bluuebunny/crossref_metadata_2025 huggingface dataset:
Mechanisms Governing Anaphylaxis: Inflammatory Cells, Mediators, Endothelial Gap Junctions and Beyond
Authors: Nguyen Samantha Minh Thy , Rupprecht Chase Preston , Haque Aaisha , Pattanaik Debendra , Yusin Joseph , Krishnaswamy Guha
Published: July 2021

Anaphylaxis is a severe, acute, life-threatening multisystem allergic reaction resulting from the release of a plethora of mediators from mast cells culminating in serious respiratory, cardiovascular and mucocutaneous manifestations that can be fatal. Medications, foods, latex, exercise, hormones (progesterone), and clonal mast cell disorders may be responsible. More recently, novel syndromes such as delayed reactions to red meat and hereditary alpha tryptasemia have been described. Anaphylaxis manifests as sudden onset urticaria, pruritus, flushing, erythema, angioedema (lips, tongue, airways, periphery), myocardial dysfunction (hypovolemia, distributive or mixed shock and arrhythmias), rhinitis, wheezing and stridor. Vomiting, diarrhea, scrotal edema, uterine cramps, vaginal bleeding, urinary incontinence, dizziness, seizures, confusion, and syncope may occur. The traditional (or classical) pathway is mediated via T cells, Th2 cytokines (such as IL-4 and 5), B cell production of IgE and subsequent crosslinking of the high affinity IgE receptor (FcεRI) on mast cells and basophils by IgE-antigen complexes, culminating in mast cell and basophil degranulation. Degranulation results in the release of preformed mediators (histamine, heparin, tryptase, chymase, carboxypeptidase, cathepsin G and tumor necrosis factor alpha (TNF-α), and of de novo synthesized ones such as lipid mediators (cysteinyl leukotrienes), platelet activating factor (PAF), cytokines and growth factors such as vascular endothelial growth factor (VEGF). Of these, histamine, tryptase, cathepsin G, TNF-α, LTC4, PAF and VEGF can increase vascular permeability. Recent data suggest that mast cell-derived histamine and PAF can activate nitric oxide production from endothelium and set into motion a signaling cascade that leads to dilatation of blood vessels and dysfunction of the endothelial barrier. The latter, characterized by the opening of adherens junctions, leads to increased capillary permeability and fluid extravasation. These changes contribute to airway edema, hypovolemia, and distributive shock, with potentially fatal consequences. In this review, besides mechanisms (endotypes) underlying IgE-mediated anaphylaxis, we also provide a brief overview of IgG-, complement-, contact system-, cytokine- and mast cell-mediated reactions that can result in phenotypes resembling IgE-mediated anaphylaxis. Such classifications can lead the way to precision medicine approaches to the management of this complex disease.
P62 Links the Autophagy Pathway and the Ubiquitin–Proteasome System in Endothelial Cells during Atherosclerosis
Authors: Kim SeJeong , Lee WoongJin , Cho KyoungJoo
Published: July 2021

Among autophagy-related molecules, p62/SQSTM1 is an adaptor for identifying and delivering intracellular cargo for degradation. Since ubiquitination is reversible, it has a switch role in autophagy. Ubiquitination is also involved in regulating autophagy in a timely manner. This study aimed to elucidate how p62-mediated autophagy is regulated in human endothelial cells and macrophages under atherosclerotic conditions, focusing on the lysosomal and proteasomal pathways. Co-cultured HUVECs and THP-1 cells were exposed to oxLDL (50 μg/mL) and autophagy was assessed. To downregulate p62, siRNA was administered, and the E3 ligases were inhibited by Heclin or MLN4924 treatment under the condition that cellular inflammatory processes were stimulated by oxLDL simultaneously initiated autophagy. Downregulating p62 induced an alternative degradation system, and the E3 ligases were found to be involved in the progression of atherosclerosis. Collectively, the present study demonstrated that the endothelial lipid accumulation under atherosclerotic conditions was caused by lysosomal dysfunction associated with autophagy.
Modulation of Rice Leaf Angle and Grain Size by Expressing OsBCL1 and OsBCL2 under the Control of OsBUL1 Promoter
Authors: Jang Seonghoe , Cho Jwa-Yeong , Do Gyung-Ran , Kang Yeeun , Li Hsing-Yi , Song Jaeeun , Kim Ho-Youn , Kim Beom-Gi , Hsing Yue-Ie
Published: July 2021

Leaf angle and grain size are important agronomic traits affecting rice productivity directly and/or indirectly through modulating crop architecture. OsBC1, as a typical bHLH transcription factor, is one of the components comprising a complex formed with LO9-177 and OsBUL1 contributing to modulation of rice leaf inclination and grain size. In the current study, two homologues of OsBC1, OsBCL1 and OsBCL2 were functionally characterized by expressing them under the control of OsBUL1 promoter, which is preferentially expressed in the lamina joint and the spikelet of rice. Increased leaf angle and grain length with elongated cells in the lamina joint and the grain hull were observed in transgenic rice containing much greater gibberellin A3 (GA3) levels than WT, demonstrating that both OsBCL1 and OsBCL2 are positive regulators of cell elongation at least partially through increased GA biosynthesis. Moreover, the cell elongation was likely due to cell expansion rather than cell division based on the related gene expression and, the cell elongation-promoting activities of OsBCL1 and OsBCL2 were functional in a dicot species, Arabidopsis.
Differential Serotonin Uptake Mechanisms at the Human Maternal–Fetal Interface
Authors: Baković Petra , Kesić Maja , Perić Maja , Bečeheli Ivona , Horvatiček Marina , George Meekha , Čičin-Šain Lipa , Desoye Gernot , Wadsack Christian , Panzenboeck Ute , Štefulj Jasminka
Published: July 2021

