bluuebunny/crossref_metadata_2025 huggingface dataset:
Thymic T-Cell Production Is Associated With Changes in the Gut Microbiota in Young Chicks
Authors: Cheng Jiaheng , Yuan Yushan , Zhao Fang , Chen Jianwei , Chen Peng , Li Ying , Yan Xia , Luo Chenglong , Shu Dingming , Qu Hao , Ji Jian
Published: September 2021

Increasing studies show that gut microbiota play a central role in immunity, although the impact of the microbiota on mediation of thymic T cells throughout life is not well understood. Chickens have been shown to be a valuable model for studying basic immunology. Here, we show that changes in the gut microbiota are associated with the development of thymic T cells in young chickens. Our results showed that T-cell numbers in newborn chicks sharply increased from day 0 and peaked at day 49. Interestingly, the α-diversity score pattern of change in gut microbiota also increased after day 0 and continued to increase until day 49. We found that early antibiotic treatment resulted in a dramatic reduction in gut alpha diversity: principal component analysis (PCA) showed that antibiotic treatment resulted in a different cluster from the controls on days 9 and 49. In the antibiotic-treated chickens, we identified eight significantly different (p < 0.05) microbes at the phylum level and 14 significantly different (p < 0.05) microbes at the genus level, compared with the controls. Importantly, we found that antibiotic treatment led to a decreased percentage and number of T cells in the thymus when measured at days 9 and 49, as evaluated by flow cytometry. Collectively, our data suggest that intestinal microbiota may be involved in the regulation of T cells in birds, presenting the possibility that interventions that actively modify the gut microbiota in early life may accelerate the maturation of humoral immunity, with resulting anti-inflammatory effects against different pathogens.
Mast Cell Modulation of B Cell Responses: An Under-Appreciated Partnership in Host Defence
Authors: Palma Alejandro M. , Hanes Mark R. , Marshall Jean S.
Published: September 2021

Mast cells are well known to be activatedviacross-linking of immunoglobulins bound to surface receptors. They are also recognized as key initiators and regulators of both innate and adaptive immune responses against pathogens, especially in the skin and mucosal surfaces. Substantial attention has been given to the role of mast cells in regulating T cell function either directly or indirectly through actions on dendritic cells. In contrast, the ability of mast cells to modify B cell responses has been less explored. Several lines of evidence suggest that mast cells can greatly modify B cell generation and activities. Mast cells co-localise with B cells in many tissue settings and produce substantial amounts of cytokines, such as IL-6, with profound impacts on B cell development, class-switch recombination events, and subsequent antibody production. Mast cells have also been suggested to modulate the development and functions of regulatory B cells. In this review, we discuss the critical impacts of mast cells on B cells using information from both clinical and laboratory studies and consider the implications of these findings on the host response to infections.
Nasal Immunization With Small Molecule Mast Cell Activators Enhance Immunity to Co-Administered Subunit Immunogens
Authors: Johnson-Weaver Brandi T. , Choi Hae Woong , Yang Hang , Granek Josh A. , Chan Cliburn , Abraham Soman N. , Staats Herman F.
Published: September 2021

Mast cell activators are a novel class of mucosal vaccine adjuvants. The polymeric compound, Compound 48/80 (C48/80), and cationic peptide, Mastoparan 7 (M7) are mast cell activators that provide adjuvant activity when administered by the nasal route. However, small molecule mast cell activators may be a more cost-efficient adjuvant alternative that is easily synthesized with high purity compared to M7 or C48/80. To identify novel mast cell activating compounds that could be evaluated for mucosal vaccine adjuvant activity, we employed high-throughput screening to assess over 55,000 small molecules for mast cell degranulation activity. Fifteen mast cell activating compounds were down-selected to five compounds based onin vitroimmune activation activities including cytokine production and cellular cytotoxicity, synthesis feasibility, and selection for functional diversity. These small molecule mast cell activators were evaluated forin vivoadjuvant activity and induction of protective immunity against West Nile Virus infection in BALB/c mice when combined with West Nile Virus envelope domain III (EDIII) protein in a nasal vaccine. We found that three of the five mast cell activators, ST101036, ST048871, and R529877, evoked high levels of EDIII-specific antibody and conferred comparable levels of protection against WNV challenge. The level of protection provided by these small molecule mast cell activators was comparable to the protection evoked by M7 (67%) but markedly higher than the levels seen with mice immunized with EDIII alone (no adjuvant 33%). Thus, novel small molecule mast cell activators identified by high throughput screening are as efficacious as previously described mast cell activators when used as nasal vaccine adjuvants and represent next-generation mast cell activators for evaluation in mucosal vaccine studies.
Screening and Quantification of Micro(Nano)Plastics and Plastic Additives in the Seawater of Mar Menor Lagoon
Authors: Vega-Herrera Albert , Llorca Marta , Savva Katerina , León Víctor M. , Abad Esteban , Farré Marinella
Published: September 2021

