bluuebunny/crossref_metadata_2025 huggingface dataset:
Bending and Torsional Stress Factors in Hypotrochoidal H-Profiled Shafts Standardised According to DIN 3689-1
Authors: Ziaei Masoud
Published: March 2023

Hypotrochoidal profile contours have been produced in industrial applications in recent years using two-spindle processes, and they are considered effective high-quality solutions for form-fit shaft and hub connections. This study mainly concerns analytical approaches to determine the stresses and deformations in hypotrochoidal profile shafts due to pure bending loads. The formulation was developed according to bending principles using the mathematical theory of elasticity and conformal mappings. The loading was further used to investigate the rotating bending behaviour. The stress factors for the classical calculation of maximum bending stresses were also determined for all those profiles presented and compiled in the German standard DIN3689-1 for practical applications. The results were also compared with the corresponding numerical and experimental results, and very good agreement was observed. Additionally, based on previous work, the stress factor was determined for the case of torsional loading to calculate the maximum torsional stresses in the standardised profiles, and the results are listed in a table. This study contributes to the further refinement of the current DIN3689 standard.
A Review of Dielectric Resonator Antenna at Mm-Wave Band
Authors: Alanazi Meshari D.
Published: March 2023

This paper is a comprehensive review of the recent literature studies on the developments and applications of millimeter-wave (mm-wave) dielectric resonator antennas (DRAs). Different designs and techniques for linear and circular polarized DRAs are discussed thoroughly. In addition, array and multiple-input multiple-output (MIMO) DRAs operating in the K, Ka, and V bands are illustrated. These applications are highly advantageous on many levels, resulting in the improved performance of the DRA in terms of obtaining a higher gain, lower losses, a higher efficiency, and a lower profile. This work reviews the fundamental research trends in antennas to meet the demands of fifth-generation (5G) communications and beyond. The reviewed studies are scholarly sources which contain measurement-based results. This paper concludes by highlighting the limitations of the studies and the implications for future research.
On the State-of-the-Art of Solar, Wind, and Other Green Energy Resources and Their Respective Storage Systems
Authors: Wang Sheldon , Tonge Ernuel , Sekanyo Isaac , Portmann Elijah , Azzouz Salim M.
Published: March 2023

In this article, we provide a brief overview of solar photovoltaic and thermal energy, wind turbines with vertical and horizontal axes, and other sustainable energy production systems as well as energy storage systems. In some remote areas away from easy access to electricity and fresh water, a self-contained and self-sustainable off-grid energy production and storage farm is very much needed. In this so-called sustainable energy farm, solar photovoltaic and solar thermal energies along with wind energy can be harvested and stored in a secured battery warehouse with mobile wireless surveillance systems with unpredictable coherent motions. In this battery-based energy storage system, special fire protection measures must also be employed with heat sensors and early detection and alarm systems. The alarm system will be connected to both local fire stations and automated fire extinguishing systems. A subsequent paper will present the details of this wireless fireproof alarm powered by sustainable energy resources. The main purpose of this paper is to document, provide references, and inform about the state-of-the-art achievements in the field of renewable energies, particularly, solar, wind, and other green energy resources.
Electrical and Electronic Waste Management Problems in Africa: Deficits and Solution Approach
Authors: Moyen Massa Gilbert , Archodoulaki Vasiliki-Maria
Published: March 2023

The lack of proper waste management in developing countries results in environmental pollution and human illness. This review presents the available data on the electronic and electrical waste generated and/or transported in Africa. Particular attention is given to waste treatment and the recycling sector, as well as methods for recovering metals from e-waste. The roles and responsibilities of stakeholders and institutions involved in Africa are discussed. Design for Environment guidelines and Sustainable Product Design Concepts are illustrated to find proper strategies for managing e-waste in general, and for Africa in particular. Raising awareness among national and international institutions is necessary to improve e-scraps management in Africa. Measures should be taken to facilitate the transition of e-waste management from the informal to the formal sector, which will create decent jobs and corresponding incomes.
The Contribution of the Hulene-B Waste Dump (Maputo, Mozambique) to the Contamination of Rhizosphere Soils, Edible Plants, Stream Waters, and Groundwaters
Authors: Bernardo Bernardino , Candeias Carla , Rocha Fernando
Published: March 2023

The contamination of ecosystems in areas around waste dumps is a major threat to the health of surrounding populations. The aim of this study is to understand the contribution of the Hulene-B waste dump (Maputo, Mozambique) to the contamination of edible plants, rhizosphere soils, stream waters, and groundwater, and to assess human health risk. Soil and plant samples were analyzed by XRD and XRF for mineralogical and chemical composition characterization, respectively. Mineral phases identified in rhizosphere soil samples were ranked, calcite (CaCO3) > quartz (SiO2) > phyllosilicates (micas and kaolinite) > anhydrite (CaSO4) > K feldspar (KAlSi3O8) > opal (SiO2·nH2O) > gypsum (CaSO4·2H2O), suggesting potential toxic elements low mobility. Soil environmental indices showed pollution by Pb > Cu > Zn > Zr. The chemical composition of edible plants revealed contamination by Ni, Cr, Mn, Fe, Ti, and Zr. Groundwaters and stream waters showed a potential health risk by Hg and, in one irrigation water sample, by Pb content. The health hazard index of rhizosphere soils was higher by ingestion, with children being the ones more exposed. Results suggested a combined health risk by exposure to edible plants, rhizosphere soils, stream waters, and groundwaters.
Genetic Diversity and Population Structure Analysis in the Chinese Endemic Species Michelia crassipes Based on SSR Markers
Authors: Xiao Yuguang , Jiang Xiaolong , Lu Chengcheng , Liu Jun , Diao Shu , Jiang Jingmin
Published: March 2023