Serotonin (5-HT) plays an extensive role during pregnancy in regulating both the placental physiology and embryonic/fetal development. The uptake of 5-HT into cells is central to the control of local concentrations of 5-HT near its molecular targets. Here, we investigated the mechanisms of 5-HT uptake into human primary placental cells and cord blood platelets, all isolated immediately after birth. Trophoblasts and cord blood platelets showed 5-HT uptake with similar Michaelis constant (Km) values (~0.6 μM), typical of the high-affinity serotonin transporter (SERT). The uptake of 5-HT into trophoblasts was efficiently inhibited by various SERT-targeting drugs. In contrast, the uptake of 5-HT into feto-placental endothelial cells was not inhibited by a SERT blocker and showed a Km value (~782 μM) in the low-affinity range. Consistent with this, SERT mRNAs were abundant in term trophoblasts but sparse in feto-placental endothelial cells, whereas the opposite was found for the low-affinity plasma membrane monoamine transporter (PMAT) mRNAs. Organic cation transporter (OCT) 1, 2, and 3 mRNAs were absent or sparse in both cell types. In summary, the results demonstrate, for the first time, the presence of functional 5-HT uptake systems in feto-placental endothelial cells and fetal platelets, cells that are in direct contact with fetal blood plasma. The data also highlight the sensitivity to various psychotropic drugs of 5-HT transport into trophoblasts facing the maternal blood. The multiple, high-, and low-affinity systems present for the cellular uptake of 5-HT underscore the importance of 5-HT homeostasis at the maternal–fetal interface.
Talent Competitiveness Evaluation of the Chongqing Intelligent Industry Based on Using the Entropy TOPSIS Method
Authors: Xu Xianhang , Arshad Mohd Anuar , Mahmood Arshad
Published: July 2021

Talent is the foundation of industrial development, based on the movement process and the role of the talent competitiveness cycle. This study uses the Entropy TOPSIS method to evaluate the talent competitiveness of Chongqing in comparison to Beijing, Tianjin, Shanghai, Jiangsu, Zhejiang, Guangdong, Sichuan, and Shaanxi from five dimensions of human resources, talent contribution, talent investment, development support, and development environment. The research shows that the talent competitiveness of the intelligent industry in Chongqing ranks eighth as compared to other eight provinces and cities. The result shows that there is a big gap in human resources, talent contribution and talent investment compared with the developed areas, while development support and environment level were found to be relatively backward. It is suggested to strengthen the policy implementation from the aspects of high-end talent introduction, investment in higher education, industrial ecological system and talent development environment.
Identification and Expression Profile of Chemosensory Genes in the Small Hive Beetle Aethina tumida
Authors: Wu Lixian , Zhai Xin , Li Liangbin , Li Qiang , Liu Fang , Zhao Hongxia
Published: July 2021

Aethina tumida is a parasite and predator of honeybee causing severe loss to the bee industry. No effective and environmentally friendly methods are available to control this pest at present. Chemosensory genes play key roles in insect behavior which can potentially be used as targets for developing environmentally friendly pest control agents. In this study, the putative chemosensory genes in antennae and forelegs of A. tumida involved in olfaction or contact chemical communication of adults were investigated using RNA transcriptome sequencing and PCR methods. Based on transcriptomic data, unigenes encoding 38 odorant receptors (ORs), 24 ionotropic receptors (IRs), 14 gustatory receptors (GRs), 3 sensory neuron membrane proteins (SNMPs), 29 odorant binding proteins (OBPs), and 22 chemosensory proteins (CSPs) were identified. The analyses of tissue expression profiles revealed that genes encoding 38 ORs, 13 antennal IRs, 11 GRs, 1 SNMP, 24 OBPs, and 12 CSPs were predominately expressed in antennae. No significant differences in expression levels of these genes were found between males and females. Genes encoding 5 non-NMDA iGluRs, 3 GRs, 2 SNMPs, 5 OBPs, and 12 CSPs were predominately expressed in forelegs. RT-PCR assays for SNMPs, OBPs and CSPs further revealed that 3 OBPs (AtumOBP3, 26 and 28) and 3 CSPs (AtumCSP7, 8 and 21) were highly expressed in antennae. Our results enrich the gene inventory of A. tumida and facilitate the discovery of potential novel targets for developing new pest control measures.
An Assistive Role of a Machine Learning Network in Diagnosis of Middle Ear Diseases
Authors: Byun Hayoung , Yu Sangjoon , Oh Jaehoon , Bae Junwon , Yoon Myeong Seong , Lee Seung Hwan , Chung Jae Ho , Kim Tae Hyun
Published: July 2021

The present study aimed to develop a machine learning network to diagnose middle ear diseases with tympanic membrane images and to identify its assistive role in the diagnostic process. The medical records of subjects who underwent ear endoscopy tests were reviewed. From these records, 2272 diagnostic tympanic membranes images were appropriately labeled as normal, otitis media with effusion (OME), chronic otitis media (COM), or cholesteatoma and were used for training. We developed the “ResNet18 + Shuffle” network and validated the model performance. Seventy-one representative cases were selected to test the final accuracy of the network and resident physicians. We asked 10 resident physicians to make diagnoses from tympanic membrane images with and without the help of the machine learning network, and the change of the diagnostic performance of resident physicians with the aid of the answers from the machine learning network was assessed. The devised network showed a highest accuracy of 97.18%. A five-fold validation showed that the network successfully diagnosed ear diseases with an accuracy greater than 93%. All resident physicians were able to diagnose middle ear diseases more accurately with the help of the machine learning network. The increase in diagnostic accuracy was up to 18% (1.4% to 18.4%). The machine learning network successfully classified middle ear diseases and was assistive to clinicians in the interpretation of tympanic membrane images.
The Impact of Long-Term Antibiotic Therapy of Cutaneous Adverse Reactions to EGFR Inhibitors in Colorectal Cancer Patients
Authors: Mihai Mara Mădălina , Ion Ana , Giurcăneanu Călin , Nițipir Cornelia , Popa Ana-Maria , Chifiriuc Mariana-Carmen , Popa Mircea Ioan , Říčař Jan , Popa Liliana Gabriela , Sârbu Ionela , Lazăr Veronica
Published: July 2021

