bluuebunny/crossref_metadata_2025 huggingface dataset:
Pyroptosis Accelerates Spiral Ganglion Neuronal Degeneration Induced by Aminoglycosides in the Cochlea
Authors: Xing Yazhi , Fang Jia , Li Zhuangzhuang , Li Mingxian , Liu Chengqi , Zhang Xiaoxu , Li Yini , Mao Song , Shi Haosong , Su Kaiming , Yu Dongzhen , Shi Haibo
Published: December 2021

Background
In aminoglycoside-induced hearing loss, damage to spiral ganglion neurons (SGNs) accelerates gradually after the acute outer hair cell death, accompanied by macrophage infiltration and cytokine release. Pyroptosis plays a critical role in neurodegenerative diseases. Here, we explored the potential role of pyroptosis in SGN degeneration.
Methods
C57BL/6J mice were randomly divided into a kanamycin plus furosemide group and saline control group. Auditory functions were evaluated by auditory brainstem response tests conducted before treatment and at 1, 5, 15, and 30 days after treatment. HCs and SGNs were assessed for morphological alterations. SGNs were subjected to RNA sequencing and mRNA and protein analyses of NLRP3 inflammasome-related molecules. Macrophage activation was evaluated based on morphological and mRNA alterations. The effect of NLRP3 inhibition on SGN survival after kanamycin treatment was evaluated in organ explant cultures treated with Mcc950, a specific inhibitor of the NLRP3 inflammasome.
Results
Kanamycin and furosemide administration led to irreversible deterioration of the auditory brainstem response threshold, accompanied by acute loss of outer hair cells and gradually progressive loss of inner hair cells. SGNs showed a progressive decrease in quantity, as well as swelling and membrane rupture, at 15 and 30 days. RNA sequencing of SGNs showed that inflammation and immune-related responses were significantly upregulated, as was the expression of the inflammasome-related gene NLRP3. During 30 days of kanamycin exposure, the canonical pyroptosis pathway was constantly activated in SGNs. Activation and infiltration of microglia-like cells/macrophages, and increased production of cytokines, hallmarks of neuroinflammation, were also observed. Mcc950 significantly ameliorated SGN degeneration by inhibiting NLRP3 expression and promoting release of interleukins 1β and 18.
Conclusions
Pyroptosis causes cell death during aminoglycoside-induced SGN degeneration. Activation of the NLRP3 inflammasome leads to a cascade of inflammatory events in SGNs. Inhibition of the NLRP3 inflammasome significantly alleviates SGN damage, suggesting that it could serve as a new molecular target for the treatment of aminoglycoside-induced SGN degeneration.
TIGAR Enhanced Free Ca2+ Concentration in Hepatocellular Carcinoma Cells to Accelerate the Sustained Proliferation and Drug Resistance
Authors: Xie Jia-ming , Sui Yi-qun , Feng Xin-yu , Feng Zhen-yu , Li Wei , Ma Xiao-ming , Gao De-kang , Du Peng , Zhang Heng , Ye Zhen-yu , Chen Wei
Published: December 2021

Background: To study the role of TP53-induced glycolysis and apoptosis regulator (TIGAR) in hepatocellular carcinoma (HCC) and drug resistance.Methods: HCC cells (HepG2 and SMMC7721) were used in this study. Fura 2-AM was used to assess cytosolic free Ca2+ concentrations ([Ca2+]i) within the two HCC cell lines. Nimodipine (NMDP), a Ca2+ antagonist, was used to reduce cytosolic [Ca2+]i level. Proliferation of HCC was measured using cell counting kit-8 (CCK-8). The roles of TIGAR and Ca2+ in drug resistance of HCC cells were assessed using epirubicin (Epi), 5-fluorouracil (5-FU), or cisplatinum (DDP).Results: Knockdown of TIGAR significantly suppressed cell viability, reduced [Ca2+]i, restrained protein expression of Ca2+-activated cysteine proteinases (Calpain1 and 2), as well as blocked the activation of nuclear factor kappa B (NF-κB) through an increase of cytoplasmic NF-κB and reduction of nuclear NF-κB. However, overexpression of TIGAR (oeTIGAR) resulted in the opposite. Evidence also shows that oeTIGAR suppressed the sensitivity of HCC to Epi, which was retarded by NMDP as an additional treatment. TIGAR interference could enhance the sensitivity of HCCs with high TIGAR expression to drugs.Conclusions: TIGAR promoted HCC progression and induced drug resistance, and the mechanism involved was [Ca2+]i-mediated activation of Calpain 1 and 2 and NF-κB signaling.
Contextualizing adverse event following immunization data of coronavirus disease 2019 (COVID-19) vaccines in VigiAccess: An approach for reducing COVID-19 vaccine hesitancy
Authors: Yamoah Peter , Mensah Kofi Boamah , Mpoku Thelma Alalbila , Padayachee Neelaveni , Oosthuizen Frasia , Bangalee Varsha
Published: December 2021

