bluuebunny/crossref_metadata_2025 huggingface dataset:
Targeting the Cerebrospinal Fluid Compartment in Glaucoma: Still the Dark Side of the Moon? Comment on Passaro et al. Glaucoma as a Tauopathy—Is It the Missing Piece in the Glaucoma Puzzle? J. Clin. Med. 2023, 12, 6900
Authors: Wostyn Peter
Published: January 2024

I read with great interest the article by Passaro et al [...]
Exploring the Impact of Glycemic Control on Diabetic Retinopathy: Emerging Models and Prognostic Implications
Authors: Tecce Nicola , Cennamo Gilda , Rinaldi Michele , Costagliola Ciro , Colao Annamaria
Published: January 2024

This review addresses the complexities of type 1 diabetes (T1D) and its associated complications, with a particular focus on diabetic retinopathy (DR). This review outlines the progression from non-proliferative to proliferative diabetic retinopathy and diabetic macular edema, highlighting the role of dysglycemia in the pathogenesis of these conditions. A significant portion of this review is devoted to technological advances in diabetes management, particularly the use of hybrid closed-loop systems (HCLSs) and to the potential of open-source HCLSs, which could be easily adapted to different patients’ needs using big data analytics and machine learning. Personalized HCLS algorithms that integrate factors such as patient lifestyle, dietary habits, and hormonal variations are highlighted as critical to reducing the incidence of diabetes-related complications and improving patient outcomes.
Impact of Intercostal Artery Reinsertion on Neurological Outcome after Thoracoabdominal Aortic Replacement: A 25-Year Single-Center Experience
Authors: Helms Florian , Poyanmehr Reza , Krüger Heike , Schmack Bastian , Weymann Alexander , Popov Aron-Frederik , Ruhparwar Arjang , Martens Andreas , Natanov Ruslan
Published: January 2024

Background: Intercostal artery reinsertion (ICAR) during thoracoabdominal aortic replacement remains controversial. While some groups recommend the reinsertion of as many arteries as possible, others consider the sacrifice of multiple intercostals practicable. This study investigates the impact of intercostal artery reinsertion or sacrifice on neurological outcomes and long-term survival after thoracoabdominal aortic repair. Methods: A total of 349 consecutive patients undergoing thoracoabdominal aortic replacement at our institution between 1996 and 2021 were analyzed in a retrospective single-center study. ICAR was performed in 213 patients, while all intercostal arteries were ligated and sacrificed in the remaining cases. The neurological outcome was analyzed regarding temporary and permanent paraplegia or paraparesis. Results: No statistically significant differences were observed between the ICAR and non ICAR groups regarding the cumulative endpoint of transient and permanent spinal cord-related complications (12.2% vs. 11.8%, p = 0.9). Operation, bypass, and cross-clamp times were significantly longer in the ICAR group. Likewise, prolonged mechanical ventilation was more often necessary in the ICAR group (26.4% vs. 16.9%, p = 0.03). Overall long-term survival was similar in both groups in the Kaplan–Meier analysis. Conclusion: Omitting ICAR during thoracoabdominal aortic replacement may reduce operation and cross-clamp times and thus minimize the duration of intraoperative spinal cord hypoperfusion.
A Review of Methods for Modelling Flooding, Its Progression and Outcome in Damaged Ships
Authors: Rodrigues José Miguel
Published: January 2024

The timely and precise prediction of flooding progression and its eventual outcome in ships with breached hulls can lead to dramatic improvements in maritime safety through improved guidance for both emergency response and ship design. The traditional approach to assessing damage-induced flooding in both these stages, which also fully complies with statutory rules, is through static calculations. On the other hand, the application of models that simulate the flooding progression and the behaviour of flooded ships from, or close to, first principles allows for increased accuracy of the modelling of the phenomenon. This increase in accuracy can then be used to support advanced design for safety procedures. Furthermore, it can considerably enhance a ship’s capability for damage identification and inference-based logic for emergency decision support systems and marine accident response in general. This paper conducts a review of selected state-of-the-art methods, procedures, and case studies in recent years which aimed to model progressive flooding and damage ship behaviour and provide some explanations of fundamentals. Applications related to damage identification, the prediction of outcome/situation awareness, and flooding emergency response are also briefly discussed. The paper concludes with a brief reflection on salient gaps in the context of accelerating the development of these methods and their applicability.
Short Sea Shipping in the Age of Sustainability, Autonomous Navigation and Digitalization
Authors: Batista Santos Pedro M. , Santos Tiago A.
Published: January 2024

This paper presents the comprehensive state-of-the-art on the challenges that short sea shipping currently faces across the world. The concept and its relationship with coastal shipping are introduced, followed by a review of the EU policies for short sea shipping and its practical impacts in modal split. A survey of the literature on the strong and weak points of this form of transportation is carried out, aimed at explaining the difficulties in achieving relevant modal shifts from road to sea. The experience with short sea shipping across the world is described and discussed, providing a global perspective. The paper then discusses the main challenges and opportunities in this field, namely decarbonisation, autonomous navigation, and digitalization. Conclusions are drawn on the possible impact of these game changing developments in this segment of the shipping industry.
Deformation Intelligent Prediction of Titanium Alloy Plate Forming Based on BP Neural Network and Sparrow Search Algorithm
Authors: Wang Shun , Wang Jiayan , Xu Zhikang , Wang Ji , Li Rui , Dai Jinliang
Published: January 2024

