bluuebunny/crossref_metadata_2025 huggingface dataset:
Enhancing Students’ Achievement in Geometry Using Laboratory-Based Instructional Method
Authors: Ajai John Tyavbee , Titilayo Ogungbile , Jimin Nguuma
Published: November 2024

This study investigated the effect of laboratory-based instructional methods on students' achievement in geometry within the educational context of Jalingo Metropolis, Taraba State, Nigeria. Recognising the potential of hands-on, experiential learning approaches in mathematics education, particularly in geometry, this research explored the effectiveness of laboratory-based instruction in enhancing student performance. Guided by three research questions and hypotheses, the quasi-experimental research design was employed on a sample of 160 Upper Basic II students, through pre-and post-test assessments. The Geometry Achievement Test (GAT) with a reliability index of 0.91 for the GAT, determined using the K-R20 method, was used for data collection. The mean and standard deviation statistics were employed to answer the three research questions, while covariance (ANCOVA) analysis was employed to test all hypotheses at a significance level of 0.05. The study found a significant improvement in geometry achievement among students exposed to the laboratory-based method, with 56% of the achievement score variance attributed to this method. Additionally, it identifies a noteworthy difference in achievement scores between male and female students taught geometry using the laboratory-based approach, with a significant interaction effect observed between the teaching method and gender. These findings emphasise the potential of incorporating laboratory-based instructional methods in mathematics education to enhance student achievement and promote a deeper understanding of geometric concepts. Overall, the study contributes valuable insights to the discourse on innovative pedagogical practices in mathematics instruction, offering guidance for educators and policymakers aiming to improve mathematics education in similar contexts.
The Acari Hypothesis, VI: human sebum and the cutaneous microbiome in allergy and in lipid homeostasis
Authors: Retzinger Andrew C. , Retzinger Gregory S.
Published: November 2024

The Acari Hypothesis posits that acarians, i.e., mites and ticks, are causative agents of IgE-mediated conditions. This report further develops The Hypothesis, providing rationale for the childhood predilection of allergy. In short, Malassezia, a fungus native to human skin and utterly dependent on sebaceous lipids, prevents allergy by deterring acarians. Because sebum output is limited before puberty, children are more prone to allergy than are adults. Competition for sebaceous lipids by Staphylococcus aureus influences not only Malassezia number—and, consequently, allergic predisposition—but also lipid homeostasis. The latter, in turn, contributes to dyslipidemia and associated conditions, e.g., the metabolic syndrome.
Prevalence of bovine paratuberculosis in Chinese cattle populations: a meta-analysis
Authors: Huiying Zhang , Mingfeng Chu , Wei Cheng , Shuiyun Chen , Yuchen Liang , Honghai Wang , Xuelong Chen , Yanping Qi
Published: November 2024

Background

Bovine paratuberculosis is a chronic infectious disease of ruminants primarily caused by Mycobacterium avium subsp. paratuberculosis (MAP). It is essentially a chronic granulomatous enteritis characterized by intractable diarrhea, progressive lethargy, and thickening of the intestinal mucosa with the formation of crumpled pouches. Bovine paratuberculosis not only adversely affects milk production and the quality of dairy products but also poses a significant threat to the economic development of dairy farming and human food security. This systematic review and meta-analysis was conducted to assess the prevalence of MAP infection among cattle herds in mainland China

Results

A total of 62 studies with data from 102,340 cattle in 24 provinces in China were selected after matching the assessment criteria. In China, the overall estimated prevalence of MAP infection in cattle was 8%(7727/102340). Interestingly, the MAP infection rate in cattle in southern China was estimated to be 2% (6/281), which was significantly lower compared with other regions of China, and the highest infection rate was 12% (1914/16008) in eastern China. MAP infection rates were related to age, average herd size, type of use, season, detection method, and sample type. Moreover, the MAP infection rate in cattle did not correlate with the publication date of the studies.

Conclusion

The analysis identified age, average herd size, type of use, and season as significant potential risk factors associated with PTB pool positivity. In addition, the detection method and sample type can also potentially affect the incidence of detected PTB.
Viable but nonculturable state in the zoonotic pathogen Bartonella henselae induced by low-grade fever temperature and antibiotic treatment
Authors: Gou Yuze , Liu Dongxia , Xin Yuxian , Wang Ting , Li Jiaxin , Xi Yiwen , Zheng Xiaoling , Che Tuanjie , Zhang Ying , Li Tingting , Feng Jie
Published: November 2024

The zoonotic pathogen Bartonella henselae is responsible for diverse human diseases, from mild to life-threatening, but it often eludes detection in culture-based assays. This study investigates the potential of B. henselae to enter a viable but nonculturable (VBNC) state when exposed to human fever temperature or antibiotics, with this state confirmed by successful resuscitation. Viability was assessed using SYBR Green I/PI staining and propidium monoazide–quantitative polymerase chain reaction (PMA-qPCR), while culturability was determined through colony-forming unit (CFU) counting on blood agar plates. Resuscitation of VBNC cells was attempted using modified Schneider’s medium with 10% defibrillated sheep blood. In the results, B. henselae cells entered a VBNC state after 19 days of exposure to 38.8°C. Antibiotics, particularly with bactericidal activity, induced the VBNC state within 4 days treatment. Successful resuscitation confirmed the VBNC state developed via the above two strategies. Transmission electron microscopy (TEM) examination revealed intact cell structures and dense cytosol in VBNC cells, with a significant increase in plasmolytic cells. Notably, VBNC cells demonstrated greater drug tolerance than cells in the stationary phase, which encompassed a substantial portion of persisters. Proteomic analysis revealed the up-regulation of proteins linked to host cell invasion and stress resistance, while proteins related to signaling and cellular processes were down-regulated. Fluorescence in situ hybridization (FISH) analysis confirmed that the VBNC state truly boosted B. henselae’s invasion of HUVECs. This study highlights B. henselae’s capacity to enter a VBNC state under thermal and antibiotic stress, emphasizing the urgent need for advanced diagnostic and therapeutic strategies to effectively target VBNC cells, which complicate diagnosis and treatment.
Evaluation of periodontitis parameters and plaque examination by microscopy: a report on 20 patients
Authors: Bonner Mark
Published: November 2024

