bluuebunny/crossref_metadata_2025 huggingface dataset:
Synthesis and Anti-Melanoma Activity of L-Cysteine-Coated Iron Oxide Nanoparticles Loaded with Doxorubicin
Authors: Toderascu Luiza Izabela , Sima Livia Elena , Orobeti Stefana , Florian Paula Ecaterina , Icriverzi Madalina , Maraloiu Valentin-Adrian , Comanescu Cezar , Iacob Nicusor , Kuncser Victor , Antohe Iulia , Popescu-Pelin Gianina , Stanciu George , Ionita Petre , Mihailescu Cristian N. , Socol Gabriel
Published: February 2023

In this study, we report on the synthesis of L-Cysteine (L-Cys)-coated magnetic iron oxide nanoparticles (NPs) loaded with doxorubicin (Dox). The Fe3O4-L-Cys-Dox NPs were extensively characterized for their compositional and morpho-structural features using EDS, SAED, XRD, FTIR and TEM. XPS, Mӧssbauer spectroscopy and SQUID measurements were also performed to determine the electronic and magnetic properties of the Fe3O4-L-Cys-Dox nanoparticles. Moreover, by means of a FO-SPR sensor, we evidenced and confirmed the binding of Dox to L-Cys. Biological tests on mouse (B16F10) and human (A375) metastatic melanoma cells evidenced the internalization of magnetic nanoparticles delivering Dox. Half maximum inhibitory concentration IC50 values of Fe3O4-L-Cys-Dox were determined for both cell lines: 4.26 µg/mL for A375 and 2.74 µg/mL for B16F10, as compared to 60.74 and 98.75 µg/mL, respectively, for unloaded controls. Incubation of cells with Fe3O4-L-Cys-Dox modulated MAPK signaling pathway activity 3 h post-treatment and produced cell cycle arrest and increased apoptosis by 48 h. We show that within the first 2 h of incubation in physiological (pH = 7.4) media, ~10–15 µM Dox/h was released from a 200 µg/mL Fe3O4-L-Cys-Dox solution, as compared to double upon incubation in citrate solution (pH = 3), which resembles acidic environment conditions. Our results highlight the potential of Fe3O4-L-Cys-Dox NPs as efficient drug delivery vehicles in melanoma therapy.
Airbrushed PSF/ZnO Composite Coatings as a Novel Approach for the Consolidation of Historical Bones
Authors: Moradienayat Monireh , González-Benito Javier , Olmos Dania
Published: February 2023

In this work, the preparation and characterization of films based on polysulfone (PSF) filled with zinc oxide, ZnO, nanoparticles (NPs) are conducted. The novelty of this research mainly relies on two points: (i) the use of a commercial airbrush to prepare or modify materials, and (ii) the design of new materials (nanocomposites) for the consolidation and restoration of historical bones. To accomplish these objectives, free-standing thin films and ancient bone coatings of PSF/ZnO nanocomposites with different particle contents (0%, 1%, 2%, 5% and 10%, % wt) are prepared using a commercial airbrush. Mechanical characterization is carried out to correlate properties between free-standing thin films and coatings, thus understanding the final performance of the coatings as consolidants for ancient bones. Thin films of PSF/ZnO show that the elastic modulus (E) increases with particle content. The mechanical behavior of the surfaces of the treated and untreated bones is studied locally using Martens hardness measurements. Maximum values of Martens hardness are obtained for the bone samples treated with polysulfone filled with 1% ZnO nanoparticles (HM = 850 N·mm−2) or 2% ZnO (HM = 625 N·mm−2) compared to those treated just with neat PSF (HM = 282 N·mm−2) or untreated bone (HM = 140 N·mm−2), indicating there is a correspondence between rigidity of free-standing films and hardness of the corresponding coatings. In terms of mechanical performance, it is demonstrated the existence of a balance between nanoparticle concentration and probability of nanoparticle aggregation, which allows better material design for ancient bones consolidation.
Broadband Plasmonic Metamaterial Optical Absorber for the Visible to Near-Infrared Region
Authors: Musa Ahmad , Alam Touhidul , Islam Mohammad Tariqul , Hakim Mohammad Lutful , Rmili Hatem , Alshammari Ahmed S. , Islam Md. Shabiul , Soliman Mohamed S.
Published: February 2023

An oblique angle and polarization insensitive metamaterial absorber (MA) are highly desired for the visible and infrared optical applications like, wave energy harvesting, optical filters, and detecting thermal leaks and electrical defects. In this paper, a multi-layered MA consisting of two layers of tungsten resonators on a silicon dioxide substrate, coated with additional SiO2 materials is investigated. The unit cell size of the MA is 0.5λ × 0.5λ × 0.8λ, at the lowest wavelength. The proposed MA offers an average absorption of 92% from 400 nm to 2400 nm with stable oblique incident angles up to 45°. The structure also achieves polarization insensitivity at the entire visible and near-infrared spectrum. Moreover, the MA is found highly compatible for solar absorber applications with high y AAM1.5. The structure is also compatible for filter application in optical communication system by modifying the plasmonic nano structure. The modified structure can block the wavelengths of the visible band (450 nm to 800 nm) and transmit optical communication bands (800 to 1675 nm). These versatile absorption and filtering performance make the proposed design highly potential for solar energy harvesting, photodetection, thermal imaging, photo-trapping, and optical communications applications.
Numerical Investigation of GaN HEMT Terahertz Detection Model Considering Multiple Scattering Mechanisms
Authors: Meng Qingzhi , Lin Qijing , Wang Zelin , Wang Yangtao , Jing Weixuan , Xian Dan , Zhao Na , Yao Kun , Zhang Fuzheng , Tian Bian , Jiang Zhuangde
Published: February 2023

