bluuebunny/crossref_metadata_2025 huggingface dataset:
Perbandingan Model Pembelajaran Inquiry dan Model Pembelajaran Relating, Experiencing, Applying, Cooperating, and Transfering Terhadap Pemahaman Matematis
Authors: Aditya Reinaldo  , Thamrin Tayeb  , Fitriani Nur  , Suharti  , Munawarah
Published: January 2022

Abstrak

Penelitian ini bertujuan untuk mengetahui perbedaan antara pemahaman matematis siswa yang diajar menggunakan model pembelajaran Inquiry dan model pembelajaran REACT. Jenis penelitian ini yaitu eksperimen semu (Quasi Experimental) dengan desain Nonequivalent Control Group Design yang dilaksanakan di SMP Negeri 5 Pitumpanua Kabupaten Wajo. Teknik dan instrumen pengumpulan data menggunakan tes dan dianalisis menggunakan analisis statistik deskriptif. Berdasarkan hasil analisis data diperoleh hasil pretest dan posttest siswa yang diajar dengan model pembelajaran Inquiry diperoleh nilai rata-rata pretest sebesar 47,50 pada kategori sedang dan nilai rata-rata posttest  sebesar 65,00 pada kategori tinggi. Hasil pretest dan posttest siswa yang diajar dengan model pembelajaran REACT diperoleh nilai rata-rata pretest sebesar 51,00 pada kategori sedang dan nilai rata-rata posttest  sebesar 71,67 pada kategori tinggi. Artinya, terdapat perbedaan antara pemahaman matematis siswa yang diajar dengan menggunakan model pembelajaran Inquiry dengan pemahaman matematis siswa yang diajar dengan menggunakan model pembelajaran REACT.

Abstract

This study aims to determine the differences between students' mathematical understanding who are taught using the Inquiry learning model and the REACT learning model. This type of research is a quasi-experimental (Quasi Experimental) design with Nonequivalent Control Group Design which was implemented at SMP Negeri 5 Pitfeedua, Wajo Regency. Data collection techniques using test techniques and analyzed using descriptive statistical analysis. Based on the results of data analysis, the pretest and posttest results of students who were taught with the Inquiry learning model obtained an average pretest score of 47.50 in the medium category and an average posttest score of 65.00 in the high category. The results of the pretest and posttest students who were taught with the REACT learning model obtained an average pretest score of 51.00 in the medium category and an average posttest score of 71.67 in the high category. That is, there is a difference between the mathematical understanding of students who are taught using the Inquiry learning model and the mathematical understanding of students who are taught using the REACT learning model in grade VIII students of SMP Negeri 5 Pitfeedua, Wajo Regency.
MENINGKATKAN KEMAMPUAN PEMAHAMAN SERTA MENURUNKAN KECEMASAN MATEMATIS MELALUI STRATEGI BRAIN BASED LEARNING
Authors: Fikriyah Dea Siti , Maharani Gitta , Nugraha M. Gilang Agustian , Mubarika Melinda Putri
Published: January 2022

Tujuan dari penelitian ini adalah untuk menganalisis: (1) peningkatan kemampuan pemahaman matematis siswa yang memperoleh pembelajaran dengan strategi Brain Based Learning lebih baik daripada siswa yang memperoleh pembelajaran konvensional ditinjau dari perbedaan gender; (2) Bagaimana kecemasan matematis siswa yang memperoleh pembelajaran dengan strategi Brain Based Learning lebih baik daripada siswa yang memperoleh pembelajaran konvensional ditinjau dari perbedaan gender, (3) Apakah terdapat hubungan antara peningkatan kemampuan pemahaman matematis serta kecemasan matematis siswa ditinjau dari perbedaan gender. Metode penelitian menggunakan metode campuran (mixed methods) tipe embedded design. Populasi penelitian adalah kelas VIII SMP Pasundan 4 Bandung dengan pengambilan sampel menggunakan teknik purposive sampling. Instrumen yang digunakan adalah tes dan non tes, yaitu tes pemahaman matematis, sedangkan non tes yaitu observasi, wawancara. Hasil penelitian ini adalah: (1) Peningkatan kemampuan pemahaman matematis siswa yang memperoleh pembelajaran dengan strategi Brain Based Learning lebih baik daripada siswa yang memperoleh pembelajaran konvensional ditinjau dari perbedaan gender; (2)  Siswa yang menggunakan pembelajaran dengan strategi Brain Based Learning mempunyai tingkat kecemasan yang lebih rendah dibandingkan dengan siswa yang menggunakan pembelajaran konvensional; (3) Tidak terdapat hubungan antara kecemasan matematis siswa terhadap kemampuan pemahaman matematis siswa.
Pembelajaran Generatif dengan Pendekatan Kontekstual Berbasis Daring untuk Meningkatkan Soft Skill Siswa
Authors: Yenni Yenni
Published: January 2022

Tujuan penelitian ini adalah untuk mengetahui apakah terdapat peningkatan soft skill antara siswa yang diberi model pembelajaran generatif dengan pendekatan kontekstual, dan siswa yang diberi metode konvensional. Penelitian ini menggunakan metode Quasi eksperiment dengan jenis Nonequivalent Control Group Design. Populasi dalam penelitian ini adalah siswa kelas VIII MTs Insan Kreasi. Sampel berjumlah 65 siswa, dengan 34 siswa pada grup eksperimen dan 31 siswa pada grup kontrol. Teknik pengambilan data menggunakan acak kelas. Hasil penelitian menunjukkan bahwa terdapat perbedaan peningkatan soft skill siswa antara siswa yang menggunakan model pembelajaran generatif dengan pendekatan kontekstual, dan siswa yang menggunakan model pembelajaran konvensional. Hal tersebut berdasarkan hasil uji hipotesis dengan menggunakan uji-t diperoleh yaitu , maka terdapat perbedaan yang signifikan antara skor postest soft skill siswa grup eksperimen dan siswa grup kontrol. Hasil uji n-gain diperoleh nilai grup eksperimen > nilai grup kontrol yaitu 0.255>0.031. Artinya terdapat peningkatan soft skill siswa siswa grup eksperimen dan grup kontrol
Media Hippo Animator pada Pembelajaran Statistika dalam Upaya Meningkatkan Kemampuan Statistik dan Self-confidence Mahasiswa di Era Pandemi Covid 19
Authors: Hidayat Abas , Juliyanto Perdana Fani
Published: January 2022

