bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effects of the Pragmatic Intervention Programme in Children with Autism Spectrum Disorder and Developmental Language Disorder
Authors: Pereira Tatiana , Ramalho Ana Margarida , Valente Ana Rita S. , Couto Pedro Sá , Lousada Marisa
Published: November 2022

Purpose: Children with Autism Spectrum Disorder (ASD) and Developmental Language Disorder (DLD) frequently face pragmatic impairments which may result in learning, socialization, and mental health difficulties, therefore early intervention is crucial. In Portugal, the Pragmatic Intervention Programme (PICP) has been recently developed and validated, but its effects are unknown. This study aims to determine the effects of the PICP on preschool-age children with ASD or DLD with pragmatic impairments. Methods: A non-randomized controlled trial has been conducted. The children (n = 20) were assigned to the intervention (n = 11) or the control group (waiting list) (n = 9). Each child attended 24 PICP-based intervention sessions provided by a Speech and Language Therapist in kindergarten. The primary outcome measure was a Goal Attainment Scale (GAS) rated by parents and kindergarten teachers. Secondary outcomes include parent/teacher-reported communication skills (Escala de Avaliação de Competências Comunicativas) and an assessment of the child’s general language ability (Teste de Linguagem—Avaliação da Linguagem Pré-Escolar). Results: GAS results show that all the children in the intervention group made progress. Statistically significant differences between pre- and post-intervention assessments were found for all secondary outcomes. Conclusions: The main findings suggest that the PICP improves language in preschool-age children with ASD and DLD with pragmatic difficulties. Further research is needed to analyse the effects of the PICP for each neurodevelopmental disorder individually. These results are crucial and will contribute to future research and evidence-based practice.
Flat Slabs in Eccentric Punching Shear: Experimental Database and Code Analysis
Authors: Vargas Daniel , Lantsoght Eva O. L. , Genikomsou Aikaterini S.
Published: November 2022

Eccentric punching shear can occur in concrete slab–column connections when the connection is subjected to shear and unbalanced moments. Unbalanced moments occur in all floor slabs at the edge and corner columns. As such, this problem is of practical relevance. However, most punching experiments in the literature deal with concentric punching shear at internal columns. This paper presents a developed database of 128 experiments of flat slabs under eccentric punching shear, including a summary of the testing procedure of each reference and a description of the slab specimens. Additionally, a linear finite element analysis of all the specimens is included to determine the relevant sectional shear forces and moments. Finally, the ultimate shear stresses from the database experiments are compared to the shear capacities determined with ACI 318-19, Eurocode 2 and the Model Code 2010. The comparison shows that the Model Code 2010 is the most precise in the predictions with an average tested to predicted ratio of 0.82 and a coefficient of variation of 29.63%. It can be concluded that improvements to the current design methods for eccentric punching shear are necessary.
A Patient-Specific Fracture Risk Assessment Tool for Femoral Bone Metastases: Using the Bone Strength (BOS) Score in Clinical Practice
Authors: Eggermont Florieke , van der Linden Yvette , Verdonschot Nico , Dierselhuis Edwin , Ligthert Steven , Bitter Thom , Westhoff Paulien , Tanck Esther
Published: November 2022

Patients with femoral metastases are at risk of fracturing bones. It is important to prevent fractures in order to maintain mobility and quality of life. The BOne Strength (BOS) score is based on a computed tomography (CT)-based patient-specific finite element (FE) computer model that objectively calculates bone strength. In this pilot study, the added clinical value of the BOS score towards treatment-related decision making was assessed. In December 2019, the BOS score was implemented in four radiotherapy centers. The BOS scores and fracture risks of individual patients were calculated and returned to the physician to assist in treatment decisions. The physicians filled out a questionnaire, which was qualitatively analyzed. A follow-up to identify fractures and/or death was performed after six months. Until June 2021, 42 BOS scores were delivered (20 high, 9 moderate, and 13 low fracture risk). In 48%, the BOS score led to an adaptation of treatment plans. Physicians indicated that the BOS score provided objective insight into fracture risk, was reassuring for physicians and patients, and improved multidisciplinary discussions and shared decision making. In conclusion, the BOS score is an objective tool to assess fracture risk in femoral bone metastases and aids physicians and patients in making a more informed decision regarding the most appropriate treatment.
Anoctamins and Calcium Signalling: An Obstacle to EGFR Targeted Therapy in Glioblastoma?
Authors: Dewdney Brittany , Ursich Lauren , Fletcher Emily V. , Johns Terrance G.
Published: November 2022

Glioblastoma is the most common form of high-grade glioma in adults and has a poor survival rate with very limited treatment options. There have been no significant advancements in glioblastoma treatment in over 30 years. Epidermal growth factor receptor is upregulated in most glioblastoma tumours and, therefore, has been a drug target in recent targeted therapy clinical trials. However, while many inhibitors and antibodies for epidermal growth factor receptor have demonstrated promising anti-tumour effects in preclinical models, they have failed to improve outcomes for glioblastoma patients in clinical trials. This is likely due to the highly plastic nature of glioblastoma tumours, which results in therapeutic resistance. Ion channels are instrumental in the development of many cancers and may regulate cellular plasticity in glioblastoma. This review will explore the potential involvement of a class of calcium-activated chloride channels called anoctamins in brain cancer. We will also discuss the integrated role of calcium channels and anoctamins in regulating calcium-mediated signalling pathways, such as epidermal growth factor signalling, to promote brain cancer cell growth and migration.
Integrative Approaches to the Treatment of Cancer
Authors: O’Brien Kylie , Ried Karin , Binjemain Taufiq , Sali Avni
Published: November 2022

