bluuebunny/crossref_metadata_2025 huggingface dataset:
Crafting Attributes of a Geological Now
Authors: Bohle Martin
Published: December 2023

Taking an Earth Science framework as its point of departure, this paper delves into the sociocultural ramifications of two distinct geological interpretations—epoch and event—of the contested but widely acknowledged term “Anthropocene.” While the term has gained considerable traction for encapsulating planetary-scale anthropogenic changes (PSAC), its meanings are varied, spanning scientific, philosophical, and cultural discourses. Earth scientists often examine PSAC through the lens of their discipline, yet the complex sociocultural implications attached to such geoscientific concepts call for an interdisciplinary perspective. Introduced by Crutzen and Stoermer in 2000, the term “Anthropocene” is designed to account for the transformative impact of modern societies on Earth System dynamics. Presently, two opposing viewpoints exist within geological discourse to describe PSAC: the “Anthropocene-as-an-event” concept, which underscores the accretion of human-induced changes, and the “Anthropocene-as-an-epoch” concept, focusing on a substantial alteration in Earth System dynamics. Utilising Renn’s theory of “The Evolution of Knowledge,” the paper posits these concepts as scientific borderline problems, catalysts for epistemic inquiries, linked to specific “economies of knowledge.” The ensuing analysis accentuates the multidimensionality of articulating PSAC from an Earth Science viewpoint, asserting that it not only mandates understanding the geological dimensions but also calls for integrating sociocultural and philosophical contexts. The paper concludes that geological terminology should consider associated cultural contexts of Earth Science to establish leadership for vital epistemic concepts.
Reducing the prosthesis modulus by inclusion of an open space lattice improves osteogenic response in a sheep model of extraarticular defect
Authors: Sanaei Reza , Pagel Charles Neil , Ayodele Babatunde A. , Lozanovski Bill , Beths Thierry , Leary Martin , Shidid Darpan , Kastrati Endri , Elambasseril Joe , Bühner Ulrich , Williamson Tom , Ryan Stewart , Brandt Milan
Published: December 2023

Introduction: Stress shielding is a common complication following endoprosthetic reconstruction surgery. The resulting periprosthetic osteopenia often manifests as catastrophic fractures and can significantly limit future treatment options. It has been long known that bone plates with lower elastic moduli are key to reducing the risk of stress shielding in orthopedics. Inclusion of open space lattices in metal endoprostheses is believed to reduce the prosthesis modulus potentially improving stress shielding. However, no in vivo data is currently available to support this assumption in long bone reconstruction. This manuscript aims to address this hypothesis using a sheep model of extraarticular bone defect.

Methods: Initially, CT was used to create a virtual resection plan of the distal femoral metaphyses and to custom design endoprostheses specific to each femur. The endoprostheses comprised additively manufactured Ti6Al4V-ELI modules that either had a solid core with a modulus of ∼120 GPa (solid implant group) or an open space lattice core with unit cells that had a modulus of 3–6 GPa (lattice implant group). Osteotomies were performed using computer-assisted navigation followed by implantations. The periprosthetic, interfacial and interstitial regions of interest were evaluated by a combination of micro-CT, back-scattered scanning electron microscopy (BSEM), as well as epifluorescence and brightfield microscopy.

Results: In the periprosthetic region, mean pixel intensity (a proxy for tissue mineral density in BSEM) in the caudal cortex was found to be higher in the lattice implant group. This was complemented by BSEM derived porosity being lower in the lattice implant group in both caudal and cranial cortices. In the interfacial and interstitial regions, most pronounced differences were observed in the axial interfacial perimeter where the solid implant group had greater bone coverage. In contrast, the lattice group had a greater coverage in the cranial interfacial region.

Conclusion: Our findings suggest that reducing the prosthesis modulus by inclusion of an open-space lattice in its design has a positive effect on bone material and morphological parameters particularly within the periprosthetic regions. Improved mechanics appears to also have a measurable effect on the interfacial osteogenic response and osteointegration.
New insight of the pathogenesis in osteoarthritis: the intricate interplay of ferroptosis and autophagy mediated by mitophagy/chaperone-mediated autophagy
Authors: An Fangyu , Zhang Jie , Gao Peng , Xiao Zhipan , Chang Weirong , Song Jiayi , Wang Yujie , Ma Haizhen , Zhang Rui , Chen Zhendong , Yan Chunlu
Published: December 2023

Ferroptosis, characterized by iron accumulation and lipid peroxidation, is a form of iron-driven cell death. Mitophagy is a type of selective autophagy, where degradation of damaged mitochondria is the key mechanism for maintaining mitochondrial homeostasis. Additionally, Chaperone-mediated autophagy (CMA) is a biological process that transports individual cytoplasmic proteins to lysosomes for degradation through companion molecules such as heat shock proteins. Research has demonstrated the involvement of ferroptosis, mitophagy, and CMA in the pathological progression of Osteoarthritis (OA). Furthermore, research has indicated a significant correlation between alterations in the expression of reactive oxygen species (ROS), adenosine monophosphate (AMP)-activated protein kinase (AMPK), and hypoxia-inducible factors (HIFs) and the occurrence of OA, particularly in relation to ferroptosis and mitophagy. In light of these findings, our study aims to assess the regulatory functions of ferroptosis and mitophagy/CMA in the pathogenesis of OA. Additionally, we propose a mechanism of crosstalk between ferroptosis and mitophagy, while also examining potential pharmacological interventions for targeted therapy in OA. Ultimately, our research endeavors to offer novel insights and directions for the prevention and treatment of OA.
Causal effect of gut microbiota on Gastroduodenal ulcer: a two-sample Mendelian randomization study
Authors: Zhang Jing , Hu Yingqiu , Wu Lidong , Zeng Qi , Hu Bin , Luo Zhiqiang , Wang Yibing
Published: December 2023

Background

Gastroduodenal ulcers are associated with Helicobacter pylori infection and the use of nonsteroidal anti-inflammatory drugs (NSAIDs). However, the causal relationship between gastroduodenal ulcers and gut microbiota, especially specific gut microbiota, remains unclear.

