bluuebunny/crossref_metadata_2025 huggingface dataset:
ADCY5, CAPN10 and JAZF1 Gene Polymorphisms and Placental Expression in Women with Gestational Diabetes
Authors: Ustianowski Przemysław , Malinowski Damian , Kopytko Patrycja , Czerewaty Michał , Tarnowski Maciej , Dziedziejko Violetta , Safranow Krzysztof , Pawlik Andrzej
Published: August 2021

Gestational diabetes mellitus (GDM) is carbohydrate intolerance that occurs during pregnancy. This disease may lead to various maternal and neonatal complications; therefore, early diagnosis is very important. Because of the similarity in pathogenesis of type 2 diabetes and GDM, the genetic variants associated with type 2 diabetes are commonly investigated in GDM. The aim of the present study was to examine the associations between the polymorphisms in the ADCY5 (rs11708067, rs2877716), CAPN10 (rs2975760, rs3792267), and JAZF1 (rs864745) genes and GDM as well as to determine the expression of these genes in the placenta. This study included 272 pregnant women with GDM and 348 pregnant women with normal glucose tolerance. The diagnosis of GDM was based on a 75 g oral glucose tolerance test (OGTT) at 24–28 weeks gestation, according to International Association of Diabetes and Pregnancy Study Groups (IADPSG) criteria. There were no statistically significant differences in the distribution of the ADCY5 gene (rs11708067, rs2877716) and CAPN10 gene (rs2975760, rs3792267) polymorphisms between pregnant women with normal carbohydrate tolerance and pregnant women with GDM. We have shown a lower frequency of JAZF1 gene rs864745 C allele carriers among women with GDM CC + CT vs. TT (OR = 0.60, 95% CI = 0.41–0.87, p = 0.006), and C vs. T (OR = 0.75, 95% CI = 0.60–0.95, p = 0.014). In addition, ADCY5 and JAZF1 gene expression was statistically significantly increased in the placentas of women with GDM compared with that of healthy women. The expression of the CAPN10 gene did not differ significantly between women with and without GDM. Our results indicate increased expression of JAZF1 and ADCY5 genes in the placentas of women with GDM as well as a protective effect of the C allele of the JAZF1 rs864745 gene polymorphism on the development of GDM in pregnant women.
Nemesignis, a Replacement Name for Nemesis Furfaro & Mariottini, 2021 (Mollusca, Gastropoda, Myrrhinidae), Preoccupied by Nemesis Risso, 1826 (Crustacea, Copepoda)
Authors: Furfaro Giulia , Mariottini Paolo
Published: August 2021

The genus Nemesis Furfaro & Mariottini, 2021, was recently introduced for an independent lineage of aeolid nudibranchs, and Dondice banyulensis Portmann & Sandmeier, 1960, established as its type species. Anyway, the presence of a senior homonym, Nemesis Risso, 1826, was evidently missed. In fact, in 1826, Risso established this genus for a group of Copepoda (Arthropoda, Crustacea) and according to the Principle of Priority (ICZN) only the senior homonym may be used as a valid name. Therefore, a new replacement name is here proposed. Furthermore, the genus name Nanuca Er. Marcus, 1957, has priority over Dondice Er. Marcus, 1958 and consequently, the species in this clade should be classified under Nanuca, mostly as new combinations.
Preliminary Animal Study on Bone Formation Ability of Commercialized Particle-Type Bone Graft with Increased Operability by Hydrogel
Authors: Kim So-Yeun , Lee You-Jin , Cho Won-Tak , Hwang Su-Hyun , Heo Soon-Chul , Kim Hyung-Joon , Huh Jung-Bo
Published: August 2021

The purpose of this study was to evaluate the bone-generating ability of a new bovine-derived xenograft (S1-XB) containing hydrogel. For control purposes, we used Bio-Oss and Bone-XB bovine-derived xenografts. S1-XB was produced by mixing Bone-XB and hydrogel. Cell proliferation and differentiation studies were performed to assess cytotoxicities and cell responses. For in vivo study, 8 mm-sized cranial defects were formed in 16 rats, and then the bone substitutes were transplanted into defect sites in the four study groups, that is, a Bio-Oss group, a Bone-XB group, an S1-XB group, and a control (all n = 4); in the control group defects were left empty. Eight weeks after surgery, new bone formation areas were measured histomorphometrically. In the cell study, extracts of Bio-Oss, Bone-XB, and S1-XB showed good results in terms of the osteogenic differentiation of human mesenchymal stem cells (hMSCs) and no cytotoxic reaction was evident. No significant difference was observed between mean new bone areas in the Bio-Oss (36.93 ± 4.27%), Bone-XB (35.07 ± 3.23%), and S1-XB (30.80 ± 6.41%) groups, but new bone area was significantly smaller in the control group (18.73 ± 5.59%) (p < 0.05). Bovine-derived bone graft material containing hydrogel (S1-XB) had a better cellular response and an osteogenic effect similar to Bio-Oss.
Studies of New Iridium Catalysts Supported on Modified Silicalite-1—Their Structure and Hydrogenating Properties
Authors: Zieliński Michał , Kot Monika , Pietrowski Mariusz , Wojcieszak Robert , Kowalska-Kuś Jolanta , Janiszewska Ewa
Published: August 2021

