bluuebunny/crossref_metadata_2025 huggingface dataset:
Energy Policy until 2050—Comparative Analysis between Poland and Germany
Authors: Wojtaszek Henryk , Miciuła Ireneusz , Modrzejewska Dagmara , Stecyk Adam , Sikora Mariusz , Wójcik-Czerniawska Agnieszka , Smolarek Małgorzata , Kowalczyk Anna , Chojnacka Małgorzata
Published: January 2024

The article presents a comprehensive study of the energy strategies of both countries, aimed at achieving their goals by 2050. A literature review presents global trends in energy policy, the current situation in Poland and Germany, and the importance of sustainable energy. For Poland and Germany, the current situation, goals, and strategies for 2050 are described, and the results of surveys carried out using a survey questionnaire are presented. A comparative analysis included a comparison of the goals and strategies of both countries and the results of surveys, presented in tabular form. This analysis drew conclusions regarding the differences and similarities in the approach of both countries to energy policy. The “Challenges and Prospects” section identifies potential challenges and presents opportunities and recommendations for the future. In conclusion, the article provides a thorough analysis, based on the methodology of a literature review, survey questionnaire, and tabular analysis, and contains important conclusions and implications for energy policy in Poland and Germany. The article also addresses the limitations of the study that may affect the interpretation of the results.
Genome-Wide Analysis of Homologous E6-AP Carboxyl-Terminal E3 Ubiquitin Ligase Gene Family in Populus trichocarpa
Authors: Fu Yanrui , Li Hui , Li Yaqi , Zhao Haoqin , Yang Da , Chen Aihua , Yang Jingli
Published: January 2024

Proteins containing the homologous E6-AP carboxyl-terminal (HECT) domain are a class of E3 ubiquitin ligases involved in the ubiquitin–proteasome pathway, which plays an irreplaceable role in plant growth, development, and stress resistance. However, a phylogenetic analysis and expression profile of the HECT gene (PtrHECT) in the model plant Populus trichocarpa (Torr. & Gray) have not been reported. In this study, we identified 13 PtrHECT genes using genome-wide analysis, and then these were divided into four groups. The protein interaction networks showed that the PtrHECT protein may interact with PTR6 and participate in ABA signal regulation. Abiotic stress is the main environmental factor limiting plant growth and development. The qRT-PCR results showed that PtrHECT1, 4, 7, 8, and 9 were significantly up-regulated in leaves at each time point under drought stress, and most PtrHECT genes responded to both drought and high salt stress, consistent with their promoter sequence analysis, revealing the presence of an important number of phytohormone-responsive and stress-related cis-regulatory elements. This study provides useful information for further analysis of the functions of the HECT gene family in P. trichocarpa.
Mating System Analysis and Genetic Diversity of Parkia multijuga Benth. One Native Tree Species of the Amazon
Authors: Oliveira Ariane Mendes , Ramos Santiago Linorio Ferreyra , Ferreira Marciel José , Lopes Ricardo , Meneses Carlos Henrique Salvino Gadelha , Valente Magno Savio Ferreira , da Silva Rogério Freire , Batista Jacqueline da Silva , Muniz Aleksander Westphal , Lopes Maria Teresa Gomes
Published: January 2024

The Amazonian native tree species Parkia multijuga has potential silvicultural characteristics that can be utilized to productive plantations. Understanding its mating system is necessary to delineate the methods for the breeding of the species, the collection of seeds for conservation, and the use of seedlings for production plantations. The aim of this study is to evaluate the mating system and population genetic diversity of P. multijuga, using molecular markers. The DNA of 221 plants was extracted and genotyped with nine microsatellite loci using capillary electrophoresis in an automated DNA sequencer. The estimates for single and multilocus crossing rates were 0.998 and 1.0, respectively. The paternity correlation was low (r^p(m) = 0.307). The fixation index (f) showed values below zero, indicating an excess of heterozygotes. The cluster number K = 2 shows a better grouping among families for genetic structure. P. multijuga families consist mainly of half-sibs, and the reproductive strategy of the species is allogamy.
Research on the Wood Density Measurement in Standing Trees through the Micro Drilling Resistance Method
Authors: Yao Jianfeng , Zhao Yabin , Lu Jun , Liu Hengyuan , Wu Zhenyang , Song Xinyu , Li Zhuofan
Published: January 2024

To achieve a micro-destructive and rapid measurement of the wood density of standing trees, this study investigated the possibility of the unified modeling of multiple tree species, the reliability of the micro drilling resistance method for measuring wood density, the relationship between drilling needle resistance and wood density, and whether moisture content has a significant impact on the model. First, 231 tree cores and drill resistance data were sampled from Pinus massoniana, Cunninghamia lanceolate, and Cryptomeria fortunei. The basic density and moisture content of each core were measured, and the average value of each resistance data record was calculated. Second, the average drill resistance, the natural logarithm of average drill resistance, and absolute moisture content were used as independent variables, while the basic wood density was used as the dependent variable. Third, the total model of the three tree species and sub-model for each tree species were established through a stepwise regression method. Finally, the accuracy of each model was compared and analyzed with that of using the average basic density of each tree species as an estimated density. The estimated accuracy of the total model, sub model, and average wood density modeling data were 90.070%, 93.865%, and 92.195%, respectively. The results revealed that the estimation accuracy of the sub-model was 1.670 percentage points higher than that of the average wood density modeling data, while the estimation accuracy of the total model was 2.125 percentage points lower than that of the average wood density modeling data. Additionally, except for Cryptomeria fortunei, the natural logarithm of drill resistance significantly influenced the wood density model at a significance level of 0.05. Moreover, moisture content significantly affected the total model and sub-models of Pinus massoniana at a significance level of 0.05. The results indicated the feasibility of using the micro-drilling resistance method to measure the wood density of standing trees. Moreover, the relationship between wood density and drill resistance did not follow a linear pattern, and moisture content slightly influenced the drill needle resistance. Furthermore, the establishment of a mathematical model for each tree species was deemed essential. This study provides valuable guidance for measuring the wood density of standing trees through the micro-drilling resistance method.
Characteristics of Forest Windthrow Produced in Eastern Carpathians in February 2020
Authors: Ciocirlan Mihai , Câmpu Vasile Răzvan
Published: January 2024