In this work a suspect-screening approach was employed to assess the polymers and plastic additives of micro(nano)plastics (NPL/MPLs) of size ranges from the nm range to 20 μm present in seawater from the top 5 cm of the Mar Menor lagoon during two sampling campaigns (summer and winter), as well of other potentially adsorbed compounds onto the plastic particles surfaces and suspended material. The identification of NPL/MPLs has been based on characteristic Kendrick Mass Defect analysis for each polymer type in mass spectra. The applied methodology allowed to identify NPLs/MPLs of polystyrene (PS), polyethylene (PE), polyisoprene (PI), polybutadiene (PBD), polypropylene (PP), polyamides (PA), polyvinylchloride (PVC), n-isopropylacrylamide (PNIPAm), and polydimethylsiloxanes. In addition, PS, PE, PI, PBD, PP, PA, and PVC were confirmed with standards, and the equivalent concentrations were quantified. The results of this study showed that most frequently found compounds were PP, PE, PA and PNIPAm, while the compound found at higher concentrations was by far PP reaching the 9,303 ± 366 ng/mL in one of the samples. A total number of 135 chemical compounds were tentatively identified, 74 of them plastic additives and compounds used in the polymers manufacture or coming from the polymer’s decomposition. In relation to plastic additives, the more frequently tentatively identified compounds were plasticizers such as phthalates group; stabilizers such as antioxidants (e.g., distearyl 3,3′-thiodipropionate, 2,5-di-tert-butylhydroquinone), and UV filters as benzotriazoles. Several flame retardants of the group of phosphates were as well detected. The other compounds tentatively identified in the samples were pharmaceuticals, pesticides, food additives, flavors and natural products that were attached onto the plastic particles and particulate matter from surrounding waters. In regards to the seasonal variation, during the summer a major number of compounds were tentatively detected, while de concentrations of polymers were slightly higher in winter. The spatial distribution showed higher contamination in the southern part of the coastal lagoon.
Summer Phytoplankton Blooms Induced by Upwelling in the Western South China Sea
Authors: Chen Ying , Shi Haiyi , Zhao Hui
Published: September 2021

During summer, phytoplankton blooms occur frequently off the southeast Vietnam coast in the western South China Sea (WSCS). Using multi-source remote sensing data, such as sea surface temperature (SST), sea surface wind (SSW), aerosol optical thickness (AOT), sea level anomalies (SLA), and chlorophyll-a (Chl-a) data from 1998 to 2020 and in situ observations of analyzed data, we report the patterns of spatial variation of summer phytoplankton blooms in this region. The partial correlation and multiple stepwise linear regression analyses reveal that Ekman transport (ET) and Ekman pumping velocity (EPV) exert a greater impact on the summer phytoplankton blooms than the other environmental factors, suggesting that the phytoplankton blooms in the region may be mainly triggered by the enhanced nutrients by wind-induced upwelling and vertical mixing. AOT only weakly correlates with Chl-a concentration in the region, probably these prominent abundant nutrients in the region come from upwelling and convective-overturn. A northeastward jet causes the distribution of high Chl-a in the WSCS to be plume-shaped. A new finding in this study is that the northward current in this area may cause the northward deviation of phytoplankton blooms from the areas of upwelling.
Raman Spectra of Bulk and Few-Layer GeSe From First-Principles Calculations
Authors: Zhao Yi-Feng , Guan Zhao , Zhong Ni , Yue Fang-Yu , Xiang Ping-Hua , Duan Chun-Gang
Published: September 2021

Raman spectra play a significant role in the study of polar materials. Herein, we report the influence of strain and interlayer shift on vibration responses in bulk and few-layer ferrovalley material GeSe in different polarization states (ferroelectric/FE and antiferroelectric/AFE) based on density functional theory and density functional perturbation theory calculations. We find Ag1 mode shifts by about 10 cm−1 from monolayer to bilayer and trilayer due to the interlayer coupling. The Ag3 mode on behalf of FE mode is observed that is consistent with the experiments in bulk and few-layer GeSe. Meanwhile, in our calculations, with the transition between AFE and FE state in the bilayer and trilayer, the Raman frequency of Ag2 and Ag3 mode decrease obviously whereas that of Ag1 mode increases. Interestingly, the Raman peaks shifted a lot due to the strain effect. We expect these variations in the Raman spectroscopy can be employed to identify the status of GeSe films, e.g., the AFE or FE state, and the number of layers in experiments.
Successful Endoscopic Resection of Primary Rectal Mucosa-Associated Lymphoid Tissue Lymphoma by Endoscopic Submucosal Dissection: A Case Report
Authors: Han Jian , Zhu Zhe , Zhang Chao , Xie Hua-ping
Published: September 2021

Mucosa-associated lymphoid tissue (MALT) lymphoma arises in extra-nodal sites from the malignant transformation of B lymphocytes that are mainly triggered by infection or autoimmune process. MALT lymphoma is frequently detected in the gastrointestinal tract. As the causal relationship between Helicobacter pylori (H. pylori) infection and gastric MALT lymphoma, it was well-established that early-stage gastric MALT lymphoma could be cured by H. pylori eradication, and about 50–95% of cases achieved complete response with anti-H. pylori treatment. Compared to the stomach which is the most involved site due to the high prevalence of H. pylori infection, the colorectum is rarely affected. Primary rectal MALT lymphoma is a rare malignancy, and there are no specific therapeutic strategies so far. Here we report a case of rectal MALT lymphoma successfully resected by endoscopic submucosal dissection (ESD). ESD serves as a novel strategy to cure small localized rectal MALT lymphomas to avoid unnecessary surgery or chemo-radiotherapy.
Podocyte Foot Process Effacement Precedes Albuminuria and Glomerular Hypertrophy in CD2-Associated Protein Deficient Mice
Authors: Basgen John M. , Wong Jenny S. , Ray Justina , Nicholas Susanne B. , Campbell Kirk N.
Published: September 2021

Background: Podocyte foot process effacement is a key histologic finding in proteinuric kidney disease. We previously showed that 3-week old CD2AP-deficient mice have significant proteinuria, glomerular hypertrophy and mesangial expansion. The goal of this study is to use morphometry to establish the temporal sequence of podocyte foot process effacement, glomerular volume expansion and albuminuria in Cd2ap−/− mice by measuring these parameters at the 2-week time point.

