bluuebunny/crossref_metadata_2025 huggingface dataset:
Lankesterella (Apicomplexa, Lankesterellidae) Blood Parasites of Passeriform Birds: Prevalence, Molecular and Morphological Characterization, with Notes on Sporozoite Persistence In Vivo and Development In Vitro
Authors: Chagas Carolina Romeiro Fernandes , Harl Josef , Preikša Vytautas , Bukauskaitė Dovilė , Ilgūnas Mikas , Weissenböck Herbert , Valkiūnas Gediminas
Published: May 2021

Recent studies confirmed that some Hepatozoon-like blood parasites (Apicomplexa) of birds are closely related to the amphibian parasite Lankesterella minima. Little is known about the biology of these pathogens in birds, including their distribution, life cycles, specificity, vectors, and molecular characterization. Using blood samples of 641 birds from 16 species, we (i) determined the prevalence and molecular diversity of Lankesterella parasites in naturally infected birds; (ii) investigated the development of Lankesterella kabeeni in laboratory-reared mosquitoes, Culex pipiens forma molestus and Aedes aegypti; and (iii) tested experimentally the susceptibility of domestic canaries, Serinus canaria, to this parasite. This study combined molecular and morphological diagnostic methods and determined 11% prevalence of Lankesterella parasites in Acrocephalidae birds; 16 Lankesterella lineages with a certain degree of host specificity and two new species (Lankesterella vacuolata n. sp. and Lankesterella macrovacuolata n. sp.) were found and characterized. Lankesterella kabeeni (formerly Hepatozoon kabeeni) was re-described. Serinus canaria were resistant after various experimental exposures. Lankesterella sporozoites rapidly escaped from host cells in vitro. Sporozoites persisted for a long time in infected mosquitoes (up to 42 days post exposure). Our study demonstrated a high diversity of Lankesterella parasites in birds, and showed that several avian Hepatozoon-like parasites, in fact, belong to Lankesterella genus.
Caffeic Acid Phenyl Ester (CAPE) Protects against Iron-Mediated Cellular DNA Damage through Its Strong Iron-Binding Ability and High Lipophilicity
Authors: Shao Bo , Mao Li , Tang Miao , Yan Zhu-Ying , Shao Jie , Huang Chun-Hua , Sheng Zhi-Guo , Zhu Ben-Zhan
Published: May 2021

Caffeic acid phenethyl ester (CAPE) and its structurally-related caffeic acid (CA), ferulic acid (FA) and ethyl ferulate (EF) are constituents of honeybee propolis that have important pharmacological activities. This study found that CAPE—but not CA, FA, and EF—could effectively prevent cellular DNA damage induced by overloaded iron through decreasing the labile iron pool (LIP) levels in HeLa cells. Interestingly, CAPE was found to be more effective than CA in protecting against plasmid DNA damage induced by Fe(II)–H2O2 or Fe(III)–citrate–ascorbate-H2O2 via the inhibition of hydroxyl radical (•OH) production. We further provided more direct and unequivocal experimental evidences for the formation of inactive CAPE/CA–iron complexes. CAPE was found to have a stronger iron-binding ability and a much higher lipophilicity than CA. Taken together, we propose that the esterification of the carboxylic moiety with phenethyl significantly enhanced the iron-binding ability and lipophilicity of CAPE, which is also responsible for its potent protection against iron-mediated cellular DNA damage. A study on the iron coordination mechanism of such natural polyphenol antioxidants will help to design more effective antioxidants for the treatment and prevention of diseases caused by metal-induced oxidative stress, as well as help to understand the structure–activity relationships of these compounds.
The Pro-Oxidant Activity of Red Wine Polyphenols Induces an Adaptive Antioxidant Response in Human Erythrocytes
Authors: Tedesco Idolo , Spagnuolo Carmela , Russo Gian Luigi , Russo Maria , Cervellera Carmen , Moccia Stefania
Published: May 2021

The protective effect of dealcoholized red wine on human health has been partially associated with its polyphenolic components, suggesting that the pool of polyphenols, including flavonoids and anthocyanins, can be responsible for the functional effects of this beverage. We hypothesize a new role of red wine polyphenols (RWp) in modulating the antioxidant potential of erythrocytes, protecting them against oxidative stress. We previously demonstrated that RWp activated the Plasma Membrane Redox System (PMRS), which is involved in neutralizing plasma free radicals. Here, we investigated the underlying mechanism triggered by RWp in the activation of PMRS via the involvement of GSH. Hence, treatment of human erythrocytes with RWp (73 μg/mL Gallic Acid Equivalents) increased GSH intracellular concentration, which depends upon the activation of glutathione reductase (GR) and glucose-6-phosphate dehydrogenase (G6PD), whose enzymatic activities increase of about 30% and 47%, respectively. Changes in the GSH pathway induced by RWp were associated with a slight but significant increase in reactive oxygen species (ROS). We conclude that the pro-oxidant effect of RWp promoted an adaptive stress response in human erythrocytes, which enhances their antioxidant defense.
Traffic Monitoring System Based on Deep Learning and Seismometer Data
Authors: Ahmad Ahmad Bahaa , Tsuji Takeshi
Published: May 2021

