bluuebunny/crossref_metadata_2025 huggingface dataset:
Molecular Mechanisms of Succinimide Formation from Aspartic Acid Residues Catalyzed by Two Water Molecules in the Aqueous Phase
Authors: Nakayoshi Tomoki , Kato Koichi , Fukuyoshi Shuichi , Takahashi Ohgi , Kurimoto Eiji , Oda Akifumi
Published: January 2021

Aspartic acid (Asp) residues are prone to nonenzymatic isomerization via a succinimide (Suc) intermediate. The formation of isomerized Asp residues is considered to be associated with various age-related diseases, such as cataracts and Alzheimer’s disease. In the present paper, we describe the reaction pathway of Suc residue formation from Asp residues catalyzed by two water molecules using the B3LYP/6-31+G(d,p) level of theory. Single-point energies were calculated using the MP2/6-311+G(d,p) level of theory. For these calculations, we used a model compound in which an Asp residue was capped with acetyl and methylamino groups on the N- and C-termini, respectively. In the aqueous phase, Suc residue formation from an Asp residue was roughly divided into three steps, namely, iminolization, cyclization, and dehydration, with the activation energy estimated to be 109 kJ mol−1. Some optimized geometries and reaction modes in the aqueous phase were observed that differed from those in the gas phase.
Ingested Ketone Ester Leads to a Rapid Rise of Acetyl-CoA and Competes with Glucose Metabolism in the Brain of Non-Fasted Mice
Authors: Suissa Laurent , Kotchetkov Pavel , Guigonis Jean-Marie , Doche Emilie , Osman Ophélie , Pourcher Thierry , Lindenthal Sabine
Published: January 2021

The role of ketone bodies in the cerebral energy homeostasis of neurological diseases has begun to attract recent attention particularly in acute neurological diseases. In ketogenic therapies, ketosis is achieved by either a ketogenic diet or by the administration of exogenous ketone bodies. The oral ingestion of the ketone ester (KE), (R)-3-hydroxybutyl (R)-3-hydroxybutyrate, is a new method to generate rapid and significant ketosis (i.e., above 6 mmol/L) in humans. KE is hydrolyzed into β-hydroxybutyrate (βHB) and its precursor 1,3-butanediol. Here, we investigate the effect of oral KE administration (3 mg KE/g of body weight) on brain metabolism of non-fasted mice using liquid chromatography in tandem with mass spectrometry. Ketosis (Cmax = 6.83 ± 0.19 mmol/L) was obtained at Tmax = 30 min after oral KE-gavage. We found that βHB uptake into the brain strongly correlated with the plasma βHB concentration and was preferentially distributed in the neocortex. We showed for the first time that oral KE led to an increase of acetyl-CoA and citric cycle intermediates in the brain of non-fasted mice. Furthermore, we found that the increased level of acetyl-CoA inhibited glycolysis by a feedback mechanism and thus competed with glucose under physiological conditions. The brain pharmacodynamics of this oral KE strongly suggest that this agent should be considered for acute neurological diseases.
Two-Dimensional Visualization of the Three-Dimensional Planned Sacroiliac Screw Corridor with the Slice Fusion Method
Authors: Kerschbaum Maximilian , Lang Siegmund , Baumann Florian , Alt Volker , Worlicek Michael
Published: January 2021

Insertion of sacro-iliac (SI) screws for stabilization of the posterior pelvic ring without intraoperative navigation or three-dimensional imaging can be challenging. The aim of this study was to develop a simple method to visualize the ideal SI screw corridor, on lateral two-dimensional images, corresponding to the lateral fluoroscopic view, used intraoperatively while screw insertion, to prevent neurovascular injury. We used multiplanar reconstructions of pre- and postoperative computed tomography scans (CT) to determine the position of the SI corridor. Then, we processed the dataset into a lateral two-dimensional slice fusion image (SFI) matching head and tip of the screw. Comparison of the preoperative SFI planning and the screw position in the postoperative SFI showed reproducible results. In conclusion, the slice fusion method is a simple technique for translation of three-dimensional planned SI screw positioning into a two-dimensional strict lateral fluoroscopic-like view.
Effective Orifice Area of Balloon-Expandable and Self-Expandable Transcatheter Aortic Valve Prostheses: An Echo Doppler Comparative Study
Authors: Kanso Mohamad , Kibler Marion , Hess Sebastien , Rischner Jérome , Plastaras Philoktimon , Kindo Michel , Hoang Minh , De Poli Fabien , Leddet Pierre , Petit Hélène , Zeyons Floriane , Trinh Annie , Matsushita Kensuke , Morel Olivier , Ohlmann Patrick
Published: January 2021

