bluuebunny/crossref_metadata_2025 huggingface dataset:
Diversity of waterbirds in mudflat and fishpond habitats in coastal Wetlands of East Lampung, Indonesia
Authors: Khalil A R A , Mulyani Y A , Mardiastuti A , Iswandaru D
Published: December 2021

Coastal wetlands provide habitat for waterbirds. However, changes in land use in coastal wetlands in East Lampung, Lampung Province, might affect the use of habitats by waterbirds. The study objective was to identify waterbird species using wetland habitat in coastal areas of East Lampung. Field surveys were conducted in November 2020 in two habitat types, i.e., mudflats and fishponds, employing a concentration method from several vantage points, then calculating Shannon-Wienner diversity indices (H’). A total of 23 species from 9 families of waterbirds were recorded. Mudflat had a higher diversity (H’½2.21) than fishpond (H’=1.74). Sixteen species were found on mudflats, dominated by shorebirds from the families of Scolopacidae and Charadriidae, which are primarily migratory species. Fishponds were used by 12 species, dominated by the Little Egret (Egretta garzetta) and Glossy Ibis (Plegadis falcinellus). Two endangered species, i.e., Far Eastern Curlew (Numenius madagascariensis) and Milky Stork (Mycteria cinerea), and one vulnerable species, Lesser Adjutant (Leptoptilos javanicus), were observed using mudflats. This study showed that mudflat in coastal wetlands of East Lampung provides essential habitats for conserving threatened waterbirds and migratory shorebirds.
An optimized method for high-quality DNA extraction medicinal fungi Mycoleptodonoides aitchisonii for whole genome sequencing
Authors: Riffiani R , Wada T , Shimomura N , Yamaguchi T , Aimi T
Published: December 2021

The extraction of pure high molecular weight DNA and collecting genomic DNA from fungi is difficult. This is because filamentous fungi has a sturdy cell wall, high protein and polysaccharide levels resistant to the usual DNA extraction procedures. A low-cost and reliable DNA extraction method was designed from Mycoleptodonoides. aitchisonii fit for whole-genome sequencing for identification and mapping of the A mating-type gene. Mycoleptodonoides. aitchisonii belongs to the Climacodontaceae family has pharmaceutical activities. In the present study, the mycelia of M. aitchisonii, which grew from the different concentrations of malt extract and minimal liquid media, have been compared systematically to determine the DNA extraction procedure resulted in DNA concentration with excellent purity and quality. The best protocol that resulted in good quality DNA was further validated using polymerase chain reaction amplification with a specific primer to amplify the homeodomain protein (Mahd2-18) gene that encodes a transcription factor. The method proposed DNA extraction using CTAB (Cetyl Trimethyl Ammonium Bromide) method and purify by commercial kit from mycelium grown in the minimal liquid media give the best result with the high concentration of DNA for whole-genome sequencing by Next Generation Sequencing and other applications.
Callus regeneration and polyploidy induction of Allium cepa L var. Bima Brebes using oryzalin
Authors: yun R Q A’ , Dinarti D , Husni A , Kosmiatin M
Published: December 2021

Polyploidy induction could increase shallot bulb-size to raise consumer preference and local shallot productivity. The research aimed to obtain an effective method of polyploidy induction on callus of onion (Allium cepa) var. Bima Brebes. The experiment was consisted of two experimental steps, which were callus induction of onion and polyploid induction of the callus. A 1×1 cm callus was treated by two drops of oryzalin with concentrations 0, 25, 50, 75, 100, and 120 μM. The ploidy level was identified based on morphological trait, stomatal analysis and DNA content using a flow cytometry. The results showed callus diameter, number of green spots, and number of shoots were decreased with increasing oryzalin concentration. The planlet leaves regenerated from oryzalin treated callus were darker than that of control. The flow cytometry analysis showed that planlets with 75 μM oryzalin was tetraploid, had longer and wider stomata than that of the control.
Biology and life table of Trichogramma chilotraeae, Egg parasitoids of Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae)
Authors: Sari L , Maryana N , Hidayat P
Published: December 2021

Spodoptera frugiperda is a species of armyworm that attacks maize crops globally and is a dangerous new invasive pest in Indonesia. Biological control, which employs natural enemies, is one method of controlling this pest. One of the natural enemies found in the field is the egg parasitoid of the Trichogrammatidae family. This study aimed to determine the reproductive capacity of the parasitoid on S. frugiperda eggs. The tested parasitoids were collected from S. frugiperda eggs on corn cultivations in Tenjolaya Village, Bogor Regency, West Java Province. The identification results showed that the parasitoid species used in this study was Trichogramma chilotraeae. The development periods of eggs, first instar larvae, second instar larvae, and pupae in the laboratory were 1.16 days, 1.18 days, 1.39 days, and 3.28 days, respectively. The reproductive capacity of T. chilotraeae on S. frugiperda eggs, referred to as the net reproduction rate (Ro), was 28.13, gross reproduction rate (GRR) was 30.43. the average time for one generation (T) was 9.07. The intrinsic rate of increase (r) was 5.54, and Doubling time (DT) was 1.88. Thus, the egg parasitoid of T. chilotraeae was not very effective as a potential biological agent for S. frugiperda.
Macrofungal identification and species richness in Cigelung Landscape and its influence on the presence of oil palm pathogens in PTPN VIII
Authors: Arko P F , Sudirman L I , Qayim I
Published: December 2021

