bluuebunny/crossref_metadata_2025 huggingface dataset:
Identifying Potential Candidate Hub Genes and Functionally Enriched Pathways in the Immune Responses to Quadrivalent Inactivated Influenza Vaccines in the Elderly Through Co-Expression Network Analysis
Authors: Yang Jing , Zhang Jiayou , Fan Renfeng , Zhao Wei , Han Tian , Duan Kai , Li Xinguo , Zeng Peiyu , Deng Jinglong , Zhang Jikai , Yang Xiaoming
Published: December 2020

Insights into the potential candidate hub genes may facilitate the generation of safe and effective immunity against seasonal influenza as well as the development of personalized influenza vaccines for the elderly at high risk of influenza virus infection. This study aimed to identify the potential hub genes related to the immune induction process of the 2018/19 seasonal quadrivalent inactivated influenza vaccines (QIVs) in the elderly ≥60 years by using weighted gene co-expression network analysis (WGCNA). From 63 whole blood samples from16 elderly individuals, a total of 13,345 genes were obtained and divided into eight co-expression modules, with two modules being significantly correlated with vaccine-induced immune responses. After functional enrichment analysis, genes under GO terms of vaccine-associated immunity were used to construct the sub-network for identification and functional validation of hub genes. MCEMP1 and SPARC were confirmed as the hub genes with an obvious effect on QIVs-induced immunity. The MCEMP1 expression was shown to be negatively correlated with the QIVs-associated reactogenicity within 7 days after vaccination, which could be suppressed by the CXCL 8/IL-8 and exacerbated by the Granzyme-B cytotoxic mediator. Meanwhile, the SPARC expression was found to increase the immune responses to the QIVs and contribute to the persistence of protective humoral antibody titers. These two genes can be used to predict QIVs-induced adverse reaction, the intensity of immune responses, and the persistence of humoral antibody against influenza. This work has shed light on further research on the development of personalized QIVs with appropriate immune responses and long-lasting immunity against the forthcoming seasonal influenza.
Preparation and Ultrasonic Imaging Investigation of Perfluoropentane-Filled Polylactic Acid Nanobubbles As a Novel Targeted Ultrasound Contrast Agent
Authors: Xiao Ruolei , Zhao Zhiwei , Chen Jiajuan , He Liu , Wang Huili , Huang Lingping , Luo Binhua
Published: December 2020

In the study reported here, polylactic acid (PLLA) polymer was synthesized using stannous octoate (Sn(Oct)2) and N-(t-butoxycarbonyl) ethanolamine (EABoc) as the catalyst and the initiator, respectively. The selected PLLA polymer with proper molecular weight was used to prepare nanobubbles encapsulating with liquid perfluoropentane. Then, lactoferrin (Lf), which has a good affinity with tumor cells, was conjugated to PLLA nanobubbles. The resulting Lf–PLLA nanobubbles were examined from the perspective of appearance, size, zeta potential, and stability in vitro. The average hydrodynamic diameter of the Lf–PLLA nanobubbles was 315.3 ± 4.2 nm, the polydispersity index (PDI) was 0.153 ± 0.020, and the zeta potential was around −11.3 ± 0.2 mV. Under the transmission electron microscope (TEM), Lf–PLLA nanobubbles were highly dispersed and had a spherical shape with a distinct capsule structure. The Lf–PLLA nanobubbles also showed little cytotoxicity and low hemolysis rate and exhibited good stability in vitro. The enhanced ultrasound imaging ability of Lf–PLLA nanobubbles was detected by an ultrasound imaging system. The results of ultrasound studies in vitro showed that the liquid perfluoropentane underwent phase transition under ultrasonic treatment, which proved the Lf–PLLA nanobubbles could enhance the ability of ultrasonic imaging. The studies of ultrasonic imaging in nude mice bearing subcutaneous tumors showed that the ability of enhanced ultrasonic images was apparent after injection of Lf–PLLA nanobubbles. Acoustic behavior in vitro and in vivo showed that the Lf–PLLA nanobubbles were characterized by strong, stabilized, and the ability of tumor-enhanced ultrasound imaging. Thus, the Lf–PLLA nanobubbles are an effective ultrasound contrast agent for contrast-enhanced imaging.
Neural Substrates of Food Valuation and Its Relationship With BMI and Healthy Eating in Higher BMI Individuals
Authors: Merchant Junaid S. , Cosme Danielle , Giuliani Nicole R. , Dirks Bryce , Berkman Elliot T.
Published: December 2020