Methods: Wild-type mice age 14 ± 1 days with the Cd2ap gene (WT, N = 5) and mice deficient for Cd2ap (Cd2ap KO, N = 5) were generated. Kidneys were harvested and fixed in 2.5% glutaraldehyde and processed for examination by light and electron microscopy. An average of 415.2 (range 268–716) grid points were counted for all the glomeruli, and quantification of glomerular volume from each kidney. Urine was collected the day prior to sacrifice for urine albumin-to-creatinine ratio (ACR) measurements.

Results: There was no difference in albuminuria [median (range) mg/g] between WT [212.2 (177.6–388.4) mg/g] vs. Cd2ap KO mice [203.3 (164.7–910.2) mg/g], P = 0.89; or glomerular volume 68,307[10,931] vs. 66,844[13,022] μm3, p = 0.92. The volume densities of glomerular components of the podocyte, capillary lumen and mesangium were not different for the two groups, P = 0.14, 0.14 and 0.17 respectively. However, foot process width was increased in Cd2ap KO 1128[286] vs. WT [374 ± 42] nm, P = 0.02.

Conclusion: Here we show that while 2-week old WT and Cd2ap KO mice have similar levels of albuminuria, glomerular and mesangial volume, Cd2ap KO mice have more extensive podocyte foot process effacement. The data suggests that podocyte injury is the initiating event leading to mesangial expansion and albuminuria in this model.
Quinolone Signals Related to Pseudomonas Quinolone Signal-Quorum Sensing Inhibits the Predatory Activity of Bdellovibrio bacteriovorus
Authors: Hoshiko Yuki , Nishiyama Yoshito , Moriya Tae , Kadokami Kiwao , López-Jácome Luis Esaú , Hirano Ryutaro , García-Contreras Rodolfo , Maeda Toshinari
Published: September 2021

Bdellovibrio bacteriovorus is one of the predatory bacteria; therefore, it can act as a novel “living antibiotic,” unlike the current antibiotics. Here the predation of Escherichia coli by B. bacteriovorus was inhibited in the presence of Pseudomonas aeruginosa. This study investigated whether P. aeruginosa-induced predation inhibition is associated with bacterial quorum sensing (QS). Each las, rhl, or pqs QS mutant in P. aeruginosa was used to check the predatory activity of E. coli cells using B. bacteriovorus. As a result, the predatory activity of B. bacteriovorus increased in a mutant pqs QS system, whereas wild-type PA14 inhibited the predatory activity. Moreover, the addition of 4-hydroxy-2-heptylquinoline (HHQ) or the analog triggered the low predatory activity of B. bacteriovorus and killed B. bacteriovorus cells. Therefore, a defensive action of P. aeruginosa against B. bacteriovorus is activated by the pqs QS system, which produces some quinolone compounds such as HHQ.
Ketamine: Neuroprotective or Neurotoxic?
Authors: Choudhury Divya , Autry Anita E. , Tolias Kimberley F. , Krishnan Vaishnav
Published: September 2021

Ketamine, a non-competitive N-methyl-D-aspartate receptor (NMDAR) antagonist, has been employed clinically as an intravenous anesthetic since the 1970s. More recently, ketamine has received attention for its rapid antidepressant effects and is actively being explored as a treatment for a wide range of neuropsychiatric syndromes. In model systems, ketamine appears to display a combination of neurotoxic and neuroprotective properties that are context dependent. At anesthetic doses applied during neurodevelopmental windows, ketamine contributes to inflammation, autophagy, apoptosis, and enhances levels of reactive oxygen species. At the same time, subanesthetic dose ketamine is a powerful activator of multiple parallel neurotrophic signaling cascades with neuroprotective actions that are not always NMDAR-dependent. Here, we summarize results from an array of preclinical studies that highlight a complex landscape of intracellular signaling pathways modulated by ketamine and juxtapose the somewhat contrasting neuroprotective and neurotoxic features of this drug.
In silico Identification of Key Factors Driving the Response of Muscle Sensory Neurons to Noxious Stimuli
Authors: Nagaraja Sridevi , Queme Luis F. , Hofmann Megan C. , Tewari Shivendra G. , Jankowski Michael P. , Reifman Jaques
Published: September 2021