Published data on the size-specific effective orifice area (EOA) of transcatheter heart valves (THVs) remain scarce. Here, we sought to investigate the intra-individual changes in EOA and mean transvalvular aortic gradient (MG) of the Sapien 3 (S3), CoreValve (CV), and Evolut R (EVR) prostheses both at short-term and at 1-year follow-up. The study sample consisted of 260 consecutive patients with severe aortic stenosis who underwent transcatheter aortic valve implantation (TAVI). EOAs and MGs were measured with Doppler echocardiography for the following prostheses: S3 23 mm (n = 74; 28.5%), S3 26 mm (n = 67; 25.8%), S3 29 mm (n = 20; 7.7%), CV 23 mm (n = 2; 0.8%), CV 26 mm (n = 15; 5.8%), CV 29 mm (n = 24; 9.2%), CV 31 mm (n = 9; 3.5%), EVR 26 mm (n = 22; 8.5%), and EVR 29 mm (n = 27; 10.4%). Values were obtained at discharge, 1 month, 6 months, and 1 year from implantation. At discharge, EOAs were larger and MGs lower for larger-size prostheses, regardless of being balloon-expandable or self-expandable. In patients with small aortic annulus size, the hemodynamic performances of CV and EVR prostheses were superior to those of S3. However, we did not observe significant differences in terms of all-cause mortality according to THV type or size. Both balloon-expandable and self-expandable new-generation THVs show excellent hemodynamic performances without evidence of very early valve degeneration.
Associations of Body Composition, Maximum Strength, Power Characteristics with Sprinting, Jumping, and Intermittent Endurance Performance in Male Intercollegiate Soccer Players
Authors: Ishida Ai , Travis S. , Stone Michael
Published: January 2021

The purpose of this study was to determine the relationships between body composition, strength, power characteristics, sprinting, jumping, and intermittent endurance performance in collegiate male players. Twenty-three players participated (19.7 ± 1.6 yrs; 71.8 ± 7.1 kg; 176.5 ± 5.1 cm). Measurements of interest in body composition included body fat percentage (BF%), lean body mass (LBM), and body mass (BM). Power characteristics were measured with an unloaded squat jump (SJ0) and loaded SJ at 20 kg (SJ20) and 40 kg (SJ40), and unloaded countermovement jump (CMJ0). Power assessments included peak power (PP) and PP allometrically scaled (PPa). Strength characteristics were assessed using isometric mid-thigh pull. Strength assessment included isometric peak force (IPF) and IPF allometrically scaled (IPFa). Performance measures included 10m and 20 m sprint time, CMJ0 jump-height, and Yo-Yo intermittent endurance test level 1 distance. Significant correlations ranging from moderate to very large were found for LBM and CMJ jump height (CM0 JH) (p = 0.01, r = 0.50); BF% and sprint times at 10 m (p = 0.03, r = 0.44) and 20 m (p = 0.02, r = 0.50). PP and PPa from SJ0 and CMJ0 were significantly correlated to 10m sprint time (p < 0.05, r = −0.45 to −0.53) and 20 m sprint time (p < 0.05, r = −0.40 to −0.49). Our findings agree with previous literature in that body composition and power characteristics are directly related to soccer-related performance.
Aspergillus fumigatus, One Uninucleate Species with Disparate Offspring
Authors: Danion François , van Rhijn Norman , Dufour Alexandre C. , Legendre Rachel , Sismeiro Odile , Varet Hugo , Olivo-Marin Jean-Christophe , Mouyna Isabelle , Chamilos Georgios , Bromley Michael , Beauvais Anne , Latgé Jean-Paul
Published: January 2021

Establishment of a fungal infection due to Aspergillus fumigatus relies on the efficient germination of the airborne conidia once they penetrate the respiratory tract. However, the features of conidial germination have been poorly explored and understood in this fungal species as well as in other species of filamentous fungi. We show here that the germination of A. fumigatus is asynchronous. If the nutritional environment and extensive gene deletions can modify the germination parameters for A. fumigatus, the asynchrony is maintained in all germinative conditions tested. Even though the causes for this asynchrony of conidial germination remain unknown, asynchrony is essential for the completion of the biological cycle of this filamentous fungus.
Mechanotransduction in Prokaryotes: A Possible Mechanism of Spaceflight Adaptation
Authors: Fajardo-Cavazos Patricia , Nicholson Wayne L.
Published: January 2021

Our understanding of the mechanisms of microgravity perception and response in prokaryotes (Bacteria and Archaea) lag behind those which have been elucidated in eukaryotic organisms. In this hypothesis paper, we: (i) review how eukaryotic cells sense and respond to microgravity using various pathways responsive to unloading of mechanical stress; (ii) we observe that prokaryotic cells possess many structures analogous to mechanosensitive structures in eukaryotes; (iii) we review current evidence indicating that prokaryotes also possess active mechanosensing and mechanotransduction mechanisms; and (iv) we propose a complete mechanotransduction model including mechanisms by which mechanical signals may be transduced to the gene expression apparatus through alterations in bacterial nucleoid architecture, DNA supercoiling, and epigenetic pathways.
Raising Microbiology Research to the Next Level
Authors: Cenci-Goga Beniamino
Published: January 2021

It is my great pleasure to introduce Microbiology Research under the new publisher, MDPI [...]
Optimizing a Suspension Culture Method with a Decreased Cost to Detect Enteroviruses in Water to Increase Surveillance Access
Authors: Tornberg-Belanger Stephanie , Sreter Jonathan , Margolin Aaron
Published: January 2021

