bluuebunny/crossref_metadata_2025 huggingface dataset:
The Burden Cancer-Related Deaths Attributable to High Body Mass Index in a Gulf Cooperation Council: Results from the Global Burden of Disease Study 2019
Authors: Ramadan Majed , Bajunaid Rbab M. , Kazim Sereen , Alhusseini Noara , Al-Shareef Ali , ALSaleh Nourah Mohammed
Published: May 2024

Background

BMI has been reported to be a major risk factor for the increased burden of several diseases. This study explores the burden of cancer linked to high body mass index (BMI) in Gulf Cooperation Council (GCC) countries and assesses the correlation with Socio-demographic Index (SDI).

Method

Using Global burden of disease (GBD) 2019 data, the authors quantified cancer burden through mortality, DALYs, age standardized mortality rate (ASMR), and age standardized DALYs rate (ASDR) across sexes, countries, cancer types, and years. Spearman’s correlation tested ASMR against SDI. The authors estimated 95% uncertainty limits (UIs) for population attribution fraction (PAFs).

Results

Between 1990 and 2019, all six GCC countries showed increased number of the overall cancer-related deaths (398.73% in Bahrain to 1404.25% in United Arab Emirates), and DALYs (347.38% in Kuwait, to 1479.35% in United Arab Emirates) reflecting significant increasing in deaths, and burden cancer attributed to high BMI. In 2019, across GCC countries, pancreatic, uterine, and kidney cancer accounted for 87.91% of the total attributable deaths associated with high BMI in females, whereas in male, colon and rectum cancer alone accounted for 26% of all attributable deaths associated with high BMI.

Conclusion

The study highlights the significant impact of high BMI on cancer burden in GCC countries. Moreover, the study identifies specific cancers, such as pancreatic, uterine, and kidney cancer in females, and colon and rectum cancer in males, as major contributors to attributable deaths, urging targeted prevention strategies at reducing weight and encouraging physical activity could greatly lessen the impact of diseases in the GCC countries.
The pandemic and working mothers: perspectives from American and South African working mothers
Authors: Rosen Lisa H. , Scott Shannon R. , Shukla Roshni , Poe Darian , Ahmed Shazia
Published: May 2024

The purpose of the current study was to examine the effects of the COVID-19 pandemic on working mothers from the United States and South Africa. This study sought to highlight both cross-cultural similarities and differences. American and South African working mothers took part in online interviews. Eighteen participants were included in the sample, ten participants lived in the United States, while eight participants currently resided in South Africa. The age range of the participants’ children was from 0 to 21 years old. Each participant met individually with the researcher and answered a series of open-ended questions regarding their experiences of being a working mother during the COVID-19 pandemic. Researchers analyzed interviews using thematic analysis. Common themes emerged in the interviews with both American and South African working mothers. Mothers in the United States and South Africa both noted the need for flexible work schedules as well as expressed difficulty balancing roles as mothers and professionals during the pandemic. Increased motivation and resiliency as well as lack of representation of women leaders were themes specific to South African mothers in this study. Conversely, feelings of decreased interest in pursuing leadership opportunities was a theme specific to American mothers in this study. The current study adds to the growing body of literature documenting the effect of the pandemic on working mothers, and builds on this research by including a qualitative component to gain knowledge from working mothers themselves. The narratives collected in the current study highlight commonalities as well as differences in the experiences of working mothers in the United States and South Africa, which holds important implications for how workplaces and the broader community can best attend to the unique needs of working mothers.
The CDC’s “Solve the Outbreak” game: a preliminary debriefing study with students enrolled in an undergraduate epidemiology course
Authors: Seitz Christopher M. , Lennon Jeffrey L. , Orsini Muhsin Michael , Elliot Lauren , Lloyd Margaret , Findley Fin
Published: May 2024

Purpose

This study explored undergraduate college students' opinions of the CDC’s online “Solve the Outbreak” (StO) game, a recommended aid in higher education for teaching concepts epidemiological outbreak investigations.

Methods

The study was conducted as a game debriefing, in which 16 students enrolled in an undergraduate-level epidemiology course were interviewed to receive feedback about their experiences of StO after completing the game.

Results

A total of four common themes emerged from the interveiews as framed by the Persuasive Game Design model: (1) Enjoying the Game World, (2) Cheating the Game’s Elements, (3) Suggesting Improvements to the Game’s Elements, and (4) Inverted Transfer Effect.

Conclusions

Students reported that they enjoyed playing StO, and that it was effective for applying their knowledge of epidemiology course content on the process of investigating outbreaks. However, students found ways to cheat the game’s answers, and they recommended ways that the game’s elements could be improved. Instructors should consider using StO as a tool for teaching their students about investigating infectious outbreaks. Designers of the game should consider adapting the game based on students’ constructive feedback.
Irrigation with Artificial Intelligence: Problems, Premises, Promises
Authors: Wei Hanyu , Xu Wen , Kang Byeong , Eisner Rowan , Muleke Albert , Rodriguez Daniel , deVoil Peter , Sadras Victor , Monjardino Marta , Harrison Matthew Tom
Published: May 2024

