bluuebunny/crossref_metadata_2025 huggingface dataset:
Influence of Soil Wetting and Drying Cycles on Soil Detachment
Authors: Wang Jian , Watts Dexter B. , Meng Qinqian , Ma Fan , Zhang Qingfeng , Zhang Penghui , Way Thomas R.
Published: June 2022

Agricultural soils undergo periods of saturation followed by desiccation throughout the course of a growing season. It is believed that these periods of wetting and drying influence soil structure and may affect the rate of soil detachment. Thus, an experiment was conducted to investigate the influence of a disturbed soil (soil sieved to simulate tillage) subjected to various wetting and drying cycles, on soil bulk density and the resistance to soil detachment with runoff. Seven treatments consisting of wetting and drying cycles ranging from 0 to 6 cycles were evaluated under laboratory conditions using an experimental flume apparatus. A Richards growth model proposed for predicting the influence of wetting and drying on soil detachment was also evaluated. Results showed that the soil bulk density increased as the number of wetting and drying cycles increased. The soil detachment rate decreased as the number of wetting and drying cycles increased. Moreover, initial soil detachment (occurring as soon as runoff began) rates were high for 1 to 3 wetting and drying cycles, while the rate of initial detachment decreased after the third cycle. For example, soils with two and three wetting and drying cycles took 6.5 and 7 min to reach the maximum 1 cm souring depth, respectively, while the soils subjected to four or more wetting and drying cycles did not reach the maximum 1 cm depth during the 15 min runoff experiment. In addition, the proposed S-Shaped Richards growth model was a good predictor for estimating the soil detachment of soils experiencing various wetting and drying cycles. Findings from this study suggest that more attention should be given to the influence that soil wetting and drying have on the prediction of soil detachment. Information from this study is expected to be useful for improving soil management strategies for reducing soil erosion.
Low Plant Density Improves Fruit Quality without Affecting Yield of Cucumber in Different Cultivation Periods in Greenhouse
Authors: Ding Xiaotao , Nie Wenfeng , Qian Tingting , He Lizhong , Zhang Hongmei , Jin Haijun , Cui Jiawei , Wang Hong , Zhou Qiang , Yu Jizhu
Published: June 2022

With the development of the economy, the demand for cucumber quality is quickly increasing. The aim of this study was to elucidate the role that plant density plays in leaf photosynthesis, shoot dry matter distribution, yield and quality of cucumber in different cultivation periods under greenhouse conditions. Experimental treatments based on three plant density treatments (2.25, 3.0 and 3.75 plants m−2) were conducted in turn during three growth and harvest periods in a year. The results showed that the changes in photosynthesis and weekly yield per unit area were different and dependent on the harvest time, which was mainly induced by temperature and radiation. Interestingly, we found that reducing plant density did not significantly affect the photosynthesis of leaves and did not decrease weekly yield per unit area and total yield. Low-density treatment had the highest weekly yield per plant and total yield per plant in the three harvest periods, the highest ratio of dry matter being allocated to fruits and the highest contents of soluble sugar, total phenols, flavonoid, soluble protein, vitamin C (Vc), chlorophyll and carotenoids in fruits. Moreover, a relatively low nitrite content was found in fruits following low-density treatment. The study indicated that low-density treatment was associated with a high quality of fruits without reducing the cucumber’s total annual yield under natural light in the greenhouse. Hence, our study suggests that properly reducing cucumber plant density to 2.25 plants m−2 could be a practicable approach for greenhouses in Shanghai, China.
Salicylic Acid Improves the Salt Tolerance Capacity of Saponaria officinalis by Modulating Its Photosynthetic Rate, Osmoprotectants, Antioxidant Levels, and Ion Homeostasis
Authors: Xu Lingxin , Chen Hong , Zhang Tingting , Deng Yanan , Yan Junxin , Wang Lei
Published: June 2022

Salicylic acid (SA) plays an important role in regulating salt stress tolerance in plants. However, there are no studies on the effect of exogenous SA on Saponaria officinalis under salt stress. To study the effectiveness of SA on mitigating salt stress, S. officinalis were used in a pot experiment of salt stress simulated with an NaCl solution (100, 200, and 300 mmol L−1), while an SA solution (0, 0.2, 0.4, 0.6, 0.8, 1.0 mmol L−1) was sprayed on leaves. Under salt stress, spraying SA caused an increase in the salt damage index, electrolyte leakage, and a reduction in malondialdehyde and Na+ content, but an increase in the rate of photosynthesis, chlorophyll, soluble sugar, soluble protein, free proline, K+, Mg2+, Ca2+ content, the K+/Na+ ratio, superoxide dismutase, peroxidase, catalase, ascorbate peroxidase activity, and the comprehensive score. The results show that SA improves the salt tolerance capacity of S. officinalis by modulating its photosynthetic rate, osmoprotectants, antioxidant levels, and ion homeostasis. However, the effectiveness of SA was not linearly related to its concentration. In summary, our findings reveal the protective roles of SA against salinity in S. officinalis and suggest that the use of 0.6 mmol L−1 of SA in salt stress conditions could be an effective approach to reduce the damage caused by saline soil in S. officinalis.
Analysis of Relationship between Soybean Relative Maturity Group, Crop Heat Units and ≥10 °C Active Accumulated Temperature
Authors: Wen Huiwen , Wu Tingting , Jia Hongchang , Song Wenwen , Xu Cailong , Han Tianfu , Sun Shi , Wu Cunxiang
Published: June 2022

