bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessing the unassessed marine recreational fishery in the Eastern Cantabrian coast
Authors: Bachiller Eneko , Korta Maria , Mateo Maria , Mugerza Estanis , Zarauz Lucia
Published: August 2022

Within the first attempt to assess marine recreational fisheries (MRF) along the coastal villages of the Basque Country (Eastern Cantabrian Sea), off-site surveys were carried out from 2015 to 2019 to estimate captures of the main targeted species by shore fishing, boat fishing and spearfishing. Phone calls got better response rates (>50%) than email questionnaires (<25%). Spearfishing population was smaller (1000 licenses, <2% of total MRF effort) and younger, whereas boat fishers (5000 licenses, 9% MRF effort) were generally older and more experienced. Shore fishing was the most extended MRF activity (50000 licenses, 90% of MRF effort), showing wider age and experience ranges. Boat fishing targeting albacore in summer was the main MRF activity interacting with regional commercial small-scale fishery. Squids were also important for both shore and boat fishing, followed by seabass, the main target species for shore anglers. Despite surveys were primarily designed to gather information about these three species, they also evidenced, moreover with clear underestimation bias, frequent captures of generally unassessed species, such as sargo-bream (Diplodus spp.), gilthead seabream (Sparus aurata), common dentex (Dentex dentex), red mullet (Mullus surmuletus) or scorpionfish (Scorpaena spp.). Fishers with more experience, as well as spearfishers in general, captured a wider diversity of species, but individual fishers were more specialized (i.e., showing less variation between trips). Our off-site survey assessment highlights the importance of incorporating multispecies sampling schemes to develop future MRF assessment criteria, within a context of an ecosystem approach that should also consider potential interactions with commercial small-scale fishery.
Superior gait performance and balance ability in Latin dancers
Authors: Liu Yen-Ting , Lin Ang-Chieh , Chen Szu-Fu , Shih Chih-Jen , Kuo Tien-Yun , Wang Fu-Cheng , Lee Pei-Hsin , Lee Adeline Peiling
Published: August 2022

Background

Latin dance consists of various fast and stability-challenging movements that require constant body adjustments to maintain proper posture and balance. Although human gaits are assumed to be symmetrical, several factors can contribute to asymmetrical behavior of the lower extremities in healthy adults. These include lower limb dominance, ground reaction forces, lower limb muscle power, foot placement angle, and range of joint motion. Gait impairment can lead to a high risk of falling, diminished mobility, and even cognition impairment. We hypothesized that Latin dancers might have a more symmetric gait pattern and better balance ability than healthy non-dancer controls.

Methods

We investigated the impact of Latin dance training on gait behaviors and body balance. We recruited twenty Latin dancers and 22 normal healthy subjects to conduct walking experiments and one-leg stance tests, and we measured their kinematic data by inertial measurement units. We then defined four performance indexes to assess gait performance and body stability to quantify the potential advantages of dance training.

Results

We found that the two gait asymmetric indexes during the walking test and the two performance indexes during the one-leg stance tests were better in Latin dancers compared with the healthy control group. The results confirmed the superiority of Latin dancers over the healthy control group in gait symmetry and balance stability. Our results suggest that Latin dancing training could effectively strengthen lower limb muscles and core muscle groups, thereby improving coordination and enhancing gait performance and balance.

Conclusion

Latin dance training can benefit gait performance and body balance. Further studies are needed to investigate the effect of Latin dance training on gait and balance outcomes in healthy subjects and patients with gait disorders.
A systematic review on time trend incidence of rheumatoid arthritis in outpatient rheumatology clinics
Authors: van Delft E. T. A. M. , Jamal Maha , den Braanker Hannah , Kuijper T. M. , Hazes J. M. W. , Lopes Barreto Deirisa , Weel-Koenders A. E. A. M.
Published: August 2022

Objectives

To classify patients with rheumatoid arthritis (RA) in an earlier stage of the disease, the ACR/EULAR classification criteria were updated in 2010. These criteria might have led to an increased incidence of RA in the rheumatology clinic. Since a higher incidence increases the socio-economic burden of RA, it is worthwhile to evaluate whether there is a time effect.

Materials and methods

A systematic review was conducted using Embase, Medline Ovid, Cochrane Central, and Web of Science from database inception to February 2021. Included were only articles that addressed incidence rates of rheumatoid arthritis from rheumatology outpatient clinics.

Results

Of the 6,289 publications only 243 publications on RA were found eligible for full-text review. Nine studies were included reporting incidence. The pooled incidence for RA was 11% (95% CI 6–16%) per year. Over time the incidence increased after the introduction of the 2010 ACR/EULAR classification criteria. Overall there was a high intragroup heterogeneity (I2 = 97.93%, p < 0.001), caused by geographical area, study design and differences in case definitions.

