bluuebunny/crossref_metadata_2025 huggingface dataset:
Application of Ground-Penetrating Radar and a Combined Penetrometer–Moisture Probe for Evaluating Spatial Distribution of Soil Moisture and Soil Hardness in Coastal and Inland Windbreaks
Authors: Iwasaki Kenta , Tamura Makoto , Sato Hirokazu , Masaka Kazuhiko , Oka Daisuke , Yamakawa Yosuke , Kosugi Ken’ichirou
Published: June 2020

The development of a method to easily investigate the spatial distribution of soil moisture and soil hardness in tree windbreaks is necessary because these windbreaks often decline due to inappropriate soil moisture condition and soil compaction. This research examined the applicability of ground-penetrating radar (GPR) and a combined penetrometer–moisture probe (CPMP) for evaluating the spatial distribution of soil moisture and soil hardness in four windbreaks with different soil characteristics. A GPR-reflecting interface was observed at a less permeable layer in a coastal windbreak and at a depth affected by soil compaction in an inland windbreak with andosol. The spatial distribution of the groundwater table could also be evaluated by examining the attenuation of GPR reflection in a coastal windbreak. In contrast, GPR was not applicable in an inland windbreak with peat because of high soil water content near the soil surface. The CPMP could detect vertical distributions of soil hardness and soil water content regardless of soil type. The CPMP was useful for interpreting GPR profiles, and GPR was useful for interpolating the information about the horizontal distribution of soil moisture and soil hardness between survey points made with the CPMP. Thus, the combination of GPR and a CPMP is ideal for examining the two-dimensional spatial distribution of soil moisture and soil hardness at windbreaks with soils for which both methods are applicable.
Prevalence and Determinants of Self-Reported Injuries among Community-Dwelling Older Adults in the Philippines: A 10-Year Pooled Analysis
Authors: Moncatar TJ Robinson , Nakamura Keiko , Siongco Kathryn Lizbeth , Rahman Mosiur , Seino Kaoruko
Published: June 2020

Injury among older adults is a serious health concern, but little information is known about it, particularly in developing countries. This study aimed to determine the prevalence of, and examine the socioeconomic, demographic, and health determinants of, self-reported injuries among older Filipinos. Using a pooled data of 21,316 community-dwelling residents aged 60 years or over from three waves of the Philippine National Demographic and Health Survey, multivariate logistic regression analyses were performed to assess the relationship between participants’ characteristics and reports of injuries. The total prevalence of self-reported injuries over a 10-year period was at 1.2%. Older adults with either government or private health insurance were more likely to report experiencing injuries (adjusted odds ratio (AOR) 1.55, 95% confidence interval (CI), 1.14–2.11), regardless of socio-demographic and economic status. In contrast, female older adults were found to be associated with a lower likelihood of self-reported injuries, after adjustment for other variables (AOR 0.69, 95% CI 0.53–0.88). Older adults who attained secondary education or higher also showed a lower likelihood of self-reported injuries (AOR 0.53, 95% CI 0.31–0.92). The proportion of older adults with injuries in the Philippines is still relatively low. However, preventive approaches with a special focus on gender, educational attainment, and health insurance status of older adults are warranted.
Giving Voice to the Environment as the Silent Partner in Aging: Examining the Moderating Roles of Gender and Family Structure in Older Adult Wellbeing
Authors: Isaacson Michal , Tripathi Ashwin , Samanta Tannistha , D’Ambrosio Lisa , Coughlin Joseph
Published: June 2020

Gerontological scholarship has long seen the environment to be a silent partner in aging. Environmental Gerontology, an established approach in Social Gerontology, has shown how the everyday lives of older adults are deeply entangled in socio-spatial environments. Adopting an Environmental Gerontology approach, we explore social and cultural dimensions of the association between out-of-home mobility and wellbeing among older adults in a north western city of India. This was established by combining high resolution time-space data collected using GPS receivers, questionnaire data and time diaries. Following a multi-staged analytical strategy, we first examine the correlation between out-of-home mobility and wellbeing using bivariate correlation. Second, we introduce gender and family structure into regression models as moderating variables to improve the models’ explanatory power. Finally, we use our results to reinterpret the Ecological Press Model of Aging to include familial structure as a factor that moderates environmental stress. Findings emphasize the central role that social constructs play in the long-established relationship between the environment and the wellbeing of older adults.
Assessing Implementation of Maternal and Perinatal Death Surveillance and Response in Rwanda
Authors: Tayebwa Edwin , Sayinzoga Felix , Umunyana Jacqueline , Thapa Kusum , Ajayi Efugbaike , Kim Young-Mi , van Dillen Jeroen , Stekelenburg Jelle
Published: June 2020

Maternal deaths remain a major public health concern in low- and middle-income countries. Implementation of maternal and perinatal deaths surveillance and response (MPDSR) is vital to reduce preventable deaths. The study aimed to assess implementation of MPDSR in Rwanda. We applied mixed methods following the six-step audit cycle for MPDSR to determine the level of implementation at 10 hospitals and three health centers. Results showed various stages of implementation of MPDSR across facilities. Maternal death audits were conducted regularly, and facilities had action plans to address modifiable factors. However, perinatal death audits were not formally done. Implementation was challenged by lack of enough motivated staff, heavy workload, lack of community engagement, no linkages with existing quality improvement efforts, no guidelines for review of stillbirths, incomplete medical records, poor classification of cause of death, and no sharing of feedback among others. Implementation of MPDSR varied from facility to facility indicating varying capacity gaps. There is need to integrate perinatal death audits with maternal death audits and ensure the process is part of other quality improvement initiatives at the facility level. More efforts are needed to support health facilities to improve implementation of MPDSR and contribute to achieving sustainable development goal (SDG) 3.
Potential Years of Life Lost Due to COVID-19 in the United States, Italy, and Germany: An Old Formula with Newer Ideas
Authors: Mitra Amal K. , Payton Marinelle , Kabir Nusrat , Whitehead April , Ragland Kimberly N. , Brown Alexis
Published: June 2020

