bluuebunny/crossref_metadata_2025 huggingface dataset:
Development of Fluorescently Labeled SSEA-3, SSEA-4, and Globo-H Glycosphingolipids for Elucidating Molecular Interactions in the Cell Membrane
Authors: Asano Sachi , Pal Rita , Tanaka Hide-Nori , Imamura Akihiro , Ishida Hideharu , Suzuki Kenichi G. N. , Ando Hiromune
Published: December 2019

Glycosphingolipids (GSLs), such as the globo-series GSLs stage-specific embryonic antigen 3 (SSEA-3), SSEA-4, and Globo-H, are specifically expressed on pluripotent stem cells and cancer cells, and are known to be associated with various biological processes such as cell recognition, cell adhesion, and signal transduction. However, the behavior and biological roles of these GSLs are still unclear. In our previous study, we observed the interactions between the lipid raft and GSLs in real-time using single-molecule imaging, where we successfully synthesized various fluorescent analogs of GSLs (e.g., GM1 and GM3). Here, we have developed fluorescent analogs of SSEA-3, SSEA-4, and Globo-H using chemical synthesis. The biophysical properties of these analogs as raft markers were examined by partitioning giant plasma membrane vesicles from RBL-2H3 cells into detergent-resistant membrane fractions and liquid-ordered/liquid-disordered phases. The results indicated that the analogs were equivalent to native-type GSLs. The analogs could be used to observe the behavior of globo-series GSLs for detailing the structure and biological roles of lipid rafts and GSL-enriched nanodomains during cell differentiation and cell malignancy.
Synaptic Plasticity Shapes Brain Connectivity: Implications for Network Topology
Authors: Stampanoni Bassi Mario , Iezzi Ennio , Gilio Luana , Centonze Diego , Buttari Fabio
Published: December 2019

Studies of brain network connectivity improved understanding on brain changes and adaptation in response to different pathologies. Synaptic plasticity, the ability of neurons to modify their connections, is involved in brain network remodeling following different types of brain damage (e.g., vascular, neurodegenerative, inflammatory). Although synaptic plasticity mechanisms have been extensively elucidated, how neural plasticity can shape network organization is far from being completely understood. Similarities existing between synaptic plasticity and principles governing brain network organization could be helpful to define brain network properties and reorganization profiles after damage. In this review, we discuss how different forms of synaptic plasticity, including homeostatic and anti-homeostatic mechanisms, could be directly involved in generating specific brain network characteristics. We propose that long-term potentiation could represent the neurophysiological basis for the formation of highly connected nodes (hubs). Conversely, homeostatic plasticity may contribute to stabilize network activity preventing poor and excessive connectivity in the peripheral nodes. In addition, synaptic plasticity dysfunction may drive brain network disruption in neuropsychiatric conditions such as Alzheimer’s disease and schizophrenia. Optimal network architecture, characterized by efficient information processing and resilience, and reorganization after damage strictly depend on the balance between these forms of plasticity.
Mutation of Aspartate 238 in FAD Synthase Isoform 6 Increases the Specific Activity by Weakening the FAD Binding
Authors: Leone Piero , Galluccio Michele , Quarta Stefano , Anoz-Carbonell Ernesto , Medina Milagros , Indiveri Cesare , Barile Maria
Published: December 2019

FAD synthase (FADS, or FMN:ATP adenylyl transferase) coded by the FLAD1 gene is the last enzyme in the pathway of FAD synthesis. The mitochondrial isoform 1 and the cytosolic isoform 2 are characterized by the following two domains: the C-terminal PAPS domain (FADSy) performing FAD synthesis and pyrophosphorolysis; the N-terminal molybdopterin-binding domain (FADHy) performing a Co++/K+-dependent FAD hydrolysis. Mutations in FLAD1 gene are responsible for riboflavin responsive and non-responsive multiple acyl-CoA dehydrogenases and combined respiratory chain deficiency. In patients harboring frameshift mutations, a shorter isoform (hFADS6) containing the sole FADSy domain is produced representing an emergency protein. With the aim to ameliorate its function we planned to obtain an engineered more efficient hFADS6. Thus, the D238A mutant, resembling the D181A FMNAT “supermutant” of C. glabrata, was overproduced and purified. Kinetic analysis of this enzyme highlighted a general increase of Km, while the kcat was two-fold higher than that of WT. The data suggest that the FAD synthesis rate can be increased. Additional modifications could be performed to further improve the synthesis of FAD. These results correlate with previous data produced in our laboratory, and point towards the following proposals (i) FAD release is the rate limiting step of the catalytic cycle and (ii) ATP and FMN binding sites are synergistically connected.
Art in the Age of Social Media: Interaction Behavior Analysis of Instagram Art Accounts
Authors: Kang Xin , Chen Wenyin , Kang Jian
Published: December 2019

Instagram is the top preferred social media platform in the art world, however, we know little about the features of the most-liked artworks, and what role does the interaction between artists and followers play in the most-liked artworks? This study used quantitative and qualitative methods to examine the interaction between artists and followers on Instagram and the features of the most-liked artworks. Data from 706 artists’ accounts 497,649 posts on Instagram and 35 questionnaires. The results reveal that likes and comments were greatly influenced by interactions, with confusion and curiosity being a big reason to engage. The artist’s life experience and interaction with the followers had a positive influence on the most-liked artworks. Interaction with followers does not have much impact on their artistic creation, although artists expect more likes. Our study expands the research of mobile social media interaction in the art world, which is of great significance for the research on the interactive psychology of artwork and digital marketing communications on social media. The findings can also support future research on citizen curators and sociology analytics research areas.
An Improved Ship Collision Risk Evaluation Method for Korea Maritime Safety Audit Considering Traffic Flow Characteristics
Authors: Yoo Yunja , Kim Tae-Goun
Published: December 2019