The application of titanium alloy in shipbuilding can reduce ship weight and carbon emissions. To solve the problem of titanium alloy forming, the deformation prediction of titanium alloy line heating based on a backpropagation (BP) neural network and sparrow search algorithm (SSA) was researched. Based on the thermal–elastic–plastic finite element method, the numerical calculation model of TA5 titanium alloy overlapping heating forming was established. The feasibility of the model was verified by comparing it with the numerical calculation and experiment of low-carbon steel. Considering the characteristics of the titanium alloy-forming process, 73 groups of titanium alloy-forming schemes were obtained by the Latin hypercube sampling method. The deformation data of the samples were obtained by using the numerical calculation model of titanium alloy forming. The prediction methods of titanium alloy-forming deformation based on BP, genetic algorithm–backpropagation (GA-BP), and SSA-BP were proposed. The accuracy of different neural network prediction models was analyzed. The mean absolute percentage errors (MAPEs) of BP, GA-BP, and SSA-BP in shrinkage prediction were 7.45%, 4.08%, and 2.96%, respectively. The MAPEs of BP, GA-BP, and SSA-BP in deflection prediction were 8.44%, 4.73%, and 2.64%, respectively. The goodness of fit (R2) of SSA-BP is closest to 1 among the three models. The calculation results show that SSA-BP is better than BP and GA-BP in predicting the forming deformation of titanium alloy. The maximum prediction error of SSA-BP is 4.95%, which is within the allowable range of engineering error. The SSA-BP prediction model is suitable for the rapid and accurate prediction of the deformation of titanium alloy line heating forming. The intelligent prediction model provides data support for intelligent decisions for titanium alloy forming.
A Comparative Study on the Structural Response of Multi-Linked Floating Offshore Structure between Digital Model and Physical Model Test for Digital Twin Implementation
Authors: Sim Kichan , Lee Kangsu
Published: January 2024

A digital twin is a virtual model of a real-world structure (such as a device or equipment) which supports various problems or operations that occur throughout the life cycle of the structure through linkage with the actual structure. Digital twins have limitations as a general simulation method because the characteristic changes (motion, stress, vibration, etc.) that occur in the actual structure must be acquired through installed sensors. Additionally, it takes a huge computing cost to output changes in the structure’s characteristics in real time. In particular, in the case of ships and offshore structures, simulation requires a lot of time and resources due to the size of the analysis model and environmental conditions where the wave load acts irregularly, so the application of a different simulation methodology from existing ones is required. The order reduction method, which accurately represents the system’s characteristics and expresses them in a smaller model, can significantly reduce analysis time and is an effective option. In this study, to analyze the applicability of the order reduction method to the development of digital twins for offshore structures, the structural responses of a multi-connected floating offshore structure were estimated by applying the order reduction method based on distortion base mode. The order reduction method based on the distortion base mode predicts the responses by constructing an order-reduced conversion matrix consisting of the selected distortion base mode, based on the mode vector’s orthogonality and autocorrelation coefficients. The predicted structural responses with the reduced order model (ROM) were compared with numerical analysis results derived using the higher order boundary element method and finite element method with in-house code owned by the Korea Research Institute of Ship & Ocean Engineering and measured responses with a model test. When compared with the numerical analysis results, the structural responses were predicted with high accuracy in the wave direction and wave frequency band of the selected distortion base mode, but there are differences due to changed characteristics of the structure when compared with the results of the model test. In addition, differences were also seen in reduced order model evaluation with different sensor locations, and it was confirmed that the more similar the extracted distortion base modes of input sensor location set is to the distortion base modes of predicted location set, the higher accuracy is in predicting the structural responses. As a result, the performance of the reduced order model is determined by the distortion base mode selection method, the locations of the sensor, and the prediction for the structural response.
Molecular Study of Pneumocystis jirovecii in Respiratory Samples of HIV Patients in Chile
Authors: Iturrieta-González Isabel , Chahin Carolina , Cabrera Johanna , Concha Carla , Olivares-Ferretti Pamela , Briones Javier , Vega Fernando , Bustos-Medina Luis , Fonseca-Salamanca Flery
Published: January 2024

Pneumocystis is an opportunistic fungus that causes potentially fatal pneumonia (PCP) in immunocompromised patients. The objective of this study was to determine the prevalence of P. jirovecii in HIV patients through phenotypic and molecular study, to investigate the genetic polymorphisms of P. jirovecii at the mitochondrial gene mtLSU and at the nuclear dihydropteroate synthase gene (DHPS), and by analysis of molecular docking to study the effect of DHPS mutations on the enzymatic affinity for sulfamethoxazole. A PCP prevalence of 28.3% was detected, with mtLSU rRNA genotypes 3 (33.3%) and 2 (26.6%) being the most common. A prevalence of 6.7% (1/15) mutations in the DHPS gene was detected, specifically at codon 55 of the amino acid sequence of dihydropteroate synthase. Molecular docking analysis showed that the combination of mutations at 55 and 98 codons is required to significantly reduce the affinity of the enzyme for sulfamethoxazole. We observed a low rate of mutations in the DHPS gene, and molecular docking analysis showed that at least two mutations in the DHPS gene are required to significantly reduce the affinity of dihydropteroate synthase for sulfamethoxazole.
Exposure and Health Risks Posed by Potentially Toxic Elements in Soils of Metal Fabrication Workshops in Mbarara City, Uganda
Authors: Nuwamanya Eunice , Byamugisha Denis , Nakiguli Caroline K. , Angiro Christopher , Khanakwa Alice V. , Omara Timothy , Ocakacon Simon , Onen Patrick , Omoding Daniel , Opio Boniface , Nimusiima Daniel , Ntambi Emmanuel
Published: January 2024