Michelia crassipes Y. W. Law (Magnoliaceae) is endemic to China and is the only species with purple flowers in the genus Michelia. It is commonly used as an important parent for flower color improvement and hybrid breeding. M. crassipes is recognized as an endangered plant. An urgent need exists to explore the genetic diversity of M. crassipes to efficiently select hybrid parents and develop efficient conservation strategies. In this study, a total of 128 samples were selected from seven natural populations of M. crassipes to explore their genetic diversity and structure. A total of 14 microsatellite (SSR) markers with high polymorphism and repeatability were developed, and 218 alleles were detected. This study mainly revealed three results: (1) The parameters of expected heterozygosity (He = 0.536) and mean Shannon’s information index (I = 1.121) revealed moderately high levels of genetic diversity for the M. crassipes natural population; (2) The genetic differentiation coefficient (Fst = 0.108) showed that there was a low level of genetic differentiation, and AMOVA indicated that genetic variation existed mainly within populations and that there was frequent gene exchange between populations; and (3) The population genetic structure analysis showed that seven natural populations originated from two ancestral groups, and the Mantel test revealed that genetic and geographical distances between populations were significantly correlated. Our study is the first to explore the genetic diversity and structure of the M. crassipes natural population, which provides an important reference for the collection, conservation and utilization of Michelia crassipes germplasm resources.
Functional Probiotic Foods Development: Trends, Concepts, and Products
Authors: El-Sohaimy Sobhy A. , Hussain Malik A.
Published: March 2023

Functional foods offer positive effects on health beyond basic nutrition [...]
Dairy Fermentation
Authors: Bintsis Thomas
Published: March 2023

Fermented dairy products have been traditionally produced and fermentation has evolved as an effective technique to preserve milk from various milking animals [...]
Biocontrol Using Pythium oligandrum during Malting of Fusarium-Contaminated Barley
Authors: Ng Carlo Antonio , Pernica Marek , Litvanova Katerina , Kolouchova Irena , Branyik Tomas
Published: March 2023

This study investigates the potential of Pythium oligandrum (strains M1 and 00X48) as a biocontrol agent in suppressing the growth of Fusarium culmorum and the production of mycotoxins during the malting of naturally contaminated barley (Hordeum vulgare). The effects of the biocontrol agent on F. culmorum-infected barley malt (BM) were evaluated through real-time PCR and its impact on mycotoxin production was determined by quantitative analysis of deoxynivalenol (DON) and deoxynivalenol-3-glucoside (D3G). The effect of treatment on BM and beer quality were also determined through European Brewery Convention (EBC) standard methods. Optimal treatment with P. oligandrum strains M1 and 00X48 yielded a 59% and 48% reduction in F. culmorum contamination, by 37% and 17% lower DON, and 27% and 32% lower D3G, respectively. BM treated with both P. oligandrum strains exhibited quality enhancement; beer produced from the BM treated with P. oligandrum strain M1 resulted in no quality deterioration and with 26% and 18% less DON and D3G, respectively, transferred to the final product.
The Protective Effects of Korill Product on Carp Fingerlings Reared in High Densities and Challenged with Albendazole Treatment
Authors: Năstac Lacrămioara (Grădinariu) , Dediu Lorena , Crețu Mirela , Rîmniceanu Cristian , Docan Angelica , Grecu Iulia , Dima Floricel Maricel , Stroe Maria Desimira , Vizireanu Camelia
Published: March 2023

The objectives of the present study were to evaluate the potential of the Korill (KO), a product based on krill oil, supplemented in fish feed (5 g/kg feed) to alleviate density-induced stress in carp fingerlings, and its protective capacity in case of anthelmintic drug administration (ABZ—albendazole). Thus, the fish were divided into four groups: LD (low density with normal feed), LD-KO (low density with KO supplementation feed), HD (high density with normal feed), and HD-KO (high density with KO supplementation feed). During the first trial, the fish held under different densities were fed normal feed and KO feed for two months, following a 2 × 2 factorial experimental design. In the second trial, seven fish per tank were subjected, for one week, to albendazole treatment (administered daily by an oral dose of 5 mg/kg body weight). For both trials, blood and plasma samples were used to quantify hematological and biochemical parameters. The results showed that the KO diet alleviated the negative impact of ABZ treatment on liver function and the metabolic profile of carp fingerlings reared in high densities. In addition, KO feeding improved lysozyme activity (LZM) and therefore the immune status of the fish, and reduced oxidative damage in the liver, demonstrated by a decrease of malondialdehyde (MDA) content and an increase of total antioxidant capacity (TAC).
Zebrafish Sexual Behavior in Plain and Enriched Environments: Parameters in the Valproate Model of Autism
Authors: Velázquez-Landa Xiomara , Carrillo Porfirio , Coria-Avila Genaro A. , Herrera-Covarrubias Deissy , García Luis I. , Toledo-Cárdenas María Rebeca , Hernández-Aguilar María Elena , Manzo Jorge
Published: March 2023

