bluuebunny/crossref_metadata_2025 huggingface dataset:
Scaling of classification systems—effects of class precision on detection accuracy from medium resolution multispectral data
Authors: Gann Daniel , Richards Jennifer
Published: December 2022

Context

Land-cover class definitions are scale-dependent. Up-scaling categorical data must account for that dependence, but most decision rules aggregating categorical data do not produce scale-specific class definitions. However, non-hierarchical, empirically derived classification systems common in phytosociology define scale-specific classes using species co-occurrence patterns.

Objectives

Evaluate tradeoffs in class precision and representativeness when up-scaling categorical data across natural landscapes using the multi-dimensional grid-point (MDGP)-scaling algorithm, which generates scale-specific class definitions; and compare spectral detection accuracy of MDGP-scaled classes to ‘majority-rule’ aggregated classes.

Methods

Vegetation maps created from 2-m resolution WorldView-2 data for two Everglades wetland areas were scaled to the 30-m Landsat grid with the MDGP-scaling algorithm. A full-factorial analysis evaluated the effects of scaled class-label precision and class representativeness on compositional information loss and detection accuracy of scaled classes from multispectral Landsat data.

Results

MDGP‐scaling retained between 3.8 and 27.9% more compositional information than the majority rule as class-label precision increased. Increasing class-label precision and information retention also increased spectral class detection accuracy from Landsat data between 1 and 8.6%. Rare class removal and increase in class-label similarity were controlled by the class representativeness threshold, leading to higher detection accuracy than the majority rule as class representativeness increased.

Conclusions

When up-scaling categorical data across natural landscapes, negotiating trade-offs in thematic precision, landscape-scale class representativeness and increased information retention in the scaled map results in greater class-detection accuracy from lower-resolution, multispectral, remotely sensed data. MDGP-scaling provides a framework to weigh tradeoffs and to make informed decisions on parameter selection.
Deterioration of visual quality and acuity as the first sign of ceroid lipofuscinosis type 3 (CLN3), a rare neurometabolic disease
Authors: Purzycka-Olewiecka Joanna Karolina , Hetmańczyk-Sawicka Katarzyna , Kmieć Tomasz , Szczęśniak Dominika , Trubicka Joanna , Krawczyński Maciej , Pronicki Maciej , Ługowska Agnieszka
Published: December 2022

Ceroid lipofuscinosis type 3 (CLN3) is an autosomal recessive, neurodegenerative metabolic disease. Typical clinical symptoms include progressive visual loss, epilepsy of unknown etiology and dementia. Presence of lipofuscin deposits with typical pattern of ‘fingerprints’ and vacuolized lymphocytes suggest the diagnosis of CLN3. Cause of CLN3 are mutations in the CLN3 gene, among which the most frequently found is the large deletion 1.02 kb spreading on exons 7 and 8. We present 4 patients from 2 families, in whom the deterioration of visual quality and acuity was observed as first clinical sign, when they were a few years old and it was successively accompanied by symptoms of neurologic deterioration (like generalized convulsions with consciousness impairment). In all patients the 1.02 kb deletion in the CLN3 gene was detected in homo- or heterozygosity with other CLN3 pathogenic variant. Ultrastructural studies revealed abnormal structures corresponding to ‘fingerprint’ profiles (FPPs) in conjunctival endothelial cells. It should be emphasized that in patients with blindness of unknown cause the diagnosis of ceroid lipofuscinosis should be considered and in older children—especially CLN3. The facility of the analysis for the presence of 1.02 kb deletion and economic costs are a solid argument for intensive use of this test in the diagnostic procedure of CLN3.
FDG–PET findings associated with various medical procedures and treatments
Authors: Okuyama Chio , Higashi Tatsuya , Ishizu Koichi , Saga Tsuneo
Published: December 2022

[18F]-fluorodeoxyglucose (FDG) positron emission tomography (PET) is a well-established modality with high sensitivity for the diagnosis and staging of oncologic patients. FDG is taken up by the glucose transporter of the cell membrane and becomes trapped within the cell. In addition to malignant neoplasms, active inflammatory lesions and some kinds of benign tumors also accumulate FDG. Moreover, the degree of uptake into normal organs and tissues depends on various physiological conditions, which is affected by various medical procedures, treatments, and drugs. To avoid misleading interpretations, it is important to recognize possible situations of unexpected abnormal accumulation that mimic tumor lesions. In this review, we present various FDG findings associated with surgical or medical procedures and treatments. Some findings reflect the expected physiological reaction to treatment, and some show inflammation due to prior procedures. Occasionally, FDG–PET visualizes other disorders that are unrelated to the malignancy, which may be associated with the adverse effects of certain drugs that the patient is taking. Careful review of medical records and detailed interviews of patients are thus necessary.
Combined time-restricted feeding and cisplatin enhance the anti-tumor effects in cisplatin-resistant and -sensitive lung cancer cells
Authors: Li Jianling , Chen Qianyao , Shi Dan , Lian Xuemei
Published: December 2022