GaN high-electron-mobility transistor (HEMT) terahertz (THz) detectors have been widely studied and applied in the past few decades. However, there are few reports about the influence of GaN/AlGaN heterostructure material properties on the detection model at present. In this paper, a response voltage model for a GaN HEMT THz detector that considers the carrier scattering in a GaN/AlGaN heterostructure is proposed. The phonon scattering, dislocation scattering, and interface roughness scattering mechanisms are taken into account in the classic THz response voltage model; furthermore, the influence of various material parameters on the response voltage is studied. In a low-temperature region, acoustic scattering plays an important role, and the response voltage drops with an increase in temperature. In a high temperature range, optical phonon scattering is the main scattering mechanism, and the detector operates in a non-resonant detection mode. With an increase in carrier surface density, the response voltage decreases and then increases due to piezoelectric scattering and optical phonon scattering. For dislocation and interface roughness scattering, the response voltage is inversely proportional to the dislocation density and root mean square roughness (RMS) but is positively related to lateral correlation length. Finally, a comparison between our model and the reported models shows that our proposed model is more accurate.
Performance of Cu/ZnO Nanosheets on Electrospun Al2O3 Nanofibers in CO2 Catalytic Hydrogenation to Methanol and Dimethyl Ether
Authors: Maor Itzhak , Heyte Svetlana , Elishav Oren , Mann-Lahav Meirav , Thuriot-Roukos Joelle , Paul Sébastien , Grader Gideon
Published: February 2023

The synthesis of methanol and dimethyl ether (DME) from carbon dioxide (CO2) and green hydrogen (H2) offers a sustainable pathway to convert CO2 emissions into value-added products. This heterogeneous catalytic reaction often uses copper (Cu) catalysts due to their low cost compared with their noble metal analogs. Nevertheless, improving the activity and selectivity of these Cu catalysts for these products is highly desirable. In the present study, a new architecture of Cu- and Cu/Zn-based catalysts supported on electrospun alumina nanofibers were synthesized. The catalysts were tested under various reaction conditions using high-throughput equipment to highlight the role of the hierarchical fibrous structure on the reaction activity and selectivity. The Cu or Cu/ZnO formed a unique structure of nanosheets, covering the alumina fiber surface. This exceptional morphology provides a large surface area, up to ~300 m2/g, accessible for reaction. Maximal production of methanol (~1106 gmethanolKgCu−1∙h−1) and DME (760 gDMEKgCu−1∙h−1) were obtained for catalysts containing 7% wt. Cu/Zn with a weight ratio of 2.3 Zn to Cu (at 300 °C, 50 bar). The promising results in CO2 hydrogenation to methanol and DME obtained here point out the significant advantage of nanofiber-based catalysts in heterogeneous catalysis.
Global Dietary and Herbal Supplement Use during COVID-19—A Scoping Review
Authors: Arora Ishaan , White Shecoya , Mathews Rahel
Published: February 2023

During the first year of the COVID-19 pandemic, the lack of cure and the intensity of the global spread raised a common awareness of health. The aim of this scoping review is to summarize dietary supplement use globally during first two years of the COVID-19 pandemic. A systematic search was conducted in December 2021 following PRISMA guidelines. PubMed, ERIC, and Scopus databases were searched, and 956 results were screened for eligibility. Fourteen cross-sectional studies from 11 countries and 3 continents were examined. All studies were large population surveys investigating healthy eating and supplement use during COVID-19. Vitamin C, vitamin D, zinc and multivitamins were the most widely reported, as well as natural/herbal products such as ginger and honey. The most common reason cited for supplements use was to strengthen immune system and to prevent infection of COVID-19. These studies reported that populations are relying on healthcare providers, family, friends, and social media to learn about supplement use. Future studies on the treatment of COVID-19 should include more evidence for supplement use.
Mushroom-Based Supplements in Italy: Let’s Open Pandora’s Box
Authors: Risoli Samuele , Nali Cristina , Sarrocco Sabrina , Cicero Arrigo Francesco Giuseppe , Colletti Alessandro , Bosco Filippo , Venturella Giuseppe , Gadaleta Agata , Gargano Maria Letizia , Marcotuli Ilaria
Published: February 2023

