bluuebunny/crossref_metadata_2025 huggingface dataset:
Rozszerzenie faktu historycznego w literaturze kontrfaktycznej i jego wartości dydaktyczne
Authors: Saja Krystian
Published: May 2024

Jednym z głównych celów historii kontrfaktycznych jest pełniejsze odczytywanie złożonych procesów dziejowych. Z pomocą literatury kontrfaktycznej budowany jest pełniejszy obraz przeszłości, a dopełnienie wiedzy o dziejach bieżących następuje za sprawą zrozumienia możliwych alternatyw. Przestrzeń historii alternatywnych buduje „nowe fakty”, ukazując wizje ich istnienia w procesie dziejowym. Innymi słowy, fakt historyczny, traktowany jako punkt przełamania, stanowi moment zwrotny w historii, w którym za sprawą literatury kontrfaktycznej realizuje się odmienny scenariusz dziejów. Historyk jest zobowiązany połączyć szereg faktów w ciąg przyczynowo-skutkowy, zespolony jednością wewnętrznej konieczności. Autor historii alternatywnej stwarza świat nowy, odrealniony, a jednocześnie prawdziwy, logiczny, mający początek w fakcie historycznym, zaś koniec osadzony w fikcji lub spekulacji, z konkluzją: „tak mogło być naprawdę”. Tak rozumiane historie alternatywne otwierają nowe możliwości w zakresie dydaktyki. Narracja historyczna osadzona w ramach powieści staje się atrakcyjniejszą formą przekazu dla młodego pokolenia. Ukazanie alternatywnych wariantów dziejowych poszerza nie tylko przestrzeń wyobraźni, ale przede wszystkim abstrakcyjnego i logicznego myślenia, z poszanowaniem historii.
Wireless substitution: Early Release of Estimates From the National Health Interview Survey, July–December 2023
Authors: Blumberg Stephen J. , Luke Julian V.
Published: May 2024

This report provides health data from the National Center for Health Statistics.
Dickeya zeae, Pantoea ananatis, and Xanthomonas vasicola pv. vasculorum: Control with the use of nanoparticles
Authors: Mamede Morgana Coelho , Silva Anielle Christine Almeida , Tebaldi Nilvanira Donizete
Published: May 2024

ABSTRACT Metal oxide nanoparticles (NPs) possess antibacterial activities and can be used for the control of phytopathogenic bacteria. The objective of this work was to evaluate the antibacterial activity of pure and doped NPs against Dickeya zeae, Pantoea ananatis, and Xanthomonas vasicola pv. vasculorum in vitro, and to assess the efficacy of preventive and curative application of zinc oxide (ZnO), nickel oxide (NiO), and silver-doped zinc oxide (ZnO:Ag) NPs for the control of white spot (WS) and bacterial leaf streak (BLS) in vivo. Bacterial growth inhibition was first evaluated by measuring the diameter of the inhibition zone formed in Petri dishes. Subsequently, the severity of WS and BLS diseases was evaluated in a greenhouse calculating the area under the disease progress curve. The in vitro antibacterial activity was not influenced by the increase in the concentration of doping elements for most NPs. ZnO NPs doped with Ag, K, and Mo; ZnOCl doped with Ag, and pure NPs (Ag2O, CuO, and NiO) showed antibacterial activity against D. zeae, P. ananatis, and X. vasicola pv. vasculorum with relatively similar inhibition zones at different concentrations. Commercial copper showed antibacterial activity only against D. zeae. NiO NPs in preventive and curative applications reduced WS and BLS severities, whereas commercial copper application increased WS severity and reduced BLS severity. The use of NPs has promising applications and further evaluation of their formulation, application form, and timing is necessary for new strategies to control the activity of phytopathogenic bacteria.
Análise da influência de metal de adição e gás de proteção em juntas soldadas de aço galvanizado
Authors: Fensterseifer Ricardo Augusto , Haupt William
Published: May 2024

RESUMO A ampla adoção de aço galvanizado no segmento industrial, como a indústria automotiva, baseia-se em ótimas propriedades em conferir durabilidade aos produtos manufaturados, além de reduzir impactos ambientais e custos na cadeia produtiva, evitando-se corrosão e conferindo maior vida útil devido a camada protetora de zinco. Porém, essa camada quando submetida a processo de soldagem implica potenciais defeitos devido à evaporação de vapor de zinco e fragilidade do metal líquido durante a soldagem, com potencial geração de porosidade, falta de penetração ou fusão. Em última instância, esses defeitos podem trazer impactos significativos na redução de propriedades mecânicas, trincas e fraturas nas juntas soldadas. Neste trabalho, essas implicações são analisadas através das características microestruturais e propriedades mecânicas de juntas soldadas com aço galvanizado ZAR230 pelos processos de soldagem GMAW e FCAW, avaliando-se fatores mais amplamente modificados em escala industrial nessa técnica de soldagem: o tipo de metal de adição, sendo ele arame sólido ou tubulares – autoprotegido com proteção gasosa e sem proteção gasosa, e o tipo de gás de proteção (com misturas de 92% Ar + 8% CO2, e 95% Ar + 5% O2). Utilizando-se de técnicas de microscopia ótica de varredura, metalografia, ensaios de tração e microdureza, os resultados apontam que todas as condições testadas possuem propriedades mecânicas de dureza e tração aceitáveis, com variação de presença de defeitos, inclusões e aspecto de cordão, sendo a melhor combinação a de uso do arame sólido com mistura 92% Ar + 8% CO2.
Neonatal innate immunity response in invasive candidiasis
Authors: Suárez J. A. G. , Calumby R. J. N. , Silva D. P. , Barbosa V. T. , Maranhão F. C. A. , Moreira I. F. , Melhem M. S. C. , Moreira R. T. F.
Published: May 2024

