bluuebunny/crossref_metadata_2025 huggingface dataset:
Cucurbitacin-B instigates intrinsic apoptosis and modulates Notch signaling in androgen-dependent prostate cancer LNCaP cells
Authors: Alafnan Ahmed , Khalifa Nasrin E. , Hussain Talib , Osman Mhdia Elhadi
Published: June 2023

Introduction: Among numerous triterpenoids of the Cucurbitaceae family, Cucurbitacin-B (Cur-B) is being explored for its pharmacological attributes. Reports from previous studies have explicitly shown that Cur-B possesses substantial anticancer effects. The present report focuses on exploring the anticancer attributes of Cur-B against androgen-dependent PCa LNCaP cells.

Methods: LNCaP cells were exposed to commercially available purified Cur-B at varying concentrations that were selected as 5, 10, 15, 20, and 25 µM for some time of 24 h to perform various experimental studies.

Results: Cytotoxicity evaluation revealed that Cur-B impeded the LNCaP cell’s viability at 5 µM (p <0.05) which increased considerably at a concentration of 25 µM (p <0.001). Cur-B was also efficacious in inducing the changes within nu-clear morphology followed by a concomitant increase in the activities of key caspases including caspase-3, -8, and -9 intriguingly in a dose-dependent trend. Cur-B treatment not only resulted in the augmentation of intracellular ROS levels within LNCaP cells at 5 µM (p <0.05) but also in-creased significantly at 25 µM concentration (p <0.001). Elevation in the ROS levels was also found to be correlated with dissipated mitochondrial membrane potential (ΔΨm) which culminated in the onset of significant apoptosis at 25 µM concentration (p <0.001). Cur-B exposure also resulted in the downregulation of cyclin D1, cyclin-dependent kinase 4 (CDK4) followed by amplified levels of p21Cip1 mRNA. Importantly, exposure of Cur-B competently reduced the expression of the Notch signaling cascade which may be the plausible cause behind Cur-B-instigated apoptotic cell death and cell cycle arrest in LNCaP cells.

Discussion: These observations thus, explicitly indicated that Cur-B could be plausibly further explored as potent therapeutics against androgen-dependent PCa.
Sex differences in long-term effects of collagen-induced arthritis in middle-aged mice
Authors: Schuh Bernhard Maximilian , Macáková Kristína , Feješ Andrej , Groß Tim , Belvončíková Paulína , Janko Jakub , Juskanič Dominik , Hollý Samuel , Borbélyová Veronika , Šteňová Emőke , Pastorek Michal , Vlková Barbora , Celec Peter
Published: June 2023

Introduction: Rheumatoid arthritis (RA) is a chronic inflammatory disorder with high prevalence among middle-aged women. Collagen-induced arthritis (CIA) is the most widely used animal model of RA, however, sex differences and long-term effects of CIA in mice are poorly described in the literature.

Aim: Therefore, the present study aimed to analyze the long-term effects of CIA on the joints of middle-aged mice of both sexes and to describe potential sex differences.

Materials and methods: CIA was induced in middle-aged DBA/1J mice by immunization with bovine type II collagen and complete Freund’s adjuvant. Saline was administered to control mice. Arthritis score assessment, plethysmometry, and thermal imaging of the joints were performed weekly for 15 weeks. Locomotor activity, micro-computed tomography, joint histology and biochemical analyses were performed at the end of the experiment.

Results: Our results indicate a similar prevalence of arthritis in both sexes of mice—67% (8/12) of females and 89% (8/9) males with an earlier onset in males (day 14 vs. day 35). After the arthritis scores peaked on day 56 for males and day 63 for females, they steadily declined until the end of the experiment on day 105. A similar dynamics was observed in paw volume and temperature analyzing different aspects of joint inflammation. Long-term consequences including higher proteinuria (by 116%), loss of bone density (by 33.5%) and joint damage in terms of synovial hyperplasia as well as bone and cartilage erosions were more severe in CIA males compared to CIA females. There were no significant differences in locomotor activity between CIA mice and CTRL mice of any sex.

Conclusion: This is the first study to describe the long-term effects of the CIA model in terms of sex differences in DBA/1J mice. Our results indicate sex differences in the dynamics, but not in the extent of arthritis. An earlier onset of arthritis and more severe consequences on joints, bones and kidneys were found in males. The underlying immune pathomechanisms responsible for the limited duration of the arthritis symptoms and the opposite sex difference in comparison to RA patients require further investigation.
Diversity analysis of agronomic and nutritional traits of hybrid offspring of forage bermudagrass
Authors: Chen Jianmin , Fan Shugao , Li Shuang , Cui Xinyu , Amombo Erick , Ji Mingxia , Liu Xiaoyan , Fu Jinmin
Published: June 2023

