bluuebunny/crossref_metadata_2025 huggingface dataset:
The mushroom body development and learning ability of adult honeybees are influenced by cold exposure during their early pupal stage
Authors: Zhu Chenyu , Li Han , Xu Xinjian , Zhou Shujing , Zhou Bingfeng , Li Xiang , Xu Hongzhi , Tian Yuanmingyue , Wang Yanxin , Chu Yu , Zhang Xianlan , Zhu Xiangjie
Published: April 2023

The honeybees are the most important pollinator in the production of crops and fresh produce. Temperature affects the survival of honeybees, and determines the quality of their development, which is of great significance for beekeeping production. Yet, little was known about how does low temperature stress during development stage cause bee death and any sub-lethal effect on subsequent. Early pupal stage is the most sensitive stage to low temperature in pupal stage. In this study, early pupal broods were exposed to 20°C for 12, 16, 24, and 48 h, followed by incubation at 35°C until emergence. We found that 48 h of low temperature duration cause 70% of individual bees to die. Although the mortality at 12 and 16 h seems not very high, the association learning ability of the surviving individuals was greatly affected. The brain slices of honeybees showed that low temperature treatment could cause the brain development of honeybees to almost stop. Gene expression profiles between low temperature treatment groups (T24, T48) and the control revealed that 1,267 and 1,174 genes were differentially expressed respectively. Functional enrichment analysis of differentially expressed genes showed that the differential expression of Map3k9, Dhrs4, and Sod-2 genes on MAPK and peroxisome signaling pathway caused oxidative damage to the honeybee head. On the FoxO signal pathway, InsR and FoxO were upregulated, and JNK, Akt, and Bsk were downregulated; and on the insect hormone synthesis signal pathway, Phm and Spo genes were downregulated. Therefore, we speculate that low temperature stress affects hormone regulation. It was detected that the pathways related to the nervous system were Cholinergic synapse, Dopaminergic synapse, GABAergic synapse, Glutamatergic synapse, Serotonergic synapse, Neurotrophin signaling pathway, and Synaptic vesicle cycle. This implies that the synaptic development of honeybees is quite possibly greatly affected by low temperature stress. Understanding how low temperature stress affects the physiology of bee brain development and how it affects bee behavior provide a theoretical foundation for a deeper comprehension of the temperature adaptation mechanism that underlies the “stenothermic” development of social insects, and help to improve honeybee management strategies to ensure the healthy of colony.
Isolation and characterization of halotolerant plant growth promoting rhizobacteria from mangrove region of Sundarbans, India for enhanced crop productivity
Authors: Pallavi  , Mishra Rohit Kumar , Sahu Pramod Kumar , Mishra Vani , Jamal Hafiza , Varma Ajit , Tripathi Swati
Published: April 2023

Halotolerant plant growth promoting rhizobacteria (PGPR) are beneficial microorganisms utilized to mitigate the biotic and abiotic stresses in plants. The areas of Sundarban mangroves of West Bengal, India have been reported to be rich in halotolerant microflora, yet major area remains unexplored. The present study, therefore, aims to map down the region-specific native microbial community potent of salt tolerance, plant growth promoting (PGP) activity and antagonistic activity against fungal pathogens. Bacterial samples were isolated from the saline soil of the Sundarban mangroves. A total of 156 bacterial samples were isolated and 20 were screened for their salt tolerance potential. These isolates were characterised using morphological, biochemical, and molecular approaches. Based on 16s rRNA sequencing, they were classified into 4 different genera, including Arthrobacter sp. (01 isolate), Pseudomonas plecoglossicida (01 isolate), Kocuria rosea (01 isolate), and Bacillus (17 isolates). The halotolerant isolates which possessed plant growth promoting traits including phosphate, and zinc solubilization, indole acetic acid production, siderophore, and ammonia generation were selected. Further, the effect of two halotolerant isolates GN-5 and JR-12 which showed most prominent PGP activities was evaluated in pea plant under high salinity conditions. The isolates improved survival by promoting germination (36 to 43%) and root-shoot growth and weight of pea plant in comparison to non-inoculated control plants. In a subsequent dual culture confrontation experiment, both these halo-tolerant isolates showed antagonistic activities against the aggressive root rot disease-causing Macrophomina phaseolina (Tassi) Goid NAIMCC-F-02902. The identified isolates could be used as potential bioagents for saline soils, with potential antagonistic effect on root rot disease. However, further studies at the physiological and molecular level would help to delineate a detail mechanistic understanding of broad-spectrum defence against salinity and potential biotic pathogen.
Insight into the phytochemical profile and antimicrobial activities of Amomum subulatum and Amomum xanthioides: an in vitro and in silico study
Authors: Alruhaili Mohammed H. , Almuhayawi Mohammed S. , Gattan Hattan S. , Alharbi Mohanned Talal , Nagshabandi Mohammed K. , Jaouni Soad K. Al , Selim Samy , AbdElgawad Hamada
Published: April 2023

Introduction

Medicinal plants have been considered as potential source of therapeutics or as starting materials in drugs formulation.

Methods

The current study aims to shed light on the therapeutic potential of the Amomum subulatom and Amomum xanthioides Fruits by analyzing the phytochemical composition of their seeds and fruits using gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC) techniques to determine the presence of bioactive components such as flavonoids, phenols, vitamins, steroids, and essential oils.