Ship collision accidents account for the majority of marine accidents. The collision risk can be even greater in ports where the traffic density is high and terrain conditions are difficult. The proximity assessment model of the Korea Maritime Safety Audit (KMSA), which is a tool for improving maritime traffic safety, employs a normal distribution of ship traffic to calculate the ship collision risk. However, ship traffic characteristics can differ according to the characteristics of the sea area and shipping route. Therefore, this study simulates collision probabilities by estimating the best-fit distribution function of ship traffic flow in Ulsan Port, which is the largest hazardous cargo vessel handling port in Korea. A comparison of collision probability simulation results using the best-fit function and the normal distribution function reveals a difference of approximately 1.5–2.4 times for each route. Moreover, the collision probability estimates are not accurate when the normal distribution function is uniformly applied without considering the characteristics of each route. These findings can be used to improve the KMSA evaluation method for ship collision risks, particularly in hazardous port areas.
The Use of Community Greenways: A Case Study on A Linear Greenway Space in High Dense Residential Areas, Guangzhou
Authors: Chi Wenxiu , Lin Guangsi
Published: December 2019

The community greenway is a kind of greenway that goes through high-density residential areas in the city and is closely related to residents’ life. However, few scholars focus on how this type of greenways serves the everyday life of the community as an integrated resource. This aspect is important because the everyday life in the public space involves multiple activities. How to coordinate and satisfy these activities relates to the benefits of community greenways. Therefore, this paper takes a representative community greenway in Haizhu District of Guangzhou as an example, to study whether community greenways match the needs of necessary activities, optional activities and social activities. The usage patterns, the evaluation of the current status, the impact on everyday activities, and the importance of different construction factors were surveyed. The applied methods include site observation, questionnaires and interviews. The results show that more than 90% of users are from communities within 1 mile from the community greenway. More than half of the users (55%) are satisfied with the community greenways. Furthermore, the community greenways benefit the everyday activities of residents, such as transportation, recreation, social interaction and also other minor but important everyday activities. However, from the perspective of residents’ requirements for construction factors, the status of service facilities needs to be improved. The characteristics, overall benefits, and construction implications of community greenways are therefore discussed. Community greenways can be important open space for residents and this paper is significant on community greenways meeting the needs of residents’ everyday activities, thus, to provide a better community living environment and to build a better urban open space system.
Feminist Interventions in Law Reform: Criminalising Image-Based Sexual Abuse in New South Wales
Authors: McLoughlin Kcasey , O’Brien Alex
Published: December 2019

Feminist legal theorists have had something of an uneasy relationship with law reform. Although feminist academics and lawyers have contributed much to law reform efforts that have sought to improve women’s lives, feminists have nonetheless taken divergent positions regarding the extent to which these efforts can truly dismantle the masculinist character of law through law reform projects. This article revisits these tensions and, in so doing, seeks to better understand the extent to which feminists can meaningfully contribute to law reform projects. The criminalisation of image-based sexual abuse in New South Wales (Australia) serves as a case study to examine and re-examine these tensions. In September 2016, the New South Wales government announced that it was proposing to criminalise the distribution of certain images without consent. Following a public consultation process, the government legislated for a new offense directed at the distribution of these images. Although there is certainly not one all-encompassing feminist understanding of image-based sexual abuse, the importance of understanding this practice as abuse and as existing within a culture that normalises and sustains nonconsensual activity nonetheless has been a key feminist concern in agitating for law reform in this area. This article examines the extent to which the legislative response took seriously the harms engendered by image-based sexual abuse.
Light Transmittance and Polymerization of Bulk-Fill Composite Materials Doped with Bioactive Micro-Fillers
Authors: Dieckmann Phoebe , Mohn Dirk , Zehnder Matthias , Attin Thomas , Tauböck Tobias T.
Published: December 2019

This study investigated the effect of bioactive micro-fillers on the light transmittance and polymerization of three commercially available bulk-fill resin composites. These were mixed with 20 wt% bioactive glass 45S5, Portland cement, inert dental barium glass, or nothing (controls). Composites were photo-activated and light transmittance through 4 mm thick specimens was measured in real time. Moreover, degree of conversion (DC) and Knoop hardness (KHN) were assessed. Light transmittance of all bulk-fill composites significantly decreased (p < 0.05) with addition of 20 wt% bioactive glass 45S5 but not when inert barium glass was added. For bulk-fill composites modified with Portland cement, light irradiance dropped below the detection limit at 4 mm depth. The DC at the top surface of the specimens was not affected by addition of bioactive or inert micro-fillers. The bottom-to-top ratio of both DC and KHN surpassed 80% for bulk-fill composites modified with 20 wt% bioactive or inert glass fillers but fell below 20% when the composites were modified with Portland cement. In contrast to Portland cement, the addition of 20 wt% bioactive glass maintains adequate polymerization of bulk-fill composites placed at 4 mm thickness, despite a decrease in light transmittance compared to the unmodified materials.
Thermal Reactivity in Metal Organic Materials (MOMs): From Single-Crystal-to-Single-Crystal Reactions and Beyond
Authors: Martí-Rujas Javier
Published: December 2019

