bluuebunny/crossref_metadata_2025 huggingface dataset:
Ion Irradiation for Planar Patterning of Magnetic Materials
Authors: Kato Takeshi , Oshima Daiki , Iwata Satoshi
Published: January 2019

Kr+ ion dose dependence of the magnetic properties of MnGa films and the fabrication of planar-patterned MnGa films by the local ion irradiation technique were reviewed. The magnetization and perpendicular anisotropy of the MnGa vanished at an ion dose of 1 × 1014 ions/cm2 due to the phase change of the MnGa from ferromagnetic L10 to paramagnetic A1 phase. The average switching field Hsw of the planar-patterned MnGa increased with decreasing the bit size, implying low bit edge damage in the patterned MnGa, whereas a rather large switching field distribution (SFD) of 25% was confirmed for a bit size of ~40 nm. Time resolved magneto-optical Kerr effect measurements revealed that as-prepared MnGa exhibits an effective anisotropy field Hkeff = 20 kOe, its distribution ΔHkeff = 200 Oe, and Gilbert damping α = 0.008. The ion-irradiated MnGa films exhibited larger Hkeff = 22–23 kOe than that of the MnGa before the ion dose. Thus, ion irradiation does not decrease the perpendicular anisotropy, which suggests a small bit edge in the patterned MnGa. ΔHkeff increased from 0.2 kOe to 3 kOe, whereas the length of disorder in the film ξ decreased from 10 nm to 3 nm by ion irradiation.
Effect of Tip Clearance on Flow Field and Heat Transfer Characteristics in a Large Meridional Expansion Turbine
Authors: Meng Fusheng , Zheng Qun , Gao Jie , Fu Weiliang
Published: January 2019

The large meridional expansion turbine stator leads to complex secondary flows and heat transfer characteristics in the blade endwall region, while the upstream tip clearance leakage flow of the rotor makes it more complex in flow and heat transfer. The influence of the upstream rotor tip clearance on the large meridian expansion stator is worth studying. The flow and heat transfer characteristics of the downstream large meridional expansion turbine stator were studied by comparing the tip leakage flow of 1.5-stage shrouded and unshrouded turbines using a three-dimensional Reynolds-Averaged Navier-Stokes (RANS) solver for viscous turbulent flows. Validation studies were performed to investigate the aerodynamics and heat transfer prediction ability of the shear stress transport (SST) turbulence model. The influence of different tip clearances of the rotor including unshrouded blade heights of 0%, 1% and 5% and a 1% shrouded blade height were investigated through numerical simulation. The results showed that the upper passage vortex separation was more serious and the separation, and attachment point of horseshoe vortex in the pressure side were significantly more advanced than that of non-expansion turbines. The tip leakage vortex obviously increased the negative incidence angle at the downstream inlet. Furthermore, the strength of the high heat transfer zone on the suction surface of the downstream stator was significantly increased, while that of the shrouded rotor decreased.
Assessment of Productivity and Economic Viability of Combined Food and Energy (CFE) Production System in Denmark
Authors: Xu Ying , Lehmann Lisa Mølgaard , García de Jalón Silvestre , Ghaley Bhim Bahadur
Published: January 2019

Agro-ecosystems for integrated food, fodder, and biomass production can contribute to achieving European Union goals to increase renewable energy sources and reduce greenhouse gas emissions. The study objective was to evaluate the productivity and economic returns from a combined food and energy (CFE) system compared to sole winter wheat and sole short rotation woody crop (SRWC) production. Two excel-based models viz. Yield-SAFE and Farm-SAFE, were used to simulate agronomic productivity and economic assessment respectively. Yield-SAFE was calibrated and validated with measured data from CFE from 1996–2016. When compared over temporal scale of 21 years, CFE systems with 150–200 m alley width had the highest net present value (NPV) followed by 100 m, 50 m, sole winter wheat and sole SRWC, indicating higher profitability of CFE systems. Sensitivity analysis of NPV with ±10% yield fluctuations, and with 0–10% discount rate, demonstrated that CFE systems was more profitable than sole crops, indicating higher resilience in CFE systems. LER in CFE ranged from 1.14–1.34 indicative of higher productivity of CFE systems compared to component monocultures. Hence, the study has demonstrated that the productivity and the economic viability of CFE systems, were higher than sole crops, for informed decision making by farm managers and policy makers to contribute to renewable energy biomass production and to mitigate the impending adverse climate change effects on agricultural production.
An Ant Colony Optimized MPPT for Standalone Hybrid PV-Wind Power System with Single Cuk Converter
Authors: Priyadarshi Neeraj , Ramachandaramurthy Vigna , Padmanaban Sanjeevikumar , Azam Farooque
Published: January 2019

This research work explains the practical realization of hybrid solar wind-based standalone power system with maximum power point tracker (MPPT) to produce electrical power in rural places (residential applications). The wind inspired Ant Colony Optimization (ACO)-based MPPT algorithm is employed for the purpose of fast and accurate tracking power from wind energy system. Fuzzy Logic Control (FLC) inverter controlling strategy is adopted in this presented work compared to classical proportional-integral (PI) control. Moreover, single Cuk converter is operated as impedance power adapter to execute MPPT functioning. Here, ACO-based MPPT has been implemented with no voltage and current extra circuit requirement compared to existing evolutionary algorithms single cuk converter is employed to improve conversion efficiency of converter by maximizing power stages. DC-link voltage can be regulated by placing Cuk converter Permanent Magnet Synchronous Generator (PMSG) linked rectifier and inverter. The proposed MPPT method is responsible for rapid battery charging and gives power dispersion of battery for hybrid PV-Wind system. ACO-based MPPT provides seven times faster convergence compared to the particle swarm optimization (PSO) algorithm for achievement of maximum power point (MPP) and tracking efficiency. Satisfactory practical results have been realized using the dSPACE (DS1104) platform that justify the superiority of proposed algorithms designed under various operating situations.
Stocks and Stoichiometry of Soil Organic Carbon, Total Nitrogen, and Total Phosphorus after Vegetation Restoration in the Loess Hilly Region, China
Authors: Xu Hongwei , Qu Qing , Li Peng , Guo Ziqi , Wulan Entemake , Xue Sha
Published: January 2019

