bluuebunny/crossref_metadata_2025 huggingface dataset:
Short-term outcome for high-risk patients after esophagectomy
Authors: Plat Victor D , Stam Wessel T , Bootsma Boukje T , Straatman Jennifer , Klausch Thomas , Heineman David J , van der Peet Donald L , Daams Freek ,   Dutch Upper GI Cancer Audit Group
Published: June 2022

Transthoracic esophagectomy (TTE) for esophageal cancer facilitates mediastinal dissection; however, it has a significant impact on cardiopulmonary status. High-risk patients may therefore be better candidates for transhiatal esophagectomy (THE) in order to prevent serious complications. This study addressed short-term outcome following TTE and THE in patients that are considered to have a higher risk of surgery-related morbidity. This population-based study included patients who underwent a curative esophagectomy between 2011 and 2018, registered in the Dutch Upper GI Cancer Audit. The Charlson comorbidity index was used to assign patients to a low-risk (score ≤ 1) and high-risk group (score ≥ 2). Propensity score matching was applied to produce comparable groups between high-risk patients receiving TTE and THE. Primary endpoint was mortality (in-hospital/30-day mortality), secondary endpoints included morbidity and oncological outcomes. Additionally, a matched subgroup analysis was performed, including only cervical reconstructions. Of 5,438 patients, 945 and 431 high-risk patients underwent TTE and THE, respectively. After propensity score matching, mortality (6.3 vs 3.3%, P = 0.050), overall morbidity, Clavien-Dindo ≥ 3 complications, pulmonary complications, cardiac complications and re-interventions were significantly more observed after TTE compared to THE. A significantly higher mortality after TTE with a cervical reconstruction was found compared to THE (7.0 vs. 2.2%, P = 0.020). Patients with a high Charlson comorbidity index predispose for a complicated postoperative course after esophagectomy, this was more outspoken after TTE compared to THE. In daily practice, these outcomes should be balanced with the lower lymph node yield, but comparable positive node count and radicality after THE.
Histopathological validation of semi-automated myocardial scar quantification techniques for dark-blood late gadolinium enhancement magnetic resonance imaging
Authors: Nies Hedwig M J M , Gommers Suzanne , Bijvoet Geertruida P , Heckman Luuk I B , Prinzen Frits W , Vogel Gaston , Van De Heyning Caroline M , Chiribiri Amedeo , Wildberger Joachim E , Mihl Casper , Holtackers Robert J
Published: June 2022

Aims

To evaluate the performance of various semi-automated techniques for quantification of myocardial infarct size on both conventional bright-blood and novel dark-blood late gadolinium enhancement (LGE) images using histopathology as reference standard.

Methods and results

In 13 Yorkshire pigs, reperfused myocardial infarction was experimentally induced. At 7 weeks post-infarction, both bright-blood and dark-blood LGE imaging were performed on a 1.5 T magnetic resonance scanner. Following magnetic resonance imaging (MRI), the animals were sacrificed, and histopathology was obtained. The percentage of infarcted myocardium was assessed per slice using various semi-automated scar quantification techniques, including the signal threshold vs. reference mean (STRM, using 3 to 8 SDs as threshold) and full-width at half-maximum (FWHM) methods, as well as manual contouring, for both LGE methods. Infarct size obtained by histopathology was used as reference. In total, 24 paired LGE MRI slices and histopathology samples were available for analysis. For both bright-blood and dark-blood LGE, the STRM method with a threshold of 5 SDs led to the best agreement to histopathology without significant bias (−0.23%, 95% CI [−2.99, 2.52%], P = 0.862 and −0.20%, 95% CI [−2.12, 1.72%], P = 0.831, respectively). Manual contouring significantly underestimated infarct size on bright-blood LGE (−1.57%, 95% CI [−2.96, −0.18%], P = 0.029), while manual contouring on dark-blood LGE outperformed semi-automated quantification and demonstrated the most accurate quantification in this study (−0.03%, 95% CI [−0.22, 0.16%], P = 0.760).

Conclusion

The signal threshold vs. reference mean method with a threshold of 5 SDs demonstrated the most accurate semi-automated quantification of infarcted myocardium, without significant bias compared to histopathology, for both conventional bright-blood and novel dark-blood LGE.
Thymomectomy versus complete thymectomy in early-stage non-myasthenic thymomas: a multicentric propensity score-matched study
Authors: Voulaz Emanuele , Perroni Gianluca , Russo Anna , Patirelis Alexandro , Mangiameli Giuseppe , Alloisio Marco , Ambrogi Vincenzo
Published: June 2022

OBJECTIVES

Thymomectomy is gaining consensus over complete thymectomy in early-stage thymoma without myasthenia gravis. This is due both to the difficulty of establishing prospective and randomized controlled studies and to the lack of well-defined selection criteria. This bicentric, retrospective propensity score-matched study aims at comparing oncological outcomes, measured in terms of overall survival and thymoma-related survival, in patients undergoing either thymomectomy or complete thymectomy.

METHODS

We retrospectively analysed medical records of patients with clinical early-stage (I and II) thymoma undergoing thymomectomy or complete thymectomy. Exclusion criteria were the presence of myasthenia gravis, clinical advanced tumours and thymic carcinoma. A propensity score-matching analysis was applied to reduce potential preoperative selection biases such as comorbidity (Charlson score), tumour maximal diameter and surgical approach (open versus minimal). All variables were dichotomized.