Windthrow is a phenomenon that causes major changes to tree stand evolution by blowing down or breaking either isolated trees or entire tree stands, with a strong ecological, social and economic impact. Both scattered and large-scale windthrow occurred in spruce (Picea abies (L.) Karst.) tree stands of Romania. They affected surfaces of various dimensions from harvestable forests. Such a phenomenon took place in the Curvature Carpathians in February 2020. Large-scale windthrow occurred in this area in 1995 as well, in the upper watershed of Bâsca river. Using the climate data from February 2020, this paper aims to identify the manner in which factors such as climate and site conditions together with tree stand characteristic and the anthropogenic factor impacted and influenced the occurrence of windthrow. The results showed that the intensity of this phenomenon had maximum effects when the wind coming from north/northeast reached the maximum speed of 32 m·s−1. Pure spruce tree stands situated on slopes with an inclination between 16 and 30° were mainly affected. Their position was counter to the wind direction, at an altitude between 1300 and 1500 m, on cambisols and spodosols. The analysis and statistical interpretation of data in the case of scattered and large-scale windthrow from the two management units showed that the same factors studied influence the variation of windthrow intensity in a different manner, or sometimes they do not influence it at all or they can only account for a small part of this variation.
Sustainable Supercapacitor Electrode Based on Activated Biochar Derived from Preserved Wood Waste
Authors: Huang Meiling , Dai Boren , Shi Jiangtao , Li Jiayao , Xia Changlei
Published: January 2024

Due to the inherent metals (Cu, As and Cr) in preserved wood waste (CCA-treated wood waste) that pose a risk to both the environment and human health, it is crucial to dispose of CCA-treated wood properly. Carbon materials have received widespread attention for their high porosity, renewability and simplicity of fabrication. This work presents a simple and effective process for producing carbon materials from leftover CCA-treated wood (chromated copper arsenate). Utilizing CCA-treated wood derived carbon (CCA-BC) and activating it with KOH (CCA-AC), electrode materials for supercapacitor applications were created and its electrochemical characteristics were investigated. The resulting material combines the conductivity of the metal in preserved wood with the good porosity provided by carbon materials. Compared with common wood biomass, carbon (W-BC) and common wood activated carbon (W-AC), CCA-BC and CCA-AC have better electrochemical properties. After being pyrolyzed at 600 °C for two hours, CCA-AC performed optimally electrochemically in 1 M Na2SO4 electrolyte, demonstrating a 72% capacity retention rate after 2000 charge and discharge cycles and a specific capacity of 76.7 F/g. This study provides a novel approach for the manufacture of supercapacitor electrodes, which also allows preserved wood waste an environmentally nondestructive form of elimination.
Modal Variability of Ginkgo Seed–Stem System Based on Model Updating
Authors: Zhou Jie , Xu Linyun , Zhou Hongping , Zhang Rongshan , Jia Zhicheng , Zhang Fubao , Zhang Yue , Chen Juan , Zhang Cheng
Published: January 2024

An accurate simulation model is crucial for the analysis of the correct modal information of the ginkgo seed–stem system (ginkgo subsystem). This underpins the provision of technical rationale for efficient and low-damage precision vibrational harvesting operations in ginkgo cultivation. In this study, based on the modal parameters of the ginkgo subsystem, a finite element model updating method is proposed to correct the elastic modulus of the stem with the natural frequency of the first bending mode. The large difference in the modal results calculated before and after model updating reveals that model updating is a critical step in the finite element analysis of crop subsystems. Then, an uncertainty parameter modeling method is proposed to investigate the modal variability of the ginkgo subsystem by finite element analysis. The results show that the stem length is a key parameter affecting the variability of natural frequencies, and the seed weight is a minor parameter. The variability of the ginkgo seed’s gravity center offset has a negligible effect on the natural frequencies of the system. The first natural frequency of the ginkgo subsystem can be utilized for vibrational harvesting. In addition, since the difference between the upper and lower limits of the first natural frequency of the ginkgo subsystem does not exceed 1 Hz, a specific excitation frequency can cause most ginkgo subsystems to resonate. This result facilitates the determination of precise excitation frequencies for efficient and low-damage ginkgo vibrational harvesting, ensuring both economic and ecological benefits in the management of ginkgo plantations.
Digital Communication and Social Organizations: An Evaluation of the Communication Strategies of the Most-Valued NGOs Worldwide
Authors: Moreno-Cabanillas Andrea , Castillero-Ostio Elizabet , Castillo-Esparcia Antonio
Published: January 2024

