bluuebunny/crossref_metadata_2025 huggingface dataset:
How to foster the commitment level of managers? Exploring the role of moderators on the relationship between job satisfaction and organizational commitment: A study of educational managers in Vietnam
Authors: Huynh Tho H. P. , Bui Thong Quang , Nguyen Phuong N. D.
Published: March 2023

Organizational commitment is a perennial concern. Numerous studies have been conducted to figure out how to strengthen the commitment of employees to the organization. Many of them examined the moderating factors that influence the link between job satisfaction and organizational commitment. However, the majority of past research has focused on the private and corporate sectors. Further, some management personnel opt to quit the firm while still being content with their existing position. Therefore, this study aims to investigate the role of moderators in the relationship between job satisfaction and organizational commitment by applying the purposive sampling method to conduct the data survey of 402 managers in Ho Chi Minh City, Vietnam, who work in the public education sector. By using the PLS-SEM modeling approach with the help of SmartPLS 4 software to examine the research hypotheses, the results reveal that organizational commitment is positively affected by job satisfaction and work engagement. At the same time, managers with high person-organization fit and fun at work will have higher levels of organizational commitment in situations of high satisfaction in their jobs. Conversely, high role stress will dampen the levels of organizational commitment in situations of high job satisfaction. The results offered some managerial implications to strengthen the commitment to the organization of the managers in the educational sector. This research also stressed some limitations and suggested opportunities for future investigation.
Bromocriptine inhibits proliferation in the endometrium from women with adenomyosis
Authors: Tang Yiqun , Ponandai-srinivasan Sakthivignesh , Frisendahl Caroline , Andersson Johanna K. , Pavone Dora , Stewart Elizabeth A. , Lalitkumar Parameswaran Grace Luther , Korsching Eberhard , Bogavarappu Nageswara Rao , Gemzell-Danielsson Kristina
Published: March 2023

Objective

Bromocriptine treatment has been shown to reduce menstrual bleeding and pain in women with adenomyosis in a pilot clinical trial. The underlying mechanism contributing to the treatment effect is however unknown. The purpose of this study was to explore the effect of bromocriptine on the proliferation and migration properties of the endometrium in women with adenomyosis, by assessing cellular and molecular changes after six months of vaginal bromocriptine treatment.

Methods

Endometrial specimens were collected during the proliferative phase from women with adenomyosis (n=6) before (baseline) and after six months of treatment with vaginal bromocriptine. Immunohistochemistry was used to determine changes in the protein expression of Ki67 in the endometrium of women with adenomyosis. Primary endometrial stromal cells isolated at baseline were expanded in vitro and exposed to different doses of bromocriptine to determine the optimal half-maximum inhibitory concentration (IC50) using CellTiter-Blue® Cell Viability Assay. Cell proliferation was assessed by bromodeoxyuridine ELISA assay and Ki67 gene expression was checked by real-time PCR. The migratory ability of endometrial stromal cells was determined by wound healing and transwell migration assays. Small RNA sequencing was applied on tissues collected from women with adenomyosis before and after bromocriptine treatment to identify differentially expressed microRNAs (miRNAs) after bromocriptine treatment. Bioinformatic methods were used for target gene prediction and the identification of biological pathways by enrichment procedures.

Results

Vaginal bromocriptine treatment reduced the Ki67 protein expression in the endometrium of women with adenomyosis and did not change the prolactin mRNA expression and protein concentration of prolactin in endometrial tissues. Bromocriptine significantly inhibited the proliferative and migrative abilities of endometrial stromal cells derived from women with adenomyosis in vitro. Moreover, small RNA sequencing revealed 27 differentially expressed miRNAs between the endometrium of women with adenomyosis before and after six months of vaginal bromocriptine treatment. KEGG pathway analysis on targeted genes of 27 miRNAs showed that several signaling pathways associated with cell proliferation and apoptosis were enriched after bromocriptine treatment.

Conclusion

Bromocriptine treatment exhibits an anti-proliferative effect in the endometrium of women with adenomyosis in vivo and in vitro. Bromocriptine might inhibit the proliferation of endometrial tissue in adenomyosis in part through the regulation of dysregulated microRNAs and proliferation-associated signaling pathways.
Clinical differences between small and large pheochromocytomas and paragangliomas
Authors: Zhao Lin , Li ZhiMao , Meng Xu , Fan Hua , Zhang ZengLei , Zhang ZhaoCai , Liu YeCheng , Zhou XianLiang , Zhu HuaDong
Published: March 2023

Background

Pheochromocytomas and paragangliomas (PPGLs) are neuroendocrine tumors, most of which are characterized by the release of catecholamine, and range in diameters from less than 1 cm to 10 cm or more. However, knowledge of the differences in clinical features between small and large PPGLs is insufficient.

Methods

A retrospective analysis of patients with PPGLs treated at our institution between January 2018 and June 2020 was performed. The clinical characteristics of patients were investigated, and comparisons were made between patients with large and small PPGLs. The logistic regression analysis was used to confirm the risk factors, and the receiver operating characteristic curve was used to evaluate the diagnostic performance of the variables.

