bluuebunny/crossref_metadata_2025 huggingface dataset:
Screening of Bioactive Compounds from Endophytic Marine-Derived Fungi in Saudi Arabia: Antimicrobial and Anticancer Potential
Authors: Al-Rajhi Aisha M. H. , Mashraqi Abdullah , Al Abboud Mohamed A. , Shater Abdel-Rahman M. , Al Jaouni Soad K. , Selim Samy , Abdelghany Tarek M.
Published: August 2022

Nowadays, endophytic fungi represent a rich source of biological active compounds. In the current study, twelve endophytic fungal species were isolated from Avicennia marina leaves. From the isolates, Aspergillus niger, Penicillium rubens and Alternaria alternata recorded the highest isolation frequency (80%), relative density (12.5%) and antimicrobial activity. The antimicrobial and anticancer activities of P. rubens were more effective than those of A. niger and A. alternata; therefore, its identification was confirmed via the ITS rRNA gene. Filtrate extracts of P. rubens, A. alternata and A. niger were analyzed using GC-MS and showed different detected constituents, such as acetic acid ethyl ester, N-(4,6-Dimethyl-2-pyrimidinyl)-4-(4-nitrobenzylideneamino) benzenesulfonamide, 1,2-benzenedicarboxylic acid, hexadecanoic acid and octadecanoic acid. Filtrate extract of P. rubens exhibited the presence of more compounds than A. alternata and A. niger. Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Aspergillus fumigatus were more inhibited by P. rubens extract than A. alternata or A. niger, with inhibition zones of 27.2 mm, 22.21 mm, 26.26 mm, 27.33 mm, 28.25 mm and 8.5 mm, respectively. We observed negligible cytotoxicity of P. rubens extract against normal cells of human lung fibroblasts (WI-38 cell line), unlike A. alternata and A. niger extracts. Proliferation of prostate cancer (PC-3) was inhibited using P. rubens extract, exhibiting mortality levels of 75.91% and 76.2% at 200 µg/mL and 400 µg/mL of the extract. Molecular docking studies against the crystal structures of C. albicans (6TZ6) and the cryo-EM structure of B. subtilis (7CKQ) showed significant interactions with benzenedicarboxylic acid and N-(4,6-dimethyl-2-pyrimidinyl)-4-(4-nitrobenzylideneamino) benzenesulfonamide as a constituent of P. rubens extract. N-(4,6-dimethyl-2-pyrimidinyl)-4-(4-nitrobenzylideneamino) benzenesulfonamide had the highest scores of −6.04905 kcal/mol and −6.590 kcal/mol towards (6tz6) and (7CKQ), respectively.
The Value of Troponin as a Biomarker of Chemotherapy-Induced Cardiotoxicity
Authors: Sorodoc Victorita , Sirbu Oana , Lionte Catalina , Haliga Raluca Ecaterina , Stoica Alexandra , Ceasovschih Alexandr , Petris Ovidiu Rusalim , Constantin Mihai , Costache Irina Iuliana , Petris Antoniu Octavian , Morariu Paula Cristina , Sorodoc Laurentiu
Published: August 2022

In cancer survivors, cardiac dysfunction is the main cause of mortality. Cardiotoxicity represents a decline in cardiac function associated with cancer therapy, and the risk factors include smoking, dyslipidemia, an age of over 60 years, obesity, and a history of coronary artery disease, diabetes, atrial fibrillation, or heart failure. Troponin is a biomarker that is widely used in the detection of acute coronary syndromes. It has a high specificity, although it is not exclusively associated with myocardial ischemia. The aim of this paper is to summarize published studies and to establish the role of troponin assays in the diagnosis of cardiotoxicity associated with various chemotherapeutic agents. Troponin has been shown to be a significant biomarker in the diagnosis of the cardiac dysfunction associated with several types of chemotherapeutic drugs: anthracyclines, anti-human epidermal growth factor receptor 2 treatment, and anti-vascular endothelial growth factor therapy. Based on the data available at this moment, troponin is useful for baseline risk assessment, the diagnosis of cardiotoxicity, and as a guide for the initiation of cardioprotective treatment. There are currently clear regulations regarding the timing of troponin surveillance depending on the patient’s risk of cardiotoxicity and the type of medication administered, but data on the cut-off values of this biomarker are still under investigation.
Breakthrough COVID-19 in Vaccinated Patients with Haematologic Malignancies—The First Single-Centre Experience from the Czech Republic
Authors: Čerňan Martin , Szotkowski Tomáš , Minařík Jiří , Kolář Milan , Sauer Pavel , Látal Vojtěch , Zapletalová Jana , Papajík Tomáš
Published: August 2022

Vaccination is an important tool in the fight against the COVID-19 pandemic in patients with haematologic malignancies. The paper provides an analysis of the course of breakthrough SARS-CoV-2 infection in a group of vaccinated patients with haematological malignancy and a comparison with a historical cohort of 96 non-vaccinated patients with haematologic malignancies and bone marrow failure syndromes (two patients) in the treatment of COVID-19. A severe or critical course of COVID-19 was significantly less frequent in the group of vaccinated patients (10.2% vs. 31.4%, p = 0.003). The need for hospitalisation due to COVID-19 was significantly lower in vaccinated patients (27.1% vs. 72.6%, p < 0.0001) and the duration of hospitalisation was significantly shorter (10 vs. 14 days, p = 0.045). Vaccinated patients were insignificantly less likely to require oxygen therapy during infection. COVID-19 mortality was significantly higher in non-vaccinated patients (15.6% vs. 5.1%, p = 0.047). The paper demonstrated a significant positive effect of vaccination against COVID-19 on a less severe clinical course of infection, lower need for hospitalisation and mortality. However, the results need to be evaluated even in the context of new antivirals and monoclonal antibodies against SARS-CoV-2 or virus mutations with different biological behaviour.
Modularity of the Human Musculoskeletal System: The Correlation between Functional Structures by Computer Tools Analysis
Authors: Della Posta Daniele , Branca Jacopo Junio Valerio , Guarnieri Giulia , Veltro Cristiana , Pacini Alessandra , Paternostro Ferdinando
Published: August 2022