This paper investigates the catalytic properties of the iridium catalysts supported on modified silicalite-1. Post-synthesis modification of silicalite-1, with solutions of ammonium compounds (NH4F and NH4OH), appeared to be an efficient method to generate the acidic sites in starting support. The modification of support led not only to changes in its acidity but also its porosity—formation of additional micro- and mesopores. The novel materials were used as supports for iridium. The iridium catalysts (1 wt.% Ir) were characterized by N2 adsorption/desorption measurements, temperature-programmed reduction with hydrogen (TPR-H2), H2 chemisorption, transmission electron microscopy (TEM), temperature-programmed desorption of ammonia (TPD-NH3), X-ray photoelectron spectroscopy (XPS) and tested in the hydrogenation of toluene reaction. The catalytic activity of iridium supported on silicalite-1 treated with NH4OH (higher porosity of support, better dispersion of active phase) was much higher than that of Ir supported on unmodified and modified with NH4F silicalite-1.
SEM Evaluation of Tooth Surface after a Composite Filling Removal Using Er:YAG Laser, Drills with and without Curettes, and Optional EDTA or NaOCl Conditioning
Authors: Kiryk Jan , Matys Jacek , Grzech-Leśniak Kinga , Dominiak Marzena , Małecka Małgorzata , Kuropka Piotr , Wiglusz Rafał J. , Dobrzyński Maciej
Published: August 2021

(1) Background: This study aimed to evaluate the microporosity of the tooth surface structure adjacent to the cemento-enamel junction (CEJ) after the removal of composite fillings with a drill in comparison with removal by an Er:YAG laser and after cleaning with a periodontal curette, chemical EDTA and NaOCl (sodium hypochlorite) conditioning. (2) Methods: The research material consisted of 30 extracted premolars with cervical composite fillings. The teeth were divided into six groups according to the method of tooth preparation: group G1 (n = 5)—a diamond drill; group G2 (n = 5)—a diamond drill + curette; group G3 (n = 5)—a diamond drill + 24% EDTA (PrefGel, Straumann, Switzerland); group G4 (n = 5)—an Er:YAG laser (LightWalker, Fotona, Ljubljana, Slovenia) set with the following parameters: power: 1.65 W (composite removal, CR), 1.2 (tooth conditioning, TC), energy: 110 mJ (CR), 80 mJ (TC), frequency: 15 Hz, pulse duration: 50 μs, tip diameter: 1 mm, air/fluid cooling: 4, distance 1.5 mm, energy density: 14.01 J/cm2 (CR), 10.19 J/cm2 (TC); group G5 (n = 5)—an Er:YAG laser + 2% sodium hypochlorite (NaOCl); group G6 (n = 5)—an Er:YAG laser + 5.25% NaOCl. In each tooth, three cavities were made and subjected to analysis. The dentin surface was evaluated using a scanning electron microscope (SEM). (3) Results: Groups G1 and G2 exhibited mechanical damage to the tooth surface structure caused by the rotary motion of a diamond drill. The SEM image showed a smear layer that could only be removed chemically using 24% EDTA gel (group G3). The tooth surfaces prepared with the Er:YAG laser (groups G4–G6) revealed a homogeneous structure without damage along with open dentinal tubules (without smear layer) and visible denaturation of collagen fibers. The sodium hypochlorite (NaOCl) conditioning did not increase the visibility of dentinal tubules. (4) Conclusions: Dentin surfaces have open dentinal tubules after removal of the composite filling using the Er:YAG laser and therefore do not require additional NaOCl conditioning.
Texturing and Phase Evolution in Ti-6Al-4V: Effect of Electron Beam Melting Process, Powder Re-Using, and HIP Treatment
Authors: Popov Vladimir V. , Lobanov Mikhail L. , Stepanov Stepan I. , Qi Yuanshen , Muller-Kamskii Gary , Popova Elena N. , Katz-Demyanetz Alexander , Popov Artemiy A.
Published: August 2021

The research demonstrates microstructural changes and development of specific texture in Ti-6Al-4V specimens produced by electron beam melting (EBM) under different conditions. The effect of two factors, namely, raw material (powder) recycling and hot isostatic pressing (HIP), on the EBM produced samples structure and properties, has been explored. The as-printed and treated samples were investigated using electron backscattered diffraction (EBSD) analysis. Modification of mechanical properties after the EBM and HIP are explained by the EBSD data on microstructural phenomena and phase transformations. The work is devoted to assessing the possibility of reusing the residual titanium alloy powder for the manufacture of titanium components by the combination of EBM and HIP methods.
Development of 3D Printable Cementitious Composites with the Incorporation of Polypropylene Fibers
Authors: Van Der Putten Jolien , Rahul Attupurathu Vijayan , De Schutter Geert , Van Tittelboom Kim
Published: August 2021

Similar to conventional cast concrete, printable materials require reinforcement to counteract their low tensile strength. However, as traditional reinforcement strategies are not commonly used in 3D print applications, fiber reinforcement can serve as an alternative. This study aims to assess the influence of different polypropylene fiber lengths (3 and 6 mm, denoted as M3 and M6, respectively) and dosages (0.1 and 0.3% volume fraction) on the workability, pore structure, mechanical and shrinkage behavior of 3D printable cementitious materials. Fresh state observations revealed that the addition of a higher fiber volume decreased the workability of the material, irrespective of the fiber length as a result of the lower water film thickness (WFT). In hardened state, a marginal increase in total porosity could be observed when adding fibers to the mix composition. In addition, the flexural strength was found to increase with the addition of fibers, while no significant difference was observed in compressive strength. The increase in flexural strength was more pronounced in the case of longer-sized M6 fibers. Finally, the total drying shrinkage behavior was evaluated using mold-cast prisms. The addition of M6 fibers showed no beneficial effect in reducing total free shrinkage, while a reduction in total free shrinkage was observed when using M3 fibers.
Enhancement of Mechanical Properties of Pure Aluminium through Contactless Melt Sonicating Treatment
Authors: Dybalska Agnieszka , Caden Adrian , Griffiths William D. , Nashwan Zakareya , Bojarevics Valdis , Djambazov Georgi , Tonry Catherine E. H. , Pericleous Koulis A.
Published: August 2021

