bluuebunny/crossref_metadata_2025 huggingface dataset:
MUTU KIMIA DAN ORGANOLEPTIK NUGGET IKAN BARAKUDA (Sphyraena jello), DENGAN SUBTITUSI TEPUNG TAPIOKA DAN TEPUNG WORTEL (Daucus carota L.)
Authors: Sali Fitriani , Asnani Asnani , Suwarjoyowirayatno Suwarjoyowirayatno
Published: October 2021

ABSTRACT         This study aims to determine the chemical quality of horse coarse nuggets, with tapioca and carrot flour substitution (Discuss carota L). This study used a complete randomized design (CRD) consisting of four treatments N1 = 50% barracuda fish meat : tapioca flour 50% : carrot flour 0%.,  N2 = 50% barracuda fish meat : tapioca flour 45% : carrot flour 5%., N3 = 50% barracuda fish meat : tapioca flour 40% : carrot flour 10%., N4 = 50% barracuda fish meat : tapioca flour 35% : carrot flour 15% flour each treatment was repeated three times. Test results were analyzed using analysis of variance (ANOVA) analysis of variance if there were real differences, a multiple range test (DMRT) was performed with a confidence level of 95%. The results showed that the substitution of tapioca and carrot flour had a very significant effect on the highest value of color 8.1, aroma 7.4, texture 7.1. Chemical test of fish nugget with tapioca and carrot flour substitution are water content 35.74%, ash content 4.63%, protein content 15.83%, fat content 11.37%, crude fiber 6.26% and antioxidant 242.15 g/mL. Keywords: Barracuda fish, carrot flour, chemical test, nuggets, organoleptic test, tapioca flour.ABSTRAKPenelitian ini bertujuan untuk mengetahui mutu kimia nugget ikan barakuda dengan subtitusi tepung  tapioka dan tepung wortel. Penelitian ini menggunakan rancangan acak lengkap RAL yang terdiri dari empat perlakuan N1 = daging ikan barakuda 50% : tepung tapioka 50% : tepung wortel 0%., N2 = daging ikan barakuda 50% : tepung tapioka 45% : tepung wortel 5%., N3 = daging ikan barakuda 50% : tepung tapioka 40% : tepung wortel 10%., N4 = daging ikan barakuda 50% : tepung tapioka 35% : tepung wortel 15% masing- masing perlakuan diulang sebanyak tiga kali ulangan. Data hasil pengujian dianalisis menggunakan analisis ragam (ANOVA) analisis of variens jika terdapat  perbedaan nyata dilakukan uji Duncan multiple rangge test (DMRT) dengan tingkat kepercayaan 95%. Hasil penelitian menunjukan bahwa subtitusi tepung tapioka dan tepung wortel (Daucus carota L) berpengaruh sangat nyata terhadap nilai tertinggi warna 8.1, aroma 7.4, tekstur 7.1, pada uji kimia nugget ikan dengan subtitusi tepung tapioka dan wortel kadar air 35.74%, kadar abu 4.63%, kadar protein 15.83%, kadar lemak 11.37%, serat kasar 6.26% dan antioksidan 242.15 g/mL.Kata kunci: Ikan barakuda, nugget, tepung tapioka, tepung wortel, uji kimia, uji organoleptik.
PENGARUH PENAMBAHAN Eucheuma cottonii DAN Sargassum sp. TERHADAP KOMPOSISI KIMIA, AKTIVITAS ANTIOKSIDAN SERTA SIFAT SENSORI SELAI UBI JALAR UNGU (Ipomoea batatas Poir)
Authors: Kalsum Umi , Asnani Asnani , Isamu Kobajashi Togo
Published: October 2021

The effect of addition of Eucheuma cotttonii and Sargassum sp. on chemical composition, antioxidant activity and sensory properties of purple sweet potato jam (Ipomoea batatas Poir) ABSTRACT         This study aims to determine the effect of the addition of Eucheuma cottonii and Sargassum sp. on chemical composition, antioxidant activity and sensory properties of purple sweet potato jam. This study used a Completely Randomized Design (CRD) consisting of four treatments using the combination of Sargassum sp., E. cottonii and purple sweet potatoes respectively: 45: 5: 50% (S1), 40: 10: 50% (S2), 35 : 15 : 50 % (S3), 30 : 20 : 50% (S4) and repeat three times. The results of the research obtained showed a real effect on sensory values which included color, aroma, and texture. The test results of the chemical content of jam showed water content between 27.62 %, ash content 1.59 %, crude fiber 1.44 % and carbohydrates 88.96 %. The test results of antioxidant content with percentage inhibitor (IC50) that is 337.81 μg / mL respectively. The results of this study indicate that there is an influence on the content of water content, ash, crude fiber, total carbohydrates and antioxidant content present in the purple sweet potato jam in each treatment.Keywords: Eucheuma cottonii, Ipomoea batatas, chemical composition and antioxidants, Sargassum sp., Jam, Sensory test ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh penambahan rumput laut jenis Eucheuma cottonii dan Sargassum sp. terhadap komposisi kimia, aktivitas antioksidan dan sifat sensori selai ubi jalar ungu. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari  empat perlakuan kombinasi penggunaan Sargassum sp., E. cottonii dan ubi jalar ungu, yaitu berturut-turut: 45: 5: 50 % (S1), 40: 10: 50 % (S2), 35: 15: 50 % (S3), serta 30: 20: 50 % (S4), ulangan sebanyak tiga kali. Hasil penelitian yang diperoleh menunjukkan pengaruh nyata terhadap nilai sensori yang meliputi warna, aroma dan tekstur. Hasil uji kandungan kimia selai menunjukkan kadar air 27,62 %, kadar abu 1,59%, serat kasar 1,44 % dan karbohidrat 88,96 %. Hasil uji kandungan antioksidan dengan nilai persentase penghambat (IC50) yaitu 337,81 g/mL. Hasil penelitian ini menunjukkan bahwa terdapat pengaruh terhadap kandungan kadar air, abu, serat kasar, karbohidrat total dan kandungan antioksidan yang ada pada selai ubi jalar ungu pada setiap perlakuan.Kata kunci: Eucheuma cottonii, Ipomoea batatas Poir, komposisi kimia dan antioksidan, Sargassum sp., Selai, uji sensori.
PENGARUH PROSES PEREBUSAN DAN PENGUKUSAN TERHADAP KANDUNGAN ALBUMIN DAN PROKSIMAT IKAN GABUS (Channa striata)
Authors: Salmatia Siti , Isamu Kobajashi Togo , Sartinah Ari
Published: October 2021

