bluuebunny/crossref_metadata_2025 huggingface dataset:
BIMBINGAN BELAJAR UNTUK MENINGKATKAN MOTIVASI BERPRESTASI ATLET BOLA VOLI O2SN KABUPATEN MAJALENGKA
Authors: Erdiyanti Yucky Putri , Febriyanto Bayu Dwi
Published: April 2020

Penelitian dilatarbelakangi oleh kurangnya motivasi berprestasi di bidang akademik atlet bola voli di kabupaten majalengka. Salah satu upaya dalam meningkatkan motivasi berprestasi dalam  bidang  akademik   yaitu  menggunakan  bimbingan  belajar.  Salah satu wahana untuk meningkatkan kualitas sumber daya manusia tersebut dapat dilakukan melalui bidang keolahragaan.  Permainan bolavoli merupakan salah satu cabang olahraga yang sudah sangat memasyarakat. Tujuan melakukan olahraga  bolavoli sangat  beragam,  dari  yang  sekedar  untuk  hiburan  atau  rekreasi,  olahraga kesehatan, olahraga pendidikan sampai dengan yang bertujuan untuk mencapai prestasi yang maksimal. Penelitian ini bertujuan untuk (1)Mengetahui profil motivasi belajar atlet bola voli sebelum dan sesudah diberikan bimbingan belajar, (2)Menghasilkan program bimbingan belajar untuk meningkatkan motivasi  belajar  atlet  bola voli, (3)Memperoleh  data empiric tentang efektivitas program bimbingan belajar untuk meningkatkan motivasi belajar atlet. Untuk menghasilkan bimbingan belajar yang efektif ditempuh dengan studi pendahuluan, pengembangan instrument, uji kelayakan  instrumen,  pengembangan  bimbingan  belajar,  uji  kelayakan  bimbingan  belajar dan uji coba bimbingan belajar Pendekatan penelitian ini  menggunakan pendekatan kuantitatif dengan metode  kuasi  eksperimen  dan  desainnya  adalah  pretest-postest  control  grup  desain terhadap atlet bola voli O2SN di kabupaten Majalengka. Pengumpulan data menggunakan alat ukur berupa instrumen motivasi berprestasi. Hasil penelitian menunjukkan secara empirik, perlakuan berupa bimbingan belajar teruji tidak efektif dalam meningkatkan motivasi berprestasi atlet bola voli. Diharapkan mengetahui tingkat motivasi berprestasi atlet bola voli diawal perkuliahan, agar dapat merencanakan program yang harus dilakukan Motivasi berprestasi.
PENGEMBANGAN LEMBAR KERJA PESERTA DIDIK (LKPD) MATEMATIKA BERBASIS MODEL REALISTIC MATHEMATICS EDUCATION
Authors: Septian Ruly , Irianto Sony , Andriani Ana
Published: April 2020

Proses pembelajaran akan berjalan efektif jika ketersediaan bahan ajar lengkap selesai. Lembar Kerja Peserta Didik (LKPD) atau Lembar Kerja Siswa adalah salah satu bahan ajar yang dapat digunakan dalam pembelajaran. Ini adalah penelitian dan pengembangan studi menggunakan model 4-D menurut Thiagarajan, Semmel, dan Semmel yang terdiri dari tiga tahap, yaitu: mendefinisikan, merancang, mengembangkan, dan menyebar. Ini bertujuan untuk menghasilkan LKPD menggunakan model pendidikan matematika realistis pada topik volume angka padat tidak teratur di kelas lima di sekolah dasar. Penelitian dilakukan di SD Negeri 3 Karangnanas, UPK (Unit Pendidikan Kecamatan) Sokaraja, Kabupaten Banyumas, dengan populasi 24 siswa kelas lima. Peneliti menggunakan teknik tes dan non-tes termasuk pretest, posttest, validasi ahli, penilaian guru, dan kuesioner respon siswa untuk pengumpulan data. Hasil penelitian ini menunjukkan bahwa: 1) pengembangan lembar kerja siswa matematika (LKPD) berdasarkan model pendidikan matematika realistik di kelas V di SD Negeri 3 Karangnanas dapat digunakan sebagai bahan ajar sebagai bahan ajar matematika pelengkap di sekolah dasar , 2) penilaian guru pada lembar kerja memperoleh skor 95%, dengan kriteria "Sangat Baik", 3) respons siswa terhadap lembar kerja matematika memperoleh skor rata-rata 93%, dengan kriteria "Sangat Setuju ", 4) lembar kerja mempengaruhi prestasi belajar siswa. Lembar kerja dapat difungsikan untuk melengkapi bahan ajar matematika, yang dapat digunakan dalam proses pembelajaran di sekolah dasar.
PENERAPAN PENDEKATAN SINERGIS BERBASIS KELOMPOK DALAM MENINGKATKAN HASIL BELAJAR SISWA DALAM PEMBELAJARAN MATEMATIKA
Authors: Sukari
Published: April 2020

Penelitian ini bertujuan untuk meningkatkan hasil belajar matematika dalam pembelajaran operasi hitung bilangan pecahan campuran pada siswa kelas IV SDN 2 marikangen, Cirebon.  Jenis penelitian ini adalah Penelitian Tindakan Kelas dengan subyek penelitian adalah siswa kelas IV SDN 2 marikangen, Cirebon. Data hasil penelitian diperoleh dari observasi dan tes hasil belajar. Data yang diperoleh dianalisis secara deskriptif kuantitatif dan deskriptif kualitatif.  Hasil penelitian menunjukkan bahwa rata-rata hasil belajar menggunakan pendekatan sinergis berbasis kelompok mengalami peningkatan. Dengan demikian dapat disimpulkan bahwa Penerapan Pendekatan Sinergis Berbasis Kelompok dapat meningkatkan hasil belajar siswa dalam pembelajaran matematika pada Materi Operasi Hitung Campuran di kelas IV SDN 2 marikangen
HUBUNGAN GAYA KEPEMIMPINAN GURU DENGAN PRESTASI BELAJAR SISWA SEKOLAH DASAR
Authors: Asikin
Published: April 2020

