bluuebunny/crossref_metadata_2025 huggingface dataset:
Omega-3 Polyunsaturated Fatty Acids Prevent Toxoplasma gondii Infection by Inducing Autophagy via AMPK Activation
Authors: Choi Jae-Won , Lee Jina , Lee Jae-Hyung , Park Byung-Joon , Lee Eun Jin , Shin Soyeon , Cha Guang-Ho , Lee Young-Ha , Lim Kyu , Yuk Jae-Min
Published: September 2019

Omega-3 polyunsaturated fatty acids (ω3-PUFAs) have potential protective activity in a variety of infectious diseases, but their actions and underlying mechanisms in Toxoplasma gondii infection remain poorly understood. Here, we report that docosahexaenoic acid (DHA) robustly induced autophagy in murine bone marrow-derived macrophages (BMDMs). Treatment of T. gondii-infected macrophages with DHA resulted in colocalization of Toxoplasma parasitophorous vacuoles with autophagosomes and reduced intracellular survival of T. gondii. The autophagic and anti-Toxoplasma effects induced by DHA were mediated by AMP-activated protein kinase (AMPK) signaling. Importantly, BMDMs isolated from Fat-1 transgenic mice, a well-known animal model capable of synthesizing ω3-PUFAs from ω6-PUFAs, showed increased activation of autophagy and AMPK, leading to reduced intracellular survival of T. gondii when compared with wild-type BMDMs. Moreover, Fat-1 transgenic mice exhibited lower cyst burden in the brain following infection with the avirulent strain ME49 than wild-type mice. Collectively, our results revealed mechanisms by which endogenous ω3-PUFAs and DHA control T. gondii infection and suggest that ω3-PUFAs might serve as therapeutic candidate to prevent toxoplasmosis and infection with other intracellular protozoan parasites.
Waist Circumference and Healthy Lifestyle Preferences/Knowledge Monitoring in a Preschool Obesity Prevention Program
Authors: Poeta Marco , Lamberti Rossella , Di Salvio Dario , Massa Grazia , Torsiello Nives , Pierri Luca , Delli Bovi Anna Pia , Di Michele Laura , Guercio Nuzio Salvatore , Vajro Pietro
Published: September 2019

Weight and body mass index (BMI) changes appear to be poor measures for assessing the success of most pediatric obesity prevention programs (POPP). The aim of this study is to evaluate the effectiveness of the preschool-age prevention program (3P) in improving and maintaining overtime preschoolers’ knowledge/preferences about healthy nutrition and physical activity (PA), and the relationship between acquired healthy behaviors and anthropometrics including waist circumference (WC). Twenty-five preschoolers underwent a 24-month healthy lifestyle multi-component pilot intervention followed by a one-year wash-out period; 25 age-matched served as controls. Anthropometric/behavioral data were monitored. After the 2-year study and wash-out, the rates of children overweight and with obesity decreased only in the intervention group, where, also, normal-weight children with visceral obesity attained WC normal values (p = 0.048). While mean values of BMI Z-scores remained unchanged in both the intervention and control groups, WC (values and percentiles) showed a significant reduction only in the intervention group. Children’s adherence to the Mediterranean diet remained acceptable among the entire sample. Although daily sweet beverage consumption remained unchanged in both groups, knowledge/preferences improved significantly more in the intervention group. In conclusion, WC may be more sensitive than BMI for monitoring preschoolers in POPP and reflects healthy behavioral changes acquired during the intervention.
Late-Evening Snack with Branched-Chain Amino Acid-Enriched Nutrients Does Not Always Inhibit Overt Diabetes in Patients with Cirrhosis: A Pilot Study
Authors: Nakanishi Keisuke , Namisaki Tadashi , Mashitani Tsuyoshi , Kaji Kosuke , Ozaki Kuniaki , Saikawa Soichiro , Sato Shinya , Inoue Takashi , Sawada Yasuhiko , Kitagawa Kou , Takaya Hiroaki , Shimozato Naotaka , Kawaratani Hideto , Moriya Kei , Akahane Takemi , Mitoro Akira , Yoshiji Hitoshi
Published: September 2019

Cirrhosis patients often have abnormal glucose metabolism. We investigated the effects of a late-evening snack (LES) with branched-chain amino acid-enriched nutrients (BCAA-EN) on glucose metabolism in cirrhosis patients. LES with BCAA-EN was administered for 1 week in 13 patients with cirrhosis and hypoalbuminemia. Blood glucose (BG) levels were measured every 15 min. The patients were divided into two groups based on BG levels: group 1 (G1, n = 11): nocturnal BG levels <200 mg/dL and group 2 (G2, n = 2): nocturnal BG levels ≥200 mg/dL. G1 had nocturnal BG levels <200 mg/dL, whereas G2 had nocturnal BG levels ≥200 mg/dL. The average BG levels did not significantly change after BCAA-EN administration in G1 (before 91.9 ± 29.0 mg/dL; after 89.0 ± 24.3 mg/dl). However, the average BG levels significantly increased after BCAA-EN administration in G2 (before 153.6 ± 43.3 mg/dL; after 200.9 ± 59.7 mg/dL) (p < 0.01). The glycated albumin level (16.6 ± 0.9% vs. 16.2 ± 2.1%), fasting immunoreactive insulin (F-IRI) level (53.9 ± 34.0 μU/mL vs. 16.5 ± 11.0 μU/mL), and homeostasis model assessment of insulin resistance (HOMA-IR) score (17.85 ± 10.58 vs. 4.02 ± 2.59) were significantly higher in G2 than in G1 (p < 0.05, p < 0.05, and p < 0.01, respectively). The quantitative insulin sensitivity check indices (0.32 ± 0.03 vs. 0.27 ± 0.02) were significantly higher in G1 than in G2 (p < 0.01). One patient in G2 was obese and had type 2 diabetes. The other patient was obese and had a high HOMA-IR score and F-IRI level. A LES with BCAA-EN does not inhibit overt diabetes in most cirrhosis patients. However, close attention should be paid to fluctuations in BG levels in cirrhosis patients who present with obesity and severe insulin resistance.
Child-Sensitive WASH Composite Score and the Nutritional Status in Cambodian Children
Authors: Manzoni Giulia , Laillou Arnaud , Samnang Chea , Hong Rathmony , Wieringa Frank T. , Berger Jacques , Poirot Etienne , Checchi Francesco
Published: September 2019

