bluuebunny/crossref_metadata_2025 huggingface dataset:
Matrix-assisted laser desorption ionization time-of-flight mass spectrometric analysis of glycosphingolipids including gangliosides.
Authors: Taketomi T , Hara A , Uemura K , Sugiyama E
Published: January 2020

Long chain base compositions of gangliosides containing mainly stearic acid could be determined without any chemical modification by matrix-assisted laser desorption ionization time-of-flight mass spectrometry with delayed ion extraction (DE MALDI-TOF MS). The analytical results for the long chain base compositions of various samples of GM1 from the brain tissues of patients with different diseases at different ages confirmed that the proportion of d20:1 (icosasphingosine) and d20 (icosa-sphinganine) of the total sphingosine bases increased quickly until adolescent or adult age and then remained constant slightly exceeding 50%; this value was evidently higher than the proportion of d20:1 and d20 of GM1 in various adult mammalian brains. A long chain base composition of GM1 from the brain tissue of a patient with infantile type of GM1-gangliosidosis at 4y2m was abnormal and so was in two sibling patients with Spielmeyer-Vogt type of juvenile amaurotic idiocy at 19y and 21y in spite of that in the latter there was no accumulation of GM1 in the brain tissue. On the other hand, a patient with adult type of GM1 gangliosidosis at 66y showed a local accumulation of GM1 in the putamen and caudate nucleus, but its long chain base composition was found to be normal. It was of interest that the white matter of Eker rat with hereditary renal carcinoma contained a large amount of plasmalocerebroside as compared with the amount of cerebroside and sphingomyelin. The individual molecular species of plasmalocerebroside were identified by DE MALDI-TOF MS.
Occurrence of lipopolysaccharide alterations among Tn5 mutants of Rhizobium leguminosarum bv. trifolii strain 24.1 with altered colony morphology.
Authors: Szyprowska S , Choma A , Glowacka M , Gronek E , Kitlińska A , Rola M
Published: January 2020

Transposon mutants of Rhizobium leguminosarum bv. trifolii 24.1 showing less glossy or smaller colonies were screened for properties usually associated with lipopolysaccharide (LPS) defects in R. leguminosarum, i.e. motility, growth rate, tendency to agglutination in liquid media and symbiotic efficiency. Neither any of the above mutants nor the earlier isolated 24.12 strain, defective in LPS, showed all these properties changed simultaneously. According to PAGE/sodium deoxycholate analysis the mutant 24.12 was the only one producing defective lipopolysaccharide. GC-MS analysis revealed in this mutant qualitative changes in composition of its LPS in comparison with LPS isolated from the parent strain. Other Tn5 mutants produced LPSs similar in composition, however the proportion between LPS I and LPS II differed from that in the parent strain.
Collagenous constituents of amniotic fluid.
Authors: Gogiel T , Bielecki D A , Bańkowski E
Published: January 2020

The amniotic fluid (AF) was fractionated by dialysis, gel filtration and SDS/PAGE, and submitted to the assay of collagenous constituents. The collagenous character of peptides and proteins of amniotic fluid was confirmed by hydroxyproline (Hyp) assay and treatment with bacterial collagenase followed by electrophoresis and gel filtration of the digestion products. It was found that AF contains collagen degradation products but the classical method of Hyp determination described by Woessner (Arch. Biochem. Biophys., 1961, 93, 440-447) gives overestimated values due to the interference with other AF components. Fractionation of AF on Sephadex G-100 column allowed to remove the interfering material and to estimate the actual Hyp content which equals to approx. 6.2 microg/ml. About 70% of Hyp was found in low molecular dialyzable products and the rest (about 30%) appears to be a constituent of nondialyzable collagenous polypeptides of the molecular mass of about 7.9-26.3 kDa. It is suggested that such collagenous polypeptides may be the products of proteolytic conversion of collagen precursor (procollagen) into the monomeric form of this protein. No high molecular forms of collagen, corresponding to alpha-subunits, were found.
Molecular characterization and symbiotic importance of prsD gene of Rhizobium leguminosarum bv. trifolii TA1.
Authors: Mazur A , Wielbo J , Król J , Kopcińska J , Lotocka B , Golinowski W , Skorupska A
Published: January 2020

The prsD, prsE and orf3 genes of Rhizobium leguminosarum bv. trifolii strain TA1 encode the proteins which are significantly related to the family of bacterial ABC transporters type I secretion systems. The prsD:Km(r) mutant of strain TA1 induced non-nitrogen-fixing nodules on Trifolium pratense. Microscopic analysis of the nodules induced by prsD mutant did not reveal major abberations in the bacteroid appearance. The exopolysaccharide of prsD mutant was produced in increased amount and its level of polymerization was changed. SDS/PAGE of the proteins from the culture supernatants showed a lack of the 47-kDa protein in the culture of prsD mutant. Thus, PrsD may play a role in the export of this protein.
Decomposition of lipid hydroperoxides enhances the uptake of low density lipoprotein by macrophages.
Authors: Babiy A V , Gebicki J M
Published: January 2020

