bluuebunny/crossref_metadata_2025 huggingface dataset:
Structure of theDietziaMrp complex reveals molecular mechanism of this giant bacterial sodium proton pump
Authors: Li Bin , Zhang Kaiduan , Nie Yong , Wang Xianping , Zhao Yan , Zhang Xuejun C. , Wu Xiao-Lei
Published: November 2020

Significance

Multiple resistance and pH adaptation (Mrp) complexes are the most sophisticated known cation/proton exchangers and are essential for the survival of a vast variety of alkaliphilic and/or halophilic microorganisms. Moreover, this family of antiporters represents the ancestor of cation pumps in nearly all known redox-driven transporter complexes, including the complex I of the respiratory chain. For the Mrp complex, an experimental structure is lacking. We now report the structure of Mrp complex at 3.0-Å resolution solved using the single-particle cryo-EM method. The structure-inspired functional study of Mrp provides detailed information for further biophysical and biochemical investigation of the intriguingly pumping mechanism and physiological functions of this complex, as well as for exploring its potential as a therapeutic drug target.
β-Cyclodextrin modified Pt(II) metallacycle-based supramolecular hyperbranched polymer assemblies for DOX delivery to liver cancer cells
Authors: Chen Wenzhuo , Li Xuefeng , Liu Chengfei , He Jia , Qi Miao , Sun Yue , Shi Bingbing , Sepehrpour Hajar , Li Hui , Tian Wei , Stang Peter J.
Published: November 2020

Significance

The development of chemotherapeutic drugs for cancer treatment has been limited by problems such as adverse side effects and inefficient performance of the drugs. To deliver multiple anticancer drugs for accurate and synergistic liver cancer treatment, a Pt(II) metallacycle-based drug delivery system, with endogenous redox and nitric oxide stimuli responsiveness and two chemotherapeutic agents is designed via the orthogonal self-assembly strategy. In vitro studies demonstrate that this system has good ability to deliver hydrophobic anticancer drug doxorubicin to HepG2 liver cancer cells. It also efficiently inhibits tumor cell growth for enhanced liver cancer treatment. This study provides an opportunity for the further design of metallacycle-based drug delivery systems for cancer therapy.
An empirical evaluation of Chinese college admissions reforms through a natural experiment
Authors: Chen Yan , Jiang Ming , Kesten Onur
Published: November 2020

Significance

While college admission decisions impact the educational experiences and labor market outcomes for millions of students each year, the best method to determine admissions has been vigorously debated by academics and policymakers across the globe. Driving this debate are a number of major theoretical and practical innovations over the past 2 decades. Using a natural experiment from China, we evaluate the performance of the immediate acceptance mechanism, used for many years for college admissions in China and school choice in the United States, and the newer parallel mechanism. We find that when provinces move to the parallel mechanism, students apply to more colleges and list more prestigious colleges first. We further find that the student–college matchings become more stable.
Ancient helium and tungsten isotopic signatures preserved in mantle domains least modified by crustal recycling
Authors: Jackson Matthew G. , Blichert-Toft Janne , Halldórsson Saemundur A. , Mundl-Petermeier Andrea , Bizimis Michael , Kurz Mark D. , Price Allison A. , Harðardóttir Sunna , Willhite Lori N. , Breddam Kresten , Becker Thorsten W. , Fischer Rebecca A.
Published: November 2020

Significance

The recent discovery of anomalous
182
W signatures in modern, plume-derived hotspot lavas provides evidence for survival of domains in Earth’s interior since the early Hadean. Only lavas with ancient, high-
3
He/
4
He signatures preserve anomalous
182
W signatures. However, it is not known why only high-
3
He/
4
He lavas have anomalous
182
W, while other hotspot lavas do not. We show that only hotspot lavas lacking strong recycled crust signatures exhibit high
3
He/
4
He and anomalous
182
W. This observation is explained by subduction of W- and
4
He-rich crust that has low
3
He/
4
He and normal
182
W: Following subduction, the crust masks high-
3
He/
4
He and anomalous
182
W signatures characteristic of ancient mantle domains. Our model links destruction of Hadean geochemical signatures with the geodynamic process of plate subduction and recycling.
Mycena
genomes resolve the evolution of fungal bioluminescence
Authors: Ke Huei-Mien , Lee Hsin-Han , Lin Chan-Yi Ivy , Liu Yu-Ching , Lu Min R. , Hsieh Jo-Wei Allison , Chang Chiung-Chih , Wu Pei-Hsuan , Lu Meiyeh Jade , Li Jeng-Yi , Shang Gaus , Lu Rita Jui-Hsien , Nagy László G. , Chen Pao-Yang , Kao Hsiao-Wei , Tsai Isheng Jason
Published: November 2020

