bluuebunny/crossref_metadata_2025 huggingface dataset:
Machine Learning Models Using Data Mining for Biomass Production from Yarrowia lipolytica Fermentation
Authors: Pensupa Nattha , Treebuppachartsakul Treesukon , Pechprasarn Suejit
Published: March 2023

In this paper, a database of biomass production from Yarrowia lipolytica fermentation is prepared and constructed using machine learning and data mining approaches. The database is curated from 15 publications and consists of 301 rows of data with 25 predictors and 1 label. The predictors include inoculum size, temperature, pH, and time, while the label is the corresponding biomass production. The database is then divided into training, validation, and test datasets and analyzed as a supervised machine learning task for regression. Twenty-six regression models are employed and compared for their performance in predicting biomass production. The best-performing model is the Matern 5/2 Gaussian process regression model, which has the lowest root-mean-squared error of 0.75 g/L, the highest R squared of 0.90, and the lowest mean absolute error of 0.52 g/L. The t-test is used to identify the most important predictors, and 14 predictors are sufficient for creating an accurate model. These 14 predictors are fermentation time, peptone, temperature, total Kjeldahl nitrogen, shaking rate, total nitrogen, inoculum size, yeast extract, crude glycerol, glucose, oil and grease, media pH, ammonium sulfate, and olive oil. This research demonstrates the application of machine learning and data mining to estimate biomass production and gives insight into which parameters are essential for Yarrowia lipolytica fermentation.
Using Process Mining to Reduce Fraud in Digital Onboarding
Authors: Silva Matheus Camilo da , Tavares Gabriel Marques , Gritti Marcos Cesar , Ceravolo Paolo , Barbon Junior Sylvio
Published: March 2023

In the context of online banking, new users have to register their information to become clients through mobile applications; this process is called digital onboarding. Fraudsters often commit identity fraud by impersonating other people to obtain access to banking services by using personal data obtained illegally and causing damage to the organisation’s reputation and resources. Detecting fraudulent users by their onboarding process is not a trivial task, as it is difficult to identify possible vulnerabilities in the process to be exploited. Furthermore, the modus operandi for differentiating the behaviour of fraudulent actors and legitimate users is unclear. In this work, we propose the usage of a process mining (PM) approach to detect identity fraud in digital onboarding using a real fintech event log. The proposed PM approach is capable of modelling the behaviour of users as they go through a digital onboarding process, while also providing insight into the process itself. The results of PM techniques and the machine learning classifiers showed a promising 80% accuracy rate in classifying users as fraudulent or legitimate. Furthermore, the application of process discovery in the event log dataset produced an insightful visual model of the onboarding process.
Crisis Management Experience from Social Media: Public Response to the Safety Crisis of Imported Aquatic Products in China during the Pandemic
Authors: Liu Ru , Liu Min , Li Yufeng , Wu Linhai
Published: March 2023

China’s outbreak related to cold-chain aquatic product quality and safety in 2020 caused public panic and further led to a crisis in China’s aquatic industry. This paper uses topic clustering and emotion analysis methods to text-mine the comments of netizens on Sina Weibo to study the main features of the public’s views on the administration’s crisis management measures and to provide experience for future imported food safety management. The findings show that for the imported food safety incident and the risk of virus infection, the public response had four types of characteristics: a higher proportion of negative emotion; a wider range of information demand; attention paid to the whole imported food industry chain; and a differentiated attitude towards control policies. Based on the online public response, countermeasures to further improve the management ability of imported food safety crises are proposed as follows: the government should pay active attention to the development trend of online public opinion; work more on exploring the content of public concern and emotion; strengthen the risk assessment of imported food and establish the classification and management measures of imported food safety events; construct the imported food safety traceability system; build a special recall mechanism for imported food safety; and improve the cooperation between government and media, enhancing the public’s trust in policies.
Who Buys Surplus Meals? An Exploratory Survey in Danish Canteens
Authors: Pandey Sujita , Budhathoki Mausam , Feng Kaixin , Thomsen Marianne , Reinbach Helene Christine
Published: March 2023

Food waste has received increasing attention over the last decade, owing to its economic, environmental, and social impacts. Much of the existing research has investigated consumers’ buying behaviour towards sub-optimal and upcycle food, but surplus meal buying behaviours are poorly understood. Thus, this study performed consumer segmentation through a modular food-related lifestyle (MFRL) instrument and determined consumers’ buying behaviour towards surplus meals in canteens employing the theory of reasoned action (TRA). A survey was conducted using a validated questionnaire from a convenient sample of 460 Danish canteen users. Four food-related lifestyle consumer segments were identified by employing k-means segmentation: Conservative (28%), Adventurous (15%), Uninvolved (12%), and Eco-moderate (45%). The Partial Least Square Structural Equation Modelling (PLS-SEM) analysis indicated that attitudes and subjective norms were significantly influencing surplus meal buying intention to further influence buying behaviour. Environmental objective knowledge was significantly influencing environmental concerns to further influence attitudes and behavioural intention. However, environmental objective knowledge had no significant influence on attitude towards surplus meals. Male consumers with higher education, those having higher food responsibility and lower food involvement, and convenience scores had higher surplus food buying behaviour. The results can be used to inform policymakers, marketers, business professionals, and practitioners to promote surplus meals in canteens or similar settings.
Super-Resolved Image of M87 Observed with East Asian VLBI Network
Authors: Tazaki Fumie , Cui Yuzhu , Hada Kazuhiro , Kino Motoki , Cho Ilje , Zhao Guang-Yao , Akiyama Kazunori , Mizuno Yosuke , Ro Hyunwook , Honma Mareki , Lu Ru-Sen , Shen Zhi-Qiang , Cui Lang , Yonekura Yoshinori
Published: March 2023

