bluuebunny/crossref_metadata_2025 huggingface dataset:
Contrasting Signals of the Westerly Index and North Atlantic Oscillation over the Drought Sensitivity of Tree-Ring Chronologies from the Mediterranean Basin
Authors: Casas-Gómez Pablo , Sánchez-Salguero Raúl , Ribera Pedro , Linares Juan C.
Published: June 2020

Extreme drought events are becoming increasingly frequent and extended, particularly in Mediterranean drought-prone regions. In this sense, atmospheric oscillations patterns, such as those represented by the North Atlantic Oscillation (NAO) index and the Westerly Index (WI) have been widely proven as reliable proxies of drought trends. Here, we used the Standardized Precipitation–Evapotranspiration Index (SPEI), as a reliable indicator of drought, to investigate the drought sensitivity of tree-ring width data (TRW) from several long-lived tree species (Abies borisii-regis, Abies cilicica, Abies pinsapo, Cedrus atlantica, Cedrus libanii, Pinus nigra, Pinus heldreichii). NAO and WI relations with TRW were also investigated in order to identify potential non-stationary responses among those drought proxies. Our temporal and spatial analyses support contrasting Mediterranean dipole patterns regarding the drought sensitivity of tree growth for each tree species. The spatial assessment of NAO and WI relationships regarding SPEI and TRW showed on average stronger correlations westward with non-stationary correlations between annual WI index and TRW in all species. The results indicate that the drought variability and the inferred drought-sensitive trees species (e.g., C. atlantica) are related to the NAO and the WI, showing that TRW is a feasible proxy to long-term reconstructions of Westerly Index (WI) variability in the Western Mediterranean region. Spatial variability of drought severity suggests a complex association between NAO and WI, likely modulated by an east–west Mediterranean climate dipole.
β-Caryophyllene Reduces the Inflammatory Phenotype of Periodontal Cells by Targeting CB2 Receptors
Authors: Picciolo Giacomo , Pallio Giovanni , Altavilla Domenica , Vaccaro Mario , Oteri Giacomo , Irrera Natasha , Squadrito Francesco
Published: June 2020

Human gingival fibroblasts (GF) and human oral mucosa epithelial cells (EC) with an inflammatory phenotype represent a valuable experimental paradigm to explore the curative activity of agents to be used in oral mucositis. The role of cannabinoid receptor 2 (CB2) has not yet been investigated in oral mucositis. The aim of this study was to evaluate the therapeutic potential of β-Caryophyllene (BCP), a CB2 agonist, in an in vitro model of oral mucositis. GF and EC were stimulated with LPS (2 µg/mL) alone or in combination with BCP; a group of LPS challenged GF and EC were treated with BCP and AM630, a CB2 antagonist. LPS increased the inflammatory cytokines TNF-α, IL-1β, IL-6 and IL-17A whereas it decreased the anti-inflammatory cytokine IL-13. The upstream signals were identified in an augmented expression of NF-κB and STAT-3 and in reduced mRNA levels of PPARγ and PGC-1α. BCP blunted the LPS-induced inflammatory phenotype and this effect was reverted by the CB2 antagonist AM630. These results suggest that CB2 receptors are an interesting target to develop innovative strategies for oral mucositis and point out that BCP exerts a marked curative effect in a preclinical model of oral mucositis which deserves to be confirmed in a clinical setting.
Pathological Findings in Chronic Inflammatory Demyelinating Polyradiculoneuropathy: A Single-Center Experience
Authors: Luigetti Marco , Romano Angela , Di Paolantonio Andrea , Bisogni Giulia , Rossi Salvatore , Conte Amelia , Madia Francesca , Sabatelli Mario
Published: June 2020

Objective: Segmental demyelination is the pathological hallmark of chronic inflammatory demyelinating polyradiculoneuropathy (CIDP), but other elementary lesions are frequently observed, configuring a series of different pathological pictures. In this article, we review the pathological findings of a large series of sural nerve biopsies from our cohort of CIDP patients. Patients and Methods: Patients with CIDP who underwent nerve biopsy were retrospectively selected from those referred to the Institute of Neurology of the “Università Cattolica del Sacro Cuore” in Rome, Italy, from 1982 to February 2020. Sural nerve biopsy was performed according to standard protocols. Results: Sural nerve biopsy was performed in 43/130 CIDP patients. Demyelinating abnormalities and axonal loss were found in 67.4% and 83.7% of biopsies, respectively. Conversely, onion bulbs and inflammatory infiltrates were rare (18.6% and 4.7%, respectively). In three cases, we observed normal pathological findings. Conclusions: A pathognomonic pathological finding of CIDP cannot be established, but we confirm the utility of nerve biopsy in this setting to confirm the diagnosis (also in atypical phenotypes) and to elucidate pathogenic mechanisms.
Infant Care Practices among Resettled Refugee Mothers from East and Central Africa
Authors: Bader Lauren R. , Ward Jennifer , Fouts Hillary N. , Jaekel Julia
Published: June 2020