The communication of organizations with their audiences has undergone changes thanks to the Internet. Non-Governmental Organizations (NGOs), as influential groups, are no exception, as much of their activism takes place through grassroots digital lobbying. The consolidation of Web 2.0 has not only provided social organizations with a new and powerful tool for disseminating information but also brought about significant changes in the relationship between nonprofit organizations and their diverse audiences. This has facilitated and improved interaction between them. The purpose of this article is to analyze the level of interactivity implemented on the websites of leading NGOs worldwide and their presence on social networks, with the aim of assessing whether these influential groups are moving towards more dialogic systems in relation to their audience. The results reveal that NGOs have a high degree of interactivity in the tools used to present and disseminate information on their websites. However, not all maintain the same level of interactivity in the resources available for interaction with Internet users, as very few have high interactivity regarding bidirectional resources. It was concluded that international non-governmental organizations still suffer from certain shortcomings in the strategic management of digital communication on their web platforms, while, on the other hand, a strong presence can be noted on the most-popular social networks.
Research on Multi-Objective Optimization on Explosion-Suppression Structure-Nonmetallic Spherical Spacers
Authors: Liu Minjie , Yu Yangyang , Zhang Junhong , Wang Dan , Zhang Xueling , Yan Meng
Published: January 2024

Intense burning phenomena (fire disasters) need to be prevented in the combustible gas utilization and transportation processes to ensure industrial safety. Nonmetallic spherical spacers (NSSs) have been investigated and applied in lots of explosive atmospheres to prevent explosion execution in a confined space. In this work, a novel fuzzy-based analytic hierarchy process (FAHP) is developed to take into account the uncertainty in decision-making and effectively solve the problem of factor weight allocation in multi-objective optimization. Optimal Latin Hypercube Design (Opt LHD), Chebyshev Orthogonal Polynomials (COP), and Adaptive Simulated Annealing (ASA) were combined. A multi-objective optimization method is proposed for the structural parameter optimization problem on NSSs in order to achieve conflicting multiple-objective optimization of low displacement rate and minimal deformation. That is to say, the small volume (low displacement rate) and high explosion-suppression performance (minimal deformation) of NSSs were optimized simultaneously. The results show that, compared with the original NSS model’s deformation (2.85 mm) and displacement rate (3.63%), the optimized NSSs with weight allocation had optimized the deformation by 12.98% and displacement rate by 6.1%. Compared with the optimized design model of NSSs without weight allocation with a deformation of 2.75 mm and a displacement rate of 3.48%, the deformation has been optimized by 9.82%, and the displacement rate has been optimized by 2.0%. It was verified that the proposed method is effective. At the same time, it was verified that the suppression effect of NSSs can be enhanced by changing the shape of the NSS spacer reasonably by experimental verification.
Processing the Controllability of Control Systems with Distinct Fractional Derivatives via Kalman Filter and Gramian Matrix
Authors: Awadalla Muath , Chaouk Abir , Jneid Maher , Abuasbeh Kinda , Alahmadi Jihan
Published: January 2024

In this paper, we investigate the controllability conditions of linear control systems involving distinct local fractional derivatives. Sufficient conditions for controllability using Kalman rank conditions and the Gramian matrix are presented. We show that the controllability of the local fractional system can be determined by the invertibility of the Gramian matrix and the full rank of the Kalman matrix. We also show that the local fractional system involving distinct orders is controllable if and only if the Gramian matrix is invertible. Illustrative examples and an application are provided to demonstrate the validity of the theoretical findings.
Multivariate Multiscale Higuchi Fractal Dimension and Its Application to Mechanical Signals
Authors: Li Yuxing , Zhang Shuai , Liang Lili , Ding Qiyu
Published: January 2024

Fractal dimension, as a common nonlinear dynamics metric, is extensively applied in biomedicine, fault diagnosis, underwater acoustics, etc. However, traditional fractal dimension can only analyze the complexity of the time series given a single channel at a particular scale. To characterize the complexity of multichannel time series, multichannel information processing was introduced, and multivariate Higuchi fractal dimension (MvHFD) was proposed. To further analyze the complexity at multiple scales, multivariate multiscale Higuchi fractal dimension (MvmHFD) was proposed by introducing multiscale processing algorithms as a technology that not only improved the use of fractal dimension in the analysis of multichannel information, but also characterized the complexity of the time series at multiple scales in the studied time series data. The effectiveness and feasibility of MvHFD and MvmHFD were verified by simulated signal experiments and real signal experiments, in which the simulation experiments tested the stability, computational efficiency, and signal separation performance of MvHFD and MvmHFD, and the real signal experiments tested the effect of MvmHFD on the recognition of multi-channel mechanical signals. The experimental results show that compared to other indicators, A achieves a recognition rate of 100% for signals in three features, which is at least 17.2% higher than for other metrics.
PFHxS Exposure and the Risk of Non-Alcoholic Fatty Liver Disease
Authors: Ulhaq Zulvikar Syambani , Tse William Ka Fai
Published: January 2024

