bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of Alfalfa (Medicago sativa) and Sericea Lespedeza (Lespedeza cuneata) to Improve Animal Performance in a Tall Fescue-Based Grazing System
Authors: Tracy Benjamin , Fike John , Brennan Kelsey , Blackmon Tianna , Kaur Sukhmanpreet
Published: April 2022

Tall fescue [Schedonorus arundinaceous (Schreb.) Dumort. nom. cons. Lolium arundinaceum (Schreb.) Darbysh.] is the dominant pasture grass across much of the southeast of the USA. The species is productive, but grows slowly in summer. Plants also harbor an endophytic fungus (Epichloe coenophiala), which produces alkaloids that are toxic to cattle. Adding summer-productive forages to fescue-based systems may benefit animal production by providing extra herbage and diluting fescue toxins. A three-year study was conducted in Virginia, USA to determine animal and vegetation responses when alfalfa (Medicago sativa) or Sericea lespedeza (Lespedeza cuneata (Dum. Cours.) G. Don) swards were established into tall fescue pastures. Average daily gain (ADG) of steers and seasonal herbage mass dynamics were monitored from 2016 to 2018. Forage and weed species composition measurements were collected to address a secondary objective that sericea might suppress weeds through allelopathy. Steer performance was acceptable (0.73 kg d−1 ADG), but interseeded legumes did not improve weight gain. Steers avoided sericea plants, and this resulted in greater herbage mass accumulation in summer compared with other treatments. Alfalfa was selectively grazed and cover decreased to almost zero by year 3, while sericea cover increased to over 82%. We found little evidence that sericea was allelopathic against weeds. Neither summer-productive legume species proved to be satisfactory in improving summer animal performance in this tall fescue-based grazing system.
Digestate and Manure Use in Kohlrabi Production: Impact on Plant-Available Nutrients and Heavy Metals in Soil, Yield, and Mineral Composition
Authors: Kovačević Dragan , Manojlović Maja , Čabilovski Ranko , Ilić Zoran S. , Petković Klara , Štrbac Mirna , Vijuk Mirjana
Published: April 2022

Digestate is a residue of the anaerobic decomposition of organic waste for biogas extraction, but it can be reused as a source of nutrients. To examine the effect of digestate in kohlrabi production, field experiments were conducted during three seasons in two calendar years. The fertilization treatments included the application of solid digestate (two rates—DS1 and DS2), liquid digestate (two rates—DL1 and DL2), solid manure (two rates—MS1 and MS2), and mineral fertilizer (NPK) and were compared with a plot without fertilization (Ø). The results showed a significant increase in the yield with the use of solid and liquid digestate, as well as with NPK, in all growing seasons, while the microelement contents (Zn, Mn, and Cu) in the leaves were at optimum level. The applied treatments did not increase the plant-available nutrients (AL-P2O5, AL-K2O, Fe, Cu, and Zn) in the soil (except Mn). The application of DL2, MS1, and MS2 led to a higher Pb content in kohlrabi stems compared to the control, but the Pb content remained below the maximum permitted limit. Our research showed that digestate can be used as a valuable source of nutrients for kohlrabi production, with a low risk of soil and plant contamination by heavy metals. However, the control of soil, digestates, and manure quality is recommended.
Yam (Dioscorea rotundata Poir.) Displays Prezygotic and Postzygotic Barriers to Prevent Autogamy in Monoecious Cultivars
Authors: Denadi Narcisse , Yolou Mounirou , Dadonougbo Ayènan Eric , Zoundjihékpon Jeanne , Dansi Alexandre , Gandonou Christophe , Quinet Muriel
Published: April 2022

Cultivated yam (D. rotundata) is a staple tuber crop in West Africa whose sexual reproduction control remains largely unknown despite its importance for plant breeding programs. In this paper, we compared self-pollination, intracultivar cross-pollination and intercultivar cross-pollination in three monoecious cultivars (Amoula, Heapala and Yassi). Results showed that pollen viability (49%) and stigma receptivity (40%) were similar in monoecious and dioecious plants, suggesting that autogamy could occur in monoecious plants. However, fruit and seed sets were significantly lower after self-pollination compared to cross-pollination. Overall, autogamy reached 11% and pollen lability was almost zero (<1%). The low percentage of pollen grains germinating on the stigma (37%) and pollen tubes reaching the ovules (25%) after self-pollination partly explained the low seed set. Strong inbreeding depression was observed after self-pollination and almost all fruits and about 75% of the seeds resulting from self-pollination showed malformations. Seed germination was also 20 times lower after self-pollination compared to cross-pollination. Sexual reproduction remained low in D. rotundata even after cross-pollination as fruit and seed set did not exceed 18% and 13% respectively. Moreover, comparison between intracultivar cross-pollination and self-pollination revealed intravarietal genetic diversity inside the analyzed yam cultivars. Overall, our results showed that D. rotundata has a very low tolerance to autogamy in monoecious cultivars and has developed pre- and postzygotic mechanisms to limit selfing.
Correction: Le Dinh et al. Effect of Diet Composition on Excreta Composition and Ammonia Emissions from Growing-Finishing Pigs. Animals 2022, 12, 229
Authors: Le Dinh Phung , van der Peet-Schwering Carola M. C. , Ogink Nico W. M. , Aarnink André J. A.
Published: April 2022