A significant proportion of cancer patients use forms of complementary medicine or therapies. An integrative approach to cancer management combines conventional medicine with evidence-based complementary medicines/therapies and lifestyle interventions, for the treatment and prevention of disease and the optimisation of health. Its basis is a holistic one; to treat the whole person, not just the disease. It makes use of adjunct technologies which may assist the clinician in diagnosis of early carcinogenesis and monitoring of treatment effectiveness. Many factors contribute to the development of cancer including some which are largely modifiable by the patient and which oncologists may be in a position to advise on, such as stress, poor nutrition, lack of physical activity, poor sleep, and Vitamin D deficiency. An integrative approach to addressing these factors may contribute to better overall health of the patient and better outcomes. Evidence-based complementary medicine approaches include the use of supplements, herbal medicine, various practices that reduce stress, and physical therapies. Individualised to the patient, these can also help address the symptoms and signs associated with cancer and its orthodox treatment.
Cu/CuOx@C Composite as a High-Efficiency Electrocatalyst for Oxygen Reduction Reactions
Authors: Zhang Ding , Li Yun-Fei , Liu Li-Xue , Duan Lei , Ren Zhi-Li , Xu Shou-Dong , Chen Liang , Guo Hui-Juan , Huang Yi , Shi Li-Juan , Yi Qun
Published: November 2022

Among clean energy transformation devices, fuel cells have gained special attention over the past years; however, advancing appropriate non-valuable metal impetuses to halfway supplant the customary Pt/C impetus is still in progress. In this paper, we propose a specific electrocatalyst in the formula of highly-active Cu species, associated with coated carbon (Cu@C-800), for oxygen reduction reaction (ORR) through post-treatment of a self-assembled precursor. The optimized catalyst Cu@C-800 showed excellent ORR performance (i.e., the onset potential was 1.00 V vs. RHE, and half-wave potential of 0.81 V vs. RHE), high stability, resistance to methanol, and high four-electron selectivity. The enhancement is attributed to the synergy between the carbon matrix and a high explicit surface region and rich Cu nano-species.
Electronic Tuning of Sterically Encumbered 2-(Arylimino)Pyridine-Nickel Ethylene Polymerization Catalysts by Para-Group Modification
Authors: Hosseinzadeh Zahra , Liu Ming , Zhang Qiuyue , Liang Tongling , Solan Gregory A. , Ma Yanping , Sun Wen-Hua
Published: November 2022

A collection of five related 2-(arylimino)pyridines, 2-{(2,6-(CH(C6H4-p-F)2)2-4- RC6H2)N=CMe}C5H4N, each ortho-substituted with 4,4′-difluorobenzhydryl groups but distinct in the electronic properties of the para-R substituent (R = Me L1, Et L2, i-Pr L3, F L4, OCF3 L5), were prepared and combined with (DME)NiBr2 to form their corresponding LNiBr2 complexes, Ni1–Ni5, in high yields. All the complexes were characterized by FT-IR, 19F NMR spectroscopy and elemental analysis, while Ni5 was additionally the subject of an X-ray determination, revealing a bromide-bridged dimer. The molecular structure of bis-ligated (L4)2NiBr2 (Ni4’) was also determined, the result of ligand reorganization having occurred during attempted crystallization of Ni4. On activation with either EtAlCl2 or MMAO, Ni1–Ni5 exhibited high catalytic activities (up to 4.28 × 106 g of PE (mol of Ni)−1 h−1 using EtAlCl2) and produced highly branched polyethylene exhibiting low molecular weight (Mw range: 2.50–6.18 kg·mol−1) and narrow dispersity (Mw/Mn range: 2.21–2.90). Notably, it was found that the type of para-R group impacted on catalytic performance with Ni5 > Ni4 > Ni3 > Ni1 > Ni2 for both co-catalysts, underlining the positive influence of electron withdrawing substituents. Analysis of the structural composition of the polyethylene by 1H and 13C NMR spectroscopy revealed the existence of vinyl-end groups (–CH=CH2) and high levels of internal unsaturation (–CH=CH–) (ratio of vinylene to vinyl, range: 3.1:1–10.3:1) along with various types of branch (Me, Et, Pr, Bu, 1,4-paired Me, 1,6-paired Me and LCBs). Furthermore, reaction temperature was shown to greatly affect the end group type, branching density, molecular weight and in turn the melting points of the resulting polyethylenes.
Photocatalytic Degradation of 4-tert-butylphenol Using Solar Light Responsive Ag2CO3
Authors: Mergenbayeva Saule , Atabaev Timur Sh. , Vakros John , Mantzavinos Dionissios , Poulopoulos Stavros G.
Published: November 2022

In this work, Ag2CO3 was prepared via a solution-based method and was further characterized by XRD, Raman spectroscopy, SEM/EDS analysis, and UV-VIS spectroscopy. SEM results revealed the formation of micro-sized particles with a rectangular shape. The photocatalytic activity of the catalyst was evaluated in the degradation of 4-tert-butylphenol (4-t-BP) under simulated solar light irradiation. The effects of 4-t-BP initial concentration (2.5–10 ppm), catalyst dosage (100–300 mg/L), different types of lamp sources, and water matrix were investigated. Complete 4-t-BP (5 ppm) degradation was achieved after 60 min by Ag2CO3 (200 mg/L). The effect of anions such as CO32−, HCO3−, NO3−, and Cl- in the concentration range of 100–300 mg/L was also studied. CO32− promoted the photocatalytic degradation process, while HCO3− and NO3− exhibited an inhibition effect, which was marked with increasing HCO3− and NO3− concentrations. The presence of Cl− at the concentration of 100 mg/L increased 4-t-BP degradation, but higher concentrations inhibited the photocatalytic reaction. Cyclic experiments showed that the catalyst practically retained its catalytic activity toward 4-t-BP degradation after three successive experimental runs.
Hydrogen Production through Oxidative Steam Reforming of Acetic Acid over Ni Catalysts Supported on Ceria-Based Materials
Authors: Megía Pedro J. , Morales Anabel , Vizcaíno Arturo J. , Calles José A. , Carrero Alicia
Published: November 2022