Penelitian ini bertujuan untuk mengetahui hubungan gaya kepemimpinan situasional guru dalam pembelajaran terhadap prestasi belajar siswa kelas V Sekolah Dasar Se Gugus Lima, Rajagaluh tahun ajaran 2019/2020. Hipotesis penelitian ini adalah ada hubungan positif antara gaya kepemimpinan situasional guru dalam pembelajaran terhadap prestasi siswa kelas V Sekolah Dasar Se-Gugus Lima, Rajagaluh tahun ajaran 2019/2020. Penelitian ini menggunakan pendekatan penelitian kuantitatif dengan teknik pengumpulan data menggunakan survei. Hasil penelitian menunjukkan bahwagaya kepemimpinan situasional guru berhubungan signifikan dengan prestasi siswa. Sehingga dapat ditarik kesimpulan bahwa gaya kepemimpinan situasional guru dalam pembelajaran ada hubungan positif terhadap prestasi belajar siswa kelas V Sekolah Dasar Se-Gugus Lima, Rajagaluh tahun ajaran 2019/2020.
Coping with Broken Achievement-Related Expectations in Students from Elementary School: An Experimental Study
Authors: Pinquart Martin , Block Helena
Published: April 2020

The experiment analyzed reactions of 115 first- to fourth-graders after realizing that they performed worse than they expected in a computer game. Based on the VIOLEX model of expectation violation, we assessed immunization against achievement feedback, assimilation (striving to increase performance), and accommodation (willingness for expectation change). Students decreased their expectations for the next round of the game after getting feedback indicating that they performed worse than expected, and younger students decreased their expectation more than older students did. After giving them some time for thinking about their achievement (and for finding potential excuses for the lower-than-expected performance), younger students slightly increased their expectation, while older students showed a further drop in expectation. Analyses of verbal responses showed that immunizing against the discrepant feedback was followed by an increase in expectations, whereas assimilative and accommodative tendencies did not predict persistence and change in expectations. Conclusions are drawn for future research.
THE EFFECT OF CADMIUM ON ALUMINUM COPPER MANGAN ALLOY
Authors: Nguyen Thong Duy , Nguyen Du Vinh
Published: April 2020

This paper presents the effect of Cadium in Aluminium alloys on mechanical property and phase transformation in the alloys. The Aluminium 4,8Cu 0,6Mn 0,2Ti alloys that contain between 0,150 and 0,288 Cadmium not only enhanced strength but also stabilized physical-mechanical properties. These characteristics are particularly shown on high temperature conditions. Although Cadmium does not participate in the resistance phases of the alloys, it still can maintain and dispose the vacancies in Al-Cu-Mn alloys after tempering. That's reason why Cadmium can control the precipitation of the resistance phase - CuAl2- in the tempering process.
Enthusiastic discussions on solid physic and material science at SPMS2019
Authors: Dao Van-Duong , Nguyen Duc Chien , Stręk Wiesław
Published: April 2020

At 11th National Conference of Solid Physics and Material Science (SPMS2019), to which eminent scientists (5 plenary speakers and 16 invited speakers) were invited, most of the discussion focused on solid physic and material science. Around 300 researchers interested in the subject attended the conference and actively participate in the discussion. There were more than 200 reports with five specialized subcommittees: (A) Physics and magnetic materials, (B) Semiconductor and dielectric physics, (C) Materials - semiconductor components - dielectric, (D) Biomedical materials - agriculture, energy - environment, (E) Composite materials - metals - ceramics. Besides, numerous reports submitted to the Journal of Science and Technology (Vietnam Academy of Science and Technology), full-text reports sent to the Organizing Committee, after a critical review process, had been summarized and published in the Collection of conference reports.
The optimal generator dispatching with uncertain conditions for grid-connected microgrid
Authors: Quoc Dung Phan , Thi Thanh Binh Phan , Dinh Minh Pham , Minh Hung Tran , Duc Hung Nguyen
Published: April 2020

Nowadays, the penetration of wind and solar sources is relatively high in Micro Grid. Wind speed and solar radiation forecasting hardly gives an exact value and leads to the values in intervals. Therefore forecasted output powers of these sources are also in the intervals. The constraint on power balance in Micro Grid has the right-hand-side uncertainty, in the interval. So for Micro-Grid in grid connection mode, the presence of the distributed generations based on wind and solar energy sources makes optimal dispatching problems of distributed generations become an uncertainty problem. The optimal solutions for the lower and upper ends of this interval are the best and the worst optimal solution. This paper proposes to treat the above problem as the optimal problem with two objectives: reach the best and the worst solution. The principle of fuzzy set and the Particle Swarm Optimization algorithm will be applied for solving the multi-objective problem. The final optimal value will belong to an interval. Meanwhile, the output power of the swing generator varies to respond to the uncertainty of wind and solar source power. An example of a low-voltage MG with three distributed generators is considered with two cases: connecting to the utility grid via the circuit breaker and via power controller.
粘土心墙坝填筑过程质量检测与控制途径
Authors: 卢 江
Published: April 2020

本文结合实际的水利工程案例,对粘土心墙坝填筑过程之中的质量检测和控制进行分析。文章首先介绍了本次所研究项目的工程概况,然后对本次工程之中的试验检测项目及其质量控制措施进行分析。
“河长制”在水环境治理中的效用探析
Authors: 崔 绍峰
Published: April 2020

可持续发展背景下,水环境作为人类生存环境中不可或缺的一部分,在社会长期发展中遭受到不同程度的破坏,加剧水资源浪费和污染,加强水环境治理和管理,对于人类社会长治久安具有深远意义。尤其是进入新世纪以来,我国的环境污染问题愈加严峻,在水环境治理中实行“河长制”,发挥新型工作机制优势,有助于改善水环境治理工作中的不足,全面提升工作水平,以便于实现长期水资源保护和利用,谋求人类社会可持续发展。本文就水环境治理中“河长制”的效用进行阐述分析,针对其中的不足提出有效性改善建议,以求为后续相关工作开展提供一定促进作用。
工业循环冷却水节水技术研究
Authors: 舒 晓萍
Published: April 2020

随着社会的发展和进步,企业规模越发庞大,企业用水量日益增加,水资源越发短缺。其中作为工业欠发达地区的缙云县,工业冷却水用量约占整个工业用水量的50%～60%。因此发展新型节水技术,提高工业冷却水的循环利用率是缓解全县水资源紧张的一个重大举措,可以使有限的水资源得到最大程度的重复利用。本文重点针对工业循环冷却水节水技术展开分析和研究,通过实地调查,有效提出了相应的节水技术方案,对提高工业循环冷却水的节水效果有着重要的意义。
简析中小型水利工程施工现场安全生产可视化建设
Authors: 赵 亮
Published: April 2020

