bluuebunny/crossref_metadata_2025 huggingface dataset:
The Evolution of Nitric Oxide Function: From Reactivity in the Prebiotic Earth to Examples of Biological Roles and Therapeutic Applications
Authors: Shepherd Mark , Giordano Daniela , Verde Cinzia , Poole Robert
Published: June 2022

Nitric oxide was once considered to be of marginal interest to the biological sciences and medicine; however, there is now wide recognition, but not yet a comprehensive understanding, of its functions and effects. NO is a reactive, toxic free radical with numerous biological targets, especially metal ions. However, NO and its reaction products also play key roles as reductant and oxidant in biological redox processes, in signal transduction, immunity and infection, as well as other roles. Consequently, it can be sensed, metabolized and modified in biological systems. Here, we present a brief overview of the chemistry and biology of NO—in particular, its origins in geological time and in contemporary biology, its toxic consequences and its critical biological functions. Given that NO, with its intrinsic reactivity, appeared in the early Earth’s atmosphere before the evolution of complex lifeforms, we speculate that the potential for toxicity preceded biological function. To examine this hypothesis, we consider the nature of non-biological and biological targets of NO, the evolution of biological mechanisms for NO detoxification, and how living organisms generate this multifunctional gas.
The Role of Antioxidants in the Interplay between Oxidative Stress and Senescence
Authors: Varesi Angelica , Chirumbolo Salvatore , Campagnoli Lucrezia Irene Maria , Pierella Elisa , Piccini Gaia Bavestrello , Carrara Adelaide , Ricevuti Giovanni , Scassellati Catia , Bonvicini Cristian , Pascale Alessia
Published: June 2022

Cellular senescence is an irreversible state of cell cycle arrest occurring in response to stressful stimuli, such as telomere attrition, DNA damage, reactive oxygen species, and oncogenic proteins. Although beneficial and protective in several physiological processes, an excessive senescent cell burden has been involved in various pathological conditions including aging, tissue dysfunction and chronic diseases. Oxidative stress (OS) can drive senescence due to a loss of balance between pro-oxidant stimuli and antioxidant defences. Therefore, the identification and characterization of antioxidant compounds capable of preventing or counteracting the senescent phenotype is of major interest. However, despite the considerable number of studies, a comprehensive overview of the main antioxidant molecules capable of counteracting OS-induced senescence is still lacking. Here, besides a brief description of the molecular mechanisms implicated in OS-mediated aging, we review and discuss the role of enzymes, mitochondria-targeting compounds, vitamins, carotenoids, organosulfur compounds, nitrogen non-protein molecules, minerals, flavonoids, and non-flavonoids as antioxidant compounds with an anti-aging potential, therefore offering insights into innovative lifespan-extending approaches.
Potential Neurotoxic Effects of Glioblastoma-Derived Exosomes in Primary Cultures of Cerebellar Neurons via Oxidant Stress and Glutathione Depletion
Authors: Genc Sidika , Pennisi Manuela , Yeni Yesim , Yildirim Serkan , Gattuso Giuseppe , Altinoz Meric A. , Taghizadehghalehjoughi Ali , Bolat Ismail , Tsatsakis Aristidis , Hacımüftüoğlu Ahmet , Falzone Luca
Published: June 2022

High-grade gliomas are the most fatal brain tumors. Grade 4 gliomas are called glioblastoma multiforme (GBM), which are associated with the poorest survival and a 5-year survival rate of less than 4%. Many patients with GBM developed concomitant cognitive dysfunctions and epilepsy. Although the cognitive decline is well defined in glioblastomas, the neurotoxic factors underlying this pathology are not well understood in GBM patients. In this study, we aimed to investigate whether GBM-derived exosomes play a role in neuronal toxicity. For this purpose, exosomes obtained from T98G and U373 GBM cells were applied to primary neuron culture at different concentrations. Subsequently, MTT, LDH, GSH, TAS, and TOS tests were performed. Both GBM-derived exosomes induced a dose-dependent and statistically significant increase of LDH release in cerebellar neurons. MTT assay revealed as both T98G and U373 GBM-derived exosomes induced dose-dependent neurotoxic effects in cerebellar neurons. To the best of our knowledge, this study is the first study demonstrating the toxic potential of GBM-derived exosomes to primary neurons, which may explain the peritumoral edema and cognitive decline in GBM patients.
Mask Branch Network: Weakly Supervised Branch Network with a Template Mask for Classifying Masses in 3D Automated Breast Ultrasound
Authors: Kim Daekyung , Park Haesol , Jang Mijung , Lee Kyong-Joon
Published: June 2022

Automated breast ultrasound (ABUS) is being rapidly utilized for screening and diagnosing breast cancer. Breast masses, including cancers shown in ABUS scans, often appear as irregular hypoechoic areas that are hard to distinguish from background shadings. We propose a novel branch network architecture incorporating segmentation information of masses in the training process. The branch network is integrated into neural network, providing the spatial attention effect. The branch network boosts the performance of existing classifiers, helping to learn meaningful features around the target breast mass. For the segmentation information, we leverage the existing radiology reports without additional labeling efforts. The reports, which is generated in medical image reading process, should include the characteristics of breast masses, such as shape and orientation, and a template mask can be created in a rule-based manner. Experimental results show that the proposed branch network with a template mask significantly improves the performance of existing classifiers. We also provide qualitative interpretation of the proposed method by visualizing the attention effect on target objects.
A Fully-Self-Adaptive Harmony Search GMDH-Type Neural Network Algorithm to Estimate Shear-Wave Velocity in Porous Media
Authors: Taheri Ahmad , Makarian Esmael , Manaman Navid Shad , Ju Heongkyu , Kim Tae-Hyung , Geem Zong Woo , RahimiZadeh Keyvan
Published: June 2022

