bluuebunny/crossref_metadata_2025 huggingface dataset:
Hospital Hygiene Paradox: MRSA and Enterobacteriaceae Colonization Among Cleaning Staff in a Tertiary Hospital in Saudi Arabia
Authors: Alqurashi Maher S. , Sawan Asma A. , Berekaa Mahmoud M. , Hunasemarada Basavaraja C. , Al Shubbar Mohammed D. , Al Qunais Abdulaziz A. , Huldar Ammar S. , Bojabara Loay M. , Alamro Saud A. , El-Badry Ayman A.
Published: February 2025

Background and Objectives: Despite extensive research on the sources and transmission pathways of Hospital-Acquired Infections (HAIs), the role of cleaning staff as potential vectors has been under-explored. This study addresses the gap by examining the cleaning staff’s role in nosocomial infection transmission, focusing on pathogenic bacteria and fungi colonization. Identifying potential pathogens harbored by cleaning staff that carry the risk of causing HAIs, especially methicillin-resistant Staphylococcus aureus (MRSA) and Enterobacteriaceae. Materials and Methods: This current cross-sectional study was conducted on 96 cleaning staff at King Fahad Hospital of the University and Family and Community Medicine-Centre, Eastern Province, Saudi Arabia. Sample collection targeted hands and nostrils using cotton swabs, followed by laboratory testing, including MALDI-TOF mass spectrometry for bacterial identification, and the cefoxitin disc diffusion test for the screening of MRSA. Results: In total, the occurrence of MRSA colonization was 9.4% while Enterobacteriaceae colonization was 15.6%. No significant correlation was found for MRSA among departments or between day and night shifts. Alternatively, individuals working in the ICU and the operating room showed higher chances of being colonized by Enterobacteriaceae, and a notable connection was identified between Enterobacteriaceae and day shifts. Ultimately, the frequency of handwashing reduced the chances of being colonized by MRSA and Enterobacteriaceae, underscoring the significance of good hygiene practices. Conclusions: The study highlights the potential role of cleaning staff in transmitting HAIs, indicating a need for further research and consideration of enhanced hygiene protocols in hospital settings.
Justice for Placebo: Placebo Effect in Clinical Trials and Everyday Practice
Authors: Knezevic Nebojsa Nick , Sic Aleksandar , Worobey Samantha , Knezevic Emilija
Published: February 2025

The placebo effect has been widely documented across various medical conditions, demonstrating its ability to influence both subjective and objective outcomes. Placebo responses can significantly improve symptoms in these different conditions, such as pain, Parkinson’s disease, depression, anxiety, and addiction. Psychological mechanisms, particularly the power of patient expectations, appear to play a central role, with neurobiological evidence supporting the activation of dopamine, endogenous opioids, and endocannabinoids in response to placebo interventions. Studies have demonstrated that placebo injections and more complex procedures, including sham surgeries, can produce therapeutic effects comparable to real treatments, particularly in pain management and neurological disorders. Moreover, placebo responses could be amplified when patients are aware of receiving treatment, as shown by research on open-label placebos and open versus hidden medical treatments. The effectiveness of 0.9% sodium chloride solution as a placebo in clinical trials is debated, with some studies indicating its potential to induce clinical improvements, though it may not be an ideal control in inflammatory pain conditions. Advances in neuroimaging have revealed that placebo treatments trigger tangible biological processes in the brain and body and are supported by psychological and physiological mechanisms that interact, suggesting real biological processes are involved in the observed effects. Overall, the growing understanding of placebo mechanisms suggests that incorporating placebo-based strategies, with patient awareness and appropriate ethical considerations, may offer significant potential for improving patient outcomes, particularly in chronic pain, mental health, and neurological conditions.
Investigation on the Mechanism of Hot Deformation Behavior of Aluminum Single Crystals Based on Grain Orientation
Authors: Pan Suping , Chen Baishan , Chen Yuqiang , Xu Jiabei , Lu Dingding , Liu Wenhui
Published: February 2025

Aluminum alloys are widely used in transportation industries due to their excellent specific strength, stiffness, and formability. Modifying the texture of aluminum alloys can further enhance their mechanical properties. This study explores the hot deformation behavior of high-purity aluminum single crystals (ASCs) with Brass and Goss orientations. We examine the influence of crystal orientation on deformation mechanisms and establish hot processing maps to identify optimal conditions for microstructural evolution. The results highlight the distinct behaviors of Goss- and Brass-oriented ASCs, with Goss exhibiting greater dynamic recrystallization potential and Brass showing higher strain rate sensitivity at elevated temperatures.
An In-Depth Review of Molecularly Imprinted Electrochemical Sensors as an Innovative Analytical Tool in Water Quality Monitoring: Architecture, Principles, Fabrication, and Applications
Authors: Mukendi Mbuyamba Divin , Salami Oluseyi Sikiru , Mketo Nomvano
Published: February 2025

