bluuebunny/crossref_metadata_2025 huggingface dataset:
Multi-Criteria Method for the Selection of Renewable Energy Sources in the Polish Industrial Sector
Authors: Ulewicz Robert , Siwiec Dominika , Pacana Andrzej , Tutak Magdalena , Brodny Jarosław
Published: April 2021

Rational decision-making requires assessing the advantages and disadvantages of options, including nonmarket effects (such as environmental effects). This also applies to strategic decision-making in the industrial sector to select alternative renewable energy source (RES). Often, a variety of criteria can be used to select a renewable energy source, whereas no ideal family of criteria for renewable energy selection for industry has been defined in the literature. It was concluded that there is a need to support the actions of industrial development based on RES, which will contribute significantly to overcoming the limitations of the negative effect on the environment in terms of greenhouse gas emissions. There is a clear need for a systematic and polyvalent multicriteria approach to planning in industry. Therefore, a method for choosing the preferred renewable source of electricity for industry has been developed, which considers key criteria of RES choice: Expert opinions, the costs of obtaining the energy and maintaining energy installations, and the volume of electricity from RES. This article offers a modified multicriteria selection method based on a fuzzy analytic hierarchy process (fuzzy AHP) and the technique for preference by similarity to an ideal solution (TOPSIS), integrated with a qualitative price analysis (ACJ). This new method was tested through a case study on selecting a preferred RES in Polish industrial conditions. The research results indicate that the proposed method of choosing the preferred renewable energy source can be used in industrial enterprises that strive to meet their energy needs in accordance with the principles of social responsibility.
Associations of Phoretic Mites on Bark Beetles of the Genus Ips in the Black Sea Mountains of Turkey
Authors: Cilbircioğlu Cihan , Kovač Marta , Pernek Milan
Published: April 2021

Phoretic mites use bark beetles for transportation to new, suitable habitats. Some phoretic mites act as predators and parasitoids of the bark beetles’ immature stages, especially egg and early larval stages, and are potential agents for the biological control of scolytine forest pests. Mites live very frequently in relationships with other invertebrates. Many are found in association with various species of bark beetles. Here, a total of 41 specimens of different bark beetles of the genus Ips (Ips acuminatus, Ips sexdentatus and Ips typographus) were studied for presence, species composition, and abundance of phoretic mites. The beetles were collected on dead wood and parts of tree bark of Pinus nigra, Pinus sylvestris and Picea abies in the Black Sea Mountains in Kastamonu and Artin Province of Turkey. A total of nine mite species were found, including Dendrolaelaps quadrisetus, Ereynetes sp., Histiostoma piceae, Paraleius cf. leontonychus, Pleuronectocelaeno barbara., Proctolaelaps hystricoides, Schizostethus simulatrix, Trichouropoda lamellosa and Uroobovellaipidis. All species are identified for the first time within Turkish fauna.
Application of Date (Phoenix dactylifera L.) Fruit in the Composition of a Novel Snack Bar
Authors: Ibrahim Salam A. , Fidan Hafize , Aljaloud Sulaiman O. , Stankov Stanko , Ivanov Galin
Published: April 2021

The aim of the present study was to evaluate the possibilities for using date fruit from the Kingdom of Saudi Arabia in the formulation of a novel snack bar while replacing the used honey with date paste. The technological, textural, microbiological and sensory qualities of the obtained food products were evaluated during storage for 12 days after their production. Date palm fruit in the form of date paste was used as an ingredient in the composition of the new snack bars that also included nuts and dried fruits. Five formulations were prepared: a control bar, snack bar with 40% date paste, snack bar with 50% date paste, snack bar with 60% date paste, and snack bar with 70% dates paste. The resulting date paste’s textural characteristics supported the bonding potential of the food system and gave a sweet taste to the final product. The formulations containing 50% date paste presented the highest overall acceptability and were the formulation with the best sensory characteristics. Thus, the addition of date paste in snack bars would be a good option to develop a functional product that contributes to rational nutrition principles. The obtained values in the current study confirmed the technological and functional potential of date fruit as a product that can find adequate application in the composition of foods with functional properties.
Traditional and Non-Conventional Pasta-Making Processes: Effect on In Vitro Starch Digestibility
Authors: Dodi Rossella , Bresciani Letizia , Biasini Beatrice , Cossu Marta , Scazzina Francesca , Taddei Federica , D’Egidio Maria Grazia , Dall’Asta Margherita , Martini Daniela
Published: April 2021

Pasta is a carbohydrate-rich food with a low glycemic index (GI) and is one of the main sources of slowly digestible starch (SDS). The presence of bran fractions (BFs) in pasta may enhance its health potential, owing to the content of fiber, micronutrients, and bioactive compounds; however, at the same time, BF may affect starch digestibility. In this study, the bioaccessibility of starch in pasta made with BF-enriched semolina (BF pasta), or only with micronized debranned kernel (DK pasta), and a control pasta made with traditional semolina was evaluated by applying two different in vitro models. The control pasta showed a percentage of SDS about four-fold higher than that of the BF pasta and 1.5-fold higher than that of the DK pasta (p < 0.05). The amount of starch released during simulated gastrointestinal digestion was slightly lower, but not significantly different, for the control pasta than for both the BF and DK pasta. These results suggest that the presence of a higher amount of dietary fiber in BF pasta can affect the structure of the food matrix, interfering with the formation of the gluten network, water absorption, and starch granule accessibility, while micronization could enhance starch digestibility due to starch gelatinization. These findings emphasize the need to optimize the process for producing fiber-rich pasta without affecting its low starch digestibility and, consequently, its GI.
Down-Regulation of SlGRAS10 in Tomato Confers Abiotic Stress Tolerance
Authors: Habib Sidra , Lwin Yee Yee , Li Ning
Published: April 2021