Protagonists allege that artificial intelligence (AI) is revolutionising contemporaneous mindscapes. Here, we authoritatively review the status quo of AI and machine learning application in irrigated agriculture, evaluating the potential of, and challenges associated with, a wide range of existential AI approaches. We contend that aspiring developers of AI irrigation systems may benefit from human-centred AI, a nascent algorithm that captures diverse end-user views, behaviours and actions, potentially facilitating refinement of proposed systems through iterative stakeholder feedback. AI-guided human–machine collaboration can streamline integration of user needs, allowing customisation towards situational farm management adaptation. Presentation of big data in intuitive, legible and actionable forms for specialists and laypeople also urgently requires attention: here, AI-explainable interpretability may help harness human expertise, enabling end-users to contribute their experience within an AI pipeline for bespoke outputs. Transfer learning holds promise in contextualising place-based AI to agroecological regions, production systems or enterprise mixes, even with limited data inputs. We find that the rate of AI scientific and software development in recent times has outpaced the evolution of adequate legal and institutional regulations, and often social, moral and ethical license to operate, revealing consumer issues associated with data ownership, legitimacy and trust. We opine that AI has great potential to elicit sustainable outcomes in food security, social innovation and environmental stewardship, albeit such potential is more likely to be realised through concurrent development of appropriate ethical, moral and legal dimensions.
Spatially evolving cascades in wall turbulence with and without interface
Authors: Cimarelli A. , Boga G. , Pavan A. , Costa P. , Stalio E.
Published: May 2024

Direct numerical simulations of channel flow and temporal boundary layer at a Reynolds number

$Re_{\tau } = 1500$

are used to assess the scale-by-scale mechanisms of wall turbulence. From the peak of turbulence production embedded at the small scales of the near-wall region, spatially ascending reverse cascades are generated that move through self-similar eddies growing in size with the wall distance. These fluxes are followed by spatially ascending forward cascades through detached eddies thus reaching sufficiently small scales where eventually scale energy is dissipated. This phenomenology is shared by both boundary layer and channel flow and is recognized as a robust physical feature characterizing wall turbulence in general. Specific features related to the flow configuration are indeed identified in the outer region. In particular, the central region of channels is characterized by a generalized Richardson energy cascade where large scales are in equilibrium with small scales at different wall distances through a combined forward cascade and spatial flux. On the contrary, the interface region of boundary layers is characterized by an almost two-dimensional physics where spatially ascending reverse cascades sustain long and wide interface structures with a forward cascade that survives only in the wall-normal scales. The overall scenario consists in a variety of scale motions that while protruding from the turbulent core towards the external region, squeeze at the interface thus sustaining vertical shear in a thin layer. The observed multidimensional physics sheds light on the complex interactions between outer entrainment and near-wall self-sustaining mechanisms with possible repercussions for theories.
THE SET OF ELEMENTARY TENSORS IS WEAKLY CLOSED IN PROJECTIVE TENSOR PRODUCTS
Authors: PETITJEAN COLIN
Published: May 2024

We prove that the set of elementary tensors is weakly closed in the projective tensor product of two Banach spaces. As a result, we answer a question of Rodríguez and Rueda Zoca [‘Weak precompactness in projective tensor products’, Indag. Math. (N.S.)35(1) (2024), 60–75], proving that if

$(x_n) \subset X$

and

$(y_n) \subset Y$

are two weakly null sequences such that

$(x_n \otimes y_n)$

converges weakly in

$X \widehat {\otimes }_\pi Y$

, then

$(x_n \otimes y_n)$

is also weakly null.
The determinants of proxy treadmilling in evolutionary models of reliable signals
Authors: Harris Keith D.
Published: May 2024

Identifying the conditions of proxy treadmilling is crucial for determining whether reliable signals can persist over time. I present a framework that maps evolutionary models of reliable signals according to their assumptions regarding the effects of Goodhart's law. This framework can explain the contrasting outcomes of different modelling approaches, and identify in which models proxy treadmilling is expected to occur.
Proxy failure as a feature of adaptive control systems
Authors: Pernu Tuomas K.
Published: May 2024

The analysis of John et al. is lacking a fully general account of proxy failure. It is here proposed that proxy failure can be understood as a feature of all adaptive control systems. Whether proxies “fail” or “succeed” depends on the more encompassing view one can adopt for observing such systems.
Proxy failure and poor measurement practices in psychological science
Authors: Higgins Wendy C. , Gillett Alexander J. , Kaplan David M. , Ross Robert M.
Published: May 2024

We argue that proxy failure contributes to poor measurement practices in psychological science and that a tradeoff exists between the legibility and fidelity of proxies whereby increasing legibility can result in decreased fidelity.
Regulator and agent sophistication as an explanation-generating engine for proxy failure dynamics
Authors: Moldoveanu Mihnea
Published: May 2024

I consider the dynamics of regulator–agent interactions in situations in which there are significant mismatches in their abilities to discern and register information and calculate and act upon successful inferences.
Proxies, heuristics, and goal alignment
Authors: Burns Bruce D.
Published: May 2024

Decision-making heuristics rely on proxies so the elements of heuristics appear to map well to the elements of proxies identified by John et al. However, unlike proxy failure, heuristics do not fail because of feedback. This may be because for successful heuristics the goals of regulators and agents are aligned, but this is not the case for proxy failure.
Behavioral proxies compete by the time courses of their rewards, including endogenous rewards
Authors: Ainslie George
Published: May 2024

