bluuebunny/crossref_metadata_2025 huggingface dataset:
Mechanisms Underlying Aboveground and Belowground Litter Decomposition Converge over Time under Nutrient Deposition
Authors: Jiang Lei , Li Shenggong , Wang Huimin , Dai Xiaoqin , Meng Shengwang , Fu Xiaoli , Zheng Jiajia , Yan Han , Ma Ning , Xue Yafang , Kou Liang
Published: January 2023

Decomposition is vital for nutrient cycling and is sensitive to atmospheric nutrient depositions. However, the influences and underlying mechanisms of nutrient deposition on the long-term decomposition of leaves and absorptive roots remain unclear. Here, we explored the responses of leaves and absorptive roots to nutrient deposition (control, +N, +P, and +NP) in Pinus massoniana and Schima superba forests in subtropical China based on two stages (early-stage (1-year) and late-stage (3-year)) of a decomposition experiment. The chemical factions (acid-unhydrolysable residue (AUR), cellulose, and hemicellulose concentrations) and microbial enzymatic activities (hydrolase and oxidase) were also determined. The +N treatment had persistent negative effects on absorptive root decomposition, except for P. massoniana during the late stage. The +P treatment had a positive effect on leaf decomposition in all stages. The +NP treatment had a positive effect on leaf decomposition during the late stage. The increasing decomposition rates of foliar under +P treatment were more correlated with the increasing acid phosphatase activity than chemical factions, indicating a microbial mechanism. The decreasing decomposition rates of roots under +N treatment were weakly correlated with increasing AUR concentrations and strongly correlated with decreasing oxidase activity during the late stage, indicating both chemical and microbial mechanisms. Overall, our findings highlight that, despite contrasting responses to nutrient deposition, the mechanisms underlying aboveground and belowground decomposition tend to converge as decomposition progresses.
The Construction and Optimization of Habitat Networks for Urban–Natural Symbiosis: A Case Study of the Main Urban Area of Nanjing
Authors: Ding Zhenhui , Cao Jiajie , Wang Yan
Published: January 2023

Maintaining ecological balance relies on biodiversity, and habitat network construction plays an imperative role in preserving biodiversity in regional areas. Nevertheless, there is a problem with the current habitat network construction, which focuses exclusively on ecological benefits without taking other benefits into account as well. In this paper, six species of birds with varying habitat types and varying adaptabilities to city life are selected as target species to build a habitat network based on the InVEST model, Circuit Theory, and Linkage Mapper, focusing on nuclei, patches, corridors, and islands for the harmonious coexistence of human-green space-birds in the most densely populated area of Nanjing, and to refine landscape design techniques for habitat creation. Below is a summary of the main results. Firstly, there is a direct relationship between species distribution and migration capabilities and the urbanization adaptation capabilities of species. Meanwhile, habitat quality has a significant impact on bird species distribution. Furthermore, the habitat network in Nanjing’s main urban area has a distributed and partially degraded core area, a single connectivity structure with poor functionality, and significant fragmentation of habitat patches. Finally, as a result of the above results, two perspectives of ecological landscape planning and design are proposed to optimize the relevant green space landscape in Nanjing’s central urban areas based on biodiversity and satisfying the tripartite symbiosis of humans, green space, and birds in the city. By planning and implementing habitat networks, it is possible to enhance the habitat quality of urban green spaces to a certain extent and provide new ideas for the overall planning of urban–natural systems.
The NtSPL Gene Family in Nicotiana tabacum: Genome-Wide Investigation and Expression Analysis in Response to Cadmium Stress
Authors: He Linshen , Peng Xiang , Cao Hanping , Yang Kunjian , Xiang Lien , Li Rui , Zhang Fangyuan , Liu Wanhong
Published: January 2023

The SQUAMOSA promoter binding protein-like (SPL)SPL family genes play an important role in regulating plant growth and development, synthesis of secondary metabolites, and resistance to stress. Understanding of the role of the SPL family in tobacco is still limited. In this study, 42 NtSPL genes were identified from the genome of the tobacco variety TN90. According to the results of the conserved motif and phylogenetic tree, the NtSPL genes were divided into eight subgroups, and the genes in the same subgroup showed similar gene structures and conserved domains. The cis-acting element analysis of the NtSPL promoters showed that the NtSPL genes were regulated by plant hormones and stresses. Twenty-eight of the 42 NtSPL genes can be targeted by miR156. Transcriptome data and qPCR results indicated that the expression pattern of miR156-targeted NtSPL genes was usually tissue specific. The expression level of miR156 in tobacco was induced by Cd stress, and the expression pattern of NtSPL4a showed a significant negative correlation with that of miR156. These results suggest that miR156-NtSPL4a may mediate the tobacco response to Cd stress. This study lays a foundation for further research on the function of the NtSPL gene and provides new insights into the involvement of NtSPL genes in the plant response to heavy metal stress.
Gene Expression in MC3T3-E1 Cells Treated with Diclofenac and Methylprednisolone
Authors: Lehmann Tomasz P. , Iwańczyk-Skalska Ewa , Harasymczuk Jerzy , Jagodziński Paweł P. , Głowacki Maciej
Published: January 2023

