bluuebunny/crossref_metadata_2025 huggingface dataset:
An Inexpensive CRISPR-Based Point-of-Care Test for the Identification of Meat Species and Meat Products
Authors: Tao Dagang , Xiao Xiao , Lan Xiaochen , Xu Bingrong , Wang Yuan , Khazalwa Emmanuel Mulaya , Pan Wenya , Ruan Jinxue , Jiang Yu , Liu Xiangdong , Li Changchun , Ye Ruizhen , Li Xinyun , Xu Jing , Zhao Shuhong , Xie Shengsong
Published: May 2022

The growing demand for and supply of meat and meat products has led to a proportional increase in cases of meat adulteration. Adulterated meat poses serious economic and health consequences globally. Current laboratory methods for meat species identification require specialized equipment with limited field applications. This study developed an inexpensive, point-of-care Loop-Mediated Isothermal Amplification (LAMP)-CRISPR/Cas12a colorimetric assay to detect meat species using a Texas Red-labelled single-strand (ssDNA) reporter. As low as 1.0 pg/µL of the porcine NADH4, the chicken NADH dehydrogenase subunit 2 (ND2) and the duck D-loop genes was detectable under white, blue and ultraviolet light. The test turnaround time from DNA extraction to visualization was approximately 40 min. The assay accurately detected pure and mixed-meat products in the laboratory (n = 15) and during a pilot point-of-care test (n = 8) in a food processing factory. The results are 100% reproducible using lateral flow detection strips and the real-time PCR detection instrument. This technology is fully deployable and usable in any standard room. Thus, our study demonstrates that this method is a straightforward, specific, sensitive, point-of-care test (POCT) adaptable to various outlets such as customs, quarantine units and meat import/export departments.
RETRACTED: RADseq Data Suggest Occasional Hybridization between Microcebus murinus and M. ravelobensis in Northwestern Madagascar
Authors: Teixeira Helena , van Elst Tobias , Ramsay Malcolm , Rakotondravony Romule , Salmona Jordi , Yoder Anne , Radespiel Ute
Published: May 2022

The occurrence of natural hybridization has been reported in a wide range of organisms, including primates. The present study focuses on the endemic lemurs of Madagascar, primates for which only a few species occur in sympatry or parapatry with congeners, thereby creating limited opportunity for natural hybridization. This study examines RADseq data from 480 individuals to investigate whether the recent expansion of Microcebus murinus towards the northwest and subsequent secondary contact with Microcebus ravelobensis has resulted in the occurrence of hybridization between the two species. Admixture analysis identified one individual with 26% of nuclear admixture, which may correspond to an F2- or F3-hybrid. A composite-likelihood approach was subsequently used to test the fit of alternative phylogeographic scenarios to the genomic data and to date introgression. The simulations yielded support for low levels of gene flow (2Nm0 = 0.063) between the two species starting before the Last Glacial Maximum (between 54 and 142 kyr). Since M. murinus most likely colonized northwestern Madagascar during the Late Pleistocene, the rather recent secondary contact with M. ravelobensis has likely created the opportunity for occasional hybridization. Although reproductive isolation between these distantly related congeners is not complete, it is effective in maintaining species boundaries.
A Fruit Colour Development Index (CDI) to Support Harvest Time Decisions in Peach and Nectarine Orchards
Authors: Scalisi Alessio , O’Connell Mark G. , Islam Muhammad S. , Goodwin Ian
Published: May 2022

Fruit skin colour is one of the most important visual fruit quality parameters driving consumer preferences. Proximal sensors such as machine vision cameras can be used to detect skin colour in fruit visible in collected images, but their accuracy in variable orchard light conditions remains a practical challenge. This work aimed to derive a new fruit skin colour attribute—namely a Colour Development Index (CDI), ranging from 0 to 1, that intuitively increases as fruit becomes redder—to assess colour development in peach and nectarine fruit skin. CDI measurements were generated from high-resolution images collected on both east and west sides of the canopies of three peach and one nectarine cultivars using the commercial mobile platform Cartographer (Green Atlas). Fruit colour (RGB values) was extracted from the central pixels of detected fruit and converted into a CDI. The repeatability of CDI measurements under different light environments was tested by scanning orchards at different times of the day. The effects of cultivar and canopy side on CDI were also determined. CDI data was related to the index of absorbance difference (IAD)—an index of chlorophyll degradation that was correlated with ethylene emission—and its response to time from harvest was modelled. The CDI was only significantly altered when measurements were taken in the middle of the morning or in the middle of the afternoon, when the presence of the sun in the image caused significant alteration of the image brightness. The CDI was tightly related to IAD, and CDI values plateaued (0.833 ± 0.009) at IAD ≤ 1.20 (climacteric onset) in ‘Majestic Pearl’ nectarine, suggesting that CDI thresholds show potential to be used for harvest time decisions and to support logistics. In order to obtain comparable CDI datasets to study colour development or forecast harvest time, it is recommended to scan peach and nectarine orchards at night, in the early morning, solar noon, or late afternoon. This study found that the CDI can serve as a standardised and objective skin colour index for peaches and nectarines.
The Effect of Child Abuse and Neglect on Trajectories of Depressive Symptoms and Aggression in Korean Adolescents: Exploring Gender Differences
Authors: Choe Chung , Yu Seunghee
Published: May 2022