Significance

We present the genomes of five new bonnet mushroom
Mycena
species, formerly the last fungal bioluminescent lineage lacking reference genomes. These genome-scale datasets allowed us to construct an evolutionary model pinpointing all possible changes in the luciferase cluster across all fungi and additional genes involved in bioluminescence. We show that luciferase clusters were differentially lost in different fungal lineages and in particular a substantial loss was observed in the mycenoid lineage. This can be attributed to genome regions of
Mycena
that underwent different evolutionary dynamics. Our findings offer insights into the evolution of how a gene cluster emerged 160 million years ago and was frequently lost or maintained due to differences in genome plasticity.
Designer broad-spectrum polyimidazolium antibiotics
Authors: Zhong Wenbin , Shi Zhenyu , Mahadevegowda Surendra H. , Liu Bo , Zhang Kaixi , Koh Chong Hui , Ruan Lin , Chen Yahua , Zeden Merve S. , Pee Carmen J. E. , Marimuthu Kalisvar , De Partha Pratim , Ng Oon Tek , Zhu Yabin , Chi Yonggui Robin , Hammond Paula T. , Yang Liang , Gan Yunn-Hwen , Pethe Kevin , Greenberg E. Peter , Gründling Angelika , Chan-Park Mary B.
Published: November 2020

Significance

We designed and synthesized antimicrobial compounds effective in killing pan-drug-resistant bacterial pathogens. These compounds have a large therapeutic window due to their low mammalian cell toxicity. A lead compound showed efficacy in treating murine model
S. aureus
and
P. aeruginosa
infections. In the case of the obligate respiratory bacterium
P. aeruginosa
we were unable to evolve strains resistant to these compounds. This paper points toward the potential of this type of compound to deal with the current threat of pan-resistant pathogens.
Unbiased optical mapping of telomere-integrated endogenous human herpesvirus 6
Authors: Wight Darren J. , Aimola Giulia , Aswad Amr , Jill Lai Chi-Yu , Bahamon Christian , Hong Karl , Hill Joshua A. , Kaufer Benedikt B.
Published: November 2020

Significance

Low-complexity and repetitive elements are difficult to study using existing sequencing technologies. Determination of the boundaries and structures of the termini of inherited chromosomally integrated HHV-6 (iciHHV-6) genomes is particularly challenging, as it integrates into highly repetitive human telomeres. We therefore developed a genome imaging approach that revealed the chromosomal location of the virus, its orientation, the presence of long internal telomeres at the host−virus junction, and the lengths of the distal telomeres capping the integrated virus genome. This genome imaging approach has wide applications for mapping transposable elements or large viruses that integrate into low-complexity regions of host genomes.
Capillary leakage provides nutrients and antioxidants for rapid pneumococcal proliferation in influenza-infected lower airways
Authors: Sender Vicky , Hentrich Karina , Pathak Anuj , Tan Qian Ler Alicia , Embaie Bethel Tesfai , Lundström Susanna L. , Gaetani Massimiliano , Bergstrand Jan , Nakamoto Rei , Sham Lok-To , Widengren Jerker , Normark Staffan , Henriques-Normark Birgitta
Published: November 2020

Significance

Mechanisms for why influenza A virus (IAV) infections sensitize for pneumococcal infections are not clear. Here, we show that IAV-induced capillary leakage results in influx of nutrients and antioxidants to the lungs, thereby promoting pneumococcal growth in the lower respiratory tract. The evoked inflammation leads to redox imbalances that require bacterial adaptation to the oxidized environment, including induction of the pneumococcal chaperone/protease HtrA that protects the bacteria from clearance by the immune system. The results give us insight into the delicate interplay between the bacteria and the host environment during coinfections that needs to be explored in order to find novel therapeutic approaches.
Reversal of autoimmunity by mixed chimerism enables reactivation of β cells and transdifferentiation of α cells in diabetic NOD mice
Authors: Tang Shanshan , Zhang Mingfeng , Zeng Samuel , Huang Yaxun , Qin Melissa , Nasri Ubaydah , Santamaria Pere , Riggs Arthur D. , Jin Liang , Zeng Defu
Published: November 2020

Significance

Cure of autoimmune type 1 diabetes (T1D) requires both reversal of autoimmunity and regeneration or resupply of insulin-producing β cells. We have observed that combination therapy with induction of haploidentical mixed chimerism and administration of gastrin and epidermal growth factor (EGF) cures firmly established T1D. The predominant source of β cell regeneration in mice comes from reactivation of dysfunctional insulin
lo
β cells and transdifferentiation of α cells. These studies have provided insights into β cell regeneration mechanisms in firmly established autoimmune T1D, in particular, reactivation of the insulin
lo
β cells after reversal of autoimmunity by induction of haploidentical mixed chimerism. These studies also provide a preclinical scientific basis for the feasibility of cure of long-standing T1D in humans.
Seasonal drought events in tropical East Asia over the last 60,000 y
Authors: Zhang Jianping , Lu Houyuan , Jia Jiwei , Shen Caiming , Wang Shuyun , Chu Guoqiang , Wang Luo , Cui Anning , Liu Jiaqi , Wu Naiqin , Li Fengjiang
Published: November 2020

Significance

The characters and mechanisms of seasonal precipitation changes during the last glaciation in tropical East Asia are enduring and important issues of debate for researchers from many disciplines. Sensitive indicators for annual/seasonal hydrological changes are keys to understanding the issues. Here, we use successive phytolith and pollen records to reconstruct the changes of precipitation in the catchment of the Huguangyan Maar Lake, an important site at northern tropical East Asia that could simultaneously receive climate signals from higher-latitude continent and tropical ocean. We then identify seven seasonal drought events during interstadials. The results indicate that both zonal and meridional circulations control seasonal hydrologic changes, which is of great significance to understand tropical atmospheric–ocean interactions under global changes.
Design of a multivalent bifunctional chelator for diagnostic
64
Cu PET imaging in Alzheimer’s disease
Authors: Cho Hong-Jun , Huynh Truc T. , Rogers Buck E. , Mirica Liviu M.
Published: November 2020

