bluuebunny/crossref_metadata_2025 huggingface dataset:
Sustainable Hydrogen Production from Starch Aqueous Suspensions over a Cd0.7Zn0.3S-Based Photocatalyst
Authors: Kurenkova Anna Y. , Medvedeva Tatiana B. , Gromov Nikolay V. , Bukhtiyarov Andrey V. , Gerasimov Evgeny Y. , Cherepanova Svetlana V. , Kozlova Ekaterina A.
Published: July 2021

We explored the photoreforming of rice and corn starch with simultaneous hydrogen production over a Cd0.7Zn0.3S-based photocatalyst under visible light irradiation. The photocatalyst was characterized by UV–vis diffuse reflectance spectroscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. The influence of starch pretreatment conditions, such as hydrolysis temperature and alkaline concentration, on the reaction rate was studied. The maximum rate of H2 evolution was 730 μmol·h−1·g−1, with AQE = 1.8% at 450 nm, in the solution obtained after starch hydrolysis in 5 M NaOH at 70 °C. The composition of the aqueous phase of the suspension before and after the photocatalytic reaction was studied via high-performance liquid chromatography, and such products as glucose and sodium gluconate, acetate, formate, glycolate, and lactate were found after the photocatalytic reaction.
A Bio-Based Alginate Aerogel as an Ionic Liquid Support for the Efficient Synthesis of Cyclic Carbonates from CO2 and Epoxides
Authors: Paninho Ana B. , Mustapa Ana N. , Mahmudov Kamran T. , Pombeiro Armando J. L. , Guedes da Silva M. Fátima C. , Bermejo María D. , Martín Ángel , Cocero María J. , Nunes Ana V. M.
Published: July 2021

In this work, the ionic liquid [Aliquat][Cl] was supported into alginate and silica aerogel matrices and applied as a catalyst in the cycloaddition reaction between CO2 and a bio-based epoxide (limonene oxide). The efficiency of the alginate aerogel system is much higher than that of the silica one. The method of wet impregnation was used for the impregnation of the aerogel with [Aliquat][Cl] and a zinc complex. The procedure originated a well-defined thin solvent film on the surface of support materials. Final materials were characterised by Fourier Transform Infrared Spectroscopy, N2 Adsorption–Desorption Analysis, X-ray diffraction, atomic absorption and Field Emission Scanning Microscopy. Several catalytic tests were performed in a high-pressure apparatus at 353.2 K and 4 MPa of CO2.
Synthesis of Alpha Olefins: Catalytic Decarbonylation of Carboxylic Acids and Vegetable Oil Deodorizer Distillate (VODD)
Authors: Lee Hang-Wai , Yung Ka-Fu
Published: July 2021

Decarbonylation of carboxylic acids provides an effective protocol for producing alpha olefins; however, previous literature has focused on the palladium-bisphosphine catalysts and has only sporadically studied the palladium-monophosphine catalyst. To investigate the catalytic activity of the palladium-monophosphine catalyst on decarbonylation of carboxylic acids, new monophosphine ligands were synthesized (NP-1, NP-2, CP-1 and CP-2). By employing (1–3 mol%) palladium-naphthylphosphine catalysts, various carboxylic acids were converted into corresponding alpha alkenes with good yields and selectivity within a short period of time. Vegetable oil deodorizer distillate (VODD), which is a by-product from the vegetable oil refinery process, was found to be rich in free fatty acids and there is great interest in turning vegetable oil deodorizer distillate into value-added compounds. It is noteworthy that our catalytic system could be applied to convert vegetable oil deodorizer distillate (VODD) into diesel-like hydrocarbons in a good yield.
Are We Ready for Migrastatics?
Authors: Solomon Jonathan , Raškova Magdalena , Rösel Daniel , Brábek Jan , Gil-Henn Hava
Published: July 2021

Metastasis accounts for the highest mortality rates in solid tumor cancer patients. However, research and development have neglected this most lethal characteristic and, instead, have concentrated on the hallmarks of cancer that make tumor cells highly proliferative and distinctive from nonmalignant cells. The concentration on invasion and metastasis can be one of the most meaningful advancements in cancer investigation. Importantly, metastasis-free survival (MFS) was recently approved by the Food and Drug Administration (FDA) as a novel primary endpoint in clinical trials and has been used to evaluate the prognosis of patients with nonmetastatic castration-resistant prostate cancer and soft tissue sarcoma. This new definition enables to shift the focus of research and development in cancer therapeutics toward metastasis and to change the emphasis from using tumor shrinkage as a benchmark for indicating the efficacy of treatment to using MFS as a more representative endpoint for antimetastatic drugs. This perspective outlines the possibility to use this novel endpoint in other solid cancers, and examples of large clinical trials are given in which MFS is defined as an endpoint and/or in which antimetastatic strategies are being examined. These advances now open the door for the rapid development of antimetastatic therapies, which could be used in combination with standard cytotoxic cancer therapies. With pioneer research on metastasis prevention on the rise and the underlying biomechanisms of tumor cell motility and invasion explored further than ever before, we believe an intensified focus on antimetastatic properties will shape this era of cancer translational research.
Translating Treg Therapy for Inflammatory Bowel Disease in Humanized Mice
Authors: Negi Sushmita , Saini Sheetal , Tandel Nikunj , Sahu Kiran , Mishra Ravi P.N. , Tyagi Rajeev K.
Published: July 2021

