bluuebunny/crossref_metadata_2025 huggingface dataset:
RETRACTED: Green Synthesis of Metallic Nanoparticles and Their Potential Applications to Treat Cancer
Authors: Zhang Dan , Ma Xin-lei , Gu Yan , Huang He , Zhang Guang-wei
Published: October 2020

Nanoparticle synthesis using microorganisms and plants by green synthesis technology is biologically safe, cost-effective, and environment-friendly. Plants and microorganisms have established the power to devour and accumulate inorganic metal ions from their neighboring niche. The biological entities are known to synthesize nanoparticles both extra and intracellularly. The capability of a living system to utilize its intrinsic organic chemistry processes in remodeling inorganic metal ions into nanoparticles has opened up an undiscovered area of biochemical analysis. Nanotechnology in conjunction with biology gives rise to an advanced area of nanobiotechnology that involves living entities of both prokaryotic and eukaryotic origin, such as algae, cyanobacteria, actinomycetes, bacteria, viruses, yeasts, fungi, and plants. Every biological system varies in its capabilities to supply metallic nanoparticles. However, not all biological organisms can produce nanoparticles due to their enzymatic activities and intrinsic metabolic processes. Therefore, biological entities or their extracts are used for the green synthesis of metallic nanoparticles through bio-reduction of metallic particles leading to the synthesis of nanoparticles. These biosynthesized metallic nanoparticles have a range of unlimited pharmaceutical applications including delivery of drugs or genes, detection of pathogens or proteins, and tissue engineering. The effective delivery of drugs and tissue engineering through the use of nanotechnology exhibited vital contributions in translational research related to the pharmaceutical products and their applications. Collectively, this review covers the green synthesis of nanoparticles by using various biological systems as well as their applications.
Disseminated Pelvic Actinomycosis Caused by Actinomyces Naeslundii
Authors: Džupová Olga , Kulichová Jana , Beneš Jiří
Published: October 2020

Actinomycosis is a chronic bacterial infection characterized by continuous local spread, irrespective of anatomical barriers, and granulomatous suppurative inflammation. Due to its expansive local growth, it can simulate a malignant tumour. Subsequent hematogenous dissemination to distant organs can mimic metastases and further increase suspicion for malignancy. A case of severe disseminated pelvic actinomycosis associated with intrauterine device is described here. The patient presented with a pelvic mass mimicking a tumour, bilateral ureteral obstruction, ascites, multinodular involvement of the liver, lungs and spleen, inferior vena cava thrombosis and extreme cachexia. Actinomycosis was diagnosed by liver biopsy and confirmed by culture of Actinomyces naeslundii from extracted intrauterine contraceptive device (IUD). Prolonged treatment with aminopenicillin and surgery resulted in recovery with moderate sequelae.
Spread of Antimicrobial Resistance by Salmonella enterica Serovar Choleraesuis between Close Domestic and Wild Environments
Authors: Gil Molino María , García Alfredo , Zurita Sofía Gabriela , Martín-Cano Francisco Eduardo , García-Jiménez Waldo , Risco David , Rey Joaquín , Fernández-Llario Pedro , Quesada Alberto
Published: October 2020

The Salmonellaenterica serovar Choleraesuis affects domestic pig and wild boar (WB), causing clinical salmonellosis. Iberian swine production is based on a free-range production system where WB and Iberian pig (IP) share ecosystems. This study focuses on the negative impact on the pork industry of infections due to this serotype, its role in the spread of antibiotic resistance, and its zoonotic potential. Antibiotic resistance (AR) and genetic relationships were analyzed among 20 strains of S. Choleraesuis isolated from diseased WB and IP sampled in the southwest region of the Iberian Peninsula. AR was studied using the Kirby–Bauer method with the exception of colistin resistance, which was measured using the broth microdilution reference method. Resistance and Class 1 integrase genes were measured using PCR, and the genetic relationship between isolates and plasmid content by pulsed field gel electrophoresis. The results show a higher incidence of AR in isolates from IP. Phylogenetic analysis revealed seven profiles with two groups containing isolates from IP and WB, which indicates circulation of the same clone between species. Most pulsotypes presented with one plasmid of the same size, indicating vertical transmission. AR determinants blaTEM and tetA were routinely found in IP and WB, respectively. One isolate from IP expressed colistin resistance and presented the mcr-1 gene carried by a plasmid. This study suggests that S. Choleraesuis circulates between WB and IP living in proximity, and also that the mobilization of AR genes by plasmids is low. Furthermore, the detection of plasmid-mediated colistin resistance in bacteria from IP is alarming and should be monitored.
Body Posture Defects and Body Composition in School-Age Children
Authors: Wilczyński Jacek , Lipińska-Stańczak Magdalena , Wilczyński Igor
Published: October 2020

