bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of Semi-Automatic Compositional and Microstructural Analysis of Energy Dispersive Spectroscopy (EDS) Maps via a Python-Based Image and Data Processing Framework for Fuel Cell Applications
Authors: Batool Mariah , Godoy Andres O. , Birnbach Martin , Dekel Dario R. , Jankovic Jasna
Published: May 2023

Computer-aided data acquisition, analysis, and interpretation are rapidly gaining traction in numerous facets of research. One of the subsets of this field, image processing, is most often implemented for post-processing material microstructural characterization data to understand better and predict materials’ features, properties, and behaviors at multiple scales. However, to tackle the ambiguity of multi-component materials analysis, spectral data can be used in combination with image processing. The current study introduces a novel Python-based image and data processing method for in-depth analysis of energy dispersive spectroscopy (EDS) elemental maps to analyze multi-component agglomerate size distribution, the average area of each component, and their overlap. The framework developed in this study is applied to examine the interaction of Cerium Oxide (CeOx) and Palladium (Pd) particles in the membrane electrode assembly (MEA) of an Anion-Exchange Membrane Fuel Cell (AEMFC) and to investigate if this approach can be correlated to cell performance. The study also performs a sensitivity analysis of several parameters and their effect on the computed results. The developed framework is a promising method for semi-automatic data processing and can be further advanced towards a fully automatic analysis of similar data types in the field of clean energy materials and broader.
A Novel on Energy Management Strategy with Maximum Exploitation of Renewables and EV Storage in Distribution Networks
Authors: Reddy K. Ramakrishna , Basha C. H. Hussaian , Prashanth V. , Dhanamjayulu C. , Shivashimpiger Sujata , Likhitha R.
Published: May 2023

Electric vehicles (EVs) are widely recognized for their environmentally friendly attributes and superior performance. They offer considerable potential for managing energy in low-voltage distribution networks through the use of vehicle-to-grid (V2G) and grid-to-vehicle (G2V) technologies. This article provides a detailed investigation into the management of energy in distribution networks using a combination of EVs, solar photovoltaic (PV), and diesel generators (DG). The water filling algorithm (WFA) is utilized to distribute the storage of EVs in each energy zone in an optimal manner, thereby achieving load flattening, minimizing energy costs, and reducing grid reliance. A multiobjective genetic algorithm (MOGA) is employed to solve a formulated multiobjective optimization problem for load flattening and voltage regulation, with optimal power transaction (OPT) serving as the decision variable. An adaptive neuro-fuzzy inference system (ANFIS) based EV ranking technique is employed to prioritize EVs based on their ability to provide the required services and determine optimal energy distribution (OED) for different scenarios. This study investigates the impact of OED in several scenarios and examines the influence of ANFIS prioritization on overall EV power availability and cost of charging (CoC). The findings of this study are crucial for developing effective energy management strategies that minimize energy costs and reduce grid reliance.
Nutritional Content Analysis of Crop Residues in Three Agroecologies in East Gojjam Zone
Authors: Desta Alemu Gashe
Published: May 2023

Livestock is important and plentiful in Ethiopia, but its potential is not being used to its full extent due to limited supply and inconsistent feed supply. This has a negative impact on livestock productivity in the tropics, where crop residues are used as livestock feed. The analysis of the nutritional content of feed and the measurement of feed production are crucial for maintaining the optimal balance of annual livestock feed and improving livestock production. However, no research has been conducted on the nutritional composition of the crop residues in the East Gojjam zone. Therefore, this study was conducted to analyze the nutritional content of crop residues in the three agroecologies in Ethiopia’s East Gojjam zone. Ash, dry matter (DM), organic matter (OM), neutral detergent fiber (NDF), acid detergent fiber (ADF), and acid detergent lignin (ADL) were analyzed in the feed samples. According to the findings, the overall mean content of DM, ash, OM, CP, NDF, ADF, and ADL was 92.3, 5.2, 87.1, 4.1, 71, 56.6, and 9.6%, respectively. The faba bean has the highest CP (6.2%), followed by barley straws (5.5%), vetch haulms (5.5%), Eragrostis tef (3.7%), wheat straws (3.2%), and maize stover (2.9%). In general, all crop residues contain CP content below the critical CP level required even for livestock maintenance. The highest NDF was found in barley straw (75.1%), which was followed by wheat straw (72.0%), Eragrostis tef straw (70.2%), vetch haulms (69.3%), maize stover (67.4%), and faba bean haulms (67.2%). All types of crop residues were classified as low-quality feed because they contained more than 65% NDF, which could affect feed intake and livestock production. Therefore, crop residues must be improved using mechanical, biological, and chemical methods in order to increase livestock production in the study areas by increasing feed consumption.
Verifiable Multiparty Delegated Quantum Computation
Authors: Li Qin , Wang Can , Zhu Jiang , Xu Lingling , Sun Zhiwei
Published: May 2023

Multiparty delegated quantum computation (MDQC) allows multiple clients with limited quantum capability to jointly complete a quantum computational task with the aid of an untrusted quantum server. But in existing MDQC protocols, the verifiability that clients should verify whether the server executed the protocol correctly and gave correct results was not handled. Therefore, in this paper, we improve a typical MDQC protocol to enable clients to verify the correctness of computation by inserting trap qubits and develop a novel method to enforce clients to send qubits honestly while avoiding the positions of trap qubits being leaked to the server. The security and verifiability of the improved MDQC protocol are also analyzed. In addition, a specific example of the proposed verifiable MDQC protocol is given and simulated on IBM’s quantum platform.
Two‐Step Unsupervised Approach for Sand‐Dust Image Enhancement
Authors: Gao Guxue , Lai Huicheng , Jia Zhenhong
Published: May 2023

