bluuebunny/crossref_metadata_2025 huggingface dataset:
Test of the Equality of Several High-Dimensional Covariance Matrices: A Normal-Reference Approach
Authors: Wang Jingyi , Zhu Tianming , Zhang Jin-Ting
Published: January 2025

As the field of big data continues to evolve, there is an increasing necessity to evaluate the equality of multiple high-dimensional covariance matrices. Many existing methods rely on approximations to the null distribution of the test statistic or its extreme-value distributions under stringent conditions, leading to outcomes that are either overly permissive or excessively cautious. Consequently, these methods often lack robustness when applied to real-world data, as verifying the required assumptions can be arduous. In response to these challenges, we introduce a novel test statistic utilizing the normal-reference approach. We demonstrate that the null distribution of this test statistic shares the same limiting distribution as a chi-square-type mixture under certain regularity conditions, with the latter reliably estimable from data using the three-cumulant matched chi-square-approximation. Additionally, we establish the asymptotic power of our proposed test. Through comprehensive simulation studies and real data analysis, our proposed test demonstrates superior performance in terms of size control compared to several competing methods.
Coarse-Grained Column Agglomeration Parallel Algorithm for LU Factorization Using Multi-Threaded MATLAB
Authors: Sabir Osama , Alebrahim Reza
Published: January 2025

MATLAB programing language is one of the most popular scientific computing tools, especially for solving linear algebra problems. LU factorization is an essential component for the direct solution of linear equations systems. This paper studied a coarse-grained column agglomeration parallel algorithm in MATLAB to analyze the implementation performance among all the available computation resources. In this paper, we focus on parallelizing the LU decomposition without pivoting algorithm using Gaussian elimination under MATLAB R2020b platform. Numerical experiments were provided to demonstrate the efficiency of CPU parallelization. Performances of the present methods were assessed by comparing the speed and accuracy of different coarse-grained column agglomeration algorithms using different sizes of matrices. Different algorithms were implemented in a four-core Xeon E3-1220 v3 @ 3.10 GHz CPU with 16 GB RAM memory.
Key Taxa of the Gut Microbiome Associated with the Relationship Between Environmental Sensitivity and Inflammation-Related Biomarkers
Authors: Takasugi Satoshi , Iimura Shuhei , Yasuda Miyabi , Saito Yoshie , Morifuji Masashi
Published: January 2025

Individual differences in environmental sensitivity are linked to stress-related psychiatric symptoms. In previous research, we found that high environmental sensitivity can be a risk factor for increased inflammation and gut permeability, particularly when gut microbiome diversity is low. However, the specific gut bacterial taxa involved in this interaction remain unclear. As a preliminary study, this research aimed to identify the key gut microbiome taxa associated with this relationship. Environmental sensitivity, gut microbiome composition, gut permeability (lipopolysaccharide-binding protein, LBP), and inflammation (C-reactive protein, CRP) biomarkers were evaluated in 88 participants. The interaction between environmental sensitivity and the relative abundance of the family Marinifilaceae (genus Butyricimonas) was a predictor of CRP levels. Similarly, the interaction between environmental sensitivity and relative abundance of the family Barnesiellaceae (genus Coprobacter), the family Akkermansiaceae (genus Akkermansia), the genus Family XIII AD3011 group, the genus GCA-900066225, or the genus Ruminiclostridium 1 predicted LBP levels. Individuals with high environmental sensitivity exhibited elevated CRP or LBP levels when the relative abundance of these taxa was low. Conversely, highly sensitive individuals had lower CRP or LBP levels when the relative abundance of these taxa was high. This study suggests that specific taxa serve as one of the protective factors against inflammation and gut permeability in individuals with high environmental sensitivity. Further in-depth studies are needed to confirm these associations and understand the underlying mechanisms.
Four Decades of Bacillus Biofertilizers: Advances and Future Prospects in Agriculture
Authors: Wu Xinmai , Liu Yan , Jia Baolei , Tao Lili , Li Han , Wang Jingbang , Yuan Ziqi , Sun Xiaobao , Yao Yanlai
Published: January 2025

Over the past four decades, Bacillus biofertilizers, which are microbial formulations based on Bacillus species, have significantly contributed to sustainable agriculture by enhancing crop growth, improving soil health, and reducing the dependency on chemical fertilizers. Bacillus species, particularly known for their ability to promote plant growth, fix nitrogen, solubilize phosphorus, and produce growth-promoting substances such as phytohormones and antibiotics, have emerged as key players in the development of eco-friendly agricultural solutions. This research utilizes bibliometric analysis based on 3,242 documents sourced from the Web of Science database to map the development, key contributions, and innovation within the field from 1985 to 2023. This study identifies exponential growth in research output, particularly from 2003 onwards, indicating a robust interest and expanding research base predominantly in China, India, and the United States. We segmented the research timeline into three distinct phases, each marked by varying growth rates and research foci. This paper presents novel insights into the geographical and institutional distributions of research, highlighting the predominant role of developing countries in advancing Bacillus-based technologies. Key research hotspots have evolved from basic applications to complex interactions involving synthetic microbial communities and advanced multi-omics techniques. Our findings demonstrate a trend towards more strategic and technologically integrated approaches to developing Bacillus biofertilizers, reflecting broader shifts towards more sustainable agricultural systems. This study not only charts historical progress, but also proposes future research trajectories aimed at enhancing the application and effectiveness of microbial fertilizers across diverse ecosystems.
First Detection and Genomic Characterization of Linezolid-Resistant Enterococcus faecalis Clinical Isolates in Bulgaria
Authors: Strateva Tanya V. , Hristova Preslava , Stoeva Temenuga J. , Hitkova Hristina , Peykov Slavil
Published: January 2025