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Effects of Mother’s Dominance Hierarchy on the Development of Social Relationships among Immature Tibetan Macaques
Authors: Liu Chuan-Chang , Chen Shi-Wang , Wei Qi-Bing , Sun Bing-Hua , Wang Xi , Xia Dong-Po
Published: April 2022

During a relatively long period of growth, immature individuals rely on their mothers to obtain nutrition, and a good environment for learning social skills needed to cope with complex environments in adulthood. In this study, we collected the behavioral data of Tibetan macaques (Macaca thibetana) to investigate the effects of females’ social rank on the development of social relationships among their immature offspring from November to June 2021. The results show that there was no difference in the rate/type of social play and grooming among infants. However, among juveniles and adolescents, the higher their mother’s social rank, the higher the rate of social play they participated in, and the more aggressive play they engaged in. Immatures with high-ranking mothers initiated more social play among each other. A similar pattern of playmates was found among juveniles/adolescents with middle-ranking and low-ranking mothers. We also found that immatures preferred immatures with higher-ranking mothers as grooming mates and initiated more grooming with immatures with higher-ranking mothers than with those with lower-ranking mothers. Our study suggests that females’ social ranks affect the development of social relationships among their immature offspring. In despotic nonhuman primates, this indicates that the mother’s dominance hierarchy would directly or indirectly influence the processes of participating in social interactions and choosing partnerships among immature individuals with age (i.e., infancy, juvenile, and adolescent periods).
Prevalence and Antimicrobial Susceptibility of Campylobacter Species with Particular Focus on the Growth Promoting, Immunostimulant and Anti-Campylobacter jejuni Activities of Eugenol and Trans-Cinnamaldehyde Mixture in Broiler Chickens
Authors: Aljazzar Ahmed , Abd El-Hamid Marwa I. , El-Malt Rania M. S. , El-Gharreb Waleed Rizk , Abdel-Raheem Sherief M. , Ibrahim Abdelazim M. , Abdelaziz Adel M. , Ibrahim Doaa
Published: April 2022

Campylobacter species (spp.) are one of the most important causes of human bacterial gastroenteritis in foods of animal origin. Recently, with the spread of multi-drug-resistant (MDR) and extensively drug-resistant (XDR) Campylobacter spp., natural alternative therapeutic methods are urgently required. Phytogenic active principles have gained considerable attention due to their proficiency to enhance gut health and, thereby, performance of broiler chickens. Thus, the current study aims to determine the prevalence and antimicrobial resistance of Campylobacter spp. of different chicken sources in Sharkia Governorate, Egypt, and to assess the growth-promoting, immunostimulant and antimicrobial effects of a mixture of eugenol and trans-cinnamaldehyde in an in vivo approach. A total of 101 (67.3%) campylobacter isolates was identified, according to both phenotypic and genotypic techniques. Moreover, all of the campylobacter isolates were resistant to erythromycin, trimethoprim/sulfamethoxazole, and ampicillin (100% each). Of note, a dietary supplementation of the mixture of eugenol and trans-cinnamaldehyde led to a significant improvement of the feed conversion ratio and body weight gain and a decrease in the cecal C. jejuni loads in the broilers challenged with XDR C. jejuni. Additionally, eugenol and the trans-cinnamaldehyde mixture had protective activities via the down-regulation of XDR C. jejuni (flaA, virB11 and wlaN) virulence genes and proinflammatory cytokines (TNF-α, IL-2, IL-6, and IL-8), and the up-regulation of anti-inflammatory cytokine IL-10. Thus, we recommend the usage of a mixture of eugenol and trans-cinnamaldehyde as an alternative to antimicrobials for the control and treatment of campylobacter infections.
Surveys on Exposure to Reptile-Associated Salmonellosis (RAS) in the Piedmont Region—Italy
Authors: Meletiadis Arianna , Biolatti Cristina , Mugetti Davide , Zaccaria Teresa , Cipriani Raffaella , Pitti Monica , Decastelli Lucia , Cimino Francesca , Dondo Alessandro , Maurella Cristiana , Bozzetta Elena , Acutis Pier Luigi
Published: April 2022

Reptile-associated salmonellosis (RAS), Salmonella infection in humans, is acquired through contact with reptiles. Reptiles have become popular pet animals, and RAS is likely to be an underestimated but growing problem. No epidemiological data about RAS are routinely collected in Italy. In order to estimate the occurrence of RAS in the Italian human population and to investigate the exposure, two epidemiological studies on patients with sporadic salmonellosis were carried out in the Piedmont region, along with an evaluation of human exposure in public places displaying reptiles and with a survey on people awareness. RAS appeared make up 7% of sporadic salmonellosis in the first study and 3% in the second, more extensive study. A prevalence of 11.7% and 5.7%, respectively, were calculated for the age range of 0–21 years. It was observed that in public places displaying reptiles, it was possible to easily come into contact with the animals and their environment. Some knowledge about RAS emerged from the interviews with the general population, but preventive measures are not completely applied by reptile owners. In conclusion, RAS in Italy is present and constitutes a proportion of the human salmonellosis cases in line with the percentages reported in other countries. Exposure to reptiles should always be considered as a risk factor, and people should be more informed about RAS and the related preventive measures.
Effects of Water Extract from Artemisia argyi Leaves on LPS-Induced Mastitis in Mice
Authors: Ma Qianbo , Wei Yuanhao , Meng Zitong , Chen Yuhua , Zhao Guoqi
Published: April 2022

