bluuebunny/crossref_metadata_2025 huggingface dataset:
Allele Sorting as a Novel Approach to Resolving the Origin of Allotetraploids Using Hyb-Seq Data: A Case Study of the Balkan Mountain Endemic Cardamine barbaraeoides
Authors: Šlenker Marek , Kantor Adam , Marhold Karol , Schmickl Roswitha , Mandáková Terezie , Lysak Martin A. , Perný Marián , Caboňová Michaela , Slovák Marek , Zozomová-Lihová Judita
Published: April 2021

Mountains of the Balkan Peninsula are significant biodiversity hotspots with great species richness and a large proportion of narrow endemics. Processes that have driven the evolution of the rich Balkan mountain flora, however, are still insufficiently explored and understood. Here we focus on a group of Cardamine (Brassicaceae) perennials growing in wet, mainly mountainous habitats. It comprises several Mediterranean endemics, including those restricted to the Balkan Peninsula. We used target enrichment with genome skimming (Hyb-Seq) to infer their phylogenetic relationships, and, along with genomic in situ hybridization (GISH), to resolve the origin of tetraploid Cardamine barbaraeoides endemic to the Southern Pindos Mts. (Greece). We also explored the challenges of phylogenomic analyses of polyploid species and developed a new approach of allele sorting into homeologs that allows identifying subgenomes inherited from different progenitors. We obtained a robust phylogenetic reconstruction for diploids based on 1,168 low-copy nuclear genes, which suggested both allopatric and ecological speciation events. In addition, cases of plastid–nuclear discordance, in agreement with divergent nuclear ribosomal DNA (nrDNA) copy variants in some species, indicated traces of interspecific gene flow. Our results also support biogeographic links between the Balkan and Anatolian–Caucasus regions and illustrate the contribution of the latter region to high Balkan biodiversity. An allopolyploid origin was inferred for C. barbaraeoides, which highlights the role of mountains in the Balkan Peninsula both as refugia and melting pots favoring species contacts and polyploid evolution in response to Pleistocene climate-induced range dynamics. Overall, our study demonstrates the importance of a thorough phylogenomic approach when studying the evolution of recently diverged species complexes affected by reticulation events at both diploid and polyploid levels. We emphasize the significance of retrieving allelic and homeologous variation from nuclear genes, as well as multiple nrDNA copy variants from genome skim data.
The Role of Membranes and Lipid-Protein Interactions in the Mg-Branch of Tetrapyrrole Biosynthesis
Authors: Solymosi Katalin , Mysliwa-Kurdziel Beata
Published: April 2021

Chlorophyll (Chl) is essential for photosynthesis and needs to be produced throughout the whole plant life, especially under changing light intensity and stress conditions which may result in the destruction and elimination of these pigments. All steps of the Mg-branch of tetrapyrrole biosynthesis leading to Chl formation are carried out by enzymes associated with plastid membranes. Still the significance of these protein-membrane and protein-lipid interactions in Chl synthesis and chloroplast differentiation are not very well-understood. In this review, we provide an overview on Chl biosynthesis in angiosperms with emphasis on its association with membranes and lipids. Moreover, the last steps of the pathway including the reduction of protochlorophyllide (Pchlide) to chlorophyllide (Chlide), the biosynthesis of the isoprenoid phytyl moiety and the esterification of Chlide are also summarized. The unique biochemical and photophysical properties of the light-dependent NADPH:protochlorophyllide oxidoreductase (LPOR) enzyme catalyzing Pchlide photoreduction and located to peculiar tubuloreticular prolamellar body (PLB) membranes of light-deprived tissues of angiosperms and to envelope membranes, as well as to thylakoids (especially grana margins) are also reviewed. Data about the factors influencing tubuloreticular membrane formation within cells, the spectroscopic properties and thein vitroreconstitution of the native LPOR enzyme complexes are also critically discussed.
Not Too Much and Not Too Little: Information Processing for a Good Purchase Decision
Authors: Vogrincic-Haselbacher Claudia , Krueger Joachim I. , Lurger Brigitta , Dinslaken Isabelle , Anslinger Julian , Caks Florian , Florack Arnd , Brohmer Hilmar , Athenstaedt Ursula
Published: April 2021

When deciding on an online purchase, consumers often face a plethora of information. Yet, individuals consumers differ greatly in the amount of information they are willing and able to acquire and process before making purchasing decisions. Extensively processing all available information does not necessarily promote good decisions. Instead, the empirical evidence suggests that reviewing too much information or too many choice alternatives can impair decision quality. Using simulated contract conclusion scenarios, we identify distinctive types of information processing styles and find that certain search and selection strategies predict the quality of the final choice. Participants (N = 363) chose a cellular service contract in a web-based environment that closely resembled actual online settings in the country of study. Using information processing data obtained with tracking software, we identify three consumer segments differing along two dimensions – the extent dimension, referring to the overall effort invested in information processing, and the focus dimension, referring to the degree to which someone focuses on the best available options. The three subgroups of respondents can be characterized as follows: (1) consumers with a low-effort and low-focus information processing strategy (n = 137); (2) consumers with a moderate-effort and high-focus information processing strategy (n = 124); and (3) consumers with high-effort and low-focus information processing strategy (n = 102). The three groups differed not only in their information processing but also in the quality of their decisions. In line with the assumption of ecological rationality, most successful search strategies were not exhaustive, but instead involved the focused selection and processing of a medium amount of information. Implications for effective consumer information are provided.
Roles of Anxiety and Depression in Predicting Cardiovascular Disease Among Patients With Type 2 Diabetes Mellitus: A Machine Learning Approach
Authors: Chu Haiyun , Chen Lu , Yang Xiuxian , Qiu Xiaohui , Qiao Zhengxue , Song Xuejia , Zhao Erying , Zhou Jiawei , Zhang Wenxin , Mehmood Anam , Pan Hui , Yang Yanjie
Published: April 2021