A new contactless ultrasonic sonotrode method was previously designed to provide cavitation conditions inside liquid metal. The oscillation of entrapped gas bubbles followed by their final collapse causes extreme pressure changes leading to de-agglomeration and the dispersion of oxide films. The forced wetting of particle surfaces and degassing are other mechanisms that are considered to be involved. Previous publications showed a significant decrease in grain size using this technique. In this paper, the authors extend this research to strength measurements and demonstrate an improvement in cast quality. Degassing effects are also interpreted to illustrate the main mechanisms involved in alloy strengthening. The mean values and Weibull analysis are presented where appropriate to complete the data. The test results on cast Al demonstrated a maximum of 48% grain refinement, a 28% increase in elongation compared to 16% for untreated material and up to 17% increase in ultimate tensile strength (UTS). Under conditions promoting degassing, the hydrogen content was reduced by 0.1 cm3/100 g.
Application of Shape Memory Alloys in Retrofitting of Masonry and Heritage Structures Based on Their Vulnerability Revealed in the Bam 2003 Earthquake
Authors: Tabrizikahou Alireza , Hadzima-Nyarko Marijana , Kuczma Mieczysław , Lozančić Silva
Published: August 2021

For decades, one of the most critical considerations of civil engineers has been the construction of structures that can sufficiently resist earthquakes. However, in many parts of the globe, ancient and contemporary buildings were constructed without regard for engineering; thus, there is a rising necessity to adapt existing structures to avoid accidents and preserve historical artefacts. There are various techniques for retrofitting a masonry structure, including foundation isolations, the use of Fibre-Reinforced Plastics (FRPs), shotcrete, etc. One innovative technique is the use of Shape Memory Alloys (SMAs), which improve structures by exhibiting high strength, good re-centring capabilities, self-repair, etc. One recent disastrous earthquake that happened in the city of Bam, Iran, (with a large proportion of masonry buildings) in 2003, with over 45,000 casualties, is analysed to discover the primary causes of the structural failure of buildings and its ancient citadel. It is followed by introducing the basic properties of SMAs and their applications in retrofitting masonry buildings. The outcomes of preceding implementations of SMAs in retrofitting of masonry buildings are then employed to present two comprehensive schemes as well as an implementation algorithm for strengthening masonry structures using SMA-based devices.
Technical Challenges of Utilizing Ground Tire Rubber in Asphalt Pavements in the United States
Authors: Ghabchi Rouzbeh , Arshadi Amir , Zaman Musharraf , March Ferrella
Published: August 2021

At least 275 million scrap tires exist in stockpiles in the U.S. The practice of dumping scrap tires in landfills has been an environmental concern. To address this concern, many industries—and regional and national environmental protection agencies—have taken major initiatives to recycle scrap tires. One of the major uses of recycled scrap tires is in crumb rubber products, including rubberized asphalt. Rubberized asphalt is produced by blending ground tire rubber with asphalt to beneficially modify its properties for highway construction. The ground tire rubber (GTR) can be used either as part of the asphalt rubber binder (also known as asphalt rubber), seal coat, cap seal spray, joint and crack sealant or as substitute aggregate (rubber-modified asphalt concrete). Therefore, the largest single market for GTR is asphalt rubber, which consumes approximately 12 million tires, annually. Currently, several Departments of Transportation (DOTs) in the U.S. do not allow use of GTR in asphalt mixes. This is partly due to lack of information, laboratory test data and specifications or special provisions on the use of GTR in asphalt pavements. The current study was undertaken to summarize the available wealth of knowledge, identify research needs, and document the major findings of previous pertinent studies focused on GTR use in asphalt. Significant study findings—consisting of laboratory test results, field observations, and common practices—were documented, including: the use of GTR in asphalt mixes, wet and dry processes, characterization of hot mix asphalt (HMA) containing GTR and GTR performance when combined with virgin materials. In order to promote successful use of GTR, it is imperative to help DOTs develop specifications/special provisions for utilizing rubberized asphalt by collecting data, common practices and specifications utilized by other state DOTs. As a part of this effort, we conducted a survey of construction specifications used by different DOTs that currently allow the use of GTR in asphalt. Since some DOT practices are not readily available in the open literature, this survey proved to be an effective tool for gathering data on the current practices, methods and specifications associated with DOT use of GTR in asphalt pavement.
An Extension of the Truncated-Exponential Skew- Normal Distribution
Authors: Rivera Pilar A. , Gallardo Diego I. , Venegas Osvaldo , Bourguignon Marcelo , Gómez Héctor W.
Published: August 2021

In the paper, we present an extension of the truncated-exponential skew-normal (TESN) distribution. This distribution is defined as the quotient of two independent random variables whose distributions are the TESN distribution and the beta distribution with shape parameters q and 1, respectively. The resulting distribution has a more flexible coefficient of kurtosis. We studied the general probability density function (pdf) of this distribution, its survival and hazard functions, some of its properties, moments and inference by the maximum likelihood method. We carried out a simulation and applied the methodology to a real dataset.
A Link between Approximation Theory and Summability Methods via Four-Dimensional Infinite Matrices
Authors: Srivastava Hari M. , Ansari Khursheed J. , Özger Faruk , Ödemiş Özger Zeynep
Published: August 2021

