bluuebunny/crossref_metadata_2025 huggingface dataset:
Combined genomic and transcriptomic analysis reveals the contribution of tandem duplication genes to low-temperature adaptation in perennial ryegrass
Authors: Wang Wei , Li Xiaoning , Fan Shugao , He Yang , Wei Meng , Wang Jiayi , Yin Yanling , Liu Yanfeng
Published: July 2023

Perennial ryegrass (Lolium perenne L.) is an agronomically important cool-season grass species that is widely used as forage for ruminant animal production and cultivated in temperate regions for the establishment of lawns. However, the underlying genetic mechanism of the response of L. perenne to low temperature is still unclear. In the present study, we performed a comprehensive study and identified 3,770 tandem duplication genes (TDGs) in L. perenne, and evolutionary analysis revealed that L. perenne might have undergone a duplication event approximately 7.69 Mya. GO and KEGG pathway functional analyses revealed that these TDGs were mainly enriched in photosynthesis, hormone-mediated signaling pathways and responses to various stresses, suggesting that TDGs contribute to the environmental adaptability of L. perenne. In addition, the expression profile analysis revealed that the expression levels of TDGs were highly conserved and significantly lower than those of all genes in different tissues, while the frequency of differentially expressed genes (DEGs) from TDGs was much higher than that of DEGs from all genes in response to low-temperature stress. Finally, in-depth analysis of the important and expanded gene family indicated that the members of the ELIP subfamily could rapidly respond to low temperature and persistently maintain higher expression levels during all low temperature stress time points, suggesting that ELIPs most likely mediate low temperature responses and help to facilitate adaptation to low temperature in L. perenne. Our results provide evidence for the genetic underpinning of low-temperature adaptation and valuable resources for practical application and genetic improvement for stress resistance in L. perenne.
The shaping of onion seedlings performance through substrate formulation and co-inoculation with beneficial microorganism consortia
Authors: Pokluda Robert , Ragasová Lucia Nedorost , Jurica Miloš , Kalisz Andrzej , Komorowska Monika , Niemiec Marcin , Caruso Gianluca , Gąstoł Maciej , Sekara Agnieszka
Published: July 2023

Introduction

Smart management in crop cultivation is increasingly supported by application of arbuscular mycorrhizal fungi (AMF) and plant growth-promoting microorganisms (PGPM), which sustain soil fertility and plant performance. The aim of this study was the evaluation of the effects of consortia composed of (Claroideoglomus claroideum BEG96, Claroideoglomus etunicatum BEG92, Funneliformis geosporum BEG199, Funneliformis mosseae BEG 95, and Rhizophagus irregularis BEG140) and PGPM (Azospirillum brasilense – AZ, or Saccharothrix sp. – S) on onion cultivated in growing media with a composition corresponding to a degraded soil.

Methods

Three types of substrate formulations were used, with peat:sand ratios of 50:50, 70:30, 100:0 (v:v). The analysis of substrate parameters crucial for its fertility (pH, salinity, sorption complex capacity, and elements’ content) and characteristics reflecting onion seedlings’ performance (fresh weight, stress biomarkers, and elements’ content) was performed.

Results

AMF colonized onion roots in all treatments, showing increasing potential to form intercellular structures in the substrates rich in organic matter. Additionally, co-inoculation with PGPM microorganisms accelerated arbuscular mycorrhiza establishment. Increased antioxidant activity and glutathione peroxidase (GPOX) activity of onion roots sampled from the formulations composed of peat and sand in the ratio of 100:0, inoculated with AMF+S, and positive correlation between GPOX, fresh weight and antioxidant activity of onion roots reflected the successful induction of plant acclimatization response. Total phenols content was the highest in roots and leaves of onion grown in substrates with 70:30 peat:sand ratio, and, in the case of roots, it was correlated with AMF colonization parameters but not with antioxidant activity.

Discussion

AMF and PGPM efficiency in supporting onion growth should be linked to the increased onion root system capacity in mineral salts absorption, resulting in more efficient aboveground biomass production. AMF and PGPM consortia were effective in releasing minerals to soluble fraction in substrates rich in organic matter, making elements available for uptake by onion root system, though this phenomenon depended on the PGPM species. Microorganism consortia enhanced onion seedlings’ performance also in substrates with lower content of organic carbon through plant biofertilization and phytostimulation
Advances in the knowledge and therapeutics of schizophrenia, major depression disorder, and bipolar disorder from human brain organoid research
Authors: Villanueva Rosa
Published: July 2023

