bluuebunny/crossref_metadata_2025 huggingface dataset:
Percutaneous Left Atrial Appendage Closure in Patients with Non-Valvular Atrial Fibrillation and End-Stage Renal Disease on Hemodialysis: A Case Series
Authors: Basabe Elena , De La Flor José C. , López de la Manzanara Virginia , Nombela-Franco Luis , Narváez-Mejía Carlos , Cruzado Leónidas , Villa Daniel , Zamora Rocío , Tapia Manuel , Sastre Miguel Ángel , López Soberón Edurne , Herrero Calvo José A. , Suárez Alfonso , Martí Sánchez David
Published: January 2024

Non-valvular atrial fibrillation (NVAF) is the most common cardiac arrhythmia in the general population, and its prevalence increases among patients with chronic kidney disease (CKD) undergoing hemodialysis. This population presents high risk of both hemorrhagic and thrombotic events, with little evidence regarding the use of oral anticoagulation treatment (OAT) and multiple complications arising from it; however, stroke prevention with percutaneous left atrial appendage closure (LAAC) is an alternative to be considered. We retrospectively describe the safety and efficacy of percutaneous LAAC in eight patients with NVAF and CKD on hemodialysis during a 12-month follow-up. The mean age was 78.8 years (range 64–86; SD ± 6.7), and seven patients were male. The mean CHA2DS2-VASC and HAS-BLED scores were high, 4.8 (SD ± 1.5) and 3.8 (SD ± 1.3), respectively. Seventy-five percent of the patients were referred for this intervention due to a history of major bleeding, with gastrointestinal bleeding being the most common type, while the remaining twenty-five percent of the patients were referred because of a high risk of bleeding. The percutaneous LAAC procedure was successfully completed in 100% of the patients, with complete exclusion of the appendage without complications or leaks exceeding 5 mm. There was one death not related to the procedure four days after the intervention. Among the other seven patients, no deaths, cardioembolic events or major bleeding were reported during the follow-up period. In our sample, percutaneous LAAC appears to be a safe and effective alternative to anticoagulation in patients with NVAF and CKD on hemodialysis.
Ultra-Stable Inorganic Mesoporous Membranes for Water Purification
Authors: Bauer Ralph A. , Qiu Minghui , Schillo-Armstrong Melissa C. , Snider Matthew T. , Yang Zi , Zhou Yi , Verweij Hendrik
Published: January 2024

Thin, supported inorganic mesoporous membranes are used for the removal of salts, small molecules (PFAS, dyes, and polyanions) and particulate species (oil droplets) from aqueous sources with high flux and selectivity. Nanofiltration membranes can reject simple salts with 80–100% selectivity through a space charge mechanism. Rejection by size selectivity can be near 100% since the membranes can have a very narrow size distribution. Mesoporous membranes have received particular interest due to their (potential) stability under operational conditions and during defouling operations. More recently, membranes with extreme stability became interesting with the advent of in situ fouling mitigation by means of ultrasound emitted from within the membrane structure. For this reason, we explored the stability of available and new membranes with accelerated lifetime tests in aqueous solutions at various temperatures and pH values. Of the available ceria, titania, and magnetite membranes, none were actually stable under all test conditions. In earlier work, it was established that mesoporous alumina membranes have very poor stability. A new nanofiltration membrane was made of cubic zirconia membranes that exhibited near-perfect stability. A new ultrafiltration membrane was made of amorphous silica that was fully stable in ultrapure water at 80 °C. This work provides details of membrane synthesis, stability characterization and data and their interpretation.
Numerical Simulation Research on the Temperature Field and Hot Roll Crown Model of Hot Continuous Rolling Mills
Authors: Li Zizheng , Elmi Sahal Ahmed , Liu Luxuan , Yin Baoliang , Kuang Shuang , Bai Zhenhua
Published: January 2024

Addressing the challenge of roll loss and strip deformation arising from the lack of precise prediction of the roll temperature field in hot tandem rolling mills, this study employs numerical analysis via the finite difference method. Based on the roll temperature field and hot roll crown model, an intelligent support cooling control system for the roll cooling water of hot rolls is established. This system comprehensively considers the direct impact of specific parameters on the roll temperature field in the intricate context of cooling water dynamics. The study focuses on the cyclic superposition effect of rolling coil quantities on the roll temperature field and the resulting hot roll shape, and theoretical calculations along with simulation analyses were conducted using finite element software. Through the integration of field-measured values, the study achieves accurate predictions of the temperature field and hot roll profile for both work rolls and backup rolls.
A New Phenomenological Model to Predict Forming Limit Curves from Tensile Properties for Hot-Rolled Steel Sheets
Authors: Chen Wei-Jin , Song Hong-Wu , Chen Shuai-Feng , Xu Yong , Deng Si-Ying , Cai Zheng , Pei Xin-Hua , Zhang Shi-Hong
Published: January 2024

A phenomenological model for the prediction of the forming limit curve (FLC) based on basic mechanical properties through a uniaxial tensile test can tremendously shorten the design time of the forming process and reduce the measuring costs. In this paper, a novel phenomenological model named the IMR-Baosteel model (abbreviated as the IB model) is proposed for efficient and accurate FLC prediction of hot-rolled steel sheets featuring distinct variations in thickness and mechanical properties. With a systematic test of the plane strain forming limit (FLC0), it was found that a higher regression correlation exists between the FLC0 and the total elongation under different sheet thicknesses. For accurate assessment of the FLC0 from tensile properties, compared using experiments, the error of FLC0 calculated with the proposed model is within 10%. In the IB model, the left side of FLC can be calculated using a line with a slope of −1 while the right side of the FLC is obtained via a modified Keeler model with the exponent (p) determined as 0.45 for hot-rolled steels. Complete experimental FLCs of hot-rolled steels from measurements and the literature were used to validate the reliability of the proposed model. Resultantly, the prediction of FLCs with the proposed IB model is greatly improved, and agrees much better with the experimental FLCs than the predictions of the well-known Keeler model, Arcelor model and Tata Steel model.
Improved Performance of Acoustically Actuated Magnetoelectric Antenna with FeGa/FeGaB Bilayer
Authors: Li Kunqi , Zhang Qiaozhen , Chang Yang , Wang Jian , Liu Huiling , Zhang Songsong , Gu Yuandong
Published: January 2024