Results

Totally 263 patients were included, including 110 patients in small tumor group and 153 patients in large tumor group. There were more male patients in the large tumor group (p=0.009). More patients had hypertension (p<0.001) and diabetes (p=0.002) in the large tumor group. The 24-h urinary epinephrine (24hU-E) (p < 0.001) and 24-h urinary norepinephrine (24hU-NE) (p=0.002) concentrations were higher in the large tumor group. In terms of tumor location, adrenal-PPGLs were more frequent in the large tumor group (p<0.001). Multivariate logistic regression analysis showed that male sex [odds ratio (OR): 2.871, 95% confidence interval (CI): 1.444–5.711, p=0.003], 24hU-E concentrations (OR: 1.025, 95% CI:1.004–1.047, p=0.020), 24hU-NE concentrations (OR: 1.002, 95%CI: 1.001–1.004, p=0.045), and adrenal-PPGLs (OR: 2.510, 95% CI:1.256–5.018, p=0.009) were positive risk factors for large tumors. Taking above variables into the same model, the area under the receiver operating characteristic curve of the model for predicting the large tumor was 0.772 (95% CI: 0.706–0.834). After the short-term follow-up, there was no significant difference in tumor recurrence between the two groups (p=0.681).

Conclusions

Significant differences in numerous clinical characteristics exist between large and small PPGLs. The male patients were more likely to be with large tumors, and such tumors were more likely to reside on the adrenal glands. Catecholamine measurements also help predict tumor size of PPGLs. Clinical decision-making will benefit from this information.
Association between HOMA-IR and ovarian sensitivity index in women with PCOS undergoing ART: A retrospective cohort study
Authors: Li Yan , Wang Yiwen , Liu Hai , Zhang Shaodi , Zhang Cuilian
Published: March 2023

Introduction

Insulin resistance (IR) may play a central role in the pathophysiology of polycystic ovary syndrome (PCOS). Controlled ovarian stimulation (COS) in PCOS women in the setting of assisted reproductive technology (ART) is always a challenge for clinicians. However, it remains unclear whether IR in women with PCOS correlates with reduced ovarian sensitivity to exogenous gonadotropin (Gn). This study aimed to explore the association between homeostasis model assessment of insulin resistance (HOMA-IR) and ovarian sensitivity index (OSI).

Methods

In this retrospective cohort study, we explored the association between Ln HOMA-IR and Ln OSI based on smoothing splines generated by generalized additive model (GAM). Then the correlation between HOMA-IR and OSI was further tested with a multivariable linear regression model and subgroup analysis.

Results

1508 women with PCOS aged 20-39 years undergoing their first oocyte retrieval cycle were included consecutively between 2018 until 2022. We observed a negative association between Ln HOMA-IR and Ln OSI by using smoothing splines. In multivariable linear regression analysis, the inverse association between Ln HOMA-IR and Ln OSI was still found in PCOS women after adjustment for potential confounders (β = -0.18, 95% CI -0.25, -0.11). Compared with patients with the lowest tertile of HOMA-IR, those who had the highest tertile of HOMA-IR had lower OSI values (β = -0.25, 95% CI -0.36, -0.15).

Discussion

Our study provided evidence for the inverse correlation between IR and the ovarian sensitivity during COS in PCOS women. Herein, we proposed new insights for individualized manipulation in PCOS patients with IR undergoing ART.
Quality evaluation of metabolic and bariatric surgical guidelines
Authors: Qin Zi-Han , Yang Xin , Zheng Ya-Qi , An Li-Ya , Yang Ting , Du Yu-Lu , Wang Xiao , Zhao Shu-Han , Li Hao-Han , Sun Cheng-Kai , Sun Da-Li , Lin Yue-Ying
Published: March 2023

Objective

To evaluate the quality of surgical guidelines on bariatric/metabolic surgery.

Methods

Four independent reviewers used the AGREE II (The Appraisal of Guidelines for Research and Evaluation II) tool to assess the methodological quality of the included guidelines and conducted a comparative analysis of the main recommendations for surgical methods of these guidelines.

Results

Nine surgical guidelines were included in this study. Five articles with AGREE II scores over 60% are worthy of clinical recommendation. The field of rigor of development was relatively low, with an average score of 50.82%. Among 15 key recommendations and the corresponding best evidence in the guidelines, only 4 key recommendations were grade A recommendations.

Conclusions

The quality of metabolic and bariatric guidelines is uneven, and there is much room for improvement.
Role of endocrine PACAP in age-related diseases
Authors: Toth Denes , Reglodi Dora , Schwieters Lili , Tamas Andrea
Published: March 2023