The aim of the study was to assess the relationship between the shape of the anteriorposterior spinal curvature and body composition in schoolchildren. The study included 257 children, aged 11–12. Correct spinal curvature was established in 106 (41.08%) subjects. Other types included: decreased kyphosis and correct lordosis—40 participants (15.50%), correct kyphosis and decreased lordosis—24 individuals (9.30%), increased kyphosis and correct lordosis—17 subjects (6.59%), correct kyphosis and increased lordosis—22 children (8.53%), decreased kyphosis and decreased lordosis—32 people (12.40%), decreased kyphois and increased lordosis—four of the examined subjects (1.55%) increased kyphosis and lordosis—13 people (5.04%). In addition, 134 (51.94%) demonstrated scoliotic posture and eight (3.10%) scoliosis. There were significant relationships between the shape of the anteriorposterior curvatures and body composition in schoolchildren. Those with a strong body build (predominance of mesomorphs) were generally characterised by the correct formation of these curvatures. In contrast, lean subjects (with the predominance of ectomorphic factors) were more likely to experience abnormalities. No correlations with body composition were observed in the group with scoliotic posture or scoliosis. Both in the prevention and correction of postural defects, one should gradually move away from one-sided, usually one-system, therapeutic effects. An approach that takes into account both somatic and neurophysiological factors seems appropriate. With the correct body composition and structure, shaping the habit of correct posture is much easier.
Theoretical Study on Cryogen Spray Cooling in Laser Treatment of Ota’s Nevus: Comparison and Optimization of R134a, R404A and R32
Authors: Tian Jiameng , Chen Bin , Zhou Zhifu , Li Dong
Published: October 2020

Cryogen spray cooling (CSC) could be applied clinically for the laser therapy of Ota’s nevus, a dermal hyperplastic pigmented disease with a morbidity rate of 0.1–0.6% in the Asian population. An accurate, efficient, complete simulation system that considers the entire spray cooling process, including cryogen flow in the tube nozzle, spray dynamics and internal phase change heat transfer (cold injury) in skin tissue, was established to determine suitable cryogen and cooling parameters. The optimum spray distances for R134a, R404A and R32 were determined to be 66.0, 43.1 and 22.5 mm, respectively. The corresponding maximum surface heat fluxes were 363.5, 459.9, and 603.6 kW∙m−2, respectively. The maximum surface heat flux of R32 with small spray distance was 1.66 times as large as that of R134a, indicating the potentially good cooling performance and precise targeted cooling of R32 during the laser therapy of Ota’s nevus. The cooling durations that caused cold injury of skin tissue were 2.3, 1.4, and 1.1 s for R134a, R404A, and R32, respectively. The interval between CSC and laser irradiation was optimized to 90–162 ms for R134a, R404A and R32, in consideration of the cooling effect, depth, uniformity, and risk of cold injury.
Environmental and Economic Impact of the Antifreeze Agents in Geothermal Heat Exchangers
Authors: Bartolini Nicola , Casasso Alessandro , Bianco Carlo , Sethi Rajandrea
Published: October 2020

Borehole heat exchangers (BHEs) generally employ water-antifreeze solutions to allow working fluid temperatures to fall below 0 °C. However, some local regulations have forbidden antifreeze additives (even non-toxic ones) to avoid groundwater pollution in case of pipe leakage. This paper presents a techno-economic and environmental analysis of four different fluids: propylene glycol at 25% and 33% weight concentrations, calcium chloride at 20% weight concentration (CaCl2 20%), and pure water. Thermal loads from 36 case studies in six different climate zones are used to perform BHE sizing and compare the abovementioned fluids from the economic, operational, and environmental points of view. The economic analysis and the carbon footprint assessment are performed on a life cycle of 25 years considering the installation (BHE drilling, fluid) and operation (heat pump and ground-side circulation pump energy demand, fluid replacement) of the simulated GSHPs. Results highlight that using pure water as a heat carrier fluid is convenient for cooling-dominated buildings but, for heating-dominated buildings, this choice leads to a noticeable increase of the BHE needed length which is not compensated by the lower operational costs. On the other hand, avoiding the use of antifreeze additives generally leads to a reduction of the lifetime carbon footprint, with a few exceptions in very cold climates. CaCl2 20% proves to be a good choice in most cases, both from the economic and the environmental points of view, as it allows a strong reduction of the installed BHE length in cold climates with a low additional cost and carbon footprint.
Ecological Scarcity Based Impact Assessment for a Decentralised Renewable Energy System
Authors: Lambrecht Hendrik , Lewerenz Steffen , Hottenroth Heidi , Tietze Ingela , Viere Tobias
Published: October 2020

Increasing the share of renewable energies in electricity and heat generation is the cornerstone of a climate-friendly energy transition. However, as renewable technologies rely on diverse natural resources, the design of decarbonized energy systems inevitably leads to environmental trade-offs. This paper presents the case study of a comprehensive impact assessment for different future development scenarios of a decentralized renewable energy system in Germany. It applies an adapted ecological scarcity method (ESM) that improves decision-support by ranking the investigated scenarios and revealing their main environmental shortcomings: increased mineral resource use and pollutant emissions due to required technical infrastructure and a substantial increase in land use due to biomass combustion. Concerning the case study, the paper suggests extending the set of considered options, e.g., towards including imported wind energy. More generally, the findings underline the need for a comprehensive environmental assessment of renewable energy systems that integrate electricity supply with heating, cooling, and mobility. On a methodical level, the ESM turns out to be a transparent and well adaptable method to analyze environmental trade-offs from renewable energy supply. It currently suffers from missing quantitative targets that are democratically sufficiently legitimized. At the same time, it can provide a sound basis for an informed discussion on such targets.
Development and Application of Ion Current/Cylinder Pressure Cooperative Combustion Diagnosis and Control System
Authors: Zhu Denghao , Deng Jun , Wang Jinqiu , Wang Shuo , Zhang Hongyu , Andert Jakob , Li Liguang
Published: October 2020