Oxidative steam reforming allows higher energy efficiency and lowers coke deposition compared to traditional steam reforming. In this work, CeO2-based supports have been prepared with Ni as the active phase, and they were tested in the oxidative steam reforming of acetic acid. The influence of the O2/AcOH molar ratio (0–0.3) has been evaluated over Ni/CeO2. The results stated that by increasing oxygen content in the feeding mixture, acetic acid conversion increases, too, with a decrease in coke deposition and hydrogen yield. To have a proper balance between the acetic acid conversion and the hydrogen yield, an O2/AcOH molar ratio of 0.075 was selected to study the catalytic performance of Ni catalysts over different supports: commercial CeO2, a novel mesostructured CeO2, and CeO2-SBA-15. Due to higher Ni dispersion over the support, the mesostructured catalysts allowed higher acetic acid conversion and hydrogen yield compared to the nonporous Ni/CeO2. The best catalytic performance and the lowest coke formation (120.6 mgcoke·gcat−1·h−1) were obtained with the mesostructured Ni/CeO2. This sample reached almost complete conversion (>97%) at 500 °C, maintaining the hydrogen yield over 51.5% after 5 h TOS, being close to the predicted value by the thermodynamic equilibrium that is due to the synergistic coordination between Ni and CeO2 particles.
Band-Gap Engineering of Layered Perovskites by Cu Spacer Insertion as Photocatalysts for Depollution Reaction
Authors: Raciulete Monica , Anastasescu Crina , Papa Florica , Atkinson Irina , Bradu Corina , Negrila Catalin , Eftemie Diana-Ioana , Culita Daniela C. , Miyazaki Akane , Bratan Veronica , Pandele-Cusu Jeanina , Munteanu Cornel , Dobrescu Gianina , Sandulescu Alexandra , Balint Ioan
Published: November 2022

A multi-step ion-exchange methodology was developed for the fabrication of Cu(LaTa2O7)2 lamellar architectures capable of wastewater depollution. The (001) diffraction line of RbLaTa2O7 depended on the guest species hosted by the starting material. SEM and TEM images confirmed the well-preserved lamellar structure for all intercalated layered perovskites. The UV–Vis, XPS, and photocurrent spectroscopies proved that Cu intercalation induces a red-shift band gap compared to the perovskite host. Moreover, the UV–Vis spectroscopy elucidated the copper ions environment in the Cu-modified layered perovskites. H2-TPR results confirmed that Cu species located on the surface are reduced at a lower temperature while those from the interlayer occur at higher temperature ranges. The photocatalytic degradation of phenol under simulated solar irradiation was used as a model reaction to assess the performances of the studied catalysts. Increased photocatalytic activity was observed for Cu-modified layered perovskites compared to RbLaTa2O7 pristine. This behavior resulted from the efficient separation of photogenerated charge carriers and light absorption induced by copper spacer insertion.
Influence of Oxidative Properties of CexZr1−xO2 Catalyst on Partial Oxidation of Dimethyl Ether
Authors: Fu Zhu , Qi Ping , Liu Huimin , Zhang Qijian , Zhao Yonghua , Feng Xiaoqian
Published: November 2022

Partial oxidation of dimethyl ether (DME) to hydrogen is an efficient route for hydrogen production for application in SOFC. However, quite a large amount of CO2 as a byproduct has been an important obstacle. In this work, cerium–zirconium solid solution (CexZr1−xO2) was applied to determine how the oxidative properties of the catalyst affect the production of CO2 in the partial oxidation of DME. The results show that the catalyst with more adsorbed oxygen (Oads) rather than the lattice oxygen has stronger oxidizability and gives higher DME conversion as well as higher CO2 yield, due to the overoxidation of CO.
Emission Spectroscopy of CH4/CO2 Mixtures Processed in a Non-Thermal Plasma Augmented Burner
Authors: Bykov Ernest , Striūgas Nerijus , Paulauskas Rolandas
Published: November 2022

The need for energy resources that do not belong to the group of fossil fuels and a wide availability of various low-calorific gases leads humanity to search for solutions to adapt external sources of force that would allow for the use of these resources. One of such solutions is the usage of non-thermal plasma applications for pre-ignition stage, ignition, and, finally, combustion. Plasma assistance is a promising technology for improving processes of ignition and flame stabilization, as well as propagating flame speed. This study focuses on influence of the non-thermal plasma on both for CH4, CO2 gases, and their mixture in pre-ignition stage by performing emission spectroscopy, and determining tendency of excited species at different frequency rates for optimal plasma parameters to reduce NO formation and increase efficiency during combustion. The results obtained exhibit a non-linear dependence of radical’s emission from the frequency of plasma. As an example of possible profits from correctly choosing plasma parameters, the calorific value of gases increased from 2.86 times for BG25/75 to 4.78 times for BG30/70. However, the decomposition on higher frequencies causes higher rates of nitrogen-bands emissions, which would increase NOx emissions in the combustion process.
Highly Dispersed Nickel Nanoparticles on Hierarchically Ordered Macroporous Al2O3 and Its Catalytic Performance for Steam Reforming of 1-Methyl Naphthalene
Authors: Do Lien Thi , Nguyen-Phu Huy , Pham Ngoc Nhiem , Jeong Dong Hwi , Shin Eun Woo
Published: November 2022