The protozoan parasite Entamoeba gingivalis has long been detected in diseased gingival pockets. The parasite is found in 81% of diseased sites using PCR and in up to 100% using microscopy, whereas it is mostly absent from healthy gingival sites. The purpose of this study on 20 periodontitis patients was to analyze the characteristic biofilm using phase-contrast microscopy and evaluate the results of a novel antiparasitic, anti-inflammatory therapeutical approach. The therapeutic strategy, termed “Periodontal Healing Protocol Bonner Dunoyé” (PHPBD), is implemented in monthly appointments for 8 months, and a control visit at one year. It involves a disinfection protocol, subgingival calculus removal, patient training and the microscopic analysis of periodontal biofilm sulci. The practitioner also records bleeding on probing (BOP) and pocket depth (PD) to quantify healing. In all cases, the initial biofilm composed mainly of parasites, neutrophils, spirochetes, and other motile bacteria was progressively replaced by a white blood cell-free biofilm, consisting of motionless coccoid bacteria, filaments, and epithelial cells, indicative of healthy periodontium. Results were stable from month 8 to month 12. At one year, both BOP and PD values were greatly reduced (96%–100% decrease) compared to initial levels. The average sulcus clinical pocket healing toward the 1–3 mm PD group teeth was close to 99% overall patients. In conclusion, implementation of PHPBD appears to result in complete healing of periodontitis within 12 months, as determined by BOP, PD, biofilm microscopic monitoring and elimination of motile bacteria, parasites, and inflammatory cells. Thus, periodontal dysbiosis can be microscopically guided toward predictable eubiosis. Further studies are needed to evaluate long-term benefits.
Spatiotemporal evolution and optimization analysis of investment efficiency in China’s agricultural water conservancy infrastructure based on a two-stage DEA model
Authors: Sun Li , Zhang Chi , Liu Qingchun , Xu Changxin , Song Min
Published: November 2024

The turbulent international political and economic situation has presented significant challenges to food and water security. Agricultural water conservancy infrastructure has garnered considerable attention due to its crucial role in the allocation and efficient utilization of water resources. Traditional research on the investment efficiency of agricultural water conservancy infrastructure often treats the intermediate impact pathways as a “black box”, neglecting the distinctions among various links. This article employs a two-stage DEA model to partition the impact of agricultural water conservancy infrastructure investment on agricultural output into two stages: water supply and water use. Utilizing data of 31 provinces in China from 2008 to 2022, we measured the efficiency of the two stages, as well as the spatiotemporal distribution and evolution characteristics. The findings reveal a spatial misalignment between water supply and water use efficiency: regions exhibiting higher water supply efficiency in the first stage are primarily those with abundant water resource endowments, whereas water use efficiency in the second stage is closely linked to regional economic development levels. Additionally, the spatial distribution and evolution characteristics of efficiency values indicate that the polarization of water use efficiency is more pronounced, with a significant spatial correlation observed between geographically adjacent areas and those within the same watershed. Conversely, water supply efficiency shows a significant correlation only within the context of watershed relationships. Based on the analysis of the sources of efficiency loss, recommendations include increasing investment in water-saving irrigation technologies, developing agricultural water conservancy infrastructure suitable for large-scale mechanized production, and designing investment compensation mechanisms. Future research is suggested to use econometric models to further examine and identify factors affecting efficiency, particularly the impacts of inter-basin water transfer projects.
Clinicopathological characteristics, evolution, and treatment outcomes of hormone receptor-negative/HER2-low metastatic breast cancer: a pooled analysis of individual patient data from three prospective clinical trials
Authors: Hu Shihui , Zhao Yannan , Xie Yizhao , You Shuhui , Hu Xichun , Zhang Jian , Wang Leiping , Cao Jun , Gong Chengcheng , Wang Biyun
Published: November 2024

Objective

With the approval of trastuzumab deruxtecan for the treatment of unresectable/metastatic HER2-low breast cancer, human epidermal growth factor receptor 2 (HER2)-low has emerged as a clinically actionable biomarker. There is an urgent need for a deeper understanding of HER2-low breast cancer patients. Therefore, this study was conducted to explore the clinicopathological characteristics, the evolution of HER2-low status, and its impact on the prognosis of hormone receptor (HoR)-negative/HER2-low metastatic breast cancer (MBC) patients.

Methods

This pooled analysis included 350 metastatic triple-negative breast cancer (mTNBC) patients who received first-line platinum-based chemotherapy at Fudan University Shanghai Cancer Center from November 2007 to July 2022. Patients were categorized into HER2-0 and HER2-low groups based on their HER2 status. Baseline clinicopathological characteristics, evolution of HER2 status between primary and metastatic lesions, and treatment efficacy were compared between the two groups.

