bluuebunny/crossref_metadata_2025 huggingface dataset:
The identification and treatment of poor durability Karoo dolerite base course aggregate - evidence from case studies
Authors: Leyland R , Momayez M , van Rooy J
Published: April 2016

The Karoo Supergroup covers approximately 57% of South Africa's surface area and the sedimentary rocks therein generally do not yield acceptable pavement aggregates. The Karoo Dolerite Suite (intrusions) present in these sedimentary units have successfully been used as pavement aggregate sources, but numerous cases of premature pavement failure due to alleged rapid degradation of the dolerite have been reported. Durability tests are included in basic or mafic igneous rock aggregate specifications, but rapid pavement failures continue to occur. A study was recently undertaken to identify cases where degradation of Karoo dolerite was the cause of pavement failure. A secondary objective of the study was to determine if any observed degradation could have been identified using currently specified or alternative testing methods. Three such case study sites are presented in this article and the properties of their materials compared to those from five non-problematic dolerite materials. It is shown that the poor performance of the case study materials was likely due to the poor durability of the materials, manifesting as a reduction in resistance to abrasion and attrition. The identification of the observed poor durability could not have been performed accurately using only the currently specified test specifications. Alternative tests that allow an accurate differentiation to be made were, however, identified and, based on the results, tentative limits set. Additionally it was shown that modification of problematic Karoo dolerite base course materials, by applying lime at a rate less than the initial consumption of lime, can be successful in preventing further rapid pavement failures.
INFLUÊNCIA DO USO DE BISFOSFONATOS EM PACIENTES SUBMETIDOS A IMPLANTES DENTÁRIOS: REVISÃO DA LITERATURA
Authors: Santos Luciano Cincurá Silva , Pereira Renato Piai , Gusmão João Milton Rocha , Almeida Onily Duarte Silva de
Published: April 2016

O uso de implantes dentários como alternativa reabilitadora em pacientes com necessidade de restabelecimento do sistema estomatognático através da reposição das unidades dentárias é uma prática cada vez mais comum. Desafios sobre o manejo dos pacientes que apresentam algum fator adicional para a prática das reabilitações dentárias com implantes é uma constante, principalmente aquelas descritas na literatura acerca do insucesso envolvendo uso de fármacos à base de bisfosfonatos, sendo apontado diretamente como responsável pela presença de um quadro patológico denominado osteonecrose dos maxilares induzida por bisfosfonatos. Uma busca através de duas bases de dados foi realizada a fim de esclarecer dúvidas sobre a real influência dos fatores apresentados e sua verdadeira relevância na prática clínica, promovendo o conhecimento e estratégias de procedimentos mais coerentes nos casos que exijam a intervenção reabilitadora. Os resultados encontrados evidenciam que a necessidade da realização de novas pesquisas na área ainda é uma realidade.
IMPLANTE UNITÁRIO ANTERIOR PROCEDIMENTOS DE ENXERTIA E PROVISIONALIZAÇÃO: RELATO DE CASO
Authors: Matsumoto Wilson , Hotta Takami Hirono , Antunes Rossana Pereira de Almeida , Reino Danilo Maeda
Published: April 2016

A reposição de elementos dentais na região anterior envolve, principalmente, o aspecto estético. Este trabalho relata um caso de exodontia de incisivo lateral com instalação imediata de implante, enxertia de tecido mole, colocação de biomaterial e provisionalização, com o objetivo de manutenção estética. Os resultados clínicos imediatos, pós uma semana e com dois anos de uso da prótese definitiva mostraram-se satisfatórios do ponto de vista estético e funcional. Concluiu-se que é importante a realização de planejamento adequado para seleção e momento da execução de procedimentos clínicos necessários para o sucesso do tratamento.
AVALIAÇÃO DE CONHECIMENTO PRÉVIO SOBRE A DOENÇA FALCIFORME ENTRE PARTICIPANTES DE SEMINÁRIO DE ODONTOLOGIA
Authors: Castro Ilanna Jamile De Souza , Rêgo Emanuel Braga , Oliveira Diane Vasconcelos de Santana , Machado André Wilson , Trein Cristiano Augusto , Goldbeck Ana Stela
Published: April 2016

A Doença Falciforme (DF) é responsável por um elevado índice de mortalidade e morbidade no Brasil. É sabido que há diversas manifestações bucais e repercussões desta condição no tratamento odontológico. No entanto, pouco se conhece sobre o grau de conhecimento dos dentistas sobre esta enfermidade. O objetivo deste artigo foi avaliar o grau de conhecimento e o perfil de diferentes ouvintes, a partir de um questionário distribuído durante o seminário “O papel da Odontologia no contexto da atenção integral à DF”, realizado em um curso de Odontologia de uma instituição de ensino superior pública. Os resultados da presente pesquisa revelaram que, de uma forma geral, a maioria dos respondentes consideram ter pouco conhecimento sobre DF, apesar de possuírem uma grande disposição em aprender. Constatou-se também que há maior interesse neste tema por parte dos profissionais já formados, provavelmente em decorrência da demanda por atendimento de pacientes com DF no dia-a-dia da clínica, visto que a Bahia é o Estado brasileiro que apresenta a maior prevalência. Deste modo, evidencia-se que ainda há carência de informações e muitas dúvidas sobre o papel da Odontologia no contexto da atenção integral à DF. Uma maior atenção na formação do dentista no tocante às doenças de grande prevalência no Brasil pode promover a elaboração de uma agenda positiva, contemplando melhores condutas e protocolos terapêuticos adequados, traduzindo-se em um benefício real para o paciente.
Ectopic decidua of the greater omentum: a case report
Authors: Pigac Biserka , Masic Silvija
Published: April 2016