Di Era Pandemi Covid 19 perubahan terjadi yaitu pada proses pembelajaran. Proses pembelajaran menjadi  tidak dibatasi oleh ruang dan waktu. Penggunaan ICT merupakan sebuah tantangan dan salah satu bentuk motivasi bagi pengajar untuk dapat berinovasi dalam gaya atau metode mengajar. Hippo Animator memiliki fleksibelitas bagi peserta didik dapat digunakan secara online dan offline. Tujuan Penelitian ini yaitu menganalsis penggunaan Hippo animator dalam upaya mengoptimalkan hasil belajar secara daring di era pandemi covic 19. Penelitian ini menggunakan pendekatan kuantitatif melalui desain kuasi-eksperimen. Subjek penelitian ini adalah 164 mahasiswa. 2 kelas adalah kelas kontrol sebanyak 82 mahasiswa.  2 kelas adalah kelas eksperimen sebanyak 82 mahasiswa. Hasil penelitian ini adalah penggunaa Hippo Animasi dapat meningkatkan kemampuan statistika dan self-confidence secara signifikan. Selain itu, dapat meningkatkan kemampuan melek statistik (statistical literacy), penalaran statistik (statistical reasoning) dan berfikir statistik (statistical thinking) secara signifikan. Penerapan Hippo Aminator pada penelitian ini memberikan warna berbeda dalam pembelajaran statistika.
Effect of Mobilization with Movement and Kinesio Taping on Knee Function and Pain in Gonarthrosis
Authors: Pažemeckas Tomas , Jurgelaitienė Giedrė , Janušonienė Laura
Published: January 2022

Background.  Gonarthrosis is one of the most common diseases of the musculoskeletal system. As the disease progresses, the patient’s social and physical activities change. Reliable and effective therapies that slow the progression of gonarthrosis have not yet been found. There is a lack of evidence that a short course of physiotherapy and exercise-based treatment is effective or of how mobilization with movement and kinesio taping affects knee function and pain. We believe that in patients with knee gonarthrosis, the positive effects of physiotherapy combined with mobilization with movement and kinesio taping on knee functions and pain will be better than in those subjects who received only traditional physiotherapy.

Aim. To determine the effect of mobilization with movement and kinesio taping on knee function parameters and pain in gonarthrosis.

Methods. The study included 22 people diagnosed with gonarthrosis. For the experimental group (n=11), physiotherapy exercise, mobilization with movement and kinesiotape were applied, whereas the control group (n=11) used only physiotherapy exercise procedures. Subjects were evaluated for pain intensity, active knee range of motion, dynamic knee joint stability and evaluation of thigh muscle strength.

Results. In the experimental group, there was a significant decrease in pain after 7 days and a significant difference with control group. Significant knee flexion results were recorded in the experimental group and significant knee extension results were recorded in the control group. In the experimental group, the dynamic knee joint stabilization significantly increased after 14 days. The strength of the thigh muscles was significantly increased in the experimental group after 14 days.

Conclusion. The effect of mobilization with movement and kinesio taping of knee function, dynamic stability pain improving functional performance of dynamic stability was more significant than an exercise program lasting two weeks in subjects with knee gonarthrosis.

Keywords: gonarthrosis, mobilization with movement, kinesio taping.
Impact of Physiotherapy with Unstable Platforms for the Prevention of Ankle Injuries and Improving Balance in Male Volleyball Players
Authors: Stankevičius Patrikas , Sipavičienė Saulė
Published: January 2022

Background. Ankle sprain is the most common sports injury. Conducting physiotherapy with unstable platforms efficiently decreases risk of ankle injuries and increases static and dynamic balance parameters.

Aim. To estimate the effect of physiotherapy on balance of volleyball players and the prevention of ankle injuries.

Methods. The study involved 10 male volleyball players, who were randomly divided into two groups: intervention (physiotherapy with unstable platforms) and control (no physiotherapy applied). Flamingo test and modified star excursion balance test were used before and after intervention. Duration of intervention – 4 weeks.

Results. After 4 weeks of physiotherapy with unstable platforms static balance increased in intervention group subjects by 66.7% (p < 0,05); however, there were no changes in the control group. Post intervention examination showed statistically significant increase in Y balance test results in different directions, while the control group did not show any change.

Conclusion. After a physiotherapy program with unstable platforms, static balance as well as dynamic balance increased. A 4-week physiotherapy program with unstable platforms significantly decreased the risk of ankle injuries in male volleyball players.

Keywords: balance, ankle injury prevention, volleyball.
Impact of Aerobic and Strength Exercises on Anthropometric Parameters in Overweight and Obese Women
Authors: Tarasenko Viktorija
Published: January 2022

Background. Overweight and obesity are becoming an increasing problem in the world. As body weight increases, so does the risk of obesity-related diseases and the economic burden. More research is needed to help improve the physical and emotional well-being of overweight people.

The aim. To evaluate the effects of aerobic and strength exercises on body weight and the anthropometric indicators of overweight and obese women.

Methods. The study included 11 women who were overweight. Their height, weight and body mass index were measured. Subjects underwent an aerobic and strength exercise program that was administered 3 times per week for 9 weeks. The duration of each session was 1 hour.

Results. Subjects lost 2.2 ± 1.4 kg after the aerobic and strength exercise program. Chest, torso, hip and thigh circumferences decreased (p <0.05).

Conclusion. An aerobic and strength exercise program lasting nine weeks can reduce body weight and body circumferences in overweight women.