Combination therapy as an important treatment option for lung cancer has been attracting attention due to the primary and acquired resistance of chemotherapeutic drugs in the clinical application. In the present study, as a new therapy strategy, concomitant treatment with time-restricted feeding (TRF) plus cisplatin (DDP) on lung cancer growth was investigated in DDP-resistant and DDP-sensitive lung cancer cells. We first found that TRF significantly enhanced the drug susceptibility of DDP in DDP-resistant A549 (A549/DDP) cell line, illustrated by reversing the inhibitory concentration 50 (IC50) values of A549/DDP cells to normal level of parental A549 cells. We also found that TRF markedly enhanced DDP inhibition on cell proliferation, migration, as well as promoted apoptosis compared to the DDP alone group in A549, H460 and A549/DDP cells lines. We further revealed that the synergistic anti-tumor effect of combined DDP and TRF was greater than that of combined DDP and simulated fasting condition (STS), a known anti-tumor cellular medium. Moreover, mRNA sequence analysis from A549/DDP cell line demonstrated the synergistic anti-tumor effect involved in upregulated pathways in p53 signaling pathway and apoptosis. Notably, compared with the DDP alone group, combination of TRF and DDP robustly upregulated the P53 protein expression without mRNA level change by regulating its stability via promoting protein synthesis and inhibiting degradation, revealed by cycloheximide and MG132 experiments. Collectively, our results suggested that TRF in combination with cisplatin might be an additional novel therapeutic strategy for patients with lung cancer.
Mindshaping and Non-Gricean Approaches to Language Evolution
Authors: Vierkant Tillmann
Published: December 2022

Orthodoxy has it that language evolution requires Gricean communicative intentions and therefore an understanding of nested metarepresentations. The problem with this orthodoxy is that it is hard to see how non-linguistic creatures could have such a sophisticated understanding of mentality. Some philosophers like Bar-On (The Journal of Philosophy 110 (6): 293-330, 2013a; Mind and Language 28 (3): 342-375, 2013b) have attempted to develop a non-Gricean account of language acquisition building on the information-rich and subtle communicative powers of expressive behaviours. This paper argues that this approach shares a number of instructive similarities with the literature on the cognitive conception of language. It shows how expressive accounts (and others) can learn from insights in that tradition and identifies a problem with the role of intentional actions in expressive accounts that can be solved by understanding expressive behaviours more as cognitive rather than communicative tools.
Sexual Risk Behavior, Sexism, and Prejudices Towards Sexual Openness, Homosexuality, and Trans Individuals Among Young People in Spain and the UK
Authors: Alonso-Martínez Laura , Forrest Simon , Heras-Sevilla Davinia , Hönekopp Johannes , Fernández-Hawrylak María
Published: December 2022

Introduction

Sexism, lack of sexual freedom, and negative attitudes towards minorities are related to risky and discriminatory sexual behaviors. To date, no cross-cultural comparisons have been made regarding these sexual behaviors and attitudes. The study also explores the sexuality competencies of young adults undertaking vocational courses in higher education before their transition to professional practice.

Methods

The sample comprised 1235 university students (670 Spanish and 565 British) aged between 18 and 52 years (M = 22.06, SD = 4.38). During the years 2020 and 2021, respondents completed a survey structured in six standardized scales (HPSC, DSS, SOS, HATH, EANT, and TIBS).

Results

Results report that British students show less gender adherence (DSS) while the Spanish ones show more positive attitudes towards trans individuals (EANT) and pleasure (SOS). These findings indicate that students in this research engage in more sexual risk behaviors (HPSC), display similar rigid gender adherences (DSS), and more positive erotophilic (SOS), homophilic (HATH), and trans-friendly attitudes (EANT and TIBS) than those in previous studies. There were, however, significant differences by gender, religion, and program of study.

Conclusions

The research demonstrates the relevance of measuring cultural factors related to sexual behaviors and attitudes in youth and reflects a lack of attention to these issues in educational and healthcare settings. This is despite its broad impact on people’s health, as young people are more likely to display increased risky sexual behaviors.

Policy Implications

These findings emphasize the importance of the development of updated strategies in sex education among young people. The added importance of doing so with students in higher education who are likely to enter professions where they will educate or influence others on these matters is identified.
The influencing mechanism of O2, H2O, and CO2 on the H2S removal of food waste digestate-derived biochar with abundant minerals
Authors: Huang Dandan , Wang Ning , Bai Xinyue , Chen Yuke , Xu Qiyong
Published: December 2022

Hydrogen sulfide (H2S) removal has been a significant concern in various industries. In this study, food waste digestate-derived biochar (DFW-BC), a by-product of food waste treatment with abundant minerals, was assessed for removing H2S from different simulated biogas containing oxygen (O2) and carbon dioxide (CO2) and under different moisture (H2O) contents (0% and 20%) of biochar. The influencing mechanisms of the gas conditions combined with the moisture contents were also investigated. The results showed an H2S removal of 1.75 mg g−1 for dry biochar under pure H2S, 4.29 mg g−1 for dry biochar under H2S + O2, 5.29 mg g−1 for humid biochar under H2S, and 12.50 mg g−1 for humid biochar under H2S + O2. For dry DFW-BC, the high Fe content was responsible for the O2 enhancement. In contrast, O2 + H2O activated the catalytic H2S oxidation of the less reactive minerals (mainly Ca). The inhibition of CO2 on H2S adsorption was not obvious for dry DFW-BC; the specific pore structure may have provided a buffer against the physisorption competition of CO2. However, when H2O was present on DFW-BC, the changes in critical biochar properties and sulfur speciation as opposed to that without H2O implied an evident occurrence of CO2 chemisorption. This CO2 chemisorption partially hindered O2 + H2O enhancement, decreasing the H2S removal capacity from 12.50 to 8.88 mg g−1. The negative effect was ascribed to mineral carbonation of CO2, neutralizing the alkaline surface and immobilizing metal oxides, which thus reduced the acceleration in H2S dissociation and activation in catalytic H2S oxidation by O2 + H2O.

