bluuebunny/crossref_metadata_2025 huggingface dataset:
Temporal trends and outcomes of critical limb ischemia among patients with chronic kidney disease
Authors: Elbadawi Ayman , Elgendy Islam Y , Megaly Michael , Elzeneini Mohammed , Mentias Amgad , Omer Mohamed , Ogunbayo Gbolahan , Rai Devesh , Drachman Douglas E , Shishehbor Mehdi H
Published: October 2020

There is a paucity of data on the outcomes and revascularization strategies for critical limb ischemia (CLI) among patients with chronic kidney disease (CKD). Hence, we conducted a nationwide analysis to evaluate the trends and outcomes of hospitalizations for CLI with CKD. The National Inpatient Sample database (2002–2015) was queried for hospitalizations for CLI. The trends of hospitalizations for CLI with CKD were reported, and endovascular versus surgical revascularization strategies for CLI with CKD were compared. The main study outcome was in-hospital mortality. The analysis included 2,139,640 hospitalizations for CLI, of which 484,224 (22.6%) had CKD. There was an increase in hospitalizations for CLI with CKD (Ptrend = 0.01), but a reduction in hospitalizations for CLI without CKD (Ptrend = 0.01). Patients with CLI and CKD were less likely to undergo revascularization compared with patients without CKD. CLI with CKD had higher rates of in-hospital mortality (4.8% vs 2.5%, adjusted odds ratio (OR) 2.01; 95% CI 1.93–2.11) and major amputation compared with no CKD. Revascularization for CLI with CKD was associated with lower rates of mortality (3.7% vs 5.3%, adjusted-OR 0.78; 95% CI 0.72–0.84) and major amputation compared with no revascularization. Compared with endovascular revascularization, surgical revascularization for CLI with CKD was associated with higher rates of in-hospital mortality (4.7% vs 2.7%, adjusted-OR 1.67; 95% CI 1.43–1.94). In conclusion, this contemporary observational analysis showed an increase in hospitalizations for CLI among patients with CKD. CLI with CKD was associated with higher in-hospital mortality compared with no CKD. Compared with endovascular therapy, surgical revascularization for CLI with CKD was associated with higher in-hospital mortality.
Morts pour la France: Things and memory in the ‘destroyed villages’ of Verdun
Authors: Filippucci Paola
Published: October 2020

This article considers the power of things to affect how the past is remembered in the aftermath of mass violence, through the case of the ‘destroyed villages’ ( villages détruits) of the battlefield of Verdun, theatre in 1916 of one of the most destructive battles of World War I. As well as causing mass military death, the battle also led to the ‘death’ of nine small villages, declared to have ‘died for France’ and incorporated into the post-war commemorative landscape of the battlefield. The article illustrates the 21st-century discourse and practices that surround the remains of these villages, from emplaced ruins to photographs and other documents. A century after the ‘death’ of the villages, people who identify as descendants of the original inhabitants gather at the sites and through these objects evoke their ancestors and the pre-war settlement, momentarily reconstituting a space that they can ‘inhabit’ physically, imaginatively and affectively. However, bids to restore a ‘village’ space and time are overwritten by the commemorative framework in which the sites and remains have been embedded for the past century, that identifies the ‘dead’ localities with the human Fallen and their history with the moment of their ‘death for France’. So, while the surviving traces of the former villages retain their power to affect and thus to evoke the pre-war, civilian past, their ability to produce a new memory for Verdun is limited by their incorporation into a memorial landscape dedicated to heroic military death for the nation. The physical expropriation of sites and vestiges during the post-war reconstruction of the battlefield and their preservation as tangible tokens of mass death has enduringly fixed and overdetermined their meaning, in a form of symbolic expropriation that limits their power to produce memory.
Doing paying during the Covid-19 pandemic
Authors: Mondada Lorenza , Bänninger Julia , Bouaouina Sofian A , Gauthier Guillaume , Hänggi Philipp , Koda Mizuki , Svensson Hanna , Tekin Burak S
Published: October 2020

The Covid-19 pandemic has affected not only the health of populations but also their everyday social practices, transformed by orienting to risks of contagion and to health prevention discourses. This paper emanates from a project investigating the impact of Covid-19 on human sociality and more particularly the situated and embodied organization of social interactions. It discusses how Covid-19 impacts the design of ordinary actions in social interaction, how this is made publicly accountable by the participants orienting to the pandemic in formatting their actions and in responding to the actions of others. Adopting an ethnomethodological and conversation analytic perspective, the analyses focus on a particular social activity: paying. The organization of payments in shops and services has been affected by the pandemic, not only by official regulations, favoring some modes of payment over others, but also in how sellers and customers situatedly adapt their practices to imperatives of prevention. On the basis of a rich corpus of video-recorded data, which spans from the pandemic’s prodromes to and after its peak, we show how money transfer is methodically achieved – imposed, negotiated, and readjusted – while variously taking into account possible risks of contagion. Thus, we show not only how pandemics affect social interaction, and how prevention is incarnated in social actions, but also how, in turn, situated solutions implemented by people during the pandemic reveal fundamental features of human action.
Informal landscapes and the performative placing of insurgent planning
Authors: Sletto Bjørn
Published: October 2020