Keywords: overweight, obesity, aerobic exercise, strength exercise, anthropometric indicators.
Attitude and Knowledge of Occupational Therapists and Physiotherapists Towards the Use of Research-Based Methods in Children with Cerebral Palsy
Authors: Udraitė Justina , Prasauskienė Audronė
Published: January 2022

Background. More and more alternative treatments and therapies are emerging, so it is very important for professionals not only not to get lost in their abundance, but also to choose the right methods.

Aim. To find out the attitude of occupational therapists and physiotherapists towards the evidence-based therapeutic methods in rehabilitation for children with cerebral palsy.

Methods. 73 rehabilitation specialists participated in this study, 30 of them were occupational therapists and 43 were physiotherapists and filled in the questionnaire. Respondents answered about the attitude and knowledge towards the evidence-based therapeutic methods in rehabilitation for children with cerebral palsy.

Results. 97.3% respondents answered that they use evidence-based practice methods in their work. The number of scientific articles read per month in the range from 0 to 5 articles was chosen by 71.2% respondents, from 6 and more articles – 28,8% respondents. The interface between the number of articles read per month by occupational therapists was directly and moderately strongly related to work experience (r=0.555; p=0.001). The education of occupational therapists and physiotherapists was weakly related to the frequency with which they used evidence-based methods in their work (r=0.374; p=0.042; r=0.476; p=0.001).

Conclusions. The study revealed that rehabilitation specialists apply evidence-based practice methods in their work. It can be argued that the higher the level of education a rehabilitation specialist has, the more they value evidence-based practices and apply them in their work.

Keywords: cerebral palsy, methods of children rehabilitation, evidence-based practise.
Effects of Online Facial Exercise and Self-Massage Program on Neck and Head Pain and Quality of Life
Authors: Dapkevičiūtė Medeinė , Žlibinaitė Laura
Published: January 2022

Background. Due to the COVID-19 pandemic and the lockdown, most people with headache and neck pain were unable to access health care services, so it is unclear which remote physiotherapy methods would be effective in treating neck and head pain.

Aim. To investigate the effects of virtual facial exercise and self-massage on women’s neck and head pain and functional condition.

Methods. The study included 33 women 45–59 years old, who had experienced neck and head pain in the past month and were not taking pain medications. All subjects were in the experimental group. Facial exercise and self-massage for 20 minutes daily for 20 days were performed by the subjects following virtual instructions. Before and after the intervention, the intensity of neck and head pain was assessed using numeric pain intensity scale, the quality of life was assessed using neck disability index and headache disability index.

Results. Neck pain decreased by 3.82 ± 2.63 points (p < 0.05), headache decreased by 2.82 ± 1.93 points (p < 0.05), neck functional disability decreased by 7.33 ± 4.57 points (p < 0.05), headache disability index values decreased by 15.57 ± 8.47 points (p < 0.05).

Conclusion. After the application of virtual facial exercise and self-massage, women’s neck and head pain was reduced and the quality of life improved.

Keywords: neck pain, headache, physiotherapy, massage, quality of life.
Acousmatic Orphism: Susan Howe
Authors: Donahue Joseph
Published: January 2022

In this essay Joseph Donahue uncovers the Orphic ambitions of Susan Howe's 2010 volume of poetry, That This, especially as manifested in the poet's collaboration with the composer David Grubbs in the recording of a poem from that volume, ‘Frolic Architecture’. To account for the use of free-floating syllabic sound as an intensification of the Orphic concerns of the poem in the recording, the essay turns at first to the origin of acousmatic sound and its proposed relations to ancient mystery cults: composer and sound theorist Pierre Schaeffer claimed that to hear sound without seeing its source placed the listener in a position comparable to that of an initiate in the cult of Pythagoras. Drawing on Brian Kane's 2014 study of the origins of musique concrète (which incorporates recorded sounds) in the postwar period, Sound Unseen, this piece claims the acousmatic not only for Pythagoras but for Orpheus. It is argued that an Orphic poetics rooted in the acousmatic comes to full fruition in late Howe. Howe's own evocations of Pythagoras, and her own mythologising of the acousmatic, are examined, especially in regard to her collage method which so often and so momentously conceals or removes the visual origin of sounded syllables. The collaboration with composer David Grubbs intensifies the acousmatic poetics of Howe's text, and it is suggested, is the poem's ultimate realisation.
You have an academic job offer…Now what? Negotiating advice from two perspectives
Authors: O'Brochta William , Poloni-Staudinger Lori
Published: January 2022

This chapter describes the process of receiving, negotiating, and accepting a job offer with perspectives from both a faculty member and a dean. This manuscript is part of Strategies for Navigating Graduate School and Beyond, a forthcoming volume for those interested in pursuing graduate education in political science (Fall 2022 publication).
ИСТОРИЯ ПРАВОСЛАВНОЙ КУЛЬТУРЫ РОССИИ (ПРОГРАММА ДЛЯ НЕГУМАНИТАРНОГО ВУЗА)
Authors: Кириченко Олег Викторович , Стракач Юрий Борисович , Мелехова Галина Николаевна , Моклецова Ирина Васильевна
Published: January 2022

Представленная программа создана для преподавания дисциплины «История православной культуры России» студентам негуманитарных вузов. Программа содержит остававшиеся ранее без внимания факты и концепции, имеющие своей основой православную религиозность и культуру.
ДОМ КАК СРЕДА ПРОЯВЛЕНИЯ РЕЛИГИОЗНОСТИ: СВЯТЫНИ, ОСВЯЩЕННЫЕ ПРЕДМЕТЫ И СВЯЗАННЫЕ С НИМИ ПРЕДСТАВЛЕНИЯ В СОВРЕМЕННЫХ РУССКИХ ПРАВОСЛАВНЫХ СЕМЬЯХ
Authors: Шляхтина Наталья Валерьевна
Published: January 2022