In sand‐dust environments, light is scattered and absorbed, and sand‐dust images thus suffer from severe image degradation problems, such as color shifts, low contrast, and blurred details. To address these problems, we propose a two‐step unsupervised sand‐dust image enhancement algorithm. In the first step, a convenient and competent color correction method is put forward to solve the color shift problem. Considering the wavelength attenuation features of sand‐dust images, a linear stretching and blue channel compensation method is designed, and an adaptive color shift correction factor is developed to remove the color shift. In the second step, to enhance the clarity and details of the images, an unsupervised generative adversarial network is proposed, which does not require pairs of data for training. To reduce detail loss, the detail enhancement branch is designed, and the generator considers to more details through the constructed coarse‐grained and fine‐grained discriminators. The introduced multiscale perceptual loss promotes the image fidelity well. Experiments show that the proposed method achieves better color correction, enhances image details and clarity, has a better subjective effect, and outperforms existing sand‐dust image enhancement methods both quantitatively and qualitatively. Similarly, our method promotes the application capability of the target detection algorithm and also has a good enhancement effect on underwater images and haze images.
Deep Transfer Learning Technique for Multimodal Disease Classification in Plant Images
Authors: Balaji V. , Anushkannan N. K. , Narahari Sujatha Canavoy , Rattan Punam , Verma Devvret , Awasthi Deepak Kumar , Pandian A. Anbarasa , Veeramanickam M. R. M. , Mulat Molla Bayih
Published: May 2023

Rice (Oryza sativa) is India’s major crop. India has the most land dedicated to rice agriculture, which includes both brown and white rice. Rice cultivation creates jobs and contributes significantly to the stability of the gross domestic product (GDP). Recognizing infection or disease using plant images is a hot study topic in agriculture and the modern computer era. This study paper provides an overview of numerous methodologies and analyses key characteristics of various classifiers and strategies used to detect rice illnesses. Papers from the last decade are thoroughly examined, covering studies on several rice plant diseases, and a survey based on essential aspects is presented. The survey aims to differentiate between approaches based on the classifier utilized. The survey provides information on the many strategies used to identify rice plant disease. Furthermore, model for detecting rice disease using enhanced convolutional neural network (CNN) is proposed. Deep neural networks have had a lot of success with picture categorization challenges. We show how deep neural networks may be utilized for plant disease recognition in the context of image classification in this research. Finally, this paper compares the existing approaches based on their accuracy.
Mann Hybrid Deepest-Descent Extragradient Method with Line-Search Process for Hierarchical Variational Inequalities for Countable Nonexpansive Mappings
Authors: Cui Yun-Ling , Ceng Lu-Chuan , Zhang Fang-Fei , He Liang , Yin Jie , Wang Cong-Shan , Hu Hui-Ying
Published: May 2023

In real Hilbert spaces, let the CFPP indicate a common fixed-point problem of asymptotically nonexpansive operator and countably many nonexpansive operators, and suppose that the HVI and VIP represent a hierarchical variational inequality and a variational inequality problem, respectively. We put forward Mann hybrid deepest-descent extragradient approach for solving the HVI with the CFPP and VIP constraints. The proposed algorithms are on the basis of Mann’s iterative technique, viscosity approximation method, subgradient extragradient rule with linear-search process, and hybrid deepest-descent rule. Under suitable restrictions, it is shown that the sequences constructed by the algorithms converge strongly to a solution of the HVI with the CFPP and VIP constraints.
Students’ Perception toward Teaching Strategies of Native and Nonnative English-Speaking Teachers: A Case Study in Indonesia
Authors: Rasyid Fathor , Ibna Seraj Prodhan Mahbub , Ghofur Abd. , Asrifan Andi
Published: May 2023

This study aimed to investigate the students’ insights toward native English-speaking teachers’ (NESTs) and nonnative English-speaking teachers’ (NNEST) teaching strategies and to examine the reasons for their insights. Fifty-eight English students, 30 females, and 20 males, with prior experience of learning from both NESTs and NNESTs in East Java, Indonesia, participated in the study. They were purposively selected. A set of questionnaires was used to elicit quantitative data on students’ perceptions and focus group discussion was used to elicit qualitative data on the underlying reasons for their perceptions. Quantitative data were analyzed with descriptive and inferential statistics, whereas qualitative data were analyzed based on themes. The finding revealed that the students perceived NESTs slightly better than NNESTs, with a grand mean of 9.92 and 3.74. Another finding indicates that the perception percentage for NESTs is 75%, while NNESTs achieved 63.8%. However, when the grand means of both groups are tested statistically using a correlated sample t-test, it reveals that there is no significant mean difference (p<0.05). This means that the mean difference of 3.92 and 3.74 occurred by chance only, and it is not considered different statistically. This suggests that the students perceived NESTs and NNESTs the same. The qualitative data were classified into six themes: explanation, class interaction, teaching strategy, improvization, and ideal teachers. The data indicated that students have more or less similar reasons for these themes. Eventually, the results of qualitative and quantitative data analysis suggest that NESTs and NNESTs are not two distinct groups, one necessarily better or more qualified to be teachers than the other.
Connection Number- Based Topological Indices of Cartesian Product of Graphs
Authors: Arshad Aiman , Sattar Aqsa , Javaid Muhammad , Abebe Ashebo Mamo
Published: May 2023

The area of graph theory (GT) is rapidly expanding and playing a significant role in cheminformatics, mostly in mathematics and chemistry to develop different physicochemical, chemical structure, and their properties. The manipulation and study of chemical graphical details are made feasible by using numerical structure invariant. Investigating these chemical characteristics of topological indices (TIs) is made possible by the discipline of mathematical chemistry. In this article, we study with the Cartesian product of complete graphs, with path graphs, and find their general result of connection number (CN)-based TIs, namely, first connection- based Zagreb index (1st CBZI), second connection- based Zagreb index (2nd CBZI), and third CBZI (3rd CBZI) and then modified first connection- based Zagreb index (CBZI) and second and third modified CBZIs. We also express the general results of first multiplicative CBZI, second multiplicative CBZI, and third and fourth multiplicative CBZI, of two special types of graphs, namely, complete graphs and path graphs. More precisely, we arrange the graphical and numerical analysis of our calculated expressions for both of Cartesian product with each other.
Adsorption Characteristics and Electrochemical Behaviors of Methyl Blue onto Magnetic MgxCoyZn(1-x-y)Fe2O4 Nanoparticles
Authors: Lv Zhixiang , Yang Xin , Han Jihong , Wang Yingyao , Zou Jiao , Yang Anqi , Zhang Haoda , He Nan
Published: May 2023

