bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessment of the influence of using green tea waste and fish waste as soil amendments for biosolarization on the growth of lettuce (Lactuca sativa L. var. ramosa Hort.)
Authors: Zou Yunfan , Qiu Bixia , Lin Fanqi , Wu Wanfei , Guo Runlin , Xing Jiani , Zhao Zihui , Shpigelman Avi , Achmon Yigal
Published: June 2023

Introduction

Safe and efficient treatment of organic waste is crucial to developing a sustainable food system around the world. Soil biosolarization (SBS) is a soil treatment technique that can use organic solid wastes to treat the soil in a way that is alternative to the use of chemical fumigants to improve soil fertility in agriculture.

Methods

In this study, two types of organic food wastes, green tea waste (GTW) and fish waste (FW), were evaluated for the feasibility of being applied as soil amendments within simulations of high-temperature cycle SBS. The evaluation was conducted by execution of three groups of measurements: gas and organic volatile emission profile, residual soil phytotoxicity and weed suppression, and cultivar growth (Lactuca sativa L. var. ramosa Hort.).

Results and Discussion

Green tea waste contributed to elevated levels of soil respiration and the evolution of signature volatile organic compounds during the simulated SBS. In the soil amended with green tea waste and then undergoing SBS the phyto compatibility was restored after residual phytotoxicity dissipation and a complete weed suppression was achieved. By using an application rate of 2.5% (w/w, mass fraction of green tea waste in total soil-waste mixture) green tea waste cultivar growth comparable to that of the non-treated soil (NTS) group was attained, with a more efficient nitrogen utilization and higher residual soil nitrogen content enabling the improvement of the continuous cropping system. FW at 1% (w/w, mass fraction of FW in total soil-waste mixture) promoted cultivar growth despite the significant reduction of the nitrogen (p value=0.02) and phosphorus (p value=0.03) contents in the cultivar leaves. A significant increase of the sodium content together with an increase of iron and chromium, which exceeded the permissible limit, were observed. These results provide new information about amendment selection for the SBS process.
Full-endoscopic removal of third ventricular colloid cysts: technique, results, and limitations
Authors: Unal Tugrul Cem , Sencer Altay , Dolas Ilyas , Gulsever Cafer Ikbal , Sahin Duran , Dolen Duygu , Ozata Musa Samet , Ozturk Metehan , Aras Yavuz , Aydoseli Aydin
Published: June 2023

Introduction

Colloid cysts (CCs) are rare benign lesions that usually arise from the roof of the third ventricle. They may present with obstructive hydrocephalus and cause sudden death. Treatment options include ventriculoperitoneal shunting, cyst aspiration, and cyst resection microscopically or endoscopically. This study aims to report and discuss the full-endoscopic technique for removing colloid cysts.

Materials and methods

A 25°-angled neuroendoscope with an internal working channel diameter of 3.1 mm and a length of 122 mm is used. The authors described the technique of resecting a colloid cyst by a full-endoscopic procedure and evaluated the surgical, clinical, and radiological results.

Results

Twenty-one consecutive patients underwent an operation with a transfrontal full-endoscopic approach. The swiveling technique (grasping the cyst wall and rotational movements) was used for CC resection. Of these patients, 11 were female, and ten were male (mean age, 41 years). The most frequent initial symptom was a headache. The mean cyst diameter was 13.9 mm. Thirteen patients had hydrocephalus at admission, and one needed shunting after cyst resection. Seventeen patients (81%) underwent total resection; 3 (14%), subtotal resection; and 1 (5%), partial resection. There was no mortality; one patient had permanent hemiplegia, and one had meningitis. The mean follow-up period was 14 months.

Conclusion

Even though microscopic resection of cysts has been widely used as a gold standard, successful endoscopic removal has been described recently with lower complication rates. Applying angled endoscopy with different techniques is essential for total resection. Our study is the first case series to show the outcomes of the swiveling technique with low recurrence and complication rates.
Integrated oxidative stress score for predicting prognosis in stage III gastric cancer undergoing surgery
Authors: Liu Yu-hang , Meng Rui , Zhu Bing , Zhan Qi-qi , Yang Xin , Ding Guan-yi , Jia Chun-liang , Liu Qian-yu , Xu Wei-guo
Published: June 2023

Objective: This study aimed to develop a novel scoring system, named the integrated oxidative stress score (IOSS), based on oxidative stress indices to predict the prognosis in stage III gastric cancer.

Methods: Retrospective analysis of stage III gastric cancer patients who were operated on between January 2014 and December 2016 were enrolled into this research. IOSS is a comprehensive index based on an achievable oxidative stress index, comprising albumin, blood urea nitrogen, and direct bilirubin. The patients were divided according to receiver operating characteristic curve into two groups of low IOSS (IOSS ≤ 2.00) and high IOSS (IOSS > 2.00). The grouping variable was performed by Chi-square test or Fisher’s precision probability test. The continuous variables were evaluated by t-test. The disease free survival (DFS) and overall survival (OS) were performed by Kaplan-Meier and Log-Rank tests. Univariate Cox proportional hazards regression models and stepwise multivariate Cox proportional hazards regression analysis were determined to appraise the potential prognostic factors for DFS and OS. A nomogram of the potential prognostic factors by the multivariate analysis for DFS and OS was established with R software. In order to assess the accuracy of the nomogram in forecasting prognosis, the calibration curve and decision curve analysis were produced, contrasting the observed outcomes with the predicted outcomes.