Obtaining high-resolution images at centimeter-or-longer wavelengths is vital for understanding the physics of jets. We reconstructed images from the M87 22 GHz data observed with the East Asian VLBI Network (EAVN) by using the regularized maximum likelihood (RML) method, which is different from the conventional imaging method CLEAN. Consequently, a bright core and jet extending about 30 mas to the northwest were detected with a higher resolution than in the CLEAN image. The width of the jet was 0.5 mas at 0.3 mas from the core, consistent with the width measured in the 86 GHz image in the previous study. In addition, three ridges were able to be detected at around 8 mas from the core, even though the peak-to-peak separation was only 1.0 mas. This indicates that the RML image’s spatial resolution is at least 30% higher than that of the CLEAN image. This study is an important step for future multi-frequency and high-cadence observations of the EAVN to discuss the more detailed structure of the jet and its time variability.
Investigation of Colored Film Indicators for the Assessment of the Occasional Radiation Exposure
Authors: Kudrevicius Linas , Adliene Diana , Puiso Judita , Plaga Aurimas
Published: March 2023

Occupational radiation exposure monitoring is well-established in clinical or industrial environments with various different dosimeter systems. Despite the availability of many dosimetry methods and devices, a challenge with the occasional exposure registration, which may occur due to the spilling of radioactive materials or splitting of these materials in the environment, still exists, because not every individual will have an appropriate dosimeter at the time of the irradiation event. The aim of this work was to develop radiation-sensitive films—color-changing radiation indicators, which can be attached to or integrated in the textile. Polyvinyl alcohol (PVA)-based polymer hydrogels were used as a basis for fabrication of radiation indicator films. Several organic dyes (brilliant carmosine (BC), brilliant scarlet (BS), methylene red (MR), brilliant green (BG), brilliant blue (BB), methylene blue (MB) and xylenol orange (XiO)) were used as a coloring additives. Moreover, PVA films enriched with Ag nanoparticles (PVA-Ag) were investigated. In order to assess the radiation sensitivity of the produced films, experimental samples were irradiated in a linear accelerator with 6 MeV X-ray photons and the radiation sensitivity of irradiated films was evaluated using UV–Vis spectrophotometry method. The most sensitive were PVA-BB films indicating 0.4 Gy−1 sensitivity in low-dose (0–1 or 2 Gy) range. The sensitivity at higher doses was modest. These PVA-dye films were sensitive enough to detect doses up to 10 Gy and PVA-MR film indicated stable 33.3% decolorization after irradiation at this dose. It was found that the dose sensitivity of all PVA-Ag gel films varied from 0.068 to 0.11 Gy−1 and was dependent on the Ag additives concentration. Exchange of a small amount of water with ethanol or isopropanol caused the enhancement of radiation sensitivity in the films with the lowest AgNO3 concentration. Radiation-induced color change of AgPVA films varied between 30 and 40%. Performed research demonstrated the potential of colored hydrogel films in their applications as indicators for the assessment of the occasional radiation exposure.
Electronic Modulation of the 3D Architectured Ni/Fe Oxyhydroxide Anchored N-Doped Carbon Aerogel with Much Improved OER Activity
Authors: Lu Jiaxin , Hao Wenke , Wu Xiaodong , Shen Xiaodong , Cui Sheng , Shi Wenyan
Published: March 2023

It remains a big challenge to develop non-precious metal catalysts for oxygen evolution reaction (OER) in energy storage and conversion systems. Herein, a facile and cost-effective strategy is employed to in situ prepare the Ni/Fe oxyhydroxide anchored on nitrogen-doped carbon aerogel (NiFeOx(OH)y@NCA) for OER electrocatalysis. The as-prepared electrocatalyst displays a typical aerogel porous structure composed of interconnected nanoparticles with a large BET specific surface area of 231.16 m2·g−1. In addition, the resulting NiFeOx(OH)y@NCA exhibits excellent OER performance with a low overpotential of 304 mV at 10 mA·cm−2, a small Tafel slope of 72 mV·dec−1, and excellent stability after 2000 CV cycles, which is superior to the commercial RuO2 catalyst. The much enhanced OER performance is mainly derived from the abundant active sites, the high electrical conductivity of the Ni/Fe oxyhydroxide, and the efficient electronic transfer of the NCA structure. Density functional theory (DFT) calculations reveal that the introduction of the NCA regulates the surface electronic structure of Ni/Fe oxyhydroxide and increases the binding energy of intermediates as indicated by the d-band center theory. This work provides a new method for the construction of advanced aerogel-based materials for energy conversion and storage.
Identification of a Link between Suspected Metabolic Syndrome and Cognitive Impairment within Pharmaceutical Care in Adults over 75 Years of Age
Authors: Macekova Zuzana , Fazekas Tomas , Krivosova Michaela , Dragasek Jozef , Zufkova Viera , Klimas Jan , Snopkova Miroslava
Published: March 2023