Tridimensional cultures of human induced pluripotent cells (iPSCs) experimentally directed to neural differentiation, termed “brain organoids” are now employed as an in vitro assay that recapitulates early developmental stages of nervous tissue differentiation. Technical progress in culture methodology enabled the generation of regionally specialized organoids with structural and neurochemical characters of distinct encephalic regions. The technical process of organoid elaboration is undergoing progressively implementation, but current robustness of the assay has attracted the attention of psychiatric research to substitute/complement animal experimentation for analyzing the pathophysiology of psychiatric disorders. Numerous morphological, structural, molecular and functional insights of psychiatric disorders have been uncovered by comparing brain organoids made with iPSCs obtained from control healthy subjects and psychiatric patients. Brain organoids were also employed for analyzing the response to conventional treatments, to search for new drugs, and to anticipate the therapeutic response of individual patients in a personalized manner. In this review, we gather data obtained by studying cerebral organoids made from iPSCs of patients of the three most frequent serious psychiatric disorders: schizophrenia, major depression disorder, and bipolar disorder. Among the data obtained in these studies, we emphasize: (i) that the origin of these pathologies takes place in the stages of embryonic development; (ii) the existence of shared molecular pathogenic aspects among patients of the three distinct disorders; (iii) the occurrence of molecular differences between patients bearing the same disorder, and (iv) that functional alterations can be activated or aggravated by environmental signals in patients bearing genetic risk for these disorders.
Lack of functional brain connectivity was associated with poor inhibition in children with attention-deficit/hyperactivity disorder using near-infrared spectroscopy
Authors: Liao Wenjing , Cao Longfei , Leng Lingli , Wang Shaohua , He Xinyu , Dong Yusang , Yang Rongwang , Bai Guannan
Published: July 2023

Objectives

The present study aimed to evaluate the characteristics of functional brain connectivity in the resting state in children with attention deficit hyperactivity disorder (ADHD) and to assess the association between the connectivity and inhibition function using near-infrared spectroscopy (NIRS).

Methods

In total, 34 children aged 6–13 diagnosed with ADHD were recruited from Hangzhou Seventh People’s Hospital. In comparison, 37 healthy children were recruited from a local primary school as controls matched by age and sex. We used NIRS to collect information on brain images. The Stroop test assessed inhibition function. We compared the differences in functional brain connectivity in two groups by analyzing the resting-state brain network. Pearson partial correlation analysis was applied to evaluate the correlation between functional brain connectivity and inhibition in all the children.

Results

Compared with the control group, results of NIRS images analysis showed that children with ADHD had significantly low functional brain connectivity in regions of the orbitofrontal cortex, left dorsolateral prefrontal cortex, left pre-motor and supplementary motor cortex, inferior prefrontal gyrus, and right middle temporal gyrus (p = 0.006). Inhibition function of children with ADHD was negatively correlated with functional brain connectivity (p = 0.009), while such correlation was not found in the control group.

Conclusion

The present study demonstrated that children with ADHD had relatively low connectivity in several brain regions measured at the resting state. Our results supported the evidence that lack of functional brain connectivity was associated with impaired inhibition function in children with ADHD.
Case report: Mixed dementia associated with autoantibodies targeting the vesicular glutamate transporter 2
Authors: Hansen Niels , Teegen Bianca , Hirschel Sina , Wiltfang Jens , Schott Björn H. , Bartels Claudia , Bouter Caroline
Published: July 2023

Background

Autoantibodies against the vesicular glutamate transporter type 2 (VGlut2) can trigger impaired synaptic signaling and are described here for the first time in association with mixed dementia.

Methods

We report on a 71-year-old female patient with a dementing syndrome who underwent a thorough dementia diagnosis including neuropsychological testing, magnetic resonance imaging (MRI), 18F-fluorodesoxyglucose positron emission tomography (FDG-PET), and a spinal tap to search for neural autoantibodies.

Results

Our patient exhibited mixed dementia. Her CSF revealed elevated ptau 181 protein and a reduced Aß42/40 ratio indicating Alzheimer's disease (AD) pathology. In addition, neuropsychological testing showed a profile consistent with AD with impaired memory, reduced semantic word fluency, naming disorder, and impaired visuoconstructive skills. Nevertheless, in-depth neuropsychological testing also revealed marked psychomotor slowing and visuospatial perceptual impairments that are more indicative of the presence of DLB. Overall, her dementia is more likely of mixed pathology. In addition, we repeatedly detected VGlut2 autoantibodies in her serum.

Conclusion

To the best of our knowledge, this report is the first to describe mixed dementia associated with VGlut2 autoantibodies.
Partial ulnar ostectomy, stereotactic body radiation therapy, and palliative radiation therapy as local limb sparing treatment modalities for ulnar tumors in dogs
Authors: Griffin Maureen A. , Martin Tiffany Wormhoudt , Thamm Douglas H. , Worley Deanna R.
Published: July 2023

Background

Information on dogs that undergo limb preserving local treatment for ulnar tumors is currently limited.

Objective

To describe the clinical characteristics and outcomes in dogs that underwent partial ulnectomy or radiation therapy (RT) for ulnar bone tumors, and to evaluate potential risk factors for outcomes as well as pre-treatment factors for association with treatment modality selected.

Animals

Forty client-owned dogs that underwent partial ulnectomy or RT for an ulnar tumor from July 2006 to July 2021.

Methods

The medical records database from a single institution were retrospectively reviewed, and data were recorded and analyzed.