Currently, vehicle classification in roadway-based techniques depends mainly on photos/videos collected by an over-roadway camera or on the magnetic characteristics of vehicles. However, camera-based techniques are criticized for potentially violating the privacy of vehicle occupants and exposing their identity, and vehicles can evade detection when they are obscured by larger vehicles. Here, we evaluate methods of identifying and classifying vehicles on the basis of seismic data. Vehicle identification from seismic signals is considered a difficult task because of interference by various noise. By analogy with techniques used in speech recognition, we used different artificial intelligence techniques to extract features of three, different-sized vehicles (buses, cars, motorcycles) and seismic noise. We investigated the application of a deep neural network (DNN), a convolutional neural network (CNN), and a recurrent neural network (RNN) to classify vehicles on the basis of vertical-component seismic data recorded by geophones. The neural networks were trained on 5580 unprocessed seismic records and achieved excellent training accuracy (99%). They were also tested on large datasets representing periods as long as 1 month to check their stability. We found that CNN was the most satisfactory approach, reaching 96% accuracy and detecting multiple vehicle classes at the same time at a low computational cost. Our findings show that seismic methods can be used for traffic monitoring and security purposes without violating the privacy of vehicle occupants, offering greater efficiency and lower costs than current methods. A similar approach may be useful for other types of transportation, such as vessels and airplanes.
A Location Privacy Preservation Method Based on Dummy Locations in Internet of Vehicles
Authors: Xu Xianyun , Chen Huifang , Xie Lei
Published: May 2021

During the procedure, a location-based service (LBS) query, the real location provided by the vehicle user may results in the disclosure of vehicle location privacy. Moreover, the point of interest retrieval service requires high accuracy of location information. However, some privacy preservation methods based on anonymity or obfuscation will affect the service quality. Hence, we study the location privacy-preserving method based on dummy locations in this paper. We propose a vehicle location privacy-preservation method based on dummy locations under road restriction in Internet of vehicles (IoV). In order to improve the validity of selected dummy locations under road restriction, entropy is used to represent the degree of anonymity, and the effective distance is introduced to represent the characteristics of location distribution. We present a dummy location selection algorithm to maximize the anonymous entropy and the effective distance of candidate location set consisting of vehicle user’s location and dummy locations, which ensures the uncertainty and dispersion of selected dummy locations. The proposed location privacy-preservation method does not need a trustable third-party server, and it protects the location privacy of vehicles as well as guaranteeing the LBS quality. The performance analysis and simulation results show that the proposed location privacy-preservation method can improve the validity of dummy locations and enhance the preservation of location privacy compared with other methods based on dummy locations.
Strength and Durability of Hybrid Fiber-Reinforced Latex-Modified Rapid-Set Cement Preplaced Concrete for Emergency Concrete Pavement Repair
Authors: Lee Su-Jin , Shin Hyung-Jin , Park Chan-Gi
Published: May 2021

The benefits of using reinforcing fibers in latex-modified rapid-set cement preplaced concrete for emergency pavement repairs were examined in terms of strength, permeability, and durability as functions of the type of fiber. Single-type fibers, including jute, poly (vinyl alcohol) (PVA), and nylon fibers, as well as hybrid fiber mixtures prepared with two of the aforementioned fibers at a 1:1 weight ratio, were evaluated. Fibers were incorporated into the concrete mixture at 1.2 kg/m3. A vibratory press compactor that simulates roller compaction was used to increase compaction and densification of the resulting pavement repair material. The hybrid fiber-reinforced latex-modified rapid-set cement preplaced concrete (HFLMC) was manufactured to satisfy the criteria for opening traffic, i.e., compressive strength of 21 MPa or higher, and flexural strength of 3.5 MPa or higher after 4 h. Pavement requiring repair was removed and replaced with coarse aggregate. The rapid-set binder, fibers, and latex were then mixed and placed onto the coarse aggregate layer. The repair was considered complete after compaction. The resulting HFLMC satisfied all of the test criteria. Furthermore, concretes made with hybrid fibers were more mechanically sound than those made with a single fiber variety. Hybrid fiber concretes made with PVA and nylon fibers exhibited the best properties for emergency pavement repair. These results indicate that HFLMC is suitable for emergency pavement repair.
Roto-Translational Control of Spacecraft in Low Earth Orbit Using Environmental Forces and Torques
Authors: Riano-Rios Camilo , Fedele Alberto , Bevilacqua Riccardo
Published: May 2021

In this paper, relative orbit and attitude adaptive controllers are integrated to perform roto-translational maneuvers for CubeSats equipped with a Drag Maneuvering Device (DMD). The DMD enables the host CubeSat with modulation of aerodynamic forces/torques and gravity gradient torque. Adaptive controllers for independent orbital and attitude maneuvers are revisited to account for traslational-attitude coupling while compensating for uncertainty in parameters such as atmospheric density, drag/lift coefficients, location of the Center of Mass (CoM) and inertia matrix. Uniformly ultimately bounded convergence of the attitude error and relative orbit states is guaranteed by Lyapunov-based stability analysis for the integrated roto-translational maneuver. A simulation example of an along-track formation maneuver between two CubeSats with simultaneous attitude control using only environmental forces and torques is presented to validate the controller.
Method for Viewing Real-World Scenes while Recording Video
Authors: Park Daehee , Lee Cheoljun
Published: May 2021