Methods

We conducted an analysis of published data on the gut microbiota and Gastroduodenal ulcer using genome-wide association studies (GWAS). Two-sample Mendelian randomization (MR) analysis was performed to determine the causal relationship between gut microbiota and Gastroduodenal ulcer. Sensitivity, heterogeneity, and pleiotropy analyses were conducted to confirm the accuracy of the research findings.

Results

Our study showed that the abundance of Enterobacteriaceae, Butyricicoccus, Candidatus Soleaferrea, Lachnospiraceae NC2004 group, Peptococcus, and Enterobacteriales was negatively correlated with the risk of Gastroduodenal ulcer. Conversely, the abundance of Streptococcaceae, Lachnospiraceae UCG010, Marvinbryantia, Roseburia, Streptococcus, Mollicutes RF9, and NB1n was positively correlated with the risk of Gastroduodenal ulcer. MR analysis revealed causal relationships between 13 bacterial genera and Gastroduodenal ulcer.

Conclusion

This study represents a groundbreaking endeavor by furnishing preliminary evidence regarding the potentially advantageous or detrimental causal link between the gut microbiota and Gastroduodenal ulcer, employing Mendelian Randomization (MR) analysis for the first time. These discoveries have the potential to yield fresh perspectives on the prevention and therapeutic approaches concerning Gastroduodenal ulcer, with a specific focus on the modulation of the gut microbiota.
The immune response in canine and human leishmaniasis and how this influences the diagnosis- a review and assessment of recent research
Authors: Ivănescu Larisa , Andronic Bianca Lavinia , Grigore-Hristodorescu Smaranda , Martinescu Gabriela Victoria , Mîndru Raluca , Miron Liviu
Published: December 2023

Leishmaniasis is a widespread but still underdiagnosed parasitic disease that affects both humans and animals. There are at least 20 pathogenic species of Leishmania, most of them being zoonotic. The diagnosis of leishmaniasis remains a major challenge, with an important role being played by the species of parasites involved, the genetic background, the immunocompetence of the host. This paper brings to the fore the sensitivity of the balance in canine and human leishmaniasis and addresses the importance of the host’s immune response in establishing a correct diagnosis, especially in certain cases of asymptomatic leishmaniasis, or in the situation the host is immunosuppressed or acquired leishmaniasis through vertical transmission. The methods considered as a reference in the diagnosis of leishmaniasis no longer present certainty, the diagnosis being influenced mostly by the immune response of the host, which differs according to the presence of other associated diseases or even according to the breed in dogs. Consequently, the diagnosis and surveillance of leishmaniasis cases remains an open topic, requiring new diagnostic methods adapted to the immunological state of the host.
Association between intraoperative hyperglycemia/hyperlactatemia and acute kidney injury following on-pump cardiac surgery: a retrospective cohort study
Authors: He Qiyu , Tan Zhimin , Chen Dongxu , Cai Shuang , Zhou Leng
Published: December 2023

Background

Despite the long-lasting notion about the substantial contribution of intraoperative un-stabilization of homeostasis factors on the incidence on acute kidney injury (AKI), the possible influence of intraoperative glucose or lactate management, as a modifiable factor, on the development of AKI remains inconclusive.

Objectives

To investigated the relationship between intraoperative hyperglycemia, hyperlactatemia, and postoperative AKI in cardiac surgery.

Methods

A retrospective cohort study was conducted among 4,435 adult patients who underwent on-pump cardiac surgery from July 2019 to March 2022. Intraoperative hyperglycemia and hyperlactatemia were defined as blood glucose levels >10 mmol/L and lactate levels >2 mmol/L, respectively. The primary outcome was the incidence of AKI. All statistical analyses, including t tests, Wilcoxon rank sum tests, chi-square tests, Fisher's exact test, Kolmogorov-Smirnov test, logistic regression models, subgroup analyses, collinearity analysis, and receiver operating characteristic analysis, were performed using the statistical software program R version 4.1.1.

Results

Among the 4,435 patients in the ﬁnal analysis, a total of 734 (16.55%) patients developed AKI after on-pump cardiac surgery. All studied intraoperative metabolic disorders was associated with increased AKI risk, with most pronounced odds ratio (OR) noted for both hyperglycemia and hyperlactatemia were present intraoperatively [adjusted OR 3.69, 95% confidence intervals (CI) 2.68–5.13, p < 0.001]. Even when hyperglycemia or hyperlactatemia was present alone, the risk of postoperative AKI remained elevated (adjusted OR 1.97, 95% CI 1.50–2.60, p < 0.001).

