bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of key biomarkers in the tubulointerstitium of patients with focal segmental glomerulosclerosis and their relationship with immune cell infiltration using weighted gene co-expression network analysis and least absolute shrinkage and selection operator
Authors: zhang Yun Xia , Bai Jun Yuan , Pu Xiao Wei , Lv Juan , Dai En Lai
Published: January 2023

Background
The pathogenesis of focal segmental glomerulosclerosis (FSGS) is unclear, and diagnostic methods are limited. This study aimed to identify key biomarkers in the tubulointerstitium of FSGS patients and their association with immune cell infiltration.
Methods
The microarray expression and related data( GSE108112 and GSE200818) were collected from the Gene Expression Omnibus database (https://www.ncbi.nlm.nih.gov/geo/). Identification and enrichment analysis of differentially expressed genes (DEGs) was performed. Additionally, PPI networks of the DEGs were constructed and classified using Cytoscape plug-in MCODE. Weighted gene co-expression network analysis was used to identify the most critical gene modules. Least Absolute Shrinkage and Selection Operator regression data were used to screen for key biomarkers of the tubulointerstitium in FSGS, and the receiver operating characteristic curve was used to determine their diagnostic accuracy. The major transcription factors affecting the hub genes were identified by Cytoscape plug-in iregulon. Infiltration of 28 immune cells and their interactions with hub genes were analyzed.
Results
In total, 535 DEGs were identified, including 219 upregulated genes and 316 downregulated genes. DEGs function mainly enriched in immune-related diseases and signaling fluxes. Cytoscape plug-in MCODE obtained nine modules with a total of 81 genes. The central module of WGCNA (green module, including 237 genes) in the correlation heap had the greatest association with the tubulointerstitial in FSGS. Three key genes (fractalkine/CX3C chemokine ligand 1 (CX3CL1), transforming growth factor beta 1 (TGFB1), and peroxisome proliferator activated receptor gamma coactivator 1 alpha (PPARGC1A)) were screened as potential tubulointerstitium biomarkers in FSGS. The transcription factor early growth response factor 1 (EGR1) had a regulatory effect on all three key biomarkers. Immune infiltration showed a significant correlation between CD4 + T cells, CD8 + T cells, and natural killer T cells. The results Infiltration of 28 immune cells showed that CX3CL1 and TGFB1 were enhanced, and PPARGC1A was decreased in immune and inflammation-related pathways.
Conclusions
The activation of natural killer T cells is closely related to tubulointerstitial renal lesions in FSGS. CX3CL1, TGFB1, and PPARGC1A may play important roles in the tubulointerstitium of FSGS through immune-related signaling pathways.
Performance of quantum registers in diamond in the presence of spin impurities
Authors: Maile Dominik , Ankerhold Joachim
Published: January 2023

The Nitrogen Vacancy Center in diamond coupled to addressable surrounding nuclear spins forms a versatile building block for future quantum technologies. While previous activities focused on sensing with only a single or very few spins in operation, recently multi-qubit registers have been successfully implemented for quantum information processing. Further progress requires a detailed understanding of the performance of quantum protocols for consecutive gate operations and thus, beyond established treatments for relaxation and dephasing.
Here, we provide such a theoretical analysis for a small spin registers with up to four spins built out of NV and environmental constituents in presence of ensembles of interacting impurity spins. Adapting a cluster correlation expansion, we predict coherence properties as well as fidelities for GHZ- and Bell-gate operations also in presence of decoupling pulses. The influence of the volume density and the geometry of the spin-bath consisting of substitutional nitrogen atoms are also taken into account.
Lernen mit Augmented Reality Technologie in der Hochschullehre
Authors: Furrer Florian , Bührer Wolfgang , Wyss Corinne , Degonda Adrian , Hiss Jan A.
Published: January 2023

Augmented Reality (AR) ist eine Technologie zur Anreicherung der Wahrnehmung der realen Umwelt mit virtuellen Elementen durch Geräte wie Smartphones, Tablets oder spezielle AR-Headsets wie die HoloLens. AR wird ein grosses Potenzial für den Einsatz in der Bildung zugesprochen. Die Erforschung ihres Einsatzes in der Bildung, insbesondere in Bezug auf die Lehrpersonenbildung, ist jedoch noch lückenhaft. Die Praktikabilität für den Einsatz von AR, der Einfluss auf die Lernprozesse und mögliche Herausforderungen, wie Usability oder Ablenkung, müssen vertieft untersucht werden. Da Lehrpersonen eine zentrale Rolle im Bildungsprozess einnehmen und deren Einstellungen für den Technikeinsatz entscheidend sind, wurde mit 18 angehenden Sekundarlehrpersonen an der Pädagogischen Hochschule Zürich eine Videostudie durchgeführt. Dazu wurde der Einsatz der Molekülbrowser-Software Molegram-Scientist auf dem AR-Headset HoloLens im Rahmen einer kollaborativen Lerneinheit explorativ erforscht. Die Auswertungen zeigen, dass trotz der Neuartigkeit der AR-Headsets und der daraus resultierenden potenziellen Herausforderungen die Lernaufgaben konzentriert gelöst wurden. Die Bedienbarkeit erwies sich als zufriedenstellend und der Umgang mit intuitiven Interaktionsmöglichkeiten, wie der Bewegung im Raum oder die Möglichkeiten zur Kollaboration, wurden intensiv genutzt. Übergreifend kann damit festgehalten werden, dass die AR-Technologie für ein kollaboratives Unterrichtssetting geeignet erscheint und weiter untersucht werden sollte.
Le NFT : de l'œuvre d'art à l'instrument financier
Authors: Bastian Enzo
Published: January 2023