Mushrooms and derivates are well known to the scientific community for having different health benefits and exhibit a wide range of pharmacological activities, including lipid-lowering, antihypertensive, antidiabetic, antimicrobic, antiallergic, anti-inflammatory, anticancer, immunomodulating, neuroprotective and osteoprotective actions. In Europe, medical mushrooms are mainly marketed in the form of food supplements as single components or combined with other nutraceuticals. In this context, the first peculiarity that distinguishes it is the safety established through the “history of consumption” that characterizes that mushroom. However, the cultivation of medicinal mushrooms on a large scale is performed mainly in China, where most of the production facilities do not have internationally recognized good manufacturing practices, despite that many European companies that sell myotherapies are supplied by Chinese manufacturers. This is particularly evident in Italy, where an arsenal of mushroom products is marketed in the form of powders and extracts not always of ascertained origin and sometimes of doubtful taxonomic identification, and thus not meeting the quality criteria required. The growing interest in mycotherapy involves a strong commitment from the scientific community to propose supplements of safe origin and genetic purity as well as to promote clinical trials to evaluate its real effects on humans. The purpose of this research is to analyze different mushroom-based dietary supplements used in medicine as monotherapy on the Italian market and to evaluate their composition and quality. The molecular identification of the sequences with those deposited in GenBank allowed for identifying 6 out of 19 samples, matching with those deposited belonging to the species indicated in the label, i.e., Lentinula edodes (samples 1, 4, 12 and 18) and Ganoderma lucidum (samples 5 and 10). Samples containing Ganoderma, labeled in the commercial product as G. lucidum, showed sequences that showed homology of 100% and 99% with G. resinaceum and G. sichuanense. An additional investigation was carried out in order to determine the active fungal ingredients, such as ergosterol, aflatoxins, heavy metals, nicotine and total glucan. The results obtained and shown in the manuscript highlight how the data were not only in line with what is expected with respect to what is indicated in the labels.
A Taxonomy-Agnostic Approach to Targeted Microbiome Therapeutics—Leveraging Principles of Systems Biology
Authors: Brumfield Kyle D. , Cox Paul , Geyer James , Goepp Julius
Published: February 2023

The study of human microbiomes has yielded insights into basic science, and applied therapeutics are emerging. However, conflicting definitions of what microbiomes are and how they affect the health of the “host” are less understood. A major impediment towards systematic design, discovery, and implementation of targeted microbiome therapeutics is the continued reliance on taxonomic indicators to define microbiomes in health and disease. Such reliance often confounds analyses, potentially suggesting associations where there are none, and conversely failing to identify significant, causal relationships. This review article discusses recent discoveries pointing towards a molecular understanding of microbiome “dysbiosis” and away from a purely taxonomic approach. We highlight the growing role of systems biological principles in the complex interrelationships between the gut microbiome and host cells, and review current approaches commonly used in targeted microbiome therapeutics, including fecal microbial transplant, bacteriophage therapies, and the use of metabolic toxins to selectively eliminate specific taxa from dysbiotic microbiomes. These approaches, however, remain wholly or partially dependent on the bacterial taxa involved in dysbiosis, and therefore may not capitalize fully on many therapeutic opportunities presented at the bioactive molecular level. New technologies capable of addressing microbiome-associated diseases as molecular problems, if solved, will open possibilities of new classes and categories of targeted microbiome therapeutics aimed, in principle, at all dysbiosis-driven disorders.
Status of Infectious Diseases in Free-Ranging European Brown Hares (Lepus europaeus) Found Dead between 2017 and 2020 in Schleswig-Holstein, Germany
Authors: Faehndrich Marcus , Klink Jana C. , Roller Marco , Wohlsein Peter , Raue Katharina , Strube Christina , Prenger-Berninghoff Ellen , Ewers Christa , Capucci Lorenzo , Lavazza Antonio , Tomaso Herbert , Schnitzler Joseph G. , Siebert Ursula
Published: February 2023

The European brown hare (Lepus europaeus) is a quite adaptable species, but populations have been decreasing for several decades in different countries, including Germany. To investigate infectious diseases as possible influences on observed population decline in the German federal state Schleswig-Holstein, 118 deceased free-ranging European brown hares were collected between 2017 and 2020 and underwent detailed postmortem examination with extended sampling. Infectious diseases were a major cause of death (34.7%). The number of juveniles found exceeded the adult ones. The main pathomorphological findings were hepatitis (32.8%), pneumonia (22.2%), nephritis (19.1%), liver necrosis (12.9%), and enteritis (40.7%). An unusual main finding was steatitis (20.9%) of unknown origin. Animals were mainly emaciated and showed high infection rates with Eimeria spp. (91.3%) and Trichostrongylus spp. (36.2%). European Brown Hare Syndrome Virus reached an epidemic status with few fatal infections (4.2%) and high seroprevalence (64.9%), whereas the prevalence of Rabbit Haemorrhagic Disease Virus 2 was very low (0.8%) in hares in Schleswig-Holstein. Pathogens such as Yersinia pseudotuberculosis (5.9%), Pasteurella multocida (0.8%), and Staphylococcus aureus (3.4%) only caused sporadic deaths. This study illustrates the wide distribution of various infectious pathogens with high mortality and even zoonotic potential. Infectious diseases need to be considered as an important influence on population dynamics in Schleswig-Holstein.
The DarT/DarG Toxin–Antitoxin ADP-Ribosylation System as a Novel Target for a Rational Design of Innovative Antimicrobial Strategies
Authors: Catara Giuliana , Caggiano Rocco , Palazzo Luca
Published: February 2023