RESULTS

A total of 255 patients were enrolled from 2 different Hospitals, 126 underwent complete thymectomy and 129 a thymomectomy. Disease-free and thymoma-related survivals showed a 5-year rate of 87.7% and 96.0% and a 10-year rate of 82.2% and 91.9%, respectively. Propensity score-matching analysis selected a total of 176 patients equally divided between the 2 groups. No difference was found for both disease-free (P = 0.11) and thymoma-related (P = 0.37) survival in the 2 groups of resection. Multivariable Cox regression analysis showed that histology (P < 0.001), residual disease (P < 0.001) and adjuvant chemotherapy (P < 0.001) were the only predictors of shorter disease-free survival. Whereas there was no evidence to confirm that disease-free and thymoma-related survivals were influenced by resection extent.

CONCLUSIONS

Thymomectomy is an adequate surgical resection for non-myasthenic thymoma, achieving disease-free and thymoma-related survivals comparable to those after complete thymectomy.
Omicron Breakthrough Infection After Heterologous Prime-Boost Vaccination Induces a Vigorous Antibody Response
Authors: Varese Augusto , Mazzitelli Bianca , Erra Díaz Fernando , Kjolhede María Victoria , Ojeda Diego , Vellicce Alejandra , Arto Penélope , Cicero Carla , Pascowsky María , Figueras Laura , Broese Bárbara , Dávila Rosa , Zarlenga Rocío , Rivelli Federico , Verruno Camila , Silenzi Valeria , Beltrán Ivana , Gamarnik Andrea , Ceballos Ana , Zala Carlos , Badolati Adelina , Geffner Jorge
Published: June 2022

Infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant is usually asymptomatic or mild and appears to be poorly immunogenic at least in unvaccinated individuals. Here, we found that health care workers vaccinated with 2 doses of Sputnik V and a booster dose of ChAdOx1 mount a vigorous neutralizing-antibody response after Omicron breakthrough infection.
Traditional remedies and other characteristics among human snakebite survivors in Baringo county, Kenya, 2010–2020: a case series
Authors: Kung'u Peris N , Chweya Reagan N , Gachohi John M
Published: June 2022

Background

Seeking traditional remedies following snakebites leads to avoidable deaths in rural settings in developing countries.

Methods

In this case series study, we identified and recruited 169 snakebite survivors in Baringo county, a hard-to-reach region in northwestern Kenya, who experienced snakebites from 2010 to 2020 using a snowballing technique. We explored associations between traditional and hospital care in managing snakebites and other characteristics. χ2 tests assessed these categorical differences.

Results

Fifty-four (33%) of the survivors used traditional remedies to manage snakebites. The majority (56%) were men and aged >18 y (72%); 59% had low education levels and income. They sourced water from rivers or lakes (93%) and used charcoal as an energy source (74%). These survivors (>67%) resided in households practicing free-range and stall-feeding animal husbandry systems and in houses with thatch roofing or an earthen floor structure. Also, >62% reported muscle tremors, fever and chills, while 80% visited health facilities for further treatment.

Conclusion

Community sensitization covering the risks of non-effective remedies and escalation of training to traditional healers could improve the speed of referrals in hard-to-reach snakebite hotspots. Medical anthropology studies could explore the enablers of continued use of traditional remedies in snakebite management in rural communities.
Observational Study of Receptor Binding Domain Spike Antibody Responses to 3 SARS-CoV-2 Vaccinations in Noninfected Subjects: Parallel Neutralizing Antibody and Cardiac Troponin I and T Observations
Authors: Apple Fred S , Bothwell Bryan , Koti Jaya , Bauer Westley , Dwyer Robert , Chen Haode , Wu Heng , Li Pu , Lindgren Britanny , Gottlieb Sydney , Okeson Brynn , Schulz Karen
Published: June 2022

Background

Our goals were to demonstrate receptor binding domain spike 1 (RBD S1) protein antibody (Ab) kinetic responses to multiple vaccines over approximately 180 days, neutralizing Ab effectiveness, and high-sensitivity cardiac troponin I (hs-cTnI) and T (hs-cTnT) responses in postvaccinated, non-SARS-CoV-2–infected subjects.

Methods

Blood specimens were collected pre- and postvaccinations from seronegative subjects. RDB S1 Abs were measured by the novel Qorvo Biotechnologies Omnia platform. Neutralizing Abs and hs-cTnI and hs-cTnT were measured on the ET Healthcare Pylon 3D.

Results

Two-dose vaccines (Pfizer, Moderna) had peak RBD S1 Ab concentrations about 45 to 55 days after both doses and showed declines over the next 50 to 70 days. The Janssen vaccine showed lower RBD S1 Ab peak concentrations, continued to increase over time, and plateaued after 60 days. There was strong neutralizing Ab response post vaccinations, with only 3 specimens, shortly before and shortly after vaccination, not showing a response. Specimens showed no hs-cTnI (all < 3 ng/L) and hs-cTnT (all < 6 ng/L) increases or changes over time.