Cardiovascular disease (CVD) is a major complication of type 2 diabetes mellitus (T2DM). In addition to traditional risk factors, psychological determinants play an important role in CVD risk. This study applied Deep Neural Network (DNN) to develop a CVD risk prediction model and explored the bio-psycho-social contributors to the CVD risk among patients with T2DM. From 2017 to 2020, 834 patients with T2DM were recruited from the Department of Endocrinology, Affiliated Hospital of Harbin Medical University, China. In this cross-sectional study, the patients' bio-psycho-social information was collected through clinical examinations and questionnaires. The dataset was randomly split into a 75% train set and a 25% test set. DNN was implemented at the best performance on the train set and applied on the test set. The receiver operating characteristic curve (ROC) analysis was used to evaluate the model performance. Of participants, 272 (32.6%) were diagnosed with CVD. The developed ensemble model for CVD risk achieved an area under curve score of 0.91, accuracy of 87.50%, sensitivity of 88.06%, and specificity of 87.23%. Among patients with T2DM, the top five predictors in the CVD risk model were body mass index, anxiety, depression, total cholesterol, and systolic blood pressure. In summary, machine learning models can provide an automated identification mechanism for patients at CVD risk. Integrated treatment measures should be taken in health management, including clinical care, mental health improvement, and health behavior promotion.
Play First Before Doing Your Exercise: Does Acting in a Game-Like Task Improve 5-Year-Olds’ Working Memory Performance?
Authors: Fitamen Christophe , Camos Valérie
Published: April 2021

It has been shown that acting in a game-like task improves preschoolers’ working memory when tested in a reconstruction task. The game context and the motor activity during the game would provide goal cues bringing support to the memory processes. The aim of the present study was to test this hypothesis by examining preschoolers’ working memory performance in a game-like task compared to an exercise-like task, which offers less goal cues. In the present study, 5-year-olds had to maintain a series of fruits and vegetables while acting in a game-like task or remaining static during the same task presented in a school-exercise context (within-subject factor). Memory performance was tested either through oral recall or reconstruction of the series of memory items (between-subject factor). Despite the fact that memory performance did not differ between the two conditions (game vs. exercise) whatever the type of memory tests, performance was worst in the game-like than in the exercise condition when the exercise was presented first. No difference emerged between conditions when the game condition was performed first. This result suggests that preschoolers were able to take advantage of acting in the game-like condition to integrate some task requirements, which were beneficial for performing the exercise condition.
Reduced Plasma Dopamine-β-Hydroxylase Activity Is Associated With the Severity of Bipolar Disorder: A Pilot Study
Authors: Sun Zuoli , Bo Qijing , Mao Zhen , Li Feng , He Fan , Pao Christine , Li Wenbiao , He Yi , Ma Xin , Wang Chuanyue
Published: April 2021

Dopamine-β-hydroxylase (DβH) is an enzyme converting dopamine to norepinephrine, a key neurotransmitter in mood disorders, such as major depressive disorder (MDD) and bipolar disorder (BD). Due to overlapping symptomology of unipolar and bipolar depression, the present study attempted to explorer if the plasma DβH activity could discriminate the depressive episodes of BD from MDD. The aim of this study was to compare the plasma DβH activity among MDD patients (n = 104), BD patients (n = 101), and healthy controls (n = 160). Clinical characteristics and cognitive function were assessed using the Young Mania Rating Scale (YMRS), Hamilton Depression Scale (HAM-D), Hamilton Anxiety Scale (HAM-A), Patient Health Questionnaire-9 (PHQ-9), and Repeatable Battery for the Assessment of Neuropsychological Status (RBANS). Our data showed a lower plasma DβH activity in patients with BD, not MDD, than that in controls. For the BD patients, the plasma DβH activities were negatively correlated with HAM-D scores and HAM-A scores. However, there was no significant correlation between plasma DβH activity and severity of depressive symptoms in MDD patients. No significant correlation between DβH activities and cognitive assessments neither in BD nor in MDD patients. The present study provides evidence that BD is associated with decreased circulating DβH activity.
Interaction of Socio-demographic Characteristics on Acceptance of Disability Among Individuals With Physical Disabilities
Authors: Park Eun-Young , Kim Jung-Hee
Published: April 2021