Metal fabrication workshops (MFWs) are common businesses in Ugandan cities, and especially those producing metallic security gates, window and door frames (burglar-proof), and balcony and staircase rails. The objective of this study was to comparatively assess the pollution levels and potential health risks of manganese (Mn), chromium (Cr), cadmium (Cd), lead (Pd) and nickel (Ni) in pooled surface soil samples from four 5-, 7-, 8-, and 10-year-old MFWs (n = 28) and a control site (n = 8) in Mbarara City, Uganda. The concentration of the potentially toxic elements (PTEs) was determined using inductively coupled plasma–optical emission spectrometry. Contamination, ecological, and human health risk assessment indices and models were used to identify any risks that the PTEs could pose to the pristine environment and humans. Our results showed that PTE pollution of soils is occuring in the MFWs than at the control site. The mean concentrations of the PTEs (mg kg−1) in the samples were: Mn (2012.75 ± 0.23–3377.14 ± 0.31), Cr (237.55 ± 0.29–424.93 ± 0.31), Cd (0.73 ± 0.13–1.29 ± 0.02), Pb (107.80 ± 0.23–262.01 ± 0.19), and Ni (74.85 ± 0.25–211.37 ± 0.14). These results indicate that the PTEs could plausibly derive from the fabrication activities in these workshops, which is supported by the high values of contamination factors, index of geoaccumulation, and the overall increase in pollution load indices with the number of years of operation of the MFWs. Human health risk assessment showed that there are non-carcinogenic health risks that could be experienced by children who ingest PTEs in the soils from the 7-, 8- and 10-year-old MFWs. The incremental life cancer risk assessment suggested that there are potential cancerous health effects of Cd and Ni that could be experienced in children (who ingest soils from all the four MFWs) and adults (ingesting soils from the 8- and 10-year-old MFWs). This study underscores the need to implement regulatory guidelines on the operation and location of MFWs in Uganda. Further research should be undertaken to investigate the emission of the PTEs during welding operations in the MFWs.
Psoriasis and Fibromyalgia: A Systematic Review
Authors: D’Onghia Martina , Ursini Francesco , Cinotti Elisa , Calabrese Laura , Tognetti Linda , Cartocci Alessandra , Lazzeri Laura , Frediani Bruno , Rubegni Pietro , Trovato Emanuele
Published: January 2024

Psoriasis is a chronic inflammatory cutaneous condition characterized by several comorbidities, including musculoskeletal disorders. While the association with psoriatic arthritis has been widely addressed in literature, the aim of the present systematic review was to identify all available evidence on the relationship between psoriasis and fibromyalgia, a musculoskeletal syndrome primarily characterized by chronic widespread pain. We followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, and MedLine and Web of Science (WOS) databases were searched for literature up to March 2023. After the removal of duplicate records, a total of 11 articles were deemed eligible for inclusion in a qualitative synthesis. Our results suggested that psoriatic patients had a higher prevalence of fibromyalgia (8–30%), with a very high impact on symptoms of psoriasis. Moreover, fibromyalgic patients had a slightly increased prevalence of psoriasis (2.2–6.7%) compared to the control groups. Finally, several studies demonstrated the substantial impact of fibromyalgia on psoriatic outcome measures in patients with concomitant psoriatic arthritis. In conclusion, available data support a potential interplay between psoriasis and fibromyalgia, but further research is encouraged in this area.
Folate Receptor Alpha Autoantibodies in the Pediatric Acute-Onset Neuropsychiatric Syndrome (PANS) and Pediatric Autoimmune Neuropsychiatric Disorders Associated with Streptococcal Infections (PANDAS) Population
Authors: Wells Lindsey , O’Hara Nancy , Frye Richard , Hullavard Neeshi , Smith Erica
Published: January 2024

