bluuebunny/crossref_metadata_2025 huggingface dataset:
Ameliorative Hematological and Histomorphological Effects of Dietary Trigonella foenum-graecum Seeds in Common Carp (Cyprinus carpio) Exposed to Copper Oxide Nanoparticles
Authors: Noureen Aasma , De Marco Giuseppe , Rehman Nagina , Jabeen Farhat , Cappello Tiziana
Published: October 2022

Different types of metal oxide nanoparticles (NPs) are being used for wastewater treatment worldwide but concerns have been raised regarding their potential toxicities, especially toward non-targeted aquatic organisms including fishes. Therefore, the present study aimed to evaluate the toxicity of copper oxide (CuO) NPs (1.5 mg/L; positive control group) in a total of 130 common carp (Cyprinus carpio), as well as the potential ameliorative effects of fenugreek (Trigonella foenum-graecum) seed extracts (100 mg/L as G-1 group, 125 mg/L as G-2 group, and 150 mg/L as G-3 group) administered to fish for 28 days. Significant changes were observed in the morphometric parameters: the body weight and length of the CuO-NP-treated fish respectively decreased from 45.28 ± 0.34 g and 14.40 ± 0.56 cm at day one to 43.75 ± 0.41 g and 13.57 ± 0.67 cm at day 28. Conversely, fish treated with T. foenum-graecum seed extract showed significant improvements in body weight and length. After exposure to CuO NPs, a significant accumulation of Cu was recorded in the gills, livers, and kidneys (1.18 ± 0.006 µg/kg ww, 1.38 ± 0.006 µg/kg ww, and 0.05 ± 0.006 µg/kg ww, respectively) of the exposed common carp, and significant alterations in fish hematological parameters and oxidative stress biomarkers (lipid peroxidation (LPO), glutathione (GSH), and catalase (CAT)) were also observed. However, supplementing diets with fenugreek extracts modulated the blood parameters and the oxidative stress enzymes. Similarly, histological observations revealed that sub-lethal exposure to CuO NPs caused severe histomorphological changes in fish gills (i.e., degenerative epithelium, fused lamellae, necrotic lamellae, necrosis of primary lamellae, complete degeneration, and complete lamellar fusion), liver (i.e., degenerative hepatocytes, vacuolization, damaged central vein, dilated sinusoid, vacuolated degeneration, and complete degeneration), and kidney (i.e., necrosis and tubular degeneration, abnormal glomerulus, swollen tubules, and complete degeneration), while the treatment with the fenugreek extract significantly decreased tissue damage in a dose-dependent manner by lowering the accumulation of Cu in the selected fish tissues. Overall, this work demonstrated the ameliorative effects of dietary supplementation with T. foenum-graecum seed extract against the toxicity of NPs in aquatic organisms. The findings of this study therefore provided evidence of the promising nutraceutical value of fenugreek and enhanced its applicative potential in the sector of fish aquaculture, as it was shown to improve the growth performance and wellness of organisms.
Status and Influencing Factors of Physical Exercise among College Students in China: A Systematic Review
Authors: Pan Mingzhu , Ying Binbin , Lai Yani , Kuan Garry
Published: October 2022

The status of the physical exercise of college students has been a popular topic in China. This study systematically reviewed the exercise status of Chinese college students and its influencing factors. A keyword and reference search were conducted in the Web of Science, PubMed, Cochrane library, and the China National Knowledge Infrastructure. Additionally, Google Scholar was searched to collect literatures related to physical activity of Chinese university students published in Chinese and English from 1 January 2017 to 30 July 2022. Fifteen studies met the selection criteria and were included in the review. The results show that the main motivation for Chinese college students to exercise is to strengthen their bodies, with running and walking ranking first and ball games ranking second in importance. Most of the college students exercised three times a week, which is the recommended minimum, and most of their workouts were of moderate intensity. Additionally, the workouts lasted for 30 min to 60 min. The main factors affecting college students’ exercise are lack of time due to academic pressure, facilities constraints, and lack of professional exercise guidance. In conclusion, the physical fitness of university students should not be underestimated, and this study provides additional reference to promote healthier lifestyles among Chinese college students.
An Assessment of Mortality among Elderly Brazilians from Alcohol Abuse Diseases: A Longitudinal Study from 1996 to 2019
Authors: Barbosa João de Sousa Pinheiro , Pereira Leonardo Costa , Salgado Fabiana Xavier Cartaxo , da Silva Rodrigo Marques , Karnikowski Margô Gomes de Oliveira
Published: October 2022

Alcohol use disorder (AUD) is a worldwide public health problem, being an important aggravating factor of comorbidities found in the elderly, with the potential to increase mortality indicators for this age group. Objective: To analyze alcohol-induced deaths in elderly people with alcohol-related disorder in Brazil between 1996 and 2019. Methods: An ecological study was conducted with secondary data obtained from the Brazilian Unified Health System (SIM) Mortality Information System from 1996 to 2019. TabNet/DATASUS, Excel® 2016 and SPSS 21® were used to prepare the results. Results: Between 1996 and 2019, 85,928 alcohol-induced deaths were recorded among the elderly (>60 years); in 1996, the lowest number of deaths was recorded (n = 1396), and in 2018, there were the highest number of deaths (n = 5667). In the profile of the elderly, there was a predominance of men (88%). Mortality from AUD was due to alcoholic liver disease (62.2%), followed by mental disorders due to alcohol use (37.3%). Conclusions: Coping with AUD is a public health problem that aims to reduce the number of deaths from diseases, conditions and injuries in which alcohol consumption is the causative agent, in addition to preventing deaths to which alcohol contributes.
Broccoli Sprouts Promote Sex-Dependent Cardiometabolic Health and Longevity in Long-Evans Rats
Authors: Noble Ronan M. N. , Jahandideh Forough , Armstrong Edward A. , Bourque Stephane L. , Yager Jerome Y.
Published: October 2022

