bluuebunny/crossref_metadata_2025 huggingface dataset:
Primary Chemoradiotherapy Treatment (PCRT) for HER2+ and Triple Negative Breast Cancer Patients: A Feasible Combination
Authors: Ciérvide Raquel , Montero Ángel , García-Rico Eduardo , García-Aranda Mariola , Herrero Mercedes , Skaarup Jessica , Benassi Leticia , Barrera Maria José , Vega Estela , Rojas Beatriz , Bratos Raquel , Luna Ana , Parras Manuela , López María , Delgado Ana , Quevedo Paloma , Castilla Silvia , Feyjoo Margarita , Higueras Ana , Prieto Mario , Suarez-Gauthier Ana , Garcia-Cañamaque Lina , Escolán Nieves , Álvarez Beatriz , Chen Xin , Alonso Rosa , López Mercedes , Hernando Ovidio , Valero Jeannette , Sánchez Emilio , Ciruelos Eva , Rubio Carmen
Published: September 2022

Primary systemic treatment (PST) downsizes the tumor and improves pathological response. The aim of this study is to analyze the feasibility and tolerance of primary concurrent radio–chemotherapy (PCRT) in breast cancer patients. Patients with localized TN/HER2+ tumors were enrolled in this prospective study. Radiation was delivered concomitantly during the first 3 weeks of chemotherapy, and it was based on a 15 fractions scheme, 40.5 Gy/2.7 Gy per fraction to whole breast and nodal levels I-IV. Chemotherapy (CT) was based on Pertuzumab–Trastuzumab–Paclitaxel followed by anthracyclines in HER2+ and CBDCA-Paclitaxel followed by anthracyclines in TN breast cancers patients. A total of 58 patients were enrolled; 25 patients (43%) were TN and 33 patients HER2+ (57%). With a median follow-up of 24.2 months, 56 patients completed PCRT and surgery. A total of 35 patients (87.5%) achieved >90% loss of invasive carcinoma cells in the surgical specimen. The 70.8% and the 53.1% of patients with TN and HER-2+ subtype, respectively, achieved complete pathological response (pCR). This is the first study of concurrent neoadjuvant treatment in breast cancer in which three strategies were applied simultaneously: fractionation of RT (radiotherapy) in 15 sessions, adjustment of CT to tumor phenotype and local planning by PET. The pCR rates are encouraging.
A Selective Histone Deacetylase Inhibitor Induces Autophagy and Cell Death via SCNN1A Downregulation in Glioblastoma Cells
Authors: Chang Hui Hua , Chang Yao-Yuan , Tsai Bing-Chen , Chen Li-Jyun , Chang An-Chi , Chuang Jian-Ying , Gean Po-Wu , Hsueh Yuan-Shuo
Published: September 2022

Glioblastoma multiforme (GBM) is a grade IV, highly malignant brain tumor. Because of the heterogeneity of GBM, a multitarget drug is a rational strategy for GBM treatment. Histone deacetylase inhibitors (HDACis) regulate the expression of numerous genes involved in cell death, apoptosis, and tumorigenesis. We found that the HDAC4/HDAC5 inhibitor LMK235 at 0.5 µM significantly reduced the cell viability and colony formation of patient-derived, temozolomide-resistant GBM P#5 TMZ-R, U-87 MG, and T98G cells. Moreover, LMK235 also significantly increased TUBA acetylation, which is an indicator of HDAC inhibition. Interestingly, LMK235 induced MAP1LC3 robust readout and puncta accumulation but did not enhance PARP1 cleavage or the proportion of annexin V-positive cells, suggesting that LMK235-induced cell death occurred via autophagy activation. Further RNA-seq analysis after LMK235 treatment showed that 597 different expression genes compared to control. After bioinformatic analysis by KEGG and STRING, we focused on 34 genes and validated their mRNA expression by qPCR. Further validation showed that 2 µM LMK235 significantly reduced the mRNA and protein expression of SCNN1A. Cell viability of SCNN1A-silenced cells were reduced, but cells were rescued while treated with an autophagy inhibitor bafilomycin A1. Conclusively, SCNN1A plays a role in LMK235-induced autophagy and cell death in GBM cells.
Protein Profiling of Breast Carcinomas Reveals Expression of Immune-Suppressive Factors and Signatures Relevant for Patient Outcome
Authors: Ruoff Felix , Kersten Nicolas , Anderle Nicole , Jerbi Sandra , Stahl Aaron , Koch André , Staebler Annette , Hartkopf Andreas , Brucker Sara Y. , Hahn Markus , Schenke-Layland Katja , Schmees Christian , Templin Markus F.
Published: September 2022

In cancer, the complex interplay between tumor cells and the tumor microenvironment results in the modulation of signaling processes. By assessing the expression of a multitude of proteins and protein variants in cancer tissue, wide-ranging information on signaling pathway activation and the status of the immunological landscape is obtainable and may provide viable information on the treatment response. Archived breast cancer tissues from a cohort of 84 patients (no adjuvant therapy) were analyzed by high-throughput Western blotting, and the expression of 150 proteins covering central cancer pathways and immune cell markers was examined. By assessing CD8α, CD11c, CD16 and CD68 expression, immune cell infiltration was determined and revealed a strong correlation between event-free patient survival and the infiltration of immune cells. The presence of tumor-infiltrating lymphocytes was linked to the pronounced activation of the Jak/Stat signaling pathway and apoptotic processes. The elevated phosphorylation of PPARγ (pS112) in non-immune-infiltrated tumors suggests a novel immune evasion mechanism in breast cancer characterized by increased PPARγ phosphorylation. Multiplexed immune cell marker assessment and the protein profiling of tumor tissue provide functional signaling data facilitating breast cancer patient stratification.
The Utilization of Two-Phase Catalytic System in Enantioselective Biotransformation of Racemic Atenolol
Authors: Chałupka Joanna , Sikora Adam , Marszałł Michał Piotr
Published: September 2022

