bluuebunny/crossref_metadata_2025 huggingface dataset:
Integrative study reveals the prognostic and immunotherapeutic value of CTCs/CTMs -related prognostic signature in Hepatocellular carcinoma
Authors: Li Xiangyu , Zhao Kai , Lu Yun , Shi Yuanxin , Qiu Peng , Deng Zhengdong , Yao Wei , Wang Jianming
Published: November 2022

Background
Hepatocellular carcinoma (HCC) is the second major cause of tumor death worldwide, threatening people's lives and health. Circulating tumor cells (CTCs) facilitate the progression of many cancers, including HCC.
Methods
Combining transcriptomic data from TCGA, ICGC and ctcRbase databases, we characterized differentially expressed circulating tumor cells/microemboli (CTCs/CTMs) related genes (CRGs). These differentially expressed CRGs were then analyzed with a lasso and multivariate COX, and a five-gene prognostic signature was constructed. Then, the prognostic signature was validated in the ICGC dataset and GES14520 dataset with survival analysis and receiver operating characteristic analysis. The immune infiltration, tumor mutation burden (TMB), tumor immune dysfunction and exclusion (TIDE), and somatic mutation rate were also compared between the high- and low-risk groups to further evaluate the immunotherapeutic value of the model. Molecular subtypes of HCC were characterized by the non-negative matrix factor decomposition method (NMF), and potential compounds were evaluated for different subtypes. Nomograms were constructed to forecast the prognosis of patients. Comparison with other six models to assess the superiority of the CRGS signaturte.
Result
We constructed the prognostic characteristics of five CRGs (including CDCA8, TP53I3, MYCN, HAVCR1, and TXNRD1) and identified two HCC subtypes, C1 and C2. Patients in the low-risk group had a better survival time compared to the high-risk group. And patients in the high-risk group were associated with higher TMB, immune infiltration, somatic mutation rates, and lower TIDE scores. This prognostic profile was validated in the ICGC and GSE14520 datasets and had good predictive performance.
Conclusion
We have studied the perspective of CTC-related genes and developed a new prognostic signature to predict the prognosis of HCC patients. This signature with good performance will help to assess the prognosis of patients and may provide new insights for clinical management strategies.
PARP1 mediated PARylation contributes to myogenic progression
and glucocorticoid transcriptional response.
Authors: Tan Arnold , Younis Awais , Creighton Jade , Evans Alexander , Coveney Clare , Boocock David , Sale Craig , Lavery Gareth , Coutts Amanda , Doig Craig
Published: November 2022

Poly-(ADP-ribose)polymerase 1 (PARP1) enzymatically generates and applies the post-translational modification, as mono (MAR) or poly (PAR) ADP-Ribose (ADPR) chains. PARP1 roles in genome maintenance are well described, but recent work highlight roles in many fundamental processes including cellular identity and energy homeostasis. Herein, we show in both mouse and human skeletal muscle cells that PARP1 mediated PARylation is a regulator of the myogenic program and the muscle transcriptional response to steroid hormones. Chemical PARP1 modulation impacts expression of major myocellular proteins, including troponins, key in dictating muscle contractile force. Whilst PARP1 in absence of DNA damage is often assumed to be basally inactive, we show PARylation to be acutely sensitive to extracellular glucose concentrations and the steroid hormone class, glucocorticoids which exert considerable authority over muscle tissue mass. Specifically, we find during myogenesis, a transient and significant rise in PAR. This early-stage differentiation event, if blocked with PARP1 inhibition, reduced the abundance of important muscle proteins in the fully differentiated myotubes. This suggests that PAR targets during early-stage differentiation are central to the proper development of the muscle contractile unit. We also show that reduced PARP1 in myoblasts impacts a variety of metabolic pathways in line with the recorded actions of glucocorticoids. Currently, as both regulators of myogenesis and muscle mass loss, glucocorticoids represent a clinical conundrum. Our work goes on to identify that PARP1 expression is required for the transcriptional activation by glucocorticoids of a subset of genes critical to human skeletal muscle pathology. These genes may therefore signify a regulatory battery of targets through which selective glucocorticoid modulation could be achieved. Collectively, our data provide clear links between PARP1 mediated PARylation and skeletal muscle homeostatic mechanisms crucial to tissue mass maintenance and endocrine response.
Factors Affecting Students’ Mental Health: A Study of college undergraduates in Nepal
Authors: Ghimire Pratyush , Paudel Hari , Baral Basanta
Published: November 2022

The research aims to create a ‘stressor hierarchy’ by studying the mental stressors (for example: academic bearing, parental pressure etc.) that impact college undergraduates in Nepal. Additionally, with the help of the hierarchy constructed, the research, consequently, seeks to develop a Students Mental Health Index (SMHI) by taking into account the most pernicious stressors that contribute to the decline of students’ mental health across Nepal.
Značaj pojma svijet kao cjelina za bioetiku
Authors: Jevtić Nevena
Published: November 2022

Na osnovi pojma svijet kao cjelina, kako je oblikovan kod Kanta i Hegela, moguće je artikulirati jednu dinamičnu viziju svijeta pomoću koje se adekvatno predočuju međusobne veze različitih faktora. Na taj bi način svaka strana »računice«, s obzirom na koju se promišljaju problemi mainstream bioetike, bila problematizirana u jednom kompleksnom kontekstu međusobnog uvjetovanja. Dijalektička ideja svijeta kao cjeline pomogla bi bioetici steći uvid u način na koji se njeni principi posreduju s principima ostalih poredaka čovjekova djelovanja, što predstavlja pokušaj teorijske podrške svijesti bioetičara o potrebi za globalnim pristupom svojem predmetu. Time što će svoje principe formulirati s obzirom na prijeko potreban globalni pristup, na primjer u vezi s globalnom ekološkom katastrofom, izvjesnije je da će moći postati jednim mehanizmom radikalne promjene naše svakodnevice.
Ludilo kao neadekvatna samosvijest
Authors: Govedarica Milanko
Published: November 2022