Thermal treatment is important in the solid-state chemistry of metal organic materials (MOMs) because it can create unexpected new structures with unique properties and applications that otherwise in the solution state are very difficult or impossible to achieve. Additionally, high-temperature solid-state reactivity provide insights to better understand chemical processes taking place in the solid-state. This review article describes relevant thermally induced solid-state reactions in metal organic materials, which include metal organic frameworks (MOFs)/coordination polymers (CPs), and second coordination sphere adducts (SSCs). High temperature solid-state reactivity can occur in a single-crystal-to-single crystal manner (SCSC) usually for cases where there is small atomic motion, allowing full structural characterization by single crystal X-ray diffraction (SC-XRD) analysis. However, for the cases in which the structural transformations are severe, often the crystallinity of the metal-organic material is damaged, and this happens in a crystal-to-polycrystalline manner. For such cases, in the absence of suitable single crystals, structural characterization has to be carried out using ab initio powder X-ray diffraction analysis or pair distribution function (PDF) analysis when the product is amorphous. In this article, relevant thermally induced SCSC reactions and crystal-to-polycrystalline reactions in MOMs that involve significant structural transformations as a result of the molecular/atomic motion are described. Thermal reactivity focusing on cleavage and formation of coordination and covalent bonds, crystalline-to-amorphous-to-crystalline transformations, host–guest behavior and dehydrochlorination reactions in MOFs and SSCs will be discussed.
An Experimental and Numerical Analysis of the Compression of Bimetallic Cylinders
Authors: Camacho Ana María , Rodríguez-Prieto Álvaro , Herrero José Manuel , Aragón Ana María , Bernal Claudio , Lorenzo-Martin Cinta , Yanguas-Gil Ángel , Martins Paulo A. F.
Published: December 2019

This paper investigates the upsetting of bimetallic cylinders with an aluminum alloy center and a brass ring. The influence of the center-ring shape factor and type of assembly fit (interference and clearance), and the effect of friction on the compression force and ductile damage are comprehensively analyzed by means of a combined numerical-experimental approach. Results showed that the higher the shape factor, the lower the forces required, whereas the effect of friction is especially important for cylinders with the lowest shape factors. The type of assembly fit does not influence the compression force. The accumulated ductile damage in the compression of bimetallic cylinders is higher than in single-material cylinders, and the higher the shape factor, the lower the damage for the same amount of stroke. The highest values of damaged were found to occur at the middle plane, and typically in the ring. Results also showed that an interference fit was more favorable for preventing fracture of the ring than a clearance fit. Microstructural analysis by scanning electron microscopy revealed a good agreement with the finite element predicted distribution of ductile damage.
Atom Probe Tomography Analysis of TiCx Powders Synthesized by SHS in Al/Fe/Cu–Ti–C Systems
Authors: Jin Shenbao , Su Haokai , Sha Gang
Published: December 2019

The stoichiometry of titanium carbide (TiCx) particles is important in determining particle properties. Spherical TiCx powders with particle sizes of 1–5 μm were produced by self-propagating high-temperature synthesis (SHS) in 30 wt.% Al–, 30 wt.% Cu–, and 30 wt.% Fe–Ti–C systems, respectively. To measure the compositions of the carbide powders, atom probe tomography (APT) tip specimens were carefully prepared by using a focus ion-beam milling method. APT analysis revealed that the TiCx particles formed in Al–, Cu–, and Fe–Ti–C systems are highly substoichiometric. The results are consistent with observations of the TiCx particles with a high content of oxygen and a certain amount of secondary metallic elements (Al, Cu, and Fe).
A Parasitic Resistance-Adapted Programming Scheme for Memristor Crossbar-Based Neuromorphic Computing Systems
Authors: Ngoc Truong Son
Published: December 2019

Memristor crossbar arrays without selector devices, such as complementary-metal oxide semiconductor (CMOS) devices, are a potential for realizing neuromorphic computing systems. However, wire resistance of metal wires is one of the factors that degrade the performance of memristor crossbar circuits. In this work, we propose a wire resistance modeling method and a parasitic resistance-adapted programming scheme to reduce the impact of wire resistance in a memristor crossbar-based neuromorphic computing system. The equivalent wire resistances for the cells are estimated by analyzing the crossbar circuit using the superposition theorem. For the conventional programming scheme, the connection matrix composed of the target memristance values is used for crossbar array programming. In the proposed parasitic resistance-adapted programming scheme, the connection matrix is updated before it is used for crossbar array programming to compensate the equivalent wire resistance. The updated connection matrix is obtained by subtracting the equivalent connection matrix from the original connection matrix. The circuit simulations are performed to test the proposed wire resistance modeling method and the parasitic resistance-adapted programming scheme. The simulation results showed that the discrepancy of the output voltages of the crossbar between the conventional wire resistance modeling method and the proposed wire resistance modeling method is as low as 2.9% when wire resistance varied from 0.5 to 3.0 Ω. The recognition rate of the memristor crossbar with the conventional programming scheme is 99%, 95%, 81%, and 65% when wire resistance is set to be 1.5, 2.0, 2.5, and 3.0 Ω, respectively. By contrast, the memristor crossbar with the proposed parasitic resistance-adapted programming scheme can maintain the recognition as high as 100% when wire resistance is as high as 3.0 Ω.
312 MAX Phases: Elastic Properties and Lithiation
Authors: Filippatos P.P. , Hadi M.A. , Christopoulos S.-R.G. , Kordatos A. , Kelaidis N. , Fitzpatrick M.E. , Vasilopoulou M. , Chroneos A.
Published: December 2019