Natural selection is slow, so behavioral goals must be based on patterns of reward. Addictions are rewarded in the same way as adaptive choice, so they can be distinguished only by their time course. In addition, the reward process is more plastic than is generally recognized, so abstract goals are shaped by the “legibility” of their proxies.
Striatal brain volume linked to severity of substance use in high-risk incarcerated youth
Authors: Anderson Nathaniel E. , Maurer J. Michael , Stephenson David , Harenski Keith , Caldwell Michael , Van Rybroek Greg , Kiehl Kent A.
Published: May 2024

Substance use disorders among juveniles are a major public health concern and are often intertwined with other psychosocial risk factors including antisocial behavior. Identifying etiological risks and mechanisms promoting substance use disorders remains a high priority for informing more focused interventions in high-risk populations. The present study examined brain gray matter structure in relation to substance use severity among n = 152 high-risk, incarcerated boys (aged 14–20). Substance use severity was positively associated with gray matter volume across several frontal/striatal brain regions including amygdala, pallidum, putamen, insula, and orbitofrontal cortex. Effects were apparent when using voxel-based-morphometric analysis, as well as in whole-brain, data-driven, network-based approaches (source-based morphometry). These findings support the hypothesis that elevated gray matter volume in striatal reward circuits may be an endogenous marker for vulnerability to severe substance use behaviors among youth.
Prospective associations of prenatal stress with child behavior: Moderation by the early childhood caregiving environment
Authors: Rinne Gabrielle R. , Podosin Mallory , Mahrer Nicole E. , Shalowitz Madeleine U. , Ramey Sharon Landesman , Dunkel Schetter Christine
Published: May 2024

Fetal exposure to prenatal stress can increase risk for psychopathology but postnatal caregiving may offset risk. This study tests whether maternal sensitivity and the home environment during early childhood modify associations of prenatal stress with offspring behavior in a sample of 127 mother–child pairs (n = 127). Mothers reported on perceived stress during pregnancy. Maternal sensitivity was rated by coders during a parent–child free play task when children were 4 years old. One year later, mothers reported on the home environment, child internalizing and externalizing behaviors, and children completed an assessment of inhibitory control. As hypothesized, the early childhood caregiving environment modified associations of prenatal stress with child behavior. Specifically, prenatal stress was associated with more internalizing behaviors at lower levels of maternal sensitivity and in home environments that were lower in emotional support and cognitive stimulation, but not at mean or higher levels. Furthermore, prenatal stress was associated with lower inhibitory control only at lower levels of maternal sensitivity, but not at higher levels. Maternal sensitivity and an emotionally supportive and cognitively stimulating home environment in early childhood may be important factors that mitigate risk for mental health problems among children exposed to prenatal stress.
Sweden’s Peculiar Adoption of Proportional Representation: The Overlooked Effects of Time and History
Authors: Kreuzer Marcus , Neely Runa
Published: May 2024

Sweden’s adoption of proportional representation (PR) is interesting because it involved static structural and institutional factors, well captured by variance-based left-threat thesis, and four temporal factors—sequencing, timing, historical change, and duration—that historical case studies highlight. We integrate these two sets of factors. We fuse the more static, temporally homogeneous world created by the left-threat thesis, that is well suited to explain cross-sectional variations, with the more dynamic, temporally heterogenous world presumed by the case studies that is attuned to temporal processes. It illustrates how comparative historical analysis (CHA) can translate temporal anomalies into generalizable temporal mechanisms and how nested analysis, together with causal graphs, provide helpful tools for updating theories. We ultimately employ an abductive approach that evaluates evidence not just for its inferential leverage of confirming theories but also for its inductive potential to generate new, more test-worthy hypotheses.
Rainband‐Occurrence Probability in Northern Hemisphere Tropical Cyclones by Synthetic Aperture Radar Imagery
Authors: Zheng Gang , Jiang Han , Wu Liang , Li Xiaofeng , Zhou Lizhang , Wu Qiaoyan , Chen Peng , Ren Lin
Published: May 2024

Rainbands are essential to tropical cyclones (TCs), significantly affecting TC structure and intensity change. High‐resolution synthetic aperture radar (SAR) imagery can capture the footprints of rainbands caused by rain‐induced sea surface roughness modification. Using 464 SAR TC images, we investigated the rainband‐occurrence probability of TCs under different hemispheres, local times (LTs), intensities, and ocean basins. Results show that the rainband‐occurrence probability is highest in the downshear‐left quadrant for Northern Hemisphere TCs (downshear‐right quadrant for Southern Hemisphere TCs). For Northern Hemisphere TCs, the rainband‐occurrence probability is overall higher in the early morning (LT), and the peak region of rainband‐occurrence probability appears farther from the TC center in the evening (LT). Compared with weak TCs, the rainband‐occurrence probability becomes higher for strong TCs in the Northern Hemisphere. Furthermore, TCs have a higher rainband‐occurrence probability in the Northwest Pacific than in the North Atlantic and Northeast Pacific.
Enhanced potency of an IgM-like nanobody targeting conserved epitope in SARS-CoV-2 spike N-terminal domain
Authors: Liu Bo , Liu Honghui , Han Pu , Wang Xiaoyun , Wang Chunmei , Yan Xinxin , Lei Wenwen , Xu Ke , Zhou Jianjie , Qi Jianxun , Fan Ruiwen , Wu Guizhen , Tian Wen-xia , Gao George F. , Wang Qihui
Published: May 2024