In the context of the unsatisfactory therapeutic effect of antibiotics, the natural products of plants have become a research hotspot. Artemisia argyi (A. argyi) is known as a traditional medicine in China, and its extracts have been reported to have a variety of active functions, including anti-inflammatory. Therefore, after establishing the mouse mastitis model by lipopolysaccharide (LPS), the effects of A. argyi leaves extract (ALE) were evaluated by pathological morphology of the mammary gland tissue, gene expression, and serum oxidation index. Studies have shown that ALE has a restorative effect on LPS-induced mammary gland lesions and significantly down-regulated the rise of myeloperoxidase (MPO) induced by LPS stimulation. In addition, ALE played a positive role in LPS-induced oxidative imbalance by restoring the activities of glutathione peroxidase (GSH-PX) and superoxide dismutase (SOD) and preventing the increase in nitric oxide (NO) concentration caused by the over-activation of total nitric oxide synthase (T-NOS). Further analysis of gene expression in the mammary gland showed that ALE significantly down-regulated LPS-induced up-regulation of inflammatory factors IL6, TNFα, and IL1β. ALE also regulated the expression of MyD88, a key gene for toll-like receptors (TLRs) signaling, which, in turn, regulated TLR2 and TLR4. The effect of ALE on iNOS expression was similar to the effect of T-NOS activity and NO content, which also played a positive role. The IκB gene is closely related to the NF-κB signaling pathway, and ALE was found to significantly alleviate the LPS-induced increase in IκB. All of these results indicated that ALE may be considered a potential active substance for mastitis.
The Effect of Sustainable Feeding Systems, Combining Total Mixed Rations and Pasture, on Milk Fatty Acid Composition and Antioxidant Capacity in Jersey Dairy Cows
Authors: Șanta Anita , Mierlita Daniel , Dărăban Stelian , Socol Claudia Terezia , Vicas Simona Ioana , Șuteu Mihai , Maerescu Cristina Maria , Stanciu Alina Stefania , Pop Ioan Mircea
Published: April 2022

This study was carried out to assess the effect of using pasture combined with total mixed ration (TMR) on milk production and composition, fatty acid (FA) profiles, fat-soluble antioxidant content, and total milk antioxidant capacity (TAC). In addition, the effect of milk pasteurization and storage at 2 °C for 4 days, lipophilic antioxidants and TAC were assessed. Two homogeneous groups of Jersey cows (n = 8) were constituted, which were randomly assigned to one of the two treatments: TMR (without access to pasture), or partial mixed diet (pTMR: grazing 8 h/day + TMR indoors). To establish FA profiles and lipophilic antioxidants’ changes in milk during the grazing period, in case of the pTMR group the experimental period was spilt in three grazing periods: P1 (May), P2 (June), and P3 (June/July). Milk yielded from cows having limited access on pasture (pTMR diet) showed an improved FA profile, with higher concentrations of FAs considered benefic for human health (vaccenic acid (VA), conjugated linoleic acid (CLA), omega-3 FA (n-3 FA)) (p < 0.01) and a lower concentration of FAs with hypercholesterolemiant potential (C12:0, C14:0, and C16:0) (p < 0.05), compared to that of the TMR diet. This change in FA profile was correlated with improved sanogenous lipid indices of milk fat (n-6/n-3 FA atherogenic index and thrombogenic index). Milk yielded during the P1 grazing period had higher concentrations of VA, CLA, and n-3 FA (p < 0.05) and lower concentrations of C14:0 and C16:0 (p < 0.01); it exhibited the best values for the main sanogenous fat lipid indices of fat. Moreover, pTMR milk showed a higher α-tocopherol, retinol, and β-carotene content (p < 0.05), positively correlated with TAC values in milk (P1 ˃ P2 ˃ P3). By comparison, cows fed using the TMR diet yielded a higher quantity of milk (p < 0.05), but a lower fat and protein content (p < 0.01), and also a higher saturated FAs and n-6 FA content (p < 0.05) together with a lower concentration of lipophilic antioxidants in milk. Thermal treatment showed no effect on α-tocopherol and retinol content in milk, but their concentrations decreased during the storage, at the same time a TAC decrease. The results of this study prove the positive effect of using pasture combined with TMR on FA profiles, milk antioxidant content, and antioxidant capacity, with beneficial effects on nutrition and health in humans.
Cellular, Antibody and Cytokine Pathways in Children with Acute SARS-CoV-2 Infection and MIS-C—Can We Match the Puzzle?
Authors: Lazova Snezhina , Dimitrova Yulia , Hristova Diana , Tzotcheva Iren , Velikova Tsvetelina
Published: April 2022

