bluuebunny/crossref_metadata_2025 huggingface dataset:
Mental Health in Elite Student Athletes: Exploring the Link Between Training Volume and Mental Health Problems in Norwegian College and University Students
Authors: Grasdalsmoen Michael , Clarsen Benjamin , Sivertsen Børge
Published: February 2022

Objectives

To examine mental health problems among elite athletes in a student population, compared to the general student population, and to explore the association between weekly hours of training across mental health indicators.

Methods

Data are from a national study from 2018 of all college and university students in Norway. Participants indicated if they considered themselves to be an elite athlete, and how many hours per week they trained. Mental health problems were assessed using several well-validated questionnaires.

Results

Among 50,054 students, 1.3% identified themselves as elite athletes. Both male and female elite athletes had generally better mental health across most health outcomes, reporting fewer mental health problems, less loneliness, higher satisfaction with life, more positive affect, and fewer alcohol problems. Elite athletes in team sports had slightly better mental health compared to athletes of individual sports. Increased hours of weekly exercise was associated with better mental health. However, there was generally little to be gained from increasing the amount of training from 7–10 hours/week to 14+ hours per week. Female athletes who trained 14 or more hours per week reported poorer mental health across most outcome measures.

Conclusion

This study showed that both male and female elite athletes generally had better mental health across a range of health outcomes, when compared to the general student population. The study also found a positive dose-response relationship between weekly hours of training and mental health, but also a worsening of mental health for females at the extreme end of exercise continuum. The self-report nature of this student sample means that care should be taken when generalizing to other studies of elite athletes.
Innovative Multi-Actor Collaborations as Collective Actors and Institutionalized Spaces. The Case of Food Governance Transformation in Leuven (Belgium)
Authors: Medina-García Clara , Nagarajan Sharmada , Castillo-Vysokolan Lariza , Béatse Erik , Van den Broeck Pieter
Published: February 2022

The sustainable transformation of food systems is a particularly prolific field in which innovative multi-actor collaborations (IMACs) are being tested. Despite the growing literature on food governance and transformation of food systems, and on the principles of alternative food networks and local food strategies, little is known about how these are implemented and how multi-actor networks are coordinated and governed. Taking food as a lens, our objectives are to identify (1) how IMACs are established, (2) how collaboration is enacted within them, and (3) how governance innovations in these fields affect and are affected by broader urban governance innovation. Methodologically, we take a transdisciplinary action research approach, combining different groups of researchers with stakeholders involved in two on-going and interacting IMAC trajectories in Leuven (Belgium), i.e., the non-profit governmental organization Leuven2030 aiming to achieve climate-neutrality and the parallel collective development and implementation of the local food strategy “Food Connects.” After reconstructing the trajectory of these IMACs from a governance perspective and discussing their current limitations, we realize that these IMACs perform both as collective actors and as institutionalized spaces for experimentation and transformative change. Our findings demonstrate that IMACs can be an empowering tool for local actors to challenge supra-local and systemic power imbalance and injustice; that networking and supra-local connections of urban actors can increase the legitimacy and outreach of transition processes, mobilization of resources and peer-learning; and that the consolidation of IMACs builds on previous sedimentations of experimentation and benefits from the impulse of political will in specific windows of opportunity. We conclude that the institutionalization of common objectives set within IMACs requires a parallel institutionalization of governance structures that enabled reaching them, which entails defining specific roles and responsibilities among actors and ensuring stable spaces for conflict, deliberation, and negotiation.
Elephant Endotheliotropic Herpesvirus 4 and Clostridium perfringens Type C Fatal Co-Infection in an Adult Asian Elephant (Elephas maximus)
Authors: Costa Taiana , Rocchigiani Guido , Zendri Flavia , Drake Gabby , Lopez Javier , Chantrey Julian , Ricci Emanuele
Published: February 2022

Elephant endotheliotropic herpesvirus hemorrhagic disease (EEHV-HD) is an acute, often fatal, multisystemic hemorrhagic disease and one of the most significant causes of mortality of Asian elephants in captivity. Most fatal cases of EEHV-HD are associated with EEHV1A and EEHV1B in juveniles. This case report describes the clinical and pathological features of a fatal co-infection of Clostridium perfringens type C and EEHV-HD, caused by EEHV4, in an adult female Asian elephant. Although fatal clostridial enterotoxemia has been occasionally reported in elephants, this report highlights the importance of having both EEHV-HD and clostridial enterotoxemia as potential differential diagnoses in cases of widespread tissue necrosis and internal hemorrhage in elephants, regardless of the animal age group, due to their macroscopic similarities, frequent co-occurrence and cumulative morbid potential.
A Brief Review of Selected Biomechanical Variables for Sport Performance Monitoring and Training Optimization
Authors: Pleša Jernej , Kozinc Žiga , Šarabon Nejc
Published: February 2022