The Loess Plateau is an important region for vegetation restoration in China; however, changes in soil organic carbon (SOC), soil nutrients, and stoichiometry after restoration in this vulnerable ecoregion are not well understood. Typical restoration types, including orchardland, grassland, shrubland, and forestland, were chosen to examine changes in the stocks and stoichiometry of SOC, soil total nitrogen (TN), and soil total phosphorus (TP) at different soil depths and recovery times. Results showed that SOC stocks first increased and then stabilized in orchardland, grassland, and shrubland at 0–30 cm depths, while in forestland, SOC stocks gradually increased. Soil TN stocks first increased and then decreased in orchardland, shrubland, and forestland with restoration age at 0–30 cm depths, while soil TP stocks showed little variation between restoration types; at the same time, the overall C:N, C:P, and N:P ratios increased with restoration age. In the later stages of restoration, the stocks of SOC and soil TN at 0–30 cm soil depths were still lower than those in natural grassland and natural forest. Additionally, the SOC, soil TN, and soil TP stocks and the C:N, C:P, and N:P ratios decreased with soil depth. The forestland had the highest rate of change in SOC and soil TN stocks, at 0–10 cm soil depth. These results indicate a complex response of SOC, soil TN, and soil TP stocks and stoichiometry to vegetation restoration, which could have important implications for understanding C, N, and P changes and nutrient limitations after vegetation restoration.
Evolution of the Pattern of Spatial Expansion of Urban Land Use in the Poyang Lake Ecological Economic Zone
Authors: Zhong Yang , Lin Aiwen , Zhou Zhigao
Published: January 2019

To grasp the evolutionary characteristics and regularity of urban land expansion patterns in the Poyang Lake Ecological Economic Zone, this study, based on nighttime lighting data, uses the Landsat series satellite simultaneous data and cluster analysis to correct the Defense Meteorological Satellite Program–Operational Linescan System (DMSP-OLS) nighttime lighting data and then uses the auxiliary data-based comparison method to determine the threshold for extracting the urban built-up area. Based on this threshold, a total of eight typical landscape pattern indicators, including landscape total area, total patches number, patches density, maximum patches index, and agglomeration index, etc., are selected. Next, the landscape spatial pattern analysis method and standard deviation ellipse method are used. The results show the following: (1) In 1992–2013, urbanization in the Poyang Lake Ecological Economic Zone expanded rapidly. The urban built-up area increased by 8.13 times, the number of plaques increased by 1.5 times, and the shape complexity of landscape plaques gradually increased. There is a large correlation between the changes in the total boundary length, and the average boundary density, the average annual growth rate of the two is 21.33% and 17.45%. (2) The two indicators of maximum plaque index and aggregation index show a downward trend year by year. However, there are some fluctuations and irregularities in the evolution of the total landscape area, total plaque number and plaque density. (3) The long axis and the short axis of the standard deviation ellipse of the Poyang Lake Ecological Economic Zone show small variation during the inspection period and generally have an elliptical shape. The movement of the center of gravity is mainly from the southwest to the northeast, but the migration of the center of gravity is relatively small. Based on this, this paper proposes three countermeasures and suggestions as a guide to promote the optimization and development of the spatial expansion pattern of the Poyang Lake eco-economic zone.
Saltwater Intrusion Function and Preliminary Application in the Yangtze River Estuary, China
Authors: Xu Zhi , Ma Jing , Hu Yajie
Published: January 2019

More attention has been paid to saltwater-intrusion-related problems in recent years. In this research study, a saltwater intrusion function in the Yangtze River Estuary (YRE) was constructed based on the theory of the interactions between energy accumulation and impedance. A MIKE21 model was used to simulate the hydrodynamics of the YRE. Then, through the analysis of the relationships between the river discharge conditions, tidal ranges, and saltwater intrusion, it was determined that, under certain river discharge conditions, the tidal ranges and salinity levels at the stations in the southern branch (SB) of the YRE conformed to S-shaped curve characteristics. Also, the tidal ranges and salinity excessive area rate (SEAR) displayed similar characteristics. Furthermore, the river discharge conditions were also found to match the S-curve characteristics between the two aforementioned relationship features. Therefore, the saltwater intrusion function of the YRE was constructed based on the previously mentioned development rules. Also, the applied quantification methods were elaborated, and the values of the parameters were determined. As a result, the critical river discharge (more than 10,000 m3/s) was obtained, which could withstand large-scale saltwater intrusions. When the river discharge was greater than 30,000 m3/s, the area was considered to be basically without salt water intrusions, and the estuarine ecology was in an optimal state. The saltwater intrusion losses from 2005 to 2015 are also calculated. These findings have important reference value for water dispatching of the YRE in the dry season.
The Relationship between Nkx2.1 and DNA Oxidative Damage Repair in Nickel Smelting Workers: Jinchang Cohort Study
Authors: Cheng Zhiyuan , Cheng Ning , Shi Dian , Ren Xiaoyu , Gan Ting , Bai Yana , Yang Kehu
Published: January 2019