The application of advanced technologies for engine efficiency improvement and emissions reduction also increase the occurrence possibility of abnormal combustions such as incomplete combustion, misfire, knock or pre-ignition. Novel promising combustion modes, which are basically dominated by chemical reaction kinetics show a major difficulty in combustion control. The challenge in precise combustion control is hard to overcome by the traditional engine map-based control method because it cannot monitor the combustion state of each cycle, hence, real-time cycle-resolved in-cylinder combustion diagnosis and control are required. In the past, cylinder pressure and ion current sensors, as the two most commonly used sensors for in-cylinder combustion diagnosis and control, have enjoyed a seemingly competitive relationship, so all related researches only use one of the sensors. However, these two sensors have their own unique features. In this study, the idea is to combine the information obtained from both sensors. At first, two kinds of ion current detection system are comprehensively introduced and compared at the hardware level and signal level. The most promising variant (the DC-Power ion current detection system) is selected for the subsequent experiments. Then, the concept of ion current/cylinder pressure cooperative combustion diagnosis and control system is illustrated and implemented on the engine prototyping control unit. One application case of employing this system for homogenous charge compression ignition abnormal combustion control and its stability improvement is introduced. The results show that a combination of ion current and cylinder pressure signals can provide richer and also necessary information for combustion control. Finally, ion current and cylinder pressure signals are employed as inputs of artificial neural network (ANN) models for combustion prediction. The results show that the combustion prediction performance is better when the inputs are a combination of both signals, instead of using only one of them. This offline analysis proves the feasibility of using an ANN-based model whose inputs are a combination of ion current and pressure signals for better prediction accuracy.
DC-DC High-Voltage-Gain Converters with Low Count of Switches and Common Ground
Authors: Stala Robert , Waradzyn Zbigniew , Folmer Szymon
Published: October 2020

This paper presents a new concept and research results of DC-DC high-voltage-gain, high-frequency step-up resonant converters. The proposed topologies are optimized towards minimizing the number of switches and improvements in efficiency. Another relevant advantage of such type of converters is that they have a common input and output negative point. The proposed converters are based on the resonant switched-capacitor voltage multiplier circuit, and that is why they are compared with a classic converter from this family. The included results show the operating principle, possible switching methods with the consideration of their impact on the voltage gain level, as well as the voltage and current ripples. The operating concepts and analytical calculations are confirmed by simulation and experimental results.
Intussusceptive Angiogenesis and Peg–Socket Junctions between Endothelial Cells and Smooth Muscle Cells in Early Arterial Intimal Thickening
Authors: Díaz-Flores Lucio , Gutiérrez Ricardo , García Mª Pino , Gayoso Sara , Carrasco José Luís , Díaz-Flores Lucio. , González-Gómez Miriam , Madrid Juan Francisco
Published: October 2020

Angiogenesis in arterial intimal thickening (AIT) has been considered mainly in late AIT stages and only refers to sprouting angiogenesis. We assess angiogenesis during early AIT development and the occurrence of the intussusceptive type. For this purpose, we studied AIT development in (a) human arteries with vasculitis in gallbladders with acute cholecystitis and urgent (n = 25) or delayed (n = 20) cholecystectomy, using immunohistochemical techniques and (b) experimentally occluded arterial segments (n = 56), using semithin and ultrathin sections and electron microscopy. The results showed transitory angiogenic phenomena, with formation of an important microvasculature, followed by vessel regression. In addition to the sequential description of angiogenic and regressive findings, we mainly contribute (a) formation of intravascular pillars (hallmarks of intussusception) during angiogenesis and vessel regression and (b) morphological interrelation between endothelial cells (ECs) in the arterial wall and vascular smooth muscle cells (VSMCs), which adopt a pericytic arrangement and establish peg-and-socket junctions with ECs. In conclusion, angiogenesis and vessel regression play an important role in AIT development in the conditions studied, with participation of intussusceptive angiogenesis during the formation and regression of a provisional microvasculature and with morphologic interrelation between ECs and VSMCs.
Recent Advances in Single-Particle Electron Microscopic Analysis of Autophagy Degradation Machinery
Authors: Cheung Yiu Wing Sunny , Nam Sung-Eun , Yip Calvin K.
Published: October 2020

Macroautophagy (also known as autophagy) is a major pathway for selective degradation of misfolded/aggregated proteins and damaged organelles and non-selective degradation of cytoplasmic constituents for the generation of power during nutrient deprivation. The multi-step degradation process, from sequestering cytoplasmic cargo into the double-membrane vesicle termed autophagosome to the delivery of the autophagosome to the lysosome or lytic vacuole for breakdown, is mediated by the core autophagy machinery composed of multiple Atg proteins, as well as the divergent sequence family of selective autophagy receptors. Single-particle electron microscopy (EM) is a molecular imaging approach that has become an increasingly important tool in the structural characterization of proteins and macromolecular complexes. This article summarizes the contributions single-particle EM have made in advancing our understanding of the core autophagy machinery and selective autophagy receptors. We also discuss current technical challenges and roadblocks, as well as look into the future of single-particle EM in autophagy research.
Cell Communications among Microorganisms, Plants, and Animals: Origin, Evolution, and Interplays
Authors: Combarnous Yves , Nguyen Thi Mong Diep
Published: October 2020