Progress in health has occurred in the past decades in Cambodia, in terms of health service access and interventions, but several indicators, including the prevalence of malnourished children, remain alarming. The causes of undernutrition are often linked to inadequate access to water, sanitation and hygiene services but limited evidence exists on the direct association between poor WASH practices and children’s’ nutritional statuses. This study investigates the relationship between water, sanitation and hygiene practices, defined as the child-sensitive composite score, and the nutritional status of children under five years old, measured as the weight-for-height z-score, mid-upper arm circumference or height-for-age z-score in six districts of Cambodia. The analysis used data from a longitudinal study, comprising extensive data collection on anthropometry, health, nutrition, WASH, and cognitive development. Chronological trends in wasting and stunting were described cross-sectionally, whereas the effect of WASH practices on the nutritional status of children over up to three consecutive study visits was examined with a linear mixed-effects model. The prevalence of wasting decreased during the study while stunting prevalence increased. A small, but significant, association was found between the WASH child-sensitive composite scores and the wasting child anthropometry indicators: weight-for-height z-score or mid-upper arm circumference. Evidence for an association with height-for-age z-score, detecting stunted children, was found when the independent variable was quantified according to global, but not national, guidelines. This study reinforces discordant existing evidence towards a direct association between WASH practices and children’s nutritional status, suggesting the need to align nutrition and WASH programmes.
Alcohol Consumption Reduces the Beneficial Influence of Protein Intake on Muscle Mass in Middle-Aged Korean Adults: A 12-Year Community-Based Prospective Cohort Study
Authors: So Eunjin , Joung Hyojee
Published: September 2019

The influence of alcohol consumption on the association of protein intake with muscle mass was assessed using data from the Korean Genome and Epidemiology Study. Dietary protein intakes of 4412 middle-aged participants with normal baseline muscle mass were assessed using a semi-quantitative Food Frequency Questionnaire, and baseline alcohol consumption data (e.g., frequency and amount) were collected using a structured questionnaire. The skeletal muscle mass index (SMI), defined as the weight-adjusted skeletal muscle mass, was measured using multi-frequency bioelectrical impedance analyses every 2 years until the study endpoint. Low muscle mass was defined as a SMI <2 standard deviations below the sex-specific normal mean for a young reference group. During a 12-year follow-up, 395 subjects developed a low SMI. After multivariate adjustments, high protein intake (≥1.2 g/kg body weight (BW)) was shown to reduce the risk of low SMI development in both men (hazard ratio (HR): 0.24; 95% confidence interval (CI): 0.12, 0.51; p for trend < 0.001) and women (HR: 0.29; 95% CI: 0.16, 0.53; p for trend < 0.001), compared with low protein intake (<0.8 g/kg BW). Alcohol consumption attenuated the protective influence of protein intake against low SMI development in women (HR: 0.64; 95% CI: 0.18, 2.25; p for trend = 0.478). Among the total subjects, heavy drinkers with high protein intake were not significantly associated with the development of a low SMI (HR: 0.20; 95% CI: 0.03, 1.50; p = 0.117). Additional research should clarify the dose-response effects of alcohol consumption on muscle mass relative to daily protein intake.
Dietary Curcumin: Correlation between Bioavailability and Health Potential
Authors: Dei Cas Michele , Ghidoni Riccardo
Published: September 2019

The yellow pigment curcumin, extracted from turmeric, is a renowned polyphenol with a broad spectrum of health properties such as antioxidant, anti-inflammatory, anti-cancer, antidiabetic, hepatoprotective, anti-allergic, anti-dermatophyte, and neuroprotective. However, these properties are followed by a poor pharmacokinetic profile which compromises its therapeutic potential. The association of low absorption by the small intestine and the extensive reductive and conjugative metabolism in the liver dramatically weakens the oral bioavailability. Several strategies such as inhibition of curcumin metabolism with adjuvants as well as novel solid and liquid oral delivery systems have been tried to counteract curcumin poor absorption and rapid elimination from the body. Some of these drug deliveries can successfully enhance the solubility, extending the residence in plasma, improving the pharmacokinetic profile and the cellular uptake.
14C-Cobalamin Absorption from Endogenously Labeled Chicken Eggs Assessed in Humans Using Accelerator Mass Spectrometry
Authors: Garrod Marjorie G. , Rossow Heidi A. , Calvert Christopher C. , Miller Joshua W. , Green Ralph , Buchholz Bruce A. , Allen Lindsay H.
Published: September 2019