Almost all the neutralizing antibodies targeting the receptor-binding domain (RBD) of spike (S) protein show weakened or lost efficacy against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged or emerging variants, such as Omicron and its sub-variants. This suggests that highly conserved epitopes are crucial for the development of neutralizing antibodies. Here, we present one nanobody, N235, displaying broad neutralization against the SARS-CoV-2 prototype and multiple variants, including the newly emerged Omicron and its sub-variants. Cryo-electron microscopy demonstrates N235 binds a novel, conserved, cryptic epitope in the N-terminal domain (NTD) of the S protein, which interferes with the RBD in the neighboring S protein. The neutralization mechanism interpreted via flow cytometry and Western blot shows that N235 appears to induce the S1 subunit shedding from the trimeric S complex. Furthermore, a nano-IgM construct (MN235), engineered by fusing N235 with the human IgM Fc region, displays prevention via inducing S1 shedding and cross-linking virus particles. Compared to N235, MN235 exhibits varied enhancement in neutralization against pseudotyped and authentic viruses in vitro. The intranasal administration of MN235 in low doses can effectively prevent the infection of Omicron sub-variant BA.1 and XBB in vivo, suggesting that it can be developed as a promising prophylactic antibody to cope with the ongoing and future infection.
Pirh2 modulates the mitochondrial function and cytochrome c-mediated neuronal death during Alzheimer’s disease
Authors: Singh Abhishek , Tiwari Shubhangini , Singh Sarika
Published: May 2024

Pirh2 is an E3 ubiquitin ligase known to regulate the DNA damage responses through ubiquitylation of various participating signaling factors. DNA damage is a key pathological contributor to Alzheimer’s disease (AD), therefore, the role of Pirh2 was investigated in streptozotocin and oligomer Aβ1–42 induced rodent experimental model of AD. Pirh2 protein abundance increased during AD conditions, and transient silencing of Pirh2 inhibited the disease-specific pathological markers like level of p-Tau, βamyloid, acetylcholinesterase activity, and neuronal death. Biochemically, Pirh2 silencing significantly attenuated the oxidative stress, depleted mitochondrial membrane potential, cytochrome c translocation from mitochondria to cytosol, and depleted mitochondrial complex-I activity, and ATP level. Pirh2 silencing also inhibited the altered level of VDAC1, hsp75, hexokinase1, t-Bid, caspase-9, and altered level of apoptotic proteins (Bcl-2, Bax). MALDI-TOF/TOF, co-immunoprecipitation, and UbcH13-linked ubiquitylation assay confirmed the interaction of Pirh2 with cytochrome c and the role of Pirh2 in ubiquitylation of cytochrome c, along with Pirh2-dependent altered proteasome activity. Additionally, Pirh2 silencing further inhibited the translocation of mitochondrion-specific endonuclease G and apoptosis-inducing factors to the nucleus and DNA damage. In conclusion, findings suggested the significant implication of Pirh2 in disease pathogenesis, particularly through impaired mitochondrial function, including biochemical alterations, translocation of cytochrome c, endonuclease G and apoptosis-inducing factor, DNA damage, and neuronal apoptosis.
Tumors cells with mismatch repair deficiency induce hyperactivation of pyroptosis resistant to cell membrane damage but are more sensitive to co-treatment of IFN-γ and TNF-α to PANoptosis
Authors: Li Huiyan , Ni Hengli , Li Ying , Zhou Aijun , Qin Xiaokang , Li Yuqing , Che Liheng , Mo Hui , Qin Chao , Li Jianming
Published: May 2024

Hypermutated neoantigens in cancers with DNA mismatch repair deficiency (dMMR) are prerequisites for favorable clinical responses to immune-checkpoint blockade (ICB) therapy. However, TMB is not significantly associated with favorable prognosis from Preclinical and clinical studies. It implies that except for TMB, other mechanisms should be needed to contribute to successful cancer immunotherapy. We found that the hyperactivation of PANoptotic effective molecules in dMMR tumor cells caused cell membrane damage, induced ESCRT-mediated membrane repair, and protected tumor cells from the damage caused by Triton X-100, while DNA mismatch repair proficient (pMMR) tumor cells were sensitive to Triton X-100 mediating cell membrane damage due to the lack of ESCRT-mediated membrane repair. There was hyperactivation of GSDMD, GSDME, and p-MLKL in dMMR tumor cells. Co-treatment of IFN-γ and TNF-α induced rapid death of dMMR tumor cells by inducing PANoptosis including pyroptosis, apoptosis, and no necrosis. pMMR tumor cells had defects in the PANoptosis pathway and were resistant to co-treatment of IFN-γ and TNF-α. In conclusion, we can activate immune cells to release IFN-γ and TNF-α to overcome resistance to ICB treatment.
Methylation of ESCRT-III components regulates the timing of cytokinetic abscission
Authors: Richard Aurélie , Berthelet Jérémy , Judith Delphine , Advedissian Tamara , Espadas Javier , Jannot Guillaume , Amo Angélique , Loew Damarys , Lombard Berangere , Casanova Alexandre G. , Reynoird Nicolas , Roux Aurélien , Berlioz-Torrent Clarisse , Echard Arnaud , Weitzman Jonathan B. , Medjkane Souhila
Published: May 2024