Conclusion

Although the incidence seems to increase after the introduction of the 2010 ACR/EULAR criteria, no conclusions can be drawn on this time effect due to heterogeneity.
Successful treatment of acute respiratory distress syndrome caused by hypervirulent Klebsiella pneumoniae with extracorporeal membrane oxygenation and continuous renal replacement therapy: A case report and literature review
Authors: Peng Wenzhong , Wu Yanhao , Lu Rongli , Zheng Yunpeng , Chen Jie , Pan Pinhua
Published: August 2022

Hypervirulent Klebsiella pneumoniae (hvKP) causes invasive infections and leads to high morbidity and mortality rates. Here, we report the case of a Chinese man with diabetes mellitus who developed acute respiratory distress syndrome and septic shock due to hvKP belonging to the K1 strain. The patient was treated with venovenous extracorporeal membrane oxygenation and continuous renal replacement therapy, in combination with antibiotics and recovered well. Clinicians should be aware of fatal infections caused by hvKP and investigate the best treatment options for patients at various stages of infection.
Prognostic value of chronicity grading on renal outcomes in patients with IgA nephropathy
Authors: Kang Donghyuk , Ban Tae Hyun , Chin Ho Jun , Lee Hajeong , Oh Se Won , Park Cheol Whee , Yang Chul Woo , Choi Bum Soon
Published: August 2022

Many studies have shown that chronic changes are strong predictors of renal outcomes in various kidney diseases, including IgA nephropathy. The Mayo Clinic/Renal Pathology Society suggested a glomerulonephritis reporting system with a proposal for standardized grading of chronic changes. The purpose of this study was to predict renal outcomes in patients with IgA nephropathy using chronicity grading in comparison to the Oxford classification which did not include global sclerosis. A total of 4,151 patients with IgA nephropathy were enrolled from the Korean GlomeruloNephritis Study Group registry. Chronicity grading was categorized into minimal, mild, moderate, and severe according to the extent of chronic changes. The Oxford T and S scores were considered as chronic lesions. Three prediction models were constructed: the Oxford classification model (Oxford S plus T), chronicity grading model A (chronicity grading), and chronicity grading model B (chronicity grading plus Oxford S). Using these three prediction models, the primary renal outcome (end-stage renal disease) was evaluated using Cox regression analysis and prediction performance. During the median follow-up of 6.1 (2.7–9.9) years, 304 (7.3%) patients progressed to end-stage renal disease with a cumulative incidence rate of 1.02 events per 100 person-years. In a fully adjusted multivariable model, chronicity grading was independently associated with the primary renal outcome in both models A and B. Compared to the Oxford model, both models A and B showed improvements in model fit, but not in discrimination (ΔC 0.001; 95% CI, −0.010 to 0.013 and ΔC 0.002; 95% CI, −0.005 to 0.008, respectively). Model B demonstrated improvements in integrated discrimination improvement (0.01; 95% CI, 0–0.03) and continuous net reclassification improvement (0.49; 95% CI, 0.02–0.72). The severity of chronicity grading is closely related to adverse renal outcomes in patients with IgA nephropathy, and chronicity grading could provide additional information in clinical practice alongside the Oxford classification.
Characteristics of gut microbiota of term small gestational age infants within 1 week and their relationship with neurodevelopment at 6 months
Authors: Chen Xiaona , Yan Zheng , Liu Lili , Zhang Rui , Zhang Xiaojiao , Peng Cheng , Geng Yuehang , Zhou Faliang , Han Ying , Hou Xinlin
Published: August 2022

Introduction

Small for gestational age (SGA) infants are at a higher risk of neurodevelopmental delay than infants appropriate for gestational age (AGA). Previous studies have confirmed that gut microbiota in early life influences subsequent neurodevelopment. However, few studies have reported corresponding data in SGA populations.

Objective

We aimed to evaluate the characteristics of the gut microbiota of term SGA infants and the associations between the gut microbiota in SGA infants and neurodevelopmental outcomes at 6 months of age.

Methods

Fecal samples were collected on days 1, 3, 5, and 7 from term SGA and AGA infants born between June 2020 and June 2021 at the Peking University First Hospital. 16S ribosomal deoxyribonucleic acid amplicon sequencing was used to analyze the fecal microbiota. We followed up for 6 months and used the Ages and Stages Questionnaires-3 (ASQ-3) to evaluate the neurodevelopmental outcomes among SGA infants.

Results

A total of 162 neonates were enrolled, with 41 SGA infants (25.3%) in the study group and 121 AGA infants (74.7%) in the control group. The gut microbial diversity in the SGA group was lower than that in the AGA group on days 1, 3, 5, and 7. Non-metric multidimensional scaling and analysis of similarities showed significant differences between the two groups. The SGA group had increased relative abundances of Ralstonia (3, 5, and 7 days) and Clostridium (3 and 7 days). The dominant microorganisms of the SGA group were Ralstonia on day 1, Escherichia_Shigella on days 3 and 7, and Clostridia on day 5. We found that the gut microbial diversity of SGA infants with poor communication scores was higher than that of SGA infants with good communication scores on day 3. Fine motor scores were negatively correlated with the relative abundance of Bacteroides_fragilis on day 1. A negative correlation was observed between gross motor scores and relative abundance of Clostridium_saccharobutylicum on day 7. Bacteroidota, Bacteroidia, Bacteroides, and Bacteroides_fragilis were the dominant microorganisms in the good communication score group on day 7. Communication scores were positively correlated with the relative abundance of Bacteroidota, Bacteroides, and Bacteroides_fragilis on day 7.