Molecularly imprinted electrochemical sensors (MI-ECSs) are a significant advancement in analytical techniques, especially for water quality monitoring (WQM). These sensors utilize molecular imprinting to create polymer matrices that exhibit high specificity and affinity for target analytes. MI-ECSs integrate molecularly imprinted polymers (MIPs) with electrochemical transducers (ECTs), enabling the selective recognition and quantification of contaminants. Their design features template-shaped cavities in the polymer that mimic the functional groups, shapes, and sizes of target analytes, resulting in enhanced binding interactions and improved sensor performance in complex water environments. The fabrication of MI-ECSs involves selecting suitable monomeric units (monomers) and crosslinkers, using a target analyte as a template, polymerizing, and then removing the template to expose the imprinted sites. Advanced methodologies, such as electropolymerization and surface imprinting, are used to enhance their sensitivity and reproducibility. MI-ECSs offer considerable benefits, including high selectivity, low detection limits, rapid response times, and the potential for miniaturization and portability. They effectively assess and detect contaminants, like (toxic) heavy metals (HMs), pesticides, pharmaceuticals, and pathogens, in water systems. Their ability for real-time monitoring makes them essential for ensuring water safety and adhering to regulations. This paper reviews the architecture, principles, and fabrication processes of MI-ECSs as innovative strategies in WQM and their application in detecting emerging contaminants and toxicants (ECs and Ts) across various matrices. These ECs and Ts include organic, inorganic, and biological contaminants, which are mainly anthropogenic in origin and have the potential to pollute water systems. Regarding this, ongoing advancements in MI-ECS technology are expected to further enhance the analytical capabilities and performances of MI-ECSs to broaden their applications in real-time WQM and environmental monitoring.
Magnetic Excitation for Coupled Pendulum and Piezoelectric Wave Energy Harvester
Authors: Feng Wuwei , Luo Xiang , Yang Shujie , Zou Qingping
Published: February 2025

Wave energy is one of the most reliable and promising renewable energy sources that has attracted lots of attention, including piezoelectric wave energy harvesting devices. One of the challenges for piezoelectric wave power generation is the relatively low-frequency wave environments in the ocean. Magnetic excitations are one of the techniques used to overcome this issue. However, there is a lack of understanding of the mechanisms to maximize the electric power output of piezoelectric wave energy harvesters through magnetic excitations. In the present study, magnetic excitation experiments were conducted to investigate the power generation of a coupled spring pendulum piezoelectric energy harvester under various magnetic field conditions. Firstly, the mass of the load magnet that can induce the resonance phenomenon in piezoelectric elements was experimentally determined. Then, the power generation of piezoelectric elements was tested under different excitation magnetic spacings. Finally, the influence of different distribution patterns of excitation magnets on the performance of piezoelectric elements was tested. It was found that under the conditions of a load magnet mass of 2 g, excitation magnet spacing of 4 mm, and two excitation magnets stacked on the inner pendulum, optimum power generation of the piezoelectric wave harvester was achieved with a peak-to-peak output voltage of 39 V. The outcome of this study provides new insight for magnetic excitation devices for piezoelectric wave energy harvesting to increase the feasibility and efficiency of wave energy conversion to electrical energy.
Antimicrobial-Resistance and Virulence-Associated Genes of Pasteurella multocida and Mannheimia haemolytica Isolated from Polish Dairy Calves with Symptoms of Bovine Respiratory Disease
Authors: Lachowicz-Wolak Agnieszka , Chmielina Aleksandra , Przychodniak Iwona , Karwańska Magdalena , Siedlecka Magdalena , Klimowicz-Bodys Małgorzata , Dyba Kamil , Rypuła Krzysztof
Published: February 2025

Bovine respiratory disease causes significant economic losses in cattle farming due to mortality, treatment costs, and reduced productivity. It involves viral and bacterial infections, with Pasteurella multocida and Mannheimia haemolytica key bacterial pathogens. These bacteria contribute to severe pneumonia and are often found together. Poland has one of the highest levels of antimicrobial use in food-producing animals among European Union countries. A total of 70 bacterial strains were analyzed, 48 P. multocida and 22 M. haemolytica, collected from affected calves’ respiratory tracts. The bacterial species were confirmed molecularly using PCR, which was also employed to detect antimicrobial resistance and virulence-associated genes. Antimicrobial susceptibility was determined using the broth microdilution method. Antimicrobial resistance varied between the two bacterial species studied. The highest resistance in P. multocida was to chlortetracycline 79.2% (38/48) and oxytetracycline 81.3% (39/48), while M. haemolytica showed 63.6% (14/22) resistance to penicillin and tilmicosin. The highest susceptibility was found for fluoroquinolones: P. multocida demonstrated 91.7% (44/48) susceptibility to enrofloxacin and 87.5% (42/48) to danofloxacin, while 77.3% (17/22) of M. haemolytica were susceptible to both tested fluoroquinolones. The tetH and tetR genes were observed only in P. multocida, at frequencies of 20.8% (10/48) and 16.7% (8/48), respectively. Both species carried the mphE and msrE genes, though at lower frequencies. All M. haemolytica contained the lkt, gs60, and gcp genes. All P. multocida carried the sodA gene, while the hgbB and ompH genes were present in 37.5% (18/48) and 20.8% (10/48) of strains, respectively. The highest resistance was observed against the most commonly used antibiotics in the European Union, although the resistance differed between the studied bacterial species and each strain exhibited the presence of at least one virulence gene.
Metabolic Reprogramming in Response to Freund’s Adjuvants: Insights from Serum Metabolomics
Authors: Mone Kiruthiga , Garcia Eloy Jose Torres , Abdullatif Fatema , Rasquinha Mahima T. , Sur Meghna , Hanafy Mostafa , Zinniel Denise K. , Singh Shraddha , Thomas Raymond , Barletta Raul G. , Gebregiworgis Teklab , Reddy Jay
Published: February 2025