Crop heat units (CHU) and ≥10 °C active accumulated temperature (≥10 °C AAT) are important indexes to quantify the effects of temperature on soybean development. The relative maturity group (RMG) is widely used in the classification of different soybean varieties. However, CHU and ≥10 °C AAT (AAT) were applied in Canada and northeastern China, respectively, and the relationships among CHU, AAT and RMG are poorly documented. The objective of this study is to analyze the conversion function among CHU, AAT and RMG based on two datasets. The first dataset was obtained to analyze the relationship between RMG and AAT in 395 varieties in Northeast China. The second dataset was obtained to calculate the relationship between CHU and AAT at 95 weather stations based on 30-year climatic data (1990–2019). The results showed that both relationships were significantly and positively correlated, and the R-square of these relationships were 0.90 and 0.98, respectively. The distribution of CHU or AAT in the Northeast is proposed. These results can be extensively used for predicting the CHU or AAT of soybean cultivars given the known RMG, thus determining the adaptation zone as well as the growth stage of agricultural practices and responses to heat accumulation. The conclusion of the current study is expected to be widely adopted by soybean regionalization and germplasm exchanges throughout the world.
Role of Community Gardens in Development of Housing Estates in Polish Cities
Authors: Janowska Beata , Łój Jagoda , Andrzejak Roman
Published: June 2022

The amount of green is relatively low in most cities. Often, the cause of this situation is urban policy which favours developers. Far-reaching urbanization results in various environmental and social problems. In those circumstances, a need for the wider revitalization and reorganization of urban space becomes even more important and necessary. Our article is a review. It discusses the significance of community gardens in Poland due to people’s limited access to private gardens and the growing development of housing estates in cities. In recent decades, cities have developed rapidly in Poland. However, this development was often uncontrolled because very compact building patterns were applied in new housing estates. As a result, in many places the urban tissue lacked common public space. Urban planners adopted a new approach to public goods, including green spaces, and reconsidered the development of cities. Common space was found to be fundamental for the development of the identity of individual neighbourhood groups and, consequently, for social changes. Community gardens are an example of changes introduced to the urban landscape as a consequence of considering common space significant. As a result, local residents can integrate and change their attitude to public space, which is perceived as part of the community. Users gain a sense of community, solidarity, and shared responsibility. The community garden becomes a reason for neighbourhood residents to integrate despite the fact that they may differ in their political views, background, and age. Community gardens are established in a similar way all over the world. However, there might be local differences in the legal system, people’s mentality, and access to land. Community gardens are a new phenomenon in Poland. They are established mainly in big cities, e.g., in Warsaw, Kraków, and Poznań. Community gardens have numerous advantages. Their users are physically active, and they are thus at lower risk of chronic and non-infectious diseases. They grow vegetables and thus change their eating habits. In addition, community gardens reduce stress. Due to gardening, users feel better, broaden their social contacts, participate in cultural events, and enrich their diet. This article shows how the intensive development of housing estates in Poland affects the availability of green areas. The advantages of community gardens in Poland and other countries, methods of plant cultivation and how community gardens functioned during the COVID-19 pandemic are discussed. With this short review, we want to broaden the knowledge about community gardens as their development in Poland is different than in other countries and to encourage their establishment due to the decreasing amount of green space and dense urban development.
Evaluation of the Effect of Different Co-Solvent Mixtures on the Supercritical CO2 Extraction of the Phenolic Compounds Present in Moringa oleifera Lam. Leaves
Authors: Yerena-Prieto Beatriz Juliana , Gonzalez-Gonzalez Monserrat , García-Alvarado Miguel Ángel , Casas Lourdes , Palma Miguel , Rodríguez-Jimenes Guadalupe del Carmen , Barbero Gerardo F. , Cejudo-Bastante Cristina
Published: June 2022

Supercritical fluid extraction (SFE), using CO2, is a novel, sustainable and very efficient technique for the recovery of highly apolar compounds. However, the recovery of phenolic compounds requires the use of different co-solvent combinations such as water and ethanol to enhance the recovery of these compounds through the optimization of a number of variables. In this sense, the effect of pressure (100, 150 and 200 bar), temperature (50, 65 and 80 °C), extraction time (30, 60, 90, 120, 150 and 180 min) and the effect of the different percentages of ethanol and water as co-solvents on the composition and phenolic content of moringa leaf extracts were evaluated. Six major flavonoids were identified by ultra-high-performance liquid chromatography coupled to a quadrupole-time-of-flight mass spectrometer (UHPLC-Q-ToF-MS). Pressure and temperature had a significant effect on the phenolic composition of the extracts, as well as on their concentrations. The highest concentration of total flavonoids compounds (TFCs) was obtained by using a mixture of CO2 and water of 50:50 (v/v) at 100 bar, at 65 °C after a 120 min extraction time that produced a concentration of 11.66 mg ± 0.02 mg TFC g−1 sample, which corresponds to 89.0% of the total flavonoids of the sample, obtained by exhaustive extraction.
Hyperspectral Sensing of Plant Diseases: Principle and Methods
Authors: Wan Long , Li Hui , Li Chengsong , Wang Aichen , Yang Yuheng , Wang Pei
Published: June 2022