Results

Among the 350 mTNBC patients, 34.9% (122/350) were HER2-low and 65.1% (228/350) were HER2-0. Significant differences were observed between HER2-low and HER2-0 patients in terms of age and menopausal status. HER2-low patients were older (54 vs. 49 years, p=0.002) and had a lower proportion of premenopausal patients (32.8% vs. 52.6%, p<0.001) compared to HER2-0 patients. No significant differences were observed in progression-free survival (PFS) and overall survival (OS) between HER2-low and HER2-0 patients receiving first-line platinum-based chemotherapy (mPFS: 7.43 vs. 8.30 months, p=0.389, HR=1.11, 95% CI 0.88-1.40; mOS: 25.37 vs. 26.63 months, p=0.907, HR=1.02, 95% CI 0.76-1.37). Additionally, 32.3% (41/127) of patients exhibited discordant HER2 status between primary and metastatic lesions, primarily evolving from HER2-0 to HER2-low. Notably, patients with discordant HER2 status had significantly longer PFS compared to those with concordant status (mPFS: 11.07 vs. 7.53 months, p=0.020). The Cox multivariate analysis showed that HER2 status consistency (p=0.026) was an independent predictor of PFS.

Conclusion

In mTNBC patients, those with HER2-low status had similar responses to platinum-based chemotherapy as HER2-0 patients. There was significant discordance in HER2 status between primary and metastatic lesions. Patients with discordant HER2 status had better responses to platinum-based chemotherapy. Therefore, for patients with HER2-0 primary lesions, re-evaluation of HER2 status in metastatic lesions through biopsy may offer new treatment opportunities.
Growth hormone/insulin-like growth factor I axis in health and disease states: an update on the role of intra-portal insulin
Authors: Yuen Kevin C. J. , Hjortebjerg Rikke , Ganeshalingam Ashok Ainkaran , Clemmons David R. , Frystyk Jan
Published: November 2024

Growth hormone (GH) is the key regulator of insulin-like growth factor I (IGF-I) generation in healthy states. However, portal insulin delivery is also an essential co-player in the regulation of the GH/IGF-I axis by affecting and regulating hepatic GH receptor synthesis, and subsequently altering hepatic GH sensitivity and IGF-I generation. Disease states of GH excess (e.g., acromegaly) and GH deficiency (e.g., congenital isolated GH deficiency) are characterized by increased and decreased GH, IGF-I and insulin levels, respectively, where the GH/IGF-I relationship is reflected by a “primary association”. When intra-portal insulin levels are increased (e.g., obesity, Cushing’s syndrome, or due to treatment with glucocorticoids and glucagon-like peptide 1 receptor agonists) or decreased (e.g., malnutrition, anorexia nervosa and type 1 diabetes mellitus), these changes secondarily alter hepatic GH sensitivity resulting in a “secondary association” with discordant GH and IGF-I levels (e.g., high GH/low IGF-I levels or low GH/high IGF-I levels, respectively). Additionally, intra-portal insulin regulates hepatic secretion of IGFBP-1, an inhibitor of IGF-I action. Through its effects on IGFBP-1 and subsequently free IGF-I, intra-portal insulin exerts its effects to influence endogenous GH secretion via the negative feedback loop. Therefore, it is important to understand the effects of changes in intra-portal insulin when interpreting the GH/IGF-I axis in disease states. This review summarizes our current understanding of how changes in intra-portal insulin delivery to the liver in health, disease states and drug therapy use and misuse that leads to alterations in GH/IGF-I secretion that may dictate management decisions in afflicted patients.
Hyperactive browning and hypermetabolism: potentially dangerous element in critical illness
Authors: Huang Lu , Zhu Lili , Zhao Zhenxiong , Jiang Shenglu
Published: November 2024

Brown/beige adipose tissue has attracted much attention in previous studies because it can improve metabolism and combat obesity through non-shivering thermogenesis. However, recent studies have also indicated that especially in critical illness, overactivated brown adipose tissue or extensive browning of white adipose tissue may bring damage to individuals mainly by exacerbating hypermetabolism. In this review, the phenomenon of fat browning in critical illness will be discussed, along with the potential harm, possible regulatory mechanism and corresponding clinical treatment options of the induction of fat browning. The current research on fat browning in critical illness will offer more comprehensive understanding of its biological characteristics, and inspire researchers to develop new complementary treatments for the hypermetabolic state that occurs in critically ill patients.
Analysis of the impact of measurement on the impedance stability of HVDC grids
Authors: Zhao Dawei , Chen Zaiyu , Bu Jing , Li Qun , Li Qiang , Rong Xiaohu
Published: November 2024

The rapid advancement of power electronics technology has led to the development of more flexible DC transmission systems, revealing the issue of oscillations. In this paper, a novel impedance modeling approach for the DC side of Modular Multilevel Converters (MMCs) has been introduced to overcome the limitations of previous methods, which did not take into account the complete control structure and internal harmonic characteristics. An accurate impedance model has been established, integrating the types of disturbance signal sources used in impedance measurement and the impact of measurement device characteristics on model accuracy. This approach enhances the precision of impedance stability analysis, which is crucial for system stability. The model’s effectiveness has been validated through simulations, demonstrating the influence of disturbance signal types and measurement device characteristics on the impedance stability of the MMC’s DC side.
Mendelian randomization and bioinformatics unveil potential links between gut microbial genera and colorectal cancer
Authors: Wu Long , Wu Huan , Huang Fei , Mu Song , Li Xiao-Yun , Zhang Bao-Fang , Zhen Yun-Huan , Li Hai-Yang
Published: November 2024

Background

Colorectal cancer (CRC) poses a significant global health burden, with high incidence and mortality rates. Despite advances in diagnostic and therapeutic modalities, early diagnosis remains critical for improved outcomes. Recent research has realized the important role of gut microbiota in CRC development, highlighting the need to elucidate potential relationships.