Nonsteroidal anti-inflammatory drugs (NSAIDs) and glucocorticoids (GCs) are often used to treat articular-skeletal disorders. The extended use of NSAIDs and GCs have adverse effects on bone metabolism, reducing bone quality and impairing fracture healing. In the present study, we used mouse pre-osteoblast cells MC3T3-E1 to demonstrate the effects of diclofenac (DF) and methylprednisolone (MP) on cell proliferation and gene expression. Cells were incubated with three doses of DF or MP: 0.5 µM, 5 µM, and 50 µM. MP decreased cell viability even after 24 h, but DF inhibited cell viability after only seven days of treatment. The cells were lysed after one, two, three, and seven days of treatment, and gene expression was analyzed by reverse transcription and quantitative PCR (RT-qPCR) assays. DF did not significantly affect the expression of the osteogenic marker genes. MP modified the expression of Osx, Runx, and Col1a1. We concluded that MP is a more potent inhibitor of mouse pre-osteoblast differentiation and viability than is DF. Our results suggest that prolonged DF treatment could be less harmful to osteoblasts than MP treatment.
Generating Minimal Models of H1N1 NS1 Gene Sequences Using Alignment-Based and Alignment-Free Algorithms
Authors: Fang Meng , Xu Jiawei , Sun Nan , Yau Stephen S.-T.
Published: January 2023

For virus classification and tracing, one idea is to generate minimal models from the gene sequences of each virus group for comparative analysis within and between classes, as well as classification and tracing of new sequences. The starting point of defining a minimal model for a group of gene sequences is to find their longest common sequence (LCS), but this is a non-deterministic polynomial-time hard (NP-hard) problem. Therefore, we applied some heuristic approaches of finding LCS, as well as some of the newer methods of treating gene sequences, including multiple sequence alignment (MSA) and k-mer natural vector (NV) encoding. To evaluate our algorithms, a five-fold cross validation classification scheme on a dataset of H1N1 virus non-structural protein 1 (NS1) gene was analyzed. The results indicate that the MSA-based algorithm has the best performance measured by classification accuracy, while the NV-based algorithm exhibits advantages in the time complexity of generating minimal models.
How Can CpG Methylations, and Pair-to-Pair Correlations between the Main (Gene) and the Opposite Strands, Suggest a Bending DNA Loop: Insights into the 5′-UTR of DAT1
Authors: Di Paola Vincenza , Morrone Martina , Poli Valentina , Fuso Andrea , Pascale Esterina , Adriani Walter
Published: January 2023

A working hypothesis issues from patterns of methylation in the 5′-UTR of the DAT1 gene. We considered relationships between pairs of CpGs, of which one on the main-gene strand and another on the complementary opposite strand (COS). We elaborated on data from ADHD children: we calculated all possible combinations of probabilities (estimated by multiplying two raw values of methylation) in pairs of CpGs from either strand. We analyzed all correlations between any given pair and all other pairs. For pairs correlating with M6-M6COS, some pairs had cytosines positioning to the reciprocal right (e.g., M3-M2COS and M6-M5COS), other pairs had cytosines positioning to the reciprocal left (e.g., M2-M3COS; M5-M6COS). Significant pair-to-pair correlations emerged between main-strand and COS CpG pairs. Through graphic representations, we hypothesized that DNA folded to looping conformations: the C1GG C2GG C3GG and C5G C6G motifs would become close enough to allow cytosines 1-2-3 to interact with cytosines 5-6 (on both strands). Data further suggest a sliding, with left- and right-ward oscillations of DNA strands. While thorough empirical verification is needed, we hypothesize simultaneous methylation of main-strand and COS DNA (“methylation dynamics”) to serve as a promising biomarker.
Estimation of Total Cost Required in Controlling COVID-19 Outbreaks by Financial Incentives
Authors: Kim Sangkwon , Hwang Youngjin , Lee Chaeyoung , Kwak Soobin , Kim Junseok
Published: January 2023

In this article, we present a Monte Carlo simulation (MCS) to estimate the total cost required to control the spread of the COVID-19 pandemic by financial incentives. One of the greatest difficulties in controlling the spread of the COVID-19 pandemic is that most infected people are not identified and can transmit the virus to other people. Therefore, there is an urgent need to rapidly identify and isolate the infected people to avoid the further spread of COVID-19. To achieve this, we can consider providing a financial incentive for the people who voluntarily take the COVID-19 test and test positive. To prevent the abuse of the financial incentive policy, several conditions should be satisfied to receive the incentive. For example, an incentive is offered only if the recipients know who infected them. Based on the data obtained from epidemiological investigations, we calculated an estimated total cost of financial incentives for the policy by generating various possible infection routes using the estimated parameters and MCS. These results would help public health policymakers implement the proposed method to prevent the spread of the COVID-19 pandemic. In addition, the incentive policy can support various preparations such as hospital bed preparation, vaccine development, and so forth.
Neurocognitive Impulsivity in Opiate Users at Different Lengths of Abstinence
Authors: Psederska Elena , Vassileva Jasmin
Published: January 2023

