bluuebunny/crossref_metadata_2025 huggingface dataset:
First report on optimization of loop-mediated isothermal amplification (LAMP) for the diagnosis of Babesia felis
Authors: Salim Muhammad Awais , Akhtar Raheela , Lateef Muhammad , Rashid Imran , Akbar Harron , Shehzad Wasim , Akhtar Fareeha , Islam Saher , Farooqi Shahid Hussain , Durranti Uzma Farid
Published: December 2017

The objective of present study was to optimize loop mediated isothermal amplification (LAMP) assay for the diagnosis of Babesia felis in cats. LAMP primers were designed recognizing four sections of 18SribosomalRNA (18S rRNA) gene of B. felis. The blood samples of cats microscopically positive for Babesia felis were further used to extract deoxyribo neuclic acid (DNA) and the reaction mixture of 25 µL was standardized at 63°C temperature for 1 hour. LAMP assay provided more positive samples than conventional polymerase chain reaction (PCR). The prevalence of B. felis was also determined in cats using this optimized LAMP assay and it was found that the prevalence was more in younger cats as compare to adults. The application of LAMP can be helpful in rapid, reliable and cost effective diagnosis of B. felis in field.
Investigation of bacterial and fungal agents from cutaneous lesions in canine Leishmaniasis
Authors: Parin Ugur , Erbas Goksel , Savasan Serap , Ural Kerem , Yuksel Hafize Tugba , Balat Gamze , Kirkan Þukru
Published: December 2017

The objective of the present study was to determine the bacterial and fungal agents accompanying with cutaneous lesions of dogs suffering from Canine Visceral Leishmaniasis (CVL). Sterile swap samples were taken from cutaneous lesions inspected of ears, dorsal, collar, pectoral, inguinal and interdigital spaces of 20 dogs, in which diagnosis were based on a combination of clinical symptoms, anti-leishmania antibody titers by use of Immunofluorescence antibody test (IFAT), and dogs were further classified according to the Leishvet Guidelines involving serological, hematological, serum biochemical, and urinalysis. Swap samples were inoculated to culture media for isolation and identification of bacterial and mycotic agents. 48 Staphylococcus aureus, 4 Bacillus cereus, 8 Staphylococcus epidermidis, 24 Candida albicans, 24 Microsporium canis and 4 Aspergillus niger were isolated and identified in both group of dogs. Regarding antibiotic susceptibility test, the isolates were found 100% susceptible to cefoperazone and amoxicillin-clavulanic acid, 53% to danofloxacin, 46% of the isolates were susceptible to enrofloxacin, and all of the isolates were resistant (100%) to penicillin G and gentamicin. In conclusion, the antibacterial and antifungal therapy should be provided in accordance with antibiotic susceptibility tests in skin lesions of dogs suffering with CVL.
Effect of Microencapsulated Butyric Acid supplementation on growth performance, ileal digestibility of protein, gut health and immunity in Broilers
Authors: Imran M. , Ahmed S. , Ditta Y. A. , Mehmood S. , Rasool Z. and M.W. Zia , Zia M. W.
Published: December 2017

The effect of microencapsulated butyric acid (MEBA) on growth performance, ileal digestibility (ID) of protein, gut health and immunity was assessed in broilers. A total of 336 d-old Hubbard Classic broilers chicks were randomly assigned to four dietary treatments (Control, 0.25, 0.35 and 0.45 g/kg of MEBA). Each treatment was replicated three times with 28 birds/replicate. Feed intake, body weight gain, FCR, intestinal morphology, ID of protein and immunity was evaluated. At 35-d of age, three birds/replicate were randomly selected and slaughtered to collect blood, duodenal samples, and ileal digesta. The Improved body weight gain (P = 0.05), FCR (P less than 0.01), duodenal villous height (P less than 0.05) and ID (P less than 0.1) were observed; whereas, unresponsive treatment effect with respect to feed intake (P>0.5) and antibody titer against Newcastle disease (P>0.05) were noted. The results indicated that MEBA improves digestion and absorptive processes which consequently improved the broilers performance.
Effect of different bedding materials on growth performance, physiological response and economic efficiency in three commercial broiler strains
Authors: Irfan M. , Mehmood S. , Hussain J. , Saima . , Shaheen M. S. , Ahmad S. , Zia M. W.
Published: December 2017

The experiment was conducted at Ravi Campus of the University of Veterinary and Animal Sciences, Lahore, Pakistan to assess the effect of wood-shaving, wheat-straw, corn-cob pulp and rice-husk (bedding materials) on the performance of three commercial strains of broiler viz, Ross-308, Cobb-500 and Arbor Acres. Three-hundred-sixty day-old chicks, 120/ strain were distributed into 36 replicates (10 birds/ replicate) according to 4×3 factorial arrangements. Weekly weight gain, FCR, livability and rearing cost was recorded. Birds raised on corn cob pulp exhibited enhanced (P£0.05) body weight, FCR, lesser rearing cost and superior (P£0.05) livability. Among strains, Ross-308 and Cobb-500 showed improved performance compared to Arbor Acres. Serum glucose and immune response were not affected by bedding material or strain; serum cholesterol was higher at rice husk; abdominal fat pad and foot pad dermatitis were higher at corn cob pulp and wood shaving. It was concluded that corn cob pulp proved to be a better bedding material, especially in Ross-308 and Cobb-500.
Despertando a formação inteligente: transformando o prazer da leitura em conhecimento integral a crianças, adolescentes e jovens
Authors: Michelotti Patricia
Published: December 2017