Results: The IOSS was significantly correlated with the DFS and OS, and was a potential prognostic factor in patients with stage III gastric cancer. Patients with low IOSS had longer survival (DFS: χ2 = 6.632, p = 0.010; OS: χ2 = 6.519, p = 0.011), and higher survival rates. According to the univariate and multivariate analyses, the IOSS was a potential prognostic factor. The nomograms were conducted on the potential prognostic factors to improve the correctness of survival prediction and evaluate the prognosis in stage III gastric cancer patients. The calibration curve indicated a good agreement in 1-, 3-, 5-year lifetime rates. The decision curve analysis indicated that the nomogram’s predictive clinical utility for clinical decision was better than IOSS.

Conclusion: IOSS is a nonspecific tumor predictor based on available oxidative stress index, and low IOSS is found to be a vigorous factor of better prognosis in stage III gastric cancer.
Comparing the Innovation and Export Performance of Hungarian Family and Non-Family Enterprises: Experiences Drawn from Empirical Surveys
Authors: Csákné Filep Judit , Radácsi László , Szennay Áron
Published: June 2023

Family enterprises are considered to be one of the oldest and still dominant forms of business organisations. However, their innovation activity is an emerging area of research, especially in the Central and Eastern European region. The aim of our study is to address this research gap by investigating, with the help of statistical tools, the relationship between the familiness of the firms, their innovation activity, and their export activity on representative samples of Hungarian SMEs in the years 2017, 2020, and 2022. Our results suggest that the innovation activity of exporting Hungarian SMEs is more significant than that of non-exporting SMEs. However, the relationship between familiness and innovation activity is not uniform in the three analyzed years, and the relationship between familiness and export activity is not significant. The findings of this study, that firms’ innovation and exporting activities can be mutually reinforcing, can be used by policymakers, curriculum developers in business education, and entrepreneurs. One of the main limitations of our research is that the 2020 and 2022 surveys were conducted after the first wave of the COVID-19 pandemic and the shock of the Russian–Ukrainian war, respectively; thus, the decisions of the firms could have been influenced by the unpredictability of the external environment.
Agricultural Citizen Science and Sustainable Regional Development
Authors: Merciu Florentina-Cristina , Teodorescu Camelia
Published: June 2023

In the last 70 years, agriculture has evolved from an activity that was traditionally linked to rural areas to a global industry that provides food for the growing world population [...]
Genome Identification and Characterization of WRKY Transcription Factor Gene Family in Mandarin (Citrus reticulata)
Authors: Maheen Nimra , Shafiq Muhammad , Sadiq Saleha , Farooq Muhammad , Ali Qurban , Habib Umer , Shahid Muhammad Adnan , Ali Asjad , Ali Fawad
Published: June 2023

WRKY proteins are an important group of transcription factors (TFs) gene family and were identified primarily in plants. WRKY TFs play vital roles in modulating gene expression when plants face detrimental effects due to the environment. In the current study, we focused on using the mandarin citrus (Citrus reticulata) genome to understand the impact of the WRKY gene family on the extraction of alleles mining in mandarins. The mining of the C. reticulata genome identified 46 CrWRKY genes that were classified into three main groups (G1, G2, and G3) further with five subclasses (IIa, IIb, IIc, Iid, and IIe) in the G2 group, and all were presented on 29 scaffolds representing numerous segmental duplications of 100% events established. Multiple sequence analysis predicted the presence of the “WRKYGQK” domain and metal-chelating zinc-finger motif C2H2 in 45 genes, while the “WRKYGQK” domain was replaced with “WRKYGKK” only in CrWRKY20. The comparative relationship of CrWRKY with other plant species using dual synteny analysis revealed that the divergence between C. reticulata and C. grandis occurred after the evolutionary divergence of C. clementine, C. sinensis, C. medica, and C. ichangensis. The possible functions of the CrWRKY genes in mitigating environmental effects were predicted using cis-regulatory elements analysis and in silico RNAseq analysis, for the development of plants. These results provide a robust platform and absence of knowledge for the functional identification from key genes of CrWRKY genes in the mandarin for the possible use to improve key desirable agronomic and consumer-driven fruit quality traits in mandarins and related species.
Plant-Based Beverages from Germinated and Ungerminated Seeds, as a Source of Probiotics, and Bioactive Compounds with Health Benefits—Part 1: Legumes
Authors: Criste Adriana Dalila , Urcan Adriana Cristina , Coroian Cristian Ovidiu , Copolovici Lucian , Copolovici Dana Maria , Burtescu Ramona Flavia , Oláh Neli Kinga
Published: June 2023

Consumption of plant-based milk replacers has increased in recent years due to health benefits, benefits attributed mainly to the content of phenolic compounds, fatty acids, or bioactive compounds with antioxidant activity. In this context, we proposed to obtain two types of less studied plant-based beverages, namely lupine and chickpea beverages, as well as the possibility of getting these beverages using germinated seeds and even obtaining probiotic drinks through fermentation with Lactobacillus plantarum 299v. To evaluate the quality of the obtained products, we determined their content of proteins, fatty acids, organic acids, volatile compounds, and phenolic compounds. We evaluated the antioxidant activity of the obtained herbal drinks, and a load of probiotic microorganisms present after the fermentation process. Both lupine and chickpeas are legumes with high protein content and a range of health benefits. Fermentation with L. plantarum introduces probiotic properties and enhances the nutritional profile of these beverages. Plant-based beverages inoculated with L. plantarum can offer a convenient way to incorporate probiotics into plant-based diets, providing consumers with the benefits of both plant-based nutrition and probiotic supplementation.
Changes in the Bacterial Community Composition of Cultivated Soil after Digging up Operations for Laying a Pipeline
Authors: Bonomo Maria Grazia , Scrano Laura , Mang Stefania Mirela , Scalese Barbara Emanuela , Bufo Sabino Aurelio , Modley Lee-Ann , Buongarzone Euro , Salzano Giovanni
Published: June 2023