Cellular communications play pivotal roles in multi-cellular species, but they do so also in uni-cellular species. Moreover, cells communicate with each other not only within the same individual, but also with cells in other individuals belonging to the same or other species. These communications occur between two unicellular species, two multicellular species, or between unicellular and multicellular species. The molecular mechanisms involved exhibit diversity and specificity, but they share common basic features, which allow common pathways of communication between different species, often phylogenetically very distant. These interactions are possible by the high degree of conservation of the basic molecular mechanisms of interaction of many ligand–receptor pairs in evolutionary remote species. These inter-species cellular communications played crucial roles during Evolution and must have been positively selected, particularly when collectively beneficial in hostile environments. It is likely that communications between cells did not arise after their emergence, but were part of the very nature of the first cells. Synchronization of populations of non-living protocells through chemical communications may have been a mandatory step towards their emergence as populations of living cells and explain the large commonality of cell communication mechanisms among microorganisms, plants, and animals.
PPARs and Microbiota in Skeletal Muscle Health and Wasting
Authors: Manickam Ravikumar , Duszka Kalina , Wahli Walter
Published: October 2020

Skeletal muscle is a major metabolic organ that uses mostly glucose and lipids for energy production and has the capacity to remodel itself in response to exercise and fasting. Skeletal muscle wasting occurs in many diseases and during aging. Muscle wasting is often accompanied by chronic low-grade inflammation associated to inter- and intra-muscular fat deposition. During aging, muscle wasting is advanced due to increased movement disorders, as a result of restricted physical exercise, frailty, and the pain associated with arthritis. Muscle atrophy is characterized by increased protein degradation, where the ubiquitin-proteasomal and autophagy-lysosomal pathways, atrogenes, and growth factor signaling all play an important role. Peroxisome proliferator-activated receptors (PPARs) are members of the nuclear receptor family of transcription factors, which are activated by fatty acids and their derivatives. PPARs regulate genes that are involved in development, metabolism, inflammation, and many cellular processes in different organs. PPARs are also expressed in muscle and exert pleiotropic specialized responses upon activation by their ligands. There are three PPAR isotypes, viz., PPARα, -β/δ, and -γ. The expression of PPARα is high in tissues with effective fatty acid catabolism, including skeletal muscle. PPARβ/δ is expressed more ubiquitously and is the predominant isotype in skeletal muscle. It is involved in energy metabolism, mitochondrial biogenesis, and fiber-type switching. The expression of PPARγ is high in adipocytes, but it is also implicated in lipid deposition in muscle and other organs. Collectively, all three PPAR isotypes have a major impact on muscle homeostasis either directly or indirectly. Furthermore, reciprocal interactions have been found between PPARs and the gut microbiota along the gut–muscle axis in both health and disease. Herein, we review functions of PPARs in skeletal muscle and their interaction with the gut microbiota in the context of muscle wasting.
Cholesterol-Rich Microdomains Contribute to PAR1 Signaling in Platelets Despite a Weak Localization of the Receptor in These Microdomains
Authors: Rabani Vahideh , Lagoutte-Renosi Jennifer , Series Jennifer , Valot Benoit , Xuereb Jean-Marie , Davani Siamak
Published: October 2020

Platelet protease-activated receptor 1 (PAR1) is a cell surface G-protein-coupled receptor (GPCR) that acts as a thrombin receptor promoting platelet aggregation. Targeting the PAR1 pathway by vorapaxar, a PAR1 antagonist, leads to a reduction in ischemic events in cardiovascular patients with a history of myocardial infarction or with peripheral arterial disease. In platelets, specialized microdomains highly enriched in cholesterol act as modulators of the activity of several GPCRs and play a pivotal role in the signaling pathway. However, their involvement in platelet PAR1 function remains incompletely characterized. In this context, we aimed to investigate whether activation of PAR1 in human platelets requires its localization in the membrane cholesterol-rich microdomains. Using confocal microscopy, biochemical isolation, and proteomics approaches, we found that PAR1 was not localized in cholesterol-rich microdomains in resting platelets, and only a small fraction of the receptor relocated to the microdomains following its activation. Vorapaxar treatment increased the level of PAR1 at the platelet surface, possibly by reducing its endocytosis, while its colocalization with cholesterol-rich microdomains remained weak. Consistent with a cholesterol-dependent activation of Akt and p38 MAP kinase in thrombin receptor-activating peptide (TRAP)-activated platelets, the proteomic data of cholesterol-rich microdomains isolated from TRAP-activated platelets showed the recruitment of proteins contributing to these signaling pathways. In conclusion, contrary to endothelial cells, we found that PAR1 was only weakly present in cholesterol-rich microdomains in human platelets but used these microdomains for efficient activation of downstream signaling pathways following TRAP activation.
Coumarin-Modified CQDs for Biomedical Applications—Two-Step Synthesis and Characterization
Authors: Janus Łukasz , Radwan-Pragłowska Julia , Piątkowski Marek , Bogdał Dariusz
Published: October 2020

