bluuebunny/crossref_metadata_2025 huggingface dataset:
Life cycle management of natural infrastructure: assessment of state of practice and current tools
Authors: Kurth Margaret H. , Piercy Candice D. , Jackson C. Rhett , Lemasson Bertrand H. , Harris Brian D.
Published: February 2024

Design alternatives for traditional infrastructure are often compared in terms of expected–and often narrowly defined–costs and benefits to justify the selected plan. Taking a broader life cycle perspective in the benefit-cost evaluation process helps account for potentially rare, indirect, or accruing project benefits. Natural infrastructure design alternatives are generally difficult to compare to conventional alternatives due to their distinctly different costs and benefits. Natural infrastructure differs from conventional infrastructure in terms of performance and benefit development over time, lifespan, materials, intensity of intervention needs, and social and environmental benefits. This paper presents a life cycle framework that expands conventional life cycle analysis to capture other important and relevant aspects of natural and conventional infrastructure, enabling a more complete and equitable comparison of project costs and benefits. The framework consists of four dimensions: risk mitigation performance (e.g., traditional benefit of flood risk management), co-benefits, financial costs (life cycle cost analysis), and environmental costs (life cycle assessment). The framework takes current benefit cost analysis practice for both infrastructure types into account, is informed by existing life cycle evaluation methods and tools and is responsive to the unique needs and characteristics of natural infrastructure. Components of this framework have been advanced elsewhere, including in business product management, asset management, building code development, environmental certifications, ecosystem goods and services accounting, and others, but are generally not developed for natural infrastructure. Our proposed framework provides a roadmap for development of supporting resources to conduct life cycle evaluation for natural infrastructure. Systematically grasping the temporal flow of costs and benefits of natural infrastructure, in comparison to conventional flood risk management projects, will be important as societies address vast infrastructure needs in the face of climate change.
Elevated remnant cholesterol and non-HDL cholesterol concentrations from real-world laboratory results: a cross-sectional study in Southeast Asians
Authors: Loh Wann Jia , Soh Heng Samuel , Tun Mon Hnin , Tan Pei Ting , Lau Chin Shern , Tavintharan Subramaniam , Watts Gerald F. , Aw Tar Choon
Published: February 2024

Introduction

Triglyceride-rich remnant lipoproteins (TRLs) are considered atherogenic due to the presence of remnant cholesterol, which is transported by apolipoprotein B. In clinical practice, the concentration of TRLs can be estimated by calculating remnant cholesterol or non-HDL cholesterol levels.

Aim

This study aims to investigate the proportion of patients who have low LDL cholesterol (LDL-C) concentration but elevated remnant cholesterol concentration, stratified by the presence of hypertriglyceridaemia and ethnicity, using real-world hospital data. Our secondary aim is to investigate the proportion of patients with elevated non-HDL cholesterol levels using guideline-recommended goals.

Methods

A 2-year retrospective study was conducted at a single centre, analyzing lipid blood tests of all patients, including directly measured LDL-C. Fasting for blood tests was not mandatory.

Results

The study included a total of 21,605 consecutive patients with plasma lipid profiles analyzed in our hospital laboratory. The median age was 61 years. In patients with ASCVD (n = 14,704), 23.7% had an LDL-C level of <1.8 mmol/L, 11.3% had elevated remnant cholesterol concentrations at ≥0.65 mmol/L, and 48.8% were at the non-high-density lipoprotein cholesterol (non-HDL-C) goal (<2.6 mmol/L). Among patients diagnosed with atherosclerotic cardiovascular disease (ASCVD) with LDL-C levels of <1.8 mmol/L (n = 3,484), only 11.9% had high levels of remnant cholesterol, but 96% of the ASCVD patients also achieved the recommended non-HDL-C target of <2.6 mmol/L. When the LDL-C level was <1.8 mmol/L, the mean concentration of remnant cholesterol was 0.214 mmol/L when the triglyceride level was <1.7 mmol/L (n = 3,380), vs. 0.70 mmol/L when the triglyceride level was elevated (n = 724), p < 0.001. Among patients with a triglyceride level of ≥1.7 mmol/L and an LDL-C level of <.8 mmol/L, there were 254 patients with elevated remnant cholesterol concentration and 71 patients with suboptimal non-HDL levels. Malays had a higher mean remnant cholesterol concentration compared with both Chinese and Indians across all LDL-C levels, particularly in the presence of hypertriglyceridaemia.

Conclusions

An elevated remnant cholesterol concentration of >0.65 mmol/L was present in 11% of all patients. The current guideline-recommended non-HDL-C goal, which uses a 0.8 mmol/L estimate of remnant cholesterol concentration, was achieved in >92% of patients, suggesting that it is unlikely to be clinically useful for the majority of our patient population except where there is concomitant hypertriglyceridaemia. Further studies are needed to establish the appropriate non-HDL-C goal or calculated remnant cholesterol concentration, paired with the LDL-C goal or otherwise, in a Southeast Asian population.
A customer satisfaction-based optimization model for the charging and discharging path and battery swapping stations’ site selection of electric vehicles
Authors: Zhang Yuhong , He Puyu , Ren Wenshi , Jiao Jie , Long Zhuhan , Jian Yaling
Published: February 2024