Dungus Iwul Nature Reserved (CADI) is a remnant patch of tropical rainforest converted into plantations with neither past data nor study in macrofungi. In this article, we explored and identified macrofungal fruitbody in CADI and PTPN VIII Oil Palm Plantation (PTPN) around the nature reserve. The inventory was carried out with the opportunistic sampling methods assisted by the line intercept. Morphological characteristics were used to identify the macrofungal fruitbodies species. We found that the species richness in the study area stood at 120, with 70 species found in CADI, 23 species in PTPN planted in 2003, and 57 species in PTPN planted in 2004. These 120 species consist of 76 genera, 41 families, 11 orders, and four classes in Subkingdom Dikarya. Schizophyllum commune and Marasmiellus candidus in CADI and S. commune in PTPN planted in 2003 and 2004 were the species found with the highest relative frequency. Neither sign of basal stem rot on oil palm trees nor Ganoderma fruitbodies were found in both PTPN study locations, even though the fruitbodies were found in CADI. Further research is needed to determine if nature reserve could be a barrier against pathogens of monoculture oil palm plantation in a similar landscape model.
Bird visit to Ficus benjamina in two urbanization gradients in the tropics
Authors: Mardiastuti A , Mulyani Y A , Kusrini M D
Published: December 2021

Fig trees area believed to be important bird habitat in the tropics, including in urban areas. The research objective was to reveal the bird species that visited Ficus benjamina in the low and high urbanization gradients in the tropics. Data were obtained in IPB University Darmaga Campus (low urbanization) and Sentul City (high urbanization) in Bogor (West Java, Indonesia), through direct observations of four trees per site in the morning, midday, late afternoon, and night, totalling 276 observation hours. Total of 29 bird species visited F. benjamina trees (26 species in low urbanization, 12 species in high), mainly insectivores, nectarivores, and frugivores birds. Nine species were common in both sites, i.e., Spotted Dove, Plaintive Cuckoo, Fulvous-breasted Woodpecker, Small Minivet, Common Iora, Sooty-headed Bulbul, Yellow-vented Bulbul, Common Tailorbird, Scarlet-headed Flowerpecker, and Olive-backed Sunbirds. Nocturnal birds (Collared Scops Owl, Large-tailed Nightjar) were present in low urbanization, but absent in high urbanization site, so did kingfishers (White-throated Kingfisher, Collared Kingfisher) and some other urbanization-prone species. The high urbanization site was characterized by the presence of Eurasian Tree Sparrow at the fig tree. This study showed that F. benjamina has an important role for diurnal and nocturnal birds, even in the high urbanization site.
Mechanical properties of edible film based bacterial cellulose from sago liquid waste using starch as stabilizer
Authors: Yanti N A , Ahmad S W , Ramadhan L O A N , Walhidayah T
Published: December 2021

Edible film is a thin layer made of edible material as a packaging for food products. In the edible film production, required additional material that serves as stabilizer and plasticizer. This research aimed to determine the mechanical properties of the edible film-based bacterial cellulose from sago liquid waste using some types of starch as a stabilizer. The starches used as stabilizers in making edible films were corn, cassava, and sago starch. Mechanical properties were measured include tensile strength, elongation at break, elasticity (Young’s modulus), and water holding capacity (WHC). The results showed that the thickness of corn, sago, and cassava starch was 0.08 mm, 0.09 mm, and 0.11 mm, respectively. The mechanical properties of the edible film with corn, sago, and cassava starch as stabilizers namely a tensile strength (MPa) were 10.90; 15.90 and 61.92 respectively, elongation at break (%) were 8, 20, and 87, young’s modulus (MPa) were 13.48, 7.84 and 6.98, respectively and water holding capacity (g/g) were 34.26; 18.18 and 16.40 respectively. Therefore, the utilization of starch as a stabilizer in edible film can improve its mechanical properties.
Chitosan-Ag nanoparticle antifungal activity against Fusarium sp., causal agent of wilt disease on chili
Authors: Wahyuni S , Wibowo K R P , Prakoso H T , Bintang M , Siswanto
Published: December 2021

Chitosan is a polysaccharides compound derived from nature and has an amine group and a hydroxyl group that gives it unique physicochemical properties, which are polychationic and chelating agent. This shows that chitosan can be used as a stabilizer and reducer of Ag nanoparticles. Unlike other studies, chitosan that was used in this study was derived from Black Soldier Fly exuviae which has been through demineralization, deproteination, depigmentation, and deacetylation processes. This study aims to obtain an alternative environmentally friendly fungicide with Ag-chitosan nanoparticle formulation which has antifungal activity against Fusarium sp. that causes wilt disease in chili plants. We analysed particle size, functional group by FTIR, and antifungal test against Fusarium sp. on 1000, 750, 500, 250, and 100 ppm of chitosan-Ag nanoparticle. The chitosan-Ag nanoparticles have a particle size of 188.4 nm with a polydispersity index of 0.344. FTIR analysis showed that Ag+ ions are successfully grafted into the chitosan matrix. All the chitosan-Ag nanoparticle concentration tested showed significant results with negative and positive control, except the concentration of 100 ppm which is not significantly differ from negative control. The antifungal test showed a concentration of 750 ppm chitosan-Ag nanoparticles had a growth inhibition of 72.17%.
Isolation, characterization, and screening of antioxidant activity of endophytic bacteria from Leea Indica (Burm. F) Merr. Leaf
Authors: Arivo D , Mubarik N R , Rusmana I , Batubara I
Published: December 2021

