bluuebunny/crossref_metadata_2025 huggingface dataset:
Gastrocnemius Medialis Muscle Geometry and Extensibility in Typically Developing Children and Children With Spastic Paresis Aged 6–13 Years
Authors: Weide Guido , Huijing Peter A. , Bar-On Lynn , Sloot Lizeth , Buizer Annemieke I. , Becher Jules G. , Harlaar Jaap , Jaspers Richard T.
Published: November 2020

Gait of children with spastic paresis (SP) is frequently characterized by a reduced ankle range of motion, presumably due to reduced extensibility of the triceps surae (TS) muscle. Little is known about how morphological muscle characteristics in SP children are affected. The aim of this study was to compare gastrocnemius medialis (GM) muscle geometry and extensibility in children with SP with those of typically developing (TD) children and assess how GM morphology is related to its extensibility. Thirteen children with SP, of which 10 with a diagnosis of spastic cerebral palsy and three with SP of unknown etiology (mean age 9.7 ± 2.1 years; GMFCS: I–III), and 14 TD children (mean age 9.3 ± 1.7 years) took part in this study. GM geometry was assessed using 3D ultrasound imaging at 0 and 4 Nm externally imposed dorsal flexion ankle moments. GM extensibility was defined as its absolute length change between the externally applied 0 and 4 Nm moments. Anthropometric variables and GM extensibility did not differ between the SP and TD groups. While in both groups, GM muscle volume correlated with body mass, the slope of the regression line in TD was substantially higher than that in SP (TD = 3.3 ml/kg; SP = 1.3 ml/kg, p < 0.01). In TD, GM fascicle length increased with age, lower leg length and body mass, whereas in SP children, fascicle length did not correlate with any of these variables. However, the increase in GM physiological cross-sectional area as a function of body mass did not differ between SP and TD children. Increases in lengths of tendinous structures in children with SP exceeded those observed in TD children (TD = 0.85 cm/cm; SP = 1.16 cm/cm, p < 0.01) and even exceeded lower-leg length increases. In addition, only for children with SP, body mass (r = −0.61), height (r = −0.66), muscle volume (r = − 0.66), physiological cross-sectional area (r = − 0.59), and tendon length (r = −0.68) showed a negative association with GM extensibility. Such negative associations were not found for TD children. In conclusion, physiological cross-sectional area and length of the tendinous structures are positively associated with age and negatively associated with extensibility in children with SP.
SHP-2-Induced Activation of c-Myc Is Involved in PDGF-B-Regulated Cell Proliferation and Angiogenesis in RMECs
Authors: Ma Jun , Tang Wenyi , Gu Ruiping , Hu Fangyuan , Zhang Lei , Wu Jihong , Xu Gezhi
Published: November 2020

Background: Aberrant neovascularization resulting from inappropriate angiogenic signaling is closely related to many diseases, such as cancer, cardiovascular disease, and proliferative retinopathy. Although some factors involved in regulating pathogenic angiogenesis have been identified, the molecular mechanisms of proliferative retinopathy remain largely unknown. In the present study, we determined the role of platelet-derived growth factor-B (PDGF-B), one of the HIF-1-responsive gene products, in cell proliferation and angiogenesis in retinal microvascular endothelial cells (RMECs) and explored its regulatory mechanism.

Methods: Cell counting kit-8 (CCK-8), bromodeoxyuridine (BrdU) incorporation, tube formation, cell migration, and Western blot assays were used in our study.

Results: Our results showed that PDGF-B promoted cell proliferation and angiogenesis by increasing the activity of Src homology 2 domain-containing tyrosine phosphatase 2 (SHP-2) in RMECs, which was attenuated by the inhibition of PDGF receptor (PDGFR) or SHP-2 knockdown. Moreover, activation of c-Myc was involved in the processes of PDGF-B/SHP-2-driven cell proliferation in RMECs. The promoting effects of PDGF-B/SHP-2 on c-Myc expression were mediated by the Erk pathway.

Conclusion: These results indicate that PDGF-B facilitates cell proliferation and angiogenesis, at least in part, via the SHP-2/Erk/c-Myc pathway in RMECs, implying new potential treatment candidates for retinal microangiopathy.
The Joint Effect of a Combination of Components From the Fruit of Crataegus pinnatifida Bge. Var. major N.E. Br. and the Root of Salvia miltiorrhiza Bge. With Exercises on Swimming in Focal Cerebral Infraction in Rat
Authors: Ding Shilan , Wang Wei , Yin Xiaojie , Wang Lan , Gong Leilei , Liao Fulong , Liang Rixin
Published: November 2020

Background: In our previous study, we found that the combination of a traditional Chinese medicine (TCM) and swimming could prevent atherosclerosis through a synergistic interaction. However, whether the combined application of active components from the fruit of Crataegus pinnatifida Bge. Var. major N.E. Br. and the root of Salvia miltiorrhiza Bge. (CPSM) and swimming has been effective in the prevention and treatment of focal cerebral infraction remained unclear. This work aimed to conduct detailed investigation on the joint effects of CPSM extract with swimming on focal cerebral infraction in rats and its underlying mechanisms.