Linezolid is an oxazolidinone antibiotic and is considered a last-resort treatment option for serious infections caused by problematic Gram-positive pathogens, including vancomycin-resistant enterococci. The present study aimed to explore the linezolid resistance mechanisms and genomic characteristics of two vancomycin-susceptible Enterococcus faecalis isolates from Bulgaria. The strains designated Efs2503-bg (inpatient from Pleven) and Efs966-bg (outpatient from Varna) were recovered from wounds in 2018 and 2023, respectively. Antimicrobial susceptibility testing, whole-genome sequencing, multilocus sequence typing, and phylogenomic analysis based on 332 linezolid-resistant E. faecalis genomes were performed. Efs2503-bg was high-level resistant to linezolid (MIC > 256 mg/L) and displayed the G2576T mutation affecting three of the four 23S rDNA loci. Efs966-bg (MIC = 8 mg/L) carried a plasmid-located optrA determinant surrounded by fexA and ermA. No mutations in the genes encoding for ribosomal proteins L3, L4, and L22 were detected. The isolates belonged to the sequence types ST6 (Efs2503-bg) and ST1102 (Efs966-bg). Phylogenomic analysis revealed that Efs2503-bg and Efs966-bg are genetically distinct, with a difference of 12,051 single-nucleotide polymorphisms. To our knowledge, this is the first report of linezolid-resistant enterococci in Bulgaria. Although the global incidence of linezolid-resistant enterococci is still low, their emergence is alarming and poses a growing clinical threat to public health.
The Role of Synthesis Methods of Ceria-Based Catalysts in Soot Combustion
Authors: Grzybek Gabriela , Wójtowicz Andrzej , Legutko Piotr , Greluk Magdalena , Słowik Grzegorz , Sienkiewicz Andrzej , Adamski Andrzej , Kotarba Andrzej
Published: January 2025

The removal of soot particles via high-performance catalysts is a critical area of research due to the growing concern regarding air pollution. Among various potential catalysts suitable for soot oxidation, cerium oxide-based materials have shown considerable promise. In this study, CeO2 samples obtained using a range of preparation methods (including hydrothermal synthesis (HT), sonochemical synthesis (SC), and hard template synthesis (TS)) were tested in soot combustion. They were compared to commercially available material (COM). All synthesized ceria catalysts were thoroughly characterized using XRD, RS, UV/Vis-DR, XPS, H2-TPR, SEM, and TEM techniques. As confirmed in the current study, every tested ceria sample can be used as an effective soot oxidation catalyst, with a temperature of 50% soot conversion not exceeding 400 °C in a tight contact mode. A strong correlation was observed between the catalysts’ Ce3+ concentration and activity, with higher Ce3+ levels leading to improved performance. These findings underscore the importance of synthesis in optimizing ceria-based catalysts for environmental applications.
Phenolic Acid and Flavonoid Content Analysis with Antioxidant Activity Assessment in Chinese C. pi. Shen Honey
Authors: Qi Ningxin , Zhao Wen , Xue Chenghua , Zhang Lin , Hu Han , Jin Yue , Xue Xiaofeng , Chen Rui , Zhang Jinzhen
Published: January 2025

The nutritional value of honey is closely related to its components, which are highly influenced by the botanic origin. C. pi. Shen (Codonopsis pilosula (Franch.) var. modesta (Nannf.) L. T. Shen), a key plant in traditional Chinese medicine, mainly produced in Gansu Province of China, has attracted much attention for its medicinal value. However, there are few reports about C. pi. Shen honey. Therefore, this study aimed to evaluate the contents of phenolic profiles and antioxidant activities in C. pi. Shen honey by colorimetric, UPLC-MS/MS, Ferric ion Reducing Antioxidant Power (FRAP), 2,2-bisazo-bis (3-ethyl-benzothiazole-6-sulfonic acid) (ABTS) free radical capacity, and 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) scavenging ability methods. In comparison with four other high-yield unifloral honeys in China (Acacia honey, linden honey, rape honey, and jujube honey), C. pi. Shen honey demonstrated remarkable advantages. Specifically, the levels of total phenolic acids, total flavonoids, and phenolic components of C. pi. Shen honey were significantly pronounced, especially protocatechuic acid and kaempferol. Furthermore, the antioxidant activity of C. pi. Shen honey was also excellent, which was attributed to its phenolic compounds, underscoring its potential biological value. This study is anticipated to serve as a reference for the identification, nutritional assessment, and functional advancement of C. pi. Shen honey.
Diastereodivergent Construction of Octahydrophenanthridinone and Octahydrophenanthridine Cores
Authors: Sun Chunzhao , Nishikawa Hiromichi , Inokuma Tsubasa , Yamada Ken-ichi
Published: January 2025

Diastereodivergent synthesis of octahydrophenanthridinone and octahydrophenanthridine skeletons, structural motifs often found in biologically active natural products, is described. We previously reported a total synthesis of a pancratistatin analog using novel octahydrophenanthridinone construction. In this study, we examined the generality of our method and its extension to octahydrophenanthridine formation. Conjugate addition of diarylcuprates to nitrosocyclohexenes, which were generated in situ from 2-chlorocyclohexanone oximes, provided 2-arylcyclohexanone oximes. Subsequent reduction of the oxime moiety gave cis- and trans-configured amines. Both amines were separately converted into the corresponding octahydrophenanthridinones and octahydrophenanthridines via hexahydrophenanthridine intermediates.
Electrochemical Method for the Assay of Organic Peroxides Directly in Acetonitrile
Authors: Ivanova Vanina , Pimpilova Mariya , Stoyanova Maria , Dimcheva Nina
Published: January 2025