Significance

Metal radiotracers exhibit beneficial properties that can improve in vivo positron emission tomography (PET) imaging over traditional radionuclides, such as longer half-lives, facile last-stage radiolabeling steps, and the potential for dual imaging-therapy applications. Among such radionuclides,
64
Cu exhibits a longer half-life (t
1/2
= 12.7 h) that allows for later PET imaging times and the ability to distribute
64
Cu imaging agents to facilities that do not have an on-site cyclotron. Herein, we report a
64
Cu PET imaging agent that shows appreciable in vivo brain uptake and exhibits high specific affinity for beta-amyloid aggregates, leading to the successful PET imaging of amyloid plaques in the brains of 5xFAD mice versus those of wild-type mice.
High-resolution mouse subventricular zone stem-cell niche transcriptome reveals features of lineage, anatomy, and aging
Authors: Xie Xuanhua P. , Laks Dan R. , Sun Daochun , Poran Asaf , Laughney Ashley M. , Wang Zilai , Sam Jessica , Belenguer German , Fariñas Isabel , Elemento Olivier , Zhou Xiuping , Parada Luis F.
Published: November 2020

Significance

Adult neural stem cells (NSC) are closely related to multiple neurological disorders and brain tumors. Comprehensive investigation of their composition, lineage, and aging will provide insights that may lead to enhanced patient treatment. This study applies a transgene to label and manipulate neural stem/progenitor cells and monitor their evolution during aging. Together with high-throughput single-cell RNA sequencing, we are able to analyze the subventricular zone cells from infancy to advanced age with unprecedented granularity. Diverse cell states are identified in the stem-cell niche, and an aging-related NSC deficiency in transition from quiescence to proliferation is identified. The related biological features provide rich resources to inspect adult NSC maintenance and neurogenesis.
Datura
quids at Pinwheel Cave, California, provide unambiguous confirmation of the ingestion of hallucinogens at a rock art site
Authors: Robinson David W. , Brown Kelly , McMenemy Moira , Dennany Lynn , Baker Matthew J. , Allan Pamela , Cartwright Caroline , Bernard Julienne , Sturt Fraser , Kotoula Elena , Jazwa Christopher , Gill Kristina M. , Randolph-Quinney Patrick , Ash Thomas , Bedford Clare , Gandy Devlin , Armstrong Matthew , Miles James , Haviland David
Published: November 2020

Significance

Proponents of the altered states of consciousness (ASC) model have argued that hallucinogens have influenced the prehistoric making of images in caves and rock shelters. However, the lack of direct evidence for the consumption of hallucinogens at any global rock art site has undermined the ASC model. We present the first clear evidence for the ingestion of hallucinogens at a rock art site, in this case, from Pinwheel Cave, California. Quids in the cave ceiling are shown to be
Datura wrightii
, a Native Californian entheogen, indicating that, rather than illustrating visual phenomena caused by the
Datura
, the rock paintings instead likely represent the plant and its pollinator, calling into question long-held assumptions about rock art and the ASC model.
Moth wings are acoustic metamaterials
Authors: Neil Thomas R. , Shen Zhiyuan , Robert Daniel , Drinkwater Bruce W. , Holderied Marc W.
Published: November 2020

Significance

Bats and moths are embroiled in an evolutionary arms race. Using ultrasonic biosonar, bats detect their insect prey, which in turn deploy diverse strategies to avoid predation. Here, we show that some moth species evolved wings covered with a canopy of scales that reduces ultrasonic echoes. Our empirical and mathematical analysis together show that moth wings exhibit key features of a desirable technological acoustic metamaterial. This work enriches our understanding of the structural and functional complexity of lepidopteran wings and reveals enticing new ways to design, using bioinspired metamaterial properties, high-performance acoustic panels and noise mitigation devices.
The SERCA residue Glu340 mediates interdomain communication that guides Ca
2+
transport
Authors: Geurts Maxwell M. G. , Clausen Johannes D. , Arnou Bertrand , Montigny Cédric , Lenoir Guillaume , Corey Robin A. , Jaxel Christine , Møller Jesper V. , Nissen Poul , Andersen Jens Peter , le Maire Marc , Bublitz Maike
Published: November 2020

Significance

We present a crystal structure, functional data, and molecular dynamics (MD) simulations of the sarco(endo)plasmic reticulum Ca
2+
-ATPase (SERCA) mutant E340A. The mutation slows Ca
2+
-binding kinetics, and the structural differences between wild type and E340A indicate that the mutation disrupts a central interdomain “communication hub” governing Ca
2+
binding/dissociation. MD simulations reveal altered dynamics in regions mediating Ca
2+
occlusion, a critical step in SERCA’s alternating access mechanism. The mutation stabilizes a more occluded state of the Ca
2+
sites. The strict conservation of Glu340 among P-type ATPases is the result of its critical role in interdomain communication between the cytosolic headpiece and the transmembrane domain, ensuring a delicate balance between dynamics of ion binding, occlusion, and release—key steps in the transport process.
A peptide of a type I toxin−antitoxin system induces
Helicobacter pylori
morphological transformation from spiral shape to coccoids
Authors: El Mortaji Lamya , Tejada-Arranz Alejandro , Rifflet Aline , Boneca Ivo G. , Pehau-Arnaudet Gérard , Radicella J. Pablo , Marsin Stéphanie , De Reuse Hilde
Published: November 2020

