bluuebunny/crossref_metadata_2025 huggingface dataset:
Traversability analysis with vision and terrain probing for safe legged robot navigation
Authors: Haddeler Garen , Chuah Meng Yee (Michael) , You Yangwei , Chan Jianle , Adiwahono Albertus H. , Yau Wei Yun , Chew Chee-Meng
Published: August 2022

Inspired by human behavior when traveling over unknown terrain, this study proposes the use of probing strategies and integrates them into a traversability analysis framework to address safe navigation on unknown rough terrain. Our framework integrates collapsibility information into our existing traversability analysis, as vision and geometric information alone could be misled by unpredictable non-rigid terrains such as soft soil, bush area, or water puddles. With the new traversability analysis framework, our robot has a more comprehensive assessment of unpredictable terrain, which is critical for its safety in outdoor environments. The pipeline first identifies the terrain’s geometric and semantic properties using an RGB-D camera and desired probing locations on questionable terrains. These regions are probed using a force sensor to determine the risk of terrain collapsing when the robot steps over it. This risk is formulated as a collapsibility metric, which estimates an unpredictable region’s ground collapsibility. Thereafter, the collapsibility metric, together with geometric and semantic spatial data, is combined and analyzed to produce global and local traversability grid maps. These traversability grid maps tell the robot whether it is safe to step over different regions of the map. The grid maps are then utilized to generate optimal paths for the robot to safely navigate to its goal. Our approach has been successfully verified on a quadrupedal robot in both simulation and real-world experiments.
Sleep disturbance and associated factors among Nigerian adults living with HIV in the dolutegravir era
Authors: Osiyemi Adenekan O. , Owoaje Eme , Mundt Jennifer M. , Oladeji Bibilola , Awolude Oluwatosin , Ogunniyi Adesola , Okonkwo Prosper , Berzins Baiba , Taiwo Babafemi O.
Published: August 2022

Sleep disturbance is common among persons living with HIV (PLWH) causing significant health impacts. Nigeria recently switched from efavirenz to dolutegravir (DTG) for first-line antiretroviral therapy (ART). This study aimed to assess the prevalence of sleep disturbance and to determine factors associated with sleep disturbance among treatment-experienced PLWH. Using a cross-sectional study design and systematic random sampling, 300 participants were recruited from the Infectious Diseases Institute, Ibadan, Nigeria (IDI). Interviewer administered questionnaire was used to collect data regarding sociodemographic, sleep disturbance (Pittsburgh Sleep Quality Index; PSQI), depression (Patient Health Questionnaire; PHQ-9), anxiety (Generalized Anxiety Disorder-7; GAD-7), and psychoactive substance use. HIV-specific data were retrieved from IDI's medical records. The relationship between the PSQI score and the scores on the PHQ-9 and GAD-7 were explored with the Pearson correlation coefficient. Chi-square global tests of independence were used to assess factors associated with sleep disturbance and a multivariable binary logistic model was used to determine independent predictors of sleep disturbance. The mean age of the sample was 44.5 ± 11.4 (years), the average duration of HIV diagnosis was 8.13 ± 5.33 (years) and the majority were on DTG-based regimens (95%). Depression and anxiety were present in 14 and 17.3%, respectively. Prevalence of sleep disturbance (PSQI score of 6 or more) was 21.7%. Higher PSQI scores were associated with higher PHQ-9 scores (r = 0.526; p < 0.001) and higher GAD-7 scores (r = 0.529; p < 0.001). Sleep disturbance was associated with age (χ2 = 4.483, p = 0.038), marital status (χ2 = 7.187, p < 0.01), depression (χ2 = 46.589, p < 0.001), and anxiety (χ2 = 38.379, p < 0.001). There was no significant association between sleep disturbance and HIV clinical stage at diagnosis, virological suppression status, and ART regimen type (p > 0.05), whereas tea intake was associated with an absence of sleep disturbance (χ2 = 6.334, p < 0.014). Age (>45 years), depression and anxiety were associated with higher odds of sleep disturbance among PLWH. Sleep disturbance remains common among PLWH in the DTG era. Depression and anxiety are significant factors associated with sleep disturbance. Assessing these factors in future studies may improve the sleep health of PLWH.
The ParFlow Sandtank: An interactive educational tool making invisible groundwater visible
Authors: Gallagher Lisa K. , Farley Abram J. , Chennault Calla , Cerasoli Sara , Jourdain Sébastien , O'Leary Patrick , Condon Laura E. , Maxwell Reed M.
Published: August 2022