Autism is a complex alteration in children’s developing nervous system that manifests in behavioral patterns that do not match those of typical subjects. Moreover, starting at puberty, these children may encounter problems regarding social interaction related to sexual encounters. As studies are scarce, we used the valproate model of autism in the zebrafish to contribute to the knowledge related to sexual behavior in this disorder. Young zebrafish were reproduced, embryos collected, and organized in groups of control or treated with valproic acid, as the autism model. Sexual behavior was recorded in fish from these embryos as they became sexually active. The results show that the zebrafish’s sexual behavior is organized into appetitive, preconsummatory, and consummatory behaviors, as in other vertebrates. In the autism model, the patterns of sexual behavior are present but with significant modifications. The behavior of males was the most affected, while in females, the most affected parameter was oviposition. These results show that, in the autism model of zebrafish, sexual behavior is not suppressed, but it seems that critical changes occur in the neuroendocrine system that reduces reproductive success. Furthermore, the enriched environment was beneficial in maintaining the consummatory behaviors of females and males with autism-like behaviors.
Inhibitory Effect of Isopanduratin A on Adipogenesis: A Study of Possible Mechanisms
Authors: Rungsa Prapenpuksiri , San Htoo Tint , Sritularak Boonchoo , Böttcher Chotima , Prompetchara Eakachai , Chaotham Chatchai , Likhitwitayawuid Kittisak
Published: March 2023

The root of Boesenbergia rotunda, a culinary plant commonly known as fingerroot, has previously been reported to possess anti-obesity activity, with four flavonoids identified as active principles, including pinostrobin, panduratin A, cardamonin, and isopanduratin A. However, the molecular mechanisms underlying the antiadipogenic potential of isopanduratin A remain unknown. In this study, isopanduratin A at non-cytotoxic concentrations (1–10 μM) significantly suppressed lipid accumulation in murine (3T3-L1) and human (PCS-210-010) adipocytes in a dose-dependent manner. Downregulation of adipogenic effectors (FAS, PLIN1, LPL, and adiponectin) and adipogenic transcription factors (SREBP-1c, PPARγ, and C/EBPα) occurred in differentiated 3T3-L1 cells treated with varying concentrations of isopanduratin A. The compound deactivated the upstream regulatory signals of AKT/GSK3β and MAPKs (ERK, JNK, and p38) but stimulated the AMPK-ACC pathway. The inhibitory trend of isopanduratin A was also observed with the proliferation of 3T3-L1 cells. The compound also paused the passage of 3T3-L1 cells by inducing cell cycle arrest at the G0/G1 phase, supported by altered levels of cyclins D1 and D3 and CDK2. Impaired p-ERK/ERK signaling might be responsible for the delay in mitotic clonal expansion. These findings revealed that isopanduratin A is a strong adipogenic suppressor with multi-target mechanisms and contributes significantly to anti-obesogenic activity. These results suggest the potential of fingerroot as a functional food for weight control and obesity prevention.
Marine Capture Fisheries from Western Indian Ocean: An Excellent Source of Proteins and Essential Amino Acids
Authors: Jensen Ida-Johanne , Bodin Nathalie , Govinden Rodney , Elvevoll Edel Oddny
Published: March 2023

The Republic of Seychelles is located in Western-Central Indian Ocean, and marine capture fisheries play a key role in the country’s economic and social life in terms of food security, employment, and cultural identity. The Seychellois are among the highest per capita fish-consuming people in the world, with a high reliance on fish for protein. However, the diet is in transition, moving towards a Western-style diet lower in fish and higher in animal meat and easily available, highly processed foods. The aim of this study was to examine and evaluate the protein content and quality of a wide range of marine species exploited by the Seychelles industrial and artisanal fisheries, as well as to further to assess the contribution of these species to the daily intake recommended by the World Health Organization (WHO). A total of 230 individuals from 33 marine species, including 3 crustaceans, 1 shark, and 29 teleost fish, were collected from the Seychelles waters during 2014–2016. All analyzed species had a high content of high-quality protein, with all indispensable amino acids above the reference value pattern for adults and children. As seafood comprises almost 50% of the consumed animal protein in the Seychelles, it is of particular importance as a source of essential amino acids and associated nutrients, and as such every effort to sustain the consumption of regional seafood should be encouraged.
Development of a DNA Metabarcoding Method for the Identification of Insects in Food
Authors: Hillinger Sophie , Saeckler Julia , Domig Konrad J. , Dobrovolny Stefanie , Hochegger Rupert
Published: March 2023