The folate receptor alpha autoantibodies (FRAAs) are associated with cerebral folate deficiency (CFD) and autism spectrum disorder (ASD). Both of these syndromes have overlapping characteristics with Pediatric Autoimmune Neuropsychiatric Disorders Associated with Streptococcal Infections (PANDAS) and Pediatric Acute-Onset Neuropsychiatric Syndrome (PANS). Thus, we propose that the FRAAs may contribute to the symptomatology of PANS/PANDAS. To test this hypothesis, 1 mL of serum from 47 patients (age range = 6–18 years old) clinically diagnosed with PANS/PANDAS was sent to Vascular Strategies (Plymouth Meeting, PA, USA) for analysis of FRAAs. Moreover, 63.8% of PANS/PANDAS patients (male = 15; female = 15) were found to have either the blocking and/or blinding FRAAs, with 25 (83.3%; male = 14; female = 11) having binding FRAAs, two (6.7%; all female = 2) having blocking FRAAs, and 3 (10%; male = 1; female = 2) having both binding and blocking. Furthermore, surprisingly, ASD was associated with a 0.76 lower binding titer (p = 0.02), and severe tics were associated with a 0.90 higher binding titer (p = 0.01). A case of a FRAA-positive patient is provided to illustrate that a treatment plan including leucovorin can result in symptom improvement in patients with PANS/PANDAS who are FRAA-positive. These data, for the first time, demonstrate that PANS/PANDAS is associated with FRAAs and suggest folate metabolism abnormalities may contribute to PANS/PANDAS symptomatology. Further studies investigating the therapeutic nature of leucovorin in the treatment of PANS/PANDAS are needed. Such studies may open up an alternative, safe, and well-tolerated treatment for those with the PANS/PANDAS diagnosis.
Correlation between Carboxyhemoglobin Levels Measured by Blood Gas Analysis and by Multiwave Pulse Oximetry
Authors: Yu Jisu , Lee Juncheol , Cho Yongil , Oh Jaehoon , Kang Hyunggoo , Lim Tae Ho , Ko Byuk Sung
Published: January 2024

Carbon monoxide (CO) poisoning is difficult to diagnose owing to its nonspecific symptoms. Multiwave pulse oximetry can be used to quickly screen patients for CO poisoning. However, few studies have analyzed patients with CO poisoning who presented to the emergency department (ED). The primary aim of our study was to determine the correlation between COHb levels measured in blood gas analysis and COHb levels measured in multiwave pulse oximetry. Secondary aims were the sensitivity and specificity of the COHb level cutoff value using multiwave pulse oximetry to predict a 25% COHb level in blood gas analysis. This single-center retrospective observational study included patients with CO poisoning who visited the ED of a university-affiliated hospital in Seoul, Korea between July 2021 and June 2023. COHb poisoning was determined using blood gas analysis and multiwave pulse oximetry. The correlation of COHb levels between the two tests was evaluated using correlation analysis. The area under the receiver operating characteristic curve (AUC) of multiwave pulse oximetry was calculated to predict COHb levels from the blood gas analysis. The optimal cutoff values, sensitivity, and specificity of COHb were determined. A total of 224 patients who had COHb levels measured using both multiwave pulse oximetry and blood gas analysis were included in the analysis. In the correlation analysis, COHb showed a high positive correlation with COHb measured using blood gas analysis (Spearman correlation coefficient = 0.86, p < 0.001). The AUC of COHb measured by multiwave pulse oximetry to predict 25% of the COHb level (which can be an indication of hyperbaric oxygen treatment) measured by blood gas analysis was 0.916. When the COHb levels measured with multiwave pulse oximetry were 20% the sensitivity was 81% and the specificity was 83%, and when the COHb levels were 25% the sensitivity was 50% and the specificity was 95%. The COHb value measured using multiwave pulse oximetry blood gas analysis showed a high correlation. However, additional research using large-scale studies is required for validation.
Stroke after Cardiac Surgery: A Risk Factor Analysis of 580,117 Patients from UK National Adult Cardiac Surgical Audit Cohort
Authors: Asta Laura , Falco Daniele , Benedetto Umberto , Porreca Annamaria , Majri Fatma , Angelini Gianni , Sensi Stefano , Di Giammarco Gabriele
Published: January 2024

Cerebrovascular accident is the most ominous complication observed after cardiac surgery, carrying an increased risk of morbidity and mortality. Analysis of the problem shows its multidimensional nature. In this study, we aimed to identify major determinants among classic variables, either demographic, clinical or type of surgical procedure, based on the analysis of a large dataset of 580,117 patients from the UK National Adult Cardiac Surgical Audit (NACSA). For this purpose, univariate and multivariate logistic regression models were utilized to determine associations between predictors and dependent variable (Stroke after cardiac surgery). Odds ratios (ORs) and 95% confidence intervals (CIs) were constructed for each independent variable. Statistical analysis allows us to confirm with greater certainty the predictive value of some variables such as age, gender, diabetes mellitus (diabetes treated with insulin OR = 1.37, 95%CI = 1.23–1.53), and systemic arterial hypertension (OR = 1.11, 95%CI = 1.05–1.16);, to emphasize the role of preoperative atrial fibrillation (OR = 1.10, 95%CI = 1.03–1.16) extracardiac arteriopathy (OR = 1.70, 95%CI = 1.58–1.82), and previous cerebral vascular accident (OR 1.71, 95%CI = 1.6–1.9), and to reappraise others like smoking status (crude OR = 1.00, 95%CI = 0.93–1.07 for current smokers) or BMI (OR = 0.98, 95%CI = 0.97–0.98). This could allow for better preoperative risk stratification. In addition, identifying those surgical procedures (for example thoracic aortic surgery associated with a crude OR of 3.72 and 95%CI = 3.53–3.93) burdened by a high risk of neurological complications may help broaden the field of preventive and protective techniques.
Possible Role of Fibrinaloid Microclots in Postural Orthostatic Tachycardia Syndrome (POTS): Focus on Long COVID
Authors: Kell Douglas B. , Khan Muhammed Asad , Kane Binita , Lip Gregory Y. H. , Pretorius Etheresia
Published: January 2024