Considerable evidence points to a link between body mass index (BMI), eating behavior, and the brain's reward system. However, much of this research focuses on food cue reactivity without examining the subjective valuation process as a potential mechanism driving individual differences in BMI and eating behavior. The current pre-registered study (https://osf.io/n4c95/) examined the relationship between BMI, healthy eating, and subjective valuation of healthy and unhealthy foods in a community sample of individuals with higher BMI who intended to eat more healthily. Particularly, we examined: (1) alterations in neurocognitive measures of subjective valuation related to BMI and healthy eating; (2) differences in the neurocognitive valuation for healthy and unhealthy foods and their relation to BMI and healthy eating; (3) and whether we could conceptually replicate prior findings demonstrating differences in neural reactivity to palatable vs. plain foods. To this end, we scanned 105 participants with BMIs ranging from 23 to 42 using fMRI during a willingness-to-pay task that quantifies trial-by-trial valuation of 30 healthy and 30 unhealthy food items. We measured out of lab eating behavior via the Automated Self-Administered 24 H Dietary Assessment Tool, which allowed us to calculate a Healthy Eating Index (HEI). We found that our sample exhibited robust, positive linear relationships between self-reported value and neural responses in regions previously implicated in studies of subjective value, suggesting an intact valuation system. However, we found no relationship between valuation and BMI nor HEI, with Bayes Factor indicating moderate evidence for a null relationship. Separating the food types revealed that healthy eating, as measured by the HEI, was inversely related to subjective valuation of unhealthy foods. Imaging data further revealed a stronger linkage between valuation of healthy (compared to unhealthy) foods and corresponding response in the ventromedial prefrontal cortex (vmPFC), and that the interaction between healthy and unhealthy food valuation in this region is related to HEI. Finally, our results did not replicate reactivity differences demonstrated in prior work, likely due to differences in the mapping between food healthiness and palatability. Together, our findings point to disruptions in the valuation of unhealthy foods in the vmPFC as a potential mechanism influencing healthy eating.
Impaired Brain Energy Metabolism: Involvement in Depression and Hypothyroidism
Authors: Głombik Katarzyna , Detka Jan , Kurek Anna , Budziszewska Bogusława
Published: December 2020

Although hypothyroidism appears to be an important factor in the pathogenesis of depression, the impact of thyroid hormones on the bioenergetics of the adult brain is still poorly known. Since metabolic changes are reported to be a key player in the manifestation of depressive disorder, we investigated whether there are differences in selected metabolic markers in the frontal cortex and hippocampus of Wistar Kyoto rats (WKY; an animal model of depression) compared to those of control Wistar rats and whether the induction of hypothyroidism by propylthiouracil (PTU) elicits similar effects in these animals or intensifies some parameters in the WKY rats. In our study, we used WKY rats as a model of depression since this strain exhibits lower levels of monoamines in the brain than control rats and exhibits behavioral and hormonal alterations resembling those of depression, including increased reactivity to stress. The findings indicate a decrease in glycolysis intensity in both brain structures in the WKY rats as well as in both strains under hypothyroidism conditions. Furthermore, hypothyroidism disrupted the connection between glycolysis and the Krebs cycle in the frontal cortex and hippocampus in the depression model used in this study. Decreased thyroid hormone action was also shown to attenuate oxidative phosphorylation, and this change was greater in the WKY rats. Our results suggest that both the depression and hypothyroidism models are characterized by similar impairments in brain energy metabolism and mitochondrial function and, additionally, that the co-occurrence of hypothyroidism and depression may exacerbate some of the metabolic changes observed in depression.
The Role of Intratumor Heterogeneity in the Response of Metastatic Non-Small Cell Lung Cancer to Immune Checkpoint Inhibitors
Authors: Nicoś Marcin , Krawczyk Paweł , Crosetto Nicola , Milanowski Janusz
Published: December 2020

Immune checkpoint inhibitors (ICIs) represent one of the most promising therapeutic approaches in metastatic non-small cell lung cancer (M-NSCLC). Unfortunately, approximately 50–75% of patients do not respond to this treatment modality. Intratumor heterogeneity (ITH) at the genetic and phenotypic level is considered as a major cause of anticancer therapy failure, including resistance to ICIs. Recent observations suggest that spatial heterogeneity in the composition and spatial organization of the tumor microenvironment plays a major role in the response of M-NSCLC patients to ICIs. In this mini review, we first present a brief overview of the use of ICIs in M-NSCLC. We then discuss the role of genetic and non-genetic ITH on the efficacy of ICIs in patients with M-NSCLC.
Prediction for Intravenous Immunoglobulin Resistance Combining Genetic Risk Loci Identified From Next Generation Sequencing and Laboratory Data in Kawasaki Disease
Authors: Chen Liqin , Song Sirui , Ning Qianqian , Zhu Danying , Jia Jia , Zhang Han , Zhao Jian , Hao Shiying , Liu Fang , Chu Chen , Huang Meirong , Chen Sun , Xie Lijian , Xiao Tingting , Huang Min
Published: December 2020

Background: Kawasaki disease (KD) is the most common cause of acquired heart disease. A proportion of patients were resistant to intravenous immunoglobulin (IVIG), the primary treatment of KD, and the mechanism of IVIG resistance remains unclear. The accuracy of current models predictive of IVIG resistance is insufficient and doesn't meet the clinical expectations.

Objectives: To develop a scoring model predicting IVIG resistance of patients with KD.

Methods: We recruited 330 KD patients (50 IVIG non-responders, 280 IVIG responders) and 105 healthy children to explore the susceptibility loci of IVIG resistance in Kawasaki disease. A next generation sequencing technology that focused on 4 immune-related pathways and 472 single nucleotide polymorphisms (SNPs) was performed. An R package SNPassoc was used to identify the risk loci, and student's t-test was used to identify risk factors associated with IVIG resistance. A random forest-based scoring model of IVIG resistance was built based on the identified specific SNP loci with the laboratory data.

Results: A total of 544 significant risk loci were found associated with IVIG resistance, including 27 previous published SNPs. Laboratory test variables, including erythrocyte sedimentation rate (ESR), platelet (PLT), and C reactive protein, were found significantly different between IVIG responders and non-responders. A scoring model was built using the top 9 SNPs and clinical features achieving an area under the ROC curve of 0.974.