Magnetic MgxCoyZn(1-x-y)Fe2O4 nanoparticles were successfully prepared by the rapid combustion approach, and SEM, XRD, VSM, EDX, and FTIR techniques were applied for their characterization. The influence of the element ratios (Mg2+, Co2+, and Zn2+) in magnetic MgxCoyZn(1-x-y)Fe2O4 nanoparticles on their properties was explored. To acquire a larger specific surface area for better adsorption of methyl blue (MB), magnetic Mg0.4Co0.5Zn0.1Fe2O4 nanoparticles calcined at 400°C for 2 h with 25 mL anhydrous ethanol were selected, and their average particle size and the saturation magnetization were about 81.3 nm and 13.5 emu·g-1, respectively. Adsorption kinetics models and adsorption isotherm models were applied to research the adsorption characteristics of MB onto magnetic Mg0.4Co0.5Zn0.1Fe2O4 nanoparticles. The pseudo-second-order kinetics model ([Formula: see text]) and Temkin isotherm model ([Formula: see text]) were the most consistent with the data, indicating that the adsorption was the chemical multilayer adsorption mechanism, and the process was an exothermic reaction. The E of the Dubinin-Radushkevich (D-R) isotherm model was 0.2347 KJ·mol-1, indicating the adsorption involved physical adsorption besides chemical adsorption. The [Formula: see text] and [Formula: see text] ([Formula: see text] KJ·mol-1) of the adsorption process of MB adsorbed onto magnetic Mg0.4Co0.5Zn0.1Fe2O4 nanoparticles measured through the thermodynamic experiment were both less than 0, which proved that the process was a spontaneous exothermic reaction. The adsorption capacity of MB onto magnetic Mg0.4Co0.5Zn0.1Fe2O4 nanoparticles increased with the pH of MB solution increasing from 2 to 4 at room temperature, and it had no significant change when the pH of MB solution was 4-12, while the relative removal rate was 98.75% of the first one after 2 cycles. The electrochemical impedance spectroscopy (EIS) and the cyclic voltammetry (CV) data further demonstrated that MB was adsorbed onto magnetic Mg0.4Co0.5Zn0.1Fe2O4 nanoparticles.
Statistical Analysis of Train Operation and Passenger Distribution Based on Real Records: A Case Study of Wuhan-Guangzhou HSR
Authors: Li Jie , Peng Qiyuan , Wen Chao
Published: May 2023

This paper summarizes the results of an effort aimed at improving train operation schedules on Wuhan-Guangzhou high-speed railway (WG-HSR). The real-record train operation and passenger tickets-booking records of WG-HSR are used for statistical analysis on the train service quality and passenger distribution. More specifically, the train service frequency and interval time at each station are analyzed. Based on this, the temporal and spatial distribution of capacity utilization in each section are investigated. In order to get a holistic view of passenger flow characteristics, the passenger volume during different time periods and between several origin and destination (OD) pairs are investigated to characterize travellers’ spatial-temporal preferences. The passenger distributions on some long-distance trains are shown to get the number and proportion of cross-line passengers travelling on the WG-HSR. Moreover, for a better understanding of the seat capacity utilization of trains, the load rates of trains in various sections and time periods are investigated. Specifically, the relationship between the average load rate of trains and trains’ running distance is explored, finding that the longer the non-cross-line train travels is, the higher the average load rate is. This study provides insightful findings that help understanding HSR operation and conducting further research.
Periodontal Health and Disease in the Context of Systemic Diseases
Authors: Isola Gaetano , Santonocito Simona , Lupi Saturnino Marco , Polizzi Alessandro , Sclafani Rossana , Patini Romeo , Marchetti Enrico
Published: May 2023

During recent years, considerable progress has been made in understanding the etiopathogenesis of periodontitis in its various forms and their interactions with the host. Furthermore, a number of reports have highlighted the importance of oral health and disease in systemic conditions, especially cardiovascular diseases and diabetes. In this regard, research has attempted to explain the role of periodontitis in promoting alteration in distant sites and organs. Recently, DNA sequencing studies have revealed how oral infections can occur in distant sites such as the colon, reproductive tissues, metabolic diseases, and atheromas. The objective of this review is to describe and update the emerging evidence and knowledge regarding the association between periodontitis and systemic disease and to analyse the evidence that has reported periodontitis as a risk factor for the development of various forms of systemic diseases in order to provide a better understanding of the possible shared etiopathogenetic pathways between periodontitis and the different forms of systemic diseases.
Supplementary Data from FPR2 Shapes an Immune-Excluded Pancreatic Tumor Microenvironment and Drives T-cell Exhaustion in a Sex-Dependent Manner
Authors: He Fei , Tay Apple H.M. , Calandigary Ahmed , Malki Enana , Suzuki Sayaka , Liu Tianjie , Wang Qi , Fernández-Moro Carlos , Kaisso Marina , Olofsson-Sahl Peter , Melssen Marit , Sze Siu Kwan , Björnstedt Mikael , Löhr Matthias J. , Karlsson Mikael C.I. , Heuchel Rainer , Sarhan Dhifaf
Published: May 2023

Figure S1-S8, and Table S1-S2
Supplementary Figures from Microenvironment-Driven Dynamic Chromatin Changes in Glioblastoma Recapitulate Early Neural Development at Single-Cell Resolution
Authors: Pine Allison R. , Cirigliano Stefano M. , Singhania Richa , Nicholson James , da Silva Bárbara , Leslie Christina S. , Fine Howard A.
Published: May 2023

Supplementary Figures (Figures S1-S5)
Supplementary Figures from NAT10 Drives Cisplatin Chemoresistance by Enhancing ac4C-Associated DNA Repair in Bladder Cancer
Authors: Xie Ruihui , Cheng Liang , Huang Ming , Huang Lin , Chen Ziyue , Zhang Qiang , Li Hong , Lu Junlin , Wang Hongjin , Zhou Qianghua , Huang Jian , Chen Xu , Lin Tianxin
Published: May 2023

Supplementary Figures
Figure S5 from BRCA2 Germline Mutations Identify Gastric Cancers Responsive to PARP Inhibitors
Authors: Petrelli Annalisa , Rizzolio Sabrina , Pietrantonio Filippo , Bellomo Sara E. , Benelli Matteo , De Cecco Loris , Romagnoli Dario , Berrino Enrico , Orrù Claudia , Ribisi Salvatore , Moya-Rull Daniel , Migliore Cristina , Conticelli Daniela , Maina Irene M. , Puliga Elisabetta , Serra Violeta , Pellegrino Benedetta , Llop-Guevara Alba , Musolino Antonino , Siena Salvatore , Sartore-Bianchi Andrea , Prisciandaro Michele , Morano Federica , Antista Maria , Fumagalli Uberto , De Manzoni Giovanni , Degiuli Maurizio , Baiocchi Gian Luca , Amisano Marco F. , Ferrero Alessandro , Marchiò Caterina , Corso Simona , Giordano Silvia
Published: May 2023