Lipid peroxidation is a major process that determines the quality of various oil samples during their use and storage, in which the primary products are hydroperoxides (HP’S). HP’S are very stable compounds at ambient conditions and are harmful to human health. Therefore, the evaluation of the degree of oil oxidation is an excellent tool for ensuring food safety. The peroxide value (PV) is the main parameter used for quality control in oils. Herein, we propose an alternative electrochemical method to the classical iodometric titration method most widely used for determining the PV. Our approach is based on the electrochemical quantification of hydroperoxides/peroxides in an organic solvent medium (acetonitrile and organic ammonium salt) using a composite electrocatalyst–glassy carbon electrode modified with 2D-nanomaterial graphitic carbon nitride doped with Co3O4. Calibration was made by the method of standard addition using benzoyl peroxide (BPO) as a model peroxide compound, dissolved in chloroform and added to fresh Rivana-branded anti-cellulite oil, used as a model oil sample. Calibration plots showed a linear response and the very good reproducibility of the analytical result (R2 ˃ 0.99). Further, in terms of accuracy, the method showed good results, since the BPO quantitative analysis was close to the theoretical response. In addition, the accuracy of the electrochemical method was compared with that of the standard iodometric titration method for determining the PV of vegetable fats (according to a standard method). Finally, using the electrochemical method, the concentration of peroxides was determined in a real sample—an anti-cellulite oil of the trademark Rivana with an expired shelf life.
Mechanistic Pathways in Cyanide-Mediated Benzoin Condensation: A Comprehensive Electron Localisation Function (ELF) and Catastrophe Theory Analysis of the Umpolung Reaction
Authors: Michalski Michal , Berski Slawomir
Published: January 2025

This research investigates the mechanism of the cyanide-type umpolung reaction in benzoin condensation using topological analysis of ELF and catastrophe theory. The study achieves a comprehensive understanding of the evolution of chemical bonds and non-bonding electron density in the reaction of benzaldehyde and cyanide ions. The results reveal that the reaction proceeds through five transition state structures, with the formation of Lapworth’s cyanohydrin being the rate-determining step. The study characterises topological catastrophes in the evolution of the ELF field and provides a detailed description of the evolution of electron density in the mechanism of the reaction. An in-depth analysis of ELF catastrophes confirms the well-established Lapworth mechanism.
Self-Assembly Strategy for Synthesis of WO3@TCN Heterojunction: Efficient for Photocatalytic Degradation and Hydrogen Production via Water Splitting
Authors: Zhou Li , Zhang Wenjie , Huang Zezhao , Hu Feng , Li Peng , Yao Xiaoquan
Published: January 2025

Herein, a WO3@TCN photocatalyst was successfully synthesized using a self-assembly method, which demonstrated effectiveness in degrading organic dyestuffs and photocatalytic evolution of H2. The synergistic effect between WO3 and TCN, along with the porous structure of TCN, facilitated the formation of a heterojunction that promoted the absorption of visible light, accelerated the interfacial charge transfer, and inhibited the recombination of photogenerated electron–hole pairs. This led to excellent photocatalytic performance of 3%WO3@TCN in degrading TC and catalyzing H2 evolution from water splitting under visible-light irradiation. After modulation, the optimal 3%WO3@TCN exhibited a maximal degradation rate constant that was twofold higher than that of TCN alone and showed continuous H2 generation in the photocatalytic hydrogen evolution. Mechanistic studies revealed that •O2− constituted the major active species for the photocatalytic degradation of tetracycline. Experimental and DFT results verified the electronic transmission direction of WO3@TCN heterojunction. Overall, this study facilitates the structural design of green TCN-based heterojunction photocatalysts and expands the application of TCN in the diverse photocatalytic processes. Additionally, this study offers valuable insights into strategically employing acid regulation modulation to enhance the performance of carbon nitride-based photocatalysts by altering the topography of WO3@TCN composite material dramatically.
The Counteracting Effect of Chrysin on Dietary Fructose-Induced Metabolic-Associated Fatty Liver Disease (MAFLD) in Rats with a Focus on Glucose and Lipid Metabolism
Authors: Campanher Gabriela , Andrade Nelson , Lopes Joanne , Silva Cláudia , Pena Maria João , Rodrigues Ilda , Martel Fátima
Published: January 2025

The prevalence of metabolic syndrome has been exponentially increasing in recent decades. Thus, there is an increasing need for affordable and natural interventions for this disorder. We explored the effect of chrysin, a dietary polyphenol, on hepatic lipid and glycogen accumulation, metabolic dysfunction-associated fatty liver disease (MAFLD) activity score and oxidative stress and on hepatic and adipose tissue metabolism in rats presenting metabolic syndrome-associated conditions. Rats fed a chow diet were separated into four groups: Control (tap water), Fructose (tap water with 10% fructose), Chrysin (tap water+ chrysin (100 mg/kg body weight/d)), and Fructose + Chrysin (tap water with 10% fructose + chrysin (100 mg/kg body weight/d, daily)) (for 18 weeks). When associated with the chow diet, chrysin reduced hepatic lipid and glycogen storage, increased the hepatic antioxidant potential of glutathione and reduced de novo lipogenesis in the adipose tissue. When associated with the high fructose-diet, chrysin attenuated the increase in lipid and glycogen hepatic storage, improved the MAFLD activity score, decreased hepatic lipid peroxidation, increased the antioxidant potential of glutathione, and improved lipid and glucose metabolic markers in the liver and adipose tissue. In conclusion, our results suggest that chrysin is a beneficial addition to a daily diet for improvement of hepatic metabolic health, particularly for individuals suffering from metabolic syndrome.
Differences in Nanoplastic Formation Behavior Between High-Density Polyethylene and Low-Density Polyethylene
Authors: Nakatani Hisayuki , Yamaguchi Teruyuki , Asano Mika , Motokucho Suguru , Dao Anh Thi Ngoc , Kim Hee-Jin , Yagi Mitsuharu , Kyozuka Yusaku
Published: January 2025