Pituitary adenylate cyclase activating polypeptide (PACAP) is a conserved neuropeptide, which confers diverse anti-aging endocrine and paracrine/autocrine effects, including anti-apoptotic, anti-inflammatory and antioxidant action. The results of the in vivo and in vitro experiments show that increasing emphasis is being placed on the diagnostic/prognostic biomarker potential of this neuropeptide in a wide array of age-related diseases. After the initial findings regarding the presence and alteration of PACAP in different body fluids in physiological processes, an increasing number of studies have focused on the changes of its levels in various pathological conditions associated with advanced aging. Until 2016 – when the results of previous human studies were reviewed – a vast majority of the studies had dealt with age-related neurological diseases, like cerebrovascular and neurodegenerative diseases, multiple sclerosis, as well as some other common diseases in elderly such as migraine, traumatic brain injury and post-traumatic stress disorder, chronic hepatitis and nephrotic syndrome. The aim of this review is to summarize the old and the new results and highlight those ‘classical’ and emerging clinical fields in which PACAP may become subject to further investigation as a diagnostic and/or prognostic biomarker in age-related diseases.
The concordance and discordance of diabetic kidney disease and retinopathy in patients with type 2 diabetes mellitus: A cross-sectional study of 26,809 patients from 5 primary hospitals in China
Authors: Liu Zhaoxiang , Li Xianglan , Wang Yanlei , Song Yanxia , Liu Qiang , Gong Junxia , Fan Wenshuang , Lv Chunmei , Cao Chenxiang , Zhao Wenhui , Xiao Jianzhong
Published: March 2023

Introduction

Diabetic kidney disease (DKD) and diabetic retinopathy (DR) share similar pathophysiological mechanisms. However, signs of DKD may be present at diagnosis of diabetes without retinopathy. Risk factors for the development of DKD and DR may not be identical.

Methods

This study aimed to evaluate the concordance and discordance between DKD and DR by investigating the distribution of DKD and DR in patients with type 2 diabetes mellitus from 5 Chinese cities. A total of 26,809 patients were involved in this study. The clinical characteristics were compared among patients based on the presence of DKD and DR. Logistic regression models were used to analyze the independent risk factors of DKD and DR.

Results

The prevalence of DKD and DR was 32.3% and 34.6%, respectively. Among eligible patients, 1,752 patients without DR had an increased urinary albumin-to-creatinine ratio (ACR) or reduced estimated glomerular filtration rate (eGFR), and 1,483 patients with DR had no DKD. The positive predictive value of DR for DKD was 47.4% and negative predictive value was 67.1%. Elder age, male gender, a longer duration of disease, higher values of waist circumference and HbA1c were associated with both DR and DKD. A lower educational level was associated with DR. Higher BP and TG would predict increased prevalence of DKD.

Conclusions

DKD and DR shared many risk factors, but a significant discordance was present in patients with type 2 diabetes mellitus. DKD was more strongly associated with blood pressure and triglycerides than DR.
Central and lateral neck involvement in papillary thyroid carcinoma patients with or without thyroid capsular invasion: A multi-center analysis
Authors: Yang Zheyu , Heng Yu , Zhou Jian , Tao Lei , Cai Wei
Published: March 2023

Purposes

To quantitatively predict the probability of cervical lymph node metastasis for papillary thyroid carcinomas (PTC) patients with or without thyroid capsular invasion (TCI), to guide the decision-making of management strategies for neck regions.

Methods

A total of 998 PTC patients from three medical centers were retrospectively analyzed.

Results

Patients with positive TCI (TCI group) exhibited higher risks for both CLNM and LLNM than those with negative TCI (no-TCI group). Patients receiving lateral lymph node dissection showed significantly higher incidence of relatively severe postoperative complications. For no-TCI group, factors including age less than 55 years old, male, the presence of bilateral disease and multifocality, and maximum tumor diameter (MTD)>=0.5cm were confirmed to be independent risk factors for CLNM, while the presence of bilateral disease and ipsilateral nodular goiter (iNG), and maximum positive CLN diameter (MCLND)>1.0cm independent factors for LLNM. Independent risk factors of LLNM for patients within the TCI group included MCLND>1.0cm, positive CLN number>=3, and the presence of iNG. Predictive models of CLNM and LLNM were established based on the aforementioned risk factors for patients within no-TCI and TCI groups. A meticulous and comprehensive risk stratification flow chart was established for a more accurate evaluation of central neck involvement including both CLNM and LLNM risk in PTC patients.

Conclusions

A meticulous and comprehensive stratification flow chart for PTC patients for quantitatively evaluating both CLNM and LLNM was constructed.
Solar-powered five-leg inverter-driven quasi-dynamic charging for a slow-moving vehicle
Authors: Shanmugam Yuvaraja , Narayanamoorthi R. , Vishnuram Pradeep , Savio Dominic , Yadav Arvind , Bajaj Mohit , Nauman Ali , Khurshaid Tahir , Kamel Salah
Published: March 2023