Insects have the potential to become an efficient and reliable food source for humans in the future and could contribute to solving problems with the current food chain. Analytical methods to verify the authenticity of foods are essential for consumer acceptance. We present a DNA metabarcoding method that enables the identification and differentiation of insects in food. The method, developed on Illumina platforms, is targeting a 200 bp mitochondrial 16S rDNA fragment, which we found to be suitable for distinguishing more than 1000 insect species. We designed a novel universal primer pair for a singleplex PCR assay. Individual DNA extracts from reference samples, DNA extracts from model foods and food products commercially available were investigated. In all of the samples investigated, the insect species were correctly identified. The developed DNA metabarcoding method has a high potential to identify and differentiate insect DNA in the context of food authentication in routine analysis.
Lactobacillus gasseri LG-G12 Restores Gut Microbiota and Intestinal Health in Obesity Mice on Ceftriaxone Therapy
Authors: de Moura e Dias Mariana , da Silva Duarte Vinícius , Mota Lúcio , de Cássia Ávila Alpino Gabriela , dos Reis Louzano Sandra , da Conceição Lisiane , Mantovanie Hilário , Pereira Solange , Oliveira Leandro , de Oliveira Mendes Tiago , Porcellato Davide , do Carmo Gouveia Peluzio Maria
Published: March 2023

Gut microbiota imbalance is associated with the occurrence of metabolic diseases such as obesity. Thus, its modulation is a promising strategy to restore gut microbiota and improve intestinal health in the obese. This paper examines the role of probiotics, antimicrobials, and diet in modulating gut microbiota and improving intestinal health. Accordingly, obesity was induced in C57BL/6J mice, after which they were redistributed and fed with an obesogenic diet (intervention A) or standard AIN-93 diet (intervention B). Concomitantly, all the groups underwent a treatment phase with Lactobacillus gasseri LG-G12, ceftriaxone, or ceftriaxone followed by L. gasseri LG-G12. At the end of the experimental period, the following analysis was conducted: metataxonomic analysis, functional profiling of gut microbiota, intestinal permeability, and caecal concentration of short-chain fatty acids. High-fat diet impaired bacterial diversity/richness, which was counteracted in association with L. gasseri LG-G12 and the AIN-93 diet. Additionally, SCFA-producing bacteria were negatively correlated with high intestinal permeability parameters, which was further confirmed via functional profile prediction of the gut microbiota. A novel perspective on anti-obesity probiotics is presented by these findings based on the improvement of intestinal health irrespective of undergoing antimicrobial therapy or not.
Farmer vs. Industrial Practices: Impact of Variety, Cropping System and Process on the Quality of Durum Wheat Grains and Final Products
Authors: Samson Marie-Françoise , Boury-Esnault Anaïs , Menguy Ewen , Avit Valentin , Canaguier Elodie , Bernazeau Bruno , Lavene Patrice , Chiffoleau Yuna , Akermann Gregori , Moinet Kristel , Desclaux Dominique
Published: March 2023

The consumption of artisanal and organic pasta made on-farm from ancient varieties is increasing in France. Some people, namely, those suffering from digestive disorders following the consumption of industrial pasta, consider these artisanal pasta to be more digestible. Most of them have linked these digestive disorders to the ingestion of gluten. We analyzed in this study the impact of industrial and artisanal practices on the protein quality of durum wheat products. The varieties recommended by the industry (IND) were compared to those used by farmers (FAR): the FAR being on average much richer in protein. However, the solubility of these proteins analyzed by Size Exclusion-High Performance Liquid Chromatography (SE-HPLC) and their in vitro proteolysis by digestive enzymes vary little between the two groups of varieties, while differences between varieties in each group are observable. The location of grain production and the tested cropping systems (zero vs. low input) have a low impact on protein quality. Yet, more contrasting modalities should be studied to validate this point. The type of production process (artisanal vs. industrial) is, among those studied, the factor having the greatest impact on protein compositionPasta produced by the artisanal method contains a higher sodium dodecyl sulfate (SDS)-soluble protein fraction and are more in-vitro proteolyzed. Whether these criteria are indicative of what happens during a consumer’s digestion remains to be determined. It also remains to be assessed which key stages of the process have the greatest influence on protein quality.
Ethanol Extract of Mao Jian Green Tea Attenuates Gastrointestinal Symptoms in a Rat Model of Irritable Bowel Syndrome with Constipation via the 5-hydroxytryptamine Signaling Pathway
Authors: Wu Lei , Gao Liming , Jin Xiang , Chen Zhikang , Qiao Xutong , Cui Xiting , Gao Jianhua , Zhang Liwei
Published: March 2023

In a previous study, we demonstrated that the hydro extract of Mao Jian Green Tea (MJGT) promotes gastrointestinal motility. In this study, the effect of MJGT ethanol extract (MJGT_EE) in treating irritable bowel syndrome with constipation (IBS-C) in a rat model constructed via maternal separation combined with an ice water stimulation was investigated. First, a successful model construction was confirmed through the determination of the fecal water content (FWC) and the smallest colorectal distension (CRD) volume. Then, the overall regulatory effects of MJGT_EE on the gastrointestinal tract were preliminarily evaluated through gastric emptying and small intestinal propulsion tests. Our findings indicated that MJGT_EE significantly increased FWC (p < 0.01) and the smallest CRD volume (p < 0.05) and promoted gastric emptying and small intestinal propulsion (p < 0.01). Furthermore, mechanistically, MJGT_EE reduced intestinal sensitivity by regulating the expression of proteins related to the serotonin (5-hydroxytryptamine; 5-HT) pathway. More specifically, it decreased tryptophan hydroxylase (TPH) expression (p < 0.05) and increased serotonin transporter (SERT) expression (p < 0.05), thereby decreasing 5-HT secretion (p < 0.01), activating the calmodulin (CaM)/myosin light chain kinase (MLCK) pathway, and increasing 5-HT4 receptor (5-HT4R) expression (p < 0.05). Moreover, MJGT_EE enhanced the diversity of gut microbiota, increased the proportion of beneficial bacteria, and regulated the number of 5-HT-related bacteria. Flavonoids may play the role of being active ingredients in MJGT_EE. These findings suggest that MJGT_EE could serve as a potential therapeutic pathway for IBS-C.
Occurrence of Phthalate Esters in Coffee and Risk Assessment
Authors: Velotto Salvatore , Squillante Jonathan , Nolasco Agata , Romano Raffaele , Cirillo Teresa , Esposito Francesco
Published: March 2023