Endophytic bacteria are bacteria that live within plant tissues and can produce the same compounds as their host. Leea indica, commonly known as bandicoot berry, is a medicinal plant with various bioactive compounds. A study on the diversity and antioxidant activity of endophytic bacteria from L. indica has never been reported. So this study is essential to conduct to explore the diversity and antioxidant activity of endophytic bacteria in the L.indica plant. The objective of the study was to isolate, identify, and screening of antioxidant activity of endophytic bacteria from L. indica leaf. We obtained 51 bacterial isolates with various of morphology. Microscopic identification showed that 44 isolates were categorized into Gram-negative (23 rod-shaped, 18 cocci-shaped, and 3 cocci bacilli-shaped isolates), and the other 7 isolates were categorized into Gram-positive (6 rod-shaped and 1 cocci-shaped isolates). The biochemical test showed that 100% of isolates have positive catalase, and 13.7% were suspected of having pathogenic activity on blood agar. The antioxidant activity showed that 17 isolates (more than 70%) had high inhibition by scavenging free radicals. It can be concluded that there are various endophytic bacteria isolated from L. Indica leaf and potentially producing antioxidant activity as their host.
Antibacterial activity of endosymbiotic fungi isolated from marine sponges collected from Kotok Kecil Island, Seribu Islands, Jakarta
Authors: Trifani R , Noverita  , Hadi T A , Sinaga E
Published: December 2021

Endosymbiont fungi from marine sponges are a rich source of medicinally active compounds. Indonesia has a huge number of marine sponges. This research was conducted to determine the antibacterial potential of endosymbiont fungi isolated from marine sponges collected from Kotok Kecil Island, Seribu Islands. The screening was conducted with two methods, the modified GIBEX and disc diffusion. Species of marine sponges were isolated, namelyPetrosiasp.,Stylissa carteri, Cinachyrella australiensis, Callyspongiasp.,Petrosia nigrians, andStylissa massa, and obtained 9 isolates of endosymbiont fungi. The GIBEX test againstEscherichia coliandStreptococcus mutansshowed that ethyl acetate extract had strongest antibacterial activity. Disc diffusion test of ethyl acetate extract, five isolates had antibacterial activity againstS. mutansand four againstE. coli. The isolate that showed the strongest antibacterial activity was fromPetrosiasp. Which has an inhibition zone of 8.4 mm againstE. coliand 7.45 mm againstS. mutans. The main active compounds from the isolate ofPetrosiasp. are butylhydroxytoluene and phthalic acid di-(2-propylpentyl) esters. Based on this study, we concluded that the endosymbiont fungi of marine sponges are potential to be developed for further development as source of antibacterial agents.
Analysis of climatic variability and dengue hemorrhagic fever incidence at the Tsunami Area Banda Aceh City
Authors: Agustina E , Leksono A S , Gama Z P , Yanuwiadi B
Published: December 2021

The rebuilding of post-tsunami community residential an area in Banda Aceh City has created a new ecosystem and at the same time a public health problem. The construction of new settlements has an impact on the emergence of Aedes aegypti habitat. The post-tsunami climate factor is thought to be one of the factors that cause outbreaks of dengue hemorrhagic fever (DHF) cases every year in Banda Aceh City. This study aims to analyze the relationship between climatic variability with the DHF incidence cases from 2010-2020 at the tsunami area Banda Aceh City. The study uses monthly DHF case data obtained from the Banda Aceh City Health Office. Monthly climate data were obtained from the Meteorology, Climatology, and Geophysics Agency of Aceh Province. Spearman’s correlation analysis shows that temperature, humidity, rainfall, and wind velocity have a significant relationship with the DHF incidence cases. Humidity and rainfall are positively correlated with DHF incidence, while temperature and wind velocity shows a negative correlation with DHF incidence. This study shows that climate is one of the factors influential in the DHF incidence cases at the tsunami area Banda Aceh City.
Development and characterization of Simple Sequence Repeats (SSRs) markers in durian kura-kura (Durio testudinarius Becc.) using NGS data
Authors: Magandhi Mahat , Sobir  , Kusumo Yudiwanti W.E. , Sudarmono  , Matra Deden Derajat
Published: December 2021

