bluuebunny/crossref_metadata_2025 huggingface dataset:
Impairment of Mesenteric Perfusion as a Marker of Major Bleeding in Trauma Patients
Authors: Jávor Péter , Donka Tibor , Horváth Tamara , Sándor Lilla , Török László , Szabó Andrea , Hartmann Petra
Published: May 2023

The majority of potentially preventable mortality in trauma patients is related to bleeding; therefore, early recognition and effective treatment of hemorrhagic shock impose a cardinal challenge for trauma teams worldwide. The reduction in mesenteric perfusion (MP) is among the first compensatory responses to blood loss; however, there is no adequate tool for splanchnic hemodynamic monitoring in emergency patient care. In this narrative review, (i) methods based on flowmetry, CT imaging, video microscopy (VM), measurement of laboratory markers, spectroscopy, and tissue capnometry were critically analyzed with respect to their accessibility, and applicability, sensitivity, and specificity. (ii) Then, we demonstrated that derangement of MP is a promising diagnostic indicator of blood loss. (iii) Finally, we discussed a new diagnostic method for the evaluation of hemorrhage based on exhaled methane (CH4) measurement. Conclusions: Monitoring the MP is a feasible option for the evaluation of blood loss. There are a wide range of experimentally used methodologies; however, due to their practical limitations, only a fraction of them could be integrated into routine emergency trauma care. According to our comprehensive review, breath analysis, including exhaled CH4 measurement, would provide the possibility for continuous, non-invasive monitoring of blood loss.
Descemet Membrane Endothelial Keratoplasty (DMEK) Reduces the Corneal Epithelial Thickness in Fuchs’ Patients
Authors: Storp Jens Julian , Lahme Larissa , Al-Nawaiseh Sami , Eter Nicole , Alnawaiseh Maged
Published: May 2023

Fuchs’ endothelial corneal dystrophy (FECD) is the occurrence of corneal edema due to endothelial cell dystrophy. Descemet membrane endothelial keratoplasty (DMEK) is considered to be the gold standard of treatment. The aim of this study was to investigate the changes in the corneal epithelial thickness of FECD patients before and after DMEK and to compare these results with a healthy control cohort. In this retrospective analysis, 38 eyes of patients with FECD that were treated with DMEK and 35 healthy control eyes received anterior segment optical coherence tomography (OCT; Optovue, XR-Avanti, Fremont, CA, USA). The corneal epithelial thicknesses in different locations were analyzed and compared between the preoperative, postoperative, and control cohorts. The median follow-up time was 9 months. There was a significant degression of the mean epithelial thickness after DMEK in the central, paracentral, and mid-peripheral zones (p < 0.01) of the cornea. The total corneal thickness and stromal thickness decreased significantly as well. No significant differences were observed between the postoperative and control cohorts. In conclusion, the FECD patients had an increased epithelial thickness compared to the healthy controls, which decreased significantly after DMEK and reached thickness levels comparable to those of healthy control eyes. This study emphasized the importance of distinguishing between the corneal layers in anterior segment pathologies and surgical procedures. Moreover, it accentuated the fact that the structural alterations in FECD extend beyond the corneal stroma.
High Risk for Persistent Peri-Prosthetic Infection and Amputation in Mega-Prosthesis Reconstruction
Authors: Berger Christina , Parai Catharina , Tillander Jonatan , Bergh Peter , Wennergren David , Brisby Helena
Published: May 2023

A peri-prosthetic joint infection is a feared complication after mega-prosthesis reconstruction of large bone defects. The current study investigates how patients operated with a mega-prosthesis due to sarcoma, metastasis, or trauma, are affected by a deep infection focusing on re-operations, risk for persistent infection, arthrodesis, or subsequent amputation. Time to infection, causative bacterial strains, mode of treatment and length of hospital stay are also reported. A total of 114 patients with 116 prostheses were evaluated, a median of 7.6 years (range 3.8–13.7) after surgery, of which 35 (30%) were re-operated due to a peri-prosthetic infection. Of the infected patients, the prosthesis was still in place in 51%, 37% were amputated, and 9% had an arthrodesis. The infection was persistent in 26% of the infected patients at follow-up. The mean total length of hospital stay was 68 (median 60) days and the mean number of reoperations was 8.9 (median 6.0). The mean length of antibiotic treatment was 340 days (median 183). Coagulase-negative staphylococci and Staphylococcus aureus were the most frequent bacterial agents isolated in deep cultures. No MRSA- or ESBL-producing Enterobacterales were found but vancomycin-resistant Enterococcus faecium was isolated in one patient. In summary, there is a high risk for peri-prosthetic infection in mega-prostheses, resulting in persistent infection or amputation relatively often.
Edge-to-Edge Transcatheter Mitral Valve Repair Using PASCAL vs. MitraClip: A Systematic Review and Meta-Analysis
Authors: Hosseini Kaveh , Soleimani Hamidreza , Nasrollahizadeh Amir , Jenab Yaser , Karlas Angelos , Avgerinos Dimitrios V. , Briasoulis Alexandros , Kuno Toshiki , Doulamis Ilias , Kampaktsis Polydoros N.
Published: May 2023

