bluuebunny/crossref_metadata_2025 huggingface dataset:
Altered Morpho-Functional Features of Neurogenesis in Zebrafish Embryos Exposed to Non-Combustion-Derived Magnetite
Authors: Cacialli Pietro , Ricci Serena , Servetto Giulia Pia , Franceschini Valeria , Ruiz-Zepeda Francisco , Vigliaturo Ruggero
Published: June 2024

Neurogenesis is the process by which new brain cells are formed. This crucial event emerges during embryonic life and proceeds in adulthood, and it could be influenced by environmental pollution. Non-combustion-derived magnetite represents a portion of the coarse particulate matter (PM) contributing to air and water pollution in urban settings. Studies on humans have reported that magnetite and other iron oxides have significant damaging effects at a central level, where these particles accumulate and promote oxidative stress. Similarly, magnetite nanoparticles can cross the placenta and damage the embryo brain during development, but the impact on neurogenesis is still unknown. Furthermore, an abnormal Fe cation concentration in cells and tissues might promote reactive oxygen species (ROS) generation and has been associated with multiple neurodegenerative conditions. In the present study, we used zebrafish as an in vivo system to analyze the specific effects of magnetite on embryonic neurogenesis. First, we characterized magnetite using mineralogical and spectroscopic analyses. Embryos treated with magnetite at sub-lethal concentrations showed a dose–response increase in ROS in the brain, which was accompanied by a massive decrease in antioxidant genes (sod2, cat, gsr, and nrf2). In addition, a higher number of apoptotic cells was observed in embryos treated with magnetite. Next, interestingly, embryos exposed to magnetite displayed a decrease in neural staminal progenitors (nestin, sox2, and pcna markers) and a neuronal marker (elavl3). Finally, we observed significative increases in apoeb (specific microglia marker) and interleukin-1b (il1b), confirming a status of inflammation in the brain embryos treated with magnetite. Our study represents the very first in vivo evidence concerning the effects of magnetite on brain development.
Association between Reactive Oxygen Species, Transcription Factors, and Candidate Genes in Drought-Resistant Sorghum
Authors: Liu Jiao , Wang Xin , Wu Hao , Zhu Yiming , Ahmad Irshad , Dong Guichun , Zhou Guisheng , Wu Yanqing
Published: June 2024

Drought stress is one of the most severe natural disasters in terms of its frequency, length, impact intensity, and associated losses, making it a significant threat to agricultural productivity. Sorghum (Sorghum bicolor), a C4 plant, shows a wide range of morphological, physiological, and biochemical adaptations in response to drought stress, paving the way for it to endure harsh environments. In arid environments, sorghum exhibits enhanced water uptake and reduced dissipation through its morphological activity, allowing it to withstand drought stress. Sorghum exhibits physiological and biochemical resistance to drought, primarily by adjusting its osmotic potential, scavenging reactive oxygen species, and changing the activities of its antioxidant enzymes. In addition, certain sorghum genes exhibit downregulation capabilities in response to drought stress. Therefore, in the current review, we explore drought tolerance in sorghum, encompassing its morphological characteristics and physiological mechanisms and the identification and selection of its functional genes. The use of modern biotechnological and molecular biological approaches to improving sorghum resistance is critical for selecting and breeding drought-tolerant sorghum varieties.
The Anthraquinone Derivative C2 Enhances Oxaliplatin-Induced Cell Death and Triggers Autophagy via the PI3K/AKT/mTOR Pathway
Authors: Li Yuying , Yan Wei , Qin Yu , Zhang Liwei , Xiao Sheng
Published: June 2024

Chemotherapy resistance in cancer is an essential factor leading to high mortality rates. Tumor multidrug resistance arises as a result of the autophagy process. Our previous study found that compound 1-nitro-2 acyl anthraquinone-leucine (C2) exhibited excellent anti-colorectal cancer (CRC) activity involving autophagy and apoptosis-related proteins, whereas its underlying mechanism remains unclear. A notable aspect of this study is how C2 overcomes the multidrug susceptibility of HCT116/L-OHP, a colon cancer cell line that is resistant to both in vitro and in vivo oxaliplatin (trans-/-diaminocyclohexane oxalatoplatinum; L-OHP). In a xenograft tumor mouse model, we discovered that the mixture of C2 and L-OHP reversed the resistance of HCT116/L-OHP cells to L-OHP and inhibited tumor growth; furthermore, C2 down-regulated the gene expression levels of P-gp and BCRP and decreased P-gp’s drug efflux activity. It is important to note that while C2 re-sensitized the HCT116/L-OHP cells to L-OHP for apoptosis, it also triggered a protective autophagic pathway. The expression levels of cleaved caspase-3 and Beclin 1 steadily rose. Expression of PI3K, phosphorylated AKT, and mTOR were decreased, while p53 increased. We demonstrated that the anthraquinone derivative C2 acts as an L-OHP sensitizer and reverses resistance to L-OHP in HCT116/L-OHP cells. It suggests that C2 can induce autophagy in HCT116/L-OHP cells by mediating p53 and the PI3K/AKT/mTOR signaling pathway.
Immunotherapy Based on Immune Checkpoint Molecules and Immune Checkpoint Inhibitors in Gastric Cancer–Narrative Review
Authors: Poniewierska-Baran Agata , Sobolak Karolina , Niedźwiedzka-Rystwej Paulina , Plewa Paulina , Pawlik Andrzej
Published: June 2024