The newly identified strain of the Coronaviridae family called severe acute respiratory syndrome (SARS-CoV-2) recently became the most significant health threat for adults and children. Some main predictors of severe clinical course in patients with SARS-CoV-2 infection are age and concomitant health conditions. Therefore, the proper evaluation of SARS-CoV-2-specific immunity is urgently required to understand and predict the spectrum of possible clinical phenotypes and recommend vaccination options and regimens in children. Furthermore, it is critical to characterize the nature of SARS-CoV-2-specific immune responses in children following asymptomatic infection and COVID-19 and other related conditions such as multisystem inflammatory syndrome (MIS-C), para-infectious and late postinfectious consequences. Recent studies involving children revealed a variety of cytokines, T cells and antibody responses in the pathogenesis of the disease. Moreover, different clinical scenarios in children were observed-asymptomatic seroprevalence, acute SARS-CoV-2 infection, and rarely severe COVID-19 with typical cytokine storm, MIS-C, long COVID-19, etc. Therefore, to gain a better clinical view, adequate diagnostic criteria and treatment algorithms, it is essential to create a realistic picture of the immunological puzzle of SARS-CoV-2 infection in different age groups. Finally, it was demonstrated that children may exert a potent and prolonged adaptive anti-SARS-CoV-2 immune response, with significant cross-reactions against other human Corona Viruses, that might contribute to disease sparing effect in this age range. However, the immunopathology of the virus has to be elucidated first.
Aging-Related Decline of Autophagy in Patients with Atrial Fibrillation—A Post Hoc Analysis of the ATHERO-AF Study
Authors: Versaci Francesco , Valenti Valentina , Forte Maurizio , Cammisotto Vittoria , Nocella Cristina , Bartimoccia Simona , Schirone Leonardo , Schiavon Sonia , Vecchio Daniele , D’Ambrosio Luca , Spinosa Giulia , D’Amico Alessandra , Chimenti Isotta , Violi Francesco , Frati Giacomo , Pignatelli Pasquale , Sciarretta Sebastiano , Pastori Daniele , Carnevale Roberto
Published: April 2022

Background: Aging is an independent risk factor for cardiovascular diseases. The autophagy process may play a role in delaying aging and improving cardiovascular function in aging. Data regarding autophagy in atrial fibrillation (AF) patients are lacking. Methods: A post hoc analysis of the prospective ATHERO-AF cohort study, including 150 AF patients and 150 sex- and age-matched control subjects (CS), was performed. For the analysis, the population was divided into three age groups: <50–60, 61–70, and >70 years. Oxidative stress (Nox2 activity and hydrogen peroxide, H2O2), platelet activation (PA) by sP-selectin and CD40L, endothelial dysfunction (nitric oxide, NO), and autophagy parameters (P62 and ATG5 levels) were assessed. Results: Nox2 activity and H2O2 production were higher in the AF patients than in the CS; conversely, antioxidant capacity was decreased in the AF patients compared to the CS, as was NO production. Moreover, sP-selectin and CD40L were higher in the AF patients than in the CS. The autophagy process was also significantly impaired in the AF patients. We found a significant difference in oxidative stress, PA, NO production, and autophagy across the age groups. Autophagy markers correlated with oxidative stress, PA, and endothelial dysfunction in both groups. Conclusions: This study provides evidence that the autophagy process may represent a mechanism for increased cardiovascular risk in the AF population.
Computational Characterization of Turbulent Flow in a Microfluidic Actuator
Authors: Laín Santiago , Lozano-Parada Jaime H. , Guzmán Javier
Published: April 2022

In this contribution, an unsteady numerical simulation of the flow in a microfluidic oscillator has been performed. The transient turbulent flow inside the device is described by the Unsteady Reynolds Averaged Navier–Stokes equations (URANS) coupled with proper turbulence models. The main characteristics of the complex fluid flow inside the device along one oscillation cycle was analyzed in detail, including not only velocity contours but also the pressure and turbulent kinetic energy fields. As a result, two-dimensional simulations provided good estimations of the operating frequency of the fluidic actuator when compared with experimental measurements in a range of Reynolds numbers. Moreover, with the objective of altering the operating frequency of the apparatus and, in order to adapt it to different applications, geometrical modifications of the feedback channels were proposed and evaluated. Finally, a fully three-dimensional simulation was carried out, which allowed for the identification of intricate coherent structures revealing the complexity of the turbulent flow dynamics inside the fluidic oscillator.
Selective Chlorination and Extraction of Valuable Metals from Iron Precipitation Residues
Authors: Höber Lukas , Witt Kerrin , Steinlechner Stefan
Published: April 2022

Due to the aggravating situations regarding climate change, resource supply, and land consumption by the landfilling of residual materials, it is necessary to develop recycling processes that allow the recovery of valuable metals from industrial residues with significantly reduced CO2 emissions. In this context, it is conceivable that processes using chlorination reactions will be of importance in the future. The simultaneous selective chlorination and evaporation of nine valuable metals was evaluated theoretically and experimentally in small-scale STA trials; then, it was tested practically on six different iron precipitation residues from the zinc and nickel industries. The metal chlorides FeCl3∙6H2O and MgCl2∙6H2O were identified as the most effective reactants, resulting in high extraction rates for the metals In, Ag, Zn, Pb, Au, and Bi, while lower yields are achievable for Sn, Cu, and Ni. Iron, which is predominant in volume in the residual materials, shows lower chlorination tendencies which allows the effective separation of the valuable elements of interest from the iron containing matrix.
A Comparative Study of a Fully-Connected Artificial Neural Network and a Convolutional Neural Network in Predicting Bridge Maintenance Costs
Authors: Wang Chongjiao , Yao Changrong , Zhao Siguang , Zhao Shida , Li Yadong
Published: April 2022