Our study aimed to evaluate the impact of the pipeline installation on the bacterial composition in cultivated soil by metagenomic analyses performed before the excavation and in the following three years. Differential abundance analysis was obtained using DESeq2 from the GAIA pipeline to verify the bacteriological diversity in soils collected after the reference year (2013). Soil samples presented a different distribution of taxa, especially in 2014, in which a further allocation at the phylum and family levels was observed compared to the previous year (2013). The phyla Bacteroidetes and Firmicutes increased significantly, while the phylum Actinobacteria, most abundant in 2013, showed reduced abundance; moreover, Chloroflexi and Planctomycetes decreased considerably, and Verrucomicrobia was absent. The significant differences in the taxonomic composition and structure of the soil microbial community were due to critical stress conditions following the soil excavations. The bacterial communities were capable of profound physiological and genetic changes, implementing different mechanisms for survival and adaptation to an environment with changed conditions. The implication of changes in microbial diversity before and after the mechanical insult of soil has been determined.
Effect of Ivermectin on the Expression of P-Glycoprotein in Third-Stage Larvae of Haemonchus contortus Isolated from China
Authors: Luo Xiaoping , Wang Shuyi , Feng Ying , Wang Penglong , Gong Gaowa , Guo Tianlong , Feng Xingang , Yang Xiaoye , Li Junyan
Published: June 2023

Haemonchus contortus poses a severe hazard to the healthy development of the sheep industry and threatens the welfare of sheep. Ivermectin is the primary anthelmintic used for the prevention and treatment of H. contortus parasitism. However, the widespread and uncontrolled application of ivermectin has resulted in the development and spread of resistant strains of H. contortus. P-glycoprotein (P-gp) plays important roles in the pharmacology and toxicology of ivermectin, and changes in P-gp expression levels can be used to analyze the resistance of H. contortus to ivermectin. This study aimed to analyze the effects of ivermectin on P-gp expression in H. contortus L3 larvae isolated from China and to evaluate whether changes in P-gp expression levels can be used to analyze resistant H. contortus strains. In the absence of drug treatment, the ivermectin-resistant strains isolated in China showed increased expression of P-gp11 (p < 0.01) compared with sensitive strains from elsewhere, whereas the expressions of P-gp2 and P-gp9.1 were downregulated (p < 0.01). When the same strain was compared before and after drug treatment, obvious differences in expression were observed between the different strains. Ivermectin-induced P-gp expression was found to be very complex among the L3 larvae of different strains. In addition, it was confirmed that using P-gp to determine ivermectin resistance in H. contortus strains from different geographic environments can yield different results.
Phenotypic Characterization of Creole Cattle in the Andean Highlands Using Bio-Morphometric Measures and Zoometric Indices
Authors: Rojas-Espinoza Rolando , Macedo Rassiel , Suaña Alex , Delgado Alfredo , Manrique Yan P. , Rodríguez Halley , Quispe Yesenia M. , Perez-Guerra Uri H. , Pérez-Durand Manuel G. , García-Herreros Manuel
Published: June 2023

Several Creole cattle biotypes can be found in the Andean highlands, and most of them are considered as being in risk of extinction. The main aim of the present study was to perform a phenotypic characterization of the Creole cattle in the Andean highlands using bio-morphometric measures and zoometric indices. Individuals from three different biotypes (Black ‘Negro’ (n = 57), Colour-Sided ‘Callejón’ (n = 20), and Brindle ‘Atigrado’ (n = 18)) from an experimental research center located in the Peruvian highlands were enrolled in the study. In total, seventeen morphometric parameters were evaluated and ten zoometric indices were calculated in each biotype. To test the relationship between biometric traits, correlation analyses were carried out between morphometric parameters. Differences were observed regarding different morphometric variables such as head length (HL) and rump length (RL) among cattle biotypes (p ≤ 0.05). The coefficient of variation (CV; %) regarding different morphometric parameters ranged between 11.32 for neck length (NL) and 3.63 for height at withers (HaW), which indicated low–moderate variability among morphometric variables. Differences were observed in the longitudinal pelvic index (LPI) when different zoometric indices were compared among biotypes (p ≤ 0.05). The CV regarding different zoometric indices, which ranged between 10.78 for the cephalic index (CEI) and 5.05 for LPI, indicated low variability among indices. No differences were observed in any other morphometric parameter or zoometric index among cattle biotypes or genders (p > 0.05). Finally, multiple correlations were observed between morphometric variables (p ≤ 0.05). In conclusion, it was determined that Peruvian Andean Creole cattle can be considered as a dairy-related biotype with a slight tendency for beef production (dual-purpose). The great homogeneity regarding zoometric characteristics among biotypes and genders may indicate that the Andean Creole cattle have been maintained quite isolated, avoiding the genetic influence of other foreign breeds. Finally, the phenotypic characterization including bio-morphometric measurements and zoometric indices obtained from the different Creole bovine biotypes is crucial in order to begin different conservation programs to preserve cattle breeds in the Peruvian Andean highlands.
Intake, Digestibility, Ruminal Fermentation, and In Situ Disappearance of Bermudagrass Hay by Lactating Beef Cows Offered Corn or Hominy Feed as Supplements at Two Different Rates
Authors: Nieman Christine C. , Madzonga Zibani , Young-Kenworthy Ashley N. , Coffey Kenneth P.
Published: June 2023