Adverse environmental factors like salt stress, drought, and extreme temperatures, cause damage to plant growth, development, and crop yield. GRAS transcription factors (TFs) have numerous functions in biological processes. Some studies have reported that the GRAS protein family plays significant functions in plant growth and development under abiotic stresses. In this study, we demonstrated the functional characterization of a tomato SlGRAS10 gene under abiotic stresses such as salt stress and drought. Down-regulation of SlGRAS10 by RNA interference (RNAi) produced dwarf plants with smaller leaves, internode lengths, and enhanced flavonoid accumulation. We studied the effects of abiotic stresses on RNAi and wild-type (WT) plants. Moreover, SlGRAS10-RNAi plants were more tolerant to abiotic stresses (salt, drought, and Abscisic acid) than the WT plants. Down-regulation of SlGRAS10 significantly enhanced the expressions of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) to reduce the effects of reactive oxygen species (ROS) such as O2− and H2O2. Malondialdehyde (MDA) and proline contents were remarkably high in SlGRAS10-RNAi plants. Furthermore, the expression levels of chlorophyll biosynthesis, flavonoid biosynthesis, and stress-related genes were also enhanced under abiotic stress conditions. Collectively, our conclusions emphasized the significant function of SlGRAS10 as a stress tolerate transcription factor in a certain variety of abiotic stress tolerance by enhancing osmotic potential, flavonoid biosynthesis, and ROS scavenging system in the tomato plant.
Molecular Cloning and Functional Analysis of 1-Deoxy-D-Xylulose 5-Phosphate Reductoisomerase from Santalum album
Authors: Zhang Yueya , Yan Haifeng , Li Yuan , Xiong Yuping , Niu Meiyun , Zhang Xinhua , Teixeira da Silva Jaime A. , Ma Guohua
Published: April 2021

Sandalwood (Santalum album L.) heartwood-derived essential oil contains a high content of sesquiterpenoids that are economically highly valued and widely used in the fragrance industry. Sesquiterpenoids are biosynthesized via the mevalonate acid and methylerythritol phosphate (MEP) pathways, which are also the sources of precursors for photosynthetic pigments. 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) is a secondary rate-limiting enzyme in the MEP pathway. In this paper, the 1416-bp open reading frame of SaDXR and its 897-bp promoter region, which contains putative conserved cis-elements involved in stress responsiveness (HSE and TC-rich repeats), hormone signaling (abscisic acid, gibberellin and salicylic acid) and light responsiveness, were cloned from 7-year-old S. album trees. A bioinformatics analysis suggested that SaDXR encodes a functional and conserved DXR protein. SaDXR was widely expressed in multiple tissues, including roots, twigs, stem sapwood, leaves, flowers, fruit and stem heartwood, displaying significantly higher levels in tissues with photosynthetic pigments, like twigs, leaves and flowers. SaDXR mRNA expression increased in etiolated seedlings exposed to light, and the content of chlorophylls and carotenoids was enhanced in all 35S::SaDXR transgenic Arabidopsis thaliana lines, consistent with the SaDXR expression level. SaDXR was also stimulated by MeJA and H2O2 in seedling roots. α-Santalol content decreased in response to fosmidomycin, a DXR inhibitor. These results suggest that SaDXR plays an important role in the biosynthesis of photosynthetic pigments, shifting the flux to sandalwood-specific sesquiterpenoids.
Inversion Table Fall Injury, the Phantom Menace: Three Case Reports on Cervical Spinal Cord Injury
Authors: Jung Seung-Hwan , Hwang Jong-Moon , Kim Chul-Hyun
Published: April 2021

Background: An inversion device, which is used to suspend one’s body and perform traction therapy, was introduced as an inversion table under the name of “Geokkuri” in South Korea. Fall injuries while hanging on inversion tables are among the most devastating spine injuries, as the likelihood of severe neurological sequelae such as tetraplegia increases. However, its enormous danger has been overlooked and this devastating injury has become a common clinical entity over time. The limited number of studies reported imply the lack of interest of researchers in these injuries. We reviewed three cases of spinal cord injury sustained on inversion tables in different environments and report the potential danger associated with the use of inversion tables to facilitate a safer exercise environment.
Empathy and Coping in Allied Health Sciences: Gender Patterns
Authors: Dores Artemisa R. , Martins Helena , Reis Ana C. , Carvalho Irene P.
Published: April 2021