Because of its excellent stress resistance and forage quality, the forage bermudagrass hybrid population had attracted the attention of scientific researchers in recent years. Studying its diversity could promote the breeding of desirable varieties. The variability in agronomic traits including fresh weight, dry weight, ash content, crude protein content, crude fat, phosphorus content, and relative feed value for 56 bermudagrass was investigated using Wrangler as an experimental reference. Grey correlation analysis and cluster analysis were employed to screen bermudagrass with high yield and superior quality. WCF-34 had the highest 2-year fresh weight (109,773.3 kg/ha), WCF-37 had the highest 2-year dry weight (31,951.6 kg/ha), WCF-24 had the lowest Ash content (7.46%), WCF-26 had the highest crude protein content (16.27%), WCF-27 had the highest curde fat content (3.58%), WCF-13 had the highest P content (0.45%), and WCF-42 had the highest relative feed value (95.32). Combining the results of grey relational analysis and cluster analysis, WCF-42, WCF-34, WCF-38, WCF-37, and WCF-40 were selected as high-quality bermudagrass. Through comprehensive analysis of the agronomic characters of bermudagrass, five bermudagrass were selected, the outcomes of this study would provide a theoretical basis for the breeding and genetic enhancement of bermudagrass.
Structural mechanism of heavy metal-associated integrated domain engineering of paired nucleotide-binding and leucine-rich repeat proteins in rice
Authors: Guo Liwei , Mu Yuanyu , Wang Dongli , Ye Chen , Zhu Shusheng , Cai Hong , Zhu Youyong , Peng Youliang , Liu Junfeng , He Xiahong
Published: June 2023

Plant nucleotide-binding and leucine-rich repeat (NLR) proteins are immune sensors that detect pathogen effectors and initiate a strong immune response. In many cases, single NLR proteins are sufficient for both effector recognition and signaling activation. These proteins possess a conserved architecture, including a C-terminal leucine-rich repeat (LRR) domain, a central nucleotide-binding (NB) domain, and a variable N-terminal domain. Nevertheless, many paired NLRs linked in a head-to-head configuration have now been identified. The ones carrying integrated domains (IDs) can recognize pathogen effector proteins by various modes; these are known as sensor NLR (sNLR) proteins. Structural and biochemical studies have provided insights into the molecular basis of heavy metal-associated IDs (HMA IDs) from paired NLRs in rice and revealed the co-evolution between pathogens and hosts by combining naturally occurring favorable interactions across diverse interfaces. Focusing on structural and molecular models, here we highlight advances in structure-guided engineering to expand and enhance the response profile of paired NLR-HMA IDs in rice to variants of the rice blast pathogen MAX-effectors (Magnaporthe oryzae AVRs and ToxB-like). These results demonstrate that the HMA IDs-based design of rice materials with broad and enhanced resistance profiles possesses great application potential but also face considerable challenges.
Development of Omni InDel and supporting database for maize
Authors: Liu Zhihao , Zhao Yikun , Zhang Yunlong , Xu Liwen , Zhou Ling , Yang Weiguang , Zhao Han , Zhao Jiuran , Wang Fengge
Published: June 2023

Insertions–deletions (InDels) are the second most abundant molecular marker in the genome and have been widely used in molecular biology research along with simple sequence repeats (SSR) and single-nucleotide polymorphisms (SNP). However, InDel variant mining and marker development usually focuses on a single type of dimorphic InDel, which does not reflect the overall InDel diversity across the genome. Here, we developed Omni InDels for maize, soybean, and rice based on sequencing data and genome assembly that included InDel variants with base lengths from 1 bp to several Mb, and we conducted a detailed classification of Omni InDels. Moreover, we screened a set of InDels that are easily detected and typed (Perfect InDels) from the Omni InDels, verified the site authenticity using 3,587 germplasm resources from 11 groups, and analyzed the germplasm resources. Furthermore, we developed a Multi-InDel set based on the Omni InDels; each Multi-InDel contains multiple InDels, which greatly increases site polymorphism, they can be detected in multiple platforms such as fluorescent capillary electrophoresis and sequencing. Finally, we developed an online database website to make Omni InDels easy to use and share and developed a visual browsing function called “Variant viewer” for all Omni InDel sites to better display the variant distribution.
The conscious processing of emotion in depression disorder: a meta-analysis of neuroimaging studies
Authors: Gou Xin-yun , Li Yu-xi , Guo Liu-xue , Zhao Jing , Zhong Dong-ling , Liu Xiao-bo , Xia Hai-sha , Fan Jin , Zhang Yue , Ai Shuang-chun , Huang Jia-xi , Li Hong-ru , Li Juan , Jin Rong-jiang
Published: June 2023

Background

Depression is generally accompanied by a disturbed conscious processing of emotion, which manifests as a negative bias to facial/voice emotion information and a decreased accuracy in emotion recognition tasks. Several studies have proved that abnormal brain activation was responsible for the deficit function of conscious emotion recognition in depression. However, the altered brain activation related to the conscious processing of emotion in depression was incongruent among studies. Therefore, we conducted an activation likelihood estimation (ALE) analysis to better understand the underlying neurophysiological mechanism of conscious processing of emotion in depression.

Method

Electronic databases were searched using the search terms “depression,” “emotion recognition,” and “neuroimaging” from inceptions to April 10th, 2023. We retrieved trials which explored the neuro-responses of depressive patients to explicit emotion recognition tasks. Two investigators independently performed literature selection, data extraction, and risk of bias assessment. The spatial consistency of brain activation in conscious facial expressions recognition was calculated using ALE. The robustness of the results was examined by Jackknife sensitivity analysis.