There are several methods that allow enantiomerically pure compounds to be obtained. In the study presented herein, the enantioselective biotransformations of (R,S)-atenolol were performed with the use of various catalytic systems containing ionic liquids and toluene as a reaction medium, vinyl acetate as an acetylating agent as well as lipases from Candida rugosa. The conducted studies profs that, the use of the two-phase reaction system enables the reuse of the biocatalyst in another cycle and allows to achieve satisfactory kinetic resolution parameters.
Enhancing Acetophenone Tolerance of Anti-Prelog Short-Chain Dehydrogenase/Reductase EbSDR8 Using a Whole-Cell Catalyst by Directed Evolution
Authors: Zhang Hui , Wang Bei , Yang Shengli , Yu Hongwei , Ye Lidan
Published: September 2022

The short-chain dehydrogenase/reductase (SDR) from Empedobacter brevis ZJUY-1401 (EbSDR8, GenBank: ALZ42979.1) is a promising biocatalyst for the reduction of acetophenone to (R)-1-phenylethanol, but its industrial application is restricted by its insufficient tolerance to acetophenone. In this paper, we developed a chromogenic reaction-based high-throughput screening method and employed directed evolution to enhance the acetophenone tolerance of EbSDR8. The resulting variant, M190V, showed 74.8% improvement over the wild-type in specific activity when catalyzing the reduction of 200 mM acetophenone. Kinetic analysis revealed a 70% enhancement in its catalytic efficiency (kcat/Km). Molecular docking was conducted to reveal the possible mechanism behind the improved acetophenone tolerance, and the result implied that the M190V mutation is conducive to the binding and release of coenzyme. Aside from the improved catalytic performance when dealing with a high concentration of acetophenone, other features of M190V, such as a broad pH range (6.0 to 10.5), low optimal cosubstrate concentration (1% isopropanol), and a temperature optimum close to that of E. coli cells (35 °C), also contribute to its practical application as a whole-cell catalyst. In this study, we first designed a directed evolution means to engineer the enzyme and obtained the positive variant which has a high activity under high concentrations of acetophenone. After that, we optimized the catalytic performance of the variant to adapt to industrial applications.
Proteomics Identifies Substrates and a Novel Component in hSnd2-Dependent ER Protein Targeting
Authors: Tirincsi Andrea , O’Keefe Sarah , Nguyen Duy , Sicking Mark , Dudek Johanna , Förster Friedrich , Jung Martin , Hadzibeganovic Drazena , Helms Volkhard , High Stephen , Zimmermann Richard , Lang Sven
Published: September 2022

Importing proteins into the endoplasmic reticulum (ER) is essential for about 30% of the human proteome. It involves the targeting of precursor proteins to the ER and their insertion into or translocation across the ER membrane. Furthermore, it relies on signals in the precursor polypeptides and components, which read the signals and facilitate their targeting to a protein-conducting channel in the ER membrane, the Sec61 complex. Compared to the SRP- and TRC-dependent pathways, little is known about the SRP-independent/SND pathway. Our aim was to identify additional components and characterize the client spectrum of the human SND pathway. The established strategy of combining the depletion of the central hSnd2 component from HeLa cells with proteomic and differential protein abundance analysis was used. The SRP and TRC targeting pathways were analyzed in comparison. TMEM109 was characterized as hSnd3. Unlike SRP but similar to TRC, the SND clients are predominantly membrane proteins with N-terminal, central, or C-terminal targeting signals.
Magnetic Properties of Nanosized Fe and FeCo Systems on Trenched Mo Templates
Authors: Stanciu Anda Elena , Schinteie Gabriel , Kuncser Andrei Cristian , Locovei Claudiu , Trupina Lucian , Iacob Nicusor , Leca Aurel , Borca Bogdana , Kuncser Victor
Published: September 2022

The manipulation of magnetic anisotropy represents the fundamental prerequisite for the application of magnetic materials. Here we present the vectorial magnetic properties of nanostructured systems and thin films of Fe and FeCo prepared on linearly trenched Mo templates with thermally controlled periodicity. The magnetic properties of the nanosystems are engineered by tuning the shape, size, thickness, and composition parameters of the thin films. Thus, we control coercivity, magnetization, orientation of the easy axis of magnetization, and the long-range magnetic order of the system in the function of the temperature. We distinguish magnetic components that emerge from the complex morpho-structural features of the undulating Fe or FeCo nanostructured films on trenched Mo templates: (i) assembly of magnetic nanowires and (ii) assembly of magnetic islands/clusters. Uniaxial anisotropy at room temperature was proven, characterized, and explained in the case of all systems. Our work contributes to the understanding of magnetic properties necessary for possible further applications of linear systems and undulated thin films.
Approach to the Modification of Carbon-Based Composite Conductive Ink for Silicone Keypads
Authors: Zheng Yujie , Yang Xiutao , Zhao Qianyan , Hao Yaning , Yang Yucheng , Sun Juehan , Tang Junqiang , Zhang Hongguo , Zeng Guanggen
Published: September 2022