Shear wave velocity (VS) is one of the most important parameters in deep and surface studies and the estimation of geotechnical design parameters. This parameter is widely utilized to determine permeability and porosity, lithology, rock mechanical parameters, and fracture assessment. However, measuring this important parameter is either impossible or difficult due to the challenges related to horizontal and deviation wells or the difficulty in reaching cores. Artificial Intelligence (AI) techniques, especially Machine Learning (ML), have emerged as efficient approaches for dealing with such challenges. Therefore, considering the advantage of the ML, the current research proposes a novel Fully-Self-Adaptive Harmony Search—Group Method of Data Handling (GMDH)-type neural network, named FSHS-GMDH, to estimate the VS parameter. In this way, the Harmony Memory Consideration Rate (HMCR) and Pitch Adjustment Rate (PAR) parameters are calculated automatically. A novel method is also introduced to adjust the value of the Bandwidth (BW) parameter based on the cosine wave and each decision variable values. In addition, a variable-size harmony memory is proposed to enhance both the diversification and intensification. Our proposed FSHS-GMDH algorithm quickly explores the problem space and exploits the best regions at the late iterations. This algorithm allows for the training of the prediction model based on the P-wave velocity (VP) and the bulk density of rock (RHOB). Applying the proposed algorithm to a carbonate petroleum reservoir in the Persian Gulf demonstrates that it is capable of accurately estimating the VS parameter better than state-of-the-art machine learning methods in terms of the coefficient of determination (R2), Mean Square Error (MSE), and Root Mean Square Error (RMSE).
Adaptive Sliding Mode Control via Backstepping for an Air-Breathing Hypersonic Vehicle Using a Double Power Reaching Law
Authors: Huang Shutong , Jiang Ju , Li Ouxun
Published: June 2022

This paper presents a backstepping-based adaptive sliding mode control scheme using a new double power reaching law for an air-breathing hypersonic vehicle (AHV) with uncertainties. A novel double power reaching law is proposed to speed up the state stabilization. A backstepping control scheme is proposed for a class of high-order nonlinear system with uncertainties. Then, a novel sliding mode controller using the new double power reaching law is developed to maintain the high tracking performance of the AHV. In order to further attenuate the influence of uncertainties, new adaptive laws are employed. Lastly, simulation studies show that the novel double power reaching law can guarantee that the state of the system converges to zero equilibrium in fixed time, and the controller proposed can effectively reduce the influence of uncertainties on the AHV and achieve good tracking performance.
Development of High-Fidelity Numerical Methodology for Prediction of Vehicle Interior Noise Due to External Flow Disturbances Using LES and Vibroacoustic Techniques
Authors: Lee Songjune , Lee Sang-heon , Cheong Cheolung
Published: June 2022

A pleasant and quiet cabin in driving a car is one of the most critical factors affecting a customer’s choice in a market. As the traditional noise sources such as power trains become less, the relative contribution of aerodynamic noise to the interior noise of a road vehicle becomes even more critical. In this study, a high-fidelity numerical methodology is developed for the reliable prediction and analysis of the interior transmitted noise due to external flow disturbance. The developed numerical methodology is based on the sequential application of the high-resolution LES technique, wavenumber–frequency transform, and vibroacoustic model. First, the compressible LES techniques with high-resolution grids are employed to accurately predict the external turbulent flow and aeroacoustic fields due to the turbulent flow, at the same time, of a vehicle running at a speed of 110 km/h. Second, surface pressure fluctuations on the front windshield and side windows, obtained from the LES simulation, are decomposed into incompressible and compressible ones using the wavenumber–frequency transform. Lastly, the interior sound pressure levels are predicted using the vibroacoustic model, which consists of the finite element (FE) and statistical energy analysis (SEA) methods. For the efficient computation of the vibroacoustic interaction between the vibration of the vehicle windows and the acoustic field inside the cabin room, the FE and SEA methods are applied in low- and high-frequency ranges, respectively. The predicted interior sound pressure spectral levels agree well with the measured ones. In addition, although the magnitudes of the compressible pressure components are generally lower than those of the incompressible ones, the compressible field is found to contribute more to the interior noise in high-frequency bands. The physical mechanism of the higher transmission is shown to be related to the coincident effect between the compressible pressure field and the structural vibration of the vehicle window.
A Control Method for the Differential Steering of Tracked Vehicles Driven Independently by a Dual Hydraulic Motor
Authors: Han Jiangyi , Wang Fan , Wang Yuhang
Published: June 2022