Abscission is the final stage of cytokinesis, which cleaves the intercellular bridge (ICB) connecting two daughter cells. Abscission requires tight control of the recruitment and polymerization of the Endosomal Protein Complex Required for Transport-III (ESCRT-III) components. We explore the role of post-translational modifications in regulating ESCRT dynamics. We discover that SMYD2 methylates the lysine 6 residue of human CHMP2B, a key ESCRT-III component, at the ICB, impacting the dynamic relocation of CHMP2B to sites of abscission. SMYD2 loss-of-function (genetically or pharmacologically) causes CHMP2B hypomethylation, delayed CHMP2B polymerization and delayed abscission. This is phenocopied by CHMP2B lysine 6 mutants that cannot be methylated. Conversely, SMYD2 gain-of-function causes CHMP2B hypermethylation and accelerated abscission, specifically in cells undergoing cytokinetic challenges, thereby bypassing the abscission checkpoint. Additional experiments highlight the importance of CHMP2B methylation beyond cytokinesis, namely during ESCRT-III-mediated HIV-1 budding. We propose that lysine methylation signaling fine-tunes the ESCRT-III machinery to regulate the timing of cytokinetic abscission and other ESCRT-III dependent functions.
Structural and dynamic insights into the activation of the μ-opioid receptor by an allosteric modulator
Authors: Kaneko Shun , Imai Shunsuke , Uchikubo-Kamo Tomomi , Hisano Tamao , Asao Nobuaki , Shirouzu Mikako , Shimada Ichio
Published: May 2024

G-protein-coupled receptors (GPCRs) play pivotal roles in various physiological processes. These receptors are activated to different extents by diverse orthosteric ligands and allosteric modulators. However, the mechanisms underlying these variations in signaling activity by allosteric modulators remain largely elusive. Here, we determine the three-dimensional structure of the μ-opioid receptor (MOR), a class A GPCR, in complex with the Gi protein and an allosteric modulator, BMS-986122, using cryogenic electron microscopy. Our results reveal that BMS-986122 binding induces changes in the map densities corresponding to R1673.50 and Y2545.58, key residues in the structural motifs conserved among class A GPCRs. Nuclear magnetic resonance analyses of MOR in the absence of the Gi protein reveal that BMS-986122 binding enhances the formation of the interaction between R1673.50 and Y2545.58, thus stabilizing the fully-activated conformation, where the intracellular half of TM6 is outward-shifted to allow for interaction with the Gi protein. These findings illuminate that allosteric modulators like BMS-986122 can potentiate receptor activation through alterations in the conformational dynamics in the core region of GPCRs. Together, our results demonstrate the regulatory mechanisms of GPCRs, providing insights into the rational development of therapeutics targeting GPCRs.
Identifying regions of importance in wall-bounded turbulence through explainable deep learning
Authors: Cremades Andrés , Hoyas Sergio , Deshpande Rahul , Quintero Pedro , Lellep Martin , Lee Will Junghoon , Monty Jason P. , Hutchins Nicholas , Linkmann Moritz , Marusic Ivan , Vinuesa Ricardo
Published: May 2024

Despite its great scientific and technological importance, wall-bounded turbulence is an unresolved problem in classical physics that requires new perspectives to be tackled. One of the key strategies has been to study interactions among the energy-containing coherent structures in the flow. Such interactions are explored in this study using an explainable deep-learning method. The instantaneous velocity field obtained from a turbulent channel flow simulation is used to predict the velocity field in time through a U-net architecture. Based on the predicted flow, we assess the importance of each structure for this prediction using the game-theoretic algorithm of SHapley Additive exPlanations (SHAP). This work provides results in agreement with previous observations in the literature and extends them by revealing that the most important structures in the flow are not necessarily the ones with the highest contribution to the Reynolds shear stress. We also apply the method to an experimental database, where we can identify structures based on their importance score. This framework has the potential to shed light on numerous fundamental phenomena of wall-bounded turbulence, including novel strategies for flow control.
Coherent electric field control of orbital state of a neutral nitrogen-vacancy center
Authors: Kurokawa Hodaka , Wakamatsu Keidai , Nakazato Shintaro , Makino Toshiharu , Kato Hiromitsu , Sekiguchi Yuhei , Kosaka Hideo
Published: May 2024

The coherent control of the orbital state is crucial for realizing the extremely-low power manipulation of the color centers in diamonds. Herein, a neutrally-charged nitrogen-vacancy center, NV0, is proposed as an ideal system for orbital control using electric fields. The electric susceptibility in the ground state of NV0 is estimated, and found to be comparable to that in the excited state of NV−. Also, the coherent control of the orbital states of NV0 is demonstrated. The required power for orbital control is three orders of magnitude smaller than that for spin control, highlighting the potential for interfacing a superconducting qubit operated in a dilution refrigerator.
A multifunctional smart field-programmable radio frequency surface
Authors: Li Tianzhi , Yu Yang , Liu Yutong , Unnithan Ranjith R. , McDermott Ryan J. , Schreurs Dominique , Evans Robin , Skafidas Efstratios
Published: May 2024