Interest in the Mn+1AXn phases (M = early transition metal; A = group 13–16 elements, and X = C or N) is driven by their ceramic and metallic properties, which make them attractive candidates for numerous applications. In the present study, we use the density functional theory to calculate the elastic properties and the incorporation of lithium atoms in the 312 MAX phases. It is shown that the energy to incorporate one Li atom in Mo3SiC2, Hf3AlC2, Zr3AlC2, and Zr3SiC2 is particularly low, and thus, theoretically, these materials should be considered for battery applications.
Parametric Analysis of the Mandrel Geometrical Data in a Cold Expansion Process of Small Holes Drilled in Thick Plates
Authors: Calaf-Chica Jose , Marín Marta María , Rubio Eva María , Teti Roberto , Segreto Tiziana
Published: December 2019

Cold expansion technology is a cold-forming process widely used in aeronautics to extend the fatigue life of riveted and bolted holes. During this process, an oversized mandrel is pushed through the hole in order to yield it and generate compressive residual stresses contributing to the fatigue life extension of the hole. In this paper, a parametric analysis of the mandrel geometrical data (inlet angle straight zone length and diametric interference) and their influence on the residual stresses was carried out using a finite element method (FEM). The obtained results were compared with the conclusions presented in a previous parametric FEM analysis on the influence of the swage geometry in a swaging cold-forming process of gun barrels. This process could be considered, in a simplified way, as a scale-up of the cold expansion process of small holes, and this investigation demonstrated the influence of the diameter ratio (K) on the relation between the mandrel or swage geometry and the residual stresses obtained after the cold-forming process.
Compressive Behaviour of Closed-Cell Aluminium Foam at Different Strain Rates
Authors: Novak Nejc , Vesenjak Matej , Duarte Isabel , Tanaka Shigeru , Hokamoto Kazuyuki , Krstulović-Opara Lovre , Guo Baoqiao , Chen Pengwan , Ren Zoran
Published: December 2019

Closed-cell aluminium foams were fabricated and characterised at different strain rates. Quasi-static and high strain rate experimental compression testing was performed using a universal servo-hydraulic testing machine and powder gun. The experimental results show a large influence of strain rate hardening on mechanical properties, which contributes to significant quasi-linear enhancement of energy absorption capabilities at high strain rates. The results of experimental testing were further used for the determination of critical deformation velocities and validation of the proposed computational model. A simple computational model with homogenised crushable foam material model shows good correlation between the experimental and computational results at analysed strain rates. The computational model offers efficient (simple, fast and accurate) analysis of high strain rate deformation behaviour of a closed-cell aluminium foam at different loading velocities.
The Characterization of Stress Corrosion Cracking in the AE44 Magnesium Casting Alloy Using Quantitative Fractography Methods
Authors: Sozańska Maria , Mościcki Adrian , Czujko Tomasz
Published: December 2019

In this work an assessment of the susceptibility of the AE44 magnesium alloy to stress corrosion cracking in a 0.1M Na2SO4 environment is presented. The basic assumed criterion for assessing the alloy behavior under complex mechanical and corrosive loads is deterioration in mechanical properties (elongation, reduction in area, tensile strength and time to failure). The AE44 magnesium alloy was subjected to the slow strain rate test (SSR) in air and in a corrosive environment under open circuit potential (OCP) conditions. In each variant, the content of hydrogen in the alloy was determined. The obtained fractures were subjected to a quantitative evaluation by original fractography methods. It was found that under stress corrosion cracking (SCC) conditions and in the presence of hydrogen the mechanical properties of AE44 deteriorated. The change in the mechanical properties under SCC conditions in a corrosive environment was accompanied by the presence of numerous cracks, both on fracture surfaces and in the alloy microstructure. The developed method for the quantitative evaluation of cracks on the fracture surface turned out to be a more sensitive method, enabling the assessment of the susceptibility of AE44 under complex mechanical and corrosive loads in comparison with deterioration in mechanical properties. Mechanical tests showed a decrease in properties after SSRT tests in corrosive environments (UTS ≈ 153 MPa, ε = 11.2%, Z = 4.0%) compared to the properties after air tests (UTS ≈ 166 MPa, ε = 11.9%, Z = 7.8%) but it was not as visible as the results of quantitative assessment of cracks at fractures (number of cracks, length of cracks): after tests in corrosive environment (900; 21.3 μm), after tests in air (141; 34.5 μm). These results indicate that the proposed new proprietary test methodology can be used to quantify the SSC phenomenon in cases of slight changes in mechanical properties after SSRT tests in a corrosive environment in relation to the test results in air.
Multinuclear Magnetic Resonance Study of Sodium Salts in Water Solutions
Authors: Makulski Włodzimierz
Published: December 2019