Conclusions

We demonstrate in seronegative SARS-CoV-2 subjects that Pfizer and Moderna vaccinations provide strong, neutralizing RBD S1 Ab effectiveness, based on 2 different assays after 2 doses, with the Janssen single-dose vaccine showing a lower RBD S1 Ab response over 4 to 6 months. No myocardial injury was associated with the Pfizer postvaccination. The Qorvo Biotechnologies RBD S1 Ab assay measured on the Omnia platform has potential as a point-of-care platform.
Outcomes of Canceled Tomosynthesis-Guided Biopsy of Architectural Distortion Due to Nonvisualization
Authors: Myers Kelly S , Oluyemi Eniola T , Mullen Lisa A , Panigrahi Babita , Di Carlo Philip A , Nguyen Derek L , Ambinder Emily B
Published: June 2022

Objective

Architectural distortion without a sonographic correlate is an indication for digital breast tomosynthesis–guided vacuum-assisted biopsy (DBT-VAB). However, when the finding is not visualized on the day of biopsy, the procedure is canceled. This study reports the outcomes of canceled DBT-VAB of architectural distortion due to nonvisualization.

Methods

In this IRB-approved retrospective study, chart review was performed to identify DBT-VABs of architectural distortion at our institution between June 1, 2017, and November 1, 2020, that were canceled because of nonvisualization at the time of biopsy. Cases without follow-up imaging were excluded. Statistical analysis, including the frequency of cases yielding malignancy by the end of the study period, was performed.

Results

In total, 7.2% (39/544) of architectural distortions recommended for biopsy during the study period were canceled because of nonvisualization, 30 of which had follow-up imaging and were included in the study. Mean patient age was 56 years (standard deviation [SD], 9.6 years) and mean follow-up time was 26.7 months (SD, 11.2 months; range, 8.4–50.9 months). During the follow-up period, 16.7% (5/30) underwent repeat biopsy attempt, with one malignant result (1/30, 3.3%; SD, 18%; 95% confidence interval: 0.6%–16.7%). In total, 86.7% (26/30) of cases were declared benign during the follow-up period and 10% (3/30) remained stable with a BI-RADS 3 assessment category.

Conclusion

During available follow-up, there was a low likelihood that distortions not visualized at the time of DBT-VAB represented malignancy (3.3%, 1/30). While this low malignancy rate is reassuring, imaging follow-up is warranted.
Convergent loss of anthocyanin pigments is controlled by the same MYB gene in cereals
Authors: Li Yan , Fang Xiaojian , Lin Zhongwei
Published: June 2022

Loss of anthocyanin pigments is a common transition during cereal domestication, diversification, and improvement. However, the genetic basis for this convergent transition in cereal remains largely unknown. Here, we identified a chromosomal syntenic block across different species that contained R2R3-MYB genes (c1/pl1) responsible for the convergent decoloring of anthocyanins in cereals. Quantitative trait locus (QTL) mapping identified a major QTL for aerial root color corresponding to pl1 and a major QTL for spikelet color corresponding to c1 on maize chromosomes 6 and 9, respectively. One insertion in the regulatory region that led to transcriptional down-regulation was present in maize pl1, and several insertions in the coding region resulting in loss of function occurred in maize c1. A transposable element insertion in the third exon of c1, leading to three new non-functional transcripts, was responsible for decoloring in foxtail millet. The c1/pl1 genes enhanced the transcription of the core enzyme-encoding genes, including pr1, fht1, a1, a2, bz1, and aat1 in the anthocyanin pathway, while they repressed the expression of fnsii1 in flavones, sm2 in maysin, and bx3, bx4, bx5, and bx10 in DIMBOA. Our results indicated that the convergent decoloring of these plants shared the same genetic basis across different cereal species.
Rational domestication of a plant-based recombinant expression system expands its biosynthetic range
Authors: Jackson Mark A , Chan Lai Yue , Harding Maxim D , Craik David J , Gilding Edward K
Published: June 2022

Plant molecular farming aims to provide a green, flexible, and rapid alternative to conventional recombinant expression systems, capable of producing complex biologics such as enzymes, vaccines, and antibodies. Historically, the recombinant expression of therapeutic peptides in plants has proven difficult, largely due to their small size and instability. However, some plant species harbour the capacity for peptide backbone cyclization, a feature inherent in stable therapeutic peptides. One obstacle to realizing the potential of plant-based therapeutic peptide production is the proteolysis of the precursor before it is matured into its final stabilized form. Here we demonstrate the rational domestication of Nicotiana benthamiana within two generations to endow this plant molecular farming host with an expanded repertoire of peptide sequence space. The in planta production of molecules including an insecticidal peptide, a prostate cancer therapeutic lead, and an orally active analgesic is demonstrated.
The Ownership and Sale of Manuscripts of John Gower’s Confessio Amantis in the Eighteenth and Nineteenth Centuries
Authors: Edwards A S G
Published: June 2022

This article examines the evidence for the circulation and ownership of manuscripts of John Gower’s Middle English poem, Confessio Amantis, in the eighteenth and nineteenth centuries. The records of booksellers’ and auction catalogues indicate frequent sales of copies coupled with a generally limited commercial appeal. These records also suggest that some copies were in circulation in this period that cannot be identified with those that now survive. It has also proved possible to add information from sale records about the history of the ownership of individual copies of Gower’s poem.
The late-time light curves of Type Ia supernovae: confronting models with observations
Authors: Tiwari Vishal , Graur Or , Fisher Robert , Seitenzahl Ivo , Leung Shing-Chi , Nomoto Ken’ichi , Perets Hagai Binyamin , Shen Ken
Published: June 2022