Antennas that can operate across multiple communication standards have remained a challenge. To address these limitations, we propose a Field-Programmable Radio Frequency Surface (FPRFS), which is based on manipulating current flow on its surface to achieve desirable RF characteristics. In this work, we demonstrate that substantial enhancements in radiation efficiency can be achieved while preserving the high reconfigurability of antenna structures implemented on the FPRFS. This is accomplished by utilizing an asymmetric excitation, directing the excitation to the low-loss contiguous surface, and dynamically manipulating the imaged return current on a segmented ground plane by switches. This important insight allows for adaptable antenna performance that weakly depends on the number of RF switches or their loss. We experimentally validate that FPRFS antennas can achieve efficiencies comparable to traditionally implemented antenna counterparts. This permits the FPRFS to be effectively utilized as a productive antenna and impedance-matching network with real-time reconfigurability.
Lead-free dual-frequency ultrasound implants for wireless, biphasic deep brain stimulation
Authors: Wang Qian , Zhang Yusheng , Xue Haoyue , Zeng Yushun , Lu Gengxi , Fan Hongsong , Jiang Laiming , Wu Jiagang
Published: May 2024

Ultrasound-driven bioelectronics could offer a wireless scheme with sustainable power supply; however, current ultrasound implantable systems present critical challenges in biocompatibility and harvesting performance related to lead/lead-free piezoelectric materials and devices. Here, we report a lead-free dual-frequency ultrasound implants for wireless, biphasic deep brain stimulation, which integrates two developed lead-free sandwich porous 1-3-type piezoelectric composite elements with enhanced harvesting performance in a flexible printed circuit board. The implant is ultrasonically powered through a portable external dual-frequency transducer and generates programmable biphasic stimulus pulses in clinically relevant frequencies. Furthermore, we demonstrate ultrasound-driven implants for long-term biosafety therapy in deep brain stimulation through an epileptic rodent model. With biocompatibility and improved electrical performance, the lead-free materials and devices presented here could provide a promising platform for developing implantable ultrasonic electronics in the future.
Rapid and on-site wireless immunoassay of respiratory virus aerosols via hydrogel-modulated resonators
Authors: Li Xin , Sun Rujing , Pan Jingying , Shi Zhenghan , An Zijian , Dai Chaobo , Lv Jingjiang , Liu Guang , Liang Hao , Liu Jun , Lu Yanli , Zhang Fenni , Liu Qingjun
Published: May 2024

Rapid and accurate detection of respiratory virus aerosols is highlighted for virus surveillance and infection control. Here, we report a wireless immunoassay technology for fast (within 10 min), on-site (wireless and battery-free), and sensitive (limit of detection down to fg/L) detection of virus antigens in aerosols. The wireless immunoassay leverages the immuno-responsive hydrogel-modulated radio frequency resonant sensor to capture and amplify the recognition of virus antigen, and flexible readout network to transduce the immuno bindings into electrical signals. The wireless immunoassay achieves simultaneous detection of respiratory viruses such as severe acute respiratory syndrome coronavirus 2, influenza A H1N1 virus, and respiratory syncytial virus for community infection surveillance. Direct detection of unpretreated clinical samples further demonstrates high accuracy for diagnosis of respiratory virus infection. This work provides a sensitive and accurate immunoassay technology for on-site virus detection and disease diagnosis compatible with wearable integration.
Coordinated wound responses in a regenerative animal-algal holobiont
Authors: Nanes Sarfati Dania , Xue Yuan , Song Eun Sun , Byrne Ashley , Le Daniel , Darmanis Spyros , Quake Stephen R. , Burlacot Adrien , Sikes James , Wang Bo
Published: May 2024

Animal regeneration involves coordinated responses across cell types throughout the animal body. In endosymbiotic animals, whether and how symbionts react to host injury and how cellular responses are integrated across species remain unexplored. Here, we study the acoel Convolutriloba longifissura, which hosts symbiotic Tetraselmis sp. green algae and can regenerate entire bodies from tissue fragments. We show that animal injury causes a decline in the photosynthetic efficiency of the symbiotic algae, alongside two distinct, sequential waves of transcriptional responses in acoel and algal cells. The initial algal response is characterized by the upregulation of a cohort of photosynthesis-related genes, though photosynthesis is not necessary for regeneration. A conserved animal transcription factor, runt, is induced after injury and required for acoel regeneration. Knockdown of Cl-runt dampens transcriptional responses in both species and further reduces algal photosynthetic efficiency post-injury. Our results suggest that the holobiont functions as an integrated unit of biological organization by coordinating molecular networks across species through the runt-dependent animal regeneration program.
Multiscale modelling of chromatin 4D organization in SARS-CoV-2 infected cells
Authors: Chiariello Andrea M. , Abraham Alex , Bianco Simona , Esposito Andrea , Fontana Andrea , Vercellone Francesca , Conte Mattia , Nicodemi Mario
Published: May 2024