Depuis quelques années, les Non-Fungible Tokens font l'objet d'un intérêt croissant notamment sur le marché de l'art. Ils suscitent des opérations spéculatives importantes, mais ne sont appréhendés juridiquement que de manière lacunaire. Ce manque d'encadrement présente des risques notamment sous l'angle du droit des marchés financiers et favorise des comportements criminels tels que le blanchiment d'argent. Dans l'attente d'une nécessaire modification législative, une solution pour réduire ces risques consisterait à catégoriser le NFT comme un instrument financier. Cet article aspire à illustrer comment une telle qualification serait envisageable ainsi que les conséquences y relatives.

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Das Interesse an Non-Fungible Token ist in den letzten Jahren insbesondere auf dem Kunstmarkt gestiegen. Sie führen zu beträchtlichen Spekulationsgeschäften, werden aber rechtlich nur lückenhaft erfasst. Dieser fehlende Rahmen birgt insbesondere unter dem Gesichtspunkt des Finanzmarktrechts Risiken und begünstigt kriminelle Verhaltensweisen wie Geldwäsche. Bis zu einer notwendigen Gesetzesänderung könnte eine Lösung zur Verringerung dieser Risiken darin bestehen, die NFT als Finanzinstrument zu kategorisieren. Dieser Artikel will aufzeigen, wie eine solche Einstufung aussehen könnte und welche Konsequenzen damit verbunden sind.
Obesity Genetics
Authors: Wicaksono Hendi
Published: January 2023

Obesity is a health problem that become burden all over the world, andÂ  affected all age groups. An increase in body weight above normal often characterises it. Genetic and environmental factors are known to be the main cause of obesity. Based on several studies, it turns out that genetic factors play a role of 40-60% in the incidence, however the role of genetic factors in obesity still not fully explained. The author wrote the topic of genetics and obesity based on his research experience regarding genotype polymorphisms associated with obesity. The aim of this book is to provide a new perspective on genetic factors as a causal factor and how genetics plays a role in obesity.
Lessons Learned from the Medical Education during the Covid-19 Pandemic for Future Training of Indonesian Doctors
Authors: Widagdo Maria Meiwati
Published: January 2023

Lessons learned from the experiences gained from the pandemic can benefit future medical education. Blended learning to educate more students without sacrificing quality may be an effective alternative.
Strategies for scientific popularization in the digital media environment
Authors:   St. Petersburg State University, Rysakova Polina I.
Published: January 2023

The proposed article is devoted to the analysis of the characteristic features of popular science communication in the digital media environment. The current models of scientific communication are analyzed, the key trends of modern society are identified — mediatization and digitalization, which set the specifics of scientific popularization today. The mediatized forms of science communication testify to the unfolding process of mediatization of science — the growing involvement of media communications in the implementation of scientific activities. The mediatization of science also has its own specifics, which, in particular, is manifested in the growing convergence of the spheres of science and politics. Such politicization of science sets the features of mediatization, which covers the field of scientific activity very unevenly. Those areas of scientific knowledge that are politically in demand are subjected to mediatization to a greater extent. The politicization of science, which is unfolding along with mediatization, makes it possible to identify a similarity between political and scientific communications. The digital media environment of science communications is largely built in a similar way with the space of political communications. It is characterized by polarization and differentiation of the audience. The acceptance of scientific communication is largely determined not by the quality of scientific content, but by the cognitive and value attitudes of users. The principal feature of digital media architecture is its dual nature, which allows it to be equally used both for the dissemination of scientific information and its refutation and approval of false information. Under these conditions, strategies for effective science communication may include measures aimed at increasing the media literacy of the audience, the flexible use of media framing of scientific knowledge, aimed at expanding the audience of science communication and overcoming prejudices regarding new scientific achievements.
Teaching press release as a hybrid genre in the Academic Writing course (English language, science majors)
Authors:   Moscow Institute of Physics and Technology, Alen’kina Tatiana B.
Published: January 2023