This paper studies the problem of considering customer satisfaction in the no-battery-swap mode and in the power-swap mode. First, with the goal of maximizing customer satisfaction, the total cost of charging and discharging and the minimum construction cost of swapping stations, the customer time window, and the load constraints of electric vehicles are considered. A model of electric vehicle charging and discharging route optimization and replacement station location without battery swapping behavior, considering customer satisfaction, is established, and then, a two-stage improved ant colony–genetic algorithm is designed to solve the model, and finally, the comparative analysis considers customer satisfaction. Based on the path optimization results and location decisions considering the cost of charging and discharging, the following conclusions are obtained: 1) electric vehicle route optimization and swap station location planning considering customer satisfaction can not only effectively reduce logistics distribution costs and replacement costs but also improve customer satisfaction levels. 2) Reducing the number of route crossings in the process of logistics distribution routes can save electricity costs for electric vehicles and logistics distribution costs, and help reduce the total cost of the entire logistics distribution network. 3) The gradient setting of the electricity price for electricity exchange will reduce the cost of electricity exchange, improve the utilization efficiency of the battery, reduce the cost of logistics and distribution, and improve the electricity exchange revenue of the electricity exchange station.
For environmental monitors, relationships matter in multiple ways: insights from a research collaboration in South Africa
Authors: Rosenberg Eureta , Mtati Nosiseko , Cockburn Jessica
Published: February 2024

The literature identifies several challenges facing natural resource management collaboration, from structural conditions like corruption to divergent interests, skewed decision-making powers and logistical, communications and information failures. The case study on which we base this paper examines a successful collaboration between university-based scientists and citizen environmental monitors in a rural region of South Africa. The Tsitsa project aimed to create benefits for people and environment, through collaborative research towards sustainable natural resource management. However, collaboration was not a given, and the lessons learnt in this regard form the gist of the paper. Using a relational realist lens, we conduct a secondary analysis of a case study undertaken in 2019–2020, into what the Tsitsa citizen monitors valued. It proved vital that researchers approached monitors in ways that communicated care, respect and trust, such as addressing them in their own language, being available for follow-ups, and paying for their work. When relational aspects were taken care of, collaboration flowed, and it was evident that citizen monitors share with scientists core human values: family, social standing through contribution, friendships and stimulating work, an interest in the environment and pride in a job well done. Our practice-based insights into the causal powers of relationships and the value of careful relationship-building for more sustainable and just natural resource management relationships adds to the emerging body of work on relationality in the sustainability sciences.
Similarity visualization of soundscapes in ecology and music
Authors: de Baudouin Adèle , Couprie Pierre , Michaud Félix , Haupert Sylvain , Sueur Jérôme
Published: February 2024

The concept of a soundscape is found in both ecology and music studies. Nature soundscapes and soundscape compositions are analyzed by both disciplines, respectively, to understand their biological diversity and ecosystem functioning and to interpret their compositional structure. A major challenge for both disciplines is visualizing the information embedded in a large variety of soundscapes and to share it with different audiences, from non-professionals to experts. To analyze soundscapes, both disciplines have independently developed similarity visualizations. However, no attempt has been made yet to combine these two fields of research to improve our ecological and musical perception of environmental sounds through shared similarity analysis methods. In this paper, we introduce a new visualization tool, the soundscape chord diagram (SCD), a circular similarity representation method that can be applied to any type of soundscape, either in ecoacoustics or electroacoustic studies. Our approach consists of visualizing spectral similarities between predefined sound segments based on the computation of a β-diversity acoustic index and on automatic clustering. SCDs were tested on two ecoacoustic forest databases and two electroacoustic soundscape compositions. SCDs were performant for the identification of specific acoustic events and highlighted known diel periods for nature soundscapes and written parts for soundscape compositions. This new visualization tool allows us to easily decipher the structure of musical and ecological acoustic data. SCDs could be applied to a large variety of soundscapes and promote their knowledge and preservation. This study opens a new way of investigating soundscapes at the interface between ecology and music, bringing together science and the arts.
Enhancement effects of mangrove restoration on blue carbon storage in Qinzhou Bay
Authors: Song Wentao , Hou Yukun , Zhu Wenjuan , Fan Yaocheng , Xu Haoyu , Cai Chengyu , Li Guanghao , Huang Lin
Published: February 2024

Introduction

Mangroves are the main carbon sinks in tropical regions and have high capabilities for carbon sequestration. Protection and restoration of mangroves are necessary to reduce carbon emissions and fight climate change. While the Qinzhou Bay as the main area of national mangrove restoration plan in the future, studies on its carbon pools, especially assessment of the carbon sink enhancement effect of restored mangroves along forest chronosequence, are lacking.

Methods

This study aimed to quantify the changes in restored mangrove soil carbon stock, vegetation and root carbon stocks along the forest age sequence in Qinzhou Bay through field survey.

Results

The results revealed that the carbon stocks of vegetation and roots significantly increased with the developing forest age. Only in the soil layer above 30 cm, the soil carbon storage apparently increased with the developing forest age in non-cofferdam area, and then decreased slowly after reaching the peak (at 6 ~ 8 years). Moreover, the soil carbon storage of mangroves was greater in the cofferdam area than in the non-cofferdam area.

