bluuebunny/crossref_metadata_2025 huggingface dataset:
Dyadic relationships of people with schizophrenia
Authors: Stusiński Jarosław , Merk Wojciech , Lew-Starowicz Michal
Published: October 2022

Tworzenie udanych związków partnerskich ma duże znaczenie dla ogólnej jakości życia. Osoby chorujące na schizofrenię doświadczają znacznych trudności w zawieraniu i utrzymywaniu związków diadycznych ze względu na objawy psychotyczne, konsekwencje choroby i jej leczenia czy stygmatyzację społeczną. Trudności w sferze tworzenia intymnych relacji dostrzegane są już w okresie dorastania, stanowiąc jeden z elementów zmian prepsychotycznych. Wśród osób z rozpoznaniem schizofrenii, kobiety częściej niż mężczyźni tworzą związki diadyczne, co może wynikać z późniejszego początku choroby, lepszych wskaźników funkcjonowania społecznego oraz bardziej sprzyjających wzorców społeczno-kulturowych. Wśród pacjentów będących w związkach, jakość ich relacji ma duże znaczenie dla przebiegu choroby i leczenia. Osoby chorujące na schizofrenię często preferują tworzenie związków z innymi chorymi ze względu na możliwość stworzenia wyrównanej relacji zapewniającej akceptację i wsparcie. Zdrowi partnerzy osób ze schizofrenią, ze względu na obciążenia wynikające ze specyfiki choroby i zaangażowanie w opiekę nad chorym partnerem, także potrzebują profesjonalnego wsparcia. Kwestie dotyczące związków diadycznych powinny być uwzględniane w holistycznym podejściu terapeutycznym do osób z rozpoznaniem schizofrenii.
Physical activity as a therapeutic method for non-pharmacological treatment of schizophrenia: A systematic literature review
Authors: Cempa Katarzyna , Jurys Tomasz , Kluczyński Sebastian , Andreew Martyna
Published: October 2022

Cel

Celem niniejszego systematycznego przeglądu była klasyfikacja, porównanie i scharakteryzowanie wybranych rodzajów aktywności fizycznych mających pozytywny wpływ na przebieg procesu leczenia schizofrenii (w tym jej skutków odległych).

Materiał i metody

Przegląd literatury na potrzeby niniejszej pracy został przeprowadzony w bazach naukowych: PubMed/MEDLINE, Web of Science oraz EBSCO. Analiza i dalszy opis został opracowany na podstawie protokołu PRISMA.

Wyniki

Podczas analizy baz danych wyszukano 330 pozycji potencjalnych źródeł wiedzy do przygotowania przeglądu literatury w temacie aktywności fizycznej wykorzystywanej w leczeniu schizofrenii. Po procesie weryfikacji i kwalifikacji do badania zostało włączonych 17 pozycji.

Wnioski

Włączenie aktywności fizycznej do procesu leczenia chorych na schizofrenię wykazywało pozytywny wpływ na odczuwane objawy i dolegliwości związane z przebiegiem choroby oraz wspomagało powrót chorych do społeczeństwa.
The self-disclosure of mental health problems: Its extent, predictors and consequences for people with psychotic disorders
Authors: Sonik-Włodarczyk Janina , Anczewska Marta , Grygiel Paweł , Stefaniak Izabela , Świtaj Piotr
Published: October 2022

Cel pracy

Analiza zakresu, społeczno-demograficznych i klinicznych predyktorów oraz konsekwencji ujawniania problemów ze zdrowiem psychicznym przez osoby z zaburzeniami psychotycznymi.

Metoda

147 osób z rozpoznaniem zaburzeń psychotycznych (kategorie F20-F29 wg ICD-10) zostało zbadanych za pomocą kwestionariuszy do oceny zakresu i konsekwencji ujawniania informacji o problemach psychicznych, funkcjonowania społecznego, objawów depresyjnych i globalnego nasilenia objawów psychopatologicznych.

Wyniki

Większość respondentów otwarcie mówiła o swoich problemach ze zdrowiem psychicznym rodzicom, małżonkom lub partnerom życiowym oraz lekarzom i innym pracownikom niepsychiatrycznej opieki zdrowotnej, natomiast zdecydowana mniejszość (mniej niż jedna piąta) – przypadkowo poznanym osobom, sąsiadom, nauczycielom i wykładowcom, współpracownikom, policjantom i strażnikom miejskim, przedstawicielom wymiaru sprawiedliwości oraz urzędnikom. Analiza regresji wielokrotnej wykazała, że im ankietowani byli starsi, tym mniej chętnie ujawniali swoje problemy psychiczne innym osobom (β = –0,34; p < 0,05). Z kolei im dłużej chorowali, tym bardziej byli skłonni do ujawniania swoich problemów psychicznych (β = 0,29; p < 0,05). Ujawnienie informacji o problemach ze zdrowiem psychicznym miało zróżnicowany wpływ na relacje społeczne badanych – znaczna część nie dostrzegała różnicy w sposobie traktowania przez innych, inni zgłaszali pogorszenie lub poprawę w tym zakresie.

