bluuebunny/crossref_metadata_2025 huggingface dataset:
Safety and immunogenicity of the yellow fever vaccine for patients with end-stage renal disease
Authors: Distenhreft Jesiree Iglésias Quadros , Couto-Lima Dinair , Andreazzi Cecilia Siliansky de , Thomazini Juliana Feu Rosa Carrera , Vasconcellos Filho Lauro Monteiro , Falqueto Aloísio , Luchi Weverton Machado
Published: August 2024

Abstract Introduction: In December 2016, an outbreak of sylvatic yellow fever (YF) occurred in the non-endemic areas of the south-eastern region of Brazil. The immune response to the yellow fever vaccine and its safety in individuals with chronic kidney disease (CKD) living in YF-endemic regions are not thoroughly understood. The objective of this study is to assess the incidence of adverse events and the serological response after primary vaccination with the 17DD-YF vaccine in CKD patients undergoing dialysis. Methods: This was a multicenter, retrospective cohort study involving 223 individuals with CKD who were on dialysis after primary vaccination against YF. Clinical and epidemiologic characteristics were collected and the vaccine adverse event (VAE) were assessed. Around 35 months after vaccination, the serological response was evaluated in 71 (32%) patients using neutralization tests. Results: No serious VAE occurred in any patient. Local reactions were reported in 13 individuals (5.8%), while 6 (2.7%) reported generalized systemic reactions and 205 (91.9%) did not display any VAE. No clinical or epidemiologic characteristic predicted the occurrence of VAE. Adequate serological response was found in 38% of participants and none of the clinical or epidemiological characteristics were associated with immunogenicity. Conclusion: The outcomes of our study suggest that the yellow YF vaccine is well-tolerated in CKD patients undergoing dialysis, but it does not induce adequate immune response. Future research should focus on evaluating both cellular and humoral immune responses following administration of various doses of the YF vaccine.
Maternity hospital in the city of Natal in Rio Grande do Norte as a military hospital between 1941-1950: a qualitative study
Authors: Carlos Djailson José Delgado , Queirós Paulo Joaquim Pina , Lino Cristiane Ribeiro de Melo
Published: August 2024

Abstract Objective to analyze the circumstances in which the Maternidade de Natal functioned as a military hospital (1941-1950). Method a qualitative, social history study, elaborated with documentary and bibliographical sources. Data were organized and analyzed according to thematic content analysis. Results the Maternidade de Natal operation required commitment, coordination and strategies from the Hospital Assistance Society regarding financial difficulties, transfer of the building to the Ministry of War, during World War II, and finally, the return and its inauguration. This context transformed the daily life of Natal and, thus, the military hospital cooperated with the course for nurses at the military hospital, creation of the Red Cross – Rio Grande do Norte branch –, volunteer course for boys, internship for official Army Reserve physicians and creation of the study center. Conclusion and implications for practice the study contributes to the understanding of care for women and the history of Rio Grande do Norte. From the idealization of the Maternity de Natal to the present day, it was possible to observe the evolution of its profile, initially providing assistance, and today it is a reference center for women’s and children’s health, education and professional training in health.
Saúde digital à luz da filosofia contemporânea
Authors: Yanes Carolina Young , Flôr Janaína da Silva , Amidianski Patrícia , Costa Rafaela Lira Mendes , Santos Evangelia Kotzias Atherino dos , Vargas Mara Ambrosina de Oliveira , Schoeller Soraia Dornelles
Published: August 2024

Resumo Objetivo discutir as questões relacionadas ao conceito de saúde digital, e de que modo a área da saúde tem se associado à evolução tecnológica e aos novos paradigmas para o cuidado, à luz de alguns pensadores da Filosofia contemporânea. Método trata-se de um ensaio teórico-filosófico. Resultados Michel Foucault destacou o papel das estruturas de poder na saúde digital, alertando para o potencial de vigilância e do controle social. Por outro lado, Pierre Lévy e Mário Bunge enfatizaram a importância da tecnologia na Enfermagem, ressaltando seus benefícios e desafios na busca por soluções adequadas e éticas. Considerações finais e implicações para a prática a saúde digital emerge como um terreno de conflitos e possibilidades, em que os avanços tecnológicos trazem consigo tanto as potencialidades transformadoras, quanto os riscos à privacidade, à autonomia e à qualidade do cuidado. A abordagem filosófica é essencial para questionar o desenvolvimento tecnológico em saúde, especialmente na Enfermagem, de maneira ética, profunda e consciente.
Cultural adaptation of the MISSCARE tool for use in the Chilean context
Authors: Saldia-Arteaga Lorena , Huaiquián-Silva Julia , Jara-Concha Patricia
Published: August 2024

Abstract Objective to validate the MISSCARE instrument for use in the Chilean context. Method sociolinguistic adaptation of MISSCARE involved translation and back-translation, followed by evaluation by English-speaking nurses. Finally, content validation was achieved through expert review, focusing on cultural and content adaptation. Results the 2.0 version of MISSCARE demonstrated both face and content validity. Missed care is an adverse event that threatens the safety and quality of health care. Having a validated instrument to measure missed care will serve as an indicator to improve the quality of health care provided. Conclusions and implications for practice the adapted version of MISSCARE is a reliable, valid tool for measuring missed nursing care in hospitals. The study of missed nursing care will generate essential knowledge to improve the quality of health care and to develop management indicators. These insights will drive strategies and interventions in order to optimize services and ensure a higher standard of care.
Adaptación cultural del instrumento MISSCARE para su uso en el contexto chileno
Authors: Saldia-Arteaga Lorena , Huaiquián-Silva Julia , Jara-Concha Patricia
Published: August 2024