This study aimed to investigate the interaction of sociodemographic characteristics on acceptance of disability among individuals with physical disabilities (IWPD). Data from the 8th Panel Survey of Employment for the Disabled in Korea (PSED) in the second wave were used. A sample concerning the first phase of disability was extracted using the one-step colony method to extract regions and was stratified based on the type of disability, disability grade, and age. To explore the association between acceptance of sociodemographic characteristics and of disability, we used a general linear model. A significant main effect was observed in employment, health status, degree of help, and subjective economic status. Regarding employment status, acceptance of disability in unemployment of IWPD with less than high school was lower as compared to those with more than high school. We observed that unemployed IWPD with low income or poor health status could be the group with the highest risk for acceptance of disability. Individuals in the low economic group were more religious than those in the high economic one. These findings indicate that specialized intervention programs that consider religion, economic status, employment, education, health, and their interactions would be effective for acceptance of disability. Interdisciplinary team members should consider the individual profiles of these populations and implement suitable support and rehabilitation programs.
Toward a Gender-Sensitive Approach of Psychiatric Rehabilitation in Autism Spectrum Disorder (ASD): A Systematic Review of Women Needs in the Domains of Romantic Relationships and Reproductive Health
Authors: Dubreucq Marine , Dubreucq Julien
Published: April 2021

Later age of diagnosis, better expressive behaviors, increased use of camouflage strategies but also increased psychiatric symptoms, more unmet needs, and a general lower quality of life are characteristics often associated with female gender in autism spectrum disorder (ASD). Psychiatric rehabilitation has shown small to moderate effectiveness in improving patients' outcomes in ASD. Few gender differences have been found in the response to psychiatric rehabilitation. This might be related to the predominance of males in research samples, but also to the lack of programs directly addressing women's unmet needs. The objectives of the present paper were: (i) to review the needs for care of autistic women in romantic relationships and reproductive health; (ii) to review the existing psychosocial treatments in these domains; and (iii) to evaluate the strengths and limitations of the current body of evidence to guide future research. A systematic electronic database search (PubMed and PsycINFO), following PRISMA guidelines, was conducted on autistic women's needs for care relating to psychiatric rehabilitation in romantic relationships and reproductive health. Out of 27 articles, 22 reported on romantic relationships and 16 used a quantitative design. Most studies were cross-sectional (n = 21) and conducted in North America or Europe. Eight studies reported on interventions addressing romantic relationships; no published study reported on interventions on reproductive health or parenting. Most interventions did not include gender-sensitive content (i.e., gender variance and gender-related social norms, roles, and expectations). Autistic women and autistic gender-diverse individuals may face unique challenges in the domains of romantic relationships and reproductive health (high levels of stigma, high risk of sexual abuse, increased psychiatric symptoms, and more unmet needs). We discussed the potential implications for improving women's access to psychiatric and psychosocial treatment, for designing gender-sensitive recovery-oriented interventions, and for future research.
Developing and Evaluating a Capacity-Building Intervention for Healthcare Providers to Improve Communication Skills and Awareness of Hard of Hearing and D/deaf Populations: Protocol for a Participative Action Research-Based Study
Authors: Bodenmann Patrick , Singy Pascal , Kasztura Miriam , Graells Madison , Cantero Odile , Morisod Kevin , Malebranche Mary , Smith Pascal , Beyeler Stéphane , Sebaï Tanya , Grazioli Véronique S.
Published: April 2021

Background: D/deaf and hard of hearing populations are at higher risk for experiencing physical and mental health problems compared to hearing populations. In addition, they commonly encounter barriers to accessing and benefiting from health services, which largely stem from challenges they face in communicating with healthcare providers. Healthcare providers commonly lack tailored communication skills in caring for D/deaf and hard of hearing populations, which lead to difficulties and dissatisfaction for both staff and D/deaf and hard of hearing communities. This research project aims to develop and evaluate a capacity-building intervention for healthcare providers with the goal of increasing their awareness of D/deaf and hard of hearing individuals' experiences with the healthcare system, their distinct needs, and improving their capacity to communicate effectively with this patient population.

Methods: This research project features a participative action research design using qualitative and quantitative methods. Consistent with participative action research, the study will actively involve the target populations, key stakeholders and representative associations. The intervention will be developed and tested through iterative phases. The Integrated Model of Training Evaluation and Effectiveness will guide prospective evaluation of the intervention. The latter will involve qualitative and quantitative assessments in participants before and after the intervention and at 6-months follow-up.

Discussion: Results will contribute to research aimed at decreasing barriers to accessing and benefiting from healthcare services for D/deaf and hard of hearing individuals. Findings will be presented to representative associations and political authorities, as well as disseminated at research conferences and in peer-reviewed journals.
Spatially-Resolved Integrated Precipitation-Surface-Groundwater Water Isotope Mapping From Crowd Sourcing: Toward Understanding Water Cycling Across a Post-glacial Landscape
Authors: Cole Alison , Boutt David F.
Published: April 2021