IntroductionVaccination features high among the public health interventions that have contributed significantly to global health. Following the March 2020 declaration by the World Health Organization that coronavirus 2019 (COVID-19) is a global pandemic, several vaccines have been developed and administered to curb the spread of COVID-19. One of the threats to attaining adequate vaccination uptake for these relatively new vaccines are concerns people have about the adverse event following immunization (AEFI) information. This study sought to assess AEFIs reported on COVID-19 vaccines approved for use so far in VigiAccess and to make a case for why AEFIs data in the database must be interpreted with caution.MethodsThe study followed a cross-sectional quantitative study design. VigiAccess was searched on November 10, 2021 for AEFIs reported so far for all the 12 approved COVID-19 vaccines. Data were captured among age groups, sex and continents of the world. Descriptive data were summarized using tables. Frequencies and percentages were used to categorize descriptive variables. No ethical approval was obtained before the commencement of the study as this was essentially a secondary data analysis of AEFI reports which cannot be linked to any individual. Consequently, there was no need for the informed consent process.ResultsOverall, 2,457,386 AEFIs had been reported in VigiAccess. AEFIs were found to be highest among the 18-44 age group (39.7%) and lowest in vaccine recipients below 12 years (0.1%). AEFIs were more common in females than male vaccine recipients with over two-thirds of the vaccine recipients being females. Among the continents of the world, AEFI reports were highest for Europe (50%) and lowest for Africa (3%). The top 10 commonly reported AEFI types were as follows: general disorders and vaccine administrative site conditions (1,481,549, 60.1%), nervous system disorders (1,046,928, 42.6%), musculoskeletal and connective tissue disorders (704,657, 28.6%), gastrointestinal disorders (495,997, 20.2%), investigations with undesirable outcomes (341,677, 13.9%), skin and subcutaneous tissue disorders (335,932, 13.6%), respiratory, thoracic and mediastinal disorders (262,158, 10.6%), infections and infestations (180,873, 7.3%), vascular disorders (132,533, 5.3%) and injury, poisoning and procedural complications (122,519, 5%).ConclusionThe study showed that over 2 million COVID-19 AEFIs were spontaneously reported in VigiAccess, however, no causal relationships could be established between the vaccines and the AEFIs. The public accessing VigiAccess data should be made aware of this lack of association so that they may make well informed health decisions.
In Vitro Synergistic Antimicrobial Activity of Combinations of Meropenem, Colistin, Tigecycline, Rifampin, and Ceftolozane/Tazobactam Against Carbapenem-Resistant Acinetobacter Baumannii
Authors: Ju Yong Guk , Lee Hak Joon , Yim Hong Soon , Lee Chang Kyu , Lee Mingoo , Sohn Jang Wook , Yoon Young Kyung
Published: December 2021

The aim of this study was to investigate the in vitro activity of various antimicrobial combinations against carbapenem-resistant Acinetobacter baumannii (CRAB) isolates producing OXA-23 carbapenemases.In vitro activity of six two-drug combinations against CRAB isolates collected from patients with CRAB bacteremia was evaluated using the checkerboard method and time-kill assay [0.5 ×, 1 ×, 2 × minimum inhibitory concentrations (MIC)], to identify potential synergistic and bactericidal two-drug combinations against CRAB isolates, using meropenem, colistin, tigecycline, rifampin, and ceftolozane/tazobactam. All 10 CRAB isolates in our study carried the OXA-58-type and OXA-23-type carbapenem-hydrolyzing oxacillinase. The colistin-ceftolozane/tazobactam combination demonstrated a synergistic effect in both the time-kill assay (using an antibiotic concentration of 1 × MIC) and the checkerboard method, while simultaneously showing a bactericidal effect in the time-kill assay. For all 10 CRAB isolates, time-kill curves showed a significant synergistic bactericidal activity of the colistin-ceftolozane/tazobactam combination at 0.5 × MIC. Overall, there is substantial discordance of synergistic activity between the checkerboard microdilution and time-kill assay (with a concordance of 35%). Our study demonstrated that the two-drug combinations of colistin and ceftolozane/tazobactam can be a potential alternative for treating CRAB infections. The effect of these antibiotic combinations should be evaluated through clinical trials.
GRADEing the quality of evidence on safety: an adapted GRADE approach for preparing lists of potentially inappropriate medication for older adults
Authors: Mathes Tim , Mann Nina-Kristin , Thürmann Petra , Sönnichsen Andreas , Pieper Dawid
Published: December 2021

BackgroundSystematic reviews that synthesize safety outcomes pose challenges (e.g. rare events), which poses questions for grading the strength of the body of evidence. In this contribution, we suggest adaption of the GRADE system for grading the quality of evidence on safety outcomes for developing a potentially inappropriate medication list (PRISCUS).MethodsWe systematically assessed each of the five GRADE domains for rating-down (study limitations, imprecision, inconsistency, indirectness, publication bias) and the criteria for rating-up, considering if special considerations or revisions of the original approach were indicated. The result was gathered in a written document and discussed in a group-meeting. Subsequently, we performed a proof-of-concept application using a convenience sample of systematic reviews. Results We adapted aspects of the criteria study limitations, imprecision, publication bias and rating-up for large effect. In addition, we suggest a new criterion to account for data from subgroup-analyses. The proof-of-concept application did not reveal a need for further revision and thus we used the approach for the systematic reviews that were prepared for the PRISCUS-list. We assessed 51 outcomes for 19 clinical questions. Each of the proposed adaptions was applied. There were neither an excessive number of low and very low ratings, nor an excessive number of high ratings, but the different methodological quality of the safety outcomes appeared to be well reflected.ConclusionThe adaptions appear to have the potential to overcome some of the challenges when grading the methodological quality of harms and thus may be helpful for producers of evidence syntheses considering safety.
Transcriptomic Analysis of Camellia Sinensis (L.) Kuntze Var. Niaowangensis Q. H. Chen Leaves Under Cold Stress
Authors: Wang Ying , Wan Cheng , Li Leijia , Xiang Zhun , Wang Jihong , Li Yan , Zhao Degang
Published: December 2021