Coffee, one of the most widely consumed beverages in the world, is commercialized as powder and beans in different types of packaging and extracted through several methods. In this regard, the present study focused on evaluating the concentration of two of the most used phthalates in plastic materials (bis(2-ethylhexyl)phthalate (DEHP) and di-butyl phthalate (DBP)) in coffee powder and beverages to assess their migration from different packaging and machines. Furthermore, the levels of exposure to these endocrine disruptors in regular coffee consumers were estimated. Samples of packaged coffee powder/beans (n = 60) from different forms of packaging (multilayer bag, aluminum tin, and paper pod) and coffee beverages (n = 40) that were differently extracted (by professional espresso machine (PEM), Moka pot (MP), and home espresso machine (HEM)) were analyzed by extraction of the lipid fraction, purification, and determination by gas chromatography–mass spectrometry (GC/MS). Risk due to consumption of coffee (1–6 cups) was assessed based on tolerable daily intake (TDI) and incremental lifetime cancer risk (ILCR). No significant differences emerged in DBP and DEHP concentrations among different types of packaging (multilayer, aluminum, and paper), whereas higher levels of DEHP were reported in beverages extracted by PEM (6.65, 2.58–11.32) than by MP (0.78, 0.59–0.91) and HEM (0.83, 0.62–0.98). The presence of higher DEHP levels in coffee beverages than in coffee powder may be due to its leaching through machine components. However, the levels of PAEs did not exceed the specific migration limits (SMLs) set out for food contact materials (FCM), and exposure to PAEs from coffee beverages was low, justifying the small risk due of its consumption. Consequently, coffee can be considered a safe beverage for exposure to some phthalic acid esters (PAEs).
A New Bloody Pulp Selection of Myrobalan (Prunus cerasifera L.): Pomological Traits, Chemical Composition, and Nutraceutical Properties
Authors: Sottile Francesco , Napolitano Assunta , Badalamenti Natale , Bruno Maurizio , Tundis Rosa , Loizzo Monica Rosa , Piacente Sonia
Published: March 2023

A new accession of myrobalan (Prunus cerasifera L.) from Sicily (Italy) was studied for the first time for its chemical and nutraceutical properties. A description of the main morphological and pomological traits was created as a tool for characterization for consumers. For this purpose, three different extracts of fresh myrobalan fruits were subjected to different analyses, including the evaluation of total phenol (TPC), flavonoid (TFC), and anthocyanin (TAC) contents. The extracts exhibited a TPC in the range 34.52–97.63 mg gallic acid equivalent (GAE)/100 g fresh weight (FW), a TFC of 0.23–0.96 mg quercetin equivalent (QE)/100 g FW, and a TAC of 20.24–55.33 cyanidine-3-O-glucoside/100 g FW. LC-HRMS analysis evidenced that the compounds mainly belong to the flavonols, flavan-3-ols, proanthocyanidins, anthocyanins, hydroxycinnamic acid derivatives, and organic acids classes. A multitarget approach was used to assess the antioxidant properties by using FRAP, ABTS, DPPH, and β-carotene bleaching tests. Moreover, the myrobalan fruit extracts were tested as inhibitors of the key enzymes related to obesity and metabolic syndrome (α-glucosidase, α-amylase, and lipase). All extracts exhibited an ABTS radical scavenging activity that was higher than the positive control BHT (IC50 value in the range 1.19–2.97 μg/mL). Moreover, all extracts showed iron-reducing activity, with a potency similar to that of BHT (53.01–64.90 vs 3.26 μM Fe(II)/g). The PF extract exhibited a promising lipase inhibitory effect (IC50 value of 29.61 μg/mL).
Proximate Composition, Retained Water, and Bacterial Load for Two Sizes of Hybrid Catfish (Ictalurus furcatus × Ictalurus punctatus) Fillets at Different Process Steps
Authors: Haque Manirul , Silva Juan L.
Published: March 2023