Crohn’s disease and ulcerative colitis, two major forms of inflammatory bowel disease (IBD) in humans, afflicted in genetically predisposed individuals due to dysregulated immune response directed against constituents of gut flora. The defective immune responses mounted against the regulatory mechanisms amplify and maintain the IBD-induced mucosal inflammation. Therefore, restoring the balance between inflammatory and anti-inflammatory immunepathways in the gut may contribute to halting the IBD-associated tissue-damaging immune response. Phenotypic and functional characterization of various immune-suppressive T cells (regulatory T cells; Tregs) over the last decade has been used to optimize the procedures for in vitro expansion of these cells for developing therapeutic interventional strategies. In this paper, we review the mechanisms of action and functional importance of Tregs during the pathogenesis of IBD and modulating the disease induced inflammation as well as role of mouse models including humanized mice repopulated with the human immune system (HIS) to study the IBD. “Humanized” mouse models provide new tools to analyze human Treg ontogeny, immunobiology, and therapy and the role of Tregs in developing interventional strategies against IBD. Overall, humanized mouse models replicate the human conditions and prove a viable tool to study molecular functions of human Tregs to harness their therapeutic potential.
Inflammation and Aging of Hematopoietic Stem Cells in Their Niche
Authors: Yang Daozheng , de Haan Gerald
Published: July 2021

Hematopoietic stem cells (HSCs) sustain the lifelong production of all blood cell lineages. The functioning of aged HSCs is impaired, including a declined repopulation capacity and myeloid and platelet-restricted differentiation. Both cell-intrinsic and microenvironmental extrinsic factors contribute to HSC aging. Recent studies highlight the emerging role of inflammation in contributing to HSC aging. In this review, we summarize the recent finding of age-associated changes of HSCs and the bone marrow niche in which they lodge, and discuss how inflammation may drive HSC aging.
Activation of Nrf2 in Astrocytes Suppressed PD-Like Phenotypes via Antioxidant and Autophagy Pathways in Rat and Drosophila Models
Authors: Guo Qing , Wang Bing , Wang Xiaobo , Smith Wanli W. , Zhu Yi , Liu Zhaohui
Published: July 2021

The oxidative-stress-induced impairment of autophagy plays a critical role in the pathogenesis of Parkinson’s disease (PD). In this study, we investigated whether the alteration of Nrf2 in astrocytes protected against 6-OHDA (6-hydroxydopamine)- and rotenone-induced PD-like phenotypes, using 6-OHDA-induced rat PD and rotenone-induced Drosophila PD models. In the PD rat model, we found that Nrf2 expression was significantly higher in astrocytes than in neurons. CDDO-Me (CDDO methyl ester, an Nrf2 inducer) administration attenuated PD-like neurodegeneration mainly through Nrf2 activation in astrocytes by activating the antioxidant signaling pathway and enhancing autophagy in the substantia nigra and striatum. In the PD Drosophila model, the overexpression of Nrf2 in glial cells displayed more protective effects than such overexpression in neurons. Increased Nrf2 expression in glial cells significantly reduced oxidative stress and enhanced autophagy in the brain tissue. The administration of the Nrf2 inhibitor ML385 reduced the neuroprotective effect of Nrf2 through the inhibition of the antioxidant signaling pathway and autophagy pathway. The autophagy inhibitor 3-MA partially reduced the neuroprotective effect of Nrf2 through the inhibition of the autophagy pathway, but not the antioxidant signaling pathway. Moreover, Nrf2 knockdown caused neurodegeneration in flies. Treatment with CDDO-Me attenuated the Nrf2-knockdown-induced degeneration in the flies through the activation of the antioxidant signaling pathway and increased autophagy. An autophagy inducer, rapamycin, partially rescued the neurodegeneration in Nrf2-knockdown Drosophila by enhancing autophagy. Our results indicate that the activation of the Nrf2-linked signaling pathways in glial cells plays an important neuroprotective role in PD models. Our findings not only provide a novel insight into the mechanisms of Nrf2–antioxidant–autophagy signaling, but also provide potential targets for PD interventions.
Interobserver Reliability of Pirani and Dimeglio Scores in the Clinical Evaluation of Idiopathic Congenital Clubfoot
Authors: Pavone Vito , Vescio Andrea , Culmone Annalisa , Caldaci Alessia , Rosa Piermario , Costarella Luciano , Testa Gianluca
Published: July 2021