The aim of the current study was to examine the effects of length of abstinence on decision making (impulsive choice) and response inhibition (impulsive action) in former opiate users (OU). Participants included 45 OU in early remission [0–12 months of abstinence], 68 OU in sustained remission [>12 months of abstinence], and 68 control participants. Decision making was assessed with the Iowa Gambling Task (IGT), the Cambridge Gambling Task (CGT), and the Monetary Choice Questionnaire (MCQ). Response inhibition was examined with the Stop Signal Task (SST), and the Go/No-Go Task (GNG). Results revealed group differences in decision making under risk (CGT) and ambiguity (IGT), where control participants displayed better decision making compared to OU in early remission. Both groups of former OU were also characterized by higher discounting of delayed rewards (MCQ). Regression analyses revealed minimal effects of length of abstinence on performance on decision-making tasks and no effects on delay discounting. In addition, both OU groups showed reduced action inhibition (GNG) relative to controls and there were no group differences in action cancellation (SST). Length of abstinence had no effect on response inhibition. Overall, our findings suggest that neurocognitive function may not fully recover even with protracted abstinence, which should be addressed by relapse prevention and cognitive remediation programs for OU.
Healthcare Workers’ Perspectives of mHealth Adoption Factors in the Developing World: Scoping Review
Authors: Addotey-Delove Michael , Scott Richard E. , Mars Maurice
Published: January 2023

Background: mHealth applications provide health practitioners with platforms that enable disease management, facilitate drug adherence, facilitate drug adherence, speed up diagnosis, monitor outbreaks, take and transfer medical images, and provide advice. Many developing economies are investing more in mobile telecommunication infrastructure than in road transport and electric power generation. Despite this, mHealth has not seen widespread adoption by healthcare workers in the developing world. This study reports a scoping review of factors that impact the adoption of mHealth by healthcare workers in the developing world, and based on these findings, a framework is developed for enhancing mHealth adoption by healthcare workers in the developing world. Methods: A structured literature search was performed using PubMed and Scopus, supplemented by hand searching. The searches were restricted to articles in English during the period January 2009 to December 2019 and relevant to the developing world that addressed: mobile phone use by healthcare workers and identified factors impacting the adoption of mHealth implementations. All authors reviewed selected papers, with final inclusion by consensus. Data abstraction was performed by all authors. The results were used to develop the conceptual framework using inductive iterative content analysis. Results and Discussion: Of 919 articles, 181 met the inclusion criteria and, following a review of full papers, 85 reported factors that impact (promote or impede) healthcare worker adoption of mHealth applications. These factors were categorised into 18 themes and, after continued iterative review and discussion were reduced to 7 primary categories (engagement/funding, infrastructure, training/technical support, healthcare workers’ mobile—cost/ownership, system utility, motivation/staffing, patients’ mobile—cost/ownership), with 17 sub-categories. These were used to design the proposed framework. Conclusions: Successful adoption of mHealth by healthcare workers in the developing world will depend on addressing the factors identified in the proposed framework. They must be assessed in each specific setting prior to mHealth implementation. Application of the proposed framework will help shape future policy and practice of mHealth implementation in the developing world and increase adoption by health workers.
How and When May Technostress Impact Workers’ Psycho-Physical Health and Work-Family Interface? A Study during the COVID-19 Pandemic in Italy
Authors: Sommovigo Valentina , Bernuzzi Chiara , Finstad Georgia Libera , Setti Ilaria , Gabanelli Paola , Giorgi Gabriele , Fiabane Elena
Published: January 2023

Although a growing body of research has analyzed the determinants and effects of technostress, it is still unclear how and when technostress would impact workers’ psycho-physical health and work-family interface during the pandemic. To fill this gap, this study tests the mediating mechanisms and the boundary conditions associated with the impact of technostress on workers’ psycho-physical well-being and work-family conflict. A total of 266 Italian workers completed online questionnaires measuring (traditional vs. remote) working modalities, technostress, fear of COVID-19, working excessively, psycho-physical distress, work-family conflict, loss of a loved one due to COVID-19, and resilience. Structural equation models were performed. Results indicated that technostress was positively related to psycho-physical distress and work-family conflict, as mediated by fear of COVID-19 and working excessively, respectively. The loss of a loved one exacerbated the effects of fear of COVID-19 on psycho-physical health, while resilience buffered the effects of working excessively on work-family conflict. Since numerous organizations intend to maintain remote working also after the COVID-19 emergency, it is crucial to study this phenomenon during its peaks of adoption, to prevent its potential negative outcomes. The implications of these findings for theory and practice are discussed.
Examination of Preferences for COVID-19 Vaccines in Hungary Based on Their Properties—Examining the Impact of Pandemic Awareness with a Hybrid Choice Approach
Authors: Blaga Zsanett , Czine Peter , Takacs Barbara , Szilagyi Anna , Szekeres Reka , Wachal Zita , Hegedus Csaba , Buchholcz Gyula , Varga Balazs , Priksz Daniel , Bombicz Mariann , Szabo Adrienn Monika , Kiss Rita , Gesztelyi Rudolf , Romanescu Dana Diana , Szabo Zoltan , Szucs Miklos , Balogh Peter , Szilvassy Zoltan , Juhasz Bela
Published: January 2023