Abstract Infections caused by Candida spp. are frequent in critically hospitalized patients, especially among premature neonates, representing one of the most common healthcare-related infections. Although there is considerable production of current knowledge about the mechanisms of immune response, aspects involved in the newborn's innate defense are not fully understood. The aim of this study was to describe the innate immune mechanisms involved in the defense of neonates against invasive candidiasis. This is an integrative literature review from the Scopus, Scifinder, Medline, Web of Science databases and the electronic libraries ScienceDirect and Scielo, in the period between 2002 and 2020, with rescue based on primary descriptor Immunity Innate plus secondary descriptors Candidiasis Invasive AND Infant Newborn. We have observed the involvement of various mechanisms in the neonatal response against invasive candidiasis, including the recognition, signaling, recruitment, and initiation of an effective immune response. These mechanisms encompass the presence of antimicrobial peptides, phagocytosis, synthesis of reactive oxygen species, inflammatory mediators, and complex cell signaling systems mediated by Pattern Recognition Receptors (PRRs). With this study, it is expected to contribute to the expansion of knowledge about the immunological mechanisms involved in the innate immune response of the newborn against disseminated infections caused by Candida species, and in the same sense, highlight the importance of this knowledge as a reflex in the decrease in mortality in the neonatal period.
Comparative study of the quality of breast meat of free-range chicken (Gallus gallus domesticus) and conventional chicken (Gallus gallus)
Authors: Lopes C. , Cardoso F. A. R. , Mendes P. S. , Droval A. A.
Published: May 2024

Abstract This study compares the physicochemical characteristics of breast meat (Pectoralis major) from conventional chicken and free-range chicken production systems. Analyses of pH, instrumental color measurement, weight loss from cooking (WLC), and water retention capacity (WRC) were carried out. Average pH values were slightly higher for conventional chicken samples. WLC did not show a significant difference between conventional and free-range chicken samples. The WRC was better and higher for the free-range chicken samples than the conventional ones. The mean values for luminosity (L*) were within the normal range, with slightly higher values for conventional chicken. In chromatids a* and b*, there was a tendency towards a more reddish color for free-range chicken samples. The differences found for types of production can be explained mainly by the difference in age at slaughter, the degree of physical activity, animal feeding, among other characteristics that differentiate an animal raised by the extensive system from the intensive system.
Determination of fumonisin content in maize using near-infrared hyperspectral imaging (NIR-HSI) technology and chemometric methods
Authors: Conceição R. R. P. , Queiroz V. A. V. , Medeiros E. P. , Araújo J. B. , Araújo D. D. S. , Miguel R. A. , Stoianoff M. A. R. , Simeone M. L. F.
Published: May 2024

Abstract Maize (Zea mays L.) is of socioeconomic importance as an essential food for human and animal nutrition. However, cereals are susceptible to attack by mycotoxin-producing fungi, which can damage health. The methods most commonly used to detect and quantify mycotoxins are expensive and time-consuming. Therefore, alternative non-destructive methods are required urgently. The present study aimed to use near-infrared spectroscopy with hyperspectral imaging (NIR-HSI) and multivariate image analysis to develop a rapid and accurate method for quantifying fumonisins in whole grains of six naturally contaminated maize cultivars. Fifty-eight samples, each containing 40 grains, were subjected to NIR-HSI. These were subsequently divided into calibration (38 samples) and prediction sets (20 samples) based on the multispectral data obtained. The averaged spectra were subjected to various pre-processing techniques (standard normal variate (SNV), first derivative, or second derivative). The most effective pre-treatment performed on the spectra was SNV. Partial least squares (PLS) models were developed to quantify the fumonisin content. The final model presented a correlation coefficient (R2) of 0.98 and root mean square error of calibration (RMSEC) of 508 µg.kg-1 for the calibration set, an R2 of 0.95 and root mean square error of prediction (RMSEP) of 508 µg.kg-1 for the test validation set and a ratio of performance to deviation of 4.7. It was concluded that NIR-HSI with partial least square regression is a rapid, effective, and non-destructive method to determine the fumonisin content in whole maize grains.
Prediction of essential oil content in C. sintoc Leaves based on the direction of vegetation slope in Mount Ciremai National Park using ANFIS Artificial Neural Network
Authors: Ismail A. Y. , Kusmana C. , Nainggolan M. F. , Gandaseca S.
Published: May 2024

Abstract C. sintoc is a plant that has a high essential oil content. Essential oils have many health benefits. Mount Ciremai National Park is an area that has abundant vegetation, especially C. sintoc. The purpose of this study was to predict the volume of oil contained in the leaves of C. sintoc based on its growing location in Mount Ciremai National Park (TNGC), West Java. Sampling was carried out in two stages, namely field samples and laboratory samples. Field samples with a single plot measuring 20 x 20 meters. Extraction of laboratory samples by steam distillation method. Data analysis using ANFIS method to predict the volume of essential oil. The results showed that the volume of essential oil in the leaves was largest on the western slope (1,96 ml), northern slope (2,01 ml), eastern slope (1,55 ml) and southern slope (1,37 ml) while the essential oil yield of extract oil in the leaves is found on the western slope (0,08%), northern slope (0,07 %), eastern slope (0,06 %) and southern slope (0,04), On the western slope (1,82 ml), northern slope (1,73 ml). The ANFIS analysis results showed a prediction accuracy of 90,99% with the highest tree productivity when C. sintoc BL grows at an altitude of 650-700 meters above sea level, tree diameter of 42 cm, height of 12 m, growing on sand-textured land and at a humidity of 75 and at a growing temperature range of 25-270C. The value of essential oil production volume is influenced by each variable such as tree diameter, tree height, soil texture, altitude, temperature and humidity.
Detection of multiple circulating sandflies species and investigation of dogs and vectors naturally infected with Leishmania in the city of Ribeirão Vermelho, southeastern Brazil
Authors: Alvarenga Ingrid M. , Castro Joseane C. , Melo Leandro M.R. , Oliveira Mariana M. , Castro Pedro H. , Milagres Tarcísio F. , Andrade Filho José D. , Souza Camila B.S. , Fujiwara Ricardo T. , Barçante Thales A. , Peconick Ana P. , Barçante Joziana M.P.
Published: May 2024

