bluuebunny/crossref_metadata_2025 huggingface dataset:
GC-MS and SPME Techniques Highlighted Contrasting Chemical Behaviour in the Water Extractives of Modified Castanea sativa Mill. and Fagus sylvatica L. Wood
Authors: Mecca Marisabel , Todaro Luigi , Lo Giudice Valentina , Lovaglio Teresa , D’Auria Maurizio
Published: July 2021

The sweet chestnut (Castanea sativa Mill.) and European beech (Fagus sylvatica L.) are wood species largely present in the European forest area. The composition and relative variation of the secondary metabolites of chestnut and European beech wood under thermal effect is a little-explored area. The wood material was thermally modified at 170 °C for 3 h using a thermo-vacuum technology. Raw and modified wood extracts were obtained with aqueous extraction techniques in an autoclave, subsequently lyophilized, solubilized in ethyl acetate, and determined by Gas Chromatographic-Mass Spectrometric Analyses (GC-MS). In addition, the volatile compounds were determined by Solid-Phase Micro Extraction (SPME) analyses. As a general statement, the extraction in an autoclave produced a higher number of compounds in the modified chestnut and beech wood compared to unmodified wood material. Beech wood showed low degradation in the compounds after modification. Notably, squalene and ar-tumerone were the main bioactive compounds present in beech wood extractives. Chestnut, conversely, showed a greater degradation after thermo-modification. However, a reduction in chemical compounds in the modified samples was also observed. In this case, the main biologically active compounds detected only in the chestnut control samples were apocynin and ar-tumerone. The recovery of this residual wood material, before energy consumption, could provide a sustainable and environmentally friendly means of obtaining natural chemicals suitable for various industrial applications.
Do Review Papers on Bird–Vegetation Relationships Provide Actionable Information to Forest Managers in the Eastern United States?
Authors: Lott Casey A. , Akresh Michael E. , Costanzo Bridgett E. , D’Amato Anthony W. , Duan Shengwu , Fiss Cameron J. , Fraser Jacob S. , He Hong S. , King David I. , McNeil Darin J. , Stoleson Scott H. , Yamasaki Mariko , Larkin Jeffery L.
Published: July 2021

Forest management planning requires the specification of measurable objectives as desired future conditions at spatial extents ranging from stands to landscapes and temporal extents ranging from a single growing season to several centuries. Effective implementation of forest management requires understanding current conditions and constraints well enough to apply the appropriate silvicultural strategies to produce desired future conditions, often for multiple objectives, at varying spatial and temporal extents. We administered an online survey to forest managers in the eastern US to better understand how wildlife scientists could best provide information to help meet wildlife-related habitat objectives. We then examined more than 1000 review papers on bird–vegetation relationships in the eastern US compiled during a systematic review of the primary literature to see how well this evidence-base meets the information needs of forest managers. We identified two main areas where wildlife scientists could increase the relevance and applicability of their research. First, forest managers want descriptions of wildlife species–vegetation relationships using the operational metrics of forest management (forest type, tree species composition, basal area, tree density, stocking rates, etc.) summarized at the operational spatial units of forest management (stands, compartments, and forests). Second, forest managers want information about how to provide wildlife habitats for many different species with varied habitat needs across temporal extents related to the ecological processes of succession after harvest or natural disturbance (1–2 decades) or even longer periods of stand development. We provide examples of review papers that meet these information needs of forest managers and topic-specific bibliographies of additional review papers that may contain actionable information for foresters who wish to meet wildlife management objectives. We suggest that wildlife scientists become more familiar with the extensive grey literature on forest bird–vegetation relationships and forest management that is available in natural resource management agency reports. We also suggest that wildlife scientists could reconsider everything from the questions they ask, the metrics they report on, and the way they allocate samples in time and space, to provide more relevant and actionable information to forest managers.
Is the Reproduction Capacity of Pinus brutia Stands 20 Years after Wildfire Efficient to Secure Forest Restoration in the Case of a Fire Re-Occurrence?
Authors: Ganatsas Petros , Giannakaki Maria , Gouvas Alexandros , Tsakaldimi Marianthi
Published: July 2021

A critical issue in effective post-fire regeneration and re-establishment of a burnt forest is the stage concerning the burned stands. When stands are mature and produce seeds, the regeneration can theoretically be achieved, while in the case of immature stands, they may not be able to produce enough quantities of germinable seeds that can secure stand re-establishment. It is estimated that a period of 15–16 years after fire is needed for enough seed production to achieve natural forest re-establishment. This study aimed at the estimation of the real reproduction capacity of Pinus brutia stands 20 years after a wildfire, in an area of northern Greece. The study focused on stand structure, cone production and their morphological characteristics, the morphological characteristics of the produced seed as well as quality of produced seeds. Results analyses showed that despite the young age of the stands, they are quite productive, producing a high number of cones per ha, with a high percentage of trees bearing cones, but with a low number of cones per tree (11.0 cones). An average number of 58.0 fully developed seeds per cone were found, which corresponds to a total number of 620,136 seeds per ha. Considering that the average rate of seed germination is 25.6% (at laboratory conditions), and the theoretical possibility for establishing viable seedling under ambient conditions, these data give an estimation of 1587.5 of one-year old seedlings to recruit the burnt area, in the case of a fire event, if all other parameters remain favorable. These data confirmed the hypothesis that a period of 15–16 years interval between two fires is enough for a burnt serotinous pine forest to reach in such a reproductive stage, able to secure its renewal if a fire occurs. This should be greatly considered in forest fire protection and management plans, which should aim to effectively protect young post-fire forests at least to the age of 15–16 years.
Digital Manufacturing Challenges Education—SmartLab Concept as a Concrete Example in Tackling These Challenges
Authors: Tihinen Maarit , Pikkarainen Ari , Joutsenvaara Jukka
Published: July 2021