Results and Discussion

The protein content is usually higher than the total lipids in both species except the fruit of A. subulatum which contain more lipids than proteins. The total protein contents for A. subulatum were 235.03 ± 21.49 and 227.49 ± 25.82 mg/g dry weight while for A. xanthioides were 201.9 ± 37.79 and 294.99 ± 37.93 mg/g dry weight for seeds and fruit, respectively. The Carvacrol levels in A. subulatum is 20 times higher than that in A. xanthioides. Lower levels of α-Thujene, Phyllanderenes, Ascaridole, and Pinocarvone were also observed in both species. According to DPPH (2,2-diphenylpicrylhydrazyl) assay, seed the extract of A. subulatum exhibited the highest antioxidant activity (78.26±9.27 %) followed by the seed extract of A. xanthioides (68.21±2.56 %). Similarly, FRAP (Ferric Reducing Antioxidant Power) assay showed that the highest antioxidant activity was exhibited by the seed extract of the two species; 20.14±1.11 and 21.18±1.04 µmol trolox g−1 DW for A. subulatum and A. xanthioides, respectively. In terms of anti-lipid peroxidation, relatively higher values were obtained for the fruit extract of A. subulatum (6.08±0.35) and the seed extract of A. xanthioides (6.11±0.55). Ethanolic seed extracts of A. subulatum had the highest efficiency against four Gram-negative bacterial species which causes serious human diseases, namely Pseudomonas aeruginosa, Proteus vulgaris, Enterobacter aerogenes, and Salmonella typhimurium. In addition, P. aeruginosa was also inhibited by the fruit extract of both A. subulatum and A. xanthioides. For the seed extract of A. xanthioides, large inhibition zones were formed against P. vulgaris and the fungus Candida albicans. Finally, we have in silico explored the mode of action of these plants by performing detailed molecular modeling studies and showed that the antimicrobial activities of these plants could be attributed to the high binding affinity of their bioactive compounds to bind to the active sites of the sterol 14-alpha demethylase and the transcriptional regulator MvfR.

Conclusion

These findings demonstrate the two species extracts possess high biological activities and therapeutical values, which increases their potential value in a number of therapeutic applications.
Partitioning of respired CO2 in newly sprouted Moso bamboo culms
Authors: Ye Chongyu , Zeng Qiangfa , Hu Keda , Fang Dongming , Hölscher Dirk , Du Huaqiang , Shi Yongjun , Zhou Yufeng , Berninger Frank , Mei Tingting , Zhou Guomo
Published: April 2023

Stem respiration (Rs) plays a vital role in ecosystem carbon cycling. However, the measured efflux on the stem surface (Es) is not always in situ Rs but only part of it. A previously proposed mass balance framework (MBF) attempted to explore the multiple partitioning pathways of Rs, including sap-flow-transported and internal storage of Rs, in addition to Es. This study proposed stem photosynthesis as an additional partitioning pathway to the MBF. Correspondingly, a double-chamber apparatus was designed and applied on newly sprouted Moso bamboo (Phyllostachys edulis) in leafless and leaved stages. Rs of newly sprouted bamboo were twice as high in the leafless stage (7.41 ± 2.66 μmol m−2 s−1) than in the leaved stage (3.47 ± 2.43 μmol m−2 s−1). Es accounted for ~80% of Rs, while sap flow may take away ~2% of Rs in both leafless and leaved stages. Culm photosynthesis accounted for ~9% and 13% of Rs, respectively. Carbon sequestration from culm photosynthesis accounted for approximately 2% of the aboveground bamboo biomass in the leafless stage. High culm photosynthesis but low sap flow during the leafless stage and vice versa during the leaved stage make bamboo an outstanding choice for exploring the MBF.
Effects of inorganic and compost tea fertilizers application on the taxonomic and functional microbial diversity of the purslane rhizosphere
Authors: Carrascosa Angel , Pascual Jose Antonio , López-García Álvaro , Romo-Vaquero María , De Santiago Ana , Ros Margarita , Petropoulos Spyridon A. , Alguacil Maria Del Mar
Published: April 2023

Introduction

Soil fertility is a major determinant of plant-microbial interactions, thus, directly and indirectly affecting crop productivity and ecosystem functions. In this study, we analysed for the first time the effects of fertilizer addition on the cropping of purslane (Portulaca oleracea) with particular attention to the taxonomic and functional characteristics of their associated soil microbiota.

Methods

We tested the effects of different doses of inorganic fertilization differing in the amount of N:P:K namely IT1 (300:100:100); IT2 (300:200:100); IT3 (300:200:200); and IT4 (600:100:100) (ppm N:P:K ratio) and organic fertilization (compost tea) which reached at the end of the assay the dose of 300 ppm N.

Results and discussion

Purslane growth and soil quality parameters and their microbial community structure, abundance of fungal functional groups and prevailing bacterial metabolic functions were monitored. The application of compost tea and inorganic fertilizers significantly increased the purslane shoot biomass, and some soil chemical properties such as pH and soil enzymatic activities related to C, N and P biogeochemical cycles. The bacterial and fungal community compositions were significantly affected by the organic and chemical fertilizers input. The majority of inorganic fertilization treatments decreased the fungal and bacterial diversity as well as some predictive bacterial functional pathways.