Acoustically actuated magnetoelectric (ME) antennas utilize acoustic wave resonance to complete the process of receiving and transmitting signals, which promotes the development of antenna miniaturization technology. This paper presents a bilayer magnetostrictive/AlN ME laminated antenna. The proposed laminated antenna uses the FeGa/FeGaB bilayer materials as magnetostrictive materials, which combine the advantages of soft magnetic properties of FeGa and the low loss of FeGaB. First, multiphysics modeling and analysis are performed for the proposed ME laminated antenna by finite element method (FEM). The positive/inverse ME effects and the influences of the volume ratio of the FeGa/FeGaB bilayer on the antenna performance are studied. The results show that the output voltage and ME coefficient of the FeGa/FeGaB bilayer magnetostrictive material with a volume ratio of 1:1 are 3.97 times and 195.8% higher than that of the single FeGaB layer, respectively. The eddy current loss is 52.08% lower than that of single-layer FeGa. According to the surface equivalence principle, the far-field radiation process is simulated. The results show that the gain of the ME antenna is 15 dB larger than that of the same-size micro-loop antenna, and the gain of the ME antenna is about −44.9 dB. The improved performance and magnetic tunability of the proposed bilayer magnetostrictive materials make ME antennas excellent candidates for portable devices and implantable medical devices.
Simulation Study on the Charge Collection Mechanism of FinFET Devices in Single-Event Upset
Authors: Zhang Hongwei , Guo Yang , Wang Shida , Sun Yi , Mei Bo , Tang Min , Liu Jingyi
Published: January 2024

Planar devices and FinFET devices exhibit significant differences in single-event upset (SEU) response and charge collection. However, the charge collection process during SEU in FinFET devices has not been thoroughly investigated. This article addresses this gap by establishing a FinFET SRAM simulation structure and employing simulation software to delve into the charge collection process of FinFET devices during single-event upset. The results reveal substantial differences in charge collection between NMOS and PMOS, and that direct incidence of PMOS leads to the phenomenon of multiple-node charge collection causing SRAM unit upset followed by recovery.
Effects of Nutrition on Maternal Health, Fetal Development, and Perinatal Outcomes
Authors: Apostolopoulou Aikaterini , Tranidou Antigoni , Tsakiridis Ioannis , Magriplis Emmanuella , Dagklis Themistoklis , Chourdakis Michail
Published: January 2024

The early life theory states that the first 1000 days of a person’s life are highly influential, as lasting health impacts can be attained during this period [...]
Dietary Patterns and Hypothyroidism in U.S. Adult Population
Authors: Alkhatib Dana , Shi Zumin , Ganji Vijay
Published: January 2024

The thyroid gland produces hormones that are essential for various body functions. Hypothyroidism is defined as insufficient thyroid hormone production. Several studies have found associations between specific micronutrients and overall thyroid function; however, the amount of evidence regarding the relationship between dietary patterns and hypothyroidism among the U.S. population is limited. Data from three cycles of National Health and Nutrition Examination Surveys (NHANES), 2007–2008, 2009–2010, and 2011–2012, were used (n = 8317). Subjects with serum thyroid stimulating hormone >4.5 mIU/L or on levothyroxine were considered to have hypothyroidism. Age, sex, race/ethnicity, body mass index, and several lifestyle factors were considered as covariates. Three patterns were extracted using factor analysis. These were labeled as fat–processed grains–sugars–meats (FPSM), oils–nuts–potatoes–low-fat meats (ONPL), and fruits–whole grains–vegetables–dairy (FWVD) patterns. In a weighted multiple logistic regression, FPSM and ONPL were inversely associated with hypothyroidism (OR, 0.75; 95% CI, 0.57–1; p = 0.049 and OR, 0.81; 95% CI, 0.67–0.97; p = 0.025, respectively). However, FWVD demonstrated no association with hypothyroidism (p = 0.63). In conclusion, FPSM and ONPL patterns but not FWVD patterns were associated with hypothyroidism in U.S. adults. Nutrient deficiencies and their interactions may be linked to hypothyroidism.
Vitamin C Inhibits Lipopolysaccharide-Induced Hyperinflammatory State of Chronic Myeloid Leukemia Cells through Purinergic Signaling and Autophagy
Authors: Pires Daniela A. , Brandão-Rangel Maysa A. R. , Silva-Reis Anamei , Olímpio Fabiana R. S. , Aimbire Flavio , Oliveira Carlos R. , Mateus-Silva José R. , Zamarioli Lucas S. , Bachi André L. L. , Bella Yanesko F. , Santos Juliana M. B. , Bincoletto Claudia , Lancha Antonio Herbert , Vieira Rodolfo P.
Published: January 2024