Digitalization is boosting the manufacturing industry’s shift to smart manufacturing systems, which will efficiently utilize the potential of new technologies for their business outcomes and value. However, the literature shows that manufacturing companies have implemented very little digital technology due to a lack of the required knowledge and competences. Increasingly, interconnected, digitalized, and complex processes lead to new skill requirements in companies and thereafter also of their workforce’s training needs to respond to the smart manufacturing’s new great expectations. The article provides concrete examples of tackling challenges in education arising from digital manufacturing. The case study introduced in this article concerns the additive manufacturing (AM) method, which is expected to give rise to significant changes in various industrial fields, including digital manufacturing. Advances in digital manufacturing requires skilled professionals who are aware of the possibilities and potential of the latest technology. Education therefore needs to be developed. This article points out that the built learning and development environment, SmartLab, supports multidisciplinary approaches and close collaboration between several stakeholders like companies, engineering education courses, students, and RDI actors. The SmartLab concept is thus also expected to provide a remarkable competitive advantage for business in the region.
Applying Computational Fluid Dynamics in the Development of Smart Ripening Rooms for Traditional Cheeses
Authors: Alvarenga Nuno , Martins João , Caeiro José , Garcia João , Pássaro João , Coelho Luis , Santos Maria Teresa , Lampreia Célia , Martins António , Dias João
Published: July 2021

Traditional ewe’s cheese producers face certain challenges caused by fluctuating environmental parameters inside the ripening room, which lead to lack of homogeneity in the final product. The present research discusses the application of computer fluid dynamics for simulating the distribution of environmental parameters, predicting the airflow pattern, and identifying critical areas where such parameters could cause reduced cheese quality. A new monitoring system was developed including presence sensors, temperature and humidity dataloggers, pneumatic actuators, microcontrollers, and microcomputers connected remotely for control, data visualization, and processing. The validation of the computer simulation and monitoring system was made with a batch of 40 ewe’s cheeses distributed in three different zones inside a prototype ripening room and ripened for 35 days. At 35 days, a physical, chemical, and microbiological characterization of cheeses was made for evaluation of the influence of environmental conditions on cheese quality. The comparison between simulated and local measurements showed close agreement, especially concerning air velocity inside the stacks of cheese. The results of Pearson’s correlation analysis and PCA concluded that temperature affected the appearance of the rind, hardness, number and area occupied by holes. Humidity affected aw and mFeret. Air velocity affected pH and the circularity of gas holes.
Characterization of Bacillus cereus AFA01 Capable of Degrading Gluten and Celiac-Immunotoxic Peptides
Authors: Lu Jun , Wu Yong , Yuan Juanli , Yuan Jin , Wang Zhongliang , Gao Jinyan , Chen Hongbing
Published: July 2021

Wheat gluten elicits a pro-inflammatory immune response in patients with celiac disease. The only effective therapy for this disease is a life-long gluten-free diet. Gluten detoxification using glutenases is an alternative approach. A key step is to identify useful glutenases or glutenase-producing organisms. This study investigated the gluten-degrading activity of three Bacillus cereus strains using gluten, gliadin, and highly immunotoxic 33- and 13-mer gliadin peptides. The strain AFA01 was grown on four culture media for obtaining the optimum gluten degradation. Complete genome sequencing was performed to predict genes of enzymes with potential glutenase activity. The results showed that the three B. cereus strains can hydrolyze gluten, immunotoxic peptides, and gliadin even at pH 2.0. AFA01 was the most effective strain in degrading the 33-mer peptide into fractions containing less than nine amino acid residues, the minimum peptide to induce celiac responses. Moreover, growth on starch casein broth promoted AFA01 to degrade immunotoxic peptides. PepP, PepX, and PepI may be responsible for the hydrolysis of immunotoxic peptides. On the basis of the potential of gluten degradation, AFA01 or its derived enzymes may be the best option for further research regarding the elimination of gluten toxicity.
Analysis of Copper(II), Cobalt(II) and Iron(III) Sorption in Binary and Ternary Systems by Chitosan-Based Composite Sponges Obtained by Ice-Segregation Approach
Authors: Dinu Maria Valentina , Humelnicu Doina , Lazar Maria Marinela
Published: July 2021