Microsatellite instability (MSI) evaluation in the GTR0264 and GTR0324 tumors.
Figure S2 from BRCA2 Germline Mutations Identify Gastric Cancers Responsive to PARP Inhibitors
Authors: Petrelli Annalisa , Rizzolio Sabrina , Pietrantonio Filippo , Bellomo Sara E. , Benelli Matteo , De Cecco Loris , Romagnoli Dario , Berrino Enrico , Orrù Claudia , Ribisi Salvatore , Moya-Rull Daniel , Migliore Cristina , Conticelli Daniela , Maina Irene M. , Puliga Elisabetta , Serra Violeta , Pellegrino Benedetta , Llop-Guevara Alba , Musolino Antonino , Siena Salvatore , Sartore-Bianchi Andrea , Prisciandaro Michele , Morano Federica , Antista Maria , Fumagalli Uberto , De Manzoni Giovanni , Degiuli Maurizio , Baiocchi Gian Luca , Amisano Marco F. , Ferrero Alessandro , Marchiò Caterina , Corso Simona , Giordano Silvia
Published: May 2023

BRCA2 LOF mutations in the GEA PDX models used in pre-clinical trials.
Supplementary Fig. S6 from Single-Cell Spatial Analysis Identifies Regulators of Brain Tumor–Initiating Cells
Authors: Mirzaei Reza , D'Mello Charlotte , Liu Marina , Nikolic Ana , Kumar Mehul , Visser Frank , Bose Pinaki , Gallo Marco , Yong V. Wee
Published: May 2023

Additional analysis of human GBM specimens for biglycan and BTICs.
Supplementary Fig. S4 from Single-Cell Spatial Analysis Identifies Regulators of Brain Tumor–Initiating Cells
Authors: Mirzaei Reza , D'Mello Charlotte , Liu Marina , Nikolic Ana , Kumar Mehul , Visser Frank , Bose Pinaki , Gallo Marco , Yong V. Wee
Published: May 2023

Additional analysis showing clusters of scRNA-seq analysis with more stemness profiles.
Supplementary Fig. S10 from Single-Cell Spatial Analysis Identifies Regulators of Brain Tumor–Initiating Cells
Authors: Mirzaei Reza , D'Mello Charlotte , Liu Marina , Nikolic Ana , Kumar Mehul , Visser Frank , Bose Pinaki , Gallo Marco , Yong V. Wee
Published: May 2023

Survival analysis of GBM patients in public databases.
Data from BRCA2 Germline Mutations Identify Gastric Cancers Responsive to PARP Inhibitors
Authors: Petrelli Annalisa , Rizzolio Sabrina , Pietrantonio Filippo , Bellomo Sara E. , Benelli Matteo , De Cecco Loris , Romagnoli Dario , Berrino Enrico , Orrù Claudia , Ribisi Salvatore , Moya-Rull Daniel , Migliore Cristina , Conticelli Daniela , Maina Irene M. , Puliga Elisabetta , Serra Violeta , Pellegrino Benedetta , Llop-Guevara Alba , Musolino Antonino , Siena Salvatore , Sartore-Bianchi Andrea , Prisciandaro Michele , Morano Federica , Antista Maria , Fumagalli Uberto , De Manzoni Giovanni , Degiuli Maurizio , Baiocchi Gian Luca , Amisano Marco F. , Ferrero Alessandro , Marchiò Caterina , Corso Simona , Giordano Silvia
Published: May 2023

Abstract
Despite negative results of clinical trials conducted on the overall population of patients with gastric cancer, PARP inhibitor (PARPi) therapeutic strategy still might represent a window of opportunity for a subpopulation of patients with gastric cancer. An estimated 7% to 12% of gastric cancers exhibit a mutational signature associated with homologous recombination (HR) failure, suggesting that these patients could potentially benefit from PARPis. To analyze responsiveness of gastric cancer to PARPi, we exploited a gastroesophageal adenocarcinoma (GEA) platform of patient-derived xenografts (PDX) and PDX-derived primary cells and selected 10 PDXs with loss-of-function mutations in HR pathway genes. Cell viability assays and preclinical trials showed that olaparib treatment was effective in PDXs harboring BRCA2 germline mutations and somatic inactivation of the second allele. Olaparib responsive tumors were sensitive to oxaliplatin as well. Evaluation of HR deficiency (HRD) and mutational signatures efficiently stratified responder and nonresponder PDXs. A retrospective analysis on 57 patients with GEA showed that BRCA2 inactivating variants were associated with longer progression-free survival upon platinum-based regimens. Five of 7 patients with BRCA2 germline mutations carried the p.K3326* variant, classified as “benign.” However, familial history of cancer, the absence of RAD51 foci in tumor cells, and a high HRD score suggest a deleterious effect of this mutation in gastric cancer. In conclusion, PARPis could represent an effective therapeutic option for BRCA2-mutated and/or high HRD score patients with GEA, including patients with familial intestinal gastric cancer.
Significance:
PARP inhibition is a potential strategy for treating patients with gastric cancer with mutated BRCA2 or homologous repair deficiency, including patients with familial intestinal gastric cancer, for whom BRCA2 germline testing should be recommended.
Supplementary Table S1 from Randomized Phase II Cabazitaxel Dose Individualization and Neutropenia Prevention Trial in Patients with Metastatic Castration-Resistant Prostate Cancer
Authors: Omlin Aurelius , Cathomas Richard , von Amsberg Gunhild , Reuter Christoph , Feyerabend Susan , Loidl Wolfgang , Boegemann Martin , Lorch Anja , Heidenreich Axel , Tsaur Igor , Larcher-Senn Julian , Buck Stefan A.J. , Mathijssen Ron H.J. , Jaehde Ulrich , Gillessen Silke , Joerger Markus
Published: May 2023

Representativeness of Study Participants
Figure S4 from Immunologic Predictors for Clinical Responses during Immune Checkpoint Blockade in Patients with Myelodysplastic Syndromes
Authors: Lee Sung-Eun , Wang Feng , Grefe Maison , Trujillo-Ocampo Abel , Ruiz-Vasquez Wilfredo , Takahashi Koichi , Abbas Hussein A. , Borges Pamella , Antunes Dinler Amaral , Al-Atrash Gheath , Daver Naval , Molldrem Jeffrey J. , Futreal Andrew , Garcia-Manero Guillermo , Im Jin S.
Published: May 2023

