bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigating the Efficacy and Experiences With Narrative Exposure Therapy in Severe Mentally Ill Patients With Comorbid Post-traumatic Stress Disorder Receiving Flexible Assertive Community Treatment: A Mixed Methods Study
Authors: Mauritz Maria , Goossens Peter , Jongedijk Ruud , Vermeulen Hester , van Gaal Betsie
Published: April 2022

Background

Patients with severe mental illness with repeated interpersonal trauma and post-traumatic stress disorder (PTSD) have a negative illness progression. Traumas are often not treated because of their vulnerability. Narrative exposure therapy (NET) is an effective trauma therapy. It is unknown whether NET is effective and tolerable in these patients receiving community mental healthcare.

Objectives

The objectives of this study are (1) to gain insights into patients’ experiences before, during, and after NET concerning changes in PTSD, dissociative and severe mental ill symptoms, care needs (CAN), quality of life, and global functioning; (2) to identify factors that influence diagnostic changes after NET as compared to patients’ experiences. These insights will help to decide whether NET should be incorporated in usual care for these patients.

Design

A mixed methods convergent design consists of a grounded theory approach with thematic analysis followed by a merged analysis, comparing quantitative, and qualitative data for each participant and by means of a joint matrix.

Participants

Adult psychiatric outpatients (age, 21–65) with post-traumatic stress disorder (PTSD) related to repeated interpersonal trauma were indicted for the study.

Methods

Baseline demographics and clinical characteristics were assessed. Qualitative data were collected 3 months after NET using individual semi-structured in-depth interviews. The merged analysis compared quantitative and qualitative results for each participant.

Results

Twenty-three outpatients (female, 82%) with a mean age of 49.9 years (SD 9.8) participated in the study. Participants experienced NET as intensive, and most of them tolerated it well. Afterward, eighteen participants perceived less symptoms. Mixed analysis showed substantial congruency between quantitative scores and participants’ perceptions of PTSD, dissociative symptoms, and CAN (Cohen’s kappa > 0.4). Remission of PTSD was associated with sufficient experienced support.

Conclusion

Outpatients with severe mental illness underwent intensive NET, and most of them tolerate it well. This therapy is clearly efficacious in this group.

Clinical Trial Registration

[www.ClinicalTrials.gov], identifier [NL5608 (NTR5714)].
Black Perinatal Mental Health: Prioritizing Maternal Mental Health to Optimize Infant Health and Wellness
Authors: Estriplet Tracey , Morgan Isabel , Davis Kelly , Crear Perry Joia , Matthews Kay
Published: April 2022

Infant mental health is interconnected with and affected by maternal mental health. A mother or birthing person's mental health before and during pregnancy and the postpartum period is essential for a child's development. During the first year of life, infants require emotional attachment and bonding to strive. Perinatal mood disorders are likely to hinder attachment and are associated with an increased risk of adverse mental health effects for children later in life. The Black community is faced with a crisis as Black mothers experience a higher prevalence of perinatal mood disorders, including postpartum depression and anxiety, compared to the United States national estimates. The aim of the research is to identify social, structural, and economic disparities of Black perinatal women and birthing people's experience to understand the impact of perinatal mental health on infants' mental health. Black mothers and birthing people may often face social and structural barriers that limit their opportunity to seek and engage with interventions and treatment that address the root causes of their perinatal mood disorder. To enhance understanding of racial disparities caused by social and structural determinants of health on Black mothers and birthing people's mental health and health care experiences that influence infant mental health, the study team conducted semi-structured interviews among self-identified cisgender Black women health professionals nationwide, who provide care to pregnant or postpartum Black women and birthing people. Our study attempted to identify themes, pathways, interventions, and strategies to promote equitable and anti-racist maternal and infant mental health care. Using a Rigorous and Accelerated Data Reduction (Radar) technique and a deductive qualitative analytic approach it was found that limited access to resources, lack of universal screening and mental health education, and the disjointed healthcare system serves as barriers, contribute to mental health issues, and put Black mothers and birthing people at a disadvantage in autonomous decision making. Our study concluded that instituting education on healthy and culturally appropriate ways to support infant development in parent education programs may support Black parents in establishing healthy attachment and bonds. Prioritizing strategies to improve maternal mental health and centering Black parents in developing these educational parenting programs may optimize parenting experiences.
A Dynamic Approach to Economic Priority Setting to Invest in Youth Mental Health and Guide Local Implementation: Economic Protocol for Eight System Dynamics Policy Models
Authors: Lawson Kenny D. , Occhipinti Jo-An , Freebairn Louise , Skinner Adam , Song Yun Ju C. , Lee Grace Yeeun , Huntley Sam , Hickie Ian B.
Published: April 2022

Background

Mental illness costs the world economy over US2.5 Bn each year, including premature mortality, morbidity, and productivity losses. Multisector approaches are required to address the systemic drivers of mental health and ensure adequate service provision. There is an important role for economics to support priority setting, identify best value investments and inform optimal implementation. Mental health can be defined as a complex dynamic system where decision makers are challenged to prospectively manage the system over time. This protocol describes the approach to equip eight system dynamics (SD) models across Australia to support priority setting and guide portfolio investment decisions, tailored to local implementation context.