This study aimed to examine the patterns of associations between empathy and coping among undergraduate men and women studying at Allied Health Sciences. This cross-sectional study is part of a larger longitudinal study conducted in an Allied Health Sciences School. Participants were 183 undergraduate students from 12 training programs (e.g., Physiotherapy, Occupational Therapy, Speech Therapy). Their mean age was 20.79 years (SD = 2.64), and they were in their first, third, and fourth years of school. The instruments were the Brief-COPE and the Interpersonal Reactivity Index (IRI). Empathy correlated with coping strategies in both genders, though showing different patterns of association. First, distinct coping strategies were associated with the same empathy dimension (perspective taking) among women (positive reframing and self-blame) and among men (active coping). Second, the same three coping strategies appeared in both genders (seeking emotional or instrumental support and resorting to religion) but associated with different empathy dimensions (cognitive empathy among women and mostly emotional empathy among men). Third, among women (but not among men), two coping strategies (positive reframing and behavioral disengagement) were each simultaneously correlated with cognitive and emotional empathy in opposite directions. Fourth, emotional empathy correlated, only among women, with several coping strategies considered to be maladaptive (behavioral disengagement, denial and substance use). Among men, only one significant coping strategy was considered to be maladaptive (behavioral disengagement) and it was negatively correlated with cognitive empathy (perspective taking). Unlike in women, relationships between the empathic dimension of fantasy and coping strategies were non-significant among men. These distinct patterns of associations emerged despite significant differences in empathy by gender (fantasy, personal distress and empathic concern) and in coping strategies (instrumental support, emotional support, religion and venting). These results support the idea that the display of empathy might be associated with gender differences in the underlying empathy dimensions and in the coping strategies used to deal with stress in the undergraduate programs of Allied Health Sciences.
Prevalence and Course of IgA and IgG Antibodies against SARS-CoV-2 in Healthcare Workers during the First Wave of the COVID-19 Outbreak in Germany: Interim Results from an Ongoing Observational Cohort Study
Authors: Reinwald Mark , Deckert Peter Markus , Ritter Oliver , Andresen Henrike , Schreyer Andreas G. , Weylandt Karsten Henrich , Dammermann Werner , Lüth Stefan
Published: April 2021

(1) Background: Healthcare workers (HCWs) are prone to intensified exposure to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection in the ongoing pandemic. We prospectively analyzed the prevalence of antibodies against SARS-CoV-2 in HCWs at baseline and follow up with regard to clinical signs and symptoms in two university hospitals in Brandenburg, Germany. (2) Methods: Screening for anti-SARS-CoV-2 IgA and IgG antibodies was offered to HCWs at baseline and follow up two months thereafter in two hospitals of Brandenburg Medical School during the first wave of the COVID-19 pandemic in Germany in an ongoing observational cohort study. Medical history and signs and symptoms were recorded by questionnaires and analyzed. (3) Results: Baseline seroprevalence of anti-SARS-CoV-2 IgA was 11.7% and increased to 15% at follow up, whereas IgG seropositivity was 2.1% at baseline and 2.2% at follow up. The rate of asymptomatic seropositive cases was 39.5%. Symptoms were not associated with general seropositivity for anti-SARS-CoV-2; however, class switch from IgA to IgG was associated with increased symptom burden. (4) Conclusions: The seroprevalence of antibodies against SARS-CoV-2 was low in HCWs but higher compared to population data and increased over time. Screening for antibodies detected a significant proportion of seropositive participants cases without symptoms.
Dimensions Used in Instruments for QALY Calculation: A Systematic Review
Authors: Touré Moustapha , Kouakou Christian R. C. , Poder Thomas G.
Published: April 2021

Economic assessment is of utmost importance in the healthcare decision-making process. The quality-adjusted life-year (QALY) concept provides a rare opportunity to combine two crucial aspects of health, i.e., mortality and morbidity, into a single index to perform cost-utility comparison. Today, many tools are available to measure morbidity in terms of health-related quality of life (HRQoL) and a large literature describes how to use them. Knowing their characteristics and development process is a key point for elaborating, adapting, or selecting the most well-suited instrument for further needs. In this aim, we conducted a systematic review on instruments used for QALY calculation, and 46 studies were selected after searches in four databases: Medline EBSCO, Scopus, ScienceDirect, and PubMed. The search procedure was done to identify all relevant publications up to 18 June 2020. We mainly focused on the type of instrument developed (i.e., generic or specific), the number and the nature of dimensions and levels used, the elicitation method and the model selected to determine utility scores, and the instrument and algorithm validation methods. Results show that studies dealing with the development of specific instruments were mostly motivated by the inappropriateness of generic instruments in their field. For the dimensions’ and levels’ selection, item response theory, Rasch analysis, and literature review were mostly used. Dimensions and levels were validated by methods like the Loevinger H, the standardised response mean, or discussions with experts in the field. The time trade-off method was the most widely used elicitation method, followed by the visual analogue scale. Random effects regression models were frequently used in determining utility scores.
Expected Scopes of Health Emergency and Disaster Risk Management (Health EDRM): Report on the Expert Workshop at the Annual Conference for the Japanese Association for Disaster Medicine 2020
Authors: Nomura Shuhei , Kayano Ryoma , Egawa Shinichi , Harada Nahoko , Koido Yuichi
Published: April 2021