Fine varieties of the Yunwu Tribute Tea (Camellia Sinensis (L.) Kuntze var. niaowangensis Q. H. Chen) are distributed on the Yunwu Mountain, Guiding County, Guizhou province, China. Cold stress usually occurs in winter and is one of the most significant environmental factors restricting the growth of this plant as well as its geographical distribution. However, only few systematic studies have examined the molecular mechanism of cold tolerance in the Yunwu Tribute Tea. Hence, in this study, Illumina HiSeq technology was applied to investigate the cold-tolerance mechanism and for this purpose, cDNA libraries were obtained from two groups of samples namely, the cold-treated group (DW) and the control group (CK). A total of 185,973 unigenes were produced from 511,987 assembled transcripts and among these, 16,020 differentially expressed genes (DEGs) (corrected p-value <0.01, |log2(fold change)| >3), including 9,606 upregulated and 6,414 downregulated genes, were obtained. Moreover, the antioxidant enzyme system, plant hormone signal transduction, proline metabolism, tyrosine metabolism pathway, and transcription factors were analyzed and based on the results, a series of candidate genes related to cold stress were screened out and discussed. The physiological indexes related to the low temperature response were tested, along with five DEGs which were validated by quantitative real-time PCR. For this study, it is expected that the results of the transcriptome sequence of Yunwu Tribute Tea will provide valuable clues for genetic studies while helping to screen candidate genes for cold-resistance breeding in tea plants.
Audiovisual Teleconsultation For Patients With Epilepsy In Primary Care In Rural Germany: A Pilot Study On Feasibility And Acceptance
Authors: Feldmeier Gregor , Löffler Christin , Altiner Attila , Wollny Anja , von Podewils Felix , Ritzke Manuela
Published: December 2021

Background: In rural areas, epilepsy patients have limited access to specialised care. They usually have to put up with travel and waiting times of several hours. In addition, often the communication between general practitioners (GP) and specialized epileptologists about diagnosis and treatment is complicated by the high workload on both sides and the different prioritization of treatment goals. Therefore, the aim of this study was to investigate the feasibility of an interprofessional audio-visual patient-doctor teleconsultation and its acceptance in clinical practice in patients with epilepsy in a rural region in Germany.Method: Ten patients participated in telemedicine consultations in their GP practice. The practice was located in a sparsely populated region of Mecklenburg- Western Pomerania and equipped with technical equipment specifically procured for project-related purposes. An explorative qualitative interview was conducted with all participants. We based this paper on the consolidated criteria for reporting qualitative research (COREQ). Results: Despite initial uncertainties on the part of the patients regarding the consultation setting, all participants found the teleconsultation helpful. Some patients were initially intimidated and felt slightly overwhelmed by the attention provided and the technology used (multiple HD cameras, large high-resolution screens). However, during the consultation they felt supported by their GP and were satisfied that their needs were addressed in a satisfactory and timely manner. The hardware used was not felt to be a nuisance or to interfere with the conversation between doctor and patient. Patients also appreciated the time saved and the organisational convenience compared to a visit to a university outpatient clinic. Most consultations led to therapeutic consequences. Some patients seemed to benefit particularly, e.g. those who needed a change in their medication. Conclusion: This pilot study provides first evidence that teleconsultations between patients, specialists and GPs are possible in rural areas. Interprofessional collaboration between GPs and epileptologists can improve the care of patients with epilepsy. Further research should investigate the effectiveness and efficiency of interprofessional telemedicine consultations for epilepsy and other conditions.
A Novel Live Imaging-Based Assay for Visualising Species-Specific Interactions in Gametic Adhesion Molecules
Authors: Nakajima Kohdai P. , Valansi Clari , Kurihara Daisuke , Sasaki Narie , Podbilewicz Benjamin , Higashiyama Tetsuya
Published: December 2021

Successful gametic fusion requires species-specific membrane adhesion. However, the interaction of adhesion molecules in gametes is difficult to study in real time through low-throughput microscopic observation. Therefore, we developed a novel live imaging-based adhesion molecule (LIAM) assay to study gametic adhesion molecule interactions in cultured cells. First, we modified a fusion assay previously established for fusogens introduced into cultured cells, and confirmed that our live imaging technique could visualise cell-to-cell fusion in the modified fusion assay. Next, instead of fusogen, we introduced adhesion molecules including a mammalian gametic adhesion molecule pair, IZUMO and JUNO, and detected their temporal accumulation at the contact interfaces of adjacent cells. Accumulated IZUMO or JUNO was translocated to the opposite cells; the mutation in amino acids required for their interaction impaired accumulation and translocation. By using the novel LIAM assay, we investigated the species specificity of IZUMO and JUNO of mouse, human, hamster, and pig in all combinations. IZUMO and JUNO accumulation and translocation were observed in conspecific, and some interspecific, combinations, suggesting potentially interchangeable combinations of IZUMO and JUNO from different species.
Whole-Genome Sequencing of a Year-Round Fruiting Jackfruit Variety Reveals Very High Single Nucleotide Polymorphisms in Inter-Genic Regions
Authors: Islam Tofazzal , Afroz Nadia , Koh ChuShin , Haque M. Nazmul , Rahman Md. Jillur , Gupta Dipali Rani , Mahmud Nur Uddin , Nahid Abdullah Al , Islam Rashedul , Bhowmik Pankaj K. , Sharpe Andrew G.
Published: December 2021