Antioxidants and anti-inflammatory compounds are potential candidates to prevent age-related chronic diseases. Broccoli sprouts (BrSp) are a rich source of sulforaphane—a bioactive metabolite known for its antioxidant and anti-inflammatory properties. We tested the effect of chronic BrSp feeding on age-related decline in cardiometabolic health and lifespan in rats. Male and female Long-Evans rats were fed a control diet with or without dried BrSp (300 mg/kg body weight, 3 times per week) from 4 months of age until death. Body weight, body composition, blood pressure, heart function, and glucose and insulin tolerance were measured at 10, 16, 20, and 22 months of age. Behavioral traits were also examined at 18 months of age. BrSp feeding prolonged life span in females, whereas in males the positive effects on longevity were more pronounced in a subgroup of males (last 25% of survivors). Despite having modest effects on behavior, BrSp profoundly affected cardiometabolic parameters in a sex-dependent manner. BrSp-fed females had a lower body weight and visceral adiposity while BrSp-fed males exhibited improved glucose tolerance and reduced blood pressure when compared to their control counterparts. These findings highlight the sex-dependent benefits of BrSp on improving longevity and delaying cardiometabolic decline associated with aging in rats.
Pharmacophore Modeling Using Machine Learning for Screening the Blood–Brain Barrier Permeation of Xenobiotics
Authors: Kumar Saurav , Deepika Deepika , Kumar Vikas
Published: October 2022

Daily exposure to xenobiotics affects human health, especially the nervous system, causing neurodegenerative diseases. The nervous system is protected by tight junctions present at the blood–brain barrier (BBB), but only molecules with desirable physicochemical properties can permeate it. This is why permeation is a decisive step in avoiding unwanted brain toxicity and also in developing neuronal drugs. In silico methods are being implemented as an initial step to reduce animal testing and the time complexity of the in vitro screening process. However, most in silico methods are ligand based, and consider only the physiochemical properties of ligands. However, these ligand-based methods have their own limitations and sometimes fail to predict the BBB permeation of xenobiotics. The objective of this work was to investigate the influence of the pharmacophoric features of protein–ligand interactions on BBB permeation. For these purposes, receptor-based pharmacophore and ligand-based pharmacophore fingerprints were developed using docking and Rdkit, respectively. Then, these fingerprints were trained on classical machine-learning models and compared with classical fingerprints. Among the tested footprints, the ligand-based pharmacophore fingerprint achieved slightly better (77% accuracy) performance compared to the classical fingerprint method. In contrast, receptor-based pharmacophores did not lead to much improvement compared to classical descriptors. The performance can be further improved by considering efflux proteins such as BCRP (breast cancer resistance protein), as well as P-gp (P-glycoprotein). However, the limited data availability for other proteins regarding their pharmacophoric interactions is a bottleneck to its improvement. Nonetheless, the developed models and exploratory analysis provide a path to extend the same framework for environmental chemicals, which, like drugs, are also xenobiotics. This research can help in human health risk assessment by a priori screening for neurotoxicity-causing agents.
Vaccinated and Unvaccinated Risk Perceptions and Motivations for COVID-19 Preventive Measures Based on EPPM—A Polish Qualitative Pilot Study
Authors: Domosławska-Żylińska Katarzyna , Krysińska-Pisarek Magdalena , Czabanowska Katarzyna , Sesa Giulia
Published: October 2022

COVID-19 has been a “major interrupting event” during which individuals largely relied on intuitive risk perceptions as precursors of COVID-19 health behaviors. Given the strong correlation between risk perceptions and the adoption of preventive measures, this pilot study attempts to explore how Polish society perceives COVID-19 health risks from the point of view of vaccinated and unvaccinated individuals. The Extended Parallel Process Model (EPPM) constitutes the basis for this research. Two focus group discussions (FGDs) were conducted, one with participants who had received COVID-19 vaccinations and the other with participants who had not. Conventional and directed content analyses were used to examine the data from the FGDs. Five categories emerged from the data: COVID-19 risk perceptions, coping with the pandemic, sources of knowledge, distrust, and pandemic fatigue. All categories fit into the theoretical constructs of the EPPM. Both groups have a high-perceived vulnerability to COVID-19 infection and recognize its seriousness. Individuals also have a high perceived response self-efficacy, given their awareness of COVID-19 preventive measures and how these should be applied. Nonetheless, particularly the unvaccinated, are skeptical about the effectiveness of the implemented measures, showing low perceived response efficacy. Future communication strategies should target the effectiveness of COVID-19 preventive measures, and one’s perceived response efficacy, to improve adherence to public health measures.
Changes in Prevalence and Determinants of Self-Reported Hypertension among Bangladeshi Older Adults during the COVID-19 Pandemic
Authors: Mistry Sabuj Kanti , Ali ARM Mehrab , Yadav Uday Narayan , Khanam Fouzia , Huda Md. Nazmul , Lim David , Chowdhury ABM Alauddin , Sarma Haribondhu
Published: October 2022