Background: Dimeglio (DimS) and Pirani (PirS) scores are the most common scores used in congenital talipes equinovarus (CTEV) clinical practice. The aim of this study was to evaluate the interobserver reliability of these scores and how clinical practice can influence the clinical outcome of clubfoot through the DimS and Pirs. Methods: Fifty-four feet were assessed by six trained independent observers through the DimS and PirS: three consultants (OS), and three residents (RS) divided into three pediatric orthopaedic surgeons (PeO) and three non-pediatric orthopaedic surgeons (NPeO). Results: The PirS and DimS Scores were strongly correlated. In the same way, OS and RS, PirS, and DimS scores were strongly correlated, and the interobserver reliability ranked “good” in the comparison between PeO and NPeO. In fully trained paediatric orthopaedic surgeons, an “excellent” interobserver reliability was found but was only “good” in the NPeO cohort. Conclusions: In conclusion, after careful preparation, at least six months of observation of children with CTEV, PirS and DimS proved to be valid in terms of clinical evaluation. However, more experience with CTEV leads to a better clinical evaluation.
New Result on the Feedback Capacity of the Action-Dependent Dirty Paper Wiretap Channel
Authors: Xie Guangfen , Dai Bin
Published: July 2021

The Gaussian wiretap channel with noncausal state interference available at the transmitter, which is also called the dirty paper wiretap channel (DP-WTC), has been extensively studied in the literature. Recently, it has been shown that taking actions on the corrupted state interference of the DP-WTC (also called the action-dependent DP-WTC) helps to increase the secrecy capacity of the DP-WTC. Subsequently, it has been shown that channel feedback further increases the secrecy capacity of the action-dependent DP-WTC (AD-DP-WTC), and a sub-optimal feedback scheme is proposed for this feedback model. In this paper, a two-step hybrid scheme and a corresponding new lower bound on the secrecy capacity of the AD-DP-WTC with noiseless feedback are proposed. The proposed new lower bound is shown to be optimal (achieving the secrecy capacity) and tighter than the existing one in the literature for some cases, and the results of this paper are further explained via numerical examples.
On the Potential of Augmented Reality for Mathematics Teaching with the Application cleARmaths
Authors: Schutera Stefanie , Schnierle Marc , Wu Mathilde , Pertzel Tim , Seybold Jonathan , Bauer Patricia , Teutscher Dennis , Raedle Matthias , Heß-Mohr Natascha , Röck Sascha , Krause Mathias J.
Published: July 2021

Learning content in mathematics, such as vector geometry, is still predominantly taught in an abstract manner, as the visualization and interaction of three-dimensional problems are limited with classical forms of teaching such as blackboard lessons or exercise sheets. This research article proposes the use of augmented reality (AR) in mathematics education. The proposed approach aims at easing the learning process related to vector geometry currently taught in senior mathematics classes by using intuitive visualization. The article introduces the concept of AR and presents the didactic foundations and the influence on the learning process based on an extensive literature review. Although studies see great potential in the use of AR for teaching mathematics, the method has so far hardly been used in schools. This can be mainly explained by the technological entry barrier of AR and the lack of simple, robust AR applications, in particular for vector geometry. To fill this gap, the authors developed “cleARmaths”, a developed android application for augmented reality-based teaching in vector geometry that allows widespread use. As a didactical concept, some example exercises sessions with the app are proposed, demonstrating how the app could be used in a mathematics classroom. Finally, the app was evaluated in a mathematics class and the results analyzed in a detailed study. It was found by the teacher and students to be beneficial and amusing, demonstrating the potential for AR in mathematics classes.
Distributed Event-Triggered Secondary Recovery Control for Islanded Microgrids
Authors: Wan Xiaofeng , Tian Ye , Wu Jingwan , Ding Xiaohua , Tu Huipeng
Published: July 2021

Distributed cooperative control methods are widely used in the islanded microgrid control system. To solve the deviation of frequency and voltage caused by the droop control, it is necessary to recovery the frequency and voltage to the rated value using a secondary control strategy. However, the traditional communication method relies on the continuous periodic one, which makes the communication burden of the islanded microgrid system heavy and conflicts with the actual operation of the power grid. Using the secondary recovery control method based on the distributed event-triggered method, we conserve communication resources by reducing the number of transmissions of sampled data and achieving the recovery control of the frequency and voltage and the original proportional sharing of active power. In addition, we analyze the stability of the distributed event-triggered strategy and build a microgrid system with MATLAB/Simulink to verify the effectiveness of the control method. Furthermore, we compare with a traditional periodic communication system and demonstrate the superiority of our distributed event-triggered approach.
Optimization-Based Antenna Miniaturization Using Adaptively Adjusted Penalty Factors
Authors: Mahrokh Marzieh , Koziel Slawomir
Published: July 2021

The continuing trend for miniaturization of electronic devices necessitates size reduction of the comprising components and circuitry. Specifically, integrated circuit-antenna modules therein require compact radiators in applications such as 5G communications, implantable and on-body devices, or internet of things (IoT). The conflict between the demands for compact size and electrical and field performance can be mitigated by means of constrained numerical optimization. Evaluation of performance-related constraints requires expensive electromagnetic (EM) analysis of the system at hand; therefore, their explicit handling is inconvenient. A workaround is the penalty function approach where the primary objective (typically, antenna size) is complemented by additional terms quantifying possible constraint violations. The penalty coefficients that determine contributions of these terms are normally adjusted manually, which hinders precise control over antenna performance figures and often leads to inferior results in terms of achieved miniaturization rates. This paper proposes a novel algorithm featuring an automated adjustment of the penalty factors throughout the optimization process. Our methodology is validated using three broadband antenna structures. The obtained results demonstrate that the presented adaptive adjustment permits a precise control over the constraint violations while leading to better miniaturization rates as compared to manual penalty term setup.
Effects of Crop–Hedgerow Intercropping on the Soil Physicochemical Properties and Crop Yield on Sloping Cultivated Lands in a Purple Soil of Southwestern China
Authors: Lei Ping , Ni Chengsheng , Chen Fangxin , Wang Sheng , Zhong Shouqin , Tan Shaojun , Ni Jiupai , Xie Deti
Published: July 2021