Background
Jackfruit (Artocarpus heterophyllus Lam.) is a tropical and sub-tropical fruit tree distributed in Asia, Africa, and South America. It is the national fruit of Bangladesh and produces fruit in the summer season only. However, a year-round jackfruit variety, BARI Kanthal-3 developed by Bangladesh Agricultural Research Institute (BARI) provides fruits from September to June. This study aimed to evaluate the agronomic performance of BARI Kanthal-3 and to generate a draft whole genome sequence to obtain molecular insights of this important unique variety.
Results
Number of fruits, average each fruit weight, fruit yield per plant, edible portion in fruit and ß carotene content of BARI Kanthal-3 (n = 5) were 422/plant/year, 5.60 kg, 236.32 kg/year, 53.5% and 3614 mg/100g, respectively. During de novo assembly, 817.7 Mb of the BARI Kanthal-3 genome was scaffolded. However, in the reference-guided genome assembly, almost 843 Mb of the BARI Kanthal-3 genome was scaffolded. Through BUSCO assessment, 97.2% of the core genes were represented in the assembly with 1.3% and 1.5% either fragmented or missing, respectively. By comparing the single copy orthologues (SCOs) in three closely and one distantly related species of BARI Kanthal-3, 706 SCOs were found to be shared across the genomes of the five species. The phylogenetic analysis of the shared SCOs showed that A. heterophyllus is the closest species to BARI Kantal-3. The estimated genome size of BARI Kanthal-3 was 1.04 giga base pairs (Gbp) with a heterozygosity rate of 1.62%. The estimated GC content was 34.10%. Variant analysis revealed that BARI Kanthal-3 includes 5.7 M (35%) and 10.4 M (65%) simple and heterozygous single nucleotide polymorphisms (SNPs), and about 90% of all these polymorphisms are located in inter-genic regions.
Conclusion
The whole-genome sequence of A. heterophyllus cv. BARI Kanthal-3 reveals extremely high single nucleotide polymorphisms in inter-genic regions. The findings of this study will help better understanding the evolution, domestication, phylogenetic relationships, year-round fruiting and the markers development for molecular breeding of this highly nutritious fruit crop.
Sequential Four-Component Synthesis And Antitumor Activity Screening of Spiro[chromene-indolo[2,1-b]quinazoline] And Spiro[indolo[2,1-b]Quinazoline-pyrano[3,2-c]chromene]
Authors: Sadeghian Zahra , Bayat Mohammad , Safari Fatemeh
Published: December 2021

Chemotherapy is one of the most common types of treatment among cancer patients and by using potent chemicals and agents, tumor promotion was inhibited. Despite the usage of many chemical agents in cancer therapy, cancer is still incurable. It seems that the synthesis of new compounds with high efficiency on cancer cells and low side effects on normal cells will remain a critical challenge among researchers in this area. In the present work, a fast and straightforward process for the transformations involving tryptanthrins, malononitrile, some types of CH-acids such as 1,3-cyclohexanedione, dimedone, and 4-hydroxycumarin resulting in preparing spiro[chromene-indolo[2,1-b]quinazoline] and spiro[indolo[2,1-b]quinazoline-pyrano[3,2-c]chromene] derivatives through sequential Knoevenagel/Michael/intramolecular cyclization sequences was reported. at room temperature. This protocol benefits some notable advantages including short reaction time, mild reaction condition, and simple purification, which make it interesting. Furthermore, it was carried out at room temperature, so it is according to green chemistry procedures. Also, antitumor screening of our new synthetic compounds (4a-i) was evaluated on pancreatic cancer cells (Panc1), breast cancer cells (MDA-MB-231), prostate cancer cells (PC3), and normal human adult dermal fibroblast cells (HDF) by using MTT assay using etoposide as a positive control. We found that 50% growth inhibitory concentration (IC50) values of our synthetic compounds were not lower than etoposide against three cancer cell lines.
Using the Asymmetric Gaussian Method to Interpret the Electronic Spectroscopy of the Olivine Family
Authors: Brown Adrian
Published: December 2021

This paper discusses the mathematical aspects of band fitting and introduces the Asymmetric Gaussian curve and its tangent space for the first time. First, we derive an equation for an Asymmetric Gaussian shape. We then derive a rule for the resolution of two Gaussian shaped bands. We then use the Asymmetrical Gaussian equation to derive a Master Equation to fit two overlapping bands. We identify regions of the fitting space where the Asymmetric Gaussian fit is optimal, sub optimal and not optimal. We then demonstrate the use of the Asymmetric Gaussian curve to fit four overlapping Gaussian bands, and show how this is relevant to the olivine family spectral complex at 1 μm. We develop a modified model of the olivine family spectral complex based on previous work by Runciman and Burns. The limitations of the asymmetric band fitting method and a critical assessment of three commonly used numerical minimization methods are also provided.
Complex Function Solution for Deformation and Failure Mechanism of Inclined Coal Seam Roadway
Authors: Xiong Xianyu , Dai Jun , chen Xinnian , Ouyang Yibo
Published: December 2021