Refugees often parent under extreme circumstances. Parenting practices have implications for child outcomes, and parenting in the context of refugee resettlement is likely to be dynamic as parents negotiate a new culture. This study examined African origin mothers’ infant care values and practices related to feeding, carrying, and daily activities following resettlement in the Southeastern region of the U.S. Ten African origin mothers were asked about their infant care practices through semi-structured interviews. Results indicated that mothers valued breastfeeding but often chose to use formula as a supplement or instead of breastfeeding. In addition, participants valued carrying their infants close to the body but used equipment such as strollers. Mothers expressed that perceptions of American culture and rules, social support, interactions with community agencies, and the need to engage in formal employment were factors that influenced their infant care practices.
Customized Therapeutic Surface Coatings for Dental Implants
Authors: Zafar Muhammad Sohail , Fareed Muhammad Amber , Riaz Samiya , Latif Muhammad , Habib Syed Rashid , Khurshid Zohaib
Published: June 2020

Dental implants are frequently used to support fixed or removable dental prostheses to replace missing teeth. The clinical success of titanium dental implants is owed to the exceptional biocompatibility and osseointegration with the bone. Therefore, the enhanced therapeutic effectiveness of dental implants had always been preferred. Several concepts for implant coating and local drug delivery had been developed during the last decades. A drug is generally released by diffusion-controlled, solvent-controlled, and chemical controlled methods. Although a range of surface modifications and coatings (antimicrobial, bioactive, therapeutic drugs) have been explored for dental implants, it is still a long way from designing sophisticated therapeutic implant surfaces to achieve the specific needs of dental patients. The present article reviews various interdisciplinary aspects of surface coatings on dental implants from the perspectives of biomaterials, coatings, drug release, and related therapeutic effects. Additionally, the various types of implant coatings, localized drug release from coatings, and how released agents influence the bone–implant surface interface characteristics are discussed. This paper also highlights several strategies for local drug delivery and their limitations in dental implant coatings as some of these concepts are yet to be applied in clinical settings due to the specific requirements of individual patients.
The Impact of AlxGa1−xN Back Barrier in AlGaN/GaN High Electron Mobility Transistors (HEMTs) on Six-Inch MCZ Si Substrate
Authors: Wang H.Y. , Chiu H.C. , Hsu W.C. , Liu C.M. , Chuang C.Y. , Liu J.Z. , Huang Y.L.
Published: June 2020

In this study, AlGaN/GaN high electron mobility transistors (HEMTs) with AlGaN back barriers (B.B.) were comprehensively investigated based on the different Al mole fractions and thicknesses in the design of the experiments. It was shown that the off-state leakage current can be suppressed following an increase of the Al mole fraction due to the enhancement of the back barrier height. Increasing the AlGaN thickness deteriorated device performance because of the generation of lattice mismatch induced surface defects. The dynamic on-resistance (RON) measurements indicated that the Al mole fraction and thickness of the B.B. both affected the buffer trapping phenomenon. In addition, the thickness of B.B. also influenced the substrate heat dissipation ability which is also a key index for high power RF device applications.
Plant Functional Traits on Tropical Ultramafic Habitats Affected by Fire and Mining: Insights for Reclamation
Authors: Quintela-Sabarís Celestino , Faucon Michel-Pierre , Repin Rimi , Sugau John B. , Nilus Reuben , Echevarria Guillaume , Leguédois Sophie
Published: June 2020

Biodiversity-rich tropical ultramafic areas are currently being impacted by land clearing and particularly by mine activities. The reclamation of ultramafic degraded areas requires a knowledge of pioneer plant species. The objective of this study is to highlight the functional traits of plants that colonize ultramafic areas after disturbance by fire or mining activities. This information will allow trait-assisted selection of candidate species for reclamation. Fifteen plots were established on ultramafic soils in Sabah (Borneo, Malaysia) disturbed by recurrent fires (FIRE plots) or by soil excavation and quarrying (MINE plots). In each plot, soil samples were collected and plant cover as well as species abundances were estimated. Fifteen functional traits related to revegetation, nutrient improvement, or Ni phytomining were measured in sampled plants. Vegetation of both FIRE and MINE plots was dominated by perennials with lateral spreading capacity (mainly by rhizomes). Plant communities displayed a conservative growth strategy, which is an adaptation to low nutrient availability on ultramafic soils. Plant height was higher in FIRE than in MINE plots, whereas the number of stems per plant was higher in MINE plots. Perennial plants with lateral spreading capacity and a conservative growth strategy would be the first choice for the reclamation of ultramafic degraded areas. Additional notes for increasing nutrient cycling, managing competition, and implementing of Ni-phytomining are also provided.
Lactose Intolerance: What Your Breath Can Tell You
Authors: Robles Luelle , Priefer Ronny
Published: June 2020

Lactose intolerance has seen recognized as a clinical syndrome characterized by pain, abdominal distention, flatulence, and diarrhea after the consumption of lactose. Lactose is a common disaccharide found in dairy that requires lactase-phlorizin hydrolase (LPH) to break down into glucose and galactose. A deficiency in this enzyme results in flora bacteria further along in the gastrointestinal tract (GI) tract to metabolize the excess lactose to numerous gases, including H2. Recent studies show that the risk of symptoms after lactose ingestion depends on the dose of lactose, LPH expression, intestinal flora, and sensitivity of the gastrointestinal tract. Currently, there are several diagnostic tests that investigate the biological mechanism of lactose intolerance such as blood, biopsy, genetic, and breath tests. Due to its relatively low cost, availability, and non-invasiveness, the hydrogen breath test (HBT) has become a popular technology to aid in the diagnosis of many gastroenterological diseases, specifically lactose intolerance. Additionally, while administering the HBT there seems to be a lack of uniform criteria amongst the various studies, with many using their own guidelines, which may in turn cause inconsistency with the analysis of the results. With ever improving nanotechnology, novel approaches to expedite and lower the costs of the HBT has become an area of research with significant advancements.
Shortcuts to Adiabaticity for Optical Beam Propagation in Nonlinear Gradient Refractive-Index Media
Authors: Kong Qian , Ying Huimin , Chen Xi
Published: June 2020