Road transport is becoming increasingly electric as it becomes more environmentally friendly. A green transportation system includes solar arrays along the roadside, encouraging the eco-friendly EV charging system. This paper proposes a road-side photovoltaic system to charge the batteries of slow-moving electric vehicles using a five-leg inverter. The five-legged inverter, which utilizes a stand-alone PV system to drive the charging pads, enhances the probability of achieving the sustainability goal. The limitations of the conventional H-bridge inverter, such as its more prominent design and higher number of switches or straightforward design and restricted power level, are addressed by this converter. The proposed 3.3 kW, 85 kHz inverter energizes the four transmitter pads while a receiver pad moves over the transmitter pads and inductively extracts the power. The d.c.-d.c. converter is used to feed the power to the proposed inverter. The P and O-based MPPT algorithm with a tuned PI controller is used to generate the driving pulses of the d.c.-d.c. converter. The signals are generated based on the voltage and current output of the solar panel output. This control algorithm ensures the stability of the system output response. Additionally, the tuned d.c.-d.c. converter achieves maximum efficiency independent of the load resistance. The system maintains constant power transfer profile concerning load resistance variations. The 520*520 mm Double D-pad transmits the power, while the series-series compensation network assists the charging pads in achieving resonance. The developed systems’ nominal charging voltage and current are 144 V, 20 A, with an equivalent battery resistance of 7.2 Ω.
Model emulators for the assessment of regional impacts and risks of climate change: A case study of rainfed maize production in Mexico
Authors: Estrada Francisco , Mendoza Alma , Murray Guillermo , Calderón-Bustamante Oscar , Botzen Wouter , De León Escobedo Teresa , Velasco Julián A.
Published: March 2023

The collection of publicly available databases about climate change and its impacts on natural and human systems is unprecedented and ever-growing. However, the requirements of information can vary widely among users depending on their region, socioenvironmental context, and interests. Moreover, in the current era of active mitigation and adaptation policies, information needs are frequently not satisfied even by these massive and variated collections of databases. The development and use of emulators can help closing this information gap by allowing users to approximate the output from complex models and create user-defined experiments, without being technically or computational demanding on the user. Here, a simple emulator of the EPIC biophysical crop model is presented which is able to adequately reproduce the changes in rainfed maize and to create projections for user-defined scenarios. Moreover, it allows to produce risk measures that are not available with the original model. The proposed methodology is illustrated with a case study of rainfed maize production in Mexico for a reference emissions scenario (SSP370) and two user-defined international mitigation policy scenarios. These scenarios represent 1) current international mitigation commitments and 2) a scenario in which China withdraws from international mitigation efforts. Results showed that, under the reference scenario, climate change could have widespread consequences on rainfed production all over the country with decreases in yields reaching up to 80% in the southeast and northeast of the country. These impacts can be partially modulated by the moderately ambitious mitigation commitments assumed in recent international agreements if all countries comply. However, a potential withdraw of China from these efforts would significantly reduce any benefits from international mitigation. Under all scenarios, changes in productivity impose increasing risks for already vulnerable populations and considerable economic costs at the state and national levels. These results suggest the urgent need for critical planning for adaptation in the agricultural sector of the country.
Diagnostic work-up and phenotypic characteristics of a family with variable severity of distal arthrogryposis type 2B (Sheldon-Hall syndrome) and TNNT3 pathogenic variant
Authors: Dabaj Ivana , Carlier Robert Y. , Dieterich Klaus , Desguerre Isabelle , Faure Julien , Romero Norma B. , Trang Wenting , Quijano-Roy Susana , Germain Dominique P.
Published: March 2023

Background: Sheldon–Hall syndrome (SHS) or distal arthrogryposis 2B (DA2B) is a rare clinically and genetically heterogeneous multiple congenital contracture syndrome characterized by contractures of the distal joints of the limbs and mild facial involvement, due to pathogenic variants in genes encoding the fast-twitch skeletal muscle contractile myofiber complex (TNNT3, TNNI2, TMP2, and MYH3 genes).

Patients and methods: A 16-year-old boy with a history of congenital distal arthrogryposis developed severe kyphoscoliosis and respiratory insufficiency. His mother and younger sister had phenotypes compatible with SHS but to a much lesser extent. Diagnostic work-up included physical examination and whole-body muscular MRI (WBMRI) in all three patients and electroneuromyography (ENMG) and paravertebral muscle biopsy in the proband. DNA sequencing was used to confirm the diagnosis.

Results: Physical examination suggested the diagnosis of SHS. No muscle signal abnormalities were found in WBMRI. Large motor unit potentials and reduced recruitment suggestive of neurogenic changes were observed on needle EMG in distal and paravertebral muscles in the proband. DNA sequencing revealed a pathogenic variant in TNNT3 (c.187C>T), which segregated as a dominant trait with the phenotype.

Discussion: This is the first report on neurogenic features in a patient with DA2B and a pathogenic variant in TNNT3 encoding the fast-twitch skeletal muscle contractile myofiber complex. A superimposed length-dependent motor nerve involvement was unexpected. Whether developmental disarrangements in number, distribution, or innervation of the motor unit in fetal life might lead to pseudo-neurogenic EMG features warrants further studies, as well as the role of genetic modifiers in SHS variability.
Coupled aquaponics: Optimizing hydraulic retention times using a parallel unit process water treatment approach
Authors: Tetreault Joseph , Fogle Rachel L. , Fogarty Sean , Guerdat Todd
Published: March 2023