The World Health Organization (WHO) and its partners established the WHO Thematic Platform for Health Emergency and Disaster Risk Management Research Network (HEALTH EDRM RN) in 2016 to respond to the increasing burden of recent health emergencies and disasters. The mission of the HEALTH EDRM RN, whose secretariat is located at the WHO Kobe Centre (WKC), is to promote global research collaboration and strengthen research activities to inform policies and programs by generating new evidence to manage health risks associated with all types of emergencies and disasters. With the strong support and involvement of all WHO regional offices, the HEALTH EDRM RN now works with more than 200 global experts and partners to pursue its mission. The first Core Group Meetings of the HEALTH EDRM RN were held on 17–18 October 2019, and concluded with the HEALTH EDRM RN-activity priorities to (1) promote operational research to better meet the needs of emergency- and disaster-exposed individuals and communities and efforts to translate science to policies and programs and (2) strengthen the research capacity of the Health EDRM community. In collaboration with the Japanese Association for Disaster Medicine, the WKC held a workshop on 21 February 2020, in which 20 Japanese experts from different research fields participated to further discuss these two points. This paper summarizes the discussion at the workshop.
Radon Adsorption in Charcoal
Authors: Maier Andreas , Jones Jesse , Sternkopf Sonja , Friedrich Erik , Fournier Claudia , Kraft Gerhard
Published: April 2021

Radon is pervasive in our environment and the second leading cause of lung cancer induction after smoking. Therefore, the measurement of radon activity concentrations in homes is important. The use of charcoal is an easy and cost-efficient method for this purpose, as radon can bind to charcoal via Van der Waals interaction. Admittedly, there are potential influencing factors during exposure that can distort the results and need to be investigated. Consequently, charcoal was exposed in a radon chamber at different parameters. Afterward, the activity of the radon decay products 214Pb and 214Bi was measured and extrapolated to the initial radon activity in the sample. After an exposure of 1 h, around 94% of the maximum value was attained and used as a limit for the subsequent exposure time. Charcoal was exposed at differing humidity ranging from 5 to 94%, but no influence on radon adsorption could be detected. If the samples were not sealed after exposure, radon desorbed with an effective half-life of around 31 h. There is also a strong dependence of radon uptake on the chemical structure of the recipient material, which is interesting for biological materials or diffusion barriers as this determines accumulation and transport.
Will Households Invest in Safe Sanitation? Results from an Experimental Demand Trial in Nakuru, Kenya
Authors: Peletz Rachel , Delaire Caroline , Kones Joan , MacLeod Clara , Samuel Edinah , Easthope-Frazer Alicea , Khush Ranjiv
Published: April 2021

Unsafe sanitation is an increasing public health concern for rapidly expanding cities in low-income countries. Understanding household demand for improved sanitation infrastructure is critical for planning effective sanitation investments. In this study, we compared the stated and revealed willingness to pay (WTP) for high-quality, pour-flush latrines among households in low-income areas in the city of Nakuru, Kenya. We found that stated WTP for high-quality, pour-flush latrines was much lower than market prices: less than 5% of households were willing to pay the full costs, which we estimated between 87,100–82,900 Kenyan Shillings (KES), or 871–829 USD. In addition, we found large discrepancies between stated and revealed WTP. For example, 90% of households stated that they would be willing to pay a discounted amount of 10,000 KES (100 USD) for a high-quality, pour-flush latrine, but only 10% of households redeemed vouchers at this price point (paid via six installment payments). Households reported that financial constraints (i.e., lack of cash, other spending priorities) were the main barriers to voucher redemption, even at highly discounted prices. Our results emphasize the importance of financial interventions that address the sizable gaps between the costs of sanitation products and customer demand among low-income populations.
College Classroom Instructors Can Effectively Promote Standing among Students Provided with Standing Desks
Authors: Chrisman Matthew S. , Wright Robert , Purdy William
Published: April 2021

Standing desks may reduce sedentary behaviors in college students. Students at one mid-size urban university in the Midwestern United States were randomized into intervention (n = 21) and control groups (n = 27) to assess standing time when given access to standing desks. The intervention group received visual and oral instructor prompts to stand, while the control received no prompts during a 50 min lecture. All students were provided with adjustable tabletop standing desks. ActivPAL accelerometers measured sitting and standing time. A brief survey assessed student preferences, including facilitators and barriers to standing. Mean standing time was greater in the intervention vs. control group (26 vs. 17 min, p = 0.023). Students tended to stand in the corners and edges of the room. Main facilitators for standing included to break up sitting, reduce back pain, and increase attention and focus; main barriers were not wanting to distract others or be the only one standing. In total, 87.5% of intervention group participants found five prompts to stand were adequate. Students increased standing time in class when provided with standing desks and instructor prompts to stand. Findings can inform the layout of classrooms and when and how to promote standing desks during lectures.
Deubiquitinases: Modulators of Different Types of Regulated Cell Death
Authors: Lee Choong-Sil , Kim Seungyeon , Hwang Gyuho , Song Jaewhan
Published: April 2021

The mechanisms and physiological implications of regulated cell death (RCD) have been extensively studied. Among the regulatory mechanisms of RCD, ubiquitination and deubiquitination enable post-translational regulation of signaling by modulating substrate degradation and signal transduction. Deubiquitinases (DUBs) are involved in diverse molecular pathways of RCD. Some DUBs modulate multiple modalities of RCD by regulating various substrates and are powerful regulators of cell fate. However, the therapeutic targeting of DUB is limited, as the physiological consequences of modulating DUBs cannot be predicted. In this review, the mechanisms of DUBs that regulate multiple types of RCD are summarized. This comprehensive summary aims to improve our understanding of the complex DUB/RCD regulatory axis comprising various molecular mechanisms for diverse physiological processes. Additionally, this review will enable the understanding of the advantages of therapeutic targeting of DUBs and developing strategies to overcome the side effects associated with the therapeutic applications of DUB modulators.
Emerging Role of Phospholipase-Derived Cleavage Products in Regulating Eosinophil Activity: Focus on Lysophospholipids, Polyunsaturated Fatty Acids and Eicosanoids
Authors: Knuplez Eva , Sturm Eva Maria , Marsche Gunther
Published: April 2021