In recent years, the concept of “shortcuts to adiabaticity" has been originally proposed to speed up sufficiently slow adiabatic process in various quantum systems without final excitation. Based on the analogy between classical optics and quantum mechanics, we present a study on fast non-adiabatic compression of optical beam propagation in nonlinear gradient refractive-index media by using shortcuts to adiabaticity. We first apply the variational approximation method in nonlinear optics to derive the auxiliary equation for connecting the beam width with the refractive index of the medium. Then, the gradient refractive index is inversely designed through the perfect compression of beam width with the appropriate boundary conditions. Finally, the comparison with conventional adiabatic compression is made, showing the advantage of our shortcuts.
Tsallis Entropy for Cross-Shareholding Network Configurations
Authors: Cerqueti Roy , Rotundo Giulia , Ausloos Marcel
Published: June 2020

In this work, we develop the Tsallis entropy approach for examining the cross-shareholding network of companies traded on the Italian stock market. In such a network, the nodes represent the companies, and the links represent the ownership. Within this context, we introduce the out-degree of the nodes—which represents the diversification—and the in-degree of them—capturing the integration. Diversification and integration allow a clear description of the industrial structure that were formed by the considered companies. The stochastic dependence of diversification and integration is modeled through copulas. We argue that copulas are well suited for modelling the joint distribution. The analysis of the stochastic dependence between integration and diversification by means of the Tsallis entropy gives a crucial information on the reaction of the market structure to the external shocks—on the basis of some relevant cases of dependence between the considered variables. In this respect, the considered entropy framework provides insights on the relationship between in-degree and out-degree dependence structure and market polarisation or fairness. Moreover, the interpretation of the results in the light of the Tsallis entropy parameter gives relevant suggestions for policymakers who aim at shaping the industrial context for having high polarisation or fair joint distribution of diversification and integration. Furthermore, a discussion of possible parametrisations of the in-degree and out-degree marginal distribution—by means of power laws or exponential functions— is also carried out. An empirical experiment on a large dataset of Italian companies validates the theoretical framework.
Determination of High Temperature Corrosion Rates of Steam Boiler Evaporators Using Continuous Measurements of Flue Gas Composition and Neural Networks
Authors: Hardy Tomasz , Kakietek Sławomir , Halawa Krzysztof , Mościcki Krzysztof , Janda Tomasz
Published: June 2020

The use of low-emission combustion techniques in pulverized coal-fired (PC) boilers are usually associated with the formation of a reduced-gas atmosphere near evaporator walls. This increases the risk of high temperature (low oxygen) corrosion processes in coal-fired boilers. The identification of the dynamics and the locations of these processes, and minimizing negative consequences are essential for power plant operation. This paper presents the diagnostic system for determining corrosion risks, based on continuous measurements of flue gas composition in the boundary layer of the combustion chamber, and artificial intelligence techniques. Experience from the implementation of these measurements on the OP-230 hard coal-fired boiler, to identify the corrosion hazard of one of the evaporator walls, has been thoroughly described. The results obtained indicate that the continuous controlling of the concentrations of O2 and CO near the water wall, in combination with the use of neural networks, allows for the forecasting of the corrosion rate of the evaporator. The correlation between flue gas composition and corrosion rate has been demonstrated. At the same time, the analysis of the possibilities of significantly simplifying the measurement system by using neural networks was carried out.
On Mixed Convection Squeezing Flow of Nanofluids
Authors: Khan Sheikh Irfan Ullah , Alzahrani Ebraheem , Khan Umar , Zeb Noreena , Zeb Anwar
Published: June 2020

In this article, the impact of effective Prandtl number model on 3D incompressible flow in a rotating channel is proposed under the influence of mixed convection. The coupled nonlinear system of partial differential equations is decomposed into a highly nonlinear system of ordinary differential equations with aid of suitable similarity transforms. Then, the solution of a nonlinear system of ordinary differential equations is obtained numerically by using Runge–Kutta–Fehlberg (RKF) method. Furthermore, the surface drag force C f and the rate of heat transfer N u are portrayed numerically. The effects of different emerging physical parameters such as Hartmann number (M), Reynold’s number (Re), squeezing parameter ( β ), mixed convection parameter λ , and volume fraction ( φ ) are also incorporated graphically for γ — alumina. Due to the higher viscosity and thermal conductivity ethylene-based nanofluids, it is observed to be an effective common base fluid as compared to water. These observations portrayed the temperature of gamma-alumina ethylene-based nanofluids rising on gamma-alumina water based nanofluids.
Importance of Air Humidity and Contaminations in the Internal and External Transonic Flows
Authors: Wiśniewski P. , Dykas S. , Yamamoto S.
Published: June 2020