Nociceptive nerve endings embedded in muscle tissue transduce peripheral noxious stimuli into an electrical signal [i.e., an action potential (AP)] to initiate pain sensations. A major contributor to nociception from the muscles is mechanosensation. However, due to the heterogeneity in the expression of proteins, such as ion channels, pumps, and exchangers, on muscle nociceptors, we currently do not know the relative contributions of different proteins and signaling molecules to the neuronal response due to mechanical stimuli. In this study, we employed an integrated approach combining a customized experimental study in mice with a computational model to identify key proteins that regulate mechanical nociception in muscles. First, using newly collected data from somatosensory recordings in mouse hindpaw muscles, we developed and then validated a computational model of a mechanosensitive mouse muscle nociceptor. Next, by performing global sensitivity analyses that simulated thousands of nociceptors, we identified three ion channels (among the 17 modeled transmembrane proteins and four endoplasmic reticulum proteins) as potential regulators of the nociceptor response to mechanical forces in both the innocuous and noxious range. Moreover, we found that simulating single knockouts of any of the three ion channels, delayed rectifier voltage-gated K+ channel (Kv1.1) or mechanosensitive channels Piezo2 or TRPA1, considerably altered the excitability of the nociceptor (i.e., each knockout increased or decreased the number of triggered APs compared to when all channels were present). These results suggest that altering expression of the gene encoding Kv1.1, Piezo2, or TRPA1 might regulate the response of mechanosensitive muscle nociceptors.
Identification of Epileptic EEG Signals Through TSK Transfer Learning Fuzzy System
Authors: Zheng Zhaoliang , Dong Xuan , Yao Jian , Zhou Leyuan , Ding Yang , Chen Aiguo
Published: September 2021

We propose a new model to identify epilepsy EEG signals. Some existing intelligent recognition technologies require that the training set and test set have the same distribution when recognizing EEG signals, some only consider reducing the marginal distribution distance of the data while ignoring the intra-class information of data, and some lack of interpretability. To address these deficiencies, we construct a TSK transfer learning fuzzy system (TSK-TL) based on the easy-to-interpret TSK fuzzy system the transfer learning method. The proposed model is interpretable. By using the information contained in the source domain and target domains more effectively, the requirements for data distribution are further relaxed. It realizes the identification of epilepsy EEG signals in data drift scene. The experimental results show that compared with the existing algorithms, TSK-TL has better performance in EEG recognition of epilepsy.
Immunomodulatory Effect of Irreversible Electroporation Alone and Its Cooperating With Immunotherapy in Pancreatic Cancer
Authors: Tian Guo , Guan Jiajia , Chu Yanhua , Zhao Qiyu , Jiang Tian’an
Published: September 2021

Emerging studies have showed irreversible electroporation (IRE) focused on pancreatic cancer (PC). However, the effects of IRE treatment on the immune response of PC remain unknown. Moreover, there are few studies on the therapeutic effect of IRE combining with immunotherapy on PC. Thus, we review recent advances in our understanding of IRE alone and its working with immunotherapy towards the immune response of PC, discussing potential opportunities for exploring future treatment strategies.
Consecutive Serial Non-Contrast CT Scan-Based Deep Learning Model Facilitates the Prediction of Tumor Invasiveness of Ground-Glass Nodules
Authors: Xu Yao , Li Yu , Yin Hongkun , Tang Wen , Fan Guohua
Published: September 2021

Introduction

Tumors are continuously evolving biological systems which can be monitored by medical imaging. Previous studies only focus on single timepoint images, whether the performance could be further improved by using serial noncontrast CT imaging obtained during nodule follow-up management remains unclear. In this study, we evaluated DL model for predicting tumor invasiveness of GGNs through analyzing time series CT images

Methods

A total of 168 pathologically confirmed GGN cases (48 noninvasive lesions and 120 invasive lesions) were retrospectively collected and randomly assigned to the development dataset (n = 123) and independent testing dataset (n = 45). All patients underwent consecutive noncontrast CT examinations, and the baseline CT and 3-month follow-up CT images were collected. The gross region of interest (ROI) patches containing only tumor region and the full ROI patches including both tumor and peritumor regions were cropped from CT images. A baseline model was built on the image features and demographic features. Four DL models were proposed: two single-DL model using gross ROI (model 1) or full ROI patches (model 3) from baseline CT images, and two serial-DL models using gross ROI (model 2) or full ROI patches (model 4) from consecutive CT images (baseline scan and 3-month follow-up scan). In addition, a combined model integrating serial full ROI patches and clinical information was also constructed. The performance of these predictive models was assessed with respect to discrimination and clinical usefulness.

Results

The area under the curve (AUC) of the baseline model, models 1, 2, 3, and 4 were 0.562 [(95% confidence interval (C)], 0.406~0.710), 0.693 (95% CI, 0.538–0.822), 0.787 (95% CI, 0.639–0.895), 0.727 (95% CI, 0.573–0.849), and 0.811 (95% CI, 0.667–0.912) in the independent testing dataset, respectively. The results indicated that the peritumor region had potential to contribute to tumor invasiveness prediction, and the model performance was further improved by integrating imaging scans at multiple timepoints. Furthermore, the combined model showed best discrimination ability, with AUC, sensitivity, specificity, and accuracy achieving 0.831 (95% CI, 0.690–0.926), 86.7%, 73.3%, and 82.2%, respectively.

Conclusion

The DL model integrating full ROIs from serial CT images shows improved predictive performance in differentiating noninvasive from invasive GGNs than the model using only baseline CT images, which could benefit the clinical management of GGNs.
Treosulfan-Based Conditioning Regimen Prior to Allogeneic Stem Cell Transplantation: Long-Term Results From a Phase 2 Clinical Trial
Authors: Lazzari Lorenzo , Ruggeri Annalisa , Lupo Stanghellini Maria Teresa , Mastaglio Sara , Messina Carlo , Giglio Fabio , Lorusso Alessandro , Perini Tommaso , Piemontese Simona , Marcatti Magda , Lorentino Francesca , Xue Elisabetta , Clerici Daniela , Corti Consuelo , Bernardi Massimo , Assanelli Andrea , Greco Raffaella , Ciceri Fabio , Peccatori Jacopo
Published: September 2021

Introduction

Reducing toxicities while preserving efficacy in allogeneic stem cell transplant (allo-HCT) remains a particularly challenging problem. Different strategies to enhance the antitumor activity without increasing early and late adverse toxicities of the conditioning regimens have been investigated.