The Effect of The Boiling and Steaming Process on The Content of Albumin and Proximate Snakehead Fish (Channa striata). ABSTRACT         The aim of this study was to determine the effect of the boiling and steaming process on the content of albumin and proximate snakehead fish (Channa striata). This study used a complete randomized design (CRD) consisting of 3 treatments, namely: P1 = fresh, P2 = boiled and P3 = steamed and each treatment was repeated three times to obtain 9 experimental units. The data obtained were analyzed using analysis of variance (ANOVA), if the results of the analysis showed significant differences, then the Duncan's Multiple Range Test (DMRT) was performed to determine the real difference between treatments with a confidence level of 95% (a = 0.05). The results obtained showed that the effect of boiling and steaming processes on cork fish albumin levels were observed to have a significant effect where the value of albumin levels at P1 produced albumin levels was 0,35 mg/ml, P2 was 0,24 mg/ml and P3 was 0,27 mg/ml. Proximate analysis value on water content and protein content produced has a very significant effect, where the highest value of water content is in P2 of 76,25% and the highest value of protein content is in P2 of 21,52% and the proximate analysis of ash content and fat content snakehead fish has a significant effect where the highest value of ash content is in P3 of 1,2% and the highest value of fat content is in P1 of 1,79%. Keywords: albumin, snakehead fish, boiling, steaming.ABSTRAKTujuan penelitian ini adalah untuk mengetahui pengaruh proses perebusan dan pengukusan terhadap kandungan albumin dan proksimat ikan gabus (Channa striata). Penelitian ini menggunakan rancangan acak lengkap (RAL) yang terdiri dari 3 perlakuan, yaitu : P1 = segar, P2 = rebus dan P3 = kukus dan masing-masing perlakuan diulang sebanyak tiga kali sehingga diperoleh 9 unit percobaan. Data yang diperoleh dianalisis menggunakan analisis ragam (ANOVA), jika hasil analisis menunjukan beda nyata maka dilakukan uji Duncan’s Multiple Range Test (DMRT) untuk mengetahui beda nyata antar perlakuan dengan tingkat kepercayaan 95% (a=0,05). Hasil penelitian yang diperoleh menunjukkan bahwa pengaruh proses perebusan dan pengukusan terhadap kadar albumin ikan gabus yang diamati berpengaruh nyata dimana nilai kadar albumin pada P1 kadar albumin yang dihasilkan sebesar 0,35 mg/ml, P2 sebesar 0,24 mg/ml dan P3 sebesar 0,27 mg/ml. Nilai analisis proksimat pada kadar air dan kadar protein yang dihasilkan berpengaruh sangat nyata, dimana nilai tertinggi kadar air terdapat pada P2 sebesar 76,25% dan nilai tertinggi kadar protein terdapat pada P2 sebesar 21,52% dan pada analisis proksimat kadar abu dan kadar lemak ikan gabus berpengaruh nyata dimana nilai tertinggi kadar abu terdapat pada P3 sebesar 1,2% dan nilai tertinggi kadar lemak terdapat pada P1 sebesar 1,79%.Kata kunci: Albumin, Ikan gabus, perebusan, pengukusan.
STUDI KUALITAS KIMIA DAN SENSORIK IKAN PINDANG BANDENG (Chanos chanos Forskal) TANPA DURI YANG DISIMPAN DALAM KEMASAN VAKUM SUHU AMBIENT
Authors: Miftahulkhair Rahdi Fajar , Patadjai Andi Besse , Suwarjoyowirayatno Suwarjoyowirayatno
Published: October 2021

ABSTRACT         The aim of this study was to determine the effect on the freshness quality of thorns leaves milkfish storing at ambient temperature in vacuum packaging. This study used a non-factorial randomized design (CRD) which was storage time consisting of 4 treatments H0 (0 days), H2 (2 days), H4 (4 days), and H6 (6 days). Each treatment was carried out with 3 times replication. Based on the test results, it showed that the difference in different storage duration significantly affected on freshness value of fish (TVB and TMA) of thorns leave milkfish at ambient temperature, along with the time of storage of vacuum packaged fish, the value of TVB and TMA increased on the 6th day of storage (H6) had the highest TVB and TMA values of 24.40 mgN / 100g and 3.43 mgN / 100g, respectively. Keywords: milkfish leave thorns, pindang, sensory, vacuum.ABSTRAKTujuan dari penelitian ini adalah untuk mengetahui nilai kesegaran ikan pindang bandeng tanpa duri kemasan vakum pada suhu ambient. Penelitian ini menggunakan rancangan percobaan adalah Rancangan Acak Lengkap (RAL) non faktorial yakni waktu penyimpanan yang terdiri dari 4 perlakuan yaitu H0 ( 0 hari), H2 (2 hari), H4 (4 hari) dan H6 (6 hari). Masing-masing perlakuan dilakukan sebanyak tiga kali pengulangan, sehingga diperoleh jumlah satuan percobaan sebanyak 12 unit. Berdasarkan hasil pengujian, perbedaan lama penyimpanan berbeda secara signifikan mempengaruhi nilai kesegaran ikan (TVB dan TMA) bandeng tanpa duri yang disimpan pada suhu ambeien, seiring dengan  waktu penyimpanan ikan pindang bandeng tanpa duri kemasan vakum, nilai TVB dan TMA meningkat. Pada hari ke 6 penyimpanan (H6) memiliki nilai TVB dan TMA tertinggi masing-masing 24,40 mgN/100g dan 3,43 mgN/100g.Kata kunci: ikan bandeng tanpa duri, pindang, vakum
UJI STABILITAS PENYIMPANAN IKAN PEPEREK (Leiognathus equulus) KERING DENGAN METODE PENGEMASAN YANG BERBEDA
Authors: Rusnawati Rusnawati , Haslianti Haslianti , Suwarjoyowirayatno Suwarjoyowirayatno
Published: October 2021