Coupled aquaponics is the integration of recirculating aquaculture systems (RAS) and hydroponic cropping systems (HCS) into one system with shared culture water. Water conservation, revenue diversification, location-independent food production, and a reduced reliance on synthetically derived fertilizer salts have been identified as potential benefits of coupled aquaponic production. Despite these potential benefits, it has been difficult for producers to achieve success at the commercial scale. This review discusses how traditional linear coupled aquaponic system designs are not suited for intensive production due to a lack of scalability and water flow rate optimization to meet the differing requirements for finfish, vegetables, and water treatment with practices commensurate with the individual RAS and HCS industries. An alternative design for intensive coupled aquaponics is presented that utilizes a parallel unit process approach for independent hydraulic retention time optimization of each system component. The production benefits and scaling opportunities for each of the primary components in an aquaponics system are discussed. A review of recently published coupled aquaponics literature demonstrates that a system utilizing a parallel unit process design can result in more intensive fish production and a greater nutrient supply for plant production, leading to the potential for greater economic and space use efficiency.
Immunomodulation—a general review of the current state-of-the-art and new therapeutic strategies for targeting the immune system
Authors: Strzelec Martyna , Detka Jan , Mieszczak Patrycja , Sobocińska Małgorzata Katarzyna , Majka Marcin
Published: March 2023

In recent years, there has been a tremendous development of biotechnological, pharmacological, and medical techniques which can be implemented in the functional modulation of the immune system components. Immunomodulation has attracted much attention because it offers direct applications in both basic research and clinical therapy. Modulation of a non-adequate, amplified immune response enables to attenuate the clinical course of a disease and restore homeostasis. The potential targets to modulate immunity are as multiple as the components of the immune system, thus creating various possibilities for intervention. However, immunomodulation faces new challenges to design safer and more efficacious therapeutic compounds. This review offers a cross-sectional picture of the currently used and newest pharmacological interventions, genomic editing, and tools for regenerative medicine involving immunomodulation. We reviewed currently available experimental and clinical evidence to prove the efficiency, safety, and feasibility of immunomodulation in vitro and in vivo. We also reviewed the advantages and limitations of the described techniques. Despite its limitations, immunomodulation is considered as therapy itself or as an adjunct with promising results and developing potential.
B cell targeted therapies in inflammatory autoimmune disease of the central nervous system
Authors: Furman Moritz J. , Meuth Sven G. , Albrecht Philipp , Dietrich Michael , Blum Heike , Mares Jan , Milo Ron , Hartung Hans-Peter
Published: March 2023

Cumulative evidence along several lines indicates that B cells play an important role in the pathological course of multiple sclerosis (MS), neuromyelitisoptica spectrum disorders (NMOSD) and related CNS diseases. This has prompted extensive research in exploring the utility of targeting B cells to contain disease activity in these disorders. In this review, we first recapitulate the development of B cells from their origin in the bone marrow to their migration to the periphery, including the expression of therapy-relevant surface immunoglobulin isotypes. Not only the ability of B cells to produce cytokines and immunoglobulins seems to be essential in driving neuroinflammation, but also their regulatory functions strongly impact pathobiology. We then critically assess studies of B cell depleting therapies, including CD20 and CD19 targeting monoclonal antibodies, as well as the new class of B cell modulating substances, Bruton´s tyrosinekinase (BTK) inhibitors, in MS, NMOSD and MOGAD.
Integrating microbiome and metabolome revealed microbe-metabolism interactions in the stomach of patients with different severity of peptic ulcer disease
Authors: Wang Chao , Yu Xiao , Lin Hongqiang , Wang Guoqiang , Liu Jianming , Gao Chencheng , Qi Mingran , Wang Dan , Wang Fang
Published: March 2023

Background

Peptic ulcer disease (PUD) is a multi-cause illness with an unknown role for gastric flora and metabolism in its pathogenesis. In order to further understand the pathogenesis of gastric flora and metabolism in PUD, this study used histological techniques to analyze the microbiome and metabolome of gastric biopsy tissue. In this paper, our work described the complex interactions of phenotype-microbial-metabolite-metabolic pathways in PUD patients at different pathological stages.

Methods

Gastric biopsy tissue samples from 32 patients with chronic non-atrophic gastritis, 24 patients with mucosal erosions, and 8 patients with ulcers were collected for the microbiome. UPLC-MS metabolomics was also used to detect gastric tissue samples. These datasets were analyzed individually and integrated using various bioinformatics methods.

Results

Our work found reduced diversity of gastric flora in patients with PUD. PUD patients at different pathological stages presented their own unique flora, and there were significant differences in flora phenotypes. Coprococcus_2, Phenylobacterium, Candidatus_Hepatoplasma, and other bacteria were found in the flora of people with chronic non-atrophic gastritis (HC). The representative flora of mucosal erosion (ME) had uncultured_bacterium_c_Subgroup_6, Sphingomonadaceae, Xanthobacteraceae, and uncultured_bacterium_f_Xanthobacteraceae. In comparison, the characteristic flora of the PUD group was the most numerous and complex, including Ruminococcus_2, Agathobacter, Alistipes, Helicobacter, Bacteroides and Faecalibacterium. Metabolomics identified and annotated 66 differential metabolites and 12 significantly different metabolic pathways. The comprehensive analysis correlated microorganisms with metabolites at different pathological stages and initially explored the complex interactions of phenotype-microbial-metabolite-metabolic pathways in PUD patients at different pathological stages.