Ectopic decidua is defined as extrauterine deposits of decidual stromal cells. It occurs in 85-100% of pregnancies. Focal sites can be present in various locations, yet a peritoneal location is rare. A 24- year- old woman underwent a cesarean section in 39th week of her first pregnancy, during which adhesions of the omentum to the fundus, entire left side of the uterus, and a part of the right front abdominal wall were found. An operative specimen was taken for a pathohistological analysis under the assumption of being fibrous adhesive tissue. The analysis revealed ectopic decidual tissue composed of large, polygonal cells with eosinophilic cytoplasm, and large nuclei with conspicuous nucleoli infiltrated with mature fatty cells and lymphocytes. Strong staining for vimentin was observed in the decidual cell cytoplasm and for a progesterone- receptor in the cell nuclei, medium staining was detected for S-100, and negative staining for CK 5/ 6, HMB-45, desmin, smooth muscle actin, estrogen and androgen- receptors. We present this case in order to educate clinicians and pathologists about the phenomenon of ectopic deciduosis. Although it can exist as asymptomatic condition, we point out the importance of considering this condition since it can result in serious pathology, like intraperitoneal hemorrhage and labour obstruction, if remains unrecognized. Another pitfall is possible confusion of this entity with other conditions. A resemblance to adhesions of the omentum and malignant neoplastic lesions, like squamous cell and metastatic carcinoma, metastatic melanoma, malignant decidual mesothelioma, metastatic mucin -producing adenocarcinoma, can be deceiving. These obstacles may present a pitfall to clinicians and pathologists, with a negative impact on patient treatment and outcome.
Overexpression of genes encoding asparagine-glutamine rich transcriptional factors causes nonsense suppression in Saccharomyces cerevisiae
Authors: Nizhnikov Anton Aleksandrovich , Kondrashkina Aleksandra Mikhaylovna , Antonets Kirill Sergeyevich , Galkin Aleksey Petrovich
Published: April 2016

Previously, we have carried out a search for genes, whose overexpression causes nonsense suppression on the background of modified SUP35 variants expression in yeast Saccharomyces cerevisiae. Here we analyzed the influence of genes encoding N/Q-rich transcriptional factors on this process. We demonstrated that GLN3, MCM1, MOT3 and REB1 genes overexpression affects nonsense suppression in S. cerevisiae
Rol-GENES of Agrobacterium rhizogenes
Authors: Pavlova Olga Andreyevna , Matveyeva Tatyana Valeryevna , Lutova Lyudmila Alekseyevna
Published: April 2016

The review summarizes the information about rol-genes, which constitute a part of the T-DNA of Agrobacterium rhizogenes Ri-plasmid. Structure of each of the rol-genes, their regulatory sequences, and the possible roles of rol-genes when they are being transferred into the plant are discussed
Research of the influence of LIG4 gene polimorphism on chromosomal aberrations level in human lymphocytes, with background and excessive exposure to radon
Authors: Volkov Aleksey Nikolayevich , Golovina Tatyana Aleksandrovna , Druzhinin Vladimir Gennadyevich , Larionov Aleksey Viktorovich , Minina Varvara Ivanovna , Timofeyeva Anna Aleksandrovna
Published: April 2016

Effects of the LIG4 gene polymorphisms rs1805388 (Thr9Ile) and rs1805389 (Ala3Val) on the level of chromosomal aberrations in peripheral blood lymphocytes have been estimated in children, that live and study in conditions of normal and excessive exposure to radon in several localitions of the Kemerovo region. There was no statistically significant difference between genotypic groups on the marker rs1805388 with regard to frequencies of cytogenetic indexes. At the same time, in the group of Val carriers (marker rs1805389) from second cohort our analysis revealed significant increase in the amount of paired fragments in comparison with homozygotes Ala/Ala.
Genetic predisposition to helminthiasis
Authors: Saltykova Irina Vladimirovna , Freydin Maksim Borisovich , Ogorodova Lyudmila Mikhaylovna , Puzyrev Valeriy Pavlovich
Published: April 2016

Helminthes accompany human beings from the early period of the formation, there is a long-term coevolution between parasite and human, helminthes represent a major selective force for human immune genes. Data on genetic control of the intensity and clinical traits of helminthinfection in different populations, including results obtained by whole-genome studies are presented and concept of common genes of susceptibility to helminth infection and allergic diseases are discussed.
Investigations of possible horizontal gene transfer from Agrobacterium to some representatives of the family Solanaceae
Authors: Kulayeva Olga Alekseyevna , Matveeva Tatiana Valeryevna , Lutova Lyudmila Alekseyevna
Published: April 2016

The development of methods of genetic engineering of plants poses in modern society the question of the safety of transgenic plants. Transformation of plants has been criticized as artificial process performed only in the laboratory. At the same time there is evidence that some members of the genus Nicotiana and Linaria contain sequences homologous to the T-DNA of Agrobacterium rhizogenes in their genomes. The question: whether there are other examples of horizontal gene transfer from Agrobacterium to the plant among the representatives of the family. Solanaceae, is still actual. We performed screening of representatives of four genera of the family Solanaceae for the presence of sequences homologous to oncogeniclike genes rolB, rolC, ORF13, ORF14. New examples of horizontal gene transfer were not detected. Apparently, the presence of sequences homologous to oncogenes of A. rhizogenes in the genome of nontransformed plants is not common to the whole family Solanaceae and is characteristic only of the some species of the genus Nicotiana.
Research of genetic markers in susceptibility to arterial hypertension in Russian Northwest region children
Authors: Kanayeva Mariya Dmitriyevna , Glotov Andrey Sergeyevich , Vashukova Yelena Sergeyevna , Obraztsova Galina Igorevna , Baranov Vladislav Sergeyevich
Published: April 2016