We analyzed gender differences in the effects of child abuse and neglect experienced during adolescence on depressive symptoms and aggression in Korean adolescents using a representative sample of participants over a three-year period. We applied a latent growth model to a sample of 1797 adolescents aged 14–16 from the Korean Children and Youth Panel Survey. Our findings revealed that abuse increased depressive symptoms in early adolescence, while lowering the rate of increase in depressive symptoms over time. Neglect adversely affected depressive symptoms in boys, but not in girls. Abuse increased the initial value of aggression in girls more than in boys, but reduced the increase rate of aggression over time in girls. Neglect increased the initial value of aggression only in boys. Consequently, abuse and neglect experienced during adolescence can affect depressive symptoms and aggression in the individual differently, depending on gender. This study suggests that, in order to reduce depressive symptoms and aggression in adolescents, work should be undertaken to solve the problems of abuse and neglect, and different approaches should be taken according to the gender of the individual.
Physical Activity, Healthy Behavior and Its Motivational Correlates: Exploring the Spillover Effect through Stages of Change
Authors: Marentes-Castillo María , Castillo Isabel , Tomás Inés , Alvarez Octavio
Published: May 2022

On the basis of the spillover or transfer effect and the transtheoretical model of change, this study assessed the association between amount of physical activity, healthy and unhealthy weight control behaviors, and motivational types, as well as their variability across stages of change. A total of 1219 randomly selected Mexican adults from 18 to 65 years old, representative of the city of Monterrey (México), participated in the study. Correlation analyses, differences by gender, and multivariate analyses of variance, controlling for age, were performed. We found that in the maintenance stage, there is higher frequency of physical activity more healthy weight control behaviors, as well as higher autonomous motivation. In the contemplation stage, there is less physical activity, a higher frequency of unhealthy weight control behaviors, higher controlled motivation, and amotivation. Relationships were found between the healthy behaviors studied and the interaction dynamics observed across the stages of change, highlighting the key role of the contemplation and maintenance stages in weight control change. Physical activity as a targeted intervention objective could be a gateway to healthier weight control behavior, as well as higher autonomous motivation.
Substance and Behavioral Addictions, and Their Consequences among Vulnerable Populations
Authors: Sussman Steve , Sinclair Deborah Louise
Published: May 2022

“Vulnerable populations” in health behavior research, practice and policy is generally used to refer to groups that, due to their life circumstances, may require extra consideration, reasonable accommodation, and legitimized protection [...]
Factors Influencing Residents’ Behavior in Internet Recycling: From the Perspective of the Adoption of New Technology
Authors: Liu Tingting , Zheng Zichen , Wen Zhichao , Wu Shangyun , Liu Yaru , Cao Jing , Weng Zhixiong
Published: May 2022

Achieving carbon neutrality has become a major national strategy for sustainability, and the recycling of recyclable resources is an important direction toward doing so. Due to the huge amounts of recyclable resources generated every year and the low recycling rate, a new Internet recycling model with great potential to increase the recycling rate has developed rapidly in China. However, low participation from residents hinders the sustainable development of Internet recycling. Through this study, we aim to uncover potential avenues for improving Internet recycling behavior. The factors influencing Internet recycling from the perspective of new technologies have scarcely been investigated. Therefore, this study used the Unified Theory of Acceptance and Use of Technology theoretical framework to explore the factors influencing residents’ intentions and behavior toward Internet recycling. A questionnaire survey was conducted with 500 residents of Beijing, China, and empirical analysis was conducted using the structural equation model. The results indicated that social influence and performance expectancy significantly influence residents’ intentions to participate in Internet recycling, whereas effort expectancy and perceived risk do not. Facilitating conditions and behavioral intentions were identified as influential factors for use behavior. Relevant recommendations for promoting residents’ Internet recycling behavior were proposed.
Kitchen Diet vs. Industrial Diets—Impact on Intestinal Barrier Parameters among Stroke Patients
Authors: Czerwińska-Rogowska Maja , Skonieczna-Żydecka Karolina , Kaseja Krzysztof , Jakubczyk Karolina , Palma Joanna , Bott-Olejnik Marta , Brzozowski Sławomir , Stachowska Ewa
Published: May 2022

Background and aims: Strokes are the second highest cause of death in the world and the most common cause of permanent disability in adults. Intestinal barrier permeability thus contributes to diminished homeostasis within the body, which further affects the healing process and convalescence. Each stroke patient should be administered with ingredients that support the intestinal barrier (e.g., protein and fiber). The aim of this study was to compare the effect of various types of diet (enteral with or without fiber vs. a mixed kitchen diet) on the metabolic activity of intestinal microbiota, namely short chain fatty acids, and gut barrier integrity parameters (zonulin and calprotectin. Methods: Patients (n = 59), after suffering an ischemic stroke, were randomly allocated to three groups receiving: the kitchen diet (n = 32; 1.2 g fiber in 100 mL); Nutrison Energy® (n = 14; 0.02 g fiber in 100 mL); and Nutrison Diason Energy HP® (n = 13; 1.8 g fiber in 100 mL). The patients underwent anthropometric measurements and blood samples (for prealbumin measurements), and stool samples (for zonulin and calprotectin determinations) were taken twice, on admission and a week later. Results: Industrial diets enriched with fiber maintained nutritional status and had a beneficial effect on intestinal barrier permeability parameters. Patients fed with kitchen diets demonstrated a decreased number of lymphocytes, hemoglobin, erythrocytes, and increased serum concentration of C-reactive protein, but improved gut barrier markers. Proton pump inhibitors were shown to increase the inflammatory process in gut. Conclusions: Stroke patients should be administered with industrial diets enriched with fiber to improve gut barrier integrity and nutritional parameters.
Efficacy of Clinical Guidelines in Identifying All Japanese Patients with Hereditary Breast and Ovarian Cancer
Authors: Haneda Eri , Sato Ann , Suganuma Nobuyasu , Sebata Yoshiko , Okamoto Saki , Toda Soji , Kohagura Kaori , Matsubara Yuka , Sugawara Yuko , Yamanaka Takashi , Yamashita Toshinari , Shimizu Satoru , Narimatsu Hiroto
Published: May 2022