It is well known that tracked vehicles can adapt well to all kinds of terrain. However, the safety of tracked vehicles should be considered during steering on sloped terrain. This paper focuses on the differential steering control of tracked vehicles independently driven by a hydraulic motor. Firstly, the dynamic model of hydrostatic drive system was built and the kinematics and dynamics of differential steering driving were analyzed theoretically. Secondly, in order to prevent rollover of the tracked vehicle, the method of vehicle speed constraint was proposed. The constraint conditions of vehicle speed and steering angular velocity were analyzed under different slope conditions. Thirdly, based on the analysis results, differential steering control rules for tracked vehicles were formulated. To verify the effectiveness of the control rules, the models of vehicle driving dynamics and Fuzzy PID control simulation were established in MATLAB/Simulink. Longitudinal steering simulation was carried out on a slope (0°, 30°), and an analysis of the simulation of lateral steering along the contour line was carried out. The simulation results showed that this steering control strategy was able to automatically adjust the target vehicle speed to avoid rollover while the driver was inputting steering signals.
Performance Evaluation of Planetary Boundary Layer Schemes in Simulating Structures of Wintertime Lower Troposphere in Seoul Using One-Hour Interval Radiosonde Observation
Authors: Han Beom-Soon , Kwak Kyung-Hwan , Hahm Jae-Hee , Park Seung-Bu
Published: June 2022

We investigated the structures of the wintertime lower troposphere in Seoul, South Korea on 17 and 18 January 2017 by performing 1 h interval radiosonde observation and numerical simulations. In the daytime on 17 January, the height of the convective boundary layer (CBL) sharply and quickly increased when the residual layer became a part of the CBL. From the afternoon on 17 January, moist air with clouds began to substantially intrude in the lower troposphere in Seoul, and radiative heating/cooling weakened. As a result, the mixing of air in the lower troposphere was inhibited and the vertical gradients of potential temperature and water vapor mixing ratio changed little on 18 January. We evaluated the performance of four planetary boundary layer (PBL) parameterization schemes (the Yonsei University (YSU), Mellor–Yamada–Janjić (MYJ), Mellor–Yamada–Nakanishi–Niino (MYNN), and Asymmetric Convective Model version 2 (ACM2) schemes) coupled with the Weather Research and Forecasting model in simulating the structures of the lower troposphere against 1 h interval radiosonde observation. The general tendencies of the air temperature and wind speed in the lower troposphere were well-reproduced in the four simulations. However, the sharp increase in the CBL height did not appear in the four simulations, implying that the process of the residual layer becoming a part of the CBL in the daytime is not well-parameterized. Additionally, the simulated water vapor mixing ratio near the surface was smaller compared with the observation. We found that small-scale turbulence in the CBL, which mixes advected air and pre-existing air, was not reproduced well by the PBL parameterization schemes. Compared with the other simulations, the most accurate air temperature and wind speed were reproduced in the simulation with the MYJ scheme, while the CBL development and moisture advection were reproduced relatively well in the simulation with the MYNN scheme.
Construction and Application of Enterprise Knowledge Base for Product Innovation Design
Authors: Zhang Lulu , Tan Runhua , Peng Qingjin , Shao Peng , Dong Yafan , Wang Kang
Published: June 2022

As most of the knowledge used in industrial product design is based on data files from a previous design, it is difficult to be efficiently applied in supporting product innovation design. This paper proposes a method to construct an enterprise knowledge base (EKB) for product innovation design. A concept of the functional basis of product (FBP) is first proposed based on similar products. The function units and corresponding technical units are clustered to construct an EKB for product innovation design. A retrieval path of the knowledge is then proposed from the functional level. The prototype software is developed to retrieve the knowledge directly through function units and determine the optimal technology by searching and ranking relevant patents. The patent circumvention and Theory of Inventive Problem Solving (TRIZ) methods are used to solve invention problems and obtain innovative solutions. The built EKB model provides a systematic method for the innovative product design process. An underwater separator is developed in a case study to verify the proposed method.
Influence of Cognitive Task Difficulty in Postural Control and Hemodynamic Response in the Prefrontal Cortex during Static Postural Standing
Authors: Saraiva Marina , Paszkiel Szczepan , Vilas-Boas João Paulo , Castro Maria António
Published: June 2022

In daily life, we perform several tasks simultaneously, and it is essential to have adequate postural control to succeed. Furthermore, when performing two or more tasks concurrently, changes in postural oscillation are expected due to the competition for the attentional resources. The aim of this study was to evaluate and compare the center of pressure (CoP) behavior and the hemodynamic response of the prefrontal cortex during static postural standing while performing cognitive tasks of increasing levels of difficulty on a smartphone in young adults. Participants were 35 healthy young adults (mean age ± SD = 22.91 ± 3.84 years). Postural control was assessed by the CoP analysis (total excursion of the CoP (TOTEX CoP), displacements of the CoP in medial–lateral (CoP-ML) and anterior–posterior (CoP-AP) directions, mean total velocity displacement of CoP (MVELO CoP), mean displacement velocity of CoP in medial–lateral (MVELO CoP-ML) and anterior–posterior (MVELO CoP-AP) directions, and 95% confidence ellipse sway area (CEA)), the hemodynamic response by the oxyhemoglobin ([oxy-Hb]), deoxyhemoglobin ([deoxy-Hb]), and total hemoglobin ([total-Hb]) concentrations using a force plate and functional near-infrared spectroscopy (fNIR), respectively. The results showed that the difficult cognitive task while performing static postural standing caused an increase in all CoP variables in analysis (p < 0.05) and of [oxy-Hb] (p < 0.05), [deoxy-Hb] (p < 0.05) and [total-Hb] (p < 0.05) compared to the postural task. In conclusion, the increase in the cognitive demands negatively affected the performance of the postural task when performing them concurrently, compared to the postural task alone. The difficult cognitive task while performing the postural task presented a greater influence on postural sway and activation of the prefrontal cortex than the postural task and the easy cognitive task.
Spectroscopic Characterization of a Pulsed Low-Current High-Voltage Discharge Operated at Atmospheric Pressure
Authors: Szulc Michał , Forster Günter , Marques-Lopez Jose-Luis , Schein Jochen
Published: June 2022