The chemical modification of cellular macromolecules by the transfer of ADP-ribose unit(s), known as ADP-ribosylation, is an ancient homeostatic and stress response control system. Highly conserved across the evolution, ADP-ribosyltransferases and ADP-ribosylhydrolases control ADP-ribosylation signalling and cellular responses. In addition to proteins, both prokaryotic and eukaryotic transferases can covalently link ADP-ribosylation to different conformations of nucleic acids, thus highlighting the evolutionary conservation of archaic stress response mechanisms. Here, we report several structural and functional aspects of DNA ADP-ribosylation modification controlled by the prototype DarT and DarG pair, which show ADP-ribosyltransferase and hydrolase activity, respectively. DarT/DarG is a toxin–antitoxin system conserved in many bacterial pathogens, for example in Mycobacterium tuberculosis, which regulates two clinically important processes for human health, namely, growth control and the anti-phage response. The chemical modulation of the DarT/DarG system by selective inhibitors may thus represent an exciting strategy to tackle resistance to current antimicrobial therapies.
Screening the Pathogen Box to Discover and Characterize New Cruzain and TbrCatL Inhibitors
Authors: do Valle Moreira Thales , Martins Luan Carvalho , Diniz Lucas Abreu , Bernardes Talita Cristina Diniz , de Oliveira Renata Barbosa , Ferreira Rafaela Salgado
Published: February 2023

Chagas disease and Human African Trypanosomiasis, caused by Trypanosoma cruzi and T. brucei, respectively, pose relevant health challenges throughout the world, placing 65 to 70 million people at risk each. Given the limited efficacy and severe side effects associated with current chemotherapy, new drugs are urgently needed for both diseases. Here, we report the screening of the Pathogen Box collection against cruzain and TbrCatL, validated targets for Chagas disease and Human African Trypanosomiasis, respectively. Enzymatic assays were applied to screen 400 compounds, validate hits, determine IC50 values and, when possible, mechanisms of inhibition. In this case, 12 initial hits were obtained and ten were prioritized for follow-up. IC50 values were obtained for six of them (hit rate = 1.5%) and ranged from 0.46 ± 0.03 to 27 ± 3 µM. MMV687246 was found to be a mixed inhibitor of cruzain (Ki = 57 ± 6 µM) while MMV688179 was found to be a competitive inhibitor of cruzain with a nanomolar potency (Ki = 165 ± 63 nM). A putative binding mode for MMV688179 was obtained by docking. The six hits discovered against cruzain and TbrCatL are of great interest for further optimization by the medicinal chemistry community.
Molecular Detection and Phylogenetic Analysis of Riemerella anatipestifer in Poultry and Wild Geese in Poland
Authors: Sawicka-Durkalec Anna , Tomczyk Grzegorz , Gerilovych Iryna , Kursa Olimpia
Published: February 2023

Riemerella anatipestifer (RA) is one of the most relevant bacterial pathogens of commercial waterfowl from clinical and economic points of view. Our study aimed to evaluate the prevalence of RA infection in different types of commercial poultry in Poland and verify the potential role of wild geese as vectors of this pathogen. We tested a total of 126 poultry flocks, including geese (N = 20), ducks (N = 42), turkeys (N = 64) and 19 wild geese, including greater white-fronted geese (N = 9), greylag geese (N = 5) and Taiga bean geese (N = 5). Tracheal swabs were examined for RA using a PCR targeting a conserved region of the 16S rRNA gene. Selected PCR products were sequenced to perform the phylogenetic analysis. Among the commercial poultry, the highest RA prevalence was found in flocks of ducks (35.7%) and geese (30.0%), whereas the lowest one was found in turkeys (3.2%). Most tested wild geese (94.7%) were RA positive. The phylogenetic analysis showed relatively low genetic diversity of the sequences analyzed, which gathered in two clusters of the phylogenetic tree, and the minimum nucleotide identity was 98.6%. Our results would support the contention that RA isolates from commercial poultry circulate in wild bird populations but are not transmitted back to poultry.
Identification of Pathogenic Leptospira kirschneri Serogroup Grippotyphosa in Water Voles (Arvicola terrestris) from Ruminant Pastures in Puy-de-Dôme, Central France
Authors: Harran Elena , Pinot Adrien , Kodjo Angeli , Djelouadji Zouheira , Le Gudayer Marine , Sionfoungo Daouda Soro , Groud Karine , Lattard Virginie , Ayral Florence
Published: February 2023

Rodents are the primary reservoirs for pathogenic Leptospira species, which cause leptospirosis. Among the key potential carriers are water voles, whose population outbreaks can consequently pose a major threat to human and animal health. We studied the prevalence, prominence, and epidemiology of pathogenic Leptospira species in water voles in central France. First, 46 voles were captured, and DNA was extracted from kidney, lung, liver, blood, and urine and tested for the presence of Leptospira using three molecular methods: PCR, O-antigen typing, and variable number tandem repeat (VNTR) typing. We also attempted to culture leptospires from kidney and urine samples. In addition, we investigated leptospiral antibodies in serum samples from 60 sheep using microscopic agglutination testing. These animals co-occurred with the voles, so we sought to assess their degree of exposure and involvement in pathogen dynamics. The overall prevalence of infection was 76.1% (CI95% [61.2%, 87.4%]). The only strain found was L. kirschneri serogroup Grippotyphosa and a similar VNTR profile was acquired. Leptospires were successfully cultured from kidney and urine samples for four voles. Three sheep had low antibody titers against the Leptospira serogroup Grippotyphosa. Taken together, our results suggest the exclusive carriage of L. kirschneri serogroup Grippotyphosa among water voles in central France. Nevertheless, their ability to act as reservoir hosts that transmit the pathogen to co-occurring livestock remains unclear and merits further research.
Time-Varying Engineered Reservoir for the Improved Estimation of Atom-Cavity Coupling Strength
Authors: Xia Ye , Guo Weiming , Miao Zibo
Published: February 2023