Conclusions: It is the first study that focused on immune system in KD using high-throughput sequencing technology. Our findings provided a prediction of the IVIG resistance by integrating the genotype and clinical variables. It also suggested a new perspective on the pathogenesis of IVIG resistance.
Synergistic Regulation of Nitrogen and Sulfur on Redox Balance of Maize Leaves and Amino Acids Balance of Grains
Authors: Liu Shuoran , Cui Shuai , Zhang Xue , Wang Yin , Mi Guohua , Gao Qiang
Published: December 2020

As a primary food crop, maize is widely grown around the world. However, the deficiency of essential amino acids, such as lysine, tryptophan, and methionine, results in poor nutritional quality of maize. In addition, the protein concentration of maize declines with the increase in yield, which further reduces the nutritional quality. Here, the photosynthesis of leaves, grain amino acid composition, and stoichiometry of N and S are explored. The results show that N and S maintained the redox balance by increasing the content of glutathione in maize leaves, thereby enhancing the photosynthetic rate and maize yield. Simultaneously, the synergy of N and S increased the grain protein concentration and promoted amino acid balance by increasing the cysteine concentration in maize grains. The maize yield, grain protein concentration, and concentration of essential amino acids, such as lysine, tryptophan, and methionine, could be simultaneously increased in the N:S ratio range of 11.0 to 12.0. Overall, the synergy of N and S simultaneously improved the maize yield and nutritional quality by regulating the redox balance of maize leaves and the amino acids balance of grains, which provides a new theoretical basis and practical method for sustainable production of maize.
Interoceptive Awareness Is Negatively Related to the Exteroceptive Manipulation of Bodily Self-Location
Authors: Bekrater-Bodmann Robin , Azevedo Ruben T. , Ainley Vivien , Tsakiris Manos
Published: December 2020

The perception of being located within one’s body (i.e., bodily self-location) is an essential feature of everyday self-experience. However, by manipulating exteroceptive input, healthy participants can easily be induced to perceive themselves as being spatially dislocated from their physical bodies. It has previously been suggested that interoception, i.e., the processing of inner physiological signals, contributes to the stability of body representations; however, this relationship has not previously been tested for different dimensions of interoception and bodily self-location. In the present study, using an advanced automatized setup, we systematically manipulated participants’ perspective of their own body (first- vs third-person perspective) as well as the synchrony of visuotactile stimulation (synchronous vs asynchronous). The malleability of bodily self-location was assessed using a questionnaire targeting in-body and out-of-body experiences. Participants also performed a heartbeat discrimination task to assess their interoceptive accuracy (behavioral performance), interoceptive sensibility (confidence in their interoceptive abilities), and interoceptive awareness (meta-cognitive representation of interoceptive signals). Bodily self-location was significantly influenced by perspective, with third-person perspective being associated with stronger out-of-body experiences compared to first-person perspective. Furthermore, there was a significant perspective × stimulation interaction, with subsequent analyses showing that participants reported out-of-body experiences particularly under third-person perspective combined with synchronous visuotactile stimulation. Correlation and regression analyses revealed that meta-cognitive interoceptive awareness was specifically and negatively related to the exteroceptively mediated malleability of body experiences. These results indicate that the perception of the self being located within one’s body relies on the interaction of exteroceptive input and higher-order interoceptive abilities. This has implications for theoretical considerations about the bodily self in health as well as for the understanding of disturbed bodily self-processing in clinical contexts.
For Your Eyes Only: A Field Experiment on Nudging Hygienic Behavior
Authors: Mobekk Hilde , Hessen Dag Olav , Fagerstrøm Asle , Jacobsen Hanne
Published: December 2020

These days many gyms and fitness centers are closed to reduce transmission of the SARS-CoV-2 virus in society. The gym is an environment rich in microorganisms, and careful hygiene is a necessity to keep infections at bay. Exercise centers strive for better hygiene compliance among their members. This effort has become essential in light of the current pandemic. Several experimental studies show that others’ physical presence, or the “illusion” of being watched, may alter behavior. This article reports on a natural field experiment testing one specific social nudge intended to increase gym members’ hygienic behavior. The study was conducted before the SARS-COV-2 pandemic. A picture of “observing eyes” was attached to paper dispensers and cleanser spray bottles at two different gyms in Norway. A reversal design, also called an ABA design, with and without the nudge’s presence, was used to investigate the impact on gym members’ hygienic behavior. A follow-up study was conducted in one of the centers to investigate whether the nudge stimuli would function over time. The study included 254 individual choice situations during nine observation sessions conducted over 9 weeks. The results from both centers provide evidence of a strong effect of the nudge. However, the effect decreased during the follow-up study. These findings support previous research indicating that human behavior is influenced by the presence of implicit observation cues – in this case – observing eyes. However, insights into the long-term effect of implicit observation cues are still needed since the salience of the stimuli faded over time.
Cost Assessment of Inpatient Care Episodes of Stroke in Romania
Authors: Lorenzovici László , Székely Andrea , Csanádi Marcell , Gaál Péter
Published: December 2020

Introduction: Stroke is the second leading cause of death worldwide and Romania is no exception. There is a high economic burden associated with the treatment of stroke patients, which puts pressure on the healthcare budget. This study aims to measure the inpatient treatment costs of stroke patients in Romania.