Graphical Abstract
“Smart Knee Implants: An Overview of Current Technologies and Future Possibilities”
Authors: Kelmers Edgars , Szuba Agata , King Samuel W. , Palan Jeya , Freear Steven , Pandit Hemant G. , van Duren Bernard H.
Published: December 2022

Background

This article focuses on clinical implementation of smart knee implants for total knee replacement and the future development of smart implant technology. With the number of total knee replacements undertaken growing worldwide, smart implants incorporating embedded sensor technology offer opportunity to improve post-operative recovery, reducing implant failure rates, and increasing overall patient satisfaction.

Methods

A literature review on smart implants, historical prototypes, current clinically available smart implants, and the future potential for conventional implant instrumentation with embedded sensors and electronics was undertaken.

Results

The overview of current and future technology describes use cases for various diagnostic and therapeutic treatment solutions.

Conclusion

Smart knee implants are at an early development stage, with the first generation of smart implants being available to patients and with more novel technologies under development.
H3-T6SS of Pseudomonas aeruginosa PA14 contributes to environmental adaptation via secretion of a biofilm-promoting effector
Authors: Yang Yantao , Pan Damin , Tang Yanan , Li Jiali , Zhu Kaixiang , Yu Zonglan , Zhu Lingfang , Wang Yao , Chen Peng , Li Changfu
Published: December 2022

Microbial species often occur in complex communities and exhibit intricate synergistic and antagonistic interactions. To avoid predation and compete for favorable niches, bacteria have evolved specialized protein secretion systems. The type VI secretion system (T6SS) is a versatile secretion system widely distributed among Gram-negative bacteria that translocates effectors into target cells or the extracellular milieu via various physiological processes. Pseudomonas aeruginosa is an opportunistic pathogen responsible for many diseases, and it has three independent T6SSs (H1-, H2-, and H3-T6SS). In this study, we found that the H3-T6SS of highly virulent P. aeruginosa PA14 is negatively regulated by OxyR and OmpR, which are global regulatory proteins of bacterial oxidative and acid stress. In addition, we identified a H3-T6SS effector PA14_33970, which is located upstream of VgrG3. PA14_33970 interacted directly with VgrG3 and translocated into host cells. Moreover, we found that H3-T6SS and PA14_33970 play crucial roles in oxidative, acid, and osmotic stress resistance, as well as in motility and biofilm formation. PA14_33970 was identified as a new T6SS effector promoting biofilm formation and thus named TepB. Furthermore, we found that TepB contributes to the virulence of P. aeruginosa PA14 toward Caenorhabditis elegans. Overall, our study indicates that H3-T6SS and its biofilm-promoting effector TepB are regulated by OxyR and OmpR, both of which are important for adaptation of P. aeruginosa PA14 to multiple stressors, providing insights into the regulatory mechanisms and roles of T6SSs in P. aeruginosa.
China’s emergence and development challenges that China faces in Central Asia
Authors: Zhang Chi
Published: December 2022

Development in Central Asia faces intensifying headwinds in various aspects. Terrorism and political instability have been the primary sources of concern for this chessboard of rivaling great powers. The US’s withdrawal from Afghanistan left a power vacuum, and the region’s future is further clouded by elevated uncertainty. The so-called ‘new Cold War’ discourse is becoming a self-fulfilling prophecy that contextualizes regional geopolitical maneuverings. These developments present a pressing need to evaluate development challenges in Central Asia in the context of China’s rising influence in the region through the Belt and Road Initiative and other regional frameworks, such as the Shanghai Cooperation Organization and China Pakistan Economic Corridor.

This paper seeks to examine the shifting geopolitical and geoeconomic landscape in Central Asia in the context of global ideological confrontations and the regional Great Game between China and Russia. Drawing on think tank reports, English-language media reports, and scholarly works, it argues that China’s investment and development strategy in Central Asia can be improved by giving geopolitical and geoeconomic factors full consideration. The changing political dynamics in the region have significant implications for China’s engagement with Central Asian countries, its broader Belt and Road Initiative extending through Central Asia to Europe, and development challenges that transcend the dualistic categorization of development and security.
Urban-rural differences in the pregnancy-related adverse outcome
Authors: Mehrnoush Vahid , Ranjbar Amene , Banihashemi Farzaneh , Darsareh Fatemeh , Shekari Mitra , Shirzadfardjahromi Malihe
Published: December 2022

Background

Little is known about potential urban-rural differences in adverse pregnancy outcomes. The purpose of this study is to look into the urban-rural differences in the trend of adverse maternal and neonatal outcomes.

Methods

We retrospectively assessed the pregnancy outcome of singleton pregnant mothers who gave birth at a tertiary hospital in Bandar Abbas, Iran, between January 1st, 2020, and January 1st, 2022. Mothers were divided into two groups based on living residency: 1) urban groupand 2) rural group.Demographic factors, obstetrical factors, maternal comorbidities, and adverse maternal and neonatal outcomeswere extracted from the electronic data of each mother. The Chi-square testwas used to compare differences between the groups for categorical variables. Logistic regression models were used to assess the association of adverse pregnancy, childbirth, and neonatal outcome with living residency.

