bluuebunny/crossref_metadata_2025 huggingface dataset:
Synergistic growth inhibition by acyclic retinoid and phosphatidylinositol 3-kinase inhibitor in human hepatoma cells
Authors: Baba Atsushi , Shimizu Masahito , Ohno Tomohiko , Shirakami Yohei , Kubota Masaya , Kochi Takahiro , Terakura Daishi , Tsurumi Hisashi , Moriwaki Hisataka
Published: October 2013

Background

A malfunction of RXRα due to phosphorylation is associated with liver carcinogenesis, and acyclic retinoid (ACR), which targets RXRα, can prevent the development of hepatocellular carcinoma (HCC). Activation of PI3K/Akt signaling plays a critical role in the proliferation and survival of HCC cells. The present study examined the possible combined effects of ACR and LY294002, a PI3K inhibitor, on the growth of human HCC cells.

Methods

This study examined the effects of the combination of ACR plus LY294002 on the growth of HLF human HCC cells.

Results

ACR and LY294002 preferentially inhibited the growth of HLF cells in comparison with Hc normal hepatocytes. The combination of 1 μM ACR and 5 μM LY294002, in which the concentrations used are less than the IC50values of these agents, synergistically inhibited the growth of HLF, Hep3B, and Huh7 human HCC cells. These agents when administered in combination acted cooperatively to induce apoptosis in HLF cells. The phosphorylation of RXRα, Akt, and ERK proteins in HLF cells were markedly inhibited by treatment with ACR plus LY294002. Moreover, this combination also increased RXRE promoter activity and the cellular levels of RARβ and p21CIP1, while decreasing the levels of cyclin D1.

Conclusion

ACR and LY294002 cooperatively increase the expression of RARβ, while inhibiting the phosphorylation of RXRα, and that these effects are associated with the induction of apoptosis and the inhibition of cell growth in human HCC cells. This combination might therefore be effective for the chemoprevention and chemotherapy of HCC.
Ethnicity and suicide attempt: analysis in bipolar disorder and schizophrenia
Authors: Bani-Fatemi Ali , Polsinelli Gina , Kennedy James L , De Luca Vincenzo
Published: October 2013

Background

Evidence is mixed as to whether White Europeans are at a higher risk for suicide attempts or completions compared to other ethnic groups. The present analysis assessed whether risk for suicide attempt was associated with White European ethnicity in 907 subjects with schizophrenia or bipolar disorder.

Methods

Subjects were diagnosed using the Structured Clinical Interview for DSM-IV, and ethnicity was determined by self-report. Subjects were recruited from psychiatric care centers in Toronto, Canada. Logistic regression correcting for clinical covariates like age, gender and diagnosis, was used in this study.

Results

We found no difference in suicide attempter status in white and non-white subjects who were diagnosed with schizophrenia and bipolar disorder.

Conclusion

Our study does not support the evidence that White-European patients in North America are at higher risk for suicide attempt compared to non-European descent subjects. However, this result has to be replicated in larger studies in patients with these disorders.
Adrenomedullin delivery in microsphere-scaffold composite for remodeling of the alveolar bone following tooth extraction: an experimental study in the rat
Authors: Wang Lin , Zheng Ling , Li Chunyan , Dong Shujun , A Lan , Zhou Yanmin
Published: October 2013

Background

Alveolar ridge resorption, as a significant problem in implant and restorative dentistry, has long been considered as an inevitable outcome following tooth extraction. Recently, adrenomedullin (ADM) is reported to be able to stimulate the proliferation and migration of various cells including osteoblasts. The purpose of this study was to investigate the influence of local ADM application in the tooth extraction socket in vivo.

Methods

Chitosan micropheres were developed by an emulsion-ionic cross-linking method for ADM delivery. Poly (L -lactic-co-glycolic) acid (PLGA) and nano-hydroxyapatite (nHA) were used to prepare scaffolds to contain the micrspheres with ADM. In vivo experiment was evaluated by transplanting the composite into the rat socket right after the incisor extraction. After 4, 8, 12 weeks implantation, radiographic and histological tests were carried out to evaluate the effect of released ADM on the alveolar bone.

Results

The microspheres had a spherical structure and a relative rough and uniform surface, and the particle size was under a normal distribution, with the average diameter of 38.59 μm. The scaffolds had open and interconnected pores. In addition, the high porosity of the composite was 88.93%. Radiographic and histological examination revealed that the PLGA/nHA/CMs/ADM composite could accelerate the alveolar bone remodeling and reduce the residual ridge resorption compared with the PLGA/nHA/CMs scaffold.

Conclusions

The results of this study suggest that local application of ADM has the potential to preserve the residual alveolar ridge and accelerate the alveolar bone remodeling.
Effects of exercise and diet change on cognition function and synaptic plasticity in high fat diet induced obese rats
Authors: Woo Jinhee , Shin Ki Ok , Park So Young , Jang Ki Soeng , Kang Sunghwun
Published: October 2013

Background

Nutritional imbalance-induced obesity causes a variety of diseases and in particular is an important cause of cognitive function decline. This study was performed on Sprague Dawley (SD) rats with 13-weeks of high fat diet-induced obesity in connection to the effects of regular exercise and dietary control for 8 weeks on the synaptic plasticity and cognitive abilities of brain.