The small amounts of gaseous 3He dissolved in low concentrated water solutions of NaCl, NaNO3 and NaClO4 were prepared and examined by 3He-, 23Na-, 35Cl- and 15N-NMR spectroscopy. This experimental data, along with new theoretical shielding factors, was used to measure the 23Na nuclear magnetic moment against that of helium-3 μ(23Na) = +2.2174997(111) in nuclear magnetons. The standard relationship between NMR frequencies and nuclear magnetic moments of observed nuclei was used. The nuclear magnetic shielding factors of 23Na cation were verified against that of counter ions present in water solutions. Very good agreement between shielding constants σ(3He), σ(23Na+), σ(35Cl‒), σ(35ClO4‒), σ(15NO3‒) in water at infinite dilution and nuclear magnetic moments was observed for all magnetic nuclei. It can be used as a reference nucleus for calculating a few other magnetic moments of different nuclei by the NMR method. An analysis of new and former μ(23Na) experimental data obtained by the atomic beam magnetic resonance method (ABMR) and other NMR measurements shows good replicability of all specified results. The composition of sodium water complexes was discussed in terms of chemical equilibria and NMR shielding scale.
Some Characterizations of Semi-Invariant Submanifolds of Golden Riemannian Manifolds
Authors: Gök Mustafa , Keleş Sadık , Kılıç Erol
Published: December 2019

In this paper, we study some characterizations for any submanifold of a golden Riemannian manifold to be semi-invariant in terms of canonical structures on the submanifold, induced by the golden structure of the ambient manifold. Besides, we determine forms of the distributions involved in the characterizations of a semi-invariant submanifold on both its tangent and normal bundles.
Niobium Additions to a 15%Cr–3%C White Iron and Its Effects on the Microstructure and on Abrasive Wear Behavior
Authors: Bedolla-Jacuinde Arnoldo , Guerra Francisco , Mejia Ignacio , Vera Uzzi
Published: December 2019

From the present study, niobium additions of 1.79% and 3.98% were added to a 15% Cr–3% C white iron, and their effects on the microstructure, hardness and abrasive wear were analyzed. The experimental irons were melted in an open induction furnace and cast into sand molds to obtain bars of 45 mm diameter. The alloys were characterized by optical and electron microscopy, and X-ray diffraction. Bulk hardness was measured in the as-cast conditions and after a destabilization heat treatment at 900 °C for 30 min. Abrasive wear resistance tests were undertaken for the different irons according to the ASTM G65 standard in both as-cast and heat-treated conditions under three loads (58, 75 and 93 N). The results show that niobium additions caused a decrease in the carbon content in the alloy and that some carbon is also consumed by forming niobium carbides at the beginning of the solidification process; thus decreasing the eutectic M7C3 carbide volume fraction (CVF) from 30% for the base iron to 24% for the iron with 3.98% Nb. However, the overall carbide content was constant at 30%; bulk hardness changed from 48 to 55 hardness Rockwell C (HRC) and the wear resistance was found to have an interesting behavior. At the lowest load, wear resistance for the base iron was 50% lower than that for the 3.98% Nb iron, which is attributed to the presence of hard NbC. However, at the highest load, the wear behavior was quite similar for all the irons, and it was attributed to a severe carbide cracking phenomenon, particularly in the as-cast alloys. After the destabilization heat treatment, the wear resistance was higher for the 3.98% Nb iron at any load; however, at the highest load, not much difference in wear resistance was observed. Such a behavior is discussed in terms of the carbide volume fraction (CVF), the amount of niobium carbides, the amount of martensite/austenite in matrix and the amount of secondary carbides precipitated during the destabilization heat treatment.
Wear Behavior of Austempered and Quenched and Tempered Gray Cast Irons under Similar Hardness
Authors: Wang Bingxu , Han Xue , Barber Gary C. , Pan Yuming
Published: December 2019

In this research, an austempering heat treatment was applied on gray cast iron using various austempering temperatures ranging from 232 °C to 371 °C and holding times ranging from 1 min to 120 min. The microstructure and hardness were examined using optical microscopy and a Rockwell hardness tester. Rotational ball-on-disk sliding wear tests were carried out to investigate the wear behavior of austempered gray cast iron samples and to compare with conventional quenched and tempered gray cast iron samples under equivalent hardness. For the austempered samples, it was found that acicular ferrite and carbon saturated austenite were formed in the matrix. The ferritic platelets became coarse when increasing the austempering temperature or extending the holding time. Hardness decreased due to a decreasing amount of martensite in the matrix. In wear tests, austempered gray cast iron samples showed slightly higher wear resistance than quenched and tempered samples under similar hardness while using the austempering temperatures of 232 °C, 260 °C, 288 °C, and 316 °C and distinctly better wear resistance while using the austempering temperatures of 343 °C and 371 °C. After analyzing the worn surface, abrasive wear and fatigue wear with the presence of pits, spalls, voids, long cracks, and wear debris were the main mechanisms for austempered gray cast iron with a low austempering temperature. However, only small pits and short cracks were observed on the wear track of austempered gray cast iron with high austempering temperature. Furthermore, the graphite flakes were exposed and ground by the counterpart surface during wear tests. Then, the graphite particles would form a tribo-layer to protect the contact surface.
Light Extraction Analysis of AlGaInP Based Red and GaN Based Blue/Green Flip-Chip Micro-LEDs Using the Monte Carlo Ray Tracing Method
Authors: Lan Shuyu , Wan Hui , Zhao Jie , Zhou Shengjun
Published: December 2019