In this study, we present a link between approximation theory and summability methods by constructing bivariate Bernstein-Kantorovich type operators on an extended domain with reparametrized knots. We use a statistical convergence type and power series method to obtain certain Korovkin type theorems, and we study certain rates of convergences related to these summability methods. Furthermore, we numerically analyze the theoretical results and provide some computer graphics to emphasize the importance of this study.
An Algorithm for Linearizing the Collatz Convergence
Authors: Rahn Alexander , Sultanow Eldar , Henkel Max , Ghosh Sourangshu , Aberkane Idriss
Published: August 2021

The Collatz dynamic is known to generate a complex quiver of sequences over natural numbers for which the inflation propensity remains so unpredictable it could be used to generate reliable proof-of-work algorithms for the cryptocurrency industry; it has so far resisted every attempt at linearizing its behavior. Here, we establish an ad hoc equivalent of modular arithmetics for Collatz sequences based on five arithmetic rules that we prove apply to the entire Collatz dynamical system and for which the iterations exactly define the full basin of attractions leading to any odd number. We further simulate these rules to gain insight into their quiver geometry and computational properties and observe that they linearize the proof of convergence of the full rows of the binary tree over odd numbers in their natural order, a result which, along with the full description of the basin of any odd number, has never been achieved before. We then provide two theoretical programs to explain why the five rules linearize Collatz convergence, one specifically dependent upon the Axiom of Choice and one on Peano arithmetic.
A Remark on the Change of Variable Theorem for the Riemann Integral
Authors: Kuleshov Alexander
Published: August 2021

In 1961, Kestelman first proved the change in the variable theorem for the Riemann integral in its modern form. In 1970, Preiss and Uher supplemented his result with the inverse statement. Later, in a number of papers (Sarkhel, Výborný, Puoso, Tandra, and Torchinsky), the alternative proofs of these theorems were given within the same formulations. In this note, we show that one of the restrictions (namely, the boundedness of the function f on its entire domain) can be omitted while the change of variable formula still holds.
Mesh Free Radial Point Interpolation Based Displacement Recovery Techniques for Elastic Finite Element Analysis
Authors: Ahmed Mohd. , Singh Devinder , AlQadhi Saeed , Alrefae Majed A.
Published: August 2021

The study develops the displacement error recovery method in a mesh free environment for the finite element solution employing the radial point interpolation (RPI) technique. The RPI technique uses the radial basis functions (RBF), along with polynomials basis functions to interpolate the displacement fields in a node patch and recovers the error in displacement field. The global and local errors are quantified in both energy and L2 norms from the post-processed displacement field. The RPI technique considers multi-quadrics/gaussian/thin plate splint RBF in combination with linear basis function for displacement error recovery analysis. The elastic plate examples are analyzed to demonstrate the error convergence and effectivity of the RPI displacement recovery procedures employing mesh free and mesh dependent patches. The performance of a RPI-based error estimators is also compared with the mesh dependent least square based error estimator. The triangular and quadrilateral elements are used for the discretization of plates domains. It is verified that RBF with their shape parameters, choice of elements, and errors norms influence considerably on the RPI-based displacement error recovery of finite element solution. The numerical results show that the mesh free RPI-based displacement recovery technique is more effective and achieve target accuracy in adaptive analysis with the smaller number of elements as compared to mesh dependent RPI and mesh dependent least square. It is also concluded that proposed mesh free recovery technique may prove to be most suitable for error recovery and adaptive analysis of problems dealing with large domain changes and domain discontinuities.
A Method for Rapid Prediction of Edge Defects in Cold Roll Forming Process
Authors: Meng Zhijuan , Fang Yanan , Ma Lidong
Published: August 2021

In order to implement rapid prediction of edge defects in the cold roll forming process, a new analytical method based on the mean longitudinal strain of the racks is presented. A cubic spline curve with the parameters of the cumulative chord length is applied to fit the corresponding points and center points of different passes, and fitting curves are obtained. As the cold roll forming is micro-tension forming, the tensions between racks are ignored. Then the mean longitudinal strains between racks are obtained. By comparing the mean longitudinal strain between racks and the yield strain of the material, we can judge whether there are defects at the edges. Finally, the reasonableness of this method is illustrated and validated by an example. With this method, the roll forming effect can be quickly predicted, and the position where a greater longitudinal strain occurred can be determined. In order to prevent the defects, the deformation angles need to be modified when the result is beyond the yield strain. To further prove the correctness of the theory, the results of the analytical method are compared with the ones of the non-linear finite element software ABAQUS. The analytical results have the same trend as the finite element results. This method can provide useful guidance to the actual design process.
Integral Equations Related to Volterra Series and Inverse Problems: Elements of Theory and Applications in Heat Power Engineering
Authors: Solodusha Svetlana , Bulatov Mikhail
Published: August 2021

The paper considers two types of Volterra integral equations of the first kind, arising in the study of inverse problems of the dynamics of controlled heat power systems. The main focus of the work is aimed at studying the specifics of the classes of Volterra equations of the first kind that arise when describing nonlinear dynamics using the apparatus of Volterra integro-power series. The subject area of the research is represented by a simulation model of a heat exchange unit element, which describes the change in enthalpy with arbitrary changes in fluid flow and heat supply. The numerical results of solving the problem of identification of transient characteristics are presented. They illustrate the fundamental importance of practical recommendations based on sufficient conditions for the solvability of linear multidimensional Volterra equations of the first kind. A new class of nonlinear systems of integro-algebraic equations of the first kind, related to the problem of automatic control of technical objects with vector inputs and outputs, is distinguished. For such systems, sufficient conditions are given for the existence of a unique, sufficiently smooth solution. A review of the literature on these problem types is given.
A Simple Predictive Marker in Cardiac Resynchronization Therapy Recipients: Prominent S-Wave in Right Precordial Leads
Authors: Kataoka Naoya , Imamura Teruhiko , Koi Takahisa , Uchida Keisuke , Kinugawa Koichiro
Published: August 2021