ABSTRACT         The aim of this study was to determine the storage stability test of dried peperek fish (Leiognathus equulus) with different packaging methods. This study used a randomized block design (RBD) consisting of 2 treatment factors. The first treatment factor is PE plastic packaging (VO) and aluminum foil plastic packaging (V1). The second treatment factor is storage time which consists of 4 levels, namely H1 (1 day), H2 (7 days), H3 (14 days), and H4 (21 days). Test parameters include organoleptic tests (appearance, aroma, and texture), TPC, and moisture content. Based on the results, it showed that the effect of storage time of PE plastic packaging and aluminum foil plastic packaging on organoleptic tests is appearance V0T14 8.32, V1H1 6.64, aroma V0T14 8.36, V1H1  3.69, texture V0T14 8.52,  V1H1 4. while the best moisture content test results are found in V1H14 with the lowest value of 13.94% and lowest TPC content in V1H14 with the lowest value of 1.46x105. Keywords: fish peperek (leiognathuus equlus), Total Plate Count TPC, water content test, and organolepticABSTRAKPenelitian ini bertujuan untuk mengetahui uji stabilitas penyimpanan ikan peperek (Leiognathus equulus) kering dengan metode pengemasan yang berbeda. Penelitian ini menggunakan rancangan acak kelompok (RAK) yang terdiri dari 2 faktor perlakuan. Faktor perlakuan pertama yaitu kemasan plastik pe (VO) dan kemasan plastik aluminium foil (V1). Faktor perlakuan kedua adalah waktu penyimpanan yang terdiri dari 4 taraf yaitu H1 (1 hari), H2 (7 hari), H3 (14 hari) dan H4 (21 hari). Parameter uji meliputi Uji organoleptik (kenampakan, aroma, dan tekstur), TPC dan kadar air. Berdasarkan hasil penelitian, menunjukkan bahwa pengaruh lama penyimpanan kemasan plastik PE dan kemasan plastik aluminium foil terhadap uji organoleptik yaitu kenampakan V0T14 8.32, V1H1 6.64, aroma V0T14 8.36, V1H1  3.69 tekstur V0T14 8.52,  V1H1 4. sedangkan hasil uji kadar air  terbaik terdapat pada V1H14 dengan nilai terendah 13.94%, dan kandungan TPC terendah V1H14 dengan nilai terendah 1.46x105.Kata kunci: Ikan peperek (leiognathuus equlus), Total Plate Count TPC, uji kadar air, dan uji organoleptik
UJI KANDUNGAN KIMIA DAN CEMARAN LOGAM KERANG KIMA (Tridacna sp.) KERING YANG DIPERDAGANGKAN DI KABUPATEN KONAWE UTARA SULAWESI TENGGARA
Authors: Alam Samsul , Isamu Kobajashi Togo , Suwarjoyowirayatno Suwarjoyowirayatno
Published: October 2021

The Test of Chemical Content and Metal Contamination of Dry Clam (Tridacna sp.) Traded in North Konawe Regency Southeast SulawesiABSTRACT         The aim of this study was to determine the characteristics of chemical composition (water, ash, protein, fat, and carbohydrate) and Metal Contamination of Dry Clam. The analysis of the data used in this study is to use descriptive analysis. The results of the analysis of the chemical content of the highest treatment of water content are found in P2 with a value of 25.84%. The highest ash content was found in the P2 treatment with a value of 13.84%. The highest level of protein treatment was at P3 with a value of 14.28%. The highest treatment fat content was in P3 with a value of 2.43% and the highest treatment carbohydrate content was in P2 with a value of 1.88% and. The results of the analysis of heavy metals were the highest Cd levels were at P3 with a value of 0.074 mg / Kg and the lowest was at P1 0.057 mg / Kg and the highest Pb metal at P3 0.026 mg / Kg the lowest was at P1 0.016 mg / Kg.Keywords: Clam (Tridacna sp.), Chemical content, Heavy metals ABSTRAKTujuan penelitian ini adalah untuk mengetahui karakteristik komposisi kimiawi (air, abu, protein, lemak, dan karbohidrat) dan cemaran Logam berat pada Kerang kima Kering. Analisis data yang digunakan dalam penelitian ini adalah menggunakan analisis deskriptif. Hasil analisis kadar kimia perlakuan kadar air tertinggi terdapat pada P2 dengan nilai 25,84%. Kadar abu tertinggi terdapat pada perlakuan P2 dengan nilai 13,84%. Perlakuan protein tertinggi terdapat pada P3 dengan nilai 14,28%. Kadar lemak perlakuan tertinggi terdapat pada P3 dengan nilai 2,43% dan kadar karbohidrat perlakuan tertinggi terdapat pada P2 dengan nilai 1,88% dan. Hasil analisis logam berat kadar Cd tertinggi pada P3 dengan nilai 0,074 mg / Kg dan terendah pada P1 0,057 mg / Kg dan logam Pb tertinggi pada P3 0,026 mg / Kg, terendah pada P1. 0,016 mg / Kg.Kata kunci Kerang kima (Tridacna sp.) kering, Kandungan Kimia, Logam berat
KARAKTERISTIK MUTU SIRUP RUMPUT LAUT (Eucheuma spinosum)
Authors: Anharullah La Ode Muhammad , Haslianti Haslianti , Asyik Nur
Published: October 2021