Resumen Objetivo validar el instrumento de cuidado perdido de enfermería (MISSCARE), para ser utilizado en contexto chileno. Método adaptación sociolingüística de MISSCARE a través de traducción y retrotraducción, seguida por evaluación de profesionales de enfermería con manejo de idioma inglés y finalmente validación de contenido a través de revisión, adecuación cultural y de contenido, por expertos del área. Resultados el instrumento en su versión 2.0 demostró validez aparente y de contenido. El cuidado de enfermería perdido es un evento adverso que amenaza la seguridad y calidad de la atención en salud. Contar con un instrumento validado que mida el cuidado perdido será un indicador para mejorar la calidad en la atención prestada. Conclusiones e implicaciones para la práctica el instrumento MISSCARE en su versión adaptada es una herramienta confiable y válida para medir el cuidado perdido en instituciones hospitalarias. Estudiar los cuidados omitidos generará conocimientos clave para mejorar la calidad de la atención en salud y desarrollar indicadores de gestión. Estos conocimientos impulsarán estrategias e intervenciones para optimizar servicios y garantizar un estándar más alto en la atención.
Maturation of Erythrina speciosa Andrews seeds collected in the Southeast and South Regions of Brazil
Authors: Ribeiro Maiara Iadwizak , Rodrigues Guilherme de Almeida Garcia , Steiner Neusa , Bruxel Fernanda , Freitas Elisete Maria de , Barbedo Claudio José
Published: August 2024

ABSTRACT: Analyzing unripe phases of orthodox seeds and the variations in traits depending on environmental conditions under which they were formed is a way of gaining knowledge on the differences and similarities between orthodox and recalcitrant seed behavior. From this perspective, in this study fruits of Erythrina speciosa Andrews were collected in Florianópolis (SC), Lajeado (RS) and Sao Paulo (SP) in August and November. Seeds were extracted and classified into six different maturation stages. Seeds were stored at 7 °C for a maximum of five days until the beginning of the experiments. Moisture content and dry matter content were measured through the oven method (103 ± 3 °C). Germination was analyzed at 25 °C and 70% of relative humidity, with evaluations being made every two days for 30 days. To induce dormancy, seeds were dried to 10% moisture content. We ran an analysis of variance (ANOVA) and Tukey’s test at 5% as statistical analysis. We observed that unripe seeds showed a wide behavior variation depending on the collection period and site, for a same chronological age, reinforcing the idea that the seed behavior variation may be due to the maturation degree at the moment of seed dispersal.
A COMPREHENSIVE DEEP LEARNING ALGORITHM TO UNDERSTAND THE ROLE OF SOCIAL MEDIA IN CONSUMER PERCEPTION OF GREEN CONSUMPTION
Authors: Cerasi Ceren Cubukcu , Balcioğlu Yavuz Selim , Huseynov Farid , Kilic Asli
Published: August 2024

ABSTRACT This research proposes a comprehensive deep-learning algorithm to understand the role of social media in consumer perception of green consumption. After the COVID-19 pandemic, society has shown increased focus on the relationship between people and nature. Achieving sustainable development goals requires promoting green consumption, which necessitates understanding and influencing public attitudes toward sustainability. While previous studies have explored green consumption using behavioral models and surveys, they often overlook the perspective of social media. This study uses deep learning techniques to analyze social media data, including text and video content, to gain insights into consumer behavior and preferences. The study entails collecting data from X (former Twitter) and YouTube, developing deep learning algorithms for text classification, and creating a visualization and reporting system. More specifically, this study aims to analyze the impact of social media information sharing on society’s green purchasing intentions and proposes advanced architectures for text mining specifically the LDA method. This study highlights the valuable insights from analyzing social media discourse on green consumption. Trends, emotional attitudes, and engagement were examined using text mining and sentiment analysis. The study reveals platform-specific differences in sentiment and identifies influential keywords and phrases. The analysis also uncovers emotional responses and key factors associated with the discourse on green consumption. The findings can inform future strategies for promoting sustainable consumption. The study concludes by emphasizing the importance of further research to explore the discrepancies between platforms and harness the implications of these findings for sustainable consumption strategies.
El impacto psicosocial de la pandemia del covid-19 en las personas lesbianas, gais, bisexuales y transgénero (LGBT) en Colombia
Authors: Restrepo Pineda Jair Eduardo , Villegas Rojas Soraya , Ospina Ramírez Juan José
Published: August 2024

Resumen Este artículo presenta un análisis del impacto generado por la pandemia del covid-19 en los aspectos psicosociales de las personas lesbianas, gais, bisexuales y transgénero (LGBT) en Colombia. La metodología empleada fue mixta, con un enfoque secuencial explicativo, en el cual los resultados cualitativos se utilizaron para explicar los resultados cuantitativos y su énfasis fue interpretar las relaciones entre ambos; para tal fin se utilizaron la encuesta y los grupos de discusión como técnicas de investigación. En total, participaron en el estudio 219 personas LGBT, de las cuales 18 eran lesbianas, 128 gais, 36 bisexuales y 37 personas transgénero. Los impactos psicosociales más significativos asociados al covid-19 están relacionados con los aspectos económicos vinculados al desempleo y la disminución de los ingresos familiares, así como los conflictos en el ámbito familiar determinados por la pérdida de la privacidad de las personas LGBT que debieron regresar a sus hogares de origen; además de las tensiones en las relaciones sociales que generan sentimientos de inseguridad, temor y ansiedad en esta población debido al confinamiento obligatorio, lo cual evidencia una mayor desigualdad social vinculada a las orientaciones sexuales e identidades de género no hegemónicas que se acrecentaron en tiempos de pandemia.
Concepciones de la salud sexual y reproductiva en mujeres kurripacos en condición de movilidad humana
Authors: Fandiño Osorio Vilma Cenit , Velásquez Jiménez Claudia Marcela , Sarmiento Medina María Inés , Vargas Cruz Sandra Lucia , Puerto Miryam Beatriz
Published: August 2024