Background and objectives: Current guidelines criteria do not satisfactorily discriminate responders to cardiac resynchronization therapy (CRT). QRS amplitude is an established index to recognize the severity of myocardial disturbance and might be a key to optimal patient selection for CRT. Materials and Methods: (1) Initial R-wave amplitude, (2) S-wave amplitude, and (3) a summation of maximal R- or R′-wave amplitude and S-wave amplitude were measured at baseline. These parameters were averaged according to right (V1 to V3) or left (V4 to V6) precordial leads. The impact of these parameters on response to CRT, which was defined as a decrease in left ventricular end-systolic volume ≥15% at six-month follow-up, was investigated. Results: Among 47 patients (71 years old, 28 men) who received guideline-indicated CRT implantation, 25 (53%) achieved the definition of CRT responder. Among baseline electrocardiogram parameters, only the higher S-wave amplitude in right precordial leads was an independent predictor of CRT responders (odds ratio: 2.181, 95% confidence interval: 1.078–4.414, p = 0.030) at a cutoff of 1.44 mV. The cutoff was independently associated with cumulative incidence of heart failure readmission and appropriate electrical defibrillation following CRT implantation (p < 0.05, respectively). Conclusions: Prominent S-wave in right precordial leads might be a promising index to predict left ventricular reverse remodeling and greater clinical outcomes following CRT implantation.
Physicochemical Properties of Mesoporous Organo-Silica Xerogels Fabricated through Organo Catalyst
Authors: Elma Muthia , Sumardi Anna , Paramita Adhe , Rahma Aulia , Lestari Aptar Eka , Yanto Dede Heri Yuli , Hadi Sutarto , Assyaifi Zaini Lambri , Sunardi  , Raharjo Yanuardi
Published: August 2021

The physicochemical properties of organo-silica xerogels derived from organo catalyst were pervasively investigated, including the effect of one-step catalyst (citric acid) and two-step catalyst (acid-base), and also to observe the effect of sol pH of organo-silica xerogel toward the structure and deconvolution characteristic. The organo-silica xerogels were characterized by FTIR, TGA and nitrogen sorption to obtain the physicochemical properties. The silica sol–gel method was applied to processed materials by employing TEOS (tetraethyl orthosilicate) as the main precursor. The final molar ratio of organo-silica was 1:38:x:y:5 (TEOS:ethanol: citric acid: NH3:H2O) where x is citric acid concentration (0.1–10 × 10−2 M) and y is ammonia concentration (0 to 3 × 10−3 M). FTIR spectra shows that the one-step catalyst xerogel using citric acid was handing over the higher Si-O-Si concentration as well as Si-C bonding than the dual catalyst xerogels with the presence of a base catalyst. The results exhibited that the highest relative area ratio of silanol/siloxane were 0.2972 and 0.1262 for organo catalyst loading at pH 6 and 6.5 of organo-silica sols, respectively. On the other hand, the organo-silica matrices in this work showed high surface area 546 m2 g−1 pH 6.5 (0.07 × 10−2 N citric acid) with pore size ~2.9 nm. It is concluded that the xerogels have mesoporous structures, which are effective for further application to separate NaCl in water desalination.
Subsurface Microstructural Evolution during Scratch Testing on Bcc Iron
Authors: Linsler Dominic , Ruebeling Friederike , Greiner Christian
Published: August 2021

Subsurface microstructures influence the friction and wear behavior of metallic tribological systems, among other factors. To gain a basic understanding of the microstructural changes occurring during sliding processes, face-centered cubic model systems, for example a copper system with a sapphire sphere sliding against it, were previously characterized. Such systems showed the evolution of the dislocation self-organization phenomenon called the dislocation trace line. To test the occurrence of this dislocation arrangement in bcc metals, in this study a ruby ball was slid against electropolished bcc iron under an increasing normal load. The wear track topography and subsurface microstructure were characterized using white light interferometry and scanning transmission electron microscopy. The analysis suggested that at least for bcc iron, the evolution of a dislocation trace line is connected with the onset of pronounced plastic deformation.
The Role of Gut Microbiota and Its Produced Metabolites in Obesity, Dyslipidemia, Adipocyte Dysfunction, and Its Interventions
Authors: Zwartjes Max S. Z. , Gerdes Victor E. A. , Nieuwdorp Max
Published: August 2021

Obesity is becoming an increasing problem worldwide and is often, but not invariably, associated with dyslipidemia. The gut microbiota is increasingly linked to cardiovascular disease, nonalcoholic fatty liver disease, and type 2 diabetes mellitus. However, relatively little focus has been attributed to the role of gut-microbiota-derived metabolites in the development of dyslipidemia and alterations in lipid metabolism. In this review, we discuss current data involved in these processes and point out the therapeutic potentials. We cover the ability of gut microbiota metabolites to alter lipoprotein lipase action, VLDL secretion, and plasma triglyceride levels, and its effects on reverse cholesterol transport, adipocyte dysfunction, and adipose tissue inflammation. Finally, the current intervention strategies for treatment of obesity and dyslipidemia is addressed with emphasis on the role of gut microbiota metabolites and its ability to predict treatment efficacies.
A Digitized Representation of the Modified Prandtl–Ishlinskii Hysteresis Model for Modeling and Compensating Piezoelectric Actuator Hysteresis
Authors: Zhou Chao , Feng Chen , Aye Yan Naing , Ang Wei Tech
Published: August 2021