Science media discourse is dynamically developing in the 21st century, and the genre of a press release is gaining its popularity in the modern context of mediatization of science. Despite that, a press release remains to be underestimated both in media linguistics and in applied linguistics, English for specific purposes (ESP), and academic writing. The purpose of the article is to describe the process of teaching a press release within the framework of genre pedagogy. The first objective is to explore the hybrid nature of a press release by addressing the headlinesecondary headline-lead structure in English and Russian in the deconstruction stage. In the independent construction stage, the English-language press releases of Russian students are analyzed with a special emphasis on framing elements structure. The results showed the following trends. Students ignored secondary headlines, avoided hedges, and chose the favorite expressive means of the Russian tradition — alliteration and personification, thus demonstrating the influence of the Russian language and culture. In the process of writing leads, students used two major strategies. The first strategy was in telling a story of a research event and “forgetting” the scientific component. Another strategy was a different one — foregrounding the scientific component of the text. Students overexplained the topic and problem, making a lead similar to the Introduction of the research article. In both cases, hybridization of a press release seemed to be lost. The research has proven the fact of the significant role of a press release for the Academic Writing course. Undoubtedly, it is a good way to introduce the modern sociocultural context of mediatization of science as well as the variety of genres and their interdiscursive nature. In practice, however, the student writing demonstrates an influence of the Russian tradition in headlines and neglecting the hybrid nature of the genre in leads.
The WTO and issues of digital immersion of international trade
Authors: Habyyeva A. M.
Published: January 2023

The purpose and objectives of this article are determined by the pressing need for acceleration of the digitization dynamics in the international trade. The article emphasizes that in the modern time digital transformation is the key and biggest trend in the global trade business and its retail seсtor. The study has also provided a review related to the experiences of the top and leading global e-commerce countries. The features of the regulation of digital trade in the United States, China, India, and the European Union are identified and summarized. It is shown the World Trade Organization should play the key role in the process of unification and standardization of digital trade rules. The author provides recommendations on the key elements of a legally binding multilateral digital trade agreement and position of Turkmenistan in the context of accession to the World Trade Organization.
Conditions for the Borrowing of Irregular Plurals in an Intense Language Contact Situation
Authors: Stefan N. , Versloot A.
Published: January 2023

This article is devoted to borrowing in Frisian of Dutch irregular plurals formed by suffixation and stem vowel lengthening; e.g. dak [dak] ‘roof’ - daken [da:k?/da:kn?] ‘roofs’ instead of Frisian dak [dak] - dakken [dakn?]. Irregular plural formation is unproductive in Dutch and uncommon in Frisian (except in a couple of historical borrowings). Although the appearance of irregular Dutch plurals in Frisian had not previously been described or investigated, we hypothesize their existence in spoken Frisian, based on analogical cases. However, they would not be borrowed without any limitations as various factors would affect their borrowability; e.g. words frequency, phonotactic constraints or paradigm alternation. This study is based on approximately 175 interview recordings. The participants were asked to name different objects shown to them during the interview picture task. The results confirm both the (quite frequent) use of Dutch irregular plurals in Frisian and the involvement of different (morphological) factors in the borrowing processes. Accordingly, borrowing of such plurals is not only a lexical matter, but also a grammatical one. Phonotactic factors do not appear to play any significant role, but paradigm alternation seems to be decisive in whether a plural can successfully be borrowed or not.
Изменение адсорбционных свойств графена в процессе получения биосенсоров вирусных инфекций
Authors: Елисеев И.А. , Гущина Е.А. , Клотченко С.А. , Лебедев А.А. , Лебедева Н.М. , Лебедев С.П. , Нащекин А.В. , Петров В.Н. , Пузык М.В. , Роенков А.Д. , Смирнов А.Н. , Танклевская Е.М. , Усиков А.С. , Шабунина Е.И. , Шмидт Н.М.
Published: January 2023

The well-known effect of the local interaction between graphene and photoresist (LIGF) during the creation of biosensors is shown to lead to non-uniform distribution of compressive stresses, which deteriorates the adsorption properties of graphene, parameter reproducibility, and detecting ability of influenza B and SARS-Cov-2 biosensors. It is also shown that controlling the occurrence of LIGF areas on a graphene surface by atomic force microscopy or introducing a protective layer between graphene and photoresist can minimize the non-persistent effect of LIGF. The results of influenza B and SARS-CoV-2 imaging on the graphene surface in biosensor chips in a scanning electron microscope are presented.
Механизм протекания тока в композитной алюмохромной керамике при ее электронно-лучевом спекании в форвакууме
Authors: Зенин А.А. , Бакеев И.Ю. , Долгова А.В. , Климов А.С. , Окс Е.М.
Published: January 2023

It is shown that during electron beam sintering in the forevacuum pressure region of powder components of composite Al2O3-Cr ceramics, the magnitude of the current flowing through the sample depends both on the percentage of chromium and on the heating temperature of the sample. When a certain temperature is reached, the process of current flow is influenced by thermoelectric emission from the sample surface. Along with the ions from the beam plasma, a noticeable thermoelectric emission current contributes to the process of neutralizing the charging of the ceramic surface with an electron beam and reduces the absolute value of the negative potential of the sample surface. This ultimately contributes to an increase in the efficiency of energy transfer from the electron beam to the sample.
Уравнение состояния квантового кристалла
Authors: Рехвиашвили С.Ш. , Бухурова М.М. , Сокуров А.А.
Published: January 2023

A new and simple equation of state of a monatomic quantum crystal is constructed using the continuum approximation for interacting atoms and the Mie-Gruneisen theory. The equation makes it possible to describe the phase diagram both in the region of compressive pressures and in the vicinity of the critical point. Expressions for critical volume, pressure and temperature are found analytically. Comparison of numerical calculations with experimental data for helium shows satisfactory agreement.
Explaining Apparent deviations from Covered Interest Parity: Evidence from Mexico
Authors: Hernández Juan R.
Published: January 2023