改革开放以来,我国在中小型水利工程建设方面投入力度不断增强,呈现出点多面广和安全隐患多的特点,很多地区由于管理不当造成了中小型水利工程不同程度的损坏,缩短工程使用寿命,并出现了不同程度的资源损耗。基于此,针对其中的问题,推动中小型水利工程施工现场安全生产可视化建设,选择合适的工具降低安全隐患,对于维护施工安全具有重要作用。本文就此着手阐述,提出有效措施来达成这一目标。
水利工程中土石坝筑坝关键技术分析
Authors: 史 金忠
Published: April 2020

在当前我国水利工程施工整体质量得到稳定提升的背景下,筑坝工程中的土石坝技术作为一项重要技术手段也得到更为广泛的应用。在水利工程的施工过程中,土石坝施工技术的合理应用不仅能实现对筑坝工程施工质量的提升,同时对于延长工程使用寿命,降低工程建设成本也有着重要帮助,因此这一技术的应用也得到了越来越多水利施工企业的关注和重视。随着近年来我国社会发展水平的提升,水利事业也得到了全新的发展契机,所以要想在此种情况下进行稳定发展,我们更应该加强对先进科学技术手段的应用,只有对各个环节实现精准把控,才能为后续水利工程施工质量的提升奠定有效帮助。
水工大体积混凝土温控研究
Authors: 袁 三涛
Published: April 2020

随着我国各项技术的不断发展,建筑行业领域有着突飞猛进的提高。同时,混凝土建筑物的修建也越加迅猛。虽然混凝土建筑修建过程比较迅猛,但是混凝土的温度却难以控制,从而严重阻碍了混凝土建筑后期工作的顺利开展及混凝土建筑质量的保障。并且,混凝土建筑物与混凝土的问题有着密不可分的联系,如果混凝土建筑的温度存在着一定的问题,混凝土建筑的质量就会大幅度下降,严重时混凝土建筑物将会无法使用。因此,相关的施工人员要从问题的根本出发,根据混凝土温度所存在的问题,制定科学合理的解决方案。本篇文章主要阐述了混凝土建筑过程当中温度存在的问题及解决方案,为相关的工作人员提供参考。
工程地质勘察中的水文地质危害及其对策
Authors: 王 倩
Published: April 2020

在工程地质勘察过程中,水文地质勘察是其中的关键,在很大程度上影响着建筑工程地质勘察和施工的顺利实施,地质勘察部门需要认真勘察并研究工程所在地区的水文地质情况,掌握相应的水文参数,深入分析水文地质中的危害,并采取相应的措施进行改善,为建筑工程项目建设的顺利实施提供保障。在现代化社会的发展中,社会各界更加明确工程地质勘察中的水文地质危害,文章主要对工程地质勘察中的水文地质危害及对策进行了分析。
探析水利工程施工中的生态环境问题及对策
Authors: 郑 媛芳
Published: April 2020

随着经济社会快速发展,水利工程建设也迎来了全新的发展机遇,因此提高水利工程建设水平与质量具有十分重要的意义。水利工程建设施工不同程度对周边生态环境带来影响与破坏,如果不能从生态环境保护强化水利工程施工管理,将会严重降低水利工程的社会、经济价值。本文简要阐述了水利工程施工中的生态环境问题,并提出了切实可行的解决对策,推动流域水利工程建设管理水平全面提升。
新时期水库移民安置模式探析
Authors: 徐 刚
Published: April 2020

为了满足社会发展需要,兴建水库可以实现水资源合理配置与利用,减少水资源浪费,但是如果水库移民安置问题未能得到有效解决,将造成一系列遗留问题,威胁社会和谐、稳定,影响工程建设进度。本文就新时期水库移民安置模式进行探究,针对其中的问题,对水库移民安置模式进行探索和创新,推动我国水利工程建设和发展。
农村饮用水的不安全因素研究以及相关的优化对策
Authors: 王 晓飞
Published: April 2020

我国是一个人口大国,虽然地大物博,水资源总量排名较高,但是,人均水资源却只占世界人均水资源的20%。因此,近几年来,我国也逐渐的开始重视对饮用水水源的保护,致力于解决国民的饮水安全问题。基于此,文章就农村饮用水的不安全因素以及相关的优化措施方面进行了简单分析,希望对提高我国农村饮用水的安全性,促进我国的经济社会可持续发展等方面有所启示和帮助。
Інноваційний інструментарій структурної політики управління розвитком аграрного сектору економіки
Authors: Kovalova O. V.
Published: April 2020

Статтю присвячено дослідженню питань інноваційного інструментарію структурної політики управління розвитком аграрного сектору економіки.

Метою статті є дослідження та застосування інноваційних інструментів структурної політики управління розвитком аграрного сектору економіки.

Наукова новизна. Визначено, що існує взаємозв’язок між типом економічних відносин та моделлю управління, періодичністю процесів ускладнення та спрощення систем управління, пов’язаних із базовим розподілом праці. При спрощені (скороченні) функцій державного управління на перший план виходять альтернативні організації. Тому актуалізуються принципи належного врядування як елемент організаційно-управлінської системи політики, притаманний сучасним системам державного управління. Визначено логічні підсистеми структурної політики управління розвитком аграрного сектору економіки. Розроблено та обґрунтовано напрями і стратегічні пріоритети модернізаційної структурної політики в аграрному секторі економіки України.