Freund’s adjuvants have been used in vaccine and autoimmune settings, and their effects can be overlapping or unique to each. While both incomplete Freund’s adjuvants (IFA) and complete Freund’s adjuvants (CFA) influence antibody and T cell responses, the robust T helper 1 cytokines induced by the mycobacterial components make CFA the powerful immunostimulating adjuvant. In these studies, the adjuvant effects are investigated in a select population of cells, and the changes, if any, with the metabolic alterations in the systemic compartment are unclear. We investigated whether the effects of IFA and CFA can be influenced by the metabolic shifts in mice immunized with saline, IFA, or CFA using Mycobacterium tuberculosis var. bovis Bacillus Calmette–Guérin (BCG) as a positive control. After seven days of immunization, we analyzed the serum metabolite profiles using liquid chromatography coupled with high-resolution mass spectrometry and multivariate statistical analysis to identify metabolic features between the groups. The data revealed that, in the scores space, the CFA and BCG groups were more closely aligned compared to the saline group, while the IFA group displayed an intermediate profile. Furthermore, comparisons between the CFA and BCG groups showed more significant perturbations in lipid and amino acid metabolism, particularly involving glycerophospholipids, cysteine, and aromatic amino acids. In contrast, comparisons between the BCG and IFA groups indicated a more pronounced disruption in central energy metabolism pathways, such as the citric acid cycle and pyruvate metabolism. Together, the data suggest that the serum metabolite profiles in response to IFA and CFA might play a role in modulating the immune responses.
Enhancing Bacillus thuringiensis Performance: Fertilizer-Driven Improvements in Biofilm Formation, UV Protection, and Pest Control Efficacy
Authors: Zhao Fan , Mao Yufei , Yang Jiahong , Yang Sheng , Guan Xiong , Wang Zixuan , Huang Tianpei
Published: February 2025

This study investigated the effects of fertilizers on the biofilm formation, ultraviolet (UV) resistance, and insecticidal activity of Bacillus thuringiensis (Bt). Bacillus thuringiensis, a widely used microbial pesticide, has a minimal environmental impact and is highly effective against specific pests but is susceptible to environmental factors in field applications. Bacterial biofilms provide protection for Bt, enhancing its survival and functionality in the environment. However, the mechanisms by which fertilizers regulate the characteristics of microbial pesticides and enhance biofilm formation are not well understood. This study evaluated the effects of six fertilizers on the bacterial biofilm formation, the UV resistance, and the insecticidal activities of Bt wettable powders. The results demonstrated that fertilizers significantly enhanced the performance of three Bt preparations (Lv’an, Kang’xin, and Lu’kang). A compound fertilizer with 8.346 g/L of KCl, 2.751 g/L of ZnSO4·7H2O, and 25.681 μL/mL of humic acid was identified by response surface optimization, achieving the maximum BBF formation with OD595 value of 2.738. Furthermore, KH2PO4, HA, and ZnSO4·7H2O notably improved the survivability of Bt preparations under prolonged UV exposure, with the compound fertilizer providing the greatest protection. What’s more, fertilizers reduced the LC50 values of all Bt preparations, with the compound fertilizer decreasing the LC50 of the Lv’an Bt wettable powder to 0.139 g/L, a 3.12-fold increase in efficacy. This study demonstrated that fertilizers significantly enhance the UV resistance and insecticidal activity of Bt wettable powders by promoting bacterial biofilm formation. Herein, this study provides new strategies and theoretical support for Bt applications in modern sustainable agriculture.
Mechanical, Seepage, and Energy Evolution Properties of Multi-Shaped Fractured Sandstone Under Hydro-Mechanical Coupling: An Experimental Study
Authors: Zhang Ying , He Kai , Yang Jianming , Pan Jiliang , Xi Xun , Feng Xianhui , Zhang Leiming
Published: February 2025

Rocks with multi-shaped fractures in engineering activities like mining, underground energy storage, and hydropower construction are often exposed to environments where stress and seepage fields interact, which heightens the uncertainty of instability and failure mechanisms. This has long been a long-standing challenge in the field of rock mechanics. Current research mainly focuses on the mechanical behavior, seepage, and energy evolution characteristics of single-fractured rocks under hydro-mechanical coupling. However, studies on the effects of multi-shaped fractures (such as T-shaped fractures, Y-shaped fractures, etc.) on these characteristics under hydro-mechanical coupling are relatively scarce. This study aims to provide new insights into this field by conducting hydro-mechanical coupling tests on multi-shaped fractured sandstones (single fractures, T-shaped fractures, Y-shaped fractures) with different inclination angles. The results show that hydro-mechanical coupling significantly reduces the peak strength, damage stress, crack initiation stress, and closure stress of fractured sandstone. The permeability jump factor (ξ) demonstrates the permeability enhancement effects of different fracture shapes. The ξ values for single fractures, T-shaped fractures, and Y-shaped fractures are all less than 2, indicating that fracture shape has a relatively minor impact on permeability enhancement. Fracture inclination and shape significantly affect the energy storage capacity of the rock mass, and the release of energy exhibits a nonlinear relationship with fracture propagation. An in-depth analysis of energy evolution characteristics under the influence of fracture shape and inclination reveals the transition pattern of the dominant role of energy competition in the progressive failure process. Microstructural analysis of fractured sandstone shows that elastic energy primarily drives fracture propagation and the elastic deformation of grains, while dissipative energy promotes particle fragmentation, grain boundary sliding, and plastic deformation, leading to severe grain breakage. The study provides important theoretical support for understanding the failure mechanisms of multi-shaped fractured sandstone under hydro-mechanical coupling.
The Role of Ferulic Acid in Selected Malignant Neoplasms
Authors: Markowska Anna , Markowska Janina , Stanisławiak-Rudowicz Joanna , Kozak Katarzyna , Roubinek Otton Krzysztof , Jasińska Magdalena
Published: February 2025