Carbon-based composite conductive ink (3CI) has some challenges to its properties. Here, combined with the application of 3CI on silicon keypads, a series of studies on the electrical, mechanical and thermal performance of 3CI has been conducted by adding specific concentrations of silver powder, silica powder and SiO2@Ag core-shell particles. The properties of the modified 3CI were characterized by using the four-point probe tester, scanning electron microscope, Rockwell hardness tester, cross-cut tester and laser thermal conductivity analyzer. The experimental results revealed that by adding silver powder with a particle size of 20 microns equivalent to 12% by weight of the 3CI, ink resistance decreased by 76%, from 8.44 kΩ/□ to 2.03 kΩ/□. Meanwhile, adding silica can increase the ink’s tensile strength and thermal diffusivity while improving the adhesion of the 3CI on the silicone rubber. It was worth noting that when the particle size of the SiO2@Ag core-shell particle was smaller than that of the added silver powder, the resistance of the 3CI was further reduced. Finally, a modified 3CI with the adhesion of 4B, a conductivity of about1 kΩ/□, a hardness of 232 HV0.5, and a thermal diffusivity of 0.217 cm2s−1 was achieved experimentally, which provided an experimental basis for the modified 3CI suitable for silicone keypads.
Feature Fusion Deep Learning Model for Defects Prediction in Crystal Structures
Authors: Alarfaj Abeer Abdulaziz , Hosni Mahmoud Hanan Ahmed
Published: September 2022

Detection of defective crystal structures can help in refute such defective structures to decrease industrial defects. In our research, we are concerned with Silicon nitride crystals. There are four types of crystal structure classes, namely no-defect structures, pristine crystal structures, defective random displacement crystal structures, and defective 25% vacancies crystal structures. This paper proposes a deep learning model to detect the four types of crystal structures with high accuracy and precision. The proposed model consists of both classification and regression models with a new loss function definition. After training both models, the features extracted are fused and utilized as an input to a perceptron classifier to identify the four types of crystal structures. A novel dense neural network (DNN) is proposed with a multitasking tactic. The developed multitask tactic is validated using a dataset of 16,000 crystal structures, with 30% highly defective crystals. Crystal structure images are captured under cobalt blue light. The multitask DNN model achieves an accuracy and precision of 97% and 96% respectively. Also, the average area under the curve (AUC) is 0.96 on average, which outperforms existing detection methods for crystal structures. The experiments depict the computational time comparison of a single training epoch of our model versus state-of-the-art models. the training computational time is performed using crystal structures diffraction image database of twelve image batches. It can be realized that the prediction computational time of our multitasking model is the least time of 21 s.
The Role of the Environment in Shaping the Genomic Variation in an Insular Wild Boar Population
Authors: Fabbri Giulia , Iacolina Laura , Apollonio Marco , Scandura Massimo
Published: September 2022

The Sardinian population of wild boar (WB, Sus scrofa meridionalis) has evolved on this Mediterranean island since its arrival in Neolithic age. Climate and land use vary across the island; high temperatures and dryness represent limiting factors for the development and reproduction of the species. Hence, the environment can have contributed to create the morphological differences we observe today across the island and could sustain the genetic structure that has been previously observed using neutral molecular markers. We therefore searched for genomic signatures of local adaptation in a sample of Sardinian WB genotyped at almost 50 K single nucleotide polymorphisms (SNPs). Genetic structure was observed in the population separating the northwest and southwest from the east of the island, where internal substructure also emerged. We identified 49 SNPs as candidate loci involved in adaptation and 61 genes. Gene ontology enrichment analysis revealed over-representation of terms related to cell localization, motility, and adhesion, but also related to anatomical development and immunity. According to our results, the environment seems to have played a role in shaping the genetic differentiation of the Sardinian wild boar in a limited evolutionary timescale.
Abnormal Proventriculus in Bumble Bee Males
Authors: Ruiz-González Mario X.
Published: September 2022

Females social insects are widely investigated, while males are often neglected. Previous work on the morphology of the bumble bee male alimentary tract has described the presence of swollen proventriculi as a character with taxonomic value. New observations suggest that the abnormal proventriculus might be an abnormal condition. Here, I identify a potential health issue in bumble bee (Hymenoptera: Apidae: Bombus) male alimentary tracts that consists of a swollen and strongly melanized proventriculus, thereby preventing the normal passage of food through the esophageal valve. Males from three bumble bee species exhibited the abnormal proventriculus with an overall prevalence of 31.1% across two years. This finding challenges previous research that suggested the swollen and melanized proventriculus of Bombus males as a taxonomic character.
Multifocal Neuroblastoma and Central Hypoventilation in An Infant with Germline ALK F1174I Mutation
Authors: Djos Anna , Treis Diana , Fransson Susanne , Gordon Murkes Lena , Wessman Sandra , Ásmundsson Jurate , Markström Agneta , Kogner Per , Martinsson Tommy
Published: September 2022