Traditionally, the bioavailability of vitamin B-12 (B12) from in vivo labeled foods was determined by labeling the vitamin with radiocobalt (57Co, 58Co or 60Co). This required use of penetrating radioactivity and sometimes used higher doses of B12 than the physiological limit of B12 absorption. The aim of this study was to determine the bioavailability and absorbed B12 from chicken eggs endogenously labeled with 14C-B12 using accelerator mass spectrometry (AMS). 14C-B12 was injected intramuscularly into hens to produce eggs enriched in vivo with the 14C labeled vitamin. The eggs, which provided 1.4 to 2.6 μg of B12 (~1.1 kBq) per serving, were scrambled, cooked and fed to 10 human volunteers. Baseline and post-ingestion blood, urine and stool samples were collected over a one-week period and assessed for 14C-B12 content using AMS. Bioavailability ranged from 13.2 to 57.7% (mean 30.2 ± 16.4%). Difference among subjects was explained by dose of B12, with percent bioavailability from 2.6 μg only half that from 1.4 μg. The total amount of B12 absorbed was limited to 0.5–0.8 μg (mean 0.55 ± 0.19 μg B12) and was relatively unaffected by the amount consumed. The use of 14C-B12 offers the only currently available method for quantifying B12 absorption in humans, including food cobalamin absorption. An egg is confirmed as a good source of B12, supplying approximately 20% of the average adult daily requirement (RDA for adults = 2.4 μg/day).
Dietary Fiber and Gut Microbiota in Renal Diets
Authors: Camerotto Carla , Cupisti Adamasco , D’Alessandro Claudia , Muzio Fulvio , Gallieni Maurizio
Published: September 2019

Nutrition is crucial for the management of patients affected by chronic kidney disease (CKD) to slow down disease progression and to correct symptoms. The mainstay of the nutritional approach to renal patients is protein restriction coupled with adequate energy supply to prevent malnutrition. However, other aspects of renal diets, including fiber content, can be beneficial. This paper summarizes the latest literature on the role of different types of dietary fiber in CKD, with special attention to gut microbiota and the potential protective role of renal diets. Fibers have been identified based on aqueous solubility, but other features, such as viscosity, fermentability, and bulking effect in the colon should be considered. A proper amount of fiber should be recommended not only in the general population but also in CKD patients, to achieve an adequate composition and metabolism of gut microbiota and to reduce the risks connected with obesity, diabetes, and dyslipidemia.
The Effect of Maternal Obesity on Breast Milk Fatty Acids and Its Association with Infant Growth and Cognition—The PREOBE Follow-Up
Authors: de la Garza Puentes Andrea , Martí Alemany Adrià , Chisaguano Aida Maribel , Montes Goyanes Rosa , Castellote Ana I. , Torres-Espínola Franscisco J. , García-Valdés Luz , Escudero-Marín Mireia , Segura Maria Teresa , Campoy Cristina , López-Sabater M. Carmen
Published: September 2019

This study analyzed how maternal obesity affected fatty acids (FAs) in breast milk and their association with infant growth and cognition to raise awareness about the programming effect of maternal health and to promote a healthy prenatal weight. Mother–child pairs (n = 78) were grouped per maternal pre-pregnancy body mass index (BMI): normal-weight (BMI = 18.5–24.99), overweight (BMI = 25–29.99) and obese (BMI > 30). Colostrum and mature milk FAs were determined. Infant anthropometry at 6, 18 and 36 months of age and cognition at 18 were analyzed. Mature milk exhibited lower arachidonic acid (AA) and docosahexaenoic acid (DHA), among others, than colostrum. Breast milk of non-normal weight mothers presented increased saturated FAs and n6:n3 ratio and decreased α-linolenic acid (ALA), DHA and monounsaturated FAs. Infant BMI-for-age at 6 months of age was inversely associated with colostrum n6 (e.g., AA) and n3 (e.g., DHA) FAs and positively associated with n6:n3 ratio. Depending on the maternal weight, infant cognition was positively influenced by breast milk linoleic acid, n6 PUFAs, ALA, DHA and n3 LC-PUFAs, and negatively affected by n6:n3 ratio. In conclusion, this study shows that maternal pre-pregnancy BMI can influence breast milk FAs and infant growth and cognition, endorsing the importance of a healthy weight in future generations.
The Mediterranean Diet and Cancer: What Do Human and Molecular Studies Have to Say about It?
Authors: Hernáez Álvaro , Estruch Ramón
Published: September 2019

Mediterranean diet (MD) is a well-known healthy dietary pattern, linked to: (1) high intakes of olive oil as main the culinary fat, plant-based foods (fruits, vegetables, legumes, whole grains, tree nuts, and seeds), and fish; and (2) a moderate consumption of white meat, eggs, dairy products such as yogurt and cheese, and wine always with meals [...]
Perioperative Vitamin C and E levels in Cardiac Surgery Patients and Their Clinical Significance
Authors: Hill Aileen , Borgs Christina , Fitzner Christina , Stoppe Christian
Published: September 2019