Background: Transcatheter edge-to-edge repair (TEER) of the mitral valve (MV) can be performed using the PASCAL or MitraClip devices. Few studies offer a head-to-head outcome comparison of these two devices. Material and Methods: PubMed, EMBASE, Cochrane Library, Clinicaltrials.gov and WHO’s International Clinical Trials Registry Platform, from 1 January 2000 until 1 March 2023, were searched. Study protocol details were registered in the International Prospective Register of Systematic Reviews (PROSPERO ID: CRD42023405400). Randomized Controlled Trials and observational studies reporting head-to-head clinical comparison of PASCAL and MitraClip devices were eligible for selection. Patients with severe functional or degenerative mitral regurgitation (MR) who had undergone TEER of the MV with either PASCAL or MitraClip devices were included in the meta-analysis. Data from six studies (five observational and one randomized clinical trial) were extracted and analyzed. The main outcomes were a reduction in MR to 2+ or less, improvement of New York Heart Association (NYHA) and 30-day all-cause mortality. Peri-procedural mortality, success rate and adverse events were also compared. Results: Data from 785 and 796 patients that underwent TEER using PASCAL and MitraClip, respectively, were analyzed. Thirty-day all-cause mortality (Risk ratio [RR] = 1.51, 95% CI 0.79–2.89), MR reduction to maximum 2+ (RR = 1.00, 95% CI 0.98–1.02) and NYHA improvement (RR = 0.98, 95% CI 0.84–1.15) were similar in both device groups. Both devices had high and similar success rates (96.9% and 96.7% for the PASCAL and MitraClip group, respectively, p value = 0.91). MR reduction to 1+ or less at discharge was similar in both device groups (RR = 1.06, 95% CI 0.95–1.19). Composite peri-procedural and in-hospital mortality was 0.64% and 1.66% in the PASCAL and MitraClip groups, respectively (p value = 0.094). Rates of peri-procedural cerebrovascular accidents were 0.26% in PASCAL and 1.01% in MitraClip (p value = 0.108). Conclusions: Both PASCAL and MitraClip devices have high success and low complication rates for TEER of the MV. PASCAL was not inferior to MitraClip in reducing the MR level at discharge.
Comparative Study of Anticipatory Postural Adjustments between Normal and Cervical Myelopathy Patients
Authors: Funao Haruki , Igawa Tatsuya , Matsuzawa Masaru , Isogai Norihiro , Ishii Ken
Published: May 2023

Patients with cervical spondylotic myelopathy or ossification of the posterior longitudinal ligament have been considered to be prone to falls due to lower extremity dysfunction and gait instability. Anticipatory postural adjustments (APAs) are unconscious muscular activities to counterbalance perturbation. To date, there are no reports on APAs in cervical myelopathy patients, and quantification of postural control remains difficult. Thirty participants were enrolled, of which 15 were cervical myelopathy patients and 15 were normal age- and sex-matched controls. A three-dimensional motion capture system with force plates was used, and the APA phase was defined as the time between start of movement at the center of pressure and heel-off of the step leg. The APA phase (0.47 vs. 0.39 s, p < 0.05) and turning time (2.27 vs. 1.83 s, p < 0.01) were significantly longer, whereas step length tended to be shorter (305.18 vs. 361.04 mm, p = 0.06) in cervical myelopathy patients. There was a significant correlation between Japanese Orthopaedic Association lower extremity motor dysfunction scores and step length (p < 0.01). Cervical myelopathy patients are prone to falls due to longer APA phases with shorter step lengths. Analysis of the APA phase aids the visualization and quantification of postural control during initial gait in cervical myelopathy patients.
A Global Review of Publicly Available Datasets Containing Fundus Images: Characteristics, Barriers to Access, Usability, and Generalizability
Authors: Krzywicki Tomasz , Brona Piotr , Zbrzezny Agnieszka M. , Grzybowski Andrzej E.
Published: May 2023

This article provides a comprehensive and up-to-date overview of the repositories that contain color fundus images. We analyzed them regarding availability and legality, presented the datasets’ characteristics, and identified labeled and unlabeled image sets. This study aimed to complete all publicly available color fundus image datasets to create a central catalog of available color fundus image datasets.
Comparison of Two Types of Guidewires for Malignant Hilar Biliary Obstruction by Endoscopic Retrograde Cholangiopancreatography: A Randomized Controlled Trial
Authors: Han Sung Yong , Choe Jung Wan , Kim Dong Uk , Hyun Jong Jin , Han Joung-Ho , So Hoonsub , Bang Sung Jo , Koh Dong Hee , Jeong Seok
Published: May 2023

