bluuebunny/crossref_metadata_2025 huggingface dataset:
Lysinuric protein intolerance presenting as pancytopenia and splenomegaly mimicking acute leukaemia: a case report
Authors: Lokuhewage Chanika , Pathiraja Hashan , Madawala Piyumi , Bandara Sudarshana , Mettananda Sachith
Published: August 2023

Background

Lysinuric protein intolerance is a rare inherited metabolic disease due to autosomal recessive mutations of the SLC7A7 gene. The affected patients commonly present with protein-rich food intolerance, failure to thrive, hepatosplenomegaly, muscle hypotonia and lung involvement due to impaired intestinal absorption and excessive urinary excretion of dibasic amino acids. Presentation with splenomegaly and cytopenia without other features has not been reported. Here we report a Sri Lankan girl with lysinuric protein intolerance presenting with pancytopenia and splenomegaly mimicking acute leukaemia.

Case presentation

Two years and six months old Sri Lankan girl presented with persistent pancytopenia following a viral illness. She was asymptomatic without vomiting, diarrhoea, abdominal pain or irritability. Physical examination revealed pallor and isolated firm splenomegaly of 2 cm. Growth parameters and other system examinations were normal. Full blood count revealed anaemia, leukopenia and thrombocytopenia. The blood picture showed a mixture of hypochromic microcytic and normochromic normocytic red cells with occasional pencil cells and macrocytes. Bone marrow examination was normal except for occasional megaloblasts; however, serum vitamin B12 and red blood cell folate were normal. The metabolic screen showed a high anion gap compensated metabolic acidosis, high lactate and ketosis. Genetic mutation analysis using whole exome sequencing revealed compound heterozygous variants of the SLC7A7 gene, confirming the diagnosis of lysinuric protein intolerance.

Conclusion

We report a child with lysinuric protein intolerance presenting with pancytopenia and splenomegaly without other disease features. This case report adds to the heterogenic presentations of lysinuric protein intolerance, which is considered a multifaceted disease.
Functional connectivity analysis on electroencephalography signals reveals potential biomarkers for treatment response in major depression
Authors: Huang Shiau-Shian , Yu Yu-Hsiang , Chen His-Han , Hung Chia-Chun , Wang Yao-Ting , Chang Chieh Hsin , Peng Syu-Jyun , Kuo Po-Hsiu
Published: August 2023

Background

The treatment efficacy varies across individual patients with major depressive disorder (MDD). It lacks robust electroencephalography (EEG) markers for an antidepressant-responsive phenotype.

Method

This is an observational study enrolling 28 patients with MDD and 33 healthy controls (mean age of 40.7 years, and 71.4% were women). Patients underwent EEG exams at baseline (week0) and week1, while controls’ EEG recordings were acquired only at week0. A resting eye-closing EEG segment was analyzed for functional connectivity (FC). Four parameters were used in FC analysis: (1) node strength (NS), (2) global efficiency (GE), (3) clustering coefficient (CC), and (4) betweenness centrality (BC).

Results

We found that controls had higher values in delta wave in the indices of NS, GE, BC, and CC than MDD patients at baseline. After treatment with antidepressants, patients’ FC indices improved significantly, including GE, mean CC, and mean NS in the delta wave. The FC in the alpha and beta bands of the responders were higher than those of the non-responders.

Conclusions

The FC of the MDD patients at baseline without treatment was worse than that of controls. After treatment, the FC improved and was close to the values of controls. Responders showed better FC in the high-frequency bands than non-responders, and this feature exists in both pre-treatment and post-treatment EEG.
Does repeated autoclave sterilization cause changes in the color and fragility of fiberglass reinforced resin crowns?
Authors: Delikan Ebru , Çaliskan Seçil , Çalışkan Ahmet , Özdemir Canan
Published: August 2023

Background

Since the effects of sterilization on the Fiberglass Reinforced Resin Crowns (FRRCs) is not well-known the aim of current study was to evaluate the effects of autoclave sterilization on the fracture resistance, color stability, and surface composition of FRRCs.

Methods

A total of 48 crowns were used. The crowns were divided into three groups according to the sterilization number: no sterilization (Control Group), one sterilization (Group 1), and four sterilizations (Group 2). The microstructure of the three crowns from each group was evaluated using scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX) spectroscopy. Thirteen FRRCs from each group were first used for color stability testing and then for the fracture resistance analysis. One-way analysis of variance (ANOVA), one-way repeated measures ANOVA, and paired t-tests were used in the statistical analysis.

Results

EDX results revealed that the weight% of surface silicon atoms in group 2 was significantly higher. Some crack lines could be observed on the SEM images. Statistically significant differences were found in color stability following the first and fourth sterilization cycles (p < .01). The increase in the sterilization cycle did not statistically decrease the fracture resistance of the FRRCs (p = .055); however, overall, a decreasing trend was observed in fracture resistance as the sterilization cycle increased.

Conclusions

Autoclave sterilization caused some changes in the surface elemental composition and surface morphology of FRRCs. Avoiding unnecessary FRRC trials is important to reduce the number of sterilizations.
The effectiveness of problem-based learning compared with lecture-based learning in surgical education: a systematic review and meta-analysis
Authors: Zheng Qi-Ming , Li Yuan-Yuan , Yin Qing , Zhang Na , Wang Ye-Peng , Li Guo-Xiang , Sun Zhi-Gang
Published: August 2023

Background

This meta-analysis was conducted to systematically evaluate the impact of problem-based learning (PBL) and lecture-based learning (LBL) teaching models on students’ learning in surgical education.

