bluuebunny/crossref_metadata_2025 huggingface dataset:
Transcriptomic Profiling of Primary Microglia: Effects of miR-19a-3p and miR-19b-3p on Microglia Activation
Authors: Sahebdel Faezeh , Zia Aliabbas , Quinta Hector Ramiro , Morse Leslie R. , Olson Julie K. , Battaglino Ricardo A.
Published: October 2024

Neuropathic pain resulting from spinal cord injury (SCI) is a significant secondary health issue affecting around 60% of individuals with SCI. After SCI, activation of microglia, the immune cells within the central nervous system, leads to neuroinflammation by producing pro-inflammatory cytokines and affects neuropathic pain. This interplay between inflammation and pain contributes to the persistent and intense pain experienced by many individuals with SCI. MicroRNAs (miRs) have been critical regulators of neuroinflammation. Previous research in our laboratory has revealed upregulation levels of circulating miR-19a and miR-19b in individuals with SCI with neuropathic pain compared to those without pain. In this study, we treated primary microglial cultures from mice with miR-19a and miR-19b for 24 h and conducted RNA sequencing analysis. Our results showed that miR-19a and miR-19b up- and downregulate different genes according to the volcano plots and the heatmaps. miR-19a and miR-19b regulate inflammation through distinct signaling pathways. The results showed that miR-19a promotes inflammation via toll-like receptor signaling, TNF signaling, and cytokine–cytokine receptor interactions, while miR-19b increases inflammatory responses through the PI3K-Akt signaling pathway, focal adhesion, and extracellular matrix receptor interactions. The protein–protein interaction (PPI) networks used the STRING database to identify transcription factors associated with genes up- or downregulated by miR-19a and miR-19b. Key transcription factors, such as STAT1, STAT2, and KLF4 for miR-19a, and Nr4a1, Nr4a2, and Nr4a3 for miR-19b, were identified and revealed their roles in regulating neuroinflammation. This study demonstrates that miR-19a and miR-19b modulate diverse patterns of gene expression, regulate inflammation, and induce inflammatory responses in microglia.
Polyphenols as the Main Compounds Influencing the Antioxidant Effect of Honey—A Review
Authors: Wilczyńska Aleksandra , Żak Natalia
Published: October 2024

Honey is one of the most valuable components of the human diet. It is considered to be a functional food with health-promoting properties. Honey has bactericidal and bacteriostatic effects; is used to treat wounds and ulcers; relieves stress; supports the treatment of diseases of the digestive and respiratory systems; improves kidney function; and aids in convalescence. The healing and prophylactic effects of honey are closely related to its chemical composition. According to the literature, honey contains over 300 substances belonging to various groups of chemical compounds, some with antioxidant activity, including vitamins and phenolic compounds, mainly flavonoids and phenolic acids. This article provides insight into honey’s chemical composition and its pro-health activities. The antioxidant properties of honey were prioritized.
Synergism or Antagonism: Do Arbuscular Mycorrhizal Fungi and Plant Growth-Promoting Rhizobacteria Work Together to Benefit Plants?
Authors: Savastano Noah , Bais Harsh
Published: October 2024

In agriculture, abiotic and biotic stress reduce yield by 51–82% and 10–16%, respectively. Applications of biological agents such as plant growth-promoting rhizobacteria (PGPR) and arbuscular mycorrhizal fungi (AMF) can improve plant growth. Applications of lone PGPR and AMF also help plants resist abiotic and biotic stressors. The reports for dual inoculation of AMF and PGPR to benefit plants and tackle stressors are largely unknown. It is speculated that PGPR colonization in plants enhances AMF infection during dual AMF and PGPR application, although increased AMF colonization does not always correlate with the increased benefits for the plant hosts. Further research is needed regarding molecular mechanisms of communication during dual inoculations, and dual-inoculation enhancement of induced systemic resistance under pathogen stress, to understand how dual inoculations can result in enhanced plant benefits. The influence of application timing of AMF and PGPR dual inoculations on mitigating abiotic and biotic stress is also not well understood. This review documents the factors that govern and modulate the dual application of AMF and PGPR for plant benefits against stress responses, specifically abiotic (drought) stress and stress from pathogen infection.
Enhancing Moisture Damage Resistance in Asphalt Concrete: The Role of Mix Variables, Hydrated Lime and Nanomaterials
Authors: Adwar Noor N. , Albayati Amjad H.
Published: October 2024