Piezoelectric actuators are widely used in micromanipulation and miniature robots due to their rapid response and high repeatability. The piezoelectric actuators often have undesired hysteresis. The Prandtl–Ishlinskii (PI) hysteresis model is one of the most popular models for modeling and compensating the hysteresis behaviour. This paper presents an alternative digitized representation of the modified Prandtl–Ishlinskii with the dead-zone operators (MPI) hysteresis model to describe the asymmetric hysteresis behavior of piezoelectric actuators. Using a binary number with n digits to represent the classical Prandtl–Ishlinskii hysteresis model with n elementary operators, the inverse model can be easily constructed. A similar representation of the dead-zone operators is also described. With the proposed digitized representation, the model is more intuitive and the inversion calculation is avoided. An experiment with a piezoelectric stacked linear actuator is conducted to validate the proposed digitized MPI hysteresis model and it is shown that it has almost the same performance as compared to the classical representation.
Molecular Epidemiology of SARS-CoV-2 in Diverse Environmental Samples Globally
Authors: Islam Ariful , Sayeed Md. Abu , Kalam Md. Abul , Ferdous Jinnat , Rahman Md. Kaisar , Abedin Josefina , Islam Shariful , Shano Shahanaj , Saha Otun , Shirin Tahmina , Hassan Mohammad Mahmudul
Published: August 2021

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has swamped the global environment greatly in the current pandemic. Wastewater-based epidemiology (WBE) effectively forecasts the surge of COVID-19 cases in humans in a particular region. To understand the genomic characteristics/footprints and diversity of SARS-CoV-2 in the environment, we analyzed 807 SARS-CoV-2 sequences from 20 countries deposited in GISAID till 22 May 2021. The highest number of sequences (n = 638) were reported in Austria, followed by the Netherlands, China, and Bangladesh. Wastewater samples were highest (40.0%) to successfully yield the virus genome followed by a 24 h composite wastewater sample (32.6%) and sewage (18.5%). Phylogenetic analysis revealed that SARS-CoV-2 environmental strains are a close congener with the strains mostly circulating in the human population from the same region. Clade GRY (32.7%), G (29.2%), GR (25.3%), O (7.2%), GH (3.4%), GV (1.4%), S (0.5%), and L (0.4%) were found in environmental samples. Various lineages were identified in environmental samples; nevertheless, the highest percentages (49.4%) of the alpha variant (B.1.1.7) were detected in Austria, Liechtenstein, Slovenia, Czech Republic, Switzerland, Germany, and Italy. Other prevalent lineages were B.1 (18.2%), B.1.1 (9.2%), and B.1.160 (3.9%). Furthermore, a significant number of amino acid substitutions were found in environmental strains where the D614G was found in 83.8% of the sequences. However, the key mutations—N501Y (44.6%), S982A (44.4%), A570D (43.3%), T716I (40.4%), and P681H (40.1%) were also recorded in spike protein. The identification of the environmental belvedere of SARS-CoV-2 and its genetic signature is crucial to detect outbreaks, forecast pandemic harshness, and prepare with the appropriate tools to control any impending pandemic. We recommend genomic environmental surveillance to trace the emerging variants and diversity of SARS-CoV-2 viruses circulating in the community. Additionally, proper disposal and treatment of wastewater, sewage, and medical wastes are important to prevent environmental contamination.
Repurposing of the Fasciolicide Triclabendazole to Treat Infections Caused by Staphylococcus spp. and Vancomycin-Resistant Enterococci
Authors: Pi Hongfei , Ogunniyi Abiodun D. , Savaliya Bhumi , Nguyen Hang Thi , Page Stephen W. , Lacey Ernest , Venter Henrietta , Trott Darren J.
Published: August 2021

One approach to combat the increasing incidence of multidrug-resistant (MDR) bacterial pathogens involves repurposing existing compounds with known safety and development pathways as new antibacterial classes with potentially novel mechanisms of action. Here, triclabendazole (TCBZ), a drug originally developed to treat Fasciola hepatica (liver fluke) in sheep and cattle, and later in humans, was evaluated as an antibacterial alone or in combination with sub-inhibitory concentrations of polymyxin B (PMB) against clinical isolates and reference strains of key Gram-positive and Gram-negative bacteria. We show for the first time that in vitro, TCBZ selectively kills methicillin-sensitive and methicillin-resistant Staphylococcus aureus and Staphylococcus pseudintermedius at a minimum inhibitory concentration (MIC) range of 2–4 µg/mL, and vancomycin-resistant enterococci at a MIC range of 4–8 µg/mL. TCBZ also inhibited key Gram-negative bacteria in the presence of sub-inhibitory concentrations of PMB, returning MIC90 values of 1 µg/mL for Escherichia coli, 8 µg/mL for Klebsiella pneumoniae, 2 µg/mL for Acinetobacter baumannii and 4 µg/mL for Pseudomonasaeruginosa. Interestingly, TCBZ was found to be bacteriostatic against intracellular S. aureus but bactericidal against intracellular S. pseudintermedius. Additionally, TCBZ’s favourable pharmacokinetic (PK) and pharmacodynamic (PD) profile was further explored by in vivo safety and efficacy studies using a bioluminescent mouse model of S. aureus sepsis. We show that repeated four-hourly oral treatment of mice with 50 mg/kg TCBZ after systemic S. aureus challenge resulted in a significant reduction in S. aureus populations in the blood to 18 h post-infection (compared to untreated mice) but did not clear the bacterial infection from the bloodstream, consistent with in vivo bacteriostatic activity. These results indicate that additional pharmaceutical development of TCBZ may enhance its PK/PD, allowing it to be an appropriate candidate for the treatment of serious MDR bacterial pathogens.
Properties and Kinetics of Selective Zinc Leaching with Choline Chloride and Urea
Authors: Zhang Jinxia , Dong Jiajing , Niu Fusheng , Yang Chao
Published: August 2021