Methods

We systematically searched the publications related to the application of PBL and LBL in surgical courses in PubMed, Embase, Web of Science and Cochrane Library databases, the last retrieval time is September 20, 2022. After screening the literature according to the inclusion and exclusion criteria, extracting data and evaluating the methodological treatment of the included studies, Stata 17.0 software was used to perform meta-analysis.

Results

Nine studies were included totally. The results showed that compared with LBL, PBL was superior in clinical competence (SMD = 0.81, 95% CI: 0.12 ~ 1.49, P = 0.020) and student satisfaction (SMD = 2.13, 95% CI: 1.11 ~ 3.15, P < 0.0001) with significant differences. But the comprehensive scores (SMD = 0.26, 95% CI: -0.37 ~ 0.89, P = 0.421) and theoretical knowledge (SMD=−0.19, 95% CI: −0.71 ~ 0.33, P = 0.482) to PBL and LBL had no significant difference.

Conclusion

This study showed that the PBL teaching model is more effective than the LBL teaching model in surgical education on the aspects of enhancing clinical competence and student satisfaction. However, further well-designed studies are needed to confirm our findings.
M7G methylated core genes (METTL1 and WDR4) and associated RNA risk signatures are associated with prognosis and immune escape in HCC
Authors: Li Rui , Liu Xincheng , Deng Kaiyuan , Wang Xin
Published: August 2023

N7 methylguanosine (m7G) has a crucial role the development of hepatocellular carcinoma (HCC). This study aimed to investigate the impact of the m7G methylation core genes (METTL1 and WDR4) and associated RNA risk signatures on HCC. we found m7G methylation core genes (METTL1 and WDR4) were upregulated in four HCC cell lines, and downregulation of METTL1 and WDR4 attenuated HCC cell proliferation, migration, and invasion. Moreover, METTL1 and WDR4 are upregulated in HCC tissues, and that there is a significant positive correlation between them. METTL1 and WDR4 were identified as independent prognostic markers for HCC by employing overall survival (OS), disease-specific survival (DSS), Progression Free Interval survival (PFI), and univariate/multivariate Cox analyses. We identified 1479 coding RNAs (mRNAs) and 232 long non-coding RNAs (lncRNAs) associated with METTL1 / WDR4 by using weighted coexpression network analysis (WGCNA) and co-clustering analysis. The least absolute shrinkage and selection operator (lasso) were used to constructing mRNA and lncRNA risk signatures associated with the METTL1 / WDR4. These risk were independent poor prognostic factors in HCC. Furthermore, we found that METTL1 / WDR4 expression and mRNA / lncRNA risk scores were closely associated with TP53 mutations. Clinicopathological features correlation results showed that METTL1 / WDR4 expression and mRNA / lncRNA risk score were associated with the stage and invasion depth (T) of HCC. To predict the overall survival of HCC individuals, we constructed a nomogram with METTL1/WDR4 expression, mRNA/lncRNA risk score, and clinicopathological features. In addition, we combined single-cell sequencing datasets and immune escape-related checkpoints to construct an immune escape-related protein–protein interaction(PPI) network. In conclusion, M7G methylated core genes (METTL1 and WDR4) and associated RNA risk signatures are associated with prognosis and immune escape in HCC.
Exosomal hsa_circ_000200 as a potential biomarker and metastasis enhancer of gastric cancer via miR-4659a/b-3p/HBEGF axis
Authors: Huang Xiao-juan , Wang Yan , Wang Hui-ting , Liang Zhao-feng , Ji Cheng , Li Xiao-xi , Zhang Lei-lei , Ji Run-bi , Xu Wen-rong , Jin Jian-hua , Qian Hui
Published: August 2023

Background

Exosome, a component of liquid biopsy, loaded protein, DNA, RNA and lipid gradually emerges as biomarker in tumors. However, exosomal circRNAs as biomarker and function mechanism in gastric cancer (GC) are not well understood.

Methods

Differentially expressed circRNAs in GC and healthy people were screened by database. The identification of hsa_circ_000200 was verified by RNase R and sequencing, and the expression of hsa_circ_000200 was evaluated using qRT-PCR. The biological function of hsa_circ_000200 in GC was verified in vitro. Western blot, RIP, RNA fluorescence in situ hybridization, and double luciferase assay were utilized to explore the potential mechanism of hsa_circ_000200.

Results

Hsa_circ_000200 up-regulated in GC tissue, serum and serum exosomes. Hsa_circ_000200 in serum exosomes showed better diagnostic ability than that of tissues and serum. Combined with clinicopathological parameters, its level was related to invasion depth, TNM staging, and distal metastasis. Functionally, knockdown of hsa_circ_000200 inhibited GC cells proliferation, migration and invasion in vitro, while its overexpression played the opposite role. Importantly, exosomes with up-regulated hsa_circ_000200 promoted the proliferation and migration of co-cultured GC cells. Mechanistically, hsa_circ_000200 acted as a “ceRNA” for miR-4659a/b-3p to increase HBEGF and TGF-β/Smad expression, then promoted the development of GC.

