bluuebunny/crossref_metadata_2025 huggingface dataset:
Structure and Phenology of Herbaceous Stratum in the Sahelian Rangelands of Senegal
Authors: Diatta Ousmane , Ngom Daouda , Ndiaye Ousmane , Diatta Sékouna , Taugourdeau Simon
Published: May 2023

This study describes the structure and phenology of Sahelian rangelands during the rainy season. It was undertaken on a grazed plot and a fenced plot. Measurements were taken every 10 days over two rainy seasons between July and October 2018 and between August and October 2019. The measurements included the phenological stage, the vegetative and flowering height, the coverage, and the phytomass. The results showed that phytomass did not systematically differ between grazed and fenced plots. Flowering started as early as mid-July, but most of the plants flowered in early October. Flowering and fruiting occurred earlier (based on the number of days after the first rain) in the late rainy season (2019) than in the early one (2018). These stages reached their peak in October; senescence began in October. Vegetative height, reproductive height, and coverage were similar between the two years. Height peaks were similar, but they were reached earlier (based on the number of days after the first rain) in the late rainy season than in the early one. Coverage peaks were similar (59.8% in the early rainy season vs. 65.8% in the late one). Vegetative height (around 30 cm), reproductive height (around 36 cm), and coverage (around 60%) reached their maximum in October, but reproductive height was greater than vegetative height. These parameters were lower at the grazed site compared to the fenced site, and flowering started earlier at the grazed site. The pasture had a negative impact on coverage, height, and early flowering. Structure and phenology were more sensitive than to changes in rainfall between years. Phenology results will help determine the best time to harvest the phytomass (phytomass stock), as the nutritional quality of the forage is known to decrease after fruiting.
Assessment of the Orthodontic External Resorption in Periodontal Breakdown—A Finite Elements Analysis (Part I)
Authors: Moga Radu Andrei , Olteanu Cristian Doru , Botez Mircea Daniel , Buru Stefan Marius
Published: May 2023

This Finite Elements Analysis (FEA) assessed the accuracy of Tresca failure criteria (maximum shear stress) for the study of external root resorption. Additionally, the tooth absorption–dissipation ability was assessed. Overall, 81 models of the second mandibular premolar, out of a total of 324 simulations, were involved. Five orthodontic movements (intrusion, extrusion, rotation, translation, and tipping) were simulated under 0.6 N and 1.2 N in a horizontal progressive periodontal breakdown simulation of 0–8 mm. In all simulations, Tresca criteria accurately displayed the localized areas of maximum stress prone to external resorption risks, seeming to be adequate for the study of the resorptive process. The localized areas were better displayed in the radicular dentine–cementum component than in the entire tooth structure. The rotation and translation seem prone to a higher risk of external root resorption after 4 mm of loss. The resorptive risks seem to increase along with the progression of periodontal breakdown if the same amount of applied force is guarded. The localized resorption-prone areas follow the progression of bone loss. The two light forces displayed similar extensions of maximum stress areas. The stress displayed in the coronal dentine decreases along with the progression of bone loss. The absorption–dissipation ability of the tooth is about 87.99–97.99% of the stress.
Analysing the Effects of Different Types of Exercise on Dyspnoea and Fatigue in Adults through COPD-Systematic Review and Meta-Analysis of Randomised Clinical Trials
Authors: Couto Nuno , Cid Luís , Alves Susana , Brito João Paulo , Pimenta Nuno , Bento Teresa
Published: May 2023

Background: Chronic obstructive pulmonary disease (COPD) is a heterogeneous lung condition, the main symptoms of which are dyspnoea and fatigue. Though exercise has been recommended for subjects with COPD, its benefits remain unclear. The aim of this study was to summarise, through a systematic review and meta-analysis, the available evidence on the effects of aerobic, resistance, stretching, and combined exercise on the main symptoms of COPD. Methods: Search was performed using the electronic databases PubMed and Web of Science. Randomised controlled trials (RCTs) with interventions based on aerobic, resistance and/or combined exercise published until July 2022 were identified. The effects were summarised based on standardised mean differences (95% confidence intervals) using random and fixed effect models. Results: Eight studies were selected, including a total of 375 subjects. The results obtained showed that resistance exercise, aerobic exercise and combined exercise seem to improve dyspnoea and fatigue symptoms in COPD subjects. Conclusions: In general, we can conclude that exercise-based interventions appear to improve the main COPD symptoms and may benefit quality of life in this population.
Prevalence of Possible Sleep Bruxism and Its Association with Social and Orofacial Factors in Preschool Population
Authors: Diéguez-Pérez Montserrat , Ticona-Flores Jesús Miguel , Prieto-Regueiro Beatriz
Published: May 2023

The prevalence of bruxism in the preschool population varies according to different investigations. The aim of this study was to investigate the prevalence of sleep bruxism and its relationship with social and orofacial factors in children aged 3 to 5 years. Three hundred forty-three preschool children were divided into two groups, one with nocturnal bruxism, as reported by parents, and another without this disorder. Questionnaires were distributed to the parents to determine the children’s family social status and parafunctional habits at the time of the study. The clinical inspection confirmed the presence of certain parafunctional habits and the children’s occlusal characteristics. The data obtained were analysed using descriptive statistics such as frequency chi-square tests to identify the influence of qualitative variables. Of the total sample, 28.9% of preschoolers presented sleep bruxism. The highest prevalence was observed in boys (61.6%) at the age of 5 years (41.4%). Characteristics associated with this pathology were lip incompetence, open bite, crossbite, and overbite, with p < 0.05. Sleep bruxism in preschool children has a higher prevalence in boys and is more frequently expressed from 5 years of age. Open bite, overbite, and crossbite should be considered factors associated with parafunction.
Characterization, Evolutionary Analysis, and Expression Pattern Analysis of the Heat Shock Transcription Factors and Drought Stress Response in Heimia myrtifolia
Authors: Zhang Guozhe , Gu Cuihua , Ye Yacheng , Zhao Yu , Shang Linxue , Shao Weili , Hong Sidan , Ma Jin
Published: May 2023