Conclusions

These findings suggest that the inorganic fertilization might lead to a change of microbial functioning. However, in order to get stronger evidence that supports the found pattern, longer time-frame experiments that ideally include sampling across different seasons are needed. Thus, further research is still needed to investigate the effects of fertilizations on purslane productivity under commercial field conditions.
Integrating optical imaging techniques for a novel approach to evaluate Siberian wild rye seed maturity
Authors: Jia Zhicheng , Ou Chengming , Sun Shoujiang , Wang Juan , Liu Jingyu , Sun Ming , Ma Wen , Li Manli , Jia Shangang , Mao Peisheng
Published: April 2023

Advances in optical imaging technology using rapid and non-destructive methods have led to improvements in the efficiency of seed quality detection. Accurately timing the harvest is crucial for maximizing the yield of higher-quality Siberian wild rye seeds by minimizing excessive shattering during harvesting. This research applied integrated optical imaging techniques and machine learning algorithms to develop different models for classifying Siberian wild rye seeds based on different maturity stages and grain positions. The multi-source fusion of morphological, multispectral, and autofluorescence data provided more comprehensive information but also increases the performance requirements of the equipment. Therefore, we employed three filtering algorithms, namely minimal joint mutual information maximization (JMIM), information gain, and Gini impurity, and set up two control methods (feature union and no-filtering) to assess the impact of retaining only 20% of the features on the model performance. Both JMIM and information gain revealed autofluorescence and morphological features (CIELab A, CIELab B, hue and saturation), with these two filtering algorithms showing shorter run times. Furthermore, a strong correlation was observed between shoot length and morphological and autofluorescence spectral features. Machine learning models based on linear discriminant analysis (LDA), random forests (RF) and support vector machines (SVM) showed high performance (>0.78 accuracies) in classifying seeds at different maturity stages. Furthermore, it was found that there was considerable variation in the different grain positions at the maturity stage, and the K-means approach was used to improve the model performance by 5.8%-9.24%. In conclusion, our study demonstrated that feature filtering algorithms combined with machine learning algorithms offer high performance and low cost in identifying seed maturity stages and that the application of k-means techniques for inconsistent maturity improves classification accuracy. Therefore, this technique could be employed classification of seed maturity and superior physiological quality for Siberian wild rye seeds.
Auxin inhibits chlorophyll accumulation through ARF7-IAA14-mediated repression of chlorophyll biosynthesis genes in Arabidopsis
Authors: Luo Wei-Gui , Liang Qi-Wen , Su Yi , Huang Chao , Mo Bei-Xin , Yu Yu , Xiao Lang-Tao
Published: April 2023

Auxin is a well-known important phytohormone in plant that plays vital roles in almost every development process throughout plant lifecycle. However, the effect of auxin on the metabolism of chlorophyll, one of the most important pigments involved in the photosynthesis, was intertwined and the underlying mechanism remained to be explored. Here, we found the auxin-defective yuc2 yuc6 double mutant displayed dark-green leaf color with higher chlorophyll content than wildtype, suggesting a negative regulatory role of auxin in chlorophyll biosynthesis. The chloroplast number and structure in mesophyll cells were altered and the photosynthetic efficiency was improved in yuc2 yuc6. In addition, the chlorophyll level was significantly improved during seedling de-etiolation in yuc2 yuc6 mutant, and decreased dramatically under IAA treatment, confirming the inhibitory role of auxin in chlorophyll biosynthesis. The analyses of gene expression in mature leaves and de-etiolation seedlings suggested that auxin suppressed the expression of many chlorophyll biosynthesis genes, especially PROTOCHLOROPHYLLIDE OXIDOREDUCTASE A (PORA) and GENOMES UNCOUPLED 5 (GUN5). Yeast-one-hybrid and luciferase assays demonstrated that the AUXIN RESPONSE FACTOR 2 (ARF2) and ARF7 bind to the promoter of PORA and GUN5 to suppress their expression with the help of INDOLE-3-ACETIC ACID14 (IAA14). Collectively, our research explicitly unraveled the direct inhibitory role of auxin in chlorophyll biosynthesis, and provided new insight into the interplay between auxin signaling and chlorophyll metabolism.
Quantitative EEG for early differential diagnosis of dementia with Lewy bodies
Authors: Iannaccone Sandro , Houdayer Elise , Spina Alfio , Nocera Gianluca , Alemanno Federica
Published: April 2023

Introduction

Differentiating between the two most common forms of dementia, Alzheimer’s dementia and dementia with Lewy bodies (DLB) remains difficult and requires the use of invasive, expensive, and resource-intensive techniques. We aimed to investigate the sensitivity and specificity of electroencephalography quantified using the statistical pattern recognition method (qEEG-SPR) for identifying dementia and DLB.

Methods

Thirty-two outpatients and 16 controls underwent clinical assessment (by two blinded neurologists), EEG recording, and a 6-month follow-up clinical assessment. EEG data were processed using a qEEG-SPR protocol to derive a Dementia Index (positive or negative) and DLB index (positive or negative) for each participant which was compared against the diagnosis given at clinical assessment. Confusion matrices were used to calculate sensitivity, specificity, and predictive values for identifying dementia and DLB specifically.