Статья обращена к редкой теме современного «религиозного быта» на начальным этапе обращения к традиции. Написана на современных этнографических материалах, собранных в экспедициях.
РЕЦЕНЗИЯ НА УЧЕБНОЕ ПОСОБИЕ ПО ИСТОРИИ И ОБЩЕСТВОЗНАНИЮ ДЛЯ СРЕДНЕЙ ШКОЛЫ "МОЗАИКА КУЛЬТУР". АВТОРЫ В. ШАПОВАЛ, И. УКОЛОВА, О. СТРЕЛОВА, С. ЯЛОВИЦЫНА, М. ЕРОХИНА, И. ХРОМОВА, А. ЦУЦИЕВ, Ю. КУШНЕРЕВА, Л. ГАТАГОВА. ПОД РЕД. А. П. ШЕВЫРЕВА, Т. Н. ЭЙДЕЛЬМАН. М., 2005
Authors: Кириченко Олег Викторович
Published: January 2022

В рецензии на учебное пособие «Мозаика культур», созданное международным коллективом авторов, отмечено, что данное пособие не может быть допущено в школу по причине крайней его тенденциозности.
ТРАДИЦИЯ С ПОЗИЦИИ ПРАВОСЛАВНОГО МИРОВОЗЗРЕНИЯ
Authors: Кириченко Олег Викторович
Published: January 2022

В статье затрагивается вопрос понимания традиции как общего для всего человечества периода истории. Таких периодов автор выделяет два: дохристианский и христианский. В их рамках можно проследить свои внутренние модели традиционности.
РЕЦЕНЗИЯ НА КНИГУ Н. К. ЧЕРНЫШЕВОЙ "ПОЧИТАНИЕ СВЯТИТЕЛЯ ИННОКЕНТИЯ ИРКУТСКОГО В ДУХОВНОЙ КУЛЬТУРЕ РОССИИ: КНИЖНАЯ И РУКОПИСНАЯ ТРАДИЦИЯ (1805–1919 гг.)". НОВОСИБИРСК, 2009. - 536 с.
Authors: Кириченко Олег Викторович
Published: January 2022

Рецензент отмечает научную — практическую и теоретическую — важность появления фундаментальной монографии по столь значимой теме.
РЕЦЕНЗИЯ НА МОНОГРАФИЮ Т. А. ВОРОНИНОЙ "РУССКИЙ ПРАВОСЛАВНЫЙ ПОСТ: ОТ ПЕРВЫХ УСТАНОВЛЕНИЙ К СОВРЕМЕННОЙ ПРАКТИКЕ". М.: СОВРЕМЕННЫЕ ТЕТРАДИ, 2011. - 336 с.
Authors: Шляхтина Наталья Валерьевна
Published: January 2022

Рецензент отмечает научную — практическую и теоретическую — важность появления фундаментальной монографии по столь значимой теме народной религиозной культуры.
РУССКАЯ ЗЕМЛЯ, ОТЕЧЕСТВО, СВЯТАЯ РУСЬ, РОДИНА КАК ОБОЗНАЧЕНИЯ ЭТНИЧЕСКОГО ПРОСТРАНСТВА РУССКИХ
Authors: Кириченко Олег Викторович
Published: January 2022

Статья посвящена исследованию понятий, отвечающих за этничность, нагруженных общими этническими смыслами; эволюции понятий и модернизации их в имперский и советский периоды.
КРАТКИЙ ОТЧЕТ УЧАСТНИКА РОССИЙСКОЙ АКАДЕМИЧЕСКОЙ ГРУППЫ О ПРАЗДНОВАНИИ 200-ЛЕТИЯ ФОРТ-РОССА В КАЛИФОРНИИ
Authors: Кириченко Олег Викторович
Published: January 2022

В публикации дано описание событий, связанных с торжеством.
Казнь мучеников за веру Христову
Authors: Кириченко Олег Викторович
Published: January 2022

Публикация с комментариями архивного документа «Казнь мучеников за веру Христову», где глазами простой воронежской горожанки 1920‑х годов описываются события закрытия мужского Благовещенского монастыря.
ВОСПОМИНАНИЯ О СТРОИТЕЛЬСТВЕ СЕЛЬСКОГО ХРАМА ВОСКРЕСЕНИЯ ХРИСТОВА В С. БИТЮГ-МАТРЕНОВКА ВОРОНЕЖСКОЙ ОБЛ. А.А. ИВАНОВОЙ (МОНАХИНИ МИТРОФАНИИ)
Authors: Кириченко Олег Викторович
Published: January 2022

В публикации речь идет о строительстве пожилой москвичкой, внучкой репрессированного в советские годы священника, храма в селе, после его разрушения в 1960 е годы. Описывается долгий и непростой путь — от сбора средств на улицах Москвы до организации самого строительства и поиска священников для храма. Окончание публикации в 22 номере журнала.
СОСЛОВНЫЙ МИР ПОСТРЕФОРМЕННОЙ РОССИИ ПОД ОГНЕМ ОБЩЕСТВЕННОЙ И РЕВОЛЮЦИОННОЙ КРИТИКИ
Authors: Кириченко Олег Викторович
Published: January 2022

Статья посвящена проблеме разрушения сословий, что осуществлялось в стране целенаправленно и было связано с общереволюционными процессами и борьбой со всеми традиционными институтами.
РЕЦЕНЗИЯ НА КНИГУ Г.А. РОМАНОВА "В КРЕСТНЫЙ ХОД ИДЕТ НАРОД: ВПЕЧАТЛЕНИЯ ПАЛОМНИКОВ О СОТНЯХ КРЕСТНЫХ ХОДОВ В РОССИИ, О КРЕСТНЫХ ХОДАХ В ЧЕСТЬ НОВОМУЧЕНИКОВ ВО ВРЕМЯ ЕЛИЗАВЕТИНСКОГО МАРШРУТА МОСКВА-УРАЛ-СИБИРЬ". М.: СОЮЗ ДИЗАЙН, ФОНД ЕСПО, 2017. -272 с.
Authors: Шляхтина Наталья Валерьевна
Published: January 2022