Background: Occupational nickel exposure can cause DNA oxidative damage and influence DNA repair. However, the underlying mechanism of nickel-induced high-risk of lung cancer has not been fully understood. Our study aims to evaluate whether the nickel-induced oxidative damage and DNA repair were correlated with the alterations in Smad2 phosphorylation status and Nkx2.1 expression levels, which has been considered as the lung cancer initiation gene. Methods: 140 nickel smelters and 140 age-matched administrative officers were randomly stratified by service length from Jinchang Cohort. Canonical regression, χ2 test, Spearman correlation etc. were used to evaluate the association among service length, MDA, 8-OHdG, hOGG1, PARP, pSmad2, and Nkx2.1. Results: The concentrations of MDA, PARP, pSmad2, and Nkx2.1 significantly increased. Nkx2.1 (rs = 0.312, p < 0.001) and Smad2 phosphorylation levels (rs = 0.232, p = 0.006) were positively correlated with the employment length in nickel smelters, which was not observed in the administrative officer group. Also, elevation of Nkx2.1 expression was positively correlated with service length, 8-OHdG, PARP, hOGG1 and pSmad2 levels in nickel smelters. Conclusions: Occupational nickel exposure could increase the expression of Nkx2.1 and pSmad2, which correlated with the nickel-induced oxidative damage and DNA repair change.
A Toxicogenomic Approach Reveals a Novel Gene Regulatory Network Active in In Vitro and In Vivo Models of Thyroid Carcinogenesis
Authors: Reale Carla , Russo Filomena , Credendino Sara Carmela , Cuomo Danila , De Vita Gabriella , Mallardo Massimo , Pennino Francesca , Porreca Immacolata , Triassi Maria , De Felice Mario , Ambrosino Concetta
Published: January 2019

Epidemiological and experimental studies emphasize the link between environmental chemicals exposure and thyroid cancer. However, this association is strongly debated and the mechanisms of action of environmental thyroid carcinogens still need to be identified. The analysis of in vitro transcriptomic data developed to investigate the effects of chlorpyrifos on immortalized thyrocytes highlighted the impaired expression of genes involved in endodermal carcinogenesis. This endodermal carcinogenic gene-network (ECGN, including Zfp36l2, Dmbt1, Ddit4), was validated in cellular and mouse models of thyroid carcinogenesis, characterized by the constitutive activation of the mitogen-activated protein kinase (MAPK) pathway and in immortalized thyrocytes exposed to tetrachlorodibenzo-p-dioxin (TCDD) and chlorpyrifos (CPF). The mRNA levels of Zfp36l2, Dmbt1 and Ddit4 were increased in models characterized by MAPK activation or following TCDD exposure, whereas they were inhibited by CPF exposure. Overall, the ECGN transcripts identify a novel gene-regulatory network associated with thyroid carcinogenesis promoted by genetic mutation or by environmental carcinogens. The latter have opposite effects on the modulation of the ECGN transcripts according to their mechanisms of action in promoting carcinogenesis. Therefore, the analyses of ECGN might be helpful in discriminating compounds that promote cellular survival associated or not to proliferation of thyrocytes.
Impact of Land Use/Cover Change on Yangtze River Delta Urban Agglomeration Ecosystem Services Value: Temporal-Spatial Patterns and Cold/Hot Spots Ecosystem Services Value Change Brought by Urbanization
Authors: Li Zhigang , Sun Zishu , Tian Yangjie , Zhong Jialong , Yang Wunian
Published: January 2019

Land use/cover change (LUCC) from increased urbanization significantly impacts regional ecosystem services. Based on a cold/hot spots analysis, this paper used grain yield, food prices, price index statistics, and a land use thematic map to study the impact of LUCC on four ecosystem services values (ESVs) in the Yangtze River Delta urban agglomeration, and determine the spatial differences resulting from the rapid urbanization LUCC. The correlation between the four ecosystem services was then studied and sensitivity analyses conducted to investigate whether any changes in the ESVs could lead to unacceptable unit value transfer uncertainties. It was found that most urban land was converted from farmland, and that before 2000, the total ESVs and the regulating services values (RSVs) increased significantly, after which it declined, the provisioning services values (PSVs) declined year on year, the habitat services value (HSV) and cultural and amenity services value (CSV) declined sharply after 2000, and the spatial distribution of the four ESVs were significantly different. Over time, it was found that the hot spots were shrinking and the cold spots were spreading. The provisioning services were found to be negatively correlated with habitat services and cultural and amenity services, the regulating services were weakly positively correlated with the provisioning services and significantly positively correlated with the habitat services and cultural and amenity services, and the habitat services were significantly positively correlated with cultural and amenity services. In the Yangtze River Delta urban agglomeration, the water area is the most important for the total ESVs, followed by non-bush forest. Paddy field is ranked third. Dryland, bush, grassland, and wetland are less important. The importance of barren land is almost zero. This research provides the government with a scientific basis from which to formulate spatial planning and environmental protection policies for ecological sustainable development in the Yangtze River Delta urban agglomeration.
Study on the Status of Health Service Utilization among 3–5 Years Old Left-Behind Children in Poor Rural Areas of Hunan Province, China: A Cross-Sectional Survey
Authors: Ouyang Yufeng , Zou Jiaojiao , Ji Meimei , Zhang Yefu , Yuan Tong , Yang Lina , Lin Qian
Published: January 2019