Hominy feed (HF) has been evaluated in feedlot and dairy rations but has not been evaluated as a supplemental energy source for lactating beef cows. The objective of this study was to determine the effect of level of HF supplementation on intake, digestibility, ruminal fermentation characteristics, and in situ dry matter (DM) disappearance of bermudagrass hay. Five ruminally cannulated lactating beef cows (body weight (BW) = 596 kg, SE = 13.9) were used in an experiment with a 5 × 5 Latin square design. Treatments were a bermudagrass hay-basal diet with low HF (LH; 0.25% BW), medium HF (MH; 0.50% BW), low ground corn (LC; 0.25% BW), medium ground corn (MC; 0.50% BW) and no supplement (CON). Cows were housed individually, and supplements were offered at 0800 daily. Hay was offered to maintain 10% refusal. Periods were 16–d, with 10 d for adaptation. Ruminal fluid was sampled on d 14 of each period for measurement of pH, volatile fatty acids, and rumen ammonia-N. An in situ degradation experiment for bermudagrass hay was completed for each diet. Hay dry-matter intake (DMI % BW) was not affected (p = 0.14) by supplement, but total DMI (DMI % BW) was greater (p ≤ 0.05) in MH and MC compared to LH and CON. Digestible DMI % BW was greater (p = 0.05) in MH compared to LC, MC was intermediate, and LH and CON were lesser (p ≤ 0.01) than all other diets. Dry-matter fill, passage rate, and retention time did not differ by diet (p ≥ 0.31). A diet × time interaction was observed for ammonia-N (p = 0.0002), and propionate (p = 0.02) time effects were observed for other parameters, but no diet effects. Bermudagrass hay’s potentially degradable fraction was greater (p ≤ 0.05) in LH than MH and CON, but effective DM degradability was not different (p = 0.39) among diets. Overall, no diets reduced hay intake or disappearance compared to CON; therefore, no negative associative effects were observed from any of the supplements at the levels offered in this study.
Heat Stress: A Serious Disruptor of the Reproductive Physiology of Dairy Cows
Authors: Dovolou Eleni , Giannoulis Themistoklis , Nanas Ioannis , Amiridis Georgios S.
Published: June 2023

Global warming is a significant threat to the sustainability and profitability of the dairy sector, not only in tropical or subtropical regions but also in temperate zones where extreme summer temperatures have become a new and challenging reality. Prolonged exposure of dairy cows to high temperatures compromises animal welfare, increases morbidity, and suppresses fertility, resulting in devastating economic losses for farmers. To counteract the deleterious effects of heat stress, cattl e employ various adaptive thermoregulatory mechanisms including molecular, endocrine, physiological, and behavioral responses. These adaptations involve the immediate secretion of heat shock proteins and cortisol, followed by a complex network of disrupted secretion of metabolic and reproductive hormones such as prolactin, ghrelin, ovarian steroid, and pituitary gonadotrophins. While the strategic heat stress mitigation measures can restore milk production through modifications of the microclimate and nutritional interventions, the summer fertility records remain at low levels compared to those of the thermoneutral periods of the year. This is because sustainment of high fertility is a multifaceted process that requires appropriate energy balance, undisrupted mode of various hormones secretion to sustain the maturation and fertilizing competence of the oocyte, the normal development of the early embryo and unhampered maternal—embryo crosstalk. In this review, we summarize the major molecular and endocrine responses to elevated temperatures in dairy cows, as well as the impacts on maturing oocytes and early embryos, and discuss the consequences that heat stress brings about in dairy cattle fertility.
Influence of Genotype on Productivity and Egg Quality of Three Hen Strains Included in a Biodiversity Program
Authors: Calik Jolanta , Obrzut Joanna
Published: June 2023