Results

Radiation therapy was performed in 24 dogs, with 12 stereotactic body RT (SBRT) and 12 palliative RT (PRT) plans, and partial ulnectomy was performed in 16 dogs. Biomechanical complications occurred in 6/12 (50%) dogs that underwent SBRT, 6/12 (50%) dogs that underwent PRT, and 3/16 (18.8%) dogs that underwent ulnectomy. The majority of dogs had a good functional outcome following partial ulnectomy, and no dogs required surgical stabilization of the carpus even with lateral styloid process excision. Pathologic fracture occurred in 4/12 (33.3%) dogs following SBRT and 5/12 (41.7%) dogs following PRT. Local progression or recurrence was documented in 5/12 (41.7%) dogs that underwent SBRT, 2/12 (16.7%) dogs that underwent PRT, and 2/16 (12.5%) dogs that underwent ulnectomy. The overall median survival time was 198 days, and factors that were significantly associated with improved survival time included adjuvant chemotherapy administration and partial ulnectomy as local treatment method for dogs that received chemotherapy.

Clinical relevance

Both RT and ulnectomy were effective and well tolerated local treatment modalities for dogs with ulnar tumors.
Mathematical Model of Fuse Effect Initiation in Fiber Core
Authors: Starikova Victoria A. , Konin Yuri A. , Petukhova Alexandra Yu. , Aleshkina Svetlana S. , Petrov Andrey A. , Perminov Anatolii V.
Published: July 2023

This work focuses on the methods of creating in-fiber devices, such as sensors, filters, and scatterers, using the fiber fuse effect. The effect allows for the creation of structures in a fiber core. However, it is necessary to know exactly how this process works, when the plasma spark occurs, what size it reaches, and how it depends on external parameters such as power and wavelength of radiation. Thus, this present study aims to create the possibility of predicting the consequences of optical breakdown. This paper describes a mathematical model of the optical breakdown initiation in a fiber core based on the thermal conductivity equation. The breakdown generates a plasma spark, which subsequently moves along the fiber. The problem is solved in the axisymmetric formulation. The computational domain consists of four elements with different thermophysical properties at the boundaries of which conjugation conditions are fulfilled. The term describing the heat source in the model is determined by the wavelength of radiation and the refractive indices of the core and the shell and also includes the radiation absorption on the released electrons during the thermal ionization of the quartz glass. The temperature field distributions in the optical fiber are obtained. Based on the calculations, it is possible to estimate the occurrence times of various phase states inside the fiber, in particular, the plasma spark occurrence time.
A Survey of Optimal Control Allocation for Aerial Vehicle Control
Authors: Blaha Till Martin , Smeur Ewoud Jan Jacob , Remes Bart Diane Walter
Published: July 2023

In vehicle control, control allocation is often used to abstract control variables from actuators, simplifying controller design and enhancing performance. Surveying available literature reveals that explicit solutions are restricted to strong assumptions on the actuators, or otherwise fail to exploit the capabilities of the actuator constellation. A remedy is to formulate hierarchical minimization problems that take into account the limits of the actuators at the expense of a longer computing time. In this paper, we compared the most common norms of the objective functions for linear or linearized plants, and show available numeric solver types. Such a comparison has not been found in the literature before and indicates that some combinations of linear and quadratic norms are not sufficiently researched. While the bulk of the review is restricted to control-affine plant models, some extensions to dynamic and nonlinear allocation problems are shown. For aerial vehicles, a trend toward linearized incremental control schemes is visible, which forms a compromise between real-time capabilities and the ability to resolve some nonlinearities common in these vehicles.
Management Control Practices as Performance Facilitators in a Crisis Context
Authors: Martins Adelaide , Oliveira Cidália , Silva Rui , Castelo Branco Manuel
Published: July 2023

In modern times, disruptive contexts have challenged the functioning of organisations, as shown by recent events, such as the COVID-19 pandemic and the current war in Europe. The literature highlights the role of management control practices (MCP) as resources that help to improve decision-making processes and organisational performance and competitiveness in a crisis context. In response to stakeholders’ pressures, companies are integrating Corporate Social Responsibility (CSR) issues into their measurement and control systems. The aim of this research is to examine whether companies perceive MCP as a resource to support the decision-making process and contribute to organisational performance and competitiveness in a crisis environment, as well as to ascertain the perceived role of CSR in MC. A survey by questionnaire was conducted among the 250 major exporting companies in Portugal. Results show that in a crisis context of uncertainty and unpredictability such as the COVID-19 pandemic organisations perceive MCP as having (1) a significant influence on organisational performance and on leveraging organisational competitiveness; (2) a significant link with CSR. In general terms, this study provides new insights into the perceptions of the role of MCP as a valuable resource to achieve organisational competitiveness and performance in disruptive social and economic contexts.
Experimental Investigations on Flow Control of the Rotor via the Synthetic Jets in Forward Flight
Authors: Chen Xi , Ding Yan , Zhao Guoqing , Zhao Qijun
Published: July 2023