This paper tests and quantifies the effects of reduced funding liquidity conditions on the covered interest parity (CIP) relating the U.S. Dollar-Mexican Peso market. To this end, a vector error-correction model is estimated. Results suggest, first, that apparent deviations from the CIP disappear when measures of funding liquidity for market participants are considered. Second, the exchange rate forward premium and the U.S. interest rate adjust towards the CIP cointegrating relationship. Finally, a structural analysis shows that deviations from CIP are mostly determined by shocks on the funding liquidity in the U.S. while funding liquidity conditions in Europe also have a non-negligible role. From the policy perspective, the paper underlies the relevance of funding liquidity measures when assessing whether the foreign exchange market works efficiently. As ever, there are some caveats in the analysis to consider. First, funding liquidity measures may shift from non- to stationary regimes. Second, market participants may not able to fund their liquidity at reference rates. The financial series present considerable ARCH-like behaviour, this may be a source of information to explore in further work.
Determinant of success factors in intelligent transport system (ITS) implementation in Jakarta
Authors: Nadhifa Khansa , Zulkarnain Zulkarnain
Published: January 2023

As the capital city of Indonesia, Jakarta has always become the cornerstone of economic development in this country. This condition then creates a logical consequence in which one of the crucial problems is transportation management implicating the congestion levels. This research is an initial attempt to design a structural framework of conceptual relationships among the determinants of the implementation of the intelligent transport system (ITS) in the land transportation system in Jakarta. The eight factors that affect the development of ITS strategy include funding, skills, operational guidance, human resources, utilization of available data, political policies, cooperation between parties, and socio-economic benefits. The Fuzzy-Total Interpretive Structural Modeling (TISM) method was applied to develop a structural framework that can provide greater flexibility to express the level of influence using fuzzy numbers. On the other hand, the Matrice d'Impacts Croisés Multiplication Appliquée un Classement (MICMAC) analysis method was used to improve the understanding and classification of factors based on the driving forces and interdependencies between factors. We found out that the skill was a driving factor that had the highest level of driving force compared to other factors and the success of ITS services in Jakarta can be judged from the socio-economic benefits because this factor was found to be determined by the sustainability of other factors.
Digital Phenotyping Data to Predict Symptom Improvement and Mental Health App Personalization in College Students: Prospective Validation of a Predictive Model
Authors: Currey Danielle , Torous John
Published: January 2023

Background

Mental health apps offer a transformative means to increase access to scalable evidence-based care for college students. Yet low rates of engagement currently preclude the effectiveness of these apps. One promising solution is to make these apps more responsive and personalized through digital phenotyping methods able to predict symptoms and offer tailored interventions.

Objective

Following our protocol and using the exact model shared in that paper, our primary aim in this study is to assess the prospective validity of mental health symptom prediction using the mindLAMP app through a replication study. We also explored secondary aims around app intervention personalization and correlations of engagement with the Technology Acceptance Model (TAM) and Digital Working Alliance Inventory scale in the context of automating the study.

Methods

The study was 28 days in duration and followed the published protocol, with participants collecting digital phenotyping data and being offered optional scheduled and algorithm-recommended app interventions. Study compensation was tied to the completion of weekly surveys and was not otherwise tied to engagement or use of the app.

Results

The data from 67 participants were used in this analysis. The area under the curve values for the symptom prediction model ranged from 0.58 for the UCLA Loneliness Scale to 0.71 for the Patient Health Questionnaire-9. Engagement with the scheduled app interventions was high, with a study mean of 73%, but few participants engaged with the optional recommended interventions. The perceived utility of the app in the TAM was higher (P=.01) among those completing at least one recommended intervention.

Conclusions

Our results suggest how digital phenotyping methods can be used to create generalizable models that may help create more personalized and engaging mental health apps. Automating studies is feasible, and our results suggest targets to increase engagement in future studies.

International Registered Report Identifier (IRRID)

RR2-10.2196/37954
Messenger Use and Video Calls as Correlates of Depressive and Anxiety Symptoms: Results From the Corona Health App Study of German Adults During the COVID-19 Pandemic (Preprint)
Authors: Edler Johanna-Sophie , Terhorst Yannik , Pryss Rüdiger , Baumeister Harald , Cohrdes Caroline
Published: January 2023

BACKGROUND

Specialized studies have shown that smartphone-based social interaction data are predictors of depressive and anxiety symptoms. Moreover, at times during the COVID-19 pandemic, social interaction took place primarily remotely. To appropriately test these objective data for their added value for epidemiological research during the pandemic, it is necessary to include established predictors.

OBJECTIVE

Using a comprehensive model, we investigated the extent to which smartphone-based social interaction data contribute to the prediction of depressive and anxiety symptoms, while also taking into account well-established predictors and relevant pandemic-specific factors.

METHODS

We developed the Corona Health App and obtained participation from 490 Android smartphone users who agreed to allow us to collect smartphone-based social interaction data between July 2020 and February 2021. Using a cross-sectional design, we automatically collected data concerning average app use in terms of the categories video calls and telephony, messenger use, social media use, and SMS text messaging use, as well as pandemic-specific predictors and sociodemographic covariates. We statistically predicted depressive and anxiety symptoms using elastic net regression. To exclude overfitting, we used 10-fold cross-validation.