Conclusion

The gut microbial diversity of term SGA infants was significantly lower in the first week of life than that of term AGA infants. Certain pathogenic and conditional pathogenic bacteria, such as Escherichia_Shigella, Ralstonia and Clostridium increased or formed the dominant microbiota in SGA infants. Alpha diversity, Bacteroidota, Bacteroides, Bacteroides_fragilis, and Clostridium_saccharobutylicum found in SGA infants may be associated with neurodevelopmental outcomes at 6 months of age, indicating possible therapeutic targets for clinical intervention.
Total flavonoids of Abrus cantoniensis inhibit CD14/TLR4/NF-κB/MAPK pathway expression and improve gut microbiota disorders to reduce lipopolysaccharide-induced mastitis in mice
Authors: Sun Wen-Jing , Wu En-Yun , Zhang Ge-Yin , Xu Bai-Chang , Chen Xiao-Gang , Hao Kai-Yuan , Wang Ying , He Ling-Zhi , Lv Qi-Zhuang
Published: August 2022

Established a model of lipopolysaccharide (LPS)-induced mastitis in mice, pathological sections and myeloperoxidase were used to detect the degree of tissue damage, enzyme-linked immunosorbent assay (ELISA) was performed to detect the expression of pro-inflammatory cytokines, meanwhile fluorescence quantitative PCR experiments were performed to detect the mRNA expression of CD14/TLR4/NF-κB/MAPK signalling pathway, and the faeces of mice were collected for 16S measurement of flora. The results showed that Abrus cantoniensis total flavonoids (ATF) could significantly reduce the damage of LPS on mammary tissue in mice and inhibit the secretion of inflammatory factors such as TNF-α, IL-1β and IL-6. At the mRNA level, ATF inhibited the expression of CD14/TLR4/NF-κB/MAPK pathway and enhanced the expression of tight junction proteins in the blood-milk barrier. In the results of the intestinal flora assay, ATF were found to be able to regulate the relative abundance of the dominant flora from the phylum level to the genus level, restoring LPS-induced gut microbial dysbiosis. In summary, ATF attenuated the inflammatory response of LPS on mouse mammary gland by inhibiting the expression of CD14/TLR4/NF-κB/MAPK pathway, enhancing the expression of tight junction proteins and restoring LPS-induced gut microbial dysbiosis. This suggests that ATF could be a potential herbal remedy for mastitis.
Modeling the tonotopic map using a two-dimensional array of neural oscillators
Authors: Biswas Dipayan , Chakravarthy V. Srinivasa , Tarsode Asit
Published: August 2022

We present a model of a tonotopic map known as the Oscillatory Tonotopic Self-Organizing Map (OTSOM). It is a 2-dimensional, self-organizing array of Hopf oscillators, capable of performing a Fourier-like decomposition of the input signal. While the rows in the map encode the input phase, the columns encode frequency. Although Hopf oscillators exhibit resonance to a sinusoidal signal when there is a frequency match, there is no obvious way to also achieve phase tuning. We propose a simple method by which a pair of Hopf oscillators, unilaterally coupled through a coupling scheme termed as modified power coupling, can exhibit tuning to the phase offset of sinusoidal forcing input. The training of OTSOM is performed in 2 stages: while the frequency tuning is adapted in Stage 1, phase tuning is adapted in Stage 2. Earlier tonotopic map models have modeled frequency as an abstract parameter unconnected to any oscillation. By contrast, in OTSOM, frequency tuning emerges as a natural outcome of an underlying resonant process. The OTSOM model can possibly be regarded as an approximation of the tonotopic map found in the primary auditory cortices of mammals, particularly exemplified in the studies of echolocating bats.
Case report: Ultrasound-guided multi-site electroacupuncture stimulation for a patient with spinal cord injury
Authors: Song Xi-Zi , Chu Xiao-Lei , Liu Tao , Cao Yu-Tong , Li Rui-Xin , Gao Ming-Wei , Li Qing-Wen , Gu Xiao-Song , Ming Dong
Published: August 2022

Introduction

Spinal cord injury causes permanent neurological deficits, which have devastating physical, social, and vocational consequences for patients and their families. Traditional Chinese medicine uses acupuncture to treat neuropathic pain and improve nerve conduction velocity. This treatment can also reduce peripheral nerve injury joint contracture and muscle atrophy in affected patients. And it's got a remarkable restoration when electrical stimulation therapy on impaired peripheral nerves in animal models and clinical trials.

Case description

A 48-year-old woman was hit by a heavy object that injured her lower back. The patient had a T12-L1 vertebral flexion and stretch fracture with traumatic spinal stenosis. The patient was transferred to the rehabilitation department after posterior T12-L2-segment pedicle screw system distraction and reduction, internal fixation, decompression, and bone graft fusion. Ultrasound-guided electroacupuncture was used to stimulate the sacral nerve, the spinal nerve, and the head of the patient, accompanied by spinal joint loosening training, respiratory training, lumbar comprehensive sports training, paraplegic limbs comprehensive training, and other manipulative treatment.

Outcomes

After the intervention, the patient showed significant improvements in sensory and motor scores, resulting in functional recovery according to ASIA and FIM. The patient gradually showed reasonable functional remission.

