bluuebunny/crossref_metadata_2025 huggingface dataset:
Convex Stochastic Approaches for the Optimal Allocation of Distributed Energy Resources in AC Distribution Networks with Measurements Fitted to a Continuous Probability Distribution Function
Authors: Mendoza Osorio Diego , Rosero Garcia Javier
Published: July 2023

This paper addresses the optimal stochastic allocation of distributed energy resources in distribution networks. Typically, uncertain problems are analyzed in multistage formulations, including case generation routines, resulting in computationally exhaustive programs. In this article, two probabilistic approaches are proposed–range probability optimization (RPO) and value probability optimization (VPO)–resulting in a single-stage, convex, stochastic optimal power flow problem. RPO maximizes probabilities within a range of uncertainty, whilst VPO optimizes the values of random variables and maximizes their probabilities. Random variables were modeled with hourly measurements fitted to the logistic distribution. These formulations were tested on two systems and compared against the deterministic case built from expected values. The results indicate that assuming deterministic conditions ends in highly underestimated losses. RPO showed that by including ±10% uncertainty, losses can be increased up to 40% with up to −72% photovoltaic capacity, depending on the system, whereas VPO resulted in up to 85% increases in power losses despite PV installations, with 20% greater probabilities on average. By implementing any of the proposed approaches, it was possible to obtain more probable upper envelopes in the objective, avoiding case generation stages and heuristic methods.
Acoustic Triggering of Combustion Instability in a Swirling Flame: An Experimental Study
Authors: Liu Yunpeng , Yan Yingwen , Shang Shoutang , Ma Hongyu
Published: July 2023

Combustion instability is a common thermoacoustic coupling problem in combustion systems, and the pressure oscillations generated inevitably damage the combustion system. Studying the mechanism of combustion instability, especially the triggering problem of combustion instability, is particularly important for understanding combustion instability. This article adopts experimental research methods. The flame transfer function and flame describing function governing pressure pulsation were hereby measured to study the effect of heat release rate fluctuation on acoustic disturbance. By triggering combustion instability through ignition, the growth process of combustion instability was also studied. The results showed that flame pulsation amplitude shows a complex curvature when the frequency is lower than 200 Hz, while the growth rate of pulsation amplitude monotonically decreases as frequencies increase above 200 Hz. According to the considerable self−excited combustion instability tests, the oscillation amplitudes in the limit cycle state are generally greater than 0.4, while the pressure amplitudes in the limited state are less than 0.2, thus verifying the concept of a trigger threshold for low−frequency oscillation. In addition, analysis of the growth rate, the pressure and the attractor of the heat release pulsation observed after the triggering of combustion instability reveals that the triggering of combustion instability is a gradual coupling process between oscillation pressure and heat release rate pulsation.
Using Aquatic Mesocosms to Assess the Effects of Soil and Vegetation for Informing Environmental Research
Authors: Davies Jim , Melnichuk Ryan , Aumann Craig , Chen Zhongzhi , Eaton Brian
Published: July 2023

An aquatic mesocosm facility consisting of thirty 15,000 L tanks was constructed in Vegreville, Alberta to support environmental research. In 2017, an experiment was conducted as an inaugural run for the facility; this study continued through the winter of 2017/18 (over-wintering is a unique capability of the facility) and concluded in the fall of 2018. Here, we report key methods used to evaluate the effects of two independent variables: (1) a soil layer covering the floor of the mesocosms, and (2) vegetation installed in the mesocosms. Although a range of response variables were measured during this study, we limit our analysis here to the physicochemical (e.g., pH, turbidity, conductivity, and dissolved oxygen) and biological/ecological response variables (e.g., macrophytes, phytoplankton/metaphyton, and macroinvertebrates) that differed due to these two variables. The presence of a soil layer covering the floor of the mesocosm was associated with increased turbidity on some days and depths in 2017. Specific conductivity was higher in the presence of soil and its associated adventitious vegetation. During this initial study, we gained a better understanding of the characteristics and mechanics of the mesocosms, which informs design and implementation of future experiments.
Scots Pine (Pinus sylvestris L.) Ecotypes Response to Accumulation of Heavy Metals during Reforestation on Chalk Outcrops
Authors: Kosolapov Vladimir M. , Cherniavskih Vladmir I. , Dumacheva Elena V. , Sajfutdinova Luiza D. , Zavalin Alexey A. , Glinushkin Alexey P. , Kosolapova Valentina G. , Kartabaeva Bakhyt B. , Zamulina Inna V. , Kalinitchenko Valery P. , Baryshev Michail G. , Sevostyanov Michail A. , Sviridova Larisa L. , Chaplygin Victor A. , Perelomov Leonid V. , Mandzhieva Saglara S. , Burachevskaya Marina V. , Valiullin Lenar R.
Published: July 2023