Isotopic analyses of δ18O and δ2H of water in the context of the hydrologic cycle have allowed hydrologists to better understand the portioning of water between the different water domains. Isoscapes on a large spatial scale have been created to show isotopic variation in waters as a function of elevation, temperature, distance to coast, and water vapor source. We present the spatial and temporal isotopic results of precipitation, surface water, and groundwater of an ongoing study across Massachusetts, USA in order to establish an isotopic baseline for the region. This represents one of the most comprehensive and detailed isotopic studies of water across a 10,000 sq mi area that has exhaustively sampled important components of the terrestrial hydrologic cycle (precipitation, groundwater, and surface waters). We leverage the support of volunteers and citizen scientists to crowd source samples for isotopic analysis. The database consists of water samples from 14 precipitation sites, 409 ground water sites and 516 surface water sites across the state of Massachusetts, USA. The results indicate that groundwater isotopic composition ranges from δ18O −11 to −4‰ surface water ranges from δ18O −13 to −3.84‰ and precipitation ranges from δ18O −17.88 to −2.89‰. On a first order, the small bias of mean groundwater (−8.7‰) and surface water (−8.0‰) isotopes compared to precipitation δ18O (−7.6‰) supports that groundwater recharge and surface water storage effects through the hydrologic year impact the isotopic composition of surface and groundwater. While differences are distinct, they are larger than previously reported values, but still suggest more importance of summer precipitation than previously acknowledged. On average seasonal amplitudes of precipitation (2.7‰), surface water (1.13‰), and groundwater (~0‰) of the region demonstrate young water fractions of surface water to be 40% with groundwater ~0%. Results demonstrate that mean δ18O in precipitation, surface water and groundwaters are more enriched in heavy isotopes in areas near the coast, than the interior and western portion of Massachusetts. The hope is for this dataset to become an important tool for water management and water resource assessment across the region.
Effect of Promoter Methylation on the Expression of Porcine MUC2 Gene and Resistance to PEDV Infection
Authors: Xiao Yeyi , Zhou Yajing , Sun Shouyong , Wang Haifei , Wu Shenglong , Bao Wenbin
Published: April 2021

Integrity of the intestinal mucosal barrier is closely related to the occurrence of diarrhea. As an important component protein of the intestinal mucosal barrier, Mucin 2 (MUC2) plays a critical role in preventing the invasion of pathogens, toxins, and foreign bodies. In the present study, we preliminary verified the function of the porcine MUC2 gene in resisting porcine epidemic diarrhea virus (PEDV) infection and investigated the effect of DNA methylation in the promoter region on MUC2 gene expression. The results showed that after PEDV infection, the intestinal mucosal barrier was damaged. Moreover, MUC2 expression was significantly higher in PEDV-infected piglets than in healthy piglets (P < 0.01). The mRNA expression of MUC2 was significantly higher in PEDV-infected IPEC-J2 cells than in non-infected IPEC-J2 cells (P < 0.05). Methylation of the mC-5 site in the MUC2 promoter inhibited the binding of Yin Yang 1 (YY1) to the promoter, down regulated the expression of MUC2 and increased the susceptibility of piglets to PEDV. In conclusion, this study suggests that MUC2 plays an essential regulatory role in PEDV infection. High MUC2 expression improves the resistance of pigs to PEDV infection. The binding of YY1 to the MUC2 promoter is hindered by the methylation of the mC-5 site, which downregulates MUC2 expression and ultimately affects the resistance of pigs to PEDV infection.
International Evidence on the Impact of Health-Justice Partnerships: A Systematic Scoping Review
Authors: Beardon Sarah , Woodhead Charlotte , Cooper Silvie , Ingram Elizabeth , Genn Hazel , Raine Rosalind
Published: April 2021

Background: Health-justice partnerships (HJPs) are collaborations between healthcare and legal services which support patients with social welfare issues such as welfare benefits, debt, housing, education and employment. HJPs exist across the world in a variety of forms and with diverse objectives. This review synthesizes the international evidence on the impacts of HJPs.

Methods: A systematic scoping review of international literature was undertaken. A wide-ranging search was conducted across academic databases and grey literature sources, covering OECD countries from January 1995 to December 2018. Data from included publications were extracted and research quality was assessed. A narrative synthesis approach was used to analyze and present the results.

Results: Reported objectives of HJPs related to: prevention of health and legal problems; access to legal assistance; health improvement; resolution of legal problems; improvement of patient care; support for healthcare services; addressing inequalities; and catalyzing systemic change. There is strong evidence that HJPs: improve access to legal assistance for people at risk of social and health disadvantage; positively influence material and social circumstances through resolution of legal problems; and improve mental wellbeing. A wide range of other positive impacts were identified for individuals, services and communities; the strength of evidence for each is summarized and discussed.

Conclusion: HJPs are effective in tackling social welfare issues that affect the health of disadvantaged groups in society and can therefore form a key part of public health strategies to address inequalities.
Human Cadaveric Artificial Lung Tumor-Mimic Training Model
Authors: Székely Réka , Suhai Ferenc Imre , Karlinger Kinga , Baksa Gábor , Szabaczki Bence , Bárány László , Pölöskei Gergely , Rácz Gergely , Wagner Ödön , Merkely Béla , Ruttkay Tamás
Published: April 2021

Introduction: An important phase in surgical training is gaining experience in real human anatomical situations. When a cadaver is available it may complement the various artificial practice models. However, it is often necessary to supplement the characteristics of the cadavers with a simulation of a tumor. Our objective was to develop an easy-to-create, realistic artificial tumor-mimic model for peripheral lung tumor resection practice.