Method: A photochemical method of the combination of Rose Bengal (RB) injection and cold-light source irradiation was performed to establish the rat focal cerebral thrombosis model. The pathological changes of the brain were observed by a DCP-7030 laser multifunction machine, and the protein levels of von Willebrand factor (vWF), vascular cell adhesion molecule-1 (VCAM-1), and intercellular adhesion molecule-1 (ICAM-1) were detected by Western blotting. Blood samples were collected to assay tissue plasminogen activator (t-PA), plasminogen activator inhibitor type-1 (PAI-1), endothelin-1 (ET-1), 6-keto-prostaglandin F1α (6-keto-PGF1α), and thromboxane B2 (TXB2). Finally, the gene expression of t-PA, PAI-1, and ICAM-1 in human umbilical vein endothelial cells (HUVECs) stimulated by tumor necrosis factor-α (TNF-α) was assayed via real-time (RT) quantitative PCR (qPCR).

Results: The joint effects of CPSM extract and swimming demonstrated significant interactions, which including increased blood perfusion, increased serum t-PA and 6-keto-PGF1α, decreased serum PAI-1 and TXB2, decreased protein levels of vWF, VCAM-1 and ICAM-1, and decreased ICAM-1 gene expression.

Conclusion: This research demonstrated that the combined therapy of CP and SM extracts with swimming could prevent focal cerebral infraction through interactions on the regulation of vascular endothelial functions and inflammatory factors. It stresses the promising effects of the drugs and shear stress of blood flow in prevention and treatment of thrombosis. The mechanism may be related to regulating the protein expression of vWF, VCAM-1, and ICAM-1, and downregulating the gene expression of ICAM-1.
Exosomal MiR-769-5p Exacerbates Ultraviolet-Induced Bystander Effect by Targeting TGFBR1
Authors: Ni Na , Ma Weiwei , Tao Yanling , Liu Juan , Hua Hui , Cheng Jiawei , Wang Jie , Zhou Bingrong , Luo Dan
Published: November 2020

Exosomal microRNAs have been investigated in bystander effect, but it is unclear whether microRNA works in ultraviolet radiation–induced bystander effects (UV-RIBEs) and what the underlying mechanism could be. Exosomes from ultraviolet (UV)–irradiated human skin fibroblasts (HSFs) were isolated and transferred to normal HSFs, followed by the detection of proliferation rate, oxidative damage level, and apoptosis rate. Exosomal miRNAs were evaluated and screened with miRNA sequencing and quantitative reverse transcriptase–polymerase chain reaction method. MiRNA shuttle and bystander photodamage reactions were observed after transfection of miR-769-5p. MiR-769-5p targeting gene transforming growth factor-β1 (TGFBR1), and TGFBR1 mRNA 3′-untranslated region (UTR) was assessed and identified by Western blotting and dual-luciferase reporter assay. Bystander effects were induced after being treated with isolated exosomes from UV-irradiated HSFs. Exosomal miR-769-5p expression was significantly upregulated. Human skin fibroblasts showed lower proliferation, increasing oxidative damage, and faster occurrence of apoptosis after transfection. Exosome-mediated transfer of miR-769-5p was observed. Upregulation of miR-769-5p induced bystander effects, whereas downregulation of miR-769-5p can suppress UV-RIBEs. In addition, miR-769-5p was found to downregulate TGFBR1 gene expression by directly targeting its 3′-UTR. Our results demonstrate that exosome-mediated miR-769-5p transfer could function as an intercellular messenger and exacerbate UV-RIBEs. MiR-769-5p inhibits the expression of TGFBR1 by targeting TGFBR1 mRNA 3′-UTR.
Changes in Liver Mechanical Properties and Water Diffusivity During Normal Pregnancy Are Driven by Cellular Hypertrophy
Authors: Garczyńska Karolina , Tzschätzsch Heiko , Kühl Anja A. , Morr Anna Sophie , Lilaj Ledia , Häckel Akvile , Schellenberger Eyk , Berndt Nikolaus , Holzhütter Hermann-Georg , Braun Jürgen , Sack Ingolf , Guo Jing
Published: November 2020

During pregnancy, the body’s hyperestrogenic state alters hepatic metabolism and synthesis. While biochemical changes related to liver function during normal pregnancy are well understood, pregnancy-associated alterations in biophysical properties of the liver remain elusive. In this study, we investigated 26 ex vivo fresh liver specimens harvested from pregnant and non-pregnant rats by diffusion-weighted imaging (DWI) and magnetic resonance elastography (MRE) in a 0.5-Tesla compact magnetic resonance imaging (MRI) scanner. Water diffusivity and viscoelastic parameters were compared with histological data and blood markers. We found livers from pregnant rats to have (i) significantly enlarged hepatocytes (26 ± 15%, p < 0.001), (ii) increased liver stiffness (12 ± 15%, p = 0.012), (iii) decreased viscosity (−23 ± 14%, p < 0.001), and (iv) increased water diffusivity (12 ± 11%, p < 0.001). In conclusion, increased stiffness and reduced viscosity of the liver during pregnancy are mainly attributable to hepatocyte enlargement. Hypertrophy of liver cells imposes fewer restrictions on intracellular water mobility, resulting in a higher hepatic water diffusion coefficient. Collectively, MRE and DWI have the potential to inform on structural liver changes associated with pregnancy in a clinical context.
Development and Psychometric Properties of the Questionnaire for Assessing Educational Podcasts (QAEP)
Authors: Alarcón Rafael , Blanca María J.
Published: November 2020