To study the effects of synthetic jet control on the aerodynamic performance of a rotor in forward flight, we conducted a series of experiments with varying rotor rotation speeds and free stream velocities. In the test, we used a six-component balance and a PIV system and designed a blade with a particular structure that covered the frame. The experimental results revealed that the synthetic jet could effectively delay flow separation over the blade and enhance the aerodynamic efficiency of the rotor. Moreover, we investigated how different jet parameters influenced the flow control effects of synthetic jets on the rotor’s aerodynamic characteristics. We drew some valuable conclusions from our analysis. In forward flight, the jet located closer to the leading edge of the blade had a stronger impact on improving the aerodynamic performance of the rotor. The jet with a 90° jet angle increased the rotor normal force by 225%, which was the maximum possible increase, while the jet with a 30° inclined angle had the best control effects on preventing flow separation in the retreating blade. Our study provides valuable insights into the use of synthetic jets for rotor flow control and suggests possible applications for improving rotorcraft performance and stability.
The Selection of an Energy-Saving Engine Mode Based on the Power Delivery and Fuel Consumption of a 95 kW Tractor during Rotary Tillage
Authors: Siddique Md. Abu Ayub , Baek Seung-Yun , Baek Seung-Min , Jeon Hyeon-Ho , Lee Jun-Ho , Son Mo-A , Yoon Su-Young , Kim Yong-Joo , Lim Ryu-Gap
Published: July 2023

The objective of this study was to estimate power delivery efficiency and fuel consumption based on engine modes. In this study, a 95 kW power-shift tractor was used to analyze power delivery and estimate fuel consumption during rotary tillage. Rotary tillage was conducted in a field experiment with the conventional, APS (auto power shift) power, and APS ECO engine modes. To analyze the field conditions, the soil hardness and soil water content were measured, and soil samples were collected from the experimental site to analyze the soil texture by using the USDA soil texture triangle. Finally, an efficient and suitable engine mode was selected for rotary tillage based on the working load. It was observed that the power delivery and tractive efficiencies when using the APS power mode were the highest among other engine modes, accounting for around 89.23 and 73.45%, respectively. However, the fuel consumption when using the APS power mode was approximately 23.02 L/h, which was also comparatively higher than that of the other engine modes. Additionally, the tractive efficiencies of each engine mode were compared using the Brixius prediction model. The statistical analysis of the predicted tractive efficiencies and those in the tests showed that there were no significant differences among the engine modes; this indicates that the APS controller could perform with high accuracy. In the conventional mode, the power delivery, tractive efficiency, and fuel consumption were approximately 66.48%, 55.89%, and 17.04 L/h, respectively, which were comparatively low. However, the slip ratio in the conventional mode was 18.80%, which was higher than that in the APS power and APS ECO modes. On the other hand, PDE, TE, and fuel consumption when using APS ECO were around 77.57%, 58.44%, and 19.39 L/h, respectively, which were higher than those of the conventional mode, but lower than those of the APS power mode. Furthermore, the comparative analysis showed that the working loads in the APS ECO mode were located in the ungoverned region and were very close to the engine’s maximum torque, which could allow sudden and unwanted engine turn-off due to the fluctuations in working loads, which is to be avoided. The fuel consumption was also comparatively low. However, the working loads in the conventional and APS power modes were located in the governed region, which was outside the engine’s operating range. Therefore, we recommend that users operate tractors in the APS ECO engine mode for rotary tillage, considering fuel economics and high working loads.
Design and Experimental Study of Ball-Head Cone-Tail Injection Mixer Based on Computational Fluid Dynamics
Authors: Shi Yixin , Xiang Siliang , Xu Minzi , Huang Defan , Liu Jianfei , Zhang Xiaocong , Jiang Ping
Published: July 2023

The uniform and accurate mixing of pesticides in water is a necessary prerequisite for plant protection, especially for enabling precise variable spraying, and is also an important method to achieve a precise reduction in pesticide spraying. In order to ensure the uniform mixing of pesticides and water and solve the problems of traditional injection mixers, such as the limited range in the mixing ratio and unadjustable proportion, an active injection liquid mixer is designed in this paper. The mixer can be matched with an online mixing and spraying device to achieve accuracy in mixing and spraying. In this paper, a computational fluid dynamics (CFD) method is used to optimize the structure of the mixer. Through comparative analysis, the optimal structure of the mixer was found. It has a spherical head and conical tail, the number of guide plates is seven, and the shape is semicircular. By calculating the volume fraction of pesticide distribution under different cross-sections, the coefficient of variation in the process of mixing is obtained. The analysis shows that the maximum coefficient of variation of the ball-head cone-tail active injection mixer was 2.88% (lower than the allowable 5%) with a mixing ratio ranging from 300:1 to 3000:1. At the same time, image analysis methods of high-definition photography and ultraviolet spectrophotometry were used to analyze the mixing effect of the mixer. The test results show that, when the pressure of the pesticide injection is 1 MPa, the distribution of the pesticide and water in the ball-head cone-tail injection mixer is more uniform under different mixing ratios, and it has a better spatio-temporal distribution uniformity with the concentration changing a little at different times and different spatial locations. The mixer can provide a theoretical reference and technical support for the subsequent realization of an accurate online variable spray.
Knocking out OsNAC050 Expression Causes Low-Temperature Tolerance in Rice by Regulating Photosynthesis and the Sucrose Metabolic Pathway
Authors: Wang Bo , Wang Yiheng , Xie Likun , Yu Wancong , Lan Qingkuo , Wang Yong , Chen Chengbin , Zhang Yong
Published: July 2023