High-Density Polyethylene (HDPE) and Low-Density Polyethylene (LDPE) films were used to create nanoplastic (NP) models, with the shape of delamination occurring during degradation. In the case of HDPE, selective degradation occurred not only in the amorphous part, but also in the crystalline part at the same time. Some of the lamellae that extend radially to form the spherulite structure were missing during the 30-day degradation. The length of these defects was less than 1 µm. HDPE disintegrated within units of spherulite structure by conformational defects in lamellae, and the size of the fragments obtained had a wide distribution. LDPE was synthesized by radical polymerization, so it contained a cross-linked part. The part was not sufficiently fused, and when it degraded, it delaminated and separated preferentially. The zeta potential reached a minimum value of approximately −20 mV at the degradation time of 21 days, and then increased. This complex dependence on degradation time was due to NP particle aggregation. The addition of 1% Triton(R) X-114 surfactant was effective in stabilizing the NP dispersion. The particle size remained constant at around 20 nm for degradation times of 15–30 days.
Trifolirhizin: A Phytochemical with Multiple Pharmacological Properties
Authors: Jaiswal Varun , Lee Hae-Jeung
Published: January 2025

Trifolirhizin is an important flavonoid glycoside reported from the roots of medicinal plants such as Astragalus membranaceus, Sophora tonkinensis, Ononis vaginalis, Euchresta formosana, Sophora Subprostrate, Ononis spinose, and Sophora flavescens. It is considered one of the important constituents responsible for the various medicinal properties of these medicinal plants. Studies have revealed the multiple pharmacological properties of trifolirhizin: anti-inflammatory, antioxidant, antibacterial, anti-ulcerative colitis, antiasthma, hepatoprotective, osteogenic, skin-whitening, wound-healing, and anticancer (against various types of cancers). Mechanistic studies of trifolirhizin showed that it could act on important target genes and pathways such as the NF-κB-MAPK, EGFR-MAPK, AMPK/mTOR, and PI3K/Akt signaling pathways. These pathways are also implicated in various other diseases, suggesting the potential of trifolirhizin in therapeutic applications. Initial pharmacokinetic studies support the therapeutic candidature of trifolirhizin and provide the initial track that may be pursued for its development. Still, a compilation of pharmacological activities and target pathways of trifolirhizin is missing in the literature. This review uniquely compiles the pharmacological properties and mechanistic insights of trifolirhizin, addressing critical gaps in its therapeutic development and proposing strategies for future research.
6-Bromoindole- and 6-Bromoindazole-Based Inhibitors of Bacterial Cystathionine γ-Lyase Containing 3-Aminothiophene-2-Carboxylate Moiety
Authors: Novikov Roman A. , Platonov Dmitry N. , Belyy Alexander Yu. , Potapov Konstantin V. , Novikov Maxim A. , Tomilov Yury V. , Kechko Olga I. , Seregina Tatiana A. , Zemskaya Anastasia S. , Solyev Pavel N. , Mitkevich Vladimir A.
Published: January 2025

In recent years, a number of synthetic potentiators of antibiotics have been discovered. Their action can significantly enhance the antibacterial effect and limit the spread of antibiotic resistance through inhibition of bacterial cystathionine-γ-lyase. To expand the known set of potentiators, we developed methods for the synthesis of five new representatives of 6-bromoindole derivatives—potential inhibitors of bacterial cystathionine-γ-lyase—namely potassium 3-amino-5-((6-bromoindolyl)methyl)thiophene-2-carboxylate (MNS2) and its 6-bromoindazole analogs (MNS3 and MNS4), along with two 6-broindazole analogs of the parent compound NL2. Their syntheses are based on 6-bromoindole, 6-bromoindazole and methyl 5-(bromomethyl)-3-((ethoxycarbonyl)amino)thiophene-2-carboxylate as the main building blocks, assembling the rest of the heterocyclic system on their basis at the nitrogen atom. We assessed the ability of the new inhibitors to potentiate the antimicrobial activity of gentamicin.
The Relationship Between a Mediterranean Diet and Frailty in Older Adults: NHANES 2007–2017
Authors: Gross Danae C. , Dahringer Jessica C. , Bramblett Paige , Sun Chang , Spangler Hillary B. , Lynch David H. , Batsis John A.
Published: January 2025