Methods: Our retrospective analysis follows stroke patients in six Romanian hospitals at different progressivity level from different regions. Patients are identified from the official hospital databases, reported for reimbursement purposes. Mean inpatient costs incurred with the treatment of these stroke patient episodes are calculated using the gross costing method. The cost data are derived from the management control system of the study hospitals.

Results: 3,155 patient episodes of stroke were identified in the study hospitals. The average cost per stroke inpatient care episode sums up to EUR 995.57 (95% CI: EUR 963.74—EUR 1 027.39) in 2017, while the overall yearly healthcare burden adds up to EUR 140 million, representing 2.18% of the total national health insurance budget and a cost of EUR 7.15 per capita.

Conclusion: The hospital cost of stroke inpatient care episode in Romania is high and it represents a sizable part of the healthcare budget, but it is among the lowest in Europe, which can mainly be explained by the level of economic development of the country. As both the number of patients and the cost of acute care are expected to increase in the future, the economic burden of stroke is also expected to increase.
Optical See-Through Head-Mounted Displays With Short Focal Distance: Conditions for Mitigating Parallax-Related Registration Error
Authors: Cutolo Fabrizio , Cattari Nadia , Fontana Umberto , Ferrari Vincenzo
Published: December 2020

Optical see-through (OST) augmented reality head-mounted displays are quickly emerging as a key asset in several application fields but their ability to profitably assist high precision activities in the peripersonal space is still sub-optimal due to the calibration procedure required to properly model the user's viewpoint through the see-through display. In this work, we demonstrate the beneficial impact, on the parallax-related AR misregistration, of the use of optical see-through displays whose optical engines collimate the computer-generated image at a depth close to the fixation point of the user in the peripersonal space. To estimate the projection parameters of the OST display for a generic viewpoint position, our strategy relies on a dedicated parameterization of the virtual rendering camera based on a calibration routine that exploits photogrammetry techniques. We model the registration error due to the viewpoint shift and we validate it on an OST display with short focal distance. The results of the tests demonstrate that with our strategy the parallax-related registration error is submillimetric provided that the scene under observation stays within a suitable view volume that falls in a ±10 cm depth range around the focal plane of the display. This finding will pave the way to the development of new multi-focal models of OST HMDs specifically conceived to aid high-precision manual tasks in the peripersonal space.
Bioaugmented Sand Filter Columns Provide Stable Removal of Pesticide Residue From Membrane Retentate
Authors: Ellegaard-Jensen Lea , Schostag Morten Dencker , Nikbakht Fini Mahdi , Badawi Nora , Gobbi Alex , Aamand Jens , Hansen Lars Hestbjerg
Published: December 2020

Drinking water resources, such as groundwater, are threatened by pollution. The pesticide metabolite 2,6-dichlorobenzamide (BAM) is one of the compounds frequently found in groundwater. Studies have attempted to add specific BAM-degrading bacteria to sand filters at drinking water treatment facilities. This biotechnology has shown great potential in removing BAM from contaminated water. However, the degradation potential was formerly lost after ~2–3 weeks due to a decrease of the degrader population over time. The aim of the present study was to overcome the constraints leading to loss of degraders from inoculated filters. Our approach was threefold: (1) Development of a novel inoculation strategy, (2) lowering the flowrate to reduce washout of cells, and (3) increasing the concentration of nutrients hereunder the pollutant in a smaller inlet water stream. The two latter were achieved via modifications of the inlet water by applying membrane treatment which, besides producing an ultra-pure water fraction, produced a residual water stream with nutrients including BAM concentrated in ~ten-fold reduced volume. This was done to alleviate starvation of degrader bacteria in the otherwise oligotrophic sand filters and to enable a decreased flowrate. By this approach, we achieved 100% BAM removal over a period of 40 days in sand filter columns inoculated with the BAM-degraderAminobactersp. MSH1. Molecular targeting of the degrader strain showed that the population of degrader bacteria persisted at high numbers throughout the sand filter columns and over the entire timespan of the experiment. 16S rRNA gene amplicon sequencing confirmed that MSH1 dominated the bacterial communities of the inoculated sand filter columns at experimental termination. The community composition of the indigenous prokaryotes, based on beta diversity, in the sand filter columns was governed by the feed water type i.e., membrane retentate or untreated water.
Perennial Staple Crops: Yields, Distribution, and Nutrition in the Global Food System
Authors: Kreitzman Maayan , Toensmeier Eric , Chan Kai M. A. , Smukler Sean , Ramankutty Navin
Published: December 2020