The present study aimed to assess the changes in the prevalence and determinants of self-reported hypertension among older adults during the COVID-19 pandemic in Bangladesh. This repeated cross-sectional study was conducted on two successive occasions (October 2020 and September 2021), overlapping the first and second waves of the COVID-19 pandemic in Bangladesh. The survey was conducted through telephone interviews among Bangladeshi older adults aged 60 years and above. The prevalence of hypertension was measured by asking a question about whether a doctor or health professional told the participants that they have hypertension or high blood pressure and/or whether they are currently using medication to control it. We also collected information on the socio-economic characteristics of the participants, their cognitive ability, and their COVID-19-related attributes. A total of 2077 older adults with a mean age of 66.7 ± 6.4 years participated in the study. The samples were randomly selected on two successive occasions from a pre-established registry developed by the ARCED Foundation. Thus, the sample in the 2021-survey (round two; n = 1045) was not the same as that in the 2020-survey (round one; n = 1031) but both were drawn from the same population. The findings revealed that the prevalence of hypertension significantly increased across the two periods (43.7% versus 56.3%; p = 0.006). The odds of hypertension were 1.34 times more likely in round two than in the round one cohort (AOR 1.34, 95% CI 1.06–1.70). We also found that having formal schooling, poorer memory or concentration, and having had received COVID-19 information were all associated with an increased risk of hypertension in both rounds (p < 0.05). The findings of the present study suggest providing immediate support to ensure proper screening, control, and treatment of hypertension among older adults in Bangladesh.
Teachers’ Professional Identity and Burnout among Chinese Female School Teachers: Mediating Roles of Work Engagement and Psychological Capital
Authors: Sun Changkang , Feng Xuechao , Sun Binghai , Li Weijian , Zhong Chenyin
Published: October 2022

Burnout is a serious phenomenon among female kindergarten, primary, and secondary school teachers in China. Previous research has shown that professional identity negatively predicts burnout. However, little is known about the mediating mechanisms underlying this relationship. This study examined the relationship between professional identity and burnout and the mediating roles of work engagement and psychology using a sample of Chinese teachers. A total of 2220 female teachers participated (kindergarten: 16.9%; primary school: 56.7%; secondary school: 26.4%). They answered four questionnaires measuring their professional identity, work engagement, psychological capital, and burnout. PROCESS macro (SPSS 21.0) was used to conduct mediation analyses of work engagement and psychological capital in the relationship between professional identity and burnout. Working simultaneously, work engagement, and psychological capital partially mediated the aforementioned relationship, which could reduce burnout. Working sequentially completely mediated the relationship between professional identity and burnout, and hence, the latter was the lowest. Specific implications are discussed, such as the improvement of professional identity and psychological capital.
An Optimized Damping Grey Population Prediction Model and Its Application on China’s Population Structure Analysis
Authors: Guo Xiaojun , Zhang Rui , Shen Houxue , Yang Yingjie
Published: October 2022

Population, resources and environment constitute an interacting and interdependent whole. Only by scientifically forecasting and accurately grasping future population trends can we use limited resources to promote the sustainable development of society. Because the population system is affected by many complex factors and the structural relations among these factors are complex, it can be regarded as a typical dynamic grey system. This paper introduces the damping accumulated operator to construct the grey population prediction model based on the nonlinear grey Bernoulli model in order to describe the evolution law of the population system more accurately. The new operator can give full play to the principle of new information first and further enhance the ability of the model to capture the dynamic changes of the original data. A whale optimization algorithm was used to optimize the model parameters and build a smooth prediction curve. Through three practical cases related to the size and structure of the Chinese population, the comparison with other grey prediction models shows that the fitting and prediction accuracy of the damping accumulated–nonlinear grey Bernoulli model is higher than that of the traditional grey prediction model. At the same time, the damping accumulated operator can weaken the randomness of the original data sequence, reduce the influence of external interference factors, and enhance the robustness of the model. This paper proves that the new method is simple and effective for population prediction, which can not only grasp the future population change trend more accurately but also further expand the application range of the grey prediction model.
Suicide Risk in Military Personnel during the COVID-19 Health Emergency in a Peruvian Region: A Cross-Sectional Study
Authors: Valladares-Garrido Mario J. , Picón-Reátegui Cinthia Karina , Zila-Velasque J. Pierre , Grados-Espinoza Pamela , Hinostroza-Zarate Cristian M. , Failoc-Rojas Virgilio E. , Pereira-Victorio César Johan
Published: October 2022