Conclusions

Our findings suggest that hsa_circ_000200 promotes the progression of GC through hsa_circ_000200/miR-4659a/b-3p/HBEGF axis and affecting the expression of TGF-β/Smad. Serum exosomal hsa_circ_000200 may serve as a potential biomarker for GC.
Hyaluronic acid-graphene oxide quantum dots nanoconjugate as dual purpose drug delivery and therapeutic agent in meta-inflammation
Authors: Sarkar Kunal , Bank Sarbashri , Chatterjee Arindam , Dutta Koushik , Das Anwesha , Chakraborty Santanu , Paul Nirvika , Sarkar Jit , De Sriparna , Ghosh Sudakshina , Acharyya Krishnendu , Chattopadhyay Dipankar , Das Madhusudan
Published: August 2023

Type 2 diabetes mellitus (T2DM) predominantly considered a metabolic disease is now being considered an inflammatory disease as well due to the involvement of meta-inflammation. Obesity-induced adipose tissue inflammation (ATI) is one of the earliest phenomena in the case of meta-inflammation, leading to the advent of insulin resistance (IR) and T2DM. The key events of ATI are orchestrated by macrophages, which aggravate the inflammatory state in the tissue upon activation, ultimately leading to systemic chronic low-grade inflammation and Non-Alcoholic Steatohepatitis (NASH) through the involvement of proinflammatory cytokines. The CD44 receptor on macrophages is overexpressed in ATI, NASH, and IR. Therefore, we developed a CD44 targeted Hyaluronic Acid functionalized Graphene Oxide Quantum Dots (GOQD-HA) nanocomposite for tissue-specific delivery of metformin. Metformin-loaded GOQD-HA (GOQD-HA-Met) successfully downregulated the expression of proinflammatory cytokines and restored antioxidant status at lower doses than free metformin in both palmitic acid-induced RAW264.7 cells and diet induced obese mice. Our study revealed that the GOQD-HA nanocarrier enhanced the efficacy of Metformin primarily by acting as a therapeutic agent apart from being a drug delivery platform. The therapeutic properties of GOQD-HA stem from both HA and GOQD having anti-inflammatory and antioxidant properties respectively. This study unravels the function of GOQD-HA as a targeted drug delivery option for metformin in meta-inflammation where the nanocarrier itself acts as a therapeutic agent.

Graphical Abstract
Terminally exhausted CD8+ T cells contribute to age-dependent severity of respiratory virus infection
Authors: Parks Olivia B. , Eddens Taylor , Sojati Jorna , Lan Jie , Zhang Yu , Oury Tim D. , Ramsey Manda , Erickson John J. , Byersdorfer Craig A. , Williams John V.
Published: August 2023

Background

Lower respiratory infections are a leading cause of severe morbidity and mortality among older adults. Despite ubiquitous exposure to common respiratory pathogens throughout life and near universal seropositivity, antibodies fail to effectively protect the elderly. Therefore, we hypothesized that severe respiratory illness in the elderly is due to deficient CD8+ T cell responses.

Results

Here, we establish an aged mouse model of human metapneumovirus infection (HMPV) wherein aged C57BL/6 mice exhibit worsened weight loss, clinical disease, lung pathology and delayed viral clearance compared to young adult mice. Aged mice generate fewer lung-infiltrating HMPV epitope-specific CD8+ T cells. Those that do expand demonstrate higher expression of PD-1 and other inhibitory receptors and are functionally impaired. Transplant of aged T cells into young mice and vice versa, as well as adoptive transfer of young versus aged CD8+ T cells into Rag1−/− recipients, recapitulates the HMPV aged phenotype, suggesting a cell-intrinsic age-associated defect. HMPV-specific aged CD8+ T cells exhibit a terminally exhausted TCF1/7− TOX+ EOMES+ phenotype. We confirmed similar terminal exhaustion of aged CD8+ T cells during influenza viral infection.

Conclusions

This study identifies terminal CD8+ T cell exhaustion as a mechanism of severe disease from respiratory viral infections in the elderly.
Sodium butyrate enhances titanium nail osseointegration in ovariectomized rats by inhibiting the PKCα/NOX4/ROS/NF-κB pathways
Authors: Liu Zhiyi , Yao Xuewei , Jiang Wenkai , Zhou Zhi , Yang Min
Published: August 2023

Background

Elevated levels of oxidative stress as a consequence of estrogen deficiency serve as a key driver of the onset of osteoporosis (OP). In addition to increasing the risk of bone fractures, OP can reduce the bone volume proximal to titanium nails implanted to treat these osteoporotic fractures, thereby contributing to titanium nail loosening. Sodium butyrate (NaB) is a short-chain fatty acid produced by members of the gut microbiota that exhibits robust antioxidant and anti-inflammatory properties.

Methods

OP fracture model rats parameters including bone mineral density (BMD), new bone formation, and the number of bonelets around the implanted nail were analyzed via micro-CT scans, H&E staining, and Masson’s staining. The protective effects of NaB on such osseointegration and the underlying mechanisms were further studied in vitro using MC3T3-E1 cells treated with carbonyl cyanide m-chlorophenylhydrazone (CCCP) to induce oxidative stress. Techniques including Western immunoblotting, electron microscopy, flow cytometry, alkaline phosphatase (ALP) staining, and osteoblast mineralization assays were employed to probe behaviors such as reactive oxygen species production, mineralization activity, ALP activity, protein expression, and the ability of cells to attach to and survive on titanium plates.