Methods

The aim of “AlloTreo” prospective phase 2 clinical trial was to evaluate the efficacy and safety of a conditioning regimen based on Treosulfan (42 g/m2) and fludarabine (https://clinicaltrials.gov/ct2/show/NCT00598624). We enrolled 108 patients with hematological diseases who received a first allo-HCT between June 2005 and January 2011, inside the frame of this trial at our center. Median age at allo-HCT was 49 (21–69) years. Disease Risk Index was low in 14 (13%) patients, intermediate in 73 (67.7%), high in 17 (15.7%), and very high in 4 (3.7%). Donors were human leukocyte antigen (HLA)-matched related in 50 cases, 10/10-matched unrelated in 36, and 9/10-mismatched unrelated in 22. Graft-versus-host disease (GvHD) prophylaxis consisted of cyclosporine-A and methotrexate. Anti-T-lymphocyte globulin (ATLG) was administered in patients receiving unrelated allo-HCT. Stem cell source was mainly peripheral blood stem cells (95%).

Results

Conditioning regimen was well tolerated. Full donor chimerism was documented for most patients (88%) at day +30. At 12 years, overall survival (OS) was 41.7% (32.2%–50.9%), progression-free survival (PFS) was 31.7% (23%–40.7%), GvHD-free/relapse-free survival was 20.9% (13.7%–29.1%), cumulative incidence (CI) of relapse was 44.5% (34.9%–53.6%), and transplant-related mortality (TRM) was 22.5% (15.1%–30.9%). CI of acute GvHD grades II–IV was 27.8% (19.7%–36.5%) at 100 days; 12-year CI of chronic GvHD was 40.7% (31.3%–49.9%). Relevant long-term adverse effects were 10 secondary malignancy, 3 fatal cardiovascular events, and 1 late-onset transplant-associated thrombotic microangiopathy. Ten successful pregnancies were reported after allo-HCT. In multivariate analysis, older age (≥60 years) at transplant [hazard ratio (HR), 2.157; p = 0.004] and a high/very high disease risk index (HR, 1.913; p = 0.026) were significantly associated with a lower OS.

Conclusions

Overall, our data confirmed the myeloablative potential and safe toxicity profile of full dose Treo (42 g/m2) especially for the younger population.
Effects of High-Fat and High-Fat/High-Sucrose Diet-Induced Obesity on PVAT Modulation of Vascular Function in Male and Female Mice
Authors: Victorio Jamaira A. , Guizoni Daniele M. , Freitas Israelle N. , Araujo Thiago R. , Davel Ana P.
Published: September 2021

Increased adiposity in perivascular adipose tissue (PVAT) has been related to vascular dysfunction. High-fat (HF) diet-induced obesity models are often used to analyze the translational impact of obesity, but differences in sex and Western diet type complicate comparisons between studies. The role of PVAT was investigated in small mesenteric arteries (SMAs) of male and female mice fed a HF or a HF plus high-sucrose (HF + HS) diet for 3 or 5 months and compared them to age/sex-matched mice fed a chow diet. Vascular responses of SMAs without (PVAT-) or with PVAT (PVAT+) were evaluated. HF and HF + HS diets increased body weight, adiposity, and fasting glucose and insulin levels without affecting blood pressure and circulating adiponectin levels in both sexes. HF or HF + HS diet impaired PVAT anticontractile effects in SMAs from females but not males. PVAT-mediated endothelial dysfunction in SMAs from female mice after 3 months of a HF + HS diet, whereas in males, this effect was observed only after 5 months of HF + HS diet. However, PVAT did not impact acetylcholine-induced relaxation in SMAs from both sexes fed HF diet. The findings suggest that the addition of sucrose to a HF diet accelerates PVAT dysfunction in both sexes. PVAT dysfunction in response to both diets was observed early in females compared to age-matched males suggesting a susceptibility of the female sex to PVAT-mediated vascular complications in the setting of obesity. The data illustrate the importance of the duration and composition of obesogenic diets for investigating sex-specific treatments and pharmacological targets for obesity-induced vascular complications.
Protective Effect of Shexiang Baoxin Pill on Myocardial Ischemia/Reperfusion Injury in Patients With STEMI
Authors: Qin Haixia , Li Siyuan , Liu Zhenbing
Published: September 2021

Background: There is no definite effect in the treatment of myocardial ischemia/reperfusion (I/R) injury in patients with acute ST-segment elevation myocardial infarction (STEMI). We evaluated the protective effect of Shexiang Baoxin Pill (SBP) on I/R injury in STEMI patients.

Methods: STEMI patients were randomly divided into a primary percutaneous coronary intervention (PPCI) group (n = 52) and a PPCI + SBP group (n = 51). The area at risk of infarction (AAR) and final infarct size (FIS) were examined by single-photon emission computed tomography (SPECT). I/R injury was assessed using myocardial salvage (MS) and salvage index (SI) calculated from AAR and FIS.