A literatura, sabidamente, é importante instrumento de formação e aprendizado, mas também pode ter sua função amplificada no tocante à descoberta de gostos e preferências. O presente trabalho busca esclarecer como a atuação do projeto Despertando a Formação Inteligente por meio da Leitura proporciona aos participantes a possibilidade de descoberta de si. Para isso descreve a atuação do projeto e sua forma de ação, bem como os resultados que já foram alcançados. O projeto proporcionou, ao longo de seus meses iniciais, uma reflexão individual tanto por parte dos ministrastes das oficinas quanto dos participantes. Sendo assim, entende-se que deve ter continuidade, expandindo sua atuação para cada vez mais alunos da AMF e de escolas da região.
A superação da desigualdade como forma de empoderar indivíduos: o caso dos alunos da escola Leonor Pires de Macedo em Restinga Sêca/RS
Authors: Santos Elaine dos
Published: December 2017

A atuação de um projeto de produção textual deflagrou a percepção da carência identitária dos alunos envolvidos. Assim sendo, optou-se pela realização de atividades que pudessem fazê-los (re) conhecer-se como membros de uma família, de uma escola, de uma comunidade.  Para tal, foram realizadas visitas e entrevistas com autoridades locais para que os alunos pudessem entender, por exemplo, o funcionamento de alguns setores da Prefeitura Municipal. Considera-se que essas atividades, sempre pautadas pelos relatos orais ou escritos dos alunos, tenham servido para que eles alcançassem uma nova percepção do mundo em que se inserem, fazendo-se autônomos e capazes de intervir em demandas sociais.
A aplicação da Pedagogia Ontopsicológica no desenvolvimento profissional dos jovens
Authors: Gehrke Augusto Roberto , Schaefer Ricardo
Published: December 2017

Diante da crise da juventude evidenciada na sociedade contemporânea, novas alternativas pedagógicas estão sendo criadas e testadas a fim de restituir aos jovens a possibilidade da autoconstrução responsável enquanto pessoas e profissionais. Buscando analisar e descrever iniciativas já existentes nessa direção, este artigo investiga os resultados da aplicação da pedagogia ontopsicológica nos alunos da Faculdade Antonio Meneghetti que moram na Casa do Estudante, a partir de uma pesquisa empírica que teve como enfoque a dimensão do trabalho e o desenvolvimento profissional dos jovens.  Para atingir esse objetivo, adotou-se um questionário com dados qualitativos e quantitativos em 40 alunos que moram na Casa do Estudante situada no Centro Internacional de Arte e Cultura Humanista Recanto Maestro. Os resultados evidenciam que, por meio do trabalho, os jovens desenvolvem uma competência competitiva e habilidades relacionadas à liderança, criando hábitos pessoais e profissionais funcionais ao próprio crescimento e desenvolvendo o protagonismo responsável. A aplicação da pedagogia ontopsicológica contribui para a formação integral do ser humano e pode ser uma real alternativa à crise da juventude que evidenciamos na sociedade contemporânea.
Aplicação da Pedagogia Ontopsicológica nos Projetos da Fundação Antonio Meneghetti: uma análise de conteúdo
Authors: Ribeiro Eloisa Vieira
Published: December 2017

A Fundação Antonio Meneghetti tem mais de 25 Projetos ativos, que envolvem mais de mil crianças, adolescentes e adultos, sendo Projetos educacionais, culturais, de incentivo à pesquisa, e têm como principal objetivo o desenvolvimento do ser humano. Tais projetos têm como base a Pedagogia Ontopsicológica e sua aplicação nas mais diversas áreas. Para que esses projetos continuem crescendo, ajudem no desenvolvimento de crianças e adolescentes, foi desenvolvida essa pesquisa, que verificou os resultados que a Fundação Antonio Meneghetti e os participantes dos Projetos vem tendo.
DISFEMISME DALAM PERSPEKTIF SEMANTIK, SOSIOLINGUISTIK, DAN ANALISIS WACANA
Authors: Laili Elisa Nurul
Published: December 2017

Dysphemism is an expression with connotations that are offensive either about the denotatum or the audience. It is used to talk about one’s opponents, things one wishes to show disapproval of, and things one wishes to be seen to downgrade. The topic of dysphemism is crucial to investigate because dysphemism is the infraction of the rules of politeness that deals with the matters of face and face effects.This research uses descriptive qualitative method. The approach used in this research is library research. Data taken from taking notes and observation.This article is written to explain dysphemism in the perspective of Semantics, Sociolinguistics, and Discourse Analysis.
KEPRIBADIAN TOKOH DALAM NOVEL MENCARI PEREMPUAN YANG HILANG (KAJIAN PSIKOANALISIS CARL GUSTAV JUNG)
Authors: Septiarini Tri , Sembiring Renni Handayani
Published: December 2017

Dewasa ini, psikoanalisis yang lazim digunakan sebagai pendekatan penelitian adalah aspek kaidah-kaidah psikologi yang terdapat dalam karya sastra khususnya karakter tokoh-tokoh utama. Novel Mencari Perempuan yang Hilang karya DR. Imad Zaki, alih bahasa oleh Dr. Zuriyati menceritakan kisah pilu seorang wanita yang menghilang dari peradaban karena berat untuk menanggung beban kejahatan ayahnya yang merugikan orang banyak. Penelitian ini bertujuan untuk: (a) mengetahui bagaimana deskripsi tokoh-tokoh utama dalam novel yaitu Dokter Ahlam, Dokter Sholeh dan Abdul Ghani Zahabi; (b) mengetahui bagaimana tingkat kepribadian pada masing-masing tokoh utama; dan (c) mengetahui tipe kepribadian tokoh-tokoh utama dalam novel. Penelitian ini menerapkan analisis isi dengan  pendekatan kualitatif. Dokter Sholeh dan dokter Ahlam mempunyai karakter yang positif. Abdul Ghani Zahabi mempunyai karakter kejam. Dokter Sholeh cenderung memiliki tingkatan kepribadian kesadaran dan ketidaksadaran kolektif (persona). Dokter Ahlam cenderung memiliki tingkatan kepribadian kesadaran dan ketidaksadaran kolektif (animus). Dan Abdul Ghani Zahabi cenderung memiliki tingkatan kepribadian ketidaksadaran personal dan ketidaksadaran kolektif (persona, bayangan, dan anima). Dokter Sholeh cenderung termasuk kedalam tipe kepribadian ekstroversi (pemikir, perasa dan intuitif). Dokter Ahlam cenderung termasuk pada tipe kepribadian introversi (perasa dan intuitif). Dan Abdul Ghani Zahabi cenderung termasuk kedalam tipe kepribadian ekstroversi (pemikir dan perasa) dan introversi (pemikir dan intuitif).
Ankyloglossia (Tongue-Tie)—To Snip or Not to Snip: An Integrative Literature Review
Authors: Levkovich Ryndell , Sidebotham Mary , Vaughan Karolyn , Dietsch Elaine
Published: December 2017