Due to its rapid progression to advanced stages and highly metastatic properties, gastric cancer (GC) is one of the most aggressive malignancies and the fourth leading cause of cancer-related deaths worldwide. The metastatic process includes local invasion, metastasis initiation, migration with colonisation at distant sites, and evasion of the immune response. Tumour growth involves the activation of inhibitory signals associated with the immune response, also known as immune checkpoints, including PD-1/PD-L1 (programmed death 1/programmed death ligand 1), CTLA-4 (cytotoxic T cell antigen 4), TIGIT (T cell immunoreceptor with Ig and ITIM domains), and others. Immune checkpoint molecules (ICPMs) are proteins that modulate the innate and adaptive immune responses. While their expression is prominent on immune cells, mainly antigen-presenting cells (APC) and other types of cells, they are also expressed on tumour cells. The engagement of the receptor by the ligand is crucial for inhibiting or stimulating the immune cell, which is an extremely important aspect of cancer immunotherapy. This narrative review explores immunotherapy, focusing on ICPMs and immune checkpoint inhibitors in GC. We also summarise the current clinical trials that are evaluating ICPMs as a target for GC treatment.
MicroRNA as Sepsis Biomarkers: A Comprehensive Review
Authors: Bindayna Khalid
Published: June 2024

Sepsis, a life-threatening condition caused by the body’s dysregulated response to infection, presents a significant challenge in clinical management. Timely and accurate diagnosis is paramount for initiating appropriate interventions and improving patient outcomes. In recent years, there has been growing interest in identifying biomarkers that can aid in the early detection and prognostication of sepsis. MicroRNAs (miRNAs) have emerged as potential biomarkers for sepsis due to their involvement in the regulation of gene expression and their stability in various biological fluids, including blood. MiRNAs are small non-coding RNA molecules that play crucial roles in post-transcriptional gene regulation by binding to target messenger RNAs (mRNAs), leading to mRNA degradation or translational repression. The diagnostic and prognostic potential of miRNAs in sepsis stems from their ability to serve as sensitive and specific biomarkers reflective of the underlying pathophysiological processes. Compared to traditional biomarkers such as C-reactive protein (CRP) and procalcitonin (PCT), miRNAs offer several advantages, including their early and sustained elevation during sepsis, as well as their stability in stored samples, making them attractive candidates for clinical use. However, despite their promise, the clinical translation of miRNAs as sepsis biomarkers faces several challenges. These include the need for standardized sample collection and processing methods, the identification of optimal miRNA panels or signatures for differentiating sepsis from other inflammatory conditions, and the validation of findings across diverse patient populations and clinical settings. In conclusion, miRNAs hold great promise as diagnostic and prognostic biomarkers for sepsis, offering insights into the underlying molecular mechanisms and potential therapeutic targets. However, further research is needed to overcome existing challenges and realize the full clinical utility of miRNAs in improving sepsis outcomes.
Mechanisms Underlying the Rarity of Skeletal Muscle Cancers
Authors: Kump David S.
Published: June 2024

Skeletal muscle (SKM), despite comprising ~40% of body mass, rarely manifests cancer. This review explores the mechanisms that help to explain this rarity, including unique SKM architecture and function, which prohibits the development of new cancer as well as negates potential metastasis to SKM. SKM also presents a unique immune environment that may magnify the anti-tumorigenic effect. Moreover, the SKM microenvironment manifests characteristics such as decreased extracellular matrix stiffness and altered lactic acid, pH, and oxygen levels that may interfere with tumor development. SKM also secretes anti-tumorigenic myokines and other molecules. Collectively, these mechanisms help account for the rarity of SKM cancer.
Advancements in Heavy Metal Stabilization: A Comparative Study on Zinc Immobilization in Glass-Portland Cement Binders
Authors: Bouchikhi Abdelhadi , Safhi Amine el Mahdi , Maherzi Walid , Mamindy-Pajany Yannick , Kunther Wolfgang , Benzerzour Mahfoud , Abriak Nor-Edine
Published: June 2024

Recent literature has exhibited a growing interest in the utilization of ground glass powder (GP) as a supplementary cementitious material (SCM). Yet, the application of SCMs in stabilizing heavy metallic and metalloid elements remains underexplored. This research zeroes in on zinc stabilization using a binder amalgam of GP and ordinary Portland cement (OPC). This study juxtaposes the stability of zinc in a recomposed binder consisting of 30% GP and 70% OPC (denoted as 30GP-M) against a reference binder of 100% CEM I 52.5 N (labeled reference mortar, RM) across curing intervals of 1, 28, and 90 days. Remarkably, the findings indicate a heightened kinetic immobilization of Zn at 90 days in the presence of GP—surging up to 40% in contrast to RM. Advanced microstructural analyses delineate the stabilization locales for Zn, including on the periphery of hydrated C3S particles (Zn–C3S), within GP-reactive sites (Si*–O–Zn), and amid C–S–H gel structures, i.e., (C/Zn)–S–H. A matrix with 30% GP bolsters the hydration process of C3S vis-à-vis the RM matrix. Probing deeper, the microstructural characterization underscores GP’s prowess in Zn immobilization, particularly at the interaction zone with the paste. In the Zn milieu, it was discerning a transmutation—some products born from the GP–Portlandite reaction morph into GP–calcium–zincate.
Depression in the Perinatal Period: Course and Outcome of Depression in the Period from the Last Trimester of Pregnancy to One Year after Delivery in Primiparous Mothers
Authors: Zikic Olivera , Stojanov Jelena , Kostic Jelena , Nikolic Gordana , Tosic Golubovic Suzana , Simonovic Maja , Djordjevic Vladimir , Binic Iva
Published: June 2024