As objects for reforestation, the least studied are carbonate substrates, which have a number of specific features in terms of mineral composition, the exchange of nutrients, and biological activity. The use of biological preparations of a consortium of bacteria of the genus Bacillus and mycorrhizal fungi of the genus Glomus in growing seedlings of Scots pine (Pinus sylvestris L.) on carbonate substrates provides the metabolic products; soluble and microelement salts function as catalysts for chemical reactions of exudates and soil products; and a greater amount of plant heavy metals (HM) Cu, Zn, Cd, and Pb accumulate in the soil. Among HMs, the random factors most strongly determined an accumulation of Cd (the influence rate of random factors h2x = 34.6%) and Pb (the influence rate of random factors h2x = 21.7%) in the plants. A trend of all studied HMs higher uptake by the Cretaceous pine (Pinus sylvestris var. cretacea (Kalen.) Kom.) in comparison with the P. sylvestris ecotype is revealed. Against the biological preparation background of Biogor KM and MycoCrop®, a greater value of the HM’s biological absorption in comparison with the option without biological preparations is noted. This process occurs against a background of a significant increase in the nitrification capacity in the chalk fine-grained substrate (soil aggregates < 1 mm in size), which is an indirect indicator of an increased intensity of microbiological processes. Spearman’s correlation was noted between the coefficient of accumulation of Cu, Zn, Cd, and Pb in the dry matter of Scots pine (P. sylvestris) seedlings and the nitrification capacity of substrate (rs = 0.610–0.744, p < 0.05), as well as the relationship between the nitrification capacity index of substrate and the coefficient of biological absorption of copper, zinc, and cadmium (rs = 0.543–0.765, p < 0.05). No relationship was found between the coefficient of biological absorption of lead and other soil chemical property indicators. HM absorption by plants was random. No correlations have been established between an accumulation of HMs and a content of total nitrogen, an absolute value of nitrate nitrogen, a humus content, or a pH. The significance of the work is the possibility of providing reliable reforestation with Scots pine (P. sylvestris) and Cretaceous pine (P. sylvestris var. cretacea) on the chalk outcrops using the biological preparations Biogor KM, MycoCrop®, and BGT* methodology and ensuring soil phytoremediation from HMs.
Precipitation Variations in China’s Altay Mountains Detected from Tree Rings Dating Back to AD 1615
Authors: Pang Wenxuan , Li Qiang , Liu Yu , Song Huiming , Sun Changfeng , Wang Jiachuan , Yan Yalan , Cai Qiufang , Ren Meng
Published: July 2023

As the primary mountain range in Central Asia, the Altay Mountains receive water vapor carried by westerly circulation, resulting in relatively abundant local precipitation and lush pastures in all seasons. Consequently, it has become one of the important transportation routes between Asia and Europe. The exploration of long-term variations in precipitation is meaningful for understanding the ebb and flow of the Asia–Europe steppe trade routes. However, previous dendroclimatological studies of the Altay Mountains focused more on temperature changes than precipitations variations. We carried out a 404-year precipitation reconstruction based on the tree rings of Siberian larch growing on the south slopes of the Altay Mountains, which could explain 45.9% of the variance observed in the February–October precipitation. Our reconstruction demonstrated some severe drought events which could be found in the historical documents, such as the drought in the late Ming Dynasty (1640s) and the Ding-Wu Disaster (1870s). The spatial correlation analysis, cross-wavelet spectrum and wavelet coherency analysis indicated that the precipitation variations in the study area may be related to the ENSO and NAO. This study presents a robust precipitation reconstruction of the southern Altay Mountains, serving as a reference for future research on large-scale climatic forces acting on Altay precipitation.
Characterization of Soil Microbial Biomass Carbon and Nitrogen in Four Forest Types of Shushan Urban Forest Park
Authors: Wang Mimi , Cui Jun , Liu Haiyang , Xu Xiaoniu
Published: July 2023

This study aimed to investigate the role of plantation forests and natural secondary forests in controlling soil physicochemical properties and microbial biomass in urban forest ecosystems. (1) Background: Urban forests provide numerous benefits to urban ecosystems, but the interaction between forest stands and soil properties in controlling soil microbial biomass carbon (MBC) and nitrogen (MBN) remains poorly understood. The objective of this study was to examine how different forest types (plantation forests and natural secondary forests) influence soil physicochemical properties and microbial biomass in urban forest ecosystems. (2) Methods: We conducted a study in Shushan Urban Forest Park, Hefei, China, utilizing redundancy analysis and linear regression analyses to identify key environmental factors affecting the microbial distribution and significant correlations between soil properties and microbial biomass. (3) Results: Plantation forests generally had lower pH, water content, and organic carbon and nutrient content than natural forests. Natural forests exhibited higher microbial biomass and nutrient cycling capacity. Soil depth and forest type have significant effects on soil properties and microbial biomass in both growing and dormant seasons, with practical implications for forest management and soil conservation in similar ecosystems. Soil water content (SWC), pH, total nitrogen (TN), total phosphorus (TP), and soil organic carbon (SOC) were identified as key factors affecting microbial carbon and nitrogen distribution during both growing and dormant seasons. Our study provides important insights into the role of forest stands and soil physicochemical properties in controlling soil microbial biomass in urban forest ecosystems. Effective forest management strategies should be developed to promote sustainable and resilient forest ecosystems. Future research should investigate the underlying mechanisms driving these relationships and focus on promoting sustainable and resilient urban forest ecosystems.
Wildfire Detection via a Dual-Channel CNN with Multi-Level Feature Fusion
Authors: Zhang Zhiwei , Guo Yingqing , Chen Gang , Xu Zhaodong
Published: July 2023

