bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification and Characterization of 24-Dehydrocholesterol Reductase (DHCR24) in the Two-Spotted Cricket, Gryllus bimaculatus
Authors: Mack Yin Shan Isa , Dehari Masatoshi , Morooka Nobukatsu , Nagata Shinji
Published: September 2021

Arthropods, including insects, convert sterols into cholesterol due to the inability to synthesise cholesterol de novo. 24-dehydrocholesterol reductase (DHCR24) plays an important role in the conversion. Not only involving the cholesterol biosynthesis in vertebrates, DHCR24 is required for the conversion of desmosterol into cholesterol in phytophagous insects. The current study extensively examined DHCR24 in omnivorous insects, which feed on both plants and animals, using Gryllus bimaculatus as the experimental model. We identified cDNAs encoding two homologues of DHCR24 from G. bimaculatus, which were designated as GbDHCR24-1 and GbDHCR24-2. Both homologues contained the flavin adenine dinucleotide binding domain, which is a feature of DHCR24. Quantitative polymerase chain reaction revealed that among tissues of adult crickets, fat body and anterior midgut expressed high levels of GbDHCR24s. Both fat body and anterior midgut demonstrated DHCR24 activities in which one of the functions is the conversion of desmosterol into cholesterol in vitro. Knockdown of GbDHCR24-1 significantly reduced the conversion activity in the anterior midgut while knockdown of the GbDHCR24-2 did not. Additionally, the accumulation of desmosterol was detected in a feeding experiment with a specific DHCR24 inhibitor, azacosterol. We finally concluded that GbDHCR24-1 is the major enzyme that facilitates the desmosterol-to-cholesterol-conversion in crickets.
Biological Characterization of Human Autologous Pericardium Treated with the Ozaki Procedure for Aortic Valve Reconstruction
Authors: Gardin Chiara , Morciano Giampaolo , Ferroni Letizia , Mikus Elisa , Tripodi Alberto , Pin Maurizio , Tremoli Elena , Albertini Alberto , Zavan Barbara
Published: September 2021

Background: The Ozaki procedure is an innovative surgical technique aiming at reconstructing aortic valves with human autologous pericardium. Even if this procedure is widely used, a comprehensive biological characterization of the glutaraldehyde (GA)-fixed pericardial tissue is still missing. Methods: Morphological analysis was performed to assess the general organization of pericardium subjected to the Ozaki procedure (post-Ozaki) in comparison to native tissue (pre-Ozaki). The effect of GA treatment on cell viability and nuclear morphology was then investigated in whole biopsies and a cytotoxicity assay was executed to assess the biocompatibility of pericardium. Finally, human umbilical vein endothelial cells were seeded on post-Ozaki samples to evaluate the influence of GA in modulating the endothelialization ability in vitro and the production of pro-inflammatory mediators. Results: The Ozaki procedure alters the arrangement of collagen and elastic fibers in the extracellular matrix and results in a significant reduction in cell viability compared to native tissue. GA treatment, however, is not cytotoxic to murine fibroblasts as compared to a commercially available bovine pericardium membrane. In addition, in in vitro experiments of endothelial cell adhesion, no difference in the inflammatory mediators with respect to the commercial patch was found. Conclusions: The Ozaki procedure, despite alteration of ECM organization and cell devitalization, allows for the establishment of a noncytotoxic environment in which endothelial cell repopulation occurs.
+3179G/A Insulin-Like Growth Factor-1 Receptor Polymorphism: A Novel Susceptibility Contributor in Anti-Ro/SSA Positive Patients with Sjögren’s Syndrome: Potential Clinical and Pathogenetic Implications
Authors: Skarlis Charalampos , Marketos Nikolaos , Nezos Adrianos , Papanikolaou Asimina , Voulgarelis Michael , Koutsilieris Michael , Moutsopoulos Haralampos M. , Mavragani Clio P.
Published: September 2021

Background: Alterations of the insulin-like growth factor (IGF) pathway along with genetic variations of the IGF1 receptor (IGF1R) gene have been linked to the development of systemic autoimmunity, possibly through apoptosis induction. This study aims to investigate whether genetic variations of the IGF1R contribute to Sjögren’s syndrome (SS) pathogenesis and explores potential functional implications. Methods: DNA extracted from whole peripheral blood derived from 277 primary SS patients, complicated or not by lymphoma, and 337 Healthy controls (HC) was genotyped for the rs2229765 IGF1R polymorphism using the RFLP-PCR assay. Gene expression of IGF1R and IGF1 isoforms, caspases 1, 4, and 5, and inflammasome components NLRP3, ASC, IL1β, IL18, IL33, IGFBP3, and IGFBP6 were quantitated by RT-PCR in total RNA extracted from minor salivary gland biopsies (MSGs) of 50 SS patients and 13 sicca controls (SCs). In addition, IGF1R immunohistochemical (IHC) expression was assessed in formalin-fixed, paraffin-embedded MSG tissue sections derived from 10 SS patients and 5 SCs. Results: The prevalence of the A/A genotype of the rs2229765 IGF1R polymorphism was significantly higher in the anti-Ro/SSA positive SS population compared to healthy controls (24.8% vs. 10.7%, p = 0.001). Moreover, IGF1Rs at both mRNA and protein levels were reduced in SS-derived MSGs compared to SCs and were negatively associated with caspase 1 transcripts. The latter were positively correlated with NLRP3, ASC, and IL1β at the salivary gland tissue level. IGF1R expression in peripheral blood was negatively correlated with ESR and IgG serum levels and positively correlated with urine-specific gravity values. Conclusions: The rs2229765 IGF1R variant confers increased susceptibility for seropositive primary SS. Dampened IGF1R mRNA and protein expression in salivary gland tissues could be related to increased apoptosis and subsequently to the activation of inflammasome pathways.
Sex-Dependent Cortical Volume Changes in Patients with Degenerative Cervical Myelopathy
Authors: Oughourlian Talia C. , Wang Chencai , Salamon Noriko , Holly Langston T. , Ellingson Benjamin M.
Published: September 2021