Background: Oxidative stress contributes to organ dysfunction after cardiac surgery and still represents a major problem. Antioxidants, such as vitamins C and E might be organ protective. Methods: The primary objective of this prospective observational study was the description to evaluate the perioperative vitamin C and E levels in 56 patients undergoing cardiac surgery with the use of cardiopulmonary bypass. The association of vitamin C with inflammatory reaction, oxidative stress, organ dysfunctions, and clinical outcomes were evaluated in an explorative approach. Results: Vitamin C levels decreased significantly from 6.5 (3.5–11.5) mg/L before surgery to 2.8 (2.0–3.9) mg/L 48 h after surgery (p < 0.0001). Fifty-six percent of patients had a suboptimal vitamin C status even before surgery. In protein-denaturized probes, significantly higher vitamin C concentrations were detected (p = 0.0008). Vitamin E levels decreased significantly from preoperative level 11.6 (9.5–13.2) mg/L to 7.1 (5.5–7.4) mg/L, (p = 0.0002) at the end of cardiopulmonary bypass, remained low during the first day on ICU and recovered to 8.2 (7.1–9.3) mg/L 48 h after surgery. No patient was vitamin E deficient before surgery. Analysis showed no statistically significant association of vitamin C with inflammation, oxidative stress or organ dysfunction levels in patients with previously suboptimal vitamin C status or patients with a perioperative decrease of ≥50% vitamin C after surgery. Patients with higher vitamin C levels had a shorter ICU stay than those who were vitamin C depleted, which was not statistically significant (72 versus 135 h, p = 0.1990). Conclusion: Vitamin C and E levels significantly declined intraoperatively and remained significantly reduced low for 2 days after cardiac surgery. The influence of reduced serum levels on the inflammatory reaction and clinical outcome of the patients remain unclear in this small observational study and need to be investigated further. Given vitamin C´s pleiotropic role in the human defense mechanisms, further trials are encouraged to evaluate the clinical significance of Vitamin C in cardiac surgery patients.
Vitamin D and ω-3 Supplementations in Mediterranean Diet During the 1st Year of Overt Type 1 Diabetes: A Cohort Study
Authors: Cadario Francesco , Pozzi Erica , Rizzollo Stefano , Stracuzzi Marta , Beux Sheila , Giorgis Alberto , Carrera Deborah , Fullin Fabiola , Riso Sergio , Rizzo Angela Maria , Montorfano Gigliola , Bagnati Marco , Dianzani Umberto , Caimmi Philippe , Bona Gianni , Ricordi Camillo
Published: September 2019

Vitamin D and omega 3 fatty acid (ω-3) co-supplementation potentially improves type 1 diabetes (T1D) by attenuating autoimmunity and counteracting inflammation. This cohort study, preliminary to a randomized control trial (RCT), is aimed at evaluating, in a series of T1D children assuming Mediterranean diet and an intake of cholecalciferol of 1000U/day from T1D onset, if ω-3 co-supplementation preserves the residual endogen insulin secretion (REIS). Therefore, the cohort of 22 “new onsets” of 2017 received ω-3 (eicosapentenoic acid (EPA) plus docosahexaenoic acid (DHA), 60 mg/kg/day), and were compared retrospectively vs. the 37 “previous onsets” without ω-3 supplementation. Glicosilated hemoglobin (HbA1c%), the daily insulin demand (IU/Kg/day) and IDAA1c, a composite index (calculated as IU/Kg/day × 4 + HbA1c%), as surrogates of REIS, were evaluated at recruitment (T0) and 12 months later (T12). In the ω-3 supplemented group, dietary intakes were evaluated at T0 and T12. As an outcome, a decreased insulin demand (p < 0.01), particularly as pre-meal boluses (p < 0.01), and IDAA1c (p < 0.01), were found in the ω-3 supplemented group, while HbA1c% was not significantly different. Diet analysis in the ω-3 supplemented group, at T12 vs. T0, highlighted that the intake of arachidonic acid (AA) decreased (p < 0.01). At T0, the AA intake was inversely correlated with HbA1c% (p < 0.05; r;. 0.411). In conclusion, the results suggest that vitamin D plus ω-3 co-supplementation as well as AA reduction in the Mediterranean diet display benefits for T1D children at onset and deserve further investigation.
Correction: Tugault-Lafleur C.N. and Black J.L. “Differences in the Quantity and Types of Foods and Beverages Consumed by Canadians between 2004 and 2015” Nutrients 2019, 11, 526
Authors: Tugault-Lafleur Claire N. , Black Jennifer L.
Published: September 2019

We would like to submit the following corrections to our recently published paper [...]
The Dietary Management of Patients with Irritable Bowel Syndrome: A Narrative Review of the Existing and Emerging Evidence
Authors: Algera Joost , Colomier Esther , Simrén Magnus
Published: September 2019

Even though irritable bowel syndrome (IBS) has been known for more than 150 years, it still remains one of the research challenges of the 21st century. According to the current diagnostic Rome IV criteria, IBS is characterized by abdominal pain associated with defecation and/or a change in bowel habit, in the absence of detectable organic causes. Symptoms interfere with the daily life of patients, reduce health-related quality of life and lower the work productivity. Despite the high prevalence of approximately 10%, its pathophysiology is only partly understood and seems multifactorial. However, many patients report symptoms to be meal-related and certain ingested foods may generate an exaggerated gastrointestinal response. Patients tend to avoid and even exclude certain food products to relieve their symptoms, which could affect nutritional quality. We performed a narrative paper review of the existing and emerging evidence regarding dietary management of IBS patients, with the aim to enhance our understanding of how to move towards an individualized dietary approach for IBS patients in the near future.
Antibacterial Activity of Commercial Phytochemicals against Aeromonas Species Isolated from Fish
Authors: Kot Barbara , Kwiatek Kinga , Janiuk Justyna , Witeska Małgorzata , Pękala-Safińska Agnieszka
Published: September 2019

Antimicrobial activities of phytochemicals—trans-cinnamaldehyde (TC), ferulic acid (FA), p-coumaric acid (p-CA), caffeic acid (CA), chlorogenic acid (CHA), Thymus vulgaris essential oil (TO), Eugenia caryophyllus essential oil (ECO), and Melaleuca alternifolia oil (TTO) against Aeromonas species—were assessed. Growth of all Aeromonas salmonicida subsp. salmonicida and almost all Aeromonas sobria strains was inhibited by TC at concentration 0.01 mg/mL, and for most Aeromonas hydrophila strains minimal inhibitory concentrations (MIC) ranged from 0.01 to 0.19 mg/mL. The inhibitory effect of TC against A. salmonicida subsp. salmonicida was comparable to the effect of oxytetracycline, and in the case of A. salmonicida subsp. salmonicida and A. sobria was higher compared to gentamicin. MIC of FA, p-CA, and CA for most strains ranged from 1.56 to 3.12 mg/mL, and MIC values of TO for most strains ranged from 0.39 to 0.78 mg/mL. TO and TC at the concentrations below ½ MIC values used in mixtures exhibited strong synergism. ECO and TC showed synergy in mixture of ⅛ MIC of ECO and ¼ MIC of TC. TC and TO exhibited the strongest inhibitory and bactericidal effect against investigated Aeromonas species, and they are a promising alternative to the use of antibiotics in controlling the growth of these fish pathogens.
Metal–Organic Framework-Based Chemo-Photothermal Combinational System for Precise, Rapid, and Efficient Antibacterial Therapeutics
Authors: Wu Biyuan , Fu Jintao , Zhou Yixian , Shi Yin , Wang Jing , Feng Xiaoqian , Zhao Yiting , Zhou Guiling , Lu Chao , Quan Guilan , Pan Xin , Wu Chuanbin
Published: September 2019