Ferulic acid (FA) is a polyphenol that is found in plants and fruits. It has a wide range of anticancer properties, including participating in cell apoptosis, inhibiting invasion and angiogenesis, and acting synergistically with standard cytostatic agents in malignant tumors. A range of molecular mechanisms are involved in anticancer activity and include the following ones: activation of cell-cycle-related proteins and enzymes such as p53, p21, Bax, and pro-caspases 3 and 9, reduction of cyclin D1 and E, proapoptotic Bcl-2, MMP-9, and NF-kV, which decrease VEGF, leading to cell cycle arrest at G0/G1 phase and death of cancer cells. Other mechanisms inhibit several pathways: PI3K/AKT/mTOR, Notch, and Wnt, which are associated with downregulation of proliferation, invasion, metastasis, and angiogenesis. FA can induce activation of ROS, leading to DNA damage in cancer cells. In vitro and in vivo studies have demonstrated the significant antitumor activity of FA in breast cancer, particularly when used in combination with cytostatic agents. In vitro studies on cervical cancer cell lines have reported similar anticancer activity of FA. This includes inhibition of cell proliferation and induction of apoptosis by downregulating antiapoptotic proteins. A case-control study conducted in Italy found that men with histologically confirmed prostate cancer had notably lower levels of FA compared to controls. Molecular in vitro studies have suggested that FA may have various effects on the signaling pathways linked to a reduction in the risk of prostate cancer, and it may act in synergy with δ-tocotrienol, which is a derivative of vitamin E. In vivo and in vitro studies on colorectal cancer have demonstrated the effects of FA on the early development of this cancer—inhibition of abnormal crypt foci (ACF-aberrant crypt foci), as well as the reduction in cancer cell viability and apoptosis through molecular changes, mainly a decrease in EGFR expression. The poor water solubility of FA makes it an attractive candidate for use as nanoparticles.
Small Phenolic Metabolites at the Nexus of Nutrient Transport and Energy Metabolism
Authors: Mhawish Reham , Komarnytsky Slavko
Published: February 2025

Over time, human metabolism evolved to accommodate the challenges and benefits of plant foods that contain high amounts of carbohydrates and polyphenols. The latter are typically metabolized into small phenolic metabolites, including phenolic acids and their endogenous and microbial derivatives, that influence interconnected metabolic pathways involved in nutrient transport, energy metabolism, and neurotransmitter balance. Unlike other natural products, their biological effects arise from weak interactions with multiple molecular pathways rather than a single high-affinity receptor, making them versatile regulators of metabolic health. These compounds also modulate glucose transporters and carbohydrate metabolism, playing a crucial role in postprandial glucose and insulin responses. This review addresses the critical role of phenolic metabolites in metabolic health, with a focus on glucose homeostasis, insulin sensitivity, and carbohydrate metabolism. Incorporating polyphenols and phenolic acids into dietary strategies offers significant potential for improving insulin sensitivity, reducing metabolic disorder risks, and promoting whole-body glucose homeostasis. Furthermore, understanding how phenolic metabolites interact with metabolic pathways is essential for developing future effective nutritional strategies to support metabolic health.
One-Step Solvothermal Synthesis of Fe3O4 Acicular Aggregates Induced by Reaction Medium and Urea for Photocatalytic Degradation of Azo Dyes
Authors: Xu Yaohui , Li Yuting , Hou Quanhui , Gao Liangjuan , Ding Zhao
Published: February 2025

Based on the magnetic sensitivity of Fe3O4 in various fields, we aimed to propose a one-step solvothermal process for the synthesis of single-phase Fe3O4 induced by the reaction medium and urea, avoiding high-temperature reduction in H2 or N2 atmospheres. Feasibility was tested with purified water (H2O), methyl alcohol (MA), ethyl alcohol (EA), and ethylene glycol (EG) as reaction media. The findings indicated that the solvothermal reaction system utilizing EA was more effective for the synthesis of cubic magnetic Fe3O4. Optimal conditions for synthesizing pure Fe3O4 were obtained by optimizing the urea amount and solvothermal reaction parameters. The optimal formulation consisted of 10 mmol of FeCl3, 80 mmol of urea, and 60 mL of EA subjected to a solvothermal process at 200 °C for 12 h. The resulting Fe3O4 (magnetite, cubic) exhibited commendable crystallization with a morphology of acicular aggregates and displayed excellent magnetic sensitivity properties with a magnetization of 92.2 emu/g at 15,000 Oe. The photocatalytic degradation behaviors of the resulting Fe3O4 to Methyl Orange, Orange G, and Acid Red 37 azo dyes and the repeated degradation performance of Methyl Orange dye were investigated. Nearly complete degradation of Methyl Orange dye occurred after 2.0 h of photocatalytic reaction, while Orange G and Acid Red 37 dyes achieved similar results after 3.5 and 4.5 h, respectively. The exploration strategy in this work for synthesizing magnetic Fe3O4 can be applied to design and fabricate other metal oxides or composites, potentially resulting in novel discoveries in morphology or performance.
Electrolyte Gated Transistors for Brain Inspired Neuromorphic Computing and Perception Applications: A Review
Authors: Wang Weisheng , Zhu Liqiang
Published: February 2025