Enteroviruses are a public health threat due to the high incidence of infections and potential for serious illness or death. Some laboratories in high-income countries detect enteroviruses in water by integrating cell culture and PCR (ICC/PCR). This combined method carries a high financial burden, due in part to specialized cell culture equipment. Therefore, we expanded upon a pilot study to reduce the cost by using common laboratory polypropylene tubes to create a cell culture in suspension. We optimized the protocol by determining minimal incubation periods post-infection as a function of the initial virus concentration. Cells in suspension and traditional monolayers were inoculated with poliovirus and incubated in 8-hour intervals up to 48 hours prior to extraction. Quantitative PCR (qPCR) was used to detect viral nucleic acid targets. Treated and raw water samples were seeded with virus and the suspension ICC/qPCR protocol used to ascertain whether the protocol performed similar to directly seeding cells. No variation in virus detection occurred using the suspension ICC/qPCR or monolayer ICC/qPCR (p = 0.95). In surface water samples, viral nucleic acid was successfully detected, with no significant increase after 32 h (p > 0.05). Suspension ICC/qPCR is as effective as monolayer ICC/qPCR in detecting enteroviruses in surface waters. Materials used in the suspension ICC/qPCR have a lower monetary cost than traditional cell culture materials without loss of sensitivity. More accessible testing of waters for enterovirus contamination through cost reduction has the potential to reduce human exposure and disease.
New Trends in Meat Packaging
Authors: Cenci-Goga Beniamino , Iulietto Maria , Sechi Paola , Borgogni Elena , Karama Musafiri , Grispoldi Luca
Published: January 2021

The term ‘packaging’ refers to the technological intervention aimed at the protection of food from a variety of factors, which provokes the product detriment. Packaging is considered as one of the most interesting technological aspects and a constantly evolving issue in food production. This paper aims at the evaluation of the properties of packaging currently used in the meat industry and analyses the advantages, the disadvantages and the microbiota involved. Packaging is a coordinated system, which prepares the products for transportation, distribution, storage, marketing and consumption. Even if several packaging alternatives are proposed, the common purpose is to guarantee high standards, yet maintaining the required characteristics as long as possible. Meat is a dynamic system with a limited shelf-life and the nutritional and sensory properties may change during storage due to microbial activity and physical or chemical changes. Microbial spoilage, for instance, determines an impact in meat, producing unattractive odours, flavours, discolouration, gas and slime.
Influence of the Degree of Deacetylation of Chitosan and BMP-2 Concentration on Biocompatibility and Osteogenic Properties of BMP-2/PLA Granule-Loaded Chitosan/β-Glycerophosphate Hydrogels
Authors: Vasilyev Andrey Vyacheslavovich , Kuznetsova Valeriya Sergeevna , Bukharova Tatyana Borisovna , Grigoriev Timofei Evgenevich , Zagoskin Yuriy Dmitrievich , Nedorubova Irina Alekseevna , Babichenko Igor Ivanovich , Chvalun Sergey Nicolaevich , Goldstein Dmitry Vadimovich , Kulakov Anatoliy Alekseevich
Published: January 2021

Compositions based on chitosan/β-glycerophosphate hydrogels with highly porous polylactide granules can be used to obtain moldable bone graft materials that have osteoinductive and osteoconductive properties. To eliminate the influence of such characteristics as chain length, degree of purification, and molecular weight on a designed material, the one-stock chitosan sample was reacetylated to degrees of deacetylation (DD%) of 19.5, 39, 49, 55, and 56. A study of the chitosan/β-glycerophosphate hydrogel with chitosan of a reduced DD% showed that a low degree of deacetylation increased the MSCs (multipotent stromal cells) viability rate in vitro and reduced the leukocyte infiltration in subcutaneous implantation to Wistar rats in vivo. The addition of 12 wt% polylactide granules resulted in optimal composite mechanical and moldable properties, and increased the modulus of elasticity of the hydrogel-based material by approximately 100 times. Excessive filling of the material with PLA (polylactide) granules (more than 20%) led to material destruction at a ~10% strain. Osteoinductive and osteoconductive properties of the chitosan hydrogel-based material with reacetylated chitosan (39 DD%) and highly porous polylactide granules impregnated with BMP-2 (bone morphogenetic protein-2) have been demonstrated in models of orthotopic and ectopic bone formation. When implanted into a critical-size calvarial defect in rats, the optimal concentration of BMP-2 was 10 μg/mL: bone tissue areas filled the entire material’s thickness. Implantation of the material with 50 μg/mL BMP-2 was accompanied with excessive growth of bone tissue and material displacement beyond the defect. Significant osteoinductive and osteoconductive properties of the material with 10 μg/mL of BMP-2 were also shown in subcutaneous implantation.
Volumetric Absorptive Microsampling of Blood for Untargeted Lipidomics
Authors: Marasca Camilla , Arana Maria Encarnacion Blanco , Protti Michele , Cavalli Andrea , Mercolini Laura , Armirotti Andrea
Published: January 2021

In the present, proof-of-concept paper, we explore the potential of one common solid support for blood microsampling (dried blood spot, DBS) and a device (volumetric absorptive microsampling, VAMS) developed for the untargeted lipidomic profiling of human whole blood, performed by high-resolution LC-MS/MS. Dried blood microsamples obtained by means of DBS and VAMS were extracted with different solvent compositions and compared with fluid blood to evaluate their efficiency in profiling the lipid chemical space in the most broad way. Although more effort is needed to better characterize this approach, our results indicate that VAMS is a viable option for untargeted studies and its use will bring all the corresponding known advantages in the field of lipidomics, such as haematocrit independence.
Oral Vitamin D Therapy in Patients with Psoriasis
Authors: Stanescu Ana Maria Alexandra , Simionescu Anca Angela , Diaconu Camelia Cristina
Published: January 2021

