bluuebunny/crossref_metadata_2025 huggingface dataset:
Combining Data from Multiple Sources to Evaluate Spatial Variations in the Economic Costs of PM2.5-Related Health Conditions in the Beijing–Tianjin–Hebei Region
Authors: Zhang Xiya , Hu Haibo
Published: October 2019

Fine particulate matter, known as PM2.5, is closely related to a range of adverse health outcomes and ultimately imposes a high economic cost on the society. While we know that the costs associated with PM2.5-related health outcomes are not uniform geographically, a few researchers have considered the geographical variations in these costs because of a lack of high-resolution data for PM2.5 and population density. Satellite remote sensing provides highly precise, high-resolution data about how PM2.5 and population density vary spatially, which can be used to support detailed health-related assessments. In this study, we used high-resolution PM2.5 concentration and population density based on remote sensing data to assess the effects of PM2.5 on human health and the related economic costs in the Beijing–Tianjin–Hebei (BTH) region in 2016 using exposure-response functions and the relationship between health and economic costs. The results showed that the PM2.5-related economic costs were unevenly distributed and as with the population density, the costs were mainly concentrated in urban areas. In 2016, the economic costs of PM2.5-related health endpoints amounted to 4.47% of the total gross domestic product in the BTH region. Of the health endpoints, the cost incurred by premature deaths accounted for more than 80% of the total economic costs associated with PM2.5. The results of this study provide new and detailed information that could be used to support the implementation of national and regional policies to reduce air pollution.
Is Carbon Dioxide (CO2) Emission an Important Factor Affecting Healthcare Expenditure? Evidence from China, 2005–2016
Authors: Chen Linhong , Zhuo Yue , Xu Zhiming , Xu Xiaocang , Gao Xin
Published: October 2019

As a result of China’s economic growth, air pollution, including carbon dioxide (CO2) emission, has caused serious health problems and accompanying heavy economic burdens on healthcare. Therefore, the effect of carbon dioxide emission on healthcare expenditure (HCE) has attracted the interest of many researchers, most of which have adopted traditional empirical methods, such as ordinary least squares (OLS) or quantile regression (QR), to analyze the issue. This paper, however, attempts to introduce Bayesian quantile regression (BQR) to discuss the relationship between carbon dioxide emission and HCE, based on the longitudinal data of 30 provinces in China (2005–2016). It was found that carbon dioxide emission is, indeed, an important factor affecting healthcare expenditure in China, although its influence is not as great as the income variable. It was also revealed that the effect of carbon dioxide emission on HCE at a higher quantile was much smaller, which indicates that most people are not paying sufficient attention to the correlation between air pollution and healthcare. This study also proves the applicability of Bayesian quantile regression and its ability to offer more valuable information, as compared to traditional empirical tools, thus expanding and deepening research capabilities on the topic.
Knowledge, Attitude, and Practice Associated with Antibiotic Use among University Students: A Survey in Nepal
Authors: Shah Poonam , Shrestha Rajeev , Mao Zongfu , Chen Yilin , Chen Yan , Koju Pramesh , Liu Xinliang , Li Hao
Published: October 2019

The purpose of this study was to conduct a preliminary study to assess knowledge, attitude, and practice (KAP) associated with antibiotic use among medical students (MS) and non-medical students (NMS) at Kathmandu University, Nepal. A self-administered questionnaire was distributed to 1223 students for a cross-sectional study. In total, 1222 questionnaires collected from 609 MS and 613 NMS were regarded as effective. A t-test and Chi-square test were applied to analyze the data. A total of 25 out of 39 questions in the KAP survey were found to have statistical significance. The MS showed higher levels of knowledge/attitude/practice associated with antibiotic use than the NMS. Significant gaps were found in and between the MS and NMS in the first and final years of study. Interventions, such as lectures, courses, workshops, and seminars on antibiotic use, along with internet and media campaigns, etc., are needed to improve the awareness and change the behavior of both the MS and the NMS of universities with regards to the rational use of antibiotics.
HDAC2 Regulates Glial Cell Activation in Ischemic Mouse Retina
Authors: Sung Mi Sun , Heo Hwan , Eom Gwang Hyeon , Kim So Young , Piao Helong , Guo Yue , Park Sang Woo
Published: October 2019

The current study was undertaken to investigate whether histone deacetylases (HDACs) can modulate the viability of retinal ganglion cells (RGCs) and the activity of glial cells in a mouse model of retinal ischemia-reperfusion (IR) injury. C57BL/6J mice were subjected to constant elevation of intraocular pressure for 60 min to induce retinal IR injury. Expression of macroglial and microglial cell markers (GFAP and Iba1), hypoxia inducing factor (HIF)-1α, and histone acetylation was analyzed after IR injury. To investigate the role of HDACs in the activation of glial cells, overexpression of HDAC1 and HDAC2 isoforms was performed. To determine the effect of HDAC inhibition on RGC survival, trichostatin-A (TSA, 2.5 mg/kg) was injected intraperitoneally. After IR injury, retinal GFAP, Iba1, and HIF-1α were upregulated. Conversely, retinal histone acetylation was downregulated. Notably, adenoviral-induced overexpression of HDAC2 enhanced glial activation following IR injury, whereas overexpression of HDAC1 did not significantly affect glial activation. TSA treatment significantly increased RGC survival after IR injury. Our results suggest that increased activity of HDAC2 is closely related to glial activation in a mouse model of retinal IR injury and inhibition of HDACs by TSA showed neuroprotective potential in retinas with IR injuries.
Neuroimmunology: What Role for Autoimmunity, Neuroinflammation, and Small Fiber Neuropathy in Fibromyalgia, Chronic Fatigue Syndrome, and Adverse Events after Human Papillomavirus Vaccination?
Authors: Ryabkova Varvara A. , Churilov Leonid P. , Shoenfeld Yehuda
Published: October 2019