Results: The ST-segment resolution (STR) in the PPCI + SBP group was significantly higher than that in the PPCI group (p = 0.036), and the peak value of high-sensitivity troponin T (hsTNT) was lower than that in the PPCI group (p = 0.048). FIS in the PPCI + SBP group was smaller than that in the PPCI group (p = 0.047). MS (p = 0.023) and SI (p = 0.006) in the PPCI + SBP group were larger than those in the PPCI group. The left ventricular ejection fraction (LVEF) in the PPCI + SBP group was higher than that in the PPCI group (p = 0.049), and N-terminal pro-B type natriuretic peptide (NT-proBNP) level in the PPCI + SBP group was lower than that in the PPCI group (p = 0.048).

Conclusions: SBP can alleviate I/R injury (MS and SI), decrease myocardial infarction area (peak value of hsTNT and FIS), and improve myocardial reperfusion (MBG and STR) and cardiac function (LVEF and NT-proBNP).
Polysaccharides Extracted From Panax Ginseng C.A. Mey Enhance Complement Component 4 Biosynthesis in Human Hepatocytes
Authors: Liu Shuang , Liu Fangbing , Wang Tingting , Liu Jianzeng , Hu Cheng , Sun Liwei , Wang Guan
Published: September 2021

Panaxginseng C.A. Mey (ginseng) is a classic medicinal plant which is well known for enhancing immune capacity. Polysaccharides are one of the main active components of ginseng. We isolated water-soluble ginseng polysaccharides (WGP) and analyzed the physicochemical properties of WGP including molecular weight, monosaccharide composition, and structural characteristics. WGP had minimal effect on the growth of hepatocytes. Interestingly, WGP significantly increased the mRNA and protein levels of complement component 4 (C4), one of the core components of the complement system. Promoter reporter gene assays revealed that WGP significantly enhanced activity of theC4gene promoter. Deletion analyses determined that the E-box1 and Sp1 regions play key roles in WGP-inducedC4transcription. Taken together, our results suggest that WGP promotes C4 biosynthesis through upregulation of transcription. These results provide new explanation for the intrinsic mechanism by which ginseng boosts human immune capacity.
The Effects of Age, Biological Maturation and Sex on the Development of Executive Functions in Adolescents
Authors: Laureys Felien , Middelbos Lotte , Rommers Nikki , De Waelle Silke , Coppens Eline , Mostaert Mireille , Deconinck Frederik J. A. , Lenoir Matthieu
Published: September 2021

The development of executive functions (EF) has been widely investigated and is associated with various domains of expertise, such as academic achievement and sports performance. Multiple factors are assumed to influence the development of EF, among them biological maturation. Currently the effect of biological maturation on EF performance is largely unexplored, in contrast to other domains like physical development or sports performance. Therefore, this study aimed (a) to explore the effect of chronological age on EF performance and (b) to investigate to what extent age-related changes found in EF are affected by biological maturation on both sexes. To this end, EF performance and degree of maturity, indexed by percentage of predicted adult height (%PAH), of 90 adolescents (11–16 years old, 54% males) were measured on three occasions in a time frame of 12 months. A Generalized Estimating Equation (GEE) approach was used to examine the association between chronological age and %PAH and the weighted sum scores for each EF component (i.e., inhibition, planning, working memory, shifting). All models were run separately for both sexes. The males’ results indicated that EF performance improved with age and degree of maturity on all four components. Interaction effects between age and %PAH on inhibition showed that at a younger age, males with a higher %PAH had a lower chance of performing well on inhibition, whereas at later ages, males with a higher %PAH had a higher chance to have a good inhibition performance. For working memory, it seems that there is no maturity effect at a younger age, while at later ages, a disadvantage for later maturing peers compared to on-time and earlier maturing male adolescents emerged. Females showed slightly different results. Here, age positively influenced EF performance, whereas maturity only influenced inhibition. Interaction effects emerged for working memory only, with opposite results from the males. At younger ages, females with lower %PAH values seem to be scoring higher, whereas at later ages, no maturity effect is observed. This study is one of the first to investigate the effect of biological maturation on EF performance, and shows that distinct components of EF are influenced by maturational status, although the effects are different in both sexes. Further research is warranted to unravel the implications for maturation-driven effects on EF that might significantly affect domains of human functioning like academic achievement and social development.
At-CycD2 Enhances Accumulation of Above-Ground Biomass and Recombinant Proteins in Transgenic Nicotiana benthamiana Plants
Authors: Kopertekh Lilya , Reichardt Sven
Published: September 2021

Transient expression in Nicotiana benthamiana holds great potential for recombinant protein manufacturing due to its advantages in terms of speed and yield compared to stably transformed plants. To continue improving the quantity of recombinant proteins the plant host will need to be modified at both plant and cellular levels. In attempt to increase leaf mass fraction, we transformed N. benthamiana with the At-CycD2 gene, a positive regulator of the cell cycle. Phenotypic characterization of the T1 progeny plants revealed their accelerated above-ground biomass accumulation and enhanced rate of leaf initiation. In comparison to non-transgenic control the best performing line At-CycD2-15 provided 143 and 140% higher leaf and stem biomass fractions, respectively. The leaf area enlargement of the At-CycD2-15 genotype was associated with the increase of epidermal cell number compensated by slightly reduced cell size. The production capacity of the At-CycD2-15 transgenic line was superior to that of the non-transgenic N. benthamiana. The accumulation of transiently expressed GFP and scFv-TM43-E10 proteins per unit biomass was increased by 138.5 and 156.7%, respectively, compared to the wild type. With these results we demonstrate the potential of cell cycle regulator gene At-CycD2 to modulate both plant phenotype and intracellular environment of N. benthamiana for enhanced recombinant protein yield.
Psychology Doctoral Program Experiences and Student Well-Being, Mental Health, and Optimism During the COVID-19 Pandemic
Authors: Syropoulos Stylianos , Wu Deborah J. , Burrows Brooke , Mercado Evelyn
Published: September 2021