Durian Kura-kura (Durio testudinarius Becc.) belongs to the Malvaceae family and is an endemic species of Borneo. Recently, genomic-based next-generation sequencing (NGS) approaches have been carried out for germplasm conservation and plant breeding programs. The NGS technologies allow plant genomes to be sequenced quickly and inexpensively and enable the efficient development of SSR markers through the in-silico approaches. This study aimed to develop and characterize simple sequence repeats (SSRs) from the assembled genome. The 1203929 scaffolds of the assembled genome were produced from the Ray assembler. The SSRs were identified and extracted using the MISA program produced 4315 sequences containing SSRs. The six motif repeats of SSRs were identified; consist of 431 sequences of dinucleotide (the most motif is AT), 3257 sequences of trinucleotide (the most motif is TTA), 516 sequences of tetranucleotide (the most motif is AAAT), 89 sequences of pentanucleotide (the most motif is ATTTT), 18 sequences of hexanucleotide and four sequences of heptanucleotide. The new SSRs markers will be used in further studies of genetic population of D. testudinarius and plant breeding programs.
Prospects of neem and citronella oil against Pseudococcus longispinus (hemiptera: Pseudococcidae) on Phalaenopsis
Authors: Hutapea D , Rahardjoa I B , Thamrin M
Published: December 2021

Synthetic insecticides are still widely used in gardens and greenhouses of ornamental crops to reduce populations of insect pests and preserve flower quality. The improper and unwise use of synthetic insecticides has caused various negative impacts on humans, insect pests, and the environment. Therefore, the quest for unharmed insecticides and environmentally friendly is being sought. The purpose of this study was to examine the effectiveness of botanical insecticide formulation of neem and citronella oil to control long-tailed mealybug Pseudococcus longispinus. Four insecticide concentrations of neem, citronella oil and its mixtures (0.5, 1, 1.5, 2 per cent) were tested for their effectiveness by adding each with Latron at 0.5% as an emulsifier. Bioassays were performed in the laboratory by spraying insecticides on Phalaenopsis leaves infested with P. longispinus groups of one size class, mostly represented by the third instar nymphs. The results revealed that all insecticides tested caused mortality after 24 hours. The combination of neem and citronella in 1.5% provided adequate protection by reducing P. longispinus populations by up to 86%. Thus, botanical insecticides can be recommended as a viable alternative to synthetic insecticides against P. longispinus which infested orchids.
Implementation Of Road Asset Management In Poland
Authors: Zofka Professor Adam , Maciejewski Andrzej
Published: December 2021

Road asset management (RAM) is a crucial aspect in the development of transportation network. RAM framework should be understood as a rational approach to business model for road authority, which dictates its business processes in a systematic and objective-based manner to ensure that strategic goals of an agency are reached. In a case of road network, such objectives are typically related to travel safety, time and comfort that should be maintained throughout the life cycle of the road assets at optimal costs. As such RAM enables road agencies to meet expectations of its customers to provide safe and reliable road network in effective and efficient way. Nowadays it becomes even more important to adopt performance-based and data-driven approach for road agencies considering current trends in transportation like connected, cooperative and automated mobility.

There are many aspects of RAM including legal, economic, technical and managing aspects. These aspects are inter-connected and nowadays agencies build and incorporate RAM system to establish a common denominator for all agency activities at all organizational levels. This paper discusses selected aspects of RAM in Poland. Since national road network in Poland is relatively new, it is particularly important to go over well designed and detailed implementation process. It should comprise numerous items including self-assessment and gap analysis, change strategy and transportation asset management plan (TAMP). This paper present update on these activities as well as presents future plans in order to establish modern, effective and sustainable RAM framework in Poland.
Planning Of State Road Renewal In The Context Of Territorial Reform
Authors: Skabs Salvis
Published: December 2021

In 2021 a new territorial reform will be carried out in Latvia, and the number of existing 110 local governments will be reduced to 42 regions (previous territorial reform in 2009 resulted in the decrease of the number of local governments from 522 parishes down to 110 local governments).

Each territorial reform has always regarded the importance of the network of state owned roads in ensuring successful operation of local governments, management of their territories and provision of municipal services to local inhabitants.

After the previous territorial reform carried out in 2009 there were 104 centres of local governments that were not connected with paved roads. At present only 59 such centres without paved road connections have remained, and improvements on roads serving these local governments is continued in the scope of annual improvement programmes financed from the state budget.

The Ministry of Environment and Regional Development within the scope of the new territorial reform in 2021 is preparing a new Investment Programme for Road Development in the Context of Territorial Reform. Respective road sections are identified by the administrations of planning regions in co-operation with local governments, the programme itself is compiled by the Ministry of Environment and Regional Development, but State Limited Liability Company “Latvian State Roads” is providing consulting on the choice of most efficient rehabilitation methods, preparation of technical documentation and potential construction costs.
Viastructura – A New Way Of Pavement Structure Design
Authors: Vaitkus Audrius , Kleizienė Rita , Karbočius Martynas
Published: December 2021

The catalogues of standard pavement structures are common way to design road pavements. In 2019, new regulation for the design of standard pavement structures KPT SDK 19 was issued in Lithuania. One of the new requirements require verification of layer thickness of high-class pavement structures. Such verification should be done by internationally approved mechanistic-empirical methods. In addition, it is recommended to use the same methods to adjust the layer thickness of the selected standard pavement structure for lower classes. These calculations are particularly applicable when the design load (ESAL) is at the lower or upper limit of the class range. Vilnius Tech Road Research Institute experts and outsource IT specialists spent two years for the design model ViaStructura development. Web software based on mechanistic-empiric approach include the boundary conditions, based on Austria, the United States and Germany experience and laboratory test results of construction materials. Materials can be selected from created database, which can be simply expanded with the new materials by the user. as Additional function allow comparison of separate designed pavement structures. The article present the concept of the ViaStructura model for the design of flexible pavement structures, reveals its main principles and advantages comparing to the pavement structure selection by the standard catalogue.
Road Design And Construction On Low Bearing Capacity Soils Using Piling Method: Experience Of SLLC “Latvian State Roads”
Authors: Ābols Mārtiņš , Dārznieks Ojārs , Spilva Māris
Published: December 2021