Moisture-induced damage is a serious problem that severely impairs asphaltic pavement and affects road serviceability. This study examined numerous variables in asphalt concrete mixtures to assess their impact on moisture damage resistance. Mix design parameters such as the asphalt content (AC) and aggregate passing sieve No. 4 (PNo. 4) were considered as variables during this study. Additionally, hydrated lime (HL) was utilized as a partial substitute for limestone dust (LS) filler at 1.5% by weight of the aggregate in asphalt concrete mixtures for the surface layer. This study also investigated the potential enhancement of traditional asphalt binders and mixtures by adding nano-additives, specifically nano-silica oxide (NS) and nano-titanium dioxide (NT), at rates ranging from 0% to 6% by weight of the asphalt binder. To quantify the moisture damage resistance of the asphalt concrete mixes, two types of laboratory tests were employed: the tensile strength ratio (TSR) and the index of retained strength (IRS). The former characterizes moisture damage using tensile strength, whereas the latter uses compression strength. The physical properties of the asphalt binder, such as its penetration, softening point, and ductility, were also evaluated to identify the effects of the nanomaterials. The results indicated that variations in the mix design variables significantly affected the moisture damage resistance of the asphalt concrete mixtures. The maximum improvement values were obtained at the optimum asphalt content (OAC) and PNo. 4 (mid-range + 6%) with TSR values of 80.45 and 82.46 and IRS values of 74.39 and 77.14, respectively. Modifying asphalt concrete mixtures with 1.5% HL resulted in improved moisture resistance compared with mixtures without HL (0% HL) at each PNo. 4 level, reaching superior performance at PNo. 4 (mid-range + 6%) by 4.58% and 3.96% in the TSR and IRS tests, respectively. Additionally, both NS and NT enhanced the physical properties of the asphalt binder, leading to substantial enhancements in asphalt concrete mixture performance against moisture damage. A 6% dosage of NS and NT showed the best performance, with NS performing slightly better than NT. TSR was increased by 14.72 and 11.55 and IRS by 15.60 and 12.75, respectively, with 6% NS and NT compared with mixtures without nanomaterials (0% NM).
The ANOCA/INOCA Dilemma Considering the 2024 ESC Guidelines on Chronic Coronary Syndromes
Authors: Sucato Vincenzo , Madaudo Cristina , Galassi Alfredo Ruggero
Published: October 2024

Cardiovascular disease remains a significant cause of morbidity and mortality worldwide, and its manifestations continue to pose a challenge in clinical practice [...]
Thermomechanical and Viscoelastic Characterization of Continuous GF/PETG Tape for Extreme Environment Applications
Authors: Colón Quintana José Luis , Tomlinson Scott , Lopez-Anido Roberto A.
Published: October 2024

The thermomechanical and viscoelastic properties of a glass fiber polyethylene terephthalate glycol (GF/PETG) continuous unidirectional (UD) tape were investigated using differential scanning calorimetry (DSC), thermomechanical analysis (TMA), and dynamic mechanical analysis (DMA). This study identified five operational conditions based on the Army Regulation 70-38 Standard. The DSC results revealed a glass transition temperature of 78.0 ± 0.3 °C, guiding the selection of temperatures for TMA and DMA tests. TMA provided the coefficient of thermal expansion in three principal directions, consistent with known values for PETG and GF materials. DMA tests, including strain sweep, temperature ramp, frequency sweep, creep, and stress relaxation, defined the material’s linear viscoelastic region and temperature-dependent properties. The frequency sweep indicated an increased modulus with rising frequency, identifying several natural frequency modes. Creep and stress relaxation tests showed time-dependent behavior, with strain increasing under higher loads and stress decreasing over time for all tested input values. Viscoelastic models fitted to the data yielded R2 values of 0.99, demonstrating good agreement. The study successfully measured thermomechanical and viscoelastic properties across various conditions, providing insights into how temperature influences the material’s mechanical response under extreme conditions.
Recycled Low Density Polyethylene Reinforced with Deverra tortuosa Vegetable Fibers
Authors: Zorgui Tahani , Ahmad Hibal , Romdhane Mehrez , Rodrigue Denis
Published: October 2024

In this work, natural fibers extracted from the medicinal aromatic plant Deverra tortuosa, with different sizes (S1 = 2 mm and S2 = 500 μm), were incorporated into recycled low density polyethylene (rLDPE) to produce sustainable biocomposites. Compounding was performed with different fiber concentrations (0 to 30% wt.) via twin-screw extrusion followed by injection molding. Based on the samples obtained, a comprehensive series of characterization was conducted, encompassing morphological and mechanical (flexural, tensile, hardness, and impact) properties. Additionally, thermal properties were assessed via differential scanning calorimetry (DSC), while Fourier transform infrared spectroscopy (FTIR) was used to elucidate potential chemical interactions and changes with processing. Across the range of conditions investigated, substantial improvements were observed in the rLDPE properties, in particular for the tensile modulus (23% for S1 and 104% for S2), flexural modulus (47% for S1 and 61% for S2), and flexural strength (31% for S1 and 65% for S2). Nevertheless, the tensile strength decreased (15% for S1 and 46% for S2) due to poor fiber–matrix interfacial adhesion. These preliminary results can be used for further development in sustainable packaging materials.
Fracture Mechanisms and Toughness in Polymer Nanocomposites: A Brief Review
Authors: Stern Theodor , Marom Gad
Published: October 2024

This article underlines the observation that, unlike the underperformance of nanocomposites in as far as their static mechanical properties of modulus and strength are concerned, fracture toughness exhibits exceptional behavior. This is attributed to the fact that fracture toughness expresses a measure of the energy absorbed in crack propagation, namely, the energy involved in creating new surface area, which, in turn, is controlled by a specific type of energy-dissipating interaction of the crack front with nanoparticles. This concise review focuses on two micromechanisms that are considered representative of energy dissipation due to their frequent presence in nanocomposites of both nanoparticles and nanofibers. Examples taken from recent relevant articles are presented to showcase fracture toughness improvements by nanoparticles.
The Evolutionary Game in Regulating Non-Agricultural Farmland Use within the Integrated Development of Rural Primary, Secondary, and Tertiary Industries
Authors: Cheng Liang , Huang Huimin , Sun Yong , Li Zhicui , Du Hongyan
Published: October 2024