A choline chloride-urea (ChCl-urea) deep eutectic solvent (DES) was used to experimentally investigate the secondary recovery of zinc from zinc-bearing dust sludge via a leaching process. The effects of varying the liquid–solid ratio, leaching temperature, stirring speed, and leaching time on the zinc leaching efficiency were determined, and the optimum values of these parameters were found to be 15:1, 90 °C, 400 rpm, and 600 min, respectively, at which a leaching efficiency of 86.87% was achieved. XRF and EDS analyses confirmed that the zinc content in the sludge decreased noticeably after leaching, while those of other elements did not, indicating the selective and efficient leaching of zinc. A study of the leaching kinetics showed that the reaction conforms to the nuclear shrinkage model without solid product layer formation, and the calculated apparent activation energy is 22.16 kJ/mol.
Instability Assessment of Hanging Wall Rocks during Underground Mining of Iron Ores
Authors: Bazaluk Oleg , Petlovanyi Mykhailo , Zubko Serhii , Lozynskyi Vasyl , Sai Kateryna
Published: August 2021

The paper presents the study of the deformation processes development in unstable rocks of the hanging wall during mining a thick steeply dipping ore deposit in the example of the Yuzhno-Belozerskyi deposit. In the studied field, there are problems of stability of hanging wall rocks, represented by low-resistant shale rocks that do not withstand significant outcrops in time. A decrease in stability is manifested in the form of failure of the hanging wall rocks into the stope. Based on a detailed study of the ore deposit geological structure and the performance of the stopes mining, according to the survey data, an area of the deposit has been identified where the ore failure and dilution reach 4%–8% with a maximum value of 12%. This also makes it possible to determine the most important averaged source data for performing physical modeling on equivalent materials. It has been determined that the deformation value of the hanging wall rocks with subsequent failure into the stope and ore mass deformation in the sloping bottom change exponentially with an increase in the depth of the stope location, and the dynamics of increasing rock deformations in the hanging wall is noticeably higher than in the sloping bottom of the stope. This reduces the quality of the mined ore and increases the probability of rock failure area propagation to the hanging wall drifts with their subsequent destruction. The results of physical modeling are characterised by acceptable reliability and are confirmed by a high similarity with the actual data on ore dilution with broken rocks during the stopes development. It has been found that during the formation of a steeply dipping outcrop of stopes with an area of 1200 m2, unstable rocks of the hanging wall are prone to failure of significant volumes. For successful mining and achieving stope element stability, it is recommended to optimise its parameters, the height, width and the value of a steeply dipping outcrop, as well as to preserve the ore pillar in the hanging wall until the ore is broken and drawn from the rest of the stope.
High Performance Liquid Chromatography–Tandem Mass Spectrometry Method for Correlating the Metabolic Changes of Lactate, Pyruvate and L-Glutamine with Induced Tamoxifen Resistant MCF-7 Cell Line Potential Molecular Changes
Authors: Alhusban Ala A. , Albustanji Sokiyna , Hamadneh Lama A. , Shallan Aliaa I.
Published: August 2021

Breast cancer is one of the most prevalent cancers worldwide usually treated with Tamoxifen. Tamoxifen resistance development is the most challenging issue in an initially responsive breast tumor, and mechanisms of resistance are still under investigation. The objective of this study is to develop and validate a selective, sensitive, and simultaneous high performance liquid chromatography–tandem mass spectrometry method to explore the changes in substrates and metabolites in supernatant media of developed Tamoxifen resistance MCF-7 cells. We focus on the determination of lactate, pyruvate, and L-glutamine which enables the tracking of changes in metabolic pathways as a result of the resistance process. Chromatographic separation was achieved within 3.5 min. using a HILIC column (4.6 × 100 mm, 3.5 µm particle size) and mobile phase of 0.05 M acetic acid–ammonium acetate buffer solution pH 3.0: Acetonitrile (40:60 v/v). The linear range was 0.11–2.25, 0.012–0.227, and 0.02–0.20 mM for lactate, pyruvate, and L-glutamine, respectively. Within- and between-run accuracy was in the range 98.94-105.50% with precision (CV, %) of ≤0.86%. The results revealed a significant increase in both lactate and pyruvate production after acquiring the resistant. An increase in L-glutamine levels was also observed and could be attributed to its over production or decline in its consumption. Therefore, further tracking of genes responsible of lactate, pyruvate, and glutamine metabolic pathways should be performed in parallel to provide in-depth explanation of resistance mechanism.
Anti-Inflammatory, Antipyretic, and Analgesic Properties of Potamogeton perfoliatus Extract: In Vitro and In Vivo Study
Authors: Rezq Samar , Mahmoud Mona F. , El-Shazly Assem M. , El Raey Mohamed A. , Sobeh Mansour
Published: August 2021