Background: Frailty is a geriatric syndrome of significant public health concern that causes vulnerability to physiologic stressors and an increased risk of mortality and hospitalizations. Dietary intake and quality are contributing factors to the development of frailty. The Mediterranean diet is known to be one of the healthiest eating patterns with promising health impacts for prevention. We evaluated the association between Mediterranean diet patterns and frailty status. Methods: We conducted a cross-sectional study using National Health and Nutrition Examination Survey data from 2007 to 2017. We included 7300 participants aged > 60 years who completed the first day of a 24 h diet recall and had full covariate data. We constructed an alternate Mediterranean diet (aMED) score based on the quantity of specific food-group intake and categorized participants to low-, moderate-, and high-adherence groups (aMED adherence scores of 0–2, 3–4, and 5–9, respectively). Using a modified Fried Frailty phenotype (weakness, low physical activity, exhaustion, slow walking speed, and weight loss), participants were categorized as robust (met no criteria), pre-frail (met one or two criteria), and frail (met three or more criteria). Logistic regression evaluated the association of frailty (prefrail/robust as referent) and aMED adherence. Results: Included participants were mainly female (54.5%) and non-Hispanic White (80.0%). The mean (SD) aMED score was 3.6 (1.6) with 45% of participants falling into moderate aMED adherence (26% low adherence, 30% high adherence). Frailty prevalence among participants was 7.1%, with most participants classified as robust (51.0%) or pre-frail (41.9%). Fully adjusted models showed significantly reduced odds of frailty with moderate-adherence and high-adherence groups (odds ratio (95%CI) of 0.71 (0.55, 0.92) and 0.52 (0.36, 0.75), respectively). Conclusions: Mediterranean diet adherence is associated with decreased odds of frailty in older adults. These findings suggest that adherence to a Mediterranean diet may play a critical role in mitigating frailty and its associated conditions. Future research should include longitudinal and interventional studies that can definitively determine the effect of a Mediterranean diet on frailty and what food components provide the greatest benefit.
Very-Low-Absorbable Geraniol for the Treatment of Irritable Bowel Syndrome: A “Real-World” Open-Label Study on 1585 Patients
Authors: Ricci Chiara , Saracino Ilaria Maria , Valerii Maria Chiara , Spigarelli Renato , Bellocchio Irene , Spisni Enzo
Published: January 2025

Objective: The objective of this study was to evaluate the efficacy of a very-low-absorbable geraniol formulation, administered as a food supplement, in patients with irritable bowel syndrome (IBS) in a real-world setting in Italy. Methods: This open-label study was conducted in Italy on patients diagnosed with IBS and treated for 4 weeks with 240 mg/day of Palmarosa essential oil, absorbed on 960 mg of ginger root powder to obtain a very-low-absorbable geraniol formulation. Baseline characteristics, including demographic and symptoms were recorded using the IBS Severity Scoring System (IBS-SSS). After 28 ± 7 days, the patients were asked to complete the IBS-SSS questionnaire again. The primary objective was to confirm the effects of a very-low-absorbable geraniol formulation on self-reported symptoms of IBS and the quality of life of affected individuals. The secondary objective was to confirm the effect of the treatment on the different IBS subtypes. Results: A total of 1585 patients were included in the study, with a mean age of 44.8 years and 56.4% women. Following the 4-week supplementation period, significant decreases were observed in the patients’ IBS-SSS (−67.9%) and all the primary IBS symptoms, such as abdominal distention (−82.3%), unsatisfaction with bowel habits (−46.2%), and interference with quality of life (QoL) (−64.9%) (all p < 0.01). The patients’ stool type improved significantly. Treatment was effective in all IBS subtypes. Conclusions: Treatment with very-low-adsorbable geraniol food supplement was associated with improvements in symptoms and bowel habits in all IBS subtypes in a real-world setting in Italy. These findings support the use of geraniol as an effective option for patients with IBS regardless of the disease subtype.
TAS1R2 rs35874116 Associations with Taste, Diet, and Health in an Italian Population
Authors: Stevens Harry , Graham Catherine Anna-Marie , Concas Maria Pina , Piluso Francesco , Mavrommatis Yiannis , King Alexandra , Pilic Leta , Gasparini Paolo
Published: January 2025

Background/Objectives: The TAS1R2 SNP rs35874116 has previously been associated with sweet taste, diet, and health status, although never comprehensively in a single study. Also, associations between TAS1R2 and sweet taste might be body mass index (BMI)-dependent. Therefore, this study aimed to conduct a comprehensive investigation of rs35874116 and sweet taste intensity and liking, food liking, and diet and health status whilst considering BMI. Methods: Five-hundred and fifty-four participants were recruited. Linear regression models were used to explore rs35874116 associations with sweet taste intensity and liking, food liking, and diet and health status. A secondary analysis stratified participants by BMI <25/≥25 kg/m2. Results: The rs35874116 wildtype was associated with increased sweet taste intensity (p = 0.0345, B 1.29, SE 0.61) and liking (p = 0.021, B 0.25, SE 0.11). However, these associations only remained in BMI ≥25 individuals (intensity: p = 0.037, B 1.29, SE 0.61, liking: p = 0.008, B 0.46, SE 0.17). It was also associated with decreased diet quality (p = 0.03, B −0.27, SE 0.13) and reduced free sugar consumption but increased saturated fat consumption in BMI ≥25 individuals (free sugars: p = 0.0416, B −0.8, SE 0.38, saturated fat: p = 0.031, B 1.38, SE 0.62). There was no association with the mean liking score for sweet foods, although there were some associations with individual foods, which require further investigation. There were no associations with health status. Conclusions: This study revealed an association between the rs35874116 wildtype and an increased intensity and liking of the sweet taste and a corresponding worse overall dietary quality. This study adds to previous evidence regarding how associations between TAS1R2 and sweet taste are BMI-dependent.
An All-Fiber Curvature Sensor with High Sensitivity Based on Sphere-Shaped Misaligned Structure
Authors: Li Xiaowei , Chen Qiangshen , Ren Mengyu , Feng Guoying
Published: January 2025