Colorectal cancer (CRC) is an important public health issue, in terms of incidence and mortality, with approximately 1.8 million new cases reported worldwide in 2018. Advancements in understanding pathophysiological key steps in CRC tumorigenesis have led to the development of new targeted therapies such as those based on epidermal growth factor receptor inhibitors (EGFR inhibitors). The cutaneous adverse reactions induced by EGFR inhibitors, particularly papulopustular rash, often require long-term antibiotic treatment with tetracycline agents (mostly minocycline and doxycycline). However, this raises several issues of concern: possible occurrence of gut dysbiosis in already vulnerable CRC patients, selection of highly antibiotic resistant and/or virulent clones, development of adverse reactions related to tetracyclines, interference of antibiotics with the response to oncologic therapy, with a negative impact on disease prognosis etc. In the context of scarce information regarding these issues and controversial opinions regarding the role of tetracyclines in patients under EGFR inhibitors, our aim was to perform a thorough literature review and discuss the main challenges raised by long-term use of tetracyclines in advanced CRC patients receiving this targeted therapy.
Tracking Fungal Growth: Establishment of Arp1 as a Marker for Polarity Establishment and Active Hyphal Growth in Filamentous Ascomycetes
Authors: Groth Anika , Schunke Carolin , Reschka Eva , Pöggeler Stefanie , Nordzieke Daniela
Published: July 2021

Polar growth is a key characteristic of all filamentous fungi. It allows these eukaryotes to not only effectively explore organic matter but also interact within its own colony, mating partners, and hosts. Therefore, a detailed understanding of the dynamics in polar growth establishment and maintenance is crucial for several fields of fungal research. We developed a new marker protein, the actin-related protein 1 (Arp1) fused to red and green fluorescent proteins, which allows for the tracking of polar axis establishment and active hyphal growth in microscopy approaches. To exclude a probable redundancy with known polarity markers, we compared the localizations of the Spitzenkörper (SPK) and Arp1 using an FM4-64 staining approach. As we show in applications with the coprophilous fungus Sordaria macrospora and the hemibiotrophic plant pathogen Colletotrichum graminicola, the monitoring of Arp1 can be used for detailed studies of hyphal growth dynamics and ascospore germination, the interpretation of chemotropic growth processes, and the tracking of elongating penetration pegs into plant material. Since the Arp1 marker showed the same dynamics in both fungi tested, we believe this marker can be broadly applied in fungal research to study the manifold polar growth processes determining fungal life.
Needs, Preferences, and Values during Different Treatment Decisions of Patients with Differentiated Thyroid Cancer
Authors: Koot Anna , Netea-Maier Romana , Ottevanger Petronella , Hermens Rosella , Stalmeier Peep
Published: July 2021

Background: The purpose of this study was to identify the needs, preferences, and values of patients with differentiated thyroid cancer (DTC) and the physicians treating patients with DTC regarding two different treatment decisions, namely: the extent of primary surgery (low-risk patients) and the tyrosine kinase inhibitor (TKI) treatment (high-risk patients). Methods: A qualitative study was conducted. There were two physician focus groups discussing the extent of primary surgery. One included endocrinologists (n = 4) and surgeons (n = 5), and the other included nuclear medicine physicians (n = 3) treating patients with low-risk DTC. The physicians focus group discussing waiting or starting TKIs included endocrinologists (n = 2) and oncologists (n = 5) treating patients with advanced radioactive iodide (RAI) refractory DTC. Moreover, one patient focus group per treatment decision took place. In total 13 patients and 19 physicians participated. Interviews were audio-taped, fully transcribed verbatim, and analyzed. Results: Several themes were identified. Patients, but not physicians, mentioned the importance of a strong doctor–patient relationship. Patients in both treatment decision groups wanted to receive more detailed information, whereas physicians preferred providing more general information. Patients in the TKI decision group focused on palliative care, whereas physicians focused more on the effect and benefit of TKIs. Conclusions: Considering the identified themes in DTC, based on the patients’ needs, preferences, and values, enables us to improve doctor–patient communication and to develop decision support tools.
Structure, Activity and Function of the NSD3 Protein Lysine Methyltransferase
Authors: Rathert Philipp
Published: July 2021

NSD3 is one of six H3K36-specific lysine methyltransferases in metazoans, and the methylation of H3K36 is associated with active transcription. NSD3 is a member of the nuclear receptor-binding SET domain (NSD) family of histone methyltransferases together with NSD1 and NSD2, which generate mono- and dimethylated lysine on histone H3. NSD3 is mutated and hyperactive in some human cancers, but the biochemical mechanisms underlying such dysregulation are barely understood. In this review, the current knowledge of NSD3 is systematically reviewed. Finally, the molecular and functional characteristics of NSD3 in different tumor types according to the current research are summarized.
Structures and Biological Activities of Diketopiperazines from Marine Organisms: A Review
Authors: Song Zhiqiang , Hou Yage , Yang Qingrong , Li Xinpeng , Wu Shaohua
Published: July 2021

Diketopiperazines are potential structures with extensive biological functions, which have attracted much attention of natural product researchers for a long time. These compounds possess a stable six-membered ring, which is an important pharmacophore. The marine organisms have especially been proven to be a wide source for discovering diketopiperazine derivatives. In recent years, more and more interesting bioactive diketopiperazines had been found from various marine habitats. This review article is focused on the new 2,5-diketopiperazines derived from marine organisms (sponges and microorganisms) reported from the secondary half-year of 2014 to the first half of the year of 2021. We will comment their chemical structures, biological activities and sources. The objective is to assess the merit of these compounds for further study in the field of drug discovery.
Simulation of Organic Liquid Products Deoxygenation by Multistage Countercurrent Absorber/Stripping Using CO2 as Solvent with Aspen-HYSYS: Thermodynamic Data Basis and EOS Modeling
Authors: Costa Elinéia Castro , de Araújo Silva Welisson , Menezes Eduardo Gama Ortiz , da Silva Marcilene Paiva , Cunha Vânia Maria Borges , de Andrade Mâncio Andréia , Santos Marcelo Costa , da Mota Sílvio Alex Pereira , Araújo Marilena Emmi , Machado Nélio Teixeira
Published: July 2021

