bluuebunny/crossref_metadata_2025 huggingface dataset:
Bilinguismo na China
Authors: Ye Li
Published: January 2022

Bilinguismo é um fenômeno bastante comum no mundo inteiro e se apresenta sob diversas formas. O presente trabalho visa apresentar o caso da China. A China antigamente era constituída por diversos povos, cada um falante de uma língua distinta. Para facilitar a comunicação e manter a união do país, o governo adotou o mandarim como língua oficial da China. Ela é uma língua moderna e simplificada criada com base no dialeto de Pequim. Apesar da criação da língua oficial, as línguas dos povos antigos continuam presentes nas diversas regiões da China, constituindo dialetos completamente diferentes do mandarim na forma fonética. A divulgação do mandarim para a quase totalidade da população se deu principalmente através da educação e da mídia, queobrigatoriamente têm que utilizar somente o mandarim. Ao longo do trabalho são apresentadas a educação do mandarim, sua contribuição para o desenvolvimento do país, as consequências da obrigatoriedade do uso do mandarim, a educação bilíngue que existe em algumas regiões da China e o fenômeno bilíngue decorrente do fato de muitos chineses falarem duas línguas, o mandarim e o dialeto local, alternando entre elas em diferentes contextos.
Distribuição espacial e espaço temporal de óbitos de mulheres vivendo com HIV em Porto Alegre de 2007 a 2017
Authors: Bernardelli Maiton , Gonçalves Tonantzin Ribeiro , Pattussi Marcos Pascoal , Barcellos Nêmora Tregnago , Acosta Lisiane
Published: January 2022

OBJETIVO: Apresentar fatores associados à taxa de óbitos de mulheres vivendo com HIV (MVHIV) na cidade de Porto Alegre-RS. MÉTODOS: Estudo ecológico de análise espacial e espaço temporal incluindo todas as mulheres acompanhadas nos serviços de atenção à transmissão vertical (TV) do HIV, entre 2007 e 2017, residentes no município e que faleceram nesse período. As unidades de análise foram os 17 distritos sanitários do município. A variável dependente foi a taxa de óbito e as independentes territoriais foram os indicadores de vulnerabilidade à pobreza, proporção de mulheres chefe de família, escassez de infraestrutura, IDH e o índice de GINI, já as advindas da informação individual foram: idade, raça/cor, escolaridade, tempo de diagnóstico HIV. As análises utilizaram o SPSS 20.0 e o QGIS 218.15. RESULTADOS: Taxas mais elevadas de óbito de MVHIV foram registradas nas regiões com maior vulnerabilidade à pobreza e precariedade na infraestrutura local, associadas ao desfecho em mulheres em idade fértil, pretas/pardas e de baixa escolaridade. Nas regiões com altas taxas de mulheres que chefiam a família, a prevalência de óbito foi sete vezes maior, mesmo resultado obtido com a proporção populacional de vulnerabilidade à pobreza. CONCLUSÕES: As regiões que apresentam indicadores de vulnerabilidade críticos resultaram em taxas mais elevadas de óbito em MVHIV, revelando o impacto das desigualdades sociais em saúde para a morte dessas mulheres.
Single-Cell Transcriptome Analysis Reveals Embryonic Endothelial Heterogeneity at Spatiotemporal Level and Multifunctions of MicroRNA-126 in Mice
Authors: (郭 方浩)  , (关 亚娜)  , (郭 君君)  , (张 陆军)  , (邱 京晶)  , (季 勇)  , (陈 丰原)  , (荆 清)
Published: January 2022

Background:

Endothelial cells (ECs) play a critical role in angiogenesis and vascular remodeling. The heterogeneity of ECs has been reported at adult stages, yet it has not been fully investigated. This study aims to assess the transcriptional heterogeneity of developmental ECs at spatiotemporal level and to reveal the changes of embryonic ECs clustering when endothelium-enriched microRNA-126 (miR-126) was specifically knocked out.

Methods:

C57BL/6J mice embryos at day 14.5 were harvested and digested, followed by fluorescence-activated cell sorting to enrich ECs. Then, single-cell RNA sequencing was applied to enriched embryonic ECs. Tie2 (Tek receptor tyrosine kinase)-cre–mediated ECs-specific miR-126 knockout mice were constructed, and ECs from Tie2-cre–mediated ECs-specific miR-126 knockout embryos were subjected to single-cell RNA sequencing.

Results:

Embryonic ECs were clustered into 11 groups corresponding to anatomic characteristics. The vascular bed (arteries, capillaries, veins, lymphatics) exhibited transcriptomic similarity across the developmental stage. Embryonic ECs had higher proliferative potential than adult ECs. Integrating analysis showed that 3 ECs populations (hepatic, mesenchymal transition, and pulmonary ECs) were apparently disorganized after miR-126 being knocked out. Gene ontology analysis revealed that disrupted ECs were mainly related to hypoxia, glycometabolism, and vascular calcification. Additionally, in vivo experiment showed that Tie2-cre–mediated ECs-specific miR-126 knockout mice exhibited excessive intussusceptive angiogenesis; reductive glucose and pyruvate tolerance; and excessive accumulation of calcium. Agonist miR-126-3p agomir significantly rescued the phenotype of glucose metabolic dysfunction in Tie2-cre–mediated ECs-specific miR-126 knockout mice.