PURPOSE: Ankyloglossia (commonly referred to as tongue-tie), is increasingly being reported as a significant contributory factor to difficulties experienced in establishing the mother–infant breastfeeding relationship. Frenotomy as a management option is contributing to international interest and interdisciplinary controversy. The aim of this article was to identify and examine the evidence in relation to frenotomy to inform practice for newborns diagnosed with symptomatic ankyloglossia.

DESIGN: An integrated literature review, informed by Whittemore and Knafl (2005) was used to identify and evaluate contemporary evidence.

MAJOR FINDINGS: There is a marked difference in opinion between health disciplines regarding frenotomy as a treatment option for ankyloglossia. There is agreement that support from a trained professional such as a lactation consultant prior to undergoing any invasive procedures to treat ankyloglossia is essential. Controversy persists however as to whether the condition will resolve spontaneously without treatment.

CONCLUSION: Although frenotomy does seem to resolve breastfeeding difficulties, little is known regarding long-term consequences for the baby and optimal timing for the procedure. This review indicates a need for education of professionals and standardization of assessment processes along with ongoing research to identify efficacy and optimal timing of frenotomy when used.
The Relationship of Anxiety Levels with Frequency of Dispneu Exacerbation in Asthma Bronchial's Patients at SMF Pulmo, Soebandi General Hospital, Jember
Authors: Hostiadi Michael , Mardijana Alif , Nurtjahja Edi
Published: December 2017

Asthma is a chronic inflamation of respiratory tract which cause bronchial hypereactivity to some triggers with dispneu as the main symptomp. Stress and emotional disturbance like anxiety can be the trigger of asthma attack. This research is to find out the relationship between anxiety level and the frequency of dispneu in asthma bronchial patients at SMF Pulmo, dr. Soebandi General Hospital, Jember. This research used a cross sectional observational analytical method and HARS questionare. Based on our observation to 30 patients, Chi square analytical test showed 0,000 significancy level with p < 0,05. It means that there is a relationship of anxiety levels with the frequency of dispneu in asthma bronchial patients at SMF Pulmo, dr.Soebandi General Hospital, Jember. Keywords: anxiety, dispneu, asthma bronchial
Antimicrobial in vitro Study of Cosmos caudatus H.B.K Leaves Extract Towards Methicillin-Resistant Staphylococcus aureus (MRSA)
Authors: Febianti Zahrah
Published: December 2017

Cosmos caudatus H.B.K leaves is known to have many active substances with antimicrobial effect such as saponin, flavonoid, poliphenol, volatile oil, costunolide, and 4,4’-bipyridin. This research is conducted to prove the antimicrobial effect of Cosmos caudatus leaves extract on gram positive Methicillin-Resistant Staphylococcus aureus (MRSA) in vitro. The sample of this research is served by Microbiology Laboratorium of Brawijaya University. An experimental study with tube dilution method was carried out. The treated groups are groups of bacteria treated with Cosmos caudatus leaves extract with a range concentrations of 14%; 12%; 10%; 8%; and 6%. The control group is groups treated with 0% extract. The result indicates that the MIC (Minimal Inhibitory Concentration) can not be observed due to the extract’s turbid green colour. The MBC (Minimal Bactericidal Concentration) is 14% for MRSA. Data analysis with CI=95% shows a significant difference of effects on the number of MRSA colonies growth (Anova, p = 0,000) with the change of extract concentrations. The correlation regression test shows a strong association between the extract concentration and the number of colonies growth (Correlation, r = -0,754, p=0,000). Cosmos caudatus leaves extract has antimicrobial effect on MRSA.Keywords : Cosmos caudatus leaves estract, Methicillin-Resistant Staphylococcus aureus (MRSA), antimicrobial.
Bioactivity of a Compound of Standardized Bangle (Zingiber cassumunar Roxb.) Extract Fraction as a Complimentary Therapy to Prevent Malaria Complications
Authors: Hermansyah Bagus , Utami Wiwien Sugih
Published: December 2017