The catfish processors in the US are required to state the maximum percentage of retained water content (RWC) on the product label. The objectives of our study were to quantify the RWC of processed hybrid catfish fillets from proximate composition and the bacterial load at different processing points. Water content was determined using oven-dry (AOAC950.46,1990) and Near-infrared (NIR) spectroscopy. Protein and fat content were determined by NIR spectrometer. Psychrotrophic (PPC) and Total Coliform (TCC) counts were enumerated using 3MPetrifilmTM. The fillets’ overall baseline water, protein, and fat content were 77.8, 16.7 and 5.7%, respectively. The RWC of final fresh and frozen fillets were ~1.1=/- 2.0% (not significant) and ~4.5%, respectively, and was not fillet size or harvest season dependent. Baseline water content (78.0 vs. 76.0%) was higher (p ≤ 0.05), and fat content (6.0% vs. 8.0%) was lower (p ≤ 0.05) for small (50–150 g) compared to large fillets (150–450 g). Higher (p ≤ 0.05) baseline PPC (~4.2 vs. ~3.0) and TCC (~3.4 vs. ~1.7) were observed for the warm season (April–July) fillets compared to the cold season (Feb–April). This study provides information to processors and others on estimating retained water and microbiological quality of the hybrid catfish fillets over the process line.
Time Series Dataset Survey for Forecasting with Deep Learning
Authors: Hahn Yannik , Langer Tristan , Meyes Richard , Meisen Tobias
Published: March 2023

Deep learning models have revolutionized research fields like computer vision and natural language processing by outperforming traditional models in multiple tasks. However, the field of time series analysis, especially time series forecasting, has not seen a similar revolution, despite forecasting being one of the most prominent tasks of predictive data analytics. One crucial problem for time series forecasting is the lack of large, domain-independent benchmark datasets and a competitive research environment, e.g., annual large-scale challenges, that would spur the development of new models, as was the case for CV and NLP. Furthermore, the focus of time series forecasting research is primarily domain-driven, resulting in many highly individual and domain-specific datasets. Consequently, the progress in the entire field is slowed down due to a lack of comparability across models trained on a single benchmark dataset and on a variety of different forecasting challenges. In this paper, we first explore this problem in more detail and derive the need for a comprehensive, domain-unspecific overview of the state-of-the-art of commonly used datasets for prediction tasks. In doing so, we provide an overview of these datasets and improve comparability in time series forecasting by introducing a method to find similar datasets which can be utilized to test a newly developed model. Ultimately, our survey paves the way towards developing a single widely used and accepted benchmark dataset for time series data, built on the various frequently used datasets surveyed in this paper.
Attitude Control of the Quadrotor UAV with Mismatched Disturbances Based on the Fractional-Order Sliding Mode and Backstepping Control Subject to Actuator Faults
Authors: Sun Hui , Li Jinming , Wang Rui , Yang Kaixin
Published: March 2023

Considering mismatched disturbances, aerodynamic interference, chattering, and actuator failure in the attitude control of the quadrotor unmanned aerial vehicle (UAV), this paper establishes a new quadrotor UAV model with mismatched disturbances, based on quaternion, and designs a fault tolerant controller. First, in order to reduce the chattering of the traditional reaching law, a new reaching law based on the sigmoid function is introduced into the design. Second, the sliding mode control and backstepping control methods are adopted, based on the new fractional-order sliding mode surface when the faults occur in quadrotor UAV actuators, and parameters in the sliding mode control are adaptively adjusted. The simulation results show that the fault tolerant control method can control the attitude of UAV quickly and achieve good robustness.
About Analytical Approximate Solutions of the Van der Pol Equation in the Complex Domain
Authors: Orlov Victor , Chichurin Alexander
Published: March 2023

In the article, the existence of solutions for the Van der Pol differential equation is proved, and the approximate structure of such solutions in the analyticity domain is obtained. In the proof, the majorant method was applied not to the right side of the differential equation, as per usual, but to the solution to the nonlinear differential equation under consideration. Results of the numerical study are presented.
On the Existence Results for a Mixed Hybrid Fractional Differential Equations of Sequential Type
Authors: Arab Meraa , Awadalla Muath , Manigandan Murugesan , Abuasbeh Kinda , Mahmudov Nazim I. , Nandha Gopal Thangaraj
Published: March 2023

In this article, we study the existence of a solution to the mixed hybrid fractional differential equations of sequential type with nonlocal integral hybrid boundary conditions. The main results are established with the aid of Darbo’s fixed point theorem and Hausdorff’s measure of noncompactness method. The stability of the proposed fractional differential equation is also investigated using the Ulam–Hyer technique. In addition, an applied example that supports the theoretical results reached through this study is included.
A Comparison Study of Time-Domain Computation Methods for Piecewise Smooth Fractional-Order Circuit Systems
Authors: Chen Xi , Zheng Feng , Wei Yewen
Published: March 2023

The role of fractional calculus in circuit systems has received increased attention in recent years. In order to evaluate the effectiveness of time-domain calculation methods in the analysis of fractional-order piecewise smooth circuit systems, an experimental prototype is developed, and the effects of three typical calculation methods in different test scenarios are compared and studied in this paper. It is proved that Oustaloup’s rational approximation method usually overestimates the peak-to-peak current and brings in the pulse–voltage phenomenon in piecewise smooth test scenarios, while the results of the two iterative recurrence-form numerical methods are in good agreement with the experimental results. The study results are dedicated to provide a reference for efficiently deploying calculation methods in fractional-order piecewise smooth circuit systems. Some quantitative analysis results are concluded in this paper.
Congestion-Aware Bi-Modal Delivery Systems Utilizing Drones
Authors: Beliaev Mark , Mehr Negar , Pedarsani Ramtin
Published: March 2023

