bluuebunny/crossref_metadata_2025 huggingface dataset:
Multiple attention mechanisms-driven component fault location in optical networks with network-wide monitoring data
Authors: Zeng Chuidian , Zhang Jiawei , Wang Ruikun , Zhang Bojun , Ji Yuefeng
Published: January 2023

Fault location is an essential part of optical network operation and maintenance, and network operators have expectations to achieve highly accurate and precise fault location for reducing the cost of fault recovery. However, due to the scale of such networks, the volume of monitoring data (MD) is quite large, which poses a great challenge for fault location. An attention mechanism is an effective way to focus on the important information from massive input for the current task, which originates from the study of human vision. Targeting component fault location in optical networks, we propose an attention mechanism-based strategy, which consists of a sequence attention mechanism (SAT), a channel attention mechanism (CAT), a graph attention mechanism (GAT), and a fully connected neural network (FCNN). SAT, CAT, and GAT are applied for link, node, and network representation, respectively, taking corresponding MD as input. The FCNN is responsible for analyzing the correlation between MD and completing the fault location decision. All three attention mechanisms can filter out the more critical MD, assisting the FCNN to make more accurate decisions. We compare the performance of the proposed strategy and artificial neural networks (ANNs) in partial telemetry scenarios. Simulation results indicate that our strategy outperforms ANNs with respect to the accuracy of fault location by focusing on more critical MD and achieves a maximum improvement by 5.6%. Moreover, its feasibility with real data is verified on an experimental testbed consisting of hybrid optical-electrical switching nodes. Extensive results show that our strategy has the potential to achieve highly accurate fault location in real networks.
Ultrabright polarization-entangled photon pair source for frequency-multiplexed quantum communication in free-space
Authors: Brambila Emma , Gómez Rodrigo , Fazili Riza , Gräfe Markus , Steinlechner Fabian
Published: January 2023

The distribution of entanglement via satellite links will drastically extend the reach of quantum networks. Highly efficient entangled photon sources are an essential requirement towards overcoming high channel loss and achieving practical transmission rates in long-distance satellite downlinks. Here we report on an ultrabright entangled photon source that is optimized for long-distance free-space transmission. It operates in a wavelength range that is efficiently detected with space-ready single photon avalanche diodes (Si-SPADs), and readily provides pair emission rates that exceed the detector bandwidth (i.e., the temporal resolution). To overcome this limitation, we demultiplex the photon flux into wavelength channels that can be handled by current single photon detector technology. This is achieved efficiently by using the spectral correlations due to hyper-entanglement in polarization and frequency as an auxiliary resource. Combined with recent demonstrations of space-proof source prototypes, these results pave the way to a broadband long-distance entanglement distribution network based on satellites.
Inverse-designed polarization multiplexing non-uniformly distributed gratings for one-dimensional beam steering
Authors: Fu Tianyang , Yan Xin , Zhang Xia , Ren Xiaomin
Published: January 2023

Non-uniformly distributed gratings on the silicon-on-insulator platform for one-dimensional beam steering are designed by direct binary search inverse-design method. The gratings exhibit good emission directionality and far-field characteristics. Within a relatively small wavelength tuning range of 1517-1577 nm, the longitudinal scanning angle for TE and TM light is 23.65° and 10.81°, respectively, both of which are much larger than their uniform counterparts. By polarization multiplexing and etching depth optimization, a remarkable longitudinal scanning angle of 32.10° and high beam steering efficiency of 0.55°/nm are obtained. This work may pave the way for the development of miniaturized optical phased arrays with excellent beam steering performance.
Quantitative study of birefringence effects in fiber-based orthogonal-pump FWM systems
Authors: Liu Hao , Bottrill Kyle R. H. , Vitali Valerio , Taranta Austin , Petropoulos Periklis
Published: January 2023

Optical fibers have unwanted residual birefringence due to imperfections in fabrication processes and environmental conditions. This birefringence will randomize the state of polarization of propagating signals and may harm the performance of four-wave mixing based processing devices. Here, we present a quantitative study of the effects of birefringence in orthogonal-pump four-wave mixing systems, and identify different regions of operation of the optical fiber, mainly determined by the relative magnitude between the physical length L and beat length L
b
. This finding clarifies the characteristics of the complex interplay between birefringence and four-wave mixing and advises appropriate fiber length selection for minimized polarization dependent gain.
Analysis of the nonlinear propagation of incoherent pulses
Authors: Dorrer C.
Published: January 2023

The nonlinear propagation of incoherent optical pulses is studied using a normalized nonlinear Schrödinger equation and statistical analysis, demonstrating various regimes that depend on the field’s coherence time and intensity. The quantification of the resulting intensity statistics using probability density functions shows that, in the absence of spatial effects, nonlinear propagation leads to an increase in the likelihood of high intensities in a medium with negative dispersion, and a decrease in a medium with positive dispersion. In the latter regime, nonlinear spatial self-focusing originating from a spatial perturbation can be mitigated, depending on the coherence time and amplitude of the perturbation. These results are benchmarked against the Bespalov–Talanov analysis applied to strictly monochromatic pulses.
Simple route for high-throughput fabrication of metasurfaces using one-step UV-curable resin printing
Authors: Gong Jintao , Xiong Lingxing , Pu Mingbo , Guo Yinghui , Wen Yifeng , He Qiong , Li Xiong , Ma Xiaoliang , Luo Xiangang
Published: January 2023