Conclusions:

The heterogeneity of ECs is established as early as the embryonic stage. The deficiency of miR-126 disrupts the differentiation and diversification of embryonic ECs, suggesting that miR-126 plays an essential role in the maintenance of ECs heterogeneity.
Acute Carotid Artery Stenting Versus Balloon Angioplasty for Tandem Occlusions: A Systematic Review and Meta‐Analysis
Authors: Zevallos Cynthia B. , Farooqui Mudassir , Quispe‐Orozco Darko , Mendez‐Ruiz Alan , Dajles Andres , Garg Aayushi , Galecio‐Castillo Milagros , Patterson Mary , Zaidat Osama , Ortega‐Gutierrez Santiago
Published: January 2022

Background

Despite thrombectomy having become the standard of care for large‐vessel occlusion strokes, acute endovascular management in tandem occlusions, especially of the cervical internal carotid artery lesion, remains uncertain. We aimed to compare efficacy and safety of acute carotid artery stenting to balloon angioplasty alone on treating the cervical lesion in tandem occlusions. Similarly, we aimed to explore those outcomes’ associations with technique approaches and use of thrombolysis.

Methods and Results

We performed a systematic review and meta‐analysis to compare functional outcomes (modified Rankin Scale), reperfusion, and symptomatic intracranial hemorrhage and 3‐month mortality. We explored the association of first approach (anterograde/retrograde) and use of thrombolysis with those outcomes as well. Two independent reviewers performed the screening, data extraction, and quality assessment. A random‐effects model was used for analysis. Thirty‐four studies were included in our systematic review and 9 in the meta‐analysis. Acute carotid artery stenting was associated with higher odds of modified Rankin Scale score ≤2 (odds ratio [OR], 1.95 [95% CI, 1.24–3.05]) and successful reperfusion (OR, 1.89 [95% CI, 1.26–2.83]), with no differences in mortality or symptomatic intracranial hemorrhage rates. Moreover, a retrograde approach was significantly associated with modified Rankin Scale score ≤2 (OR, 1.72 [95% CI, 1.05–2.83]), and no differences were found on thrombolysis status.

Conclusions

Carotid artery stenting and a retrograde approach had higher odds of successful reperfusion and good functional outcomes at 3 months than balloon angioplasty and an anterograde approach, respectively, in patients with tandem occlusions. A randomized controlled trial comparing these techniques with structured antithrombotic regimens and safety outcomes will offer definitive guidance in the optimal management of this complex disease.
Reducing Morbidity and Mortality in Patients With Coarctation Requires Systematic Differentiation of Impacts of Mixed Valvular Disease on Coarctation Hemodynamics
Authors: Sadeghi Reza , Tomka Benjamin , Khodaei Seyedvahid , Garcia Julio , Ganame Javier , Keshavarz‐Motamed Zahra
Published: January 2022

Background

Despite ongoing advances in surgical techniques for coarctation of the aorta (COA) repair, the long‐term results are not always benign. Associated mixed valvular diseases (various combinations of aortic and mitral valvular pathologies) are responsible for considerable postoperative morbidity and mortality. We investigated the impact of COA and mixed valvular diseases on hemodynamics.

Methods and Results

We developed a patient‐specific computational framework. Our results demonstrate that mixed valvular diseases interact with COA fluid dynamics and contribute to speed up the progression of the disease by amplifying the irregular flow patterns downstream of COA (local) and exacerbating the left ventricular function (global) (N=26). Velocity downstream of COA with aortic regurgitation alone was increased, and the situation got worse when COA and aortic regurgitation coexisted with mitral regurgitation (COA with normal valves: 5.27 m/s, COA with only aortic regurgitation: 8.8 m/s, COA with aortic and mitral regurgitation: 9.36 m/s; patient 2). Workload in these patients was increased because of the presence of aortic stenosis alone, aortic regurgitation alone, mitral regurgitation alone, and when they coexisted (COA with normal valves: 1.0617 J; COA with only aortic stenosis: 1.225 J; COA with only aortic regurgitation: 1.6512 J; COA with only mitral regurgitation: 1.3599 J; patient 1).

Conclusions

Not only the severity of COA, but also the presence and the severity of mixed valvular disease should be considered in the evaluation of risks in patients. The results suggest that more aggressive surgical approaches may be required, because regularly chosen current surgical techniques may not be optimal for such patients.
Effects of an Agro-healing Program on Promoting Mental Health of the Middle-aged
Authors: Kim Jae Soon , Yoo Eunha , Jeong Sun-Jin , Jang Hye Sook
Published: January 2022

Background and objective This study was conducted with 10 men and women in their 50s-60s to investigate the effect of agro-healing activities on the improvement of mental health. Methods The experimental group participated in total 8 sessions of agro-healing activities, once a week for 2 hours each, at a care farm in Wanju-gun. Physiological measurements were taken with an electroencephalogram (EEG), Salivettes samples, and blood pressure before and after the activities. Results As a result of analyzing the changes in brainwaves of the experimental group before and after agro-healing activities, relative slow alpha (RSA), relative fast alpha (RFA), and ratio of alpha to high beta (RAHB), the indices of stability and relaxation, increased after the program with statistical significance. Also, the ratio of SMR to theta (RST) of the attention index increased on the right frontal lobes, temporal lobes, and left occipital lobes, and relative low beta (RLB) increasd on the frontal lobes, temporal lobes, and occipital lobes after the program with statistical significance. The sympathetic nervous system activity, which is a stress index, decreased after the program, whereas the parasympathetic nervous system activity, which is a relaxation index, increased, showing statistical significance (p < .05). As a result of analyzing the changes in blood pressure after the program, systolic blood pressure and diastolic blood pressure decreased from prehypertension to normal blood pressure, showing statistical significance. SThese results indicate that participating in agro-healing activities at a care farm for the 50–60s helps reduce stress and improve stability and relaxation as well as attention. Conclusion Thus, developing and applying customized agro-healing programs for participants will have a positive effect on brain activity and psychophysiological improvement by relieving tension and stress. However, there are limitations in generalizing the results of this study since most of agro-healing farms have low accessibility that leads to a low level of participants.
Development of Diameter Growth Models by Thinning Intensity of Planted Quercus glauca Thunb. Stands
Authors: Jung Su Young , Lee Kwang Soo , Kim Hyun Soo
Published: January 2022

