bluuebunny/crossref_metadata_2025 huggingface dataset:
Direct Action of Non-Digestible Oligosaccharides against a Leaky Gut
Authors: Mavrogeni Maria Eleni , Asadpoor Mostafa , Henricks Paul A. J. , Keshavarzian Ali , Folkerts Gert , Braber Saskia
Published: November 2022

The epithelial monolayer is the primary determinant of mucosal barrier function, and tight junction (TJ) complexes seal the paracellular space between the adjacent epithelial cells and represent the main “gate-keepers” of the paracellular route. Impaired TJ functionality results in increased permeation of the “pro-inflammatory” luminal contents to the circulation that induces local and systemic inflammatory and immune responses, ultimately triggering and/or perpetuating (chronic) systemic inflammatory disorders. Increased gut leakiness is associated with intestinal and systemic disease states such as inflammatory bowel disease and neurodegenerative diseases such as Parkinson’s disease. Modulation of TJ dynamics is an appealing strategy aiming at inflammatory conditions associated with compromised intestinal epithelial function. Recently there has been a growing interest in nutraceuticals, particularly in non-digestible oligosaccharides (NDOs). NDOs confer innumerable health benefits via microbiome-shaping and gut microbiota-related immune responses, including enhancement of epithelial barrier integrity. Emerging evidence supports that NDOs also exert health-beneficial effects on microbiota independently via direct interactions with intestinal epithelial and immune cells. Among these valuable features, NDOs promote barrier function by directly regulating TJs via AMPK-, PKC-, MAPK-, and TLR-associated pathways. This review provides a comprehensive overview of the epithelial barrier-protective effects of different NDOs with a special focus on their microbiota-independent modulation of TJs.
The Contribution of Diet Therapy and Probiotics in the Treatment of Sarcopenia Induced by Prolonged Immobilization Caused by the COVID-19 Pandemic
Authors: Nistor-Cseppento Carmen Delia , Moga Titus David , Bungau Alexa Florina , Tit Delia Mirela , Negrut Nicoleta , Pasca Bianca , Bochis Calin Florin , Ghitea Timea Claudia , Jurcau Anamaria , Purza Anamaria Lavinia , Uivarosan Diana
Published: November 2022

The prolonged immobilization associated with COVID-19 infection and the restrictions imposed by the pandemic have determined major changes in physical activity and eating habits, with a negative impact on physical performance. This study monitored non-pharmacological interventions (diet therapy and probiotics) in managing sarcopenia for patients with recent SARS-CoV-2 history (14 days). A prospective study was performed on 200 patients (between December 2020–December 2021), with SPPB score < 9, randomly divided into: Group K—DP (93 patients) with dietary therapy (protein 1.2–1.5 g/kg) and probiotics for two months; and Group K—non-DP (107 patients) without diet therapy and probiotics. All patients were included in a specific physical training program (40 min), three sessions per week. Skeletal muscle index (SMI), serum albumin, and hemoglobin were determined. The SMI was initially low for both groups without significant statistical differences (6.5 ± 0.52 kg/m2 for Group K—non-DP vs. 6.7 ± 0.57 Kg/m2 for Group K—DP, p = 0.135). After two months, significant difference between initial and final SMI values was determined for Group K—DP (6.92 ± 0.50 kg/m2 vs. 6.77 ± 0.56 kg/m2, p = 0.048). In Group K—DP, at end of study, were more patients with normal SMI (n = 32 → N = 70) values (p < 0.001) and fewer sarcopenia patients (p < 0.001). The initial serum albumin means values in the two groups (Group K—non-DP, 4.17 ± 1.04 g/dL, and Group K—DP, 3.95 ± 0.98 g/dL) were not statistically significantly different (p = 0.122). The hemoglobin level improved significantly following a hyper protein diet enriched with pro-biotics (p = 0.003). Diet therapy, consisting of increased protein intake and specific probiotics and specific physical therapy, demonstrated superiority in improving the functional status of patients with recent COVID-19 infection.
Are Foods from the COVID-19 Pandemic Lockdown Low in Nutrients? An Analysis of Chinese Psychological Distress Effects
Authors: Jiao Wen , Xiang Yu-Tao , Chang Angela
Published: November 2022

Background: The city-wide COVID-19 lockdown has resulted in psychological anguish, which may have an impact on dietary consumption. This study’s dual goals are to show how Chinese food consumption was altered before and after the lockdown, and to examine the nutrient density for the psychologically affected group. Methods: A cross-sectional study involving 652 people from Mainland China, Taiwan, and Macao was conducted with the aid of a web-based questionnaire. Sociodemographic characteristics, related environmental factors, nutrient consumption, food recommendations, and psychological distress were all measured. 516 trustworthy data revealed that two nutrient-poor foods were consumed less frequently during the lockdown than they were before to the COVID-19 outbreak (i.e., salty snacks and alcoholic beverages). People who endured high levels of psychological distress in particular tended to consume more. Particularly, those who experienced high levels of psychological distress had a tendency to consume far more alcohol than people who only experienced low levels of stress. Comparing the time before the COVID-19 to the present, there has statistically been an increase in the frequency of family members recommending diets. According to research, by food advice, individuals who experience psychological distress should consume more nutrient-dense foods (78.7%) than nutrient-poor ones (61.9%). Thus, food advice plays a role in mediating the relationship between psychological distress and dietary decisions for nutrient-rich (b = 0.186, p < 0.001) or nutrient-poor (b = 0.187, p < 0.001) food groups. This study provides insights for lowering psychological distress through dietary consumption, where the exact mechanisms underlying these connections have not been thoroughly elucidated. It encourages nutrition research by recommending practical nutrition education from family and environmental activities. Chronic psychological anguish may have a crucial relationship to secure access to food and a balanced diet. Along with nutrition instruction, it is critical to develop skills in interventions such as food procurement and culinary knowledge.
Strategies to Improve Adolescent Food Security from the Perspectives of Policy Advocates, Parents, and Adolescents
Authors: Harper Kaitlyn , Skinner Rebecca , Martinez-Baack Michelle , Caulfield Laura E. , Gross Susan M. , Mmari Kristin
Published: November 2022