The prevalence of metabolic syndrome (MetS) and cognitive impairment (CI) is increasing with age. MetS reduces overall cognition, and CI predicts an increased risk of drug-related problems. We investigated the impact of suspected MetS (sMetS) on cognition in an aging population receiving pharmaceutical care in a different state of old age (60–74 vs. 75+ years). Presence or absence of sMetS (sMetS+ or sMetS−) was assessed according to criteria modified for the European population. The Montreal Cognitive Assessment (MoCA) score, being ≤24 points, was used to identify CI. We found a lower MoCA score (18.4 ± 6.0) and a higher rate of CI (85%) in the 75+ group when compared to younger old subjects (23.6 ± 4.3; 51%; p < 0.001). In the age group of 75+, a higher occurrence, of MoCA ≤ 24 points, was in sMetS+ (97%) as compared to sMetS− (80% p < 0.05). In the age group of 60–74 years, a MoCA score of ≤24 points was identified in 63% of sMetS+ when compared to 49% of sMetS− (NS). Conclusively, we found a higher prevalence of sMetS, the number of sMetS components and lower cognitive performance in subjects aged 75+. This age, the occurrence of sMetS and lower education can predict CI.
Effects of Exogenously Applied Copper in Tomato Plants’ Oxidative and Nitrogen Metabolisms under Organic Farming Conditions
Authors: Alves Alexandre , Ribeiro Rafael , Azenha Manuel , Cunha Mário , Teixeira Jorge
Published: March 2023

Currently, copper is approved as an active substance among plant protection products and is considered effective against more than 50 different diseases in different crops, conventional and organic. Tomato has been cultivated for centuries, but many fungal diseases still affect it, making it necessary to control them through antifungal agents, such as copper, making it the primary form of fungal control in organic farming systems (OFS). The objective of this work was to determine whether exogenous copper applications can affect AOX mechanisms and nitrogen use efficiency in tomato plant grown in OFS. For this purpose, plants were sprayed with ‘Bordeaux’ mixture (SP). In addition, two sets of plants were each treated with 8 mg/L copper in the root substrate (S). Subsequently, one of these groups was also sprayed with a solution of ‘Bordeaux’ mixture (SSP). Leaves and roots were used to determine NR, GS and GDH activities, as well as proline, H2O2 and AsA levels. The data gathered show that even small amounts of copper in the rhizosphere and copper spraying can lead to stress responses in tomato, with increases in total ascorbate of up to 70% and a decrease in GS activity down to 49%, suggesting that excess copper application could be potentially harmful in horticultural production by OFS.
Work-Related, Non-Specific Low Back Pain among Physiotherapists in France: Prevalence and Biomechanical and Psychosocial Risk Factors, as a Function of Practice Pattern
Authors: Pellissier Baptiste , Sarhan François-Régis , Telliez Frédéric
Published: March 2023

Background. Physiotherapists worldwide experience lower back pain (LBP). Up to 80% of physiotherapists report having experienced an episode of LBP at some point in their career, and LBP is the most common musculoskeletal disorder in this profession. In France, the prevalence of LBP among physiotherapists and associated work-related risk factors have not previously been studied. Objective. To determine whether the risk of work-related non-specific LBP among French physiotherapists depends on practice pattern. Method. A link to an online self-questionnaire was sent to French physiotherapists. The various practice patterns were compared with regard to the prevalence of LBP, the total number of days with LBP during the previous 12 months, and the degree of exposure to biomechanical, psychosocial and organisational risk factors. Results. Among the 604 physiotherapists included in the study, the prevalence of work-related, non-specific LBP in the previous 12 months was 40.4%. The prevalence was significantly greater among physiotherapists working in geriatrics (p = 0.033) and significantly lower in sports medicine (p = 0.010). Differences in exposure to risk factors were also found. Conclusions. The risk of non-specific LBP among French physiotherapists appears to depend on the mode of practice. All the various dimensions of risk must be taken into account. The present study could serve as a basis for more targeted research on the most exposed practices.
From Housewives to Employees, the Mental Benefits of Employment across Women with Different Gender Role Attitudes and Parenthood Status
Authors: Lu Zhuofei , Yan Shuo , Jones Jeff , He Yucheng , She Qigen
Published: March 2023

Previous studies suggest that paid employment can improve workers’ mental health status by offering a series of manifest and latent benefits (i.e., income, self-achievement and social engagement), which motivates policymakers’ ongoing promotion of labour force participation as an approach to protect women’s mental health status. This study extends the literature by investigating the mental health consequences of housewives’ transition into paid employment across different gender role attitude groups. In addition, the study also tests the potential moderating role of the presence of children in relationships. This study yields two major findings by using nationally representative data (N = 1222) from the United Kingdom Longitudinal Household Study (2010–2014) and OLS regressions. First, from the first wave to the next, housewives who transitioned into paid employment reported better mental health status than those who remained housewives. Second, the presence of children can moderate such associations, but only among housewives with more traditional gender role attitudes. Specifically, among the traditional group, the mental benefits of transition into paid employment are more pronounced among those without children. Therefore, policymakers should develop more innovative approaches to promote housewives’ mental health by considering a more gender-role-attitudes-sensitive design of future labour market policies.
Organisational Impact of a Remote Patient Monitoring System for Heart Failure Management: The Experience of 29 Cardiology Departments in France
Authors: Alami Sarah , Courouve Laurène , Lancman Guila , Gomis Pierrette , Al-Hamoud Gisele , Laurelli Corinne , Pasche Hélène , Chatellier Gilles , Mercier Grégoire , Roubille François , Delval Cécile , Durand-Zaleski Isabelle
Published: March 2023

