bluuebunny/crossref_metadata_2025 huggingface dataset:
Kiwifruit Vine Decline Syndrome (KVDS) Alters Soil Enzyme Activity and Microbial Community
Authors: Bergamaschi Valentino , Vera Alfonso , Pirone Lucia , Siles José A. , López-Mondéjar Rubén , Luongo Laura , Vitale Salvatore , Reverberi Massimo , Infantino Alessandro , Bastida Felipe
Published: November 2024

Kiwifruit Vine Decline Syndrome (KVDS) has become a major concern in Italy, impacting both plant health and production. This study aims to investigate how KVDS affects soil health indicators and the composition of soil microbial communities by comparing symptomatic and asymptomatic areas in two kiwifruit orchards located in Latium, Italy. Soil samples were collected during both spring and autumn to assess seasonal variations in soil physicochemical properties, enzyme activities, and microbial biomass. The results reveal that KVDS influences several soil properties, including pH, electrical conductivity, and the contents of water-soluble carbon and nitrogen. However, these effects varied between orchards and across different seasons. Additionally, KVDS significantly impacts soil enzyme activities and microbial biomass, as assessed through the phospholipid fatty acid (PLFA) analysis, particularly showing an increase in fungal biomass in symptomatic areas. Metabarcoding further demonstrates that microbial communities differ between symptomatic and asymptomatic soils, exhibiting notable shifts in both diversity and relative abundance. Our findings emphasise the complex interactions between plants, soil, and microbial communities in relation to KVDS. This suggests that the syndrome is multifactorial and likely linked to an imbalance in soil microbial communities at the rhizosphere level, which can negatively affect soil health.
Effect of TetR Family Transcriptional Regulator PccD on Phytosterol Metabolism of Mycolicibacterium
Authors: Xiao Peiyao , Pan Delong , Li Fuyi , Liu Yuying , Huang Yang , Zhou Xiuling , Zhang Yang
Published: November 2024

Androstenedione (AD) is an important intermediate for the production of steroidal drugs. The process of transforming phytosterols into AD by Mycolicibacterium is mainly the degradation process of the phytosterol side chain, and the excessive accumulation of propionyl-CoA produced by Mycobacterium will produce toxic effects, which seriously restricts the transformation performance of strains. In this study, Mycolicibacterium sp. LZ2 (Msp) was used as the research object to study the transcription factor PccD of the TetR family, which has the role of propionyl-CoA metabolism regulation. By constructing overexpression and deletion strains of pccD, it was confirmed that pccD had an inhibitory effect on the transcription of propionyl-CoA carboxylase genes (pccA and pccB). Electrophoretic Mobility Shift Assay (EMSA) and DNase I footprint analysis demonstrated that PccD is directly involved in the transcriptional regulation of pccA and pccB and is a negative transcriptional regulator of the pcc operon. In the study of phytosterol transformation, the growth rate and bacterial viability of Msp-ΔpccD were higher than Msp, but the growth of Msp-pccD was inhibited. As a result of testing of intracellular propionyl-CoA levels and AD production yields, it was found that lower propionyl-CoA levels and higher AD production yields were observed in Msp-ΔpccD. The results expand the cognition of propionyl-CoA metabolism regulation and provide a theoretical basis and reference for the rational transformation of phytosterol transformation strains and secondary metabolite synthesis strains with propionyl-CoA as a substrate, which has important research significance.
Occurrence of Aggregatibacter actinomycetemcomitans and Its JP2 Genotype in a Cohort of 220 Western Australians with Unstable Periodontitis
Authors: Khzam Nabil , Kujan Omar , Haubek Dorte , Miranda Leticia Algarves
Published: November 2024

Aim: The main purpose of the present study was to investigate the carrier rate of Aggregatibacter actinomycetemcomitans and its JP2 genotype in a cohort of 200 Western Australians diagnosed with periodontitis. Materials and Methods: In this descriptive cross-sectional study, 220 consecutive patients with periodontitis, aged 18 years and older, were recruited to a specialist periodontal practice in Perth City. Every patient included in this study contributed three different intra-oral samples. Periodontal, radiographical, and microbiological assessments were performed. The samples were analysed using a polymerase chain reaction for the detection of Aggregatibacter actinomycetemcomitans and its JP2 genotype using the primers and conditions described previously. A Chi-square test and logistic regression analysis were performed to evaluate the results. Results: The prevalence of Aggregatibacter actinomycetemcomitans was 28.18%. The carrier rates of A. actinomycetemcomitans in the unstimulated saliva, cheek swabs, and pooled subgingival plaque samples were 21.80%, 19.50%, and 17.70%, respectively. There was a significant correlation between the severe form of periodontitis (stage IV, grade C) and younger age (p = 0.004), positive family history of periodontitis (p < 0.001), oral hygiene method (p < 0.001), and irregular dental visit attendance (p < 0.001). The binary logistic regression analysis revealed that having severe periodontitis risk increased almost three times in those who were young (OR: 2.812) and came from a family with a history of periodontal disease (OR: 3.194). However, the risk of severe periodontitis was five times higher in those patients with tooth loss due to periodontal disease (OR: 5.071). The highly leukotoxic JP2 genotype of Aggregatibacter actinomycetemcomitans was not detected. Conclusions: This study of a Western Australian cohort confirmed the low presence of Aggregatibacter actinomycetemcomitans and the complete absence of its JP2 genotype. Young age, family history of periodontal disease, lack of flossing, irregular dental visits, and tooth loss due to periodontitis were identified as potential risk factors for periodontitis stage IV, grade C in this cohort.
Assembly Characteristics and Influencing Factors of the Soil Microbial Community in the Typical Forest of Funiu Mountain
Authors: He Kunrun , Lai Yiran , Hu Shurui , Song Meiyi , Su Ye , Li Chunyang , Wu Xinle , Zhang Chunyue , Hua Yuanhang , Huang Jinyong , Guo Shujuan , Xu Yadong
Published: November 2024