Crop–hedgerow intercropping systems are important agroforestry systems for preventing soil degradation and soil nutrient losses on sloping cultivated land in the Three Gorges Reservoir (TGR) area of China. However, the mechanism by which hedgerow spatial layouts and the planting patterns affect soil nutrients and crop yields is still uncertain. A two-year field experiment was performed on a 10° slope to investigate the effects of slope position and different crop–hedgerow intercropping systems on soil physicochemical properties and crop yields. The treatments were a two-belt mulberry contour hedgerow (TM), a two-belt compound mulberry–vetiver hedgerow (TCMV), a two-belt compound mulberry–alfalfa hedgerow (TCMA), a seven-year-old two-belt mulberry contour hedgerow (7YTM), a seven-year-old mulberry border hedgerow (7YBM), a seven-year-old pure mulberry (7YPM), and a control treatment (CT, no hedgerows). In all treatments, except 7YPM, there was a significant (p < 0.05) increase in crop yield, clay content, soil total nitrogen (STN), acid-hydrolyzable nitrogen (AHN), and soil organic carbon (SOC) with declining slope position, whereas soil bulk density (BD), sand content, and soil pH showed the opposite trend. In TM, TCMV, TCMA, and 7YTM, the mustard yields and soil properties were better than those in CT, and there was no significant (p > 0.05) difference in mustard yield or soil properties between the upper-middle and lower-middle slope positions. Compared with CT, TCMV, and TCMA increased mustard yields by 8.28% and 9.86%, respectively, while 7YTM, 7YBM, and 7YPM reduced mustard yields by 7.69%, 17.69%, and 29.73%, respectively. TCMV and TCMA were confirmed to be viable intercropping systems for significantly reducing nutrient losses, improving soil quality, and changing soil nutrient distributions to maintain optimum crop yields on sloping lands.
A Stand-Level Comparison of Carbon and Nitrogen Distribution in an Exotic Japanese Cedar Plantation and a Natural Oak Stand
Authors: Baek Gyeongwon , Bae Eun-Ji , Kim Choonsig
Published: July 2021

This study compared carbon (C) and nitrogen (N) distribution at a stand level in an exotic Japanese cedar (Cryptomeria japonica D. Don) plantation and a natural Serrata oak (Quercus serrata Murray) stand growing under similar site conditions in South Korea. The aboveground biomass (stems, branches, and leaves) of 20 trees (10 of each species), the forest floor, and the mineral soils to a depth of 30 cm were sampled to determine C and N concentrations. Except in branches, C concentrations were significantly higher (p < 0.05) in the Japanese cedar plantation than in the Serrata oak stand, whereas N concentrations, except in the stem bark, were significantly lower in the Japanese cedar plantation. Reforestation with an exotic coniferous species significantly increased the C stocks in the aboveground biomass and the N stocks in the forest floor and mineral soils compared with a natural oak stand. The N stocks in the aboveground biomass were dependent on either the N concentrations or the C stocks in the tree components, whereas soil C and N stocks were negatively related to soil fertility parameters such as C/N ratio. Although it is uncertain which factors are responsible for the difference in aboveground C and soil N stocks following the establishment of Japanese cedar plantations on former natural Serrata oak stands, tree replacement may have an impact on C and N allocation within different forest compartments.
Development and Validation of a Multi-Locus PCR-HRM Method for Species Identification in Mytilus Genus with Food Authenticity Purposes
Authors: Quintrel Marianela , Jilberto Felipe , Sepúlveda Matías , Marín María Elisa , Véliz David , Araneda Cristián , Larraín María Angélica
Published: July 2021

DNA-based methods using informative markers such as single nucleotide polymorphism (SNPs) are suitable for reliable species identification (SI) needed to enforce compliance with seafood labelling regulations (EU No.1379/2013). We developed a panel of 10 highly informative SNPs to be genotyped by PCR-High resolution melting (HRM) for SI in the Mytilus genus through in silico and in vitro stages. Its fitness for purpose and concordance were assessed by an internal validation process and by the transference to a second laboratory. The method was applicable to identify M. chilensis, M. edulis, M. galloprovincialis and M. trossulus mussels, fresh, frozen and canned with brine, oil and scallop sauce, but not in preserves containing acetic acid (wine vinegar) and tomato sauce. False-positive and negative rates were zero. Sensitivity, expressed as limit of detection (LOD), ranged between 5 and 8 ng/μL. The method was robust against small variations in DNA quality, annealing time and temperature, primer concentration, reaction volume and HRM kit. Reference materials and 220 samples were tested in an inter-laboratory assay obtaining an “almost perfect agreement” (κ = 0.925, p < 0.001). In conclusion, the method was suitable for the intended use and to be applied in the seafood industry.
Effects of Different Processing Methods and Internal Components on Physicochemical Properties and Glycemic Index of Adzuki Bean Powder
Authors: Ren Feiyue , Yang Xiaoxue , Wang Lili , Zhou Sumei
Published: July 2021