Background and Objectives: One of the most significant psychiatric problems in women is depression related to the perinatal period. Our study aims to determine the frequency and course of depressive symptomatology in the perinatal period with particular reference to objective rate and outcome of postpartum depression. Materials and Methods: One hundred and eighty-eight pregnant/postnatal women were included in a prospective, longitudinal, observational study during which the depressive symptomatology was estimated at the third trimester of pregnancy, and the first, sixth, and twelfth month‚ postpartum. All participants completed a semi-structured sociodemographic questionnaire constructed for research purposes, the Edinburgh Postnatal Depression Scale, Toronto Alexithymia Scale, Beck Anxiety Inventory, and The Mood Disorder Questionnaire at each time point. Postpartum depression diagnosis was confirmed by a trained and certified psychiatrist with long-standing experience. For a better understanding of the trajectory of depressive symptomatology and genuine postpartum depression, we classified depression into those with new-onset and those left over from the previous observation period. Results: In general, 48.9% of participants in the study were depressed at some point during the investigation. A total of 10.6% of women were depressed in the third trimester. The highest percentage of new-onset depression (25%) was in the first month after giving birth and was maintained for up to six months, after which the appearance was sporadic. Most of the postpartum depression resolved in the period from the first month to the sixth month after childbirth (20.7%). The episodes mainly had characteristics of unipolar depression. Conclusions: Our results imply that a new onset of depression is most intensive during the first six months, and after that, it is sporadic. Further studies are needed to explore whether all depressive symptomatology in the postnatal period is the same, or perhaps postpartum depression, classified in this way, has specific characteristics, etiology, and consequently different treatment and preventive options.
Remimazolam-Induced Anaphylaxis and Cardiovascular Collapse: A Narrative Systematic Review of Eleven Cases
Authors: Lee Jaemoon , Kim Seong-Hyop
Published: June 2024

Background and Objectives: Remimazolam, a novel benzodiazepine, is used for procedural sedation and general anesthesia due to its rapid onset and short duration of action. However, remimazolam-induced anaphylaxis (RIA) is a rare but severe complication. This study aimed to analyze RIA characteristics, focusing on cardiovascular collapse, and provide guidelines for safe remimazolam use. Methods: This study conducted a systematic review using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses 2020 guidelines. Research articles retrieved from PubMed on 26 May 2023, using the keywords ‘remimazolam AND anaphylaxis’ were evaluated based on the inclusion criteria of being written in English and aligning with the World Allergy Organization criteria for anaphylaxis, while studies not meeting these criteria were excluded. All published articles up to the search date were included without any date restrictions. The review analyzed factors such as age, sex, type of anesthesia, remimazolam dose (bolus/continuous), allergic symptoms and sign, epinephrine use, serum tryptase levels, and skin prick tests. Results: Among eleven cases, the mean age was 55.6 ± 19.6 years, with 81.8% male. Hypotension (81.8%) was the most common symptom, followed by bradycardia (54.5%) and desaturation (36.4%). Two patients experienced cardiac arrest. Serum tryptase levels confirmed anaphylaxis in ten cases. Epinephrine was the primary treatment, with intravenous doses ranging from 0.1 mg to 0.3 mg. Conclusions: Vigilance is crucial when administering remimazolam, adhering to recommended dosages, and promptly treating RIA with epinephrine. Further research is needed to understand the risk factors and refine the management strategies. Guidelines for safe remimazolam use are proposed.
A Novel Compliant Connection Mechanism with Thermal Distortion Self-Elimination Function
Authors: Huang Yunyang , Liao Zhanchen , Lin Zhihang , Feng Fahui , Tang Hui
Published: June 2024

As a novel technology for fabricating large-screen OLED devices, OLED inkjet printing places extreme demands on the positioning accuracy of inkjet printing platforms. However, thermal deformation of the connection mechanism often reduces the printing precision of OLED printing equipment, significantly impacting overall print quality. This study introduces a compliant connection mechanism that achieves precise positioning of the inkjet printing platform and can self-eliminate thermal distortion. The design of the mechanism’s core component is based on the Freedom and Constraint Topology (FACT) principle. This component is constructed from three distinct compliant sections arranged in series, collectively providing three degrees of freedom. Furthermore, the resistance to deformation caused by gravity and other external forces was evaluated by analyzing both vertical and horizontal stiffness. To validate the mechanism’s thermal distortion elimination and gravity resistance capabilities, finite element analysis (FEA) was carried out. The results demonstrate that the mechanism effectively reduces the maximum deformation of the platform by approximately 46% and the average deformation across the entire platform by approximately 59%. These findings confirm that the mechanism has potential in high-precision positioning tasks that need to mitigate thermal distortion.
Solid Lipid Nanoparticles Based on Babassu Oil and Copaiba Oleoresin: A Promising Approach for Prostate Cancer Therapy
Authors: da Silva Michael Jackson Ferreira , Rodrigues Alisson Mendes , Costa Maria Célia Pires , Camara Adriana Leandro , Cabral Lucio Mendes , Ricci Junior Eduardo , Vanzan Daniel Figueiredo , Matos Ana Paula dos Santos , da Silva Honorio Thiago , Borges Antonio Carlos Romão
Published: June 2024