RESULTS

The amount of variance explained (R2) was 0.61 for the prediction of depressive symptoms and 0.57 for the prediction of anxiety symptoms. Of the smartphone-based social interaction data included, only messenger use proved to be a significant negative predictor of depressive and anxiety symptoms. Video calls were negative predictors only for depressive symptoms, and SMS text messaging use was a negative predictor only for anxiety symptoms.

CONCLUSIONS

The results show the relevance of smartphone-based social interaction data in predicting depressive and anxiety symptoms. However, even taken together in the context of a comprehensive model with well-established predictors, the data only add a small amount of value.
Wave Regime of Vietnamese Waters on the Basis of Numerical Modeling and Field Measurements
Authors: Le Mau , Vlasova Galina , Lebedev Sergey , Nguyen Tuan , Ho The , Tran Binh , Nguyen Dung
Published: January 2023

This paper presents results of research of the wave regime in Vietnamese waters (South China Sea) based on the data of numerical modeling data using the WAM model (WAVE Modeling). The model domain covers the basin of the South China Sea (SCS). The bathymetry of the SCS used in the model is based on the ETOPO5 digital database. Wind parameters are based on the six-hour NCEP/NCAR reanalysis data with a resolution of ΔX = ΔY = 0.25° over the period 1979–2021. The field wave data measurements were collected by the Institute of Oceanography of Vietnam Academy of Science and Technology in the southern central Vietnamese waters in 2013. The statistical data of computed wave characteristics for the period of 43 years (1979–2021) illustrate that the main wave direction in Vietnamese waters was NE during the Northeastern (NE) monsoon, and in the opposite direction during the Southwestern (SW) monsoon. The NE monsoon wave was more dominant than that of the SW monsoon wave. Recurrence frequency (%) of significant wave height Hs >1.0 m (Hs – significant wave height is an average of 1/3 the largest of serial waves relative to average seawater level) greater than 50%

covered the northeastern, central region of the SCS, and central Vietnamese coast. The wave recurrence frequency in the Gulf of Tonkin and Gulf of Thailand was <40% and <30%, respectively. The central Vietnamese coast from Ly Son Island to Phu Quy Island was the strongest affected by wave action. The recurrence frequency of the maximum significant wave height Hs >3.5 m was greater than 1.5%. The Gulf of Tonkin (Bach Long Vi Island) and the Gulf of Thailand (Tho Chu Island) were less affected by

wave action than the central Vietnamese coast: the recurrence frequency of the maximum wave height (Hs>3.5 m) was less than 0.1%. Phu Quy and Con Dao Islands were more influenced by wave action during both seasons than the central coast of Vietnam.
Japanese Linguistic Politeness as Speakers’ Rational Choice and Social Strategy
Authors: Liu Xuexin
Published: January 2023

Japanese linguistic politeness is a commonly observed phenomenon and the speaker’s being linguistically polite is an expected social behavior in the Japanese society. Most previous studies of Japanese politeness describe such a polite social behavioral pattern at a superficial or observational level without exploring the linguistic nature of such a polite behavior or the speaker’s motivations for performing a polite speech act in a particular speech context. From some linguistic, sociolinguistic, psycholinguistic and sociological perspectives, this study defines “politeness” as the speaker’s rational choices and a social strategy in the Japanese culture beyond surface language forms themselves. This paper claims that so-called “polite” or “honorific” language forms as commonly employed by the speaker in various social interactions do not necessarily always indicate that such a speaker must be a polite person. The so-called “polite” language is “linguistic” in nature and is thus more about a particular language form itself than about the speaker himself/herself. This paper further claims that the speaker makes rational choices of particular polite language forms to realize his/her communicative intention with the outcomes as perceived. Thus, this study explores the relationship between polite language forms and their social, cultural, and pragmatic functions. It concludes that speakers in the same speech community are conscious of linguistic choices which conform to their normative views for the interaction types; there is no simple equation between polite forms and polite speakers, and speakers are rational actors in making linguistic choices.
EVALUATION OF ANTIOXIDANT, ANTIMICROBIAL, ANTICANCER, AND WOUND HEALING PROPERTIES OF LEAF EXTRACTS OF ACANTHUS ILICIFOLIUS L.
Authors: A. SHAMEELA KHADEEJA , JOHNEY JESTEENA , RAGUNATHAN R.
Published: January 2023

Objective: To investigate the in vitro antioxidant, antimicrobial, anticancer, and wound healing properties of leaf extracts of Acanthus ilicifolius.

Methods: Acanthus ilicifolius leaves were dried and extracts were prepared using different solvents using cold extraction method. The preliminary phytochemical analysis and quantitative methods were carried out using standard procedures. In vitro antioxidant activity was evaluated using DPPH assay. Disc diffusion method was employed to determine the antimicrobial activities of the extracts. The methanolic extract was subjected to FTIR and GC-MS analysis for characterization of phytochemicals. This extract was further subjected to HPLC analysis for characterization and quantification. The antiproliferative effects of methanolic extract was determined using MTT assay and wound healing properties were determined using Cell migration assay.