Висновки. Забезпечення сталого розвитку аграрного сектору – визначальний пріоритет, який повинна враховувати держава при розробці і здійсненні структурної політики. Реалізація концепції сталого розвитку за рахунок раціонального використання потенціалу сільського сектору, сільського господарства й суміжних галузей має стати головною компонентою національного соціально-економічного зростання. Без відповідної структурної політики розвитку аграрного сектору рух в заданому напрямі є проблематичним. На такому баченні перспективи сьогодні наполягає усе передове світове співтовариство тому, що до аграрного сектору сформовані принципи економічної, екологічної та соціальної сталості. Усі вони пов’язані з природою та діяльністю людини, а сільське господарство – частина природи, яка задіяна у формуванні системи забезпечення головною компонентою людського існування – продовольством. Сталий розвиток аграрного сектору економіки нерозривно пов'язаний із забезпеченням сталого розвитку сільських територій. І тут необхідним є врахування зарубіжного, зокрема Європейського досвіду вирішення цієї проблеми. Вирішення даного питання вважаємо стратегічним для забезпечення конкурентоспроможного розвитку агропродовольчого комплексу та сільських територій. Стратегічні пріоритети сталого розвитку аграрного сектору економіки мають узгоджуватися з глобальними рішеннями, які прийняті світовою спільнотою, зокрема Цілями сталого розвитку 2030.
Activation of Nrf2 by Ischemic Preconditioning and Sulforaphane in Renal Ischemia/Reperfusion Injury: a Comparative Experimental Study
Authors: SHOKEIR A. A. , BARAKAT N. , HUSSEIN A. M. , AWADALLA A. , HARRAZ A. M. , KHATER S. , HEMMAID K. , KAMAL A. I.
Published: April 2020

Objectives of the study were to investigate impact of ischemic preconditioning (Ipre) and sulforaphane (SFN) and combination of them on nuclear factor 2 erythroid related factor 2 (Nrf2) gene and its dependent genes, heme oxygenase-1 (HO1) and NADPH-quinone oxidoreductase1 (NQO-1) and inflammatory cytokines TNF-α, IL1β, and intercellular adhesion molecule-1 (ICAM1) and caspase-3 in renal ischemia/reperfusion (I/R) injury. Ninety male Sprague Dawely rats were classified into 5 groups (each consists of 18 rats): sham, control, Ipre, sulforaphane and Sulfo+Ipre. Each group was subdivided into 3 subgroups each containing 6 rats according to time of harvesting kidney and taking blood samples; 24 h, 48 h, and 7 days subgroups. Renal functions including serum creatinine, BUN were measured at basal conditions and by the end of experiment. Expression of Nrf2, HO-1, NQO-1, TNF-α, IL-1β, and ICAM-1 was measured by real time PCR in kidney tissues by the end of experiment. Also, immunohistochemical localization of caspase-3 and chemical assay of malondialdehyde (MDA), GSH and SOD activity were measured in kidney tissues. Both Ipre and SFN improved kidney functions, enhanced the expression of Nrf2, HO-1, and NQO-1, attenuated the expression of inflammatory (TNF-α, IL-1, and ICAM-1) and apoptotic (caspase-3) markers. However, the effect of sulforaphane was more powerful than Ipre. Also, a combination of them caused more improvement in antioxidant genes expression and more attenuation in inflammatory genes but not caspase-3 than each one did separately. Sulforaphane showed more powerful effect in renoprotection against I/R injury than Ipre as well as there might be a synergism between them at the molecular but not at the function level.
Possible Intracellular Regulators of Female Sexual Maturation
Authors: KOLESAROVA A. , SIROTKIN A. V. , MELLEN M. , ROYCHOUDHURY S.
Published: April 2020

Protein kinases, transcription factors and other apoptosis- and proliferation-related proteins can regulate reproduction, but their involvement in sexual maturation remains to be elucidated. The general aim of the in vivo and in vitro experiments with porcine ovarian granulosa cells was to identify possible intracellular regulators of female sexual maturation. For this purpose, proliferation (expression of proliferating cell nuclear antigen – PCNA, mitogen-activated protein kinases – ERK 1,2 related MAPK and cyclin B1), apoptosis (expression of the apoptotic protein Bax and apoptosis regulator Bcl-2 protein), expression of some protein kinases (cAMP dependent protein kinase – PKA, cGMP-dependent protein kinase – PKG, tyrosine kinase – TK) and cAMP responsive element binding protein 1 (CREB-1) was examined in granulosa cells isolated from ovaries of immature and mature gilts. Expression of PCNA, ERK1,2 related MAPK, cyclin B1, Bcl-2, Bax, PKA, CREB-1, TK and PKG in porcine granulosa cells were detected by immunocytochemistry. Sexual maturation was associated with significant increase in the expression of Bcl-2, Bax, PKA, CREB-1 and TK and with decrease in the expression of ERK1,2 related MAPK, cyclin B1 and PKG in granulosa cells. No significant difference in PCNA expression was noted. The present data obtained from in vitro study indicate that sexual maturation in females is influenced by puberty-related changes in porcine ovarian signaling substances: increase in Bcl-2, Bax, PKA, CREB-1, TK and decrease in ERK1,2 related MAPK, cyclin B1 and PKG. It suggests that these signaling molecules could be potential regulators of porcine sexual maturation.
Involvement of Actin Microfilament in Regulation of Pacemaking Activity Increased by Hypotonic Stress in Cultured ICCs of Murine Intestine
Authors: WANG Z. Y. , HUANG X. , LIU D. H. , LU H. L. , KIM Y. C. , XU W. X.
Published: April 2020

Distension is a regular mechanical stimulus in gastrointestinal (GI) tract. This study was designed to investigate the effect of hypotonic stress on pacemaking activity and determine whether actin microfilament is involved in its mechanism in cultured murine intestinal interstitial cells of Cajal (ICCs) by using whole-cell patch-clamp and calcium imaging techniques. Hypotonic stress induced sustained inward holding current from the baseline to –650±110 pA and significantly decreased amplitudes of pacemaker current. Hypotonic stress increased the intensity of basal fluorescence ratio (F/F0) from baseline to 1.09±0.03 and significantly increased Ca2+ oscillation amplitude. Cytochalasin-B (20 μM), a disruptor of actin microfilaments, significantly suppressed the amplitudes of pacemaker currents and calcium oscillations, respectively. Cytochalasin-B also blocked hypotonic stress-induced sustained inward holding current and hypotonic stress-induced increase of calcium oscillations. Phalloidin (20 μM), a stabilizer of actin microfilaments, significantly enhanced the amplitudes of pacemaker currents and calcium oscillations, respectively. Despite the presence of phalloidin, hypotonic stress was still able to induce an inward holding current and increased the basal fluorescence intensity. These results suggest that hypotonic stress induces sustained inward holding current via actin microfilaments and the process is mediated by alteration of intracellular basal calcium concentration and calcium oscillation in cultured intestinal ICCs.
Role of Tetraspanin CD9 Molecule in Fertilization of Mammals
Authors: JANKOVIČOVÁ J. , SIMON M. , ANTALÍKOVÁ J. , CUPPEROVÁ P. , MICHALKOVÁ K.
Published: April 2020