Solid lipid nanoparticles (SLNs) represent promising nanostructures for drug delivery systems. This study successfully synthesized SLNs containing different proportions of babassu oil (BBS) and copaiba oleoresin (COPA) via the emulsification–ultrasonication method. Before SLN synthesis, the identification and quantification of methyl esters, such as lauric acid and β-caryophyllene, were performed via GC-MS analysis. These methyl esters were used as chemical markers and assisted in encapsulation efficiency experiments. A 22 factorial design with a center point was employed to assess the impact of stearic acid and Tween 80 on particle hydrodynamic diameter (HD) and polydispersity index (PDI). Additionally, the effects of temperature (8 ± 0.5 °C and 25 ± 1.0 °C) and time (0, 7, 15, 30, 40, and 60 days) on HD and PDI values were investigated. Zeta potential (ZP) measurements were utilized to evaluate nanoparticle stability, while transmission electron microscopy provided insights into the morphology and nanometric dimensions of the SLNs. The in vitro cytotoxic activity of the SLNs (10 µg/mL, 30 µg/mL, 40 µg/mL, and 80 µg/mL) was evaluated using the MTT assay with PC-3 and DU-145 prostate cancer cell lines. Results demonstrated that SLNs containing BBS and COPA in a 1:1 ratio exhibited a promising cytotoxic effect against prostate cancer cells, with a percentage of viable cells of 68.5% for PC-3 at a concentration of 30 µg/mL and 48% for DU-145 at a concentration of 80 µg/mL. These findings underscore the potential therapeutic applications of SLNs loaded with BBS and COPA for prostate cancer treatment.
Moderate-Intensity Exercise Enhances Mitochondrial Biogenesis Markers in the Skeletal Muscle of a Mouse Model Affected by Diet-Induced Obesity
Authors: Jun Lauren , Knight Emily , Broderick Tom L. , Al-Nakkash Layla , Tobin Brielle , Geetha Thangiah , Babu Jeganathan Ramesh
Published: June 2024

Skeletal muscle is composed of bundles of muscle fibers with distinctive characteristics. Oxidative muscle fiber types contain higher mitochondrial content, relying primarily on oxidative phosphorylation for ATP generation. Notably, as a result of obesity, or following prolonged exposure to a high-fat diet, skeletal muscle undergoes a shift in fiber type toward a glycolytic type. Mitochondria are highly dynamic organelles, constantly undergoing mitochondrial biogenesis and dynamic processes. Our study aims to explore the impact of obesity on skeletal muscle mitochondrial biogenesis and dynamics and also ascertain whether the skeletal muscle fiber type shift occurs from the aberrant mitochondrial machinery. Furthermore, we investigated the impact of exercise in preserving the oxidative muscle fiber types despite obesity. Mice were subjected to a normal standard chow and water or high-fat diet with sugar water (HFS) with or without exercise training. After 12 weeks of treatment, the HFS diet resulted in a noteworthy reduction in the markers of mitochondrial content, which was recovered by exercise training. Furthermore, higher mitochondrial biogenesis markers were observed in the exercised group with a subsequent increase in the mitochondrial fission marker. In conclusion, these findings imply a beneficial impact of moderate-intensity exercise on the preservation of oxidative capacity in the muscle of obese mouse models.
Chemical Behavior and Bioactive Properties of Spinorphin Conjugated to 5,5′-Dimethyl- and 5,5′-Diphenylhydantoin Analogs
Authors: Georgieva Stela , Todorov Petar , Tchekalarova Jana , Subaer Subaer , Peneva Petia , Chakarov Kalin , Hartati Hartati , Faika Sitti
Published: June 2024

The discovery of new peptides and their derivatives is an outcome of ongoing efforts to identify a peptide with significant biological activity for effective usage as a possible therapeutic agent. Spinorphin peptides have been documented to exhibit numerous applications and features. In this study, biologically active peptide derivatives based on novel peptide analogues of spinorphin conjugated with 5,5′-dimethyl (Dm) and 5,5′-diphenyl (Ph) hydantoin derivatives have been successfully synthesized and characterized. Scanning electron microscopy (SEM) and spectral methods such as UV-Vis, FT-IR (Fourier Transform Infrared Spectroscopy), CD (Circular Dichroism), and fluorimetry were used to characterize the microstructure of the resulting compounds. The results revealed changes in peptide morphology as a result of the restructuring of the aminoacidic sequences and aromatic bonds, which is related to the formation of intermolecular hydrogen bonds between tyrosyl groups and the hydantoin moiety. Electrochemical and fluorescence approaches were used to determine some physicochemical parameters related to the biological behavior of the compounds. The biological properties of the spinorphin derivatives were evaluated in vivo for anticonvulsant activity against the psychomotor seizures at different doses of the studied peptides. Both spinorphin analog peptides with Ph and Dm groups showed activity against all three phases of the seizure in the intravenous Pentylenetetrazole Seizure (ivPTZ) test. This suggests that hydantoin residues do not play a crucial role in the structure of spinorphin compounds and in determining the potency to raise the seizure threshold. On the other hand, analogs with a phenytoin residue are active against the drug-resistant epilepsy test (6-Hz test). In addition, bioactivity analyses revealed that the new peptide analogues have the potential to be used as antimicrobial and antioxidant compounds. These findings suggest promising avenues for further research that may lead to the development of alternative medicines or applications in various fields beyond epilepsy treatment.
Evaluation of Novel Nasal Mucoadhesive Nanoformulations Containing Lipid-Soluble EGCG for Long COVID Treatment
Authors: Frank Nicolette , Dickinson Douglas , Lovett Garrison , Liu Yutao , Yu Hongfang , Cai Jingwen , Yao Bo , Jiang Xiaocui , Hsu Stephen
Published: June 2024