Introduction: For many years, anatomical studies have been conducted with a shattered view of the body. Although the study of the different apparatuses provides a systemic view of the human body, the reconstruction of the complex network of anatomical structures is crucial for the understanding of structural and functional integration. Aim: We used network analysis to investigate the connection between the whole-body osteo-myofascial structures of the human musculoskeletal system. Materials and Methods: The musculoskeletal network was performed using the aNETomy® anatomical network with the implementation of the open-source software Cytoscape for data entry. Results: The initial graph was applied with a network consisting of 2298 body parts (nodes) and 7294 links, representing the musculoskeletal system. Considering the same weighted and unweighted osteo-myofascial network, a different distribution was obtained, suggesting both a topological organization and functional behavior of the network structure. Conclusions: Overall, we provide a deeply detailed anatomical network map of the whole-body musculoskeletal system that can be a useful tool for the comprehensive understanding of every single structure within the complex morphological organization, which could be of particular interest in the study of rehabilitation of movement dysfunctions.
Non-Markov-Type Analysis and Diffusion Map Analysis for Molecular Dynamics Trajectory of Chignolin at a High Temperature
Authors: Fujisaki Hiroshi , Suetani Hiromichi , Maragliano Luca , Mitsutake Ayori
Published: August 2022

We apply the non-Markov-type analysis of state-to-state transitions to nearly microsecond molecular dynamics (MD) simulation data at a folding temperature of a small artificial protein, chignolin, and we found that the time scales obtained are consistent with our previous result using the weighted ensemble simulations, which is a general path-sampling method to extract the kinetic properties of molecules. Previously, we also applied diffusion map (DM) analysis, which is one of a manifold of learning techniques, to the same trajectory of chignolin in order to cluster the conformational states and found that DM and relaxation mode analysis give similar results for the eigenvectors. In this paper, we divide the same trajectory into shorter pieces and further apply DM to such short-length trajectories to investigate how the obtained eigenvectors are useful to characterize the conformational change of chignolin.
Multi-Response Optimisation of Automotive Door Using Grey Relational Analysis with Entropy Weights
Authors: Chen Hao , Lu Chihua , Liu Zhien , Shen Cunrui , Sun Menglei
Published: August 2022

Tail-welded blanks (TWBs) are widely used in automotive bodies to improve the structural performance and reduce weight. The stiffness and modal lightweight design optimisation of TWBs for automotive doors was performed in this study. The finite element model was validated through physical experiments. An L27 (312) Taguchi orthogonal array was used to collect the sample points. The multi-objective optimisation problem was transformed into a single-objective optimisation problem based on the grey relational degree. The optimal combination of structural design parameters was obtained for a tail-welded door using the proposed method, and the weight of the door structure was reduced by 2.83 kg. The proposed optimisation method has fewer iterations and a lower computational cost, enabling the design of lightweight TWBs.
Research on Sliding Mode Control Method of Medium and Low Speed Maglev Train Based on Linear Extended State Observer
Authors: Zhu Yehui , Yang Qing , Li Jie , Wang Lianchun
Published: August 2022

As a new mode of transportation, maglev train has a broad prospect. As one of the key technologies of maglev train, suspension control technology has been a hot research field. Maglev train has the characteristics of complex system modeling, strong nonlinear model, unstable open-loop system, and complex and changeable working conditions. To date, the widely used method is still the design of PID controller based on linearized maglev model. However, with the passage of time, its shortcomings become increasingly clear. To achieve better control effect, an adaptive sliding mode control method based on linear extended state observer (LESO) is proposed in this paper, which not only improves the response speed of the system, but also improves the robustness and anti-interference ability of the system, and greatly reduces the impact of external disturbances on the suspension stability. First, the dynamic model of the system is established, the control characteristics of the system are analyzed, and the design method of current loop is proposed to reduce the order of the system model. Then, the traditional sliding mode control based on reaching law is designed for the reduced order system, and the performance and shortcomings of the controller are analyzed. Aiming at the defects of traditional sliding mode control, a sliding mode controller based on linear extended state observer is designed. The total disturbance and unmodeled part of the system are estimated in real time by LESO and eliminated in sliding mode control. It can be proved that the designed controller can ensure the stability of the closed-loop system, the system state can converge asymptotically in the neighborhood near the expected value, and has a very fast convergence speed. At the same time, the system has strong robustness to noise and external disturbances. Finally, the effectiveness of the proposed controller is verified by simulation and experiment.
PIV Measurement and Proper Orthogonal Decomposition Analysis of Annular Gap Flow of a Hydraulic Machine
Authors: Zhao Yiming , Li Yongye , Song Xiaoteng
Published: August 2022