Results

Of 8888 mothers that gave birth during the study period, 2989 (33.6%) lived in rural areas. Adolescent pregnancy was more common in the rural area. Urban mothers had a higher education than rural mothers. Rural mothers were at higher risk for preterm birth aOR 1.81 (CI:1.24-2.99), post-term pregnancy aOR 1.5 (CI: 1.07-2.78), anemia aOR 2.02 (CI:1.07-2.34), low birth weight (LBW) aOR 1.89 (CI: 1.56-2.11), need for neonatal resuscitation aOR 2.66 (CI: 1.78-3.14), and neonatal intensive care unit (NICU) admission aOR 1.98 (CI:1.34-2.79). On the other hand, the risk of cesarean section was significantly lower compared to urban mothers aOR 0.58 (CI: 0.34-0.99).

Conclusions

Our study discovered that mothers living in rural areas had a higher risk of developing anemia, preterm birth, post-term pregnancies, LBW, need for neonatal resuscitation, and NICU admission, but a lower risk of cesarean section.
Multiphysics‐Informed Neural Networks for Coupled Soil Hydrothermal Modeling
Authors: Wang Yanling , Shi Liangsheng , Hu Xiaolong , Song Wenxiang , Wang Lijun
Published: December 2022

Soil water and heat transport are two physical processes that are described by the Richardson–Richards equation and heat transport equation, respectively. Soil water and heat motion directly control transport or indirectly influence parameters. The physics‐informed neural network (PINN) is a new method that combines deep learning and physical laws that approximates and learns physical dynamics better than traditional data‐driven deep learning methods. In this study, we propose multiphysics‐informed neural networks for soil water‐heat systems, in which the soil moisture and temperature information complement each other well. With our framework, existing soil moisture neural networks are improved to reduce their dependency on the soil moisture measurement density. Furthermore, soil moisture data are employed to promote soil temperature dynamic learning and soil thermal conductivity estimation. Moreover, soil temperature data assist in recovering the nonlinearity of the soil hydraulic conductivity through hydrothermal coupling constraints, allowing better estimations of the soil water flux density. The gradient‐based annealing method is applied to adapt the loss function, which satisfactorily balances the water‐heat transport governing equation constraints on the neural networks. The robustness and generalizability of our framework are examined under diverse scenarios. This work demonstrates the mutual compensation of multisource data in coupled physical processes in a deep learning framework and highlights the significance of appropriate multiphysical constraints designed for nonlinear parameter recovery in PINNs.
Neuroimaging profiling identifies distinct brain maturational subtypes of youth with mood and anxiety disorders
Authors: Ge Ruiyang , Sassi Roberto , Yatham Lakshmi N. , Frangou Sophia
Published: December 2022

Mood and anxiety disorders typically begin in adolescence and have overlapping clinical features but marked inter-individual variation in clinical presentation. The use of multimodal neuroimaging data may offer novel insights into the underlying brain mechanisms. We applied Heterogeneity Through Discriminative Analysis (HYDRA) to measures of regional brain morphometry, neurite density, and intracortical myelination to identify subtypes of youth, aged 9–10 years, with mood and anxiety disorders (N = 1931) compared to typically developing youth (N = 2823). We identified three subtypes that were robust to permutation testing and sample composition. Subtype 1 evidenced a pattern of imbalanced cortical-subcortical maturation compared to the typically developing group, with subcortical regions lagging behind prefrontal cortical thinning and myelination and greater cortical surface expansion globally. Subtype 2 displayed a pattern of delayed cortical maturation indicated by higher cortical thickness and lower cortical surface area expansion and myelination compared to the typically developing group. Subtype 3 showed evidence of atypical brain maturation involving globally lower cortical thickness and surface coupled with higher myelination and neural density. Subtype 1 had superior cognitive function in contrast to the other two subtypes that underperformed compared to the typically developing group. Higher levels of parental psychopathology, family conflict, and social adversity were common to all subtypes, with subtype 3 having the highest burden of adverse exposures. These analyses comprehensively characterize pre-adolescent mood and anxiety disorders, the biopsychosocial context in which they arise, and lay the foundation for the examination of the longitudinal evolution of the subtypes identified as the study sample transitions through adolescence.
LPA receptor 1 (LPAR1) is a novel interaction partner of Filamin A that promotes Filamin A phosphorylation, MRTF-A transcriptional activity and oncogene-induced senescence
Authors: Konopa Andreas , Meier Melanie A. , Franz Miriam J. , Bernardinelli Emanuele , Voegele Anna-Lena , Atreya Raja , Ribback Silvia , Roessler Stephanie , Aigner Achim , Singer Kerstin , Singer Stephan , Sarikas Antonio , Muehlich Susanne
Published: December 2022