Figure S4. Changes in T cell subsets at the time of the best responses
Supplementary Table S3 from Randomized Trial of Tepotinib Plus Gefitinib versus Chemotherapy in EGFR-Mutant NSCLC with EGFR Inhibitor Resistance Due to MET Amplification: INSIGHT Final Analysis
Authors: Liam Chong Kin , Ahmad Azura Rozila , Hsia Te-Chun , Zhou Jianying , Kim Dong-Wan , Soo Ross Andrew , Cheng Ying , Lu Shun , Shin Sang Won , Yang James Chih-Hsin , Zhang Yiping , Zhao Jun , Berghoff Karin , Bruns Rolf , Johne Andreas , Wu Yi-Long
Published: May 2023

Demographic data in the patient subgroup with MET overexpression
Figure S3 from Immunologic Predictors for Clinical Responses during Immune Checkpoint Blockade in Patients with Myelodysplastic Syndromes
Authors: Lee Sung-Eun , Wang Feng , Grefe Maison , Trujillo-Ocampo Abel , Ruiz-Vasquez Wilfredo , Takahashi Koichi , Abbas Hussein A. , Borges Pamella , Antunes Dinler Amaral , Al-Atrash Gheath , Daver Naval , Molldrem Jeffrey J. , Futreal Andrew , Garcia-Manero Guillermo , Im Jin S.
Published: May 2023

Figure S3. Flow-cytometry gating strategy. CD, cluster of differentiation, Treg, regulatory T cell
Supplementary Figure S3 from Randomized Trial of Tepotinib Plus Gefitinib versus Chemotherapy in EGFR-Mutant NSCLC with EGFR Inhibitor Resistance Due to MET Amplification: INSIGHT Final Analysis
Authors: Liam Chong Kin , Ahmad Azura Rozila , Hsia Te-Chun , Zhou Jianying , Kim Dong-Wan , Soo Ross Andrew , Cheng Ying , Lu Shun , Shin Sang Won , Yang James Chih-Hsin , Zhang Yiping , Zhao Jun , Berghoff Karin , Bruns Rolf , Johne Andreas , Wu Yi-Long
Published: May 2023

Outcomes of treatment for the patient subgroup with MET overexpression
Table S2 from PSMA-positive Circulating Tumor Cell Detection and Outcomes with Abiraterone or Enzalutamide Treatment in Men with Metastatic Castrate-resistant Prostate Cancer
Authors: Gupta Santosh , Halabi Susan , Yang Qian , Roy Akash , Tubbs Alisa , Gore Yamini , George Daniel J. , Nanus David M. , Antonarakis Emmanuel S. , Danila Daniel C. , Szmulewitz Russell Z. , Wenstrup Richard , Armstrong Andrew J.
Published: May 2023

The prevalence of PSMA and Cell Search CTC positivity (≥5) at baseline and progression, as well as the observed median and optimal cutoffs for PSMA CTC.
Table S1 from PSMA-positive Circulating Tumor Cell Detection and Outcomes with Abiraterone or Enzalutamide Treatment in Men with Metastatic Castrate-resistant Prostate Cancer
Authors: Gupta Santosh , Halabi Susan , Yang Qian , Roy Akash , Tubbs Alisa , Gore Yamini , George Daniel J. , Nanus David M. , Antonarakis Emmanuel S. , Danila Daniel C. , Szmulewitz Russell Z. , Wenstrup Richard , Armstrong Andrew J.
Published: May 2023

Comparison of CTC enumeration and PSMA+ CTC expression in 57 baseline and paired progression samples from the PROPHECY study. All the CTC parameters (mean, median, and range) were represent in CTC/mL.
Table S1 from Quiescent Ovarian Cancer Cells Secrete Follistatin to Induce Chemotherapy Resistance in Surrounding Cells in Response to Chemotherapy
Authors: Cole Alexander J. , Panesso-Gómez Santiago , Shah Jaynish S. , Ebai Tonge , Jiang Qi , Gumusoglu-Acar Ece , Bello Maya G. , Vlad Anda , Modugno Francesmary , Edwards Robert P. , Buckanovich Ronald J.
Published: May 2023

Supplementary Table 1. Primers used in this study.
Figure S3 from PSMA-positive Circulating Tumor Cell Detection and Outcomes with Abiraterone or Enzalutamide Treatment in Men with Metastatic Castrate-resistant Prostate Cancer
Authors: Gupta Santosh , Halabi Susan , Yang Qian , Roy Akash , Tubbs Alisa , Gore Yamini , George Daniel J. , Nanus David M. , Antonarakis Emmanuel S. , Danila Daniel C. , Szmulewitz Russell Z. , Wenstrup Richard , Armstrong Andrew J.
Published: May 2023

Waterfall plots depicting A) the proportion of men achieving a 50% or greater PSA decline from baseline, confirmed with a subsequent value (confirmed PSA50) and B) best overall objective radiographic responses among response evaluable subjects at baseline, by RECIST 1.1 criteria.
Figure S3 from Quiescent Ovarian Cancer Cells Secrete Follistatin to Induce Chemotherapy Resistance in Surrounding Cells in Response to Chemotherapy
Authors: Cole Alexander J. , Panesso-Gómez Santiago , Shah Jaynish S. , Ebai Tonge , Jiang Qi , Gumusoglu-Acar Ece , Bello Maya G. , Vlad Anda , Modugno Francesmary , Edwards Robert P. , Buckanovich Ronald J.
Published: May 2023

Supplementary Figure 3. The effect of FST or taxol treatment ovarian cancer proliferation and FST secretion, and the ability of a FST neutralization antibody to inhibit FST's effect on proliferation. A. PT340 and PT412 cells were treated with 200 ng/mL FST for 72h before cell counts were performed. B (i) IHC images and (ii) FST+ cell bodies in OVSAHO tumor xenografts treated with or without taxol and stained with both Ki67 (Forangi Blue) and FST (Fast Red). C. Titer of the ability of the anti-FST neutralizing antibody to inhibit the effect of FST on cell growth.
Figure S2 from Quiescent Ovarian Cancer Cells Secrete Follistatin to Induce Chemotherapy Resistance in Surrounding Cells in Response to Chemotherapy
Authors: Cole Alexander J. , Panesso-Gómez Santiago , Shah Jaynish S. , Ebai Tonge , Jiang Qi , Gumusoglu-Acar Ece , Bello Maya G. , Vlad Anda , Modugno Francesmary , Edwards Robert P. , Buckanovich Ronald J.
Published: May 2023