The aim of the study was to evaluate the effect of genotype on the productivity and egg quality of three hen strains included in the genetic resource protection program in Poland. The study encompassed populations of laying hens, i.e., Rhode Island Red/RIR (R-11 and K-22) and Rhode Island White (A-33). The analysis over five generations included the basic production traits, i.e., the weight of the birds at 20 weeks (g), egg weights at 33 and 53 weeks, sexual maturity, the number of eggs laid up to 56 weeks of age, and hatchability parameters. In addition, the effective population size (Ne) and flock homozygosity coefficient (Fx) were calculated for each breed. Population health during the rearing and production periods was also recorded. The study also determined egg content and shell quality traits in relation to the age of the hens. The birds were kept on litter at a stocking rate of 5 hens/m2 and fed ad libitum with a standard feed mixture for hens. Based on the results, it was concluded that the evaluated hen populations (R-11, K-22, and A-33) are valuable strains, representing a reservoir of unique phenotypic and egg quality traits. It was shown that the random mating system used in the reproduction of flocks effectively protects the populations from an increase in the degree of inbreeding. An influence of hens’ origin (genotype) and age on the performance results, as well as egg quality traits, was found. Over five generations, the evaluated hen strains were characterized by high survival rates (above 98%). The study also found a large variation between the R-11 and K-22 strains and the A-33 strain in terms of the evaluated performance traits, especially in the body and egg weights, sexual maturity age, and laying performance of hens. The earliest to start laying were hens from the K-22 strains, which also showed significantly (p ≤ 0.05) higher laying performances compared to R-11 hens. The results also indicate that the quality of eggs from hens of the compared strains varied. This was particularly true for such traits as shell color; egg, shell, and yolk weight; and shell quality. It was also shown that many egg and shell quality traits change with the age of the hens. The analysis of the obtained data indicates that the adopted methods of conservative breeding for these populations have influenced the success of the conservation program.
Effects of Weaning Age and Creep Feed Type on Growth Performance and Gut Maturation in Weaned Piglets
Authors: Lyderik Kimmie Kyed , Zhang Xuwen , Larsen Christina , Kjeldsen Niels Jørgen , Pedersen Marie Louise Madelung , Hedemann Mette Skou , Williams Andrew Richard , Amdi Charlotte , Madsen Johannes Gulmann
Published: June 2023

The objective was to study the effects of weaning in week 5 (W5) vs. week 4 (W4), as well as liquid (LF) vs. dry feed (DF), on growth performance, disaccharidase activity and nutrient transporter expression after weaning. The experiment included 12,923 pigs fed LF or DF in the pre-weaning period and a subpopulation of 15 pigs from each group, W4DF, W4LF, W5DF and W5LF, which were weighed and euthanized five days after weaning. The proximal part of the small intestine was analyzed for maltase, lactase and sucrase activity and the expression of SGLT-1, GLUT-2 and PepT-1. Pigs fed LF displayed less maltase activity (2100 vs. 2729 U/mg protein, p < 0.05) but an increased expression of SGLT-1 (∆Ct: 5.22 vs. 6.21, p = 0.01). Pigs weaned in W5 were heavier than those weaned in W4 (9.35 vs. 7.11 kg BW, p ≤ 0.05), and pigs fed LF were heavier than those fed DF (8.55 vs. 7.91 kg BW, p ≤ 0.05) five days after weaning in the subpopulation. LF pigs (21.8 kg) were heavier than DF pigs (20.6 kg) (SE 0.108, p < 0.0001), and W4 pigs (21.0 kg) were lighter than W5 pigs (21.5 kg) (SE 0.108, p = 0.01) at nine weeks. LF increased weight gain in the early post-weaning period and at nine weeks, although this was apparently not explained by accelerated gut maturation.
Comparison of Three Different Diagnostic Assays for Fibroblast Growth Factor-23 (FGF-23) Measurements in Cats: A Pilot Study
Authors: Lapsina Sandra , Nagler Nicole , Müller Simon F. , Holtdirk Annette , Kottmann Tanja , Müller Elisabeth , von Luckner Jennifer , Schäfer Ingo
Published: June 2023

Fibroblast growth factor-23 (FGF-23) is a phosphaturic hormone used to monitor chronic kidney disease (CKD) in humans. The aim of this pilot study was to compare three diagnostic assays and to assess how the results correlate with parameters of renal dysfunction in cats. Four groups of 10 cats each were formed retrospectively according to creatinine, based on IRIS staging. FGF-23 was measured using two different ELISAs (MyBioSource and Kainos ELISA FGF-23 Kit) and an automated assay on the DiaSorin Liaison platform. Measurements were performed in 40 cats. Spearman’s rank correlation coefficient showed a strong correlation between the Kainos and DiaSorin assays (ρ = 0.742/p < 0.001) and a low correlation (ρ = 0.443/p = 0.005) between the Kainos and MyBioSource assays. The measurements with the Kainos assay strongly correlated with urea (ρ = 0.835/p < 0.001) and creatinine (ρ = 0.764/p < 0.001), and moderately correlated with SDMA (ρ = 0.580/p < 0.001) and phosphorus (ρ = 0.532/p < 0.001). The results of the MyBioSource and DiaSorin assays only showed a moderate correlation with urea (ρ = 0.624/0.572) and creatinine (ρ = 0.622/0.510) concentrations (p = 0.001 each). The Kainos assay showed the strongest correlation (ρ = 0.806) with the various creatinine concentrations according to the IRIS, followed by the MyBioSource (ρ = 0.663/p < 0.001) and DiaSorin assays (ρ = 0.580/p < 0.001). Overall, the Kainos assay demonstrated the best correlations with both biomarkers and various creatinine concentrations according to the IRIS. Individual assay-based reference values should be established to make a reliable interpretation of FGF-23 levels possible to diagnose or monitor feline CKD.
Factors Affecting Enteric Emission Methane and Predictive Models for Dairy Cows
Authors: Donadia Andrea Beltrani , Torres Rodrigo Nazaré Santos , Silva Henrique Melo da , Soares Suziane Rodrigues , Hoshide Aaron Kinyu , Oliveira André Soares de
Published: June 2023