Food security is a cornerstone of national development, and farmland protection and rationale are crucial for its assurance. However, integrating primary, secondary, and tertiary industries (IPSTI) in rural areas places significant pressure on farmland, threatening food security. This paper employs an evolutionary game model to explore how effective IPSTI can be achieved through stakeholder cooperation, ensuring rational farmland use under strict protection policies. The results reveal eight local equilibrium points in the game model, which can achieve asymptotic stability under varying conditions. Additionally, the behavioral parameters of local governments significantly impact their regulatory strategies for the non-agricultural use of farmland. When benefits increase, or penalties are strengthened, local government regulatory initiatives increase, constraining the behaviors of other participants. The village committee’s support strategy is influenced by benefits and costs, requiring a balance between promoting local economic development and minimizing non-agricultural use of farmland by enterprises. Enterprises’ decision-making primarily depends on the trade-off between the benefits and costs of non-agricultural use of farmland. The core findings of this study provide a crucial theoretical basis and decision support for formulating farmland protection policies and promoting industrial integration.
Synergistic Effects of Ternary Microbial Self-Healing Agent Comprising Bacillus pasteurii, Saccharomyces cerevisiae, and Bacillus mucilaginosus on Self-Healing Performance in Mortar
Authors: Wu Zhaoyun , Li Jiaxuan , Wang Tianlei , Zhang Lei , Peng Ben , Yue Changsheng
Published: October 2024

In order to prevent structural damage or high repair costs caused by concrete crack propagation, the use of microbial-induced CaCO3 precipitation to repair concrete cracks has been a hot topic in recent years. However, due to environmental constraints such as oxygen concentration, the width and depth of repaired cracks are seriously insufficient, which affects the further development of microbial self-healing agents. In this paper, a ternary microbial self-healing agent composed of different proportions of Bacillus pasteurii, Saccharomyces cerevisiae, and Bacillus mucilaginosus was designed, and its crack repair ability was evaluated. When the mixing ratio was 7:1:2, the cell concentration was the highest, the precipitation amount of CaCO3 was the highest, and the crystallinity of calcite crystal was the highest. Compared to the single microorganism, the mortar specimens with ternary microorganisms had the largest repair area (up to 100%) and the deepest repair depth (CaCO3 presents at 9–12 mm from the crack surface). This is because when the concrete breaks, all three microorganisms are activated by water, O2, and CO2. Saccharomyces cerevisiae and Bacillus mucilaginosus accelerated the growth of Bacillus pasteurii and more mineralized products; CaCO3 was rapidly formed and quickly filled on the crack surface. When CaCO3 seals the surface of the crack, the internal Saccharomyces cerevisiae and Bacillus mucilaginosus continue to play a role. Bacillus mucilaginosus can accelerate the dissolution of CO2 produced by the anaerobic fermentation of Saccharomyces cerevisiae and the hydrolysis of CO32−, thereby improving the repair of the crack depth direction.
Exceptional Strength–Ductility Combinations of a CoCrNi-Based Medium-Entropy Alloy via Short/Medium-Time Annealing after Hot-Rolling
Authors: Chen Yongan , Li Dazhao , Yan Zhijie , Bai Shaobin , Xie Ruofei , Sheng Jian , Zhang Jian , Li Shuai , Zhang Jinzhong
Published: October 2024

Strong yet ductile alloys have long been desired for industrial applications to enhance structural reliability. This work produced two (CoCrNi)93.5Al3Ti3C0.5 medium-entropy alloys with exceptional strength–ductility combinations, via short/medium (3 min/30 min) annealing times after hot-rolling. Three types of intergranular precipitates including MC, M23C6 carbides, and L12 phase were detected in both samples. Noticeably, the high-density of intragranular L12 precipitates were only found in the medium-time annealed sample. Upon inspection of the deformed substructure, it was revealed that the plane slip is the dominant deformation mechanism of both alloys. This is related to the lower stacking fault energy, higher lattice friction induced by the C solute, and slip-plane softening caused by intragranular dense L12 precipitates. Additionally, we noted that the stacking fault and twinning act as the mediated mechanisms in deformation of the short-time annealed alloy, while only the former mechanism was apparent in the medium-time annealed alloy. The inhibited twinning tendency can be attributed to the higher energy stacking faults and the increased critical twinning stress caused by intragranular dense L12 precipitates. Our present findings provide not only guidance for optimizing the mechanical properties of high/medium-entropy alloys, but also a fundamental understanding of deformation mechanisms.
Mechanism and Performance Control Methods of Sulfate Attack on Concrete: A Review
Authors: Zhang Chuanchuan , Li Julun , Yu Miao , Lu Yue , Liu Shizhong
Published: October 2024