Results

NaB treatment was found to enhance ALP activity, mineralization capacity, and Coll-I, BMP2, and OCN expression levels in CCCP-treated MC3T3-E1 cells, while also suppressing PKC and NF-κB expression and enhancing Nrf2 and HO-1 expression in these cells. NaB further suppressed intracellular ROS production and malondialdehyde levels within the cytosol while enhancing superoxide dismutase activity and lowering the apoptotic death rate. In line with these results, in vivo work revealed an increase in BMD in NaB-treated rats that was associated with enhanced bone formation surrounding titanium nails.

Conclusion

These findings indicate that NaB may represent a valuable compound that can be postoperatively administered to aid in treating OP fractures through the enhancement of titanium nail osseointegration.
The effect of dual-task conditions on postural control in adults with low back pain: a systematic review and meta-analysis
Authors: Pourahmadi Mohammadreza , Negahban Hossein , Koes Bart Willem , Fernández‐de‐Las‐Peñas César , Ebrahimi Takamjani Ismail , Bahramian Mehrdad
Published: August 2023

Background

Dual-task conditions, which involve performing two tasks simultaneously, may exacerbate pain and further impair daily functioning in individuals with low back pain (LBP). Understanding the effects of dual-task conditions on postural control in patients with LBP is crucial for the development of effective rehabilitation programs. Our objective was to investigate the impact of dual-task conditions on postural control in individuals with LBP compared to those without LBP.

Methods

We conducted a comprehensive search of Medline via PubMed, Scopus, the Cochrane Central Register of Controlled Trials, Web of Science, and EMBASE databases, with no language restrictions, from inception to January 1, 2023. The primary outcome measures of the study were velocity, area, amplitude, phase plane portrait, and path/sway length of the center of pressure (CoP). Standardized mean difference (SMD) effect sizes were calculated, and the quality of the studies was assessed using the Newcastle–Ottawa Scale (NOS).

Results

From 196 studies, five involving 242 adults (≥ 18 years) met the inclusion criteria. Three studies were rated as high quality, while two were deemed moderate. In the included studies, 140 participants had non-specific LBP, while 102 participants did not report any symptoms, with mean ages of 36.68 (± 14.21) and 36.35 (± 15.39) years, respectively. Three studies had both genders, one exclusively included females, and one did not specify gender. Meta-analyses of primary outcomes revealed no significant differences in postural control between patients with LBP and pain-free controls during both easy and difficult postural tasks and cognitive load for velocity (easy: SMD − 0.09, 95% CI − 0.91 to 0.74; difficult: SMD 0.12, 95% CI − 0.67 to 0.91), area (easy: SMD 0.82, 95% CI − 2.99 to 4.62; difficult: SMD 0.14, 95% CI − 2.62 to 2.89), phase plane (easy: SMD − 0.59, 95% CI − 1.19 to 0.02; difficult: SMD − 0.18, 95% CI − 0.77 to 0.42), path/sway length (easy: SMD − 0.18, 95% CI − 0.77 to 0.42; difficult: SMD − 0.14, 95% CI − 0.84 to 0.55), and amplitude (easy: SMD 0.89, 95% CI − 1.62 to 3.39; difficult: SMD 1.31, 95% CI − 1.48 to 4.10).

Conclusions

The current evidence suggests that there are no significant differences in postural control parameters during dual-task conditions between individuals with non-specific LBP and pain-free subjects. However, due to the limited number of available studies, significant publication bias, and considerable statistical heterogeneity, definitive conclusions cannot be drawn. Therefore, further research comprising high-quality studies with larger sample sizes is necessary to obtain conclusive results.

Trial registration PROSPERO CRD42022359263.
Australian podiatry workforce: findings from the PAIGE cross‐sectional study of Australian podiatrists
Authors: Couch Anna , Haines Terry , O'Sullivan Belinda , Menz Hylton B. , Williams Cylie M.
Published: August 2023

Background

Understanding the dynamics of the podiatry workforce is essential for the sustainability of the profession. This study aimed to describe the podiatry workforce characteristics and identify factors associated with rural practice location.

Methods

We used an exploratory descriptive design from data obtained during cross sectional study: Podiatrists in Australia: Investigating Graduate Employment through four online surveys (2017–2020). Demographic and workplace characteristics including career development were described. Univariate logistic regressions were used to determine associations with rural or metropolitan practice location.

Results

Data were included from 1, 135 podiatrists (21% of n = 5,429). There were 716 (69% of n = 1,042) females, 724 (65% of n = 1,118) worked in the public health service and 574 (51% of 1,129) were salaried employees. There were 706 (87% of n = 816) podiatrists with access to paid annual leave and 592 (72% of n = 816) to paid sick leave. There were 87 (32% of n = 276) podiatrists who reported 51–75% of workload involved Medicare bulk‐billed Chronic Disease Management plans, and 324 (74% of n = 436) not utilising telehealth. The majority of podiatrists (57% of n = 1,048) indicated their average consultation length was 21 ‐30 min, and patients typically waited < 3 days for an appointment (41% of n = 1,043). Univariate logistic regression identified podiatrists working in metropolitan settings have less years working in current location (OR = 0.98, 95% CI = 0.96, 0.99), less working locations (OR = 0.91, 95% CI = 0.86, 0.97), were less likely to have access to paid annual leave (OR = 0.65, 95% CI = 0.43, 0.98), and paid sick leave (OR = 0.65, 95% CI = 0.46, 0.95), shorter waiting periods for appointments (OR = 0.44, 95% CI 0.30, 0.64) and more likely to utilise telehealth within their practice (OR = 2.03, 95% CI 1.19, 3.50) than those in rural locations.