Автор рецензии раскрывает значение этого крупного труда для современного читателя, представляет работу, имеющую важное значение для исследования современной религиозной жизни православных христиан, народной традиции в ее современном звучании.
РЕЦЕНЗИЯ НА МОНОГРАФИЮ М.В. ЛЕСКИНЕН "ВЕЛИКОРОСС/ВЕЛИКОРУС. ИЗ ИСТОРИИ КОНСТРУИРОВАНИЯ ЭТНИЧНОСТИ. ВЕК XIX". М.: "ИНДРИК", 2016. - 680 с.
Authors: Кириченко Олег Викторович
Published: January 2022

В рецензии поднимается вопрос об ответственности современного ученого за нравственные оценки в отношении отдельных народов. Автор рецензии исследует истоки методологии автора, позволившие предвзято и тенденциозно отнестись к заявленной теме.
Interactive, Visual Simulation of a Spatio-Temporal Model of Gas Exchange in the Human Alveolus
Authors: Schmid Kerstin , Knote Andreas , Mück Alexander , Pfeiffer Keram , von Mammen Sebastian , Fischer Sabine C.
Published: January 2022

In interdisciplinary fields such as systems biology, good communication between experimentalists and theorists is crucial for the success of a project. Theoretical modeling in physiology usually describes complex systems with many interdependencies. On one hand, these models have to be grounded on experimental data. On the other hand, experimenters must be able to understand the interdependent complexities of the theoretical model in order to interpret the model’s results in the physiological context. We promote interactive, visual simulations as an engaging way to present theoretical models in physiology and to make complex processes tangible. Based on a requirements analysis, we developed a new model for gas exchange in the human alveolus in combination with an interactive simulation software named Alvin. Alvin exceeds the current standard with its spatio-temporal resolution and a combination of visual and quantitative feedback. In Alvin, the course of the simulation can be traced in a three-dimensional rendering of an alveolus and dynamic plots. The user can interact by configuring essential model parameters. Alvin allows to run and compare multiple simulation instances simultaneously. We exemplified the use of Alvin for research by identifying unknown dependencies in published experimental data. Employing a detailed questionnaire, we showed the benefits of Alvin for education. We postulate that interactive, visual simulation of theoretical models, as we have implemented with Alvin on respiratory processes in the alveolus, can be of great help for communication between specialists and thereby advancing research.
Residue-Specific Incorporation of the Non-Canonical Amino Acid Norleucine Improves Lipase Activity on Synthetic Polyesters
Authors: Haernvall Karolina , Fladischer Patrik , Schoeffmann Heidemarie , Zitzenbacher Sabine , Pavkov-Keller Tea , Gruber Karl , Schick Michael , Yamamoto Motonori , Kuenkel Andreas , Ribitsch Doris , Guebitz Georg M. , Wiltschi Birgit
Published: January 2022

Environmentally friendly functionalization and recycling processes for synthetic polymers have recently gained momentum, and enzymes play a central role in these procedures. However, natural enzymes must be engineered to accept synthetic polymers as substrates. To enhance the activity on synthetic polyesters, the canonical amino acid methionine in Thermoanaerobacter thermohydrosulfuricus lipase (TTL) was exchanged by the residue-specific incorporation method for the more hydrophobic non-canonical norleucine (Nle). Strutural modelling of TTL revealed that residues Met-114 and Met-142 are in close vicinity of the active site and their replacement by the norleucine could modulate the catalytic activity of the enzyme. Indeed, hydrolysis of the polyethylene terephthalate model substrate by the Nle variant resulted in significantly higher amounts of release products than the Met variant. A similar trend was observed for an ionic phthalic polyester containing a short alkyl diol (C5). Interestingly, a 50% increased activity was found for TTL [Nle] towards ionic phthalic polyesters containing different ether diols compared to the parent enzyme TTL [Met]. These findings clearly demonstrate the high potential of non-canonical amino acids for enzyme engineering.
Comprehensive and Integrative Analysis of Two Novel SARS-CoV-2 Entry Associated Proteases CTSB and CTSL in Healthy Individuals and Cancer Patients
Authors: Huang Yongbiao , Li Shiyu , Huang Shanshan , Tu Jingyao , Chen Xinyi , Xiao Lingyan , Liu Bo , Yuan Xianglin
Published: January 2022

More than 200 million people have been infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and 4 million deaths have been reported worldwide to date. Cathepsin B/cathepsin L (CTSB/L) are SARS-CoV-2 entry–associated proteases and facilitate SARS-CoV-2 to infect host cells. However, the expressions of CTSB/L in healthy individuals and cancer patients remain not fully elucidated yet. Here, we comprehensively profiled the expressions and distributions of CTSB/L in human normal tissues, cancer tissues, and cell lines. Moreover, we compared CTSB/L expressions between various cancers and matched normal tissues, and investigated their genetic alteration and prognostic values in pan-cancer. Finally, we also explored the correlation between CTSB/L expressions and immune infiltration. We found that CTSB was highly expressed in most tissues, and CTSL was highly expressed predominantly in the digestive, urinary, and respiratory systems, such as the lungs, liver and gallbladder, and kidney tissues in the translational level. Moreover, cancer patients may be more susceptible to SARS-CoV-2 infection. Our data suggested that CTSB/L are overexpressed in aerodigestive and genitourinary cancers when compared with that in matched normal tissues, and their expressions were closely related to the prognosis of some cancer types. Interestingly, CTSB/L expressions were significantly correlated with immune cell infiltration in manifold cancer tissues and their corresponding normal tissues. In conclusion, our study shows a comprehensive bioinformatic analysis of two important SARS-CoV-2 entry–related proteases, which could provide a potential indication on prevention of SARS-CoV-2 infection.
Innovative Bioprocess Strategies Combining Physiological Control and Strain Engineering of Pichia pastoris to Improve Recombinant Protein Production
Authors: Gasset Arnau , Garcia-Ortega Xavier , Garrigós-Martínez Javier , Valero Francisco , Montesinos-Seguí José Luis
Published: January 2022