Staple crops, which have large amounts of carbohydrates, proteins, and/or fats, provide the bulk of calories in people's diets. Perennial plants, which can be productive for many years without the need for replanting, can produce staple foods and environmental benefits, but their agronomic and nutritional properties haven't been considered synthetically in comparison to annual staples. Here we offer a framework to classify perennial staple crops according to their nutritional categories and cultivation status. We assemble literature to report on the yield potential of 51 perennial staple crops, only 15 of which are well-characterized in existing global datasets. We show the extent and distribution of perennial staple crop production in relation to annual crop types, calculate the carbon stocks they hold, and analyze their nutritional content for three macronutrients and nine micronutrients. We found that most perennial staple crops are regional crops (not globally traded) that grow in the subtropics to tropics. At least one perennial staple crop in each of the five nutritional categories has yields over 2.5 t/ha, in some cases considerably higher, competitive with and in many cases exceeding those of nutritionally comparable annual staples. Perennial staple crops only comprise ~4.5% of total cropland. They hold a modest ~11.4 GtC above and below ground, less than one third of the anthropogenic carbon-equivalent emissions for the year 2018, but more than the ~9 GtC held by the same amount of annual cropland. If linear growth in land under perennial staple production continues to 2040, and replaces only annual cropland, an additional ~0.95 GtC could be sequestered. Many perennial crops also had competitive macronutrient density and yield (per unit area) compared to annual staples; moreover, specific perennial staples are abundant in specific micronutrients, indicating that they can be a nutrient-dense part of diets, unlike the most ubiquitous annual staple crops (corn, wheat, rice) that do not appear in the top 85th percentile for any of the nine micronutrients analyzed. Transition of land and diets to perennial staple crops, if judiciously managed, can provide win-win solutions for both food production and ecosystems.
Extracorporeal Shock Wave Therapy Enhances the In Vitro Metabolic Activity and Differentiation of Equine Umbilical Cord Blood Mesenchymal Stromal Cells
Authors: Salcedo-Jiménez Ramés , Koenig Judith B. , Lee Olivia J. , Gibson Thomas W. G. , Madan Pavneesh , Koch Thomas G.
Published: December 2020

Extracorporeal shock wave therapy (ESWT) has been shown to induce different biological effects on a variety of cells, including regulation and stimulation of their function and metabolism. ESWT can promote different biological responses such as proliferation, migration, and regenerations of cells. Recent studies have shown that mesenchymal stromal cells (MSCs) secrete factors that enhance the regeneration of tissues, stimulate proliferation and differentiation of cells, and decrease inflammatory and immune reactions. Clinically, the combination of these two therapies has been used as a treatment for tendon and ligament lesions in horses; however, there is no scientific evidence supporting this combination of therapies in vivo. Therefore, the objectives of the study were to evaluate the effects of ESWT on equine umbilical cord blood mesenchymal stromal cells (CB-MSCs) proliferative, metabolic, migrative, differentiation, and immunomodulatory properties in vitro. Three equine CB-MSC cultures from independent donors were treated using an electrohydraulic shock wave generator attached to a water bath. All experiments were performed as triplicates. Proliferation, viability, migration and immunomodulatory properties of the cells were evaluated. Equine CB-MSCs were induced to evaluate their trilineage differentiation potential. ESWT treated cells had increased metabolic activity, showed positive adipogenic, osteogenic, and chondrogenic differentiation, and showed higher potential for differentiation toward the adipogenic and osteogenic cell fates. ESWT treated cells showed similar immunomodulatory properties to none-ESWT treated cells. Equine CB-MSCs are responsive to ESWT treatment and showed increased metabolic, adipogenic and osteogenic activity, but unaltered immunosuppressive properties. In vivo studies are warranted to determine if synergistic effects occur in the treatment of musculoskeletal injuries if ESWT and equine CB-MSC therapies are combined.
A Low-Cost Miniature Electrohydrostatic Actuator System
Authors: Wiens Travis , Deibert Brendan
Published: December 2020

Hydraulic linear actuators dominate in high power applications but are much less common in low power (<100 W) systems. One reason for this is the cost: electric actuators in this power range generally exhibit lower performance but are also much less expensive than hydraulic systems. However, in recent years, some miniature hydraulic components have been mass produced, driving down prices. This paper presents the application of these low-cost components, together with a novel very low-cost 3D-printed valve to create an electrohydrostatic actuator. Capable of very high power and force density, this system is competitive on cost with lower-performing electric actuators. This paper presents models for the system’s performance, as well as experimental validation data.
N2O and CO2 Emissions from Bare Soil: Effect of Fertilizer Management
Authors: Sosulski Tomasz , Stępień Wojciech , Wąs Adam , Szymańska Magdalena
Published: December 2020

The paper presents the results of a laboratory experiment focused on the assessment of the effect of different methods of application of ammonium nitrate (TD—top dressing and DP—deep placement) on N2O and CO2 emissions from soil without crop cover. Nitrogen application increased soil N2O–N fluxes by 24.3–46.4%, compared to untreated soil (NIL). N2O–N emissions from TD treatment were higher by 12.7%, compared to DP treatment. Soil CO2–C fluxes from DP treatment were significantly higher by 17.2%, compared to those from NIL treatment. Nonetheless, the differences between soil CO2–C fluxes from DP and TD treatments, as well as from TD and NIL treatments, were of no statistical significance. The cumulative greenhouse gas (GHG) emissions (a sum of cumulative soil emissions of CO2–C and N2O–N after conversion to the equivalent of CO2–C) from both N-fertilized soils were similar, and higher by 20% than from untreated soil. The obtained data show that the effect of reduction of N2O–N soil emissions gained by deep placement of nitrogen fertilizer was completely lost through an increase in CO2–C emissions from the soil. This suggests that deep placement of nitrogen fertilizers in sandy soil without crop cover might not lead to a mitigation of soil GHG emissions.
Organic Kale and Cereal Rye Grain Production Following a Sunn Hemp Cover Crop
Authors: Kornecki Ted S. , Balkcom Kipling S.
Published: December 2020