Discussion

The sacral nerve, the spinal cord, and the head were electrically stimulated by ultrasound-guided electroacupuncture in terms of intervention, and various functions of the patient were alleviated to a certain extent. The efficacy of ultrasound-guided electroacupuncture stimulation in treating neurologic symptoms should be validated in future clinical trials.
Connectivity in deep brain stimulation for self-injurious behavior: multiple targets for a common network?
Authors: Heiden Petra , Weigel Daniel Tim , Loução Ricardo , Hamisch Christina , Gündüz Enes M. , Ruge Maximilian I. , Kuhn Jens , Visser-Vandewalle Veerle , Andrade Pablo
Published: August 2022

Self-injurious behavior (SIB) is associated with diverse psychiatric conditions. Sometimes (e.g., in patients with autism spectrum disorder or acquired brain injuries), SIB is the most dominant symptom, severely restricting the psychosocial functioning and quality of life of the patients and inhibiting appropriate patient care. In severe cases, it can lead to permanent physical injuries or even death. Primary therapy consists of medical treatment and if implementable, behavioral therapy. For patients with severe SIB refractory to conventional therapy, neuromodulation can be considered as a last recourse. In scientific literature, several successful lesioning and deep brain stimulation targets have been described that can indicate a common underlying neuronal pathway. The objectives of this study were to evaluate the short- and long-term clinical outcome of patients with severe, therapy refractory SIB who underwent DBS with diverse underlying psychiatric disorders and to correlate these outcomes with the activated connectivity networks. We retrospectively analyzed 10 patients with SIB who underwent DBS surgery with diverse psychiatric conditions including autism spectrum disorder, organic personality disorder after hypoxic or traumatic brain injury or Tourette syndrome. DBS targets were chosen according to the underlying disorder, patients were either stimulated in the nucleus accumbens, amygdala, posterior hypothalamus, medial thalamus or ventrolateral thalamus. Clinical outcome was measured 6 months after surgery and at long-term follow-up after 10 or more years using the Early Rehabilitation Barthel index (ERBI) and time of restraint. Connectivity patterns were analyzed using normative connectome. Based on previous literature the orbitofrontal cortex, superior frontal gyrus, the anterior cingulate cortex, the amygdala and the hippocampus were chosen as regions of interest. This analysis showed a significant improvement in the functionality of the patients with DBS in the short- and long-term follow-up. Good clinical outcome correlated with higher connectivity to the amygdala and hippocampus. These findings may suggest a common pathway, which can be relevant when planning a surgical procedure in patients with SIB.
Factors affecting two-point discrimination in Argus II patients
Authors: Yücel Ezgi I. , Sadeghi Roksana , Kartha Arathy , Montezuma Sandra Rocio , Dagnelie Gislin , Rokem Ariel , Boynton Geoffrey M. , Fine Ione , Beyeler Michael
Published: August 2022

Two of the main obstacles to the development of epiretinal prosthesis technology are electrodes that require current amplitudes above safety limits to reliably elicit percepts, and a failure to consistently elicit pattern vision. Here, we explored the causes of high current amplitude thresholds and poor spatial resolution within the Argus II epiretinal implant. We measured current amplitude thresholds and two-point discrimination (the ability to determine whether one or two electrodes had been stimulated) in 3 blind participants implanted with Argus II devices. Our data and simulations show that axonal stimulation, lift and retinal damage all play a role in reducing performance in the Argus 2, by either limiting sensitivity and/or reducing spatial resolution. Understanding the relative role of these various factors will be critical for developing and surgically implanting devices that can successfully subserve pattern vision.
Examining virtual driving test performance and its relationship to individuals with HIV-associated neurocognitive disorders
Authors: Grethlein David , Pirrone Vanessa , Devlin Kathryn N. , Dampier Will , Szep Zsofia , Winston Flaura K. , Ontañón Santiago , Walshe Elizabeth A. , Malone Kim , Tillman Shinika , Ances Beau M. , Kandadai Venk , Kolson Dennis L. , Wigdahl Brian
Published: August 2022

Significance

Existing screening tools for HIV-associated neurocognitive disorders (HAND) are often clinically impractical for detecting milder forms of impairment. The formal diagnosis of HAND requires an assessment of both cognition and impairment in activities of daily living (ADL). To address the critical need for identifying patients who may have disability associated with HAND, we implemented a low-cost screening tool, the Virtual Driving Test (VDT) platform, in a vulnerable cohort of people with HIV (PWH). The VDT presents an opportunity to cost-effectively screen for milder forms of impairment while providing practical guidance for a cognitively demanding ADL.

Objectives

We aimed to: (1) evaluate whether VDT performance variables were associated with a HAND diagnosis and if so; (2) systematically identify a manageable subset of variables for use in a future screening model for HAND. As a secondary objective, we examined the relative associations of identified variables with impairment within the individual domains used to diagnose HAND.

Methods

In a cross-sectional design, 62 PWH were recruited from an established HIV cohort and completed a comprehensive neuropsychological assessment (CNPA), followed by a self-directed VDT. Dichotomized diagnoses of HAND-specific impairment and impairment within each of the seven CNPA domains were ascertained. A systematic variable selection process was used to reduce the large amount of VDT data generated, to a smaller subset of VDT variables, estimated to be associated with HAND. In addition, we examined associations between the identified variables and impairment within each of the CNPA domains.

Results

More than half of the participants (N = 35) had a confirmed presence of HAND. A subset of twenty VDT performance variables was isolated and then ranked by the strength of its estimated associations with HAND. In addition, several variables within the final subset had statistically significant associations with impairment in motor function, executive function, and attention and working memory, consistent with previous research.