Methods

In this study, we employed Mendelian randomization (MR) to establish a robust potential link between gut microbial genera and CRC. Data from the MiBioGen database provided curated genome-wide association study (GWAS) summary datasets for microbial genera, while the Finngen database provided CRC outcome data. Instrumental variables (IVs) were identified based on genetic variants associated with gut microbiota. Various MR methods, including Inverse Variance Weighted (IVW), Weighted Median, Weighted Mode, Simple Mode, and MR-Egger, were employed to estimate potential effects. Functional analysis of genes near single nucleotide polymorphisms (SNPs) was performed to unravel potential pathways.

Results

Analysis of microbial genera identified five potentially associated with CRC: Eubacterium fissicatena group, Anaerofilum, Defluviitaleaceae UCG011, Ruminococcus 2, and Sutterella. Notably, Defluviitaleaceae UCG011 emerged as the only risk factor. Gene analysis revealed hub genes PTPRD and DSCAM near Defluviitaleaceae UCG011 associated SNPs. Expression analysis showed that PTPRD decreased in colon cancer and DSCAM decreased in rectal cancer. The methylation status of the PTPRD gene promoter region indicated potential regulatory alterations.

Conclusion

This study establishes a potential relationship between five specific gut microbial genera, particularly Defluviitaleaceae UCG011, and CRC. Hub genes PTPRD and DSCAM provide insights into potential molecular mechanisms, suggesting the potential role of Defluviitaleaceae UCG011 in modulating the initiation and progression of CRC. Further research is essential to validate these associations and delve deeper into therapeutic implications.
Disrupted balance between pro-inflammatory lipid mediators and anti-inflammatory specialized pro-resolving mediators is linked to hyperinflammation in patients with alcoholic hepatitis
Authors: Li Wei , Xia Ying , Yang Jing , Sanyal Arun J. , Shah Vijay H. , Chalasani Naga P. , Yu Qigui
Published: November 2024

Background

Alcoholic hepatitis (AH) is characterized by intense systemic and liver inflammation, posing significant risks of health complications and mortality. While inflammation is a crucial defense mechanism against injury and infection, its timely resolution is essential to prevent tissue damage and restore tissue homeostasis. The resolution of inflammation is primarily governed by specialized pro-resolving mediators (SPMs), lipid metabolites derived from w-6 and w-3 poly-unsaturated fatty acids (PUFAs). Currently, the balance between pro-inflammatory lipid mediators (PLMs) and SPMs in the w-6 and w-3 PUFA metabolic pathways and the impact of alcohol abstinence on profiles of PLMs and SPMs in AH patients are not well studied.

Methods

In this study, we used LC-MS/MS and ELISA to quantify levels of lipid mediators (LMs) and their precursors in the plasma samples from 58 AH patients, 29 heavy drinkers without overt liver diseases (HDCs), and 35 healthy controls (HCs). Subsequently, we assessed correlations of altered LMs with clinical parameters and inflammatory mediators. Furthermore, we conducted a longitudinal study to analyze the effects of alcohol abstinence on LMs over 6- and 12-month follow-ups.

Results

AH patients exhibited significantly higher plasma levels of w-6 PLMs (PGD2 and LTB4) and SPM RvE1 compared to HDCs or HCs. Conversely, the SPM LXA4 was significantly downregulated in AH patients. Some of these altered LMs were found to correlate with AH disease severity and various inflammatory cytokines. Particularly, the LTB4/LXA4 ratio was substantially elevated in AH patients relative to HDCs and HCs. This altered ratio displayed a positive correlation with the MELD score. Importantly, the majority of dysregulated LMs, particularly PLMs, were normalized following alcohol abstinence.
Neuronal substance P-driven MRGPRX2-dependent mast cell degranulation products differentially promote vascular permeability
Authors: Nagamine Masakazu , Kaitani Ayako , Izawa Kumi , Ando Tomoaki , Yoshikawa Akihisa , Nakamura Masahiro , Maehara Akie , Yamamoto Risa , Okamoto Yoko , Wang Hexing , Yamada Hiromichi , Maeda Keiko , Nakano Nobuhiro , Shimizu Toshiaki , Ogawa Hideoki , Okumura Ko , Kitaura Jiro
Published: November 2024