Results

Clinical assessment identified 30 cases of dementia, 2 of which were diagnosed clinically with possible DLB, 14 with probable DLB and DLB was excluded in 14 patients. qEEG-SPR confirmed the dementia diagnosis in 26 out of the 32 patients and led to 6.3% of false positives (FP) and 9.4% of false negatives (FN). qEEG-SPR was used to provide a DLB diagnosis among patients who received a positive or inconclusive result of Dementia index and led to 13.6% of FP and 13.6% of FN. Confusion matrices indicated a sensitivity of 80%, a specificity of 89%, a positive predictive value of 92%, a negative predictive value of 72%, and an accuracy of 83% to diagnose dementia. The DLB index showed a sensitivity of 60%, a specificity of 90%, a positive predictive value of 75%, a negative predictive value of 81%, and an accuracy of 75%. Neuropsychological scores did not differ significantly between DLB and non- DLB patients. Head trauma or story of stroke were identified as possible causes of FP results for DLB diagnosis.

Conclusion

qEEG-SPR is a sensitive and specific tool for diagnosing dementia and differentiating DLB from other forms of dementia in the initial state. This non-invasive, low-cost, and environmentally friendly method is a promising diagnostic tool for dementia diagnosis which could be implemented in local care settings.
Exposure to nature is associated with decreased functional connectivity within the distress network: A resting state EEG study
Authors: Imperatori Claudio , Massullo Chiara , De Rossi Elena , Carbone Giuseppe Alessio , Theodorou Annalisa , Scopelliti Massimiliano , Romano Luciano , Del Gatto Claudia , Allegrini Giorgia , Carrus Giuseppe , Panno Angelo
Published: April 2023

Introduction

Despite the well-established evidence supporting the restorative potential of nature exposure, the neurophysiological underpinnings of the restorative cognitive/emotional effect of nature are not yet fully understood. The main purpose of the current study was to investigate the association between exposure to nature and electroencephalography (EEG) functional connectivity in the distress network.

Methods

Fifty-three individuals (11 men and 42 women; mean age 21.38 ± 1.54 years) were randomly assigned to two groups: (i) a green group and (ii) a gray group. A slideshow consisting of images depicting natural and urban scenarios were, respectively, presented to the green and the gray group. Before and after the slideshow, 5 min resting state (RS) EEG recordings were performed. The exact low-resolution electromagnetic tomography (eLORETA) software was used to execute all EEG analyses.

Results

Compared to the gray group, the green group showed a significant increase in positive emotions (F1; 50 = 9.50 p = 0.003) and in the subjective experience of being full of energy and alive (F1; 50 = 4.72 p = 0.035). Furthermore, as compared to urban pictures, the exposure to natural images was associated with a decrease of delta functional connectivity in the distress network, specifically between the left insula and left subgenual anterior cingulate cortex (T = −3.70, p = 0.023).

Discussion

Our results would seem to be in accordance with previous neurophysiological studies suggesting that experiencing natural environments is associated with brain functional dynamics linked to emotional restorative processes.
Validation of the Grief Support in Healthcare Scale among frontline nursing professionals working in COVID-19 inpatient wards in Korea
Authors: Ahn Junseok , Bang Young Rong , Cho Eulah , Ahmed Oli , Kim Jeong Hye , Hong Youjin , Chung Seockhoon , Anderson Keith A.
Published: April 2023

Introduction

During the COVID-19 pandemic, healthcare workers (HCWs) have been exposed to higher levels of anxiety and psychological stress than the general population. Nurses who cared for COVID patients could not avoid repeated mourning as they witnessed the deaths of their patients. Therefore, tools are needed to evaluate whether there is adequate support for the grieving process of HCWs in both qualitative and quantitative manners.

Methods

Data from 229 nurses who witnessed the deaths of COVID-19 inpatients were analyzed using an online survey of nurses working in three tertiary hospitals. Factor analysis was conducted to validate the 10-item Korean version of Grief Support in Healthcare Scale (GSHCS). Stress and Anxiety to Viral Epidemics-9 was used to measure stress and anxiety caused by coronavirus, Generalized Anxiety Disorder-7 was used to measure overall anxiety, and Patient Health Questionnaire-9 was used for depression. Convergent validity correlation analysis was also performed with GSHCS.

Results

The two-factor model showed a good fit for the 10-item GSHCS (χ2 = 35.233, df = 34, p = 0.410, CFI = 0.999, TLI = 0.990, RMSEA = 0.013, SRMR = 0.064). Cronbach’s alpha is 0.918 and McDonald’s omega is 0.913, suggesting that the 10-item version of the GSHCS is reliable for determining psychometric properties.

Conclusion

According to this study, the 10-item Korean version of the GSHCS is a reliable and valid measure of psychological support for grief among frontline nursing professionals who have witnessed the deaths of patients they cared for while working in COVID-19 inpatient wards. A two-factor model of the GSHCS has a good model fit and good convergent validity with other rating scales that measure viral anxiety, depression, and general anxiety.
Efficacy of mindfulness- and acceptance-based cognitive-behavioral therapies for bodily distress in adults: a meta-analysis
Authors: Maas genannt Bermpohl Frederic , Hülsmann Lea , Martin Alexandra
Published: April 2023

Objective

Bodily distress, i.e., somatoform disorders and associated functional somatic syndromes, is highly prevalent, often persistent and highly disabling. It has been proposed that “third wave” therapies may be beneficial variants of cognitive behavioral treatments. However, evidence on their efficacy is scarce. This meta-analysis examines the efficacy of “third wave” psychotherapies (mindfulness-based cognitive therapy [MBCT], mindfulness-based stress reduction [MBSR], and acceptance and commitment therapy [ACT]) in adults with bodily distress.