Significance

Helicobacter pylori
, a gastric pathogen causing 800,000 deaths in the world annually, is encountered both in vitro and in patients as spiral-shaped bacteria and as round cells named coccoids. We discovered that the toxin from a chromosomal type I toxin−antitoxin system is targeting
H. pylori
membrane and acting as an effector of
H. pylori
morphological conversion to coccoids. We show that these round cells maintain their membrane integrity and metabolism, strongly suggesting that they are viable dormant bacteria. Oxidative stress is identified as a signal inducing toxin expression and coccoid formation. Our findings reveal insights into a form of dormancy of this bacterium that might be associated with
H. pylori
infections refractory to treatment.
Synaptotagmin-7 deficiency induces mania-like behavioral abnormalities through attenuating GluN2B activity
Authors: Wang Qiu-Wen , Lu Si-Yao , Liu Yao-Nan , Chen Yun , Wei Hui , Shen Wei , Chen Yan-Fen , Fu Chong-Lei , Wang Ying-Han , Dai Anbang , Huang Xuan , Gage Fred H. , Xu Qi , Yao Jun
Published: November 2020

Significance

Although synaptotagmin-7 (Syt7) has been found to play an important role in bipolar-like behavioral abnormalities in mice, the molecular mechanisms underlying the aberrant behaviors remain unknown. The reason for the promising efficacy of
N
-methyl-
d
-aspartate receptor (NMDAR) antagonists, such as ketamine, in the treatment of bipolar depression is also unclear. In the present study, we demonstrated that in human induced pluripotent stem cell-derived and mouse hippocampal neurons, Syt7 triggered glutamate release in the peripheral active zone, which efficiently activated juxtaposed postsynaptic GluN2B-NMDARs. As a result of the Syt7 deficits, GluN2B-NMDARs became hypoactive and consequently generated antidepressant effects, which induced mania-like behavioral abnormalities in the animals.
Structure–function subsystem models of female and male forebrain networks integrating cognition, affect, behavior, and bodily functions
Authors: Swanson Larry W. , Hahn Joel D. , Sporns Olaf
Published: November 2020

Significance

The forebrain is required for generating mammalian voluntary and innate behaviors. Multiresolution consensus cluster analysis generated a panoramic, hierarchical view of rat intraforebrain structure–function subsystem organization, from the top level with just two relatively independent mirror image subsystems centered on either side of the brain, to over 90 bottom-level subsystems. Each top-level subsystem has two interconnected subsystems: one includes the medial forebrain bundle and integrates instinctive survival behaviors with relevant physiological responses and affect, whereas the other uses the lateral forebrain bundle and mediates voluntary behavior control, with associated cognition. The intraforebrain network is sexually dimorphic, and targeted computational lesions of nodes implicated in human Alzheimer’s disease and depression produce severe, differential, global network changes in males and females.
Environmental noise degrades hippocampus-related learning and memory
Authors: Zhang Yifan , Zhu Min , Sun Yutian , Tang Binliang , Zhang Guimin , An Pengying , Cheng Yuan , Shan Ye , Merzenich Michael M. , Zhou Xiaoming
Published: November 2020

Significance

The noise pollution accompanying industrialization is a risk factor to human health. Here, we show in a rodent model that even moderate-level noise at ∼65 dB SPL that has little effect on stress status can substantially impair hippocampus-related learning and memory by altering the plasticity of synaptic transmission. It is possible that because moderately loud noise does not affect peripheral hearing per se, the negative impacts of chronic exposure to such noise are currently not well characterized. Our results indicate the importance of more thoroughly defining these possibly hitherto unappreciated hazards of noise pollution in modern human environments.
Some structural similarities in the outcomes of language contact with
Russian
Authors: Forker Diana , Grenoble Lenore A.
Published: November 2020

This paper surveys a broad range of languages in contact
with Russian to provide an overview of a core set of similarities in the
outcomes of contact-induced change. We consider both lexical and structural
borrowings, focusing on five categories: adjectives, verbs, indefinite
pronouns, participles and conjunctions, and the structure of complex
clauses. There are clear genetic and areal tendencies for particular
strategies for the integration of loan verbs, while we also find broad
similarities in the changes across all languages surveyed, despite their
differences typologically and genetically, and also in terms of their
demographics and local contact ecologies.
Analyzing Modern Chinese Pidgin Russian
Authors: Perekhvalskaya Elena
Published: November 2020