Resumen La migración de pueblos indígenas presenta tensiones entre las tradiciones ancestrales y la visión occidental, con implicaciones para la salud pública, particularmente en salud sexual y reproductiva. Este texto tuvo por objetivo analizar las concepciones sobre salud sexual y reproductiva de mujeres indígenas kurripacos de un resguardo del departamento de Guainía (Colombia). Se trata de un estudio de casos en una muestra de 40 mujeres indígenas kurripacos, residentes en el resguardo Paujil, en Guainía, procedentes de distintas comunidades. Se aplicaron entrevistas semiestructuradas construidas con miembros de la misma comunidad y traducidas a lengua nativa. Tres categorías emergieron del análisis: Impacto de la movilidad en las mujeres indígenas; autonomía relativa como marcos de la sexualidad; y concepciones frente al abordaje occidental de la salud sexual y reproductiva. Esta última categoría identificó que los aspectos de la salud sexual y reproductiva como constructo occidental son desconocidos por las participantes, quienes vislumbran formas propias de comprender la salud-enfermedad como parte integrada a todas las dimensiones de la vida/muerte. La interrelación entre las tradiciones ancestrales de los pueblos originarios y el abordaje occidental de la salud exige a profesionales, servicios y sistema un enfoque intercultural que reconozca la autonomía relativa personal y relacional.
Transposição do rio São Francisco: arte-fatos literários sobre saúde mental e o conto da implementação de megaempreendimentos no Brasil
Authors: Santos Giselle Oliveira , Santos Suely Emilia de Barros
Published: August 2024

Resumo A justificativa para a implementação de megaempreendimentos no Brasil perpassa discursos de interesse político, econômico, social e ambiental, a fim de promover o desenvolvimento do país. Neste levantamento, busca-se compreender como são contadas as histórias sobre a implementação dessas iniciativas em territórios onde povos camponeses habitam cotidianamente, bem como lançar um olhar para a interface entre megaempreendimentos e saúde mental, a fim de provocar reflexões que possibilitem a criação de ações interventivas de cuidado em saúde mental com os povos camponeses. Trata-se de uma revisão integrativa que consultou quatro bases de dados e analisou 36 escritos, que apontaram importantes considerações como o fato de a implementação de megaempreendimentos impactar diversos âmbitos da vida daqueles que residem no percurso das obras, ocasionando um processo de vulnerabilização desses povos. Quanto à saúde mental da população rural, sua compreensão na literatura ainda se focaliza na presença de transtornos. Ancorada no binômio saúde-doença, verifica-se a invisibilização da pluralidade de sentidos que a saúde mental tem para povos camponeses, bem como o desinteresse em conhecer os caminhos trilhados no próprio território para o encaminhamento do cuidado em saúde mental, através de seus saberes, cosmologias e tradições.
Arcabouço teórico-metodológico, funções e efeitos do apoio institucional à saúde no contexto do Sistema Único de Saúde
Authors: Magnago Carinne , França Tania , Belisário Soraya Almeida
Published: August 2024

Resumo Os arranjos de apoio institucional têm se destacado como um modo inovador de gestão. Este artigo objetivou sumarizar e analisar o arcabouço teórico-metodológico e os efeitos do apoio institucional no contexto do Sistema Único de Saúde (SUS) e sintetizar os conceitos, as funções e as habilidades do apoiador institucional. Uma revisão de escopo foi empreendida em diferentes bases científicas, resultando em 89 publicações, cuja análise indicou que os conceitos e métodos de apoio se ancoram no Método Paideia, que intenciona a democratização da gestão. Os apoiadores institucionais, com esse objetivo, atuam como articuladores dos diversos jeitos de gerir a micropolítica dos espaços coletivos, fornecendo suporte para o movimento de ampliação da descentralização do poder e para a geração de autonomia. Os efeitos de sua aplicação expressam processos de democratização institucional, que se traduzem como dilatação da autonomia dos sujeitos nos processos decisórios, da reflexão crítica e compreensão dos processos instituídos. Esses efeitos também expressam novos modos de produzir saúde, que buscam romper com as práticas de saúde repetitivas, cristalizadas e cronificadas. As evidências publicadas não nos permitiram refletir a respeito dos limites macro e microinstitucionais concretamente encontrados na implementação do apoio, colocando-nos o desafio de adentrarmos cientificamente nas práticas de socialização de recursos de poder operadas pela função apoio.
Reinventando a roda: Capoeira como dispositivo de cuidado em um Centro de Atenção Psicossocial Álcool e outras Drogas
Authors: Alves Stefania Vallado , Silva Carla Regina , Liberman Flavia
Published: August 2024