This study explored strategies to improve adolescent food security using semi-structured in-depth interviews with 9 policy advocates, 12 parents and 15 adolescents aged between 17 and 20 years, living in households who were eligible for the Supplemental Nutrition Assistance Program in 2020. This study was part of a larger evaluation of adolescent food insecurity conducted in Baltimore, Maryland, USA during the COVID-19 pandemic. Three key strategies arose during analysis—improving federal nutrition assistance programs for households, federal nutrition assistance programs for individual adolescents, and leveraging school programs and resources. Respondents described concordant views regarding the role of the Supplemental Nutrition Assistance Program in supporting households but held discordant views about the role of other federal programs, such as the school nutrition programs and Pandemic Electronic Benefit Transfer program. The results of this study provide important insights about policy and programmatic supports that may assist adolescents to acquire food for themselves and their families. Future research should test how federal programs and policies specifically impact food security and nutrition for adolescents.
The Income Elasticities of Food, Calories, and Nutrients in China: A Meta-Analysis
Authors: Zhao Jinlu , Huang Jiaqi , Nie Fengying
Published: November 2022

Estimating food- and nutrient-income elasticities is important for making food and nutrition policies to combat malnutrition. There are many studies that have estimated the relationship between income growth and food/nutrient demand in China, but the results are highly heterogeneous. We conducted a meta-analysis in China to systematically review the elasticity of food, calories, and other nutrients to income. We considered a meta-sample using a collection of 64 primary studies covering 1537 food-income elasticities, 153 nutrient-income elasticities, and 147 calorie-income elasticity estimates. There are significant differences in the size of the income elasticities across food and nutrient groups. We found that food- and calorie-income elasticity appear to decline as per capita income increases, except for vitamin and aquatic products. We also found a publication bias for food and calories, and in particular, the study attributes may be important, as they can influence estimates. Given the limited study on nutrient-income elasticity, understanding the impact of income changes on nutrient intake is an important direction worthy of further research.
The Relationship between Serum Adipokines, miR-222-3p, miR-103a-3p and Glucose Regulation in Pregnancy and Two to Three Years Post-Delivery in Women with Gestational Diabetes Mellitus Adhering to Mediterranean Diet Recommendations
Authors: Valerio Johanna , Barabash Ana , Garcia de la Torre Nuria , De Miguel Paz , Melero Verónica , del Valle Laura , Moraga Inmaculada , Familiar Cristina , Durán Alejandra , Torrejón Maria Jose , Diaz Angel , Jiménez Inés , Matia Pilar , Rubio Miguel Angel , Calle-Pascual Alfonso Luis
Published: November 2022

The San Carlos Gestational Diabetes Mellitus (GDM) prevention study, a nutritional intervention RCT based on a Mediterranean Diet (MedDiet), has been shown to reduce the incidence of GDM. The objective of this study is to investigate the relationship of leptin, adiponectin, interleukin-6 (IL-6), tumour necrosis factor-alpha (TNF-α), insulin and HOMA-IRand circulating miRNAs (miR-29a-3p, miR-103a-3p, miR-132-3p, miR-222-3p) with the appearance of GDM and with MedDiet-based nutritional intervention, at 24–28 gestational weeks (GW), and in glucose regulation 2–3 years post-delivery (PD). A total of 313 pregnant women, 77 with GDM vs. 236 with normal glucose tolerance (NGT), 141 from the control group (CG, MedDiet restricting the consumption of dietary fat including EVOO and nuts during pregnancy) vs. 172 from the intervention group (IG, MedDiet supplemented with extra virgin olive oil (EVOO) and pistachios during pregnancy) were compared at Visit 1 (8–12 GW), Visit 2 (24–28 GW) and Visit 3 (2–3 years PD). Expression of miRNAs was determined by the Exiqon miRCURY LNA RT-PCR system. Leptin, adiponectin, IL-6 and TNF-α, were measured by Milliplex® immunoassays on Luminex 200 and insulin by RIA. Women with GDM vs. NTG had significantly higher leptin median (Q1–Q3) levels (14.6 (9.2–19.4) vs. 9.6 (6.0–15.1) ng/mL; p < 0.05) and insulin levels (11.4 (8.6–16.5) vs. 9.4 (7.0–12.8) µUI/mL; p < 0.001) and lower adiponectin (12.9 (9.8–17.2) vs. 17.0 (13.3–22.4) µg/mL; p < 0.001) at Visit 2. These findings persisted in Visit 3, with overexpression of miR-222-3p (1.45 (0.76–2.21) vs. 0.99 (0.21–1.70); p < 0.05)) and higher levels of Il-6 and TNF-α. When the IG is compared with the CG lower levels of insulin, HOMA-IR-IR, IL-6 levels at Visit 2 and 3 and leptin levels only at Visit 2 were observed. An overexpression of miR-222-3p and miR-103a-3p were also observed in IG at Visit 2 and 3. The miR-222-3p and miR103a-3p expression correlated with insulin levels, HOMA-IR, IL-6 and TNF-α at Visit 2 (all p < 0.05). These data support the association of leptin, adiponectin and insulin/HOMA-IR with GDM, as well as the association of insulin/HOMA-IR and IL-6 and miR-222-3p and miR-103a-3p expression with a MedDiet-based nutritional intervention.
Maternal Consumption of Dairy Products during Pregnancy Is Associated with Decreased Risk of Emotional Problems in 5-Year-Olds: The Kyushu Okinawa Maternal and Child Health Study
Authors: Nguyen Mai Quynh , Miyake Yoshihiro , Tanaka Keiko , Hasuo Shizuka , Takahashi Keiji , Nakamura Yoshitaka , Okubo Hitomi , Sasaki Satoshi , Arakawa Masashi
Published: November 2022