U ovom se radu promovira važnost filozofije za razumijevanje ludila, s posebnim naglašavanjem značaja pacijentova egzistencijalnog filozofskog stava za nastajanje i nadilaženje psihotičnog iskustva. Nasuprot faktografskim pristupima karakterističnim za empirijsko spoznavanje relevantnih posebnih znanosti, rasvjetljava se duhovna meta-instanca samonadilaženja, kroz razvijanje teze o ireducibilnosti i nezamjenljivosti čovjekove samosvijesti kako u pogoršanju, tako i poboljšanju mentalnog zdravlja. Nadalje, objašnjava se smisao ispravnog odnosa prema transcendenciji, koji podrazumijeva svijest o ograničenosti spoznaje o apsolutnoj realnosti, ali ne i gubitak nade u njezine spasonosne manifestacije. Pritom se »nedostatak usuglašene autobiografske naracije« i »nesuglasnost s transcendentnim i univerzalnim poretkom vrijednosti« ističu kao glavni kriteriji neadekvatne samosvijesti ludila.
Konformizam kao upotreba znanja – Bernardo Bertolucci, Platon, spilja
Authors: Radonjić Goran
Published: November 2022

Rad ispituje intertekstualne relacije između Bertoluccijeva filma Konformist i Platonove alegorije o spilji. Istaknut je značaj interpretacije za Platonovu alegoriju: ona je središnja i u tematici, i u obliku, i u načinu na koji se izlaže. Interpretacija svijeta neodvojiva je od interpretacije sebe. Koristeći elemente Platonove alegorije o spilji, Bertoluccijev film postaje interpretacija Platona. Istovremeno, u filmu se stvarnost interpretira Platonovim pojmovima i slikama. Preispituju se i pojmovi konformizma, spoznaje i oslobođenja.
¿Existe correlação entre o escore de condição corporal e a taxa de prenhez de vacas nelores submetidas a IATF na Amazônia Oriental?
Authors: Oliveira Vytoria Sanches , Silva Welligton Conceição da , Pina Maia Tomaz , Silva Lílian Kátia Ximenes
Published: November 2022

Objetivou-se avaliar se existe correlação entre o escore de condição corporal (ECC) e a taxa de prenhez de vacas submetidas a inseminação artificial em tempo fixo (IATF) na Ilha do Marajó, Pará, na Amazônia Oriental. Foram analisadas 159 fêmeas bovinas, submetidas à IATF, selecionadas mediante avaliação clínica e ginecológica, sendo consideradas vacas clinicamente saudáveis e cíclicas, da raça Bos taurus indicus, de uma propriedade de sistema extensivo, no município de Cachoeira do Arari, na Ilha do Marajó, Pará. Foi utilizado o método de pesquisa qualitativo, com objetivo de analisar a correlação entra a taxa de prenhez e o ECC, deste lote. Das 159 fêmeas submetidas a IATF, 86 apresentaram diagnóstico de gestação positivo, obtendo-se taxa de prenhez de 54,08% e o número de fêmeas vazias foi de 73, ou seja, 45,92%. Houve correlação positiva (r = 0,167) e significativa entre a ECC e a taxa de prenhez (p<0,05). Desta forma, o estudo permitiu concluir que houve uma interdependência positiva entre a ECC e o diagnóstico gestacional. De modo que constata o efeito da condição corporal sobre a taxa de prenhez em vacas criadas na Amazônia Oriental.
El “barbering”: un problema frecuente en ratones de laboratorio
Authors: Hincapié Juliana Alejandra , Correal Suárez María Lucía , Eduardo Góngora Manuel
Published: November 2022

El barbering es una conducta que consiste en halar o recortar el pelo y en algunos casos incluso ingerirlo. Este comportamiento ha sido reportado tanto en el ser humano como en otras especies, no obstante, su presentación en ratones de laboratorio es frecuente en algunos linajes, genera impacto sobre su bienestar y la fiabilidad de los resultados de investigación, siendo un problema destacado en los bioterios de ratones. Al realizar una revisión de literatura disponible sobre esta condición, se identifican múltiples causas, entre estas, deficiencias en el enriquecimiento ambiental, alteraciones de manejo, estrés, conductas aprendidas, ansiedad y dominancia, así como causas genéticas haciendo que algunas cepas, linajes y modelos genéticamente modificados sean más susceptibles al barbering y ayudando a facilitar la comprensión de rutas metabólicas involucradas en esta condición. La etiología múltiple del barbering permite abordar opciones de manejo orientadas hacia la intervención ambiental y algunas estrategias farmacológicas que podrían ser útiles para reducir su incidencia y los problemas de salud y desempeño reproductivo asociados.
Retention of snowmelt and rain from extensive green roofs during snow-covered periods
Authors: Braskerud Bent C. , Paus Kim H.
Published: November 2022