Heat shock transcription factors (HSFs) are among the most important regulators of plant responses to abiotic stimuli. They play a key role in numerous transcriptional regulatory processes. However, the specific characteristics of HSF gene family members and their expression patterns in different tissues and under drought stress have not been precisely investigated in Heimia myrtifolia. This study analyzed transcriptome data from H. myrtifolia and identified 15 members of the HSF family. Using a phylogenetic tree, these members were classified into three major classes and fifteen groups. The amino acid physicochemical properties of these members were also investigated. The results showed that all HmHSF genes are located in the nucleus, and multiple sequence alignment analysis revealed that all HmHSF proteins have the most conserved DBD structural domains. Interestingly, a special HmHSF15 protein was found in the three-dimensional structure of the protein, which has a conserved structural domain that performs a function in addition to the unique structural domain of HSF proteins, resulting in a three-dimensional structure for HmHSF15 that is different from other HmHSF proteins. GO enrichment analysis shows that most HmHSFA-like genes are part of various biological processes associated with abiotic stresses. Finally, this study analyzed the tissue specificity of HmHSF genes in different parts of H. myrtifolia by qRT-PCR and found that HmHSF genes were more abundantly expressed in roots than in other tissues, and HmHSF05, HmHSF12, and HmHSF14 genes were different from other HSF genes, which could be further analyzed to verify their functionality. The results provide a basis for analyzing the functions of HmHSF genes in H. myrtifolia and help to explore the molecular regulatory mechanism of HmHSF in response to drought stress.
Natural Gas Pyrolysis in a Liquid Metal Bubble Column Reaction System—Part I: Experimental Setup and Methods
Authors: Hofberger Christoph Michael , Dietrich Benjamin , Durán Vera Inés , Krumholz Ralf , Stoppel Leonid , Uhlenbruck Neele , Wetzel Thomas
Published: May 2023

Hydrogen is not only an important industrial chemical but also an energy carrier with increasing demand. However, the current production techniques are based on technologies that result in massive CO2 emissions. In contrast, the pyrolysis of alkanes in a liquid metal bubble column reactor does not lead to direct CO2 emissions. In order to transfer this technology from lab-scale to industrial applications, it has to be scaled up and the influences of the most common constituent of natural gas on the pyrolysis process have to be determined. For this study, the liquid metal bubble column technology developed at the KIT was scaled up by a factor of 3.75, referred to as the reactor volume. In this article, the experimental setup containing the reactor is described in detail. In addition, new methods for the evaluation of experimental data will be presented. The reactor, as well as the experimental results from pure methane pyrolysis (PM), will be compared to the previous generation of reactors in terms of methane conversion. It could be proven that scaling up the reactor volume did not result in a decrease in methane conversion. For part II of this publication, methane-ethane (MEM) gas mixtures and high calorific natural gas (nGH) were pyrolyzed, and the results were discussed on the basis of the present part I.
Molecular Characterization of Prunus lusitanica L. Fruit Extracts and Their Health-Promoting Potential in Inflammation, Diabetes, and Neurodegenerative Diseases
Authors: Abraão Ana , Martins-Gomes Carlos , Domínguez-Perles Raúl , Barros Ana , Silva Amélia M.
Published: May 2023

Prunus lusitanica L. is a shrub belonging to the genus Prunus L. (Rosaceae family) that produces small fruits with none known application. Thus, the aim of this study was to determine the phenolic profile and some health-promoting activities of hydroethanolic (HE) extracts obtained from P. lusitanica fruits, harvested from three different locations. Qualitative and quantitative analysis of extracts was performed using HPLC/DAD-ESI-MS and antioxidant activity was assessed by in vitro methods. Antiproliferative/cytotoxic activity was determined on Caco-2, HepG2, and RAW 264.7 cells, anti-inflammatory activity was assessed using lipopolysaccharide (LPS)-stimulated RAW 264.7 cells, and the antidiabetic, antiaging, and neurobiological action of extracts was determined in vitro by assessing their inhibitory effect against the activity of α-amylase, α-glucosidase, elastase, tyrosinase, and acetylcholinesterase (AChE). Results showed that P. lusitanica fruit HE extracts from the three different locations showed identical phytochemical profile and bioactivities, although small differences were observed regarding the quantities of some compounds. Extracts of P. lusitanica fruits contain high levels in total phenolic compounds, namely, hydroxycinnamic acids, as well as flavan-3-ols and anthocyanins, primarily cyanidin-3-(6-trans-p-coumaroyl)glucoside. P. lusitanica fruit extracts have a low cytotoxic/antiproliferative effect, with the lowest IC50 value obtained in HepG2 cells (352.6 ± 10.0 μg/mL, at 48 h exposure), but high anti-inflammatory activity (50–60% NO release inhibition, at 100 μg/mL extract) and neuroprotective potential (35–39% AChE inhibition, at 1 mg/mL), and moderate antiaging (9–15% tyrosinase inhibition, at 1 mg/mL) and antidiabetic (9–15% α-glucosidase inhibition, at 1 mg/mL) effects. The bioactive molecules present in the fruits of P. lusitanica deserve to be further explored for the development of new drugs of interest to the pharmaceutical and cosmetic industry.
Lavandula Species, Their Bioactive Phytochemicals, and Their Biosynthetic Regulation
Authors: Habán Miroslav , Korczyk-Szabó Joanna , Čerteková Simona , Ražná Katarína
Published: May 2023