Type Ia supernovae (SNe Ia) play a crucial role as standardizable candles in measurements of the Hubble constant and dark energy. Increasing evidence points towards multiple possible explosion channels as the origin of normal SNe Ia, with possible systematic effects on the determination of cosmological parameters. We present, for the first time, a comprehensive comparison of publicly available SN Ia model nucleosynthetic data with observations of late-time light curve observations of SN Ia events. These models span a wide range of white dwarf (WD) progenitor masses, metallicities, explosion channels, and numerical methodologies. We focus on the influence of 57Ni and its isobaric decay product 57Co in powering the late-time (t > 1000 d) light curves of SNe Ia. 57Ni and 57Co are neutron-rich relative to the more abundant radioisotope 56Ni, and are consequently a sensitive probe of neutronization at the higher densities of near-Chandrashekhar (near-MCh) progenitor WDs. We demonstrate that observations of one SN Ia event, SN 2015F is only consistent with a sub-Chandrasekhar (sub-MCh) WD progenitor. Observations of four other events (SN 2011fe, SN 2012cg, SN 2014J, and SN2013aa) are consistent with both near-MCh and sub-MCh progenitors. Continued observations of late-time light curves of nearby SNe Ia will provide crucial information on the nature of the SN Ia progenitors.
The local universe in the era of large surveys - II. multi-wavelength characterization of activity in nearby S0 galaxies
Authors: Jiménez-Palau C , Solanes J M , Perea J D , del Olmo A , Tous J L
Published: June 2022

This is the second paper in a series using data from tens of thousands S0 galaxies of the local Universe (z ≲ 0.1) retrieved from the NASA-Sloan Atlas. It builds on the outcomes of the previous work, which introduced a new classification scheme for these objects based on the principal component analysis (PCA) of their optical spectrum and its projections on to the first two eigenvectors or principal components (the PC1–PC2 diagram). We provide a comprehensive characterization of the activity of present-day S0s throughout both the broad-band PC1–PC2 spectral classifier and the conventional narrow-line BPT/WHAN ones, contrasting the different types of activity classes they define, and present an alternative diagram that exploits the concordance between WHAN and PCA demarcations. The analysis is extended to the mid-infrared, radio and X-ray wavelengths by crossmatching our core sample with data from the WISE, FIRST, XMM–Newton, and Chandra surveys. This has allowed us to carry out a thorough comparison of the most important activity diagnostics in the literature over different wavebands, discuss their similarities and differences, and explore the connections between them and with parameters related to star formation and black hole accretion. In particular, we find evidence that the bulk of nebular emission from radio and X-ray detected S0–Seyfert and LINER systems is not driven by star birth, while the dominant ionising radiation for a number of LINERs might come from post-AGB stars. These and other outcomes from the present work should be transferable to other morphologies.
Statistical properties of the population of the Galactic centre filaments – II. The spacing between filaments
Authors: Yusef-Zadeh F , Arendt R G , Wardle M , Boldyrev S , Heywood I , Cotton W , Camilo F
Published: June 2022

We carry out a population study of magnetized radio filaments in the Galactic centre using MeerKAT data by focusing on the spacing between the filaments that are grouped. The morphology of a sample of 43 groupings containing 174 magnetized radio filaments are presented. Many grouped filaments show harp-like, fragmented cometary tail-like, or loop-like structures in contrast to many straight filaments running mainly perpendicular to the Galactic plane. There are many striking examples of a single filament splitting into two prongs at a junction, suggestive of a flow of plasma along the filaments. Spatial variations in spectral index, brightness, bending, and sharpening along the filaments indicate that they are evolving on a 105−6-yr time-scale. The mean spacings between parallel filaments in a given grouping peaks at ∼16 arcsec. We argue by modeling that the filaments in a grouping all lie on the same plane and that the groupings are isotropically oriented in 3D space. One candidate for the origin of filamentation is interaction with an obstacle, which could be a compact radio source, before a filament splits and bends into multiple filaments. In this picture, the obstacle or sets the length scale of the separation between the filaments. Another possibility is synchrotron cooling instability occurring in cometary tails formed as a result of the interaction of cosmic ray driven Galactic centre outflow with obstacles such as stellar winds. In this picture, the mean spacing and the mean width of the filaments are expected to be a fraction of a parsec, consistent with observed spacing.
The hierarchical structure of galactic haloes: generalizedN-dimensional clustering with C luSTAR-ND
Authors: Oliver William H , Elahi Pascal J , Lewis Geraint F
Published: June 2022

We present C luSTAR-ND, a fast hierarchical galaxy/(sub)halo finder that produces Clustering Structure via Transformative Aggregation and Rejection in N-Dimensions. It is designed to improve upon H alo-OPTICS – an algorithm that automatically detects and extracts significant astrophysical clusters from the 3D spatial positions of simulation particles – by decreasing run-times, possessing the capability for metric adaptivity, and being readily applicable to data with any number of features. We directly compare these algorithms and find that not only does C luSTAR-ND produce a similarly robust clustering structure, it does so in a run-time that is at least 3 orders of magnitude faster. In optimizing C luSTAR-ND’s clustering performance, we have also carefully calibrated 4 of the 7 C luSTAR-ND parameters which – unless specified by the user – will be automatically and optimally chosen based on the input data. We conclude that C luSTAR-ND is a robust astrophysical clustering algorithm that can be leveraged to find stellar satellite groups on large synthetic or observational data sets.
Forecasting cosmic acceleration measurements using the Lyman-α forest
Authors: Dong Chenxing , Gonzalez Anthony , Eikenberry Stephen , Jeram Sarik , Likamonsavad Manunya , Liske Jochen , Stelter Deno , Townsend Amanda
Published: June 2022