The estimated glycemic index (eGI) value of adzuki bean powder prepared by steamed cooking (SC), extruded cooking (EC) and roller cooking (RC) was studied comparatively. Results showed that RC had the highest eGI, with 80.1, and both EC and SC resulted in a lower eGI value of 70.0 and 49.7, respectively. Compared with the EC and RC methods, the SC method provided a more intact physical barrier for starch digestion, resulting in a less destroyed cell structure. As the essential components that form the cell wall, the study further investigated the effects of protein and fiber on physicochemical properties, in vitro starch digestibility and the eGI of adzuki bean powder processed with the SC method. Viscozyme and Protamax were used to obtain the deprotein and defiber samples. Results showed that the SC treatment with Viscozyme and Protamax, respectively, had significant effects on in vitro starch digestibility. The eGI of different samples were given as follows: steamed cooking adzuki bean powder (49.7) < deproteined adzuki bean powder (60.5) < defibered adzuki bean powder (83.1), which indicates that fiber may have a greater influence on the eGI than protein.
Embryo Screening for Polygenic Disease Risk: Recent Advances and Ethical Considerations
Authors: Tellier Laurent C. A. M. , Eccles Jennifer , Treff Nathan R. , Lello Louis , Fishel Simon , Hsu Stephen
Published: July 2021

Machine learning methods applied to large genomic datasets (such as those used in GWAS) have led to the creation of polygenic risk scores (PRSs) that can be used identify individuals who are at highly elevated risk for important disease conditions, such as coronary artery disease (CAD), diabetes, hypertension, breast cancer, and many more. PRSs have been validated in large population groups across multiple continents and are under evaluation for widespread clinical use in adult health. It has been shown that PRSs can be used to identify which of two individuals is at a lower disease risk, even when these two individuals are siblings from a shared family environment. The relative risk reduction (RRR) from choosing an embryo with a lower PRS (with respect to one chosen at random) can be quantified by using these sibling results. New technology for precise embryo genotyping allows more sophisticated preimplantation ranking with better results than the current method of selection that is based on morphology. We review the advances described above and discuss related ethical considerations.
Understanding the Dynamics of a Coastal Lagoon: Drivers, Exchanges, State of the Environment, Consequences and Responses
Authors: Chacón Abarca Samantha , Chávez Valeria , Silva Rodolfo , Martínez M. Luisa , Anfuso Giorgio
Published: July 2021

At present, many coastal ecosystems worldwide are highly affected by anthropic activities. La Mancha lagoon, in the state of Veracruz, Mexico, is an important ecosystem due to the wide array of ecosystem services that it provides. In this paper, an analysis of the environmental balances of the lagoon is outlined, using the Drivers, Exchanges, State of the Environment, Consequences and Responses (DESCR) tool. The methodological framework considers the interrelationships between the natural systems and the forces of change that alter the performance of the natural environment, in order to provide an overview of actions that may reduce negative consequences. The study area has been impacted by anthropic development, such as changes in land use for agricultural and livestock activities, loss of mangroves due to logging and modifications, carried out by local fishermen, to the natural hydrodynamics of the lagoon that alter the salinity and affect the ecosystem dynamics. Following analysis of the area, using the DESCR tool, the responses proposed include long-term environmental impact evaluation, with the aim of preserving the local coastal ecosystems.
Postural Adjustments and Kinematic Index Finger Features in Frail Older Adults under Different Equilibrium Constraints
Authors: Callegari Bianca , Kubicki Alexandre , Saunier Ghislain , Duarte Manuela Brito , Almeida Gizele Cristina da Silva , Alves Bruno Mazziotti Oliveira , Amorim César Ferreira , Garcez Daniela Rosa , Souza Givago da Silva , Mourey France
Published: July 2021

Background: Anticipatory postural adjustments (APAs) are significantly affected by age and may represent restrictions on functional independence. Previous studies in young adults have already highlighted that changing postural stability (i.e., seated vs. upright posture) affects the motor planning and APAs. In frail older adults (FOAs), the effect of these different conditions of postural stability have not yet been established, and the present study aimed to disentangle this issue. Methods: Participants executed an arm-pointing task to reach a diode immediately after it turned on, under different conditions of stability (seated with and without foot support and in an upright posture). A kinematic profile of the index finger and postural electromyographic data were registered in their dominant-side leg muscles: tibialis anterior, soleus, rectus femoris, and semitendinosus. Results: The main finding of this study was that the adopted posture and body stabilization in FOAs did not reflect differences in APAs or kinematic features. In addition, they did not present an optimal APA, since postural muscles are recruited simultaneously with the deltoid. Conclusion: Thus, FOAs seem to use a single non-optimal motor plan to assist with task performance and counterbalance perturbation forces in which they present similar APAs and do not modify their kinematics features under different equilibrium constraints.
Genome-Wide Analysis and Functional Characterization of the UDP-Glycosyltransferase Family in Grapes
Authors: Wei Yongzan , Mu Huayuan , Xu Guangzhao , Wang Yi , Li Yang , Li Shaohua , Wang Lijun
Published: July 2021