Fibromyalgia is a disorder characterized by chronic widespread pain and non-pain symptoms, such as fatigue, dysautonomia, and cognitive and sleep disturbances. Its pathogenesis and treatment continue to be the subject of debate. We highlight the role of three mechanisms—autoimmunity, neuroinflammation, and small fiber neuropathy—in the pathogenesis of the disease. These mechanisms are shown to be closely interlinked (also on a molecular level), and the review considers the implementation of this relationship in the search for therapeutic options. We also pay attention to chronic fatigue syndrome, which overlaps with fibromyalgia, and propose a concept of “autoimmune hypothalamopathy” for its pathogenesis. Finally, we analyze the molecular mechanisms underlying the neuroinflammatory background in the development of adverse events following HPV vaccination and suggesting neuroinflammation, which could exacerbate the development of symptoms following HPV vaccination (though this is hotly debated), as a model for fibromyalgia pathogenesis.
Emerging Roles of DDB2 in Cancer
Authors: Gilson Pauline , Drouot Guillaume , Witz Andréa , Merlin Jean-Louis , Becuwe Philippe , Harlé Alexandre
Published: October 2019

Damage-specific DNA-binding protein 2 (DDB2) was originally identified as a DNA damage recognition factor that facilitates global genomic nucleotide excision repair (GG-NER) in human cells. DDB2 also contributes to other essential biological processes such as chromatin remodeling, gene transcription, cell cycle regulation, and protein decay. Recently, the potential of DDB2 in the development and progression of various cancers has been described. DDB2 activity occurs at several stages of carcinogenesis including cancer cell proliferation, survival, epithelial to mesenchymal transition, migration and invasion, angiogenesis, and cancer stem cell formation. In this review, we focus on the current state of scientific knowledge regarding DDB2 biological effects in tumor development and the underlying molecular mechanisms. We also provide insights into the clinical consequences of DDB2 activity in cancers.
Melatonin Deficiency Confers Tolerance to Multiple Abiotic Stresses in Rice via Decreased Brassinosteroid Levels
Authors: Hwang Ok Jin , Back Kyoungwhan
Published: October 2019

Melatonin has long been recognized as a positive signaling molecule and potent antioxidant in plants, which alleviates damage caused by adverse conditions such as salt, cold, and heat stress. In this study, we found a paradoxical role for melatonin in abiotic stress responses. Suppression of the serotonin N-acetyltransferase 2 (snat2) gene encoding the penultimate enzyme in melatonin biosynthesis led to simultaneous decreases in both melatonin and brassinosteroid (BR) levels, causing a semi-dwarf with erect leaf phenotype, typical of BR deficiency. Here, we further characterized snat2 rice in terms of grain morphology and abiotic stress tolerance, to determine whether snat2 rice exhibited characteristics similar to those of BR-deficient rice. As expected, the snat2 rice exhibited tolerance to multiple stress conditions including cadmium, salt, cold, and heat, as evidenced by decreased malondialdehyde (MDA) levels and increased chlorophyll levels, in contrast with SNAT2 overexpression lines, which were less tolerant to stress than wild type plants. In addition, the length and width of grain from snat2 plants were reduced relative to the wild type, which is reminiscent of BR deficiency in rice. Other melatonin-deficient mutant rice lines with suppressed BR synthesis (i.e., comt and t5h) also showed tolerance to salt and heat stress, whereas melatonin-deficient rice seedlings without decreased BR levels (i.e., tdc) failed to exhibit increased stress tolerance, suggesting that stress tolerance was increased not by melatonin deficiency alone, but by a melatonin deficiency-mediated decrease in BR.
Intracrine Endorphinergic Systems in Modulation of Myocardial Differentiation
Authors: Canaider Silvia , Facchin Federica , Tassinari Riccardo , Cavallini Claudia , Olivi Elena , Taglioli Valentina , Zannini Chiara , Bianconi Eva , Maioli Margherita , Ventura Carlo
Published: October 2019

A wide variety of peptides not only interact with the cell surface, but govern complex signaling from inside the cell. This has been referred to as an “intracrine” action, and the orchestrating molecules as “intracrines”. Here, we review the intracrine action of dynorphin B, a bioactive end-product of the prodynorphin gene, on nuclear opioid receptors and nuclear protein kinase C signaling to stimulate the transcription of a gene program of cardiogenesis. The ability of intracrine dynorphin B to prime the transcription of its own coding gene in isolated nuclei is discussed as a feed-forward loop of gene expression amplification and synchronization. We describe the role of hyaluronan mixed esters of butyric and retinoic acids as synthetic intracrines, controlling prodynorphin gene expression, cardiogenesis, and cardiac repair. We also discuss the increase in prodynorphin gene transcription and intracellular dynorphin B afforded by electromagnetic fields in stem cells, as a mechanism of cardiogenic signaling and enhancement in the yield of stem cell-derived cardiomyocytes. We underline the possibility of using the diffusive features of physical energies to modulate intracrinergic systems without the needs of viral vector-mediated gene transfer technologies, and prompt the exploration of this hypothesis in the near future.
The Analysis of Arterial Stiffness in Heart Failure Patients in Comparison with Healthy Subjects and Patients with Cardiovascular Risk Factors
Authors: Feola Mauro , Testa Marzia , Ferreri Cinzia , Rosso GianLuca , Rossi Arianna , Ruocco Gaetano
Published: October 2019