Results

We retrieved 11,365 articles in total, 28 of which were included. In the overall analysis, we found increased activity in the middle temporal gyrus, superior temporal gyrus, parahippocampal gyrus, and cuneus, and decreased activity in the superior temporal gyrus, inferior parietal lobule, insula, and superior frontal gyrus. In response to positive stimuli, depressive patients showed hyperactivity in the medial frontal gyrus, middle temporal gyrus, and insula (uncorrected p < 0.001). When receiving negative stimuli, a higher activation was found in the precentral gyrus, middle frontal gyrus, precuneus, and superior temporal gyrus (uncorrected p < 0.001).

Conclusion

Among depressive patients, a broad spectrum of brain areas was involved in a deficit of conscious emotion processing. The activation of brain regions was different in response to positive or negative stimuli. Due to potential clinical heterogeneity, the findings should be treated with caution.

Systematic review registration

https://inplasy.com/inplasy-2022-11-0057/, identifier: 2022110057.
One size (doesn’t) fit all: new metaphors for and practices of scaling from indigenous peoples of the Northwest Amazon
Authors: Shaw Kurt , da Silva Rita de Cacia Oenning
Published: June 2023

Ten years of field research and collaborative development of programs for early childhood in the Upper Rio Negro region of the Amazon provide the authors with new metaphors for achieving wider social impact and new frames to add to the international debate on ‘scaling’ social change initiatives. Using anthropology and ethno-ontology to think questions of universal and particular, center and periphery, the article reflects on the dangers of monolithic scaling to cultural diversity and future innovation. Instead of the metaphor of scaling — adopted in the discourse of public policy and international development from the Fordist or Taylorist efficiency of the economy of scale — indigenous people speak of exchange, sharing, and transformation. These ideas seek to connect local and decolonized models and value the diversity of local knowledges, epistemologies, and practices around early childhood development. Based on the expansion of the CanalCanoa project among diverse indigenous communities, the paper proposes a flexible and bottom-up model of achieving impact at scale through empowering local actors to teach each other and establish local criteria of learning and evaluation.
The relationship between self-cohesion and smartphone addiction: the mediating role of rejection sensitivity
Authors: El Keshky Mogeda El Sayed , Aseem Huda , Alzain Amira , Samak Yasser Abdelazim Abdelmawgoud
Published: June 2023

Background

The increasing use of smartphones with attractive applications has yielded concerns over problematic overuse, also called smartphone addiction, thus creating a need to investigate the antecedents and pathways of this addiction.

Objective

The aim of this study was to investigate the relationships between the self-cohesion dimensions of presence and consistency and smartphone addiction, and the potentially mediating role of rejection sensitivity.

Methods

A total sample of 910 respondents (58% females, mean age = 34.9, SD = 13.6) participated in the study. They completed a questionnaire including demographic characteristics and queries about frequency and duration of smartphone use, as well as the Self-Cohesion Scale, the Short Version of Smartphone Addiction Scale, and the Rejection Sensitivity RS-Adult Questionnaire. Structural equation modeling was used to examine the relationships in question.

Results

The findings indicated that smartphone addiction was positively correlated with rejection sensitivity and negatively correlated with the self-cohesion dimensions of presence and consistency. Further, both presence and consistency were negatively associated with smartphone addiction and rejection sensitivity negatively mediated these relationships.

Conclusion

Self-cohesion and rejection sensitivity appear to be important predictors of smartphone addiction. Efforts to counteract smartphone addiction should, therefore, endeavor to increase smartphone users’ self-cohesion and reduce their rejection sensitivity.
Increasing incidence of Plasmodium ovale and persistent reporting of Plasmodium vivax in imported malaria cases: an analysis of 9-year surveillance data in four areas of China
Authors: Wang Xiaoxiao , Xu Wenjie , Luo Fei , Lin Kangming , Zhang Tao , Yao Linong , Zhang Xuan , Zhang Jiaqi , Auburn Sarah , Wang Duoquan , Ruan Wei
Published: June 2023

Background

This study aimed at exploring the epidemiological pattern of imported malaria in China before malaria elimination in 2021, to provide evidence-based data for preventing malaria re-establishment in China.

Methods

Nine-year surveillance data on imported malaria in four provincial-level administrative divisions (PLADs) (Anhui, Chongqing, Guangxi, and Zhejiang) between 2011 and 2019 were thoroughly collected and analyzed.

Results

A quite stable trend in imported malaria cases between 2011 and 2019 was observed. In total, 6,064 imported patients were included. Plasmodium falciparum was the most frequently reported species (4,575, 75.6%). Cases of malaria were most frequently imported from Western Africa (54.4%). We identified an increasing trend in P. ovale and a persistence of P. vivax infections among the cases of malaria imported from Western Africa. Most patients (97.5%) were 20–50 years old. Among imported malaria infections, the main purposes for traveling abroad were labor export (4,914/6,064, 81.0%) and business trips (649, 10.7%). Most patients (2,008/6,064, 33.1%) first visited county-level medical institutions when they sought medical help in China. More patients were diagnosed within 3 days after visiting Centers for Disease Control and Prevention (CDCs) or entry–exit quarantine facilities (EQFs) (1,147/1609, 71.3%) than after visiting medical institutions (2,182/3993, 54.6%).

