bluuebunny/crossref_metadata_2025 huggingface dataset:
Bacillus thuringiensis PM25 ameliorates oxidative damage of salinity stress in maize via regulating growth, leaf pigments, antioxidant defense system, and stress responsive gene expression
Authors: Ali Baber , Hafeez Aqsa , Ahmad Saliha , Javed Muhammad Ammar , Sumaira  , Afridi Muhammad Siddique , Dawoud Turki M. , Almaary Khalid S. , Muresan Crina Carmen , Marc Romina Alina , Alkhalifah Dalal Hussien M. , Selim Samy
Published: July 2022

Soil salinity is the major abiotic stress that disrupts nutrient uptake, hinders plant growth, and threatens agricultural production. Plant growth-promoting rhizobacteria (PGPR) are the most promising eco-friendly beneficial microorganisms that can be used to improve plant responses against biotic and abiotic stresses. In this study, a previously identified B. thuringiensis PM25 showed tolerance to salinity stress up to 3 M NaCl. The Halo-tolerant Bacillus thuringiensis PM25 demonstrated distinct salinity tolerance and enhance plant growth-promoting activities under salinity stress. Antibiotic-resistant Iturin C (ItuC) and bio-surfactant-producing (sfp and srfAA) genes that confer biotic and abiotic stresses were also amplified in B. thuringiensis PM25. Under salinity stress, the physiological and molecular processes were followed by the over-expression of stress-related genes (APX and SOD) in B. thuringiensis PM25. The results detected that B. thuringiensis PM25 inoculation substantially improved phenotypic traits, chlorophyll content, radical scavenging capability, and relative water content under salinity stress. Under salinity stress, the inoculation of B. thuringiensis PM25 significantly increased antioxidant enzyme levels in inoculated maize as compared to uninoculated plants. In addition, B. thuringiensis PM25-inoculation dramatically increased soluble sugars, proteins, total phenols, and flavonoids in maize as compared to uninoculated plants. The inoculation of B. thuringiensis PM25 significantly reduced oxidative burst in inoculated maize under salinity stress, compared to uninoculated plants. Furthermore, B. thuringiensis PM25-inoculated plants had higher levels of compatible solutes than uninoculated controls. The current results demonstrated that B. thuringiensis PM25 plays an important role in reducing salinity stress by influencing antioxidant defense systems and abiotic stress-related genes. These findings also suggest that multi-stress tolerant B. thuringiensis PM25 could enhance plant growth by mitigating salt stress, which might be used as an innovative tool for enhancing plant yield and productivity.
EbMYBP1, a R2R3-MYB transcription factor, promotes flavonoid biosynthesis in Erigeron breviscapus
Authors: Zhao Yan , Zhang Guanghui , Tang Qingyan , Song Wanling , Gao Qingqing , Xiang Guisheng , Li Xia , Liu Guanze , Fan Wei , Li Xiaoning , Yang Shengchao , Zhai Chenxi
Published: July 2022

Erigeron breviscapus, a traditional Chinese medicinal plant, is enriched in flavonoids that are beneficial to human health. While we know that R2R3-MYB transcription factors (TFs) are crucial to flavonoid pathway, the transcriptional regulation of flavonoid biosynthesis in E. breviscapus has not been fully elucidated. Here, EbMYBP1, a R2R3-MYB transcription factor, was uncovered as a regulator involved in the regulation of flavonoid accumulation. Transcriptome and metabolome analysis revealed that a large group of genes related to flavonoid biosynthesis were significantly changed, accompanied by significantly increased concentrations of the flavonoid in EbMYBP1-OE transgenic tobacco compared with the wild-type (WT). In vitro and in vivo investigations showed that EbMYBP1 participated in flavonoid biosynthesis, acting as a nucleus-localized transcriptional activator and activating the transcription of flavonoid-associated genes like FLS, F3H, CHS, and CHI by directly binding to their promoters. Collectively, these new findings are advancing our understanding of the transcriptional regulation that modulates the flavonoid biosynthesis.
Strategic outsourcing's role in driving economic value by examining mediating role of organizational capabilities and sustainable innovation
Authors: Shi Lei
Published: July 2022

The study's overarching purpose was to investigate the impact of strategic HR outsourcing on organizational sustainability. This study also attempted to evaluate the function of organizational capabilities and HR proficiency as a mediator in the relationship between strategic HR outsourcing and organizational sustainability. Data was collected from 400 HR professionals in China using a questionnaire technique. The Smart-PLS software and a structural equation modeling (SEM) technique were used to evaluate the data. Organizational sustainability was found to be insignificantly related to strategic HR outsourcing. Furthermore, organizational capabilities and HR proficiency were discovered to mediate the association between strategic HR outsourcing and organizational sustainability. By analyzing the impact of strategic HR outsourcing on organizational sustainability, this paper provided an important theoretical contribution. In terms of practical applications, this article would surely help HR professionals to maintain and develop policies to encourage employees to be engaged and perform well. Furthermore, this study might be effective in resolving difficulties linked to organizational sustainability. The small sample size of this study, which included solely HR professionals working in Chinese firms, was one of its limitations. In addition, future studies can incorporate other constructs to acquire a deeper knowledge of the factors that influence employee health.
Impact of brand hate on consumer well-being for technology products through the lens of stimulus organism response approach
Authors: Attiq Saman , Hamid Abu Bakar Abdul , Shah Hassan Jalil , Khokhar Munnawar Naz , Shahzad Amna
Published: July 2022