Discussion

This implied that the cofferdam restoration efforts may be more effective in enhancing blue carbon storage, during the initial stages of the restoration process. The results of this study suggested that mangrove restoration has substantial potential capacity in carbon storage and nutrient cycling, providing a reference for the protection and restoration efforts concerning mangroves.
Influence of PCDH9 (rs9540720) and narcissistic personality traits on the incidence of major depressive disorder in Chinese first-year university students: findings from a 2-year cohort study
Authors: Xu Ruixue , Liu Zhaorui , Li Hanyun , Luo Linlin , Zheng Yi , Mu Fuqin , Liu Yujia , Zhang Weixin , Zhang Ying , Wang Jianli , Liu Yan
Published: February 2024

Objective: The objective of this study was to explore the influence of the polymorphism of the protocadherin 9 (PCDH9) gene and the narcissistic personality trait (NPT) on the risk of major depressive disorder (MDD) in Chinese first-year university students.

Methods: A 2-year cohort study was conducted among Chinese first-year university students who were enrolled in 2018 from two universities in Shandong Province, China. The snapshot technique was used to detect the genotypes of PCDH9 (rs9540720). The Chinese version of the Composite International Diagnostic Interview was used for the MDD assessment. The NPTs were measured by 11 items based on DSM-IV. Patient Health Questionnaire-9 and the Beck Anxiety Inventory were used to assess depressive and anxiety symptoms, respectively. Logistic regression modeling was carried out to examine the relationship between rs9540720, NPTs, and the incidence of MDD.

Results: A total of 5,327 students participated in the baseline and follow-up studies and provided their blood samples. PCDH9 (rs9540720) (ORGG+GA = 2.33, 95% CI: 1.35–4.02) and NPTs (OR5–9 = 2.26, 95% CI: 1.40–3.64) increased the risk of MDD onset. There was no multiplicative interaction between NPTs and Rs9540720 (OR = 1.51, 95% CI: 0.30–7.63). Furthermore, there was no additive interaction between them (RERI = 2.40, 95% CI: –0.82–5.62; AP = 0.47, 95% CI: –0.04–0.97; and S = 2.37, 95% CI: 0.54–10.33).

Conclusion:PCDH9 (rs9540720) and more NPTs are the risk factors for the incidence of MDD in Chinese first-year university students.
An approach to unified formulae for likelihood ratio calculation in pairwise kinship analysis
Authors: Ma Guanju , Wang Qian , Cong Bin , Li Shujin
Published: February 2024

Introduction: The likelihood ratio (LR) can be an efficient means of distinguishing various relationships in forensic fields. However, traditional list-based methods for derivation and presentation of LRs in distant or complex relationships hinder code editing and software programming. This paper proposes an approach for a unified formula for LRs, in which differences in participants’ genotype combinations can be ignored for specific identification. This formula could reduce the difficulty of by-hand coding, as well as running time of large-sample-size simulation.

Methods: The approach is first applied to a problem of kinship identification in which at least one of the participants is alleged to be inbred. This can be divided into two parts: i) the probability of different identical by descent (IBD) states according to the alleged kinship; and ii) the ratio of the probability that specific genotype combination can be detected assuming the alleged kinship exists between the two participants to the similar probability assuming that they are unrelated, for each state. For the probability, there are usually recognized results for common identification purposes. For the ratio, subscript letters representing IBD alleles of individual A’s alleles are used to eliminate differences in genotype combinations between the two individuals and to obtain a unified formula for the ratio in each state. The unification is further simplified for identification cases in which it is alleged that both of the participants are outbred. Verification is performed to show that the results obtained with the unified and list-form formulae are equivalent.

Results: A series of unified formulae are derived for different identification purposes, based on which an R package named KINSIMU has been developed and evaluated for use in large-size simulations for kinship analysis. Comparison between the package with two existing tools indicated that the unified approach presented here is more convenient and time-saving with respect to the coding process for computer applications compared with the list-based approach, despite appearing more complicated. Moreover, the method of derivation could be extended to other identification problems, such as those with different hypothesis sets or those involving multiple individuals.

Conclusion: The unified approach of LR calculation can be beneficial in kinship identification field.
Inflammatory cytokines and oral lichen planus: a Mendelian randomization study
Authors: Chen Xin , Zhang Simin , Wu Xiao , Lei Yuxi , Lei Bing , Zhao Zhibai
Published: February 2024

Background

Inflammatory cytokines have long been considered closely related to the development of oral lichen planus (OLP), and we further explored the causal relationship between the two by Mendelian randomization (MR) method.

Methods

We performed bidirectional MR analyses by large genome-wide association studies (GWAS). The data included a large-scale OLP dataset, as well as datasets of 41 inflammatory cytokines. All data were obtained from the University of Bristol database, which includes 41 inflammatory cytokines, and the GWAS Catalog database, which includes 91 inflammatory cytokines. OLP data were obtained from the Finngen database, which includes 6411 cases and 405770 healthy controls. We used the inverse variance weighted (IVW) method, MR-Egger method, weighted median method, simple mode method and weighted mode method to analyze the causal relationship between inflammatory cytokines and OLP, and we also combined with sensitivity analysis to further verify the robustness of the results. We performed a meta-analysis of positive or potentially positive results for the same genes to confirm the reliability of the final results.