Conclusion

Imported malaria still poses a threat to the malaria-free status of China. County-level institutions are the primary targets in China to improve the sensitivity of the surveillance system and prevent the re-establishment of malaria. Health education should focus on exported labors, especially to Western and Central Africa. Increasing trend in P. ovale and persistence of P. vivax infections indicated their underestimations in Western Africa. Efficient diagnostic tools and sensitive monitoring systems are required to identify Plasmodium species in Africa.
Distributed rate control of smart solar arrays with batteries
Authors: Bovornkeeratiroj Phuthipong , Lee Stephen , Iyengar Srinivasan , Irwin David , Shenoy Prashant
Published: June 2023

Continued advances in technology have led to falling costs and a dramatic increase in the aggregate amount of solar capacity installed across the world. A drawback of increased solar penetration is the potential for supply-demand mismatches in the grid due to the intermittent nature of solar generation. While energy storage can be used to mask such problems, we argue that there is also a need to explicitly control the rate of solar generation of each solar array in order to achieve high penetration while also handling supply-demand mismatches. To address this issue, we present the notion of smart solar arrays that can actively modulate their solar output based on the notion of proportional fairness. We present a decentralized algorithm based on Lagrangian optimization that enables each smart solar array to make local decisions on its fair share of solar power it can inject into the grid and then present a sense-broadcast-respond protocol to implement our decentralized algorithm into smart solar arrays. We also study the benefits of using energy storage when we rate control solar. To do so, we present a decentralized algorithm to charge and discharge batteries for each smart solar. Our evaluation on a city-scale dataset shows that our approach enables 2.6× more solar penetration while causing smart arrays to reduce their output by as little as 12.4%. By employing an adaptive gradient approach, our decentralized algorithm has 3 to 30× faster convergence. Finally, we demonstrate energy storage can help netmeter more solar energy while ensuring fairness and grid constraints are met.
An embarrassingly simple approach for visual navigation of forest environments
Authors: Niu Chaoyue , Newlands Callum , Zauner Klaus-Peter , Tarapore Danesh
Published: June 2023

Navigation in forest environments is a challenging and open problem in the area of field robotics. Rovers in forest environments are required to infer the traversability of a priori unknown terrains, comprising a number of different types of compliant and rigid obstacles, under varying lighting and weather conditions. The challenges are further compounded for inexpensive small-sized (portable) rovers. While such rovers may be useful for collaboratively monitoring large tracts of forests as a swarm, with low environmental impact, their small-size affords them only a low viewpoint of their proximal terrain. Moreover, their limited view may frequently be partially occluded by compliant obstacles in close proximity such as shrubs and tall grass. Perhaps, consequently, most studies on off-road navigation typically use large-sized rovers equipped with expensive exteroceptive navigation sensors. We design a low-cost navigation system tailored for small-sized forest rovers. For navigation, a light-weight convolution neural network is used to predict depth images from RGB input images from a low-viewpoint monocular camera. Subsequently, a simple coarse-grained navigation algorithm aggregates the predicted depth information to steer our mobile platform towards open traversable areas in the forest while avoiding obstacles. In this study, the steering commands output from our navigation algorithm direct an operator pushing the mobile platform. Our navigation algorithm has been extensively tested in high-fidelity forest simulations and in field trials. Using no more than a 16 × 16 pixel depth prediction image from a 32 × 32 pixel RGB image, our algorithm running on a Raspberry Pi was able to successfully navigate a total of over 750 m of real-world forest terrain comprising shrubs, dense bushes, tall grass, fallen branches, fallen tree trunks, small ditches and mounds, and standing trees, under five different weather conditions and four different times of day. Furthermore, our algorithm exhibits robustness to changes in the mobile platform’s camera pitch angle, motion blur, low lighting at dusk, and high-contrast lighting conditions.
High-resolution permittivity estimation of ground penetrating radar data by migration with isolated hyperbolic diffractions and local focusing analyses
Authors: Lv Wenmin , Zhang Jinhai
Published: June 2023

Ground penetrating radar (GPR) is important for detecting shallow subsurface structures, which has been successfully used on the Earth, Moon, and Mars. It is difficult to analyze the underground permittivity from GPR data because its observation system is almost zero-offset. Traditional velocity analysis methods can work well with separable diffractions but fail with strong-interfered diffractions. However, in most situations, especially for lunar or Martian exploration, the diffractions are highly interfered, or even buried in reflections. Here, we proposed a new method to estimate the underground permittivity and apply it to lunar penetrating radar data. First, we isolate a group of diffractions with a hyperbolic time window determined by a given velocity. Then, we perform migration using the given velocity and evaluate the focusing effects of migration results. Next, we find the most focused results after scanning a series of velocities and regard the corresponding velocity as the best estimation. Finally, we assemble all locally focused points and derive the best velocity model. Tests show that our method has high spatial resolution and can handle strong noises, thus can achieve velocity analyses with high accuracy, especially for complex materials. The permittivity of lunar regolith at Chang’E-4 landing area is estimated to be ∼4 within 12 m, ranging from 3.5 to 4.2 with a local perturbation of ∼2.3%, consistent with ∼3% obtained by numerical simulations using self-organization random models. This suggests that the lunar regolith at Chang’E-4 landing area is mature and can be well described by self-organization random models.
A novel triplex real-time PCR assay for the differentiation of lumpy skin disease virus, goatpox virus, and sheeppox virus
Authors: Nan Wenlong , Gong Mingxia , Lu You , Li Jinming , Li Lin , Qu Hailong , Liu Chunju , Wang Ying , Wu Faxing , Wu Xiaodong , Wang Zhiliang , Chen Yiping , Peng Daxin
Published: June 2023

Introduction

Three members of Capripoxvirus (CaPV) genus, including lumpy skin disease virus (LSDV), goatpox virus (GTPV), and sheeppox virus (SPPV), are mentioned as notifiable forms by World Organization for Animal Health. These viruses have negatively impacted ruminant farming industry worldwide, causing great economic losses. Although SPPV and GTPV cause more severe clinical disease in only one animal species, they can transfer between sheep and goats. Both homologous and heterologous immunization strategies are used to protect animals against CaPVs. However, development of accurate and rapid methods to distinguish these three viruses is helpful for the early detection, disease surveillance, and control of CaPV infection. Therefore, we developed a novel triplex real-time PCR (qPCR) for the differentiation of LSDV, GTPV, and SPPV.