The COVID-19 pandemic has posed a huge challenge to the world in recent years. The development of vaccines that are as effective as possible and accessible to society offers a promising alternative for addressing the problems caused by this situation as soon as possible and to restore the pre-epidemic system. The present study investigated the preferences of residents in Hungary’s second-largest city (Debrecen) for the COVID-19 vaccine. To achieve this aim, a discrete choice experiment was conducted with 1011 participants, and the vaccine characteristics included in the design of the experiment were determined by qualitative methods and a pilot survey: (1) country of origin; (2) efficiency; (3) side effect; and (4) duration of protection. During the data collection at three vaccination sites, respondents were asked to choose between three vaccine alternatives and one “no choice” option in eight decision situations. Discrete choice model estimations were performed using a random parameter logit (RPL) specification with the final model extended to include a latent variable measuring pandemic awareness. The results showed that the vaccine with a Chinese country of origin is the least preferred among the respondents, while the Hungarian and the European vaccines are the most preferred. Furthermore, the increase in the vaccine efficiency level increased the respondents’ sense of utility for the vaccine; the short-term side effect was preferred to the long-term one; and the increase in the duration of protection provided by the vaccine increased the respondents’ sense of utility for the vaccine. Based on the parameter estimated for the latent variable, it can be concluded that as the level of pandemic awareness (which is more positive among people with chronic diseases and less important among health workers) increases, the choice of a vaccine option becomes more preferred among respondents compared to the “no choice“. The results of our investigation could contribute towards increasing compliance in the case of the vaccination-rejecting population, not only for COVID-19, but for any kind of vaccination procedure.
A Review of Manganese-Oxidizing Bacteria (MnOB): Applications, Future Concerns, and Challenges
Authors: Cai Yanan , Yang Kun , Qiu Chaochao , Bi Yunze , Tian Bowen , Bi Xuejun
Published: January 2023

Groundwater serving as a drinking water resource usually contains manganese ions (Mn2+) that exceed drinking standards. Based on the Mn biogeochemical cycle at the hydrosphere scale, bioprocesses consisting of aeration, biofiltration, and disinfection are well known as a cost-effective and environmentally friendly ecotechnology for removing Mn2+. The design of aeration and biofiltration units, which are critical components, is significantly influenced by coexisting iron and ammonia in groundwater; however, there is no unified standard for optimizing bioprocess operation. In addition to the groundwater purification, it was also found that manganese-oxidizing bacteria (MnOB)-derived biogenic Mn oxides (bioMnOx), a by-product, have a low crystallinity and a relatively high specific surface area; the MnOB supplied with Mn2+ can be developed for contaminated water remediation. As a result, according to previous studies, this paper summarized and provided operational suggestions for the removal of Mn2+ from groundwater. This review also anticipated challenges and future concerns, as well as opportunities for bioMnOx applications. These could improve our understanding of the MnOB group and its practical applications.
Measurement of Tourism-Related CO2 Emission and the Factors Influencing Low-Carbon Behavior of Tourists: Evidence from Protected Areas in China
Authors: Wu Jing , Wang Shen , Liu Yuling , Xie Xuesong , Wang Siyi , Lv Lianhong , Luo Hong
Published: January 2023

In the fight against climate change, future policy directions in the transition toward a green travel- and tourism-based economy include improving tourism-derived CO2 emission levels and guiding individual low-carbon behavior. In China, people tend to engage in outdoor adventure travel and cultural tourism in natural areas. However, limited information is available on the empirical evaluation of energy use and the CO2 emissions associated with tourism in protected areas. The present study used a life cycle assessment to explore energy use and CO2 emissions due to tourism and identify the factors driving low-carbon behavior. To these ends, survey data for the protected areas of the Qinling Mountains from 2014 to 2019 were used. The results showed that energy use and CO2 emissions in various tourism sectors steadily increased from 2014 to 2019, primarily because of an increase in transportation activity. This study used data derived from the calculation of CO2 emissions per tourist per trip to identify the various factors jointly contributing to the low-carbon behavior of tourists. These included a low-carbon attitude, low-carbon knowledge, environmental education, and policy reward. The broader implications of this study are that several emission reduction policy options are available to address the challenges inherent in sustainable tourism development and that these policies may be selected according to specific conditions. The low-carbon transformation of recreational facilities at travel destinations, policy rewards, and environmental education can regulate tourist behavior, holding the key to sustainable tourism development in protected areas.
Leveraging Campus Landscapes for Public Health: A Pilot Study to Understand the Psychological Effects of Urban Sheep Grazing on College Campuses
Authors: Kiers A. Haven , Nishimura Kelly M. , Dewa Carolyn S.
Published: January 2023