Physical aquifer models are a highly effective teaching tool for hydrology education, however they come with inherent limitations that include the high cost to purchase, the static configuration of the model materials, the time required to visualize hydrogeological phenomena, and the effort to reset and clean them over time. To address these and other limitations, we have developed an interactive computer simulation of a physical aquifer model called the ParFlow Sandtank. In this gamified interface, users run the simulation using a familiar web-app like interface with sliders and buttons while learning real hydrologic concepts. Our user interface allows participants to dive into the world of hydrology, understanding assumptions about model parameters such as hydraulic conductivity, making decisions about inputs to groundwater aquifer systems such as pumping rates, visualizing outputs such as stream flow, transport, and saturation, and exploring various factors that impact real environmental systems such as climate change. The ParFlow Sandtank has already been used in a variety of educational settings with more than 9,000 users per year, and we feel this emerging educational tool can be used broadly in educational environments and can be scaled-up to provide greater accessibility for students and educators. Here we present the capabilities and workflow of the ParFlow Sandtank, two use cases, and additional tools and custom templates that have been developed to support and enhance the reach of the ParFlow Sandtank.
Safety of exercise for adults with thoracic aortic aneurysms and dissections
Authors: Li Jesse , Boyd Alexandra , Huang Michael , Berookhim Joshua , Prakash Siddharth K.
Published: August 2022

Background

Current guidelines for patients with thoracic aortic aneurysms or dissections (TAD) restrict vigorous exertion with the intention to prevent acute aortic dissections. However, a safe threshold for exercise intensity has not been established for TAD patients. In this study, we measured exertional changes in systolic and diastolic blood pressure during isometric and dynamic exercises in a cohort of TAD patients to determine safety of moderate intensity exercise.

Methods

Thirty-one adults with TAD and 14 controls were recruited from UTHealth outpatient clinics. All participants completed an exercise protocol consisting of two circuits of five moderate intensity exercises: hand grips, leg raises, bicep curls, stationary cycling, and wall sits. Blood pressure values were recorded during exercise using Spacelabs OnTrak Ambulatory Blood Pressure monitors. Perceived exertion during each exercise was measured using the Borg CR-10 scale.

Results

No significant differences in the maximum exertional systolic pressure, diastolic pressure, or change from baseline was found between the TAD and control groups. Higher amounts of self-reported weekly moderate activity level (MAL) in TAD correlated with lower exertional SBP during exercise. Higher Borg scores were associated with a greater change in systolic pressure.

Conclusion

Moderate intensity exercise is safe and feasible for many TAD patients. Our data confirms that the Borg score may be a useful proxy for exercise intensity. In this study, we establish a reproducible exercise protocol that can be adapted to create individualized exercise regimens for TAD patients as part of a care plan to improve long-term cardiovascular health.
Local diversification enhances pollinator visitation to strawberry and may improve pollination and marketability
Authors: Sciligo Amber R. , M'Gonigle Leithen K. , Kremen Claire
Published: August 2022

Numerous studies show that semi-natural habitats within agricultural landscapes benefit native pollinating insects and increase resultant crop pollination services. More recently, evidence is emerging that agricultural diversification techniques on farms, as well as increased compositional and configurational heterogeneity within the cropped portion of landscapes, enhance pollinator communities. However, to date, only a few studies have investigated how diversifying the crops within the farm field itself (i.e., polyculture) influences wild pollinator communities and crop pollination services. In the Central Coast of California, we investigate how local crop diversification within fields, crossed with the proportion of natural habitat in the surrounding landscape, jointly affect pollinator communities and services to strawberry. On 16 organic farms varying in farm type (monoculture vs. polyculture) and proportion of natural land cover, we find that both factors enhance pollinator abundance and richness, although neither affect honey bee abundance. Further, natural cover has a stronger effect on pollinator richness on monoculture (vs. polyculture) farms. Although strawberry can self-pollinate, we document experimentally that pollinator exclusion doubles the probability of berry malformation, while excluding both pollinators and wind triples malformation, with corresponding effects on berry marketability. Finally, in post-hoc tests, we find that berry malformation is significantly higher with greater visitation by honey bees, and observed a trend that this reduction was mitigated by increased native bee richness. These results suggest that both polyculture and semi-natural habitat cover support more abundant and diverse pollinator communities, and that ambient levels of pollinator visitation to strawberry provide an important crop pollination service by improving berry marketability (i.e., by reducing berry malformation). Although further confirmation would be needed, our work suggests that honey bees alone do not provide sufficient pollination services. Prior work has shown that honey bees tend to visit only the top of the strawberry flower receptacle, while other native bees often crawl around the flower base, leading to more complete pollination of the achenes and, consequently, better formed berries. If honey bee visits reduced native bee visitation in our system, this could explain the unexpected correlation between increased honey bee visits and malformation.
Case report: The first description of a Dieulafoy's lesion in the gastric mucosa of a dog
Authors: Barrantes Murillo Daniel Felipe , Tillson Michael , Koehler Jennifer W. , Sandey Maninder
Published: August 2022