Because smartphones support various functions, they are carried by users everywhere. Whenever a user believes that a moment is interesting, important, or meaningful to them, they can record a video to preserve such memories. The main problem with video recording an important moment is the fact that the user needs to look at the scene through the mobile phone screen rather than seeing the actual real-world event. This occurs owing to uncertainty the user might feel when recording the video. For example, the user might not be sure if the recording is of high-quality and might worry about missing the target object. To overcome this, we developed a new camera application that utilizes two main algorithms, the minimum output sum of squared error and the histograms of oriented gradient algorithms, to track the target object and recognize the direction of the user’s head. We assumed that the functions of the new camera application can solve the user’s anxiety while recording a video. To test the effectiveness of the proposed application, we conducted a case study and measured the emotional responses of users and the error rates based on a comparison with the use of a regular camera application. The results indicate that the new camera application induces greater feelings of pleasure, excitement, and independence than a regular camera application. Furthermore, it effectively reduces the error rates during video recording.
Geochemical Characterization and Heavy Metal Sources in PM10 in Arequipa, Peru
Authors: Li Jianghanyang , Michalski Greg , Olson Elizabeth Joy , Welp Lisa R. , Larrea Valdivia Adriana E. , Larico Juan Reyes , Zapata Francisco Alejo , Paredes Lino Morales
Published: May 2021

Particulate matter smaller than 10 μm (PM10) is an important air pollutant that adversely affects human health by increasing the risk of respiratory and cardiovascular diseases. Recent studies reported multiple extreme PM10 levels at high altitude Peruvian cities, which resulted from a combination of high emissions and limited atmospheric circulation at high altitude. However, the emission sources of the PM10 still remain unclear. In this study, we collected PM10 samples from four sites (one industrial site, one urban site, and two rural sites) at the city of Arequipa, Peru, during the period of February 2018 to December 2018. To identify the origins of PM10 at each site and the spatial distribution of PM10 emission sources, we analyzed major and trace element concentrations of the PM10. Of the observed daily PM10 concentrations at Arequipa during our sampling period, 91% exceeded the World Health Organization (WHO) 24-h mean PM10 guideline value, suggesting the elevated PM10 strongly affected the air quality at Arequipa. The concentrations of major elements, Na, K, Mg, Ca, Fe, and Al, were high and showed little variation, suggesting that mineral dust was a major component of the PM10 at all the sites. Some trace elements, such as Mn and Mo, originated from the mineral dust, while other trace elements, including Pb, Sr, Cu, Ba, Ni, As and V, were from additional anthropogenic sources. The industrial activities at Rio Seco, the industrial site, contributed to significant Pb, Cu, and possibly Sr emissions. At two rural sites, Tingo Grande and Yarabamba, strong Cu emissions were observed, which were likely associated with mining activities. Ni, V, and As were attributed to fossil fuel combustion emissions, which were strongest at the Avenida Independencia urban site. Elevated Ba and Cu concentrations were also observed at the urban site, which were likely caused by heavy traffic in the city and vehicle brake wear emissions.
Review of Bone Conduction Hearing Devices
Authors: Ellsperman Susan E. , Nairn Emily M. , Stucken Emily Z.
Published: May 2021

Bone conduction is an efficient pathway of sound transmission which can be harnessed to provide hearing amplification. Bone conduction hearing devices may be indicated when ear canal pathology precludes the use of a conventional hearing aid, as well as in cases of single-sided deafness. Several different technologies exist which transmit sound via bone conduction. Here, we will review the physiology of bone conduction, the indications for bone conduction amplification, and the specifics of currently available devices.
Iodine Map Radiomics in Breast Cancer: Prediction of Metastatic Status
Authors: Lenga Lukas , Bernatz Simon , Martin Simon S. , Booz Christian , Solbach Christine , Mulert-Ernst Rotraud , Vogl Thomas J. , Leithner Doris
Published: May 2021

Dual-energy CT (DECT) iodine maps enable quantification of iodine concentrations as a marker for tissue vascularization. We investigated whether iodine map radiomic features derived from staging DECT enable prediction of breast cancer metastatic status, and whether textural differences exist between primary breast cancers and metastases. Seventy-seven treatment-naïve patients with biopsy-proven breast cancers were included retrospectively (41 non-metastatic, 36 metastatic). Radiomic features including first-, second-, and higher-order metrics as well as shape descriptors were extracted from volumes of interest on iodine maps. Following principal component analysis, a multilayer perceptron artificial neural network (MLP-NN) was used for classification (70% of cases for training, 30% validation). Histopathology served as reference standard. MLP-NN predicted metastatic status with AUCs of up to 0.94, and accuracies of up to 92.6 in the training and 82.6 in the validation datasets. The separation of primary tumor and metastatic tissue yielded AUCs of up to 0.87, with accuracies of up to 82.8 in the training, and 85.7 in the validation dataset. DECT iodine map-based radiomic signatures may therefore predict metastatic status in breast cancer patients. In addition, microstructural differences between primary and metastatic breast cancer tissue may be reflected by differences in DECT radiomic features.
Loss of PGRMC1 Delays the Progression of Hepatocellular Carcinoma via Suppression of Pro-Inflammatory Immune Responses
Authors: Lee Sang R. , Lee Jong Geol , Heo Jun H. , Jo Seong Lae , Ryu Jihoon , Kim Globinna , Yon Jung-Min , Lee Myeong Sup , Lee Geun-Shik , An Beum-Soo , Shin Hyun-Jin , Woo Dong-Cheol , Baek In-Jeoung , Hong Eui-Ju
Published: May 2021