Eosinophils are important effector cells involved in allergic inflammation. When stimulated, eosinophils release a variety of mediators initiating, propagating, and maintaining local inflammation. Both, the activity and concentration of secreted and cytosolic phospholipases (PLAs) are increased in allergic inflammation, promoting the cleavage of phospholipids and thus the production of reactive lipid mediators. Eosinophils express high levels of secreted phospholipase A2 compared to other leukocytes, indicating their direct involvement in the production of lipid mediators during allergic inflammation. On the other side, eosinophils have also been recognized as crucial mediators with regulatory and homeostatic roles in local immunity and repair. Thus, targeting the complex network of lipid mediators offer a unique opportunity to target the over-activation and ‘pro-inflammatory’ phenotype of eosinophils without compromising the survival and functions of tissue-resident and homeostatic eosinophils. Here we provide a comprehensive overview of the critical role of phospholipase-derived lipid mediators in modulating eosinophil activity in health and disease. We focus on lysophospholipids, polyunsaturated fatty acids, and eicosanoids with exciting new perspectives for future drug development.
The Inflammatory Role of Pro-Resolving Mediators in Endometriosis: An Integrative Review
Authors: de Fáveri Cássia , Fermino Paula M. Poeta , Piovezan Anna P. , Volpato Lia K.
Published: April 2021

The pathogenesis of endometriosis is still controversial, although it is known that the inflammatory immune response plays a critical role in this process. The resolution of inflammation is an active process where the activation of endogenous factors allows the host tissue to maintain homeostasis. The mechanisms by which pro-resolving mediators (PRM) act in endometriosis are still little explored. Thus, this integrative review aims to synthesize the available content regarding the role of PRM in endometriosis. Experimental and in vitro studies with Lipoxin A4 demonstrate a potential inhibitory effect on endometrial lesions’ progression, attenuating pro-inflammatory and angiogenic signals, inhibiting proliferative and invasive action suppressing intracellular signaling induced by cytokines and estradiol, mainly through the FPR2/ALX. Investigations with Resolvin D1 demonstrated the inhibition of endometrial lesions and decreased pro-inflammatory factors. Annexin A1 is expressed in the endometrium and is specifically present in women with endometriosis, although the available studies are still inconsistent. Thus, we believe there is a gap in knowledge regarding the PRM pathways in patients with endometriosis. It is important to note that these substances’ therapeutic potential is evident since the immune and abnormal inflammatory responses play an essential role in endometriosis development and progression.
Resveratrol Alleviates the Inhibitory Effect of Tunicamycin-Induced Endoplasmic Reticulum Stress on Expression of Genes Involved in Thyroid Hormone Synthesis in FRTL-5 Thyrocytes
Authors: Wen Gaiping , Eder Klaus , Ringseis Robert
Published: April 2021

Recently, ER stress induced by tunicamycin (TM) was reported to inhibit the expression of key genes involved in thyroid hormone synthesis, such as sodium/iodide symporter (NIS), thyroid peroxidase (TPO) and thyroglobulin (TG), and their regulators such as thyrotropin receptor (TSHR), thyroid transcription factor-1 (TTF-1), thyroid transcription factor-2 (TTF-2) and paired box gene 8 (PAX-8), in FRTL-5 thyrocytes. The present study tested the hypothesis that resveratrol (RSV) alleviates this effect of TM in FRTL-5 cells. While treatment of FRTL-5 cells with TM alone (0.1 µg/mL) for 48 h strongly induced the ER stress-sensitive genes heat shock protein family A member 5 (HSPA5) and DNA damage inducible transcript 3 (DDIT3) and repressed NIS, TPO, TG, TSHR, TTF-1, TTF-2 and PAX-8, combined treatment with TM (0.1 µg/mL) and RSV (10 µM) for 48 h attenuated this effect of TM. In conclusion, RSV alleviates TM-induced ER stress and attenuates the strong impairment of expression of genes involved in thyroid hormone synthesis and their regulators in FRTL-5 thyrocytes exposed to TM-induced ER stress. Thus, RSV may be useful for the treatment of specific thyroid disorders, provided that strategies with improved oral bioavailability of RSV are applied.
Biomarkers in Glycogen Storage Diseases: An Update
Authors: Molares-Vila Alberto , Corbalán-Rivas Alberte , Carnero-Gregorio Miguel , González-Cespón José Luís , Rodríguez-Cerdeira Carmen
Published: April 2021