For concrete structures in marine or groundwater environments, sulfate attack is a major factor contributing to the degradation of concrete performance. This paper analyzes the existing literature on the chemical reactions and physical crystallization effects of sulfate attack on cement-based materials, summarizing the degradation mechanisms of corroded concrete. Experiments have been conducted to study the performance evolution of concrete under sulfate attack, considering both external environmental factors and internal factors of the cement-based materials. External environmental factors, such as the temperature, humidity, concentration, and type of sulfate solutions, wet-dry cycles, freeze-thaw cycles, chloride coupling effects, and stray currents significantly impact sulfate attack on concrete. Internal factors, including internal sources of corrosion, the chemical composition of the cement, water-cement ratio, and the content of C-S-H gel and Ca(OH)2, influence the density and sulfate resistance of the cement-based materials. Additionally, five typical methods for enhancing the sulfate resistance of concrete are summarized. Finally, the paper identifies current challenges in the study of corroded concrete and proposes directions for future research.
Microstructure, Hardness and High-Temperature Corrosion Behaviors in Sulfur-Containing Environment of Laser Cladding Y2O3/IN625 Composite Coating
Authors: Li Yong , Zheng Hao , Chang Zhe , Liu Fuguang , Wang Yansong , Jian Yongxin
Published: October 2024

Water-cooled wall tubes are susceptible to high-temperature corrosion during service. Applying high-performance coatings via laser cladding on the tube surfaces can significantly enhance corrosion resistance and extend the service life of the tubes, providing substantial economic advantages. This paper prepared Y2O3/IN625 composite coating by means of high-speed laser cladding. Furthermore, the effects of Y2O3 addition on the microstructure evolution, hardness, as well as the high-temperature corrosion behaviors have been systematically investigated. The results show that Y2O3 addition can effectively refine the microstructure of the Inconel 625 coating, but the phase composition has little change. The coating’s hardness can also be improved by about 7.7%, reaching about 300 HV. Compared to Inconel 625 coating, the Y2O3-added composited coating shows superior high-temperature corrosion resistance, with the corrosion mass gain decreased by about 36.6%. The denser and tightly bonded Cr-rich oxides layer can be formed adjacent to the coating surface, which plays a predominant role in improving the coating corrosion resistance.
Effect of In Situ Polymerization of Super Absorbent Polymers on the Protection of Earthen Heritage Sites in Semi-Arid Regions
Authors: Zheng Yao , Liu Senbiao , Zhang Yifan , Du Zhilin , Li Miao , Luo Hongjie , Zhu Jianfeng
Published: October 2024

As precious cultural heritage, earthen sites are susceptible to various natural factors, leading to diverse forms of degradation. To protect earthen sites, the effects of super absorbent polymers (SAPs) on soil water retention, physical properties, and color compatibility at different concentrations were studied. After applying SAP treatment to an earthen site with different degrees of weathering, we drew the following conclusions. SAP-2 improved soil water retention capacity, increased soil water content, and slowed down the precipitation of soluble salts. At the same time, SAP-2 had the least effect on soil color difference and reduced the development of cracks by filling soil pores and enhancing the cohesion between soil particles, thus giving the earthen sites better weathering resistance. Therefore, the results provide a useful reference for the surface cracking of earthen sites in semi-arid areas and the degradation caused by flaking and block spalling.
Opalescence and Fluorescence of 46 Resin-Based Composites Exposed to Ultraviolet Light
Authors: Ghaffari Soheil , Gulati Anubhav , Price Richard Bengt
Published: October 2024

Identifying the boundary between the tooth and the resin-based composite (RBC) is difficult when replacing restorations. Ultraviolet (UV) light has been reported to assist the viewer by causing the RBC to fluoresce. Using a laboratory-grade fiberoptic spectrometer, 46 RBCs were exposed to UV light from the Woodpecker O-Star curing light. The opalescence and fluorescence were measured relative to a human tooth that contained just dentin and a tooth that contained both enamel and dentin. After these quantitative measurements, 10 RBCs with large differences in light emittance were compared qualitatively to assess their brightness when exposed to UV light compared to the dentin specimen and the specimen containing both enamel and dentin. It was found that, when exposed to UV light, some of the RBCs were less bright compared to the two samples of teeth used for comparison, but most were brighter; some were up to six times brighter. The filler appears to affect the opalescence peaks, while the resin appears to affect the fluorescence peaks. It was concluded that because RBCs emit very different levels of opalescence and fluorescence, UV light from the Woodpecker O-Star cannot be relied upon to detect all brands of RBC on the tooth. The opalescence and fluorescence can also be used to detect changes in the formulation of the RBC.
Electrostatically Interacting Wannier Qubits in Curved Space
Authors: Pomorski Krzysztof
Published: October 2024