Methods

Universal primers were designed to detect pan-CaPV sequences. Species-specific minor groove binder (MGB)-based probes were designed, which were labeled with FAM for LSDV, HEX for GTPV, and ROX for SPPV. The sensitivity, specificity, reproducibility, and ability of detecting mixed infections were evaluated for the triplex qPCR. Further, 226 clinical samples of the infection and negative controls were subjected to the triplex qPCR, and the results were verified using PCR-restriction fragment length polymorphism (PCR-RFLP) and sequencing methods for PRO30 gene.

Results

The triplex qPCR could successfully distinguish LSDV, GTPV, and SPPV in one reaction, and the assay sensitivity was 5.41, 27.70, and 17.28 copies/μL, respectively. No cross-reactivity was observed with other viruses causing common ruminant diseases, including des petits ruminants virus, foot-and-mouth disease virus, bluetongue virus, ovine contagious pustular dermatitis virus, infectious bovine rhinotracheitis virus, and bovine viral diarrhea-mucosal disease virus. Inter-and intra-assay variabilities were < 2.5%. The results indicated that the triplex qPCR was highly specific, sensitive, and reproducible. Simulation experiments revealed that this assay could successfully distinguish two or three viruses in case of mixed infections without any cross-reaction. For clinical samples, the results were completely consistent with the results of PCR-RFLP and sequencing. This demonstrated that the assay was reliable for clinical application.

Discussion

The triplex qPCR is a robust, rapid, and simple tool for identifying various types of CaPV as it can successfully distinguish LSDV, GTPV, and SPPV in one reaction. Furthermore, the assay can facilitate more accurate disease diagnosis and surveillance for better control of CaPV infection.
Construction of a one-step multiplex real-time PCR assay for the detection of serogroups A, B, and E of Pasteurella multocida associated with bovine pasteurellosis
Authors: Wang Haojie , Xin Lingxiang , Wu Yang , Liu Yan , Yao Wensheng , Zhang He , Hu Yunhao , Tong Rendong , Zhu Liangquan
Published: June 2023

Bovine pasteurellosis, caused by serogroups A, B, and E of Pasteurella multocida (Pm), is mainly manifested as bovine respiratory disease (BRD) and hemorrhagic septicemia (HS). The disease has caused a great economic loss for the cattle industry globally. Therefore, identifying the Pm serogroups is critical for optimal diagnosis and subsequent clinical treatment and even epidemiological studies. In this study, a one-step multiplex real-time PCR assay was established. Three pairs of specific primers were prepared to detect the highly conserved genomic regions of serogroups A (HyaD), B (bcbD), and E (ecbJ) of Pm, respectively. The results depicted that the method had no cross-reaction with other bovine pathogens (Mannheimia hemolytica, Escherichia coli, Listeria monocytogenes, Staphylococcus aureus, Salmonella Dublin, Mycobacterium paratuberculosis, infectious bovine rhinotracheitis virus, and Mycoplasma bovis). The linear range (107 to 102 copies/μL) showed the R2 values for serogroups A, B, and E of Pm as 0.9975, 0.9964, and 0.996, respectively. The multiplex real-time PCR efficiency was 90.30%, 90.72%, and 90.57% for CartA, CartB, and CartE, respectively. The sensitivity result showed that the serogroups A, B, and E of Pm could be detected to be as low as 10 copies/μL. The repeatability result clarified that an intra-assay and an inter-assay coefficient of variation of serogroups A, B, and E of Pm was < 2%. For the clinical samples, the detection rate was higher than the OIE-recommended ordinary PCR. Overall, the established one-step multiplex real-time PCR assay may be a valuable tool for the rapid and early detection of the serogroups A, B, and E of Pm with high specificity and sensitivity.
Menstrual Symptoms: Insights from Mobile Menstrual Tracking Applications for English and Chinese Teenagers
Authors: Peng Sisi , Yang Yuyin , Haselton Martie G.
Published: June 2023