Remote patient monitoring (RPM) for the management of patients with chronic heart failure (CHF) has been widely studied from clinical and health-economic points of view. In contrast, data on the organisational impact of this type of RPM are scarce. The objective of the present study of cardiology departments (CDs) in France was to describe the organisational impact of the Chronic Care ConnectTM (CCCTM) RPM system for CHF. An organisational impact map for health technology assessment was used to identify and define the criteria evaluated in the present survey, including the care process, equipment, infrastructure, training, skill transfers, and the stakeholders’ abilities to implement the care process. In April 2021, an online questionnaire was sent to 31 French CDs that were using CCCTM for CHF management: 29 (94%) completed the questionnaire. The survey results showed that CDs progressively modified their organisational structures upon or shortly after the implementation of the RPM device. Twenty-four departments (83%) had created a dedicated team, sixteen (55%) had provided dedicated outpatient consultations for patients with an emergency alert, and twenty-five (86%) admitted patients directly (i.e., avoiding the need to attend the emergency department). The present survey is the first to have assessed the organisational impact of the implementation of the CCCTM RPM device for CHF management. The results highlighted the variety of organisational structures, which tended to structure with the use of the device.
Getting Connected to M-Health Technologies through a Meta-Analysis
Authors: Calegari Luiz Philipi , Tortorella Guilherme Luz , Fettermann Diego Castro
Published: March 2023

The demand for mobile e-health technologies (m-health) continues with constant growth, stimulating the technological advancement of such devices. However, the customer needs to perceive the utility of these devices to incorporate them into their daily lives. Hence, this study aims to identify users’ perceptions regarding the acceptance of m-health technologies based on a synthesis of meta-analysis studies on the subject in the literature. Using the relations and constructs proposed in the UTAUT2 (Unified Theory of Acceptance and Use of Technology 2) technology acceptance model, the methodological approach utilized a meta-analysis to raise the effect of the main factors on the Behavioral Intention to Use m-health technologies. Furthermore, the model proposed also estimated the moderation effect of gender, age, and timeline variables on the UTAUT2 relations. In total, the meta-analysis utilized 84 different articles, which presented 376 estimations based on a sample of 31,609 respondents. The results indicate an overall compilation of the relations, as well as the primary factors and moderating variables that determine users’ acceptance of the studied m-health systems.
Factors Influencing the Level of Depression and Anxiety of Community-Dwelling Patients with Schizophrenia in China during the COVID-19 Pandemic
Authors: Chen Shanshan , Sun Xiaohua , Zhu Qisha , Zhao Yuan , Tang Jinsong , Song Haidong
Published: March 2023

The coronavirus disease 2019 (COVID-19) poses a huge challenge to global public health. People with schizophrenia living in communities urgently need effective interventions to help them adjust to life and work, but they have not received enough attention. This study aims to assess the prevalence of anxiety and depression symptoms in community-dwelling patients with schizophrenia in China during the epidemic and to explore the possible influencing factors. Methods: Using a cross-sectional survey, we collected 15,165 questionnaires. Assessments included demographic information, concern about COVID-19-related information, sleep status, anxiety and depressive symptoms, and accompanying illnesses. The 7-item Generalized Anxiety Disorder (GAD-7) and the 9-item Patient Health Questionnaire (PHQ-9) were used to evaluate depression and anxiety levels. Group comparison was conducted by t-test, ANOVA, or chi-square test wherever suitable, with Bonferroni pairwise correction. Multivariate logistic regression was performed to identify predictors for anxiety and depression. Results: 16.9% of patients had at least moderate anxiety, and 34.9% had at least moderate depression. T-test showed that females scored higher on GAD-7 and PHQ-9 than males, and patients without accompanying long-standing diseases, who were not concerned about COVID-19, had lower GAD-7 and PHQ-9 scores. ANOVA showed that participants aged from 30 to 39, with higher education scored higher on GAD-7, and patients with better sleep, and having less concern about COVID-19, had lower GAD-7 and PHQ-9 scores. Regression analysis indicated that participant ages of 30–39 and 40–49 positively predicted anxiety, whereas patient ages of 30–39 years positively predicted depression. Patients with poor sleep, accompanying diseases, and concern about the COVID-19 pandemic were more likely to experience anxiety and depression. Conclusion: During the pandemic, Chinese community-dwelling patients with schizophrenia had high rates of anxiety and depression. These patients deserve clinical attention and psychological intervention, especially those with risk factors.
Exploring the Effect of Emotional Labor on Turnover Intention and the Moderating Role of Perceived Organizational Support: Evidence from Korean Firefighters
Authors: Lim Jaeyoung , Moon Kuk-Kyoung
Published: March 2023