Method

We included randomized controlled trials (RCTs) treating adults with bodily distress using MBCT, MBSR, and ACT compared to inactive and non-specific control groups. A random effects model was used. The primary outcome was somatic symptom severity. Secondary outcomes were degrees of depression and of anxiety, health anxiety, perceived health status, mindfulness, psychological inflexibility, and pain acceptance.

Results

Sixteen RCTs with 1,288 participants were included in the analysis (k = 4 MBCT, k = 7 MBSR, k = 5 ACT; k = 7 fibromyalgia, k = 5 irritable bowel syndrome, k = 1 chronic fatigue syndrome, k = 2 bodily distress, k = 1 medically unexplained symptoms). However, not all studies provided data for each of the relevant outcomes. The analyses revealed that “third wave“ therapies were more effective than control conditions in reducing somatic symptom severity (k = 15, n = 1,100, g = −0.51, 95%CI −0.69; −0.32). Heterogeneity was moderate (I2 = 52.8%, 95%CI 15.1 to 73.8). Effects for secondary outcomes were small to moderate with varying degrees of heterogeneity. We did not find differences between the different therapy approaches (mindfulness- vs. acceptance-based therapies); neither therapy dosis (i.e., total duration of therapy sessions) nor number of sessions were significant moderators of efficacy.

Conclusions

The therapies addressing mindfulness and acceptance proved to be more effective than control conditions. Hence, they are promising treatment approaches for bodily distress. However, there is still need for research on which patient groups may benefit from these psychological approaches.

Systematic review registration

https://osf.io/g7hby, identifier: 10.17605/OSF.IO/4RZGC.
Pharmacological prevention of postictal agitation after electroconvulsive therapy—A systematic review and meta-analysis
Authors: Feenstra Thomas C. , Blake Yvonne , Hoogendoorn Adriaan W. , Koekenbier Krista , Beekman Aartjan T. F. , Rhebergen Didi
Published: April 2023

Background

Postictal agitation (PIA) after electroconvulsive therapy (ECT) is a serious clinical problem estimated to occur in 7–36% of patients and recur in 19–54% of patients. PIA has the potential to cause dangerous situations for the patient and staff members aside from the financial impact. To date, it is unclear which pharmacological interventions should be used in the management of PIA. This study aimed to systematically review the (preventative) pharmacological treatment options for PIA after ECT.

Method

A systematic search was done in PubMed, EMBASE, PsycINFO, and Web of Science from inception until 10 November 2022. We included randomized trials with any pharmacological intervention or comparison and a predefined outcome measure on PIA. Studies that solely included patients with neurodegenerative disorders or stroke were excluded. Data quality was assessed with the RoB2 and GRADE. Meta-analysis was performed if possible. This study was registered on PROSPERO under CRD42021262323.

Results

We screened 2,204 articles and included 14 studies. Dexmedetomidine was investigated in 10 studies. Alfentanil, lignocaine, esmolol, midazolam, propofol, ketamine, haloperidol, and diazepam were each studied in only one study. Meta-analysis revealed an OR of 0.45 (0.32–0.63), a moderate effect size, in favor of dexmedetomidine than placebo to prevent PIA with very low heterogeneity (I2 = 0%). The certainty of the evidence was moderate. The other interventions studied were all found to have low certainty of evidence.

Conclusion

For clinical practice, we believe that our results indicate that dexmedetomidine should be considered for the prevention of PIA in patients that have previously experienced PIA.
“You knew you had to be there, it had to be done”: Experiences of health professionals who faced the COVID-19 pandemic in one public hospital in Spain
Authors: Rodríguez-Madrid María Nieves , Pastor-Moreno Guadalupe , Albert-Lopez Enrique , Pastor-Valero María
Published: April 2023

Introduction

The COVID-19 pandemic highlighted the lack of a government contingency plan for an effective response to an unexpected health crisis. This study uses a phenomenological approach to explore the experience of healthcare professionals during the first three waves of the COVID-19 pandemic in a public health hospital in the Valencia region, Spain. It assesses the impact on their health, coping strategies, institutional support, organizational changes, quality of care, and lessons learned.

Methods

We carried out a qualitative study with semi-structured interviews with doctors and nurses from the Preventive Medicine, Emergency, and Internal Medicine Services and the Intensive Care Unit, using the Colaizzi’s 7-step data analysis method.

Results

During the first wave, lack of information and leadership led to feelings of uncertainty, fear of infection, and transmission to family members. Continuous organizational changes and lack of material and human resources brought limited results. The lack of space to accommodate patients, along with insufficient training in treating critical patients, and the frequent moving around of healthcare workers, reduced the quality of care. Despite the high levels of emotional stress reported, no sick leave was taken; the high levels of commitment and professional vocation helped in adapting to the intense work rhythms. Healthcare professionals in the medical services and support units reported higher levels of stress, and a greater sense of neglect by their institution than their colleagues in managerial roles. Family, social support, and camaraderie at work were effective coping strategies. Health professionals showed a strong collective spirit and sense of solidarity. This helped them cope with the additional stress and workload that accompanied the pandemic.