In this work, the thermodynamic data basis and equation of state (EOS) modeling necessary to simulate the fractionation of organic liquid products (OLP), a liquid reaction product obtained by thermal catalytic cracking of palm oil at 450 °C, 1.0 atmosphere, with 10% (wt.) Na2CO3 as catalyst, in multistage countercurrent absorber/stripping columns using supercritical carbon dioxide (SC-CO2) as solvent, with Aspen-HYSYS was systematically investigated. The chemical composition of OLP was used to predict the density (ρ), boiling temperature (Tb), critical temperature (Tc), critical pressure (Pc), critical volume (Vc), and acentric factor (ω) of all the compounds present in OLP by applying the group contribution methods of Marrero-Gani, Han-Peng, Marrero-Pardillo, Constantinou-Gani, Joback and Reid, and Vetere. The RK-Aspen EOS used as thermodynamic fluid package, applied to correlate the experimental phase equilibrium data of binary systems OLP-i/CO2 available in the literature. The group contribution methods selected based on the lowest relative average deviation by computing Tb, Tc, Pc, Vc, and ω. For n-alkanes, the method of Marrero-Gani selected for the prediction of Tc, Pc and Vc, and that of Han-Peng for ω. For alkenes, the method of Marrero-Gani selected for the prediction of Tb and Tc, Marrero-Pardillo for Pc and Vc, and Han-Peng for ω. For unsubstituted cyclic hydrocarbons, the method of Constantinou-Gani selected for the prediction of Tb, Marrero-Gani for Tc, Joback for Pc and Vc, and the undirected method of Vetere for ω. For substituted cyclic hydrocarbons, the method of Constantinou-Gani selected for the prediction of Tb and Pc, Marrero-Gani for Tc and Vc, and the undirected method of Vetere for ω. For aromatic hydrocarbon, the method of Joback selected for the prediction of Tb, Constantinou-Gani for Tc and Vc, Marrero-Gani for Pc, and the undirected method of Vetere for ω. The regressions show that RK-Aspen EOS was able to describe the experimental phase equilibrium data for all the binary pairs undecane-CO2, tetradecane-CO2, pentadecane-CO2, hexadecane-CO2, octadecane-CO2, palmitic acid-CO2, and oleic acid-CO2, showing average absolute deviation for the liquid phase (AADx) between 0.8% and 1.25% and average absolute deviation for the gaseous phase (AADy) between 0.01% to 0.66%.
Recent Advances in the Clinical Translation of Silicon Fluoride Acceptor (SiFA) 18F-Radiopharmaceuticals
Authors: Gower-Fry Lexi , Kronemann Travis , Dorian Andreas , Pu Yinglan , Jaworski Carolin , Wängler Carmen , Bartenstein Peter , Beyer Leonie , Lindner Simon , Jurkschat Klaus , Wängler Björn , Bailey Justin J. , Schirrmacher Ralf
Published: July 2021

The incorporation of silicon fluoride acceptor (SiFA) moieties into a variety of molecules, such as peptides, proteins and biologically relevant small molecules, has improved the generation of 18F-radiopharmaceuticals for medical imaging. The efficient isotopic exchange radiofluorination process, in combination with the enhanced [18F]SiFA in vivo stability, make it a suitable strategy for fluorine-18 incorporation. This review will highlight the clinical applicability of [18F]SiFA-labeled compounds and discuss the significant radiotracers currently in clinical use.
Predictors of postoperative urinary retention after semiclosed hemorrhoidectomy
Authors: Jeong Hong Yoon , Song Seok Gyu , Lee Jong Kyun
Published: July 2021

Purpose: This study was performed to analyze the predictors that might contribute to urinary retention following semiclosed hemorrhoidectomy under spinal anesthesia.Methods: This retrospective study enrolled 2,176 consecutive patients with symptomatic grade III to IV hemorrhoids who underwent semiclosed hemorrhoidectomy between September 2018 and September 2019.Results: Among the 2,176 patients, 1,878 (86.3%) had no postoperative urinary retention, whereas 298 (13.7%) developed urinary retention after hemorrhoidectomy. The percentage of males was significantly higher in the retention group than in the non-retention group (60.4% vs. 48.1%; P=0.001). The risk of urinary retention was 1.52-fold higher in males than in females (95% confidence interval [CI], 1.13–2.04; P=0.005), 1.62-fold higher in old age (95% CI, 1.14–2.28; P=0.006), and 1.37-fold higher with high body mass index (BMI) (95% CI, 1.04–1.81; P=0.025). Patients with ≥4 resected hemorrhoids had a higher odds ratio (OR) of 1.46 (95% CI, 1.12–1.89; P=0.005) than patients with <4 resected hemorrhoids. Among the supplementary medication, patients who used analgesics had a higher OR of 2.06 (95% CI, 1.57–2.68; P=0.001) than those who did not.Conclusion: Male sex, age, high BMI, number of resected hemorrhoids, and supplementary analgesics are independent risk factors for urinary retention after semiclosed hemorrhoidectomy.
DEVELOPMENT OF AN INTELLIGENT SYSTEM FOR MANAGING EDGE COMPUTING
Authors: ХАКИМОВ А.А. , МУТХАННА А.С. , ВЫБОРНОВА А.И.
Published: July 2021

Рассмотрена возможность размещения интернет-сервисов как на удаленных облаках, так и на распределенных граничных вычислительных системах. Предложена динамическая топология сети и размещения сервисов в граничных вычислительных системах с использованием генетического алгоритма для анализа и прогнозирования трафика сервисов. Представлены эффективные методы прогнозирования и предиктивной миграции сервисов для граничных вычислительных систем. Проанализированы проблемы построения высоконагруженных сетей и метода их решения путем выгрузки сервисов на граничные периферийные устройства. Предложена модель взаимодействий оператора сети и сторонних сервис-провайдеров для оптимальной выгрузки услуг. Показано, что эффективность сетевого оборудования может быть увеличена более чем на 30%.