Milk is a good source of fats, minerals, and vitamins. The present prebirth cohort study examined the association between maternal dairy product intake during pregnancy and the risk of childhood behavioral problems in 5-year-old Japanese children. Study subjects were 1199 mother–child pairs. Dietary intake was assessed using a diet history questionnaire. Emotional problems, conduct problems, hyperactivity problems, peer problems, and low prosocial behavior were assessed using the parent-reported version of the Strengths and Difficulties Questionnaire. Adjustments were made for a priori selected non-dietary confounders and potentially related dietary factors. A significant inverse exposure–response association was observed between maternal total dairy intake during pregnancy and the risk of childhood emotional problems (adjusted odds ratio [OR] between extreme quartiles, 0.62; 95% confidence interval [CI]: 0.36–1.03, p for trend, 0.04). The greater maternal consumption of cow’s milk, but not yogurt or cheese, during pregnancy was independently related to a reduced risk of emotional problems in children (adjusted OR between extreme quartiles, 0.41; 95% CI: 0.23–0.70, p for trend, 0.003). Higher maternal consumption levels of total dairy products, especially cow’s milk, during pregnancy may be associated with a decreased risk of emotional problems in 5-year-old children.
Tracking of Dietary Patterns in the Secondary Prevention of Cardiovascular Disease after a Nutritional Intervention Program—A Randomized Clinical Trial
Authors: Coura Amanda Gonçalves Lopes , Arruda Neta Adélia da Costa Pereira de , Lima Rafaela Lira Formiga Cavalcanti de , Bersch-Ferreira Ângela Cristine , Weber Bernardete , Vianna Rodrigo Pinheiro de Toledo
Published: November 2022

Individuals with a history of previous cardiovascular events have an increased risk of mortality and morbidity, so adherence to a healthy dietary pattern is essential. We aimed to evaluate and compare dietary patterns between the control and the experimental group from the BALANCE Program. A total of 2360 individuals aged 45 years or older with previous cardiovascular disease were included. The individuals were randomized into two groups: intervention (dietary prescription with nutritional recommendations, nutritional education program based on playful strategies, suggestions of typical and accessible Brazilian foods and intensive monitoring) and control (conventional nutritional counseling). The dietary patterns were identified using factor analysis with the principal component extraction method, and the t-Student tests and ANOVA test were performed to evaluate the associated factors. Four dietary patterns were identified for both groups: “Traditional”, “Snack”, “Western”, “Cardioprotective”. There was an increase in the variances of the “Cardioprotective” pattern in both groups. Regarding the “Western” pattern, there was a significant reduction in the variances of the experimental group (10.63% vs. 8.14%). Both groups had improvements in eating habits, especially in the first year of follow-up. The greater increase in adherence to the traditional and cardioprotective pattern in the experimental group justifies the initiative of the BALANCE program.
Current Strategies for Selenium and Iodine Biofortification in Crop Plants
Authors: Duborská Eva , Šebesta Martin , Matulová Michaela , Zvěřina Ondřej , Urík Martin
Published: November 2022

Selenium and iodine are essential trace elements for both humans and animals. Among other things, they have an essential role in thyroid function and the production of important hormones by the thyroid gland. Unfortunately, in many areas, soils are deficient in selenium and iodine, and their amount is insufficient to produce crops with adequate contents to cover the recommended daily intake; thus, deficiencies have an endemic character. With the introduction of iodized table salt in the food industry, the thyroid status of the population has improved, but several areas remain iodine deficient. Furthermore, due to the strong relationship between iodine and selenium in metabolic processes, selenium deficiency often compromises the desired positive impact of salt iodization efforts. Therefore, a considerable number of studies have looked for alternative methods for the simultaneous supplementation of selenium and iodine in foodstuff. In most cases, the subject of these studies is crops; recently, meat has also been a subject of interest. This paper reviews the most recent strategies in agriculture to fortify selenium and iodine in crop plants, their effect on the quality of the plant species used, and the potential impact of food processing on their stability in fortified crops.
Potential Therapeutic Benefits of Metformin Alone and in Combination with Sitagliptin in the Management of Type 2 Diabetes Patients with COVID-19
Authors: Al-Kuraishy Hayder M. , Al-Gareeb Ali I. , Albogami Sarah M. , Jean-Marc Sabatier , Nadwa Eman Hassan , Hafiz Amin A. , A. Negm Walaa , Kamal Marwa , Al-Jouboury Mohammed , Elekhnawy Engy , Batiha Gaber El-Saber , Waard Michel De
Published: November 2022