Background: Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm characterized by the overproduction of white blood cells, leading to symptoms such as fatigue, infections, and other complications. CML patients must take measures to prevent infections to mitigate the exacerbation of cancer cell proliferation and comorbidities. Methods: This study investigated whether vitamin C can suppress the hyperinflammatory activation of K-562 cells induced by lipopolysaccharide (LPS) and whether purinergic signaling (ATP and P2X7 receptor) and autophagy play a role in it. Two different doses of vitamin C (5 µg/mL and 10 µg/mL) were employed, along with the lysosome inhibitor chloroquine (CQ; 100 µM), administered 2 h prior to LPS stimulation (10 ng/mL) for a duration of 22 h in K-562 cells (3 × 105 cells/mL/well). Results: Both doses of vitamin C reduced the release of interleukin-6 (IL-6) (5 µg/mL, p < 0.01 and 10 µg/mL, p < 0.01) and tumor necrosis factor (TNF) (5 µg/mL, p < 0.01 and 10 µg/mL, p < 0.01) induced by LPS. Furthermore, in LPS + CQ-stimulated cells, vitamin C at a concentration of 10 µg/mL inhibited the expression of LC3-II (p < 0.05). Conversely, both doses of vitamin C led to the release of the anti-inflammatory cytokine interleukin-10 (IL-10) (5 µg/mL, p < 0.01 and 10 µg/mL, p < 0.01), while only the 10 µg/mL dose of vitamin C induced the release of Klotho (10 µg/mL, p < 0.01). In addition, both doses of vitamin C reduced the accumulation of ATP (5 µg/mL, p < 0.01 and 10 µg/mL, p < 0.01) and decreased the expression of the P2X7 receptor at the mRNA level. Conclusions: Vitamin C inhibits the hyperinflammatory state induced by LPS in K-562 cells, primarily by inhibiting the ATP accumulation, P2X7 receptor expression, and autophagy signaling.
Preoperative Body Composition Correlates with Postoperative Muscle Volume and Degeneration after Total Hip Arthroplasty
Authors: Ukai Taku , Yokoyama Katsuya , Watanabe Masahiko
Published: January 2024

Impaired muscle recovery after total hip arthroplasty (THA) may affect gait and activities of daily living. Bioelectrical impedance analysis (BIA) can assess body composition and muscle volume, and computed tomography (CT) can assess muscle volume and the fatty degeneration of muscle. This study aimed to explore the effectiveness of BIA, and the correlation between preoperative body composition and postoperative muscle volume and degeneration after THA using BIA and CT. Thirty-eight patients who underwent THA and had BIA and CT performed pre- and postoperatively were retrospectively assessed. The BIA-derived measurements of preoperative body composition (fat mass index, fat-free mass index, and phase angle) were correlated with the CT-derived measurements (pre- and postoperative muscle volume and gluteus maximus and quadriceps Hounsfield Units of the affected hip). The preoperative fat mass index negatively correlated with the postoperative muscle volume of the gluteus maximus (p = 0.02) and quadriceps (p < 0.001) and the Hounsfield Units of the gluteus maximus (p = 0.03) and quadriceps (p = 0.03). The preoperative fat-free mass index positively correlated with the postoperative muscle volume of the quadriceps (p = 0.02). The preoperative phase angle positively correlated with the postoperative muscle volume of the quadriceps (p = 0.001) and the Hounsfield Units of the gluteus maximus (p = 0.03) and quadriceps (p = 0.001). In patients who underwent THA, preoperative body composition correlated with postoperative muscle volume and the fatty degeneration of the affected lower limb. Preoperative body composition may help predict postoperative muscle volume and fatty degeneration and thus, postoperative recovery.
The Role of Mycoplasma bovirhinis in the Development of Singular and Concomitant Respiratory Infections in Dairy Calves from Southern Brazil
Authors: Frucchi Ana Paula Souza , Dall Agnol Alais Maria , Caldart Eloiza Teles , Bronkhorst Dalton Everton , Alfieri Alice Fernandes , Alfieri Amauri Alcindo , Headley Selwyn Arlington
Published: January 2024

The role of Mycoplasma bovirhinis in the development of pulmonary disease in cattle is controversial and was never evaluated in cattle from Latin America. This study investigated the respiratory infection dynamics associated with M. bovirhinis in suckling calves from 15 dairy cattle herds in Southern Brazil. Nasal swabs were obtained from asymptomatic (n = 102) and calves with clinical manifestations (n = 103) of bovine respiratory disease (BRD) and used in molecular assays to identify the specific genes of viral and bacterial disease pathogens of BRD. Only M. bovirhinis, bovine coronavirus (BCoV), ovine gammaherpesvirus 2 (OvGHV2), Histophilus somni, Pasteurella multocida, and Mannheimia haemolytica were detected. M. bovirhinis was the most frequently diagnosed pathogen in diseased (57.8%; 59/102) and asymptomatic (55.3%; 57/103) calves at all farms. BCoV-related infections were diagnosed in diseased (52%; 53/102) and asymptomatic (51.4%; 53/103) calves and occurred in 93.3% (14/15) of all farms. Similarly, infectious due to OvGHV2 occurred in diseased (37.2%; 38/102) and asymptomatic (27.2%; /28/103) calves and were diagnosed in 80% (12/15) of all farms investigated. Significant statistical differences were not identified when the two groups of calves were compared at most farms, except for infections due to OvGHV2 that affected five calves at one farm. These results demonstrated that the respiratory infection dynamics of M. bovirhinis identified in Southern Brazil are similar to those observed worldwide, suggesting that there is not enough sufficient collected data to consider M. bovirhinis as a pathogen of respiratory infections in cattle. Additionally, the possible roles of BCoV and OvGHV2 in the development of BRD are discussed.
The Dynamic Landscape of Capsid Proteins and Viral RNA Interactions in Flavivirus Genome Packaging and Virus Assembly
Authors: Jablunovsky Anastazia , Jose Joyce
Published: January 2024