The possibility of hosting Internet services both in remote clouds and in distributed edge computing systems is considered. Dynamic network topology and placement of services in edge computing systems using a genetic algorithm to analyze and predict service traffic are proposed. Effective methods for forecasting and predictive migration of services for edge computing systems are presented. An analysis of the problems of constructing high-load networks and the method of their solution by offloading services to edge peripheral devices are given. A model of interactions between the network operator and third-party service providers for optimal offloading of services is proposed. It is shown that the efficiency of network equipment can be increased by more than 30%.
Short communication: Canarium odontophyllum Miq. (dabai) stem bark arrested HCT 116 cell line at G0/G1 checkpoint
Authors: Basri Dayang Fredalina , Ee Ngieng Sheng , Rajab Nor Fadilah , Yoganathan Dharrshine , Ahmad Raus Raha
Published: July 2021

Canarium odontophyllum Miq. is an exotic plant which is native in Borneo and belong to the Burseraceae family. It contains phytochemicals such as saponin, terpenoid, flavonoid and phenolic compound with potential anticancer property. It has been found that the extract of this plant negatively affected colorectal cancer cells by stimulating apoptosis. To elucidate the apoptosis mechanism induced by the plant extract, this study evaluated the effect of C. odontophyllum stem bark acetone extract on cell cycle distribution of HCT 116 cell line using propidium iodide assay. For this purpose, IC50 of the acetone extract of C. odontophyllum was first determined by treating HCT 116 cells with the extract for 24, 48 and 72 hours. It was found that the acetone extract of C. odontophyllum inhibited proliferation of HCT 116 at IC50 value of 55.09 ± 18.29 µg/mL for 24 hours treatment, 37.81 ± 5.09 µg/mL for 48 hours treatment, and 114.9 ± 16.08 µg/mL following 72 hours treatment. Using IC50 value of 48 hours treatment, it was observed that C. odontophyllum acetone extract arrested the HCT 116 cells at G0/G1 checkpoint. Based on this result, it can be concluded that one of the apoptosis mechanisms induced by C. odontophyllum is by arresting cell cycle of HCT 116 cells at G0/G1 checkpoint. This finding warrants further investigation on how C. odontophyllum causes the cell cycle arrest and its potential to become anticancer agent.
Application of Grey Wolf Optimization Algorithm: Recent Trends, Issues, and Possible Horizons
Authors: DADA Emmanuel , JOSEPH Stephen , OYEWOLA David , FADELE Alaba Ayotunde , CHİROMA Haruna , ABDULHAMİD Shafi'i Muhammad
Published: July 2021

The development of Grey Wolf Optimisation (GWO) Algorithm was motivated by the biological behaviours of swarm of wolves hunting for prey. This paper presents recent progress on Grey Wolf Optimization (GWO) algorithm, its variants and their applications, issues, and likely prospects. The review revealed that opportunities still exists for development of more robust and stable variants of GWO that will overcome the shortcomings of existing variants. This review has the potential to stimulate researchers in the area of nature-inspired algorithms to further advance the effectiveness of the GWO and its ability to solve problems. Such problems can be real-life, complicated and nonlinear optimization problems in different domain of human endeavour. Suggestions for new research directions that have the capacity to increase the performance of GWO are presented. It is expected that this paper will serve as reading material for beginners whereas experienced researchers can also use it as an article yardstick for further development of GWO algorithms.
Design and Fabrication of Compact Microstrip Low-pass Filter with Thin Film Technique for Wideband Applications
Authors: TÜZÜN Reşat , AKÇAM Nursel , OKAN Tayfun
Published: July 2021

This paper introduces a compact microstrip low-pass filter (LPF) with a very sharp rejection. The proposed LPF is designed to be used for electronic warfare in defense industry. It has 62 dB/GHz attenuation rate, 10 GHz of stopband width and 48.6 dB of stopband attenuation. Moreover, it also has a wide passband and has a high cut-off frequency (f_c) of 6 GHz. The designed filter has been fabricated using alumina (Al2O3) substrate and titanium-gold (Ti-Au) coating on top of that with thin film technique in a clean room. The experimental measurements of the proposed LPF show great agreement with the simulation results, which validates that the presented filter is convenient to be used for applications up to C-band in electronic warfare systems.
An Effective Image Encryption Algorithm Using Bit Reversal Permutation and a New Chaotic Map
Authors: OĞRAŞ Hidayet , TÜR Mehmet Rıda
Published: July 2021

In this paper, a different approach to create a new chaotic model and an effective image encryption structure using Bit reversal permutation are proposed. Compared to most frequently used and well known chaotic maps, such as Logistic map, Sine map or Tent map, a new chaotic system based on Logistic map with Sine map is designed and used as an encryption key generator in the proposed algorithm. The new map has increased initial value sensitivity according to the results of Lyapunov analysis and shown better randomness output according to the chaotic trajectory analysis. In cryptography, a good key should be a stochastic and supposed to be sufficiently random and uniformly distributed with equal probability for an effective encryption. The designed chaotic map provides these properties very well. Before the basic encryption process, the Bit reversal method makes all pixel positions of input image rearranged in order to reduce the strong relation of adjacent pixels for higher encryption strength, which will enable strategic information sharing for production planning when this method analyzes a wind energy map in power plants. Other experimental results confirm that the proposed image encryption scheme has sufficient security, an effective encryption capability and can be transferred between power systems, keeping energy planning secret strategically.
Healing, Community, and Humanity: How Students and Teachers Want to Reinvent Schools Post-COVID
Authors: Reich Justin , Mehta Jal
Published: July 2021