Resumo Este artigo apresenta alguns resultados de uma pesquisa de mestrado cujo objetivo foi identificar as repercussões da prática da capoeira como dispositivo de cuidado e promoção da saúde para pessoas que fazem uso prejudicial de substâncias psicoativas. A pesquisa seguiu as diretrizes do método cartográfico e acompanhou, no período de 2019 a 2020, as atividades de um grupo de capoeira que ocorriam semanalmente no Centro de Atenção Psicossocial - Álcool e Outras Drogas II (CAPS AD II), parte da Rede de Atenção Psicossocial na cidade de São Paulo. Os participantes eram usuários que se interessavam por essa prática. Foram produzidos diários de campo e registros fotográficos. A prática da capoeira, como experiência afrorreferenciada, é percebida como promotora de saúde, atuando como um dispositivo de cuidado que valoriza os espaços de encontros e trocas de saberes-fazeres-sentires, incluindo aspectos coletivos, sociais, políticos, econômicos e culturais, proporcionando resgate e significado singulares. A inserção da capoeira nos serviços de saúde torna-se um dispositivo importante para expressões e posicionamentos sociais, políticos e afetivos, configurando-se também como um espaço de aproximação de singularidades, por meio da mandinga e da malícia que compõem essa grande roda.
Capturing the random mechanical behaviour of granular materials: a comprehensive stochastic discrete-element method study
Authors: Liu DeYun , Lyu Meng-Ze
Published: August 2024

This research pioneers a stochastic discrete-element method (DEM) by integrating the probability density evolution method (PDEM), offering a novel approach to connect particle-scale property uncertainties, specifically inter-particle friction coefficient (μ) and particle shear modulus (Gp), with macro-scale soil behaviour. Through 1100 DEM simulations, this study reveals that, for uniform particle size distribution, the uncertainty in μ substantially affects large-strain soil behaviour, with its effect being associated with packing density and soil state. The uncertainty effect of μ remains pronounced at the critical state, while the packing density effect diminishes. Stress distribution appears insensitive to uncertainty of μ, rather suggesting a predominant influence of particle size distributions. In contrast, the uncertainty effect of μ becomes negligible on small-strain behaviour, demonstrating a limited effect on small-strain stiffness. Uncertainty in Gp presents limited effects on large-strain behaviour, including stress ratios and dilatancy. At small strains, Gp shows a significant impact on stiffness, diverging from minimal influence identified for μ. This study presents a framework that integrates experimental techniques to study particle-scale uncertainty propagation, enhancing predictions of macro-scale soil behaviour. This approach could be beneficial for precise multi-scale simulations, incorporating particle-level uncertainties in engineering-scale models, thereby improving geotechnical practice predictability.
Recent advances in vegetable oil based fine chemicals and polymers
Authors: Ma Yufeng , Hu Yun , Fang Yan , Li Qiaoguang , Huang Qin , Shang Qianqian , Zhang Meng , Li Shouhai , Jia Puyou , Zhou Yonghong
Published: August 2024

Vegetable oil as a kind of renewable resource has been considered as promising raw material to replace petrochemical resources. This paper focuses on several highly reactive sites of triglycerides, including carbon–carbon double bonds, allyl groups, and ester groups. The vegetable oil-based platform monomers/polymers with different structures and functions are obtained via chemical modification such as epoxidation, ozone decomposition, hydroformylation, transesterification, and thiolene. Vegetable oil-based chemicals mainly include polyols, biodiesel fuels, lubricants, and plasticizers, and vegetable oil-based polymers mainly include inks, coatings, and polyurethane foams. This paper summarizes and analyzes the review of vegetable oil-based chemicals and polymers in various industries, and puts forward the prospect for their future applications.
Auswirkungen neurologischer Erkrankungen auf die berufliche Leistungsfähigkeit
Authors:   Agenturverbund Hessen Gießen des Ärztlichen Dienstes, Agentur für Arbeit Wiesbaden, Fischer Philipp
Published: August 2024

Im folgenden Artikel sollen einige neurologische Fallkonstellationen aus dem Blickwinkel der Tätigkeit im Ärztlichen Dienst der Bundesagentur für Arbeit hinsichtlich der Integration in den Arbeitsmarkt beleuchtet und Empfehlungen zum weiteren Vorgehen ausgesprochen werden.
El sector de la construcción
Authors:   Universidad Politécnica Salesiana (Ecuador), Candelario Pin Ámbar , Ordóñez Arévalo Eddy ,   Universidad Politécnica Salesiana (Ecuador), Vera Santistevan Víctor ,   Universidad Politécnica Salesiana (Ecuador)
Published: August 2024

El estudio determinó la situación financiera de las pequeñas y medianas empresas del sector de la construcción, que desarrollaron actividades en el Ecuador frente a la pandemia provocada por la COVID-19. Se analizaron los datos del sector privado obtenidos desde los recursos que provee la Superintendencia de Compañías, los cuales son de la importancia para la economía ecuatoriana.
Comunicación de moda e inteligencia artificial: El caso de Neural Fashion AI
Authors: Díaz-Soloaga Paloma , Pelzer-Peinado Irene
Published: August 2024