SARS-CoV-2 can re-structure chromatin organization and alter the epigenomic landscape of the host genome, but the mechanisms that produce such changes remain unclear. Here, we use polymer physics to investigate how the chromatin of the host genome is re-organized upon infection with SARS-CoV-2. We show that re-structuring of A/B compartments can be explained by a re-modulation of intra-compartment homo-typic affinities, which leads to the weakening of A-A interactions and the enhancement of A-B mixing. At the TAD level, re-arrangements are physically described by a reduction in the loop extrusion activity coupled with an alteration of chromatin phase-separation properties, resulting in more intermingling between different TADs and a spread in space of the TADs themselves. In addition, the architecture of loci relevant to the antiviral interferon response, such as DDX58 or IFIT, becomes more variable within the 3D single-molecule population of the infected model, suggesting that viral infection leads to a loss of chromatin structural specificity. Analysing the time trajectories of pairwise gene-enhancer and higher-order contacts reveals that this variability derives from increased fluctuations in the chromatin dynamics of infected cells. This suggests that SARS-CoV-2 alters gene regulation by impacting the stability of the contact network in time.
Nonuniform and pathway-specific laminar processing of spatial frequencies in the primary visual cortex of primates
Authors: Wang Tian , Dai Weifeng , Wu Yujie , Li Yang , Yang Yi , Zhang Yange , Zhou Tingting , Sun Xiaowen , Wang Gang , Li Liang , Dou Fei , Xing Dajun
Published: May 2024

The neocortex comprises six cortical layers that play a crucial role in information processing; however, it remains unclear whether laminar processing is consistent across all regions within a single cortex. In this study, we demonstrate diverse laminar response patterns in the primary visual cortex (V1) of three male macaque monkeys when exposed to visual stimuli at different spatial frequencies (SFs). These response patterns can be categorized into two groups. One group exhibit suppressed responses in the output layers for all SFs, while the other type shows amplified responses specifically at high SFs. Further analysis suggests that both magnocellular (M) and parvocellular (P) pathways contribute to the suppressive effect through feedforward mechanisms, whereas amplification is specific to local recurrent mechanisms within the parvocellular pathway. These findings highlight the non-uniform distribution of neural mechanisms involved in laminar processing and emphasize how pathway-specific amplification selectively enhances representations of high-SF information in primate V1.
Synergistic growth of nickel and platinum nanoparticles via exsolution and surface reaction
Authors: Xu Min , Jeon Yukwon , Naden Aaron , Kim Heesu , Kerherve Gwilherm , Payne David J. , Shul Yong-gun , Irvine John T. S.
Published: May 2024

Bimetallic catalysts combining precious and earth-abundant metals in well designed nanoparticle architectures can enable cost efficient and stable heterogeneous catalysis. Here, we present an interaction-driven in-situ approach to engineer finely dispersed Ni decorated Pt nanoparticles (1-6 nm) on perovskite nanofibres via reduction at high temperatures (600-800 oC). Deposition of Pt (0.5 wt%) enhances the reducibility of the perovskite support and promotes the nucleation of Ni cations via metal-support interaction, thereafter the Ni species react with Pt forming alloy nanoparticles, with the combined processes yielding smaller nanoparticles that either of the contributing processes. Tuneable uniform Pt-Ni nanoparticles are produced on the perovskite surface, yielding reactivity and stability surpassing 1 wt.% Pt/γ-Al2O3 catalysts for CO oxidation. This approach heralds the possibility of in-situ fabrication of supported bimetallic nanoparticles with engineered compositional distributions and performance.
Ultra-narrowband and rainbow-free mid-infrared thermal emitters enabled by a flat band design in distorted photonic lattices
Authors: Sun Kaili , Cai Yangjian , Huang Lujun , Han Zhanghua
Published: May 2024

Most reported thermal emitters to date employing photonic nanostructures to achieve narrow bandwidth feature the rainbow effect due to the steep dispersion of the involved high-Q resonances. In this work, we propose to realize thermal emissions with high temporal coherence but free from rainbow effect, by harnessing a novel flat band design within a large range of wavevectors. This feature is achieved by introducing geometric perturbations into a square lattice of high-index disks to double the period along one direction. As a result of the first Brillouin zone halving, the guided modes will be folded to the Γ point and interact with originally existing guided-mode resonances to form a flat band of dispersion with overall high Q. Despite the use of evaporated amorphous materials, we experimentally demonstrate a thermal emission with the linewidth of 23 nm at 5.144 μm within a wide range of output angles (from −17.5° to 17.5°).
Mapping of mitogen and metabolic sensitivity in organoids defines requirements for human hepatocyte growth
Authors: Hendriks Delilah , Artegiani Benedetta , Margaritis Thanasis , Zoutendijk Iris , Chuva de Sousa Lopes Susana , Clevers Hans
Published: May 2024