The paper presents mathematical modelling of steam condensation in the atmospheric air transonic flows. A single-fluid model was employed. The model was validated against the in-house experimental studies of internal flow in nozzles and against benchmark tests available in literature i.e., RAE2822 transonic airfoil. The impact of air relative humidity on the condensation process under transonic flow conditions was examined. The paper shows that the impact of the condensation process in air transonic flow is non-negligible and has to be taken under consideration in flow simulations. The presence of air contaminants in form of small particles was considered too. Their crucial role in the change of the transonic flow structure was numerically confirmed.
Black–White Risk Differentials in COVID-19 (SARS-COV2) Transmission, Mortality and Case Fatality in the United States: Translational Epidemiologic Perspective and Challenges
Authors: Holmes Laurens , Enwere Michael , Williams Janille , Ogundele Benjamin , Chavan Prachi , Piccoli Tatiana , Chinaka Chinacherem , Comeaux Camillia , Pelaez Lavisha , Okundaye Osatohamwen , Stalnaker Leslie , Kalle Fanta , Deepika Keeti , Philipcien Glen , Poleon Maura , Ogungbade Gbadebo , Elmi Hikma , John Valescia , Dabney Kirk W.
Published: June 2020

Background: Social and health inequities predispose vulnerable populations to adverse morbidity and mortality outcomes of epidemics and pandemics. While racial disparities in cumulative incidence (CmI) and mortality from the influenza pandemics of 1918 and 2009 implicated Blacks with survival disadvantage relative to Whites in the United States, COVID-19 currently indicates comparable disparities. We aimed to: (a) assess COVID-19 CmI by race, (b) determine the Black–White case fatality (CF) and risk differentials, and (c) apply explanatory model for mortality risk differentials. Methods: COVID-19 data on confirmed cases and deaths by selective states health departments were assessed using a cross-sectional ecologic design. Chi-square was used for CF independence, while binomial regression model for the Black–White risk differentials. Results: The COVID-19 mortality CmI indicated Blacks/AA with 34% of the total mortality in the United States, albeit their 13% population size. The COVID-19 CF was higher among Blacks/AA relative to Whites; Maryland, (2.7% vs. 2.5%), Wisconsin (7.4% vs. 4.8%), Illinois (4.8% vs. 4.2%), Chicago (5.9% vs. 3.2%), Detroit (Michigan), 7.2% and St. John the Baptist Parish (Louisiana), 7.9%. Blacks/AA compared to Whites in Michigan were 15% more likely to die, CmI risk ratio (CmIRR) = 1.15, 95% CI, 1.01–1.32. Blacks/AA relative to Whites in Illinois were 13% more likely to die, CmIRR = 1.13, 95% CI, 0.93–1.39, while Blacks/AA compared to Whites in Wisconsin were 51% more likely to die, CmIRR = 1.51, 95% CI, 1.10–2.10. In Chicago, Blacks/AA were more than twice as likely to die, CmIRR = 2.24, 95% CI, 1.36–3.88. Conclusion: Substantial racial/ethnic disparities are observed in COVID-19 CF and mortality with Blacks/AA disproportionately affected across the United States.
Sexual Function in Levothyroxine-Treated Hypothyroid Women and Women without Hypothyroidism: A Case-Control
Authors: Romero-Gómez Benjamín , Guerrero-Alonso Paula , Carmona-Torres Juan Manuel , Laredo-Aguilera José Alberto , Pozuelo-Carrascosa Diana Patricia , Cobo-Cuenca Ana Isabel
Published: June 2020

Background: Levothyroxine is the most common treatment to normalize thyroid hormones levels and to reduce primary hypothyroidism symptoms. Aim: To assess sexual function in women with levothyroxine-treated hypothyroidism and women without hypothyroidism. Methods: A case-control study was performed with 152 women with levothyroxine-treated hypothyroidism and 238 women without hypothyroidism. An online survey was used to collect socio-demographic data and the answers to the Women Sexual Function (WSF) questionnaire. Results: Women with levothyroxine-treated hypothyroidism showed a higher prevalence of sexual dysfunction than women in the control group (31.60% vs. 16.40%), furthermore the presence of hypothyroidism increased the risk of sexual dysfunction (p = 0.002, OR: 2.29 (1.36−3.88)). The most affected domains were ‘desire’ (p < 0.001), ‘arousal’ (p = 0.003) and ‘penetration pain’ (p = 0.020). In hypothyroid women, age increased the risk of sexual dysfunctions (p = 0.009, OR: 1.07 (1.01−1.12)), however when age was adjusted (ANCOVA) the sexual dysfunction remained in women with hypothyroidism in all domains. Conclusions: Hypothyroidism is associated with an increase in the prevalence of sexual dysfunction even if treated with levothyroxine and thyroid-stimulating hormone (TSH) levels are normalized. Relevance to clinical practice: Sexual function in hypothyroid women should be assessed before and after starting the treatment.
I Teach Nursing as a Male Nursing Educator: The East Asian Perspective, Context, and Social Cognitive Career Experiences
Authors: Dos Santos Luis Miguel
Published: June 2020

Social and cultural backgrounds, as well as understanding, play key roles in workforce development and human resource shortages, which are associated with the transition to nursing education and teaching from frontline nursing practices. A qualitative method, with the direction of the general inductive approach, was employed in this study. The researcher collected information from 18 male nursing educators who switched their senior roles (from the frontline and practicing fields to nursing education) at nursing schools in South Korea, Japan, Taiwan, China, and Malaysia. Three interview sessions were used to collect information. Three themes were merged from the information: (i) gender-oriented knowledge, teaching and learning; (ii) respect; and (iii) health promotion. More importantly, participants advocated that their male roles and identities provided uniqueness to patients, students, parents, and the general public concerning Asian customs and practices. Based on the social cognitive career theory, personal goals and achievements of career satisfaction took important roles. Although the general public may not agree with these career decisions, due to gender and social biases, participants continued to contribute their energy and knowledge in the health and social caring professions.
Effect of Exercising with Others on Incident Functional Disability and All-Cause Mortality in Community-Dwelling Older Adults: A Five-Year Follow-Up Survey
Authors: Fujii Yuya , Fujii Keisuke , Jindo Takashi , Kitano Naruki , Seol Jaehoon , Tsunoda Kenji , Okura Tomohiro
Published: June 2020