The arterial stiffness in the pathogenesis and clinical outcome in heart failure (HF) patients still needs to be clarified. An increased pulse wave velocity (PWV) in HF patients in comparison with healthy subjects and cardiovascular risk factors (CVRF) patients has been demonstrated. The aim of this study was to evaluate the arterial stiffness in HF patients in comparison to control populations. Methods: Consecutive patients admitted for decompensated heart failure underwent echocardiogram and evaluation of arterial stiffness by measuring the PWV and the augmentation index (AIx75). The arterial stiffness was also calculated in a control group formed by healthy volunteers and in CVRF subjects. Results: Fifty-nine HF patients (62% males; age 75 years) with mean left ventricular ejection fraction (LVEF) 38% and N-terminal pro B-type natriuretic peptide (NT-proBNP) (8111 pg/mL) entered the study. The HF population were compared with 22 healthy controls (age 58 years) and 20 CVRF patients (age 72 years). The analysis of PWV demonstrated a velocity of 10.6 m/s (9–12.1 m/s), 11.7 m/second (10.4–12.8 m/s), and 10.1 m/second (8.6–10.8m/s) in controls, CVRF, and HF patients (p = 0.01). AIx75 was seen to be higher in the CVRF group vs. HF patients (34% vs. 22%, p = 0.001). In HF patients PWV was inversely correlated with the glomerular filtration rate (r = –0.40; p = 0.002) and directly with central systolic pressure (SP) (r = 0.29; p = 0.02), brachial SP (r = 0.33; p = 0.01) as well as AIx75 correlated with GFR (r = −033; p = 0.01). Conclusion: PWV proved to be different in HF patients in comparison with CVRF/healthy population. The strongest correlation was revealed between the values of PWV/AIx75 and renal function.
Monitoring and Determining Mitochondrial Network Parameters in Live Lung Cancer Cells
Authors: Mirzapoiazova Tamara , Li Haiqing , Nathan Anusha , Srivstava Saumya , Nasser Mohd W. , Lennon Frances , Armstrong Brian , Mambetsariev Isa , Chu Peiguo G. , Achuthan Srisairam , Batra Surinder K. , Kulkarni Prakash , Salgia Ravi
Published: October 2019

Mitochondria are dynamic organelles that constantly fuse and divide, forming dynamic tubular networks. Abnormalities in mitochondrial dynamics and morphology are linked to diverse pathological states, including cancer. Thus, alterations in mitochondrial parameters could indicate early events of disease manifestation or progression. However, finding reliable and quantitative tools for monitoring mitochondria and determining the network parameters, particularly in live cells, has proven challenging. Here, we present a 2D confocal imaging-based approach that combines automatic mitochondrial morphology and dynamics analysis with fractal analysis in live small cell lung cancer (SCLC) cells. We chose SCLC cells as a test case since they typically have very little cytoplasm, but an abundance of smaller mitochondria compared to many of the commonly used cell types. The 2D confocal images provide a robust approach to quantitatively measure mitochondrial dynamics and morphology in live cells. Furthermore, we performed 3D reconstruction of electron microscopic images and show that the 3D reconstruction of the electron microscopic images complements this approach to yield better resolution. The data also suggest that the parameters of mitochondrial dynamics and fractal dimensions are sensitive indicators of cellular response to subtle perturbations, and hence, may serve as potential markers of drug response in lung cancer.
Long-Term Burden of Increased Body Mass Index from Childhood on Adult Dyslipidemia: The i3C Consortium Study
Authors: Yan Yinkun , Bazzano Lydia A. , Juonala Markus , Raitakari Olli T. , Viikari Jorma S. A. , Prineas Ronald , Dwyer Terence , Sinaiko Alan , Burns Trudy L. , Daniels Stephen R. , Woo Jessica G. , Khoury Philip R. , Urbina Elaine M. , Jacobs David R. , Hu Tian , Steinberger Julia , Venn Alison , Chen Wei
Published: October 2019

Background: Data are limited regarding the association of cumulative burden and trajectory of body mass index (BMI) from early life with adult lipid disorders. Methods: The study cohort consisted of 5195 adults who had BMI repeatedly measured 4 to 21 times from childhood and had blood lipid measurements of low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and triglycerides (TG) and information on lipid-lowering medications in the last adult survey. The area under the curve (AUC) was calculated as a measure of long-term burden (total AUC) and trends (incremental AUC) of BMI. Results: Participants with dyslipidemia, high LDL-C, low HDL-C and high TG had consistently and significantly higher BMI levels from childhood to adulthood compared to those with normal lipid levels. After adjusting for age, race, sex, and cohort, increased risk of adult dyslipidemia was significantly associated with higher values of childhood BMI, adulthood BMI, total AUC and incremental AUC, with odds ratio (95% confidence interval) = 1.22 (1.15–1.29), 1.85 (1.74–1.97), 1.61 (1.52–1.71), and 1.59 (1.50–1.69), respectively, and p < 0.001 for all. The association patterns were similar in most race–sex subgroups. Conclusions: Adults with dyslipidemia versus normal lipid levels have consistently higher levels and distinct life-course trajectories of BMI, suggesting that the impact of excessive body weight on dyslipidemia originates in early life.
Phenotypic Switching of Naïve T Cells to Immune-Suppressive Treg-Like Cells by Mutant KRAS
Authors: Kalvala Arjun , Wallet Pierre , Yang Lu , Wang Chongkai , Li Haiqing , Nam Arin , Nathan Anusha , Mambetsariev Isa , Poroyko Valeriy , Gao Hanlin , Chu Peiguo , Sattler Martin , Bild Andrea , Manuel Edwin R. , Lee Peter P. , Jolly Mohit Kumar , Kulkarni Prakash , Salgia Ravi
Published: October 2019