Methods

As part of a multidisciplinary team, three interlinked protocols are developed; (i) the participatory process to codesign the models with local stakeholders and identify interventions for implementation, (ii) the technical protocol to develop the SD models to simulate the dynamics of the local population, drivers of mental health, the service system and clinical outcomes, and (iii) the economic protocol to detail how the SD models will be equipped to undertake a suite of economic analysis, incorporating health and societal perspectives. Models will estimate the cost of mental illness, inclusive of service costs (health and other sectors, where necessary), quality-adjusted life years (QALYs) lost, productivity costs and carer costs. To assess the value of investing (disinvesting) in interventions, economic analysis will include return-on-investment, cost-utility, cost benefit, and budget impact to inform affordability. Economic metrics are expected to be dynamic, conditional upon changing population demographics, service system capacities and the mix of interventions when synergetic or antagonistic interactions. To support priority setting, a portfolio approach will identify best value combinations of interventions, relative to a defined budget(s). User friendly dashboards will guide decision makers to use the SD models to inform resource allocation and generate business cases for funding.

Discussion

Equipping SD models to undertake economic analysis is intended to support local priority setting and help optimise implementation regarding the best value mix of investments, timing and scale. The objectives are to improve allocative efficiency, increase mental health and economic productivity.
The Feasibility and Utility of Harnessing Digital Health to Understand Clinical Trajectories in Medication Treatment for Opioid Use Disorder: D-TECT Study Design and Methodological Considerations
Authors: Marsch Lisa A. , Chen Ching-Hua , Adams Sara R. , Asyyed Asma , Does Monique B. , Hassanpour Saeed , Hichborn Emily , Jackson-Morris Melanie , Jacobson Nicholas C. , Jones Heather K. , Kotz David , Lambert-Harris Chantal A. , Li Zhiguo , McLeman Bethany , Mishra Varun , Stanger Catherine , Subramaniam Geetha , Wu Weiyi , Campbell Cynthia I.
Published: April 2022

Introduction

Across the U.S., the prevalence of opioid use disorder (OUD) and the rates of opioid overdoses have risen precipitously in recent years. Several effective medications for OUD (MOUD) exist and have been shown to be life-saving. A large volume of research has identified a confluence of factors that predict attrition and continued substance use during substance use disorder treatment. However, much of this literature has examined a small set of potential moderators or mediators of outcomes in MOUD treatment and may lead to over-simplified accounts of treatment non-adherence. Digital health methodologies offer great promise for capturing intensive, longitudinal ecologically-valid data from individuals in MOUD treatment to extend our understanding of factors that impact treatment engagement and outcomes.

Methods

This paper describes the protocol (including the study design and methodological considerations) from a novel study supported by the National Drug Abuse Treatment Clinical Trials Network at the National Institute on Drug Abuse (NIDA). This study (D-TECT) primarily seeks to evaluate the feasibility of collecting ecological momentary assessment (EMA), smartphone and smartwatch sensor data, and social media data among patients in outpatient MOUD treatment. It secondarily seeks to examine the utility of EMA, digital sensing, and social media data (separately and compared to one another) in predicting MOUD treatment retention, opioid use events, and medication adherence [as captured in electronic health records (EHR) and EMA data]. To our knowledge, this is the first project to include all three sources of digitally derived data (EMA, digital sensing, and social media) in understanding the clinical trajectories of patients in MOUD treatment. These multiple data streams will allow us to understand the relative and combined utility of collecting digital data from these diverse data sources. The inclusion of EHR data allows us to focus on the utility of digital health data in predicting objectively measured clinical outcomes.

Discussion

Results may be useful in elucidating novel relations between digital data sources and OUD treatment outcomes. It may also inform approaches to enhancing outcomes measurement in clinical trials by allowing for the assessment of dynamic interactions between individuals' daily lives and their MOUD treatment response.

Clinical Trial Registration

Identifier: NCT04535583.
The Incidence of Catatonia Diagnosis Among Pediatric Patients Discharged From General Hospitals in the United States: A Kids' Inpatient Database Study
Authors: Luccarelli James , Kalinich Mark , Fernandez-Robles Carlos , Fricchione Gregory , Beach Scott R.
Published: April 2022

Objective

Catatonia is a neuropsychiatric condition occurring across the age spectrum and associated with great morbidity and mortality. While prospective cohorts have investigated catatonia incidence among psychiatric patients, no studies have comprehensively explored the incidence of catatonia in general hospitals. We examine the incidence of catatonia diagnosis, demographics of catatonia patients, comorbidities, and inpatient procedures utilized among pediatric patients hospitalized with catatonia in the United States.

Methods

The Kids' Inpatient Database, a national all-payors sample of pediatric hospitalizations in general hospitals, was examined for the year 2019. Hospitalizations with a discharge diagnosis of catatonia were included in the analysis. Hospitalizations with catatonia as the primary discharge diagnosis were compared to hospitalizations with catatonia as a secondary discharge diagnosis.