Вy PCR-RFLP procedures in groups of children of the Northwest region of Russia we investigated associations of some variants of CYP2J2, CYP4A11 and PTGIS genes participating in a metabolism of arachidonic acid and variants of CDH13, MTHFR, CDKN2BAS genes, and also an extra gene molecular marker rs11191548 T/C revealed on the basis of the GWAS method as candidates of risk of arterial hypertension, with development of a hypertension. The association of these markers with development of a hypertension in children didn't prove to be true.
Association of vitamin D receptor gene with anthropometric measures in komi ethnic group
Authors: Kozlov Andrey Igorevich , Vershubskaya Galina Grigoryevna , Ateyeva Yuliya Aleksandrovna , Orr Pamela , Larcombe Linda
Published: April 2016

The relationship between vitamin D receptor gene (VDR) variants with serum 25-OHD3 concentration, body height (BH), body weight (BW), and body composition were examined in Komi ethnic group. The FF genotype associates with higher BW (p=0.002), and lower bone mass (BM, p=0.06) in comparison with the Ff subjects. The BB carriers are shorter than those with Bb genotype (p=0.037); BM is lower among having bb than Bb variants (p=0.025). There were no differences in 25-OHD3 content revealed. The results are consistent with the data obtained in populations from North-Western Europe, but not in tropical and subtropical Caucasians, or in non-Caucasian groups.
Biosynthesis of hexaand pentameric chitooligosaccharides using n-acetylglucoseaminyl transferase from rhizobial bacteria
Authors: Leppyanen Irina Viktorovna , Artamonova Tatyana Olegovna , Lopatin Sergey Aleksandrovich , Varlamov Valeriy Petrovich , Tikhonovich Igor Anatolyevich , Dolgikh Yelena Anatolyevna
Published: April 2016

Chitooligosaccharides find wide application that determines considerable interest in their use. Enzymatic synthesis of hexa-N-acetylchitohexaose and penta-N-acetylchitopentaose using N-acetyl-glucoseaminyl transferase enzyme possessing unique features from rhizobial bacteria Rhizobium sp. GRH2 and M. loti has been performed in E. coli cells. Cultivation of bacteria E. coli expressing the appropriate recombinant enzyme resulted in synthesis of significant amounts of desired chitooligosaccharides (milligrams per liter). Analysis of synthesized chitooligosacchairdes by methods of high performance liquid chromatography and mass-spectrometry confirmed the conformity of the synthesized compounds to standards.
Genetic control of chlorophyll metabolism
Authors: Chekunova Elena M
Published: April 2016

Chlorophylls (Chl) are unique tetrapyrrole molecules, essential not only for photosynthesizing organisms but for the whole Biosphere. Chlorophyll biosynthesis is closely connected with plant cell morphogenesis and photosynthetic reactions - storage and transferring of light energy. Pigment mutants of plant and microorganisms are successfully used for investigation of Chl biosynthesis and degradation pathways. The genetic approaches appeared to be very productive for identification of the genes, encoding the enzymes of Chl metabolism and for elucidation of the mechanisms there regulating. History, recent findings and evolution of genetic determination of Chl formation processes are presented in this review.
Features of the organization and functioning of a unique class of plant receptor kinases containing lysm-motives in the extracellular domain
Authors: Kirienko Anna Nikolaevna , Leppyanen Irina Viktorovna , Dolgikh Elena Anatolyevna
Published: April 2016

Analysis of current data concerning functioning, structural organization and evolutionary aspects of origin for a unique class of the plant LysM-receptors has been performed. Plant receptors with LysM-motifs in the extracellular domain act as mediators in recognition of N-acetylglucosamine-containing compounds. Such compounds from pathogenic bacteria and fungi cause activation of plant defense systems, while the compounds secreted by symbiotic microorganisms trigger endosymbiosis formation. A possible mode of receptor operation in binding of structurally similar microbial signals, that leads to pathogen resistance or endosymbiosis development, as well as the role of LysM-receptors in these processes, have been examined.
Identification of filamentous green algae from an area of local biogenic pollution of Lake Baikal (Listvennichnyy bay) using SSU 18S rDNA molecular marker
Authors: Romanova Elena Vladimirovna , Kravtsova Lyubov Sergeevna , Izhboldina Lyudmila Aleksandrovna , Khanaev Igor Veniaminovich , Sherbakov Dmitriy Yuryevich
Published: April 2016

Background. A local eutrophication process is noted for the first time in Listvennichnyi bay in Lake Baikal. High level of biogenic elements in the water of the bay led to nuisance growth of filamentous algae. Rapid identification of these algae's taxa was necessary to assess ecosystem damage in the polluted area of the lake. Materials and methods. We determined a boundary of bottom fouling by locating transects ranging from 0 to 10 meters in different areas of the bay. Algae samples were examined using microscopic techniques as well as SSU 18S rDNA sequencing. Phylogenetic trees were estimated using Bayesian inference (BI) based on SSU alignment. Results. Our results showed intensive filamentous algae fouling in depth from 3 to 10 meters along the coastline of the eastern part of the bay. Morphological and molecular analysis demonstrated prevalence in samples of Ulothrix zonata (Web. et Mohr) Kütz. Using SSU 18S rDNA sequencing data we also showed presence of three species of Spirogyra Link. genus. Conclusion. Detection of Spirogyra species which are normally not typical for such areas of the lake along with Ulothrix zonata extended more deeply than usually observed reveals severe phytocenosis disturbance of the polluted part of Lake Baikal.
Genetic and morphological diversification in gastropods of the Baicaliidae family
Authors: Kovalenkova Mariya Vladimirovna , Sitnikova Tatyana Yakovlevna , Shcherbakov Dmitriy Yuryevich
Published: April 2016