Today, the world is facing the challenge of a major pandemic due to COVID-19, which has caused more than 6.1 million cases of infection and nearly 370,000 deaths so far. Most of the deaths from the disease are clustered in the older population, but the young and children are not spared. In this context, there is a critical need to revisit the formula for calculating potential years of life lost (PYLL). Data on age-specific deaths due to COVID-19 in three countries, including the United States (US), Italy, and Germany, were evaluated. New York State, as a significant outlier within the US, was also included. PYLLs in the US were five times as high as those of Italy. Compared with Germany, PYLLs in Italy were 4 times higher, and the rates in the US were 23, 25, and 18 times higher when using upper age limits of 70, 75, and 80, respectively. Standardized PYLLs in New York were 2 times as high as the rates in Italy, and 7 to 9 times as high as PYLLs in Germany. The revised formula of PYLL, using an upper limit of age 80, is recommended to accurately measure premature deaths due to a major disastrous disease such as COVID-19.
Health-Related Behaviors of Pregnant Women Residing in Urban and Rural Areas in Poland
Authors: Szubert Maria , Ilowiecka Malwina , Wilczynski Jacek , Bilinski Przemyslaw , Wojtyla Cezary
Published: June 2020

The aim of this study was to evaluate the knowledge regarding a healthy lifestyle and prophylaxis during pregnancy among women from rural and urban areas and how this changed within a 5-year period. Analyses of the population of pregnant women in Poland were made using a questionnaire survey. The survey was conducted in the years 2010–2012 and 2017. Questionnaires from 6128 pregnant women were collected. The statistical analyses were conducted using IBM SPSS. The examined population was comprised of 41% women from rural areas and 59% women from urban areas. Alcohol consumption was lower among women from rural areas than among urban inhabitants in 2010–2012; in 2017 a trend of even lower consumption was observed. Folic acid supplementation was more broadly developed in the urban population; however, in 2017, higher percentage rates of both populations admitted taking folates before pregnancy. More women in urban than in rural areas performed physical activity during pregnancy, but the differences decreased in 2017. Knowledge of a healthy lifestyle and prophylaxis during pregnancy increased regardless of place of residence; however, the most evident change could be observed among women from rural areas.
Analyzing Service Quality Evaluation Indexes of Rural Last Mile Delivery Using FCE and ISM Approach
Authors: Jiang Xiaohong , Wang Huiying , Guo Xiucheng
Published: June 2020

The advent of e-commerce has led to a rapid acceleration of rural logistics development in China. To enhance green and sustainable development of rural logistics, it is necessary to improve the service quality of the rural last mile delivery and analyze service quality evaluation indexes. An integrated methodology combing fuzzy comprehensive evaluation (FCE) and the interpretative structural model (ISM) is presented in the current paper to reveal the relationship between the service quality evaluation indexes of the rural last mile delivery. A total of 18 logistics service quality evaluation indexes in five dimensions are selected. The FCE is used to measure the service quality of rural delivery in an empirical research area, and the weight of each evaluation index is assigned by regression analysis. The ISM is adopted to judge the hierarchical structure of indexes, and a five-layer hierarchy is obtained. The results show that it is necessary to first focus on improving the evaluation indexes of accuracy of goods arrival and timely customer service response. In the case of Shunfeng Express, the company needs to additionally improve the timeliness and rationality of damaged or lost processing goods. Some countermeasures and suggestions are put forward. The proposed integrated method helps to reveal the key service quality evaluation indexes and the areas needing improvement. The use of regression analysis within the FCE method allows the estimation of weights in a relatively objective way. This research provides theoretical support for improving the service quality and customers’ satisfaction of the rural last mile delivery, and enhancing the green and sustainable development of rural logistics.
Acute Toxicity and Sublethal Effects of Lemongrass Essential Oil and Their Components against the Granary Weevil, Sitophilus granarius
Authors: Plata-Rueda Angelica , Rolim Gabriela Da Silva , Wilcken Carlos Frederico , Zanuncio José Cola , Serrão José Eduardo , Martínez Luis Carlos
Published: June 2020

In the present work, we evaluate the toxic and repellent properties of lemongrass (Cymbopogon citratus (DC. ex Nees) Stapf.) essential oil and its components against Sitophilus granarius Linnaeus as an alternative to insecticide use. The lethal dose (LD50 and LD90), survivorship, respiration rate, and repellency on adults of S. granarius exposed to different doses of lemongrass oil and some of its components were evaluated. The chemical composition of the essential oil was found to have the major components of neral (24.6%), citral (18.7%), geranyl acetate (12.4%), geranial (12.3%), and limonene (7.55%). Lemongrass essential oil (LD50 = 4.03 µg·insect–1), citral (LD50 = 6.92 µg·insect–1), and geranyl acetate (LD50 = 3.93 µg·insect–1) were toxic to S. granarius adults. Survivorship was 99.9% in insects not exposed to lemongrass essential oil, decreasing to 57.6%, 43.1%, and 25.9% in insects exposed to LD50 of essential oil, citral, and geranyl acetate, respectively. The insects had low respiratory rates and locomotion after exposure to the essential oil, geranyl acetate, and citral. Our data show that lemongrass essential oils and their components have insecticidal and repellent activity against S. granarius and, therefore, have the potential for application in stored grain pest management schemes.
Slug Monitoring and Impacts on the Ground Beetle Community in the Frame of Sustainable Pest Control in Conventional and Conservation Agroecosystems
Authors: Scaccini Davide , Panini Michela , Chiesa Olga , Nicoli Aldini Rinaldo , Tabaglio Vincenzo , Mazzoni Emanuele
Published: June 2020