Oncogenic (mutant) Ras protein Kirsten rat sarcoma viral oncogene homolog (KRAS) promotes uncontrolled proliferation, altered metabolism, and loss of genome integrity in a cell-intrinsic manner. Here, we demonstrate that CD4+ T cells when incubated with tumor-derived exosomes from mutant (MT) KRAS non-small-cell lung cancer (NSCLC) cells, patient sera, or a mouse xenograft model, induce phenotypic conversion to FOXP3+ Treg-like cells that are immune-suppressive. Furthermore, transfecting T cells with MT KRAS cDNA alone induced phenotypic switching and mathematical modeling supported this conclusion. Single-cell sequencing identified the interferon pathway as the mechanism underlying the phenotypic switch. These observations highlight a novel cytokine-independent, cell-extrinsic role for KRAS in T cell phenotypic switching. Thus, targeting this new class of Tregs represents a unique therapeutic approach for NSCLC. Since KRAS is the most frequently mutated oncogene in a wide variety of cancers, the findings of this investigation are likely to be of broad interest and have a large scientific impact.
Radial Artery Access for Percutaneous Cardiovascular Interventions: Contemporary Insights and Novel Approaches
Authors: Scalise Renato Francesco Maria , Salito Armando Mariano , Polimeni Alberto , Garcia-Ruiz Victoria , Virga Vittorio , Frigione Pierpaolo , Andò Giuseppe , Tumscitz Carlo , Costa Francesco
Published: October 2019

Since its introduction, the transradial access for percutaneous cardiovascular procedures has been associated with several advantages as compared to transfemoral approach, and has become the default for coronary angiography and intervention. In the last 30 years, a robust amount of evidence on the transradial approach has been mounted, promoting its diffusion worldwide. This article provides a comprehensive review of radial artery access for percutaneous cardiovascular interventions, including the evidence from clinical trials of transradial vs. transfemoral approach, technical considerations, access-site complications and limitations, alternative forearm accesses (e.g., ulnar and distal radial artery), and ultimately the use of the radial approach for structural interventions.
Optically Transparent Metamaterial Absorber Using Inkjet Printing Technology
Authors: Jeong Heijun , Tentzeris Manos M. , Lim Sungjoon
Published: October 2019

An optically transparent metamaterial absorber that can be obtained using inkjet printing technology is proposed. In order to make the metamaterial absorber optically transparent, an inkjet printer was used to fabricate a thin conductive loop pattern. The loop pattern had a width of 0.2 mm and was located on the top surface of the metamaterial absorber, and polyethylene terephthalate films were used for fabricating the substrate. An optically transparent conductive indium tin oxide film was introduced in the bottom ground plane. Therefore, the proposed metamaterial absorber was optically transparent. The metamaterial absorber was demonstrated by performing a full-wave electromagnetic simulation and measured in free space. In the simulation, the 90% absorption bandwidth ranged from 26.6 to 28.8 GHz, while the measured 90% absorption bandwidth was 26.8–28.2 GHz. Therefore, it is successfully demonstrated by electromagnetic simulation and measurement results.
Theoretical Assessment of Thermodynamic Stability in Nanocrystalline Metallic Alloys
Authors: Locci Antonio Mario
Published: October 2019

Thermal stability in nanocrystalline alloys has been extensively explored while using both experimental and theoretical approaches. From the theoretical point of view, the vast majority of the models proposed in the literature have been implicitly limited to immiscible or dilute systems and thus lack the necessary generality to make predictions for different alloying interactions and in the case of intermetallic compounds formation. In this work, a general theoretical description for the case of binary W-based alloys is presented. It is shown that a critical value Ω ∗ of the interaction energy in the grain boundary Ω ( g b ) exists, such that the condition Ω ( g b ) < Ω ∗ can be regarded as a criterion for thermodynamic stability assessment. A procedure for calculating the value of Ω ∗ for each specific alloy is illustrated. A preliminary qualitative comparison between the model predictions and properly selected experimental findings taken from the literature and related to the W-Cr system is also provided.
Efficiency of Different Superplasticizers and Retarders on Properties of ‘One-Part’ Fly Ash-Slag Blended Geopolymers with Different Activators
Authors: Bong Shin Hau , Nematollahi Behzad , Nazari Ali , Xia Ming , Sanjayan Jay
Published: October 2019

Currently, there are a very limited number of studies on the effect of admixtures on properties of ‘one-part’ geopolymers. This paper reports the effects of different superplasticizers and retarders on fresh and hardened properties of one-part fly ash-slag blended geopolymers made by different solid activators. Two different grades of sodium silicate, namely anhydrous sodium metasilicate powder (nSiO2/nNa2O = 0.9) and GD Grade sodium silicate powder (nSiO2/nNa2O = 2.0) were used as the solid activators. Five different commercially available superplasticizers, including three modified polycarboxylate-based superplasticizers (denoted as PC1, PC2, and PC3) and two naphthalene-based superplasticizers (denoted as N1 and N2), as well as three different retarders, including sucrose, anhydrous borax and a commercially available retarder, were investigated. Workability, setting time and compressive strength of the mixtures without and with addition of each ‘individual’ admixture were measured. The results showed the effect of admixtures on the properties of the one-part geopolymers significantly depended on the type of solid activator and the type of admixture used. When GD Grade sodium silicate powder was used as the solid activator, all investigated admixtures not only had no positive effect on the workability and setting time, but also significantly reduced the compressive strength of the mixture. However, when anhydrous sodium metasilicate powder was used as the solid activator, the PC1 and sucrose were the best performing superplasticizer and retarder, respectively, causing no reduction in the compressive strength, but significant increase in the workability (up to + 72%) and setting time (up to + 111%), respectively as compared to the mixture with no admixture. In addition, the results also showed that addition of ‘combined’ admixtures (i.e., PC1 in the presence of sucrose) significantly increased the workability (up to + 39%) and setting time (up to + 141%), but slightly reduced the compressive strength (−16%) of the mixture activated by anhydrous sodium metasilicate powder, as compared to the mixture with no admixture.
Human Mesenchymal Stem Cell Response to Lactoferrin-based Composite Coatings
Authors: Icriverzi Madalina , Bonciu Anca , Rusen Laurentiu , Sima Livia Elena , Brajnicov Simona , Cimpean Anisoara , Evans Robert W. , Dinca Valentina , Roseanu Anca
Published: October 2019