Artificial intelligence is presented to society as a revolutionary tool capable of generating a change asunique as the democratization of Internet access at the beginning of the 21st Century. The different applications of AI are facilitating the development of marketing and communication strategies adapted to the needs of the public and the establishment of strong relationships with them. One of the most dynamic consumer markets is fashion communication, which is why we decided to delimit the applications of AI to brands in this sector. First goal was to identify the main resources and applications of AI that are being used to communicatewith the different stakeholders of fashion companies, particularly with the final consumer. Second objective was to recognize benefits and positive aspects along with the brakes and barriers that the application of this technology represents for the communication strategies of fashion brands. Thirdly, a case studyis offered to help academics and professionals understand how the fashion sector is receiving the helpof generative AI in the creation of campaigns. Through a combination of qualitative methods including3 Delphi interviews, a hemerographic research of professional publications and the Neural Fashion AI case study, the capacity of AI to point out a differentiating factor in the market that has to do withsustainability, product customization and optimization of company resources has been demonstrated. The main results highlight the contribution that AI makes to the efficiency of processes and to the achievement of brand objectives (customer satisfaction, loyalty, strengthening of positioning and brand image), expansion into new marketsand audiences, or the creation of innovative, impactful and attractive content.
Impacto de la inteligencia artificial en la moda. Análisis de influencers digitales en las fashion weeks internacionales
Authors: Fajardo Rodríguez-Borlado Paula , Pérez-Curiel Concha Pérez-Curiel
Published: August 2024

The phenomenon of digital influencers has transformed the way brands communicate with their audience via social networks. In a society that is governed by immediacy, companies have taken the lead in considering Instagram as the platform on which to advertise their products. In this scenario, influencers become a key tool and a business market for advertising. In parallel, Artificial Intelligence has given riseto a world of avatars, a non-human profile, that develop capabilities with unknown effects on audiencesand that focus the attention of fashion brands. The objective of this research is to understand how digital influencers communicate on Instagram and the impact on luxury brands. A dual-focus content analysis methodology (quantitative/qualitative) and SPSS statistical tool are applied. The first results show the level of frequency of digital influencers in the advertising campaigns of fashion brands, the presence of luxury brands in their conversations and a positive interaction with users, despite being profiles thatcannot reproduce the emotions of human language, we have been able to obtain some results whichsupport that, although the avatars generated by artificial intelligence (AI) have the necessary characteristics to play a relevant role in brand advertising on social networks, it cannot be said that are involved in the “influencer” profession in at least not in the near future. In the future, it may be equivalent to the work done by humans.
Kilómetro 0: inicio de las trayectorias migratorias de trabajadoras domésticas en Chile
Authors: Rodríguez-Covarrubias Natalie
Published: August 2024

This study aims to describe how the migratory trajectories of migrant domestic workers begin in Chile and the role of migratory networks and emotional factors in their decisions. A qualitative analysis of six biographical narratives is employed to examine the complexities of their labor and migratory trajectories, influenced by intersecting factors such as gender, class, and ethnicity, within the context of global care chains. The results reveal five central moments when making the decision to migrate. The first moment is characterized by the fact that none of the cases had worked as domestic workersin their home countries. In the second moment, the need to change their circumstances in their home countries arises, often due to political crises, economic challenges, or gender-based violence. The third moment involves the existence of a migratory network of friends and family who assist in their journey.In the fourth moment, a significant emotional factor plays a central role in the decision to migrate, influencing the timing of the decision. Finally, in the fifth moment, the decision to migrate to Chile is made.
UDA-Multiome-protocol v1
Authors: Li Yun
Published: August 2024

Droplet microfluidics-based single-cell combinatorial indexing sequencing represents an attractive way to balance cost, scalability, robustness, and accessibility. However, current methods need a tailored protocol for specific modality respectively, which may limit their potential for automation. We introduce UDA-seq, universal droplet microfluidics-based combinatorial indexing for massive-scale single-cell multimodal sequencing. We demonstrate that when necessary, UDA-seq enables effectively generating more than 100,000 single-cell data in a single-channel experiment of droplet microfluidics. Meanwhile, UDA-seq provides a universal workflow for accomplishing several multimodal tasks, including single-cell co-assay of RNA and VDJ, RNA and ATAC, and RNA and CRISPR guide RNA.
Protocol for use with NEBNext ULTRAEXPRESS® RNA Library Prep Kit (NEB#E3330) and NEBNext rRNA Depletion Kits (NEB #E7400, #E7405) and non indexed adaptor v1
Authors: Bonnevie Juliet
Published: August 2024

The NEBNext UltraExpress RNA Library Prep Kit contains the enzymes and buffers required to to rapidly convert 25–250 ng of total RNA into high-quality libraries for next-generation sequencing on the Illumina platform. The fast, simple workflow features minimal hands-on time and allows use of a single adaptor dilution and PCR cycling condition across the entire input range. In addition to the standard protocol, an appendix is included that details customized adaptor and cycling recommendations for varying RNA input amounts, if further optimization for library yields is required. If a stopping point is required between mRNA enrichment/ribosomal RNA depletion and library preparation, please refer to the FAQs tab for considerations and recommendations the FAQ tab is on NEB.com.
Percepción del clima ergonómico de la empresa y la presencia de molestias musculoesqueléticas en trabajadores
Authors:   Universidad Simón Bolivar, Mendinueta Martínez Martha Elena , Herazo Beltrán Aliz Yaneth ,   Universidad Simón Bolivar, Palacio Durán Erika Patricia ,   Universidad Simón Bolivar, Polo Gallardo Raúl Octavio ,   Universidad Simón Bolivar, Roa Bermúdez Eliana ,   Universidad Simón Bolivar, Casseres Fruto Miriam Dallana ,   Universidad Simón Bolivar
Published: August 2024

