bluuebunny/crossref_metadata_2025 huggingface dataset:
SAR Signal Formation and Image Reconstruction of a Moving Sea Target
Authors: Lazarov Andon
Published: June 2022

Maritime application of synthetic aperture radar (SAR) technology for sea-target surveillance and imaging is considered in this study. A SAR scenario, including the kinematics of a SAR satellite and a ship moving on the sea, along with the geometry of the target, are analytically described. A linear frequency modulation (LFM) waveform is applied for the target’s illumination. Based on the target’s geometry, SAR and target kinematics and the LFM waveform, a SAR signal model is synthesized. It is proven that the process of signal formation is a transformation of the three-dimensional (3D) image into a two-dimensional (2D) signal, whereas the target’s 2D imaging is an inverse transformation of the 2D signal into the target’s 2D image. SAR signal components, linear Fourier terms and higher-order phase terms are analytically derived and discussed in detail. Moreover, it is proven that SAR image reconstruction is a motion-compensation procedure, i.e., it removes all phases induced by first- and higher-order motion. Based on the SAR signal analysis, an illustrative iterative image-reconstruction algorithm is derived. The quality of the imaging is evaluated by an entropy cost function. Simulation experiments are carried out to verify the correctness of the theoretical statements in respect of SAR signal formation and image reconstruction.
High-Performance Enhancement of SWIR Images
Authors: Dong Sen , Xiong Zhi , Li Rongbing , Chen Yaohong , Wang Hao
Published: June 2022

Short-wave infrared imaging technology has rapidly developed over the past decade. However, image detail and contrast enhancement techniques, which are crucial for infrared imaging systems, are rarely dedicated to SWIR imaging systems. Moreover, the existing IR image enhancement methods generally have difficulty providing real-time performance, which plays a significant role in imaging systems with high data rates. In this paper, we propose a simple and real-time SWIR image enhancement approach based on the difference of Gaussian filter and histogram remapping, and illustrate the implementation of the proposed method on FPGA. The experimental results demonstrated that our method achieves promising detail and contrast enhancement performance with a frame rate of around 50 fps for high-definition images, and that extremely high frame rates can be achieved with pipelined architecture.
Surface Flashover Characteristics of Epoxy Resin Composites in SF6/CF4 Gas Mixture with DC Voltage
Authors: Wang Xin , Wang Zhimin , Chen Jieyuan , Shi Xiaofei , Li Xiaolong
Published: June 2022

SF6/CF4 mixture is considered a potential substitution for SF6 in gas-insulated equipment owing to the considerable insulation and low temperature characteristics of CF4. This paper concerns the substitutability of the mixture on the surface flashover property. Epoxy resin composites for solid insulators are applied since flashover at the insulator interface often occurs and results in severe damage to the safe operation of the equipment. Two kinds of electrode systems are designed to study DC flashover properties with different electric field uniformities. The obtained result shows that the flashover voltage in the 20% SF6/80% CF4 mixture achieved more than 70% of the voltage in SF6 at the same pressure. The mixture shows considerable synergy in the surface flashover process and performed different tendencies with varying gas pressures and contents under different electrode systems due to the influence of surface accumulated charge. Moreover, it is considered that surface charge due to ionization may result in a reversal phenomenon for voltage polarity with increasing pressure. The results indicate the possibility of SF6/CF4 functioning as an efficient substitution of SF6 with a considerable insulation property and improved environmental protection characteristics.
Construction and Calibration of a Unique Hot Box Apparatus
Authors: Alhawari Abdalhadi , Mukhopadhyaya Phalguni
Published: June 2022

A variety of mathematical models are available to estimate the thermal performance of buildings. Nevertheless, mathematical models predict the thermal performance of buildings that might differ from the actual performance. The hot box is a widely-used test apparatus to assess the actual thermal performance of various building envelope components (walls, roofs, windows) in the laboratory. This paper presents the process of designing, constructing, and calibrating a unique small-scale hot box apparatus. Despite its smaller metering area (1.0 m × 1.0 m), this apparatus met the key requirements (below ±0.25 °C fluctuations in chambers’ air temperature, and below 2.0% variation from the point-to-point temperature in reference to the temperature difference across the specimen) as prescribed in the ASTM C1363 and ISO 8990 standards. The walls of this apparatus are uniquely constructed using vacuum insulation panels or VIPs. The efficient and novel use of VIPs and workmanship during the construction of the apparatus are demonstrated through the temperature stability within the chambers. The achieved range of temperature steadiness below ±0.05 °C and point-to-point temperature variation below 1.0% of the temperature difference across the specimen allow for this apparatus to be considered unique among the calibrated hot box categories reported in the literature. In addition, having an affordable, simple-to-operate, and high-accuracy facility offers a great opportunity for researchers and practitioners to investigate new ideas and solutions. The apparatus was calibrated using two extruded polystyrene foam (XPS) specimens with thicknesses of 2″ and 4″. The calibration exercise indicates small differences between results obtained numerically, theoretically, and experimentally (below 3.0%). Ultimately, the apparatus was employed to measure the thermal properties of a specimen representing a lightweight steel framing (LSF) wall system, which is commonly used in cold climates. The results obtained experimentally were then compared to the ones estimated numerically using a 3D finite element modelling tool. The difference between the results obtained by both methods was below 9.0%.
Green Energy Economies Are Continually On-Going
Authors: Hu Jin-Li
Published: June 2022