Non-representational theory illuminates the role of mundane, performative presentations in the production of emotional geographies, while drawing attention to the unexpected (the event, the encounter, the disturbance) which challenge hegemonic representations of landscapes. This focus on situated and performative entanglement of social and material relations has important implications for insurgent planning practice and theory. Such creative productions of emotional geographies, grounded in particular places and socialities, may foster new imaginaries and generate new and innovative spaces of engagement and oppositional planning practices. Drawing on performativity and non-representational theory, this article proposes a reimagining of insurgent planning that takes into account everyday practices and meaning-making but also the materiality of landscape in particular places. The theoretical discussion draws on the example of household plant production and vermicomposting in Los Platanitos, Santo Domingo Norte, Dominican Republic. The article describes the performative relationships between people, plants, and worms in Los Platanitos, examining the ways in which this relationality serves as a locus for insurgent planning practices in the face of a major stormwater project.
Teacher Leadership Development: Building Bridges not Borders between Israeli Jewish and Palestinian Educators
Authors: Jabr Dajani Dua , Katz-Berger Hila , King M. Bruce , Lang Laura , Levy Ariel , Pulvermacher Yael
Published: October 2020

Teacher leadership development receives considerable attention in many educational reforms across the globe. This article reports on a unique partnership in Jerusalem that brings Israeli and Palestinian educators together to cultivate teacher leaders who facilitate professional communities and support continual improvement in teaching and learning. The research design involves participatory action research and draws on theoretical frameworks of democratic education, productive professional discourse, and authentic intellectual work. Findings focus on the enhancement of multicultural training, increased depth of pedagogical discussions, and improvement in leading teacher learning communities. The conclusions consider the ways in which a few bridges are overcoming both real and perceived borders in a region of persisting cultural tension and conflict, as the teacher leaders and co-authors developed an emerging common understanding of a shared conception of professional practice across three languages, and a growing mutual respect for the ‘other’.
Women in Solitary Confinement: Relationships, Pseudofamilies, and the Limits of Control
Authors: Aranda-Hughes Vivian , Turanovic Jillian J. , Mears Daniel P. , Pesta George B.
Published: October 2020

Drawing on qualitative data from focus groups with correctional personnel in one of the nation’s largest women’s prisons, this study examines staff perceptions of how incarcerated women cope with long-term solitary confinement. We find that women’s strong ties to other women and their prison pseudofamilies may influence the behaviors that explain their placement and stays in solitary confinement. We find, too, that women are perceived to go to extreme lengths to build and maintain relationships with other women. The findings showcase unintended consequences of solitary confinement, raise questions about its effectiveness, and highlight the limits of institutional control.
Sources of Information for Learning and Decision-Making in Men With Localized Prostate Cancer
Authors: Chhatre Sumedha , Wittink Marsha N. , Gallo Joseph J. , Jayadevappa Ravishankar
Published: October 2020

Information seeking is essential for effective patient-centered decision-making. However, prostate cancer patients report a gap between information needed and information received. The importance of different information sources for treatment decision remains unclear. Thus, using the Comprehensive Model of Health Information (CMIS) framework, we assessed the antecedent factors, information carrier factors, and information-seeking activities in localized prostate cancer patients. Data were collected via semistructured one-on-one, interviews and structured survey. Men with localized prostate cancer were recruited from two urban health-care centers. Following the interview, participants completed a survey about sources that were helpful in learning about prostate cancer treatment and decision-making. The interviews were audio-recorded, transcribed, and subjected to a thematic analysis using NVivo 10. Fifty localized prostate cancer survivors completed the interviews and surveys. Important antecedent factors that were observed were age, marital status, uncertainty, anxiety, caregiver burden, and out-of-pocket expenses. We identified complexity, magnitude, and reliability as information carrier characteristics. Preferred sources for information were health providers, medical websites, and pamphlets from the doctor’s office. These sources were also perceived as most helpful for decision-making. Urologists, urological oncologists, and radiation/radiation oncologists were important sources of information and helpful in decision-making. Prostate cancer patients obtained information from multiple sources. Most prostate cancer patients make patient-centered choices by incorporating personal factors and medical information. By considering factors that influence patients’ treatment decisions, health-care providers can enhance the patient-centeredness of care. Multiple strategies and interventions are necessary for disseminating valid, reliable, and unbiased information to prostate cancer patients to facilitate informed decisions.
Responsibility sharing for adolescents with type 1 diabetes: A scoping review
Authors: Gardener Lisa , Desha Laura , Bourke-Taylor Helen , Ziviani Jenny
Published: October 2020

Background

The term ‘Responsibility Sharing’, albeit poorly defined, has emerged from the diabetes literature, to describe a distinct mechanism for comprehensively managing the characteristic shift in responsibility that underpins the transition to self-management for adolescents.

Methods

A scoping review, following the PRISMA-ScR guidelines, distilled the literature from seven databases to answer the questions: What is responsibility sharing? Who are the key stakeholders? What factors affect responsibility transaction? What are its recognized outcomes? How is responsibility shared?