Consumer well-being is a micromarketing concept that emphasizes on contributions of marketing activities in social welfare. The major objective of the current study is to analyze the impact of self-incongruence on brand dissatisfaction, brand hate, and consumer well-being. This study has utilized the Self-incongruity Theory and the Stimulus-Organism-Response model to test the impact of self-incongruity on anti-consumption and consumer voice behaviors, and subsequent effects on consumer well-being. Data were collected from young consumers of technology products from major cities of Pakistan. A total of 592 consumers answered a paper-and-pencil questionnaire using purposive sampling technique. The data were analyzed by partial least square structural equation modeling. The findings of this study reveal that functional and symbolic incongruity predict brand hate and dissatisfaction, which is positively related with brand retaliation. Brand retaliation is negatively related with consumer well-being. This study offers implications for product designers, marketers, advertisers and other stakeholders to improve congruence between what young consumers of technology products expect and what brands are offering to mitigate negative attitudes and behaviors and increase consumer well-being.
Episodic memory training in elderly: A systematic review
Authors: Mendonça André Rocha , Loureiro Lucas Machado , Nórte Carlos Eduardo , Landeira-Fernandez Jesus
Published: July 2022

Introduction:

Episodic memory is a cognitive process that allows the recall of experiences, learning, and the pursuit of future goals. During the aging process, episodic memory declines negatively, impacting social and psychological aspects in the elderly. Such intervention strategies as cognitive training are non-pharmacological ways of reducing these losses.

Objective

We systematically reviewed studies of the cognitive training of episodic memory in healthy elderly individuals and elderly individuals with clinical conditions.

Method

We systematically searched the PubMed, PsycNET, Web of Science, and SciELO databases using the descriptors “Episodic Memory” AND “Training” AND “Elderly” OR “Aging” OR “Dementia” in English and translated into Portuguese.

Results

Of the 572 articles that were identified by the search, 23 were included in the final analysis. The main variables that comprised the intervention protocols (i.e., characteristics of the sample, type of control group, mode of episodic memory training, and duration of training) were investigated, as well as the outcome variables, efficacy, and risk of bias. The main results indicated effectiveness with regard to different forms of the cognitive training of episodic memory.

Conclusion

Episodic memory training among the elderly can mitigate the negative effects of cognitive decline in dementia and healthy subjects promoting impacts at social, psychological, and economic levels. Although it is a widely studied topic, further empirical studies on the utility of episodic memory training in healthy elderly individuals and elderly individuals with clinical conditions are still needed.
The relationship between distributed leadership and teacher innovativeness: Mediating roles of teacher autonomy and professional collaboration
Authors: Lin Qi
Published: July 2022

Principals’ distributed leadership plays a critical role in teacher innovativeness; however, research evidence regarding the relationship between them is limited. This study aims at examining the effect of distributed leadership on teacher innovativeness as well as the mediating roles of teacher autonomy and professional collaboration. Using the data of 132,376 teachers derived from the 2018 Teaching and Learning International Survey (TALIS), the study applied a structural equation model (SEM) for analysis. The results revealed that distributed leadership had positive direct effects on teacher innovativeness, teacher autonomy, and professional collaboration. Meanwhile, teacher autonomy and professional collaboration significantly mediated the effect of distributed leadership on teacher innovativeness, respectively. Practical implications are discussed, school leaders are expected to adopt distributed leadership style and establish a supportive school environment, and individual teachers are supposed to cultivate a culture of collectivism and make effective use of autonomy in their teaching innovation.
An artificial intelligence method for comprehensive evaluation of preschool education quality
Authors: Niu Peilin
Published: July 2022

The evolution in the quality of teaching for preschool education is worth studying. In this article, we solved the qualitative problems in the comprehensive quality evaluation by suggesting a method of quantitative combination and establishing a set of indicators suitable for the comprehensive quality evaluation of students in the kindergarten. According to the experience summed up by previous scholars, the weight of each index is obtained by an analytic hierarchy process. This study analyzed the defects and causes of fuzzy comprehensive evaluation and the neural network model in the construction of early childhood and preschool education's comprehensive quality evaluation model and propose a Feedforward Neural Network (FNN) model. FNN combined with neural network (NN) and fuzzy logic characteristics introduces fuzzy concepts and fuzzy inference rules into neural networks of neurons, the connection power, and network learning. It improves the learning ability of NN and fuzzy evaluation of the power of expression and effectively exerts the advantages of fuzzy logic and neural network to make up for their shortcomings. However, the convergence speed is very slow. To solve this problem, the similarity measure was used to improve the number of hidden layer nodes of the network. The effectiveness and feasibility of the FNN improved hidden layer nodes are verified by an example so as to realize the automation of comprehensive quality evaluation.
Development and validation of the care challenge scale in family caregivers of people with Alzheimer's disease
Authors: Sharif Nia Hamid , Sivarajan Froelicher Erika , Hosseini Lida , Ashghali Farahani Mansoureh , Hejazi Sima
Published: July 2022

Background

Alzheimer's disease (AD) is a progressive and debilitating disorder that strongly affects people with AD and their families. The changes in signs of the disease and its treatment lead to many challenges in people with AD that affect the performance and the ability of caregivers, their social life, and physical, emotional, and psychological aspects of caregivers' health. Therefore, this study was designed to develop and validate the Care Challenge Scale (CCS) for family caregivers of people with AD in the care context of Iran.