Rapid increase of antimicrobial resistance has become an urgent threat to global public health. In this research, since photothermal therapy is a potential antibacterial strategy, which is less likely to cause resistance, a metal–organic framework-based chemo-photothermal combinational system was constructed. Zeolitic imidazolate frameworks-8 (ZIF-8), a porous carrier with unique features such as high loading and pH-sensitive degradation, was synthesized, and then encapsulated photothermal agent indocyanine green (ICG). First, ICG with improved stability in ZIF-8 (ZIF-8-ICG) can effectively produce heat in response to NIR laser irradiation for precise, rapid, and efficient photothermal bacterial ablation. Meanwhile, Zn2+ ions released from ZIF-8 can inhibit bacterial growth by increasing the permeability of bacterial cell membrane and further strengthen photothermal therapy efficacy by reducing the heat resistance of bacteria. Study showed that bacteria suffered from significant changes in morphology after treatment with ZIF-8-ICG under laser irradiation. The combinational chemo-hyperthermia therapy of ZIF-8-ICG could thoroughly ablate murine subcutaneous abscess induced by methicillin-resistant Staphylococcus aureus (MRSA), exhibiting a nearly 100% bactericidal ratio. Both in vitro and in vivo safety evaluation confirmed that ZIF-8-ICG was low toxic. Overall, our researches demonstrated that ZIF-8-ICG has great potential to be served as an alternative to antibiotics in combating multidrug-resistant bacterial pathogens.
Development and Characterization of the Solvent-Assisted Active Loading Technology (SALT) for Liposomal Loading of Poorly Water-Soluble Compounds
Authors: Pauli Griffin , Tang Wei-Lun , Li Shyh-Dar
Published: September 2019

A large proportion of pharmaceutical compounds exhibit poor water solubility, impacting their delivery. These compounds can be passively encapsulated in the lipid bilayer of liposomes to improve their water solubility, but the loading capacity and stability are poor, leading to burst drug leakage. The solvent-assisted active loading technology (SALT) was developed to promote active loading of poorly soluble drugs in the liposomal core to improve the encapsulation efficiency and formulation stability. By adding a small volume (~5 vol%) of a water miscible solvent to the liposomal loading mixture, we achieved complete, rapid loading of a range of poorly soluble compounds and attained a high drug-to-lipid ratio with stable drug retention. This led to improvements in the circulation half-life, tolerability, and efficacy profiles. In this mini-review, we summarize our results from three studies demonstrating that SALT is a robust and versatile platform to improve active loading of poorly water-soluble compounds. We have validated SALT as a tool for improving drug solubility, liposomal loading efficiency and retention, stability, palatability, and pharmacokinetics (PK), while retaining the ability of the compounds to exert pharmacological effects.
Regulation of Symbiotic Nitrogen Fixation in Legume Root Nodules
Authors: Schwember Andrés R. , Schulze Joachim , del Pozo Alejandro , Cabeza Ricardo A.
Published: September 2019

In most legume nodules, the di-nitrogen (N2)-fixing rhizobia are present as organelle-like structures inside their root host cells. Many processes operate and interact within the symbiotic relationship between plants and nodules, including nitrogen (N)/carbon (C) metabolisms, oxygen flow through nodules, oxidative stress, and phosphorous (P) levels. These processes, which influence the regulation of N2 fixation and are finely tuned on a whole-plant basis, are extensively reviewed in this paper. The carbonic anhydrase (CA)-phosphoenolpyruvate carboxylase (PEPC)-malate dehydrogenase (MDH) is a key pathway inside nodules involved in this regulation, and malate seems to play a crucial role in many aspects of symbiotic N2 fixation control. How legumes specifically sense N-status and how this stimulates all of the regulatory factors are key issues for understanding N2 fixation regulation on a whole-plant basis. This must be thoroughly studied in the future since there is no unifying theory that explains all of the aspects involved in regulating N2 fixation rates to date. Finally, high-throughput functional genomics and molecular tools (i.e., miRNAs) are currently very valuable for the identification of many regulatory elements that are good candidates for accurately dissecting the particular N2 fixation control mechanisms associated with physiological responses to abiotic stresses. In combination with existing information, utilizing these abundant genetic molecular tools will enable us to identify the specific mechanisms underlying the regulation of N2 fixation.
Phosphorus and Nitrogen Modulate Plant Performance in Shrubby Legumes from the Iberian Peninsula
Authors: Pérez-Fernández María , Míguez-Montero Ángel , Valentine Alexandre
Published: September 2019