In conservation agriculture, slugs are considered significant pests and their monitoring is a key option in the integrated pest management framework. Together with molluscicide applications, predators such as ground beetles can offer a tool for slug control in the field. Through the evaluation of slug and ground beetle monitoring strategies, this work compared their presence in conventional and conservation agricultural plots. The invasive Deroceras invadens was the dominant slug species to occur in all sampling periods. Among Carabidae, Poecilus cupreus and Pterostichus melas were the most abundant species, and Bembidion spp., Brachinus spp., and Harpalus spp. were also common. Beer-baited pitfall traps, whatever their alcoholic content, caught more slugs and ground beetles than wooden boards used as shelters. Slugs were more abundant in conventional plots than in conservation plots, possibly due to the lower presence of natural enemies such as ground beetles. Despite possible impacts on Carabidae, beer-baited pitfall traps should be considered a useful tool for slug monitoring and for the planning of molluscicide applications. Soil management such as minimum- or no-tillage and the presence of cover crops are important elements influencing both slug and ground beetle presence, possibly playing a key role in the maintenance of natural enemy populations.
Birnbaum-Saunders Quantile Regression Models with Application to Spatial Data
Authors: Sánchez Luis , Leiva Víctor , Galea Manuel , Saulo Helton
Published: June 2020

In the present paper, a novel spatial quantile regression model based on the Birnbaum–Saunders distribution is formulated. This distribution has been widely studied and applied in many fields. To formulate such a spatial model, a parameterization of the multivariate Birnbaum–Saunders distribution, where one of its parameters is associated with the quantile of the respective marginal distribution, is established. The model parameters are estimated by the maximum likelihood method. Finally, a data set is applied for illustrating the formulated model.
Direct Measurement of Crossover and Interfacial Resistance of Ion-Exchange Membranes in All-Vanadium Redox Flow Batteries
Authors: Ashraf Gandomi Yasser , Aaron Doug , Nolan Zachary , Ahmadi Arya , Mench Matthew
Published: June 2020

Among various components commonly used in redox flow batteries (RFBs), the separator plays a significant role, influencing resistance to current as well as capacity decay via unintended crossover. It is well-established that the ohmic overpotential is dominated by the membrane and interfacial resistance in most aqueous RFBs. The ultimate goal of engineering membranes is to improve the ionic conductivity while keeping crossover at a minimum. One of the major issues yet to be addressed is the contribution of interfacial phenomena in the influence of ionic and water transport through the membrane. In this work, we have utilized a novel experimental system capable of measuring the ionic crossover in real-time to quantify the permeability of ionic species. Specifically, we have focused on quantifying the contributions from the interfacial resistance to ionic crossover. The trade-off between the mass and ionic transport impedance caused by the interface of the membranes has been addressed. The MacMullin number has been quantified for a series of electrolyte configurations and a correlation between the ionic conductivity of the contacting electrolyte and the Nafion® membrane has been established. The performance of individual ion-exchange membranes along with a stack of various separators have been explored. We have found that utilizing a stack of membranes is significantly beneficial in reducing the electroactive species crossover in redox flow batteries compared to a single membrane of the same fold thickness. For example, we have demonstrated that the utilization of five layers of Nafion® 211 membrane reduces the crossover by 37% while only increasing the area-specific resistance (ASR) by 15% compared to a single layer Nafion® 115 membrane. Therefore, the influence of interfacial impedance in reducing the vanadium ion crossover is substantially higher compared to a corresponding increase in ASR, indicating that mass and ohmic interfacial resistances are dissimilar. We have expanded our analysis to a combination of commercially available ion-exchange membranes and provided a design chart for membrane selection based on the application of interest (short duration/high-performance vs. long-term durability). The results of this study provide a deeper insight into the optimization of all-vanadium redox flow batteries (VRFBs).
Mechanism of Nickel, Magnesium, and Iron Recovery from Olivine Bearing Ore during Leaching with Hydrochloric Acid Including a Carbonation Pre-Treatment
Authors: Matus Carlos , Stopic Srecko , Etzold Simon , Kremer Dario , Wotruba Hermann , Dertmann Christian , Telle Rainer , Friedrich Bernd , Knops Pol
Published: June 2020

This work continues on from previous studies showing that mineral sequestration by carbonation of magnesium or calcium silicates under high pressure and high temperature can be successfully carried out by processing in an autoclave. The paper is focused on the influence of experimental parameters on avoiding scale formation during pre-treatment in an autoclave and a subsequent leaching. Amorphous silica and magnesite, respectively, were the main reaction products in a carbonation of olivine under high pressure conditions in an autoclave. In addition, the examined peridotites may be accompanied by small to medium amounts of nickel or other metals, the recovery of which will be investigated in the present study: Extraction of metals such as nickel, iron, and magnesium from olivine bearing ore using hydrochloric acid under atmospheric pressure was studied between 50 and 90 °C in 1 h. The obtained results have shown maximal leaching efficiency of about 35% for Ni, Fe, and Mg under atmospheric pressure, in comparison to more than 60% obtained under the same conditions after a carbonation pre-treatment in an autoclave. Silica gel was formed during leaching without a pre-treatment of peridotite blocking the leaching process, which is not the case for the pre-treated material. The influence of temperature, reaction time, particle size and pre-treatment of peridotite in an autoclave during carbonation at 175 °C and 71.5 bar was studied. A new mechanism model for metal extraction from olivine-bearing ore by avoiding silica gel formation during leaching with hydrochloric acid including a carbonation pre-treatment is proposed. This study explains additionally a behavior of metals such as nickel, magnesium, and iron during a carbonation of olivine bearing ore and leaching of a carbonated solid product.
Microstructural and Mechanical Response of NiTi Lattice 3D Structure Produced by Selective Laser Melting
Authors: Biffi Carlo Alberto , Bassani Paola , Fiocchi Jacopo , Tuissi Ausonio
Published: June 2020