We clarified the effect of exercising with others on the risks of incident functional disability and all-cause mortality among community-dwelling adults. We used an inventory mail survey with a five-year follow-up for 1520 independently living older adults (mean age: 73.4 ± 6.3 years) in Kasama City, Japan. Subjects responded to a self-reported questionnaire in June 2014. Exercise habits and the presence of exercise partners were assessed. Subjects were classified into three groups: Non-exercise, exercising alone, and exercising with others. Follow-up information and date of incident functional disability and death during the five-year follow-up were collected from the database. To compare the association between exercise habits and functional disability and mortality, Cox regression analysis was conducted. Compared with the non-exercise group, exercising with others had significantly lower hazard ratios (HRs) for functional disability (0.59, 95% confidence interval (CI) 0.40–0.88) and mortality (0.40, 95% CI 0.24–0.66) in the covariate models. Compared with exercising alone, exercising with others decreased the HRs for incident functional disability (0.53, 95% CI: 0.36–0.80) and mortality (0.50, 95% CI 0.29–0.85) rates in the unadjusted model; these associations were not significant in the covariate models. Exercising with others can contribute to functional disability prevention and longevity.
Associations between Intrinsic Heart Rate, P Wave and QT Interval Durations and Pulse Wave Analysis in Patients with Hypertension and High Normal Blood Pressure
Authors: Mozos Ioana , Gug Cristina , Mozos Costin , Stoian Dana , Pricop Marius , Jianu Daniela
Published: June 2020

The present study aimed to explore the relationship between electrocardiographic (ECG) and pulse wave analysis variables in patients with hypertension (HT) and high normal blood pressure (HNBP). A total of 56 consecutive, middle-aged hypertensive and HNBP patients underwent pulse wave analysis and standard 12-lead ECG. Pulse wave velocity (PWV), heart rate, intrinsic heart rate (IHR), P wave and QT interval durations were as follows: 7.26 ± 0.69 m/s, 69 ± 11 beats/minute, 91 ± 3 beats/minute, 105 ± 22 mm and 409 ± 64 mm, respectively. Significant correlations were obtained between PWV and IHR and P wave duration, respectively, between early vascular aging (EVA) and P wave and QT interval durations, respectively. Linear regression analysis revealed significant associations between ECG and pulse wave analysis variables but multiple regression analysis revealed only IHR as an independent predictor of PWV, even after adjusting for blood pressure variables and therapy. Receiver-operating characteristic (ROC) curve analysis revealed P wave duration (area under curve (AUC) = 0.731; 95% CI: 0.569–0.893) as a predictor of pathological PWV, and P wave and QT interval durations were found as sensitive and specific predictors of EVA. ECG provides information about PWV and EVA in patients with HT and HNBP. IHR and P wave durations are independent predictors of PWV, and P wave and QT interval may predict EVA.
The Impact of a Gradual Healthier Assortment among Vocational Schools Participating in a School Canteen Programme: Evidence from Sales and Student Survey Data
Authors: van Kleef Ellen , Kremer Florine , van Trijp Hans C. M.
Published: June 2020

Many adolescents have poor eating habits. As a major part of their caloric intake takes place at school, the present study aims to examine the effect of increasing the availability of healthier foods in school canteens on sales, student attitude and self-reported behaviour. A quasi-experimental study was carried out at two vocational schools in the Netherlands over a 10-month period, where the visible share of healthier products was gradually or abruptly increased from 60% to 80%. Outcome measures were sales data for healthier and less healthy foods and drinks in the canteens, as well as surveys. The proportion of healthier products sold increased from 31.1% during the baseline period to 35.9% in the final period. A gradual increase led to higher relative sales of healthier products (40%) than an abrupt change (34.5%). Survey data showed that students’ moderate satisfaction remained insensitive to the changes over time. Overall, results suggest that increasing the availability of healthier products in school canteens leads to small positive changes in sales of products, particularly in the product groups beverages and sandwiches. A gradual introduction may ensure that students slowly get used to assortment changes.
Upregulation of miR-941 in Circulating CD14+ Monocytes Enhances Osteoclast Activation via WNT16 Inhibition in Patients with Psoriatic Arthritis
Authors: Lin Shang-Hung , Ho Ji-Chen , Li Sung-Chou , Cheng Yu-Wen , Yang Yi-Chien , Chen Jia-Feng , Hsu Chung-Yuan , Nakano Toshiaki , Wang Feng-Sheng , Yang Ming-Yu , Lee Chih-Hung , Hsiao Chang-Chun
Published: June 2020