We investigated the impact of phosphorus nutrition on plant growth and biological nitrogen fixation in four leguminous plants in the Tribe Genistea. The main objective of the study was to analyze Phosphorus and Nitrogen use efficiency under drought. We also tested for the effects of rhizobial inoculation on plant performance. Plants inoculated with Rhizobium strains isolated from plants of the four species growing in the wild were cropped under controlled conditions in soils with either low P (5 µM) or high P (500 µM). The experiment was replicated in the presence and absence of plant irrigation to test for the effects of drought stress of inoculated and non-inoculated plants under the two P levels of fertilization. Low-P treatments increased nodule production while plant biomass and shoot and root P and N contents where maximum at high P. Low P (5 µM) in the growing media, resulted in greater N accumulated in plants, coupled with greater phosphorus and nitrogen uptake efficiencies. Drought reduced the relative growth rate over two orders of magnitude or more, depending on the combination of plant species and treatment. Genista cinerea had the lowest tolerance to water scarcity, whereas Genista florida and Retama sphaerocarpa were the most resistant species to drought. Drought resistance was enhanced in the inoculated plants. In the four species, and particularly in Echinospartum barnadesii, the inoculation treatment clearly triggered N use efficiency, whereas P use efficiency was greater in the non-inoculated irrigated plants. Nodulation significantly increased in plants in the low P treatments, where plants showed a greater demand for N. The physiological basis for the four species being able to maintain their growth at low P levels and to respond to the greater P supply, is through balanced acquisition of P and N to meet the plants’ nutritional needs.
SWEET Gene Family in Medicago truncatula: Genome-Wide Identification, Expression and Substrate Specificity Analysis
Authors: Hu Bin , Wu Hao , Huang Weifeng , Song Jianbo , Zhou Yong , Lin Yongjun
Published: September 2019

SWEET (Sugars Will Eventually be Exported Transporter) proteins mediate the translocation of sugars across cell membranes and play crucial roles in plant growth and development as well as stress responses. In this study, a total of 25 SWEET genes were identified from the Medicago truncatula genome and were divided into four clades based on the phylogenetic analysis. The MtSWEET genes are distributed unevenly on the M. truncatula chromosomes, and eight and 12 MtSWEET genes are segmentally and tandemly duplicated, respectively. Most MtSWEET genes contain five introns and encode proteins with seven transmembrane helices (TMHs). Besides, nearly all MtSWEET proteins have relatively conserved membrane domains, and contain conserved active sites. Analysis of microarray data showed that some MtSWEET genes are specifically expressed in disparate developmental stages or tissues, such as flowers, developing seeds and nodules. RNA-seq and qRT-PCR expression analysis indicated that many MtSWEET genes are responsive to various abiotic stresses such as cold, drought, and salt treatments. Functional analysis of six selected MtSWEETs in yeast revealed that they possess diverse transport activities for sucrose, fructose, glucose, galactose, and mannose. These results provide new insights into the characteristics of the MtSWEET genes, which lay a solid foundation for further investigating their functional roles in the developmental processes and stress responses of M. truncatula.
Jasmonates—the Master Regulator of Rice Development, Adaptation and Defense
Authors: Trang Nguyen Hieu , Thi Mai To Huong , Lebrun Michel , Bellafiore Stephane , Champion Antony
Published: September 2019

Rice is one of the most important food crops worldwide, as well as the model plant in molecular studies on the cereals group. Many different biotic and abiotic agents often limit rice production and threaten food security. Understanding the molecular mechanism, by which the rice plant reacts and resists these constraints, is the key to improving rice production to meet the demand of an increasing population. The phytohormone jasmonic acid (JA) and related compounds, collectively called jasmonates, are key regulators in plant growth and development. They are also one of the central players in plant immunity against biotic attacks and adaptation to unfavorable environmental conditions. Here, we review the most recent knowledge about jasmonates signaling in the rice crop model. We highlight the functions of jasmonates signaling in many adaptive responses, and also in rice growth and development processes. We also draw special attention to different signaling modules that are controlled by jasmonates in rice.
Light- and Neutron-Optical Properties of Holographic Transmission Gratings from Polymer-Ionic Liquid Composites with Submicron Grating Spacing
Authors: Flauger Peter , Ellabban Mostafa A. , Glavan Gašper , Klepp Jürgen , Pruner Christian , Jenke Tobias , Geltenbort Peter , Fally Martin
Published: September 2019

We investigate the applicability of polymer-ionic liquid composites as optical elements for light, as well as for slow neutrons. The gratings are recorded using two-beam mixing and are characterized experimentally based on their diffraction properties. We produced a set of samples differing in their thickness, ranging from 10 μm–100 μm. We demonstrate that it is possible to prepare transmission gratings with a lattice constant of Λ = 480 nm, resulting in thick gratings for light, as well as neutrons. The presented samples show low optical losses in the Vis-UV spectrum and exhibit refractive index modulations of about 10 − 3 at λ = 543 nm. However, further improvements have to be made to obtain efficient neutron optical components.
Enhanced Poly(propylene carbonate) with Thermoplastic Networks: A Cross-Linking Role of Maleic Anhydride Oligomer in CO2/PO Copolymerization
Authors: Gao Lijun , Huang Meiying , Wu Qifeng , Wan Xiaodan , Chen Xiaodi , Wei Xinxin , Yang Wenjing , Deng Rule , Wang Lingyun , Feng Jiuying
Published: September 2019