Mas-related G protein-coupled receptor b2 (Mrgprb2) binding to its cationic endogenous and exogenous ligands induces mast cell degranulation and promotes inflammation in mice. However, the physiological roles of its human homologue MRGPRX2 remain unclear. Here we aimed to elucidate the mechanisms by which MRGPRX2 regulates vascular permeability, and generated MRGPRX2 knock-in (MRGPRX2-KI) and Mrgprb2 knockout (Mrgprb2-KO) mice. Substance P (SP) and ciprofloxacin strongly degranulated MRGPRX2-KI peritoneal mast cells (PMCs) better than WT PMCs, whereas Dermatophagoides pteronyssinus (Der p) extract and phenol-soluble modulin α3 (PSMα3) did not degranulate PMCs. SP-stimulated MRGPRX2-KI PMCs released large amounts of histamine and mast cell protease 4 (MCPT4) chymase. Der p extract, PSMα3, and MCPT4, but not histamine, induced SP release from dorsal root ganglion (DRG) cells. However, this effect of Der p extract/PSMα3 was suppressed by a transient receptor potential vanilloid 1 (TRPV1) antagonist. SP-, ciprofloxacin-, Der p extract-, PSMα3-, and MCPT4-induced vascular permeability was highest in MRGPRX2-KI mice, which depended on SP. In addition, SP-, ciprofloxacin- and PSMα3-induced MRGPRX2-dependent vascular hyperpermeability was suppressed by antihistamine and chymase inhibitor. TRPV1 antagonist also inhibited PSMα3-induced MRGPRX2-dependent vascular hyperpermeability. Both Mrgprb2-KO and MRGPRX2-KI did not influence the histamine-induced murine vascular hyperpermeability. Overall, our results suggest that neuronal SP induces MRGPRX2-dependent mast cell degranulation, releasing histamine and chymase, which promote vascular hyperpermeability directly or indirectly via DRG cell activation. Importantly, the worsening cycle (MRGPRX2 → mast cell degranulation → chymase → DRG activation → SP → MRGPRX2) seems to play an important role in human MRGPRX2-depdendent inflammation.
Identification of key biomarkers related to fibrocartilage chondrocytes for osteoarthritis based on bulk, single-cell transcriptomic data
Authors: Pan Bailin , Yao Peixiu , Ma Jinjin , Lin Xuanhao , Zhou Laixi , Lin Canzhen , Zhang Yufeng , Lin Bendan , Lin Chuangxin
Published: November 2024

Introduction

Osteoarthritis (OA) is a prevalent joint disease that severely impacts patients’ quality of life. Due to its unclear pathogenesis and lack of effective therapeutic targets, discovering new biomarkers for OA is essential. Recently, the role of chondrocyte subpopulations in OA progression has gained significant attention, offering potential insights into the disease. This study aimed to explore the role of fibrocartilage chondrocytes (FC) in the progression of OA and identify key biomarkers related to FC.

Methods

We analyzed single-cell ribonucleic acid sequencing (scRNA-seq) data from samples of OA and normal cartilage, focusing on FC. Microarray data were integrated to identify differentially expressed genes (DEGs). We conducted functional-enrichment analyses, including Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO), and used weighted gene co-expression network analysis (WGCNA) and the least absolute shrinkage and selection operator (LASSO) algorithm to select biomarkers. A novel risk model for OA was constructed using these biomarkers. We then built a transcription factor (TF)–gene interaction network and performed immunohistochemistry (IHC) to validate protein expression levels of these biomarkers in cartilage samples.

Results

The study identified 545 marker genes associated with FC in OA. GO and KEGG analyses revealed their biological functions; microarray analysis identified 243 DEGs on which functional-enrichment analysis were conducted. Using WGCNA and LASSO, we identified six hub genes, on the basis of which we constructed a risk model for OA. In addition, correlation analysis revealed a close association between Forkhead Box (FoxO)-mediated transcription and these these biomarkers. IHC showed significantly lower protein levels of ABCA5, ABCA6 and SLC7A8 in OA samples than in normal samples.

Conclusion

This study used a multi-omics approach to identify six FC-related OA biomarkers (BCL6, ABCA5, ABCA6, CITED2, NR1D1, and SLC7A8) and developed an exploratory risk model. Functional enrichment analysis revealed that the FoxO pathway may be linked to these markers, particularly implicating ABCA5 and ABCA6 in cholesterol homeostasis within chondrocytes. These findings highlight ABCA family members as novel contributors to OA pathogenesis and suggest new therapeutic targets.
Improvements and characterization of a microcosmic-based device for sediment erosion
Authors: Shao Yuyang , Zhang Zhongao , Tang Yulin , Zhang Wei , Maa Jerome P. -Y.
Published: November 2024

Effective management of sediment transport in water bodies is crucial for maintaining navigational channels and reducing siltation in harbors. This study introduces the first effort in the development of a Field Instrument for Measuring Bed Erosion Response based on microcosmic analysis. The device is designed to automatically measure the sediment bed responses under a series of selected bed shear stresses to determine the critical bed shear stresses for sediment incipient motion and the erosion rates for selected excess bed shear stresses. Numerical simulations were conducted using computational fluid dynamics software (FLUENT) to ensure a reasonable and uniform distribution of the selected bed shear stress across the sediment bed. A lab version of this device was also built and tested using granular sandy sediments and the results were validated against the Shields curve for incipient motion. During this test, however, a problem of sandy bed liquefaction at the bed center was identified because of the pumping out of water at top center. For enhancing the device performances, modifications of the pumping intake were made to eliminate the liquefaction problem. Further a lab test on kaolinite beds with three consolidated durations also demonstrated the capability of this improved device. The study confirms the basic design parameters of the improved device, including the optimal rotating speeds of its pump and disk motors, which are critical for achieving the desired erosion dynamics. These results highlight the potential of the device to significantly improve the precision and efficiency of sediment transport studies in natural aquatic environments.
Knowledge, attitude, and practice of psoriatic arthritis among patients with psoriasis
Authors: Mei Aihua , Luan Mei , Li Pan , Chen Jun , Mou Kuanhou
Published: November 2024

Introduction

This study aimed to investigate the knowledge, attitude and practice (KAP) of psoriatic arthritis among patients with psoriasis. The KAP questionnaire is a widely used tool in public health research, designed to assess individuals’ understanding (knowledge), beliefs (attitude), and behaviors (practice) related to a specific health condition.