Conclusion

The presence of intraoperative hyperglycemia and hyperlactatemia may be associated with postoperative acute kidney injury (AKI) in patients undergoing on-pump cardiac surgery. Proper and timely interventions for these metabolic disorders are crucially important in mitigating the risk of AKI.
Acoustics and the well-being of children and personnel in early childhood education and care
Authors: Martikainen Silja , Prawda Karolina , Ståhlberg-Aalto Freja , Lautanala Ida , Kostilainen Kaisamari , Välimäki Vesa , Tervaniemi Mari
Published: December 2023

Studies implementing a multimethod perspective in evaluating the acoustics of early childhood education and care (ECEC) spaces both quantitatively and qualitatively are still scarce. In this study the acoustic environments (noise levels and reverberation times) of seven Finnish ECEC group’s premises were examined in association with personnel’s (N = 22) and children’s (N = 71) well-being. Personnel’s well-being and vocal health and children’s well-being were assessed with questionnaires. The findings were further elaborated by documentation of the ECEC spaces and semi-structured interviews with the ECEC personnel detailing their views on the acoustic environment of the daycare buildings and how and if the acoustics should be improved. The results showed that noise exceeding 70 dB affected personnel’s vocal health negatively, whereas no associations were found regarding acoustics and children’s or personnel’s well-being. Based on the interviews, sound spreading, poor insulation, and hard surfaces add to negative experiences of noisiness. ECEC groups need spaces that can be closed and acoustically separated from each other and from other groups. The possibility to close a space supports the perceived well-being of the users and provides a more varied and individualized use of the spaces.
Developing a digital field notebook for bioscience students in higher education
Authors: Maddison Janine , Bevan Richard Martin , Marsham Sara
Published: December 2023

Introduction

The use of mobile device presents both benefits and barriers. However, studies into the use of technology in fieldwork often focus only on either practitioner views or student views. Digital field notebooks (DFNs) are one-way mobile devices can be used to enhance fieldwork. Yet their use is limited to Geography, Earth and Environmental Science (GEES) disciplines, with students often playing a passive role during the development of DFNs. This research reports on the development of a DFN to enhance bioscience fieldwork in Higher Education (HE).

Methods

Using interviews, focus groups, and survey methods we investigated how both fieldwork practitioners and learners view the role of technology in the field. Working in partnership with students, we explored their experiences of using a DFN during fieldwork. Feedback was utilized to make changes to the DFN to support its integration within bioscience fieldwork.

Results

Overall, valuable developments related to content, technology, and pedagogy were made to the DFN, identifying value in a co-creation process. For example, students suggested the role of the DFN as a collaborative tool where individual entries were collated together. A workflow schematic and case study are presented for how a DFN can be used during bioscience fieldwork in HE.

Discussion

Although students identified place connection and the development of reflective practice as particular affordances, students did not identify any digital skill development opportunities when using the DFN. Additionally, although students suggested the DFN was easy to use, barriers remain for students in using a DFN. We suggest further research on the complex issues of permission and perceptions of value of mobile device use during fieldwork. Additionally, more explicit reference to digital skill developments should be made when using a DFN.
Evaluating the impact of conceptual change pedagogy on student attitudes and behaviors toward controversial topics in Iraq
Authors: Rea-Ramirez Mary Anne , Abboud Lena , Ramirez Tina
Published: December 2023

This study assesses the effect of conceptual change pedagogy on students’ attitudes toward pluralism and related rights within culturally sensitive contexts. Global efforts to address the spread of intolerant ideologies that foment radicalization, discrimination, and violence are fraught with controversy. Prior research on the Middle East and North Africa region has found that efforts to address these challenges in the field of education — including reform to curricula, the promotion of narratives inclusive of religious diversity, and civics education initiatives — have had varied levels of success. Absent from these efforts is the development of an effective pedagogy and the training of teachers to identify and address ideologies and behaviors that foment intolerance and conflict among students. Hardwired Global developed a teacher training program based on conceptual change theory and pedagogy to fill these needs. Conceptual change refers to the development of new ways of thinking and understanding of concepts, beliefs, and attitudes. Hardwired Global implemented the program in partnership with the regional Directorate of Education for Mosul and the Nineveh Plains region of Iraq from 2019–2023. From 2021–2023, Hardwired trained 485 teachers in 40 schools across the region. Following the training, teachers implemented two lessons. A mixed method research study — with a primary focus on quantitative data collected — was conducted to determine the effect of the program on student perceptions, understanding, and behavior toward key concepts inherent to pluralism. Quantitative data consisted of a pre-post survey with four multiple choice questions. Scores on pre-surveys were compared to post-surveys and a two sample paired t-test was applied. We documented statistically significant developments in students’ conceptual understanding of key concepts inherent to pluralism and associated rights, including: respect for diversity in expression inclusion of diverse religious and/or ethnic communities, gender equality, and violent or non-violent approaches to conflict. Qualitative data consisted of semi-structured interviews with teachers and students implemented at the conclusion of the program and observations reported by Master Trainers and teachers during training and activity implementation. Findings suggest conceptual change pedagogy on pluralism and associated rights is a promising approach to education about controversial topics in conflict-affected and culturally sensitive environments.
Aesthetical entanglements in mathematics education
Authors: Nemirovsky Ricardo , Kathotia Vinay , Mégrourèche Charlotte
Published: December 2023