Results

A total of 900 (95% CI: 850–949) pediatric discharges (291 with catatonia as a primary diagnosis, 609 with catatonia as a secondary diagnosis) occurred during the study year. Mean age was 15.6 ± 2.6 years, and 9.9% were under age 13. Comorbidities were common among patients with catatonia, with psychotic disorders (165; 18.3%), major depressive disorder (69; 7.7%), bipolar disorder (39; 4.3%) and substance-related disorders (20; 2.2%) as the most common primary diagnoses. There was significant comorbidity with neurologic illness, developmental disorders, autism spectrum disorder, and inflammatory conditions. In total 390 catatonia discharges (43.3%) included at least one procedure during admission.

Conclusions

catatonia is rarely diagnosed in pediatric patients in general hospitals but is associated with significant and severe psychiatric and medical comorbidities. Further research is needed into the optimal diagnosis, workup, and treatment of catatonia in pediatric patients.
The Perceptions of Women About Their High Experience of Using Crack Cocaine
Authors: Tractenberg Saulo G. , Schneider Jaluza A. , Mattos Bernardo P. de , Bicca Carla H. M. , Kluwe-Schiavon Bruno , de Castro Thiago G. , Habigzang Luísa F. , Grassi-Oliveira Rodrigo
Published: April 2022

Introduction

The aim of this study was to explore the perceptions of women about their experience in using crack cocaine, discussing their motivations for using it and the repercussions in their lives.

Objective

To investigate these experiences, a qualitative exploratory study was conducted, using the inductive thematic analyses of the content.

Methods

Eight female crack cocaine users took part in this study. They were assessed by a semi-structured interview, addressing the crack cocaine use experience. Four main themes emerged in the interviews: (1) crack cocaine “high” experience; (2) symptoms related to crack cocaine use; (3) circumstances of crack cocaine use; and (4) crack cocaine use consequences.

Results

The main perceptions reported by the users were related to a feeling of being disconnected to the world preceded by a pleasant experience, especially during the first moments of use. They revealed that the drug fulfills a key role of coping strategy to handle with negative thoughts, emotions or life experiences. An important influence of social issues was reported in relation to the onset of crack cocaine use. Negative consequences and significant impact on their lives appeared in their reports, regarding the loss of family ties, involvement with prostitution, traumatic experiences and violence.

Conclusion

Taking together all women's perceptions suggests that beyond the positive immediate rewarding effect, the maintenance of use might be related to the dissociative experience and self-medication role, acting as negative reward by relieving of negative life experiences that, in turn, are both cause and consequence of the drug use.
Arsenic in Drinking Water and Incidences of Leukemia and Lymphoma: Implication for Its Dual Effects in Carcinogenicity
Authors: Lin Ming-Hsien , Li Chung-Yi , Cheng Ya-Yun , Guo How-Ran
Published: April 2022

Arsenic in drinking water has been recognized as carcinogenic to humans and can cause solid cancers of lung, urinary bladder, and skin. Positive associations have also been reported between arsenic ingestion and cancers of kidney, liver and prostate. Nevertheless, arsenic trioxide has been used successfully in the treatment of acute promyelocytic leukemia. Therefore, arsenic might play different roles in the carcinogenesis of solid cancers and hematologic malignancies. The relationship between arsenic in drinking water and the incidences of hematologic malignancies has not been fully investigated. We established a cohort of Taiwanese population and assorted 319 townships of Taiwan into two exposure categories using 0.05 mg/L as the cutoff. Then, we linked these data to the Taiwan Cancer Registry and computed standardized incidence ratios (SIRs) of lymphoma and leukemia by sex, exposure category and time period. The trend of changes in the SIRs over time was assessed, from 1981–1990 to 1991–2000 and then to 2001–2010. We found that in both lymphoma and leukemia, the higher exposure category was associated with lower SIRs in both men and women. In terms of time trends, the SIRs in both lymphoma and leukemia showed increasing trends in both sexes, while exposure to arsenic in drinking water decreased over time. The arsenic level in drinking water was negatively associated with the incidences of lymphoma and leukemia in both men and women. This study supports the dual effects of arsenic on carcinogenesis, with a potential protective effect against hematologic malignancies.
It Is “All About Relationships” in Lifestyle Programmes for Adults Living With Type Two Diabetes Underpinned by a Person/Whānau-Centred Care Approach
Authors: Hale Leigh , Higgs Christopher , Keen Donna , Smith Catherine
Published: April 2022

Background

Lifestyle programmes are important in the management of type 2 diabetes (T2D). The Diabetes Community Exercise Programme (DCEP) is an exercise and educational programme for adults living with T2D with the aim of enhancing exercise self-efficacy and supporting wellbeing. DCEP is underpinned by a model of person/whānau-centred care and the spirit of Motivational Interviewing. Person-centred care models in the context of rehabilitation and long-term health conditions are still evolving. This paper explores what those involved in DCEP perceived important to its person/whānau-centredness.

Method

An evaluative qualitative methodological approach was used with data collected by open-ended interviews and a focus-group at completion of the initial 12-week part of DCEP. Interviews were audio-recorded and transcribed verbatim. Participants were 16 DCEP attendees and 13 healthcare professionals (HCPs) involved in DCEP delivering. Data were analysed using the General Inductive Approach.