Military personnel represent a frontline group exposed to multiple stressors. These factors have increased during the COVID-19 pandemic, predisposing to the development of suicidal risk (SR). Given the few studies conducted in this population, we evaluated the prevalence of SR and its associated factors during the health emergency. A cross-sectional survey study was conducted in person among 514 participants in Lambayeque, Peru in 2021. The outcome was SR, and the exposures were depression (PHQ-9), anxiety (GAD-7), PTSD (PCL-C), and other sociodemographic variables. The prevalence of SR was 14.0% (95% CI: 11.12–17.31%) and was significantly higher in people with a family history of mental health (PR: 2.16; 95% CI: 1.13–4.15) and in those with moderate clinical insomnia (PR: 2.21; 95% CI: 1.19–4.12). Military personnel with high resilience had a lower prevalence of SR (PR: 0.54, CI: 0.31–0.95). Anxiety was associated with a higher prevalence of SR (PR: 3.27; 95% CI: 1.76–6.10). Our findings show that at least 1 out of 10 military personnel are at risk of suicide. Special attention should be paid to the associated factors to develop interventions and reverse their consequences. These results may be useful in policy implementation and general statistics of SR in the local and regional context.
Multiscale Impact of Environmental and Socio-Economic Factors on Low Physical Fitness among Chinese Adolescents and Regionalized Coping Strategies
Authors: Tong Zihan , Kong Zhenxing , Jia Xiao , Zhang Hanyue , Zhang Yimin
Published: October 2022

As low physical fitness in adolescents increases their risk of all-cause mortality in future adulthood as well as regional public health budgets, many scholars have studied the factors influencing physical fitness in adolescents. However, the spatial non-stationarity and scale between physical fitness and influencing factors in adolescents are often neglected. To rectify this situation, this study constructed a multi-scale geographically weighted regression model based on data from the China National Student Fitness Survey and the China Statistical Yearbook in 2018 to investigate the spatial patterns of factors influencing low physical fitness among adolescents. The results showed that the influencing factors for measuring the physical fitness of Chinese adolescents had significant spatial heterogeneity and multi-scale effects. The local R2 values were relatively low in the western region of China. Consideration should be given to increasing the lifestyle and ethnic and cultural characteristics of local residents when selecting influencing factors in the future. The physical fitness of men was mainly influenced by socio-economic factors, while that of women was influenced by natural environmental factors. According to the different spatial distribution patterns of MGWR, this study suggests that each region should develop regionalized strategies to cope with the low physical fitness of adolescents, including taking advantage of the natural environment to develop physical fitness promotion projects, accelerating the upgrading of industrial structures in the north-eastern and western regions, and the need to remain cautious of rapid urbanization in the east.
Aesthetics and Cartography: Post-Critical Reflections on Deviance in and of Representations
Authors: Edler Dennis , Kühne Olaf
Published: October 2022

Cartographic representations are subject to sensory perception and rely on the translation of sensory perceptions into cartographic symbols. In this respect, cartography is closely related to aesthetics, as it represents an academic discipline of sensory perceptions. The scholarly concern with cartographic aesthetics, by today, has strongly been focused on the aesthetic impact of cartographic representations. The consideration of the philosophical sub-discipline of aesthetics however is rather restrained. This is also true for the connection between sociological questions and the social construction of aesthetic judgments. We address both topics in this article. We refer to post-critical cartographic theory. It accepts the socially constructed nature and power-bound nature of maps but does not reject “traditional” and widely established positivist cartography. Drawing on the theory of deviant cartographies related to this, we understand cartography designed according to aesthetic criteria as meta-deviant, as it makes the contingency of world interpretations clear. Especially augmented and virtual environments show a great potential to generate aesthetically constructed cartographic representations. Participatory cartography enables many people to reflect on the contingency of their spatial experiences and spatial abstractions without expert-like special knowledge. A prerequisite, however, is the greatest possible openness to topics and representations. This is not subject to a moral restriction.
A Comparative Study of Various Deep Learning Approaches to Shape Encoding of Planar Geospatial Objects
Authors: Yan Xiongfeng , Yang Min
Published: October 2022

The shape encoding of geospatial objects is a key problem in the fields of cartography and geoscience. Although traditional geometric-based methods have made great progress, deep learning techniques offer a development opportunity for this classical problem. In this study, a shape encoding framework based on a deep encoder–decoder architecture was proposed, and three different methods for encoding planar geospatial shapes, namely GraphNet, SeqNet, and PixelNet methods, were constructed based on raster-based, graph-based, and sequence-based modeling for shape. The three methods were compared with the existing deep learning-based shape encoding method and two traditional geometric methods. Quantitative evaluation and visual inspection led to the following conclusions: (1) The deep encoder–decoder methods can effectively compute shape features and obtain meaningful shape coding to support the shape measure and retrieval task. (2) Compared with the traditional Fourier transform and turning function methods, the deep encoder–decoder methods showed certain advantages. (3) Compared with the SeqNet and PixelNet methods, GraphNet performed better due to the use of a graph to model the topological relations between nodes and efficient graph convolution and pooling operations to process the node features.
Time-Space Compression Effect of High-Speed Rail on Tourist Destinations in China
Authors: Li Taohong , Shi Hong , Chen Ning Chris , Yang Luo
Published: October 2022