Grape (Vitis vinifera) produces a variety of secondary metabolites, which can enhance nutrients and flavor in fruit and wine. Uridine diphosphate-glycosyltransferases (UGTs) are primarily responsible for the availability of secondary metabolites by glycosylation modification. Here, a total of 228 putative UGTs were identified in V. vinifera, and VvUGTs were clustered into 15 groups (A to O) and unevenly distributed on 18 chromosomes. Diverse VvUGT members from 12 groups were transcribed, and they responded to different external stresses. More than 72% of VvUGT members were expressed at one or more stages of grape fruit development, and the expression levels of 34 VvUGT members increased gradually with fruit ripening. The VvUGT members of different groups may be involved in the synthesis and accumulation of flavonoid glycosides, glycosidically bound volatiles, and stilbenes. These results will provide guidance for further research on the functions and regulating mechanisms of UGT genes.
Evaluation of Groundwater Using an Integrated Approach of Entropy Weight and Stochastic Simulation: A Case Study in East Region of Beijing
Authors: Zhang Yongxiang , Jia Ruitao , Wu Jin , Wang Huaqing , Luo Zhuoran
Published: July 2021

Groundwater is an important source of water in Beijing. Hydrochemical composition and water quality are the key factors to determine the availability of groundwater. Therefore, an improved integrated weight water quality index approach (IWQI) combining the entropy weight method and the stochastic simulation method is proposed. Through systematic investigation of groundwater chemical composition in different periods, using a hydrogeochemical diagram, multivariate statistics and spatial interpolation analysis, the spatial evolution characteristics and genetic mechanism of groundwater chemistry are discussed. The results show that the groundwater in the study area is weakly alkaline and low mineralized water. The south part of the study area showed higher concentrations of total dissolved solids, total hardness and NO3−-N in the dry season and wet season, and the main hydrochemical types are HCO3−-Ca and HCO3−-Ca-Mg. The natural source mechanism of the groundwater chemical components in Chaoyang District includes rock weathering, dissolution and cation exchange, while the human-made sources are mainly residents and industrial activities. Improved IWQI evaluation results indicate that water quality decreases from southwest to northeast along groundwater flow path. The water quality index (WQI) method cannot reflect the trend of groundwater. Sensitivity analysis indicated that the improved IWQI method could describe the overall water quality reliably, accurately and stably.
Persistent Misperceptions about Nicotine among US Physicians: Results from a Randomized Survey Experiment
Authors: Bover Manderski Michelle T. , Steinberg Michael B. , Wackowski Olivia A. , Singh Binu , Young William J. , Delnevo Cristine D.
Published: July 2021

We conducted a survey experiment among US physicians to evaluate whether question wording impacted perceptions about the health effects of nicotine. 926 physicians were randomized to receive one of two versions of a question matrix that asked about the “extent to which they agree or disagree that ‘nicotine’ (Version 1) or ‘nicotine, on its own,’ (Version 2) directly contributes to” birth defects, cardiovascular disease (CVD), cancer, depression, and chronic obstructive pulmonary disease (COPD). We evaluated whether question condition predicted strong agreement and/or agreement with each statement, and assessed demographic correlates of each outcome while adjusting for question version. Physicians who received Version 2 were less likely to “strongly agree” that nicotine directly caused birth defects (Prevalence Ratio (PR) 0.84, 95% CI 0.72–0.98), CVD (PR 0.89, 95% CI 0.84–0.95), cancer (PR 0.81, 95% CI 0.75–0.87), and COPD (PR 0.78, 95% CI 0.72–0.84). Females were more likely to “strongly agree” that nicotine directly contributes to birth defects and cancer, and family physicians were most likely to “strongly agree” that nicotine directly contributes to CVD, cancer, and COPD. Question wording is important when measuring physicians’ beliefs about nicotine; however, even after accounting for question version, misperceptions about the direct health effects of nicotine were common and varied by sex and specialty.
Omega-3 Index and Clinical Outcomes of Severe COVID-19: Preliminary Results of a Cross-Sectional Study
Authors: Zapata B. Rodrigo , Müller José Miguel , Vásquez Juan Enrique , Ravera Franco , Lago Gustavo , Cañón Eduardo , Castañeda Daniella , Pradenas Madelaine , Ramírez-Santana Muriel
Published: July 2021