A preterm infant with central hypoventilation was diagnosed with multifocal neuroblastoma. Congenital anomalies of the autonomic nervous system in association with neuroblastoma are commonly associated with germline mutations in PHOX2B. Further, the ALK gene is frequently mutated in both familial and sporadic neuroblastoma. Sanger sequencing of ALK and PHOX2B, SNP microarray of three tumor samples and whole genome sequencing of tumor and blood were performed. Genetic testing revealed a germline ALK F1174I mutation that was present in all tumor samples as well as in normal tissue samples from the patient. Neither of the patient’s parents presented the ALK variant. Array profiling of the three tumor samples showed that two of them had only numerical aberrations, whereas one sample displayed segmental alterations, including a gain at chromosome 2p, resulting in two copies of the ALK-mutated allele. Whole genome sequencing confirmed the presence of the ALK variant and did not detect any aberrations in the coding or promotor region of PHOX2B. This study is to our knowledge the first to report a de novoALK F1174I germline mutation. This may not only predispose to congenital multifocal neuroblastoma but may also contribute to the respiratory dysfunction seen in this patient.
Automatic Screening of Diabetic Retinopathy Using Fundus Images and Machine Learning Algorithms
Authors: Mujeeb Rahman K. K. , Nasor Mohamed , Imran Ahmed
Published: September 2022

Diabetic Retinopathy is a vision impairment caused by blood vessel degeneration in the retina. It is becoming more widespread as it is linked to diabetes. Diabetic retinopathy can lead to blindness. Early detection of diabetic retinopathy by an ophthalmologist can help avoid vision loss and other complications. Diabetic retinopathy is currently diagnosed by visually recognizing irregularities on fundus pictures. This procedure, however, necessitates the use of ophthalmic imaging technologies to acquire fundus images as well as a detailed visual analysis of the stored photos, resulting in a costly and time-consuming diagnosis. The fundamental goal of this project is to create an easy-to-use machine learning model tool that can accurately predict diabetic retinopathy using pre-recorded digital fundus images. To create the suggested classifier model, we gathered annotated fundus images from publicly accessible data repositories and used two machine learning methods, support vector machine (SVM) and deep neural network (DNN). On test data, the proposed SVM model had a mean area under the receiver operating characteristic curve (AUC) of 97.11%, whereas the DNN model had a mean AUC of 99.15%.
An Efficient Retrieval System Framework for Fabrics Based on Fine-Grained Similarity
Authors: Xiang Jun , Pan Ruru , Gao Weidong
Published: September 2022

In the context of “double carbon”, as a traditional high energy consumption industry, the textile industry is facing the severe challenges of energy saving and emission reduction. To improve production efficiency in the textile industry, we propose the use of content-based image retrieval technology to shorten the fabric production cycle. However, fabric retrieval has high requirements for results, which makes it difficult for common retrieval methods to be directly applied to fabric retrieval. This paper presents a novel method for fabric image retrieval. Firstly, we define a fine-grained similarity to measure the similarity between two fabric images. Then, a convolutional neural network with a compact structure and cross-domain connections is designed to narrow the gap between fabric images and similarities. To overcome the problems of probabilistic missing and difficult training in classical hashing, we introduce a variational network module and structural module into the hashing model, which is called DVSH. We employ list-wise learning to perform similarity embedding. The experimental results demonstrate the superiority and efficiency of the proposed hashing model, DVSH.
Forensic Analysis of TikTok Alternatives on Android and iOS Devices: Byte, Dubsmash, and Triller
Authors: Keim Yansi , Hutchinson Shinelle , Shrivastava Apoorva , Karabiyik Umit
Published: September 2022

TikTok has consistently been one of the most used mobile apps worldwide on any mobile operating system. However, despite people’s enjoyment of using the application, there have been growing concerns about the application’s origins and alleged privacy violations. These allegations have become such a big problem that the former President of the United States, Donald Trump, expressed a desire to ban the TikTok application from being offered on US application stores like Google’s Play Store and Apple’s App Store. This remark sent TikTok users into a frenzy to find alternatives before the ban took effect. To this end, several alternative applications for TikTok have surfaced and are already garnering millions of users. In this paper, we identified three popular alternatives to the TikTok application (Byte, Dubmash, and Triller) and forensically analyzed each on smartphones of Android version 8 and iOS version 13. We focused on identifying forensically relevant artifacts that may be helpful to investigators in the event of a criminal investigation, should these or similar apps fall under scrutiny. We used Magnet AXIOM Process and Cellebrite UFED 4PC for acquisition, and Magnet AXIOM Examine and DB Browser for SQLite for analysis and reading. The investigation resulted in successful extraction of expected yet unique data points, plain text sensitive data, directories and format. These results lead to a discussion about identifying and comparing these app’s privacy concerns to that of TikTok, as formulated from the literature.
Assessment of the Impact of Renewable Energy Expansion on the Technological Competitiveness of the Cogeneration Model
Authors: Im Yonghoon
Published: September 2022

The rapid transition from an efficiency-oriented to a renewable energy-based green environment raises questions about the sustainability of cogeneration models in the coming era of climate change. For securing the technological competitiveness of a cogeneration model in terms of sustainability, it is essential to come up with alternatives that can flexibly respond to changes in the market conditions. From the surveyed field operation data of the cogeneration model applied to an apartment complex, it was found that the actual operation performance may differ significantly from the theoretical expectation. Through diagnostic simulation analysis, the main cause of the disappointing performance in the case of the current cogeneration model after installation has been assessed, and the importance of a consistent operation strategy was demonstrated by the event-based correlation analysis based on field operation data. The impact of the rapid expansion and dissemination of the renewable energy market on the relative primary energy savings benefit evaluation of the cogeneration model was analyzed for various operating conditions.
Re-Defining System LCOE: Costs and Values of Power Sources
Authors: Matsuo Yuhji
Published: September 2022