In this paper, we consider the application of quantum reservoir engineering in quantum metrology. More precisely, we are concerned with a system setup where a sequence of atoms constructing the “time-varying” quantum reservoir interact, in turn, with the trapped field in a cavity through the Jaynes–Cummings Hamiltonian. In particular, we were able to manipulate the initial states of reservoir atoms in order to enhance estimation precision regarding the coupling strength between each atom and the cavity (the coupling strength between each atom and the cavity was assumed to be identical). The novelty of this work lies in alternately preparing the atoms at two different states in a pairwise manner, such that the cavity could converge into a squeezed state with photonic loss to the environment taken into account. The control scheme proposed here thus leads to higher precision compared to the previous work where reservoir atoms were initialized at the same state, which drove the cavity to a coherent state. Detailed theoretical analysis and numerical simulations are also provided. In addition, this system setup and the associated control scheme are easily implemented for quantum metrology, since no entanglement is required for the preparation of atom states, and the final cavity state can stay steady.
Diffusion in Phase Space as a Tool to Assess Variability of Vertical Centre-of-Mass Motion during Long-Range Walking
Authors: Boulanger Nicolas , Buisseret Fabien , Dehouck Victor , Dierick Frédéric , White Olivier
Published: February 2023

When a Hamiltonian system undergoes a stochastic, time-dependent anharmonic perturbation, the values of its adiabatic invariants as a function of time follow a distribution whose shape obeys a Fokker–Planck equation. The effective dynamics of the body’s centre-of-mass during human walking is expected to represent such a stochastically perturbed dynamical system. By studying, in phase space, the vertical motion of the body’s centre-of-mass of 25 healthy participants walking for 10 min at spontaneous speed, we show that the distribution of the adiabatic invariant is compatible with the solution of a Fokker–Planck equation with a constant diffusion coefficient. The latter distribution appears to be a promising new tool for studying the long-range kinematic variability of walking.
Antifungal In Vitro Activity of Phoradendron sp. Extracts on Fungal Isolates from Tomato Crop
Authors: Salas-Gómez Alma Leticia , Espinoza Ahumada César Alejandro , Castillo Godina Rocío Guadalupe , Ascacio-Valdés Juan Alberto , Rodríguez-Herrera Raúl , Segura Martínez Ma. Teresa de Jesús , Neri Ramírez Efraín , Estrada Drouaillet Benigno , Osorio-Hernández Eduardo
Published: February 2023

Synthetic chemicals are mainly used for the control of fungal diseases in tomato, causing the phytopathogens to generate resistance to the chemical active ingredient, with a consequent risk to human health and the environment. The use of plant extracts is an option for the control of these diseases, which is why the main objective of this research was to study an alternative biocontrol strategy for the management of plant diseases caused by fungi through obtaining polyphenol extracts from mistletoe plants growing on three different tree species—mesquite (Prosopis glandulosa), cedar (Cedrus), and oak (Quercus), which contain flavones, anthocyanins, and luteolin. The overall chemical structure of the obtained plant extracts was investigated by RP-HPLC-ESI-MS liquid chromatography. The antifungal effect of these extracts was examined. The target phytopathogenic fungi were isolated from tomato plantations located in Altamira, Tamaulipas, Mexico. The microorganisms were characterized by classical and molecular methods and identified as Alternaria alternata, Fusarium oxysporum, Fusarium sp., and Rhizoctonia solani.
Assessing Effect of Rootstock Micropropagation on Field Performance of Grafted Peach Varieties by Fitting Mixed-Effects Models: A Longitudinal Study
Authors: Marín Juan A. , García Elena , Lorente Pilar , Andreu Pilar , Arbeloa Arancha
Published: February 2023

Rootstock micropropagation has been extensively used as an alternative to propagation by cuttings. Although studies have recently been conducted on other species, no conclusive reports have been published on the effect of rootstock micropropagation on the field performance of fruit trees. Here, we present the results of a five-year study of peach varieties grafted on two rootstocks (Adesoto 101 and Adafuel), either micropropagated or propagated by cuttings, to ascertain the effect of the rootstock propagation method on field performance. Fruit trees are woody plants with a long life cycle; so, to reduce the influence of environmental or cultural factors on the agronomical results, studies need to last for several years, in which data are obtained from the same individuals over time (longitudinal data). This hinders the analysis because these data lack independence. In contrast with a more traditional approach with data aggregation and repeated-measures ANOVA analysis, in this study, we used linear mixed-effects models to control the variance associated with random factors without data aggregation. The growth of the fruit trees did not appreciably differ between the rootstock propagation methods, neither in the flowering period nor in the yield. The models constructed for different parameters of the field performance (trunk cross-sectional area (TCSA), cumulative yield, cumulative yield efficiency, and cumulative crop load) showed a very good fit (R2 > 0.97), allowing the conclusion that the rootstock propagation method did not affect the field performance of fruit trees in this study.
LG5, a Novel Allele of EUI1, Regulates Grain Size and Flag Leaf Angle in Rice
Authors: Li Zhen , Liu Junrong , Wang Xingyu , Wang Jing , Ye Junhua , Xu Siliang , Zhang Yuanyuan , Hu Dongxiu , Zhang Mengchen , Xu Qun , Wang Shan , Yang Yaolong , Wei Xinghua , Feng Yue , Wang Shu
Published: February 2023