The article is devoted to the analysis of specific
varieties of Russian used by older Udihe and Nanai in the Primorsky and Khabarovsky territories. These codes are characterized by a high level of
variability. It is shown that these are contemporary varieties of Chinese
Pidgin Russian (ChPR), which was a means of communication between Russians
and the local indigenous population before the 1960s. Invariable forms similar
to pidgin are in free variation with inflected Russian forms. I argue that a
stabilized pidgin or a creole may show no supervariability, with Tok-Pisin
being an example. Stabilized Pidgins and Creoles are no more variable than
languages with an ordinary history of formation. Thus, Chinese Pidgin
Russian shows significant stability: different sources display similar or
very close variants (for details, see Perekhvalskaya, 2008, 2013). This paper is a case study analysis of variability in
modern forms of Chinese Pidgin Russian spoken in the Ussuri region of
Russia: it presents linguistic processes occurring lately in the Chinese
Pidgin Russian. In the course of its history, this variety underwent a stage
of a relative stability followed by a more variable post-pidgin stage. The
latter will be thoroughly analyzed in this paper. The Ussuri region is the
territory annexed by Russia under the Aygun peace treaty in 1858–1860. It is
bounded by the Amur river in the North, by the Ussuri and Vaku (after 1972
Malinovka) rivers in the West, and by the Sea of Japan in the East.
Language data and maps
Authors: Koryakov Yuri
Published: November 2020

The maps and data in this chapter give an overall picture of the demographics and social status of the languages discussed in the present volume. In the 1st table, each language mentioned in the book is provided with basic genealogical, sociolinguistic and demographic data. The more complicated cases are detailed in the followed sections. Three maps show the distribution of the same languages and their ranking according to the following parameters: (1) number of speakers; (2) genealogical classification; (3) levels of endangerment and official status.
Instability of regularized 4D charged Einstein-Gauss-Bonnet de-Sitter black holes *
Authors: Liu Peng , Niu Chao , Zhang Cheng-Yong
Published: November 2020

We studied the instability of regularized 4D charged Einstein-Gauss-Bonnet de-Sitter black holes under charged scalar perturbations. The unstable modes satisfy the superradiant condition, but not all of the modes satisfying the superradiant condition are unstable. The instability occurs when the cosmological constant is small and the black hole charge is not too large. The Gauss-Bonnet coupling constant further destabilizes black holes when both the black hole charge and the cosmological constant are small and further stabilizes black holes when the black hole charge is large.
Expert System in Clustering the Damage of a Motorcycle Matic with the K-Means Algorithm
Authors: Rusdiansyah  , Badrul Mohammad , Tuslaela  , Supendar Hendra , Suharyanti Nining , Junaidi Agus
Published: November 2020

Research on Expert Systems to Diagnose Matic Motorcycle Engine Damage by applying Algortima K-means. Research to detect damage to an automatic motorcycle by observing the symptoms of an automatic motorcycle. This study aims to help Matic Motorcycle users to find out about damage to the automatic motorcycle based on the results of the application of the K-Means algorithm without having to meet with experts directly. Research by applying Algortima k-Means by forming groupings based on electrical damage, compression and engine performance, continuous variable transmission timing and automatic sensors so that the damage can be grouped. The results of the expert system research with the K-Means Algorithm can help matic motorcycle users to find out the type of damage based on the grouping that has been determined by the K-Means algorithm.
Classification System Of Toddler Nutrition Status using Naïve Bayes Classifier Based on Z- Score Value and Anthropometry Index
Authors: Putri Tiara Eka , Subagio Ridho Taufiq , Kusnadi  , Sobiki Petrus
Published: November 2020

The system of nutritional status assessment for a toddler is crucial to monitor the growth of a toddler. This present study was carried out to build a classification system for determining the assessment of toddler nutritional status using naive bayes classifier based on value the z-score and index of Anthropometry. The data was used to perform classification include gender, age, height and weight. The data was calculated using the z-score to get nutritional status based on anthropometric indices-weight-for-age, height-for-age, weight-for-height for classified use Naive Bayes Classifier. This study used 225 data of toddlers. Testing system used 55 data as training and 175 data as testing with 100% accuracy. The results of this study was a system that could be used to perform classification of nutritional status based on a combination of three anthropometric indices using the Naive Bayes Classifier. Naive Bayes Classifier performed classification interpretation of nutritional toddler consisting of malnutrition, normal and over-nutrition. This study showed that the classification of nutritional status was on 175 data generating the highest percentage was malnutrition of 44.58%.
Identification of Grape Leaf Diseases Using Convolutional Neural Network
Authors: Hasan Moh. Arie , Riana Dwiza , Swasono Sigit , Priyatna Ade , Pudjiarti Eni , Prahartiwi Lusa Indah
Published: November 2020

The presence of leaf diseases in grapes can reduce the productivity of grapes and result in losses for farmers. Leaf diseases are mainly caused by bacteria, fungi, virus etc. A proper diagnosis of disease in plants is needed in order to take appropriate control measures. This paper aims to assist in the identification and classification of grape leaf diseases Convolutional Neural Network (CNN). CNN is basically an artificial neural network architecture that requires repeated training processes to get good accuracy. CNN consists of 3 stages, namely Data Input, Feature Learning, and Classification. The implementation of CNN in this study uses Keras libraries that use the python programming language. Keras is a framework created to facilitate learning of computers. The CNN training process using 0.0001 learning rate obtained results with an accuracy rate of 91,37%
The Determination of Yarn Supplier by Using the Weight Product Method
Authors: Indriyani Fintri , Irfiani Eni , Schaduw Frans Edward , Anwar Syaiful , Hidayat Rahmat
Published: November 2020