Clinical screening using the National Comprehensive Cancer Network (NCCN) testing criteria may fail to identify all patients with hereditary breast and ovarian cancers. Thus, this study aimed to evaluate the strategy of expanding target patients for genetic testing among Japanese patients. We reviewed the medical records of 91 breast cancer patients who underwent genetic testing. Among 91 patients, eight were diagnosed with pathogenic or likely pathogenic variants: BRCA1 (n = 4) and BRCA2 (n = 4). Among 50 patients meeting the testing criteria of the guidelines, 6 (12%) were diagnosed with pathogenic or likely pathogenic variants. The sensitivity and specificity of screening using the testing criteria were 75% and 47%, respectively. Expanding the NCCN criteria to include all women diagnosed with breast cancer aged ≤65 years achieved 88% sensitivity but 8% specificity. The expansion of the NCCN criteria could benefit Japanese patients; however, larger studies are necessary to change clinical practice.
The Effect of Interventions Led by Community Pharmacists in Primary Care for Adults with Type 2 Diabetes Mellitus on Therapeutic Adherence and HbA1c Levels: A Systematic Review
Authors: Al Assaf Sarah , Zelko Romana , Hanko Balazs
Published: May 2022

Type 2 diabetes mellitus has been assessed as a widespread disease globally. Unfortunately, this illness can occasionally go undetected and without symptoms until it reaches the emergency condition, and this can be notably true in patients who do not receive routine medical care. Pharmacists are the foremost accessible health care providers. They can help patients select the most appropriate hypoglycemic management strategy through their experiences. This review aimed to provide an overview of the literature published on community pharmacists’ interventions that are currently used and their usefulness in improving patient adherence and glycosylated hemoglobin (HbA1c) levels. Relevant studies were retrieved through a comprehensive search of three databases, PubMed/Medline, Web of Science, and CINAHL (2010 to 2020). In total, 8362 publications were identified. The final protocol was based on the “Preferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA)”. After applying inclusion and exclusion measures, 21 articles were deemed relevant. In pharmacists’ interventions in diabetes care, patient education and counseling were the most common intervention methods. Essentially, this systematic review provides evidence and identifies the key features that may predict success in enhancing clinical outcomes and patient adherence to treatment. Based on our findings, we suggest further investigations of the root causes of non-adherence problems.
Children’s Lived Experiences in Poverty in Hong Kong as a High-Income Asian Society
Authors: Cho Esther Yin-Nei , Wong Victor C. W.
Published: May 2022

Child poverty situated in different socioeconomic and environmental contexts has long been a central concern for practitioners, researchers, and policy makers. However, concerned research studies are predominantly adult-centric, confined to specific areas, or seldom found in Asian developed economies. Against the backdrop of this research gap, this study examines children’s experiences of poverty in relation to economic and material aspects, social relationships and participation, and psychological and emotional wellbeing, and their ways of coping with the effects of poverty. Using a purposive sampling method, a total of 40 children participants aged 8–14 living in or near poverty were recruited for an individual interview. The study showed that children experienced a range of deprivations in relation to falling short of the resources, opportunities, and activities that are commanded by average young persons. Limited living space also stands out as a more severe problem that is difficult to cope with. The various coping strategies include small spending savvy tactics, parental buffering, compensation, and mental coping. Proximity to schools and NGOs can help children in poverty to cope with problems caused by deprivations in different aspects. Implications for research studies and practice for working with children in or near poverty are discussed accordingly.
Application of Big Data Technology in Ship-to-Shore Quay Cranes at Smart Port
Authors: Li Yibo , Li Shuaihang , Zhang Qing , Xiao Binglin , Sun Yuantao
Published: May 2022

As more and more container terminals are becoming intelligent, different kinds of sensors are widely installed at different locations of the cranes and collect a large amount of data. In order to effectively utilize and manage these huge amounts of actual working data of different sensors and grasp the status of the terminal, this article proposes a data processing framework that integrates the crane load, energy consumption, crane trolley speed and crane gearbox vibration signals of the container terminal. Firstly, the load spectrum of the crane load is calculated by the non-parametric density estimation method in probabilistic statistics and the energy consumption curves are obtained. Secondly, the driving cycle of the crane trolley speed are constructed by drawing on the method in the transportation field. Finally, the vibration signals of the crane gearbox are used for anomaly detection by unsupervised methods; at the same time, clustering results can also be used as the basis for extracting typical vibration signals and removing redundant data.
Comparison of Hepatic Tissue Characterization between T1-Mapping and Non-Contrast Computed Tomography
Authors: Bardach Constanze , Morski Leonie , Mascherbauer Katharina , Donà Carolina , Koschutnik Matthias , Halavina Kseniya , Nitsche Christian , Beitzke Dietrich , Loewe Christian , Waldmann Elisabeth , Trauner Michael , Mascherbauer Julia , Hengstenberg Christian , Kammerlander Andreas
Published: May 2022