Methods

Four weeks-old SD rats were adopted classified into normal-normal diet-sedentary (NNS, n = 8), obesity-high fat diet-sedentary (OHS, n = 8), obesity-high fat diet-training (OHT, n = 8), obesity-normal diet-sedentary (ONS, n = 8) and obesity- normal diet-training (ONT, n = 8). The exercise program consisted of a treadmill exercise administered at a speed of 8 m/min for 1–4 weeks, and 14 m/min for 5–8 weeks. The Western blot method was used to measure the expression of NGF, BDNF, p38MAPK and p-p38MAPK proteins in hippocampus of the brain, and expressions of NGF, BDNF, TrkA, TrkB, CREB and synapsin1 mRNA were analyzed through qRT-PCR.

Results

The results suggest cognitive function-related protein levels and mRNA expression to be significantly decreased in the hippocampus of obese rats, and synaptic plasticity as well as cognitive function signaling sub-pathway factors were also significantly decreased. In addition, 8-weeks exercises and treatment by dietary change had induced significant increase of cognitive function-related protein levels and mRNA expression as well as synaptic plasticity and cognitive function signaling sub-pathway factors in obese rats. In particular, the combined treatment had presented even more positive effect.

Conclusions

Therefore, it was determined that the high fat diet-induced obesity decreases plasticity and cognitive function of the brain, but was identified as being improved by exercises and dietary changes. In particular, it is considered that regular exercise has positive effects on memory span and learning capacity unlike dietary control.
Anti-CEA-functionalized superparamagnetic iron oxide nanoparticles for examining colorectal tumors in vivo
Authors: Huang Kai-Wen , Chieh Jen-Jie , Lin In-Tsang , Horng Herng-Er , Yang Hong-Chang , Hong Chin-Yih
Published: October 2013

Although the biomarker carcinoembryonic antigen (CEA) is expressed in colorectal tumors, the utility of an anti-CEA-functionalized image medium is powerful for in vivo positioning of colorectal tumors. With a risk of superparamagnetic iron oxide nanoparticles (SPIONPs) that is lower for animals than other material carriers, anti-CEA-functionalized SPIONPs were synthesized in this study for labeling colorectal tumors by conducting different preoperatively and intraoperatively in vivo examinations. In magnetic resonance imaging (MRI), the image variation of colorectal tumors reached the maximum at approximately 24 h. However, because MRI requires a nonmetal environment, it was limited to preoperative imaging. With the potentiality of in vivo screening and intraoperative positioning during surgery, the scanning superconducting-quantum-interference-device biosusceptometry (SSB) was adopted, showing the favorable agreement of time-varied intensity with MRI. Furthermore, biological methodologies of different tissue staining methods and inductively coupled plasma (ICP) yielded consistent results, proving that the obtained in vivo results occurred because of targeted anti-CEA SPIONPs. This indicates that developed anti-CEA SPIONPs owe the utilities as an image medium of these in vivo methodologies.
Qualitative properties of a cooperative degenerate Lotka-Volterra model
Authors: Sun Jiebao , Zhang Dazhi , Wu Boying
Published: October 2013

This paper considers a kind of degenerate parabolic systems. First, we consider the initial boundary value problem of the two-species degenerate parabolic cooperative system. By using the method of a parabolic regularization and energy estimate, we establish the existence of the weak solution of the problem. Then we establish the comparison principle and discuss the uniqueness and the uniform bound. At last, we consider the periodic boundary value problem of the system. By constructing a pair of ordered upper and lower solutions, we establish the existence of nontrivial nonnegative periodic solutions.
Mosaic pregnancy after transfer of a “euploid” blastocyst screened by DNA microarray
Authors: Haddad Ghassan , He Wenyin , Gill Jimmy , Witz Craig , Wang Cassie , Kaskar Khalied , Wang Weihua
Published: October 2013

Background

High proportions of human embryos produced by in vitro fertilization are aneuploidy and mosaic. DNA microarray is one of the most practical screening methods to select euploid embryos for transfer. However, mosaic pregnancy is still possible due to embryonic mosacism. Here we report a successful pregnancy after transfer of a mosaic blastocyst with euploid inner cell mass.

Methods

A woman with a previous trisomy 13 pregnancy pursued infertility treatment with preimplantation genetic screening by a trophectoderm biopsy and DNA microarray. NimbleGen oligonucleotide DNA microarray was applied to biopsied samples from 13 blastocysts. A euploid blastocyst was transferred to the patient and subsequent prenatal cytogenetic tests were performed by FISH and/or G banding.

Results

Following DNA microarray, it was found that 5 blastocysts were euploid and 8 were aneuploidy. Transfer of one euploid blastocyst resulted in a clinical pregnancy. Prenatal cytogenetic tests of samples biopsied from chorionic villi sample showed both trisomy 21 (47 XX, +21) and euploid (46, XX) cells. Further prenatal cytogenetic test with a sample from amniotic fluid indicated that all cells were euploid (46, XX). The pregnancy was continued and a healthy girl was delivered after 41 weeks of gestation.

Conclusions

This is the first report to indicate a mosaic pregnancy after transfer of a “euploid” blastocyst that was screened by DNA microarray, and the case further confirms that mosaicism is present in human blastocysts produced by in vitro fertilization.
Impact of fibrinogen concentrate alone or with prothrombin complex concentrate (+/− fresh frozen plasma) on plasma fibrinogen level and fibrin-based clot strength (FIBTEM) in major trauma: a retrospective study
Authors: Schlimp Christoph J , Voelckel Wolfgang , Inaba Kenji , Maegele Marc , Schöchl Herbert
Published: October 2013

Background

Low plasma fibrinogen concentration is a predictor of poor outcome in major trauma patients. The role of fibrinogen concentrate for rapidly increasing fibrinogen plasma levels in severe trauma is not well defined.