Myocardin-related transcription factors A and B (MRTFs) are coactivators of Serum Response Factor (SRF), which controls fundamental biological processes such as cell growth, migration, and differentiation. MRTF and SRF transcriptional activity play an important role in hepatocellular carcinoma (HCC) growth, which represents the second leading cause of cancer-related mortality in humans worldwide. We, therefore, searched for druggable targets in HCC that regulate MRTF/SRF transcriptional activity and can be exploited therapeutically for HCC therapy. We identified the G protein-coupled lysophosphatidic acid receptor 1 (LPAR1) as a novel interaction partner of MRTF-A and Filamin A (FLNA) using fluorescence resonance energy transfer-(FRET) and proximity ligation assay (PLA) in vitro in HCC cells and in vivo in organoids. We found that LPAR1 promotes FLNA phosphorylation at S2152 which enhances the complex formation of FLNA and MRTF-A, actin polymerization, and MRTF transcriptional activity. Pharmacological blockade or depletion of LPAR1 prevents FLNA phosphorylation and complex formation with MRTF-A, resulting in reduced MRTF/SRF target gene expression and oncogene-induced senescence. Thus, inhibition of the LPAR1–FLNA–MRTF-A interaction represents a promising strategy for HCC therapy.
Molecular mechanism of substrate recognition by folate transporter SLC19A1
Authors: Dang Yu , Zhou Dong , Du Xiaojuan , Zhao Hongtu , Lee Chia-Hsueh , Yang Jing , Wang Yijie , Qin Changdong , Guo Zhenxi , Zhang Zhe
Published: December 2022

Folate (vitamin B9) is the coenzyme involved in one-carbon transfer biochemical reactions essential for cell survival and proliferation, with its inadequacy causing developmental defects or severe diseases. Notably, mammalian cells lack the ability to de novo synthesize folate but instead rely on its intake from extracellular sources via specific transporters or receptors, among which SLC19A1 is the ubiquitously expressed one in tissues. However, the mechanism of substrate recognition by SLC19A1 remains unclear. Here we report the cryo-EM structures of human SLC19A1 and its complex with 5-methyltetrahydrofolate at 3.5–3.6 Å resolution and elucidate the critical residues for substrate recognition. In particular, we reveal that two variant residues among SLC19 subfamily members designate the specificity for folate. Moreover, we identify intracellular thiamine pyrophosphate as the favorite coupled substrate for folate transport by SLC19A1. Together, this work establishes the molecular basis of substrate recognition by this central folate transporter.
Multistep diversification in spatiotemporal bacterial-phage coevolution
Authors: Shaer Tamar Einat , Kishony Roy
Published: December 2022

The evolutionary arms race between phages and bacteria, where bacteria evolve resistance to phages and phages retaliate with resistance-countering mutations, is a major driving force of molecular innovation and genetic diversification. Yet attempting to reproduce such ongoing retaliation dynamics in the lab has been challenging; laboratory coevolution experiments of phage and bacteria are typically performed in well-mixed environments and often lead to rapid stagnation with little genetic variability. Here, co-culturing motileE. coliwith the lytic bacteriophage T7 on swimming plates, we observe complex spatiotemporal dynamics with multiple genetically diversifying adaptive cycles. Systematically quantifying over 10,000 resistance-infectivity phenotypes between evolved bacteria and phage isolates, we observe diversification into multiple coexisting ecotypes showing a complex interaction network with both host-range expansion and host-switch tradeoffs. Whole-genome sequencing of these evolved phage and bacterial isolates revealed a rich set of adaptive mutations in multiple genetic pathways including in genes not previously linked with phage-bacteria interactions. Synthetically reconstructing these new mutations, we discover phage-general and phage-specific resistance phenotypes as well as a strong synergy with the more classically known phage-resistance mutations. These results highlight the importance of spatial structure and migration for driving phage-bacteria coevolution, providing a concrete system for revealing new molecular mechanisms across diverse phage-bacterial systems.
High-order tensor flow processing using integrated photonic circuits
Authors: Xu Shaofu , Wang Jing , Yi Sicheng , Zou Weiwen
Published: December 2022

Tensor analytics lays the mathematical basis for the prosperous promotion of multiway signal processing. To increase computing throughput, mainstream processors transform tensor convolutions into matrix multiplications to enhance the parallelism of computing. However, such order-reducing transformation produces data duplicates and consumes additional memory. Here, we propose an integrated photonic tensor flow processor (PTFP) without digitally duplicating the input data. It outputs the convolved tensor as the input tensor ‘flows’ through the processor. The hybrid manipulation of optical wavelengths, space dimensions, and time delay steps, enables the direct representation and processing of high-order tensors in the optical domain. In the proof-of-concept experiment, an integrated processor manipulating wavelengths and delay steps is implemented for demonstrating the key functionalities of PTFP. The multi-channel images and videos are processed at the modulation rate of 20 Gbaud. A convolutional neural network for video action recognition is demonstrated on the processor, which achieves an accuracy of 97.9%.
Three-dimensional wide-field fluorescence microscopy for transcranial mapping of cortical microcirculation
Authors: Zhou Quanyu , Chen Zhenyue , Liu Yu-Hang , El Amki Mohamad , Glück Chaim , Droux Jeanne , Reiss Michael , Weber Bruno , Wegener Susanne , Razansky Daniel
Published: December 2022

Wide-field fluorescence imaging is an indispensable tool for studying large-scale biodynamics. Limited space-bandwidth product and strong light diffusion make conventional implementations incapable of high-resolution mapping of fluorescence biodistribution in three dimensions. We introduce a volumetric wide-field fluorescence microscopy based on optical astigmatism combined with fluorescence source localization, covering 5.6×5.6×0.6 mm3 imaging volume. Two alternative configurations are proposed exploiting multifocal illumination or sparse localization of point emitters, which are herein seamlessly integrated in one system. We demonstrate real-time volumetric mapping of the murine cortical microcirculation at capillary resolution without employing cranial windows, thus simultaneously delivering quantitative perfusion information across both brain hemispheres. Morphological and functional changes of cerebral vascular networks are further investigated after an acute ischemic stroke, enabling cortex-wide observation of concurrent collateral recruitment events occurring on a sub-second scale. The reported technique thus offers a wealth of unmatched possibilities for non- or minimally invasive imaging of biodynamics across scales.
Changes in ocular biological parameters after cycloplegia based on dioptre, age and sex
Authors: Tao Yulin , Cheng Xiaokang , Ouyang Can , Qu Xiaoyong , Liao Wenjiang , Zhou Qiong , Ouyang Jun
Published: December 2022