Degenerative cervical myelopathy (DCM) is a progressive condition characterized by degeneration of osseocartilaginous structures within the cervical spine resulting in compression of the spinal cord and presentation of clinical symptoms. Compared to healthy controls (HCs), studies have shown DCM patients experience structural and functional reorganization in the brain; however, sex-dependent cortical differences in DCM patients remains largely unexplored. In the present study, we investigate the role of sex differences on the structure of the cerebral cortex in DCM and determine how structural differences may relate to clinical measures of neurological function. T1-weighted structural MRI scans were acquired in 85 symptomatic and asymptomatic patients with DCM and 90 age-matched HCs. Modified Japanese Orthopedic Association (mJOA) scores were obtained for patients. A general linear model was used to determine vertex-level significant differences in gray matter volume (GMV) between the following groups (1) male HCs and female HCs, (2) male patients and female patients, (3) male patients and male HCs, and (4) female patients and female HCs. Within patients, males exhibited larger GMV in motor, language, and vision related brain regions compared to female DCM patients. Males demonstrated a significant positive correlation between GMV and mJOA score, in which patients with worsening neurological symptoms exhibited decreasing GMV primarily across somatosensory and motor related cortical regions. Females exhibited a similar association, albeit across a broader range of cortical areas including those involved in pain processing. In sensorimotor regions, female patients consistently showed smaller GMV compared with male patients, independent of mJOA score. Results from the current study suggest strong sex-related differences in cortical volume in patients with DCM, which may reflect hormonal influence or differing compensation mechanisms.
The COVID-19 Pandemic Led to a Small Increase in Changed Mentality Regarding Infection Risk without Any Change in Willingness to Be Vaccinated in Chronic Diseases Patients
Authors: Breuker Cyril , Guedj Anne Marie , Allan Mathilde , Coinus Loick , Molinari Nicolas , Chapet Nicolas , Roubille François , Le Quintrec Moglie , Duhalde Véronique , Jouglen Julien , Cestac Philippe , Kinowski Jean Marie , Faure Stéphanie , Faucanie Marie , Lohan Laura , Villiet Maxime , Altwegg Romain , Sultan Ariane
Published: September 2021

The objective of this study was to assess the impact of the COVID-19 pandemic on patients’ perceptions regarding infection risk and vaccination in subjects suffering from chronic diseases. A prospective observational multicentric study conducted from December 2020 to April 2021 in three French University Hospitals. Patients with chronic diseases were proposed to complete a questionnaire regarding the impact of the COVID-19 pandemic on infectious risk knowledge and vaccination. A total of 1151 patients were included and analyzed (62% of which were people with diabetes). The COVID-19 pandemic increased awareness of infectious risks by 19.3%, significantly more in people with diabetes (23.2%, from 54.4% to 67.0%, p < 0.01) when compared to the other high-risk patients (12.5%, from 50.5% to 56.8%, p = 0.06). Respectively, 30.6% and 16.5% of patients not up-to-date for pneumococcal and flu vaccines reported wanting to update their vaccination due to the COVID-19 pandemic. By contrast, the proportion of patients against vaccines increased during the COVID-19 pandemic (6.0% vs. 9.5%, p < 0.01). The COVID-19 pandemic has led to a small increase in awareness regarding the risks of infection in patients with chronic diseases, including people with diabetes, but without any change in willingness to be vaccinated. This underlines the urgent need to sensibilize people with diabetes to infection risk and the importance of vaccination.
Our Daily Communicative Contexts: More Evidence for the Food Environment Puzzle
Authors: Abril Eulàlia P. , O’Connell Carrie
Published: September 2021

Much of the research on food environments suggests that problematic access to healthy food restricts its consumption, contributing to adverse outcomes such as obesity, stroke, and heart disease. In addition to these direct relationships, we propose indirect relationships involving the Communication Mediation Model (CMM). Using nationally representative survey data from 1435 adults in the United States, we explore the multipronged role that perceived food environments play in shaping communication, attitudes, and reflective food consumption, considering communicative contexts. Results indicate a negative correlation between the perception of a difficult food environment and reflective consumption, supporting prior research. Most importantly, the food environment is also negatively correlated with news media use, discussion, and attitudes about healthy eating within the CMM. Our findings suggest that indirect communication effects of the food environment on reflective consumption compound the detrimental role of its direct effects.
Pharmacogenomics Variability of Lipid-Lowering Therapies in Familial Hypercholesterolemia
Authors: Hindi Nagham N. , Alenbawi Jamil , Nemer Georges
Published: September 2021