Green roofs are a popular way to include nature in an urban environment. A reduction in stormwater runoff peaks and volumes are among the benefits one can expect. How is runoff from green roofs in the cold and snow-covered part of the year, when growth media freeze, plants are dormant and covered with melting snow? This paper investigates 11 years of runoff from three green extensive roofs in Oslo, Norway. Precipitation through the snow-covered period (SCP) was approximately one-third of the annual precipitation (970 mm). When runoff from green roofs is compared to runoff from a non-vegetated bitumen roof, retention of 16–31% is seen through the SCP, depending on the drainage system, fabric, soil quality, and depth. The difference in buildup did not influence the detention of the largest runoff intensities. Dampening the runoff happened even though the substrate was saturated. According to the soil moisture sensors, the capacity of the roof with the highest water retention could be increased even more if drainage could be restricted. The runoff from the bitumen roof always exceeded the runoff from green roofs. As a result, harmful inundation may be reduced in a part of the year when infiltration is restricted due to frost.
Anthropogenic nitrate contamination of water resources in Ethiopia: an overview
Authors: Wakejo Wondimu Kebede , Meshesha Beteley Tekola , Habtu Nigus Gabbiye , Mekonnen Yilkal Gebeyehu
Published: November 2022

Nitrate is one of the water contaminants that mainly results from anthropogenic activities. The major causes of nitrate contamination of water resources are anthropogenic activities such as animal or human waste, septic or sewage systems, fertilizer application, concentrated animal farming, industrial waste, and landfill leachates. These man-made activities are the primary sources of nitrate contamination in water resources. Nitrate contamination of water is a global issue that has been increasing over time. According to previous research, exposure to nitrate in water above the World Health Organization (WHO) guideline limit (50 mg of NO3/L) has been found to induce major health effects such as methemoglobinemia in humans, with the severity depending on the amount consumed. This problem has become a major threat to humans and the environment. Thus, this article presented an overview of nitrate contamination of water resources in Ethiopia, emphasizing anthropogenic activities to indicate the current nitrate water contamination status for the necessary remedial actions.
Mucin Level as a Potential Biomarker for Breast Cancer Diagnosis
Authors: Abdihalim Tagwa Sayed , Idris Alkhair Abd Almahmoud
Published: November 2022

Background: Breast cancer is the second leading cause of cancer death and a health problem worldwide. Secreted mucins are upregulated in ductal adenocarcinoma of the breast, however, the use of mucin as breast cancer biomarker has not been established before. This study aimed to determine the use of mucin level as a potential biomarker for breast cancer diagnosis. Materials and methods: This was a retrospective, cross-section study involving 40 women subjects with breast cancer. Mucin level was examined with a combination of Alcian blue/periodic acid Schiff (AB/PAS) technique applied to each specimen. The results obtained were statistically analyzed using SPSS.Results: Results of neutral mucin detection showed that among breast cancer subjects, 16 cases (40%) were neutral mucin score (+), 23 cases (57.5%) were neutral mucin score (++), and 1 case (2.5%) was neutral mucin score (+++). Meanwhile, 10 cases (25%) were acid mucin negative, 17 cases (42.5%) were acid mucin score (+), 11 cases (27.5%) were acid mucin score (++), and 2 cases (5%) were acid mucin score (+++). The most frequent type of mucin was the combination of acid and neutral mucin (30 cases; 75%) and neutral mucin were 10 cases (25%). Conclusion: Detection of mucin level can be used as an alternative technique for the diagnosis of breast cancer complementary to other types of special stains. Keywords: AB, PAS, breast cancer, histological grade, mucin level
Molecular Mechanisms of Notopterygii rhizoma Et Radix for Treating Arrhythmia
Based on Network Pharmacology
Authors: Wei Penglu , Shang Juju , Liu Hongxu , Xing Wenlong , Tan Yupei
Published: November 2022

Objective:

To explore the possible mechanism for treating NRR in arrhythmia using
network pharmacology and molecular docking in this study.

Methods:

Active compounds and targets for NRR were retrieved from the Traditional Chinese
Medicine Systems Pharmacology (TCMSP) Database and Analysis Platform, SymMap, and the
Encyclopedia of Traditional Chinese Medicine (ETCM) databases. Arrhythmia-related genes were
acquired from the Comparative Toxicogenomics Database (CTD) and the GeneCards database.
Overlapping targets of NRR associated with arrhythmia were acquired and displayed via a Venn
diagram. DAVID was applied for GO and KEGG pathway analyses. Cytoscape software and its
plug-in were used for PPI network construction, module division and hub nodes screening. Auto-
Dock Vina and qRT-PCR were carried out for validation.

Results:

In total, 21 active compounds and 57 targets were obtained. Of these, coumarin was the predominant
category which contained 15 components and 31 targets. There were 5 key targets for NRR
in treating arrhythmia. These targets are involved in the apoptotic process, extrinsic apoptotic signaling
pathway in the absence of ligand, and endopeptidase activity involved in the apoptotic process by
cytochrome c. The main pathways were the p53 signaling pathway, Hepatitis B and apoptosis. The
molecular docking and qRT-PCR displayed good effects on hub node regulation in NRR treatment.

Conclusion:

NRR plays an important role in anti-apoptotic mechanisms that modulate the p53 signaling
pathway, which may provide insight for future research and clinical applications focusing
on arrhythmia therapy.
The Determination of Molecular and Toxicological Mechanisms of Cucurbitacin
E in Model Organism Drosophila melanogaster and Various Cancer
Cell Lines: Molecular Modelling, Docking and Dynamic Simulation
Studies
Authors: Tunçbilek Aydın , Azarkan Serap Yalçin , Ercan Fahriye
Published: November 2022

Introduction:

Cucurbitacins are one of the most important components of Ecballium elaterium. Among the cucurbitacins, Cucurbitacin E was the first to be isolated. This study focused on screening the anticancer and insecticidal potential of Cucurbitacin E by the in-vitro, in-vivo, and in-silico methods.

Methods:

In the study, toxicity analysis of Cucurbitacin E were determined on HeLa, Caco 2 cancer cell lines and D. melanogaster. While the expression levels of the BAD, BCL-2, AKT-1 and H-purine genes of cancer cell lines were determined, the CG15530, BUFFY, AKT-1 and Purine genes of D. melanogaster were determined by RT-PCR. Besides, molecular docking and dynamic properties of Cucurbitacin E with human and insectoid enzymes were presented in silico.