The combination of strain and bioprocess engineering strategies should be considered to obtain the highest levels of recombinant protein production (RPP) while assuring product quality and process reproducibility of heterologous products. In this work, two complementary approaches were investigated to improve bioprocess efficiency based on the yeast P. pastoris. Firstly, the performance of two Candida rugosa lipase 1 producer clones with different gene dosage under the regulation of the constitutive PGAP were compared in chemostat cultures with different oxygen-limiting conditions. Secondly, hypoxic conditions in carbon-limited fed-batch cultures were applied by means of a physiological control based on the respiratory quotient (RQ). Stirring rate was selected to maintain RQ between 1.4 and 1.6, since it was found to be the most favorable in chemostat. As the major outcome, between 2-fold and 4-fold higher specific production rate (qP) values were observed when comparing multicopy clone (MCC) and single-copy clone (SCC), both in chemostat and fed-batch. Additionally, when applying oxygen limitation, between 1.5-fold and 3-fold higher qP values were obtained compared with normoxic conditions. Thus, notable increases of up to 9-fold in the production rates were reached. Furthermore, transcriptional analysis of certain key genes related to RPP and central carbon metabolism were performed. Results seem to indicate the presence of a limitation in post-transcriptional protein processing steps and a possible transcription attenuation of the target gene in the strains with high gene dosage. The entire approach, including both strain and bioprocess engineering, represents a relevant novelty involving physiological control in Pichia cell factory and is of crucial interest in bioprocess optimization, boosting RPP, allowing bioproducts to be economically competitive in the market, and helping develop the bioeconomy.
Dual Functionalized Lactococcus lactis Shows Tumor Antigen Targeting and Cytokine Binding in Vitro
Authors: Zahirović Abida , Plavec Tina Vida , Berlec Aleš
Published: January 2022

Pro-inflammatory cytokines play an important role in the development and progression of colorectal cancer (CRC). Tumor-targeting bacteria that can capture pro-inflammatory cytokines in the tumor microenvironment and thus block their tumor-promoting effects might provide clinical benefits in inflammation-associated CRC. The aim of this study was to develop bacteria with dual functionality for selective delivery of cytokine-binding proteins to the tumor by targeting specific receptors on cancer cells. We engineered a model lactic acid bacterium, Lactococcus lactis, to co-display on its surface a protein ligand for tumor antigens (EpCAM-binding affitin; HER2-binding affibody) and a ligand for pro-inflammatory cytokines (IL-8-binding evasin; IL-6-binding affibody). Genes that encoded protein binders were cloned into a lactococcal dual promoter plasmid, and protein co-expression was confirmed by Western blotting. To assess the removal of IL-8 and IL-6 by the engineered bacteria, we established inflammatory cell models by stimulating cytokine secretion in human colon adenocarcinoma cells (Caco-2; HT-29) and monocyte-like cells (THP-1; U-937). The engineered L. lactis removed considerable amounts of IL-8 from the supernatant of Caco-2 and HT-29 cells, and depleted IL-6 from the supernatant of THP-1 and U-937 cells as determined by ELISA. The tumor targeting properties of the engineered bacteria were evaluated in human embryonic kidney epithelial cells HEK293 transfected to overexpress EpCAM or HER2 receptors. Fluorescence microscopy revealed that the engineered L. lactis specifically adhered to transfected HEK293 cells, where the EpCAM-targeting bacteria exhibited greater adhesion efficiency than the HER2-targeting bacteria. These results confirm the concept that L. lactis can be efficiently modified to display two proteins simultaneously on their surface: a tumor antigen binder and a cytokine binder. Both proteins remain biologically active and provide the bacteria with tumor antigen targeting and cytokine binding ability.
A Survey on the Current Status of Ophthalmological Consultations in Patients With Diabetes Undergoing Maintenance Hemodialysis and the Effectiveness of Education on Consultation Behavior –Experience of a Single Hemodialysis Clinic in Japan
Authors: Kimura Moritsugu , Toyoda Masao , Saito Nobumichi , Abe Makiko , Kato Eri , Sugihara Akemi , Ishida Naoto , Fukagawa Masafumi
Published: January 2022

Introduction

It is extremely important for patients with diabetes undergoing maintenance hemodialysis (MHD) to receive regular ophthalmologic examinations. However, even in the field of MHD in Japan, where there are many hemodialysis patients and the survival rate is said to be one of the highest in the world, we often see patients with diabetes who do not receive regular ophthalmologic examinations. In this study, we surveyed the status of ophthalmology consultations and the use of diabetic eye notebook (DEN) among hemodialysis patients with diabetes at hemodialysis clinics to confirm the current situation, with the aim of confirming the effectiveness of education on consultation behavior by medical care staff.

Materials and Methods

This study included 38 diabetic hemodialysis patients attending one MHD clinic in Japan for one year from March 2018 to March 2019. In the first fact-finding survey in March 2018, hemodialysis care unit nurses (HCUNs) in the hemodialysis unit asked the diabetic hemodialysis patients whether they had consulted an ophthalmologist and used the DEN. Based on the results, the HCUNs recommended that hemodialysis patients with complications of diabetes be educated about the usefulness of regular ophthalmologic examinations, even during MHD, and that they use the DEN. This was followed by a second fact-finding survey in March 2019 to reconfirm ophthalmology consultations and DEN use.

Results

Regarding the presence of ophthalmology consultations, 22 of 38 (58%) patients had regular ophthalmology consultations in March 2018, and 27 of 38 (71%) patients had consultations in the following year after receiving information from an HCUN. Only 1 of 22 patients (5%) who consulted the ophthalmologist in March 2018 used a DEN, but 19 of 27 patients (70%) used it the following year.