We present results from end-to-end simulations of observations designed to constrain the rate of change in the expansion history of the Universe using the redshift drift of the Lyman-α forest absorption lines along the lines of sight towards bright quasars. For our simulations, we take Lyman-α forest lines extracted from Keck/HIRES spectra of bright quasars at z > 3, and compare the results from these real quasar spectra with mock spectra generated via Monte Carlo realizations. We use the results of these simulations to assess the potential for a dedicated observatory to detect redshift drift, and quantify the telescope and spectrograph requirements for these observations. Relative to Liske et al. (2008), two main refinements in the current work are inclusion of quasars from more recent catalogs and consideration of a realistic observing strategy for a dedicated redshift drift experiment that maximizes $\dot{v}/\sigma _{\dot{v}}$. We find that using a dedicated facility and our designed observing plan, the redshift drift can be detected at 3σ significance in 15 yr with a 25 m telescope, given a spectrograph with long-term stability with R = 50 000 and 25 per cent total system efficiency. To achieve this significance, the optimal number of targets is four quasars, with observing time weighted based upon $\dot{v}/\sigma _{\dot{v}}$ and object visibility. This optimized strategy leads to a 9 per cent decrease in the telescope diameter or a 6 per cent decrease in the required time to achieve the same S/N as for the idealized case of uniformly distributing time to the same quasars.
The past, current and future epidemiological dynamic of SARS-CoV-2
Authors: Balloux François , Tan Cedric , Swadling Leo , Richard Damien , Jenner Charlotte , Maini Mala , van Dorp Lucy
Published: June 2022

SARS-CoV-2, the agent of the COVID-19 pandemic, emerged in late 2019 in China, and rapidly spread throughout the world to reach all continents. As the virus expanded in its novel human host, viral lineages diversified through the accumulation of around two mutations a month on average. Different viral lineages have replaced each other since the start of the pandemic, with the most successful Alpha, Delta and Omicron variants of concern (VoCs) sequentially sweeping through the world to reach high global prevalence. Neither Alpha nor Delta was characterized by strong immune escape, with their success coming mainly from their higher transmissibility. Omicron is far more prone to immune evasion and spread primarily due to its increased ability to (re-)infect hosts with prior immunity. As host immunity reaches high levels globally through vaccination and prior infection, the epidemic is expected to transition from a pandemic regime to an endemic one where seasonality and waning host immunization are anticipated to become the primary forces shaping future SARS-CoV-2 lineage dynamics. In this review, we consider a body of evidence on the origins, host tropism, epidemiology, genomic and immunogenetic evolution of SARS-CoV-2 including an assessment of other coronaviruses infecting humans. Considering what is known so far, we conclude by delineating scenarios for the future dynamic of SARS-CoV-2, ranging from the good—circulation of a fifth endemic ‘common cold’ coronavirus of potentially low virulence, the bad—a situation roughly comparable with seasonal flu, and the ugly—extensive diversification into serotypes with long-term high-level endemicity.
ESTABLISHMENT OF LOCAL DIAGNOSTIC REFERENCE LEVEL FOR ROUTINE PAEDIATRIC COMPUTED TOMOGRAPHY EXAMINATIONS IN BAFOUSSAM WEST CAMEROON
Authors: Kamdem Eddy Fotso , Samba Odette Ngano , Manemo Cedric Tetchoka , Kouam Brice Bertrand Foubi , Abogo Serge , Tambe Joshua , Amougou Jean Claude Mballa , Guegang Emilienne , Zeh Odile Fernande , Moifo Boniface , Nguemgne Célestine , Nana Narcisse Fidèle Nwedjiwe , Fotue Alain Jervé
Published: June 2022

The main purpose of this study was to determine local diagnostic reference level (LDRL) for routine computed tomography (CT) examination in Bafoussam, western Cameroon. The exposure parameters and dose quantities were collected manually. This retrospective, evaluative and comparative study was conducted to determine LDRLs for routine head CT examination in Bafoussam, to optimize these procedures in the region. The 75th percentile values of the calculated volume CT dose index (CTDIvol) and dose length product (DLP) were proposed as LDRL. The sample was classified in four age groups: < 1, 1–5, 5–10 and 10–15 y. The LDRLs obtained for the four age groups were: 24 mGy and 381.32 mGy.cm, 42.5 mGy and 875.55 mGy.cm, 45.85 mGy and 939.62 mGy.cm, 57.12 mGy and 1222.3 mGy.cm, respectively. The 75th percentile CTDIvol and DLP dose values for this study are higher than international values. We propose a coordinating discussions and collaboration about patient’s and specific equipment’s change information’s, between radiologists, medical imaging technicians and medical physicist, which can reduce absorbed doses and improved medical practice in hospitals.
RECENT DEVELOPMENTS IN COMPUTERISED ANALYSIS OF THERMOLUMINESCENCE GLOW CURVES: SOFTWARE CODES, MECHANISMS AND DOSIMETRIC APPLICATIONS
Authors: Horowitz Y S , Oster L , Reshes G , Nemirovsky D , Ginzburg D , Biderman S , Bokobza Y , Sterenberg M , Eliyahu I
Published: June 2022

The computerised deconvolution of thermoluminescence glow curves into component glow peaks is discussed in detail with special emphasis on advances of the subject post 2013. A plethora of computer codes have been developed using models based on first-order kinetics, second-orders kinetics, interactive traps and continuous distributions of activation energies. The glow curves of several materials are displayed and discussed along with new and improved dosimetric applications:precision effects of heating rate, heavy charged particles, mixed field α/ϒ dosimetry, fading and dose–response linearity. Finally recommendations are made for future efforts.
LncRNA SNHG11 promotes the malignant transformation of human bronchial epithelial cells induced by beryllium sulfate
Authors: Deng Weihua , Sun Zhanbing , Chen Shaoxiong , Li Guilan , Liu Xiuli , Huang Lian , Zhang Zhaohui
Published: June 2022

Background

Beryllium and its compounds are carcinogenicity, but the mechanisms through which this occurs have yet to be clarified. Accumulating evidence exists that long noncoding RNAs (lncRNAs) play an important role in occurrence and development of cancer.