In this study, we develop a perspective on the diverse aesthetics historically associated with mathematics, inspired by Rancière's approach to aesthetics and politics. We call “Silencing Aesthetics” a dominant aesthetic that Rota has characterized as a “copout (…) intended to keep our formal description of mathematics as close as possible to the description of a mechanism”. The challenge this study attempts to explore is how to question silencing aesthetics to make space for inclusive ones. Our efforts have focused on setting up and studying inclusive and pluralist “Studios”, gathering craftworkers, anthropologists, mathematics educators, and mathematics enthusiasts. We include here a case study based on a conversation amongst basket weavers, anthropologists, and mathematics educators focused on the artisanal and mathematical nature of knots. We discuss the implications of aesthetical entanglements, such as those in our case study, for mathematics learning.
Early onset age increases the risk of musculoskeletal damage in patients with type 2 diabetes
Authors: Zheng Biao , Zhang Yongze , Huang Lingning , Shen Ximei , Zhao Fengying , Yan Sunjie
Published: December 2023

Introduction

It’s not clear whether there are differences in musculoskeletal damage and body composition among different age groups of type 2 diabetes. Therefore, the purpose of this study is to analyze the difference between early-onset type 2 diabetes (EOT2D) and non-early-onset type 2 diabetes (NOT2D) in musculoskeletal damage.

Methods

A total of 964 patients with type 2 diabetes mellitus were selected by 1:1 propensity score matching, including 534 males and 430 females, with an average age of 52 ± 7 years and an average course of 10 ± 8.5 years. Bone mineral density and body composition were measured, and combined with biochemical tests, linear regression and binary logic regression were used to analyze the relationship between EOT2D, NOT2D and musculoskeletal damage. In addition, 414 patients with T2DM were selected according to whether they were hospitalized twice or not, and the median follow-up period was 44 months. COX survival analysis further elucidates the relationship between EOT2D, NOT2D and musculoskeletal damage.

Results

Compared with patients with non-early-onset type 2 diabetes, A/G was negatively correlated with the age of onset, and had statistical significance. EOT2D has a higher risk of sarcopenia, osteoporosis and even musculoskeletal damage. With the prolongation of the course of the disease, the risk of muscle mass and/or bone mineral density decrease in EOT2D increases.

Conclusion

EOT2D brings a greater risk of sarcopenia and/or osteoporosis, as well as a higher risk of reduced ASM and BMD. In addition, fat distribution may be more central.
Source or decomposition of soil organic matter: what is more important with increasing forest age in a subalpine setting?
Authors: Speckert Tatjana Carina , Wiesenberg Guido L. B.
Published: December 2023

Afforestation has been the dominant land-use change in the Swiss Alps during the last decades which has not only the potential to increase soil organic carbon sequestration, but it has also the potential to alter soil organic matter (SOM) dynamics through the vegetation shift and change in organic matter (OM) input into soils. The effects of afforestation on SOM dynamics, however, are still not fully understood as specific sources of OM and modifications of soil processes influencing decomposition and preservation remain largely unknown on alpine to subalpine slopes. Within this study we aimed to identify the potential sources and the decomposition of OM in a subalpine afforestation chrono-sequence (0–130 years) with Norway spruce (Picea abies L.) on a former pasture by using a multi-proxy molecular marker approach. We observed that leaf-derived OM plays an essential role in the pasture areas, while root-derived OM only plays a minor role in pasture and forest areas. Needle-derived OM represents the dominant source of SOM with increasing forest age, while understory shrubs and moss also contribute to the OM input in younger forest stand ages. However, needle litter and buildup of organic layers and subsequently less input of fresh OM from organic horizons to mineral soil can result in increased OM decomposition in mineral soils rather than contributing to additional SOM stabilization in mineral soils. This was most pronounced in the oldest forest stand (130-year-old) in the investigated afforestation sequence, particularly in deeper soil horizons (10–45 cm). Thereby, our study provides new insights into SOM dynamics following afforestation, especially with respect to the long-term SOM sequestration potential of afforestation of subalpine pasture soils.
Metabolic changes with the occurrence of atherosclerotic plaques and the effects of statins
Authors: Zhao Lingmin , Ma Di , Wang LiJuan , Su XingQi , Feng LiangShu , Zhu LiChong , Chen Ying , Hao YuLei , Wang XinYu , Feng JiaChun
Published: December 2023

Atherosclerosis is a common cardiovascular disease caused by the abnormal expression of multiple factors and genes influenced by both environmental and genetic factors. The primary manifestation of atherosclerosis is plaque formation, which occurs when inflammatory cells consume excess lipids, affecting their retention and modification within the arterial intima. This triggers endothelial cell (EC) activation, immune cell infiltration, vascular smooth muscle cell (VSMC) proliferation and migration, foam cell formation, lipid streaks, and fibrous plaque development. These processes can lead to vascular wall sclerosis, lumen stenosis, and thrombosis. Immune cells, ECs, and VSMCs in atherosclerotic plaques undergo significant metabolic changes and inflammatory responses. The interaction of cytokines and chemokines secreted by these cells leads to the onset, progression, and regression of atherosclerosis. The regulation of cell- or cytokine-based immune responses is a novel therapeutic approach for atherosclerosis. Statins are currently the primary pharmacological agents utilised for managing unstable plaques owing to their ability to enhance endothelial function, regulate VSMC proliferation and apoptosis by reducing cholesterol levels, and mitigate the expression and activity of inflammatory cytokines. In this review, we provide an overview of the metabolic changes associated with atherosclerosis, describe the effects of inflammatory responses on atherosclerotic plaques, and discuss the mechanisms through which statins contribute to plaque stabilisation. Additionally, we examine the role of statins in combination with other drugs in the management of atherosclerosis.
The analysis of serum lipids profile in Guillain-Barre syndrome
Authors: Wang Lijuan , Ding Yaowei , Liu Jie , Zheng Guanghui , Li Siwen , Jiang Wencan , Chen Kelin , Luan Xin , Chen Yuxin , Wang Siqi , Zhang Guojun
Published: December 2023

Background

Guillain-Barre syndrome (GBS) is an immune-mediated inflammatory peripheral neuropathy. This study aimed to conduct a systematic analysis of the serum lipids profile in GBS.