Results

We primarily used the IVW analysis method, corrected using the Benjamin Hochberg (BH) method. When p<0.00038 (0.05/132), the results are significantly causal; when 0.00038<p<0.05, the results are potentially causal. We found a total of 7 inflammatory cytokines with significant or potential associations with OLP (University of Bristol database: 2, GWAS Catalog database: 5). In the reverse analysis, we found that a total of 30 inflammatory cytokines were significantly or potentially associated with OLP (University of Bristol database: 5, GWAS Catalog database: 25). After sensitivity analysis and meta-analysis, we finally determined that there was a causal relationship between a total of 3 inflammatory cytokines and OLP in the forward analysis, the most significant of which was FGF21 (p=0.02954, odds ratio (OR): 1.113, 95% confidence interval (95%CI): 1.011-1.226). In the reverse analysis, 14 inflammatory cytokines were causally associated with OLP, the most significant of which was PLAU (p=0.00002, OR: 0.951, 95%CI: 0.930-0.973).

Conclusion

There is a causal association between OLP and some inflammatory cytokines, which may play an important role in the pathogenesis of OLP and require further attention.
Ethical and legislative advances in xenotransplantation for clinical translation: focusing on cardiac, kidney and islet cell xenotransplantation
Authors: Hawthorne Wayne J.
Published: February 2024

In this state-of-the-art review we detail the journey of xenotransplantation from its infancy, detailing one of the first published cases and the subsequent journey the field took in its inception and development. With a focus on the science, technological advances, precautions required along with the potential limitations in application, the ethics, guidance’s, and legislative advances that are required to reach the safe and efficacious clinical application of xenotransplantation. Along with a view over the past several decades with the overall significant advancements in pre-clinical study outcomes particularly in islet, kidney, and heart xenotransplantation, to ultimately reach the pinnacle of successful clinical heart and kidney xenotransplants. It outlines the importance for the appropriate guidance’s required to have been developed by experts, scientists, clinicians, and other players who helped develop the field over the past decades. It also touches upon patient advocacy along with perspectives and expectations of patients, along with public opinion and media influence on the understanding and perception of xenotransplantation. It discusses the legislative environment in different jurisdictions which are reviewed in line with current clinical practices. All of which are ultimately based upon the guidance’s developed from a strong long-term collaboration between the International Xenotransplantation Association, the World Health Organisation and The Transplantation Society; each having constantly undertaken consultation and outreach to help develop best practice for clinical xenotransplantation application. These clearly helped forge the legislative frameworks required along with harmonization and standardization of regulations which are detailed here. Also, in relation to the significant advances in the context of initial xeno-kidney trials and the even greater potential for clinical xeno-islet trials to commence we discuss the significant advantages of xenotransplantation and the ultimate benefit to our patients.
Systematic analysis of MASP-1 serves as a novel immune-related biomarker in sepsis and trauma followed by preliminary experimental validation
Authors: Xian Lina , Cheng Shaowen , Chen Wei , Zhong Changhui , Hu Zhihua , Deng Xiaoyan
Published: February 2024

Background

Dysregulated immune response in trauma and sepsis leads to the abnormal activation of the complement and coagulation systems. Mannose-binding lectin (MBL)-associated serine protease-1 (MASP-1) activates the lectin pathway of the complement system and mediates proinflammatory and procoagulant reactions. However, the potential effects of MASP-1 in trauma and sepsis have not yet been explored.

Methods

We obtained five sepsis, two trauma, and one sepsis and trauma RNA-sequencing dataset from the Gene Expression Omnibus (GEO) database and conducted a comprehensive evaluation of the expression pattern, biological functions, and diagnostic value of MASP-1 in trauma and sepsis. Additionally, we investigated the association between MASP-1 expression and clinicopathological characteristics of trauma and sepsis. Furthermore, we collected clinical specimens to preliminarily validate the expression level and diagnostic efficacy of MASP-1 as well as the correlation of MASP-1 with clinical features of trauma and sepsis. Subsequently, we conducted a correlation analysis among MASP-1, immune cell infiltration, and immune and molecular pathways. Finally, we mechanistically analyzed the relationship among MASP-1, specific immune cells, and pivotal molecular pathways.

Results

MASP-1 expression was significantly upregulated in the trauma/sepsis samples compared to the control samples in the GEO datasets. MASP-1 exhibited excellent diagnostic values (AUC > 0.7) in multiple datasets and at multiple time points and could efficiently distinguish trauma/sepsis samples from the control samples. Moreover, MASP-1 expression was significantly positively correlated with the severity of the disease (APACHE-II, CRP, and neutrophil levels). These results were further validated by real-time quantitative polymerase chain reaction and enzyme-linked immunosorbent assay. Functional enrichment analysis revealed that MASP-1 primarily promotes trauma and sepsis via the immune-related signaling pathway. MASP-1 was significantly correlated with the infiltration of specific immune cells (such as B cells, CD8 T cells, neutrophils, macrophages, and infiltrating lymphocytes) and immune and molecular pathways (such as checkpoint, HLA, IL6/JAK/STAT3 signaling, necrosis, T-cell co-inhibition, and T-cell co-stimulation). Finally, analysis of the transcription and single-cell data revealed that MASP-1 was specifically expressed in T cells, and further correlation analysis revealed a close correlation between MASP-1 expression, proportion of CD8 T cells, and IL6/JAK/STAT3 signaling scores.