Phase-gradient metasurfaces are two-dimensional (2D) optical elements that can manipulate light by imposing local, space-variant phase changes on an incident electromagnetic wave. These metasurfaces hold the potential and the promise to revolutionize photonics by providing ultrathin alternatives for a wide range of common optical elements such as bulky refractive optics, waveplates, polarizers, and axicons. However, the fabrication of state-of-the-art metasurfaces typically requires some time-consuming, expensive, and possibly hazardous processing steps. To overcome these limitations on conventional metasurface fabrication, a facile methodology to produce phase-gradient metasurfaces through one-step UV-curable resin printing is developed by our research group. The method dramatically reduces the required processing time and cost, as well as eliminates safety hazards. As a proof-of-concept, the advantages of the method are clearly demonstrated via a rapid reproduction of high-performance metalenses based on the Pancharatnam-Berry phase gradient concept in the visible spectrum.
Low-modal-crosstalk orthogonal combine reception for degenerate modes in IM/DD MDM transmission
Authors: Cui Jian , Gao Yuyang , Huang Shuailuo , Yu Jinyi , Shen Lei , Zhang Lei , Yan Changkun , Yang Liubo , Wang Ruichun , He Yongqi , Chen Zhangyuan , Li Juhao
Published: January 2023

Weakly-coupled mode division multiplexing (MDM) techniques supporting intensity modulation and direct detection (IM/DD) transmission is a promising candidate to enhance the capacity of short-reach applications such as optical interconnections, in which low-modal-crosstalk mode multiplexers/demultiplexers (MMUX/MDEMUX) are highly desired. In this paper, we firstly propose an all-fiber low-modal-crosstalk orthogonal combine reception scheme for degenerate linearly-polarized (LP) modes, in which signals in both degenerate modes are firstly demultiplexed into the LP01 mode of single-mode fibers, and then are multiplexed into mutually orthogonal LP01 and LP11 modes of a two-mode fiber for simultaneous detection. Then a pair of 4-LP-mode MMUX/MDEMUX consisting of cascaded mode-selective couplers and orthogonal combiners are fabricated with side-polishing processing, which achieve low back-to-back modal crosstalk of lower than -18.51 dB and insertion loss of lower than 3.81 dB for all the 4 modes. Finally, a stable real-time 4 modes × 4λ × 10 Gb/s MDM-wavelength division multiplexing (WDM) transmission over 20-km few-mode fiber is experimentally demonstrated. The proposed scheme is scalable to support more modes and can pave the way to practical implementation of IM/DD MDM transmission applications.
Bound states in the continuum in asymmetric one-dimensional photonic crystal systems guided by anisotropy
Authors: Liu Ziyi , Li Xiangning , Chen Chen , Wang Xu , Gao Wenya , Ye Weimin , Li Li , Liu Jianlong
Published: January 2023

Bound states in the continuum (BICs) have been widely observed in many symmetric geometries in the optical system during the last decade. Here, we consider a scenario in which the structure is designed asymmetrically with anisotropic birefringent material embedded in one-dimensional photonic crystals. This kind of new shape opens the possibility of obtaining symmetry-protected BICs (SP-BICs) and Friedrich–Wintgen BICs (FW-BICs) form in tunable anisotropy axis tilt. Interestingly, these BICs can be observed as high-Q resonances by variation of the system’s parameters, such as the incident angle, which means the structure without being injected at Brewster's angle can also achieve BICs. Our findings might achieve active regulation and are easy to manufacture.
Interoceptive differences in elite sprint and long-distance runners: A multidimensional investigation
Authors: Seabury Tom , Benton David , Young Hayley A.
Published: January 2023

Interoception, the process of detecting and interpreting bodily sensations, may facilitate self-regulation and thereby play a crucial role in achieving elite performance in competitive sports. However, there is a lack of research conducted in world-class athletes. In the present research, two studies examined self-reported (interoceptive sensibility) and behavioural (interoceptive accuracy) interoception in elite (top 100 ranking) sprint and long-distance runners, and non-athletes. Study 1 used the Multidimensional Assessment of Interoceptive Awareness Questionnaire. Sprinters reported having better regulation of attention to internal sensations, greater emotional awareness, better self-regulation, and reported a greater propensity to listen to their body for insight, than distance runners. Compared to non-athletes, sprinters and distance runners had more bodily trust, attention regulation, and self-regualtion. Additionally, elite athletes reported lower emotional awareness, self-regulation, and body listening. Study 2 examined cardioception using two tasks: The Heartbeat Counting Task, and The Heartbeat Detection Task. Elite and non-elite runners performed the tasks under two conditions; in silence, and whilst listening to pre-recorded crowd noise that simulated the live sounds of spectators during a sporting event. Sprinters and distance runners were able to maintain heartbeat detection accuracy when distracted, whereas non-athletes could not. Across both tasks, compared to non-athletes, sprinters and distance runners were more confident than non-athletes in their interoceptive percept. Additionally, elite athletes compared to non-elite athletes were less accurate when counting their heartbeat and were characterised by a higher interoceptive prediction error. Athletic populations have altered interoceptive abilities.
Offline events and online hate
Authors: Lupu Yonatan , Sear Richard , Velásquez Nicolas , Leahy Rhys , Restrepo Nicholas Johnson , Goldberg Beth , Johnson Neil F.
Published: January 2023