Forest fires have devastating impacts on ecology, the economy, and human life. Therefore, the timely detection and extinguishing of fires are crucial to minimizing the losses caused by these disasters. A novel dual-channel CNN for forest fires is proposed in this paper based on multiple feature enhancement techniques. First, the features’ semantic information and richness are enhanced by repeatedly fusing deep and shallow features extracted from the basic network model and integrating the results of multiple types of pooling layers. Second, an attention mechanism, the convolutional block attention module, is used to focus on the key details of the fused features, making the network more efficient. Finally, two improved single-channel networks are merged to obtain a better-performing dual-channel network. In addition, transfer learning is used to address overfitting and reduce time costs. The experimental results show that the accuracy of the proposed model for fire recognition is 98.90%, with a better performance. The findings from this study can be applied to the early detection of forest fires, assisting forest ecosystem managers in developing timely and scientifically informed defense strategies to minimize the damage caused by fires.
Evaluation of the Seasonal Thermal Environmental Benefits of Urban Green Space in the Core Areas of Urban Heat Island
Authors: Liu Jiachen , Wu Jianting , Yang Yong , Zhang Baolei , Yin Le
Published: July 2023

The core areas of the urban heat island (CAUHI) are the most concentrated and closely associated with humans, and they are key to managing the urban heat island (UHI). It is widely acknowledged that one of the best ways to reduce the risk of UHI is the creation of urban green spaces (UGSs). However, most of the current studies are based on the grid or block scale to explore the impact of UGS on UHI. The key to mitigating the urban heat environment is to plan urban UGS rationally in the CAUHI and explore the thermal environmental benefits of UGS. This paper provides an assessment model for the thermal environmental advantages of UGS and uses ten UGS metrics as explanatory factors for seasonal land surface temperature (LST). It quantitatively evaluates the potential differences in landscape characteristics between LST and UGS under different seasons, as well as the seasonal impact on CAUHI. This study found the following: (1) The overall distribution pattern of CAUHI shows a characteristic of spreading from the central part to the surrounding area. Most of the extremely significant CAUHI is dispersed in the center and southeastern regions of the city, where there is a much greater density of impermeable surfaces and essentially no distribution of CAUHI on the natural surface represented by forest land and water bodies. (2) Except for the aggregation index (AI), correlation analysis revealed that other metrics were highly connected with LST. Among the metrics used in this study, the largest patch index (LPI) and landscape division index (DIVISION) had the highest significant correlation with LST. Patch density (PD) was strongly negatively correlated with LST, indicating that fragmented and complex UGS patches could promote vegetation cooling. (3) The green environmental benefit index (GEBI) results showed a significant degree of spatial and temporal variability in the extracted CAUHI. This study found higher GEBI values in the larger thermal patches and lower GEBI in the surrounding smaller patches. The highest mean GEBI was found in winter, at 0.6083, and the largest distribution of large high-value patches. This study revealed the geographical and temporal variability of UGS and CAUHI, and with the help of the constructed scientific evaluation model, it offered suggestions for the optimization of urban greenery.
Thermal Comfort Analysis and Optimization Strategies of Green Spaces in Chinese Traditional Settlements
Authors: Cheng Yanyan , Bao Ying , Liu Shengshuai , Liu Xiao , Li Bin , Zhang Yuqing , Pei Yue , Zeng Zhi , Wang Zhaoyu
Published: July 2023

The spatial pattern of Weizi settlements features distinct regional characteristics. Moreover, it contains profound wisdom in terms of traditional construction; therefore, studies on its association with the microclimate have important implications for improving the quality of human settlements. In the present study, Guanweizi Village in the Xinyang City of Henan Province was used as an example to analyze and evaluate the thermal comfort of green spaces. The impact of peripheral water bodies on the thermal comfort of outdoor green spaces in the settlement was studied, and the association between the components of outdoor green spaces and physiological equivalent temperature as an indicator of thermal comfort was explored. Further, factors negatively affecting the thermal comfort of green spaces were analyzed through the grid method. Thermal comfort in the Weizi settlement is somewhat correlated with the coverage of water bodies, roads, soil, greening, and buildings. Increasing the water area and creating multi-level greening spaces are effective measures to improve the thermal comfort of green spaces in the settlement. Our findings provide a theoretical basis and a pioneering example for future practices of environment design for human settlements.
Functional Characterization of a New Salt Stress Response Gene, PeCBL4, in Populus euphratica Oliv
Authors: Qu Meiqiao , Sun Qi , Chen Ningning , Chen Zhuoyan , Zhang Hechen , Lv Fuling , An Yi
Published: July 2023