Background: Molecular phylogenetic studies of Baikalian endemic gastropod family Baicaliidae as well as the morphological comparisons have not yet provided a fully resolved phylogeny of this family. There is a need to increase the current set of markers to solve current difficulties. Intronic nuclear markers can be used as a valuable tool for phylogenetics. Methods: Nucleotide sequences for the intron of ATP-synthase alpha-subunit gene from 11 gastropod species belonging to the fast evolving Baikalian endemic family Baicaliidae together with the morphological traits and previously obtained mitochondrial COI sequences were used to build a synthetic species tree. Results: A Phylogenetic tree built using only intron sequences contains less polytomies than the one built using sequences of the mitochondrial gene CO1 and is compatible with the morphological views. Intronic marker provides high support for the interspecific clades. Topology of the tree built using the intronic marker mainly corresponds to morphology based systematics of eleven investigated species of this family. The only exception was Godlewskia wrzesniowski, which were placed within the clade of species from Korotnewia and Parabaikalia genera, though it has significant morphological differences from these genera. Conclusions: Sister species were shown to diverge within the same substrate preferences. Observed discrepancies between the species tree and current taxonomy of the group may be explained by fast morphological evolution in the Baicaliidae family.
Divergence in expression of PHO3, 5, 11, 12 paralogues yeasts is the mechanism guiding the evolution of multigene families
Authors: Sambuk Elena Viktorovna , Padkina Marina Vladimirovna
Published: April 2016

This review considers evolution of multigene families based on the example of the PHO gene family, which encodes acid phosphatases. An analysis of databases revealed that the divergence of regulation of structural genes transcription and their involvement in novel regulatory pathways, is the main direction in evolution of multigene families.
POLYMORPHISM OF EXCISION REPAIR GENES XPD, XRCC1, hOGG1 IN THE POPULATION OF THE REPUBLIC OF BELARUS AND ITS IMPACT ON CARCINOGENESIS
Authors: Ramaniuk Volha Petrovna , Nikitchenko Natalya Vasilyevna , Savina Natalya Viktorovna , Kuzhir Tatyana Danovna , Goncharova Roza Iosifovna
Published: April 2016

Background. DNA damage and induced mutational events are known to contribute notably to carcinogenesis, so the study of excision repair gene polymorphisms and their association with cancer risk is of great interest and importance. Materials and Methods. Excision repair gene polymorphisms (XRCC1 Arg399Gln, hOGG1 Ser326Cys, XPD Asp312Asn) were analyzed using a PCR-RFLP method in the group of bladder cancer (BC) patients compared to clinically healthy individuals. Results. In the healthy population, the frequencies of the minor alleles of XPD 312, XRCC1 399, hOGG1 326 genes were 42,1 %, 35,4 % and 24,4 %, respectively, i. e., in the range of values observed in Caucasian populations. The frequencies of genotypes/alleles in the group of BC patients did not differ from those in the control group. However, the frequency of Asn allele of XPD gene was significantly higher in men with BC recurrences as compared to primary tumors. The cancer risk was decreased in carriers of combined Asp/Asp, Arg/Arg, Ser/Ser genotypes of XPD, XRCC1 and hOGG1 genes (OR95 %CI = 0,46 [0,23 - 0,91] p = 0,024 and OR95 %CI = 0,42 [0,18 - 0,98] p = 0,045 for combination of two and three genes, respectively). Conclusion. In Belarus, the frequencies of the XPD, XRCC1 and hOGG1 minor alleles are similar to those in Caucasian populations. Although single nucleotide polymorphisms investigated did not affect the risk of bladder cancer, the risk of cancer recurrence was increased in carriers of the XPD Asn allele. Combined homozygous wild type alleles of indicated excision repair genes appeared to possess a protective effect against carcinogenesis.
Simulation of evolution of the legume-rhizobia symbiosis under the conditions of ecological instability
Authors: Vorobyov Nikolay Ivanovich , Provorov Nikolay Alexandrovich
Published: April 2016

The mathematical model is constructed which describes the impacts of chaotically changing environment on the frequencies and productivity of partners in the legume-rhizobia symbiosis. The most sensitive for external impacts are the adaptively prospective bacteria strains which are specific with respect to hosts and are capable for the intensive evolution towards an improved symbiotic efficiency. An increased stability of these strains in symbiotic system may be an important factor of its evolution for the improved efficiency of partners’ interaction.
Cytokine gene polymorphisms and early pregnancy loss
Authors: Mashkina Yelena Vladimirovna , Kovalenko Konstantin Alekseyevich , Fomina Nadezhda Vladimirovna , Shkurat Tatyana Pavlovna
Published: April 2016