Online hate speech is a critical and worsening problem, with extremists using social media platforms to radicalize recruits and coordinate offline violent events. While much progress has been made in analyzing online hate speech, no study to date has classified multiple types of hate speech across both mainstream and fringe platforms. We conduct a supervised machine learning analysis of 7 types of online hate speech on 6 interconnected online platforms. We find that offline trigger events, such as protests and elections, are often followed by increases in types of online hate speech that bear seemingly little connection to the underlying event. This occurs on both mainstream and fringe platforms, despite moderation efforts, raising new research questions about the relationship between offline events and online speech, as well as implications for online content moderation.
Live births from urine derived cells
Authors: Olsson P. Olof , Yeonwoo Jeong , Park Kyumi , Yoo Yeong-Min , Hwang W. S.
Published: January 2023

Here we report urine-derived cell (UDC) culture and subsequent use for cloning which resulted in the successful development of cloned canine pups, which have remained healthy into adulthood. Bovine UDCs were used in vitro to establish comparative differences between cell sources. UDCs were chosen as a readily available and noninvasive source for obtaining cells. We analyzed the viability of cells stored in urine over time and could consistently culture cells which had remained in urine for 48hrs. Cells were shown to be viable and capable of being transfected with plasmids. Although primarily of epithelial origin, cells were found from multiple lineages, indicating that they enter the urine from more than one source. Held in urine, at 4°C, the majority of cells maintained their membrane integrity for several days. When compared to in vitro fertilization (IVF) derived embryos or those from traditional SCNT, UDC derived embryos did not differ in total cell number or in the number of DNA breaks, measured by TUNEL stain. These results indicate that viable cells can be obtained from multiple species’ urine, capable of being used to produce live offspring at a comparable rate to other cell sources, evidenced by a 25% pregnancy rate and 2 live births with no losses in the canine UDC cloning trial. This represents a noninvasive means to recover the breeding capacity of genetically important or infertile animals. Obtaining cells in this way may provide source material for human and animal studies where cells are utilized.
Institutional courage buffers against institutional betrayal, protects employee health, and fosters organizational commitment following workplace sexual harassment
Authors: Smidt Alec M. , Adams-Clark Alexis A. , Freyd Jennifer J.
Published: January 2023

Workplace sexual harassment is associated with negative psychological and physical outcomes. Recent research suggests that harmful institutional responses to reports of wrongdoing–called institutional betrayal—are associated with additional psychological and physical harm. It has been theorized that supportive responses and an institutional climate characterized by transparency and proactiveness—called institutional courage—may buffer against these negative effects. The current study examined the association of institutional betrayal and institutional courage with workplace outcomes and psychological and physical health among employees reporting exposure to workplace sexual harassment. Adults who were employed full-time for at least six months were recruited through Amazon’s Mechanical Turk platform and completed an online survey (N = 805). Of the full sample, 317 participants reported experiences with workplace sexual harassment, and only this subset of participants were included in analyses. We used existing survey instruments and developed the Institutional Courage Questionnaire-Specific to assess individual experiences of institutional courage within the context of workplace sexual harassment. Of participants who experienced workplace sexual harassment, nearly 55% also experienced institutional betrayal, and 76% experienced institutional courage. Results of correlational analyses indicated that institutional betrayal was associated with decreased job satisfaction, organizational commitment, and increased somatic symptoms. Institutional courage was associated with the reverse. Furthermore, results of multiple regression analyses indicated that institutional courage appeared to attenuate negative outcomes. Overall, our results suggest that institutional courage is important in the context of workplace sexual harassment. These results are in line with previous research on institutional betrayal, may inform policies and procedures related to workplace sexual harassment, and provide a starting point for research on institutional courage.
Relating parental stress with sleep disorders in parents and children
Authors: Merrill Ray M. , Slavik Kayla R.
Published: January 2023

Objective

To assess whether child sleep disorders positively correlate with parental insomnia, hypersomnia, and sleep apnea, and whether parental and child sleep disorders simultaneously positively associate with parental stress. Potential modifying influences of these associations by age, sex, and marital status will be considered.

Methods

Analyses are based on 14,009 employees aged 18–64 with dependent children (n = 44,157) insured by Deseret Mutual Benefit Administrator (DMBA) in 2020. Rate ratios are adjusted for age, sex, and marital status.

Results

The rate of parental stress is 3.00 (95% CI 2.33–4.85) times greater for those with insomnia and 1.88 (95% CI 1.59–2.22) times greater for those with sleep apnea. There is no increased risk of stress for those with hypersomnia. The number of dependent children filing one or more medical claims for a sleep disorder is 2.0%. Mean age is significantly older among those with a sleep disorder (17.1 vs. 14.4, t p < .0001). Child sex is not associated with the risk of having a sleep disorder. The rate of employee insomnia is 111% greater if their child has a sleep disorder, and employee sleep apnea is 115% greater if their child has a sleep disorder. The association between child sleep disorders and sleep apnea decreases with employee age (Wald chi-square p = 0.0410). The rate of employee stress is 90% greater if their child has a sleep disorder, 189% greater if they have insomnia, and 81% greater if they have sleep apnea. The strength of the association between insomnia and stress is greater for women (Wald Chi-square p = 0.0114), between sleep apnea and stress is greater for women (Wald chi-square p = 0.0010), and between sleep apnea and stress is greater for singles (Wald chi-square p = 0.0010).