Postural orthostatic tachycardia syndrome (POTS) is a common accompaniment of a variety of chronic, inflammatory diseases, including long COVID, as are small, insoluble, ‘fibrinaloid’ microclots. We here develop the argument, with accompanying evidence, that fibrinaloid microclots, through their ability to block the flow of blood through microcapillaries and thus cause tissue hypoxia, are not simply correlated with but in fact, by preceding it, may be a chief intermediary cause of POTS, in which tachycardia is simply the body’s exaggerated ‘physiological’ response to hypoxia. Similar reasoning accounts for the symptoms bundled under the term ‘fatigue’. Amyloids are known to be membrane disruptors, and when their targets are nerve membranes, this can explain neurotoxicity and hence the autonomic nervous system dysfunction that contributes to POTS. Taken together as a system view, we indicate that fibrinaloid microclots can serve to link POTS and fatigue in long COVID in a manner that is at once both mechanistic and explanatory. This has clear implications for the treatment of such diseases.
Ensuring Financial System Sustainability: Combating Hybrid Threats through Anti-Money Laundering and Counter-Terrorist Financing Measures
Authors: Korauš Antonín , Jančíková Eva , Gombár Miroslav , Kurilovská Lucia , Černák Filip
Published: January 2024

This paper deals with ensuring the sustainability of the financial system and combating hybrid threats in relation to anti-money laundering and counter-terrorist financing (AML/CTF) measures. International cooperation in the field of combating hybrid threats is only at the beginning, and in many ways, the experience of international cooperation in the fight against money laundering and terrorist financing, which is based on many years of experience in the institutional and legislative fields, could be used. Hybrid threats are constantly changing and evolving, which means our response to them must also constantly evolve and adapt. The aim of the presented study is the analysis of the problem of the legalization of income from criminal activity and the financing of terrorism and their possible relationship with the fight against hybrid threats and maintaining the stability of the financial system.
SENOSI Confocal Microscopy: A New and Innovating Way to Detect Positive Margins in Non-Palpable Breast Cancer?
Authors: Wernly Deborah , Beniere Charles , Besse Valerie , Seidler Stephanie , Lachat Regine , Letovanec Igor , Huber Daniela , Simonson Colin
Published: January 2024

In Switzerland, breast cancer is the leading cancer among women, with breast-conserving surgery (BCS) being the preferred treatment for small tumors. The margin status post-surgery is a critical predictor of local recurrence. Achieving negative margins remains a challenge, leading to re-excision in 20–30% of cases. Traditional methods like intraoperative examination palpation and radiography have limitations in assessing excised margins. This study introduces the Histolog® Scanner, a confocal microscopy tool, as a potential solution. It provides real-time images of tissue architecture, allowing for rapid and accurate assessment of excised margins. Our research compared the Histolog® Scanner with standard per-operative radiography in patients with non palpable breast cancer. Preliminary results indicate that the Histolog® Scanner offers a reliable and time-efficient method for margin assessment, suggesting its potential for clinical integration.
Second Trimester Amniotic Fluid Angiotensinogen Levels Linked to Increased Fetal Birth Weight and Shorter Gestational Age in Term Pregnancies
Authors: Vrachnis Dionysios , Fotiou Alexandros , Mantzou Aimilia , Pergialiotis Vasilios , Antsaklis Panagiotis , Valsamakis George , Stavros Sofoklis , Machairiotis Nikolaos , Iavazzo Christos , Kanaka-Gantenbein Christina , Mastorakos George , Drakakis Petros , Vrachnis Nikolaos , Antonakopoulos Nikolaos
Published: January 2024

Background: Despite the considerable progress made in recent years in fetal assessment, the etiology of fetal growth disturbances is not as yet well understood. In an effort to enhance our knowledge in this area, we investigated the associations of the amniotic fluid angiotensinogen of the renin–angiotensin system with fetal growth abnormalities. Methods: We collected amniotic fluid samples from 70 pregnant women who underwent amniocentesis during their early second trimester. Birth weight was documented upon delivery, after which the embryos corresponding to the respective amniotic fluid samples were categorized into three groups as follows: small for gestational age (SGA), appropriate for gestational age (AGA), and large for gestational age (LGA). Amniotic fluid angiotensinogen levels were determined by using ELISA kits. Results: Mean angiotensinogen values were 3885 ng/mL (range: 1625–5375 ng/mL), 4885 ng/mL (range: 1580–8460 ng/mL), and 4670 ng/mL (range: 1995–7250 ng/mL) in the SGA, LGA, and AGA fetuses, respectively. The concentrations in the three groups were not statistically significantly different. Although there were wide discrepancies between the mean values of the subgroups, the large confidence intervals in the three groups negatively affected the statistical analysis. However, multiple regression analysis revealed a statistically significant negative correlation between the angiotensinogen levels and gestational age and a statistically significant positive correlation between the birth weight and angiotensinogen levels. Discussion: Our findings suggest that fetal growth abnormalities did not correlate with differences in the amniotic fluid levels of angiotensinogen in early second trimester pregnancies. However, increased angiotensinogen levels were found to be consistent with a smaller gestational age at birth and increased BMI of neonates.
Production of Proliferation- and Differentiation-Competent Porcine Myoblasts for Preclinical Studies in a Porcine Large Animal Model of Muscular Insufficiency
Authors: Knoll Jasmin , Amend Bastian , Abruzzese Tanja , Harland Niklas , Stenzl Arnulf , Aicher Wilhelm K.
Published: January 2024