Conclusion

Our research results provided substantial evidence to support some data on the analysis of the microbial community and its metabolism in the stomach, and they demonstrated many specific interactions between the gastric microbiome and the metabolome. Our study can help reveal the pathogenesis of PUD and indicate plausible disease-specific mechanisms for future studies from a new perspective.
Emerging viral infections in immunocompromised patients: A great challenge to better define the role of immune response
Authors: Agrati Chiara , Bartolini Barbara , Bordoni Veronica , Locatelli Franco , Capobianchi Maria Rosaria , Di Caro Antonino , Castilletti Concetta , Ippolito Giuseppe
Published: March 2023

The immune response to invading pathogens is characterized by the rapid establishment of a complex network of cellular interactions and soluble signals. The correct balancing of activating and regulating pathways and tissue-homing signals determines its effectiveness and persistence over time. Emerging viral pathogens have always represented a great challenge to the immune system and an often uncontrolled/imbalanced immune response has been described (e.g. cytokine storm, immune paralysis), contributing to the severity of the disease. Several immune biomarkers and cell subsets have been identified as major players in the cascade of events leading to severe diseases, highlighting the rationale for host-directed intervention strategy. There are millions of immunocompromised pediatric and adult patients worldwide (e.g. transplant recipients, hematologic patients, subjects with primary immune-deficiencies), experiencing an impaired immune reactivity, due to diseases and/or to the medical treatments. The reduced immune reactivity could have two paradoxical non-exclusive effects: a weak protective immunity on one hand, and a reduced contribution to immune-mediated pathogenetic processes on the other hand. In these sensitive contexts, the impact of emerging infections represents a still open issue to be explored with several challenges for immunologists, virologists, physicians and epidemiologists. In this review, we will address emerging infections in immunocompromised hosts, to summarize the available data concerning the immune response profile, its influence on the clinical presentation, the possible contribution of persistent viral shedding in generating new viral variants with improved immune escape features, and the key role of vaccination.
Understanding gene regulation during the development of the sea cucumber Apostichopus japonicus using comparative transcriptomics
Authors: Su Fang , Liu Shilin , Xing Lili , Huo Da , Yang Hongsheng , Sun Lina
Published: March 2023

Embryonic development, especially metamorphosis and settlement, has a major impact on the life history of marine invertebrates. Apostichopus japonicus is an economically important species of sea cucumber. In this study, we performed RNA sequencing on six key stages of A. japonicas development: fertilized eggs, blastula, gastrula, auricularia, doliolaria, and pentactula. A total of 32,353 genes were identified and annotated as a reference gene set for subsequent pairwise comparison analysis. After filtering out low-quality genes, the dynamic molecular responses to development were revealed by WGCNA. The results showed that of the 20 modules, genes in the blue, yellow, and darkslateblue modules were highly correlated with the gastrula, auricularia, and blastula stages, respectively. GO terms for “RNA” and “proteasome complex” were most significantly enriched in the blue module. In the darkslateblue and yellow module, receptors of signaling pathways and metabolic processes were significantly enriched, respectively. All DEGs were categorized into 34 terms, mainly associated with signal transduction and cellular immunity. The expression pattern of genes associated with adhesion, cell cycle, signal, transcription factor, extracellular matrix (ECM), and cytoskeleton was analyzed according to gene function. The results of this study facilitated a more comprehensive understanding of the molecular characteristics of sea cucumber embryonic development and will provide theoretical guidance for larva rearing in sea cucumber culture.
The island rule with multiple islands and its application to the Indonesian Throughflow
Authors: Shi Wanli , Hu Shijian , Ma Hongji
Published: March 2023

The island rule theory in the case of complex geometry with multiple islands referring to the Indo-Pacific Maritime Continent is investigated on the basis of Godfrey’s island rule theory. The bottom friction and lateral friction of multiple channels are considered by employing the Munk and Stommel boundary layer models. Five idealized cases with various spatial distributions of islands are designed to examine the influence of shape and size of the islands. The analytical solutions of the streamfunctions of the through-flows among the islands are obtained and the volume transport through each channel is estimated. The Indonesian Throughflow (ITF) transport is then calculated using the analytical solutions with wind stress and compared with observations and previous theoretical results. The ITF transport from the multiple island rule is about 14.5 Sv during 2004–2006, which is close to the observed ITF transport (about 15.0 Sv) from the International Nusantara Stratification and Transport. We find that the multiple islands rule reproduces well the mean value and interannual variability of the observed ITF transport, and inclusion of wind stress in the North Pacific Ocean may improve the estimate of ITF transport. Sensitivity experiments indicate that frictional boundary layer thickness and channel size influence the estimated ITF transport under the multiple island framework. These results imply that the multiple island rule shows improvements in estimating the ITF transport relative to previous studies, and the multiple island rule can be used to produce long time series of ITF transport and might have implications for paleo-ITF study.
Utility and safety of airway stenting in airway stenosis after lung transplant: A systematic review
Authors: Wang Zilin , Zhao Bo , Deng Mingming , Tong Run , Bian Yiding , Zhang Qin , Hou Gang
Published: March 2023

Background

Airway complications seriously affect the clinical outcomes and long-term prognosis of lung-transplantation patients. Airway stenting provides effective palliation for patients with airway stenosis. However, a lack of consensus regarding the efficacy and safety of airway stents in airway stenosis after lung transplantation. This study critically evaluated all available evidence regarding this concern.