The Flavivirus genus of the Flaviviridae family of enveloped single-stranded RNA viruses encompasses more than 70 members, many of which cause significant disease in humans and livestock. Packaging and assembly of the flavivirus RNA genome is essential for the formation of virions, which requires intricate coordination of genomic RNA, viral structural, and nonstructural proteins in association with virus-induced, modified endoplasmic reticulum (ER) membrane structures. The capsid (C) protein, a small but versatile RNA-binding protein, and the positive single-stranded RNA genome are at the heart of the elusive flavivirus assembly process. The nucleocapsid core, consisting of the genomic RNA encapsidated by C proteins, buds through the ER membrane, which contains viral glycoproteins prM and E organized as trimeric spikes into the lumen, forming an immature virus. During the maturation process, which involves the low pH-mediated structural rearrangement of prM and E and furin cleavage of prM in the secretory pathway, the spiky immature virus with a partially ordered nucleocapsid core becomes a smooth, mature virus with no discernible nucleocapsid. This review focuses on the mechanisms of genome packaging and assembly by examining the structural and functional aspects of C protein and viral RNA. We review the current lexicon of critical C protein features and evaluate interactions between C and genomic RNA in the context of assembly and throughout the life cycle.
Novel Thiourea and Oxime Ether Isosteviol-Based Anticoagulants: MD Simulation and ADMET Prediction
Authors: Gackowski Marcin , Jędrzejewski Mateusz , Medicharla Sri Satya , Kondabala Rajesh , Madriwala Burhanuddin , Mądra-Gackowska Katarzyna , Studzińska Renata
Published: January 2024

Activated blood coagulation factor X (FXa) plays a critical initiation step of the blood-coagulation pathway and is considered a desirable target for anticoagulant drug development. It is reversibly inhibited by nonvitamin K antagonist oral anticoagulants (NOACs) such as apixaban, betrixaban, edoxaban, and rivaroxaban. Thrombosis is extremely common and is one of the leading causes of death in developed countries. In previous studies, novel thiourea and oxime ether isosteviol derivatives as FXa inhibitors were designed through a combination of QSAR studies and molecular docking. In the present contribution, molecular dynamics (MD) simulations were performed for 100 ns to assess binding structures previously predicted by docking and furnish additional information. Moreover, three thiourea- and six oxime ether-designed isosteviol analogs were then examined for their drug-like and ADMET properties. MD simulations demonstrated that four out of the nine investigated isosteviol derivatives, i.e., one thiourea and three oxime ether ISV analogs, form stable complexes with FXa. These derivatives interact with FXa in a manner similar to Food and Drug Administration (FDA)-approved drugs like edoxaban and betrixaban, indicating their potential to inhibit factor Xa activity. One of these derivatives, E24, displays favorable pharmacokinetic properties, positioning it as the most promising drug candidate. This, along with the other three derivatives, can undergo further chemical synthesis and bioassessment.
Machine Learning Application for Medicinal Chemistry: Colchicine Case, New Structures, and Anticancer Activity Prediction
Authors: Nowak Damian , Huczyński Adam , Bachorz Rafał Adam , Hoffmann Marcin
Published: January 2024

In the contemporary era, the exploration of machine learning (ML) has gained widespread attention and is being leveraged to augment traditional methodologies in quantitative structure–activity relationship (QSAR) investigations. The principal objective of this research was to assess the anticancer potential of colchicine-based compounds across five distinct cell lines. This research endeavor ultimately sought to construct ML models proficient in forecasting anticancer activity as quantified by the IC50 value, while concurrently generating innovative colchicine-derived compounds. The resistance index (RI) is computed to evaluate the drug resistance exhibited by LoVo/DX cells relative to LoVo cancer cell lines. Meanwhile, the selectivity index (SI) is computed to determine the potential of a compound to demonstrate superior efficacy against tumor cells compared to its toxicity against normal cells, such as BALB/3T3. We introduce a novel ML system adept at recommending novel chemical structures predicated on known anticancer activity. Our investigation entailed the assessment of inhibitory capabilities across five cell lines, employing predictive models utilizing various algorithms, including random forest, decision tree, support vector machines, k-nearest neighbors, and multiple linear regression. The most proficient model, as determined by quality metrics, was employed to predict the anticancer activity of novel colchicine-based compounds. This methodological approach yielded the establishment of a library encompassing new colchicine-based compounds, each assigned an IC50 value. Additionally, this study resulted in the development of a validated predictive model, capable of reasonably estimating IC50 values based on molecular structure input.
In Vivo Deposition of High-Flow Nasal Aerosols Using Breath-Enhanced Nebulization
Authors: Jayakumaran Jeyanthan , Smaldone Gerald C.
Published: January 2024

Aerosol delivery using conventional nebulizers with fixed maximal output rates is limited and unpredictable under high-flow conditions. This study measured regulated aerosol delivery to the lungs of normal volunteers using a nebulizer designed to overcome the limitations of HFNC therapy (i-AIRE (InspiRx, Inc., Somerset, NJ, USA)). This breath-enhanced jet nebulizer, in series with the high-flow catheter, utilizes the high flow to increase aerosol output beyond those of conventional devices. Nine normal subjects breathing tidally via the nose received humidified air at 60 L/min. The nebulizer was connected to the HFNC system upstream to the humidifier and received radio-labeled saline as a marker for drug delivery (99mTc DTPA) infused by a syringe pump (mCi/min). The dose to the subject was regulated at 12, 20 and 50 mL/h. Rates of aerosol deposition in the lungs (µCi/min) were measured via a gamma camera for each infusion rate and converted to µg NaCl/min. The deposition rate, as expressed as µg of NaCl/min, was closely related to the infusion rate: 7.84 ± 3.2 at 12 mL/h, 43.0 ± 12 at 20 mL/h and 136 ± 45 at 50 mL/h. The deposition efficiency ranged from 0.44 to 1.82% of infused saline, with 6% deposited in the nose. A regional analysis indicated peripheral deposition of aerosol (central/peripheral ratio 0.99 ± 0.27). The data were independent of breathing frequency. Breath-enhanced nebulization via HFNC reliably delivered aerosol to the lungs at the highest nasal airflows. The rate of delivery was controlled simply by regulating the infusion rate, indicating that lung deposition in the critically ill can be titrated clinically at the bedside.
Formulation and Preparation of Losartan-Potassium-Loaded Controlled-Release Matrices Using Ethocel Grade 10 to Establish a Correlation between In Vitro and In Vivo Results
Authors: Khan Kamran Ahmad , Ahmad Ashfaq , Marini Carlotta , Nicotra Mario , Di Cerbo Alessandro , Fazal-Ur-Rehman  , Ullah Naveed , Khan Gul Majid
Published: January 2024