Enteric methane emission is the main source of greenhouse gas contribution from dairy cattle. Therefore, it is essential to evaluate drivers and develop more accurate predictive models for such emissions. In this study, we built a large and intercontinental experimental dataset to: (1) explain the effect of enteric methane emission yield (g methane/kg diet intake) and feed conversion (kg diet intake/kg milk yield) on enteric methane emission intensity (g methane/kg milk yield); (2) develop six models for predicting enteric methane emissions (g/cow/day) using animal, diet, and dry matter intake as inputs; and to (3) compare these 6 models with 43 models from the literature. Feed conversion contributed more to enteric methane emission (EME) intensity than EME yield. Increasing the milk yield reduced EME intensity, due more to feed conversion enhancement rather than EME yield. Our models predicted methane emissions better than most external models, with the exception of only two other models which had similar adequacy. Improved productivity of dairy cows reduces emission intensity by enhancing feed conversion. Improvement in feed conversion should be prioritized for reducing methane emissions in dairy cattle systems.
Effect of Pelagic Sargassum on In Vitro Dry Matter and Organic Matter Degradation, Gas Production, and Protozoa Population
Authors: Canul-Ku Luis Alberto , Sanginés-García José Roberto , Urquizo Edgar Aguilar , Canul-Solís Jorge Rodolfo , Valdivieso-Pérez Ingrid Abril , Vargas-Bello-Pérez Einar , Molina-Botero Isabel , Arango Jacobo , Piñeiro-Vázquez Ángel Trinidad
Published: June 2023

This study determined the effect of pelagic Sargassum on in vitro dry matter and organic matter degradation, total gas production (TGP), and protozoa population. The treatments were different levels of Sargassum inclusion on a basal substrate (Stargrass hay; Cynodon nlemfuensis) as follows: T0 (control treatment based on Stargrass hay), T10 (90% Stargrass hay + 10% Sargassum), T20 (80% Stargrass hay + 20% Sargassum), and T30 (70% Stargrass hay + 30% Sargassum). Ruminal fermentation kinetics and protozoa population were determined during 72 h of in vitro incubations. Compared to control, dry matter degradability at 48 and 72 h and organic matter degradability at 24 and 48 h were higher in Sargassum treatments. TGP was lower with T20 at 48 h. The total population of protozoa and the concentration of Entodinium spp. were lower at T20 at 48 h and T30 at 72 h. Cl, S, Ca, K, and Zn (103, 5.97, 88.73, 285.70 g/kg, and 15,900 mg/kg) were high in Sargassum, reaching twice or even nine times higher than the contents in Stargrass (11.37, 1.60, 43.53, 87.73 g/kg, and 866.67 mg/kg). Overall, up to 30% pelagic Sargassum could be included in hay-based substrates from tropical grasses without negative effects on in vitro dry matter and organic matter degradability.
Performing Fatigue State Characterization in Railway Steel Bridges Using Digital Twin Models
Authors: Nhamage Idilson , Dang Ngoc-Son , Horas Claúdio , Poças Martins João , Matos José , Calçada Rui
Published: June 2023

Railway infrastructures play a pivotal role in developing the national transportation system. Recently, the strategy of the railway engineer has been significantly shifted; along with the development of new assets, they tend to pay increasing attention to the operation and management of existing railway assets. In this regard, this paper proposes a Digital Twin (DT) model to improve fatigue assessment efficiency in the operational processes of railway steel bridges (RSBs). The DT concept mainly lies in the federation and interaction of a Fatigue Analysis System (FAS), which is based on Eurocodes principles, and a model in Building Information Modeling (BIM). Along with the proposed DT concept, a prototyping system for a real bridge is initiated and curated. The FAS is validated in good-agreement results with the ambient vibration test of the bridge (about 1.6% variation between numerical and experimental values), and close values were found between numerical and experimental stresses, the latter obtained by installing strain gauges on the bridge. The BIM model provides access to the numerical values of fatigue state results in a given bridge connection detail but also automatically represents that information in a 3D environment using a color-scale-based visualization process. Furthermore, a simulation model with the main input variables being the traffic and geometric conditions of the bridge is continuously updated for timely re-evaluation of the damage state, which shows promise for the lifecycle management of the bridge.
Double-Bend and Semi-Spherical Energy Deflectors’ Blast Load Efficiency—A Comparative Study
Authors: Konarzewski Marcin , Panowicz Robert , Jeschke Adam
Published: June 2023

The experience of recent military missions clearly shows the importance of protecting vehicles and the people they carry from mines and IEDs. This protection can be achieved in many ways, using both active and passive solutions. One of the most popular solutions is using energy deflectors, which, thanks to their appropriately selected shape, can dissipate the energy from the detonation of an explosive charge, thus reducing the blast wave loading on the protected structure. The article presents the research results on two innovative groups of deflectors—double-bend and semi-spherical, characterized by a better ability to disperse energy than traditionally used V-shaped deflectors. Several possible geometric shapes differing in critical dimensions were selected, and both experimental studies and numerical analyses were carried out. A ballistic pendulum was used to measure individual deflectors’ performances. The tests proved the tested solutions’ increased effectiveness compared with V-shaped deflectors.
A Lightweight Model for 3D Point Cloud Object Detection
Authors: Li Ziyi , Li Yang , Wang Yanping , Xie Guangda , Qu Hongquan , Lyu Zhuoyang
Published: June 2023