In this paper, a high-linear-sensitivity fiber curvature sensor based on the sphere-shaped misaligned structure (SSMS) with few-mode fiber (FMF) and single-mode fiber (SMF) was proposed and demonstrated. A spherical structure was prepared at one end of a few-mode fiber, which could effectively excite higher-order modes and generate interference in the misaligned cascade. When external environmental parameters changed, the resonance peaks formed by intermodal interference were displaced, and the shifts generated by different resonant peaks were also different. The experimental results show that the maximum curvature sensitivity was −2.220 nm/m−1, and the linear fitting coefficient reached up to 0.991, which is an extremely high sensitivity among wavelength-modulated curvature sensors. Meanwhile, the strain sensitivity of the sensor was as low as 7.99 pm/με¯, and the temperature sensitivity was 3.958 pm/°C, which is a low temperature sensitivity and low strain sensitivity, and solves the cross-sensitivity problem. With advantages of simple manufacture, low cost, and favorable stability, the sensor is expected to be one of the best candidate instruments for measuring curvature and inclination.
Neuroprotective Actions of Cannabinoids in the Bovine Isolated Retina: Role of Hydrogen Sulfide
Authors: Bush Leah , Okolie Anthonia , Robinson Jenaye , Muili Fatima , Opere Catherine A. , Ohia Sunny E. , Njie Mbye Ya Fatou
Published: January 2025

Both hydrogen sulfide and endocannabinoids can protect the neural retina from toxic insults under in vitro and in vivo conditions. Purpose: The aim of the present study was two-fold: (a) to examine the neuroprotective action of cannabinoids [methanandamide and 2-arachidonyl glycerol (2-AG)] against hydrogen peroxide (H2O2)-induced oxidative damage in the isolated bovine retina and (b) to evaluate the role of endogenously biosynthesized hydrogen sulfide (H2S) in the inhibitory actions of cannabinoids on the oxidative stress in the bovine retina. Methods: Isolated neural retinas from cows were exposed to oxidative damage using H2O2 (100 µM) for 10 min. When used, tissues were pretreated with methanandamide (1 nM–100 nM) and 2-AG (1–10 µM) for 30 min before a 10 min treatment with H2O2 (100 µM). In some experiments, retinas were pretreated with inhibitors of the biosynthesis of H2S [cystathionine β-synthase/cystathionine γ-lyase (CBS/CSE), aminooxyacetic acid, AOAA 30 µM, or 3-mercaptopyruvate sulfurtransferase (3MST), α-keto-butyric acid, KBA 1 mM] and the CB1-receptor antagonist, AM251 (100 nM) for 30 min before treatment with methanandamide (1 nM–100 µM). Enzyme immunoassay measurement of 8-epi PGF2α (8-isoprostane) levels was performed to assess lipid peroxidation in retinal tissues. Results: In the presence of H2O2 (100 µM), methanandamide (1 nM–100 µM) and 2-AG (1–10 µM) significantly (p < 0.001) blocked the H2O2-induced elevation in 8-isoprostane levels in the isolated bovine retina. In the presence of the CB1 antagonist AM251 (100 nM), the effect of methanandamide (1 nM) on the H2O2-induced 8-isoprostane production was significantly (p < 0.001) attenuated. While AOAA (30 µM) had no significant (p > 0.05) effect on the inhibition of H2O2-induced oxidative stress elicited by methanandamide, KBA (1 mM) reversed the neuroprotective action of methanandamide. Conclusions: The cannabinoids, methanandamide and 2-AG can prevent H2O2-induced oxidative stress in the isolated bovine retina. The neuroprotective actions of cannabinoids are partially dependent upon the activation of the CB1 receptors and endogenous production of H2S via the 3-MST/CAT pathway.
(E)-1-(3-(3-Hydroxy-4-Methoxyphenyl)-1-(3,4,5-Trimethoxyphenyl)allyl)-1H-1,2,4-Triazole and Related Compounds: Their Synthesis and Biological Evaluation as Novel Antimitotic Agents Targeting Breast Cancer
Authors: Ana Gloria , Malebari Azizah M. , Noorani Sara , Fayne Darren , O’Boyle Niamh M. , Zisterer Daniela M. , Pimentel Elisangela Flavia , Endringer Denise Coutinho , Meegan Mary J.
Published: January 2025