Background: A disbalance of pro- and anti-inflammatory cytokines can negatively affect early stages of human embryogenesis. The association between polymorphism of cytokine genes (-31С-Т IL1B, -174G-C IL6, -308G-A TNFα and -592С-A, -819C-T IL10) and a pregnancy loss was studied. Materials and method: Study was performed on DNA samples from two groups of women with pregnancy loss: those with a missed abortion (MA) (n = 62) and those with a spontaneous abortion (SA) (n = 62). The control group included 114 women with normal pregnancy. Cytokine genotyping was performed using PCR with sequence-specific primers, with the “SNP-express” kit (Lytech, Russian Federation). Results: Increase in the frequency of heterozygotes for -31С-Т polymorphism of the IL1β gene among women with MA (58.1) if compared with those with SA (36.7%) was found. In the SA group the frequency of heterozygotes for -592С-Т of the IL10 gene was higher (56.7%) than in the control group) (32.5%). The frequency of the -819Т allele of IL10 gene among women with SA was higher than in the control group (0.33 vs 0.23). The -308A allele of the TNFα gene featured the lowest frequency in the SA group if compared with those in the MA and the control group). Conclusion: Our data show that the risk of spontaneous abortion increases if -592A and -819T IL-10, and -308G TNFα genes` alleles are present in the genotype.
Morphogenetic analysis of populations of Helix pomatia L. (Pulmonata, Helicidae) on the south-eastern and eastern part of the current area
Authors: Snegin Eduard Anatol'yevic , Artemchuk Olesya Yur'yevna
Published: April 2016

Background. This article is about evolutionary processes in populations of small mobile species in the urbanized landscape. The evolutionary processes of a small mobile species in the urbanized landscape are considered in this article. Materials and methods. The morphological and genetic variety of the Helix pomatia L. populations was studied by the morphometric method and the polyacrylamyd gel electrophoresis method. Samples were collected from twelve landscape urbanized populations from south-eastern and eastern part of the current Helix pomatia L. area. Results. According to the data, the majority of the study populations of this mollusk have the satisfactory condition. The most populations are characterized as high level of heterozygosity, the low inbreeding and the large effective size values of the population testify to this. The structure of the gene pools in the populations snails in the study area is determined by the origin of these populations, the genetic-automatic processes and the microclimatic conditions of the urban environment.
Influence of phytoecdysteroids and plants steroidal glycosides on the lifespan and stress resistance of drosophila melanogaster
Authors: Shaposhnikov Mikhail Vyacheslavovich , Shilova Lyubov' Alekseevna , Plyusnina Ekaterina Nikolaevna , Volodina Svetlana Olegovna , Volodin Vladimir Vital'yevich , Moskalev Aleksey Aleksandrovich
Published: April 2016

Background. Elucidation of the molecular mechanisms of effects of the active substances of plant adaptogens is a topical area of researches. Materials and methods. We studied the effect of herbal substances containing phytoecdysteroids (20-hydroxyecdysone and inokosterone) of Serratula coronata L. or steroidal glycosides (dioscin and protodioscine) of Trigonella foenum-graecum L. on the expression level of stress response genes (genes of heat shock proteins, DNA repair, antioxidant defense and apoptosis), stressresistanse (paraquat, starvation, hyperthermia) and lifespan of Drosophila melanogaster. Results. The studied herbal substances upregulated genes of antioxidant defense mechanisms (Sod1), but downregulated the DNA repair (XPF and Rad51) and apoptosis (Hid) genes. At the same time herbal substances induced weak adaptogenic and antiaging effects. Conclusion. Our results demonstrate that the herbal substances containing phytoecdysteroids and steroidal glycosides change the expression level of stress-response genes and activate mechanisms of hormesis.
Creation of new pichia pastoris strains for recombinant protein production
Authors: Muzaev Dmitriy Mikhaylovich , Rumyantsev Andrey Mikhaylovich , Sambuk Elena Viktorovna , Padkina Marina Vladimirovna
Published: April 2016

Pichia pastoris yeasts are widely used as a production platform for heterologous proteins. Wide biotechnological use of these yeasts is determined by simplicity of cultivation, cheap media and ability to provide posttranslational modifications. Basic approaches for enhancement of the recombinant protein outcome constitue increasing number of gene copies, which encode target protein, as well as co-expression of supporting factors for protein folding, processing and secretion. Development of relevant plasmids and auxotrophic strains is essential for solving these tasks. In this study, we report plasmids and strains collection, whichwill allow to conduct integration of multiple foreign genes in P. pastoris genome
Statistical approach to the problem of the taxonomic status and evolutionary potential of a non-biting midge Prodiamesa olivacea Meigen (1818) (Diptera, Chironomidae, Prodiamesinae) based on karyotype analysis
Authors: Mylnikov Sergey Vladimirovich , Zhirov Sergey Vital'evich
Published: April 2016

The frequency of contacts of centromere and telomere regions of polytene chromosomes in the karyotype of a midge Prodiamesa olivacea has been analyzed. The fact of non-random frequency of centromere regions contacts has been confirmed by statistical methods. These data are consistent with the diploid number of chromosomes equal to 10. The species has been announced as a “transitional form”, evolving towards reduction of the chromosome number.
First data on genetic variability of the siberian wood frog rana amurensis in western siberia and its differentiation from the moor frog rana arvalis
Authors: Zhigileva Oksana Nikolaevna , Kirina Irina Yur’yevna
Published: April 2016