Results:

The IC50 value of Cucurbitacin E in the HeLa ovarian and Caco 2 colon cancer cell lines was determined to be 42 ug/ml and 85 ug/ml, respectively. The LC50 and LC99 doses for fruit flies were determined to be 47,693 µg/ml and 133,251 µg/ml, respectively. Gene expression analysis revealed that Cucurbitacin E showed the greatest effect on Purine and AKT-1 genes in D. melanogaster. We analyzed all genes by Western blot, but we did not detect significant changes in genes other than H-purine.
In silico studies revealed that the Purine protein of D. melanogaster had the highest bonding energy with Cucurbitacin E as a ligand. Similarly, Cucurbitacin E showed great affinity towards H-purine (-10.2 kcal/mol). Molecular dynamics simulation studies were also performed to determine the stability of the dynamic process.

Conclusion:

Conclusion: As a result of our in vivo, in vitro and bioinformatic analyzes, it has been seen that Cucurbitacine E is effective against the cancer types and model insect studied.
Recent Reviews on Dynamic Target Detection Based on Vision
Authors: Zhang Hongxin , Song Ruijin , Jiang Hanghang
Published: November 2022

Background:

Vision-based dynamic target detection is an important research topic in
computer vision, which is the basis for intelligent behavior analysis and event detection. Further
research on dynamic target detection methods can help improve target detection and tracking mechanisms
while also driving the development of other related fields. Hence, conducting a review on
vision-based dynamic target detection is very significant.

Objective:

There are many methods for dynamic target detection. This paper introduces their classification,
characteristics, advantages, disadvantages and development trends.

Method:

This paper reviews recent patents and representative articles on dynamic target detection
in simple visual and complex contexts. The crucial methods of these references are introduced from
the aspects of algorithm, innovation, and principle.

Results:

This paper analyzes and compares the existing dynamic target detection methods, summarizes
their characteristics, main applications, and advantages and disadvantages in the current development
stage, and discusses the future development and potential problems of dynamic target tracking
methods.

Conclusion:

Vision-based dynamic target detection can accurately extract moving targets from the
scene. Due to its inherent complexity, each detection method has its advantages and disadvantages
in specific scenes. Currently, the research mainly focuses on the real-time robustness and accuracy
of the algorithm, which needs to be further improved in the aspects of algorithm innovation, multialgorithm
fusion, multi-target recognition, and algorithm adaptability. Therefore, relevant research
patents and documents should be put forward, initiating the future development trend.
Assessment of Parent and Nurse Perceptions of Parental Participation in Hospital Paediatric Care
Authors: Shdaifat Emad , Sudqi Abdallah M. , Abusneineh Firas T. , Al-Ansari Najla M.
Published: November 2022

Background:

Parent and nurse perceptions about the care of hospitalized children have been examined in developed countries, but little is known in developing countries.

Objective:

The study aims to evaluate the perceptions of nurses and parents towards parental participation in care for hospitalized children and associated factors.

Methods:

A cross-sectional design was used to collect data from nurses and parents in paediatric units in the Eastern Region of Saudi Arabia using a convenience sampling technique. Data were collected using the Parent Participation Attitude Scale (PPAS), and the agreement and differences between groups were analysed.

Results:

The present results found that both parents and nurses agreed on 7 out of 20 statements regarding parental involvement in care for their children. Nurses in paediatric surgery had a higher perception of involving parents (P = 0.002), while nurses in Well Baby Nursery (WBN) ward had the lowest perception. The present findings confirm that married caregivers had higher perceptions (P = 0.035) than their single counterparts.

Conclusion:

Parent participation attitude was agreed on for seven statements between parents and nurses. More studies are required to explore the relationship between healthcare providers and parents with regard to parental participation in caring for hospitalized children.
Non-Pharmacological Behaviour Management
Authors: Shetty Raghavendra M. , Pashne Aditi , Gadekar Trisha
Published: November 2022

Behaviour guidance, earlier known as behaviour management, of the child,
is an essential part of paediatric dental practice. Various techniques are used by the
dental health team for effective and efficient dental treatment of the child. At the end of
the treatment, it is necessary to instil a positive dental attitude in the child’s behaviour.
The phrase ‘behaviour management’ has been replaced by ‘behaviour guidance’ in
recent days, but both phrases are used as synonyms in most studies. Various behaviour
management/guidance techniques can be used independently or in combination to
ensure an efficient and effective dental treatment in the child patient. This chapter
describes various behaviours of the child and appropriate non-pharmacological
behaviour guidance techniques which can be employed by the dental health team for a
successful dental treatment, thereby instilling a positive dental attitude in the child.
Diagnosis of Dental Caries
Authors: Widyarman Armelia Sari , Fibryanto Eko , Astoeti Tri Erri
Published: November 2022

A balance between demineralization and remineralization is crucial for
caries progression. Dental caries can progress if demineralization, which is associated
with pathogenic factors, outweighs remineralization, which is linked to protective
factors. In some sites, carious lesions are benign, whereas in others they progress and
invade deep cavities. Dental caries can be classified according to the site where they
occur and their size. Untreated carious lesions can lead to pulpal and periapical
diseases. Treatment plan options are based on the diagnosis (pulpal or periapical).
Growth and Development of Maxilla and Mandible
Authors: Gupta Saraf Bhavna , Sakhare Dhanashree , Kasar Parag
Published: November 2022