Pathogen infection has greatly reduced crop production. As the symptoms of diseases usually appear when the plants are infected severely, rapid identification approaches are required to monitor plant diseases at early the infection stage and optimize control strategies. Hyperspectral imaging, as a fast and nondestructive sensing technology, has achieved remarkable results in plant disease identification. Various models have been developed for disease identification in different plants such as arable crops, vegetables, fruit trees, etc. In these models, important algorithms, such as the vegetation index and machine learning classification and methods have played significant roles in the detection and early warning of disease. In this paper, the principle of hyperspectral imaging technology and common spectral characteristics of plant disease symptoms are discussed. We reviewed the impact mechanism of pathogen infection on the photo response and spectrum features of the plants, the data processing tools and algorithms of the hyperspectral information of pathogen-infected plants, and the application prospect of hyperspectral imaging technology for the identification of plant diseases.
Impact of Combined Drought and Heat Stress and Nitrogen on Winter Wheat Productivity and End-Use Quality
Authors: Statkevičiūtė Gražina , Liatukas Žilvinas , Cesevičienė Jurgita , Jaškūnė Kristina , Armonienė Rita , Kuktaite Ramune , Brazauskas Gintaras
Published: June 2022

Water deficit and heat stress are the main abiotic stresses affecting the yield and quality of winter wheat. The increasing frequency of the simultaneous occurrence of these two stresses might threaten global food security and drives the need to breed resilient high-quality cultivars. The aim of this study was to evaluate the grain yield, quality and gluten protein characteristics in 50 winter wheat cultivars and breeding lines during the harvest years of 2018 and 2019. The yield and grain quality components were affected more severely by the combined heat and drought in 2019 than the drought in 2018. Two nitrogen (N) fertilization regimes were studied, sustainable (S, 15/100/30 kg N ha−1) and high-input (HI, 15/100/100 kg N ha−1). The yield was higher in HI trials compared to S trials by 2.2 t ha−1 in 2018 and by 2.4 t ha−1 in 2019. Higher protein content and sedimentation volume and lower yield, test weight and starch content were observed under combined heat and drought stress in 2019 compared to 2018. Genotypes containing the Glu-D1 x5-y10 allele exhibited the higher amounts of unextractable polymeric proteins (%UPP = 58.5%) in gluten studied by size exclusion liquid chromatography (SE-HPLC) as compared to Glu-D1 x2-y12 allele (%UPP = 54.3%). Genotype was the main determinant of gluten protein characteristics regardless of the nitrogen application and the abiotic stress conditions. The results suggest that the relatively mild drought and heat events in Lithuania might not threaten gluten quality in the future; however, breeding efforts should be directed towards improved drought and heat stress resistance to ensure stable wheat productivity in the region.
Effect of Wheat Monoculture on Durum Wheat Yield under Rainfed Sub-Humid Mediterranean Climate of Tunisia
Authors: Bouatrous Asma , Harbaoui Kalthoum , Karmous Chahine , Gargouri Samia , Souissi Amir , Belguesmi Karima , Cheikh Mhamed Hatem , Gharbi Mohamed Salah , Annabi Mohamed
Published: June 2022

Cultivating cereals in monoculture systems contributes to the decrease in grain yield and quality. Currently, under Mediterranean climate conditions of Tunisia, wheat mono-cropping covers more than 70% of cereal areas. In order to reveal the impact of this practice on cereal productivity, five improved durum wheat cultivars (Karim, Khiar, Om Rabiaa, Razzek, and Maali) were conducted under two conditions of previous wheat crop: one-year wheat previous crop (W) and two successive years (W-W). Then, they were assessed for grain yield (GY), yield components (NKS, TKW, NS), straw yield, harvest index (SY, HI), and grain quality parameters during three consecutive cropping seasons (2017, 2018, and 2019). The results showed significant effects of cropping season for all measured parameters, except thousand kernel weight (TKW). A significant effect (p < 0.05) of Pre-Crop was observed on yield components. However, grain yield (GY) was improved after one-year wheat Pre-Crop (W) (4082.3 kg ha−1) more than after two years (W-W) (3277.3 kg ha−1). Our results show that, based on the three-year experiment, almost all yield related traits were significantly affected by the genotype except HI and NS. The highest GYs were recorded for Om Rabiaa (4010.4 kg ha−1) and Nasr (3765.76 kg ha−1). All grain quality was significantly (p < 0.05) affected by cropping season, but only gluten content (GC) and vitreousness aspect (Vit A) were affected by genotype. On the other hand, the Pre-Crop W-W decreased grain protein concentration (GPC) (12.13%) and GC (22.14%) but no significant effect was observed on the Vit A of grain in our study. Furthermore, GY was positively correlated with HI (r = 0.64), NKS (r = 0.59), SN (r = 0.49), GPC (r = 0.23), and GC (r = 0.23). According to stability analysis, the Karim cultivar is the most stable genotype in wheat mono-cropping for GY and straw yield (SY). Altogether, this study provides useful information for farmers on how to produce a satisfactory yield for durum wheat cultivation under mono-cropping wheat conditions in the sub-humid environment of the Mediterranean climate of Tunisia.
Development of a Slow Loris Computer Vision Detection Model
Authors: Lei Yujie , Xiang Ying , Zhu Yuhui , Guan Yan , Zhang Yu , Yang Xiao , Yao Xiaoli , Li Tingxuan , Xie Meng , Mu Jiong , Ni Qingyong
Published: June 2022