Perfluorohexanesulfonic acid (PFHxS) is a highly prevalent environmental pollutant, often considered to be less toxic than other poly- and perfluoroalkyl substances (PFASs). Despite its relatively lower environmental impact compared to other PFASs, several studies have suggested that exposure to PFHxS may be associated with disruptions of liver function in humans. Nevertheless, the precise pathomechanisms underlying PFHxS-induced non-alcoholic fatty liver disease (NAFLD) remain relatively unclear. Therefore, this study applied our previously published transcriptome dataset to explore the effects of PFHxS exposure on the susceptibility to NAFLD and to identify potential mechanisms responsible for PFHxS-induced NAFLD through transcriptomic analysis conducted on zebrafish embryos. Results showed that exposure to PFHxS markedly aggravated hepatic symptoms resembling NAFLD and other metabolic syndromes (MetS) in fish. Transcriptomic analysis unveiled 17 genes consistently observed in both NAFLD and insulin resistance (IR), along with an additional 28 genes identified in both the adipocytokine signaling pathway and IR. These shared genes were also found within the NAFLD dataset, suggesting that hepatic IR may play a prominent role in the development of PFHxS-induced NAFLD. In conclusion, our study suggests that environmental exposure to PFHxS could be a potential risk factor for the development of NAFLD, challenging the earlier notion of PFHxS being safer as previously claimed.
GIGANTEA Unveiled: Exploring Its Diverse Roles and Mechanisms
Authors: Liu Ling , Xie Yuxin , Yahaya Baba Salifu , Wu Fengkai
Published: January 2024

GIGANTEA (GI) is a conserved nuclear protein crucial for orchestrating the clock-associated feedback loop in the circadian system by integrating light input, modulating gating mechanisms, and regulating circadian clock resetting. It serves as a core component which transmits blue light signals for circadian rhythm resetting and overseeing floral initiation. Beyond circadian functions, GI influences various aspects of plant development (chlorophyll accumulation, hypocotyl elongation, stomatal opening, and anthocyanin metabolism). GI has also been implicated to play a pivotal role in response to stresses such as freezing, thermomorphogenic stresses, salinity, drought, and osmotic stresses. Positioned at the hub of complex genetic networks, GI interacts with hormonal signaling pathways like abscisic acid (ABA), gibberellin (GA), salicylic acid (SA), and brassinosteroids (BRs) at multiple regulatory levels. This intricate interplay enables GI to balance stress responses, promoting growth and flowering, and optimize plant productivity. This review delves into the multifaceted roles of GI, supported by genetic and molecular evidence, and recent insights into the dynamic interplay between flowering and stress responses, which enhance plants’ adaptability to environmental challenges.
The Burden of the Past: Globalized Crime, Trauma, and Patriarchal Violence in Horacio Castellanos Moya’s Moronga (2018)
Authors: González Calderón Julia
Published: January 2024

This article examines how trauma, crime, violence, and masculinity are connected in the novel Moronga (2018) by Honduran–Salvadoran author Horacio Castellanos Moya. The novel highlights the ways in which, thirty years after the signing of the Peace Accords, war trauma continues to oppress survivors of the civil war and determine their daily lives, beyond temporal and geographical borders. The novel points out how the transition into the neoliberal economy has transnationalized all aspects of the Salvadoran economy, including that of organized crime, which has undergone globalization, as have trauma and Salvadoran communities. Through the novel’s depiction of violence and crime, the author suggests that only those who perpetuate patriarchal violence in postwar diasporic communities will thrive, whereas those who aspire to carry out memory labor and peacefully heal the emotional wounds of the past will be defeated by the perverse logic of the system.
Reliability and Validation Study of Advance Care Planning Practice Scale among Health and Social Care Professionals
Authors: Che Sok-Leng , Leong Sok-Man , Lau Wing-Sze , Tam Kuai-In
Published: January 2024

Advance care planning (ACP) is a crucial process in clinical practice, enabling individuals to articulate their care preferences and goals, with significant implications for future healthcare. However, ACP practice of health and social care professionals (HSCPs) concerning patients, or their family members are rarely explored. The objective of the study was to adapt and validate a four-item scale assessing ACP practices of HSCPs toward patients or their family members. The ACP Practice Scale was evaluated through a cross-sectional online survey administered to HSCPs in Macao, assessing its factor structure, validity, and reliability. Based on a sample of 186 valid responses, the ACP Practice Scale demonstrated satisfactory levels of validity and reliability among HSPCs in Macao. The four-item scale explained 65.87% of the variance in ACP practice and exhibited strong internal consistency, with Cronbach’s alpha and McDonald’s omega coefficients of 0.82. Furthermore, item factor loadings ranged from 0.71 to 0.90. The ACP Practice Scale provides reliable and valid measurements of ACP practice among HSCPs. This instrument can help to enhance our understanding of ACP practices in clinical settings and support the advancement of advance care planning.
Garrè Sclerosing Osteomyelitis of the Clavicle: Clinical Results after Clavicular Resection
Authors: Calamita Andrea Gabriele , Stimolo Davide , Puccini Serena , Innocenti Matteo , Campanacci Domenico Andrea
Published: January 2024