Traditional neuromuscular tests (e.g., jumping and sprinting tasks) are useful to assess athletic performance, but the basic outcomes (e.g., jump height, sprint time) offer only a limited amount of information, warranting a more detailed approach to performance testing. With a more analytical approach and biomechanical testing, neuromuscular function can be assessed in-depth. In this article, we review the utility of selected biomechanical variables (eccentric utilization ratio, force–velocity relationship, reactive strength index, and bilateral deficit) for monitoring sport performance and training optimization. These variables still represent a macroscopic level of analysis, but provide a more detailed insight into an individual’s neuromuscular capabilities, which can be overlooked in conventional testing. Although the aforementioned “alternative” variables are more complex in biomechanical terms, they are relatively simple to examine, with no need for additional technology other than what is already necessary for performing the conventional tests (for example, even smartphones can be used in many cases). In this review, we conclude that, with the exception of the eccentric utilization ratio, all of the selected variables have some potential for evaluating sport performance.
Autophagy in Extracellular Matrix and Wound Healing Modulation in the Cornea
Authors: Kempuraj Duraisamy , Mohan Rajiv R.
Published: February 2022

Autophagy is a robust cellular mechanism for disposing of harmful molecules or recycling them to cells, which also regulates physiopathological processes in cornea. Dysregulated autophagy causes inefficient clearance of unwanted proteins and cellular debris, mitochondrial disorganization, defective inflammation, organ dysfunctions, cell death, and diseases. The cornea accounts for two-thirds of the refraction of light that occurs in the eyes, but is prone to trauma/injury and infection. The extracellular matrix (ECM) is a noncellular dynamic macromolecular network in corneal tissues comprised of collagens, proteoglycans, elastin, fibronectin, laminins, hyaluronan, and glycoproteins. The ECM undergoes remodeling by matrix-degrading enzymes and maintains corneal transparency. Autophagy plays an important role in the ECM and wound healing maintenance. Delayed/dysregulated autophagy impacts the ECM and wound healing, and can lead to corneal dysfunction. Stromal wound healing involves responses from the corneal epithelium, basement membrane, keratocytes, the ECM, and many cytokines and chemokines, including transforming growth factor beta-1 and platelet-derived growth factor. Mild corneal injuries self-repair, but greater injuries lead to corneal haze/scars/fibrosis and vision loss due to disruptions in the ECM, autophagy, and normal wound healing processes. Presently, the precise role of autophagy and ECM remodeling in corneal wound healing is elusive. This review discusses recent trends in autophagy and ECM modulation in the context of corneal wound healing and homeostasis.
Mitochondrial Metabolism, Redox, and Calcium Homeostasis in Pulmonary Arterial Hypertension
Authors: Liang Shuxin , Yegambaram Manivannan , Wang Ting , Wang Jian , Black Stephen M. , Tang Haiyang
Published: February 2022

Pulmonary arterial hypertension (PAH) is a progressive disease characterized by elevated pulmonary arterial pressure due to increased pulmonary vascular resistance, secondary to sustained pulmonary vasoconstriction and excessive obliterative pulmonary vascular remodeling. Work over the last decade has led to the identification of a critical role for metabolic reprogramming in the PAH pathogenesis. It is becoming clear that in addition to its role in ATP generation, the mitochondrion is an important organelle that regulates complex and integrative metabolic- and signal transduction pathways. This review focuses on mitochondrial metabolism alterations that occur in deranged pulmonary vessels and the right ventricle, including abnormalities in glycolysis and glucose oxidation, fatty acid oxidation, glutaminolysis, redox homeostasis, as well as iron and calcium metabolism. Further understanding of these mitochondrial metabolic mechanisms could provide viable therapeutic approaches for PAH patients.
Pathophysiology of Perinatal Asphyxia in Humans and Animal Models
Authors: Mota-Rojas Daniel , Villanueva-García Dina , Solimano Alfonso , Muns Ramon , Ibarra-Ríos Daniel , Mota-Reyes Andrea
Published: February 2022

Perinatal asphyxia is caused by lack of oxygen delivery (hypoxia) to end organs due to an hypoxemic or ischemic insult occurring in temporal proximity to labor (peripartum) or delivery (intrapartum). Hypoxic–ischemic encephalopathy is the clinical manifestation of hypoxic injury to the brain and is usually graded as mild, moderate, or severe. The search for useful biomarkers to precisely predict the severity of lesions in perinatal asphyxia and hypoxic–ischemic encephalopathy (HIE) is a field of increasing interest. As pathophysiology is not fully comprehended, the gold standard for treatment remains an active area of research. Hypothermia has proven to be an effective neuroprotective strategy and has been implemented in clinical routine. Current studies are exploring various add-on therapies, including erythropoietin, xenon, topiramate, melatonin, and stem cells. This review aims to perform an updated integration of the pathophysiological processes after perinatal asphyxia in humans and animal models to allow us to answer some questions and provide an interim update on progress in this field.
The Role of Mid-Regional Proadrenomedullin in the Differential Diagnosis between Culture-Negative and Culture-Positive Sepsis at Emergency Department Admission
Authors: Mearelli Filippo , Barbati Giulia , Spagnol Francesca , Nunnari Alessio , Castello Luigi Mario , Lupia Enrico , Muiesan Maria Lorenza , Di Somma Salvatore , Avanzi Gian Carlo , Biolo Gianni
Published: February 2022