Fertilization process is a very clever and unique process comprising some essential steps resulting in formation of zygote. Tetraspanin CD9 is considered to be a serious candidate molecule participating in these events. The importance of CD9 has been discussed in relation to acrosome reaction, sperm-binding, sperm-penetration, sperm-egg fusion and eventually, egg activation. The abundant expression of CD9 oocyte plasma membrane and the presence of CD9-containing vesicles in the perivitelline space of intact oocytes have been confirmed. Despite the fact that majority of authors analyzed CD9 expressed on oocytes, several studies considered the function of sperm CD9, too. To understand CD9 involvement, various conditions of in vitro fertilization (IVF) assays using polyclonal as well as monoclonal antibodies or knockout mice were carried out. However, ambiguous data have been obtained about the importance of CD9 in sperm-egg binding or fusion. Although the current findings did not prove any hypothesis, the indispensable role of CD9 in fertilization process was not excluded and the precise role of CD9 remains unexplained.
α-actin Down Regulation and Perforin Loss in Uterine Natural Killer Cells From LPS-Treated Pregnant Mice
Authors: ZAVAN B. , DO AMARANTE-PAFFARO A. M. , PAFFARO V. A.
Published: April 2020

One of the most abundant immunologic cell types in early decidua is the uterine natural killer (UNK) cell that despite the presence of cytoplasmic granules rich in perforin and granzymes does not degranulate in normal pregnancy. UNK cells are important producers of angiogenic factors that permit normal dilation of uterine arteries to provide increased blood flow for the growing feto-placental unit. Gram-negative bacteria lipopolysaccharide (LPS) administration can trigger an imbalance of pro-inflammatory and anti-inflammatory cytokines impairing the normal immune cells activity as well as uterine homeostasis. The present study aimed to evaluate by immunohistochemistry the reactivity of perforin and α-actin on UNK cell from LPS-treated pregnant mice. For the first time, we demonstrate that LPS injection in pregnant mice causes α-actin down regulation, concomitantly with perforin loss in UNK cells. This suggests that LPS alters UNK cell migration and activates cytotoxic granule release.
DAYA ANTIBAKTERI SEDIAAN GEL EKSTRAK ETANOL DAUN MURBEI (Morus alba L.) SEBAGAI OBAT LUKA PADA KULIT TERHADAP Staphylococcus aureus
Authors: Asri Muhammad , Bahar Fahril
Published: April 2020

A research concerning the antibacterial effect of mulberry leaves (Morusalba L.) ethanol extract gel as cure of skin wounds against Staphylococcus aureus bacteria.This research is aimed to know the antibacterial effect of mulberry leaves (Morusalba L.) ethanol extract gel as cure of skin wounds against Staphylococcus aureus bacteria and determine inhibitor concentration minimum value. It was an experimental research which conducted in the laboratory to test the antibacterial effect of mulberry leaves (Morusalba L.) ethanol extract gel as cure of skin wounds against Staphylococcus aureus bacteria.            The result of observation showed that 1% concentration produced diameter of resistance zone average as 0.83 mm, 3% concentration were 0.97 mm and 5% concentration were 1.13 mm. It can be concluded that; 1) mulberry leaves ethanol extract gel stock on 1%, 3%, and 5% concentration have a weak resistance based on Antimicrobial resistor category ;2) inhibitor concentration minimum value of 1% concentration, was the lowest concentration that still giving antibacterial effect to against of Staphylococcus aureus bacterial
PENETAPAN KADAR FENOLIK TOTAL FRAKSI POLR DAN NONPOLAR DAUN RAMBAI LAUT (Sonneratia caseolaris L.) DENGAN METODE SPEKTROFOTOMETRI UV-Vis
Authors: Jubaidah Siti , Sundu Reksi , Sabriningsih Nur
Published: April 2020

Rambai laut (Soneratia caseolaris L.) is a type of tree that grows in riverbank swamps or mangrove forests which contains one of the secondary metabolites in the form of phenolic compounds. The purpose of this study was to determine the total phenolic content of polar and nonpolar fractions of sea rambai leaves using UV-Vis spectrophotometry method.

Research conducted is non-experimental. The stages of the study included the determination of plants, sampling, making samples, making extracts, determining the water content of extracts, fractionation using polar and nonpolar fractions, phytochemical screening test of ethanol extract and fractionation results and determination of total phenolic levels by UV-Vis spectrophotometry.

The results showed that rambai laut leaf in polar fraction had a total phenolic content of 213.49 ± 1.2227 mg GAE / g, which means that in each gram the polar fraction is equivalent to 213.49 mg of gallic acid, whereas in the nonpolar fraction of rambai laut leaves is obtained 55.79 ± 1.0809 mg GAE / g which means that in each gram the nonpolar fraction is equivalent to 55.79 mg of gallic acid. Total phenolic content is greater in the polar fraction.
FORMULASI NANOEMULGEL EKSTRAK KULIT MANGGIS (Garcinia Mangostana L.)
Authors: Damayanti Hilda , Wikarsa Saleh , Jafar Garnadi
Published: April 2020

Antioxidant-containing cosmetic has antiaging therapy that can inhibit the free radical formation. Mangosteen peel extract has very strong antioxidant activity. To enhance the effect and comfortness of mangosteen peel extract use on the skin, it could be made into nanoemulgel. The article provides the information about method of preparation and evaluation of nanoemulsion-gel. The purpose of this study was to formulate a stable microemulgel of mangosteen peel extractusing halal materials declared halal according to Islamic Shari’a.  the materials used don’t contain carrion, blood, pig and/ animals that don’t conform to Islamic Shari’a. Microemulgel mangosteen peel extract was made by varying plantacare® 1200 UP concentration as cosurfactant (5, 10, 15, 20 and 25%) in the microemulsion and it was incorporated into the gel base. Evaluations were included the antioxidant activity test and organoleptic, pH, viscosity, stability, particle size analysis and panelist test. The antioxidant activity determined by DPPH method showed that IC50 value of mangosteen peel extract was 5.54 ppm. The third microemulsion formula containing cosurfactant of 15% resulted in the best results in that the parameter of the product can be penetrated by ray laser was at particle size of  23.65 nm, was determined by tranmission Electron Microscopy (TEM). Microemulgel containing Viscolam® MAC 10 of seven percent gave the stable formula proofed by freeze thaw and sentrifuga test. The five microemulgel formulations were stable.
ISOLASI DAN UJI AKTIVITAS DAYA HAMBAT EKSTRAK ETANOL BIJI PINANG (Areca catechu L.) TERHADAP BAKTERI PADA LIDAH
Authors: Djohari Meiriza , Putri Wulandari Yulia , Pratiwi Erniza
Published: April 2020