Method

This is a cross-sectional study, and the primary scale was based on 14 semi-structured interviews with family caregivers of Iranian people with AD. In the next phase, the psychometric features were assessed, including the face validity (qualitative and quantitative), content validity (qualitative and quantitative), item analysis, structural validity (exploratory and confirmatory factors), and construct validity (convergent and discriminant validity). Finally, the reliability was assessed using internal consistency (Cronbach's alpha, McDonald's omega coefficient, and the average inter-item correlation), stability (intraclass correlation coefficient), and absolute reliability.

Results

Totally, 435 Iranian family caregivers filled out online questionnaires, with a mean age of 50.26(±13.24) years. Based on the results of the qualitative phase, an item pool was generated with 389 items, and after deleting overlapping and unrelated items, the CCS with 14 items was created. The results of the quantitative phase showed that the CCS consists of two factors with 10 items each, which are named effective role-play challenge and lack of social–financial support, and they explained 42.23% of the total variance. Furthermore, the results of confirmatory factor analysis showed a good fitness of the scale structure model, and it had convergent and discriminant validity. The reliability indexes showed this scale has internal consistency and stability.

Conclusion

The most care challenge among Iranian family caregivers of people with AD is effective role-play challenges and lack of social–financial support. The scale as designed has good validity, internal consistency, and stability that can be used by therapists, nurses, and researchers for the assessment of the challenges of this population.
Salivary hormone concentrations and technical-tactical performance indicators in beach volleyball: Preliminary evidence
Authors: Costa Yago , Domingos-Gomes Jarbas , Lautenbach Franziska , Hayes Lawrence , Nakamura Fabio , Lima Jefferson , Castellano Lúcio , Batista Gilmário
Published: July 2022

The present study aimed to investigate (i) differences in salivary testosterone and cortisol concentrations before, during, and after simulated beach volleyball match, depending on match outcome (winning vs. losing); (ii) the relationship between technical-tactical performance indicators in beach volleyball and salivary hormonal concentrations (i.e., testosterone, cortisol). We hypothesized (i) salivary testosterone concentrations would be greater in winners and salivary cortisol would be lower; (ii) testosterone would associate with positive technical-tactical performance and cortisol would associate with negative technical-tactical performance. Sixteen athletes participated in the study and were grouped according to the result of a simulated game (winners: n = 8; losers: n = 8). Salivary hormone concentration of testosterone and cortisol were measured by enzyme-linked immunosorbent assay (pre-match, post first set, and post-match), and the coefficient of performance and efficiency were used as technical-tactical performance indicators. Regarding testosterone, there was a large effect size for match outcome after the first set (i.e., Winner vs. Losers) and a moderate effect size for the time in winners (pre-match vs. post-match). Regarding cortisol, there was a moderate effect size of time in losers only (pre-match vs. post-match). Moreover, cortisol pre-match was negatively correlated with the offensive performance (attack performance coefficient: r = −0.541; p = 0.030; attack efficiency: r = −0.568; p = 0.022). In conclusion, the effect of match outcome on testosterone and cortisol levels was moderate in winners and losers, respectively. Moreover, resting cortisol concentration appears to be related to a diminished attack technical-tactical performance. However, larger confirmatory studies are required to confirm these data to corroborate winning increases testosterone levels and/or reduces cortisol in a sporting setting.
Characterization of the Fecal Microbiome in Dogs Receiving Medical Management for Congenital Portosystemic Shunts
Authors: Squire Nathan , Lux Cassie , Tolbert Katie , Lidbury Jonathan , Sun Xiaocun , Suchodolski Jan S.
Published: July 2022

Background

The GI microbiome has not been characterized in dogs being medically managed for congenital portosystemic shunts (CPSS).

Objectives

To characterize the fecal microbiome in a population of dogs being medically managed for CPSS.

Animals

27 client-owned dogs.

Methods

Prospective cohort study enrollment of fecal samples was performed with follow-up data collected retrospectively. The overall fecal dysbiosis index (DI) and individual bacterial abundances were determined using real-time qPCR. Medical management, clinical findings, clinicopathologic, and outcome variables were collected, and logistic regression analyses were performed to evaluate associations between these variables and overall DI and bacterial abundances. Numerical variables were evaluated with general linear models for normality and equal variance using Shapiro-Wilk test and Levene's test, respectively.

Results

All dogs were administered a hepatic diet and lactulose, while antibiotics were used in 22 (81.5%) and acid suppressants in 7 (25.9%). Seventeen dogs (63.0%) had a DI >2. The median DI in this population was 3.02 (range 4.23–8.42), and the median DI in dogs receiving and not receiving antibiotics was 4.3 (range −4.23–8.42) and 1.52 (range −1.62–5.43), respectively. No significant association between any of the analyzed variables and the DI was identified. There was a significant association between the use of metronidazole and a larger abundance of E. coli (p = 0.024).