The fluid stress or flow-induced vibration of annular gap flow always has some influence on the stable working conditions of a hydraulic machine. A time-averaged analysis of flow may not have to explicitly acknowledge these factors. Accordingly, a finite-axial-length annular gap was measured via particle image velocimetry (PIV), with inner boundary motion and a stable outer boundary. As a statistic result regarding the fluid stress, the Reynolds stresses soared in the first region, were sustained in the middle region, but decreased at last. The flow had a higher convective transportation intensity in the radial direction than in other directions. Flow diagnostics were also performed by proper orthogonal decomposition (POD). As a result, the coherent structures were found. Then, the power spectrum density (PSD) functions were also calculated for finding the flow-induced vibration characteristics; the functions had high amplitude in the low-frequency domain and low amplitude in the high-frequency domain, with an order of magnitude between the two amplitudes of 10−1 to 10−2. In addition, the frequency was higher at a smaller gap width in the middle-frequency domain, but the condition was the opposite in the high-frequency domain. In conclusion, the fluid stresses were changeable and uneven along the flow direction, and flow-induced vibration obviously existed. Remarkably, the turbulence characteristics of the annular gap flow were not “laminar approximating,” while the diameter ratio of the gap was 0.6 to 0.8.
A Novel and Simplified Extrinsic Calibration of 2D Laser Rangefinder and Depth Camera
Authors: Zhou Wei , Chen Hailun , Jin Zhenlin , Zuo Qiyang , Xu Yaohui , He Kai
Published: August 2022

It is too difficult to directly obtain the correspondence features between the two-dimensional (2D) laser-range-finder (LRF) scan point and camera depth point cloud, which leads to a cumbersome calibration process and low calibration accuracy. To address the problem, we propose a calibration method to construct point-line constraint relations between 2D LRF and depth camera observational features by using a specific calibration board. Through the observation of two different poses, we construct the hyperstatic equations group based on point-line constraints and solve the coordinate transformation parameters of 2D LRF and depth camera by the least square (LSQ) method. According to the calibration error and threshold, the number of observation and the observation pose are adjusted adaptively. After experimental verification and comparison with existing methods, the method proposed in this paper easily and efficiently solves the problem of the joint calibration of the 2D LRF and depth camera, and well meets the application requirements of multi-sensor fusion for mobile robots.
Active Control of Torsional Vibration during Mode Switching of Hybrid Powertrain Based on Adaptive Model Reference
Authors: Chen Xing , Peng Dan , Wu Wei , Liu Hui , Zheng Xunjia
Published: August 2022

When the energy management and coordinated control of the hybrid electric vehicle power system are not proper, torsional vibration problems will occur in various working states, especially in the mode switching process. These vibrations will affect the comfort, economy and emission of vehicles. In order to suppress the torsional vibration, this paper studied the active vibration control algorithm for the hybrid powertrains under the switching process of pure electric mode to hybrid mode. Primarily, the clutch combination process was divided into five stages and the dynamic models of the transmission system of each stage were established, respectively. Moreover, the principle of model reference adaptive control was analyzed. The applicability of the method to the torsional vibration of the driveline during mode switching was described. Furthermore, the clutch free displacement phase was used as the reference model. A model reference adaptive torsional vibration controller was built based on the controlled model. Finally, the efficacy of this active method for vibration reduction was simulated. The simulation results show that torsional vibration is most likely to occur in the speed coordination stage and the full participation stage. In these two stages, the designed controller can reduce the fluctuation of motor speed by 93.2% and 97.5%, respectively, the engine speed by 79.6% and 77.4%, respectively, the motor acceleration by 96.7% and 82.3%, respectively, and the engine acceleration by 88.9% and 82.3%, respectively. In addition, the controller can reduce the impact degree of the transmission system to within ±1 m/s3.
On Transmission Irregular Cubic Graphs of an Arbitrary Order
Authors: Bezhaev Anatoly Yu. , Dobrynin Andrey A.
Published: August 2022

The transmission of a vertex v of a graph G is the sum of distances from v to all the other vertices of G. A transmission irregular graph (TI graph) has mutually distinct vertex transmissions. In 2018, Alizadeh and Klavžar posed the following question: do there exist infinite families of regular TI graphs? An infinite family of TI cubic graphs of order 118+72k, k≥0, was constructed by Dobrynin in 2019. In this paper, we study the problem of finding TI cubic graphs for an arbitrary number of vertices. It is shown that there exists a TI cubic graph of an arbitrary even order n≥22. Almost all constructed graphs are contained in twelve infinite families.
Constrained Optimal Control for Nonlinear Multi-Input Safety-Critical Systems with Time-Varying Safety Constraints
Authors: Wang Jinguang , Qin Chunbin , Qiao Xiaopeng , Zhang Dehua , Zhang Zhongwei , Shang Ziyang , Zhu Heyang
Published: August 2022

In this paper, we investigate the constrained optimal control problem of nonlinear multi-input safety-critical systems with uncertain disturbances and time-varying safety constraints. By utilizing a barrier function transformation, together with a new disturbance-related term and a smooth safety boundary function, a nominal system-dependent multi-input barrier transformation architecture is developed to deal with the time-varying safety constraints and uncertain disturbances. Based on the obtained transformation system, the coupled Hamilton–Jacobi–Bellman (HJB) function is established to obtain the constrained Nash equilibrium solution. In addition, due to the fact that it is difficult to solve the HJB function directly, the single critic neural network (NN) is constructed to approximate the optimal performance index function of different control inputs, respectively. It is proved theoretically that, under the influence of uncertain disturbances and time-varying safety constraints, the system states and neural network parameters can be uniformly ultimately bounded (UUB) by the proposed neural network approximation method. Finally, the effectiveness of the proposed method is verified by two nonlinear simulation examples.
A Continuous-Time Semi-Markov System Governed by Stepwise Transitions
Authors: Barbu Vlad Stefan , D’Amico Guglielmo , Makrides Andreas
Published: August 2022