Results: The highest levels of phenol and flavonoids were detected in aqueous extracts, followed by chloroform. Methanolic extract showed the highest antioxidant activity as well as significant antibacterial activity against Klebsiella pneumoniae and Staphylococcus aureus. Squalene, a metabolite with known pharmaceutical applications, was identified in GC-MS, which was further confirmed and quantified using HPLC analysis. The methanolic extract showed cytotoxic activity against the cervical cancer cell line and showed a significant beneficial effect on wound healing in the L929 cell line. 

Conclusion: The results obtained in this study revealed that the leaf extracts of Acanthus ilicifolius possess potent antioxidant, antimicrobial, cytotoxic and wound-healing properties. These activities are due to bioactive components present in the extracts whose identification could lead to the development of new therapeutic agents.
NOVEL TOPICAL ANTI-AGING HERBAL COMPOSITION
Authors: MODI KARUNA , MODI KARUNA , SONI BHOOMI , TELI DIVYA , SHAH MAMTA
Published: January 2023

Objective: Anti-aging formulations are among the premier skin care products in the global market that are in huge demand. Different treatments are available to slow down skin aging, but are very expensive and are often found to produce adverse reactions to the skin in prolong use. Hence, present study has been designed to formulate poly herbal anti-aging skin care products and their evaluation thereon.

Methods: Anti-aging herbal skin care cream, gel and serum were prepared using two medicinal plants Hippophae salicifolia and Celosia argentea along with three bioactives caffeine, rutin and bakuchiol. The formulations were evaluated for physicochemical parameters like pH, texture analysis, acid value, short-term stability study, etc. Phenolics and flavanoid content were determined. Anti-aging potential was gauged by in silico studies using the glide tool of ‘Schrodinger’.

Results: Cream, gel and serum showed good physical appearance and were free from gritty particles and with smooth texture. Accelerated stability studies indicated insignificant changes in physicochemical parameters of the formulations. Based on the docking score and interaction with amino acid, compounds present in the plant extracts and bioactive showed good anti-aging activity.

Conclusion: The prepared herbal anti-aging formulations were found to be stable and exhibited good potential as an anti-aging combination. So, they can be used as an effective combination to protect skin from aging.
INTERNET ADDICTION AND ITS ASSOCIATION WITH ANXIETY IN MEDICAL UNDERGRADUATES–A CROSS-SECTIONAL STUDY
Authors: MANDLA MOUNIKA , GARUGU BALAJI , VUNNAM SHASHANKA , GOTTUMUKKULA VAIDYANATH
Published: January 2023

Objective: To study the prevalence of Internet Addiction among Medical undergraduates and Interns and exploration of its association with anxiety disorders.

Methods: It is a cross-sectional survey conducted among 540 undergraduate medical students and 93 Interns at Government Medical College, Ananthapuramu. Young’s Internet Addiction Test and Zung’s self-rated Anxiety scale were applied to measure Internet addiction and Anxiety, respectively.

Results: From the results, it was 36.3% of subjects had Internet Addiction, of which 2% had severe Internet Addiction. Anxiety prevalence was about 19.14 %, of which 16.8% had mild to moderate anxiety symptoms and 2.34% had severe anxiety. There was a significant association between Internet Addiction and Anxiety (p<0.0001). There is a negative correlation between the year of study and Internet Addiction (p<0.018).

Conclusion: Internet addiction was found among medical students and there was a significant association between Internet addiction and Anxiety among participants.
NUTRACEUTICALS: USING COMMON FOODS TO IMPROVE HEALTH, PREVENTING ILLNESS, AND ADDRESS SAFETY ISSUES
Authors: SHARMA JONNY KUMAR , SHARMA VIKAS , SHARMA DHEERAJ , ASIF MOHAMMAD
Published: January 2023

Important dietary components known as nutraceuticals have both therapeutic and nutritional impacts. The active ingredients in these foods, including such carotenoids, collagen hydrolysate, and dietary fibers, are what provide them their health benefits. Nutraceuticals have been shown to have a good impact on immunological and cardiovascular health, as well as play a part in the prevention of infections and cancer. Depending on its nature and manner of action, nutraceuticals can be divided into many types. Various nutraceutical categories and their therapeutic potential effects, including anti-cancer, antioxidant, anti-inflammatory, and anti-lipid activity in disease, will be examined in this study. In addition, the many ways in which these accepted methods and structures, their application, and human safety would be covered, along with recent trends and nutraceuticals’ potential for the development.
IN SILICO ANALYSIS AND DOCKING STUDY OF THE ACTIVE PHYTO COMPOUNDS OF MORINGA OLEIFERA AGAINST MARBURG VIRUS VP35 PROTEIN
Authors: CHAVAN SHIVAM , WANARASE SHUBHAM , SHARMA SAMEER
Published: January 2023