ABSTRACT: Leishmaniasis is a zoonotic disease caused by parasites of the Leishmania genus, resulting in various clinical forms, including a highly lethal visceral form. This study aimed to identify Leishmania spp. in sandflies and dogs in a small Brazilian town. DPP® Visceral Canine Leishmaniasis (CVL) was used for screening, and ELISA and conventional PCR were used for confirmatory testing, while sandflies were captured using CDC light traps and conventional PCR targeting ITS1. The Hill series was used to identify the diversity profile of species in the sampled area using R software. The study identified a Leishmania spp. prevalence of 4.02% in dogs, exceeding the 2% limit required by the Brazilian Ministry of Health. A total of 443 sandflies belonging to 14 different species were identified, with Lutzomyia longipalpis being the most abundant (73.81%). Negligence regarding leishmaniasis in small towns can lead to late diagnosis, hence the need to implement effective strategies, including early diagnosis and treatment of human and canine cases, vector control programs, and awareness campaigns to educate the public about risks and preventive measures. These measures can help prevent the spread of leishmaniasis and improve health outcomes for affected individuals and animals.
Os efeitos da proibição das coligações proporcionais no comportamento dos atores
Authors: Schaefer Bruno Marques , Krause Silvana , Mancuso Wagner Pralon
Published: May 2024

RESUMO Introdução: Estudos mostraram que a proibição de coligações partidárias em eleições proporcionais reduziu a fragmentação partidária nas Câmaras Municipais no Brasil em 2020. Todavia, eles se concentraram apenas no efeito da nova regra legal sobre o sistema partidário em nível local. Este artigo faz uma análise alternativa a fim de entender: 1) a influência do resultado eleitoral dos partidos em 2016 nas suas decisões de lançar candidatos em 2020; 2) o impacto do desempenho eleitoral de 2016 na troca de partidos por candidatos em 2020; e 3) se as diferentes estratégias das elites políticas (no caso, tanto lideranças partidárias como candidatos) alteraram o grau de fragmentação partidária. Materiais e métodos: Utilizamos dados do desempenho partidário e individual nas eleições proporcionais municipais de 2016 e 2020 contabilizando três unidades de análise: município, partido e candidato. Utilizamos métodos estatísticos variados para estimar o impacto da proibição das coligações proporcionais sobre o comportamento dos atores tais como regressão logística binária, multinomial e regressão linear. Resultados: Os resultados demonstram que quanto melhor o desempenho do partido em 2016 maior foi a probabilidade de lançar candidatos em 2020. Isso diminuiu também a probabilidade dos candidatos deixarem o partido pelo qual haviam concorrido anteriormente. Por fim, a interação entre estratégias tanto de partidos como de candidatos afetou de forma significativa a fragmentação partidária medida pelo Número Efetivo de Partidos. Discussão: Esses achados contribuem para avaliarmos de forma substantiva os efeitos do fim das coligações proporcionais no Brasil. Além de demonstrar que houve redução da fragmentação partidária, demonstramos que esse efeito é mediado pelo comportamento das elites políticas, seja na decisão de lançar ou não candidatos (no nível do partido), seja na decisão de migrar ou de permanecer no mesmo partido (no nível do candidato).
Home caregiver strategies for feeding older adults with dysphagia after dehospitalization
Authors: Belmonte Mariana Souza , Pedreira Larissa Chaves , Gomes Nildete Pereira , Oliveira Daniele Vieira , Souza Ana Carla Filgueira de Souza e , Pinto Ivana Santos
Published: May 2024

ABSTRACT Objective: To understand caregivers’ strategies for offering food to older adults with oropharyngeal dysphagia after dehospitalization. Method: Qualitative research carried out with caregivers of older adults with oropharyngeal dysphagia, who were discharged after hospitalization at a university hospital in Bahia. Data collection was carried out between January and February 2023 through a semi-structured interview, whose data were organized based on content analysis and analyzed with the help of IRaMuTeQ software. Results: Three categories emerged: Caregivers’ strategies for safely offering food to older adults with dysphagia; Caregiver strategies for oral hygiene for older adults; Recognition of continuity of speech therapy after dehospitalization. Conclusion: Caregivers’ strategies for offering food to older adults with oropharyngeal dysphagia were supported by tacit knowledge and effective care in the hospital-home transition.
Estratégias de cuidadores domiciliares para alimentação de pessoas idosas com disfagia após desospitalização
Authors: Belmonte Mariana Souza , Pedreira Larissa Chaves , Gomes Nildete Pereira , Oliveira Daniele Vieira , Souza Ana Carla Filgueira de Souza e , Pinto Ivana Santos
Published: May 2024