Waste biomass such as lignin constitutes a great raw material for eco-friendly carbon quantum dots (CQDs) synthesis, which find numerous applications in various fields of industry and medicine. Carbon nanodots, due to their unique luminescent properties as well as water-solubility and biocompatibility, are superior to traditional organic dyes. Thus, obtainment of CQDs with advanced properties can contribute to modern diagnosis and cell visualization method development. In this article, a new type of coumarin-modified CQD was obtained via a hybrid, two-step pathway consisting of hydrothermal carbonization and microwave-assisted surface modification with coumarin-3-carboxylic acid and 7-(Diethylamino) coumarin-3-carboxylate. The ready products were characterized over their chemical structure and morphology. The nanomaterials were confirmed to have superior fluorescence characteristics and quantum yield up to 18.40%. They also possessed the ability of biomolecules and ion detection due to the fluorescence quenching phenomena. Their lack of cytotoxicity to L929 mouse fibroblasts was confirmed by XTT assay. Moreover, the CQDs were proven over their applicability in real-time bioimaging. Obtained results clearly demonstrated that proposed surface-modified carbon quantum dots may become a powerful tool applicable in nanomedicine and pharmacy.
Developmental Roles of FUSE Binding Protein 1 (Fubp1) in Tooth Morphogenesis
Authors: Aryal Yam Prasad , Neupane Sanjiv , Kim Tae-Young , Lee Eui-Seon , Pokhrel Nitin Kumar , Yeon Chang-Yeol , Kim Ji-Youn , An Chang-Hyeon , An Seo-Young , Park Eui-Kyun , Ha Jung-Hong , Jung Jae-Kwang , Yamamoto Hitoshi , Cho Sung-Won , Lee Sanggyu , Kim Do-Yeon , Kwon Tae-Yub , Lee Youngkyun , Sohn Wern-Joo , Kim Jae-Young
Published: October 2020

FUSE binding protein 1 (Fubp1), a regulator of the c-Myc transcription factor and a DNA/RNA-binding protein, plays important roles in the regulation of gene transcription and cellular physiology. In this study, to reveal the precise developmental function of Fubp1, we examined the detailed expression pattern and developmental function of Fubp1 during tooth morphogenesis by RT-qPCR, in situ hybridization, and knock-down study using in vitro organ cultivation methods. In embryogenesis, Fubp1 is obviously expressed in the enamel organ and condensed mesenchyme, known to be important for proper tooth formation. Knocking down Fubp1 at E14 for two days, showed the altered expression patterns of tooth development related signalling molecules, including Bmps and Fgf4. In addition, transient knock-down of Fubp1 at E14 revealed changes in the localization patterns of c-Myc and cell proliferation in epithelium and mesenchyme, related with altered tooth morphogenesis. These results also showed the decreased amelogenin and dentin sialophosphoprotein expressions and disrupted enamel rod and interrod formation in one- and three-week renal transplanted teeth respectively. Thus, our results suggested that Fubp1 plays a modulating role during dentinogenesis and amelogenesis by regulating the expression pattern of signalling molecules to achieve the proper structural formation of hard tissue matrices and crown morphogenesis in mice molar development.
Mechanical and Biocompatibility Properties of Calcium Phosphate Bioceramics Derived from Salmon Fish Bone Wastes
Authors: Bas Merve , Daglilar Sibel , Kuskonmaz Nilgun , Kalkandelen Cevriye , Erdemir Gokce , Kuruca Serap E. , Tulyaganov Dilshat , Yoshioka Tomohiko , Gunduz Oguzhan , Ficai Denisa , Ficai Anton
Published: October 2020

Natural calcium phosphates derived from fish wastes are a promising material for biomedical application. However, their sintered ceramics are not fully characterized in terms of mechanical and biological properties. In this study, natural calcium phosphate was synthesized through a thermal calcination process from salmon fish bone wastes. The salmon-derived calcium phosphates (sCaP) were sintered at different temperatures to obtain natural calcium phosphate bioceramics and then were investigated in terms of their microstructure, mechanical properties and biocompatibility. In particular, this work is concerned with the effects of grain size on the relative density and microhardness of the sCaP bioceramics. Ca/P ratio of the sintered sCaP ranged from 1.73 to 1.52 when the sintering temperature was raised from 1000 to 1300 °C. The crystal phase of all the sCaP bioceramics obtained was biphasic and composed of hydroxyapatite (HA) and tricalcium phosphate (TCP). The density and microhardness of the sCaP bioceramics increased in the temperature interval 1000–1100 °C, while at temperatures higher than 1100 °C, these properties were not significantly altered. The highest compressive strength of 116 MPa was recorded for the samples sintered at 1100 °C. In vitro biocompatibility was also examined in the behavior of osteosarcoma (Saos-2) cells, indicating that the sCaP bioceramics had no cytotoxicity effect. Salmon-derived biphasic calcium phosphates (BCP) have the potential to contribute to the development of bone substituted materials.
Changes in Honey Bee Head Proteome in Response to Dietary 24-Methylenecholesterol
Authors: Chakrabarti Priyadarshini , Sagili Ramesh R.
Published: October 2020

Phytosterols are important micronutrients that are precursors of important molting hormones and help maintain cellular membrane integrity in insects including bees. Previous research has shown that 24-methylenecholesterol is a key phytosterol that enhances honey bee longevity and improves nurse bee physiology. Nurse bees have the ability to selectively transfer this sterol to developing larvae through brood food. This study examines the physiological impacts of 24-methylenecholesterol on nurse bees, by analyzing the protein profiles of nurse bee heads upon dietary sterol manipulation. Dietary experimental groups consisting of newly emerged honey bees were provided with varying concentrations of 24-methylenecholesterol for three weeks. At the end of the study, honey bees were collected and proteomic analysis was performed on honey bee heads. A total of 1715 proteins were identified across experimental groups. The mean relative abundances of nutritional marker proteins (viz. major royal jelly proteins 1, 4, 5, 7) were higher in experimental groups supplemented with higher dietary sterol concentrations, when compared with the control dietary group. The mean relative abundances of important enzymatic proteins (aminopeptidase and calcium-transporting ATPase) were higher in control groups, whereas mean relative abundances of oxysterol-binding protein and fatty acid-binding protein were higher in higher dietary sterol groups.
A Comparative Assessment of Collision Risk of Manned and Unmanned Vessels
Authors: Wu Qing , Wang Tengfei , Diaconeasa Mihai A. , Mosleh Ali , Wang Yang
Published: October 2020