The slow loris (Genus Nycticebus) is a group of small, nocturnal and venomous primates with a distinctive locomotion mode. The detection of slow loris plays an important role in the subsequent individual identification and behavioral recognition and thus contributes to formulating targeted conservation strategies, particularly in reintroduction and post-release monitoring. However, fewer studies have been conducted on efficient and accurate detection methods of this endangered taxa. The traditional methods to detect the slow loris involve long-term observation or watching surveillance video repeatedly, which would involve manpower and be time consuming. Because humans cannot maintain a high degree of attention for a long time, they are also prone to making missed detections or false detections. Due to these observational challenges, using computer vision to detect slow loris presence and activity is desirable. This article establishes a novel target detection dataset based on monitoring videos of captive Bengal slow loris (N. bengalensis) from the wildlife rescue centers in Xishuangbanna and Pu’er, Yunnan, China. The dataset is used to test two improvement schemes based on the YOLOv5 network: (1) YOLOv5-CBAM + TC, the attention mechanism and deconvolution are introduced; (2) YOLOv5-SD, the small object detection layer is added. The results demonstrate that the YOLOv5-CBAM + TC effectively improves the detection effect. At the cost of increasing the model size by 0.6 MB, the precision rate, the recall rate and the mean average precision (mAP) are increased by 2.9%, 3.7% and 3.5%, respectively. The YOLOv5-CBAM + TC model can be used as an effective method to detect individual slow loris in a captive environment, which helps to realize slow loris face and posture recognition based on computer vision.
Influence of Spinal Shock on the Neurorehabilitation of ANNPE Dogs
Authors: Gouveia Débora , Cardoso Ana , Carvalho Carla , Gonçalves Ana Rita , Gamboa Óscar , Canejo-Teixeira Rute , Ferreira António , Martins Ângela
Published: June 2022

Acute noncompressive nucleus pulposus extrusion (ANNPE) is related to contusive spinal cord injuries, and dogs usually appear to be exercising vigorously at the time of onset. ANNPE has a characteristic peracute onset of clinical signs during exercise or following trauma, with non-progressive signs during the first 24 h and possibly signs of spinal shock. The main aim was to assess if the presence of spinal shock affects the neurorehabilitation outcomes of ANNPE dogs. This prospective controlled cohort clinical study was conducted at the Arrábida Rehabilitation Center. All of the dogs had T3–L3 injuries and were paraplegic/monoplegic with/without nociception, the study group (n = 14) included dogs with ANNPE spinal shock dogs, and the control group (n = 19) included ANNPE dogs without spinal shock. The study group was also evaluated using a new scale—the Spinal Shock Scale (SSS)—and both groups were under the same intensive neurorehabilitation protocol. Spinal shock was a negative factor for a successful outcome within less time. SSS scores > 4 required additional hospitalization days. The protocol was safe, tolerable, and feasible and accomplished 32% ambulation within 7 days, 29% in 14 days, and 29% in 30 days. The results were better than those obtained in previous studies—94% at 60 days—and 75% of the dogs without nociception recovered ambulation. Long-term follows-ups carried out 4 years later revealed a positive evolution.
Effect of Dietary Supplementation of Bacillus subtilis on Growth Performance, Organ Weight, Digestive Enzyme Activities, and Serum Biochemical Indices in Broiler
Authors: Mohamed Taha M. , Sun Weizhong , Bumbie Gifty Z. , Dosoky Waleed M. , Rao Zebin , Hu Ping , Wu Liuting , Tang Zhiru
Published: June 2022

This study was conducted to investigate the effects of supplementing Bacillus subtilis and an antibiotic (Zinc bacitracin) in the diet of broilers on growth performance, organ weight, blood metabolites, and digestive enzymes of broiler chickens. A total of 600 1-d Arbor Acres broilers were randomly allotted to five treatments. Each treatment consisted of six replicates with four pens, and each pen had five birds. The chicks were fed (1) the basal diet (control), (2) the basal diet with 500 mg/kg Zinc bacitracin (APZ), (3) the basal diet with B. subtilis at 1 × 108 CFU/g (B.Sut-1), (4) the basal diet with B. subtilis at 3 × 108 CFU/g (B.Sut-3), and (5) the basal diet with B. subtilis at 5 × 108 CFU/g (B.Sut-5). The experiment lasted for 42 days. In this study, the supplementation of diets with B. subtilis (B.Sut-3 and B.Sut-5 groups) increased body weight gain from 1 to 21 days compared with control (p < 0.05). Additionally, the B.Sut-3 group had a significantly heavier bursa of Fabricius than control at 21 days (p < 0.05). Serum total protein, albumin, and high-density lipoprotein concentrations were increased in B.Sut-5 and APZ groups (p < 0.05) over the whole period. Serum low-density lipoprotein, very low-density lipoprotein, triglyceride, and total cholesterol concentrations were decreased in B.Sut-5 and APZ groups at 21 and 42 days (p < 0.05). Chicks in the B.Sut-5 and APZ groups had higher serum lipase, pepsin, and amylase activities (p < 0.05) at 21 and 42 days. From the results obtained from the study, it can be concluded that Bacillus subtilis ATCC19659 at 5 × 108 CFU/g could be applied as an alternative to antibiotics in poultry diets.
Influence of Elective Cesarean Calving (with and without Dexamethasone Induction) on the Erythrogram and Iron Serum Profiles in Nellore Calves
Authors: Silva Luan Ricci , Paiano Renan Braga , Diogo Mariana Guimarães de Oliveira , Yasuoka Melina Marie , Birali Ana Claúdia , Massuda Mayara Berto , Celestino Maria Luiza Kuhne , Birgel Daniela Becker , Marchese Flávio José Minieri , Neto Paulo Fantinato , Storillo Vanessa Martins , Birgel Junior Eduardo Harry
Published: June 2022