With the intensive industrial activity worldwide, water pollution by heavy metal ions (HMIs) has become a serious issue that requires strict and careful monitoring, as they are extremely toxic and can cause serious hazards to the environment and human health. Thus, the effective and efficient removal of HMIs still remains a challenge that needs to be solved. In this context, copper(II), cobalt(II) and iron(III) sorption by chitosan (CS)-based composite sponges was systematically investigated in binary and ternary systems. The composites sponges, formed into beads, consisting of ethylenediaminetetraacetic acid (EDTA)- or diethylenetriaminepentaacetic acid (DTPA)-functionalized CS, entrapping a natural zeolite (Z), were prepared through an ice-segregation technique. The HMI sorption performance of these cryogenically structured composite materials was assessed through batch experiments. The HMI sorption capacities of CSZ-EDTA and CSZ-DTPA composite sponges were compared to those of unmodified sorbents. The Fe(III) ions were mainly taken up when they were in two-component mixtures with Co(II) ions at pH 4, whereas Cu(II) ions were preferred when they were in two-component mixtures with Co(II) ions at pH 6. The recycling studies indicated almost unchanged removal efficiency for all CS-based composite sorbents even after the fifth cycle of sorption/desorption, supporting their remarkable chemical stability and recommending them for the treatment of HMI-containing wastewaters.
Biochemical and Molecular Effects Induced by Triacontanol in Acquired Tolerance of Rice to Drought Stress
Authors: Alharbi Basmah M. , Abdulmajeed Awatif Mahfouz , Hassan Heba
Published: July 2021

To assess the effect of triacontanol (TRIA) on rice plants grown under normal or drought conditions, rice seeds were presoaked in TRIA (35 ppm) for two hours. After 20 days of sowing, rice seedlings developed from TRIA-treated or untreated seeds were subjected to drought stress. After 10 days of plant exposure to drought stress, data of major growth attributes and the content of photosynthetic pigments were recorded. Moreover, the effect of drought stress on stomatal conductance and the photochemical efficiency of PSII (Fv/Fm) were followed. The data obtained indicated that the species of rice (Oryza sativa L.) cultivar Giza 177 under investigation was sensitive to drought stress where there were significant decreases in the fresh and dry weights of shoots and roots and in stomatal conductance, as well as in the content of chlorophyll a, chlorophyll b, and carotenoids. Seed priming with TRIA enhanced both growth and acquired plant tolerance to drought stress. Thus, TRIA via the enhancement of stomatal conductance through the regulation of stomatal closure, the rate of water loss, ABA metabolism, the accumulation of osmolytes, and the regulation of aquaporins genes improved the water status of plants grown under water scarcity. Moreover, TRIA via increasing the content of free amino acids and sugars under drought stress may increase the chance of plant tissues to retain more water under scarcity conditions.
Plant Variety Protection: Current Practices and Insights
Authors: Yu Ju-Kyung , Chung Yong-Suk
Published: July 2021

Breeders persistently supply farmers with the best varieties in order to exceed consumer demand through plant-breeding processes that are resource-intensive. In order to motivate continuous innovation in variety development, a system needs to provide incentives for plant breeders to develop superior varieties, for example, exclusive ownership to produce and market those varieties. The most common system is the acquisition of intellectual property protection through plant variety protection, also known as the breeder’s right. Most countries have adopted the system established by the International Union for the Protection of New Varieties of Plants (UPOV). To be granted plant variety protection, the variety should prove to be unique by meeting three requirements: distinctness, uniformity, and stability. This review summarizes (1) the plant variety protection via UPOV convention, (2) technical methods for distinctness, uniformity, and stability testing via phenotype, molecular markers, and sequencing as well as their challenges and potentiality, and (3) additional discussions in essentially derived variety, value for cultivation and use testing, and open source seed initiative.
Geochemical Trends Reflecting Hydrocarbon Generation, Migration and Accumulation in Unconventional Reservoirs Based on Pyrolysis Data (on the Example of the Bazhenov Formation)
Authors: Spasennykh Mikhail , Maglevannaia Polina , Kozlova Elena , Bulatov Timur , Leushina Evgeniya , Morozov Nikita
Published: July 2021

The current study is devoted to the determination and interpretation of geochemical trends reflecting hydrocarbon generation, migration and accumulation in unconventional reservoirs; the study is performed on the Bazhenov shale rock formation (Western Siberia, Russia). Results are based on more than 3000 Rock-Eval analyses of the samples from 34 wells drilled in the central part of the West Siberian petroleum basin, which is characterized by common marine sedimentation environments. Pyrolysis studies were carried out before and after the extraction of rocks by organic solvent. As a result, we have improved the accuracy of kerogen content and maturity determination and complemented the standard set of pyrolysis parameters with the content of heavy fraction of hydrocarbons. The data obtained for the wells from areas of different organic matter maturity was summarized in the form of cross-plots and diagrams reflecting geochemical evolution of the source rocks from the beginning to the end of the oil window. Interpretation of the obtained results revealed quantitative trends in the changes of generation potential, amount, and composition of generated hydrocarbons in rocks at different stages of oil generation process. The analysis of geochemical trends allowed us to improve approaches for the productivity evaluation of the formation and study the effect of organic matter maturity on distribution of productive intervals of different types.
Infection Spread, Recovery, and Fatality from Coronavirus in Different Provinces of Saudi Arabia
Authors: Alharbi Mohammed Muberek , Rabbani Syed Imam , Asdaq Syed Mohammed Basheeruddin , Alamri Abdulhakeem S. , Alsanie Walaa F. , Alhomrani Majid , Mohzari Yahya , Alrashed Ahmed , Bamogaddam Reem Faisal , Alajlan Saleh Ahmad , Alharbi Mansour A. , Aldhawyan Norah N. , Najmi Saeed A.
Published: July 2021