In this paper, we introduce a class of stochastic processes in continuous time, called step semi-Markov processes. The main idea comes from bringing an additional insight to a classical semi-Markov process: the transition between two states is accomplished through two or several steps. This is an extension of a previous work on discrete-time step semi-Markov processes. After defining the models and the main characteristics of interest, we derive the recursive evolution equations for two-step semi-Markov processes.
Blockchain and Double Auction-Based Trustful EVs Energy Trading Scheme for Optimum Pricing
Authors: Kakkar Riya , Gupta Rajesh , Agrawal Smita , Bhattacharya Pronaya , Tanwar Sudeep , Raboaca Maria Simona , Alqahtani Fayez , Tolba Amr
Published: August 2022

Electric vehicles (EVs) have gained prominence in smart transportation due to their unparalleled benefits of reduced carbon footprints, improved performance, and intelligent energy trading mechanisms. These potential benefits have increased EV adoption at massive scales, but energy management in EVs is a critical study problem. The problem is further intensified due to the scarcity of charging stations (CSs) in near EV proximity. Moreover, as energy transactions occur over open channels, it presents critical security, privacy, and trust issues among decentralized channels. To address the open limitations of trusted energy management and optimize the pricing control among EV entities (i.e., prosumers and consumers), the paper proposes a scheme that integrates blockchain and a truthful double auction strategy for trustful EV trading. To address the transaction scalability, we integrate an Interplanetary File System (IPFS) with a double auction mechanism handled through the Remix Smart Contract environment. The double auction leverages an optimal payoff condition between peer EVs. To address the communication latency, we present the scheme at the backdrop of Fifth Generation (5G) networks that minimizes the optimal payoff response time. The scheme is simulated against parameters such as convergence, profit for consumers, computation time, and blockchain analysis regarding node commit latency, collusion attacks, and EV energy consumption. The results indicate the scheme’s viability against traditional (non-blockchain) approaches with high reliability, scalability, and improved cost-efficiency.
Simultaneous Design of the Host Structure and the Polarisation Profile of Piezoelectric Sensors Applied to Cylindrical Shell Structures
Authors: Ruiz David , Horta Muñoz Sergio , García-Contreras Reyes
Published: August 2022

Piezoelectric actuators and sensors are applied in many fields in order to produce forces or displacements with the aim of sensing, manipulating or measurement, among other functions. This study presents the numerical methodology to optimize the static response of a thick-shell structure consisting of piezoelectric sensors, based on the maximisation of the electric charge while controlling the amount of piezoelectric and material required. Two characteristic functions are involved, determining the topology of the sensor and the polarisation profile. Constraints over the reaction force are included in the optimisation problem in order to avoid singularities. The topology optimisation method is used to obtain the optimal results, where regularisation techniques (density filtering and projection) are used to avoid hinges. The minimum length scale can be controlled by the use of three different projections. As the main novelty, a displacement-controlled scheme is proposed in order to generate a robust algorithm for future studies including non-linearities.
Forecasting Crude Oil Future Volatilities with a Threshold Zero-Drift GARCH Model
Authors: Liu Tong , Shi Yanlin
Published: August 2022

The recent price crash of the New York Mercantile Exchange (NYMEX) crude oil futures contract, which occurred on 20 April 2020, has caused history-writing movements of relative prices. For instance, the West Texas Intermediate (WTI) experienced a negative price. Explosive heteroskedasticity is also evidenced in associated products, such as the Intercontinental Exchange Brent (BRE) and Shanghai International Energy Exchange (INE) crude oil futures. Those movements indicate potential non-stationarity in the conditional volatility with an asymmetric influence of negative shocks. To incorporate those features, which cannot be accommodated by the existing generalized autoregressive conditional heteroskedasticity (GARCH) models, we propose a threshold zero-drift GARCH (TZD-GARCH) model. Our empirical studies of the daily INE returns from March 2018 to April 2020 demonstrate the usefulness of the TZD-GARCH model in understanding the empirical features and in precisely forecasting the volatility of INE. Robust checks based on BRE and WTI over various periods further lead to highly consistent results. Applications of news impact curves and Value-at-Risk (VaR) analyses indicate the usefulness of the proposed TZD-GARCH model in practice. Implications include more effectively hedging risks of crude oil futures for policymakers and market participants, as well as the potential market inefficiency of INE relative to WTI and BRE.
Assessing Trifecta Achievement after Percutaneous Cryoablation of Small Renal Masses: Results from a Multi-Institutional Collaboration
Authors: Piasentin Andrea , Claps Francesco , Silvestri Tommaso , Rebez Giacomo , Traunero Fabio , Mir Maria Carmen , Rizzo Michele , Celia Antonio , Cicero Calogero , Urbani Martina , Balestreri Luca , Pola Lisa , Laganà Fulvio , Cernic Stefano , Cova Maria Assunta , Bertolotto Michele , Trombetta Carlo , Liguori Giovanni , Pavan Nicola
Published: August 2022