Muscular insufficiency is observed in many conditions after injury, chronic inflammation, and especially in elderly populations. Causative cell therapies for muscle deficiencies are not state of the art. Animal models to study the therapy efficacy are, therefore, needed. We developed an improved protocol to produce myoblasts suitable for pre-clinical muscle therapy studies in a large animal model. Myoblasts were isolated from the striated muscle, expanded by employing five different protocols, and characterized on transcript and protein expression levels to determine procedures that yielded optimized regeneration-competent myoblasts and multi-nucleated myotubes. We report that swine skeletal myoblasts proliferated well under improved conditions without signs of cellular senescence, and expressed significant levels of myogenic markers including Pax7, MyoD1, Myf5, MyoG, Des, Myf6, CD56 (p ≤ 0.05 each). Upon terminal differentiation, myoblasts ceased proliferation and generated multi-nucleated myotubes. Injection of such myoblasts into the urethral sphincter complex of pigs with sphincter muscle insufficiency yielded an enhanced functional regeneration of this muscle (81.54% of initial level) when compared to the spontaneous regeneration in the sham controls without myoblast injection (67.03% of initial level). We conclude that the optimized production of porcine myoblasts yields cells that seem suitable for preclinical studies of cell therapy in a porcine large animal model of muscle insufficiency.
Nanostructure of Superlubricating Tribofilm Based on Friction-Induced a-C:H Films under Various Working Conditions: A Review of Solid Lubrication
Authors: Yin Xuan , Mu Linyuan , Jia Zihang , Pang Haosheng , Chai Chunpeng , Liu Huan , Liang Chang , Zhang Bing , Liu Dameng
Published: January 2024

Diamond-like carbon (DLC) film has gained widespread popularity as a versatile and important solid lubricant material in the field of tribology. Among various types of DLC films, hydrogen-rich DLC (a-C:H) film as a high-performance material has greatly enhanced anti-friction and anti-wear. However, despite its remarkable capabilities, the surface chemical properties and tribological performance of a-C:H film are significantly influenced by the surrounding environment, in special atmospheric conditions. Its super-slip mechanism involves the participation of hydrogen atoms, which can weaken the normal electron number of the outermost layer of a-C:H film. What is more, it is essential to investigate tribofilms in a vacuum or inert gas environment to ascertain the appropriate tribological properties of a-C:H film, which helps in mitigating oxidation effects. When non-doped DLC films are subjected to friction in a dry nitrogen or argon environment, they create sp3-C-rich transfer films on the contact surface, resulting in macroscopic super-slip effects. This paper aims to introduce and discuss the diverse nanostructures of in situ tribofilms in a-C:H film, focusing on the working environment, and explore the prospective application directions of a-C:H film.
Study on the Wear Performance and Wear Prediction of Leaf Spring Calipers under Lubricating Medium Conditions
Authors: Wang Hao , Ding Lei , Zhao Chengfei , Gao Xi , Zhou Jing
Published: January 2024

Leaf spring calipers are a kind of pipe detector that installs strain gauges on the detecting arm, and the strain gauges measure the geometrical dimensions of the inner wall of the pipe by detecting the bending strain of the leaf spring and the sensors of the leaf spring caliper are set up on the detecting arm, so it has higher detecting accuracy and smaller structural dimensions. Leaf spring calipers are widely used because of their outstanding advantages, but their detection arms are worn out, and their detection accuracy increases with the detection distance. In this paper, we establish a wear model of the detection arm for the operation of the leaf spring caliper in crude oil and refined product pipelines, and according to the model, we build a wear test system for the detection arm. The wear test system of the inspection arm simulates the wear between the inspection arm made of G61500 (UNIFIED NUMBERING SYSTEM) material and the pipe made of X80 (API SPEC 5L) material. The wear pattern of the inspection arm in crude oil and refined oil pipelines is investigated by adding lubricating media with similar physical parameters to crude oil and refined oil, such as light mineral oil, SAE 5W-30 lubricant, 600XP 680 lubricant. The experimental results are analyzed to explore the wear performance of the leaf spring caliper arm, and the prediction algorithm is used to predict the wear pattern of the leaf spring after lubrication. The results show that the average error between the predicted and actual values meets the accuracy requirements, and the wear prediction model of the detection arm can be used as a correction algorithm for the wear error of the leaf spring caliper to improve the detection accuracy.
Study on the Characteristics of Circumferential and Longitudinal Flow of Vault Concrete during Tunnel Lining Pouring Processes
Authors: Yang Shuai , Wu Yimin , Zhou Zhuangzhuang
Published: January 2024