Results

The three interconnected themes constructed from the analysis were ‘It's all about relationships’, Balancing the Outside World, and Empowering through Knowledge. Through the experience and motivation of positive person-centred relationships, DCEP addressed attendees' T2D health needs, but they were constantly balancing these needs with those of family, employers, finances, other health needs and life interests. DCEP relationships facilitated ongoing discussions between attendees and between attendees and HCPs. The ability to discuss in a supportive and non-judgmental environment helped attendees to make sense and process the information they gained at DCEP. This empowering through knowledge in turn helped attendees to work out ways of balancing the outside world and thus better address their T2D needs.

Conclusion

It is “all about relationships” was key to DCEP's person/whānau-centred approach—all other factors related back to the development and maintenance of relationships. These relationships were between all whānau involved: the attendees, their family, the wider community, the healthcare provider organisations, and the HCPs and personnel involved in delivering DCEPs. These relationships created an accepting, understanding and social atmosphere that enabled attendance and facilitated both knowledge exchange and ability to exercise, both considered to be beneficial by attendees. Importantly, these relationships took time to develop, but the benefits were worth the investment.
An Overview of the MPEG Standard for Storage and Transport of Visual Volumetric Video-Based Coding
Authors: Ilola Lauri , Kondrad Lukasz , Schwarz Sebastian , Hamza Ahmed
Published: April 2022

The increasing popularity of virtual, augmented, and mixed reality (VR/AR/MR) applications is driving the media industry to explore the creation and delivery of new immersive experiences. One of the trends is volumetric video, which allows users to explore content unconstrained by the traditional two-dimensional window of director’s view.

The ISO/IEC joint technical committee 1 subcommittee 29, better known as the Moving Pictures Experts Group (MPEG), has recently finalized a group of standards, under the umbrella of Visual Volumetric Video-based Coding (V3C). These standards aim to efficiently code, store, and transport immersive content with 6 degrees of freedom. The V3C family of standards currently consists of three documents: 1) ISO/IEC 23090-5 defines the generic concepts of volumetric video-based coding and its application to dynamic point cloud data; 2) ISO/IEC 23090-12 specifies another application that enables compression of volumetric video content captured by multiple cameras; and 3) ISO/IEC 23090-10 describes how to store and deliver V3C compressed volumetric video content. Each standard leverages the capabilities of traditional 2D video coding and delivery solutions, allowing for re-use of existing infrastructures which facilitates fast deployment of volumetric video.

This article provides an overview of the generic concepts of V3C, as defined in ISO/IEC 23090-5. Furthermore, it describes V3C carriage related functionalities specified in ISO/IEC 23090-10 and offers best practices for the community with respect to storage and delivery of volumetric video.
Neoadjuvant Systemic Therapy in Localized and Locally Advanced Renal Cell Carcinoma
Authors: Khaleel Sari , Jiang Song , Kotecha Ritesh R. , Hakimi A. Ari
Published: April 2022

While the majority of renal cell carcinoma (RCC) cases present at an early stage, a significant number of patients are diagnosed with either locally advanced or metastatic disease. While surgical resection remains the definitive curative management in the localized setting, many patients experience disease relapse and the 5-year recurrence rate following nephrectomy nears 60% for patients with high-risk localized disease. As systemic therapies including anti-angiogenesis, immune checkpoint blockade, and combinations thereof have evolved with dramatic improvements in survival outcomes for patients with metastatic RCC, there is a renewed interest in exploring the utility of these agents in the upfront neoadjuvant and adjuvant setting. Neoadjuvant therapy, administered prior to definitive surgery, aims to eradicate micro-metastatic disease early on and reduce surgical complexity with the overall goals of lowering perioperative morbidity and increasing post-operative recurrence-free and progression-free survival. In this chapter, we present an overview of previously completed and ongoing neoadjuvant systemic therapy clinical trials for patients with localized and locally advanced RCC and discuss potential considerations regarding the utility and future study of neoadjuvant therapy for the optimal management of localized RCC.
Application Research of Three-Dimensional Printing Technology and Three-Dimensional Computed Tomography in Segmentectomy
Authors: Tongxin Li , Jing Xu , Runyuan Wang , Wei Wu , Yu Zhou , Dong Wang , Wang He , Yi Wu , Ping He , Yong Fu
Published: April 2022

Background

To compare the application of the emerging 3D printing technology and 3D-CT in segmentectomy. And to explore the advantages of 3D printing technology in thoracoscopic segmentectomy.

Methods

We collected the clinical data of 118 patients undergoing thoracoscopic segmentectomy from January 2019 to April 2021 at the Thoracic Surgery Department, the Dianjiang People's Hospital of Chongqing and Southwest Hospital. Among them, 61 patients were in the 3D printing group and 57 patients were in the 3D-CT group respectively. The perioperative data of these two groups of patients were analyzed respectively.

Results

There were no significant differences between the two groups in age, gender, tumor diameter, pathology, the preoperative complications of diabetes and heart disease. However, the patients with the complications of hypertension in the 3D printing group are significantly more than the 3D-CT group (P = 0.003). Compared with the 3D-CT group, patients in the 3D printing group had significantly shorter operation time (162.7 ± 47.0 vs. 190.3 ± 56.9 min, P = 0.006), less intraoperative fluid input (1,158.5 ± 290.2 vs. 1,433.2 ± 653.3, P = 0.013), and less total intraoperative fluid output, including intraoperative blood loss, urine excretion, and other fluid loss. In addition, there were no statistically significant differences in intraoperative blood loss, 24 h pleural fluid volume, 48 h pleural fluid volume, postoperative chest tube duration, postoperative hospital stay and complications between the two groups of patients (P > 0.05).