Micro-scale light emitting diodes (micro-LEDs) commonly employ a thin-film flip-chip (TFFC) structure whose substrate is lifted off by an excimer laser. However, flip-chip (FC) micro-LEDs with a substrate can provide a sharp rise on sidewall emission by increasing the sidewall area. Here, we investigate the influence of substrate thickness, encapsulation, surface texture, microstructures between the substrate and epilayer, as well as the size, cutting shape, and angle of the chip on the light extraction efficiencies (LEEs) of FC micro-LEDs by using the Monte Carlo ray tracing method. We find that the LEE of the blue FC micro-LED chip increases by 46.5% over that of the blue TFFC micro-LED chip. After the encapsulation with the epoxy lens is applied, the LEEs of the blue TFFC micro-LED and blue FC micro-LED increase by 129% and 110.5%, respectively. The underlying mechanisms for the use of surface texture, patterned sapphire substrate, air-void array, and chip shaping technologies to improve the LEEs of FC micro-LEDs are also investigated in detail. We find that the LEEs AlGaInP based red FC micro-LED and GaN based blue/green FC micro-LEDs exhibit a sharp rise when the chip size drops from 30 to 10 µm. The inverted trapezoid FC micro-LED with patterned sapphire substrate (PSS) and encapsulation shows extraordinarily strong top emission and high collimation. We believe that our study offers a promising and practical route for obtaining high efficiency micro-LEDs.
Clostridioides (Clostridium) Difficile in Food-Producing Animals, Horses and Household Pets: A Comprehensive Review
Authors: Kachrimanidou Melina , Tzika Eleni , Filioussis George
Published: December 2019

Clostridioides (Clostridium) difficile is ubiquitous in the environment and is also considered as a bacterium of great importance in diarrhea-associated disease for humans and different animal species. Food animals and household pets are frequently found positive for toxigenic C. difficile without exposing clinical signs of infection. Humans and animals share common C. difficile ribotypes (RTs) suggesting potential zoonotic transmission. However, the role of animals for the development of human infection due to C. difficile remains unclear. One major public health issue is the existence of asymptomatic animals that carry and shed the bacterium to the environment, and infect individuals or populations, directly or through the food chain. C. difficile ribotype 078 is frequently isolated from food animals and household pets as well as from their environment. Nevertheless, direct evidence for the transmission of this particular ribotype from animals to humans has never been established. This review will summarize the current available data on epidemiology, clinical presentations, risk factors and laboratory diagnosis of C. difficile infection in food animals and household pets, outline potential prevention and control strategies, and also describe the current evidence towards a zoonotic potential of C. difficile infection.
Microbial Community Analyses Associated with Nine Varieties of Wine Grape Carposphere Based on High-Throughput Sequencing
Authors: Zhang Shiwei , Chen Xi , Zhong Qiding , Zhuang Xuliang , Bai Zhihui
Published: December 2019

Understanding the composition of microbials on the grape carposphere may provide direct guidance for the wine brewing. In this work, 16S rRNA and ITS (Internal Transcribed Spacer) fungal amplicon sequencing were performed to investigate the differences of epiphytic microbial communities inhabiting different varieties of wine grape berries. The results showed that the dominated phylum of different wine grape carpospheres were Proteobacteria, Actinomycetes, Firmicutes, Gemmatimonadete, and Bacteroidetes. The dominant bacterial genera of different wine grape varieties were Pseudomonas, Arthrobacter, Bacillus, Pantoea, Planomicrobium, Massilia, Curtobacterium, Corynebacterium, Cellulomonas, Sphingomonas, and Microvirga. The fungal communities of the grapes were dominated by Ascomycota for all nine wine varieties. The dominant fungal genera on grape carposphere were Alternaria, Cladosporium, unclassified Capnodiales, Phoma, Rhodotorula, Cryptococcus, Aureobasidium, and Epicoccum. Community structure exerts a significant impact on bacterial Bray-Curtis dissimilarity on six red grapes and also a significant bacterial impact on three white grapes. Community structure exerts a significant impact on fungal Bray-Curtis dissimilarity on six red grapes but weak or no fungal impact on three white grapes. The results revealed that grape variety plays a significant role in shaping bacterial and fungal community, varieties can be distinguished based on the abundance of several key bacterial and fungal taxa.
A Narrative Review on the Potential of Red Beetroot as an Adjuvant Strategy to Counter Fatigue in Children with Cancer
Authors: Swartz Maria C. , Allen Kaitlyn , Deer Rachel R. , Lyons Elizabeth J. , Swartz Michael D. , Clifford Tom
Published: December 2019