Emerging neuromorphic computing offers a promising and energy-efficient approach to developing advanced intelligent systems by mimicking the information processing modes of the human brain. Moreover, inspired by the high parallelism, fault tolerance, adaptability, and low power consumption of brain perceptual systems, replicating these efficient and intelligent systems at a hardware level will endow artificial intelligence (AI) and neuromorphic engineering with unparalleled appeal. Therefore, construction of neuromorphic devices that can simulate neural and synaptic behaviors are crucial for achieving intelligent perception and neuromorphic computing. As novel memristive devices, electrolyte-gated transistors (EGTs) stand out among numerous neuromorphic devices due to their unique interfacial ion coupling effects. Thus, the present review discusses the applications of the EGTs in neuromorphic electronics. First, operational modes of EGTs are discussed briefly. Second, the advancements of EGTs in mimicking biological synapses/neurons and neuromorphic computing functions are introduced. Next, applications of artificial perceptual systems utilizing EGTs are discussed. Finally, a brief outlook on future developments and challenges is presented.
Comparative In Vitro Drug Susceptibility Study of Five Oxazolidinones Against Mycobacterium tuberculosis in Hainan, China
Authors: Dong Jinhui , Cheng Qian , Tang Chuanning , Zhong Yeteng , Wang Jieying , Lv Meiping , Chen Zhuolin , Li Peibo , Luo Ming , Pei Hua
Published: February 2025

Oxazolidinones, novel synthetic antibacterials, inhibit protein biosynthesis and show potent activity against Gram-positive bacteria, including Mycobacterium tuberculosis (MTB). In this study, we aimed to compare the in vitro activity of linezolid (LZD) and four oxazolidinones, including tedizolid (TZD), contezolid (CZD), sutezolid (SZD), and delpazolid (DZD), against multidrug-resistant tuberculosis (MDR-TB) and pre-extensively drug-resistant tuberculosis (pre-XDR-TB) isolates from Hainan. We established their epidemiological cut-off values (ECOFFs) using ECOFFinder software and analyzed mutations in rrl (23S rRNA), rplC, rplD, mce3R, tsnR, Rv0545c, Rv0930, Rv3331, and Rv0890c genes to uncover potential mechanisms of oxazolidinone resistance. This study included 177 MTB isolates, comprising 67 MDR and 110 pre-XDR-TB isolates. Overall, SZD exhibited the strongest antibacterial activity against clinical MTB isolates, followed by TZD and LZD, with CZD and DZD showing equivalent but weaker activity (SZDMIC50 = TZDMIC50 < LZDMIC50 < CZDMIC50 = DZDMIC50; SZDMIC90 < TZDMIC90 = LZDMIC90 < CZDMIC90 = DZDMIC90). Significant differences in MIC distribution were observed for TZD (p < 0.0001), CZD (p < 0.01), SZD (p < 0.0001), and DZD (p < 0.0001) compared to LZD but not between MDR-TB and pre-XDR-TB isolates. We propose the following ECOFFs: SZD, 0.5 µg/mL; LZD, TZD, and CZD, 1.0 µg/mL; DZD, 2.0 µg/mL. No statistically significant differences in resistance rates were observed among these five drugs (p > 0.05). We found that eight MTB isolates (4.52% [8/177]) resisted these five oxazolidinones. Among these, only one isolate, M26, showed an amino acid substitution (Arg79His) in the protein encoded by the rplD gene, which conferred cross-resistance to TZD and CZD. Three distinct mutations were identified in the mce3R gene; notably, isolate P604 displayed two insertions that contributed to resistance against all five oxazolidinones. However, no significant correlation was observed between mutations in the rrl, rplC, rplD, mce3R, tsnR, Rv0545c, Rv0930, Rv3331, and Rv0890c genes with oxazolidinone resistance in the clinical MTB isolates tested. In summary, this study provides the first report on the resistance of MTB in Hainan to the five oxazolidinones (LZD, TZD, CZD, SZD, and DZD). In vitro susceptibility testing indicated that SZD exhibited the strongest antibacterial activity, followed by TZD and LZD, while CZD and DZD demonstrated comparable but weaker effectiveness. Mutations in rplD and mce3R were discovered, but further research is needed to clarify their role in conferring oxazolidinone resistance in MTB.
A Combination of Flavonoids Suppresses Cell Proliferation and the E6 Oncogenic Pathway in Human Papillomavirus-Transformed Cells
Authors: De Marco Federico , Altieri Fabio , Giuliani Stefano , Falcone Italia , Falcucci Susanna , Tedesco Mariassunta , Becelli Roberto
Published: February 2025

Despite the availability of excellent HPV-specific vaccines, HPV-related conditions and, notably, their related neoplastic diseases are expected to impact human health for many years to come. Polyphenols and flavonoids are a large class of natural products, credited with a wide range of pharmacological properties including antineoplastic activity. However, the currently available data depict a rather heterogeneous and sometimes contradictory landscape, and no univocal conclusions can be drawn. To shed light on such a controversial issue, a restricted list of promising polyphenols were evaluated for their antineoplastic activity on HPV-transformed cells. Among them, Kaempferol, Galangin, and Luteolin proved to have distinct anti-clonal activity with ID50 values, respectively, of 1.25, 6.25, and 3.0 microMolar, and three other compounds, namely, Chrysin, Quercetin, and Apigenin, showed fair although less intense activity with ID values, respectively, of 25.0, 40, and 25 microMolar. Interestingly, a distinct anti-proliferative effect could also be suggested for Kaempferol, Luteolin, and Apigenine. Cooperative anti-clonal effects could be suggested for binary and ternary compositions made of Kaepferol, Galangin, and Luteolin once combined at concentrations ranging from 2 to 8 microMolar. At these concentrations, the single components and the triple combination induced distinct cell cycle modulation associated with marked restoration of the p53 and p21Cip1/Waf1 levels, consistent with the disruption of a fundamental oncogenic pathway, the continuous activity of which is necessary for both the induction and maintenance of the viral-induced neoplastic phenotype.
Efficacy and Safety of Intra-Articular Therapy with Cross-Linked Hyaluronic Acid in Patients with Knee Osteoarthritis
Authors: Rania Vincenzo , Marcianò Gianmarco , Vocca Cristina , Palleria Caterina , Bianco Luigi , Caroleo Maria Cristina , Gallelli Luca
Published: February 2025