Malaria is still a major problem in the international and national scale. World Health Organization (WHO) states that based on World malaria report, world cases of malaria reached 216 million and an estimated of 655 thousand people died, in 2010. Overproduction of pro-inflammatory cytokines by phagocytic cells and activated endothelial cells occurs In the severe malaria. Bangle (Zingiber cassumunar Roxb.) has the potential to be developed as a new complementary therapy that is expected to prevent fatal malaria complications. The research objective is to test in vivo, the activity of a compound of standardized Bangle (Zingiber cassumunar Roxb.) fraction extract as a complementary therapy along with standard anti-malarial drug, artemisin. This is a true experimental study using twenty-five mice Balb / C male which are divided into five groups; the negative control group, positive control group treated KI, II, and III stimulation with each of the compounds of standardized fraction extract of bangle ( FEBT-h, FEBT-dcm, FEBT-m) for 14 days. Oneway ANOVA test showed the result of p = 0.01 (p <0.05). Continued with LSD test that showed the most significant in lowering the degree of parasitaemia is the group of methanol (FEBT-m).Keywords: Zingiber cassumunar Roxb, fractionated extracts, complementary therapies, malaria
Effect of Mung Bean Sprout (Vigna radiata (L)) Extract on Physical Stress-Induced Atherosclerosis of Male Wistar Rat
Authors: Purwandhono Azham , Normasari Rena
Published: December 2017

Coronary heart disease is an important health problem. It is estimated that the death rate from coronary heart disease by 23,4 millions in 20130 (WHO, 2009). Excessive physical activity can cause oxidative stress which lead to atherosclerosis. Mung bean sprouts (Vigna radiata (L)) contain vitamin E, vitamin C, fenol, flavonoid, fitosterol, and other minerals. The aim of this study is to determine the effect of mung beans sprout extract on on the thicknes of the tunica intima-media on physical stress-induced atherosclerosis of male wistar rat. This research is true experimental study using 20 male wistar rat were divided into five groups: negative control, positive control and treatment groups P1, P2, P3 (physical stress + bean sprout extract 50 mg/day, 100 mg/day, 200 mg/day for 10 days). There is significant decrease of MDA serum level in the treatment groups.Keywords : Vigna radiata, physical stress, thicknes of tunica intima-media
Modulation of Antibiotic Activity Against Streptococcus pneumoniae by N-acetylcysteine and ascorbic acid
Authors: Agustina Dini , Kurniawan Bagus Dwi , Palupi Intan
Published: December 2017

Streptococcus pneumoniae is one of the bacterial pathogens that cause pneumonia. Pneumonia is the biggest cause of infant mortality in Indonesia around 23%. The main therapy pneumonia is antibiotics, but in some cases combined with ascorbic acid or N-acetylcysteine, which serves as a complementary therapy. The purpose of this study was to determine the effect of adding ascorbic acid or N-acetylcysteine ​​on antibiotics towards the growth of S. pneumoniae in vitro. The method used in this research is the method of germ-disc diffusion susceptibility test with S. pneumoniae growth inhibition is indicated by the formation of inhibition zone. Positive controls used clindamycin and erythromycin. The average diameter of inhibition zone on the negative control, positive control, and treatment of 1 to 5 on the addition of clindamycin with ascorbic acid are respectively 5; 24.12; 24.62; 26.08; 27.58; 28.74; and 29.76 mm. The data has a strong correlation to the Pearson test (R=+0,910). On a logarithmic regression analysis found minimal concentration of ascorbic acid can inhibit is 1.767 mg/ml. While the addition of erythromycin with N-acetylcysteine ​​are respectively 28.02; 25.62; 23.46; 22.42; and 21.68 mm. Pearson correlation test results of these data are the opposite relationship between the diameter of inhibition zone and the concentration of N-acetylcysteine ​​with a very strong correlation coefficient. Logarithmic regression of the minimum concentration of N-acetylcysteine ​​which may decrease the antibacterial activity of erythromycin inhibit the growth of Sreptococcus pneumoniae amounted 1,66mg/ml. It is concluded that ascorbic acid increases the activity of clindamycin in inhibiting the growth of S. pneumoniae in vitro and N-acetylcysteine ​​may decrease the antibacterial activity of erythromycin in inhibiting the growth of S. pneumoniae in vitro. Keywords: S. Pneumoniae, ascorbic acid, N-asetilsitein, inhibition zone
Correlation of the Mastoid Pneumatization and Bone Destruction in CSOM with Acquired Cholesteatoma using Computed Tomography
Authors: Nilasari Dyah , Ningrum Farah H , Naftali Zulfikar
Published: December 2017

Chronic Suppurative Otitis Media (CSOM) is still a public health problem especially in developing countries. CSOM with acquired cholesteatoma accompanied referred to as the type of hazard, as they often lead to dangerous complications and have a high rate of morbidity and mortality. Mastoid CT with 0,625mm slice thicness may reveal detail cholesteatoma and bone destruction. It’s an analytic observational study with cross sectional design. This study consisted of 30 cases CSOM, aged 18-60 years and had a 0,625mm slice thicness CT scan mastoid examinations in Dr. Kariadi Hospital Semarang and indicating a cholesteatoma and bone destructions. The result showed that hypopneumatization type and mild degree of bone destructions obtained in most subjects, each 20 and 21, but there’s no correlation between mastoid pneumatization and bone destruction (p 0,367 or p > 0,05). This study showed more lower type of the mastoid pneumatization, more severe degree of bone destructions.Keywords: CSOM, mastoid pneumatization, Mastoid CT
The Correlation Between Carbohydrate Consumption with Blood Pressure Levels of Elderly Communities in Sumbersari Jember
Authors: Cinintya Revin Fiona , Rachmawati Dwita Aryadina , Hermansyah Yuli
Published: December 2017