Aims and Methods

To explore the carcinogenic mechanism of beryllium, human bronchial epithelial cells (16HBE) were treated with 50 μM beryllium sulfate (BeSO4) for 45 passages (~23 weeks). The expression levels of lncRNA SNHG7, SNHG11, SNHG15, MIR22HG, GMPS, and SIK1 were detected at passage 0 (P0), 15 (P15), 25 (P25), 35 (P35), and 45 (P45).

Results

The results indicated that enhanced cell proliferation, extensive clones in soft agar, protein expressions of up-regulated matrix metalloproteinase 9 (MMP9), matrix metalloproteinase 2 (MMP2), proliferating cell nuclear antigen (PCNA), cyclin D1, and down-regulated p53 were all observed at the 45th passage in 16HBE cells. Thus, BeSO4-transformed 16HBE cells (T-16HBE) were established. Meanwhile, the study found that the expression of lncRNA SNHG11 was elevated during malignant transformation. Knockdown of SNHG11 in T-16HBE cells blocked cell proliferation, invasion, and migration, and decreased the protein levels of MMP9, MMP2, PCNA, cyclin D1, but increased p53.

Conclusions

The studies revealed that SNHG11 acts as an oncogene in the malignant transformation of 16HBE cells induced by BeSO4, which signifies progress in the study of the carcinogenic mechanism of beryllium.
SARS-CoV-2 RT-PCR positivity in relation to clinical and demographic characteristics in residents of border quarantine centres, Khyber Pakhtunkhwa, Pakistan: a prospective cohort study
Authors: Imran Muhammad , Uddin Azhar , Ambade Preshit , Khan Sajjad , Wahab Abdul , Akbar Hussain , Iqbal Zafar , Ernst Kacey
Published: June 2022

Background

As international travellers were the primary source of sever acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, border checkpoints became an important tool to isolate cases. We determined the period prevalence and SARS-CoV-2 reverse transcription polymerase chain reaction positivity in relation to clinical and demographic characteristics in healthy travellers quarantined at the Pakistan–Afghanistan border.

Methods

The study was conducted from 15 to 25 April 2020. Period prevalence was calculated and the association between positivity and individuals’ age, sex and occupation were assessed using χ2 and Mantel–Haenszel tests. Logistic regression was used to calculate adjusted odds ratios (ORs) for each age group. Time-to-event (TTE) analysis was conducted to check the difference in positivity among various groups.

Results

In a total of 708 individuals, 71 tested positive (10%). Compared with those ≤20 y of age, the sex- and occupation-adjusted odds of testing positive were less among the older age group (41–60 y; OR 0.26, p=0.008). Taxi drivers had higher odds of testing positive (OR 4.08, p<0.001). Kaplan–Meier curves and hazard ratios (0.32, p<0.01) showed that the positivity period differed significantly across the pre-symptomatic vs asymptomatic group (26 vs 14 d).

Conclusions

The cases who were likely to acquire infection through occupational exposure largely remained asymptomatic. For effective control of transmission and the emergence of new variants, testing capacities should be revamped with effective isolation measures.
Cataloging Phytophthora Species of Agriculture, Forests, Horticulture, and Restoration Outplantings in California, U.S.A.: A Sequence-Based Meta-Analysis
Authors: Bourret Tyler B. , Fajardo Sebastian N. , Frankel Susan J. , Rizzo David M.
Published: June 2022

California contains a diverse flora, and knowledge of the pathogens that threaten those plants is essential to managing their long-term health. To better understand threats to California plant health, a meta-analysis of Phytophthora detections within the state was conducted using publicly available sequences as a primary source of data rather than published records. Accessions of internal transcribed spacer (ITS) ribosomal DNA were cataloged from 800 Californian Phytophthora isolates, analyzed, and determined to correspond to 80 taxa, including several phylogenetically distinct provisional species. A number of Phytophthora taxa not previously reported from California were identified, including 20 described species. Pathways of introduction and spread were analyzed by categorizing isolates’ origins, grouped by land-use: (i) agriculture, (ii) forests and other natural ecosystems, (iii) horticulture and nurseries, or (iv) restoration outplantings. The pooled Phytophthora metacommunities of the restoration outplantings and horticulture land-use categories were the most similar, whereas the communities pooled from forests and agriculture were least similar. Phytophthora cactorum, P. pini, P. pseudocryptogea, and P. syringae were identified in all four land-use categories, while 13 species were found in three. P. gonapodyides was the most common species by number of ITS accessions and exhibited the greatest diversity of ITS haplotypes. P. cactorum, P. ramorum, and P. nicotianae were associated with the greatest number of host genera. In this analysis, the Phytophthora spp. most prevalent in California differ from those compiled from the scientific literature.
Inhibition of PECAM‐1 Significantly Delays Leukocyte Extravasation into the Subcortex Post‐Stroke
Authors: Arias Erika , Nadkarni Neil , Fang Raymond , Haynes Maureen , Batra Ayush , Muller William , Sullivan David
Published: June 2022

Background and Purpose

Current therapies for ischemic stroke focus on reperfusion but do not address the acute inflammatory response that results in significant reperfusion injury. To advance future therapies, a thorough understanding of the precise spatiotemporal underpinnings of leukocyte extravasation and infiltration is necessary. We describe the evolution of the inflammatory response in a mouse transient middle cerebral artery occlusion (tMCAO) stroke model at several time points after reperfusion and the modulation of this response with PECAM blockade.