Type 2 diabetes mellitus (T2DM) is a potential risk factor for the development of COVID-19 and is associated with higher severity and mortality rates. T2DM patients are commonly treated with metformin monotherapy or metformin plus sitagliptin. In the present case-control, single-center cohort study, a total number of 112 T2DM patients suffering from COVID-19 and aged 44–62 years old were compared with 78 T2DM patients without COVID-19 and aged 42–56 years old. Both the patient group and the control group were allocated into four groups. Group A: T2DM patients with COVID-19 on metformin treatments plus standard therapy (n = 60); group B: T2DM patients with COVID-19 on metformin plus sitagliptin plus standard therapy (n = 52); group C: T2DM patients without COVID-19 on metformin treatments (n = 40); and group D: T2DM patients without COVID-19 on metformin plus sitagliptin (n = 38). The investigation duration was 2–3 weeks. Anthropometric measurements, serological and biochemical investigations, pulmonary radiological findings, and clinical outcomes were evaluated. Only 101 T2DM patients with COVID-19 continued the study, 71 (70.29%) with mild-moderate COVID-19 and 30 (29.7%) with severe COVID-19 were compared with 78 T2DM patients as a control. Inflammatory biomarkers (C reactive protein, ferritin, and procalcitonin), a lung injury biomarker (lactate dehydrogenase), and a coagulopathy biomarker (D-dimer) were elevated in severe COVID-19 patients compared with mild-moderate COVID-19 (p < 0.05) and T2DM patients (p < 0.05). However, metformin plus sitagliptin was more effective than metformin monotherapy in T2DM patients with COVID-19, as evidenced by the mitigation of oxidative stress, CT scan score, and clinical outcomes. The present study confirmed the protective effects of this combination against the development of COVID-19 severity, as most T2DM COVID-19 patients develop mild-moderate forms. Herein, the combination of metformin and sitagliptin may lead to more beneficial effects than metformin monotherapy.
A Review of the Safety of Interleukin-17A Inhibitor Secukinumab
Authors: Eshwar Vishnu , Kamath Ashwin , Shastry Rajeshwari , Shenoy Ashok K. , Kamath Priyanka
Published: November 2022

Secukinumab is an anti-interleukin (IL)-17A IgG1-κ monoclonal antibody approved for psoriasis, psoriatic arthritis, and ankylosing spondylitis. Its efficacy is well documented, but the complete safety profile of secukinumab, especially on long-term use, needs to be studied. IL-17 inhibitors increase the risk of infections, especially respiratory tract infections and candidiasis, and inflammatory bowel disease; the causal relationships are well described. However, evidence regarding the other adverse events is scarce, and causal associations between the adverse events and the biologic remain unresolved. This review aims to present a narrative perspective on the safety of secukinumab and identify some key areas where the safety of secukinumab may potentially be useful in understanding the scope of secukinumab therapy and making informed clinical decisions.
Reactivity of Covalent Fragments and Their Role in Fragment Based Drug Discovery
Authors: McAulay Kirsten , Bilsland Alan , Bon Marta
Published: November 2022

Fragment based drug discovery has long been used for the identification of new ligands and interest in targeted covalent inhibitors has continued to grow in recent years, with high profile drugs such as osimertinib and sotorasib gaining FDA approval. It is therefore unsurprising that covalent fragment-based approaches have become popular and have recently led to the identification of novel targets and binding sites, as well as ligands for targets previously thought to be ‘undruggable’. Understanding the properties of such covalent fragments is important, and characterizing and/or predicting reactivity can be highly useful. This review aims to discuss the requirements for an electrophilic fragment library and the importance of differing warhead reactivity. Successful case studies from the world of drug discovery are then be examined.
Cytoskeletal and Cytoskeleton-Associated Proteins: Key Regulators of Cancer Stem Cell Properties
Authors: Li Yuqiang , Wang Dan , Ge Heming , Güngör Cenap , Gong Xuejun , Chen Yongheng
Published: November 2022

Cancer stem cells (CSCs) are a subpopulation of cancer cells possessing stemness characteristics that are closely associated with tumor proliferation, recurrence and resistance to therapy. Recent studies have shown that different cytoskeletal components and remodeling processes have a profound impact on the behavior of CSCs. In this review, we outline the different cytoskeletal components regulating the properties of CSCs and discuss current and ongoing therapeutic strategies targeting the cytoskeleton. Given the many challenges currently faced in targeted cancer therapy, a deeper comprehension of the molecular events involved in the interaction of the cytoskeleton and CSCs will help us identify more effective therapeutic strategies to eliminate CSCs and ultimately improve patient survival.
Features of Peripheral Blood Th-Cell Subset Composition and Serum Cytokine Level in Patients with Activity-Driven Ankylosing Spondylitis
Authors: Shesternya Pavel A. , Savchenko Andrei A. , Gritsenko Olga D. , Vasileva Alexandra O. , Kudryavtsev Igor V. , Masterova Alena A. , Isakov Dmitry V. , Borisov Alexandr G.
Published: November 2022

Th cells may exhibit pathological activity depending on the regulatory and functional signals sensed under a wide range of immunopathological conditions, including ankylosing spondylitis (AS). The relationship between Th cells and cytokines is important for diagnoses and for determining treatment. Accordingly, the aim of this study was to investigate the relationship between Th-cell subset composition and serum cytokine profile for patients with activity-driven AS. In our study, patients were divided into two groups according to disease activity: low-activity AS (ASDAS-CRP < 2.1) and high-activity AS (ASDAS-CRP > 2.1). The peripheral blood Th cell subset composition was studied by flow cytometry. Using multiplex analysis, serum cytokine levels were quantified and investigated. It was found that only patients with high-activity AS had reduced central memory (CM) Th1 cells (p = 0.035) but elevated numbers of CM (p = 0.014) and effector memory (EM) Th2 cells (p < 0.001). However, no activity-driven change in the Th17 cell subset composition was observed in AS patients. Moreover, low-AS activity patients had increased numbers of Tfh17 EM cells (p < 0.001), whereas high-AS activity was associated with elevated Tfh2 EM level (p = 0.031). The serum cytokine profiles in AS patients demonstrated that cues stimulating cellular immunity were increased, but patients with high-AS activity reveled increased IL-5 level (p = 0.017). Analyzing the data obtained from AS patients allowed us to conclude that Th cell subset differentiation was mainly affected during the CM stage and characterized the IL-23/IL-17 regulatory axis, whereas increased humoral immunity was observed in the high-AS activity group.
Controlled Release of Bone Morphogenetic Protein-2 Augments the Coupling of Angiogenesis and Osteogenesis for Accelerating Mandibular Defect Repair
Authors: Yao Hao , Guo Jiaxin , Zhu Wangyong , Su Yuxiong , Tong Wenxue , Zheng Lizhen , Chang Liang , Wang Xinluan , Lai Yuxiao , Qin Ling , Xu Jiankun
Published: November 2022