The aim of this research was to develop and validate the Questionnaire for Assessing Educational Podcasts (QAEP), an instrument designed to gather students’ views about four dimensions of educational podcasts: access and use, design and structure, content adequacy, and value as an aid to learning. In study 1 we gathered validity evidence based on test content by asking a panel of experts to rate the clarity and relevance of items. Study 2 examined the psychometric properties of the QAEP, including confirmatory factor analysis with cross-validation to test the factor structure of the questionnaire, as well as item and reliability analysis. The results from study 1 showed that the experts considered the items to be clearly worded and relevant in terms of their content. The results from study 2 showed a factor structure consistent with the underlying dimensions, as well as configural and metric invariance across groups. The item analysis and internal consistency for scores on each factor and for total scores were also satisfactory. The scores obtained on the QAEP provide teachers with direct student feedback and highlight those aspects that need to be enhanced in order to improve the teaching/learning process.
Emotions and Reactions to the Confinement by COVID-19 of Children and Adolescents With High Abilities and Community Samples: A Mixed Methods Research Study
Authors: Valadez María de los Dolores , López-Aymes Gabriela , Ruvalcaba Norma Alicia , Flores Francisco , Ortíz Grecia , Rodríguez Celia , Borges África
Published: November 2020

The goal of this research is to know and compare the emotions and reactions to confinement due to the COVID-19 pandemic in children and adolescents with high abilities and community samples. This is a mixed study with an exploratory reach that is descriptive, and which combines survey and qualitative methodologies to examine the emotions and reactions to confinement experiences of children and adolescents aged between 5 and 14 years. An online poll was designed with 46 questions, grouped into three sections: (1) General Data, (2) Reactions to the health contingencies of COVID-19, and (3) Positive and negative emotions. This last section was included the Oros’ positive emotions questionnaire (2014) and the scales of the negative effect of the PANAS Schedule positive and negative affect on children and adolescents between 8 and 14 years old (Sandin, 2003). Data were collected online with a convenience sample. Quantitative data were analyzed with the SPSS statistics program and qualitative data with Alceste software. Among the main findings is the fact that there are no significant differences between the groups by ability in terms of reactions and emotions; however, there are differences between age groups and sex. The study discusses the implications for educational intervention in situations like the current pandemic.
Basque Ethnic Identity and Collective Empowerment: Two Key Factors in Well-Being and Community Participation
Authors: Zabala Jon , Conejero Susana , Pascual Aitziber , Alonso-Arbiol Itziar , Amutio Alberto , Torres-Gomez Barbara , Padoan De Luca Sonia , Telletxea Saioa
Published: November 2020

Social identity is a factor that is associated with well-being and community participation. Some studies have shown that ethnic identity goes along with empowerment, and that interaction between the two leads to greater indices of well-being and community participation. However, other works suggest a contextual circumstance (i.e., perceiving one’s own group as a minority and/or being discriminated) may condition the nature of these relations. By means of a cross-sectional study, we analyzed the relations of social identification (or identity fusion) and collective psychological empowerment with personal well-being, social well-being and community participation in a sample of Basques. A total of 748 Basques participated (63.1% women; ageM= 39.28;SD= 12.13). Individuals who were highly identified or fused with Basque speakers and who were highly empowered showed higher indices of well-being (both personal and social) and of community participation than non-fused individuals with low empowerment. The results also suggest that social identification (or identity fusion) offsets the negative effects of perceiving the group as a linguistic minority. Collective psychological empowerment proved to be an especially relevant factor that needs to continue to be explored.
A Systematic Review of the Impact of Viral Respiratory Epidemics on Mental Health: An Implication on the Coronavirus Disease 2019 Pandemic
Authors: Luo Yang , Chua Cher Rui , Xiong Zhonghui , Ho Roger C. , Ho Cyrus S. H.
Published: November 2020

Background:The twenty-first century viral respiratory epidemics have taught us valuable lessons. Our systematic review examined the impact of these epidemics, including coronavirus disease 2019 (COVID-19), on mental health among different population groups, drawing on their insights for recommendations for the current COVID-19 pandemic.

Methods:Searches were performed on PubMed, Embase, PsycINFO, Web of Science, Scopus, CINAHL, and Cochrane on April 4, 2020. Studies that had undefined mental health outcomes or did not use a validated scale for measure were excluded. Quality assessment was carried outviathe Newcastle–Ottawa Scale.

Results:We included 95 studies, most of which were conducted in Hong Kong (31.6%) and China (21.4%). A total of 30 (30.9%) studies are on the general public, 41 (42.2%) on healthcare workers, and 26 (26.6%) on patients and quarantined individuals. Furthermore, 36 (37.1%) of the studies are of high quality, 48 (49.5%) are of moderate quality, and 13 (13.4%) are of low quality. The most significant mental health outcomes reported include anxiety, depression, and post-traumatic stress disorder symptoms. The subgroups identified to have a higher risk of psychiatric symptoms among the general public include females, the elderly, individuals with chronic illness, migrant workers, and students. Long-term mental health impact was reported in some healthcare workers and epidemic patients, even up to 3 years in the former. Interestingly, when compared to non-quarantined groups, quarantine was not significantly associated with worse mental health outcomes.