With the rapid development of deep learning, more and more complex models are applied to 3D point cloud object detection to improve accuracy. In general, the more complex the model, the better the performance and the greater the computational resource consumption it has. However, complex models are incompatible for deployment on edge devices with restricted memory, so accurate and efficient 3D point cloud object detection processing is necessary. Recently, a lightweight model design has been proposed as one type of effective model compression that aims to design more efficient network computing methods. In this paper, a lightweight 3D point cloud object detection network architecture is proposed. The core innovation of the proposal consists of a lightweight 3D sparse convolution layer module (LW-Sconv module) and knowledge distillation loss. Firstly, in the LW-Sconv module, factorized convolution and group convolution are applied to the standard 3D sparse convolution layer. As the basic component of the lightweight 3D point cloud object detection network proposed in this paper, the LW-Sconv module greatly reduces the complexity of the network. Then, the knowledge distillation loss is used to guide the training of the lightweight network proposed in this paper to further improve the detection accuracy. Finally, extensive experiments are performed to verify the algorithm proposed in this paper. Compared with the baseline model, the proposed model can reduce the FLOPs and parameters by 3.7 times and 7.9 times, respectively. The lightweight model trained with knowledge distillation loss achieves comparable accuracy to the baseline. Experiments show that the proposed method greatly reduces the model complexity while ensuring detection accuracy.
Multiple Fano Resonances in Dynamic Resonant Tunneling Processes
Authors: Zangwill Gilad , Granot Er’el
Published: June 2023

The existence of Fano resonances in dynamic resonant tunneling (RT) systems has been investigated. Fano resonances are characterized by the appearance of a 100% reflection coefficient in proximity to a high transmission coefficient. For a Fano resonance to appear, a bound state must exist. On the other hand, a resonant tunneling process is characterized by a high transmission and the existence of a quasi-bound state (QBS) instead of a bound one. It has been shown that, by narrowing the width of the barrier, the resonance energy of the QBS gradually decreases and eventually turns into a bound state. Consequently, in a dynamic RT process, there are two scenarios: either a bound state exists, in which case, Fano resonances exist for any barrier width, or a QBS exists, and the barrier should be narrow enough for the Fano resonance to appear. In both cases, the incoming particle’s frequency must be lower than the oscillating well’s frequency. In this work, these resonances are investigated in detail, and both exactly numerically and approximated analytical expressions are derived for both the weak and strong oscillating amplitude regimes. One of the conclusions is that, when the oscillating frequency is low enough, multiple Fano resonances can appear by varying the barrier’s width. Since these resonances are very sharp and zero transmission can easily be detected, this property can be used as a very accurate method for measuring the barrier’s width, even when the particle’s de-Broglie wavelength is much larger than the barrier’s width.
Correction of Temperature from Infrared Cameras for More Precise As-Is 3D Thermal Models of Buildings
Authors: Adán Antonio , Pérez Víctor , Ramón Amanda , Castilla Francisco J.
Published: June 2023

This paper studies how to create precise 3D thermal maps of building interiors by correcting the raw apparent temperature values yielded by the thermal cameras. This matter has not, to date, been dealt with in the literature, which leads us to conclude that the current methodologies that obtain thermal point clouds are incomplete and imprecise. Without a suitable correction, any further energy parameter calculation obtained from the thermal point cloud is incorrect. This paper presents a method that deals with important aspects to be corrected, such as the repeatability of thermal cameras, the use of the true emissivity of the materials sensed, and the inclusion of the reflected radiant energy caused by the environment. The method has been successfully tested in several indoor scenes using a thermal scanning platform. The results show that significant corrections of up to 8% of the raw temperature values must be carried out in the final thermal model, thus justifying the need for the correction. As an application of the method, an empirical calculation and a comparison of transmittances with and without temperature corrections are presented at the end of the paper. In this case, the relative errors with respect to the average nominal U-value decrease from 94% to 11%. The general conclusion is that precise calculations of energy parameters in which infrared cameras are involved must take these corrections to temperature into account.
SoundFields: A Virtual Reality Home-Based Intervention for Auditory Hypersensitivity Experienced by Autistic Children
Authors: Parkinson Sarah , Schumann Sophie , Taylor Amelia , Fenton Clare , Kearney Gavin , Garside Megan , Johnston Daniel
Published: June 2023

Previous studies have shown that autistic people often display atypical responses when processing sensory information, with particular prevalence within the auditory domain. Often provoked by common everyday sounds, auditory hypersensitivity can result in self-regulatory fear responses. This can be potentially harmful to autistic individuals and the people around them and is associated with greater occurrence of anxiety, depression, and poorer overall quality of life in the autistic population. Rather than a physiological causation, the literature suggests that hypersensitivity to sound is likely to be caused by how auditory stimuli are processed in the brain. This paper reports a home-based digital intervention aimed to address auditory hypersensitivity in autistic children. Developed as an interactive virtual reality game, the system integrates exposure-based therapy techniques into game mechanics and delivers target auditory stimuli to the player rendered via binaural-based spatial audio. The performance of the platform was evaluated in a 10-week feasibility study, during which children (n = 7) engaged weekly with the game during a 30 min session. Following this period, a comparison of pre- and post-study measurements showed a decrease in sensitivity for five participants, with qualitative feedback highlighting an increase in tolerance towards real-world stimuli and challenging environments. These results provide initial support for SoundFields as a home-based intervention targeting auditory hypersensitivity experienced by autistic children.
Research on the Technologies of the Compressor Remanufacturing Process
Authors: Pan Qiaosheng , Wan Aode , Zhang Chi , Wei Zeping
Published: June 2023