Background/Objectives: Knee osteoarthritis (OA) is a degenerative chronic disease characterized by a reduction in articular cartilage, as well as pain and functional limitations. We evaluated both the efficacy and safety of cross-linked high-molecular-weight hyaluronic acid in patients with knee OA. Methods: In this observational prospective single-arm study, a cross-linked high-molecular-weight hyaluronic acid (DIART ONE 90 mg in 3 mL) was administered in single injections to 50 patients aged 18–65 years, with a follow-up at 3, 6, and 12 months. Several scores were evaluated, including the Knee Injury and Osteoarthritis Outcome Score as the primary outcome measure and the Visual Analogue Scale, Timed Up and Go Test, Six-Minute Walking Test, General Health Assessment with 36-Item Short Form Health Survey, Zung’s Self-Rating Anxiety Scale, and Zung’s Self-Rating Depression Scale as secondary outcome measures. Both physicians and patients knew the kind of treatment they received. Results: During the follow-ups, we observed a statistically significant improvement in clinical scores at 3 and 6 months, with a decrease in clinical benefit at 12 months. Functional and psychological benefits were significant at 3, 6, and 12 months. No side effects were described except pain associated with the site of injection. Conclusions: In conclusion, we documented that cross-linked high-molecular-weight hyaluronic acid (DIART ONE 90 mg in 3 mL) represents an effective option in the management of mild–moderate osteoarthritis.
The Regulatory Role of CTCF in IL6 Gene Transcription Assessed in Breast Cancer Cell Lines
Authors: Pacheco-Hernandez Angel Francisco , Rodriguez-Ramos Itayesitl , Vazquez-Santillan Karla , Valle-Rios Ricardo , Velasco-Velázquez Marco , Aquino-Jarquin Guillermo , Martínez-Ruiz Gustavo Ulises
Published: February 2025

Background: Breast cancer (BrCa) patients with tumors expressing high interleukin-6 (IL6) levels have poor clinical outcomes. In BrCa, altered occupancy of CCCTC-binding factor (CTCF) within its DNA binding sites deregulates the expression of its targeted genes. In this study, we investigated whether CTCF contributes to the altered IL6 expression in BrCa. Methods/Results: We performed CTCF gain- and loss-of-function assays in BrCa cell lines and observed an inverse correlation between CTCF and IL6 expression levels. To understand how CTCF negatively regulates IL6 gene expression, we performed luciferase gene reporter assays, site-directed mutagenesis assays, and chromatin immunoprecipitation assays. Our findings revealed that CTCF interacted with the IL6 promoter, a form of regulation disrupted in a CpG methylation-independent fashion in MDA-MB-231 and Tamoxifen-resistant MCF7 cells. Data from TCGA and GEO databases allowed us to explore the clinical implications of our results. An inverse correlation between CTCF and IL6 expression levels was seen in disease-free survival BrCa patients but not in patients who experienced cancer recurrence. Conclusions: Our findings provide evidence that the CTCF-mediated negative regulation of the IL6 gene is lost in highly tumorigenic BrCa cells.
Hot Melt Extrusion as Continuous Manufacturing Technique to Produce Bilayer Films Loaded with Paracetamol or Lactase
Authors: Brokmann Friederike , Luthe Katharina , Hartmann Jonas , Müller Laura , Klammt Friederike , Hoffmann Carla , Weitschies Werner , Rosenbaum Christoph
Published: February 2025

Background/Objectives: The oral delivery of large-molecule drugs remains challenging due to poor solubility, perdemeability, and stability in the gastrointestinal tract, resulting in low bioavailability. In this study, hot melt extrusion (HME) was investigated as a solvent-free manufacturing technique for mucoadhesive bilayer films to improve drug absorption. Methods: Polyvinyl alcohol (PVA) and polyethylene oxide (PEO) were evaluated as mucoadhesive film-forming polymers, in conjunction with Eudragit® RS as a water-insoluble backing layer. Paracetamol and lactase were utilized as small and large molecule APIs, respectively. The resulting films were assembled into bilayer film samples and examined for mechanical properties, mucoadhesion, and dissolution behavior. A novel dissolution model was developed to evaluate unidirectional drug transport. Results: The results showed that bilayer films could be successfully fabricated using HME, with different mechanical properties depending on the polymer and drug content. Tests with the newly developed dissolution model showed a unidirectional drug release. The model also confirmed the need for biorelevant dissolution test systems because of a better differentiation between polymers compared to standard test methods such as the paddle-over-disk method. Furthermore, the investigation revealed that the activity of enzymes was retained after extrusion, thus indicating the feasibility of processing biologics. Conclusions: This study highlights the potential of HME to produce bilayer films as an innovative drug delivery platform offering improved bioavailability for both small and large molecules.
Treatment Strategies for Chronic Pancreatitis (CP)
Authors: Tłustochowicz Katarzyna , Krajewska Agnieszka , Kowalik Adrianna , Małecka-Wojciesko Ewa
Published: February 2025

Chronic pancreatitis (CP) and autoimmune pancreatitis (AIP) are diseases with overlapping features, both requiring complex management strategies. CP is characterized by pancreatic exocrine insufficiency (PEI) and pain, with treatment focused on symptom relief through pancreatic enzyme replacement therapy (PERT), pain control, and lifestyle and nutritional changes. However, the standard therapy does not address the underlying inflammation or fibrosis, which drives disease progression. AIP, on the other hand, presents with obstructive jaundice and fibrosis and is classified into two subtypes: Type 1 (AIP-1), linked to IgG4-related disease, and Type 2 (AIP-2), associated with inflammatory bowel disease. Treatment for AIP typically involves oral steroids. Immunomodulators and rituximab are used for recurrent or refractory cases. Novel therapies targeting the inflammation and fibrotic components of CP are being explored. A multidisciplinary approach is essential to optimize care and improve patients’ outcomes.
A Framework for Iterative Phase Retrieval Technique Integration into Atmospheric Adaptive Optics—Part II: High Resolution Wavefront Control in Strong Scintillations
Authors: Vorontsov Mikhail A. , Polnau Ernst
Published: February 2025