Conclusion

We identified a subset of VDT performance variables that are associated with HAND and assess relevant functional abilities among individuals with HAND. Additional research is required to develop and validate a predictive HAND screening model incorporating this subset.
Effectiveness of bimodal auditory and electrical stimulation in patients with tinnitus: A feasibility study
Authors: Spencer Shikha , Mielczarek Marzena , Olszewski Jurek , Sereda Magdalena , Joossen Iris , Vermeersch Hanne , Gilles Annick , Michiels Sarah
Published: August 2022

Background

Tinnitus is a common symptom, affecting about 10–15% of the adult population. When input from the somatosensory system can influence and/or elicit tinnitus, this type of subjective tinnitus is called somatosensory tinnitus. Recently, a new type of bimodal neurostimulation treatment has shown promising results for a specific subgroup within the somatosensory tinnitus population. It is, however, not clear if this bimodal stimulation is also effective in patients with other types of subjective tinnitus.

Aim

The aim of this study was to evaluate the feasibility and efficacy of non-invasive bimodal auditory-somatosensory stimulation in reducing tinnitus severity among a general population of people with subjective tinnitus.

Methods

Chronic subjective tinnitus patients were recruited from the ENT department of the Antwerp University Hospital. Somatosensory stimulation was delivered by Transcutaneous Electrical Nerve Stimulation (TENS), and it was combined with auditory stimulation via headphones. The therapy comprised six sessions of thirty minutes twice a week for a period of 3 consecutive weeks. Follow up measurements were scheduled 9–12 weeks after the last treatment session. The change of the Tinnitus Functional Index (TFI) score, a questionnaire evaluating tinnitus burden and effects on the quality of life, was the primary outcome measure.

Results

Twenty-nine patients were enrolled in the study. A linear mixed-effects model was used to analyze the efficacy of bimodal treatment. The results of this analysis showed a statistically significant decrease (by 6, 9 points) in average TFI score at the follow up visit when compared to baseline. The ability to modulate tinnitus did not have an influence on the treatment results.

Conclusion

Our study showed that bimodal stimulation is a feasible and safe method of tinnitus treatment. The method might be an effective treatment for some participants with tinnitus, especially those who have accompanying neck/temporomandibular problems, although, the evidence from this trial is quite weak. Additional research is needed toward establishing the optimal treatment protocol, as well as selecting the most appropriate inclusion criteria.
Amelioration effect of Lactobacillus plantarum KFY02 on low-fiber diet-induced constipation in mice by regulating gut microbiota
Authors: Yi Ruokun , Zhou Xin , Liu Tongji , Xue Rui , Yang Zhennai
Published: August 2022

This study aimed to examine the ameliorating effect of Lactobacillus plantarum (LP) KFY02 on low-fiber diet-induced constipation in mice. LP-KFY02 was isolated from the natural fermented yogurt in Korla of Xinjiang. The mice with low-fiber diet-induced constipation in experimental groups were administered 1 × 109 CFU/kg LP-KFY02 (KFY02H) and 1 × 108 CFU/kg LP-KFY02 (KFY02L). After LP-KFY02 treatment with constipation mice, the mice fecal water content, intestinal transit ability and defecation time of constipated mice were improved. The mice fecal flora diversity, abundance and structure of the intestinal flora were regulated to the balanced state. The mice serum levels of gut motility related neuroendocrine factors have been increased, the intestinal mucosal barrier function and gut motility related gene expression were regulated in mice colon tissues. At the same time, the mice colon tissue damage were improved. These parameters in the KFY02H group were close to the normal group. These results suggested that LP-KFY02 could be considered as a potential probiotic to help alleviate low-fiber diet-induced constipation. They also provided a theoretical basis for the study of probiotics to relieve constipation by regulating intestinal flora.
Model development and update of portable NIRS instrument for assessment of internal quality attributes of two navel orange varieties
Authors: Sun Xudong , Deng Di , Liu Jiacheng , Feng Shaoran
Published: August 2022

The variation of fruit among batches influences the performance of the portable near infrared spectroscopy (NIRS) instrument and then determines the success or failure for practical application in fruit industry. Model development and update methods were investigated for determining soluble solids contents (SSC) and titrable acidity (TA) of navel orange. The pretreatment and variable selection methods were explored for building partial least square regression (PLSR) models. The best models, developed by the combination of second derivative (2D) and variable sorting for normalization (VSN), could predict SSC but not TA. The root mean square error of prediction (RMSEP), coefficient of determination for prediction (Rp2) and ratio of prediction to deviation (RPD) for SSC were 0.66 °Brix, 0.66 and 1.73. Model maintain methods of model update (MU) and slope and bias correction (SBC) achieved the best results in predicting SSC for two external validation sets with Rp2, RMSEP and RPD of 0.54, 0.83 °Brix, 1.60 and 0.52, 0.83 °Brix, 1.65, respectively. The results suggested model development and update with MU and SBC could improve the robustness of the portable NIRS instrument in predicting SSC of navel orange.
Yuan-Zhi decoction in the treatment of Alzheimer’s disease: An integrated approach based on chemical profiling, network pharmacology, molecular docking and experimental evaluation
Authors: Wu Qiong , Li Xiang , Jiang Xiao-Wen , Yao Dong , Zhou Li-Jun , Xu Zi-Hua , Wang Nan , Zhao Qing-Chun , Zhang Zhou
Published: August 2022