Growth is defined as the normal changes in the amount of living substance.
Development refers to all the naturally occurring unidirectional changes in an
individual's life, from its existence as a single cell to its elaboration as a multifunctional
unit terminating in death. It encompasses the normal sequential events between
fertilization and death. Note that development to the biologist refers to the individual in
contradistinction to species. The life of the individual does not start as a gamete or
zygote, but as primordial germ cells that give rise to gametes. In some invertebrates
and plants, life starts from almost any cell which is properly stimulated and maintained.
The term multifunctional unit emphasizes the elaboration of multiple functions rather
than multiple cellularities. Unidirectional changes continue until death. Therefore,
development is a result of a combination of growth, differentiation, and translocation:
These terms are not synonymous.
Cedera olahraga pemain baseball dan softball di Daerah Istimewa Yogyakarta
Authors: Suryobroto Agus Sumhendartin , Budayati Eka Swasta , Anugrah Tama
Published: November 2022

Tujuan penelitian ini untuk mengidentifikasi cedera pada pemain baseball dan softball di Daerah Istimewa Yogyakarta (DIY). Identifikasi meliputi, jenis cedera, bagian tubuh yang cedera dan penyebab terjadi cedera. Jenis penelitian ini deskriptif dengan metode survei. Subjek  yang digunakan pemain baseball dan softball di DIY. Sampel sebanyak 33 orang, 18 pemain baseball dan 15 pemain softball dengan menggunakan teknik pengambilan sampel purposive sampling. Instrumen yang digunakan adalah angket. Teknik analisis data dalam penelitian menggunakan perhitungan statistik deskriptif persentase,   yaitu dengan cara mengadakan persentase dan penyebaran serta memberikan penafsiran yang diperoleh atas dasar persentase tersebut. Hasil penelitian secara keseluruhan terkait cedera pada olahraga baseball dan softball dapat diidentifikasi dari jenis cedera nyeri (sprain/strain) sebesar 29,59 %, cedera memar 24,13 %, lecet 16,36 %, kram dan perdarahan 10,58 %, dislokasi 7,44 %, dan fraktur 1,32 %. Bagian tubuh yang cedera dapat diidentifikasi pada bagian tungkai dan kaki 34,87%, lengan dan tangan 33,60%, badan 24,09%, dan kepala 7,45%. Sedangkan dari segi penyebab cedera dapat diidentifikasi bahwa terkena bola 30,59%, overuse dan terlalu lelah 29,64%, sliding dan jatuh 19,49%, benturan dengan pemain lain 9,67%, lain-lain (sepatu yang sempit, geseran grip bola, dan pegangan bat) 6,18%, kurang pemanasan 3,49%, dan teknik yang salah 0,95%. Sports injury of baseball and softball players in the Special Region of YogyakartaAbstractThe purpose of this study was to identify injuries to baseball and softball players in Special Region of Yogyakarta (DIY). Identification includes, the type of injury, the injured body part and the cause of the injury. This type of research is descriptive with a survey method. Subjects used by baseball and softball players in DIY. The sample is 33 people, 18 baseball players and 15 softball players using purposive sampling technique. The instrument used is a questionnaire. The data analysis technique in this study uses descriptive statistical calculations of percentages, namely by holding percentages and distributions and providing interpretations obtained on the basis of these percentages. The overall results of baseball and softball injuries can be identified by the type of pain injury (strain/strain) 29.59%, bruises 24.13 %, abrasions 16.36%, cramps and bleeding 10.58 %, dislocations 7.44 % , and fracture 1.32%. Injured body parts can be identified as legs and feet 34.87%, arms and hands 33.60%, body 24.09%, and head 7.45%. The causes of injury can be identified that hit by the ball 30.59%, overuse and too tired 29.64%, sliding and falling 19.49%, collision with other players 9.67%, others (narrow shoes, sliding ball grip and bat grip) 6.18%, lack of heating 3.49%, and wrong technique 0.95%.
APRESENTAÇÂO
Authors: Souza Renata Junqueira de , Oliveira Rosemary Lapa de
Published: November 2022

Sabemos que nas sociedades de tradição oral os contadores de histórias fazem uso da palavra também em sua dimensão educativa, uma vez que reconhecem, na oralidade, o principal veículo de transmissão de saberes. Esses contadores têm consciência de que o sujeito já nasce imerso em saberes que lhe atravessam. Nas sociedades em que a escrita tem papel mais central que a oralidade, como a nossa, somos influenciados por uma oralidade secundária, na expressão de Walter Ong (1993), fortemente demarcada por suportes tecnológicos que difundem a voz e a imagem em dimensões diversas. Gislayne Matos (2012), associando a arte de contar histórias com a educação, na perspectiva da dimensão formativa, afirma que o desenvolvimento humano, seja ele razão, sentimento, corporeidade ou espiritualidade, se realiza através da arte de viver. Ela cita a expressão usada por Amadou Hampaté Bá (2010) “do berço ao sarcófago”, para expressar a ideia harmoniosa de “educação formativa”, que começa quando o indivíduo nasce e segue com ele até o final da vida. A narrativa encena, dramatiza a relação expressa por Mikhail Bakhtin quando afirma que: “Eu sou na medida em que interajo com o outro. É o outro que dá a medida do que sou. A identidade se constrói nessa relação dinâmica com a alteridade.” (BAKHTIN, 2003, p. 15). Essas ideias deram mote para o presente número temático1 , que tem inspiração na con[1]tação de histórias, notadamente, em seu viés lúdico-pedagógico, no sentido de ampliar os di[1]zeres proferidos na docência na perspectiva da formação continuada de sujeitos envolvidos na educação de criança, de jovens, de adultos ou de idosos em espaços etnoformativos diversos (MACEDO, 2013). Portanto, o principal objetivo é contribuir com as discussões levantadas em pesquisas relativas a esse objeto de estudo que apresentam interfaces com outras áreas, especificamente, a Arte e a Educação.
Effect of Parents' Parenting Attitudes on Children's Life Satisfaction: Multiple Mediating Effects of Children's Self-Esteem and Depression -Using Data From the 2018 Korean Children and Youth Panel Survey-
Authors: Park Chan Mi , Kim Hyeon Ok
Published: November 2022