Synthesizing the conservation of resource theory, proximal withdrawal state theory, and job demands-resources theory, the present study examined the relationships between two dimensions of emotional labor (i.e., surface and deep acting) and turnover intention, as well as the moderating role of perceived organizational support in these relationships, such as the context of Korean firefighters. Using survey data drawn from fire organizations in Gyeonggi-do, the largest province of South Korea, we found that both surface and deep acting are positively related to firefighter turnover intentions. Further analysis indicates that the perceived organizational support of firefighters, vital for public health and safety, attenuates the positive relationship between surface acting and turnover intention but has no significant moderating effect on the relationship between deep acting and turnover intention. Our results suggest that perceived organizational support acts through essential psychological resources to recover the loss of emotional resources and contributes to the retention of firefighter personnel who primarily perform challenging and stressful work, including firefighting and offering emergency medical services. Thus, this study examines a crucial tool to ensure firefighters’ public mental health.
The Predictive Accuracy of the LSI-R in Female Forensic Inpatients—Assessing the Utility of Gender-Responsive Risk Factors
Authors: Wolf Viviane , Mayer Juliane , Steiner Ivonne , Franke Irina , Klein Verena , Streb Judith , Dudeck Manuela
Published: March 2023

Female reoffending has long been a neglected research interest. Accordingly, risk assessment instruments were developed based on the criminological knowledge of male recidivism. While feminist researchers have repeatedly criticized the failure to incorporate gender-responsive risk (GR) factors, opinions on the gender neutrality of existing instruments remain inconsistent. In order to substitute the existing literature, while extending the scope to mentally disordered offenders, the aim of the given study was the prediction of general recidivism in a sample of 525 female forensic inpatients who had been discharged from forensic psychiatric care in Germany between 2001 and 2018. Primarily, ROC analysis was conducted to assess the predictive accuracy of the LSI-R. Subsequently, separate binary logistic regression analyses were performed to determine the predictive utility of GR factors on recidivism. Lastly, multiple binary logistic regression was used to assess the incremental validity of the GR factors. The results showed that the GR factors (i.e., intimate relationship dysfunction, mental health issues, parental stress, adult physical abuse, and poverty) significantly contributed to the prediction of recidivism, while a mixed personality disorder, a dissocial personality, an unsupportive partner, and poverty added incremental validity to the predictive accuracy of the LSI-R. However, given that the added variables could only improve classification accuracy by 2.2%, the inclusion of gender-specific factors should be cautiously evaluated.
Effects of Online Video Sport Spectatorship on the Subjective Well-Being of College Students: The Moderating Effect of Sport Involvement
Authors: Lin Yi-Hsiu , Chen Chen-Yueh , Lin Yen-Kuang , Lee Chen-Yin , Cheng Chia-Yi
Published: March 2023

Individuals’ engagement in sports and sport-related entertainment is reported to improve their subjective well-being (SWB). We investigated whether online video sport spectatorship (OVSS) enhances the SWB of college students and whether sport involvement moderates the correlation between OVSS and SWB. For this, a pretest–posttest experimental design with a 3-week-long intervention (OVSS) was adopted. Two groups, intervention and control, were formed. The findings revealed that OVSS improved SWB (p = 0.017). Furthermore, sport involvement moderated the correlation between OVSS and SWB (p = 0.024). Specifically, participants with high levels of sport involvement in the intervention group exhibited better SWB (M = 5.51) than did the corresponding controls (M = 4.69). By contrast, among participants with low levels of sport involvement, only the intervention group showed improved SWB; however, it remained unchanged in the control group. The present study enriches the relevant literature and provides empirical evidence for the psychological benefits conferred by OVSS. Our findings may serve as a reference for designing interventions aimed at improving individuals’ overall quality of life.
Community Health Representatives as Trusted Sources for Increasing Representation of American Indian Communities in Clinical Research
Authors: Sabo Samantha , Lee Naomi , Sears Grant , Jiménez Dulce J. , Tutt Marissa , Santos Jeffersson , Gomez Omar , Teufel-Shone Nicolette , Bennet Marianne , Nashio J. T. Neva , Flores Fernando , Baldwin Julie
Published: March 2023

Indigenous and American Indian Alaskan Native (AI/AN) community members are systematically underrepresented in clinical trial research. This paper focuses on exploratory steps to partner with Native Nations of Arizona to engage Community Health Representatives (CHR) as a trusted source for building COVID-19 clinical trial research, including vaccine trials awareness. CHRs are frontline public health workers who apply a unique understanding of the experience, language, and culture of the population served. This workforce has entered the spotlight as essential to the prevention and control of COVID-19. Methods: Three Tribal CHR programs were engaged to develop and refine culturally centered educational materials and a pre-post survey using a consensus-based decision-making approach. CHRs used these materials in brief education sessions during regular client home visits and community events. Results: At 30 days post CHR intervention, participants (N = 165) demonstrated significantly increased awareness about and ability to enroll in COVID-19 treatment and vaccine trials. Participants also described a significant increase in trust in researchers, decreased perceived barriers related to cost for participation in a clinical trial, and improved belief that participation in a COVID-19 clinical trial for treatment was considered a benefit to American Indian and Alaskan Native people. Conclusion: CHRs as trusted sources of information, coupled with culturally centered education materials designed by CHRs for CHR clients, demonstrated a promising approach to improved awareness of clinical trial research generally and COVID-19 trials specifically among Indigenous and American Indian community members of Arizona.
Correlates of Social Isolation in Forensic Psychiatric Patients with Schizophrenia Spectrum Disorders: An Explorative Analysis Using Machine Learning
Authors: Machetanz Lena , Lau Steffen , Huber David , Kirchebner Johannes
Published: March 2023