Methods: In our work we injected barium sulphate enriched silicone suspension into 10 isolated, non-fixed lungs of human cadavers, through the puncture of the visceral pleura. Four lesions–apical, hilar and two peripheral–were created in each of ten specimens. After fixation CT scans were obtained and analyzed. The implanted tumor-mimics were examined after anatomical preparation and slicing. Also performed CT-guided percutaneous puncture was also performed to create the lesions in situ in two lungs of human cadavers.

Results: Analyzing the CT data of 10 isolated lungs, out of 40 lesions, 34 were nodular (85.0%) and in the nodular group five were spiculated (12.5%). Satellite lesions were formed in two cases (5.0%). Relevant outflow into vessels or airway occurred in five lesions (12.5%). Reaching the surface of the lung occured in 11 lesions (27.5%). The tumor-mimics were elastic and adhered well to the surrounding tissue. The two lesions, implanted via percutaneous puncture, both were nodular and one also showed lobulated features.

Conclusion: Our artificial tumor-mimics were easy to create, varied in shape and size, and with percutaneous implantation the lesions provide a model for teaching every step of a surgical procedure.
Spherical One-Way Wave Equation
Authors: Bschorr Oskar , Raida Hans-Joachim
Published: April 2021

The coordinate-free one-way wave equation is transferred in spherical coordinates. Therefore it is necessary to achieve consistency between gradient, divergence and Laplace operators and to establish, beside the conventional radial Nabla operator ∂Φ/∂r, a new variant ∂rΦ/r∂r. The two Nabla operator variants differ in the near field term Φ/r whereas in the far field r≫0 there is asymptotic approximation. Surprisingly, the more complicated gradient ∂rΦ/r∂r results in unexpected simplifications for – and only for – spherical waves with the 1/r amplitude decrease. Thus the calculation always remains elementary without the wattless imaginary near fields, and the spherical Bessel functions are obsolete.
Selenium Modulates Antioxidant Activity, Osmoprotectants, and Photosynthetic Efficiency of Onion under Saline Soil Conditions
Authors: Semida Wael M. , Abd El-Mageed Taia A. , Abdelkhalik Abdelsattar , Hemida Khaulood A. , Abdurrahman Hamdi A. , Howladar Saad M. , Leilah Ahmed A. A. , Rady Mohamed O. A.
Published: April 2021

Selenium (Se) has been recently reported to play a crucial role in ameliorating the negative impact of abiotic stress, including salinity, on several plant species. Two field experiments (2016/17 and 2017/18) were carried out to investigate the possible effect of exogenous Se application at two levels (25, 50 mg L−1) on growth, bulb yield, physio-biochemical attributes, and antioxidant activities of onion grown under saline (5.25 dS m−1) soil condition. Se (25 or 50 mg L−1) foliar application enhanced growth characteristics, as well as membrane stability index (MSI) and relative water content (RWC) as a result of the osmotic adjustment by accumulating more osmoprotectants and enhancing the activity of antioxidants defense system, thus improving photosynthetic efficiency and bulb yield. Proline, glycine betaine, choline, and total soluble sugars content were higher in leaves and bulbs of Se-treated plants under salt stress. Se (25 or 50 mg L−1) significantly increased the activities of enzymatic (ascorbate peroxidase, superoxide dismutase, and catalase) and non-enzymatic (ascorbic acid and glutathione) antioxidants in both leaves and bulbs of salt-stressed onion. Se application at 25–50 mg L−1 may find, in the future, a potential application as anti-abiotic stresses for improving plant growth and productivity under saline soil condition.
The Effect of Diffuse Film Covers on Microclimate and Growth and Production of Tomato (Solanum lycopersicum L.) in a Mediterranean Greenhouse
Authors: Moreno-Teruel María de los Ángeles , Molina-Aiz Francisco Domingo , Peña-Fernández Araceli , López-Martínez Alejandro , Valera-Martínez Diego Luis
Published: April 2021

The efficient use of light is one of the most important factors for the development of greenhouse crops. It is increasingly necessary to use film covers that enhance transmittance and the proportion of diffuse light to generate a more homogeneous light distribution. The objective of this study was to evaluate the effect that an experimental film cover with high transmittance and high light diffusivity produces on the microclimate and the growth and yield of tomato crops (Solanum lycopersicum L.), compared with a commercial thermal film cover. The trial was developed during a spring–summer growing cycle in a multispan greenhouse divided into two compartments (sectors) separated by a vertical polyethylene sheet. In the East sector, a commercial film was installed (transmittance of 85% and diffusivity of 60%) and in the West sector, an experimental film was used (transmittance of 90% and diffusivity of 55%). The results show an increase in the marketable yield of 0.25 kg·m−2 in the sector with the experimental film, which represents 3.2% growth with respect to the commercial film. The photosynthetic activity measured in tomato leaves was 21.5% higher in plants growing in the sector with the experimental film, with had the highest transmittance. The increase in radiation transmittance of 14% produced greater photosynthetic activity without generating a higher inside air temperature at the crop level (at the height of 2 m above the floor). However, the mean temperature of the soil surface was statistically higher on the side with the diffuse experimental cover film, as a logical consequence of the higher level of intercepted solar radiation.
Effects of Oral Hyaluronic Acid Administration in Dogs Following Tibial Tuberosity Advancement Surgery for Cranial Cruciate Ligament Injury
Authors: Serra Aguado Claudio Iván , Ramos-Plá Juan José , Soler Carme , Segarra Sergi , Moratalla Víctor , Redondo José Ignacio
Published: April 2021