Conclusions

In thoracoscopic segmentectomy, the application of 3D printing technology shortens the operation time, reduces intraoperative fluid input and output, guides the operation more safely and effectively, and has better clinical application value.
A Metataxonomic Analysis of Maple Sap Microbial Communities Reveals New Insights Into Maple Syrup Complexity
Authors: N’guyen Guillaume Quang , Roblet Cyril , Lagacé Luc , Filteau Marie
Published: April 2022

Maple syrup, an emblematic food product of Canada is produced from the concentration of sap collected from maple trees during spring. During this season, the trees come out of dormancy, which modifies sap composition. Meanwhile, microorganisms that contaminate sap as it is collected can also modify its composition. As these two factors can impact the quality of maple syrup, we aimed to better understand how microbial communities vary along dormancy release. We estimated the absolute abundance of bacteria and fungi in maple sap along a dormancy release index using high-throughput amplicon sequencing and digital droplet PCR (ddPCR). Several members were identified as indicators of maple sap composition, syrup organoleptic conformity and color, some of which are also hubs in the microbial association networks. We further explored bacterial communities by performing a predictive functional analysis, revealing various metabolic pathways correlated to dormancy release. Finally, we performed an experimental investigation of maple sap carrying capacity and limiting nutrients along dormancy release and found that maple sap composition variation influences its carrying capacity. Taken together, our results indicate that an increase in nitrogen supply in the form of allantoate combined with possible metabolite excretion could lead microbial communities towards different paths. Indeed, we observed a greater heterogeneity during late dormancy release which in turn could explain the variation in maple syrup quality. Further experimental investigation into the contribution of microbial, vegetal, environmental, technological, and processing factors to the final composition of maple syrup will be required to improve our understanding of this complex and flavorful food matrix and to develop quality control strategies.
Potential Seeding From Fine-Needle Aspiration of an Axial Osteosarcoma: A Case Report
Authors: Faletti Tasha , Seguin Bernard , Selmic Laura Elizabeth , Lapsley Janis , Worley Deanna , Griffin Maureen , Tremolada Giovanni
Published: April 2022

This report describes the first potential case of seeding after fine-needle aspiration (FNA) of a rib osteosarcoma in a dog. An 8-year-old, 28-kg female spayed Golden Retriever was presented to her primary veterinarian with a 3-week history of a 3-cm firm, unpainful, immobile mass arising from the 9th rib. The mass was aspirated and submitted for cytological examination. A subcutaneous nodule developed several days after the FNA was performed in a location immediately overlying but distinct from the primary rib tumor on palpation. Both the primary mass and the newly diagnosed subcutaneous nodule were biopsied and were consistent with an osteosarcoma. Although it cannot be ruled out that the subcutaneous lesion was metastatic, seeding was a reasonable explanation based on where the new mass was located and how quickly it appeared after the FNA was performed. The aim of this case report was to describe the possibility of tumor seeding during FNA for osteosarcoma. It is the authors' opinion that utility of cytological diagnosis of bone tumors outweighs the risk of possible seeding and should continue to be used as a routine diagnostic test for the diagnosis of aggressive bone lesions.
Risk Factor Assessment of the Smallholder Baby Vegetable Production in Eswatini
Authors: Dlamini Daisy Delsile , Zuwarimwe Jethro , Francis Joseph , Mchau Godwin R. A.
Published: April 2022

The transition from subsistence to commercial production poses uncertainty and risks that require putting in place adaptive systems. Such systems are imperative for agricultural production where weather and food markets are always changing. Disregarding risk factors has a cumulative negative impact on the intended outcomes. The marginality of smallholder farmers’ operational environment renders them limited to strive without government interventions. Research has proven that smallholder farmers face numerous challenges in vegetable value chains. The study sought to identify and characterise smallholder growers risk factors when producing for this value chain. A sequential mixed-method research design was adopted, and data collected from fifty-eight growers from three producer groups in the Manzini and Hhohho regions. Discriminant analysis validated the heterogeneity of respondents using the experience on risk factors. The growers were significantly affected by market and production risks. The clustered growers had a high probability of losses in the value of the harvest whereas the private growers had a high probability of yield changes. Fruit size and postharvest handling contributed to changes in the value of the harvest. An improved cold chain system could enable symmetry in the harvest scheduling and grade information as well as prompt payments. Interventions toward enabling access and use of quality farm inputs would curb yield variations. Future research could quantify yield losses at each stage of postharvest handling toward informing the risk management strategy.
Economic Viability of Ultrasonic Sensor Actuated Nozzle Height Control in Center Pivot Irrigation Systems
Authors: AL-agele Hadi A. , Mahapatra Durga Madhab , Nackley Lloyd , Higgins Chad
Published: April 2022