The Special Issue on “Green Energy Economies” was open for submission on 30 March 2021 and closed on 30 March 2022 [...]
Effect of The Development Level of Facilities for Forest Tourism on Tourists’ Willingness to Visit Urban Forest Parks
Authors: Zhang Liying , Wu Chengliang , Hao Yan
Published: June 2022

The importance of facilities for forest tourism to the development of forest tourism has been mentioned in many studies, but there is a lack of quantitative and specific research. In order to specifically study the effect of facilities for forest tourism on the development of forest tourism, a theoretical path from the cognition of the development level of facilities for forest tourism to the willingness of tourists to visit urban forest parks was constructed based on the perceived value theory. Then, the process and significance of the influence were quantitatively calculated by using a structural equation model (SEM). The results show that: (1) Cognition of the development level of facilities for forest tourism has a significant positive effect on the perceived value of landscape resources, the perceived value of the ecological environment, and tourists’ satisfaction. (2) The perceived value of the ecological environment has a significant positive effect on tourists’ satisfaction. (3) Tourists’ satisfaction has a significant positive effect on tourists’ willingness to visit urban forest parks. (4) The perceived value of landscape resources has no significant effect on tourists’ satisfaction. (5) The variety, layout, and advancement of facilities for forest tourism have a significant positive effect on the cognition of the development level of facilities for forest tourism, among which the layout has the most significant effect, followed by advancement and finally variety. We suggest that, when building facilities for forest tourism, tourists’ feelings and satisfaction should be the primary considerations.
Suitability of Turkey Oak, European Beech, and Hornbeam to Gypsy Moth Feeding
Authors: Milanović Slobodan , Miletić Zoran , Marković Čedomir , Šešlija Jovanović Darka , Trailović Zoran , Jankovský Libor , Lazarević Jelica
Published: June 2022

Pest resistance of trees should be taken into account in future forest strategy planning and predicting risks of defoliation. The gypsy moth (GM) (Lymantria dispar L.) is a serious forest pest with outbreaking population dynamics. To estimate defoliation risk of the most widely distributed tree species in Balkan Peninsula and Europe (Turkey oak Quercus cerris L., European beech Fagus sylvatica L. and hornbeam Carpinus betulus L.), we carried out laboratory feeding trials and investigated their acceptability and suitability for GM development. We determined morphological and chemical attributes of these hosts as well as larval host preference, growth and nutritional indices. Preference, growth, and efficiency of food conversion into biomass were ranked in the order: Turkey oak ˃ European beech ˃ hornbeam. Hornbeam was the most avoided and showed the lowest conversion efficiency although, comparing to optimal oak host, its leaves were less tough, contained more water and exhibited similar values of nitrogen (index of protein content) and C/N ratio (index of investment into carbon based plant defense). We suggest that hornbeam and beech leaf chemical profiles should be further studied to reveal specific compounds that impose high metabolic cost to GM larvae. Moreover, additional research are needed to understand how intermediate hosts in natural populations affect GM outbreaks.
The Role of International Resource Sharing Arrangements in Managing Fire in the Face of Climate Change
Authors: Bloem Sunniva , Cullen Alison C. , Mearns Linda O. , Abatzoglou John T.
Published: June 2022

Changing global fire regimes including extended fire seasons due to climate change may increase the co-occurrence of high-impact fires that overwhelm national fire suppression capacities. These shifts increase the demand for international resource sharing to supplement national fire suppression efforts. In this paper, we explore the development and evaluate the effectiveness of international resource sharing arrangements of three regions: (1) The United States, Canada, and Australia (“Big Three”); (2) Europe; and (3) Southeast Asia by conducting a literature review of gray and peer- reviewed literature in combination with key informant interviews. For the “Big Three” and Europe, international resource sharing is perceived as necessary, effective, and continuously improving. Converging fire management processes and training and developing more effective administrative procedures facilitate these relationships. In Southeast Asia, political tensions and limited firefighting capacities have hampered effective cooperation. Formalized agreements of country-to-country support for fire management are nascent and evolving, and there is evidence that demand for expanding and improving these partnerships is increasing.
A Characterization of Fire-Management Research: A Bibliometric Review of Global Networks and Themes
Authors: Neger Christoph , Rosas-Paz Leonardo Daniel
Published: June 2022

Although humans have interacted with wildfires for millennia, a science-based approach to fire management has evolved in recent decades. This paper reviews the development of fire-management research, focusing on publications that use this term in their title, abstract, or keywords identified on the Scopus platform. This resulted in the identification of 5624 documents published between 1973 and 2021. Publication rates have particularly increased since 2010. The paper details the characteristics of this body of the literature, including the main authors, institutions, and countries. Furthermore, it considers the bibliographic networks, main research foci, and the publications’ study areas. First, these analyses provide researchers interested in fire management an overview of the field and its most prominent sources, authors, and publications. Second, they invite reflection on the current state of fire-management research. In particular, the considerable disparities in spatial foci and countries of authorship suggest that the challenges of today’s problems in fire management are more likely to be overcome with a more balanced global research effort.
Prevalence, Virulence Genes, Phylogenetic Analysis, and Antimicrobial Resistance Profile of Helicobacter Species in Chicken Meat and Their Associated Environment at Retail Shops in Egypt
Authors: Elrais Amina Mohamed , Arab Walid S. , Sallam Khalid Ibrahim , Elmegid Walaa Abd , Elgendy Fatma , Elmonir Walid , Imre Kálmán , Morar Adriana , Herman Viorel , Elaadli Haitham
Published: June 2022