Conclusion:Important implications for the COVID-19 pandemic were highlighted. Respiratory epidemics pose a significant psychological morbidity onto many population groups. Psychological support for vulnerable groups, including healthcare workers and patients, should be implemented to prevent them from spiraling into clinical psychiatric conditions.
Addressing Suicide in the Veteran Population: Engaging a Public Health Approach
Authors: Carroll David , Kearney Lisa K. , Miller Matthew A.
Published: November 2020

Suicide is a national public health issue in America, and it disproportionately affects those who are serving or who have served in the United States military. The US Department of Veterans Affairs (VA) has made suicide prevention its number one clinical priority. VA is committed to prevent suicide among the entire population of those who have served our country in the military, regardless of whether they make use of any VA services or benefits. Suicide can be prevented through the application of a public health strategy embracing partners at all levels. Following a national strategy, VA has embarked on an effort involving the application of a public health strategy combining both clinically-based and community-focused interventions. This paper describes several examples of these efforts and steps forward.
Profiles of Depressive Symptoms and Anger in Men: Associations With Postpartum Family Functioning
Authors: Macdonald Jacqui A. , Greenwood Christopher J. , Francis Lauren M. , Harrison Tessa R. , Graeme Liam G. , Youssef George J. , Di Manno Laura , Skouteris Helen , Fletcher Richard , Knight Tess , Williams Joanne , Milgrom Jeannette , Olsson Craig A.
Published: November 2020

Background: Evidence suggests that men commonly experience depression as feelings of anger; yet, research has not investigated what this means for the manifestation of depressive symptoms in the early years of fatherhood and for key indicators of family functioning.

Methods: Using data from a longitudinal cohort study of men at the normative age for entering fatherhood (28–32 years), we conducted latent class analyses to identify patterns of depressive symptoms and 3 sub-types of state anger (feeling; verbal; physical). We then assessed whether class membership was associated with paternity status (n = 535). In a subsample of fathers of infants aged up to 18 months (n = 162), we prospectively assessed associations with paternal-infant bonding, co-parenting, perceived social support, paternal involvement in childcare and alcohol use up to 2 years later.

Results: Five classes emerged that differentiated men by anger and depressive symptom severity and by the degree to which men endorsed the feeling of wanting to express anger physically. Compared to the reference class with minimal symptoms, fathers had a higher probability of being in either the mild or most severe symptom classes. Men in symptomatic classes were at higher risk of lower levels of social support, co-parenting problems, and paternal-infant bonds. Class membership was not associated with alcohol use or paternal involvement in childcare.

Conclusions: Our results reveal patterns of co-existing symptoms of depression and anger in fathers of infants that will be relevant to men's own need for support, their family safety, partner mental health and child developmental outcomes.
Mental Health Impacts of the COVID-19 Pandemic on International University Students, Related Stressors, and Coping Strategies
Authors: Lai Agnes Yuen-kwan , Lee Letitia , Wang Man-ping , Feng Yibin , Lai Theresa Tze-kwan , Ho Lai-ming , Lam Veronica Suk-fun , Ip Mary Sau-man , Lam Tai-hing
Published: November 2020

Background: The coronavirus disease 2019 (COVID-19) pandemic has disrupted university teaching globally. The mental health impacts on international university students have been overlooked.

Aims: This study examined the differences in COVID-19-related stressors and mental health impacts between international university students studying in the UK or USA who returned to their home country or region (returnees) and those who stayed in their institution country (stayers), and identified COVID-19-related stressors and coping strategies that were predictors of mental health.

Method: An online questionnaire survey was conducted from April 28 through May 12, 2020 using an exponential, non-discriminative snowball sampling strategy (registered at the National Institutes of Health: NCT04365361).

Results: A total of 124 full-time international university students (36.3% male) were included: 75.8% had returned to their home country or region for reasons related to COVID-19; 77.4% were pursuing a bachelor's program, and 53.2% were in programs with practicum component. 84.7% of all students had moderate-to-high perceived stress, 12.1% had moderate-to-severe symptoms of anxiety and depression, and 17.7% had moderate-to-severe symptoms of insomnia. Compared with returnees, stayers had significantly higher stress from COVID-19-related stressors such as personal health and lack of social support (Cohen's d: 0.57–1.11), higher perceived stress [10-item Perceived Stress Scale (PSS-10)] {22.6 ± 6.2 vs. 19.1 ± 6.1, β [95% confidence interval (CI)]: 4.039 (0.816, 7.261), Cohen's d: 0.52}, and more severe insomnia symptoms [Insomnia Severity Index (ISI)] [11.8 ± 6.1 vs. 7.6 ± 5.2, β (95% CI): 3.087 (0.262, 5.912), Cohen's d: 0.45], with moderate-to-large effect sizes. Compared with males, females reported significantly higher stress from uncertainties about academic program (Cohen's d: 0.45) with a small effect size. In the total sample, stress related to academics (e.g., personal attainment, uncertainties about academic program, and changes in teaching/learning format), health (including personal health and health of family and friends), availability of reliable COVID-19-related information, and lack of social support predicted more negative mental health impacts. Resilience, positive thinking, and exercise were predictors of less severe mental health impacts.

