25+) cells in 4/10. However, these changes in phenotype expression did not seem necessary for itch relief. Relapse of clinical symptoms was seen within 2-30 days of completion of the Cyclosporin A course. The mechanism of the antipruritic effect remains unclear, but the present findings may support the hypothesis that 'pruritogenic cytokines', whose production is inhibited by Cyclosporin A, may be important in the pathogenesis of itch in atopic dermatitis.
Reversal of andro-genetic alopecia in a male. A spironolactone effect?
Authors: Bou-Abboud C F , Nemec F , Toffel F
Published: July 2023

This 73-year-old white male has been bald since the age of 28. He developed nonA-nonB-induced liver cirrhosis and had been treated with spironolactone for the last 6 years. For the last 3 months, his hair had started to regrow over the scalp. This might be related to the antiandrogenic effect of spironolactone.
Nickel in nails, hair and plasma from nickel-hypersensitive women.
Authors: Gammelgaard B , Veien N K
Published: July 2023

The concentrations of nickel in finger-nails, toe-nails, hair and plasma from 71 nickel-hypersensitive women and 20 non-hypersensitive women were determined. Nickel concentrations in finger-nails were significantly higher than in toe-nails in both the nickel-hypersensitive group and the control group. Nickel-sensitive women had significantly higher levels of nickel in toe-nails, hair and plasma than had control subjects, whereas there was no significant difference in nickel concentration in finger-nails between the two groups. No correlation could be demonstrated between nickel levels in any combination of nails, hair and plasma in the nickel-hypersensitive or in the control group.