Background: There is insufficient information regarding the optimal guidewire for managing malignant hilar biliary obstruction (MHBO). Therefore, a newly designed 0.025-inch guidewire was compared with the conventional 0.035-inch guidewire for selective cannulation of both intrahepatic ducts (IHDs) in patients with MHBO. Methods: Patients were randomly enrolled into the curved type newly designed 0.025-inch guidewire group (0.025 group) or the curved type conventional 0.035-inch guidewire group (0.035 group). The primary outcome was the selective cannulation rate of IHD. If the assigned guidewire failed to pass the stricture within 5 min, the crossover guidewire was selected. If the crossover guidewire failed to cross the stricture within the next 5 min, it was judged as a failed selective cannulation of both IHDs. Results: A total of 90 patients were enrolled (0.025 group, n = 47; 0.035 group, n = 43). There was no significant difference in baseline characteristics between the groups regarding sex, age, BMI, obstruction level, and clinical presentation. Four patients (8.5%) in the 0.025 group the cannulation of the IHD failed and the conventional 0.035-inch guidewire was substituted in a second attempt; the 0.035-inch guidewire failed to cross the stricture in all four patients. In the 0.035 group, eleven patients (25.6%) failed to achieve selective cannulation of IHD, and the 0.025-inch guidewire was substituted; the newly designed 0.025-inch guidewire crossed the stricture in ten of these (10/11, 90.9%). The selective cannulation rate of IHD was significantly higher in the 0.025 group (95.1% vs. 85.5%, p = 0.043). Conclusions: The 0.025 group exhibited a higher success rate for selective cannulation of both IHDs in MHBO than did the 0.035 group.
The Effectiveness of Pyruvic Acid Peeling in Improving the Quality of Life of Patients with Acne Vulgaris
Authors: Jankowska Beata , Zujko Małgorzata Elżbieta
Published: May 2023

Improving the quality of life (QoL) of patients with various chronic diseases has become a challenge and priority of contemporary medicine. The aim of this study was to assess the impact of pyruvic acid peeling on the QoL of patients with acne vulgaris. The study group consisted of 200 young patients (mean age 23.04 ± 4.71) with acne vulgaris of mainly mild or moderate severity. Basic data about the patient were collected using a self-reported questionnaire. The assessment of QoL was carried out using standardized questionnaires: CADI (Cardiff Acne Disability Index), DLQI (Dermatology Life Quality Index), SWLS (Satisfaction With Life Scale), and BDI (Beck Depression Inventory). The cosmetic intervention consisted of chemical peeling with 35% pyruvic acid for acne lesions on the body and included four series repeated at 7-day intervals. This study demonstrated that acne vulgaris impairs the quality of life of young people. There were no significant differences between the severity of acne and the lifestyles of the subjects. The applied cosmetic procedure significantly decreased the severity of the acne and improved the quality of life of the patients.
Attenuation of Dopaminergic Neurodegeneration in a C. elegans Parkinson’s Model through Regulation of Xanthine Dehydrogenase (XDH-1) Expression by the RNA Editase, ADR-2
Authors: Starr Lindsey A. , McKay Luke E. , Peter Kylie N. , Seyfarth Lena M. , Berkowitz Laura A. , Caldwell Kim A. , Caldwell Guy A.
Published: May 2023

Differential RNA editing by adenosine deaminases that act on RNA (ADARs) has been implicated in several neurological disorders, including Parkinson’s disease (PD). Here, we report results of a RNAi screen of genes differentially regulated in adr-2 mutants, normally encoding the only catalytically active ADAR in Caenorhabditis elegans, ADR-2. Subsequent analysis of candidate genes that alter the misfolding of human α-synuclein (α-syn) and dopaminergic neurodegeneration, two PD pathologies, reveal that reduced expression of xdh-1, the ortholog of human xanthine dehydrogenase (XDH), is protective against α-synuclein-induced dopaminergic neurodegeneration. Further, RNAi experiments show that WHT-2, the worm ortholog of the human ABCG2 transporter and a predicted interactor of XDH-1, is the rate-limiting factor in the ADR-2, XDH-1, WHT-2 system for dopaminergic neuroprotection. In silico structural modeling of WHT-2 indicates that the editing of one nucleotide in the wht-2 mRNA leads to the substitution of threonine with alanine at residue 124 in the WHT-2 protein, changing hydrogen bonds in this region. Thus, we propose a model where wht-2 is edited by ADR-2, which promotes optimal export of uric acid, a known substrate of WHT-2 and a product of XDH-1 activity. In the absence of editing, uric acid export is limited, provoking a reduction in xdh-1 transcription to limit uric acid production and maintain cellular homeostasis. As a result, elevation of uric acid is protective against dopaminergic neuronal cell death. In turn, increased levels of uric acid are associated with a decrease in ROS production. Further, downregulation of xdh-1 is protective against PD pathologies because decreased levels of XDH-1 correlate to a concomitant reduction in xanthine oxidase (XO), the form of the protein whose by-product is superoxide anion. These data indicate that modifying specific targets of RNA editing may represent a promising therapeutic strategy for PD.
Anti-Inflammatory Activity of Mycobiont Extract of Parmotrema austrosinense (Zahlbr.) Hale in a Zebrafish Model
Authors: Rajendran Kalidoss , Karuppiah Ponmurugan , Ponnusamy Ponmurugan , Shaik Mohammed Rafi , Khan Mujeeb , Oh Tae Hwan , Shaik Baji
Published: May 2023