This study proposes a time-space compression (TSC) model and evaluates the TSC effect of high-speed rail (HSR) on a sample of 2662 classified tourist destinations from 2008 to 2019 in China with the help of GIS technology. Based on panel models, this study finds that, within five hours: (1) the TSC effect of HSR on tourist destinations in eastern and central China is three times stronger than that in western and north-eastern China; (2) the negative impact coefficient of TSC of HSR on tourist destination development in China within temporal distances (3 h, 4 h, 5 h) are −0.193, −0.117, and −0.091 respectively; and (3) the farther the temporal distance, the weaker the inhibitory effect. Results from this study contribute to the literature by providing empirical evidence of the potentially negative TSC effect on regional and tourism development. Findings provide managerial implications suggesting that tourist destinations should implement marketing policies to retain tourists and prevent the loss of tourists brought by the opening of HSR.
ST3DNetCrime: Improved ST-3DNet Model for Crime Prediction at Fine Spatial Temporal Scales
Authors: Dong Qifen , Li Yu , Zheng Ziwan , Wang Xun , Li Guojun
Published: October 2022

Crime prediction is crucial for sustainable urban development and protecting citizens’ quality of life. However, there exist some challenges in this regard. First, the spatio-temporal correlations in crime data are relatively complex and are heterogenous in time and space, hence it is difficult to model the spatio-temporal correlation in crime data adequately. Second, crime prediction at fine spatial temporal scales can be applied to micro patrol command; however, crime data are sparse in both time and space, making crime prediction very challenging. To overcome these challenges, based on the deep spatio-temporal 3D convolutional neural networks (ST-3DNet), we devise an improved ST-3DNet framework for crime prediction at fine spatial temporal scales (ST3DNetCrime). The framework utilizes diurnal periodic integral mapping to solve the problem of sparse and irregular crime data at fine spatial temporal scales. ST3DNetCrime can, respectively, capture the spatio-temporal correlations of recent crime data, near historical crime data and distant historical crime data as well as describe the difference in the correlations’ contributions in space. Extensive experiments on real-world datasets from Los Angeles demonstrated that the proposed ST3DNetCrime framework has better prediction performance and enhanced robustness compared with baseline methods. In additon, we verify that each component of ST3DNetCrime is helpful in improving prediction performance.
Mapping and Validation of BrGOLDEN: A Dominant Gene Regulating Carotenoid Accumulation in Brassica rapa
Authors: Zhang Lei , Zhang Shifan , Dai Yun , Wang Shaoxing , Wang Chenggang , Li Fei , Zhang Hui , Chen Guohu , Yuan Lingyun , Hou Jinfeng , Tang Xiaoyan , Zhu Shidong , Sun Rifei , Li Guoliang , Zhang Shujiang
Published: October 2022

In plants, the accumulation of carotenoids can maintain the balance of the photosystem and improve crop nutritional quality. Therefore, the molecular mechanisms underlying carotenoid synthesis and accumulation should be further explored. In this study, carotenoid accumulation differed significantly among parental Brassica rapa. Genetic analysis was carried out using the golden inner leaf ‘1900264′ line and the light−yellow inner leaf ‘1900262′ line, showing that the golden inner leaf phenotype was controlled by a single dominant gene. Using bulked−segregant analysis sequencing, BraA09g007080.3C encoding the ORANGE protein was selected as a candidate gene. Sequence alignment revealed that a 4.67 kb long terminal repeat insertion in the third exon of the BrGOLDEN resulted in three alternatively spliced transcripts. The spatiotemporal expression results indicated that BrGOLDEN might regulate the expression levels of carotenoid−synthesis−related genes. After transforming BrGOLDEN into Arabidopsis thaliana, the seed−derived callus showed that BrGOLDENIns and BrGOLDENDel lines presented a yellow color and the BrGOLDENLdel line presented a transparent phenotype. In addition, using the yeast two−hybrid assay, BrGOLDENIns, BrGOLDENLdel, and Brgoldenwt exhibited strong interactions with BrPSY1, but BrGOLDENDel did not interact with BrPSY1 in the split−ubiquitin membrane system. In the secondary and 3D structure analysis, BrGOLDENDel was shown to have lost the PNFPSFIPFLPPL sequences at the 125 amino acid position, which resulted in the α−helices of BrGOLDENDel being disrupted, restricting the formation of the 3D structure and affecting the functions of the protein. These findings may provide new insights into the regulation of carotenoid synthesis in B. rapa.
Etiology of IBD—Is It Still a Mystery?
Authors: Kofla-Dłubacz Anna , Pytrus Tomasz , Akutko Katarzyna , Sputa-Grzegrzółka Patrycja , Piotrowska Aleksandra , Dzięgiel Piotr
Published: October 2022

Inflammatory bowel diseases (IBD), including colitis ulcerosa and Crohn’s disease, are chronic diseases of the gastrointestinal tract for which the cause has not been fully understood. However, it is known that the etiology is multifactorial. The multidirectional network of interactions of environmental, microbiological and genetic factors in predisposed persons lead to an excessive and insufficiently inhibited reaction of the immune system, leading to the development of chronic inflammation of the gastrointestinal walls, the consequence of which is the loss of the function that the intestine performs, inter alia, through the process of fibrosis. Detailed knowledge of the pathways leading to chronic inflammation makes it possible to pharmacologically modulate disorders and effectively treatthese diseases. In this review, we described the primary and adaptive immune system response in the gut and the known immune pathogenetic pathways leading to the development of IBD. We also described the process leading to intestinal tissue fibrosis, which is an irreversible consequence of untreated IBD.
Identification of a Maturation Plasma Cell Index through a Highly Sensitive Droplet Digital PCR Assay Gene Expression Signature Validation in Newly Diagnosed Multiple Myeloma Patients
Authors: Martello Marina , Solli Vincenza , Termini Rosalinda , Kanapari Ajsi , Remondini Daniel , Borsi Enrica , Poletti Andrea , Armuzzi Silvia , Taurisano Barbara , Vigliotta Ilaria , Mazzocchetti Gaia , Zamagni Elena , Merlotti Alessandra , Tacchetti Paola , Pantani Lucia , Rocchi Serena , Rizzello Ilaria , Mancuso Katia , Cavo Michele , Terragna Carolina
Published: October 2022