Pgrmc1 is a non-canonical progesterone receptor related to the lethality of various types of cancer. PGRMC1 has been reported to exist in co-precipitated protein complexes with epidermal growth factor receptor (EGFR), which is considered a useful therapeutic target in hepatocellular carcinoma (HCC). Here, we investigated whether Pgrmc1 is involved in HCC progression. In clinical datasets, PGRMC1 transcription level was positively correlated with EGFR levels; importantly, PGRMC1 level was inversely correlated with the survival duration of HCC patients. In a diethylnitrosamine (DEN)-induced murine model of HCC, the global ablation of Pgrmc1 suppressed the development of HCC and prolonged the survival of HCC-bearing mice. We further found that increases in hepatocyte death and suppression of compensatory proliferation in the livers of DEN-injured Pgrmc1-null mice were concomitant with decreases in nuclear factor κB (NF-κB)-dependent production of interleukin-6 (IL-6). Indeed, silencing of Pgrmc1 in murine macrophages led to reductions in NF-κB activity and IL-6 production. We found that the anti-proinflammatory effect of Pgrmc1 loss was mediated by reductions in EGFR level and its effect was not observed after exposure of the EGFR inhibitor erlotinib. This study reveals a novel cooperative role of Pgrmc1 in supporting the EGFR-mediated development of hepatocellular carcinoma, implying that pharmacological suppression of Pgrmc1 may be a useful strategy in HCC treatment.
Discovery and Evaluation of Protein Biomarkers as a Signature of Wellness in Late-Stage Cancer Patients in Early Phase Clinical Trials
Authors: Geary Bethany , Peat Erin , Dransfield Sarah , Cook Natalie , Thistlethwaite Fiona , Graham Donna , Carter Louise , Hughes Andrew , Krebs Matthew G. , Whetton Anthony D.
Published: May 2021

TARGET (tumour characterisation to guide experimental targeted therapy) is a cancer precision medicine programme focused on molecular characterisation of patients entering early phase clinical trials. Performance status (PS) measures a patient’s ability to perform a variety of activities. However, the quality of present algorithms to assess PS is limited and based on qualitative clinician assessment. Plasma samples from patients enrolled into TARGET were analysed using the mass spectrometry (MS) technique: sequential window acquisition of all theoretical fragment ion spectra (SWATH)-MS. SWATH-MS was used on a discovery cohort of 55 patients to differentiate patients into either a good or poor prognosis by creation of a Wellness Score (WS) that showed stronger prediction of overall survival (p = 0.000551) compared to PS (p = 0.001). WS was then tested against a validation cohort of 77 patients showing significant (p = 0.000451) prediction of overall survival. WS in both sets had receiver operating characteristic curve area under the curve (AUC) values of 0.76 (p = 0.002) and 0.67 (p = 0.011): AUC of PS was 0.70 (p = 0.117) and 0.55 (p = 0.548). These signatures can now be evaluated further in larger patient populations to assess their utility in a clinical setting.
Online, Group-Based Psychological Support for Adolescent and Young Adult Cancer Survivors: Results from the Recapture Life Randomized Trial
Authors: Sansom-Daly Ursula , Wakefield Claire , Ellis Sarah , McGill Brittany , Donoghoe Mark , Butow Phyllis , Bryant Richard , Sawyer Susan , Patterson Pandora , Anazodo Antoinette , Plaster Megan , Thompson Kate , Holland Lucy , Osborn Michael , Maguire Fiona , O’Dwyer Catherine , De Abreu Lourenco Richard , Cohn Richard ,   The Recapture Life Working Party
Published: May 2021

Telehealth interventions offer a practical platform to support adolescent and young adult (AYA) cancer survivors’ mental health needs after treatment, yet efficacy data are lacking. We evaluated an online, group-based, videoconferencing-delivered cognitive-behavioral therapy (CBT) intervention (‘Recapture Life’) in a 3-arm randomized-controlled trial comparing Recapture Life with an online peer-support group, and a waitlist control, with the aim of testing its impact on quality of life, emotional distress and healthcare service use. Forty AYAs (Mage = 20.6 years) within 24-months of completing treatment participated, together with 18 support persons. No groupwise impacts were measured immediately after the six-week intervention. However, Recapture Life participants reported using more CBT skills at the six-week follow-up (OR = 5.58, 95% CI = 2.00–15.56, p = 0.001) than peer-support controls. Recapture Life participants reported higher perceived negative impact of cancer, anxiety and depression at 12-month follow-up, compared to peer-support controls. Post-hoc analyses suggested that AYAs who were further from completing cancer treatment responded better to Recapture Life than those who had completed treatment more recently. While online telehealth interventions hold promise, recruitment to this trial was challenging. As the psychological challenges of cancer survivorship are likely to evolve with time, different support models may prove more or less helpful for different sub-groups of AYA survivors at different times.
A Hybrid In Silico and Tumor-on-a-Chip Approach to Model Targeted Protein Behavior in 3D Microenvironments
Authors: Palacio-Castañeda Valentina , Dumas Simon , Albrecht Philipp , Wijgers Thijmen J. , Descroix Stéphanie , Verdurmen Wouter P. R.
Published: May 2021

To rationally improve targeted drug delivery to tumor cells, new methods combining in silico and physiologically relevant in vitro models are needed. This study combines mathematical modeling with 3D in vitro co-culture models to study the delivery of engineered proteins, called designed ankyrin repeat proteins (DARPins), in biomimetic tumor microenvironments containing fibroblasts and tumor cells overexpressing epithelial cell adhesion molecule (EpCAM) or human epithelial growth factor receptor (HER2). In multicellular tumor spheroids, we observed strong binding-site barriers in combination with low apparent diffusion coefficients of 1 µm2·s−1 and 2 µm2 ·s−1 for EpCAM- and HER2-binding DARPin, respectively. Contrasting this, in a tumor-on-a-chip model for investigating delivery in real-time, transport was characterized by hindered diffusion as a consequence of the lower local tumor cell density. Finally, simulations of the diffusion of an EpCAM-targeting DARPin fused to a fragment of Pseudomonas aeruginosa exotoxin A, which specifically kills tumor cells while leaving fibroblasts untouched, correctly predicted the need for concentrations of 10 nM or higher for extensive tumor cell killing on-chip, whereas in 2D models picomolar concentrations were sufficient. These results illustrate the power of combining in vitro models with mathematical modeling to study and predict the protein activity in complex 3D models.
A Three-Component Hybrid Templated by Asymmetric Viologen Exhibiting Visible-Light-Driven Photocatalytic Degradation on Dye Pollutant in Maritime Accident Seawater
Authors: Zhuang Xueqiang , Wu Qiqi , Huang Xihe , Li Haohong , Lin Tianjin , Gao Yali
Published: May 2021