ABSTRACT         The aim of this study was to determine the quality characteristics of seaweed Syrup (Eucheuma spinosum). This study used a Completely Randomized Design (CRD) consisting of 3 treatments, namely: P1 = 100 gr seaweed: 200 gr sugar: 3.5 L water: CMC 0.3 gr, P2 = 120 gr seaweed: 200 granulated sugar gr: water 3,5 L: CMC 0,3 gr, P3 = Seaweed 140 gr: sugar 200 gr: water 3,5 L: CMC 0,3 gr: with three times replications. Data from observations were analyzed using ANOVA (Analysis of Variance) at a level of 95%, if there is a significant difference (P> 0.05), further tests were carried out with the DMRT (Duncan Multiple Range Test) tests at a 95% confidence level. The results obtained showed that the concentration of seaweed (E. spinosum), water, and taste did not significantly affect the aroma and taste of seaweed syrup. The best treatment was in the treatment of P1 with a scent value of 3.46 with a rather like category, and P1 for a taste of 3.46 with a somewhat like category. The chemical value of seaweed syrup is the value of water content ranging from 38.96% -43.21%, ash content ranges from 0.82-1.34%. Keywords: Syrup, seaweed (Eucheuma spinosum), chemistry and sensoryABSTRAKTujuan penelitian ini adalah untuk mengetahui Karakteristik Mutu Sirup Rumput Laut (Eucheuma spinosum). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 3 perlakuan yaitu : P1 = Rumput laut 100 gr : gula pasir 200 gr : air 3,5 L : CMC 0,3 gr, P2 = Rumput laut 120 gr : gula pasir 200 gr: air 3,5 L : CMC 0,3 gr, P3 = Rumput laut 140 gr : gula pasir 200 gr : air 3,5 L : CMC 0,3 gr : dan ulangan sebanyak tiga kali. Data hasil pengamatan dianalisa menggunakan ANOVA (Analysis of Variance) pada taraf 95%, apabila terdapat beda nyata (P>0,05) maka dilakukan uji lanjut dengan uji DMRT (Duncan Multiple Range Test) pada taraf kepercayaan 95%. Hasil penelitian yang diperoleh menunjukkan bahwa Kosentrasi rumput laut (E. Spinosum), air dan rasa berpengaruh tidak nyata terhadap aroma dan rasa sirup rumput laut. Perlakuan terbaik terdapat pada perlakuan P1 dengan nilai aroma sebesar 3,46 dengan kategori  agak suka, dan P1 rasa 3,46 dengan kategori agak suka. Nilai kimia sirup rumput laut yaitu nilai kadar air berkisar 38,96%-43,21%, kadar abu berkisar 0,82-1,34%.Kata kunci: Sirup, rumput laut (Eucheuma spinosum), kimia dan sensori
ANALISIS PENGGUNAAN RODHAMIN B PADA PRODUK TERASI YANG ADA DISEKITAR MASYARAKAT KECAMATAN POLEANG TENGAH KABUPATEN BOMBANA, SULAWESI TENGGARA
Authors: Aminarti Aminarti , Ibrahim Mohamad Nuh , Sadimantara Muhammad Syukri
Published: October 2021

ABSTRACT         This research was conducted by Descriptive method to obtain the required information. The way of data collection is by taking samples of shrimp paste in Kampung Baru Village (A), Lampopala Village (B), and Lauru Village (C) and then doing rodhamin B dye testing on the three shrimp paste samples. The results of Analysis of Rodhamin B in shrimp paste Products around the Community of Central Poleang District Bombana regency, Southeast Sulawesi in Kampung Baru Village (A), Lampopala Village (B), and Lauru Village (C) received negative results. Keywords: qualitative analysis, rhodamin B, shrimp paste.ABSTRAKPenelitian ini dilakukan dengan metode Deskriptif untuk memperoleh informasi yang dibutuhkan. Cara pengumpulan data yaitu dengan mengambil sampel terasi yang ada di Desa Kampung Baru (A), Desa Lampopala (B), dan Desa Lauru (C) lalu melakukan pengujian pewarna Rodhamin B pada ketiga sampel terasi tersebut. Hasil Analisis Penggunaan Rodhamin B pada Produk Terasi yang ada di sekitar Masyarakat Kecamatan Poleang Tengah Kabupaten Bombana, Sulawesi Tenggara pada Desa Kampung Baru (A), Desa Lampopala (B), dan Desa Lauru (C) mendapatkan hasil negatif.Kata kunci: analisis kualitatif, rhodamin B, terasi.
KARAKTERISASI IKAN TERI ASIN (Stolephorus sp.) YANG DIPRODUKSI DI DESA WABURENSE, KECAMATAN MAWASANGKA, KABUPATEN BUTON TENGAH
Authors: Taufik Taufik , Isamu Kobajashi Togo , Suwarjoyowirayatno Suwarjoyowirayatno
Published: October 2021

ABSTRACT         The aims of this study were to determine the proximate content (water content, ash, fat, protein), and salt content. Data analysis was used descriptive analysis to provide a general description of the data. Proximate Test Results on Salted anchovy water content ranged from 18.61% -19.68%, salted anchovy ash content from 12.69% -14.26%, protein content 37.37% -41.25% , fat content from 1.05% -1.18% and salt content from 12.62% -13.42%. Keywords: Salted anchovy, proximate, and salinityABSTRAKPeneletian ini bertujuan untuk mengetahui kandungan proksimat (kadar air, kadar abu, kadar lemak, kadar protein), dan kadar garam. analisis data penelitian ini menggunakan analisis deskriptif untuk memberikan gambaran umum tentang data. Hasil uji proksimat pada kadar air ikan teri asin berkisar 18,61%-19,68% ,kadar abu ikan teri asin berkisar 12,69%-14,26%, kadar protein ikan teri asin 37,37%-41,25%, kadar lemak ikan teri asin berkisar 1,05%-1,18% dan kadar garam berkisar antara 12,62%-13,42%.Kata kunci: Ikan teri asin, proksimat, kadar garam
UJI FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN DARI RUMPUT LAUT Sargassum sp. DENGAN METODE PENGERINGAN YANG BERBEDA
Authors: Maulana Fazirul , Asnani Asnani , Haslianti Haslianti
Published: October 2021