With e-commerce demand rising, logistic operators are investing in alternative delivery methods such as drones. Because of their aerial reach, drones can provide much needed utility in the last mile by taking the load off of vehicles delivering parcels to customers on the road. Our goal is to assess the potential drones have in mitigating traffic congestion. To do so, we develop a mathematical model for a bi-modal delivery system composed of parcels carrying trucks and drones, combining it with an optimization problem that can be solved for the socially optimal routing and allocation policy efficiently. Within this formulation, we include multiple stakeholder perspectives by modeling the objective function in terms of both traffic congestion and parcel latency. This allows our model to quantify the impact of drones on reducing traffic congestion, and simultaneously finds the path routing that minimizes the given objective. To account for the effects of stopping trucks on road latency, we simulate roads shared between trucks and cars by utilizing SUMO. We then use quadratic optimization techniques to test our proposed framework on a variety of real-world transportation networks. Our findings highlight the trade-off between reducing traffic congestion and increasing parcel latency—while routing trucks along less time-efficient paths may alleviate traffic congestion, this disproportionately increases the parcel latency. This suggests the need for a balanced approach that considers both factors when solving for the routing policy.
Cholangioscopy-Assisted Laser Lithotripsy for Treatment of Postcholecystectomy Mirizzi Syndrome: Case Series
Authors: Hristov Bozhidar , Doykov Daniel , Andonov Vladimir , Radev Deyan , Kraev Krasimir , Uchikov Petar , Kostov Gancho , Valova Siyana , Tilkiyan Eduard , Doykova Katya
Published: March 2023

Introduction. Mirizzi syndrome (MS) represents a rare clinical entity caused by impaction of one or multiple stones in the infundibulum of the gall bladder or the cystic duct resulting in partial or complete obstruction of the common hepatic or common bile duct (CBD). Though described more than a century ago, MS is still one of the most challenging diseases in the spectrum of biliary pathology. In recent years, endoscopic treatment has become an increasingly popular treatment modality. Patients and methods. Three consecutive patients subjected to cholangioscopy-assisted laser lithotripsy (CA-LL) for postocholecystectomy MS (pMS) were retrospectively evaluated. Case reports. Successful clearance of the cystic duct was achieved in all patients in one or two sessions. One complication in the form of mild cholangitis was observed. Clinical success was 100%. Discussion. According to current research, CA-LL achieves a high rate of ductal clearance and acceptable complication rate in patients with pMS. A 250 µm laser fiber seems to be the optimal choice for CA-LL. Our results suggest that procedure duration is closely associated to the stone size and possibly to the operator experience. In our opinion, upon obtainment of successful ductal clearance and drainage, prophylactic stenting does not improve clinical outcome. Conclusions. Our results demonstrate that CA-LL is a safe and effective treatment for pMS.
Application of Collagen-Based Hydrogel in Skin Wound Healing
Authors: Zhang Yuan , Wang Yong , Li Ying , Yang Yunpeng , Jin Mingyuan , Lin Xiaoying , Zhuang Zeming , Guo Kai , Zhang Tao , Tan Weiqiang
Published: March 2023

The repair of skin injury has always been a concern in the medical field. As a kind of biopolymer material with a special network structure and function, collagen-based hydrogel has been widely used in the field of skin injury repair. In this paper, the current research and application status of primal hydrogels in the field of skin repair in recent years are comprehensively reviewed. Starting from the structure and properties of collagen, the preparation, structural properties, and application of collagen-based hydrogels in skin injury repair are emphatically described. Meanwhile, the influences of collagen types, preparation methods, and crosslinking methods on the structural properties of hydrogels are emphatically discussed. The future and development of collagen-based hydrogels are prospected, which is expected to provide reference for the research and application of collagen-based hydrogels for skin repair in the future.
Geopolymer Composites—In Environmentally Friendly Aspects
Authors: Kóth János , Sinkó Katalin
Published: March 2023

In the present work, a new, low energy consumption sol–gel synthesis route has been developed for geopolymer composites. Instead of the typically published 0.1–1.0 Al/Si molar ratios, the efforts of this study concentrated on the realization of >2.5 Al/Si molar ratios in the composite systems. The higher Al molar ratio significantly improves the mechanical properties. A very important aim was also the recycling of industrial waste materials with attention to environmentally friendly requirements. The very dangerous, toxic red mud as a waste product of aluminum industrial fabrication was selected for reclamation. The structural investigation was carried out by 27Al MAS NMR, XRD, and thermal analysis. The structural examination has unambiguously proven the composite phases in both gel and solid systems. The characterization of composites was performed with mechanical strength and water solubility measurements.
Genome-Wide Analysis of lncRNA and mRNA Expression in the Uterus of Laying Hens during Aging
Authors: Li Guang , Yang Xinyue , Li Junyou , Zhang Bingkun
Published: March 2023