The article presents data on genetic variability in populations of two brown frog species: the moor frog Rana arvalis and the siberian wood frog R. amurensis, in Western Siberia, Russia. Persentage of polymorphic ISSR-PCR-bands in R. arvalis was 63-93 %, in R. amurensis - 90 %, genetic diversity indices were 0.18-0.20 and 0.31, respectively. The high level of genetic variability in the siberian wood frog is contrary to its low population size, restricted distribution in the study area and the boundary position of the population. Some ISSR-PCR-bands were species-specific, they can be used for fast genotyping and further population genetic studies of the siberian wood and the moor frog in their areas of cohabitation.
Mitochondrial DNA d-loop polymorphism analysis for estimation of diversity in chicken flocks of Pavlov breed
Authors: Demin Alexander Gennadievich , Danilova Maria Igorevna , Galkina Svetlana Anatolievna
Published: April 2016

Elucidation of the complex origin of various chicken breeds and populations is of essential importance for understanding, preserving and exploiting their genetic diversity. Here, we aim to assess different contributions to mitochondrial genetic diversity of Pavlov chicken breed. Mitochondrial DNA control region (D-loop of 1231/1232 b. p. length) in 37 chickens of Pavlov breed was sequenced. Individuals were selected from three flocks belonging to Federal State Unitary research farm “Gene Pool” (Genofond), Pushkin, Leningrad region, to the collection farm of All-Russian R D and Technology Institute of Poultry Industry (GNU VNITIP), Sergiev Posad, Moscow region, and to fancy breeders from Barnaul (Altai region). The Pavlov chicken D-loop sequences were compared with D-loop sequences annotated in GenBank for established chicken haplogroups. We have found eleven haplotypes belonging to two haplogroups (E1 and A). Genetic uniformity and stability have been shown for the GNU VNITIP and Barnaul flocks, while D-loop high polymorphism was found in the population from the research farm “Gene Pool”. There appears a tendency for genetic fragmentation of Pavlov chicken breed.
High hydrostatic pressure influence on viability and mutagenesis of Salmonella Typhimurium
Authors: Karamova Nazira Sunagatovna , Zelenikhin Pavel Valer’evich , Kiselev Vladimir Dmitrievich , Lipatnikova Anastasiya Alexandrovna , Ilinskaya Olga Nikolaevna
Published: April 2016

Background: pressure is a well-known physical environmental parameter. Nevertheless, the basic principles of microbial survival under high hydrostatic pressure (HHP), especially genetic response to pressure, are still poorly understood. The purpose of this study was to investigate the influence of HHP ranging from 50 to 800 MPa on viability and mutagenesis of Salmonella typhimurium. Materials and methods. The standard plate count method (counting the total number of colony forming units (CFUs) on the plate) and the propidium iodide (PI) flow cytometric assay were used to determine the bacterial viability after HHP treatment. Ability of HHP to induce gene mutations was examined by the Ames assay employing Salmonella typhimurium TA100 and TA98. Results. The results obtained showed that survival of S. typhimurium cells considerably decreased when bacteria were exposed to a pressure of 200 MPa and above. Herewith, the survival index calculated according to the total number of CFUs was up to six orders of magnitude lower than that obtained by the flow cytometric analysis under the same HHP. This fact can be explained by the entrance of the some part of bacterial population into the viable but nonculturable (VBNC) state. The pressure of 50 MPa was found to cause a 1.9-fold increase in the number of His+ revertants of S. typhimurium TA98 in Ames test. Conclusion. Our results demonstrate that HPP of 200 MPa and above significantly inhibits the viability of S. typhimurium cells as well as triggers the induction of VBNC state. The results of Ames test suggest that HHP of 50 MPa can induce gene mutations in bacterial cells. The possible mechanisms of HHP effects on cells viability as well as genetic response of bacteria under HHP are discussed.
Neural Basis for the Relationship Between Academic Motivation and Academic Achievement
Authors: Chakraborty Rajib
Published: April 2016

The present study is an attempt to provide evidence to the neural basis of the relationship between academic motivation and academic achievement constructs. Sample for the study includes 49 students (25 girls and 24 boys) from VIIIth and IXth classes of a secondary school in Sriram Nagar, Hyderabad, Telangana, India. The data for measuring academic motivation is collected using Academic Motivation Scale, High School Version (AMS-HS 28) for high school students prepared by Vallerand and et.al (1992). Academic achievement of the students is measured by collecting the students’ grade point average in a summative assessment. For data analysis, Pearson’s Product-Moment Correlation coefficient is used. The significance of the test is calculated using critical value table for Pearson’s Product-Moment Correlation for the level of significance α at 0.05. The findings of the study establish the prefrontal cortex of the brain as the neural basis for the relationship between academic motivation and academic achievement in the chosen subjects.
Dimensional Analysis of the Academic Motivation Scale with Respect to Gender in Secondary School Students with Neural Basis in Indian Context
Authors: Chakraborty Rajib
Published: April 2016

The present study is an attempt to provide a neural underpinning to the role of gender difference in the construct academic motivation, in secondary school students in Indian context. For this purpose, comparisons of students’ scores of the dimensions of academic motivation were carried out. Samples for the study include 49 urban students (24 boys and 25 girls ) of classes VIII and IX of secondary school in Sriram Nager of Hyderabad city, Telangana, India. Academic motivation construct is measured by using Academic Motivation Scale, High School Version (AMS-HS 28) for high school students prepared by Vallerand and et.al (1992). For data analysis, measures like mean, standard deviation are used. The significance of the test is calculated using t-test for the level of significance α at 0.05. The findings of the study reveal no differences between the genders in the measured dimensions of academic motivation.
Grazing and Work Stress on The Live Weight Change of Mashona Oxen
Authors: Prasad V. L. , Mandebvu P. , Mhonde C.
Published: April 2016