Background: The host response in culture-negative sepsis (CnS) has been marginally explored upon emergency department (ED) admission. It would be of paramount importance to create a clinical prediction rule to support the emergency department physician in identifying septic patients who can be treated with antibiotics immediately without waiting time to draw cultures if they are unlikely to provide useful diagnostic information. Methods: A multivariable logistic regression analysis was applied to identify the independent clinical variables and serum biomarkers of the culture-negative status among 773 undifferentiated septic patients. Those predictors were combined to build a nomogram predictive of CnS. Results: The serum concentrations of six biomarkers, among the eight biomarkers assayed in this study, were significantly lower in the patients with CnS (449) than in those with culture-positive sepsis (324). After correction for co-variates, only mid-regional proadrenomedullin (MR-proADM) was found to be independently correlated with culture-negative status. Absence of diabetes, hemoglobin concentrations, and respiratory source of infection were the other independent clinical variables integrated into the nomogram—its sensitivity and specificity for CnS were 0.80 and 0.79, respectively. Conclusions: Low concentrations of MR-proADM were independently associated with culture-negative sepsis. Our nomogram, based on the MR-proADM levels, did not predict culture-negative status with reasonable certainty in patients with a definitive diagnosis of sepsis at ED admission.
Emerging Treatments for Disorders of Consciousness in Paediatric Age
Authors: Irzan Hassna , Pozzi Marco , Chikhladze Nino , Cebanu Serghei , Tadevosyan Artashes , Calcii Cornelia , Tsiskaridze Alexander , Melbourne Andrew , Strazzer Sandra , Modat Marc , Molteni Erika
Published: February 2022

The number of paediatric patients living with a prolonged Disorder of Consciousness (DoC) is growing in high-income countries, thanks to substantial improvement in intensive care. Life expectancy is extending due to the clinical and nursing management achievements of chronic phase needs, including infections. However, long-known pharmacological therapies such as amantadine and zolpidem, as well as novel instrumental approaches using direct current stimulation and, more recently, stem cell transplantation, are applied in the absence of large paediatric clinical trials and rigorous age-balanced and dose-escalated validations. With evidence building up mainly through case reports and observational studies, there is a need for well-designed paediatric clinical trials and specific research on 0–4-year-old children. At such an early age, assessing residual and recovered abilities is most challenging due to the early developmental stage, incompletely learnt motor and cognitive skills, and unreliable communication; treatment options are also less explored in early age. In middle-income countries, the lack of rehabilitation services and professionals focusing on paediatric age hampers the overall good assistance provision. Young and fast-evolving health insurance systems prevent universal access to chronic care in some countries. In low-income countries, rescue networks are often inadequate, and there is a lack of specialised and intensive care, difficulty in providing specific pharmaceuticals, and lower compliance to intensive care hygiene standards. Despite this, paediatric cases with DoC are reported, albeit in fewer numbers than in countries with better-resourced healthcare systems. For patients with a poor prospect of recovery, withdrawal of care is inhomogeneous across countries and still heavily conditioned by treatment costs as well as ethical and cultural factors, rather than reliant on protocols for assessment and standardised treatments. In summary, there is a strong call for multicentric, international, and global health initiatives on DoC to devote resources to the paediatric age, as there is now scope for funders to invest in themes specific to DoC affecting the early years of the life course.
Increased Threshold and Reduced Firing Rate of Auditory Cortex Neurons after Cochlear Implant Insertion
Authors: Partouche Elie , Adenis Victor , Gnansia Dan , Stahl Pierre , Edeline Jean-Marc
Published: February 2022

The cochlear implant (CI) is the most successful neuroprosthesis allowing thousands of patients with profound hearing loss to recover speech understanding. Recently, cochlear implants have been proposed to subjects with residual hearing and, in these cases, shorter CIs were implanted. To be successful, it is crucial to preserve the patient’s remaining hearing abilities after the implantation. Here, we quantified the effects of CI insertion on the responses of auditory cortex neurons in anesthetized guinea pigs. The responses of auditory cortex neurons were determined before and after the insertion of a 300 µm diameter CI (six stimulating electrodes, length 6 mm). Immediately after CI insertion there was a 5 to 15 dB increase in the threshold for cortical neurons from the middle to the high frequencies, accompanied by a decrease in the evoked firing rate. Analyzing the characteristic frequency (CF) values revealed that in large number of cases, the CFs obtained after insertion were lower than before. These effects were not detected in the control animals. These results indicate that there is a small but immediate cortical hearing loss after CI insertion, even with short length CIs. Therefore, efforts should be made to minimize the damages during CI insertion to preserve the cortical responses to acoustic stimuli.
Barriers to the Implementation of Building Information Modeling among Jordanian AEC Companies
Authors: Hyarat Esraa , Hyarat Tasneem , Al Kuisi Mustafa
Published: February 2022

Building Information Modeling (BIM) offers significant opportunities for construction management as it provides an effective method for design and documentation, supports communication and collaboration, and enhances the most important factors of a project (time, cost, and quality). The aim of this study is to investigate the barriers to BIM implementation at AEC (architecture, management, construction, and quantity survey) companies in Jordan, and to look at the similarities and differences in perceptions on the significance of these barriers between respondents from different AEC companies. To accomplish these objectives, a questionnaire survey was created to gather feedback on the 20 major barriers to BIM implementation identified through a comprehensive literature review. The top significant barriers were identified using the Relative Importance Index (RII) and Mean value; and to assess variances between respondents in the four AEC firms, a one-way analysis of variance (ANOVA) was used. From this, it was concluded that the “cost of training”, “cost of BIM software”, “insufficient BIM technical knowledge and awareness”, “lack of adequate BIM guidelines”, and “huge BIM up frontal investment” are the key barriers affecting the BIM implementation in Jordanian AEC companies. Considering this, this study provides an important step towards better understanding the implementation of BIM in Jordan.
Sound Transmission Loss of a Honeycomb Sandwich Cylindrical Shell with Functionally Graded Porous Layers
Authors: Thongchom Chanachai , Jearsiripongkul Thira , Refahati Nima , Roudgar Saffari Peyman , Roodgar Saffari Pouyan , Sirimontree Sayan , Keawsawasvong Suraparb
Published: February 2022