Because rice is native to tropical and subtropical regions, it is generally more sensitive to cold stress compared to other cereals. Low-temperature stress has a strong influence on the growth, development, and yield of rice. Plant NAM, ATAF1/2 and CUC(NAC) transcription factors (TFs) play significant regulatory roles in stress response. In our research, we found that OsNAC050 (LOC_Os03g60080) is mainly expressed in leaves. Cold (4 °C), heat (42 °C), PEG 6000 (20%, w/v), NaCl (200 mM), H2O2 (1%), IAA (100 μM), ABA (100 μM) and GA3 (100 μM) were used to treat wild type (WT) plants. A low temperature further up-regulated OsNAC050 expression. OsNAC050 mutants created using CRISPR-Cas9 gene editing technology showed significantly enhanced tolerance to the low-temperature treatment. The measurement of enzyme activities related to the redox pathway also showed that mutants have stronger viability under low-temperature stress. Comparative transcriptome analysis showed that photosynthesis and soluble sugar metabolism were significantly affected in OsNAC050 mutant lines, suggesting that OsNAC050 may participate in the above molecular pathways in response to low-temperature stress. The results expand our understanding of the molecular mechanisms underlying the responses to cold stress in rice and can provide new strategies for engineering cold tolerance in high-yielding rice varieties.
Soil Quality Assessment in Response to Water Erosion and Mining Activity
Authors: Vaca Rocio , Del Águila Pedro , Yañez-Ocampo Gustavo , Lugo Jorge A. , De la Portilla-López Nadia
Published: July 2023

Erosion significantly decreases the depth of a soil, the nutrients available for plants, the organic matter and, consequently, the productivity of the edaphic environment. Due to the above considerations, the objective of this study was to evaluate, through various properties, the quality of two eroded soils, one eroded by water and the other by mining activity, amended with biosolids. The quality for both soils was estimated through the selection of a minimum set of data by means of principal component analysis (PCA) and the subsequent realization of correlations, multiple regressions and finally calculations of normalized values (Vn) of those properties considered as indicators of soil quality. According to the results, inorganic nitrogen (NI) and respiratory activity (RA) were the properties selected as indicators to assess quality. For soil eroded by water and by mining activity, NI presented a low and very low quality, respectively (class 4 and 5 of quality according to the calculation of Vn). The quality of RA in soil eroded by mining extraction was very high (quality class 1 according to Vn), and thus it can be considered an ideal indicator for the evaluation of soil quality due to its sensitivity to anthropogenic changes (mining) in soil.
Grain Yield of Maize Crops under Nitrogen Fertigation Using Wastewater from Swine and Fish Farming
Authors: Alves Daniely Karen Matias , Teixeira Marconi Batista , Cunha Fernando Nobre , Cabral Filho Fernando Rodrigues , Cunha Gabriela Nobre , Andrade Christiano Lima Lobo de
Published: July 2023

Maize is one of the most important cereals in the world. It is a crop demanding in nitrogen. Therefore, alternative sources of fertilization bring agronomic, environmental and economic benefits. The objective of this study was to evaluate the biomass and productivity of maize fertigated with wastewater from swine and fish farming in different dilutions. The soil used is classified as Dystroferric Red Latosol, Cerrado phase, with clayey texture. The experimental design used was randomized blocks in 2 × 4 split plots with three replications. The treatments consisted of two sources of wastewater (fish farming and swine farming) diluted with the recommended dose of wastewater + 0, 25, 50 and 75% of its volume in water. The accumulation of dry mass (leaf, stalk and aerial parts) was evaluated at 30, 60, 90 and 110 days after sowing. The evaluation of yield variables (number of grain rows, number of grains per row, grain sizes, grain dry weight, gran yield and harvest index) occurred at 130 days after sowing. The largest accumulation of dry biomass at the end of the maize cycle and evaluated productivity variables were obtained with the application of swine farming wastewater. Wastewater from fish farming applied via an irrigation system without dilution (0%) is the most suitable for obtaining the productivity of corn grains. The two sources of wastewater have the potential to partially replace mineral nitrogen fertilizer in maize.
Antimicrobial Resistance Profiles, Virulence Determinants, and Biofilm Formation in Enterococci Isolated from Rhesus Macaques (Macaca mulatta): A Potential Threat for Wildlife in Bangladesh?
Authors: Ferdous Farhana Binte , Islam Md. Saiful , Ullah Md. Ashek , Rana Md. Liton , Punom Sadia Afrin , Neloy Fahim Haque , Chowdhury Mohammad Nizam Uddin , Hassan Jayedul , Siddique Mahbubul Pratik , Saha Sukumar , Rahman Md. Tanvir
Published: July 2023