The increasing dangerous chemical pollutants led by shipping accidents call for the new pollutant treatment strategy. In this work, a new three-component hybrid {[(BiI6)I13]·2I3·(H-BPA)4}n (1) can be used in dye degradation in seawater. The highly interesting feature of 1 lies in its unique 1-D Z-shape [(BiI6)I13]n6− infinite chain constructed from the I···I contacts between mono-nuclear (BiI6)3− anions and I133− polyiodide anions. Finally, the hydrogen bonds between [(BiI6)I13]n6− polyanions and H-BPA2+ cations contribute to the formation a quasi-3-D network. Specifically, 1 exhibits the wide absorption zone from ultraviolet to visible regions and high charge-separation efficiency, hinting its application in visible-light catalysis. As expected, 1 represents photocatalytic activity for the degradation of rhodamine B in seawater with degradation ratio of 90%, and the photocatalytic performance is stable. This work might provide new photocatalytic material for pollutant treatment in shipping accidents.
Influence of Co-Precipitation Agent on the Structure, Texture and Catalytic Activity of Au-CeO2 Catalysts in Low-Temperature Oxidation of Benzyl Alcohol
Authors: Wolski Lukasz , Nowaczyk Grzegorz , Jurga Stefan , Ziolek Maria
Published: May 2021

The aim of the study was to establish the influence of a co-precipitation agent (i.e., NaOH–immediate precipitation; hexamethylenetetramine/urea–gradual precipitation and growth of nanostructures) on the properties and catalytic activity of as-synthesized Au-CeO2 nanocomposites. All catalysts were fully characterized with the use of XRD, nitrogen physisorption, ICP-OES, SEM, HR-TEM, UV-vis, XPS, and tested in low-temperature oxidation of benzyl alcohol as a model oxidation reaction. The results obtained in this study indicated that the type of co-precipitation agent has a significant impact on the growth of gold species. Immediate co-precipitation of Au-CeO2 nanostructures with the use of NaOH allowed obtainment of considerably smaller and more homogeneous in size gold nanoparticles than those formed by gradual co-precipitation and growth of Au-CeO2 nanostructures in the presence of hexamethylenetetramine or urea. In the catalytic tests, it was established that the key factor promoting high activity in low-temperature oxidation of benzyl alcohol was size of gold nanoparticles. The highest conversion of the alcohol was observed for the catalyst containing the smallest Au particle size (i.e., Au-CeO2 nanocomposite prepared with the use of NaOH as a co-precipitation agent).
Ablation of TRPV1 Abolishes Salicylate-Induced Sympathetic Activity Suppression and Exacerbates Salicylate-Induced Renal Dysfunction in Diet-Induced Obesity
Authors: Zhong Beihua , Ma Shuangtao , Wang Donna H.
Published: May 2021

Sodium salicylate (SA), a cyclooxygenase inhibitor, has been shown to increase insulin sensitivity and to suppress inflammation in obese patients and animal models. Transient receptor potential vanilloid 1 (TRPV1) is a nonselective cation channel expressed in afferent nerve fibers. Cyclooxygenase-derived prostaglandins are involved in the activation and sensitization of TRPV1. This study tested whether the metabolic and renal effects of SA were mediated by the TRPV1 channel. Wild-type (WT) and TRPV1−/− mice were fed a Western diet (WD) for 4 months and received SA infusion (120mg/kg/day) or vehicle for the last 4 weeks of WD feeding. SA treatment significantly increased blood pressure in WD-fed TRPV1−/− mice (p < 0.05) but not in WD-fed WT mice. Similarly, SA impaired renal blood flow in TRPV1−/− mice (p < 0.05) but not in WT mice. SA improved insulin and glucose tolerance in both WT and TRPV1−/− mice on WD (all p < 0.05). In addition, SA reduced renal p65 and urinary prostaglandin E2, prostaglandin F1α, and interleukin-6 in both WT and TRPV1−/− mice (all p < 0.05). SA decreased urine noradrenaline levels, increased afferent renal nerve activity, and improved baroreflex sensitivity in WT mice (all p < 0.05) but not in TRPV1−/− mice. Importantly, SA increased serum creatinine and urine kidney injury molecule-1 levels and decreased the glomerular filtration rate in obese WT mice (all p < 0.05), and these detrimental effects were significantly exacerbated in obese TRPV1−/− mice (all p < 0.05). Lastly, SA treatment increased urine albumin levels in TRPV1−/− mice (p < 0.05) but not in WT mice. Taken together, SA-elicited metabolic benefits and anti-inflammatory effects are independent of TRPV1, while SA-induced sympathetic suppression is dependent on TRPV1 channels. SA-induced renal dysfunction is dependent on intact TRPV1 channels. These findings suggest that SA needs to be cautiously used in patients with obesity or diabetes, as SA-induced renal dysfunction may be exacerbated due to impaired TRPV1 in obese and diabetic patients.
To Ubiquitinate or Not to Ubiquitinate: TRIM17 in Cell Life and Death
Authors: Basu-Shrivastava Meenakshi , Kozoriz Alina , Desagher Solange , Lassot Iréna
Published: May 2021