Background and objective This study was conducted to develop diameter growth models for thinned Quercus glauca Thunb. (QGT) stands to inform production goals for treatment and provide the information necessary for the systematic management of this stands. Methods This study was conducted on QGT stands, of which initial thinning was completed in 2013 to develop a treatment system. To analyze the tree growth and trait response for each thinning treatment, forestry surveys were conducted in 2014 and 2021, and a one-way analysis of variance (ANOVA) was executed. In addition, non-linear least squares regression of the PROC NLIN procedure was used to develop an optimal diameter growth model. Results Based on growth and trait analyses, the height and height-to-diameter (H/D) ratio were not different according to treatment plot (p > .05). For the diameter of basal height (DBH), the heavy thinning (HT) treatment plot was significantly larger than the control plot (p < .05). As a result of the development of diameter growth models by treatment plot, the mean squared error (MSE) of the Gompertz polymorphic equation (control: 2.2381, light thinning: 0.8478, and heavy thinning: 0.8679) was the lowest in all treatment plots, and the Shapiro-Wilk statistic was found to follow a normal distribution (p > .95), so it was selected as an equation fit for the diameter growth model. Conclusion The findings of this study provide basic data for the systematic management of Quercus glauca Thunb. stands. It is necessary to construct permanent sample plots (PSP) that consider stand status, location conditions, and climatic environments.
Importance-Performance Analysis of Mountain Village Promotion Projects in the Forest Sector by Upper-Level Local Governments
Authors: Kang Byung-Hoon , Kim Seong-Hak , Chae Jin-Hae
Published: January 2022

Background and objective Due to the recent crisis of extinction in local areas, the mountain village promotion policy is recognized as an important task. This study examined the priorities of major policy projects in the forest sector that affect mountain village promotion. Methods For research methods, literature search, expert advisory meetings, and a survey were conducted. The survey was conducted on 42 policy stakeholders from June 1 to August 13, 2021. The literature search was based on policy projects in the forest sector by 8 upper-level local governments including mountain villages. For questionnaire items, 173 forest policy projects were classified into 27 types through expert review, and the importance and performance of each type were rated on a 5-point Likert scale. Paired t-test, IPA, Locus for Focus model, and Borich needs assessment were used as the analysis methods, and the statistical program SPSS 21.0 was used as the analytical tool. Results The results showed that ‘creating forest-related jobs’ and ‘supporting cultivation of professional forestry workers’ both showed high importance and performance, implying that they would show an effect in mountain village promotion. ‘Creating forests for environmental improvement’, ‘discovering forest cultural assets’ ‘establishing and boosting forest tourism’, ‘providing forest therapy services’, ‘creating forest-related jobs’, ‘supporting community revitalization’, and ‘urban-rural exchanges’ were found to be the types that needed improvement and concentration for mountain village promotion. In particular, ‘creating forests for environmental improvement' and ‘discovering forest cultural assets’ were derived as priority considerations for mountain village promotion policies. Conclusion In summary, it was found that in order to promote mountain villages, various content projects must be improved and carried out to enhance the physical environment and revitalize mountain villages.
Decrees for the “Volunteers” of the People
Authors: Elliff Bryan
Published: January 2022

The Damascus Document’s Pesher of the Well (CD 6:2–11) has generally been treated as an isolated unit, either as an example of Qumran exegesis or as evidence for the history of the sect. The present study offers a fresh reading of this section that gives special attention to its rhetorical function within the document and its relationship to the document’s legal material in particular. It is argued that the pesher was intended to authorize the body of legal rulings found within the document by interpreting the two lines of Numbers 21:18 as an outline of two stages of the sect’s history. The pesher is built around two anchor-words in the lemma: ‮שרים‬‎ (“officials”), a reference to the sect’s founders who established an authoritative body of torah rulings, and ‮נדיבי העם‬‎, a reference to the sect’s later “volunteer initiates” who were to remain faithful to these rules throughout the Epoch of Wickedness.
Excavations at the Iron Age Village Site of Fibobe II, Central Zambia
Authors: Goldstein Steven T. , Farr Jeremy , Kayuni Martha , Katongo Maggie , Fernandes Ricardo , Janzen Anneke , Markham Brooke , Kay Andrea , Crowther Alison , Boivin Nicole
Published: January 2022

The period from c. AD 900 to AD 1300 in southern Africa is characterized by transitions from small-scale Iron Age mixed economy communities to the beginnings of more intensive food production and eventually the emergence of complex polities. In Zambia, this coincides with the appearance of larger and more permanent agro-pastoralist villages that began participating in Indian Ocean trade networks. Unlike other parts of southern Africa where stone architecture became common, the predominance of wattle-and-daub type construction methods across Zambia have often impeded preservation of Iron Age activity areas. It has therefore been difficult to reconstruct how economic and land-use changes between the Early and Later Iron Ages impacted family and community relationships reflected in intra-site and intra-household spatial organization. Fibobe II, in the Mulungushi River Basin of Central Zambia, is a rare example of an Early-to-Mid Iron Age village site where these spatial patterns may be discernable due to preservation of activity spaces and vitrified remains of wattle-and-daub structures. This paper reports on new investigations following original testing of the site in 1979, confirming preservation of an Iron Age hut with distinct patterning of features, artifacts, and charcoal. These results reaffirm the unique nature of Fibobe II and indicate the potential for programs of household archaeology aimed at studying this important and understudied period in Zambian prehistory.
Patience, Suffering, and Tolerance: The Experience of Defeat and Exile among the Jesuits of Ethiopia (1632–59)
Authors: Cohen Leonardo
Published: January 2022