Psoriatic arthritis (PsA) is a destructive joint disease mediated by osteoclasts. MicroRNAs (miRNAs) regulate several important pathways in osteoclastogenesis. We profiled the expression of miRNAs in CD14+ monocytes from PsA patients and investigated how candidate microRNAs regulate the pathophysiology in osteoclastogenesis. The RNA from circulatory CD14+ monocytes was isolated from PsA patients, psoriasis patients without arthritis (PsO), and healthy controls (HCs). The miRNAs were initially profiled by next-generation sequencing (NGS). The candidate miRNAs revealed by NGS were validated by PCR in 40 PsA patients, 40 PsO patients, and 40 HCs. The osteoclast differentiation and its functional resorption activity were measured with or without RNA interference against the candidate miRNA. The microRNA-941 was selectively upregulated in CD14+ monocytes from PsA patients. Osteoclast development and resorption ability were increased in CD14+ monocytes from PsA patients. Inhibition of miR-941 abrogated the osteoclast development and function while increased the expression of WNT16. After successful treatment, the increased miR-941 expression in CD14+ monocytes from PsA patients was revoked. The expression of miR-941 in CD14+ monocytes is associated with PsA disease activity. MiR-941 enhances osteoclastogenesis in PsA via WNT16 repression. The miR-941 could be a potential biomarker and treatment target for PsA.
Approved and Emerging Disease Modifying Therapies on Neurodegeneration in Multiple Sclerosis
Authors: Bross Madeline , Hackett Melody , Bernitsas Evanthia
Published: June 2020

Multiple sclerosis (MS) is an autoimmune, chronic, progressive disease leading to a combination of inflammation, demyelination, and neurodegeneration throughout the central nervous system (CNS). The outcome of these processes can be visualized in magnetic resonance imaging (MRI) scans as brain atrophy, or brain volume loss (BVL), as well as lesions, “black holes” and spinal cord atrophy. MRI outcomes such as BVL have been used as biomarkers of neurodegeneration and other measures of MS disease progression in clinical research settings. Several FDA-approved medications seek to alleviate disease progression by reducing the impact of such factors as demyelination and neurodegeneration, but there are still many shortcomings that current clinical research aims to mitigate. This review attempts to provide an overview of the FDA-approved medications available for treating multiple sclerosis and their effect on neurodegeneration, measured by BVL.
Apamin Suppresses LPS-Induced Neuroinflammatory Responses by Regulating SK Channels and TLR4-Mediated Signaling Pathways
Authors: Park Jihyun , Jang Kyung Mi , Park Kwan-Kyu
Published: June 2020

Neuroinflammation plays a vital role in neurodegenerative conditions. Microglia are a key component of the neuroinflammatory response. There is a growing interest in developing drugs to target microglia and thereby control neuroinflammatory processes. Apamin (APM) is a specifically selective antagonist of small conductance calcium-activated potassium (SK) channels. However, its effect on neuroinflammation is largely unknown. We examine the effects of APM on lipopolysaccharide (LPS)-stimulated BV2 and rat primary microglial cells. Regarding the molecular mechanism by which APM significantly inhibits proinflammatory cytokine production and microglial cell activation, we found that APM does so by reducing the expression of phosphorylated CaMKII and toll-like receptor (TLR4). In particular, APM potently suppressed the translocation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB)/signal transducer and activator of transcription (STAT)3 and phosphorylated mitogen-activated protein kinases (MAPK)-extracellular signal-regulated kinase (ERK). In addition, the correlation of NF-κB/STAT3 and MAPK-ERK in the neuroinflammatory response was verified through inhibitors. The literature and our findings suggest that APM is a promising candidate for an anti-neuroinflammatory agent and can potentially be used for the prevention and treatment of various neurological disorders.
Mapping of CaM, S100A1 and PIP2-Binding Epitopes in the Intracellular N- and C-Termini of TRPM4
Authors: Bousova Kristyna , Barvik Ivan , Herman Petr , Hofbauerová Kateřina , Monincova Lenka , Majer Pavel , Zouharova Monika , Vetyskova Veronika , Postulkova Klara , Vondrasek Jiri
Published: June 2020

Molecular determinants of the binding of various endogenous modulators to transient receptor potential (TRP) channels are crucial for the understanding of necessary cellular pathways, as well as new paths for rational drug designs. The aim of this study was to characterise interactions between the TRP cation channel subfamily melastatin member 4 (TRPM4) and endogenous intracellular modulators—calcium-binding proteins (calmodulin (CaM) and S100A1) and phosphatidylinositol 4, 5-bisphosphate (PIP2). We have found binding epitopes at the N- and C-termini of TRPM4 shared by CaM, S100A1 and PIP2. The binding affinities of short peptides representing the binding epitopes of N- and C-termini were measured by means of fluorescence anisotropy (FA). The importance of representative basic amino acids and their combinations from both peptides for the binding of endogenous TRPM4 modulators was proved using point alanine-scanning mutagenesis. In silico protein–protein docking of both peptides to CaM and S100A1 and extensive molecular dynamics (MD) simulations enabled the description of key stabilising interactions at the atomic level. Recently solved cryo-Electron Microscopy (EM) structures made it possible to put our findings into the context of the entire TRPM4 channel and to deduce how the binding of these endogenous modulators could allosterically affect the gating of TRPM4. Moreover, both identified binding epitopes seem to be ideally positioned to mediate the involvement of TRPM4 in higher-order hetero-multimeric complexes with important physiological functions.
Bacterial Communities Associated with the Pine Wilt Disease Insect Vector Monochamus alternatus (Coleoptera: Cerambycidae) during the Larvae and Pupae Stages
Authors: Chen Hongjian , Hao Dejun , Wei Zhiqiang , Wang Lujie , Lin Tao
Published: June 2020