Methods

We measured the serum lipids profile in 85 GBS patients and compared it with that of 85 healthy controls matched for age and sex. Additionally, we analyzed the correlation between lipids and the severity, subtypes, precursor infections, clinical outcomes, clinical symptoms, immunotherapy, and other laboratory markers of GBS.

Results

Compared to the healthy controls, GBS exhibited significantly elevated levels of Apolipoprotein B (APOB), Apolipoprotein C2 (APOC2), Apolipoprotein C3 (APOC3), Apolipoprotein E (APOE), triglycerides (TG), and residual cholesterol (RC). Conversely, Apolipoprotein A1 (APOA1), Apolipoprotein A2 (APOA2), and high-density lipoprotein (HDL) were substantially lower in GBS. Severe GBS displayed noticeably higher levels of APOC3 and total cholesterol (TC) compared to those with mild disease. Regarding different clinical outcomes, readmitted GBS demonstrated higher RC expression than those who were not readmitted. Moreover, GBS who tested positive for neuro-virus antibody IGG in cerebrospinal fluid (CSF) exhibited heightened expression of APOC3 in comparison to those who tested negative. GBS with cranial nerve damage showed significantly reduced expression of HDL and APOA1 than those without such damage. Additionally, GBS experiencing limb pain demonstrated markedly decreased HDL expression. Patients showed a significant reduction in TC after intravenous immunoglobulin therapy. We observed a significant positive correlation between lipids and inflammatory markers, including TNF-α, IL-1β, erythrocyte sedimentation rate (ESR), white blood cells, monocytes, and neutrophils in GBS. Notably, APOA1 exhibited a negative correlation with ESR. Furthermore, our findings suggest a potential association between lipids and the immune status of GBS.

Conclusion

The research demonstrated a strong connection between lipids and the severity, subtypes, clinical outcomes, precursor infections, clinical symptoms, immunotherapy, inflammation, and immune status of GBS. This implies that a low-fat diet or the use of lipid-lowering medications may potentially serve as an approach for managing GBS, offering a fresh viewpoint for clinical treatment of this condition.
Extracellular vesicles from adipose stem cells ameliorate allergic rhinitis in mice by immunomodulatory
Authors: Yang Wenhan , Pan Zhiyu , Zhang Jiacheng , Wang Lian , lai Ju , Zhou Shican , Zhang Zhili , Fan Kai , Deng Dan , Gao Zhengliang , Yu Shaoqing
Published: December 2023

Background

Human adipose tissue-derived stem cells (hADSCs) exert potent immunosuppressive effects in the allogeneic transplantation treatment. In mouse model of allergic rhinitis (AR), ADSCs partially ameliorated AR. However, no study has evaluated the potential therapeutic effects of hADSC-derived extracellular vesicles (hADSC-EVs) on AR.

Methods

Female BALB/c mice were sensitized and challenged with ovalbumin (OVA) to induce AR. One day after the last nasal drop, each group received phosphate buffered saline (PBS) or hADSC-EVs treatment. Associated symptoms and biological changes were then assessed.

Results

hADSC-EV treatment significantly alleviated nasal symptoms, and reduced inflammatory infiltration. Serum levels of OVA-specific IgE, interleukin (IL)-4 and interferon (IFN)-γ were all significantly reduced. The mRNA levels of IL-4 and IFN-γ in the spleen also changed accordingly. The T helper (Th)1/Th2 cell ratio increased. The treatment efficacy index of hADSC-EV was higher than that of all human-derived MSCs in published reports on MSC treatment of AR. ADSC-EVs exhibited a greater therapeutic index in most measures when compared to our previous treatment involving ADSCs.

Conclusion

These results demonstrated that hADSC-EVs could ameliorate the symptoms of AR by modulating cytokine secretion and Th1/Th2 cell balance. hADSC-EVs could potentially be a viable therapeutic strategy for AR. Further animal studies are needed to elucidate the underlying mechanisms and to optimize potential clinical protocols.
Novel S2 subunit-specific antibody with broad neutralizing activity against SARS-CoV-2 variants of concern
Authors: Heo Chang-Kyu , Lim Won-Hee , Yang Jihyun , Son Sumin , Kim Sang Jick , Kim Doo-Jin , Poo Haryoung , Cho Eun-Wie
Published: December 2023

Introduction

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), had a major impact on both the global health and economy. Numerous virus-neutralizing antibodies were developed against the S1 subunit of SARS-CoV-2 spike (S) protein to block viral binding to host cells and were authorized for control of the COVID-19 pandemic. However, frequent mutations in the S1 subunit of SARS-CoV-2 enabled the emergence of immune evasive variants. To address these challenges, broadly neutralizing antibodies targeting the relatively conserved S2 subunit and its epitopes have been investigated as antibody therapeutics and universal vaccines.