Since the 1980s, college students in the U.S. have self-reported a decline in their physical and emotional health. With these conditions compounded by the COVID-19 pandemic and its physical distancing restrictions, higher education institutions have an increased responsibility to establish strategic interventions and health-promoting programs for their students. Research collaborations between public health professionals and environmental designers have highlighted the benefits of environmental factors, such as wildlife, street trees, and public parks, on mental health. This pilot project aims to build upon the transdisciplinary dialogue between ecology, design, and public health by examining the social benefits of grazing lawnscape management, which is the practice of using herbivorous livestock to manage turfgrass areas. Through the design of an accessible central campus grazing space for a flock of 25 sheep and use of online questionnaires, a smartphone-based single-item survey, and open-ended feedback given via social media, the UC Davis Sheepmower Project addresses three primary questions: (1) Are there differences in self-reported stress levels and well-being between people who did not watch grazing sheep (no sheepmower group) compared with those who did watch grazing sheep (sheepmower group)? (2) Does holding sheep grazing events create opportunities for education about well-being and engagement with the campus community? (3) Can this type of urban grazing installation ultimately contribute to the overall identity of a college campus? Web-based questionnaire results indicate there is no significant difference in self-reported stress levels between the two groups; however, the moment-in-time smartphone-based single item question suggests that the presence of sheep provides temporary, noticeable relief and enhanced mood for those who observe the animals. Reflections posted on social media suggested that participants found the sheep grazing events fostered feelings of community and placemaking within the campus identity. However, the questionnaire sample indicated the grazing events did not have a significant effect on participants’ sense of place or overall campus identity. This transdisciplinary effort breaks down traditionally siloed approaches to human and environmental health and is an example of a whole-systems approach to developing innovative solutions and encouraging applied collective action.
Analysis of Portuguese Physiotherapists’ Self-Knowledge on Temporomandibular Disorders
Authors: Moleirinho-Alves Paula , Cebola Pedro , Melo Xavier , Simões Sérgio , Godinho Catarina
Published: January 2023

Background: Physiotherapy is one of the most referenced and effective conservative strategies for treating patients with temporomandibular disorders (TMD). This study aimed to characterize and analyze the self-knowledge of TMD of Portuguese physiotherapists. Methods: an online questionnaire was carried out, and the data collected were descriptively analyzed. Results: A total of 338 physiotherapists participated, of which only 142 treated patients with TMD. Seventy-six percent of the physiotherapists reported that they had not received training in the TMD area during the physiotherapy degree course. Only 11% of the physiotherapists reported that treating patients with TMD adequately identified all symptoms of TMD. Conclusions: the present study showed that it is necessary to integrate TMD-related content into the basic training of physiotherapists and promote an increase in evidence-based training.
“The Drug Sellers Were Better Organized than the Government”: A Qualitative Study of Participants’ Views of Drug Markets during COVID-19 and Other Big Events
Authors: Abadie Roberto
Published: January 2023

“Big events”, such as wars, economic crises, pandemics, or natural disasters, affect the risk environment in which people use drugs. While the impact of big events on injection risk behaviors and access to drug-treatment services is well documented, less is known about the effects of big events on drug markets. Based on self-reporting data on drug availability among people who use drugs (PWUD) in the aftermath of Hurricane Maria in Puerto Rico and during the COVID-19 lockdown in a Midwestern US state, this study aims to document the effects of big events on drug markets. Qualitative data on the effects of Hurricane Maria on drug markets are based on participants’ self-reporting (N = 31). Data collection started after the hurricane and ended in 2020. Data on changes to the drug supply during the COVID-19 lockdown were collected based on semi-structured interviews with PWUD (N = 40) in a Midwestern US state. Findings show that while the drug markets might have initially been affected by big events, most effects were temporary. Drug availability, pricing, and quality might have suffered some initial fluctuations but stabilized as the drug markets absorbed the initial shocks caused by the hurricane and the lockdown measures. In preparation for increasingly more frequent and virulent pandemics and natural disasters, health infrastructures should be strengthened to prevent not only overdose episodes and deaths but also drug-related harms.
How Does Living in Temporary Accommodation and the COVID-19 Pandemic Impact under 5s’ Healthcare Access and Health Outcomes? A Qualitative Study of Key Professionals in a Socially and Ethnically Diverse and Deprived Area of London
Authors: Rosenthal Diana Margot , Schoenthaler Antoinette , Heys Michelle , Ucci Marcella , Hayward Andrew , Teakle Ashlee , Lakhanpaul Monica , Lewis Celine
Published: January 2023

Background: Children < 5 years living in temporary accommodation (U5TA) are vulnerable to poor health outcomes. Few qualitative studies have examined service provider perspectives in family homelessness; none have focused on U5TA with a cross-sector approach. This study explored professionals’ perspectives of the barriers and facilitators, including pandemic-related challenges, experienced by U5TA in accessing healthcare and optimising health outcomes, and their experiences in delivering services. Methods: Sixteen semi-structured online interviews were conducted. Professionals working in Newham (London) with U5TA families were recruited from non-profit organisations, the health sector, and Local Authority. A thematic analysis was conducted. Findings: Professionals described barriers including poor parental mental health; unsuitable housing; no social support; mistrust of services; immigration administration; and financial insecurity. Digital poverty, language discordance, and the inability to register and track U5TA made them even less visible to services. Professionals tried to mitigate barriers with improved communication, and through community facilitators. Adverse pandemic effects on U5TA health included delay and regression in developmental milestones and behaviours. In-person services were reduced, exacerbating pre-existing barriers. Interpretation: COVID-19 further reduced the ability of professionals to deliver care to U5TA and significantly impacted the lives of U5TA with potential life-long risks. Innovative and tailored cross-sector strategies are needed, including co-production of public health services and policies focusing on early development, mental health support, employment training, and opportunities for parents/carers.
Prevalence and Geographic Distribution of Self-Reported Chronic Kidney Disease and Potential Risk Factors in Central America
Authors: Figueroa-Solis Erika , Gimeno Ruiz de Porras David , Rojas-Garbanzo Marianela , Whitehead Lawrence , Zhang Kai , Delclos George L.
Published: January 2023