Purpose: This descriptive correlation research aimed to confirm the effect of parents’ parenting attitudes on children’s life satisfaction and the multiple mediating effects of self-esteem and depression in the relationship based on secondary data analysis.Methods: This research used data from the Korean Children and Youth Panel Survey 2018, which included a total of 2,596 4th grade elementary school children. The IBM SPSS Statistics ver. 25.0 statistical program analyzed the collected data, and the Process Macro Model 6 analyzed the hypothesis test.Results: Children's self-esteem and depression in the relationship between parents' parenting attitude and children's life satisfaction had a simple mediating effect, and a significant sequential mediating effect of self-esteem and depression.Conclusion: In the relationship between parents' parenting attitude and children's life satisfaction, children's self-esteem and depression were confirmed as simple or sequentially mediated and were important variables for increasing or decreasing children's life satisfaction. Particularly, among the parameters, self-esteem had the greatest mediating effect. A follow-up study is needed to confirm the effect on improving children's life satisfaction by developing a parent education program that considers parental parenting attitudes and children's self-esteem and depression.
Unmasking the Twitter Discourses on Masks During the COVID-19 Pandemic: User Cluster–Based BERT Topic Modeling Approach
Authors: Xu Weiai Wayne , Tshimula Jean Marie , Dubé Ève , Graham Janice E , Greyson Devon , MacDonald Noni E , Meyer Samantha B
Published: November 2022

Background

The COVID-19 pandemic has spotlighted the politicization of public health issues. A public health monitoring tool must be equipped to reveal a public health measure’s political context and guide better interventions. In its current form, infoveillance tends to neglect identity and interest-based users, hence being limited in exposing how public health discourse varies by different political groups. Adopting an algorithmic tool to classify users and their short social media texts might remedy that limitation.

Objective

We aimed to implement a new computational framework to investigate discourses and temporal changes in topics unique to different user clusters. The framework was developed to contextualize how web-based public health discourse varies by identity and interest-based user clusters. We used masks and mask wearing during the early stage of the COVID-19 pandemic in the English-speaking world as a case study to illustrate the application of the framework.

Methods

We first clustered Twitter users based on their identities and interests as expressed through Twitter bio pages. Exploratory text network analysis reveals salient political, social, and professional identities of various user clusters. It then uses BERT Topic modeling to identify topics by the user clusters. It reveals how web-based discourse has shifted over time and varied by 4 user clusters: conservative, progressive, general public, and public health professionals.

Results

This study demonstrated the importance of a priori user classification and longitudinal topical trends in understanding the political context of web-based public health discourse. The framework reveals that the political groups and the general public focused on the science of mask wearing and the partisan politics of mask policies. A populist discourse that pits citizens against elites and institutions was identified in some tweets. Politicians (such as Donald Trump) and geopolitical tensions with China were found to drive the discourse. It also shows limited participation of public health professionals compared with other users.

Conclusions

We conclude by discussing the importance of a priori user classification in analyzing web-based discourse and illustrating the fit of BERT Topic modeling in identifying contextualized topics in short social media texts.
“Constructing Machine Learning models for Orthodontic Treatment Planning: a comparison of different methods.” (Preprint)
Authors: Shojaei Hamid , Augusto Vincent
Published: November 2022

BACKGROUND

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OBJECTIVE

In this study, we investigated the feasibility of leveraging different machine learning methods to help with clinical decision-making on orthodontic treatment planning, including the decision on extraction, extraction pattern and anchorage pattern.

METHODS

Setting and Sample Population: a group of 216 patients (156 extractions and 60 non-extraction) was enrolled.
32 input features were identified and used as the input variable. We proposed seven machine learning methods, including Logistic Regression, SVM, Decision Tree, Random Forest, Gaussian NB, KNN Classifier and Neural Network for extraction and two methods including Random Forest and Neural Network for extraction and anchorage pattern.

RESULTS

For extraction decision, neural network yielded the most promising results with 93 % accuracy, followed by Logistic regression (86 % accuracy and 93% precision), SVM and Random Forest (83 % accuracy and 90% precision). Naïve Bayesian classifier and KNN classifier failed to produce accuracy within the acceptable range, but Naïve Bayes demonstrated the highest recall score with 92 %. For the decision on extraction & anchorage pattern neural network proved to be more reliable, with 89 % accuracy on the extraction pattern and 81% accuracy on the extraction& anchorage pattern. Random Forest classifier with 41% accuracy did not show satisfactory results for this task. The most important features for a decision on extraction in this study were Inter incisal angle, crowding in the mandible, U1-FH, L1-NB and crowding in the maxilla.