Conclusions: Stayers experienced more adverse mental health impacts than returnees. We call on educators and mental health professionals to provide appropriate support for international students, particularly the stayers, during the pandemic.
The Effect of Physical Exercise Training on Neck and Shoulder Muscle Function Among Military Helicopter Pilots and Crew: A Secondary Analysis of a Randomized Controlled Trial
Authors: Murray Mike , Lange Britt , Søgaard Karen , Sjøgaard Gisela
Published: November 2020

Introduction: This study presents secondary outcome analyses, in terms of muscle function [i.e., maximal voluntary contraction (MVC) and rate of torque development (RTD)] from a parallel group, single blinded, randomized controlled trial introducing a physical exercise training intervention aiming to reduce neck pain among military helicopter pilots and crew-members.

Methods: Participants (50 pilots, 58 crew-members) were recruited from the Royal Danish Air Force and randomized to either an exercise-training-group (ETG; n = 35) or a reference-group (REF; n = 34). Participants in ETG received 20 weeks of self-administered exercise training specifically tailored to target the neck and shoulder muscles. REF received no training. Outcome: (1) MVC was measured for cervical extension and flexion as well as shoulder elevation and abduction, (2) RTD was measured for cervical extension and flexion. Adherence to training was self-reported and categorized as regular if performed at least once a week.

Results: MVC for cervical extension was significantly increased at follow-up in ETG (37.5 ± 11.2 Nm at baseline, change: 2.1 ± 8.3 Nm) compared to REF (38.1 ± 10.7 Nm at baseline, change: −2.4 ± 6.8 Nm) according to intension-to-treat analysis (p = 0.018). Likewise, RTD was significantly increased in ETG for cervical extension (149.6 ± 63.3 Nm/s at baseline, change: 14.7 ± 49.0 Nm/s) compared to REF (165.4 ± 84.7 Nm/s at baseline, change: −16.9±70.9 Nm/s) (p = 0.034). The cervical extension/flexion MVC-ratio was significantly different at follow-up (p = 0.039) between ETG (1.5 ± 0.5 at baseline, change: −0.0 ± 0.3) compared to REF (1.5 ± 0.5 at baseline, change: −0.2 ± 0.4). Per-protocol analysis of MVC, including only participants in ETG with regular training adherence (n = 10), showed a significant increase for cervical extension (33.2 ± 7.3 Nm at baseline, change: 6.0 ± 5.4 Nm) and shoulder elevation right side (143.0 ± 25.8 Nm at baseline, change: 15.8 ± 18.1 Nm).

Conclusion: Physical exercise training significantly improved MVC and RTD in the upper neck extensors. Only approximately 1/3 of participants in ETG adhered to training regularly, which likely attenuated the effectiveness of the training intervention on neck and shoulder muscle function. Future studies should focus on the practical implementation of self-administered exercise training to improve adherence.
COVID-19 Infection Process in Italy and Spain: Are Data Talking? Evidence From ARMA and Vector Autoregression Models
Authors: Monllor Paloma , Su Zhenyu , Gabrielli Laura , Taltavull de La Paz Paloma
Published: November 2020

COVID-19 (coronavirus disease 2019) has spread successfully worldwide in a matter of weeks. After the example of China, all the affected countries are taking hard-confinement measures to control the infection and to gain some time to reduce the significant amount of cases that arrive at the hospital. Although the measures in China reduced the percentages of new cases, this is not seen in other countries that have taken similar measures, such as Italy and Spain. After the first weeks, the worry was whether or not the healthcare system would collapse rather than its response to the patient's needs who are infected and require hospitalization. Using China as a mirror of what could happen in our countries and with the data available, we calculated a model that forecasts the peak of the curve of infection, hospitalization, and ICU bed numbers. We aimed to review the patterns of spread of the virus in the two countries and their regions, looking for similarities that reflect the existence of a typical path in this expansive virulence and the effects of the intervention of the authorities with drastic isolation measures, to contain the outbreak. A model based on Autorregressive and moving average models (ARMA) methodology and including Chinese disease pattern as a proxy, predicts the contagious pattern robustly. Based on the prediction, the hospitalization and intensive care unit (ICU) requirements were also calculated. Results suggest a reduction in the speed of contagion during April in both countries, earlier in Spain than in Italy. The forecast advanced a significant increase in the ICU needs for Spain surpassing 8,000 units by the end of April, but for Italy, ICU needs would decrease in the same period, according to the model. We present the following predictions to inform political leaders because they have the responsibility to maintain the national health systems away from collapsing. We are confident these data could help them into decision-taking and place the capitals (from hospital beds to human resources) into the right place.
Practical Aspects of Model-Based Collision Detection
Authors: Mamedov Shamil , Mikhel Stanislav
Published: November 2020

Recently, with the increased number of robots entering numerous manufacturing fields, a considerable wealth of literature has appeared on the theme of physical human-robot interaction using data from proprioceptive sensors (motor or/and load side encoders). Most of the studies have then the accurate dynamic model of a robot for granted. In practice, however, model identification and observer design proceeds collision detection. To the best of our knowledge, no previous study has systematically investigated each aspect underlying physical human-robot interaction and the relationship between those aspects. In this paper, we bridge this gap by first reviewing the literature on model identification, disturbance estimation and collision detection, and discussing the relationship between the three, then by examining the practical sides of model-based collision detection on a case study conducted on UR10e. We show that the model identification step is critical for accurate collision detection, while the choice of the observer should be mostly based on computation time and the simplicity and flexibility of tuning. It is hoped that this study can serve as a roadmap to equip industrial robots with basic physical human-robot interaction capabilities.
Traditional Products and New Developments in the Restaurant Sector in East Africa. The Case Study of Nakuru County, Kenya
Authors: Zocchi Dauro M. , Fontefrancesco Michele F.
Published: November 2020