Reconstruction of a mandibular defect is challenging, with high expectations for both functional and esthetic results. Bone morphogenetic protein-2 (BMP-2) is an essential growth factor in osteogenesis, but the efficacy of the BMP-2-based strategy on the bone regeneration of mandibular defects has not been well-investigated. In addition, the underlying mechanisms of BMP-2 that drives the bone formation in mandibular defects remain to be clarified. Here, we utilized BMP-2-loaded hydrogel to augment bone formation in a critical-size mandibular defect model in rats. We found that implantation of BMP-2-loaded hydrogel significantly promoted intramembranous ossification within the defect. The region with new bone triggered by BMP-2 harbored abundant CD31+ endomucin+ type H vessels and associated osterix (Osx)+ osteoprogenitor cells. Intriguingly, the new bone comprised large numbers of skeletal stem cells (SSCs) (CD51+ CD200+) and their multi-potent descendants (CD51+ CD105+), which were mainly distributed adjacent to the invaded blood vessels, after implantation of the BMP-2-loaded hydrogel. Meanwhile, BMP-2 further elevated the fraction of CD51+ CD105+ SSC descendants. Overall, the evidence indicates that BMP-2 may recapitulate a close interaction between functional vessels and SSCs. We conclude that BMP-2 augmented coupling of angiogenesis and osteogenesis in a novel and indispensable way to improve bone regeneration in mandibular defects, and warrants clinical investigation and application.
Itraconazole Amorphous Solid Dispersion Tablets: Formulation and Compaction Process Optimization Using Quality by Design Principles and Tools
Authors: Triboandas Hetvi , Pitt Kendal , Bezerra Mariana , Ach-Hubert Delphine , Schlindwein Walkiria
Published: November 2022

BCS Class II drugs, such as itraconazole (ITZ), exhibit poor solubility (1–4 ng/mL) and so require solubility enhancement. Therefore, ITZ and Kollidon® VA64 (KOL) amorphous solid dispersions (ASDs) were produced using hot-melt extrusion (HME) to improve ITZ’s poor solubility. A novel strategy for tablet formulations using five inorganic salts was investigated (KCl, NaCl, KBr, KHCO3 and KH2PO4). These kosmotopric salts are thought to compete for water hydration near the polymer chain, hence, preventing polymer gelation and, therefore, facilitating disintegration and dissolution. Out of all the formulations, the KCl containing one demonstrated acceptable tensile strength (above 1.7 MPa), whilst providing a quick disintegration time (less than 15 min) and so was selected for further formulation development through a design of the experiment approach. Seven ITZ-KOL-ASD formulations with KCl were compacted using round and oblong punches. Round tablets were found to disintegrate under 20 min, whereas oblong tablets disintegrated within 10 min. The round tablets achieved over 80% ITZ release within 15 min, with six out of seven formulations achieving 100% ITZ release by 30 min. It was found that tablets comprising high levels of Avicel® pH 102 (30%) and low levels of KCl (5%) tend to fail the disintegration target due to the strong bonding capacity of Avicel® pH 102. The disintegration time and tensile strength responses were modeled to obtain design spaces (DSs) relevant to both round and oblong tablets. Within the DS, several formulations can be chosen, which meet the Quality Target Product Profile (QTPP) requirements for immediate-release round and oblong tablets and allow for flexibility to compact in different tablet shape to accommodate patients’ needs. It was concluded that the use of inorganic salts, such as KCl, is the key to producing tablets of ITZ ASDs with fast disintegration and enhanced dissolution. Overall, ITZ-KOL-ASD tablet formulations, which meet the QTPP, were achieved in this study with the aid of Quality by Design (QbD) principles for formulation and compaction process development and optimization.
Quality by Design Based Formulation of Xanthohumol Loaded Solid Lipid Nanoparticles with Improved Bioavailability and Anticancer Effect against PC-3 Cells
Authors: Harish Vancha , Tewari Devesh , Mohd Sharfuddin , Govindaiah Pilli , Babu Malakapogu Ravindra , Kumar Rajesh , Gulati Monica , Gowthamarajan Kuppusamy , Madhunapantula SubbaRao V. , Chellappan Dinesh Kumar , Gupta Gaurav , Dua Kamal , Dallavalasa Siva , Singh Sachin Kumar
Published: November 2022