In this paper, we introduce atmospheric adaptive optics (AO) system architectures that utilize scintillation-resistant wavefront sensors based on iterative phase retrieval (IPR) techniques (described in detail in Part I) for closed-loop mitigation of atmospheric turbulence-induced wavefront aberrations in strong intensity scintillation conditions. The objective is to provide a framework (mathematical and numerical models, performance metrics, control algorithms, and wave-optics modeling and simulation results) for the potential integration of IPR-based wavefront sensing techniques into the following major atmospheric optics system types: directed energy laser beam projection, remote laser power delivery (remote power beaming), and free-space optical communications. Theoretical analysis and numerical simulation results demonstrate that the proposed closed-loop AO system architectures and control algorithms can be uniquely applicable for addressing one of the most challenging AO problems of turbulence effects mitigation in the presence of strong-intensity scintillations.
Silicon Photonics Devices and Integrated Circuits
Authors: Li Wei , Huang Duan
Published: February 2025

The rapid evolution of integrated photonics has ushered in a transformative era for optical communication and information processing systems, with silicon-based optical chips emerging as a cornerstone technology [...]
Experimental Study of an Efficient, High Power and Tunable Continuous-Wave CO2 Laser
Authors: Geng Lijie , Song Shuaifei , Yang Kun , Yan Pengji , Fu Zhenxiang , Qu Yanchen , Zhang Ruiliang , Zhang Zhifeng
Published: February 2025

The tunable CO2 laser is a common pump source for optically pumped terahertz lasers. In this paper, based on a sealed-off CO2 laser tube and a grating-tuned cavity, we reported an efficient, high-power, low-cost, simple structure and tunable continuous-wave CO2 laser. A sealed-off CO2 laser tube was designed and customized, and its plasma discharge characteristics were experimentally analyzed. The influence of output coupler transmittance and discharge current on the laser’s tunability and output power was systematically studied using grating tuning. A total of 78 spectral lines were achieved within the wavelength range of 9.17–10.86 μm. The maximum output power of 55.5 W was recorded on the 10P20 line when the output coupler transmittance was approximately 22%. The laser exhibited a beam spot radius of about 3.8 mm and a beam quality factor (M2) of 1.74.
Transcriptome Profiling Reveals the Effects of Rootstocks on Scion Architecture in Malus domestica Borkh Var. ‘Harlikar’
Authors: Xie Bin , Li Junhao , Zhou Jiangtao , Kang Guodong , Tang Zhongwen , Ma Xiaojian , Li Xin , Wang Jing , Zhang Yanzhen , Chen Yanhui , Yang Sumiao , Cheng Cungang
Published: February 2025

Rootstocks largely determine the tree architecture of the grafted scions, significantly affects yield, suitability for mechanical harvesting, and planting pattern of apple orchards. It is thus important to reveal the mechanisms behind the rootstocks influence on the tree architecture of scions in apple trees. This study analyzed the grafting survival rate, the physiological parameters including plant growth, photosynthesis and nutrient accumulation in the apple variety ‘Harlikar’ with eight apple rootstocks. We also explored the mechanism of scion architecture formation using transcriptomics based on different scion/rootstock combinations. The results indicated that ‘Harlikar’ had the lowest grafting survival rate with rootstock ‘M26’, with less callus formed at the graft interface, foliage etiolation, and weak photosynthetic capacity. While ‘Harlikar’ had better affinities with ‘M9-T337’, ‘M9-Nic29’, ‘M9-Pajam2’, ‘B9’, ‘71-3-150’, ‘Qingzhen 2’, and ‘Malus baccata’. Among these, the highest plant height and the highest number of lateral branches were observed in ‘Harlikar’ with rootstock ‘Qingzhen 2’, they were 1.12-times and 2.0-times higher than ‘Harlikar’ with vigorous rootstock ‘M. baccata’, respectively. The highest accumulations of total nitrogen, total phosphorus, and total potassium in scions were observed in ‘Harlikar’/‘Qingzhen 2’, they were 2.22-times, 2.10-times, and 11.80-times higher than that in ‘Harlikar’/‘M. baccata’. The lowest plant height was observed in ‘Harlikar’/‘71-3-150’, only 50.47% of ‘Harlikar’/‘Qingzhen 2’ and 56.51% of ‘Harlikar’/‘M. baccata’, and the lowest internode length was observed in ‘Harlikar’/‘M9-Nic29’, only 60.76% of ‘Harlikar’/‘Qingzhen 2’ and 79.11% of ‘Harlikar’/‘M. baccata’. The transcriptome, weighted gene co-expression network and KEGG enrichment analyses revealed that, compared to ‘Harlikar’/‘M. baccata’, most differentially expressed genes screened from ‘Harlikar’/‘Qingzhen 2’, ‘Harlikar’/‘71-3-150’, and ‘Harlikar’/‘M9-Nic29’ were enriched in hormone signal transduction pathways. Specifically, auxin-repressed protein gene ARP, cytokinin synthesis related genes CKXs and CYP92A6, and brassinosteroid synthesis related gene CYP87A3 were involved in the dwarfing of ‘Harlikar’/‘71-3-150’ and ‘Harlikar’/‘M9-Nic29’. Cytokinin synthesis related gene ARR-A and abscisic acid-responsive element binding factor gene ABF were the key to increased branching in ‘Harlikar’/‘Qingzhen 2’. In addition, acid phosphatase genes ACPs, and serine/threonine-protein kinase genes PBLs were involved in the vegetative growth of scions in ‘Harlikar’/‘Qingzhen 2’ by affecting the absorption and utilization of nutrients. These results provide theoretical guidance for cultivating high-quality ‘Harlikar’ apple trees and elucidate the molecular mechanisms regulating plant height and lateral branch formation in apple.
Effect of Ultra-High Temperature Degradation on the Physical Properties and Chemical Structure of an AMPS-Based Copolymer Oil-Well Cement Additive PADIM in Aqueous Solution
Authors: Yu Yongjin , Zhang Hang , Xu Pu , Zhang Xinyang , Wang Haige , Hu Miaomiao , Guo Jintang
Published: February 2025