Helicobacter pylori (H. pylori) and Helicobacter pullorum (H. pullorum) are frequently reported pathogens in humans and poultry, respectively. Nevertheless, the source of H. pylori is still unclear. This study aimed to detect Helicobacter spp. in chicken carcasses and to assess the antibiogram and the virulence genes of Helicobacter isolates. Three hundred chicken meat samples (100 each of chicken breast, liver, and gizzard), besides 60 swab samples from chicken processing surfaces, were collected from retail shops in Qalyubia Governorate, Egypt, and examined for the prevalence of H. pylori and H. pullorum. The 16S rRNA of three H. pylori and two H. pullorum isolates were sequenced to determine the genetic relationship between these two Helicobacter spp. Of the 300 chicken samples tested, 16 (5.33%) and 14 (4.67%) were positive for H. pylori and H. pullorum, respectively. Multiplex PCR revealed that the virulence genes vacuolating cytotoxin A (vacA)s1, cytotoxin-associated gene A (cagA), and restriction endonuclease-replacing gene A (hrgA) were detected in 66.7%, 77.8%, and 100% of H. pylori strains tested, respectively. H. pylori showed the highest resistance for clarithromycin, while H. pullorum exhibited the highest resistance towards erythromycin and ciprofloxacin. The study concluded that the chicken meat and giblets are potential sources of the virulent and antimicrobial-resistant strains of H. pylori of human origin.
A Genetic Toxicology Study of the Rapid Detection of Nitrosamine Compounds by the rpsL Gene Mutation Assay
Authors: Peng Xueling , Zhang Junshuai , Liu Qingdai
Published: June 2022

In a rpsL gene mutation experiment, the mutagenicity of the nitrosamine compounds N-diethylnitrosamine (NDEA) and N-dipropylnitrosamine (NDPA) was investigated at the cellular level, as well as with PCR (polymerase chain reaction) and RCA (rolling-circle amplification) amplification systems. The experiments were set up with 10 ppm, 100 ppm, and 1000 ppm concentration gradients of NDEA and NDPA, and ethidium bromide (EB) was used as a positive control group. The results demonstrated that the mutagenic frequency of NDEA and NDPA was significantly higher than the spontaneous mutation frequency of the rpsL gene under the same conditions, but lower than the mutagenic rate of EB in the positive control, and there was a dose-effect relationship, indicating that NDEA and NDPA could induce rpsL gene mutation. The rpsL mutation system has a low spontaneous mutation background and high sensitivity, thus the system is expected to become an effective tool for the rapid detection of carcinogens in the field of food.
Solution of the Ill-Posed Cauchy Problem for Systems of Elliptic Type of the First Order
Authors: Juraev Davron Aslonqulovich , Shokri Ali , Marian Daniela
Published: June 2022

We study, in this paper, the Cauchy problem for matrix factorizations of the Helmholtz equation in the space Rm. Based on the constructed Carleman matrix, we find an explicit form of the approximate solution of this problem and prove the stability of the solutions.
QTL Mapping for Age-Related Eye Pigmentation in the Pink-Eyed Dilution Castaneus Mutant Mouse
Authors: Nakano Takaya , Takenaka Momoko , Sugiyama Makoto , Ishikawa Akira
Published: June 2022

Pink-eyed dilution castaneus (Oca2p-cas) is a mutant gene on mouse chromosome 7 that arose spontaneously in wild Mus musculus castaneus. Homozygotes for Oca2p-cas exhibit pink eyes and a light gray coat throughout life. In an ordinary mutant strain carrying Oca2p-cas, we previously discovered a novel spontaneous mutation that gradually increases melanin pigmentation in the eyes and coat with aging, and we developed a novel mutant strain that was fixed for the novel phenotype. The purpose of this study was to map major quantitative trait loci (QTLs) for the novel pigmentation phenotype and for expression levels of four important melanogenesis genes, microphthalmia-associated transcription factor (Mitf), tyrosinase (Tyr), tyrosinase-related protein-1 (Tyrp1) and dopachrome tautomerase (Dct). We developed 69 DNA markers and created 303 F2 mice from two reciprocal crosses between novel and ordinary mutant strains. The QTL analysis using a selective genotyping strategy revealed a significant QTL for eye pigmentation between 34 and 64 Mb on chromosome 13. This QTL explained approximately 20% of the phenotypic variance. The QTL allele derived from the novel strain increased pigmentation. Although eye pigmentation was positively correlated with Dct expression, no expression QTLs were found, suggesting that the pigmentation QTL on chromosome 13 may not be directly in the pathway of any of the four melanogenesis genes. This study is the first step toward identifying a causal gene for the novel spontaneous phenotype in mice and is expected to discover a new regulatory mechanism for complex melanin biosynthesis during aging.
Ginsenoside Rg5 Sensitizes Paclitaxel—Resistant Human Cervical-Adeno-Carcinoma Cells to Paclitaxel—And Enhances the Anticancer Effect of Paclitaxel
Authors: Ramesh Janani , Thilakan Rejani Chalikkaran , Gopalakrishnan Raja Mohan , Vijayapoopathi Singaravel , Dorschel Arianna , Venugopal Bhuvarahamurthy
Published: June 2022