Betel  nut (Areca catechu L.) is a natural material that has antibacterial activity in treating diseased on teeth, halitosis and can inhibit of dental caries. The objective of this research is type of bacteria based on morphology, the results of bacterial identification tests on the tongue and to determine the effect of the inhibitory power of betel nut ethanol extract (Areca catechu L.) to bacteria of the tongue. Antibacterial activity testing using disc diffusion method with various concentrations of 10%, 20% and 30%. Secondary metabolite contents  are alkaloid, terpenoid and flavonoid.The results obtained from the identification test based on the color of bacteria Branhamella catarrhalis, Staphylococcus epidermidis and Straphylococcus aureus. Ethanol extract of areca nut (Areca catechu L.) is better to inhibiting the growth of Staphylococcus epidermidis and Staphylococcus aureus compared to Branhamella catarrhalis. Based on Two Way Anova statistical data on inhibitory zone diameters, there were differences between the concentration groups of 10%, 20% and 30% and there were significant differences in the inhibitory activity of Branhamella catarrhalis bacteria compared to Staphylococcus epidermidis and Straphylococcus aureus bacteria but not significantly different between Staphylococcus epidermidis and Straphylococcus aureus bacteria
FORMULASI DAN EFEKTIFITAS SAMPO EKSTRAK BUAH PEDADA (Sonneratia caseolaris L) SEBAGAI ANTIKETOMBE TERHADAP Candida albicans
Authors: Sambodo Dwi Kurniawati , Yani Lisa Erie
Published: April 2020

Half of the world's population has been affected by dandruff, especially those aged 15 to 50 years. Dandruff is one of the problems on the scalp with common symptoms of scales (exfoliation of dead skin), itching of the scalp and redness around the scalp. Dandruff can be caused by an oily scalp, hormones or fungus. One of the fungi that cause dandruff is Candida albicans. Based on research that has been done, secondary metabolite compounds of methanol extract of peada fruit peel which is a pure white compound in the form of powder are identified as alkaloid compounds. In general, alkaloids can be used as an antifungal. Anti-dandruff shampoo is a dosage that is often used daily and can be used to treat and prevent scalp from excessive dandruff where this shampoo contains active antifungal agents which are effective as an anti-dandruff. Pedada fruit was extracted using multilevel maceration method with methanol as a solvent. Pedada fruit extract shampoo is formulated with variations in extract concentration into 3 formulas. Shampoo was tested for physical properties and effectiveness of anti-dandruff using the Kirby-Bauer Diffusion method with PDA media. From the results obtained it can be concluded that the physical properties of the three formulas of pedada fruit extract shampoo (Sonnerratia caseolaris L) include organoleptic, pH , high foam , cycling test, and viscosity produced good physical properties in accordance with the standard test of the physical properties of shampoo, however Homogeneity test results show that formula III shampoo is less homogeneous. All three shampoo formulas are effective as anti-dandruff against the fungus Candida albicans.
Estimation of Reduced Air Emissions as a Result of the Implementation of the Measure to Reduce Burned Sugarcane in Thailand
Authors: Junpen Agapol , Pansuk Jirataya , Garivait Savitri
Published: April 2020

In the middle of 2019, Thailand introduced an urgent measure to solve the haze and fine particles problem caused by preharvest sugarcane. The measure is to eradicate the burning of sugarcane before harvesting within the next four years. This study focuses on estimation of future reduced air emissions from the implementation of this measure. The study results showed that without the implementation of the measure in the production season 2022/23, the amount of burned sugarcane will be as high as 102.4 ± 1.0 Mt/yr, resulting in the release of small particulate matter of 2.5 microns or less in diameter (PM2.5) emissions into the atmosphere at 30.6 ± 8.6 kt/yr, which is about a 21% increase in emissions from the 2018/19 season. However, with the government’s enforcement of the measure to reduce burned sugarcane, the amount of air emissions will immediately be reduced in the first year. In order to effectively implement the sugarcane burning reduction measure, in which the harvesting methods will change from cutting burned sugarcane to cutting fresh sugarcane with human labor and with machinery, the government must provide financial support, to farmers, of approximately 3.3 USD per ton of fresh sugarcane for the use of human labor and 2.9 USD per ton of fresh sugarcane for the use of machinery. The costs of investment to reduce PM2.5 emissions can be calculated in the range of 9.7−11.0 million USD per kiloton of PM2.5.
PD-L1–PD-1 Pathway in the Pathophysiology of Multiple Myeloma
Authors: Tamura Hideto , Ishibashi Mariko , Sunakawa-Kii Mika , Inokuchi Koiti
Published: April 2020

PD-L1 expressed on tumor cells contributes to disease progression with evasion from tumor immunity. Plasma cells from multiple myeloma (MM) patients expressed higher levels of PD-L1 compared with healthy volunteers and monoclonal gammopathy of undetermined significance (MGUS) patients, and its expression is significantly upregulated in relapsed/refractory patients. Furthermore, high PD-L1 expression is induced by the myeloma microenvironment and PD-L1+ patients with MGUS and asymptomatic MM tend to show disease progression. PD-L1 expression on myeloma cells was associated with more proliferative potential and resistance to antimyeloma agents because of activation of the Akt pathway through PD-1-bound PD-L1 in MM cells. Those data suggest that PD-L1 plays a crucial role in the disease progression of MM.
Cooperation as the Secret Ingredient in the Recipe to Foster Internal Technological Eco-Innovation in the Agri-Food Industry
Authors: Rabadán Adrián , Triguero Ángela , Gonzalez-Moreno Ángela
Published: April 2020