TRIM17 is a member of the TRIM family, a large class of RING-containing E3 ubiquitin-ligases. It is expressed at low levels in adult tissues, except in testis and in some brain regions. However, it can be highly induced in stress conditions which makes it a putative stress sensor required for the triggering of key cellular responses. As most TRIM members, TRIM17 can act as an E3 ubiquitin-ligase and promote the degradation by the proteasome of substrates such as the antiapoptotic protein MCL1. Intriguingly, TRIM17 can also prevent the ubiquitination of other proteins and stabilize them, by binding to other TRIM proteins and inhibiting their E3 ubiquitin-ligase activity. This duality of action confers several pivotal roles to TRIM17 in crucial cellular processes such as apoptosis, autophagy or cell division, but also in pathological conditions as diverse as Parkinson’s disease or cancer. Here, in addition to recent data that endorse this duality, we review what is currently known from public databases and the literature about TRIM17 gene regulation and expression, TRIM17 protein structure and interactions, as well as its involvement in cell physiology and human disorders.
Preliminary Phytochemical Analysis and Evaluation of the Biological Activity of Leonotis nepetifolia (L.) R. Br Transformed Roots Extracts Obtained through Rhizobium rhizogenes-Mediated Transformation
Authors: Kowalczyk Tomasz , Merecz-Sadowska Anna , Rijo Patricia , Isca Vera M. S. , Picot Laurent , Wielanek Marzena , Śliwiński Tomasz , Sitarek Przemysław
Published: May 2021

According to the present knowledge, this is the first report on establishing transformed root cultures of Leonotis nepetifolia after Rhizobium rhizogenes-mediated transformation. The preliminary phytochemical analysis showed differences in the content of phenols and flavonoids in transformed and nontransformed roots. The dominant compounds in the analyzed extracts were (+)-catechin (5464 and 6808 µg/g DW), p-coumaric acid (2549 and 4907 µg/g DW), m-coumaric acid (1508 and 2048 µg/g DW) and rosmarinic acid (1844 and 2643 µg/g DW) for nontransformed (LNNR) and transformed (LNTR4) roots, respectively. Initial biological studies carried out on LNNR, and LNTR4 extracts showed a cytotoxic effect on the A549 lung, HCC1937 breast and leukemia NALM-6 cell lines, antioxidants, as well as repair and protection against DNA damage induced by H2O2 in HUVEC cells. Due to the stronger effect of the LNTR4 root extract, which can be a relatively efficient and cheap source of bioactive secondary metabolites, further biological analyses are needed to discover in detail their potentially valuable biological properties.
Segmental Evaluation of Thoracic Aortic Calcium and Their Relations with Cardiovascular Risk Factors in the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil)
Authors: Pedrosa Jesiana Ferreira , Brant Luisa Campos Caldeira , de Aquino Stephanie Alves , Ribeiro Antonio Luiz , Barreto Sandhi Maria
Published: May 2021

Thoracic aortic calcium (TAC) appears to be a subclinical marker of cardiovascular disease (CVD) and to predict cardiovascular (CV) mortality. However, studies on TAC use tomographic scans obtained for coronary artery calcium (CAC) score, which does not include the aortic arch. This study evaluates TAC prevalence in aortic arch (AAC), ascending (ATAC) and descending thoracic aorta (DTAC) and verify whether they are associated with the same CV risk factors. Cross-sectional analysis, including 2427 participants (mean age 55.6 ± 8.7; 54.1% women) of the ELSA-Brasil cohort. Nonenhanced ECG-gated tomographies were performed in 2015–2016. Multivariable logistic regression estimated the CV risk factors associated with calcium in each segment. Overall prevalence of ATAC, AAC and DTAC was, 23.1%, 62.1%, and 31.2%, respectively. About 90.4% of the individuals with TAC had AAC and only 19.5% had calcium in all segments. In the multivariable analysis, increasing age, lower levels of schooling, current smoking, higher body mass index, and hypertension remained associated with calcium in all segments. No sex or race/ethnicity differences were found in any aortic segment. Diabetes and dyslipidemia were associated with ATAC and DTAC, but not with AAC, suggesting that AAC may reflect an overlap of mechanisms that impact vascular health, including atherosclerosis.
Prolonged Indwelling Urethral Catheterization as Minimally Invasive Approach for Definitive Treatment of Posterior Urethral Valves in Unstable Premature Babies
Authors: Ceccanti Silvia , Pepino Daniela , Giancotti Antonella , Ricci Ester , Piacenti Silvia , Cozzi Denis A.
Published: May 2021

Premature newborns with posterior urethral valves (PUV) may present with medical conditions taking priority over definitive surgical care. We encountered three of such cases who underwent initial bladder decompression via transurethral catheterization and waited 2–3 weeks until they were fit enough for voiding cysto-urethrography to confirm PUV. An unexpected good urinary flow and negligible residual urine volume were documented during micturition, suggestive of valve disruption induced by insertion and prolonged duration of indwelling urethral catheter drainage. Cystoscopy documented non-obstructing remnant leaflets. Non-operative treatment may be considered as a viable alternative therapeutic option for PUV in tiny babies facing prolonged intensive care unit stay.
Effect of SnO2 Colloidal Dispersion Solution Concentration on the Quality of Perovskite Layer of Solar Cells
Authors: Song Keke , Zou Xiaoping , Zhang Huiyin , Zhang Chunqian , Cheng Jin , Liu Baoyu , Yao Yujun , Wang Xiaolan , Li Xiaotong , Wang Yifei , Ren Baokai
Published: May 2021