Conclusion

These results provide insight into the profession uncommonly captured in workforce planning data. This included the number of working locations, billing practices and wait lists. This also highlights opportunities to promote rural training pathways, service integration to build attractive podiatry positions that are tailored to meet the needs of rural communities and solutions to make telehealth more accessible to podiatrists.
Sample size estimation in clinical trials using ventilator-free days as the primary outcome: a systematic review
Authors: Renard Triché Laurent , Futier Emmanuel , De Carvalho Manuela , Piñol-Domenech Nathalie , Bodet-Contentin Laëtitia , Jabaudon Matthieu , Pereira Bruno
Published: August 2023

Background

Ventilator-free days (VFDs) are a composite endpoint increasingly used as the primary outcome in critical care trials. However, because of the skewed distribution and competitive risk between components, sample size estimation remains challenging. This systematic review was conducted to systematically assess whether the sample size was congruent, as calculated to evaluate VFDs in trials, with VFDs’ distribution and the impact of alternative methods on sample size estimation.

Methods

A systematic literature search was conducted within the PubMed and Embase databases for randomized clinical trials in adults with VFDs as the primary outcome until December 2021. We focused on peer-reviewed journals with 2021 impact factors greater than five. After reviewing definitions of VFDs, we extracted the sample size and methods used for its estimation. The data were collected by two independent investigators and recorded in a standardized, pilot-tested forms tool. Sample sizes were calculated using alternative statistical approaches, and risks of bias were assessed with the Cochrane risk-of-bias tool.

Results

Of the 26 clinical trials included, 19 (73%) raised “some concerns” when assessing risks of bias. Twenty-four (92%) trials were two-arm superiority trials, and 23 (89%) were conducted at multiple sites. Almost all the trials (96%) were unable to consider the unique distribution of VFDs and death as a competitive risk. Moreover, significant heterogeneity was found in the definitions of VFDs, especially regarding varying start time and type of respiratory support. Methods for sample size estimation were also heterogeneous, and simple models, such as the Mann–Whitney–Wilcoxon rank-sum test, were used in 14 (54%) trials. Finally, the sample sizes calculated varied by a factor of 1.6 to 17.4.

Conclusions

A standardized definition and methodology for VFDs, including the use of a core outcome set, seems to be required. Indeed, this could facilitate the interpretation of findings in clinical trials, as well as their construction, especially the sample size estimation which is a trade-off between cost, ethics, and statistical power.

Systematic review registration PROSPERO ID: CRD42021282304. Registered 15 December 2021 (https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42021282304).
Shared genetic architecture between irritable bowel syndrome and psychiatric disorders reveals molecular pathways of the gut-brain axis
Authors: Tesfaye Markos , Jaholkowski Piotr , Hindley Guy F. L. , Shadrin Alexey A. , Rahman Zillur , Bahrami Shahram , Lin Aihua , Holen Børge , Parker Nadine , Cheng Weiqiu , Rødevand Linn , Frei Oleksandr , Djurovic Srdjan , Dale Anders M. , Smeland Olav B. , O’Connell Kevin S. , Andreassen Ole A.
Published: August 2023

Background

Irritable bowel syndrome (IBS) often co-occurs with psychiatric and gastrointestinal disorders. A recent genome-wide association study (GWAS) identified several genetic risk variants for IBS. However, most of the heritability remains unidentified, and the genetic overlap with psychiatric and somatic disorders is not quantified beyond genome-wide genetic correlations. Here, we characterize the genetic architecture of IBS, further, investigate its genetic overlap with psychiatric and gastrointestinal phenotypes, and identify novel genomic risk loci.

Methods

Using GWAS summary statistics of IBS (53,400 cases and 433,201 controls), and psychiatric and gastrointestinal phenotypes, we performed bivariate casual mixture model analysis to characterize the genetic architecture and genetic overlap between these phenotypes. We leveraged identified genetic overlap to boost the discovery of genomic loci associated with IBS, and to identify specific shared loci associated with both IBS and psychiatric and gastrointestinal phenotypes, using the conditional/conjunctional false discovery rate (condFDR/conjFDR) framework. We used functional mapping and gene annotation (FUMA) for functional analyses.

Results

IBS was highly polygenic with 12k trait-influencing variants. We found extensive polygenic overlap between IBS and psychiatric disorders and to a lesser extent with gastrointestinal diseases. We identified 132 independent IBS-associated loci (condFDR < 0.05) by conditioning on psychiatric disorders (n = 127) and gastrointestinal diseases (n = 24). Using conjFDR, 70 unique loci were shared between IBS and psychiatric disorders. Functional analyses of shared loci revealed enrichment for biological pathways of the nervous and immune systems. Genetic correlations and shared loci between psychiatric disorders and IBS subtypes were different.