Nowadays, additive manufacturing (AM) permits to realize complex metallic structural parts, and the use of NiTi alloy, known as Nitinol, allows the integration of specific functions to the AM products. One of the most promising designs for AM is concerning the use of lattice structures that show lightweight, higher than bulk material deformability, improved damping properties, high exchange surface. Moreover, lattice structures can be realized with struts, having dimensions below 1 mm—this is very attractive for the realization of Nitinol components for biomedical devices. In this light, the present work regarded the experimental characterization of lattice structures, produced by selective laser melting (SLM), by using Ni-rich NiTi alloy. Differential scanning calorimetry (DSC), electron backscatter diffraction (EBSD), and compression testing were carried out for analyzing microstructure, martensitic transformation (MT) evolution, and superelasticity response of the SLMed lattice samples. The lattice microstructures were compared with those of the SLMed bulk material for highlighting differences. Localized martensite was detected in the nodes zones, where the rapid solidification tends to accumulate solidification stresses. An increase of martensitic transformation temperatures was also observed in lattice NiTi.
Bioleaching of Heavy Metals from Municipal Solid Waste Incineration Fly Ash: Availability of Recoverable Sulfur Prills and Form Transformation of Heavy Metals
Authors: Zhang Ruichang , Wei Xuefeng , Hao Qiang , Si Ruofan
Published: June 2020

Bioleaching is an effective and promising approach for the recovery or removal of heavy metals from metal-laden municipal solid waste incineration fly ash. To exclude the risk of reacidification of the leached fly ash after bioleaching with sulfur powder, molded sulfur prills were used as energy substrate for sulfur oxidizing bacteria to examine the availability of reusing the recyclable sulfur forms. The chemical species of heavy metals during the bioleaching process were also investigated. Results showed that the pH reduction, sulfate production, and metal solubilization with sulfur prills were comparable to that with sulfur powder despite of the theoretically calculated smaller surface of the formers. After 15 days of bioleaching, 80.7–82.1% of Cd, 72.5–74.1% of Zn, 42.8–43.9% of Cu, 24.1–25.2% of Cr, and 12.4–13.0% of Pb were removed from the fly ash, respectively. During bioleaching, heavy metals in the acid extractable and reducible fraction were significantly removed, and metals in oxidizable from were partially reduced. The low leaching toxicity of heavy metals according to toxicity characteristic leaching procedure (TCLP) verified the effective detoxification of fly ash. Moreover, the comparable pH reduction and metal removal efficiencies of bioleaching process with recycled sulfur prills to that with fresh sulfur revealed the potential of reusing the recoverable sulfur prills in the bioleaching process for decontamination of heavy metals from municipal solid waste fly ash.
Comparison of Metabolomic Profiles of Organs in Mice of Different Strains Based on SPME-LC-HRMS
Authors: Burlikowska Katarzyna , Stryjak Iga , Bogusiewicz Joanna , Kupcewicz Bogumiła , Jaroch Karol , Bojko Barbara
Published: June 2020

Given that the extent to which genetics alters the metabolomic profile of tissues is still poorly understood, the current study aimed to characterize and investigate the metabolite profiles of brain, liver, kidney and skeletal muscle of two common mouse inbred strains (BALB/c, C57BL/6) and one outbred stock (CD1) for strain-specific differences. Male mice (n = 15) at the age of 12 weeks were used: BALB/c (n = 5), C57BL/6 (n = 5) and CD1 (n = 5). Solid phase microextraction (SPME) was applied for the extraction of analytes from the tissues. SPME fibers (approximately 0.2 mm in diameter) coated with a biocompatible sorbent (4 mm length of hydrophilic-lipophilic balanced particles) were inserted into each organ immediately after euthanasia. Samples were analyzed using liquid chromatography coupled to a Q-Exactive Focus Orbitrap mass spectrometer. Distinct interstrain differences in the metabolomic patterns of brain and liver tissue were revealed. The metabolome of kidney and muscle tissue in BALB/c mice differed greatly from C57BL/6 and CD1 strains. The main compounds differentiating all the targeted organs were alpha-amino acids, purine nucleotides and fatty acid esters. The results of the study indicate that the baseline metabolome of organs, as well as different metabolic pathways, vary widely among general-purpose models of laboratory mice commonly used in biomedical research.
Evolution of Biochip Technology: A Review from Lab-on-a-Chip to Organ-on-a-Chip
Authors: Azizipour Neda , Avazpour Rahi , Rosenzweig Derek H. , Sawan Mohamad , Ajji Abdellah
Published: June 2020