A derivation of a tight-binding model from Schrödinger formalism for various topologies of position-based semiconductor qubits is presented in the case of static and time-dependent electric fields. The simplistic tight-binding model enables the description of single-electron devices at a large integration scale. The case of two electrostatically Wannier qubits (also known as position-based qubits) in a Schrödinger model is presented with omission of spin degrees of freedom. The concept of programmable quantum matter can be implemented in the chain of coupled semiconductor quantum dots. Highly integrated and developed cryogenic CMOS nanostructures can be mapped to coupled quantum dots, the connectivity of which can be controlled by a voltage applied across the transistor gates as well as using an external magnetic field. Using the anti-correlation principle arising from the Coulomb repulsion interaction between electrons, one can implement classical and quantum inverters (Classical/Quantum Swap Gate) and many other logical gates. The anti-correlation will be weakened due to the fact that the quantumness of the physical process brings about the coexistence of correlation and anti-correlation at the same time. One of the central results presented in this work relies on the appearance of dissipation-like processes and effective potential renormalization building effective barriers in both semiconductors and in superconductors between not bended nanowire regions both in classical and in quantum regimes. The presence of non-straight wire regions is also expressed by the geometrical dissipative quantum Aharonov–Bohm effect in superconductors/semiconductors when one obtains a complex value vector potential-like field. The existence of a Coulomb interaction provides a base for the physical description of an electrostatic Q-Swap gate with any topology using open-loop nanowires, with programmable functionality. We observe strong localization of the wavepacket due to nanowire bending. Therefore, it is not always necessary to build a barrier between two nanowires to obtain two quantum dot systems. On the other hand, the results can be mapped to the problem of an electron in curved space, so they can be expressed with a programmable position-dependent metric embedded in Schrödinger’s equation. The semiconductor quantum dot system is capable of mimicking curved space, providing a bridge between fundamental and applied science in the implementation of single-electron devices.
A Tomato Recognition and Rapid Sorting System Based on Improved YOLOv10
Authors: Liu Weirui , Wang Su , Gao Xingjun , Yang Hui
Published: October 2024

In order to address the issue of time-consuming, labor-intensive traditional industrial tomato sorting, this paper proposes a high-precision tomato recognition strategy and fast automatic grasping system. Firstly, the Swin Transformer module is integrated into YOLOv10 to reduce the resolution of each layer by half and double the number of channels, improving recognition accuracy. Then, the Simple Attention Module (SimAM) and the Efficient Multi-Scale Attention (EMA) attention mechanisms are added to achieve complete integration of features, and the Bi-level Routing Attention (BiFormer) is introduced for dynamic sparse attention and resource allocation. Finally, a lightweight detection head is added to YOLOv10 to improve the accuracy of tiny target detection. To complement the recognition system, a single-vertex and multi-crease (SVMC) origami soft gripper is employed for rapid adaptive grasping of identified objects through bistable deformation. This innovative system enables quick and accurate tomato grasping post-identification, showcasing significant potential for application in fruit and vegetable sorting operations.
Innovative Magnetic Gear Design Incorporating Electromagnetic Coils for Multiple Gear Ratios
Authors: Megahed Tamer F. , Gouda Eid Abdelbaki , Mansour Diaa-Eldin A. , El-Hussieny Haitham , Hameed Ibrahim A. , Fares Ahmed , Nassef Mohamed G. A.
Published: October 2024

In this study, a novel magnetic gear design is introduced. Unlike conventional magnetic gears that can only achieve a single gear ratio using permanent magnetic poles, the proposed design incorporates electromagnetic coils that can adapt to various control strategies, resulting in a multiple gear ratio for the same machine design. We selected a gear system with five gear ratios to validate the new design. The performance of the proposed design was compared with that of the conventional magnetic gear. While permanent magnet poles offer high torque transmission with a small volume, they cannot provide different gear ratios for the same configuration. Therefore, this work suggests using a single-gear machine based on a fixed number of electromagnetic coils to achieve different gear ratios. This research outlines the design steps, simulation process, and detailed analysis. The results demonstrate the effectiveness of the proposed design strategy, which can be potentially applied to wind turbines, transportation, and other scenarios with comparable success.
From Remote Sensing to Artificial Intelligence in Coral Reef Monitoring
Authors: Piñeros Victor J. , Reveles-Espinoza Alicia Maria , Monroy Jesús A.
Published: October 2024

This review comprehensively covers the development of viable unmanned underwater vehicles based on their technical capabilities, in particular those designed to conduct research exploration in underwater ecosystems and address environmental issues through analysis of coral reef vulnerability. The most important elements to be obtained are in situ data samples for analysis and characterization, supported by molecular biomarkers and marine ecology indicators. The following aspects are considered in this study: first, the geographic distribution of coral reefs for the study of marine ecology and molecular biological approaches for the detection of biomarkers to evaluate the vulnerability of coral reefs are detailed; then, the technologies currently available for the study of coral reefs are briefly described, ranging from large-scale capture to local-scale capture directly in the study region, taking advantage of remote sensing systems assisted by aerial technologies, marine vehicles, and artificial intelligence for the mapping, monitoring, and management of coral reefs as well as the characterization of their vulnerability; following this, existing marine vehicle technologies are generally explained, including a categorical description and an updated and highlighted list of innovative and robust marine vehicles that have been used in coral reef applications; the technical capabilities of such vehicle throughout the missions they have been subjected to are presented based on bibliographic references; finally, this review promotes multidisciplinary work to integrate the developments in the associated knowledge areas in order to obtain synergies in the face of challenges related to the massive scale of coral reef degradation worldwide.
Quantifying the Uncertainty of Reservoir Computing: Confidence Intervals for Time-Series Forecasting
Authors: Domingo Laia , Grande Mar , Borondo Florentino , Borondo Javier
Published: October 2024