The Upstream Supply Chain Management is the initial component of the supply chain where the company gets raw materials from suppliers for the production process. This process is very important in the sustainability of a company’s production. The obstacle experienced at this stage is how the company decides which suppliers to choose from the many suppliers who want to offer their products. Also, it is experienced by PT. Mayer Indah Indonesia, which currently has 14 prospective suppliers, each of which has a different weight based on the assessment criteria of the company. The purpose of this study is to assist companies in making decisions to determine the best suppliers by using the Weight Product (WP) method, while the selection criteria are based on price, quality and the delivery accuracy. The results of the study can be implemented by the company to determine the right supplier in accordance with the criteria that have been required.
Design of IT Governance Evaluation Using COBIT Framework through Capability Maturity in Department of Transportation Cirebon
Authors: Magdalena Lena , Solihah Yuni Awalaturrohmah
Published: November 2020

IT Governance Evaluation has not been carried out optimally because supervision and evaluation of IT is only done if there are complaints from work units regarding IT services. These problems relate to the services that need to be provided to users of the information system, starting from the operations that need to be carried out on data security, sustainability aspects, training of human resources that support the processes of the information system and related to the support processes that should first be determined to be able to give service. This research method used qualitative research by using COBIT 5 framework dealing with the system analysis and design using the Usecase Diagram, Activity Diagram, Sequence Diagram, and Class Diagram tools. The programming language used is PHP Hypertext Prepocessor (PHP) while the database uses MySQL. This application was created to provide information about managing the IT Governance Evaluation in Department of Transportation Cirebon. The results of this study is IT Governance Evaluation using Cobit 5 Framework. Regarding to the IT Governance using COBIT 5, the Capability Maturity Level is at 3.28 that means defined-process. It represents that the IT Governance has been implemented to reach the goal of this organization. This assessment was aimed to determine the capabilities of IT performance and make recommendations for the proper management of IT Performance Management so that it can be used as a guide that can be used by the user and can increase the use of facilities optimally for better improvement of Department of Transportation Cirebon.
Macroscopic Modelling of Pedestrian Flows Based on Conservation Law
Authors: Windyani Finna , Gunawan P. H. , Tarwidi Dede
Published: November 2020

Overcrowded of sidewalks can reduce the level of satisfaction of pedestrians. The large crowd on the sidewalks results in slowing down time of the pedestrian to arrive in their destination. This studt, focuses on pedestrian flows modelling using the macroscopic model. Numerical approximation of the macroscopic model is formulated as scalar hyperbolic conservation laws. The Lax-Wendroff scheme is used to discretize the equation of conservation laws. The simulation results show that the numerical approximation in term of density confirms the exact solution. In this simulation, the velocity function is obtained by curve fitting of observation data using linear regression method. The observation data, which are consist of velocity-density relation, are obtained from observation of pedestrian flows. The study case of this research conducts on the sidewalks of Braga Street, Bandung, Indonesia. There are two velocity functions used in the simulation, i.e. c
1 = 0.58206 + −0.94476ρ and c
2 = 0.59926 + −0.78052ρ, respectively. In performing the velocity function c
2, the pedestrian leader position is approximately 1 meter in front of the pedestrian leader using the velocity function c
1 at final time T = 20 seconds and T = 30 seconds. Overall, the numerical experiment shows that the pedestrian leader using the velocity functions c
2 is faster than by using c
1.
Implementation of Neural Network Method for Air Quality Forecasting in Jakarta Region
Authors: Kristiyanti Dinar Ajeng , Purwaningsih Esty , Nurelasari Ela , Kaafi Ahmad Al , Umam Akhmad Hairul
Published: November 2020

The quality of air can be influenced by the amount of pollution that occurs in an area. The city of Jakarta is ranked in the top ten as the nation’s capital with the worst air quality in the world. Poor air quality both inside and outside the room can have an impact on the emergence of various diseases and even death. For this reason, forecasting of air quality in the city of Jakarta, Indonesia is needed to anticipate the likely impact that will arise. In this study forecasting air quality using the neural network method in which this method has the advantage of being able to solve problems, especially large data samples and has been able to prove in handling non-linear problems. The data collection used is secondary data from the Environmental Service Office of DKI Jakarta Province as many as 2989 records with variables as determinants consisting of 5 of which PM10, SO2, CO, O3, NO2 and 1 output variable are good, moderate, unhealthy and very unhealthy. From the calculations result in this study it is known that the Neural Network method obtained an accuracy performance of 88.86% in which the Lubang Buaya area noted as the most unhealthy air quality.
Designing Face Recognition Teacher Wellbeing Application that Optimizes Teacher’s Quality Work Life
Authors: Rosyidi Unifah , Sasmoko  , Indrianti Yasinta , Manalu Sonya Rapinta , Lubis Ramot , Moniaga Jurike , Abu Bakar Abu Yazid Bin
Published: November 2020

Meeting the demands of industry 4.0, teachers are required to improve the quality of their profession. Quality professions will not be achieved if the teacher does not have a quality person and at the same time prosperous. By utilizing technological developments, the teacher can monitor and diagnose the condition of the self wellbeing on an ongoing basis, so as to optimize the quality of work life. Teacher’s Quality Work Life is very important for teachers themselves as educators, institutions and the nation and state. Quality work life can be a measure of teacher growth vertically and horizontally along with the growth of the institution. The encounter of Education, psychological trait with Artificial Intelligence is called Positive Education is the main objective of this research. One form is a self diagnostic that is able to diagnose psychological conditions to produce optimal quality. This research aims to create an application face recognition design through Neuroresearch and Waterfall research methods. The results of the study were designed to build the Face Recognition Teacher Wellbeing application.
Integrated Monitoring Platform for Collaborative Youth Communities in Indonesia: A Case of e-Government Implementation for the Rural Millennial
Authors: Renaldi F , Ramandhani M P , Djamal E C , Santikarama I
Published: November 2020