DNA microarrays and RNA-based sequencing approaches are considered important discovery tools in clinical medicine. However, cross-platform reproducibility studies undertaken so far have highlighted that microarrays are not able to accurately measure gene expression, particularly when they are expressed at low levels. Here, we consider the employment of a digital PCR assay (ddPCR) to validate a gene signature previously identified by gene expression profile. This signature included ten Hedgehog (HH) pathways’ genes able to stratify multiple myeloma (MM) patients according to their self-renewal status. Results show that the designed assay is able to validate gene expression data, both in a retrospective as well as in a prospective cohort. In addition, the plasma cells’ differentiation status determined by ddPCR was further confirmed by other techniques, such as flow cytometry, allowing the identification of patients with immature plasma cells’ phenotype (i.e., expressing CD19+/CD81+ markers) upregulating HH genes, as compared to others, whose plasma cells lose the expression of these markers and were more differentiated. To our knowledge, this is the first technical report of gene expression data validation by ddPCR instead of classical qPCR. This approach permitted the identification of a Maturation Index through the integration of molecular and phenotypic data, able to possibly define upfront the differentiation status of MM patients that would be clinically relevant in the future.
The OsCBL8–OsCIPK17 Module Regulates Seedling Growth and Confers Resistance to Heat and Drought in Rice
Authors: Gao Cong , Lu Shuai , Zhou Rong , Wang Zihui , Li Yi , Fang Hui , Wang Baohua , Chen Moxian , Cao Yunying
Published: October 2022

The calcium signaling pathway is critical for plant growth, development, and response to external stimuli. The CBL–CIPK pathway has been well characterized as a calcium-signaling pathway. However, in most reports, only a single function for this module has been described. Here, we examined multiple functions of this module. CIPK showed a similar distribution to that of CBL, and OsCBL and OsCIPK families were retained after experiencing whole genome duplication events through the phylogenetic and synteny analysis. This study found that OsCBL8 negatively regulated rice seed germination and seedling growth by interacting with OsCIPK17 with overexpression and gene editing mutant plants as materials combining plant phenotype, physiological indicators and transcriptome sequencing. This process is likely mediated by OsPP2C77, which is a member of the ABA signaling pathway. In addition, OsCBL mediated the targeting of OsNAC77 and OsJAMYB by OsCIPK17, thus conferring resistance to high temperatures and pathogens in rice. Our work reveals a unique signaling pathway, wherein OsCBL8 interacts with OsCIPK17 and provides rice with multiple resistance while also regulating seedling growth.
Assessing the DNA Damaging Effectiveness of Ionizing Radiation Using Plasmid DNA
Authors: Maayah Yara , Nusrat Humza , Pang Geordi , Tambasco Mauro
Published: October 2022

Plasmid DNA is useful for investigating the DNA damaging effects of ionizing radiation. In this study, we have explored the feasibility of plasmid DNA-based detectors to assess the DNA damaging effectiveness of two radiotherapy X-ray beam qualities after undergoing return shipment of ~8000 km between two institutions. The detectors consisted of 18 μL of pBR322 DNA enclosed with an aluminum seal in nine cylindrical cavities drilled into polycarbonate blocks. We shipped them to Toronto, Canada for irradiation with either 100 kVp or 6 MV  X-ray beams to doses of 10, 20, and 30 Gy in triplicate before being shipped back to San Diego, USA. The Toronto return shipment also included non-irradiated controls and we kept a separate set of controls in San Diego. In San Diego, we quantified DNA single strand breaks (SSBs), double strand breaks (DSBs), and applied Nth and Fpg enzymes to quantify oxidized base damage. The rate of DSBs/Gy/plasmid was 2.8±0.7 greater for the 100 kVp than the 6 MV irradiation. The 100 kVp irradiation also resulted in 5±2 times more DSBs/SSB than the 6 MV beam, demonstrating that the detector is sensitive enough to quantify relative DNA damage effectiveness, even after shipment over thousands of kilometers.
Mother–Fetus Immune Cross-Talk Coordinates “Extrinsic”/“Intrinsic” Embryo Gene Expression Noise and Growth Stability
Authors: Babochkina Tatyana Ivanovna , Gerlinskaya Ludmila Alekseevna , Anisimova Margarita Vladimirovna , Kontsevaya Galina Vladimirovna , Feofanova Natalia Aleksandrovna , Stanova Aliya Konstantinovna , Moshkin Mikhail Pavlovich , Moshkin Yuri Mikhailovich
Published: October 2022