Grain size and flag leaf angle are two important traits that determining grain yield in rice. However, the mechanisms regulating these two traits remain largely unknown. In this study, a rice long grain 5 (lg5) mutant with a large flag leaf angle was identified, and map-based cloning revealed that a single base substitution followed by a 2 bp insertion in the LOC_Os05g40384 gene resulted in larger grains, a larger flag leaf angle, and higher plant height than the wild type. Sequence analysis revealed that lg5 is a novel allele of elongated uppermost internode-1 (EUI1), which encodes a cytochrome P450 protein. Functional complementation and overexpression tests showed that LG5 can rescue the bigger grain size and larger flag leaf angle in the Xiushui11 (XS) background. Knockdown of the LG5 transcription level by RNA interference resulted in elevated grain size and flag leaf angle in the Nipponbare (NIP) background. Morphological and cellular analyses suggested that LG5 regulated grain size and flag leaf angle by promoting cell expansion and cell proliferation. Our results provided new insight into the functions of EUI1 in rice, especially in regulating grain size and flag leaf angle, indicating a potential target for the improvement of rice breeding.
Exploration and Comparison of the Behavior of Some Indigenous and International Varieties (Vitis vinifera L.) Grown in Climatic Conditions of Herzegovina: The Influence of Variety and Vintage on Physico-Chemical Characteristics of Grapes
Authors: Jovanović-Cvetković Tatjana , Sredojević Milica , Natić Maja , Grbić Rada , Akšić Milica Fotirić , Ercisli Sezai , Cvetković Miljan
Published: February 2023

Viticulture is of great economic importance in the southern part of Bosnia and Herzegovina, thanks to favorable climatic conditions and a long-standing tradition of growing vines. The assortment is dominated by international varieties, as well as some autochthonous and domesticated varieties. The subject of the research is the analysis of the quality of Cabernet Sauvignon, Merlot, Vranac, and Blatina varieties at two localities in Herzegovina during the period 2020–2021. The paper examined the most important economic and technological characteristics, grape quality, and berry phenolic profile. A particularly pronounced variation of the tested characteristics during the research period was observed in the Merlot and Blatina varieties, while the Cabernet Sauvignon and Vranac varieties showed a slightly higher stability of the tested characteristics. Poorer grape quality during the research period was registered with the Blatina variety, which can be considered a varietal characteristic to some extent. The analyzed grape varieties were rich in polyphenols, and the impact of grape variety on the berry phenolic profiles was confirmed. The most abundant polyphenols in the analyzed grape samples were quercetin 3-O-glucoside and catechin gallate, followed by kaempferol 3-O-glucoside. The highest values of polyphenols were found mainly in the samples originating from Trebinje. Indigenous Balkan grape varieties (Vranac and Blatina) stood out with particularly high contents of some phenolics. Research has shown that climatic conditions have a significant influence on the most important characteristics of grapes, which are conditioned by genotypic specificities. The conditions for growing vines in the conditions of Herzegovina enable high quality in the production of grapes, especially the Cabernet Sauvignon and Vranac varieties. The autochthonous variety Blatina shows significant variations in grape quality during the test period, which was confirmed by the results of a larger number of studies in the previous period.
Structure−Activity Relationship (SAR) Study of trans-Cinnamic Acid and Derivatives on the Parasitic Weed Cuscuta campestris
Authors: Moreno-Robles Antonio , Cala Peralta Antonio , Zorrilla Jesús G. , Soriano Gabriele , Masi Marco , Vilariño-Rodríguez Susana , Cimmino Alessio , Fernández-Aparicio Mónica
Published: February 2023

Cuscuta campestris Yunck. is a parasitic weed responsible for severe yield losses in crops worldwide. The selective control of this weed is scarce due to the difficult application of methods that kill the parasite without negatively affecting the infected crop. trans-Cinnamic acid is secreted by plant roots naturally into the rhizosphere, playing allelopathic roles in plant–plant communities, although its activity in C. campestris has never been investigated. In the search for natural molecules with phytotoxic activity against parasitic weeds, this work hypothesized that trans-cinnamic acid could be active in inhibiting C. campestris growth and that a study of a series of analogs could reveal key structural features for its growth inhibition activity. In the present structure–activity relationship (SAR) study, we determined in vitro the inhibitory activity of trans-cinnamic acid and 24 analogs. The results showed that trans-cinnamic acid’s growth inhibition of C. campestris seedlings is enhanced in eight of its derivatives, namely hydrocinnamic acid, 3-phenylpropionaldehyde, trans-cinnamaldehyde, trans-4-(trifluoromethyl)cinnamic acid, trans-3-chlorocinnamic acid, trans-4-chlorocinnamic acid, trans-4-bromocinnamic acid, and methyl trans-cinnamate. Among the derivatives studied, the methyl ester derivative of trans-cinnamic acid was the most active compound. The findings of this SAR study provide knowledge for the design of herbicidal treatments with enhanced activity against parasitic weeds.
Intercropping of Cyphomandra betacea with Different Ploidies of Solanum Sect. Solanum (Solanaceae) Wild Vegetables Increase Their Selenium Uptakes
Authors: Lin Lijin , Xu Xiangting , Wang Jin , Wang Xun , Lv Xiulan , Tang Yi , Deng Honghong , Liang Dong , Xia Hui
Published: February 2023