The road system in Latvia has evolved over time to a complex network of physical structures that include roads, bridges and overpasses, however, in large it consists of narrow roads of local significance that follow the existing topography and consist of thin foundation and pavement layers. In-depth geotechnical research of road sections before construction has only been carried out in recent decades, thereby with a certain regularity SLLC “Latvian State Roads” has to deal with low bearing capacity soils under the road foundation. As the recent experience shows, there are sections of roads that in the past were constructed on peat. In 2018 during the reconstruction works of the regional road P32 Augšlīgatne – Skrīveri a low bearing capacity soil under the road foundation was determined. An additional in-depth geotechnical research showed a bog section with a peat layer at a depth of 10m in a 320 metres long section. Although at that point it was possible to continue the work using simple soil stabilization methods, there were concerns about the longevity of the investment. To stabilize the road foundation, a combination of gravel columns and unreinforced concrete pile columns was used. A total of 952 columns were constructed. The aim of this paper is to share technical information and our good practice of road design and construction on low bearing capacity soils using piling method, and it gives a summary of field observations, geotechnical research, design considerations and risk management assessments that were carried out in this specific case. The positive outcome of this case led to a number of future projects where similar methods for load bearing capacity improvement were planned and used.
Falling Weight Deflectometre And Plate Load Test: Direct And Indirect Comparative Testing
Authors: Taranovs Andrejs
Published: December 2021

Plate load test is a widely used method in Latvia both in quality control and in road design process. This test is performed according to the standard DIN 18134. Such test usually takes at least 30 minutes and requires certain load weight. Considering the relatively long time needed for this test, alternatives were sought and a potential alternative was defined to perform testing with Falling Weight Deflectometre (FWD). In order to check this assumption both direct and indirect testing was performed and correlation between the results of both tests was defined.

In the first case the test was performed in the same location with both pieces of equipment on a surface of unbound pavement. In the second case the test with Falling Weight Deflectometre was performed on the surface of bituminous pavement but plate load test was performed in the same location on the surface of base course with prior demolition of bituminous layers. In order to compare the results of indirect comparative testing, the backcalculation for the data acquired with Falling Weight Deflectometre was performed according to German calculation method.

Results acquired with direct testing showed that the testing with Falling Weight Deflectometre and plate load test are interchangeable if no characterization of the layer compaction is required. The German method of backcalculation (FGSV, 2014) is very simple. Despite positive references from other specialists this method in comparative testing did not show sufficiently good correlation with the results acquired in plate load test.
Adaptation And Resilience From A Maintenance Perspective For Swing Bridge. Lessons Learned In Recent Retrofitting Project Experiences
Authors: Collazos-Arias Felipe , García-Sánchez David
Published: December 2021

This paper collects all the existing swing bridges around the world to emphasize the high-level technology performed in the earliest 20th century and analyzes the recent experience in the rehabilitation and retrofitting of a singular swing steel bridge.

The bridge over the Asón estuary at Treto, in the North of Spain, showed significant problems and the structural condition level was critical previously to its intervention in 2015. The rehabilitation project including the restoration of the structural and functional safety level of the structure was deled after material studies and tests were performed. The new updated of the structure from the old nineteenth centuries to the new standard codes was also performed. A part from the historical value of the bridges and the "green" and safe-security aspects, the intervention took into account all the social requirements of the population in the area who recovered the pride in this emblematic and centenarian infrastructure and it is, itself, a successful intervention from the resilient point of view.
High Performance Pavement Markings Enhancing Camera And LiDAR Detection
Authors: Sauter Gernot , Doring Marcel , Nuyttens Rik
Published: December 2021

It is well known that camera and video sensors have limitations in detecting pavement markings under certain conditions e.g. glare from sunlight or other vehicles, rain, fog etc. First generations of lane keeping systems depend on visual light. Erroneous detection is also resulting from irregular road surfaces such as glossy bitumen sealing strips, rain puddles or simply worn asphalt. The role of higher performing markings and better visual camera detection has been studied with Vedecom France. LiDAR (light detection and ranging) technology could help to fill remaining gaps, as it actively sends out IR (infrared) light, that returns reliable images of the road scenario and pavement markings both day and nighttime. In order to evaluate the opportunities of LiDAR technology for the detection of road markings, 3M Company and the University of Applied Sciences in Dresden decided to work together in a joint research project. All-Weather Elements AWE, are the latest development of high-performance optics, using high index beads to provide reflectivity both in dry and wet condition. It could be determined that high performance markings help to increase the level of detection by both camera and LiDAR sensors. The AWE marking was detected from significantly longer distances, especially in wet and rainy conditions. In combination with common camera based LKA and LDW systems, the LiDAR sensors can increase the overall detection rate of pavement markings. This is especially important for vehicles with higher SAE levels of automated driving and can support the overall safety of vehicles. The research also evaluated existing test methods for wet and rain reflectivity in EN 1436 and ASTM E 2832 and how measured performance correlates with LiDAR detection.
The Rolling Out Of International Project SMART E263/E77 For Advanced Traffic Management
Authors: Jelisejevs Boriss , Duubas Kristjan
Published: December 2021