Vitamin D treatment is effective when applied topically to the skin for plaque-type psoriasis. Oral vitamin D supplementation might be effective as an adjuvant treatment option in psoriasis. This umbrella review aimed to highlight the current knowledge regarding the use of oral vitamin D for treatment of patients with psoriasis. We performed a literature search and identified 107 eligible full-text articles that were relevant to the research interest. Among these, 10 review articles were selected, and data were extracted. A data synthesis showed that only a few studies monitored oral vitamin D efficacy in patients with psoriasis. No studies investigated the optimal dose of systemic vitamin D in psoriasis. However, most studies did not observe side effects for doses within a relatively narrow range (0.25 to 2 μg/day). These results suggest that more large-scale studies are needed to determine the efficacy, optimal dose, and adverse effects of vitamin D administration in patients with psoriasis.
Body Composition Changes after a Weight Loss Intervention: A 3-Year Follow-Up Study
Authors: Rojo-Tirado Miguel A. , Benito Pedro J. , Ruiz Jonatan R. , Ortega Francisco B. , Romero-Moraleda Blanca , Butragueño Javier , Bermejo Laura M. , Castro Eliane A. , Gómez-Candela Carmen
Published: January 2021

Studies comparing different types of exercise-based interventions have not shown a consistent effect of training on long-term weight maintenance. The aim of this study was to compare the effects of exercise modalities combined with diet intervention on body composition immediately after intervention and at 3 years’ follow-up in overweight and obese adults. Two-hundred thirty-nine people (107 men) participated in a 6-month diet and exercise-based intervention, split into four randomly assigned groups: strength group (S), endurance group (E), combined strength and endurance group (SE), and control group (C). The body composition measurements took place on the first week before the start of training and after 22 weeks of training. In addition, a third measurement took place 3 years after the intervention period. A significant interaction effect (group × time) (p = 0.017) was observed for the fat mass percentage. It significantly decreased by 5.48 ± 0.65%, 5.30 ± 0.65%, 7.04 ± 0.72%, and 4.86 ± 0.65% at post-intervention for S, E, SE, and C, respectively. Three years after the intervention, the fat mass percentage returned to values similar to the baseline, except for the combined strength and endurance group, where it remained lower than the value at pre-intervention (p < 0.05). However, no significant interaction was discovered for the rest of the studied outcomes, neither at post-intervention nor 3 years later. The combined strength and endurance group was the only group that achieved lower levels of fat mass (%) at both post-intervention and 3 years after intervention, in comparison with the other groups.
Drug Conjugated and Bispecific Antibodies for Multiple Myeloma: Improving Immunotherapies off the Shelf
Authors: Barilà Gregorio , Rizzi Rita , Zambello Renato , Musto Pellegrino
Published: January 2021

The impressive improvement of overall survival in multiple myeloma (MM) patients in the last years has been mostly related to the availability of new classes of drugs with different mechanisms of action, including proteasome inhibitors (PI), immunomodulating agents (IMiDs), and monoclonal antibodies. However, even with this increased potence of fire, MM still remains an incurable condition, due to clonal selection and evolution of neoplastic clone. This concept underlines the importance of immunotherapy as one of the most relevant tools to try to eradicate the disease. In line with this concept, active and passive immunotherapies represent the most attractive approach to this aim. Antibody-drug conjugate(s) (ADCs) and bispecific antibodies (BsAbs) include two innovative tools in order to limit neoplastic plasma cell growth or even, if used at the time of the best response, to potentially eradicate the tumoral clone. Following their promising results as single agent for advanced disease, at the recent 62nd ASH meeting, encouraging data of several combinations, particularly of ADC(s) with PI or IMiDs, have been reported, suggesting even better results for patients treated earlier. In this paper, we reviewed the characteristics, mechanism of action, and clinical data available for most relevant ADC(s) and BsAbs.
CPE-DB: An Open Database of Chemical Penetration Enhancers
Authors: Vasyuchenko Ekaterina P. , Orekhov Philipp S. , Armeev Grigoriy A. , Bozdaganyan Marine E.
Published: January 2021

The cutaneous delivery route currently accounts for almost 10% of all administered drugs and it is becoming more common. Chemical penetration enhancers (CPEs) increase the transport of drugs across skin layers by different mechanisms that depend on the chemical nature of the penetration enhancers. In our work, we created a chemical penetration enhancer database (CPE-DB) that is, to the best of our knowledge, the first CPE database. We collected information about known enhancers and their derivatives in a single database, and classified and characterized their molecular diversity in terms of scaffold content, key chemical moieties, molecular descriptors, etc. CPE-DB can be used for virtual screening and similarity search to identify new potent and safe enhancers, building quantitative structure–activity relationship (QSAR) and quantitative structure–property relationship (QSPR) models, and other machine-learning (ML) applications for the prediction of biological activity.
Study of the Influence of PCL on the In Vitro Degradation of Extruded PLA Monofilaments and Melt-Spun Filaments
Authors: Barral Vivien , Dropsit Sophie , Cayla Aurélie , Campagne Christine , Devaux Éric
Published: January 2021