It is expected that the prototypes of unmanned merchant ships will be deployed in the next few years. However, there is no specific research on whether the introduction of unmanned ships will reduce the risk of ship collision accidents in which communication between vessels is critical. This work constitutes an attempt to bridge the gap identified above by applying the Hybrid Causal Logic (HCL) methodology to model general-level collision scenarios of unmanned ships. The HCL methodology has been selected for its proven applicability to risk assessments, even when empirical data may be insufficient. Collision scenarios involving unmanned ships have been created in which manned ships of the conventional collision scenario HCL model are replaced with unmanned ships. Then, collision scenarios capturing the interactions between a manned ship and an unmanned ship were modeled. By comparing the qualitative and quantitative results of the different scenarios, we can see that the introduction of unmanned ships may effectively reduce the occurrence of ship collision accidents.
The Contribution of Small Phytoplankton Communities to the Total Dissolved Inorganic Nitrogen Assimilation Rates in the East/Japan Sea: An Experimental Evaluation
Authors: Bhavya Panthalil S. , Kang Jae Joong , Jang Hyo Keun , Joo HuiTae , Lee Jae Hyung , Lee Jang Han , Park Jung Woo , Kim Kwanwoo , Kim Hyung Chul , Lee Sang Heon
Published: October 2020

As a part of Korean-Russian joint expeditions in the East/Japan Sea during 2012 and 2015, a set of total and small (<2 μm) phytoplankton NO3− and NH4+ uptake rate estimations were carried out. The study aimed to assess the spatio-temporal variations in dissolved inorganic nitrogen (DIN) assimilation by the total and small phytoplankton. The results show that the total NO3− uptake rates during 2012 varied between 0.001 and 0.150 μmol NL−1h−1 (mean ± SD = 0.034 ± 0.033) and that the total NH4+ uptake rates ranged between 0.002 and 0.707 μmol NL−1h−1 (mean ± SD = 0.200 ± 0.158). The total uptake rates during 2015 were ranged from 0.003 to 0.530 (mean ± S.D. = 0.117 ± 0.120 μmol NL−1h−1) for NO3− and from 0.008 to 1.17 (mean ± S.D. = 0.199 ± 0.266 NL−1h−1) for NH4+. The small phytoplankton NO3− and NH4+ uptake rates during 2015 ranged between 0.001 and 0.164 (mean ± S.D. = 0.033 ± 0.036) μmol NL−1h−1 and 0.010–0.304 (mean ± S.D. = 0.101 ± 0.073) μmol NL−1h−1, respectively. Small phytoplankton’s contribution to the total depth-integrated NO3− and NH4+ uptake rates ranged from 10.24 to 59.36% and from 30.21 to 68.55%, respectively. The significant negative relationship observed between the depth-integrated total NO3− and NH4+ uptake rates and small phytoplankton contributions indicates a possible decline in the DIN assimilation rates under small phytoplankton dominance. The results from the present study highlight the possibility of a reduction in the total DIN assimilation process in the East/Japan Sea when small phytoplankton dominate under strong thermal stratification due to sea surface warming. The present study’s findings agree with the model projections, which suggested a decline in primary production in the global warming scenario.
Bioprospecting White-Rot Basidiomycete Irpex lacteus for Improved Extraction of Lignocellulose-Degrading Enzymes and Their Further Application
Authors: Mezule Linda , Civzele Anna
Published: October 2020

Lignocellulosic biomass can be used as a source for energy, fuel and valuable chemical production. From all available technologies, biological approaches have been recognized as the most environmentally friendly and sustainable ones. At the same time, high conversion costs, low efficiency and environmental issues still hinder the introduction of biological processes into industrial scale manufacturing. The aim of this study was to determine the most suitable enzyme cocktail recovery conditions from a biomass–fungal culture of the white-rot basidiomycete Irpex lacteus. Subsequent evaluation of the overall enzyme cocktail efficiency to release fermentable carbohydrates from biomass showed that prolonged fungal cultivation decreases the quality of the produced enzyme cocktail. At the same time, introduction of ultrasound pre-treatment during enzyme extraction improved the recovered enzyme cocktail efficiency in converting biomass to fermentable sugars, yielding up to 0.25 g of fermentable sugar per g dry hay biomass and up to 0.11 g per g dried straw or microalgae substrates. The results demonstrated that the production of lignocellulose-degrading enzymes from fungi is more sensitive than previously described, especially in terms of fungal growth, culture sterility and incubation conditions.
On the Dirichlet Problem with Corner Singularity
Authors: Rukavishnikov Viktor A. , Rukavishnikova Elena I.
Published: October 2020

We consider the Dirichlet problem for an elliptic equation with a singularity. The singularity of the solution to the problem is caused by the presence of a re-entrant corner at the boundary of the domain. We define an Rν-generalized solution for this problem. This allows for the construction of numerical methods for finding an approximate solution without loss of accuracy. In this paper, the existence and uniqueness of the Rν-generalized solution in set W∘2,α1(Ω,δ) is proven. The Rν-generalized solution is the same for different parameters ν.
On the Geometric Mean Method for Incomplete Pairwise Comparisons
Authors: Kułakowski Konrad
Published: October 2020

