bluuebunny/crossref_metadata_2025 huggingface dataset:
Systemic Metabolic and Volumetric Assessment via Whole-Body [18F]FDG-PET/CT: Pancreas Size Predicts Cachexia in Head and Neck Squamous Cell Carcinoma
Authors: Yu Josef , Spielvogel Clemens , Haberl David , Jiang Zewen , Özer Öykü , Pusitz Smilla , Geist Barbara , Beyerlein Michael , Tibu Iustin , Yildiz Erdem , Kandathil Sam Augustine , Buschhorn Till , Schnöll Julia , Kumpf Katarina , Chen Ying-Ting , Wu Tingting , Zhang Zhaoqi , Grünert Stefan , Hacker Marcus , Vraka Chrysoula
Published: September 2024

Background/Objectives: Cancer-associated cachexia in head and neck squamous cell carcinoma (HNSCC) is challenging to diagnose due to its complex pathophysiology. This study aimed to identify metabolic biomarkers linked to cachexia and survival in HNSCC patients using [18F]FDG-PET/CT imaging and machine learning (ML) techniques. Methods: We retrospectively analyzed 253 HNSCC patients from Vienna General Hospital and the MD Anderson Cancer Center. Automated organ segmentation was employed to quantify metabolic and volumetric data from [18F]FDG-PET/CT scans across 29 tissues and organs. Patients were categorized into low weight loss (LoWL; grades 0–2) and high weight loss (HiWL; grades 3–4) groups, according to the weight loss grading system (WLGS). Machine learning models, combined with Cox regression, were used to identify survival predictors. Shapley additive explanation (SHAP) analysis was conducted to determine the significance of individual features. Results: The HiWL group exhibited increased glucose metabolism in skeletal muscle and adipose tissue (p = 0.01), while the LoWL group showed higher lung metabolism. The one-year survival rate was 84.1% in the LoWL group compared to 69.2% in the HiWL group (p < 0.01). Pancreatic volume emerged as a key biomarker associated with cachexia, with the ML model achieving an AUC of 0.79 (95% CI: 0.77–0.80) and an accuracy of 0.82 (95% CI: 0.81–0.83). Multivariate Cox regression confirmed pancreatic volume as an independent prognostic factor (HR: 0.66, 95% CI: 0.46–0.95; p < 0.05). Conclusions: The integration of metabolic and volumetric data provided a strong predictive model, highlighting pancreatic volume as a key imaging biomarker in the metabolic assessment of cachexia in HNSCC. This finding enhances our understanding and may improve prognostic evaluations and therapeutic strategies.
Regulation of Enterocyte Brush Border Membrane Primary Na-Absorptive Transporters in Human Intestinal Organoid-Derived Monolayers
Authors: Haynes Jennifer , Palaniappan Balasubramanian , Crutchley John M. , Sundaram Uma
Published: September 2024

In the small intestine, sodium (Na) absorption occurs primarily via two apical transporters, Na-hydrogen exchanger 3 (NHE3) and Na-glucose cotransporter 1 (SGLT1). The two primary Na-absorptive pathways were previously shown to compensatorily regulate each other in rabbit and rat intestinal epithelial cells. However, whether NHE3 and SGLT1 regulate one another in normal human enterocytes is unknown, mainly due to a lack of appropriate experimental models. To investigate this, we generated 2D enterocyte monolayers from human jejunal 3D organoids and used small interfering RNAs (siRNAs) to knock down NHE3 or SGLT1. Molecular and uptake studies were performed to determine the effects on NHE3 and SGLT1 expression and activity. Knockdown of NHE3 by siRNA in enterocyte monolayers was verified by qPCR and Western blot analysis and resulted in reduced NHE3 activity. However, in NHE3 siRNA-transfected cells, SGLT1 activity was significantly increased. siRNA knockdown of SGLT1 was confirmed by qPCR and Western blot analysis and resulted in reduced SGLT1 activity. However, in SGLT1 siRNA-transfected cells, NHE3 activity was significantly increased. These results demonstrate for the first time the functionality of siRNA in patient-derived organoid monolayers. Furthermore, they show that the two primary Na absorptive pathways in human enterocytes reciprocally regulate one another.
β-Amyloids and Immune Responses Associated with Alzheimer’s Disease
Authors: Kolobova Elizaveta , Petrushanko Irina , Mitkevich Vladimir , Makarov Alexander A , Grigorova Irina L
Published: September 2024

Alzheimer’s disease (AD) is associated with the accumulation of β-amyloids (Aβs) and the formation of Aβ plaques in the brain. Various structural forms and isoforms of Aβs that have variable propensities for oligomerization and toxicity and may differentially affect the development of AD have been identified. In addition, there is evidence that β-amyloids are engaged in complex interactions with the innate and adaptive immune systems, both of which may also play a role in the regulation of AD onset and progression. In this review, we discuss what is currently known about the intricate interplay between β-amyloids and the immune response to Aβs with a more in-depth focus on the possible roles of B cells in the pathogenesis of AD.
An Anti-Invasive Role for Mdmx through the RhoA GTPase under the Control of the NEDD8 Pathway
Authors: Bou Malhab Lara J. , Schmidt Susanne , Fagotto-Kaufmann Christine , Pion Emmanuelle , Gadea Gilles , Roux Pierre , Fagotto Francois , Debant Anne , Xirodimas Dimitris P.
Published: September 2024