Recently, reservoir computing (RC) has emerged as one of the most effective algorithms to model and forecast volatile and chaotic time series. In this paper, we aim to contribute to the understanding of the uncertainty associated with the predictions made by RC models and to propose a methodology to generate RC prediction intervals. As an illustration, we analyze the error distribution for the RC model when predicting the price time series of several agri-commodities. Results show that the error distributions are best modeled using a Normal Inverse Gaussian (NIG). In fact, NIG outperforms the Gaussian distribution, as the latter tends to overestimate the width of the confidence intervals. Hence, we propose a methodology where, in the first step, the RC generates a forecast for the time series and, in the second step, the confidence intervals are generated by combining the prediction and the fitted NIG distribution of the RC forecasting errors. Thus, by providing confidence intervals rather than single-point estimates, our approach offers a more comprehensive understanding of forecast uncertainty, enabling better risk assessment and more informed decision-making in business planning based on forecasted prices.
Anomaly Detection in Fractal Time Series with LSTM Autoencoders
Authors: Kirichenko Lyudmyla , Koval Yulia , Yakovlev Sergiy , Chumachenko Dmytro
Published: October 2024

This study explores the application of neural networks for anomaly detection in time series data exhibiting fractal properties, with a particular focus on changes in the Hurst exponent. The objective is to investigate whether changes in fractal properties can be identified by transitioning from the analysis of the original time series to the analysis of the sequence of Hurst exponent estimates. To this end, we employ an LSTM autoencoder neural network, demonstrating its effectiveness in detecting anomalies within synthetic fractal time series and real EEG signals by identifying deviations in the sequence of estimates. Whittle’s method was utilized for the precise estimation of the Hurst exponent, thereby enhancing the model’s ability to differentiate between normal and anomalous data. The findings underscore the potential of machine learning techniques for robust anomaly detection in complex datasets.
Multi-Strategy Enhanced Crested Porcupine Optimizer: CAPCPO
Authors: Liu Haijun , Zhou Rui , Zhong Xiaoyong , Yao Yuan , Shan Weifeng , Yuan Jing , Xiao Jian , Ma Yan , Zhang Kunpeng , Wang Zhibin
Published: October 2024

Metaheuristic algorithms are widely used in engineering problems due to their high efficiency and simplicity. However, engineering challenges often involve multiple control variables, which present significant obstacles for metaheuristic algorithms. The Crested Porcupine Optimizer (CPO) is a metaheuristic algorithm designed to address engineering problems, but it faces issues such as falling into a local optimum. To address these limitations, this article proposes three new strategies: composite Cauchy mutation strategy, adaptive dynamic adjustment strategy, and population mutation strategy. The three proposed strategies are then introduced into CPO to enhance its optimization capabilities. On three well-known test suites, the improved CPO (CAPCPO) outperforms 11 metaheuristic algorithms. Finally, comparative experiments on seven real-world engineering optimization problems demonstrate the advantages and potential of CAPCPO in solving complex problems. The multifaceted experimental results indicate that CAPCPO consistently achieves superior solutions in most cases.
Mitochondrial DNA Copy Number Is Associated with the Severity of Irritable Bowel Syndrome
Authors: Jung Soo-Jung , Lee Jae-Ho , Lim Ji-Yeon , Yang Yun-Yi
Published: October 2024

Background and Objectives: Irritable bowel syndrome (IBS), as a gastrointestinal disorder, presents with abdomen pain and alterations in the bowel habits. Its pathogenesis remains unclear. Here, we examined mitochondrial DNA copy number (mtCN) in IBS and its clinical value. Materials and Methods: mtCN was analyzed in 43 IBS patients using quantitative real-time polymerase chain reaction. Furthermore, data on the clinical characteristics of patients and symptom severity of IBS were collected, and their association with mtCN was analyzed. Results: mtCN was higher in patients with IBS (p = 0.008) and those with a drinking habit (p = 0.004). Smoking and the presence of a sleep partner showed a possible association with mtCN; however, it did not reach significance. The severity of IBS symptoms tended to positively correlate with mtCN (r = 0.279, p = 0.070). Conclusions: Overall, we demonstrated a potential association between mtCN and the clinicopathologic characteristics of patients with IBS. Further studies considering genetic and clinical factors are required.
Impact of Sarcopenia on Treatment Outcomes and Toxicity in Locally Advanced Rectal Cancer
Authors: Curcean Sebastian , Gherman Alexandra , Tipcu Alexandru , Fekete Zsolt , Muntean Alina-Simona , Curcean Andra , Craciun Rares , Stanciu Stefan , Irimie Alexandru
Published: October 2024