Background: Non-contrast computed tomography (CT) is frequently used to assess non-alcoholic/metabolic fatty liver disease (NAFLD/MAFLD), which is associated with cardiovascular risk. Although liver biopsy is considered the gold standard for diagnosis, standardized scores and non-contrast computed tomography (CT) are used instead. On standard cardiac T1-maps on cardiovascular imaging (CMR) exams for myocardial tissue characterization hepatic tissue is also visible. We hypothesized that there is a significant correlation between hepatic tissue T1-times on CMR and Hounsfield units (HU) on non-contrast CT. Methods: We retrospectively identified patients undergoing a non-contrast CT including the abdomen, a CMR including T1-mapping, and laboratory assessment within 30 days. Patients with storage diseases were excluded. Results: We identified 271 patients (62 ± 15 y/o, 49% female) undergoing non-contrast CT and CMR T1-mapping within 30 days. Mean hepatic HU values were 54 ± 11 on CT and native T1-times were 598 ± 102 ms on CMR and there was a weak, but significant, correlation between these parameters (r = −0.136, p = 0.025). On age and sex adjusted regression analysis, lower liver HU values indicated a dismal cardiometabolic risk profile, including higher HbA1C (p = 0.005) and higher body mass index (p < 0.001). In contrast, native hepatic T1-times yielded a more pronounced cardiac risk profile, including impaired systolic function (p = 0.045) and higher NT-proBNP values (N-Terminal Brain Natriuretic Peptide) (p = 0.004). Conclusions: Hepatic T1-times are easy to assess on standard T1-maps on CMR but only weakly correlated with hepatic HU values on CT and clinical NAFLD/MAFLD scores. Liver T1-times, however, are linked to impaired systolic function and higher natriuretic peptide levels. The prognostic value and clinical usefulness of hepatic T1-times in CMR cohorts warrants further research.
Sex-Specific Difference in the Effect of Altitude on Sleep and Nocturnal Breathing in Young Healthy Volunteers
Authors: Li Taomei , Tan Lu , Furian Michael , Zhang Yanyan , Luo Lian , Lei Fei , Xue Xiaofang , He Jiaming , Tang Xiangdong
Published: May 2022

Importance: To date, there is no established evidence of sex-specific differences in altitude-induced sleep-disordered breathing (SDB) during polysomnography-confirmed sleep. Objective: The aim of this study was to investigate whether differences in sex play a pivotal role in incidences of SDB and acute mountain sickness (AMS) when staying overnight at high altitude. Design: This was a prospective cohort study. Setting: Participants underwent overnight polysomnography (PSG) and clinical assessment in a sleep laboratory at 500 m and two consecutive days at 3270 m. Participants: The participants comprised 28 (18 women) healthy, young, low-altitude residents with a median (interquartile range) age of 26.0 (25.0, 28.0) years. Exposures: Altitude exposure. Main outcomes and Measures: The primary outcome was altitude-induced change in the PSG-confirmed apnea–hypopnea index (AHI) at 3270 m compared to 500 m between men and women. Secondary outcomes included sex differences in other parameters related to SDB, sleep structure, AMS, psychomotor vigilance test reaction time and parameters from arterial and venous blood analyses. Results: The median (interquartile range) AHIs at 500 m and 3270 m on night 1 and on night 2 were 6.5/h (3.6, 9.1), 23.7/h (16.2, 42.5) and 15.2/h (11.8, 20.9) in men, respectively, and 2.2/h (1.0, 5.5), 8.0/h (5.3, 17.0) and 7.1/h (4.9, 11.5) in women, respectively (p < 0.05 nights 1 and 2 at 3270 m vs. 500 m in men and women). The median difference (95% CI) of altitude-induced change in AHI (3270 m night 1 compared to 500 m) between men and women was 11.2/h (1.9 to 19.6) (p < 0.05). Over the time course of 2 days at 3270 m, 9 out of 18 (50%) women and 1 out of 10 (10%) men developed AMS (p < 0.05 women versus men). Conclusions and Relevance: This prospective cohort study showed that men were more susceptible to altitude-induced SDB but that they had a lower AMS incidence when staying for 2 days at 3270 m than women. These findings indicate that sex-related prevention and intervention strategies against SDB and AMS are highly warranted. Trial Registration: This trial was registered at the Chinese Clinical Trial Registry; No. ChiCTR1800020155.
Woven EndoBridge in Wide-Neck Bifurcation Aneurysms: Digital Subtraction Angiography at 3-Year Follow-Up
Authors: Stanca Carmelo , Carriero Serena , Negroni Davide , Spinetta Marco , Coda Carolina , Biondetti Pierpaolo , Guzzardi Giuseppe
Published: May 2022

Introduction: The Woven EndoBridge (WEB) device is a self-expanding intrasaccular braided-wire device for the treatment of wide-neck bifurcation aneurysms (WNBAs). Even though this device has an excellent safety profile and a low risk of rebleeding, little is known about its long-term effects. Material and Methods: All patients treated with WEB due to ruptured WNBAs were subjected to follow-up digital subtraction angiography (DSA) at 2 and 3 years after device deployment. The degree of residual neck was assessed through BOSS, Lubicz, and WEBCAST scales. Data on modified Rankin scale (mRS), bleeding events, and ischemic events occurring during this time period were collected as well. Lastly, overall and procedure-related mortality rates were calculated. Results: A total of 21 patients were treated between 1 January 2016, and 31 December 2018. DSA demonstrated a patency grade of 57.1% and 61.1% at 2 and 3 years, respectively. The overall 2-year mortality rate due to causes unrelated to the aneurysm was 14.3%. None of the patients were retreated between the 2- and the 3-year follow-up. No rebleeding or stroke events occurred during the follow-up. Conclusions: WEB-treated ruptured aneurysms showed an excellent degree of stability over time. The overall mortality rate—unrelated to the procedure–observed in our sample was higher than what reported in the literature, a possible bias associated with the COVID-19 pandemic.
Correction: Collins et al. Altimeter Observations of Tropical Cyclone-Generated Sea States: Spatial Analysis and Operational Hindcast Evaluation. J. Mar. Sci. Eng. 2021, 9, 216
Authors: Collins Clarence , Hesser Tyler , Rogowski Peter , Merrifield Sophia
Published: May 2022