The cost assessment of bridge maintenance is a difficult topic to study, but it is critical for a bridge life cycle cost analysis. The maintenance costs sample database was established in this study according to actual engineering data, and a bridge maintenance cost prediction model was developed using a fully-connected artificial neural network (ANN) and convolutional neural network (CNN), respectively. First, eight main factors affecting maintenance costs were evaluated based on the random forest method, and the evaluation results were verified by an exploratory data analysis. The original data were then screened based on the isolation forest principle, and the recent gross domestic product (GDP) growth rate was used to illustrate the relationship between economic development and bridge maintenance costs. Finally, these two neural networks were used to establish maintenance cost prediction models, respectively. The results from the two models were compared and their prediction accuracies were analyzed. The prediction performance of the CNN model for bridge maintenance costs was found to be better than that of the traditional fully-connected ANN model. The results of this study will enhance the opportunity for bridge managers to balance lifecycle maintenance costs.
Assessment of the Resource Potential of Baltic Sea Macroalgae
Authors: Kulikova Yuliya , Sukhikh Stanislav , Kalashnikova Olga , Chupakhin Evgeny , Ivanova Svetlana , Chubarenko Boris , Gorbunova Julia , Babich Olga
Published: April 2022

The excess biomass of drifting algae and their casting to the Baltic Sea coast imposes a significant environmental burden. The analysis of beach-cast algae showed that the dominant species are macroalgae Ulva sp., Furcellaria lumbricalis, Cladophora sp., and Polysiphonia fucoides. The biomass of Furcellaria and Polysiphonia algae, containing 25.6% and 19.98% sugars, respectively, has the greatest resource potential in terms of obtaining carbohydrates. Fucose, glucose, and galactose were found to be the most common carbohydrates. The lipid content did not exceed 4.3% (2.3–4.3%), while the fatty acid composition was represented by saturated fatty acids (palmitic, stearic, methyloleic, behenic, etc.). The highest content of crude protein was found in samples of macroalgae of the genus Polysiphonia and amounted to 28.2%. A study of the elemental composition of drifting algae revealed that they have a high carbon content (31.3–37.5%) and a low hydrogen (4.96–5.82%), and sulfur (1.75–3.00%) content. Red algal biomass has the most resource potential in terms of biofuel generation, as it has a high number of lipids and proteins that can produce melanoidins during hydrothermal liquefaction, enhancing the fuel yield. The study noted the feasibility of using the biomass of the studied algae taxa to produce polysaccharides and biofuels. The analyses of antioxidant properties, fat content, and fat composition do not provide convincing evidence of the viability of using the aforementioned macroalgae for their production.
Numerical Modeling of Individual Plasma Dynamic Characteristics of a Light-Erosion MPC Discharge in Gases
Authors: Kuzenov Victor V. , Ryzhkov Sergei V. , Varaksin Aleksey Yu.
Published: April 2022

A mathematical model is formulated, and a numerical study of magneto-plasma compressor (MPC) discharges in gases for a wide range of changes in the main electrical parameters and the characteristics of the surrounding gas environment is carried out. The performed calculations showed, depending on the role of one or another heating mode (Ohmic, transient, and plasma dynamic), three different types of quasi-stationary spatial distributions of plasma parameters, which can be used to judge the features of the emerging structures and the dynamics of plasma propagation, and, therefore, to speak about the modes of discharge. The features of the radiation plasma dynamic structures and the change in the main parameters of the plasma of an MPC discharge in the transient regime are considered.
Particle Number Concentration: A Case Study for Air Quality Monitoring
Authors: Thén Wanda , Salma Imre
Published: April 2022

Particle matter is one of the criteria air pollutants which have the most considerable effect on human health in cities. Its legislation and regulation are mostly based on mass. We showed here that the total number of particles and the particle number concentrations in different size fractions seem to be efficient quantities for air quality monitoring in urbanized areas. Particle number concentration (N) measurements were realized in Budapest, Hungary, for nine full measurements years between 2008 and 2021. The datasets were complemented by meteorological data and concentrations of criteria air pollutants. The annual medians of N were approximately 9 × 103 cm−3. Their time trends and diurnal variations were similar to other large continental European cities. The main sources of N are vehicle road traffic and atmospheric new aerosol particle formation (NPF) and consecutive growth events. The latter process is usually regional, so it appears to be better assessible for contribution quantification than mass concentration. It is demonstrated that the relative occurrence frequency of NPF was considerable, and its annual mean was around 20%. NPF events increased the contribution of ultrafine (UF < 100 nm) particles with respect to the regional particle numbers by 12% and 37% in the city center and in the near-city background, respectively. The pre-existing UF concentrations were doubled on the NPF event days.
Occurrence and Discrepancy of Surface and Column Mole Fractions of CO2 and CH4 at a Desert Site in Dunhuang, Western China
Authors: Wei Chong , Lyu Zheng , Bu Lingbing , Liu Jiqiao
Published: April 2022

Carbon dioxide (CO2) and methane (CH4) are the two major radiative forcing factors of greenhouse gases. In this study, surface and column mole fractions of CO2 and CH4 were first measured at a desert site in Dunhuang, west China. The average column mole fractions of CO2 (XCO2) and CH4 (XCH4) were 413.00 ± 1.09 ppm and 1876 ± 6 ppb, respectively, which were 0.90 ppm and 72 ppb lower than their surface values. Diurnal XCO2 showed a sinusoidal mode, while XCH4 appeared as a unimodal distribution. Ground observed XCO2 and XCH4 were compared with international satellites, such as GOSAT, GOSAT-2, OCO-2, OCO-3, and Sentinel-5P. The differences between satellites and EM27/SUN observations were 0.26% for XCO2 and −0.38% for XCH4, suggesting a good consistency between different satellites and ground observations in desert regions in China. Hourly XCO2 was close to surface CO2 mole fractions, but XCH4 appeared to have a large gap with CH4, probably because of the additional chemical removals of CH4 in the upper atmosphere. It is necessary to carry out a long-term observation of column mole fractions of greenhouse gases in the future to obtain their temporal distributions as well as the differences between satellites and ground observations.
Data Analysis Using a Coupled System of Ornstein–Uhlenbeck Equations Driven by Lévy Processes
Authors: Mariani Maria , Asante Peter , Kubin William , Tweneboah Osei
Published: April 2022