In this study, we investigate the effect of a hierarchically ordered macroporous structure of alumina support on the steam reforming of 1-methyl naphthalene with mesoporous alumina-supported nickel and potassium (xK/Ni–MeAl), and macroporous alumina-supported nickel and potassium (xK/Ni–MaAl) catalysts. Hierarchically ordered macroporosity in Al2O3 supports plays an important role in maintaining the high Ni dispersion through multiple interactions in Ni–K over AlO4 tetrahedra in alumina. This, in turn, improves the catalytic performance of steam reforming, including high gas yields, turnover frequency for hydrogen production, and 1-methyl naphthalene conversion. At high K content, the Ni active sites over xK/Ni–MeAl catalysts significantly decrease, resulting in almost zero steam reforming rate in the reaction test. Conversely, the potassium–alumina interaction in xK/Ni–MaAl catalysts not only diminishes the formation of the inactive nickel aluminate phase but also maintains the highly dispersed Ni active sites, resulting in a high steam reforming rate.
Dying of Stress: Chemotherapy, Radiotherapy, and Small-Molecule Inhibitors in Immunogenic Cell Death and Immunogenic Modulation
Authors: Fabian Kellsye P. , Kowalczyk Joshua T. , Reynolds Sandy T. , Hodge James W.
Published: November 2022

Innovative strategies to re-establish the immune-mediated destruction of malignant cells is paramount to the success of anti-cancer therapy. Accumulating evidence suggests that radiotherapy and select chemotherapeutic drugs and small molecule inhibitors induce immunogenic cell stress on tumors that results in improved immune recognition and targeting of the malignant cells. Through immunogenic cell death, which entails the release of antigens and danger signals, and immunogenic modulation, wherein the phenotype of stressed cells is altered to become more susceptible to immune attack, radiotherapies, chemotherapies, and small-molecule inhibitors exert immune-mediated anti-tumor responses. In this review, we discuss the mechanisms of immunogenic cell death and immunogenic modulation and their relevance in the anti-tumor activity of radiotherapies, chemotherapies, and small-molecule inhibitors. Our aim is to feature the immunological aspects of conventional and targeted cancer therapies and highlight how these therapies may be compatible with emerging immunotherapy approaches.
Circulating Small Extracellular Vesicle-Derived miR-342-5p Ameliorates Beta-Amyloid Formation via Targeting Beta-site APP Cleaving Enzyme 1 in Alzheimer’s Disease
Authors: Dong Zhiwu , Gu Hongjun , Guo Qiang , Liu Xianglu , Li Feifei , Liu Huiling , Sun Li , Ma Huimin , Zhao Kewen
Published: November 2022

Alzheimer’s disease (AD) is a common neurodegenerative disorder with progressive cognitive impairment in the elderly. Beta-amyloid (Aβ) formation and its accumulation in the brain constitute one of the pathological hallmarks of AD. Until now, how to modulate Aβ formation in hippocampal neurons remains a big challenge. Herein, we investigated whether the exosomal transfer of microRNA (miR) relates to amyloid pathology in the recipient neuron cells. We isolated circulating small extracellular vesicles (sEVs) from AD patients and healthy controls, determined the miR-342-5p level in the sEVs by RT-PCR, and evaluated its diagnostic performance in AD. Then, we took advantage of biomolecular assays to estimate the role of miR-342-5p in modulating the amyloid pathway, including amyloid precursor protein (APP), beta-site APP cleaving enzyme 1 (BACE1), and Aβ42. Furthermore, we subjected HT22 cells to the sEVs from the hippocampal tissues of transgenic APP mice (Exo-APP) or C57BL/6 littermates (Exo-CTL), and the Exo-APP enriched with miR-342-5p mimics or the control to assess the effect of the sEVs’ delivery of miR-342-5p on Aβ formation. We observed a lower level of miR-342-5p in the circulating sEVs from AD patients compared with healthy controls. MiR-342-5p participated in Aβ formation by modulating BACE1 expression, specifically binding its 3′-untranslated region (UTR) sequence. Exo-APP distinctly promoted Aβ42 formation in the recipient cells compared to Exo-CTL. Intriguingly, miR-342-5p enrichment in Exo-APP ameliorated amyloid pathology in the recipient cells. Our study indicated that miR-342-5p was dysregulated in human circulating sEVs from AD patients; sEV transfer of miR-342-5p ameliorates Aβ formation by modulating BACE1 expression. These findings highlight the promising potential of exosomal miRNAs in AD clinical therapy.
Beneficial Effects of Hyaluronan-Based Hydrogel Implantation after Cortical Traumatic Injury
Authors: Lainé Anaïs , Brot Sébastien , Gaillard Afsaneh
Published: November 2022