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Effects of Traditional Medicine on Fat-Tailed Sheep's Performance
Authors: (Soebarinoto) Soebarinoto , Astuti T. Y. , (Hermanto) Hermanto , (Marjuki) Marjuki
Published: April 2016

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Pre and Post-Weaning Performance Characteristics of Brahman, Boran and Tuli Crosses in Beef Cattle Evaluation Programs
Authors: Rowan K. J. , Taylor D. G. , Josey M. J.
Published: April 2016

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The Effect of Corpora Luteal Number on Serum Progesterone and Estradiol of Ewes During Luteal Phase of Estrous Cycle and Pregnancy
Authors: Sumaryadi M. Y. , Manalu W.
Published: April 2016

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Effects of Dietary Levels of Energy and Protein at the Finishing Stage. 1. Performance and Pelt Production of Rex Rabbits
Authors: Habibie A. , Kusnidar D. , Sartika T. , Raharjo Y. C.
Published: April 2016

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Profitability of Intensifying Kampong Chicken in Relation to Village Poverty Alleviation
Authors: Jarmani S. N. , Nataamidjaja A. G.
Published: April 2016

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Effect of Using Salt and Sodium Tripolyphosphate (STPP) on Meat Ball Quality
Authors: Sunarlim R.
Published: April 2016

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Analysis of Developed Yoghurt using Milk form Goat and Cow Reared at Local Feed in Sleman
Authors: Murti Tridjoko Wisnu
Published: April 2016

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Effect of Rams, Incubation Time and Dilution Rate on The Motility and Longevity Characteristics of Merino Rams Spermatozoa
Authors: (Ismaya) Ismaya
Published: April 2016

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Ration Evaluation for In Vitro Digestibility Application Using Cattle and Buffalo Faeces as Inoculum
Authors: (Sudirman) Sudirman , Utomo R. , Bachruddin Z. , Widyobroto B. P. , (Dahlanuddin) Dahlanuddin
Published: April 2016

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Karakteristik Sosis dengan Fortifikasi β-Caroten dari Labu Kuning (Cucurbita moschata) (Sausage Characteristics as Affected by β-Caroten Fortification of Pumpkin (Cucurbita moschata))
Authors: Prayitno Agus Hadi , Miskiyah Firdha , Rachmawati Afina Viyunnur , Baghaskoro Tombak Mahesa , Gunawan Bekti Putra , (Soeparno) Soeparno
Published: April 2016

The objectives of the experiment were to evaluate physical, chemical, and sensory characteristics of sausage by using fortification with β-caroten of pumpkin. There were five treatments of fortification with β-caroten of pumpkin as
the filler substitutions on sausage processing. The treatments were: 0% (control), 25, 50, 75, and 100% of the filler. There were five replications in each treatment. The data of physical and chemical characteristics from the completely randomized design were analysed by analysis of variance. The data of sensory characteristics were analysed by the analysis of non parametric test of Hedonic Kruskal-Wallis. The results showed that sausage fortified with β-caroten of pumpkin as the filler substitutions up to 100% level on sausage processing affected significantly (P<0.01) on physical characteristics of sausage (decreased water-holding capacity, increased tenderness and it did not affect pH value of sausage), chemical characteristics of sausage (decreased moisture, increased protein, fiber, β-caroten, and it did not affect the fat content of sausage), and sensory characteristics of sausage (increased taste, decreased color, aroma, texture, touchness, and acceptability of sausage).

(Key words: Sausage, Pumpkin, β-caroten, Physical characteristics, Chemical, Sensory)
Kinerja Reproduksi Ternak Kuda Kerja di Kabupaten Bantul, Daerah Istimewa Yogyakarta (Reproductive Performances of Working Mare at Bantul Regency, Yogyakarta Special Province)
Authors: Setyobudi Arif , (Kustono) Kustono , Widayati Diah Tri
Published: April 2016

The experiment was conducted to determine reproductive performances of working mare at Bantul Regency, Yogyakarta Special Province. One hundred and fourten mares were used in the experiment coming from 94 respondens.
Samples were collected from district of Sewon, Banguntapan and Pleret. The parameters observed were first mating age, service per conception (S/C), postpartum mating (PPM), foaling interval (F/I), body weight, working hours, and
feed consumption. The collected data were analyzed descriptively. The average of first mating age, service per conception (S/C), postpartum mating (PPM), foaling interval (FI) were 31.42±4.62 months, 2.85±0,81; 94.69±32.42
days; 493.65 ± 43.01 days (ranged in 1-5 S/C; 35-180 days; 395-594 days); body weight, working hours, and feed consumption were 273.65±43.64 kg, 8 hours 11 minutes/day, 16.02±2.17 kg respectively. The conclusion of the
experiment were that the reproductive performances of working horse at Bantul Regency in Daerah Istimewa Yogyakarta is generally less than the normal performances reproductive of horse.