The advantages of pulsed low-current high-voltage discharges operated at atmospheric pressure and the ease with which such discharges can be implemented industrially contributed to their popularity. However, the high reactivity of a pulsed plasma implies that thorough diagnostics are needed to fully understand the interactions inside these plasmas. Some of the key parameters determining plasma properties of low-current discharges are the electron number density and the temperature of heavy particles. Both parameters can be determined experimentally with spectroscopic techniques, for example by investigating the broadening of spectral lines due to the Stark effect and by fitting synthetic spectra to molecular transitions. To the authors’ knowledge, experimentally determined electron densities for pulsed low-current discharges operated in a power range between 300 W and 1000 W have not been performed in previous works. Thus, in this work, the electron number density and temperature of heavy particles of one of several commercially available plasma systems are determined by means of emission spectroscopy.
Frontiers in Atmospheric Pressure Plasma Technology
Authors: Nastuta Andrei Vasile
Published: June 2022

Atmospheric pressure plasmas represent a feasible and eco-friendly alternative to conventional physicochemical methods used in technology today for facing materials [...]
The Analysis of Pneumatic Wheel Rim Deformation While Hitting an Obstacle
Authors: Tarkowski Sławomir , Nieoczym Aleksander , Caban Jacek , Jilek Petr , Sejkorová Marie
Published: June 2022

The article presents the results of simulations using the finite element method (FEM) aimed at examining the extent of damage to the wheel rim as a result of hitting an obstacle. The obtained results can be used as comparative data during the performance of expert opinions to give an answer as to how the damage occurred. The data obtained from the FEM simulation can also be used in the process of geometric optimization of the rim, which aims to obtain a rim resistant to this type of damage.
Waterproof Design of Soft Multi-Directional Force Sensor for Underwater Robotic Applications
Authors: Cross Liam B. , Subad Rafsan Al Shafatul Islam , Saikot Md Mahmud Hasan , Park Kihan
Published: June 2022

Directional force sensing is an intrinsic feature of tactile sensing. As technologies of exploratory robots evolve, with special emphasis on the emergence of soft robotics, it is crucial to equip robotic end-effectors with effective means of characterizing trends in force detection and grasping phenomena, while these trends are largely derived from networks of tactile sensors working together, individual sensors must be built to meet an intended function and maintain functionality with respect to environmental operating conditions. The harshness of underwater exploration imposes a unique set of circumstances onto the design of tactile sensors. When exposed to underwater conditions a tactile sensor must be able to withstand the effects of increased pressure paired with water intrusion while maintaining computational and mechanical integrity. Robotic systems designed for the underwater environment often become expensive and cumbersome. This paper presents the design, fabrication, and performance of a low-cost, soft-material sensor capable of multi-directional force detection. The fundamental design consists of four piezo-resistive flex elements offset at 90∘ increments and encased inside of a hemispherical silicone membrane filled with a non-compressive and non-conductive fluid. The sensor is simulated numerically to characterize soft-material deformation and is experimentally interrogated with indentation equipment to investigate sensor-data patterns when subject to different contact forces. Furthermore, the sensor is subject to a cyclic loading test to analyze the effects of hysteresis in the silicone and is submerged underwater for a 7-day period to investigate any effect of water intrusion at a shallow depth. The outcome of this paper is the proposed design of a waterproofed, soft-material tactile sensor capable of directional force detection and contact force localization. The overall goal is to widen the scope of tactile sensor concepts outfitted for the underwater environment.
Coordinating Production and Delivery Schedule of Multi-Product and Multi-Customer through Mathematical Programming
Authors: Ai The Jin , Astanti Ririn Diar
Published: June 2022

Due to the shortage and high cost of shipping containers, manufacturers that distribute products for worldwide customers must optimize their delivery. Commonly, they complete their delivery schedule first before creating the production schedule. This article tries to address the scheduling problem arising in the process of creating the production schedule to meet the delivery schedule. The complexity of the situation is increased since there is concern about producing as few product types as possible in a single period and having a balance of production load over multiple periods. A mathematical programming model for addressing this production scheduling problem is proposed in the form of integer programming. This model can be solved using LINGO, a commercial mathematical programming software. Microsoft Excel® spreadsheets are used to store both the data input and the output of the mathematical programming model. The advantages of using Excel are its easy-to-read format and familiarity with most users in various companies. Computational experiments in several cases show that the proposed method is effective to create the production and delivery schedule in various problem settings as expected.
Efficacy of Kinematic Parameters for Assessment of Temporomandibular Joint Function and Disfunction: A Systematic Review and Meta-Analysis
Authors: Scolaro Alessandra , Khijmatgar Shahnawaz , Rai Pooja Mali , Falsarone Francesca , Alicchio Francesca , Mosca Arianna , Greco Christian , Del Fabbro Massimo , Tartaglia Gianluca Martino
Published: June 2022