Many natural products with greater therapeutic efficacy are limited to target several chronic diseases such as cancer, diabetes, and neurodegenerative diseases. Among the natural products from hops, i.e., Xanthohumol (XH), is a prenylated chalcone. The present research work focuses on the enhancement of the poor oral bioavailability and weak pharmacokinetic profile of XH. We exemplified the development of a Xanthohumol-loaded solid lipid nanoparticles (XH-SLNs) cargo system to overcome the limitations associated with its bioavailability. The XH-SLNs were prepared by a high-shear homogenization/ultrasonication method and graphical, numerical optimization was performed by using Box–Behnken Design. Optimized XH-SLNs showed PS (108.60 nm), PDI (0.22), ZP (−12.70 mV), %EE (80.20%) and an amorphous nature that was confirmed by DSC and PXRD. FE-SEM and HRTEM revealed the spherical morphology of XH-SLNs. The results of release studies were found to be 9.40% in 12 h for naive XH, whereas only 28.42% of XH was released from XH-SLNs. The slow release of drugs may be due to immobilization of XH in the lipid matrix. In vivo pharmacokinetic study was performed for the developed XH-SLNs to verify the enhancement in the bioavailability of XH than naive XH. The enhancement in the bioavailability of the XH was confirmed from an increase in Cmax (1.07-folds), AUC0-t (4.70-folds), t1/2 (6.47-folds) and MRT (6.13-folds) after loading into SLNs. The relative bioavailability of XH loaded in SLNs and naive XH was found to be 4791% and 20.80%, respectively. The cytotoxicity study of naive XH, XH-SLNs were performed using PC-3 cell lines by taking camptothecin as positive control. The results of cytotoxicity study revealed that XH-SLNs showed good cell inhibition in a sustained pattern. This work successfully demonstrated formulation of XH-SLNs with sustained release profile and improved oral bioavailability of XH with good anticancer properties against PC-3 cells.
A Review on the Role of Earthworms in Plastics Degradation: Issues and Challenges
Authors: Khaldoon Shahad , Lalung Japareng , Maheer Umrana , Kamaruddin Mohamad Anuar , Yhaya Mohd Firdaus , Alsolami Eman S. , Alorfi Hajer S. , Hussein Mahmoud A. , Rafatullah Mohd
Published: November 2022

Recently, the contribution of earthworms to plastic degradation and their capability to swallow smaller plastic fragments, known as microplastics, has been emphasized. The worm physically changes the size of microplastics and enhances microbial activities to increase the possibility of degradation. However, no research has shown that earthworms can chemically degrade microplastics to an element form, CO2 or H2O. In this review, previous research has been thoroughly explored to analyse the role that earthworms could play in plastic degradation in the soil. Earthworms can significantly affect the physical characteristics of plastics. However, earthworms’ abilities to chemically degrade or change the chemical structure of plastics and microplastics have not been observed. Additionally, earthworms exhibit selective feeding behaviour, avoiding areas containing a high plastics concentration and rejecting plastics. Consequently, earthworms’ abilities to adapt to the microplastics in soil in the environment can cause a problem. Based on this review, the challenges faced in earthworm application for plastic degradation are mostly expected to be associated with the toxicity and complexity of the plastic material and environmental factors, such as the moisture content of the soil and its temperature, microbial population, and feeding method.
Low Impact Velocity Modeling of 3D Printed Spatially Graded Elastomeric Lattices
Authors: Diosdado-De la Peña Jose Angel , Dwyer Charles M. , Krzeminski David , MacDonald Eric , Saldaña-Robles Alberto , Cortes Pedro , Choo Kyosung
Published: November 2022

Additive manufacturing technologies have facilitated the construction of intricate geometries, which otherwise would be an extenuating task to accomplish by using traditional processes. Particularly, this work addresses the manufacturing, testing, and modeling of thermoplastic polyurethane (TPU) lattices. Here, a discussion of different unit cells found in the literature is presented, along with the based materials used by other authors and the tests performed in diverse studies, from which a necessity to improve the dynamic modeling of polymeric lattices was identified. This research focused on the experimental and numerical analysis of elastomeric lattices under quasi-static and dynamic compressive loads, using a Kelvin unit cell to design and build non-graded and spatially side-graded lattices. The base material behavior was fitted to an Ogden 3rd-order hyperelastic material model and used as input for the numerical work through finite element analysis (FEA). The quasi-static and impact loading FEA results from the lattices showed a good agreement with the experimental data, and by using the validated simulation methodology, additional special cases were simulated and compared. Finally, the information extracted from FEA allowed for a comparison of the performance of the lattice configurations considered herein.
Grating Assembly Dissected in Periodic Bands of Poly (Butylene Adipate) Modulated with Poly (Ethylene Oxide)
Authors: Chang Chia-I. , Woo Eamor M. , Nagarajan Selvaraj
Published: November 2022

Polarized optical microscopy (POM), scanning electron microscopy (SEM), and synchrotron microbeam wide-angle X-ray diffraction (WAXD) were used to investigate the mechanisms of periodic assemblies leading to ring-banded crystal aggregates with light-grating capacity for iridescence in poly (1,4-butylene adipate) (PBA) modulated with poly (ethylene oxide) (PEO). A critical finding is that the PBA crystal assembly on the top surface and in the interior constitutes a grating architecture, with a cross-bar pitch equaling the inter-band spacing. The inner lamellae are arranged perpendicularly to the substrate under the ridge region, where they scroll, bend, and twist 90° to branch out newly spawned lamellae to form the parallel lamellae under the valley region. The cross-hatch grating with a fixed inter-spacing in the PBA aggregated crystals is proved in this work to perfectly act as light-interference entities capable of performing iridescence functions, which can be compared to those widely seen in many of nature’s organic bio-species or inorganic minerals such as opals. This is a novel breakthrough finding for PBA or similar polymers, such as photonic crystals, especially when the crystalline morphology could be custom-made and modulated with a second constituent.
Comparative Mechanical Study of Pressure Sensitive Adhesives over Aluminium Substrates for Industrial Applications
Authors: Ortega-Iguña Marta , Chludzinski Mariane , Sánchez-Amaya José María
Published: November 2022