Background and Objectives: To assess efficacy and safety of Percutaneous Cryoablation (PCA) of small renal masses (SRMs) using Trifecta outcomes in a large cohort of patients who were not eligible for surgery. Materials and methods: All PCAs performed in four different centers between September 2009 and September 2019 were retrospectively evaluated. Patients were divided in two different groups depending on masses dimensional criteria: Group-A: diameter ≤ 25 mm and Group-B: diameter > 25 mm. Complications rates were reported and classified according to the Clavien–Dindo system. The estimate glomerular filtration rate (eGFR) was calculated before PCA and during follow-up schedule. Every patient received a Contrast Enhanced Ultrasound (CEUS) evaluation on the first postoperative day. Radiological follow-up was taken at 3, 6, and 12 months for the first year, then yearly. Radiological recurrence was defined as a contrast enhancement persistence and was reported in the study. Finally, Trifecta outcome, which included complications, RFS, and preservation of eGFR class, was calculated for every procedure at a median follow-up of 32 months. Results: The median age of the patients was 74 years. Group-A included 200 procedures while Group-B included 140. Seventy-eight patients were eligible for Trifecta evaluation. Trifecta was achieved in 69.6% of procedures in Group-A, 40.6% in Group-B (p = 0.02). We observed an increased rate of complication in Group-B (13.0% vs. 28.6; p < 0.001). However, 97.5% were <II Clavien–Dindo grade. No differences were found between the two groups regarding eGFR before and after treatment. Further, 24-months RFS rates were respectively 98.0% for Group-A and 92.1% in Group-B, while at 36 months were respectively 94.5% and 87.5% (p = 0.08). Conclusions: PCA seems to be a safe and effective treatment for SRM but in the need of more strict dimensional criteria to achieve a higher possible success rate.
Electrocardiographic Changes Associated with Early Repolarization Pattern in Healthy Young Males
Authors: Frigy Attila , Gábor-Kelemen Hunor , László Szabolcs Attila , Szabó István Adorján , Kocsis Lóránd
Published: August 2022

Background and Objectives: Early repolarization pattern (ERP) has recently been shown to be related with an increased risk of ventricular arrhythmias in susceptible individuals. Materials and Methods: We studied the ERP-associated ECG changes, with potential clinical relevance, in 220 young (age 22.1 ± 1.6 years), healthy, male subjects using 12-lead ECG recordings. A total of 38 subjects (17.3%) fulfilled the diagnostic criteria for ERP, and a total of 90 ECG characteristics were compared between the groups of subjects with and without ERP. Results: None of the ECGs were pathological, and 22 ECG parameters differed significantly (p < 0.05) between the subjects with and without ERP. Among them, the P wave-related parameters (e.g., average P wave duration: 101.5 ± 9.2 ms vs. 106.8 ± 9.9 ms, p = 0.004) and the presence of fragmented QRS complexes (67.6% vs. 92.1%, p = 0.002) revealed a potential propensity for atrial and ventricular arrhythmogenesis. The time-domain parameters of repolarization, those not corrected for QRS duration, showed shorter values (e.g., Tpeak-Tend interval: 70.9 ± 8.1 ms vs. 67.8 ± 8.0 ms, p = 0.036), reflecting the accelerated repolarization. Conclusions: Certain ECG characteristics seem to be more associated with ERP. The clinical significance of this finding at the individual level needs further prospective investigations.
Insomnia Complaints and Perceived Immune Fitness in Young Adults with and without Self-Reported Impaired Wound Healing
Authors: Balikji Jessica , Hoogbergen Maarten M. , Garssen Johan , Roth Thomas , Verster Joris C.
Published: August 2022

Background and Objectives: Adequate sleep and an effective immune system are both essential to maintain a good health status. The current study aimed to determine the nature of insomnia complaints and perceived immune fitness among Dutch young adults with and without self-reported impaired wound healing. Materials and Methods: A total of (n = 2033) Dutch students (83.8% women) completed an online survey. Perceived immune fitness was assessed with a single-item scale and insomnia complaints with the SLEEP-50 insomnia subscale. The sample comprised a control group without self-reported impaired wound healing (n = 1622), a wound infection (WI) group (n = 69), a slow healing wounds (SHW) group (n = 250), and a COMBI group that experienced both WI and SHW (n = 92). Results: Comparisons with the control group revealed that individuals of the SHW and COMBI groups reported significantly poorer perceived immune functioning, increased insomnia complaints and daytime fatigue, and poorer sleep quality. Conclusions: Individuals with self-reported impaired wound healing have a poorer perceived immune functioning, increased insomnia complaints, daytime fatigue, and poorer sleep quality.
Transcranial Electromagnetic Treatment Stops Alzheimer’s Disease Cognitive Decline over a 2½-Year Period: A Pilot Study
Authors: Arendash Gary , Abulaban Haitham , Steen Susan , Andel Ross , Wang Yanhong , Bai Yun , Baranowski Rob , McGarity Jon , Scritsmier Lyle , Lin Xiaoyang , Shen Ning , Aljassabi Ali , Li Yitong , Cao Chuanhai
Published: August 2022

Background: There is currently no therapeutic that can stop or reverse the progressive memory impairment of Alzheimer’s disease (AD). However, we recently published that 2 months of daily, in-home transcranial electromagnetic treatment (TEMT) reversed the cognitive impairment in eight mild/moderate AD subjects. These cognitive enhancements were accompanied by predicted changes in AD markers within both the blood and cerebrospinal fluid (CSF). Methods: In view of these encouraging findings, the initial clinical study was extended twice to encompass a period of 2½ years. The present study reports on the resulting long-term safety, cognitive assessments, and AD marker evaluations from the five subjects who received long-term treatment. Results: TEMT administration was completely safe over the 2½-year period, with no deleterious side effects. In six cognitive/functional tasks (including the ADAS-cog13, Rey AVLT, MMSE, and ADL), no decline in any measure occurred over this 2½-year period. Long-term TEMT induced reductions in the CSF levels of C-reactive protein, p-tau217, Aβ1-40, and Aβ1-42 while modulating CSF oligomeric Aβ levels. In the plasma, long-term TEMT modulated/rebalanced levels of both p-tau217 and total tau. Conclusions: Although only a limited number of AD patients were involved in this study, the results suggest that TEMT can stop the cognitive decline of AD over a period of at least 2½ years and can do so with no safety issues.
GC-TOF/MS-Based Metabolomics for Comparison of Volar and Non-Volar Skin Types
Authors: Bu Ting , Zhang Ming , Lee Sun-Hee , Cheong Yu Eun , Park Yukyung , Kim Kyoung Heon , Kim Dongwon , Kim Sooah
Published: August 2022