Following recovery from the acute infection stage of the SARS-CoV-2 virus (COVID-19), survivors can experience a wide range of persistent Post-Acute Sequelae of COVID-19 (PASC), also referred to as long COVID. According to the US National Research Action Plan on Long COVID 2022, up to 23.7 million Americans suffer from long COVID, and approximately one million workers may be out of the workforce each day due to these symptoms, leading to a USD 50 billion annual loss of salary. Neurological symptoms associated with long COVID result from persistent infection with SARS-CoV-2 in the nasal neuroepithelial cells, leading to inflammation in the central nervous system (CNS). As of today, there is no evidence that vaccines or medications can clear the persistent viral infection in olfactory mucosa. Recently published clinical data demonstrate that only 5% of long COVID anosmia patients have fully recovered during the past 2 years, and 10.4% of COVID patients are still symptomatic 18 months post-infection. Our group demonstrated that epigallocatechin-3-gallate-monopalmitate (EC16m) nanoformulations possess strong antiviral activity against human coronavirus, suggesting that this green-tea-derived compound in nanoparticle formulations could be developed as an intranasally delivered new drug targeting the persistent SARS-CoV-2 infection, as well as inflammation and oxidative stress in the CNS, leading to restoration of neurologic functions. The objective of the current study was to evaluate the mucociliary safety of the EC16m nasal nanoformulations and their efficacy against human coronavirus. Methods: Nanoparticle size and Zeta potential were measured using the ZetaView Nanoparticle Tracking Analysis system; mucociliary safety was determined using the MucilAir human nasal model; contact antiviral activity and post-infection inhibition against the OC43 viral strain were assessed by the TCID50 assay for cytopathic effect on MRC-5 cells. Results: The saline-based EC16 mucoadhesive nanoformulations containing 0.005 to 0.02% w/v EC16m have no significant difference compared to saline (0.9% NaCl) with respect to tissue integrity, cytotoxicity, and cilia beat frequency. A 5 min contact resulted in 99.9% inactivation of β-coronavirus OC43. OC43 viral replication was inhibited by >90% after infected MRC-5 cells were treated with the formulations. Conclusion: The saline-based novel EC16m mucoadhesive nasal nanoformulations rapidly inactivated human coronavirus with mucociliary safety properties comparable to saline, a solution widely used for nasal applications.
Therapeutic Drug Monitoring and Pharmacokinetics-Based Individualization of Drug Therapy
Authors: Karvaly Gellert Balazs , Vásárhelyi Barna
Published: June 2024

The philosophy, practice, and clinical impact of therapeutic drug monitoring (TDM) has changed profoundly with the appearance of widely available and, in a technical sense, commonly applicable modeling, simulation, and dosing software tools in the past decade [...]
The Impact of Trauma on Addiction Workers: An Exploration of Vicarious Trauma and Vicarious Post-traumatic Growth
Authors: Annunziata Kristine Nicki , Naraindas Akansha Mahesh , Donohue Gráinne
Published: June 2024

Addiction workers play a crucial role in addressing the complex interplay between trauma and addiction, often navigating empathic connections with clients who have trauma histories. This study delves into the phenomena of vicarious trauma (VT) and vicarious post-traumatic growth (VPG) among addiction workers, exploring (counter)transference dynamics and the trauma–addiction nexus. Thematic analysis was conducted on narratives provided by six experienced addiction workers (mean age = 33 years, SD ≈ 5.86), comprising 33.33% men and 66.67% women. The analysis identified key themes including boundary dilemmas, therapeutic victories, defensive responses, and potential risk factors. The study highlights the detrimental effects of trauma on addiction workers while also revealing coping mechanisms and avenues for personal growth. Understanding the impact of trauma on addiction workers is vital for developing effective support strategies. By acknowledging both the risks of vicarious trauma and opportunities for vicarious post-traumatic growth, organizations can better support addiction workers and improve client care.
Analysis of the Effect of Base Station Motion on Underwater Handovers for Base-Station-Based Underwater Wireless Acoustic Networks
Authors: Yun Changho , Kwon Yong-Ju
Published: June 2024

In base-station-based underwater wireless acoustic networks (B-UWANs), effective handover mechanisms are necessary to ensure seamless data services for mobile nodes such as autonomous underwater vehicles (AUVs). Unlike terrestrial base stations (BSs), moored buoy BSs in B-UWANs experience motion responses due to wave loads under environmental conditions, posing unique challenges to the handover process. This study examines how BS motion affects handover decision errors, which arise when AUVs incorrectly initiate handovers to unintended BSs due to BS motion. By utilizing the AUV–BS distance as a handover triggering parameter, our analysis reveals a significant increase in decision errors within the overlapping regions when both the current and target BSs are in motion, especially when moving in the same direction. In addition, these errors intensify with the magnitude of BS motion and are exacerbated by smaller BS network radii. Based on these simulation results, we present an analytical framework that not only measures the influence of BS motion on the AUV–BS distance but also provides strategic insights for refining underwater handover protocols, thereby enhancing operational reliability and service continuity in B-UWANs.
Terahertz Sensing of L-Valine and L-Phenylalanine Solutions
Authors: Shu Jingyi , Zhou Xinli , Hao Jixuan , Zhao Haochen , An Mingming , Zhang Yichen , Zhao Guozhong
Published: June 2024