Conclusion

Our results suggest that MASP-1 can serve as an immune-related biomarker for the diagnosis and disease severity of trauma and sepsis. It may activate the IL6 JAK-STAT3 signaling pathway and promote CD8 T-cell depletion to trigger traumatic sepsis.
Oral_voting_transfer: classification of oral microorganisms’ function proteins with voting transfer model
Authors: Bao Wenzheng , Liu Yujun , Chen Baitong
Published: February 2024

Introduction

The oral microbial group typically represents the human body’s highly complex microbial group ecosystem. Oral microorganisms take part in human diseases, including Oral cavity inflammation, mucosal disease, periodontal disease, tooth decay, and oral cancer. On the other hand, oral microbes can also cause endocrine disorders, digestive function, and nerve function disorders, such as diabetes, digestive system diseases, and Alzheimer’s disease. It was noted that the proteins of oral microbes play significant roles in these serious diseases. Having a good knowledge of oral microbes can be helpful in analyzing the procession of related diseases. Moreover, the high-dimensional features and imbalanced data lead to the complexity of oral microbial issues, which can hardly be solved with traditional experimental methods.

Methods

To deal with these challenges, we proposed a novel method, which is oral_voting_transfer, to deal with such classification issues in the field of oral microorganisms. Such a method employed three features to classify the five oral microorganisms, including Streptococcus mutans, Staphylococcus aureus, abiotrophy adjacent, bifidobacterial, and Capnocytophaga. Firstly, we utilized the highly effective model, which successfully classifies the organelle’s proteins and transfers to deal with the oral microorganisms. And then, some classification methods can be treated as the local classifiers in this work. Finally, the results are voting from the transfer classifiers and the voting ones.

Results and discussion

The proposed method achieved the well performances in the five oral microorganisms. The oral_voting_transfer is a standalone tool, and all its source codes are publicly available at https://github.com/baowz12345/voting_transfer.
Benzalkonium chloride disinfectant residues stimulate biofilm formation and increase survival of Vibrio bacterial pathogens
Authors: Mougin Julia , Midelet Graziella , Leterme Sophie , Best Giles , Ells Timothy , Joyce Alyssa , Whiley Harriet , Brauge Thomas
Published: February 2024

Vibrio spp. are opportunistic human and animal pathogens found ubiquitously in marine environments. Globally, there is a predicted rise in the prevalence of Vibrio spp. due to increasing ocean temperatures, which carries significant implications for public health and the seafood industry. Consequently, there is an urgent need for enhanced strategies to control Vibrio spp. and prevent contamination, particularly in aquaculture and seafood processing facilities. Presently, these industries employ various disinfectants, including benzalkonium chloride (BAC), as part of their management strategies. While higher concentrations of BAC may be effective against these pathogens, inadequate rinsing post-disinfection could result in residual concentrations of BAC in the surrounding environment. This study aimed to investigate the adaptation and survival of Vibrio spp. exposed to varying concentrations of BAC residues. Results revealed that Vibrio bacteria, when exposed, exhibited a phenotypic adaptation characterized by an increase in biofilm biomass. Importantly, this effect was found to be strain-specific rather than species-specific. Exposure to BAC residues induced physiological changes in Vibrio biofilms, leading to an increase in the number of injured and alive cells within the biofilm. The exact nature of the “injured” bacteria remains unclear, but it is postulated that BAC might heighten the risk of viable but non-culturable (VBNC) bacteria development. These VBNC bacteria pose a significant threat, especially since they cannot be detected using the standard culture-based methods commonly employed for microbiological risk assessment in aquaculture and seafood industries. The undetected presence of VBNC bacteria could result in recurrent contamination events and subsequent disease outbreaks. This study provides evidence regarding the role of c-di-GMP signaling pathways in Vibrio adaptation mechanisms and suggests that c-di-GMP mediated repression is a potential avenue for further research. The findings underscore that the misuse and overuse of BAC may increase the risk of biofilm development and bacterial survival within the seafood processing chain.
Associations between plasma metal elements and risk of cognitive impairment among Chinese older adults
Authors: Wang Xin , Wang Binbin , Yang Fuwen , Shang Kaijian , Chen Shaowei , Zhang Yue
Published: February 2024

Background

The relationship between plasma metal elements and cognitive function is unclear, especially in extremely older individuals. This present study aimed to explore the association between plasma metal concentrations and the risk of cognitive impairment (CI) in Chinese extremely older adults.

Methods

Individuals aged ≥90 years with plasm metal concentration data from the fifth wave of the 2008 Chinese Longitudinal Healthy Longevity Survey were included. Plasma selenium (Se), manganese (Mn), magnesium (Mg), calcium (Ca), iron (Fe), copper (Cu), and zinc (Zn) concentrations were measured using inductively coupled plasma optical emission spectroscopy. Cognitive function was assessed by the Chinese version of the mini-mental state examination.