Methods

We initiated this study by immunizing BALB/c mice with β-propiolactone-inactivated SARS-CoV-2 (IAV) to generate B-cell hybridomas. These hybridomas were subsequently screened using HEK293T cells expressing the S2-ECD domain. Hybridomas that produced anti-S2 antibodies were selected, and we conducted a comprehensive evaluation of the potential of these anti-S2 antibodies as antiviral agents and versatile tools for research and diagnostics.

Results

In this study, we present a novel S2-specific antibody, 4A5, isolated from BALB/c mice immunized with inactivated SARS-CoV-2. 4A5 exhibited specific affinity to SARS-CoV-2 S2 subunits compared with those of other β-CoVs. 4A5 bound to epitope segment F1109–V1133 between the heptad-repeat1 (HR1) and the stem-helix (SH) region. The 4A5 epitope is highly conserved in SARS-CoV-2 variants, with a significant conformational feature in both pre- and postfusion S proteins. Notably, 4A5 exhibited broad neutralizing activity against variants and triggered Fc-enhanced antibody-dependent cellular phagocytosis.

Discussion

These findings offer a promising avenue for novel antibody therapeutics and insights for next-generation vaccine design. The identification of 4A5, with its unique binding properties and broad neutralizing capacity, offers a potential solution to the challenge posed by SARS-CoV-2 variants and highlights the importance of targeting the conserved S2 subunit in combating the COVID-19.
Human IgM–expressing memory B cells
Authors: Budeus Bettina , Kibler Artur , Küppers Ralf
Published: December 2023

A hallmark of T cell dependent (TD) humoral immune responses is the generation of long–lived memory B cells. The generation of these cells occurs primarily in the germinal center (GC) reaction, where antigen–activated B cells undergo affinity maturation as a major consequence of the combined processes of proliferation, somatic hypermutation of their immunoglobulin V (IgV) region genes, and selection for improved affinity of their B–cell antigen receptors. As many B cells also undergo class–switching to IgG or IgA in these TD responses, there was traditionally a focus on class–switched memory B cells in both murine and human studies on memory B cells. However, it has become clear that there is also a large subset of IgM–expressing memory B cells, which have important phenotypic and functional similarities but also differences to class–switched memory B cells. There is an ongoing discussion about the origin of distinct subsets of human IgM+ B cells with somatically mutated IgV genes. We argue here that the vast majority of human IgM–expressing B cells with somatically mutated IgV genes in adults is indeed derived from GC reactions, even though a generation of some mostly lowly mutated IgM+ B cells from other differentiation pathways, mainly in early life, may exist.
Forty years of ocean acidification observations (1983–2023) in the Sargasso Sea at the Bermuda Atlantic Time-series Study site
Authors: Bates Nicholas R. , Johnson Rodney J.
Published: December 2023

Ocean physical and biogeochemical conditions are rapidly changing over time. Forty years of observations from 1983 to 2023 collected at the Bermuda Atlantic Time-series Study (BATS) site near Bermuda in the North Atlantic Ocean shows continuing trends of surface warming, increase in salinity, loss of dissolved oxygen (DO), increase in carbon dioxide (CO2), and ocean acidification (OA) effects. Over this period, the ocean has warmed by about +1°C, increased in salinity by +0.136, and lost DO by 12.5 µmol kg−1 or ~6%. Since the 1980s, ocean dissolved inorganic carbon (DIC), total alkalinity (TA), a tracer of anthropogenic CO2 (CTrOCA), and fugacities/partial pressures of CO2 (i.e., fCO2 and pCO2) have continued to increase substantially, with no evidence of a reduction in the rates of change over time. Contemporaneously, ocean pH has decreased by ~0.1 pH units [with ocean acidity (i.e., H+) increasing by >30%], and the saturation states of calcium carbonate minerals (Ωcalcite and Ωaragonite) have decreased. These OA indicators show that the chemical conditions for calcification have become less favorable over the past 40 years. Updating of data and trends at the BATS site show how ocean chemistry of the 2020s is now outside the range observed in the 1980s, and how essential these data are for predicting the response of ocean chemistry and marine ecosystems to future shifting earth and ocean conditions.
Investigating the impact of human blood metabolites on the Sepsis development and progression: a study utilizing two-sample Mendelian randomization
Authors: Zhang Zhongqi , Yin Yu , Chen Tingzhen , You Jinjin , Zhang Wenhui , Zhao Yifan , Ren Yankang , Wang Han , Chen Xiangding , Zuo Xiangrong
Published: December 2023

Background

Existing data suggests a potential link between human blood metabolites and sepsis, yet the precise cause-and-effect relationship remains elusive. By using a two-sample Mendelian randomization (MR) analysis, this study aims to establish a causal link between human blood metabolites and sepsis.

Methods

A two-sample MR analysis was employed to investigate the relationship between blood metabolites and sepsis. To assess the causal connection between sepsis and human blood metabolites, five different MR methods were employed, A variety of sensitivity analyses were conducted, including Cochrane’s Q test, MR-Egger intercept test, MR-PRESSO and leave-one-out (LOO) analysis. In order to ensure the robustness of the causal association between exposure and outcome, the Bonferroni adjustment was employed. Additionally, we conducted analyses of the metabolic pathways of the identified metabolites using the Kyoto Encyclopedia of Genes and Genomes (KEGG) and the Small Molecule Pathway Database (SMPDB) database.