RESUMO Objetivo: Apreender as estratégias de cuidadores para oferta do alimento à pessoa idosa com disfagia orofaríngea após desospitalização. Método: Pesquisa qualitativa, realizada com cuidadores de pessoas idosas com disfagia orofaríngea, que tiveram alta após internamento em um hospital universitário na Bahia. A coleta de dados foi realizada entre janeiro e fevereiro de 2023, por meio de uma entrevista semiestruturada, cujo dados foram organizados com base na análise de conteúdo e analisados com auxílio do software IRaMuTeQ. Resultados: Emergiram três categorias: Estratégias dos cuidadores para oferta segura do alimento à pessoa idosa com disfagia; Estratégias de cuidadores para higiene oral da pessoa idosa; Reconhecimento da continuidade do acompanhamento fonoaudiológico após desospitalização. Conclusão: As estratégias dos cuidadores para a oferta do alimento a pessoas idosas com disfagia orofaríngea foram sustentadas pelo conhecimento tácito e por um cuidado efetivo na transição hospital-domicílio.
CREATION AND CONTENT VALIDITY OF 5R-MEDSAFE: ASSESSING ADHERENCE TO THE SAFE DRUG ADMINISTRATION “FIVE RIGHTS”
Authors: Andolhe Rafaela , Padilha Adriel , Guirardello Edineis de Brito , Gallani Maria Cecília Bueno Jayme , Rodrigues Roberta Cunha Matheus
Published: May 2024

ABSTRACT Objective: to present the creation and content validity stages of a questionnaire to assess the determinants of adherence to the safe drug administration five “rights” “x”, based on the Theory of Planned Behavior integrative model. Method: a methodological study to create and validate a self-reported measuring instrument for psychosocial variables. It took place in two public university teaching hospitals: one located in the South and the other in the Southeast of Brazil. Results: the results were organized according to each stage of the 5R-MEDSAFE content validation process. Conclusion: the results obtained in this creation and content validation study of the 5R-MEDSAFE indicated that the tool presented diverse content validity evidence. Its application can be useful in different contexts as a way of assessing adherence to these behaviors among Nursing workers. This will make it possible to identify which elements of the behaviors are amenable to intervention, as well as to implement the most appropriate intervention, according to the Theory of Planned Behavior constructs.
PREVENTION AND CONTROL OF INFECTION RELATED TO PERIPHERAL ARTERIAL CATHETER MANAGEMENT
Authors: Pereira Vitória Helena , Cruz Maria Cristina Mendes de Almeida , Galon Tanyse , Januário Gabriela da Cunha , Contim Divanice , Santos Mariana Alvina dos , Monteiro Damiana Aparecida Trindade , Toffano Silmara Elaine Malaguti
Published: May 2024

ABSTRACT Objective: to describe scientific evidence on good practices for peripheral arterial catheter management. Method: this is an integrative review, carried out through a search in the Latin American and Caribbean Literature in Health Sciences, Virtual Health Nursing Library, National Library of Medicine, Cochrane Library, Cumulative Index to Nursing & Allied Health, Excerpta Medica dataBASE, SciVerse Scopus TopCited and Web of Science databases in March 2021. Articles in Portuguese, English and Spanish, without time limits in the search, were included. Results: forty-nine articles were found. At insertion, measures involved hand hygiene, skin preparation, no-touch technique, aseptic technique and protective barrier, sterile components and transducers, peripheral arterial catheter insertion, insertion attempts, ultrasound and comfort measure use. During maintenance, issues regarding insertion site, invasive blood pressure circuit, connectors, dressing and stabilization were identified, and, during removal, aspects such as local and systemic complications after peripheral arterial catheter removal. Conclusion: the study provides crucial information for the effective management of peripheral arterial catheters, contributing to the reduction of complications and improvement of clinical results. By updating their practices, healthcare professionals can ensure greater safety and well-being for patients, always seeking to provide excellent care.
Organizing in the Ruins: Contributions of Multispecies Approaches in Administration
Authors: Leite Maria Clara de Oliveira , Fantinel Letícia Dias
Published: May 2024

ABSTRACT Objective: this essay problematizes destructive organizational practices that produce and perpetuate spaces in ruins. Drawing from the practices of the mining industry in Brazil, exemplified by the rupture of the Fundão dam (MG), we theorize about the contributions of multispecies approaches to Administration. Thesis: we propose, through dialogues between organizational studies and multispecies studies, the concept of a multispecies organizing of space that transcends exclusively human relations. We consider space as both product and process in everyday organizational life, yet we highlight the limitations of the human-centric focus in the literature. We conceptualize spacing as a non-human production of practices entangling humans and actors composing environments and various species of plants, animals, fungi, and other forms of existence. Thus, we challenge a hegemonic view in the field, underscoring the importance of a more inclusive and ethical approach to spatial and organizational practices. Conclusions: we rethink social and organizational dynamics, emphasizing not only mining activity but other corporate practices through a spatialized and more-than-human perspective. The essay contributes ontologically by making visible the agency of other living beings in organizational processes and practices, methodologically by decentering the human in research, and politically by analyzing asymmetric power relations. Such contributions enable the promotion of a broader and more responsible understanding of the complex relations between humans and non-humans in organizational contexts.
Organizar em Ruínas: Contribuições de Abordagens Multiespécies na Administração
Authors: Leite Maria Clara de Oliveira , Fantinel Letícia Dias
Published: May 2024