Soon after the publication of the original manuscript [...]
Organic Carbon Oxidation in the Sediment of the Ulleung Basin in the East Sea
Authors: Lee Jae Seong , Kim Sung-Han , Baek Ju-Wook , Kim Kyung-Tae , Kim Dongseon , Kim Young-il , Lee Won-Chan , An Sung-Uk , Kim Chang Hwan , Park Chan Hong , Hong Sokjin
Published: May 2022

We characterized the biogeochemical organic carbon (Corg) cycles in the surface sediment layer of the Ulleung Basin (UB) of the East Sea. The total oxygen uptake (TOU) rate and the diffusive oxygen uptake (DOU) rate of the sediment were measured using an autonomous in situ benthic lander equipped with a benthic chamber (KIOST BelcII) and a microprofiler (KIOST BelpII). The TOU rate was in the range of 1.51 to 1.93 mmol O2 m−2 d−1, about double the DOU rate. The high TOU/DOU ratio implies that the benthic biological activity in the upper sediment layer is one of the important factors controlling benthic remineralization. The in situ oxygen exposure time was about 20 days, which is comparable to the values of other continental margin sediments. The sedimentary Corg oxidation rates ranged from 6.4 to 6.5 g C m−2 yr−1, which accounted for ~2% of the primary production in UB. The Corg burial fluxes ranged from 3.14 ± 0.12 to 3.48 ± 0.60 g C m−2 yr−1, corresponding to more than 30% of the deposited Corg buried into the inactive sediment deep layer.
Personalized Approaches to Stroke: One Step Forward for Functional Recovery of Stroke Patients
Authors: Chang Won Hyuk
Published: May 2022

Recent advances in diagnoses, management, and rehabilitation have had a significant impact to reduce mortality and functional recovery in stroke patients. In spite of these medical advances, many stroke survivors still suffer from significant disabilities. Stroke is a complex disease caused by a combination of multiple risk factors. Therefore, personalized medicine is more important than any other field to overcome the limitations of current stroke management and rehabilitation. It is necessary to apply accurate evaluation for functions and a personalized approach in consideration of various characteristics of each stroke patient to improve function. The objective of this Special Issue is to inform the recent scientific knowledge, current limitations, and challenges for an individually tailored strategy in the areas of diagnosis, treatment, and rehabilitation of stroke. A multidisciplinary approach and research will be strongly encouraged for personalized medicine in the field of stroke treatment and rehabilitation.
Exposure to Proton Pump Inhibitors and the Risk of Incident Asthma in Patients with Coronary Artery Diseases: A Population-Based Cohort Study
Authors: Lin Tsung-Kun , Tsai Chin-Feng , Huang Jing-Yang , Pan Lung-Fa , Jong Gwo-Ping
Published: May 2022

We aimed to determine the association between proton pump inhibitor (PPI) use and incident asthma in patients with coronary artery disease (CAD). This nationwide cohort study collected claims data from the Taiwanese Bureau of National Health Insurance from 2004 to 2013. The primary outcome, i.e., the risk of incident asthma, was assessed by estimating hazard ratios (HRs) and 95% confidence intervals (CIs). The adjusted HR of asthma development was estimated using the Cox regression model. Sensitivity and subgroup analyses were also conducted. A total of 8894 PPI users and 12,684 H2-receptor antagonist (H2RA) users were included in patients with CAD. Compared with H2RA use, an increased risk of incident asthma was found between PPI use and the risk of incident asthma in patients with CAD after adjusting for sex, age, urbanization, and low income (HR: 1.41; 95% CI: 1.04–1.89). The sensitivity analysis results were consistent with the main analysis results. However, the subgroup analysis revealed no association of incident asthma in patients with diabetes mellitus, hyperlipidemia, stroke, allergic rhinitis, pneumonia, cancer, or depression in the PPI group compared with those in the H2RA group. In conclusion, PPI use increased the risk of asthma development in patients with CAD.
“Do I Have to Sign My Real Name?” Ethical and Methodological Challenges in Multilingual Research with Adult SLIFE Learning French as a Second Language
Authors: H. Michaud Alexandra , Fortier Véronique , Amireault Valérie
Published: May 2022