Conclusion

In the future, the development and utilization of a new DEN that includes more detailed patient information, and the spread of self-care guidance to patients by multidisciplinary health care professionals, will increase the consultation rate of MHD patients in Japan and reduce the incidence and progression of ocular diseases in MHD patients.
The Role of the Microbiota in Regeneration-Associated Processes
Authors: Díaz-Díaz Lymarie M. , Rodríguez-Villafañe Andrea , García-Arrarás José E.
Published: January 2022

The microbiota, the set of microorganisms associated with a particular environment or host, has acquired a prominent role in the study of many physiological and developmental processes. Among these, is the relationship between the microbiota and regenerative processes in various organisms. Here we introduce the concept of the microbiota and its involvement in regeneration-related cellular events. We then review the role of the microbiota in regenerative models that extend from the repair of tissue layers to the regeneration of complete organs or animals. We highlight the role of the microbiota in the digestive tract, since it accounts for a significant percentage of an animal microbiota, and at the same time provides an outstanding system to study microbiota effects on regeneration. Lastly, while this review serves to highlight echinoderms, primarily holothuroids, as models for regeneration studies, it also provides multiple examples of microbiota-related interactions in other processes in different organisms.
Segmentally Demineralized Cortical Bone With Stem Cell-Derived Matrix Promotes Proliferation, Migration and Differentiation of Stem Cells in vitro
Authors: He Shu-Kun , Ning Liang-Ju , Hu Ruo-Nan , Yao Xuan , Cui Jing , Ding Wei , Luo Jing-Cong , Qin Ting-Wu
Published: January 2022

A recent study has shown that demineralized cortical bone (DCB) did not improve the healing of tendon-bone interface. Considering that there is a gradient of mineral content in the tendon-bone interface, we designed a segmentally demineralized cortical bone (sDCB) scaffold with two different regions: undemineralized cortical bone section within the scaffold (sDCB-B) and complete demineralized cortical bone section within the scaffold (sDCB-D), to mimic the natural structure of the tendon-bone interface. Furthermore, the extracellular matrix (ECM) from tendon-derived stem cells (TDSCs) was used to modify the sDCB-D region of sDCB to construct a novel scaffold (sDCB-ECM) for enhancing the bioactivity of the sDCB-D. The surface topography, elemental distribution, histological structure, and surface elastic modulus of the scaffold were observed using scanning electron microscopy, energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy, histological staining and atomic force microscopy. Cell proliferation of bone marrow mesenchymal stem cells (BMSCs) and TDSCs cultured on scaffolds was evaluated using the Cell Counting kit-8, and cell viability was assessed by Live/Dead cell staining. Cell morphology was detected by fluorescent staining. The ability of the scaffolds to recruit stem cells was tested using transwell migration assay. The expression levels of bone-, cartilage- and tendon-related genes and proteins in stem cells were assessed by the polymerase chain reaction and western blotting. Our results demonstrated that there was a gradient of Ca and P elements in sDCB, and TDSC-derived ECM existed on the surface of the sDCB-D region of sDCB. The sDCB-ECM could promote stem cell proliferation and migration. Moreover, the sDCB-B region of sDCB-ECM could stimulate osteogenic and chondrogenic differentiation of BMSCs, and the sDCB-D-ECM region of sDCB-ECM could stimulate chondrogenic and tenogenic differentiation of TDSCs when compared to DCB. Our study indicated that sDCB-ECM might be a potential bioscaffold to enhance the tendon-bone interface regeneration.
Turning the Curve Into Straight: Phenogenetics of the Spine Morphology and Coordinate Maintenance in the Zebrafish
Authors: Muñoz-Montecinos Carlos , Romero Adrián , Sepúlveda Vania , Vira María Ángela , Fehrmann-Cartes Karen , Marcellini Sylvain , Aguilera Felipe , Caprile Teresa , Fuentes Ricardo
Published: January 2022

The vertebral column, or spine, provides mechanical support and determines body axis posture and motion. The most common malformation altering spine morphology and function is adolescent idiopathic scoliosis (AIS), a three-dimensional spinal deformity that affects approximately 4% of the population worldwide. Due to AIS genetic heterogenicity and the lack of suitable animal models for its study, the etiology of this condition remains unclear, thus limiting treatment options. We here review current advances in zebrafish phenogenetics concerning AIS-like models and highlight the recently discovered biological processes leading to spine malformations. First, we focus on gene functions and phenotypes controlling critical aspects of postembryonic aspects that prime in spine architecture development and straightening. Second, we summarize how primary cilia assembly and biomechanical stimulus transduction, cerebrospinal fluid components and flow driven by motile cilia have been implicated in the pathogenesis of AIS-like phenotypes. Third, we highlight the inflammatory responses associated with scoliosis. We finally discuss recent innovations and methodologies for morphometrically characterize and analyze the zebrafish spine. Ongoing phenotyping projects are expected to identify novel and unprecedented postembryonic gene functions controlling spine morphology and mutant models of AIS. Importantly, imaging and gene editing technologies are allowing deep phenotyping studies in the zebrafish, opening new experimental paradigms in the morphometric and three-dimensional assessment of spinal malformations. In the future, fully elucidating the phenogenetic underpinnings of AIS etiology in zebrafish and humans will undoubtedly lead to innovative pharmacological treatments against spinal deformities.
STIL Acts as an Oncogenetic Driver in a Primary Cilia-Dependent Manner in Human Cancer
Authors: Li Jingxian , Yang Zikun , Qi Yuanjiong , Liu Xun , Liu Yang , Gao Xinyu , Li Shuai , Zhu Jianqiang , Zhang Changwen , Du E , Zhang Zhihong
Published: January 2022