Objective: Marburg is a transmissible disease of the Filoviridae family. It infected a million people worldwide. Hence, an attempt was made to identify natural compounds from Moringa oleifera, having multiple medicinal values in Indian Ayurveda, to prevent the disease, using molecular docking, drug likeness prediction, absorption, distribution, metabolism, and excretion (ADME) analysis, and toxicity prediction.
Methods: Marburg main protein was retrieved from the protein data bank database. The ligands with poor binding and molecules that can affect docking were removed and docking is done with the PyRx tool. ADME and drug-likeness analysis were done using Swiss-ADME and absorption, distribution, metabolism, excretion, and toxicity (ADMET) lab web server.
Results: Ramachandran plot analysis shows the statistical distribution of the combinations of the backbone dihedral angles ϕ and ψ of the protein. Molecular docking studies show three compounds from M. oleifera have potential binding affinity to resist the main protein VP35 by preventing proteolytic cleavage, translation, and replication of the virus. ADMET profile and drug likeness and toxicity prediction showed that all three compounds Melanin, Diclazuril, and Tifentai were safe and possess drug-like properties.
Conclusion: The present study suggests that Melanin, Diclazuril, and Tifentai have significant binding affinity and they could inhibit the main protein VP35 and also helps to manage the therapeutic strategies against Marburg Virus.
Measurement and Analysis of the Stability of Local Fiscal Revenue
Authors: Fengze Yu
Published: January 2023

The stability of fiscal revenue, so called the fluctuation of fiscal revenue, refers to the fluctuation degree of local government's actual fiscal revenue deviating from the expected fiscal revenue. As the main way of funds for local governments to perform public service functions, fiscal revenue is an important starting point for local governments to regulate and participate in economic activities. The drastic fluctuation of fiscal revenue will interfere with the government's economic functions, reduce the quantity and quality of public services, and produce inefficient government activities. The economic and social activities carried out by governments at all levels in practice are numerous and complicated, which can be classified according to different purposes and perspectives. However, no matter which classification method is adopted, stable financial revenue is the core guarantee of government economic activities, which is in the position of "leading the development and affecting the whole body". Based on the combination variance method of white (1983), this paper constructs the stability index of local fiscal revenue, and measures the stability of fiscal revenue of all provinces in China, and interprets and analyzes the measurement results through the theoretical method of economics. It is found that there are significant regional differences in the fluctuation of local fiscal revenue in China. By comparing the changes of fiscal revenue fluctuation index in 2000, 2009 and 2018, the fluctuation index of fiscal revenue shows obvious regional differences. The fluctuation degree of the economically developed eastern coastal area is lower than that of the underdeveloped central and Western Region, and the southern region with lower economic activity is significantly lower than that of the north. On the other hand, the external shocks such as "replacing business tax with value-added tax" and financial crisis also have a positive impact on local tax fluctuations. Through the analysis of the experimental results, it is found that good economic foundation, capital accumulation, industrial structure and geographical location have a great impact on financial stability. Therefore, the government should pay attention to the gap between the stability of fiscal revenue in different regions, actively improve the economic foundation of the poor stability of the central and western regions, formulate differentiated economic and financial policies, vigorously develop the secondary and tertiary industries, and improve the stability of fiscal revenue to cope with regional economic risks and improve the administrative efficiency of the government.
Multimodalidad en tres tipos de textos de divulgación de la ciencia para niños
Authors: Camacho Javier , Vázquez Ahumada Maria Andrea
Published: January 2023

Utilizando la lingüística sistémico-funcional, se analizarán las configuraciones de construcción de significación de tres textos multimodales (Lemke, 2002; Painter, Martin, Unsworth, 2012; Ventola, Charles Kaltenbacher, 2004) con el fin de observar las estructuras discursivas de libros nformativos (Garralón, 2013) especializados en la divulgación de la ciencia para públicos infantiles. En particular, se observarán las características metafuncionales de los registros, entendidos como variaciones en las estructuras de construcción de significado que se presentan en el discurso y que son propias de un contexto de producción, así como las redes de registros que aparecen en el género de la divulgación científica (Bhatia, 2014, 2017; Martin Rose, 2008; Matthiessen, 2015), con el objetivo de construir conocimiento especializado. La aproximación desde la perspectiva metafuncional posibilita el análisis de las estructuras significativas base sobre las que se construyen los textos, y sus reverberaciones en el plano de la construcción de la experiencia en función del contexto en que se presenta.
Meropenem Incorporated ZnO Nanoflakes as Nano Antibiotics: Efficient Antimicrobial Activity against Metallo β-lactamase Producing Clinical Isolates
Authors: Singh Brij Nandan , Patil Praful S. , Shah Harshada , Ashfaq Mohammad , Singh Ankit , Upadhyay G.C.
Published: January 2023