Mdmx (Mdm4) is established as an oncogene mainly through repression of the p53 tumour suppressor. On the other hand, anti-oncogenic functions for Mdmx have also been proposed, but the underlying regulatory pathways remain unknown. Investigations into the effect of inhibitors for the NEDD8 pathway in p53 activation, human cell morphology, and in cell motility during gastrulation in Xenopus embryos revealed an anti-invasive function of Mdmx. Through stabilisation and activation of the RhoA GTPase, Mdmx is required for the anti-invasive effects of NEDDylation inhibitors. Mechanistically, through its Zn finger domain, Mdmx preferentially interacts with the inactive GDP-form of RhoA. This protects RhoA from degradation and allows for RhoA targeting to the plasma membrane for its subsequent activation. The effect is transient, as prolonged NEDDylation inhibition targets Mdmx for degradation, which subsequently leads to RhoA destabilisation. Surprisingly, Mdmx degradation requires non-NEDDylated (inactive) Culin4A and the Mdm2 E3-ligase. This study reveals that Mdmx can control cell invasion through RhoA stabilisation/activation, which is potentially linked to the reported anti-oncogenic functions of Mdmx. As inhibitors of the NEDD8 pathway are in clinical trials, the status of Mdmx may be a critical determinant for the anti-tumour effects of these inhibitors.
The Interplay between Liver and Adipose Tissue in the Onset of Liver Diseases: Exploring the Role of Vitamin Deficiency
Authors: Tattoli Ivan , Mathew Aimee Rachel , Verrienti Antonella , Pallotta Lucia , Severi Carola , Andreola Fausto , Cavallucci Virve , Giorgi Mauro , Massimi Mara , Bencini Lapo , Fidaleo Marco
Published: September 2024

The deficiency of vitamins, a condition known as “hidden hunger”, causes comprehensive pathological states. Research over the years has identified a relationship between liver diseases and hypovitaminosis or defects in vitamin metabolism. The exact mechanisms remain elusive; however, the crucial involvement of specific vitamins in metabolic functions, alongside the reclassification of liver disease as metabolic dysfunction-associated steatotic liver disease (MASLD), has prompted researchers to investigate the potential cause-effect dynamics between vitamin deficiency and liver disease. Moreover, scientists are increasingly investigating how the deficiency of vitamins might disrupt specific organ crosstalk, potentially contributing to liver disease. Although the concept of a dysmetabolic circuit linking adipose tissue and the liver, leading to liver disease, has been discussed, the possible involvement of vitamin deficiency in this axis is a relatively recent area of study, with numerous critical aspects yet to be fully understood. In this review, we examine research from 2019 to July 2024 focusing on the possible link between liver-adipose tissue crosstalk and vitamin deficiency involved in the onset and progression of non-alcoholic fatty liver disease (NAFLD). Studies report that vitamin deficiency can affect the liver-adipose tissue axis, mainly affecting the regulation of systemic energy balance and inflammation.
Novel Fluorescence-Based Methods to Determine Infarct and Scar Size in Murine Models of Reperfused Myocardial Infarction
Authors: Duplessis Ashley , Elster Christin , Becher Stefanie , Engel Christina , Lang Alexander , Kaldirim Madlen , Jung Christian , Grandoch Maria , Kelm Malte , Pfeiler Susanne , Gerdes Norbert
Published: September 2024

Determination of infarct and scar size following myocardial infarction (MI) is commonly used to evaluate the efficacy of potential cardioprotective treatments in animal models. However, histological methods to determine morphological features in the infarcted heart have barely improved since implementation while still consuming large parts of the tissue and offering little options for parallel analyses. We aim to develop a new fluorescence technology for determining infarct area and area at risk that is comparable to 2,3,5-triphenyltetrazolium chloride (TTC) staining but allows for multiple analyses on the same heart tissue. For early and late time points following MI, we compared classical histochemical approaches with fluorescence staining methods. Reperfused MI was induced in male mice, the hearts were extracted 24 h, 7-, 21-, or 28-days later and fluorescently stained by combining Hoechst and phalloidin. This approach allowed for clear visualization of the infarct area, the area at ischemic risk and the remote area not affected by MI. The combined fluorescence staining correlated with the classic TTC/Evans Blue staining 24 h after MI (r = 0.8334). In later phases (>7 d) post-MI, wheat germ agglutinin (WGA) is equally accurate as classical Sirius Red (r = 0.9752), Masson’s (r = 0.9920) and Gomori’s Trichrome (r = 0.8082) staining for determination of scar size. Additionally, feasibility to co-localize fluorescence-stained immune cells in specific regions of the infarcted myocardium was demonstrated with this protocol. In conclusion, this new procedure for determination of post-MI infarct size is not inferior to classical TTC staining, yet provides substantial benefits, including the option for unbiased software-assisted analysis while sparing ample residual tissue for additional analyses. Overall, this enhances the data quality and reduces the required animal numbers consistent with the 3R concept of animal experimentation.
Alternative Balance between Transcriptional and Epigenetic Regulation during Developmental Proliferation of Human Cranial Neural Crest Cells
Authors: Desterke Christophe , Francés Raquel , Monge Claudia , Marchio Agnès , Pineau Pascal , Mata-Garrido Jorge
Published: September 2024