With a large number of railroad and highway tunnels opening for operation, the diseases caused by hidden lining defects are increasing. The study of flow characteristics of freshly mixed concrete during tunnel lining casting is the key to revealing the formation mechanism of hidden defects. This paper revealed the location of blank lining formation by investigating the circumferential and longitudinal flow characteristics of concrete in the vault during tunnel pouring to provide suggestions for improving the quality of tunnel lining pouring for the various projects. This paper adopted the method of indoor testing, selected the suitable working conditions and flow parameters, validated the accuracy of the test with a numerical simulation, and simulated the secondary lining pouring process of the tunnel arch from the circumferential direction and longitudinal direction. This revealed the flow characteristics of the freshly mixed concrete in the process of pouring the arch lining. The flow of concrete in the arch lining was basically characterized by two major features which were similar to the flow in the pumping pipe and the layered flow. It also revealed the relationship between the concrete flow rate, flow distance, and the location of the formation of the blank lining risk zone with the slump of the concrete, the pumping pressure, and the radius of the tunnel.
Low-Velocity Impact and Post-Impact Residual Flexural Properties of Kevlar/EP Three-Dimensional Angle-Interlock Composites
Authors: Shi Juanjuan , Guo Yanwen , Huang Xiaomei , Chen Hongxia , Cao Haijian
Published: January 2024

In this study, five three-dimensional angle-interlock fabrics with different warp and weft densities were fabricated using 1000D Kevlar filaments. The Kevlar/EP composites were prepared by vacuum-assisted molding techniques. The low-velocity impact property of the composite was tested, focusing on the effects of the warp and weft densities, impact energy, impactor shape, and impactor diameter. The damage area, dent depth, and crack lengths in the warp and weft direction were used to evaluate the impact performance, and the specimens were compared with plain-weave composites with similar areal densities. The dominant failure mode of the conical impactor was fiber fracture, while the dominant failure mode of the hemispherical impactor was fiber–resin debonding. The cylindrical impactor showed only minor resin fragmentation. The residual flexural strength of the composite after impact was tested to provide insights into its mechanical properties. The study findings will provide a theoretical basis for the optimization of the design of impact-resistant structures using such materials and facilitate their engineering applications.
Comparing Torque Transmission of Different Bracket Systems in Combination with Various Archwires Considering Play in the Bracket Slot: An In Vitro Study
Authors: Wichelhaus Andrea , Guggenbühl Simon , Hötzel Linus , Seidel Corinna L. , Sabbagh Hisham , Hoffmann Lea
Published: January 2024

This study aims to examine the play between various archwires and bracket systems, exploring potential variations in angle values for specific torque and torque values for a given angle along different bracket systems. Therefore, seven brackets systems were evaluated in conjunction with different stainless steel archwires of varying dimensions (0.016″ × 0.022″, 0.018″ × 0.025″, and 0.019″ × 0.025″). Biomechanical behavior during torque development and transmission was assessed using a six-component force/torque sensor. Torque angles (5–45°) were specified with subsequent torque measurement, and the sequence was reversed by setting the torque (5–30 Nmm) and measuring the angle. A reference measurement with 0 Nmm torque served to evaluate bracket slot play. Bracket play (0 Nmm) during palatal load ranged between 20.06° and 32.50° for 0.016″ × 0.022″ wire, 12.83° and 21.11° for 0.018″ × 0.025″ wire, and 8.39° and 18.73° for 0.019″ × 0.025″ wire. The BioQuick® bracket exhibited the highest play, while Wave SL® and Damon® Q brackets demonstrated the lowest play (p < 0.001). Significant differences (p < 0.001) between the brackets were observed in the torque angles required to achieve torques of 5–20 Nmm. In summary, each bracket system has a different torque transmission, which is of great clinical importance in order to achieve correct torque transmission and avoid complications such as root resorption.
Microstructure and Mechanical Properties of a Novel Al-Mg-Sc-Ti Alloy Fabricated by Laser Powder Bed Fusion
Authors: Shu Zhiheng , Liu Yunzhong
Published: January 2024

(TiH2 + ScH3)/Al-Mg composite powders with different Ti contents were produced by ball milling. These composite powders were fabricated to cube and cuboid shape samples via a laser powder bed fusion process with optimal processing parameters. The TiH2 and ScH3 particles underwent dehydrogenation during the laser powder bed fusion process, and these composite powders ultimately formed Al-Mg-Sc-Ti alloys. The relative density, printability, microstructure, hardness and tensile properties of these alloy samples were investigated. The results show that these Al-Mg-Sc-Ti alloys have lower hot-crack sensitivity, having fine equiaxed grains. An Al18Mg3(Ti,Sc)2 intermetallic phase and in situ L12-Al3(Sc,Ti) precipitations formed during the laser powder bed fusion process, which is beneficial for nucleation and dispersion strengthening. The ultimate tensile strength of the Al-Mg-0.7Sc-1.0Ti alloy was 313.6 MPa with an elongation of 6.6%. During the hot isostatic pressing treatment, most of the Mg element precipitated from the matrix and changed the Al3(Sc,Ti) into a Al18Mg3(Ti,Sc)2 precipitate completely. The Al-Mg-Sc-Ti alloys were nearly fully dense after the hot isostatic pressing treatment and exhibited better mechanical properties. The ultimate tensile strength of the Al-Mg-0.7Sc-1.0Ti was 475 MPa with an elongation of 8.5%.
Sensitivity Analysis on Hyperprior Distribution of the Variance Components of Hierarchical Bayesian Spatiotemporal Disease Mapping
Authors: Jaya I Gede Nyoman Mindra , Kristiani Farah , Andriyana Yudhie , Chadidjah Anna
Published: January 2024