The stressed environment of the inclined coal seam roadway is complex and changeable, and the damage degree of surrounding rock increases, threatening the safe mining of coal mines. To improve the effectiveness of stability control of surrounding rock of this kind of roadway, the deformation and failure law of the inclined coal seam roadway is analyzed based on the complex function theory. It optimizes the solution process and accuracy of the mapping function coefficient and deduces the analytical solution of surrounding rock stress and deformation inclined coal seam roadway. The deformation and failure mechanism of surrounding rock in inclined coal seam roadway is revealed theoretically and verified by numerical simulation and physical simulation test. The results show that the stress and deformation of roadway surrounding rock in inclined coal seam show obvious asymmetric distribution characteristics. The stress and deformation of roadway surrounding rock on the right side are greater than on the left side. The two sides of the roadway, the right side of the roof and the roof angle of the right side, are the key positions of roadway stress concentration and deformation. According to the variation law of stress and deformation distribution of roadway surrounding rock, roadway cyclic deformation and failure theory is put forward. The numerical simulation and physical simulation test show that the deformation and failure law of roadway is consistent with the theoretical analysis results, and the cyclic deformation and failure mechanism of roadway in inclined coal seam is verified.
Adaptive Honeycomb Based on Additive Manufacturing: Research on Rapid Generation Algorithm, Manufacturing Process, and Mechanical Characteristics
Authors: Qian Bo , Fan Hongri
Published: December 2021

In order to solve the problems of low efficiency and complex process in the current generation algorithm and process verification of hexagonal honeycomb structures for complex spatial shapes and arbitrarily curved surfaces, this paper proposes an adaptive hexagonal grid calculation method based on the intracellular splitting iteration method for the first time. This method can better adapt to the complex spatial shape and arbitrary curved surface structure in the three-dimensional space, and it can also achieve the purpose of enhancing the mechanical performance while maintaining the lightweight structure. According to the principle of the above algorithm, different structural models including honeycomb cells are calculated and generated. 316L Stainless Steel material and Selective Laser Melting additive manufacturing processes are also used for printing actual samples. The printed samples are mechanically compressed. According to the results of the compression curve, the critical yield force of the honeycomb grid parts with iteration is higher than that of the homogeneous honeycomb grid parts, and the value is basically greater than 30%-40%. Finally, the energy absorption efficiency can be increased by more than 20% according to the compression characteristics of the adaptive iterative honeycomb analyzed.
Microvascular Decompression by Interposition Method for Treatment of Trigeminal Neuralgia Due to Vertebrobasilar Dolichoectasia: A Retrospective Single-Center Study
Authors: Yu Feng , Yin Jia , Lu Pei-gang , Zhao Zhen-yu , Zhang Yong-qiang , Men Xue-zhong
Published: December 2021

Trigeminal neuralgia (TN) due to vertebrobasilar dolichoectasia (VBD) is a rare disease that can be challenging to treat. The objectives of this study are to investigate the characteristics of patients with TN due to VBD and to analyze the efficacy of microvascular decompression (MVD) by the interposition method for treatment of the condition. From 2010 till 2020, the data of 30 patients with TN due to VBD who were treated with MVD by the interposition method were analyzed retrospectively. The characteristics of the patients were compared with those of patients with non-VBD TN (n = 815). Kaplan–Meier survival analysis was performed to determine pain-free survival. The 30 patients (21 males, 9 females; mean age, 63.03 years) accounted for 3.55% of all patients with TN during the study period. In 30 patients, the offending vessel was the basilar artery (BA) in 1 patient, the vertebral artery (VA) in 6 patients, the VA plus the superior cerebellar artery (SCA) in 6 patients, the VA plus the anterior inferior cerebellar artery (AICA) in 12 patients, and the VA+SCA+AICA in 5 patients. Compared to non-VBD TN patients, those with TN due to VBD were significantly more likely to be male, to have TN of the left side, and to have hypertension (all P < 0.001). Mean age at surgery (P = 0.057) and symptom duration (P = 0.308) were comparable between the two groups. All 30 patients had immediate relief of facial pain after MVD and could stop medication. There were no postoperative complications. Over mean follow-up of 76.67 months, 3 patients had recurrence. The mean duration of pain-free survival was 70.77 months. In conclusions, TN due to VBD appears to be more likely in males, in those with hypertension, and to involve the left side. The interposition method performed by experienced and skilled neurosurgeons is a safe and effective treatment for TN due to VBD. Further studies are needed to analyze the associated long-term results and the pain recurrence rate among this special population.
Development of Mechanical Soil Stability In An Initial Homogeneous Loam And Sand Under In-Situ Field Conditions
Authors: Rosskopf Ulla , UTEAU Daniel , PETH Stephan
Published: December 2021