In this work, we have analyzed data sets from various fields using a coupled Ornstein–Uhlenbeck (OU) system of equations driven by Lévy processes. The Ornstein–Uhlenbeck model is well known for its ability to capture stochastic behaviors when used as a predictive model. There’s empirical evidence showing that there exist dependencies or correlations between events; thus, we may be able to model them together. Here we show such correlation between data from finance, geophysics and health as well as show the predictive performance when they are modeled with a coupled Ornstein–Uhlenbeck system of equations. The results show that the solution to the stochastic system provides a good fit to the data sets analyzed. In addition by comparing the results obtained when the BDLP is a Γ(a,b) process or an IG(a,b) process, we are able to deduce the best choice out of the two to model our data sets.
Optimized Nail for Penetration Test on Lithium-Ion Cells and Its Utilization for the Validation of a Multilayer Electro-Thermal Model
Authors: Aiello Luigi , Gstrein Gregor , Erker Simon , Kaltenegger Bernhard , Ellersdorfer Christian , Sinz Wolfgang
Published: April 2022

Nail penetration is one of the most critical scenarios for a lithium-ion cell: it involves the superposition of electrical, thermal and mechanical abusive loads. When an electrically conductive nail is introduced into the active layers of a lithium-ion cell, an electric short circuit takes place between the conductive components (electrodes and current collectors). Hence, for this load case, electro-thermal modeling must be performed considering each and every layer of the cell in order to predict the electric quantities and the cell temperature (with numerical models). When standard conic nails are used, as is typical for this class of tests, the electrical contact between conductive components and the nail itself suffers of poor reproducibility mainly due to the separator that interposes between the electrically conductive components. This phenomenon makes it difficult to validate electro-thermal models, since the electrical contact between nail and lithium-ion cell parts cannot be safely determined. In this work, an alternative nail with an optimized ratio between the external surface and volume is presented to overcome this issue. To demonstrate the effectiveness of the designed nail, five tests (with the same conditions) were conducted on five commercial lithium-ion pouch cells, monitoring the tabs voltage and surface temperature. In all tests, thermal runaway was reached within 30 s and the tabs voltage showed comparable behavior, indicating that the short circuit values for all five repetitions were similar. The investigation included the implementation of a detailed layers model to demonstrate how the validation of such model would be possible with the novel data.
Operations with Nested Named Sets as a Tool for Artificial Intelligence
Authors: Burgin Mark
Published: April 2022

Knowledge and data representations are important for artificial intelligence (AI), as well as for intelligence in general. Intelligent functioning presupposes efficient operation with knowledge and data representations in particular. At the same time, it has been demonstrated that named sets, which are also called fundamental triads, instantiate the most fundamental structure in general and for knowledge and data representations in particular. In this context, named sets allow for effective mathematical portrayal of the key phenomenon, called nesting. Nesting plays a weighty role in a variety of fields, such as mathematics and computer science. Computing tools of AI include nested levels of parentheses in arithmetical expressions; different types of recursion; nesting of several levels of subroutines; nesting in recursive calls; multilevel nesting in information hiding; a variety of nested data structures, such as records, objects, and classes; and nested blocks of imperative source code, such as nested repeat-until clauses, while clauses, if clauses, etc. In this paper, different operations with nested named sets are constructed and their properties obtained, reflecting different attributes of nesting. An AI system receives information in the form of data and knowledge and processing information, performs operations with these data and knowledge. Thus, such a system needs various operations for these processes. Operations constructed in this paper perform processing of data and knowledge in the form of nested named sets. Knowing properties of these operations can help to optimize the processing of data and knowledge in AI systems.
Body Acoustics for the Non-Invasive Diagnosis of Medical Conditions
Authors: Cook Jadyn , Umar Muneebah , Khalili Fardin , Taebi Amirtahà
Published: April 2022

In the past few decades, many non-invasive monitoring methods have been developed based on body acoustics to investigate a wide range of medical conditions, including cardiovascular diseases, respiratory problems, nervous system disorders, and gastrointestinal tract diseases. Recent advances in sensing technologies and computational resources have given a further boost to the interest in the development of acoustic-based diagnostic solutions. In these methods, the acoustic signals are usually recorded by acoustic sensors, such as microphones and accelerometers, and are analyzed using various signal processing, machine learning, and computational methods. This paper reviews the advances in these areas to shed light on the state-of-the-art, evaluate the major challenges, and discuss future directions. This review suggests that rigorous data analysis and physiological understandings can eventually convert these acoustic-based research investigations into novel health monitoring and point-of-care solutions.
Small Dense LDL: Scientific Background, Clinical Relevance, and Recent Evidence Still a Risk Even with ‘Normal’ LDL-C Levels
Authors: Superko Harold , Garrett Brenda
Published: April 2022