Understanding the experiences of students and teachers during pandemic schooling is vital to educational recovery and building back better. In the spring of 2021 as the school year was coming to close, we conducted three research exercises: 1) we invited 200 teachers to interview their students about the past year and share their findings, 2) we interviewed 50 classroom teachers, and 3) we conducted ten multistakeholder design charrettes with students, teachers, school leaders, and family members to begin planning for the 2021-2022 recovery year. Rather than a "return to normal" or the targeting of a narrowly-conceived "learning loss," the students and educators in our study emphasized themes of healing, community, and humanity as key learnings from the pandemic year and essential values to rebuilding schools. We recommend that in the 2021-2022 year, schools create structures for community members to reflect on the pandemic year, celebrate resilience, grieve what has been lost, and imagine how the lessons learned from a tumultuous year can inform more equitable, resilient school systems for the future. We provide guidance on four reflection protocols to use in school communities to advance this work.
Shari`ah, Brain Death, and Organ Transplantation
Authors: Grundmann Johannes
Published: July 2021

Introduction

In the early 1980s, comprehensive developments in the public health system as well as markedly improved possibilities for organ transplantation due to the introduction of immune suppressants,2 namely, the artificial prevention of organ rejection, led to a sudden expansion of transplantation medicine in the Near and Middle East. Long-term artificial respiration, along with improved and expanded intensive care units, enabled respiration and circulation to be maintained despite the partial or complete loss of brain function. This, in turn, secured the necessary blood supply to the organs until they could be removed. Against this backdrop, a comprehensive process of discussion on the factual connections between postmortem organ transplantation and the criterion for determining brain death developed.
Efek Senyawa Timbal Asetat Terhadap Pertumbuhan Dan Kandungan Pigmen Klorofil Mikroalga Dunaliella sp.
Authors: Tamalonggehe Joshep , Kemer Kurniati , Paransa Darus Sa’adah J , Mantiri Desy MH , Kawung Nickson J , Undap Suzanne L
Published: July 2021

Microalgae is one of the marine biota that has an important role in the waters because it acts as a supplier of food in the waters. Microalgae is a biological source that needs to be exploited because it is rich in essential compounds. Dunaliella sp. is one of the many micro algae used as research. Utilization of Dunaliella sp. quite diverse and has been marketed in developed countries because of its very attractive economic value. This study aims to determine the effects of lead acetate compounds on growth and content of chlorophyll pigments microalgae Dunaliella sp. The results obtained in this study are the lead acetate compounds can affect the number of cells in the growth of microalgae and analysis results obtained with a spectrophotometer showed that the extraction concentration of control day 5 (Exponential Phase) was higher than the concentration of 15 ppm and 25 ppm, whereas extraction on day 21 (Death Phase) concentration of 15 ppm was higher than 25 ppm.Keywords : Dunalella sp., Lead Acetate, Pigment chlorophyll RingkasanMikroalga adalah salah satu biota laut yang memiliki peran penting di perairan karena berfungsi sebagai pemasok makanan di perairan. Mikroalga adalah sumber biologis yang perlu dieksploitasi karena kaya akan senyawa esensial. Dunaliella sp. adalah salah satu dari banyak mikroalga yang digunakan sebagai penelitian. Pemanfaatan Dunaliella sp. cukup beragam dan telah dipasarkan di negara maju karena nilai ekonominya yang sangat menarik. Penelitian ini bertujuan untuk mengetahui efek senyawa timbal asetat terhadap pertumbuhan dan kandungan pigmen klorofil Dunaliella sp. Hasil yang diperoleh dalam penelitian ini adalah senyawa timbal asetat dapat mempengaruhi jumlah sel dalam pertumbuhan mikroalga, penurunan jumlah sel mengikuti konsentrasi timbal asetat yang diberikan dan hasil analisis yang diperoleh dengan spektrofotometer menunjukkan bahwa konsentrasi ekstraksi kontrol hari 5 (Fase Eksponensial) lebih tinggi daripada konsentrasi 15 ppm dan 25 ppm, sedangkan ekstraksi hari 21 (Fase Kematian) konsentrasi 15 ppm lebih tinggi dari 25 ppm.Kata kunci : Dunaliella sp., Timbal Asetat, Pigmen Klorofil
AKTIVITAS SENYAWA ANTIBAKTERI DAN ANTI-UV DARI Phyllidia varicosa (Cuvier, 1804) DAN BAKTERI SIMBIONNYA (NUDIBRANCHIA GASTROPODA) DARI PERAIRAN TANJUNG MANDOLANG, MINAHASA
Authors: Ukar Melania , Bara Robert A , Rumengan Inneke F M , Losung Fitje , Salaki Meiske , Warouw Veibe
Published: July 2021