Cranial neural crest cells are implicated in multiple transcriptional events at the different stages of differentiation during development. The alteration of some transcription factors expressed during neural crest development, like PAX7, could be implicated in the etiology of face malformation in murine models. Epigenetic regulation has been shown to be an important mechanistic actor in the control of timing and the level of gene expression at different stages of neural crest development. During this work, we investigated the interconnection between epigenetics and transcription factors across a diversity of human development cranial neural crest cells. Across a diversity of neural cells from human developing cranial tissues, in accordance with their proliferation stage, an alternative balance of regulation between transcription factors and epigenetic factors was identified.
Comparative Effects of Stimulant and Antipsychotic Medications on Eating Behaviors and Weight in Children with Attention Deficit Hyperactivity Disorder
Authors: Aykutlu Hasan Cem , Okyar Esra , Karadağ Mehmet , Öztürk Masum
Published: September 2024

Background/Objectives: Attention-Deficit/Hyperactivity Disorder (ADHD) is associated with an increased risk of obesity and disordered eating behaviors. This study compared weight status and eating behaviors among drug-naïve ADHD children, those on stimulant monotherapy, those on combined stimulant and antipsychotic treatment, and healthy controls. Methods: This cross-sectional study included 547 children aged 6–12 years from four Turkish provinces: 361 with ADHD (152 drug-naïve, 156 on stimulants, and 53 on combined therapy), and 186 healthy controls. Anthropometric measurements, psychiatric assessments, and eating behavior evaluations were conducted using standardized tools. Results: Drug-naïve ADHD children had the highest obesity rate (13.8%), while those on stimulant monotherapy had the lowest (4.5%) compared to controls. Combined treatment group obesity rates were similar to controls (7.5% vs. 8.6%). The drug-naïve and combined treatment groups showed increased food approach behavior and desire to drink, with the combined treatment group also showing increased emotional overeating. Conclusions: This study reveals a complex relationship between ADHD, its pharmacological management, and the risk of obesity. Stimulant monotherapy may mitigate the risk of obesity, while combined stimulant and antipsychotic treatment may lead to problematic eating behaviors. These findings emphasize the importance of monitoring weight status and eating behaviors in ADHD children, especially those receiving pharmacological interventions.
Updating Clinical Practice: Improving Perioperative Pain Management for Adeno-Tonsillectomy in Children
Authors: Redondo-Enríquez Juan Manuel , Rivas-Medina María , Galán-Mateos Manuel María
Published: September 2024

Background/Objective: Perioperative acute pain management in pediatric patients is essential to reduce complications. Adenoidectomy-Tonsillectomy are surgical procedures requiring pain control, and risk minimization for postoperative bleeding, nausea, and vomiting. Despite their known secondary effects, the use of opioid analgesics is still preponderant in pediatric perioperative management. We performed a comprehensive review on adeno-tonsillectomy perioperative pain management in children. We developed and implemented a multimodal analgesia protocol aimed to improve patients’ pain management while consistently reducing opioids use. Methods/Results: relevant Information was summarized, then compared to our clinical needs. Learnings were used to create and implement a multimodal analgesia protocol that we use in patients 3–9 years-old undergoing adenoidectomy/tonsillectomy. The full protocol is presented. Analgesic strategies have emerged to reduce or avoid the use of opioids. Among these strategies, combining different non-opioid analgesics (Ibuprofen, Paracetamol, Metamizole) has been shown to be an effective and safe pharmacological strategy when implemented as part of perioperative multimodal analgesia protocols. Considerable evidence associating the use of NSAIDs with a bigger risk of postoperative bleeding does not exist. Conclusions: Perioperative management of adenotonsillectomy pain should include preventive and multimodal analgesia, which have shown to provide significantly more effective analgesia than some opioid regimens. Ibuprofen offers highly effective analgesia for postoperative pain, particularly when combined with acetaminophen.
Scientific Integrity and Transparency in Academic Writing: The Foundation of Credible Science
Authors: Wedderkopp Niels , Rutz Erich
Published: September 2024

In the academic community, discussions and debates are a natural and vital part of the research process [...]
PASS Theory and Movement Disorders: Methodology for Assessment and Intervention
Authors: Asonitou Katerina , Koutsouki Dimitra
Published: September 2024

Background/Objectives: Executive dysfunction appears to be a significant secondary characteristic frequently linked with movement disorders. Planning is a high-level cognitive process integral to our executive functions. Children who show deficits in their planning ability usually have difficulties in making decisions or problem-solving, such as initiating tasks or mentally organizing a project, and monitoring and controlling their behavior effectively. These difficulties can significantly impact their academic performance and daily functioning even in adulthood. However, the existing research on the relationships between motor performance and planning abilities is limited and inconsistent. Methods: This study aimed to explore the potential associations between motor and planning skills in 30 preschool-aged children with Developmental Coordination Disorder (DCD) and 30 healthy controls matched for age, including utilizing the PASS theory and Cognitive Assessment System (CAS). Results: The findings highlighted balance, total motor score, and planned codes as the primary factors distinguishing the two groups. A significant Pearson’s correlation was observed between the overall MABC-2 scores and the subdomain scores, along with the Planning Scale indices for both groups, suggesting a substantial relationship between these assessments. Among the Planned codes items, the most notable predictor of overall motor performance in children was identified. Furthermore, the total score for Planned Connections emerged as the most robust predictor for tasks associated with manual dexterity. Conclusions: The relationship between motor skills and executive functions in early childhood plays a vital role in the development of early intervention strategies that utilize cognitive-motor tools.
Analysis of Prevalence and Related Factors of Cyberbullying–Victimization among Adolescents
Authors: Ma Jun , Su Liyan , Li Minhui , Sheng Jiating , Liu Fangdu , Zhang Xujun , Yang Yaming , Xiao Yue
Published: September 2024