Enterococci are commensal bacteria that inhabit the digestive tracts of animals and humans. The transmission of antibiotic-resistant genes through human–animal contact poses a potential public health risk worldwide, as zoonoses from wildlife reservoirs can occur on every continent. The purpose of this study was to detect Enterococcus spp. in rhesus macaques (Macaca mulatta) and to investigate their resistance patterns, virulence profiles, and biofilm-forming ability. Conventional screening of rectal swabs (n = 67) from macaques was followed by polymerase chain reaction (PCR). The biofilm-forming enterococci were determined using the Congo red agar plate assay. Using the disk diffusion test (DDT), antibiogram profiles were determined, followed by resistance and virulence genes identification by PCR. PCR for bacterial species confirmation revealed that 65.7% (44/67) and 22.4% (15/67) of the samples tested positive for E. faecalis and E. faecium, respectively. All the isolated enterococci were biofilm formers. In the DDT, enterococcal isolates exhibited high to moderate resistance to penicillin, rifampin, ampicillin, erythromycin, vancomycin, and linezolid. In the PCR assays, the resistance gene blaTEM was detected in 61.4% (27/44) of E. faecalis and 60% (9/15) of E. faecium isolates. Interestingly, 88.63 % (39/44) of E. faecalis and 100% (15/15) of E. faecium isolates were phenotypically multidrug-resistant. Virulence genes (agg, fsrA, fsrB, fsrC, gelE, sprE, pil, and ace) were more frequent in E. faecalis compared to E. faecium; however, isolates of both Enterococcus spp. were found negative for the cyl gene. As far as we know, the present study has detected, for the first time in Bangladesh, the presence of virulence genes in MDR biofilm-forming enterococci isolated from rhesus macaques. The findings of this study suggest employing epidemiological surveillance along with the one-health approach to monitor these pathogens in wild animals in Bangladesh, which will aid in preventing their potential transmission to humans.
Genetic Determinants of Acinetobacter baumannii Serum-Associated Adaptive Efflux-Mediated Antibiotic Resistance
Authors: Young Mikaeel , Chojnacki Michaelle , Blanchard Catlyn , Cao Xufeng , Johnson William L. , Flaherty Daniel , Dunman Paul M.
Published: July 2023

Acinetobacter baumannii is a nosocomial pathogen of serious healthcare concern that is becoming increasingly difficult to treat due to antibiotic treatment failure. Recent studies have revealed that clinically defined antibiotic-susceptible strains upregulate the expression of a repertoire of putative drug efflux pumps during their growth under biologically relevant conditions, e.g., in human serum, resulting in efflux-associated resistance to physiologically achievable antibiotic levels within a patient. This phenomenon, termed Adaptive Efflux Mediated Resistance (AEMR), has been hypothesized to account for one mechanism by which antibiotic-susceptible A. baumannii fails to respond to antibiotic treatment. In the current study, we sought to identify genetic determinants that contribute to A. baumannii serum-associated AEMR by screening a transposon mutant library for members that display a loss of the AEMR phenotype. Results revealed that mutation of a putative pirin-like protein, YhaK, results in a loss of AEMR, a phenotype that could be complemented by a wild-type copy of the yhaK gene and was verified in a second strain background. Ethidium bromide efflux assays confirmed that the loss of AEMR phenotype due to pirin-like protein mutation correlated with reduced overarching efflux capacity. Further, flow cytometry and confocal microscopy measures of a fluorophore 7-(dimethylamino)-coumarin-4-acetic acid (DMACA)-tagged levofloxacin isomer, ofloxacin, further verified that YhaK mutation reduces AEMR-mediated antibiotic efflux. RNA-sequencing studies revealed that YhaK may be required for the expression of multiple efflux-associated systems, including MATE and ABC families of efflux pumps. Collectively, the data indicate that the A. baumannii YhaK pirin-like protein plays a role in modulating the organism’s adaptive efflux-mediated resistance phenotype.
Antimicrobial Efficacy of 7-Hydroxyflavone Derived from Amycolatopsis sp. HSN-02 and Its Biocontrol Potential on Cercospora Leaf Spot Disease in Tomato Plants
Authors: Math Halaswamy Hire , Kumar Raju Suresh , Chakraborty Bidhayak , Almansour Abdulrahman I. , Perumal Karthikeyan , Kantli Girish Babu , Nayaka Sreenivasa
Published: July 2023

The actinomycete strain HSN-02 was isolated from the soil of a mining field in the Sandur region, Bellary, Karnataka, India. According to the morphological, cultural, physiological, and biochemical characteristics and the 16S rDNA sequence analysis, the strain HSN-02 was identified as Amycolatopsis sp. The antimicrobial activity strain HSN-02 presented stable and moderate inhibitory activity against human pathogens. In pot experiments in the greenhouse, the development of Cercospora leaf spot was markedly suppressed by treatment with the purified compound from the strain HSN-02, and the control efficacy was 45.04 ± 1.30% in Septoria lycopersici-infected tomato plants. A prominent compound was obtained from the fermentation broth of the strain HSN-02 using column chromatography and HPLC. The chemical structural analyses using UV, FTIR, HR-ESI-MS, and NMR confirmed that the compound produced by the strain HSN-02 is 7-hydroxyflavone. This investigation showed the role which the actinomycete strain can play in controlling leaf spots caused by S. lycopersici to reduce treatments with chemical fungicides.
A Scoping Review of Antimicrobial Usage and Antimicrobial Resistance in Beef Cow–Calf Herds in the United States and Canada
Authors: Wilhelm Barbara , Fossen Jayce , Gow Sheryl , Waldner Cheryl
Published: July 2023