The increasing population of elderly causes getting more complex illness because impairment in organ function, one of them is the blood vessels. Blood vessels will have decreased nitrite oxide that causes atherosclerosis, as the primary cause of the hypertension. The change of lifestyle and nutrient factor is related to the incident of hypertension. Meanwhile, Indonesians tend to prefer food sources of carbohydrate. The purpose of this research is to analyze relations between consumption of carbohydrate with blood pressure on elderly from active members of Karang Wreda Sriwijaya and Semeru Jaya Sumbersari Jember about 47 samples. This research is an analytic observational research with the cross sectional methods. In this research, samples were interviewed to describe the charracteristic ( the age, sex, the history of hypertension). Then samples were interviewed to know their carbohydrate consumption using 24-hours Food Recall method three times in different time with the maximum of two weeks and the blood pressure was checked with a mercurial sphygmomanometer. The results of this research based on the correlation of Spearman obtained a value of p=0,00 for the number of subject’s carbohydrates consumption with systolic blood pressure and p=0,02 for diastolic pressure. The conclusion is there is a correlation between carbohydrates consumption with systolic and diastolic blood pressure.Keywords: carbohydrates consumption, blood pressure, elderly
Difference in The Incidence Severe Preeclampsia and Eclampsia between Maternal Age 20-34 Years and >34 Years in dr. Soebandi Hospital Jember
Authors: Ali Muhammad Fakhri , Hadisubroto Yonas , Firdaus Jauhar
Published: December 2017

Maternal Mortality Rate (MMR) in Indonesia is still high. The maternal mortality rate continues to rise due to hypertension, one of which is caused by pre-eclampsia and eclampsia. Many factors cause preeclampsia, including advanced maternal age. The purpose of this study was to determine the effect of advanced maternal age during pregnancy with severe preeclampsia and eclampsia in RSD dr. Soebandi Jember. This study used cross sectional approach using 264 samples were divided into two groups, there are pregnant women aged 20-34 years and >34 years. The results of data analysis using Chi Square for severe preeclampsia and obtained p = 0.015 and OR = 2.494, which means there is a significant difference in comparison severe preeclampsia between gestational age of 20-34 years and >34 years. At the age of 20-34 years from 216 samples found 28 people suffering from severe preeclampsia (12.9%). Whereas at the age of mother> 34 years of 48 people found 13 people (27.1%) suffered severe preeclampsia Results of data analysis obtained eclampsia using Fisher and p = 0.554, which means there are no significant differences in comparison eclampsia between gestational age of 20-34 years and >34 years. At the age of 20-34 years from 216 samples found 3 people suffering from eclampsia (1.38%). While at mother age> 34 years from 48 people found 1 person (2.08%) suffered eclampsia.
Immunoblotting Detection of Immunoglobulin G Post Subcutaneous Immunization Of Protein Hemaglutinin Pili Klebsiella pneumoniae 12,8 kDa on Mice BALB / C
Authors: Agustina Dini
Published: December 2017

Klebsiella pneumoniae is the second most common cause of community infection and nosocomial infections due to Gram-negative bacteria. These bacteria are able to induce the onset of immune response, especially humoral immune response.Humoral immunity acts through the activation of B cells that produce antibodies. Antibodies, especially IgG, will cause encapsulated bacteria such as Klebsiella pneumoniae to be better phagocytosed. The purpose of this study was to determine the IgG response to hemagglutinin protein pili K. pneumoniae 12.8 kDa. The method used in this research is Immunoblotting method with western blot and dot blot. The primary antibodies used for the western blot and dot blot tests were isolated from BALB / C mice serum induced with the subcutaneous pili K. pneumoniae 12.8 kDa protein. To get the standard in assessing the results of dot blot were used Corel Photo-paint X6. The semi-quantitative result of dot blot was obtained with the strongest reaction of the antibody dilution at 1/100 while the antigen dilution titer at 1 / 10.000. Results from western blotting showed a positive reaction of the pili protein subunit with a molecular weight of 128.1 kDa, 114.4 kDa, 64.9 kDa, 31.1 kDa, 27.7 kDa, 24.8 kDa, 20.9 kDa, 12.8 kDa, and 10 kDa. The conclusion of this study is the immunization of hemagglutinin pili K. pneumoniae 12.8 kDa subcutaneously capable of inducing the formationof Immunoglobulin G in BALB / C mice.
The Effect of Eccentric Activity on Glucose Transporter Type 4 in Gastrocnemius Muscle of Streptozotocin-induced Diabetes Mellitus Mice
Authors: Handoko Adelia , Purwanto Bambang , Mustika Arifa
Published: December 2017

Diabetes mellitus (DM) is a metabolic disorder with increasing mortality rate. Decrease in GLUT-4 levels was found in diabetics resulting in an increase in postprandial blood glucose levels. Various innovative treatment have been developed, one of them is exercise. Exercise is growing fast today, especially eccentric contraction based exercise that potentially increase GLUT-4 muscle’s level. The aim of the present study was to investigate the effect of eccentric activity on glucose transporter type 4 in gastrocnemius muscle of streptozotocin-induced diabetes mellitus mice. Diabetic mice were grouped randomly into 2 groups. One group was given single bout of eccentric excercise by downhill running on 10o degree decline treadmill and the other group was given eccentric activity by downhill running on 0o degree. The GLUT-4 muscle’s level were measured after treadmill. Data obtained from each group were then analyzed using independent t-test, significant results were found (p<0.05). The conclusion of this study is single bout eccentric downhill running activity with 10o angel increases the levels of GLUT-4 muscles.