Lavandula species are one of the most useful aromatic and medicinal plants and have great economic potential. The phytopharmaceutical contribution of the secondary metabolites of the species is unquestionable. Most recent studies have been focusing on the elucidation of the genetic background of secondary metabolite production in lavender species. Therefore, knowledge of not only genetic but especially epigenetic mechanisms for the regulation of secondary metabolites is necessary for the modification of those biosynthesis processes and the understanding of genotypic differences in the content and compositional variability of these products. The review discusses the genetic diversity of Lavandula species in relation to the geographic area, occurrence, and morphogenetic factors. The role of microRNAs in secondary-metabolites biosynthesis is described.
Enhanced Energetic Performance via the Combination of Furoxan and Oxa-[5,5]bicyclic Structures
Authors: Zhang Qi , Zhang Xun , Pang Siping , He Chunlin
Published: May 2023

Three new compounds based on the combination of furoxan (1,2,5-oxadiazole N-oxide) and oxa-[5,5]bicyclic ring were synthesized. Among them, the nitro compound showed satisfactory detonation properties (Dv, 8565 m s−1; P, 31.9 GPa), which is comparable to the performance of RDX (a classic high-energy secondary explosive). Additionally, the introduction of the N-oxide moiety and oxidation of the amino group more effectively improved the oxygen balance and density (d, 1.81 g cm−3; OB%, +2.8%) of the compounds compared to furazan analogues. Combined with good density and oxygen balance as well as moderate sensitivity, this type of furoxan and oxa-[5,5]bicyclic structure will open up a platform for the synthesis and design of new high-energy materials.
Physapruin A Exerts Endoplasmic Reticulum Stress to Trigger Breast Cancer Cell Apoptosis via Oxidative Stress
Authors: Yu Tzu-Jung , Shiau Jun-Ping , Tang Jen-Yang , Farooqi Ammad Ahmad , Cheng Yuan-Bin , Hou Ming-Feng , Yen Chia-Hung , Chang Hsueh-Wei
Published: May 2023

Physalis plants are commonly used traditional medicinal herbs, and most of their extracts containing withanolides show anticancer effects. Physapruin A (PHA), a withanolide isolated from P. peruviana, shows antiproliferative effects on breast cancer cells involving oxidative stress, apoptosis, and autophagy. However, the other oxidative stress-associated response, such as endoplasmic reticulum (ER) stress, and its participation in regulating apoptosis in PHA-treated breast cancer cells remain unclear. This study aims to explore the function of oxidative stress and ER stress in modulating the proliferation and apoptosis of breast cancer cells treated with PHA. PHA induced a more significant ER expansion and aggresome formation of breast cancer cells (MCF7 and MDA-MB-231). The mRNA and protein levels of ER stress-responsive genes (IRE1α and BIP) were upregulated by PHA in breast cancer cells. The co-treatment of PHA with the ER stress-inducer (thapsigargin, TG), i.e., TG/PHA, demonstrated synergistic antiproliferation, reactive oxygen species generation, subG1 accumulation, and apoptosis (annexin V and caspases 3/8 activation) as examined by ATP assay, flow cytometry, and western blotting. These ER stress responses, their associated antiproliferation, and apoptosis changes were partly alleviated by the N-acetylcysteine, an oxidative stress inhibitor. Taken together, PHA exhibits ER stress-inducing function to promote antiproliferation and apoptosis of breast cancer cells involving oxidative stress.
Myristica fragrans Extract Inhibits Platelet Desialylation and Activation to Ameliorate Sepsis-Associated Thrombocytopenia in a Murine CLP-Induced Sepsis Model
Authors: Jeong Seong-Hun , Park Ji-Young , Ryu Young Bae , Kim Woo Sik , Lee In-Chul , Kim Ju-Hong , Kim Dohoon , Ha Ji-Hye , Lee Ba-Wool , Nam Jiyoung , Cho Kyoung-Oh , Kwon Hyung-Jun
Published: May 2023

Sepsis, characterized by an uncontrolled host inflammatory response to infections, remains a leading cause of death in critically ill patients worldwide. Sepsis-associated thrombocytopenia (SAT), a common disease in patients with sepsis, is an indicator of disease severity. Therefore, alleviating SAT is an important aspect of sepsis treatment; however, platelet transfusion is the only available treatment strategy for SAT. The pathogenesis of SAT involves increased platelet desialylation and activation. In this study, we investigated the effects of Myristica fragrans ethanol extract (MF) on sepsis and SAT. Desialylation and activation of platelets treated with sialidase and adenosine diphosphate (platelet agonist) were assessed using flow cytometry. The extract inhibited platelet desialylation and activation via inhibiting bacterial sialidase activity in washed platelets. Moreover, MF improved survival and reduced organ damage and inflammation in a mouse model of cecal ligation and puncture (CLP)-induced sepsis. It also prevented platelet desialylation and activation via inhibiting circulating sialidase activity, while maintaining platelet count. Inhibition of platelet desialylation reduces hepatic Ashwell–Morell receptor-mediated platelet clearance, thereby reducing hepatic JAK2/STAT3 phosphorylation and thrombopoietin mRNA expression. This study lays a foundation for the development of plant-derived therapeutics for sepsis and SAT and provides insights into sialidase-inhibition-based sepsis treatment strategies.
Caveolin-1 and Atherosclerosis: Regulation of LDLs Fate in Endothelial Cells
Authors: Puddu Alessandra , Montecucco Fabrizio , Maggi Davide
Published: May 2023