A four-year field experiment was initiated in 2011 at the EV. Smith Research Station, in central Alabama, to determine the effect of sunn hemp (Crotalaria juncea L.) termination methods on organically grown kale (Brassica oleracea, var. acephala L.) for fresh market and cereal rye (Secale cereale L.) for grain. Three different termination methods for the sunn hemp cover crop were chosen: (1) rolling/crimping with an experimental two-stage roller/crimper, (2) rotary mowing, and (3) rotary mowing with incorporation (disking). Kale plots were harvested in the winter and rye plots were harvested in the following spring. Kale plots were fallow from January to June (kept mowed) until planting sunn hemp again across all plots in late spring of the next growing season. Over four growing seasons, average sunn hemp biomass (dry basis) was 10,981 kg ha−1 with plant height of 2.4 m. The average C/N ratio of sunn hemp was 23:1. Sunn hemp biomass amounts differed among growing seasons (from 5589 to 14,720 kg ha−1) due to different weather conditions. Kale yield also varied across growing seasons, with the highest yield of 17,565 kg ha−1 measured in 2012 and the lowest (3915 kg ha−1) in 2014 due to massive weed pressure. Generally, sunn hemp residue management affected kale yield, with greater yields measured for mowed and incorporated residue (15,054 kg ha−1) compared with lower yields for mowed (6758 kg ha−1) and rolled sunn hemp (5559 kg ha−1). Lower yields were related to poor kale seed-to-soil contact (hair pinning) from large amounts of sunn hemp residue on the soil surface. Over four growing seasons, cereal rye grain yield varied among growing seasons, with an average yield of 1358 kg ha−1. Moreover, sunn hemp residue treatments affected grain yield, with greater yields for rolled (1419 kg ha−1) and mowed residue (1467 kg ha−1) compared with a lower yield (1187 kg ha−1) for mowed and incorporated sunn hemp residue.
Impact and Sustainability of Antibiotic Stewardship in Pediatric Emergency Departments: Why Persistence Is the Key to Success
Authors: Barbieri Elisa , De Luca Maia , Minute Marta , D’Amore Carmen , Ciofi Degli Atti Marta Luisa , Martelossi Stefano , Giaquinto Carlo , Da Dalt Liviana , Zaoutis Theoklis , Dona Daniele
Published: December 2020

Antibiotic stewardship programs proved to be effective in improving prescribing appropriateness. This multicenter quasi-experimental study, aimed to assesses the stewardship impact on antibiotics prescribing in different semesters from 2014 to 2019 in three pediatric emergency departments (Center A, B, and C) in Italy. All consecutive patients diagnosed with acute otitis media or pharyngitis were evaluated for inclusion. Two different stewardship were adopted: for Center A and B, clinical pathways were implemented and disseminated, and yearly lectures were held, for Center C, only pathways were implemented. Broad-spectrum prescription rates decreased significantly by 80% for pharyngitis and 29.5 to 55.2% for otitis after the implementation. In Center C, rates gradually increased from the year after the implementation. Amoxicillin dosage adjusted to pharyngitis recommendations in Center C (53.7 vs. 51.6 mg/kg/die; p = 0.011) and otitis recommendations in Center A increasing from 50.0 to 75.0 mg/kg/die (p < 0.001). Days of therapy in children < 24 months with otitis increased from 8.0 to 10.0 in Center A, while in older children decreased in Center A (8.0 vs. 7.0; p < 0.001) and Center B (10.0 vs. 8.0; p < 0.001). Clinical pathways combined with educational lectures is a feasible and sustainable program in reducing broad-spectrum antibiotic prescribing with stable rates over time.
Escherichia coli of Ready-to-Eat (RTE) Meats Origin Showed Resistance to Antibiotics Used by Farmers
Authors: Abass Abdulai , Adzitey Frederick , Huda Nurul
Published: December 2020

Bacterial foodborne infections, including meat-derived infections, are globally associated with diseases and some deaths. Antibiotics are sometimes used to treat bacterial infections. The use of antibiotics by farmers contributes to the development of resistance by foodborne pathogens. This study aimed to investigate the antibiotics used by farmers and the occurrence of antibiotic-resistant Escherichia coli in ready-to-eat (RTE) meat sources. Data was obtained from livestock farmers through the administration of semistructured questionnaires (n = 376) to obtain information on their demographics, knowledge and antibiotic usage. The procedure in the USA Food and Drug Administration (FDA)’s Bacteriological Analytical Manual was used for E. coli detection. Antibiotic resistance test was performed using the disk diffusion method. The findings revealed that most of the farmers were male (74.5%), were aged 30−39 years (28.5%), had tertiary education (30.3%) and had 6−10 years of experience in livestock husbandry. Sheep (65.7%) were the most reared livestock, and antibiotics were mostly used to treat sick animals (36.7%). Tetracycline (27.7%) was the most common antibiotic used by farmers, followed by amoxicillin/clavulanic acid (18.6%) and trimethoprim/sulfamethoxazole (11.7%). Most farmers (56.1%) said they had knowledge of antibiotic usage. The prevalence of E. coli in RTE meats was lowest in pork (6.0%) and highest in chevon (20.0%). E. coli isolates from RTE meats were highly resistant to teicoplanin (96.77%), tetracycline (93.55%), amoxicillin/clavulanic (70.97%), azithromycin (70.97%) and trimethoprim/sulfamethoxazole (58.06%) but was susceptible to chloramphenicol (93.55%), ciprofloxacin (61.29%) and ceftriaxone (58.06%). The multiple antibiotic index ranged from 0.22 to 0.78. Multidrug resistance (93.55%) was high among the E. coli isolates. The resistance pattern AmcAzmTecTeSxt (amoxicillin/clavulanic acid–azithromycin–telcoplanin–tetracycline–trimethoprim/sulfamethoxazole) was the most common. The use of antibiotics by farmers must be well regulated. Sellers of RTE meats also ought to take hygiene practices seriously to keep meat safe and healthy for public consumption.
Vitamin C and Cardiovascular Disease: An Update
Authors: Morelli Marco B. , Gambardella Jessica , Castellanos Vanessa , Trimarco Valentina , Santulli Gaetano
Published: December 2020