Background/Objectives: Cyberbullying is an increasingly serious issue that negatively impacts the mental and physical health of adolescents. This study aims to report the prevalence rates of adolescent cyberbullying–victimization and its associated related factors, providing a scientific basis for targeted efforts to protect the mental and physical well-being of adolescents; Methods: From March to May 2019, there were 13 high schools and 33 middle schools in Yixing, with a student ratio of 2:1 between middle and high school. Using a random cluster sampling method, we selected four high schools and three middle schools based on this ratio, resulting in a total of 13,258 students. We conducted a survey using a self-designed questionnaire to investigate the experiences of adolescents with cyberbullying and victimization, comparing the differences in cyberbullying–victimization based on various demographic characteristics. Additionally, we employed a multifactorial logistic regression model to analyze the associated factors; Results: The rate of adolescents who declared themselves as cyberbully-victims is 2.9%. The results of the logistic regression analysis indicate that being male, having both parents working outside the home, experiencing occasional or large conflicts among family members, being subjected to punishment-and-abuse child discipline, always or often using social software (websites), enjoying playing single or multiplayer games, self-smoking, and self-drinking were associated with a higher likelihood of being a cyberbully-victim (p < 0.05); Conclusions: Adolescent cyberbullying–victimization is affected by personal, family, and social factors. Therefore, comprehensive strategies and measures are needed to intervene in this problem.
Relationship between Physical Activity Levels and Academic Performance in Adolescents from Serbia
Authors: Ilić Tamara , Stojanović Stefan , Rančić Doroteja , Jorgić Bojan Milenko , Cristian Rareș Stănescu , Iordan Daniel Andrei , Mircea Codreanu Corneliu , Leonard Stoica , Onu Ilie
Published: September 2024

Background/Objectives: Today’s high school students often engage in sedentary behavior by choosing passive activities during leisure time. Increasing research shows that regular physical activity (PA) provides benefits beyond physical health, especially important during childhood and adolescence. Our study investigated the association between physical activity levels and academic performance (AP) in Serbian adolescents. Methods: We selected a sample of 836 fourth-grade high school students (avg. 17.9 ± 0.7 years old) who completed the IPAQ (short-version) and the Academic Performance Scale. Data analysis was conducted using IBM SPSS 21.0. Due to non-normal data distribution, Spearman’s correlation was used and hierarchical regression analysis to assess the association between PA and AP. Results: The findings revealed significant correlations between vigorous, moderate and light PA, as well as overall units of metabolic equivalent of task (METs), with AP (p-values: 0.000, 0.005, 0.021, 0.004, respectively), although with weak correlation coefficients (0.127, 0.098, 0.080, 0.181, respectively). Vigorous PA influenced AP by 10.1%, moderate PA by 8.2%, and light PA by 11.4%. Conclusions: These results suggest that adolescents engaging in higher PA levels tend to have better AP. This finding indicates a substantial association between PA levels and AP, with both moderate and vigorous PA showing a positive correlation and influence. Further investigation is necessary to comprehensively grasp the link between light PA and AP.
Clinical Characteristics and Current Treatment Modality of Preterm Infants with Ureaplasma spp. Infection
Authors: Zhang Zhenhai , Wang Jian , Chen Wenwen , Xu Liping
Published: September 2024

Background: The impact of and countermeasures for Ureaplasma spp. in neonates remain controversial. The aim of this study was to evaluate the associated perinatal factors that can predict the likelihood of respiratory tract Ureaplasma spp. colonization and analyze the subsequent clinical course of affected infants, thereby providing the rationale for their diagnosis, treatment, and future study. Methods: This was a retrospective observational study of infants born at a gestational age (GA) of less than 32 weeks. Results: The prevalence of respiratory tract Ureaplasma spp. colonization was 25.8% (75/291), and it increased with a decrease in GA and birth weight (BW). Maternal vaginal Ureaplasma spp. colonization increased the risk of neonatal Ureaplasma spp. colonization, with an OR of 7.8 (95% CI: 3.1, 20.0). Infants with Ureaplasma spp. colonization had a higher white blood cell (WBC) count, normal C-reactive protein (CRP) level, and higher failure rate of weaning from mechanical ventilation (30.7% vs. 17.1%, p = 0.014); they also suffered more from interstitial pneumonia (20.0% vs. 5.6%, p < 0.001) and bronchopulmonary dysplasia (36.0% vs. 13.4%, p < 0.001). Infants receiving anti-Ureaplasma spp. treatment had a lower GA, lower BW, and more severe respiratory syndromes. However, the difference in respiratory manifestation became insignificant after adjusting for GA. Conclusions: GA and maternal vaginal Ureaplasma spp. colonization could be used to predict neonatal respiratory tract Ureaplasma spp. colonization. An elevated WBC count combined with normal CRP is a good marker of Ureaplasma spp. colonization/infection. It is conventional practice to start anti-Ureaplasma spp. treatment when infants present with a deteriorated respiratory condition. This practice warrants further investigation considering GA as a predominant intermediate variable.
Contrast-Enhanced Ultrasound (CEUS) and Ultra-Microangiography (UMA) in Critically Ill Children with Acute Kidney Injury
Authors: Ogorevc Nace , Slak Peter , Nikšić Stevan , Novljan Gregor , Fister Petja , Plut Domen
Published: September 2024