Conclusions

Better understanding the connection between parent and child sleep problems and parent stress, and modifying influences, may improve treatment of these disorders.
Associations of dietary patterns with obesity and weight change for adults aged 18–65 years: Evidence from the China Health and Nutrition Survey (CHNS)
Authors: Chen Yang , Kang Minji , Kim Hyojin , Xu Wanghong , Lee Jung Eun
Published: January 2023

Background

Obesity has become a significant public health problem within China. By observing dietary patterns, an individual’s actual dietary habit of nutrients or foods in combination can be identified. We aimed to examine dietary patterns in relation to the risk of obesity (body mass index (BMI) ≥ 25 kg/m2) and weight change (average weight change per five years) within a Chinese cohort.

Methods

We analyzed the data from 6,677 adults aged 18–65 years in the China Health and Nutrition Survey 1997–2015. Westernized, traditional Chinese, and high-starch plant-based dietary patterns were identified by principal components analysis. We estimated relative risks (RRs) and least-squares means (LS-means) with 95% confidence intervals (CIs) using the Cox proportional hazards models and the generalized linear models, respectively.

Results

High adherence to the Westernized dietary pattern was associated with increased risks of obesity and weight gain. Comparing top with bottom quintiles, RR (95% CI) for obesity (BMI ≥ 25 kg/m2) was 1.57 (1.26–1.95; P for trend < 0.001). LS-means of weight change (kg/5 years) were 1.73 (0.98–2.47) and 1.13 (0.39–1.87; P for trend = 0.036) in the top and bottom quintiles, respectively. Increased weight gain among those following the Westernized dietary pattern was stronger in the Southern region than the Northern region. There was a slight hint of an inverse association between the traditional Chinese dietary pattern and obesity. We did not find any significant association for the high-starch plant-based dietary pattern.

Conclusions

The Westernized dietary pattern increased the risk of obesity among Chinese adults. Weight gain associated with Western dietary pattern was more pronounced in the Southern region than in the Northern region. Our study will provide helpful data in developing dietary guidelines for the prevention of obesity specific to different regions in China.
Use of complete medication history to identify and correct transitions-of-care medication errors at psychiatric hospital admission
Authors: Vargas Victoria , Blakeslee Weston W. , Banas Colin A. , Teter Christian , Dupuis-Dobson Katherine , Aboud Carol
Published: January 2023

Methods for categorizing the scale and severity of medication errors corrected by pharmacy staff during admission medication reconciliation using complete medication history continue to evolve. We established a rating scale that is effective for generating error reports to health system quality leadership. These reports are needed to quantify the value of investment in transitions-of-care pharmacy staff. All medication errors that were reported by pharmacy staff in the admission medication reconciliation process during a period of 6 months were eligible for inclusion. Complete medication history data source was utilized by admitting providers and all pharmacist staff and a novel medication error scoring methodology was developed. This methodology included: medication error category, medication error type, potential medication error severity, and medication non-adherence. We determined that 82 medication errors were detected from 72 patients and assessed that 74 of these errors may have harmed patients if they were not corrected through pharmacist intervention. Most of these errors were dosage discrepancies and omissions. With hospital system budgets continually becoming leaner, it is important to measure the effectiveness and value of staff resources to optimize patient care. Pharmacists performing admission medication reconciliation can detect subtle medication discrepancies that may be overlooked by other clinician types. This methodology can serve as a foundation for error reporting and predicting the severity of adverse drug events.
The changing meaning of ‘home’ in the work of South African women academics during the pandemic-enforced lockdown
Authors: Walters Cyrill , Ronnie Linda , Jansen Jonathan , Kriger Samantha
Published: January 2023

This article shows how the meaning of home and ‘working from home’ were fundamentally transformed by the pandemic-enforced lockdown for women academics. Drawing on the experiences of more than 2,000 women academics, we show how the enduring concept of home as a place of refuge from the outside world was replaced with a new and still unsettled notion of home as a gendered space that is a congested, competitive, and constrained setting for women’s academic work. In this emerging new place for living and working, home becomes a space that is claimed, conceded, and constantly negotiated between women academics and their partners as well as the children and other occupants under the same roof. Now, as before, home remains a deeply unequal place for women’s work, with dire consequences for academic careers. It is therefore incumbent upon women academics and higher education institutions to develop a deep understanding of the social meanings of home for academics, and the implications for the ‘new normal’ of working from home.
The ecological discourse analysis of news discourse based on deep learning from the perspective of ecological philosophy
Authors: Zhang Biyun , Chandran Sandaran Shanti , Feng Jing
Published: January 2023