This work presents the effect of a melt-spinning process on the degradation behavior of bioresorbable and immiscible poly(d,l-lactide) (PLA) and polycaprolactone (PCL) polymer blends. A large range of these blends, from PLA90PCL10 (90 wt% PLA and 10 wt% PCL) to PLA60PCL40 in increments of 10%, was processed via extrusion (diameter monofilament: ∅ ≈ 1 mm) and melt spinning (80 filaments: 50 to 70 µm each) to evaluate the impact of the PCL ratio and then melt spinning on the hydrolytic degradation of PLA, which allowed for highlighting the potential of a textile-based scaffold in bioresorbable implants. The morphologies of the structures were investigated via extracting PCL with acetic acid and scanning electron microscopy observations. Then, they were immersed in a Dulbecco’s Modified Eagle Medium (DMEM) media at 50 °C for 35 days and their properties were tested in order to evaluate the relation between the morphology and the evolution of the crystallinity degree and the mechanical and physical properties. As expected, the incorporation of PCL into the PLA matrix slowed down the hydrolytic degradation. It was shown that the degradation became heterogeneous with a small ratio of PCL. Finally, melt spinning had an impact on the morphology, and consequently, on the other properties over time.
Mechanical Properties of Protective Coatings against Marine Fouling: A Review
Authors: Pistone Alessandro , Scolaro Cristina , Visco Annamaria
Published: January 2021

The accumulation of marine organisms on ship hulls, such as microorganisms, barnacles, and seaweeds, represents a global problem for maritime industries, with both economic and environmental costs. The use of biocide-containing paints poses a serious threat to marine ecosystems, affecting both target and non-target organisms driving science and technology towards non-biocidal solutions based on physico-chemical and materials properties of coatings. The review reports recent development of hydrophobic protective coatings in terms of mechanical properties, correlated with the wet ability features. The attention is focused mainly on coatings based on siloxane and epoxy resin due to the wide application fields of such systems in the marine industry. Polyurethane and other systems have been considered as well. These coatings for anti-fouling applications needs to be both long-term mechanically stable, perfectly adherent with the metallic/composite substrate, and capable to detach/destroy the fouling organism. Prospects should focus on developing even “greener” antifouling coatings solutions. These coatings should also be readily addressable to industrial scale-up for large-scale product distribution, possibly at a reasonable cost.
Electrospun PVDF Nanofibers for Piezoelectric Applications: A Review of the Influence of Electrospinning Parameters on the β Phase and Crystallinity Enhancement
Authors: He Zhongchen , Rault François , Lewandowski Maryline , Mohsenzadeh Elham , Salaün Fabien
Published: January 2021

Polyvinylidene fluoride (PVDF) is among the most attractive piezo-polymers due to its excellent piezoelectricity, lightweight, flexibility, high thermal stability, and chemical resistance. PVDF can exist under different forms of films, membranes, and (nano)fibers, and its piezoelectric property related to its β phase content makes it interesting for energy harvesters and wearable applications. Research investigation shows that PVDF in the form of nanofibers prepared by electrospinning has more flexibility and better air permeability, which make them more suitable for these types of applications. Electrospinning is an efficient technique that produces PVDF nanofibers with a high β phase fraction and crystallinity by aligning molecular dipoles (–CH2 and –CF2) along an applied voltage direction. Different nanofibers production techniques and more precisely the electrospinning method for producing PVDF nanofibers with optimal electrospinning parameters are the key focuses of this paper. This review article highlights recent studies to summarize the influence of electrospinning parameters such as process (voltage, distance, flow rate, and collector), solution (Mw, concentration, and solvent), and ambient (humidity and temperature) parameters to enhance the piezoelectric properties of PVDF nanofibers. In addition, recent development regarding the effect of adding nanoparticles in the structure of nanofibers on the improvement of the β phase is reviewed. Finally, different methods of measuring piezoelectric properties of PVDF nanofibrous membrane are discussed.
Chitosan Plasma Chemical Processing in Beam-Plasma Reactors as a Way of Environmentally Friendly Phytostimulants Production
Authors: Vasilieva Tatiana , Goñi Oscar , Quille Patrick , O’Connell Shane , Kosyakov Dmitry , Shestakov Semen , Ul’yanovskii Nikolay , Vasiliev Michael
Published: January 2021

A novel technique of phytoactive water-soluble chitooligosaccharide (COS) production in low-temperature plasma is described. Design, operation, and control of plasma chemical reactors used to produce COS from the powder of high molecular weight chitosan are presented. The electron beam plasma is strongly non-equilibrium and chemically active; plasma was excited by injecting the scanning electron beam into reaction volume filled with aerosol, containing oxygen and chitosan powder. Plasma chemical processes, responsible for the raw chitosan destruction and techniques of these processes to obtain control of products of optimal molecular weight, are considered. COS, in amounts sufficient for laboratory tests with some plants, were produced. Tests showed that the addition of COS into the liquid growing medium at 0.25 and 1 mg/mL stimulates root growth in Arabidopsis thaliana seedlings (Col-0) by up to 40%, with respect to control plants. Foliar application of these COS formulations at 0.25 mg/mL on tomato plants (cv. Micro-Tom) also resulted in increases between 11.9% and 36% in two important plant productivity indicators (flower and fruit numbers) compared to the control plants. Being environmentally friendly (and resource saving) the electron beam plasma technology of renewable natural biopolymer processing can be considered as a competitive way to produce biostimulants for commercial agriculture.
Separation of Church and State, American Exceptionalism, and the Contemporary Social Moment: Viewing Church–State Separation from the Priority of Slavery
Authors: Prud'homme Joseph
Published: January 2021