Supplementary Figure 2. RNA-seq analysis. A. Schematic of the RNA-seq experimental design. B. Waterfall plot of RNA-seq data showing the 141 genes which were DE at all time points compared to luciferase control in HEY1 and SKOV3 cell lines. C. Unsupervised clustering analysis of the 141 DE genes. D. Box plots demonstrating MYC expression following NFATC4 activation. E. STRING analysis of the 141 DE genes.
Supplementary Table S3 from Durvalumab plus Cetuximab in Patients with Recurrent or Metastatic Head and Neck Squamous Cell Carcinoma: An Open-label, Nonrandomized, Phase II Clinical Trial
Authors: Gulati Shuchi , Crist McKenzie , Riaz Muhammed Kashif , Takiar Vinita , Lehn Maria , Monroe Ilaina , Palackdharry Sarah , Kurtzweil Nicky , Jandarov Roman , Harun Nusrat , Wise-Draper Trisha M.
Published: May 2023

Representativeness of Study Participants
Supplementary Table S1 from Durvalumab plus Cetuximab in Patients with Recurrent or Metastatic Head and Neck Squamous Cell Carcinoma: An Open-label, Nonrandomized, Phase II Clinical Trial
Authors: Gulati Shuchi , Crist McKenzie , Riaz Muhammed Kashif , Takiar Vinita , Lehn Maria , Monroe Ilaina , Palackdharry Sarah , Kurtzweil Nicky , Jandarov Roman , Harun Nusrat , Wise-Draper Trisha M.
Published: May 2023

All adverse events on trial
Supplementary Figure S4 from Safety and Efficacy of Axicabtagene Ciloleucel versus Standard of Care in Patients 65 Years of Age or Older with Relapsed/Refractory Large B-Cell Lymphoma
Authors: Westin Jason R. , Locke Frederick L. , Dickinson Michael , Ghobadi Armin , Elsawy Mahmoud , van Meerten Tom , Miklos David B. , Ulrickson Matthew L. , Perales Miguel-Angel , Farooq Umar , Wannesson Luciano , Leslie Lori , Kersten Marie José , Jacobson Caron A. , Pagel John M. , Wulf Gerald , Johnston Patrick , Rapoport Aaron P. , Du Linqiu , Vardhanabhuti Saran , Filosto Simone , Shah Jina , Snider Julia T. , Cheng Paul , To Christina , Oluwole Olalekan O. , Sureda Anna
Published: May 2023

Mixed model with repeated measures for change from baseline for prespecified patient-reported outcomes endpoints in patients ≥65 years
Supplementary Figure S4 from Durvalumab plus Cetuximab in Patients with Recurrent or Metastatic Head and Neck Squamous Cell Carcinoma: An Open-label, Nonrandomized, Phase II Clinical Trial
Authors: Gulati Shuchi , Crist McKenzie , Riaz Muhammed Kashif , Takiar Vinita , Lehn Maria , Monroe Ilaina , Palackdharry Sarah , Kurtzweil Nicky , Jandarov Roman , Harun Nusrat , Wise-Draper Trisha M.
Published: May 2023

Supplementary Figure 4. Comparison of outcomes between patients treated with prior systemic therapies vs. not A) Progression-free survival and B) Overall survival comparison
Supplementary Figure S3 from Safety and Efficacy of Axicabtagene Ciloleucel versus Standard of Care in Patients 65 Years of Age or Older with Relapsed/Refractory Large B-Cell Lymphoma
Authors: Westin Jason R. , Locke Frederick L. , Dickinson Michael , Ghobadi Armin , Elsawy Mahmoud , van Meerten Tom , Miklos David B. , Ulrickson Matthew L. , Perales Miguel-Angel , Farooq Umar , Wannesson Luciano , Leslie Lori , Kersten Marie José , Jacobson Caron A. , Pagel John M. , Wulf Gerald , Johnston Patrick , Rapoport Aaron P. , Du Linqiu , Vardhanabhuti Saran , Filosto Simone , Shah Jina , Snider Julia T. , Cheng Paul , To Christina , Oluwole Olalekan O. , Sureda Anna
Published: May 2023

Sensitivity analysis of interim overall survival in patients ≥65 years
Supplementary Information 1 from The Oncolytic Adenovirus XVir-N-31 Joins Forces with CDK4/6 Inhibition Augmenting Innate and Adaptive Antitumor Immunity in Ewing Sarcoma
Authors: Schober Sebastian Johannes , Schoening Caroline , Eck Jennifer , Middendorf Charlotte , Lutsch Julia , Knoch Pia , von Ofen Anna Josefine , Gassmann Hendrik , Thiede Melanie , Hauer Julia , Kolk Andreas , Mantwill Klaus , Gschwend Jürgen E. , Burdach Stefan E.G. , Nawroth Roman , Thiel Uwe , Holm Per Sonne
Published: May 2023

Primers, antibodies and dyes
Figure S2 from The Oncolytic Adenovirus XVir-N-31 Joins Forces with CDK4/6 Inhibition Augmenting Innate and Adaptive Antitumor Immunity in Ewing Sarcoma
Authors: Schober Sebastian Johannes , Schoening Caroline , Eck Jennifer , Middendorf Charlotte , Lutsch Julia , Knoch Pia , von Ofen Anna Josefine , Gassmann Hendrik , Thiede Melanie , Hauer Julia , Kolk Andreas , Mantwill Klaus , Gschwend Jürgen E. , Burdach Stefan E.G. , Nawroth Roman , Thiel Uwe , Holm Per Sonne
Published: May 2023

Supplemental Figure 2: Combination of XVir-N-31 (XVir) and CDK4/6 inhibitor LEE011 (LEE) increases viral replication and immunogenicity of xenografted EwS tumors in vivo.
TABLE 1 from Development of an Anti-canine PD-L1 Antibody and Caninized PD-L1 Mouse Model as Translational Research Tools for the Study of Immunotherapy in Humans
Authors: Oh Wonkyung , Kim Alyssa Min Jung , Dhawan Deepika , Kirkham Perry M. , Ostafe Raluca , Franco Jackeline , Aryal Uma K. , Carnahan Robert H. , Patsekin Valery , Robinson J. Paul , Knapp Deborah W. , Lim Seung-Oe
Published: May 2023