Although eco-innovation in the agri-food sector is receiving increasing amounts of attention, there is a lack of information about the specific conditions that encourage firms to develop eco-innovation strategies internally. Our empirical method relies on the data of Spanish firms operating in the agri-food sector, and uses the Qualitative Comparative Analysis (QCA). Specifically, we identify the recipes of antecedent conditions that effectively foster the internal development of technological eco-innovation, and then we analyze whether differences exist in the internal development of product and process eco-innovations. The results show that different combinations of conditions can yield internally developed eco-innovation, but all of them indicate that cooperation with stakeholders is the key to fostering technological eco-innovation in this industry. This conclusion encourages the creation of policies and incentives to promote cooperation in order to improve the sustainability of the sector.
Antimicrobial Materials with Lime Oil and a Poly(3-hydroxyalkanoate) Produced via Valorisation of Sugar Cane Molasses
Authors: Basnett Pooja , Marcello Elena , Lukasiewicz Barbara , Nigmatullin Rinat , Paxinou Alexandra , Ahmad Muhammad Haseeb , Gurumayum Bhavana , Roy Ipsita
Published: April 2020

A medium chain-length polyhydroxyalkanoate (PHA) was produced by Pseudomonas mendocina CH50 using a cheap carbon substrate, sugarcane molasses. A PHA yield of 14.2% dry cell weight was achieved. Chemical analysis confirmed that the polymer produced was a medium chain-length PHA, a copolymer of 3-hydroxyoctanoate and 3-hydroxydecanoate, P(3HO-co-3HD). Lime oil, an essential oil with known antimicrobial activity, was used as an additive to P(3HO-co-3HD) to confer antibacterial properties to this biodegradable polymer. The incorporation of lime oil induced a slight decrease in crystallinity of P(3HO-co-3HD) films. The antibacterial properties of lime oil were investigated using ISO 20776 against Staphylococcus aureus 6538P and Escherichia coli 8739, showing a higher activity against the Gram-positive bacteria. The higher activity of the oil against S. aureus 6538P defined the higher efficiency of loaded polymer films against this strain. The effect of storage on the antimicrobial properties of the loaded films was investigated. After one-year storage, the content of lime oil in the films decreased, causing a reduction of the antimicrobial activity of the materials produced. However, the films still possessed antibacterial activity against S. aureus 6538P.
Production of Biodiesel and High-Protein Feed from Fish Processing Wastes Using In Situ Transesterification
Authors: Zhang Tongdong , Du Beiyan , Lin Yuexu , Zhang Min , Liu Yueliang
Published: April 2020

Preparation of biodiesel using in situ transesterification has been extensively conducted for agricultural, microbial and algal biomass, while few works have been performed using aquatic animal tissue. In this work, fish processing wastes were collected to perform in situ transesterification using grass carp (Ctenopharyngodon idellus) biomass as a representative with which to optimize the reaction conditions. Under the optimum condition, the highest biodiesel purity reached up to 100% for sea bass wastes, which is higher than the 96.5% specified in the EN 14214-2008. The in situ method proposed here has the potential to save significant costs in biodiesel production compared to conventional methods, which usually require high-cost pretreatment of the raw materials. Additionally, the waste residue byproduct produced has a high protein content, and therefore the potential to be used for high-protein feed. This study is expected to inspire new strategies to prepare biodiesel and high-protein feed simultaneously from aquatic animal biomass using the novel in situ transesterification.
Mono-, Di- and Tetra-iron Complexes with Selenium or Sulphur Functionalized Vinyliminium Ligands: Synthesis, Structural Characterization and Antiproliferative Activity
Authors: Agonigi Gabriele , Batchelor Lucinda K. , Ferretti Eleonora , Schoch Silvia , Bortoluzzi Marco , Braccini Simona , Chiellini Federica , Biancalana Lorenzo , Zacchini Stefano , Pampaloni Guido , Sarkar Biprajit , Dyson Paul J. , Marchetti Fabio
Published: April 2020

A series of diiron/tetrairon compounds containing a S- or a Se-function (2a–d, 4a–d, 5a–b, 6), and the monoiron [FeCp(CO){SeC1(NMe2)C2HC3(Me)}] (3) were prepared from the diiron μ-vinyliminium precursors [Fe2Cp2(CO)( μ-CO){μ-η1: η3-C3(R’)C2HC1N(Me)(R)}]CF3SO3 (R = R’ = Me, 1a; R = 2,6-C6H3Me2 = Xyl, R’ = Ph, 1b; R = Xyl, R’ = CH2OH, 1c), via treatment with S8 or gray selenium. The new compounds were characterized by elemental analysis, IR and multinuclear NMR spectroscopy, and structural aspects were further elucidated by DFT calculations. The unprecedented metallacyclic structure of 3 was ascertained by single crystal X-ray diffraction. The air-stable compounds (3, 4a–d, 5a–b, 6) display fair to good stability in aqueous media, and thus were assessed for their cytotoxic activity towards A2780, A2780cisR, and HEK-293 cell lines. Cyclic voltammetry, ROS production and NADH oxidation studies were carried out on selected compounds to give insights into their mode of action.
Exercise Affects Blood Glucose Levels and Tissue Chromium Distribution in High-Fat Diet-Fed C57BL6 Mice
Authors: Chang Geng-Ruei , Hou Po-Hsun , Chen Wen-Kai , Lin Chien-Teng , Tsai Hsiao-Pei , Mao Frank Chiahung
Published: April 2020

Obesity is commonly associated with hyperglycemia and type 2 diabetes and negatively affects chromium accumulation in tissues. Exercise prevents and controls obesity and type 2 diabetes. However, little information is available regarding chromium changes for regulating glucose homeostasis in high-fat diet (HFD)-fed animals/humans who exercise. Therefore, this study explored the effects of exercise and whether it alters chromium distribution in obese mice. Male C57BL6/J mice aged 4 weeks were randomly divided into two groups and fed either an HFD or standard diet (SD). Each group was subgrouped into two additional groups in which one subgroup was exposed to treadmill exercise for 12 weeks and the other comprised control mice. HFD-fed mice that exercised exhibited significant lower body weight gain, food/energy intake, daily food efficiency, and serum leptin and insulin levels than did HFD-fed control mice. Moreover, exercise reduced fasting glucose and enhanced insulin sensitivity and pancreatic β-cell function, as determined by homeostasis model assessment (HOMA)-insulin resistance and HOMA-β indices, respectively. Exercise also resulted in markedly higher chromium levels within the muscle, liver, fat tissues, and kidney but lower chromium levels in the bone and bloodstream in obese mice than in control mice. However, these changes were not noteworthy in SD-fed mice that exercised. Thus, exercise prevents and controls HFD-induced obesity and may modulate chromium distribution in insulin target tissues.
Oligomerization and Conformational Change Turn Monomeric β-Amyloid and Tau Proteins Toxic: Their Role in Alzheimer’s Pathogenesis
Authors: Penke Botond , Szűcs Mária , Bogár Ferenc
Published: April 2020