Methods

A cross-sectional study was conducted at Sinopharm Dongfeng General Hospital from September to November 2023. Demographic information and KAP scores were assessed using a structured questionnaire, which evaluated patient knowledge about psoriatic arthritis, their attitude toward managing it, and their practical engagement in preventive or treatment behaviors.

Results

In this study, 392 valid questionnaires were analyzed. Of these, 290 respondents (74.0%) were male, and 296 (75.5%) reported no comorbid conditions. The median scores for knowledge, attitude, and practice were 8 (interquartile range [IQR]: 6–10), 21 (IQR: 19–24), and 14 (IQR: 8–22), respectively. Multivariate logistic regression analysis indicated that practice was independently associated with being female (OR = 0.426, 95% CI: 0.259–0.703, p = 0.001), being aged 30–39 years (OR = 2.159, 95% CI: 1.223–3.811, p = 0.008) or 40–49 years (OR = 2.002, 95% CI: 1.019–3.936, p = 0.044), having a Dermatology Life Quality Index (DLQI) score of 11–30 (OR = 2.569, 95% CI: 1.158–5.700, p = 0.020), and not having psoriatic arthritis (OR = 0.300, 95% CI: 0.168–0.537, p < 0.001).

Conclusion

Patients with psoriasis had suboptimal knowledge, positive attitude and inactive practice toward psoriatic arthritis. To address this, healthcare providers should prioritize educational interventions, with a specific focus on younger patients, females, and individuals with a higher DLQI score, to enhance awareness and promote proactive management of psoriatic arthritis among this patient population.
Genetic association of type 2 diabetes and antidiabetic drug target with skin cancer
Authors: Zhao Juyan , Zhang Yu , Li Jianbo , Li Qi , Teng Ziyue
Published: November 2024

Background

Several observational studies have suggested that type 2 diabetes (T2D) is a risk factor for skin cancer, and antidiabetic drugs may reduce skin cancer risk. Nevertheless, the findings remain ambiguous. This Mendelian randomization (MR) study aimed to investigate the causal association of T2D with skin cancer and evaluate the potential impact of antidiabetic drug targets on skin cancer.

Methods

Genetic variants associated with glycated hemoglobin (HbA1c), Type 2 Diabetes (T2D), and antidiabetic drug targets (KCNJ11, ABCC8, PPARG, INSR, GLP1R, SLC5A2, and DPP4) were sourced from genome-wide association studies in the UK Biobank and the DIAMANTE consortium. Genetic summary statistics on skin cancer were obtained from the FinnGen consortium. MR analysis was primarily performed leveraging the inverse-variance weighted method, with additional sensitivity analyses conducted. Summary data-based MR (SMR) was utilized to further investigate the association between antidiabetic drug target gene expression and skin cancer. Colocalization analysis was carried out to verify the robustness of the results.

Results

Genetically proxied elevated levels of HbA1c were found to be suggestively associated with a reduced risk of melanoma (OR: 0.886, 95% confidence interval (CI): 0.792–0.991, p = 0.0347). Additionally, genetically proxied T2D was notably associated with a lower risk of basal cell carcinoma (OR: 0.960, 95% CI: 0.928–0.992, p = 0.0147). The study also discovered that perturbation of the antidiabetic drug target SLC5A2 was significantly associated with an increased risk of basal cell carcinoma (for SLC5A2 perturbation equivalent to a 6.75 mmol/mol decrement in HbA1c: OR: 2.004, 95% CI: 1.270–3.161, p = 0.0027). However, this finding was not supported by colocalization analysis. Notably, no other drug target perturbations were found to be associated with skin cancer. Furthermore, SMR analysis failed to detect an association between antidiabetic drug target genes and skin cancer.

Conclusion

The study suggests that higher HbA1c levels and T2D may be associated with a reduced risk of skin cancer. However, the results did not provide evidence to support the association between antidiabetic drug targets and skin cancer. Further evaluation of these drug targets is required to confirm the findings in this analysis.
Prevalence of bronchiectasis in inflammatory bowel disease: a systematic review and meta-analysis
Authors: Ma Yu , Qiang Zhihui , Zhou Miaomiao , Zhang Tianyi , Li Zhuoyang , Zhong Haicheng , Chang Yue , Ning Zimeng , Liu Yun
Published: November 2024

Objective

The aim of this study was to conduct a systematic review and meta-analysis of the incidence of inflammatory bowel disease-associated bronchiectasis (IBD-BE) and to explore the possible risk factors for IBD-BE, which could help to understand the pulmonary involvement in patients with IBD and to determine the global incidence of the disease.

Methods

We searched PubMed and EMBASE databases to identify information on the prevalence of IBD-BE among IBD patients in the published literature. Information was extracted on study design, country, year, IBD-BE testing method, IBD characteristics, number of IBD-BE cases and total number of IBD patients, and factors associated with IBD-BE. We conducted meta-analyses using random-effects or fixed-effects models to estimate the prevalence of IBD-BE among IBD patients.