Key words: Glucose Transporter Type 4, eccentric, Diabetes Mellitus, Muscle
The effect of matrix morphology on dynamic-mechanical properties of polypropylene/layered silicate nanocomposites
Authors: Bogoeva-Gaceva Gordana , Raka Lujleta , Sorrentino Andrea
Published: December 2017

In this work, the influence on the morphology and viscoelastic behavior of polypropylene/clay nanocomposites of clay, in combination with different crystallization rates applied in compression molding, is reported. By deconvolution of differential scanning calorimetry (DSC) melting endotherms, it was found that the slowly cooled samples had slightly higher melting temperatures, and the crystal dimensions decreased progressively with the clay content; while, in contrast, the presence of clay particles had no influence on the crystal dimensions in fast-cooled samples. Dynamic mechanical thermal analysis (DMTA) has shown that above the glass transition temperature, nanocomposites obtained by slow cooling exhibited better mechanical response compared to the fast-cooled samples. The value of dynamic modulus E’ of slow-cooled samples increased by ~55 % with addition of only 1 wt% clay, which was attributed to the better reinforcing effect achieved during prolonged time of crystallization.
Nugget Tahu Formula Pury (Tafory) sebagai Alternatif Kudapan Tinggi Protein
Authors: Rifqi Mahmud Aditya , Kusharto Clara M. , Astuti Trina
Published: December 2017

Background: Tofu nugget with pury flour is an alternative form of snack food that high in protein. Pury flour made from silkworm pupa that came from by product of silk industry. Pupa is known to have highly nutritious component specially  protein that has not been optimizely used. The use of pupa flour is applicable for many form of processed food. Objective: Tofu nugget as soure of plant protein is added by pury flour to higher the protein component. Methods: This study used one factor Complete Randomized design with six levels. Level on additon pupa flour are 0%, 60%, 70%, 80%, 90% and 100% followed by organoleptic test to get chosen product. Results: The chosen product according to the test is the addition of 70% pupa flour that indicated highest best reponse by panelist. Proximate test showed that  subtitution of tofu and pury flour is significanly different  for water component, protein, carbohydrate, and seng. Ash elements, fat, fiber, ferum, and calcium significanlly not different. Taffory nugget contains linolenic acid. Total plate count test showed that taffory nugget is safe according to National Standard. Conclusion: It could be conclude that tafory nugget is potential as snack food with high protein.ABSTRAK Latar belakang: Tofu nugget dengan tepung Pury adalah alternatif bentuk makanan ringan yang tinggi protein. Tepung Pury terbuat dari ulat pupa yang berasal dari hasil samping industri sutra. Pupa dikenal memiliki zat gizi khususnya protein yang belum digunakan secara optimal. Penggunaan tepung pupa berlaku untuk banyak bentuk makanan olahan.Tujuan: Tofu nugget sebagai sumber protein nabati, ditambah dengan tepung Pury lebih tinggi komponen protein.Metode: Penelitian ini menggunakan desain eksperimental dengan rancangan acak lengkap dengan enam taraf. Penambahan tepung pupa adalah 0%, 60%, 70%, 80%, 90% dan 100% diikuti dengan uji organoleptik untuk mendapatkan produk yang dipilih.Hasil: Produk yang dipilih sesuai dengan uji adalah penambahan 70% tepung pupa yang ditunjukkan nilai terbaik oleh panelis. Uji proksimat menunjukkan bahwa substitusi dari tahu dan tepung pury berbeda nyata untuk komponen air, protein, karbohidrat, dan seng.  Sedangkan abu, lemak, serat, zat besi dan kalsium tidak berbeda secara signifikan. Nugget Taffory mengandung asam linolenat.Kesimpulan: Dapat disimpulkan bahwa tafory nugget dapat menjadi alternatif kudapan tinggi protein bagi anak.
EFFECT OF HYDROXYETHYL STARCH 200/0.5 ON VON WILLEBRAND FACTOR SERUM LEVEL AND ACTIVATED PARTIAL THROMBOPLASTIN TIME (APTT)
Authors: Atmaja Sarah Puspita , Khotib Junaidi , Rahardjo Eddy , Shinta Dewi Wara , Rahmadi Mahardian , Suprapti Budi
Published: December 2017

Hydroxyethyl starch (HES) is a colloid administered frequently for intravascular volume expansion during perioperative period. Impairment of haemostasis have been reported during HES administration, but the volume of solution administered was usually higher than 20 ml.kg-1. The objective of this study was to evaluate the effect of Hydroxyethyl starch 200/0.5 dose less than 20 ml.kg-1 on von Willebrand factor serum level and activated partial thromboplastin time. A prospective, observational study was conducted to evaluate von Willebrand factor and activated partial thromboplastin time of patients receiving Hydroxyethyl starch 200/0.5. Inclusion criteria were patients undergoing elective surgery who were going to receive Hydroxyethyl starch 200/0.5 intraoperatively. Fourty six patients were divided into patients receiving crystalloid only group (n=23 patients) and hydroxyethyl starch (n=23 patients). Coagulation variables were assesed 30 minute after insicion and 60 minute after infusion of crystalloid or colloid. Measurement of von Willebrand within each group after crystalloid or HES 200 infusion showed significant decrease, from (mean±SE) 97.688±15.219 ng/ml to 31.611±10.058 ng/ml (p< 0.001) in crystalloid group and 92.884±15.208 ng/ml to 27.378±6.399 ng/ml (p<0.001) in HES 200 group. Activated partial thromboplastin time change was statistically significant (mean±SE) 31.27±1.39 to 35.61±1.62 in HES group only (p=0.007), but this change was not clinically significant. In conclusion, there was neither significant difference in von Willebrand serum level nor in activated partial thromboplastin time between the two groups. There was no coagulation influence with clinically significant effect in the use of HES 20 ml/kg BW in patients undergoing elective surgery.
ETHANOL EXTRACT OF MANGOSTEEN PEEL REDUCES HISTOLOGICAL COUNT OF ALVEOLAR MACROPHAGE AND PULMONARY ALVEOLAR SPACE SIZE IN MALE WHITE RATS (Rattus norvegicus) EXPOSED TO CIGARETTE SMOKE
Authors: Santoso Anna Lewi
Published: December 2017