Thousands of different kinds of lichen metabolites are being examined for their biological activities, including anticancer properties. In this context, the present study aims to assess the anti-inflammatory activity of the acetone extract of Parmotrema austrosinense mycobiont. A lipid peroxidation assay was performed with the acetone extracts of P. austrosinense mycobiont, which was further used to evaluate its anti-inflammatory efficacy using a zebrafish model. Furthermore, the histopathological study was also carried out with muscle tissues and amplification of its inflammation marker. The results revealed that the lichen compound (i.e., lecanoric acid) in the acetone extract of P. austrosinense possesses anti-inflammatory activity. Histopathology studies confirmed the decreased numbers of neutrophil cells in the 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced zebrafishes, as confirmed by changes in the fishes’ weight before and after the sample treatment, prompted by TNBS inflammation. The present results also demonstrated a dose-dependent decrease in the lipid peroxidation (LPO) levels in the muscle tissues of zebrafishes. Gene amplification studies suggested that the lichen compound might perform dose-dependent downregulation of the inflammatory gene marker of the tumor necrosis factor (TNF)-α gene; this further confirms that the extract should possess anti-inflammatory activity. As per the literature, this study is one of the most complete, comprehensive in vivo anti-inflammatory analyses in which inflammation was induced in zebrafish by using 2,4,6-trinitrobenzene sulfonic acid (TNBS). Particularly, this study successfully identified a bioactive compound isolated from the lichen P. austrosinense, and which exhibited decent anti-inflammatory activity.
Expert Systems for Farmed Fish Disease Diagnosis: An Overview and a Proposal
Authors: Hatzilygeroudis Ioannis , Dimitropoulos Konstantinos , Kovas Konstantinos , Theodorou John A.
Published: May 2023

The expert system approach, although quite old, is still quite effective in scientific areas where experts are required to make diagnoses and predictions. One of those areas is fish disease diagnosis. It is an application domain that currently employs complicated processes, which require high level skills for making accurate diagnoses. On the other hand, complete datasets for full diagnosis to be able to use machine learning techniques are not available. Therefore, in aquaculture, now more than ever, fish farmers do not have the required expertise or equipment to accurately diagnose a fish disease. For that reason, expert systems that can help in the diagnosis, prevention, and treatment of diseases have been developed. In this paper, we attempt to give an overview of the expert system approaches for fish disease diagnosis developed in the last two decades. Based on the analysis of their technical and non-technical characteristics, we propose an expert system architecture and a fish disease diagnosis process aiming at improving the deficiencies of the existing systems. The proposed system can handle all types of fish diseases based on image and non-image data as well as on molecular test results and can provide explanations. The diagnosis process goes through four consecutive levels, where each next level considers an additional category of parameters and provides diagnoses with a higher certainty.
Climatic Trend of Wind Energy Resource in the Antarctic
Authors: Wang Kai-Shan , Wu Di , Zhang Tao , Wu Kai , Zheng Chong-Wei , Yi Cheng-Tao , Yu Yue
Published: May 2023

Wind energy resource is an important support for the sustainable development of Antarctica. The evaluation of wind energy potential determines the feasibility and economy of wind power generation in Antarctica, among which mastering the variation rule of wind energy resource is the key to realizing the effective utilization of polar wind energy. Based on the 6-h ERA-5 reanalysis data of ECMWF from January 1981 to December 2020, this paper systematically analyzed the long-term variation trend of Antarctic wind energy resource by using the climate statistical analysis method and the least square fitting, with the comprehensive consideration of a series of key indicators such as Wind Power Density, Effective Wind Speed Occurrence, Energy Level Occurrence, and Stability. The results show that it indicates a positive trend for wind power density (0.5~2 W × m−2 × a−1), effective wind speed occurrence (2~3%/a), energy level occurrence (0.1~0.2%/a), and coefficient of variation (−0.005/a) in the South Pole—Kunlun station and the central region of Queen Maud land. The westerly belt exhibits a decreasing index (−0.5%/a) in terms of stability trend, indicating a positive potential. Kemp Land, the Ross Island—Balleny Islands waters show shortages in all indicators. The wind power density in the Antarctic region is stronger in spring and summer than in autumn and winter, with the weakest in autumn. Based on the above indicators, the variation trend in the East Antarctic coast, Wilhelm II Land—Wilkes Land, the South Pole—Kunlun station, and the westerlies is generally superior.
Highly Selective Targeting of Pancreatic Cancer in the Liver with a Near-Infrared Anti-MUC5AC Probe in a PDOX Mouse Model: A Proof-of-Concept Study
Authors: Turner Michael A. , Cox Kristin E. , Neel Nicholas , Amirfakhri Siamak , Nishino Hiroto , Clary Bryan M. , Hosseini Mojgan , Natarajan Gopalakrishnan , Mallya Kavita , Mohs Aaron M. , Hoffman Robert M. , Batra Surinder K. , Bouvet Michael
Published: May 2023

Accurately identifying metastatic disease is critical to directing the appropriate treatment in pancreatic cancer. Mucin 5AC is overexpressed in pancreatic cancer but absent in normal pancreas tissue. The present proof-of-concept study demonstrates the efficacy of an anti-mucin 5AC antibody conjugated to an IR800 dye (MUC5AC-IR800) to preferentially label a liver metastasis of pancreatic cancer (Panc Met) in a unique patient-derived orthotopic xenograft (PDOX) model. In orthotopic models, the mean tumor to background ratio was 1.787 (SD ± 0.336), and immunohistochemistry confirmed the expression of MUC5AC within tumor cells. MUC5AC-IR800 provides distinct visualization of pancreatic cancer liver metastasis in a PDOX mouse model, demonstrating its potential utility in staging laparoscopy and fluorescence-guided surgery.
Radiomics in Breast Imaging: Future Development
Authors: Panico Alessandra , Gatta Gianluca , Salvia Antonio , Grezia Graziella Di , Fico Noemi , Cuccurullo Vincenzo
Published: May 2023