Recently, ecological damage and environmental pollution have become increasingly serious. Experts in various fields have started to study related issues from diverse points of view. To prevent the accelerated deterioration of the ecological environment, ecolinguistics emerged. Eco-critical discourse analysis is one of the important parts of ecolinguistics research, that is, it is a critical discourse analysis of the use of language from the perspective of the language’s ecological environment. Firstly, an ecological tone and modality system are constructed from an ecological perspective. Under the guidance of the ecological philosophy of "equality, harmony, and symbiosis", this study conducts an ecological discourse analysis on the Sino-US trade friction reports, aiming to present the similarities and differences between the two newspapers’ trade friction discourses and to reveal the ecological significance of international ecological factors in the discourse. Secondly, this method establishes a vector expression of abstract words based on emotion dictionary resources and introduces emotion polarity and part-of-speech features of words. Then the word vector is formed into the text feature matrix, which is used as the input of the Convolutional Neural Network (CNN) model, and the Back Propagation algorithm is adopted to train the model. Finally, in the light of the trained CNN model, the unlabeled news is predicted, and the experimental results are analyzed. The results reveal that during the training process of Chinese and English datasets, the accuracy of the training set can reach nearly 100%, and the loss rate can be reduced to 0. On the test set, the classification accuracy of Chinese text can reach 83%, while that of English text can reach 90%, and the experimental results are ideal. This study provides an explanatory approach for ecological discourse analysis on the news reports of Sino-US trade frictions and has certain guiding significance for the comparative research on political news reports under different ideologies between China and the United States.
Genome concentration, characterization, and integrity analysis of recombinant adeno-associated viral vectors using droplet digital PCR
Authors: Prantner Andrew , Maar Dianna
Published: January 2023

Precise, reproducible characterization of AAV is critical for comparing preclinical results between laboratories and determining a safe and effective clinical dose for gene therapy applications. In this study, we systematically evaluated numerous parameters to produce a simple and robust ddPCR protocol for AAV characterization. The protocol uses a low ionic strength buffer containing Pluronic-F68 and polyadenylic acid to dilute the AAV into the ddPCR concentration range and a 10-minute thermal capsid lysis prior to assembling ddPCR reactions containing MspI. A critical finding is that the buffer composition affected the ITR concentration of AAV but not the ITR concentration of a double stranded plasmid, which has implications when using a theoretical, stoichiometric conversion factor to obtain the titer based on the ITR concentration. Using this protocol, a more comprehensive analysis of an AAV vector formulation was demonstrated with multiple ddPCR assays distributed throughout the AAV vector genome. These assays amplify the ITR, regulatory elements, and eGFP transgene to provide a more confident estimate of the vector genome concentration and a high-resolution characterization of the vector genome identity. Additionally, we compared two methods of genome integrity analysis for three control sample types at eight different concentrations for each sample. The genome integrity was independent of sample concentration and the expected values were obtained when integrity was determined based on the excess number of positive droplets relative to the number of double positive droplets expected by chance co-encapsulation of two DNA targets. The genome integrity was highly variable and produced unexpected values when the double positive droplet percentage was used to calculate the genome integrity. A protocol using a one-minute thermal capsid lysis prior to assembling ddPCR reactions lacking a restriction enzyme used the non-ITR assays in a duplex ddPCR milepost experiment to determine the genome integrity using linkage analysis.
The educational effects of emergency remote teaching practices—The case of covid-19 school closure in Italy
Authors: Bertoletti Alice , Soncin Mara , Cannistrà Marta , Agasisti Tommaso
Published: January 2023

The disruption caused by the Covid-19 pandemic generated an unprecedented situation, in which digital learning, in the form of Emergency Remote Teaching, was the only possible form of schooling. Italy soon decided to close schools as a measure to counter the spread of the virus. Although the Ministry of Education suggested general guidelines, school principals and teachers were autonomous in deciding how to best organise their educational activities. The first objective of our study is to identify subgroups of teachers, based on the use of digital tools during the Covid-19 emergency. Secondly, we explore how subgroups differ in terms of teachers’ satisfaction and students’ performance. To this end, we integrate information from an ad hoc survey completed by 1,407 primary and lower secondary teachers in Italy, with the students’ standardised test scores provided by INVALSI. Data have been analysed through a 3-step latent class analysis. The findings reveal that one third of the teachers showed resistance to the use of digital technologies and focused mainly on asynchronous teaching. Teachers that used a broader set of digital instruments, instead, were more satisfied with their teaching practices. A more articulated use of technology for teaching activities was also positively associated with higher students’ performance in 2021.
Tumor heterogeneity and carcinoma in resected specimens of gastric low-grade dysplasia: A retrospective single center study
Authors: Shin Ga-Yeong , Park Jun Young , Lee Sung Hak , Cho Yu Kyung , Choi Myung-Gyu , Park Jae Myung
Published: January 2023