The left-behind children (LBC) in China generally refer to children who remain in rural regions under the care of kin members while their parents migrate to urban areas. Due to some reasons, e.g., poverty, poor transportation conditions, lack of health resources, and preschool child care, it is hard for preschool-aged rural LBC to obtain essential health services. Random cluster sampling was used to recruit the caregivers and all the 3–5-year-old LBC in two rural counties in Hunan Province. A questionnaire was used to collect data on LBC demographics via face-to-face interviews with the caregivers. Health service needs were evaluated by the two-week prevalence rate, while health service utilization was measured by the two-week physician visit rate. Of the 559 respondents in the study, the two-week prevalence rate was 44.2% and the two-week physician visits rate was 48.6%. Nearly half of the sick children did not go to a hospital, 45.7% self-treated, and 5.3% did not take any treatment. The utilization rates of “health check,” “eye exam,” and “hearing screening for 3-year-olds” were extremely low (57.3%, 29.3%, 18.7%). The utilization rate of health services for preschool LBC in poor rural areas was extremely low, which can affect the normal growth and development of children.
Nesfatin-1 and Vaspin as Potential Novel Biomarkers for the Prediction and Early Diagnosis of Gestational Diabetes Mellitus
Authors: Mierzyński Radzisław , Poniedziałek-Czajkowska Elżbieta , Dłuski Dominik , Patro-Małysza Jolanta , Kimber-Trojnar Żaneta , Majsterek Maciej , Leszczyńska-Gorzelak Bożena
Published: January 2019

Gestational diabetes mellitus (GDM) is considered to be one of the most frequent medical complication observed among pregnant women. The role of adipokines in the pathogenesis of GDM remains strictly unknown. Different adipokines have been studied throughout gestation, and they have been proposed as biomarkers of GDM and other pregnancy-related complications; however, there is no biomarker reported for GDM screening at present. The aim of this study was to evaluate serum nesfatin-1 and vaspin levels in GDM and non-GDM women, to characterize the correlation between these adipokines, and to assess the potential role of circulating adipokines in the prediction of risk of gestational diabetes mellitus. Serum concentrations of nesfatin-1 and vaspin were measured in 153 women with GDM, and in 84 patients with uncomplicated pregnancy by enzyme-linked immunosorbent assay (ELISA) kits, according to the manufacturer’s instructions. Circulating levels of nesfatin-1 and vaspin were significantly lower in the GDM group than in the control group. Nesfatin-1 levels were negatively correlated with vaspin levels. The results of this study point out the possible role of nesfatin-1 and vaspin as potential novel biomarkers for the prediction and early diagnosis of GDM. Further studies are necessary to evaluate the influence of nesfatin-1 and vaspin on glucose metabolism in the early stages of GDM.
Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Reduces Oxidative and Mechanical Stress-Evoked Matrix Degradation in Chondrifying Cell Cultures
Authors: Szentléleky Eszter , Szegeczki Vince , Karanyicz Edina , Hajdú Tibor , Tamás Andrea , Tóth Gábor , Zákány Róza , Reglődi Dóra , Juhász Tamás
Published: January 2019

Pituitary adenylate cyclase activating polypeptide (PACAP) is an endogenous neuropeptide also secreted by non-neural cells, including chondrocytes. PACAP signaling is involved in the regulation of chondrogenesis, but little is known about its connection to matrix turnover during cartilage formation and under cellular stress in developing cartilage. We found that the expression and activity of hyaluronidases (Hyals), matrix metalloproteinases (MMP), and aggrecanase were permanent during the course of chondrogenesis in primary chicken micromass cell cultures, although protein levels changed daily, along with moderate and relatively constant enzymatic activity. Next, we investigated whether PACAP influences matrix destructing enzyme activity during oxidative and mechanical stress in chondrogenic cells. Exogenous PACAP lowered Hyals and aggrecanase expression and activity during cellular stress. Expression and activation of the majority of cartilage matrix specific MMPs such as MMP1, MMP7, MMP8, and MMP13, were also decreased by PACAP addition upon oxidative and mechanical stress, while the activity of MMP9 seemed not to be influenced by the neuropeptide. These results suggest that application of PACAP can help to preserve the integrity of the newly synthetized cartilage matrix via signaling mechanisms, which ultimately inhibit the activity of matrix destroying enzymes under cellular stress. It implies the prospect that application of PACAP can ameliorate articular cartilage destruction in joint diseases.
Therapeutic Targets for Bone and Soft-Tissue Sarcomas
Authors: Miwa Shinji , Yamamoto Norio , Hayashi Katsuhiro , Takeuchi Akihiko , Igarashi Kentaro , Tsuchiya Hiroyuki
Published: January 2019

Due to the rarity and heterogeneity of bone and soft-tissue sarcomas, investigation into molecular targets and new treatments has been particularly challenging. Although intensive chemotherapy and establishment of surgical procedures have improved the outcomes of patients with sarcoma, the curative rate of recurrent and metastatic sarcomas is still not satisfactory. Recent basic science research has revealed some of the mechanisms of progression and metastasis of malignancies including proliferation, apoptosis, angiogenesis, tumor microenvironment, migration, invasion, and regulation of antitumor immune systems. Based on these basic studies, new anticancer drugs, including pazopanib, trabectedin, eribulin, and immune checkpoint inhibitors have been developed and the efficacies and safety of the new drugs have been assessed by clinical trials. This review summarizes new molecular therapeutic targets and advances in the treatment for bone and soft tissue sarcomas.
Bringing the Illusion of Reality Inside Museums—A Methodological Proposal for an Advanced Museology Using Holographic Showcases
Authors: Pietroni Eva , Ferdani Daniele , Forlani Massimiliano , Pagano Alfonsina , Rufa Claudio
Published: January 2019