PurposeSoil structure evolving from physical and biological processes is closely related to soil mechanical characteristics and texture. A soil plot experiment in Bad Lauchstädt, Germany, allowed us to study the influence of substrate and genotype on the initial development of mechanical traits, differences between depths, and changes over the course of two years. MethodsPlots were homogeneously filled with a loam and a sand and planted with two maize (Zea mays L.) genotypes (wild type (WT) and rth3 mutant) with contrasting root hair attributes. Undisturbed soil cores were taken in 2019 and 2020 at 14 and 34 cm depth. Confined uniaxial compression tests were performed to determine pre-compression stress (σpc), compressibility (Cc, Cs) and elasticity index (EI). Mechanical energy was calculated based on penetration resistance tests with a penetrometer needle resembling root geometries. Resultsσpc, Cc and Cs were significantly higher in loam as compared to sand, whereas the factor genotype proved to be negligible. Over time, σpc increased and Cc decreased in loam from 2019 to 2020 and Cs declined in both substrates. Higher mechanical energies were observed in loam and partially in WT. Required energy was higher at 14 cm than at 34 cm depth and decreased from 2019 to 2020 in sand. Airdry sand samples required four times as much energy than those at -50 kPa.ConclusionFor the development of the mechanical traits examined texture proved to be the dominating factor and changes in soil stability could be observed within a short period of time.
A Tribometric Device for the Rolling Contact of Soft Elastomers
Authors: Hoyer Brodie , Long Rong , Rentschler Mark E.
Published: December 2021

Rolling contact experimentation is a viable and instructive method for exploring the adhesive contact between surfaces. When applied to soft elastomeric or engineered surfaces, the results of such experiments can provide insights relevant to medical robotics, soft gripping applications, and reversible dry adhesives for bandages or wearable devices. We have designed and built a tribometric device to capture normal and tangential forces between a rolling indenter and substrate correlated with contact area imaging. The device was validated using an experimental setup involving a rigid, nominally smooth acrylic cylinder rolling against a flat polydimethylsiloxame (PDMS) substrate, the results of which matched favorably with accepted contact mechanics theories. The second test involved an indenter with a rigid core and thin (3 mm) smooth shell of a highly deformable, viscoelastic polyvinyl chloride (PVC) rolling on the same PDMS substrate. This test deviated significantly from analytical predictions, highlighting the effects of finite-thickness effects, viscoelasticity, and interfacial slip. This device will facilitate experimental investigations of the rolling contact mechanics between textured surfaces and soft tissue-like materials, which is an important fundamental problem in medical robotics.
County-level Analysis of Perinatal Health Indicators Within a Single Health System Catchment
Authors: Lemas Dominick J. , Layton Claire , Ballard Hailey , Xu Ke , Smulian John C. , Gurka Matthew , Loop Matthew Shane , Smith Erica L. , Reeder Callie F. , Louis-Jacques Adetola , Hsiao Chu J , Cacho Nicole , Hall Jaclyn
Published: December 2021

Background: Adverse perinatal health outcomes are disproportionally impacted in rural communities. Social determinants of health (SDoH) defined by nonclinical social, behavioral, and economic factors may impact up to 90% of health outcomes in rural communities. Objective: To evaluate county-level perinatal patterns in health outcomes, health behaviors, socioeconomic vulnerability, and healthcare providers across rural and non-rural Florida counties within a single health system catchment. Methods: Socioeconomic vulnerability metrics, digital connectivity, licensed provider metrics, and behavioral data and were obtained from Floridahealthcharts.com and the County Health Rankings. County-level birth and perinatal health outcome data were obtained from the Florida Department of Health. The University of Florida Health Perinatal Catchment Area (UFHPCA) was defined as all Florida counties where ≥5% of all infants were delivered at Shands Hospital in Alachua county between June 2011 and April 2017. County-level rurality was determined by Florida Statutes 288.0656 rurality designations. Results: The UFHPCA included three non-rural and ten rural counties that represented more than 64,000 deliveries over a 5-year 9-month period. We found that nearly 1 in 3 infants resided in a rural county (n=20,899), and 7 out of 13 counties did not have a licensed obstetrician gynecologist. Nine counties reported maternal death rates that were between 1 and 4-fold higher than the statewide rate, and rural counties generally reported neonatal mortality and preterm birth rates that were higher than the statewide averages. We found maternal smoking rates (range 6.8% – 24.8%) were above the statewide rate (6.2%) for all counties in the catchment. Except for Alachua county, breastfeeding initiation rates (range 54.9% - 81.4%) and access to household computing devices (range 72.8% - 86.4%) were below the statewide rate (82.9% and 87.9%, respectively). Finally, we found that childhood poverty rates (range 16.3% - 36.9%) in our catchment was above the statewide rate (18.5%), except for Suwanee and Columbia counties.Conclusions: The health burden of the UFHPCA is characterized by both rural and non-rural counties with increased maternal and neonatal death and preterm birth, as well as adverse health behaviors that include smoking during pregnancy and lower levels of breastfeeding.
TaxaTarget: Fast, Sensitive, and Precise Classification of Microeukaryotes in Metagenomic Data
Authors: Commichaux Seth , Javkar Kiran , Muralidharan Harihara Subrahmaniam , Ramachandran Padmini , Ottesen Andrea , Rand Hugh , Pop Mihai
Published: December 2021