Lesions diagnosed as gastric low-grade dysplasia (LGD) may be pathologically upgraded to early gastric cancer (EGC) or high-grade dysplasia (HGD) after endoscopic resection (ER). In this study, we investigated the risk factors for pathological upgrades after ER and assessed the reason for these upgrades by retrospectively analyzing ER data between January 1999 and December 2019. We enrolled patients with LGD confirmed by forceps biopsy; the patients were classified into pathologically concordant (LGD) and upgraded (HGD and EGC) groups according to the pathology of their resected specimen. To determine the risk factors for upgrade, we compared the endoscopic findings of the concordant and upgraded groups via 1:1 matched case-control design. To find the reasons for discordance, all upgraded cases were pathologically re-evaluated. Among 1,643 cases of LGD, pathological upgrades were observed in 423 (25.7%) resected specimens and EGC was found in 111 (6.7%) lesions. After matching the upgraded and concordant cases, lesion sizes exceeding 1.5 cm (odds ratio (OR): 1.8; 95% CI: 1.1–3.0), mucosal nodularity (OR: 10.8; 95% CI: 5.6–21.0), heterogeneous color (OR: 3.0; 95% CI: 1.7–5.3), presence of erosion (OR: 2.7; 95% CI: 1.8–5.3), and open-type gastric atrophy (OR: 2.9; 95% CI: 1.7–4.9) were noted to be significantly associated with upgraded pathology to EGC. Among the EGC cases, 99 (89.2%) were found to have pre-existing dysplasia. In conclusion, endoscopic evaluations should be performed because of possible pathological upgrades and co-existence of carcinomas in LGDs, especially when they exhibit surface nodularity, erosion, heterogeneous color, and large size.
Targeted next-generation sequencing has incremental value in the diagnostic work-up of patients with suspect pancreatic masses; a multi-center prospective cross sectional study
Authors: Achterberg Friso B. , Mulder Babs G. Sibinga , Janssen Quisette P. , Koerkamp Bas Groot , Hol Lieke , Quispel Rutger , Bonsing Bert A. , Vahrmeijer Alexander L. , van Eijck Casper H. J. , Roos Daphne , Perk Lars E. , van der Harst Erwin , Coene Peter-Paul L. O. , Doukas Michail , Smedts Frank M. M. , Kliffen Mike , van Velthuysen Marie-Louise F. , Terpstra Valeska , Sarasqueta Arantza Farina , Morreau Hans , Mieog J. Sven D.
Published: January 2023

Background

The diagnostic process of patients with suspect pancreatic lesions is often lengthy and prone to repeated diagnostic procedures due to inconclusive results. Targeted Next-Generation Sequencing (NGS) performed on cytological material obtained with fine needle aspiration (FNA) or biliary duct brushing can speed up this process. Here, we study the incremental value of NGS for establishing the correct diagnosis, and subsequent treatment plan in patients with inconclusive diagnosis after regular diagnostic work-up for suspect pancreatic lesions.

Methods

In this prospective cross-sectional cohort study, patients were screened for inclusion in four hospitals. NGS was performed with AmpliSeq Cancer Hotspot Panel v2 and v4b in patients with inconclusive cytology results or with an uncertain diagnosis. Diagnostic results were evaluated by the oncology pancreatic multidisciplinary team. The added value of NGS was determined by comparing diagnosis (malignancy, cystic lesion or benign condition) and proposed treatment plan (exploration/resection, neoadjuvant chemotherapy, follow-up, palliation or repeated FNA) before and after integration of NGS results. Final histopathological analysis or a 6-month follow-up period were used as the reference standard in case of surgical intervention or non-invasive treatment, respectively.

Results

In 50 of the 53 included patients, cytology material was sufficient for NGS analysis. Diagnosis before and after integration of NGS results differed in 24% of the patients. The treatment plan was changed in 32% and the diagnosis was substantiated by the NGS data in 44%. Repetition of FNA/brushing was prevented in 14% of patients. All changes in treatment plan were correctly made after integration of NGS. Integration of NGS increased overall diagnostic accuracy from 68% to 94%.

Interpretation

This study demonstrates the incremental diagnostic value of NGS in patients with an initial inconclusive diagnosis. Integration of NGS results can prevent repeated EUS/FNA, and can also rigorously change the final diagnosis and treatment plan.
Characterisation of human milk bacterial DNA profiles in a small cohort of Australian women in relation to infant and maternal factors
Authors: Sindi Azhar S. , Cheema Ali S. , Trevenen Michelle L. , Geddes Donna T. , Payne Matthew S. , Stinson Lisa F.
Published: January 2023

Human milk is composed of complex microbial and non-microbial components that shape the infant gut microbiome. Although several maternal and infant factors have been associated with human milk microbiota, no study has investigated this in an Australian population. Therefore, we aimed to investigate associations between human milk bacterial composition of Australian women and maternal factors (body mass index (BMI), mode of delivery, breast pump use, allergy, parity) and infant factors (sex, mode of feeding, pacifier use, and introduction of solids). Full-length 16S rRNA gene sequencing was used to characterise milk bacterial DNA profiles. Milk from mothers with a normal BMI had a higher relative abundance ofStreptococcus australisthan that of underweight mothers, while milk from overweight mothers had a higher relative abundance ofStreptococcus salivariuscompared with underweight and obese mothers. Mothers who delivered vaginally had a higher relative abundance ofStreptococcus mitisin their milk compared to those who delivered via emergency caesarean section. Milk of mothers who used a breast pump had a higher relative abundance ofStaphylococcus epidermidisandStreptococcus parasanguinis. Milk of mothers whose infants used a pacifier had a higher relative abundance ofS.australisandStreptococcus gwangjuense. Maternal BMI, mode of delivery, breast pump use, and infant pacifier use are associated with the bacterial composition of human milk in an Australian cohort. The data from this pilot study suggests that both mother and infant can contribute to the human milk microbiome.
TYMV and TRV infect Arabidopsis thaliana by expressing weak suppressors of RNA silencing and inducing host RNASE THREE LIKE1
Authors: Sehki Hayat , Yu Agnès , Elmayan Taline , Vaucheret Hervé
Published: January 2023