Caveolae are 50–100 nm cell surface plasma membrane invaginations observed in terminally differentiated cells. They are characterized by the presence of the protein marker caveolin-1. Caveolae and caveolin-1 are involved in regulating several signal transduction pathways and processes. It is well recognized that they have a central role as regulators of atherosclerosis. Caveolin-1 and caveolae are present in most of the cells involved in the development of atherosclerosis, including endothelial cells, macrophages, and smooth muscle cells, with evidence of either pro- or anti-atherogenic functions depending on the cell type examined. Here, we focused on the role of caveolin-1 in the regulation of the LDLs’ fate in endothelial cells.
Monitoring Treatment Response, Early Recurrence, and Survival in Uterine Serous Carcinoma and Carcinosarcoma Patients Using Personalized Circulating Tumor DNA Biomarkers
Authors: Bellone Stefania , McNamara Blair , Mutlu Levent , Demirkiran Cem , Hartwich Tobias Max Philipp , Harold Justin , Yang-Hartwich Yang , Siegel Eric R. , Santin Alessandro D.
Published: May 2023

Uterine serous carcinoma (USC) and carcinosarcomas (CSs) are rare, highly aggressive variants of endometrial cancer. No reliable tumor biomarkers are currently available to guide response to treatment or detection of early recurrence in USC/CS patients. Circulating tumor DNA (ctDNA) identified using ultrasensitive technology such as droplet digital polymerase chain reaction (ddPCR) may represent a novel platform for the identification of occult disease. We explored the use of personalized ctDNA markers for monitoring USC and CS patients. Tumor and plasma samples from USC/CS patients were collected at the time of surgery and/or during the treatment course for assessment of tumor-specific somatic structural variants (SSVs) by a clinical-grade next-generation sequencing (NGS) platform (i.e., Foundation Medicine) and a droplet digital PCR instrument (Raindance, ddPCR). The level of ctDNA was quantified by droplet digital PCR in plasma samples and correlated to clinical findings, including CA-125 serum and/or computed tomography (CT) scanning results. The genomic-profiling-based assay identified mutated “driver” target genes for ctDNA analysis in all USC/CS patients. In multiple patients, longitudinal ctDNA testing was able to detect the presence of cancer cells before the recurrent tumor was clinically detectable by either CA-125 or CT scanning. Persistent undetectable levels of ctDNA following initial treatment were associated with prolonged progression-free and overall survival. In a USC patient, CA-125 and TP53 mutations but not PIK3CA mutations become undetectable in the plasma at the time of recurrence, suggesting that more than one customized probe should be used for monitoring ctDNA. Longitudinal ctDNA testing using tumor-informed assays may identify the presence of residual tumors, predict responses to treatment, and identify early recurrences in USC/CS patients. Recognition of disease persistence and/or recurrence through ctDNA surveillance may allow earlier treatment of recurrent disease and has the potential to change clinical practice in the management of USC and CS patients. CtDNA validation studies in USC/CS patients prospectively enrolled in treatment trials are warranted.
Curcumin: Overview of Extraction Methods, Health Benefits, and Encapsulation and Delivery Using Microemulsions and Nanoemulsions
Authors: Ciuca Maria D. , Racovita Radu C.
Published: May 2023

Curcumin is the principal curcuminoid found in the rhizomes of turmeric. Due to its therapeutic action against cancer, depression, diabetes, some bacteria, and oxidative stress, it has been used widely in medicine since ancient times. Due to its low solubility, the human organism cannot completely absorb it. Advanced extraction technologies, followed by encapsulation in microemulsion and nanoemulsion systems, are currently being used to improve bioavailability. This review discusses the different methods available for curcumin extraction from plant material, methods for the identification of curcumin in the resulting extracts, its beneficial effects on human health, and the encapsulation techniques into small colloidal systems that have been used over the past decade to deliver this compound.
AR/VR Teaching-Learning Experiences in Higher Education Institutions (HEI): A Systematic Literature Review
Authors: Bermejo Belen , Juiz Carlos , Cortes David , Oskam Jeroen , Moilanen Teemu , Loijas Jouko , Govender Praneschen , Hussey Jennifer , Schmidt Alexander Lennart , Burbach Ralf , King Daniel , O'Connor Colin , Dunlea Davin
Published: May 2023

During the last few years, learning techniques have changed, both in basic education and in higher education. This change has been accompanied by new technologies such as Augmented Reality (AR) and Virtual Reality (AR). The combination of these technologies in education has allowed a greater immersion, positively affecting the learning and teaching processes. In addition, since the COVID-19 pandemic, this trend has been growing due to the diversity of the different fields of application of these technologies, such as heterogeneity in their combination and their different experiences. It is necessary to review the state of the art to determine the effectiveness of the application of these technologies in the field of university higher education. In the present paper, this aim is achieved by performing a systematic literature review from 2012 to 2022. A total of 129 papers were analyzed. Studies in our review concluded that the application of AR/VR improves learning immersion, especially in hospitality, medicine, and science studies. However, there are also negative effects of using these technologies, such as visual exhaustion and mental fatigue.
Periodic Density Functional Theory (PDFT) Simulating Crystal Structures with Microporous CHA Framework: An Accuracy and Efficiency Study
Authors: Chen Xiao-Fang
Published: May 2023