This study examined the roles of low-density lipoprotein (LDL) lipid oxidation and peroxide breakdown in its conversion to a form rapidly taken up by mouse peritoneal macrophages. Oxidation of the LDL without decomposition of the hydroperoxide groups was performed by exposure to gamma radiation in air-saturated solutions. Virtually complete decomposition of the hydroperoxides was achieved by treatment of the irradiated LDL with Cu2+ under strictly anaerobic conditions. No uncontrolled LDL uptake by macrophages occurred when the lipoprotein contained less than 150 hydroperoxide groups per particle. More extensively oxidized LDL was taken up and degraded by mouse macrophages significantly faster than the native lipoprotein. The uptake was greatly enhanced by treatment of the oxidized LDL with Cu2+. A significant proportion of the LDL containing intact or copper-decomposed LDL hydroperoxide groups accumulated within the macrophages without further degradation. Treatment of the radiation-oxidized LDL with Cu2+ was accompanied by aggregation of the particles. Competition studies showed that the oxidized LDL was taken up by macrophages via both the LDL and the scavenger receptors, whereas the copper-treated lipoprotein entered the cells only by the scavenger pathway. Phagocytosis also played an important role in the metabolism of all forms of the extensively modified LDL. Our results suggest that minimally-oxidized LDL is not recognized by the macrophage scavenger receptors unless the lipid hydroperoxide groups are decomposed to products able to derivatize the apo B protein.
Differential regulation of signaling pathways for insulin and insulin-like growth factor I.
Authors: Lopaczynski W
Published: January 2020

The insulin receptor (IR) and the insulin-like growth factor receptor I (IGF-IR) have different functions in cell growth, apoptosis, differentation, and transformation. Although some of these differences may be explained by the relative level of receptor expression and receptor structure (alpha and beta subunits), they may also be attributed to differences in intracellular signals generated by insulin and IGF-I. The presence of hybrid receptors (IR alphabeta subunits and IGF-IR alphabeta subunits) making up the heterotetramers has added a new dimension to our understanding of the functional roles of these receptors. However, to date the results of efforts to understand the differences between these two closely related receptors have indicated mostly similarities. For example, both receptors utilize IRS-1/IRS-2 and Shc as immediate downstream adaptors, leading to activation of the Ras, Raf, ERK kinases and PI-3 kinase pathways. We have used the yeast two hybrid system to identify proteins which bind to the activated IGF-IR but not to the IR. The cytoplasmic domain of the IGF-IR was used to screen a human fetal brain library and two isoforms of the 14-3-3 family were identified. 14-3-3 proteins are a highly conserved family of proteins which have recently been shown to interact with other components of the mitogenic and apoptotic signaling pathways, including Raf, BAD, Bcr/Bcr-Abl, middle-T antigen, Ksr, PKC, PI-3 kinase, ASK1 kinase, and cdc25C phosphatase. We also identified human Grb10, an adaptor protein with SH2 domain associated with the IGF-IR beta subunit. Smith's laboratory showed that Grb10 preferentially binds to the IR in intact cells. Using the interaction trap screen (active cytoplasmic domain of the IGF-IR) 55PIK and SOCS-2 proteins were also identified. However, 55PIK and SOCS-2 also interact with the IR in the yeast two hybrid system. These studies raise the possibility that 14-3-3 and Grb10 may play a role in insulin and IGF-I signal transduction and may underlie the observed differences.
The role of cholesterol and sphigomyelin in tyrosine phosphorylation of proteins and capping of Fcgamma receptor II.
Authors: Drzewiecka A , Kwiatkowska K , Sobota A
Published: January 2020

Cross-linking of cell surface receptors by multivalent ligands, e.g. by antibodies, evokes their clustering -- patching. Subsequently, these clusters can be translocated by the acto-myosin machinery toward one pole of the cell and assembly cap. Patching of FcgammaRII in U937 cells correlates with tyrosine phosphorylation of several proteins while cap assembly correlates with their dephosphorylation. To study the mechanism of activation of tyrosine kinases during FcgammaRII activation we disturbed the organization of the putative plasma membrane microdomains by depletion of membrane cholesterol and sphingomyelin. Cholesterol was removed with the use of beta-cyclodextrin while sphingomyelin was decomposed by exogenous sphingomyelinase. Cyclodextrin at 5-10 mM removed about 70% of cholesterol from the cells and abolished the assembly of FcgammaRII caps thereby arresting the receptors at the patching stage. Similarly, 70 mU/ml sphingomyelinase inhibited cap formation by 60%. Cholesterol and sphingomyelin depletion also suppressed the tyrosine phosphorylation of proteins which accompanied cross-linking of FcgammaRII. The observations indicate that cholesterol and sphingomyelin can control the interactions of tyrosine kinases with clustered FcgammaRII.
Insulin-like growth factor I activates insulin receptor substrate 1 and Ras in human osteosarcoma cells.
Authors: Lopaczynski W , Terry C
Published: January 2020

Insulin-like growth factor I (IGF-I) stimulates multiplication of the human osteosarcoma cell line, MG-63, by acting through IGF-I receptor. We have characterized IGF-I stimulated phosphorylation of IRS-1, activation of Ras cycle and phosphorylation of c-Jun in this cell line. Serum starved MG-63 cells were (1) IGF-I stimulated and lysates were immunoprecipitated with polyclonal IRS-1 antibody or (2) metabolically labeled with [32P]orthophosphoric acid and then cells were treated with IGF-I. Cell lysates were immunoprecipitated with p21Ras antibody (Y13-259) and bound nucleotides were analysed by thin-layer chromatography. We demonstrated tyrosine phosphorylation of IRS-1/2 immunoprecipitated from MG-63 cells stimulated with IGF-I. We also showed an increased level of GTP in p21Ras immunoprecipitates from IGF-I treated cells. Nuclear extracts prepared from 32P-labeled cells before and after addition of IGF-I were immunoprecipitated with c-Jun antibody. After electrophoresis and autoradiography, phosphorylation of the c-Jun band was seen to be IGF-I independent. Phosphoamino acid analysis of the c-Jun band showed that phosphoserine was the major species.
Sphingosine, sphingosylphosphorylcholine and sphingosine 1-phosphate modulate phosphatidylserine homeostasis in glioma C6 cells.
Authors: Wójcik M , Barańska J
Published: January 2020