Following the advancements in microfluidics and lab-on-a-chip (LOC) technologies, a novel biomedical application for microfluidic based devices has emerged in recent years and microengineered cell culture platforms have been created. These micro-devices, known as organ-on-a-chip (OOC) platforms mimic the in vivo like microenvironment of living organs and offer more physiologically relevant in vitro models of human organs. Consequently, the concept of OOC has gained great attention from researchers in the field worldwide to offer powerful tools for biomedical researches including disease modeling, drug development, etc. This review highlights the background of biochip development. Herein, we focus on applications of LOC devices as a versatile tool for POC applications. We also review current progress in OOC platforms towards body-on-a-chip, and we provide concluding remarks and future perspectives for OOC platforms for POC applications.
Energy-Efficient Single-Stage Nitrite Shunt Denitrification with Saline Sewage through Concise Dissolved Oxygen (DO) Supply: Process Performance and Microbial Communities
Authors: Zhuang Huichuan , Wu Zhuoying , Xu Linji , Leu Shao-Yuan , Lee Po-Heng
Published: June 2020

Single-stage nitrite shunt denitrification (through nitrite rather than nitrate) with low dissolved oxygen (DO) supply is a better alternative in terms of energy-efficiency, short-footprint, and low C/N-ratio requirement. This study investigates the optimal DO level with temperature effect, with saline sewage at the fixed hydraulic and solids retention times of 8 h and 8 d, respectively. Moreover, 16S rRNA gene sequencing analysis corresponding with total nitrogen (TN) and chemical oxygen demand (COD) removals in each operating condition were performed. Results showed that DO of 0.3 mg/L at 20 °C achieved over 60.7% and over 97.9% of TN and COD removal, respectively, suggesting that such condition achieved effective nitrite-oxidizing bacteria inhibition and efficient denitrification. An unexpected finding was that sulfur-reducing Haematobacter and nitrogen-fixing Geofilum and Shinella were highly abundant with the copredominance of ammonia-oxidizing Comamonas and Nitrosomonas, nitrite-oxidizing Limnohabitans, and denitrifying Simplicispira, Castellaniella, and Nitratireductor. Further, canonical correspondence analysis (CCA) with respect to the operating conditions associated with phenotype prediction via R-based tool Tax4Fun was performed for a preliminary diagnosis of microbial functionality. The effects of DO, temperature, nitrite, and nitrate in various extents toward each predominant microbe were discussed. Collectively, DO is likely pivotal in single-stage nitrite shunt denitrification, as well as microbial communities, for energy-efficient saline sewage treatment.
Pontiella desulfatans gen. nov., sp. nov., and Pontiella sulfatireligans sp. nov., Two Marine Anaerobes of the Pontiellaceae fam. nov. Producing Sulfated Glycosaminoglycan-like Exopolymers
Authors: van Vliet Daan M. , Lin Yuemei , Bale Nicole J. , Koenen Michel , Villanueva Laura , Stams Alfons J. M. , Sánchez-Andrea Irene
Published: June 2020

Recently, we isolated two marine strains, F1T and F21T, which together with Kiritimatiella glycovorans L21-Fru-ABT are the only pure cultures of the class Kiritimatiellae within the phylum Verrucomicrobiota. Here, we present an in-depth genome-guided characterization of both isolates with emphasis on their exopolysaccharide synthesis. The strains only grew fermentatively on simple carbohydrates and sulfated polysaccharides. Strains F1T, F21T and K. glycovorans reduced elemental sulfur, ferric citrate and anthraquinone-2,6-disulfonate during anaerobic growth on sugars. Both strains produced exopolysaccharides during stationary phase, probably with intracellularly stored glycogen as energy and carbon source. Exopolysaccharides included N-sulfated polysaccharides probably containing hexosamines and thus resembling glycosaminoglycans. This implies that the isolates can both degrade and produce sulfated polysaccharides. Both strains encoded an unprecedently high number of glycoside hydrolase genes (422 and 388, respectively), including prevalent alpha-L-fucosidase genes, which may be necessary for degrading complex sulfated polysaccharides such as fucoidan. Strain F21T encoded three putative glycosaminoglycan sulfotransferases and a putative sulfate glycosaminoglycan biosynthesis gene cluster. Based on phylogenetic and chemotaxonomic analyses, we propose the taxa Pontiella desulfatans F1T gen. nov., sp. nov. and Pontiella sulfatireligans F21T sp. nov. as representatives of the Pontiellaceae fam. nov. within the class Kiritimatiellae.
Thermal State, Thickness, and Composition of the Lithospheric Mantle beneath the Upper Muna Kimberlite Field (Siberian Craton) Constrained by Clinopyroxene Xenocrysts and Comparison with Daldyn and Mirny Fields
Authors: Dymshits Anna M. , Sharygin Igor S. , Malkovets Vladimir G. , Yakovlev Igor V. , Gibsher Anastasia A. , Alifirova Taisia A. , Vorobei Sofya S. , Potapov Sergey V. , Garanin Viktor K.
Published: June 2020