This study aims to analyze histological alveolar macrophage cell number and width of pulmonary alveolar space white male rats given ethanol extract of mangosteen peel and then exposed to cigarette smoke. Smoking can reduce antioxidant and increases free radicals, so it is recommended increasing the antioxidants found in many mangosteen rind. This research was an experimental laboratory using the randomized post test-only control group design. White rats male were chosen randomly into 5 groups (K1, K2, P1, P2, P3). K1 (control-), only the Na-CMC 0.5%, K2 (control+) given Na-CMC 0.5% solution and smoke, P1, P2, P3 given the ethanol extract of mangosteen rind (50, 100, and 200mg/kg wt) and smoke. The data were tested normality and homogenity. Then they were analyzed with one-way Anova, LSD. Data that are not normally tested with Robust tests – (Brown-Forsythe) and Tamhane’s. Correlation test with Pearson and Spearman. Robust test results, the number of alveolar macrophages, value p=0.000 (p<a). Tamhane’s test results K1 significant with P1, P2, P3. P1 significant with P3. Spearman correlation test negative correlation between dose and the number of alveolar macrophage also negative correlation between dose and pulmonary alveolar space. Anova test results, the average variable width of the alveolar spaces, value p=0.000 (p<a). LSD test results are significant differences between the groups, except the group P1 to P2, P3 and P2 to P3. Conclusions of this study is the ethanol extract of mangosteen peel can reduce the number of alveolar macrophage cells and reduce the width of pulmonary alveolar space white male rats were exposed to cigarette smoke.
EFFECTIVENESS OF Betta splendens AS A BIOLOGICAL PREDATORY AGAINST Aedes aegypti LARVAE
Authors: Permata Saniya Hanum , Yotopranoto Subagyo , Kusmartisnawati Kusmartisnawati
Published: December 2017

Dengue Hemorrhagic Fever (DHF) is caused by dengue virus and transmitted by Aedes aegypti mosquito. There are several fish species known to be used as a biological predator of Aedes aegypti larvae, such as Betta splendens. The purpose of this study was to examine Betta splendens as a biological predator of Aedes aegypti larvae. The larvae used were in the fourth stage. The Betta splendens consisted of four replicate groups, 3 and 6 months male age group, and 3 and 6 months female age group. The effectiveness rate of predation was only counted based on the speed of eating of 25 larvae in the aquarium containing 3 liters of water. Statistical test was performed with Annova with the parameters fish body weight and time to prey. The longest mean of preying time was found in 3 months aged male fish group (mean body weight = 1.474 grams), with a mean time of prey = 153.80 seconds. The shortest mean preying time was found ini 6 months aged female fish group (mean body weight = 2.566 grams), with a mean time of prey = 142.41 seconds. In conclusion, female 6 month-aged fish with highest mean body weight is most effective againts Aedes aegypti larvae. The body weight can affect the speed of preying, but body weight is only one of some variables that affect the speed and effectiveness beside gender and age of Betta splendens.
EFFECT OF Cayratia trifolia L (Domin) EXTRACT ON REDUCED EXPRESSION OF MATRIX METALLOPROTEINASE-9 (MMP–9) AND VASCULAR ENDOTHELIAL GROWTH FACTOR –A (VEGF-A) IN WHITE RATS WITH BREAST CANCER
Authors: Dewi Devi Cynthia , Sudiana I Ketut
Published: December 2017

The number of cancer cases in every year has increased. One effort in reducing breast cancer cases is by using anti-angiogenesis which could prevent the growth and metastasis of cancer cells. Xxx the process of angiogenesis and metastasis in cancer cells are associated with the expression of Matrixmetalloproteinase- 9 (MMP-9) and Vascular Endothelial Growth Factor (VEGF). To reduce metastasis in cancer cells, it can be performed by giving plant extracts Cayratiatrifolia L (Domin), containing the active compound Resveratrol, delphinidin/malvidin and Quercetin. The content of the chemical compound of these herbs has immunomodulatory effects, which could potentially prevent angiopoitin, FGF and decrease angiogenesis and neovascularization. So, the metastatic cancer cells can be inhibited. Objective to analyze the effect of the ethanol plant extract Cayratiatrifolia L (Domin) toward the reduction of Matrixmetalloproteinase Expression.-9 (MMP-9) and Vascular Endothelial Growth Factor (VEGF) on white rat of breast cancer models. This study used pure experiment laboratory (True Experimental) with Randomized design study. Post Test was only applied on Control Group Design. This research used experimental female animal Sprague Dawley Rattus which consisted of 30 individuals divided into three groups, from aged 30-40 days. In this research the number of K0 consisted of 5 individual, 6 Kl and 8 KP. KP is a group of experimental animals with breast cancer who were given Cayratiatrifolia extract as much as 300 mg/kg for four weeks. To create animal cancer models, the researcher used DMBA a dose of 10 mg/kg given orally. The measured variables were cells expressing Matrixmetalloproteinase-9 (MMP-9) and Vascular Endothelial Growth Factor (VEGF), differential test applied in this study is Manova. Results based on the statistical analysis of MMP-9 and VEGF-A, between the KI to KP showed a significant difference (p <0.05). Cayratiatrifolia plant extract orally can decrease the number of cells that express MMP-9 and the number of cells that express VEGF-A. The ethanol extract of plants Cayratiatrifolia L (Domain) can reduce the expression Matrixmetalloproteinase-9 (MMP-9) and Vascular endothelial growth factor (VEGF) on white rat of breast cancer models.
EFFECTS OF EDAMAME (Glycine max) EXTRACTON POST-PRANDIAL SERUM TRIGLYCERIDE IN WISTAR RATS
Authors: Kamal Imran H , Dewanti Linda , Wironegoro Rio
Published: December 2017