Traumatic brain injury (TBI) causes cell death mainly in the cerebral cortex. We have previously reported that transplantation of embryonic cortical neurons immediately after cortical injury allows the anatomical reconstruction of injured pathways and that a delay between cortical injury and cell transplantation can partially improve transplantation efficiency. Biomaterials supporting repair processes in combination with cell transplantation are in development. Hyaluronic acid (HA) hydrogel has attracted increasing interest in the field of tissue engineering due to its attractive biological properties. However, before combining the cell with the HA hydrogel for transplantation, it is important to know the effects of the implanted hydrogel alone. Here, we investigated the therapeutic effect of HA on host tissue after a cortical trauma. For this, we implanted HA hydrogel into the lesioned motor cortex of adult mice immediately or one week after a lesion. Our results show the vascularization of the implanted hydrogel. At one month after HA implantation, we observed a reduction in the glial scar around the lesion and the presence of the newly generated oligodendrocytes, immature and mature neurons within the hydrogel. Implanted hydrogel provides favorable environments for the survival and maturation of the newly generated neurons. Collectively, these results suggest a beneficial effect of biomaterial after a cortical traumatic injury.
Hypoxia-Induced HIF-1α Expression Promotes Neurogenic Bladder Fibrosis via EMT and Pyroptosis
Authors: Li Qi , Hong Yifan , Chen Jing , Zhou Xiazhu , Tian Xiaomao , Yu Yihang , Shen Lianju , Long Chunlan , Cai Miao , Wu Shengde , Wei Guanghui
Published: November 2022

Background: Neurogenic bladder (NB) patients exhibit varying degrees of bladder fibrosis, and the thickening and hardening of the bladder wall induced by fibrosis will further affect bladder function and cause renal failure. Our study aimed to investigate the mechanism of bladder fibrosis caused by a spinal cord injury (SCI). Methods: NB rat models were created by cutting the bilateral lumbar 6 (L6) and sacral 1 (S1) spinal nerves. RNA-seq, Western blotting, immunofluorescence, cell viability and ELISA were performed to assess the inflammation and fibrosis levels. Results: The rats showed bladder dysfunction, upper urinary tract damage and bladder fibrosis after SCI. RNA-seq results indicated that hypoxia, EMT and pyroptosis might be involved in bladder fibrosis induced by SCI. Subsequent Western blot, ELISA and cell viability assays and immunofluorescence of bladder tissue confirmed the RNA-seq findings. Hypoxic exposure increased the expression of HIF-1α and induced EMT and pyroptosis in bladder epithelial cells. Furthermore, HIF-1α knockdown rescued hypoxia-induced pyroptosis, EMT and fibrosis. Conclusion: EMT and pyroptosis were involved in the development of SCI-induced bladder fibrosis via the HIF-1α pathway. Inhibition of the HIF-1α pathway may serve as a potential target to alleviate bladder fibrosis caused by SCI.
Discovery of 16-Androstenes (Androstenone and Androstenol), Their Synthesis Pathway, and Possible Role in Reproduction of Mouse Deer (Moschiola indica)
Authors: Kumar Vinod , Manu Shivakumara , Caroline Karunakaran , Sekhar Anupama , Mamta Sajwan-Khatri , Sandeep Mushkam , . Wasimuddin , Senthilkumaran Balasubramanian , Umapathy Govindhaswamy
Published: November 2022

We discovered odorous 16-androstenes (Androstenone and Androstenol) in endangered mouse deer during a captive breeding program. This study examined the molecular characteristics, their synthesis pathway, and the possible functional role of these compounds in the reproduction of mouse deer. CYP17A1 and CYB5 genes were cloned and expressed in HEK-293, COS-7 cell lines, and gonads of mouse deer to investigate the CYP17A1 gene’s andien-β-synthase activity towards the synthesis of 16-androstenes in mouse deer. An enzyme immunoassay was further developed and standardized to measure fecal androstenone during the reproductive cycles of mouse deer. Results showed that the mouse deer CYP17A1 gene possesses andien-β-synthase activity and could transform pregnenolone into 5,16-androstadien-3β-ol. The expression of the CYP17A1 gene upregulated in the testis and ovary compared to other tissues in mouse deer. Significantly elevated androstenone and estrogens were recorded prior to delivery and postpartum estrus/mating in mouse deer. Further, there were weak correlations between fecal androstenone and estrogens/androgens in mouse deer during the breeding season. These findings suggest that androstenone probably plays a role in the reproductive activities of mouse deer. This knowledge can be used for captive breeding programs of mouse deer in India and elsewhere.
CAR Triggered Release of Type-1 Interferon Limits CAR T-Cell Activities by an Artificial Negative Autocrine Loop
Authors: Harrer Dennis Christoph , Schenkel Charlotte , Bezler Valerie , Kaljanac Marcell , Hartley Jordan , Barden Markus , Pan Hong , Holzinger Astrid , Herr Wolfgang , Abken Hinrich
Published: November 2022

The advent of chimeric antigen receptor (CAR) T cells expedited the field of cancer immunotherapy enabling durable remissions in patients with refractory hematological malignancies. T cells redirected for universal cytokine-mediated killing (TRUCKs), commonly referred to as “fourth generation” CAR T-cells, are designed to release engineered payloads upon CAR-induced T-cell activation. Building on the TRUCK technology, we aimed to generate CAR T-cells with a CAR-inducible artificial, self-limiting autocrine loop. To this end, we engineered CAR T-cells with CAR triggered secretion of type-1 interferons (IFNs). At baseline, IFNα and IFNβ CAR T-cells showed similar capacities in cytotoxicity and cytokine secretion compared to conventional CAR T-cells. However, under “stress” conditions of repetitive rounds of antigen stimulation using BxPC-3 pancreas carcinoma cells as targets, anti-tumor activity faded in later rounds while being fully active in destructing carcinoma cells during first rounds of stimulation. Mechanistically, the decline in activity was primarily based on type-1 IFN augmented CAR T-cell apoptosis, which was far less the case for CAR T-cells without IFN release. Such autocrine self-limiting loops can be used for applications where transient CAR T-cell activity and persistence upon target recognition is desired to avoid lasting toxicities.
Transcriptomic Classification of Pituitary Neuroendocrine Tumors Causing Acromegaly
Authors: Rymuza Julia , Kober Paulina , Rusetska Natalia , Mossakowska Beata J. , Maksymowicz Maria , Nyc Aleksandra , Baluszek Szymon , Zieliński Grzegorz , Kunicki Jacek , Bujko Mateusz
Published: November 2022