Background/Objectives: The synthesis of (E)-1-(1,3-diphenylallyl)-1H-1,2,4-triazoles and related compounds as anti-mitotic agents with activity in breast cancer was investigated. These compounds were designed as hybrids of the microtubule-targeting chalcones, indanones, and the aromatase inhibitor letrozole. Methods: A panel of 29 compounds was synthesized and examined by a preliminary screening in estrogen receptor (ER) and progesterone receptor (PR)-positive MCF-7 breast cancer cells together with cell cycle analysis and tubulin polymerization inhibition. Results: (E)-5-(3-(1H-1,2,4-triazol-1-yl)-3-(3,4,5-trimethoxyphenyl)prop-1-en-1-yl)-2-methoxyphenol 22b was identified as a potent antiproliferative compound with an IC50 value of 0.39 mM in MCF-7 breast cancer cells, 0.77 mM in triple-negative MDA-MB-231 breast cancer cells, and 0.37 mM in leukemia HL-60 cells. In addition, compound 22b demonstrated potent activity in the sub-micromolar range against the NCI 60 cancer cell line panel including prostate, melanoma, colon, leukemia, and non-small cell lung cancers. G2/M phase cell cycle arrest and the induction of apoptosis in MCF-7 cells together with inhibition of tubulin polymerization were demonstrated. Immunofluorescence studies confirmed that compound 22b targeted tubulin in MCF-7 cells, while computational docking studies predicted binding conformations for 22b in the colchicine binding site of tubulin. Compound 22b also selectively inhibited aromatase. Conclusions: Based on the results obtained, these novel compounds are suitable candidates for further investigation as antiproliferative microtubule-targeting agents for breast cancer.
2-Aminothiophene Derivatives—New Drug Candidates Against Leishmaniasis: Drug Design, Synthesis, Pharmacomodulation, and Antileishmanial Activity
Authors: de Araújo Rodrigo Santos Aquino , Bernardo Vitória Gaspar , Tibúrcio Robert da Silva , Bedor Danilo Cesar Galindo , Campos Michel Leandro de , Pontarolo Roberto , de Sousa Julyanne Maria Saraiva , Rodrigues Klinger Antonio da Franca , Scotti Marcus Tullius , Nayarisseri Anuraj , Marchand Pascal , Mendonça-Junior Francisco Jaime Bezerra
Published: January 2025

Background/Objectives: Leishmaniasis is one of the 20 Neglected Tropical Diseases according to the WHO, affecting approximately 12 million people in four continents, generating serious public health problems. The lack of therapeutic options, associated with toxicity and the emergence of resistance to the few available drugs, makes it urgent to develop new drug options. In this context, the aims of this work are to expand the knowledge about the pharmacophore group responsible for the antileishmanial potential of 2-aminothiophene derivatives. Thus, new compounds were synthesized containing chemical modifications at the C-3, C-4, and C-5 positions of the 2-aminothiophene ring, in addition to the S-Se bioisosterism. Methods: Dozens of 2-AT and 2-aminoselenophen (2-AS) derivatives were sequentially synthesized through applications of the Gewald reaction and were then evaluated in vitro for their activities against L. amazonensis and for cytotoxicity against macrophages. Results: Several series of compounds were synthesized, and it was possible to identify some substitution patterns favorable to the activity generating compounds with IC50 values below 10 µM, such as the non-essentiality of the presence of a carbonitrile group at C-3; the importance of the presence and size of cycloalkyl/piperidinyl chains at C-4 and C-5 in modulating the activity; and the increase in activity without affecting the safety of the S/Se bioisosteric substitution. Conclusions: Taken together, these findings reaffirm the great potential of 2-aminothiophenes to generate antileishmanial drug candidates and offers contributions to the drug design of compounds with an even more promising profile for the problem of leishmaniasis.
What Is the Optimal Geometry of Dissolving Microneedle Arrays? A Literature Review
Authors: Visscher Maira , Frijlink Henderik W. , Hinrichs Wouter L. J.
Published: January 2025

The application of dissolving microneedle arrays (DMNAs) is an emerging trend in drug and vaccine delivery as an alternative for hypodermic needles or other less convenient drug administration methods. The major benefits include, amongst others, that no trained healthcare personnel is required and that the recipient experiences hardly any pain during administration. However, for a successful drug or vaccine delivery from the DMNA, the microneedles should be inserted intact into the skin. A successful penetration into the upper skin layers may be challenging because of the elastic nature of the skin; therefore, a minimum insertion force is required to overcome the total resistance force of the skin. In addition, the microneedles need to stay intact, which requires a certain mechanical strength, and be able to resist the required insertion force. In addition to the type of material with which the DMNAs are produced, the geometry of the DMNAs will also have a profound effect, not only on the mechanical strength but also on the number of insertions and penetration depth into the skin. In this review, the effects of shape, aspect ratio, length, width of the base, tip diameter and angle, and spacing of DMNAs on the aforementioned effect parameters were evaluated to answer the following question: ‘What is the optimal geometry of dissolving microneedle arrays?’.
Correction: Razzaq et al. Development of Cephradine-Loaded Gelatin/Polyvinyl Alcohol Electrospun Nanofibers for Effective Diabetic Wound Healing: In-Vitro and In-Vivo Assessments. Pharmaceutics 2021, 13, 349
Authors: Razzaq Anam , Khan Zaheer Ullah , Saeed Aasim , Shah Kiramat Ali , Khan Naveed Ullah , Menaa Bouzid , Iqbal Haroon , Menaa Farid
Published: January 2025

In the original publication [...]
Aesthetic Habits in Performing Arts
Authors: Bertinetto Alessandro
Published: January 2025

This article explores the connection between habits and the performing arts, arguing that habits are not only fundamental to the practice and appreciation of these arts but also inherently performative in nature. Drawing on insights from various philosophical traditions (including cognitive science, pragmatism, and phenomenology), it examines how habits function within artistic processes as resources for creativity and adaptation. Engaging critically with Noë’s interpretation of the entanglement between art and life, this article highlights the dual nature of habits: as routine practices that scaffold artistic expression and as dynamic, transformative elements responsive to specific cultural and performative contexts. By focusing on key notions such as gesture, style, and rituality in the performing arts, this article discusses the role of habits in aesthetic experiences, highlighting how habits shape both artistic performances and audience engagement. This perspective challenges traditional views that oppose habits to creativity, defending instead the idea that habits are creatively operative in both the performing arts and their reception.
Genome-Wide Association Study on Imputed Genotypes of 180 Eurasian Soybean Glycine max Varieties for Oil and Protein Contents in Seeds
Authors: Potapova Nadezhda A. , Zorkoltseva Irina V. , Zlobin Alexander S. , Shcherban Andrey B. , Fedyaeva Anna V. , Salina Elena A. , Svishcheva Gulnara R. , Aksenovich Tatiana I. , Tsepilov Yakov A.
Published: January 2025