Assessing the relationship between litter characteristics and soil microbial community traits across different forest types can enhance our understanding of the synergistic interactions among litter, soil, and microorganisms. This study focused on three representative forest types in the Funiu Mountains—Larix gmelinii (LG), Quercus aliena var. acutiserrata (QA), and Quercus aliena var. acutiserrata + Pinus armandii (QAPA). The findings indicated no significant differences in Chao1 among the three forests; however, the Shannon index exhibited an initial increase followed by a decline. NMDS and ANOSIM analyses revealed significant structural differences across these forest types. Network topological metrics (nodes, edges, average degree, and average path distance) for bacterial taxa were higher in LG and QA compared with QAPA. Additionally, LG and QA demonstrated significantly greater average niche breadth than QAPA. The results from the null models (the proportion occupied by dispersal limitation is 62.2%, 82.2%, and 64.4% in LG, QA, and QAPA), modified stochasticity ratio (LG: 0.708, QA: 0.664, and QAPA: 0.801), and neutral community models (LG: R2 = 0.665, QA: R2 = 0.630, and QAPA: R2 = 0.665) suggested that stochastic processes predominantly govern the assembly of soil bacterial communities. Random forest analysis alongside Mantel tests highlighted LTP (litter total phosphorus), STN (soil total nitrogen), MCP (carbon-to-phosphorus ratio of microbial biomass), and SCN (soil carbon-to-nitrogen ratio) as critical factors affecting bacterial niche width; conversely LCN (litter carbon-to-nitrogen ratio), RCP (ratio of dissolved carbon to phosphorus), MCP, and SCN emerged as key determinants influencing community assembly processes. Furthermore, the PLS-SEM results underscored how both litter characteristics along with soil properties—and their associated alpha diversity—impact variations in niche breadth while also shaping community assembly dynamics overall. This research provides vital insights into understanding synergistic relationships between litter quality, soil characteristics, and microbial community across diverse forest ecosystems.
Nature of the Pits on the Lattice-Matched InAlAs Layer Surface Grown on the (001) InP Substrate
Authors: Gulyaev Dmitrii V. , Abramkin Demid S. , Dmitriev Dmitriy V. , Toropov Alexander I. , Kolosovsky Eugeniy A. , Ponomarev Sergey A. , Kurus Nina N. , Milekhin Ilya A. , Zhuravlev Konstantin S.
Published: November 2024

The structural properties of lattice-matched InAlAs/InP layers grown by molecular beam epitaxy have been studied using atomic force microscopy, scanning electron microscopy and micro-photoluminescence spectroscopy. The formation of the surface pits with lateral sizes in the micron range and a depth of about 2 ÷ 10 nm has been detected. The InP substrate annealing temperature and value of InAlAs alloy composition deviation from the lattice-matched InxAl1−xAs/InP case (x = 0.52) control the density of pits ranging from 5 × 105 cm−2 ÷ 108 cm−2. The pit sizes are controlled by the InAlAs layer thickness and growth temperature. The correlation between the surface pits and threading dislocations has been detected. Moreover, the InAlAs surface is characterized by composition inhomogeneity with a magnitude of 0.7% with the cluster lateral sizes and density close to these parameters for surface pits. The experimental data allow us to suggest a model where the formation of surface pits and composition clusters is caused by the influence of a local strain field in the threading dislocation core vicinity on In adatoms incorporating kinetic.
Molecular Analysis of Aggregatibacter actinomycetemcomitans ApiA, a Multi-Functional Protein
Authors: Jacob Sera , Gusmao Luciana , Godboley Dipti , Velusamy Senthil Kumar , George Nisha , Schreiner Helen , Cugini Carla , Fine Daniel H.
Published: November 2024