Background: Cases for chronic kidney disease of unknown etiology (CKDu) are increasing in specific disease hotspots located in rural agricultural communities over Central America. The goal of the study was to estimate the prevalence and geographic distribution of self-reported work-related CKD and associated risk factors for CKDu by industry sector in Central America. Methods: We calculated the prevalence and distribution of self-reported CKD, work-related CKD, and suspected CKDu risk factors among the 9032 workers in the Second Central American Survey of Working Conditions and Health (II ECCTS, 2018). We mapped the distribution of suspected CKDu risk factors to work-related CKDu and weather conditions using average annual temperatures. Results: The primary and secondary industry sectors showed the highest proportion of males, suspected CKDu risk factors, and work-related CKD. Age (30–49 years: OR = 2.38, 95% CI 1.03–5.51), ethnicity (mestizo: OR, 7.44, 95% CI: 2.14–25.82), and exposure to high physical work demands (OR = 2.45, 95% CI: 1.18–5.09) were associated with work-related CKD. The majority of work-related CKD were reported in the western parts of Honduras and Nicaragua, in hot temperature regions, and overlapped with those areas with a high density of CKDu risk factors. Finally, some areas clustered CKDu risk factors without any work-related CKD points, mainly in the western part of Guatemala. Conclusion: Our findings supplement prior CKDu findings regarding a high prevalence of work-related CKD among 30- to 49-year-old mestizo males in the primary and secondary sectors, in hot temperature areas, in the central and western region, and overlapping with persons reporting two or more CKDu risk factors. Moreover, several geographic areas with CKDu risk factor clusters had no reported work-related CKD. These areas represent new industries and sectors to be monitored for possible future increases of CKDu cases.
The Effect of Fluid Availability on Consumption and Perceptual Measures during Aerobic Exercise
Authors: Benjamin Courteney L. , Dobbins Luke W. , Sullivan Shealyn G. , Rogers Rebecca R. , Williams Tyler D. , Marshall Mallory R. , Ballmann Christopher G.
Published: January 2023

Fluid availability may alter drinking behavior; however, it is currently unknown if the availability of fluid impacts behavior and gastrointestinal issues (GI) that are often associated with increased fluid intake. The purpose of this study was to determine if ad libitum (AL) versus periodic (PER) fluid intake influences fluid consumption and GI distress during exercise in trained athletes. Male and female Division I NCAA Cross Country athletes (n = 11; age = 20 ± 1 years) participated in this counterbalanced crossover study. Each participant completed a moderate intensity 10 km run on two separate occasions. In one trial, participants had unlimited availability to fluid to consume AL. In the other trial, participants consumed PER fluid at stations placed every 3.2 km. Assurance of euhydration prior to each trial was confirmed via urine specific gravity (USG) and urine color. Subjective perceptions of thirst and gastric fullness were assessed pre- and post-exercise via Likert questioning and a visual analog scale, respectively. Participants started each trial euhydrated (AL = 1.009 USG ± 0.009; PER = 1.009 USG ± 0.009; urine color AL, 3 ± 1; urine color PER, 2 ± 1). Fluid volume consumption was significantly higher during the AL condition compared to PER (p = 0.050). Thirst significantly increased from pre- to post-run regardless of treatment (p < 0.001); however, there was no significant difference between the groups (p = 0.492). Feelings of fullness did not change pre-post trial (p = 0.304) or between trials (p = 0.958). Increased fluid availability allows for increased fluid consumption without the negative experience of GI discomfort.
Infant Low Birth Weight Prediction Using Graph Embedding Features
Authors: Khan Wasif , Zaki Nazar , Ahmad Amir , Bian Jiang , Ali Luqman , Mehedy Masud Mohammad , Ghenimi Nadirah , Ahmed Luai A.
Published: January 2023

Low Birth weight (LBW) infants pose a serious public health concern worldwide in both the short and long term for infants and their mothers. Infant weight prediction prior to birth can help to identify risk factors and reduce the risk of infant morbidity and mortality. Although many Machine Learning (ML) algorithms have been proposed for LBW prediction using maternal features and produced considerable model performance, their performance needs to be improved so that they can be adapted in real-world clinical settings. Existing algorithms used for LBW classification often fail to capture structural information from the tabular dataset of patients with different complications. Therefore, to improve the LBW classification performance, we propose a solution by transforming the tabular data into a knowledge graph with the aim that patients from the same class (normal or LBW) exhibit similar patterns in the graphs. To achieve this, several features related to each node are extracted such as node embedding using node2vec algorithm, node degree, node similarity, nearest neighbors, etc. Our method is evaluated on a real-life dataset obtained from a large cohort study in the United Arab Emirates which contains data from 3453 patients. Multiple experiments were performed using the seven most commonly used ML models on the original dataset, graph features, and a combination of features, respectively. Experimental results show that our proposed method achieved the best performance with an area under the curve of 0.834 which is over 6% improvement compared to using the original risk factors without transforming them into knowledge graphs. Furthermore, we provide the clinical relevance of the proposed model that are important for the model to be adapted in clinical settings.
Analysis of the Barriers and Motives for Practicing Physical Activity and Sport for People with a Disability: Differences According to Gender and Type of Disability
Authors: Ascondo Josu , Martín-López Andrea , Iturricastillo Aitor , Granados Cristina , Garate Iker , Romaratezabala Estibaliz , Martínez-Aldama Inmaculada , Romero Sheila , Yanci Javier
Published: January 2023