Cancer-related fatigue (CRF) is a debilitating adverse effect among children with cancer and a significant barrier to physical activity (PA) participation. PA interventions are effective at reducing fatigue and improving both quality of life (QOL) and functional outcomes in children with cancer. However, 50–70% of children with cancer do not meet PA guidelines. Thus, adjuvant methods are needed to increase PA participation. Given the growing interest in the use of beetroot juice to reduce exercise-induced fatigue, our narrative review evaluated the potential use of beetroot to improve PA participation to counter CRF and improve QOL. Our review of 249 articles showed a lack of published clinical trials of beetroot in children and adults with cancer. Trials of beetroot use had been conducted in a noncancer population (n = 198), and anticancer studies were primarily in the preclinical phase (n = 40). Although results are promising, with beetroot juice shown to counter exercise-induced fatigue in a variety of athletic and patient populations, its use to counter CRF in children with cancer is inconclusive. Pilot and feasibility studies are needed to examine the potential benefits of beetroot to counter CRF, increase PA participation, and improve QOL in children with cancer.
The Effect of Hops (Humulus lupulus L.) Extract Supplementation on Weight Gain, Adiposity and Intestinal Function in Ovariectomized Mice
Authors: Hamm Alison K. , Manter Daniel K. , Kirkwood Jay S. , Wolfe Lisa M. , Cox-York Kimberly , Weir Tiffany L.
Published: December 2019

Estrogen decline during menopause is associated with altered metabolism, weight gain and increased risk of cardiometabolic diseases. The gut microbiota also plays a role in the development of cardiometabolic dysfunction and is also subject to changes associated with age-related hormone changes. Phytoestrogens are plant-based estrogen mimics that have gained popularity as dietary supplements for the treatment or prevention of menopause-related symptoms. These compounds have the potential to both modulate and be metabolized by the gut microbiota. Hops (Humulus lupulus L.) contain potent phytoestrogen precursors, which rely on microbial biotransformation in the gut to estrogenic forms. We supplemented ovariectomized (OVX) or sham-operated (SHAM) C57BL/6 mice, with oral estradiol (E2), a flavonoid-rich extract from hops, or a placebo carrier oil, to observe effects on adiposity, inflammation, and gut bacteria composition. Hops extract (HE) and E2 protected against increased visceral adiposity and liver triglyceride accumulation in OVX animals. Surprisingly, we found no evidence of OVX having a significant impact on the overall gut bacterial community structure. We did find differences in the abundance of Akkermansia muciniphila, which was lower with HE treatment in the SHAM group relative to OVX E2 treatment and to placebo in the SHAM group.
Effect of Low-Protein Diet and Inulin on Microbiota and Clinical Parameters in Patients with Chronic Kidney Disease
Authors: Lai Silvia , Molfino Alessio , Testorio Massimo , Perrotta Adolfo M. , Currado Annachiara , Pintus Giovanni , Pietrucci Daniele , Unida Valeria , La Rocca Davide , Biocca Silvia , Desideri Alessandro
Published: December 2019

Introduction: The gut microbiota has coevolved with humans for a mutually beneficial coexistence and plays an important role in health and disease. A dysbiotic gut microbiome may contribute to progression to chronic kidney disease (CKD) and CKD-related complications such as cardiovascular disease. Microbiota modulation through the administration of prebiotics may represent an important therapeutic target. Aim: We sought to evaluate the effects of a low-protein diet (LPD) (0.6 g/kg/day) with or without the intake of the prebiotic inulin (19 g/day) on microbiota and clinical parameters in CKD patients. Materials and Methods: We performed a longitudinal, prospective, controlled, and interventional study on 16 patients: 9 patients treated with LPD (0.6 g/kg/day) and inulin (19 g/day) and 7 patients (control group) treated only with LPD (0.6 g/kg/day). Clinical evaluations were performed and fecal samples were collected for a subsequent evaluation of the intestinal microbiota in all patients. These tests were carried out before the initiation of LPD, with or without inulin, at baseline (T0) and at 6 months (T2). The microbiota of 16 healthy control (HC) subjects was also analyzed in order to identify potential dysbiosis between patients and healthy subjects. Results: Gut microbiota of CKD patients was different from that of healthy controls. The LPD was able to significantly increase the frequencies of Akkermansiaceae and Bacteroidaceae and decrease the frequencies of Christensenellaceae, Clostridiaceae, Lactobacillaceae, and Pasteurellaceae. Only Bifidobacteriaceae were increased when the LPD was accompanied by oral inulin intake. We showed a significant reduction of serum uric acid (SUA) and C-reactive protein (CRP) in patients treated with LPD and inulin (p = 0.018 and p = 0.003, respectively), an improvement in SF-36 (physical role functioning and general health perceptions; p = 0.03 and p = 0.01, respectively), and a significant increase of serum bicarbonate both in patients treated with LPD (p = 0.026) or with LPD and inulin (p = 0.01). Moreover, in patients treated with LPD and inulin, we observed a significant reduction in circulating tumor necrosis factor alpha (TNF-α) (p = 0.041) and plasma nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX2) (p = 0.027) levels. We did not find a significant difference in the circulating levels of Interleukin (IL)-1β (p = 0.529) and IL-6 (p = 0.828) in the two groups. Conclusions: LPD, associated or not with inulin, modified gut microbiota and modulated inflammatory and metabolic parameters in patients with CKD. Our results suggest that interventions attempting to modulate the gut microbiome may represent novel strategies to improve clinical outcomes in CKD patients and may provide useful therapeutic effects.
Maternal Decisions on Portion Size and Portion Control Strategies for Snacks in Preschool Children
Authors: Reale Sophie , Marr Colette , Cecil Joanne E. , Hetherington Marion M. , Caton Samantha J.
Published: December 2019