Conclusions

We found extensive polygenic overlap of IBS and psychiatric and gastrointestinal phenotypes beyond what was revealed with genetic correlations. Leveraging the overlap, we discovered genetic loci associated with IBS which implicate a wide range of biological pathways beyond the gut-brain axis. Genetic differences may underlie the clinical subtype of IBS. These results increase our understanding of the pathophysiology of IBS which may form the basis for the development of individualized interventions.
Sex differences in song syntax and syllable diversity in testosterone-induced songs of adult male and female canaries
Authors: dos Santos Ednei B. , Ball Gregory F. , Logue David M. , Cornil Charlotte A. , Balthazart Jacques
Published: August 2023

Background

Behavioral sex differences are widespread in the animal world. These differences can be qualitative (i.e., behavior present in one sex but not the other, a true sex dimorphism) or quantitative (behavior is present at a higher rate or quality in one sex compared to the other). Singing in oscine songbirds is associated with both types of differences. In canaries, female rarely sing spontaneously but they can be induced to do so by treatments with steroids. Song in these females is, however, not fully masculinized and exhibits relatively subtle differences in quality as compared with male song. We analyzed here sex differences in syllable content and syllable use between singing male and female canaries.

Methods

Songs were recorded from three groups of castrated male and three groups of photoregressed female canaries that had received Silastic™ implants filled with testosterone (T), with T plus estradiol (E2), or left empty (control). After 6 weeks of hormone treatment, 30 songs were recorded from each of the 47 subjects. Songs were segmented and each syllable was annotated. Various metrics of syllable diversity were extracted and network analysis was employed to characterize syllable sequences.

Results

Male and female songs were characterized by marked sex differences related to syllable use. Compared to females, males had a larger syllable-type repertoire and their songs contained more syllable types. Network analysis of syllable sequences showed that males follow more fixed patterns of syllable transitions than females. Both sexes, however, produced song of the same duration containing the same number of syllables produced at similar rates (numbers per second).

Conclusions

Under the influence of T, canaries of both sexes are able to produce generally similar vocalizations that nevertheless differ in specific ways. The development of song during ontogeny appears to be a very sophisticated process that is presumably based on genetic and endocrine mechanisms but also on specific learning processes. These data highlight the importance of detailed behavioral analyses to identify the many dimensions of a behavior that can differ between males and females.
Analysis of differences in tobacco leaf microbial communities after redrying in Chinese provinces and from abroad
Authors: Zhang Yifan , Xu Qiang , Yang Mengmeng , Yang Yue , Fu Jincun , Miao Chenlin , Wang Guiyao , Hu Liwei , Hu Zongyu
Published: August 2023

Microorganisms play an important role in the tobacco aging process. Before the aging process, raw tobacco leaves must be threshed and redried. In order to explore the differences of microbial community structure of threshed and redried tobacco leaves from different origins at home and abroad, 14 groups of tobacco leaves from 8 different countries were tested by high-throughput DNA sequencing and microbiology analysis. Then, through amplicon sequence variants (ASV) cluster analysis, Venn diagram and species labeling and other microbial diversity analysis, the dominant bacteria and fungi on the surface of threshed and redried tobacco leaves were obtained. The results showed that there were significant differences in the composition of tobacco bacteria and fungi after threshing and redrying from different geographical areas. The relative abundance of Microbacterium and Sphingomonas in domestic tobacco leaves was significantly higher than that of foreign tobacco leaves. The relative abundance of Pseudomonas in foreign tobacco bacterial colonies was significantly higher than that of domestic tobacco leaves. In terms of fungi, the relative abundance of Aspergillus and Alternaria in domestic tobacco leaves was significantly higher than that of foreign tobacco leaves. Septoria, Sampaiozyma, Cladosporium and Phoma account for significantly higher proportions of foreign tobacco leaves. These microorganisms may be indispensable in aging process to form different flavors of tobacco leaves. It provides an important theoretical basis for the further use of microorganisms to promote tobacco leaf aging.
An efficient and scalable vaccine passport verification system based on ciphertext policy attribute-based encryption and blockchain
Authors: Fugkeaw Somchart
Published: August 2023

Implementing a trust and secure immunity or vaccine passport verification system is now crucial for many countries. The system typically aims to enable the secure access control and verification of vaccination records which will be used by trusted parties. However, the issues related to the system scalability in supporting a large number of data access requests, the enforcement of the user consent for data sharing, and the flexibility in delegating the access capability to trusted parties have not been resolved by existing works. In this paper, we propose a Universal Vaccine Passport Verification System (UniVAC) to support a decentralized, scalable, secure, and fine-grained, access control for Covid-19 vaccine passport data sharing and verification. At a core of our scheme, we employ the ciphertext policy attribute-based encryption (CP-ABE) to support secure and fine-grained access control and use the blockchain to record access transactions and provide data indexing. Furthermore, we propose a ciphertext retrieval method based on regional blockchain segmentation and introduce the outsourced CP-ABE decryption as a part of the proxy re-encryption (PRE) process to enable scalable and secure ciphertext delivery of the encrypted vaccine passport under the requestor’s public key. Finally, we conducted the extensive experiments in real cloud environment and the results showed that our proposed scheme is more efficient and scalable than related works.
Liquid chromatography as analytical tool for the study of natural and early synthetic dyes in traditional Saxon textiles
Authors: Petroviciu Irina , Teodorescu Iulia , Vasilca Silvana , Albu Florin , Medvedovici Andrei
Published: August 2023