Wnioski

Wyniki badania dostarczają klinicystom praktycznych wskazówek odnośnie wspomagania i wspierania pacjentów z zaburzeniami psychotycznymi w procesie świadomego podejmowania decyzji o „wychodzeniu z ukrycia”.
Letter to the Editor. Psychiatric emergency associated with depressive disorders
Authors: Szulc Agata , Gałecki Piotr , Samochowiec Jerzy , Dudek Dominika
Published: October 2022

bez streszczenia
Addison’s disease presenting as shortness of breath and hypotension in early pregnancy
Authors: Avery Williams  , Blair Buschmann  , Rimu Afrina , Jennifer Phy
Published: October 2022

Background: Addison’s disease is an adrenal cortex disorder that is considered rare and the pathophysiology is not well understood. There are very few cases reported during pregnancy.
Case: Our patient is a 30-year-old previously healthy female that presented to the Reproductive Endocrinology and Infertility clinic at 7 weeks and 2 days pregnant with shortness of breath. After further evaluation, she was noted to have symptoms that led to the differential diagnosis of Addison’s disease. The patient’s 21 hydroxylase antibody was positive which along with the other tests, confirmed the diagnosis. Endocrinology started the patient on hydrocortisone and fludrocortisone.
Conclusion: There is more research that needs to be done but the rarity of this disorder makes this case important for understanding Addison’s disease and its treatment.
Machine learning and medicine- a brief introduction
Authors: Lee Benjamin , Peterson Christopher
Published: October 2022

Artificial Intelligence (AI) and Machine Learning (ML) have advanced rapidly in recent years and now have the potential to change medicine. This review serves as an introduction to AI and the potential it has to change medicine. Specific examples of past milestones particularly in the domain of critical care are presented, including algorithms that have been developed that can interpret chest x-rays or predict clinical outcomes such as extubation failure or ICU mortality. Included is a brief general discussion of what AI is, how it is made, and how physicians will be involved with it. Arguments are then presented as to why AI will likely not leave physicians without a job, including expectations vs. reality, AI still requires human supervision, new discoveries bring new challenges, and AI cannot design itself. Far from displacing physicians, AI, if implemented well, stands poised to automate repetitive tasks, make physicians more accurate, and free them up to spend more time with patients.
Bilateral infraclavicular brachial plexus nerve blocks in an ambulatory surgery center
Authors: Rachman Benjamin , Zuercher Hannah , Jones Kristina , Rachman Nathan
Published: October 2022

The approach for upper extremity surgery brachial plexus blocks depends on the specific nature of the surgery. Interscalene and supraclavicular brachial plexus blocks can cause phrenic nerve palsy. Our aim was to explore the safety of bilateral parasagittal infraclavicular brachial plexus blocks (BPBBs) in an outpatient surgery center. Identical BPBBs were performed in two patients with 20 mL of 0.25% bupivacaine. Neither patient developed respiratory or cardiovascular distress. Brachial plexus blocks on multiple locations are infrequently employed for fear of phrenic nerve paresis. However, given both patients’ success, bilateral parasagittal infraclavicular brachial plexus blocks may provide a safe approach. Keywords: Nerve block, brachial plexus blocks, phrenic nerve, anesthesia, pain
The mental health impact of work from home: a literature review
Authors: Sarangi Ashish , Kim Dalynn , Rafael John
Published: October 2022

The 2020 coronavirus (COVID-19) pandemic has shifted the workplace focus from on-site to remote and has introduced discussions about the positive and negative features of working from home (WFH). Many employees have reported increased control and flexibility regarding one’s schedule with the shift to a remote model. However, there have been increasing concerns regarding the emotional and mental health effects of such a model and the social isolation resulting from staying at home. The lack of professional boundaries, technological limitations, and forced interaction with family members have been considered potentials costs of the convenience of WFH. In this review paper, we discuss the possible benefits and consequences of remote work on various measures of mental health and discuss the implications of future WFH models which may provide workers with greater autonomy and flexibility.
Iatrogenic opioid withdrawal syndrome presentation and considerations
Authors: Evans Abbie , Nugent Kenneth
Published: October 2022

The 2018 Critical Care Medicine Guidelines for Analgesia and Sedation in the ICU Good Practice Statement emphasized the importance in the treatment of pain taking precedence over sedation. Within these guidelines, a multimodal analgesia approach is standard of care in the ICU, which often includes the initiation of opioids. 1 As practice has shifted to prioritize pain management over sedation, opioids have been administered in frequent and prolonged dosages, making the critically ill patient susceptible to iatrogenic opioid withdrawal syndrome (IOWS). This article provides an overview of IOWS to alert clinicians to the diagnosis of IOWS in ICU patients.
Monitoring sedation during mechanical ventilation
Authors: Motes Arunee
Published: October 2022

Sedative medications have been used in intensive care units (ICU) to minimize discomfort, prevent pain /anxiety, allow invasive procedures, reduce stress, and improve synchrony in mechanically ventilated (MV) patients. However, these drugs can have adverse effects resulting in increased length of ICU/hospital stay, health care costs, morbidity, and mortality.  This review summarizes the mechanisms of action, usual doses, side effects, adverse effects, contraindications, and recent studies of sedatives which are frequently used in adult ICUs and the sedation assessment tools for measuring quality and depth of sedation in adult ICU patients.
 