(1) Background: Chronic non-bacterial osteomyelitis (CNO), also known as sclerosing osteomyelitis of Garrè, is a rare inflammatory bone disease with a specific clinical picture, uncertain pathogenesis, and no consensus on an effective treatment. Most frequently affecting other long bones, CNO may rarely involve the clavicle. The aim of this study was to present the results of a series of patients affected by CNO of the clavicle treated with total and partial clavicula resection. In addition, a literature review of different types of treatment of CNO was performed. (2) Methods: We retrospectively reviewed three patients with Sclerosing Osteomyelitis of Garre’ of the clavicle treated with partial resection of the clavicle (one) and with total clavicular resection (two). (3) Results: Patients (two female and one male) were an average age of 35.7 years at the time of the operation. At the 4-year follow-up, the mean active ROM was: 143° forward flexion, 133° abduction, 42° external rotation with an internal rotation of two patients at the interscapular level and one patient at the lumbosacral junction. The mean ASES score was 92/100 (range 87–100). In the literature review, after screening the abstracts and full texts for eligibility, 34 studies met the inclusion criteria. Conclusions: Partial or total clavicular resection resulted an effective treatment of CNO of the clavicle. The procedure seems to be particularly indicated after the failure of more conservative treatments.
Long-Term Effects of Microfiltered Seawater and Resistance Training with Elastic Bands on Hepatic Parameters, Inflammation, Oxidative Stress, and Blood Pressure of Older Women: A 32-Week, Double-Blinded, Randomized, Placebo-Controlled Trial
Authors: Babiloni-Lopez Carlos , Gargallo Pedro , Juesas Alvaro , Gene-Morales Javier , Saez-Berlanga Angel , Jiménez-Martínez Pablo , Casaña Jose , Benitez-Martinez Josep C. , Sáez Guillermo T. , Fernández-Garrido Julio , Alix-Fages Carlos , Colado Juan C.
Published: January 2024

The bulk of research on microfiltered seawater (SW) is based on its short-term effects. However, the long-term physiological adaptations to combining SW and resistance training (RT) are unknown. This study aimed to analyse the impact of an RT program using elastic bands combined with SW intake on hepatic biomarkers, inflammation, oxidative stress, and blood pressure in post-menopausal women. Ninety-three women voluntarily participated (age: 70 ± 6.26 years; body mass index: 22.05 ± 3.20 kg/m2; Up-and-Go Test: 6.66 ± 1.01 s). RT consisted of six exercises (32 weeks, 2 days/week). Nonsignificant differences were reported for hepatic biomarkers except for a reduction in glutamic-pyruvic transaminase (GPT) in both RT groups (RT + SW: p = 0.003, ES = 0.51; RT + Placebo: p = 0.012, ES = 0.36). Concerning oxidative stress, vitamin D increased significantly in RT + SW (p = 0.008, ES = 0.25). Regarding inflammation, interleukin 6 significantly decreased (p = 0.003, ES = 0.69) in RT + SW. Finally, systolic blood pressure significantly decreased in both RT groups (RT + placebo: p < 0.001, ES = 0.79; RT + SW: p < 0.001, ES = 0.71) as did diastolic blood pressure in both SW groups (RT + SW: p = 0.002, ES = 0.51; CON + SW: p = 0.028, ES = 0.50). Therefore, RT + SW or SW alone are safe strategies in the long term with no influences on hepatic and oxidative stress biomarkers. Additionally, SW in combination with RT positively influences vitamin D levels, inflammation, and blood pressure in older women.
Unlicensed/Off-Label Drug Prescriptions at Hospital Discharge in Children: An Observational Study Using Routinely Collected Health Data
Authors: Jaberi Elham , Boussaha Inesse , Dode Xavier , Grenet Guillaume , Kassai Behrouz , Nguyen Kim An
Published: January 2024

Background: Unlicensed and off-label (UL/OL) prescriptions have been associated with an increased risk of drug-related problems. Data of their prevalence at hospital discharge remain insufficient. We aimed to describe the prevalence of UL/OL drugs in outpatient prescriptions at discharge in children. Methods: We conducted a retrospective study using the routinely collected health data of children at discharge from 2014 to 2016. The primary reference source for determining licensed labelling was the summaries of product characteristics (SPCs) in a French industry-independent formulary named Thériaque. We described the characteristics of UL/OL prescriptions at discharge and looked for predictors of UL/OL prescriptions. Results: We included 2536 prescriptions of 479 children. Licensed, OL, and UL prescriptions accounted for 58.6% (95% CI: 56.7–60.5), 39.2% (95% CI: 37.3–41.1), and 2.3% (95% CI: 1.7–2.9), respectively. A total of 323 (74%) children received at least one UL/OL drug. Among the licensed drugs, bronchodilators (8.8%) and analgesics (8.6%), and among the OL drugs, antibiotics (2.8%), were the most prescribed. The younger age of the children and higher number of drugs they received increased the probability of UL/OL prescriptions (unadjusted p-value of ≤0.05). Conclusion: The prevalence of UL/OL prescriptions is about 40% at discharge from a pediatric university hospital in France.
Correction: Kanda et al. Body Compression Corrective Garment and Eating Behavioural Change for Weight Reduction: The Mutsu City Randomised Controlled Trial. Healthcare 2023, 11, 942
Authors: Kanda Akira , Sugimura Yoshikuni , Ohishi Hideki , Tatebayashi Satoru , Sawada Kaori , Wai Kyi Mar , Nishiguchi Kei , Tanabu Asano , Jung Songee , Murashita Koichi , Nakaji Shigeyuki , Ihara Kazushige
Published: January 2024