The potential of mesenchymal stem cells (MSCs) for implantology and cell-based therapy represents one of the major ongoing research subjects within the last decades. In bone regeneration applications, the various environmental factors including bioactive compounds such as growth factors, chemicals and physical characteristics of biointerfaces are the key factors in controlling and regulating osteogenic differentiation from MSCs. In our study, we have investigated the influence of Lactoferrin (Lf) and Hydroxyapatite (HA) embedded within a biodegradable PEG-PCL copolymer on the osteogenic fate of MSCs, previous studies revealing an anti-inflammatory potential of the coating and osteogenic differentiation of murine pre-osteoblast cells. The copolymer matrix was obtained by the Matrix Assisted Pulsed Laser Evaporation technique (MAPLE) and the composite layers containing the bioactive compounds (Lf, HA, and Lf-HA) were characterised by Scanning Electron Microscopy and Atomic Force Microscopy. Energy-dispersive X-ray spectroscopy contact angle and surface energy of the analysed coatings were also measured. The characteristics of the composite surfaces were correlated with the viability, proliferation, and morphology of human MSCs (hMSCs) cultured on the developed coatings. All surfaces were found not to exhibit toxicity, as confirmed by the LIVE/DEAD assay. The Lf-HA composite exhibited an increase in osteogenic differentiation of hMSCs, results supported by alkaline phosphatase and mineralisation assays. This is the first report of the capacity of biodegradable composite layers containing Lf to induce osteogenic differentiation from hMSCs, a property revealing its potential for application in bone regeneration.
Vibration Viscosity Sensor for Engine Oil Monitoring Using Metal Matrix Piezoelectric Composite
Authors: Yanaseko Tetsuro , Sato Hiroshi , Kuboki Isao , Mossi Karla , Asanuma Hiroshi
Published: October 2019

Lubricants such as engine oil play an important role in preventing machine wear and damage. Monitoring the deterioration of lubricating oils is a significant technical issue in machine maintenance. In this study, a sensor for monitoring engine oil viscosity was developed using a metal-core piezoelectric fiber/aluminum composite. This composite is a piezoelectric ceramic that is reinforced by a metal matrix; it is expected to be utilized in harsh environments such as the inside of an engine. An active type measurement method was employed to monitor variations in the viscosity of glycerin solution as a model liquid. In this method, a self-generated vibration is correlated to the viscosity of a liquid by measuring the damped vibration amplitude and the variation in the resonance frequency. The results showed that the vibration had a high sensitivity to the liquid viscosity; further, it was observed that the shift in resonance frequency correlated to a wider range of measurable viscosity. Both measured parameters indicate that the metal-core piezoelectric fiber/aluminum composite is a viable sensor for engine oil monitoring.
Microstructure, Mechanical, and Corrosion Properties of Ni-Free Austenitic Stainless Steel Prepared by Mechanical Alloying and HIPping
Authors: Romanczuk Eliza , Perkowski Krzysztof , Oksiuta Zbigniew
Published: October 2019

An influence of the powder metallurgy route on the phase structure, mechanical properties, and corrosion resistance of Fe–18%Cr–12%Mn–N nickel-free austenitic stainless steel as a potential material for medical applications were studied. The powder was mechanically alloyed in a high purity nitrogen atmosphere for 90 h followed by Hot Isostatic Pressing at 1150 °C (1423 K) and heat treatment at 1175 °C (1423 K) for 1 h in a vacuum with furnace cooling and water quenching. More than 96% of theoretical density was obtained for the samples after Hot Isostatic Pressing that had a direct influence on the tensile strength of the tested samples (Ultimate Tensile Strength is 935 MPa) with the total elongation of 0.5%. Heat treatment did not affect the tensile strength of the tested material, however, an elongation was improved by up to 3.5%. Corrosion properties of the tested austenitic stainless steel in various stages of the manufacturing process were evaluated applying the anodic polarization measurements and compared with the austenitic 316LV stainless steel. In general, the heat treatment applied after Hot Isostatic Pressing improved the corrosion resistance. The Hot Isostatic Pressing sample shows dissolution, while heat treatment causes a passivity range, the noblest corrosion potential, and lower current density of this sample.
Inversely 3D-Printed β-TCP Scaffolds for Bone Replacement
Authors: Seidenstuecker Michael , Lange Svenja , Esslinger Steffen , Latorre Sergio H. , Krastev Rumen , Gadow Rainer , Mayr Hermann O. , Bernstein Anke
Published: October 2019