The detrimental effects of social isolation on physical and mental health are well known. Social isolation is also known to be associated with criminal behavior, thus burdening not only the affected individual but society in general. Forensic psychiatric patients with schizophrenia spectrum disorders (SSD) are at a particularly high risk for lacking social integration and support due to their involvement with the criminal justice system and their severe mental illness. The present study aims to exploratively evaluate factors associated with social isolation in a unique sample of forensic psychiatric patients with SSD using supervised machine learning (ML) in a sample of 370 inpatients. Out of >500 possible predictor variables, 5 emerged as most influential in the ML model: attention disorder, alogia, crime motivated by ego disturbances, total PANSS score, and a history of negative symptoms. With a balanced accuracy of 69% and an AUC of 0.74, the model showed a substantial performance in differentiating between patients with and without social isolation. The findings show that social isolation in forensic psychiatric patients with SSD is mainly influenced by factors related to illness and psychopathology instead of factors related to the committed offences, e.g., the severity of the crime.
Placental Mesenchymal Stem Cells Alleviate Podocyte Injury in Diabetic Kidney Disease by Modulating Mitophagy via the SIRT1-PGC-1alpha-TFAM Pathway
Authors: Han Xiudan , Wang Jiao , Li Ruilin , Huang Meiling , Yue Guanru , Guan Lulu , Deng Yuanyuan , Cai Wei , Xu Jixiong
Published: March 2023

The use of mesenchymal stem cells (MSCs) has become a new strategy for treating diabetic kidney disease (DKD). However, the role of placenta derived mesenchymal stem cells (P-MSCs) in DKD remains unclear. This study aims to investigate the therapeutic application and molecular mechanism of P-MSCs on DKD from the perspective of podocyte injury and PINK1/Parkin-mediated mitophagy at the animal, cellular, and molecular levels. Western blotting, reverse transcription polymerase chain reaction, immunofluorescence, and immunohistochemistry were used to detect the expression of podocyte injury-related markers and mitophagy-related markers, SIRT1, PGC-1α, and TFAM. Knockdown, overexpression, and rescue experiments were performed to verify the underlying mechanism of P-MSCs in DKD. Mitochondrial function was detected by flow cytometry. The structure of autophagosomes and mitochondria were observed by electron microscopy. Furthermore, we constructed a streptozotocin-induced DKD rat model and injected P-MSCs into DKD rats. Results showed that as compared with the control group, exposing podocytes to high-glucose conditions aggravated podocyte injury, represented by a decreased expression of Podocin along with increased expression of Desmin, and inhibited PINK1/Parkin-mediated mitophagy, manifested as a decreased expression of Beclin1, the LC3II/LC3I ratio, Parkin, and PINK1 associated with an increased expression of P62. Importantly, these indicators were reversed by P-MSCs. In addition, P-MSCs protected the structure and function of autophagosomes and mitochondria. P-MSCs increased mitochondrial membrane potential and ATP content and decreased the accumulation of reactive oxygen species. Mechanistically, P-MSCs alleviated podocyte injury and mitophagy inhibition by enhancing the expression of the SIRT1-PGC-1α-TFAM pathway. Finally, we injected P-MSCs into streptozotocin-induced DKD rats. The results revealed that the application of P-MSCs largely reversed the markers related to podocyte injury and mitophagy and significantly increased the expression of SIRT1, PGC-1α, and TFAM compared with the DKD group. In conclusion, P-MSCs ameliorated podocyte injury and PINK1/Parkin-mediated mitophagy inhibition in DKD by activating the SIRT1-PGC-1α-TFAM pathway.
Chronic Exposure to High Fat Diet Affects the Synaptic Transmission That Regulates the Dopamine Release in the Nucleus Accumbens of Adolescent Male Rats
Authors: Plaza-Briceño Wladimir , Velásquez Victoria B. , Silva-Olivares Francisco , Ceballo Karina , Céspedes Ricardo , Jorquera Gonzalo , Cruz Gonzalo , Martínez-Pinto Jonathan , Bonansco Christian , Sotomayor-Zárate Ramón
Published: March 2023

Obesity is a pandemic caused by many factors, including a chronic excess in hypercaloric and high-palatable food intake. In addition, the global prevalence of obesity has increased in all age categories, such as children, adolescents, and adults. However, at the neurobiological level, how neural circuits regulate the hedonic consumption of food intake and how the reward circuit is modified under hypercaloric diet consumption are still being unraveled. We aimed to determine the molecular and functional changes of dopaminergic and glutamatergic modulation of nucleus accumbens (NAcc) in male rats exposed to chronic consumption of a high-fat diet (HFD). Male Sprague-Dawley rats were fed a chow diet or HFD from postnatal day (PND) 21 to 62, increasing obesity markers. In addition, in HFD rats, the frequency but not amplitude of the spontaneous excitatory postsynaptic current is increased in NAcc medium spiny neurons (MSNs). Moreover, only MSNs expressing dopamine (DA) receptor type 2 (D2) increase the amplitude and glutamate release in response to amphetamine, downregulating the indirect pathway. Furthermore, NAcc gene expression of inflammasome components is increased by chronic exposure to HFD. At the neurochemical level, DOPAC content and tonic dopamine (DA) release are reduced in NAcc, while phasic DA release is increased in HFD-fed rats. In conclusion, our model of childhood and adolescent obesity functionally affects the NAcc, a brain nucleus involved in the hedonic control of feeding, which might trigger addictive-like behaviors for obesogenic foods and, through positive feedback, maintain the obese phenotype.
Transient Receptor Potential (TRP) Channels in Pain, Neuropsychiatric Disorders, and Epilepsy
Authors: Yang Felix , Sivils Andy , Cegielski Victoria , Singh Som , Chu Xiang-Ping
Published: March 2023