Acromegaly results from growth hormone hypersecretion, predominantly caused by a somatotroph pituitary neuroendocrine tumor (PitNET). Acromegaly-causing tumors are histologically diverse. Our aim was to determine transcriptomic profiles of various somatotroph PitNETs and to evaluate clinical implication of differential gene expression. A total of 48 tumors were subjected to RNA sequencing, while expression of selected genes was assessed in 134 tumors with qRT-PCR. Whole-transcriptome analysis revealed three transcriptomic groups of somatotroph PitNETs. They differ in expression of numerous genes including those involved in growth hormone secretion and known prognostic genes. Transcriptomic subgroups can be distinguished by determining the expression of marker genes. Analysis of the entire cohort of patients confirmed differences between molecular subtypes of tumors. Transcriptomic group 1 includes ~20% of acromegaly patients with GNAS mutations-negative, mainly densely granulated tumors that co-express GIPR and NR5A1 (SF-1). SF-1 expression was verified with immunohistochemistry. Transcriptomic group 2 tumors are the most common (46%) and include mainly GNAS-mutated, densely granulated somatotroph and mixed PitNETs. They have a smaller size and express favorable prognosis-related genes. Transcriptomic group 3 includes predominantly sparsely granulated somatotroph PitNETs with low GNAS mutations frequency causing ~35% of acromegaly. Ghrelin signaling is implicated in their pathogenesis. They have an unfavorable gene expression profile and higher invasive growth rate.
Relative Leukocyte Telomere Length and Genetic Variants in Telomere-Related Genes and Serum Levels Role in Age-Related Macular Degeneration
Authors: Vilkeviciute Alvita , Gedvilaite Greta , Banevicius Mantas , Kriauciuniene Loresa , Zaliuniene Dalia , Dobiliene Olivija , Liutkeviciene Rasa
Published: November 2022

Telomere shortening is well known to be associated with ageing. Age is the most decisive risk factor for age-related macular degeneration (AMD) development. The older the individual, the higher the AMD risk. For this reason, we aimed to find any associations between telomere length, distribution of genetic variants in telomere-related genes (TERT, TERT-CLPTM1, TRF1, TRF2, and TNKS2), and serum TERF-1 and TERF2 levels on AMD development. Methods: Our study enrolled 342 patients with AMD and 177 healthy controls. Samples of DNA from peripheral blood leukocytes were extracted by DNA salting-out method. The genotyping of TERT rs2736098, rs401681 in TERT-CLPTM1 locus, TRF1 rs1545827, rs10107605, TNKS2 rs10509637, rs10509639, and TRF2 rs251796 and relative leukocyte telomere length (T/S) measurement were carried out using the real-time polymerase chain reaction method. Serum TERF-1 and TERF2 levels were measured by enzymatic immunoassay (ELISA). Results: We found longer telomeres in early AMD patients compared to the control group. Additionally, we revealed that minor allele C at TRF1 rs10107605 was associated with decreases the odds of both early and exudative AMD. Each minor allele G at TRF2 rs251796 and TRF1 rs1545827 C/T genotype and C/T+T/T genotypes, compared to the C/C genotype, increases the odds of having shorter telomeres. Furthermore, we found elevated TERF1 serum levels in the early AMD group compared to the control group. Conclusions: In conclusion, these results suggest that relative leukocyte telomere length and genetic variants of TRF1 and TRF2 play a role in AMD development. Additionally, TERF1 is likely to be associated with early AMD.
Advances in Musculoskeletal Cell Therapy: Basic Science and Translational Approaches
Authors: Ragni Enrico , Colombini Alessandra , Lopa Silvia
Published: November 2022

Nowadays, the real need in orthopedic research is to strictly validate advanced regenerative medicine approaches in preclinical models, with the hope that this unique and straightforward approach can facilitate a safe and effective translation into everyday clinical practice [...]
Estimation of Serum Calcium on the Severity and Mortality in COVID-19 Infections in Sulaymaniyah City, Kurdistan Region of Iraq: A Cross-Sectional Study
Authors: Hadi Jihad M. , Hassan Shkar M. J. , Saeed Mudhafar M. M. , Hussein Bilal K. , Ali Banwan M. , Muhamad Lava E. , Abdullah Ardalan J. , Ali Nzar N. , Rahman Hawre A. , Sofihussein Hassan Q. , Abdul Aziz Jeza M.
Published: November 2022