Edamame (Glycine max) is a preparation of immature soybeans in the pod, which is high in calcium and dietary fiber, two elements described in previous studies that could alter the level of post-prandial serum triglyceride.The purpose of this research is to analyze the effect of edamame (Glycine max) extract on post-prandial serum triglyceride in rats after intragastric administration of palm oil.The materials used in this study are palm oil, edamame extract, and ether. The design of this study is experimental post-test study design, which is a design to measure the serum triglyceride level after meal with and without Glycine max. Triglyceride is measured on each subject two times on total, first one after consumption of meal without Glycine max and the second one after consumption of meal with Glycine max. There is a washout period of 1 week between two times the samples were taken. Both results are then compared in every subject.The level of 2-hour post-prandial serum triglyceride in rats after palm oil without intragastric edamame (Glycine max) extract administration and with intragastric edamame (Glycine max) extract administration showed no significant difference. In conclusion, edamame (Glycine max) extract had no effect on 2-hour post-prandial serum triglyceride after palm oil administration via intragastric tube.
THE POTENCY OF ALPHA LIPOIC ACID AS ANTI INFLAMMATORY ON THE COMPLETE FREUND'S ADJUVANT-INDUCED RHEUMATOID ARTHRITIS IN RAT MODEL
Authors: Megawati Selvi , Rahmadi Mahardian , Susilo Imam , Khotib Junaidi
Published: December 2017

Rheumatoid arthritis (RA) is an autoimmune diseases which is characterized by chronic inflammation of the synovial tissue in joints. This research was designed to investigate the effect of alpha lipoic acid as antioxidant on rats with complete freund’s adjuvant (CFA)-induced RA by intra articular injection of complete freund’s adjuvant (CFA). ALA was administered orally once a day for 7 days at 30, 60 and 120 mg doses a week after CFA injection. The severity of arthritis was evaluated by joint diameter and latency time on thermal stimulation. Joint diameter and latency time on thermal stimulation will measured on day 0, 3, 5, 7, 10, 12 and 14. Measurement of malondialdehyde (MDA) level in plasma was performed using thiobarbituric acid (TBA) method to assess lipid peroxidation. Histology of joint was examined by microscope following hematoxylin-eosin staining. The result showed that treatment with ALA at 30 mg and 60 mg significantly decreased the joint diameter compared to CFA group (p=0.003; p=0.001 respectively) and rat’s latency time on thermal stimulation was also significantly increased compared to CFA group (p=0.015; p=0.026 respectively). Measurement of MDA in CFA group and ALA group had no significant difference. Histological staining indicated that the recovery of the synovial membranes of joint in ALA group had no effect. Results indicated that ALA has the effect to suppress the development of inflammation in RA but not through oxidative stress pathway.
CORRELATION BETWEEN COPING MECHANISM AND SAFETY BEHAVIOR IN CONSTRUCTION WORKERS OF FABRICATION DIVISION IN A STEEL CONSTRUCTION COMPANY
Authors: Widajati Noeroel , Martiana Tri , Mulyono Mulyono
Published: December 2017

Unsafe act become the most cause of accidents. One of human factors causing accidents is work stress. The aim of this study was to analyze correlation between coping mechanism and safety behavior at workers of fabrication division in a steel construction company. The study was carried out with cross-sectional analytical observational methods. The study population was 200 workers of fabrication division in a steel construction company. By using simple random sampling method, the number of samples was 134 respondents. Data collection was performed by observation, interviews, and questionnaires. The independent variables were age, education level, K3 experience, coping mechanism, emotional focused coping, and problem focused coping, while the dependent variable was safety behavior. Intervariables correlation were tested by Chi-square test (α=0.05). Correlation between age and safety behavior was categorized on relation (p=0.016; r=0.301). Correlation between education level and safety behavior was not categorized on relation (p=0.260; r=0.315). Correlation between tenure and safety behavior was categorized on relation (p=0.001; r=0.422). Correlation between coping mechanism and safety behavior was not categorized on relation (p=1.0; r=0.015). The conclusion was age and tenure have unidirectional and strong relation with safety behavior, while the level of education and coping mechanism have no relation with safety behavior. Company should have given understanding to workers about safety behavior through K3 training and safety talk and held safety inspection and or safety patrol to supervise workers.
TWO-PORT LAPAROSCOPIC APPENDECTOMY IS MORE BENEFECIAL THAN OPEN APPENDECTOMY IN EARLY ACUTE APPENDICITIS
Authors: Budipramana Vicky S
Published: December 2017

Open appendectomy has already commonly been performed to treat acute appendicitis, but the relatively more painful and longer incision scar becomes a cosmetical problem. Two port laparoscopic appendectomy can be performed safely, less painful and leaving only two small incision scars. The aim of the study was to evaluate the benefit of two-port laparoscopic appendectomy and that of with open appendectomy in the case of early acute appendicitis. An observational study was conducted from 2012 to 2014; to compare the operating time, post operative pain and wound infection in early acute appendicitis, performed with two-port laparoscopic appendectomy and with open appendectomy. The statistical analysis in this study uses t-test. There were 114 patients (73 men and 41 women) divided into two groups based on the choice made by the patients themselves. The number of samples performed with two-port laparoscopic appendectomy was 64 and that with open appendectomy was 46. Four patients were excluded from this study. In analysis using t-test, we found that the two-port laparoscopic appendectomy was more beneficial than open appendectomy. There was a significant difference between the two groups, the length of operation time was 37.22 minute in two-port laparoscopic appendectomy and 43.83 minute in open appendectomy (p=0.00), VAS pain score was 1.58 in two-port laparoscopic appendectomy and 2.30 in open appendectomy (p=0.00) and no post operative wound infection in both two groups. The conclusion is two-port laparoscopic appendectomy technique is faster and less painful and the same risk of wound infection than open appendectomy in early acute appendicitis.