Results

The MR analysis revealed a total of 27 metabolites (16 known and 11 unknown) causally linked to the development and progression of sepsis. After applying the Bonferroni correction, 3-carboxy-4-methyl-5-propyl-2-furanpropanoate (CMPF) remained significant in relation to 28-day all-cause mortality in sepsis. By pathway enrichment analysis, we identified four significant metabolic pathways. Notably, the Alpha Linolenic Acid and Linoleic Acid metabolism pathway emerged as a pivotal contributor to the occurrence and progression of sepsis.

Conclusion

This study provides preliminary evidence of causal associations between human blood metabolites and sepsis, as ascertained by MR analysis. The findings offer valuable insights into the pathogenesis of sepsis and may provide insight into preventive and therapeutic approaches.
Age-dependent dendrobine biosynthesis in Dendrobium nobile: insights into endophytic fungal interactions
Authors: Zhao Yongxia , Ji Xiaolong , Liu Xiaoqi , Qin Lin , Tan Daopeng , Wu Di , Bai Chaojun , Yang Jiyong , Xie Jian , He Yuqi
Published: December 2023

Introduction

Dendrobium nobile (D. nobile), a valued Chinese herb known for its diverse pharmacological effects, owes much of its potency to the bioactive compound dendrobine. However, dendrobine content varies significantly with plant age, and the mechanisms governing this variation remain unclear. This study delves into the potential role of endophytic fungi in shaping host-microbe interactions and influencing plant metabolism.

Methods

Using RNA-seq, we examined the transcriptomes of 1-year-old, 2-year-old, and 3-year-old D. nobile samples and through a comprehensive analysis of endophytic fungal communities and host gene expression in D. nobile stems of varying ages, we aim to identify associations between specific fungal taxa and host genes.

Results

The results revealing 192 differentially expressed host genes. These genes exhibited a gradual decrease in expression levels as the plants aged, mirroring dendrobine content changes. They were enriched in 32 biological pathways, including phagosome, fatty acid degradation, alpha-linolenic acid metabolism, and plant hormone signal transduction. Furthermore, a significant shift in the composition of the fungal community within D. nobile stems was observed along the age gradient. Olipidium, Hannaella, and Plectospherella dominated in 1-year-old plants, while Strelitziana and Trichomerium prevailed in 2-year-old plants. Conversely, 3-year-old plants exhibited additional enrichment of endophytic fungi, including the genus Rhizopus. Two gene expression modules (mediumpurple3 and darkorange) correlated significantly with dominant endophytic fungi abundance and dendrobine accumulation. Key genes involved in dendrobine synthesis were found associated with plant hormone synthesis.

Discussion

This study suggests that the interplay between different endophytic fungi and the hormone signaling system in D. nobile likely regulates dendrobine biosynthesis, with specific endophytes potentially triggering hormone signaling cascades that ultimately influence dendrobine synthesis.
Screening and characterization of biocontrol bacteria isolated from Ageratum conyzoides against Collectotrichum fructicola causing Chinese plum (Prunus salicina Lindl.) anthracnose
Authors: Chen Xiaolin , Zhang Miaomiao , Tang Lihua , Huang Suiping , Guo Tangxun , Li Qili
Published: December 2023

Chinese plum (Prunus salicina Lindl.) is a nutritionally and economically important stone fruit widely grown around the world. Anthracnose, caused by Collectotrichum spp., is one of the primary biotic stress factors limiting plum production. Medicinal plants may harbor rhizospheric or endophytic microorganisms that produce bioactive metabolites that can be used as anthracnose biocontrol agents. Here, 27 bacterial isolates from the medicinal plant A. conyzoides with diverse antagonistic activities against C. fructicola were screened. Based on morphological, physiological, biochemical, and molecular characterization, 25 of these isolates belong to different species of genus Bacillus, one to Pseudomonas monsensis, and one more to Microbacterium phyllosphaerae. Eight representative strains showed high biocontrol efficacy against plum anthracnose in a pot experiment. In addition, several Bacillus isolates showed a broad spectrum of inhibitory activity against a variety of fungal phytopathogens. Analysis of the volatile organic compound profile of these eight representative strains revealed a total of 47 compounds, most of which were ketones, while the others included alkanes, alkenes, alcohols, pyrazines, and phenols. Overall, this study confirmed the potential value of eight bacterial isolates for development as anthracnose biocontrol agents.
Arbuscular mycorrhizal fungi increased peanut (Arachis hypogaea L.) yield by changing the rhizosphere microbial community structure in saline-alkali soil
Authors: Kang Jia , Yang Wenlong , Liu Shangwu , Xuan Ning , Shao Yahui , Geng Yun , Afzal Muhammad , Zhang Yingxin , Yue Shousong , Mushtaq Rubina , Chen Gao
Published: December 2023