Background: The magnitude and knowledge gaps regarding antimicrobial use (AMU) and antimicrobial resistance (AMR) have not been summarized for the North American cow–calf production sector, although estimates of AMU and AMR are essential to AMR risk analysis. The objectives of this scoping review were to map AMU and AMR in the beef cow–calf sector in Canada and the United States, summarize published AMU/AMR predictors, and identify research gaps. Methods: An electronic search was conducted of four bibliographic databases and Google Scholar, augmented by a hand-search of captured studies. Results: Twenty-three of three-hundred and forty-three publications screened advanced to data extraction. Of these, 10 were conducted in the USA and 13 in Canada. Thirteen studied AMR and twelve studied AMU, with two reporting both. Of twelve captured AMU studies, nine presented counts of herd AMU by antimicrobial class or specific antimicrobial. Antimicrobial resistance in Escherichia coli (E. coli) was reported in nine studies. Risk factors for AMU include herd size, vaccine use, and start date of calving season. Conclusions: Overall, a small number of AMR studies were available for synthesis in primarily one population (cows) reporting E. coli AMR. Additional studies targeting reasons for AMU in calves, the impact of management procedures on AMU, potential environmental AMR sources, and AMR in respiratory pathogens and enteric organisms other than E. coli for pre-weaning calves are required to inform AMR risk mitigation strategies.
Multi-Attribute NMS: An Enhanced Non-Maximum Suppression Algorithm for Pedestrian Detection in Crowded Scenes
Authors: Wang Wei , Li Xin , Lyu Xin , Zeng Tao , Chen Jiale , Chen Shangjing
Published: July 2023

Removing duplicate proposals is a critical process in pedestrian detection, and is usually performed via Non-Maximum Suppression (NMS); however, in crowded scenes, the detection proposals of occluded pedestrians are hard to distinguish from duplicate proposals, making the detection results inaccurate. In order to address the above-mentioned problem, the authors of this paper propose a Multi-Attribute NMS (MA-NMS) algorithm, which combines density and count attributes in order to adaptively adjust suppression, effectively preserving the proposals of occluded pedestrians while removing duplicate proposals. In order to obtain the density and count attributes, an attribute branch (ATTB), which uses a context extraction module (CEM) to extract the context of pedestrians, and then, concatenates the context with the features of pedestrians in order to predict both the density and count attributes simultaneously, is also proposed. With the proposed ATTB, a pedestrian detector, based on MA-NMS, is constructed for pedestrian detection in crowded scenes. Extensive experiments are conducted using the CrowdHuman and CityPersons datasets, and the results show that the proposed method outperforms mainstream methods on AP (average precision), Recall, and MR−2 (log-average miss rate), sufficiently validating the effectiveness of the proposed MA-NMS algorithm.
Design and Low-Temperature Performance Evaluation of High-Modulus Co-Modified Asphalt Mixes with Rock Asphalt/Rubber Powder
Authors: Wang Lianfang , Sun Lijun , Lv Quan
Published: July 2023

High-modulus asphalt mixes are effective means to solve rutting problems, but they perform poorly at low temperatures. This study aims to enhance the modulus and low-temperature properties of mixes. Firstly, composite-modified asphalts and mixes were prepared by incorporating rubber powder and rock asphalt. Secondly, their mechanical and viscoelastic properties were investigated to determine the appropriate mass ratios of rubber powder and rock asphalt in asphalt to be 20% and 6%, respectively. The results show that both rock asphalt and rubber powder can enhance the softening point and viscosity of basic asphalt while reducing penetration. Furthermore, their combination significantly improves the high-temperature performance of the material. It is noteworthy that the rubber powder also improves the weakening of rock asphalt for mixtures at low temperatures. Finally, this study employs dynamic and static modulus tests, rutting tests, and beam bending tests to clarify the road properties of composite-modified asphalt mixes. The results indicate that mixes have high-modulus and water damage resistance while considering acceptable low-temperature performance. This paper not only enhances the adaptability of high-modulus asphalt in different environments but also expands its application range.
Detection of Human Visceral Leishmaniasis Parasites in Microscopy Images from Bone Marrow Parasitological Examination
Authors: Gonçalves Clésio , Borges Armando , Dias Viviane , Marques Júlio , Aguiar Bruno , Costa Carlos , Silva Romuere
Published: July 2023

Visceral Leishmaniasis (VL) is a neglected disease that affects between 50,000 and 90,000 new cases annually worldwide. In Brazil, VL causes about 3500 cases/per year. This chronic disease can lead to death in 90% of untreated cases. Thus, it is necessary to study safe technologies for diagnosing, treating, and controlling VL. Specialized laboratories carry out the VL diagnosis, and this step has a significant automation power through methods based on computational tools. The gold standard for detecting VL is the microscopy of material aspirated from the bone marrow to search for amastigotes. This work aims to assist in detecting amastigotes from microscopy images using deep learning techniques. The proposed methodology consists of segmenting the Leishmania parasites in the images, precisely indicating the location of the amastigotes in the image. In the detection of VL parasites, in this methodology, a Dice of 80.4% was obtained, Intersection over Union (IoU) of 75.2%, Accuracy of 99.1%, Precision of 81.5%, Sensitivity of 72.2%, Specificity of 99.6%, and Area under the Receiver Operating Characteristics Curve (AUC) of 86.5%. The results are promising and demonstrate that deep learning models trained with images of microscopy slides of biological material can precisely help the specialist detect VL in humans.
Estimation of Height Changes of Continuous GNSS Stations in the Eastern Anatolia Region during the Seasonal Variation
Authors: Tekin Ünlütürk Nihal Tekin , Doğan Uğur
Published: July 2023