Over the last 20 years, we have witnessed worldwide a renewed interest in local food products and traditional cuisine. Addressing this demand, the catering industry has played a pivotal role in reviving local food heritage and traditions. While several studies have explored the evolution of this trend in Europe, little attention has been given to this phenomenon in contemporary Africa. To partially fill this gap in the literature, we conducted an ethnographic study to investigate the role of the catering sector in recovering and promoting food and gastronomic heritage in Nakuru County, an emerging Kenyan agricultural and tourist hub. Specifically, we aimed at understanding the main drivers behind the offering and demand for traditional ingredients and recipes. Fieldwork was conducted through the analysis of 41 restaurants and hotels, and data collection was completed through semi-structured interviews with 51 professionals, including owners, food and beverage managers, and chefs. We reported 33 recipes and ingredients tied to Kenyan culinary traditions. Some differences in the role of Kenyan cuisine emerged, with the differentiation mostly linked to the customer profiles. In particular, attention toward traditional foods was more accentuated in restaurants aimed at middle- and high-income Kenyan customers and for specific products namely African leafy vegetables and indigenous chicken, locally known askuku kienyeji. Concurrently, we discovered that the inclusion of these products on the restaurant menus implied an incipient localization of the food supply chains based on self-production or direct commercial relationships with small-scale producers. The research highlighted how the relaunch of traditional food and cuisine develops from a demand for healthy and natural products rather than a search for cultural authenticity. Based on the specificities of the local market, this fosters the creation of alternative supply strategies to cope with the poor quality of ingredients, price fluctuations, and discontinuity of the supply. In this sense, the research suggests also considering tangible factors linked to the technological and logistical conditions of the trade and safety of food to understand the drivers behind the rediscovery of local and traditional foods.
Long-Lasting Impact of Maternal Immune Activation and Interaction With a Second Immune Challenge on Pig Behavior
Authors: Rymut Haley E. , Bolt Courtni R. , Caputo Megan P. , Houser Alexandra K. , Antonson Adrienne M. , Zimmerman Jalisa D. , Villamil Maria B. , Southey Bruce R. , Rund Laurie A. , Johnson Rodney W. , Rodriguez-Zas Sandra L.
Published: November 2020

The combined effects on pig behavior of maternal immune challenge during gestation followed by a second immune challenge later in life have not been studied. Porcine reproductive and respiratory syndrome virus (PRRSV) infection during gestation can elicit maternal immune activation (MIA) yet the interactions with the offspring response to a second immune challenge after birth remains unexplored. Knowledge on the response to viral challenges in rodents has been gained through the use of the viral mimetic polyinosinic-polycytidylic acid (Poly(I:C)), yet the effects of this immune stimulant on pig behavior have not been assessed. This study advances the understanding of the combined effect of MIA and a second immune challenge later in life on female and male pig behavior. Three complementary experiments enabled the development of an effective Poly(I:C) challenge in pigs, and testing the interaction between PRRSV-elicited MIA, Poly(I:C) challenge at 60 days of age, and sex on behaviors. Individual-level observations on sickness, locomotor, and social behaviors were measured 1–3 h after Poly(I:C) challenge. Vomiting, panting, lethargy, walking, laying, playing, and touching behaviors were analyzed using generalized linear mixed effect models. Results indicated that a Poly(I:C) dose of 1 mg/kg within 1 h after injection increased the incidence of laying and sickness behavior. The Poly(I:C) challenge decreased the incidence of locomotor behaviors and activity levels. Pigs exposed to MIA had lower rates of social behaviors such as playing. The combined effect of PRRSV-elicited MIA and Poly(I:C) immune challenge further sensitized the pigs to behavior disruption across sexes including changes in sternal and lateral laying, walking, lethargy, and touching incidence. Notably, the effects of Poly(I:C) immune challenge alone on behaviors tended to be more extreme in males, whereas the effects of Poly(I:C) following MIA tended to be more extreme in females. Our findings demonstrate that MIA and Poly(I:C) affected behaviors, and the viral mimetic effects shortly after injection can offer insights into the prolonged effect of postnatal viral infections on feeding, social interactions and health status. Management practices that reduce the likelihood of gestational diseases and accommodate for behavioral disruptions in the offspring can minimize the impact of MIA.
Genetic Analysis of Cachavirus-Related Parvoviruses Detected in Pet Cats: The First Report From China
Authors: Ji Jun , Hu Wen , Liu Qiang , Zuo Kejing , Zhi Guanglin , Xu Xin , Kan Yunchao , Yao Lunguang , Xie Qingmei
Published: November 2020