Conclusion

In the wake of this experience, they highlight the need for a contingency plan adapted to each organizational context. Such a plan should include psychological counseling and continuous training in critical patient care. Above all, it needs to take advantage of the hard-won knowledge born of the COVID-19 pandemic.
Relationship between tinnitus and olfactory dysfunction: audiovisual, olfactory, and medical examinations
Authors: Katayama Naomi , Yoshida Tadao , Nakashima Tsutomu , Ito Yasuki , Teranishi Masaaki , Iwase Takeshi , Sugiura Saiko , Goto Kensuke , Uchida Yasue , Taki Yosuke , Nakada Takafumi , Tada Ai , Suzuki Hirokazu , Nakano Yuta , Shimono Mariko , Saji Naoki , Kogure Anna , Shimizu Emiko , Sone Michihiko , Hamajima Nobuyuki
Published: April 2023

Introduction

Sensory dysfunctions and cognitive impairments are related to each other. Although a relationship between tinnitus and subjective olfactory dysfunction has been reported, there have been no reports investigating the relationship between tinnitus and olfactory test results.

Methods

To investigate the relationship between tinnitus and olfactory test results, we conducted sensory tests, including hearing and visual examinations. The subjects included 510 community-dwelling individuals (295 women and 215 men) who attended a health checkup in Yakumo, Japan. The age of the subjects ranged from 40 to 91 years (mean ± standard deviation, 63.8 ± 9.9 years). The participants completed a self-reported questionnaire on subjective tinnitus, olfactory function, and hearing function, as well as their lifestyle. The health checkup included smell, hearing, vision, and blood examinations.

Results

After adjusting for age and sex, the presence of tinnitus was significantly associated with subjective olfactory dysfunction, poor olfactory test results, hearing deterioration, vertigo, and headache. Additionally, high serum calcium levels and a low albumin/globulin ratio were significantly associated with low physical activity and nutrition. Women scored higher than men in olfactory and hearing examinations, but there was no gender difference in vision examinations.

Conclusion

Subjective smell dysfunction and poor smell test results were significantly associated with tinnitus complaints. Hearing and vision were associated even after adjusting for age and sex. These findings suggest that evaluating the mutual relationships among sensory organs is important when evaluating the influence of sensory dysfunctions on cognitive function.
A framework for the design, implementation, and evaluation of output-based surveillance systems against zoonotic threats
Authors: Rivers Samantha , Kochanowski Maciej , Stolarek Agnieszka , Ziętek-Barszcz Anna , Horigan Verity , Kent Alexander J. , Dewar Rob
Published: April 2023

Output-based standards set a prescribed target to be achieved by a surveillance system, but they leave the selection of surveillance parameters, such as test type and population to be sampled, to the responsible party in the surveillance area. This allows proportionate legislative surveillance specifications to be imposed over a range of unique geographies. This flexibility makes output-based standards useful in the context of zoonotic threat surveillance, particularly where animal pathogens act as risk indicators for human health or where multiple surveillance streams cover human, animal, and food safety sectors. Yet, these systems are also heavily reliant on the appropriate choice of surveillance options to fit the disease context and the constraints of the organization implementing the surveillance system. Here we describe a framework to assist with designing, implementing, and evaluating output-based surveillance systems showing the effectiveness of a diverse range of activities through a case study example. Despite not all activities being relevant to practitioners in every context, this framework aims to provide a useful toolbox to encourage holistic and stakeholder-focused approaches to the establishment and maintenance of productive output-based surveillance systems.
Subjective wellbeing of preschool children
Authors: Silva Bianca Núbia Souza , Martins Bianca Gonzalez , Campos Lucas Arrais , Marôco João , Campos Juliana Alvares Duarte Bonini
Published: April 2023

Objective

The aim of the present study was to evaluate the psychometric properties of the Autoquestionnaire Qualité de Vie Enfant Imagé (AUQEI) in pre-school children and estimate the influence of demographic characteristics on their subjective wellbeing.

Methods

Construct validity was estimated using confirmatory analysis and the chi-square per degrees of freedom ratio (χ2/df), Comparative Fit Index (CFI), Tucker-Lewis Index (TLI), and Root Mean Square Error of Approximation (RMSEA). Reliability was assessed by the ordinal alpha (α) and omega (ω) coefficients and the factorial invariance by the difference in CFI (ΔCFI). Mean scores for each AUQEI item and the general score were calculated.

Results

A total of 443 Preschool children enrolled in public education institutions participated. The original 4-factor AUQEI model showed collinearity between factors and a high correlation between two items. A single factor model was tested, presenting adequate fit to the data (χ2/df = 4.47; CFI = 0.98; TLI = 0.98; RMSEA = 0.08; α = 0.98; ω = 0.93; UniCo > 0.95, EVC > 0.85, and MIREAL < 0.30) and strict model invariance (ΔCFI < 0.01). The AUQEI model proved to be valid in relation to the external variables. Most children (76.7%) had positive subjective wellbeing. Higher scores were observed for items concerning recreation, holidays, and birthdays, and lower scores for those referring to hospitalization, medication, medical consultation, and being away from the family. The relationship between the demographic characteristics of the child or his/her mother and subjective wellbeing was not significant (p > 0.05).