The coverage of center pivot irrigation systems used around the world has increased. One potential factor driving their adoption is improved water application efficiency relative to some other sprinkler or surface irrigation approaches. Center pivot irrigation systems may be further improved by dynamic elevation spray application (DESA). DESA systems adjust the nozzle height in response to plant growth and canopy heterogeneities. The DESA approach is relatively new and there is uncertainty in its economic viability and worthiness of further investigation. Thus, an economic scenario analysis was performed to explore the potential economic benefits of DESA based on permutations of irrigation pivot efficiency without DESA, water-saving potential of DESA, and water cost. The weighted costs and benefits of the height-adjusted approach for a set of water cost savings scenarios showed the net return price with the water cost savings per season. We show that DESA could have economic viability at current component costs and is worthy of further investigation and refinement.
Ex Vitro Simultaneous Acclimatization and Rooting of In Vitro Propagated Tamarillo Plants (Solanum betaceum Cav.): Effect of the Substrate and Mineral Nutrition
Authors: Salgado Pirata Madalena , Correia Sandra , Canhoto Jorge
Published: April 2022

Plants propagated by seed do not ensure genetic uniformity and are sometimes infected with diseases. In Vitro micropropagation techniques are an alternative method to traditional cloning approaches for producing true-to-type and pathogen-free plants. However, due to the particularities of the in vitro environment, these plants face many challenges, often critical to their survival, to adapt to ex vitro conditions. In this context, four substrates and two types of mineral nutrition (quick-release (QRF) and controlled-release (CRF) fertilizers), as well as their absence were evaluated in the process of acclimatization of Solanum betaceum plants. Stomatal conductance (gs), chlorophyll content index (CCI), and dry biomass of roots, shoots, and entire plants were the parameters analyzed during the acclimatization. The best crop performance (gs, CCI, and dry biomass) were observed in substrates consisting of vermiculite plus the application of mineral nutrients through a CRF, proving that mineral nutrition has the greatest positive impact on the acclimatization process. In these conditions, plants were obtained with a total dry biomass being significantly higher (515.0 mg (QRF) and 635.9 mg (CRF) when compared to the total dry biomass of untreated plants (119.9 mg). The best conditions for this first experiment were replicated in a second test in order to assess the best fertilizer amount suited for plantlet growth. In this case, the best results were obtained with 0.4 g of CRF, in which plants showed a dry biomass of roots (542.7 mg) and a total dry biomass (594.5 mg), which was significantly higher than in the control (183.2 mg and 165.9 mg, respectively) or with other concentrations of CRF (0.8 and 1.6 g). A similar trend was found concerning the CCI (5.3) and gs (72.5 mmol m−2 s−1) in which 0.4 g CRF gave also the best results when compared with the control (without CRF) or with 0.8 g (4.7 and 56.2 mmol m−2 s−1) and 1.6 g (4.7 and 52.2 mmol m−2 s−1) treatments. In general, it was found that tamarillo plantlets acclimatized to 0.4 g of CRF had a faster initial growth and better performance (CCI and gs), with plants ready to go to the greenhouse/field more quickly, thus reducing the time to obtain suitable plants for the market and shortening the production cycle.
Temporal Variation of the Non-Volatile Compounds and Key Odorants in Xinyang Maojian Green Teas during the Spring and Autumn Seasons
Authors: Yin Peng , Kong Ya-Shuai , Liu Pan-Pan , Jiang Chang-Ling , Sun Mu-Fang , Guo Gui-Yi , Liu Zhong-Hua
Published: April 2022

Xinyang Maojian (XYMJ) green tea is one of the top ten teas in China, and the consumers prefer spring tea due to its umami taste and pleasurable aroma. However, the knowledge about temporal variation of the volatile compounds in XYMJ green teas harvested during different seasons is very limited. In the present work, the main non-volatile compounds that endowed the taste and volatile compounds responsible for the aroma in XYMJ green teas harvested during the spring and autumn seasons were determined. The average contents of free amino acids (FAA) were significantly higher and gradually declined in the spring teas, whereas the caffeine was significantly lower and gradually increased in the spring teas. A total of 39 volatile compounds of six chemical classes were detected in XYMJ green teas, and they displayed various change trends during the spring and autumn seasons, among which 15 volatile compounds were identified as the key odorants based on odor activity value (OAV). The highest OAV of 2195.05 was calculated for the violet-like smelling trans-β-ionone followed by decanal, nonanal, dimethyl sulfide, linalool, geraniol and naphthalene. The OAVs of geraniol, (Z)-3-hexenyl hexanoate, heptanal, benzaldehyde and hexanal in XYMJ spring teas were higher than XYMJ autumn teas. The hierarchical clustering analysis indicated that XYMJ green teas were divided into three clusters and the quality of XYMJ green teas changed greatly within spring season. Harvest season is a crucial factor affecting the flavor quality of XYMJ green teas.
Horse Racing as a Model to Study the Relationship between Air Pollutants and Physical Performance
Authors: Araneda Oscar F.
Published: April 2022