The aim of this study was to predefine the pore structure of β-tricalcium phosphate (β-TCP) scaffolds with different macro pore sizes (500, 750, and 1000 µm), to characterize β-TCP scaffolds, and to investigate the growth behavior of cells within these scaffolds. The lead structures for directional bone growth (sacrificial structures) were produced from polylactide (PLA) using the fused deposition modeling techniques. The molds were then filled with β-TCP slurry and sintered at 1250 °C, whereby the lead structures (voids) were burnt out. The scaffolds were mechanically characterized (native and after incubation in simulated body fluid (SBF) for 28 d). In addition, biocompatibility was investigated by live/dead, cell proliferation and lactate dehydrogenase assays. The scaffolds with a strand spacing of 500 µm showed the highest compressive strength, both untreated (3.4 ± 0.2 MPa) and treated with simulated body fluid (2.8 ± 0.2 MPa). The simulated body fluid reduced the stability of the samples to 82% (500), 62% (750) and 56% (1000). The strand spacing and the powder properties of the samples were decisive factors for stability. The fact that β-TCP is a biocompatible material is confirmed by the experiments. No lactate dehydrogenase activity of the cells was measured, which means that no cytotoxicity of the material could be detected. In addition, the proliferation rate of all three sizes increased steadily over the test days until saturation. The cells were largely adhered to or within the scaffolds and did not migrate through the scaffolds to the bottom of the cell culture plate. The cells showed increased growth, not only on the outer surface (e.g., 500: 36 ± 33 vital cells/mm² after three days, 180 ± 33 cells/mm² after seven days, and 308 ± 69 cells/mm² after 10 days), but also on the inner surface of the samples (e.g., 750: 49 ± 17 vital cells/mm² after three days, 200 ± 84 cells/mm² after seven days, and 218 ± 99 living cells/mm² after 10 days). This means that the inverse 3D printing method is very suitable for the presetting of the pore structure and for the ingrowth of the cells. The experiments on which this work is based have shown that the fused deposition modeling process with subsequent slip casting and sintering is well suited for the production of scaffolds for bone replacement.
Optimization of Power Consumption Associated with Surface Roughness in Ultrasonic Assisted Turning of Nimonic-90 Using Hybrid Particle Swarm-Simplex Method
Authors: Khanna Navneet , Airao Jay , Gupta Munish Kumar , Song Qinghua , Liu Zhanqiang , Mia Mozammel , Maruda Radoslaw , Krolczyk Grzegorz
Published: October 2019

These days, power consumption and energy related issues are very hot topics of research especially for machine tooling process industries because of the strict environmental regulations and policies. Hence, the present paper discusses the application of such an advanced machining process i.e., ultrasonic assisted turning (UAT) process with the collaboration of nature inspired algorithms to determine the ideal solution. The cutting speed, feed rate, depth of cut and frequency of cutting tool were considered as input variables and the machining performance of Nimonic-90 alloy in terms of surface roughness and power consumption has been investigated. Then, the experimentation was conducted as per the Taguchi L9 orthogonal array and the mono as well as bi-objective optimizations were performed with standard particle swarm and hybrid particle swarm with simplex methods (PSO-SM). Further, the statistical analysis was performed with well-known analysis of variance (ANOVA) test. After that, the regression equation along with selected boundary conditions was used for creation of fitness function in the subjected algorithms. The results showed that the UAT process was more preferable for the Nimconic-90 alloy as compared with conventional turning process. In addition, the hybrid PSO-SM gave the best results for obtaining the minimized values of selected responses.
A Series of Field-Induced Single-Ion Magnets Based on the Seven-Coordinate Co(II) Complexes with the Pentadentate (N3O2) H2dapsc Ligand
Authors: Kopotkov Vyacheslav A. , Korchagin Denis V. , Sasnovskaya Valentina D. , Gilmutdinov Ildar F. , Yagubskii Eduard B.
Published: October 2019

A series of five new mononuclear pentagonal bipyramidal Co(II) complexes with the equatorial 2,6-diacetylpyridine bis(semicarbazone) ligand (H2dapsc) and various axial pseudohalide ligands (SCN, SeCN, N(CN)2, C(CN)3, and N3) was prepared and structurally characterizated: [Co(H2dapsc)(SCN)2]∙0.5C2H5OH (1), [Co(H2dapsc)(SeCN)2]∙0.5C2H5OH (2), [Co(H2dapsc)(N(CN)2)2]∙2H2O (3), [Co(H2dapsc)(C(CN)3)(H2O)](NO3)∙1.16H2O (4), and {[Co(H2dapsc)(H2O)(N3)][Co(H2dapsc)(N3)2]}N3∙4H2O (5). The combined analyses of the experimental DC and AC magnetic data of the complexes (1–5) and two other earlier described those of this family [Co(H2dapsc)(H2O)2)](NO3)2∙2H2O (6) and [Co(H2dapsc)(Cl)(H2O)]Cl∙2H2O (7), their theoretical description and the ab initio CASSCF/NEVPT2 calculations reveal large easy-plane magnetic anisotropies for all complexes (D = + 35 − 40 cm−1). All complexes under consideration demonstrate slow magnetic relaxation with dominant Raman and direct spin–phonon processes at static magnetic field and so they belong to the class of field-induced single-ion magnets (SIMs).
Continuous Orbit Equivalence on Self-Similar Graph Actions
Authors: Yi Inhyeop
Published: October 2019

For self-similar graph actions, we show that isomorphic inverse semigroups associated to a self-similar graph action are a complete invariant for the continuous orbit equivalence of inverse semigroup actions on infinite path spaces.
Intraocular Pressure Measurements in Standing Position with a Rebound Tonometer
Authors: De Bernardo Maddalena , Borrelli Maria , Cembalo Giovanni , Rosa Nicola
Published: October 2019