One of the most popular methods of calculating priorities based on the pairwise comparisons matrices (PCM) is the geometric mean method (GMM). It is equivalent to the logarithmic least squares method (LLSM), so some use both names interchangeably, treating it as the same approach. The main difference, however, is in the way the calculations are done. It turns out, however, that a similar relationship holds for incomplete matrices. Based on Harker’s method for the incomplete PCM, and using the same substitution for the missing entries, it is possible to construct the geometric mean solution for the incomplete PCM, which is fully compatible with the existing LLSM for the incomplete PCM. Again, both approaches lead to the same results, but the difference is how the final solution is computed. The aim of this work is to present in a concise form, the computational method behind the geometric mean method (GMM) for an incomplete PCM. The computational method is presented to emphasize the relationship between the original GMM and the proposed solution. Hence, everyone who knows the GMM for a complete PCM should easily understand its proposed extension. Theoretical considerations are accompanied by a numerical example, allowing the reader to follow the calculations step by step.
Efficient Numerical Scheme for the Solution of Tenth Order Boundary Value Problems by the Haar Wavelet Method
Authors: Amin Rohul , Shah Kamal , Khan Imran , Asif Muhammad , Salimi Mehdi , Ahmadian Ali
Published: October 2020

In this paper, an accurate and fast algorithm is developed for the solution of tenth order boundary value problems. The Haar wavelet collocation method is applied to both linear and nonlinear boundary value problems. In this technqiue, the tenth order derivative in boundary value problem is approximated using Haar functions and the process of integration is used to obtain the expression of lower order derivatives and approximate solution for the unknown function. Three linear and two nonlinear examples are taken from literature for checking validation and the convergence of the proposed technique. The maximum absolute and root mean square errors are compared with the exact solution at different collocation and Gauss points. The experimental rate of convergence using different number of collocation points is also calculated, which is nearly equal to 2.
Finite Difference Method for Two-Sided Two Dimensional Space Fractional Convection-Diffusion Problem with Source Term
Authors: Anley Eyaya Fekadie , Zheng Zhoushun
Published: October 2020

In this paper, we have considered a numerical difference approximation for solving two-dimensional Riesz space fractional convection-diffusion problem with source term over a finite domain. The convection and diffusion equation can depend on both spatial and temporal variables. Crank-Nicolson scheme for time combined with weighted and shifted Grünwald-Letnikov difference operator for space are implemented to get second order convergence both in space and time. Unconditional stability and convergence order analysis of the scheme are explained theoretically and experimentally. The numerical tests are indicated that the Crank-Nicolson scheme with weighted shifted Grünwald-Letnikov approximations are effective numerical methods for two dimensional two-sided space fractional convection-diffusion equation.
Erysipelas Complicated with Acute Exudative Pericarditis
Authors: Gečaitė Akvilė , Vainalavičiūtė Aušra , Rekienė Daiva Emilija , Jankauskienė Laima , Naudžiūnas Albinas
Published: October 2020

Erysipelas is a common skin infection of the upper dermis. Its most common complications are local; these include abscess formation, skin necrosis, etc. In the present article, we introduce a case of a 75-year-old patient with erysipelas of the face complicated with acute exudative pericarditis. The patient came to Kaunas Clinical Hospital complaining of extreme fatigue and fever, oedema of the left side of the face, and erythema typical for erysipelas. The patient also felt sternum and epigastric pain, especially during breathing, and dyspnoea. Heart work was rhythmic 100 bpm; blood pressure was 142/70 mmHg. Pericardial friction rub was heard over the left sternal border. There were no alterations in other systems. In the electrocardiogram, concave ST segment elevation in leads II, III, and aVF was identified. In addition, during hospitalisation, the patient experienced atrial fibrillation paroxysm, which was treated with amiodarone intravenously. The blood test showed C-reactive protein: 286 mg/L; white blood cells: 20 × 109/L; troponin I was within the normal range. During echocardiography, pericardial fluid in pericardial cavity was identified. As no changes in troponin I were observed, according to the ST segment elevation, the woman was diagnosed with erysipelas of the left side of the face complicated with acute exudative pericarditis. Antibacterial treatment of cephalosporins was administered. After the treatment, C-reactive protein decreased to 27.8 mg/L; whereas, in the electrocardiogram, the return of the ST segment to the isoline was observed, and pericardial fluid resorbed from the pericardial cavity. To the best of the authors’ knowledge, this case is a rare combination of erysipelas complicated with acute exudative pericarditis.
Effects of the CB1 Receptor Antagonists AM6545 and AM4113 on Insulin Resistance in a High-Fructose High-Salt Rat Model of Metabolic Syndrome
Authors: Eid Basma G. , Neamatallah Thikryat , Hanafy Abeer , El-Bassossy Hany M. , Aldawsari Hibah M. , Vemuri Kiran , Makriyannis Alexandros
Published: October 2020