In the original publication [...]
Comparative Analysis between Virtual Visits and Pedagogical Outings to Heritage Sites: An Application in the Teaching of History
Authors: Corrales Mario , Rodríguez Fernando , Merchán María José , Merchán Pilar , Pérez Emiliano
Published: January 2024

In recent years, the teaching and learning of history have been transformed by the incorporation of active methodologies and digital tools. Virtual reality (VR) is one such tool that is being increasingly used in classrooms. It allows students to visit and experience spaces from other historical periods, even those that have been lost. However, due to its relatively slow adoption in formal educational settings, there is still a paucity of scientific references where this type of experience has been empirically tested to prove its potential benefits. Further research on this issue is therefore needed. This study presents a comparative analysis of the didactic use of historical heritage from the Roman period using two different methodological strategies: an educational visit to a museum and a virtual tour via electronic devices. The main objective is to compare the effectiveness of these two methods in terms of usability, learning outcomes, and emotional responses. The study was conducted with 51 primary school teachers in training. Data were collected using two ad hoc questionnaires, which were based on a previous one. A hybrid analysis methodology was adopted, with SPSS used for interpreting the quantitative data and Atlas Ti for the qualitative information. The results indicate that the two methodologies were perceived by future teachers as valuable resources for learning and teaching history. The emotions they evoked were primarily curiosity, joy, and motivation, with only minor occurrences of frustration, nervousness, or fear. The experience gained from this first application will make it possible to extend the study to other educational levels, to enrich the conclusions obtained and to evaluate the potential scope of using this combination of methods to develop historical competences and improve heritage education.
Evaluation of the Impact of Oral Health on the Daily Activities of Users of the National Health System
Authors: Wood Adriane , Pereira Antonio , Araújo Enoque , Ferigatto Júlia , Buexm Luisa , Barroso Eliane , Vazquez Fabiana
Published: January 2024

Background: the integration of dentistry services in the Unified Health System in Brazil (SUS) is essential in primary care assistance. Objective: we aimed to develop a tool for improving demand flowby evaluating the impact of oral health on the daily activities of users of the Family Health Unitusing the Oral Impacts of Daily Performance (OIDP)tool. Methods: In Barretos, Brazil, a cross-sectional study was conducted at a Family Health Unit (FHU)including patients over 12 years old. Oral health impact was assessed using the Oral Impacts of Daily Performance (OIDP) tool, and family risk was measured with the Coelho–Savassi scale. Results: 430 participants, including 411 adults and 19 young people, were recruited. Of the adults, 31% had an average OIDP score of 16.61. For young people, 53% reported an impact (average OIDP score: 28.61). Family risk (R1) was prevalent in 57.9% of young people and 53.3% of adults. Among adults, different activities were affected by risk: smiling without embarrassment (risk level 2), enjoying contact with people (risk level 3), and performing one’s job or social role (risk level 1). Emotional state (R3) had the lowest OIDP score (p = 0.029). Conclusion: implementation of the OIDP scale in clinical practice enhances healthcare planning and ensures better-quality and equitable services, thus emphasizing comprehensive oral healthcare within the SUS.
Clinical Impact of “Real World Data” and Blockchain on Public Health: A Scoping Review
Authors: Milone Virginia , Fusco Antonio , De Feo Angelamaria , Tatullo Marco
Published: January 2024

The digitisation of healthcare has allowed a significant rethinking of the previous clinical protocols, improving their interoperability through substantial standardisation. These technological advances have ensured that data are comparable, as they are obtained from ‘reliable’ and certified processes; however, there are billions of data that are neither structured nor quality-controlled. They are collectively referred to as ‘Real World Data’ (RWD). Blockchain (BC) is a procedure with specific characteristics and algorithms that ensure that the stored data cannot be tampered with. Nowadays, there is an increasing need to rethink blockchain in a one-health vision, making it more than just a ‘repository’ of data and information, but rather an active player in the process of data sharing. In this landscape, several scholars have analysed the potential benefits of BC in healthcare, focusing on the sharing and safety of clinical data and its contact tracing applications. There is limited research on this matter; moreover, there is a strategic interest in managing RWD in a reliable and comparable way, despite the lack of knowledge on this topic. Our work aims to analyse systematically the most impacting literature, highlighting the main aspects of BC within the context of the new digital healthcare, and speculating on the unexpressed potential of RWD.
Changes in Sexual Behavior and Satisfaction and Violent Behavior during COVID-19 Lockdown: Explorative Results from the Italian Cross-Sectional Study of the I-SHARE Multi-Country Project
Authors: Nimbi Filippo Maria , Cavagnis Sara , Eleuteri Stefano
Published: January 2024