This article explores the last letters written by the Catholic patriarch of Ethiopia in exile Afonso Mendes, which illustrate that, in the face of defeat, Mendes has chosen to write the history of martyrdom, the sacrifice, and shedding of blood for the sake of faith. A group requires a sense of connection through a temporary axis. Mendes’s choice in these last years corresponds to the will of generating cohesion in space and continuity in time in a group that has confronted rupture, disillusionment, and deterioration. Mendes might have attempted to establish a framework that would allow him to alleviate the tension caused by the clash between the original aspirations and the flawed fulfillment of the objective. Therefore, the redaction of the processes of martyrdom and the creation of a calendar allows the transition into a place where a harmonious relationship between the past and the present is generated.
Martyrs and Missionaries: Strategies of Jesuit Sainthood between the Suppression and the Restoration
Authors: Motta Franco , Rai Eleonora
Published: January 2022

This article explores the promotion of “Jesuit sanctity,” in the delicate passage between the suppression and the restoration of the Society of Jesus, as a reflection of the process of revival of the order. The strategies of sainthood that were fostered by the ex-Jesuits during the suppression and by the restored Society reveal fundamental information about the self-image that the order wanted to show to the world. These strategies emerge clearly from the activity of the General Postulation for the Causes of Saints of the new Society of Jesus, which in the nineteenth century focused in particular on two models of sanctity: martyrs and missionaries (and often martyred missionaries). Presenting important case studies of Francesco De Geronimo and Andrzej Bobola, this article investigates the reasons why the Society of Jesus promoted these typologies of sanctity in lieu of the trauma of the suppression, which emerges as “martyrdom” in Jesuit sources, and in the process of re-establishment of the order. It eventually explores how this “policy” of sainthood fits more broadly in the history of the Catholic Church in the nineteenth and twentieth centuries.
A new replacement name for Hieracium atriglandulosum P.D. Sell (Cichorieae, Asteraceae)
Authors: IDREES MUHAMMAD , ZHANG ZHIYONG
Published: January 2022

N/A
Morphological and molecular evidence confirms a new species, Riccia subcrinita YouL.Xiang & R.L.Zhu) and Riccia junghuhniana Nees & Lindenb. (Ricciaceae, Marchantiophyta) new to China
Authors: XIANG YOU-LIANG , ZHANG ZHI-XIN , CHEN SHENG-WEN , YU JIAN-PING , HUANG WEN-ZHUAN , SHEN CHAO , ZHU RUI-LIANG
Published: January 2022

Riccia is the largest genus of complex thalloid liverworts (Marchantiopsida) with over 250 species currently accepted. Our recent investigation of Chinese liverworts found two interesting Riccia species, R. junghuhniana and Riccia subcrinita sp. nov. Riccia junghuhniana is currently known from Australia and Indonesia, while Riccia subcrinita is known only from China. Riccia subcrinita is similar to R. crinita, but differs in having smaller spores (56–71 µm in diameter), and spore distal surface and proximal surface alveoli without thick borders. The sequences of rbcL, rps4, and trnL-F, detailed descriptions, and illustrations of the Chinese specimens are provided. The range extension of R. junghuhniana suggests that more taxa of Riccia may have a wider distribution. The discovery of R. junghuhniana and the new species also suggests that a more intensive survey of Riccia diversity in China is necessary.
Two new species of the spider genus Sinamma Lin & Li, 2014 (Araneae, Tetrablemmidae) from Guangdong Province, China
Authors: CHENG WEIHUA , REN LINGSHU , TONG YANFENG , BIAN DONGJU , LI SHUQIANG
Published: January 2022

Two new species of the genus Sinamma Lin & Li, 2014, Sinamma quadrata Tong & Li, sp. nov. (male, female) and Sinamma yingae Tong & Li, sp. nov. (male, female) are described from Guangdong, China. The geographic distribution of the genus is updated and a key to all known species is provided.
A new species of sucking louse (Psocodea: Phthiraptera: Anoplura: Hoplopleuridae) from the pale field rat, Rattus tunneyi (Rodentia: Muridae), in Australia
Authors: WANG WEI , DURDEN LANCE A. , SHAO RENFU
Published: January 2022

We describe and illustrate a new species of sucking louse, Hoplopleura tunneya new species, from the Australian pale field rat, Rattus tunneyi Thomas (Rodentia: Muridae). Currently, 22 species of the genus Hoplopleura Enderlein, 1904 (Phthiraptera: Anoplura: Hoplopleuridae) are known from Australian endemic rodents. Among the seven “new endemic” rodent species of the genus Rattus in Australia, R. tunneyi is one of five hosts to Hoplopleura lice. In addition, we give a list of all the species of Hoplopleura known from Australian endemic rodents. Including the introduced species Polyplax spinulosa, the total number of sucking louse species known from Australian endemic rodents is now 24.
miR-339 Promotes the Recovery of the Bone Marrow Mesenchymal Stem Cells (BMSCs)-Induced Corneal Epithelium Damage
Authors: He Xiumei , Zhou Xiong , Feng Yueyue
Published: January 2022