Results

Responsibility sharing is a transactional arrangement between youth and their caregiver/s that functions to repeatedly and flexibly apply ownership to the management of diabetes care tasks, across the course of adolescence. In the main, responsibility sharing was associated with better metabolic and/or psychosocial outcomes. Effective responsibility sharing was seen as being responsive to adolescent capacity and driven by autonomy supportive, sustained communication patterns that enable mutually agreeable responsibility assumption by all stakeholders.

Conclusion

Different perspectives on responsibility sharing for adolescents with Type 1 diabetes, and the lack of a universal definition, have led to discordance within the literature about its operationalization and measurement. This paper proposes a definition of responsibility sharing for future researchers to apply.
Drugs, Death, Denial and Cancer Care: Using Breaking Bad in the spiritual care of cancer patients
Authors: Bowlby Ewan
Published: October 2020

This article argues that watching Breaking Bad (2008–13) could encourage people affected by cancer to recognise and reconsider damaging reactions to their condition. If viewers are invited to see the series’ antihero, Walter White, as an iconic ‘silhouette’ of a better path not taken, this can provoke them to entertain more honest, constructive attitudes to cancer and death. Using the theological concept of a ‘silhouette of goodness’ and Jung’s theory of the ego-life and true Self, this article suggests that symbolic moments in Walt’s descent into chaotic criminality could help caregivers to meet the ‘need for symbols’ in cancer care.
Crossing the Borders of Queer TV: Depictions of migration and (im)mobility in contemporary LGBTQ television
Authors: Horvat Anamarija
Published: October 2020

This article focuses on contemporary queer television and examines its depiction of LGBTQ border crossings and migration. In recent years, a shift in the American and British televisual landscape has seen queer television drama veering away from a predominant focus on white, middle-class characters, towards an exploration of immigrant positionalities and the geopolitical relevance of state borders. However, these changes in media representation have not yet been granted sufficient academic attention. This article aims to fill this gap through analysing three relevant examples of US and UK queer television; Transparent (2014–2019) , Years and Years (2019) and Orange is the New Black (2013–2019).
Introduction: Trans TV dossier, III: Trans TV re-evaluated, part 2
Authors: Goddard Edited and introduced by Michael , Hogg Christopher
Published: October 2020

This is the second more substantial part of the introduction to the final Trans TV special issue, distributed across issues 15.2 and 15.3 of this journal. As well as introducing the contents of this issue, we reflect in this introduction on the Trans TV project as it has developed since 2017, via an engagement with two key televisual texts namely Transparent (2014–2019) and Pose (2018–). We argue that this certainly reflects positive developments in terms of transgender representation and authorship but this progress is more complex when considered in terms of television aesthetics and politics. We also propose, drawing on the work of Koch-Rein et al (2020), a shift form representation towards the concept of ‘transing’ as a reading strategy, and argue that this has been at the heart of the Trans TV project all along.
Agents of memory in the post-witness era: Memory in the Living Room and changing forms of Holocaust remembrance in Israel
Authors: Kook Rebecca
Published: October 2020

With the passing of the survivors of the Holocaust and the aging of the second generation, new agents and initiatives are transforming the commemorative landscape of Holocaust remembrance. This article examines the impact of this generational transition on the production of collective memory of the Holocaust with focus on a new remembrance project in Israel, known as Memory in the Living Room. While some attention has been paid to its innovative structure and anti-paradigmatic components, none has focused on its agents and their mnemonic agenda. This paper argues that an examination of this agenda and an exploration of the wider structural contexts within which it developed are crucial to a deeper understanding of its overall significance. Based on 20 in-depth interviews the article argues that the emergent commemorative agenda is a hybrid version of transnational memory informed by concepts and practices drawn from a global digitalized culture.
Complementarities as an antitrust defense
Authors: Weisman Dennis L.
Published: October 2020

We employ a Cournot model with interdependent demands to explore the interaction between demand and cost complementarities in mitigating upward pricing pressure, post-merger. The analysis reveals that even substantial increases in the HHI post-merger need not raise competitive concerns when output is redistributed from single-market to multi-market providers. Furthermore, the numerical simulations indicate that there is a wide range of demand and cost complementarity parameters over which even monopolization of the market would not be expected to result in higher prices. These findings may constructively inform merger policy and provide useful context for application of the DOJ/FTC horizontal merger guidelines in an increasingly digitized (network) economy.
Conservation and Management of Trachemys venusta venusta in Southern Mexico: A Genetic Approach
Authors: Recino-Reyes Elsi Beatriz , Lesher-Gordillo Julia María , Machkour-M’Rabet Salima , Gallardo-Alvarez Manuel Ignacio , Zenteno-Ruiz Claudia Elena , Olivera-Gómez León David , Valdés-Marín Alejandra , Gómez-Carrasco Guadalupe , Ríos-Rodas Liliana , Barragán-Vázquez María del Rosario , Martínez Raymundo Hernández
Published: October 2020