The effect of sphingosine, sphingosylphosphorylcholine and sphingosine 1-phosphate on L-[U-14C]serine incorporation into phosphatidylserine and phosphatidylserine-derived phosphatidylethanolamine was investigated in intact glioma C6 cells. Sphingosine, sphingosylphosphorylcholine and sphingosine 1-phosphate are potent signalling molecules which, due to their physicochemical features, may function as amphiphilic compounds. It has been found that sphingosine and sphingosylphosphorylcholine (amphiphilic cations) significantly increase [14C]phosphatidylserine synthesis and decrease the amount of 14C-labeled phosphatidylethanolamine. Sphingosine 1-phosphate (an amphiphilic anion) was without effect on phosphatidylserine synthesis but, similarly as sphingosine and sphingosylphosphorylcholine, reduced the conversion of phosphatidylserine to phosphatidylethanolamine. These results strongly suggest that sphingosine, sphingosylphosphorylcholine and sphingosine 1-phosphate can modulate cellular phospholipid homeostasis by stimulation of phosphatidylserine synthesis and an interference with phosphatidylserine decarboxylase.
Heterologous expression of genes in filamentous fungi.
Authors: Kruszewska J S
Published: January 2020

Isolation of some biologically important proteins from natural sources was found to be too expensive or scarcely possible (human proteins). The problem could be solved by expression of heterologous genes. Many biologically active proteins have been successfully expressed in filamentous fungi, some of them, however, at a low level. Thus, improvement of this technique appears to be a very important task. The process comprises several steps. Some of them, such as efficient transformation, vector construction, processing of signal sequences, post-translational modifications and secretion of the expressed proteins, have been intensively investigated. This review presents obstacles and problems encountered in expression of heterologous genes and discusses strategies of development in this area.
Expression of ras-family genes in the cell cycle and during differentiation of the lower eukaryote Physarum polycephalum.
Authors: Fronk J
Published: January 2020

Messenger RNA levels of three ras-family genes (Ppras1, Ppras2, and Pprap1) were measured in different life forms and throughout the cell cycle of the slime mold Physarum polycephalum. All three genes are expressed at constant rates in the uninucleate amoebae and flagellates, regardless of the culture conditions (solid or liquid medium, particulate or dissolved nutrients). In the multinucleate stages (micro- and macroplasmodia) Ppras1 and Pprap1 mRNAs are somewhat less abundant, while Ppras2 is not expressed at all. The early stages of the amoeba-plasmodium transition proceed without any drop in Ppras2 expression. During the synchronous cell cycle in macroplasmodia Ppras1 and Pprap1 are expressed at a constant level.
Effects of the Agri-environmental Program Implemented in Sultan Reeds Area of Kayseri, Turkey
Authors: Boz Ismet
Published: January 2020

This study was initiated to evaluate the effects of agri-environment program implemented in the Sultan reeds area of Kayseri province, Turkey. The specific objectives of the study were to compare the farmers who enrolled in the program with those who didn’t enroll regarding their application of different sustainable agricultural practices, and to determine factors affecting their enrolment in the program. The main comparative indicators were selected from different sustainable agricultural practices either promoted by the agri-environmental program or not promoted but considered very useful for the locality. Two stratified samples of farmers (enrolled and not enrolled) were selected based on their farm size. Chi-square tests of independence were used to compare farmers on the selected sustainable agricultural practices. Logistic regression model was used to determine factors affecting the enrolment of the agri-environment program. The findings of the chi-square test showed that enrolled farmers use grow more forage legumes, are more conscious about pesticides use and chemical applications, and they use more pressurized irrigation systems. Findings of the logistic regression model sowed that using rental land negatively, but contacts with extension personnel, and using long term loans for farming investments positively influenced the enrolment of the agri-environment program. Governmental effort must concentrate on these issues when promoting agri-environmental programs in the region.
Assessing the Influence of Responsive Disorder on the Academic Performance of Married Post-graduate Students in North eastern Universities in Nigeria
Authors: Christian Igomu Amuche , Naomi Nuhu Adamu
Published: January 2020

The study assessed the influence of Responsive disorder on the academic performance of married post-graduate students in North Eastern Universities in Nigeria. Two research questions and two null hypotheses were formulated in harmony with the objectives of the study. The design of this study is survey. The population of the study consist of 22,772 married post-graduate students from 4 Universities in North-east, Nigeria. The sample size for the study was 422 determined using the Proportionate sampling strategy employed at different levels in selection of postgraduate students from the four Universities and along gender lines. The researcher developed two instruments for data collection for the study titled: Students’ Responsive disorder Inventory and Research and Statistics Achievement Test. The reliabilities of the instruments yielded 0.84 and 0.87 reliability coefficients of internal consistency for SRDI and RSAT respectively. Descriptive statistics were used to summarize data related to the research questions. Linear regression statistic was used to test the formulated null hypotheses. It was found that postgraduate students face Responsive disorder even with an above average level academic performance. However, Responsive disorder significantly influenced academic achievement of postgraduate students who are married in Universities in North-east Nigeria. Also, Responsive disorder significantly predicts academic achievement of postgraduate students irrespective of gender. The study concludes that there is relatively high level agreement among postgraduate students in the North-east states with respect to the Responsive disorder faced by them. The finding debunks traditional beliefs that married people are more likely to perform poorly or even drop-out. This study found support for the position that married people may beat the single and even complete their studies before the singles. The study recommends among others that; coping strategies be evolved to help resolve the debacle; Scholarships should be awarded to students to reduce the financial burden, and, efforts should be invested in promoting emotional health among post-graduate students in order to mellow the negative effect on their academic performance.
Examination of Self-efficacy Beliefs of on performance (An empirical study on National level Players of team sports in Sri Lanka)
Authors: HPN Perera , M Jusoh , SMF Azam , SRSN Sudasinghe
Published: January 2020