An approximately 12-year-old, 31 kg, male neutered Labrador Retriever was presented to the referring hospital with an acute onset (less than 1 day) of hematemesis and melena. The dog was treated supportively for a presumptive gastric ulcer for 4 days with intravenous fluids, gastro protectants, such as pantoprazole, misoprostol, sucralfate, and barium, as well as an anti-emetic (maropitant) and analgesics (fentanyl, gabapentin, and tramadol). Throughout medical management, the dog continued to require blood transfusions approximately every 24 h. Given the poor medical response, the patient was subjected to an exploratory laparotomy. During surgery, a grossly raised, blister-like lesion on the mucosal surface of the stomach was appreciated on the lesser curvature of the stomach. A partial gastrectomy was performed, and the segment was submitted for histological evaluation. Histologically, there were multiple, tortuous, medium-caliber muscular arteries (>1.0 mm in diameter) in the submucosa. A single large-caliber artery (>0.75 mm in diameter) containing a partially occlusive thrombus extruded through the mucosa and projected on the ulcerated surface. The patient's signs were similar clinically and histopathologically to Dieulafoy's lesion in people. A Dieulafoy's lesion is a potentially life-threatening disorder that causes gastrointestinal (GI) hemorrhage. This lesion is characterized by a dilated, large-caliber, aberrant submucosal artery that erodes through the epithelium and ruptures, resulting in massive and potentially fatal hemorrhage. This lesion has never been documented previously in a dog.
The miRNome of canine invasive urothelial carcinoma
Authors: Varvil Mara S. , Bailey Taylor , Dhawan Deepika , Knapp Deborah W. , Ramos-Vara José A. , Santos Andrea P. dos
Published: August 2022

Urothelial carcinoma (UC) comprises up to 2% of all naturally occurring neoplasia in dogs and can be challenging to diagnose. MicroRNAs (miRNAs) have been reported to be dysregulated in numerous diseases, including neoplasia. MiRNA expression has been evaluated in human UC, but there is limited information regarding the miRNA transcriptome of UC in dogs. Our study aimed to evaluate differential miRNA expression in bladder tissue collected from normal canine urothelium and canine invasive UC (iUC) to elucidate the dysregulated pathways in canine UC. Next-Generation RNA sequencing (RNA-Seq) was performed for dogs with UC (n = 29) and normal canine urothelium (n = 4). Raw RNA data were subjected to normalization, and pairwise comparison was performed using EdgeR with Benjamini-Hochberg FDR multiple testing correction (p < 0.05; >2-fold change) comparing tissue samples of normal urothelium to canine iUC samples. Principal component analysis and hierarchical cluster analysis were performed. MiRNA of FFPE tissue samples of separate iUC (n = 5) and normal urothelium (n = 5) were used to evaluate five miRNAs using RT-qPCR. Pathway analysis was performed utilizing miRWalk, STRING database, and Metascape utilizing KEGG pathways and GO terms databases. Twenty-eight miRNAs were differentially expressed (DE) by RNA-Seq. RT-qPCR confirmed that four miRNAs are significantly downregulated in UC compared to healthy urothelial samples (miR-105a, miR-143, miR-181a, and miR-214). Principal component analysis and hierarchical cluster analysis showed separation between miRNAs in iUC and the control group. The DE miRNAs are most often associated with gene silencing by miRNA, miRNAs in cancer, and miRNAs involved in DNA damage responses. Proteins involved include HRAS, KRAS, ARAF, RAF1, MAPK1, MAP2K1, MAPK3, FGFR3, EGFR, HBEGF, RASSF1, E2F2, E2F3, ERBB2, SRC, MMP1, and UP3KA. The differential expression of miRNAs in canine iUC compared to normal canine urothelial tissue indicates that these markers should be further evaluated for their potential role as diagnostic and therapeutic targets.
Neural Network Based Model Predictive Control for a Quadrotor UAV
Authors: Jiang Bailun , Li Boyang , Zhou Weifeng , Lo Li-Yu , Chen Chih-Keng , Wen Chih-Yung
Published: August 2022