The aim of the study was to analyse the perception of the barriers and motives for the practice of physical activity (PA) in people with a disability, according to gender and type of disability. The participants in this investigation were 103 people with a disability (33.25 ± 11.86 years) who were habitual users of PA or sports programmes. They completed the questionnaire Motives and Barriers for Physical Activity and Sport (MBAFD). The results showed that personal barriers were more important than environmental ones and that the most outstanding motives were those related to leisure, enjoyment or social aspects. Regarding gender, women perceived more barriers than men. For the people with physical (PD) and intellectual (ID) disabilities, the most common barriers were of an intrinsic nature, and for those with a visual disability (VD), barriers of an environmental nature. With respect to the motives, people with PD gave higher scores to the items related to aspects of physical improvement and rehabilitation. In contrast, people with ID and VD placed more importance on reasons of leisure, enjoyment and social relations. Knowledge of these findings can be a tool to help increase the provision of PA for people with a disability.
A Systematic Government-Driven Green Development Transformation Strategy with Chinese Characteristics: The Case Study of the Xining Metropolitan Area
Authors: Xu Jing , Yang Yongchun , Jia Zhuo , Chang Genying , Zhang Yongjiao , Zhao Maoyuan , Wang Wenrui
Published: January 2023

In the 21st century, the tension between economic growth, resources and the environment in countries around the world is increasing, and the sustainable development of the economy and society is under great pressure. Green development has become the only way for countries to promote sustainable development. Generally, capitalist countries achieve their green development goals through increasingly strict environmental protection regulations, technological upgrading, industrial upgrading and global transfer based on market mechanisms and legal environments. Evidently, this green development strategy relies on the core position of Western countries in the global technological leadership and the global division of labor. However, limited in terms of their economic strength and by technical barriers, how can developing countries, led by China, in the marginal position in the global market competition, carry out green development transformation? In line with the “high-quality development” strategy, governments at all levels in China are actively exploring green development strategies with their own characteristics. Based on the Second Tibetan Plateau Scientific Expedition and Research and the face-to-face interview method, this paper summarizes a new strategy of systematic government-driven green development combining internal and external factors in the underdeveloped areas of inland China, which has gradually formed in the Xining metropolitan area (XMA) in the past 20 years. This strategy has the following characteristics: Firstly, during the period of rapid growth, the XMA areas have promoted each other through new urbanization and new industrialization and jointly promoted the formation of a green development turn in the new era. Secondly, the government is the core actor and driving force of China’s regional green development and has gradually formulated and implemented a series of policy systems during this development. Restricted by local economic backwardness and low industrial profits, the implementation of green government policies tends to be mandatory. The majority of urban residents and rural people support this transformation because they have benefited from the transformation process. Thirdly, this green development strategy is reflected in many aspects, such as industry, ecology, the environment, space and transportation, and is part of a systematic, green-oriented transformation. Fourthly, the advantages of the socialist system with Chinese characteristics are the guarantee of the green development strategy. It is noteworthy that this kind of green development transformation requires a large amount of “additional” investment and the “rapid” upgrade of the industry. Therefore, it requires more time and the understanding and assistance of all sectors of society.
Assessment of the Possible Correlation between the Presence of Helicobacter Pylori Infection and Hairy Tongue Lesion in a Group of Patients in Syria: A Cross-Sectional and Pilot Study
Authors: Sawan Dania , Mashlah Ammar M. , Hajeer Mohammad Younis , Aljoujou Abeer A.
Published: January 2023

Background: This study aimed to evaluate the correlation between the presence of hairy tongue and H. pylori infection in patients referring to their blood test based on the serum levels of anti-H pylori IgG antibodies. Methods: This cross-sectional study was conducted in the Department of Oral Medicine, University of Damascus Dental School, between February 2021 and January 2022. The sample size of 40 patients (23 males, 17 females), whose ages ranged from 20–79 years with a mean age of 41.5 ± 12 years, was calculated using the G*power 3.1.3, with a statistical power of 80% and a significance level of 0.05. The hairy tongue index was assessed by a visual method based on observing the dorsum tongue appearance. Then, a blood test was performed to detect the presence of H. pylori by Immulite 2000 XPi. Statistical analysis was performed using SPSS software 22.0, Chi-square. Results: The prevalence of hairy tongue was higher among males (75%) as compared to females (25%) and was found to be statistically significant (p = 0.026). The hairy tongue lesions were found to be least in the 20–39 age group and most prevalent in the 40–59 age group, without statistically significant correlation. H. pylori infection was detected positive in 70% and negative in 30% of hairy tongue patients, compared to the control group, where the rates were 15% and 85%, respectively, with a statistically significant correlation between infection with H. pylori and hairy tongue (p = 0.001). Conclusion: Our results strongly suggest that the hairy tongue might be considered an indicator of H. pylori infection.
Diabetes Mellitus Family Assessment Instruments: A Systematic Review of Measurement Properties
Authors: Soares Vânia Lídia , Lemos Sara , Barbieri-Figueiredo Maria do Céu , Morais Maria Carminda Soares , Sequeira Carlos
Published: January 2023