The Meso-American slider turtle ( Trachemys venusta) is a freshwater turtle that is widely distributed from Mexico to Colombia. Due to the overexploitation of populations of this species in Mexico, it has been placed within the “subject to special protection” category formulated by the Official Mexican Standard NOM-059-ECOL-2010. In the state of Tabasco, Mexico, Management Units for the Conservation of Wildlife (UMA) were created to reduce the impact of overexploitation of freshwater turtles bred in captivity. However, no genetic management plan was considered. The present study was carried out in an UMA in the state of Tabasco. We obtained the level of genetic diversity of the founder individuals of the UMA in order to develop a management plan which will optimize reproduction in the UMA. Genetic diversity was compared between captive (n = 86) and wild (n = 45) individuals using 14 microsatellite molecular markers. The genetic diversity parameter ​​determined in this study was slightly higher for captive than for wild population ( He = 0.606 and He = 0.594 respectively), reflecting the mix of genetic sources in captive group (founding individuals from different localities) and demonstrating that the captive population contains a diverse subset of alleles from representative populations. The analysis of genetic structure revealed a relationship between captive and wild populations, indicating the influence of the two principal river basins in this region on the populations structure of freshwater turtles. Finally, according to the results obtained from the relationship analysis, we recommend the use of 19 females and 13 males to constitute the appropriate breeding group, generating a potential of 247 dyads with no relationship. However, in order to improve breeding program and the genetic diversity of captive population, we suggest to introduce wild-caught individuals. These results are the first regarding genetic management in a Mexican UMA and demonstrate the importance of molecular approaches in the management and conservation of captive species.
COVID-19: Planning and Postpandemic Partnerships
Authors: Bullard Jodi S. , McAlister Barbara S. , Chilton Jenifer M.
Published: October 2020

The emergence of coronavirus disease 2019 (COVID-19) has highlighted the need for strong partnerships between educators and healthcare professionals to facilitate the reopening of schools. School nurses are uniquely positioned to bridge this gap because of their role as a healthcare professional in the educational setting. Past research identifies the effectiveness of collaborative efforts to serve students by community partners, school system personnel, and school nurses. However, partnerships have been episodic and reactive rather than ongoing and proactive. The circumstances of COVID-19 present an opportunity for school nurses to promote collaboration and planning as an ongoing practice. Recommendations for school nurses to form ongoing public health and school system partnerships, as well as with teachers, parents, and school nursing colleagues, are presented.
Poricoic acid A as a modulator of TPH-1 expression inhibits renal fibrosis via modulating protein stability of β-catenin and β-catenin-mediated transcription
Authors: Chen Dan-Qian , Wu Xia-Qing , Chen Lin , Hu He-He , Wang Yan-Ni , Zhao Ying-Yong
Published: October 2020

Background:

Renal fibrosis is the common feature of chronic kidney disease (CKD). However, few drugs specifically target fibrogenesis due to the lack of an effective therapeutic target. Hence, it is urgent to find a therapeutic strategy that inhibits renal fibrosis. Here, we identified that poricoic acid A (PAA) as the modulator of tryptophan hydroxylase-1 (TPH-1), the key enzyme in tryptophan metabolism, exerted potent anti-fibrotic effects in the kidney.

Methods:

Lentiviral vector, luciferase reporter activity assay and co-immunoprecipitation were used. The animal model of unilateral ureteral obstruction and adenine-induced chronic renal failure as well as transforming growth factor (TGF)-β1-treated epithelial cells NRK-52E and fibroblasts NRK-49F were used.

Results:

TPH-1 was gradually decreased during CKD progression, while PAA treatment significantly increased TPH-1 expression to suppress renal fibrosis. Pharmacological overexpression of TPH-1 by PAA treatment exhibited anti-fibrosis and was linked to Wnt/β-catenin signaling activity. TPH-1 exhibited anti-fibrotic effects by suppressing epithelial cell injury and fibroblast activation, and PAA promoted TPH-1 expression and then suppressed the Wnt/β-catenin signaling pathway via regulating the protein stability of β-catenin and β-catenin-mediated transcription. TPH-1 overexpression enhanced the anti-fibrotic effects of PAA, while TPH-1 deficiency weakened the anti-fibrotic effects of PAA, indicating that TPH-1 was required for the anti-fibrotic effects of PAA.

Conclusion:

PAA as a modulator of TPH-1 expression attenuated renal fibrosis through regulating the Wnt/β-catenin signaling pathway by acting on the protein stability of β-catenin and β-catenin-mediated transcription. TPH-1 was required for PAA to exert anti-fibrosis.
Polymorphisms in the 5’-UTR region of IL-10RA gene are associated with chronic kidney disease
Authors: Hwang Eunah
Published: October 2020