Glycogen storage diseases (GSDs) are a group of 19 hereditary diseases caused by a lack of one or more enzymes involved in the synthesis or degradation of glycogen and are characterized by deposits or abnormal types of glycogen in tissues. Their frequency is very low and they are considered rare diseases. Except for X-linked type IX, the different types are inherited in an autosomal recessive pattern. In this study we reviewed the literature from 1977 to 2020 concerning GSDs, biomarkers, and metabolic imbalances in the symptoms of some GSDs. Most of the reported studies were performed with very few patients. Classification of emerging biomarkers between different types of diseases (hepatics GSDs, McArdle and PDs and other possible biomarkers) was done for better understanding. Calprotectin for hepatics GSDs and urinary glucose tetrasaccharide for Pompe disease have been approved for clinical use, and most of the markers mentioned in this review only need clinical validation, as a final step for their routine use. Most of the possible biomarkers are implied in hepatocellular adenomas, cardiomyopathies, in malfunction of skeletal muscle, in growth retardation, neutropenia, osteopenia and bowel inflammation. However, a few markers have lost interest due to a great variability of results, which is the case of biotinidase, actin alpha 2, smooth muscle, aorta and fibroblast growth factor receptor 4. This is the first review published on emerging biomarkers with a potential application to GSDs.
Small Bowel Epithelial Precursor Lesions: A Focus on Molecular Alterations
Authors: Vanoli Alessandro , Grillo Federica , Furlan Daniela , Arpa Giovanni , Grami Oneda , Guerini Camilla , Riboni Roberta , Mastracci Luca , Di Sabatino Antonio
Published: April 2021

The wider use of gastrointestinal endoscopic procedures has led to an increased detection of small intestinal preneoplastic and neoplastic epithelial lesions, most of which are identified in the duodenum and ampullary region. Like their malignant counterparts, small intestinal glandular precursor lesions, which include adenomas and hamartomas, may arise sporadically or be associated with hereditary tumor syndromes, such as familial adenomatous polyposis, MUTYH-associated polyposis, Lynch syndrome, Peutz-Jeghers syndrome, juvenile polyposis syndrome, and Cowden syndrome. In addition, dysplastic, preinvasive lesions have been observed adjacent to small bowel adenocarcinomas complicating immune-related disorders, such as celiac or Crohn’s disease. Adenomatous lesions may exhibit an intestinal-type, gastric-type, or, very rarely, serrated differentiation, related to different molecular pathogenetic mechanisms. Finally, in the background of multiple endocrine neoplasia 1 syndrome, precursor neuroendocrine growths have been described. In this review we offer a comprehensive description on the histo-molecular features of the main histotypes of small bowel epithelial precursors lesions, including: (i) sporadic adenomas (intestinal-type and gastric-type; non-ampullary and ampullary); (ii) syndromic adenomas; (iii) small bowel dysplasia in celiac and Crohn’s disease; (iv) serrated lesions; (v) hamartomatous lesions; and (vi) neuroendocrine precursor lesions.
Sexually Dimorphic Immune and Neuroimmune Changes Following Peripheral Nerve Injury in Mice: Novel Insights for Gender Medicine
Authors: Vacca Valentina , Marinelli Sara , De Angelis Federica , Angelini Daniela F. , Piras Eleonora , Battistini Luca , Pavone Flaminia , Coccurello Roberto
Published: April 2021

Neuropathic pain (NeP) in humans is often a life-long condition with no effective therapy available. The higher incidence of female gender in NeP onset is worldwide reported, and although the cause is generally attributed to sex hormones, the actual mechanisms and the players involved are still unclear. Glial and immune cells take part in NeP development, and orchestrate the neuroimmune and inflammatory response, releasing pro-inflammatory factors with chemoattractant properties that activate resident immune cells and recruit immune cells from circulation. The neuro-immune crosstalk is a key contributor to pain hypersensitivity following peripheral nervous system injury. Our previous works showed that in spite of the fact that female mice had an earlier analgesic response than males following nerve lesion, the recovery from NeP was never complete, suggesting that this difference could occur in the very early stages after injury. To further investigate gender differences in immune and neuroimmune responses to NeP, we studied the main immune cells and mediators elicited both in plasma and sciatic nerves by peripheral nerve lesion. After injury, we found a different pattern of distribution of immune cell populations showing either a higher infiltration of T cells in nerves from females or a higher infiltration of macrophages in nerves from males. Moreover, in comparison to male mice, the levels of cytokines and chemokines were differently up- and down-regulated in blood and nerve lysates from female mice. Our study provides some novel insights for the understanding of gender-associated differences in the generation and perseveration of NeP as well as for the isolation of specific neurodegenerative mechanisms underlying NeP. The identification of gender-associated inflammatory profiles in neuropathy is of key importance for the development of differential biomarkers and gender-specific personalized medicine.
Evaluation of Numerical Simulation Approaches for Simulating Train–Track Interactions and Predicting Rail Damage in Railway Switches and Crossings (S&Cs)
Authors: Pillai Nikhil , Shih Jou-Yi , Roberts Clive
Published: April 2021