Conclusions and Clinical Importance

Dysbiosis appears to be common in dogs that are being medically managed for CPSS, though the clinical significance remains unclear.
Variations in the fecal microbiota and their functions of Thoroughbred, Mongolian, and Hybrid horses
Authors: Wen Xiaohui , Luo Shengjun , Lv Dianhong , Jia Chunling , Zhou Xiurong , Zhai Qi , Xi Li , Yang Caijuan
Published: July 2022

The horse gut is colonized by a rich and complex microbial community that has important roles in horse physiology, metabolism, nutrition, and immune functions. Fewer across-breed variations in horse gut microbial diversity have been illustrated. In this article, the gut microbiota of Thoroughbred, Mongolian, and Hybrid horses [first filial generation (F1) of Mongolian (maternal) and Thoroughbred (paternal)] were studied by second-generation high-throughput sequencing technology. Differences in gut microbiota composition and function between breeds were determined using diversity and functional prediction analysis. The alpha diversity analysis showed that Thoroughbred horses had a more abundant and diverse gut microbiota, while the diversity of gut microbiota in Hybrid horses was intermediate between Thoroughbred and Mongolian horses. Subsequent cluster analysis showed that Hybrid horses have a microbiota composition more similar to Mongolian horses. LEfSe analysis revealed that the bacterial biomarkers for Thoroughbred horses at the family level were Prevotellaceae, Rikenellaceae, Fibrobacteraceae, p_251_o5, Lactobacillaceae, and uncultured_bacterium_o_WCHB1_41; the bacterial biomarker for Mongolian horses was Planococcaceae; and the bacterial biomarkers for Hybrid horses were Moraxellaceae, Enterobacteriaceae, and Ruminococcaceae. The functional prediction results indicated that the metabolic pathways differ significantly between the breeds. Regarding metabolism, the Hybrid horses had the lowest proportion of the carbohydrate metabolic pathways, while the energy metabolic pathway had the highest proportion. The abundance ratios of the remaining eight metabolic pathways in Hybrid horses were between Thoroughbred and Mongolian horses. In conclusion, the results of this study showed an association between horse breeds and gut microbiota.
Anogenital scent-marking signals fertility in a captive female Alaotran gentle lemur
Authors: Fontani Sara , Kaburu Stefano S. K. , Marliani Giovanna , Accorsi Pier Attilio , Vaglio Stefano
Published: July 2022

The Lake Alaotra gentle lemur (Hapalemur alaotrensis) is one of the 25 most endangered primates in the world and shows low success rate in captive breeding programmes. It is therefore vital to further understand its reproductive biology. We studied a captive troop consisting of five individuals hosted at Jersey Zoo during breeding and non-breeding periods over 1 year. We collected behavioural data (n = 318 h) using all occurrence of some behaviours and ad libitum sampling methods, as well as faecal (n = 54) and anogenital scent (n = 35) samples of the breeding female. We measured sex hormone levels using enzyme immunoassay technique and investigated the volatile component of odour signals using solid-phase microextraction and gas chromatography-mass spectrometry. We observed sexual and aggressive behaviours occasionally during the breeding period. Our regression analysis showed that only period significantly predicted rates of female anogenital scent-marking, whereby the female performed anogenital scent-marking more frequently during the breeding rather than the non-breeding period. In contrast, female hormone levels did not significantly explain variation in rates of neither male nor female olfactory, sexual and affiliative behaviours, suggesting that individuals' behaviour alone is not an effective indicator of the ovulation window. The volatile chemical profile of anogenital odour secretions changed over the study, with four compounds distinguishing the fertile window during the breeding period. In conclusion, our findings suggest that anogenital scent-marking may signal the reproductive status of captive female gentle lemurs.
Case Report: Congenital tuberculosis in an aborted dromedary camel fetus
Authors: Narnaware Shirish Dadarao , Jyotsana Basanti , Ranjan Rakesh , Prakash Ved , Choudhary Shyam Sundar , Sahoo Artabandhu
Published: July 2022

Tuberculosis (TB) is a serious public health problem worldwide, especially in tropical developing countries. Nevertheless, reports on congenital TB in humans and animals are extremely rare. In this study, abortion was reported in an 8-year-old she-camel at the 9th month of gestation. The she-camel appeared healthy in clinical examination, had a good body condition score, normal appetite, and had no signs of respiratory disease and fever. The expelled placenta was dark red-colored, thickened, and edematous with multifocal to coalescing ecchymotic hemorrhages on the allantoic surface. The striking finding was multiple, white-yellow, solid nodular lesions in the fetal lung, the pleura, and the liver. On histopathology, typical granulomatous lesions were detected in the lung and the liver characterized by caseous necrosis surrounded by lymphocyte and macrophage infiltration and concentric layers of fibrosis. The Ziehl-Neelsen staining detected scarce acid-fast bacilli in lung and liver tissues. The DNA extracted from tubercular lesions from the lung and liver showed amplification of the IS6110 region of the Mycobacterium tuberculosis complex by PCR. The sequencing and phylogenetic analysis revealed a close association of these sequences with Mycobacterium tuberculosis. The she-camel was detected positive for a single intradermal tuberculin test performed 24 h after abortion. This is the first report on congenital TB caused by M. tuberculosis in a dromedary camel fetus with a possible vertical transmission.
Numerical Investigation on Cooling Performance of Rectangular Channels Filled with X-Shaped Truss Array Structures
Authors: Xi Lei , Gao Jianmin , Xu Liang , Zhao Zhen , Yang Zhengheng , Li Yunlong
Published: July 2022