To gain better insight into the thermal state and composition of the lithospheric mantle beneath the Upper Muna kimberlite field (Siberian craton), a suite of 323 clinopyroxene xenocrysts and 10 mantle xenoliths from the Komsomolskaya-Magnitnaya (KM) pipe have been studied. We selected 188 clinopyroxene grains suitable for precise pressure (P)-temperature (T) estimation using single-clinopyroxene thermobarometry. The majority of P-T points lie along a narrow, elongated field in P-T space with a cluster of high-T and high-P points above 1300 °C, which deviates from the main P-T trend. The latter points may record a thermal event associated with kimberlite magmatism (a “stepped” or “kinked” geotherm). In order to eliminate these factors, the steady-state mantle paleogeotherm for the KM pipe at the time of initiation of kimberlite magmatism (Late Devonian–Early Carboniferous) was constrained by numerical fitting of P-T points below T = 1200 °C. The obtained mantle paleogeotherm is similar to the one from the nearby Novinka pipe, corresponding to a ~34–35 mW/m2 surface heat flux, 225–230 km lithospheric thickness, and 110–120 thick “diamond window” for the Upper Muna field. Coarse peridotite xenoliths are consistent in their P-T estimates with the steady-state mantle paleogeotherm derived from clinopyroxene xenocrysts, whereas porphyroclastic ones plot within the cluster of high-T and high-P clinopyroxene xenocrysts. Discrimination using Cr2O3 demonstrates that peridotitic clinopyroxene xenocrysts are prevalent (89%) among all studied 323 xenocrysts, suggesting that the Upper Muna mantle is predominantly composed of peridotites. Clinopyroxene-poor or -free peridotitic rocks such as harzburgites and dunites may be evident at depths of 140–180 km in the Upper Muna mantle. Judging solely from the thermal considerations and the thickness of the lithosphere, the KM and Novinka pipes should have excellent diamond potential. However, all pipes in the Upper Muna field have low diamond grades (<0.9, in carats/ton), although the lithosphere thickness is almost similar to the values obtained for the high-grade Udachnaya and Mir pipes from the Daldyn and Mirny fields, respectively. Therefore, other factors have affected the diamond grade of the Upper Muna kimberlite field.
Efficacy, Stability, and Safety Evaluation of New Polyphenolic Xanthones Towards Identification of Bioactive Compounds to Fight Skin Photoaging
Authors: Resende Diana I. S. P. , Almeida Mariana C. , Maciel Bruna , Carmo Helena , Sousa Lobo José , Dal Pozzo Carlotta , Cravo Sara M. , Rosa Gonçalo P. , Kane-Pagès Aida , do Carmo Barreto Maria , Almeida Isabel F , de Sousa Maria Emília , Pinto Madalena M. M.
Published: June 2020

Antioxidants have long been used in the cosmetic industry to prevent skin photoaging, which is mediated by oxidative stress, making the search for new antioxidant compounds highly desirable in this field. Naturally occurring xanthones are polyphenolic compounds that can be found in microorganisms, fungi, lichens, and some higher plants. This class of polyphenols has a privileged scaffold that grants them several biological activities. We have previously identified simple oxygenated xanthones as promising antioxidants and disclosed as hit, 1,2-dihydroxyxanthone (1). Herein, we synthesized and studied the potential of xanthones with different polyoxygenated patterns as skin antiphotoaging ingredients. In the DPPH antioxidant assay, two newly synthesized derivatives showed IC50 values in the same range as ascorbic acid. The synthesized xanthones were discovered to be excellent tyrosinase inhibitors and weak to moderate collagenase and elastase inhibitors but no activity was revealed against hyaluronidase. Their metal-chelating effect (FeCl3 and CuCl2) as well as their stability at different pH values were characterized to understand their potential to be used as future cosmetic active agents. Among the synthesized polyoxygenated xanthones, 1,2-dihydroxyxanthone (1) was reinforced as the most promising, exhibiting a dual ability to protect the skin against UV damage by combining antioxidant/metal-chelating properties with UV-filter capacity and revealed to be more stable in the pH range that is close to the pH of the skin. Lastly, the phototoxicity of 1,2-dihydroxyxanthone (1) was evaluated in a human keratinocyte cell line and no phototoxicity was observed in the concentration range tested.
An Eco-Friendly Hydrophobic Deep Eutectic Solvent-Based Dispersive Liquid–Liquid Microextraction for the Determination of Neonicotinoid Insecticide Residues in Water, Soil and Egg Yolk Samples
Authors: Kachangoon Rawikan , Vichapong Jitlada , Santaladchaiyakit Yanawath , Burakham Rodjana , Srijaranai Supalax
Published: June 2020

A green, simple and sensitive hydrophobic DES-based dispersive liquid–liquid microextraction coupled with high-performance liquid chromatography (HPLC) was developed for the analysis of neonicotinoid insecticide residues in various samples. A hydrophobic deep eutectic solvent (DES) was synthesized using decanoic acid as a hydrogen bond donor and tetrabutylammonium bromide (TBABr) as a hydrogen bond-acceptor. DESs were synthesized and characterized by Fourier transform-infrared (FTIR) spectroscopy. Two disperser solvents were substituted with surfactants and acetonitrile, which could afford more effective emulsification and make the extraction relatively greener. The hydrophobic DES extraction phase occurred 10 min after centrifugation, being easy to be collected for analysis. Several parameters were investigated and optimized. Under the optimum condition, the calibration curve of this method was linear in the range of 0.003–1.0-µg·mL−1, with a correlation coefficient (R2) higher than 0.99 and a good repeatability, with the relative standard deviations (RSDs) were less than 5.00%. The limits of detection were in the range of 0.001–0.003 µg·mL−1; the limits of quantitation were in the range of 0.003–0.009 µg·mL·mL−1. Finally, the presented method was implemented to determine the neonicotinoid insecticide residues in water, soil, egg yolk samples and acceptable recoveries were obtained.
Effects of Rich in Β-Glucans Edible Mushrooms on Aging Gut Microbiota Characteristics: An In Vitro Study
Authors: Mitsou Evdokia K. , Saxami Georgia , Stamoulou Emmanuela , Kerezoudi Evangelia , Terzi Eirini , Koutrotsios Georgios , Bekiaris Georgios , Zervakis Georgios I. , Mountzouris Konstantinos C. , Pletsa Vasiliki , Kyriacou Adamantini
Published: June 2020