This study intends to identify the expression profiles of micoRNAs during the recovery of damaged corneal epithelium induced by BMSCs. Differential expressions of miRNA after damage of corneal epithelium stimulated by BMSCs were analyzed based on micro-array and validated by qRT-PCR.
The miRNA’s effect on cell proliferative and apoptotic activity was evaluated through transfection of plasmid with over presentation of miRNA and inhibitor of miRNA. miR-339 was significantly down-regulated in the process of recovery of the damaged corneal epithelium induced by BMSCs.
Importin 13 and EGF expression was reduced after transfection of plasmid with over presentation of miR-339, which were reversed by transfection of the inhibitor of miR-339. Importin 13 was a target of miR-339. The cell proliferation and apoptosis could be restrained by miR-339 through regulation
of the expression of Importin 13. In conclusion, the damaged corneal epithelium induced by BMSCs could be recovered by miR-339 through restraining Importin 13 expression, indicating that it might be a novel target for amelioration of corneal epithelium damage.
Effects of Long Noncoding RNA TUG1 (Taurine Up-Regulated Gene 1) on Growth and Metastasis of the Non-Small Cell Lung Cancer and Its Mechanism
Authors: Liu Ming , Guo Shenghu , Cao Jing , Wu Zheng , Zhang Lei , Yang Chunwang , Niu Zhicheng , Wang Junyan
Published: January 2022

Objective: Our research was to discuss effects and mechanism of lncRNA TUG1 in NSCLC by vitro study. Methods: A549 and H1299 cells were divided into NC, pcDNA 3.1 and lncRNA TUG1 groups. Measuring cell proliferation using CCK-8 assay, cell apoptosis by flow cytometry,
invasion cell number by transwell and wound healing rate by wound healing assay. Relative gene and protein expressions by RT-qPCR and WB assay. Results: Compared with NC group, the cell proliferation rate, invasion cell number and wound healing rate were significantly depressed in A549
and H1299 cell lines (P < 0.001, respectively). By RT-qPCR and WB assay, lncRNA TUG1 gene expression were significantly increased (P < 0.001, respectively); E-cadherin gene and protein expression were significantly up-regulation, and N-cadherin and Vimentin gene and protein
expressions were significantly depressed compared with those of NC group in A549 and H1299 cell lines (P < 0.001, respectively). Conclusion: lncRNA TUG1 had effects to suppress NSCLC cell biological activities by regulation EMT relative gene and proteins expression in vivo
study.
Bone Marrow Stromal Cells Promotes Morphological Senescence of Prostate Cancer Cells and Inhibits Metastasis Associated 1 Gene Level
Authors: Yan Xuechuan , Wang Xin , Yang Haifeng , Yang Jian , Li Haikun
Published: January 2022

This study assessed the mechanism of Bone Marrow Stromal Cells (BMSCs) in prostate cancer (PC) and its effect on MTA-1 gene and PC cell senescence. PC-3 cells were assigned into QL group (prostate cancer group: normal culture) and GS group (BMSCs group: treated with BMSCs) followed
by analysis of MTA-1 level, cell senescence, apoptosis and invasion. MTA-1 level in QL group (0.83±0.07) was significantly higher than GS group (0.14±0.02) (P < 0.05), indicating that BMSCs had an inhibitory effect on MTA-1 expression. Similar change of MTA-l mRNA was
also found with higher level in QL group than GS group (P < 0.05). Cell senescence was found in QS group but not QL group, indicating that BMSCs promote cell senescence. Compared with GS group, QL group has a higher cell number in G0/G1 (67.13±6.45%) and S (19.59±3.35%)
than GS group (G0/G1:50.51±2.19% and S: 11.42±1.61%) but lower G2/M (QL: 15.97±3.59% versus GS: 32.25±3.24%). QL group had significantly lower cell apoptosis rate at 35 h (5.21±1.2%) and 45 h (3.97±0.95%) than GS group at 35 h (17.85±1.23%),
45 h (10.21±1.26%) with elevated number of invasions. In conclusion, BMSCs promote PC-3 cell senescence and apoptosis by inhibiting the expression of MTA-1 and reduce cell invasion ability.
Long Non-Coding RNA CASC7 Promotes Proliferation and Inhibits Apoptosis of Hepatocellular Carcinoma Cells via Downregulating miR-340-5p CASC7/miR-340-5p Axis in Hepatocellular Carcinoma
Authors: Liu Shengyong , Li Xiangcheng
Published: January 2022

Background: Hepatocellular carcinoma (HCC) is a common malignant tumor worldwide with a poor prognosis. Amounting studies revealed that long non-coding RNAs (lncRNAs) show important roles in various biological processes. The purpose of this study was to explore the biological
function and potential molecular mechanism of CASC7 in HCC. Methods: CASC7 expression in HCC cell lines was detected by qRT-PCR. The expressions of CASC7 and miR-340-5p were changed by transfection of miR-340-5p mimic, the CASC7 overexpression and knockdown plasmids. The interaction
between CASC7 and miR-340-5p was assessed by a Dual-Luciferase reporter assay. The biological functions of CASC7 were evaluated by CCK-8, colony formation assay, ROS assay kit, immunofluorescence and flow cytometry (FCM). Results: CASC7 was upregulated in HCC cell lines. CASC7 overexpression
significantly promoted cell proliferation, as well as inhibited apoptosis and oxidative stress. In contrast, CASC7 knockdown could reverse these above changes. The result of the Dual-luciferase reporter assay revealed that CASC7 directly targeted miR-340-5p and negatively regulated its expression.
In addition, CASC7 promoted proliferation and inhibited apoptosis of HCC cells through activating Nrf2 pathway by downregulating miR-340-5p. Conclusions: In summary, CASC7 promotes HCC tumorigenesis and progression through the Nrf2 pathway by targeting miR-340-5p, which may provide
a new target for therapy of HCC.
Effects of Muscone on Cartilage Morphology and Matrix Metalloproteinases-3 (MMP-3h) and Matrix Metalloproteinases-9 (MMP-9) Expression in Rheumatoid Arthritis Rat Models
Authors: Zhong Xue , Jin Yuebo , Feng Yufei
Published: January 2022