The basic idea of a hologram is an apparition of something that does not exist but appears as if it was just in front of our eyes. These illusion techniques were invented a long time ago. The philosopher and alchemist Giovanni Battista della Porta invented an effect that was later developed and brought to fame by Prof. J. H. Pepper (1821–1900) and applied in theatrical performances. The innovation nowadays consists in the adopted technology to produce them. Taking advantage of the available digital technologies, the challenge we are going to discuss is using holograms in the museum context, inside showcases, to realize a new form of scenography and dramaturgy around the exhibited objects. Case studies will be presented, with a detailed analysis of the EU project CEMEC (Connecting Early Medieval European Collections), where holographic showcases have been designed, built and experimented in EU museums. In this case, the coexistence in the same space of the real artifact and the virtual contents, and interior setup of the showcase, its dynamic lighting system, the script and the sound, converge to create an expressive unity. The reconstruction of sensory and symbolic dimensions that are ‘beyond’ any museum object can take the visitor in the middle of a lively and powerful experience with such technology, and represents an advancement in the museological sector. User experience results and a list of best practices will be presented in the second part of the paper, out of the tests and research activities conducted in these three years of the project.
Measurement of the Beam Energy Distribution of a Medical Cyclotron with a Multi-Leaf Faraday Cup
Authors: Nesteruk Konrad P. , Ramseyer Luca , Carzaniga Tommaso S. , Braccini Saverio
Published: January 2019

Accurate knowledge of the beam energy distribution is crucial for particle accelerators, compact medical cyclotrons for the production of radioisotopes in particular. For this purpose, a compact instrument was developed, based on a multi-leaf Faraday cup made of thin aluminum foils interleaved with plastic absorbers. The protons stopping in the aluminum foils produce a measurable current that is used to determine the range distribution of the proton beam. On the basis of the proton range distribution, the beam energy distribution is assessed by means of stopping-power Monte Carlo simulations. In this paper, we report on the design, construction, and testing of this apparatus, as well as on the first measurements performed with the IBA Cyclone 18-MeV medical cyclotron in operation at the Bern University Hospital.
Low Volume, Home-Based Weighted Step Exercise Training Can Improve Lower Limb Muscle Power and Functional Ability in Community-Dwelling Older Women
Authors: Mair Jacqueline L. , De Vito Giuseppe , Boreham Colin A.
Published: January 2019

Stepping exercise can be used as a scalable form of high intensity exercise to enhance important aspects of physical fitness in older populations. The addition of supplementary weights increases the resistive element of stepping, with the potential for training improvements in muscular strength, power, and functional abilities alongside other fitness outcomes. The aim of this study was to evaluate the effects of a low-volume, home-based weighted step exercise programme on muscular strength, power, and functional ability in previously inactive community-dwelling older women. Eleven participants, aged between 65–74 years, independently completed a six-week individualised and progressive step exercise training programme wearing a weighted vest. Knee extensor strength, lower limb power output, and physical function using a battery of functional tests were measured at baseline, following a 6-week control period, and again following the 6-week training programme. Following training, lower limb power output improved by 10–11% (p < 0.05) and was accompanied by a corresponding 9% (p < 0.01) improvement in stair climb time and 10% (p < 0.01) improvement in normalised stair climbing power, highlighting the beneficial effects of weighted stepping for transferable improvements in functional fitness. The magnitude of observed training improvements suggest that weighted step training has the potential to prolong independence and prevent age-related health conditions such as sarcopenia.
Laboratory and Numerical Investigation of Microwave Heating Properties of Asphalt Mixture
Authors: Wang Haopeng , Zhang Yue , Zhang Yi , Feng Shuyin , Lu Guoyang , Cao Lintao
Published: January 2019

Microwave heating is an encouraging heating technology for the maintenance, recycling, and deicing of asphalt pavement. To investigate the microwave heating properties of asphalt mixture, laboratory tests and numerical simulations were done and compared. Two types of Stone Mastic Asphalt (SMA) mixture samples (with basalt aggregates and steel slag aggregates) were heated using a microwave oven for different times. Numerical simulation models of microwave heating of asphalt mixture were developed with finite element software COMSOL Multiphysics. The main thermal and electromagnetic properties of asphalt mixture, served as the model input parameters, were measured through a series of laboratory tests. Both laboratory-measured and numerical simulated surface temperatures were recorded and analyzed. Results show that the replacement of basalt aggregates with steel slag aggregates can significantly increase the microwave heating efficiency of asphalt mixture. Numerical simulation results have a good correlation with laboratory test results. It is feasible to use the developed model coupling electromagnetic waves with heat transfer to simulate the microwave heating process of asphalt mixture.
Chlorogenic Acid Entrapped in Hybrid Materials with High PEG Content: A Strategy to Obtain Antioxidant Functionalized Biomaterials?
Authors: Catauro Michelina , Barrino Federico , Dal Poggetto Giovanni , Crescente Giuseppina , Piccolella Simona , Pacifico Severina
Published: January 2019