To examine the acousto-structural behavior of a sandwich cylindrical shell benefiting from hexagonal honeycomb structures in its core and functionally graded porous (FGP) layers on its outer and inner surfaces, a comprehensive study based on an analytical model which also considers the effect of an external flow is conducted. A homogenous orthotropic model is used for the honeycomb core while its corresponding material features are found from the modified Gibson’s equation. The distribution pattern of FGP parts is either even or logarithmic-uneven, and a special rule-of-mixture relation governs their properties. Based on the first-order shear deformation theory (FSDT), Hamilton’s principle is exploited to derive the final coupled vibro-acoustic equations, which are then solved analytically to allow us to calculate the amount of sound transmission loss (STL) through the whole structure. This acoustic property is further investigated in the frequency domain by changing a set of parameters, i.e., Mach number, wave approach angle, structure’s radius, volume fraction, index of functionally graded material (FGM), and different honeycomb properties. Overall, good agreement is observed between the result of the present study and previous findings.
Check and Validation of Building Information Models in Detailed Design Phase: A Check Flow to Pave the Way for BIM Based Renovation and Construction Processes
Authors: Andrich William , Daniotti Bruno , Pavan Alberto , Mirarchi Claudio
Published: February 2022

Model checking of building information models is gaining interest for academic and industrial fields. However, several limitations can be identified in the practical application of model checking processes. One of the main limitations is the unavailability of detailed guidelines describing how these checks should be developed. The research presented in this paper focuses on the development of a check flow that can be applied to any type of building project answering to three main questions, namely what to check, when to perform the check, and who should perform the check. During the research a complete guide for checking activity has been developed identifying the subjects responsible for each specific check during the process. The proposed check list has been tested using a practical case study where the checks have been applied to a real model using commercially available checking tools. The results of the process have determined the need to streamline the proposed flow through a risk management analysis that allowed the definition of two other flows, optimized for achieving prefixed purposes. The research has been developed focusing on a specific phase of the construction process that is the detailed design authorization. Nevertheless, the results can be extended at different phases providing a good support both to clients and public administrations during the validation and authorization phase, and to the designers during the development of the project as an iterative checking process. The results presented in this work can pave the way for the integration of structured and standardized checking processes improving the overall quality of the construction process. This work has been developed and intersected with the activities of the H2020 BIM4EEB project considering the need to pave the way for the creation of quality models to facilitate the development of BIM based renovation processes.
MALAT1 Fusions and Basal Cells Contribute to Primary Resistance against Androgen Receptor Inhibition in TRAMP Mice
Authors: Marhold Maximilian , Udovica Simon , Topakian Thais , Horak Peter , Horvat Reinhard , Tomasich Erwin , Heller Gerwin , Krainer Michael
Published: February 2022

Targeting testosterone signaling through androgen deprivation therapy (ADT) or antiandrogen treatment is the standard of care for advanced prostate cancer (PCa). Although the large majority of patients initially respond to ADT and/or androgen receptor (AR) blockade, most patients suffering from advanced PCa will experience disease progression. We sought to investigate drivers of primary resistance against antiandrogen treatment in the TRAMP mouse model, an SV-40 t-antigen driven model exhibiting aggressive variants of prostate cancer, castration resistance, and neuroendocrine differentiation upon antihormonal treatment. We isolated primary tumor cell suspensions from adult male TRAMP mice and subjected them to organoid culture. Basal and non-basal cell populations were characterized by RNA sequencing, Western blotting, and quantitative real-time PCR. Furthermore, effects of androgen withdrawal and enzalutamide treatment were studied. Basal and luminal TRAMP cells exhibited distinct molecular signatures and gave rise to organoids with distinct phenotypes. TRAMP cells exhibited primary resistance against antiandrogen treatment. This was more pronounced in basal cell-derived TRAMP organoids when compared to luminal cell-derived organoids. Furthermore, we found MALAT1 gene fusions to be drivers of antiandrogen resistance in TRAMP mice through regulation of AR. Summarizing, TRAMP tumor cells exhibited primary resistance towards androgen inhibition enhanced through basal cell function and MALAT1 gene fusions.
Targeting High-Risk Neuroblastoma Patient-Derived Xenografts with Oncolytic Virotherapy
Authors: Quinn Colin H. , Beierle Andee M. , Hutchins Sara Claire , Marayati Raoud , Bownes Laura V. , Stewart Jerry E. , Markert Hooper R. , Erwin Michael H. , Aye Jamie M. , Yoon Karina J. , Friedman Gregory K. , Willey Christopher D. , Markert James M. , Beierle Elizabeth A.
Published: February 2022