Aggregatibacter actinomycetemcomitans ApiA is a trimeric autotransporter outer membrane protein (Omp) that participates in multiple functions, enabling A. actinomycetemcomitans to adapt to a variety of environments. The goal of this study is to identify regions in the apiA gene responsible for three of these functions: auto-aggregation, buccal epithelial cell binding, and complement resistance. Initially, apiA was expressed in Escherichia coli. Finally, wild-type A. actinomycetemcomitans and an apiA-deleted version were tested for their expression in the presence and absence of serum and genes related to stress adaptation, such as oxygen regulation, catalase activity, and Omp proteins. Sequential deletions in specific regions in the apiA gene as expressed in E. coli were examined for membrane proteins, which were confirmed by microscopy. The functional activity of epithelial cell binding, auto-aggregation, and complement resistance were then assessed, and regions in the apiA gene responsible for these functions were identified. A region spanning amino acids 186–217, when deleted, abrogated complement resistance and Factor H (FH) binding, while a region spanning amino acids 28–33 was related to epithelial cell binding. A 13-amino-acid peptide responsible for FH binding was shown to promote serum resistance. An apiA deletion in a clinical isolate (IDH781) was created and tested in the presence and/or absence of active and inactive serum and genes deemed responsible for prominent functional activity related to A. actinomycetemcomitans survival using qRT-PCR. These experiments suggested that apiA expression in IDH781 is involved in global regulatory mechanisms that are serum-dependent and show complement resistance. This is the first study to identify specific apiA regions in A. actinomycetemcomitans responsible for FH binding, complement resistance, and other stress-related functions. Moreover, the role of apiA in overall gene regulation was observed.
Photon Number States via Iterated Photon Addition in a Loop
Authors: Mendei Barna , Koniorczyk Mátyás , Homa Gábor , Adam Peter
Published: November 2024

We consider the probabilistic generation of time-bin photon number states from a train of single-photon pulses. We propose a simple interferometric feedback loop setup having a beam splitter and a possibly non-ideal detector. This Hong–Ou–Mandel-type scheme implements iterated photon additions. Our detailed study shows that up to four photons this simple setup can provide reasonable success probabilities and fidelities.
Effects of Thickness and Grain Size on Harmonic Generation in Thin AlN Films
Authors: Seres J. , Seres E. , Céspedes E. , Martinez-de-Olcoz L. , Zabala M. , Schumm T.
Published: November 2024

High-harmonic generation from solid films is an attractive method for converting infrared laser pulses to ultraviolet and vacuum ultraviolet wavelengths and for examining the films using the generation process. In this work, AlN thin films grown on a sapphire substrate are studied. Below-band-gap third harmonics and above-band-gap fifth harmonics were generated using a Ti:sapphire oscillator running at 800 nm. A strong enhancement of the fifth-harmonic signal in the forward direction was observed from thicker 39 nm and 100 nm films compared to thinner 8 nm and 17 nm films. For the fifth harmonic generated in the backward direction, and also for the third harmonic in both the forward and backward directions, only a weak dependence of the harmonic signal on the film thickness was measured. Using both X-ray diffraction and dependence of the fifth harmonic on the laser polarization measurements, these behaviors are attributed to the crystallization and the grain size of the films, promising fifth-harmonic generation as a suitable tool to study AlN film properties.
Blepharostoma vietnamicum (Marchantiophyta): A New Taxon from Indochina, the Unique Largest Species in the Genus
Authors: Bakalin Vadim A. , Vilnet Anna A. , Nguyen Van Sinh , Choi Seung Se
Published: November 2024

Blepharostoma is one of the most ancient extant liverwort genera, within which the genetic diversity is quite high, whereas the morphological diversity, owing to the supposed stasis, is quite low. Unusually large plants of this genus were collected in North Vietnam and are described here as new-to-science species via an integrative approach. The two studied specimens do not reveal variability in the sequenced ITS1-2 nrDNA and trnL-F cpDNA loci, are clearly separated from other species by the level of genetic distances, and maintain a stable position on the reconstructed phylogenetic trees. This species is characterized, in addition to the large overall size of the plants, by larger leaf segment cells and a mixed character of oil bodies (i.e., small homogeneous and larger finely papillose ones within one cell). A description of the new taxon; its diagnostic characteristics; photographs; and discussions regarding its ecology, morphological similarities, and potential distribution are provided.
Impact of Cassava Cultivars on Stylet Penetration Behavior and Settling of Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae)
Authors: Pimkornburee Sudarat , Pombud Supawadee , Buensanteai Kumri , Namanusart Weravart , Aiamla-or Sukanya , Roddee Jariya
Published: November 2024

This study investigates the settling preferences and feeding behavior of the Bemisia tabaci whitefly on six cassava cultivars using electrical penetration graph techniques. Six distinct electrical penetration graph waveforms—non-probing, stylet pathway, phloem salivation, phloem ingestion, intracellular puncture, and xylem feeding—were identified and analyzed. Significant differences in the frequency and duration of these waveforms were observed among the cassava cultivars. The whiteflies spent the majority of their time in the non-probing phase, particularly on the Huaybong 80, Kasetsart 50, Rayong 9, and Rayong 72 cultivars. CMR-89 cultivar exhibited higher total probe durations in the phloem salivation and ingestion waveforms, suggesting a greater potential for transmission of the Sri Lankan cassava mosaic virus. The study also examined trichome density and size across the cassava cultivars, revealing that CMR-89 had the highest density and small trichomes, while Huaybong 80 had the lowest density. Trichome characteristics significantly impacted whitefly behavior: larger trichomes were negatively correlated with whitefly settling, whereas higher trichome density was positively correlated with longer settling durations. These findings indicate that trichome-based resistance mechanisms are crucial in whitefly deterrence. Overall, the results suggest that cultivars with lower trichome density and larger trichomes are more resistant to whitefly infestation and subsequent Sri Lankan cassava mosaic virus transmission. These insights are valuable for cassava breeding programs focused on enhancing pest resistance, highlighting the importance of trichome characteristics in developing more resilient cassava varieties.
Integrating Morpho-Physiological, Biochemical, and Molecular Genotyping for Selection of Drought-Tolerant Pigeon Pea (Cajanus cajan L.) Genotypes at Seedling Stage
Authors: Ouma Benjamin O. , Mburu Kenneth , Kirui Geoffrey K. , Muge Edward K. , Nyaboga Evans N.
Published: November 2024