ABSTRACT         Sargassum sp. is the most abundant type of seaweed from the brown algae group (Phaeophyceae) which is spread in tropical waters. The aim of this study was to determine the phytochemical content and antioxidant activity of the extract of Sargassum sp. with different drying methods. Sargassum sp. dried using room temperature (± 25ºC) for 21 days and oven (40oC) for 30 hours, then extracted using methanol. Phytochemical tests were carried out to determine the presence of alkaloids, tannins, flavonoids, saponins, steroids, and triterpenoids. The antioxidant activity of the seaweed extract of Sargassum sp. tested by the DPPH method (1-1-diphenyl-2-picrylhydrazyl). Phytochemical test results of the extract Sargassum sp. flavonoids, saponins, triterpenoids, and steroids, while alkaloids and tannins were not found. The value of antioxidant activity in the methanol extract of Sargassum sp. shows a higher room temperature treatment with an IC50 value of 823.652 ppm compared to a treatment temperature of 40oC with an IC50 value of 914.1920 ppm. The drying method affects the antioxidant activity of Sargassum sp. Keywords: Sargassum sp., drying, extraction, phytochemicals, antioxidants.ABSTRAKSargassum sp. merupakan jenis rumput laut paling melimpah dari kelompok alga coklat (Phaeophyceae) yang tersebar di perairan tropis. Tujuan penelitian adalah untuk mengetahui kandungan fitokimia dan aktivitas antioksidan ekstrak Sargassum sp. dengan metode pengeringan yang berbeda. Sargassum sp. dikeringkan menggunakan suhu ruang (± 25ºC) selama 21 hari dan oven (suhu 40oC) selama 30 jam, kemudian diekstrak menggunakan pelarut metanol. Uji fitokimia dilakukan untuk mengetahui keberadaan senyawa alkaloid, tanin, flavonoid, saponin, steroid dan triterpenoid pada ekstrak. Aktivitas antioksidan ekstrak rumput laut Sargassum sp. diuji dengan metode DPPH (1-1-difenil-2-pikrilhidrazil). Hasil uji fitokimia pada ekstrak Sargassum sp. yang dikeringkan pada suhu ruang dan suhu 40oC ditemukan adanya flavonoid, saponin, triterpenoid dan steroid sedangkan alkaloid dan tannin tidak ditemukan. Nilai aktivitas antioksidan pada ekstrak metanol Sargassum sp. menunjukan perlakuan suhu ruang lebih tinggi dengan nilai IC50 823,652 ppm dibandingkan perlakuan suhu 40oC dengan nilai IC50 914,1920 ppm. Metode pengeringan berpengaruah terhadap aktivitas antioksidan dari Sargassum sp.Kata kunci: Sargassum sp., pengeringan, ekstraksi, fitokimia, antioksidan.
CÁLCULO DIFERENCIAL E INTEGRAL: RELATO DE UMA EXPERIÊNCIA ENVOLVENDO PRÁTICA AUDIOVISUAL E ESTILOS DE APRENDIZAGEM
Authors: Souza Taciana Oliveira , Santos Vanessa Matos dos
Published: October 2021

O processo de ensino-aprendizagem da matemática tem se traduzido como um dos maiores desafios para educadores brasileiros de todos os níveis da educação escolarizada. Lacunas de aprendizagem se tornam mais evidentes no ensino superior, que exige do aluno cada vez mais poder de síntese e abstração frente aos problemas cotidianos. Partindo deste contexto, o presente texto é um relato de uma experiência realizada no âmbito da disciplina "Cálculo Diferencial e Integral" para cursos de graduação selecionados em uma universidade federal, entre 2017 e 2018. Resultante de projeto interdisciplinar entre as áreas de Matemática e Comunicação, a experiência contou com a produção de audiovisuais e também com a adaptação de estratégias didáticas com o respaldo teórico-metodológico dos Estilos de Aprendizagem. Os resultados obtidos demonstram que as ações desenvolvidas apresentaram importantes contribuições no âmbito da educação matemática.
O PNAIC DE ALFABETIZAÇÃO MATEMÁTICA NO MUNICÍPIO DE CURITIBA: UM OLHAR A PARTIR DO CICLO DE POLÍTICAS PÚBLICAS EDUCACIONAIS
Authors: Nunes Vitorino Viviane da Cruz Leal , Rolkouski Emerson
Published: October 2021

O objetivo desse artigo é compreender os impactos da formação do PNAIC – Pacto Nacional pela Alfabetização na Idade Certa - de Alfabetização Matemática na trajetória profissional e na prática pedagógica das professoras participantes no município de Curitiba. Para tanto, utilizamos os pressupostos do ciclo de políticas públicas educacionais, composto por três contextos principais: influência, produção de texto e prática. Para o contexto de influência, fizemos um estudo sociopolítico da época da criação e implantação do PNAIC, destacando o papel da sociedade civil organizada para que o tema da alfabetização fosse privilegiado na agenda política. Para o contexto da produção de texto, enfatizamos as leis e os Cadernos de Formação do PNAIC de Alfabetização Matemática. No contexto da prática, utilizamos as narrativas de professoras que participaram do referido programa do PNAIC, realizadas de acordo com os aportes metodológicos da História Oral. Por meio dessas narrativas, após análise, verificamos que o PNAIC causou impacto na reflexão sobre a alfabetização matemática, na prática cotidiana e na vida profissional das entrevistadas. A pesquisa ainda reforça as narrativas como fontes históricas, potenciais objetos de pesquisa para outros pesquisadores, lançando novos olhares e perspectivas. Espera-se que os resultados aqui publicizados contribuam para a elaboração e reelaboração de políticas públicas educacionais e para aqueles que se dedicam à compreensão de suas potencialidades e limitações.
Exosomal miR-500 Derived From Lipopolysaccharide-Treated Macrophage Accelerates Liver Fibrosis by Suppressing MFN2
Authors: Chen Lisha , Huang Yan , Duan Zhixi , Huang Peiqi , Yao Hongbing , Zhou Yu , Ji Qin , Liu Xiangfeng
Published: October 2021

Liver fibrosis is an outcome of chronic hepatic injury, which can eventually result in cirrhosis, liver failure, and even liver cancer. The activation of hepatic stellate cell (HSC) is a prominent driver of liver fibrosis. Recently, it has been found that the crosstalk between HSCs and immune cells, including hepatic macrophages, plays an important role in the initiation and development of liver fibrosis. As a vital vehicle of intercellular communication, exosomes transfer specific cargos into HSCs from macrophages. Here, we show that exosomes derived from lipopolysaccharide (LPS)-treated macrophages has higher expression level of miR-500. And overexpression or inhibition of miR-500 in macrophage exosomes could promote or suppress HSC proliferation and activation. Treatment of exosomes with miR-500 overexpression can accelerate liver fibrosis in CCl4-induced liver fibrosis mouse model. miR-500 promotes HSC activation and liver fibrosis via suppressing MFN2. Moreover, miR-500 in serum exosomes could be a biomarker for liver fibrosis. Taken together, exosomal miR-500 derived from LPS-activated macrophages promotes HSC proliferation and activation by targeting MFN2 in liver fibrosis.
Mitochondrial Mutations in Ethambutol-Induced Optic Neuropathy
Authors: Zhang Xiao-Hui , Xie Yue , Xu Quan-Gang , Cao Kai , Xu Ke , Jin Zi-Bing , Li Yang , Wei Shi-Hui
Published: October 2021