In the current study, matrices of losartan potassium were formulated with two different polymers (Ethocel 10 premium and Ethocel 10FP premium), along with a filler and a lubricant, at different drug-to-polymer w/w ratios (10:3, 10:4, and 10:5). The matrices were tested by the direct compression method, and their hardness, diameter, thickness, friability, weight variation, content uniformity, and in vitro dissolution tests were assessed to determine 24-h drug release rates. The matrices with Ethocel 10 FP at a 10:4 ratio exhibited pseudo-zero-order kinetics (n-value of 0.986), while the dissolution data of the test matrices and reference tablets did not match. The new test-optimized matrices were also tested in rabbits, and their pharmacokinetic parameters were investigated: half-life (11.78 ± 0.018 h), Tmax (2.105 ± 1.131 h), Cmax (205.98 ± 0.321 μg/mL), AUCo (5931.10 ± 1.232 μg·h/mL), AUCo-inf (7348.46 ± 0.234 μg·h/mL), MRTo-48h (17.34 ± 0.184 h), and Cl (0.002 ± 0.134 mL/min). A correlation value of 0.985 between the in vitro and in vivo results observed for the test-optimized matrices was observed, indicating a level-A correlation between the percentage of the drug released in vitro and the percentage of the drug absorbed in vivo. The matrices might improve patient compliance with once-a-day dosing and therapeutic outcomes.
RETRACTED: Fahmy et al. Intranasal Niosomal In Situ Gel as a Promising Approach for Enhancing Flibanserin Bioavailability and Brain Delivery: In Vitro Optimization and Ex Vivo/In Vivo Evaluation. Pharmaceutics 2020, 12, 485
Authors: Fahmy Usama A. , Badr-Eldin Shaimaa M. , Ahmed Osama A. A. , Aldawsari Hibah M. , Tima Singkome , Asfour Hani Z. , Al-Rabia Mohammed W. , Negm Aya A. , Sultan Muhammad H. , Madkhali Osama A. A. , Alhakamy Nabil A.
Published: January 2024

This journal retracts the article “Intranasal Niosomal In Situ Gel as a Promising Approach for Enhancing Flibanserin Bioavailability and Brain Delivery: In Vitro Optimization and Ex Vivo/In Vivo Evaluation” [...]
RETRACTED: Awan et al. The Enhanced Cytotoxic and Pro-Apoptotic Effects of Optimized Simvastatin-Loaded Emulsomes on MCF-7 Breast Cancer Cells. Pharmaceutics 2020, 12, 597
Authors: Awan Zuhier A. , Fahmy Usama A. , Badr-Eldin Shaimaa M. , Ibrahim Tarek S. , Asfour Hani Z. , Al-Rabia Mohammed W. , Alfarsi Anas , Alhakamy Nabil A. , Abdulaal Wesam H. , Al Sadoun Hadeel , Helmi Nawal , Noor Ahmad O. , Caraci Filippo , Almasri Diena M. , Caruso Giuseppe
Published: January 2024

The journal retracts the article, “The Enhanced Cytotoxic and Pro-Apoptotic Effects of Optimized Simvastatin-Loaded Emulsomes on MCF-7 Breast Cancer Cells” [...]
RETRACTED: Ahmed et al. Omega-3 Self-Nanoemulsion Role in Gastroprotection against Indomethacin-Induced Gastric Injury in Rats. Pharmaceutics 2020, 12, 140
Authors: Ahmed Osama A. A. , Fahmy Usama A. , Bakhaidar Rana , El-Moselhy Mohamed A. , Okbazghi Solomon Z. , Ahmed Al-Shaimaa F. , Hammad Asmaa S. A. , Alhakamy Nabil A.
Published: January 2024

The journal retracts the article, “Omega-3 Self-Nanoemulsion Role in Gastroprotection against Indomethacin-Induced Gastric Injury in Rats” [...]
Melatonin Modulates Tomato Root Morphology by Regulating Key Genes and Endogenous Hormones
Authors: Tian Qiang , Wang Guangzheng , Dou Jianhua , Niu Yu , Li Ruirui , An Wangwang , Tang Zhongqi , Yu Jihua
Published: January 2024

Melatonin plays a vital role in plant growth and development. In this study, we treated hydroponically grown tomato roots with various concentrations of exogenous melatonin (0, 10, 30, and 50 μmol·L−1). We utilized root scanning and microscopy to examine alterations in root morphology and cell differentiation and elucidated the mechanism by which melatonin regulates these changes through the interplay with endogenous hormones and relevant genes. The results showed that for melatonin at concentrations ranging between 10 and 30 μmol·L−1, the development of lateral roots were significantly stimulated, the root hair growth was enhanced, and biomass accumulation and root activity were increased. Furthermore, we elucidated that melatonin acts as a mediator for the expression of genes, such as SlCDKA1, SlCYCA3;1, SlARF2, SlF3H, and SlKT1, which are involved in the regulation of root morphology changes. Additionally, we observed that melatonin influences the levels of endogenous hormones, including ZT, GA3, IAA, ABA, and BR, which subsequently impact the root morphology development of tomato roots. In summary, this study shows that tomato root morphology can be promoted by the optimal concentration of exogenous melatonin (10–30 μmol·L−1).
An Optimized and Cost-Effective RNA Extraction Method for Secondary Metabolite-Enriched Tissues of Norway Spruce (Picea abies)
Authors: Singh Vivek Vikram , Naseer Aisha , Sellamuthu Gothandapani , Jakuš Rastislav
Published: January 2024