Cancer is the leading cause of death by disease in children, and over 15% of pediatric cancer-related mortalities are due to neuroblastoma. Current treatment options for neuroblastoma remain suboptimal as they often have significant toxicities, are associated with long-term side effects, and result in disease relapse in over half of children with high-risk disease. There is a dire need for new therapies, and oncolytic viruses may represent an effective solution. Oncolytic viruses attack tumor cells in two ways: direct infection of tumor cells leading to cytolysis, and production of a debris field that stimulates an anti-tumor immune response. Our group has previously shown that M002, an oncolytic herpes simplex virus (oHSV), genetically engineered to express murine interleukin-12 (mIL-12), was effective at targeting and killing long term passage tumor cell lines. In the current study, we investigated M002 in three neuroblastoma patient-derived xenografts (PDXs). PDXs better recapitulate the human condition, and these studies were designed to gather robust data for translation to a clinical trial. We found that all three PDXs expressed viral entry receptors, and that the virus actively replicated in the cells. M002 caused significant tumor cell death in 2D culture and 3D bioprinted tumor models. Finally, the PDXs displayed variable susceptibility to M002, with a more profound effect on high-risk neuroblastoma PDXs compared to low-risk PDX. These findings validate the importance of incorporating PDXs for preclinical testing of oncolytic viral therapeutics and showcase a novel technique, 3D bioprinting, to test therapies in PDXs. Collectively, our data indicate that oHSVs effectively target high-risk neuroblastoma, and support the advancement of this therapy to the clinical setting.
GPR183 Is Dispensable for B1 Cell Accumulation and Function, but Affects B2 Cell Abundance, in the Omentum and Peritoneal Cavity
Authors: Barington Line , Christensen Liv von Voss , Pedersen Kristian Kåber , Niss Arfelt Kristine , Roumain Martin , Jensen Kristian Høj Reveles , Kjær Viktoria Madeline Skovgaard , Daugvilaite Viktorija , Kearney John F. , Christensen Jan Pravsgaard , Hjortø Gertrud Malene , Muccioli Giulio G. , Holst Peter Johannes , Rosenkilde Mette Marie
Published: February 2022

B1 cells constitute a specialized subset of B cells, best characterized in mice, which is abundant in body cavities, including the peritoneal cavity. Through natural and antigen-induced antibody production, B1 cells participate in the early defense against bacteria. The G protein-coupled receptor 183 (GPR183), also known as Epstein-Barr virus-induced gene 2 (EBI2), is an oxysterol-activated chemotactic receptor that regulates migration of B cells. We investigated the role of GPR183 in B1 cells in the peritoneal cavity and omentum. B1 cells expressed GPR183 at the mRNA level and migrated towards the GPR183 ligand 7α,25-dihydroxycholesterol (7α,25-OHC). GPR183 knock-out (KO) mice had smaller omenta, but with normal numbers of B1 cells, whereas they had fewer B2 cells in the omentum and peritoneal cavity than wildtype (WT) mice. GPR183 was not responsible for B1 cell accumulation in the omentum in response to i.p. lipopolysaccharide (LPS)-injection, in spite of a massive increase in 7α,25-OHC levels. Lack of GPR183 also did not affect B1a- or B1b cell-specific antibody responses after vaccination. In conclusion, we found that GPR183 is non-essential for the accumulation and function of B1 cells in the omentum and peritoneal cavity, but that it influences the abundance of B2 cells in these compartments.
Autophagy and Lysosomal Functionality in CMT2B Fibroblasts Carrying the RAB7K126R Mutation
Authors: Romano Roberta , Del Fiore Victoria Stefania , Saveri Paola , Palamà Ilaria Elena , Pisciotta Chiara , Pareyson Davide , Bucci Cecilia , Guerra Flora
Published: February 2022

Charcot-Marie-Tooth type 2B (CMT2B) disease is a dominant axonal peripheral neuropathy caused by five mutations in the RAB7A gene. Autophagy and late endocytic trafficking were already characterized in CMT2B. Indeed, impairment of autophagy and an increase in lysosomal degradative activity were found in cells expressing the mutant proteins. Recently, we described a novel RAB7 mutation associated with predominantly motor CMT2 and impaired EGFR trafficking. With the aim to analyze the autophagy process and lysosomal activity in CMT2B fibroblasts carrying the p.K126R RAB7 novel mutation and to investigate further the causes of the different phenotype, we have performed Western blot, immunofluorescence and cytometric analyses monitoring autophagic markers and endocytic proteins. Moreover, we investigated lipophagy by analyzing accumulation of lipid droplets and their co-localization with endolysosomal degradative compartments. We found that cells expressing the RAB7K126R mutant protein were characterized by impairment of autophagy and lipophagy processes and by a moderate increase in lysosomal activity compared to the previously studied cells carrying the RAB7V162M mutation. Thus, we concluded that EGFR trafficking alterations and a moderate increase in lysosomal activity with concomitant impairment of autophagy could induce the specific predominantly motor phenotype observed in K126R patients.
Eryptosis: Programmed Death of Nucleus-Free, Iron-Filled Blood Cells
Authors: Dreischer Peter , Duszenko Michael , Stein Jasmin , Wieder Thomas
Published: February 2022