Electronic monitoring systems (e-Monitoring) is the term used for applying technology to a reporting system, used as a medium of supervising for various activities. One of the areas that are prone to chaotic problems thus needs monitoring, is the youth community. While youth empowerment promised high result for the society, it lacks control can also create all sorts of issues. Although there have been many studies dealing with the use of technology in youth communities, none have intensely discussed sharing information between each youth communities, especially those villages youth communities that connect through one sub-district youth community. We consider this as an essential issue since those youth communities are adjacent to one another; hence, a repetition of the program would be an ineffective use of budgets. Upon the implementation of the system, we conducted a user acceptance test. The test was made with 57 test scenarios for five types of users with an acceptance rate of 82,81%. Attaching this system into another e-Government system such as the municipal systems or the banking systems should be a promising future work to be done.
Design and Implementation of Rivest Shamir Adleman’s (RSA) Cryptography Algorithm in Text File Data Security
Authors: Sihotang Hengki Tamando , Efendi Syahril , Zamzami Elvyawati M , Mawengkang Herman
Published: November 2020

Security of data text file on a computer can be done by utilizing encryption and decryption techniques. One technique is encryption and decryption of data encryption system text file with cryptography. Cryptography is the science or art to randomize and secure the message to avoid the manipulation of data by performing encryption and decryption on the data of the text file. Cryptographic algorithm consists of several types of one example is the algorithm Rivest Shamir Adleman (RSA). The RSA algorithm is one of asymmetric cryptography technique ie riptografi modern technique that has two keys of the public key used for encryption and a private key techniques to make the process deskripsi. The purpose of this research is to design and implement RSA cryptography algorithm on text file data security. The results of this design is a dekstop based software. This research is expected to provide benefits to the users, individually as well for other organizations.
App Review Sentiment Analysis Shopee Application In Google Play Store Using Naive Bayes Algorithm
Authors: Pratmanto Dany , Rousyati Rousyati , Wati Fanny Fatma , Widodo Andrian Eko , Suleman Suleman , Wijianto Ragil
Published: November 2020

An online marketplace site is a shopping place that is currently popular with the community because it offers a variety of convenience and one of the marketplace apps is Shopee. Some people are satisfied with the service provided by the Shopee app. But unisex some people who give complaints about this application. User-provided response to Shopee app in the Comments field of Shopee Google Play Store can be analyzed for negative and positive sentiments. This research aims to assist Shopee’s management of the positive or negative opinions of application users and can provide empirical evidence for related theories so that it can be used as a donation of thought for the development of theories Next. With the number of reviews shown, you need an analysis that can classify these reviews into positive or negative classes. The method used for the sentiment analysis of Shopee app reviews is the Naive Bayes algorithm obtaining an accuracy yield of 96,667%
Employee attendance application using location based service (lbs) method based on android
Authors: Fatkharrofiqi Achmad , Kuswanto Herman , Rahman Taufik , Sumarna  , Handono Felix Wuryo , Nurdin Hafis
Published: November 2020

PT. Brainmatics Cipta Informatika is an IT Training and Consulting company located in Jakarta. The development of a company can not be separated from the role of employees in it. The success assessment of existing human resources apart from the aspect of success in the field of work is also inseparable from the assessment of the conditions including the assessment of attendance. Problems that occur at PT. Cipta Cipta Brainmatics is the process of existing employee attendance, attendance is done by using Odoo’s web based Enterprise Resource Planning system that stores employee data on coming and outgoing hours that can be accessed anywhere without any location or area restrictions, so employees can do attendance at anywhere, even without having to come to the office. Based on these problems, an Android-based attendance application was designed by utilizing the Location Based Service (LBS) feature that can be operated using a smartphone and can be used with a limited area determined for the attendance process. This application was built with the Java programming language using Android Studio, PostgreSQL and Objecbox software as its database. It is expected that the application that is designed can resolve the attendance issues that exist at PT. Brainmatics Cipta Informatika.
The Analysis of Digital Provider Sentiment Of ‘by.u’ On Google Play Which Uses The Support Vector Machine (SVM) Method
Authors: Ardiansyah Angga , Aji Sopian , Pratmanto Dany , Kuryanti Sandra Jamu , Putra Octa Pratama , Adiwihardja Cep
Published: November 2020

The development of technology in the industrial revolution 4.0 is very rapid in various sectors in trade, communication and other sectors; both nationally and internationally. One of the fields, it is the telecommunications sector. Becoming one of the digital based providers of ‘by.u’ from Telkomsel which is the first digital operator in Indonesia. The algorithm testing uses Support Vector Machine (SVM) from 300 user review data applications. The ‘by.u’ review consists of 150 negative reviews; which refers to dislike or bad expressions. Next, 150 positive reviews; which refers the constructive suggestion or support the text in using Indonesian. The resulting model gets the results of testing accuracy by using Support Vector Machine with accuracy value shows that: 87.24
Analysis of Community Satisfaction Level Against the Ministry of Health’s Infection Emerging Websites Using Webqual 4.0
Authors: Said Fadillah , Astoni Khabib , Riana Dwiza , Wahyuni Asri
Published: November 2020