To detect and differentiate two essential amino acids (L-Valine and L-Phenylalanine) in the human body, a novel asymmetrically folded dual-aperture metal ring terahertz metasurface sensor was designed. A solvent mixture of water and glycerol with a volume ratio of 2:8 was proposed to reduce the absorption of terahertz waves by reducing the water content. A sample chamber with a controlled liquid thickness of 15 μm was fabricated. And a terahertz time-domain spectroscopy (THz-TDS) system, which is capable of horizontally positioning the samples, was assembled. The results of the sensing test revealed that as the concentration of valine solution varied from 0 to 20 mmol/L, the sensing resonance peak shifted from 1.39 THz to 1.58 THz with a concentration sensitivity of 9.98 GHz/mmol∗L−1. The resonance peak shift phenomenon in phenylalanine solution was less apparent. It is assumed that the coupling enhancement between the absorption peak position of solutes in the solution and the sensing peak position amplified the terahertz localized electric field resonance, which resulted in the increase in frequency shift. Therefore, it could be shown that the sensor has capabilities in performing the marker sensing detection of L-Valine.
ESG and the Cost of Debt: Role of Media Coverage
Authors: Rong Xiyu , Kim Myung-In
Published: June 2024

This study delves into the interplay between the Environmental, Social, and Governance (ESG) ratings and the debt costs incurred by Korean-listed companies, highlighting their pivotal significance in today’s corporate ecosystem. Our primary focus is to explore how the extent of media coverage moderates this relationship, thereby shedding light on the pivotal role that public scrutiny plays in shaping a company’s financial outcomes. Utilizing the Ordinary Least Squares (OLS) regression model, we rigorously control for industry and year effects, as well as firm-specific variations. Additionally, we conduct a series of supplementary analyses and robust tests to further strengthen the credibility of our findings. Our empirical analysis reveals that firms with poor ESG ratings, indicating corporate social irresponsibility, incur higher debt costs in the subsequent period. Notably, this adverse financial impact is significantly alleviated for companies that enjoy higher media coverage. This notable discovery underscores the potential of media scrutiny to reduce the financial burden imposed by inadequate ESG performance. Our results suggest that companies, especially those with limited media attention, should prioritize enhancing their ESG performance to mitigate potential financial implications. Overall, our research contributes to a more nuanced understanding of the intersection between corporate social responsibility, media coverage, and financial performance.
Analysis of Changes in In-Store and Online Shopping Frequencies Due to the COVID-19 Pandemic: A Case Study from Bahrain
Authors: Algherbal Eman A. , Hijazi Hussam I. , Al-Ahmadi Hassan M. , Abdullah Muhammad
Published: June 2024

Online shopping (e-shopping) has been growing steadily in recent years; however, the COVID-19 pandemic resulted in a sudden increase in this growth. This study compares the in-store shopping and e-shopping frequencies within three distinct periods, i.e., before, during, and after the COVID-19 pandemic. It further investigates the frequencies and determinants of e-shopping across the three periods. The data on the in-store shopping and e-shopping frequencies for four different product categories, i.e., grocery, household essentials, electronics, and clothes, were collected through an online questionnaire in Bahrain, resulting in a total of 401 valid responses. Wilcoxon signed-rank tests were conducted to compare the frequencies of in-store shopping and e-shopping within the three periods as well as the frequencies of e-shopping across the three periods. The effects of the determinants of e-shopping were evaluated using chi-square tests. The results revealed that e-shopping experienced a temporary surge during the COVID-19 pandemic, returning to pre-pandemic levels afterward. The frequency of e-shopping varied across product categories and periods, and e-shopping during the pandemic was higher than before. However, no significant difference was found between the periods during and after the pandemic. This study provides insights for urban and transport planners regarding the frequencies and determinants of e-shopping behavior in the context of pandemics.
Enhanced Recombinant Protein Expression in Insect Cells by Natural and Recombinant Components of Lepidoptera Hemolymph
Authors: López-Vidal Javier , Martínez-Pulgarín Susana , Martínez-Alonso Diego , Cid Miguel , Escribano José M.
Published: June 2024

Prior research has established the anti-apoptotic effects in insect cell cultures of Bombyx mori (B. mori) hemolymph, as well as the heightened production yields of recombinant proteins facilitated by baculovirus vectors in insect cells cultivated in media supplemented with this hemolymph. In this study, we investigated the hemolymph of another Lepidoptera species, Trichoplusia ni (T. ni), and observed similar beneficial effects in insect cells cultivated in media supplemented with this natural substance. We observed enhancements in both production yield (approximately 1.5 times higher) and late-stage cell viabilities post-infection (30–40% higher). Storage-protein 2 from B. mori (SP2Bm) has previously been identified as one of the abundant hemolymph proteins potentially responsible for the beneficial effects observed after the use of B. mori hemolymph-supplemented cell culture media. By employing a dual baculovirus vector that co-expresses the SP2Bm protein alongside the GFP protein, we achieved a threefold increase in reporter protein production compared to a baculovirus vector expressing GFP alone. This study underscores the potential of hemolymph proteins sourced from various Lepidoptera species as biotechnological tools to augment baculovirus vector productivities, whether utilized as natural supplements in cell culture media or as hemolymph-derived recombinant proteins co-expressed by baculovirus vectors.
Analysis of Water Temperature Variations in the Yangtze River’s Upper and Middle Reaches in the Context of Cascade Hydropower Development
Authors: Wang Zhangpeng , Ma Jun , Yu Shengde , Xu Yaqian , Tao Zeyi , Zhang Jiaqi , Xiao Ran , Wei Hao , Liu Defu
Published: June 2024