Skin has heterogenous identities on different body sites despite similar cellular compositions. There are two types of skin, volar (palmoplantar) and non-volar (dorsal), which are characterized by epidermal thickness, pigmentation, and presence of hair follicles. However, the mechanisms underlying the development of these different skin types remain unclear. To investigate these, we profiled the cellular metabolites of volar and non-volar skin in mice using gas chromatography-time-of-flight/mass spectrometry (GC-TOF/MS), and further assessed the metabolic differences between them. In total, 96 metabolites from both volar and non-volar skin of mice were identified using the BinBase database system. Metabolomics analysis revealed important differences associated with amino acid metabolism (phenylalanine, tyrosine, and tryptophan biosynthesis; aspartate and glutamate metabolism), sugar metabolism (pentose phosphate pathway), and nucleotide metabolism (pyrimidine metabolism) in volar skin. Fifty metabolites were identified as potential biomarkers differentiating the physiological characteristics of these skin types. Of these, nine were highly increased whereas 41 were significantly decreased in volar skin compared with those in non-volar skin. Overall, these results provide valuable information for understanding the metabolic differences between volar and non-volar skin.
Insight into Potential Interactions of Thyroid Hormones, Sex Hormones and Their Stimulating Hormones in the Development of Non-Alcoholic Fatty Liver Disease
Authors: Fröhlich Eleonore , Wahl Richard
Published: August 2022

Non-Alcoholic Fatty Liver Disease (NAFLD) is a common manifestation of metabolic syndrome. In addition to lifestyle, endocrine hormones play a role in the dysregulation of hepatic metabolism. The most common endocrine hormones contributing to metabolic syndrome are alterations in the levels of thyroid hormones (THs, predominantly in subclinical hypothyroidism) and of sex hormones (in menopause). These hormonal changes influence hepatic lipid and glucose metabolism and may increase hepatic fat accumulation. This review compares the effects of sex hormones, THs and the respective stimulating hormones, Thyroid-Stimulating Hormone (TSH) and Follicle-Stimulating Hormone (FSH), on the development of hepatosteatosis. TSH and FSH may be more relevant to the dysregulation of hepatic metabolism than the peripheral hormones because metabolic changes were identified when only levels of the stimulating hormones were abnormal and the peripheral hormones were still in the reference range. Increased TSH and FSH levels appear to have additive effects on the development of NAFLD and to act independently from each other.
Stability Boundary and Enhanced Solution of Dual-Mode Based Micro Gyroscope Mode Matching Technology
Authors: Xing Changda , Wang Xinning , Wang Zishuo , Wang Yuchen , Li Chong
Published: August 2022

During in-run mode matching under a dual-mode gyro scheme, the stability of the closed-loop control system has a boundary. This phenomenon will lead to the failure of the in-run frequency split calibration scheme when the initial mode mismatch is too severe to exceed the stability boundary. This paper gives a detailed analysis of this stability boundary through simulations and experiments. Results show that the length of the stable region will be affected by the resonant frequency and the Q value. High resonant frequency and low Q value will widen the stable region, but also reduce the sensitivity and rapidity of the calibration. In order to remove the limitation of the stability boundary while applying the in-run frequency split calibration under dual-mode architecture, this paper proposes an enhanced solution that combines both the dual-mode scheme and technology of mode switching. The application of mode switching achieves a pre-calibration of frequency split before the normal gyro operation. This solution is implemented in engineering on a hybrid gyro interface circuit prototype with single-mode and dual-mode. Validation experiments confirmed the effectiveness of this solution.
Parasitic Protists: Diversity of Adaptations to a Parasitic Lifestyle
Authors: Kolářová Iva , Florent Isabelle , Valigurová Andrea
Published: August 2022

Parasitic protists cause some of the most well-known human and animal diseases such as malaria, toxoplasmosis, amoebic meningitis, sleeping sickness, leishmaniosis, and diarrheal illness of protozoan origin (e [...]
Whole Genome Sequencing and Molecular Analysis of Carbapenemase-Producing Escherichia coli from Intestinal Carriage in Elderly Inpatients
Authors: Giufrè Maria , Errico Giulia , Monaco Monica , Del Grosso Maria , Sabbatucci Michela , Pantosti Annalisa , Cerquetti Marina , Pagnotta Michela , Marra Manuela , Carollo Maria , Rossini Angelo , Fogato Elena , Cesana Elisabetta , Gentiloni Silverj Flaminia , Zabzuni Dorjan , Tinelli Marco
Published: August 2022