The contemporary social moment in the United States has affirmed the critical importance of racial justice, and especially claims to justice informed by the contributions of structural and institutional forces connected with the nation’s original sin of slavery. In this paper, I examine the contributions of strict church–state separationism to the maintenance of slavery in the antebellum South in comparison to the contributions various forms of religious establishment made to the successful abolition of slavery in the United Kingdom and the British Empire. Developing a deeper historical understanding of the ways the relationship between religious and governmental institutions influenced the abolition and maintenance of slavery can assist the contemporary quest for racial justice.
Non-Linear Modeling of Detectability of Ship Wake Components in Dependency to Influencing Parameters Using Spaceborne X-Band SAR
Authors: Tings Björn
Published: January 2021

The detection of the wakes of moving ships in Synthetic Aperture Radar (SAR) imagery requires the presence of wake signatures, which are sufficiently distinctive from the ocean background. Various wake components exist, which constitute the SAR signatures of ship wakes. For successful wake detection, the contrast between the detectable wake components and the background is crucial. The detectability of those wake components is affected by a number of parameters, which represent the image acquisition settings, environmental conditions or ship properties including voyage information. In this study the dependency of the detectability of individual wake components to these parameters is characterized. For each wake component a detectability model is built, which takes the influence of incidence angle, polarization, wind speed, wind direction, sea state (significant wave height, wavelength, wave direction), vessel’s velocity, vessel’s course over ground and vessel’s length into account. The presented detectability models are based on regression or classification using Support Vector Machines and a dataset of manually labelled TerraSAR‑X wake samples. The considered wake components are: near‑hull turbulences, turbulent wakes, Kelvin wake arms, Kelvin wake’s transverse waves, Kelvin wake’s divergent waves, V‑narrow wakes and ship‑generated internal waves. The statements derived about wake component detectability are mainly in good agreement with statements from previous research, but also some new assumptions are provided. The most expressive influencing parameter is the movement velocity of the vessels, as all wake components are more detectable the faster vessels move.
Vegetation Abundance and Health Mapping Over Southwestern Antarctica Based on WorldView-2 Data and a Modified Spectral Mixture Analysis
Authors: Sun Xiaohui , Wu Wenjin , Li Xinwu , Xu Xiyan , Li Jinfeng
Published: January 2021

In polar regions, vegetation is especially sensitive to climate dynamics and thus can be used as an indicator of the global and regional environmental change. However, in Antarctica, there is very little information on vegetation distribution and growth status. To fill this gap, we evaluated the ability of both linear and nonlinear spectral mixture analysis (SMA) models, including a group of newly developed modified Nascimento’s models for Antarctic vegetated areas (MNM-AVs), in estimating the abundance of major Antarctic vegetation types, i.e., mosses and lichens. The study was conducted using WorldView-2 satellite data and field measurements over the Fildes Peninsula and its surroundings, which are representative vegetated areas in Antarctica. In MNM-AVs, we introduced secondary scattering components for vegetation and its background to account for the sparsity of vegetation cover and reassigned their coefficients. The new models achieved improved performances, among which MNM-AV3 achieved the lowest error for mosses (lichens) abundance estimation with RMSE = 0.202 (0.213). Compared with MNM-AVs, the linear model performed particularly poor for lichens (RMSE = 0.322), which is in contrast to the case of mosses (RMSE = 0.212), demonstrating that spectral signals of lichens are more prone to mix with their backgrounds. Abundance maps of mosses and lichens, as well as a map of moss health status for the entire study area, were then obtained based on MNM-AV3 with around 80% overall accuracy. Moss areas account for 0.7695 km2 in Fildes and 0.3259 km2 in Ardley Island; unhealthy mosses amounted to 40% (49%) of the area in the summer of 2018 (2019), indicating considerable environmental stress.
Detailed Characterization and Monitoring of a Retrogressive Thaw Slump from Remotely Piloted Aircraft Systems and Identifying Associated Influence on Carbon and Nitrogen Export
Authors: Turner Kevin W. , Pearce Michelle D. , Hughes Daniel D.
Published: January 2021

Ice-rich permafrost landscapes are sensitive to ongoing changes in climate. Permafrost retrogressive thaw slumps (RTSs) represent one of the more abrupt and prolonged disturbances, which occur along Arctic river and lake shorelines. These features impact local travel and infrastructure, and there are many questions regarding associated impacts on biogeochemical cycling. Predicting the duration and magnitude of impacts requires that we enhance our knowledge of RTS geomorphological drivers and rates of change. Here we demonstrate the utility of remotely piloted aircraft systems (RPAS) for documenting the volumetric change, associated drivers and potential impacts of the largest active RTS along the Old Crow River in Old Crow Flats, Yukon, Canada. RPAS surveys revealed that 29,174 m3 of sediment was exported during the initial evacuation in June 2016 and an additional 18,845 m3 continued to be exported until June 2019. More sediment export occurred during the warmer 2017 summer that experienced less cumulative rainfall than summer 2018. However, several rain events during 2017 were of higher intensity than during 2018. Overall mean soil organic carbon (SOC) and total nitrogen (TN) within sampled thaw slump sediment was 1.36% and 0.11%, respectively. A combination of multispectral, thermal and irradiance (derived from the RPAS digital surface model) data provided detailed classification of thaw slump floor terrain types including raised dry clay lobes, shaded and relatively stable, and low-lying evacuation-prone sediments. Notably, the path of evacuation-prone sediments extended to a series of ice wedges in the northern headwall, where total irradiance was highest. Using thaw slump floor mean SOC and TN values in conjunction with sediment bulk density and thaw slump fill volume, we estimated that 713 t SOC and 58 t TN were exported to the Old Crow River during the three-year study. Findings showcase the utility of high-resolution RPAS datasets for refining our knowledge of thaw slump geomorphology and associated impacts.
Geomorphological and Climatic Drivers of Thermokarst Lake Area Increase Trend (1999–2018) in the Kolyma Lowland Yedoma Region, North-Eastern Siberia
Authors: Veremeeva Alexandra , Nitze Ingmar , Günther Frank , Grosse Guido , Rivkina Elizaveta
Published: January 2021