Post-Transcriptional Gene Silencing (PTGS) is a defense mechanism that targets invading nucleic acids of endogenous (transposons) or exogenous (pathogens, transgenes) origins. During plant infection by viruses, virus-derived primary siRNAs target viral RNAs, resulting in both destruction of single-stranded viral RNAs (execution step) and production of secondary siRNAs (amplification step), which maximizes the plant defense. As a counter-defense, viruses express proteins referred to as Viral Suppressor of RNA silencing (VSR). Some viruses express VSRs that totally inhibit PTGS, whereas other viruses express VSRs that have limited effect. Here we show that infection with the Turnip yellow mosaic virus (TYMV) is enhanced in Arabidopsis ago1, ago2 and dcl4 mutants, which are impaired in the execution of PTGS, but not in dcl2, rdr1 and rdr6 mutants, which are impaired in the amplification of PTGS. Consistently, we show that the TYMV VSR P69 localizes in siRNA-bodies, which are the site of production of secondary siRNAs, and limits PTGS amplification. Moreover, TYMV induces the production of the host enzyme RNASE THREE-LIKE 1 (RTL1) to further reduce siRNA accumulation. Infection with the Tobacco rattle virus (TRV), which also encodes a VSR limiting PTGS amplification, induces RTL1 as well to reduce siRNA accumulation and promote infection. Together, these results suggest that RTL1 could be considered as a host susceptibility gene that is induced by viruses as a strategy to further limit the plant PTGS defense when VSRs are insufficient.
Characterization of a new CCCTC-binding factor binding site as a dual regulator of Epstein-Barr virus latent infection
Authors: Lee Sun Hee , Kim Kyoung-Dong , Cho Miyeon , Huh Sora , An Seong Ho , Seo Donghyun , Kang Kyuhyun , Lee Minhee , Tanizawa Hideki , Jung Inuk , Cho Hyosun , Kang Hyojeung
Published: January 2023

Distinct viral gene expression characterizes Epstein-Barr virus (EBV) infection in EBV-producing marmoset B-cell (B95-8) and EBV-associated gastric carcinoma (SNU719) cell lines. CCCTC-binding factor (CTCF) is a structural chromatin factor that coordinates chromatin interactions in the EBV genome. Chromatin immunoprecipitation followed by sequencing against CTCF revealed 16 CTCF binding sites in the B95-8 and SNU719 EBV genomes. The biological function of one CTCF binding site (S13 locus) located on the BamHI A right transcript (BART) miRNA promoter was elucidated experimentally. Microscale thermophoresis assay showed that CTCF binds more readily to the stable form than the mutant form of the S13 locus. EBV BART miRNA clusters encode 22 miRNAs, whose roles are implicated in EBV-related cancer pathogenesis. The B95-8 EBV genome lacks a 11.8-kb EcoRI C fragment, whereas the SNU719 EBV genome is full-length. ChIP-PCR assay revealed that CTCF, RNA polymerase II, H3K4me3 histone, and H3K9me3 histone were more enriched at S13 and S16 (167-kb) loci in B95-8 than in the SNU719 EBV genome. 4C-Seq and 3C-PCR assays using B95-8 and SNU719 cells showed that the S13 locus was associated with overall EBV genomic loci including 3-kb and 167-kb region in both EBV genomes. We generated mutations in the S13 locus in bacmids with or without the 11.8-kb BART transcript unit (BART(+/-)). The S13 mutation upregulated BART miRNA expression, weakened EBV latency, and reduced EBV infectivity in the presence of EcoRI C fragment. Another 3C-PCR assay using four types of BART(+/-)·S13(wild-type(Wt)/mutant(Mt)) HEK293-EBV cells revealed that the S13 mutation decreased DNA associations between the 167-kb region and 3-kb in the EBV genome. Based on these results, CTCF bound to the S13 locus along with the 11.8-kb EcoRI C fragment is suggested to form an EBV 3-dimensional DNA loop for coordinated EBV BART miRNA expression and infectivity.
Reinforcement amid genetic diversity in the Candida albicans biofilm regulatory network
Authors: Cravener Max V. , Do Eunsoo , May Gemma , Zarnowski Robert , Andes David R. , McManus C. Joel , Mitchell Aaron P.
Published: January 2023