Chronic kidney disease (CKD), characterized by the progressive, irreversible loss of kidney function, has been a significant socioeconomic burden. Thus, identifying potential treatment targets and incipient biological risk indicators for CKD prediction is crucial. Recently several studies revealed that IL-10, IL-10RA, and IL-10RB genes were involved in the development of vascular complications in hypertension and stroke. The purpose of this study was to evaluate the association between CKD and IL-10, IL-10RA, and IL-10RB gene polymorphisms in the Korean population. Ninety-two CKD patients and 312 control subjects participated in the study. Blood samples were drawn for biochemical measurements and genetic polymorphism analysis. To analyze the genotypes of each single nucleotide polymorphism (SNP), PCR products were sequenced by a DNA analyzer. The four SNPs of the IL-10RA gene showed significant associations between CKD and the control group in the codominant1 (rs2228054, rs2228055, and rs9610, OR = 0.48, 95% CI = 0.28–0.82, p = 0.007; rs2256111, OR = 0.49, 95% CI = 0.29–0.83, p = 0.008, respectively), dominant (rs2228054, rs2228055, and rs9610, OR = 0.59, 95% CI = 0.36–0.95, p = 0.028; rs2256111, OR = 0.60, 95% CI = 0.38–0.97, p = 0.037, respectively), and over-dominant model (rs2228054 and rs2228055, OR = 0.47, 95% CI = 0.28–0.79, p = 0.0034; rs9610, OR = 0.48, 95% CI = 0.29–0.80, p = 0.0037; rs2256111, OR = 0.49, 95% CI = 0.29–0.80, p = 0.0039). The four SNPs of IL-10 showed significant associations with the estimated glomerular filtration rate (eGFR) and C-reactive protein (CRP), with the minor homozygote genotype of each SNP associated with lower eGFRs and decreased CRP levels ( p < 0.05, p = 0.001, respectively). The two SNPs of IL-10RA showed significant associations with the CRP levels ( p < 0.05). The two SNPs of IL-10RB were significantly associated with the eGFR, with the minor homozygote genotype of each SNP associated with lower eGFRs ( p < 0.01). Our results show that IL-10RA gene variants are associated with CKD development. Additionally, this study suggests that SNPs of IL-10RA were associated with CRP levels in CKD.
Provision and Perception of Physiotherapy in the Nonoperative Management of Degenerative Cervical Myelopathy (DCM): A Cross-Sectional Questionnaire of People Living With DCM
Authors: Butler Max B. , Mowforth Oliver D. , Badran Abdul , Starkey Michelle , Boerger Timothy , Sadler Iwan , Tabrah Julia , Treanor Caroline , Phys Lucy Cameron Grad Dip , Kalsi-Ryan Sukhvinder , Laing Rodney J. , Davies Benjamin M. , Kotter Mark R. N.
Published: October 2020

Study Design:

Cross-sectional survey.

Objectives:

Degenerative cervical myelopathy (DCM) is a common syndrome of acquired spinal cord impairment caused by canal stenosis secondary to arthritic changes of the spine. International guidelines consider physiotherapy an option for mild, stable DCM; however, few studies have been conducted on nonoperative management. The objective was to determine current usage and perceptions of nonoperative physiotherapy for DCM.

Methods:

Persons with DCM were recruited to a web-based survey. Participants with complete responses that had not received surgery were included (n = 167). Variables included symptom duration, treatment history, current disability, and demographic characteristics.

Results:

Disease and demographic characteristics were equivalent between those who did and did not receive physiotherapy. In all, 19.5% of physiotherapy recipients reported subjective benefit from physiotherapy. Those perceiving benefit had significantly higher mJOA (modified Japanese Orthopaedic Association) scores, lower neck pain scores, and shorter symptom duration. In multivariate logistic regression analysis, those with mild DCM were more likely to perceive benefit than those with severe DCM, as were those with moderate DCM (to a lesser extent). Individuals whose diagnosis was delayed 1 to 2 years were less likely to perceive benefit than those that waited 0 to 6 months.

Conclusions:

The provision of nonoperative physiotherapy in the management of DCM is inconsistent and appears to differ from international guidelines. Few patients perceived benefit from physiotherapy; however, this was more likely in those with mild DCM and in those with shorter symptom durations. Further work is needed to establish the appropriate role of physiotherapy for this population.
How Walkable Neighborhoods Promote Physical Activity: Policy Implications for Development and Renewal
Authors: Balcetis Emily , Cole Shana , Duncan Dustin T.
Published: October 2020

Mitigating physical inactivity is vital for public health. Neighborhoods that include visually engaging, eye-catching objects and locations increase the frequency, duration, and vigorousness of resident and visitors’ exercise. Three findings are key: First, individuals in neighborhoods that include features directly relevant to exercise—including dense mixed-use developments, greenspaces, parks, sidewalks, and connected streets—are more active and maintain better health. Second, when neighborhoods include visually interesting contents that are indirectly relevant to exercise, individuals believe exercise is more feasible, and this change in psychological mindset predicts increased physical activity. Third, as individuals become more physically active, they are less tempted by unhealthy food, which may counteract the detrimental effects on healthy eating that having proximal fast-food restaurants in neighborhoods poses. Race and socioeconomic disparities co-exist with the contents of neighborhoods. We highlight implications for urban planners, developers, community groups, and individuals selecting and designing public spaces that are conducive to healthy lifestyles.
Feedback Between Psychological Science and Policy in the Context of Same-Sex Couples
Authors: Fingerhut Adam W. , Frost David M.
Published: October 2020