Pigeon pea (Cajanus cajan (L.) Millsp.), a potential legume as an economic source of protein, is commonly cultivated in tropical and subtropical regions of the world. It possesses medicinal properties and acts as a cash crop, benefiting low-income farmers economically. The identification of pigeon peas exhibiting drought tolerance has become crucial in addressing water scarcity issues in the agriculture sector. In addition, exploring the genetic diversity among genotypes is important for conservation, management of genetic resources, and breeding programs. The aim of this study was to evaluate the morpho-physiological and biochemical responses of selected pigeon pea genotypes under pot-induced water stress conditions through different field capacities as well as the genetic diversity using start codon targeted (SCoT) markers. A significant variation was observed for the physiological traits studied. The accumulation of fresh weight (FW) and dry weight (DW) was significantly reduced in moderate and severe drought stress conditions. The lowest % DW decrease was found in LM (35.39%), KAT (39.43%), and SM (46.98%) than other genotypes at severe drought stress. Analyses of physiological responses including the photosynthetic efficiency (Phi2), the chlorophyll content (SPAD), and the relative water content (RWC) revealed positive and negative correlations with various parameters, reflecting the impact of drought stress on the chlorophyll content. The results revealed that biochemical traits including the total phenolic content, soluble sugars, proline, total protein, total amino acids, and free amino acids were variably and significantly increased under water stress. Antioxidant enzyme activity levels, specifically ascorbate peroxidase (APX) and catalase, varied among the genotypes and in response to severe water stress, offering further insights into adaptive responses. The eight genotypes analysed by use of 20 SCoT markers revealed 206 alleles and an average of 10.3 alleles per locus. Genetic similarity ranged from 0.336 to 0.676, clustering the pigeon pea genotypes into two major groups by the unweighted pair group method of arithmetic averages (UPGMA) cluster analysis. Principal coordinate analysis (PCoA) explained 43.11% of genetic variation and based on analysis of molecular variance, a high genetic variation (80%) within populations was observed, emphasizing the potential for genetic improvement. Among the eight genotypes studied, LM and KAT were drought tolerant and genetically diverse and therefore could be used as parents for developing drought tolerance in pigeon pea.
Ethnopharmacological Study of Garrya laurifolia and Its Antidiabetic Effect in Rats
Authors: Estévez-Carmona María Mirian , Pablo-Pérez Saudy Saret , Almanza-Cruz Jesús Eduardo , Meléndez-Camargo María Estela , Arrieta-Baez Daniel , Cristóbal-Luna José Melesio , Franco-Colín Margarita
Published: November 2024

This study aimed to scientifically validate the traditional use of Garrya laurifolia (Gl) leaves as an antidiabetic agent attributed to a community in Mexico. The descriptive ethnobotanical study was conducted in San Miguel Tecpan, a municipality of Jilotzingo, State of Mexico, Mexico, where a structured questionnaire was applied to 44 inhabitants. In vivo studies evaluated the acute oral toxicity of Gl leaves in murine and the effects of a leaf infusion on glycemia in normoglycemic and diabetic rats; in addition, the interaction between Gl and metformin (Met) was also evaluated. The in vitro antioxidant activity of Gl was determined. The phytochemical screening and quantification of phenolic and flavonoid content of Gl leaves were performed. Gl had a high relative frequency of citation (0.68) among respondents. Gl had a low acute toxicity risk with LD50 > 5000 mg/kg. The extract had no hypoglycemic effect in normoglycemic rats, but it did have hypoglycemic and antihyperglycemic effects (250 and 500 mg/kg) in diabetic rats. The interaction between Gl (500 mg/kg) + Met (300 mg/kg) resulted in antidiabetic synergism. Gl showed strong antioxidant activity (93.1 ± 0.4%). Phytochemical screening revealed the presence of alkaloids, flavonoids, and some other phenolic compounds. The total phenol content was 77.9 ± 0.6 mg EQ/g and 87.7 ± 0.7 mg EAG/g, and the flavonoids content was 5.32 ± 0.2 mg EQ/g. UHPLC-MS/MS analysis identified chlorogenic acid, rutin, aucubin, luteolin 7-O-neohesperoside, and myricitrin. The findings support the potential use of Gl as a safe and effective antidiabetic agent.
A Review of Polyurethane Foams for Multi-Functional and High-Performance Applications
Authors: Dong Huanhuan , Li Shujing , Jia Zhixin , Luo Yuanfang , Chen Yongjun , Jiang Jiang , Ji Sheng
Published: November 2024