The number of fatalities caused by multidrug-resistant (MDR) bacteria is over 700,000 annually due to widespread antibiotic usage. So, there is a need of new antibiotics, materials that work like antibiotics, or combinations of antibiotics with nanomaterials that could help in treating the infections which is caused by MDR bacteria. The present study describes the synthesis of ZnO nanoflakes using a co-precipitation method. The ZnO nanoflakes and ZnO nanoflakes combinations with carbapenem antibiotics were tested against carbapenem-resistant (CR) clinical isolates. The SEM analysis showed surface morphology of the synthesized nanoflakes-like structure of ZnO. All 67 CR isolates were tested and showed inhibitory action at varying concentrations of ZnO nanoflakes. ZnO nanoflakes were found to have an inhibitory effect against Escherichia coli and Klebsiella pneumoniae at lowest concentration of 1.25 mg.ml-1 of ZnO NPs with average zone size (mean ±SD) 1.91±2.94 mm and 2.00±4.14 mm and the average zone size of ZnO nanoflakes against Acinetobacter baumanni and Pseudomonas aeruginosa was 9.89±0.76 mm and 10.17±0.39 mm at 2.5 mg.ml-1 concentration. The combined action of ZnO nanoflakes with Meropenem 10 mcg demonstrated synergetic activity against CR pathogens, with an average zone of inhibition measuring 15.2 mm in diameter. ZnO nanoflakes illustrated considerable antibacterial activity against MBL-producing gram-negative clinical isolates at the lowest concentration. Chemically synthesized ZnO nanoflakes may offer a superior future expectation as a nano-antibiotic to treat the infection caused by CRE bacteria.
Assessment of ESP students’ learning outcomes in a digital learning environment
Authors: Inozemtseva Kira M. , Morozova Elizaveta V. , Kolesnikov Ilya M.
Published: January 2023

Problem statement. The description of a pedagogic research in the field of formative assessment theory and practice is presented. The goal of the study is to define pedagogic and methodological conditions for effective evaluation of ESP students’ learning outcomes in a digital learning environment. Methodology. On the basis of key theoretical provisions of formative assessment and interdisciplinary approaches LSPCLIL, a complete set of criteria-based ESP evaluation tasks and assessment schemes were developed for Business English course delivered in the groups of BMSTU masters students majoring in economics. The approach to grading that correlates with BMSTU point-rating system was substantiated. The comparative analysis of modern platforms allowed to select the domestic service pruff.me, which was used as a ground for a digital assessment space (DAS), a part of BMSTU digital learning environment needed for implementing formative assessment of ESP learning outcomes. Results. The developed evaluation materials integrated in the DAS were tested by ESP students in a distant format. Conclusion. The research showed that creating DAS as an essential component of a university digital learning environment can contribute to effective assessment of ESP students’ competence-based learning outcomes provided that underlying methodology is taken into account and formative assessment technology is implemented in full volume.
Using mobile 3D models for demonstration unaccessible laboratory experiments and objects during study chemistry and biology in basic school
Authors: Balkina Ekaterina A.
Published: January 2023

Problem statement. The technological development of augmented reality is becoming more impressive every year. But, despite all the advantages and possibilities of using augmented reality in the modern world, it is still in its infancy in the field of education. One of the biggest benefits of using augmented reality technology in natural sciences is its ability to visualize abstract concepts, reproduce expensive, dangerous or time-consuming experiments, and showcase rare objects. These augmented reality abilities are great for teaching chemistry and biology in basic school, as they can visualize inaccessible objects, processes and phenomena in the classroom without using large time, material and technical resources. At the moment, sufficient experience has not been accumulated in the application of this technology in the study of natural sciences, in particular chemistry and biology. Thus, there is a need to conduct an appropriate scientific and pedagogical research to determine the effect of the introduction of augmented reality technology in the study of natural sciences. Methodology. The methods of designing a learning model, pedagogical experiment, observation, conversation, and generalization of the experience gained were used. Results. The results of a pedagogical experiment on the use of mobile 3D models to demonstrate inaccessible laboratory experiments and objects in the study of chemistry and biology in basic school, aimed at analyzing and highlighting the most significant examples of the application of this technology in practice. The essence and features of the use of mobile 3D models in the study of chemistry and biology are described. Significant aspects of the use of such technology in the modern school are revealed. Conclusion. The experience of organizing lessons with a demonstration of inaccessible laboratory experiments and objects in the study of chemistry and biology in a basic school allows us to conclude that there are significant differences in the educational process implemented using mobile 3D models and augmented reality technology.
Praktis dan Polemik Berhubung Taqbil dalam Kalangan Masyarakat Muslim di Tanah Melayu Menjelang Perang Dunia Kedua
Authors: Lee Nazirah
Published: January 2023

Taqbil, a customary practise in the Malay States, is exercise by non-saiyyids (Malay and Pro-Irshadi) to kiss a saiyyid's hand in order to show respect or obtain the latter's blessing. The chain of events that unfolded in the 1930s until 1941 altered Muslims' perceptions regarding taqbil. This paper attempts to analyze the debate surrounding the practise of taqbil in the period under study. Some began to question the practise of taqbil on the basis of equality and social status principles, claiming that taqbil is incompatible with Islamic teaching. Thus, the discussion also illustrates criticism raised by non-saiyyids especially the Malays against the saiyyid Alawiyyin. Arguments presented in this paper are derived from secondary and primary sources such as files, documents, and newspapers published within the period of study. Those were analysed based on historical research methodology. Findings suggest that the conflict and bickering regarding the practise of taqbil intensified towards the end of the 1930s and the early 1940s. In conclusion, on the eve of the Second World War, Muslims were divided into groups based on their perceptions toward the practise of taqbil and those involved refuse to budge even an inch citing religion or nationalism as the biggest reason for their dogged determination.