Switch and crossing (S&C) faults are a major cause of track-related delays and account for a significant proportion of maintenance and renewal budgets for railway infrastructure managers around the world. Although various modelling approaches have been proposed in the literature for the simulation of vehicle–track dynamic interaction, wheel–rail contact and damage prediction, there is a lack of evaluation for combining these approaches to effectively predict the failure mechanism. An evaluation of S&C modelling approaches has therefore been performed in this article to justify their selection for the research interests of predicting the most dominant failure mechanisms of wear, rolling contact fatigue (RCF) and plastic deformation in S&C rails by recognising the factors that influence the accuracy and efficiency of the proposed modelling approaches. A detailed discussion of the important modelling aspects has been carried out by considering the effectiveness of each individual approach and the combination of different approaches, along with a suggestion of appropriate modelling approaches for predicting the dominant failure mechanisms.
Contrasting Manual and Automated Assessment of Thermal Stress Responses and Larval Body Size in Black Soldier Flies and Houseflies
Authors: Laursen Stine Frey , Hansen Laura Skrubbeltrang , Bahrndorff Simon , Nielsen Hanne Marie , Noer Natasja Krog , Renault David , Sahana Goutam , Sørensen Jesper Givskov , Kristensen Torsten Nygaard
Published: April 2021

Within ecophysiological and genetic studies on insects, morphological and physiological traits are commonly assessed and phenotypes are typically obtained from manual measurements on numerous individuals. Manual observations are, however, time consuming, can introduce observer bias and are prone to human error. Here, we contrast results obtained from manual assessment of larval size and thermal tolerance traits in black soldier flies (Hermetia illucens) and houseflies (Musca domestica) that have been acclimated under three different temperature regimes with those obtained automatically using an image analysis software (Noldus EthoVision XT). We found that (i) larval size estimates of both species, obtained by manual weighing or by using the software, were highly correlated, (ii) measures of heat and cold tolerance using manual and automated approaches provided qualitatively similar results, and (iii) by using the software we obtained quantifiable information on stress responses and acclimation effects of potentially higher ecological relevance than the endpoint traits that are typically assessed when manual assessments are used. Based on these findings, we argue that automated assessment of insect stress responses and largescale phenotyping of morphological traits such as size will provide new opportunities within many disciplines where accurate and largescale phenotyping of insects is required.
A 0.3 V Rail-to-Rail Ultra-Low-Power OTA with Improved Bandwidth and Slew Rate
Authors: Centurelli Francesco , Della Sala Riccardo , Monsurrò Pietro , Scotti Giuseppe , Trifiletti Alessandro
Published: April 2021

In this paper, we present a novel operational transconductance amplifier (OTA) topology based on a dual-path body-driven input stage that exploits a body-driven current mirror-active load and targets ultra-low-power (ULP) and ultra-low-voltage (ULV) applications, such as IoT or biomedical devices. The proposed OTA exhibits only one high-impedance node, and can therefore be compensated at the output stage, thus not requiring Miller compensation. The input stage ensures rail-to-rail input common-mode range, whereas the gate-driven output stage ensures both a high open-loop gain and an enhanced slew rate. The proposed amplifier was designed in an STMicroelectronics 130 nm CMOS process with a nominal supply voltage of only 0.3 V, and it achieved very good values for both the small-signal and large-signal Figures of Merit. Extensive PVT (process, supply voltage, and temperature) and mismatch simulations are reported to prove the robustness of the proposed amplifier.
Environmental Characteristics and Macrobenthic Community Structure of Yubu Island, a UNESCO-Designated Migratory Bird Sanctuary in the South Korea
Authors: Kim Hye Seon , Kim Haryun
Published: April 2021

This study investigated the environmental characteristics and macrobenthic community structures of an intertidal flat located on Yubu Island in the western coastal area of the Yellow Sea of South Korea. The study site has been well known for the migratory bird protection designated by UNESCO. We collected the samples of surface and depth core sediments and macrobenthos from the unused saltern and the area surrounding it in July 2017. Our results demonstrated that the means of mud, total carbon, and total nitrogen contents were 50%, 0.35% and 0.05%, respectively, and the origin of sediment composed of marine sources. The sedimentation rate ranged from 0.99 to 1.66 cm y−1, which was higher than other tidal flats in the Yellow Sea. The result of a topographic analysis indicated that the area in the unused saltern was 289.489 m2, and the length and width were 625 m and 500 m, respectively. A total of 13 species occurred with a mean density of 92 ind. m−2 and a mean biomass of 58.6 gWWt m−2. The Heteromastus filiformis (23.1%, 21 ind. m−2) was the most dominant species, followed by Perinereis linea (19.2%, 18 ind. m−2) and Tubuca arcuata (17.3%, 16 ind. m−2). Thus, this environmental information will help to establish policies to protect migratory birds in the future.
Effects of R&D Investments and Market Signals on International Acquisitions: Evidence from IPO Firms
Authors: Wu Cheng-Wei , Reuer Jeffrey J.
Published: April 2021

We investigate how intangible assets in the form of R&D influence firms’ hazards of engaging in international acquisitions. On the one hand, previous research has noted that the tacit and redeployable nature of R&D investments may prompt firms to expand their operations overseas and create value from international acquisitions. On the other hand, it is difficult for other firms to evaluate the quality and prospects of an acquirer’s intangible resources, thereby hampering its ability to finance and execute international M&A deals. In the context of international acquisitions undertaken by firms just completing their initial public offerings (IPOs), we argue and find that the IPO firm’s engagement in post-IPO international acquisition activity is generally negatively related to its R&D intensity. This effect contrasts previous arguments on the internalization advantages possessed by R&D-intensive firms. We also argue that firms able to convey their resources and prospects through such signals as previous international alliances and foreign sales can mitigate information problems presented by their intangibles, and thus carry out and benefit from cross-border acquisitions. We therefore identify an unexamined tradeoff that R&D investments present in the international M&A context and discuss how international signals can facilitate cross-border transactions subject to various market frictions.
Production of Hybrid Joints by Selective Laser Melting of Maraging Tool Steel 1.2709 on Conventionally Produced Parts of the Same Steel
Authors: Kučerová Ludmila , Zetková Ivana , Jeníček Štěpán , Burdová Karolína
Published: April 2021