Confirmed since the twelth century, the Saxon community in Transylvania developed over the years in a rigorous powerful society, with its own lifestyle, social, economic, cultural and artistic standards. Together with research in historic documents, this society is now revealed by material studies of eighteenth– twentieth century objects in the Emil Sigerius collection, preserved in the ASTRA Museum, Sibiu, Romania. Embroideries made in Saxon households as well as representative Saxon coats manufactured in workshops, and dated between 1892 and 1908 (years embroidered on objects), were studied in terms of dye analysis, in order to understand if adoption of new materials goes together with the stylistic improvements. Dye analysis were performed by liquid chromatography with UV–vis and mass spectrometric detection (LC-DAD-MS), nowadays the most appropriate technique for the characterization and identification of colour components in heritage textiles. MS detection, with a triple quadrupole mass analyser, was used to achieve clear identification of each dye in a complex matrix. Construction of suitable spectral libraries through studies on standard dyes and dyed textiles definitely improved the ability to detect natural and early synthetic with greater certainty. Identification of fuchsine (1856), methyl violet (1861), orange II (1876) brilliant green (1879), quinoline yellow (1881) and rhodamine B (1887) in textiles dated about 20 years after the dyes first synthesis prove that transition from natural to synthetic dyes in the Saxon community was very fast, especially for textiles made in specialized workshops. The analytical configurations used prove their competence in textile studies, based on natural and early synthetic dyes research, and promotes the use of advanced analytical techniques in heritage science applications.
The synergistic effect of biochar and poly(2-ethyl-2-oxazoline)/poly(2-hydroxyethylmethacarylate)/chitosan) hydrogels on saline soil properties and carrot productivity
Authors: Abdeen Sayed A. , Hefni Hassan H. H. , Awadallah-F Ahmed , El-Rahman Nasser R. Abd
Published: August 2023

Background

Soil salinity is one of the most important factors limiting crop production. Furthermore, with the increasing population and saline soil worldwide there is no choice but to utilize saline soil to increase the agricultural regions. Therefore, to improve carrot productivity under saline conditions, it is necessary to provide good management such as applying hydrogels and biochar for improving soil properties.

Methodology

Hydrogels (PEtOx-HEMA-CS) were synthesized from poly (2-ethyl-2-oxazoline), 2-hydroxyethyl methacrylate (HEMA as crosslinker) and chitosan (CS) via exposure those to gamma irradiation dose; 30 kGy of dose rate 0.9 kGy/h and obtained three types of hydrogels according to concentration of chitosan used. The PEtOx-HEMA-CS hydrogels were enhanced water holding capacity for agriculture purposes. The chemical structures of obtained hydrogels were characterized by FTIR, XRD and SEM. The swelling (%) and gelation (%) were determined. Biochar (BC) as an active substance was physically mixed with those hydrogels at various ratios (0/100, 0.5/99.5, 1/99 and 100/0 (g/g) biochar/hydrogels). BC, PEtOx-HEMA-CS and the mixture of PEtOx-HEMA-CS-BC were mixed with saline soil at ratio 0.05% and 0.1% w/w of obtained materials/soil. A pot experiment was conducted to mitigate the salinity hazards on carrot productivity using biochar with and without hydrogels. Mean maximum temperature, minimum temperature, precipitation, relative humidity and wind speed from September to December in the studied region are 28.66 ℃, 15.76 ℃, 0.01 mm, 58.81%, 5.94 km/h, respectively.

Findings

The obtained data referred that there is a significant decrease in soil salinity and exchangeable sodium percentage and increase in organic matter, cation exchange capacity, field capacity, permanent wilting point and available water especially at (PEtOx-HEMA-CS5)0.1-BC1. The highest increment percentage of nitrogen, phosphorous and potassium were 36.36%, 70% and 72%, respectively. In addition, the relative increase of carrot productivity was 49.63% at the highest rates of biochar and hydrogels. However, the highest value of water use efficiency was observed at the mixture of biochar and hydrogels at (PEtOx-HEMA-CS5)0.1-BC1.

Conclusions

Finally, applying biochar combined with (PEtOx-HEMA-CS5) could be recommended as a good approach to improve carrot productivity and water use efficiency under saline soil conditions.

Graphical Abstract
On the use of prior distributions in bayesian inference applied to Ecology: an ecological example using binomial proportions in exotic plants, Central Chile
Authors: Bustamante Ramiro O. , Iturriaga Andrés , Flores-Alvarado Sandra , García Rafael A. , Goncalves Estefany
Published: August 2023

Background

The use of Bayesian inference (BI) is a common methodology for data analysis in Ecology and Evolution. This statistical approach is particularly useful in cases which information is scarce, because allows formalizing sources of information, other than sampling data (priors), obtained from technical reports, expert opinions and beliefs. Recent reviews detected that most ecological studies use non-informativepriorswithout any justification, ignoring other sources of independent information available to construct informativepriors. In this study, we examined how the selection of informative or non-informative priors, affects hypothesis testing. We compared the proportion of occupied sites (occupancy) in four exotic plant species living in two contrasting environments in Central Chile. Given that occupancy is related to binomial proportions, we developed a statistical procedure based on beta distribution, to compare occupancies using Bayes factor.