Acute kidney injury (AKI) is an acute condition of impaired kidney function with decreased glomerular filtration rate, which results in dysregulation in volume, electrolyte, and acid–base equilibrium. AKI can be a life-threatening condition and can also lead to chronic kidney disease. It is important to diagnose AKI early in the course of the disease or to predict its development, as this can influence therapeutic decisions, outcome, and, consequently, the prognosis. In clinical practice, an elevated serum creatinine concentration remains the most common laboratory indicator for diagnosing AKI. However, due to the delay in its rise, creatinine levels are often insensitive and inaccurate for early diagnosis. Novel biomarkers of kidney tubular injury and the renal angina index have shown promise in predicting AKI earlier and more accurately. Contrast-enhanced ultrasonography (CEUS) and ultra-microangiography (UMA) are radiological methods that can quantify renal microperfusion and may be able to predict the development of AKI. They have not yet been used for quantifying renal perfusion in children with risk factors for developing AKI. Further research is needed to compare these sonographic techniques with the renal angina index and emerging kidney injury biomarkers for predicting acute kidney injury (AKI) in both children and adults.
Production of GcMAF with Anti-Inflammatory Properties and Its Effect on Models of Induced Arthritis in Mice and Cystitis in Rats
Authors: Kirikovich Svetlana S. , Levites Evgeniy V. , Proskurina Anastasia S. , Ritter Genrikh S. , Dolgova Evgeniya V. , Ruzanova Vera S. , Oshihmina Sofya G. , Snegireva Julia S. , Gamaley Svetlana G. , Sysoeva Galina M. , Danilenko Elena D. , Taranov Oleg S. , Ostanin Alexandr A. , Chernykh Elena R. , Kolchanov Nikolay A. , Bogachev Sergey S.
Published: September 2024

Vitamin D3 transporter (DBP) is a multifunctional protein. Site-specific deglycosylation results in its conversion to group-specific component protein-derived macrophage activating factor (GcMAF), which is capable of activating macrophages. It has been shown that depending on precursor conversion conditions, the resulting GcMAF activates mouse peritoneal macrophages towards synthesis of either pro- (IL-1β, TNF-α—M1 phenotype) or anti-inflammatory (TGF-β, IL-10—M2 phenotype) cytokines. The condition for the transition of the direction of the inflammatory response of macrophages when exposed to GcMAF is the initial glycosylated state of the population of DBP molecules and the associated effective deglycosylation of DBP by β-galactosidase. In vivo experiments with GcMAF exhibiting anti-inflammatory properties on models of induced arthritis in mice and cystitis in rats indicate a significant anti-inflammatory effect of the macrophage activator. The feasibility of unidirectional induction of anti-inflammatory properties of macrophages allows creation of combined therapeutic platforms where M2 macrophages are among the key therapeutic components.
Integrated MicroRNA-mRNA Analyses of the Osteogenic Differentiation of Human Dental Pulp Stem Cells by a Helioxanthin Derivative
Authors: Fujii Yasuyuki , Minami Sakura , Hatori Ayano , Kawase-Koga Yoko , Ogasawara Toru , Chikazu Daichi
Published: September 2024

Dental pulp stem cells (DPSCs) demonstrate high proliferative and multilineage differentiation potential. As previously reported, the helioxanthin derivative 4-(4-methoxyphenyl)pyrido[40,30:4,5]thieno[2,3-b]pyridine-2-carboxamide (TH) has been demonstrated to induce the osteogenic differentiation of DPSCs. However, the mechanism of osteogenesis induced by TH in DPSCs remains unknown. The objective of this study was to identify functional extracellular vesicle (EV) microRNAs (miRNAs), and the principal genes involved in the TH-induced osteogenesis of DPSCs. DPSCs were derived from dental pulp extracted from the third molars of three healthy subjects, and were cultured with or without TH. miRNAs were extracted from DPSC-derived EVs. The gene expression patterns of mRNA and miRNA were compared using RNA-Seq and miRNA-Seq. To investigate miRNA/mRNA interacting networks, functional analyses were performed by Ingenuity Pathway Analysis. Alkaline phosphatase (ALP) staining demonstrated that treatment with TH resulted in enhanced ALP activity in DPSCs after 7 days. The expression levels of ALP and type 1 collagen alpha 1 were significantly higher in TH-induced DPSCs on day 7. RNA-Seq and miRNA-Seq analyses identified 869 differentially expressed genes (DEGs) and 18 miRNA-DEGs. Gene Ontology analysis of the mRNA-Seq results showed that TH induced several biological activities associated with signal transduction, cell adhesion, and cell differentiation. Integrated miRNA-mRNA analyses showed that these miRNAs contain the targeting information of 277 mRNAs of the DEGs. Among them, 17 target genes known to be involved in the differentiation of osteoblasts, and 24 target genes known to be involved in the differentiation of bone cells were identified. Quantitative real-time PCR showed that WNT5a expression in DPSCs was upregulated by 48 h of TH treatment. Upstream regulator analysis indicated that WNT3a, FOS, and RAC1 may be responsible for gene expression changes in DPSCs after TH treatment. EV miRNA regulatory networks might play crucial roles in TH-induced osteogenic differentiation of DPSCs. Our results presented herein offer valuable insights that will facilitate further research into the mechanism of osteogenesis of DPSCs, which is expected to lead to the clinical application of TH-induced DPSCs for bone regeneration. Furthermore, EVs derived from TH-induced DPSCs might be useful as therapeutic tools for bone defects.
Peptide–Oligonucleotide Conjugation: Chemistry and Therapeutic Applications
Authors: Malinowska Anna L. , Huynh Harley L. , Bose Sritama
Published: September 2024