RESUMO Objetivo: este ensaio problematiza práticas organizativas destrutivas que produzem e reproduzem espaços em ruínas. Partimos das práticas da indústria mineradora no Brasil, materializadas no rompimento da barragem de Fundão (MG), para teorizar sobre contribuições de abordagens multiespécies para a Administração. Tese: propomos, a partir de diálogos entre estudos organizacionais e estudos multiespécies, a ideia de um organizar multiespécies do espaço que extrapola relações exclusivamente humanas. Consideramos o espaço produto e processo no cotidiano organizacional, mas salientamos as limitações do foco no espacializar humano na literatura. Tratamos o espacializar como uma produção mais que humana de práticas que emaranham seres humanos e atores que compõem ambientes e diferentes espécies de plantas, animais, fungos e outras formas de existência. Com isso, desafiamos certa visão hegemônica na área, sublinhando a importância de uma abordagem mais inclusiva e ética nas práticas espaciais e organizativas. Conclusões: repensamos dinâmicas sociais e organizativas, enfatizando não apenas a atividade mineradora, mas outras práticas corporativas sob uma perspectiva espacializada e mais que humana. O ensaio contribui ontologicamente ao visibilizar a agência de outros seres vivos nos processos e práticas organizativas, metodologicamente ao descentrar o humano na pesquisa e politicamente ao analisar relações assimétricas de poder. Tais contribuições permitem promover uma compreensão mais ampla e responsável das complexas relações entre seres humanos e não humanos em contexto organizacional.
Síndrome congênita do Zika vírus: o papel da atenção primária e da rede de saúde
Authors: Cabral Karl Marx da Nóbrega , Coêlho Bernadete Perez , Miranda Gabriella Morais Duarte de
Published: May 2024

Resumo O artigo objetiva compreender a rede de atenção e os desafios que as crianças com síndrome congênita do Zika vírus (SCZ) e seus cuidadores enfrentam para garantir o cuidado integral. Trata-se de estudo qualitativo, parte de pesquisa desenvolvida no Hospital das Clínicas da Universidade Federal de Pernambuco, que acompanhou 151 crianças com suspeita para SCZ. Foram realizados dois grupos focais, um com trabalhadores de saúde e outro com cuidadores. As crianças com SCZ percorrem longas distâncias, frequentando diversos serviços da rede. Observou-se a dificuldade de condução e de reconhecimento do papel de equipe de referência da APS, resultando em um cuidado fracionado, com obstáculos à comunicação entre serviços e ausência de atenção às cuidadoras. Percebeu-se uma rede fragilizada, com encontros fragmentados e olhar reduzido. É fundamental que os esforços priorizem o fortalecimento do vínculo entre os nós da rede de atenção e apoio. Os atributos essenciais e derivados da APS são determinantes para a rede de serviços e a oferta de cuidados às crianças com a SCZ e seus familiares.
The implementation of intersectoral actions to assist children with congenital Zika virus syndrome in the state of Rio de Janeiro
Authors: Siqueira Sandra Aparecida Venâncio de , Hollanda Eliane , Beserra Ingrid Karla da Nóbrega , Lobato Lenaura , Vaitsman Jeni
Published: May 2024

Abstract This paper focuses on the actions and partnerships established between members of the State Secretariats of Health and Social Assistance in Rio de Janeiro and other organizations in the health and assistance areas to respond to the health emergency triggered by the congenital Zika virus syndrome between 2015 and 2018. Some characteristics of the context were: a severe economic crisis, the erosion of the governance capacities of the state political authorities as well and scarce technical-scientific knowledge about the epidemic. The study was grounded on an analysis of documents related to the topic, 17 semi-structured interviews with managers and professionals from the two secretariats. Interview scripts focused on intersectoral actions and processes of cooperation and coordination within and between organizations. The analytical grid included aspects of the interpersonal, informational, and decision-making roles of managers and technicians. The results show that, despite the political and economic crisis, the Secretariat actors managed to create a flow of care for patients. Informal cooperation and coordination mechanisms were crucial for the creation of long-term intersectoral strategies.
Vacina contra zika: desenvolvimento, agenciamentos e controvérsias sociotécnicas
Authors: Silva Lenir Nascimento da , Dias Francine de Souza
Published: May 2024

Resumo O ensaio teórico discute as controvérsias sobre o desenvolvimento da vacina contra a zika, evidenciando as negociações que envolvem as escolhas técnico-científicas e os efeitos da definição do “perfil do produto alvo da vacina” (TPP) para uso somente no cenário emergencial. São propostas três perspectivas de análise em diálogo com os Estudos Sociais da Ciência: os fluxos de estabelecimento de normativas pela Organização Mundial da Saúde (OMS), as narrativas publicadas em revistas especializadas e de um grupo de entrevistados. Concluímos que os termos de definição do TPP ajudaram a constituir a política ontológica da OMS, implicando: exposição, responsabilização e culpabilização de mulheres pela prevenção da síndrome congênita da zika; instituição de certas estratégias de vacinação; invisibilização de outros cenários possíveis; maior espaço de aceitação de determinadas plataformas; ampliação das desigualdades globais. Tal política ontológica engendrou uma potente racionalidade emergencial que distinguiu a vacina da necessidade social da vacinação, empurrando a última para a invisibilidade.
Reproductive Justice Lessons for Catalyzing Advocacy Strategies during Zika Crisis in Brazil
Authors: Brito Luciana , Rondon Gabriela
Published: May 2024

Abstract This article aims to revisit a constellation of responses to guarantee reproductive justice for women and girls during the Zika crisis in Brazil, that were conducted by Anis - Institute of Bioethics, a Brazilian feminist NGO. We argue that intersectional feminist lenses and gender-sensitive responses are necessary to build effective efforts for women and girls during a public health emergency. As such, we present three concomitant and intersectional learned tactics we used to fight for reproductive justice during the Zika crises, but also in its aftermath: 1. To build storytelling narratives that portray the disproportional effects of the crisis on women and girls; 2. To produce evidence-based data to catalyze advocacy strategies for legal and policy review; 3. To promote movement building opportunities and sharing power through community mobilization activities. We assume the importance of providing immediate evidence and gender sensitive framings to inform real-time public health responses. Advocacy efforts should not be seen as fragmented strategies, since ensuring reproductive justice demands a comprehensive and transformative framework that include solutions for multiple aspects of real-life experiences.
Associations of mothers and family members affected by congenital Zika virus syndrome: profile and main related discussions
Authors: Valente Polyana Aparecida , Antunes Michele Nacif , Matos Silvana Sobreira de , Avery Lauren , Pimenta Denise Nacif
Published: May 2024