Soybean (Glycine max) is a leguminous plant with a broad range of applications, particularly in agriculture and food production, where its seed composition—especially oil and protein content—is highly valued. Improving these traits is a primary focus of soybean breeding programs. In this study, we conducted a genome-wide association study (GWAS) to identify genetic loci linked to oil and protein content in seeds, using imputed genotype data for 180 Eurasian soybean varieties and the novel “genotypic twins” approach. This dataset encompassed 87 Russian and European cultivars and 93 breeding lines from Western Siberia. We identified 11 novel loci significantly associated with oil and protein content in seeds (p-value < 1.5 × 10−6), including one locus on chromosome 11 linked to protein content and 10 loci associated with oil content (chromosomes 1, 5, 11, 16, 17, and 18). The protein-associated locus is located near a gene encoding a CBL-interacting protein kinase, which is involved in key biological processes, including stress response mechanisms such as drought and osmotic stress. The oil-associated loci were linked to genes with diverse functions, including lipid transport, nutrient reservoir activity, and stress responses, such as Sec14p-like phosphatidylinositol transfer proteins and Germin-like proteins. These findings suggest that the loci identified not only influence oil and protein content but may also contribute to plant resilience under environmental stress conditions. The data obtained from this study provide valuable genetic markers that can be used in breeding programs to optimize oil and protein content, particularly in varieties adapted to Russian climates, and contribute to the development of high-yielding, nutritionally enhanced soybean cultivars.
Shape Memory Performance and Microstructural Evolution in PLA/PEG Blends: Role of Plasticizer Content and Molecular Weight
Authors: Sringam Jiradet , Kajornprai Todsapol , Trongsatitkul Tatiya , Suppakarn Nitinat
Published: January 2025

Poly(lactic acid) (PLA) exhibits excellent shape memory properties but suffers from brittleness and a high glass transition temperature (Tg), limiting its utility in flexible and durable applications. This study explored the modification of PLA properties through the incorporation of poly(ethylene glycol) (PEG), varying in both content (5–20 wt%) and molecular weight (4000–12,000 g/mol), to enhance its suitability for specific applications, such as medical splints. The PLA/PEG blend, containing 15 wt% PEG and with a molecular weight of 12,000 g/mol, exhibited superior shape fixity (99.27%) and recovery (95.77%) in shape memory tests conducted at a programming temperature (Tp) of 45 °C and a recovery temperature (Tr) of 60 °C. Differential scanning calorimetry (DSC) analysis provided insights into the thermal mechanisms driving shape memory behavior of the PLA/PEG blend. The addition of PEG to the PLA blend resulted in a reduction in Tg and an increase in crystallinity, thereby facilitating enhanced chain mobility and structural reorganization. These thermal changes enhanced the shape fixity and recovery of the PLA/PEG blend. Synchrotron wide-angle X-ray scattering (WAXS) was further employed to elucidate the microstructural evolution of PLA/PEG blends during the shape memory process. Upon stretching, the PLA/PEG chains aligned predominantly along the tensile direction, reflecting strain-induced orientation. During recovery, the PLA/PEG chains underwent isotropic relaxation, reorganizing into their original configurations. This structural reorganization highlighted the critical role of chain mobility and alignment in driving the shape memory behavior of PLA/PEG blends, enabling them to effectively return to their initial shape. Mechanical testing confirmed that increasing PEG content and molecular weight enhanced elongation at break and impact strength, balancing flexibility and strength. These findings demonstrated that PLA/PEG blends, especially with 15 wt% PEG at 12,000 g/mol, offer an optimal combination of shape memory performance and mechanical properties, positioning them as promising candidates for customizable and biodegradable medical applications.
Molecular Dynamics Study of Polyacrylamide and Polysaccharide-Derived Flocculants Adsorption on Mg(OH)2 Surfaces at pH 11
Authors: Quezada Gonzalo R. , Vargas Antonia A. , Nieto Steven , García Karien I. , Robles Pedro , Jeldres Ricardo I.
Published: January 2025

Brucite (Mg(OH)2) is a typical precipitate in the mining industry that adversely affects processes such as flotation and thickening. Gaining insights into the physicochemical properties of this mineral is critical for developing strategies to mitigate these challenges and improve operational efficiency. Additionally, incorporating natural-origin polymers aligns with the shift toward more sustainable mining practices. In this study, molecular dynamics simulations were employed to investigate the interaction of brucite with polysaccharides such as cellulose, guar gum, and alginate and to compare these with conventional polymers, including polyacrylamide, hydrolyzed polyacrylamide, and polyacrylic acid, under conditions of pH 11 in low-salinity water. The methodology enhanced adsorption sampling by incorporating additional temporary interactions between the polymer and the brucite surface. The results reveal that neutral polymers exhibit stronger and more stable interactions with brucite compared to charged polymers, which is consistent with the neutral nature of brucite under the studied conditions. Van der Waals forces predominantly govern the adsorption of polysaccharides, while Coulombic forces primarily drive interactions involving polyacrylamides. These findings provide valuable insights into the molecular mechanisms of polymer-brucite interactions, facilitating the development of more effective and sustainable mining additives.