In 2017, Quebec’s Auditor General reported several major issues regarding government-funded French as a second language (FSL) courses, especially those intended for adult students with limited or interrupted formal education (SLIFE). To this day, no official framework or program exists for this specific population, a situation that the government of Quebec wishes to resolve. Our research team was thereby mandated by the Ministry of Immigration to conduct a large-scale multilingual study with the objective of gaining a better understanding of the realities and needs of the various stakeholders involved in low-literate FSL classes. We met 42 teachers, 24 French learning center directors, and 10 pedagogical advisors in individual interviews; we also led 107 group interviews with SLIFE in 26 languages, allowing us to meet 464 adult SLIFE enrolled in low-literate FSL classes from 11 regions of the province of Quebec, most of them being refugees. This article reports on the decision-making process in which we engaged to overcome the ethical and methodological challenges we faced at various stages of the data collection with SLIFE participants: recruitment, informed consent, confidentiality, interview protocol design, instrument piloting, data collection, and data translation and transcription. To make informed decisions, we had to turn to literature outside SLA (i.e., refugee research and translation/interpreting literature) for guidance. In this article we discuss the limitations and contributions of our research to guide researchers who will conduct studies with similar non-academic samples/populations.
B, O and N Codoped Biomass-Derived Hierarchical Porous Carbon for High-Performance Electrochemical Energy Storage
Authors: Kong Shuying , Xiang Xinzhu , Jin Binbin , Guo Xiaogang , Wang Huijun , Zhang Guoqing , Huang Huisheng , Cheng Kui
Published: May 2022

High specific surface area, reasonable pore structure and heteroatom doping are beneficial to enhance charge storage, which all depend on the selection of precursors, activators and reasonable preparation methods. Here, B, O and N codoped biomass-derived hierarchical porous carbon was synthesized by using KCl/ZnCl2 as a combined activator and porogen and H3BO3 as both boron source and porogen. Moreover, the cheap, environmentally friendly and heteroatom-rich laver was used as a precursor, and impregnation and freeze-drying methods were used to make the biological cells of laver have sufficient contact with the activator so that the layer was deeply activated. The as-prepared carbon materials exhibit high surface area (1514.3 m2 g−1), three-dimensional (3D) interconnected hierarchical porous structure and abundant heteroatom doping. The synergistic effects of these properties promote the obtained carbon materials with excellent specific capacitance (382.5 F g−1 at 1 A g−1). The symmetric supercapacitor exhibits a maximum energy density of 29.2 W h kg−1 at a power density of 250 W kg−1 in 1 M Na2SO4, and the maximum energy density can reach to 51.3 W h kg−1 at a power density of 250 W kg−1 in 1 M BMIMBF4/AN. Moreover, the as-prepared carbon materials as anode for lithium-ion batteries possess high reversible capacity of 1497 mA h g−1 at 1 A g−1 and outstanding cycling stability (no decay after 2000 cycles).
Molecular Dynamics Analysis of Graphene Nanoelectromechanical Resonators Based on Vacancy Defects
Authors: Li Wenhua , Tian Wenchao
Published: May 2022

Due to the limitation of graphene processing technology, the prepared graphene inevitably contains various defects. The defects will have a particular influence on the macroscopic characteristics of the graphene. In this paper, the defect-based graphene nanoresonators are studied. In this study, the resonant properties of graphene were investigated via molecular dynamic simulations. The effect of vacancy defects and hole defects at different positions, numbers, and concentrations on the resonance frequency of graphene nanoribbons was studied. The results indicated that single monatomic vacancy has no effect on graphene resonant frequency, and the concentration of the resonant frequency of graphene decreases almost linearly with the increase of single-atom vacancy concentration. When the vacancy concentration is 5%, the resonance frequency is reduced by 12.77% compared to the perfect graphene. Holes on the graphene cause the resonance frequency to decrease. As the circular hole defect is closer to the center of the graphene nanoribbon, not only does its resonant frequency increase, but the tuning range is also expanded accordingly. Under the external force of 10.715 nN, the resonant frequency of graphene reaches 429.57 GHz when the circular hole is located at the center of the graphene nanoribbon, which is 40 GHz lower than that of single vacancy defect graphene. When the circular hole is close to the fixed end of graphene, the resonant frequency is 379.62 GHz, which is 90 GHz lower than that of single vacancy graphene. When the hole defect is at the center of nanoribbon, the frequency tunable range of graphene reaches 120 GHz. The tunable frequency range of graphene is 100.12 GHz when the hole defect is near the fixed ends of the graphene nanoribbon. This work is of great significance for design and performance optimization of graphene-based nanoelectro-mechanical system (NEMS) resonators.
Green Synthesis of Silver Nanoparticles Using Allium cepa var. Aggregatum Natural Extract: Antibacterial and Cytotoxic Properties
Authors: Shanmugam Jayashree , Dhayalan Manikandan , Savaas Umar Mohammed Riyaz , Gopal Mayakkannan , Ali Khan Moonis , Simal-Gandara Jesus , Cid-Samamed Antonio
Published: May 2022

The chemical content of plant excerpts can be efficiently employed to reduce the metal ions to nanoparticles in the one-pot green production method. Here, green production of silver nanoparticles (AC-AgNPs) is performed by means of Allium cepa var. Aggregatum (shallot) extract as a stabilizer and reducer. The shape, size, and morphology of resultant AC-AgNPs are examined by optical spectroscopy analysis such as UV for nucleation and coalescence processes of the AC-AgNPs. Through FTIR functional group is determined and through DLS size is defined, it was confirmed that metallic AgNPs were successfully synthesized through the green synthesis route, and these results agreed well with the results obtained in the XRD pattern along with TEM spectroscopy, where the TEM images confirm the formation of sphere-like nanostructures along with SAED analysis. The chemical characterization is performed with XPS; the obtained molecular species in the materials are determined from the energy profile. Antioxidant activity of AC-AgNPs versus DPPH substrate is carried out. Antibacterial activity is well established against Gram-negative and Gram-positive organisms. Cell viability is accomplished, followed by an MTT assay, and a cytotoxicity assay of AC-AgNPs on MCF—7 cell lines is also carried out. Highlights: (1). This study highlights the eco-friendly synthesis of silver nanoparticles from Allium cepa var. Aggregatum Natural Extract. (2). The synthesized AC-AgNPs were characterized by UV-VIS, FT-IR, XRD, TEM, and XPS. (3). The synthesized nanoparticles were well dispersed in nature and the size range of 35 ± 8 nm. (4). The anti-candidal activity of biosynthesized silver nanoparticles was evaluated against the following Gram-Negative organisms: Escherichia coli (E. coli), and the following Gram-positive organisms: Staphylococcus aureus strains. The biosynthesized AC-AgNPs showed enhanced antiseptic features anti both Gram-positive and negative organisms. (5). Besides, the in vitro cytotoxic outcomes of AC-AgNPs were assessed versus MCF-7 cancerous cells, and the reduction in the feasibility of cancer cells was established via MTT assay, which suggests potential biomedical applications.
Ultralight, Mechanically Enhanced, and Thermally Improved Graphene-Cellulose-Polyethyleneimine Aerogels for the Adsorption of Anionic and Cationic Dyes
Authors: Wang Xiuya , Xie Pengbo , He Lan , Liang Yuwei , Zhang Liang , Miao Yuanyuan , Liu Zhenbo
Published: May 2022