Mobile software applications (apps) have transformed how individuals oversee and maintain their own health. One way that girls can monitor their menstrual cycles is through the increasingly widespread use of mobile menstrual tracking apps. This study aimed to examine menstrual symptom tracking for adolescents in English and Chinese apps, exploring menstrual literacy, cross-cultural differences, and framing, or presentation, of symptoms. The mixed-methods content analysis involved 15 popular free menstrual tracking apps in English (n = 8) and Chinese (n = 7), sampled from December 2022 to January 2023. A quantitative analysis of qualitative data was conducted through manual coding of content and automatically analyzing sentiment, or emotional tone, using a computational approach. We found that (1) menstrual literacy on symptom management or treatment was generally insufficient, (2) there were more available emotional than physical symptoms in English than Chinese apps, and (3) symptoms were framed more negatively than positively somewhat more in Chinese than English apps. Our findings emphasize the urgency to provide better evidence-informed communication about symptoms, either presented more positively or neutrally, in menstrual tracking apps for adolescent users. Since adolescence is a critical developmental stage that requires ample support, we recommend that digital menstrual trackers be crucially improved and future research should investigate how they can uniquely shape attitudes and experiences, and subsequent sexual and reproductive health empowerment and bodily autonomy.
Experimental and Numerical Study on the Combined Jet Impingement and Film Cooling of an Aero-Engine Afterburner Section
Authors: Singh Ashutosh , Kumar Sourabh , Singh Kuldeep
Published: June 2023

The recent advancement of cooling methodologies for critical components such as turbine blades, combustor liners, and afterburner liners has led to the development of a combination of impingement and film cooling. The present study proposes an efficient cooling technique for a modern aero-engine afterburner liner based on the combination of jet impingement and film cooling. To achieve this, a numerical model is devised to model the film flow over a corrugated liner with several jets impinging over it. The numerical model is validated in a set of in-house experiments as well as against experimental data available in the literature. The experiment is performed for a limited temperature range (i.e., with a low-density ratio). However, the numerical simulations are carried out by varying the blowing ratio from 0.3 to 0.6. The density ratio during the simulations is kept at 3.5. The minimum distance between the impinging plate and the liner is kept at h/D = 1. A detailed analysis of the numerical results indicates a significant drop in the temperature distribution over the liner surface because of the employed cooling technique. The present study also reveals that, under similar operating conditions, the combined jet impingement and film cooling system has the ability to achieve the targeted cooling effect at a lower bleed air flow rate due to its higher effectiveness than that of the standard film cooling arrangement.
Acoustic Monitoring of Professionally Managed Marine Mammals for Health and Welfare Insights
Authors: Winship Kelley A. , Jones Brittany L.
Published: June 2023

Research evaluating marine mammal welfare and opportunities for advancements in the care of species housed in a professional facility have rapidly increased in the past decade. While topics, such as comfortable housing, adequate social opportunities, stimulating enrichment, and a high standard of medical care, have continued to receive attention from managers and scientists, there is a lack of established acoustic consideration for monitoring the welfare of these animals. Marine mammals rely on sound production and reception for navigation and communication. Regulations governing anthropogenic sound production in our oceans have been put in place by many countries around the world, largely based on the results of research with managed and trained animals, due to the potential negative impacts that unrestricted noise can have on marine mammals. However, there has not been an established best practice for the acoustic welfare monitoring of marine mammals in professional care. By monitoring animal hearing and vocal behavior, a more holistic view of animal welfare can be achieved through the early detection of anthropogenic sound sources, the acoustic behavior of the animals, and even the features of the calls. In this review, the practice of monitoring cetacean acoustic welfare through behavioral hearing tests and auditory evoked potentials (AEPs), passive acoustic monitoring, such as the Welfare Acoustic Monitoring System (WAMS), as well as ideas for using advanced technologies for utilizing vocal biomarkers of health are introduced and reviewed as opportunities for integration into marine mammal welfare plans.
Babesia gibsoni Infection in a Cat with Immune-Mediated Haemolytic Anaemia and Thrombocytopenia
Authors: Almendros Angel , Choi Y. R. , Bęczkowski Paweł M. , Baiker Kerstin , Barrs Vanessa R. , Beatty Julia A.
Published: June 2023

Tick-borne haemoparasite Babesia gibsoni has been detected rarely in cats, in surveys of apparently healthy animals. In stored blood from a 6-year-old male-neutered domestic shorthair cat in Hong Kong, B. gibsoni DNA was detected retrospectively using PCR for Babesia spp. 18S rRNA and mitochondrial cytochrome B genes, followed by sequencing and basic local alignment search tool (BLAST) analysis. The cat presented with severe haemolytic anaemia and thrombocytopenia. The cat responded to supportive care and glucocorticoids and was clinically normal despite persistent subclinical thrombocytopenia until six months after presentation, when it succumbed to a fatal haemorrhagic episode. Necropsy revealed severe intestinal and pulmonary haemorrhage and hypocellular bone marrow with megakaryocytosis but no other causes of immune-mediated thrombocytopenia (IMTP) or immune-mediated haemolytic anaemia (IMHA). Blood stored on days 158 and 180 tested PCR negative for Babesia spp. This report demonstrates that geographic range of B. gibsoni detection in cats includes Hong Kong. The exclusion of other causes suggests that B. gibsoni might have potentially played a role in triggering immune-mediated disease in this case.
Hair Cortisol and Testosterone Concentrations in Relation to Maturity and Breeding Status of Male Feral Horses
Authors: Medill Sarah A. , Janz David M. , McLoughlin Philip D.
Published: June 2023