Yuan-Zhi Decoction (YZD) is a traditional Chinese medical formulation with demonstrated clinical benefits in Alzheimer’s disease (AD). We used liquid chromatography coupled with mass spectrometry to identify 27 unique chemical components of YZD. Analyzing these using network pharmacology and molecular docking models identified 34 potential interacting molecular targets involved in 26 biochemical pathways. When tested in an animal model of AD, the APP/PS1 transgenic mice showed measurable improvements in spatial orientation and memory after the administration of YZD. These improvements coincided with significantly reduced deposition of Aβ plaques and tau protein in the hippocampi in the treated animals. In addition, a decreased BACE1 and beta-amyloid levels, a downregulation of the p-GSK-3β/GSK-3β, and an upregulation of the PI3K and p-AKT/AKT pathway was seen in YZD treated animals. These in vivo changes validated the involvement of molecular targets and pathways predicted in silico analysis of the chemical components of YZD. This study provides scientific support for the clinical use of YZD and justifies further investigations into its effects in AD. Furthermore, it demonstrates the utility of network pharmacology in elucidating the biochemical mechanisms underlying the beneficial effects of traditional Chinese medicines (TCM).
Component-Based Reduced Order Modeling of Large-Scale Complex Systems
Authors: Huang Cheng , Duraisamy Karthik , Merkle Charles
Published: August 2022

Large-scale engineering systems, such as propulsive engines, ship structures, and wind farms, feature complex, multi-scale interactions between multiple physical phenomena. Characterizing the operation and performance of such systems requires detailed computational models. Even with advances in modern computational capabilities, however, high-fidelity (e.g., large eddy) simulations of such a system remain out of reach. In this work, we develop a reduced‐order modeling framework to enable accurate predictions of large-scale systems. We target engineering systems which are difficult to simulate at a high-enough level of fidelity, but are decomposable into different components. These components can be modeled using a combination of strategies, such as reduced-order models (ROM) or reduced-fidelity full-order models (RF-FOM). Component-based training strategies are developed to construct ROMs for each individual component. These ROMs are then integrated to represent the full system. Notably, this approach only requires high-fidelity simulations of a much smaller computational domain. System-level responses are mimicked via external boundary forcing during training. Model reduction is accomplished using model-form preserving least-squares projections with variable transformation (MP-LSVT) (Huang et al., Journal of Computational Physics, 2022, 448: 110742). Predictive capabilities are greatly enhanced by developing adaptive bases which are locally linear in time. The trained ROMs are then coupled and integrated into the framework to model the full large-scale system. We apply the methodology to extremely complex flow physics involving combustion dynamics. With the use of the adaptive basis, the framework is demonstrated to accurately predict local pressure oscillations, time-averaged and RMS fields of target state variables, even with geometric changes.
Janus van der waals equations for real molecules with two-sided phase transitions
Authors: Kim Jihwan , Kim Do-Hyun , Park Jeong-Hyuck
Published: August 2022

We obtained families of generalized van der Waals equations characterized by an even number n = 2, 4, 6 and a continuous free parameter, which is tunable for a critical compressibility factor. Each equation features two adjacent critical points which have a common critical temperature Tc and arbitrarily two close critical densities. The critical phase transitions are naturally two-sided: the critical exponents are αP=γP=23 and βP=δ−1=13 for T > Tc and αP=γP=nn+1 and βP=δ−1=1n+1 for T < Tc. In contrast with the original van der Waals equation, our novel equations all reduce consistently to the classical ideal gas law in the low-density limit. We tested our formulas against the NIST data for 11 major molecules and showed agreements better than the original van der Waals equation, not only near to the critical points but also in low-density regions.
EEG artifact removal using sub-space decomposition, nonlinear dynamics, stationary wavelet transform and machine learning algorithms
Authors: Zangeneh Soroush Morteza , Tahvilian Parisa , Nasirpour Mohammad Hossein , Maghooli Keivan , Sadeghniiat-Haghighi Khosro , Vahid Harandi Sepide , Abdollahi Zeinab , Ghazizadeh Ali , Jafarnia Dabanloo Nader
Published: August 2022

Blind source separation (BSS) methods have received a great deal of attention in electroencephalogram (EEG) artifact elimination as they are routine and standard signal processing tools to remove artifacts and reserve desired neural information. On the other hand, a classifier should follow BSS methods to automatically identify artifactual sources and remove them in the following steps. In addition, removing all detected artifactual components leads to loss of information since some desired information related to neural activity leaks to these sources. So, an approach should be employed to detect and suppress the artifacts and reserve neural activity. This study introduces a novel method based on EEG and Poincare planes in the phase space to detect artifactual components estimated by second-order blind identification (SOBI). Artifacts are detected using a mixture of well-known conventional classifiers and were removed employing stationary wavelet transform (SWT) to reserve neural information. The proposed method is a combination of signal processing techniques and machine learning algorithms, including multi-layer perceptron (MLP), K-nearest neighbor (KNN), naïve Bayes, and support vector machine (SVM) which have significant results while applying our proposed method to different scenarios. Simulated, semi-simulated, and real EEG signals are employed to evaluate the proposed method, and several evaluation criteria are calculated. We achieved acceptable results, for example, 98% average accuracy and 97% average sensitivity in artifactual EEG component detection or about 2% as mean square error in EEG reconstruction after artifact removal. Results showed that the proposed method is effective and can be used in future studies as we have considered different real-world scenarios to evaluate it.
Validity of the peak velocity to detect physical training improvements in athymic mice
Authors: Kraemer Maurício Beitia , Silva Karen Christine , Kraemer Camila Cunha França , Pereira Juliana Silva , dos Reis Ivan Gustavo Masseli , Priolli Denise Gonçalves , Messias Leonardo Henrique Dalcheco
Published: August 2022