The structure property is the fundamental factor in determining the stability, adsorption, catalytic performance, and selectivity of microporous materials. Seven density functional approximations (DFAs) are used to simulate the crystal structure of microporous material for examining the efficiency and accuracy. In comparison with the existing zeolites, microporous materials with CHA framework are selected as the testing model. The calculation results indicate that the least lattice volume deviation is 5.18/2.72 Å3 from PBE_mGGA, and the second least is −5.55/−10.36 Å3 from LDA_PP. Contrary to USPP_LDA, PBE_GW, PAW_PBE, and PAW_GGA overestimate the lattice volume by ~15.00–20.00 Å3. For each method, RMS deviations are less than 0.016 Å for bond length and less than 2.813° for bond angle. To complete the crystal structure calculation, the CPU time reduces in order of USPP_GGA > PBE_GW > PAW_GGA, PBE_mGGA > PAW_PBE > LDA_PP > USPP_LDA. For two testing models, when the calculation time is not important, PBE_mGGA is the best choice, and when the tradeoff between accuracy and efficiency is considered, LDA_PP is preferred. It seems feasible and efficient to simulate the zeolite structure through E-V curve fitting, full optimization, and phonon analysis bythe periodic density functional theory.
Different Subtypes of Osteosarcoma: Histopathological Patterns and Clinical Behaviour
Authors: Rothzerg Emel , Xu Jiake , Wood David
Published: May 2023

Osteosarcoma (OS) is a primary malignant bone tumour that usually occurs in children and adolescents. OS is a highly aggressive tumour type with a propensity for local invasion and systemic early metastasis to the lungs or other bones. According to the World Health Organization, there are different subtypes of OS, including conventional OS (osteoblastic, chondroblastic, fibroblastic), telangiectatic OS, low-grade OS, small-cell OS, parosteal OS, periosteal OS, and high-grade surface OS. In this mini review, we will discuss the background of OS and histopathological patterns and clinical behaviour of the disease. Understanding the subtypes of OS and their pathogenesis is crucial for developing more precise and effective therapies for OS patients.
Abundance and Distribution of Macro- and Mesoplastic Debris on Selected Beaches in the Northern Strait of Malacca
Authors: Lim Er Vin , Nilamani Nithiyaa , Razalli Norhanis M. , Zhang Shoufeng , Li Hongjun , Haron Muhammad Lutfi , Abdullah Anisah Lee , Yasin Zulfigar , Zanuri Norlaila Mohd , Tan Shau Hwai Aileen
Published: May 2023

Plastics account for 60–80% of marine debris worldwide, and, in 2021, Malaysia was the 28th largest plastic polluter in the world. In light of this finding, the Malaysian government has launched the Roadmap Towards Zero Single-Use Plastics 2018–2030 and the Plastics Sustainability Roadmap 2021–2030 to reduce plastic pollution and implement a circular economy for Malaysia. A comprehensive database of the status of plastic pollution in Malaysia is needed to achieve this target. This study aims to record the presence of macro- (>2.5 cm) and mesoplastic (0.5–2.5 cm) debris at selected beaches in the northern Strait of Malacca. All study sites are publicly accessible beaches (Pulau Songsong, Teluk Aling, and Pulau Gazumbo) except Pulau Lembu, which is in a Marine Protected Area (MPA). The debris was collected from predetermined transects on the beach and categorised according to its form and economic market segments in Malaysia. Most of the macro- (53–75% of total mass) and mesoplastics (52–80% of the total number) were accumulated in the backshore area. Public beaches such as Pulau Gazumbo and Pulau Songsong recorded the highest abundance of macroplastics, with 7.32 g/m2 and 9.77 g/m2, respectively. Teluk Aling recorded the lowest abundance of macroplastics (3.58 g/m2) but the highest in mesoplastics (0.55 items/m2). Most of the macroplastics found were packaging plastics such as plastic bottles, containers, and polystyrene foam debris. Although Pulau Lembu is an MPA, the amount of macroplastics found was considerably high (7.17 g/m2). Based on the beach cleanliness index, Pulau Gazumbo (−3.99) was the dirtiest site, followed by Pulau Lembu (−2.92) and Pulau Songsong (−2.85), while Teluk Aling (−1.63) was the cleanest site, which can explain the amount of macroplastic debris found. However, all the study sites’ cleanliness may not be ideal, as the indexes were less than zero due to the low availability of waste bins and insufficient frequency of beach cleaning. This may not be able to curb the effects of high anthropogenic activities conducted in addition to uncontrollable natural factors.
Safety Assessment of Coastal Bridge Superstructures with Box Girders under Potential Landslide Tsunamis
Authors: Han Wanshui , Xu Xin , Wang Jiajia , Xiao Lili , Zhou Kai , Guo Xuelian
Published: May 2023

The superstructure of a coastal bridge is prone to overturn or unseating under a catastrophic tsunami, which seriously affects the post-disaster emergency rescue. In this paper, we establish a safety assessment framework for the superstructure of a bridge with a box girder under a potential landslide tsunami, and apply it to an in-service box girder and Baiyun Slide Complex on the southeast coast of China. First, a meshless numerical approach called Tsunami Squares (TS) is used to predict the movement of landslides and tsunamis. Additionally, we introduce the velocity-weakening basal friction effect in the model to optimize the landslide dynamics. Second, the maximum lateral and vertical wave loads on a box girder can be estimated using the time series of the wave height and velocity in the TS model. Third, we construct a safety evaluation method for the superstructure using the reaction of the bearing as the critical index. The results indicate that the framework developed here provides instructive guidance for evaluating the safety of coastal bridge superstructures during tsunami disasters, and we discuss the influence of the basal friction effect, bridge elevation, and support type on the structural safety.
Essential Role of CgErg6p in Maintaining Oxidative Stress Tolerance and Iron Homeostasis in Candida glabrata
Authors: Elias Daniel , Tóth Hervay Nora , Bujdos Marek , Gbelska Yvetta
Published: May 2023