Selenium (Se) deficiency causes various diseases in humans. Se can be obtained from fruits and vegetables. In this study, the fruit tree Cyphomandra betacea was intercropped with three Solanum sect. Solanum (Solanaceae) wild vegetables [diploid (S. photeinocarpum), tetraploid (colchicine-induced S. photeinocarpum), and hexaploid (S. nigrum)], respectively, and Se uptakes of these plants were determined by a pot experiment. Intercropping decreased the biomass, photosynthetic pigment content, and superoxide dismutase activity of C. betacea, but increased the peroxidase (POD) activity, catalase (CAT) activity, and soluble protein content of C. betacea. These indicators’ values of sect. Solanum increased after intercropping. The contents of Se increased in C. betacea and sect. Solanum after intercropping. Intercropped with diploid, tetraploid, and hexaploid increased the shoot Se contents in C. betacea by 13.73%, 17.49%, and 26.50%, respectively, relative to that of C. betacea monoculture. Intercropped with C. betacea increased the shoot Se contents in diploid, tetraploid, and hexaploid by 35.22%, 68.86%, and 74.46%, respectively, compared with their respective monoculture. The biomass and Se content of intercropped sect. Solanum showed linear relationships with the biomass and Se content of their monocultures. The biomass and Se content of intercropped C. betacea also exhibited linear relationships with that of sect. Solanum monocultures. Correlation and grey relational analyses revealed that the CAT activity, POD activity, and soluble protein content were the top three indicators closely associated with the C. betacea shoot Se content. The POD activity, soluble protein content, and translocation factor were the top three indicators closely associated with sect. Solanum shoot Se content. Therefore, intercropping can promote the Se uptake in C. betacea and sect. Solanum wild vegetables.
Coating Materials to Increase the Stability of Liposomes
Authors: Pasarin Diana , Ghizdareanu Andra-Ionela , Enascuta Cristina Emanuela , Matei Catalin Bogdan , Bilbie Catalin , Paraschiv-Palada Luciana , Veres Petronela-Andreea
Published: February 2023

Liposomes carry various compounds with applications in pharmaceutical, food, and cosmetic fields, and the administration route is especially parenteral, oral, or transdermal. Liposomes are used to preserve and release the internal components, thus maintaining the properties of the compounds, the stability and shelf life of the encapsulated products, and their functional benefits. The main problem in obtaining liposomes at the industrial level is their low stability due to fragile phospholipid membranes. To increase the stability of liposomes, phospholipid bilayers have been modified or different coating materials have been developed and studied, both for liposomes with applications in the pharmaceutical field and liposomes in the food field. In the cosmetic field, liposomes need no additional coating because the liposomal formulation is intended to have a fast penetration into the skin. The aim of this review is to provide current knowledge regarding physical and chemical factors that influence stability, coating materials for liposomes with applications in the pharmaceutical and food fields to increase the stability of liposomes containing various sensitive compounds, and absorption of the liposomes and commercial liposomal products obtained through various technologies available on the market.
A Novel Parallel Simulated Annealing Methodology to Solve the No-Wait Flow Shop Scheduling Problem with Earliness and Tardiness Objectives
Authors: Karacan Ismet , Senvar Ozlem , Bulkan Serol
Published: February 2023

In this paper, the no-wait flow shop problem with earliness and tardiness objectives is considered. The problem is proven to be NP-hard. Recent no-wait flow shop problem studies focused on familiar objectives, such as makespan, total flow time, and total completion time. However, the problem has limited studies with solution approaches covering the concomitant use of earliness and tardiness objectives. A novel methodology for the parallel simulated annealing algorithm is proposed to solve this problem in order to overcome the runtime drawback of classical simulated annealing and enhance its robustness. The well-known flow shop problem datasets in the literature are utilized for benchmarking the proposed algorithm, along with the classical simulated annealing, variants of tabu search, and particle swarm optimization algorithms. Statistical analyses were performed to compare the runtime and robustness of the algorithms. The results revealed the enhancement of the classical simulated annealing algorithm in terms of time consumption and solution robustness via parallelization. It is also concluded that the proposed algorithm could outperform the benchmark metaheuristics even when run in parallel. The proposed algorithm has a generic structure that can be easily adapted to many combinatorial optimization problems.
Conceptual Process Design to Produce Bio-Acrylic Acid via Gas Phase Dehydration of Lactic Acid Produced from Carob Pod Extracts
Authors: Ortiz Martínez Víctor M. , Saavedra María I. , Salar García María J. , Godínez Carlos , Lozano-Blanco Luis J. , Sanchez-Segado Sergio
Published: February 2023