CONCLUSIONS

The results demonstrate that neural network can yield considerable accuracy in a medical diagnosis model. However, other algorithms, such as logistic regression and random forest, can also be considered for simpler tasks such as extraction.
Karakterisasi Gen Non Struktural 1 (NSI) Virus Avian Influenza pada Isolat Itik Tahun 2013
Authors: Hidayanto Nur Khusni , Asmara Widya , Wibowo Michael Haryadi
Published: November 2022

Wabah avian influenza (AI) di Indonesia telah terjadi sejak akhir tahun 2003 dan masih terjadi secara endemis sampai sekarang. Pada akhir tahun 2012 terjadi kasus kematian yang cukup tinggi pada itik yang disebabkan penyakit AI subtipe H5N1 yang diklasifikasikan ke dalam clade 2.3.2, sedangkan virus AI sebelumnya diklasifikasikan pada clade 2.1.1, 2.1.2 dan 2.1.3. Salah satu yang berperan dalam virulensi penyakit AI adalah motif asam amino C-terminal protein nonstruktural1 (NS1). Analisis sekuens virus influenza terutama protein nonstruktural 1 (NS1) yang berasal dari unggas mempunyai motif asam amino ESEV pada Cterminal sedang pada virus influenza manusia mempunyai motif RSKV pada C-terminal. Data sekuen NS1 untuk virus AI terbaru belum lengkap sehingga perlu disekuen untuk melengkapi data molekuler NS1 virus AI.Residu C-terminal protein NS1 virus avian influenza subtype H5N1 perlu dikaji karena mempengaruhi patogenisitas dan virulensi virus. Penelitian ini bertujuan untuk mengetahui motif asam amino pada C-terminal protein nonstruktural 1 (NS1) virus avian influenza yang menyerang itik pada tahun 2013. Sampel berasal dari kasus AI pada itik di Tulungagung dan Blitar pada tahun 2013. Isolasi virus menggunakan telur berembrio tertunas ayam. Identifikasi virus AI subtipe H5N1 menggunakan teknik reverse transcription polimerase chain reaction (RT-PCR) dengan primer H5 (Lee et al., 2001) dengan target amplifikasi 545 bp dan primer N1 (Payungporn et al., 2004) dengan target amplifikasi 131 bp. Amplifikasi gen NS1 menggunakan RT-PCR dengan 2 pasang primer (Bannet-Noah et al., 2007) yang didesain mengamplifikasi gen NS dan dilanjutkan proses sekuensing gen NS1. Sekuen yang diperoleh dianalisa dengan menggunakan software MEGA 5.05 yang meliputi multiple alignment, prediksi asam amino dan analisis pohon filogenetik. Hasil sekuen isolat diperoleh panjang nukleotida yang mengkode protein NS1 sepanjang 690 nt. Hasil analisis pohon filogenetik menunjukkan bahwa ke lima isolat uji tidak berada satu grup dengan dengan isolat asal Indonesia tahun 2003- 2008 dan berdekatan dengan klaster virus AI yang berasal dari Asia clade 2.3.2. Pada semua isolat tahun 2013 ditemukan delesi asam amino pada posisi 80-84, substitusi asam amino D92E, asam amino 149 semua isolat mempunyai asam amino alanine (A), asam amino ke 196 ditemukan adanya variasi substitusi berupa lysine (K) dan glutamic acid (E) dan asam amino pada gen NS1 mempunyai motif ESEV pada posisi PDZ ligand.
Deteksi dan Identifikasi Cemaran Virus Avian Influenza pada Pasar Tradisional di Kabupaten Aceh Besar dan Kota Banda Aceh
Authors: Helmi Teuku Zahrial , Yulisma Rika , Panjaitan Budianto , Tabbu Charles Rangga , Haryanto Aris
Published: November 2022

Penelitian ini dirancang untuk mendeteksi keberadaan virus AI pada beberapa titik kritis, yaitu unggas, kandang penampungan sementara, tempat pemotongan dan penjualan karkas, dan mengidentifikasi subtipe virus AI pada beberapa pasar tradisional di Kabupaten Aceh Besar dan Kota Banda Aceh, Propinsi Aceh. Sampel swab diambil secara acak (random) dari empat pasar di Kabupaten Aceh Besar dan Kota Banda Aceh, Propinsi Aceh, yaitu pasar Lambaro, Ketapang, Ulekareng dan Peunayong, dan dipoolkan. Satu pool sampel berisi 1 sampai 5 swab yang dikelompokkan berdasarkan pedagang, jenis unggas, dan titik kritis cemaran AI. Jumlahtotal sampel yang diambil adalah 314 swab, yang terdiri dari swab trakea unggas hidup, kandang penampungan, meja tempat pemotongan, dan karkas, kemudian dikelompokkan dalam 121 pool. Isolasi virus AI kedalam telur ayam bertunas (TAB), pemeriksaan secara serologis (uji HA/HI), dan identifikasi molekuler (metode RT-PCR) di lakukan di bagian Virologi Laboratorium Veteriner, dinas Kesehatan Hewan dan Peternakan Provinsi Aceh, dan Laboratorium Riset Terpadu Fakultas Kedokteran Hewan Universitas Syiah Kuala Banda Aceh. Analisis hasil pemeriksaan serologis dan identifikasi molekuler virus AI dilakukan dengan metode deskriptif. Hasilisolasi virus pada TAB menunjukkan bahwa embrio mengalami kematian pada 1 hari setelah di infeksikan dengan material virus dari sampel swab yang berasal dari pasar Lambaro Kabupaten Aceh Besar, dan titer antibodi terhadap virus AI hanya terdeteksi pada itik dan ayam pedaging yang berkisar antara 24 sampai dengan 27. Dari hasil serologis tersebut dilanjutkan dengan pemeriksaan RT-PCR. Berdasarkan hasil uji RT-PCR menggunakan primer spesifik terhadap gen M, H5, dan N1 diperoleh virus AI yang bersirkulasi pada unggas di pasar Lambaro tergolong subtipe H5N1. Hasil penelitian ini membuktikan bahwa diantara beberapa titik kritiscemaran virus AI di pasar tradisional, unggas hidup (itik dan ayam pedaging) yang berada dalam kandang penampungan sementara merupakan potensial penyebaran virus AI ke lingkungannya.
Fucoidan : Manfaat dalam Industri Peternakan
Authors: Yanuartono Yanuartono , Nururrozi Alfarisa , Purnamaningsih Hary , Indarjulianto Soedarmanto , Raharjo Slamet
Published: November 2022