The main goal of this study was identify the impact of Self-Efficacy on the performance of team sports players in Sri Lanka. Mainly it was focused to measure self-efficacy belief of team players and the experimental variable of the study was perceived performance. The study utilized a likert scale questionnaire which had been adopted from literature to obtain data for the study. The research model was tested using 308 subjects comprised of national level team players. Data were analyzed using SPSS and structural equation modeling with AMOS. Self-efficacy has proven to have a noticeable impact on subjective performance of the players. The recommendations included the strategies which can be utilized to enhance the self-efficacy belief of the players.
The Influence of Television Advertising in the Promotion of Yoruba Attires in Ondo State: a Study of NTA Akure
Authors: Bejide, Oluwatoyin Abiola  , Olaleye, Franklyn Akinola  , Adekoya, Adeola Ojo
Published: January 2020

This project is based on the influence of television advertising in the promotion of yoruba attires in Ondo State, “a study of NTA Akure”. The research work review related literature and was anchored on persuasive media and cultural conservative theories. It also employed survey design and questionnaire was administered to respondents of the selected three local government areas in Ondo State, but out of the 400 copies of questionnaire administered, 370 was duely attested and recovered for data analysis. The findings revealed that, NTA Akure advertising positively influenced the promotion of Yoruba attires in Ondo State. The study also revealed that, the level of the level of cognizance giving to the promotion of traditional attires especially Aso-Oke by NTA Akure is low. The study concludes that, television advertising has positively influenced the promotion of traditional attires in Yoruba society through assessment of NTA Akure viewers. This research work recommends that, NTA Akure should give much more cognizance to the promotion of the traditional attires through advertisement programme. It also recommends that the Ministry of Art and Culture in Ondo State should see NTA Akure as a medium through which cultural heritage especially traditional attires can be promoted.
PROJETO DE CONTROLADORES PID ROBUSTOS VIA OTIMIZAÇÃO NUMÉRICA COM APLICAÇÃO NO CONTROLE DE NÍVEL
Authors: Leonardi Fabrizio , Aguiar Renato Aparecido , Franco Ivan Carlos , Menezes Mauro Renault
Published: January 2020

O estudo de sistemas dinâmicos é um problema frequente nas diferentes áreas da Engenharia.  Quando as especificações de desempenho relacionadas a esses tipos de sistemas são rigorosas, normalmente se utilizam sistemas de controle em malha fechada para garantir o desempenho esperado, mesmo na presença de erros de modelagem, ou seja, robustos às incertezas. O presente trabalho propõe a aplicação da técnica de shooting de otimização como alternativa para o projeto de controladores PID robustos, sendo aplicada a uma planta de controle de nível. Pelos resultados experimentais observados, pode-se concluir que a técnica pode conferir bom desempenho de regime permanente e transitório, avaliados pelo erro estacionário, tempo de acomodação e overshoot mesmo na presença de incerteza no valor da constante de tempo do modelo da planta.
FAST ADAPTIVE GENERALIZED PREDICTIVE CONTROL FOR SYSTEMS WITH VARIABLE PARAMETERS
Authors: ROVEA S. B. , FLESCH RODOLFO
Published: January 2020

This paper proposes a fast predictive control structure with online model update according to process parametric variations. The proposed controller is based on the Generalized Predictive Control (GPC) algorithm, but it integrates the recursive least squares identification method with a variable forgetting factor to estimate at each iteration the parameters of a linear structure model used for multi-step ahead prediction. For a system with constraints on the process variables, the resulting optimization problem of GPC is solved using quadratic programming based on the Alternate Direction Method of Multipliers, which allows the control signal to be obtained with small computational effort. In order to validate the proposed algorithm an experimental case study that considers the speed control of a direct current motor and the proposed controller embedded in a microcontroller STM32F303K8T6 is presented. Experimental results use as baseline the GPC with fixed model parameters and show that the proposed fast adaptive predictive control structure is able to keep almost the same transient response for all the considered operating points of the motor, while GPC presents high oscillations at operating conditions far from the one used to obtain the nominal model. Even though the proposed controller needs to solve two optimization problems at each sampling instant, it can run about 60 times in a second in the microcontroller used in this study
ESTUDO DA VIABILIDADE E DESENVOLVIMENTO DE FILMES ATIVOS A PARTIR DE RESÍDUOS DA INDÚSTRIA VINÍCOLA
Authors: Dias Helen Cristiny , Reis Arlete Barbosa
Published: January 2020

Devido à conscientização frente ao descarte de embalagens e aproveitamento de resíduos industriais, aumentou-se o interesse em desenvolvimento de embalagens ativas com aditivos naturais oriundos de rejeitos. É notória a extensão da produção de uvas no Brasil e o impacto gerado pelo descarte residual do processamento da fruta. Tendo em vista a produção de vinhos e sabendo que a casca da uva, principal componente do resíduo, possui em sua composição agentes com atividade antioxidante, este trabalho visa o desenvolvimento de filmes a base de quitosana com casca de uva para utilização em embalagens ativas. Neste trabalho realizou-se algumas análises para caracterização, envolvendo espessura, solubilidade, permeabilidade de vapor d’agua, análise colorimétrica e MEV. Os resultados demonstram que a adição da casca de uva, influenciou nas características do filme, indicando sua viabilidade.
RATIONAL DRUG DESIGN OF POTENT V600E-BRAF KINASE INHIBITORS THROUGH MOLECULAR DOCKING SIMULATION
Authors: Umar Bello Abdullahi , Uzairu Adamu , Shallangwa Gideion Adamu , Uba Sani
Published: January 2020