Since the development of next-generation sequencing techniques and with the growing interest in transcriptomic studies, there is a demand for high-throughput RNA extraction techniques. General RNA extraction protocols are unreliable when it comes to the quality and quantity of isolated RNA obtained from different tissue types of different plant species. Despite Norway spruce (Picea abies) being one of the most significant and commercially valuable tree species in European forests, only limited genetic research is available. In this study, we developed a cetyltrimethylammonium bromide (CTAB) protocol by modifying the original method. We compared this CTAB protocol with other widely used methods for extracting RNA from different tissues (needle, phloem, and root) of Norway spruce, known for its richness in polyphenols, polysaccharides, and secondary metabolites. The modified CTAB method proves to be superior to the kit-based and TRIzol-based methods for extracting RNA from the metabolite-rich tissues of Norway spruce, resulting in high RNA quality and integrity values (RIN~7–9). The modified CTAB RNA extraction method is rapid, cost-effective, and relatively simple in yielding the desired RNA quality from Norway spruce tissues. It is optimal for RNA sequencing and other downstream molecular applications.
Ecotype-Specific and Correlated Seasonal Responses of Biomass Production, Non-Structural Carbohydrates, and Fatty Acids in Zostera marina
Authors: Beca-Carretero Pedro , Marín Clara , Azcárate-García Tomás , Cara Claudia L. , Brun Fernando , Stengel Dagmar B.
Published: January 2024

Seagrasses, which are marine flowering plants, provide numerous ecological services and goods. Zostera marina is the most widely distributed seagrass in temperate regions of the northern hemisphere, tolerant of a wide range of environmental conditions. This study aimed to (i) examine seasonal trends and correlations between key seagrass traits such as biomass production and biochemical composition, and (ii) compare seasonal adaptation of two ecotypes of Z. marina exposed to similar environmental conditions on the west coast of Ireland. During summer, plants accumulated higher levels of energetic compounds and levels of unsaturated fatty acids (FAs) decreased. Conversely, the opposite trend was observed during colder months. These findings indicate a positive seasonal correlation between the production of non-structural carbohydrates and saturated fatty acids (SFAs), suggesting that seagrasses accumulate and utilize both energetic compounds simultaneously during favorable and unfavorable environmental conditions. The two ecotypes displayed differential seasonal responses by adjusting plant morphology and production, the utilization of energetic reserves, and modulating unsaturation levels of fatty acids in seagrass leaves. These results underscore the correlated seasonal responses of key compounds, capturing ecotype-specific environmental adaptations and ecological strategies, emphasizing the robust utility of these traits as a valuable eco-physiological tool.
The In Vitro Inhibitory Activity of Pacaya Palm Rachis versus Dipeptidyl Peptidase-IV, Angiotensin-Converting Enzyme, α-Glucosidase and α-Amylase
Authors: Bernardino-Nicanor Aurea , Fernández-Avalos Stephanie , Juárez-Goiz José Mayolo Simitrio , Montañez-Soto José Luis , González-Cruz Leopoldo
Published: January 2024

The pacaya palm (Chamaedorea tepejilote Liebm) is an important food that is commonly consumed in Mexico and Central America due to its nutritive value. It is also used as a nutraceutical food against some chronic diseases, such as hypertension and hyperglycemia. However, few reports have indicated its possible potential. For this reason, the goal of this research was to evaluate the effects of the enzymatic activity of the pacaya palm inflorescence rachis on both hypertension and hyperglycemia and the effects of thermal treatments on the enzymatic activity. The enzymatic inhibition of ACE (angiotensin-converting enzyme), DPP-IV (dipeptidyl peptidase-IV), α-glucosidase and α-amylase were evaluated, all with powder extracts of pacaya palm inflorescences rachis. The results indicated that thermally treated rachis showed increased enzymatic inhibitory activity against α-amylase and DPP-IV. However, all rachis, both with and without thermal treatment, showed low- or no enzymatic activity against α-glucosidase and ACE. Apparently, the mechanism of action of the antidiabetic effect of rachis is mediated by the inhibition of α-amylase and DPP-IV and does not contribute with a significant effect on enzymes involved in the hypertension mechanism. Finally, the properties of the extract were modified via the extraction method and the temperature tested.
Pseudomonas taetrolens ULE-PH5 and Pseudomonas sp. ULE-PH6 Isolated from the Hop Rhizosphere Increase Phosphate Assimilation by the Plant
Authors: Ghoreshizadeh Seyedehtannaz , Calvo-Peña Carla , Ruiz-Muñoz Marina , Otero-Suárez Rebeca , Coque Juan José R. , Cobos Rebeca
Published: January 2024