Results

Out of a total of 682 studies, we identified 16 studies that reported prevalence. These studies used a heterogeneous approach to identify IBD-BE. In these 16 studies, there were 92,191 patients with IBD, of whom 372 cases of IBD-BE were identified. The results of the meta-analysis showed that the overall prevalence of IBD-BE in IBD derived from the use of a random effects model was 5.0% (95% CI 2.0–12.0%). In contrast, the prevalence of IBD-BE in studies using high-resolution chest computed tomography (HRCT) imaging was 12% (95% CI 4–39%) using a random-effects model. When only retrospective studies with sample sizes greater than 100 (n = 6) were considered, the prevalence was 1% (95% CI 0–1%). However, when only retrospective studies with sample sizes less than 100 were included (n = 4), the prevalence was 29% (95% CI 6–100%); in prospective studies (n = 6), the combined prevalence was 11% (95% CI 4–29%). we performed a subgroup analysis of the differences in the incidence of IBD-BE between the different studies, each of which we subgrouped by type of study, type of disease, duration of disease, and diagnostic modality, and the results showed no significance. Future studies should standardize methods to identify IBD-BE cases and investigate the natural history and clinical course given the relatively high prevalence among IBD.

Conclusion

In this systematic review and meta-analysis, the prevalence of IBD-BE was 12% among studies with HRCT imaging, suggesting that bronchiectasis may be an underestimated common extraintestinal manifestation of IBD. Asymptomatic patients with IBD-BE may present with abnormalities on HRCT or pulmonary function tests. Future studies should standardize methods to identify IBD-BE cases and investigate the natural history and clinical course given the relatively high prevalence among IBD.
Riverine bacterial communities are more shaped by species sorting in intensive urban and agricultural watersheds
Authors: She Yuanyang , Wang Peng , Wen Jiawei , Ding Mingjun , Zhang Hua , Nie Minghua , Huang Gaoxiang
Published: November 2024

Bacterial communities play a crucial role in maintaining the stability of river ecosystems and driving biogeochemical cycling, exhibiting high sensitivity to environmental change. However, understanding the spatial scale effects and assembly mechanisms of riverine bacterial communities under distinct anthropogenic disturbances remains a challenge. Here, we investigated bacterial communities across three distinct watersheds [i.e., intensive urban (UW), intensive agricultural (AW), and natural (NW)] in both dry and wet seasons. We explored biogeographic patterns of bacterial communities and the influence of landscape patterns at multi-spatial scales and water chemistry on bacterial communities. Results showed that α diversity was significantly lower in UW and AW compared to NW, particularly in the dry season. A gradient of β diversity with NW > UW > AW was observed across both seasons (p < 0.05). Pseudomonadota, Bacteroidota, and Actinobacteriota were the most abundant phyla across all watersheds, with specific taxa enriched in each watershed (i.e., the class Actinobacteria was significant enrichment in UW and AW, and Clostridia in NW). The influence of landscape patterns on bacterial communities was significantly lower in human-disturbed watersheds, particularly in UW, where this influence also varied slightly from near riparian buffers to sub-watershed. Homogeneous selection and drift jointly dominated the bacterial community assembly across all watersheds, with homogeneous selection exhibiting a greater influence in UW and AW. Landscape patterns explained less variance in bacterial communities in UW and AW than in NW, and more variance was explained by water chemistry (particularly in UW). These suggest that the stronger influence of species sorting in UW and AW was driven by more allochthonous inputs of water chemistry (greater environmental stress). These findings provide a theoretical foundation for a deeper understanding of riverine bacterial community structure, spatial scale effects, and ecological management under different anthropogenic activities.
A review of key microbial and nutritional elements for mechanistic modeling of rumen fermentation in cattle under methane-inhibition
Authors: Pressman Eleanor M. , Kebreab Ermias
Published: November 2024

The environmental impacts of livestock agriculture include the production of greenhouse gasses (GHG) such as methane (CH4) through enteric fermentation. Recent advances in our understanding of methanogenesis have led to the development of animal feed additives (AFA) that can reduce enteric CH4 emissions. However, many interacting factors impact hydrogen (H2) and CH4 production and AFA efficacy, including animal factors, basal diet, particle and fluid outflow, microbial populations, rumen fluid pH, and fermentative cofactor dynamics. Characterizing the response of rumen fermentation to AFA is essential for optimizing AFA implementation. Mechanistic models of enteric fermentation are constructed to represent physiological and microbial processes in the rumen and can be updated to characterize the dependency of AFA efficacy on basal diet and the impacts of AFA on fermentation. The objective of this article is to review the current state of rumen mechanistic modeling, contrasting the representation of key pools in extant models with a particular emphasis on representation of CH4 production. Additionally, we discuss the first rumen mechanistic models to include AFA and emphasize future model needs for improved representation of rumen dynamics under CH4-inhibition due to AFA supplementation, including the representation of microbial populations, rumen pH, fractional outflow rates, and thermodynamic control of fermentative pathways.
Polymerization potential of a bacterial CotA-laccase for β-naphthol: enzyme structure and comprehensive polymer characterization
Authors: Refaat Marina , ElRakaiby Marwa T. , El Hariri El Nokab Mustapha , Es Sayed Julien , Elshewy Ahmed , Sebakhy Khaled O. , Moneib Nayera , Wang Tuo , Smith Thomas J. , Habib Mohamed H.
Published: November 2024

Introduction

Laccases are blue-multicopper containing enzymes that are known to play a role in the bioconversion of recalcitrant compounds. Their role in free radical polymerization of aromatic compounds for their valorization remains underexplored. In this study, we used a pBAD plasmid containing a previously characterized CotA laccase gene (abbreviated as Bli-Lacc) from Bacillus licheniformis strain ATCC 9945a to express this enzyme and explore its biotransformation/polymerization potential on β-naphthol.