The exponential expansion of genomic data coupled with the lack of appropriate clinical categorization of the variants is posing a major challenge to conventional medications for many common and rare diseases. To narrow this gap and achieve the goals of personalized medicine, a collaborative effort should be made to characterize the genomic variants functionally and clinically with a massive global genomic sequencing of “healthy” subjects from several ethnicities. Familial-based clustered diseases with homogenous genetic backgrounds are amongst the most beneficial tools to help address this challenge. This review will discuss the diagnosis, management, and clinical monitoring of familial hypercholesterolemia patients from a wide angle to cover both the genetic mutations underlying the phenotype, and the pharmacogenomic traits unveiled by the conventional and novel therapeutic approaches. Achieving a drug-related interactive genomic map will potentially benefit populations at risk across the globe who suffer from dyslipidemia.
Ratings of the Effectiveness of Nutraceuticals for Autism Spectrum Disorders: Results of a National Survey
Authors: Adams James B. , Bhargava Anisha , Coleman Devon M. , Frye Richard E. , Rossignol Daniel A.
Published: September 2021

Autism spectrum disorder (ASD) often involves a wide range of co-occurring medical conditions (“comorbidities”) and biochemical abnormalities such as oxidative stress and mitochondrial dysfunction. Nutritional supplements (“Nutraceuticals”) are often used to treat both core ASD symptoms and comorbidities, but some have not yet been formally evaluated in ASD. The potential biological mechanisms of nutraceuticals include correction of micronutrient deficiencies due to a poor diet and support for metabolic processes such as redox regulation, mitochondrial dysfunction and melatonin production. This paper reports on the results of the National Survey on Treatment Effectiveness for Autism, focusing on nutraceuticals. The Survey involved 1286 participants from across the United States. Participants rated the overall perceived benefits and adverse effects of each nutraceutical, and also indicated the specific symptoms changed and adverse effects. From these ratings the top-rated nutraceuticals for each of 24 symptoms are listed. Compared to psychiatric and seizure medications rated through the same Survey, on average nutraceuticals had significantly higher ratings of Overall Benefit (1.59 vs. 1.39, p = 0.01) and significantly lower ratings of Overall Adverse Effects (0.1 vs. 0.9, p < 0.001). Folinic acid and vitamin B12 were two of the top-rated treatments. This study suggests that nutraceuticals may have clinical benefits and favorable adverse effect profiles.
High SPIN4 Expression Is Linked to Advanced Nodal Status and Inferior Prognosis in Nasopharyngeal Carcinoma Patients
Authors: Chang Shih-Lun , Chan Ti-Chun , Chen Tzu-Ju , Yang Ching-Chieh , Tsai Hsin-Hwa , Yeh Cheng-Fa , Lee Sung-Wei , Lai Hong-Yue
Published: September 2021

Nasopharyngeal carcinoma (NPC), characterized by the infiltration of lymphocytes, is a malignancy derived from the epithelium of the nasopharynx. Despite its sensitivity to radiation and chemotherapy, NPC has a high propensity for recurrence and metastasis. Although lymph node levels have been indicated as an independent prognostic factor for NPC, there has been no precise prognostic biomarker to predict clinical outcomes for NPC before advanced disease. In the present study, we surveyed differentially expressed genes in NPC via the next-generation sequencing (NGS)-based Oncomine database and identified the spindlin family member 4 (SPIN4) gene as the most relevant to advanced nodal status. We collected 124 tumor samples from NPC patients receiving biopsy, and the expression level of SPIN4 was evaluated by immunohistochemistry. The results showed that tumors with high SPIN4 expression were significantly correlated with advanced nodal status (p < 0.001) and advanced AJCC stages (p < 0.001). High SPIN4 expression in tumor samples was an unfavorable prognostic factor for all three endpoints at the univariate level: disease-specific survival (DSS), distal metastasis-free survival (DMeFS), and local recurrence-free survival (LRFS) (all p < 0.05). High SPIN4 expression remained independently prognostic of worse DMeFS (p = 0.049) at the multivariate level. Using bioinformatics analysis, we further found that high SPIN4 level may link tight junctions to cancer cell survival. Collectively, these results imply that high SPIN4 expression is linked to an aggressive clinical course, including advanced nodal status and poor survival in NPC patients, emphasizing the promising prognostic utility of SPIN4 expression.
Specification of Environmental Consequences of the Life Cycle of Selected Post-Production Waste of Wind Power Plants Blades
Authors: Piotrowska Katarzyna , Piasecka Izabela
Published: September 2021

Wind power plants during generation of electricity emit almost no detrimental substances into the milieu. Nonetheless, the procedure of extraction of raw materials, production of elements and post-use management carry many negative environmental consequences. Wind power plant blades are mainly made of polymer materials, which cause a number of problems during post-use management. Controlling the system and the environment means such a transformation of their inputs in time that will ensure the achievement of the goal of this system or the state of the environment. Transformations of control of system and environment inputs, for example, blades production, are describing various models which in the research methodology, like LCA (Life Cycle Assessment), LCM (Life Cycle Management), LCI (Life Cycle Inventory), etc. require meticulous grouping and weighing of life cycle variables of polymer materials. The research hypothesis was assuming, in this paper, that the individual post-production waste of wind power plant blades is characterized by a different potential impact on the environment. For this reason, the aim of this publication is to conduct an ecological and energy life cycle analysis, evaluation, steering towards minimization and development (positive progress) of selected polymer waste produced during the manufacture of wind power plant blades. The analyzes were based on the LCA method. The subject of the research was eight types of waste (fiberglass mat, roving fabric, resin discs, distribution hoses, spiral hoses with resin, vacuum bag film, infusion materials residues and surplus mater), which are most often produced during the production of blades. Eco-indicator 99 and CED (Cumulative Energy Demand) were used as the computation procedures. The influence of the analyzed objects on human health, ecosystem quality and resources was appraised. Amidst the considered wastes, the highest level of depreciating impact on the milieu was found in the life cycle of resin discs (made of epoxy resin). The application of recycling processes would decrease the depreciating environmental influence in the context of the total life cycle of all analyzed waste. Based on the outcome of the analyzes, recommendations were proposed for the environmentally friendly post-use management of wind power plant blades, that can be used to develop new blade manufacturing techniques that better fit in with sustainable development and the closed-cycle economy.
The Influence of Iron Ions on Optical Brighteners and Their Application to Cotton Fabrics
Authors: Dekanić Tihana , Pušić Tanja , Soljačić Ivo , Vojnović Branka , Valh Julija Volmajer
Published: September 2021