Aim: To discuss Muscone treatment in Rheumatoid Arthritis Rat Models and relative mechanisms. Materials and methods: Dividing 36 rats as 4 groups as Normal, Model, DMSO and Muscone groups (n = 9). Rats of Model, DMSO and Muscone groups were made Rheumatoid Arthritis
model. Muscone group were treated with 2 mg/kg Muscone after modeling. HE staining and Masson staining were used to observe the morphological changes of cartilage tissue, measuring MMP-3 and MMP-9 expression by RT-PCR, Western Blotting (WB) and Immunohistochemistry (IHC). Results: Compared
with Model group, the pathological changes of Muscone group was significantly improved and average optical density of collagen fibers was significantly depressed (P < 0.001, respectively) via MMP-3 and MMP-9 proteins significantly depressing (P < 0.001, respectively). Conclusion:
Muscone improved Rheumatoid Arthritis by depressing MMP-3 and MMP-9 proteins in vivo study.
Protein–Protein Interactions Prediction Base on Multiple Information Fusion via Graph Representation Learning
Authors: Li Yan , Zhang Yu-Ren , Zhang Ping , Li Dong-Xu , Xiao Tian-Long
Published: January 2022

It is a critical impact on the processing of biological cells to protein–protein interactions (PPIs) in nature. Traditional PPIs predictive biological experiments consume a lot of human and material costs and time. Therefore, there is a great need to use computational methods to forecast PPIs. Most of the existing calculation methods are based on the sequence characteristics or internal structural characteristics of proteins, and most of them have the singleness of features. Therefore, we propose a novel method to predict PPIs base on multiple information fusion through graph representation learning. Specifically, firstly, the known protein sequences are calculated, and the properties of each protein are obtained by k-mer. Then, the known protein relationship pairs were constructed into an adjacency graph, and the graph representation learning method–graph convolution network was used to fuse the attributes of each protein with the graph structure information to obtain the features containing a variety of information. Finally, we put the multi-information features into the random forest classifier species for prediction and classification. Experimental results indicate that our method has high accuracy and AUC of 78.83% and 86.10%, respectively. In conclusion, our method has an excellent application prospect for predicting unknown PPIs.
miR-136 Suppresses the Aggressive Proliferation of Non-Small Cell Lung Cancer Through Restraining Histone Deacetylase 1 (HDAC1) and Phosphorylation of the Janus Kinase 2/Signal Transducer and Activator of Transcription 3 (Jak2/STAT3) Pathway
Authors: Yu Liang , Zhang Sheng , He Wei
Published: January 2022

microRNA-136 can inhibit the proliferating activity of malignant cells and also participate in chemotherapy resistance of colorectal cancer via modulating HDAC1. This study assessed miR-136’s effect on NSCLC cell proliferation and underlying mechanisms. Tumor tissues and paracancerous
tissues from NSCLC patients were collected to measure miR-136 and HDAC1 level. Cells were transfected with miR-136-mimics, miR-136-inhibitors or miR-136 mimics+HDAC1-OE followed by analysis of cell viability and apoptosis by CCK-8 method and flow cytometry, phosphorylation of Jak2/STAT3 by
western blot. miR-136 was significantly downregulated in tumor tissues and NSCLC cells, accompanied by upregulated HDAC1. miR-136 overexpression suppressed HDAC1 expression, retarded phosphorylation and activation of Jak2/STAT3 signaling, reduced NSCLC cell viability and enhanced apoptosis.
In addition, co-transfection of miR-136-mimics and HDAC1-OE reversed the inhibitory effects of miR-136 on NSCLC cells. In conclusion, miR-136 is reduced and HDAC1 is increased in NSCLC and miR-136 overexpression inhibited NSCLC cell proliferation and increased apoptosis possibly through regulating
HDAC1/Jak2/STAT3 signal pathway, indicating that miR-136 might be a novel target for the treatment of NSCLC.
Nrf2 Improves Airway Goblet Cell Metaplasia in Chronic Obstructive Pulmonary Disease (COPD) and Its Mechanism
Authors: Qiu Zhihong , Yan Li , Xu Juan , Qian Xiaojun
Published: January 2022

Objective: The aim of our research was to evaluate Nrf2 in COPD treatment and relative mechanism by vivo study.Materials: The mice were divided into Normal, Model and CCL16 groups. Measuring Pathology and goblet cell number by HE or AB/PAS staining; Evaluating apoptosis cell number by TUNEL assay; using flow separation to analysis inflammatory cells in difference groups; MAPK and NF-κB(p65) protein expression were evaluated by IHC assay in tissues; Total protein concentration of MUC5AC, Nrf2, Bax and Bcl-2 were evaluated by WB assay.Results: Compared with Normal group, the pathology was deteriorate and goblet cell number were significantly up-regulation in Model group, apoptosis goblet cell number were significantly depressed (P< 0.001), lympbocyte rate and hypertrophic rate were significantly down-regulation and Eosinophils rate, Macrophage rate and Neutrophils rate were significantly up-regulation (P< 0.001, respectively) in Model group. By IHC assay, MAPK and NF-κB(p65) proteins expression significantly increased (P< 0.001, respectively) in Model group; by WB assay, MUC5AC and Bcl-2 protein expression were significantly up-regulation and Nrf2 and Bax proteins expression were significantly down-regulation (P< 0.001, respectively) in Model group. Nrf2 supplement, the COPD were significantly improved with relative inflammatory cells rates significantly improving and relative proteins improving.Conclusion: Nrf2 could improve COPD by inducing goblet cell apoptosis increasing via regulation MAPK/NF-κB(p65) pathwayin vivostudy.
Knockdown of has_circ_0010452 Promotes Proliferation and Osteogenic Differentiation via Up-Regulating miR-543 in Human Bone Marrow Mesenchymal Stem Cells
Authors: Chen Siyuan , Guo Weixiong , Wei Jinsong , Lin Han , Guo Fengyan
Published: January 2022