Human erythrocytes are organelle-free cells packaged with iron-containing hemoglobin, specializing in the transport of oxygen. With a total number of approximately 25 trillion cells per individual, the erythrocyte is the most abundant cell type not only in blood but in the whole organism. Despite their low complexity and their inability to transcriptionally upregulate antioxidant defense mechanisms, they display a relatively long life time, of 120 days. This ensures the maintenance of tissue homeostasis where the clearance of old or damaged erythrocytes is kept in balance with erythropoiesis. Whereas the regulatory mechanisms of erythropoiesis have been elucidated over decades of intensive research, the understanding of the mechanisms of erythrocyte clearance still requires some refinement. Here, we present the main pathways leading to eryptosis, the programmed death of erythrocytes, with special emphasis on Ca2+ influx, the generation of ceramide, oxidative stress, kinase activation, and iron metabolism. We also compare stress-induced erythrocyte death with erythrocyte ageing and clearance, and discuss the similarities between eryptosis and ferroptosis, the iron-dependent regulated death of nucleated blood cells. Finally, we focus on the pathologic consequences of deranged eryptosis, and discuss eryptosis in the context of different infectious diseases, e.g., viral or parasitic infections, and hematologic disorders.
Impact of Organelle Transport Deficits on Mitophagy and Autophagy in Niemann–Pick Disease Type C
Authors: Liedtke Maik , Völkner Christin , Hermann Andreas , Frech Moritz J.
Published: February 2022

Defective mitochondria are pathophysiological features of a number of neurodegenerative diseases. Here, we investigated mitochondrial dysfunction in the context of the rare lysosomal storage diseases Niemann–Pick disease type C1 and type C2 (NP-C1 and NP-C2). Mutations in either the NPC1 or NPC2 gene lead to cholesterol accumulation in late endosomes and lysosomes, resulting in impaired cholesterol homeostasis. The extent to which this may lead to mitochondrial dysfunction has been poorly studied so far. Therefore, we investigated the morphology, function, and transport of mitochondria, as well as their degradation via mitophagy, in a disease-associated human neural cell model of NP-C. By performing live cell imaging, we observed markedly reduced mitochondrial transport, although morphology and function were not appreciably altered. However, we observed a defective mitophagy induction shown by a reduced capability to elevate parkin expression and engulf mitochondria in autophagosomes after treatment with carbonyl cyanide 3-chlorophenylhydrazone (CCCP). This was accompanied by defects in autophagy induction, exhibited by a hampered p62 expression and progression, shown by increased LC3BII levels and a defective fusion of autophagosomes and lysosomes. The latter might have been additionally influenced by the observed reduced lysosomal transport. Hence, we hypothesized that a reduced recycling of mitochondria contributes to the pathophysiology of NP-C.
Effects of a Water-Glass Module on Compressive Strength, Size Effect and Stress–Strain Behavior of Geopolymer Recycled Aggregate Concrete
Authors: Wang Qing , Bian Hongguang , Li Mingze , Dai Min , Chen Yanwen , Jiang Hongwei , Zhang Qiang , Dong Fengxin , Huang Jian , Ding Zhaoyang
Published: February 2022

Geopolymer recycled aggregate concrete (GRAC) was prepared by replacing cement with geopolymer and natural aggregate with waste concrete. The effect of the water-glass module on the mechanical properties of GRAC was studied. It was found that water-glass has a double-layer structure. The low module water-glass leads to a thicker diffusion layer and more Na+ and OH− in the solution, which activates more CaO, SiO2, and Al2O3 in the raw material, and improves the strength of GRAC. Moreover, two kinds of gel structures, namely layered C-A-S-H (calcium silicate hydrate) and networked N-A-S-H (zeolite), were found in the products of geopolymer. As the water-glass module changed, the phase of zeolite changed significantly, whereas the calcium silicate hydrate did not change, indicating that the decrease in the water-glass module contributes to the formation of more N-A-S-H gel. The compressive strengths of GRAC with the sizes of 200, 150, and 100 mm3 were in line with Bazant’s size effect theoretical curve. Through the segmented fitting method, the relationship of the size conversion coefficient of GRAC (α), the critical strength (fcr), the critical dimension (Dcr), and the water-glass module (ε) were determined. It was found that ε = 1.5 is the segmented point of the three equations. The elastic modulus and peak stress of GRAC are inversely proportional to the water-glass module, and the peak strain is proportional to the water-glass module, indicating that by reducing the water-glass module, the strength of GRAC can be improved, but the brittleness is increased. The constitutive equation of GRAC with only the water-glass module as a variable was also established. It was found that the polynomial mathematical model and rational fraction mathematical model are optimal for the rising-stage and falling-stage, respectively, and the relationship between the parameters of the rising-stage (a) and the falling-stage (b), and the water-glass module, is given.
Feasibility of Fetal Portal Venous System Ultrasound Assessment at the FT Anomaly Scan
Authors: Nagy Rodica Daniela , Ruican Dan , Zorilă George-Lucian , Istrate-Ofiţeru Anca-Maria , Badiu Anne Marie , Iliescu Dominic Gabriel
Published: February 2022