Background: Ethambutol-induced optic neuropathy (EON) is a well-recognized ocular complication in patients who take ethambutol as a tuberculosis treatment. The aim of the current study was to investigate the presence of mitochondrial mutations, including OPA1 and Leber’s hereditary optic neuropathy (LHON)-mitochondrial DNA (mtDNA), in patients with EON and to determine their effect on clinical features of these patients.

Methods: All 47 patients underwent clinical evaluations, including best-corrected visual acuity, fundus examination, and color fundus photography; 37 patients were then followed up over time. Molecular screening methods, including PCR-based sequencing of the OPA1 gene and LHON-mtDNA mutations, together with targeted exome sequencing, were used to detect mutations.

Results: We detected 15 OPA1 mutations in 18 patients and two LHON-mtDNA mutations in four patients, for an overall mutation detection rate of 46.8%. The mean presentation age was significantly younger in the patients with the mitochondrial mutations (27.5 years) than in those without mutations (48 years). Fundus examination revealed a greater prevalence of optic disc hyperemia in the patients with mutations (70.5%) than without mutations (48%). Half of the patients with mutations and 91% of the patients without mutations had improved vision. After adjusting for confounders, the logistic regression revealed that the patients with optic disc pallor on the first visit (p = 0.004) or the patients with the mitochondrial mutations (p < 0.001) had a poorer vision prognosis.

Conclusion: Our results indicated that carriers with OPA1 mutations might be more vulnerable for the toxicity of EMB to develop EON.
Schistosome-Associated Pulmonary Arterial Hypertension: A Review Emphasizing Pathogenesis
Authors: Ferrari Teresa Cristina Abreu , Albricker Ana Cristina Lopes , Gonçalves Ina Morais , Freire Cláudia Maria Vilas
Published: October 2021

Schistosomiasis, especially due to Schistosoma mansoni, is a well-recognized cause of pulmonary arterial hypertension (PAH). The high prevalence of this helminthiasis makes schistosome-related PAH (Sch-PAH) one of the most common causes of this disorder worldwide. The pathogenic mechanisms underlying Sch-PAH remain largely unknown. Available evidence suggests that schistosome eggs reach the lung via portocaval shunts formed as a consequence of portal hypertension due to hepatosplenic schistosomiasis. Once deposited into the lungs, the eggs elicit an immune response resulting in periovular granuloma formation. Immune mediators drive transforming growth factor-β (TGF-β) release, which gives rise to pulmonary vascular inflammation with subsequent remodeling and development of angiomatoid and plexiform lesions. These mechanisms elicited by the eggs seem to become autonomous and the vascular lesions progress independently of the antigen. Portopulmonary hypertension, which pathogenesis is still uncertain, may also play a role in the genesis of Sch-PAH. Recently, there have been substantial advances in the diagnosis and treatment of PAH, but it remains a difficult condition to recognize and manage, and patients still die prematurely from right-heart failure. Echocardiography is used for screening, and the formal diagnosis requires right-heart catheterization. The experience in treating Sch-PAH is largely limited to the phosphodiesterase type 5 inhibitors, with evidence suggesting that these vasodilators improve symptoms and may also improve survival. Considering the great deal of uncertainty about Sch-PAH pathogenesis, course, and treatment, the aim of this review is to summarize current knowledge on this condition emphasizing its pathogenesis.
Monte Carlo Ray-Tracing Simulation of a Cassegrain Solar Concentrator Module for CPV
Authors: Oh Seung Jin , Kim Hyungchan , Hong Youngsun
Published: October 2021

The concentration ratio is one of the most important characteristics in designing a Cassegrain solar concentrator since it directly affects the performance of high-density solar energy applications such as concentrated photovoltaics (CPVs). In this study, solar concentrator modules that have different configurations were proposed and their performances were compared by means of a Monte Carlo ray-tracing algorithm to identify the optimal configurations. The first solar concentrator design includes a primary parabolic concentrator, a parabolic secondary reflector, and a homogenizer. The second design, on the other hand, includes a parabolic primary concentrator, a secondary hyperbolic concentrator, and a homogenizer. Two different reflectance were applied to find the ideal concentration ratio and the actual concentration ratio. In addition, uniform rays and solar rays also were compared to estimate their efficiency. Results revealed that both modules show identical concentration ratios of 610 when the tracking error is not considered. However, the concentration ratio of the first design rapidly drops when the sun tracking error overshoots even 0.1°, whereas the concentration ratio of the second design remained constant within the range of the 0.8° tracking error. It was concluded that a paraboloidal reflector is not appropriate for the second mirror in a Cassegrain concentrator due to its low acceptance angle. The maximum collection efficiency was achieved when the f-number is smaller and the rim angle is bigger and when the secondary reflector is in a hyperboloid shape. The target area has to be rather bigger with a shorter focal length for the secondary reflector to obtain a wider acceptance angle.
Memories of Migrations Past: Sociality and Cognition in Dynamic, Seasonal Environments
Authors: Gurarie Eliezer , Potluri Sriya , Cosner George Christopher , Cantrell Robert Stephen , Fagan William F.
Published: October 2021