Conclusions

The assessment of subjective wellbeing with the single-factor AUQEI model provided valid, reliable, and invariant. Thus, being a relevant and interesting instrument to assess wellbeing in young children.
Visible, invisible: Black women in higher education
Authors: Showunmi Victoria
Published: April 2023

This paper explores race and gender in the context of higher education, analyzing the experiences of Black women in academia to create a better understanding of what it is to be Black and a woman in contemporary British society. The main themes of this paper are elaborated through the lens of critical Black feminism. The historical origins of inequalities are outlined foregrounding their influence on how Black women are described and regarded. The damaging impact of everyday and sophisticated racism intersecting with sexism is explored and exemplified. Concepts and theories serve to elucidate the discrimination suffered by Black women in academic roles. White women do not offer unequivocal support to their Black colleagues and may even undermine their career progression. When Black women gain leadership roles, the traditional characteristics associated with leaders often conflict with the stereotypical expectations of Black women. A closer examination of higher education reveals the extent of the racial trauma endured by Black women and the resultant decline in their wellbeing.
Clinical efficacy of PD-1 inhibitor combined with radiotherapy in a multi-drug resistant patient with liver metastasis from gastric cancer
Authors: Xu Judi , Liu Hedai , Ni Guoying , Huang Yan , Huang Ying , Liang Hongxiang , Ni Yufeng , Huang Qian , Yang Zhiyong
Published: April 2023

A 54-year-old male was diagnosed with extensive liver metastasis and small nodule metastasis in the lungs from gastric adenocarcinoma [Her-2 (−)]. The patient achieved significant partial response (PR) after chemotherapy combined with anti-angiogenesis therapy but developed progressive disease (PD) after 5 months. Then, the chemotherapeutic and anti-angiogenic drugs were replaced. Meanwhile, the delivery route of some chemotherapeutic drugs was changed, and some chemotherapeutic drugs were given via transcatheter arterial chemoembolization (TACE) to achieve PR, and PD developed after 3 months of remission maintenance. During chemotherapy combined with anti-angiogenesis, the application of programmed cell death-1 (PD-1) inhibitor achieved PR again and maintained for 5 months before disease progression. The progression of the lesions in the left lobe of the liver and the hepatic hilar lymph nodes was significant. Hence, chemotherapy was terminated and gamma stereotactic body radiation therapy (SBRT) was performed on left lobe lesions and hilar lymph nodes. The lesions both inside and outside the radiation field regressed significantly, reaching PR and abscopal effects. The immune-related adverse events (irAEs) occurred, including erythema and black and luster hair. The abscopal effects of lesion reduction in the radiation field and the enhancement of the immune function stimulated by radiation are a highlight of the combination of radiation and immunotherapy. In the end, the patient died of gastrointestinal failure, with overall survival of 18 months.
Secondary infection of Fasciola gigantica in buffaloes shows a similar pattern of serum cytokine secretion as in primary infection
Authors: Meng Zhen , Zhai Lele , Guo Yanfeng , Zheng Mengwei , Li Liang , Wen Chongli , Zhang Weiyu , Di Wenda
Published: April 2023

Background

As a natural host of Fasciola gigantica, buffalo is widely infected by F. gigantica. Its impact on buffalo production has caused great losses to the husbandry sector, and repeat infection is non-negligible. In buffaloes experimentally infected with F. gigantica, primary and secondary infection have yielded the same rate of fluke recovery, indicating a high susceptibility of buffalo to F. gigantica, which contributes to the high infection rate. Determining the immunological mechanism of susceptibility will deepen the understanding of the interaction between F. gigantica and buffalo. Here, we explored the immune response of buffaloes against primary and secondary F. gigantica infection, with a focus on cytokines’ dynamics explored through serum cytokine detection.

Methods

Buffaloes were assigned to three groups: group A (noninfected, n = 4), group B (primary infection, n = 3), and group C (secondary infection, n = 3). Group B was infected via oral gavage with 250 viable F. gigantica metacercariae, and group C was infected twice with 250 metacercariae at an interval of 4 weeks. The second infection of group C was performed simultaneously with that of group B. Whole blood samples were collected pre-infection (0 weeks) and at 1–6, 10, and 12  weeks after that. The serum levels of seven cytokines (IFN-γ, IL-4, IL-5, IL-10, IL-13, TGF-β, and IL-17) were simultaneously determined using ELISA and further analyzed.

Results

In the present study, no significant changes in Th1-type cytokines production were detected in early infection, both in primary and secondary infections, while the Th2-type response was strongly induced. A comparison of primary and secondary infection showed no significant difference in the cytokine secretion, which may indicate that the re-infection at 4 weeks after primary infection could not induce a robust adaptive immune response. The full extent of interaction between buffalo and F. gigantica in re-infection requires further study.
Molecular detection and characterization of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi infections in dromedary camels (Camelus dromedaries) in Egypt
Authors: Elmahallawy Ehab Kotb , Köster Pamela C. , Dashti Alejandro , Alghamdi Samia Qasem , Saleh Amira , Gareh Ahmed , Alrashdi Barakat M. , Hernández-Castro Carolina , Bailo Begoña , Lokman Maha S. , Hassanen Eman A. A. , González-Barrio David , Carmena David
Published: April 2023

Introduction

Few studies have investigated the occurrence of microeukaryotic gut parasites in dromedary camels in Egypt, and the majority of these investigations are based on microscopic analysis of fecal material.