Background: Coronaviruses (COV) are a large family of viruses that cause infections ranging from the common cold to more serious diseases. Mild to severe respiratory illnesses have been linked to coronavirus disease 2019 (COVID-19), which has been classified as a pandemic disease by the World Health Organization. It has been demonstrated that the severity of COVID-19 is highly positively linked with hypocalcemia. Furthermore, calcium imbalances among other electrolytes are linked to the prognosis of COVID-19. Objectives: This study demonstrates a connection between serum calcium levels and COVID-19 as biomedical indicators of COVID-19 infections in Sulaymaniyah city, Iraq. Methods: A cross-sectional study was conducted at Baxshin Hospital for about two months from February 2022 to April 2022. The work was conducted with a total of 40 patients including 22 males and 18 females. The patients’ ages ranged from 22 to 80 years old. By analyzing a sample from a nasopharyngeal swab and performing real-time reverse transcription-polymerase chain reaction (RT-PCR), all of the patients tested positive as having COVID-19 infection. Serum calcium was determined from the blood samples of the patients in order to evaluate their serum calcium levels. The statistical package for social science (SPSS) was utilized to examine the obtained data. Results: The study revealed a level of calcium between 6.10 and 9.86 mg/dL in male and female patients. The majority of the female patients (61%) displayed low levels of serum calcium, and 33% of the males had a low level of calcium. It can be seen that the highest rate of male patients (66.6%) exhibited a normal level of serum calcium, while 33.3% showed decreased serum calcium. Based on gender and age groups, a statistically significant difference in calcium levels was observed. Conclusions: This study discovered that infection with COVID-19 has some significant laboratory abnormalities, including hypocalcemia, showing that serum calcium might be employed as a prognostic marker in the clinic.
Nozzle Geometry and Particle Size Influence on the Behavior of Low Pressure Cold Sprayed Hydroxyapatite Particles
Authors: Forero-Sossa Paola Andrea , Giraldo-Betancur Astrid Lorena , Poblano-Salas Carlos A. , Gutierrez-Pérez Aixa Ibeth , Rodríguez-Vigueras Esaú Moises , Corona-Castuera Jorge , Henao John
Published: November 2022

Low-pressure cold spray (LPCS) technology has attracted interest for the deposition of ceramic coatings due to the thermo-kinetic conditions experienced by the sprayed particles. Unlike conventional thermal spray techniques, the spraying conditions in LPCS can be controlled to avoid the formation of undesired phases. However, ceramics deposition through this process is still challenging. The present study includes a finite element analysis and simulation study of the kinetic conditions of ceramic particles in the LPCS process based on experimental data. The analysis seeks to discuss the effect of nozzle geometry on the kinetic and thermal energy of the sprayed particles at impact and elucidate how the particle travels within the high-velocity jet to be deposited onto a metallic surface. This work examines the behavior of hydroxyapatite particles as a function of particle size and nozzle geometry during LPCS deposition. Interestingly, the results from this research suggest that particle size and nozzle geometry have an influence on the deposition of hydroxyapatite particles. Inertia of large particles proved to be beneficial in keeping their trajectories, allowing them to contribute to the formation of the coatings. Nozzle geometry modifications produced changes in the jet profile and affected the homogeneity of the coatings obtained. This finding contributes to a better understanding of the deposition of hydroxyapatite particles by cold spraying.
Metastable Phase Formation, Microstructure, and Dielectric Properties in Plasma-Sprayed Alumina Ceramic Coatings
Authors: Junge Paul , Greinacher Moritz , Kober Delf , Stargardt Patrick , Rupprecht Christian
Published: November 2022

The need for new solutions for electrical insulation is growing due to the increased electrification in numerous industrial sectors, opening the door for innovation. Plasma spraying is a fast and efficient way to deposit various ceramics as electrical insulators, which are used in conditions where polymers are not suitable. Alumina (Al2O3) is among the most employed ceramics in the coating industry since it exhibits good dielectric properties, high hardness, and high melting point, while still being cost-effective. Various parameters (e.g., feedstock type, spray distance, plasma power) significantly influence the resulting coating in terms of microstructure, porosity, and metastable phase formation. Consequently, these parameters need to be investigated to estimate the impact on the dielectric properties of plasma-sprayed alumina coatings. In this work, alumina coatings with different spray distances have been prepared via atmospheric plasma spray (APS) on copper substrates. The microstructure, porosity, and corresponding phase formation have been analyzed with optical microscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM). Moreover, we present an in-depth analysis of the fundamental dielectric properties e.g., direct current (DC) resistance, breakdown strength, dielectric loss tangent, and permittivity. Our results show that decreasing spray distance reduces the resistivity from 6.31 × 109Ωm (130 mm) to 6.33 × 108Ωm (70 mm), while at the same time enhances the formation of the metastable δ-Al2O3 phase. Furthermore, space charge polarization is determined as the main polarization mechanism at low frequencies.
Rheological Properties and Microstructural Analysis of Asphalt Binder Doped with Anti-Stripping Agent after Aging
Authors: Liu Keyan , Xu Yongli , Gao Dapeng , Yang Songhan
Published: November 2022

In order to comprehensively explore the long-term durability of asphalt mixed with an anti-stripping agent, laboratory tests were carried out on base asphalt, SBS modified asphalt and base asphalt mixed with an anti-stripping agent after long-term aging. The rheological properties, chemical composition and microscopic morphology of the three asphalts before and after aging were compared and analyzed from macroscopic and microscopic angles. The results show that the modulus of asphalt is more affected by temperature than aging, and the anti-aging ability of the asphalt with the anti-stripping agent is better than that of base asphalt and slightly worse than that of SBS modified asphalt. The change in the absorption peak intensity before and after the aging of asphalt with the anti-stripping agent is the same as those of the base asphalt and SBS modified asphalt at 2920 cm−1 and 2850 cm−1, while the opposite pattern is observed in other four places. The honeycomb structures of the base asphalt and SBS modified asphalt became longer and the number of peaks increased slightly after aging. However, the asphalt surface with the anti-stripping agent had no obvious honeycomb structures before aging, and there were obvious honeycomb structures after aging, the number of which was between those of the base asphalt and SBS modified asphalt after aging; moreover, the lengths of honeycomb structures were still significantly smaller than those of the base asphalt after aging. This study investigated the performance changes of the anti-stripping-agent asphalt before and after long-term aging at multiple scales and compared the asphalt mixed with the anti-stripping agent with base asphalt and SBS modified asphalt to fully evaluate its long-term durability.
Preparation and Characterisation of UV-Curable Flame Retardant Wood Coating Containing a Phosphorus Acrylate Monomer
Authors: Pellerin Solène , Samyn Fabienne , Duquesne Sophie , Landry Véronic
Published: November 2022