Breast cancer is the most common and most commonly diagnosed non-skin cancer in women. There are several risk factors related to habits and heredity, and screening is essential to reduce the incidence of mortality. Thanks to screening and increased awareness among women, most breast cancers are diagnosed at an early stage, increasing the chances of cure and survival. Regular screening is essential. Mammography is currently the gold standard for breast cancer diagnosis. In mammography, we can encounter problems with the sensitivity of the instrument; in fact, in the case of a high density of glands, the ability to detect small masses is reduced. In fact, in some cases, the lesion may not be particularly evident, it may be hidden, and it is possible to incur false negatives as partial details that may escape the radiologist’s eye. The problem is, therefore, substantial, and it makes sense to look for techniques that can increase the quality of diagnosis. In recent years, innovative techniques based on artificial intelligence have been used in this regard, which are able to see where the human eye cannot reach. In this paper, we can see the application of radiomics in mammography.
Local Treatment of Triple-Negative Breast Cancer: Is Mastectomy Superior to Breast-Conserving Surgery?
Authors: Di Leone Alba , Franco Antonio , Zotta Francesca , Scardina Lorenzo , Sicignano Margherita , Di Guglielmo Enrico , Castagnetta Virginia , Magno Stefano , Terribile Daniela , Sanchez Alejandro Martin , Franceschini Gianluca , Masetti Riccardo
Published: May 2023

Triple-negative breast cancer (TNBC) is an aggressive type of breast cancer that lacks the expression of estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2). TNBC accounts for about 15% of breast cancers and has a poorer prognosis as compared with other subtypes of breast cancer. The more rapid onset of this cancer and its aggressiveness have often convinced breast surgeons that mastectomy could provide better oncological results. However, there is no relevant clinical trial that has assessed differences between breast-conserving surgery (BCS) and mastectomy (M) in these patients. This population-based study aimed to investigate the distinct outcomes between conservative treatment and M in a case series of 289 patients with TNBC treated over a 9-year period. This monocentric study retrospectively evaluated patients with TNBC who underwent upfront surgery at Fondazione Policlinico Agostino Gemelli IRCCS, in Rome, between 1 January 2013 and 31 December 2021. First, the patients were divided in two groups according to the surgical treatment received: BCS vs. M. Then, the patients were stratified into four risk subclasses based on combined T and N pathological staging (T1N0, T1N+, T2-4N0 and T2-4N+). The primary endpoint of the study was to evaluate locoregional disease-free survival (LR-DFS), distant disease-free survival (DDFS) and overall survival (OS) in the different subclasses. We analyzed 289 patients that underwent either breast-conserving surgery (247/289, 85.5%) or mastectomy (42/289, 14.5%). After a median follow-up of 43.2 months (49.7, 22.2–74.3), 28 patients (9.6%) developed a locoregional recurrence, 27 patients (9.0%) showed systemic recurrence and 19 patients (6.5%) died. No significant differences due to type of surgical treatment were observed in the different risk subclasses in terms of locoregional disease-free survival, distant disease-free survival and overall survival. With the limits of a retrospective, single-center study, our data seem to indicate similar efficacy in terms of locoregional control, distant metastasis and overall survival with the use of upfront breast-conserving surgery as compared with radical surgery in the treatment of TNBC. Therefore, TNBC should not be considered to be a contraindication for breast conservation.
Prostate Cancer Scoring Index for Risk of Progression of Radioresistant Disease
Authors: Tesar Eleonora Cini , Mikolasevic Ivana , Skocilic Iva , Redjovic Arnela , Vucinic Damir , Marusic Jasna , Djordjevic Gordana
Published: May 2023

Prostate cancer (Pca) is among the most common malignant diseases in men and the fourth leading cause of death worldwide. Surgery and radical radiotherapy (RT) remain the gold standard for the treatment of localized or locally advanced prostate cancer. The efficiency of radiotherapy treatment is limited by toxic side effects due to dose escalation. Cancer cells often develop radio-resistant mechanisms that are related to the DNA repair, inhibition of apoptosis or changes in cell cycle. Based on our earlier research on biomarkers that are involved in those cellular mechanisms (p53, bcl-2, NF-kb, Cripto-1 and Ki67 proliferation) and correlation with clinico-pathological parameters (age, PSA value, Gleason score, grade group, prognostic group), we created the numerical index for risk of tumor progression in patients with radioresistant tumors. For each of these parameters, the strength of association with disease progression was statistically assessed, and a specific number of points was assigned proportional to the strength of the correlation. Statistical analysis identified an optimal cut-off score of 22 or more as an indicator of significant risk for progression with a sensitivity of 91.7% and a specificity of 66.7%. The scoring system in the retrospective receiver operating characteristic analysis showed AUC of 0.82. The potential value of this scoring is the possibility of identifying patients with clinically significant radioresistant Pca.
The Role of Myocardial Perfusion Imaging in the Prediction of Major Adverse Cardiovascular Events at 1 Year Follow-Up: A Single Center’s Experience
Authors: Zotou Paraskevi , Bechlioulis Aris , Tsiouris Spyridon , Naka Katerina K. , Xourgia Xanthi , Pappas Konstantinos , Lakkas Lampros , Rammos Aidonis , Kalef-Ezra John , Michalis Lampros K. , Fotopoulos Andreas
Published: May 2023