Eggshell plays an essential role in preventing physical damage and microbial invasions. Therefore, the analysis of genetic regulatory mechanisms of eggshell quality deterioration during aging in laying hens is important for the biosecurity and economic performance of poultry egg production worldwide. This study aimed to compare the differences in the expression profiles of long non-coding RNAs (lncRNAs) and mRNAs between old and young laying hens by the method of high-throughput RNA sequencing to identify candidate genes associated with aging in the uterus of laying hens. Overall, we detected 176 and 383 differentially expressed (DE) lncRNAs and mRNAs, respectively. Moreover, functional annotation analysis based on the Gene Ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) databases revealed that DE-lncRNAs and DE-mRNAs were significantly enriched in “phosphate-containing compound metabolic process”, “mitochondrial proton-transporting ATP synthase complex”, “inorganic anion transport”, and other terms related to eggshell calcification and cuticularization. Through integrated analysis, we found that some important genes such as FGF14, COL25A1, GPX8, and GRXCR1 and their corresponding lncRNAs were expressed differentially between two groups, and the results of quantitative real-time polymerase chain reaction (qPCR) among these genes were also in excellent agreement with the sequencing data. In addition, our study found that TCONS_00181492, TCONS_03234147, and TCONS_03123639 in the uterus of laying hens caused deterioration of eggshell quality in the late laying period by up-regulating their corresponding target genes FGF14, COL25A1, and GRXCR1 as well as down-regulating the target gene GPX8 by TCONS_01464392. Our findings will provide a valuable reference for the development of breeding programs aimed at breeding excellent poultry with high eggshell quality or regulating dietary nutrient levels to improve eggshell quality.
Novel Clustering Methods Identified Three Caries Status-Related Clusters Based on Oral Microbiome in Thai Mother–Child Dyads
Authors: Manning Samantha , Xiao Jin , Li Yihong , Saraithong Prakaimuk , Paster Bruce J. , Chen George , Wu Yan , Wu Tong Tong
Published: March 2023

Early childhood caries (ECC) is a disease that globally affects pre-school children. It is important to identify both protective and risk factors associated with this disease. This paper examined a set of saliva samples of Thai mother–child dyads and aimed to analyze how the maternal factors and oral microbiome of the dyads influence the development of ECC. However, heterogeneous latent subpopulations may exist that have different characteristics in terms of caries development. Therefore, we introduce a novel method to cluster the correlated outcomes of dependent observations while selecting influential independent variables to unearth latent groupings within this dataset and reveal their association in each group. This paper describes the discovery of three heterogeneous clusters in the dataset, each with its own unique mother–child outcome trend, as well as identifying several microbial factors that contribute to ECC. Significantly, the three identified clusters represent three typical clinical conditions in which mother–child dyads have typical (cluster 1), high–low (cluster 2), and low–high caries experiences (cluster 3) compared to the overall trend of mother–child caries status. Intriguingly, the variables identified as the driving attributes of each cluster, including specific taxa, have the potential to be used in the future as caries preventive measures.
Social Diversification Driven by Mobile Genetic Elements
Authors: Weltzer Michael L. , Wall Daniel
Published: March 2023

Social diversification in microbes is an evolutionary process where lineages bifurcate into distinct populations that cooperate with themselves but not with other groups. In bacteria, this is frequently driven by horizontal transfer of mobile genetic elements (MGEs). Here, the resulting acquisition of new genes changes the recipient’s social traits and consequently how they interact with kin. These changes include discriminating behaviors mediated by newly acquired effectors. Since the producing cell is protected by cognate immunity factors, these selfish elements benefit from selective discrimination against recent ancestors, thus facilitating their proliferation and benefiting the host. Whether social diversification benefits the population at large is less obvious. The widespread use of next-generation sequencing has recently provided new insights into population dynamics in natural habitats and the roles MGEs play. MGEs belong to accessory genomes, which often constitute the majority of the pangenome of a taxon, and contain most of the kin-discriminating loci that fuel rapid social diversification. We further discuss mechanisms of diversification and its consequences to populations and conclude with a case study involving myxobacteria.
Mitochondrial Genomes Assembled from Non-Invasive eDNA Metagenomic Scat Samples in Critically Endangered Mammals
Authors: Baeza J. Antonio , Barata Ryan , Rajapakse Dilani , Penaloza Jayra , Harrison Preston , Haberski Adam
Published: March 2023

The abundance of many large-bodied vertebrates, both in marine and terrestrial environments, has declined substantially due to global and regional climate stressors that define the Anthropocene. The development of genetic tools that can serve to monitor population’s health non-intrusively and inform strategies for the recovery of these species is crucial. In this study, we formally evaluate whether whole mitochondrial genomes can be assembled from environmental DNA (eDNA) metagenomics scat samples. Mitogenomes of four different large vertebrates, the panda bear (Ailuropoda melanoleuca), the moon bear (Ursus thibetanus), the Java pangolin (Manis javanica), and the the North Atlantic right whale (Eubalaena glacialis) were assembled and circularized using the pipeline GetOrganelle with a coverage ranging from 12x to 480x in 14 out of 18 different eDNA samples. Partial mitochondrial genomes were retrieved from three other eDNA samples. The complete mitochondrial genomes of the studied species were AT-rich and comprised 13 protein coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and a putative D-loop/control region. Synteny observed in all assembled mitogenomes was identical to that reported for specimens of the same and other closely related species. This study demonstrates that it is possible to assemble accurate whole mitochondrial chromosomes from eDNA samples (scats) using forthright bench and bioinformatics workflows. The retrieval of mitochondrial genomes from eDNA samples represents a tool to support bioprospecting, bio-monitoring, and other non-intrusive conservation strategies in species considered ‘vulnerable’, ‘endangered’, and/or ‘critically endangered’ by the IUCN Red List of Threatened Species.