2-acrylamido-2-methylpropane sulfonic acid (AMPS) based copolymer fluid loss agent is a kind of widely utilized additive in oil-well cement. However, when applied in ultra-high temperature (UHT) formation environment, its fluid loss control efficiency is significantly declined due to the thermal degradation behavior, and corresponding mechanism study is still lacking. Regarding the above issue, this work synthesized one representative copolymer fluid loss agent PADIM and investigated its thermal degradation mechanism in UHT aqueous environment, which was polymerized by AMPS, N, N-dimethylacrylamide (DMAA), itaconic acid (IA) and methacryloxyethyltrimethyl ammonium chloride (MTC). The aim of this paper was to provide a theoretical guidance for the futural structural design of the fluid loss agents for oil well cement slurry at UHTs. The copolymer solution was subjected to isothermal aging at 180–240 °C for 1.5 h or 6.0 h (to simulate short-period and long-period aging, respectively), and the aged products were further analyzed. It was found that the thermal decomposition onset temperature of the copolymer solid was 294.6 °C. However, its thermal stability in aqueous solution was significantly lower, with substantial main chain breakage and functional group transformations occurring below 240 °C. As a result, the apparent viscosity and average molecular weight were significantly reduced from 4216 mPa·s and 31,666 Da before aging to 107.4 mPa·s and 8590 Da after aging at 240 °C for 6.0 h. Meanwhile, the side groups (-SO3− and -COO−) were removed and the unsaturated alkenes were produced due to main chain degradation. In terms of application performance, the fluid loss control ability of the aged product diminished gradually from 22 mL to 196 mL as the aging temperature increased from room temperature to 210 °C. This decline was attributed to a reduction in molecular weight and a decrease in product adsorption capacity caused by the removal of side groups.
Characterization and Rheological Properties of a New Exopolysaccharide Overproduced by Rhizobium sp. L01
Authors: Huang Haolin , Wen Yaolan , Li Zhuangzhuang , Wang Biao , Li Shuang
Published: February 2025

The exopolysaccharides produced by rhizobia play an important role in their biotechnological and bioremediation properties. The characteristics and properties of an exopolysaccharide produced by Rhizobium sp. L01 were investigated. Strain Rhizobium sp. L01 was identified as Rhizobium tropici and produced a high yield of exopolysaccharides (REPS-L01), reaching 22.8 g/L after 63 h of fermentation in a 5 L bioreactor with glucose as the carbon source. REPS-L01 was composed of glucose and galactose in a ratio of 2.95:1, carrying pyruvate, acetate, and succinate groups. REPS-L01 had good shear-thinning properties in aqueous solutions at various concentrations and revealed typical non-crosslinked polymer properties. REPS-L01 revealed thermal stability up to 275 °C. REPS-L01 had the potential to be thicker, being suitable for use under conditions ranging from 4 to 60 °C, pH between 2 and 12, and salt concentrations up to 20,000 mg/L. REPS-L01 showed strong emulsifying activity, particularly with n-hexane; even at concentrations as low as 0.25 wt%, the emulsification index could reach more than 50%. Even more impressively, stable n-hexane emulsion gel was formed with 2 wt% REPS-L01 solution. Rheological studies showed that the solid-like emulsion gel had a high storage modulus, and the SEM studies of the emulsion gel indicated that n-hexane could fill the pores of REPS-L01.
Post-Consumer Recycled PET: A Comprehensive Review of Food and Beverage Packaging Safety in Brazil
Authors: Marcelino Carolina Soares , Gomes Vitor Emanuel de Souza , Marangoni Júnior Luís
Published: February 2025

Polyethylene terephthalate (PET) is widely used in the food and beverage packaging sector due to its chemical and mechanical properties. Although PET is a fossil-based polymer, its recyclability significantly contributes to reducing the environmental impacts caused by excessive plastic consumption. However, the growing demand for post-consumer recycled PET (PET-PCR) food packaging has raised concerns about the efficiency of decontamination processes involved in recycling this material. This review initially addresses PET synthesis processes, highlighting injection stretch blow molding as the predominant technique for packaging production. It then discusses reverse logistics as a strategy to promote sustainability through the recovery of post-consumer packaging, such as plastic bottles. This review examines mechanical and chemical recycling methods used in PET-PCR production, food safety requirements including positive lists of permitted substances, contaminant migration limits, non-intentionally added substances (NIASs), and updated criteria for the National Health Surveillance Agency (ANVISA) of food-grade PET-PCR resins. Finally, the review explores future prospects for using PET-PCR in the food and beverage packaging sector, assessing its environmental impacts and potential technological advancements to enhance its sustainability and safety.