Hyaluronic acid (HA) intraarticular injection is used in the management of osteoarthritis in veterinary medicine. However, HA oral administration is less common given the scarce currently available scientific evidence. This study was aimed at evaluating the effects of oral HA administration on synovial fluid concentrations of several selected biomarkers in dogs with cranial cruciate ligament (CCL) injury operated on using the tibial tuberosity advancement (TTA) technique. Fifty-five dogs were included in this prospective, randomized, double-blind, clinical study; they were randomly assigned to receive either a placebo (group A; n = 25) or HA (group B; n = 30) orally for 10 weeks. Synovial fluid samples were obtained before surgery, and at 10 weeks postoperatively to measure concentrations of HA, haptoglobin, nitric oxide, and paraoxonase-1. After 10 weeks, group HA showed a significant increase in HA concentration (p = 0.0016) and a significant decrease in PON-1 concentration (p = 0.011) compared to baseline. In conclusion, post-op oral HA administration in canine patients with CCL injury leads to improvements in osteoarthritis biomarkers, namely higher synovial fluid HA concentrations and reduced synovial fluid paraoxonase-1 concentrations. These findings support the bioavailability of orally-administered HA and its usefulness in improving biomarkers of osteoarthritis.
Nutritive Value, In Vitro Fermentation, and Methane Production of Cactus Cladodes, Sugarcane Bagasse, and Urea
Authors: Siqueira Michelle , Chagas Juana , Monnerat João Paulo , Monteiro Carolina , Mora-Luna Robert , Dubeux José , DiLorenzo Nicolas , Ruiz-Moreno Martin , Ferreira Marcelo
Published: April 2021

This study evaluated the effect of different roughages fed to sheep on nutrient and water intake, ingestive behavior, nitrogen balance, microbial protein synthesis, fermentation parameters, and methane production using an in vitro gas production system. The treatments consisted of five diets: cactus cladodes Nopalea (NUB) and Opuntia (OUB), both with the addition of sugarcane bagasse (SB) and urea/ammonium sulfate (urea/as); Tifton hay (TH); corn silage (CS); and sorghum silage (SS), also with added urea/as. The NUB provided greater (p ≤ 0.03) intakes of dry matter (1024 g/d), digestible organic matter (670 g/d), and crude protein (161 g/d) than those on the SS. The NUB provided greater (p < 0.01) dietary water intake (3023 g/d) than TH. The time spent on rumination was shorter (p < 0.01), and the idle time was longer in animals fed NUB and OUB than TH and CS. Microbial protein synthesis was not affected (p = 0.27). The final pH (6.4) of the incubation fluid and the concentration of NH3-N (39.05 mg/dL) were greater for NUB and OUB. Ruminal parameters and methane production were little or not affected by tested forages. We recommend using cactus cladodes in combination with sugarcane bagasse and urea/as in sheep diets.
Preliminary Clinical and Radiographic Evaluation of a Novel Resorbable Implant of Polylactic Acid (PLA) for Tibial Tuberosity Advancement (TTA) by Modified Maquet Technique (MMT)
Authors: Valiño-Cultelli Victoria , Varela-López Óscar , González-Cantalapiedra Antonio
Published: April 2021

Our objectives were to determine whether PLA implants can be used in TTA with successful results; secondly, to observe whether they provide a faster bone healing; finally, to determine whether weight or age influences bone healing scores. PLA cages were created with a 3D printer. TTA by MMT with PLA implants was performed in 24 patients. Follow-ups were carried out pre-surgical, at 1, 2, and 5 months and consisted of a radiographic study and a lameness assessment. A comparison was performed in terms of weight and age. Patients data, time between follow-up examinations, healing score, and lameness score were compared between patients using commercial software for statistically significant differences p < 0.05. Eighteen dogs finished the study. The ossification degrees presented statistically significant differences between each other. PLA implants maintained the advancement in 100% of cases. Comparing weight and age did not present any statistically significant differences between groups. Lameness presented statistically significant differences between follow-up examinations. Complications were observed in 20.8%. PLA implants for TTA provide good functional results, presenting an acceptable rate of complications. They provide a faster bone healing of the osteotomy gap, which was not affected by age or body weight, and have a clinical recovery time similar to metallic implants.
Spatial Clustering by Red Deer and Its Relevance for Management of Chronic Wasting Disease
Authors: Mysterud Atle , Skjelbostad Isa Nergård , Rivrud Inger Maren , Brekkum Øystein , Meisingset Erling L.
Published: April 2021