Pharmacomodulation of membrane channels is an essential topic in the study of physiological conditions and disease status. Transient receptor potential (TRP) channels are one such family of nonselective cation channels that have an important influence. In mammals, TRP channels consist of seven subfamilies with a total of twenty-eight members. Evidence shows that TRP channels mediate cation transduction in neuronal signaling, but the full implication and potential therapeutic applications of this are not entirely clear. In this review, we aim to highlight several TRP channels which have been shown to mediate pain sensation, neuropsychiatric disorders, and epilepsy. Recent findings suggest that TRPM (melastatin), TRPV (vanilloid), and TRPC (canonical) are of particular relevance to these phenomena. The research reviewed in this paper validates these TRP channels as potential targets of future clinical treatment and offers patients hope for more effective care.
Depolarization and Hyperexcitability of Cortical Motor Neurons after Spinal Cord Injury Associates with Reduced HCN Channel Activity
Authors: Benedetti Bruno , Bieler Lara , Erhardt-Kreutzer Christina , Jakubecova Dominika , Benedetti Ariane , Reisinger Maximilian , Dannehl Dominik , Thome Christian , Engelhardt Maren , Couillard-Despres Sebastien
Published: March 2023

A spinal cord injury (SCI) damages the axonal projections of neurons residing in the neocortex. This axotomy changes cortical excitability and results in dysfunctional activity and output of infragranular cortical layers. Thus, addressing cortical pathophysiology after SCI will be instrumental in promoting recovery. However, the cellular and molecular mechanisms of cortical dysfunction after SCI are poorly resolved. In this study, we determined that the principal neurons of the primary motor cortex layer V (M1LV), those suffering from axotomy upon SCI, become hyperexcitable following injury. Therefore, we questioned the role of hyperpolarization cyclic nucleotide gated channels (HCN channels) in this context. Patch clamp experiments on axotomized M1LV neurons and acute pharmacological manipulation of HCN channels allowed us to resolve a dysfunctional mechanism controlling intrinsic neuronal excitability one week after SCI. Some axotomized M1LV neurons became excessively depolarized. In those cells, the HCN channels were less active and less relevant to control neuronal excitability because the membrane potential exceeded the window of HCN channel activation. Care should be taken when manipulating HCN channels pharmacologically after SCI. Even though the dysfunction of HCN channels partakes in the pathophysiology of axotomized M1LV neurons, their dysfunctional contribution varies remarkably between neurons and combines with other pathophysiological mechanisms.
Co-Immobilization of Lipases with Different Specificities for Efficient and Recyclable Biodiesel Production from Waste Oils: Optimization Using Response Surface Methodology
Authors: Wang Qian , Zhang Rongjing , Liu Maogen , Ma Lin , Zhang Weiwei
Published: March 2023

Lipase-catalyzed transesterification is a promising and sustainable approach to producing biodiesel. To achieve highly efficient conversion of heterogeneous oils, combining the specificities and advantages of different lipases is an attractive strategy. To this end, highly active Thermomyces lanuginosus lipase (1,3-specific) and stable Burkholderia cepacia lipase (non-specific) were covalently co-immobilized on 3-glycidyloxypropyltrimethoxysilane (3-GPTMS) modified Fe3O4 magnetic nanoparticles (co-BCL-TLL@Fe3O4). The co-immobilization process was optimized using response surface methodology (RSM). The obtained co-BCL-TLL@Fe3O4 exhibited a significant improvement in activity and reaction rate compared with mono and combined-use lipases, achieving 92.9% yield after 6 h under optimal conditions, while individually immobilized TLL, immobilized BCL and their combinations exhibited yields of 63.3%, 74.2% and 70.6%, respectively. Notably, co-BCL-TLL@Fe3O4 achieved 90–98% biodiesel yields after 12 h using six different feedstocks, demonstrating the perfect synergistic effect of BCL and TLL remarkably motivated in co-immobilization. Furthermore, co-BCL-TLL@Fe3O4 could maintain 77% of initial activity after nine cycles by removing methanol and glycerol from catalyst surface, accomplished by washing with t-butanol. The high catalytic efficiency, wide substrate adaptability and favorable reusability of co-BCL-TLL@Fe3O4 suggest that it will be an economical and effective biocatalyst for further applications.
Cellular 1H MR Relaxation Times in Healthy and Cancer Three-Dimensional (3D) Breast Cell Culture
Authors: Bober Zuzanna , Podgórski Rafał , Aebisher David , Cieślar Grzegorz , Kawczyk-Krupka Aleksandra , Bartusik-Aebisher Dorota
Published: March 2023