Objective: To investigate the feasibility of the first trimester (FT) ultrasound scan (US) for the evaluation of the fetal portal venous system (PVS) anatomy, and to evaluate the potential of microcopy for a proper pathology evaluation for the PVS in the FT. Methods: We evaluated the PVS in 200 scan examinations performed in FT pregnancy. Half of the cases were scanned by two operators with extensive experience in obstetric ultrasound—Group I, and the other half was evaluated by two sonographers with less experience—Group II. Second-trimester US and autopsy in terminated pregnancies were used as follow-up. The pathologic evaluation was supported by microscopy. Results: all PVS features were successfully assessed by transabdominal ultrasound (TAUS) in 27% of the Group I cases and 14% in Group II. These rates increased to 88% in Group I and in 72% in Group II, after rescanning and using transvaginal ultrasound (TVUS). The conditions that led to rescanning and TVUS were: BMI greater than 24 in 26% cases, unfavorable fetal position (12.32%), retroverted uterus (12.32%), abdominal scar (10.96%), fibroids (4.11%), and combinations of the above (34.23%). The L-shaped UV confluence was identified transabdominally in 91% in Group I and in 79% in Group II and increased to 98% and 95%, respectively, following reevaluations. Microscopy represented a useful audit in all FT investigated cases. Conclusions: At the end of the FT, the visualization of a normal L-shaped UV confluence, that excludes major PVS abnormalities, is achievable in approx. 80%, indifferently the examiners experience. The sonographers experience, pregnant women BMI, and uterine anomalies as fibroids or retroversion significantly affect the rate of visualization, and necessitates vaginal approach and reexamination. The FT pathology, the audit of the ultrasound findings can only be performed microscopically, with relatively little resources involved and good results.
First-Trimester Diagnosis of Supernumerary Hemivertebra
Authors: Bohiltea Roxana Elena , Ducu Ionita , Mihai Bianca Margareta , Iordache Ana-Maria , Dima Vlad , Vladareanu Emilia Maria , Bacalbasa Nicolae , Bohiltea Alexia-Teodora , Salmen Teodor , Varlas Valentin
Published: February 2022

Hemivertebra is a common cause of congenital scoliosis and results from a lack of formation of one-half of the vertebral body. This condition is very rare and can present as solitary or as a syndrome component: i.e., the split notochord syndrome, which often implies vertebral defects, from a bifid vertebra to hemivertebrae, or fused vertebrae. We describe a case of supernumerary lateral hemivertebra detected prenatally at 12 weeks of gestation and the ultrasonography specifics that lead to early and accurate diagnosis, monitoring during pregnancy, and follow-up at the 4-year period. The case is presented to specify the importance of an early assessment of fetal spine and diagnosis of various conditions, including hemivertebrae, considering the significant association with other anomalies (cardiovascular, urinary, skeletal, gastrointestinal, and central nervous systems), which are most commonly involved. Moreover, the need to counsel future parents on the risks implied by this anomaly is important for the obstetrician. We underline the inclusion of these types of congenital conditions in high-risk pregnancy because of the frequent association with high cesarean delivery rates, growth restriction, delivery before term, and higher morbidity rates.
Aminoglycoside Therapy for Tuberculosis: Evidence for Ototoxicity among Tuberculosis Patients in Ghana
Authors: Owusu Enid , Amartey Benjamin T. , Afutu Emmanuel , Boafo Neal
Published: February 2022

Background: Hearing impairment (HI) is a major problem in Ghana; however, the few attempts at shedding light on its causes appear to overlook the adverse effects of some medications—a gap that this study sought to fill. Aminoglycoside therapy for tuberculosis (TB) treatment is one of these medications. Aim: The aim of this study was to establish the potential of aminoglycoside as a cause of hearing impairment among patients on anti-TB treatment. Method: This was a case–control study, involving patients receiving treatment for TB with aminoglycoside at the chest clinic of the Tema General Hospital and a control group of age- and gender-matched healthy volunteers. A structured questionnaire was administered to obtain the demographic data and case history of the participants. The hearing sensitivity of the participants was assessed using conventional pure tone audiometry and transient evoked otoacoustic emission tests. Results: A hearing loss prevalence of 20% (12/60) was found among patients receiving treatment for TB, with the intensity of impairment ranging from mild to severe. Hearing thresholds of patients receiving anti-TB medications were significantly elevated (p < 0.05) in comparison to the thresholds of the control group, especially at the high frequencies. Conclusion: This study shows that aminoglycoside therapy for tuberculosis may contribute to hearing impairment among tuberculosis patients in Ghana. Audiological management of these patients should therefore be an essential part of their therapeutic treatment plan.
An Experimental and Computational Investigation of Tailor-Developed Combustion and Air-Handling System Concepts in a Heavy-Duty Gasoline Compression Ignition Engine
Authors: Zhang Yu , Kumar Praveen , Pei Yuanjiang , Traver Michael , Popuri Sriram
Published: February 2022

This study investigates using tailor-developed combustion and air-handling system concepts to achieve high-efficiency, clean gasoline compression ignition (GCI) combustion, aimed at addressing a future heavy-duty ultralow NOx standard of 0.027 g/kWh at the vehicle tailpipe and the tightening CO2 limits around the world by combining GCI with a cost-effective engine aftertreatment system. The development activities were conducted based on a 15 L heavy-duty diesel engine. By taking an analysis-led design approach, a first-generation (Gen1) GCI engine concept was developed and tested, encompassing tailor-designed piston bowl geometry, fuel spray pattern, and swirl motion paired with a customized, fixed-geometry, two-stage turbocharging system and a high-pressure EGR loop with two-stage cooling. Across four key steady-state operating points, the Gen1 GCI concept demonstrated 85–95% lower smoke and 2–3% better diesel-equivalent gross indicated fuel consumption compared to the diesel baseline at 1 g/kWh engine-out NOx. By upgrading to a Gen2 air-handling concept that was composed of a prototype, single-stage, variable-geometry turbocharger and a less restrictive EGR loop, 1D system-level analysis predicted that the pumping mean effective pressure was reduced by 43–54% and the diesel-equivalent brake-specific fuel consumption was improved by 2–4%, thereby demonstrating the performance enhancement potential of refining the air-handling system.
Cu/Zn Superoxide Dismutase and Catalase of Yangtze Sturgeon, Acipenser dabryanus: Molecular Cloning, Tissue Distribution and Response to Fasting and Refeeding
Authors: Shi Qingchao , Xiong Xiaoqin , Wen Zhengyong , Qin Chuanjie , Li Rui , Zhang Zhiyong , Gong Quan , Wu Xiaoyun
Published: February 2022