Populus euphratica is a typical stress-resistant tree species that provides valuable natural genetic resources for breeding salt-tolerant plants. The calcineurin B-like (CBL)-interacting protein kinase (CIPK) network plays an important role in regulating plant responses to abiotic stresses. The aim of this study was to characterize the function of a new CBL member, PeCBL4, in response to abiotic stresses. PeCBL4 was cloned, and sequence analysis was performed. The subcellular localization of PeCBL4 was determined using the fusion expression vector of GFP. Yeast two-hybrid assays and bimolecular fluorescence complementation were performed to identify PeCIPK members that interacted with PeCBL4. PeCBL4 was then transformed into the corresponding Arabidopsis thaliana mutants. Na+ and K+ content as well as their net fluxes were determined under high salt stress and low K+ stress. Phylogenetic tree analysis showed that PeCBL4 was clustered together with PtCBL4 and belonged to the same subgroup as AtCBL4. Subcellular localization indicated that PeCBL4 was expressed on the plasma membrane. Yeast two-hybrid assays and bimolecular fluorescence complementation showed that PeCBL4 interacted with PeCIPK24 and PeCIPK26. In addition, under high salt stress, the Na+ efflux capacities of seedlings decreased in sos3 mutants, and transgenic plants of PeCBL4 enhanced efflux capacities. In addition, the overexpression of PeCBL4 negatively influenced the influx capacity of K+. PeCBL4 interacts with PeCIPK24 and PeCIPK26 and regulates Na+/K+ balance under low K+ and high salt stress.
Maturation Stress and Wood Properties of Poplar (Populus × euramericana cv. ‘Zhonglin46’) Tension Wood
Authors: Liu Yamei , Wu Xiao , Zhang Jingliang , Liu Shengquan , Semple Katherine , Dai Chunping
Published: July 2023

Understanding the maturation stress and wood properties of poplar tension wood is critical for improving lumber yields and utilization ratio. In this study, the released longitudinal maturation strains (RLMS), anatomical features, physical and mechanical properties, and nano-mechanical properties of the cell wall were analyzed at different peripheral positions and heights in nine artificially inclined, 12-year-old poplar (Populus × euramericana cv. ‘Zhonglin46’) trees. The correlations between the RLMS and the wood properties were determined. The results showed that there were mixed effects of inclination on wood quality and properties. The upper sides of inclined stems had higher RLMS, proportion of G-layer, bending modulus of elasticity, and indentation modulus of the cell wall but a lower microfibril angle than the lower sides. At heights between 0.7 m and 2.2 m, only the double-wall thickness increased with height; the RLMS and other wood properties such as fiber length and basic density fluctuated or changed little with height. The RLMS were good indicators of wood properties in the tension wood area and at heights between 0.7 m and 1.5 m. The results of this study present opportunities to better understand the interactions and effects of these two phenomena, which both occur quite frequently in poplar stands and can influence the wood quality of valuable assortments.
A Forest Fire Susceptibility Modeling Approach Based on Integration Machine Learning Algorithm
Authors: Shi Changjiang , Zhang Fuquan
Published: July 2023

The subjective and empirical setting of hyperparameters in the random forest (RF) model may lead to decreased model performance. To address this, our study applies the particle swarm optimization (PSO) algorithm to select the optimal parameters of the RF model, with the goal of enhancing model performance. We employ the optimized ensemble model (PSO-RF) to create a fire risk map for Jiushan National Forest Park in Anhui Province, China, thereby filling the research gap in this region’s forest fire studies. Based on collinearity tests and previous research results, we selected eight fire driving factors, including topography, climate, human activities, and vegetation for modeling. Additionally, we compare the logistic regression (LR), support vector machine (SVM), and RF models. Lastly, we select the optimal model to evaluate feature importance and generate the fire risk map. Model evaluation results demonstrate that the PSO-RF model performs best (AUC = 0.908), followed by RF (0.877), SVM (0.876), and LR (0.846). In the fire risk map created by the PSO-RF model, 70.73% of the area belongs to the normal management zone, while 15.23% is classified as a fire alert zone. The feature importance analysis of the PSO-RF model reveals that the NDVI is the key fire driving factor in this study area. Through utilizing the PSO algorithm to optimize the RF model, we have addressed the subjective and empirical problems of the RF model hyperparameter setting, thereby enhancing the model’s accuracy and generalization ability.
Examining the Impact of Substrate Composition on the Biochemical Properties and Antioxidant Activity of Pleurotus and Agaricus Mushrooms
Authors: Diamantopoulou Panagiota , Fourtaka Katerina , Melanouri Eirini Maria , Dedousi Marianna , Diamantis Ilias , Gardeli Chrysavgi , Papanikolaou Seraphim
Published: July 2023