Objetivo: Estimar la relación entre la percepción del clima ergonómico de la empresa y la presencia de molestias musculoesqueléticas en trabajadores de tres ciudades colombianas. Metodología: Estudio de corte en 1339 trabajadores de empresas de diversos sectores económicos, ubicadas en Barranquilla, Bogotá y Cúcuta. El estudio se realizó desde septiembre hasta diciembre de 2021. Se aplicaron el Cuestionario Evaluación del Clima Ergonómico y el Cuestionario Nórdico Musculoesquelético para la evaluación de molestias musculoesqueléticas. Resultados: La mayoría de las molestias fueron en el cuello (48,88 %). El 48,69 % de los trabajadores encuestados considera que su formación y conocimiento sobre bienestar y salud en el ámbito laboral es baja. La percepción de molestias musculoesqueléticas en el cuello fue mayor en los trabajadores con baja percepción del compromiso de la Gerencia con los indicadores empresariales [OR 1,77 (IC 95 %: 1,24-2,51)] y con el bienestar y la salud [OR 2,56 (IC 95 %: 1,75-3,75)]. La baja formación y conocimiento de los empleados sobre los indicadores empresariales se relacionó con mayores molestias en hombros [OR 1,8 (IC 95 %: 1,26-2,58)] y codos [OR 2,3 (IC 95 %: 1,38-3,62)], y sobre el bienestar y la salud, con molestias en zona alta de la espalda [OR 3,7 (IC 95 %: 2,60-5,25)], cadera, nalgas, muslos [OR 3,19 (IC 95 %: 2,19-4,66)] y rodillas [OR 4,42 (IC 95 %: 3,02-6,46)]. Conclusión: La presencia de molestias musculoesqueléticas está relacionada con la falta de compromiso de la empresa con la gestión de un ambiente saludable, la participación de los trabajadores en las actividades de seguridad y salud en el trabajo, el análisis de riesgos laborales y los procesos de formación de los trabajadores.
Analisis Potensi Puisi “Indonesia, Tumpah Darahku” Karya Muhammad Yamin (1928) Sebagai Sumber Belajar Penguatan Profil Pelajar Pancasila
Authors: Khakim Moch Nurfahrul Lukmanul , Julian Royyan , Listyaputri Dinar Rizky , Yuliati Yuliati , Trisnadi Anif E.
Published: August 2024

Kajian ini bertujuan untuk menganalisis urgensi dan potensi puisi “Indonesia, Tumpah Darahku” sebagai sumber belajar penguatan profil pelajar Pancasila. Kajian ini menggunakan metode penelitian kualitatif dengan jenis deskriptif. Sumber data primer dalam kajian ini yaitu puisi karya Muhammad Yamin berjudul “Indonesia, Tumpah Darahku”, sedangkan data sekunder diperoleh dari berbagai literatur yang relevan. Teknik analisis data yang digunakan dalam kajian ini yaitu hermeneutik dengan membaca, menelaah, dan menafsirkan isi puisi secara langsung. Hasil kajian menunjukkan bahwa puisi berjudul “Indonesia, Tumpah Darahku” karya Muhammad Yamin Tahun 1928 memiliki pesan nasionalisme sehingga layak digunakan sebagai sumber belajar alternatif dalam penguatan profil pelajar Pancasila. Pemanfaatan puisi “Indonesia, Tumpah Darahku” dalam pembelajaran telah memenuhi dimensi profil pelajar Pancasila sebagai penerapan Kurikulum Merdeka. Guru dan peserta didik dapat menginternalisasikan enam dimensi profil pelajar Pancasila meliputi bertakwa kepada Tuhan Yang Maha Esa dan berakhlak mulia, berkebinekaan global, bergotong royong, mandiri, bernalar kritis, serta kreatif melalui puisi “Indonesia, Tumpah Darahku” dalam kehidupan sehari-hari.
Algorithm of surgical treatment for diaphyseal defects of the forearm bones due to gunshot injuries
Authors: Davydov D. V. , Brizhan L. K. , Kerimov A. A. , Maksimov A. A. , Khominets I. V. , Lychagin А. V. , Gritsyuk A. A. , Arsomakov А. Z.
Published: August 2024

Introduction In the current system of providing medical aid to wounded servicemen, along with the conservative primary surgical treatment and minimally invasive extrafocal fixation, high-tech surgical interventions of considerable complexity with the use of additive and tissue-engineering technologies have been coming to the forefront. It is necessary to determine their place in the current algorithm of limb bone defect management, which was the substantiation of our study.

The purpose of the study was to improve the algorithm for selecting a treatment method for patients with associated gunshot defects of the forearm based on the literature and clinical observations.

Materials and Methods We analyzed scientific articles in PubMed and Scientific Electronic Library (eLIBRARY.ru) platforms, published from 2004 to 2024, on the basis of which we could refine the algorithm of treatment method selection for patients with associated gunshot defects of the forearm. The developed algorithm was used to treat 178 patients with gunshot fractures of the forearm.