Seasonal migrations are a widespread and broadly successful strategy for animals to exploit periodic and localized resources over large spatial scales. It remains an open and largely case-specific question whether long-distance migrations are resilient to environmental disruptions. High levels of mobility suggest an ability to shift ranges that can confer resilience. On the other hand, a conservative, hard-wired commitment to a risky behavior can be costly if conditions change. Mechanisms that contribute to migration include identification and responsiveness to resources, sociality, and cognitive processes such as spatial memory and learning. Our goal was to explore the extent to which these factors interact not only to maintain a migratory behavior but also to provide resilience against environmental changes. We develop a diffusion-advection model of animal movement in which an endogenous migratory behavior is modified by recent experiences via a memory process, and animals have a social swarming-like behavior over a range of spatial scales. We found that this relatively simple framework was able to adapt to a stable, seasonal resource dynamic under a broad range of parameter values. Furthermore, the model was able to acquire an adaptive migration behavior with time. However, the resilience of the process depended on all the parameters under consideration, with many complex trade-offs. For example, the spatial scale of sociality needed to be large enough to capture changes in the resource, but not so large that the acquired collective information was overly diluted. A long-term reference memory was important for hedging against a highly stochastic process, but a higher weighting of more recent memory was needed for adapting to directional changes in resource phenology. Our model provides a general and versatile framework for exploring the interaction of memory, movement, social and resource dynamics, even as environmental conditions globally are undergoing rapid change.
Targeting DCs for Tolerance Induction: Don’t Lose Sight of the Neutrophils
Authors: Hafkamp Florianne M. J. , Groot Kormelink Tom , de Jong Esther C.
Published: October 2021

Chronic inflammatory disorders (CID), such as autoimmune diseases, are characterized by overactivation of the immune system and loss of immune tolerance. T helper 17 (Th17) cells are strongly associated with the pathogenesis of multiple CID, including psoriasis, rheumatoid arthritis, and inflammatory bowel disease. In line with the increasingly recognized contribution of innate immune cells to the modulation of dendritic cell (DC) function and DC-driven adaptive immune responses, we recently showed that neutrophils are required for DC-driven Th17 cell differentiation from human naive T cells. Consequently, recruitment of neutrophils to inflamed tissues and lymph nodes likely creates a highly inflammatory loop through the induction of Th17 cells that should be intercepted to attenuate disease progression. Tolerogenic therapyviaDCs, the central orchestrators of the adaptive immune response, is a promising strategy for the treatment of CID. Tolerogenic DCs could restore immune tolerance by driving the development of regulatory T cells (Tregs) in the periphery. In this review, we discuss the effects of the tolerogenic adjuvants vitamin D3 (VD3), corticosteroids (CS), and retinoic acid (RA) on both DCs and neutrophils and their potential interplay. We briefly summarize how neutrophils shape DC-driven T-cell development in general. We propose that, for optimization of tolerogenic DC therapy for the treatment of CID, both DCs for tolerance induction and the neutrophil inflammatory loop should be targeted while preserving the potential Treg-enhancing effects of neutrophils.
The Discovery and Preliminary Geological and Faunal Descriptions of Three New Steinahóll Vent Sites, Reykjanes Ridge, Iceland
Authors: Taylor James , Devey Colin , Le Saout Morgane , Petersen Sven , Kwasnitschka Tom , Frutos Inmaculada , Linse Katrin , Lörz Anne-Nina , Pałgan Dominik , Tandberg Anne H. , Svavarsson Jörundur , Thorhallsson Daniel , Tomkowicz Adrianna , Egilsdóttir Hrönn , Ragnarsson Stefán Á. , Renz Jasmin , Markhaseva Elena L. , Gollner Sabine , Paulus Eva , Kongsrud Jon , Beermann Jan , Kocot Kevin M. , Meißner Karin , Bartholomä Alexander , Hoffman Leon , Vannier Pauline , Marteinsson Viggó Þ. , Rapp Hans T. , Díaz-Agras Guillermo , Tato Ramiro , Brix Saskia
Published: October 2021

During RVMS Merianexpedition MSM75, an international, multidisciplinary team explored the Reykjanes Ridge from June to August 2018. The first area of study, Steinahóll (150–350 m depth), was chosen based on previous seismic data indicating hydrothermal activity. The sampling strategy included ship- and AUV-mounted multibeam surveys, Remotely Operated Vehicle (ROV), Epibenthic Sledge (EBS), and van Veen grab (vV) deployments. Upon returning to Steinahóll during the final days of MSM75, hydrothermal vent sites were discovered using the ROV Phoca (Kiel, GEOMAR). Here we describe and name three new, distinct hydrothermal vent site vulnerable marine ecosystems (VMEs); Hafgufa, Stökkull, Lyngbakr. The hydrothermal vent sites consisted of multiple anhydrite chimneys with large quantities of bacterial mats visible. The largest of the three sites (Hafgufa) was mapped, and reconstructed in 3D. In total 23,310 individual biological specimens were sampled comprising 41 higher taxa. Unique fauna located in the hydrothermally venting areas included two putative new species of harpacticoid copepod (Tisbesp. nov. andAmphiascussp. nov.), as well as the spongeLycopodina cupressiformis(Carter, 1874). Capitellidae Grube, 1862 and Dorvilleidae Chamberlin, 1919 families dominated hydrothermally influenced samples for polychaetes. Around the hydrothermally influenced sites we observed a notable lack of megafauna, with only a few species being present. While we observed hydrothermal associations, the overall species composition is very similar to that seen at other shallow water vent sites in the north of Iceland, such as the Mohns Ridge vent fields, particularly with peracarid crustaceans. We therefore conclude the community overall reflects the usual “background” fauna of Iceland rather than consisting of “vent endemic” communities as is observed in deeper vent systems, with a few opportunistic species capable of utilizing this specialist environment.
Differences in Carbon Acquisition Could Explain Adaptive Responses in a Baltic Sea Pico-Phytoplankton
Authors: Listmann Luisa , Kerl Franziska , Martens Nele , Schaum C-Elisa
Published: October 2021