Oligonucleotides have been identified as powerful therapeutics for treating genetic disorders and diseases related to epigenetic factors such as metabolic and immunological dysfunctions. However, they face certain obstacles in terms of limited delivery to tissues and poor cellular uptake due to their large size and often highly charged nature. Peptide–oligonucleotide conjugation is an extensively utilized approach for addressing the challenges associated with oligonucleotide-based therapeutics by improving their delivery, cellular uptake and bioavailability, consequently enhancing their overall therapeutic efficiency. In this review, we present an overview of the conjugation of oligonucleotides to peptides, covering the different strategies associated with the synthesis of peptide–oligonucleotide conjugates (POC), the commonly used peptides employed to generate POCs, with the aim to develop oligonucleotides with favourable pharmacokinetic (PK) or pharmacodynamic (PD) properties for therapeutic applications. The advantages and drawbacks of the synthetic methods and applications of POCs are also described.
Statistics and Meteorology of Cutoff Lows over South Africa 1970–2023
Authors: Jury Mark R.
Published: September 2024

The meteorology of cutoff lows over South Africa is characterized by statistical analysis of daily field data in the period 1970–2023. An index is formulated by subtracting 500 hPa geopotential height in the mid-latitudes from the subtropics. Cutoff lows (COL) are identified by positive values, mostly in autumn and spring. Statistics indicate that climate forcing is seasonal: La Nina/El Nino favors COL in March–May/September–November. Hemispheric regressions reveal anomalous highs across the southern mid-latitudes when COL are frequent over South Africa. A 14-case composite was formed from the most intense daily COL events in autumn and spring. The composite shows a NW- tilted Rossby wave and jet stream loop around the COL. Maritime easterlies induce a warm east—cool west SST pattern, but composite moist inflows are shallow, so stormy weather hugs the coastal plains. Overturning circulations meet in an upper-level “saddle” over South Africa. 500 hPa sinking motions to the Southwest are of similar strength to rising motions to the Northeast. A COL case study exhibited hourly rain rates >10 mm at Port Alfred 18–20 October 2012 fed by tropical inflow. New insights emerged from this study via composite height sections over South Africa.
Balance, Gait, Functionality and Fall Occurrence in Adults and Older Adults with Type 2 Diabetes Mellitus and Associated Peripheral Neuropathy
Authors: Tavares Natália Maria Bezerra , Silva Jonathânya Marques , Silva Mayra Darlene Morato da , Silva Letícia Danielly Tenório , Souza Jackson Nascimento de , Ithamar Lucas , Raposo Maria Cristina Falcão , Melo Renato S.
Published: September 2024

Background: Body balance is regulated by sensory information from the vestibular, visual and somatosensory systems, and changes in one or more of these sensory systems can trigger balance disorders. Individuals with type 2 Diabetes Mellitus (DM2) often present peripheral neuropathy, a condition that alters foot sensory information and can negatively influence balance and gait performance of these subjects. Objective: To evaluate and compare balance, gait, functionality and the occurrence of falls between individuals with and without a clinical diagnosis of DM2 with associated peripheral neuropathy. Methods: Cross-sectional study, which evaluated seventy individuals, thirty-five with and thirty-five without a clinical diagnosis of DM2, of both sexes and age range between 50 and 85 years, who were recruited from Basic Health Units of Serra Talhada, Pernambuco state, Brazil. The volunteers’ balance was analyzed using the Berg Balance Scale, gait-related functional tasks were measured using the Dynamic Gait Index, functional mobility was assessed using the Timed Up and Go test and functionality was assessed using the Katz Index. The occurrence of falls was recorded by the volunteers’ self-report. Results: Individuals with DM2 demonstrated the worst performance in balance (p = 0.000) and in gait-related functional tasks (p = 0.000), slower functional mobility (p = 0.000) and worse functionality (p = 0.016) compared to the group without DM2, demonstrating significant differences for all analyzed outcomes. A greater occurrence of falls was observed in individuals with DM2, compared to those without the disease (p = 0.019). Conclusion: Individuals with DM2 demonstrated worse performance on balance, gait-related functional tasks, slower functional mobility and worse functionality compared to those without the disease. Individuals with DM2 had the highest occurrence of falls in this study.
Two-Dimensional Materials-Based Thin Films and Coatings
Authors: Tene Talia , Tubon-Usca Gabriela , Haro-Barroso Camilo , Velasco Castelo Geoconda Marisela , Vacacela Gomez Cristian
Published: September 2024

Here, we highlight the research presented in this Special Issue, focusing on the innovative use of graphene and other two-dimensional (2D) materials to develop advanced coating technologies. The contributions herein address critical challenges such as the scalable fabrication and stable dispersion of such materials and their compatibility with conventional coating systems, offering solutions that enhance their mechanical strength, chemical stability, and multifunctionality. The featured studies demonstrate the diverse applications of these materials, from protective anticorrosive barriers to high-performance optoelectronic devices and environmental remediation. Moving forward, future research is encouraged to explore novel 2D materials, hybrid coating strategies, and advanced computational modeling to overcome existing limitations and unlock new possibilities.
Aluminide Coatings by Means of Slurry Application: A Low Cost, Versatile and Simple Technology
Authors: Agüero Alina , Audigié Pauline , Gutiérrez Marcos , Lorente Cristina , Mora Julio , Rodríguez Sergio
Published: September 2024