This study describes the remanufacturing process scheme and key technologies of waste compressors. Firstly, the technical characteristics of compressors for home appliances such as refrigerators and air conditioners are summarised. Then, the remanufacturing process scheme is proposed with the waste refrigerator compressor as the research object. The purpose or requirements of key processes such as separation, disassembling, cleaning, detecting, remanufacturing and reassembling are also elaborated. Moreover, the corresponding remanufacturing technology or method is proposed to satisfy the structural, quality requirement, economy and environmental friendliness of products and components. The cleaning technology of the compressor motor stator and the remanufacturing technology of crankshaft is highlighted. In accordance with the structural and quality requirement of the motor stator, the cleaning method of the motor stator is proposed, and the environmental friendliness, economy and safety of several cleaning agents are analysed. The scheme of crankshaft remanufacturing is proposed on the basis of the failure form of the crankshaft and the quality requirement of remanufacturing. Moreover, the process parameters are described.
Probiotic Lactic Acid Bacteria for Vaginal Application. Optimization of Biomass Production and Freeze-Drying Conditions
Authors: Marchesi Antonella , Nader-Macías María Elena Fátima
Published: June 2023

Probiotic formula for the vaginal tract must contain high numbers of viable beneficial bacteria that maintain their characteristics during the production and further storage, in order to exert the claimed probiotic effect. Four probiotic strains—Lactobacillus gasseri CRL1320, Limosilactobacillus reuteri CRL1324, Ligilactobacillus salivarius CRL1328 and Lacticaseibacillus rhamnosus CRL1332—originally isolated from vagina of healthy women exhibiting beneficial properties were evaluated. Therefore, the optimization of growth parameters and dry biomass production with high viability while preserving probiotic features of strains is a great challenge. Different growth conditions in MRS medium were set up in a laboratory bioreactor; two initial pH and agitation were recorded speeds during static or controlled fermentations. Production conditions of 37 °C with controlled pH 6.5 and 5.5-MRS with 150 and 75 rpm stirring speeds were used for CRL1329 and CRL1332 strains, respectively, while static and free pH MRS for CRL1324 and CRL1328 probiotics allowed us to obtain maximal cell viability counts. However, during 12 h of fermentation, biomass yields of 19.3, 16.2 and 15.2 g/L were achieved when CRL1329, CRL1328 and CRL1324 probiotic strains were grown in static and free pH MRS. The highest biomass yield for CRL1332 strain was produced under controlled MRS-initial pH 6.5 and 75 rpm fermentation conditions. To preserve probiotic high biomass viability, freeze-drying was carried out in the presence of different cryoprotective agents. Thus, the highest viable numbers (10.9–11.8 log CFU/g) with survival rates between 91.3 and 95.6% were attained in the presence of 10% trehalose (L. reuteri and L. salivarius), lactose (L. rhammosus) and lactose + trehalose + sucrose mix (L. gasseri). When stability during post-freeze-drying storage was evaluated, probiotic strains showed a remarkably higher viability recovery when stored at 4 °C than at 25 °C for 12 and 3 months, respectively. In addition, surface characteristics of vaginal probiotics were affected to different extents during storage depending on the strain, protective agent and storage time/temperature. Critical factors for growth conditions, drying process and storage stability of probiotic lactobacilli strains were optimized in view to preserve cell high viability and surface features for the design of vaginal probiotic formula.
Hearing Outcomes Following Endolymphatic Duct Blockage Surgery and Factors Associated with Improved Audition at Two Years Follow-Up
Authors: Saliba Issam , Asmar Marc-Henri
Published: June 2023

Objective: To evaluate hearing outcomes at 2 years post endolymphatic duct blockage (EDB) surgery, with an analysis of factors that may predict hearing improvement. Study Design: Retrospective comparative study. Setting: Tertiary care center. Subjects: Definite Ménière’s Disease (MD) patients undergoing EDB for refractory disease. Methods: Chart review was conducted to assign cases to one of the three hearing outcome groups (deteriorated, stable, and improved). All cases that met our inclusion criteria were selected. Preoperative data collected were audiograms, bithermal caloric tests, preoperative vertigo episodes, history of previous ear surgery for Ménière, intratympanic steroid injections (ITS) and intraoperative endolymphatic sac (ELS) tear or opening. Postoperative data collected at 24 months were audiograms, vertigo episodes and bithermal caloric testing. Results: Preoperative vertigo episodes, caloric paresis and history of surgery, ITS injections or ELS integrity, as well as postoperative vertigo class distribution and caloric paresis changes were not different between our groups. Preoperative word recognition score (WRS) was lowest in the improved hearing group (p = 0.032). The persistence of tinnitus at 2 years postoperatively was associated with deteriorated hearing (p = 0.033). Conclusions: There are no strong predictors of hearing improvement on presentation pre-EDB, but low preoperative WRS may be the best estimator available. Therefore, ablative interventions should be considered very carefully in patients presenting with low WRS, as they may benefit more from EDB; there is a fair chance of a good hearing outcome with EDB surgery. Persistence of tinnitus can reflect deteriorating audition. Vertigo control and hearing preservation are independent outcomes of EDB surgery, making it desirable as an early intervention for refractory MD cases.
Editorial Conclusion for the Special Issue “Special Issue in Honor of the 60th Birthday of Professor Hong-Kun Xu”
Authors: Du Wei-Shih , Muglia Luigi , Petruşel Adrian
Published: June 2023

In the original article [...]