The World Health Organization (WHO) announced COVID-19, a novel coronavirus outbreak, as a pandemic in 2020. In the month of February 2020, the disease began to spread through the Middle East. The first case of COVID-19 in the Kingdom of Saudi Arabia was identified in March 2020, and it is now one of the region’s most affected countries. Analyzing the disease’s propagation pattern may aid in the development of pandemic-fighting strategies. This study aims to analyze the trend of COVID-19’s spread, its recovery, and mortality in the Kingdom of Saudi Arabia (KSA). Two to three major cities from the 13 provinces of the country were chosen, and the rate of infection recovery was recorded from the first month until the number of confirmed cases showed a decline. The data published on the official Ministry of Health website were recorded on an Excel sheet, graphically represented as figures to indicate the pattern of spread. According to the study’s findings, COVID-19 positive cases were discovered in the majority of provinces as early as March 2020. The province of Makkah had the largest number of COVID-19 positive cases (30.7%), followed by Riyadh (23%). The province of Al Jowf had the lowest number of COVID-19 cases (0.3%). Tabuk province had the highest rate of recovery (97.8%), followed by the Northern Border Province (96.7%). Makkah province had the highest mortality rate (2.6%), followed by Al Jawf province (2.4%). The peak case–fatality ratio was recorded in August and September. The highest number of tests to detect the COVID-19 was performed in the month of July, and the highest percentage of positive cases was detected in June (19.55%). All the provinces from the month of September 2020 showed a progressive decline in the number of confirmed COVID-19 cases. According to this study, COVID-19 infection was found in the majority of Saudi Arabian provinces in March 2020, with a peak in June–July 2020. Considering the climatic and demographic characteristics of the region, specific modalities need to be adopted in collaboration with international guidelines to defeat the COVID-19 pandemic.
Hermeneutic Phenomenological Understanding of the Inner Journey of Templestay
Authors: Hwang Jiyoung , Park Hyo-Yeun
Published: July 2021

This study delineated the templestay experience in the context of the authenticity of tourism. To understand the phenomenon of templestay experience, the study applied the hermeneutic phenomenology method. Furthermore, the researcher tried to approach the experience with an open perspective in observing essential meanings and lived phenomena. From the hermeneutic guide, the study discovered “structure of perceptions (situation, emotion, thought, action),” “perceptual experiences (rational experience, sentimental experience, judgmental experience, experimental experience),” and “experiential authenticity (superficial authenticity, situational authenticity, relational authenticity, space-time authenticity, existential authenticity)” from the lived templestay experience and developed a “descriptive model of the integrative phenomenon of templestay experience.” This study suggests the possibility of discovering new phenomena by expanding the boundaries of the perceptions of the authentic experience of tourism.
Perspectives and Attitudes of Patients with COVID-19 toward a Telerehabilitation Programme: A Qualitative Study
Authors: Bernal-Utrera Carlos , Anarte-Lazo Ernesto , De-La-Barrera-Aranda Elena , Fernandez-Bueno Laura , Saavedra-Hernandez Manuel , Gonzalez-Gerez Juan Jose , Serrera-Figallo Maria Angeles , Rodriguez-Blanco Cleofas
Published: July 2021

The total isolation of patients with coronavirus disease 2019 (COVID-19) requires non-face-to-face medical assistance. There is evidence of the efficacy of home treatments with exercises in patients with respiratory disorders which could become the therapeutic method of choice for the treatment and supervision of patients isolated due to infection during home confinement. This study’s objective was to analyse the experience and opinions of isolated patients with COVID-19 included in a programme of telerehabilitation exercises for 14 days and it is intended to reflect, from a qualitative point of view, the viability and usefulness of telerehabilitation tools in the management of these patients. Twenty-five participants of a telerehabilitation programme were interviewed by telephone through semi-structured interviews, following a positivist and objective model. The data were categorised and analysed through NVIVO qualitative analysis software. The information obtained was classified into four main topics (telerehabilitation programme, perception of clinical benefit, psychological aspects and level of health care) and six subtopics (technical aspects, communication, improvement aspects, exercise plan, motivation and applicability to public health systems). The telerehabilitation programme established in patients confined by COVID-19 is very well received, without considerable technical difficulties and generates physical and psychological improvements. Patients highlight the importance of applying this type of programme in public health systems.
Consumption of Alcohol, Cannabis, and Tobacco in a Cohort of Adolescents before and during COVID-19 Confinement
Authors: Rogés Judit , Bosque-Prous Marina , Colom Joan , Folch Cinta , Barón-Garcia Tivy , González-Casals Helena , Fernández Esteve , Espelt Albert
Published: July 2021