Phyllidia varicosa is an organism belongs to the order Nudibranchia, which is commonly known as a sea rabbit. This organism is able to synthesize secondary metabolites from food. The purpose of this study was to obtain P. varicosa extract and symbiotic bacterial extract from P. varicosa, then determine the antibacterial activity of P. varicosa extract and the symbionic bacteria extract against Escherichia coli DSM498, Bacillus megaterium DSM32T and anti-UV activity. P. varicose symbiotic bacterial were isolated and extracted. The results obtained 5 bacterial isolates. The results of antibacterial assay of isolates PhVa 1.1, PhVa 1.3, PhVa 2.1, PhVa 2.3 and PhVa 2.4 shown that these isolates have an antibacterial activity against E. coli DSM498 and B. megaterium DSM32T. Anti-UV assay results shown an absorption at UV-A with the highest value of 1.991 at λ 340 nm. Keywords: Nudibranchia, Phyllidia varicosa, Antibacterial, Anti-UV, Escherichia coli, Bacillus megaterium Abstrak Phyllidia varicosa merupakan organisme yang termasuk dalam ordo Nudibranchia, yang umumnya dikenal sebagai kelinci laut. Organisme ini mampu mensintesis metabolit sekunder dari bahan makanannya. Tujuan dari penelitian ini yaitu untuk mendapatkan ekstrak P. varicosa dan ekstrak bakteri simbion dari P. varicosa, kemudian mengamati aktivitas antibakteri dari ekstrak P. varicosa dan ekstrak bakteri simbionnya terhadap bakteri Escherichia coli DSM498 dan Bacillus megaterium DSM32T serta menguji aktivitas anti-UV. Bakteri yang bersimbion dengan P. varicosa diisolasi dan diekstraksi, lalu diuji bioaktivitas antibakteri dan diuji anti-UV terhadap ekstrak P. varicosa dan ekstrak bakteri simbionnya. Hasil akhir dari penelitian ini yaitu didapatkan 5 isolat bakteri. Hasil uji aktivitas antibakteri yaitu isolat PhVa 1.1, PhVa 1.3, PhVa 2.1, PhVa 2.3 dan PhVa 2.4 memiliki aktivitas antibakteri terhadap bakteri E. coli DSM498 dan B. megaterium DSM32T. Hasil uji anti-UV menunjukkan serapan pada UV-A dengan nilai tertinggi 1,991 pada λ 340 nm. Kata kunci: Nudibranchia, Phyllidia varicosa, Antibakteri, Anti-UV, Escherichia coli, Bacillus megaterium
KARBON PADA PADANG LAMUN DI PERAIRAN PULAU MANADO TUA
Authors: Maramis Mikhael A , Wagey Billy , Rumengan Antonius P , Sondak Calvyn F A , Opa Esry T , Kondoy Khristin F I
Published: July 2021

Peningkatan emisi gas rumah kaca (GRK) antara lain karbondioksida (CO2)  yang terjadi terus menerus mengakibatkan terjadinya pemanasan global dan perubahan iklim yang berdampak pada  pola cuaca dan mempengaruhi kehidupan di bumi. Ekosistem padang lamun diketahui memiliki kemampun dalam menyerap karbondioksida dan menyimpannya dalam waktu yang lama. Telah dilakukan penelitian untuk mengetahui keanekaragaman jenis lamun, presentasi tutupan dan kandungan karbon pada padang lamun di perairan Pulau Manado Tua. Untuk mengetahui keanekaragaman jenis dilakukan pengamatan secara langsung pada setiap stasiun penelitian dan untuk mengetahui presentase tutupan lamun menggunakan metode line transek kuadran dengan mengadopsi Seagrass Watch dimana sampel lamun juga diperoleh dari line transek kuadran pada keseluruhan stasiun kemudian dianalisis selanjutnya menggunakan metode Loss on ignition (LOI). Didapatkan hasil penelitian pada keseluruhan stasiun indeks keanekaragaman H’ 1 kategori sedang dengan presentase penutupan dan status kondisi pada stasiun negri 36% kurang kaya/kurang sehat, stasiun pangalingan  69% kaya/sehat, stasiun papindang 51% kurang kaya/kurang sehat : kandungan karbon pada keseluruhan stasiun yang didapat sebesar 222,85gC Kata Kunci :  Keanekaragaman Lamun, Presentase Penutupan, Kandungan Karbon, Line Transek,  Kuadran,  LOI (Loss On Ignition), Pulau Manado Tua.
ANALISIS STRUKTUR KOMUNITAS MANGROVE DI KAWASAN SEKITAR PT. CONCH KABUPATEN BOLAANG MONGONDOW
Authors: Pandeirot George L , Rumengan Antonius P , Paruntu Carolus P , Darwisito Surya , Ompi Medy , Wantasen Adnan S
Published: July 2021

This research was conducted for four months of April-July 2020. The purpose of this study was to determine species density, species relative density, species frequency, species relative frequency, species cover, species relative cover, index of the importance of mangrove species in the area around PT. Conch Lolak District, Bolaang Mongondow Regency, North Sulawesi Province. This research was conducted for four months of April-July 2020. The method used was a quadratic plot of 6 stations. Data collection at each station was made with a plot size of 10 × 10 m2 to measure the diameter of a tree at breast height (DBH), which has a minimum trunk circumference of 16 cm. The results showed that there were five types of mangroves, namely R. apiculata, R. mucronata, B. gymnorhiza, S. alba, and X. granatum. The highest relative density of species at station 2, namely R. mucronate 62.96%, while the lowest value at station 3, namely R. mucronata 8.77%, the highest relative frequency value at station 4, namely R. mucronata 33.33%, whereas the lowest value was at station 5, namely B. gymnorhiza 25.00%, the highest relative closure value was at station 5, namely R. mucronata 30.91%, while the lowest was at station 2, namely R. apiculata 12.88%, and the important value index the highest was at station 3, namely R. apiculata 138.09% and the lowest value was at station 5, namely X. granatum 35.05%.Kata Kunci: Mangrove, Structure in areas around PT. Conch. Conch, Rhizopora Abstrak Penelitian ini dilakukan selama 4 bulan April-Juli 2020. Tujuan penelitian ini adalah untuk mengetahui kerapatan jenis, kerapatan relatif jenis, frekuensi jenis, frekuensi relatif jenis, penutupan jenis, penutupan relatif jenis, indeks nilai penting jenis mangrove yang ada di kawasan sekitar PT. Conch Kecamatan Lolak, Kabupaten Bolaang Mongondow, Provinsi Sulawawesi Utara. Penelitian ini dilakukan selama 4 bulan April-Juli 2020. Metode yang dipakai adalah plot kuadrat sebanyak 6 stasiun. Pengambilan data pada setiap stasiun dibuat plot dengan ukuran 10 × 10 m2 untuk mengukur diameter pohon setinggi dada (DBH) yang memiliki lingkar batang minimal 16 cm. Hasil penelitian menunjukkan bahwa ada 5 jenis mangrove yaitu R. apiculata, R. mucronata, B. gymnorhiza, S. alba dan X. granatum. Kerapatan relatif jenis tertinggi pada stasiun 2, yaitu R. mucronata 62,96%, sedangkan nilai terendah pada stasiun 3, yaitu R. mucronata 8,77%, nilai Frekuensi relatif jenis tertingi pada stasiun 4, yaitu R. mucronata 33.33%, sedangkan nilai terendah pada stasiun 5, yaitu B. gymnorhiza 25.00%, nilai Penutupan relatif jenis tertinggi pada stasiun 5, yaitu R. mucronata 30,91%, sedangkan terendah pada stasiun 2, yaitu R. apiculata 12,88%, dan Indeks nilai penting tertinggi pada stasiun 3, yaitu R. apiculata 138,09% dan nilai terendah pada stasiun 5, yaitu X. granatum 35,05%.Kata Kunci: Mangrove, kawasan sekitar PT. Conch, Rhizopora
KEKAYAAN DAN POTENSI SENYAWA BIOAKTIF MAKROALGA DI PESISIR ATEP OKI, KABUPATEN MINAHASA, SULAWESI UTARA
Authors: Winowoda Silsia Dorkas , Singkoh Marina Flora Oktavine , Siahaan Ratna
Published: July 2021