Methods

In this retrospective study we included severe trauma patients treated with fibrinogen concentrate alone (FC group), fibrinogen concentrate with prothrombin complex concentrate (FC–PCC group) or fibrinogen concentrate with PCC and fresh frozen plasma (FC–PCC–FFP group). PCC was generally administered as the second step of intraoperative therapy, while FFP was only administered as a third step. All patients received ≥1 g fibrinogen concentrate within 24 hours. Plasma fibrinogen concentration and ROTEM parameters upon emergency room (ER) admission, intensive care unit (ICU) admission, and after 24 hours were analysed.

Results

Among 157 patients fulfilling the inclusion criteria, 83% were male; mean age was 44 years and median injury severity score (ISS) was 29. Standard coagulation tests reflected increasing severity of coagulopathy with increasing complexity of haemostatic therapy (highest severity in the FC–PCC–FFP group; p < 0.0001). Total 24-hour fibrinogen concentrate dose also increased with complexity of haemostatic therapy. Plasma fibrinogen concentration was maintained, with no significant difference between ER admission and ICU admission in all patient groups. FIBTEM clot firmness at 10 minutes (CA10) was similarly maintained, albeit with a small increase in the FC–PCC group. Fibrinogen concentration and FIBTEM CA10 were within the normal range in all groups at 24 hours. The ratio of fibrinogen concentrate to red blood cells (g:U) ranged between 0.7:1.0 and 1.0:1.0.

Conclusion

Fibrinogen concentrate therapy maintained fibrinogen concentration and FIBTEM CA10 during the initial phase of trauma care until ICU admission. After 24 hours, these parameters were comparable between the three groups and within the normal range for each of them. Further studies are warranted to investigate the effect of fibrinogen concentrate on clinical outcomes.
High-resolution 2D seismic imaging and forward modeling of a polymetallic sulfide deposit at Garpenberg, central Sweden
Authors: Ahmadi Omid , Juhlin Christopher , Malehmir Alireza , Munck Mie
Published: October 2013

We acquired a high-resolution 2D seismic profile to test the capability of the seismic method in imaging a sulfide ore body at Garpenberg, central Sweden. Delineation of the geologic structures, which surround and host the ore body, is another goal of the survey. Due to the 3D geology of the structures, a cross-dip correction performed to image out-of-the-plane reflections, resulting in a clear high amplitude anomaly at a time and location to that to be expected from near the top of the ore body. Furthermore, DMO processing and migration are applied to the data, providing images of four main reflection groups. The reflections have been interpreted as corresponding to geologic rock units in the area that partly interfere with the potential ore body signal. To further investigate the seismic response of the ore body, forward modeling by ray-tracing is applied using the ore body geometry as mapped by drilling. We use two ray-tracing approaches: standard 3D ray-tracing and an exploding reflector approach. Seven representative samples from the mine area are used to determine P-wave velocities. The measurements show a considerable contrast between the ore body and host rock. By comparing the modeled and observed data, we find that the high amplitude signal in the real seismic section most likely emanates from near the top of one concentrated ore which lies inside the larger mapped ore body that has been modeled as a resource. The base of the ore body is only observed on the synthetic data whereas a signal penetration analysis suggests that the seismic signal penetrated efficiently along the entire survey line. Presence of disseminated ore and lower fold toward the northern end of the profile could be combined reasons that make imaging the base of the ore body difficult.
NCX3 regulates mitochondrial calcium handling through AKAP121-anchored signaling complex and prevents hypoxia-induced cell death
Authors: Scorziello Antonella , Savoia Claudia , Sisalli Maria Josè , Adornetto Annagrazia , Secondo Agnese , Boscia Francesca , Esposito Alba , Polishchuk Elena V. , Polishchuk Roman S. , Molinaro Pasquale , Carlucci Annalisa , Lignitto Luca , Di Renzo Gianfranco , Feliciello Antonio , Annunziato Lucio
Published: October 2013

The mitochondrial influx and efflux calcium pathways play a relevant role in cytosolic and mitochondrial calcium homeostasis and contribute to the regulation of mitochondrial functions in neurons. The mitochondrial Na+/Ca2+ exchanger, although hypothesized in 1974, has been primarily investigated only from a functional point of view and its identity and localization in the mitochondria have been a matter of debate over the last three decades. Recently, a lithium-dependent sodium/calcium exchanger extruding calcium from the matrix has been found in the inner mitochondrial membrane of neuronal cells. However, evidence has been provided that the outer membrane is impermeable to calcium efflux into the cytoplasm.

In this study, we have demonstrated for the first time that the nuclear encoded NCX3 isoform (a) is localized on the outer mitochondrial membrane (OMM) of neurons, (b) co-localizes and immunoprecipitates with AKAP121, a member of the protein kinase A anchoring proteins (AKAPs) present on the outer membrane, (c) extrudes calcium from mitochondria through AKAP121 interaction in a PKA-mediated manner, both under normoxia and hypoxia, and (d) improves cell survival when it works in the Ca2+ efflux mode at the level of the OMM.