The establishment and operation of cascade reservoirs in the mainstream of the upper and middle reaches of the Yangtze River have changed the river’s thermal regimes. This study analyzed the correlation between water temperature and its influencing factors and employed various evaluation indexes—including ∆T (the temperature-increasing index, °C/100 km), IEC (the extreme fluctuation index), IBD (the baseline deviation index), and IPO (the phase offset time index). The aim was to uncover the variation characteristics and influencing factors of water temperature and quantify the impact of cascade reservoir construction on annual and seasonal water temperature rhythms. Our findings show that the construction and operation of cascade reservoirs weaken the synchronization of water temperature and air temperature downstream. The construction and operation of cascade reservoirs in the middle and lower reaches of the Jinsha River led to obvious homogenization, baseline deviation, and lagging effects on water temperature downstream, which intensified with the increase in storage capacity. These effects were more pronounced in colder months compared to warmer months. Additionally, the influence of tributaries and water–air heat exchange on these effects is alleviated to different degrees. These results are significant for assessing river ecological health in the context of cascade hydropower development.
Landscape Drivers of Floods Genesis (Case Study: Mayo Mizao Peri-Urban Watershed in Far North Cameroon)
Authors: Bouba Lucas , Ayral Pierre-Alain , Sauvagnargues Sophie
Published: June 2024

Landscape has significant effects on hydrological processes in a watershed. In the Sudano-Sahelian area, watersheds are subjected to a quick change in landscape patterns due to the human footprint, and the exact role of the actual landscape features in the modification of the hydrological process remains elusive. This study tends to assess the effects of landscape on the genesis of the runoff in the Mayo Mizao watershed. To achieve this goal, 62 infiltration tests were performed at different points and depths (5 cm and 20 cm) using the double-ring method and the Porchet method. The results show that the combination of many factors (soil type, land use, and farming practices) can guide the hydraulic conductivity behavior of soils. For example, at 5 cm depths, clayey-evolved soils, such as vertisols and halomorphic soils, inhibit infiltration, as opposed to non-evolved mineral soils, such as lithosols and clayey-sandy soils. However, at 20 cm depths, gray soils with halomorphic tendencies followed by vertisols have a low sensitivity to infiltration, as opposed to soils derived from loose materials and halomorphic soils. For a given soil type, rainfed crops are the primary land use that runs against infiltration. However, the effect of tillage varies according to the soil type. Finally, given the extent of vertisols and halomorphic soils in the Far North region of Cameroon in general, and in the Mayo Mizao watershed in particular, and regarding the increase in cultivated areas, a probable reduction in the infiltration capacity of soils in this region is to be expected in the medium term. The results of this study can be used as a basis for land-use planning and sustainable watershed management in semi-arid tropical zones.
Características epidemiológicas y estado nutricional de convalecientes de COVID-19 que estuvieron en ingreso domiciliario en Arroyo Naranjo, La Habana, Cuba
Authors: Cartaya Quintero Rocio , López Cancio Nathalí
Published: June 2024

La alimentación tiene un impacto importante sobre el sistema inmunitario.La evolución de los pacientes depende, entre otros factores, de su estadonutricional y de la concomitancia de otras patologías también asociadascon la calidad de la dieta. La presente investigación tuvo como objetivoevaluar el estado nutricional de pacientes convalecientes de COVID-19que estuvieron bajo ingreso domiciliario. El estudio incluyó 30 pacientes del consultorio del médico y de la familia 29, Arroyo Naranjo, LaHabana. Se aplicó un interrogatorio breve, la Herramienta de Detecciónde la Malnutrición y se midieron el peso, estatura y circunferencia de lacintura. Los principales síntomas de la enfermedad fueron hipertensiónarterial, trastornos sensoriales y malestar general. Solo el 30 % de lospacientes presentó riesgo medio de malnutrición, sin embargo, seidentificó la persistencia de náuseas e inapetencia, lo que debería implicarel reforzamiento de la vigilancia nutricional. Hubo alta incidencia delsobrepeso y una relación directa, aunque débil entre la índice cintura/talla y el índice de masa corporal. La existencia y/o la probabilidad de padecer enfermedades crónicas no transmisibles, los síntomas persistentes que han limitado la ingesta alimentaria y el sobrepeso son factores que pudieran retardar la recuperación post-COVID de los pacientes estudiados.
Respuesta inmune post infección por SARS-CoV-2 en usuarios procedentes de la costa ecuatoriana
Authors: Mero Velásquez Juan Carlos , Arteaga Quiroz Miguel Àngel
Published: June 2024