Methods

The protein was expressed from TOP10 cells of Escherichia coli after successful transformation of the plasmid. Immobilized metal affinity chromatography (IMAC) was used to generate pure protein. The biocatalytic polymerization reaction was optimized based on temperature, pH and starting enzyme concentration. 1H and 13C solution nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FTIR), and solid-state NMR (ssNMR) were used to characterize the formed polymer. A one-gram conversion reaction was done to explore applicability of the reaction in a pilot-scale.

Results

The polymerization reaction generated a brown precipitate, and its chemical structure was confirmed using 1H and 13C NMR and FTIR. SsNMR revealed the presence of two different orientational hydroxyl functional groups in the polymer in addition to the presence of a very small amount of ether linkages (< 2%). This analysis elucidated that polymerization occurred mainly on the carbons of the aromatic rings, rather than on the carbons attached to the hydroxyl groups, resulting in a condensed ring or polynuclear aromatic structure. The reaction was optimized, and the highest yield was attained under conditions of 37°C, pH 10 and a starting enzyme concentration of 440 nM in 50 mM phosphate buffer. A one-gram conversion yielded 216 mg of polymer as dry mass. The crystal structure of the enzyme was solved at 2.7 Å resolution using X-ray crystallography and presented with a hexagonal space group. The final structure was deposited in the Protein Databank (PDB) with an ID−9BD5.

Discussion

This article provides a green/enzymatic pathway for the remediation of phenolics and their valorization into potential useful polymeric materials. The comprehensive analysis of the formed polymer provides insight into its structure and functional moieties present. Based on the yield of the one-gram conversion, this synthetic method proves useful for a pilot-scale production level and opens opportunities to invest in using this polymer for industrial/environmental applications.
Risk factors analysis of cognitive frailty among geriatric adults in nursing homes based on logistic regression and decision tree modeling
Authors: Gao Jing , Bai Dingxi , Chen Huan , Chen Xinyu , Luo Huan , Ji Wenting , Hou Chaoming
Published: November 2024

Objective

To investigate the risk factors associated with cognitive frailty among older adults in nursing homes using logistic regression and decision tree modeling, and to compare the predictive performance of these methods.

Methods

A cross-sectional study was conducted involving 697 participants aged 60 and older residing in eight nursing homes in Sichuan province, China. Participants were recruited using convenience sampling. Data were collected through questionnaires administered to the older adults. Logistic regression and decision tree modeling were employed to construct models predicting cognitive frailty.

Results

Logistic regression analysis identified age, education degree, exercise, intellectual activities, number of chronic diseases, nutritional status, sleep quality, and depression as significant predictors of cognitive frailty (all p < 0.05). The final decision tree model consisted of three layers and 17 nodes. Six factors were identified as significant predictors: sleep quality, number of chronic diseases, depression, education level, nutrition, and exercise. Receiver operating characteristic (ROC) curve analysis revealed that the area under the curve (AUC) for the logistic regression model was 0.735 (95% CI: 0.701–0.767) with a sensitivity of 0.58 and specificity of 0.75. The AUC for the decision tree model was 0.746 (95% CI: 0.712–0.778) with a sensitivity of 0.68 and specificity of 0.70.

Conclusion

Age, education level, exercise, intellectual activities, sleep quality, number of chronic diseases, nutritional status, and depression are significant risk factors for cognitive frailty in older adults residing in nursing homes. Both logistic regression and decision tree models demonstrated comparable predictive performance, with each offering distinct advantages. The combined use of these methods can enhance predictive accuracy and provide valuable insights for clinical practice and policy development.
MAM-mediated mitophagy and endoplasmic reticulum stress: the hidden regulators of ischemic stroke
Authors: Jia Ziyi , Li Hongtao , Xu Ke , Li Ruobing , Yang Siyu , Chen Long , Zhang Qianwen , Li Shulin , Sun Xiaowei
Published: November 2024

Ischemic stroke (IS) is the predominant subtype of stroke and a leading contributor to global mortality. The mitochondrial-associated endoplasmic reticulum membrane (MAM) is a specialized region that facilitates communication between the endoplasmic reticulum and mitochondria, and has been extensively investigated in the context of neurodegenerative diseases. Nevertheless, its precise involvement in IS remains elusive. This literature review elucidates the intricate involvement of MAM in mitophagy and endoplasmic reticulum stress during IS. PINK1, FUNDC1, Beclin1, and Mfn2 are highly concentrated in the MAM and play a crucial role in regulating mitochondrial autophagy. GRP78, IRE1, PERK, and Sig-1R participate in the unfolded protein response (UPR) within the MAM, regulating endoplasmic reticulum stress during IS. Hence, the diverse molecules on MAM operate independently and interact with each other, collectively contributing to the pathogenesis of IS as the covert orchestrator.