Collectively, these results suggest that, in neurons, NCX3 regulates mitochondrial calcium handling from the OMM through an AKAP121-anchored signalling complex, thus promoting cell survival during hypoxia.
Regulation of endoplasmic reticulum Ca2+ oscillations in mammalian eggs
Authors: Wakai Takuya , Zhang Nan , Vangheluwe Peter , Fissore Rafael A.
Published: October 2013

Changes in the intracellular concentration of free calcium ([Ca2+]i) regulate diverse cellular processes including fertilization. In mammalian eggs, the [Ca2+]i changes induced by the sperm unfolds in a pattern of periodical rises, also known as [Ca2+]i oscillations. The source of Ca2+ during oscillations is the endoplasmic reticulum ([Ca2+]ER), but is presently unknown how [Ca2+]ER is regulated. Here we show using mouse eggs that [Ca2+]i oscillations induced by a variety of agonists, including PLCζ, SrCl2 and thimerosal, provoked simultaneous but opposite changes in [Ca2+]ER and caused differential effects on the refilling and overall load of [Ca2+]ER. We also found that Ca2+ influx is required to refill [Ca2+]ER, as in media devoid of Ca2+, the loss of [Ca2+]ER was accelerated. Pharmacological inactivation of the function of the mitochondria and of the Ca2+-ATPase pumps PMCA and SERCA altered the pattern of oscillations and abruptly reduced [Ca2+]ER, especially after inactivation of mitochondria and SERCA functions. We also examined the expression of SERCA2b protein and found it expressed throughout oocyte maturation and attaining a conspicuous cortical cluster organization in mature eggs. We showed that its overexpression reduces the duration of IP3-induced [Ca2+]i rises, promoted initiation of oscillations and enhanced refilling of [Ca2+]ER. Collectively, our results provide novel insights on the regulation of [Ca2+]ER oscillations, which underlie the unique Ca2+ signalling system that activates the developmental program in mammalian eggs.
Preparation and Characterization of Oligo(9,9′-di-p-tolyl-3,3′-bicarbazyl) and Its Application to Transparent Conducting Materials
Authors: Kim Youngwoong , Tokuda Takuya , Yagishita Fumitoshi , Masu Hyuma , Kubo Minako , Murashiro Katsuyuki , Hoshino Katsuyoshi
Published: October 2013

The oxidation of 9-p-tolylcarbazole by iron(III) perchlorate in acetonitrile produces oligo(9,9′-di-p-tolyl-3,3′-bicarbazyl), partially doped with perchlorate. To the chloroform solution of the oligomers, we added metals to produce hybrid solutions. The hybrid films, prepared by applying the solutions onto a glass plate, exhibited good transparencies (90% or above in the visible region) and electrical conductivities of 4.1 × 10−3 S cm−1.
Stimuli-responsive Unimer Nanoparticles Composed of Poly(amino acid) Derivatives as Promising Protein-mimetic Drug Carriers
Authors: Piyapakorn Phassamon , Akagi Takami , Akashi Mitsuru
Published: October 2013

Stimuli-responsive unimer nanoparticles (NPs) composed of hydrophobized poly(γ-glutamic acid) were successfully prepared by a combination of hydrophobic interactions and disulfide bonds. The formation of hydrophobic associations and the existence of disulfide bonds were confirmed by UV spectroscopy and by treatment with dithiothreitol, respectively. Moreover, the release of hydrophobic drugs in response to a reducing agent was observed when compared to nonstimulus unimer NPs. These 10 nm-sized unimer NPs will be useful as reduction-responsive carriers.
Unique Synthetic Approach toward a Phosphaalkene: Synthesis of a Selenium-substituted Phosphaalkene with Bulky Substituents
Authors: Sugamata Koh , Sasamori Takahiro , Tokitoh Norihiro
Published: October 2013

A selenium-substituted phosphaalkene, (Me3Si)2C=PSeTrp (1, Trp: 9-triptycyl), was synthesized by two methods, substitution reaction of (Me3Si)2C=PCl (3) with TrpSeLi, and treatment of TsiP(Cl)SeTrp (4, Tsi: C(SiMe3)3) with a catalytic amount of AlCl3. The newly obtained phosphaalkene was characterized in detail.
On Portfolio Choice with Savoring and Disappointment
Authors: Jouini Elyès , Karehnke Paul , Napp Clotilde
Published: October 2013

We revisit the model proposed by Gollier and Muermann [Gollier C, Muermann A (2010) Optimal choice and beliefs with ex ante savoring and ex post disappointment. Management Sci. 56(8):1272–1284; hereafter, GM]. In the GM model, for a given lottery, agents form anticipated expected payoffs and the set of possible anticipations is assumed to be exogenously fixed. We propose sets of possible anticipations that are endogenously determined. This permits us to compare and evaluate in a consistent manner lotteries with different supports and to revisit the portfolio choice problem. We obtain new conclusions and interesting insights. Our extended model can rationalize a variety of empirically observed puzzles such as a positive demand for assets with negative expected returns, preference for skewed returns, and underdiversification of portfolios.

This paper was accepted by Rakesh Sarin, decision analysis.
Normal isometric strength of rotator cuff muscles in adults
Authors: Chezar A. , Berkovitch Y. , Haddad M. , Keren Y. , Soudry M. , Rosenberg N.
Published: October 2013

Objectives

The most prevalent disorders of the shoulder are related to the muscles of rotator cuff. In order to develop a mechanical method for the evaluation of the rotator cuff muscles, we created a database of isometric force generation by the rotator cuff muscles in normal adult population. We hypothesised the existence of variations according to age, gender and dominancy of limb.

Methods

A total of 400 healthy adult volunteers were tested, classified into groups of 50 men and women for each decade of life. Maximal isometric force was measured at standardised positions for supraspinatus, infraspinatus and subscapularis muscles in both shoulders in every person. Torque of the force was calculated and normalised to lean body mass. The profiles of mean torque-time curves for each age and gender group were compared.

Results

Our data showed that men gradually gained maximal strength in the fifth decade, and showed decreased strength in the sixth. In women the maximal strength was gained in the fourth decade with gradual decline to the sixth decade of life. The dominant arm was stronger in most of the tested groups. The torque profiles of the rotator cuff muscles in men at all ages were significantly higher than that in women.