The human pathogenic fungus Candida glabrata is the second leading cause of candidemia, a life-threatening invasive mycosis. Clinical outcomes are complicated by reduced susceptibility of C. glabrata to azoles together with its ability to evolve stable resistance to both azoles and echinocandins following drug exposure. Compared to other Candida spp., C. glabrata displays robust oxidative stress resistance. In this study, we investigated the impact of CgERG6 gene deletion on the oxidative stress response in C. glabrata. CgERG6 gene encodes sterol-24-C-methyltransferase, which is involved in the final steps of ergosterol biosynthesis. Our previous results showed that the Cgerg6Δ mutant has a lower ergosterol content in its membranes. Here, we show that the Cgerg6Δ mutant displays increased susceptibility to oxidative stress inducing agents, such as menadione, hydrogen peroxide and diamide, accompanied with increased intracellular ROS production. The Cgerg6Δ mutant is not able to tolerate higher concentrations of iron in the growth media. We observed increased expression of transcription factors, CgYap1p, CgMsn4p and CgYap5p, together with increased expression of catalase encoding the CgCTA1 gene and vacuolar iron transporter CgCCC1 in the Cgerg6Δ mutant cells. However, it seems that the CgERG6 gene deletion does not influence the function of mitochondria.
Ulam–Hyers Stability and Well-Posedness of Fixed Point Problems in C*-Algebra Valued Bipolar b-Metric Spaces
Authors: Kumar Manoj , Kumar Pankaj , Mutlu Ali , Ramaswamy Rajagopalan , Abdelnaby Ola A. Ashour , Radenović Stojan
Published: May 2023

Here, we shall introduce the new notion of C*-algebra valued bipolar b-metric spaces as a generalization of usual metric spaces, C*-algebra valued metric space, b-metric spaces. In the above-mentioned spaces, we shall define (αA−ψA) contractions and prove some fixed point theorems for these contractions. Some existing results from the literature are also proved by using our main results. As an application Ulam–Hyers stability and well-posedness of fixed point problems are also discussed. Some examples are also given to illustrate our results.
Cloud Services User’s Recommendation System Using Random Iterative Fuzzy-Based Trust Computation and Support Vector Regression
Authors: Ramesh Janjhyam Venkata Naga , Khasim Syed , Abbas Mohamed , Shaik Kareemulla , Rahman Mohammad Zia Ur , Elangovan Muniyandy
Published: May 2023

Cloud computing is now a fundamental type of computing due to technological innovation and it is believed to be a benefit for mid-scale enterprises. The use of cloud computing is increasing daily, which improves service quality but also gives rise to security concerns. Finding trustworthy service can be very challenging, take a great deal of time, or produce subpar services. Due to these difficulties, the client needs a service that is dependable, suitable, time-saving, and trustworthy. As a result, from the end user’s perspective, adopting a cloud service’s trustworthiness becomes crucial. Trust is a measure of how well users’ expectations about a service’s capabilities are realized. In this research, a recommendation system for cloud service customers based on random iterative fuzzy computation (RIFTC) is proposed. RIFTC focuses on the assessment of trust using Quality of Service (QoS) characteristics. RIFTC calculates trust using the machine learning approach Support Vector Regression (SVR). RIFTC can helpfully recommend a cloud service to the end user and anticipate the trust values of cloud services.. Precision (97%), latency (51%), throughput (25.99 mbps), mean absolute error (54%), and re-call (97%) rates are used to assess how well this recommendation system performs. RIFTC’s average F-measure rate is calculated by adjusting the number of users from 200 to 300, and it is 93.46% more accurate on average with less time spent than the current methodologies.
Complete Intradural Interbody Cage Migration in Lumbar Spine Surgery: A Case Report and Literature Review
Authors: Hsiao Pang-Hsuan , Lin Erh-Ti , Chen Hsien-Te , Lo Yuan-Shun
Published: May 2023

Background: Spinal fusion is a common surgery, in which vertebrae are fused to restore spinal stability and eliminate pain during movement. The use of an interbody cage facilitates spinal fusion. However, complete cage migration into the dura matter rarely occurs and can be challenging to manage. Case Presentation: A 44-year-old man presented at our spine center with a history of incomplete paraplegia and cauda equina syndrome that had lasted for 2 years and 4 months. This condition developed after he underwent six lumbar spine surgeries to address lower back pain and right-sided sciatica. A structural allograft kidney-shaped cage was found completely within the dura at the level of the L3 vertebra. Durotomy, cage retrieval, and pedicle screw fixation from the L2 to L4 vertebrae were performed. Numbness in both lower limbs markedly decreased within several days of the operation. After four months following the progressive physical therapy, the patient could partially control both urination and defecation. Five months postoperatively, he could stand with slight assistance. Conclusions: Complete intradural cage migration is a rare and serious complication. To the best of our knowledge, this is the first reported case with such a condition in the literature. Even if treatment is delayed, surgical intervention may salvage the remaining neurologic function and may even lead to partial recovery.
Innovations and Challenges in Breast Cancer Care: A Review
Authors: Chopra Sharat , Khosla Muskaan , Vidya Raghavan
Published: May 2023