The influence of iron ions at concentrations of 0.2, 0.5, and 1.0 g/L on optical brighteners of the groups stilbene and biphenyl in solution and on cotton fabric was investigated. Both groups of optical brighteners are intended for detergent formulations. The influence of iron ions was studied by absorption and fluorescence spectra in solution and by whiteness degree, identifying color differences using CIEL*a*b* coordinates and Ultraviolet Protection Factor (UPF) of cotton fabrics. The obtained results in solutions and cotton fabrics showed different behavior of optical brighteners stilbene and biphenyl in the presence of iron. Stilbene compounds with metal ions produced new species capable of absorbing in the UV-B region of the spectrum. A biphenyl compound in combination with iron had no effect on the absorption properties. Both optical brighteners were influenced by iron ions in the sense of fluorescence quenching. The influence of iron ions in single- and two-bath treatments of cotton fabrics after one cycle on whiteness degree and UPF was negligible.
On a Special Weighted Version of the Odd Weibull-Generated Class of Distributions
Authors: Mi Zichuan , Hussain Saddam , Chesneau Christophe
Published: September 2021

In recent advances in distribution theory, the Weibull distribution has often been used to generate new classes of univariate continuous distributions. They find many applications in important disciplines such as medicine, biology, engineering, economics, informatics, and finance; their usefulness is synonymous with success. In this study, a new Weibull-generated-type class is presented, called the weighted odd Weibull generated class. Its definition is based on a cumulative distribution function, which combines a specific weighted odd function with the cumulative distribution function of the Weibull distribution. This weighted function was chosen to make the new class a real alternative in the first-order stochastic sense to two of the most famous existing Weibull generated classes: the Weibull-G and Weibull-H classes. Its mathematical properties are provided, leading to the study of various probabilistic functions and measures of interest. In a consequent part of the study, the focus is on a special three-parameter survival distribution of the new class defined with the standard exponential distribution as a reference. The exploratory analysis reveals a high level of adaptability of the corresponding probability density and hazard rate functions; the curves of the probability density function can be decreasing, reversed N shaped, and unimodal with heterogeneous skewness and tail weight properties, and the curves of the hazard rate function demonstrate increasing, decreasing, almost constant, and bathtub shapes. These qualities are often required for diverse data fitting purposes. In light of the above, the corresponding data fitting methodology has been developed; we estimate the model parameters via the likelihood function maximization method, the efficiency of which is proven by a detailed simulation study. Then, the new model is applied to engineering and environmental data, surpassing several generalizations or extensions of the exponential model, including some derived from established Weibull-generated classes; the Weibull-G and Weibull-H classes are considered. Standard criteria give credit to the proposed model; for the considered data, it is considered the best.
Docosahexaenoic Acid-Acylated Astaxanthin Esters Exhibit Superior Renal Protective Effect to Recombination of Astaxanthin with DHA via Alleviating Oxidative Stress Coupled with Apoptosis in Vancomycin-Treated Mice with Nephrotoxicity
Authors: Shi Hao-Hao , Guo Ying , Chen Li-Pin , Wang Cheng-Cheng , Huang Qing-Rong , Xue Chang-Hu , Wang Yu-Ming , Zhang Tian-Tian
Published: September 2021

Prevention of acute kidney injury caused by drugs is still a clinical problem to be solved urgently. Astaxanthin (AST) and docosahexaenoic acid (DHA) are important marine-derived active ingredients, and they are reported to exhibit renal protective activity. It is noteworthy that the existing forms of AST in nature are mainly fatty acid-acylated AST monoesters and diesters, as well as unesterified AST, in which DHA is an esterified fatty acid. However, no reports focus on the different bioactivities of unesterified AST, monoesters and diesters, as well as the recombination of DHA and unesterified AST on nephrotoxicity. In the present study, vancomycin-treated mice were used to evaluate the effects of DHA-acylated AST monoesters, DHA-acylated AST diesters, unesterified AST, and the recombination of AST and DHA in alleviating nephrotoxicity by determining serum biochemical index, histopathological changes, and the enzyme activity related to oxidative stress. Results found that the intervention of DHA-acylated AST diesters significantly ameliorated kidney dysfunction by decreasing the levels of urea nitrogen and creatinine, alleviating pathological damage and oxidative stress compared to AST monoester, unesterified AST, and the recombination of AST and DHA. Further studies revealed that dietary DHA-acylated AST esters could inhibit the activation of the caspase cascade and MAPKs signaling pathway, and reduce the levels of pro-inflammatory cytokines. These findings indicated that the administration of DHA-acylated AST esters could alleviate vancomycin-induced nephrotoxicity, which represented a potentially novel candidate or therapeutic adjuvant for alleviating acute kidney injury.
Structure-Property Relationship on the Example of Gas Separation Characteristics of Poly(Arylene Ether Ketone)s and Poly(Diphenylene Phtalide)
Authors: Alentiev Alexandre , Chirkov Sergey , Nikiforov Roman , Buzin Mikhail , Miloserdov Oleg , Ryzhikh Victoria , Belov Nikolay , Shaposhnikova Vera , Salazkin Sergey
Published: September 2021