BackgroundMicrobial eukaryotes are nearly ubiquitous in microbiomes on Earth and contribute to many integral ecological functions. Metagenomics is a proven tool for studying the microbial diversity, functions, and ecology of microbiomes, but has been underutilized for microeukaryotes due to the computational challenges they present. For taxonomic classification, the use of a eukaryotic marker gene database can improve the computational efficiency, precision and sensitivity. However, state-of-the-art tools which use marker gene databases implement universal thresholds for classification rather than dynamically learning the thresholds from the database structure, impacting the accuracy of the classification process.ResultsHere we introduce taxaTarget, a method for the taxonomic classification of microeukaryotes in metagenomic data. Using a database of eukaryotic marker genes and a supervised learning approach for training, we learned the discriminatory power and classification thresholds for each 20 amino acid region of each marker gene in our database. This approach provided improved sensitivity and precision compared to other state-of-the-art approaches, with rapid runtimes and low memory usage. Additionally, taxaTarget was better able to detect the presence of multiple closely related species as well as species with no representative sequences in the database. One of the greatest challenges faced during the development of taxaTarget was the general sparsity of available sequences for microeukaryotes. Several algorithms were implemented, including threshold padding, which effectively handled the missing training data and reduced classification errors. Using taxaTarget on metagenomes from human fecal microbiomes, a broader range of genera were detected, including multiple parasites that the other tested tools missed.ConclusionData-driven methods for learning classification thresholds from the structure of an input database can provide granular information about the discriminatory power of the sequences and improve the sensitivity and precision of classification. These methods will help facilitate a more comprehensive analysis of metagenomic data and expand our knowledge about the diverse eukaryotes in microbial communities.
The Effects of Microglia on Tauopathy Progression Can Be Quantified Using Nexopathy in Silico (Nexis) Models
Authors: Anand Chaitali , Maia Pedro , Torok Justin , Mezias Christopher , Raj Ashish
Published: December 2021

The prion-like transsynaptic propagation of misfolded tau along the brain’s connectome has previously been modeled using connectome-based network diffusion models. In addition to the connectome, interactions between the general neurological ‘milieu’ in the neurodegenerative brain and proteinopathic species can also contribute to pathology propagation. Such a molecular nexopathy framework posits that the distinct characteristics of neurodegenerative disorders stem from interactions between the network and surrounding molecular players. However, the effects of these modulators remain unquantified. Here, we present Nexopathy in silico (‘Nexis’), a quantitative model of tau progression augmenting earlier models by including parameters of pathology propagation defined by the molecular modulators of connectome-based spread. Our Nexis:microglia model provides the first quantitative characterization of this effect on the whole brain by expanding previous models of neuropathology progression by incorporating microglial influence. We show that Trem2, but not microglial homeostasis genes, significantly improved the model’s predictive power. Trem2 appears to reduce tau accumulation rate while increasing its interregional spread from the hippocampal seed area, causing higher tau burden in the striatum, pallidum, and contralateral hippocampus. Nexis thus provides an improved understanding and quantification of microglial contribution to tau propagation and can be flexibly modified to include other modulators of progressive neurodegeneration.
Sleep Quality and Associated Factors Among Type 2 Dm Patients and Non-Dm Individuals in Bahir Dar Governmental Hospitals: Comparative Cross-Sectional Study
Authors: Zewdu Dagmawit , Gedamu Haileyesus , Beyene Yeshiwork , Tadesse Mekdes , Tamirat Mahlet , Muluken Silenat
Published: December 2021

Background: Multiple factors may contribute to sleep disruption in type 2 diabetic individuals. Sleep disruption in type 2 diabetic individuals is frequently associated with long-term damage, dysfunction, and failure of different organs. Nevertheless, literature in this regard is scanty in Ethiopia. Therefore, this study aimed to assess and compare the prevalence of poor sleep quality and associated factors among type 2 diabetic and non-diabetic individuals in Bahir Dar governmental hospitals.Methods: Comparative cross-sectional study was employed among 292 individuals with type 2 diabetes and 291 non-diabetic individuals in Bahir Dar governmental hospitals from March 01- to April-01. A two-stage cluster sampling method was employed to select participants. Pittsburgh sleep quality index was used for assessing sleep quality. For analysis, descriptive and summary statistics were used to determine the prevalence and percentage of variables. Chi-square test was also used for comparison. Binary logistic regression analysis was employed to determine the associated factors of poor sleep quality. Result: The prevalence of poor sleep was 50.7% (95% CI; 44.9-56.2) and 31.8% (95% CI 26.5-37.5) among individuals with type 2 diabetes and non-diabetic individuals respectively. Among the overall participants being type 2 diabetic patient was also found significantly associated with poor sleep quality as compared to non-diabetic individuals (AOR=1.89; 95% CI; 1.19-2.87). Comorbidity, duration of DM >10 years, Poor glycaemic control, having depression, low physical activity, and poor social support were factors significantly associated with poor sleep quality among individuals with type 2 diabetes. Among non-diabetic individual’s low physical activity, poor social support, having depression, and age group (>50 years) were factors significantly associated with poor sleep quality. Conclusion: In this study, the prevalence of poor sleep among individuals with type 2 diabetes was higher than non-diabetes individuals.
Self-Compassion And Imagery Rescripting: A Brief Online Intervention To Reduce Body Dissatisfaction in Women
Authors: Butler Ashley , Talbot Daniel , Smith Evelyn
Published: December 2021