Abstract No Brasil, o impacto de emergências sanitárias consecutivas nos últimos cinco anos, como a epidemia de zika (2015) e a pandemia de COVID-19 (2020), atingiu de forma aguda as famílias de crianças com síndrome congênita do zika (SCZ). Este artigo explora processos epidêmicos que replicam as intersecções de deficiência, cuidado, gênero, classe e raça, e propõe a construção de um perfil das associações civis de mães e familiares de crianças com SCZ, bem como suas mobilizações, sobretudo da União Mães de Anjos (UMA). A partir da literatura sobre o tema, realização de entrevistas e oficinas com as mães de crianças com SCZ ligadas às associações em diferentes partes do Brasil, observou-se que as epidemias produzem impactos desiguais em relação a idade, raça, classe, gênero, localidade e deficiência. Embora os impactos das emergências de saúde possam ser contínuos para as pessoas que vivem na intersecção de múltiplas opressões, destacamos a resistência na forma de capacidade organizacional para navegar no acesso a direitos e reconfigurar dinâmicas de poder.Abstract: In Brazil, the impact of consecutive health emergencies in the last five years, such as the Zika epidemic (2015) and the COVID-19 pandemic (2020), acutely affected families of children with congenital Zika syndrome (CZS). This article explores epidemic processes that replicate the intersections of disability, care, gender, class and race, and proposes the construction of a profile of civil associations of mothers and families of children with CZS, as well as their mobilizations, especially the União Mães de Anjos (UMA). Based on the literature on the topic, interviews and workshops with mothers of children with CZS linked to associations in different parts of Brazil, it was observed that epidemics produce unequal impacts in relation to age, race, class, gender, location and disability. While the impacts of health emergencies can be ongoing for people living at the intersection of multiple oppressions, we highlight resistance in the form of organizational capacity to navigate access to rights and reconfigure power dynamics.
Associações de mães e familiares afetados pela síndrome congênita de Zika vírus: perfil e principais discussões relacionadas
Authors: Valente Polyana Aparecida , Antunes Michele Nacif , Matos Silvana Sobreira de , Avery Lauren , Pimenta Denise Nacif
Published: May 2024

Resumo No Brasil, o impacto de emergências sanitárias consecutivas nos últimos cinco anos, como a epidemia de zika (2015) e a pandemia de Covid-19 (2020), atingiu de forma aguda as famílias de crianças com síndrome congênita do zika (SCZ). Este artigo explora processos epidêmicos que replicam as intersecções de deficiência, cuidado, gênero, classe e raça, e propõe a construção de um perfil das associações civis de mães e familiares de crianças com SCZ, bem como suas mobilizações, sobretudo da União Mães de Anjos (UMA). A partir da literatura sobre o tema, realização de entrevistas e oficinas com as mães de crianças com SCZ ligadas às associações em diferentes partes do Brasil, observou-se que as epidemias produzem impactos desiguais em relação a idade, raça, classe, gênero, localidade e deficiência. Embora os impactos das emergências de saúde possam ser contínuos para as pessoas que vivem na intersecção de múltiplas opressões, destacamos a resistência na forma de capacidade organizacional para navegar no acesso a direitos e reconfigurar dinâmicas de poder.
Fim de emergências de saúde inacabadas e as redes de cuidados com a síndrome congênita do Zika vírus
Authors: Scott Russell Parry
Published: May 2024

Resumo Entendendo que problemas de saúde sempre são inacabadas, este trabalho examina consequências da declaração do fim de uma emergência de saúde sobre práticas de diferentes redes de cuidado interconectadas. Parte da pesquisa “Etnografando Cuidados...”, é um estudo de caso qualitativo de três documentos produzidos depois do anúncio do fim da epidemia da síndrome congênita de Zika vírus. Mostra contextos de produção de narrativas envolvendo redes de pesquisadores, gestores/prestadores de serviço, mães e famílias de acometidos e associações de mães e suas perspectivas diferentes sobre o que é cuidado. Análises de uma apresentação para pesquisadores e de um texto de discussão no IPEA questionam a narrativa técnica da celebração do fim da emergência com base em conhecimento e atendimento, sem tomar em conta a importância dos cuidados relacionais e afetivos e políticos (das redes de mães/familiares e de associações), deixando-os invisibilizados. Descreve o processo da elaboração da moção para o Fórum Zika na Pandemia, elencando e sistematizando propostas de ações através de um diálogo explícito entre integrante das diferentes redes para abordar questões inacabadas pós-emergenciais. Sugere que práticas semelhantes de diálogo entre redes possam promover maior inclusão e sensibilidade a cuidados que contribuem para diminuir sofrimento e defender direitos de pessoas que continuam a conviver cotidianamente com uma síndrome ou doença cujas consequências persistem.
Estratégia materna de enfrentamento à confirmação de infecção pelo vírus Zika na gravidez: reflexo no neurodesenvolvimento dos seus filhos
Authors: Costa Roozeméria Pereira , Méio Maria Dalva Barbosa Baker , Junqueira-Marinho Maria de Fátima , Moreira Maria Elisabeth Lopes
Published: May 2024