Keywords sedations, mechanical ventilation, intensive care unit, sedation assessment tools
Clinical effectiveness of generic vancomycin products compared to Vancocin CP® in patients with methicillin-resistant Staphylococcus aureus infections: A retrospective cohort
Authors: Castaño-Rendón Juliana , Sánchez López Sebastián , Padierna Andrés , Palacio María , Tobon Laura , Ossa María , Muriel Carolina , Tello Johana , Jaimes Fabián
Published: October 2022

Introduction: Approval of generic drugs requires only bioequivalence studies. Some research suggests that generic vancomycin is not clinically equivalent to the branded drug, and this exposes patients to therapeutic failure and the development of microbial resistance.
Aims: Compare the rates of microbiological and clinical failure between generic vancomycin and Vancocin-CP®.
Methods: Retrospective cohort analysis of hospitalized adults with culture-proven methicillin-resistant Staphylococcus aureus infection, treated with vancomycin in a tertiary care hospital in Medellín, Colombia. General clinical variables, laboratory findings, severity and mortality scores, and type of vancomycin used were recorded. Logistical regression models, adjusted for potential confounders, were fitted to estimate the effect of vancomycin on clinical and microbiologic outcomes.
Results: Of 209 patients, 153 (73.2%) received generic vancomycin and 56 (26.8%) Vancocin-CP®. Systems more commonly affected were skin and soft tissues (28.5%), blood with involvement of catheters (27.6%) and blood without the involvement of catheters (23.3%). There were 62 clinical failures (29.5%) and 41(38%) microbiological failures. The hospital mortality rate was 15% (n = 31); only 7 (3.4%) episodes of adverse drug reactions were documented. No difference was found in the risk of clinical or microbiological failure between Vancocin-CP® and generic products with OR = 2.3 (95% CI = 0.8; 6.3) and 0.89 (95% CI = 0.4; 1.9), respectively. 
Conclusion: There were no association between the use of generic vancomycin and the outcomes of clinical or microbiological failure. Sample size is an important limitation for these findings.
 
Keywords: Vancomycin, bioequivalence, MRSA, clinical equivalence
Collagenous gastritis presenting as chronic heartburn in a patient with psoriatic arthritis
Authors: Deb Anasua , Songtanin Busara , Thongtan Thanita , Islam Sameer
Published: October 2022

Collagenous gastritis is a rare cause of heartburn in adults. Histopathological examination of gastric mucosal biopsy from the stomach shows submucosal collagen deposition. The pathophysiologic mechanism is unknown, and collagenous gastritis cases have been associated with certain drugs, such as olmesartan and non-steroidal anti-inflammatory drugs, and certain medical conditions, such as common variable immunodeficiency, primary IgM deficiency, autoimmune disorders, and psoriatic arthropathy. Here we report a case of collagenous gastritis in a 29-year-old woman with psoriatic arthropathy who presented with persistent heartburn. She was successfully treated with oral pantoprazole.
 
Key words: heartburn, collagenous gastritis,
Understanding Inclusive Education in the “Front of the Class” Movie in Chinese Perspective
Authors: Tan Qinyi , Liu Wan , Teng Luyan
Published: October 2022

To explore the beliefs of Inclusive Education in the film " Front of the class ", this study uses the method of content analysis to interpret and analyze this film from the perspective of the concept of inclusive education, inclusive family, inclusive school, and inclusive society. It found that the film contains rich beliefs and concepts of inclusive education, which helps to pursue the quality of education examine educational equity and social equity, provide intellectual support for the construction of inclusive society, responding the UN's sustainable development goal 4 (SDG 4) focuses on ensuring inclusive and equitable, quality education and lifelong learning opportunities for all.
Funding Black-led micro-organisations in England
Authors: Manful Adwoa , Willis Rosalind
Published: October 2022

This article presents an approach aimed at dealing with the difficulties faced by Black-led micro-organisations in England when accessing longer-term funding. The experiences of Black-led micro-organisations working in partnership with a mainstream organisation and a mid-level minority ethnic organisation are reported in the article. The participants of the research attributed the Black-led micro-organisations’ difficulties in accessing funds to institutional racism. To overcome this, the micro-organisations received targeted funding through a partnership that not only makes funding more accessible, but also builds the capacity of the Black-led micro-organisations. This was because a micro-organisation on its own or as a group of micro-organisations would not have been successful in a funding application. The formation of the three-tier partnership came with many challenges, but ultimately with the building of trust by skilled leadership and the right individuals, the Black-led micro-organisations were able to access sustainable long-term funding.
Catalyst for change: Lessons learned from overcoming barriers to providing safe abortion care in Médecins Sans Frontières projects
Authors: Kumar Manisha , Schulte‐Hillen Catrin , De Plecker Eva , Van Haver Ann , Marques Sonia Guinovart , Daly Maura , Vochten Hilde , Merzaghi Lisa , de le Vingne Brice , Saint‐Sauveur Jean François
Published: October 2022

Context

Despite instituting a policy in 2004, Médecins Sans Frontières (MSF) continuously struggled to routinely provide safe abortion care (SAC). In 2016, the organization launched an initiative aimed at increasing availability of SAC in MSF projects and increasing understanding of abortion‐related dynamics in humanitarian settings.

Methodology

From March 2017 to April 2018, MSF staff conducted support visits to 10 projects in a country in sub‐Saharan Africa. Each visit followed a systematic approach with six key components and related tools that were later shared with teams worldwide. Data regarding women seeking abortion services and related outcomes were collected and analyzed retrospectively.

Results

From Q1 2017 through Q4 2019, SAC provision increased significantly in all 10 projects, rising from three to 759 safe abortions per quarter. Teams received 3831 patients seeking SAC and provided 3640 first and second trimester abortions, over 99% via medication methods. The overall complication rate was 4.29% and 0.3% for severe, life‐threatening complications. No major security incidents were reported. MSF provision of SAC worldwide increased from 781 in 2016 (the year before this initiative began) to 21,546 in 2019.