This study proposes the theoretical principles for the selection of a sample of horse races to study the relationship between air pollutants and performance. These criteria were then applied to an original dataset comparing the correlations between these variables obtained in “Handicap” versus “Conditional” type races. Methods: The mean concentration of pollutants during the six hours prior to the race and the speed of the test were determined in 441 official races at a racecourse in Santiago, Chile, during the summer and winter months of 2012. Using layout, track condition and distance (1000, 1100 and 1200 m) as criteria, a homogeneous group of races (“Handicap”; n = 214) versus a heterogeneous group (“Conditional”; n = 95) were compared using simple correlations (Spearman’s test). Results: Race speed was related to greater levels of PM10, PM2.5, NO2, NO and SO2 and it was positively related to O3, a trend that was observed in the 1000, 1100 m races and in the total “Handicap” group. Similar results were observed only in 1000 m for the “Conditional” group with lower Rho, except for PM10 and PM2.5. The total races of the conditional group showed lower Rho values and significant associations of the same trend for CO, NO2, NO and SO2. Conclusions: Horse races between 1000 and 1200 m of the “Handicap” type appear to be an interesting group to study the relationship between air pollutants and the performance of racehorses. In the future, our observations should be expanded to other distances and other types of races.
Nesting Site and Plumage Color Are the Main Traits Associated with Bird Species Presence in Urban Areas
Authors: Leveau Lucas M. , Ibáñez Isis
Published: April 2022

Urban areas are expected to grow in the next decades, filtering bird species from the regional pool based on their life history traits. Although the impact of urbanization on traits such as diet, habitat and migratory behavior has been analyzed, their joint role with other traits related to plumage color has not yet been analyzed. Urban characteristics such as impervious surfaces, human presence and pollutants may be related to dark and uniform plumages. The objective of this study is to determine different bird species responses to urbanization using ordination analysis, and to characterize their life history traits combining information about diet, habitat and plumage color. Birds were surveyed along urban–rural gradients located in three cities of central Argentina. Species associations with urban characteristics were assessed through principal component analysis. Two axes were obtained: the first related positively to urban exploiters and negatively to urban avoiders, and a second axis related negatively to urban adapters. The scores of each axis were related to species traits through phylogenetic generalized least squares models. Species identified as ‘urban exploiters’ tended to nest in buildings and have uniform plumage, whereas those identified as ‘urban avoiders’ tended to be ground-nesting species with variable plumage. A third type, ‘urban adapters’, tended to be tree-nesting species with a low diet breadth, intermediate plumage lightness, low presence of plumage sexual dimorphism and high presence of iridescence. The results suggest that nest predation and habitat loss may exclude ground nesting birds from urban areas. The high density of pedestrians and domestic animals, such as cats and dogs, in urban centers may favor uniform plumages in birds that enhance camouflage.
Molecular Characterization of Herpesviral Encephalitis in Cetaceans: Correlation with Histopathological and Immunohistochemical Findings
Authors: Sierra Eva , Fernández Antonio , Fernández-Maldonado Carolina , Sacchini Simona , Felipe-Jiménez Idaira , Segura-Göthlin Simone , Colom-Rivero Ana , Câmara Nakita , Puig-Lozano Raquel , Rambaldi Anna Maria , Suárez-Santana Cristian , Arbelo Manuel
Published: April 2022

Herpesviruses are causative agents of meningitis and encephalitis in cetaceans, which are among the main leading known natural causes of death in these species. Brain samples from 103 stranded cetaceans were retrospectively screened for the presence of herpesvirus DNA in the brain. Molecular detection of Cetacean Morbillivirus was performed in HV positive brain cases. Histopathologic evaluation of brain samples included the presence or absence of the following findings (n = 7): meningitis, perivascular cuffings, microgliosis, intranuclear inclusion bodies, malacia, neuronal necrosis and neurophagic nodules, and haemorrhages. Histological evidence of the involvement of other etiological agents led to complementary analysis. We detected the presence of alpha and gamma-HVs in 12 out of 103 (11.6%) brain samples from stranded cetaceans of five different species: one bottlenose dolphin, six striped dolphins, three Atlantic spotted dolphins, one Cuvier’s beaked whale, and one common dolphin. Pathogenic factors such as viral strain, age, sex, and the presence of co-infections were analysed and correlated with the brain histopathological findings in each case. Herpesvirus was more prevalent in males, juveniles, and calves and a 41.6% incidence of co-infections in the brain was detected in our study: three with Dolphin Morbillivirus, one with Staphilococcus aureus septicaemia and one with Brucella spp.
Modeling Paratuberculosis Transmission in a Small Dairy Herd Typical of Slovenia Suggests That Different Models Should Be Used to Study Disease Spread in Herds of Different Sizes
Authors: Knific Tanja , Kirbiš Andrej , Gethmann Jörn M. , Prezelj Jasna , Krt Branko , Ocepek Matjaž
Published: April 2022