The present study focused on demonstrating the versatility of the slurry deposition technique to produce aluminide coatings to protect components from high-temperature corrosion in a broad temperature range, from 400 to 1400 °C. This is a simpler and low-cost coating technology used as an alternative to CVD and pack cementation, which also allows the coating of complex geometries and offers improved and simple repairability for a lot of industrial applications, along with avoiding the use of non-hazardous components. Slurry aluminide coatings from a proprietary water-based-Cr6+ free slurry were produced onto four different substrates: A516 carbon steel, 310H AC austenitic steel, Ti6246 Ti-based alloy and TZM, a Mo-based alloy. The resulting coatings were thoroughly characterised by FESEM and XRD, mainly so that the identification of microstructures and appropriate phases was reported for each coating. The importance of surface preparation and heat treatment as key parameters for the coating final microstructures was also evidenced, and how those parameters can be optimised to obtain stable intermetallic phases rich in Al to sustain the formation of a protective Al2O3 oxide scale. These coating systems have applications in diverse industrial environments in which high-temperature corrosion limits the lifetime of the components.
Investigation of the Corrosion–Wear Interaction Behavior of 8Cr4Mo4V Bearing Steel at Various Corrosion Intervals
Authors: Zhao Chao , Ying Lixia , Nie Chongyang , Zhu Tianlin , Tang Rongxiang , Liu Ruxin
Published: September 2024

The corrosion–wear coupling damage failure of 8Cr4Mo4V bearing steel under marine atmospheric conditions significantly limits aeroengine bearing applications. The present work aims to investigate the evolution of the corrosion–wear properties of 8Cr4Mo4V bearing steel at varied corrosion intervals and estimate the corrosion–wear interaction (CWI) effect. Neutral salt spray tests combined with tribological experiments were employed to explore the effect of corrosion on wear and the influence of wear on corrosion, and a quantitative characterization method of corrosion–wear interactions was proposed by establishing the component relationships of material losses in the corrosion–wear process. The results indicate that the corrosion rates initially increase and then decrease, ultimately resulting in a pattern characterized by predominant total corrosion and nested localized corrosion. The corroded surfaces tremendously influence the friction coefficient curves at the third stage, and a synergistic acceleration effect exists in the CWI behavior of 8Cr4Mo4V bearing steel under the action of corrosion and wear. A sample corroded for 6 h displayed the significant facilitative effect of corrosion on wear, exhibiting the highest CWI ratio and a greater total mass loss primarily attributed to corrosion. This study offers a significant reference for the quantitative assessment of the tribo-corrosion properties of bearings in a marine atmospheric environment.
Study on the Performance of Modified Qingchuan Rock/Rubber Asphalt
Authors: Li Wuan , Chen Yukun , Guo Tengteng , Li Zhenxia , Jiang Xiaoxiao
Published: September 2024

This paper developed a new environmentally friendly composite modified asphalt material and studied the composite modification of Qingchuan rock asphalt (QRA) and waste tire rubber powder (RP) was studied in this paper. QRA/RP composite modified asphalt was prepared by adding these two materials as modifiers into matrix asphalt and compared with matrix asphalt and QRA modified asphalt. The basic properties of asphalt before and after aging were evaluated by the rotating thin film oven test. The high-temperature performance and permanent deformation resistance at different temperatures and frequencies were analyzed by the dynamic shear rheological test. The bending creep stiffness test was used to evaluate the low-temperature performance. In addition, the microstructure and modification mechanism of composite-modified asphalt were analyzed by scanning electron microscopy and infrared spectroscopy. The results show that QRA-modified asphalt is superior to matrix asphalt in terms of mass loss, viscosity ratio, and residual penetration, while QRA/RP composite-modified asphalt is further improved on this basis, QRA/RP composite modified asphalt can effectively improve the high and low temperature performance of asphalt.. Although the addition of RP is mainly based on physical modification, it also causes weak chemical reactions and enhances the adhesion of asphalt. The interaction between Qingchuan Rock asphalt and rubber powder significantly improves the overall stability of asphalt structure.
Modelling and Experimental Validation of the Flame Temperature Profile in Atmospheric Plasma Coating Processes on the Substrate
Authors: Martínez-García Jose , Martínez-García Venancio , Killinger Andreas
Published: September 2024

This work presents a characterisation model for the temperature distribution at different substrate depths during the atmospheric plasma spray (APS) coating process. The torch heat flow in this model is simulated as forced convection defined by a surface, a temperature profile, and a convection coefficient. The simulation model considers three plasma temperature profiles of the Al2O3 coating on a 5 mm thickness flat aluminium substrate. The simple and low-cost experimental procedure, based on a thermocouple, measures the plasma plume temperature distribution of the APS coating system, and their results are used to obtain the parameter values of each of the three proposed plasma temperature profiles. The experimental method for in situ non-contact temperature measurements inside the substrate is based on an infrared pyrometry technique and validates the simulation results. The Gaussian temperature profile shows excellent accuracy with the measured temperatures. The Gaussian approach could be a powerful tool for predicting residual stress through a coupled one-way thermal-mechanical analysis of the APS process.
A New Investigation on Dynamics of the Fractional Lengyel-Epstein Model: Finite Time Stability and Finite Time Synchronization
Authors: Almimi Hani Mahmoud , Abu Hammad Ma’mon , Farraj Ghadeer , Bendib Issam , Ouannas Adel
Published: September 2024