The aim of the present study is to evaluate the erythrogram and iron serum profiles of neonatal calves born spontaneously or born by elective cesarean section with or without dexamethasone induction. The research was performed on 38 newborn Nellore calves. Three groups of calves were assigned according to the type of birth: calves born by spontaneous vaginal calving (n = 10), calves born by elective cesarean section without inducing labor (n = 14), and calves born by elective cesarean section with labor induction with dexamethasone (n = 14). Blood samples to assess red blood cell count (RBC), hemoglobin, hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), concentration of mean corpuscular hemoglobin (MCHC), serum iron (SFe), total capacity to bind iron to transferrin (TIBIC), and transferrin saturation index (TSI) were performed at calving (0, 3, 6, and 12 h of life) and on 1, 2, 3, 5, 7, 10, 15, and 30 days of life. Regardless of the experimental group (calves born spontaneously, or born by elective cesarean section with or without dexamethasone induction), in the first day of life there was a decrease in the number of red blood cells, hemoglobin rates, and values of the globular volume. In the period of the first 10 days of life, animals from spontaneous vaginal delivery quickly recovered values of erythrocytes, hemoglobin, and packed cell volume, whereas animals born by elective C-section (induced and uninduced) did not recover as quickly in their rates of hemoglobin and packed cell volume values. In calves born by elective C-section (induced and uninduced), it was observed in their period between 10 and 30 days of life that the MCV and MCH were reduced by passing the presenting microcytic hypochromic when compared with calves obtained by spontaneous vaginal delivery. In the period between 10 and 30 days of life, the levels of SFe and TSI in animals born by elective C-section (induced and uninduced) are significantly lower. The differences in the erythrogram values between Nellore calves born spontaneously and those by elective C-section with or without induction must be considered consequent to the process of neonatal adaptation to extrauterine life. Iron supplementation in the first month of life in calves from cesarean could be recommended to prevent anemia of this iron deficiency.
Association between Head-to-Chest Circumference Ratio and Intrauterine Growth-Retardation Related Outcomes during Preweaning and Postweaning
Authors: Manriquez Diego , Poudevigne Guilhem , Roche Etienne , Waret-Szkuta Agnes
Published: June 2022

The objective of this study is to evaluate the association between the head-to-chest circumference ratio (HCR) and birth weight (BW), colostrum intake, and average daily weight gain (ADG) at preweaning and postweaning periods. Additionally, associations between HCR and PCV-2 serum antibody titers and the PCV-2 seroconversion ratio (SCR) were assessed. Head and chest circumferences were measured at birth, and HCR was calculated from 110 piglets born from 8 pregnant sows randomly selected from maternity pens. Linear mixed models were used to test whether changes in HCR were associated with fluctuations of BW, colostrum intake, and ADG. In addition, HCR least-square means were compared between piglets classified as lower or greater BW, colostrum intake, and ADG. Finally, receiving operating characteristic curve analyses were performed to estimate HCR thresholds for discriminating between lower and greater performance piglets during preweaning and postweaning periods. Increments in HCR were associated with lower BW, colostrum intake, and ADG. An HCR threshold of 0.82 maximized sensibility and specificity for the classification of lower and greater performance piglets regarding BW, colostrum intake, and ADG during the periods of 0 to 7 and 0 to 69 days of life. When piglets were categorized into HCR ≤ 0.82 and HCR > 0.82 groups, piglets with HCR ≤ 0.82 had lower (log10) PCV-2 serum antibody titers at 26 days of life compared with piglets with HCR > 0.82 (3.30 ± 0.05 vs. 3.47 ± 0.05 g/dL). On the other hand, piglets that showed low SCR between 26 and 69 days of life had greater HCRs compared with piglets with high SCRs (0.83 ± 0.008 vs. 0.8 ± 0.008). The use of HCRs allowed us to identify piglets with lower performance and impaired immune response against PCV-2. The HCR indicator could be used as a selection criterion for preventive management for piglets showing delayed performance potentially associated with IUGR.
The Alimentary Tract of African Bony-Tongue, Heterotis niloticus (Cuvier, 1829): Morphology Study
Authors: Guerrera Maria Cristina , Aragona Marialuisa , Briglia Marilena , Porcino Caterina , Mhalhel Kamel , Cometa Marzio , Abbate Francesco , Montalbano Giuseppe , Laurà Rosaria , Levanti Maria , Germanà Germana , Zaccone Giacomo , Zuwala Krystyna , Kuciel Michal , Germanà Antonino
Published: June 2022

A morphological study of the alimentary tract, from the oropharyngeal cavity to the rectum, including the attached glands, of African bony-tongue, Heterotis niloticus (Cuvier, 1829) was carried out by gross anatomy, and light microscope analysis. This study aimed to give a deeper knowledge of the alimentary tract morphological features of this species of commercial interest. H. niloticus is distinguished by individual morphological characteristics showing a digestive tract similar to that of reptiles and birds. Within the oropharyngeal cavity, two tubular structures with digitiform ends are arranged on both lateral sides of the triangular tongue. The oropharyngeal cavity connects the stomach by a short esophagus. This latter is adapted to mechanical trituration, and it is divided into a pars glandularis and a thick-walled pars muscularis. The gizzard flows into the anterior intestine and two blind pyloric appendages, which exhibit specific functions, including immune defense for the presence of secondary lymphoid organs. The anterior intestine continues with the middle and posterior tracts up into the rectum. According to the histological observations, all regions of the alimentary tract have common structural features, typical of hollow organs, with differences in the mucosa structure that reflects the different functions of the apparatus, from mouth to anus. Within this study, we provided the first basis for future studies on optimizing rearing conditions, feed conversion ratio, and the digestive capacity, improving the growth performance of this species, and ensuring its conservation.
Association of Serum Glucose, Serotonin, Aspartate Aminotransferase, and Calcium Levels with Meat Quality and Palatability Characteristics of Broiler Pectoralis Major Muscle
Authors: Lee Boin , Choi Young Min
Published: June 2022