Most of the phosphorus incorporated into agricultural soils through the use of fertilizers precipitates in the form of insoluble salts that are incapable of being used by plants. This insoluble phosphorus present in large quantities in soil forms the well-known “phosphorus legacy”. The solubilization of this “phosphorus legacy” has become a goal of great agronomic importance, and the use of phosphate-solubilizing bacteria would be a useful tool for this purpose. In this work, we have isolated and characterized phosphate-solubilizing bacteria from the rhizosphere of hop plants. Two particular strains, Pseudomonas taetrolens ULE-PH5 and Pseudomonas sp. ULE-PH6, were selected as plant growth-promoting rhizobacteria due to their high phosphate solubilization capability in both plate and liquid culture assays and other interesting traits, including auxin and siderophore production, phytate degradation, and acidic and alkaline phosphatase production. These strains were able to significantly increase phosphate uptake and accumulation of phosphorus in the aerial part (stems, petioles, and leaves) of hop plants, as determined by greenhouse trials. These strains are promising candidates to produce biofertilizers specifically to increase phosphate adsorption by hop plants.
Excellent Oxygen Barrier Property of Unfilled Natural Rubber/trans-Butadiene-co-Isoprene Rubber Vulcanizates under the Synergistic Effect of Crosslinking Density and Crystallization
Authors: Xia Pengcheng , Shao Huafeng , He Aihua
Published: January 2024

The thermo-oxidative aging of rubber products is inevitable during their use and leads to product failure and can even endanger safety. Oxygen is an important factor that cannot be ignored during the thermo-oxidative aging process. Thus, the gas barrier property of rubber products is of significant concern. In this work, a strategy of crystallizing rubber in unfilled rubber composites was designed by firstly constructing a dual synergistic mechanism of crosslinking density and crystallization on the oxygen barrier properties. As a crystallizable polymer, trans-butadiene-co-isoprene rubber (TBIR) shows dendritic fibril crystals or spherulites in natural rubber (NR)/TBIR vulcanizates. Meanwhile, the vulcanizates containing TBIR have a higher crosslinking density than NR vulcanizates. These TBIR-rich crystals and high-crosslinking-density structures are distributed in vulcanizates like continuous islands. Contrary to what has been reported in the literature, the decrease in oxygen permeability of NR/TBIR is not only due to the high crosslinking density and free volume of the polymer matrix, but more importantly, the spherulites of TBIR play a role in blocking and prolonging the oxygen diffusion path during the diffusion of oxygen in the polymer composites. We propose that the compatible crystalline polymer can replace the lamellar filler, play the role of the oxygen barrier in rubber composites, reduce the diffusion and dissolution of oxygen, and achieve the effect of improving the thermo-oxidative aging property of the rubber composite. Future research will follow the morphology evolution of TBIR crystals, their crosslinking structure and density, and interactions between TBIR and NR on the oxygen barrier and thermo-oxidative aging property.
Effect of Resin-Missing Defects on Tensile Behavior of Carbon Fiber/Epoxy Composites
Authors: Li Hongfeng , Li Feng , Zhu Lingxue
Published: January 2024

This study explores the impact of resin-missing defects on the mechanical properties of composite laminates through experimental and finite element methods. Specimens with varying defect contents (5.3%, 8.0%, 10.7%, 13.3%, and 16.7%) were prepared via Vacuum Assistant Resin Infusion process. Experimental tests were conducted with the assistance of Digital Image Correlation measurements to illustrate the impact of resin-missing defects on failure characteristics. The experimental results indicate that the existence of resin-missing defects altered the stress distribution, increased the local stress, and reduced the tensile strength of the composite laminate. The DIC results indicate that the presence of defects weakens the matrix, leading to premature damage and deterioration. Numerical modeling with a progressive damage analysis method was developed to simulate the failure process and the influence of the resin-missing defects. The simulation results agree well with the experimental results, and the maximum error was 3.06%. The failure modes obtained from finite elements are consistent with the experimental and DIC results. Furthermore, a study was conducted on how the location of resin-missing defects affects the mechanical properties of composite laminates. The findings suggest that defects situated at the edges or on the surface of the material have a more significant impact on the tensile strength.
Effect of Abiotic Treatments on Agricultural Plastic Waste: Efficiency of the Degradation Processes
Authors: Blesa Marco Zbigniew Emil , Sáez José Antonio , Andreu-Rodríguez Francisco Javier , Penalver Rosa , Rodríguez Manuel , Eissenberger Kristina , Cinelli Patrizia , Bustamante María Ángeles , Moral Raúl
Published: January 2024

In this study, four different plastic materials usually used in the agricultural sector (polystyrene film (PS), polyethylene terephthalate film (PET), low-density polyethylene film (LDPE) and linear low-density polyethylene film (LLDPE)) were subjected to different abiotic treatments, including photo-oxidation (ultraviolet and e-beam radiation) and thermochemical treatments, to enhance polymer degradation. The extensive use of these polymers leads to large amounts of plastic waste generation, including small plastic pieces, known as microplastics, which affect the quality of the agricultural environment, including soil fertility and quality. Therefore, polymer degradation strategies are needed to effectively reduce plastic waste to protect the agricultural sector. The degree of polymer degradation was assessed by the use of thermal and spectroscopic analyses, such as TGA and FTIR. In addition, efficiency, cost–benefits, and potential side-effects were also evaluated to propose the optimal degradation strategy to reduce plastic waste from the point of view of efficiency. The results obtained showed that the pre-treatments based on photo-oxidation (ultraviolet B and C and e-beam radiation) were more efficient and had a better cost–benefit for the degradation of the polymers studied in relation to the thermochemical treatments. Specifically, ultraviolet photo-oxidation worked well for PS and PET, requiring low energy and medium times. However, e-beam radiation was recommended for PE (LDPE and LLDPE) degradation, since high energy and long times were needed when ultraviolet energy was applied to this polymer. Furthermore, the overall efficiency of the plastic degradation of pre-treatments should be studied using a multicriteria approach, since FTIR assessments, in some cases, only consider oxidation processes on the plastic surface and do not show the potential integrity changes on the plastic probes.
Poly(methyl methacrylate) in Orthopedics: Strategies, Challenges, and Prospects in Bone Tissue Engineering
Authors: Ramanathan Susaritha , Lin Yu-Chien , Thirumurugan Senthilkumar , Hu Chih-Chien , Duann Yeh-Fang , Chung Ren-Jei
Published: January 2024