In this study, members of the Carnivore chaphamaparvovirus species 1, closely related to a virus previously reported in dog feces named cachavirus was identified for the first time in feces of Chinese cats. Screening tests using rectal swabs from 171 diarrheic and 378 healthy cats collected from Henan, Anhui, and Zhejiang provinces in China revealed two samples from diarrheic cats that were positive for cachavirus, but statistical analysis indicated no association between the presence of the virus and clinical signs (p > 0.05). Subsequently, two partial genome sequences [from nucleotides 479–4123, according to the strains from dogs (cachavirus)] of the two strains from cats (cachavirus-cat1 and -cat2) were amplified. The NS1 and VP1 sites of cachavirus-cat1 and -cat2 shared a high identity of 91.9 and 97.0% with reported cachaviruses, respectively, but lower identity of 74.8 and 73.2% with another carnivore chaphamaparvovirus named fechaviruses detected in cats, respectively, indicated the two strains might origin from dogs. These findings improve our understanding of the diversity and tropism of viruses in Carnivore chaphamaparvovirus species 1 which now include both dogs and now cats viruses.
Combining Optimization and Simulation for Designing a Robust Short-Sea Feeder Network
Authors: Medbøen Carl Axel Benjamin , Holm Magnus Bolstad , Msakni Mohamed Kais , Fagerholt Kjetil , Schütz Peter
Published: November 2020

Here we study a short-sea feeder network design problem based on mother and daughter vessels. The main feature of the studied system is performing transshipment of cargo between mother and daughter vessels at appropriate locations at sea. This operation requires synchronization between both types of vessels as they have to meet at the same location at the same time. This paper studies the problem of designing a synchronized feeder network, explicitly accounting for the effect of uncertain travel times caused by harsh weather conditions. We propose an optimization-simulation framework to find robust solutions for the transportation system. The optimization model finds optimal routes that are then evaluated by a discrete-even simulation model to measure their robustness under uncertain weather conditions. This process of optimization simulation is repeated until a satisfactory condition is reached. To find even better solutions, we include different performance-improving strategies by adding robustness during route generation or exploiting flexibility in sailing speed to recover from delays. We apply the solution method to a case based on realistic data from a Norwegian shipping company. The results show that the method finds near-optimal solutions that offer robustness against schedule perturbations due to harsh weather. They also highlight the importance of considering uncertainty when designing a short-sea feeder network with transshipment at sea.
Justice for the Crowd: Organizational Justice and Turnover in Crowd-Based Labor
Authors: Song Xiaochuan , Lowman Graham H. , Harms Peter
Published: November 2020

Crowd-based labor has been widely implemented to solve human resource shortages cost-effectively and creatively. However, while investigations into the benefits of crowd-based labor for organizations exist, our understanding of how crowd-based labor practices influence crowd-based worker justice perceptions and worker turnover is notably underdeveloped. To address this issue, we review the extant literature concerning crowd-based labor platforms and propose a conceptual model detailing the relationship between justice perceptions and turnover within the crowd-based work context. Furthermore, we identify antecedents and moderators of justice perceptions that are specific to the crowd-based work context, as well as identify two forms of crowd-based turnover as a result of justice violations: requester and platform turnover. In doing so, we provide a novel conceptual model for advancing nascent research on crowd-based worker perceptions and turnover.
Aerobic Rice with or without Strategic Irrigation in the Subtropics
Authors: Silwal Sachesh , Bhattarai Surya P. , Midmore David J.
Published: November 2020

Modern rice varieties adapted to aerobic (dryland) conditions have expanded to new rice growing systems thanks to their plasticity in adapting to rainfed and irrigated conditions. This is important because, as water becomes scarce in paddy rice regions (as it is already in Australia), there will be a move towards tropical to subtropical dryland rainfed rice with attendant problems of drought and low temperature. To assess rice adaptability in the wet season of the semi-arid subtropical conditions of coastal central Queensland, field experiments were established for a late season (in January) planting in 2014 and early season planting in November 2015 with 13 varieties developed by Australian Agriculture Technologies (AAT) Ltd were seeded in a vertisol soil. This was to assess their adaptation to rainfed conditions and their response to strategic irrigation. Water scarcity and low temperature prior to and at flowering were important factors constraining yield. Early flowering varieties in the late season planting escaped the otherwise cold and drought stress during the reproductive stage and had higher yields. In the second year, earlier planting made possible with strategic irrigation avoided the low temperature constraint on yield, but without follow-up strategic irrigation, yields were still low. The average yield of varieties increased from 1.5 times (AAT 4) to 16.3 times (AAT 15) with strategic irrigation compared with rainfed yields averaged across years. The increase in yield with strategic irrigation was associated with a greater leaf area index, spikelet fertility, and instantaneous water use efficiency during flowering. Strategic irrigation concentrated roots in the top 15 cm, but differences in yield between varieties under rainfed conditions were not related to root properties. It is important to consider variations in flowering time, yield potential, and drought patterns when developing rice varieties for rainfed semi-arid tropical conditions, as well as when quantifying the benefits of strategic irrigation.
Studying Gene Expression in Irradiated Barley Cultivars: PM19L-like and CML31-like Expression as Possible Determinants of Radiation Hormesis Effect
Authors: Gorbatova Irina V. , Kazakova Elizaveta A. , Podlutskii Mikhail S. , Pishenin Ivan A. , Bondarenko Vladimir S. , Dontsova Aleksandra A. , Dontsov Dmitriy P. , Snegirev Aleksei S. , Makarenko Ekaterina S. , Bitarishvili Sofia V. , Lychenkova Maria A. , Chizh Taras V. , Volkova Polina Yu.
Published: November 2020