In cervical cancer chemotherapy, paclitaxel (PTX) chemoresistance has become a major difficulty, and it also affects the survival rate of numerous tumor patients. Thus, for the reversal of chemoresistance, it is imperative to develop combinatory drugs with petite or almost no side effects to sensitize cells to paclitaxel. Ginsenoside Rg5 (GRg5) may act as a chemosensitizer by reversing multidrug resistance. The present study aimed to determine the potential of GRg5 as a chemosensitizer in PTX-resistant human cervical adeno-carcinoma cell lines (HeLa cells). MTT assay was carried out to assess whether GRg5 can potentiate the cytotoxic effect of PTX in PTX- resistant HeLa cells; using flow cytometry-based annexin V-FITC assay, cellular apoptosis was analyzed; the rate of expression of the cell cycle, apoptosis and major cell-survival-signaling-related genes and its proteins were examined using RT-PCR and Western blotting technique. We found increased mRNA expression of Bak, Bax, Bid, and PUMA genes, whereas the mRNA expression of Bcl2, Bcl-XL, c-IAP-1, and MCL-1 were low; GRg5 combination triggered the efficacy of paclitaxel, which led to increased expression of Bax with an enhanced caspase-9/-3 activation, and apoptosis. Moreover, the study supports GRg5 as an inhibitor of two key signaling proteins, Akt and NF-κB, by which GRg5 augments the susceptibility of cervical cancer cells to PTX chemotherapy. GRg5 drastically potentiated the antiproliferative and pro-apoptotic activity of paclitaxel in PTX-resistant human cervical cancer cells in a synergistic mode. Moreover, in the clinical context, combining paclitaxel with GRg5 may prove to be a new approach for enhancing the efficacy of the paclitaxel.
Involvement of MicroRNA-27a-3p in the Licorice-Induced Alteration of Cd28 Expression in Mice
Authors: Feng Gang , Liang Guozheng , Zhang Yaqian , Hu Jicong , Zhou Chuandong , Li Jiawen , Zhang Wenfeng , Shen Han , Wu Fenglin , Tao Changli , Liu Yan , Shao Hongwei
Published: June 2022

Licorice has previously been shown to affect gene expression in cells; however, the underlying mechanisms remain to be clarified. We analyzed the microRNA expression profile of serum from mice treated by gavage with licorice decoction, and obtained 11 differentially expressed microRNAs (DEmiRNAs). We also screened differentially expressed genes (DEgenes) based on RNA-Seq data, and 271 common genes were identified by intersection analysis of the predicted target genes of 11 DEmiRNAs and the DEgenes. The miRNA–gene network showed that most of the hub genes were immune-related. KEGG enrichment analysis of the 271 genes identified three significant pathways, and the 21 genes involved in these three pathways, and the 11 DEmiRNAs, were constructed into a miRNA pathway–target gene network, in which mmu-miR-27a-3p stood out. Compared to ImmPort, there were 13 immune genes within the above group of 21 genes, and three intersected with the mmu-miR-27a-3p predicted target genes, Cd28, Grap2 and Cxcl12, of which the expression of Cd28 changed most significantly. We confirmed the regulation of Cd28 by mmu-miR-27a-3p using a dual-luciferase assay, and further confirmed that overexpression of mmu-miR-27a-3p could significantly downregulate the expression of Cd28 in lymphocytes. These results indicate that mmu-miR-27a-3p could be involved in the licorice-mediated regulation of the expression of Cd28 in mice.
Targeting a Novel G-Quadruplex in the CARD11 Oncogene Promoter with Naptho(2,1-b)furan-1-ethanol,2-nitro- Requires the Nitro Group
Authors: Swafford Kennith , Acharya Baku , Xu Ying-Zhi , Raney Thomas , McCrury Mason , Saha Debasmita , Frett Brendan , Kendrick Samantha
Published: June 2022

The aggressive nature of the activated B cell such as (ABC) subtype of diffuse large B cell (DLBCL) is frequently associated with altered B cell Receptor (BCR) signaling through the activation of key components including the scaffolding protein, CARD11. Most inhibitors, such as ibrutinib, target downstream BCR kinases with often modest and temporary responses for DLBCL patients. Here, we pursue an alternative strategy to target the BCR pathway by leveraging a novel DNA secondary structure to repress transcription. We discovered that a highly guanine (G)-rich element within the CARD11 promoter forms a stable G-quadruplex (G4) using circular dichroism and polymerase stop biophysical techniques. We then identified a small molecule, naptho(2,1-b)furan-1-ethanol,2-nitro- (NSC373981), from a fluorescence-resonance energy transfer-based screen that stabilized CARD11 G4 and inhibited CARD11 transcription in DLBCL cells. In generating and testing analogs of NSC373981, we determined that the nitro group is likely essential for the downregulation of CARD11 and interaction with CARD11 G4, and the removal of the ethanol side chain enhanced this activity. Of note, the expression of BCL2 and MYC, two other key oncogenes in DLBCL pathology with known promoter G4 structures, were often concurrently repressed with NSC373981 and the highly potent R158 analog. Our findings highlight a novel approach to treat aggressive DLBCL by silencing CARD11 gene expression that warrants further investigation.
Epidermal Patterning Factor 2-like (McEPFL2): A Putative Candidate for the Continuous Ridge (cr) Fruit Skin Locus in Bitter Gourd (Momordica charantia L.)
Authors: Yang Jing , Weng Yiqun , Li Huihong , Kong Qiusheng , Wang Weiluan , Yan Chenghuan , Wang Liping
Published: June 2022