The mass introduction of variable renewable energies, including wind and solar photovoltaic, leads to additional costs caused by the intermittency. Many recent studies have addressed these “integration costs,” and proposed novel metrics that replace the traditional metric known as the levelized cost of electricity (LCOE). However, the policy relevance of those metrics remains unclear. In this study, the author investigates and re-defines the concept of system LCOE, referring to prior studies, and proposes concrete methods to estimate them. Average system LCOE allocates the integration cost to each power source, dividing that by the adjusted power output. Marginal system LCOE revises the concept of system LCOE and value-adjusted LCOE proposed by prior studies, to be clearer and more policy-relevant. These metrics are also applied to Japan’s power sector in 2050, suggesting the necessity of aiming for a “well-balanced energy mix” in future power systems with decarbonised power sources.
Water Recuperation from Hydrogen Fuel Cell during Aerial Mission
Authors: Zakhvatkin Lev , Schechter Alex , Avrahami Idit
Published: September 2022

A water recuperation system (WRS) from an open-cathode proton exchange membrane fuel cell (PEMFC) is designed to increase the energy density of hydrogen production by hydrolysis of metal hydrides. WRS may significantly reduce the water weight in the carried fuel. The design is based on circulating the humid air through the PEMFC stack in a closed dome. To ensure oxygen supply to the PEMFC, the WRS has a ventilation inlet and an exhaust outlet. The required conditions for ventilation flow are developed theoretically and examined experimentally in a WRS prototype with a commercial PEMFC at 20–100 W. The experimental system includeds a closed dome, an edge cooling system for the PEMFC, a controllable ventilation air inlet, and an exhaust port. The humid exhaust air was cooled down to the ambient temperature to improve vapor condensation. Results show high efficiency (80% recuperated water from prediction), with a potential to achieve gravimetric hydrogen storage capacity (GHSC) of >6 wt% at an ambient temperature of 27 °C. The described principle may be applied for small fixed-wing drones where the cold ambient air may be utilized both for providing oxygen supply and for thermal management of the PEMFC and the humid exhaust, thus allowing higher GHSC.
The Study for Technical Analysis on the Development Potential of Multi-Zone Oil, Gas in Crossfield, Canada
Authors: Moon Bryan Sangho , Lee Wangdo , Lee Youngsoo
Published: September 2022

Crossfield is located in the province of Alberta in Canada and is one of the most well-known reservoirs of hydrocarbons. Since the 1950s, there has been continuous exploitation of more than 10 formations such as Cardium, Viking, Ellerslie, Elkton, Shunda, and Wabamun. Because of its location near the Foothills, the southwestern part of the Western Canada Sedimentary Basin, the Crossfield area has a complex geology and relatively deeper reservoirs, therefore requiring an in-depth examination of each formation. In this study, geological, technical, and economic analyses were performed on each formation within the 10 sections of the study lands in the Crossfield area. As the result of the study, there was potential for drilling 48 horizontal wells in the Cardium A zone and the Viking formation. In addition, it turned out to be economically feasible even at the WTI price of 60 dollars per barrel considering Alberta’s Royalty Framework, which is determined by the production rate and the price of oil, and the Horizontal Oil New Well Royalty Rate feature. A further study is required to investigate the exploitation potential of the Cardium B zone, the Ellerslie, and the Elkton formations in this area.
Localization of TWISTED NEEDLES Locus on Linkage Map of Japanese Cedar (Cryptomeria japonica D. Don)
Authors: Moriguchi Yoshinari , Saito Ryunosuke , Ueno Saneyoshi , Hasegawa Yoichi , Kakui Hiroyuki , Matsumoto Asako
Published: September 2022

Sugi (Cryptomeria japonica D. Don) is an important forestry species in Japan. C. japonica ‘Spiralis’ is a mutant with twisted needles. The mutant is called Yore-sugi in Japan and is known as ‘Rasen’ in other countries. The twisted trait is regulated by a dominant gene called TWISTED NEEDLES, and it can be identified by observing the needles within 1 to 2 months after germination. The TWISTED NEEDLES gene may be useful for improving the efficiency of genome editing technology in C. japonica. In this study, we attempted to identify the linkage group of the TWISTED NEEDLES locus and investigate markers that sandwich this locus using the mapping family. First, we identified the linkage group containing the TWISTED NEEDLES locus based on the distortion from the expected segregation ratio using 32 mutant individuals of the mapping family. The segregation distortion showed that the TWISTED NEEDLES locus was located on the 11th linkage group (LG11). Next, a linkage map of LG11 was constructed based on genotype data from the single-nucleotide polymorphism (SNP) markers and double digested restriction site-associated DNA sequencing (ddRAD-seq) using 123 individuals of the MMY-1 family. On this map, six markers were located at the same position as the TWISTED NEEDLES locus. To investigate markers sandwiching the TWISTED NEEDLES locus, a partial linkage map around the TWISTED NEEDLES locus was constructed using 643 individuals of the MMY-1 family. The TWISTED NEEDLES locus was located in the 0.6 cM region between gSNP01822 and the other five markers (Contig_4705-179, Contig_4518-93, Contig_4398-118, gSNP04056, and Contig_4970-113).
Low Power Blockchained E-Vote Platform for University Environment
Authors: Chaabane Faten , Ktari Jalel , Frikha Tarek , Hamam Habib
Published: September 2022