Gamma (γ)-irradiation of plants at low doses can provoke a broad range of growth-stimulating effects. In order to reveal universal target genes that are involved in molecular pathways of radiation hormesis establishment, we studied nine barley cultivars for their tolerance to γ-irradiation of seeds. Four morphological traits were assessed in barley seedlings after γ-irradiation of seeds at 20 Gy. Nine cultivars were sorted according to the sensitivity to irradiation as γ-stimulated, “no morphological effect”, or γ-inhibited. Gene expression of 17 candidate genes was evaluated for the 7 most contrasting cultivars. Changes in expression of barley homologues of PM19L and CML31 were suggested as possible determinants of radiation hormesis effect. The possible role of jasmonate signaling in roots in radiation growth stimulations was revealed. Morphological analysis and gene expression study showed that the genetic background of a cultivar plays an important role in eustress responses to low-dose γ-irradiation of seeds.
Transport, Associated Handling Procedures and Behaviour of Calves Marketed through Chilean Auction Markets
Authors: Bravo Viviana M. , Knowles Toby G. , Gallo Carmen
Published: November 2020

In Chile, selling animals through livestock markets is common. At markets, stressful events like loading, unloading and travel are at least duplicated. We described procedures associated with transport of calves at 20 markets and evaluated compliance with Chilean law by performing a survey of drivers who transport calves from origin farms to markets (OM) and from markets to destination (MD). During loading and unloading, we evaluated handling by stockpersons, facilities, fitness for transport, and behavioural indicators of the calves through direct observation using protocols. A total of 80% of drivers claimed having the training required by law. The mean travel time was 1 h 31 min for OM and 1 h 44 min for MD journeys (overall range 5 min–40.5 h). Most drivers used bedding material and provided adequate space availability. A total of 99.2% of the observed calves were assessed as fit to transport; slipping, turning back, vocalizing and balking were frequent behaviours observed during loading and unloading. Prohibited practices like prodding and hitting using driving devices were still observed, mainly during loading. Compliance with the law during transport of calves was adhered to; however, the associated handling within markets was still inadequate, evidencing need for training in order to improve animal welfare.
Effects of Different Laying Hen Species on Odour Emissions
Authors: Lu Dongdong , Mi Jiandui , Wu Yinbao , Liang Juanboo , Liao Xindi , Wang Yan
Published: November 2020

Odour is one of the main environmental concerns in the laying hen industry and may also influence animal health and production performance. Previous studies showed that odours from the laying hen body are primarily produced from the microbial fermentation (breakdown) of organic materials in the caecum, and different laying hen species may have different odour production potentials. This study was conducted to evaluate the emissions of two primary odorous gases, ammonia (NH3) and hydrogen sulphide (H2S), from six different laying hen species (Hyline, Lohmann, Nongda, Jingfen, Xinghua and Zhusi). An in vitro fermentation technique was adopted in this study, which has been reported to be an appropriate method for simulating gas production from the microbial fermentation of organic materials in the caecum. The results of this study show that Jingfen produced the greatest volume of gas after 12 h of fermentation (p < 0.05). Hyline had the highest, while Lohmann had the lowest, total NH3 emissions (p < 0.05). The total H2S emissions of Zhusi and Hyline were higher than those of Lohmann, Jingfen and Xinghua (p < 0.05), while Xinghua exhibited the lowest total H2S emissions (p < 0.05). Of the six laying hen species, Xinghua was identified as the best species because it produced the lowest total amount of NH3 + H2S (39.94 µg). The results for the biochemical indicators showed that the concentration of volatile fatty acids (VFAs) from Zhusi was higher than that for the other five species, while the pH in Zhusi was lower (p < 0.01), and the concentrations of ammonium nitrogen (NH4+), uric acid and urea in Xinghua were lower than those in the other species (p < 0.01). Hyline had the highest change in SO42− concentration during the fermentation processes (p < 0.05). In addition, the results of the correlation analysis suggested that NH3 emission is positively related to urease activities but is not significantly related to the ureC gene number. Furthermore, H2S emission was observed to be significantly related to the reduction of SO42− but showed no connection with the aprA gene number. Overall, our findings provide a reference for future feeding programmes attempting to reduce odour pollution in the laying hen industry.
Dietary ARA Improves COX Activity in Broodstock and Offspring Survival Fitness of a Model Organism (Medaka Oryzias latipes)
Authors: Kowalska Agata , Kamaszewski Maciej , Czarnowska-Kujawska Marta , Podlasz Piotr , Kowalski Radosław K.
Published: November 2020

A 3-week feeding trial was conducted in medaka broodstock (age five months) to examine the effect of dietary arachidonic acid (ARA) level (range: 4–23 mg g−1 of total fatty acids (TFAs)) on fertility, cyclooxygenase (COX) activity, egg size, sperm motility parameters, hatching rate and weight of hatch, survival and growth of larvae. After spawn induction and dietary exposure to 4 mg ARA g−1 TFA, broodstock were fed a diet containing ARA in the amounts: 4 (continued, as control), 5, 14 and 23 mg g−1 TFA. COX1 activity in the liver and the number of COX2-positive cells in the ovaries was increased in females fed the diets containing the two highest amounts of ARA. The highest sperm motility parameters were observed in males fed a diet containing 23 mg ARA g−1 TFA. The hatchability rate and bodyweight of hatchlings were higher in the group obtained from broodstock fed a diet containing 23 mg ARA g−1 TFA (79% and 0.66 mg fish−1, respectively) compared with 4 mg ARA g−1 TFA (50% and 0.40 mg fish−1). The average mortality of offspring obtained from this group at 7 days post hatching was significantly higher than that of all other groups.