Bitter gourd (Momordica charantia L.) is an economically important vegetable and medicinal crop in many Asian countries. Limited work has been conducted in understanding the genetic basis of horticulturally important traits in bitter gourd. Bitter gourd is consumed primarily for its young, immature fruit, and fruit appearance plays an important role in market acceptability. One such trait is the ridges on the fruit skin. In the present study, molecular mapping of a locus underlying fruit ridge continuity was conducted. Genetic analysis in segregating populations, derived from the crosses between two inbred lines Y1 with continuous ridges (CR) and Z-1-4 with discontinuous ridges (DCR), suggested that CR was controlled by a single recessive gene (cr). High-throughput genome sequencing of CR and DCR bulks combined with high-resolution genetic mapping in an F2 population delimited cr into a 108 kb region with 16 predicted genes. Sequence variation analysis and expression profiling supported the epidermal patterning factor 2-like (McEPFL2) gene as the best candidate of the cr locus. A 1 bp deletion in the first exon of McEPFL2 in Y1 which would result in a truncated McEPFL2 protein may be the causal polymorphism for the phenotypic difference between Y1 and Z-1-4. The association of this 1 bp deletion with CR was further supported by gDNA sequencing of McEPFL2 among 31 bitter gourd accessions. This work provides a foundation for understanding the genetic and molecular control of fruit epidermal pattering and development, which also facilitates marker-assisted selection in bitter melon breeding.
Prevention of Blindness in Stickler Syndrome
Authors: Alexander Philip , Snead Martin P.
Published: June 2022

Stickler syndromes are inherited conditions caused by abnormalities of structural proteins in the eye, inner ear and cartilage. The risk of retinal detachment, particularly due to the development of giant retinal tears, is high. Stickler syndrome is the most common cause of childhood retinal detachment. Although retinal detachment surgery in the general population has a high success rate, outcomes from surgical repair in Stickler syndrome patients are notoriously poor, providing a strong argument for prophylactic intervention. Variable case selection, absence of molecular genetic sub-typing and inconsistent treatment strategies have all contributed to the historic uncertainty regarding the safety and efficacy of prophylactic treatment. This paper reviews the major published clinical studies that have evaluated different methods and strategies for prophylaxis. Based on the current body of literature, there is extremely strong evidence from cohort comparison studies demonstrating the efficacy and safety of prophylactic retinopexy to reduce, but not eliminate, the risk of retinal detachment in Stickler syndrome patients. It is vital that this body of evidence is provided to Stickler syndrome patients, to enable them to make their own fully informed choice about whether to receive prophylaxis for themselves and particularly on behalf of their affected children, to reduce the risk of retinal detachment.
Integrated Analyses of DNA Methylation and Gene Expression of Rainbow Trout Muscle under Variable Ploidy and Muscle Atrophy Conditions
Authors: Salem Mohamed , Al-Tobasei Rafet , Ali Ali , Kenney Brett
Published: June 2022

Rainbow trout, Oncorhynchus mykiss, is an important cool, freshwater aquaculture species used as a model for biological research. However, its genome reference has not been annotated for epigenetic markers affecting various biological processes, including muscle growth/atrophy. Increased energetic demands during gonadogenesis/reproduction provoke muscle atrophy in rainbow trout. We described DNA methylation and its associated gene expression in atrophying muscle by comparing gravid, diploid females to sterile, triploid females. Methyl Mini-seq and RNA-Seq were simultaneously used to characterize genome-wide DNA methylation and its association with gene expression in rainbow trout muscle. Genome-wide enrichment in the number of CpGs, accompanied by depleted methylation levels, was noticed around the gene transcription start site (TSS). Hypermethylation of CpG sites within ±1 kb on both sides of TSS (promoter and gene body) was weakly/moderately associated with reduced gene expression. Conversely, hypermethylation of the CpG sites in downstream regions of the gene body +2 to +10 kb was weakly associated with increased gene expression. Unlike mammalian genomes, rainbow trout gene promotors are poor in CpG islands, at <1% compared to 60%. No signs of genome-wide, differentially methylated (DM) CpGs were observed due to the polyploidy effect; only 1206 CpGs (0.03%) were differentially methylated, and these were primarily associated with muscle atrophy. Twenty-eight genes exhibited differential gene expression consistent with methylation levels of 31 DM CpGs. These 31 DM CpGs represent potential epigenetic markers of muscle atrophy in rainbow trout. The DM CpG-harboring genes are involved in apoptosis, epigenetic regulation, autophagy, collagen metabolism, cell membrane functions, and Homeobox proteins. Our study also identified genes explaining higher water content and modulated glycolysis previously shown as characteristic biochemical signs of rainbow trout muscle atrophy associated with sexual maturation. This study characterized DNA methylation in the rainbow trout genome and its correlation with gene expression. This work also identified novel epigenetic markers associated with muscle atrophy in fish/lower vertebrates.
Structural Analysis of microRNAs in Myeloid Cancer Reveals Consensus Motifs
Authors: Dogan Senol , Spahiu Emrulla , Cilic Anis
Published: June 2022