Research of interest marine algae and seagrasses have been renewed, because they are considered to be promising resources of functional ingredients in the development of novel products. Fucoidans are a class of fucose-rich sulfated carbohydrates found in brown marine algae and echinoderms, and more recently identified in seagrasses. Chemical compositions of most fucoidans are complex, mainly being composed of fucose and sulfate. They also contain other monosaccharides like mannose, galactose, glucose, xylose and uronic acids, even acetyl groups and protein and it’s have potential therapeutic properties, including anti-viral activity. Currently, fucoidans are available for use in therapeutic and dietary supplements for inclusion in pet, livestock and aquaculture feed supplements. This review summarizes experimental evidence indicating that Fucoidan can improve the quality of feed ingredients for livestock, pets and aquaculture to improve health.
Cytotoxic Tobacco Smoke in The Cornea of Wistar Rats Fed Turmeric (Curcuma Domestica Val.) Extract
Authors: Kusumawardani Agapietta , Sarwendah Kurniati , Rahmad Laelatul , Milah Nida Ul , Herliyani Novi , Sutrisno Bambang , Wuryastuty Hastari , Wasito R
Published: November 2022

Abstract An increasing number of smokers who tend to rise rapidly from year to year has become a major focus of public health issues. Exposure to environmental tobacco smoke, containing toxic chemicals can trigger acute or chronic inflammation and oxidative stress in the eyes. To overcome this condition, phytotherapeutic and/or phytopreventive approaches which are efficatious, safe and cheap is needed. One of spices from Indonesia which is greatly possible to be promoted as a herbal medicine, is a phytochemical curcumin in turmeric. This present study was conducted to identify and determine the effects of turmeric extract (Curcuma domestica Val.) as phytotherapeutic and/or phytopreventive in the eyes lesion of Wistar rats induced by cigarette smokes. Twenty male Wistar rats at 3 months old were divided randomly into four groups of five each. Those were the control group (KI), the group given 9 cigarettes smoke/day (K II), the group given turmeric extract 1 ml 0, 25% /day (K III) and the group given 9 cigarettes smoke/day and 0, 25% turmeric extract 1 ml /day (K IV) for 60 days. Water and feed was given ad libitum. At the end of the experiment, all rats were euthanized with ether, necropsied andeyes balls were collected, fixed formalin 10% and stained with routine hematoxylin-eosin. The results of the present study indicated that 9 cigarettes smoke/day increased mitotic activity and caused hyperplasia in the corneal epithelial cells (K II), and addition of turmeric extract 0, 25% 1 ml per oral for 60 days prevented both mitotic activity and hyperplasia in the corneal epithelial cells (K IV). Extract of turmeric (Curcuma domestica Val.) has been proved to have herbal therapeutic and/or preventive effects againts the pathotoxicological cigarette smokes. Further study needs to be done to more evaluate pathogenesis of the eyes lesions and phytotherapeutic and/or phytopreventive effects of turmeric extract as well.
Hypertropic Osteopathy (Marie Diseases) in The German Shepherd Dog
Authors: Adji Dhirgo
Published: November 2022

Abstract A Male German Shepherd dog, five years old, twenty six kg of body weight was submitted to Professor Soeparwi Animal Hospital, Faculty of Veterinary Medicine, Gadjah Mada University by Mr. Siswo Han, the owner who lives in Cangkringan, Yogyakarta on May 13, 2013. The dog was suffered from the pain and lameness on the front-left extremity for about 2 weeks. The dog having registered number of 8738/V/A/RSH/13 did not show any specific clinical signs, except the lameness. Skin and hairs, mucous membranes, lymphoid glands, and respiratory, digestive, circulatory and uropoetica organs as well were within the normal limits. The result of standard blood test for both red blood cell and white blood cell were still within normal values. Evaluation of fecal sample of the dog was also normal meaning that no parasites were found in the fecal sample. Radiographic analysis of the upper left extremity and the lungs area showed that there was an irregular bone growth on the left caput humeri, and the lungs were normal. Based on the lesion, the dog was diagnosed as having a hyperthropic osteopathy (Marie Disease).
Kandidat Probe Parsial Genom Eimeria tenella Untuk Optimalisasi Diagnosis Koksidiosis = Probe Candidate of Eimeria tenella Partial Genome to Optimalize Coccidiosis Diagnose
Authors: . Sumartono
Published: November 2022

Penelitian ini bertujuan untuk mencari kandidat probe yang dapat digunakan untuk mengoptimalisasi diagnosis koksidiosis berdasarkan fragmen genom Eimeria tenella. Kandidat tersebut ditentukan berdasarkan analisis DNA dari 5 isolat Eimeria tenella ( Jepang, Yogya, Magelang, Solo dan Boyolali ). Isolasi DNA dilakukan dengan metode fenol-khloroformisoamilalkohoI, sedang amplifikasi fragmen DNA dilakukan dengan metode PCR menggunakan forward primer GCTGTGGCCAGAGCAAC dan reverse primer CAACTCCATCGGGCCCA. Sekuensing produk PCR yang ukurannya sarna besar dilakukan di laboratorium Eijkman dan analisis sekuen dilakukan dengan Gene-analyser Genetyx-Max versi 9. Kesimpulan penelitian adalah bahwa sekuen GGCACAGTATCCTCCTTCAGGGCAGGGCT CGCACTGGTCAAACGCGGTA CCATT dan GCCTAAA.TTCATGACCACACACTA merupakan kandidat probe parsial genom E. tenella untuk pengembangan diagnosis koksidiosis.