Superoxide dismutase and catalase are two major antioxidant enzymes in the fish antioxidant defense system, which can remove excess reactive oxygen species and protect fish from stress-induced oxidative damage. The present study aimed to clone the sequences of Yangtze sturgeon, Acipenser dabryanus, Cu/Zn superoxide dismutase (AdCu/Zn-SOD) and catalase (AdCAT), and to explore changes of gene expression in the liver and intestine during fasting and refeeding. A total of 120 fish were exposed to four fasting and refeeding protocols (fasting for 0, 3, 7, or 14 d and then refeeding for 14 d). The coding sequences of AdCu/Zn-SOD and AdCAT encoded 155 and 526 amino acid proteins, respectively, both of which were expressed mainly in the liver. During fasting, when compared to the control group, liver AdCu/Zn-SOD expression was significantly higher in the 3- and 14-d groups, whereas its intestinal expression increased significantly only in the 7-d group. Liver AdCAT expression increased significantly in the 3-, 7-, and 14-d groups. During refeeding, liver AdCu/Zn-SOD expression increased significantly in the 3-, 7-, and 14-d groups compared with those in the control group. Similarly, intestinal AdCu/Zn-SOD expression increased significantly in the 3- and 7-d groups. Moreover, intestinal AdCAT expression was significantly higher in the 3-d group than in the control group, but decreased significantly in the 14-d group. Our findings indicated that AdCu/Zn-SOD and AdCAT play important roles in protecting fish against starvation-induced oxidative stress. Yangtze sturgeon exhibited the potential to adapt to a starvation and refeeding regime.
Conserved Signatures in Protein Sequences Reliably Demarcate Different Clades of Rodents/Glires Species and Consolidate Their Evolutionary Relationships
Authors: Gupta Radhey S. , Suggett Carson
Published: February 2022

The grandorder Glires, consisting of the orders Rodentia and Lagomorpha, encompasses a significant portion of the extant mammalian species including Rat, Mouse, Squirrel, Guinea pig and Beaver. Glires species play an important role in the ecosystem and provide valuable animal models for genetic studies and animal testing. Thus, it is important to reliably determine their evolutionary relationships and identify molecular characteristics that are specific for different species groups within the Glires. In this work, we have constructed a phylogenetic tree for >30 genome sequenced Glires species based on concatenated sequences of 25 conserved proteins. In this tree, members of different orders, suborders, and families within Glires formed strongly supported clades, and their interrelationships were also generally reliably resolved. In parallel, we conducted comparative analyses on more than 1500 protein sequences from Glires species to identify highly conserved molecular markers. These markers were comprised of conserved signature indels (CSIs) in proteins, which are specific for different Rodentia/Glires clades. Of the 41 novel CSIs identified in this work, some are specific for the entire Glires, Rodentia, or Lagomorpha clades, whereas many others reliably demarcate different family/suborder level clades of Rodentia (viz. Myomorpha, Castorimorpha, Sciuromorpha, Hystricomorpha, and Muroidea). Additionally, some of the CSIs also provide information regarding the interrelationships among Rodentia subgroups. Our analysis has also identified one CSI that is commonly shared by the Glires and Scandentia species (tree shrew), however, its evolutionary significance is unclear. Several of the identifed rodents-specific CSIs are present in conserved disease-related proteins. Thus, they provide novel molecular markers for genetic and biochemical studies on the functions of these proteins.
Editorial of Special Issue “Geo-Hydrological Risks Management”
Authors: Godone Danilo , Li Changdong , Vick Louise
Published: February 2022

Natural hazards deserve the use of state-of-the-art tools and techniques to cope with them [...]
Emotional Intelligence as a Predictor of Prosocial Behaviors in Spanish and Colombian Older Adults Based on Path Models
Authors: Martí-Vilar Manuel , Trejos-Gil Carlos Andrés , Betancur-Arias Juan Diego
Published: February 2022

Emotional intelligence (EI), empathy, and prosocial behavior (PB) are widely studied in the early stages of life, up to adolescence. However, there have been few studies in older adults. Using a multivariate methodology, exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) were applied with structural equation models (SEM) in 271 older adults in Colombia, along with a Spanish sample made up of 139 adults over 60 years of age, for a total sample of 411 older adults. The results confirmed that EI, as measured with the WLEIS, scale is the best predictor of PB, with excellent adjustment criteria (GFI = 0.99; CFI = 0.98; RMSEA = 0.064; PCLOSE = 0.28; SRMR = 0.023; AIC = 57.30). It is concluded that the path model yielded a reliable predictive explanation of PB, including EI as a key variable that explains prosocial tendencies.