The aim of this review was to answer the following PICO question: “Do TMJ kinematic parameters (intervention and comparison) show efficacy for assessment of mandibular function (Outcome) both in asymptomatic and TMD subjects? (Population)”. PubMed, Scopus, Web of Science, Embase, Central databases were searched. The inclusion criteria were (1) performed on human, (2) English only, (3) on healthy, symptomatic or surgically altered TMJ, (4) measured dynamic kinematics of mandible or TMJ (5) with six degrees of freedom. To assess the Risk of Bias, the Joanna Briggs Institute tool for non-randomised clinical studies was employed. A pairwise meta-analysis was carried out using STATA v.17.0 (Stata). The heterogeneity was estimated using the Q value and the inconsistency index. Ninety-two articles were included in qualitative synthesis, nine studies in quantitative synthesis. The condylar inclination was significantly increased in female (effect size 0.03°, 95% CI: −0.06, 0.12, p = 0.00). Maximum mouth opening (MMO) was increased significantly in female population in comparison with males (effect size 0.65 millimetres (0.36, 1.66). Incisor displacement at MMO showed higher values for control groups compared with TMD subjects (overall effect size 0.16 millimetres (−0.37, 0.69). Evidence is still needed, considering the great variety of devices and parameters used for arthrokinematics. The present study suggests standardising outcomes, design, and population of the future studies in order to obtain more reliable and repeatable values.
Universal Chromatic Resin-Based Composites: Aging Behavior Quantified by Quasi-Static and Viscoelastic Behavior Analysis
Authors: Ilie Nicoleta
Published: June 2022

Universal chromatic dental resin-based composites were recently developed in an attempt to speed up the restoration process with the aim of making it easier for the practitioner to decide on a suitable shade and to avoid time-consuming matching and mixing of materials. The way in which color is created in the analyzed universal chromatic materials is innovative, as it is not only induced by selective light absorption via pigments (Venus Diamond ONE, Venus Pearl ONE), as is usual in regular composites (Charisma Classic, Charisma Topaz, Venus, Venus Diamond), but also by selective light reflection via particularized microstructures (Omnichroma). Material properties were assessed at 24 h post-polymerization and after artificial aging. Flexural strength (n = 20) and modulus were measured in a 3-point-bending test and complemented with fractography and Weibull analysis. Quasi-static (Martens, Vickers, and indentation hardness; elastic and total indentation work; creep, indentation depth) and viscoelastic (storage, loss, and indentation moduli; loss factor) behavior (n = 6) was measured by a depth-sensing indentation test equipped with a DMA module. The nanoscale silica/zirconia polymer core-shell structure in the structural-colored material induces similar or poorer mechanical properties compared with pigment-colored materials, which is related to the higher polymer content. For all materials, aging shows a clear influence on the measured properties, with the degree of degradation depending on the measurement scale.
Image Analysis Semi-Automatic System for Colony-Forming-Unit Counting
Authors: Rodrigues Pedro Miguel , Luís Jorge , Tavaria Freni Kekhasharú
Published: June 2022

Background: Accurate quantitative analysis of microorganisms is recognized as an essential tool for gauging safety and quality in microbiology settings in a wide range of fields. The enumeration process of viable microorganisms via traditional culturing techniques are methodically convenient and cost-effective, conferring high applicability worldwide. However, manual counting can be time-consuming, laborious and imprecise. Furthermore, particular cases require an urgent and accurate response for effective processing. Methods: To reduce time limitations and discrepancies, this work introduces an image processing method capable of semi-automatically quantifying the number of colony forming units (CFUs). This rapid enumeration technique enables the technician to provide an expeditious assessment of the microbial load of a given sample. To test and validate the system, three bacterial species were cultured, and a labeled database was created, with subsequent image acquisition. Results: The system demonstrated acceptable classification measures; the mean values of Accuracy, Recall and F-measure were: (1) 95%, 95% and 0.95 for E. coli; (2) 91%, 91% and 0.90 for P. aeruginosa; and (3) 84%, 86% and 0.85 for S. aureus. Conclusions: Evidence related to the time-saving potential of the system was achieved; the time spent on quantification tasks of plates with a high number of colonies might be reduced to a half and occasionally to a third.
Influence of Different Load Conditions on Lower Extremity Biomechanics during the Lunge Squat in Novice Men
Authors: Gao Lidong , Lu Zhenghui , Liang Minjun , Baker Julien S. , Gu Yaodong
Published: June 2022

Objective: The lunge squat is one of the exercises to strengthen the lower limbs, however, there is little evidence of the effects of different equipment. The purpose of this study was to investigate the biomechanical effects of different types of equipment and loads on the lunge squat’s effect on the lower limbs. Methods: Fourteen male fitness novices participated in the experiment. Kinematics and kinetics in the sagittal plane using dumbbells, barbells, and weighted vests were measured using OpenSim. Two-way repeated measures ANOVA and one-dimensional statistical parametric mapping were used in the statistical analysis (SPM1D). Results: Range of motion (ROM) change in the knee joint was more obvious when using a barbell, whereas ROM when using a dumbbell was minimal. Compared to other joints, the joint moment at the hip joint was the largest and changed more significantly with increasing weight-bearing intensity, and the change was more pronounced with the dumbbell. For the center of pressure (COP) overall displacement, the dumbbell produced a smaller range of displacement. Conclusions: Dumbbells are suggested for male beginners to improve stability, barbells for the more experienced, and a low-weighted vest may be more appropriate for those with knee pain.
Potential Therapeutic Effect of All-Trans Retinoic Acid on Atherosclerosis
Authors: Deng Qile , Chen Jixiang
Published: June 2022