B-RAF is a one of the RAF protein kinase group that contribute to the development of different types of cancer. V600E-BRAF protein has lot potential for scientific investigation as the therapeutic target owing to its participation in melanoma cancer and is the molecular target of many anticancer compounds like quinolinylaminopyrimidines (QAP) derivatives. In this research, interactions of QAP derivatives with V600E-BRAF kinase were modeled and predicted using molecular docking simulation approach with the help of Autodock vina version 4.0 of Pyrx software. The molecular docking simulation result of this research shows that QAP6 (−11.7 kcalmol-1) best inhibit V600E-BRAF when compared with other QAP analogous within the dataset and was found to be better than the standard V600E-BRAF inhibitor vemurafenib (-11.3 kcalmol-1). This compound (QAP6) were further used in designing novel and potent V600E-BRAF inhibitors by attaching substituents to the quinoline ring of the compound. Moreover, the two newly designed inhibitors N1 and N2 with a binding energy of −12.7 kcalmol-1 and –12.9 kcalmol-1 respectively were found to be more potent than the parent structure QAP6 (−11.7 kcalmol-1) and the standard V600E-BRAF inhibitor vemurafenib (-11.3 kcalmol-1). Thus; this study provides a valuable approach and new direction to novel drug discovery. There is hope in the future studies to include the synthesis and evaluation of these newly designed inhibitors which can establish them to be the most potent V600E-BRAF inhibitors and efficient ant-melanoma cancer drug.
Long-term care system for dependent people – Austrian experiences
Authors: Luty-Michalak Marta , Syryt Aleksandra
Published: January 2020

Austrian society is an ageing society. Old age does not always mean dependence. However, the risk of disability and dependence increases with age. In addition, older people often experience multi-disease. High-quality long-term care services can help frail and dependent elderly on maintaining greater autonomy and participation in society, regardless of their condition. The aim of the article is to analyze legal, institutional and practical solutions in the field of long-term care system functioning in Austria. It should be emphasized that Austria is striving to develop services based on a social model and an independent life paradigm. Analysis of legal solutions indicates that the long-term care system in Austria is very complex. Institutional solutions are divided between the federal level and nine federal states. On the one hand, this results in decentralization and more effective help for the elderly, but on the other hand, it causes the diffusion and heterogeneity of standards.
THE DEVELOPMENT OF SCIENTIFIC WRITING SKILLS IN PESANTREN: A COMPARATIVE ANALYSIS ON MA’HAD ALY SUKOREJO SITUBONDO AND MA’HAD ALY MANGGISAN WONOSOBO
Authors: Murtadlo Muhamad
Published: January 2020

This paper will examine the scientific writing skills in the context of pesantren. The pesantren has a long scientific history in Indonesia, unfortunately, it has not been accompanied by a strong tradition of scientific writing. The research question formulated is to figure out what causes the tradition of scientific writing in pesantren is not that strong. To find out the answer, this study will use a qualitative approach by raising two pesantren cases that organize ma’had aly education, namely the Ma’had Aly Sukorejo Situbondo and the Ma’had Aly Manggisan Wonosobo.  This  study  found  that  the reason why  the tradition  of  writing in pesantren is not that strong is due to the lack of scientific writing being introduced in pesantren. Eventhough, they have been introduced to the scientific tradition and academic culture in deciding various socio-religious issues. This study recommends the need for a pesantren breakthrough in the developing of scientific writing culture.
EXPERIENCED TEACHERS’ PERCEPTIONS: MATH-FOCUSED STEAM LEARNING
Authors: Rosikhoh Dewi , Mardhiyatirrahmah Liny , Abdussakir Abdussakir , Abtokhi Ahmad , Rofiki Imam
Published: January 2020

Science, technology, engineering, arts, and mathematics (STEAM) have been applied in various countries because they have been able to answer the challenges of the globalization era. The implementation of STEAM at school has demanded that teachers have a role in making a relationship between disciplines in science contained in STEAM. The purpose of this research was to describe the perceptions of experienced teachers about math-focused STEAM learning. The research was a descriptive study on 14 vocational high school mathematics teachers in Malang, Indonesia. They had 20-30 years of teaching experience. Data were collected using essay questionnaires. The result showed that their perceptions of STEAM learning were positive. However, they also said that there were several obstacles to math-focused STEAM learning. The first obstacle was the unavailability of supporting literature. The second obstacle was that there were teachers who have no received training. The third obstacle was only a limited number of mathematics material that can be applied in STEAM learning. Additionally, the teacher stated that mathematics was more suitable as supporting contain in STEAM learning than as the primary focus.
IDENTIFICATION OF SOCIAL SYMPTOMS USING THE DRONE EMPRIT ACADEMIC AS A SUPPORT FOR STATISTICAL LITERACY
Authors: Adji Waluyo Satrio , Bashith Abdul , Nasith Ali , Amin Saiful
Published: January 2020

Social problems in Indonesia in particular are caused by social phenomena transmitted to users of social media, especially Twitter. Big data system provided by Drone Emprit Academic is able to find social phenomena. The ability of critical literacy to read and write supported by statistical data is very important in the 4.0 era. The aim of the research is to find out which Drone Emprit Academic works, analyzes, and displays data on social phenomena whose results can be used to support critical Literacy. This research uses a qualitative approach, literature study method. The analysis includes three stages, namely organize, synthesize, identify. The results of this study that Drone Emprit Academic is a big data system that carries out social network analysis of specific conversations on Twitter in semi-realtime and detail. The form displayed is in the form of a percentage of trends, retweet relationships, mentioning trend graphs, most retweet statuses, conversation trends. The data generated can help read information about social phenomena so that it can support critical literacy which has been partially published in online and offline media.
Tricyclic Antidepressants in Chronic Low Back Pain : A Review
Authors: Ilmanita Dzikrina , Hidayati Hanik Badriyah
Published: January 2020