The potential beneficial effects of the antioxidant properties of vitamin C have been investigated in a number of pathological conditions. In this review, we assess both clinical and preclinical studies evaluating the role of vitamin C in cardiac and vascular disorders, including coronary heart disease, heart failure, hypertension, and cerebrovascular diseases. Pitfalls and controversies in investigations on vitamin C and cardiovascular disorders are also discussed.
Antioxidants in Cocoa
Authors: Oracz Joanna , Żyżelewicz Dorota
Published: December 2020

Cocoa beans are the seeds of the tropical tree Theobroma cacao L [...]
Case Study: Body Composition Changes Resulting from a Nutritional Intervention on a Professional Vegan Powerlifter
Authors: Hernández-Martínez Carlos , Fernández-Rodríguez Lars , Soriano Marcos Antonio , Martínez-Sanz José Miguel
Published: December 2020

Powerlifting is a weight-class strength sport where achieving low fat mass (FM) and high fat-free mass (FFM) is desirable to improve performance. Recent studies have evaluated the nutritional considerations of different eating patterns, such as vegan diets (VD), in athlete populations. VD are a challenge for athletes who want to attain body composition changes. The aim of this case study is to report on the body composition changes and subjective feelings of a male professional vegan powerlifter following VD for six weeks. The body mass of the powerlifter decreased from 79.3 to 77.4 kg (2.39%). Along with this, FM decreased from 15.0 to 11.4 kg (24%). Conversely, FFM increased from 64.3 to 66.0 kg (2.64%). Moreover, the powerlifter communicated no subjective feelings of low energy availability during training sessions. The VD might compromise adherence in a nutritional intervention which aims to improve body composition due to the nutritional requirements for fat loss. Therefore, more appropriate health assessments, including blood and psychological tests, are required for professional athletes. This short-term VD intervention was satisfactory for improving body composition and no adverse outcomes were reported.
Robocasting of Single and Multi-Functional Calcium Phosphate Scaffolds and Its Hybridization with Conventional Techniques: Design, Fabrication and Characterization
Authors: Mohammadi Mehdi , Pascaud-Mathieu Patricia , Allizond Valeria , Tulliani Jean-Marc , Coppola Bartolomeo , Banche Giuliana , Chaput Christophe , Cuffini Anna Maria , Rossignol Fabrice , Palmero Paola
Published: December 2020

In this work, dense, porous, and, for the first time, functionally-graded bi-layer scaffolds with a cylindrical geometry were produced from a commercially available hydroxyapatite powder using the robocasting technique. The bi-layer scaffolds were made of a dense core part attached to a surrounding porous part. Subsequently, these bi-layer robocast scaffolds were joined with an outer shell of an antibacterial porous polymer layer fabricated by solvent casting/salt leaching techniques, leading to hybrid ceramic-polymer scaffolds. The antibacterial functionality was achieved through the addition of silver ions to the polymer layer. All the robocast samples, including the bi-layer ones, were first characterized through scanning electron microscopy observations, mechanical characterization in compression and preliminary bioactivity tests. Then, the hybrid bi-layer ceramic-polymer scaffolds were characterized through antimicrobial tests. After sintering at 1300 °C for 3 h, the compressive strengths of the structures were found to be equal to 29 ± 4 MPa for dense samples and 7 ± 4 MPa for lattice structures with a porosity of 34.1%. Bioactivity tests performed at 37 °C for 4 weeks showed that the precipitated layer on the robocast samples contained octacalcium phosphate. Finally, it was evidenced that the hybrid structure was effective in releasing antibacterial Ag+ ions to the surrounding medium showing its potential efficiency in limiting Staphylococcus aureus proliferation during surgery.
Assessment of Handler Exposure to Pesticides from Stretcher-Type Power Sprayers in Orchards
Authors: Wang Zhinan , Meng Yuxi , Mei Xiangdong , Ning Jun , Ma Xiaodong , She Dongmei
Published: December 2020

The production and export volume of fruits from China are among the top three in the world. Pesticides are applied to orchards more than 10 times a year to control pests, and stretcher-type power sprayers are widely used to apply chemical pesticides. However, an assessment of pesticide-handler exposure to pesticides in this scenario has not been reported in China. The test pesticide, 30% SYP-9625 concentrate diluted 3000 times, was sprayed on apple orchards in Beijing China. Experiments were conducted to assess dermal and inhalation exposure using standard whole-body dosimetry and air-sampling methodologies. The dermal deposition was the main route of exposure in this study. The dermal unit exposure (UE) of handlers was 350 mg·kg−1 a.i. of SYP-9625. The hands accounted for 59% of the total exposure and were the most exposed body part. Inhalation UE was 0.720 mg·kg−1 a.i. of SYP-9625 and was negligible compared with dermal exposure. We found that use of protective garments while using stretcher-type powers sprayers reduced dermal pesticide exposure. These results can be used as a reference for the handler’s safety in pesticide management and orchard mechanical management.
SCADA Data-Based Support Vector Machine Wind Turbine Power Curve Uncertainty Estimation and Its Comparative Studies
Authors: Pandit Ravi , Kolios Athanasios
Published: December 2020