Atherosclerosis is a major risk factor for myocardial infarction and ischemic stroke, which are the leading cause of death worldwide. All-trans retinoic acid (ATRA) is a natural derivative of essential vitamin A. Numerous studies have shown that ATRA plays an important role in cell proliferation, cell apoptosis, cell differentiation, and embryonic development. All-trans retinoic acid (ATRA) is a ligand of retinoic acid receptors that regulates various biological processes by activating retinoic acid signals. In this paper, the metabolic processes of ATRA were reviewed, with emphasis on the effects of ATRA on inflammatory cells involved in the process of atherosclerosis.
Modulation of Vasomotor Function by Perivascular Adipose Tissue of Renal Artery Depends on Severity of Arterial Dysfunction to Nitric Oxide and Severity of Metabolic Parameters
Authors: Kagota Satomi , Futokoro Risa , McGuire John J. , Maruyama-Fumoto Kana , Shinozuka Kazumasa
Published: June 2022

Perivascular adipose tissue (PVAT) enhances vascular relaxation of mesenteric arteries in SHRSP.Z-Leprfa/IzmDmcr rats (SPZF), a metabolic syndrome model. We investigated and compared the effects of PVAT on the renal artery in SPZF with those on SHR/NDmcr-cp rats (CP). Renal arteries with and without PVAT were isolated from 23-week-old SPZF and CP. The effects of PVAT on acetylcholine- and nitroprusside-induced relaxation were examined using bioassays with phenylephrine-contracted arterial rings. Acetylcholine-induced relaxations without PVAT in SPZF and CP were 0.7- and 0.5-times lower in females than in males, respectively. In the presence of PVAT, acetylcholine-induced relaxations increased 1.4- and 2-times in male and female CP, respectively, but did not differ in SPZF. Nitroprusside-induced relaxation with and without PVAT was 0.7-times lower in female than in male SPZF but did not differ in CP. Angiotensin-II type-1 receptor (AT1R)/AT1R-associated protein mRNA ratios were lower in CP than in the SPZF and negatively correlated with the difference in arterial relaxation with and without PVAT. The effects of renal artery PVAT differed between the SPZF and CP groups. Higher levels of enhanced AT1R activity in SPZF PVAT may drive these differences by impairing the vascular smooth muscle responses to nitric oxide.
Chemical Barrier Proteins in Human Body Fluids
Authors: Kalló Gergő , Kumar Ajneesh , Tőzsér József , Csősz Éva
Published: June 2022

Chemical barriers are composed of those sites of the human body where potential pathogens can contact the host cells. A chemical barrier is made up by different proteins that are part of the antimicrobial and immunomodulatory protein/peptide (AMP) family. Proteins of the AMP family exert antibacterial, antiviral, and/or antifungal activity and can modulate the immune system. Besides these proteins, a wide range of proteases and protease inhibitors can also be found in the chemical barriers maintaining a proteolytic balance in the host and/or the pathogens. In this review, we aimed to identify the chemical barrier components in nine human body fluids. The interaction networks of the chemical barrier proteins in each examined body fluid were generated as well.
Intraductal Papillary Mucinous Neoplasms in Hereditary Cancer Syndromes
Authors: Ardeshna Devarshi R. , Rangwani Shiva , Cao Troy , Pawlik Timothy M. , Stanich Peter P. , Krishna Somashekar G.
Published: June 2022

Hereditary pancreatic cancer, which includes patients with familial pancreatic cancer (FPC) and hereditary pancreatic cancer syndromes, accounts for about 10% of all pancreatic cancer diagnoses. The early detection of pre-cancerous pancreatic cysts has increasingly become a focus of interest in recent years as a potential avenue to lower pancreatic cancer incidence and mortality. Intraductal papillary mucinous cystic neoplasms (IPMNs) are recognized precursor lesions of pancreatic cancer. IPMNs have high prevalence in patients with hereditary pancreatic cancer and their relatives. While various somatic mutations have been identified in IPMNs, certain germline mutations associated with hereditary cancer syndromes have also been identified in IPMNs, suggesting a role in their formation. While the significance for the higher prevalence of IPMNs or similar germline mutations in these high-risk patients remain unclear, IPMNs do represent pre-malignant lesions that need close surveillance. This review summarizes the available literature on the incidence and prevalence of IPMNs in inherited genetic predisposition syndromes and FPC and speculates if IPMN and pancreatic cancer surveillance in these high-risk individuals needs to change.
TIRAP/Mal Positively Regulates TLR8-Mediated Signaling via IRF5 in Human Cells
Authors: Nilsen Kaja Elisabeth , Skjesol Astrid , Frengen Kojen June , Espevik Terje , Stenvik Jørgen , Yurchenko Maria
Published: June 2022