Conclusions

We found previously unrecognised variations of rotator cuff muscles’ isometric strength according to age, gender and dominancy in a normal population. The presented data may serve as a basis for the future studies for identification of the abnormal patterns of muscle isometric strength in patients with pathology of the rotator cuff muscles. Cite this article: Bone Joint Res 2013;2:214–19.
Cardiovascular Disease Risk Prediction in Women: Is There a Role for Novel Biomarkers?
Authors: Paynter Nina P , Everett Brendan M , Cook Nancy R
Published: October 2013

BACKGROUND

Risk prediction is an integral part of the current US guidelines for cardiovascular disease in women. Although current risk prediction algorithms exist to identify women at increased 10-year risk of cardiovascular disease (CVD), clinicians and researchers have been interested in developing novel biomarkers that might improve predictive accuracy further. These biomarkers have led to important insights into the pathophysiology of CVD, but results for their ability to improve prediction or guide preventive therapy have been mixed. The incidence of CVD is lower in women than men, and the effects of a number of traditional biomarkers on CVD risk differ in women compared to men. Both of these factors influence the ability to accurately predict CVD risk.

CONTENT

We review the distinctive aspects of CVD risk prediction in women, discuss the statistical challenges to improved risk prediction, and discuss a number of biomarkers in varying stages of development with a range of performance in prediction.

A variety of biomarkers from different pathophysiologic pathways have been evaluated for improving CVD risk. While many have been incompletely studied or have not been shown to improve risk prediction in women, others, such as high-sensitivity troponin T, have shown promise in improving risk prediction. Increasing inclusion of women in CVD studies will be crucial to providing opportunities to evaluate future biomarkers.
Practices of un-disciplining: Notes on the interface of dance and moving image in performance
Authors: Harrington Chrissie , Sharma Aparna
Published: October 2013

This article articulates a series of creative practices in which ‘un-disciplining’ becomes central to making inter-disciplinary Dance-Moving Image (Dance-MI) performance. In this context Dance-MI performance implies the discourses between the live body and moving image within a performance whole. Motivating questions are: what sorts of interface processes could be devised and what would happen as a result? The integration of theory and practice is central to our interrogations and inspires movement, MI and sound materials. The article dwells on the processes by which movement and MI materials are generated and placed in dialogue with each other, and the complex potentials of those relationships. Practices recognize and build upon the specificities, overlaps and disparities between both Dance and MI, catapulting each form out of its determined disciplinary confine into spaces where sharing, exposing and recreating inspire new choreographies and thus new experiences for maker, performer and viewer. We articulate stilling, connecting and sensing as key concepts that emerge from our practice and are exemplified in selected works created by and with undergraduate students. The un-disciplining of Dance and MI as two movement-based forms liberate each to interface with the other and so create a new wholeness of experience that in turn privileges multiple perspectives over any singular vantage point. The article concludes by positing Dance-MI performance as experiential through a newness created by the co-presence and interaction between both forms – dance and moving image.
Reinventing the past: Rosemary Butcher encounters Allan Kaprow’s 18 Happenings in 6 Parts
Authors: Sachsenmaier Stefanie
Published: October 2013

This article discusses British choreographer Rosemary Butcher’s reinvention process in the context of Allan Kaprow’s 18 Happenings in 6 Parts. It argues that the connection to Kaprow’s first Happening from 1959 was meticulously processed by Butcher on a conceptual level, and that her 2010 reinvention, commissioned and presented at London’s Royal Festival Hall as part of ‘Move: Choreographing You’, was conceptually much closer aligned to Kaprow’s Happening than the actual live work might have suggested. Within this context the article enters established debates around choreographic adaptations and raises questions related to the ways archived performance work might be processed in order to produce ‘new’ signature practice, in Susan Melrose’s terms. It adopts Jean François Lyotard’s notion of the ‘dispositif’, or set-up, in order to explicate Butcher’s reinvention process.
Retos para alcanzar la excelencia en comunicación corporativa en los clubs deportivos profesionales españoles
Authors: Sanahuja Peris Guillermo , Blay Arráez Rocío
Published: October 2013

La presente investigación trata de presentar los principales retos que deben afrontar los clubes deportivos profesionales españoles para alcanzar la excelencia –entendida como el reconocimiento positivo por parte de los distintos públicos y grupos de interés. La metodología empleada es una combinación de técnicas cuantitativas y cualitativas ante un universo de estudio formado por las 56 entidades deportivas que forman la Liga de Fútbol Profesional (LFP) y la Asociación de Clubes de Baloncesto (ACB). Como resultado se señalan cinco palancas de cambio orientadas hacia ese horizonte de actuación excelente.
La audiencia de la ciberprensa española en los mercados de habla hispana
Authors: Cea Esteruelas Mª Nereida
Published: October 2013

El artículo analiza, en primer lugar, la audiencia de la ciberprensa española, lo que permite constatar que una parte importante de los usuarios proceden de Hispanoamérica. A continuación, el análisis de los ciberperiódicos más seguidos en los principales países de Hispanoamérica muestra la importante cuota de mercado de los medios españoles. En Argentina, Colombia, México y Venezuela, elmundo.es y elpais.com aparecen entre los cuatro ciberperiódicos con mayor número de usuarios. Por último, el estudio de la edición del diario digital español elmundo.es para Hispanoamérica recoge los aspectos fundamentales de la estrategia de contenidos seguida por este medio, que se ha consolidado líder en el mercado de habla hispana.
Radiografía de la Universidad de Alicante a través de las noticias emitidas en el Diario Información
Authors: Rodríguez-Monteagudo Eliseo , Hidalgo-Marí Tatiana
Published: October 2013