Steroid-hormone concentrations from non-invasively obtained biomarkers, like hair, can provide a representation of circulating hormones diffused over relatively long time periods (e.g., weeks or months). The hormone cortisol is often associated with physiological or even psychological stress, while testosterone is strongly associated with male development and reproductive success. Increasingly, studies are using hormone levels derived from hair to make inferences among both domestic animals and wildlife. For horses, all previous hair hormone analysis has been done on companion or working animals. We evaluated the levels of hair cortisol (n = 153) and testosterone (n = 48) from 136 feral horses living on Sable Island, Canada that have been part of a long-term individual-based study since 2008. This population has been undisturbed and unmanaged for over 50 years, and exhibits the natural social organization for horses, harem defense polygyny. Hair samples were collected in mid to late summer and the segment analyzed corresponds with hair grown during, and following, the peak of the reproductive season. Social position was determined based on the male’s role as either a dominant breeding Stallion (Stallion), a non-breeding subordinate male (tag), adult Bachelor (5 years old or older), or Immature male (2–4 years of age). While there was no difference in hair-cortisol concentration among any class of adult males (i.e., Stallion, tag, or Bachelor), Immature males had significantly lower hair cortisol concentrations than the other groups (p = 0.001). Hair testosterone levels among the four social positions were significantly higher among Stallions (p = 0.04). Hair testosterone concentration was also significantly related to the probability of a male being either a Bachelor or Stallion and was the only variable remaining in AICc model selection (p = 0.016, AICc = 32.3, Null AICc = 38.8). While not a significant relationship, Stallions had a negative correlation between hair cortisol concentrations and testosterone (R2 = −0.20, p = 0.383), and Bachelors, conversely, had a positive association (R2 = 0.43, p = 0.246). Our observations of hormone concentrations in relation to physiological, social, or reproductive parameters in this population suggest trends that are similar to what has been established using blood or other matrices.
Software- and TDM-Guided Dosing of Meropenem Promises High Rates of Target Attainment in Critically Ill Patients
Authors: Chiriac Ute , Richter Daniel , Frey Otto R. , Röhr Anka C. , Helbig Sophia , Hagel Stefan , Liebchen Uwe , Weigand Markus A. , Brinkmann Alexander
Published: June 2023

Various studies have reported insufficient beta-lactam concentrations in critically ill patients. The optimal dosing strategy for beta-lactams in critically ill patients, particularly in septic patients, is an ongoing matter of discussion. This retrospective study aimed to evaluate the success of software-guided empiric meropenem dosing (CADDy, Calculator to Approximate Drug-Dosing in Dialysis) with subsequent routine meropenem measurements and expert clinical pharmacological interpretations. Adequate therapeutic drug exposure was defined as concentrations of 8–16 mg/L, whereas concentrations of 16–24 mg/L were defined as moderately high and concentrations >24 mg/L as potentially harmful. A total of 91 patients received meropenem as a continuous infusion (229 serum concentrations), of whom 60% achieved 8–16 mg/L, 23% achieved 16–24 mg/L, and 10% achieved unnecessarily high and potentially harmful meropenem concentrations >24 mg/L in the first 48 h using the dosing software. No patient showed concentrations <2 mg/L using the dosing software in the first 48 h. With a subsequent TDM-guided dose adjustment, therapeutic drug exposure was significantly (p ≤ 0.05) enhanced to 70%. No patient had meropenem concentrations >24 mg/L with TDM-guided dose adjustments. The combined use of dosing software and consecutive TDM promised a high rate of adequate therapeutic drug exposures of meropenem in patients with sepsis and septic shock.
An Overview of the Health Benefits, Extraction Methods and Improving the Properties of Pomegranate
Authors: Benedetti Giada , Zabini Federica , Tagliavento Luca , Meneguzzo Francesco , Calderone Vincenzo , Testai Lara
Published: June 2023

Pomegranate (Punica granatum L.) is a polyphenol-rich edible food and medicinal plant of ancient origin, containing flavonols, anthocyanins, and tannins, with ellagitannins as the most abundant polyphenols. In the last decades, its consumption and scientific interest increased, due to its multiple beneficial effects. Pomegranate is a balausta fruit, a large berry surrounded by a thick colored peel composed of exocarp and mesocarp with edible arils inside, from which the pomegranate juice can be produced by pressing. Seeds are used to obtain the seed oil, rich in fatty acids. The non-edible part of the fruit, the peel, although generally disposed as a waste or transformed into compost or biogas, is also used to extract bioactive products. This review summarizes some recent preclinical and clinical studies on pomegranate, which highlight promising beneficial effects in several fields. Although further insight is needed on key aspects, including the limited oral bioavailability and the role of possible active metabolites, the ongoing development of suitable encapsulation and green extraction techniques enabling the valorization of waste pomegranate products point to the great potential of pomegranate and its bioactive constituents as dietary supplements or adjuvants in therapies of cardiovascular and non-cardiovascular diseases.
Evaluation of the Involvement of Heme Oxygenase-1 Expression in Discoid Lupus Erythematosus Lesions
Authors: Fagone Paolo , Piombino Eliana , Mangano Katia , De Pasquale Rocco , Nicoletti Ferdinando , Caltabiano Rosario
Published: June 2023