Toll-like receptor 8 (TLR8) recognizes single-stranded RNA of viral and bacterial origin as well as mediates the secretion of pro-inflammatory cytokines and type I interferons by human monocytes and macrophages. TLR8, as other endosomal TLRs, utilizes the MyD88 adaptor protein for initiation of signaling from endosomes. Here, we addressed the potential role of the Toll-interleukin 1 receptor domain-containing adaptor protein (TIRAP) in the regulation of TLR8 signaling in human primary monocyte-derived macrophages (MDMs). To accomplish this, we performed TIRAP gene silencing, followed by the stimulation of cells with synthetic ligands or live bacteria. Cytokine-gene expression and secretion were analyzed by quantitative PCR or Bioplex assays, respectively, while nuclear translocation of transcription factors was addressed by immunofluorescence and imaging, as well as by cell fractionation and immunoblotting. Immunoprecipitation and Akt inhibitors were also used to dissect the signaling mechanisms. Overall, we show that TIRAP is recruited to the TLR8 Myddosome signaling complex, where TIRAP contributes to Akt-kinase activation and the nuclear translocation of interferon regulatory factor 5 (IRF5). Recruitment of TIRAP to the TLR8 signaling complex promotes the expression and secretion of the IRF5-dependent cytokines IFNβ and IL-12p70 as well as, to a lesser degree, TNF. These findings reveal a new and unconventional role of TIRAP in innate immune defense.
The Role of Mitochondrial Metabolism, AMPK-SIRT Mediated Pathway, LncRNA and MicroRNA in Osteoarthritis
Authors: Liu Hao-Yu , Chang Chi-Fen , Lu Cheng-Chang , Wu Shun-Cheng , Huang Bin , Cheng Tsung-Lin , Lin Sung-Yen , Ho Cheng-Jung , Lee Mon-Juan , Yang Chung-Da , Wang Ying-Chun , Li Jhong-You , Liu Ping-Cheng , Wei Chun-Wang , Kang Lin , Chen Chung-Hwan
Published: June 2022

Osteoarthritis (OA) is the most common joint disease characterized by degeneration of articular cartilage and causes severe joint pain, physical disability, and impaired quality of life. Recently, it was found that mitochondria not only act as a powerhouse of cells that provide energy for cellular metabolism, but are also involved in crucial pathways responsible for maintaining chondrocyte physiology. Therefore, a growing amount of evidence emphasizes that impairment of mitochondrial function is associated with OA pathogenesis; however, the exact mechanism is not well known. Moreover, the AMP-activated protein kinase (AMPK)–Sirtuin (SIRT) signaling pathway, long non-coding RNA (lncRNA), and microRNA (miRNA) are important for regulating the physiological and pathological processes of chondrocytes, indicating that these may be targets for OA treatment. In this review, we first focus on the importance of mitochondria metabolic dysregulation related to OA. Then, we show recent evidence on the AMPK-SIRT mediated pathway associated with OA pathogenesis and potential treatment options. Finally, we discuss current research into the effects of lncRNA and miRNA on OA progression or inhibition.
Analysis of Melanoma Gene Expression Signatures at the Single-Cell Level Uncovers 45-Gene Signature Related to Prognosis
Authors: Bakr Mohamed Nabil , Takahashi Haruko , Kikuchi Yutaka
Published: June 2022

Since the current melanoma clinicopathological staging system remains restricted to predicting survival outcomes, establishing precise prognostic targets is needed. Here, we used gene expression signature (GES) classification and Cox regression analyses to biologically characterize melanoma cells at the single-cell level and construct a prognosis-related gene signature for melanoma. By analyzing publicly available scRNA-seq data, we identified six distinct GESs (named: “Anti-apoptosis”, “Immune cell interactions”, “Melanogenesis”, “Ribosomal biogenesis”, “Extracellular structure organization”, and “Epithelial-Mesenchymal Transition (EMT)”). We verified these GESs in the bulk RNA-seq data of patients with skin cutaneous melanoma (SKCM) from The Cancer Genome Atlas (TCGA). Four GESs (“Immune cell interactions”, “Melanogenesis”, “Ribosomal biogenesis”, and “Extracellular structure organization”) were significantly correlated with prognosis (p = 1.08 × 10−5, p = 0.042, p = 0.001, and p = 0.031, respectively). We identified a prognostic signature of melanoma composed of 45 genes (MPS_45). MPS_45 was validated in TCGA-SKCM (HR = 1.82, p = 9.08 × 10−6) and three other melanoma datasets (GSE65904: HR = 1.73, p = 0.006; GSE19234: HR = 3.83, p = 0.002; and GSE53118: HR = 1.85, p = 0.037). MPS_45 was independently associated with survival (p = 0.002) and was proved to have a high potential for predicting prognosis in melanoma patients.
Gender Differences in Non-Persistence with Angiotensin-Converting Enzyme Inhibitors and Angiotensin Receptor Blockers among Older Hypertensive Patients with Peripheral Arterial Disease
Authors: Wawruch Martin , Murin Jan , Tesar Tomas , Paduchova Martina , Petrova Miriam , Celovska Denisa , Havelkova Beata , Trnka Michal , Masarykova Lucia , Alfian Sofa D. , Aarnio Emma
Published: June 2022