Background: Myocardial perfusion imaging via single-photon emission computed tomography (SPECT MPI) is a well-established method of diagnosing coronary artery disease (CAD). The purpose of this study was to assess the role of SPECT MPI in predicting major cardiovascular events. Methods: The study population was composed of 614 consecutive patients (mean age: 67 years, 55% male) referred for SPECT MPI due to symptoms of stable CAD. The SPECT MPI was performed using a single-day protocol. We conducted a follow-up on all patients at 12 months via a telephone interview. Results: The majority of our patients (78%) presented findings suggestive of reversible ischemia, fixed defects or both. Extensive perfusion defects were found in 18% of the population, while LV dilation was found in 7%. During the 12-month follow-up, 16 deaths, 8 non-fatal MIs and 20 non-fatal strokes were recorded. There was no significant association of SPECT findings with the combined endpoint of all-cause death, non-fatal MI and non-fatal stroke. The presence of extensive perfusion defects was an independent predictor of mortality at 12 months (HR: 2.90, 95% CI: 1.05, 8.06, p = 0.041). Conclusions: In a high-risk patient population with suspected stable CAD, only large reversible perfusion defects in SPECT MPI were independently associated with mortality at 1 year. Further trials are needed to validate our findings and refine the role of SPECT MPI findings in the diagnosis and prognosis of cardiovascular patients.
The Role of Optical Transport Networks in 6G and Beyond: A Vision and Call to Action
Authors: Manias Dimitrios Michael , Javadtalab Abbas , Naoum-Sawaya Joe , Shami Abdallah
Published: May 2023

As next-generation networks begin to take shape, the necessity of Optical Transport Networks (OTNs) in helping achieve the performance requirements of future networks is evident. Future networks are characterized as being data-centric and are expected to have ubiquitous artificial intelligence integration and deployment. To this end, the efficient and timely transportation of fresh data from producer to consumer is critical. The work presented in this paper outlines the role of OTNs in future networking generations. Furthermore, key emerging OTN technologies are discussed. Additionally, the role intelligence will play in the Management and Orchestration (MANO) of next-generation OTNs is discussed. Moreover, a set of challenges and opportunities for innovation to guide the development of future OTNs is considered. Finally, a use case illustrating the impact of network dynamicity and demand uncertainty on OTN MANO decisions is presented.
Review of Valuation of Forest Ecosystem Services and Realization Approaches in China
Authors: Liu Shiliang , Dong Yuhong , Liu Hua , Wang Fangfang , Yu Lu
Published: May 2023

Forests are essential for the provision of water, financial resources, food, and carbon, and offer immense ecosystem service values. The accurate, quantitative, and objective evaluation of forest ecosystem service (FES) values can help uncover methods for realizing ecological product value, which in turn supports forest conservation and ecological benefit enhancement. In China, FES valuation methods are diverse and tailored to specific objectives, encompassing matter quantity assessment, value quantity assessment, energy value analysis, and landscape ecological modeling methods. The “Forest Ecosystem Service Function Assessment Specification” guideline plays a crucial role in fostering standardized valuation. Carbon-related ecosystem services have been increasingly studied in China; however, valuation challenges remain, including data accuracy, the double counting of ecosystem services, methodological limitations, and the incomplete assessment of non-use values. Regarding value realization, the development of payment for ecosystem services (PES), ecological product benefit trading (EPBT), ecological premiums, and ecological industries has seen gradual progresses in recent years. However, realization approaches still depend on government support, and the establishment of market-oriented strategies requires further reinforcement. Enhancing FES valuation necessitates the integration of interdisciplinary and multi-method approaches, as well as the creation of an accounting and assessment mechanism. Realization approaches must not only be continuously expanded but also consistently innovated over time. It is essential to consider the impact of market development on FES valuation; establish robust realization approaches; reinforce promotional and guarantee mechanisms; and increase the efficacy of policy management.
Assessing the Livelihood Vulnerability of Nomads to Changing Climate in the Third Pole Region of Nepal
Authors: Kayastha Rijan Bhakta , Lee Woo-Kyun , Shrestha Nischal , Wang Sonam Wangyel
Published: May 2023

This study was performed in Nepal’s Langtang and Shey Phoksundo National Parks (NPs) to find out how vulnerable the nomads’ ways of making a living are to climate change. We interviewed 68 household heads between March and May 2022 to obtain information on 13 components and 46 indicators. The original data were backed up by an analysis of the published and unpublished literature that was available. A composite index was used to combine the data, and different vulnerabilities were compared. As nomads in both NPs rely mostly on natural resources for energy, water, and food, the findings revealed that land, energy, water, sanitation, and natural resources are the most important factors influencing nomads’ livelihood vulnerability in both NPs. Although herders in Shey Phoksundo NP suffered less loss as a result of climate change-related natural disasters, human–wildlife conflict was a major issue in both parks. Both the livelihood vulnerability index (LVI) and LVI–IPCC suggested that both national parks were moderately vulnerable to climate change indicators. The results are likely to serve as empirical evidence for future strategies, such as implementing policy measures aimed at reducing the sensitivity of habitat conditions, increasing societal resilience, introducing sustainable livelihood alternatives, and improving individual stability.
Scale-Dependent Field Partition Based on Water Retention Functional Data
Authors: Castrignanò Annamaria , Heydari Ladan , Bayat Hossein
Published: May 2023