Background and Objectives: Sarcopenia, a condition characterized by muscle mass loss, is prevalent in up to 68% of rectal cancer patients and has been described as a negative prognostic factor, impacting overall survival and tumor response. While there are extensive data on rectal cancer globally, only a handful of studies have evaluated the role of sarcopenia in locally advanced rectal cancer (LARC). Our study aimed to investigate the relationship between sarcopenia, overall response rate, and toxicity in patients who underwent total neoadjuvant treatment (TNT) for LARC. Materials and Methods: We performed a retrospective study of patients with rectal cancer treated with TNT and surgery with curative intent between 2021 and 2023 at Prof. Dr. Ion Chiricuta Institute of Oncology, Cluj-Napoca. Sarcopenia was assessed on MRI images by measuring the psoas muscle area (PMA) at the level of the L4 vertebra before and after neoadjuvant therapy. The primary endpoints were the overall complete response rate (oCR) and acute toxicity. Results: This study included 50 patients with LARC. The oCR rate was 18% and was significantly associated with post-treatment sarcopenia (OR 0.08, p = 0.043). Patients who did not achieve a clinical or pathologic complete response had, on average, an 8% muscle loss during neoadjuvant therapy (p = 0.022). Cystitis and thrombocytopenia were significantly associated with post-treatment sarcopenia (p = 0.05 and p = 0.049). Conclusions: Sarcopenia and loss of psoas muscle during neoadjuvant therapy were negatively associated with tumor response in locally advanced rectal cancer. Thrombocytopenia and cystitis are more frequent in sarcopenic than non-sarcopenic patients undergoing neoadjuvant chemoradiation for rectal cancer.
Factors Associated with Health-Seeking Behavior in Indonesia: Evidence from the Indonesian Family Life Survey 2014
Authors: Hilmi Indah Laily , Alfian Sofa D. , Abdulah Rizky , Puspitasari Irma Melyani
Published: October 2024

Background and Objectives: Health-seeking behavior is a critical determinant of health outcomes, particularly in countries like Indonesia. Given the increasing burden of noncommunicable diseases, understanding the factors that influence health-seeking behavior in this context is essential for developing more accessible and effective public health strategies. This study aimed to identify various factors associated with health-seeking behavior among patients with chronic diseases across Indonesia, especially in formal facilities. Materials and Methods: This study used a cross-sectional research design, utilizing Indonesian Family Life Survey (IFLS)-5 data. The inclusion criteria included respondents aged 20–74 years old with at least one chronic disease based on self-reported data. Respondents who did not receive treatment, practiced self-medication, or provided incomplete data were excluded. We used multivariate logistic regression to identify factors associated with health-seeking behavior in formal facilities. Results: The results revealed that 80.7% (n = 1993) of the 2471 respondents sought treatment in formal facilities, whereas 19.3% (n = 478) opted for informal facilities. Respondents who were Bugis (OR 9.187, 95% CI 2.182–38.683; p = 0.002), retired (OR 2.966, 95% CI 1.233–7.135; p = 0.015), did not smoke (OR 1.604, 95% CI 1.126–2.285; p = 0.009), made less than IDR 1,500,000 a month (OR 1.466, 95% CI 1.174–1.831; p = 0.000), had to travel more than 3 km to reach a treatment facility (OR 1.847, 95% CI 1.41–2.42; p = 0.000), or had more than one comorbidity (OR 1.396, 95% CI; p = 0.01) were more likely to seek treatment at formal facilities. Conclusions: These findings are expected to provide recommendations for policymakers, healthcare providers, and researchers to contribute to the development of targeted interventions that can improve healthcare access and utilization, ultimately enhancing health outcomes and equity in Indonesia.
Impact of Polydeoxyribonucleotides on the Morphology, Viability, and Osteogenic Differentiation of Gingiva-Derived Stem Cell Spheroids
Authors: Lee Heera , Hwa Somyeong , Cho Sunga , Kim Ju-Hwan , Song Hye-Jung , Ko Youngkyung , Park Jun-Beom
Published: October 2024

Background and Objectives: Polydeoxyribonucleotides (PDRN), composed of DNA fragments derived from salmon DNA, is widely recognized for its regenerative properties. It has been extensively used in medical applications, such as dermatology and wound healing, due to its ability to enhance cellular metabolic activity, stimulate angiogenesis, and promote tissue regeneration. In the field of dentistry, PDRN has shown potential in promoting periodontal healing and bone regeneration. This study aims to investigate the effects of PDRN on the morphology, survival, and osteogenic differentiation of gingiva-derived stem cell spheroids, with a focus on its potential applications in tissue engineering and regenerative dentistry. Materials and Methods: Gingiva-derived mesenchymal stem cells were cultured and formed into spheroids using microwells. The cells were treated with varying concentrations of PDRN (0, 25, 50, 75, and 100 μg/mL) and cultivated in osteogenic media. Cell morphology was observed over seven days using an inverted microscope, and viability was assessed with Live/Dead Kit assays and Cell Counting Kit-8. Osteogenic differentiation was evaluated by measuring alkaline phosphatase activity and calcium deposition. The expression levels of osteogenic markers RUNX2 and COL1A1 were quantified using real-time polymerase chain reaction. RNA sequencing was performed to assess the gene expression profiles related to osteogenesis. Results: The results demonstrated that PDRN treatment had no significant effect on spheroid diameter or cellular viability during the observation period. However, a PDRN concentration of 75 μg/mL significantly enhanced calcium deposition by Day 14, suggesting increased mineralization. RUNX2 and COL1A1 mRNA expression levels varied with PDRN concentration, with the highest RUNX2 expression observed at 25 μg/mL and the highest COL1A1 expression at 75 μg/mL. RNA sequencing further confirmed the upregulation of genes involved in osteogenic differentiation, with enhanced expression of RUNX2 and COL1A1 in PDRN-treated gingiva-derived stem cell spheroids. Conclusions: In summary, PDRN did not significantly affect the viability or morphology of gingiva-derived stem cell spheroids but influenced their osteogenic differentiation and mineralization in a concentration-dependent manner. These findings suggest that PDRN may play a role in promoting osteogenic processes in tissue engineering and regenerative dentistry applications, with specific effects observed at different concentrations.
Navigating Safer Car Routes Based on Measured Car Accidents
Authors: Gandur Nazir L. , Ekwaro-Osire Stephen , Rasty Jahan , Parker Olin , Fernandes Guilherme
Published: October 2024