Results The review of the literature established the main provisions and principles that are applied in the reconstruction of the forearm with an associated defect. When choosing the method of bone defect management, a great number of authors tend to build a “reconstructive ladder”, moving from less severe (one bone) and extended defects (small defect up to 2 cm) to more complex (both bones) and massive defect (more than 10 cm). Upon having considered the revealed regularities, we improved the algorithm of surgical treatment of the latter, which is based on two classification principles: defect extention and location. Reconstruction of the forearm as a dynamic system after diaphyseal fractures requires consider the state of the radioulnar joint. The function of the latter depends on the length ratio of the radius and ulna bones. Therefore, we substantiated small (up to 2 cm) forearm bone defects that can be managed by simple surgical methods. Another fundamental addition to the algorithm was the allocation of a patients’ group with a defect of one forearm bone and a fracture of the other bone (defect-fracture); this combination allows avoiding complex surgical methods for reconstruction and use segment shortening.

Discussion The treatment of associated forearm defects is challenging, the choice of reconstruction technique remains uncertain, and the required consensus is lacking. Several forearm reconstruction techniques are available, yet there is no reliable evidence of their effectiveness in terms of treatment time, complications, reoperations, and functional recovery.

Conclusion The algorithm proposed for the treatment of extensive gunshot-associated defects of the forearm allows us to consider the change in the anatomy, make a surgical plan based on the reconstruction vector, and select optimal surgical techniques.
Effect of elastic intramedullary nailing on lower limb lengthening in acquired shortenings: a prospective study
Authors: Tropin D. V. , Tropin V. I. , Chertishchev A. A. , Gvozdev N. S. , Popkov D. A.
Published: August 2024

Introduction Lengthening and correction of limb deformities using Ilizarov external fixation is a frequent standard operation. However, the risk of complications associated with limb lengthening, including superficial or deep infection, contractures, secondary deformity, and fractures after device removal associated with delayed bone consolidation, remains significant.

The purpose of the work was to identify the features of bone lengthening with an external fixator in combination with elastic intramedullary nails, titanium or titanium with a composite hydroxyapatite coating, in the conditions of shortening of the lower extremities of acquired etiology.

Materials and methods The study included 64 patients, of which 31 patients underwent monofocal lengthening of the femur, 33 patients underwent monofocal lengthening of the tibia.

Results The mean external fixation indices (EFIs) of the groups compared for similar lengthening types (femoral or tibial lengthening) did not differ significantly for the types of intramedullary nails implanted. In femoral lengthening, a significant effect on the EFI had the nail type and the ratio of “nail diameter / medullary canal diameter”. The dependence of EFI on the nail type in tibial lengthening was associated with the ratio “nail diameter / internal diameter at the osteotomy site” (p = 0.023). Two-way ANOVA showed that the effect of the nail type on EFI depended on the nail diameter/ internal diameter at osteotomy site ratio in the tibial lengthening group (p = 0.034).

Discussion In acquired shortening of the lower extremities, there is no difference in EFI by using titanium elastic nails or intramedullary nails coated with composite hydroxyapatite. The use of a combined technique, in any case, has advantages: it provides good and excellent results without serious complications during lengthening in patients with shortening of acquired etiology. The strong positive correlation between the bone diameter/internal cortical distance ratio at the osteotomy site, coupled with the significant influence of the nail type and nail diameter on EFI, suggests that both factors should be considered together in future studies.

Conclusion In shortening of the lower extremities of acquired etiology, the use of a combined bone lengthening technique, comprising an external fixator in combination with elastic intramedullary nailing, provides good and excellent results without serious complications.
Fixation of the rotator cuff tendons for the greater tuberosity fracture of the humerus
Authors: Makovskiy A. A. , Shnayder L. S. , Popravka E. S.
Published: August 2024

Introduction Strategical approaches to managing patients with a greater tuberosity fracture of the humerus are controversial and there are discussions about surgical treatment options. Nondisplaced fractures require no surgical management. Shoulder replacement can be indicated for the surgical treatment of proximal humeral fractures with limited function of the upper limb and difficulties in refixation of the rotator cuff tendons due to the peculiar anatomical location of the tendon fixation. Solution to this problem can improve the quality of life of patients with greater tuberosity fracture of the humerus.

The objective was to evaluate the treatment outcome of a patient with a greater tuberosity fracture of the humerus repaired with open refixation of the rotator cuff tendons and medialization of the border of the articular surface of the humerus.

Material and methods A 46-year-old patient presented with limited movements and severe pain in the left shoulder after humerus dislocation and a greater tuberosity fracture.

Results The condition was repaired with an open repair of the rotator cuff tendons and medialization of the border of the articular surface of the humerus. The patient reported neither pain nor limited movements in the left shoulder at the one-year follow-up.

Discussion Treatment options for patients with a greater tuberosity injury remains controversial. The effectiveness and results of organ-saving surgery have not been explored and require scientific evaluation using a larger cohort of patients.

Conclusion Excellent functional results were achieved in a patient with injury to the greater tuberosity using surgical refixation of the rotator cuff tendons and medialization of the cartilaginous surface.
Multidisciplinary approach to repair of intra-articular fractures of the distal radius in a complicated setting (prehabilitation)
Authors: Khromov A. A. , Gumanenko E. K. , Linnik S. A. , Tkachenko A. N. , Kravtsov A. G. , Melnichuk A. V. , Lazutin A. S. , Zhulyabin A. V.
Published: August 2024

Introduction Treatment of malunited periarticular fracture of the distal metaepiphysis of the radius in a complicated setting is challenging and involved orthopaedic care and related specialties of neurologists, neurosurgeons and rehabilitation specialists. New methods are offered for repair of the distal radius fractures but the results obtained to date cannot be considered satisfactory, since the treatment is aimed at the restoration of the anatomical relationships and the hand function.