The composition of the substrate is one of the most critical factors influencing the quality as well as the nutritional value and bioactive content of mushrooms. Therefore, the effects of various substrates, such as barley and oat straw (BOS), beech wood shavings (BWS), coffee residue (CR), rice bark (RB) and wheat straw (WS, control substrate), on the biochemical properties (lipid, protein, polysaccharide, glucan, ash, and mineral content, fatty acids and tocopherols composition), total phenolic compounds and antioxidant activity of Pleurotus mushrooms, P. ostreatus (strains AMRL 144, 150) and P. eryngii (strains AMRL 166, 173-6), cultivated in ‘bag-logs’, was examined. Proximate analysis of A. bisporus and A. subrufescens grown on two different composts (C/N ratios of 10 and 13) was conducted, too. The whole carposomes, pilei and stipes were analyzed. Results showed that BOS, RB, BWS and CR improved the antioxidant activity of Pleurotus species and their nutritional characteristics. Both pilei and stipes were rich in polysaccharides (27.51–67.37 and 22.46–39.08%, w/w, for Pleurotus and Agaricus spp., respectively), lipids (0.74–8.70 and 5.80–9.92%, w/w), proteins (6.52–37.04 and 25.40–44.26, w/w, for Pleurotus and Agaricus spp., respectively) and total phenolic compounds (10.41–70.67 and 7.85–16.89 mg gallic acid equivalent/g for Pleurotus and Agaricus spp., respectively), while they contained important quantities of unsaturated FAs of nutritional and medicinal importance. Pilei were richer in proteins, total phenolic compounds and enhanced antioxidant activity and reducing power than stipes, whereas stipes were richer in IPSs and glucans compared to the corresponding pilei. Thus, mushroom cultivation could upgrade rejected agro-industrial residues and wastes to new uses as substrates for the production of mushrooms with specific nutritional and medicinal attributes.
Interaction and Metabolic Function of Microbiota during Tibetan Tea Fermentation through Bioaugmentation with Aspergillus niger
Authors: Liu Kunyi , Han Liyan , Wang Qi , Yang Liran , Liu Xiangyu , Jiang Bin , Zeng Xu , Liu Yun , Li Mingyong , Jiao Wenwen , Liu Mingli
Published: July 2023

Developing an effective method to achieve stability and improve the quality of Tibetan tea has scientific significance. Aspergillus niger K1 isolated and identified from Tibetan tea was inoculated in unsterilized or sterilized tea leaves to develop the bioaugmented fermentation (BF) and normal fermentation (NF) processes of Tibetan tea. The results showed that BF resulted in infusions with a deeper color, a stronger aroma, and a thicker taste compared to NF. The dominant bacterium in BF was Staphylococcus (23.76%), while the dominant fungus was Blastobotrys adeninivorans (50.95%). Moreover, 859 metabolites were identified, and the level of 90 differentially changed metabolites (DCMs) in BF increased significantly (VIP > 1, p < 0.05, FC > 2) compared to those in NF, while the level of 37 DCMs in BF decreased significantly (VIP > 1, p < 0.05, FC < 0.5). Correlation analysis demonstrated that A. niger significantly positively correlated with theabrownins, caffeine, and glutamylisoleucine (p < 0.05, |r| > 0.8). B. adeninivorans showed significant negative correlations with 1-(beta-D-ribofuranosyl)-1,4-dihydronicotinamide and 2-hydroxyacetaminophen sulfate (p < 0.05, |r| > 0.8). Consequently, the inoculation of A. niger for BF has the potential to alter the metabolites in tea through a synergistic interaction with other microorganisms, ultimately improving the sensory quality of Tibetan tea.
Molecular Characterization and Expression Analysis of NLRC3-like, ASC, and Caspase1 in Spotted Sea Bass (Lateolabrax maculatus)
Authors: Yuan Shuya , Sun Zhaosheng , Gao Qian , Li Zhen , Qi Zhitao , Zheng Sidi , Liu Danjie
Published: July 2023

Nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) family members are innate immune sensors involved in the recognition of highly conserved pathogen-associated molecular patterns (PAMPs). Apoptosis-associated speck-like protein (ASC) is a critical adaptor molecule in multiple inflammasome protein complexes, mediating inflammation and host defense. Caspase1, an inflammatory caspase, has been documented to play important roles in the innate immune system. In this study, we identified and characterized NLRC3-like, ASC, and Caspase1 (referred to as LmNLRC3L, LmASC, and LmCaspase1) from the spotted sea bass (Lateolabrax maculatus). A sequence analysis revealed that LmNLRC3L, LmASC, and LmCaspase1 shared similar features with their fish counterparts. LmNLRC3L contained a FISNA domain, a NACHT domain, and four LRR motifs, followed by a C-terminal fish-specific B30.2 domain. LmASC possessed a PYRIN domain for interacting with inflammasome sensor proteins, as well as a CARD domain. LmCaspase1 had a CARD domain at its N-terminus and a CASC domain at its C-terminus. These three genes were ubiquitously distributed in the liver, spleen, head kidney, gill, intestine, skin, muscle, and brain. They share similar expression patterns, and all demonstrate the highest level of expression in the gill. We analyzed the expression changes in genes in the spleen, gill, and head kidney after stimulation experiments in vivo. After lipopolysaccharide (LPS) stimulation, the expression levels of these three genes were significantly upregulated in the short term, followed by significant downregulation at 48 and 72 h in some examined tissues. Following Edwardsiella tarda infection, these three genes were upregulated in various tissues. However, the expressions of these three genes were not affected by polyinosinic-polycytidylic acid (poly (I:C)) stimulation. Overall, our results indicate that these three genes are involved in the immune response against bacterial infection in the spotted sea bass, providing the foothold for understanding the immune function and mechanism of the fish inflammasome.
Analysis of Volatile Markers and Their Biotransformation in Raw Chicken during Staphylococcus aureus Early Contamination
Authors: Wang Yin , Wang Xian , Huang Yuanyuan , Yue Tianli , Cao Wei
Published: July 2023