Polyurethane (PU) foams are cellular polymeric materials that have attracted much attention across various industries because of their versatile properties and potential for multifunctional applications. PU foams are involved in many innovations, especially in multi-functional and high-performance applications. Special attention is given to developing tailored PU foams for specific application needs. These foams have various applications including flame retardancy, sound absorption, radar absorption, EMI shielding, shape memory, and biomedical applications. The increasing demand for materials that can perform multiple functions while maintaining or enhancing their core properties has made PU foams a focal point of interest for engineers and researchers. This paper examines the challenges faced by the PU foam industry, particularly in developing multifunctional products, as well as the strategies for improving sustainability, such as producing PU foams from renewable resources and recycling existing materials.
Integration of Thermal Solar Power in an Existing Combined Cycle for a Reduction in Carbon Emissions and the Maximization of Cycle Efficiency
Authors: Abdelhalim Adham Mohamed , Meana-Fernández Andrés , Suarez-Ramon Ines
Published: November 2024

The energy transition towards renewable energy sources is vital for handling climate change, air pollution, and health-related problems. However, fossil fuels are still used worldwide as the main source for electricity generation. This work aims to contribute to the energy transition by exploring the best options for integrating a solar field within a combined cycle power plant. Different integration positions at the gas and steam cycles for the solar field were studied and compared under several operating conditions using a thermodynamic model implemented in MATLAB R2024a. Fuel-saving and power-boosting (flowrate and parameter boosting) strategies were studied. The results revealed that, for a maximum fuel savings of 7.97%, the best option was to integrate the field into the steam cycle before the economizer stage. With an integrated solar thermal power of 3 MW, carbon dioxide emissions from fuel combustion were reduced to 8.3 g/kWh. On the other hand, to maximize power plant generation, the best option was to integrate the field before the superheater, increasing power generation by 24.2% for a solar thermal power of 4 MW. To conclude, guidelines to select the best integration option depending on the desired outcome are provided.
Automated Gas Influx Handling Model and Mechanisms During Deep High-Temperature and High-Pressure Well Drilling
Authors: Zang Yanbin , Zhang Wenping , Xu Zhengming , Lu Jiayi , Deng Zhilu
Published: November 2024

The exploration and development of oil and gas resources in deep formations is a key strategic priority for national energy production. However, manual methods for handling gas kicks suffer from low operating accuracy and inefficiency during high-temperature and high-pressure deep well drilling. To address the need for real-time bottomhole pressure prediction and control, an efficient gas–liquid–solid computing model was developed based on the gas slip model and cuttings settling velocity model. By integrating this model with an automatic choke adjustment system, an automatic gas kick attenuation model for deep well drilling was established. Results show that, compared to the driller’s and wait-and-weight methods, the automatic gas kick attenuation method significantly reduces peak choke pressure due to its larger frictional pressure drop and higher cuttings hydrostatic pressure. The automatic attenuation method not only leads to an average reduction of 28.42% in maximum choke/casing pressure but also accelerates gas removal, achieving gas kick attenuation ten times faster than the driller’s method and seven times faster than the wait-and-weight method. The study also investigates the influence of gas solubility, well depth, gas influx volume, formation permeability, and drilling fluid volumetric flow rate on gas kick attenuation characteristics. The findings provide a solid foundation for improving the efficiency of gas kick management in deep well drilling operations.
Process Synthesis, Design and Techno-Economic Assessment of Malonic Acid Production
Authors: Ioannidou Virginia , Misailidis Nikiforos , Petrides Demetri , Georgiadis Michael C.
Published: November 2024

This work focuses on the design and techno-economic evaluation of an industrial facility for the production of malonic acid. The raw material utilized is commercial glucose syrup with a concentration of 95%. Based on a patent of Lygos, Inc., an innovative biotechnology research company, this study presents a comprehensive synthesis, design, and simulation framework for the production of malonic acid through oligosaccharide fermentation. An integrated process flowsheet is proposed and simulated using SuperPro Designer™. The analysis indicates that for an installation capacity of about 8000 MT/yr of the final product with a purity of 99.5%, the production cost is estimated at USD 7.92/kg. A comprehensive study of the capacity’s impact on economics reveals that this cost could decrease to as low as USD 6.05/kg. A parametric analysis and optimization conducted at the flowsheet level identifies opportunities for further reducing production costs, laying the groundwork for a potential decrease in the product’s selling price.
Side-by-Side Economic Process Model for the Comparison and Evaluation of Magnetic Bead-Based Processes and Legacy Process for the Manufacturing of Monoclonal Antibodies
Authors: Brechmann Nils A. , Stamatis Christos , Farid Suzanne S. , Chotteau Veronique , Eriksson Kristofer
Published: November 2024

This study models two alternative downstream processes based on magnetic separation with the objective of understanding the economic feasibility of these processes compared to the traditional mAb process. The key focus lies in the economic understanding of the cell harvest and capture steps in the models. Here, the models revealed that integrating cell removal and product capture in a single operation is the main factor driving the unified productivity between USP and the magnetic bead-based processes. This results in significant economic benefits, such as savings in both the cost of goods per gram of mAb and fixed costs, as well as increasing annual facility output. The predicted savings potential approaches 38% for COGs, 17% for capital investment, and 40% for annual facility output. For mammalian cell-based manufacturing, the magnetic separation-based DSP provides a highly valuable option due to its integration of several individual unit operations compared to the traditional process both in reducing process time and cost and accommodating higher demands.
Image Dehazing Technique Based on DenseNet and the Denoising Self-Encoder
Authors: Liu Kunxiang , Yang Yue , Tian Yan , Mao Haixia
Published: November 2024