Residual cardiovascular disease event risk, following statin use and low-density lipoprotein cholesterol (LDL-C) reduction, remains an important and common medical conundrum. Identifying patients with significant residual risk, despite statin drug use, is an unmet clinical need. One pathophysiologic disorder that contributes to residual risk is abnormal distribution in lipoprotein size and density, which is referred to as lipoprotein heterogeneity. Differences in low density lipoprotein (LDL) composition and size have been linked to coronary heart disease (CHD) risk and arteriographic disease progression. The clinical relevance has been investigated in numerous trials since the 1950s. Despite this long history, controversy remains regarding the clinical utility of LDL heterogeneity measurement. Recent clinical trial evidence reinforces the relevance of LDL heterogeneity measurement and the impact on CHD risk prediction and outcomes. The determination of LDL subclass distribution improves CHD risk prediction and guides appropriate treatment.
Alcohol-Induced Alterations in the Vascular Basement Membrane in the Substantia Nigra of the Adult Human Brain
Authors: Skuja Sandra , Jain Nityanand , Smirnovs Marks , Murovska Modra
Published: April 2022

The blood–brain barrier (BBB) represents a highly specialized interface that acts as the first line of defense against toxins. Herein, we investigated the structural and ultrastructural changes in the basement membrane (BM), which is responsible for maintaining the integrity of the BBB, in the context of chronic alcoholism. Human post-mortem tissues from the Substantia Nigra (SN) region were obtained from 44 individuals, then grouped into controls, age-matched alcoholics, and non-age-matched alcoholics and assessed using light and electron microscopy. We found significantly less CD31+ vessels in alcoholic groups compared to controls in both gray and white matter samples. Alcoholics showed increased expression levels of collagen-IV, laminin-111, and fibronectin, which were coupled with a loss of BM integrity in comparison with controls. The BM of the gray matter was found to be more disintegrated than the white matter in alcoholics, as demonstrated by the expression of both collagen-IV and laminin-111, thereby indicating a breakdown in the BM’s structural composition. Furthermore, we observed that the expression of fibronectin was upregulated in the BM of the white matter vasculature in both alcoholic groups compared to controls. Taken together, our findings highlight some sort of aggregation or clumping of BM proteins that occurs in response to chronic alcohol consumption.
Bacteria-Mediated Modulatory Strategies for Colorectal Cancer Treatment
Authors: Mueller Anna-Lena , Brockmueller Aranka , Fahimi Niusha , Ghotbi Tahere , Hashemi Sara , Sadri Sadaf , Khorshidi Negar , Kunnumakkara Ajaikumar B. , Shakibaei Mehdi
Published: April 2022

Colorectal cancer (CRC) is one of the most common tumors worldwide, with a higher rate of distant metastases than other malignancies and with regular occurrence of drug resistance. Therefore, scientists are forced to further develop novel and innovative therapeutic treatment strategies, whereby it has been discovered microorganisms, albeit linked to CRC pathogenesis, are able to act as highly selective CRC treatment agents. Consequently, researchers are increasingly focusing on bacteriotherapy as a novel therapeutic strategy with less or no side effects compared to standard cancer treatment methods. With multiple successful trials making use of various bacteria-associated mechanisms, bacteriotherapy in cancer treatment is on its way to become a promising tool in CRC targeting therapy. In this study, we describe the anti-cancer effects of bacterial therapy focusing on the treatment of CRC as well as diverse modulatory mechanisms and techniques that bacteriotherapy offers such as bacterial-related biotherapeutics including peptides, toxins, bacteriocins or the use of bacterial carriers and underlying molecular processes to target colorectal tumors.
Urinary Biomarkers for Detection of Clinical Endometriosis or Adenomyosis
Authors: Chen Wei-Chun , Cheng Chao-Min , Liao Wan-Ting , Chang Ting-Chang
Published: April 2022

Endometriosis or adenomyosis can be clinically diagnosed by ultrasound, symptoms, physical examination, and serum CA125. The urinary markers need to be investigated. The aim of our study was to investigate the urinary markers of clinical endometriosis/adenomyosis, and the correlation of serum CA125 was also studied. From the literature, alpha-1 antitrypsin (A1AT), enolase-1, vitamin D binding protein (VDBP), and CA125 in urine and serum were used in our study and measured by enzyme-linked immunosorbent assays (ELISA). Further clinical correlation and detection performance were evaluated. We enrolled 19 normal controls and 33 patients clinically diagnosed with endometriosis/adenomyosis. There were significant differences between studied patients and normal controls, as follows: serum CA125 (130.91 vs. 19.75 U/mL, p = 0.004); urinary CA125-creatinine ratio (5.591 vs. 0.254 ng/mg, p = 0.028); and urinary VDBP-creatinine ratio (28.028 vs. 7.301 ng/mg, p = 0.018). For diagnostic performances, serum CA125 provided the best results, with an area under curve (AUC) of 0.888 (p = 0.001) and accuracy of 86.5%. Other excellent results were also found using urinary VDBP (AUC 0.841, p = 0.001) and A1AT (AUC 0.722, p = 0.011) creatinine ratio. Using three combined biomarkers, serum CA125, urinary VDBP, and A1AT creatinine ratio, provided good detection power (AUC 0.913, p = 0.001, sensitivity 90.9%, specificity 76.5%). Double urine markers used in combination with VDBP and A1AT creatinine ratio also provided good diagnostic performance (AUC 0.809, p = 0.001, sensitivity 81.8%, specificity 76.5%, accuracy 80%). Further development of non-invasive point-of-care tests using these biomarkers could be a fruitful future endeavor.
Immunogenicity, Effectiveness, and Safety of COVID-19 Vaccines in Rheumatic Patients: An Updated Systematic Review and Meta-Analysis
Authors: Tang Kuo-Tung , Hsu Bo-Chueh , Chen Der-Yuan
Published: April 2022