To address the potential risks to food safety, headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS) and headspace gas chromatography–ion mobility spectrometry (HS-GC-IMS) were used to analyze the volatile organic compounds (VOCs) generated from chilled chicken contaminated with Staphylococcus aureus during early storage. Together with the KEGG database, we analyzed differential metabolites and their possible biotransformation pathways. Orthogonal partial least squares discriminant analysis (OPLS-DA) was applied to characterize VOCs and identify biomarkers associated with the early stage of chicken meat contamination with S. aureus. The results showed 2,6,10,15-tetramethylheptadecane, ethyl acetate, hexanal, 2-methylbutanal, butan-2-one, 3-hydroxy-2-butanone, 3-methylbutanal, and cyclohexanone as characteristic biomarkers, and 1-octen-3-ol, tetradecane, 2-hexanol, 3-methyl-1-butanol, and ethyl 2-methylpropanoate as potential characteristic biomarkers. This provides a theoretical basis for the study of biomarkers of Staphylococcus aureus in poultry meat.
Characterization of a Dihydromyricetin/α-Lactoalbumin Covalent Complex and Its Application in Nano-emulsions
Authors: Lu Ninghai , Wu Limin , Zhen Shiyu , Liu Benguo
Published: July 2023

A dihydromyricetin (DMY)/α-lactoalbumin (α-La) covalent complex was prepared and characterized, and its application in nano-emulsions was also evaluated in this study. The results suggested that the covalent complex could be obtained using the alkaline method. The UV and IR spectra confirmed the formation of the covalent complex, and the amount of DMY added was positively correlated with the total phenol content of the complex. The complex had an outstanding 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)-radical-scavenging ability, reducing power and α-glucosidase inhibitory activity, which were positively related to its total phenol content. The complex could be used as an emulsifier to stabilize the β-carotene-loaded nano-emulsion. The stability and β-carotene-protective capacity of the nano-emulsion stabilized by the complex were also positively related to the total phenol content of the complex, being higher than those of the nano-emulsion developed using α-La. Our results provide a reference for the construction of a new food delivery system and extend the applications of α-La and DMY in foods.
The Potential of Soluble Gas Stabilization (SGS) Technology in a Simulated Post-Frying Cooling Step of Commercial Fish Cakes
Authors: Rotabakk Bjørn Tore , Rognstad Elena Marie , Jakobsen Anita Nordeng , Lerfall Jørgen
Published: July 2023

Soluble gas stabilization (SGS) technology is a novel way to increase the effectiveness of modified atmosphere (MA) packaging. However, SGS can be time-consuming and difficult to include in an existing process. This can be overcome by including CO2 in an existing processing step, such as the product’s cooling step. A full factorial design was set up with SGS times (0.5, 1.0, and 2.0 h) and temperatures of fish cakes (chilled (0 °C) or during chilling (starting at 85 °C)) as factors. MA-packaged fish cakes were included as a control. The response was headspace gas composition at equilibrium. Headspace gas composition at equilibrium showed significantly (p < 0.05) less dissolved CO2 in hot fish cakes after 0.5 h than in cold cakes. Still, no significant differences were found between hot and cold at 1.0 and 2.0 h. Also, all SGS samples, regardless of time and temperature, had a higher content of CO2 compared to modified atmosphere packaging (MAP).
Recent Advances in Electrochemical Biosensors for the Detection of Foodborne Pathogens: Current Perspective and Challenges
Authors: Wang Bo , Wang Hang , Lu Xubin , Zheng Xiangfeng , Yang Zhenquan
Published: July 2023

Foodborne pathogens cause many diseases and significantly impact human health and the economy. Foodborne pathogens mainly include Salmonella spp., Escherichia coli, Staphylococcus aureus, Shigella spp., Campylobacter spp. and Listeria monocytogenes, which are present in agricultural products, dairy products, animal-derived foods and the environment. Various pathogens in many different types of food and water can cause potentially life-threatening diseases and develop resistance to various types of antibiotics. The harm of foodborne pathogens is increasing, necessitating effective and efficient methods for early monitoring and detection. Traditional methods, such as real-time polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA) and culture plate, are time-consuming, labour-intensive and expensive and cannot satisfy the demands of rapid food testing. Therefore, new fast detection methods are urgently needed. Electrochemical biosensors provide consumer-friendly methods to quickly detect foodborne pathogens in food and the environment and achieve extensive accuracy and reproducible results. In this paper, by focusing on various mechanisms of electrochemical transducers, we present a comprehensive overview of electrochemical biosensors for the detection of foodborne pathogens. Furthermore, the review introduces the hazards of foodborne pathogens, risk analysis methods and measures of control. Finally, the review also emphasizes the recent research progress and solutions regarding the use of electrochemical biosensors to detect foodborne pathogens in food and the environment, evaluates limitations and challenges experienced during the development of biosensors to detect foodborne pathogens and discusses future possibilities.
Recent Progress in Hyaluronic-Acid-Based Hydrogels for Bone Tissue Engineering
Authors: Hwang Hee Sook , Lee Chung-Sung
Published: July 2023