The beneficial effects of angiotensin-converting enzyme inhibitors (ACEIs) and angiotensin receptor blockers (ARBs) in hypertensive patients with peripheral arterial disease (PAD) depends on long-term persistence. The aims of our study were to analyse gender differences in non-persistence with ACEIs/ARBs, and to identify the characteristics associated with the likelihood of non-persistence. Our study cohort included 7080 hypertensive patients (4005 women and 3075 men) aged ≥65 years, treated with ACEIs/ARBs, in whom PAD was diagnosed between 1 January and 31 December 2012. Non-persistence was identified according to a treatment gap of 6 months without ACEI/ARB prescriptions. The characteristics associated with non-persistence were identified using the Cox regression model. At the end of the 5-year follow-up, 23.2% of the whole study cohort, 22.3% of men, and 23.9% of women were non-persistent with ACEIs/ARBs, with no significant gender differences in persistence. While a number of characteristics were associated with non-persistence, only three characteristics had consistent, statistically significant associations in both genders: being a new ACEI/ARB user increased the likelihood of non-persistence, and general practitioner as index prescriber and increasing the overall number of medications decreased the likelihood of non-persistence. Information on the differences in characteristics that are associated with non-persistence between genders may help to better identify patients for whom special attention should be paid to improve their persistence.
UFR2709, an Antagonist of Nicotinic Acetylcholine Receptors, Delays the Acquisition and Reduces Long-Term Ethanol Intake in Alcohol-Preferring UChB Bibulous Rats
Authors: Gálvez Gabriel , González-Gutiérrez Juan Pablo , Hödar-Salazar Martín , Sotomayor-Zárate Ramón , Quintanilla María Elena , Quilaqueo María Elena , Rivera-Meza Mario , Iturriaga-Vásquez Patricio
Published: June 2022

Alcoholism is a worldwide public health problem with high economic cost and which affects health and social behavior. It is estimated that alcoholism kills 3 million people globally, while in Chile it is responsible for around 9 thousand deaths per year. Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels expressed in the central nervous system, and they were suggested to modulate the ethanol mechanism involved in abuse and dependence. Previous work demonstrated a short-term treatment with UFR2709, a nAChRs antagonist, which reduced ethanol intake using a two-bottle free-choice paradigm in University of Chile bibulous (UChB) rats. Here, we present evidence of the UFR2709 efficacy in reducing the acquisition and long-term ethanol consumption. Our results show that UFR2709 (2.5 mg/kg i.p.) reduces the seek behavior and ethanol intake, even when the drug administration was stopped, and induced a reduction in the overall ethanol intake by around 55%. Using naïve UChB bibulous rats, we demonstrate that UFR2709 could delay and reduce the genetically adaptive impulse to seek and drink ethanol and prevent its excessive intake.
What We Have Learned from Animal Models to Understand the Etiology and Pathology of Endometrioma-Related Infertility
Authors: Tan Zhouyurong , Hung Sze-Wan , Zheng Xu , Wang Chi-Chiu , Chung Jacqueline Pui-Wah , Zhang Tao
Published: June 2022

Endometrioma (OMA) is the most common subtype of endometriosis, in which the endometriotic lesions are implanted in the ovary. Women with OMA are usually associated with infertility, presenting with reduced ovarian reserve, low oocyte quantity and quality, and poor fertility outcomes. However, the underlying pathological mechanisms in OMA-related infertility are still unclear. Due to the limitations and ethical issues of human studies in reproduction, animal models that recapitulate OMA characteristics and its related infertility are critical for mechanistic studies and subsequent drug development, preclinical testing, and clinical trials. This review summarized the investigations of OMA-related infertility based on previous and latest endometrioma models, providing the possible pathogenesis and potential therapeutic targets for further studies.
A Novel Framework for Optimizing Indoor Illuminance and Discovering Association of Involved Variables
Authors: Matin Negar Heidari , Eydgahi Ali , Gharipour Amin , Matin Payam
Published: June 2022

The associations between various design variables affecting the visual performance of responsive facade systems are investigated in this study. First, we propose a data-driven approach to study practical aspects of illuminance optimization for responsive facades. In this approach, the hourly indoor illuminance data are combined with the location information to generate an objective function. This function is then utilized to evaluate the visual performance of responsive facade systems by matching a variety of facade angle movements to hourly sunshine patterns. Next, statistical tests were deployed to evaluate the role of design variables in different scenarios. The results provide detailed information about the design variables and their effects on visual comfort at 0.05 significant levels. On average, facade angles, facade configurations, facade orientations, and facade locations were significant in 100%, 41%, 87%, and 45% of different possible combinations of scenarios/variables, respectively.
Study on Flexural Behavior of Self-Compacting Concrete Beams with Recycled Aggregates
Authors: Yu Fang , Wang Min , Yao Dali , Yang Weichuang
Published: June 2022

Currently, numerous studies have focused on the differences in the properties of self-compacting concrete with recycled aggregate (RASCC) and normal concrete (NC), while less attention has been paid to the application of RASCC in reinforced concrete structures. In this paper, four-point bending loading tests were performed on seven RASCC beams and four NC beams, considering the parameter of reinforcement ratio, and the flexural properties were analyzed and compared. The results showed that the failure form, moment–deflection curves, and flexural capacity of the RASCC beams were similar to those of the NC beams. However, the cracking moment and the crack width of the RASCC beams were significantly smaller than that of the NC beams. With an increase in the longitudinal reinforcement ratio, the cracking resistance and flexural capacity of the RASCC beams increased significantly. The cracking moment and flexural capacity could be calculated using the method of the Chinese code GB50010-2010. However, compared with the test values, the predicted deflection was slightly less safe, while the maximum crack width calculation was slightly more conservative. Therefore, the current code formula was revised according to the test results.
Correction: Zhao et al. Integration of Transcriptome, Proteome, and Metabolome Provides Insights into How Calcium Enhances the Mechanical Strength of Herbaceous Peony Inflorescence Stems. Cells 2019, 8, 102
Authors: Zhao Daqiu , Tang Yuhan , Xia Xing , Sun Jing , Meng Jiasong , Shang Jiali , Tao Jun
Published: June 2022

The authors wish to make the following changes to their paper [...]