Conclusion

Implementation of SAC in humanitarian settings—even those with significant legal restrictions—is possible and necessary. Both first and second trimester medication abortion can be safely and effectively provided through both home‐ and facility‐based models of care. Programmatic data provide valuable insights into abortion‐related dynamics which must shape operational decision‐making. Addressing internal barriers and providing direct field support were key to stimulating organizational cultural change.
Surgical Versus Conservative Management for Treating Unstable Atlas Fractures: A Multicenter Study
Authors: Shin Jun Jae , Kim Kwang-Ryeol , Shin Joongkyum , Kang Jiin , Lee Ho Jin , Kim Tae Woo , Hong Jae Taek , Kim Sang-Woo , Ha Yoon
Published: October 2022

Objective: This multicenter study compared radiological parameters and clinical outcomes between surgical and nonsurgical management and investigated treatment characteristics associated with the successful management of unstable atlas fractures.Methods: We retrospectively evaluated 53 consecutive patients with unstable atlas fracture who underwent halo-vest immobilization (HVI) or surgical fixation. Clinical outcomes were assessed using neck visual analogue scale and disability index. The radiological assessment included total lateral mass displacement (LMD) and the anterior atlantodental interval (AADI).Results: Thirty-two patients underwent surgical fixation and 21 received HVI (mean follow-up, 24.9 months). In the surgical fixation, but not in the HVI, LMD, and AADI showed statistically significant improvements at the last follow-up. The osseous healing rate and time-to-healing were 100% and 14.3 weeks with surgical fixation, compared with 71.43% and 20.0 weeks with HVI, respectively. Patients treated with HVI showed poorer neck pain and neck disability outcomes than those who received surgical treatment. LMD showed an association with osseous healing outcomes in nonoperative management. Clinical outcomes and osseous healing showed no significant differences according to Dickman’s classification of transverse atlantal ligament injuries.Conclusion: Surgical internal fixation had a higher fusion rate, shorter fracture healing time, more favorable clinical outcomes, and a more significant reduction in LMD and AADI compared to nonoperative management. The pitfalls of external immobilization are inadequate maintenance and a lower probability of reducing fractured lateral masses. Stabilization by surgical reduction with interconnected fixation proved to be a more practical management strategy than nonoperative treatment for unstable atlas fractures.
Germination dynamics of sesame seeds under salt stress
Authors: Batista Silva Rodrigues Marilia , Rocha Limão Marcelo Augusto , Da Silva Santos Adriana , Da Silva Barbosa Luana , Pinheiro Lopes Kilson , Souto Ribeiro Wellington
Published: October 2022

Sesame crops have large economic potential; however, the scarcity of good quality water in the Semiarid region of the Northeast of Brazil can affect production systems, which requires the search for cultivars that can better be adapted to the local conditions. In this context, the objective of this work was to evaluate the germination dynamics of sesame seed lots subjected to different electrical conductivities of the imbibition solution. The experiment was conducted at the Laboratory of Seeds and Seedling Analysis of the Federal
Pengaruh Tingkat Kerapatan dan Kedewasaan Hutan Mangrove dalam Memerangkap Sedimen di Muara Sungai Langsa Kota Langsa
Authors: Erlangga Erlangga , Gusnita Helmi , Syahrial Syahrial , Akla Cut Meurah Nur , Imamshadiqin Imamshadiqin , Ezraneti Riri , Firdaus Rian
Published: October 2022

In November 2021, a study on the effect of mangrove density and maturity level on sediment trapping in the Langsa River estuary, Langsa City, was conducted with the objective of determining the density, maturity, and rate of sediment deposition around the mangrove forest, as well as the relationship between mangrove density and maturity level and sediment deposition rate. Mangrove vegetation was collected using a 40 m line transect parallel to the river flow and a 10 x 10 m plot, while sediment samples were collected using a sediment trap. The study's findings indicated that mangrove density varied between 675.00 and 1541.67 ind/ha, that mangrove maturity was uneven, with trunk diameters ranging between 4.14 and 9.83 cm and basal areas ranging between 30.45 and 72.93 m2/ha. Meanwhile, sediment deposition rates ranged between 26.94 and 277.73 mg/cm2/day, with a strong correlation between density and maturity of mangrove vegetation (98% and 99%, respectively).   Penelitian terhadap pengaruh tingkat kerapatan dan kedewasaan hutan mangrove dalam memerangkap sedimen di muara Sungai Langsa Kota Langsa dilakukan pada bulan November 2021 dengan tujuan untuk mengetahui tingkat kerapatan, kedewasaan dan laju pengendapan sedimen di sekitar hutan mangrovenya serta untuk mengetahui keterkaitan antara tingkat kerapatan dan kedewasaan hutan mangrove terhadap laju pengendapan sedimennya. Vegetasi mangrove dikumpulkan dengan menarik transek garis sepanjang 40 m yang sejajar dengan aliran sungai dan dibuat plot berukuran 10 x 10 m, sedangkan sampel sedimennya diambil menggunakan sediment trap. Hasil penelitian memperlihatkan bahwa kerapatan mangrovenya berkisar antara 675.00 – 1541.67 ind/ha, kedewasaan mangrovenya tidak merata dengan diameter batangnya berkisar antara 4.14 – 9.83 cm dan basal areanya berkisar antara 30.45 – 72.93 m2/ha. Sementara untuk laju pengendapan sedimennya berkisar antara 26.94 – 277.73 mg/cm2/hari, dimana keterkaitan antara kerapatan dan kedewasaan vegetasi mangrovenya memiliki hubungan yang kuat (masing-masing 98% dan 99%).
Komunitas Spons (Porifera) pada Ekosistem Terumbu Karang di Manokwari, Indonesia
Authors: Sayori Nelly , Tururaja Tresia S. , Kolibongso Duaitd
Published: October 2022