Monochamus alternatus is an important insect pest in pine forests of southern China and the dispersing vector of the pine wood nematode, Bursaphelenchus xylophilus, which leads to pine wilt disease (PWD). Microbiome of M. alternatus may contribute to survival of larvae in the host pine trees. In order to investigate the intestinal bacterial structure of M. alternatus during the larvae and pupae stages in host trees, and infer the function of symbiotic bacteria, we used 16S rRNA gene Illumina sequencing to obtain and compare the bacterial community composition in the foregut, midgut, and hindgut of larvae, pupal intestines, larval galleries, and pupal chambers of M. alternatus. The diversity of the bacterial community in larval intestines and pupal intestines were similar, as well as was significantly greater in larval galleries and pupal chambers. Although there were differences in bacterial compositions in different samples, similar components were also found. Proteobacteria and Firmicutes were the two most dominant phyla in all samples, and genera Enterobacter, Raoultella, Serratia, Lactococcus, and Pseudomonas were dominant in both the intestinal samples and plant tissue samples. Enterobacter was the most abundant genus in larval intestines, and Serratia was dominant in pupal intestine. The functions of these dominant and specific bacteria were also predicted through metagenomic analyses. These bacteria may help M. alternatus degrade cellulose and pinene. The specific role of symbiotic bacteria in the infection cycle of PWD also warrants further study in the future.
Precipitation and Transformation of Vaterite Calcium Carbonate in the Presence of Some Organic Solvents
Authors: Konopacka-Łyskawa Donata , Czaplicka Natalia , Łapiński Marcin , Kościelska Barbara , Bray Rafał
Published: June 2020

In this paper, the production of CaCO3 particles via the carbonation route in the reaction of CaCl2 and CO2, using NH3 as a promoter of CO2 absorption, was studied. The solvents used as the reaction media for CaCO3 precipitation were aqueous solutions of methanol, isopropanol and dimethyl sulfoxide (DMSO), in a concentration range of 0–20% (v/v). It was found that the presence of an organic additive influenced the precipitation rate, the content of vaterite in the obtained product, the morphology and the size of the precipitated CaCO3 particles, as well as the rate of its transformation into calcite. The presence of all added organic solvents reduced the vaterite concentration in the produced CaCO3 both at the end of the reaction and after incubation in the reaction medium for 1 h. However, the transformation of vaterite particles into calcite in the tested solutions was slower when the 4 h and 24 h procedures were compared. The interactions of solvents with calcite and vaterite were compared using HPLC tests. DMSO molecules interacted with vaterite particles the most strongly, while the interaction of isopropanol with this polymorph was the weakest. The opposite effect was observed for interactions with calcite particles, and the affinity decreased in the series: isopropanol, methanol, DMSO.
Effect of Al Content in Magnesium Alloy on Microstructure and Mechanical Properties of Laser-Welded Mg/Ti Dissimilar Joints
Authors: Dong Wen , Huang Rongrong , Zhao Hongyun , Gong Xiangtao , Chen Bo , Tan Caiwang
Published: June 2020

Laser penetration welding of magnesium alloys and pure titanium TA2 with unequal thickness was performed. Mg base metal with different Al content (AZ31B, AZ61A, AZ91D) was used to investigate the influence of Al element in microstructure and mechanical properties of Mg/Ti dissimilar joints. The results revealed that the change of Mg base metal did not influence the weld appearance of the joints. Three kinds of joint all presented the best mechanical property when the laser power was 3500 W. With the increase content of Al elements in Mg base metal, a reaction layer was observed which was identified as Ti3Al. The highest enrichment of Al element was obtained and its fraction reached 19.31 at% at the AZ91/TA2 interface. The chemical potential gradient of Al from AZ91 to Ti alloy was higher than that from the other two base metals based on thermodynamic calculation. The maximum fracture load reached 3597 N when AZ61 was employed as the base metal and the fracture position was the Ti base metal. AZ31/TA2 joints failed at the weld seam without necking due to the rapid propagation of cracks at the Mg/Ti interface. The AZ91/TA2 joint failed inside the Mg fusion zone with necking at the middle area of the weld, which resulted from the precipitation of brittle phases such as Mg–Al, Ti–Al phases in the fusion zone of Mg alloys.
Investigation of Electrocatalysts Produced by a Novel Thermal Spray Deposition Method
Authors: Hetaba Walid , Klyushin Alexander Yu. , Falling Lorenz J. , Shin Dongyoon , Mechler Anna K. , Willinger Marc-Georg , Schlögl Robert
Published: June 2020

Common methods to produce supported catalysts include impregnation, precipitation, and thermal spray techniques. Supported electrocatalysts produced by a novel method for thermal spray deposition were investigated with respect to their structural properties, elemental composition, and electrochemical performance. This was done using electron microscopy, X-ray photoelectron spectroscopy, and cyclic voltammetry. Various shapes and sizes of catalyst particles were found. The materials exhibit different activity towards oxidation and reduction of Fe. The results show that this preparation method enables the selection of particle coverage as well as size and shape of the catalyst material. Due to the great variability of support and catalyst materials accessible with this technique, this approach is a useful extension to other preparation methods for electrocatalysts.
Microstructural Analysis of Friction Stir Butt Welded Al-Mg-Sc-Zr Alloy Heavy Gauge Sheets
Authors: Kalashnikova Tatiana , Chumaevskii Andrey , Kalashnikov Kirill , Fortuna Sergei , Kolubaev Evgeny , Tarasov Sergei
Published: June 2020