This study investigated the correlations between apoptosis-related blood biochemical parameters measured at exsanguination and the meat and sensory quality characteristics of broiler pectoralis major muscle. The concentration of serotonin showed a positive correlation with aspartate aminotransferase (AST) activity (p < 0.001) and a negative correlation with calcium content (p < 0.01). All serum parameters showed relationships with muscle pH at 15 min and/or 24 h postmortem (p < 0.05). Serum AST activity, which had a negative correlation with calcium content (p < 0.01), was positively related with muscle pH and negatively correlated with Warner–Bratzler shear force values (WBS, p < 0.05). Principal component analysis results revealed the associations between AST activity and meat quality traits, including pH24h, lightness, and WBS. Furthermore, cooked breast with higher AST activity and lower calcium level tended to exhibit higher scores of tenderness and overall acceptability than that with lower AST activity and higher calcium level (p < 0.05).
Serum Insulin-like Growth Factor-1 Is a Biomarker of Testosterone Production and Intact Acrosome in Asian Elephants (Elephas maximus)
Authors: Yang Yuqing , Suwimonteerabutr Junpen , Angkawanish Taweepoke , Chatdarong Kaywalee
Published: June 2022

The objective of this study was to find relationships among serum IGF-1, serum testosterone, seminal plasma IGF-1 concentrations and semen parameters in Asian elephants (Elephas maximus). A total of 17 ejaculates (one to three ejaculates/bull) were collected from seven captive elephant bulls by performing rectal massage. Before each ejaculation, blood samples were obtained for serum IGF-1 and testosterone assays. Subsequently, the semen characteristics of each ejaculate were evaluated. Mean serum IGF-1 concentration of elephant bulls was estimated as 326.3 ± 114.6 ng/mL (median, 286.2 ng/mL; range, 167.4–542.7 ng/mL). An increase in serum IGF-1 concentration was found to correlate with the percentage of spermatozoa with intact acrosomes. In addition, IGF-1 concentration was positively correlated with testosterone level. However, seminal IGF-1 concentrations could not be detected. In conclusion, our findings suggest that serum IGF-1 concentration is likely a biomarker of normal testicular functions, particularly spermatogenesis in elephants. Moreover, this commercial IGF-1 ELISA is eligible for analyzing serum IGF-1 concentration in Asian elephants.
Measurement of Tetrahydrobiopterin in Animal Tissue Samples by HPLC with Electrochemical Detection—Protocol Optimization and Pitfalls
Authors: Vujacic-Mirski Ksenija , Oelze Matthias , Kuntic Ivana , Kuntic Marin , Kalinovic Sanela , Li Huige , Zielonka Jacek , Münzel Thomas , Daiber Andreas
Published: June 2022

Tetrahydrobiopterin (BH4) is an essential cofactor of all nitric oxide synthase isoforms, thus determination of BH4 levels can provide important mechanistic insight into diseases. We established a protocol for high-performance liquid chromatography/electrochemical detection (HPLC/ECD)-based determination of BH4 in tissue samples. We first determined the optimal storage and work-up conditions for authentic BH4 and its oxidation product dihydrobiopterin (BH2) under various conditions (pH, temperature, presence of antioxidants, metal chelators, and storage time). We then applied optimized protocols for detection of BH4 in tissues of septic (induced by lipopolysaccharide [LPS]) rats. BH4 standards in HCl are stabilized by addition of 1,4-dithioerythritol (DTE) and diethylenetriaminepentaacetic acid (DTPA), while HCl was sufficient for BH2 standard stabilization. Overnight storage of BH4 standard solutions at room temperature in HCl without antioxidants caused complete loss of BH4 and the formation of BH2. We further optimized the protocol to separate ascorbate and the BH4 tissue sample and found a significant increase in BH4 in the heart and kidney as well as higher BH4 levels by trend in the brain of septic rats compared to control rats. These findings correspond to reports on augmented nitric oxide and BH4 levels in both animals and patients with septic shock.
Effect of Low-Dose Radiotherapy on the Circulating Levels of Paraoxonase-1-Related Variables and Markers of Inflammation in Patients with COVID-19 Pneumonia
Authors: Rodríguez-Tomàs Elisabet , Acosta Johana C. , Torres-Royo Laura , De Febrer Gabriel , Baiges-Gaya Gerard , Castañé Helena , Jiménez Andrea , Vasco Carlos , Araguas Pablo , Gómez Junior , Malave Bárbara , Árquez Miguel , Calderón David , Piqué Berta , Algara Manel , Montero Ángel , Simó Josep M. , Gabaldó-Barrios Xavier , Sabater Sebastià , Camps Jordi , Joven Jorge , Arenas Meritxell
Published: June 2022