Thermokarst lakes are widespread in Arctic lowlands. Under a warming climate, landscapes with highly ice-rich Yedoma Ice Complex (IC) deposits are particularly vulnerable, and thermokarst lake area dynamics serve as an indicator for their response to climate change. We conducted lake change trend analysis for a 44,500 km2 region of the Kolyma Lowland using Landsat imagery in conjunction with TanDEM-X digital elevation model and Quaternary Geology map data. We delineated yedoma–alas relief types with different yedoma fractions, serving as a base for geospatial analysis of lake area dynamics. We quantified lake changes over the 1999–2018 period using machine-learning-based classification of robust trends of multi-spectral indices of Landsat data and object-based long-term lake detection. We analyzed the lake area dynamics separately for 1999–2013 and 1999–2018 periods, including the most recent five years that were characterized by very high precipitation. Comparison of drained lake basin area with thermokarst lake extents reveal the overall limnicity decrease by 80% during the Holocene. Current climate warming and wetting in the region led to a lake area increase by 0.89% for the 1999–2013 period and an increase by 4.15% for the 1999–2018 period. We analyzed geomorphological factors impacting modern lake area changes for both periods such as lake size, elevation, and yedoma–alas relief type. We detected a lake area expansion trend in high yedoma fraction areas indicating ongoing Yedoma IC degradation by lake thermokarst. Our concept of differentiating yedoma–alas relief types helps to characterize landscape-scale lake area changes and could potentially be applied for refined assessments of greenhouse gas emissions in Yedoma regions. Comprehensive geomorphological inventories of Yedoma regions using geospatial data provide a better understanding of the extent of thermokarst processes during the Holocene and the pre-conditioning of modern thermokarst lake area dynamics.
Investigating Ground Subsidence and the Causes over the Whole Jiangsu Province, China Using Sentinel-1 SAR Data
Authors: Zhang Yonghong , Wu Hongan , Li Mingju , Kang Yonghui , Lu Zhong
Published: January 2021

Interferometric synthetic aperture radar (InSAR) mapping of localized ground surface deformation has become an important tool to manage subsidence-related geohazards. However, monitoring land surface deformation using InSAR at high spatial resolution over a large region is still a formidable task. In this paper, we report a research on investigating ground subsidence and the causes over the entire 107, 200 km2 province of Jiangsu, China, using time-series InSAR. The Sentinel-1 Interferometric Wide-swath (IW) images of 6 frames are used to map ground subsidence over the whole province for the period 2016–2018. We present processing methodology in detail, with emphasis on the three-level co-registration scheme of S-1 data, retrieval of mean subsidence velocity (MSV) and subsidence time series, and mosaicking of multiple frames of results. The MSV and subsidence time series are generated for 9,276,214 selected coherent pixels (CPs) over the Jiangsu territory. Using 688 leveling measurements in evaluation, the derived MSV map of Jiangsu province shows an accuracy of 3.9 mm/year. Moreover, subsidence causes of the province are analyzed based on InSAR-derived subsidence characteristics, historical optical images, and field-work findings. Main factors accounting for the observed subsidence include: underground mining, groundwater withdrawal, soil consolidations of marine reclamation, and land-use transition from agricultural (paddy) to industrial land. This research demonstrates not only the capability of S-1 data in mapping ground deformation over wide areas in coastal and heavily vegetated region of China, but also the potential of inferring valuable knowledge from InSAR-derived results.
Experimental Study on Dispersion Effects of F (1,1) Wave Mode on Thin Waveguide When Embedded with Fluid
Authors: Raja Nishanth , Balasubramaniam Krishnan
Published: January 2021

This paper reports the simultaneous generation of multiple fundamental ultrasonic guided wave modes L(0,1), T(0,1), and F(1,1) on a thin wire-like waveguide (SS-308L) and its interactions with liquid loading in different attenuation dispersion regimes. An application towards liquid level measurements using these dispersion effects was also demonstrated. The finite element method (FEM) was used to understand the mode behavior and their dispersion effects at different operating frequencies and subsequently validated with experiments. In addition, the ideal configuration for the simultaneous generation of at least two modes (L(0,1), T(0,1), or F(1,1)) is reported. These modes were transmitted/received simultaneously on the waveguide by an ultrasonic shear wave transducer aligned at 0°/45°/90° to the waveguide axis. Level measurement experiments were performed in deionized water and the flexural mode F(1,1) was observed to have distinct dispersion effects at various frequency ranges (i.e., >250 kHz, >500 kHz, and >1000 kHz). The shift in time of flight (TOF) and the central frequency of F(1,1) was continuously measured/monitored and their attenuation dispersion effects were correlated to the liquid level measurements at these three operating regimes. The behavior of ultrasonic guided wave mode F(1,1) when embedded with fluid at three distinct frequency ranges (i.e., >250 kHz, >500 kHz, and >1000 kHz) were studied and the use of low frequency Regime-I (250 kHz) for high range of liquid level measurements and the Regime-II (500 kHz) for low range of liquid level measurements using the F(1,1) mode with high sensitivity is reported.
Design of Deployment Strategies to Monitor the Movement of Animals with Passive Electronic Devices
Authors: Kazimierski Laila D. , Rodríguez Jorge P. , Eguíluz Víctor M.
Published: January 2021