The aim of this study was to identify changes in the hazardous consumption of alcohol, tobacco, and cannabis, due to the COVID-19 lockdown in 2020 in a cohort of schooled adolescents from Central Catalonia. We also analyzed the effect of the individual and social factors on risky consumption during confinement. This longitudinal study involved a subsample of 303 adolescents aged 14–18 years, who were attending 4th year of compulsory secondary education (ESO), 2nd year of college preparation (baccalaureate), or Vocational and Educational Training (VET). We collected data before COVID-19 lockdown (October 2019–February 2020) and 2 months after the lockdown ended. We estimated the prevalence of risky substance use in the sample at baseline for each independent variable. We used Poisson regression models with robust variance to compute the Cumulative Incidence (CI) and Relative Risk (RR), with their respective 95% confidence interval. We found that VET students had a significantly (p < 0.05) higher risk of substance use: binge drinking (RR = 3.21 (95%CI: 1.00–10.34)); hazardous drinking of alcohol (RR = 3.75 (95%CI: 1.12–12.54)), hazardous consumption of cannabis (RR = 3.75 (95%CI: 0.65–21.59)) and daily smoking of tobacco (RR = 4.82 (95%CI: 1.74–13.39)). The results showed a general trend of reduction of consumption during COVID-19 confinement period. This study suggests that VET students were more likely to engage in hazardous drinking of alcohol and daily smoking of tobacco. No statistically significant differences were found for the other age groups and variables.
Asymmetric Power Relations in Gynaecological Consultations for Cervical Cancer Prevention: Biomedical and Gender Issues
Authors: Freijomil-Vázquez Carla , Gastaldo Denise , Coronado Carmen , Movilla-Fernández María-Jesús
Published: July 2021

A generic qualitative research, using a poststructuralist feminist perspective, was conducted in a Spanish gynaecology unit with the following aims: (a) to analyse how asymmetric power relations in relation to biomedical knowledge and gender shape the medical encounters between gynaecologists and women diagnosed with cervical intraepithelial neoplasia and (b) to explore the cognitive, moral, and emotional responses expressed by patients. A total of 21 women diagnosed with cervical intraepithelial neoplasia were recruited through purposive sampling. Semi-structured interviews were recorded and transcribed, and a thematic analysis was carried out. Two major themes were identified: (a) gendered relations in cervical intraepithelial neoplasia medical encounters are based on hidden, judgmental moral assumptions, making women feel irresponsible and blamed for contracting the human papillomavirus infection; (b) biomedical power is based on the positivist assumption of a single truth (scientific knowledge), creating asymmetric relations rendering women ignorant and infantilised. Women reacted vehemently during the interviews, revealing a nexus of cognitive, moral, and emotional reactions. In medical encounters for management of cervical intraepithelial neoplasia, patients feel they are being morally judged and given limited information, generating emotional distress. Healthcare professionals should question whether their practices are based on stereotypical gender assumptions which lead to power asymmetries during encounters.
Music Tempo: A Tool for Regulating Walking Cadence and Physical Activity Intensity in Overweight Adults?
Authors: Faulkner Maria , McNeilly Andrea , Davison Gareth , Rowe David , Hewitt Allan , Nevill Alan , Duly Ellie , Trinick Tom , Murphy Marie
Published: July 2021

This study investigated if music tempo can prompt a desired walking cadence, and if music can provide a stimulus to regulate physical activity intensity in a longitudinal physical activity intervention with free-living adults. Overweight adults (n = 37; 94.26 ± 17.11 kg; 49.63 ± 12.37 years) were randomly assigned to an intervention (IG, n = 17) or usual care group (UC, n = 20) as part of a novel nine-month walking intervention. IG participants walked to self-selected music with a predetermined tempo and received a behavioural change support programme. At baseline, four-, six- and nine-months participants were asked to walk around an elliptical track at their habitual pace (0–2 min) and then in time to a predetermined tempo (2–8 min) designed to elicit moderate intensity. Cadence response (steps/min) was assessed and intensity (heart rate (bpm) recorded using wireless telemetry. A repeated measures general linear model (GLM) examined differences between groups over time (p < 0.05). All data is presented as means ± SD. At each assessment point both groups displayed an immediate cadence adjustment in response to music tempo (p < 0.01) i.e., habitual cadence vs. 3 METs target cadence (p < 0.05) and 3 METs target cadence vs. 5 METs target cadence (p < 0.05). Additionally, IG participants displayed an increased habitual cadence (0–2 min) at each assessment point (p < 0.05; 110 ± 9, 121.80 ± 7.5, 121.46 ± 10, 121.93 ± 7 steps/min respectively). UC participant’s habitual cadence was unchanged from 0–9 months (p > 0.05; 120 ± 10, 116 ± 13, 119 ± 12 and 119 ± 9 steps/min respectively). Music tempo may be a useful regulatory tool to prompt the free-living individual to reach an appropriate stride rate to achieve a walking pace that is at least moderate intensity. It also appears that results may be trainable as throughout the study an increased habitual walking cadence was observed, in the absence of music.
Emotional Exhaustion, Depersonalization, and Mental Health in Nurses from Huelva: A Cross-Cutting Study during the SARS-CoV-2 Pandemic
Authors: Gago-Valiente Francisco-Javier , Mendoza-Sierra María-Isabel , Moreno-Sánchez Emilia , Arbinaga Félix , Segura-Camacho Adrián
Published: July 2021

Currently, healthcare professionals are particularly vulnerable to the impact of the SARS-CoV-2pandemic since they directly deal with patients suffering from this disease and are in the first line of fire, which increases their risk of contagion. This research examines the prevalence of emotional exhaustion, depersonalization, and possible non-psychotic psychiatric disorders in 48 male and 270 female nursing professionals of Huelva during the COVID-19 pandemic. To this end, we analyzed the relationship between these dependent variables and considered various sociodemographic variables. The nursing staff of public hospitals in Huelva who have had contact with cases of SARS-CoV-2 in their work environment showed a poorer state of mental health than that of others of this same professional category who have not had contact with this type of situation.
A Latent Class Analysis of Health-Related Quality of Life in Korean Older Adults
Authors: Choi Eun-Hi , Kang Mi-Jung , Lee Hyun-Jin , Yun Mi-Soon
Published: July 2021