Mechanisms underlying human hepatocyte growth in development and regeneration are incompletely understood. In vitro, human fetal hepatocytes (FH) can be robustly grown as organoids, while adult primary human hepatocyte (PHH) organoids remain difficult to expand, suggesting different growth requirements between fetal and adult hepatocytes. Here, we characterize hepatocyte organoid outgrowth using temporal transcriptomic and phenotypic approaches. FHs initiate reciprocal transcriptional programs involving increased proliferation and repressed lipid metabolism upon initiation of organoid growth. We exploit these insights to design maturation conditions for FH organoids, resulting in acquisition of mature hepatocyte morphological traits and increased expression of functional markers. During PHH organoid outgrowth in the same culture condition as for FHs, the adult transcriptomes initially mimic the fetal transcriptomic signatures, but PHHs rapidly acquire disbalanced proliferation-lipid metabolism dynamics, resulting in steatosis and halted organoid growth. IL6 supplementation, as emerged from the fetal dataset, and simultaneous activation of the metabolic regulator FXR, prevents steatosis and promotes PHH proliferation, resulting in improved expansion of the derived organoids. Single-cell RNA sequencing analyses reveal preservation of their fetal and adult hepatocyte identities in the respective organoid cultures. Our findings uncover mitogen requirements and metabolic differences determining proliferation of hepatocytes changing from development to adulthood.
Evolution-guided Bayesian optimization for constrained multi-objective optimization in self-driving labs
Authors: Low Andre K. Y. , Mekki-Berrada Flore , Gupta Abhishek , Ostudin Aleksandr , Xie Jiaxun , Vissol-Gaudin Eleonore , Lim Yee-Fun , Li Qianxiao , Ong Yew Soon , Khan Saif A. , Hippalgaonkar Kedar
Published: May 2024

The development of automated high-throughput experimental platforms has enabled fast sampling of high-dimensional decision spaces. To reach target properties efficiently, these platforms are increasingly paired with intelligent experimental design. However, current optimizers show limitations in maintaining sufficient exploration/exploitation balance for problems dealing with multiple conflicting objectives and complex constraints. Here, we devise an Evolution-Guided Bayesian Optimization (EGBO) algorithm that integrates selection pressure in parallel with a q-Noisy Expected Hypervolume Improvement (qNEHVI) optimizer; this not only solves for the Pareto Front (PF) efficiently but also achieves better coverage of the PF while limiting sampling in the infeasible space. The algorithm is developed together with a custom self-driving lab for seed-mediated silver nanoparticle synthesis, targeting 3 objectives (1) optical properties, (2) fast reaction, and (3) minimal seed usage alongside complex constraints. We demonstrate that, with appropriate constraint handling, EGBO performance improves upon state-of-the-art qNEHVI. Furthermore, across various synthetic multi-objective problems, EGBO shows significative hypervolume improvement, revealing the synergy between selection pressure and the qNEHVI optimizer. We also demonstrate EGBO’s good coverage of the PF as well as comparatively better ability to propose feasible solutions. We thus propose EGBO as a general framework for efficiently solving constrained multi-objective problems in high-throughput experimentation platforms.
Representation of internal speech by single neurons in human supramarginal gyrus
Authors: Wandelt Sarah K. , Bjånes David A. , Pejsa Kelsie , Lee Brian , Liu Charles , Andersen Richard A.
Published: May 2024

Speech brain–machine interfaces (BMIs) translate brain signals into words or audio outputs, enabling communication for people having lost their speech abilities due to diseases or injury. While important advances in vocalized, attempted and mimed speech decoding have been achieved, results for internal speech decoding are sparse and have yet to achieve high functionality. Notably, it is still unclear from which brain areas internal speech can be decoded. Here two participants with tetraplegia with implanted microelectrode arrays located in the supramarginal gyrus (SMG) and primary somatosensory cortex (S1) performed internal and vocalized speech of six words and two pseudowords. In both participants, we found significant neural representation of internal and vocalized speech, at the single neuron and population level in the SMG. From recorded population activity in the SMG, the internally spoken and vocalized words were significantly decodable. In an offline analysis, we achieved average decoding accuracies of 55% and 24% for each participant, respectively (chance level 12.5%), and during an online internal speech BMI task, we averaged 79% and 23% accuracy, respectively. Evidence of shared neural representations between internal speech, word reading and vocalized speech processes was found in participant 1. SMG represented words as well as pseudowords, providing evidence for phonetic encoding. Furthermore, our decoder achieved high classification with multiple internal speech strategies (auditory imagination/visual imagination). Activity in S1 was modulated by vocalized but not internal speech in both participants, suggesting no articulator movements of the vocal tract occurred during internal speech production. This work represents a proof-of-concept for a high-performance internal speech BMI.
Quasi-static testing of UHPC cupped socket piers-footing connection and its seismic fragility analysis under near-fault ground motions
Authors: Yang Dahai , Wu Zhigang , Zuo Rui , Li Jianluan , Xie Haihui , Zhang Yingao
Published: May 2024

Assembly construction is extensively employed in bridge construction due to its ability to accelerate construction and improve quality. To speed the recovery of bridges after major earthquakes, this study proposes an assembled connection for precast piers and footings based on assembly construction. The precast piers are connected to the footings using ultra-high-performance concrete (UHPC) post-cast cupped sockets. Two specimens are tested with a 1:4 scale, namely, the cast-in-place (CIP) specimen and, the UHPC cupped socket pier specimen. Finite element models (FEM) of a continuous girder bridge with cupped socket connections are developed and verified by experimental results. The seismic fragility analysis is conducted to investigate the difference between the cupped socket connection and the CIP connection. The experimental results showed that the plastic hinge was formed on the precast piers and there was little damage to the UHPC sockets. The results of FEA indicate that UHPC cupped socket piers have slightly higher seismic fragility than the seismic fragility of cast-in-place piers. Then, some methods were proposed to reduce the seismic fragility of UHPC cupped socket piers, and their availability was confirmed by comparing them with the seismic fragility of CIP piers. Finally, an example bridge with this connection is introduced to illustrate replacing prefabricated piers after an earthquake.