Although many instruments are used to assess the families of people with diabetes, their measurement properties have not been systematically reviewed. We aimed to identify and evaluate the psychometric properties of the instruments used to assess family functioning in adults with diabetes. Methods: A systematic literature review, according to the JBI systematic reviews of measurement properties, was conducted using different databases, including gray literature. PROSPERO registration number: CRD42021239733. Two independent reviewers searched, screened, and assessed the risk of bias among the articles according to the COSMIN methodology. The quality of each included instrument was assessed using the updated criteria for good measurement properties. Results: Eighty-one studies were included, and thirty-one eligible instruments were identified. The psychometric properties frequently assessed were structural validity, internal consistency, and construct validity. Conclusions: Although 31 instruments were included, none of their psychometric properties were scored as “very good”. From the instruments scored as adequate on development and content validity, five stood out for their quality appraisal.. The development of new instruments is not recommended. More studies should be conducted on the existing instruments to assess the less commonly evaluated psychometric properties. Using valid instruments to develop and evaluate interventions is essential to promote health literacy and the effectiveness of diabetes management.
Hydroxychloroquine as an Immunomodulatory and Antithrombotic Treatment in Antiphospholipid Syndrome
Authors: Arachchillage Deepa J. , Laffan Mike , Pericleous Charis
Published: January 2023

Antiphospholipid syndrome (APS) is an acquired highly prothrombotic disorder in which thrombo-inflammatory antiphospholipid antibodies (aPL) cause thrombosis via multiple mechanisms, including endothelial damage and activation. Obstetric complications in APS are caused by placental thrombosis, inflammation and complement activation. Anticoagulation is poorly effective in some patients especially those with triple positive aPL who are at ~30% risk of thrombosis recurrence within 10 years. Increasing therapeutic anticoagulation intensity may be beneficial but leads to excess bleeding with serious complications, such as intracerebral haemorrhage. Nonetheless, anticoagulation is still the mainstay of treatment despite the autoimmune nature of APS. The antimalarial immunomodulatory drug hydroxychloroquine (HCQ) has been used for many years for the treatment of inflammatory rheumatic diseases. HCQ has complex pleiotropic mechanisms of action upon multiple cell types. The proposed biological processes that HCQ regulates support the hypothesis that it may be a successful adjunctive treatment in the prevention of recurrent thrombosis and pregnancy complications.
Bipartite Activation of Sensory Neurons by a TRPA1 Agonist Allyl Isothiocyanate Is Reflected by Complex Ca2+ Influx and CGRP Release Patterns: Enhancement by NGF and Inhibition with VAMP and SNAP-25 Cleaving Botulinum Neurotoxins
Authors: Belinskaia Mariia , Wang Jiafu , Kaza Seshu Kumar , Antoniazzi Caren , Zurawski Tomas , Dolly J. Oliver , Lawrence Gary W.
Published: January 2023

The trafficking of transient receptor potential (TRP) channels to the plasma membrane and the release of calcitonin gene-related peptide (CGRP) from trigeminal ganglion neurons (TGNs) are implicated in some aspects of chronic migraines. These exocytotic processes are inhibited by cleavage of SNAREs with botulinum neurotoxins (BoNTs); moreover, type A toxin (/A) clinically reduces the frequency and severity of migraine attacks but not in all patients for unknown reasons. Herein, neonatal rat TGNs were stimulated with allyl isothiocyanate (AITC), a TRPA1 agonist, and dose relationships were established to link the resultant exocytosis of CGRP with Ca2+ influx. The CGRP release, quantified by ELISA, was best fit by a two-site model (EC50 of 6 and 93 µM) that correlates with elevations in intracellular Ca2+ [Ca2+]i revealed by time-lapse confocal microscopy of fluo-4-acetoxymethyl ester (Fluo-4 AM) loaded cells. These signals were all blocked by two TRPA1 antagonists, HC-030031 and A967079. At low [AITC], [Ca2+]i was limited because of desensitisation to the agonist but rose for concentrations > 0.1 mM due to a deduced non-desensitising second phase of Ca2+ influx. A recombinant BoNT chimera (/DA), which cleaves VAMP1/2/3, inhibited AITC-elicited CGRP release to a greater extent than SNAP-25-cleaving BoNT/A. /DA also proved more efficacious against CGRP efflux evoked by a TRPV1 agonist, capsaicin. Nerve growth factor (NGF), a pain-inducing sensitiser of TGNs, enhanced the CGRP exocytosis induced by low [AITC] only. Both toxins blocked NGF-induced neuropeptide secretion and its enhancement of the response to AITC. In conclusion, NGF sensitisation of sensory neurons involves TRPA1, elevated Ca2+ influx, and CGRP exocytosis, mediated by VAMP1/2/3 and SNAP-25 which can be attenuated by the BoNTs.