Background: Due to the prevalence of body dissatisfaction, and given that it is a significant risk factor for eating disorders and obesity, understanding the mechanisms of change, and finding new ways on how to reduce body dissatisfaction are important avenues of research. The current study aimed to investigate whether a brief online intervention of imagery rescripting is more effective in reducing state body dissatisfaction in women than the widely used intervention of self-compassion, or a control condition. Methods: One-hundred and twenty-three Australian women with high levels of body dissatisfaction were allocated to one of the three intervention conditions: imagery rescripting, self-compassion, or control. Participants completed a screening questionnaire, a visual analogue scale (VAS) on mood, and a physical appearance state and trait anxiety scale (PASTAS) on state body dissatisfaction before and after a body dissatisfaction inducing mirror task, and again after the intervention. Results: Results showcased a significant decrease in state body dissatisfaction from post-induction to post-intervention for imagery rescripting compared to the control. Conclusion: Imagery rescripting was identified as an effective brief intervention for reducing state body dissatisfaction in women and inspiring body acceptance compared to the control condition. Future studies should investigate the impact of multiple sessions of imagery rescripting for women with high levels of body dissatisfaction.
Human CSF Movement Influenced by Vascular Low Frequency Oscillations and Respiration
Authors: Nair Vidhya Vijayakrishnan , Kish Brianna R , Inglis Ben , Yang Ho-Ching (Shawn) , Wu Yu-Chien , Zhou Xiaopeng , Schwichtenberg Amy J. , Tong Yunjie
Published: December 2021

Background: Cerebrospinal fluid movement (CSF) through the pathways within the central nervous system is of high significance for maintaining normal brain health and function. Low frequency hemodynamics and respiration have both been shown to independently drive CSF in humans. Here, we hypothesize that CSF movement may be driven simultaneously (and in synchrony) by both mechanisms and we study their independent and interactive effects on CSF movement using novel neck fMRI scans.Methods: Caudad CSF movement at the fourth ventricle and hemodynamics of the major neck blood vessels (internal carotid arteries and internal jugular veins) were captured from 11 young healthy volunteers using novel neck fMRI scans with simultaneous measurement of respiration. Two distinct models of CSF movement (1. Low-frequency hemodynamics and 2. Respiration) were independently investigated in corresponding frequency ranges. Possible interactions between these mechanisms were also studied using cross-frequency coupling.Results: The results from this study validated that the caudad CSF movement may be driven by both low frequency hemodynamics (0.01 Hz – 0.1 Hz) and respiration (0.2 Hz - 0.4 Hz), through different mechanisms. We show that the dynamics of brain fluids can be assessed from the neck, by studying the interrelationships between major neck blood vessels and the CSF movement at the fourth ventricle. We also demonstrate that there exists a cross-frequency interaction between two separable mechanisms.Conclusions: The human CSF system is capable of responding to multiple interacting physiological forces at the same time. This information may help inform the pathological mechanisms behind CSF movement-related disorders and facilitate new approaches to therapeutic interventions.
The Research on Stability of Surrounding Rock in Gob-side Entry Driving in Deep and Thick Seam
Authors: SHI jianjun , Jicheng Feng , Rui Peng , Quanjie Zhu
Published: December 2021

The gob-side entry driving is driving in low pressure area, which bears less support pressure and is easy to maintain, so it is widely used. Taking the gob-side entry driving in thick coal seam of Dongtan Coal Mine as an example, the reasonable size of pillar and the section of roadway are numerically simulated by combining numerical with measurement, and the roadway support is designed. According to the distribution of lateral stress in working face, eight pillars of different sizes are designed. By simulating and comparing the stress distribution of surrounding rock and the development range and shape of plastic zone in different positions, the pillar size of gob-side entry driving is optimized to be 4.5m. According to the results of optimization of roadway section, the section of straight wall semi-circular arch roadway is adopted. According to the analysis, the roadway is supported by bolt + steel mesh + anchor cable. By observing the stability of roadway, it provides experience for the stability study of roadway the gob-side entry driving with small pillar in thick seam.
A Study of Reproducibility of Papanicolaou Society of Cytopathology System of Reporting Respiratory Cytology-A Single Institutional Experience on Image Guided Aspiration Cytology
Authors: Patel Surbhi , Adiga Deepa , Rai Sharada
Published: December 2021

Background: Cytologic examination of specimens obtained from the respiratory tract is a primary and frequently the initial diagnostic technique performed in patients with pulmonary abnormalities. Fine needle aspiration (FNA) is extensively used for diagnosis of pulmonary lesions. The Papanicolaou Society of Cytopathology (PSC) issued a new classification for respiratory cytology and criteria, risk of malignancy, post-cytologic diagnosis management and follow-up. Methods: Respiratory FNA specimens obtained between January 2015 to March 2021 were reviewed and reclassified according to PSC guidelines. Cytologic category as per PSC system was assigned after reviewing by two pathologists. Risk of malignancy for each category was calculated for cases where biopsy was available for correlation. Results: Three eighty-four samples were classified as non-diagnostic (30.5%), negative for malignancy (13.3%), atypical (0.5%), neoplastic (benign/ low malignant potential) (0%), suspicious for malignancy (5.7%%) and malignancy (50%). Risk of malignancy for malignant category (Category V and Category VI) was 64.1% and for non-malignant (Category I to Category IV) cases was 35.9%. Overall, there was lesser incidence of atypical category and more specific diagnoses were possible on cytology in non-malignant category. Sensitivity and specificity for malignant cases was 99.33% and 100% respectively. Conclusion: Our study substantiates that use of PSC guidelines improves the overall reporting of respiratory cytology due to the use of standardized terminology.