Results

The study enrolled 408 participants. Participants with CI had significantly lower plasma Se, Mn, and Fe levels and higher Ca levels than those with normal cognitive function (p < 0.05). Plasma Se, Mn, Ca, and Fe concentrations were significantly associated with CI risk in both single- and multiple-element logistic regression models. Additionally, the multiple-element model results showed that the adjusted odds ratios for CI were 0.042 (95% confidence interval 0.016–0.109), 0.106 (0.044–0.255), 7.629 (3.211–18.124) and 0.092 (0.036–0.233) for the highest quartiles compared to the lowest quartiles of Se, Mn, Ca, and Fe, respectively. Moreover, subgroup analyses by age, sex, and body mass index suggested a consistent significant correlation (p < 0.05).

Conclusion

Therefore, decreased plasma Se, Mn, and Fe and increased plasma Ca levels were associated with CI risk in Chinese older adults. These findings are of great significance for the development of programs to delay cognitive decline in the elderly.
Comprehensive analysis of genetic associations and single-cell expression profiles reveals potential links between migraine and multiple diseases: a phenome-wide association study
Authors: Ouyang Di , Huang Chunying , Liu Huihua , Xie Weiming , Chen Chengsheng , Su Ben , Guo Lizhong
Published: February 2024

Migraine is a common neurological disorder that affects more than one billion people worldwide. Recent genome-wide association studies have identified 123 genetic loci associated with migraine risk. However, the biological mechanisms underlying migraine and its relationships with other complex diseases remain unclear. We performed a phenome-wide association study (PheWAS) using UK Biobank data to investigate associations between migraine and 416 phenotypes. Mendelian randomization was employed using the IVW method. For loci associated with multiple diseases, pleiotropy was tested using MR-Egger. Single-cell RNA sequencing data was analyzed to profile the expression of 73 migraine susceptibility genes across brain cell types. qPCR was used to validate the expression of selected genes in microglia. PheWAS identified 15 disorders significantly associated with migraine, with one association detecting potential pleiotropy. Single-cell analysis revealed elevated expression of seven susceptibility genes (including ZEB2, RUNX1, SLC24A3, ANKDD1B, etc.) in brain glial cells. And qPCR confirmed the upregulation of these genes in LPS-treated microglia. This multimodal analysis provides novel insights into the link between migraine and other diseases. The single-cell profiling suggests the involvement of specific brain cells and molecular pathways. Validation of gene expression in microglia supports their potential role in migraine pathology. Overall, this study uncovers pleiotropic relationships and the biological underpinnings of migraine susceptibility.
Dominance hierarchy regulates social behavior during spatial movement
Authors: Lara-Vasquez Ariel , Espinosa Nelson , Morales Cristian , Moran Constanza , Billeke Pablo , Gallagher Joseph , Strohl Joshua J. , Huerta Patricio T. , Fuentealba Pablo
Published: February 2024

Rodents establish dominance hierarchy as a social ranking system in which one subject acts as dominant over all the other subordinate individuals. Dominance hierarchy regulates food access and mating opportunities, but little is known about its significance in other social behaviors, for instance during collective navigation for foraging or migration. Here, we implemented a simplified goal-directed spatial task in mice, in which animals navigated individually or collectively with their littermates foraging for food. We compared between conditions and found that the social condition exerts significant influence on individual displacement patterns, even when efficient navigation rules leading to reward had been previously learned. Thus, movement patterns and consequent task performance were strongly dependent on contingent social interactions arising during collective displacement, yet their influence on individual behavior was determined by dominance hierarchy. Dominant animals did not behave as leaders during collective displacement; conversely, they were most sensitive to the social environment adjusting their performance accordingly. Social ranking in turn was associated with specific spontaneous neural activity patterns in the prefrontal cortex and hippocampus, with dominant mice showing higher firing rates, larger ripple oscillations, and stronger neuronal entrainment by ripples than subordinate animals. Moreover, dominant animals selectively increased their cortical spiking activity during collective movement, while subordinate mice did not modify their firing rates, consistent with dominant animals being more sensitive to the social context. These results suggest that dominance hierarchy influences behavioral performance during contingent social interactions, likely supported by the coordinated activity in the hippocampal-prefrontal circuit.
Transcranial magneto-acoustic stimulation improves spatial memory and modulates hippocampal neural oscillations in a mouse model of Alzheimer's disease
Authors: Zhang Shuai , Guo Zhongsheng , Xu Yihao , Mi Jinrui , Liu Jun , Li Zichun , Xie Xiaofeng , Xu Guizhi
Published: February 2024

Introduction

In our study, we applied transcranial magneto-acoustic stimulation (TMAS), a technique based on focused ultrasound stimulation within a static magnetic field, in the APP/PS1 mouse model of Alzheimer's disease (AD) to explore the feasibility of TMAS on improving AD related spatial memory deficits and abnormal neural oscillations.

Methods

The mice treated with TMAS once daily for 21 days. We recorded local field potential signals in the hippocampal CA1 region of the mice after TMAS treatment with in-vivo electrophysiology and evaluated the neural rehabilitative effect of TMAS with sharp-wave ripple (SWR), gamma oscillations during SWRs, and phase-amplitude coupling (PAC). The spatial memory function of the mice was examined by the Morris water maze (MWM) task.