Developmental instability (DI) is thought to be inversely related to a capacity of an organism to buffer its development against random genetic and environmental perturbations. DI is represented by a trait’s inter- and intra-individual variabilities. The inter-individual variability (inversely referred to as canalization) indicates the capability of organisms to reproduce a trait from individual to individual. The intra-individual variability reflects an organism’s capability to stabilize a trait internally under the same conditions, and, for symmetric traits, it is expressed as fluctuating asymmetry (FA). When representing a trait as a random variable conditioned on environmental fluctuations, it is clear that, in statistical terms, the DI partitions into “extrinsic” (canalization) and “intrinsic” (FA) components of a trait’s variance/noise. We established a simple statistical framework to dissect both parts of a symmetric trait variance/noise using a PCA (principal component analysis) projection of the left/right measurements on eigenvectors followed by GAMLSS (generalized additive models for location scale and shape) modeling of eigenvalues. The first eigenvalue represents “extrinsic” and the second—“intrinsic” DI components. We applied this framework to investigate the impact of mother–fetus major histocompatibility complex (MHC)-mediated immune cross-talk on gene expression noise and developmental stability. We showed that “intrinsic” gene noise for the entire transcriptional landscape could be estimated from a small subset of randomly selected genes. Using a diagnostic set of genes, we found that allogeneic MHC combinations tended to decrease “extrinsic” and “intrinsic” gene noise in C57BL/6J embryos developing in the surrogate NOD-SCID and BALB/c mothers. The “intrinsic” gene noise was negatively correlated with growth (embryonic mass) and the levels of placental growth factor (PLGF), but not vascular endothelial growth factor (VEGF). However, it was positively associated with phenotypic growth instability and noise in PLGF. In mammals, the mother–fetus MHC interaction plays a significant role in development, contributing to the fitness of the offspring. Our results demonstrate that a positive impact of distant MHC combinations on embryonic growth could be mediated by the reduction of “intrinsic” gene noise followed by the developmental stabilization of growth.
Rapid Removal of Toxic Remazol Brilliant Blue-R Dye from Aqueous Solutions Using Juglans nigra Shell Biomass Activated Carbon as Potential Adsorbent: Optimization, Isotherm, Kinetic, and Thermodynamic Investigation
Authors: Parimelazhagan Vairavel , Yashwath Pranesh , Arukkani Pushparajan Dharun , Carpenter Jitendra
Published: October 2022

Recently, the treatment of effluent by agricultural waste biomass has significantly attracted wide interest among researchers due to its availability, efficacy, and low cost. The removal of toxic Remazol Brilliant Blue-R (RBBR) from aqueous solutions using HNO3-treated Juglans nigra (walnut) shell biomass carbon as an adsorbent has been examined under various experimental conditions, such as initial pH, adsorbate concentration, adsorbent dosage, particle size, agitation speed, and type of electrolyte. The experiments are designed to achieve the maximum dye removal efficiency using the response surface methodology (RSM). The optimum pH, adsorbent dosage, and particle size were found to be 1.5, 7 g L−1, and 64 μm, respectively for maximum decolorization efficiency (98.24%). The prepared adsorbent was characterized by particle size, Brunauer–Emmett–Teller (BET) surface area, pore volume, zero-point charge (pHzpc), Fourier transform infrared spectroscopy (FT-IR), field emission scanning electron microscopy/energy dispersive X-ray spectroscopy (FE-SEM/EDX), X-ray diffraction (XRD), and thermogravimetric analysis (TGA). Based on fitting the experimental data with various models, the isotherm and kinetic mechanism are found to be more appropriate with Langmuir isotherm and pseudo-second-order kinetics. The adsorption mechanism can be described by the intra-particle diffusion model, Bangham, and Boyd plots. The overall rate of adsorption is controlled by the external film diffusion of dye molecules. The maximum monolayer adsorption capacity, (qmax) 54.38 mg g−1 for RBBR dye, was obtained at a temperature of 301 K. From a thermodynamic standpoint, the process is endothermic, spontaneous, and the chemisorption process is favored at high temperatures. Desorption studies were conducted with various desorbing reagents in various runs and the maximum desorption efficiency (61.78% in the third run) was obtained using the solvent methanol. Reusability studies demonstrated that the prepared adsorbent was effective for up to three runs of operation. The investigation outcomes concluded that walnut shell biomass activated carbon (WSBAC) is a cost-effective, eco-friendly, and bio-sustainable material that can be used for synthetic dye decolorization in aqueous media.
The Citrus Flavonoid Nobiletin Downregulates Angiopoietin-like Protein 3 (ANGPTL3) Expression and Exhibits Lipid-Modulating Effects in Hepatic Cells and Adult Zebrafish Models
Authors: Lin Ching-Yen , Chen Pei-Yi , Hsu Hao-Jen , Gao Wan-Yun , Wu Ming-Jiuan , Yen Jui-Hung
Published: October 2022