Breast cancer care has seen tremendous advancements in recent years through various innovations to improve early detection, diagnosis, treatment, and survival. These innovations include advancements in imaging techniques, minimally invasive surgical techniques, targeted therapies and personalized medicine, radiation therapy, and multidisciplinary care. It is essential to recognize that challenges and limitations exist while significant advancements in breast cancer care exist. Continued research, advocacy, and efforts to address these challenges are necessary to make these innovations accessible to all patients while carefully considering and managing the ethical, social, and practical implications.
Advanced Trauma Operative Management in Children
Authors: Canavese Federico
Published: May 2023

Fractures in children and adolescents present a diagnostic and therapeutic challenge to the orthopedic surgeon as there are still many uncertainties in the scientific understanding of these injuries [...]
The Diagnosis, Pathophysiology, and Treatment of Chronic Hepatitis E Virus Infection—A Condition Affecting Immunocompromised Patients
Authors: Takakusagi Satoshi , Kakizaki Satoru , Takagi Hitoshi
Published: May 2023

Hepatitis E is a zoonosis caused by hepatitis E virus (HEV), which was first discovered 40 years ago. Twenty million HEV infections worldwide are estimated each year. Most hepatitis E cases are self-limiting acute hepatitis, but the virus has been recognized to cause chronic hepatitis. Following the first case report of chronic hepatitis E (CHE) in a transplant recipient, CHE has recently been identified as associated with chronic liver damage induced by HEV genotypes 3, 4, and 7—usually in immunocompromised patients such as transplant recipients. In addition, patients infected with HIV and those receiving chemotherapy for malignancy, along with patients with rheumatic disease and COVID-19, have recently been reported as having CHE. CHE can be easily misdiagnosed by usual diagnostic methods of antibody response, such as anti-HEV IgM or IgA, because of the low antibody response in the immunosuppressive condition. HEV RNA should be evaluated in these patients, and appropriate treatments—such as ribavirin—should be given to prevent progression to liver cirrhosis or liver failure. While still rare, cases of CHE in immunocompetent patients have been reported, and care must be taken not to overlook these instances. Herein, we conduct an overview of hepatitis E, including recent research developments and management of CHE, in order to improve our understanding of such cases. The early diagnosis and treatment of CHE should be performed to decrease instances of hepatitis-virus-related deaths around the world.
Isolation, Identification, and Characterization of Aeromonas veronii from Chinese Soft-Shelled Turtle (Trionyx sinensis)
Authors: Hu Xiaowei , Xiao Zidong , Li Bo , Xue Mingyang , Jiang Nan , Fan Yuding , Chen Peng , Qi Feng , Kong Xianghui , Zhou Yong
Published: May 2023

Aeromonas veronii is widespread in aquatic environments and is capable of infecting various aquatic organisms. A. veronii infection is lethal for Chinese soft-shelled turtles (Trionyx sinensis, CSST). We isolated a gram-negative bacterium from the liver of diseased CSSTs, which was named XC-1908. This isolate was identified as A. veronii based on its morphological and biochemical characteristics, and 16S rRNA gene sequence analysis. A. veronii was pathogenic for CSSTs with an LD50 of 4.17 × 105 CFU/g. The symptoms of CSSTs artificially infected with isolate XC-1908 were consistent with those of the naturally infected CSSTs. The levels of total protein, albumin, and white globule in the serum samples of the diseased turtles were decreased, whereas those of aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase were elevated. Moreover, the diseased CSSTs exhibited the following histopathological changes: the liver contained numerous melanomacrophage centers, renal glomerulus were edematous, intestinal villi were shed, and in oocytes, the number of vacuoles increased and red-rounded particles were observed. Antibiotic sensitivity tests revealed that the bacterium was sensitive to ceftriaxone, doxycycline, florfenicol, cefradine, and gentamicin, and resistant to sulfanilamide, carbenicillin, benzathine, clindamycin, erythromycin, and streptomycin. This study provides control strategies to prevent outbreaks of A. veronii infection in CSSTs.
Influence of a Composite Polylysine-Polydopamine-Quaternary Ammonium Salt Coating on Titanium on Its Ostogenic and Antibacterial Performance
Authors: Xing Lei , Song Hongyang , Wei Jinjian , Wang Xue , Yang Yaozhen , Zhe Pengbo , Luan Mingming , Xu Jing
Published: May 2023

Thin oxide layers form easily on the surfaces of titanium (Ti) components, with thicknesses of <100 nm. These layers have excellent corrosion resistance and good biocompatibility. Ti is susceptible to bacterial development on its surface when used as an implant material, which reduces the biocompatibility between the implant and the bone tissue, resulting in reduced osseointegration. In the present study, Ti specimens were surface-negatively ionized using a hot alkali activation method, after which polylysine and polydopamine layers were deposited on them using a layer-by-layer self-assembly method, then a quaternary ammonium salt (QAS) (EPTAC, DEQAS, MPA-N+) was grafted onto the surface of the coating. In all, 17 such composite coatings were prepared. Against Escherichia coli and Staphylococcus aureus, the bacteriostatic rates of the coated specimens were 97.6 ± 2.0% and 98.4 ± 1.0%, respectively. Thus, this composite coating has the potential to increase the osseointegration and antibacterial performance of implantable Ti devices.
Optimization of Phytochemical-Rich Citrus maxima Albedo Extract Using Response Surface Methodology
Authors: Inthachat Woorawee , Temviriyanukul Piya , On-Nom Nattira , Kanoongon Panyaporn , Thangsiri Sirinapa , Chupeerach Chaowanee , Suttisansanee Uthaiwan
Published: May 2023