Intelligent transportation systems (ITS) provide significant added value to road transportation, making the related investments distinctively effective and long-lasting. Moreover, some ITS activities may be eligible for financial support of the European union (EU). That was the way how Estonian Transport Administration and Latvian State Roads worked on the project proposal “Smart corridor Tallinn-Tartu-Luhamaa-Riga E263/E77” (acronym – SMART E263/E77), which was approved by EU program Interreg Central Baltics as CB891 project. The project started on June 1, 2020, and its implementation will last till the end of 2022 according to quite challenging schedule. Project activities primarily include numerous installations or road telemetry and telematics devices (especially, variable message signs) for advanced traffic management to be supported by cross-border traffic plans and improvements of traffic control centers. Project target is to provide general travel time savings at least by 0.88% across the whole corridor, however for the motorway-type sections it should reach more than 5.5%. Expected project results will establish new and improve existing functions on the E263 and E77 road transport corridors, namely: traffic management adaptive to variable road conditions; gathering and dissemination of traffic information; decision-making support for road maintenance operations (especially in winter). This report will summarize the information on project progress with emphasis on traffic management considerations.
Construction Of Economical Pavement Structures With Wood Ash
Authors: Dimter Sanja , Zagvozda Martina , Tonc Tea , Šimun Miroslav
Published: December 2021

Stabilized mixes that are used in pavement structures are composed of aggregate bound with hydraulic binders (cement, lime) or bitumen. The most commonly used for the construction of base layers are mixes stabilized with cement. A long-standing construction practice for pavement structures was based on the use of quality granular materials for the construction of base layers. However, when designing the pavement structure and selecting materials, economy, sustainability, and environmental impact, in addition to their mechanical properties, should also be considered. Clear requirements and guidelines for sustainable development have imposed the need to explore the possibility of using non-standard materials in construction. Wood ash, which is formed as a residue from the combustion of biomass in the production of electricity and heat, is one of the newer and, in Croatia, less researched alternative materials that can be applied in construction. The paper describes compressive strength tests of mixtures of sand from the Drava River and cyclone wood ash stabilized with various contents of cement. The obtained results showed that with wood fly ash (in a content of 30 % mass.) in the stabilization mixture of sand, values of compressive strengths can be achieved within the required limits necessary for the construction of base layers of the pavement structure stabilized by a hydraulic binder.
A comparative study of structural, mechanical & electrical properties of ZnO and AlN thin films for MEMS based piezoelectric sensors
Authors: Srivastava Vinay Kumar , Singh Jaspreet , Kumar Parlad , Arora Sataypal Singh , Singh Satinder Pal , Singh Surinder
Published: December 2021

In this work, a comparative study has been carried out to compare the relative performance of ZnO (Zinc Oxide) and AlN (Aluminum Nitride) thin films for their application in piezoelectric sensors. The thin films material properties are being characterized using various material characterization techniques such as SEM, XRD, and Nanoindentation. Further the MIM (Metal-Insulator-Metal) based devices have been fabricated with piezoelectric films sandwiched between Al electrodes. The devices have been evaluated for mechanical and electrical performances. The natural frequency of the devices recorded as 46.8 kHz (ZnO) and 40.8 kHz (AlN). The average nominal capacitance of the MIM structure is measured as ∼98 pF and ∼120 pF where as corresponding dissipation factor obtained as ∼0.03 and ∼0.0005 respectively for ZnO and AlN devices. The repeatability investigation carried out on the sample devices for up to 90 days and the output has been monitored. The result showed that the AlN devices exhibit better output stability compared to ZnO devices.
Effect of multiple forging on the microstructure and properties of an as-cast Cu–Ni–Si alloy with high Ni and Si contents
Authors: Zhang Jinlong , Lu Zhenlin , Jia Lei , Xie Hui , Wei Xin , Tao Shiping
Published: December 2021

A Cu–Ni–Si alloy with high Ni and Si contents was prepared by the traditional melting and casting method, and then multiple forging and ageing were conducted to investigate their effect on the microstructure and properties. The results show that reticular Ni31Si12 phases are located on the grain boundaries of the dendritic α-Cu(Ni, Si) solution matrix in the as-cast Cu–Ni–Si alloy because of the high Ni and Si contents, and some rice-like Ni2Si phases precipitate in the interior of α-Cu(Ni, Si) grains during cooling. With increasing number of forging passes, the morphology of the α-Cu(Ni, Si) matrix changes from dendrites to elongated dendrites and then equiaxed grains, the Ni31Si12 phase changes from reticular to irregular and then particle-like, while the Ni2Si phase gradually disappears. As a result, the hardness increases continuously up to 18 forging passes, while the electrical conductivity first increases and then decreases significantly. The hardness and electrical conductivity achieve the highest values with 18 forging passes and a subsequent ageing treatment at 450 °C for 4 h, and the corresponding microstructure comprises an equiaxed α-Cu(Ni, Si) matrix with microscale Ni31Si12 particles and sub-microscale Ni2Si precipitates.
V-OG01 Minimally invasive oesophagectomy: Technique of linear stapled anastomosis
Authors: Knight Benjamin
Published: December 2021

Background

Several anastomotic techniques have been described when performing an oesophagectomy. Each technique has its own merits and drawbacks. The stapled side to side technique creates a widely patent anastomosis with low stricture rate.