This work discusses the conceptual process design for the integrated production of bio-based acrylic acid from carob pod aqueous extracts. CHEMCAD was used for the process simulation and cost estimation of the relevant equipment. The process was designed for a capacity of 68 kt of carob pod per year, operating 8000 h annually, and involving extraction, fermentation, catalytic dehydration, and distillation to achieve 99.98%w/w acrylic acid as the main product. The economic assessment for the base case suggests a fixed capital investment of EUR 62.7 MM with an internal rate of return of 15.8%. The results obtained show that carob pod is a promising biomass source for the production of bio-acrylic acid.
Resilience in Supply and Demand Networks
Authors: Klementzki Vanessa , Glistau Elke , Trojahn Sebastian , Coello Machado Norge Isaias Coello
Published: February 2023

The present era is characterised by many events that have influences on supply chains and supply networks. This concerns, e.g., war, epidemics, natural disasters, accidents, strikes, political instability, and political sanctions. These are generally grouped under the term “disruption”. In order to avoid the risk of supply chain disruption, major disruption of supply networks, or loss of customers associated with disruptions, it is necessary to take preventive and proactive measures in supply chain management in terms of planning. This paper is intended to briefly summarise the current state of knowledge with the most important facts and derive a new definition from it. In addition, an analogy to maintenance is established for the first time. In doing so, a comparison of the concepts and a listing of the important proactive measures derived from them for increasing resilience are made. In the course of this, the field of action considered is extended from the exchange of suppliers through the entire supply chain network to the exchange of customers.
Same-Day Digital Dentistry Restorative Workflow for Single Immediate Provisionalization of Narrow-Diameter Implants: An Exploratory Prospective Study
Authors: Deeb Janina Golob , Reddy Nitya G. , Hopfensperger Liam J. , Harris April L. , Bencharit Sompop
Published: February 2023

This study evaluated the two-year clinical outcomes of 3.1 mm diameter dental implants, immediately provisionalized and later restored using same-day dentistry, in 10 patients receiving 11 narrow-diameter (3.1 mm) single implants. Each implant was placed and immediately restored with a provisional crown after placement. At least 2 months after placement, the implant was restored with a prefabricated titanium abutment and an all-ceramic crown using a same-day dentistry protocol. Clinical outcomes, including apical bone loss, probing depths, gingival index, and surgical and prosthetic complications, were documented. There was no implant failure over the course of two years. No surgical complications were reported. Two cases lost provisional crowns. One crown needed to be remade due to esthetic concern. The cumulative two-year survival rate of the implants was 100%. Implant bone loss after two years of functional loading was −0.56 ± 0.54 mm and −0.32 ± 0.68 mm for mesial and distal crestal bone, respectively. Two prosthetic complications included recementation of a crown and remaking of a crown. This exploratory study suggests that immediate provisionalization and a same-day restorative dentistry digital workflow protocol for narrow-diameter implants appear to be predictable clinical procedures with no reported surgical complications and minimal prosthetic complications.
Sustainability 3.0 in Libraries: A Challenge for Management
Authors: Keller Alice
Published: February 2023

This article discusses three questions: “How can libraries make an effective contribution to resolving the sustainability challenges we are collectively facing?”; “When are libraries truly sustainable?”; and “How can library management support this shift?”. Looking across libraries and their history over the last few decades, the author discerns different stages of development leading to sustainability. In line with the work of Dyllick and Muff the author describes Sustainability Levels 0.0 to 3.0. The highest level requires a quantum leap and shifts from thinking inside out to thinking outside in. This article addresses the need that there is virtually no academic management literature on the topic of sustainability in libraries. It shows that whilst there are many examples of individual projects or activities, there is a serious lack of methodology at the senior management level.
Comparing Moralities in the Abrahamic and Indic Religions Using Cognitive Science: Kindness, Peace, and Love versus Justice, Violence, and Hate
Authors: Nakissa Aria
Published: February 2023

Recent cognitive science research indicates that humans possess numerous biologically rooted religious and moral intuitions. The present article draws on this research to compare forms of religious morality in the Abrahamic traditions (Judaism, Christianity, Islam) and the Indic traditions (Hinduism, Buddhism, Jainism). Special attention is given to moral teachings on kindness, peace, and love, as well as related teachings on justice, violence, and hate. The article considers how moral intuitions shape Abrahamic/Indic moral teachings, which, in turn, impact: (1) Abrahamic/Indic doctrines concerning politics, law, and war; (2) Abrahamic/Indic doctrines concerning individual ethics, and moral behavior proper to monastics and laypersons; and (3) Abrahamic/Indic doctrines concerning theological matters, such as the nature of the universe, souls, and deities.
Aristotelian-Thomistic Contribution to the Contemporary Studies on Biological Life and Its Origin
Authors: Tabaczek Mariusz
Published: February 2023

The phenomenon of life belongs to the most intriguing and puzzling aspects of reality, studied in various divisions of natural science, as well as in philosophy and theology. The purpose of this article is twofold. Firstly, it aims at bringing into the rich contemporary conversation on the nature, origin, and persistence of life a deeper and more thorough insight coming from the classical Aristotelian-Thomistic philosophy of nature and metaphysics. Secondly, in reference to the theological aspects of the debate, the article presents the two contrasting positions on the necessity of a direct divine intervention in the origin of life and analyzes them from the same Aristotelian-Thomistic perspective.