Sponges are one of the most influential benthic organisms in coral reef ecosystems. Many studies on sponge communities have been carried out globally, from the tropics to the sub-tropics. However, in Indonesia, the sponge community has not been sufficiently observed, especially its diversity and interactions with habitats. Manokwari, a developing city north of the Bird's Head Seascape region, Papua has a lack of information on benthic communities and no reports of sponges. This study is to examine the sponge community (diversity and distribution) in coral reef ecosystem. This study found that sponge richness (species and morphology) was categorized as “low”, with only 11 species with 8 morphological forms. The most common species included Niphates erecta, Stylissa carteri, and Pseudoceratina purpurea, while the most common growth forms were massive and encrusting, accounting respectively for 27.3% and 18.2% of the total number of species. The highest diversity was found on the island of Kaki (5 species) with the island of Nusmapi having an uneven distribution of sponges. The results of our study found that there was no relationship between sponge diversity and morphology. This baseline information is essential for management of marine biodiversity hotspots in taking decisions for marine life conservation.  Spons merupakan salah satu organisme bentik yang paling berpengaruh dalam ekosistem terumbu karang. Banyak penelitian tentang komunitas spons telah dilakukan secara global, dari daerah tropis hingga sub tropis. Namun di Indonesia, komunitas spons belum cukup diamati, terutama keanekaragaman dan interaksinya dengan habitat. Manokwari, kota berkembang di utara dari wilayah Bentang Laut Kepala Burung (BLKB), Papua memiliki kekurangan informasi tentang komunitas bentik dan tidak ada laporan tentang spons. Studi ini untuk mengkaji komunitas spons (keanekaragaman dan distribusi) pada ekosistem terumbu karang. Penelitian ini menemukan kekayaan spons (spesies dan morfologi) yang dikategorikan “rendah”, dengan hanya 11 spesies dengan 8 bentuk morfologi. Spesies yang paling umum termasuk Niphates erecta, Stylissa carteri, dan Pseudoceratina purpurea, sedangkan bentuk pertumbuhan yang paling umum adalah massif dan encrusting dengan menyumbang masing-masing 27,3% dan18,2% dari total jumlah spesies. Keanekaragaman tertinggi ditemukan di pulau Kaki (5 spesies) dengan pulau Nusmapi memiliki sebaran spons tidak merata. Hasil penelitian kami menemukan tidak ada hubungan antara keanekaragaman spons dengan bentuk morfologi. Informasi dasar ini sangat penting untuk pengelolaan hotspot keanekaragaman hayati dalam perumusan keputusan untuk konservasi biota laut.
Aktivitas Antijamur dari Bakteri Sedimen Mangrove Terhadap Candida albicans dan Malassezia furfur
Authors: Setyati Wilis Ari , Arifidyani Ananda , Susanto AB
Published: October 2022

Candida albicans and Malassezia furfur are fungal pathogens that can cause skin infections. This disease can be easily transmitted from one individual to another. The use of antibiotic drugs can cause pathogenic fungi to become resistant. Therefore, there is a need for the development of new antifungal substances, one of which comes from mangrove sediment bacteria. This research was conducted with the aim of obtaining bacterial isolats from mangrove sediments that have the ability to fight the fungal pathogens Candida albicans and Malassezia furfur. The method in this research is descriptive exploratory laboratory which includes isolation sampling and isolat purification, morphological characterization, screening, antifungal activity test and biochemical test. The results of this study were obtained 3 bacterial isolats that have antifungal activity against the fungal pathogens Candida albicans and Malassezia furfur. The results of the identification of biochemical tests showed that the isolat S.ISP2 was identified as the genus Pseudomonas; isolat S.ISP4 identified as Genus Moraxella; and isolat KJ.MA1 identified as Genus Vibrio  Candida albicans dan Malassezia furfur merupakan patogen jamur yang dapat menyebabkan penyakit infeksi kulit. Penyakit ini dapat dengan mudah ditularkan dari satu individu ke individu lainnya. Penggunaan obat antibiotik dapat mengakibatkan jamur patogen menjadi resisten. Oleh karena itum eprlunya ada pengembangan zat antijamur baru yang salah satunya berasal dari bakteri sedimen mangrove. Penelitian ini dilakukan dengan tujuan untuk mendapatkan bakteri isolat dari sedimen mangrove yang memiliki kemampuan untuk melawan jamur patogen Candida albicans dan Malassezia furfur. Metode pada penelitian ini adalah deskriptif eksploratif laboratory yang meliputi sampling isolasi dan purifikasi isolat, karakterisasi morfologi, skrining, uji aktivitas antijamur dan uji biokimia. Hasil dari penelitian ini adalah didapatkan 3 bakteri isolat yang memiliki aktivitas antijamur terhadap jamur patogen Candida albicans dan Malassezia furfur. Hasil identifikasi uji biokimia menunjukkan bahwa isolat S.ISP2 teridentifikasi sebagai Genus Pseudomonas; isolat S.ISP4 teridentifikasi sebagai Genus Moraxella; dan isolat KJ.MA1 teridentifikasi sebagai Genus Vibrio.
Alginate from Sargassum sp. Improve the Hematology Performance and Oxygen Tolerance Exposure of Lates calcarifer
Authors: Yudiati Ervia , Djunaedi Ali , Azhar Nuril , Alghazeer Rabia O.
Published: October 2022