Pico-phytoplankton have ample scope to react to environmental change. Nevertheless, we know little about the underlying physiological mechanisms that govern how evolutionary history may affect short-term responses to environmental change. We investigated growth rates and carbon uptake related traits at 15° and 22°C and at different times during the microbial growth curve (lag phase, mid and late exponential) of eight novel strains of Ostreococcus sp. (ca. 1 μm). The strains were isolated from two distinct regions of the Baltic Sea differing in salinity and temperature as well as variability therein from North-East (Bornholm Basin) to South-West (Kiel area). Strains from Kiel area had ca. 10% higher growth rates on average and showed more variation between strains compared to strains from the Bornholm Basin. While biomass increased throughout the experiment in both temperature, CUE (carbon use efficiency, indicative of photosynthetically derived carbon available for growth) was too low to explain positive growth throughout the entire growth curve at 15°C and during the early stages at 22°C. Throughout the growth curve CUE then increased enough to sustain growth, but only at 22°C. Consequently, we then tested whether Ostreococcus use organic carbon to supplement growth when light is not a limiting factor. We show that Ostreococcus qualitatively modulate their potential to grow on organic carbon sources throughout a single growth curve. Based on the differences between CUE and a potential to grow on organic carbon, we postulate a shift in carbon acquisition between inorganic and organic sources in Ostreococcus sp. with potential implications on ecological dynamics within microbial communities.
Numerical Analysis of the Effect of Binary Typhoons on Ocean Surface Waves in Waters Surrounding Taiwan
Authors: Chang Tzu-Yin , Chen Hongey , Hsiao Shih-Chun , Wu Han-Lun , Chen Wei-Bo
Published: October 2021

The ocean surface waves during Super Typhoons Maria (2018), Lekima (2019), and Meranti (2016) were reproduced using hybrid typhoon winds and a fully coupled wave-tide-circulation modeling system (SCHISM-WWM-III). The hindcasted significant wave heights are in good agreement with the along-track significant wave heights measured by the altimeters aboard the SARAL (Satellite with ARgos and ALtiKa) and Jason-2 satellites. Two numerical experiments pairing Super Typhoons Maria (2018) and Meranti (2016) and Super Typhoons Lekima (2019) and Meranti (2016) were conducted to analyze the storm wave characteristics of binary and individual typhoons. Four points located near the tracks of the three super typhoons were selected to elucidate the effects of binary typhoons on ocean surface waves. The comparisons indicate that binary typhoons not only cause an increase in the significant wave height simulations at four selected pints but also result in increases in the one-dimensional wave energy and two-dimensional directional wave spectra. Our results also reveal that the effects of binary typhoons on ocean surface waves are more significant at the periphery of the typhoon than near the center of the typhoon. The interactions between waves generated by Super Typhoons Maria (2018) and Meranti (2016) or Super Typhoons Lekima (2019) and Meranti (2016) might be diminished by Taiwan Island even if the separation distance between two typhoons is <700 km.
Disorganization of Oscillatory Activity in Animal Models of Schizophrenia
Authors: Speers Lucinda J. , Bilkey David K.
Published: October 2021

Schizophrenia is a chronic, debilitating disorder with diverse symptomatology, including disorganized cognition and behavior. Despite considerable research effort, we have only a limited understanding of the underlying brain dysfunction. In this article, we review the potential role of oscillatory circuits in the disorder with a particular focus on the hippocampus, a region that encodes sequential information across time and space, as well as the frontal cortex. Several mechanistic explanations of schizophrenia propose that a loss of oscillatory synchrony between and within these brain regions may underlie some of the symptoms of the disorder. We describe how these oscillations are affected in several animal models of schizophrenia, including models of genetic risk, maternal immune activation (MIA) models, and models of NMDA receptor hypofunction. We then critically discuss the evidence for disorganized oscillatory activity in these models, with a focus on gamma, sharp wave ripple, and theta activity, including the role of cross-frequency coupling as a synchronizing mechanism. Finally, we focus on phase precession, which is an oscillatory phenomenon whereby individual hippocampal place cells systematically advance their firing phase against the background theta oscillation. Phase precession is important because it allows sequential experience to be compressed into a single 120 ms theta cycle (known as a ‘theta sequence’). This time window is appropriate for the induction of synaptic plasticity. We describe how disruption of phase precession could disorganize sequential processing, and thereby disrupt the ordered storage of information. A similar dysfunction in schizophrenia may contribute to cognitive symptoms, including deficits in episodic memory, working memory, and future planning.
Systemic Inflammation Index Values Are Associated With Worsened Disease Severity and Poor Response to Autoimmune Encephalitis Treatment
Authors: Mei Yanliang , Yang Jing , Yuan Yanpeng , Liu Yutao , Liu Xiaojing , Li Mingli , Fan Shiheng , Li Lanjun , Jiang Chenyang , Xu Yuming
Published: October 2021

Both specific and innate immune responses play important roles in autoimmune encephalitis (AE). We aimed to explore the predictive value of the systemic inflammation index (SII) at admission as a peripheral biomarker of treatment response of AE. A total of 146 patients diagnosed with AE in the First Affiliated Hospital of Zhengzhou University from January 1, 2018 to September 22, 2020 were retrospectively and consecutively analyzed as per the inclusion criteria and divided into two groups according to their response to immunotherapy after 30 days. The predictive value of the SII as a peripheral biomarker for AE treatment response was calculated using the receiver operating characteristic curve analysis, which showed that the best SII cut-off value for predicting poor response to AE treatment was 863.3; the area under the curve was 0.75, with 83.0% sensitivity and 72.0% specificity. The risk factors for poor response to AE treatment were analyzed; univariable analysis showed that the rate of decreased level of consciousness, rate of cognitive or mental behavior abnormality, cerebrospinal fluid pressure, blood neutrophils, platelets, time until treatment initiation, neutrophil to lymphocyte ratio, platelet to lymphocyte ratio, and SII were significantly higher in patients with poor response to AE immunotherapy after 30 days than in patients with good response. Meanwhile, the blood lymphocyte counts and Glasgow Coma Scale (GCS) scores in patients with poor response were significantly lower than those in patients with good response (all p < 0.05), and multivariable binary logistic regression with backward stepwise method showed that decreased levels of consciousness, time until treatment initiation and SII were associated with poor response to immunotherapy. Moreover, the SII ≤ 863.3 group had lower rates of decreased consciousness levels, admission to the intensive care unit, and mechanical ventilation; lower cerebrospinal fluid pressure, blood neutrophil count, and platelet count; and higher blood lymphocyte count and GCS scores. The SII was associated with worsened disease severity and poor response to treatment after 30 days of the initially diagnosed AE, and patients with an SII > 863.3 were more likely to have poor response to immunotherapy.