The effects of cycloplegia on ocular biological parameters in children have been extensively studied, but few studies have compared these parameters between different refractive states, ages, and sexes. Therefore, the purpose of this study was to investigate the changes in ocular biometry before and after cycloplegia in different groups based on dioptre, age and sex. We examined a total of 2049 participants in this cross-sectional study. A comprehensive eye examination was conducted before cycloplegia. Cycloplegia was implemented with the application of atropine or tropicamide. Ocular biological parameters were evaluated after cycloplegia, including axial length (AL), mean keratometry (K), flat keratometry (K1), steep keratometry (K2), central corneal thickness (CCT), anterior chamber depth (ACD) and white-to-white (WTW) distance. All the participants were categorized based on dioptre, age and sex. Statistical analysis was performed with paired t tests and Wilcoxon signed-rank tests. Regarding dioptre, AL was found to be increased significantly in the Fs, Ast and FA (p < 0.05) postcycloplegia groups. We observed significant increases in K, K1, K2 and ACD in the Fs group (p < 0.05) after cycloplegia. Regarding age, we found significant increases in AL, CCT and ACD in group 1 (p < 0.05), but AL decreased significantly in groups 2 and 3 (p < 0.05) postcycloplegia. There were no significant changes found in K, K1 and K2 in the three groups after cycloplegia (p > 0.05). Regarding sex, AL and WTW were found to decrease significantly among males and increase significantly among females (p < 0.05) postcycloplegia, while K, K1 and K2 showed the opposite trends. This study showed that there were differences in some ocular biological parameters after cycloplegia across different groups; in particular, there were significant differences in AL, CCT and ACD. Attention should be devoted to the influence of cycloplegia in clinical work.
Dysregulation of TSP2-Rac1-WAVE2 axis in diabetic cells leads to cytoskeletal disorganization, increased cell stiffness, and dysfunction
Authors: Xing Hao , Huang Yaqing , Kunkemoeller Britta H. , Dahl Peter J. , Muraleetharan Ohvia , Malvankar Nikhil S. , Murrell Michael P. , Kyriakides Themis R.
Published: December 2022

Fibroblasts are a major cell population that perform critical functions in the wound healing process. In response to injury, they proliferate and migrate into the wound space, engaging in extracellular matrix (ECM) production, remodeling, and contraction. However, there is limited knowledge of how fibroblast functions are altered in diabetes. To address this gap, several state-of-the-art microscopy techniques were employed to investigate morphology, migration, ECM production, 2D traction, 3D contraction, and cell stiffness. Analysis of cell-derived matrix (CDM) revealed that diabetic fibroblasts produce thickened and less porous ECM that hindered migration of normal fibroblasts. In addition, diabetic fibroblasts were found to lose spindle-like shape, migrate slower, generate less traction force, exert limited 3D contractility, and have increased cell stiffness. These changes were due, in part, to a decreased level of active Rac1 and a lack of co-localization between F-actin and Waskott-Aldrich syndrome protein family verprolin homologous protein 2 (WAVE2). Interestingly, deletion of thrombospondin-2 (TSP2) in diabetic fibroblasts rescued these phenotypes and restored normal levels of active Rac1 and WAVE2-F-actin co-localization. These results provide a comprehensive view of the extent of diabetic fibroblast dysfunction, highlighting the regulatory role of the TSP2-Rac1-WAVE2-actin axis, and describing a new function of TSP2 in regulating cytoskeleton organization.
De-novo transcriptome assembly and analysis of lettuce plants grown under red, blue or white light
Authors: Kumar Vinod , Sugumaran Krishnakumar , Al-Roumi Amwaj , Shajan Anisha
Published: December 2022

Lettuce (Lactuca sativa) is grown in various parts of the world for use as a leafy vegetable. Although the use of light-emitting diode (LED) in controlled plant production systems has been successfully used to enhance nutritional quality and plant growth efficiently, the molecular basis of lettuce’s response to varying light spectra is not studied. Using next-generation sequencing, we have analyzed the transcriptomes of leaf lettuce (Lactuca sativa var. ‘New Red Fire’) grown hydroponically in a modular agricultural production system under three different types of LED lighting: red, blue, and white light. Illumina HiSeq sequencing platform was used to generate paired-end sequence reads (58 Gb raw and 54 Gb clean data) of the transcriptome of lettuce leaves exposed to varying light spectra. The de novo assembled final transcriptome contained 74,096 transcripts. Around 53% and 39% of the assembled transcripts matched to the UniProt and RefSeq RNA sequences, respectively. The validation of the differentially expressed transcripts using RT-qPCR showed complete agreement with RNA-Seq data for 27 transcripts. A comparison of the blue versus red light treatments showed the highest number of significantly differentially expressed transcripts. Among the transcripts significantly up-regulated in blue-light-exposed leaves compared to white-light-exposed leaves, ~ 26% were involved in the ‘response to stress’. Among the transcripts significantly upregulated under red light compared to white light, ~ 6% were associated with ‘nucleosome assembly’ and other processes, such as ‘oxidation–reduction process’ and ‘response to water deprivation’ were significantly enriched. Thus, the result from the current study provides deeper insights into differential gene expression patterns and associated functional aspects under varying light qualities.
Coumarin biosynthesis genes are required after foliar pathogen infection for the creation of a microbial soil-borne legacy that primes plants for SA-dependent defenses
Authors: Vismans Gilles , van Bentum Sietske , Spooren Jelle , Song Yang , Goossens Pim , Valls Josep , Snoek Basten L. , Thiombiano Benjamin , Schilder Mario , Dong Lemeng , Bouwmeester Harro J. , Pétriacq Pierre , Pieterse Corné M. J. , Bakker Peter A. H. M. , Berendsen Roeland L.
Published: December 2022