Chronic low back pain (LBP) is the frequent chronic pain and notable health problem which is the main reason of disabling conditions, individual suffering, sick leave, and costly treatment.  Unfortunately, available therapy especially oral medicines only beneficial in no more than 50% of patients. Tricyclic antidepressants (TCAs) are commonly used for neuropathic pain but the data for neuropathic LBP is limited. The purpose of this review is to evaluate the efficacy and adverse drug reaction of TCAs in chronic LBP. We searched scientific search engines including Scopus and PubMed and collected original articles including Randomized Clinical Trial (RCT), comparative, and observational study about Tricyclic Antidepressants treatment in chronic LBP from 1998 to 2019. In this review, 5 articles and 543 patients were evaluated. All recruited articles are randomized clinical trials. Among all five relevant articles, three of them evaluate amitriptyline treatment in chronic LBP while the rest articles evaluate imipramine and nortriptyline. Amitriptyline is the most well-studied TCA for chronic LBP. We found that both amitriptyline and nortriptyline can reduce pain severity significantly when the dose is increased gradually depending on patient tolerance. TCAs can also improve disability significantly. However, single-dose imipramine is not superior than placebo. We conclude that TCAs can be effective for treating chronic LBP when used routinely for several weeks and the dose should be increased gradually depending on patient tolerance although further studies are required to confirm the optimal dose for analgesia. The most prevalence Adverse Drug Reactions (ADRs) of TCA including dry mouth, somnolence, insomnia, sedation, and constipation.
Editorial and Mini-Review: Topical Oxygen Therapy for Diabetic Foot Ulcerations - Avenue Towards New Hope?
Authors: Vas Prashanth R.J. , Papanas Nikolaos
Published: January 2020

Diabetic foot complications now represent the 10th leading cause of disease burden and disability. Wound healing is impaired, leading to chronic ulceration. Local high oxygen concentration is required by the metabolically active cells in the wound, which may render the region
hypoxic, even in the absence of peripheral arterial disease. Therefore, the contribution of hyperbaric oxygen to improved healing rates has been extensively investigated. More recent developments include products delivering topical oxygen therapy (TOT) directly at the wound site, either by
continuous delivery or by pressurized systems. A very recent systematic review has found that TOT increases wound healing rates in chronic, less severe diabetic foot ulcers (DFUs), and it promotes high rates of healing in more severe ulcers. Thus, TOT appears to be very promising to improve
healing in DFUs. We now need more experience regarding its therapeutic place in the algorithm of DFU management and in relation to optimal patient selection.
Ege Üniversitesi Hastanesi malign plevral mezotelyoma hastalarının epidemiyolojik ve genel sağ kalım özellikleri
Authors: ERGÖNÜL Ayşe Gül , AKÇAM Tevfik İlker , ÖZDİL Ali , TURHAN Kutsal , ÇAKAN Alpaslan , NART Deniz , ÖZKÖK Serdar , GÖKER Erdem , CANER Ayşe , ÇOK Gürsel , ÇAĞIRICI Ufuk
Published: January 2020

Amaç: Malign plevral mazotelyoma (MPM) kötü prognozlu ölümcül bir hastalıktır. MPM için ortalama sağ kalım 4-18 ay arasında değişmektedir. MPM’de tüm tedavilere rağmen yüksek mortalite görüldüğünden, elimizdeki bilinen tüm tedaviler hastalara uygulanabilmektedir. Bu nedenle tümör evresine, hasta performansına, histolojik alt tipine göre; cerrahi, kemoterapi, radyoterapiyi içeren multimodal tedavi rejimleri söz konusu olmaktadır. Gereç ve Yöntem: Bu çalışmada Ege Üniversitesi Hastanesi veri tabanındaki 1992-2017 yılları arası 241 malign plevral mezotelyoma hastasının epidemiyolojik ve genel sağ kalım özellikleri istatistiksel açıdan global sonuçlarla karşılaştırılacak ve malign plevral mezotelyoma verileri değerlendirilecektir. Bulgular: Kayıtlı toplam 240 hastanın 154’ü (%64) erkek, 86’sı (%36) kadındı. Yas ortalaması 56 (19-78) idi. Sağ kalım değerlendirilmesinde yapılan tedavilerin sağ kalıma etkisine bakıldığında en iyi sonucun (ortalama sağ kalım 24 ay, beş yıllık yaşam %16, p˂0.001) kombine tedavi ile sağlandığı görülmüştür. Patolojik alt grupların sağ kalıma etkisine bakıldığında (Grafik 5) epiteloid mezotelyoma alt tipinin de anlamlı olarak daha iyi yaşam süresi gösterdiği bulunmuştur (ortalama sağ kalım 24 ay, beş yıllık yaşam %15, p
Ege Üniversitesi Hastanesi veri tabanında meme kanseri hastalarının epidemiyolojisi ve genel sağ kalım özellikleri
Authors: HAYDAROĞLU Ayfer , ÇAKAR Burcu , GÖKMEN Erhan , ÖZDEMİR Necmettin , ZEKİOĞLU Osman , ÖZSARAN Zeynep , ALANYALI Senem , GÖKTEPE Berk , YENİAY Levent
Published: January 2020

Amaç: Ege Üniversitesi Hastanesinde 1992-2017 arası kanser tanı ve tedavisi yapılan 13079 meme kanser tanılı hastanın genel özellikleri, tedavi modaliteleri ve sağ kalım sürelerinin tanımlanması amaçlanmıştır.