The application value of low-quality photos taken in foggy conditions is significantly lower than that of clear images. As a result, restoring the original image information and enhancing the quality of damaged images on cloudy days are crucial. Commonly used deep learning techniques like DehazeNet, AOD-Net, and Li have shown encouraging progress in the study of image dehazing applications. However, these methods suffer from a shallow network structure leading to limited network estimation capability, reliance on atmospheric scattering models to generate the final results that are prone to error accumulation, as well as unstable training and slow convergence. Aiming at these problems, this paper proposes an improved end-to-end convolutional neural network method based on the denoising self-encoder-DenseNet (DAE-DenseNet), where the denoising self-encoder is used as the main body of the network structure, the encoder extracts the features of haze images, the decoder performs the feature reconstruction to recover the image, and the boosting module further performs the feature fusion locally and globally, and finally outputs the dehazed image. Testing the defogging effect in the public dataset, the PSNR index of DAE-DenseNet is 22.60, which is much higher than other methods. Experiments have proved that the dehazing method designed in this paper is better than other algorithms to a certain extent, and there is no color oversaturation or an excessive dehazing phenomenon in the image after dehazing. The dehazing results are the closest to the real image and the viewing experience feels natural and comfortable, with the image dehazing effect being very competitive.
Fault Diagnosis of Aircraft Hydraulic Pipeline Clamps Based on Improved KPCA and WOA–KELM
Authors: Liu Chunli , Zhang Xiaolong , Bai Jiarui
Published: November 2024

Due to the complexity and diversity of aviation hydraulic pipeline systems, there has been a lack of qualitative formulas or characteristic indicators to describe clamp failures within these systems. In this paper, based on the data-driven idea, an improved KPCA-based feature extraction method is proposed and combined with the optimized KELM for fault diagnosis and condition monitoring of aviation hydraulic line clamps. Firstly, the kernel parameters of KPCA are combined using polynomial and Gaussian kernels based on their proportional weights. Secondly, a GA–PSO (Genetic Algorithm–Particle Swarm Optimization) hybrid algorithm is employed to optimize the kernel parameters, selecting 13 time-domain and 4 frequency-domain feature indicators to form the initial feature dataset, which is then subjected to dimensionality reduction using the improved KPCA. Finally, diagnosis is conducted using a KELM optimized by the whale optimization algorithm. The results indicate that, across multiple diagnostic trials, the average diagnostic accuracy can reach 99.99%, providing a feasible approach for the precise diagnosis of clamp faults in aviation hydraulic pipeline systems.
Metal-Exchanged Phosphomolybdic Acid Salts-Catalyzed Esterification of Levulinic Acid
Authors: Silva Márcio José da , Rodrigues Alana Alves , Taba Wilton Keisuke
Published: November 2024

We examined the effectiveness of metal-exchanged phosphomolybdic acid salts in converting levulinic acid, derived from biomass, into valuable products (alkyl levulinate). We prepared salts of phosphomolybdic acid using different metals (Fe3+, Al3+, Zn2+, Cu2+, Mn2+, Ni2+, and Co2+). The influence of metal cations on the conversion and selectivity of the reactions was assessed. We found that the salts prepared with iron and aluminum phosphomolybdate were the most effective catalysts for the esterification of levulinic acid with methanol, with the conversion and selectivity tending towards 100% after 6 h of reaction at a temperature of 323 K. The effect of catalyst loading and its recovery and reuse was evaluated; the results from the reaction using aluminum phosphomolybdate remained similar for four cycles of use. The influence of temperature on conversion and selectivity was investigated between 298 and 353 K. The reactivity of different alcohols with a carbon chain size of C1-C4 was assessed and conversions above 65% were obtained for all alcohols tested under the conditions evaluated, except for tert-butyl alcohol. These catalysts are a promising alternative to the traditional soluble and corrosive Brønsted acid catalysts. The superior performance of these catalysts was ascribed to the higher pH decline triggered by the hydrolysis of these metal cations.
Achieving High Performance with Less Energy Consumption: Intermittent Ultrasonic-Mediated Operation Mode for Fe/V Non-Aqueous Redox Flow Battery
Authors: Long Hui , Sun Peizhuo , Zhu Haochen , Ma Qiang , Shen Xiaozhong , Su Huaneng , Flox Cristina , Xu Qian
Published: November 2024