Methods

The transient Middle Cerebral Artery Occlusion model (90 minutes of ischemia followed by reperfusion) was used to simulate large vessel occlusion stroke and recanalization. We used wide field and confocal immunofluorescence microscopy to examine the exact distribution of neutrophils with close examination of the leukocyte position with regard to the brain vasculature and the perivascular space. Flow cytometry of single cell suspensions was used to confirm cell identity at different time points post‐stroke.

Results

Large ischemic strokes involving both the subcortex and cortex (over 20% of the ischemic hemisphere) were induced in mice. At 12 and 24 hours, leukocyte recruitment and extravasation was primarily localized to the cortical surface. This contrasts with other organs where there is considerable migration of neutrophils deep into the inflamed tissue by 24 hours. Flow cytometry showed at 24 hours a majority of leukocytes were neutrophils. Over 48 to 72 hours, leukocytes were increasingly found deeper into the subcortex. Throughout the infarct (determined with triphenyl tetrazolium chloride staining), leukocyte recruitment was not uniform but rather organized in clusters. Disrupting leukocyte diapedesis with PECAM function‐blocking monoclonal antibody restricted leukocytes to within 500 microns of the surface when compared to control; and this was still evident at 72 hours (n=3 mice per group, p<0.01, Control 46% ± 4.0 %; PECAM‐1 Ab 62% ± 5.0%). High‐resolution wide‐field microscopy confirmed inhibition of TEM by PECAM‐1 blockade at 24 hours. Flow cytometry showed approximately equal numbers of monocytes and neutrophils at 72 hours.

Conclusions

Our findings demonstrate that leukocyte infiltration into a stroke evolves over several days following reperfusion. The use of PECAM blockade modulates the natural progression of leukocytes into the infarcted stroke bed. A better understanding of leukocyte spatiotemporal infiltration and its regulators could help inform the next generation of therapeutic interventions.
Foundational Approaches to Advancing Hearing Health Equity: A Primer in Social Epidemiology
Authors: Nieman Carrie L. , Suen Jonathan J. , Dean Lorraine T. , Chandran Aruna
Published: June 2022

Hearing health is inextricably linked to factors beyond biology. Social, demographic, environmental, geographic, and historical influences affect hearing health, but these factors are often unmeasured within traditional biological, clinical, and epidemiological studies of hearing health. With increasing recognition of hearing health over the life course as a public health priority, there is also a growing understanding of existing hearing health inequities at the individual, community, national, and global levels. To make progress in addressing these inequities, public health disciplines, such as social epidemiology, can provide valuable frameworks. With a focus on integrating the biological and functional with social and structural factors influencing health, social epidemiology provides key concepts and approaches for filling existing research and practice gaps. In this review, we introduce the discipline of social epidemiology and its associated concepts to inspire greater cross-disciplinary collaboration for the ultimate goal of advancing hearing health equity.
The Psychological and Somatic Consequences of Digital Amputation
Authors: Skoff Hillel , Skoff Hannah
Published: June 2022

Background:

Major limb amputation is a devastating potential outcome of trauma, tumor, or disease. Much has been written about the physical, functional, economic, and psychological consequences of major limb loss. In contradistinction, considerably less has been written concerning the consequences of “minor” limb loss, specifically single partial digit amputations. Are minor limb (partial single digit) amputations associated with symptoms of psychological disorder similar to those reported for major limb amputations?

Methods:

We conducted a clinical research study through interview and examination of 25 adult patients (average age: 45 years) who had suffered a single partial digit amputation to determine if symptoms of depression, anxiety, anger, or posttraumatic stress disorder newly occurred, and if such symptoms correlated with the surgical outcome. Questionnaires for Quick-DASH, Michigan Hand Score, and Diagnostic and Statistical Manual of Mental Disorders-5 Psychological Profile testing were completed.

Results:

All but one of the patients suffered from psychological symptoms for a minimum of 3 months. Symptom resolution time averaged 6 months for seven of the 25 patients. For 18 of the 25 patients, both psychological disturbance and neuroma pain were ongoing. The Psychological Profile scores suggesting pathology were inversely related to the scores on the Quick-Dash and Michigan Hand (somatic) questionnaires indicating wellness (P < 0.03).