The present study aimed to confirm latent classes in health-related quality of life (HRQOL) in older adults and investigate the characteristics of participants in each class. It aimed to provide basic data to develop interventions for each quality-of-life class by analysing the predictors of each class. Secondary data from a community health survey in G province since 2019 found a total of 41,872 participants. Of them, 9027 were 65 years or older and residing in G Province in 2019, participated in this study. Mplus 8.5 was used to conduct a latent class analysis of five domains of HRQOL. Four latent classes in the HRQOL of older adults, namely, stable type, physical disability type, emotional disability type, and crisis type were found. Certain variables predicted these classes. Based on the findings of the present study, training on functional mobility and balance to prevent falls in older populations and individualised programmes to promote mental health in them should be provided. Moreover, policies should increase medical accessibility and provide social support for older people with low-incomes. Additionally, since physical, psychological, and social health in older adults are inter-connected, a comprehensive care plan is needed to improve their HRQOL.
Investigating the Effective Factors of Using Personal Protective Equipment from the Perspective of Nurses Caring for COVID-19 Patients: A Cross-Sectional Study
Authors: Mokhtari Razieh , Safdari Ali , Hekmatpou Davood , Sahebi Ali , Moayedi Siamak , Torres Mercedes , Golitaleb Mohamad
Published: July 2021

Considering the importance of appropriate personal protective equipment (PPE) for preventing COVID-19 transmission, the aim of this study was to investigate the factors affecting the use of PPE from the perspective of the nurses caring for COVID-19 patients. This descriptive cross-sectional study surveyed 240 nurses working in the central COVID-19 hospitals of Arak, Iran. Nurses were enrolled in the study by a convenience sampling method. The data collection tool was a validated questionnaire. Data were analyzed by SPSS 16 software using descriptive statistics, analysis of variance (ANOVA), and independent sample t-test. Environmental (4.24 ± 0.45), personal (4.16 ± 0.42), and organizational (4.04 ± 0.50) factors all contribute significantly to nursing attitudes about PPE use (p < 0.05). The average score, combining all identified factors, was 4.15 ± 0.31. The most influential factor contributing to appropriate use of PPE was environmental, while the least impactful parameters were related to rules and regulations. Environmental factors have the greatest impact on the use of PPE from the perspective of the nurses caring for patients with COVID-19. Managers and healthcare organizations should provide appropriate and adequate PPE to nurses, educate them on proper use, and monitor the process to resolve barriers.
Translational Research in Cancer Screening: Long-Term Population-Action Bridges to Diffuse Adherence
Authors: Hagoel Lea , Rennert Gad , Neter Efrat
Published: July 2021

The population-level implementation of innovative, evidence-based medical recommendations for adopting health-behaviors depends on the last link in the translation chain: the users. “User-friendly” medical interventions aimed at engaging users to adopt recommended health behaviors are best developed in a collaborative bio-medical and social sciences setting. In the 1990s, National Breast and Colorectal Cancer Early Detection Programs were launched at the Israeli Department of Community Medicine and Epidemiology. Operating under the largest HMO (Health Maintenance Organization) in Israel (“Clalit Health Services”), the department had direct access to HMO community primary-care clinics’ teams, insured members, and medical records. Academically affiliated, the department engaged in translational research. In a decades-long translational process, this multi-disciplinary unit led a series of interventions built upon basic and applied behavioral/social science phenomena such as framing, “Implementation Intentions,” and “Question-Behavior-Effect”. A heterogeneous team of disciplinary specialists created an integrated scientific environment. In order to enhance screening, the team focused on the establishment of a systematic mechanism actively inviting programs’ “users” (average-risk targeted individuals on the national level), and continuously applied social and health psychology concepts to study individuals’ perceptions, expectations, and needs related to cancer screening. The increase in adherence to screening recommendations was slow and incremental. A decrease in late-stage breast and colorectal cancer diagnoses was observed nationally, but participation was lower than expected. This paper positions screening adherence as a unique challenge and proposes new social and network avenues to enhance future participation.
Mechanisms Underlying the Effects of Landscape Features of Urban Community Parks on Health-Related Feelings of Users
Authors: Zhang Lin , Liu Suyan , Liu Song
Published: July 2021