In this study, different layout schemes for an X-shaped truss array channel are designed to explore the application of an X-shaped truss array structure in the mid-chord region of turbine blades. The flow and heat transfer performance of X-shaped truss array channels for three layout schemes are numerically investigated. The influence laws of the subchannel height ratio (h/H, 0.2 to 0.4) regarding the cooling performance of the channel with three subchannels are also analyzed. Then, the corresponding heat transfer and friction correlations are obtained. The results show that the layout scheme has significant effects on the flow performance, heat transfer performance and comprehensive thermal performance of X-shaped truss array channels. Among the three layout schemes of X-shaped truss array channels, the single channel has the best flow performance, while the channel with three subchannels has the best heat transfer performance and a comprehensive thermal performance. At different Reynolds numbers, the average Nusselt numbers and comprehensive thermal coefficients of the X-shaped truss array channel with three subchannels range from 38.94% to 63.49% and 27.74% to 46.49% higher than those of a single channel, respectively, and from 5.68% to 18.65% and 11.61% to 21.96% higher than those of the channel with two subchannels, respectively. For the channel with three subchannels, the subchannel height ratio has a great influence on the flow performance, but has a relatively small influence on the heat transfer performance and comprehensive thermal performance of the channel. With the increase in subchannel height ratio, the friction coefficient and average Nusselt number of the channel with three subchannels both show a trend of first increasing and then decreasing, while the comprehensive thermal coefficient shows a slow decreasing trend at higher Reynolds numbers. As a result of comprehensive consideration, the channel with three subchannels at a subchannel height ratio of 0.25 has a better overall cooling performance and is more suitable for cooling the mid-chord region of gas turbine blades. The results may provide a reference for the application of truss array structures in the internal cooling of advanced high-temperature turbine blades in the future.
Phish Responder: A Hybrid Machine Learning Approach to Detect Phishing and Spam Emails
Authors: Dewis Molly , Viana Thiago
Published: July 2022

Using technology to prevent cyber-attacks has allowed organisations to somewhat automate cyber security. Despite solutions to aid organisations, many are susceptible to phishing and spam emails which can make an unwanted impact if not mitigated. Traits that make organisations susceptible to phishing and spam emails include a lack of awareness around the identification of malicious emails, explicit trust, and the lack of basic security controls. For any organisation, phishing and spam emails can be received and the consequences of an attack could result in disruption. This research investigated the threat of phishing and spam and developed a detection solution to address this challenge. Deep learning and natural language processing are two techniques that have been employed in related research, which has illustrated improvements in the detection of phishing. Therefore, this research contributes by developing Phish Responder, a solution that uses a hybrid machine learning approach combining natural language processing to detect phishing and spam emails. To ensure its efficiency, Phish Responder was subjected to an experiment in which it has achieved an average accuracy of 99% with the LSTM model for text-based datasets. Furthermore, Phish Responder has presented an average accuracy of 94% with the MLP model for numerical-based datasets. Phish Responder was evaluated by comparing it with other solutions and through an independent t-test which demonstrated that the numerical-based technique is statistically significantly better than existing approaches.
Interval Fejér-Type Inequalities for Left and Right-λ-Preinvex Functions in Interval-Valued Settings
Authors: Saeed Tareq , Khan Muhammad Bilal , Treanțǎ Savin , Alsulami Hamed H. , Alhodaly Mohammed Sh.
Published: July 2022

For left and right λ-preinvex interval-valued functions (left and right λ-preinvex IVFs) in interval-valued Riemann operator settings, we create Hermite–Hadamard (H-H) type inequalities in the current study. Additionally, we create Hermite–Hadamard–Fejér (H-H-Fejér)-type inequalities for preinvex functions of the left and right interval-valued type under some mild conditions. Moreover, some exceptional new and classical cases are also obtained. Some useful examples are also presented to prove the validity of the results.
Multi-State Synchronization of Chaotic Systems with Distributed Fractional Order Derivatives and Its Application in Secure Communications
Authors: Kekha Javan Ali Akbar , Zare Assef , Alizadehsani Roohallah
Published: July 2022