Graphene-cellulose-polyethyleneimine aerogels (GA-MCC-PEI) were prepared using a simple, environmentally friendly method to remove anionic and cationic dyes in water. Graphene-cellulose hydrogels were prepared using a hydrothermal method and then immersed in a polyethyleneimine aqueous solution for 48 h to obtain graphene-cellulose-polyethyleneimine hydrogels, which were then freeze-dried. The light and porous composite aerogels had a good compression resistance, and the maximum allowable pressure of the graphene-cellulose-polyethyleneimine aerogel with a cellulose content of 43% was 21.76 kPa, which was 827 times its weight. Adsorption of the anionic dye amaranth and the cationic dye methylene blue by the graphene-cellulose-polyethyleneimine aerogel was satisfactorily modeled using the Langmuir isothermal equation, indicating monolayer adsorption. When the cellulose content was 39%, the equilibrium adsorption capacities of the composite aerogel for amaranth and methylene blue were 369.37 mg/g and 237.33 mg/g, respectively. This graphene-cellulose-polyethyleneimine aerogel can be used to remove dye pollutants in water to maintain ecological balance, thus broadening the application space of aerogel materials, that is, as adsorbents in different environments.
Tunable Dual-Broadband Terahertz Absorber with Vanadium Dioxide Metamaterial
Authors: Feng Hengli , Zhang Zuoxin , Zhang Jingyu , Fang Dongchao , Wang Jincheng , Liu Chang , Wu Tong , Wang Guan , Wang Lehui , Ran Lingling , Gao Yang
Published: May 2022

A dual broadband terahertz bifunction absorber that can be actively tuned is proposed. The optical properties of the absorber were simulated and numerically calculated using the finite-difference time-domain (FDTD) method. The results show that when the conductivity of vanadium dioxide is less than σ0=8.5×103 S/m, the absorptance can be continuously adjusted between 2% and 100%. At vanadium dioxide conductivity greater than σ0=8.5×103 S/m, the absorption bandwidth of the absorber can be switched from 3.4 THz and 3.06 THz to 2.83 THz and none, respectively, and the absorptance remains above 90%. This achieves perfect modulation of the absorptance and absorption bandwidth. The physical mechanism of dual-broadband absorptions and perfect absorption is elucidated by impedance matching theory and electric field distribution. In addition, it also has the advantage of being polarization insensitive and maintaining stable absorption at wide angles of oblique incidence. The absorber may have applications in emerging fields such as modulators, stealth and light-guided optical switches.
Early Days of SACLA XFEL
Authors: Ishikawa Tetsuya
Published: May 2022

The SACLA (SPring-8 Angstrom compact laser) was designed to significantly downsize the SASE (self-amplified spontaneous emission) type XFEL (X-ray free-electron laser), in order to generate coherent light in the wavelength region of 0.1 nm by adopting an in-vacuum undulator that can shorten the magnetic field period length. In addition, a SASE XFEL facility with a total length of 700 m has become a reality by using a C-band RF accelerating tube that enables a high acceleration gradient. Although progress was initially slow, the small-scale, low-cost SACLA was smoothly constructed, and it became the second light source to lase in the 0.1 nm wavelength region, following the LCLS (linac coherent light source) in the United States. In this paper, we look back on the history leading up to SACLA. and describe the SCSS (SPring-8 compact SASE source) project as a preparatory stage and a part of the construction/commissioning of SACLA. Since March 2012, SACLA has been operating as a shared user facility. Just a few of the upgrade activities of the facility and advanced research conducted are introduced. Finally, we will discuss the future development of the SPring-8 site, which has co-located the third-generation synchrotron radiation facility SPring-8 and the X-ray free-electron laser facility SACLA.
Health Beneficial Phytochemicals in Dioscorea caucasica Lipsky Leaves and Tubers and Their Inhibitory Effects on Physiologically Important Enzymes
Authors: Adomėnienė Aušra , Pukalskas Audrius , Ragažinskienė Ona , Venskutonis Petras Rimantas
Published: May 2022