Urban community parks are closely related to the health of residents, and have a positive effect on residents’ perception of nature, alleviating anxiety, and promoting physical health. Many previous studies have examined the impact of community parks on the health of the population, but few studies have investigated the potential of specific landscape elements in community parks to restore physical health. We conducted psychological questionnaires with 440 users of community parks in Shanghai through on-site surveys. Based on the psychological questionnaire, a structural equation model of the relationship between the community park landscape environment and users’ feelings was established. The model indicated that the natural environment, activity environment, and rest environment in the community park had positive effects on the physical, mental, and social health of users. At the same time, we recruited 50 participants to conduct laboratory experiments examining physiological changes while participants viewed different types of scene photographs showing the same landscape element. By measuring physiological indicators, including skin conductivity and heart rate, we sought to identify the types of landscape elements that help relieve the stress of users. The results revealed that flower clusters and waterscapes in the natural environment landscape, plastic fitness trails and fitness equipment places in the sports area, landscape elements such as benches with backrests, Chinese style pavilions, and green corridors with plants in the rest space, played positive roles in alleviating feelings of pressure and promoting relaxation among community park users. Based on these findings, we propose specific design strategies to improve the landscape health of community parks.
Success Factors for Community Health Workers in Implementing an Integrated Group-Based Child Development Intervention in Rural Bangladesh
Authors: Jahir Tania , Winch Peter J. , Leontsini Elli , Hwang Sharon T. , Yeasmin Farzana , Hossain Khobair , Das Jyoti Bhushan , Amin Ruhul , Nurul Huda Tarique Md. , Sultana Jesmin , Khan Rizwana , Akter Fahmida , Shoab AKM , Hasan Rezaul , Pitchik Helen O. , Tofail Fahmida , Fernald Lia C. H. , Luby Stephen P. , Rahman Mahbubur
Published: July 2021

Community Health Workers (CHWs) can effectively implement maternal and child health interventions, but there is paucity of evidence on how to integrate child stimulation into these interventions, and their delivery at scale. In rural Bangladesh, CHWs implemented an intervention integrating psychosocial stimulation, nutrition, maternal mental health, water, sanitation, hygiene (WASH) and lead exposure prevention. In each of 16 intervention villages, one CHW worked with 20 households. CHWs bi-weekly held group meetings or alternated group meetings and home visits with pregnant women and lactating mothers. We assessed the intervention through five focus groups, four interviews and one group discussion with CHWs and their supervisors to explore success factors of implementation. CHWs’ training, one-on-one supervision and introduction by staff to their own community, and adoption of tablet computers as job aids, enabled successful session delivery to convey behavioral recommendations. CHWs reported difficulties delivering session due to the complexity of behavioral recommendations and struggled with age-specific intervention material. Young children’s attendance in group sessions generated distractions that undermined content delivery. We identified ways to minimize the difficulties to strengthen intervention-delivery during implementation, and scale-up. Iterative revisions of similarly integrated interventions based on qualitative evaluation findings could be delivered feasibly by CHWs and allow for implementation at scale.
Moderating Effects of Founders’ Role on the Influence of Internationalization on IPO Performance of Listed Companies in Thailand
Authors: Kongkaew Tharitsaya , Tongkong Supa , Ngudgratoke Sungworn
Published: July 2021

Previous research suggests that internationalization affects initial public offering (IPO) performance in the short term, but it less is known about the founders’ role in the relationship between internationalization and IPO performance. The objectives of this study were to investigate moderating effects of the founders’ role on the impact of internationalization on IPO performance of newly listed companies. The samples included 80 international firms listed in Thailand stock markets from 2013 to 2020. Multiple regression analysis was employed to test the effects of internationalization on IPO performance, and the PROCESS macro was applied to test the moderating effects. Founder CEO as a proxy of a founders’ role was a moderator variable where internationalization was a predictor variable and IPO underpricing, a proxy of IPO performance, was the outcome variable. The results revealed that internationalization demonstrated no statistically significant effect on IPO underpricing. A non-founder CEO had a moderating effect on the influence of internationalization on IPO underpricing, whereas a founder CEO revealed no moderating effect. Specifically, internationalization had a negative effect on IPO underpricing once an international firm had a non-founder CEO. A decrease in IPO underpricing of international firm is clearly explained by the results of this study.
Polymeric Microspheres/Cells/Extracellular Matrix Constructs Produced by Auto-Assembly for Bone Modular Tissue Engineering
Authors: Mielan Bartosz , Sousa Daniela , Krok-Borkowicz Małgorzata , Eloy Pierre , Dupont Christine , Lamghari Meriem , Pamuła Elżbieta
Published: July 2021

Modular tissue engineering (MTE) is a novel “bottom-up” approach to create engineered biological tissues from microscale repeating units. Our aim was to obtain microtissue constructs, based on polymer microspheres (MSs) populated with cells, which can be further assembled into larger tissue blocks and used in bone MTE. Poly(L-lactide-co-glycolide) MS of 165 ± 47 µm in diameter were produced by oil-in-water emulsification and treated with 0.1 M NaOH. To improve cell adhesion, MSs were coated with poly-L-lysine (PLL) or human recombinant collagen type I (COL). The presence of oxygenated functionalities and PLL/COL coating on MS was confirmed by X-ray photoelectron spectroscopy (XPS). To assess the influence of medium composition on adhesion, proliferation, and osteogenic differentiation, preosteoblast MC3T3-E1 cells were cultured on MS in minimal essential medium (MEM) and osteogenic differentiation medium (OSG). Moreover, to assess the potential osteoblast–osteoclast cross-talk phenomenon and the influence of signaling molecules released by osteoclasts on osteoblast cell culture, a medium obtained from osteoclast culture (OSC) was also used. To impel the cells to adhere and grow on the MS, anti-adhesive cell culture plates were utilized. The results show that MS coated with PLL and COL significantly favor the adhesion and growth of MC3T3-E1 cells on days 1 and 7, respectively, in all experimental conditions tested. On day 7, three-dimensional MS/cell/extracellular matrix constructs were created owing to auto-assembly. The cells grown in such constructs exhibited high activity of early osteogenic differentiation marker, namely, alkaline phosphatase. Superior cell growth on PLL- and COL-coated MS on day 14 was observed in the OSG medium. Interestingly, deposition of extracellular matrix and its mineralization was particularly enhanced on COL-coated MS in OSG medium on day 14. In our study, we developed a method of spontaneous formation of organoid-like MS-based cell/ECM constructs with a few millimeters in size. Such constructs may be regarded as building blocks in bone MTE.
Fibroblast Growth Factor 21 Response in a Preclinical Alcohol Model of Acute-on-Chronic Liver Injury
Authors: Christidis Grigorios , Karatayli Ersin , Hall Rabea A. , Weber Susanne N. , Reichert Matthias C. , Hohl Mathias , Qiao Sen , Boehm Ulrich , Lütjohann Dieter , Lammert Frank , Karatayli Senem Ceren
Published: July 2021