Deep eutectic solvents (DESs) have attracted much attention as sustainable electrolytes for redox flow batteries. Despite the tremendous advantages of DES-based electrolytes, their high viscosity property has a negative effect on their mass transfer, limiting current density and power density. The ultrasonic effect has been demonstrated as an efficient strategy to improve mass transfer characteristics. Incorporating ultrasonic waves into a deep eutectic solvent (DES) electrolyte enhances the mobility of redox-active ions, thereby accelerating the reaction dynamics of the Fe(III)/Fe(II) redox pair. This enhancement makes it suitable for use in non-aqueous electrolyte-based redox flow batteries. However, it is necessary to consider the loss of ultrasonic on the internal structure of the battery, as well as the loss of battery component materials and ultrasonic energy consumption in practical applications. Moreover, the continuous extension of the duration of ultrasonic action not only hardly leads to a more significant improvement of the battery performance, but is also detrimental to the energy and economic savings. Herein, intermittent ultrasound is used to overcome the quality transfer problem and reduce the operating cost. Good electrochemical performance enhancement is maintained with a roughly 50% reduction in energy consumption values. The mechanism as well as the visualization of the pulsed ultrasonic field on each half cell has been envisaged through fundamental characterization. Finally, the feasibility of interrupted ultrasonic activation applied to Fe/V RFB using DES electrolytes has been demonstrated, demonstrating similar behavior with continuous ultrasonic operation. Therefore, the interrupted ultrasonic field has been found to be a more effective operation mode in terms of energy cost, avoiding alternative undesirable effects like overheating or corrosion of materials.
Correction: Correia et al. Sustainability Assessment of 2G Bioethanol Production from Residual Lignocellulosic Biomass. Processes 2024, 12, 987
Authors: Correia Bárbara , Matos Henrique A. , Lopes Tiago F. , Marques Susana , Gírio Francisco
Published: November 2024

In the original publication [...]
Sensorless Position Control in High-Speed Domain of PMSM Based on Improved Adaptive Sliding Mode Observer
Authors: Shi Liangtong , Lv Minghao , Li Pengwei
Published: November 2024

To improve the speed buffering and position tracking accuracy of medium–high-speed permanent magnet synchronous motor (PMSM), a sensorless control method based on an improved sliding mode observer is proposed. By the mathematical model of the built-in PMSM, an improved adaptive super-twisting sliding mode observer is constructed. Based on the LSTA-SMO with a linear term of observation error, a sliding mode coefficient can be adjusted in real time according to the change in rotational speed. In view of the high harmonic content of the output back electromotive force, the adaptive adjustment strategy for the back electromotive force is adopted. In addition, in order to improve the estimation accuracy and resistance ability of the observer, the rotor position error was taken as the disturbance term, and the third-order extended state observer (ESO) was constructed to estimate the rotational speed and rotor position through the motor mechanical motion equation. The proposed method is validated in Matlab and compared with the conventional linear super twisted observer. The simulation results show that the proposed method enables the observer to operate stably in a wide velocity domain and reduces the velocity estimation error to 6.7 rpm and the position estimation accuracy error to 0.0005 rad at high speeds, which improves the anti-interference capability.
Ex Ante Construction of Flow Pattern Maps for Pulsating Heat Pipes
Authors: Alqahtani Ali Ahmed , Bertola Volfango
Published: November 2024

A novel methodology is proposed for the development of empirical flow pattern maps for pulsating heat pipes (PHPs), which relies on the concept of virtual superficial velocity of the liquid and vapour phases. The virtual superficial velocity of each phase is defined using solely the design and operational parameters of the pulsating heat pipe, allowing the resulting flow pattern map to serve as a predictive instrument. This contrasts with existing flow pattern maps that necessitate direct measurements of temperatures and/or velocities within one or more channels of the pulsating heat pipe. Specifically, the virtual superficial velocities are derived from the relative significance of the driving forces and the resistances encountered by each phase during flow. The proposed methodology is validated using flow visualisation datasets obtained from two separate experimental campaigns conducted on flat-plate polypropylene pulsating heat pipe prototypes featuring transparent walls and meandering channels with three turns, five turns, seven turns, and eleven turns, respectively. The PHP prototypes were tested for gravity levels ranging between 0 g and 1 g and heat inputs ranging from 5 W to 35 W. The proposed approach enables the identification of empirical boundaries for flow pattern transitions as well as the establishment of an empirical criterion for start-up.
The Identification, Separation, and Clamp Function of an Intelligent Flexible Blueberry Picking Robot
Authors: Liu Xiaohong , Li Peifu , Hu Bo , Yin Hao , Wang Zexian , Li Wenxin , Xu Yanxia , Li Baogang
Published: November 2024

Identifying fruit maturity accurately and achieving damage-free harvesting are challenges in designing blueberry-picking robots. This paper presents an intelligent flexible picking system. First, we trained a deep learning-based YOLOv8n network to locate the position of the fruit and determine fruit ripeness. We used a neural network to establish the relationship between fruit hardness and shape parameters, achieving an adaptive gripping force for different fruits. To address the issue of dense clusters in some blueberry varieties, we designed a fruit separation subsystem using a combination of flow field analysis and pressure-sensitive experiments. The results show that the mean average precision can reach 84.62%, the precision is 94.49%, the recall is 83.85%, the F1 score is 88.85%, and the test time is 0.12 s, which can meet the requirements for blueberry fruit recognition accuracy and speed. The spacing between closely packed fruits can increase by 4 mm, and the damage-free picking rate exceeds 92%, achieving stable, damage-free harvesting.
The Influence of Mg, Na, and Li Oxides on the CO2 Sorption Properties of Natural Zeolite
Authors: Mambetova Manshuk , Dossumov Kusman , Yergaziyeva Gaukhar
Published: November 2024