(Key words: Reproduction performance, Working horse, Bantul Regency)
PERUBAHAN FENOTIP SAPI PERANAKAN ONGOLE, SIMPO, DAN LIMPO PADA KETURUNAN PERTAMA DAN KETURUNAN KEDUA (BACKCROSS)
Authors: (Trifena) Trifena , Budisatria I Gede Suparta , Hartatik Tety
Published: April 2016

The aims of the study was to analyze the phenotype of Ongole Grade, SIMPO, and LIMPO cows based on the exterior characteristics and body size of cattle, and to distinguish the phenotype of the first filial (F1) and the backcross
(BC1) of SIMPO and LIMPO cows, reared by farmers at Pacitan, East Java. The study was conducted with purposive sampling method, involving 5 districts: Pacitan, Arjosari, Pringkuku, Nawangan, and Punung. Eighty four farmers as
respondents with total of 100 head cattle were used in the study. The results showed that the dominant coat color in Ongole Grade was white. However, none of crossbreed in F1 and BC1 had white color, but the color phenotype change
to brown and dark brown, which increase the dark intensity. The muzzle color of Ongole Grade was black, and changed to red in BC1. The hoof color’s of Ongole Grade was black, and brown for BC1. The color phenotype of F1 was similar
to both color of Ongole Grade and BC1 phenotype. The increasing intensity of coat color in F1 to BC1 indicated that there was a combination of gene from Simmental or Limousin with F1 and BC1which containing of two or several set of
genes. SIMPO and LIMPO cows had greater body size than those of Ongole Grade cows. The body’s sizes of BC1 was larger than F1 in SIMPO and LIMPO cows, which was influenced by increasing the effect of additive genes from F1 to
BC1.

(Keywords: Ongole Grade, SIMPO, LIMPO, First filial, Backcross, Phenotype of cattle)
Campos e Redes na Análise das Organizações: Explorando Distinções Teóricas e Complementaridades Metodológicas
Authors: Alvarez Candido Silvio Eduardo , Sacomano Neto Mário , Côrtes Mauro Rocha
Published: April 2016

Neste ensaio, exploramos as distinções teóricas e complementaridades metodológicas existentes entre abordagens que conceitualizam as estruturas sociais com base nas noções de campos e redes. Essas perspectivas se opõem tanto à visão atomizada quanto à supersocializada da ação e buscam superar a dicotomia entre estrutura e agência, mas possuem diferenças conceituais importantes Aspectos conceituais são discutidos a fim de explicitar as diferenças entre as abordagens enfocadas. Apontamos que a forma como a noção de campo é utilizada por autores como Pierre Bourdieu e Neil Fligstein é articulada em um referencial teórico relacional, no qual a definição da estrutura é inseparável da de agência e do poder, possibilitando a superação de limitações das estruturas como redes. Enfim, argumentamos que o uso das técnicas de análise de redes pode ser orientado pelas teorias de campo.
The anthropology of film in Serbia
Authors: Kovačević Ivan , Ilić Vladimira
Published: April 2016

The term ‘film’ refers to feature length commercial movies both of foreign and domestic production. The phenomenon of film is approached as a form of popular culture which means that the emphasis is on its cultural-communicative traits. The paper aims to make visible the dynamic field of anthropology of film present in Serbian academia, as well as introduce readers to this subdiscipline and point them towards the relevant literature – both foreign and domestic – in the field. The paper has been envisioned as an introduction to the edited volume Anthropology of film – the first book in the new series of editions titled New Serbian anthropology. Six anthropological and two sociological papers which together make up the edited volume are presented here. The aim is to highlight the thematic breadth and wealth of research in this area of anthropology.
The Ideas of 1914 – German Intellectuals and the Legitimation of the German Reich's Policy at the Beginning of the First World War
Authors: Antolović Mihael
Published: April 2016

After the outbreak of the World War in august 1914, German intellectuals – university professors, writers, artists and publicists – gave an important contribution to the legitimation of the German Reich’s war aims. As a kind of ‘spiritual mobilization’ for the sake of the defense of the fatherland from the allied enemies, they engaged in intense publicity and propaganda activities whose goal was to show the responsibility of the opponent countries for the outbreak of the war as well as to prove that Germany was fighting a just war for its national survival. The  ideological justification of the German war aims formulated in the so called ‘ideas of 1914’ rested on several motives. First, the  French revolution and its entire political, social and conceptual inheritance were discarded. The revolutionary transformation of society, the  theory of natural law, the liberal and democratic ideology and the  parliamentary system consider to be inappropriate to ‘German national spirit’, German intellectuals argued for  the superiority of gradual social reforms, conservative social and political order and authoritarian political system embodied in the Hohenzollern monarchy. At the same time, they rejected the modern class society marked by the predominance of the  egoistic individual interests in favour of an allegedly harmonious ‘organic community’. Finally, they attributed some higher metaphysical meaning to the current war since it was conceived of as a battle for the preservation of the German culture understood as sublimation of highest spiritual achievements of the entire humanity, from the decadent materialistic civilization of Western Europe as well as ‘barbarian’ Russia. In that way, in the ‘ideas of 1914, German intellectuals expressed all elements about the peculiarities of German history (der deutsche Sonderweg) thus giving ideological legitimacy  to the German Reich’s war endeavours and to its ‘grab for world power’.
Representation of war heroes in TV series “The Sacrificed” (“Otpisani”)
Authors: Simeunović-Bajić Nataša
Published: April 2016

Domestic research in the fields of culture, communications and media has not mostly dealt with the ideological and political aspects of popular TV programs and their role in forming dominant cultural patterns in the second half of the twentieth century. Therefore, this paper represents an attempt to refocus the attention of researchers to profounder readings of ideological and discursive meanings of the popular television culture of the Yugoslav era, and the TV series The Sacrificed (Otpisani) has been chosen for such a new and different view and analysis. Although made in the widely established discourse of the Yugoslav community and socialism, with the substantial financial funds and creative force of the creators of the project, it also announces discursive rifts of different orders and great socio-historical cuts. Also, it establishes new cultural patterns in the building of cultural identity and collective memory of several generations raised on the former Yugoslav territory.