Methods

We retrieved studies from EMBASE, PubMed, and Cochrane Library databases. Studies were included if they reported baseline characteristics of airway complications after lung transplantation, stenting for airway stenosis, or prognosis.

Results

In total, 279 papers were screened and 17 papers were included in final analysis. The short-term efficacy of airway stenting was assessed in almost all studies, with immediate palliation in symptom and improved pulmonary function reported. Eleven of the included studies evaluated the long-term efficacy of stent therapy, with no distinct lung function. The median overall survival time was 1,124 (95% confidence interval 415–1,833) days in stented patients only. Stent-related complications are common regardless of the material; However, serious complications are rare and can be improved with routine management.

Conclusion

We demonstrated that airway stenting is a safe and effective method to treat airway stenosis after lung transplantation. The short-term effect was significant, while the long-term efficacy on survival rate needed further investigations.

Systematic review registration

www.crd.york.ac.uk/prospero/, identifier: CRD42022364427.
Sepsis and infection: Two words that should not be confused
Authors: Vincent Jean-Louis
Published: March 2023

The underlying cause of sepsis is a dysregulated host response to infection, leading to multiple organ failure. Identifying sepsis is crucial because of the associated pathophysiological, practical, and therapeutic implications, which will determine where and how the patient should be managed. In the absence of an end-of-life decision to limit therapies, the patient should be admitted to the intensive care unit immediately. Importantly, not all patients with sepsis are the same and being able to better characterize them is important. The future will focus on phenotypes to characterize critically ill patients, with or without infection, to enable more appropriate targeting of therapeutic interventions.
Natural fermentation quality, bacteria, and functional profiles of three cuttings of alfalfa silage in a year in Inner Mongolia, China
Authors: Sun Juanjuan , Wang Jing , Bai Chunsheng , Zhao Jinmei , Yun Ying , Yu Zhu , Xue Yanlin , Zhang Tengwei , Bao Wenlong
Published: March 2023

Alfalfa is harvested two or three times a year in central and western Inner Mongolia, China. However, the variations in bacterial communities as affected by wilting and ensiling, and the ensiling characteristics of alfalfa among the different cuttings, are not fully understood. To enable a more complete evaluation, alfalfa was harvested three times a year. At each time of cutting, alfalfa was harvested at early bloom, wilted for 6 h, and then ensiled in polyethylene bags for 60 days. The bacterial communities and nutritional components of fresh alfalfa(F), wilted alfalfa(W) and ensiled alfalfa(S), and the fermentation quality and functional profile of bacterial communities of the three cuttings alfalfa silage, were then analyzed. Functional characteristics of silage bacterial communities were evaluated according to the Kyoto Encyclopedia of Genes and Genomes. The results showed that all nutritional components, fermentation quality, bacterial communities, carbohydrate, amino acid metabolism and key enzymes of bacterial communities were influenced by cutting time. The species richness of F increased from the first cutting to the third cutting; it was not changed by wilting, but was decreased by ensiling. At phylum level, Proteobacteria were more predominant than other bacteria, followed by Firmicutes (0.063–21.39%) in F and W in the first and second cuttings. Firmicutes (96.66–99.79%) were more predominant than other bacteria, followed by Proteobacteria (0.13–3.19%) in S in the first and second cuttings. Proteobacteria, however, predominated over all other bacteria in F, W, or S in the third cutting. The third-cutting silage showed the highest levels of dry matter, pH and butyric acid (p < 0.05). Higher levels of pH and butyric acid were positively correlated with the most predominant genus in silage, and with Rosenbergiella and Pantoea. The third-cutting silage had the lowest fermentation quality as Proteobacteria were more predominant. This suggested that, compared with the first and second cutting, the third cutting is more likely to result in poorly preserved silage in the region studied.
New corticioid taxa in Phanerochaetaceae (Polyporales, Basidiomycota) from East Asia
Authors: Li Yue , Chen Che-Chih , He Shuang-Hui
Published: March 2023