El síndrome respiratorio agudo severo causado por la enfermedad por coronavirus 2019 se caracteriza por una respuesta inmune que produce anticuerpos en más del 90 % de las personas infectadas una semana después del inicio de los síntomas, los cuales suelen persistir por un período mínimo de 3 meses, aunque su duración se cuestiona. Con el objetivo de evaluar la duración de la respuesta inmune en pacientes con infección por SARS-CoV-2, se llevó a cabo un estudio descriptivo, longitudinal, retrospectivo y cuantitativo en el Laboratorio Clínico e Histopatológico HISTOLAB. La población de estudio estuvo conformada por 96 pacientes con diagnóstico de SARS-CoV-2 y con al menos 2 determinaciones de anticuerpos IgG e IgM contra COVID-19 mediante ensayo de quimioluminiscencia. La edad promedio fue de 52,20 años, con predominio del género masculino (53.13%). En la segunda determinación, el 72.92% presentó títulos de anticuerpos IgG > 51UI. Solo el 1.04% de los hombres mayores de 65 años mantuvo títulos de anticuerpos IgG > 100 UI por más de 60 días, lo que sugiere una menor inmunidad adaptativa en este grupo de edad.
Hammering nails with a screwdriver: how GPs perceive video consultations
Authors: Wanderås Magnus Repstad , Abildsnes Eirik , Thygesen Elin , Martinez Santiago Gil
Published: June 2024

Background

Early in the COVID-19 pandemic, the use of video consultation (VC) expanded considerably, with GPs indicating high satisfaction with it. However, use of VC declined as lockdown measures were eased.

Aim

To explore reasons why VC use has declined in Norwegian general practice since the start of the pandemic by investigating GPs’ experiences with VC and their attitudes towards it in a post-pandemic setting.

Design & setting

Qualitative study using semi-structured interviews with 13 GPs in southern Norway between May 2022 and March 2023.

Method

Data analysis was conducted by applying the six steps of Braun and Clarke’s reflexive thematic analysis.

Results

Although the implementation of VCs was unplanned, most participants were able to use this modality without much problem. Several GPs initially envisioned long-term VC use. However, despite certain positives, VCs were largely sidelined in favour of face-to-face and telephone consultations, owing to their practicality and VC’s limited usefulness when considering the extra effort required. Nonetheless, GPs recognised ways of using VC that might exploit its strengths, but they highlighted how its sustained use would require them to replace other consultation modalities. They also identified extrinsic factors that might lead to the increased use of VC, including improved VC technology and patient demand.

Conclusion

Although VC is now part of many GPs’ consultation toolboxes, its perceived relative lack of usefulness and extra effort compared with other remote consultation modalities mean that most GPs have chosen to abandon it as a routine consultation modality.
Caracterização dos aspectos ecológicos das espécies listadas no Decreto nº 785 de 18 de janeiro de 2021, do Estado de Mato Grosso
Authors: Beatriz do Nascimento Marques Ana , Ribeiro Amorim Mariane , Eloisa de Freitas Milani Jaçanan
Published: June 2024

A abundância de campos e forrageiras nativas do Pantanal favorece o desenvolvimento da pecuária extensiva, principal atividade econômica na região. No entanto o exercício dessa atividade é prejudicado pela redução do suporte das pastagens nativas, por meio da invasão de espécies dominantes. Visando regulamentar o manejo para restauração das paisagens naturais, a Secretaria de Estado de Meio Ambiente de Mato Grosso, publicou o Decreto nº 785/2021, que dispõe sobre as atividades de restauração das formações campestres no pantanal Mato-grossense. Ao observar lacunas existentes na legislação vigente, tal como a falta de informações ecológicas sobre as espécies listadas, manejo e métodos de controle, bem como a possibilidade de avaliar os aspectos sócio ambientais, este trabalho tem como objetivo descrever as características ecológicas das espécies arbóreas e arbustivas listadas no Decreto nº 785/2021, buscando fornecer informações que possam complementar a legislação vigente bem como os termos de referência que orientam os responsáveis técnicos quanto ao seu manejo e controle das espécies nos campos nativos. Verificou-se que a rápida disseminação das espécies contidas na legislação está associada a características intrínsecas como a sua propagação e dispersão de sementes, além disso foi possível elencar possíveis uso econômico. As informações obtidas podem ser acrescidas nos termos de referências disponíveis, como orientação para realização de boas práticas no manejo para restauração de formações campestres nativos.
Temporal evolution of deforestation in the Xingu River Watershed between 2008 to 2020
Authors: Henrique Cordeiro Ramalho Antonio , Dalfiôr Maffioletti Fernanda , Duarte Biazatti Leonardo , Severo Carvalho Gabriel , Antoni Pinheiro da Silva Marcos Antoni , Trajano da Luz Kassiel , Pereira Amorim Janismara , Teixeira Neto Eduardo , Matos dos Santos Marcello
Published: June 2024

Anthropic activities that alter land cover patterns in Watersheds, such as deforestation, affect the food and sanitary security of the population. Thus, the objective of this study was to develop a quantitative analysis of the temporal evolution of deforestation in the Xingu River Watershed between the years 2008 and 2020. To achieve this, the methodology was organized into the following steps: Database construction (Xingu River Watershed boundaries, deforested areas, and Land Use and Land Cover); Data preprocessing; Analysis of the temporal evolution of deforestation; and Quantitative analysis of land cover change in the deforested areas. It became evident that deforestation did not exhibit a standardized behavior, due to the large proportion of deforested areas in 2008 and the abrupt reduction in 2012. Furthermore, the deforestation in 2012, 2016, and 2020 resulted in greater conversion of native forests into pasture than the regeneration of these areas. Therefore, it can be concluded that 2008 had the highest deforestation rate, due to the increase in soybean prices, and that 2012 had the lowest rates due to the implementation of environmental policies, highlighting the relationship between environmental policies and the seasonality of commodity prices with deforestation rates. It was also concluded that the main destination for the deforested areas has been pastureland for livestock, and a viable solution to identify and punish offenders, as well as support small-scale producers and recover areas devastated by deforestation, is land regularization.