Herbivores like cervids usually graze on widely scattered forage, but anthropogenic food sources may cause spatial revisitation and aggregation, posing a risk for transmission of infectious diseases. In 2016, chronic wasting disease (CWD) was first detected in Norway. A legal regulation to ban supplemental feeding of cervids and to fence stored hay bales was implemented to lower aggregation of cervids. Knowledge of further patterns and causes of spatial revisitation can inform disease management. We used a recently developed revisitation analysis on GPS-positions from 13 red deer (Cervus elaphus) to identify the pattern of spatial clustering, and we visited 185 spatial clusters during winter to identify the causes of clustering. Anthropogenic food sources were found in 11.9% of spatial clusters, which represented 31.0% of the clusters in agricultural fields. Dumping of silage and hay bales were the main anthropogenic food sources (apart from agricultural fields), and unfenced hay bales were available despite the regulation. The probability of the clusters being in agricultural fields was high during winter. It may be necessary to find other ways of disposing of silage and enforcing the requirement of fencing around hay bales to ensure compliance, in particular during winters with deep snow.
Anaerobic Digestion and Removal of Sulfamethoxazole, Enrofloxacin, Ciprofloxacin and Their Antibiotic Resistance Genes in a Full-Scale Biogas Plant
Authors: Visca Andrea , Barra Caracciolo Anna , Grenni Paola , Patrolecco Luisa , Rauseo Jasmin , Massini Giulia , Mazzurco Miritana Valentina , Spataro Francesca
Published: April 2021

Anaerobic digestion is one of the best ways to re-use animal manure and agricultural residues, through the production of combustible biogas and digestate. However, the use of antibiotics for preventing and treating animal diseases and, consequently, their residual concentrations in manure, could introduce them into anaerobic digesters. If the digestate is applied as a soil fertilizer, antibiotic residues and/or their corresponding antibiotic resistance genes (ARGs) could reach soil ecosystems. This work investigated three common soil emerging contaminants, i.e., sulfamethoxazole (SMX), ciprofloxacin (CIP), enrofloxacin (ENR), their ARGs sul1, sul2, qnrS, qepA, aac-(6′)-Ib-cr and the mobile genetic element intI1, for one year in a full scale anaerobic plant. Six samplings were performed in line with the 45-day hydraulic retention time (HRT) of the anaerobic plant, by collecting input and output samples. The overall results show both antibiotics and ARGs decreased during the anaerobic digestion process. In particular, SMX was degraded by up to 100%, ENR up to 84% and CIP up to 92%, depending on the sampling time. In a similar way, all ARGs declined significantly (up to 80%) in the digestate samples. This work shows how anaerobic digestion can be a promising practice for lowering antibiotic residues and ARGs in soil.
Exploring the Contribution of the AcrB Homolog MdtF to Drug Resistance and Dye Efflux in a Multidrug Resistant E. coli Isolate
Authors: Schuster Sabine , Vavra Martina , Greim Ludwig , Kern Winfried V.
Published: April 2021

In Escherichia coli, the role of RND-type drug transporters other than the major efflux pump AcrB has largely remained undeciphered (particularly in multidrug resistant pathogens), because genetic engineering in such isolates is challenging. The present study aimed to explore the capability of the AcrB homolog MdtF to contribute to the extrusion of noxious compounds and to multidrug resistance in an E. coli clinical isolate with demonstrated expression of this efflux pump. An mdtF/acrB double-knockout was engineered, and susceptibility changes with drugs from various classes were determined in comparison to the parental strain and its acrB and tolC single-knockout mutants. The potential of MdtF to participate in the export of agents with different physicochemical properties was additionally assessed using accumulation and real-time efflux assays with several fluorescent dyes. The results show that there was limited impact to the multidrug resistant phenotype in the tested E. coli strain, while the RND-type transporter remarkably contributes to the efflux of all tested dyes. This should be considered when evaluating the efflux phenotype of clinical isolates via dye accumulation assays. Furthermore, the promiscuity of MdtF should be taken into account when developing new antibiotic agents.
Tissue Distribution of Oleocanthal and Its Metabolites after Oral Ingestion in Rats
Authors: López-Yerena Anallely , Vallverdú-Queralt Anna , Jáuregui Olga , Garcia-Sala Xavier , Lamuela-Raventós Rosa M. , Escribano-Ferrer Elvira
Published: April 2021

Claims for the potential health benefits of oleocanthal (OLC), a dietary phenolic compound found in olive oil, are based mainly on in vitro studies. Little is known about the tissue availability of OLC, which is rapidly metabolized after ingestion. In this study, the distribution of OLC and its metabolites in rat plasma and tissues (stomach, intestine, liver, kidney, spleen, lungs, heart, brain, thyroid and skin) at 1, 2 and 4.5 h after the acute intake of a refined olive oil containing 0.3 mg/mL of OLC was examined by LC-ESI-LTQ-Orbitrap-MS. OLC was only detected in the stomach and intestine samples. Moreover, at 2 and 4.5 h, the concentration in the stomach decreased by 36% and 74%, respectively, and in the intestine by 16% and 33%, respectively. Ten OLC metabolites arising from phase I and phase II reactions were identified. The metabolites were widely distributed in rat tissues, and the most important metabolizing organs were the small intestine and liver. The two main circulating metabolites were the conjugates OLC + OH + CH3 and OLC + H2O + glucuronic acid, which may significantly contribute to the beneficial health effects associated with the regular consumption of extra virgin olive oil. However, more studies are necessary to determine the concentrations and molecular structures of OLC metabolites in human plasma and tissues when consumed with the presence of other phenolic compunds present in EVOO.
Virgin Olive Oil Phenols, Fatty Acid Composition and Sensory Profile: Can Cultivar Overpower Environmental and Ripening Effect?
Authors: Jukić Špika Maja , Perica Slavko , Žanetić Mirella , Škevin Dubravka
Published: April 2021