The objective was to demonstrate the role of prehabilitation preparing patients for elective reconstructive surgery, to present a multidisciplinary approach to the treatment of malunited radius fracture complicated by posttraumatic compression ischemic multineuropathy.

Material and methods The medical history of a 56-year-old patient with distal radius malunion complicated by posttraumatic compression ischemic multineuropathy was reviewed. Outcome criteria included absence of complaints and restored function of the hand and the wrist.

Results A positive functional outcome was recorded after prehabilitation and surgery. Early postop, the DASH scored 35, palmar flexion measured 64° with dorsiflexion of 61° and dynamometry of 30 kg seen with the left involved hand. A faster recovery of the hand function occurred due to regression of neurological disorders.

Discussion Treatment of the distal radius malunion in a complicated setting suggests the involvement of related specialists including neurologists, neurosurgeons, professionals in functional and diagnostic radiology, rehabilitation specialists so that the approach must be multidisciplinary. A preoperative course of prehabilitation supervised by a neurologist and a rehabilitation specialist is essential for the patient to achieve a higher basic level of functionality. Surgical treatment must be a stage of multi-stage multidisciplinary treatment of distal radius malunion in a complicated setting.

Conclusion The clinical case showed an effective multidisciplinary approach in the treatment of distal radius malunion in a complicated setting. Preoperative preparation (prehabilitation) had a positive effect on the postoperative recovery and functional results.
Use of Activated and Modified Pumice Stone for Removal of Mercury (II) and Arsenic (III) Ions From Aqueous Solution
Authors: Tirtom Vedia Nüket
Published: August 2024

Removal of Hg (II) and As (III) ions from aqueous solutions using activated and modified pumice stone were investigated. The pH, temperature, initial metal ion concentration which are very important for removal studies, were investigated by batch method. The experiments demonstrated that the equilibrium adsorption data ﬁt the Freundlich isotherm model well for Hg (II) and As (III) ions. The negative value of ΔH° = -199.92 kJ mol-1 and -78,15 kJ mol-1 for mercury (II) and arsenic (III) ions indicates that the adsorption process is exothermic. ΔS° were calculated as -267.85 J K-1 mol-1 for As (III) ions and the positive value of ΔS° = 0.69 kJ K-1 mol-1 for Hg (II) ions. The negative value of ΔG°=-405.14 kJ mol-1 for Hg (II) ions and -1.67 kJ mol-1 for As (III) ions indicates that adsorption is voluntary. EDTA has been found to be a good desorbent in desorption studies to recover arsenic and Hg ions. The experiments show that pumice stone can be used for Hg (II) and As (III) removal in aqueous solution.
MYSTICAL IMAGES IN THE ROMANTIC ERA ART WORKS: THROUGH ANCIENT LEGEND OF THE GISELLE BALLET TO PICTURESQUE CANVASES BY C.D. FRIEDRICH
Authors: Portnova Таtiana
Published: August 2024

The article is focused on a comparative analysis of the romanticism works by A. Adam and C.D. Friedrich.
The purpose of the study is primarily framed by the fact that it is the first attempt to jointly analyze the Giselle ballet and the paintings by C. Friedrich, and to provide their comprehensive description from a standpoint of mystical beauty reflected in Romanticism trend in art in the 19th century.
The author highlights the innovations originating in the ballet theatre and the pictorial art of the period, which landed a natural balance in the spatial organization principles between all components of performance and painting. It is shown that they cooperate at the junction of a figurative romantic idea as a powerful psychological tool.
DO GEOSSÍTIO FÍSICO AO VIRTUAL - CRIAÇÃO DE SITE PARA POTENCIALIZAÇÃO TURÍSTICO-PEDAGÓGICA NO LAJEDO MARINHO EM BOQUEIRÃO/PB
Authors: Rodrigues Golbery De Oliveira Chagas Aguiar , Albuquerque Danyllo Wagner , Das Chagas Jessé Oliveira , De Araújo Eduarda Sales , Pereira Dayan Rodrigues Gomes , De Freitas Miguel Ryan Dantas , Silva José Andrey Duarte , Cabral Pablo Matheus Porto
Published: August 2024

O distrito do Marinho, localizado no município de Boqueirão/PB, distante 45 km de Campina Grande, abriga um geossítio, cujas riquezas naturais/imateriais são imensas. O espaço contempla trilhas e apresenta atrativos, formas geográficas diversas (Pedra da Coxinha, do Jacaré, do Cachorro), cemitério indígena, pinturas rupestres, dentre outros. Faltava a organização de um espaço virtual que servisse concomitantemente de repositório e de marketing permanente do geoespaço em questão. Nesta perspectiva, a partir de parceria socioeducacional entre a associação/agentes locais e o IFPB em Campina Grande, através das ações de extensão e cultura, surgiu a ideia de criação de um site profissional, agregado com as redes sociais disponíveis, para potencializar ações do turismo em geral. Metodologicamente, na lógica de uma economia solidária, uma equipe de discentes, docentes e técnicos administrativos reuniu todo o material disponível e elaborou templates de sites e também um documentário para disseminação propagandística do espaço. Os resultados esperados contemplaram organização documental em espaço centralizado, maior visibilidade do geossítio e visitabilidade ao local, além de crescimento sustentável da economia subsidiária dos agentes atuantes no Lajedo do Marinho.Do ponto de vista teórico, embasamos a discussão em Araújo (2021) e Souza, Melo e Perinotto (2011).