With the onset of the COVID-19 pandemic and the succession of its waves, the transmission of this disease and the number of deaths caused by it have been increasing. Despite the various vaccines, the COVID-19 virus is still contagious and dangerous for affected people. One of the remedies to this is precaution, and particularly social distancing. In the same vein, this paper proposes a remote voting system, which has to be secure, anonymous, irreversible, accessible, and simple to use. It therefore allows voters to have the possibility to vote for their candidate without having to perform the operation on site. This system will be used for university elections and particularly for student elections. We propose a platform based on a decentralized system. This system will use two blockchains communicating with each other: the public Ethereum blockchain and the private Quorum blockchain. The private blockchain will be institution-specific. All these blockchains send the necessary data to the public blockchain which manages different data related to the universities and the ministry. This system enables using encrypted data with the SHA-256 algorithm to have both security and information security. Motivated by the high energy consumption of blockchain and by the performance improvements in low-power, a test is performed on a low-power embedded platform Raspberry PI4 showing the possibility to use the Blockchain with limited resources.
Effectiveness of Different Washing Strategies on Pesticide Residue Removal: The First Comparative Study on Leafy Vegetables
Authors: Yang So-Jin , Mun Sujin , Kim Hye Jin , Han Sue Ji , Kim Do Woo , Cho Bae-Sik , Kim Ae Gyeong , Park Duck Woong
Published: September 2022

Leafy vegetables are used in various cuisines worldwide; however, as they cannot be peeled and their leaf surface area is large, the risk of retaining pesticide residues on these vegetables is relatively higher than on others. To our knowledge, this is the first comparative study to reveal the effect of removing pesticide residues from five artificially contaminated leafy vegetables (lettuce, perilla leaves, spinach, crown daisy, and ssamchoo (Brassica lee ssp. namai)) using different removal methods. The percent reduction range for each method was 43.7–77.0%, and the reduction range for the five leafy vegetables was 40.6–67.4%. Lettuce had the highest reduction (67.4 ± 7.3%), whereas ssamchoo had the lowest reduction (40.6 ± 12.9%). Spinach and crown daisy showed no significant difference in their reductions. Based on reduction by method, running water (77.0 ± 18.0%) and boiling (59.5 ± 31.2%) led to the highest reduction, whereas detergent (43.7 ± 14.5%) led to the lowest reduction. The reductions of chlorfenapyr, diniconazole, indoxacarb, fludioxonil, pyraclostrobin, and lufenuron in the leafy vegetables were lower with blanching and boiling than with other methods (p < 0.05). These results highlight the importance of thoroughly washing leafy vegetables to lower the intake of pesticide residues before cooking.
Nonlinear Soliton-like Oscillations and Waves during Geomaterial Destruction Based on Electromagnetic Radiation Signals
Authors: Borisov Victor Dmitrievich
Published: September 2022

The work is devoted to the results of processing electromagnetic radiation signals obtained during laboratory loading of marble and diabase samples using a technique for determining the parameters of microcracks, developed and published by the author earlier. As a result of such processing, certain patterns were found in the nature of the evolution of the oscillatory process ensemble of microcracks. For example, solitary non-linear waves almost always preceded a sequence of High Frequency traces. Equations for straight lines approximating High Frequency traces in logarithmic coordinates, close to the equation of the Gutenberg–Richter law. Due to the similarity of seismic processes at different scale levels, the results of modeling at the microscale level can be used to describe seismic processes at the macroscale level, for example, to study the processes occurring immediately before destruction and at the time of destruction in order to search for repeatability and regularities. The regularities obtained can be used in the development of a predictive criterion that makes it possible to predict the time of one or another geophysical (seismic) event.
Chronic Calcifying Pancreatitis Associated with Secondary Diabetes Mellitus and Hepatosplenic Abscesses in a Young Male Patient: A Case Report
Authors: Marginean Cristina Maria , Popescu Mihaela , Vasile Corina Maria , Stanciu Mihaela , Popescu Iulian Alin , Biciusca Viorel , Ciobanu Daniela , Dobrescu Amelia , Sandulescu Larisa Daniela , Bondari Simona , Popescu Marian Sorin , Mitrut Paul
Published: September 2022

Background: Chronic pancreatitis (CP) has been described as a multifactorial, ongoing inflammatory condition of the pancreas of varying intensity that produces persistent pain, leading to exocrine and endocrine insufficiency and a decreased lifespan. Currently, there are three primary forms of chronic pancreatitis: chronic autoimmune pancreatitis (steroid-sensitive pancreatitis), chronic obstructive pancreatitis, and chronic calcific pancreatitis, the latter being closely related to excessive alcohol consumption for one or even two decades before the onset of symptoms. Case report: We present the case of a 29 year old man who required medical attention for a significant unintentional weight loss and a history of upper abdominal pain. Blood tests revealed substantial abnormalities, and the patient was admitted for further investigation. CT and MRI confirmed the presence of a pancreatic pseudocyst and extensive pancreatic parenchymal calcifications and revealed multiple hepatosplenic microabscesses of fungal etiology. Conclusions: Chronic calcifying pancreatitis is a complex clinical entity that can lead to secondary diabetes due to progressive destruction of the pancreatic parenchyma. Protein malnutrition, caused by malabsorption syndrome, immune cell dysfunction, and a high glucose environment caused by diabetes mellitus, may create a state of immunodeficiency, predisposing the patient to opportunistic infections.
Variation in Ovine DGAT1 and Its Association with Carcass Muscle Traits in Southdown Sheep
Authors: Dai Rong , Zhou Huitong , Fang Qian , Zhou Ping , Yang Yang , Jiang Shuang , Hickford Jonathan G. H.
Published: September 2022