Noninvasive measurements of 1H Magnetic Resonance Imaging (MR) relaxation times in a three-dimensional (3D) cell culture construct are presented. Trastuzumab was used as a pharmacological component delivered to the cells in vitro. The purpose of this study was to evaluate the Trastuzumab delivery by relaxation times in 3D cell cultures. The bioreactor has been designed and used for 3D cell cultures. Four bioreactors were prepared, two with normal cells and two with breast cancer cells. The relaxation times of HTB-125 and CRL 2314 cell cultures were determined. An immunohistochemistry (IHC) test was performed before MRI measurements to confirm the amount of HER2 protein in the CRL-2314 cancer cells. The results showed that the relaxation time of CRL2314 cells is lower than normal HTB-125 cells in both cases, before and after treatment. An analysis of the results showed that 3D culture studies have potential in evaluating treatment efficacy using relaxation times measurements with a field of 1.5 Tesla. The use 1H MRI relaxation times allows for the visualization of cell viability in response to treatment.
Modified mRNA as a Treatment for Myocardial Infarction
Authors: Wang Yu , Wu Meiping , Guo Haidong
Published: March 2023

Myocardial infarction (MI) is a severe disease with high mortality worldwide. However, regenerative approaches remain limited and with poor efficacy. The major difficulty during MI is the substantial loss of cardiomyocytes (CMs) with limited capacity to regenerate. As a result, for decades, researchers have been engaged in developing useful therapies for myocardial regeneration. Gene therapy is an emerging approach for promoting myocardial regeneration. Modified mRNA (modRNA) is a highly potential delivery vector for gene transfer with its properties of efficiency, non-immunogenicity, transiency, and relative safety. Here, we discuss the optimization of modRNA-based therapy, including gene modification and delivery vectors of modRNA. Moreover, the effective of modRNA in animal MI treatment is also discussed. We conclude that modRNA-based therapy with appropriate therapeutical genes can potentially treat MI by directly promoting proliferation and differentiation, inhibiting apoptosis of CMs, as well as enhancing paracrine effects in terms of promoting angiogenesis and inhibiting fibrosis in heart milieu. Finally, we summarize the current challenges of modRNA-based cardiac treatment and look forward to the future direction of such treatment for MI. Further advanced clinical trials incorporating more MI patients should be conducted in order for modRNA therapy to become practical and feasible in real-world treatment.
Molecular Genetics of Abnormal Redox Homeostasis in Type 2 Diabetes Mellitus
Authors: Azarova Iuliia , Polonikov Alexey , Klyosova Elena
Published: March 2023

Numerous studies have shown that oxidative stress resulting from an imbalance between the production of free radicals and their neutralization by antioxidant enzymes is one of the major pathological disorders underlying the development and progression of type 2 diabetes (T2D). The present review summarizes the current state of the art advances in understanding the role of abnormal redox homeostasis in the molecular mechanisms of T2D and provides comprehensive information on the characteristics and biological functions of antioxidant and oxidative enzymes, as well as discusses genetic studies conducted so far in order to investigate the contribution of polymorphisms in genes encoding redox state-regulating enzymes to the disease pathogenesis.
The Pathological Activation of Microglia Is Modulated by Sexually Dimorphic Pathways
Authors: O'Connor Jennifer L. , Nissen Jillian C.
Published: March 2023

Microglia are the primary immunocompetent cells of the central nervous system (CNS). Their ability to survey, assess and respond to perturbations in their local environment is critical in their role of maintaining CNS homeostasis in health and disease. Microglia also have the capability of functioning in a heterogeneous manner depending on the nature of their local cues, as they can become activated on a spectrum from pro-inflammatory neurotoxic responses to anti-inflammatory protective responses. This review seeks to define the developmental and environmental cues that support microglial polarization towards these phenotypes, as well as discuss sexually dimorphic factors that can influence this process. Further, we describe a variety of CNS disorders including autoimmune disease, infection, and cancer that demonstrate disparities in disease severity or diagnosis rates between males and females, and posit that microglial sexual dimorphism underlies these differences. Understanding the mechanism behind differential CNS disease outcomes between men and women is crucial in the development of more effective targeted therapies.
Metal–Organic Framework-Derived Mn/Ni Dual-Metal Single-Atom Catalyst for Efficient Oxygen Reduction Reaction
Authors: Sun Zewen , Zhang Siyuan , Zheng Bo , Zhou Yue , Chen Wenshu , Liu Rui , Liu Guangxiang , Lang Leiming
Published: March 2023

Non-precious-metal-based oxygen reduction reaction (ORR) catalysts hold great prospects for rechargeable metal–air batteries and reversible electrolyzer/fuel cell systems. Among the various earth-abundant and noble-metal-free catalysts, Mn- and Ni-based single-atom catalysts (SACs) are attracting attention for ORRs. Herein, we designed a facile and efficient strategy to obtain Mn/Ni dual-metal single-atom catalysts, in which atomic Mn and Ni sites were dispersed on nitrogen-doped porous carbon. The optimized Mn/Ni catalysts showed excellent ORR electrocatalytic performance with a half-wave potential of 0.803 V, comparable to that of commercial Pt/C catalysts. Meanwhile, the electron transfer number was determined to be 3.9, indicating a good four-electron reaction process. The excellent electrocatalytic performance was attributed to the N-doped porous carbon structure with a large specific surface area, which afforded abundant active sites to anchor the single Mn and Ni atoms.