Poly(methyl methacrylate) (PMMA) is widely used in orthopedic applications, including bone cement in total joint replacement surgery, bone fillers, and bone substitutes due to its affordability, biocompatibility, and processability. However, the bone regeneration efficiency of PMMA is limited because of its lack of bioactivity, poor osseointegration, and non-degradability. The use of bone cement also has disadvantages such as methyl methacrylate (MMA) release and high exothermic temperature during the polymerization of PMMA, which can cause thermal necrosis. To address these problems, various strategies have been adopted, such as surface modification techniques and the incorporation of various bioactive agents and biopolymers into PMMA. In this review, the physicochemical properties and synthesis methods of PMMA are discussed, with a special focus on the utilization of various PMMA composites in bone tissue engineering. Additionally, the challenges involved in incorporating PMMA into regenerative medicine are discussed with suitable research findings with the intention of providing insightful advice to support its successful clinical applications.
Development and Validation of an HPLC-DAD Method for the Determination of Seven Antioxidants in a Nano-Emulsion: Formulation and Stability Study
Authors: Kamaris Georgios , Dalavitsou Antonia , Markopoulou Catherine K.
Published: January 2024

Oxidative stress degrades skin collagen and elastin and causes inflammatory reactions that affect mitochondrial DNA leading to aging. In the present study, a potential cosmetic nano-emulsion (o/w) of seven substances (chlorogenic acid, caffeine, rutin, hesperidin, quercetin, α-tocopherol and retinol) with antioxidant and anti-aging properties was prepared and analyzed. The lipophilic components were entrapped in the dispersed nanoparticles (jojoba) of the emulsion while the hydrophilic ones dissolved in the aqueous phase (glycerol/water). Suitable excipients were selected using an experimental design methodology with two mixtures and two responses (particle size and zeta potential). The quantitative extraction of chlorogenic acid and caffeine from Crithmum maritimum L. plant and coffee beans (Coffea arabica L.) and their stability were also studied. The analysis of the substances was carried out on an HPLC-DAD, with a phenyl column and gradient elution system (solvent A: water with 0.2% formic acid and B: acetonitrile with 0.2% formic acid). Validation of the method was performed in terms of linearity (r2 > 0.998), precision and repeatability (%RSD < 2) while the limits of detection (LLODs) and quantification (LLOQs) were also determined. The antioxidants were quantified after being extracted from the substrate (%recovery 96.7–102.5, %RSD < 2).
Effect of Outdoor Leisure Participants on Leisure Identity, Leisure Flow, Leisure Satisfaction, and Re-Participation Intention
Authors: Ahn Byoung-Wook , Song Won-Ick
Published: January 2024

The aim of this study was to study the effect of leisure identity, flow, satisfaction, and re-participation intention among outdoor leisure participants in South Korea. Due to the recent COVID-19 pandemic, there has been a tendency to more frequently participate in outdoor leisure as opposed to indoor leisure. Leisure activities bestow various benefits. Therefore, this study was used to analyze the relationships amongst leisure identity, leisure flow, leisure satisfaction, and re-participation intention among various theories about leisure. The participants in this study were collected from 369 people who were frequent participants in outdoor leisure. For the data analysis, the researchers used frequency, confirmatory analysis, reliability, correlation, and SEM. The findings were as follows: First, leisure identity had wielded an influence on leisure flow. Second, leisure identity had an influence on leisure satisfaction. Third, leisure identity had an influence on re-participation intention. Fourth, leisure flow did not have any significant influence on leisure satisfaction. Fifth, leisure flow did not have any significant influence on leisure satisfaction. And finally, leisure satisfaction had an influence on re-participation intention. In the era of the COVID-19 pandemic, a new leisure identity has been formed, and it is believed to be a new study on leisure flow, leisure satisfaction, and re-participation intention. This study aims to provide basic data for constructing infrastructure to enable continued participation in outdoor leisure in Korea.
Two-Stage Stochastic Scheduling of Cascaded Hydropower–Wind–Photovoltaic Hybrid Systems Considering Contract Decomposition and Spot Market
Authors: Li Yang , Fang Ni , He Shengming , Wu Feng , Li Outing , Shi Linjun , Ding Renshan
Published: January 2024

With the advancement of China’s electricity markets and the continuous development of renewable energy sources (RESs), it is of great importance to investigate the strategic behavior of RESs in electricity markets. In this paper, a two-stage stochastic optimization model is proposed for a hybrid energy system composed of cascade hydropower plants, wind farms, and photovoltaic stations. Firstly, typical scenarios are generated based on Latin hypercube sampling (LHS) and the K-means clustering algorithm to represent uncertainties of wind–photovoltaic power outputs. Then, with an analysis of China’s electricity market structure, a two-stage coordinated scheduling model of hydropower–wind–photovoltaic hybrid systems in electricity markets is established with the objective of maximizing total revenues considering bilateral contract decomposition, the day-ahead energy market, and the real-time balance market. In addition, the proposed model is transformed into a mixed-integer linear programming (MILP) problem for computational convenience. As shown in an analysis of case studies, cascade hydropower plants can compensate for the fluctuation in wind and photovoltaic power outputs to reduce financial risks caused by uncertainties of wind and photovoltaic power generation. Simulation results show that compared with uncoordinated operation, the coordinated operation of hydropower–wind–photovoltaic hybrid systems increases total revenue by 1.08% and reduces the imbalance penalty by 29.85%.