Resumo A infecção pelo vírus Zika na grávida resulta em alterações do desenvolvimento neuropsicomotor nas crianças afetadas, sendo importante fator de estresse para essas mulheres. O objetivo deste estudo foi avaliar a estratégia de enfrentamento das mães a essa situação e como isto se refletiu no neurodesenvolvimento dos seus filhos. Estudo transversal com 46 mulheres e seus filhos. A estratégia de enfrentamento foi avaliada pelo Inventário Brief Cope, aplicado às mães, e o desenvolvimento neuropsicomotor das crianças, foi avaliado aos 24 meses de idade pelas Escalas Bayley III. A estratégia predominante de enfrentamento mais frequentemente usada pelas mães foi a de aproximação (73,9%), com destaque para o componente planejamento. A utilização da estratégia de negação esteve associada aos escores mais baixos na escala Bayley III, sendo nessa escala o componente mais utilizado o de auto culpabilização. A religião foi o componente de apoio auxiliar mais utilizado pelas mães. A utilização da negação como estratégia predominante de enfrentamento pelas mães mostrou associação com os piores resultados na avaliação do desenvolvimento infantil e reforça a necessidade do apoio a estas mulheres, para que possam lidar mais diretamente com os sentimentos decorrentes das situações vivenciadas.
Targeting the Depletion of M2 Macrophages: Implication in Cancer Immunotherapy
Authors: Festekdjian Talia , Bonavida Benjamin
Published: May 2024

We have witnessed the emergence of immunotherapy against various cancers that resulted in significant clinical responses and particularly in cancers that were resistant to chemotherapy. These milestones have ignited the development of novel strategies to boost the anti-tumor immune response for immune-suppressed tumors in the tumor microenvironment (TME). Tumor-associated macrophages (TAMs) are the most abundant cells in the TME, and their frequency correlates with poor prognosis. Hence, several approaches have been developed to target TAMs in effort to restore the anti-tumor immune response and inhibit tumor growth and metastasis. One approach discussed herein is targeting TAMs via their depletion. Several methods have been reported for TAMs depletion including micro-RNAs, transcription factors (e.g., PPARγ, KLF4, STAT3, STAT6, NF-κB), chemokines and chemokine receptors, antibodies-mediated blocking the CSF-1/CSF-1R pathway, nanotechnology, and various combination treatments. In addition, various clinical trials are currently examining the targeting of TAMs. Many of these methods also have side effects that need to be monitored and reduced. Future perspectives and directions are discussed.
The Role of TAMs in the Regulation of Tumor Cell Resistance to Chemotherapy
Authors: McWhorter Ryan , Bonavida Benjamin
Published: May 2024

Tumor-associated macrophages (TAMs) are the predominant cell infiltrate in the immunosuppressive tumor microenvironment (TME). TAMs are central to fostering pro-inflammatory conditions, tumor growth, metastasis, and inhibiting therapy responses. Many cancer patients are innately refractory to chemotherapy and or develop resistance following initial treatments. There is a clinical correlation between the level of TAMs in the TME and chemoresistance. Hence, the pivotal role of TAMs in contributing to chemoresistance has garnered significant attention toward targeting TAMs to reverse this resistance. A prerequisite for such an approach requires a thorough understanding of the various underlying mechanisms by which TAMs inhibit response to chemotherapeutic drugs. Such mechanisms include enhancing drug efflux, regulating drug metabolism and detoxification, supporting cancer stem cell (CSCs) resistance, promoting epithelial-mesenchymal transition (EMT), inhibiting drug penetration and its metabolism, stimulating angiogenesis, impacting inhibitory STAT3/NF-κB survival pathways, and releasing specific inhibitory cytokines including TGF-β and IL-10. Accordingly, several strategies have been developed to overcome TAM-modulated chemoresistance. These include novel therapies that aim to deplete TAMs, repolarize them toward the anti-tumor M1-like phenotype, or block recruitment of monocytes into the TME. Current results from TAM-targeted treatments have been unimpressive; however, the use of TAM-targeted therapies in combination appears promising These include targeting TAMs with radiotherapy, chemotherapy, chemokine receptor inhibitors, immunotherapy, and loaded nanoparticles. The clinical limitations of these strategies are discussed.
Sağlık Çalışanlarına Şiddet Uygulama Niyetinin Değerlendirilmesi: Sultanbeyli Örneği
Authors: Şafak Tuğçe , Kızılkaya Yunus , Aslan Yasemin
Published: May 2024

Sağlık çalışanlarına yönelik şiddet yaygın toplumsal sorunlardan biridir. Bu çalışmanın amacı bireylerin sağlık çalışanlarına şiddet uygulama niyetinin değerlendirilmesi ve şiddete yönelik algılarının belirlenmesidir. Veriler, İstanbul ili Sultanbeyli ilçesi evreninde 550 kişiden, “Sağlık Çalışanlarına Şiddet Uygulama Niyeti Ölçeği” ve kişisel bilgi formu kullanılarak, anket tekniğiyle toplanmıştır. Verilerin analizinde The Number Cruncher Statistical System 2020 Statistical Software Programı kullanılmıştır. Geçmişte sağlık çalışanlarına şiddet uyguladığını belirten katılımcıların oranı %14.4 bulunmuştur. Her on katılımcıdan biri sağlık çalışanlarının şiddeti hak ettiğini düşünmektedir. İlk beş şiddet nedeninin; muayene için bekleme süresinin uzun olması (%36.7), hakarete uğrama (%16.5), muayene için ayrılan sürenin yetersiz olması (%16.5), yeterince bilgilendirilmeme (%11.4), sağlık çalışanlarının güler yüzlü ve kibar olmaması (%11.4) olduğu tespit edilmiştir. Çalışma bulguları 31-40 yaş aralığındaki bireylerin, eğitim seviyesi düşük olanların ve çalışan katılımcıların şiddet uygulama niyetinin daha yüksek olduğunu göstermektedir. Sağlıkta şiddetin önlenmesi politika yapıcıların, sağlık hizmeti sunucularının, toplumsal aktörlerin ve bireylerin iş birliğine dayalı yaklaşımını gerektirir.
An Interview with Dr. Kimber Thomas, Senior Innovation Specialist with the Connecting Communities Digital Initiative (CCDI) at the Library of Congress
Authors: Taylor Tucker , Thomas Kimber
Published: May 2024