The potentially detrimental effects of the worldwide deficiency of Omega-3 fatty acids on the COVID-19 pandemic have been underestimated. The Omega-3 Index (O3I), clinical variables, biometric indices, and nutritional information were directly determined for 74 patients with severe COVID-19 and 10 healthy quality-control subjects. The relationships between the OI3 and mechanical ventilation (MV) and death were analyzed. Results: Patients with COVID-19 exhibited low O3I (mean: 4.15%; range: 3.06–6.14%)—consistent with insufficient fish and Omega-3 supplement consumption, and markedly lower than the healthy control subjects (mean: 7.84%; range: 4.65–10.71%). Inverse associations were observed between O3I and MV (OR = 0.459; C.I.: 0.211–0.997) and death (OR = 0.28; C.I.: 0.08–0.985) in severe COVID-19, even after adjusting for sex, age, and well-known risk factors. Conclusion: We present preliminary evidence to support the hypothesis that the risk of severe COVID-19 can be stratified by the O3I quartile. Further investigations are needed to assess the value of the O3I as a blood marker for COVID-19.
A Bibliometric Analysis on Nonpoint Source Pollution: Current Status, Development, and Future
Authors: Lei Ping , Shrestha Ram Kumar , Zhu Bing , Han Suju , Yang Hongbin , Tan Shaojun , Ni Jiupai , Xie Deti
Published: July 2021

Nonpoint source pollution (NPS) has become the leading factor of global water quality problems, attracting great attention from governments and researchers in various countries. Based on this situation, understanding the current research status of NPS can help guide future research. However, most of the current reviews only describe the research status of some specific aspects but fail to quantify the research hotspots and development trends on the whole, which limits the overall understanding of NPS. In this paper, bibliometrics was used to study the current status, hotspots, and frontiers of NPS research during 1991–2015, and the future research development was predicted. Over the past 15 years, there has been a remarkable growth trend in publication output, and the participation of countries/territories has also increased. Journal of Environmental Quality, Journal of Hydrology, and Total Environmental Science were the top three journals. Sharpley AN and Arnold JG from the USA were the most productive authors with the best quality articles. The major author clusters and research regions are located in North America and Europe, followed by East Asia. The United States dominates this research field, with the largest number of independent and collaborative articles. Chinese authors gained more attention through international cooperation. Keyword analysis confirmed that water quality and nutrients were the main concerns of NPS pollution research, which mainly involved a number of research topics, such as pollutant emission reduction research and the evaluation and simulation of pollutants’ migration and their transformation under different situations, while pesticides were less of a concern, which suggests that the abuse of pesticides has come under control. Meanwhile, SWAT was the dominating model in the last decade partly because it satisfied the growing needs of watershed-scale management.
Effects of e-Health Training and Social Support Interventions for Informal Caregivers of People with Dementia—A Narrative Review
Authors: Sitges-Maciá Esther , Bonete-López Beatriz , Sánchez-Cabaco Antonio , Oltra-Cucarella Javier
Published: July 2021

Along with the burden commonly experienced by informal caregivers (ICs) of people with dementia (PwD), associated with the progressive decline that accompanies dementia, the lockdown due to the public health crisis has had a great negative impact on the emotional wellbeing, physical health, and social relationships of ICs. Support interventions through telemedicine represent an opportunity for ICs to learn the skills required for the care and maintenance of social networks. In this work, a narrative review of the effects of e-health training and social support interventions was carried out. A literature search was conducted using the ProQuest, Ovid, and Scopus databases. Information regarding social support (SS), psychological interventions, and training for the management of medications and behavioral changes was extracted. One hundred and nine studies were included in this review. Forums and training platforms were the main tools for ICs. The most effective platforms to improve SS include the participation of both ICs and health professionals. However, no significant improvements in objective caring skills were identified. Platforms developed specifically for ICs should be based in tools that ICs are familiar with, because many ICs have not yet incorporated Information and Communication Technologies in many activities of their daily lives. Education in the digitalization to ICs of PwD should be one of the priority objectives in telehealth interventions.
Modelling Exposure by Spraying Activities—Status and Future Needs
Authors: Hahn Stefan , Meyer Jessica , Roitzsch Michael , Delmaar Christiaan , Koch Wolfgang , Schwarz Janine , Heiland Astrid , Schendel Thomas , Jung Christian , Schlüter Urs
Published: July 2021

Spray applications enable a uniform distribution of substances on surfaces in a highly efficient manner, and thus can be found at workplaces as well as in consumer environments. A systematic literature review on modelling exposure by spraying activities has been conducted and status and further needs have been discussed with experts at a symposium. This review summarizes the current knowledge about models and their level of conservatism and accuracy. We found that extraction of relevant information on model performance for spraying from published studies and interpretation of model accuracy proved to be challenging, as the studies often accounted for only a small part of potential spray applications. To achieve a better quality of exposure estimates in the future, more systematic evaluation of models is beneficial, taking into account a representative variety of spray equipment and application patterns. Model predictions could be improved by more accurate consideration of variation in spray equipment. Inter-model harmonization with regard to spray input parameters and appropriate grouping of spray exposure situations is recommended. From a user perspective, a platform or database with information on different spraying equipment and techniques and agreed standard parameters for specific spraying scenarios from different regulations may be useful.
Physicians’ Knowledge, Attitude and Practice of Generic Substitution in China: A Cross-Sectional Online Survey
Authors: Zhao Mingyue , Zhang Lingyi , Feng Zhitong , Fang Yu
Published: July 2021