Three poly(arylene ether ketone)s (PAEKs) with propylidene (C1, C2) and phtalide (C3) fragments, and one phtalide-containing polyarylene (C4), were synthesized. Their chemical structures were confirmed via 1H NMR, 13C NMR and 19F NMR spectroscopy. The polymers have shown a high glass transition temperature (>155 °C), excellent film-forming properties, and a high free volume for this polymer type. The influence of various functional groups in the structure of PAEKs was evaluated. Expectedly, due to higher free volume the introduction of hexafluoropropylidene group to PAEK resulted in higher increase of gas permeability in comparison with propylidene group. The substitution of the fluorine-containing group on a rigid phtalide moiety (C3) significantly increases glass transition temperature of the polymer while gas permeation slightly decreases. Finally, the removal of two ether groups from PAEK structure (C4) leads to a rigid polymer chain that is characterized by highest free volume, gas permeability and diffusion coefficients among the PAEKs under investigation. Methods of modified atomic (MAC) and bond (BC) contributions were applied to estimate gas permeation and diffusion. Both techniques showed reasonable predicted parameters for three polymers while a significant underestimation of gas transport parameters was observed for C4. Gas solubility coefficients for PAEKs were forecasted by “Short polymer chain surface based pre-diction” (SPCSBP) method. Results for all three prediction methods were compared with the ex-perimental data obtained in this work. Predicted parameters were in good agreement with ex-perimental data for phtalide-containing polymers (C3 and C4) while for propylidene-containing poly(arylene ether ketone)s they were overestimated due to a possible influence of propylidene fragment on indices of oligomeric chains. MAC and BC methods demonstrated better prediction power than SPCSBP method.
Engineered Membranes for Residual Cell Trapping on Microfluidic Blood Plasma Separation Systems: A Comparison between Porous and Nanofibrous Membranes
Authors: Lopresti Francesco , Keraite Ieva , Ongaro Alfredo Edoardo , Howarth Nicola Marie , La Carrubba Vincenzo , Kersaudy-Kerhoas Maïwenn
Published: September 2021

Blood-based clinical diagnostics require challenging limit-of-detection for low abundance, circulating molecules in plasma. Micro-scale blood plasma separation (BPS) has achieved remarkable results in terms of plasma yield or purity, but rarely achieving both at the same time. Here, we proposed the first use of electrospun polylactic-acid (PLA) membranes as filters to remove residual cell population from continuous hydrodynamic-BPS devices. The membranes hydrophilicity was improved by adopting a wet chemistry approach via surface aminolysis as demonstrated through Fourier Transform Infrared Spectroscopy and Water Contact Angle analysis. The usability of PLA-membranes was assessed through degradation measurements at extreme pH values. Plasma purity and hemolysis were evaluated on plasma samples with residual red blood cell content (1, 3, 5% hematocrit) corresponding to output from existing hydrodynamic BPS systems. Commercially available membranes for BPS were used as benchmark. Results highlighted that the electrospun membranes are suitable for downstream residual cell removal from blood, permitting the collection of up to 2 mL of pure and low-hemolyzed plasma. Fluorometric DNA quantification revealed that electrospun membranes did not significantly affect the concentration of circulating DNA. PLA-based electrospun membranes can be combined with hydrodynamic BPS in order to achieve high volume plasma separation at over 99% plasma purity.
Ceramic Cutting Materials and Tools Suitable for Machining High-Temperature Nickel-Based Alloys: A Review
Authors: Seleznev Anton , Pinargote Nestor Washington Solis , Smirnov Anton
Published: September 2021

Nickel-based superalloys are attractive to many industrial sectors (automotive, military, energy, aerospace, etc.). However, their physical properties make them difficult to machining using traditional tools. Therefore, new materials for the machining of Ni-based alloys are required. Ceramic-based composites could act as a tool to replace the current materials. The incentives for this paper are to provide an overview of existing ceramic composites and draw some conclusions that will help in solving the problem of choosing materials for the processing of Ni-based superalloys. Despite the diversity of ceramic composites in this work, the possibility of using the SiAlON ceramic for Ni-based alloy machining is highlighted.
Positioning Performance of a Sub-Arc-Second Micro-Drive Rotary System
Authors: Yang Manzhi , Lv Zhenyang , Zhang Chuanwei , Yang Yizhi , Jing Gang , Guo Wei , Lu Zhengxiong , Huang Yumei , Wei Kaiyang , Li Linyue , Feng Bin , Ge Hongyu
Published: September 2021