Friction stir welding (FSW) on a heavy gauge sheet of a hereditary fine-grained Al-Mg-Sc-Zr alloy was carried out to study the specifics of plasticized metal flow and microstructural evolution in different sections and zones of the joint. It was found that the stir zone (SZ) macrostructure may contain either a single or many nugget zones depending on the metal sheet thickness and the seam length. The effect of grain kinking in a thermomechanically affected zone (TMAZ) under pressure from the stir zone metal was discovered. The stir zone metal was fine-grained but had a microhardness lower than that of the base metal, which may be explained by the overaging effect of FSW on the Al3Sc precipitates. The tensile strength of the joint was almost equal to that of the base metal (BM). The grain size distributions were obtained in different sections below the sheet surface and away from the exit hole, which allowed us to suggest the specific adhesion-assisted layer-by layer metal transfer mechanism in FSW.
Importance of Defluviitalea raffinosedens for Hydrolytic Biomass Degradation in Co-Culture with Hungateiclostridium thermocellum
Authors: Rettenmaier Regina , Schneider Martina , Munk Bernhard , Lebuhn Michael , Jünemann Sebastian , Sczyrba Alexander , Maus Irena , Zverlov Vladimir , Liebl Wolfgang
Published: June 2020

Bacterial hydrolysis of polysaccharides is an important step for the production of sustainable energy, for example during the conversion of plant biomass to methane-rich biogas. Previously, Hungateiclostridium thermocellum was identified as cellulolytic key player in thermophilic biogas microbiomes with a great frequency as an accompanying organism. The aim of this study was to physiologically characterize a recently isolated co-culture of H. thermocellum and the saccharolytic bacterium Defluviitalea raffinosedens from a laboratory-scale biogas fermenter. The characterization focused on cellulose breakdown by applying the measurement of cellulose hydrolysis, production of metabolites, and the activity of secreted enzymes. Substrate degradation and the production of volatile metabolites was considerably enhanced when both organisms acted synergistically. The metabolic properties of H. thermocellum have been studied well in the past. To predict the role of D. raffinosedens in this bacterial duet, the genome of D. raffinosedens was sequenced for the first time. Concomitantly, to deduce the prevalence of D. raffinosedens in anaerobic digestion, taxonomic composition and transcriptional activity of different biogas microbiomes were analyzed in detail. Defluviitalea was abundant and metabolically active in reactor operating at highly efficient process conditions, supporting the importance of this organism for the hydrolysis of the raw substrate.
Microanalytical Approaches to Characterizing REE in Appalachian Basin Underclays
Authors: Yang Jon , Montross Scott , Britton Jim , Stuckman Mengling , Lopano Christina , Verba Circe
Published: June 2020

The search for a reliable U.S. domestic source of rare earth elements (REE) is necessary to support the demand of advanced energy applications (e.g., catalysts, electronics, magnets). Sedimentary deposits may be sources for selectively recovering REE and critical metals—specifically the interbedded seat rock, or underclay, that underlies or forms the floor of a coal seam. This material is often a major component of coal waste fines and refuse and thus readily available. This study examines several Appalachian Basin underclays associated with actively mined coal seams as potential feedstocks for the REE. Multimodal microanalytical electron microscopy (SEM, FIB-SEM, EMPA) synchrotron-based µXRF, and image processing techniques are coupled with detailed elemental and mineral data to classify the 2D and 3D petrophysical properties of the materials. The REE contents of Appalachian Basin underclays were measured from 235–399 ppm and predominantly observed as discrete REE-bearing minerals such as monazite and xenotime on the order of 10–100 µm in size. These REE-bearing minerals typically accounted for less than 1% of the scanned areas and volumes under SEM and FIB-SEM analysis, with the exception of regions enriched in crandallite. Synchrotron-based µXRF elemental maps further identified several REE deposition environments in different underclays, including micro-scale (10–100 µm) light REEs co-localizing with Ca and P, micro-scale heavy REEs with Fe, and large-scale light REEs (>200 µm) co-localizing with Sr, Ba, Ca and P.
RETRACTED: Pan et al. Enhanced Adsorption of Zn(II) onto Graphene Oxides Investigated Using Batch and Modeling Techniques. Nanomaterials 2018, 8, 806
Authors: Pan Min , Wu Guangxue , Liu Chang , Lin Xinxin , Huang Xiaoming
Published: June 2020

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Dietary Behaviors That Place Young Adults at Risk for Future Osteoporosis
Authors: Davies Alyse , Rangan Anna , Allman-Farinelli Margaret
Published: June 2020

Dietary behaviors during adolescence and emerging adulthood have important consequences for peak bone mass (PBM) attainment. This study aimed to examine dietary factors that are either beneficial or detrimental to bone health and determine the major sources of calcium in the diets of a sample of young adults. A cross-sectional survey was conducted among 189 Australians aged 18–30 years. Three-day dietary intakes were collected using consecutive 24 h recall interviews. Daily totals for energy and nutrients and serves for food groups were computed. The proportion contribution of calcium (mg) from different food groups as well as calcium (mg) per portion and per 100 g were calculated. Females and males failed to meet the recommendations for dairy (91%, 82%), fruit (89%, 94%) and vegetables (74%, 86%). Eighty percent were above the recommended daily intake range for sodium. For calcium, 53% of females and 48% of males had intakes below the estimated average requirement (EAR). Milk products and dishes made the highest mean calcium contribution per portion (mg) mean standard deviation (SD), 204 mg (212) and accounted for 30% of calcium intake in females and 35% in males. As young adulthood is the final chance for dietary manipulation before PBM is achieved, these dietary risk factors should be addressed.