An application of THz gas high resolution spectroscopy method for investigation of thermal decomposition products of biological liquid (urine) of rats with dysbacteriosis
Authors:   IPM RAS, 603950, Russia, Nizhny Novgorod, GSP-105, Vaks V.L. ,   Lobachevsky University, 603022, Russia, Nizhny Novgorod, Gagarina av., 23, Domracheva E.G. ,   IPM RAS, 603950, Russia, Nizhny Novgorod, GSP-105,   Lobachevsky University, 603022, Russia, Nizhny Novgorod, Gagarina av., 23, Chernyaeva M.B. ,   IPM RAS, 603950, Russia, Nizhny Novgorod, GSP-105,   Lobachevsky University, 603022, Russia, Nizhny Novgorod, Gagarina av., 23, Anfertev V.A. ,   IPM RAS, 603950, Russia, Nizhny Novgorod, GSP-105,   Lobachevsky University, 603022, Russia, Nizhny Novgorod, Gagarina av., 23, Zhukova E.S. ,   Nizhny Novgorod research institute for hygiene and occupational pathology, Rospotrebnadzor, 603005, Russia, Nizhny Novgorod, Semashko str., 20, Khramov R.N. ,   Institute of Theoretical and Experimental Biophysics RAS, 142290, Russia, Pushchino, Institutskaya, 3, Gapeyev A.B. ,   Institute of Cell Biophysics RAS, 142290, Russia, Pushchino, Institutskaya, 3, Shcherbatyuk T.G. ,   Nizhny Novgorod research institute for hygiene and occupational pathology, Rospotrebnadzor, 603005, Russia, Nizhny Novgorod, Semashko str., 20
Published: December 2022

The trend towards diagnostic approach based on revealing the metabolic profile of disease is observed in current medicine. The metabolic profile of the disease is a set of metabolites – final or intermediate products of metabolism in the living body, specific for a given disease or pathology. The search for biomarkers is possible in exhaled breath, as well as in biological liquids, and last ones are often more informative, because the liquids respond fast to changes of biological organism state and substances may be present in higher concentrations than gas excretions of organism. Preclinical trials of approaches to the treatment of diseases, including socially significant ones, such as dysbacteriosis, are carries out on rodents. The thermal decomposition products content of the line of the urine samples of rats with artificially induced dysbacteriosis was studied in this work. A promising approach for studying multicomponent gas mixtures of various origins, including biological ones, is molecular absorption spectroscopy, in particular, nonstationary spectroscopy in the terahertz (THz) frequency range. When radiation that has passed through a gaseous sample is detected, absorption lines are recorded in the spectrum, which are an unambiguous characteristic of specific substances. By the presence of these absorption lines, the conclusion about the presence of these substances in the studied multicomponent gas mixture can be done, and, therefore, potential markers of pathologies, diseases and markers that characterize the effects of various effects on a living organism, can be identified. A set of metabolites appeared during thermal decomposition of urine samples of rodent (healthy, with artificially induced dysbacteriosis, with various treatment regimens) was revealed, and the compositions of gaseous products were compared. The presented approach is promising for the development of a non-invasive research method that allows to identify markers that reflect the presence of dysbacteriosis, as well as to assess the impact on the rat’s organism during the therapy.