Natural antioxidants, especially those of plant origins, have shown a plethora of biological activities with substantial economic value, as they can be extracted from agro-wastes and/or under exploited plant species. The perennial hydrophyte, Potamogeton perfoliatus, has been used traditionally to treat several health disorders; however, little is known about its biological and its medicinal effects. Here, we used an integrated in vitro and in vivo framework to examine the potential effect of P. perfoliatus on oxidative stress, nociception, inflammatory models, and brewer’s yeast-induced pyrexia in mice. Our results suggested a consistent in vitro inhibition of three enzymes, namely 5-lipoxygenase, cyclooxygenases 1 and 2 (COX-1 and COX-2), as well as a potent antioxidant effect. These results were confirmed in vivo where the studied extract attenuated carrageenan-induced paw edema, carrageenan-induced leukocyte migration into the peritoneal cavity by 25, 44 and 64% at 200, 400 and 600 mg/kg, p.o., respectively. Moreover, the extract decreased acetic acid-induced vascular permeability by 45% at 600 mg/kg, p.o., and chemical hyperalgesia in mice by 86% by 400 mg/kg, p.o., in acetic acid-induced writhing assay. The extract (400 mg/kg) showed a longer response latency at the 3 h time point (2.5 fold of the control) similar to the nalbuphine, the standard opioid analgesic. Additionally, pronounced antipyretic effects were observed at 600 mg/kg, comparable to paracetamol. Using LC-MS/MS, we identified 15 secondary metabolites that most likely contributed to the obtained biological activities. Altogether, our findings indicate that P. perfoliatus has anti-inflammatory, antioxidant, analgesic and antipyretic effects, thus supporting its traditional use and promoting its valorization as a potential candidate in treating oxidative stress-associated diseases.
Lipoic Acid-Modified Oligoethyleneimine-Mediated miR-34a Delivery to Achieve the Anti-Tumor Efficacy
Authors: Huang Yu , Wang Longxiang , Chen Yingxuan , Han Haobo , Li Quanshun
Published: August 2021

MiR-34a, an important tumor suppressor, has been demonstrated to possess great potential in tumor gene therapy. To achieve the upregulation of miR-34a expression level, an oligoethyleneimine (OEI) derivative was constructed and employed as the carrier through the modification with lipoic acid (LA), namely LA-OEI. In contrast to OEI, the derivative LA-OEI exhibited superior transfection efficiency measured by confocal laser scanning microscopy and flow cytometry, owing to rapid cargo release in the disulfide bond-based reduction sensitive pattern. The anti-proliferation and anti-migration effects were tested after the miR-34a transfection to evaluate the anti-tumor response, using human cervical carcinoma cell line HeLa as a model. The delivery of LA-OEI/miR-34a nanoparticles could achieve obvious anti-proliferative effect caused by the induction of cell apoptosis and cell cycle arrest at G1 phase. In addition, it could inhibit the migration of tumor cells via the downregulation of MMP-9 and Notch-1 level. Overall, the LA-OEI-mediated miR-34a delivery was potential to be used as an effective way in the tumor gene therapy.
Design and Synthesis In Silico Drug-like Prediction and Pharmacological Evaluation of Cyclopolymethylenic Homologous of LASSBio-1514
Authors: Lima Lidia Moreira , da Silva Tiago Fernandes , da Silva Monteiro Carlos Eduardo , Aparecida-Silva Cristiane , Bispo Júnior Walfrido , de Queiroz Aline Cavalcanti , Alexandre-Moreira Magna Suzana , Zapata-Sudo Gisele , Barreiro Eliezer J.
Published: August 2021

Acylhydrazones are still an important framework to the design of new bioactive compounds. As treatment of chronic pain represents a clinical challenge, we decided to modify the structure of LASSBio-1514 (1), previously described as anti-inflammatory and analgesic prototype. Applying the homologation as a strategy for molecular modification, we designed a series of cyclopentyl- (2a–e), cyclobutyl- (3a–e), and cyclopropylacylhydrazones (4a–e) that were synthetized and evaluated in murine models of inflammation and pain. A comparison of their in silico physicochemical and drug-like profile was conducted, as well as their anti-inflammatory and analgesic effect. Compounds 4a (LASSBio-1755) and 4e (LASSBio-1757) displayed excellent in silico drug-like profiles and were identified as new analgesic lead-candidates in acute and chronic model of pain, through oral administration.
Nanonutraceuticals Delivery
Authors: Dini Luciana , Vergallo Cristian
Published: August 2021

Technological innovation, environmental sustainability, health, and wellness are the trajectories explored by current research to identify new strategies for a general improvement of human quality of life [...]
Active Electromagnetically Induced Transparency Effect in Graphene-Dielectric Hybrid Metamaterial and Its High-Performance Sensor Application
Authors: Gao Fan , Yuan Peicheng , Gao Shaojun , Deng Juan , Sun Zhiyu , Jin Guoli , Zeng Guanglu , Yan Bo
Published: August 2021

Electromagnetically induced transparency (EIT) based on dielectric metamaterials has attracted attentions in recent years because of its functional manipulation of electromagnetic waves and high refractive index sensitivity, such as high transmission, sharp phase change, and large group delay, etc. In this paper, an active controlled EIT effect based on a graphene-dielectric hybrid metamaterial is proposed in the near infrared region. By changing the Fermi level of the top-covered graphene, a dynamic EIT effect with a high quality factor (Q-factor) is realized, which exhibits a tunable, slow, light performance with a maximum group index of 2500. Another intriguing characteristic of the EIT effect is its high refractive index sensitivity. In the graphene-covered metamaterial, the refractive index sensitivity is simulated as high as 411 nm/RIU and the figure-of-merit (FOM) is up to 159, which outperforms the metastructure without graphene. Therefore, the proposed graphene-covered dielectric metamaterial presents an active EIT effect in the near infrared region, which highlights its great application potential in deep optical switching, tunable slow light devices, and sensitive refractive index sensors, etc.