This study aimed to investigate the possible dynamics of paratuberculosis or Johne’s disease in a typical Slovenian dairy herd of about 17 cows. Paratuberculosis is a worldwide endemic disease of cattle caused by Mycobacterium avium subsp. paratuberculosis (MAP) and is associated with significant economic losses. We developed a stochastic compartmental model with two pathways of disease progression, infections of adult cows and infections of young animals through horizontal and vertical transmission, and transmission through animal movements. The average proportions of subclinically and clinically infected cows were 4% and 0.47%, respectively. The prevalence within the herd, which included latently infected animals, averaged 7.13% and ranged from 0% to 70.59%. Under the given circumstances, the results showed a relatively high rate of spontaneous elimination (0.22 per herd per year) of the disease and a high rate of reinfection (0.18 per herd per year) facilitated by active animal trade. To our knowledge, this stochastic compartmental model is the first to be developed specifically to represent a small dairy herd and could apply to other countries with a similar structure of dairy farms. The results suggest that different models should be used to study MAP spread in herds of various sizes.
Is There an Association between Paw Preference and Emotionality in Pet Dogs?
Authors: Simon Tim , Frasnelli Elisa , Guo Kun , Barber Anjuli , Wilkinson Anna , Mills Daniel S.
Published: April 2022

Research with humans and other animals has suggested that preferential limb use is linked to emotionality. A better understanding of this still under-explored area has the potential to establish limb preference as a marker of emotional vulnerability and risk for affective disorders. This study explored the potential relationship between paw preference and emotionality in pet dogs. We examined which paw the dogs preferentially used to hold a Kong™ and to perform two different locomotion tests. Dogs’ emotionality was assessed using a validated psychometric test (the Positive and Negative Activation Scale—PANAS). Significant positive correlations were found for dogs’ paw use between the different locomotion tasks, suggesting that dogs may show a more general paw preference that is stable across different types of locomotion. In comparison, the correlations between the Kong™ Test and locomotion tests were only partially significant, likely due to potential limitations of the Kong™ Test and/or test-specific biomechanical requirements. No significant correlations were identified between paw preference tests and PANAS scores. These results are in contrast to previous reports of an association between dog paw preference and emotionality; animal limb preference might be task-specific and have variable task-consistency, which raises methodological questions about the use of paw preference as a marker for emotional functioning.
Weighted Single-Step Genome-Wide Association Study Uncovers Known and Novel Candidate Genomic Regions for Milk Production Traits and Somatic Cell Score in Valle del Belice Dairy Sheep
Authors: Mohammadi Hossein , Farahani Amir Hossein Khaltabadi , Moradi Mohammad Hossein , Mastrangelo Salvatore , Di Gerlando Rosalia , Sardina Maria Teresa , Scatassa Maria Luisa , Portolano Baldassare , Tolone Marco
Published: April 2022

The objective of this study was to uncover genomic regions explaining a substantial proportion of the genetic variance in milk production traits and somatic cell score in a Valle del Belice dairy sheep. Weighted single-step genome-wide association studies (WssGWAS) were conducted for milk yield (MY), fat yield (FY), fat percentage (FAT%), protein yield (PY), protein percentage (PROT%), and somatic cell score (SCS). In addition, our aim was also to identify candidate genes within genomic regions that explained the highest proportions of genetic variance. Overall, the full pedigree consists of 5534 animals, of which 1813 ewes had milk data (15,008 records), and 481 ewes were genotyped with a 50 K single nucleotide polymorphism (SNP) array. The effects of markers and the genomic estimated breeding values (GEBV) of the animals were obtained by five iterations of WssGBLUP. We considered the top 10 genomic regions in terms of their explained genomic variants as candidate window regions for each trait. The results showed that top ranked genomic windows (1 Mb windows) explained 3.49, 4.04, 5.37, 4.09, 3.80, and 5.24% of the genetic variances for MY, FY, FAT%, PY, PROT%, and total SCS, respectively. Among the candidate genes found, some known associations were confirmed, while several novel candidate genes were also revealed, including PPARGC1A, LYPLA1, LEP, and MYH9 for MY; CACNA1C, PTPN1, ROBO2, CHRM3, and ERCC6 for FY and FAT%; PCSK5 and ANGPT1 for PY and PROT%; and IL26, IFNG, PEX26, NEGR1, LAP3, and MED28 for SCS. These findings increase our understanding of the genetic architecture of six examined traits and provide guidance for subsequent genetic improvement through genome selection.
Fluorescence Spectroscopy for the Diagnosis of Endometritis in the Mare
Authors: D’Agostino Andrea , Di Palma Tommaso , Cecchini Gualandi Stefano , Boni Raffaele
Published: April 2022

By exploiting the PMN property to produce high quantities of oxygen peroxide to neutralize pathogens, the oxygen peroxide content of uterine cells was measured to diagnose endometritis. After preliminary in vitro studies in which endometrial cells from slaughtered mares were mixed with leukocytes from peripheral blood, endometrial samples were collected by uterine flushing from mares before insemination. Staining endometrial cells with H2DCF-DA was combined with hydroethidine to normalize the fluorescence intensity with the cellular content of the sample. Stained cell smears were assumed as the gold standard of endometritis, and based on this assay, the samples were considered positive (C+) and negative (C−) for endometritis. The amount and the turbidity of fluid recovered by uterine flushing were significantly (p < 0.01) higher in C+ than in C−. Moreover, the oxygen peroxide content of the endometrial cells was significantly higher in the C+ than in the C− group (6.31 ± 1.92 vs. 3.12 ± 1.26, p = 0.001). Using the value of 4.4 as the cutoff level of this fluorescence cytology assay, it was found that only one C− sample exceeded the cutoff level (false positives = 7.7%) while three C+ samples showed values below the cutoff level (false negative = 11.5%).