This study investigates multiple synchronizations of distributed fractional-order chaotic systems. These systems consider unknown parameters, disturbance, and time delays. A robust adaptive control method is designed for multistage distributed fractional-order chaotic systems. In this paper, system parameters are changed step by step. Using Lyapunov’s function, while the synchronization error convergence to zero is guaranteed, adaptive rules are designed to estimate the parameters. Then, a secure communication scheme is proposed using the new chaotic masking method. Finally, the simulations are performed on a chaotic system of distributed Duffing fractional order. The results show the high efficiency of the proposed synchronization scheme using robust adaptive control, despite the parametric uncertainties, external disturbance, and variable and unknown time delays. Then, the simulations were performed on the sinusoidal signals of the message in the application of secure communications. The results showed the success of the proposed masking scheme with synchronization in coding and decoding information.
Treatment of Tooth Wear Using Direct or Indirect Restorations: A Systematic Review of Clinical Studies
Authors: Hardan Louis , Mancino Davide , Bourgi Rim , Cuevas-Suárez Carlos Enrique , Lukomska-Szymanska Monika , Zarow Maciej , Jakubowicz Natalia , Zamarripa-Calderón Juan Eliezer , Kafa Laura , Etienne Olivier , Reitzer François , Kharouf Naji , Haïkel Youssef
Published: July 2022

Tooth wear is considered a well-developed issue in daily clinical practice; however, there is no standard protocol for treatment. The aim of this manuscript was to systematically review the literature to evaluate the clinical outcomes of direct or indirect restorations for treating tooth wear. A literature search was conducted through the PubMed MedLine, Scopus, ISI Web of Science, Scielo, and EMBASE databases up to 29 April 2022. Clinical studies evaluating the clinical performance of direct or indirect restorations for treating tooth wear for a minimum follow-up of 6 months were included in the review. A total of 2776 records were obtained from the search databases. After full-text reading, 16 studies were included in the qualitative analysis. Considering the high heterogenicity of the studies included, a meta-analysis could not be performed. All studies included the rehabilitation of anterior and posterior teeth with extensive wear, using both indirect and direct restorations for a maximum follow-up of 10 years. Restoration materials included ceramo-metal crowns, full gold crowns, lithium disilicate ceramic, zirconia, polymer infiltrated ceramic networks, and resin composites. Most of the reports assessed the survival rate of the restorations and the clinical features using the United States Public Health Service (USPHS) Evaluation System criteria. Contradictory discoveries were perceived concerning the type of restoration with better clinical performance. Considering the current literature available, there is no evidence in the superiority of any restoration technique to ensure the highest clinical performance for treating tooth wear.
Long-Term Exposure to Ozone and Fine Particulate Matter and Risk of Premature Coronary Artery Disease: Results from Genetics of Atherosclerotic Disease Mexican Study
Authors: Posadas-Sánchez Rosalinda , Vargas-Alarcón Gilberto , Cardenas Andres , Texcalac-Sangrador José Luis , Osorio-Yáñez Citlalli , Sanchez-Guerra Marco
Published: July 2022

(1) Background: Epidemiological studies have identified associations between fine particulate matter (PM2.5) and ozone exposure with cardiovascular disease; however, studies linking ambient air pollution and premature coronary artery disease (pCAD) in Latin America are non-existing. (2) Methods: Our study was a case–control analysis nested in the Genetics of Atherosclerotic Disease (GEA) Mexican study. We included 1615 participants (869 controls and 746 patients with pCAD), recruited at the Instituto Nacional de Cardiología Ignacio Chávez from June 2008 to January 2013. We defined pCAD as history of myocardial infarction, angioplasty, revascularization surgery or coronary stenosis > 50% diagnosed before age 55 in men and age 65 in women. Controls were healthy individuals without personal or family history of pCAD and with coronary artery calcification equal to zero. Hourly measurements of ozone and PM2.5 from the Atmospheric Monitoring System in Mexico City (SIMAT in Spanish; Sistema de Monitero Atmosférico de la Ciudad de México) were used to calculate annual exposure to ozone and PM2.5 in the study participants. (3) Results: Each ppb increase in ozone at 1-year, 2-year, 3-year and 5-year averages was significantly associated with increased odds (OR = 1.10; 95% CI: 1.03–1.18; OR = 1.17; 95% CI: 1.05–1.30; OR = 1.18; 95% CI: 1.05–1.33, and OR = 1.13; 95% CI: 1.04–1.23, respectively) of pCAD. We observed higher risk of pCAD for each 5 µg/m3 increase only for the 5-year average of PM2.5 exposure (OR = 2.75; 95% CI: 1.47–5.16), compared to controls. (4) Conclusions: Ozone exposure at different time points and PM2.5 exposure at 5 years were associated with increased odds of pCAD. Our results highlight the importance of reducing long-term exposure to ambient air pollution levels to reduce the burden of cardiovascular disease in Mexico City and other metropolitan areas.
Advances on Bacterial and Fungal Biofilms for the Production of Added-Value Compounds
Authors: Carvalho Fábio M. , Azevedo Ana , Ferreira Marta M. , Mergulhão Filipe J. M. , Gomes Luciana C.
Published: July 2022

In recent years, abundant research has been performed on biofilms for the production of compounds with biotechnological and industrial relevance. The use of biofilm platforms has been seen as a compelling approach to producing fine and bulk chemicals such as organic acids, alcohols, and solvents. However, the production of recombinant proteins using this system is still scarce. Biofilm reactors are known to have higher biomass density, operational stability, and potential for long-term operation than suspended cell reactors. In addition, there is an increasing demand to harness industrial and agricultural wastes and biorefinery residues to improve process sustainability and reduce production costs. The synthesis of recombinant proteins and other high-value compounds is mainly achieved using suspended cultures of bacteria, yeasts, and fungi. This review discusses the use of biofilm reactors for the production of recombinant proteins and other added-value compounds using bacteria and fungi.
A 500 kHz to 150 MHz Multi-Output Clock Generator Using Analog PLL and Open-Loop Fractional Divider with 0.13 μm CMOS
Authors: Jin Junting , Jin Yuhua , Gan Yebing
Published: July 2022