Caregivers are responsible for the type and amount of food young children are served. However, it remains unclear what considerations caregivers make when serving snacks to children. The aim of the study was to explore mothers’ decisions and portion control strategies during snack preparation in the home environment. Forty mothers of children aged 24–48 months participated in the study. Mothers prepared five snack foods for themselves and their child whilst verbalizing their actions and thoughts. Mothers were then asked about their portion size decisions in a semi-structured interview. Transcripts were imported into NVivo and analyzed thematically. Three key themes were identified: (1) portion size considerations, (2) portion control methods, and (3) awareness and use of portion size recommendations. Transient, food-related situational influences influenced mothers and disrupted planning and portion control. Food packaging and dishware size were used as visual cues for portion control; however, these vary widely in their size, thus emphasizing mothers’ uncertainty regarding appropriate portion sizes. Mothers called for portion size information to be accessible, child-centered, and simple. These findings reveal multiple considerations when deciding on the correct snack portion sizes for children. These decisions are complex and vary across situations and time, and according to the types of snacks offered.
Association between Dietary Intake and Autistic Traits in Japanese Working Adults: Findings from the Eating Habit and Well-Being Study
Authors: Nakamura Mieko , Nagahata Tomomi , Miura Ayako , Okada Eisaku , Shibata Yosuke , Ojima Toshiyuki
Published: December 2019

“Autistic traits” include a wide range of severity levels. They are often subclinical, and widely distributed in the general population. It is possible that food selectivity due to hyper- or hypo-reactivity to smell or texture, an autistic feature, may result in inadequate nutrient intakes even among non-clinical adults with autistic traits. However, dietary intake of adults with autistic traits has not been elucidated. This study aimed to investigate an association between dietary intake and autistic traits. We cross-sectionally analyzed data of 1440 men and 613 women extracted from the Eating Habit and Well-Being study. Autistic traits were assessed using the Japanese version of the Subthreshold Autism Trait Questionnaire (SATQ), and dietary intake was assessed using a validated food frequency questionnaire. Iron and vitamin B12 intakes were marginally and inversely associated with the SATQ score in men. The SATQ score in women was positively associated with carbohydrate intake, but had an inverse association with protein, mineral, vitamin, and dietary fiber intakes. Low intakes of seaweed, fish and shellfish, and vegetables were observed in participants with severe autistic traits. Associations of autistic traits with food selectivity and low nutrient intakes should be further investigated to promote a new dietary strategy in the general population.
Investigation of Silicone-Containing Semisolid in Situ Film-Forming Systems Using QbD Tools
Authors: Kis Nikolett , Kovács Anita , Budai-Szűcs Mária , Gácsi Attila , Csányi Erzsébet , Csóka Ildikó , Berkó Szilvia
Published: December 2019

The aim of our research work was to develop dermally applicable, semisolid film-forming systems (FFSs) containing silicones, which form a film on the skin in situ, with suitable mechanical properties for skin application. FFSs were developed and investigated by means of the Quality by Design (QbD) methodology. With this QbD approach, the initial risk assessment defines the critical quality attributes (CQAs), the critical material attributes (CMAs) and the critical process parameters (CPPs) to ensure the required quality. Different semisolid systems were formed with or without silicones. During the initial risk assessment, three CQAs, namely skin adhesion, film flexibility and burst strength, were found to be critical attributes, while film appearance, film integrity and the drying time of the semisolid system, were found to be medium attributes. These parameters were investigated. The initial risk assessment also showed that there are three high CMAs: the type of silicones, film-forming excipients, drying excipients, and that there was one medium CMA: viscosity-enhancing excipients. Based on our results, the silicone content had a great effect on the film-forming systems. Different silicones affected the mechanical properties of the films in varying ways, decreased the drying time and showed promising results regarding the drying mechanism.
Using a Material Library to Understand the Impacts of Raw Material Properties on Ribbon Quality in Roll Compaction
Authors: Yu Jiaqi , Xu Bing , Zhang Kunfeng , Shi Chenfeng , Zhang Zhiqiang , Fu Jing , Qiao Yanjiang
Published: December 2019

The purpose of this study is to use a material library to investigate the effect of raw material properties on ribbon tensile strength (TS) and solid fraction (SF) in the roll compaction (RC) process. A total of 81 pharmaceutical materials, including 53 excipients and 28 natural product powders (NPPs), were characterized by 22 material descriptors and were compacted under five different hydraulic pressures. The transversal and longitudinal splitting behaviors of the ribbons were summarized. The TS-porosity and TS-pressure relationships were used to explain the roll compaction behavior of powdered materials. Through defining the target ribbon quality (i.e., 0.6 ≤ SF ≤ 0.8 and TS ≥ 1 MPa), the roll compaction behavior classification system (RCBCS) was built and 81 materials were classified into three categories. A total of 24 excipients and five NPPs were classified as Category I materials, which fulfilled the target ribbon quality and had less occurrence of transversal splitting. Moreover, the multivariate relationships between raw material descriptors, the hydraulic pressure and ribbon quality attributes were obtained by PLS regression. Four density-related material descriptors and the cohesion index were identified as critical material attributes (CMAs). The multi-objective design space summarizing the feasible material properties and operational region for the RC process were visualized. The RCBCS presented in this paper enables a formulator to perform the initial risk assessment of any new materials, and the data modeling method helps to predict the impact of formulation ingredients on strength and porosity of compacts.