This study aims to analyze the richness and potential of macroalgae bioactive compounds on the Atep Oki Coast, District of Minahasa, North Sulawesi Province. This research was conducted in October 2019 until January 2020. Sampling used the roaming method in a predetermined location that is Atep Oki tidal/ intertidal coast. Sampling locations are spread over six coordinate points. Macroalgae found on the Atep Oki Coast assessed ten species belonging to three phyla, namely Chlorophyta (green algae), Ochrophyta (brown algae) and Rhodophyta (red algae). Chlorophyta members mean six species. Ochrophyta has members of three species and members of Rhodophyta only one species. The types of macroalgae found on the Atep Oki Coast provide bioactive compounds. Keywords : Macroalgae, Bioactive Compounds, Atep Oki Minahasa CoastAbstrak Penelitian ini bertujuan untuk menganalisis kekayaan jenis dan potensi senyawa bioaktif makroalga di Pesisir Atep Oki, Kabupaten Minahasa, Provinsi Sulawesi Utara. Penelitian ini telah dilaksanakan pada bulan Oktober 2019 sampai Januari 2020. Pengambilan sampel menggunakan metode jelajah di lokasi yang telah ditentukan yaitu pesisir pasang surut/intertidal Atep Oki. Lokasi pengambilan sampel tersebar di enam titik-titik koordinat. Makroalga yang ditemukan di Pesisir Atep Oki berjumlah sepuluh species yang tergolong ke dalam tiga filum yaitu Chlorophyta (alga hijau), Ochrophyta (alga cokelat) dan Rhodophyta (alga merah). Anggota Chlorophyta berjumlah enam species. Ochrophyta memiliki anggota tiga species dan anggota Rhodophyta hanya satu species. Jenis-jenis makroalga yang ditemukan di Pesisir Atep Oki berpotensi menghasilkan senyawa bioaktif. Kata kunci : Makroalga, Senyawa Bioaktif, Pesisir Atep Oki Minahasa
PENGARUH LOGAM BERAT TIMBAL TERHADAP PERTUMBUHAN ALGA Halimeda opuntia YANG DIKULTUR DALAM WADAH TERKONTROL
Authors: Sugiarto Kristi Aji , Mantiri Desy MH , Kawung Nickson J , Rompas Rizald M , Tilaar Sandra O , Wantasen Adnan
Published: July 2021

Heavy metal is a very dangerous pollutant waste for it cannot be destroyed (non-degradable) and over time it will accumulate in the ocean. This study aims to determine the lead content in sediment, water and algal thallus as well as to determine the algae growth that has been treated with lead. The research method used was a completely randomized design (CRD) which consisted of 3 treatments, using different treatments for each sample. The highest metal content obtained in the sediment was 5,124 ppm, in the water was 0.120 ppm, and in the algae thallus was 4,816 ppm respectively. The results showed that Halimeda opuntia algae can grow even in an environment contaminated with lead heavy metal and can become a bioremediation agent by absorbing lead heavy metal. Keywords: Halimeda opuntia, Lead Heavy Metal, Algae Culture, Bioremediation             Logam berat merupakan salah satu limbah pencemar yang sangat berbahaya karena tidak dapat dihancurkan (non degradable) dan dapat terakumulasi di perairan laut. Penelitian ini bertujuan untuk mengetahui kandungan timbal pada sedimen, air dan thallus alga yang dikultur serta mengetahui pertumbuhan alga yang telah diberi perlakuan timbal. Metode penelitian mengunakan metode Rancangan Acak Lengkap (RAL) yang terdiri dari 3 perlakuan, dengan menggunakan perlakuan yang berbeda pada tiap sampel dan kontrol. Kandungan logam tertinggi yang didapatkan pada sedimen yaitu sebesar 5,124 ppm, pada air sebesar 0,120 ppm, dan pada thallus alga sebesar 4,816 ppm. Hasil yang diperoleh yaitu alga Halimeda opuntia dapat bertumbuh walaupun dalam lingkungan yang tercemar logam berat timbal serta mampu menjadi agen bioremediasi dengan menyerap logam berat timbal. Kata kunci: Halimeda opuntia, Logam BeratTimbal, Kultur Alga, Bioremediasi