In the macro/micro dual-drive rotary system, the micro-drive system compensates for the position error of the macro-drive system. To realize the sub-arc-second (i.e., level of 1″–0.1″) positioning of the macro/micro dual-drive rotary system, it is necessary to study the positioning performance of the sub-arc-second micro-drive rotary system. In this paper, we designed a sub-arc-second micro-drive rotary system consisting of a PZT (piezoelectric actuator) and a micro rotary mechanism, and used simulation and experimental methods to study the positioning performance of the system. First, the micro-drive rotary system was developed to provide ultra-precise rotary motion. In this system, the PZT has ultrahigh resolution at a level of 0.1 nanometers in linear motion; a micro rotating mechanism was designed according to the composite motion principle of the flexible hinge, which could transform the linear motion of piezoelectric ceramics into rotating motion accurately. Second, the drive performance was analyzed based on the drive performance experiment. Third, kinematics, simulation, and experiments were carried out to analyze the transformation performance of the system. Finally, the positioning performance equation of the system was established based on the two performance equations, and the maximum rotary displacements and positioning error of the system were calculated. The study results showed that the system can provide precision motion at the sub-arc-second and good linearity of motion. This study has a certain reference value in ultra-precision positioning and micromachining for research on rotary motion systems at the sub-arc-second level.
Anisotropic Optical Response of WTe2 Single Crystals Studied by Ellipsometric Analysis
Authors: Buchkov Krastyo , Todorov Rosen , Terziyska Penka , Gospodinov Marin , Strijkova Velichka , Dimitrov Dimitre , Marinova Vera
Published: September 2021

In this paper we report the crystal growth conditions and optical anisotropy properties of Tungsten ditelluride (WTe2) single crystals. The chemical vapor transport (CVT) method was used for the synthesis of large WTe2 crystals with high crystallinity and surface quality. These were structurally and morphologically characterized by means of X-ray diffraction, optical profilometry and Raman spectroscopy. Through spectroscopic ellipsometry analysis, based on the Tauc–Lorentz model, we identified a high refractive index value (~4) and distinct tri-axial anisotropic behavior of the optical constants, which opens prospects for surface plasmon activity, revealed by the dielectric function. The anisotropic physical nature of WTe2 shows practical potential for low-loss light modulation at the 2D nanoscale level.
In Situ Collection of Nanoparticles during Femtosecond Laser Machining in Air
Authors: Joy Nithin , Kietzig Anne-Marie
Published: September 2021

Nanoparticles generated during laser material processing are often seen as annoying side products, yet they might find useful application upon proper collection. We present a parametric study to identify the dominant factors in nanoparticle removal and collection with the goal of establishing an in situ removal method during femtosecond laser machining. Several target materials of different electrical resistivity, such as Cu, Ti, and Si were laser machined at a relatively high laser fluence. Machining was performed under three different charge conditions, i.e., machining without an externally applied charge (alike atmospheric pulsed laser deposition (PLD)) was compared to machining with a floating potential and with an applied field. Thereby, we investigated the influence of three different charge conditions on the behavior of laser-generated nanoparticles, in particular considering plume deflection, nanoparticle accumulation on a collector plate and their redeposition onto the target. We found that both strategies, machining under a floating potential or under an applied field, were effective for collecting laser-generated nanoparticles. The applied field condition led to the strongest confinement of the nanoparticle plume and tightest resulting nanoparticle collection pattern. Raster-scanning direction was found to influence the nanoparticle collection pattern and ablation depth. However, the laser-processed target surface remained unaffected by the chosen nanoparticle collection strategy. We conclude that machining under a floating potential or an applied field is a promising setup for removing and collecting nanoparticles during the machining process, and thus provides an outlook to circular waste-free laser process design.
Improvement of Tardive Dyskinesia during Mindfulness Meditation
Authors: Santoro Maria Angela , English Isolde , Sezer Idil , Amagat Mickael , Ly Frank , Chaneac Edouard , Cailliez Patricia , Bottemanne Hugo
Published: September 2021

Background: We report the case of a patient presenting with orofacial tardive dyskinesia (TD), following administration of a first-generation antipsychotic (Loxapine). Intervention: Four weeks of repeated sessions of mindfulness-based cognitive therapy (MBCT) and mindfulness-based stress reduction (MBSR) protocols were administered, with TD hetero-quantified before and during each session via the Abnormal Involuntary Movement Scale (AIMS). Results: The dyskinesia ameliorated quantitatively and qualitatively (1) during each session, and (2) at resting conditions in the long term. During some sessions, after which patients’ compliance was auto-evaluated as maximal, complete arrest of the TD was observed. Hypothesis and Conclusion: We suggest mindfulness meditation as a novel adjunctive therapeutic approach for tardive dyskinesia, and invite for further clinical and neurological investigations.
Nutritional Status of Adolescent Afghan Refugees Living in Peshawar, Pakistan
Authors: Saeedullah Anum , Khan Muhammad Shabir , Andrews Simon C. , Iqbal Khalid , Ul-Haq Zia , Qadir Syed Abdul , Khan Haris , Iddrisu Ishawu , Shahzad Muhammad
Published: September 2021