The authenticity and typicity of monocultivar oils and knowledge of the changes that environmental olive growing conditions bring to naturally present antioxidants and sensory attributes of virgin olive oils (VOO) are important for quality and safety improvement. This study delivers a comprehensive evaluation of the factors affecting phenolics, fatty acid composition and sensory characteristics of cultivars Oblica and Leccino VOOs throughout ripening season at two distinct olive growing environments during three consecutive crop years, and ranks the importance of each factor. Specified parameters were significantly influenced by olive growing environmental conditions. At the colder location of higher altitude, both cultivars gained higher amount of stearic, linoleic and linolenic fatty acids, as well as a higher proportion of phenolic compounds, but lower amounts of oleic fatty acid. At the warmer location of lower altitude, both cultivars had oils with lower level of fruitiness, bitterness and pungency. Analysis of the main components showed that VOOs were primarily differentiated by the cultivar, then main groups were divided with regard to the growing site, while harvest period affected the biosynthesis of natural VOOs antioxidants but had the least impact. These results reveal that the composition of fatty acids, phenolic content and sensory profile are predominantly characteristics of a cultivar.
The Effect of Environmental pH during Trichothecium roseum (Pers.:Fr.) Link Inoculation of Apple Fruits on the Host Differential Reactive Oxygen Species Metabolism
Authors: Han Zhanhong , Wang Zhenyu , Bi Yang , Zong Yuanyuan , Gong Di , Wang Bin , Li Baojun , Sionov Edward , Prusky Dov
Published: April 2021

Trichothecium roseum is an important postharvest pathogen, belonging to an alkalizing group of pathogens secreting ammonia during fungal growth and colonization of apple fruits. Fungal pH modulation is usually considered a factor for improving fungal gene expression, contributing to its pathogenicity. However, the effects of inoculation with T. roseum spore suspensions at increasing pH levels from pH 3 up to pH 7, on the reactive oxygen species (ROS) production and scavenging capability of the apple fruits, affecting host susceptibility, indicate that the pH regulation by the pathogens also affects host response and may contribute to colonization. The present results indicate that the inoculation of T. roseum spores at pH 3 caused the lowest cell membrane permeability, and reduced malondialdehyde content, NADPH oxidases activity, O2●− and H2O2 production in the colonized fruit. Observations of the colonized area on the 9th day after inoculation at pH 3, showed that the rate of O2●− production and H2O2 content was reduced by 57% and 25%, compared to their activities at pH 7. In contrast, antioxidative activities of superoxide dismutase, catalase and peroxidases of fruit tissue inoculated with spores’ suspension in the presence of a solution at pH 3.0 showed their highest activity. The catalase and peroxidases activities in the colonized tissue at pH 3 were higher by almost 58% and 55.9%, respectively, on the 6th day after inoculation compared to inoculation at pH 7. The activities of key enzymes of the ascorbate-glutathione (AsA-GSH) cycle and their substrates and products by the 9th day after fruit inoculation at pH 3 showed 150%, 31%, 16%, and 110% higher activities of ascorbate peroxidase, monodehydroascorbate reductase, dehydroascorbate reductase and glutathione reductase, respectively, compared to pH 7. A similar pattern of response was also observed in the accumulation of ascorbic acid and dehydroascorbate which showed a higher accumulation at pH 3 compared to the colonization at pH 7. The present results indicate that the metabolic regulation of the pH environment by the T. roseum not only modulates the fungal pathogenicity factors reported before, but it induces metabolic host changes contributing both together to fungal colonization.
Physicochemical, Antioxidant, and Anti-Inflammatory Properties of Rapeseed Lecithin Liposomes Loading a Chia (Salvia hispanica L.) Seed Extract
Authors: Alemán Ailén , Pérez-García Selene , Fernández de Palencia Pilar , Montero María Pilar , Gómez-Guillén María del Carmen
Published: April 2021

Vegetal waste materials were used to produce liposomes with both antioxidant and anti-inflammatory properties. Differences in the chemical composition of rapeseed lecithin (LEC) and a partially purified phospholipid fraction (PPL) were studied in terms of fatty acids (neutral lipids, free fatty acids, and phospholipids), sterols, tocopherols, and amino acid composition. Neutral lipids, campesterol, β-sitosterol, and γ-tocopherol were the most depleted compounds in PPL. Qualitative differences between LEC and PPL were revealed by infrared spectroscopy and differential scanning calorimetry. An ethanol/water antioxidant extract from chia seeds (ChE), with a high content in rosmarinic acid and rosmarinic acid 3-O-glucoside, along with other minor phenolic acids determined by HPLC-MS, was encapsulated in liposomes made of LEC (L-LEC) and PPL (L-PPL) with an entrapment efficiency of 61.3% and 69.3%, respectively. L-PPL suspensions showed smaller particle size and lower ζ potential than their L-LEC counterparts, along with noticeable particle destabilization after 7 days of storage. Antioxidant properties were greater in L-LEC than in L-PPL suspensions. L-LEC, ChE, and lecithin empty liposomes (L-E) showed no cytotoxic effect in either Caco-2 or THP-1 cells and induced downregulation of the inflammation response.