In this interview, Dr. Thomas discusses the relationship between race and copyright. This conversation with Tucker Taylor of the Journal of Copyright in Education and Librarianship covers Dr. Thomas’s path to copyright education as well as her work at Connecting Communities Digital Initiative (CCDI) at the Library of Congress.  Dr. Thomas details the interaction of race and copyright, and how it relates to librarianship and education.  She also details ideas for librarians and educators to help improve our current situation.
How are pediatricians treating molluscum contagiosum? results from in-depth interviews
Authors: Martina Cartwright , Tomoko Maeda-Chubachi , Carolyn Enloe , Stephen Stripling , Adelaide Hebert
Published: May 2024

Molluscum contagiosum is a common, often persistent viral skin infection affecting children, and spread by infected individuals or objects. No molluscum management guidelines exist and only two FDA treatments have been approved in the last year, thus placing pediatricians in a clinical conundrum if molluscum treatment is warranted. When MC is particularly bothersome or persistent, parental anxiety may influence treatment. In-depth interviews with 25 pediatricians provided insight into the current MC treatment landscape and drivers of therapeutic intervention. This study reveals the important influence parental anxiety, patient characteristics, and parental involvement have on pediatrician’s molluscum management and treatment intervention practices. Professional society endorsed molluscum management guidelines and the use of safe and efficacious FDA-approved therapies would fill therapeutic gaps.
Labeling of microtubules using mouse anti-β-tubulin primary monoclonal antibody with secondary Fe-TAML-peg4-Cy5-goat anti-mouse IgG conjugate and oxidation of DAB with H2O2 for light and transmission electron microscopy v1
Authors: Adams Steven , R. Mackey Mason , Ellisman Mark
Published: May 2024

This protocol details the labeling of microtubules using mouse anti-β-tubulin primary monoclonal antibody with secondary Fe-TAML-peg4-Cy5-goat anti-mouse IgG conjugate and oxidation of DAB with H2O2 for light and transmission electron microscopy.
Setting up Zotero and Google Drive for syncing reference libraries (PC) v2
Authors: M. Frantz Carie
Published: May 2024

This protocol is useful if you wish to sync a LARGE reference library across multiple computers. It uses Zotero, which has built-in free syncing for up to 300 MB of files (roughly 50-100 papers). For larger libraries, it can make sense to use other cloud storage services to store and sync files. This protocol uses Google Drive, Windows PC, and Google Chrome. Zotero is free, open-source, powerful, and has an active user forum. I made this protocol for internal lab use (and my own reference), but welcome feedback if it's used by others!
Fetal and neonatal outcomes in syphilis infected pregnant women in Reunion Island v1
Authors: CRAMEZ Camille , Lien TRAN Phuong
Published: May 2024

Abstract Objectives : To evaluate the fetal and neonatal morbidity and mortality in pregnant women with syphilis during their pregnancy in Reunion Island, population benefiting from early and well-conducted screening and treatment. Methods : This is a retrospective observational study conducted in the four Reunion hospital centers between 2017 and 2022. The included patients were all pregnant patients with a biologically proven syphilitic infection and having given birth in one of the four centers mentioned. Results : 108 patients were included, with 113 fetuses and newborns. Fetal mortality rate was 2.7%, neonatal mortality rate was 1.8% and congenital syphilis rate was 7%. Despite 37% of patients with a vulnerable psycho-social context, 72% were screened early, 13% in the second trimester, 44% had received optimal treatment and 48% optimal syphilis monitoring. We found 43% of obstetric complications with 12% of IUGR and 4.6% of pre-eclampsia. 21% of newborns were preterm and 17% small for gestational age. There was a significant trend between psycho-social vulnerability and congenital syphilis. Conclusions : Fetal and neonatal morbidity and mortality is low in our Reunion Island population where screening andtreatment of syphilis are early and well conducted, although a vulnerable group of patients remain who require specific antenatal care.
A large scale bacterial attraction assay: a new quantitative bacterial migration assay suitable for genetic screens v1
Authors: Quiroz Monnens Thomas
Published: May 2024

Bacteria use various motility mechanisms to explore their environments. Chemotaxis is the ability of a motile bacterial cell to direct its movement in response to chemical gradients. A number of methods have been developed and widely used to study chemotactic responses to chemoeffectors including capillary, agar plug, microscopic slide, and microfluidic assays. While valuable, these assays are primarily designed to monitor rapid chemotactic responses to chemoeffectors on a small scale, which poses challenges in collecting large quantities of attracted bacteria. Consequently, these setups are not ideal for experiments like forward genetic screens. To overcome this limitation, we developed the Large Scale Bacterial Attraction assay (LSBA), which relies on the use of a Nalgene™ Reusable Filter Unit and other materials commonly found in laboratories. We validate the LSBA by investigating chemoeffector kinetics in the setup and by using chemoattractants to quantify the chemotactic response of wild-type, and motility impaired strains of the plant pathogenic bacterium Xanthomonas campestris pv. campestris and the environmental bacterium Shewanella oneidensis. We show that the LSBA establishes a long lasting chemoeffector gradient, that the setup can be used to quantify bacterial migration over time and that the LSBA offers the possibility to collect high numbers of attracted bacteria, making it suitable for genetic screens.
How to extract cyanobacterial genome v1
Authors: Ken Okumura Leonardo
Published: May 2024

This protocol describes how to extract cyanobacterial genomes. The main treatments are carried out by sodium iodide, lysozyme, proteinase K and SDS.