Pakistan has hosted millions of Afghan refugees over the last several decades. Due to poor socioeconomic status, food insecurity and inadequate access to health care, these refugees are considered to be at high risk of malnutrition. Previous studies on nutritional assessment of high-risk populations (refugees) have focused mainly on women and children (0–59 months). The current study aims to assess nutritional status of adolescent Afghan refugees; the population who are equally vulnerable to malnutrition and its consequences. In this cross sectional study, the nutritional status of 206 adolescent (10–19 years old) Afghans boys and girls living in a refugee camp in Peshawar, Pakistan was assessed using standard methods. The results indicate a prevalence of stunting, thinness, and overweight and obesity at 35.3%, 4.4% and 14.8%, respectively. Furthermore, there was a significantly high prevalence of micronutrient deficiencies (vitamin D, 80.5%; vitamin B12, 41.9%; and folate, 28.2%); and anemia (10.1%). Together, these findings indicate that this vulnerable population group suffers from the double burden of malnutrition and are thus at serious risk of impaired psychosocial cognitive development, general ill-health and diminished wellbeing. This study therefore highlights the urgent need to include adolescents in regular screening and intervention programs of such at-risk populations.
Brugada Pattern in a Child with Severe SARS-CoV-2 Related Multisystem Inflammatory Syndrome
Authors: De Nigris Angelica , Pepe Angela , Di Nardo Giangiacomo , Giannattasio Antonietta , Pagano Annamaria , D’Anna Carolina , Muzzica Stefania , Lenta Selvaggia , Di Marco Giovanni Maria , Tipo Vincenzo
Published: September 2021

This report presents the first case of Brugada pattern complicated by a supraventricular arrhythmia in a child with SARS-CoV-2 related Multisystem Inflammatory Syndrome in Children (MIS-C). A 7-year-old boy came to our Emergency Department with 7 days of abdominal pain and fever. MIS-C was diagnosed on the basis of the clinical, laboratory and instrumental tests. On admission, ECG showed type 1 Brugada pattern in the right precordial leads. During hospitalization the onset of supraventricular arrhythmias complicated the clinical picture. This case underlines management complexity of supraventricular arrhythmic events, different from atrial fibrillation, in patients with Brugada pattern in the context of a systemic inflammatory condition with significant cardiac involvement. All potential therapeutic choices should be considered to ensure the best outcomes.
Comparison of Sympathetic Skin Response (SSR) between Electrical and Acoustic Stimuli in a Healthy Pediatric Population
Authors: Cavaliere Sara , Bertini Giovanna , Cossu Cesarina , Bastianelli Maria , Gabbanini Simonetta , Mei Cristina , Lori Silvia
Published: September 2021

Data in the literature report that latency and morphology in the cutaneous sympathetic skin response (SSR) do not change according to the type of stimulus delivered, unlike the amplitude which shows greater values in relation to the intensity of the physical impact caused in patient. Since the acoustic stimulus represents a method better tolerated by the pediatric patient, the aim of this study is to evaluate the presence or absence of significant differences in SSR between electrical and acoustic stimuli. The SSR was performed for each child of 18 recruited in this study, deriving from the palm of the hand and the sole of the foot and initially delivering an electrical stimulus at the level of the median nerve at the wrist. Two acoustic stimuli were subsequently delivered with the aid of audiometric headphones. Our results show no significant differences for the amplitude values obtained (p values > 0.05). For the latency there was a statistically significant difference (p-value = 0.001) for the left hand, subsequently not confirmed by the comparison performed between the two sides (p-values = 0.28 and 0.56). If these preliminary data are confirmed by a larger sample, the acoustic stimulus could be introduced in a standardized protocol for performing SSR in pediatric patients.
Childhood Obesity and Maternal Personality Traits: A New Point of View on Obesity Behavioural Aspects
Authors: Precenzano Francesco , Smirni Daniela , Vetri Luigi , Marzuillo Pierluigi , Lanzara Valentina , Bitetti Ilaria , Siciliano Margherita , Miraglia del Giudice Emanuele , Esposito Maria , Santoro Nicola , Carotenuto Marco
Published: September 2021

The epidemic spread of childhood obesity in Western society has interested many researchers, who agree in defining it as a multifactorial disease in which not only eating habits and sedentary lifestyle play a role, but also genetic predisposition. The aim of this study was to analyze the personality profile of a group of mothers of children with obesity and to compare this profile to that of a group of mothers of children without obesity. A total of 258 mothers participated in the study (126 mothers of children with obesity and 132 mothers of children without obesity). Weight and height were measured and the body mass index was calculated. The Minnesota Multiphasic Personality Inventory second edition (MMPI-2), evaluating personality and psychological disorders, was used to evaluate the personality profile. The results suggested that mothers of children with obesity score higher than the mothers of children without obesity in all MMPI-2 subscales. In most of these subscales, the differences between the two groups of mothers were statistically significant and with a medium to high effect size. These data suggest a new perspective on childhood obesity, identifying it as a multifactorial pathology that requires a multimodal and multidisciplinary approach that also takes care of caregivers to ensure optimal therapeutic efficacy.
Multi-Target Actions of Acridones from Atalantia monophylla towards Alzheimer’s Pathogenesis and Their Pharmacokinetic Properties
Authors: Takomthong Pitchayakarn , Waiwut Pornthip , Yenjai Chavi , Sombatsri Aonnicha , Reubroycharoen Prasert , Lei Luo , Lai Ren , Chaiwiwatrakul Suchada , Boonyarat Chantana
Published: September 2021