Methods

Herein, we assessed the occurrence, molecular diversity, and zoonotic potential of protozoan (Cryptosporidium spp. and Giardia duodenalis) and microsporidian (Enterocytozoon bieneusi) pathogens in individual fecal samples (n = 102) of dromedary camels with (n = 26) and without (n = 76) diarrhea from Aswan Governorate, Upper Egypt. Other factors possibly associated with an increased risk of infection (geographical origin, sex, age, and physical condition) were also analyzed. The SSU rRNA or ITS genes were targeted by molecular (PCR and Sanger sequencing) techniques for pathogen detection and species identification.

Results and discussion

The most abundant species detected was G. duodenalis (3.9%, 4/102; 95% CI: 1.1–9.7), followed by Cryptosporidium spp. (2.9%, 3/102; 95% CI: 0.6–8.4). All samples tested negative for the presence of E. bieneusi. Sequence analysis data confirmed the presence of zoonotic C. parvum (66.7%, 2/3) and cattle-adapted C. bovis (33.3%, 1/3). These Cryptosporidium isolates, as well as the four Giardia-positive isolates, were unable to be amplified at adequate genotyping markers (Cryptosporidium: gp60; Giardia: gdh, bg, and tpi). Camels younger than 2 years old were significantly more likely to harbor Cryptosporidium infections. This connection was not statistically significant, although two of the three cryptosporidiosis cases were detected in camels with diarrhea. The spread of G. duodenalis infections was unaffected by any risk variables studied. This is the first report of C. parvum and C. bovis in Egyptian camels. The finding of zoonotic C. parvum has public health implications since camels may function as sources of oocyst pollution in the environment and potentially infect livestock and humans. Although preliminary, this study provides useful baseline data on the epidemiology of diarrhea-causing microeukaryotic parasites in Egypt. Further research is required to confirm and expand our findings in other animal populations and geographical regions of the country.
The effects of obesity and insulin dysregulation on mare reproduction, pregnancy, and foal health: a review
Authors: Hallman Isa , Karikoski Ninja , Kareskoski Maria
Published: April 2023

Obesity is a growing welfare concern in modern equine populations and predisposes horses to disturbances in energy metabolism such as insulin dysregulation. However, equine metabolic syndrome has only been recognized in recent decades. Functioning energy metabolism is pivotal to normal body homeostasis and affects essentially all organ systems, including reproduction. Previous literature suggests that obesity has an effect not only on the reproductive processes in mares but also on offspring health, predisposing the offspring to later-onset orthopedic and metabolic problems. This review focuses on the effects of obesity, insulin dysregulation and hyperinsulinemia on the reproductive functions of mares and the implications on foal health before and after birth. The points of interest are the cyclicity and ovarian function, uterine environment, gestation, the postpartum period, and the newborn foal. The aim is to review the current state of knowledge, and identify outstanding questions that could stimulate future research. This topic is important not only from the equine industry and production perspective but is also relevant for the welfare of future populations and individuals.
A Review of Rehabilitative and Assistive Technologies for Upper-Body Exoskeletal Devices
Authors: Hays Emilly , Slayton Jack , Tejeda-Godinez Gary , Carney Emily , Cruz Kobe , Exley Trevor , Jafari Amir
Published: April 2023

This journal review article focuses on the use of assistive and rehabilitative exoskeletons as a new opportunity for individuals with diminished mobility. The article aims to identify gaps and inconsistencies in state-of-the-art assistive and rehabilitative devices, with the overall goal of promoting innovation and improvement in this field. The literature review explores the mechanisms, actuators, and sensing procedures employed in each application, specifically focusing on passive shoulder supports and active soft robotic actuator gloves. Passive shoulder supports are an excellent option for bearing heavy loads, as they enable the load to be evenly distributed across the shoulder joint. This, in turn, reduces stress and strain around the surrounding muscles. On the other hand, the active soft robotic actuator glove is well suited for providing support and assistance by mimicking the characteristics of human muscle. This review reveals that these devices improve the overall standard of living for those who experience various impairments but also encounter limitations requiring redress. Overall, this article serves as a valuable resource for individuals working in the field of assistive and rehabilitative exoskeletons, providing insight into the state of the art and potential areas for improvement.
Gender-Based Violence in Girls’ Sports
Authors: Willson Erin , Kerr Gretchen
Published: April 2023

Millions of girls and young women participate in organized sports annually as a vehicle for developing a strong sense of self, social bonds, a positive body image and a sense of agency. Although the benefits of sport engagement are experienced by many girls, the overwhelming evidence of experiences of gender-based violence in sport cannot be ignored (e.g., USA Gymnastics, Hockey Canada). This paper will address gender-based violence experienced by adolescents in sport with a focus on psychological violence. The literature is replete with evidence that girls experience higher rates of gender-based psychological violence in sport than boys, and as a result, incur developmental costs. Psychological violence is experienced by girls in sport in the form of demeaning comments, body shaming, inequitable media coverage and funding and the ongoing policing of women’s bodies in sport through sexualized sport attire and physiological testing. The causes and effects of psychological violence will be addressed along with recommendations to prevent and address gender-based violence in sport.