The electron transport layer (ETL) is critical to carrier extraction for perovskite solar cells (PSCs). Moreover, the morphology and surface condition of the ETL could influence the topography of the perovskite layer. ZnO, TiO2, and SnO2 were widely investigated as ETL materials. However, TiO2 requires a sintering process under high temperature and ZnO has the trouble of chemical instability. SnO2 possesses the advantages of low-temperature fabrication and high conductivity, which is critical to the performance of PSCs prepared under low temperature. Here, we optimized the morphology and property of SnO2 by modulating the concentration of a SnO2 colloidal dispersion solution. When adjusting the concentration of SnO2 colloidal dispersion solution to 5 wt.% (in water), SnO2 film indicated better performance and the perovskite film has a large grain size and smooth surface. Based on high efficiency (16.82%), the device keeps a low hysteresis index (0.23).
Corrosion Resistance of AISI 304 Stainless Steel Modified Both Femto- and Nanosecond Lasers
Authors: Mroczkowska Katarzyna M. , Dzienny Paulina , Budnicki Aleksander , Antończak Arkadiusz J.
Published: May 2021

This article is aimed to study the effect of laser treatment of AISI 304 stainless steel on the corrosion resistance and chemical composition of the surface layer. The samples were irradiated using two quite different laser sources: IPG Yb:glass fibre laser (τ = 230 ns, λ = 1062 nm) and Trumpf TruMicro Series 2020 fiber laser (τ = 260 fs–20 ps, λ = 1030 nm) that is, in both the long and ultra-short pulse duration regime. It allowed the observation of completely different microstructures and chemical composition of the surface layer. In this study, the morphology of the samples was accessed using both Keyence digital microscope and Olympus Lext 5000 profilometer. The corrosion resistance was examined in 3% NaCl solution using both potentiodynamic measurement and Electrochemical Impedance Spectroscopy. In order to examine the change in chemical composition of the surface layer, the X-ray photoelectron spectroscopy study was performed. Results show that the use of a long laser pulse contributes to the formation of a thin, tight, rich in chromium passive layer, which significantly improves corrosion resistance in comparison to the reference sample. Different behaviour is observed after irradiation with an ultra-short pulse duration laser.
Numerical Study of the In-Plane Seismic Response of RC Infilled Frames
Authors: Bagnoli Matteo , Grande Ernesto , Milani Gabriele
Published: May 2021

Reinforced Concrete (RC) buildings with masonry infills are a very common structural typology worldwide for civil, strategic, or productive use. Damage to infills may cause danger for human lives and strongly affects economic losses, as shown during past earthquakes. In the current literature, different approaches are available for modeling the in-plane response of infilled frames and different constitutive laws generally calibrated on experimental tests. On the contrary, few and recent studies proposed formulas that account for the main properties of infills influencing their in-plane behavior to lateral forces. This paper presents a study finalized to derive a reliable model that is able to predict the monotonic and cyclic response of RC infilled masonry frames. To this end, after a critical analysis of the available literature, the authors combine among them two models, one for the monotonic response and the other for the cyclic one, by showing their reliability with reference to different experimental cases. Then, at the end of the paper, the derived models are employed to assess the seismic vulnerability of infills throughout a proposed procedure based on the common pushover analysis approach.
Quartz Crystal Microbalance Genosensing of Brettanomyces bruxellensis Yeast in Wine Using a Rapid and Efficient Drop and Collect Protocol
Authors: Poggesi Simone , Zhou Lan , Bariani Giuliocesare Casari , Mittapalli Rakesh , Manzano Marisa , Ionescu Rodica Elena
Published: May 2021

A miniaturized quartz crystal microbalance (QCM) genosensor is proposed for sensitive and real-time detection of short ssDNA sequences (53 bp) or DNA extracted from Brettanomyces bruxellensis (Brett) yeast cells. The presence of Brett yeast causes a depreciation of the quality of aged fine wines, producing molecules of unpleasant odors and biogenic amines that are harmful to human health. More specifically, standard quartz crystal (S-QCM) and homemade 4 nm gold transmission electron microscopy (TEM)-grid patterned quartz (multi-TEM QCM) are herein proposed for biofunctionalization steps with different ssDNA sequences. By employing a rapid and efficient drop and collect protocol, the specific detection of 1 pg/µL ssDNA Brett of a short sequence and 100 ng/μL DNA of B. bruxellensis extracted from a wine sample (VR2008) is reported.
The Phylogenetics and Biogeography of the Central Asian Hawkmoths, Hyles hippophaes and H. chamyla: Can Mitogenomics and Machine Learning Bring Clarity?
Authors: Patzold Franziska , Marabuto Eduardo , Daneck Hana , O’Neill Mark A. , Kitching Ian J. , Hundsdoerfer Anna K.
Published: May 2021