Current animal monitoring systems have improved our knowledge of quantitative animal ecology. There are many electronic tracking technologies such as VHF/UHF telemetry, light-level geolocation, ARGOS satellite telemetry and GPS tracking. To reach the desired level of information retrieval requires the planning of adequate equipment effort and coverage, which depends on the properties of the system. We propose an equipment arrangement model consisting of a given number of receiver stations in a two-dimensional space in which the animals move according to a central place movement model. The objective is to characterize how the transmission of tracking data depends on the movement of the animals and the design of the equipment deployment: quantity and location of the receiver stations and their associated reception radius. We also implement the model using real trajectories of southern elephant seals and Australian sea lions publicly available online and tracked during the years 2010–2012. We characterize the data transmission based on different equipment configurations and we obtained analogous results to the theoretical model.
Shelf Auditing Based on Image Classification Using Semi-Supervised Deep Learning to Increase On-Shelf Availability in Grocery Stores
Authors: Yilmazer Ramiz , Birant Derya
Published: January 2021

Providing high on-shelf availability (OSA) is a key factor to increase profits in grocery stores. Recently, there has been growing interest in computer vision approaches to monitor OSA. However, the largest and well-known computer vision datasets do not provide annotation for store products, and therefore, a huge effort is needed to manually label products on images. To tackle the annotation problem, this paper proposes a new method that combines two concepts “semi-supervised learning” and “on-shelf availability” (SOSA) for the first time. Moreover, it is the first time that “You Only Look Once” (YOLOv4) deep learning architecture is used to monitor OSA. Furthermore, this paper provides the first demonstration of explainable artificial intelligence (XAI) on OSA. It presents a new software application, called SOSA XAI, with its capabilities and advantages. In the experimental studies, the effectiveness of the proposed SOSA method was verified on image datasets, with different ratios of labeled samples varying from 20% to 80%. The experimental results show that the proposed approach outperforms the existing approaches (RetinaNet and YOLOv3) in terms of accuracy.
Dryland Crop Classification Combining Multitype Features and Multitemporal Quad-Polarimetric RADARSAT-2 Imagery in Hebei Plain, China
Authors: Wang Di , Liu Chang-An , Zeng Yan , Tian Tian , Sun Zheng
Published: January 2021

The accuracy of dryland crop classification using satellite-based synthetic aperture radar (SAR) data is often unsatisfactory owing to the similar dielectric properties that exist between the crops and their surroundings. The main objective of this study was to improve the accuracy of dryland crop (maize and cotton) classification by combining multitype features and multitemporal polarimetric SAR (PolSAR) images in Hebei plain, China. Three quad-polarimetric RADARSAT-2 scenes were acquired between July and September 2018, from which 117 features were extracted using the Cloude–Pottier, Freeman–Durden, Yamaguchi, and multiple-component polarization decomposition methods, together with two polarization matrices (i.e., the coherency matrix and the covariance matrix). Random forest (RF) and support vector machine (SVM) algorithms were used for classification of dryland crops and other land-cover types in this study. The accuracy of dryland crop classification using various single features and their combinations was compared for different imagery acquisition dates, and the performance of the two algorithms was evaluated quantitatively. The importance of all investigated features was assessed using the RF algorithm to optimize the features used and the imagery acquisition date for dryland crop classification. Results showed that the accuracy of dryland crop classification increases with evolution of the phenological period. In comparison with SVM, the RF algorithm showed better performance for dryland crop classification when using full polarimetric RADARSAT-2 data. Dryland crop classification accuracy was not improved substantially when using only backscattering intensity features or polarization decomposition parameters extracted from a single-date image. Satisfactory classification accuracy was achieved using 11 optimized features (derived from the Cloude–Pottier decomposition and the coherency matrix) from 2 RADARSAT-2 images (acquisition dates corresponding to the middle and late stages of dryland crop growth). This study provides an important reference for timely and accurate classification of dryland crop in Hebei plain, China.
Improved RRT-Connect Algorithm Based on Triangular Inequality for Robot Path Planning
Authors: Kang Jin-Gu , Lim Dong-Woo , Choi Yong-Sik , Jang Woo-Jin , Jung Jin-Woo
Published: January 2021

This paper proposed a triangular inequality-based rewiring method for the rapidly exploring random tree (RRT)-Connect robot path-planning algorithm that guarantees the planning time compared to the RRT algorithm, to bring it closer to the optimum. To check the proposed algorithm’s performance, this paper compared the RRT and RRT-Connect algorithms in various environments through simulation. From these experimental results, the proposed algorithm shows both quicker planning time and shorter path length than the RRT algorithm and shorter path length than the RRT-Connect algorithm with a similar number of samples and planning time.