Discoid lupus erythematosus (DLE) is a chronic autoimmune disease that primarily affects the skin, causing red, scaly patches that may be disfiguring and can cause permanent scarring. This study aimed to investigate the potential clinical and therapeutic applications of heme oxygenase-1 (HMOX1) in the context of DLE. Immunohistochemical staining and bioinformatics analysis were performed on skin biopsy samples from DLE patients to examine the levels of HMOX1 and to correlate with markers of inflammation. Our study revealed a negative correlation between HMOX1 levels and the inflammatory status of DLE lesions, as well as an inverse correlation between HMOX1 levels and the infiltration of M1 macrophages and activated mastocytes. These findings suggest that HMOX1 plays a crucial role in the regulation of inflammation in DLE and could be a potential therapeutic target and biomarker for DLE.
Full-Length Transcriptome Sequencing Reveals the Molecular Mechanism of Metasequoia glyptostroboides Seed Responding to Aging
Authors: Luo Yongjian , Zhang Yixin , Le Jingyu , Li Qing , Mou Jiaolin , Deng Shiming , Li Jitao , Wang Ru , Deng Zhijun , Liu Jun
Published: June 2023

Metasequoia glyptostroboides, Hu and W. C. Cheng, as the only surviving relict species of the Taxodiaceae Metasequoia genus, is a critically endangered and protected species in China. There is a risk of extinction due to the low vigor of M. glyptostroboides seeds, and the physiological mechanism of seed aging in M. glyptostroboides is not yet clear. In order to investigate the physiological and molecular mechanisms underlying the aging process of M. glyptostroboides seeds, we analyzed the antioxidant system and transcriptome at 0, 2, 4, 6, and 8 days after artificial accelerated aging treatment at 40 °C and 100% relative humidity. It was found that the germination percentage of fresh dried M. glyptostroboides seeds was 54 ± 5.29%, and significantly declined to 9.33 ± 1.88% after 6 days of aging, and then gradually decreased until the seed died on day 8. Superoxide dismutase (SOD) activity, ascorbic acid (AsA), glutathione (GSH) content and superoxide anion (O2·−) content and production rate significantly decreased, while malondialdehyde (MDA) and hydrogen peroxide (H2O2) content and glutathione peroxidase (GPX) and catalase (CAT) activity gradually increased during the aging process. A total of 42,189 unigenes were identified in the whole transcriptome, and 40,446 (95.86%) unigenes were annotated in at least one protein database. A total of 15,376 differentially expressed genes (DEGs) were obtained; KEGG enrichment analysis results revealed that seed aging may be mainly involved in the protein-processing pathways in endoplasmic reticulum, oxidative phosphorylation, and ascorbate and aldarate metabolism. Weighted gene co-expression network analysis (WGCNA) revealed that the dark magenta, orange, and medium purple modules were highly correlated with physiological indicators such as SOD, CAT, and GSH and further identified 40 hub genes such as Rboh, ACO, HSF, and CML as playing important roles in the antioxidant network of M. glyptostroboides seeds. These findings provide a broader perspective for studying the regulatory mechanism of seed aging and a large number of potential target genes for the breeding of other endangered gymnosperms.
Experimental Study on the Degradation of Acaricides on the Surface of Kumquat Cuimi by Nonthermal Air Plasma
Authors: Qin Si , Chen Shuo , Wang Xiaonan , Zang Yuanfu , Wang Zifeng , Wei Jie
Published: June 2023

Nonthermal air plasma, which can be generated by air discharge, contains large amounts of reactive oxygen species (ROS), reactive nitrogen species (RNS) and high-energy particles. The ROS and RNS have strong oxidizing properties, and the high-energy particles can break chemical bonds in organic compounds, a process which can be used to degrade organic matter such as pesticides. In the present study, the effects of nonthermal air plasma on the degradation of several pesticides were investigated. An air plasma processing system was designed and constructed to produce nonthermal air plasma and to degrade five commonly used acaricides, namely, avermectin, bifenazate, spirodiclofen, etoxazole, and lufenuron, during the production of kumquat cuimi. The experimental results showed that nonthermal air plasma could degrade all the acaricides on the surface of the kumquat cuimi effectively. After 20 min of plasma treatment, the residues of avermectin, bifenazate, spirodiclofen, etoxazole and lufenuron on the surface of the kumquat cuimi were reduced by 80.67%, 79.52%, 62.40%, 48.93% and 23.11%, respectively. Further analysis indicated that the primary chemical bonds, hydrophobicity, and the pH value of the plasma-activated water can all affect the efficiency of pesticide degradation.
Experimental Study on Mechanical Properties of Rectangular Reinforced Concrete Pipe with Corrosion Defects
Authors: Mei Zhe , Zhang Peng , Zeng Cong
Published: June 2023

Rectangular reinforced concrete pipes are widely used in various municipal projects. Because they are often located in weak strata in urban areas, they are prone to structural defects such as corrosion, which in turn leads to a decline in the bearing capacity of reinforced concrete pipes and, in severe cases, causes disasters such as pipe collapse. In this paper, the mechanical properties of rectangular reinforced concrete pipes with corrosion defects are studied through laboratory tests, and the influence of the pipe failure mechanism and corrosion depth on the ultimate bearing capacity of pipes is analyzed. A numerical simulation method is used to verify the test results, and the numerical simulation results are In good agreement with the indoor test results. The research shows that the failure mode of rectangular reinforced concrete pipe under TEB test conditions can be divided into an uncracked stage, a crack development stage and a failure stage. With the increase of corrosion depth, the ultimate bearing capacity of the pipe decreases. When the corrosion depth is equal to the thickness of the protective layer, the ultimate bearing capacity of the pipe is more than 30%, and the corrosion depth has a great influence on the mechanical properties of the pipe.