Background: The COVID-19 pandemic has had effects on sexual and reproductive health and intimate partner violence (IPV). This study aims to describe changes in sexual health and IPV in the Italian population both during and after the lockdowns. Methods: This cross-sectional online study was conducted, as part of the I-SHARE multicountry project, between June 2020 and January 2021. Recruitment was carried out through convenience sampling; a total of 329 participants were included in the analysis. A generalized linear model was used to analyze the difference in sexual health and IPV variables before, during, and after the lockdown. Results: Fifty-three percent of the participants reported having sexual problems during the first wave of the pandemic. Sexual satisfaction decreased during the first wave, and then, returned to the pre-lockdown level. While during the lockdown, some activities were reduced (such as kissing, cuddling, and sexual activities with a steady partner), for other activities, no difference was reported (such as masturbation, sexual activities with casual partners, or sexting). Few participants reported having issues accessing HIV testing and contraception. There was no difference in terms of physical and sexual violence, while there was a significant decrease in feeling vulnerable to sexual or physical assault during the first wave. Conclusions: The first wave of the pandemic has had significant effects on sexual health. This should be taken into consideration when preparing for future epidemics and health emergencies.
HvGSK1.1 Controls Salt Tolerance and Yield through the Brassinosteroid Signaling Pathway in Barley
Authors: Kloc Yuliya , Dmochowska-Boguta Marta , Żebrowska-Różańska Paulina , Łaczmański Łukasz , Nadolska-Orczyk Anna , Orczyk Wacław
Published: January 2024

Brassinosteroids (BRs) are a class of plant steroid hormones that are essential for plant growth and development. BRs control important agronomic traits and responses to abiotic stresses. Through the signaling pathway, BRs control the expression of thousands of genes, resulting in a variety of biological responses. The key effectors of the BR pathway are two transcription factors (TFs): BRASSINAZOLE RESISTANT 1 (BZR1) and BRI1-EMSSUPPRESSOR 1 (BES1). Both TFs are phosphorylated and inactivated by the Glycogen synthase kinase 3 BRASSINOSTEROID INSENSITIVE2 (BIN2), which acts as a negative regulator of the BR pathway. In our study, we describe the functional characteristics of HvGSK1.1, which is one of the GSK3/SHAGGY-like orthologs in barley. We generated mutant lines of HvGSK1.1 using CRISPR/Cas9 genome editing technology. Next Generation Sequencing (NGS) of the edited region of the HvGSK1.1 showed a wide variety of mutations. Most of the changes (frameshift, premature stop codon, and translation termination) resulted in the knock-out of the target gene. The molecular and phenotypic characteristics of the mutant lines showed that the knock-out mutation of HvGSK1.1 improved plant growth performance under salt stress conditions and increased the thousand kernel weight of the plants grown under normal conditions. The inactivation of HvGSK1.1 enhanced BR-dependent signaling, as indicated by the results of the leaf inclination assay in the edited lines. The plant traits under investigation are consistent with those known to be regulated by BRs. These results, together with studies of other GSK3 gene members in other plant species, suggest that targeted editing of these genes may be useful in creating plants with improved agricultural traits.
Navigating the Cytokine Seas: Targeting Cytokine Signaling Pathways in Cancer Therapy
Authors: Stanilov Noyko , Velikova Tsvetelina , Stanilova Spaska
Published: January 2024

Cancer remains one of the leading causes of morbidity and mortality worldwide, necessitating continuous efforts to develop effective therapeutic strategies. Over the years, advancements in our understanding of the complex interplay between the immune system and cancer cells have led to the development of immunotherapies that revolutionize cancer treatment. Cytokines, as key regulators of the immune response, are involved in both the initiation and progression of cancer by affecting inflammation and manipulating multiple intracellular signaling pathways that regulate cell growth, proliferation, and migration. Cytokines, as key regulators of inflammation, have emerged as promising candidates for cancer therapy. This review article aims to provide an overview of the significance of cytokines in cancer development and therapy by highlighting the importance of targeting cytokine signaling pathways as a potential therapeutic approach.
Aluminium-Based Metal–Organic Framework Nano Cuboids and Nanoflakes with Embedded Gold Nanoparticles for Carbon Dioxide Fixation with Epoxides into Cyclic Esters
Authors: Kopacka Gabriela , Wasiluk Kinga , Majewski Pawel W. , Kopyt Michał , Kwiatkowski Piotr , Megiel Elżbieta
Published: January 2024

The fixation of carbon dioxide with epoxides is one of the most attractive methods for the green utilisation of this greenhouse gas and leads to many valuable chemicals. This process is characterised by 100% atom efficiency; however, an efficient catalyst is required to achieve satisfactory yields. Metal–organic frameworks (MOFs) are recognised as being extremely promising for this purpose. Nevertheless, many of the proposed catalysts are based on ions of rare elements or elements not entirely safe for the environment; this is notable with commercially unavailable ligands. In an effort to develop novel catalysts for CO2 fixation on an industrial scale, we propose novel MOFs, which consist of aluminium ions coordinated with commercially available 1,4-naphthalene dicarboxylic acid (Al@NDC) and their nanocomposites with gold nanoparticles entrapped inside their structure (AlAu@NDC). Due to the application of 4-amino triazole and 5-amino tetrazole as crystallization mediators, the morphology of the synthesised materials can be modified. The introduction of gold nanoparticles (AuNPs) into the structure of the synthesised Al-based MOFs causes the change in morphology from nano cuboids to nanoflakes, simultaneously decreasing their porosity. However, the homogeneity of the nanostructures in the system is preserved. All synthesised MOF materials are highly crystalline, and the simulation of PXRD patterns suggests the same tetragonal crystallographic system for all fabricated nanomaterials. The fabricated materials are proven to be highly efficient catalysts for carbon dioxide cycloaddition with a series of model epoxides: epichlorohydrin; glycidol; styrene oxide; and propylene oxide. Applying the synthesised catalysts enables the reactions to be performed under mild conditions (90 °C; 1 MPa CO2) within a short time and with high conversion and yield (90% conversion of glycidol towards glycerol carbonate with 89% product yield within 2 h). The developed nanocatalysts can be easily separated from the reaction mixture and reused several times (both conversion and yield do not change after five cycles). The excellent performance of the fabricated catalytic materials might be explained by their high microporosity (from 421 m2 g−1 to 735 m2 g−1); many catalytic centres in the structure exhibit Lewis acids’ behaviour, increased capacity for CO2 adsorption, and high stability. The presence of AuNPs in the synthesised nanocatalysts (0.8% w/w) enables the reaction to be performed with a higher yield within a shorter time; this is especially important for less-active epoxides such as propylene oxide (two times higher yield was obtained using a nanocomposite, in comparison with Al-MOF without nanoparticles).
In Vitro Analysis of the Antagonistic Biological and Chemical Interactions between the Endophyte Sordaria tomento-alba and the Phytopathogen Botrytis cinerea
Authors: Bolívar-Anillo Hernando José , Izquierdo-Bueno Inmaculada , González-Rey Estrella , González-Rodríguez Victoria E. , Cantoral Jesús M. , Collado Isidro G. , Garrido Carlos
Published: January 2024