This study presents a comparative analysis of the CO2 sorption properties of natural zeolites sourced from the Tayzhuzgen (Tg) and Shankanay (Sh) deposits in Kazakhstan. The Tayzhuzgen zeolite was characterized by a Si/Al ratio of 5.6, suggesting partial dealumination, and demonstrated enhanced specific surface area following mechanical activation. Modification of the Tayzhuzgen zeolite with magnesium oxide significantly improved its CO2 sorption capacity, reaching 8.46 mmol CO2/g, attributed to the formation of the CaMg(Si2O6) phase and the resulting increase in basic active sites. TPD-CO2 analysis confirmed that MgO/Tg exhibited the highest basicity of the modified samples, further validating its potential for CO2 capture applications.
Comparative Evaluation of Wound Healing Efficacy of Bombyx mori L. Body Extracts, Gland Extracts, and Cocoon for the Treatment of Second-Degree Burns: A Pilot Study
Authors: Kyriaki Alexandra , Vidali Margarita , Vitsos Andreas , Harizanis Paschalis , Sfiniadakis Ioannis , Barda Christina , Simirioti Eleftheria , Terezaki Asimina , Rallis Michail Christou
Published: November 2024

Background: The silkworm (Bombyx mori L.) and its cocoon are rich in bioactive proteins like sericin and fibroin, as well as enzymes such as serrapeptase, which possess anti-inflammatory and skin-healing properties. This study aimed to evaluate the in vivo effects of various silkworm products, including cocoon patches and extracts from the silkworm body and glands, on the healing of second-degree burns. Methods: Hairless, female SKH-2 mice were used to model second-degree burns. The study tested formulations containing 1%, 10%, or 20% silkworm body or gland extracts, as well as cocoon-derived patches. In addition to histopathological and clinical assessments, the study measured parameters including burn size, hydration, transepidermal water loss and thickness. Results: The results of this study demonstrated that, in terms of primary outcomes (complete healing), both the silkworm cocoon and the 20% body extract significantly promoted wound healing, with similar efficacy. All body extracts showed statistical significance in wound area reduction, while the gland extracts had no significant effect. Histopathological evaluation confirmed the superior healing potential of the body extracts increasing by increased concentration and cocoon. This novel insight into the therapeutic properties of silkworm body extracts opens new opportunities for the development of cost-effective, renewable second-degree burn healing treatments.
Characteristics and Paleoenvironment of the Niutitang Shale Reservoir in the Zhenba Area
Authors: Tian Tao , Chang Wei , Zhang Pei , Yang Jiahui , Zhang Li , Wang Tianzi
Published: November 2024

The lack of in-depth analysis on the reservoir characteristics and the paleoenvironmental conditions of the Niutitang Formation in the study area has led to an unclear understanding of its geological background. In this study, core samples from well SZY1 were selected, and X-ray diffraction (XRD), scanning electron microscopy (SEM), and quantitative elemental analysis were employed to systematically investigate the reservoir properties and paleoenvironment of the shales. The results indicate that the Niutitang Formation shales form a low-porosity, low-permeability reservoir. By utilizing indicators such as the chemical index of alteration (CIA) and elemental ratios, the study delves into the paleoclimate and paleoproductivity of the region. The (La/Yb)n ratio is approximately 1, indicating a rapid deposition rate that is beneficial for the accumulation and preservation of organic matter. The chondrite-normalized and North American Shale Composite (NASC)-normalized rare earth element (REE) distribution patterns of the shales show consistent trends with minimal variation, reflecting the presence of mixed sources for the sediments in the study area. Analysis reveals that the Niutitang Formation shales are enriched in light rare-earth elements (LREEs) with a negative europium anomaly, and the primary source rocks are sedimentary and granitic, located far from areas of seafloor hydrothermal activity. The NiEF and CuEF values suggest high paleoproductivity, and the shales were deposited in an anoxic-reducing environment. The depositional environments of the Marcellus and Utica shales in the United States, the Wufeng-Longmaxi black shales in the Changning area of the Sichuan Basin, and the shales in the study area are similar, characterized by anoxic reducing conditions and well-developed fractures. The thermal evolution degree of the study area is relatively moderate, currently in the peak gas generation stage, with the reservoir quality rated as medium to high, indicating good potential for hydrocarbon accumulation and promising exploration prospects.
Impact of Aging and Recycling on Optical Properties of Cardboard for Circular Economy
Authors: Mirković Ivana Bolanča , Bolanča Zdenka , Medek Goran
Published: November 2024

This study investigates the effects of aging and recycling on the optical properties of paperboard, which is key to advancing circular economy practices in packaging. Methods included deinking flotation of cardboard made from sea algae and eco-conventional cardboard of unexposed and exposed samples in a xenon test chamber. Optical measurements were performed on the obtained laboratory paper sheets. Measurements for the chromatic coefficients ΔL*, Δa* and Δb*, as well as the CIE whiteness from comparison of the fluorescent component in the cardboard, were carried out under two light sources, D65 and UV. Regression analysis was used to quantify the statistical significance of these changes over time, i.e., in the aging process. The results revealed significant effects of both aging and recycling on the chromatic coefficients, with ΔL* and Δa* decreasing, while Δb* initially increased before decreasing. The influence of the fluorescent component is reduced by recycling the samples. Opacity measurements showed an initial increase in values that decreased with the aging of the samples, which indicates structural changes in the material. This research contributes to the circular economy by providing insight into the durability and optical properties of recycled cardboard, helping to develop sustainable packaging solutions.