Results

We found that TMAS improved the performance of MWM related spatial cognitive functions compared with AD group. Furthermore, our results implied that TMAS alleviated abnormalities in hippocampal SWRs, increased slow gamma power during SWRs, and promoted theta-slow gamma phase-amplitude coupling. These findings suggest that TMAS could have a positive influence on spatial memory through the modulation of neural oscillations.

Discussion

This work emphasizes the potential of TMAS to serve as a non-invasive method for Alzheimer's disease rehabilitation and promote the application of TMAS for the treatment of more neurological and brain aging diseases in the future.
Urine Se concentration poorly predicts plasma Se concentration at sub-district scales in Zimbabwe, limiting its value as a biomarker of population Se status
Authors: Mutonhodza Beaula , Dembedza Mavis P. , Joy Edward J. M. , Manzeke-Kangara Muneta G. , Njovo Handrea , Nyadzayo Tasiana K. , Lark R. Murray , Kalimbira Alexander A. , Bailey Elizabeth H. , Broadley Martin R. , Matsungo Tonderayi M. , Chopera Prosper
Published: February 2024

Introduction

The current study investigated the value of urine selenium (Se) concentration as a biomarker of population Se status in rural sub-Saharan Africa.

Method

Urine and plasma Se concentrations were measured among children aged 6–59 months (n = 608) and women of reproductive age (WRA, n = 781) living in rural Zimbabwe (Murehwa, Shamva, and Mutasa districts) and participating in a pilot national micronutrient survey. Selenium concentrations were measured by inductively coupled plasma-mass spectrometry (ICP-MS), and urine concentrations were corrected for hydration status.

Results

The median (Q1, Q3) urine Se concentrations were 8.4 μg/L (5.3, 13.5) and 10.5 μg/L (6.5, 15.2) in children and WRA, respectively. There was moderate evidence for a relationship between urine Se concentration and plasma Se concentration in children (p = 0.0236) and WRA (p = < 0.0001), but the relationship had poor predictive value. Using previously defined thresholds for optimal activity of iodothyronine deiodinase (IDI), there was an association between deficiency when indicated by plasma Se concentrations and urine Se concentrations among WRA, but not among children.

Discussion

Urine Se concentration poorly predicted plasma Se concentration at sub-district scales in Zimbabwe, limiting its value as a biomarker of population Se status in this context. Further research is warranted at wider spatial scales to determine the value of urine Se as a biomarker when there is greater heterogeneity in Se exposure.
Case report: Safety and efficacy of synergistic treatment using selinexor and azacitidine in patients with atypical chronic myeloid leukemia with resistance to decitabine
Authors: Liu Lu , Song Xiaofeng , Dong Wenhao , Li Zhao , Guo Dongmei
Published: February 2024

Background

Atypical chronic myeloid leukemia (aCML) is a BCR::ABL1 negative myelodysplastic/myeloproliferative neoplasm with poor overall survival. Some patients can be treated by allogeneic hematopoietic stem cell transplantation (allo-HSCT) from suitable donors. The effectiveness of decitabine or azacitidine (AZA) has recently been reported; however, their combined efficacy with selinexor has not yet been reported.

Case description

In this study, we report the case of a patient with aCML who was successfully treated with selinexor combined with AZA. A 67-year-old man with a history of gastric mucosa-associated lymphoid tissue (MALT) lymphoma was admitted to the hospital with fatigue and emaciation. He was diagnosed with aCML and no longer responded to decitabine treatment after undergoing seven cycles. The patient was subsequently administered hydroxyurea (HU), selinexor, and AZA. After four courses of combination therapy, his blood cell counts improved; he no longer required transfusions and was able to discontinue HU. The patient continued receiving selinexor and AZA without severe complications. This case is the first to show that combinatorial selinexor and AZA therapy can effectively treat aCML.

Conclusion

Our case sheds light on the importance of selinexor and AZA combined therapy in the exploration of new treatment strategies for aCML. Moreover, this treatment approach offers the possibility of bridging with allo-HSCT.
Extracellular freezing induces a permeability transition in the inner membrane of muscle mitochondria of freeze-sensitive but not freeze-tolerant Chymomyza costata larvae
Authors: Štětina Tomáš , Koštál Vladimír
Published: February 2024

Background: Many insect species have evolved the ability to survive extracellular freezing. The search for the underlying principles of their natural freeze tolerance remains hampered by our poor understanding of the mechanistic nature of freezing damage itself.

Objectives: Here, in search of potential primary cellular targets of freezing damage, we compared mitochondrial responses (changes in morphology and physical integrity, respiratory chain protein functionality, and mitochondrial inner membrane (IMM) permeability) in freeze-sensitive vs. freeze-tolerant phenotypes of the larvae of the drosophilid fly, Chymomyza costata.

Methods: Larvae were exposed to freezing stress at −30°C for 1 h, which is invariably lethal for the freeze-sensitive phenotype but readily survived by the freeze-tolerant phenotype. Immediately after melting, the metabolic activity of muscle cells was assessed by the Alamar Blue assay, the morphology of muscle mitochondria was examined by transmission electron microscopy, and the functionality of the oxidative phosphorylation system was measured by Oxygraph-2K microrespirometry.