In a sample of 916 doctoral students from 144 universities across the United States, we examined psychology graduate students’ experiences in their programs, as well as their mental health, well-being, and optimism during the onset of the COVID-19 pandemic. In a path model, we found that students’ psychological experiences in their programs (i.e., social belonging, threat, and challenge) were associated with better mental health and well-being, which in turn was associated with greater optimism about the future during the COVID-19 pandemic. These findings were also corroborated in students’ open-ended responses regarding how COVID-19 has impacted their lives. Findings varied by racial, gender, and sexual identities, as racial minorities, LGBTQ+ students, and women expressed more negative psychological experiences in their programs. We outline suggestions for graduate programs to support their graduate students, which include facilitating social connection, providing encouragement, and emphasizing students’ well-being over their productivity as the current pandemic persists.
Mindfulness Is Associated With Lower Stress and Higher Work Engagement in a Large Sample of MOOC Participants
Authors: Bartlett Larissa , Buscot Marie-Jeanne , Bindoff Aidan , Chambers Richard , Hassed Craig
Published: September 2021

Objective: This study aimed to understand the associations between mindfulness, perceived stress, and work engagement in a very large sample of English-speaking adults, from 130 different countries. It also aimed to assess participants' self-reported changes following a 6-week mindfulness massive open online course (MOOC).

Methods: Participants in the 6-week MOOC were invited to complete pre-post online surveys. Cross-sectional associations were assessed using univariate linear models, followed by structural equation models to test mediation pathways in baseline data (N = 16,697). Self-reported changes in mindfulness, stress and engagement following training were assessed using paired t-tests (n = 2,105).

Results: Each standard deviation unit increase in mindfulness was associated with a 0.52 standard deviation unit decrease in perceived stress, and with 0.06 standard deviation unit increment in work engagement. 73% of the influence of mindfulness on engagement was direct. Following the mindfulness MOOC, participants reported higher mindfulness (d = 1.16), reduced perceived stress (d = 1.00) and a small improvement in work engagement (d = 0.29).

Conclusions: Mindfulness was associated with lower perceived stress and higher work engagement in both cross-sectional and longitudinal analyses. These findings support mindfulness as a potentially protective and modifiable personal resource. The MOOC format offers a low cost, highly accessible means for extending the reach and potential benefits of mindfulness training to large numbers of people.
Adult Attachment Representations and Body Image
Authors: Bonev Nikolay , Matanova Vanya
Published: September 2021

In modern literature, the body image is interpreted as a multidimensional construct, which is considered important for both individual development and quality of life. The body image is central to the self-concept and has important consequences for mental functioning. A negative body image can result in adverse psychosocial consequences for both sexes. For a long time in the professional literature the study of socio-cultural factors on the development of the body image has prevailed. This line of research creates social constructivism, in which the earliest attachment relations are eliminated, and instead the idea is suggested that external sources have a direct influence. The present text proposes an approach to the body image as a development construct, arising and developing in the attachment relations, related to the provision of security and protection. Attachment disturbances, as well as attachment disorganization, are defined as the inability to provide security and protection. Attachment relationships in connection with the development of body image develop through the mechanisms of reflection, sensitive responses to the child’s signals and synchronous relationships. In the first months of human life, the attachment needs are first and foremost the needs of the body, which are satisfied by the responses of the primary caregiver. The topic of body image discusses attachment disorganization, the understanding of attachment trauma, and the “enactment” of the loss on the body’s territory. Trauma always involves loss. Griefs that cannot be mourned and injuries that cannot be represented seem to be central to understanding the body’s problematization.
The Role of Perceived Social Support in the Association Between Stressful Life Events and Suicidal Behavior
Authors: Panesar Balpreet , Rosic Tea , Rodrigues Myanca , Sanger Nitika , Baptist-Mohseni Natasha , Hillmer Alannah , Chawar Caroul , D'Elia Alessia , Minuzzi Luciano , Thabane Lehana , Samaan Zainab
Published: September 2021

Background: Suicide is a serious public health concern for which there have been well-established protective and risk factors reported in literature. There is a lack of evidence on the indirect effects of other variables on these factors. Specifically, the association between stressful life events and suicidal behavior may be affected by perceived social support, but its role in this association is largely uninvestigated.

Objectives: Thus, this paper aims to explore the role of perceived social support in the association between stressful life events and suicidal behavior. Perceived social support will be explored as a mediator and as a moderator in this association.

Methods: Data were obtained from the Determinants of Suicidal Behavior Conventional and Emergent Risk (DISCOVER), a study conducted to identify risk factors of suicidal behavior. The study participants are individuals with suicide attempts admitted to hospital. Participants (n = 343) were recruited from hospital setting. Suicidal behavior was measured using two outcomes (1) the occurrence of a suicide attempt (2) level of suicide intent as measured by the Pierce Suicide Intent Scale. Perceived social support was measured using the Sarason Social Support Questionnaire.