Objective: The aim of this study was to explore the role of has_circ_0010452 in the progression of osteoporosis (OP) targeting miR-543, as well as their functions in regulating proliferation and osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).
Methods: The expression levels of circ_0010452 and miR-543 in hBMSCs at different time points of osteogenic differentiation were determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). After transfection of circ_0010452 siRNA or miR-543 inhibitor in hBMSCs, the relative
expression levels of osteogenic marker proteins, including oat spelt xylan (OSX), osteocalcin (OCN) and collagen I (Col-1), were determined by western blot. Cell proliferation of hBMSCs was valued by Cell Counting Kit 8 (CCK-8) assay. Dual-Luciferase reporter gene assay was performed to verify
the relationship between circ_0010452 and miR-543. Subsequently, the regulatory effects of circ_0010452 and miR-543 on osteogenic differentiation and the capability of mineralization were evaluated by alkaline phosphatase (ALP) determination and alizarin red staining, respectively. Results:
The expression of circ_0010452 decreased gradually and miR-543 increased in hBMSCs with the prolongation of osteogenic differentiation. circ_0010452 could bind to miR-543, which was negatively regulated by miR-543 in hBMSCs. Moreover, knockdown of circ_0010452 inhibited proliferation and osteogenic
differentiation by upregulating miR-543, as well as upregulating expressions of OSX, OCN and Col-1. Furthermore, knockdown of circ_0010452 markedly promoted the capability of mineralization of hBMSCs, which was further reversed by transfection of miR-543 inhibitor. The knockdown of miR-543
partially reversed the inhibitory effect of circ_0010452 on the osteogenesis of hBMSCs. Conclusions: Silence of circ_0010452 promotes the development of OP via binding to miR-543 regulating proliferation and osteogenic differentiation of hBMSCs, thus promoting the progression of osteoporosis.
miR-12 Derived from Bone Marrow Mesenchymal Stem Cells Accelerates the Development of Human Papillomavirus by Up-Regulating AN1
Authors: Mijiti Yilidana , Fang Fang , Liang Shanhui , Huang Xiuju , Yilihamu Yilidana , Zhakeer Adilai , Wang Jing
Published: January 2022

The miRNA derived from Bone marrow mesenchymal stem cells (BMSCs) have crucial effects on tumors. The tumor could be affected by the abnormal expression of miRNA in human papillomavirus (HPV). Our study aimed to identify the potential brand-new biomarker in order to reveal the pathogenesis
of HPV. miRNA derived from BMSCs was detected and identified. The action of miR-12 on biological behavior of HPV was detected. The level of AN1 protein was detected by Western-blot and IHC method. The relationship between miR-12 and AN1 was assessed by bioinformatics analysis and luciferase
assay. The tumor cell biological behaviors were evaluated by manipulating miR12 and AN1 level. The tumor volume derived from BMSCs was diminished significantly compared with normal tissues. The tumor volume was bigger after combined injection with Hela cell and miR-12 compared with single
injection. The cell proliferative and invasive ability was strengthened after transfection with miR-12mimics. The cell invasive ability was reduced significantly after transfection of si-miR-12. AN1 was a target gene of miR-12 as confirmed by the analysis on bioinformatics and luciferase activity.
The phenotype was reversed after the silent presentation of AN1 was disturbed. In conclusion, miR-12 expression is elevated in HPV cells and affects HPV cells through targeting the AN1 signaling pathway.
Effect of Krüppel-Like Factor 7 (KLF7) on High Sugar Induced Retinal Ganglion Cell Biological Activity and Oxidative Stress
Authors: Liu Lan , Cheng Xinchao , Li Shaomin
Published: January 2022

This study investigated KLF7’s effect on sugar induced retinal ganglion cells (RGCs) biological activity. The RGCs cells divided into blank group (RA), high sugar group (RB), high sugar+NC group (RC) and high sugar+KLF7 group (RD) (transfected with KLF7 mimic) followed by analysis
cell proliferation by MTT, cell apoptosis by flow cytometry and protein expression by western blot and ROS level. RB and RC group showed significantly reduced KLF7 mRNA and protein level compared to RA group (P < 0.05) without different between RB and RC group (P > 0.05).
RD group had significantly increased LKF7 and Sirt1 protein expression (F = 113.3, P < 0.0, 01), reduced cell proliferation (P < 0.05) and increased RGCs apoptosis rate (P < 0.05) compared with RB and RC group. After 24 h, RB and RC group presented significantly
higher ROS level (P < 0.05) which was reduced in RD group (P < 0.05). In conclusion, KLF7 can change sugar induced retinal ganglion cell biological activity and reduce the oxidative stress level.
Bone Marrow Mesenchymal Stem Cells (BMSCs) with a Disintegrin and Metalloprotease 17 (ADAM17) Overexpression Increase Cervical Cancer Cell Proliferation and Migration Through Epidermal Growth Factor Receptor (EGFR)/Phosphatidylinositol 3-Kinase (PI3K)/Protein Kinase b (AKT) Signaling
Authors: Gao Li , Lv Shulan , Zhu Yan
Published: January 2022