Cross-linking is an effective way to enhance biodegradable poly(propylene carbonate) (PPC) from CO2 and propylene oxide (PO). Cross-linked PPC can be prepared by one-step terpolymerization of multifunctional third monomers with CO2 and PO. However, few such third monomers are available. Each molecule of maleic anhydride oligomer (MAO) contains more than two cyclic anhydride groups. Here, we use it to synthesize PPC with cross-linked networks by adding a small quantity of MAO (0.625–5 wt% of PO) in CO2/PO copolymerization that was catalyzed by zinc glutarate. The formation of networks in the prepared copolymers was confirmed by the presence of gel in copolymers combined Fourier transform infrared spectroscopy (FT-IR), 1H NMR, and the improved mechanical properties. The 5% weight-loss degradation temperatures and maximum weight-loss degradation temperatures greatly increase up to 289.8 °C and 308.8 °C, respectively, which are remarkably high when compared to those of PPC. The minimum permanent deformation of the copolymers closes to 0, while that of PPC is 173%. The maximum tensile strength of the copolymers is 25.5 MPa higher than that of PPC, reaching 38.4 MPa, and it still has some toughness with the elongation at break of 25%. The above phenomena indicate that MAO that was inserted in PPC chains play a cross-linking role, which results in enhanced thermal stability, dimensional stability, and mechanical strength, comprehensively.
Physical Foaming and Crosslinking of Polyethylene with Modified Talcum
Authors: Kaltenegger-Uray Anna , Rieß Gisbert , Lucyshyn Thomas , Holzer Clemens , Kern Wolfgang
Published: September 2019

The aim of this study was the investigation of the use of modified talcum for supporting crosslinking and as novel nucleating agent for physical foaming of polyethylene. For the modification of the talcum, a thermal initiator was linked to the talcum surface. During the extrusion process, the initiator decomposes, and gas and radicals are formed. The gas generates the nucleation of cells and the radicals support the crosslinking process between the polymer chains. The modification of the talcum was performed in three steps: The first step was the grafting of alkoxysilanes onto the talcum surface. The second step was the chlorination of the thermal initiator for an easier linkage, and the last step was the linking between the initiator and the silanes grafted onto the talcum surface. For this study, two investigations were carried out. One investigation was the analysis of the crosslinking effect with the modified talcum. For this purpose, polyethylene plates were compression molded and the viscoelastic properties were measured with a parallel plate rheometer. The use of the modified talcum led to a higher crosslinking density. The second investigation was the physical foaming experiment in an extrusion process with nitrogen as blowing agent using both a pure and the modified talcum as nucleating agents. The foamed samples were characterized in terms of density, cell size and cell density, and compared with each other. The blend with the modified nucleating agent indicated a foam structure with a smaller mean cell size and a lower density compared to the use of the pristine nucleating agent.
Phase Diagrams of n-Type Low Bandgap Naphthalenediimide-Bithiophene Copolymer Solutions and Blends
Authors: Fanta Gada Muleta , Jarka Pawel , Szeluga Urszula , Tański Tomasz , Kim Jung Yong
Published: September 2019

Phase diagrams of n-type low bandgap poly{(N,N′-bis(2-octyldodecyl)naphthalene -1,4,5,8-bis(dicarboximide)-2,6-diyl)-alt-5,5′,-(2,2′-bithiophene)} (P(NDI2OD-T2)) solutions and blends were constructed. To this end, we employed the Flory–Huggins (FH) lattice theory for qualitatively understanding the phase behavior of P(NDI2OD-T2) solutions as a function of solvent, chlorobenzene, chloroform, and p-xylene. Herein, the polymer–solvent interaction parameter (χ) was obtained from a water contact angle measurement, leading to the solubility parameter. The phase behavior of these P(NDI2OD-T2) solutions showed both liquid–liquid (L–L) and liquid–solid (L–S) phase transitions. However, depending on the solvent, the relative position of the liquid–liquid phase equilibria (LLE) and solid–liquid phase equilibria (SLE) (i.e., two-phase co-existence curves) could be changed drastically, i.e., LLE > SLE, LLE ≈ SLE, and SLE > LLE. Finally, we studied the phase behavior of the polymer–polymer mixture composed of P(NDI2OD-T2) and regioregular poly(3-hexylthiophene-2,5-dyil) (r-reg P3HT), in which the melting transition curve was compared with the theory of melting point depression combined with the FH model. The FH theory describes excellently the melting temperature of the r-reg P3HT/P(NDI2OD-T2) mixture when the entropic contribution to the polymer–polymer interaction parameter (χ = 116.8 K/T − 0.185, dimensionless) was properly accounted for, indicating an increase of entropy by forming a new contact between two different polymer segments. Understanding the phase behavior of the polymer solutions and blends affecting morphologies plays an integral role towards developing polymer optoelectronic devices.
Fluorescent and Mechanical Properties of Silicon Quantum Dots Modified Sodium Alginate-Carboxymethylcellulose Sodium Nanocomposite Bio-Polymer Films
Authors: Ji Yali , Zhang Huimin , Zhang Canfu , Quan Zhiyi , Huang Min , Wang Lili
Published: September 2019

Highly luminescent silicon quantum dots (SiQDs) were prepared via one-pot hydrothermal route. Furthermore, the optimal synthetic conditions, dependence of the emission spectrum on the excitation wavelength and fluorescent stability of SiQDs were investigated by fluorescence spectroscopy. SiQDs exhibited bright blue fluorescence, and photoluminescence (PL) lifetime is 10.8 ns when excited at 325 nm. The small-sized SiQDs (~3.3 nm) possessed uniform particle size, crystal lattice spacing of 0.31 nm and silicon (111), (220) crystal planes. Luminescent SiQDs/sodium alginate (SA)-carboxymethylcellulose sodium (CMC) nanocomposite bio-polymer films were successfully fabricated by incorporating SiQDs into the SA-CMC matrix. Meanwhile, SiQDs not only impart strong fluorescence to the polymer, but also make the composite films have favorable toughness.
Go and Sin No More: The Afterlife as Moral Teaching in Italian Catholic Educational Theatre
Authors: Cavallaro Daniela
Published: September 2019