SCL/TAL1 Interrupting locus (STIL) is a ciliary-related gene involved in regulating the cell cycle and duplication of centrioles in dividing cells. STIL has been found disordered in multiple cancers and driven carcinogenesis. However, the molecular mechanisms and biological functions of STIL in cancers remain ambiguous. Here, we systematically analyzed the genetic alterations, molecular mechanisms, and clinical relevance of STIL across >10,000 samples representing 33 cancer types in The Cancer Genome Atlas (TCGA) dataset. We found that STIL expression is up-regulated in most cancer types compared with their adjacent normal tissues. The expression dysregulation of STIL was affected by copy number variation, mutation, and DNA methylation. High STIL expression was associated with worse outcomes and promoted the progression of cancers. Gene Ontology (GO) enrichment analysis and Gene Set Variation Analysis (GSVA) further revealed that STIL is involved in cell cycle progression, Mitotic spindle, G2M checkpoint, and E2F targets pathways across cancer types. STIL expression was negatively correlated with multiple genes taking part in ciliogenesis and was positively correlated with several genes which participated with centrosomal duplication or cilia degradation. Moreover, STIL silencing could promote primary cilia formation and inhibit cell cycle protein expression in prostate and kidney cancer cell lines. The phenotype and protein expression alteration due to STIL silencing could be reversed by IFT88 silencing in cancer cells. These results revealed that STIL could regulate the cell cycle through primary cilia in tumor cells. In summary, our results revealed the importance of STIL in cancers. Targeting STIL might be a novel therapeutic approach for cancers.
Enhanced Thermoelectric Properties of Composites Prepared With Poly(3,4-Ethylenedioxythiophene) Poly(Styrenesulfonate) and Vertically Aligned Se Wire
Authors: Kim In Yea , Chun Dong Won , Kim Sang-Il , Lim Jae-Hong
Published: January 2022

Controlling the electronic transport behavior in thermoelectric composites is one of the most promising approaches to enhance their power factor because this enables decoupling of the correlation between the electrical conductivity and Seebeck coefficient. Herein, we show that the unexpected high power factor of the Se nanowire array embedded in poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS) can be achieved by controlling the interfacial band structure engineering. The electrical conductivity and Seebeck coefficient simultaneously increased, confirming that the synthesis of organic/inorganic hybrid thermoelectric materials with improved performance was possible. Our exploration can be helpful for the rational design of high-performance thermoelectric composites through interface engineering.
Tetraphenylethene-Modified Colorimetric and Fluorescent Chemosensor for Hg2+ With Aggregation-Induced Emission Enhancement, Solvatochromic, and Mechanochromic Fluorescence Features
Authors: Tian Jin-jin , Deng Dian-dian , Wang Long , Chen Zhao , Pu Shouzhi
Published: January 2022

A tetraphenylethene (TPE)-modified rhodanine derivative was successfully designed and prepared, and this luminophor showed intramolecular charge transfer nature from the TPE unit to the rhodanine-3-acetic acid unit. Interestingly, this luminogen not only exhibited typical aggregation-induced emission enhancement (AIEE) behavior but also showed good cell imaging performance. Remarkably, this AIEE-active TPE-containing rhodanine derivative possessed noticeable solvatochromic fluorescence effect involving multiple fluorescent colors of green, yellow-green, yellow, orange, and red. Meanwhile, this fluorescigenic compound displayed reversible mechanochromic fluorescence behavior based on the mutual transformation of between stable crystalline and metastable amorphous states. On the other hand, this multifunctional fluorophor could selectively and sensitively detect Hg2+ in an acetonitrile solution. Furthermore, this chemosensor could also be used to detect Hg2+ on test paper strips.
Recent Advances in Simple Preparation of 3D Graphene Aerogels Based on 2D Graphene Materials
Authors: Ding Meichun , Li Chenwei
Published: January 2022

Recently, 3D graphene aerogels (3GAs) with high electrical conductivity, excellent mechanical properties, and fast mass and electron transport have attracted increasing attention and shown wide applications (such as flexible electronics devices, sensors, absorbents, catalysis, energy storage devices, solar steam generation devices, and so on). The drying process becomes an important factor limiting the large-scale preparation of 3GAs. Therefore, how to simplify the preparation process plays an important role in the large-scale application of 3GAs. In this study, we summarize the recent progresses of 3GAs by different drying methods and focus on the effect of robust graphene network on the simple preparation of 3GAs. Besides, the design and synthesis strategies of 3GAs with robust graphene network structures have been systematically discussed. Finally, the emerging challenges and prospective for developing simple preparation and functionalization of 3GAs were outlined. It is expected that our study will lay a foundation for large-scale preparation and application of 3GAs and inspire more new ideas in this field.
Adjustment of the GRACE Risk Score by Monocyte to High-Density Lipoprotein Ratio Improves Prediction of Adverse Cardiovascular Outcomes in Patients With Acute Coronary Syndrome Undergoing Percutaneous Coronary Intervention
Authors: Ma Xiaoteng , Han Kangning , Yang Lixia , Shao Qiaoyu , Li Qiuxuan , Wang Zhijian , Li Yueping , Gao Fei , Yang Zhiqiang , Shi Dongmei , Zhou Yujie
Published: January 2022

Background and Aims

The monocyte to high-density lipoprotein cholesterol ratio (MHR), a novel marker for inflammation and lipid metabolism, has been demonstrated to be associated with poor prognosis in many patient populations. However, the prognostic influence of MHR in patients with acute coronary syndrome (ACS) undergoing percutaneous coronary intervention (PCI) is poorly understood. Here, we sought to investigate the relationship between MHR and adverse cardiovascular (CV) outcomes in such patients and determine whether MHR could improve the GRACE risk score based prognostic models.

Methods and Results

MHR was applied to 1,720 patients with ACS undergoing PCI who were admitted to our CV center from June 2016 to November 2017. These patients were stratified into three groups according to MHR tertiles. The relationship between MHR and the primary endpoint (overall death, non-fatal stroke, non-fatal myocardial infarction, or unplanned repeat revascularization) was examined by Cox proportional hazards regression analysis. During a median follow-up of 31 months, 353 patients had at least one primary endpoint event. Compared with those in the lowest MHR tertile, patients in the middle and highest tertiles [adjusted HR: 1.541 (95% CI: 1.152–2.060) and 1.800 (95%CI: 1.333–2.432), respectively], had a higher risk of the primary endpoint. The addition of MHR has an incremental effect on the predictive ability of the GRACE risk score for the primary endpoint (cNRI: 0.136, P < 0.001; IDI: 0.006, P < 0.001).

Conclusion

MHR was independently and significantly associated with adverse CV outcomes in ACS patients who underwent PCI and improved the predictive ability of the GRACE risk score based prognostic models.

Registration Number

http://www.chictr.org.cn/hvshowproject.aspx?id=21397; ChiCTR1800017417.