ADAM-17 is a membrane-bound protease and highly expressed in multiple tumors. BMSCs carrying target genes are delivered to damaged sites. This study aimed to investigate the mechanism underlying BMSCs with ADAM-17 in cervical cancer (CC). BMSCs were transfected with ADAM-17 mimics and
co-cultured with CC cells followed by analysis of cell proliferation and migration by MTT assay and scratch assay, ADAM-17 and target genes (LAMB3, Robol) level by Western blot and RT-qPCR. As the effectiveness of ADAM-17 transfection was confirmed by its increased level, the presence of empty
vector rarely affected ADAM-17 expression and biological activities of CC cells compared to control group (p > 0.05). BMSCs with ADAM-17 overexpression increased CC cell proliferation and enhanced scratch healing rate (p < 0.05), accompanied with upregulated LAMB3 and Robol.
The difference in LAMB3 and Robol expression between empty vector group and control group did not reach a significance. In conclsuion, this study elucidates that BMSCs with ADAM-17 overexpression promotes CC cell progression through up-regulation of LAMB3 and Robol and activation EGFR/PI3K/Akt
signaling, providing a novel BMSC-based targeted therapy.
Bone Marrow Mesenchymal Stem Cells (BMSCs)-Exosome Inhibits Epithelial Ovarian Cancer (EOC) Cell Proliferative Ability Through Regulating Mitogen-Activated Protein Kinase (MKP)-1 and Mitogen-Activated Protein Kinases (MAPK)/Extracellular-Signal-Regulated Kinase (ERK) Signal Pathway
Authors: Li Qian , Wang Ting , Shen Yang , Du Juan
Published: January 2022

The BMSCs-exosome plays a role in regulating tumor micro-environment so as to affect tumor cell biological behaviors. However, whether it affects the biological characteristics of epithelial ovarian cancer (EOC) cells remains unclear. Our study aimed to discuss whether BMSCs-exosome
affects EOC cell proliferative ability. BMSCs cells were cultivated to isolate exosome which was used to treat EOC cells at different concentrations (25, 50, and 100 μmol/L) followed by measuring cell proliferation by CCK-8, cell invasion and migration by Transwell, MKP-1and MAPK/ERK
protein level by Western Blot. BMSCs-exosome showed positive expression of CD9, CD63 and CD81 and negative CD116 and CD19. It could significantly inhibit EOC cell proliferation, invasion and migration in a dose-dependent manner along with reduced expression of MAPK/ERK. In conclusion, BMSCs-exosome
inhibits EOC cell biological behaviors possibly through regulation of MKP-1 and MAPK/ERK signal pathway, indicating that it might be used as a novel approach for treating EOC.
Effect of Remnant-Preserving Reconstruction of Acute Anterior Cruciate Ligament Injuries in a Rabbit Model: Histological and Biomechanical Analysis
Authors: Ju XiaoChen , Chai Hao , Krishnan Sasirekha , Jaisankar Abinaya , Ramalingam Murugan , Zhang Lei
Published: January 2022

Acute anterior cruciate ligament (ACL) is a key structure that stabilizes knee joints. The objective of this research is to investigate the influence of ligament remnants preserved on the tendon-bone healing following ACL reconstruction and to examine postoperative articular cartilage
degeneration in rabbit as a model animal. Sixty New Zealand rabbits are randomly divided into an ACL reconstruction without remnant preservation group (Group A; n = 30) or ACL reconstruction with remnant preservation group (Group B; n = 30). The expression of HIF-1α,
VEGF, and micro vessel density (MVD) in the transplanted tendon was detected by immunohistochemical staining at week 6 and 12 after the operation. The signal intensity of the transplanted tendon was observed by MRI scanning, and the width of the bone tunnel was measured by CT scanning at week
6 and 12 after the operation. The graft biomechanics was tested 12 weeks after the operation. The JNK and MMP-13 expression levels were compared to analyze the cartilage degeneration of the knee at week 12 after the operation. The experimental results were analyzed and showed that the remnant-preserving
ACL reconstruction is beneficial for bone healing of the tendon in rabbits, but ACL reconstruction with or without ligament remnants preserved will not affect knee articular cartilage degeneration post-surgery.
A systematic review of evidence-based aftercare for older adults following self-harm
Authors: Wand Anne PF , Browne Roisin , Jessop Tiffany , Peisah Carmelle
Published: January 2022

Objective:

Self-harm is closely associated with suicide in older adults and may provide opportunity to intervene to prevent suicide. This study aimed to systematically review recent evidence for three components of aftercare for older adults: (1) referral pathways, (2) assessment tools and safety planning approaches and (3) engagement and intervention strategies.

Methods:

Databases PubMed, Medline, PsychINFO, Embase and CINAHL were searched from January 2010 to 10 July 2021 by two reviewers. Empirical studies reporting aftercare interventions for older adults (aged 60+) following self-harm (including with suicidal intent) were included. Full text of articles with abstracts meeting inclusion criteria were obtained and independently reviewed by three authors to determine final studies for review. Two reviewers extracted data and assessed level of evidence (Oxford) and quality ratings (Alberta Heritage Foundation for Medical Research Standard Quality Assessment Criteria for quantitative and Attree and Milton checklist for qualitative studies), working independently.

Results:

Twenty studies were reviewed (15 quantitative; 5 qualitative). Levels of evidence were low (3, 4), and quality ratings of quantitative studies variable, although qualitative studies rated highly. Most studies of referral pathways were observational and demonstrated marked variation with no clear guidelines or imperatives for community psychiatric follow-up. Of four screening tools evaluated, three were suicide-specific and one screened for depression. An evidence-informed approach to safety planning was described using cases. Strategies for aftercare engagement and intervention included two multifaceted approaches, psychotherapy and qualitative insights from older people who self-harmed, carers and clinicians. The qualitative studies identified targets for improved aftercare engagement, focused on individual context, experiences and needs.

Conclusion:

Dedicated older-adult aftercare interventions with a multifaceted, assertive follow-up approach accompanied by systemic change show promise but require further evaluation. Research is needed to explore the utility of needs assessment compared to screening and evaluate efficacy of safety planning and psychotherapeutic approaches.