Clocks are widely used in multimedia and electronic devices, and they usually have different frequency demands. This paper presents the design of a multi-output clock generator using an analog integer-N phase-locked loop (PLL) and open-loop fractional dividers. The PLL based on a three-stage ring voltage-controlled oscillator (VCO) is used to transform the lower frequency reference into a high-frequency intermediate clock (600 MHz–900 MHz). Then, relying on the open-loop fractional divider, a wide frequency range of 500 kHz to 150 MHz can be generated. Due to the open-loop control characteristic, the clock generator has instantaneous frequency switching capability. In addition, phase-adjusting circuits added to the divider greatly improved the jitter performance of the output clock; its RMS jitter is 5.2 ps. This work was conducted with 0.13 μm CMOS technology. The open-loop divider occupies an area of 0.032 mm2 and consumes 7.7 mW from a 1.2 V supply.
Epigenomic Approaches for the Diagnosis of Rare Diseases
Authors: Martinez-Delgado Beatriz , Barrero Maria J.
Published: July 2022

Rare diseases affect more than 300 million people worldwide. Diagnosing rare diseases is a major challenge as they have different causes and etiologies. Careful assessment of clinical symptoms often leads to the testing of the most common genetic alterations that could explain the disease. Patients with negative results for these tests frequently undergo whole exome or genome sequencing, leading to the identification of the molecular cause of the disease in 50% of patients at best. Therefore, a significant proportion of patients remain undiagnosed after sequencing their genome. Recently, approaches based on functional aspects of the genome, including transcriptomics and epigenomics, are beginning to emerge. Here, we will review these approaches, including studies that have successfully provided diagnoses for complex undiagnosed cases.
Fractional Integral of the Confluent Hypergeometric Function Related to Fuzzy Differential Subordination Theory
Authors: Acu Mugur , Oros Gheorghe , Rus Ancuța Maria
Published: July 2022

The fuzzy differential subordination concept was introduced in 2011, generalizing the concept of differential subordination following a recent trend of adapting fuzzy sets theory to other already-established theories. A prolific tool in obtaining new results related to operators is the fractional integral applied to different functions. The fractional integral of the confluent hypergeometric function was previously investigated using means of the classical theory of subordination. In this paper, we give new applications of this function using the theory of fuzzy differential subordination. Fuzzy differential subordinations are established and their best dominants are also provided. Corollaries are written using particular functions, in which the conditions for the univalence of the fractional integral of the confluent hypergeometric function are given. An example is constructed as a specific application of the results obtained in this paper.
Onion Plant Size Measurements as Predictors for Onion Bulb Size
Authors: Nourbakhsh Seyed Shahabeddin , Cramer Christopher S.
Published: July 2022

Onion is a biennial plant that produces a bulb. The larger the onion bulb, the more valuable it becomes. Therefore, it is important to study bulb weight and plant size components affecting it. For this study, four New Mexico State Univ. breeding lines and two commercial cultivars, ‘Rumba’ and ‘Stockton Early Yellow’, were selected. These breeding lines and cultivars were evaluated for plant height, leaf number and sheath diameter, at five different dates two weeks apart throughout the growing season, and for bulb weight upon harvest. The experiment was designed as a randomized complete block design with three blocks each containing four replications. Plant size components were all positively correlated with each other and with bulb weight with correlation coefficients above 0.50. NMSU breeding lines also exhibited greater average bulb weights than commercial cultivars. Sheath diameter proved to be the best predictor of bulb size, showing strong positive correlations with bulb weight at around 12 weeks after transplanting, and positive correlations are observed as early as 6–8 weeks after transplanting.
Cervical Cancer Outcome and Tumor-Associated Macrophages: Research Evidence
Authors: Horta Bruno , Pereira Tomé , Medeiros Rui , Cerqueira Fátima
Published: July 2022

Inflammation is a key factor in cancer promotion. Tumor-associated macrophages (TAMs), as part of the tumor microenvironment, are often associated with the progression of tumors and a worse prognosis in many cancers, namely on cervical cancer. This work exhaustively summarizes the conclusions of the different studies published concerning TAMs function in cervical cancer, from in vitro studies using cancer cell lines to the clinical perspective (histological samples-based studies). Most studies have led to the conclusion that TAMs increased density is directly related to increased severity of a malignant cervical lesion. Additionally, TAMs are normally polarized into an M2 phenotype, benefiting and promoting tumor progression, resulting in a worse disease outcome. The tumor microenvironment is also a highly critical contributor that not only influences tumor natural history but also modulates the specific immune response.
The Relationship of Maternal Gestational Mass Spectrometry-Derived Metabolites with Offspring Congenital Heart Disease: Results from Multivariable and Mendelian Randomization Analyses
Authors: Taylor Kurt , McBride Nancy , Zhao Jian , Oddie Sam , Azad Rafaq , Wright John , Andreassen Ole A. , Stewart Isobel D. , Langenberg Claudia , Magnus Maria Christine , Borges Maria Carolina , Caputo Massimo , Lawlor Deborah A.
Published: July 2022