Estimating the height component of Global Navigation Satellite System (GNSS) stations is widely known to be more challenging than estimating the horizontal position. In this study, we utilized height time series data from 37 continuous GNSS stations that were part of the Turkish RTK CORS Network called TUSAGA-Active (Turkish National Permanent GNSS Network Active). The data covered the period from 2014 to 2019, and the selection of stations focused on the Eastern Anatolia region of Turkey due to its topographic characteristics and the pronounced influence of seasonal changes, which facilitated the interpretation of the effects on the height component. The daily coordinates of the GNSS stations were derived using the GAMIT/GLOBK software solution. We identified statistically significant trends, periodic variations, and stochastic components associated with the stations by applying time series analysis to these daily coordinate values. As a result, the vertical velocities of the GNSS stations were determined, along with their corresponding standard deviations. Furthermore, examining the height components of the continuous GNSS stations revealed seasonal effects. We aimed to investigate the potential relationship between these height components and meteorological parameters. The study provides evidence of the interconnectedness between the height components of continuous GNSS stations and various meteorological parameters. Simple linear regression analysis and ARMA time series modeling were utilized to establish this relationship.
Artificial Neural Network Model to Predict Final Construction Contract Duration
Authors: Alsugair Abdullah M. , Al-Gahtani Khalid S. , Alsanabani Naif M. , Alabduljabbar Abdulmajeed A. , Almohsen Abdulmohsen S.
Published: July 2023

Forecasting the final construction contract duration at an early stage plays a vital role in the progress of a project. An inaccurate project duration prediction may lead to the project’s benefits being lost. It is essential to precisely predict the duration due to the presence of several different factors. This paper contributed to developing a model to predict final construction contract duration (FCCD) in the early stages based on parameters characterized as few and shared for any contract. (contract cost, contract duration, and sector). This paper developed an Artificial Neural Network (ANN) model based on 135 Saudi construction project data. The development model has three stages. The first stage was standardization and augmentation using Zavadskas and Turskis’ logarithmic and Pasini methods. The second and third stages were the first and second analyses of the ANN models, respectively. The first analysis aimed to promote the used data and integrate them into the second analysis to develop the ANN model. The ANN models were compared with three linear regression (LR) models (LR1, LR2, and LR3) and other models in the literature. The results revealed that the accuracy of the ANN model provides reasonable accuracy with an average mean absolute percentage error (MAPE) of 12.22%, which is lower than the LR3′s MAPE by 27.03%. The accuracy of the ANN model is similar to that of earned value management (EVM) in the previous study. This paper supports research to deal with relatively little data and integrate them into a neural network. The ANN model assists the stakeholder in making appropriate decisions for the project during the pre-tendering phase by predicting the actual contract duration based on the CC, CD, and project sector.
Prevalence and Distribution of Thermotolerant Campylobacter Species in Poultry: A Comprehensive Review with a Focus on the Factors Affecting the Detection and Enumeration of Campylobacter jejuni and Campylobacter coli in Chicken Meat
Authors: Andritsos Nikolaos D. , Tzimotoudis Nikolaos , Mataragas Marios
Published: July 2023

It is well known that the strong-evidence foodborne outbreaks of human campylobacteriosis are associated with the consumption of raw or incompletely thermally processed poultry meat, whereas broilers act as the main reservoir for Campylobacter species. Campylobacter jejuni and Campylobacter coli are the two main species of campylobacters detected in chicken meat, while they account for almost 90% of the reported cases of campylobacteriosis in humans. Over 80% of these cases are attributed to C. jejuni and about 10% of them are due to C. coli. Therefore, until recently the dominance of C. jejuni against all other Campylobacter spp. isolated from chicken meat samples was well-established and unquestionable. Lately, however, C. coli has been increasingly recovered from chicken meat to such an extent that it is now evident that it often comprises the dominant species among the identified campylobacters in the meat samples. This work attempts for the first time a detailed review of the literature to deepen into this noteworthy epidemiological swift in the prevalence of C. jejuni and C. coli, along with the distribution of Campylobacter spp. in chicken meat. Factors such as the sampling method followed for screening campylobacters in broiler carcasses (e.g., swabs or carcass rinsates, skinned or skinless meat excised samples) and part of the animal carcass from which the sample is obtained (e.g., neck, breast, leg), seasonality of sampling (summer vs. winter) and environmental conditions (e.g., rainfall, relative humidity) at the farm level, the isolation procedure (enumeration or detection) and pathogen identification (biochemical or molecular), the enrichment and plating isolation media (e.g., Bolton vs. Preston broth, charcoal-based vs. chromogenic agars), as well as the biofilm-forming ability of different campylobacters, highlight the multivariate dimension of the phenomenon and are thoroughly discussed in the present review.