Alterations of gut microbiota are evident during the aging process. Prebiotics may restore the gut microbial balance, with β-glucans emerging as prebiotic candidates. This study aimed to investigate the impact of edible mushrooms rich in β-glucans on the gut microbiota composition and metabolites by using in vitro static batch culture fermentations and fecal inocula from elderly donors (n = 8). Pleurotus ostreatus, P. eryngii, Hericium erinaceus and Cyclocybe cylindracea mushrooms derived from various substrates were examined. Gut microbiota composition (quantitative PCR (qPCR)) and short-chain fatty acids (SCFAs; gas chromatography (GC)) were determined during the 24-h fermentation. P. eryngii induced a strong lactogenic effect, while P. ostreatus and C. cylindracea induced a significant bifidogenic effect (p for all <0.05). Furthermore, P. eryngii produced on wheat straw and the prebiotic inulin had comparable Prebiotic Indexes, while P. eryngii produced on wheat straw/grape marc significantly increased the levels of tested butyrate producers. P. ostreatus, P. eryngii and C. cylindracea had similar trends in SCFA profile; H. erinaceus mushrooms were more diverse, especially in the production of propionate, butyrate and branched SCFAs. In conclusion, mushrooms rich in β-glucans may exert beneficial in vitro effects in gut microbiota and/or SCFAs production in elderly subjects.
Changes of Ginsenoside Composition in the Creation of Black Ginseng Leaf
Authors: Chen Wei , Balan Prabhu , Popovich David G.
Published: June 2020

Ginseng is an increasingly popular ingredient in supplements for healthcare products and traditional medicine. Heat-processed ginsengs, such as red ginseng or black ginseng, are regarded as more valuable for medicinal use when compared to white ginseng due to some unique less polar ginsenosides that are produced during heat-treatment. Although ginseng leaf contains abundant ginsenosides, attention has mostly focused on ginseng root; relatively few publications have focused on ginseng leaf. Raw ginseng leaf was steamed nine times to make black ginseng leaf using a process that is similar to that used to produce black ginseng root. Sixteen ginsenosides were analyzed during each steaming while using high-performance liquid chromatography (HPLC). The contents of ginsenosides Rd and Re decreased and the less polar ginsenosides (F2, Rg3, Rk2, Rk3, Rh3, Rh4, and protopanaxatriol) enriched during steam treatment. After nine cycles of steaming, the contents of the less polar ginsenosides F2, Rg3, and Rk2 increased by 12.9-fold, 8.6-fold, and 2.6-fold, respectively. Further, we found that the polar protopanaxadiol (PPD) -type ginsenosides are more likely to be converted from ginsenoside Rg3 to ginsenosides Rk1 and Rg5 via dehydration from Rg3, and from ginsenoside Rh2 to ginsenosides Rk2 and Rh3 through losing an H2O molecule than to be completely degraded to the aglycones PPD during the heat process. This study suggests that ginseng leaves can be used to produce less polar ginsenosides through heat processes, such as steaming.
FAM83G Is a Novel Inducer of Apoptosis
Authors: Okada Junichi , Sunaga Noriaki , Yamada Eijiro , Saito Tsugumichi , Ozawa Atsushi , Nakajima Yasuyo , Okada Kazuya , Pessin Jeffrey E. , Okada Shuichi , Yamada Masanobu
Published: June 2020

The family with sequence similarity 83 (FAM83) protein family G (FAM83G) possesses a predicted consensus phosphorylation motif for serine/threonine-protein kinase D1/protein kinase C mu (PKD1/PKCμ) at serine residue 356 (S356). In this study, overexpressed wild-type FAM83G coimmunoprecipitated with PKD1/PKCμ in Chinese hamster ovary (CHO) cells inhibited heat shock protein 27 (HSP27) phosphorylation at S82 and reduced the living cell number. The expression of a FAM83G phosphorylation-resistant mutant (S356A-FAM83G) had no effect on the living cell number or the induction of spontaneous apoptosis. By contrast, the introduction of a synthetic peptide encompassing FAM83G S356 into HCT116 and HepG2 cells decreased HSP27 S15 and S82 phosphorylation and induced spontaneous apoptosis. On the other hand, the introduction of FAM83G phosphorylation-resistant mutant synthesized peptides (S356A-AF-956 and S356A-AG-066) did not reduce the living cell number or induce spontaneous apoptosis. The endogenous expression of HSP27 and FAM83G was apparently greater in HCT116 and HepG2 cells compared with in CHO cells. In various types of lung cancer cell lines, the FAM83G messenger RNA (mRNA) level in non-small lung cancer cells was at a similar level to that in non-cancerous cells. However, the FAM83G mRNA level in the small cell lung cancer cell lines was variable, and the HSP27 mRNA level in FAM83G mRNA-rich types was greater than that in FAM83G mRNA-normal range types. Taken together, these data demonstrate that FAM83G S356 phosphorylation modulates HSP27 phosphorylation and apoptosis regulation and that HSP27 is a counterpart of FAM83G.
Cytotoxicity Evaluation of Novel bis(2-aminoethyl)amine Derivatives
Authors: Szulczyk Daniel , Bielenica Anna , Roszkowski Piotr , Dobrowolski Michał A. , Olejarz Wioletta , Napiórkowska Mariola , Struga Marta
Published: June 2020