Conclusions:

(1) Even minor partial amputations of single digits can trigger significant psychological disturbance; the study hypothesis is validated. (2) Psychological and somatic outcomes are directly correlative. (3) Mitigating neuroma pain and verbally offering psychological support services early in the postamputation period should improve the clinical outcome of digital amputations.
Destigmatizing and Democratizing Postpartum Care: A “Black Woman-Person First” Approach
Authors: SCOTT KAREN A. , DAVIS DÁNA-AIN
Published: June 2022

Optimizing postpartum care highlights the need for care coordination, enhancement, and expansion of health care services after childbirth. Yet the prioritization of disease surveillance, management, and mitigation during birth and beyond within the American College of Obstetrics and Gynecology facilitates the medicalization and pathologization of Black bodies, voices, and power. Thus, we offer the Building and Bridging Black Futures Beyond Birth Model: A 12-Step Black Woman-Person First Approach, as a more humane and holistic model of culturally affirming and clinically responsive care. Destigmatizing and democratizing care bridges the gap between intent and impact in postpartum care optimization, particularly for Black women, girls, and gender expansive people and their communities.
Whole-Blood and Peripheral Mononuclear Cell Transcriptional Response to Prolonged Altitude Exposure in Well-Trained Runners
Authors: Sutehall Shaun , Malinsky Fernanda , Shurlock Jonathan , Wang Guan , Bosch Andrew , Pitsiladis Yannis P.
Published: June 2022

Background:

Recombinant human erythropoietin (rHuEpo) abuse by athletes threatens the integrity of sport. Due to the overlap in physiological response to rHuEpo and altitude exposure, it remains difficult to differentiate changes in hematological variables caused by rHuEpo or altitude, and therefore, other molecular methods to enhance anti-doping should be explored.

Objective:

To identify the hematological and transcriptomic response to prolonged altitude exposure typical of practices used by elite athletes.

Design:

Longitudinal study.

Setting:

University of Cape Town and Altitude Training Centre in Ethiopia.

Participants and Intervention:

Fourteen well-trained athletes sojourned to an altitude training camp in Sululta, Ethiopia (∼2400-2500 m above sea level) for 27 days. Blood samples were taken before arrival, 24 hours, and 9, 16, and 24 days after arrival at altitude in addition to 24 hours and 6, 13, and 27 days upon return to sea level.

Main Outcome Measures:

Blood samples were analyzed for hemoglobin concentration, hematocrit, and reticulocyte percentage. The transcriptomic response in whole blood and peripheral blood mononuclear cells (PBMC) were analyzed using gene expression microarrays.

Results:

A unique set of 29 and 10 genes were identified to be commonly expressed at every altitude time point in whole blood and PBMC, respectively. There were no genes identified upon return to sea level in whole blood, and only one gene within PBMC.

Conclusions:

The current study has identified a series of unique genes that can now be integrated with genes previously validated for rHuEpo abuse, thereby enabling the differentiation of rHuEpo from altitude exposure.
Sexually Transmitted Infection Testing, Prevalence, and Treatment Among Individuals Receiving HIV Preexposure Prophylaxis Within an Integrated Healthcare Delivery System
Authors: Schmidt Mark A. , Dickerson John F. , O'Keeffe-Rosetti Maureen C. , Salas Suzanne B. , Donald Judy L. , Tao Guoyu
Published: June 2022

Background

Initial and follow-up sexually transmitted infection (STI) and human immunodeficiency virus (HIV) testing are recommended when taking HIV preexposure prophylaxis (PrEP). We assessed STI services before and after PrEP initiation among persons 18 years or older.

Methods

We conducted this retrospective cohort study at a US integrated healthcare delivery system. We measured HIV/STI testing rates, STI prevalence and treatment at 3 time points: (1) at PrEP initiation, (2) at 120 days, and (3) at 210 days.

Results

Of 685 PrEP initiators, 67.2% continued PrEP use at 120 days and 49.5% at 210 days. Of PrEP users, HIV and STI testing were greater than 85% and greater than 80%, respectively, at all 3 time points. Prevalence for any chlamydia, rectal chlamydia, and any gonorrhea, rectal gonorrhea, or pharyngeal gonorrhea was always high at the 120 days and 210 days (eg, 6.9%, 10.5%, 6.7%, 5.0%, and 5.2%, respectively, at the 120 days for continuous PrEP users). Over 90% of all individuals who tested positive for chlamydia and gonorrhea received antibiotic pharmacy fills within 7 days at 120 and 210 days. Monthly PrEP-related pharmacy cost was about $2259 to $2659. The proportion of the total medical cost that was PrEP-related pharmacy was about 82% for PrEP continuous users.

Conclusions

Although HIV/STI testing rates were high, they can still be improved during HIV PrEP management. High STI prevalence after PrEP initiation in this study suggests that patients taking PrEP are at risk of acquiring an STI. Interventions to improve STI services during PrEP management are continuously needed.
Unravelled multilevel transformation networks for predicting sparsely observed spatio-temporal dynamics
Authors: Saha Priyabrata , Mukhopadhyay Saibal
Published: June 2022

In this paper, we address the problem of predicting complex, nonlinear spatio-temporal dynamics when available data are recorded at irregularly spaced sparse spatial locations. Most of the existing deep learning models for modelling spatio-temporal dynamics are either designed for data in a regular grid or struggle to uncover the spatial relations from sparse and irregularly spaced data sites. We propose a deep learning model that learns to predict unknown spatio-temporal dynamics using data from sparsely-distributed data sites. We base our approach on the radial basis function (RBF) collocation method which is often used for meshfree solution of partial differential equations. The RBF framework allows us to unravel the observed spatio-temporal function and learn the spatial interactions among data sites on the RBF-space. The learned spatial features are then used to compose multilevel transformations of the raw observations and predict its evolution in future time steps. We demonstrate the advantage of our approach using both synthetic and real-world climate data.

This article is part of the theme issue ‘Data-driven prediction in dynamical systems’.