La constante actividad docente, cultural, investigadora y empresarial de la Universidad de Alicante genera un gran volumen informativo de alto interés periodístico, parte del cual se difunde a través de los medios de comunicación. En este sentido el Diario Información, como periódico impreso de mayor tirada de la provincia de Alicante, no sólo recoge y publica todos estos sucesos noticiosos sino que también permite que la actividad docente e investigadora pueda divulgarse abiertamente en sus páginas. El contenido resultante, por su formato y temática, además de informar sobre la labor multidisciplinar de la Universidad de Alicante, proyecta, voluntaria o involuntariamente, una imagen de dicha organización que puede condicionar la reputación de ésta en el ámbito social, político, económico y cultural. Por tanto, este flujo informativo es capaz de influir en la opinión pública así como en el valor intangible de la marca de la propia Universidad. El presente artículo trata de analizar el contenido que el Diario Información difunde acerca de Universidad de Alicante para poder determinar sus particularidades y averiguar si estos enunciados contribuyen a la generación de una imagen positiva de la Institución. Asimismo pretende constatar la capacidad de la UA para generar contenido periodístico de interés notable tanto para el diario en cuestión como para los públicos receptores del mismo.
Innovation in Tow: R&D FDI and investment incentives
Authors: Wellhausen Rachel L.
Published: October 2013

Multinational corporations (MNCs) increasingly internationalize research and development (R&D), but the distribution of foreign direct investment (FDI) in R&D differs from that of general FDI. I use data on US MNC affiliates’ investments abroad (2001–2008) to demonstrate that increasing value added predicts more future R&D FDI, as R&D FDI is an upgrade decision. I then use data on R&D investment incentives to show that, while governments spend resources on R&D incentives, these can be negative predictors of R&D FDI. The findings imply that government efforts are best directed at incremental encouragement of value-added activities, as efforts to jump to R&D FDI are misguided.
The C-terminal Residues ofSaccharomyces cerevisiaeMec1 Are Required for Its Localization, Stability, and Function
Authors: DaSilva Lance F , Pillon Samantha , Genereaux Julie , Davey Megan J , Gloor Gregory B , Karagiannis Jim , Brandl Christopher J
Published: October 2013

Mec1, a member of the phosphoinositide three-kinase-related kinase (PIKK) family of proteins, is involved in the response to replicative stress and DNA damage and in telomere maintenance. An essential 30 to 35 residue, the FATC domain is found at the C-terminus of all PIKK family members. To investigate the roles of the C-terminal residues of Mec1, we characterized alleles of Saccharomyces cerevisiae mec1 that alter the FATC domain. A change of the terminal tryptophan to alanine resulted in temperature-sensitive growth, sensitivity to hydroxyurea, and diminished kinase activity in vitro. Addition of a terminal glycine or deletion of one, two, or three residues resulted in loss of cell viability and kinase function. Each of these Mec1 derivatives was less stable than wild-type Mec1, eluted abnormally from a size exclusion column, and showed reduced nuclear localization. We identified rpn3-L140P, which encodes a component of the 19S proteasomal regulatory particle of the 26S proteasome, as a suppressor of the temperature-sensitive growth caused by mec1-W2368A. The rpn3-L140P allele acted in a partially dominant fashion. It was not able to suppress the inviability of the C-terminal truncations or additions or the hydroxyurea sensitivity of mec1-W2368A. The rpn3-L140P allele restored Mec1-W2368A to nearly wild-type protein levels at 37°, an effect partially mimicked by the proteasome inhibitor MG-132. Our study supports a role for the C-terminus in Mec1 folding and stability, and suggests a role for the proteasome in regulating Mec1 levels.
Mono and Dual Cofactor Dependence of Human Cystathionine β-Synthase Enzyme Variants In Vivo and In Vitro
Authors: Dimster-Denk Dago , Tripp Katherine W , Marini Nicholas J , Marqusee Susan , Rine Jasper
Published: October 2013

Any two individuals differ from each other by an average of 3 million single-nucleotide polymorphisms. Some polymorphisms have a functional impact on cofactor-using enzymes and therefore represent points of possible therapeutic intervention through elevated-cofactor remediation. Because most known disease-causing mutations affect protein stability, we evaluated how the in vivo impact caused by single amino acid substitutions in a prototypical enzyme of this type compared with physical characteristics of the variant enzymes in vitro. We focused on cystathionine β-synthase (CBS) because of its clinical relevance in homocysteine metabolism and because some variants of the enzyme are clinically responsive to increased levels of its B6 cofactor. Single amino-acid substitutions throughout the CBS protein caused reduced function in vivo, and a subset of these altered sensitivity to limiting B6-cofactor. Some of these B6-sensitive substitutions also had altered sensitivity to limiting heme, another CBS cofactor. Limiting heme resulted in reduced incorporation of heme into these variants, and subsequently increased protease sensitivity of the enzyme in vitro. We hypothesize that these alleles caused a modest, yet significant, destabilization of the native state of the protein, and that the functional impact of the amino acid substitutions caused by these alleles can be influenced by cofactor(s) even when the affected amino acid is distant from the cofactor binding site.
When Life-Sustaining Treatment Is Withdrawn and the Patient Doesn’t Die
Authors: Kutzsche Stefan , Partridge John Colin , Leuthner Steven R. , Lantos John D.
Published: October 2013