Summary of potential adverse events with the initial cPD-L1 antibody administration to laboratory dogs
FIGURE 5 from Development of an Anti-canine PD-L1 Antibody and Caninized PD-L1 Mouse Model as Translational Research Tools for the Study of Immunotherapy in Humans
Authors: Oh Wonkyung , Kim Alyssa Min Jung , Dhawan Deepika , Kirkham Perry M. , Ostafe Raluca , Franco Jackeline , Aryal Uma K. , Carnahan Robert H. , Patsekin Valery , Robinson J. Paul , Knapp Deborah W. , Lim Seung-Oe
Published: May 2023

Evaluation of the caninized cPD-L1 chimeric antibody. A, 12C antibody binding on the BT549cPD-L1 cells. B, Flow cytometric analysis of the 12C chimeric antibody on the BT549cPD-L1 cells. cIgG serves as a negative control. C, 12C chimeric antibody binding to cPD-L1 and cPD-L2. D, Binding affinity (KD) analysis of 12C chimeric antibody by Octet. E, EC50 of 12C chimeric antibody, 12C10E4. EC50 = 0.419 μg/mL. The bound cPD-1 protein was quantified by measuring green fluorescence at the IncuCyte S3. F and G, Canine IO Panel (NanoString) analyses were used to query changes in gene expression upon activation of cPBMCs from three healthy pet dogs. The RNA from the resting and activated PBMCs was used for NanoString work. The Canine IO panel was used to query the changes in approximately 700 genes. Groupwise analyses were conducted using “Rosalind.” There were 65 genes that were differentially expressed when comparing control PBMCs with activated PBMCs (P < 0.05, FC > 1.5) including 30 upregulated and 35 downregulated genes. In the heatmap, each column consists of data from one sample. IFNγ (H) and TNFα (I) concentrations were analyzed in the activated canine PBMCs. J and K, Flow cytometric analysis of cPD-L1 protein expression on the K9TCC or the nuclear-restricted RFP-expressing K9TCC (K9TCCnRFP) cells using the 12C chimeric antibody. The endogenous PD-L1 expression was stimulated by 50 ng/mL canine IFNγ for 12 hours. cIgG served as a negative control. L, The quantitative RT-PCR analysis of cPD-L1 (CD274) mRNA expression in the K9TCC or K9TCCnRFP cells. M, The 12C chimeric antibody enhances the tumor cell killing. Canine bladder cancer, K9TCC cells were cocultured with cPBMCs that were activated with CD3 antibody (100 ng/mL) and IL2 (10 ng/mL) at a ratio of 1 tumor cell: 15 cPBMCs. The live tumor cell count at 72 hours is shown in the bar graph. N, IFNγ concentrations were analyzed in the medium from the coculture of the K9TCC cells and activated cPBMCs with/without the 12C chimeric antibody treatment.
FIGURE 4 from Development of an Anti-canine PD-L1 Antibody and Caninized PD-L1 Mouse Model as Translational Research Tools for the Study of Immunotherapy in Humans
Authors: Oh Wonkyung , Kim Alyssa Min Jung , Dhawan Deepika , Kirkham Perry M. , Ostafe Raluca , Franco Jackeline , Aryal Uma K. , Carnahan Robert H. , Patsekin Valery , Robinson J. Paul , Knapp Deborah W. , Lim Seung-Oe
Published: May 2023

The quality attributes of the purified anti-cPD-L1, 1210E4 (12C) chimeric antibody. A, Canine PD-L1, 12C10E4 chimeric antibody expression construct, pTRIOZ-cIgG2-cPD-L1 12C10E4. B, SDS-PAGE analysis of 12C chimeric antibody purity under nonreducing and reducing (2-mercaptoethanol) conditions. HC, heavy chain; LC, light chain; SM, protein size marker. C,IEF analysis of 12C chimeric antibody. Standard, pI standard. D, Peptide mapping analysis. The peptide mapping of cPD-L1 12C chimeric antibody. The 12C chimeric antibody was enzymatically digested with trypsin on S-trap micro columns from Protifi after reduction and alkylation. Peptides were then separated and analyzed by RP-LC/MS-MS. The resultant mass spectrometric data were analyzed using the PEAK PTM workflow in the PEAKS X PRO Studio 10.6 software package from Bioinformatics solutions Inc. to map the detected MS1 and MS2 ions to the amino acid sequence of antibody. The sequence coverage of heavy and light chains was 100% (453 of 453 amino acids) and 98.2 (223 of 227 amino acids), respectively. E,SEC analysis of 12C chimeric antibody. Standard, SEC standard. F, MALDI-MS profiling of permethylated N-glycans released from PNGase F-treated 12C chimeric antibody. The masses of indicated glycan species represent the [M + Na+] values.
FIGURE 3 from Development of an Anti-canine PD-L1 Antibody and Caninized PD-L1 Mouse Model as Translational Research Tools for the Study of Immunotherapy in Humans
Authors: Oh Wonkyung , Kim Alyssa Min Jung , Dhawan Deepika , Kirkham Perry M. , Ostafe Raluca , Franco Jackeline , Aryal Uma K. , Carnahan Robert H. , Patsekin Valery , Robinson J. Paul , Knapp Deborah W. , Lim Seung-Oe
Published: May 2023