This study comprises two complementary experiments with athymic Balb/c (Nu/Nu) mice. In experiment 1, the aim was to verify the reproducibility of the peak velocity (VPeak) determined from the incremental test. The second experiment aimed to assess the VPeak sensitivity to prescribe and detect modulations of the physical training in athymic nude mice. Sixteen mice were submitted to two incremental treadmill tests separated by 48-h (Experiment 1). The test consisted of an initial warm-up of 5 minutes. Subsequently, animals initiated the tests at 8 m min−1 with increments of 2 m min−1 every 3 minutes. The VPeak was determined as the highest velocity attained during the protocol. In experiment 2, these animals were randomly allocated to an exercise group (EG) or a control group (CG). The training protocol consisted of 30-min of treadmill running at 70% of the VPeak five times a week for 4 weeks. High indexes of reproducibility were obtained for VPeak (Test = 19.7 ± 3.6 m min−1; Retest = 19.2 ± 3.4 m min−1; p = 0.171; effect size = 0.142; r = 0.90). Animals from the EG had a significant increase of VPeak (Before = 18.4 ± 2.7 m min−1; After = 24.2 ± 6.0 m min−1; p = 0.023). Conversely, a significant decrease was observed for the CG (Before = 21.1 ± 3.9 m min−1; After = 15.9 ± 2.7 m min−1; p = 0.038). The VPeak is a valid parameter for exercise prescription in studies involving athymic nude mice.
A sensitive mitochondrial thermometry 2.0 and the availability of thermogenic capacity of brown adipocyte
Authors: Meng Xiao-Yan , Wang Dian-Dian , Xie Tao-Rong , Yang Run-Zhou , Liu Chun-Feng , Liu Dan-Hua , Li Shu-Ang , Luan Yi , Kang Jian-Sheng
Published: August 2022

The temperature of a living cell is a crucial parameter for cellular events, such as cell division, gene expressions, enzyme activities and metabolism. We previously developed a quantifiable mitochondrial thermometry 1.0 based on rhodamine B methyl ester (RhB-ME) and rhodamine 800 (Rh800), and the theory for mitochondrial thermogenesis. Given that the synthesized RhB-ME is not readily available, thus, a convenient mitochondrial thermometry 2.0 based on tetra-methyl rhodamine methyl ester (TMRM) and Rh800 for the thermogenic study of brown adipocyte was further evolved. The fluorescence of TMRM is more sensitive (∼1.4 times) to temperature than that of RhB-ME, then the TMRM-based mito-thermometry 2.0 was validated and used for the qualitatively dynamic profiles for mitochondrial thermogenic responses and mitochondrial membrane potential in living cells simultaneously. Furthermore, our results demonstrated that the heterogenous thermogenesis evoked by β3 adrenoceptor agonist only used overall up to ∼46% of the thermogenic capacity evoked by CCCP stimulation. On the other hand, the results demonstrated that the maximum thermogenesis evoked by NE and oligomycin A used up to ∼79% of the thermogenic capacity, which suggested the maximum thermogenic capacity under physiological conditions by inhibiting the proton-ATPase function of the mitochondrial complex V, such as under the cold activation of sympathetic nerve and the co-release of sympathetic transmitters.
Distinct rhizosphere soil responses to nitrogen in relation to microbial biomass and community composition at initial flowering stages of alfalfa cultivars
Authors: An Yunru , Sun Haoyang , Zhang Wei , Sun Yunfu , Li Shuxia , Yu Zhouchang , Yang Rongchen , Hu Tianming , Yang Peizhi
Published: August 2022

In the long-term growth process, alfalfa rhizosphere forms specific microbiome to provide nutrition for its growth and development. However, the effects of different perennial alfalfa cultivars on changes in the rhizosphere soil characteristics and microbiome are not well understood. In this study, 12 perennial alfalfa cultivars were grown continuously for eight years. Rhizosphere samples were tested using Illumina sequencing of the 16S rRNA gene coupled with co-occurrence network analysis to explore the relationship between alfalfa (biomass and crude protein content), soil properties, and the microbial composition and diversity. Redundancy analysis showed SOC and pH had the greatest impact on the composition of the rhizosphere microbial community. Moreover, microbial diversity also contributes to microbial composition. Soil properties (AP, EC, SOC and pH) exhibited a significant positive correlation with soil bacterial communities, which was attributed to the differences between plant cultivars. Partial least squares path modeling (PLS-PM) revealed that microbial biomass and community composition rather than diversity, are the dominant determinants in the rhizosphere soil nitrogen content of perennial alfalfa. Our findings demonstrate that the soil microbial biomass and composition of rhizosphere bacterial communities are strongly affected by cultivar, driving the changes in soil nitrogen content, and variances in the selective capacities of plants.
Experimental evaluation of biological regeneration of arable soil: The effects of grass-clover leys and arbuscular mycorrhizal inoculants on wheat growth, yield, and shoot pathology
Authors: Austen Nichola , Tille Stefanie , Berdeni Despina , Firbank Leslie G. , Lappage Martin , Nelson Michaela , Helgason Thorunn , Marshall-Harries Ewan , Hughes H. Bleddyn , Summers Richard , Cameron Duncan D. , Leake Jonathan R.
Published: August 2022