Psychological science informed recent policy changes granting increased rights for same-sex couples. Understanding that the link between science and policy goes both ways, how should policy inform the next generation of research concerning same-sex couples and sexual minority individuals? This article presents ways that psychological research influenced marriage policy and then puts forth suggestions for future research for the attention of scholars, funders, and policymakers. These include examinations of minority stress in the age of marriage equality; new stressors for same-sex couples including legal divorce; relationship expectations and experiences for emerging adults; and the potential impact of marriage equality for mixed-sex couples including a further challenge to strict gender roles in marriage. The article ends by acknowledging the need for continued application of research to emerging policy questions affecting same-sex couples and sexual minority individuals.
Interracial Contact in Virtual Reality: Best Practices
Authors: Taylor Valerie Jones , Valladares Juan José , Siepser Claire , Yantis Caitlyn
Published: October 2020

Interracial interactions are occurring more frequently in virtual reality (VR) environments (e.g., multiplayer games, virtual classrooms, medical appointments). Individuals bring their biases and prejudices with them as they enter virtual spaces. Drawing from theories of real-world interracial interaction, predictable affective, cognitive, and behavioral processes undermine the quality of live interracial interactions. Emerging research using VR suggests that these same challenges occur in virtual social interactions. These challenges can be addressed using evidence-based best practices and policy recommendations: Improving racial/ethnic representation in VR, regulating codes of conduct, ensuring transparency about privacy/consent, and reducing explicit and implicit racist behavior. Following these recommendations can improve VR interracial contact and, in turn, real-world interracial interactions. While VR can serve as a unique tool to bring people together, carefully considered policies must mitigate the negative effects of racial bias that can hinder the progress of improved interracial interactions.
Medial entorhinal cortex lesions induce degradation of CA1 place cell firing stability when self-motion information is used
Authors: Jacob Pierre-Yves , Van Cauter Tiffany , Poucet Bruno , Sargolini Francesca , Save Etienne
Published: October 2020

The entorhinal–hippocampus network plays a central role in navigation and episodic memory formation. To investigate these interactions, we examined the effect of medial entorhinal cortex lesions on hippocampal place cell activity. Since the medial entorhinal cortex is suggested to play a role in the processing of self-motion information, we hypothesised that such processing would be necessary for maintaining stable place fields in the absence of environmental cues. Place cells were recorded as medial entorhinal cortex–lesioned rats explored a circular arena during five 16-min sessions comprising a baseline session with all sensory inputs available followed by four sessions during which environmental (i.e. visual, olfactory, tactile) cues were progressively reduced to the point that animals could rely exclusively on self-motion cues to maintain stable place fields. We found that place field stability and a number of place cell firing properties were affected by medial entorhinal cortex lesions in the baseline session. When rats were forced to rely exclusively on self-motion cues, within-session place field stability was dramatically decreased in medial entorhinal cortex rats relative to SHAM rats. These results support a major role of the medial entorhinal cortex in processing self-motion cues, with this information being conveyed to the hippocampus to help anchor and maintain a stable spatial representation during movement.
Metabolism in tumour-associated macrophages: aquid pro quowith the tumour microenvironment
Authors: Zheng Xiang , Mansouri Siavash , Krager Annika , Grimminger Friedrich , Seeger Werner , Pullamsetti Soni S. , Wheelock Craig E. , Savai Rajkumar
Published: October 2020

Lung cancer is the leading cause of death from cancer worldwide. Recent studies demonstrated that the tumour microenvironment (TME) is pivotal for tumour progression, providing multiple targeting opportunities for therapeutic strategies. As one of the most abundant stromal cell types in the TME, tumour-associated macrophages (TAMs) exhibit high plasticity. Malignant cells alter their metabolic profiles to adapt to the limited availability of oxygen and nutrients in the TME, resulting in functional alteration of TAMs. The metabolic features of TAMs are strongly associated with their functional plasticity, which further impacts metabolic profiling in the TME and contributes to tumourigenesis and progression. Here, we review the functional determination of the TME by TAM metabolic alterations, including glycolysis as well as fatty acid and amino acid metabolism, which in turn are influenced by environmental changes. Additionally, we discuss metabolic reprogramming of TAMs to a tumouricidal phenotype as a potential antitumoural therapeutic strategy.
Effect of Deep Cryogenic Activated Treatment on Hemp Stem-Derived Carbon Used as Anode for Lithium-Ion Batteries
Authors: Li Zhigang , Guan Zhongxiang , Guan Zhiping , Liang Ce , Yu Kaifeng
Published: October 2020

The cryogenic process has been widely applied in various fields, but it has rarely been reported in the preparation of anode materials for lithium-ion battery. In this paper, activated carbon derived from hemp stems was prepared by carbonization and activation; then, it was subjected to cryogenic treatment to obtain cryogenic activated carbon. The characterization results show that the cryogenic activated carbon (CAC) has a richer pore structure than the activated carbon (AC) without cryogenic treatment, and its specific surface area is 1727.96 m2/g. The porous carbon had an excellent reversible capacity of 756.8 mAh/g after 100 cycles at 0.2 C as anode of lithium-ion battery, in which the electrochemical performance of CAC was remarkably improved due to its good pore structure. This provides a new idea for the preparation of anode materials for high-capacity lithium-ion batteries.
Cell cycle control and environmental response by second messengers in Caulobacter crescentus
Authors: Xu Chunrui , Weston Bronson R. , Tyson John J. , Cao Yang
Published: September 2020