The spread of carbapenemase-producing (CP) Enterobacterales is currently a worldwide concern, especially in the elderly. Twelve CP-E. coli isolated from rectal swabs of colonized inpatients aged ≥65 years from four hospitals in two Italian cities (Milan and Rome) were analyzed by whole genome sequencing (WGS) to obtain multi-locus sequence typing (MLST), identification of carbapenemase-encoding genes, resistome, plasmid content, and virulence genes. MLST analysis showed the presence of 10 unrelated lineages: ST410 (three isolates from three different hospitals in two cities) and ST12, ST38, ST69, ST95, ST131, ST189, ST648, ST1288, and ST1598 (one isolate each). Most isolates (9/12, 75%) contained a serine-β-lactamase gene (5 blaKPC-3, 2 blaKPC-2, and 2 blaOXA-181), while three isolates harbored a metallo-β-lactamase gene (two blaNDM-5 and one blaVIM-1). In most CP-E. coli, the presence of more than one plasmid was observed, with the predominance of IncF. Several virulence genes were detected. All isolates contained genes enhancing the bacterial fitness, such as gad and terC, and all isolates but one, fimH, encoding type 1 fimbriae. In conclusion, CP-E. coli clones colonizing elderly patients showed heterogeneous genetic backgrounds. We recommend strict surveillance to monitor and prevent the spread of successful, high-risk clones in healthcare settings.
Chromatic Acclimation Processes and Their Relationships with Phycobiliprotein Complexes
Authors: Wang Fanyue , Chen Min
Published: August 2022

Chromatic acclimation (CA) is a widespread mechanism for optimizing the composition of phycobiliprotein complexes to maximize the cyanobacterial light capture efficiency. There are seven CA types, CA1-CA7, classified according to various photoregulatory pathways. Here, we use sequence analyses and bioinformatics to predict the presence of CA types according to three GAF (cGMP phosphodiesterase/adenylyl cyclase/FhlA)-containing photoreceptors, CcaS (cyanobacterial chromatic acclimation sensor), RcaE (regulator of chromatic adaptation), and RfpA (regulator for far-red photoacclimation). These photoreceptors were classified into three different phylogenetic groups leading different CA types in a diverse range of cyanobacteria. Combining with genomic information of phycobilisome compositions, the CA capabilities of various cyanobacteria were conjectured. Screening 65 accessible cyanobacterial genomes, we defined 19 cyanobacteria that have the capability to perform far-red light photoacclimation (FaRLiP) under the control of RfpA. Forty out of sixty-five cyanobacteria have the capability to perform green/red light photoacclimation, although they use different photoreceptors (RcaE and/or CcaS) and photoregulatory pathways. The reversible response of photoreceptors in CA regulation pathways trigged by changed light conditions reflects the flexibility of photoregulatory mechanisms in cyanobacteria and the putative independent evolutionary origin of photoacclimation types.
Isolation of Aquatic Plant Growth-Promoting Bacteria for the Floating Plant Duckweed (Lemna minor)
Authors: Makino Ayaka , Nakai Ryosuke , Yoneda Yasuko , Toyama Tadashi , Tanaka Yasuhiro , Meng Xian-Ying , Mori Kazuhiro , Ike Michihiko , Morikawa Masaaki , Kamagata Yoichi , Tamaki Hideyuki
Published: August 2022

Plant growth-promoting bacteria (PGPB) can exert beneficial growth effects on their host plants. Little is known about the phylogeny and growth-promoting mechanisms of PGPB associated with aquatic plants, although those of terrestrial PGPB have been well-studied. Here, we report four novel aquatic PGPB strains, MRB1–4 (NITE P-01645–P-01648), for duckweed Lemna minor from our rhizobacterial collection isolated from Lythrum anceps. The number of L. minor fronds during 14 days co-culture with the strains MRB1–4 increased by 2.1–3.8-fold, compared with an uninoculated control; the plant biomass and chlorophyll content in co-cultures also increased. Moreover, all strains possessed an indole-3-acetic acid production trait in common with a plant growth-promoting trait of terrestrial PGPB. Phylogenetic analysis showed that three strains, MRB-1, -3, and -4, were affiliated with known proteobacterial genera (Bradyrhizobium and Pelomonas); this report is the first to describe a plant-growth promoting activity of Pelomonas members. The gammaproteobacterial strain MRB2 was suggested to be phylogenetically novel at the genus level. Under microscopic observation, the Pelomonas strain MRB3 was epiphytic and adhered to both the root surfaces and fronds of duckweed. The duckweed PGPB obtained here could serve as a new model for understanding unforeseen mechanisms behind aquatic plant-microbe interactions.
Sepiolite and Other Authigenic Mg-Clay Minerals Formation in Different Palustrine Environments (Madrid Basin, Spain)
Authors: Herranz Juan , Pozo Manuel
Published: August 2022

Lithofacies belonging to mud-flat and palustrine deposits (lake margin) in the Miocene of the Madrid Basin (Spain) have been studied. Four lithofacies corresponding to mud flat (1 and 3) and palustrine (2 and 4) deposits have been differentiated. Units 1 and 3 consist mainly of mudstones and carbonates (calcretes and diolocretes). The clay fraction is dominated by trioctahedral smectite (up to 79%) with illite and kaolinite as minor components. The d(060) spacing value shows reflections at 1.52 and 1.50 Å indicating also the presence of dioctahedral phyllosilicates. Unit 2 consists predominantly of lutites (claystones), locally with carbonate and chert nodules. The clay fraction is dominated by sepiolite (up to 96%) with variable contents of smectite and subordinate illite. The d(060) spacing value shows reflections at 1.51 and 1.52 Å indicating trioctahedral clay minerals. Unit 4 consists mostly of carbonates (limestones) with mudstone and lutite inserts. The clay fraction shows different contents of sepiolite, palygorskite and dioctahedral smectite. The analysis of a selection of trace elements (Cr, Co, Th, La, Sc) has allowed us to determine the characteristics of the source area as dioritic, somewhat different from those of the nearby materials from the Batallones sector. Sepiolite shows FWHM values ranging between 0.68 and 1.10 (2θ), indicating “low crystallinity sepiolite”. Differences in the conditions of formation of magnesian smectite and palygorskite have been observed in the mud-flat and palustrine deposits. The formation of sepiolite mainly by neoformation in palustrine deposits with different hydrochemistry is remarkable, leading to differences in fibre size and crystallinity of the fibrous clay mineral. Authigenic transformation processes from previous Al-rich phases would be responsible for the formation of saponite and palygorskite in mud flat and palustrine environments, with different pH conditions.
Characterization, Antiplasmodial and Cytotoxic Activities of Green Synthesized Iron Oxide Nanoparticles Using Nephrolepis exaltata Aqueous Extract
Authors: Nadeem Faisal , Fozia Fozia , Aslam Madeeha , Ahmad Ijaz , Ahmad Shakeel , Ullah Riaz , Almutairi Mikhlid H. , Aleya Lotfi , Abdel-Daim Mohamed M.
Published: August 2022