Plant pathogenic infections causing substantial global food losses are a persistent challenge. This study investigates a potential biocontrol strategy against the necrotrophic fungus Botrytis cinerea using the endophytic fungus Sordaria tomento-alba isolated from Gliricidia sepium in Colombia. Today, synthetic fungicides dominate B. cinerea control, raising environmental and health concerns. S. tomento-alba exhibits notable in vitro effects, inhibiting B. cinerea growth by approximately 60% during co-culture and 50% in double disc co-culture. Additionally, it suppresses botryanes production and produces the compound heptacyclosordariolone, which has proven effective in inhibiting B. cinerea mycelial growth and spore germination in vitro. This biocontrol agent could be a potential eco-friendly alternative to replace synthetic fungicides. Our study provides insights into the chemical and biological mechanisms underpinning the antagonistic activity of S. tomento-alba, emphasizing the need for further research to understand its biosynthesis pathways and optimize its biocontrol potential. It also contributes molecular evidence of fungal interactions with implications for advanced forums in molecular studies in biology and chemistry, particularly in addressing plant pathogenic infections and promoting sustainable agriculture.
A Novel Fabrication of Heterogeneous Saponified Poly(Vinyl Alcohol)/Pullulan Blend Film for Improved Wound Healing Application
Authors: Yeasmin Sabina , Jung Jae Hoon , Lee Jungeon , Kim Tae Young , Yang Seong Baek , Kwon Dong-Jun , Kim Myoung Ok , Yeum Jeong Hyun
Published: January 2024

In this study, a novel film of poly(vinyl alcohol) (PVA)/pullulan (PULL) with improved surface characteristics was prepared from poly(vinyl acetate) (PVAc)/PULL blend films with various mass ratios after the saponification treatment in a heterogeneous medium. According to proton nuclear magnetic resonance (1H-NMR), Fourier transform infrared, and X-ray diffraction results, it was established that the successful fabrication of saponified PVA/PULL (100/0, 90/10, and 80/20) films could be obtained from PVAc/PULL (100/0, 90/10, and 80/20) films, respectively, after 72 h saponification at 50 °C. The degree of saponification calculated from 1H-NMR analysis results showed that fully saponified PVA was obtained from all studied films. Improved hydrophilic characteristics of the saponified films were revealed by a water contact angle test. Moreover, the saponified films showed improved mechanical behavior, and the micrographs of saponified films showed higher surface roughness than the unsaponified films. This kind of saponified film can be widely used for biomedical applications. Moreover, the reported saponified film dressing extended the lifespan of dressing as determined by its self-healing capacity and considerably advanced in vivo wound-healing development, which was attributed to its multifunctional characteristics, meaning that saponified film dressings are promising candidates for full-thickness skin wound healing.
Generation and Application of Monoclonal Antibodies against Porcine S100A8, S100A9, and S100A12 Proteins Using Hybridoma Technology
Authors: Xia Pengpeng , Ma Xin , Yan Li , Lian Siqi , Li Xiangyu , Luo Yi , Chen Ziyue , Ji Xingduo
Published: January 2024

S100A8, S100A9, and S100A12 proteins are important members of the S100 protein family, act primarily as congenital immunomodulators, and are closely related to the occurrence of infectious diseases. There have been few reports on the functional properties of S100A8, S100A9, and S100A12 proteins in swine, but it is certain that porcine S100A8, S100A9, and S100A12 proteins are highly expressed in diseased swine. To address the current lack of reliable and timely detection tools for these three proteins, we generated monoclonal antibodies specific to the porcine S100A8, S100A9, and S100A12 proteins using hybridoma technology. The results of serum sample testing showed that the above monoclonal antibodies specifically recognize the proteins S100A8, S100A9, and S100A12 in the serum and were able to evaluate the content change of these proteins during the infection process. This provides the basis for the use of porcine S100A8, S100A9, and S100A12 in the surveillance and diagnosis of swine diseases and laid a foundation for further understanding their roles in infection, immunity, and inflammation, as well as their potential applications in preventing or treating gastrointestinal tract or inflammatory diseases in swine.