Background and Objectives: It has been established that body position can play an important role in intraocular pressure (IOP) fluctuation. IOP has been previously shown to increase significantly when lying down, relative to sitting; this type of investigation has not been extensively reported for the standing (ST) position. Therefore, this study aims to look for eventual significant IOP changes while ST, sitting, and lying down. Materials and Methods: An Icare PRO was used to measure the IOP of 120 eyes of 60 healthy individuals, with age ranging from 21 to 55 years (mean 29.22 ± 9.12 years), in sitting, supine and ST positions; IOP was measured again, 5 min after standing (ST-5m). Results: Mean IOP difference between sitting and ST position was 0.39 ± 1.93 mmHg (95% CI: 0.04 to 0.74 mmHg) (p = 0.027); between sitting and ST-5m, it was −0.48 ± 1.79 mmHg (95% CI: −0.8 to −0.16 mmHg) (p = 0.004); between the sitting and supine position, it was −1.16±1.9 mmHg (95% CI: −1.5 to −0.82 mmHg) (p < 0.001); between the supine and ST position, it was 1.55 ± 2.04 mmHg (95% CI: 1.18 to 1.92 mmHg) (p < 0.001); between supine and ST-5m, it was 0.68 ± 1.87 mmHg (95% CI: 0.34 to 1.02 mmHg) (p < 0.001); and between ST-5m and ST, it was 0.94 ± 1.95 mmHg (95% CI: 0.58 to 1.29 mmHg) (p < 0.001). Mean axial eye length was 24.45 mm (95% CI: 24.22 to 24.69 mm), and mean central corneal thickness was 535.30 μm (95% CI: 529.44 to 541.19 μm). Conclusion: Increased IOP in the ST-5m position suggests that IOP measurements should be performed in this position too. The detection of higher IOP values in the ST-5m position than in the sitting one, may explain the presence of glaucoma damage or progression in apparently normal-tension or compensated patients.
Extraction of Mn from Black Copper Using Iron Oxides from Tailings and Fe2+ as Reducing Agents in Acid Medium
Authors: Pérez Kevin , Toro Norman , Campos Eduardo , González Javier , Jeldres Ricardo I. , Nazer Amin , Rodriguez Mario H.
Published: October 2019

Exotic type deposits include several species of minerals, such as atacamite, chrysocolla, copper pitch, and copper wad. Among these, copper pitch and copper wad have considerable concentrations of manganese. However, their non-crystalline and amorphous structure makes it challenging to recover the elements of interest (like Cu or Mn) by conventional hydrometallurgical methods. For this reason, black copper ores are generally not incorporated into the extraction circuits or left unprocessed, whether in stock, leach pads, or waste. Therefore, to dilute MnO2, the use of reducing agents is essential. In the present research, agitated leaching was performed to dissolve Mn of black copper in an acidic medium, comparing the use of ferrous ions and tailings as reducing agents. Two samples of black copper were studied, of high and low grade of Mn, respectively, the latter with a high content of clays. The effect on the reducing agent/black copper ratio and the concentration of sulfuric acid in the system were evaluated. Better results in removing Mn were achieved using the highest-grade black copper sample when working with ferrous ions at a ratio of Fe2+/black copper of 2/1 and 1 mol/L of sulfuric acid. Besides, the low-grade sample induced a significant consumption of H2SO4 due to the high presence of gangue and clays.
Varestraint Testing of Selective Laser Additive Manufactured Alloy 718—Influence of Grain Orientation
Authors: Raza Tahira , Andersson Joel , Svensson Lars-Erik
Published: October 2019

The effect of grain orientation on hot cracking susceptibility of selective laser additive manufactured Alloy 718 was investigated by Varestraint testing. Electron backscattered diffraction showed that cracks in heat affected zone (HAZ) of the welded samples occurred in high angle grain boundaries. The extent of HAZ cracking was smaller in samples tested parallel to the elongated grain orientation and larger in samples transverse to the elongated grain orientation. However, for solidification cracking in the weld metal, no significant difference with respect to grain orientation in the base metal was found.
Similarities in Metabolic Flexibility and Hunger Hormone Ghrelin Exist between FTO Gene Variants in Response to an Acute Dietary Challenge
Authors: Danaher Jessica , Stathis Christos G. , Cooke Matthew B.
Published: October 2019

The rs9939609 polymorphism of the fat mass and obesity-associated (FTO) gene has been associated with obesity, and studies have also shown that environmental/lifestyle interaction such as dietary intake might mediate this effect. The current study investigates the postprandial hormonal regulators of hunger and indirect markers of substrate utilisation and metabolic flexibility following a dietary challenge to determine if suppression of circulating ghrelin levels and/or reduced metabolic flexibility exist between FTO genotypes. One hundred and forty seven healthy, sedentary males and females (29.0 ± 0.7 yrs; 70.2 ± 1.1 kg; 169.1 ± 0.8 cm; 24.5 ± 0.3 kg/m2) complete a single experimental session. Anthropometric measures, circulating levels of active ghrelin, insulin and glucose, and substrate oxidation via indirect calorimetry, are measured pre-prandial and/or post-prandial. The FTO rs9939609 variant is genotyped using a real-time polymerase chain reaction. Metabolic flexibility (∆RER) is similar between FTO genotypes of the rs9939609 (T > A) polymorphism (p > 0.05). No differences in pre-prandial and/or postprandial substrate oxidation, plasma glucose, serum insulin or ghrelin are observed between genotypes (p > 0.05). These observations are independent of body mass index and gender. Altered postprandial responses in hunger hormones and metabolic flexibility may not be a mechanism by which FTO is associated with higher BMI and obesity in healthy, normal-weighted individuals.
Improving Cardiovascular Health through Nudging Healthier Food Choices: A Systematic Review
Authors: Tørris Christine , Mobekk Hilde
Published: October 2019