Joining additively manufactured (AM) complex shaped parts to larger conventionally produced parts can lead to innovative product designs. Another alternative is direct deposition on a conventional semi-product. Therefore, similar joints of maraging tool steel 1.2709 were produced by AM deposition of powder of this steel on a bulk conventionally manufactured steel part. The resulting hybrid parts were solution annealed and precipitation hardened. Solution annealing at 820 °C for 20 min was followed by furnace cooling. Precipitation hardening was performed at 490 °C for 6 h. The mechanical properties of the samples were characterised using tensile testing and hardness measurement across the joint. Metallographic analysis was also carried out. The tensile properties of the AM and conventionally produced steel after equivalent heat treatments were also determined as the reference values. The mechanical properties of the hybrid parts are close to the properties of both steels. The hybrid parts in the as-built condition had a tensile strength of 1029 MPa and a total elongation of 14%. Solution annealing did not change these properties significantly, except for yield strength, which decreased by approximately 150 MPa. After precipitation annealing, the strength was higher, 2011 MPa, and total elongation dropped to 5%.
Investigation of Microcrack Propagation and Energy Evolution in Brittle Rocks Based on the Voronoi Model
Authors: Liu Guanlin , Chen Youliang , Du Xi , Xiao Peng , Liao Shaoming , Azzam Rafig
Published: April 2021

The cracking of rock mass under compression is the main factor causing structural failure. Therefore, it is very crucial to establish a rock damage evolution model to investigate the crack development process and reveal the failure and instability mechanism of rock under load. In this study, four different strength types of rock samples from hard to weak were selected, and the Voronoi method was used to perform and analyze uniaxial compression tests and the fracture process. The change characteristics of the number, angle, and length of cracks in the process of rock failure and instability were obtained. Three laws of crack development, damage evolution, and energy evolution were analyzed. The main conclusions are as follows. (1) The rock’s initial damage is mainly caused by tensile cracks, and the rapid growth of shear cracks after exceeding the damage threshold indicates that the rock is about to be a failure. The development of micro-cracks is mainly concentrated on the diagonal of the rock sample and gradually expands to the middle along the two ends of the diagonal. (2) The identification point of failure precursor information in Acoustic Emission (AE) can effectively provide a safety warning for the development of rock fracture. (3) The uniaxial compression damage constitutive equation of the rock sample with the crack length as the parameter is established, which can better reflect the damage evolution characteristics of the rock sample. (4) Tensile crack requires low energy consumption and energy dispersion is not concentrated. The damage is not apparent. Shear cracks are concentrated and consume a large amount of energy, resulting in strong damage and making it easy to form macro-cracks.
Reinforced 3D Composite Structures of γ-, α-Al2O3 with Carbon Nanotubes and Reduced GO Ribbons Printed from Boehmite Gels
Authors: Ramírez Cristina , Belmonte Manuel , Miranzo Pilar , Osendi Maria Isabel
Published: April 2021

The ability of boehmite to form printable inks has sparked interest in the manufacturing of 3D alumina (Al2O3) and composite structures by enabling direct ink writing methods while avoiding the use of printing additives. These materials may exhibit high porosity due to the printing and sintering procedures, depending on the intended application. The 3D-printed porous composite structures of γ-Al2O3 and α-Al2O3 containing 2 wt.% of carbon nanotubes or reduced graphene oxide ribbons were fabricated from boehmite gels, followed by different heat treatments. The reinforcing effect of these carbon nanostructures was evidenced by compression tests carried out on the different alumina structures. A maximum relative increase of 50% in compressive strength was achieved for the γ-Al2O3 composite structure reinforced with reduced graphene oxide ribbons, which was also accompanied by an increase in the specific surface area.
The Role of Carbon Nanotube Pretreatments in the Adsorption of Benzoic Acid
Authors: De Luca Pierantonio , Siciliano Carlo , Macario Anastasia , Nagy Jànos B.
Published: April 2021

Four different types of multi-walled carbon nanotubes (MWCNTs) were used and compared for the treatment of benzoic acid contaminated water. The types of nanotubes used were: (1) non-purified (CNTsUP), as made; (2) purified (CNTsP), not containing the catalyst; (3) oxidized (CNTsOX), characterized by the presence of groups such as, –COOH; (4) calcined (CNTs900), with elimination of interactions between nanotubes. In addition, activated carbon was also used to allow for later comparison. The adsorption tests were conducted on an aqueous solution of benzoic acid at concentration of 20 mg/L, as a model of carboxylated aromatic compounds. After the adsorption tests, the residual benzoic acid concentrations were measured by UV-visible spectrometry, while the carbon nanotubes were characterized by TG and DTA thermal analyses and electron microscopy (SEM). The results show that the type of nanotubes thermally treated at 900 °C has the best performances in terms of adsorption rate and amounts of collected acid, even if compared with the performance of activated carbons.