The western Palaearctic species of the hawkmoth genus Hyles (Lepidoptera: Sphingidae) have long been the subject of molecular phylogenetic research. However, much less attention has been paid to the taxa inhabiting the central and eastern Palaearctic, particularly Central Asia, where almost 50% of the species diversity of the genus occurs. Yet, many taxonomic conundrums hinder a proper assessment of the true diversity in these moths. One still unresolved group of species includes Hyles hippophaes and Hyles chamyla. Despite a largely overlapping morphology and ecology, a plethora of infraspecific taxa display some unique divergent characters over a wide geographical area. In this study, we undertook a taxonomic assessment of each population and resolved this species complex using an integrative approach. A combination of new computational techniques (DAISY-II) in comparative morphology and recent advances in DNA extraction methods and sequencing of museum specimens (WISC) alongside more traditional genetic approaches allowed testing of the three main phenotypes—bienerti, chamyla and apocyni—in terms of their morphological, mitochondrial and biogeographical integrity, and to elucidate their evolutionary relationships. Our results support the existence of two closely related species, Hyles chamyla and H. hippophaes, but the former species H. apocyni (here discussed as the ecological form apocyni of H. chamyla) is best regarded as a hybrid between H. chamyla and H. h. bienerti. The results indicate that the evolutionary relationship between H. chamyla and H. hippophaes is one of admixture in the context of ongoing ecological differentiation, which has led to shared morphological characters and a blurring of the species boundaries. These results clarify the evolutionary relationships of this species complex and open future research lines, including the analysis of nuclear markers and denser sampling, particularly of H. hippophaes and H. vespertilio in western Europe.
Association of Alpha B-Crystallin Expression with Tumor Differentiation Grade in Colorectal Cancer Patients
Authors: Pagano Cristina , Navarra Giovanna , Gazzerro Patrizia , Vitale Mario , Notarnicola Maria , Caruso Maria Gabriella , Cavalcanti Elisabetta , Armentano Raffaele , Laezza Chiara , Bifulco Maurizio
Published: May 2021

Alpha B-crystallin (CRYAB, HSPB5) belongs to the small heat shock protein (HSP) family and is highly expressed in various human cancers, suggesting a crucial role in tumor progression. However, few studies have examined CRYAB expression in colorectal cancer (CRC). In the present study, we investigated the relationship between CRYAB expression and the clinicopathological features of CRC samples. We comparatively analyzed CRYAB protein expression in 111 CRC tissues and normal adjacent colonic tissue, observing that it was significantly lower in CRC tissues than in corresponding non-cancerous tissues. Moreover, immunohistochemical analysis showed a significant correlation between CRYAB expression and high histological grade G3 (p = 0.033). In summary, our results point to its possible application as a prognostic biomarker in CRC patients.
Interpolating between Positive and Completely Positive Maps: A New Hierarchy of Entangled States
Authors: Siudzińska Katarzyna , Chakraborty Sagnik , Chruściński Dariusz
Published: May 2021

A new class of positive maps is introduced. It interpolates between positive and completely positive maps. It is shown that this class gives rise to a new characterization of entangled states. Additionally, it provides a refinement of the well-known classes of entangled states characterized in terms of the Schmidt number. The analysis is illustrated with examples of qubit maps.
Diagnosis and Robust Design Optimization of SPMSM Considering Back EMF and Cogging Torque due to Static Eccentricity
Authors: Park Jin-Cheol , Park Soo-Hwan , Kim Jae-Hyun , Lee Soo-Gyung , Lee Geun-Ho , Lim Myung-Seop
Published: May 2021

Static eccentricity (SE) is frequently generated by manufacturing processes. As the nonuniformity of the air-gap length is caused by the SE, the torque ripple and cogging torque increase in the motor. This study analyzes the distorted back electromotive force (EMF) and cogging torque due to SE. Further, a motor design considering SE is performed for stable back EMF and low cogging torque. First, the SE was diagnosed and analyzed using the back EMF and cogging torque measured from the test results of the base model. In addition, the rotor position was calculated using the unbalanced back EMF due to the SE. The calculated rotor position is used when analyzing phenomena due to SE and applied to robust design. Subsequently, robust design optimization was performed to improve the unbalanced back EMF and cogging torque due to SE. Using finite element analysis (FEA) considering SE, the shape of the stator was designed based on the base model. The estimated rotor position from the base model was applied to the optimum model to confirm its robustness from SE’s effects. Finally, the base and optimum models were compared through the test results.
Core-Loss Analysis of Linear Magnetic Gears Using the Analytical Method
Authors: Lee Jeong-In , Shin Kyung-Hun , Bang Tae-Kyoung , Kim Kyong-Hwan , Hong Key-Yong , Choi Jang-Young
Published: May 2021

In this study, analysis of core-loss occurring in the magnetic flux modulation core of a linear magnetic gear and the core of each mover is presented, using an analytical method. Losses in electric machines were generally calculated and analyzed using the finite element method (FEM). However, in the case of core-loss, the exact loss value could not be calculated using FEM data. Therefore, we considered the harmonic component of the air-gap magnetic flux density waveform with the modified Steinmetz equation, and performed a more accurate core-loss analysis with magnetic behavior analysis. Thus, we performed a calculated core-loss characteristic comparison with the FEM and the modified Steinmetz equation.
Application of High-Frequency Leakage Current Model for Characterizing Failure Modes in Digital Logic Gates
Authors: Abedi Zahra , Hemmady Sameer , Antonsen Thomas , Schamiloglu Edl , Zarkesh-Ha Payman
Published: May 2021

In this paper, a predictive model is developed to characterize the impact of high-frequency electromagnetic interference (EMI) on the leakage current of CMOS integrated circuits. It is shown that the frequency dependence can be easily described by a transfer function that depends only on a few dominant parasitic elements. The developed analytical model is successfully compared against measurement data from devices fabricated using 180 nm, 130 nm, and 65 nm standard CMOS processes through TSMC. Based on the predictive model, the impact of EMI on leakage current in a CMOS inverter is reduced by increasing the frequency from 10 MHz to 4 GHz.