Spatiotemporal disease mapping modeling with count data is gaining increasing prominence. This approach serves as a benchmark in developing early warning systems for diverse disease types. Spatiotemporal modeling, characterized by its inherent complexity, integrates spatial and temporal dependency structures, as well as interactions between space and time. A Bayesian approach employing a hierarchical structure serves as a solution for spatial model inference, addressing the identifiability problem often encountered when utilizing classical approaches like the maximum likelihood method. However, the hierarchical Bayesian approach faces a significant challenge in determining the hyperprior distribution for the variance components of hierarchical Bayesian spatiotemporal models. Commonly used distributions include logGamma for log inverse variance, Half-Cauchy, Penalized Complexity, and Uniform distribution for hyperparameter standard deviation. While the logGamma approach is relatively straightforward with faster computing times, it is highly sensitive to changes in hyperparameter values, specifically scale and shape. This research aims to identify the most optimal hyperprior distribution and its parameters under various conditions of spatial and temporal autocorrelation, as well as observation units, through a Monte Carlo study. Real data on dengue cases in West Java are utilized alongside simulation results. The findings indicate that, across different conditions, the Uniform hyperprior distribution proves to be the optimal choice.
Homological Landscape of Human Brain Functional Sub-Circuits
Authors: Duong-Tran Duy , Kaufmann Ralph , Chen Jiong , Wang Xuan , Garai Sumita , Xu Frederick H. , Bao Jingxuan , Amico Enrico , Kaplan Alan D. , Petri Giovanni , Goni Joaquin , Zhao Yize , Shen Li
Published: January 2024

Human whole-brain functional connectivity networks have been shown to exhibit both local/quasilocal (e.g., a set of functional sub-circuits induced by node or edge attributes) and non-local (e.g., higher-order functional coordination patterns) properties. Nonetheless, the non-local properties of topological strata induced by local/quasilocal functional sub-circuits have yet to be addressed. To that end, we proposed a homological formalism that enables the quantification of higher-order characteristics of human brain functional sub-circuits. Our results indicate that each homological order uniquely unravels diverse, complementary properties of human brain functional sub-circuits. Noticeably, the H1 homological distance between rest and motor task was observed at both the whole-brain and sub-circuit consolidated levels, which suggested the self-similarity property of human brain functional connectivity unraveled by a homological kernel. Furthermore, at the whole-brain level, the rest–task differentiation was found to be most prominent between rest and different tasks at different homological orders: (i) Emotion task (H0), (ii) Motor task (H1), and (iii) Working memory task (H2). At the functional sub-circuit level, the rest–task functional dichotomy of the default mode network is found to be mostly prominent at the first and second homological scaffolds. Also at such scale, we found that the limbic network plays a significant role in homological reconfiguration across both the task and subject domains, which paves the way for subsequent investigations on the complex neuro-physiological role of such network. From a wider perspective, our formalism can be applied, beyond brain connectomics, to study the non-localized coordination patterns of localized structures stretching across complex network fibers.
Multi-Agent Collaborative Rumor-Debunking Strategies on Virtual-Real Network Layer
Authors: Zhong Xiaojing , Zheng Yawen , Xie Junxian , Xie Ying , Peng Yuqing
Published: January 2024

In the era of self-media, the spontaneity and anonymity of information dissemination have led to a surge in rumors, posing significant challenges to cybersecurity. This paper introduces a novel dual-layer VR−SHI1I2R rumor control model for studying collaborative rumor-debunking efforts. Utilizing mathematical modeling and simulation methods, we propose key thresholds for rumor propagation from both theoretical and simulation perspectives, and explore optimal methods for rumor control. Our model is validated with real data from actual cases, confirming its accuracy and the effectiveness. The study shows that without intervention, rumors will spread rapidly. Both constant and dynamically optimized control significantly slow down the spread of rumors. However, dynamic optimization control significantly reduces control costs compared to fixed control schemes. Moreover, we find that controlling only the media layer is insufficient. These findings highlight the importance of meticulous approaches to rumor control in the digital age.
Mortality in IgA Nephropathy: A Long-Term Follow-Up of an Eastern European Cohort
Authors: Ștefan Gabriel , Zugravu Adrian , Stancu Simona
Published: January 2024

Background and Objectives: IgA nephropathy (IgAN), the most common primary glomerulonephritis, has been extensively studied for renal outcomes, with limited data on patient survival, particularly in Eastern Europe. We aimed to investigate the long-term survival rate of patients with IgAN and the associated risk factors in an Eastern European cohort. Materials and Methods: We conducted a retrospective analysis of 215 IgAN patients (median age 44, 71% male) diagnosed at a Romanian tertiary center between 2010 and 2017. We assessed clinical and pathological attributes, including the Charlson comorbidity index, the prevalence of diabetes, renal function, and treatment with renin-angiotensin-system inhibitors (RASIs). Results: Over a median 7.3-year follow-up, 20% of patients died, mostly due to cardiovascular diseases. Survival rates at 1, 5, and 10 years were 93%, 84%, and 77%, respectively. Deceased patients had higher Charlson comorbidity index scores, greater prevalence of diabetes, and poorer renal function. They were less frequently treated with RASIs and more frequently reached end-stage kidney disease (ESKD). Conclusions: We report a 20% mortality rate in our Eastern European IgAN cohort, primarily due to cardiovascular diseases. Death correlates with increased age, comorbidity burden, decreased renal function at diagnosis, and the absence of RASI use. RASI treatment may potentially improve survival, highlighting its importance in managing IgAN.