In this paper, we present an investigation into the stability of equilibrium points and synchronization within a finite time frame for fractional-order Lengyel–Epstein reaction-diffusion systems. Initially, we utilize Lyapunov theory and multiple criteria to examine the finite-time stability of equilibrium points. Following this analysis, we design efficient, interdependent linear controllers. By applying a Lyapunov function, we define new adequate conditions to ensure finite-time synchronization within a specified time interval. Finally, we provide two illustrative examples to demonstrate the effectiveness and practicality of our proposed method and validate the theoretical outcomes.
Why Are Other Teachers More Inclusive in Online Learning Than Us? Exploring Challenges Faced by Teachers of Blind and Visually Impaired Students: A Literature Review
Authors: Ghoneim Rana , Aljedaani Wajdi , Bryce Renee , Javed Yasir , Khan Zafar Iqbal
Published: September 2024

Distance learning has grown rapidly in recent years. E-learning can aid teachers of students with disabilities, particularly visually impaired students (VISs), by offering versatility, accessibility, enhanced communication, adaptability, and a wide range of multimedia and non-verbal teaching methods. However, the shift from traditional face-to-face instruction to online platforms, especially during the pandemic, introduced unique challenges for VISs, with respect to including instructional methodologies, accessibility, and the integration of suitable technology. Recent research has shown that the resources and facilities of educational institutions pose challenges for teachers of visually impaired students (TVISs). This study conducts a literature review of research studies from the years 2000 to 2024 to identify significant issues encountered by TVISs with online learning to show the effects of distance learning before, during, and after the pandemic. This systematic literature review examines 25 publications. The evaluation reveals technological problems affecting the educational experience of visually impaired educators through a methodical categorization and analysis of these papers. The results emphasize important problems and suggest solutions, providing valuable knowledge for experts in education and legislation. The study recommends technology solutions to support instructors in providing inclusive online learning environments for VISs.
Influence Mechanisms of Cold Rolling Reduction Rate on Microstructure, Texture and Magnetic Properties of Non-Oriented Silicon Steel
Authors: Guo Feihu , Niu Yuhao , Fu Bing , Qiao Jialong , Qiu Shengtao
Published: September 2024

The effects of cold rolling reduction on the microstructure, recrystallization behavior, and magnetic properties of 3.0%Si-0.8%Al-0.3%Mn steel were studied by X-ray diffraction (XRD) and electron backscatter diffraction (EBSD). With the reduction rates of 78%, 85% and 87% in the cold rolled sheet, the width of the deformation band becomes narrower, the number of intragranular shear bands decreases, and the proportion of grain boundaries increases. The intensity of the α and γ fibers texture in the cold rolled sheet is enhanced, and the annealed sheet is dominated by the γ fibers texture and the content increases from 26.0% to 34.5%. During the recrystallization process, the Goss and γ-grains nucleate first. The λ-grains nucleate mainly at the grain boundaries of the deformed α-grains, and the α-grains ultimately recrystallize. With the increase in the cold rolling reduction rate, the γ-grains develop into the main texture due to a large amount of nucleation at the deformation band and grain boundary. The λ-grains with a high mobility do not have a numerical advantage, and the increase in the texture content is very small. The content of the unfavorable γ fiber texture in the annealed sheet increases, the magnetic induction intensity B50 decreases, Pe and Pt decrease significantly, and the critical grain size with the lowest iron loss decreases from 136.2 to 109.4 μm.
Enhancing Shear Bond Strength in Lithium Silicate Glass Ceramics: Surface Treatment Optimization for Reseating Protocols
Authors: Torbiak Allison , Bebsh Muna , Haimeur Asmaa , Fernandes Ana Carla , Fiuza Cristina , França Rodrigo
Published: September 2024

The rapid evolution of lithium silicate-based glass ceramics in the field of dental ceramics has led to the availability of different compositions in the market. This in vitro study was conducted to assess an effective protocol for recementing de-bonded lithium silicate-based glass ceramics by evaluating the shear bond strength of three reseating methods. The study included IPS e.max® CAD, Vita Suprinity®, Celtra Duo®, and n!ce as lithium-based glass ceramics. The samples underwent a series of preparation steps, including embedding in acrylic resin, hand polishing, etching with 5% hydrofluoric acid, and application of universal primer and adhesive as per manufacturer instructions. Subsequently, adhesive resin cement was applied to the ceramic tablets, and shear bond strength was assessed using a standardized method. The findings revealed that no single method demonstrated significantly superior results compared to the others. However, it was observed that etching with 5% hydrofluoric acid for 20 s yielded favorable outcomes in terms of time efficiency and standardized results. Additionally, it was noted that although sandblasting increased surface area, it did not enhance bond strength due to unfavorable surface disturbance. In conclusion, the study suggests that etching with 5% hydrofluoric acid for 20 s is a favorable protocol for reseating de-bonded lithium disilicate-based glass ceramics, offering both time efficiency and consistent results for clinicians.