The species diversity, taxonomy, and phylogeny of five corticioid genera of Phanerochaetaceae, namely, Hyphodermella, Roseograndinia, Phlebiopsis, Rhizochaete, and Phanerochaete, in East Asia are studied by using the morphological and molecular methods. Phylogenetic analyses were performed separately for the Donkia, Phlebiopsis, Rhizochaete, and Phanerochaete clades based on ITS1-5.8S-ITS2 and nrLSU sequence data. In total, seven new species were found, two new combinations are suggested, and a new name is proposed. In the Donkia clade, Hyphodermella sensu stricto was strongly supported with two new lineages, namely H. laevigata and H. tropica, which were recovered. Hyphodermella aurantiaca and H. zixishanensis are members of Roseograndinia, while R. jilinensis is proved to be a later synonym of H. aurantiaca. In the Phlebiopsis clade, P. cana sp. nov. was found on the bamboo from tropical Asia. In the Rhizochaete clade, four new species, R. nakasoneae, R. subradicata, R. terrestris, and R. yunnanensis were recovered based mainly on molecular analyses. In the Phanerochaete clade, P. subsanguinea nom. nov. is proposed to replace Phanerochaete rhizomorpha C.L. Zhao & D.Q. Wang, which is an invalid name because it was published after Phanerochaete rhizomorpha C.C. Chen, Sheng H. Wu & S.H. He, representing another species. Descriptions and illustrations are provided for the new species, and discussions are given for new taxa and names. Identification keys to Hyphodermella species worldwide and Rhizochaete species in China are given separately.
A scoring system based on novel biomarkers and clinical risk factors to predict invasive candidiasis in immunocompetent critically ill patients
Authors: Li Wen , Chen Gang , Lin Fengyu , Yang Hang , Cui Yanhui , Lu Rongli , Song Chao , Li Haitao , Li Yi , Pan Pinhua
Published: March 2023

Background

Delayed diagnosis further increases the mortality of invasive candidiasis (IC) in intensive care unit (ICU) patients. This study aimed to develop and validate a score based on novel serological biomarkers and clinical risk factors for predicting IC in immunocompetent ICU patients.

Methods

We retrospectively collected clinical data and novel serological markers on admission to ICU. Multivariate logistic regression was used to identify the risk factors associated with IC, which were adopted to establish a scoring system.

Results

Patients with IC had a higher C-reactive protein-to-albumin ratio (CAR) and neutrophil-to-lymphocyte ratio (NLR) and lower prognostic nutritional index than those without IC. The NLR, CAR, sepsis, total parenteral nutrition, 1,3-β-D-glucan (BDG)-positivity, and Sequential Organ Failure Assessment score were identified as independent risk factors for IC by multivariate logistic regression analysis and entered into the final scoring system. The area under receiver operating characteristic curve of the score were 0.883 and 0.892, respectively, in the development and validation cohort, higher than Candida score (0.883 vs.0.730, p < 0.001).

Conclusion

We established a parsimonious score based on NLR, CAR, BDG-positivity, and clinical risk factors, which can accurately identify IC in ICU patients to give treatment on time and reduce mortality.
Research Progress on the detection methods of porcine reproductive and respiratory syndrome virus
Authors: Pan Jinghua , Zeng Mengyi , Zhao Mengmeng , Huang Liangzong
Published: March 2023

Porcine reproductive and respiratory syndrome virus (PRRSV) causes clinical syndromes typified as reproductive disorders in sows and respiratory diseases in piglets. PRRSV remains one of the most prevalent pathogens affecting the pig industry, because of its complex infection profile and highly heterogeneous genetic and recombination characteristics. Therefore, a rapid and effective PRRSV detection method is important for the prevention and control of PRRS. With extensive in-depth research on PRRSV detection methods, many detection methods have been improved and promoted. Laboratory methods include techniques based on virus isolation (VI), enzyme-linked immunosorbent assays (ELISA), indirect immunofluorescence assays (IFA), immunoperoxidase monolayer assays (IPMA), polymerase chain reaction (PCR), quantitative real-time PCR (qPCR), digital PCR (dPCR), loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA), clustered regularly interspaced short palindromic repeats (CRISPR), metagenomic next-generation sequencing (mNGS), and other methods. This study reviews the latest research on improving the main PRRSV detection methods and discusses their advantages and disadvantages.
Emerging trends and focus on the link between gut microbiota and type 1 diabetes: A bibliometric and visualization analysis
Authors: Guo Keyu , Li Jiaqi , Li Xia , Huang Juan , Zhou Zhiguang
Published: March 2023

Objective

To conduct the first thorough bibliometric analysis to evaluate and quantify global research regarding to the gut microbiota and type 1 diabetes (T1D).

Methods

A literature search for research studies on gut microbiota and T1D was conducted using the Web of Science Core Collection (WoSCC) database on 24 September 2022. VOSviewer software and the packages Bibliometrix R and ggplot used in RStudio were applied to perform the bibliometric and visualization analysis.

Results

A total of 639 publications was extracted using the terms “gut microbiota” and “type 1 diabetes” (and their synonyms in MeSH). Ultimately, 324 articles were included in the bibliometric analysis. The United States and European countries are the main contributors to this field, and the top 10 most influential institutions are all based in the United States, Finland and Denmark. The three most influential researchers in this field are Li Wen, Jorma Ilonen and Mikael Knip. Historical direct citation analysis showed the evolution of the most cited papers in the field of T1D and gut microbiota. Clustering analysis defined seven clusters, covering the current main topics in both basic and clinical research on T1D and gut microbiota. The most commonly found high-frequency keywords in the period from 2018 to 2021 were “metagenomics,” “neutrophils” and “machine learning.”

Conclusion

The application of multi-omics and machine learning approaches will be a necessary future step for better understanding gut microbiota in T1D. Finally, the future outlook for customized therapy toward reshaping gut microbiota of T1D patients remains promising.