The aim of our study was to investigate the changes produced by low-dose radiotherapy (LDRT) in the circulating levels of the antioxidant enzyme paraoxonase-1 (PON1) and inflammatory markers in patients with COVID-19 pneumonia treated with LDRT and their interactions with clinical and radiological changes. Data were collected from the IPACOVID prospective clinical trial (NCT04380818). The study included 30 patients treated with a whole-lung dose of 0.5 Gy. Clinical follow-up, as well as PON1-related variables, cytokines, and radiological parameters were analyzed before LDRT, at 24 h, and 1 week after treatment. Twenty-five patients (83.3%) survived 1 week after LDRT. Respiratory function and radiological images improved in survivors. Twenty-four hours after LDRT, PON1 concentration significantly decreased, while transforming growth factor beta 1 (TGF-β1) increased with respect to baseline. One week after LDRT, patients had increased PON1 activities and lower PON1 and TGF-β1 concentrations compared with 24 h after LDRT, PON1 specific activity increased, lactate dehydrogenase (LDH), and C-reactive protein (CRP) decreased, and CD4+ and CD8+ cells increased after one week. Our results highlight the benefit of LDRT in patients with COVID-19 pneumonia and it might be mediated, at least in part, by an increase in serum PON1 activity at one week and an increase in TGF-β1 concentrations at 24 h.
Dietary Parsley Seed Mitigates Methomyl-Induced Impaired Growth Performance, Hemato-Immune Suppression, Oxidative Stress, Hepato-Renal Damage, and Pseudomonas aeruginosa Susceptibility in Oreochromis niloticus
Authors: El-Houseiny Walaa , Algharib Samah Attia , Mohamed Eman A. A. , Metwally Mohamed M. M. , Mahmoud Yasmina K. , Alghamdi Youssef S. , Soliman Mohamed Mohamed , Abd-Elhakim Yasmina M. , El-Murr Abd Elhakeem
Published: June 2022

The present experiment investigated the potential protective role of parsley (Petroselinum crispum) seed meal (PSM) in alleviating methomyl (MET)-adverse impacts on growth, whole-body composition, hematological indicators, hepatorenal function, immune response, oxidative status, and disease resistance to Pseudomonas aeruginosa. For this purpose, 225 healthy Nile tilapia (Oreochromis niloticus) were allotted into five groups (45 fish/group in triplicate). One group was reared in clean water and fed a non-supplemented basal diet, while the other groups were exposed to 20.39 μg L−1 MET and fed a non-fortified basal diet or basal diets supplemented with 0.5, 1.0, or 2.0% of PSM for 60 days. The obtained data revealed significantly lower weight gain, feed intake, and specific growth rate, but higher feed conversion ratio and decreases in crude protein, lipid, and ash contents in the MET-exposed fish. Anemia, leukopenia, lymphocytopenia, and esonipenia were also obvious. Furthermore, MET-exposed fish had significantly higher serum levels of hepatic enzymes and renal damage products. Nevertheless, there was a significant depletion of enzymatic and non-enzymatic antioxidants and increased malondialdehyde, myeloperoxidase, and tumor necrosis factor-α levels in MET-exposed fish. The MET exposure significantly depressed lysozyme activity, nitric oxide, complement3, acetylcholinesterase activity, total proteins, globulin, and albumin levels in O. niloticus serum. Furthermore, pathological alterations in the liver and kidney were noted. The relative percentage of survival rate in MET-exposed fish was dramatically reduced on day 14 post-challenge with P. aeruginosa. The inclusion of PSM, on the other hand, greatly alleviated most of the MET-related negative effects. Taken together, the dietary intervention with PSM has a promising role in alleviating MET-deleterious impacts, rendering parsley seeds a viable aqua feed additive for O. niloticus.
Liquid Chromatography–High-Resolution Mass Spectrometry (LC-HRMS) Profiling of Commercial Enocianina and Evaluation of Their Antioxidant and Anti-Inflammatory Activity
Authors: Della Vedova Larissa , Ferrario Giulio , Gado Francesca , Altomare Alessandra , Carini Marina , Morazzoni Paolo , Aldini Giancarlo , Baron Giovanna
Published: June 2022

Enocianina is an anthocyanin-rich extract obtained from grape pomace. It is widely used as a colorant in the food industry and, in addition to anthocyanins, it also contains a variety of polyphenols. To understand whether enocianina, besides its coloring effect, may offer potential health benefit applications, we aimed to fully characterize the profile of four commercial enocianinas and assess their radical scavenging, enzymatic, antioxidant, and anti-inflammatory activities. LC-ESI-MS/MS analysis identified 90 phytochemicals. The relative content of each anthocyanin was assessed by a semi-quantitative analysis, with malvidin derivatives being the most abundant. UV-VIS spectroscopy detected total amounts of polyphenols and anthocyanins of 23% and 3.24%, respectively, indicating that anthocyanins represent a minor fraction of total polyphenols. Multiple linear regression analysis indicated that the radical scavenging activity is related to the total polyphenol content and not to anthocyanins. All four enocianinas dose-dependently activate Nrf2, and such activity was correlated with catechol-containing polyphenol content. Finally, all enocianinas showed dose-dependent anti-inflammatory activity, which at the highest concentrations tested was closely related to the total polyphenol content and was explained by radical scavenging, Nrf2 activation, and other mechanisms related to the polyphenolic components.
Influence of Quality of Mounting Process of RF Transistors on Their Thermal Parameters and Lifetime
Authors: Górecki Krzysztof , Kowalke Wojciech , Ptak Przemysław
Published: June 2022