The use of adhesives for fixing low-weight elements is showing increasing interest in the industry, as it would reduce the weight of the assembly, costs, and production time. Specifically, the application of pressure-sensitive adhesives (PSAs) to join non-structural naval components to aluminium substrates has not yet been reported. In the present work, a study of the mechanical behaviour of different double-sided PSAs applied on bare aluminium alloy substrates is performed. The influence of surface roughness, surface chemical treatments, and the matrix of the adhesives is studied through different mechanical tests, such as shear, T-peel, and creep. The application of an adhesion promoter improved the mechanical behaviour. Low roughness substrates provided better performance than ground samples. Acrylic foam adhesives were subjected to creep tests, whose results were fitted to a simple mathematical model, predicting the fracture time as a function of the applied load.
The Fabrication of Solid Polymer Electrolyte from CS/PEO/NaClO4/Fly Ash Composite
Authors: Ni’mah Yatim Lailun , Saputra Mohamat Ashar Eka , Suprapto Suprapto , Fansuri Hamzah , Suwarta Putu , Subhan Achmad , Pradanawati Sylvia Ayu
Published: November 2022

Solid polymer electrolytes (SPEs) have been successfully fabricated from CS/PEO/NaClO4/Fly ash composite. Chitosan (CS), an organic polymer, was blended with polyethylene oxide (PEO) to enhance its electrochemical properties. However, SPEs based on CS/PEO composites have low conductivity. Fly ash (FA) has been studied to be used as a filler to increase the ionic conductivity of SPEs. In this study, polymer composites based on CS and PEO were developed with the addition of FA as a filler using the solution casting method. The interactions between CS, PEO, NaClO4, and fly ash were observed using FTIR. The SPE characterization using XRD and DSC showed a decrease in crystallinity after the addition of NaClO4 and FA. The SPE composite morphology and elemental distribution were investigated using SEM. SPE conductivity analysis using EIS showed the optimum results for SPE fabricated with a ratio of CS:PEO:NaClO4 = 3:2:7.5, which was 1.02 × 10−4 S cm−1 at 30 °C and increased to 2.13 × 10−3 S cm−1 at 60 °C. The addition of FA (5 wt.%) increased the conductivity to 3.20 × 10−4 S cm−1 at 30 °C and increased to 4.34 × 10−3 S cm−1 at 60 °C.
Brief Review of PVDF Properties and Applications Potential
Authors: Dallaev Rashid , Pisarenko Tatiana , Sobola Dinara , Orudzhev Farid , Ramazanov Shikhgasan , Trčka Tomáš
Published: November 2022

Currently, there is an ever-growing interest in carbon materials with increased deformation-strength, thermophysical parameters. Due to their unique physical and chemical properties, such materials have a wide range of applications in various industries. Many prospects for the use of polymer composite materials based on polyvinylidene fluoride (PVDF) for scientific and technical purposes explain the plethora of studies on their characteristics “structure-property”, processing, application and ecology which keep appearing. Building a broader conceptual picture of new generation polymeric materials is feasible with the use of innovative technologies; thus, achieving a high level of multidisciplinarity and integration of polymer science; its fundamental problems are formed, the solution of which determines a significant contribution to the natural-scientific picture of the modern world. This review provides explanation of PVDF advanced properties and potential applications of this polymer material in its various forms. More specifically, this paper will go over PVDF trademarks presently available on the market, provide thorough overview of the current and potential applications. Last but not least, this article will also delve into the processing and chemical properties of PVDF such as radiation carbonization, β-phase formation, etc.
Using Hyperspectral Remote Sensing to Monitor Water Quality in Drinking Water Reservoirs
Authors: Goyens Clémence , Lavigne Héloïse , Dille Antoine , Vervaeren Han
Published: November 2022

At the Blankaart Water Production Center, a reservoir containing 3 million m3 of raw surface water acts as a first biologic treatment step before further processing to drinking water. Over the past decade, severe algal blooms have occurred in the reservoir, hampering the water production. Therefore, strategies (e.g., the injection of algaecide) have been looked at to prevent these from happening or try to control them. In this context, the HYperspectral Pointable System for Terrestrial and Aquatic Radiometry (HYPSTAR), installed since early 2021, helps in monitoring the effectiveness of these strategies. Indeed, the HYPSTAR provides, at a very high temporal resolution, bio-optical parameters related to the water quality, i.e., Chlorophyll-a (Chla) concentrations and suspended particulate matter (SPM). The present paper shows how the raw in situ hyperspectral data (a total of 8116 spectra recorded between 2021-02-03 and 2022-08-03, of which 2988 spectra passed the quality check) are processed to find the water-leaving reflectance and how SPM and Chla are derived from it. Based on a limited number of validation data, we also discuss the potential of retrieving phycocyanin (an accessory pigment unique to freshwater cyanobacteria). The results show the benefits of the high temporal resolution of the HYPSTAR to provide near real-time water quality indicators. The study confirms that, in conjunction with a few water sampling data used for validation, the HYPSTAR can be used as a quick and cost-effective method to detect and monitor phytoplankton blooms.
UAV-Based Multi-Temporal Thermal Imaging to Evaluate Wheat Drought Resistance in Different Deficit Irrigation Regimes
Authors: Qin Weilong , Wang Jian , Ma Longfei , Wang Falv , Hu Naiyue , Yang Xianyue , Xiao Yiyang , Zhang Yinghua , Sun Zhencai , Wang Zhimin , Yu Kang
Published: November 2022