Background: It is plausible that maternal pregnancy metabolism influences the risk of offspring congenital heart disease (CHD). We sought to explore this through a systematic approach using different methods and data. Methods: We undertook multivariable logistic regression of the odds of CHD for 923 mass spectrometry (MS)-derived metabolites in a sub-sample of a UK birth cohort (Born in Bradford (BiB); N = 2605, 46 CHD cases). We considered metabolites reaching a p-value threshold <0.05 to be suggestively associated with CHD. We sought validation of our findings, by repeating the multivariable regression analysis within the BiB cohort for any suggestively associated metabolite that was measured by nuclear magnetic resonance (NMR) or clinical chemistry (N = 7296, 87 CHD cases), and by using genetic risk scores (GRS: weighted genetic risk scores of single nucleotide polymorphisms (SNPs) that were associated with any suggestive metabolite) in Mendelian randomization (MR) analyses. The MR analyses were performed in BiB and two additional European birth cohorts (N = 38,662, 319 CHD cases). Results: In the main multivariable analyses, we identified 44 metabolites suggestively associated with CHD, including those from the following super pathways: amino acids, lipids, co-factors and vitamins, xenobiotics, nucleotides, energy, and several unknown molecules. Of these 44, isoleucine and leucine were available in the larger BiB cohort (NMR), and for these the results were validated. The MR analyses were possible for 27/44 metabolites and for 11 there was consistency with the multivariable regression results. Conclusions: In summary, we have used complimentary data sources and statistical techniques to construct layers of evidence. We found that pregnancy amino acid metabolism, androgenic steroid lipids, and levels of succinylcarnitine could be important contributing factors for CHD.
Electroporation Parameters for Human Cardiomyocyte Ablation In Vitro
Authors: Baena-Montes Jara M. , O’Halloran Tony , Clarke Cormac , Donaghey Kevin , Dunne Eoghan , O’Halloran Martin , Quinlan Leo R.
Published: July 2022

Cardiac ablation with irreversible electroporation (IRE) is quickly being established as a modality of choice for atrial fibrillation treatment. While it has not yet been optimised, IRE has the potential to significantly limit collateral damage and improve cell-specific targeting associated with other energy sources. However, more tissue and cell-specific evidence is required to demonstrate the selective threshold parameters for human cells. The aim here is to determine the optimal ablation threshold parameters related to lesion size for human cardiomyocytes in 2D culture. Conventional biphasic pulses of different field strengths and on-times were delivered in a monolayer culture system of human AC16 cardiomyocytes. The dynamics of cell death and lesion dimensions were examined at different time points. Human cardiomyocytes are susceptible to significant electroporation and cell death at a field strength of 750 V/cm or higher with 100 μs pulses. Increasing the IRE on-time from 3 ms to 60 ms reduces the effective field threshold to 250 V/cm. Using very short pulses of 2 μs and 5 μs also causes significant cell death, but only at fields higher than 1000 V/cm. A longer on-time results in more cell death and induced greater lesion area in 2D models. In addition, different forms of cell death are predicted based on the evolution of cell death over time. This study presents important findings on the ability of different IRE parameters to induce human cardiomyocyte cell death. Lesion size can be tuned by appropriate choice of IRE parameters and cardiomyocytes display an upregulation of delayed cell death 24 h after electroporation, which is an important consideration for clinical practice.
Identifying the Resource Needs of Young People with Differences of Sex Development
Authors: Tonkin-Hill Gina , Hanna Chloe , Bonelli Roberto , Mortimer Rowena , O’Connell Michele A. , Grover Sonia R.
Published: July 2022

Adolescents with differences of sex development (DSD) often have complex medical, surgical, and psychological care needs and require age-appropriate resources. This cross-sectional study describes the past and current experiences of adolescents and young adults with DSD and their need for information and support. Participants aged 14–30 years with DSD diagnoses were identified, either from departmental records at the Royal Children’s Hospital (RCH), Melbourne, Australia, or from the private practice of a gynecologist linked to RCH. Anonymized data were collected from a specifically designed online survey. Of the 314 successfully traced patients, 91 (28.9%) completed the survey. Amongst respondents, older age was strongly correlated with higher levels of distress at the time of disclosure (b = 0.67, p < 0.001). People who reported greater understanding of their condition (b = −0.45, p = 0.010) and higher levels of support (b = −0.40, p = 0.003) identified lower levels of current distress. Respondents preferred to receive information from a specialist doctor, GP, or websites and reported information needs being highest during adolescence. Only one in four respondents recalled ever being offered psychological support. A number of perceived barriers to accessing support were identified. Our findings indicate that young people’s information and support needs may be best met by improving online resources, as well as increasing introductions to knowledgeable and appropriate primary care physicians, psychological services, and peer support groups. Further work to promote and increase engagement with psychological and peer support for those with DSD will be important.