Indonesia's fish production is abundant, especially in aquaculture. Lates calcarifer is farmed fish species. The failure of L. calcarifer cultivation due to disease problems. We are utilizing a natural compound derived from tropical Sargassum sp. extracts, namely alginate. This study aims to determine and analyse the supplementation of alginate in the diet by oral administration to improve the fish's hematological performance and oxygen stress tolerance. There were one control and three treatments (2 g.kg-1, 4 g.kg-1, and 8 g.kg-1 alginate/feed). The experiment was a completely randomized design with three replications. Fish were reared in a 350 L fiber tank for 12 days at a density of 20 ind.tank-1. Stress oxygen tolerance was applied by rearing the 10 fish in 12 L fully plastic-wrapped containers. The parameters test analyzed were phagocytic activity and phagocytic index, red blood parameters which are hematocrit and hemoglobin. The survival rate after 5 hr anoxic exposure was also recorded. The best treatments were achieved at a dose of 6 g.kg-1 and 8 g.kg-1in all parameters, except the phagocytic index. The alginate addition at all oxygen stress treatments also performed a better survival rate compared to the control. Adding alginate to feed as feed supplementation by oral administration can boost hematological performance and higher tolerance from oxygen stress exposure.
Effects of drought stress on growth and flavonoid accumulation of fish mint (Houttuynia cordata Thumb.)
Authors: Thanh Thang Tran , Ngan Thi Thanh Le
Published: October 2022

Fish mint (Houttuynia cordata Thumb.) is a popular medicinal plant grown primarily because of its pharmacological values. Drought stress has on the relationship between growth and physio-biochemical changes, especially flavonoid content. The impacts of various drought stress conditions on the fish mint development were investigated, including 85% of field capacity (FC), 75% FC, 65% FC and 55% FC in 14, 21 and 28 days. Agronomic, physiological and biochemical parameters during the growth of fish mint plants under drought stress conditions were assessed. According to the results of variance analysis, drought stress results in a considerable drop in the measured parameters (shoot height, leaf number, leaf area and fresh weight). Similarly, all of the above-mentioned parameters were also decreased with increasing the number of drought days. Furthermore, drought period and level caused a drop in respiration, photosynthetic rate, chlorophyll and starch content. The concentration of carotenoids and flavonoids, on the other hand, increased dramatically as drought stress periods and levels increased. In comparison to the control, the drought treatment (65% FC) in 7 days maintained the growth rate and increased flavonoid accumulation from 2.42 mg to 3.04 mg. These findings might give a scientific foundation for growing fish mint plants under drought stress to boost flavonoid content.
Desiccation induced physiological and biochemical changes of Gymnacranthera canarica (King.) Warb. seeds in the Myristica swamp forests, Southern Western Ghats, India
Authors: Anusha S , Anilkumar C , Gangaprasad A
Published: October 2022

Gymnacranthera canarica (King.) Warb. is an endemic tree species that dominates the Myristica swamp ecosystem of southern Western Ghats. This tropical tree species has become more threatened due to limited natural seed germination and habitat loss. Mature seeds were collected from the myristica swamp ecosystem subjected to desiccation study. This research evaluated the physiological (moisture content, tetrazolium reduction, lipid peroxidation, electrolyte leakage) and biochemical response of seeds during different desiccation treatments. Results showed that G. canarica seeds are highly sensitive to desiccation and total viability loss was seen within 15 days following harvest indicating the active seed metabolism of mature seeds showing absence of metabolic arrest. Desiccation enhanced malondialdehyde and electrolyte leakage while reducing formazan formation. Seed desiccation increases protease activity, which peaks when viability is lost. Desiccation reduced the quantity of phenol and starch, whereas proline, fat, sucrose and total soluble carbohydrates increased. The early viability loss in G. canarica seeds could be due to loss of membrane integrity, which was linked to ROS formation and associated lipid peroxidation products indicating seeds are truly recalcitrant.
Solving ill-structured problems mediated by online- discussion forums: Mass customisation of learning
Authors: Chandrasekaran Ramya , Tan Aik Ling , Tan Seng Chee , Yeong Foong May
Published: October 2022

To foster students’ learning of critical-thinking skills, we incorporated ill-structured problems in a Human Diseases module for third-year Life Sciences students. Using a problem-solving rubric and working in groups of three, students attempted to solve problems presented to them. We mediated their discussions by asynchronous online discussion forums (AODFs) as part of mass customisation of learning for 40 students where personalised learning was constrained by structure of the module. We examined the quality of students’ discussion, focusing on the feedback group members provided to one another, using an interpreted Structure of Observed Learning Outcomes (SOLO) taxonomy to code students’ feedback. Our analysis indicated that the students were able to provide uni-structural and multi-structural level in relation to solving an ill-structured problem, even though they are not used to solving ill-structured problems. This indicated that in a mid-size class, while personalised-learning is not always easy, it is possible to mass customise learning for students using common ill-structured problems in a class by mediating problem-solving using student discussions as feedback. However, more can be done to scaffold peer feedback on solving ill-structured problems so that the level of collaborative-learning can be improved in a mass customised model that approaches personalised learning.
Vision - A space for digital learning and exploring pedagogies: Virtual world education
Authors: Gregory Sue , Gregory Brent , Jacka Lisa , Lierse Sharon , McDonald Marcus , Ellis David , Hearns Merle , Sukunesan Suku , Irwin Pauletta , John Blooma , Butler Des , Zagami Jason , Gaukrodger Belma
Published: October 2022