The use of non-toxic synthesis of iron oxide nanoparticles (FeO NPs) by an aqueous plant extract has proven to be a viable and environmentally friendly method. Therefore, the present investigation is based on the FeO NPs synthesis by means of FeCl3·6H2O as a precursor, and the plant extract of Nephrolepis exaltata (N. exaltata) serves as a capping and reducing agent. Various techniques were used to examine the synthesized FeO NPs, such as UV-Visible Spectroscopy (UV-Vis), Fourier Transform Infrared Spectroscopy (FT-IR), X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), and Energy Dispersive X-ray (EDX). The FT-IR studies were used to identify different photoactive biomolecules at 3285, 2928, 1415, 1170, and 600 cm−1 in the wavenumber range from 4000 to 400 cm−1, indicating the -OH, C-H, C-O, C-C, and M-O groups, respectively. The XRD examination exhibited crystallinity, and the average diameter of the particle was 16 nm. The spherical nature of synthesized FeO NPs was recognized by SEM images, while the elemental composition of nanoparticles was identified by an EDX spectrophotometer. The antiplasmodial activity of synthesized FeO NPs was investigated against Plasmodium parasites. The antiplasmodial property of FeO NPs was evaluated by means of parasite inhibitory concentration, which showed higher efficiency (62 ± 1.3 at 25 μg/mL) against Plasmodium parasite if compared to plant extracts and precursor. The cytotoxicity of FeO NPs was also assessed in human peripheral blood mononuclear cells (PBMCs) under in vitro conditions. The lack of toxic effects through FeO NPs keeps them more effective for use in pharmaceutical and medical applications.
Exploring Core Microbiota Based on Characteristic Flavor Compounds in Different Fermentation Phases of Sufu
Authors: Wu Wei , Wang Zhuochen , Xu Boyang , Cai Jing , Cheng Jianghua , Mu Dongdong , Wu Xuefeng , Li Xingjiang
Published: August 2022

Sufu, a Chinese traditional fermented soybean product, has a characteristic foul smell but a pleasant taste. We determined the core functional microbiota and their metabolic mechanisms during sufu fermentation by examining relationships among bacteria, characteristic flavor compounds, and physicochemical factors. Flavor compounds in sufu were detected through headspace solid-phase microextraction coupled with gas chromatography–mass spectrometry, and the microbial community structure was determined through high-throughput 16S rRNA sequencing. The results showed that the fermentation process of sufu could be divided into early and late stages. The early stage was critical for flavor development. Seven microbiota were screened based on their abundance, microbial relevance, and flavor production capacity. Five microbes were screened in the early stage: Pseudomonas, Tetragenococcus, Lysinibacillus, Pantoea, and Burkholderia–Caballeronia–Paraburkholderia. Three microbes were screened in the late stage: Exiguobacterium, Bacillus, and Pseudomonas. Their metabolic profiles were predicted. The results provided a reference for the selection of enriched bacterial genera in the fermentation process and controlling applicable process conditions to improve the flavor of sufu.
In Vitro and In Vivo Antibacterial Activity, Toxicity and Resistance Analysis of Pleuromutilin Derivative Z33 against Methicillin-Resistant Staphylococcus aureus
Authors: Hu Yuhan , Chen Fang , Zhou Kexin , Zhang Zhe , Li Fei , Zhang Jianfeng , Tang Youzhi , Jin Zhen
Published: August 2022

The novel pleuromutilin derivative, which showed excellent in vitro antibacterial activity against MRSA, 22-(2-(2-(4-((4-(4-nitrophenyl)piperazin-1-yl)methyl)-1H-1,2,3-triazol-1-yl)acetamido)phenyl)thioacety-l-yl-22-deoxypleuromutilin (Z33), was synthesized and characterized in our previous work. In this study, the preliminary pharmacodynamics and safety of Z33 were further evaluated. In in vitro antibacterial activity assays, Z33 was found to be a potent bactericidal antibiotic against MRSA that induced dose-dependent growth inhibition and long-term post-antibiotic effect (PAE). The drug-resistance test demonstrated that Z33 possessed a narrow mutant selection window and lower propensities to select resistance than that of tiamulin. Cytochrome P450 (CYP450) inhibition assay determined that the inhibitory effect of Z33 was similar to that of tiamulin against the activity of CYP3A4, and was lower than that of tiamulin on the activity of CYP2E1. Toxicity determination showed that both Z33 and tiamulin displayed low cytotoxicity of RAW264.7 cells. Furthermore, Z33 was found to be a high-security compound with a 50% lethal dose (LD50) above 5000 mg/kg in the acute oral toxicity test in mice. In an in vivo antibacterial activity test, Z33 displayed better therapeutic effectiveness than tiamulin in the neutropenic mouse thigh infection model. In summary, Z33 was worthy of further development as a highly effective and safe antibiotic agent against MRSA infection.