One of the most difficult decisions that doctors and parents must make is the decision to withdraw life-sustaining treatment. Doctors find it easier to withdraw treatments in situations where withdrawal will be rapidly fatal rather than in situations in which treatment withdrawal will lead to a prolonged dying process. Mechanical ventilation is usually such a treatment. Withdrawal of ventilation generally leads to the patient’s rapid demise. Doctors may tell parents that death will occur quickly after a ventilator is withdrawn. But what happens when the doctors are wrong and a patient survives without life support? What should doctors do next? We present a case in which that happened and asked 3 experts to comment on the case. Stefan Kutzsche is a senior consultant in neonatology at Oslo University Hospital Ulleval in Norway. John Colin Partridge is a neonatologist and professor of pediatrics at University of California, San Francisco. Steven R. Leuthner is a neonatologist and professor of pediatrics and bioethics at the Medical College of Wisconsin. They each recommend slightly different approaches to this dilemma.
Ponto de colheita e maturação de frutos de camu-camu colhidos em diferentes estádios
Authors: Pinto Patrícia Maria , Jacomino Angelo Pedro , Silva Simone Rodrigues da , Andrade Carolina Amanda Wippich
Published: October 2013

O objetivo deste trabalho foi determinar o ponto de colheita e caracterizar a pós-colheita de frutos de camu-camu (Myrciaria dubia) colhidos em diferentes estádios de maturação. A colheita dos frutos foi realizada em quatro estádios de maturação, definidos pela cor da casca: verde, verde-avermelhada, vermelho-esverdeada e roxa. O armazenamento foi feito em câmaras de refrigeração a 22±1°C e 85±5% UR. Utilizou-se delineamento experimental inteiramente casualizado, em parcelas subdivididas no tempo, com cinco períodos de armazenamento: 0, 3, 6, 9 e 12 dias. Foram avaliados: atividade respiratória; produção de etileno; coloração da casca verificada pelo ângulo de cor e coordenadas de cromaticidade a* e b*; firmeza; perda de massa de matéria fresca; teores de clorofilas, antocianinas, sólidos solúveis e ácido ascórbico; acidez titulável; e incidência de podridão. Os picos de produção de CO2 e etileno ocorreram após a colheita. Os teores de clorofilas e antocianinas variaram com a mudança na coloração da casca de verde para roxa, o que confirmou a maturação dos frutos. Os teores de ácido ascórbico variaram de 759,02 mg por 100 g, no estádio verde, a 1.071,12 mg por 100 g, no roxo. Os frutos colhidos totalmente roxos têm reduzida vida pós-colheita. A maior qualidade pós-colheita do camu-camu é obtida quando os frutos são colhidos com coloração vermelho-esverdeada.
Produção da bananeira 'Prata Anã' em função da aplicação de adubo fosfatado, em quatro ciclos
Authors: Silva José Tadeu Alves da , Rodrigues Maria Geralda Vilela
Published: October 2013

O objetivo deste trabalho foi avaliar a resposta da bananeira 'Prata Anã' (AAB) irrigada à aplicação de fósforo no solo, em quatro ciclos de produção. O experimento foi conduzido na região semiárida do norte de Minas Gerais, em Latossolo Vermelho, argiloso, com teor de P disponível muito baixo (4,6 mg dm-3, Mehlich). Utilizou-se o delineamento de blocos ao acaso, em arranjo fatorial 5x4, com cinco doses de superfosfato triplo (0, 50, 100, 200 e 300 g de P2O5 por planta por ano), quatro ciclos de produção e quatro repetições. No primeiro ciclo, a aplicação de P no solo aumentou a altura, o diâmetro do pseudocaule, a massa e o número de frutos do cacho de banana; no entanto, a bananeira não respondeu à aplicação de P nos segundo, terceiro e quarto ciclos. A bananeira 'Prata Anã', mesmo quando cultivada em solo com baixo teor de P, responde à aplicação desse nutriente somente no primeiro ciclo de produção.
Response of Glyphosate-Tolerant Soybean Yield Components to Dicamba Exposure
Authors: Robinson Andrew P. , Simpson David M. , Johnson William G.
Published: October 2013

Exposure of soybean to dicamba can result in leaf malformation and sometimes yield loss, but it is unclear how yield components are affected by exposure to low quantities of this herbicide. The objectives were to characterize soybean injury and quantify changes in seed yield and yield components of soybean plants exposed to dicamba, and determine if seed yield loss can be estimated from visual injury ratings. Nine dicamba rates (0, 0.06, 0.23, 0.57, 1.1, 2.3, 4.5, 9.1, and 22.7 g ae ha−1) were applied at three growth stages (V2 – two trifoliates, V5-five trifoliates, or R2-full flowering soybean) to Beck's brand ‘342NRR’ soybean planted near Lafayette, IN, in 2009 and 2010 and near Fowler, IN, in 2009. Visually estimated soybean injury of 20% at the V2, V5, or R2 timing was 0.676 to 0.937 g ha−1dicamba at 14 d after treatment (DAT) and 0.359 to 1.37 g ha−1dicamba at 28 DAT. Seed yield was reduced by 5% from 0.042 to 0.528 g ha−1dicamba and a 10% reduction was caused by 0.169 to 1.1 g ha−1dicamba. The number of seeds m−2, pods m−2, reproductive nodes m−2, and nodes m−2were the most sensitive yield components. Path analysis indicated that dicamba reduced seeds m−2, pods m−2, reproductive nodes m−2, and nodes m−2which were the main causes of seed yield loss from dicamba exposure. The correlation of seed yield loss and visual soybean injury was significant (P < 0.0001) for both the V2 treatment timing (R2= 0.92) and the V5 and R2 treatment timings (R2= 0.91). Early-season injury rating of 8% at the V2 treatment and 2% at the V5 or R2 treatments caused 10% or more yield loss.
Postdispersal Loss of Important Arable Weed Seeds in the Midsouthern United States
Authors: Bagavathiannan Muthukumar V. , Norsworthy Jason K.
Published: October 2013