Results

Bayes factor obtained from different non-informative priors led to similar inferences relative to H0. The use of informative prior drastically changed our decisions about H0in three of four plant species.

Conclusions

The selection of priors is critical because they determine hypothesis testing. The use of independent information will improve our inferences, which is precisely the strength of BI. We hypothesize that the reluctance to use informative priors in ecological studies reflects extreme positivism and the use of non-informative priors is a strategy to avoid subjectivity; by doing that, ecologists depart from the philosophy of BI which accepts that the subjective knowledge is a valid, and sometimes the only alternative, to know the world.
Large bowel perforation due to cytomegalovirus colitis in a post-cardiac surgical patient on chronic hemodialysis
Authors: Fukunaga Naoto , Wakami Tatsuto , Shimoji Akio , Maeda Toshi , Mori Otohime , Yoshizawa Kosuke , Okada Tatsuji , Tamura Nobushige
Published: August 2023

Background

Cytomegalovirus (CMV) colitis with colonic perforation is an exceedingly rare but life-threatening condition. The most comorbid diagnosis in patients with perforated CMV colitis is human immunodeficiency virus in the setting of advanced immunosuppression associated with CD4 < 50 cells/μL.

Case presentation

A 75-year-old female with a ≥ 30-year history of hemodialysis presented with progressive dyspnea on exertion. Transthoracic echocardiography showed moderate mitral stenosis and severe aortic stenosis. Although she was a high-risk candidate with a risk for mortality of 17.17% and morbidity and mortality both of 35.63% in The Society of Thoracic Surgeons risk calculator, we performed mitral and aortic valve replacement with both biological valves. Postoperative course was complicated with a high dose of inotropic support, cardiac tamponade requiring open drainage, and the need for a tracheostomy. Abdominal distension was observed, and enhanced computed tomography demonstrated free air and a suspected perforated sigmoid colon. Emergency laparotomy revealed a 20-cm longitudinal perforation in the sigmoid colon. A left hemicolectomy with stoma was performed. Immunostaining of a sample of her colon showed cytomegalovirus-positive cells.

Conclusions

Cardiac surgeons should consider cytomegalovirus colitis as a differential diagnosis during the course of cardiac surgery even in immunocompetent patients.
Automated picking of seismic first arrivals using a single- to multidomain self-trained network
Authors: Ozawa Mitsuyuki
Published: August 2023

Over the past few decades, seismic data volume has increased owing to the evolution of survey acquisition designs that comprise numerous receivers and shotpoints. Consequently, the demand for efficient algorithms to detect seismic first arrivals has been increasing. Machine-learning methods, particularly convolutional neural networks, have shown great potential in achieving this. However, most existing methods lack generalization abilities or are too sensitive to noise. We develop a new picking strategy based on a single- to multidomain self-trained network to achieve automated picking of first arrivals. Our self-trained network learns from single to multiple domains while rejecting the incorrectly predicted picks and assigning pseudo-first-arrival times to the unlabeled data set. The model inherits the advantages of single- and multidomain picking strategies and has high generalizability and noise robustness. In experiments on an open hard-rock seismic multisurvey data set, our picking method outperforms the benchmark for most evaluation metrics. Remarkably, our approach achieves an accuracy improvement of approximately 10% against the data from one mine site (Lalor), with a sampling rate that differed from the training data. In addition, our method indicates robust results considering the limited amount of labeled data. The experimental results demonstrate the high generalizability and robustness of our method for unseen data sets and reveals the effectiveness of single- to multidomain self-trained networks to improve the accuracy and stability of automated first-arrival picking. Our method contributes to reducing human labor and the total seismic processing time.
High visual resolution interpretation: The case for virtual seismic reality
Authors: Lynch Steven
Published: August 2023

The twin fields of virtual and augmented reality have revolutionized the gaming and entertainment industries; however, they have had almost no impact on the field of scientific visualization. This is especially true in oil and gas exploration where we continue to visualize seismic data using low visual resolution displays developed in the 1960s and 1970s. Variable density and grayscale displays were a revolution in themselves, allowing us to transition from strictly manual interpretation on paper sections to increasingly automatic interpretations on workstations. This transition was instrumental in allowing us to find the oil necessary to meet the demands of emerging economies. These displays have brought us this far, but they cannot take us into the future. Today, we are exploring for targets whose seismic expression is close to the limits of spatial and temporal resolution and may be below the visual resolution of conventional seismic displays. If we are to meet the current demands of developed economies and the increasing demands of emerging economies, we must replace these, now technologically archaic, low visual resolution displays with high visual resolution displays. For that, we need virtual reality. At its inception, virtual reality was largely ignored by the exploration industry. Today, it has evolved to the point that it could revolutionize scientific visualization, and seismic visualization in particular, as much as it revolutionized gaming and entertainment. I introduce the subject of high visual resolution interpretation and present examples of seismic data in virtual seismic reality.
Seismic Soundoff: A reality check on full-wave inversion
Authors: Geary Andrew
Published: August 2023

Öz Yilmaz returns to the podcast to highlight his award-winning article, “A reality check on full-wave inversion applied to land seismic data for near-surface modeling.”