The purpose of this study is to investigate physicians’ knowledge, attitudes and practice of generic medicine substitutions in China. We conducted a cross-sectional online questionnaire survey on physicians from secondary or tertiary hospitals in China from 2020 December to 2021 April. Descriptive statistical and ordered logistic regression were used for analysis. A total of 1225 physicians were included in the final analysis, and only 330 (26.94%) of them scored 4 or above in the knowledge part, which means that the physicians have a good knowledge of generic substitutions. Of the total, 586 (47.83%) agreed or strongly agreed that generic drugs could be substituted for originator drugs and 585 (47.75%) always or often prescribed generic medicines. The percentage of physicians with a positive attitude toward or that practice prescribing generic medicine is below 50%, which needs to be improved in China. Physicians’ knowledge, their attitude toward generic substitution, if familiar with the policy of generic substitution, and incentives for prescribing generic medicines are influencing factors for the practice of generic substitution. Our studies show that the practice of generic substitution by physicians could be improved by several measures in China. We suggested that the physicians should be taught more about the bulk-buy policy and the generic-originator equivalence evaluation policy. Moreover, government incentives to promote generic substitution should be established. Our study also suggested that physicians with less working experience and female physicians should learn more about generic substitution.
Development and Validation of Nurses’ Well-Being and Caring Nurse–Patient Interaction Model: A Psychometric Study
Authors: Chung Hui-Chun , Chen Yueh-Chih , Chang Shu-Chuan , Hsu Wen-Lin , Hsieh Tsung-Cheng
Published: July 2021

Nurses’ care nurse–patient interaction (CNPI) competence is critical for improving nursing care quality. The focus is the psychological quality of nurses, which may be derived from their sense of well-being. The purpose of this study was to develop a conceptual model of nurses’ well-being and their CNPI competence. A cross-sectional survey was conducted with a total of 212 valid questionnaires obtained from a medical center. Structural equation modeling analysis was performed to validate the conceptual model. The results demonstrated the positive correlation between two constructs of nurses’ well-being (“contentment” and “joyfulness”) and CNPI competence. A positive correlation between nurses’ CNPI competence and their health-promoting lifestyle and work environment satisfaction was supported. Among the constructs of CNPI competence, “respect patients’ life experience” was the most correlated with their well-being, health-promoting lifestyle, and work environment satisfaction. The constructs of these three scales, which showed a middle correlation with CNPI competence, are psychological constructs rather than material constructs. When nurses have a greater sense of well-being, a positive attitude towards life, and feel supported and respected in their work environment, their CNPI competence increases. The findings of this study provide important insights and can serve as empirical evidence for nursing managers to enhance nurses’ CNPI competence.
Correction: Maning et al. Antagonistic Roles of GRK2 and GRK5 in Cardiac Aldosterone Signaling Reveal GRK5-Mediated Cardioprotection via Mineralocorticoid Receptor Inhibition. Int. J. Mol. Sci. 2020, 21, 2868
Authors: Maning Jennifer , McCrink Katie , Pollard Celina , Desimine Victoria , Ghandour Jennifer , Perez Arianna , Cora Natalie , Ferraino Krysten , Parker Barbara , Brill Ava , Aukszi Beatrix , Lymperopoulos Anastasios
Published: July 2021

The authors wish to make the following correction to this paper [...]
The Sodium-Glucose Cotransporter-2 Inhibitor Canagliflozin Alleviates Endothelial Dysfunction Following In Vitro Vascular Ischemia/Reperfusion Injury in Rats
Authors: Korkmaz-Icöz Sevil , Kocer Cenk , Sayour Alex A. , Kraft Patricia , Benker Mona I. , Abulizi Sophia , Georgevici Adrian-Iustin , Brlecic Paige , Radovits Tamás , Loganathan Sivakkanan , Karck Matthias , Szabó Gábor
Published: July 2021

Vascular ischemia/reperfusion injury (IRI) contributes to graft failure and adverse clinical outcomes following coronary artery bypass grafting. Sodium-glucose-cotransporter (SGLT)-2-inhibitors have been shown to protect against myocardial IRI, irrespective of diabetes. We hypothesized that adding canagliflozin (CANA) (an SGLT-2-inhibitor) to saline protects vascular grafts from IRI. Aortic rings from non-diabetic rats were isolated and immediately mounted in organ bath chambers (control, n = 9–10 rats) or underwent cold ischemic preservation in saline, supplemented either with a DMSO vehicle (IR, n = 8–10 rats) or 50µM CANA (IR + CANA, n = 9–11 rats). Vascular function was measured, the expression of 88 genes using PCR-array was analyzed, and feature selection using machine learning was applied. Impaired maximal vasorelaxation to acetylcholine in the IR-group compared to controls was significantly ameliorated by CANA (IR 31.7 ± 3.2% vs. IR + CANA 51.9 ± 2.5%, p < 0.05). IR altered the expression of 17 genes. Ccl2, Ccl3, Ccl4, CxCr4, Fos, Icam1, Il10, Il1a and Il1b have been found to have the highest interaction. Compared to controls, IR significantly upregulated the mRNA expressions of Il1a and Il6, which were reduced by 1.5- and 1.75-fold with CANA, respectively. CANA significantly prevented the upregulation of Cd40, downregulated NoxO1 gene expression, decreased ICAM-1 and nitrotyrosine, and increased PECAM-1 immunoreactivity. CANA alleviates endothelial dysfunction following IRI.