Background

Second messengers, c-di-GMP and (p)ppGpp, are vital regulatory molecules in bacteria, influencing cellular processes such as biofilm formation, transcription, virulence, quorum sensing, and proliferation. While c-di-GMP and (p)ppGpp are both synthesized from GTP molecules, they play antagonistic roles in regulating the cell cycle. In C. crescentus, c-di-GMP works as a major regulator of pole morphogenesis and cell development. It inhibits cell motility and promotes S-phase entry by inhibiting the activity of the master regulator, CtrA. Intracellular (p)ppGpp accumulates under starvation, which helps bacteria to survive under stressful conditions through regulating nucleotide levels and halting proliferation. (p)ppGpp responds to nitrogen levels through RelA-SpoT homolog enzymes, detecting glutamine concentration using a nitrogen phosphotransferase system (PTS Ntr). This work relates the guanine nucleotide-based second messenger regulatory network with the bacterial PTS Ntr system and investigates how bacteria respond to nutrient availability.

Results

We propose a mathematical model for the dynamics of c-di-GMP and (p)ppGpp in C. crescentus and analyze how the guanine nucleotide-based second messenger system responds to certain environmental changes communicated through the PTS Ntr system. Our mathematical model consists of seven ODEs describing the dynamics of nucleotides and PTS Ntr enzymes. Our simulations are consistent with experimental observations and suggest, among other predictions, that SpoT can effectively decrease c-di-GMP levels in response to nitrogen starvation just as well as it increases (p)ppGpp levels. Thus, the activity of SpoT (or its homologues in other bacterial species) can likely influence the cell cycle by influencing both c-di-GMP and (p)ppGpp.

Conclusions

In this work, we integrate current knowledge and experimental observations from the literature to formulate a novel mathematical model. We analyze the model and demonstrate how the PTS Ntr system influences (p)ppGpp, c-di-GMP, GMP and GTP concentrations. While this model does not consider all aspects of PTS Ntr signaling, such as cross-talk with the carbon PTS system, here we present our first effort to develop a model of nutrient signaling in C. crescentus.
A stable DNA-free screening system for CRISPR/RNPs-mediated gene editing in hot and sweet cultivars of Capsicum annuum
Authors: Kim Hyeran , Choi Jisun , Won Kang-Hee
Published: October 2020

Background

DNA-free, clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein (Cas) ribonucleoprotein (RNP)-based genome editing is a simple, convincing, and promising tool for precision crop breeding. The efficacy of designed CRISPR-based genome editing tools is a critical prerequisite for successful precision gene editing in crops.

Results

This study demonstrates that soil-grown leaf- or callus-derived pepper protoplasts are a useful system for screening of efficient guide RNAs for CRISPR/Cas9 or CRISPR/Cas12a (Cpf1). CRISPR/Cas9 or Cpf1 were delivered as CRISPR/RNP complexes of purified endonucleases mixed with the designed single guide RNA, which can edit the target gene, CaMLO2 in two pepper cultivars with whole genome sequenced, Capsicum annuum ‘CM334’ and C. annuum ‘Dempsey’. The designed guide RNAs (sgRNAs for Cas9 or crRNAs for Cpf1) are conserved for CaMLO2 in both CM334 and Dempsey and cleave CaMLO2 in vitro. CRISPR/Cas9- or /Cpf1-RNP complexes were transfected into purely isolated protoplasts of the hot pepper CM334 and sweet pepper Dempsey by PEG-mediated delivery. Targeted deep sequencing analysis indicated that the targeted CaMLO2 gene was differentially edited in both cultivars, depending on the applied CRISPR/RNPs.

Conclusions

Pepper protoplast-based CRISPR guide-RNA selection is a robust method to check the efficacy of designed CRISPR tools and is a prerequisite for regenerating edited plants, which is a critical time-limiting procedure. The rapid and convincing selection of guide RNA against a target genome reduces the laborious efforts for tissue culture and facilitates effective gene editing for pepper improvement.
Salt tolerance involved candidate genes in rice: an integrative meta-analysis approach
Authors: Mirdar Mansuri Raheleh , Shobbar Zahra-Sadat , Babaeian Jelodar Nadali , Ghaffari Mohammadreza , Mohammadi Seyed Mahdi , Daryani Parisa
Published: October 2020

Background

Salinity, as one of the main abiotic stresses, critically threatens growth and fertility of main food crops including rice in the world. To get insight into the molecular mechanisms by which tolerant genotypes responds to the salinity stress, we propose an integrative meta-analysis approach to find the key genes involved in salinity tolerance. Herein, a genome-wide meta-analysis, using microarray and RNA-seq data was conducted which resulted in the identification of differentially expressed genes (DEGs) under salinity stress at tolerant rice genotypes. DEGs were then confirmed by meta-QTL analysis and literature review.