Diacylglycerol O-acyltransferase 1 (DGAT1) is a microsomal enzyme that plays a key role in the synthesis of triglycerides. Its gene (DGAT1) is regarded as a candidate gene for variation in milk and meat traits in cattle. The objective of this study was to use a PCR single-strand conformation polymorphism approach to explore sequence variation in two regions of ovine DGAT1 and to assess its effect on meat traits in New Zealand Southdown sheep. Three variant nucleotide sequences were identified in each region, with two single nucleotide polymorphisms (SNPs) and one nucleotide deletion being detected in intron 1 and two SNPs being found in exon 17. The effect of the exon 17 variation was not investigated due to one variant being predominant and the other two variants occurring at low frequencies. In intron 1, one variant (B1) was found to be associated with increase loin meat yield, suggesting that this may have value as a gene marker for improving meat traits.
Blood Transcriptome Analysis of Beef Cow with Different Parity Revealed Candidate Genes and Gene Networks Regulating the Postpartum Diseases
Authors: Yang Yanda , Chang Chencheng , Baiyin Batu , Liu Zaixia , Guo Lili , Zhou Le , Liu Bin , Shi Caixia , Zhang Wenguang
Published: September 2022

Maternal parity is an important physiological factor influencing beef cow reproductive performance. However, there are few studies on the influence of different calving periods on early growth and postpartum diseases. Here, we conducted blood transcriptomic analysis on cows of different parities for gene discovery. We used Short Time Series Expression Miner (STEM) analysis to determine gene expression levels in cows of various parities and divided multiple parities into three main periods (nulliparous, primiparous, and multiparous) for subsequent analysis. Furthermore, the top 15,000 genes with the lowest median absolute deviation (MAD) were used to build a co-expression network using weighted correlation network analysis (WGCNA), and six independent modules were identified. Combing with Exon Wide Selection Signature (EWSS) and protein-protein interaction (PPI) analysis revealed that TPCN2, KIF22, MICAL3, RUNX2, PDE4A, TESK2, GPM6A, POLR1A, and KLHL6 involved in early growth and postpartum diseases. The GO and KEGG enrichment showed that the Parathyroid hormone synthesis, secretion, and action pathway and stem cell differentiation function-related pathways were enriched. Collectively, our study revealed candidate genes and gene networks regulating the early growth and postpartum diseases and provided new insights into the potential mechanism of reproduction advantages of different parity selection.
Comparative Analysis of Chloroplast Genomes within Saxifraga (Saxifragaceae) Takes Insights into Their Genomic Evolution and Adaption to the High-Elevation Environment
Authors: Chen Zhuyifu , Yu Xiaolei , Yang Yujiao , Wei Pei , Zhang Wencai , Li Xinzhong , Liu Chenlai , Zhao Shuqi , Li Xiaoyan , Liu Xing
Published: September 2022

Saxifraga species are widely distributed in alpine and arctic regions in the Northern hemisphere. Highly morphological diversity within this genus brings great difficulties for species identification, and their typical highland living properties make it interesting how they adapt to the extreme environment. Here, we newly generated the chloroplast (cp) genomes of two Saxifraga species and compared them with another five Saxifraga cp genomes to understand the characteristics of cp genomes and their potential roles in highland adaptation. The genome size, structure, gene content, GC content, and codon usage pattern were found to be highly similar. Cp genomes ranged from 146,549 bp to 151,066 bp in length, most of which comprised 130 predicted genes. Yet, due to the expansion of IR regions, the second copy of rps19 in Saxifraga stolonifera was uniquely kept. Through sequence divergence analysis, we identified seven hypervariable regions and detected some signatures of regularity associated with genetic distance. We also identified 52 to 89 SSRs and some long repeats among seven Saxifraga species. Both ML and BI phylogenetic analyses confirmed that seven Saxifraga species formed a monophyletic clade in the Saxifragaceae family, and their intragenus relationship was also well supported. Additionally, the ndhI and ycf1 genes were considered under positive selection in species inhabiting relatively high altitudes. Given the conditions of intense light and low CO2 concentration in the highland, the products of these two genes might participate in the adaptation to the extreme environment.
Gene Polymorphism and Total Genetic Score in Martial Arts Athletes with Different Athletic Qualifications
Authors: Vostrikova Anna , Pechenkina Victoria , Danilova Maria , Boronnikova Svetlana , Kalendar Ruslan
Published: September 2022