The Australian and New Zealand Virtual Worlds Working Group (VWWG) was established in 2009. Members of the group have written papers for ASCILITE conferences since 2010. Each paper’s intention is to provide an overview on using virtual worlds in higher education, especially the practical aspects of incorporating 3D worlds to enhance student learning. This VWWG’s paper looks at a virtual world as a space for digital learning and exploratory pedagogy – meaning, that the authors perhaps do not perceive virtual worlds as ordinary. However, they do acknowledge that those who do not regularly engage in virtual worlds may see it as new and innovative, perhaps even untested ground, but still with a degree of unfamiliarity. A survey was sent to the members of the VWWG in which the narratives have provided rich data for in depth understanding. The themes focused in the survey were ‘disruptive thinking’, ‘emerging ideas’ and ‘lateral connections’, from the perspective of the respondents’ experiences in the implementation of virtual worlds in education. They discuss the idea of a virtual world used in education as a new treasure, or perhaps it has been in use for some time and now classified as traditional.
Exploring knowledge reuse in design for digital learning: tweaks, H5P, constructive templates and CASA
Authors: Jones David
Published: October 2022

Higher education is being challenged to improve the quality of learning and teaching while at the same time dealing with challenges such as reduced funding and increasing complexity. Design for learning has been proposed as one way to address this challenge, but a question remains around how to sustainably harness all the diverse knowledge required for effective design for digital learning. This paper proposes some initial design principles embodied in the idea of Context-Appropriate Scaffolding Assemblages (CASA) as one potential answer. These principles arose out of prior theory and work, contemporary digital learning practices and the early cycles of an Action Design Research process that developed two digital ensemble artefacts for 7 courses (units, subjects) and in less than a year been used in over 60 sites. Experience with this approach suggests it can successfully increase the level of design knowledge embedded in digital learning experiences, identify and address shortcomings with current practice, and have a positive impact on the quality of the learning environment.
Challenges in sustaining technology enhanced learning: Recruitment, employment and retention of learning designers in Australian universities
Authors: Slade Christine , McGrath Dominic , Greenaway Ruth , Parker Jeffrey
Published: October 2022

Universities require learning designers with creative digital experience and significant knowledge of educational theory and practice to meet institutional teaching and learning strategic priorities. Little is known about how best to attract and retain learning designers or alternatively, to assist learning designers navigate their career pathways in higher education. In response to these needs, this study aimed to provide a snapshot of current learning design practices across Australian universities; to document relevant skills, knowledge, education and professional background, role types and employment conditions, challenges for directors, and to identify areas for future attention. The researchers used two separate online surveys to target 1) directors of central teaching and learning units and 2) learning designers employed in universities. The results are useful as a means to sustain and improve practice through evidence-based decision-making in the conceptualisation of learning designer positions, the management and retention of learning design staff, and to assist learning designers in their career choices.
Designing with Constraints: Are we designing for creativity or compliance?
Authors: Dave Kashmira , Gregory Sue
Published: October 2022

Presented is a concept that forms the basis of a larger research project aiming to understand the university lecturers design practices around bureaucratic constraints and, as a result, if they were still able to be creative. This idea was developed from the research findings of one of the author’s PhD dissertation (Dave, 2017) wherein the lecturers expressed their design practice impacted by the various constraints which are explained in this paper. The authors argue that like other professional designers (engineers, architects), lecturers should be able to be creative despite the constraints. Also raised are the higher-level issue of learning and training in the area of higher education which makes people more responsive to creativity.
The potential of Augmented Reality to amplify learning and achieve high performance in the flow of work
Authors: Steel Caroline
Published: October 2022

The longevity of work skills and knowledge is constantly diminishing. This phenomenon provokes significant challenges for both employees and industries. There is a growing realization that we must all continue to ‘learn to earn’ (Bersin, 2015) in order to maintain employability to sustain the economic means to live life. This short conceptual paper proposes that there are increasing opportunities to learn new capabilities and practices and achieve high performance through learning in the flow of work. It explores the potential and constraints of Augmented Reality, as an emerging technology, to amplify workplace learning.
Conversational Bots as Electronic Performance Support System for the Professional Development of Teacher Educators
Authors: Thilarajah Shamini , Divaharan Shanti
Published: October 2022

With the increased demand and interest in designing technology-enhanced lessons for 21st Century learners, ways to empower and enable teacher educators to use educational technologies effectively underpinning sound instructional strategies had to be reinvented. Teacher educators may require on-demand support in order to have the necessary knowledge and skills to meet the needs of the rapidly changing learning landscape. A learning intervention or a job aid had to be developed to provide just-in-time bite-sized knowledge in time of need. This conceptual paper elaborates the design and development of a next generation electronic performance support system for teacher educators and outlines a possible approach of using a rules-based chatbot as a means of empowering teacher educators to become active designers of meaningful technology-enhanced lessons.
Normalising practice – moving a technological implementation from project phase to operational phase
Authors: Trahar Priscilla , Bourke John
Published: October 2022