The application of a flame retardant coating is an effective solution to enhance the fire retardancy of wood flooring. However, finding the right balance between reducing the flame propagation and good overall coating properties while conserving wood appearance is complex. In order to answer this complex problem, transparent ultraviolet (UV)-curable flame retardant wood coatings were prepared from an acrylate oligomer, an acrylate monomer, and the addition of the tri(acryloyloxyethyl) phosphate (TAEP), a phosphorus-based monomer, at different concentrations in the formulation. The coatings’ photopolymerisation, optical transparency, hardness, water sorption and thermal stability were assessed. The fire behaviour and the adhesion of the coatings applied on the yellow birch panels were evaluated, respectively, using the cone calorimeter and pull-off tests. Scanning electron microscopy (SEM) and energy-dispersive spectroscopy (EDS) analyses were performed on the collected burnt residues to obtain a better understanding of the flame retardancy mechanism. Our study reveals that phosphorus monomer addition improved the coating adhesion and the fire performance of the coated wood without impacting the photopolymerisation. The conversion percentage remained close to 70% with the TAEP addition. The pull-off strength reached 1.12 MPa for the coating with the highest P-monomer content, a value significantly different from the non-flame retarded coating. For the same coating formulation, the peak of heat release rate decreased by 13% and the mass percentage of the residues increased by 37% compared to the reference. However, the flame-retarded coatings displayed a higher hygroscopy. The action in the condensed phase of the phosphorus flame retardant is highlighted in this study.
Preparation of a Poly (Ether-b-Amide) Mixed-Matrix Membrane and Its Application in Blast Furnace Gas
Authors: Jiang Lili , Chen Yixing , Hou Xingang
Published: November 2022

In this paper, we propose the use of nanomaterials to build three-dimensional structured gas-separation membranes to accommodate more gas molecules. SWCNT provides a fast passage for gas molecules, while GO can make gas molecules have more adsorption sites due to its large specific surface and the fact that its special lamellar folds can hinder the transport of macromolecules. Graphene oxide (GO) was prepared by an improved Hummer’s method, and single-walled carbon nanotubes (SWCNTs) were purified by mixed acid treatment. Then, Pebax1657 particles were dissolved in a reflux setup at 80 °C, and GO/SWCNT/Pebax1657 mixed-matrix membrane (MMM) was prepared by solution blending. Finally, the permeability and selectivity of GO/SWCNT/Pebax1657 membrane to single gas and mixed gas at different temperature and inlet pressure were investigated.
Wetting Patterns of Liquid-Repellent Femtosecond Laser Textured Aluminum Surfaces
Authors: Myronyuk Oleksiy , Baklan Denys , Vasilyev Georgii S. , Rodin Aleksej M. , Vanagas Egidijus
Published: November 2022

Although liquid-repellent surfaces are in demand in many applications, their use is limited by the Cassie state’s sustainability to environmental factors, such as the repellency of liquids with a surface tension lower than that of water. This phenomenon remains not fully understood, despite a well-developed theory. In the current work, wetting of femtosecond laser-textured aluminum surfaces for probe liquids with a surface tension of 72.8–21.2 mN/m was considered. The resulting patterns were an array of pillars at the micro level and fractal-like structures at the nano level. These structured surfaces were treated with alkoxysilanes and oleic acid. All textured samples exhibited a Cassie state with water, and contact angles greater than 150° were achieved with silane-treated surfaces. A decrease in the surface tension of the probe liquid led to а transition to the Wetzel state at 44–46 mN/m for alkoxysilanes and at 52 mN/m for oleic acid. A typical shape of the textured surface wetting curve is proposed. It was shown that the determined values of the surface tension of the Cassie-Wenzel transition were 10–15 mN/m lower than those predicted by the Cassie equations.
Effect of Standoff Distance on Corrosion Resistance of Cold Sprayed Titanium Coatings
Authors: Scendo Mieczyslaw , Staszewska-Samson Katarzyna
Published: November 2022

A titanium protective layer was deposited onto Al7075 substrate (Al7075/Ti) by a cold spray method (CS) with different standoff distances (SoD) of the nozzle from the specimen surface. The aim of this research was to study the influence on the mechanical properties and corrosion resistance of the Ti coating on Al7075 substrate. The surface and microstructure of Al7075/Ti was observed by a scanning electron microscope (SEM). The corrosion test of the materials was carried out by using the electrochemical method. The SoD had a significant effect on the microstructure of the coatings and their adhesion onto Al7075 substrate. The highest level of microhardness (248 HV0.3) value was achieved for deposits obtained with the SoD of 70 mm. The corrosion tests showed that the mechanism of electrochemical corrosion of titanium coatings is a multi-stage process, and the main product of the corrosion process was (TiO2)ads. However, the polarization resistance (Rp = 49 kΩ cm2) of the Al7075/Ti coatings was the highest, while the corrosion rate (υcorr = 13.90 mm y−1) was the lowest, for SoD of 70 mm.