Gereç ve Yöntem: Ege Üniversitesi Kanserle Savaş Araştırma ve Uygulama Merkezi tarafından toplanan meme kanseri verileri CANREG özel bilgisayar programına kayıt edilmiş, DSÖ ve SEER sistemleri temelinde gruplanarak analizler yapılmıştır. İstatistik analizlerde Ki-kare, General Linear Model, Kaplan Meier sağ kalım analizleri uygulanmıştır. Kaplan Meier Sağ kalım analizinde Log Rank (Mantel-Cox), Breslow (Generalized Wilcoxon) ve Tarone-Ware istatistikleri kullanılmıştır. İstatistik analizlerde p
Ege Üniversitesi Hastanesinde testis kanserlerinin epidemiyolojisi ve genel sağ kalım özellikleri
Authors: GÜRSOY Pınar , ÇAKAR Burcu , GÖKMEN Erhan , SARSIK KUMBARACI Banu , SEMERCİ Bülent , CANER Ayşe , SERT Fatma , ÖZKÖK Serdar , HAYDAROĞLU Ayfer
Published: January 2020

Amaç: Ege Üniversitesi Hastanesinde 1992-Haziran 2018 arası kanser tanısı konan ve tedavi edilen 26 yıllık testis kanseri hastalarının genel özellikleri, tedavi modaliteleri ve sağ kalım sürelerinin tanımlanması amaçlanmıştır. Gereç ve Yöntem: Ege Üniversitesi Kanserle Savaş Araştırma ve Uygulama Merkezi tarafından toplanan testis kanseri verileri CANREG özel bilgisayar programına kaydedilmiş, DSÖ ve SEER sistemleri temelinde gruplanarak analizler yapılmıştır. İstatistik analizlerde Fisher’s Exact Test, Kaplan-Meier sağ kalım analizleri uygulanmıştır. Sağ kalım analizinde Log Rank (Mantel-Cox), Breslow (Generalized Wilcoxon) ve Tarone-Ware istatistikleri kullanılmıştır. İstatistik analizlerde p
O perfuraneve: entre a desesperança e a resistência
Authors: Dobal Susana , Dos Santos Jorge Alam Pereira
Published: January 2020

Em O Perfuraneve uma sociedade confinada em um trem em movimento passa por conflitos e tensões que fazem dessa HQ um reflexo da nossa sociedade real. O artigo investiga como essa ficção científica em forma de história em quadrinho, já adaptada para o cinema e em adaptação para uma série, serve de instrumento de leitura do mundo real mesmo oscilando entre uma postura cética diante de qualquer possibilidade de reação à inevitável opressão social e uma postura mais crítica e engajada. Mesmo que contrárias, a versatilidade da obra torna as duas abordagens legítimas: se por um lado temos um retrato sombrio e preocupante de nosso futuro no planeta, encontramos também personagens e atitudes capazes de inspirar formas de resistência e atuação coletivas. De uma forma ou outra, o Perfuraneve confirma o potencial, nem sempre explorado de que a história em quadrinho pode possuir uma chave de leitura de nossa realidade.
Os quadrinhos italianos pós-77 e o povo porvir: Ranxerox e Squeak the Mouse
Authors: Vargas Alexandre Linck , Marcondes Ciro Inácio
Published: January 2020

Os quadrinhos italianos, a partir do movimento de 77, são marcados pelas experiências das revistas Cannibale e Frigidaire. Criadas por Stefano Tamburini, é nelas que histórias em quadrinhos como RanXerox, de Tamburini e Tanino Liberatore, e Squeak the Mouse, de Massimo Mattioli, são publicadas. Não só esteticamente ligadas às revoltas de 1977, assim como ao seu fracasso revolucionário, essas HQs problematizam o estatuto da modernidade dos quadrinhos, dialogando com as vanguardas artísticas e, ao mesmo passo, instaurando um tempo distinto aos regimes da arte.  Busca-se, portanto, investigar estética e narrativamente nesses quadrinhos italianos pós-77 sua potência mítica, temporalidades heterogêneas e apelo a um povo porvir.
Histórias em quadrinhos e memória: algumas aproximações
Authors: Miorando Guilherme “Smee” Sfredo
Published: January 2020

Os mecanismos que regem a aquisição e a manutenção da memória autobiográfica e da narrativa dos quadrinhos são bastante próximos, para não dizer semelhantes. A memória é um elemento essencial para que as histórias em quadrinhos, bem como sua dimensão física, as revistas em quadrinhos sejam tão populares ao longo de diversas gerações de consumidores ao longo dos anos. Neste breve ensaio teceremos algumas relações que se dão entre conceitos, aplicações e funcionamentos da memória dentro e fora da narrativa dos quadrinhos, mas sempre se relacionando com este tipo de mídia e linguagem. Pensaremos como a aquisição da leitura dos quadrinhos perpassa a memória e como fatores externos à leitura também são importantes para essa experiência mnemônica. Também levaremos em conta os atravessamentos realizados pela memória coletiva na forma com que pensamos os quadrinhos, para, finalmente, construirmos nossas considerações finais sobre essa intrínseca relação. .
A articulação de imagens em quadrinhos e em filmes
Authors: Figueiredo Camila Augusta Pires de
Published: January 2020

O presente estudo se propõe a analisar a potencialidade da mídia dos quadrinhos em comparação com a mídia do cinema, no que se refere ao layout de página/tela. Examinaremos os processos de elaboração e articulação de imagens nas páginas dos quadrinhos e na tela de cinema de acordo com categorização do teórico Thierry Groensteen, um aspecto de extrema importância quando se analisa adaptações de quadrinhos para o cinema. Para isso, apontaremos algumas das possibilidades estéticas no layout da página, bem como alguns dos princípios que os diretores devem ter em mente ao decidir como transpor os quadrinhos para o cinema.
Quadrinhos como instrumentos de crítica ou propaganda política, uma análise das hqs Reagan’s Raiders (1986-1987)
Authors: Pedroso Rodrigo Aparecido Araújo
Published: January 2020