Ten acridones isolated from Atalantia monophylla were evaluated for effects on Alzheimer’s disease pathogenesis including antioxidant effects, acetylcholinesterase (AChE) inhibition, prevention of beta-amyloid (Aβ) aggregation and neuroprotection. To understand the mechanism, the type of AChE inhibition was investigated in vitro and binding interactions between acridones and AChE or Aβ were explored in silico. Drug-likeness and ADMET parameters were predicted in silico using SwissADME and pKCSM programs, respectively. All acridones showed favorable drug-likeness and possessed multifunctional activities targeting AChE function, Aβ aggregation and oxidation. All acridones inhibited AChE in a mixed-type manner and bound AChE at both catalytic anionic and peripheral anionic sites. In silico analysis showed that acridones interfered with Aβ aggregation by interacting at the central hydrophobic core, C-terminal hydrophobic region, and the key residues 41 and 42. Citrusinine II showed potent multifunctional action with the best ADMET profile and could alleviate neuronal cell damage induced by hydrogen peroxide and Aβ1-42 toxicity.
A Role of BDNF in the Depression Pathogenesis and a Potential Target as Antidepressant: The Modulator of Stress Sensitivity “Shati/Nat8l-BDNF System” in the Dorsal Striatum
Authors: Miyanishi Hajime , Nitta Atsumi
Published: September 2021

Depression is one of the most common mental diseases, with increasing numbers of patients globally each year. In addition, approximately 30% of patients with depression are resistant to any treatment and do not show an expected response to first-line antidepressant drugs. Therefore, novel antidepressant agents and strategies are required. Although depression is triggered by post-birth stress, while some individuals show the pathology of depression, others remain resilient. The molecular mechanisms underlying stress sensitivity remain unknown. Brain-derived neurotrophic factor (BDNF) has both pro- and anti-depressant effects, dependent on brain region. Considering the strong region-specific contribution of BDNF to depression pathogenesis, the regulation of BDNF in the whole brain is not a beneficial strategy for the treatment of depression. We reviewed a novel finding of BDNF function in the dorsal striatum, which induces vulnerability to social stress, in addition to recent research progress regarding the brain regional functions of BDNF, including the prefrontal cortex, hippocampus, and nucleus accumbens. Striatal BDNF is regulated by Shati/Nat8l, an N-acetyltransferase through epigenetic regulation. Targeting of Shati/Nat8l would allow BDNF to be striatum-specifically regulated, and the striatal Shati/Nat8l-BDNF pathway could be a promising novel therapeutic agent for the treatment of depression by modulating sensitivity to stress.
Targeted Photodynamic Therapy Using Alloyed Nanoparticle-Conjugated 5-Aminolevulinic Acid for Breast Cancer
Authors: Montaseri Hanieh , Kruger Cherie Ann , Abrahamse Heidi
Published: September 2021

Photodynamic therapy (PDT) has been investigated as an effective, non-invasive, and alternative tumor-ablative therapy that uses photosensitizers (PSs) and safe irradiation light in the presence of oxygen to generate reactive oxygen species (ROS) to kill malignant cancer cells. However, the off-target activation of the PSs can hinder effective PDT. Therefore, an advanced drug delivery system is required to selectively deliver the PS to the therapeutic region only and reduce off-target side effects in cancer treatment. The integration of laser-initiated PDT with nanotechnology has provided new opportunities in cancer therapy. In this study, plasmonic bimetallic nanoparticles (NPs) were prepared for the targeted PDT (TPDT) of in vitro cultured MCF-7 breast cancer cells. The NPs were functionalized with PEG through Au–thiol linkage to enhance their biocompatibility and subsequently attached to the PS precursor 5-aminolevulinic acid via electrostatic interactions. In order to enhance specific targeting, anti-HER-2 antibodies (Ab) were decorated onto the surface of the nanoconjugate (NC) to fabricate a 5-ALA/Au–Ag-PEG-Ab NC. In vitro studies showed that the synthesized NC can enter MCF-7 cells and localize in the cytoplasm to metabolize 5-ALA to protoporphyrin IX (PpIX). Upon light irradiation, PpIX can efficiently produce ROS for the PDT treatment of MCF-7. Cellular viability studies showed a decrease from 49.8% ± 5.6 ** to 13.8% ± 2.0 *** for free 5-ALA versus the NC, respectively, under equivalent concentrations of the PS (0.5 mM, IC50). These results suggest that the active targeted NC platform has an improved PDT effect on MCF-7 breast cancer cells.
From Oxidative Stress to Inflammation in the Posterior Ocular Diseases: Diagnosis and Treatment
Authors: Dammak Azza , Huete-Toral Fernando , Carpena-Torres Carlos , Martin-Gil Alba , Pastrana Cristina , Carracedo Gonzalo
Published: September 2021

Most irreversible blindness observed with glaucoma and retina-related ocular diseases, including age-related macular degeneration and diabetic retinopathy, have their origin in the posterior segment of the eye, making their physiopathology both complex and interconnected. In addition to the age factor, these diseases share the same mechanism disorder based essentially on oxidative stress. In this context, the imbalance between the production of reactive oxygen species (ROS) mainly by mitochondria and their elimination by protective mechanisms leads to chronic inflammation. Oxidative stress and inflammation share a close pathophysiological process, appearing simultaneously and suggesting a relationship between both mechanisms. The biochemical end point of these two biological alarming systems is the release of different biomarkers that can be used in the diagnosis. Furthermore, oxidative stress, initiating in the vulnerable tissue of the posterior segment, is closely related to mitochondrial dysfunction, apoptosis, autophagy dysfunction, and inflammation, which are involved in each disease progression. In this review, we have analyzed (1) the oxidative stress and inflammatory processes in the back of the eye, (2) the importance of biomarkers, detected in systemic or ocular fluids, for the diagnosis of eye diseases based on recent studies, and (3) the treatment of posterior ocular diseases, based on long-term clinical studies.