Biofilms of the fungal pathogen Candida albicans include abundant long filaments called hyphae. These cells express hypha-associated genes, which specify diverse virulence functions including surface adhesins that ensure biofilm integrity. Biofilm formation, virulence, and hypha-associated gene expression all depend upon the transcription factor Efg1. This transcription factor has been characterized extensively in the C. albicans type strain SC5314 and derivatives, but only recently has its function been explored in other clinical isolates. Here we define a principal set of Efg1-responsive genes whose expression is significantly altered by an efg1Δ/Δ mutation across 17 clinical isolates. This principal gene set includes 68 direct Efg1 targets, whose 5’ regions are bound by Efg1 in five clinical isolates, and 42 indirect Efg1 targets, whose 5’ regions are not detectably bound by Efg1. Three direct Efg1 target genes encode transcription factors—BRG1, UME6, and WOR3 –whose increased expression in an efg1Δ/Δ mutant restores expression of multiple indirect and direct principal targets, as well as biofilm formation ability. Although BRG1 and UME6 are well known positive regulators of hypha-associated genes and biofilm formation, WOR3 is best known as an antagonist of Efg1 in the sexual mating pathway. We confirm the positive role of WOR3 in biofilm formation with the finding that a wor3Δ/Δ mutation impairs biofilm formation in vitro and in an in vivo biofilm model. Positive control of Efg1 direct target genes by other Efg1 direct target genes–BRG1, UME6, and WOR3 –may buffer principal Efg1-responsive gene expression against the impact of genetic variation in the C. albicans species.
Short tandem repeat polymorphism in the promoter region of cyclophilin 19B drives its transcriptional upregulation and contributes to drug resistance in the malaria parasite Plasmodium falciparum
Authors: Kucharski Michal , Wirjanata Grennady , Nayak Sourav , Boentoro Josephine , Dziekan Jerzy Michal , Assisi Christina , van der Pluijm Rob W. , Miotto Olivo , Mok Sachel , Dondorp Arjen M. , Bozdech Zbynek
Published: January 2023

Resistance of the human malaria parasites, Plasmodium falciparum, to artemisinins is now fully established in Southeast Asia and is gradually emerging in Sub-Saharan Africa. Although nonsynonymous SNPs in the pfk13 Kelch-repeat propeller (KREP) domain are clearly associated with artemisinin resistance, their functional relevance requires cooperation with other genetic factors/alterations of the P. falciparum genome, collectively referred to as genetic background. Here we provide experimental evidence that P. falciparum cyclophilin 19B (PfCYP19B) may represent one putative factor in this genetic background, contributing to artemisinin resistance via its increased expression. We show that overexpression of PfCYP19B in vitro drives limited but significant resistance to not only artemisinin but also piperaquine, an important partner drug in artemisinin-based combination therapies. We showed that PfCYP19B acts as a negative regulator of the integrated stress response (ISR) pathway by modulating levels of phosphorylated eIF2α (eIF2α-P). Curiously, artemisinin and piperaquine affect eIF2α-P in an inverse direction that in both cases can be modulated by PfCYP19B towards resistance. Here we also provide evidence that the upregulation of PfCYP19B in the drug-resistant parasites appears to be maintained by a short tandem repeat (SRT) sequence polymorphism in the gene’s promoter region. These results support a model that artemisinin (and other drugs) resistance mechanisms are complex genetic traits being contributed to by altered expression of multiple genes driven by genetic polymorphism at their promoter regions.
Gaga and naming as eco-somatic practices of enchantment
Authors: Sanger Andrew
Published: January 2023

This article will trace theories of enchantment and disenchantment in the social sciences to craft a theoretical framework of enchantment as a form of ecological relationality. The framework will be supported by examples drawn from fieldwork completed between 2017 and 2021 in the United Kingdom and Israel of the contemporary somatic practice Gaga, a workshop on blessing and cursing facilitated by Claire MacDonald, and a week-long workshop with Sandra Reeve. These examples, expanded by sections of ethnographic reflection denoted in italics, demonstrate how a contemporary theory of enchantment, through somatic inquiry, may re-instil agency in the cold, mechanized world of modernity and open up channels of possibility to reimagine kinship.
From museum aesthetics to everyday aesthetics: Narratives about Saigon in The Lover by Jean-Jacques Annaud and Bar Girls by Le Hoang
Authors: Nguyen Thi Nhu Trang
Published: January 2023

Saigon is an urban area that has undergone many political and historical upheavals. This study focuses on aesthetic qualities in an examination of The Lover by Jean-Jacques Annaud and Bar Girls by Le Hoang, which together contrast the image of Saigon during two different periods. I argue that Annaud presents the image of a colonial Saigon from a perspective grounded in nostalgia and memories, utilizing the techniques of museum aesthetics to juxtapose western and eastern spaces. Meanwhile, Le Hoang highlights the contemporary city of Saigon, reflecting in his film the qualities of an everyday aesthetics.
When to Stop Surveillance: Pancreatic Cysts
Authors: Petrov Maxim S.
Published: January 2023

Surveillance of mucinous pancreatic cysts is a key to reducing pancreatic cancer risk and detecting malignancy early. However, while the management of cysts with high-risk and worrisome features is fairly straightforward, surveillance of patients with low-risk branch-duct intraductal papillary mucinous neoplasms has long presented gastroenterologists with the challenging question of discontinuation of surveillance. Up-to-date evidence supports the cessation of follow-up in these patients depending on both interval stability of the cyst and cyst size. Based on these criteria, discontinuation of surveillance at either 5 years or 10 years is recommended. Oversurveillance of patients with pancreatic cysts in the absence of high-risk and worrisome features is discouraged.