It is widely acknowledged that successful implementation of new technologies relies on a project-based institutional approach with a dedicated and motivated project team to champion the technology and support professional development. One challenge that arises following project-style implementations is how to transition support structures and project drivers to 'normalisation' phase where technologies are seen as 'business-as-usual'. Griffith University’s PebblePad implementation project team deliberately planned an end-of-project to business-as- usual transition. This planning involved; embedding PebblePad in the institution’s Virtual Learning Environment (VLE); setting up ongoing scalable support structures; and ensuing there were suitable institutional partnerships to sustain the diffusion of the institutional adoption. Griffith’s implementation project was led by the central learning & teaching unit (Learning Futures) and relied heavily on buy-in and support from many institutional stakeholders. Therefore it was essential that stakeholder teams were empowered to maintain the existing users and continue to look for opportunities for targeted and meaningful implementation. Similarly, Learning Futures had to find staffing capacity to continue to support PebblePad as a technology within the VLE. This ongoing work involves maintaining vendor relationships, providing ongoing professional development and continuing to be a centralised point for managing internal and external communications between stakeholders.
The influence of proteoforms: assessing the accuracy of total vitamin D-binding protein quantification by proteolysis and LC-MS/MS
Authors: Kilpatrick Lisa E. , Bouillon Roger , Davis W. Clay , Henderson Clark M. , Hoofnagle Andrew N. , Pauwels Steven , Vanderschueren Dirk , Waelkens Etienne , Wildiers Hans , Yen James H. , Phinney Karen W.
Published: October 2022

Objectives

Vitamin D-binding protein (VDBP), a serum transport protein for 25-hydroxyvitamin D [25(OH)D], has three common proteoforms which have co-localized amino acid variations and glycosylation. A monoclonal immunoassay was found to differentially detect VDBP proteoforms and methods using liquid chromatography-tandem mass spectrometry (LC-MS/MS) might be able to overcome this limitation. Previously developed multiple reaction monitoring LC-MS/MS methods for total VDBP quantification represent an opportunity to probe the potential effects of proteoforms on proteolysis, instrument response and quantification accuracy.

Methods

VDBP was purified from homozygous human donors and quantified using proteolysis or acid hydrolysis and LC-MS/MS. An interlaboratory comparison was performed using pooled human plasma [Standard Reference Material® 1950 (SRM 1950) Metabolites in Frozen Human Plasma] and analyses with different LC-MS/MS methods in two laboratories.

Results

Several shared peptides from purified proteoforms were found to give reproducible concentrations [≤2.7% coefficient of variation (CV)] and linear instrument responses (R2≥0.9971) when added to human serum. Total VDBP concentrations from proteolysis or amino acid analysis (AAA) of purified proteoforms had ≤1.92% CV. SRM 1950, containing multiple proteoforms, quantified in two laboratories resulted in total VDBP concentrations with 7.05% CV.

Conclusions

VDBP proteoforms were not found to cause bias during quantification by LC-MS/MS, thus demonstrating that a family of proteins can be accurately quantified using shared peptides. A reference value was assigned for total VDBP in SRM 1950, which may be used to standardize methods and improve the accuracy of VDBP quantification in research and clinical samples.
The new, race-free, Chronic Kidney Disease Epidemiology Consortium (CKD-EPI) equation to estimate glomerular filtration rate: is it applicable in Europe? A position statement by the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)
Authors: Delanaye Pierre , Schaeffner Elke , Cozzolino Mario , Langlois Michel , Plebani Mario , Ozben Tomris , Cavalier Etienne
Published: October 2022

The EFLM recommends not to implement the race-free Chronic Kidney Disease Epidemiology Consortium (CKD-EPI) equation in European laboratories and to keep the 2009 version of the CKD-EPI equation, without applying a race correction factor. This recommendation is completely in line with a recent Editorial published by the European Renal Association who has also proposed to change to a novel equation only when it has considerably better performance, trying to reach global consensus before implementing such a new glomerular filtration rate (GFR) estimation equation. In Europe, this equation could be for instance the new European Kidney Function Consortium (EKFC) equation, which is population-specific, developed from European cohorts and accurate from infants to the older old. Beyond serum creatinine, the estimating equations based on cystatin C will probably gain in popularity, especially because cystatin C seems independent of race. Finally, we must keep in mind that all GFR equations remain an estimation of GFR, especially rough at the individual level. Measuring GFR with a reference method, such as iohexol clearance, remains indicated in specific patients and/or specific situations, and here also, the role of the clinical laboratories is central and should still evolve positively in the future.
The 2022 monkeypox outbreak in nonendemic countries: a review for the emergency department clinician
Authors: Cho Sun Young
Published: October 2022

Since May 2022, monkeypox (MPX) cases have been reported from several European countries, and this outbreak rapidly spread globally. Although MPX is not a new disease, most clinicians in nonendemic countries are unfamiliar with it. In addition, this current outbreak, unlike previous outbreaks in Africa, shows unique features in terms of epidemiology, transmission routes, and clinical manifestation. Most cases were men who have sex with men, had no travel history to an MPX endemic area, and presented with anogenital lesions, suggesting human-to-human transmission via close contact during sexual activity. In the emergency department setting, rapid identification of suspected cases and implementation of effective infection control and preventive measures are critical for preventing further transmission to healthcare workers and other patients. Emergency department clinicians should be aware of the clinical presentations of MPX and be alert to patients presenting with fever and vesicular rash or sexually transmitted disease-associated rash, especially among those with travel history to countries reporting an MPX outbreak. This brief review provides current information of MPX to help emergency department clinicians understand the epidemiology, transmission, clinical manifestation, diagnosis, treatment, and infection prevention and control of MPX.