In the present study, response surface methodology (RSM) and Box–Behnken design (BBD) were employed to optimize the conditions for the extraction of C. maxima albedo from agricultural waste, to obtain notable phytochemicals. Ethanol concentration, extraction temperature, and extraction time were included as key factors contributing to the extraction. The results showed that the optimum extraction condition for C. maxima albedo was 50% (v/v) aqueous ethanol at 30 °C for 4 h, which provided total phenolic contents and total flavonoid contents at 15.79 mg of gallic equivalent/g dry weight (DW) and 4.50 mg of quercetin equivalent/g DW, respectively. Considerable amounts of hesperidin and naringenin at 161.03 and 3430.41 µg/g DW, respectively, were detected in the optimized extract using liquid chromatography–electrospray ionization–tandem mass spectrometry (LC–ESI–MS/MS). The extract was later subjected to a test for its enzyme-inhibitory activities against key enzymes relevant to Alzheimer’s disease (AD), obesity, and diabetes as well as for its mutagenicity potential. Among enzyme inhibitory activities, the extract showed the highest inhibitory strength against β-secretase (BACE-1), which is a drug target for AD treatment. The extract was also devoid of mutagenicity properties. Overall, this study demonstrated a simple and optimal extraction procedure for C. maxima albedo with a significant quantity of phytochemicals, health benefits, and genome safety.
Could Selenium Supplementation Prevent COVID-19? A Comprehensive Review of Available Studies
Authors: Ambra Roberto , Melloni Sahara , Venneria Eugenia
Published: May 2023

The purpose of this review is to systematically examine the scientific evidence investigating selenium’s relationship with COVID-19, aiming to support, or refute, the growing hypothesis that supplementation could prevent COVID-19 etiopathogenesis. In fact, immediately after the beginning of the COVID-19 pandemic, several speculative reviews suggested that selenium supplementation in the general population could act as a silver bullet to limit or even prevent the disease. Instead, a deep reading of the scientific reports on selenium and COVID-19 that are available to date supports neither the specific role of selenium in COVID-19 severity, nor the role of its supplementation in the prevention disease onset, nor its etiology.
Wheat Oxylipins in Response to Aphids, CO2 and Nitrogen Regimes
Authors: Cascant-Vilaplana Mari Merce , Viteritti Eduardo , Sadras Víctor , Medina Sonia , Sánchez-Iglesias María Puerto , Oger Camille , Galano Jean-Marie , Durand Thierry , Gabaldón José Antonio , Taylor Julian , Ferreres Federico , Sergi Manuel , Gil-Izquierdo Angel
Published: May 2023

Wheat is critical for food security, and is challenged by biotic stresses, chiefly aphids and the viruses they transmit. The objective of this study was to determine whether aphids feeding on wheat could trigger a defensive plant reaction to oxidative stress that involved plant oxylipins. Plants were grown in chambers with a factorial combination of two nitrogen rates (100% N vs. 20% N in Hoagland solution), and two concentrations of CO2 (400 vs. 700 ppm). The seedlings were challenged with Rhopalosiphum padi or Sitobion avenae for 8 h. Wheat leaves produced phytoprostanes (PhytoPs) of the F1 series, and three types of phytofurans (PhytoFs): ent-16(RS)-13-epi-ST-Δ14-9-PhytoF, ent-16(RS)-9-epi-ST-Δ14-10-PhytoF and ent-9(RS)-12-epi-ST-Δ10-13-PhytoF. The oxylipin levels varied with aphids, but not with other experimental sources of variation. Both Rhopalosiphum padi and Sitobion avenae reduced the concentrations of ent-16(RS)-13-epi-ST-Δ14-9-PhytoF and ent-16(RS)-9-epi-ST-Δ14-10-PhytoF in relation to controls, but had little or no effect on PhytoPs. Our results are consistent with aphids affecting the levels of PUFAs (oxylipin precursors), which decreased the levels of PhytoFs in wheat leaves. Therefore, PhytoFs could be postulated as an early indicator of aphid hosting for this plant species. This is the first report on the quantification of non-enzymatic PhytoFs and PhytoPs in wheat leaves in response to aphids.
The Role of Lactylation in Mental Illness: Emphasis on Microglia
Authors: Sfera Adonis , Klein Carolina , Anton Johnathan J. , Kozlakidis Zisis , Andronescu Christina V.
Published: May 2023

A paradigm shift is currently taking place in the etiopathogenesis of neuropsychiatric disorders as immunometabolism is replacing the earlier neurotransmitter model. According to the new concept, cellular bioenergetics drives information processing in the central nervous system; therefore, neuropathology is conceptualized as a direct consequence of impaired metabolism. Along the same lines, endoplasmic reticulum stress and gut barrier dysfunction are emerging as novel targets in schizophrenia and affective disorders, linking immune responses to cellular distress. Furthermore, microglia, the brain’s innate immune cells, acquire energy through oxidative phosphorylation, while in the resting state, and glycolysis upon activation, contributing to lactate accumulation and reduced brain pH. The same metabolic signature characterizes neuropsychiatric disorders as the central nervous system derives adenosine triphosphate from aerobic glycolysis, upregulating lactate and generating an acidic environment. Although known for over three decades, the link between dysmetabolism and neuropathology was poorly defined until the discovery of brain-resident innate lymphoid cells, including natural killer cells, and lactylation of histone and nonhistone proteins. In this perspective article, we examine three anti-inflammatory microglial systems relevant for neuropsychiatry: lactate, oxytocin, and the aryl hydrocarbon receptor. We also discuss potential interventions for restoring microglial homeostasis.