Deficit irrigation is a common approach in water-scarce regions to balance productivity and water use, whereas drought stress still occurs to various extents, leading to reduced physiological performance and a decrease in yield. Therefore, seeking a rapid and reliable method to identify wheat varieties with drought resistance can help reduce yield loss under water deficit. In this study, we compared ten wheat varieties under three deficit irrigation systems (W0, no irrigation during the growing season; W1, irrigation at jointing; W2, irrigation at jointing and anthesis). UAV thermal imagery, plant physiological traits [leaf area index (LAI), SPAD, photosynthesis (Pn), transpiration (Tr), stomatal conductance (Cn)], biomass and yield were acquired at different growth stages. Wheat drought resistance performance was evaluated through using the canopy temperature extracted from UAV thermal imagery (CT-UAV), in combination with hierarchical cluster analysis (HCA). The CT-UAV of W0 and W1 treatments was significantly higher than in the W2 treatment, with the ranges of 24.8–33.3 °C, 24.3–31.6 °C, and 24.1–28.9 °C in W0, W1 and W2, respectively. We found negative correlations between CT-UAV and LAI, SPAD, Pn, Tr, Cn and biomass under the W0 (R2 = 0.41–0.79) and W1 treatments (R2 = 0.22–0.72), but little relevance for W2 treatment. Under the deficit irrigation treatments (W0 and W1), UAV thermal imagery was less effective before the grain-filling stage in evaluating drought resistance. This study demonstrates the potential of ensuring yield and saving irrigation water by identifying suitable wheat varieties for different water-scarce irrigation scenarios.
Remote Sensing of the Seasonal and Interannual Variability of Surface Chlorophyll-a Concentration in the Northwest Pacific over the Past 23 Years (1997–2020)
Authors: Chen Shuangling , Meng Yu , Lin Sheng , Xi Jingyuan
Published: November 2022

Phytoplankton in the northwest Pacific plays an important role in absorbing atmospheric CO2 and promoting the ocean carbon cycle. However, our knowledge on the long-term interannual variabilities of the phytoplankton biomass in this region is quite limited. In this study, based on the Chlorophyll-a concentration (Chl-a) time series observed from ocean color satellites of Sea-viewing Wide Field-of-view Sensor (SeaWiFS) and Moderate Resolution Imaging Spectroradiometer (MODIS) in the period of 1997–2020, we investigated the variabilities of Chl-a on both seasonal and interannual scales, as well as the long-term trends. The phytoplankton Chl-a showed large spatial dynamics with a general decreasing pattern poleward. The seasonal phytoplankton blooms dominated the seasonal characteristics of Chl-a, with spring and fall blooms identified in subpolar waters and single spring blooms in subtropical seas. On interannual scales, we found a Chl-a increasing belt in the subpolar oceans from the marginal sea toward the northeast open ocean waters, with positive trends (~0.02 mg m−3 yr−1, on average) in Chl-a at significant levels (p < 0.05). In the subtropical gyre, Chl-a showed slight but significant negative trends (i.e., <−0.0006 mg m−3 yr−1, at p < 0.05). The negative Chl-a trends in the subtropical waters tended to be driven by the surface warming, which could inhibit nutrient supplies from the subsurface and thus limit phytoplankton growth. For the subpolar waters, although the surface warming also prevailed over the study period, the in situ surface nitrate reservoir somehow showed significant increases in the targeted spots, indicating potential external nitrate supplies into the surface layer. We did not find significant connections between the Chl-a interannual variabilities and the climate indices in the study area. Environmental data with finer spatial and temporal resolutions will further constrain the findings.
Comparison on Quantitative Analysis of Olivine Using MarSCoDe Laser-Induced Breakdown Spectroscopy in a Simulated Martian Atmosphere
Authors: Liu Xiangfeng , Xu Weiming , Li Luning , Xu Xuesen , Qi Hai , Zhang Zhenqiang , Yang Fan , Yan Zhixin , Liu Chongfei , Yuan Rujun , Wan Xiong , Shu Rong
Published: November 2022

A Mars Surface Composition Detector (MarSCoDe) instrument mounted on Zhurong rover of Tianwen-1, adopts Laser-Induced Breakdown Spectroscopy (LIBS), with no sample preparation or dust and coatings ablation required, to conduct rapid multi-elemental analysis and characterization of minerals, rocks and soils on the surface of Mars. To test the capability of MarSCoDe LIBS measurement and quantitative analysis, some methods of multivariate analysis on olivine samples with gradient concentrations were inspected based on the spectra acquired in a Mars-simulated environment before the rover launch in 2020. Firstly, LIBS spectra need preprocessing, including background subtraction, random signal denoising, continuum baseline removal, spectral drift correction and wavelength calibration, radiation calibration, and multi-channel spectra subset merging. Then, the quantitative analysis with univariate linear regression (ULR) and multivariate linear regression (MLR) are performed on the characteristic lines, while principal component regression (PCR), partial least square regression (PLSR), ridge, least-absolute-shrinkage-and-selection-operator (LASSO) and elastic net, and nonlinear analysis with back-propagation (BP) are conducted on the entire spectral information. Finally, the performance on the quantitative olivine analyzed by MarSCoDe LIBS is compared with the mean spectrum and all spectra for each sample and evaluated by some statistical indicators. The results show that: (1) the calibration curve of ULR constructed by the characteristic line of magnesium and iron indicates the linear relationship between the spectral signal and the element concentration, and the limits of detection of forsterite and fayalite is 0.9943 and 2.0536 (c%) analyzed by mean spectra, and 2.3354 and 3.8883 (c%) analyzed by all spectra; (2) the R2 value on the calibration and validation of all the methods is close to 1, and the predicted concentration estimated by these calibration models is close to the true concentration; (3) the shrinkage or regularization technique of ridge, LASSO and elastic net perform better than the ULR and MLR, except for ridge overfitting on the testing sample; the best results can be obtained by the dimension reduction technique of PCR and PLSR, especially with PLSR; and BP is more applicable for the sample measured with larger spectral dataset.