The formation of pro-oxidant species after implantation of biomaterials could be responsible for the failure of the implant itself, because of oxidative stress-induced damage. In this work, the SiO2/polyethylene glycol (PEG)/chlorogenic acid (CGA) hybrids synthesized by the sol–gel method with 50 wt% of the polymer and different amounts of CGA (5, 10, 15 and 20 wt%) were studied. The hybrids soaked in simulated body fluid (SBF) showed the formation of hydroxyapatite layers on their surface, suggesting that the hybrids are bioactive. Their radical scavenging capacity towards DPPH· and ABTS·+ (2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), evaluated at three different doses (0.5, 1 and 2 mg), showed probe- and dose-dependent behavior. In addition, the antioxidant properties of CGA were not affected by the presence of high amounts of the polymer. The in vitro biocompatibility in three cell lines (NIH 3T3, HaCaT and SH-SY5Y) was assessed by using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Apart from SH-SY5Y, the cell viability—expressed as mitochondrial redox activity percentage of cells directly exposed to powders—and morphology was not affected, suggesting that the hybrids have the ability to interfere and act selectively against tumor cells. The antibacterial properties of the different materials against Escherichia coli and Enterococcus faecalis were affected by different amounts of the natural antioxidant component.
Global Optimization for Quasi-Noncyclic Relatively Nonexpansive Mappings with Application to Analytic Complex Functions
Authors: Kumam Poom , Mongkolkeha Chirasak
Published: January 2019

The purpose of this article is to resolve a global optimization problem for quasi-noncyclic relatively nonexpansive mappings by giving an algorithm that determines an optimal approximate solution of the following minimization problem, min x ∈ A d ( x , T x ) , min y ∈ B d ( y , T y ) and min ( x , y ) ∈ A × B d ( x , y ) ; also, we provide some illustrative examples to support our results. As an application, the existence of a solution of the analytic complex function is discussed.
Capillasterin A, a Novel Pyrano[2,3-f]chromene from the Australian Crinoid Capillaster multiradiatus
Authors: Lum Kah , Carroll Anthony , Ekins Merrick , Read Silven , Haq Zahra , Tietjen Ian , St John James , Davis Rohan
Published: January 2019

Capillasterin A (1), a novel pyrano[2,3-f]chromene, together with seven known naphthopyrones including comaparvin (2), TMC-256C1 (3), 6-methoxycomaparvin-5- methyl ether (4), 5,8-dihydroxy-6-methoxy-2-propyl-4H-naphtho[2,3-b]pyran-4-one (5), 5,8-dihydroxy-6,10-dimethoxy-2-propyl-4H-naphtho[2,3-b]pyran-4-one (6), TMC-256A1 (7) and 6-methoxycomaparvin (8) were isolated from an EtOH/H2O extract from the Australian crinoid Capillaster multiradiatus. The structures of all the compounds were determined by detailed spectroscopic (1D/2D NMR and MS) data analysis. This is the first report of a natural product that contains the pyrano[2,3-f]chromene skeleton. Compounds 2–6 were observed to display moderate inhibition of in vitro HIV-1 replication in a T cell line with EC50 values ranging from 7.5 to 25.5 µM without concomitant cytotoxicity.
Physical Modelling of the Ball-Rolling Processes
Authors: Pater Zbigniew , Tomczak Janusz , Wójcik Łukasz , Bulzak Tomasz
Published: January 2019

The objective of the article was to present the state of the problem of physical modelling of the hot-working processes with plasticine as the model material. It was stated that the aforementioned method can prove helpful in analyzing complex plastic forming processes such as cross rolling and helical rolling of balls. In order to confirm this hypothesis, an attempt at forming steel balls with diameters of 40 mm (cross rolling) and 57 mm (helical rolling) under laboratory conditions was made. Further on, these processes were conducted in model form using special model rolling mills and 3D printed acrylonitrile butadiene styrene (ABS) tools. The comparison of the test results regarding shape and manufacturing accuracy, as well as force parameters, confirmed the validity of using physical modelling in the investigation of the process of cross rolling and helical rolling of balls.
Experimental Study on Cross Wedge Rolling of 21-4N Heat Resistant Steel
Authors: Zheng Zhenhua
Published: January 2019

21-4N is a typical heat resistant steel used as the material of exhaust valves. The application of cross wedge rolling to manufacture preform for valves has the advantages of higher efficiency and better quality. In this study, cross wedge rolling of 21-4N with an area reduction ratio of 65% was experimentally studied. Twelve groups of different tool parameters of cross wedge rolling tests were carried out. It is found that a larger stretching angle or a smaller forming angle is better for surface quality. When the stretching angle is 7° and the forming angle is less than 25°, a smooth surface can be obtained. Furthermore, the influence of stretching angle on central quality is not obvious, but a greater forming angle is prone to get a better central quality. When the forming angle is larger than 30°, no central damage was found in the workpieces. Considering the balance of surface quality and central quality, a stretching angle of 7° and a forming angle of 30° is a suitable combination for cross wedge rolling of 21-4N with an area reduction ratio of 65%.
Addendum: de Souza Mesquita, L.M., et al. Modulatory Effect of Polyphenolic Compounds from the Mangrove Tree Rhizophora mangle L. on Non-Alcoholic Fatty Liver Disease and Insulin Resistance in High-Fat Diet Obese Mice. Molecules, 2018, 23, 2114
Authors: De Souza Mesquita Leonardo Mendes , Caria Cíntia Rabelo e Paiva , Santos Paola Souza , Ruy Caio Cesar , Da Silva Lima Natalia , Moreira Débora Kono Taketa , Da Rocha Claudia Quintino , Murador Daniella Carisa , De Rosso Veridiana Vera , Gambero Alessandra , Vilegas Wagner
Published: January 2019