MicroRNAs (miRNAs) are short non-coding RNAs that function in post-transcriptional gene silencing and mRNA regulation. Although the number of nucleotides of miRNAs ranges from 17 to 27, they are mostly made up of 22 nucleotides. The expression of miRNAs changes significantly in cancer, causing protein alterations in cancer cells by preventing some genes from being translated into proteins. In this research, a structural analysis of 587 miRNAs that are differentially expressed in myeloid cancer was carried out. Length distribution studies revealed a mean and median of 22 nucleotides, with an average of 21.69 and a variance of 1.65. We performed nucleotide analysis for each position where Uracil was the most observed nucleotide and Adenine the least observed one with 27.8% and 22.6%, respectively. There was a higher frequency of Adenine at the beginning of the sequences when compared to Uracil, which was more frequent at the end of miRNA sequences. The purine content of each implicated miRNA was also assessed. A novel motif analysis script was written to detect the most frequent 3–7 nucleotide (3–7n) long motifs in the miRNA dataset. We detected CUG (42%) as the most frequent 3n motif, CUGC (15%) as a 4n motif, AGUGC (6%) as a 5n motif, AAGUGC (4%) as a 6n motif, and UUUAGAG (4%) as a 7n motif. Thus, in the second part of our study, we further characterized the motifs by analyzing whether these motifs align at certain consensus sequences in our miRNA dataset, whether certain motifs target the same genes, and whether these motifs are conserved within other species. This thorough structural study of miRNA sequences provides a novel strategy to study the implications of miRNAs in health and disease. A better understanding of miRNA structure is crucial to developing therapeutic settings.
Primitive Cutaneous (P)erivascular (E)pithelioid (C)ell Tumour (PEComa): A New Case Report of a Rare Cutaneous Tumor
Authors: Cazzato Gerardo , Colagrande Anna , Lospalluti Lucia , Pacello Lucia , Lettini Teresa , Arezzo Francesca , Loizzi Vera , Lupo Carmelo , Casatta Nadia , Cormio Gennaro , Maiorano Eugenio , Ingravallo Giuseppe , Resta Leonardo
Published: June 2022

Perivascular epithelioid cell tumours (PEComas) are a growing family of tumours composed of histologically and immunohistochemically distinctive perivascular epithelioid cells. Cutaneous primitive PEComas (cPEComas) are very rare, with 65 cases described in the English literature, and occur as a painless lesion predominantly in female patients, with a wide age range. We present a new case of cPEComa found on the left thigh of a 53-year-old patient with histopathological, immunohistochemical, and molecular information. The lesion was positive for HMB-45 and focal for smooth muscle actin and desmin but negative for melan-A, S-100 protein, CD31, and CD34. Next generation sequencing (NGS) analysis demonstrated the presence of genomic aberration for baculoviral IAP repeats containing BIRC3 splice site 1622-27_1631del37. Although there are little molecular data regarding this entity, our case adds to this knowledge, considering the importance of detecting genomic aberrations in the context of specific therapies such as mTOR inhibitors.
Satellite DNAs in Health and Disease
Authors: Ugarković Đurđica , Sermek Antonio , Ljubić Sven , Feliciello Isidoro
Published: June 2022

Tandemly repeated satellite DNAs are major components of centromeres and pericentromeric heterochromatin which are crucial chromosomal elements responsible for accurate chromosome segregation. Satellite DNAs also contribute to genome evolution and the speciation process and are important for the maintenance of the entire genome inside the nucleus. In addition, there is increasing evidence for active and tightly regulated transcription of satellite DNAs and for the role of their transcripts in diverse processes. In this review, we focus on recent discoveries related to the regulation of satellite DNA expression and the role of their transcripts, either in heterochromatin establishment and centromere function or in gene expression regulation under various biological contexts. We discuss the role of satellite transcripts in the stress response and environmental adaptation as well as consequences of the dysregulation of satellite DNA expression in cancer and their potential use as cancer biomarkers.
Research on Potential Network Markers and Signaling Pathways in Type 2 Diabetes Based on Conditional Cell-Specific Network
Authors: Xie Yuke , Cui Zhizhong , Wang Nan , Li Peiluan
Published: June 2022

Traditional methods concerning type 2 diabetes (T2D) are limited to grouped cells instead of each single cell, and thus the heterogeneity of single cells is erased. Therefore, it is still challenging to study T2D based on a single-cell and network perspective. In this study, we construct a conditional cell-specific network (CCSN) for each single cell for the GSE86469 dataset which is a single-cell transcriptional set from nondiabetic (ND) and T2D human islet samples, and obtain a conditional network degree matrix (CNDM). Since beta cells are the key cells leading to T2D, we search for hub genes in CCSN of beta cells and find that ATP6AP2 is essential for regulation and storage of insulin, and the renin-angiotensin system involving ATP6AP2 is related to most pathological processes leading to diabetic nephropathy. The communication between beta cells and other endocrine cells is performed and three gene pairs with obvious interaction are found. In addition, different expression genes (DEGs) are found based on CNDM and the gene expression matrix (GEM), respectively. Finally, ‘dark’ genes are identified, and enrichment analysis shows that NFATC2 is involved in the VEGF signaling pathway and indirectly affects the production of Prostacyclin (PGI2), which may be a potential biomarker for diabetic nephropathy.
Comment on Mustoe, G.E.; Beard, G. Calcite-Mineralized Fossil Wood from Vancouver Island, British Columbia, Canada. Geosciences 2021, 11, 38
Authors: Haggart James W. , Graham Raymond
Published: June 2022

We are impressed with the detailed work Mustoe and Beard [...]
Writing: The Question as Revolt in Kristeva and Boochani
Authors: Walker Michelle Boulous
Published: June 2022