Results

A total of 3449 DEGs were detected in 46 meta-QTL positions, among which 1286, 86, 1729 and 348 DEGs were observed in root, shoot, seedling, and leaves tissues, respectively. Moreover, functional annotation of DEGs located in the meta-QTLs suggested some involved biological processes (e.g., ion transport, regulation of transcription, cell wall organization and modification as well as response to stress) and molecular function terms (e.g., transporter activity, transcription factor activity and oxidoreductase activity). Remarkably, 23 potential candidate genes were detected in Saltol and hotspot-regions overlying original QTLs for both yield components and ion homeostasis traits; among which, there were many unreported salinity-responsive genes. Some promising candidate genes were detected such as pectinesterase, peroxidase, transcription regulator, high-affinity potassium transporter, cell wall organization, protein serine/threonine phosphatase, and CBS domain cotaining protein.

Conclusions

The obtained results indicated that, the salt tolerant genotypes use qualified mechanisms particularly in sensing and signalling of the salt stress, regulation of transcription, ionic homeostasis, and Reactive Oxygen Species (ROS) scavenging in response to the salt stress.
Analytical performance evaluation of a commercial next generation sequencing liquid biopsy platform using plasma ctDNA, reference standards, and synthetic serial dilution samples derived from normal plasma
Authors: Verma Suman , Moore Mathew W. , Ringler Rebecca , Ghosal Abhisek , Horvath Kyle , Naef Theodore , Anvari Sheri , Cotter Philip D. , Gunn Shelly
Published: October 2020

Background

Circulating tumor (ct) DNA assays performed in clinical laboratories provide tumor biomarker testing support for biopharmaceutical clinical trials. Yet it is neither practical nor economically feasible for many of these clinical laboratories to internally develop their own liquid biopsy assay. Commercially available ctDNA kits are a potential solution for laboratories seeking to incorporate liquid biopsy into their test menus. However, the scarcity of characterized patient samples and cost of purchasing validation reference standards creates a barrier to entry. In the current study, we evaluated the analytical performance of the AVENIO ctDNA liquid biopsy platform (Roche Sequencing Solutions) for use in our clinical laboratory.

Method

Intra-laboratory performance evaluation of AVENIO ctDNA Targeted, Expanded, and Surveillance kits (Research Use Only) was performed according to College of American Pathologists (CAP) guidelines for the validation of targeted next generation sequencing assays using purchased reference standards, de-identified human plasma cell-free (cf) DNA samples, and contrived samples derived from commercially purchased normal and cancer human plasma. All samples were sequenced at read depths relevant to clinical settings using the NextSeq High Output kit (Illumina).

Results

At the clinically relevant read depth, Avenio ctDNA kits demonstrated 100% sensitivity in detecting single nucleotide variants (SNVs) at ≥0.5% allele frequency (AF) and 50% sensitivity in detecting SNVs at 0.1% AF using 20–40 ng sample input amount. The assay integrated seamlessly into our laboratory’s NGS workflow with input DNA mass, target allele frequency (TAF), multiplexing, and number of reads optimized to support a high-throughput assay appropriate for biopharmaceutical trials.

Conclusions

Our study demonstrates that AVENIO ctDNA liquid biopsy platform provides a viable alternative for efficient incorporation of liquid biopsy assays into the clinical laboratory for detecting somatic alterations as low as 0.5%. Accurate detection of variants lower than 0.5% could potentially be achieved by deeper sequencing when clinically indicated and economically feasible.
The FeverApp registry – ecological momentary assessment (EMA) of fever management in families regarding conformity to up-to-date recommendations
Authors: Martin David , Wachtmeister Jana , Ludwigs Kai , Jenetzky Ekkehart
Published: October 2020

Background

Fever is one of the most common symptoms of pediatric consultations and its mismanagement is a health care burden. Guidelines on fever management are incoherent and data on fever management are still missing. This study protocol describes an app-based registry to evaluate the fever management of parents.

Objective

The primary objectives are to assess guideline adherence (primary outcome) and parental confidence in managing fever, and thus to reduce overuse of antipyretics, antibiotics and healthcare providers.

Secondary objectives include creating a “FeverApp” that will enable parents to handle fever safely and to use the FeverApp registry as symptom and fever management diary. Further objectives include developing and testing a symptom-led registry model by app-based acquisition of parental entries of febrile illness cycle data and developing and testing models of how an interactive app-based registry can enable nationwide EMA information to inform science, guideline and policy makers, and the public.

Methods

A FeverApp, guiding parents and carers in handling and documenting fever, will be developed with family pediatricians according to current guidelines and recommended for all parents in Germany. A registry will anonymously document features, management and outcomes of febrile episodes: basic sociodemographic and medical information, initial symptoms, course of fever, pharmacological and non-pharmacological interventions, consultations with doctors, outcomes, fever-associated fears, and app satisfaction.

Results

This app may improve communication quality and health, e.g. asthma and antimicrobial resistance. Results will be published via website www.feverapp.de.

Trial registration

This app-based registry protocol is registered in the German Clinical Trials Register (DRKS) with registration number: DRKS00016591.