The paper presents the results of investigations illustrating the influence of imperfections in the soldering process on thermal properties of transistors operating in RF (Radio Frequency) systems. The methods of measurements used to determine the junction and case temperature of the tested transistors and their thermal resistance are described. The results of the performed measurements are presented and discussed, and their statistical analysis is carried out. The correlation between thermal parameters of the tested transistors, parameters of the assembly process and the measurement conditions is investigated. Some calculation results illustrating an influence of device thermal resistance and dissipated power on changing its lifetime are shown and discussed. It is also shown that no correlation between the void sizes and the device thermal resistance and lifetime is observed. Possibilities of improving the assembly process are indicated, which allows extending the expected lifetime of the tested transistors.
Roots of Lithospermum erythrorhizon Alleviated Ovalbumin-Induced Allergic Rhinitis and IgE-triggered Degranulation of RBL-2H3 Cells
Authors: Kang Tae Kyeom , Le Tam Thi , Choi Su-Young , Song Hee-Won , Lee Wook-Bin , Jung Sang Hoon
Published: June 2022

Lithospermum erythrorhizon (L. erythrorhizon) root is used in traditional medicine for its anti-inflammatory, antibacterial, and antioxidant properties. However, no studies have examined its impact on allergic rhinitis (AR). Here, we explored the protective effects of L. erythrorhizon in immunoglobulin E (IgE)-stimulated RBL-2H3 cells and in an ovalbumin (OVA)-induced AR mouse model. In the latter, we examined nasal mucosal inflammation, allergen-specific cytokine production, and histological changes to the nasal mucosa. In the mouse model, oral administration of an ethanol extract of L. erythrorhizon (LE) led to a marked reduction in rubbing and sneeze frequency, a significant decrease in serum OVA-specific IgE and IgG1 levels, and a significant increase in the IgG2a/IgG1 ratio. LE also reduced expression of interleukin (IL)-4, IL-5, and IL-13 in nasal lavage fluid (NALF), and suppressed inflammatory cell infiltration and epithelial degradation in nasal tissues. In IgE-stimulated RBL-2H3 cells, LE suppressed release of degranulation markers such as β-hexosaminidase and histamine. Based on these findings, we suggest that LE may ameliorate OVA-induced AR by regulating mast cell-mediated inflammatory responses.
Kinematic Chain Equivalent Method for Tube Model and Elastodynamic Optimization for Parallel Mechanism Based on Matrix Structural Analysis
Authors: Zhou Caizhi , Han Youcheng , Guo Weizhong
Published: June 2022

The platforms of parallel mechanisms usually suffer vibration loads. In these cases, structure elastodynamic analysis and elastodynamic optimization of parallel mechanisms are important. A tube structure is very common for parallel mechanisms. This work establishes the model of a tube structure based on matrix structural analysis. The kinematic pair equivalent method is used to simulate the surface contact between the inner and outer tubes. The corresponding mass and stiffness matrices are derived through the strain energy minimization method. The reconfigurable legged lunar lander has been used as an example to verify the effectiveness of this method. By adding the mechanism configuration to the optimization process, the equivalent static load method and the desirability approach are combined and modified. A procedure for the multi-objective elastodynamic optimization of parallel mechanisms is proposed. The optimization procedure is implemented on the lander and the results show a reduction in mass and an increase in natural frequency.
GPU-Accelerated Target Strength Prediction Based on Multiresolution Shooting and Bouncing Ray Method
Authors: Zhao Gang , Sun Naiwei , Shen Shen , Wu Xianyun , Wang Li
Published: June 2022

The application of the traditional planar acoustics method is limited due to the low accuracy when computing the echo characteristics of underwater targets. Based on the concept of the shooting and bouncing ray which considers multiple reflections on the basic of the geometrics optics principle, this paper presents a more efficient GPU-accelerated multiresolution grid algorithm in the shooting and bouncing ray method (SBR) to quickly predict the target strength value of complex underwater targets. The procedure of the virtual aperture plane generation, ray tracing, scattered sound field integral and subdividing the divergent ray tubes are all implemented on the GPU. Particularly, stackless KD-tree traversal is adopted to effectively improve the ray-tracing efficiency. Experiments on the rigid sphere, cylinder and corner reflector model verify the accuracy of GPU-based multiresolution SBR. Besides, the GPU-based SBR is more than 750 times faster than the CPU version because of its tremendous computing capability. Further, the proposed accelerated GPU-based multiresolution SBR improves runtime performance at least 2.4 times that of the single resolution GPU-based SBR.
Renyi Joint Entropy-Based Dynamic Threshold Approach to Detect DDoS Attacks against SDN Controller with Various Traffic Rates
Authors: Aladaileh Mohammad Adnan , Anbar Mohammed , Hintaw Ahmed J. , Hasbullah Iznan H. , Bahashwan Abdullah Ahmed , Al-Sarawi Shadi
Published: June 2022

The increasing incidence of distributed denial-of-service (DDoS) attacks has made software-defined networking (SDN) more vulnerable to the depletion of controller resources. DDoS attacks prevent the SDN controller from processing all incoming data efficiently, potentially disrupting a network or denying legitimate users access to network services. Thus, the protection of the SDN controller is crucial, especially from the ones that exploit the SDN characteristics. In this paper, the authors propose an efficient detection approach for low- and high-rate DDoS attacks on the controller with a high detection rate and a low false positive rate by adapting a dynamic threshold algorithm rather than a static one and proposing a new rule-based detection mechanism. In addition, the proposed approach was evaluated using eight simulation scenarios representing all potential attacks against the SDN controller in terms of attack traffic rates (low or high), sources (either single or multiple hosts), and targets (single or multiple victims). The experiment results show that the proposed approach is more effective than the existing approaches based on attack detection and false positive rates.