Seven novel derivatives of bis(2-aminoethyl)amine were synthesized. For compounds 1 and 7 single crystals were isolated and X-ray diffraction experiments were done. Lipophilicity and drug likeness were calculated in the initial stage of research. All compounds were screened for their in vitro cytotoxic activity against a panel of human cancer cell lines, which is contrary to normal (HaCaT) cell lines, by using the MTT method. Studies were followed by lactate dehydrogenase assay, apoptotic activity, and interleukin-6 assay. Within the studied group, compound 6 showed the most promising results in all biological studies. The strongest influence in A549 cells was denoted for derivative 4, which inhibited interleukin release almost tenfold, as compared to the control.
Taurine and Ginsenoside Rf Induce BDNF Expression in SH-SY5Y Cells: A Potential Role of BDNF in Corticosterone-Triggered Cellular Damage
Authors: Lee Won Jin , Lee Gyeong Hee , Hur Jinwoo , Lee Hyuk Gyoon , Kim Eunsu , Won Jun Pil , Cho Youngjae , Choi Mi-Jung , Seo Han Geuk
Published: June 2020

This study shows that taurine and ginsenoside Rf act synergistically to increase the expression of brain-derived neurotrophic factor (BDNF) in SH-SY5Y human neuroblastoma cells in a dose- and time-dependent manner. The increase of BDNF mRNA by taurine and ginsenoside Rf was markedly attenuated by inhibitors of extracellular signal-regulated kinase and p38 mitogen-activated protein kinase. In addition, taurine and ginsenoside Rf protected cells from corticosterone-induced BDNF suppression and reduced cell viability and lactate dehydrogenase release. The results from this study showed that combined treatment with both taurine and ginsenoside Rf enhanced BDNF expression and protected cells against corticosterone-induced damage.
Empowering the Medicinal Applications of Bisphosphonates by Unveiling their Synthesis Details
Authors: Barbosa Jéssica S. , Braga Susana Santos , Almeida Paz Filipe A.
Published: June 2020

Bisphosphonates (BPs), well-known medicinal compounds used for osteoporosis management, are currently the target of intensive research, from basic pre-formulation studies to more advanced stages of clinical practice. The high demand by the pharmaceutical industry inherently requires an easy, efficient and quick preparation of BPs. Current synthetic procedures are, however, still far from ideal. This work presents a comprehensive compilation of reports on the synthesis of the commercially available bisphosphonates that are pharmaceutical active ingredients. Current limitations to the conventional synthesis are assessed, and paths towards their improvement are described, either through the use of alternative solvents and/or by selecting appropriate ratios of the reactants. Innovative processes, such as microwave-assisted synthesis, are presented as more environmental-friendly and effective methods. The main advantages and setbacks of all syntheses are provided as a way to clarify and promote the development of simpler and improved procedures. Only in this way one will be able to efficiently respond to the future high demand of BPs, mostly due to the increase in life span in occidental countries.
Large-Area Fabrication of Laser-Induced Periodic Surface Structures on Fused Silica Using Thin Gold Layers
Authors: Kunz Clemens , Engel Sebastian , Müller Frank , Gräf Stephan
Published: June 2020

Despite intensive research activities in the field of laser-induced periodic surface structures (LIPSS), the large-area nanostructuring of glasses is still a challenging problem, which is mainly caused by the strongly non-linear absorption of the laser radiation by the dielectric material. Therefore, most investigations are limited to single-spot experiments on different types of glasses. Here, we report the homogeneous generation of LIPSS on large-area surfaces of fused silica using thin gold layers and a fs-laser with a wavelength λ = 1025 nm, a pulse duration τ = 300 fs, and a repetition frequency frep = 100 kHz as radiation source. For this purpose, single-spot experiments are performed to study the LIPSS formation process as a function of laser parameters and gold layer thickness. Based on these results, the generation of large-area homogenous LIPSS pattern was investigated by unidirectional scanning of the fs-laser beam across the sample surface using different line spacing. The nanostructures are characterized by a spatial period of about 360 nm and a modulation depth of around 160 nm. Chemical surface analysis by Raman spectroscopy confirms a complete ablation of the gold film by the fs-laser irradiation. The characterization of the functional properties shows an increased transmission of the nanostructured samples accompanied by a noticeable change in the wetting properties, which can be additionally modified within a wide range by silanization. The presented approach enables the reproducible LIPSS-based laser direct-writing of sub-wavelength nanostructures on glasses and thus provides a versatile and flexible tool for novel applications in the fields of optics, microfluidics, and biomaterials.
Graphene Oxide-Polypyrrole Coating for Functional Ceramics
Authors: Rosas-Laverde Nelly Ma. , Pruna Alina Iuliana , Busquets-Mataix David
Published: June 2020

Ceramic substrates were metallized with a Ni-Mo-P electroless coating and further modified with a polypyrrole (PPy) coating by the electrodeposition method. The properties of the polypyrrole coating were studied with the addition of a graphene oxide (GO) nanomaterial prior to the electrodeposition and its reduction degree. Fourier Transform Infrared Spectroscopy, Field-Emission Scanning Electron Microscopy, Raman spectroscopy and cyclic voltammetry were employed to characterize the properties of the coatings. The results indicated the successful synthesis of conductive electrodes by the proposed approach. The electrodeposition of PPy and its charge storage properties are improved by chemically reduced GO. The surface capacitive contribution to the total stored charge was found to be dominant and increased 2–3 fold with the reduction of GO. The chemically reduced GO-modified PPy exhibits the highest capacitance of 660 F g−1 at 2 mV s−1, and shows a good cyclability of 94% after 500 charge/discharge cycles. The enclosed results indicate the use of an NiMoP electroless coating, and modification with a carbon nanomaterial and conducting polymer is a viable approach for achieving functional ceramics.