As one form of communication and information media, web sites have a very big role in representing a government institution to interact with the public. A website is designed in such a way as to meet certain service quality standards set by the developer. However, good service quality must also consider perceptions from the point of view of users in this case the wider community. The study purpose is to determine the level of community satisfaction with the quality of the website as an indicator of success by the government in conveying information to the public. The method used in this study is to distribute questionnaires with a Webqual approach which is of three categories, specifically Usability Dimension, Information Quality Dimension, and Service Interaction Dimension. The data were obtained then analyzed using the Structural Equation Model (SEM) Technique with the SmartPLS 3 software. Based on a survey of 104 respondents, it was found that in general users were satisfied with the services on the website.
Villages Status Classification Analysis Involving K-Means Algorithm To Support Kementerian Desa Pembangunan Daerah Tertinggal dan Transmigrasi Work Programs
Authors: Hasugian Paska Marto , Hutahaean Harvei Desmon , Sinaga Bosker , Sriadhi  , Silaban Saranom
Published: November 2020

Data mining is a technique of extracting information that has not been known before in a collection of data in the database. Data mining has been applied in various fields that require extracting information, some of the work that can be generated with data mining is classification, prediction, and data grouping. In this study, an analysis of village data collection was carried out to explore the potential or knowledge of the data that has been presented with the main objective of producing a grouping of village status. To support clustering or grouping activities the K-means algorithm used with the general process is to carry out the modeling process without supervision and is one of the methods for grouping data with a system partition, with the principle of allocating each data to the centroid or the closest average, work steps conducted in support of this research is to collect data related to the analysis of data grouping and proceed with the calculation process in accordance with the work steps of the K-means algorithm, the amount of data used as a test of 303 villages scattered in the old Padang regency. The results of calculations by displaying a new group of Cluster 0 is occupied by 120 villages, cluster 1 with a total data of 123 villages, Cluster 2 with a total of 6 villages, cluster 3 with a total of 33 villages while cluster 4 with a total of 21.
Analysis of Factors Affecting Quality of corona.jatengprov.go.id Website Towards User Satisfaction using Webqual 4.0 Method
Authors: Nugraha Ranu Agastya , Andriyanto Dwi , Riana Dwiza , Khasanah Siti Nur
Published: November 2020

This research focuses on how to measure the quality of a website using the Webqual 4.0 method, what are the factors that influence user satisfaction. The PLS-SEM technique was used to verify questionnaire data from a sample of 155 respondents. The result shows that the quality of service interaction has a significant (positive) effect of 54.2% compared to the aspect of information quality and usability quality that has no significant effect. These results indicate that the corona.jatengprov.go.id site users expect quality of service interaction as a measure of satisfaction from the quality of a website. Users are more likely not to see deficiencies in the aspect of information quality and usability quality according to the assessment of satisfaction given. Therefore, the corona.jatengprov.go.id website manager needs to focus on the quality of service interaction aspects of website as a priority in improving grade.
Swietenia Mahagoni Wood Defects Segmentation Using YIQ Color Space and Thresholding
Authors: Rahayu Sri , Qhomariyah Nurul , Purnama Jajang Jaya , Riana Dwiza , Achyani Yuni Eka , Ariani Fattya
Published: November 2020

The biggest income from Southeast Asian countries came from timber production export activities. The potential for timber exports in Indonesia continued to increase every year. This skyrocketing potential needed to be improved by maintaining quality so that trust and good cooperation continued to be established. The quality of wood has closely related to wood defects, the faster detection of wood defects would be the faster also determines the quality of wood. Current technology has being developing rapidly to help productive human activities, image processing has being a breakthrough to be able to detect wood defects. This study aims to detect wood defects by segmenting Swietenia Mahagoni wood images by using the YIQ color space and Thresholding has resulted in a fairly good segmentation that is successful in segmenting the types of bark grown wood defects on bontos and defects in healthy knot on the body of wood with each percentage of 83.3%.
Assessing E-Commerce Success from a Millennial Perspective in Indonesia
Authors: Mahendra Irfan , Sulistianto SW , Mulyani Astriana , Wiyatno Agus , Rosanto Oki
Published: November 2020

This research was conducted to assess the success of e-commerce from the perspective of the millennial generation in Indonesia. This research was conducted through a case study on the Bukalapak Application, which in recent times has decreased from the perspective of the number of visitors. This study refers to the DeLon and McLean Updated Information System Success Model. The measurement indicators used in this study were modified based on a review of several recent studies. Data collection was carried out using a questionnaire distributed to 291 respondents consisting of Bukalapak Application users who are included in the millennial generation in Indonesia. Based on the research results above, it can be concluded that all hypotheses can be accepted. Where the quality of information and customer satisfaction has a stronger influence on the use of the Bukalapak Application. Whereas service quality and system quality have a stronger influence on customer satisfaction in using the Bukalapak Application. Then user satisfaction has a stronger effect than using the system at a net profit. It is also known that the millennial generation in Indonesia also considers that the Bukalapak Application has good information quality, system quality, and service quality. Millennial also claims to have used the Bukalapak Application and expressed satisfaction with using the Bukalapak Application and stated that the Bukalapak Application was useful.