Results: The muscle mitochondria of freeze-tolerant phenotype larvae remained morphologically and functionally intact after freezing stress. In contrast, most mitochondria of the freeze-sensitive phenotype were swollen, their matrix was diluted and enlarged in volume, and the structure of the IMM cristae was lost. Despite this morphological damage, the electron transfer chain proteins remained partially functional in lethally frozen larvae, still exhibiting strong responses to specific respiratory substrates and transferring electrons to oxygen. However, the coupling of electron transfer to ATP synthesis was severely impaired. Based on these results, we formulated a hypothesis linking the observed mitochondrial swelling to a sudden loss of barrier function of the IMM.
Callose in leptoid cell walls of the moss Polytrichum and the evolution of callose synthase across bryophytes
Authors: Renzaglia Karen , Duran Emily , Sagwan-Barkdoll Laxmi , Henry Jason
Published: February 2024

Introduction

Leptoids, the food-conducting cells of polytrichaceous mosses, share key structural features with sieve elements in tracheophytes, including an elongated shape with oblique end walls containing modified plasmodesmata or pores. In tracheophytes, callose is instrumental in developing the pores in sieve elements that enable efficient photoassimilate transport. Aside from a few studies using aniline blue fluorescence that yielded confusing results, little is known about callose in moss leptoids.

Methods

Callose location and abundance during the development of leptoid cell walls was investigated in the moss Polytrichum commune using aniline blue fluorescence and quantitative immunogold labeling (label density) in the transmission electron microscope. To evaluate changes during abiotic stress, callose abundance in leptoids of hydrated plants was compared to plants dried for 14 days under field conditions. A bioinformatic study to assess the evolution of callose within and across bryophytes was conducted using callose synthase (CalS) genes from 46 bryophytes (24 mosses, 15 liverworts, and 7 hornworts) and one representative each of five tracheophyte groups.

Results

Callose abundance increases around plasmodesmata from meristematic cells to end walls in mature leptoids. Controlled drying resulted in a significant increase in label density around plasmodesmata and pores over counts in hydrated plants. Phylogenetic analysis of the CalS protein family recovered main clades (A, B, and C). Different from tracheophytes, where the greatest diversity of homologs is found in clade A, the majority of gene duplication in bryophytes is in clade B.

Discussion

This work identifies callose as a crucial cell wall polymer around plasmodesmata from their inception to functioning in leptoids, and during water stress similar to sieve elements of tracheophytes. Among bryophytes, mosses exhibit the greatest number of multiple duplication events, while only two duplications are revealed in hornwort and none in liverworts. The absence in bryophytes of the CalS 7 gene that is essential for sieve pore development in angiosperms, reveals that a different gene is responsible for synthesizing the callose associated with leptoids in mosses.
Corporal punishment and violent behavior spectrum: a meta-analytic review
Authors: Pan Qingna , Chen Siru , Qu Ying
Published: February 2024

Corporal punishment is believed to precede various forms of violent behavior, yet prior research has yielded inconsistent findings, partly due to variations in violent types and other factors. This meta-analysis systematically reviewed 35 studies including 144 effect sizes (comprising a total sample size of 159,213) investigating the association between corporal punishment and a spectrum of violent behaviors called Violent Behavior Spectrum (VBS). Additionally, meta-regressions were conducted to explore the moderating impact of punishment severity, violence type and cultural context. Our findings indicated a significant positive relationship between corporal punishment and VBS (r = 0.238, 95%, CI [0.176, 0.300]). Notably, punishment severity was found to influence the strength of this association. Namely, The more severe the corporal punishment, the more likely it is to lead to VBS. These results enhance our understanding of the intricate connection between corporal punishment and various forms of violence, providing valuable insights for both parenting practices and policy development.
Impact of the COVID-19 pandemic on the prevalence of respiratory viral pathogens in patients with acute respiratory infection in Shanghai, China
Authors: Pan Lifeng , Yuan Yang , Cui Qiqi , Zhang Xuechun , Huo Yujia , Liu Qing , Zou Wenwei , Zhao Bing , Hao Lipeng
Published: February 2024

Objective

This study aimed to evaluate the impact of nonpharmaceutical interventions (NPIs) taken to combat COVID-19 on the prevalence of respiratory viruses (RVs) of acute respiratory infections (ARIs) in Shanghai.

Methods

Samples from ARI patients were collected and screened for 17 respiratory viral pathogens using TagMan low density microfluidic chip technology in Shanghai from January 2019 to December 2020. Pathogen data were analyzed to assess changes in acute respiratory infections between 2019 and 2020.

Results

A total of 2,744 patients were enrolled, including 1,710 and 1,034 in 2019 and 2020, respectively. The total detection rate of RVs decreased by 149.74% in 2020. However, detection rates for human respiratory syncytial virus B (RSVB), human coronavirus 229E (HCoV229E), human coronavirus NL63 (HCoVNL63), and human parainfluenza virus 3 (HPIV3) increased by 91.89, 58.33, 44.68 and 24.29%, in 2020. The increased positive rates of RSVB, HPIV3, resulted in more outpatients in 2020 than in 2019. IFV detection rates declined dramatically across gender, age groups, and seasons in 2020.

Conclusion

NPIs taken to eliminate COVID-19 had an impact on the prevalence of respiratory viral pathogens, especially the IFVs in the early phases of the pandemic. Partial respiratory viruses resurged with the lifting of NPIs, leading to an increase in ARIs infection.