Writing offers a privileged access to the culture of revolt, a kind of radical questioning that has the potential to unsettle illegitimate forms of authority and sense. Writing bequeaths a future and a society capable of creative thought, and this is all important in societies where questioning and critical thought is increasingly under threat. This work explores the importance of writing in relation to questioning and revolt in two markedly different contexts: in Julia Kristeva’s celebration of the European tradition of revolt and dissent, and in Behrouz Boochani’s literary revolt against the illegitimate incarceration of refugees in Manus Prison. If Kristeva is correct and European culture is, in part, a culture of the question and of revolt, then what does this mean for the non-European world? Boochani’s writing offers a powerful contemporary response to this question, a response that positions the suffering body as a locus of protest and resistance.
Indigenous and Ecofeminist Reclamation and Renewal: The Ghost Dance in Silko’s Gardens in the Dunes
Authors: McNeil Elizabeth
Published: June 2022

Early in the development of ecofeminist literary criticism, white feminists borrowed shallowly and unethically from Indigenous cultures. Using that underinformed discourse to interpret Native American women’s literature resulted in idealizing and silencing Indigenous women’s voices and concerns. Native American feminist literary critics have also asserted that a well-informed, inclusive “tribal-feminism” or Indigenous-feminist critical approach can be appropriate and productive, in that it focuses on unique and shared imbalances created by white patriarchal colonization, thinking, and ways of being that affect Indigenous and non-Indigenous women and cultures and the environment. In her third novel, Gardens in the Dunes, Leslie Marmon Silko interweaves an ecological critique of white imperialist botanical exploitation of landscapes and Indigenous peoples globally with both a celebration of Native American relationships to the land and Indigenous women’s resourceful resistance and an ecofeminist reclamation of European pagan/Great Goddess iconography, sacred landscapes, and white feminist autonomy. Expanding on earlier Indigenous-feminist readings, this ecofeminist analysis looks at a key trope in Gardens, the Ghost Dance, an environmentally and ancestrally focused nineteenth-century sacred resistance and reclamation rite. Silko’s is a late-twentieth-century literary adaptation/enactment in what is the continuing r/evolution of the Ghost Dance, a dynamic figure in Native American literature and culture.
Vibroacoustic Study in the Neonatal Ward
Authors: Sequí-Canet Jose Miguel , Rey-Tormos Romina del , Alba-Fernández Jesús , González-Mazarías Gema
Published: June 2022

Neonatal wards are often subject to excessive noise pollution. Noise pollution encompasses two concepts, noise and vibration; their main difference being that a noise is heard and a vibration is felt in the body. The latter is what can be transmitted within the incubators of neonatal inpatients. This fact needs to be explored in depth. This work shows the results of the diagnosis of vibrations transmitted within the incubators that could affect neonates admitted to the neonatal unit of the Francesc de Borja University Hospital (Gandía, Spain). For this purpose, the vibrations reaching the neonate head resting area were recorded, taking into account different points, scenarios, days, and time slots. It could be observed that due to the incubator’s motor position, the levels obtained in some scenarios measured in this study exceeded the regulation-specified levels. The conclusion is that the greatest influence on vibrations is the incubator’s own motor, with other possible sources of vibrations, such as the room’s air conditioning, having less influence. Further studies are needed to determine whether this vibration is harmful or beneficial to the neonate.
Comparing Prodrugs with Acyclovir for Treating Postherpetic Neuralgia among Herpes Zoster Patients: A Systematic Review and Meta-Analysis
Authors: Yeh Chung-Hsin , Chang Ko-Shih , Huang Sheng-Shiung , Tsay Shiow-Luan , Tsai Jung-Mei , Wang Ya-Jung
Published: June 2022

Postherpetic neuralgia (PHN) is a common, painful, and long-term complication of herpes zoster (HZ). PHN increases the demand for healthcare services and, previous studies showed that patients who received antiviral agents were less likely to develop PHN. The objective of this study was to compare the efficacy of prodrugs and acyclovir in treating PHN among patients with HZ. The search included the PubMed, Medline, Embase, and Cochrane Center of Register of Controlled Trails databases through February 2022. Clinical trials and randomized controlled trials (RCTs) involving antiviral agent intervention for HZ patients diagnosed with PHN were eligible for inclusion. A meta-analysis was conducted to calculate pooled risk ratios (RRs) with 95% confidence intervals (CIs) with a fix-effect model. Five RCTs with 1147 HZ patients met our eligibility criteria. Our meta-analysis found that there was a significantly lower risk of PHN for members of the prodrugs group (famciclovir and valaciclovir) compared with those who received acyclovir (RR = 0.86, 95%, CI: 0.75 to 0.98, p = 0.03). The review of studies indicated that the efficacy of prodrugs was better than acyclovir for reliving PHN.
Detection of Skin Cancer Based on Skin Lesion Images Using Deep Learning
Authors: Gouda Walaa , Sama Najm Us , Al-Waakid Ghada , Humayun Mamoona , Jhanjhi Noor Zaman
Published: June 2022