The structural polymorphism and the physiological and pathophysiological roles of two important proteins, β-amyloid (Aβ) and tau, that play a key role in Alzheimer’s disease (AD) are reviewed. Recent results demonstrate that monomeric Aβ has important physiological functions. Toxic oligomeric Aβ assemblies (AβOs) may play a decisive role in AD pathogenesis. The polymorph fibrillar Aβ (fAβ) form has a very ordered cross-β structure and is assumed to be non-toxic. Tau monomers also have several important physiological actions; however, their oligomerization leads to toxic oligomers (TauOs). Further polymerization results in probably non-toxic fibrillar structures, among others neurofibrillary tangles (NFTs). Their structure was determined by cryo-electron microscopy at atomic level. Both AβOs and TauOs may initiate neurodegenerative processes, and their interactions and crosstalk determine the pathophysiological changes in AD. TauOs (perhaps also AβO) have prionoid character, and they may be responsible for cell-to-cell spreading of the disease. Both extra- and intracellular AβOs and TauOs (and not the previously hypothesized amyloid plaques and NFTs) may represent the novel targets of AD drug research.
Directing 2D-Coordination Networks: Combined Effects of a Conformationally Flexible 3,2′:6′,3″-Terpyridine and Chain Length Variation in 4′-(4-n-Alkyloxyphenyl) Substituents
Authors: Rocco Dalila , Prescimone Alessandro , Constable Edwin C. , Housecroft Catherine E.
Published: April 2020

The synthesis and characterization of 4′-(4-n-propoxyphenyl)-3,2′:6′,3″-terpyridine is described. Five 2D-coordination networks have been isolated by crystal growth at room temperature from reactions of Co(NCS)2 with 4′-(4-n-alkyloxyphenyl)-3,2′:6′,3″-terpyridines in which the n-alkyl group is ethyl, n-propyl, n-butyl, n-pentyl and n-hexyl in ligands 2–6, respectively. The single-crystal structures of [{Co(2)2(NCS)2}.0.6CHCl3]n, [{Co(3)2(NCS)2}.4CHCl3.0.25H2O]n, [{Co(4)2(NCS)2}.4CHCl3]n, [Co2(5)4(NCS)4]n and [Co(6)2(NCS)2]n have been determined, and powder X-ray diffraction has demonstrated that the single-crystal structures are representative of the bulk materials. Each compound possesses a (4,4) net with Co centres as 4-connecting nodes. For the assemblies containing 2, 3 and 4, the (4,4) net comprises two geometrically different rhombuses, and the nets pack in an ABAB... arrangement with cone-like arrangements of n-alkyloxyphenyl groups being accommodated in a similar unit in an adjacent net. An increase in the n-alkyloxy chain length has two consequences: there is a change in the conformation of the 3,2′:6′,3″-tpy metal-binding domain, and the (4,4) net comprises identical rhombuses. Similarities and differences between the assemblies with ligands 2–6 and the previously reported [{Co(1)2(NCS)2}.3MeOH]n and [{Co(1)2(NCS)2}.2.2CHCl3]n in which 1 is 4′-(4-methoxyphenyl)-3,2′:6′,3″-terpyridine are discussed. The results demonstrate the effects of combining a variable chain length in the 4′-(4-n-alkyloxyphenyl) substituents of 3,2′:6′,3″-tpy and a conformationally flexible 3,2′:6′,3″-tpy metal-binding domain.
Quasi-Periodic Dendritic Metasurface for Integral Operation in Visible Light
Authors: Chen Huan , An Di , Zhao Xiaopeng
Published: April 2020

A reflective metasurface model composed of silver dendritic units is designed in this study. The integral property of this metasurface, which consists of an upper layer of dendritic structures, a silica spacer, and a bottom silver substrate was demonstrated at visible wavelengths. The simulation results revealed that the metasurface can perform integral operation in the yellow and red bands; this can be easily generalized to the infrared and communication bands by scaling the transverse dimensions of this metasurface. A dendritic metasurface sample responding to red light was prepared via the bottom-up electrochemical deposition method. The integral operation property of the sample was verified experimentally. This dendritic metasurface, which can perform integral operation in visible light, can be used for big data processing technology, real-time signal processing, and beam shaping, and provides a new method for miniaturized and integrated all-optical signal processing systems.
A Pilot Study on the Effect of Anti-Thrombopoietin Antibody on Platelet Count in Patients with Type 2 Diabetes
Authors: Fukuda Takuya , Hamaguchi Masahide , Osaka Takafumi , Hashimoto Yoshitaka , Ushigome Emi , Asano Mai , Yamazaki Masahiro , Fukuda Eriko , Yamaguchi Kei , Ogawa Koji , Goshima Naoki , Fukui Michiaki
Published: April 2020

Thrombopoietin (THPO) is a circulatory cytokine that plays an important role in platelet production. The presence of anti-THPO antibody relates to thrombocytopenia and is rarely seen in hematopoietic and autoimmune diseases. To date, there had been no reports that focused on the anti-THPO antibody in patients with type 2 diabetes mellitus (T2DM). To evaluate prevalence of the anti-THPO antibody in patients with T2DM and the relationship between anti-THPO antibody and platelet count, a cross-sectional study was performed on 82 patients with T2DM. The anti-THPO antibody was measured by ELISA using preserved sera and detected in 13 patients. The average platelet count was significantly lower in patients with the anti-THPO antibody than in those without the anti-THPO antibody. Multivariate linear regression analyses showed a significant relationship between the anti-THPO antibody and platelet count, after adjusting for other variables. To our best knowledge, this was the first report on the effect of the anti-THPO antibody on platelet count in patients with T2DM. Further investigation is needed to validate the prevalence and pathological significance of the anti-THPO antibody in patients with T2DM.