Results: Stressful life events were significantly associated with suicide attempts (OR 1.440, 95% CI 1.440, 1.682, p < 0.001) and perceived social support (B −0.785, 95% CI −1.501, −0.068, p = 0.032). There was no significant mediation effect by perceived social support in the association between stressful life events and suicide attempts (Sobel's test statistic 1.64, p = 0.100). Perceived social support did not moderate the relationship between stressful life events and suicide attempts [(OR 1.007, 95% CI 0.987, 1.027, p = 0.514] or the relationship between stressful life events and level of suicidal intent (B −0.043, 95% CI −0.132, 0.046, p = 0.343).

Conclusion: Stressful life events are associated with increased risk of suicide attempts. The study also identified an inverse relationship between stressful life events and perceived social support. These associations were independent of perceived social support. This study highlights the effects of stressful life events on suicide risk is not affected by perceived social support, requiring further investigation into measures to reduce the impact of social stressors on people with risk of suicide.
Anabolic Function Downstream of TOR Controls Trade-offs Between Longevity and Reproduction at the Level of Specific Tissues in C. elegans
Authors: Howard Amber C. , Mir Dilawar , Snow Santina , Horrocks Jordan , Sayed Hussein , Ma Zhengxin , Rogers Aric N.
Published: September 2021

As the most energetically expensive cellular process, translation must be finely tuned to environmental conditions. Dietary restriction attenuates signaling through the nutrient sensing mTOR pathway, which reduces translation and redirects resources to preserve the soma. These responses are associated with increased lifespan but also anabolic impairment, phenotypes also observed when translation is genetically suppressed. Here, we restricted translation downstream of mTOR separately in major tissues in C. elegans to better understand their roles in systemic adaptation and whether consequences to anabolic impairment were separable from positive effects on lifespan. Lowering translation in neurons, hypodermis, or germline tissue led to increased lifespan under well-fed conditions and improved survival upon withdrawal of food, indicating that these are key tissues coordinating enhanced survival when protein synthesis is reduced. Surprisingly, lowering translation in body muscle during development shortened lifespan while accelerating and increasing reproduction, a reversal of phenotypic trade-offs associated with systemic translation suppression. Suppressing mTORC1 selectively in body muscle also increased reproduction while slowing motility during development. In nature, this may be indicative of reduced energy expenditure related to foraging, acting as a “GO!” signal for reproduction. Together, results indicate that low translation in different tissues helps direct distinct systemic adaptations and suggest that unknown endocrine signals mediate these responses. Furthermore, mTOR or translation inhibitory therapeutics that target specific tissues may achieve desired interventions to aging without loss of whole-body anabolism.
Fire Hazard in Chattogram City Corporation Area: A Critical Analysis of Its Causes and Mitigation Measures
Authors: Hasan Muhammad Rashidul , Kauser Md. Rakibul Hasan , Ibrahim Jihan
Published: September 2021

Fire can have its origin either in natural or human activities resulting in death and/or property damage in a very short period of time. To develop fire hazard-resilient cities proper attention should be given to all possible sections of development to facilitate the service. Chattogram City is the second largest city of Bangladesh. Due to rapid rate of urbanization and unplanned growth of urban centers; hazards like fire have become a menace for the Chattogram City Corporation (CCC) area. So, the main objective of this research is to identify the nature of the fire hazard by analyzing frequency and intensity over time through different checklists and analyzing the existing firefighting capacity. In addition to past fire incident records, a direct questionnaire survey was utilized and expert opinion was solicited for primary data collection. Using geographical information systems (GIS) techniques, this study found that nine fire stations in the CCC area exhibited different frequency and intensity in different categories such as type of structure, land use, source of fire, damage, and losses in last 5 years. The number of fire incidents increased from 200 to 675 which indicates that the frequency increased over time. Among 2,514 fire accidents, 47% occurred in residential areas and 27% occurred in commercial areas. Fire intensity also varied with seasonal variation over a year. Within the last 5 years, the Chattogram City Corporation dealt with a 179,091,200 BDT financial loss and 83 people were injured, of which 15 were due to fire accidents. One the other hand, only nine fire stations serve the ~155 sq.km area and ~6 million people live in the CCC area which is 17 and 19 times larger than that of Bangkok and Oklahoma City. Finally, this data-driven approach and findings could help emergency planners to take necessary steps for improving firefighting capability to ensure better fire safety and resiliency.
Revisión sistemática sobre la educación ambiental universitaria en Latinoamérica durante la pandemia (2020-2021)
Authors: Armesto Céspedes Miguel Sebastián , Vallejos Armas Rony Isaac
Published: September 2021

En los últimos 50 años, el cuidado del medio ambiente se ha consolidado en la agenda global y regional; y dentro de ella, la educación ambiental (EA) ha sido uno de los pilares, capaz de re direccionar y modificar el comportamiento de los ciudadanos en pro del equilibrio hombre-naturaleza. No obstante, con el desarrollo de la pandemia por COVID-19 y las declaratorias de aislamiento social obligatorio en todas las regiones, se han tenido que replantear las metas programadas. En esta línea, el objetivo del presente trabajo fue sistematizar las experiencias y evidencias respecto a la educación ambiental en las universidades en Latinoamérica durante este contexto. Para ello fueron analizados 11 artículos científicos en español de las siguientes bases de datos: Scopus, Ebsco, Proquest, Scielo y Dialnet. Para dicho análisis, se tuvo en cuenta criterios de inclusión y exclusión; se trabajó con los artículos publicados en español entre el 2020 y 2021. Entre las conclusiones se obtuvo que en Latinoamérica la EA ha sido objeto de investigaciones en los que se priorizó el diagnóstico para sus posteriores planes de mejora e implementación.