O presente texto tem como objetivo analisar de um ponto de vista historiográfico como o presidente dos Estados Unidos, Ronald Reagan (1911-2004) foi representado na breve série em quadrinhos Reagan’s Raiders. As HQs o apresentam como um super-herói que age para proteger seu país e o mundo de ameaças terroristas, tráfico de drogas e do comunismo. Para tanto, não hesita em invadir outros países e de usar força letal contra aqueles considerados inimigos. A ideia principal é expor as críticas ou sátiras presentes nas HQs e discutir as mensagens que os autores procuraram passar sobre o presidente e o contexto social e político dos EUA em meados dos anos 1980.
Lentidão nos quadrinhos: estratégias de desaceleração da leitura nas narrativas gráficas
Authors: Schneider Greice
Published: January 2020

Desde o início, os quadrinhos modernos parecem ser marcados pela representação do movimento, resultado de uma cultura de velocidade, diretamente relacionada à ligação entre a dimensão seqüencial das narrativas gráficas e as potencialidades do meio na representação visual da progressão. Como contraponto, este artigo defende os quadrinhos como um meio privilegiado para o cultivo de regimes lentos. O desafio aqui é abordar conceitos como ritmo e duração em um meio em que o tempo de leitura não é imposto, mas pode variar amplamente de leitor para leitor. O artigo propõe identificar possíveis estratégias de desaceleração da leitura, como quebra e segmentação dos requadros, densidade da página, quantidade de texto, traço gráfico e a relação entre o nível de detalhe e o grau de atenção exigida. Especial atenção será dedicada ao modo como a relação entre linearidade e tabularidade tão própria dos quadrinhos pode contribuir para explorar a lentidão.
Hak Asasi Perempuan Dalam Islam
Authors: Naamy Nazar
Published: January 2020

Interpretasi tentang HAM serta ayat-ayat yang berkaitan dengan perempuan hanya dipandang sebelah mata dan lebih banyak muatan politik belaka.Kaum perempuan selalu menjadi objek kekerasan dan tidak dipandang sebagai manusia, dimana dalam kelas sosial selalu menjadi objek kekerasan, seperti kekerasan terhadap perkawinan, kejahatan pelacuran, banyaknyajanda-jandayangmasihdibawahumur. Konsep HAMituseolah- olah hanya berlaku bagi kaum laki-laki yang selalu merasa superioritas sedangkan kaum perempuan selalu tersubordinasi. KonsepHAM dalam Islam tentu tidak secara mudah diterima di negara Islam, hal demikian terjadikarena umat Islam selalu memiliki anggapan yang negatif bahwa HAM itu seolah-olah produk dari Barat yangakhirnya berdampakpada kesombongan intelektual serta kemalasan kita untuk mengkaji secara mendalam tentang HAM dalam Islam. Isu apakah HAM ada dalam Islam atau tidak, tentu melahirkan rumusan HAM pada akhir tahun 1948 ketika itu berhasil mencanangkan piagam hak asasi manusia. HAM adalah bagian dari Islam, karena Islam itu sangat universal berlaku untuk segala zaman, dan Islam juga tidak pernah memandang perbedaan antara laki-laki dan perempuan, akan tetapi karena pemahaman individu dan kelompok itulah yang membuat Islam itu menjadi inklusif dan terbatas, sehingga dalam mengartikan konsep HAM masih dipandang sebelah mata dalam dunia Islam. Konsep HAM itu sudah ada dalam tubuh Islam yang dimana dalam sejarah kehidupan dan perjalanan Nabi Muhammad SAW telah mengajarkan kita bahwa tidak ada perbedaan posisi kaum laki-laki dan perempuan, tidak ada diskriminasi satu sama lain dan terlebih lagi dalam konteks sekarang sejak munculnya konsep HAM yang mengusung equality antara laki-laki dan perempuan dimata hukum dan tidak ada diskriminasi antara satu sama lain, karenaIslam sesungguhnya sudah lebih ramah terhadap kaum perempuan.
SEKS PRANIKAH DAN PENANGANANNYA PERSFEKTIF BEHAVIOR DAN KONSELING ISLAM
Authors: Putri Dwi Widarna Lita , Sanaputri Riska
Published: January 2020

Seks pranikah yang terjadi dikalangan remaja saat ini yang semakin hari semakin memprihatinkan. Tujuan dari penelitian ini ialah untuk mengetahui bagaimana penanganan seks pranikah persfektif behavior dan konseling islam yang dilakukan di Desa Tepas Kecamatan Brang Rea Kabupaten Sumbawa Barat. Metode yang digunakan dalam penelitian ini yaitu penelitian kualitatif deskriptif. Artinya data yang dikumpulkan bukan dari berupa angka-angka melainkan data tersebut berasal dari naskah wawancara, catatan lapangan, dokumen pribadi, catatan memo dan dokumen resmi lainnya. Teknik yang digunakan adalah teknik behavior dan konseling islami. Teknik behavior berupa model assesment fungsional, eksposure terapi, asestion traning, dan self management. Sedangkan teknik konseling islami yang digunakan yaitu pendekatan sepritual dan menjalin kasih sayang. Hasilnya dari beberapa teknik diatas, teknik yang sangat berpengaruh dan bisa membuat para konseli atau korban merasa nyaman dan terelepas dari segala beban ialah teknik konseling islam yang pendekatan secara spritual karena para korban merasa lebih dekat dengan Sang Peciptanya.