Wheat yields have plateaued in the UK over the last 25 years, during which time most arable land has been annually cropped continuously with short rotations dominated by cereals. Arable intensification has depleted soil organic matter and biology, including mycorrhizas, which are affected by tillage, herbicides, and crop genotype. Here, we test whether winter wheat yields, mycorrhization, and shoot health can be improved simply by adopting less intensive tillage and adding commercial mycorrhizal inoculum to long-term arable fields, or if 3-year grass-clover leys followed direct drilling is more effective for biological regeneration of soil with reduced N fertiliser. We report a trial of mycorrhization, ear pathology, and yield performance of the parents and four double haploid lines from the Avalon x Cadenza winter wheat population in a long-term arable field that is divided into replicated treatment plots. These plots comprised wheat lines grown using ploughing or disc cultivation for 3 years, half of which received annual additions of commercial arbuscular mycorrhizal (AM) inoculum, compared to 3-year mown grass-clover ley plots treated with glyphosate and direct-drilled. All plots annually received 35 kg of N ha−1 fertiliser without fungicides. The wheat lines did not differ in mycorrhization, which averaged only 34% and 40% of root length colonised (RLC) in the ploughed and disc-cultivated plots, respectively, and decreased with inoculation. In the ley, RLC increased to 52%. Two wheat lines were very susceptible to a sooty ear mould, which was lowest in the ley, and highest with disc cultivation. AM inoculation reduced ear infections by >50% in the susceptible lines. In the ley, yields ranged from 7.2 to 8.3 t ha−1, achieving 92 to 106% of UK average wheat yield in 2018 (7.8 t ha−1) but using only 25% of average N fertiliser. Yields with ploughing and disc cultivation averaged only 3.9 and 3.4 t ha−1, respectively, with AM inoculum reducing yields from 4.3 to 3.5 t ha−1 in ploughed plots, with no effect of disc cultivation. The findings reveal multiple benefits of reintegrating legume-rich leys into arable rotations as part of a strategy to regenerate soil quality and wheat crop health, reduce dependence on nitrogen fertilisers, enhance mycorrhization, and achieve good yields.
Investigating the detection of parent-child relationships in early childhood: The role of partiality in resource distributions
Authors: McPhee Anna Michelle , Bagh Sinamys , Schmuckler Mark A. , Sommerville Jessica A.
Published: August 2022

By early childhood, children possess clear expectations about how resources should be, and typically are, distributed, expecting and advocating for equal resource distributions to recipients. Moreover, recent evidence suggests that children may be able to use deviations from equality in resource distributions to make inferences about the nature of social relationships. Here, we investigated whether children use partiality in resource distributions displayed by adults toward children in third-party contexts to identify parent-child relationships, whether children anticipate preferential treatment based upon knowledge of third-party parent-child relationships, and whether children anticipate different emotional reactions to impartiality in resource distributions in parent-child interactions compared to neighbor-child interactions. Four-to seven-year-old children were presented with hypothetical vignettes about an adult character who distributed resources to two children either equally, or systematically favoring one child. By the age of 4, children used resource distribution partiality to identify an adult as a child’s parent, and also used these expectations to guide their anticipated emotional reactions to impartiality. By the age of 6, children were also more likely to anticipate partiality to be displayed in parent-child compared to neighbor-child relationships. The findings from the current study reveal that partiality in resource distributions acts as a valuable cue to aid in identifying and understanding social relationships, highlighting the integral role that resources play in children’s understanding of their social world. More broadly, our findings support the claim that children use cues that signal interpersonal investment to specify and evaluate parent-child relationships in third-party contexts.
Nexus between social responsibilities of young cinematic celebrities and public recognition: Evidence from China
Authors: Zhao Yun , Eyraud Tina , Xue Yaowen , Imran Muhammad , Wang Kai , Sun Xiaotong
Published: August 2022

As public figures, cinematic celebrities’ behaviors have widespread influence on the whole society, and this also applies to China. Their influence is reflected in public recognition. This research classifies celebrity social responsibility as behavior within the law, professionalism, family ethics, public morality, and charity. We selected 286 cinematic celebrities as study objects and obtained public recognition data through 2,600 questionnaire surveys. The findings of the study indicate that there is a positive, linear relationship between cinematic celebrities’ social responsibility and public recognition. In particular, family ethics and public moral responsibility demonstrate a significant positive correlation with public recognition. However, there was no significant correlation found between lawful responsibility and public recognition. Finally, this paper makes additional suggestions and recommendations drawn from the data and reported in the conclusion.
The impact of website quality on customer satisfaction and eWOM in online purchase intention: The moderating role of gender in risk-taking
Authors: Saleem Uzman , yi Su , Bilal Muhammad , Topor Dan Ioan , Căpuṣneanu Sorinel
Published: August 2022

Recently, social media marketing has become one of the most significant growth channels for many businesses. However, many companies are still unclear about using social media marketing to their advantage, particularly in an e-commerce environment. In this background, this study examines the effect of website quality, consumer satisfaction, and eWOM on online purchase intention. An online survey was conducted with 789 online Chinese shoppers from four cities—Harbin, Shenyang, Guangzhou, and Shenzhen. Structural equation modeling (SEM) was used to analyze the hypotheses. The findings show that each variable had a high impact on eWOM with website quality (information quality, system quality, and service quality), which in turn positively increased consumer online purchase intentions in China's e-commerce business. Additionally, findings show a significant gender gap in online shopping behavior. This novel research provides several managerial guidelines that support managers in improving their business performance in the e-commerce industry. This research also highlighted some limitations.