The personalized approach in sports genetics implies considering the allelic variants of genes in polymorphic loci when adjusting the training process of athletes. The personalized approach is used both in sports genetics and in medicine to identify the influence of genotype on the manifestations of human physical qualities that allow to achieve high sports results or to assess the impact of genotype on the development and course of diseases. The impact of genes of the renin-angiotensin and kinin-bradykinin systems in the development of cardiovascular disease in athletes has not been defined. This study aims to determine the polymorphisms of four genes (ACE, BDKRB2, PPARGC1A and NOS3) and the total genetic score to reveal the predisposition to the formation of physical qualities in martial arts athletes with different athletic abilities. The products of these four genes are involved in the control of blood pressure. The allelic variants of these genes are associated with the development of the physical quality “endurance” and have an indirect influence on the formation of speed and power qualities. The total genetic score (TGS: from 0 to 100 arbitrary units) was calculated from the genotype score in each polymorphism. The athletes were divided into Group I with high and Group II with low qualifications depending on their sports success. Single nucleotide polymorphisms (SNPs) are identified through restriction endonucleases cleavage for PCR amplicons for discriminating between alleles of the target genes ACE (rs4646994), BDKRB2 (rs5810761), PPARGC1A (rs8192673) and NOS3 (rs1799983). Significant differences between the allelic variants of target genes and athletic ability were found between Group I and Group II for genotype G/G of NOS3 gene and genotypes Gly/Gly and Gly/Ser of PPARGC1A gene. The data obtained confirm that athletes with unfavourable genotypes are excluded in the screening phase because their endurance is not fully developed to the required level in martial arts. Martial arts athletes with the highest TGS have the highest skill level. Polymorphic loci of four genes whose products are involved in blood pressure control (ACE, BDKRB2, NOS3 and PPARGC1A) can be used in martial arts not only to determine predisposition to cardiovascular disease but also to predispose to the development of speed and power qualities and endurance. The total genetic score can serve as a tool for predicting athletic success.
Shifting the Focus: A Pilot Study on the Effects of Positive Body Exposure on Body Satisfaction, Body Attitude, Eating Pathology and Depressive Symptoms in Female Patients with Eating Disorders
Authors: Rekkers Marlies E. , Aardenburg Lisanne , Scheffers Mia , van Elburg Annemarie A. , van Busschbach Jooske T.
Published: September 2022

One of the most commonly used techniques for the treatment of body image problems in eating disorders (ED) is body exposure (BE). However, evidence of its effectiveness in clinical populations is scarce. In the Positive Body Experience (PBE) protocol, the focus of positive BE is on aesthetic, functional and tactile aspects of the body. The current study evaluates the outcomes of positive BE with regard to changes in attitudinal body image and eating pathology, as well as the factors that influence these changes, in a sample of 84 adult female patients with different EDs who did not receive any other treatment for their EDs during the period in which BE treatment occurred. The results show significant positive changes in attitudinal body image, ED behaviors and depressive symptoms, with depressive symptoms at baseline mediating the changes in attitudinal body image. This study indicates that the PBE protocol is a suitable intervention for reducing negative attitudinal body image in anorexia and bulimia nervosa patients, as well as those with binge eating disorder. Furthermore, the results suggest that positive non-weight-related and functional body satisfaction are strong catalysts for change and that depressive symptoms play an important role in the ability to change. Additional RCTs are needed to gain more insight into the effects of PBE.
The Effect of Short Treatment with Nigella Sativa on Symptoms, the Cluster of Differentiation (CD) Profile, and Inflammatory Markers in Mild COVID-19 Patients: A Randomized, Double-Blind Controlled Trial
Authors: Bin Abdulrahman Khalid , Bamosa Abdullah , Bukhari Abdullah , Siddiqui Intisar , Arafa Mostafa , Mohsin Ashfaq , Althageel Mamdouh , Aljuaeed Majed , Aldeailej Ibrahim , Alrajeh Abdulaziz , Aldosari Kamel , Hawsawi Najat , Zawbaee Khalid , Alsurayea Saad
Published: September 2022

The current study investigated the impact of different doses of Nigella sativa seeds on the symptoms, the cluster of differentiation profile group, and inflammatory markers of mild COVID-19 cases. Methods: The study was a double-blind placebo-controlled clinical trial. Patients with mild and asymptomatic SARS-CoV-2 infection patients were randomly subdivided into seven subgroups: Group (GP) 1: received charcoal capsules as a control group, and GP 2: received three capsules of whole Nigella sativa seeds daily, two capsules in the morning and one in the evening; GP 3: received three capsules of whole Nigella sativa seeds every 12 h, GP 4: received five capsules in the morning and four capsules of whole Nigella sativa seeds in the evening, GP 5: received one capsule of Nigella sativa powder every 12 h; GP 6: received two capsules of Nigella sativa powder every 12 h; GP 7: received three capsules of Nigella sativa powder every 12 h; all treatment course was for ten days. Inflammatory parameters were assessed before and after interventions. Results: 262 subjects were included in the final analysis. No significant difference was detected regarding age, gender, and nationality. No significant differences were detected between the inflammatory marker in all groups. The WBCs showed a significant difference between before and after the intervention. While for procalcitonin, a significant difference was demonstrated in groups 1,4, and 6. Conclusions: The current randomized clinical trial did not reveal a significant effect of ten days of treatment with various doses of Nigella sativa on symptoms, differentiation profile, and inflammatory markers of patients with COVID-19. As a natural product, the effect of Nigella sativa on disease requires weeks to manifest itself.