Obesity and metabolic syndrome are considered major public health problems, and their negative impact on cardiovascular disease (CVD) and diabetes mellitus type 2 (DM2) is profound. Targeting modifiable risk factors such as dietary habits is therefore of great importance. Many of today’s health challenges with overweight and obesity may have behavioral roots, and traditional methods such as regulations and campaigns are often insufficient to improve dietary choices. Nudging or choice architecture might be a viable tool to influence people’s everyday choices and behaviors to better outcomes. This paper reviews the current state of the rapidly expanding number of experimental field studies that investigate the effects/associations of nudging on healthy food choices. A systematic literature search was conducted in PubMed, where 142 citations were identified. Based on selection criteria, six randomized controlled trials and 15 non-randomized controlled trials were ultimately included. The results of this systematic review show that many of the studies included traffic-light labeling, which may be a promising strategy. The reviewed findings, however, also highlight the challenges that confront experimental studies examining the impact of nudging on diet.
Strange Stars in the Vector Interaction Enhanced Bag Model
Authors: Salinas Marc , Klähn Thomas , Jaikumar Prashanth
Published: October 2019

The vector interaction enhanced Bag model (vBag) for dense quark matter extends the commonly used thermodynamic Bag model (tdBag) by incorporating effects of dynamical chiral symmetry breaking (D χ SB) and vector repulsion. Motivated by the suggestion that the stability of strange matter is in tension with chiral symmetry breaking (D χ SB) we examine the parameter space for its stability in the vBag model in this work. Assuming the chiral transition occurs at sufficiently low density, we determine the stability region of strange matter as a function of the effective Bag constant and the vector coupling. As an astrophysical application, we construct contours of maximum mass M max and radius at maximum mass R max in this region of parameter space. We also study the stability of strange stars in the vBag model with maximum mass in the 2 M ⊙ range by computing the spectrum of radial oscillations, and comparing to results from the tdBag model, find some notable differences.
Formation and Maintenance of Tissue Resident Memory CD8+ T Cells after Viral Infection
Authors: Topham David J. , Reilly Emma C. , Emo Kris Lambert , Sportiello Mike
Published: October 2019

Tissue resident memory (TRM) CD8 T cells comprise a memory population that forms in peripheral, non-lymphoid tissues after an infection that does not recirculate into the bloodstream or other tissues. TRM cells often recognize conserved peptide epitopes shared among different strains of a pathogen and so offer a protective role upon secondary encounter with the same or related pathogens. Several recent studies have begun to shed light on the intrinsic and extrinsic factors regulating TRM. In addition, work is being done to understand how canonical “markers” of TRM actually affect the function of these cells. Many of these markers regulate the generation or persistence of these TRM cells, an important point of study due to the differences in persistence of TRM between tissues, which may impact future vaccine development to cater towards these important differences. In this review, we will discuss recent advances in TRM biology that may lead to strategies designed to promote this important protective immune subset.
Preparation and Characterization of Porous Ti/SnO2–Sb2O3/PbO2 Electrodes for the Removal of Chloride Ions in Water
Authors: Xu Kangdong , Peng Jianghua , Chen Pan , Gu Wankai , Luo Yunbai , Yu Ping
Published: October 2019

Porous Ti/SnO2–Sb2O3/PbO2 electrodes for electrocatalytic oxidation of chloride ions were studied by exploring the effects of different operating conditions, including pore size, initial concentration, current density, initial pH, electrode plate spacing, and the number of cycles. In addition, a physicochemical characterization and an electrochemical characterization of the porous Ti/SnO2–Sb2O3/PbO2 electrodes were performed. The results showed that Ti/SnO2–Sb2O3/PbO2 electrodes with 150 µm pore size had the best removal effect on chloride ions with removal ratios amounting up to 98.5% when the initial concentration was 10 g L−1, the current density 125 mA cm−2, the initial pH = 9, and the electrode plate spacing 0.5 cm. The results, moreover, showed that the oxygen evolution potential of 150 µm porous Ti/SnO2-Sb2O3/PbO2 electrodes was highest, which minimized side reactions involving oxygen formation and which increased the removal effect of chloride ions.
Absolute Stability Condition Derivation for Position Closed-Loop System in Hydraulic Automatic Gauge Control
Authors: Zhu Yong , Tang Shengnan , Wang Chuan , Jiang Wanlu , Zhao Jianhua , Li Guangpeng
Published: October 2019

In the metallurgical industry, hydraulic automatic gauge control (HAGC) is a core mechanism for thickness control of plates used in the rolling process. The stability of the HAGC system’s kernel position closed-loop is key to ensuring a process with high precision, speed and reliability. However, the closed-loop position control system is typically nonlinear, and its stability is affected by several factors, making it difficult to analyze instability in the system. This paper describes in detail the functioning of the position closed-loop system. A mathematical model of each component was established using theoretical analysis. An incremental transfer model of the position closed-loop system was also derived by studying the connections between each component. In addition, based on the derived information transfer relationship, a transfer block diagram of disturbance quantity of the system was established. Furthermore, the Popov frequency criterion method was introduced to ascertain its absolute stability. The results indicate that the absolute stability conditions of the position closed-loop system are derived in two situations: when spool displacement is positive or negative. This study lays a theoretical foundation for research on the instability mechanism of an HAGC system.