Arbuscular mycorrhizal fungi (AMF) have demonstrated the potential to enhance the saline-alkali tolerance in plants. Nevertheless, the extent to which AMF can ameliorate the tolerance of salt-sensitive plants to alkaline conditions necessitates further investigation. The current study is primarily centered on elucidating the impact of AMF on the growth of the Huayu22 (H22) when cultivated in saline-alkaline soil. We leveraged DNA of rhizosphere microorganisms extracted from saline-alkali soil subjected to AMF treatment and conducted high-throughput sequencing encompassing 16S rRNA gene and ITS sequencing. Our findings from high-throughput sequencing unveiled Proteobacteria and Bacillus as the prevailing phylum and genus within the bacterial population, respectively. Likewise, the predominant fungal phylum and genus were identified as Ascomycota and Haematonectria. It is noteworthy that the relative abundance of Proteobacteria, Actinobacteria, Chloroflexi, Bacteroidetes, and Ascomycota exhibited significant increments subsequent to AMF inoculation. Our investigation into soil enzyme activity revealed a remarkable surge post-AMF inoculation. Notably, the amounts of pathogen growth inhibitory enzymes and organic carbon degrading enzymes rise, as predicted by the putative roles of microbial communities. In saline-alkali soil, inoculation of AMF did boost the yield of H22. Notable improvements were observed in the weight of both 100 fruits and 100 grains, which increased by 20.02% and 22.30%, respectively. Conclusively, this study not only provides a theoretical framework but also furnishes empirical evidence supporting the utilization of AMF as a viable strategy for augmenting the yield of salt-sensitive plants grown in alkaline conditions.
Effects of monosaccharides including rare sugars on proliferation of Entamoeba histolytica trophozoites in vitro
Authors: Kato Kentaro , Miura Mitsumasa , Tachibana Hiroshi , Tsukamoto Ikuko
Published: December 2023

Entamoeba histolytica is a parasitic protozoan with roles in pathogenicity of intestinal amoebiasis. E. histolytica trophozoites lack functional mitochondria and their energy production depends mostly on glycolysis. D-Glucose has a pivotal role in this process and trophozoites store this sugar as glycogen in glycogen granules. Rare sugars, which are defined as sugars present in nature in limited amounts, are of interest as natural low-calorie sweeteners for improving physical conditions of humans. One such rare sugar, D-allose, can be absorbed by a sodium-dependent glucose cotransporter as a substitute for D-glucose, and some rare sugars are known to inhibit growth of cancer cells, Caenorhabditis elegans and Tritrichomonas foetus. Based on these observations, we examined the effects of rare sugars on growth of E. histolytica trophozoites, together with those of D-galactose and D-fructose. The results indicate that treatment with D-allose or D-psicose (D-allulose) alone inhibits proliferation of E. histolytica trophozoites, but that these sugars enhance proliferation of trophozoites in the presence of D-glucose or D-galactose. The trophozoites could take up D-glucose and D-galactose, but not D-fructose, D-allose or D-psicose. Cell sizes of the trophozoites also differed depending on the culture medium.
Lumbar multifidus characteristics and body composition in university level athletes
Authors: Anstruther Meagan , Rizk Amanda , Frenette Stephane , Fortin Maryse
Published: December 2023

Introduction

Body composition is well known to affect sport performance and previous studies suggested that structural and functional lumbar multifidus (LM) impairments in athletes were associated with low back pain and lower leg injuries. However few studies have examined the relationship between LM characteristics and body composition in athletic populations.

Methods

This cross-sectional study included a total of 134 university varsity athletes (hockey, soccer, rugby, and football players). Ultrasound imaging was used to examined LM characteristics at the L5 bilaterally [e.g., size, thickness at rest, thickness during contraction, echo-intensity (EI) and % thickness change from rest to contraction] and body composition parameters (dual-energy x-ray absorptiometry). Pearson correlations were used to assess the relationship between LM characteristics and body composition parameters. One-way ANOVA assessed differences in LM characteristics and body composition between sports. All analyses were performed separately by sex.

Results

LM size and thickness were positively correlated with weight, height, lean body mass and total bone mass (male: r = 0.23–0.55, p < 0.01–0.05; female: r = 0.30–0.39, p < 0.01–0.05). LM EI was strongly correlated with % body fat (male: r = 0.62, female: r = 0.71, p < 0.01). LM thickness at rest (r = 0.42, p < 0.01) and contracted (r = 0.27, p < 0.05) were positively correlated and % thickness change was negatively correlated with % body fat in male athletes (r = −0.43, p < 0.01).

Discussion

The significant differences in body composition and LM characteristics between sports may be attributed to sport specific demands. Understanding connections between body composition and LM may aid in preseason screening for athletes at risk of low back pain or lower leg injuries during the season.
Presynaptic GABAB receptors inhibit vomeronasal nerve transmission to accessory olfactory bulb mitral cells
Authors: Weiss Jan , Zufall Frank
Published: December 2023

Vomeronasal sensory neurons (VSNs) recognize pheromonal and kairomonal semiochemicals in the lumen of the vomeronasal organ. VSNs send their axons along the vomeronasal nerve (VN) into multiple glomeruli of the accessory olfactory bulb (AOB) and form glutamatergic synapses with apical dendrites of mitral cells, the projection neurons of the AOB. Juxtaglomerular interneurons release the inhibitory neurotransmitter γ-aminobutyric acid (GABA). Besides ionotropic GABA receptors, the metabotropic GABAB receptor has been shown to modulate synaptic transmission in the main olfactory system. Here we show that GABAB receptors are expressed in the AOB and are primarily located at VN terminals. Electrical stimulation of the VN provokes calcium elevations in VSN nerve terminals, and activation of GABAB receptors by the agonist baclofen abolishes calcium influx in AOB slice preparations. Patch clamp recordings reveal that synaptic transmission from the VN to mitral cells can be completely suppressed by activation of GABAB receptors. A potent GABAB receptor antagonist, CGP 52432, reversed the baclofen-induced effects. These results indicate that modulation of VSNs via activation of GABAB receptors affects calcium influx and glutamate release at presynaptic terminals and likely balances synaptic transmission at the first synapse of the accessory olfactory system.