The authors wish to add the process number in the Acknowledgments section in this paper [...]
Molecular Identification and Targeted Quantitative Analysis of Medicinal Materials from Uncaria Species by DNA Barcoding and LC-MS/MS
Authors: Wei Shugen , Luo Zuliang , Cui Shengrong , Qiao Jing , Zhang Zhonglian , Zhang Lixia , Fu Jine , Ma Xiaojun
Published: January 2019

The genus Uncaria is an important source of traditional Chinese medicines with multiple therapeutic effects. The identification of the correct species and accurate determination of the contents of bioactive constituents are important for quality control of Uncaria medicinal materials. Here, an integrated evaluation system based on DNA barcoding for species identification and quantitative analysis by LC-MS/MS has been established. DNA barcoding based on the ITS2 barcode region showed sufficient discriminatory power to precisely identify 24 samples from seven Uncaria species. The length of the 24 ITS2 sequences of Uncaria samples is 227 bp, and 17 variation sites were detected. Additionally, the results of qualitative and quantitative chemical analyses by LC-MS/MS indicated that the chemical compositions of all Uncaria samples were similar; while their contents of targeted alkaloids in samples from different species and origin areas were different. The contents of rhynchophylline (RC) and isorhynchophylline (IRC) were 2.9–1612 mg/kg and 2.60–1299 mg/kg in all tested samples, respectively. This study concludes that DNA barcoding should be used as the first screening step for Uncaria medicinal materials. Then, integration of DNA barcoding with chemical analyses should be applied in quality control of Uncaria medicinal materials in the medicinal industry.
Effect of Wakame and Carob Pod Snacks on Non-Alcoholic Fatty Liver Disease
Authors: Rico Daniel , Martin-Diana Ana Belén , Lasa Arrate , Aguirre Leixuri , Milton-Laskibar Iñaki , De Luis Daniel Antonio , Miranda Jonatan
Published: January 2019

Snacks combining different functional ingredients could represent a useful therapeutic strategy against NAFLD. The present study aimed to analyze the effect of two snack formulations based on carob and wakame flour in the treatment for NAFLD in rats. For this purpose, metabolic syndrome was induced in 50 adult rats by a high-fat high-fructose diet over eight weeks. After this period, rats were fed either normal calorie diets supplemented or not with snack A (1/50 wakame/carob pod) and snack B (1/5 wakame/carob pod) for four additional weeks. After sacrifice, liver composition and serum parameters were analyzed. Different pathways of triacylglycerol metabolism in liver were studied including fatty acid oxidation, fatty acid synthesis, triglyceride assembly and release, fatty acid uptake and glucose uptake. Oxidative stress was also measured. Snack treatment, and mainly B snack, reduced liver triacylglycerol levels by increasing fat oxidation. Moreover, this snack reduced oxidative stress. Therefore, this snack formulation could represent an interesting tool useful for fatty liver treatment.
Co-Loading of Ascorbic Acid and Tocopherol in Eudragit-Nutriosomes to Counteract Intestinal Oxidative Stress
Authors: Rezvani Maryam , Manca Maria Letizia , Caddeo Carla , Escribano-Ferrer Elvira , Carbone Claudia , Peris José Esteban , Usach Iris , Diez-Sales Octavio , Fadda Anna Maria , Manconi Maria
Published: January 2019

The present study aimed at developing a new vesicular formulation capable of promoting the protective effect of ascorbic acid and tocopherol against intestinal oxidative stress damage, and their efficacy in intestinal wound healing upon oral administration. A pH-dependent copolymer (Eudragit® L100), a water-soluble prebiotic fibre (Nutriose® FM06), a phospholipid mixture (Lipoid S75), and two natural antioxidants (ascorbic acid and tocopherol) were combined to fabricate eudragit-nutriosomes by a simple, solvent-free procedure. The vesicles were spherical and oligolamellar, with some multicompartment structures in Eudragit-nutriosomes, small in size (~100 nm), with highly negative zeta potential. The effect of Eudragit® and Nutriose® on the stability on storage and in simulated gastrointestinal fluids were confirmed by the Turbiscan® technology and in vitro studies, respectively. Eudragit-nutriosomes exhibited a protective effect against H2O2-induced oxidative stress, and a proliferative effect in Caco-2 cells, as they provided the closure of the scratched area after 96 h of incubation.
Functional Characterization of microRNA171 Family in Tomato
Authors: Kravchik Michael , Stav Ran , Belausov Eduard , Arazi Tzahi
Published: January 2019

Deeply conserved plant microRNAs (miRNAs) function as pivotal regulators of development. Nevertheless, in the model crop Solanum lycopersicum (tomato) several conserved miRNAs are still poorly annotated and knowledge about their functions is lacking. Here, the tomato miR171 family was functionally analyzed. We found that the tomato genome contains at least 11 SlMIR171 genes that are differentially expressed along tomato development. Downregulation of sly-miR171 in tomato was successfully achieved by transgenic expression of a short tandem target mimic construct (STTM171). Consequently, sly-miR171-targeted mRNAs were upregulated in the silenced plants. Target upregulation was associated with irregular compound leaf development and an increase in the number of axillary branches. A prominent phenotype of STTM171 expressing plants was their male sterility due to a production of a low number of malformed and nonviable pollen. We showed that sly-miR171 was expressed in anthers along microsporogenesis and significantly silenced upon STTM171 expression. Sly-miR171-silenced anthers showed delayed tapetum ontogenesis and reduced callose deposition around the tetrads, both of which together or separately can impair pollen development. Collectively, our results show that sly-miR171 is involved in the regulation of anther development as well as shoot branching and compound leaf morphogenesis.
Multi-Material Additive Manufacturing of Sustainable Innovative Materials and Structures
Authors: Singh Rupinder , Kumar Ranvijay , Farina Ilenia , Colangelo Francesco , Feo Luciano , Fraternali Fernando
Published: January 2019