Background and Objectives: Insulin resistance (IR) is a serious condition leading to development of diabetes and cardiovascular complications. Hyper-activation of cannabinoid receptors-1 (CB1) has been linked to the development of metabolic disorders such as IR. Therefore, the effect of blocking CB1 on the development of IR was investigated in the present study. Materials and Methods: A 12-week high-fructose/high-salt feeding model of metabolic syndrome was used to induce IR in male Wistar rats. For this purpose, two different CB1-antagonists were synthesized and administered to the rats during the final four weeks of the study, AM6545, the peripheral neutral antagonist and AM4113, the central neutral antagonist. Results: High-fructose/salt feeding for 12 weeks led to development of IR while both AM6545 and AM4113, administered in the last 4 weeks, significantly inhibited IR. This was correlated with increased animal body weight wherein both AM6545 and AM4113 decreased body weight in IR animals but with loss of IR/body weight correlation. While IR animals showed significant elevations in serum cholesterol and triglycerides with no direct correlation with IR, both AM6545 and AM4113 inhibited these elevations, with direct IR/cholesterol correlation in case of AM6545. IR animals had elevated serum uric acid, which was reduced by both AM6545 and AM4113. In addition, IR animals had decreased adiponectin levels and elevated liver TNFα content with strong IR/adiponectin and IR/TNFα correlations. AM6545 inhibited the decreased adiponectin and the increased TNFα levels and retained the strong IR/adiponectin correlation. However, AM4113 inhibited the decreased adiponectin and the increased TNFα levels, but with loss of IR/adiponectin and IR/TNFα correlations. Conclusions: Both CB1 neutral antagonists alleviated IR peripherally, and exerted similar effects on rats with metabolic syndrome. They also displayed anti-dyslipidemic, anti-hyperurecemic and anti-inflammatory effects. Overall, these results should assist in the development of CB1 neutral antagonists with improved safety profiles for managing metabolic disorders.
Phylogeography of 27,000 SARS-CoV-2 Genomes: Europe as the Major Source of the COVID-19 Pandemic
Authors: Rito Teresa , Richards Martin B. , Pala Maria , Correia-Neves Margarida , Soares Pedro A.
Published: October 2020

The novel coronavirus SARS-CoV-2 emerged from a zoonotic transmission in China towards the end of 2019, rapidly leading to a global pandemic on a scale not seen for a century. In order to cast fresh light on the spread of the virus and on the effectiveness of the containment measures adopted globally, we used 26,869 SARS-CoV-2 genomes to build a phylogeny with 20,247 mutation events and adopted a phylogeographic approach. We confirmed that the phylogeny pinpoints China as the origin of the pandemic with major founders worldwide, mainly during January 2020. However, a single specific East Asian founder underwent massive radiation in Europe and became the main actor of the subsequent spread worldwide during March 2020. This lineage accounts for the great majority of cases detected globally and even spread back to the source in East Asia. Despite an East Asian source, therefore, the global pandemic was mainly fueled by its expansion across and out of Europe. It seems likely that travel bans established throughout the world in the second half of March helped to decrease the number of intercontinental exchanges, particularly from mainland China, but were less effective between Europe and North America where exchanges in both directions are visible up to April, long after bans were imposed.
What Do You Meme, Professor? An Experiment Using “Memes” in Pharmacy Education
Authors: Brown Joshua D.
Published: October 2020

Memes are social or cultural constructs and ideas dispersed from person to person. The modern definition of memes applies to images, photographs, or videos shared on digital platforms juxtaposed with text that utilizes the emotion, meaning, or joke behind the original “meme” to communicate the author’s message. Younger generations of learners are more prone to utilize digital and social media to distribute and consume information and use of these platforms has increased in modern classrooms. However, there are few examples of using memes for educational purposes, including student-generated content, and no known examples in pharmacy education. This commentary introduces the concept of a meme and describes an attempt to incorporate a student-generated meme assignment in a pharmacy course. Experiences and lessons learned are discussed as words of caution in incorporating and evaluating memes or other informal communication tools for educational purposes.
Ovarian Cancer: Tumor-Specific Urinary Micro-Peptides Profiling as Potential Biomarkers for Early Diagnosis
Authors: Murgan Sulafa S. , Abd Elaziz Faisal J. , Nasr Abubakr M. A. , Elfaki Mona E. E. , Khalil Eltahir A. G.
Published: October 2020

Ovarian cancer is the second major lethal gynecologic malignancy in developing countries. This study aimed to characterize urinary micro-peptides as potential diagnostic biomarkers for ovarian cancer. In a prospective, longitudinal and case-controlled study and following informed consent, urine and plasma samples were collected from 112 women with histologically-proven ovarian cancer and 200 apparently healthy age-matched volunteers. Urinary micro-peptides were detected and sequenced using SDS-PAGE and Edman degradation technique. Serum CA125 was detected in less than a quarter (23.2%, 26/112) of patients. One or more urinary micro-peptides were detected in about two thirds of the patients (62.5%, 70/112). A total of 40 patients had three bands (57.1%, 40/70), while two bands (15 and 35 kDa) were detected in 28.6% (20/70) of the patients. Isolated 45 kDa band was seen in 14.3% (10/70). No urinary micro-peptide was detected in the volunteers. The 15 and 35 kDa bands disappeared after 6 months of regular chemotherapy, while the 45 kDa band persisted in 2.9% (2/70) of the patients after treatment. The micro-peptides were identified as: Catalase (45 kDa), α-1 Acid Glycoprotein (35 kDa) and Peroxiredoxin-2 (15 kDa). Urinary catalase, α-1 Acid Glycoprotein and Peroxiredoxin-2 can be useful biomarkers for early detection and treatment response of ovarian cancer.