Background: Vaccination is one of the most important measures worldwide to halt the spread of the corona virus disease 2019 (COVID-19). However, the efficacy and safety of these vaccines in rheumatic patients are not well explored. Therefore, we conducted a systematic review and meta-analysis. Methods: We performed a literature search of the PubMed and EMBASE databases on 17 November 2021. Forty-seven studies relevant to the immunogenicity, efficacy/effectiveness, and safety of COVID-19 vaccines were selected. Results: Our results demonstrated that COVID-19 vaccination is effective in protecting rheumatic patients from severe illness caused by the virus. Both the humoral and cellular immunogenicity of vaccines were impaired in rheumatic patients, which were greatly enhanced after the second vaccine dose. Receiving anti-CD20 therapy was associated with impaired humoral immunogenicity. Adverse events due to COVID-19 vaccines in rheumatic patients were similar to those in healthy controls, except for an increased incidence of arthralgia. The incidence of disease flares after COVID-19 vaccination was low. Conclusion: Our systematic review indicated the importance of full vaccination in rheumatic patients. Withholding anti-CD20 therapy was found to be potentially beneficial for the immunogenicity. Furthermore, the vaccines were found to be safe in general. Despite significant heterogeneity between studies, we recommend that rheumatic patients receive these vaccines amidst the global pandemic.
The Impact of Onset Age on Eosinophils in Kawasaki Disease
Authors: Chen Yu-Jhen , Guo Mindy Ming-Huey , Chang Ling-Sai , Kuo Ho-Chang
Published: April 2022

(1) Background: Kawasaki disease (KD) mainly affects children under the age of 5 years and eosinophilia in KD patients might be associated with the development of allergic diseases. We compared the age-adjusted Z-score (Z) of eosinophils and aimed to evaluate the impact of onset age on eosinophils in KD patients. (2) Methods: We divided 398 KD patients into seven age subgroups. Laboratory data and the age-adjusted Z-score of eosinophils during the phases of Kawasaki disease were analyzed. (3) Results: The absolute eosinophil count among all age groups showed significant differences in the post-intravenous immunoglobulin (IVIG) phase and throughout the course of KD with Z-score adjusted for age. Further analysis showed persistent elevation of the age-adjusted Z-score of eosinophils (Z-eosinophil) especially in the under six-month-old age subgroup. In addition, we divided the Z-eosinophil into two groups to find the relationship with coronary artery lesions (CALs). Patients with a higher eosinophil count than average age values had a higher risk of developing CALs, while those with a lower eosinophil count than average age values had a lower risk of having CALs. (4) Conclusions: These findings may provide information to clinicians to pay attention to allergic diseases during the follow-up of KD, especially for children who are younger than 6 months old at the onset of KD, and eosinophil count could be a crucial focus in KD.
The Role of Fecal Microbiota Transplantation in the Treatment of Acute Graft-versus-Host Disease
Authors: Biliński Jarosław , Jasiński Marcin , Basak Grzegorz W.
Published: April 2022

The number of allogeneic hematopoietic stem cell transplantations conducted worldwide is constantly rising. Together with that, the absolute number of complications after the procedure is increasing, with graft-versus-host disease (GvHD) being one of the most common. The standard treatment is steroid administration, but only 40–60% of patients will respond to the therapy and some others will be steroid-dependent. There is still no consensus regarding the best second-line option, but fecal microbiota transplantation (FMT) has shown encouraging preliminary and first clinically relevant results in recent years and seems to offer great hope for patients. The reason for treatment of steroid-resistant acute GvHD using this method derives from studies showing the significant immunomodulatory role played by the intestinal microbiota in the pathogenesis of GvHD. Depletion of commensal microbes is accountable for aggravation of the disease and is associated with decreased overall survival. In this review, we present the pathogenesis of GvHD, with special focus on the special role of the gut microbiota and its crosstalk with immune cells. Moreover, we show the results of studies and case reports to date regarding the use of FMT in the treatment of steroid-resistant acute GvHD.
Synthesis and Physicochemical Characterization of Polymer Film-Based Anthocyanin and Starch
Authors: Erna Kana Husna , Felicia Wen Xia Ling , Vonnie Joseph Merillyn , Rovina Kobun , Yin Koh Wee , Nur’Aqilah Md Nasir
Published: April 2022

Colorimetric indicators, used in food intelligent packaging, have enormous promise for monitoring and detecting food quality by analyzing and interpreting the quality data of packaged food. Hence, our study developed and characterized a biopolymer film based on starch and anthocyanin for prospective meat freshness monitoring applications. The developed film was morphologically characterized using different morphology instruments to identify the interaction between anthocyanin and starch. The color differences of the proposed film in response to different pH buffers have also been investigated. The combination of anthocyanin and starch produces a smooth and homogenous surface with an intermolecular hydrogen bond that increases the biopolymer’s wavelength. The film indicated bright red at pH 2.0–6.0, bluish-grey at pH 7.0–11.0, and yellowish-green above 11.0 that the naked eye can see. The indicator film shows high sensitivity toward pH changes. The inclusion of anthocyanin increases the biopolymer film’s thickness and crystalline condition with low humidity, water solubility, and swelling values. As a result, the polymer film can be employed in the food industry as an affordable and environmentally friendly indication of meat freshness.