Postdispersal processes play an important role in the regulation of weed population dynamics. Experiments were conducted at two locations in Arkansas to understand postdispersal loss of five arable weed species important to this region—barnyardgrass, johnsongrass, pitted morningglory, Palmer amaranth, and red rice—between seed dispersal in autumn and the production of fresh seeds the subsequent autumn. Total seed loss through predation, decay, germination (fatal or successful), and loss in viability was estimated, and the influences of residue level and seed burial depth (near ground vs. 5 cm deep) were also examined. On average, the active (i.e., viable) seedbank proportion in spring (5 mo after dispersal) ranged from 8 to 11% (barnyardgrass), 10 to 11% (johnsongrass), 20 to 23% (pitted morningglory), 4 to 6% (Palmer amaranth), and 5 to 10% (red rice) across the two locations. At 1 yr after dispersal, 0.7 to 1.5% of barnyardgrass, 7 to 8% of johnsongrass, 5 to 9% of pitted morningglory, about 1.5% of Palmer amaranth, and 0.2 to 0.7% of red rice were part of the active seedbank for the two locations. There was no evidence to suggest that establishing a vegetation cover (such as a rye cover crop) after harvest of the main crop could accelerate seed predation. Burial depth did not influence seed decay, but most (45 [pitted morningglory] to 99% [Palmer amaranth]) of the seeds retrieved from the predator feeding stations were found buried in the soil substrate, and thus, not available for most predator species. This suggests that practices that allow weed seeds to lie on the soil surface (such as no-till planting in autumn) are highly valuable in encouraging seed predation. The high levels of seed loss observed in this study indicate that seedbank management should be a vital component of integrated weed management strategies.
Paleoecology of the K-Pg mass extinction survivor Guembelitria (Cushman): isotopic evidence from pristine foraminifera from Brazos River, Texas (Maastrichtian)
Authors: Ashckenazi-Polivoda Sarit , Rak Carmi , Almogi-Labin Ahuva , Zsolt Berner , Ovadia Ofer , Abramovich Sigal
Published: October 2013

The late Maastrichtian sediments of the Mullinax-1 and Mullinax-3 boreholes from Brazos River, Texas, offer pristine material. These cores are prime candidates for providing an extraordinary window into the ecology of Guembelitria, a key genus in the K/Pg mass extinction event, as well as information on the habitats of other neritic species. Stable oxygen and carbon isotope analyses were performed on six planktic species (Guembelitria cretacea, Globigerinelloides asper, Heterohelix globulosa, Paraspiroplecta navarroensis, Pseudoguembelina costulata, Rugoglobigerina rugosa) and three benthic genera (Gavelinella, Cibicides, and Lenticulina). Our records support the contention that Guembelitria was fully planktic, as indicated by its δ18O values, which overlap the other planktic species, despite its possible origin from a tychopelagic benthic ancestor. However, Guembelitria is distinctly ranked very low in δ13C values, which overlap the benthic records. The anomalously low δ13C values of Guembelitria may represent an isotopic disequilibrium due to fast shell growth, like in its modern analogue Gallitellia vivans. Another explanation may be that these values are attributable to a neustonic life mode in the uppermost part of the oceans, where photosynthesis is inhibited by high UV and the near absence of nutrients. Because these waters are not photosynthetically depleted, calcification using carbon directly from these waters should yield δ13C values consistent with those found in Guembelitria. The ecological strategy that Guembelitria species used to deal with the nutrient-poor surface-water environments was an opportunistic blooming during stressful times of Maastrichtian global warming events and later during the K-Pg catastrophe.
Soil nailing on the A3 Hindhead scheme, UK: a large-scale pre-construction trial
Authors: Davis Andrew J. , Drake Anthony R.
Published: October 2013

The A3 Hindhead scheme in the UK completes the missing dual carriageway link between London and Portsmouth and comprises the construction of 6·4 km of dual carriageway trunk road. As part of the 4·6 km of surface earthworks over 8000 soil nails were installed for both temporary and permanent works. The nailing was undertaken in the Hythe Beds for which limited precedent experience was available and raised site-specific design and construction issues. Due to the scale of the soil nailing, a large pre-construction trial was undertaken to address both design and construction-related issues. The results not only provided confidence in the design, but also invaluable information for construction to aid programming and help ensure high quality construction. This paper describes the trial extent, procedure and results of what could be the largest such trial undertaken in the UK.
Evaluating Sugarcane Families for Yield Potential and Repeatability Using Random Coefficient Models
Authors: Zhou M. M. , Kimbeng C.A. , Andru S. , Tew T. L. , Pontif M. J. , Gravois K. A.
Published: October 2013

Sugarcane (Saccharum species hybrid) is a clonally propagated crop but during variety development, the first stage of selection occurs among seedlings established from true seed. All subsequent stages are established from vegetative stem cuttings and evaluated as clones. During family selection, whole families of seedlings are selected or rejected based on the family mean performance. Individual seedling selection is restricted to selected families. Confounding due to “seed type” can occur when the relative performance of families or individual genotypes differ between the seedling and clonal stages. The objective of this study was to demonstrate the use of random coefficient models (RCMs) for estimating the potential of sugarcane families to produce elite progeny. Data were collected from 17 families evaluated as seedlings in 2002 and clones in 2003. The elite families selected using RCM analysis produced higher mean cane yield in both the seedling and clonal stages and a higher proportion of high yielding clones than those selected using analysis of covariance and analysis of variance. The slope was the most discriminating parameter for family repeatability and was associated with high within‐family variability.