Background and Aims: Fibroblast growth factor (FGF) 21 has recently been shown to play a potential role in bile acid metabolism. We aimed to investigate the FGF21 response in an ethanol-induced acute-on-chronic liver injury (ACLI) model in Abcb4−/− mice with deficiency of the hepatobiliary phospholipid transporter. Methods: Total RNA was extracted from wild-type (WT, C57BL/6J) and Abcb4−/− (KO) mice, which were either fed a control diet (WT-Cont and KO-Cont groups; n = 28/group) or ethanol diet, followed by an acute ethanol binge (WT-EtOH and KO-EtOH groups; n = 28/group). A total of 58 human subjects were recruited into the study, including patients with alcohol-associated liver disease (AALD; n = 31) and healthy controls (n = 27). The hepatic and ileal expressions of genes involved in bile acid metabolism, plasma FGF levels, and bile acid and its precursors 7α- and 27-hydroxycholesterol (7α- and 27-OHC) concentrations were determined. Primary mouse hepatocytes were isolated for cell culture experiments. Results: Alcohol feeding significantly induced plasma FGF21 and decreased hepatic Cyp7a1 levels. Hepatic expression levels of Fibroblast growth factor receptor 1 (Fgfr1), Fgfr4, Farnesoid X-activated receptor (Fxr), and Small heterodimer partner (Shp) and plasma FGF15/FGF19 levels did not differ with alcohol challenge. Exogenous FGF21 treatment suppressed Cyp7a1 in a dose-dependent manner in vitro. AALD patients showed markedly higher FGF21 and lower 7α-OHC plasma levels while FGF19 did not differ. Conclusions: The simultaneous upregulation of FGF21 and downregulation of Cyp7a1 expressions upon chronic plus binge alcohol feeding together with the invariant plasma FGF15 and hepatic Shp and Fxr levels suggest the presence of a direct regulatory mechanism of FGF21 on bile acid homeostasis through inhibition of CYP7A1 by an FGF15-independent pathway in this ACLI model. Lay Summary: Alcohol challenge results in the upregulation of FGF21 and repression of Cyp7a1 expressions while circulating FGF15 and hepatic Shp and Fxr levels remain constant both in healthy and pre-injured livers, suggesting the presence of an alternative FGF15-independent regulatory mechanism of FGF21 on bile acid homeostasis through the inhibition of Cyp7a1.
The Effect of Ferula communis Extract in Escherichia coli Lipopolysaccharide-Induced Neuroinflammation in Cultured Neurons and Oligodendrocytes
Authors: Maiuolo Jessica , Bava Irene , Carresi Cristina , Gliozzi Micaela , Musolino Vincenzo , Scicchitano Miriam , Macri Roberta , Oppedisano Francesca , Scarano Federica , Caterina Zito Maria Caterina , Bosco Francesca , Ruga Stefano , Nucera Saverio , Ilari Sara , Palma Ernesto , Muscoli Carolina , Mollace Vincenzo
Published: July 2021

In recent decades, interest in natural compounds has increased exponentially due to their numerous beneficial properties in the treatment of various acute and chronic diseases. A group of plant derivatives with great scientific interest is terpenic compounds. Among the plants richest in terpenes, the genus Ferula L. is one of the most representative, and ferutinin, the most common sesquiterpene, is extracted from the leaves, rhizome, and roots of this plant. As reported in the scientific literature, ferutinin possesses antioxidant and anti-inflammatory properties, as well as valuable estrogenic properties. Neurodegenerative and demyelinating diseases are devastating conditions for which a definite cure has not yet been established. The mechanisms involved in these diseases are still poorly understood, and oxidative stress is considered to be both a key modulator and a common denominator. In the proposed experimental system, co-cultured human neurons (SH-SY5Y) and human oligodendrocytes (MO3.13) were treated with the pro-inflammatory agent lipopolysaccharide at a concentration of 1 μg/mL for 24 h or pretreated with ferutinin (33 nM) for 24 h and subsequently exposed to lipopolysaccharide 1 μg/mL for 24 h. Further studies would, however, be needed to establish whether this natural compound can be used as a support strategy in pathologies characterized by progressive inflammation and oxidative stress phenomena.