Functional data are being used increasingly in recent years and in many environmental sciences, such as hydrology applied to agriculture. This means that the output, instead of a scalar variable represented by a spatial map, is given by a function. Furthermore, in site-specific management, there is a need to delineate the field into management areas depending on the agricultural procedure and on the scale of application. In this paper, an approach based on multivariate geostatistics is illustrated that uses the parameters of Dexter’s water retention model and some soil properties to arrive at a multiscale delineation of an agricultural field in Iran. One hundred geo-referenced soil samples were taken and subjected to various measurements. The volumetric water contents at the different suctions were fitted to Dexter’s model. The estimated curve parameters plus the measurements of the soil variables were transformed into standardized Gaussian variables and the values transformed were subjected to geostatistical cokriging and factorial cokriging procedures. These results show that soil properties (organic carbon, bulk density, saturated hydraulic conductivity and tensile strength of soil aggregates) influence the parameters of Dexter’s model, although to different extents. The thematic maps of both soil properties and water retention curve parameters displayed a varying degree of spatial association that allowed the identification of homogeneous areas within the field. The first regionalized factors (F1) at the scales of 508 m and 3000 m made it possible to provide different delineations of the field into homogeneous areas as a function of scale, characterized by specific physical and hydraulic properties. F1 at a short and long distance could be interpreted as “porosity indicator” and “hydraulic indicator”, respectively. Such type of field delineation proves particularly useful in sustainable irrigation management. This paper emphasizes the importance of taking the spatial scale into account in precision agriculture.
Localization of the Catalytic Domain of Copepod Luciferases: Analysis of Truncated Mutants of the Metridia longa Luciferase
Authors: Markova Svetlana V. , Larionova Marina D. , Korotov Igor A. , Vysotski Eugene S.
Published: May 2023

Luciferases from copepods Metridia longa and Gaussia princeps are successfully used as bioluminescent reporters for in vivo and in vitro assays. Here, we report the minimal sequence of copepod luciferases required for bioluminescence activity that was revealed by gradual deletions of sequence encoding the smallest MLuc7 isoform of M. longa luciferase. The single catalytic domain is shown to reside within the G32-A149 MLuc7 sequence and to be formed by both non-identical repeats, including 10 conserved Cys residues. Because this part of MLuc7 displays high homology with those of other copepod luciferases, our suggestion is that the determined boundaries of the catalytic domain are the same for all known copepod luciferases. The involvement of the flexible C-terminus in the retention of the bioluminescent reaction product in the substrate-binding cavity was confirmed by structural modeling and kinetics study. We also demonstrate that the ML7-N10 mutant (15.4 kDa) with deletion of ten amino acid residues at the N-terminus can be successfully used as a miniature bioluminescent reporter in living cells. Application of a shortened reporter may surely reduce the metabolic load on the host cells and decrease steric and functional interference at its use as a part of hybrid proteins.
Further Validation of a Simple Mathematical Description of Wear and Contact Pressure Evolution in Sliding Contacts
Authors: Di Puccio Francesca , Mattei Lorenza
Published: May 2023

The present study proposes the further validation of a simple mathematical procedure recently proposed by the authors to describe contact and wear evolution in line and point contacts. The procedure assumed that the maximum contact pressure could be determined using Hertz equations and a parabolic pressure profile. The contact half-width was obtained using the equilibrium equation and the Archard wear law. Several cases were selected from the literature, reporting experimental data or Finite Element simulations, and the results were compared to those obtained with the proposed approach. This paper confirms the reliability and potentialities of the proposed analytical procedure, which is capable of providing accurate solutions in case of frictional contacts and at the borders of the contact area, where the main discrepancies were found in the previous study.
Thermal–Mechanical Coupling Analysis of Wheel–Rail Sliding Friction under Two-Point Contact Conditions
Authors: Han Feng , Wei Hao , Liu Yang
Published: May 2023

The generation of wheel–rail-sliding frictional heat is often accompanied by transverse displacement of a wheel. To study the thermal problem of wheel–rail sliding friction at two-point contact, this paper uses an LM tread wheel and a 60 kg·m−1 rail as examples. A thermal–mechanical-coupled finite-element model of equal proportion wheel–rail sliding is established. A direct-coupling method is used to analyze the thermal–mechanical coupling of the wheel–rail interface under sliding contact. This model considers the temperature-dependent material properties and boundary conditions, such as thermal convection and thermal dissipation, in the process of nonstationary frictional-heat conduction. Firstly, the effects of different sliding speeds, axle loads, and contact modes on the temperature and stress fields of the contact area are analyzed. Then, the lubrication and cooling effects of friction modifiers on the rail top and rail gauge angle are compared. The results show that, at a sliding speed of 2 m/s and an axle load of 30 t under a sliding condition of 200 mm, on the top and side of the rail, the temperatures at the contact patch centers are 813 °C and 547.7 °C, respectively. Under different operating conditions, the rail-side temperature is 55–75% of that of the temperature at the rail top, and the rail-side contact and friction stress values are 76–96% of those at the rail top. This indicates that frictional thermal damage on the rail side cannot be ignored. With a lower sliding speed, the thermal response of the two contact patches is closer. The impact of axle load on the frictional temperature and stress on the rail side is more critical than the sliding speed. The optimal lubrication choice is overall lubrication, which can decrease the rail top temperature by 47.2% and frictional stress by 56.2%, as well as decreasing the rail side temperature by 70.3% and frictional stress by 77.4%.
Comparative Analysis of Bone Ingrowth in 3D-Printed Titanium Lattice Structures with Different Patterns
Authors: Kovács Ágnes Éva , Csernátony Zoltán , Csámer Loránd , Méhes Gábor , Szabó Dániel , Veres Mihály , Braun Mihály , Harangi Balázs , Serbán Norbert , Zhang Lei , Falk György , Soósné Horváth Hajnalka , Manó Sándor
Published: May 2023