Dioscorea caucasica Lipsky is a tertiary relict endemic plant naturally growing in the western part of the trans-Caucasus regions; it has adapted and successfully grows in the temperate region of the Baltic countries. Information about its phytochemical composition and bioactivities is rather scarce. This study reports the results of the identification of 41 compounds in D. caucasica leaf and tuber hydroethanolic extracts using UPLC-QTOF/MS. Organic acids were found in both extracts; hydroxycinnamates and flavonoids were the main phytochemicals in the leaves, while steroidal glycosides, fatty acids (mainly hydroxylated) and carbohydrates were found in the tubers. Leaf extracts inhibited enzymes in a dose-dependent manner and were remarkably stronger inhibitors of physiologically important enzymes, namely α-amylase (48.6% at 480 µg/mL), α-glucosidase (IC50 = 41.99 and 47.95 µg/mL with and without 0.1 M Na2CO3), acetylcholinesterase (45.85% at 100 µg/mL) and angiotensin-converting enzyme (IC50 = 829.7 µg/mL), most likely due to the presence of some quantified polyphenolic antioxidants. The mode of inhibition of α-glucosidase and acetylcholinesterase was assessed via kinetic studies based on Lineweaver–Burk inhibition plots. Leaf and tuber extracts acted as mixed-type and competitive inhibitors of α-glucosidase, respectively; the leaf extract demonstrated an uncompetitive inhibition mode of acetylcholinesterase. It is expected that this new knowledge of D. caucasica will serve for its valorization in developing new health beneficial ingredients for functional foods and nutraceuticals.
Rheological Properties of Cholesteric Liquid Crystal with Visible Reflection from an Etherified Hydroxypropyl Cellulose Derivative
Authors: Matsumoto Kazuma , Ogiwara Yuki , Iwata Naoto , Furumi Seiichi
Published: May 2022

Optical properties of hydroxypropyl cellulose (HPC) derivatives have been widely investigated for their ability to exhibit cholesteric liquid crystal (CLC) phase. However, there are only a limited number of studies on their rheological properties even though they are quite important for the applications of such HPC derivatives to the versatile CLC photonic devices. In this article, we report on the optical and rheological properties of an HPC derivative possessing pentyl ether groups in the side-chains. The etherified HPC derivative exhibited thermotropic CLC phase with light reflection in the temperature range between 25 °C and 120 °C. After the HPC derivative was heated once at isotropic phase, followed by being cooled to the CLC phase, the reflection peak could not be observed, even at the CLC phase. At this stage, the HPC derivative exhibited solid-like rheological responses compared to that of sheared at a constant shear rate of 1.0 s−1. Such differences in the optical and rheological properties of the HPC derivative can be ascribed to the difference in CLC orientation state. From the rheological results, the etherified HPC derivative showed liquid-like behavior rather than the esterified HPC derivatives. This evidence provides a promising clue for fabricating high-quality CLC devices by the facile CLC orientation.
New Antioxidant Active Packaging Films Based on Yeast Cell Wall and Naphtho-γ-Pyrone Extract
Authors: Rezzani Guillermo , Choque Elodie , Salvay Andrés , Mathieu Florence , Peltzer Mercedes
Published: May 2022

The main objective of this work is the development of new active films based on yeast cell wall obtained by high-pressure homogenization (YCW-H) supplemented with naphtho-γ-pyrone (CL-NGP) extract, which is a bioactive compound produced by Aspergillus tubingensis G131 with great antioxidant potential. A complete characterization of the functional properties of the bioactive films, such as their structural, colour, thermal, mechanical, hydration and water vapour transport, was carried out to evaluate the influence of the addition of the antioxidant compounds. Likewise, the antioxidant capacity of the developed materials and the specific migration of NGPs in food simulants were evaluated. The results showed that CL-NGP extract possessed an important antioxidant activity, which was maintained after incorporation in YCW-H films. The addition of 2 and 5% CL-NGPs decreased the hydration of films and consequently improved the water vapour barrier properties. It was observed that CL-NGPs migrate in fatty food simulants and retain their antioxidant capacity in the simulant. The results obtained in this work showed that bioactive films based on yeast cell walls with the addition of CL-NGPs have the potential to be used as packaging material in systems of interest in the food industry.
Effects of Wheat Bran Micronization on the Quality of Reconstituted Whole-Wheat Flour and Its Cooked Noodles
Authors: Lai Sitong , Liu Jinlong , Zhang Yanqing , Wang Yuanmeng , Li Guang , Cui Qingliang
Published: May 2022

The particle size of wheat bran plays an important role in the quality of reconstituted whole-wheat flour and its products. The effects of wheat bran particle size on the quality of reconstituted whole-wheat flour and its cooked noodles were analyzed; the mean particle size (D50) of wheat bran ranged from 26.05 to 46.08 μm. Results show that the decreases in D50 of wheat bran induced the changes in the quality of whole-wheat flour and its noodles. Specifically, the damaged starch content, water absorption, and the solvent retention capacity of sodium carbonate and sucrose of whole-wheat flour increased at various degrees, while pasting viscosity decreased, and the gluten index and SDS-sedimentation volume increased first and then decreased. The cooking yield, cooking loss, and break rate of fresh noodles decreased first and reached a trough at D50 of 26.05 μm, and then increased. The adhesiveness of cooked noodles increased, the score of smoothness, taste, appearance, and color increased to a stable value, but the hardness, springiness, cohesiveness, resilience, firmness score, and elasticity score increased first and then decreased. These turning points of changing trends of indexes mostly occurred when the D50 of wheat bran was 26.51 μm. In conclusion, whole-wheat noodles with wheat bran of D50 of 26.51 μm addition exhibit better cooking, textural, and sensory properties than those with smaller or larger wheat bran. Excessive crushing of wheat bran not only costs highly in terms of energy, but also has a negative impact on the quality of the noodles.