This paper highlights the multi-material additive manufacturing (AM) route for manufacturing of innovative materials and structures. Three different recycled thermoplastics, namely acrylonitrile butadiene styrene (ABS), polylactic acid (PLA), and high impact polystyrene (HIPS) (with different Young’s modulus, glass transition temperature, rheological properties), have been selected (as a case study) for multi-material AM. The functional prototypes have been printed on fused deposition modelling (FDM) setup as tensile specimens (as per ASTM D638 type-IV standard) with different combinations of top, middle, and bottom layers (of ABS/PLA/HIPS), at different printing speed and infill percentage density. The specimens were subjected to thermal (glass transition temperature and heat capacity) and mechanical testing (peak load, peak strength, peak elongation, percentage elongation at peak, and Young’s modulus) to ascertain their suitability in load-bearing structures, and the fabrication of functional prototypes of mechanical meta-materials. The results have been supported by photomicrographs to observe the microstructure of the analyzed multi-materials.
Fabrication of 3D PDMS Microchannels of Adjustable Cross-Sections via Versatile Gel Templates
Authors: Ng Pui Fai , Lee Ka I , Yang Mo , Fei Bin
Published: January 2019

Flexible gel fibers with high stretchability were synthesized from physically cross-linked agar and covalently cross-linked polyacrylamide networks. Such gel material can withstand the temperature required for thermal curing of polydimethylsiloxane (PDMS), when the water in the gel was partially replaced with ethylene glycol. This gel template supported thermal replica molding of PDMS to produce high quality microchannels. Microchannels with different cross sections and representative 3D structures, including bifurcating junction, helical and weave networks, were smoothly fabricated, based on the versatile manipulation of gel templates. This gel material was confirmed as a flexible and reliable template in fabricating 3D microfluidic channels for potential devices.
Model for Thin Layer Drying of Lemongrass (Cymbopogon citratus) by Hot Air
Authors: Nguyen Thi Van Linh , Nguyen My Duyen , Nguyen Duy Chinh , Bach Long Giang , Lam Tri Duc
Published: January 2019

Lemongrass is a plant that contains aromatic compounds (myrcene and limonene), powerful deodorants, and antimicrobial compounds (citral and geraniol). Identifying a suitable drying model for the material is crucial for establishing an initial step for the development of dried products. Convection drying is a commonly used drying method that could extend the shelf life of the product. In this study, a suitable kinetic model for the drying process was determined by fitting moisture data corresponding to four different temperature levels: 50, 55, 60 and 65 °C. In addition, the effect of drying temperature on the moisture removal rate, the effective diffusion coefficient and activation energy were also estimated. The results showed that time for moisture removal increases proportionally with the air-drying temperature, and that the Weibull model is the most suitable model for describing the drying process. The effective diffusion coefficient ranges from 7.64 × 10−11 m2/s to 1.48 × 10−10 m2/s and the activation energy was 38.34 kJ/mol. The activation energy for lemongrass evaporation is relatively high, suggesting that more energy is needed to separate moisture from the material by drying.
Extended Dependence of the Hydrological Regime on the Land Cover Change in the Three-North Region of China: An Evaluation under Future Climate Conditions
Authors: Yao Yi , Xie Xianhong , Meng Shanshan , Zhu Bowen , Zhang Kang , Wang Yibing
Published: January 2019

The hydrological regime in arid and semi-arid regions is quite sensitive to climate and land cover changes (LCC). The Three-North region (TNR) in China experiences diverse climate conditions, from arid to humid zones. In this region, substantial LCC has occurred over the past decades due to ecological restoration programs and urban expansion. At a regional scale, the hydrological effects of LCC have been demonstrated to be less observable than the effects of climate change, but it is unclear whether or not the effects of LCC may be intensified by future climate conditions. In this study, we employed remote sensing datasets and a macro-scale hydrological modeling to identify the dependence of the future hydrological regime of the TNR on past LCC. The hydrological effects over the period from 2020–2099 were evaluated based on a Representative Concentration Pathway climate scenario. The results indicated that the forest area increased in the northwest (11,691 km2) and the north (69 km2) of China but declined in the northeast (30,042 km2) over the past three decades. Moreover, the urban area has expanded by 1.3% in the TNR. Under the future climate condition, the hydrological regime will be influenced significantly by LCC. Those changes from 1986 to 2015 may alter the future hydrological cycle mainly by promoting runoff (3.24 mm/year) and decreasing evapotranspiration (3.23 mm/year) over the whole region. The spatial distribution of the effects may be extremely uneven: the effects in humid areas would be stronger than those in other areas. Besides, with rising temperatures and precipitation from 2020 to 2099, the LCC may heighten the risk of dryland expansion and flooding more than climate change alone. Despite uncertainties in the datasets and methods, the regional-scale hydrological model provides new insights into the extended impacts of ecological restoration and urbanization on the hydrological regime of the TNR.