Car accidents, a major US public safety issue, demand precise analysis and predictive models for mitigation. This study asks the following question: Can the safest car routes across the US be determined? The paper analyzes historical data to forecast future accidents and calculates the safest route between two locations. The study builds a predictive model utilizing statistical analyses, data mining, and machine learning. A joint probability density function (PDF) is devised to calculate the safest route for risk modeling, factoring in latitude and longitude. The model quantifies accident probabilities in areas and travel routes. Additionally, the safest direction can be determined using the gradient of the joint PDF curve. The predictive model enables policymakers to allocate resources proactively. The safest route selection enables drivers to navigate safer areas and routes, which can reduce the number of accidents. Through its analysis and joint PDF model, this research enriches accident analysis and prevention engineering, potentially fostering safer US roads.
Increasing Ages of Inga punctata Tree Soils Facilitate Greater Fungal Community Abundance and Successional Development, and Efficiency of Microbial Organic Carbon Utilization
Authors: Eaton William D. , Hamilton Debra A.
Published: October 2024

Leguminous Inga trees are thought to enhance soil carbon (C) accumulation following reforestation, through mostly unknown mechanisms. This study amplified soil DNA using the ITS1F and ITS4 primers for PCR and Illumina MiSeq methods to identify fungal taxa, and traditional C analysis methods to evaluate how planted 4-, 8-, and 11-year-old Inga punctata trees affected soil fungal community compositions and C utilization patterns compared to old-growth I. punctata trees and an adjacent unplanted pasture within the same reforestation zone in Monteverde, Costa Rica. Along the tree age gradient, the planted I. punctata trees enhanced the tree soil C capture capacity, as indicated by increased levels of soil biomass C, Respiration, and efficiency of organic C use (with lower qCO2 values), and development of increasingly more abundant, stable, and successionally developed fungal communities, including those associated with the decomposition of complex organic C compounds. The level and strength of differences coincided with differences in the time of separation between the pasture and tree age or between the different tree ages. Fungal taxa were also identified as potential indicators of the early and late stages of soil recovery. Thus, planting I. punctata should be part of future reforestation strategies used in this region of the Monteverde Cloud Forest in Costa Rica.
Relaxivity Modulation of Gd-HPDO3A-like Complexes by Introducing Polar and Protic Peripheral Groups
Authors: Camorali Sara , Leone Loredana , Piscopo Laura , Tei Lorenzo
Published: October 2024

In the last three decades, high-relaxivity Magnetic Resonance Imaging (MRI) contrast agents (CAs) have been intensively sought, aiming at a reduction in the clinically injected dose while maintaining the safety of the CA and obtaining the same pathological information. Thus, four new Gd(III) complexes based on modified 10-(2-hydroxypropyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid (HP-DO3A) macrocyclic structure were designed and synthesized by introducing further polar and protic functional groups (amides, phosphonates, and diols) adjacent to the metal-coordinated hydroxyl group. A detailed 1H NMR relaxometric analysis allowed us to investigate the effect of these functional groups on the relaxivity, which showed a 20–60% increase (at 0.5 T, 298 K, and pH 7.4) with respect to that of clinically approved CAs. The contribution of the water molecules H-bonded to these peripheral functional groups on the relaxivity was evaluated in terms of the second sphere effect or prototropic exchange of labile protons.
Active Polysaccharide-Based Films Incorporated with Essential Oils for Extending the Shelf Life of Sliced Soft Bread
Authors: Noshirvani Nooshin , Le Coz Cédric , Gardrat Christian , Ghanbarzadeh Babak , Coma Véronique
Published: October 2024

Active, fully biobased film-forming dispersions (FFDs) with highly promising results for sliced soft bread preservation were successfully elaborated from carboxymethyl cellulose (CMC) and chitosan (CH) using a simple method based on pH adjustments. They consisted of the association of polysaccharides and oleic acid (OL) added with cinnamon (CEO) or ginger (GEO) essential oils. The chemical compositions of the commercial essential oils were first determined via GC/MS, with less than 3% of compounds unidentified. The films obtained from FFDs were characterized by SEM, FTIR and DSC, indicating specific microstructures and some interactions between essential oils and the polymer matrix. CEO-based films exhibited higher antioxidant properties and a lower minimal inhibitory concentration in terms of antifungal properties. From experiments on sliced soft bread, the ginger-based films could increase the shelf life up to 20 days longer than that of the control. Even more promising, cinnamon-based films led to complete fungal inhibition in bread slices that was maintained beyond 60 days. Enumeration of the yeasts and molds for the FFD-coated breads revealed complete inhibition even after 15 days of storage with the FFDs containing the highest concentration of CEO.