Nobiletin, a dietary citrus flavonoid, exerts biological activities against hyperlipidemia, obesity, and atherosclerotic cardiovascular diseases (ASCVDs). The aim of this study was to explore the lipid-lowering effects of nobiletin and the underlying molecular mechanisms in vitro in hepatic cells and in vivo in zebrafish models. Transcriptome and gene ontology (GO) analyses of differentially expressed genes (DEGs) by gene set enrichment analysis (GSEA) showed that a set of twenty-eight core enrichment DEGs associated with “GO BP regulation of lipid metabolic process” (GO: 0019216) were significantly downregulated in nobiletin-treated cells. Among these genes, angiopoietin-like 3 (ANGPTL3), an inhibitor of lipoprotein lipase (LPL) activity that regulates TG-rich lipoprotein (TGRL) metabolism in circulation, was the protein most markedly downregulated by nobiletin. Nobiletin (20 and 40 μM) significantly reduced the levels of ANGPTL3 mRNA and intracellular and secreted ANGPTL3 proteins in hepatic cell lines. Furthermore, alleviation of secreted ANGPTL3 production by nobiletin was found to reinstate LPL catalytic activity. Nobiletin significantly inhibited ANGPTL3 promoter activity and attenuated the transcription factor liver X receptor-α (LXRα)-mediated ANGPTL3 transcription. Molecular docking analysis predicted that nobiletin could bind to the ligand-binding domain of LXRα, thereby counteracting LXRα activation. In animal studies, orally administered nobiletin significantly alleviated the levels of plasma triglycerides (TGs) and cholesterol in zebrafish fed a high-fat diet. Moreover, nobiletin significantly reduced the amounts of hepatic ANGPTL3 protein in zebrafish. Our findings suggest that nobiletin may regulate the LXRα-ANGPTL3-LPL axis and exhibit lipid-modulating effects in vitro and in vivo. Thus, nobiletin is a potential ANGPTL3 inhibitor for the regulation of lipid metabolism to ameliorate dyslipidemia and ASCVDs.
Molecular Mechanisms of Diverse Auxin Responses during Plant Growth and Development
Authors: Zhang Yang , Yu Jiajie , Xu Xiuyue , Wang Ruiqi , Liu Yingying , Huang Shan , Wei Hairong , Wei Zhigang
Published: October 2022

The plant hormone auxin acts as a signaling molecule to regulate numerous developmental processes throughout all stages of plant growth. Understanding how auxin regulates various physiological and developmental processes has been a hot topic and an intriguing field. Recent studies have unveiled more molecular details into how diverse auxin responses function in every aspect of plant growth and development. In this review, we systematically summarized and classified the molecular mechanisms of diverse auxin responses, and comprehensively elaborated the characteristics and multilevel regulation mechanisms of the canonical transcriptional auxin response. On this basis, we described the characteristics and differences between different auxin responses. We also presented some auxin response genes that have been genetically modified in plant species and how their changes impact various traits of interest. Finally, we summarized some important aspects and unsolved questions of auxin responses that need to be focused on or addressed in future research. This review will help to gain an overall understanding of and some insights into the diverse molecular mechanisms of auxin responses in plant growth and development that are instrumental in harnessing genetic resources in molecular breeding of extant plant species.
The Manufacture of Xeno- and Feeder-Free Clinical-Grade Human Embryonic Stem Cell Lines: First Step for Cell Therapy
Authors: Souralova Tereza , Rehakova Daniela , Jeseta Michal , Tesarova Lenka , Beranek Jindrich , Ventruba Pavel , Hampl Ales , Koutna Irena
Published: October 2022

Human embryonic stem cells (hESCs) are increasingly used in clinical trials as they can change the outcome of treatment for many human diseases. They are used as a starting material for further differentiation into specific cell types and to achieve the desirable result of the cell therapy; thus, the quality of hESCs has to be taken into account. Therefore, current good manufacturing practice (cGMP) has to be implemented in the transport of embryos, derivation of inner cell mass to xeno-free, feeder-free and defined hESC culture, and cell freezing. The in-depth characterization of hESC lines focused on safety, pluripotency, differentiation potential and genetic background has to complement this process. In this paper, we show the derivation of three clinical-grade hESC lines, MUCG01, MUCG02, and MUCG03, following these criteria. We developed and validated the system for the manufacture of xeno-free and feeder-free clinical-grade hESC lines that present high-quality starting material suitable for cell therapy according to cGMP.
RETRACTED: Sun et al. WDN: A One-Stage Detection Network for Wheat Heads with High Performance. Information 2022, 13, 153
Authors: Sun Pengshuo , Cui Jingyi , Hu Xuefeng , Wang Qing
Published: October 2022

The journal retracts the article “WDN: A One-Stage Detection Network for Wheat Heads with High Performance” [...]
Fuzzy Spatiotemporal Data Modeling and Operations in RDF
Authors: Zhu Lin , Meng Xiangfu , Mi Zehui
Published: October 2022

With the emergence of a large number of fuzzy spatiotemporal data on the Web, how to represent and operate fuzzy spatiotemporal data has become an important research issue. Meanwhile, the Resource Description Framework (RDF) is a standard data and knowledge description language of the Semantic Web and has been applied in many application areas, such as geographic information systems and meteorological systems. In this paper, a model for representing fuzzy spatiotemporal data is proposed and a set of algebraic operations for the model are investigated. First, a representation method of fuzzy spatiotemporal RDF data and a fuzzy spatiotemporal RDF graph model are proposed. In addition, a formal fuzzy spatiotemporal RDF algebra is proposed and a set of algebraic operations for manipulating fuzzy spatiotemporal RDF data are developed. The algebraic operations include: set operation, selection operation, projection operation, join operation, and construction operation. Finally, the existing SPARQL query language is extended and an example that shows how to apply the proposed algebraic operations to capture the queries expressed by the extended SPARQL query language is given.