Catholic religious orders that have education as part of their mission have often used visions of the afterlife in theatre productions as vehicles to transmit a message of conversion, especially to those who, because of age or illiteracy, would not benefit as much from Scripture readings or complex sermons. In this article, I look at how such visions of the blessed and the damned, of heaven and hell, of angels and demons, were used in educational theatre in Italy by the Jesuits in the 16th century and the Salesian sisters in the 20th century. The historical background for the Jesuit and Salesian plays I analyze also reveals a propagandistic layer of meaning in their representation of the afterworld, as the Jesuits’ tragedies date to the years of the Counter-reformation, while the Salesian sisters’ plays belong to era of the cold war. Thus, the Jesuit and Salesian theatrical depictions of heaven and hell provide insight not only into the religious understanding of the eras, but also into the social and political concerns of the times in which they were composed, as well as the diverse educational messages transmitted to young men and young women.
Cults, Crosses, and Crescents: Religion and Healing from Colonial Violence in Tanzania
Authors: Rushohora Nancy , Silayo Valence
Published: September 2019

More often than not, Africans employed local religion and the seemingly antagonistic faith of Christianity and Islam, to respond to colonial exploitation, cruelty, and violence. Southern Tanzanians’ reaction during the Majimaji resistance presents a case in point where the application of local religion, Christianity, and Islam for both individual and community spiritual solace were vivid. Kinjekitile Ngwale—the prominent war ritualist—prophesied that a concoction (Maji) would turn the German’s bullets to water, which in turn would be the defeat of the colonial government. Equally, Christian and Islamic doctrines were used to motivate the resistance. How religion is used in the post-colonial context as a cure for maladies of early 20th-century colonialism and how local religion can inspire political change is the focus of this paper. The paper suggests that religion, as propagated by the Majimaji people for the restoration of social justice to the descendant’s communities, is a form of cultural heritage playing a social role of remedying colonial violence.
Improving Water Access and Health through Rainwater Harvesting: Perceptions of an Indigenous Community in Jalisco, Mexico
Authors: González-Padrón Shiara K. , Lerner Amy M. , Mazari-Hiriart Marisa
Published: September 2019

This article presents a mixed methods case study of an isolated indigenous community (the Wixaritari) in Jalisco state, Mexico, where the patterns of access to water have been changing due to Rainwater Harvesting Systems (RWHSs). The lack of water in this region is extremely precarious; women and children spend approximately one to three hours a day fetching water, and families live with less than 20 L per person/day. To better understand the relationship between water access and health derived from the use of RWHSs, we first analyzed epidemiological data (2014–2017) from cases of Acute Diarrheal Diseases (ADDs) and household water quality. Next, we surveyed households on water access after the introduction of RWHSs, including questions about perceptions of health problems associated with water. The results revealed that the introduction of RWHSs water access in this community has increased quantitatively and qualitatively and the incidence of ADDs has decreased significantly. However, the surveys and interviews indicate a disconnect between the incidence of ADDs and perceptions of health problems. We conclude that RWHSs can have tangible benefits, but they can be potentially undermined by perceptions of communities that have different understandings of the cause of disease.
Case Studies for Dangerous Dust Explosions in South Korea during Recent Years
Authors: Pak Seonggyu , Jung Seongho , Roh Changhyun , Kang Chankyu
Published: September 2019

Despite recent extensive research and technical development to prevent and mitigate dust explosions, processes that produce and handle combustible materials in the form of powders and dusts, either as a main product or as an undesired by-product, have become a constant dust explosion threat as they become more sophisticated and complicated. This study analyzed the characteristics of 53 dust explosions that occurred in South Korea over the last 30 years, and investigated the differences of dust explosions that happened in various countries, such as Japan, the United States, the United Kingdom, and France. In addition, case studies showed the severity of dust explosions occurring in South Korea. Through the special focus on the three most recent years of dust explosions, the causes and processes of the accidents were identified. Analyses of dust explosions in South Korea show that they were mainly caused by organic matter and metal, and, unfortunately, dust explosions occurred repeatedly during grinding, mixing, and injection of powder materials into facilities. No reported accidents occurred during the production processes of wood or paper during the last three years. Taking these characteristics into account, effective ways to prevent or mitigate dust explosions at workplaces where many dust explosions occurred were suggested.
Sustainability Initiatives in Emerging Economies: A Socio-Cultural Perspective
Authors: Malik Mohsin , Abdallah Salam
Published: September 2019

This paper explains how and why sustainable supply chain management (SSCM) is different in an emerging economy by investigating sustainability initiatives in the United Arab Emirates. This exploratory qualitative study uses activity theory (AT) as a framework of analyses to investigate the sustainable supply chain practices in the context of emerging economies. AT’s hierarchical breakdown structure allows complex and dynamic activities to be broken down across multiple levels providing a simplified explanation of the triple bottom line initiatives across 16 participant organizations. Our findings corroborate with some of the sustainability initiatives in developed countries, such as the contribution of suppliers and the importance of employee engagement to the sustainable efforts of the focal firm; however, there were significant differences too. Customer and investor pressure was the top driver toward sustainability in developed economies, but our research identifies government pressure as the principal motive for sustainability. Similarly, we observe a narrow perspective on sustainability from the studied organizations as sustainability measures were seen as “costly”. We attribute this to a lack of accounting for the costs associated with being “unsustainable”. We contribute to the literature by developing four propositions that identify the key contributors to successful sustainability outcomes in the context of emerging economies: (a) the dynamic influence of governments on the adoption and implementation of SSCM; (b) accounting for the costs associated with the “unsustainable” measures, which enables a strategic perspective on sustainability; (c) the inclusion of sustainability as an “order qualifier” for the suppliers; and (d) the employees’ transition from compliance to taking ownership of sustainability initiatives.