Methods

This video highlights the technique adopted and developed over the last 5 years. There are several key steps that need to be adhered to, to create a reliable, robust and reproducible anastomosis. These include the orientation of the oesophagus during transection, the use of mucosal retaining sutures, the use of a 34 bougie for the oesophagotomy and the correct retraction of the conduit when performing the anastomosis.

Results

The anastomosis was successfully performed without complications. Check endoscopy revealed a widely patent secure join. The anastomosis typically now takes 15–18 minutes. At the end of the procedure, the conduit cap was buried under the pleura and the anastomosis wrapped in omental fat. The patient was discharged on day 10 on a low residue diet.

Conclusions

This technique has been adopted and developed over the last 5 years. It has proved reliable and reproducible with a low stricture rate and a very low leak rate. It is easier to perform than a total hand sewn anastomosis and permits visualisation of the luminal oesophagus prior to anastomosis.
V-OG02 Video Demonstration Of Abdominal Lymphadenectomy In A Robotically Assisted Oesophagectomy
Authors: Chmelo Jakub , Brown Joshua , Prasad Pooja , Hayes Nick , Navidi Maziar , Phillips Alexander , Wahed Shajahan , Immanuel Arul
Published: December 2021

Background

Lymphadenectomy is essential for adequate oncological clearance and accurate staging during oesophagectomy for malignant disease. Adequate lymph node clearance has implications on patient outcomes and confers a survival benefit. Abdominal lymph node clearance may be technically challenging due to the location of nodes along key structures such as the common hepatic artery and splenic vessels. Robotic assistance during abdominal lymphadenectomy permits improved 3-D visualisation and instrument articulation in a potentially constricted space. This video demonstrates a technique for robotic abdominal lymphadenectomy during oesophagectomy.

Methods

This video demonstrates a technique for coeliac axis lymph node clearance during the abdominal phase of an oesophagectomy, as practiced at this institution. The intention for such a video is for ongoing appraisal and refinement of robotic techniques within the unit, as well as for teaching and training. The video was edited using iOS software, and text has been used to explain each step in conjunction with the images.

Results

Dissection of all relevant coeliac axis nodal stations is successfully demonstrated, with the lymph nodes resected en-bloc with the specimen. Text has also been used to explain the steps seen in the video images.

Conclusions

Robotic assistance permits safe and adequate lymphadenectomy during minimally invasive oesophagectomy, as demonstrated in this video.
O-B02 Robotic versus Laparoscopic Roux-en-Y Gastric Bypass: A comparison of short-term outcomes and weight loss
Authors: Hamdan Mohammed , Messina Gianfranco , Duck Eleanor , Shenoy Aniruddh , Gill Gurpreet Singh , Jones Gregory , Sampson Marianne , Ramus James
Published: December 2021

Background

The benefits of robotic over laparoscopic surgery for Roux-en-Y gastric bypass (RYGB) are debatable, with current evidence suggesting no significant differences in short-term outcomes. This study compares short-term outcomes and excess weight loss (EWL) % difference between these two techniques.

Methods

A retrospective study of patients undergoing RYGB between January 2016 and November 2020 at a single centre. Demographic, peri-operative and EWL% data were analysed.

Results

424 RYGB procedures were performed by three surgeons including 77 robotic (RRYGB) and 347 laparoscopic (LRYGB) operations. The first 8 RRYGB were excluded being early in the learning curve and the operative technique was modified afterwards. There were no statistically significant demographic differences. The median operative time was 179 (151 – 195) and 149 (123 -171) minutes in the RRYGB and LRYGB groups respectively (P < 0.001). There were no statistically significant differences between both groups in complications, length of stay, 30-day readmission and EWL% at 6 and 12 months. The EWL% at 2 years was 88.5 (+/-19.1) and 66.6 (+/-29.8) in the RRYGB and LRYGB groups respectively (P = 0.003).

Conclusions

RRYGB increases the operative time with no significant short-term outcome differences. The EWL% was higher at 2 years, probably due to a narrower hand-sewn gastro-jejunal anastomosis.
O-BN03 Laparoscopic Common Bile Duct Exploration – Delivered within UK NHS Healthcare Framework
Authors: Kostalas Marcos , Christopoulos Petros , Platt Tim , Sinha Surajit , Bowling Kirk , Srinivas Gandrapu , Andrews Stuart
Published: December 2021

Background

Cholelithiasis is a common problem in the UK affecting approximately 15% of the population. The incidence of synchronous choledocholithiasis is approximately 10-18%. The approach to bile duct stones is variable. Single stage bile duct exploration and cholecystectomy (LC) vs two stage ERCP followed by LC has been shown to be equally safe and as effective with reduced length of stay and number of procedures. We describe the results of a single, high volume centre performing laparoscopic common bile duct exploration (LCBDE) as an alternative to ERCP.