Hydrogel-based bone tissue engineering is a potential strategy for treating bone abnormalities and fractures. Hyaluronic acid (HA) is a natural polymer that is widely distributed in the human body and plays a significant role in numerous physiological processes such as cell migration, tissue hydration, and wound healing. Hydrogels based on HA and its derivatives have gained popularity as potential treatments for bone-related diseases. HA-based hydrogels have been extensively studied for their ability to mimic the natural extracellular matrix of bone tissue and provide a suitable microenvironment for cell support and tissue regeneration. The physical and chemical properties of HA can be modified to improve its mechanical strength, biocompatibility, and osteogenic potential. Moreover, HA-based hydrogels combined with other biomaterials in the presence or absence of bioactive agents have been investigated as a means of improving the mechanical properties and bioactivity of the hydrogel scaffold. Therefore, HA-based hydrogels have shown great promise in bone tissue engineering due to their biocompatibility, osteogenic activity, and ability to mimic the natural extracellular matrix of bone tissue. Overall, this review provides a comprehensive overview of the current state of the art in HA-based hydrogels for bone tissue engineering, highlighting the key advances, challenges, and future directions in this rapidly evolving field.
The Diversity in Grapes of Vitis labrusca Grown in Bolu (Türkiye) Assessed by Multivariate Approaches
Authors: Güler Emrah , Kan Emre , Ünal Mehmet
Published: July 2023

The grape is one of the most produced and processed horticultural crops. This study evaluated the grape genetic resource belonging to the Vitis labrusca species. The diversity was assessed according to morphometric, antioxidant, physicochemical, and colorimetric characteristics. The diversity was evaluated using a variation index and multivariate analyses. The bunch weight of the vines exhibited a range from 21.05 g to 162.46 g, with a coefficient of variation (CV) of 38.97%. The average bunch weight was 64.74 g. In terms of the berry properties, the highest CV was observed for the berry weight (21.95%). The peel thickness displayed a CV of 36.40%, and an average of 0.23 mm. The CVs for the juice characteristics in the berries of the studied vines were 7.11%, 16.61%, 19.41%, and 28.10% for the pH, TSS, must yield, and TA, respectively. The TPC of the accessions exhibited a notably low variation (CV = 4.63%). The color properties of the accessions displayed an immense variation, except for the L* values. The hierarchical clustering analysis divided the accessions into two main clusters, which both had two subclusters. The multivariate approaches separated individuals into different groups, and they were considered useful tools for utilization in the genetic diversity assessments. Further studies on the cultivation technique and crossbreeding with Vitis vinifera will provide more insights into the population, and this study will be a source for upcoming studies on V. labrusca in the region.
Morphology and Mitochondrial Lineage Investigations Corroborate the Systematic Status and Pliocene Colonization of Suncus niger (Mammalia: Eulipotyphla) in the Western Ghats Biodiversity Hotspot of India
Authors: Kundu Shantanu , Kamalakannan Manokaran , Kim Ah Ran , Hegde Vishwanath D. , Banerjee Dhriti , Jung Won-Kyo , Kim Young-Mog , Kim Hyun-Woo
Published: July 2023

The Indian highland shrew, Suncus niger (Horsfield, 1851), is the least studied soricid species from its original range distribution in Southern India, with several systematics conundrums. Following its discovery in 1851, the species was synonymized with Suncus montanus (Kelaart, 1850) (endemic to Sri Lanka) and subsequently identified as a separate Indian population. However, the systematic status of S. niger from topotype specimens in Southern India has yet to be determined through an integrated approach. Both taxonomy and mitochondrial genetic data (Cytochrome b and 16S ribosomal RNA) were used to re-examine the systematics of S. niger. The mtCytb gene clearly distinguished topotypic S. niger from other Suncus species, with high genetic divergences varying from 8.49% to 26.29%. Further, the Bayesian and maximum likelihood topologies clearly segregated S. niger from other congeners and corroborated the sister relationship with S. stoliczkanus with expected divergence in the late Pliocene (2.62 MYA). The TimeTree analysis also exhibits a strong matrilineal affinity of S. dayi (endemic to India) toward the African species. The current study hypothesizes that the ancestor of the soricids evolved in Africa and that genetic lineages were subsequently shifted by plate tectonic events that subsequently colonized different continents as distinct species during the late Miocene (Tortonian) to the Holocene era. In addition to the new range expansion and elevation records of S. niger in the Central Western Ghats, we propose that additional sampling across its distribution, as well as the use of multiple genetic markers, may be useful in determining the genetic diversity and population structure of this endemic species. The present study also recommends that more molecular data on the Soricomorphs lineages, and estimates of their divergence times, will shed light on the evolution of these small mammals on Earth.
Evolutionary Rates, Divergence Rates, and Performance of Individual Mitochondrial Genes Based on Phylogenetic Analysis of Copepoda
Authors: He Junzong , Zhou Zhihao , Huang Yan , Feng Jinmei , Li Wenxiang , Wang Guitang , Hua Congjie
Published: July 2023