Plants deposit photosynthetically-fixed carbon in the rhizosphere, the thin soil layer directly around the root, thereby creating a hospitable environment for microbes. To manage the inhabitants of this nutrient-rich environment, plant roots exude and dynamically adjust microbe-attracting and -repelling compounds to stimulate specific members of the microbiome. Previously, we demonstrated that foliar infection of Arabidopsis thaliana by the biotrophic downy mildew pathogen Hyaloperonospora arabidopsidis (Hpa) leads to a disease-induced modification of the rhizosphere microbiome. Soil conditioned with Hpa-infected plants provided enhanced protection against foliar downy mildew infection in a subsequent population of plants, a phenomenon dubbed the soil-borne legacy (SBL). Here, we show that for the creation of the SBL, plant-produced coumarins play a prominent role as coumarin-deficient myb72 and f6’h1 mutants were defective in creating a Hpa-induced SBL. Root exudation profiles changed significantly in Col-0 upon foliar Hpa infection, and this was accompanied by a compositional shift in the root microbiome that was significantly different from microbial shifts occurring on roots of Hpa-infected coumarin-deficient mutants. Our data further show that the Hpa-induced SBL primes Col-0 plants growing in SBL-conditioned soil for salicylic acid (SA)-dependent defenses. The SA-signaling mutants sid2 and npr1 were unresponsive to the Hpa-induced SBL, suggesting that the protective effect of the Hpa-induced shift in the root microbiome results from an induced systemic resistance that requires SA-signaling in the plant.
Current distribution monitoring enables quench and damage detection in superconducting fusion magnets
Authors: Teyber Reed , Weiss Jeremy , Marchevsky Maxim , Prestemon Soren , van der Laan Danko
Published: December 2022

Fusion magnets made from high temperature superconducting ReBCO CORC® cables are typically protected with quench detection systems that use voltage or temperature measurements to trigger current extraction processes. Although small coils with low inductances have been demonstrated, magnet protection remains a challenge and magnets are typically operated with little knowledge of the intrinsic performance parameters. We propose a protection framework based on current distribution monitoring in fusion cables with limited inter-cable current sharing. By employing inverse Biot-Savart techniques to distributed Hall probe arrays around CORC® Cable-In-Conduit-Conductor (CICC) terminations, individual cable currents are recreated and used to extract the parameters of a predictive model. These parameters are shown to be of value for detecting conductor damage and defining safe magnet operating limits. The trained model is then used to predict cable current distributions in real-time, and departures between predictions and inverse Biot-Savart recreated current distributions are used to generate quench triggers. The methodology shows promise for quality control, operational planning and real-time quench detection in bundled CORC® cables for compact fusion reactors.
Investigation of anticoagulant rodenticide resistance induced by Vkorc1 mutations in rodents in Lebanon
Authors: Rached Antoine , Abi Rizk Georges , Mahamat Ali Barka , Khoury Graziella El , El Hage Jeanne , Harran Elena , Lattard Virginie
Published: December 2022

Anticoagulant rodenticides (AR) remain the most effective chemical substances used to control rodents in order to limit their agricultural and public health damage in both rural and urban environments. The emergence of genetically based resistance to AR worldwide has threatened effective rodent control. This study gives a first overview of the distribution and frequency of single nucleotide polymorphism in the vitamin K epoxide reductase subcomponent 1 (Vkorc1) gene in rodents in Lebanon. In the Mus genus, we detected two missense mutations Leu128Ser and Tyr139Cys, that confer resistance to anticoagulant rodenticides in house mice and a new missense mutation Ala72Val in the Mus macedonicus species, not previously described. In the Rattus genus, we found one missense mutation Leu90Ile in the roof rat and one missense mutation Ser149Ile in the Norway rat. This is the first study to demonstrate potential resistance to AR in Lebanese rodents and therefore it provides data to pest control practitioners to choose the most suitable AR to control rodents in order to keep their efficacy.
The projected burden of non-communicable diseases attributable to overweight in Brazil from 2021 to 2030
Authors: Nilson Eduardo A. F. , Gianicchi Beatriz , Ferrari Gerson , Rezende Leandro F. M.
Published: December 2022