cPD-L1 antibodies enhance antitumor immunity in the caninized PD-L1 syngeneic mouse model. A, Knock-in strategy of the caninized PD-L1 mice (c57BL/c background). B, Validation of cPD-L1 protein expression in the MB49cPD-L1 cells. Flow cytometric analysis of membrane located mPD-L1 and cPD-L1 protein in MB49 cells expressing cPD-L1 (MB49cPD-L1) or MB49 parental cells. C, Immunofluorescence staining and protein expression pattern of mPD-L1 and cPD-L1 in MB49 or MB49cPD-L1 tumor masses from the caninized PD-L1 mice. DAPI, nuclear counterstaining. Scale bar, 100 μm. D, Interaction of cPD-1 or mPD-1 protein with cPD-L1 or mPD-L1 protein with or without cPD-L1 antibody, 12C. His-tagged canine or mPD-L1 protein was immobilized on the Ni-NTA 96-well plate, and HRP-conjugated anti-human IgG Fc-specific secondary with mPD-1-hFc or cPD-1-hFc protein was added. OD450 was measured to quantify the amount of bound PD-1 protein. E, Binding of cPD-L1 antibodies, 12C and 3C, with human PD-L1 (hPD-L1), mPD-L1, and cPD-L1 proteins. His-tagged human PD-L1, mPD-L1, or cPD-L1 protein was immobilized on the Ni-NTA 96-well plate, and anti-cPD-L1 antibodies, 12C or 3C, with HRP-conjugated anti-canine IgG-specific secondary was added. OD450 was measured to quantify the amount of bound PD-L1 antibodies. Ab, antibody. F, Tumor growth of MB49cPD-L1 in the caninized PD-L1 mice treated with cPD-L1 antibody, 12C or 3C. The IgG isotype of 12C and 3C antibodies is mouse IgG1 which is equivalent to human IgG4. Tumors were measured at the indicated timepoints (n = 8 per group). At the endpoint, the tumors were dissected. G–I, Immunofluorescence staining, and protein expression pattern of CD8 and granzyme B in MB49 tumor masses from IgG-, 12C-, or 3C-treated mice. DAPI, nuclear counterstaining. Scale bar, 100 μm. Representative images of immunostaining of CD8 and granzyme B in the MB49 tumor mass (G). CD8 (H) and granzyme B (I) were quantified using Gen5 software (BioTek). n = 10. Treatment with the PD-L1 antibody did not affect kidney function (serum creatinine; J) or liver enzyme activity (ALT; K), measured in blood collected at the end of the experiment. ALT, alanine aminotransferase.
Para além do acesso ao medicamento: papel do SUS e perfil da assistência em HIV no Brasil
Authors: Alves Ana Maroso , Santos Angélica Carreira dos , Kumow Aline , Sato Ana Paula Sayuri , Helena Ernani Tiaraju de Santa , Nemes Maria Ines Battistella
Published: May 2023

OBJETIVO: Estimar a composição público-privada da assistência em HIV no Brasil e o perfil organizacional da extensa rede de serviços públicos. MÉTODOS: Foram utilizados dados da Coorte Qualiaids-BR, que reúne dados dos sistemas nacionais de informações clínicas e laboratoriais de pessoas com 15 anos ou mais com primeira dispensação de terapia antirretroviral, entre 2015–2018, e informações dos serviços do SUS de acompanhamento clínico-laboratorial do HIV, produzidas pelo inquérito Qualiaids. O sistema de acompanhamento foi definido pelo número de exames de carga viral solicitados por algum serviço do SUS: acompanhamento no sistema privado – nenhum registro; acompanhamento no SUS – dois ou mais registros; acompanhamento indefinido – um registro. Os serviços do SUS foram caracterizados como ambulatórios, atenção básica e sistema prisional, segundo autoclassificação dos respondentes ao inquérito Qualiaids (72,9%); para os não respondentes (27,1%) a classificação baseou-se nos termos presentes nos nomes dos serviços. RESULTADOS: No período, 238.599 pessoas com 15 anos ou mais iniciaram a terapia antirretroviral no Brasil, das quais, 69% receberam acompanhamento no SUS, 21,7% no sistema privado e 9,3% tiveram o sistema indefinido. Entre os acompanhados no SUS, 93,4% foram atendidos em serviços do tipo ambulatório, 5% em serviços de atenção básica e 1% no sistema prisional. CONCLUSÃO: No Brasil o tratamento antirretroviral é fornecido exclusivamente pelo SUS, que também é responsável pelo acompanhamento clínico-laboratorial da terapia da maior parte das pessoas em serviços ambulatoriais. O estudo só foi possível porque o SUS mantêm registros e informações públicas acerca do acompanhamento em HIV. Não há nenhum dado disponível para o sistema privado.
Impact of Diabetes on Outcomes in Patients Hospitalized With Acute Myocardial Infarction: Insights From the Atherosclerosis Risk in Communities Study Community Surveillance
Authors: Jain Vardhmaan , Qamar Arman , Matsushita Kunihiro , Vaduganathan Muthiah , Ashley Kellan E. , Khan Muhammad Shahzeb , Bhatt Deepak L. , Arora Sameer , Caughey Melissa C.
Published: May 2023

Background

Diabetes is associated with increased risk of acute myocardial infarction (AMI). The demographic trends, clinical presentation, management, and outcomes of patients with diabetes who are hospitalized with AMI have not been recently reported.

Methods and Results

The ARIC (Atherosclerosis Risk in Communities) study conducted hospital surveillance of AMI in 4 US communities. AMI was classified by physician review using a validated algorithm. Medications and procedures were abstracted from the medical record. From 2000 to 2014, 21 094 weighted hospitalizations for AMI were sampled. The prevalence of diabetes steadily increased, from 35% to 41% to 43% (
P
‐trend<0.0001) across 2000 to 2004, 2005 to 2009, and 2010 to 2014, respectively. Patients with diabetes were older (61 versus 59 years of age), more often Black (44% versus 31%), and more commonly women (42% versus 34%). The burden of cardiovascular comorbidities was higher with diabetes and increased temporally. Patients with diabetes less often presented with ST‐segment elevation (9% versus 17%) or acute chest pain (72% versus 80%), and had higher mean GRACE (Global Registry of Acute Coronary Syndrome) score (123 versus 109), Thrombolysis in Myocardial Ischemia (TIMI) score (4.3 versus 4.0), and Killip class (1.9 versus 1.5). Patients with diabetes had a lower adjusted probability of receiving aspirin (relative probability, 0.95 [95% CI, 0.91–0.99]), nonaspirin antiplatelets (0.93 [95% CI, 0.86–0.99]), coronary angiography (0.85 [95% CI, 0.78–0.92]), and coronary revascularization (0.85 [95% CI, 0.76–0.92]). Diabetes was associated with a 52% higher hazard of all‐cause 1‐year mortality (hazard ratio, 1.52 [95% CI, 1.23–1.89]).

Conclusions

Diabetes is associated with higher risk of death in patients hospitalized with AMI, highlighting the need for adherence to evidence‐based therapies in this high‐risk population.