Copepoda is a large and diverse group of crustaceans, which is widely distributed worldwide. It encompasses roughly 9 orders, whose phylogeny remains unresolved. We sequenced the complete mitochondrial genome (mitogenome) of Sinergasilus major (Markevich, 1940) and used it to explore the phylogeny and mitogenomic evolution of Copepoda. The mitogenome of S. major (14,588 bp) encodes the standard 37 genes as well as a putative control region, and molecular features are highly conserved compared to other Copepoda mitogenomes. Comparative analyses indicated that the nad2 gene has relatively high nucleotide diversity and evolutionary rate, as well as the largest amount of phylogenetic information. These results indicate that nad2 may be a better marker to investigate phylogenetic relationships among closely related species in Copepoda than the commonly used cox1 gene. The sister-group relationship of Siphonostomatoida and Cyclopoida was recovered with strong support in our study. The only topological ambiguity was found within Cyclopoida, which might be caused by the rapid evolution and sparse taxon sampling of this lineage. More taxa and genes should be used to reconstruct the Copepoda phylogeny in the future.
Identification of ROH Islands Conserved through Generations in Pigs Belonging to the Nero Lucano Breed
Authors: Di Gregorio Paola , Perna Annamaria , Di Trana Adriana , Rando Andrea
Published: July 2023

The recovery of Nero Lucano (NL) pigs in the Basilicata region (Southern Italy) started in 2001 with the collaboration of several public authorities in order to preserve native breeds that can play a significant economic role both due to their remarkable ability to adapt to difficult environments and the value of typical products from their area of origin. In this study, by using the Illumina Porcine SNP60 BeadChip, we compared the genetic structures of NL pigs reared in a single farm in two different periods separated by a time interval corresponding to at least three generations. The results showed an increase in the percentage of polymorphic loci, a decrease in the inbreeding coefficient calculated according to ROH genome coverage (FROH), a reduction in the number of ROH longer than 16 Mb and an increase in ROH with a length between 2 and 4 Mb, highlighting a picture of improved genetic variability. In addition, ROH island analysis in the two groups allowed us to identify five conserved regions, located on chromosomes 1, 4, 8, 14 and 15, containing genes involved in biological processes affecting immune response, reproduction and production traits. Only the conserved ROH island on chromosome 14 contains markers which, according to the literature, are associated with QTLs affecting thoracic vertebra number, teat number, gestation length, age at puberty and mean platelet volume.
Effects of an Orff Music Activity Intervention Program on the Ego-Resilience, Peer Relationships, Happiness, Interpersonal Care Awareness, Anxiety, and Stress of Children from Multicultural Families in Republic of Korea
Authors: Kim Su-Hee , Lee Sook
Published: July 2023

This study developed an interpersonal care-based Orff music activity program for children from multicultural families in Republic of Korea. We measured its effects on ego-resilience, peer relationships, happiness, interpersonal care awareness, anxiety, and stress. The participants were 74 children from third to sixth grade attending 10 regional children’s centers in Cheonan-si, Chungcheongnam-do Province, Republic of Korea. They were randomly assigned to 36 experimental groups and 38 control groups. The experimental program was developed based on investigations of human care theory, expressions of care, and Orff music activities. The experiment was conducted twice a week for five weeks in 45 min sessions. Data were collected from June to September 2020. We used questionnaires on ego-resilience, peer relationships, happiness, and interpersonal care awareness to measure the effects, which were analyzed through frequency and descriptive statistics. Anxiety, physiological anxiety, acculturation stress, and physiological stress were measured using Behavior Detection VibraImage System Version 8.1 PRO (VIBRASYSTEM Ltd., Co., Seoul, Republic of Korea). The homogeneity of the variables across groups was examined using the χ² test, t-test, and Fisher’s exact test. The effects were examined using repeated measures variance analysis and independent t-tests. The results showed that the program had significant effects on ego-resilience, peer relationships, happiness, interpersonal care awareness, physiological anxiety, and physiological stress. The findings suggest that interpersonal care nursing can be effective for children from multicultural families, and the program can be used for intervention to improve children’s mental health.
Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants
Authors: Fernandes Ângela , Polyzos Nikolaos , Mandim Filipa , Pereira Carla , Petrović Jovana , Soković Marina , Petropoulos Spyridon A.
Published: July 2023

The aim of the present study was to evaluate the possible effects of two different biostimulant formulations at different application regimes and combined or not with mineral fertilizers (e.g., W1–W8, including the control treatment (no formulations added)) on the yield parameters and fruit quality of watermelon plants. The highest yield was recorded for the W5 treatment due to the formation of more fruit. The highest content of fat, proteins and ash was recorded for treatment W1, whereas carbohydrates were the most abundant in the control treatment, resulting also in the highest energetic value. The main detected sugars in all the tested samples were sucrose and fructose, which were the highest for the W4 and W5 treatments (sucrose) and W4 treatment (fructose). Malic and citric acid were the most abundant compounds, especially in the W4 treatment. In terms of tocopherols, only α-tocopherol was detected, with the highest amounts being recorded for the W4 treatment. Regarding bioactive properties, the lowest IC50 values for OxHLIA were recorded for the W2, W3 and W8 formulations. Moreover, all the extracts exhibited significant anti-inflammatory activity comparable to the positive control, while a variable efficacy of the tested extracts against the studied bacteria and fungi was recorded. In conclusion, our results indicate that simple agronomic practices such as biostimulant application may improve crop performance and improve the proximal composition and the overall quality of watermelon fruit within the context of sustainable crop production.