In this study, metal 3D printing technology was used to create lattice-shaped test specimens of orthopedic implants to determine the effect of different lattice shapes on bone ingrowth. Six different lattice shapes were used: gyroid, cube, cylinder, tetrahedron, double pyramid, and Voronoi. The lattice-structured implants were produced from Ti6Al4V alloy using direct metal laser sintering 3D printing technology with an EOS M290 printer. The implants were implanted into the femoral condyles of sheep, and the animals were euthanized 8 and 12 weeks after surgery. To determine the degree of bone ingrowth for different lattice-shaped implants, mechanical, histological, and image processing tests on ground samples and optical microscopic images were performed. In the mechanical test, the force required to compress the different lattice-shaped implants and the force required for a solid implant were compared, and significant differences were found in several instances. Statistically evaluating the results of our image processing algorithm, it was found that the digitally segmented areas clearly consisted of ingrown bone tissue; this finding is also supported by the results of classical histological processing. Our main goal was realized, so the bone ingrowth efficiencies of the six lattice shapes were ranked. It was found that the gyroid, double pyramid, and cube-shaped lattice implants had the highest degree of bone tissue growth per unit time. This ranking of the three lattice shapes remained the same at both 8 and 12 weeks after euthanasia. In accordance with the study, as a side project, a new image processing algorithm was developed that proved suitable for determining the degree of bone ingrowth in lattice implants from optical microscopic images. Along with the cube lattice shape, whose high bone ingrowth values have been previously reported in many studies, it was found that the gyroid and double pyramid lattice shapes produced similarly good results.
Nanostructured TiO2 Arrays for Energy Storage
Authors: Si Pingyun , Zheng Zhilong , Gu Yijie , Geng Chao , Guo Zhizhong , Qin Jiayi , Wen Wei
Published: May 2023

Because of their extensive specific surface area, excellent charge transfer rate, superior chemical stability, low cost, and Earth abundance, nanostructured titanium dioxide (TiO2) arrays have been thoroughly explored during the past few decades. The synthesis methods for TiO2 nanoarrays, which mainly include hydrothermal/solvothermal processes, vapor-based approaches, templated growth, and top-down fabrication techniques, are summarized, and the mechanisms are also discussed. In order to improve their electrochemical performance, several attempts have been conducted to produce TiO2 nanoarrays with morphologies and sizes that show tremendous promise for energy storage. This paper provides an overview of current developments in the research of TiO2 nanostructured arrays. Initially, the morphological engineering of TiO2 materials is discussed, with an emphasis on the various synthetic techniques and associated chemical and physical characteristics. We then give a brief overview of the most recent uses of TiO2 nanoarrays in the manufacture of batteries and supercapacitors. This paper also highlights the emerging tendencies and difficulties of TiO2 nanoarrays in different applications.
Machining of Triangular Holes in D2 Steel by the Use of Non-Conventional Electrodes in Die-Sinking Electric Discharge Machining
Authors: Rafaqat Madiha , Mufti Nadeem Ahmad , Saleem Muhammad Qaiser , Ahmed Naveed , Rehman Ateekh Ur , Ali Muhammad Asad
Published: May 2023

Electric discharge machining is relatively a slow process in terms of machining time and material removal rate. The presence of overcut and the hole taper angle caused by the excessive tool wear are other challenges in the electric discharge machining die-sinking process. The areas of focus to solve these challenges in the performance of electric discharge machines include increasing the rate of material removal, decreasing the rate of tool wear, and reducing the rate of hole taper angle and overcut. Triangular cross-sectional through-holes have been produced in D2 steel through die-sinking electric discharge machining (EDM). Conventionally, the electrode with uniform triangular cross-section throughout the electrode length is used to machine triangular holes. In this study, new designs of electrodes (non-conventional designs) are employed by introducing circular relief angles. For material removal rate (MRR), tool wear rate (TWR), overcut, taper angle, and surface roughness of the machined holes, the machining performance of conventional and unconventional electrode designs is compared. A significant improvement in MRR (32.6% increase) has been achieved by using non-conventional electrode designs. Similarly, the hole quality resulted by non-conventional electrodes is way better than hole quality corresponding to conventional electrode designs, especially in terms of overcut and hole taper angle. A reduction of 20.6% in overcut and a reduction of 72.5% in taper angle can be achieved through newly designed electrodes. Finally, one electrode design has been selected (electrode with 20 degree relief angle) as the most appropriate electrode resulting in better EDM performance in terms of MRR, TWR, overcut, taper angle, and surface roughness of triangular holes.