Methods

All patients undergoing LCBDE at our institution from November 2013 – July 2021 were included in the study. Data were collected from a prospectively maintained institutional database and data points corroborated by electronic patient data on hospital systems.

Results

304 patients underwent LCBDE. Median age was 68 (range 21-94). Most cases were performed as urgent/emergency (n = 204, 67% vs n = 100, 33% elective). Bile duct stones were diagnosed pre-operatively in 32.8% cases (n = 100). Intra-operative diagnosis was made using laparoscopic ultrasound (n = 221, 73%), cholangiogram (n = 44, 15%) or combination of both (n = 31, 10%). Laparoscopic completion rate was 92%. Successful stone clearance rate was 98%. 56% were via choledochotomy and 44% trans-cystic. Incidence of bile leak was 4.9% (n = 15). Median length of stay was 2 days post-operatively (range 0–62). The rate of all complications was 13.2%. The rate of mortality was 0.66%.

Conclusions

This is the largest single case series of LCBDE published. This study has demonstrated that a safe and effective LCBDE service can be delivered within the NHS, with outcome data comparable to defined performance standards. With the evolution of specialist training, intra-operative imaging +/- LCBDE is likely to be the preferred modality of treatment.
O-BN09 The impact of the Covid-19 pandemic on benign upper GI surgery: The first 12 months at a single centre
Authors: Michel Martin , Fifer Helen , Moran Emily , Hammett Felix , Bonner Clare , Kronberga Madara , Khawgali Mohamed , Balbola Mohamed , Saha Arin
Published: December 2021

Background

The road to recovery from the Covid-19 pandemic has started but no-one knows when it will end. 18 months on from the World Health Organisation declaring a global pandemic on the 11th March 2020 this has had a dramatic impact on both acute and elective hospital services. Whilst, quite rightly, the focus has been on prioritising cancer resections during the pandemic, many patients awaiting benign operations are facing lengthy waiting times. The aim of this study was to quantify the impact of the COVID-19 pandemic on benign upper GI surgery at a single centre compared to previous operating activity levels.

Methods

Retrospective analysis of computerised theatre records for the first 12 months of the pandemic (11th March 2020-11th March 2021) were compared to average historical data (HD) over the last five years (2015-2019) over the same time frame. Benign upper Gi operations included were cholecystectomy, anti-reflux/hiatus hernia repairs, cardiomyotomies and bariatric procedures.

Results

Conclusions

The Covid-19 pandemic has dramatically affected benign upper GI surgery at our unit. Overall total operation numbers were down by 31% when compared to HD (440 vs 641). The largest deficit was in bariatrics where no bariatric surgery was performed during the first 12 months of the pandemic, which has restarted as of July 2021. There was also a 30% reduction in the number of cholecystectomies performed likely due to initial guidance recommending non-operative management at the start of the pandemic. Hiatal work numbers remained consistent. This quantitative study can direct future service delivery and help guide the post-pandemic recovery.
O-EGS07 Early initiation of biochemical venous thromboembolism prophylaxis following traumatic spleen injury is safe and effectively reduce VTE events
Authors: Rizkallah Georges , Lee Sheah Lin , Mahmoud Adel , Handa Ishada , Long Joe , Massella Virginia , Ngan Franko Shing Fun , Rahman Atiqur , Johns Jonathan , Modi Sachin , Elberm Hassan
Published: December 2021

Background

The standard of care for managing patients with traumatic splenic injuries (TSI) has become non operative management (NOM)1,3,4, but the safe window initiating chemical venous thromboembolism (VTE) prophylaxis, heparin or low molecular weight heparin (LMWH), is not well established 2. Within the first 48h from injury, hyper-coagulation state occurs which put trauma patients at risk of developing deep vein thrombosis(DVT), pulmonary embolism (PE) and lead to an increase rate in mortality 5,6. This study examines the safety and timing initiating VTE prophylaxis post splenic injury.

Methods

Patients with TSI were identified from prospectively maintained Trauma Audit and Research Network (TARN) database from 2015-2020 in a single tertiary trauma centre. Clinical and radio-logical information were collected retrospectively. TSI were graded using American Association for the Surgery of Trauma (AAST) splenic injury scale. Chemical venous thromboprophylaxis initiation were categorised as not given, <48h and >48h following the injury.

Results

In total 102 patient were included out of 136 patients identified with TSI. 34 patients were excluded for lack of electronic data, palliative decision or fatal condition on arrival. 12 patients out of 102 required operative management (OM) and 90 patients NOM. VTE prophylaxis was not given for 31 (30.4%). Medical reasons for this include severe brain injury and early discharge before 48 hours. VTE prophylaxis was initiated for 37 (36.3%) patients within 48 hours, and for 34 patients (33.3%) after 48 hours of admission. Seven patients developed thromboembolic events, majority of which (6/7) received VTE prophylaxis after 48 hours. Importantly, none of the patients who received VTE prophylaxis had rebleeding.

Conclusions

This study showed that early initiation of chemical VTE prophylaxis (<48h) is safe, resulted in lower incidence of DVTs/PEs without increasing the risk of bleeding. Results from this study supports recommendation from other studies 1 to initiate chemical VTE prophylaxis after TSI as early as 24h post injury with no other contra-indications