Although studies have quantified the current burden of diseases attributable to overweight and obesity in Brazil, none have estimated its burden in the future. The study aimed to estimate the potential impact of different scenarios of changes in the prevalence of overweight on non-communicable diseases (NCD) in the Brazilian adult population until 2030. We developed a multistate life table model including 11 body mass index (BMI) related diseases to estimate attributable NCDs cases and deaths under the following scenarios of changes in overweight over a 10-year simulation: (1) the continuity of the current trajectory of BMI increases, (2) reducing the rate of increase by half, (3) stopping future BMI increases, and (4) the reduction of the prevalence of overweight by 6.7%. In Brazil, if the current trends of BMI increase are maintained from 2021 to 2030, approximately 5.26 million incident cases and 808.6 thousand deaths from NCDs may occur due to overweight. If the annual increase in overweight was reduced by half until 2030, 1.1% of new NCD cases and 0.2% of deaths could be prevented (respectively, 29,600 cases and 1900 deaths). Alternatively, if the current prevalence of overweight is maintained, as set as a national goal in Brazil until 2030, the incident NCD cases and the deaths could be reduced by respectively 3.3% (92,900) and 1.5% (12,100) compared to continuation of current trends. If the prevalence of overweight is reduced by 6.7% until 2030, 6.5% (182,200) of NCD cases and 4.2% (33,900) of deaths could be prevented. The epidemiologic burden of overweight in Brazil tends to increase if bold policy interventions are not adopted in Brazil.
RF energy harvesting schemes for intelligent reflecting surface-aided cognitive radio sensor networks
Authors: Wang Jihong , Yu Hongquan
Published: December 2022

Energy harvesting (EH) is a potential solution to enhance the node sustainability and prolong the network lifetime of cognitive radio sensor networks (CRSNs). However, CRSNs nodes can only harvest energy from the direct link with energy sources, and severe path loss results in low energy utilization ratio. To solve the above problem, intelligent reflecting surface (IRS) is introduced, and a shared reflection coefficient matrix-based EH scheme is proposed for IRS-aided CRSNs in this paper. An optimization problem with the objective of maximizing the total amount of energy harvested by all CRSNs nodes is formulated, and by optimally adjusting the IRS reflection coefficient, CRSNs nodes can harvest energy from both the direct link and the cascaded reflection link via IRS, which increases the amount of harvested energy. In addition, a subsurface partition-based EH scheme is proposed to reduce the additional computational complexity brought by increasing IRS elements or CRSNs nodes. Simulation results show that the proposed schemes can both dramatically improve energy utilization ratio, and the subsurface partition-based EH scheme will bring in less than 1 percent performance loss when compared with the other scheme, i.e., reasonable subsurface partition can achieve a balance between harvested energy and computational complexity.
A sputum bioassay for airway eosinophilia using an eosinophil peroxidase aptamer
Authors: Ali M. Monsur , Wolfe Michael G. , Mukherjee Manali , Radford Katherine , Patel Zil , White Dawn , Milojevic Julijana , Capretta Alfredo , Nair Parameswaran , Brennan John D.
Published: December 2022

Eosinophils are granulocytes that play a significant role in the pathogenesis of asthma and other airway diseases. Directing patient treatment based on the level of eosinophilia has been shown to be extremely effective in reducing exacerbations and therefore has tremendous potential as a routine clinical test. Herein, we describe the in vitro selection and optimization of DNA aptamers that bind to eosinophil peroxidase (EPX), a protein biomarker unique to eosinophils. Fifteen rounds of magnetic bead aptamer selection were performed prior to high throughput DNA sequencing. The top 10 aptamer candidates were assessed for EPX binding using a mobility shift assay. This process identified a lead aptamer candidate termed EAP1-05 with low nanomolar affinity and high specificity for EPX over other common sputum proteins. This aptamer sequence was further optimized through truncation and used to develop an easy-to-use colourimetric pull-down assay that can detect EPX over a concentration range from 1 – 100 nM in processed sputum. Forty-six clinical samples were processed using a new sputum dispersal method, appropriate for a rapid assessment assay, that avoids centrifugation and lengthy processing times. The assay showed 89% sensitivity and 96% specificity to detect eosinophilia (compared to gold standard sputum cytometry), with results being produced in under an hour. This assay could allow for an easy assessment of eosinophil activity in the airway to guide anti-inflammatory therapy for several airway diseases.
Supplementation of Alpha-lipoic acid-loaded nanoliposomes in semen extender improves freezability of buffalo spermatozoa
Authors: Hassan Mahmoud A. E. , Khalil Wael A. , Abdelnour Sameh A. , Aman Reham Mokhtar
Published: December 2022

This research was designed to explore the protective effect of alpha-lipoic acid–loaded nanoliposomes (ALAN) during cryopreservation of buffalo sperm. Buffalo semen was cryopreserved in a tris-citrate egg yolk extender without any supplement (ALAN0, control group) or with ALAN at levels of 25, 50, 75 or 150 µg (ALAN25, ALAN50, ALAN75 and ALAN150, respectively). The ALAN had a size of 171.80 nm and a negative zeta potential (− 43.40 mV). The progressive motility, vitality and membrane integrity significantly improved in all ALAN groups (except ALAN25 for membrane integrity). ALAN150 group exhibited the best values of progressive sperm motility, vitality and membrane integrity after thawing at 37 °C for 30 s or incubated for 2 h at 37 °C and 5% CO2 compared with those in other groups. Both ALAN75 and ALAN150 groups significantly improved the TAC, GR and catalase, while lipid peroxidation and early apoptotic spermatozoa significantly decreased in ALAN150 group followed by ALAN75 group. Collectively, the adding ALAN to buffalo semen freezing extender plays a substantial shielding function against cryodamage by preserving the sperm functional parameters.