An increasing number of genetic and metabolic anomalies have been determined to lead to cancer, generally fatal. Cancerous cells may spread to any body part, where they can be life-threatening. Skin cancer is one of the most common types of cancer, and its frequency is increasing worldwide. The main subtypes of skin cancer are squamous and basal cell carcinomas, and melanoma, which is clinically aggressive and responsible for most deaths. Therefore, skin cancer screening is necessary. One of the best methods to accurately and swiftly identify skin cancer is using deep learning (DL). In this research, the deep learning method convolution neural network (CNN) was used to detect the two primary types of tumors, malignant and benign, using the ISIC2018 dataset. This dataset comprises 3533 skin lesions, including benign, malignant, nonmelanocytic, and melanocytic tumors. Using ESRGAN, the photos were first retouched and improved. The photos were augmented, normalized, and resized during the preprocessing step. Skin lesion photos could be classified using a CNN method based on an aggregate of results obtained after many repetitions. Then, multiple transfer learning models, such as Resnet50, InceptionV3, and Inception Resnet, were used for fine-tuning. In addition to experimenting with several models (the designed CNN, Resnet50, InceptionV3, and Inception Resnet), this study’s innovation and contribution are the use of ESRGAN as a preprocessing step. Our designed model showed results comparable to the pretrained model. Simulations using the ISIC 2018 skin lesion dataset showed that the suggested strategy was successful. An 83.2% accuracy rate was achieved by the CNN, in comparison to the Resnet50 (83.7%), InceptionV3 (85.8%), and Inception Resnet (84%) models.
Tokyo 2020: A Sociodemographic and Psychosocial Characterization of the Portuguese Paralympic Team
Authors: Mira Tânia , Monteiro Diogo , Costa Aldo M. , Morouço Pedro , Matos Rui , Antunes Raúl
Published: June 2022

The importance of practicing sports and its impact on the quality of life of people with disabilities is fundamental. Characterizing subjective well-being, resilience, and social influence in the practice of adapted sports, namely in those who participate in elite sport in Portugal, is truly important to support a set of initiatives to promote higher levels of practice. Thus, this study describes the Portuguese delegation at the Tokyo 2020 Paralympic Games through sociodemographic and psychosocial (positive and negative affect, life satisfaction, resilience, and social support) variables. The study involved 31 of the 33 athletes of the Portuguese Paralympic team aged between 15 and 58 years (34.45 ± 11.7 years), with 21 men and 10 women. Individual-level sociodemographic data gave us a clear insight into the reality of adapted sport in Portugal. The high values of life satisfaction, high positive affect and low negative affect, as well as high levels of resilience and social support seem to be important variables for these athletes. The data from the present study highlighted the importance of understanding the characteristics of Paralympic athletes, in order to better understand the reality of Paralympic sport in Portugal.
Upper-Limb Robot-Assisted Therapy Based on Visual Error Augmentation in Virtual Reality for Motor Recovery and Kinematics after Chronic Hemiparetic Stroke: A Feasibility Study
Authors: Cho Ki-Hun , Hong Mi-Ran , Song Won-Kyung
Published: June 2022

The purpose of this study was to investigate the effect of upper-limb robot-assisted therapy based on visual error augmentation in virtual reality (UL-RAT-VEAVR) for motor recovery and kinematics after chronic hemiparetic stroke. This study applied a single-group pre- and post-intervention study design. A total of 27 stroke survivors (20 males and 7 females; mean age 54.51 years, mean onset duration 12.7 months) volunteered to participate in this study. UL-RAT-VEAVR was performed three times a week for four weeks, amounting to a total of twelve sessions, in which an end-effector-based robotic arm was used with a visual display environment in virtual reality. Each subject performed a total of 480 point-to-point movements toward 3 direction targets (medial, ipsilateral, and contralateral side) in the visual display environment system while holding the handle of the end-effector-based robotic arm. The visual error (distance to the targets on the monitor) in virtual reality was increased by 5% every week based on the subject’s maximum point-to-point reaching trajectory. Upper-limb motor recovery was measured in all subjects using the Fugl–Meyer Assessment (FMA) upper-limb subscale, the Box and Block Test (BBT), and the Action Research Arm Test (ARAT), before and after training. In addition, a kinematic assessment was also performed before and after training and consisted of time, speed, distance, and curvilinear ratio for point-to-point movement. There were significant improvements in both upper-limb motor function and kinematics after 4 weeks of UL-RAT-VEAVR (p < 0.05). Our results showed that the UL-RAT-VEAVR may have the potential to be used as one of the upper-limb rehabilitation strategies in chronic stroke survivors. Future studies should investigate the clinical effects of the error-augmentation paradigm using an RCT design.
COVID-19-Related Mortality amongst Physicians in Italy: Trend Pre- and Post-SARS-CoV-2 Vaccination Campaign
Authors: Modenese Alberto , Loney Tom , Gobba Fabriziomaria
Published: June 2022

Globally, there has been a high burden of COVID-19-related mortality amongst physicians and other healthcare workers during the ongoing SARS-CoV-2 pandemic. Fortunately, anti-COVID-19 vaccination campaigns have helped to protect frontline workers and reduce COVID-19-related mortality amongst this occupational group. We analyzed COVID-19-related mortality data for doctors in Italy and compared the crude mortality rate between March–May 2020 (i.e., the beginning of the pandemic in Italy, with the highest rates of COVID-19-related deaths) and the same time period in March–May 2021 (high vaccination coverage amongst Italian physicians). The mortality rate was 12 times higher in March–May 2020 compared to the same time period after the start of the Italian vaccination campaign. Moreover, there was a strong inverse correlation between the number of deaths and the cumulative number of vaccine doses administered in the Italian population. Although non-pharmaceutical interventions, virus evolution and environmental factors probably had an effect, our analysis clearly supports the hypothesis that the vaccination campaign helped to protect Italian physicians and reduce COVID-19-related mortality. The latest available death trends from September to October 2021 for both physicians and the general population are also in favor of the need for the third vaccine dose, currently underway for the majority of the population at risk.