Power curves, supplied by turbine manufacturers, are extensively used in condition monitoring, energy estimation, and improving operational efficiency. However, there is substantial uncertainty linked to power curve measurements as they usually take place only at hub height. Data-driven model accuracy is significantly affected by uncertainty. Therefore, an accurate estimation of uncertainty gives the confidence to wind farm operators for improving performance/condition monitoring and energy forecasting activities that are based on data-driven methods. The support vector machine (SVM) is a data-driven, machine learning approach, widely used in solving problems related to classification and regression. The uncertainty associated with models is quantified using confidence intervals (CIs), which are themselves estimated. This study proposes two approaches, namely, pointwise CIs and simultaneous CIs, to measure the uncertainty associated with an SVM-based power curve model. A radial basis function is taken as the kernel function to improve the accuracy of the SVM models. The proposed techniques are then verified by extensive 10 min average supervisory control and data acquisition (SCADA) data, obtained from pitch-controlled wind turbines. The results suggest that both proposed techniques are effective in measuring SVM power curve uncertainty, out of which, pointwise CIs are found to be the most accurate because they produce relatively smaller CIs. Thus, pointwise CIs have better ability to reject faulty data if fault detection algorithms were constructed based on SVM power curve and pointwise CIs. The full paper will explain the merits and demerits of the proposed research in detail and lay out a foundation regarding how this can be used for offshore wind turbine conditions and/or performance monitoring activities.
Textile Wastewater Treatment on a Spinning Disc Reactor: Characteristics, Performances, and Empirical Modeling
Authors: Iacob Tudose Eugenia Teodora , Zaharia Carmen
Published: December 2020

Spinning disc (SD) technology has been successfully applied, for the first time, in real textile wastewater treatment with no other additional processing. The SD efficiency was investigated using real textile effluents to study the color and suspended solids removals at different effluent-supplying flowrates (10–30 L/h) and different disc rotational speeds (100–1500 rpm) with good experimental results; thus, it can minimize the polluting loads within a short time period. Furthermore, within this study, process modeling and its classical optimization were applied to SD technology for wastewater treatment. The experiments were organized according to an active central composite rotatable 23 order design, considering as independent variables the wastewater flowrate, rotational speed, and operating time and, as optimization criteria, the suspended solids removal and discoloration degree. Overall, this novel study proved that the SD technology applied in textile effluent treatment is a suitable alternative to a primary mechanical step.
Comprehensive Model for Evaluating the Performance of Mach-Zehnder-Based Silicon Photonic Switch Fabrics in Large Scale
Authors: Kouissi Marouan , Charbonnier Benoit , Algani Catherine
Published: December 2020

Building a large-scale Mach-Zehnder-based silicon photonic switch circuit (LS-MZS) requires an appropriate choice of architecture. In this work, we propose, for the first time to our knowledge, a single metric that can be used to compare different topologies. We propose an accurate analytical model of the signal-to-crosstalk ratio (SCR) that highlights the performance limitations of the main building blocks: Mach-Zehnder interferometers (MZI) and waveguide crossings. It is based on the cumulative crosstalk and total insertion loss of the LS-MZS. Four different architectures: Beneš, dilated Beneš, switch and select, double-layer network were studied for the reason that they are mainly referenced in the literature. We compared them using our developed SCR indicator. With reference to the state-of-the-art technology, the analysis of the four architectures using SCR showed that, on a large scale, a high number of waveguide crossings significantly affects the performance of the switch matrix. Moreover, better performance was reached using the double-layer-network architecture. Then, we presented a 2 × 2 MZI using two electro-optic phase shifters and a waveguide crossing realized in LETI’s silicon photonics technology. Measured performances were quite good: the switch circuit had a crosstalk of −31.3 dB and an insertion loss estimated to be less than 1.31 dB.
Plantar Pressure Evaluation during the Season in Five Basketball Movements
Authors: Amaro Catarina M. , Castro Maria A. , Roseiro Luis , Neto Maria A. , Amaro Ana M.
Published: December 2020

Sports activity is extremely important in the health context, with a clear motivation for its practice. One of the sports that involve more athletes is basketball, where the human body undergoes rapid reactions, emphasizing the contact of the foot with the ground. The main goal of the present study is to evaluate the distribution of plantar pressure in five different basketball movements. Supported by a group of nine volunteer female athletes from a senior basketball team, a data acquisition protocol was defined to identify the changes that occur throughout the sports season. In this study, the maximum values of plantar pressure were evaluated for both feet. The five movements that were defined and studied are all movements that might be performed during the basketball practice period. To guarantee the necessary conditions of data reliability and repeatability, at least seven repetitions were performed for each movement, which occurred at two different moments of the sports season: at the beginning of the competition in November, and at season peak, four months later, in March. Overall, the results obtained did not present statistically significant changes between the two seasons in this study. However, a slight decrease was observed throughout the sporting season for all movements, except for the rebound, where there was a contrary evaluation. Additionally, athletes with a higher level of experience show higher values of plantar pressure than less experienced athletes.