A dynamic model that considers both linear and complex nonlinear effects extensively benefits the model-based controller development. However, predicting a detailed aerodynamic model with good accuracy for unmanned aerial vehicles (UAVs) is challenging due to their irregular shape and low Reynolds number behavior. This work proposes an approach to model the full translational dynamics of a quadrotor UAV by a feedforward neural network, which is adopted as the prediction model in a model predictive controller (MPC) for precise position control. The raw flight data are collected by tracking various pre-designed trajectories with PX4 autopilot. The neural network model is trained to predict the linear accelerations from the flight log. The neural network-based model predictive controller is then implemented with the automatic control and dynamic optimization toolkit (ACADO) to achieve real-time online optimization. Software in the loop (SITL) simulation and indoor flight experiments are conducted to verify the controller performance. The results indicate that the proposed controller leads to a 40% reduction in the average trajectory tracking error compared to the traditional PID controller.
Short-Term Trajectory Prediction Based on Hyperparametric Optimisation and a Dual Attention Mechanism
Authors: Ding Weijie , Huang Jin , Shang Guanyu , Wang Xuexuan , Li Baoqiang , Li Yunfei , Liu Hourong
Published: August 2022

Highly accurate trajectory prediction models can achieve route optimisation and save airspace resources, which is a crucial technology and research focus for the new generation of intelligent air traffic control. Aiming at the problems of inadequate extraction of trajectory features and difficulty in overcoming the short-term memory of time series in existing trajectory prediction, a trajectory prediction model based on a convolutional neural network-bidirectional long short-term memory (CNN-BiLSTM) network combined with dual attention and genetic algorithm (GA) optimisation is proposed. First, to autonomously mine the data association between input features and trajectory features as well as highlight the influence of important features, an attention mechanism was added to a conventional CNN architecture to develop a feature attention module. An attention mechanism was introduced at the output of the BiLSTM network to form a temporal attention module to enhance the influence of important historical information, and GA was used to optimise the hyperparameters of the model to achieve the best performance. Finally, a multifaceted comparison with other typical time-series prediction models based on real flight data verifies that the prediction model based on hyperparameter optimisation and a dual attention mechanism has significant advantages in terms of prediction accuracy and applicability.
Regional Differences of Farmers’ Willingness to Grow Grain and Its Influencing Factors in Shandong Province under the Background of New-Type Urbanization
Authors: Zhang Xufang , Zhao Minghua , Wang Xiaojie , Han Rongqing
Published: August 2022

Taking Shandong Province as the research area, we explored the willingness of farmers to grow grain and the influencing factors. By constructing the evaluation system of their willingness with 6 levels and 15 indicators based on field investigation, and quantitatively analyzing the influence degree and impact assessment of factors through a logistic regression model, the regional differences in farmers’ willingness were summarized, and the influencing factors were recognized. This study indicates that there were obvious regional differences in farmers’ willingness, which were the highest in the western region, the second in the eastern region, and the lowest in the central region. Specifically, the willingness varies significantly among cities, among which Laiwu has the highest willingness (0.76), while Tai’an has the lowest (0.41). The level of urbanization in different regions and the main influencing factors are different, and the same factor has different degrees of influence on cities, leading to regional differences. In terms of urbanization level, the main influencing factors in areas with high urbanization levels are the proportion of grain income and grain expenditure. However, in areas with a low urbanization level, it is the farmers’ planting attitude. From the perspective of influence mode, different factors have positive and negative differences in the willingness. Additionally, farmers’ willingness is becoming more and more rational, and more consideration is given to economic benefits. Among the influencing factors, the land planting mode, the proportion of grain income, and the proportion of grain expenditure are the most important factors, and 82% (11) of the cities are affected by the above three factors. Finally, the corresponding incentive measures are proposed by the regional differences in the influencing factors in various cities.
Optimizing Tillage and Fertilization Patterns to Improve Soil Physical Properties, NUE and Economic Benefits of Wheat-Maize Crop Rotation Systems
Authors: Zhang Daijing , Hao Xinru , Fan Zhiyao , Hu Xiao , Ma Jianhui , Guo Yuxin , Wu Lin
Published: August 2022

Winter wheat and summer maize rotation is the main cropping pattern in the North China Plain (NCP). There are still problems with farmers’ production modes, including shallow tillage layers, single application of chemical fertilizer causing plow bottom layer thickening and soil pH decrease. A two-factor location experiment was conducted to investigate the effects of different tillage and fertilization patterns on the soil physical properties, soil organic carbon (SOC), nitrogen-use efficiency, and crop yield of wheat–maize rotation systems during the years 2018–2020. The different treatments were deep tillage + organic fertilizer (DTF), shallow tillage + organic fertilizer (STF), no tillage + organic fertilizer (NTF), deep tillage + nitrogen fertilizer (DT), shallow tillage + nitrogen fertilizer (ST), and no tillage + nitrogen fertilizer (NT). The results showed that STF treatment could effectively improve the physical properties of soil and, SOC content, and increase both the crop yield and revenue of farmers. In the STF treatment, soil water content was highest in the 0–20 cm layer (2018), which was 4.89–11.31% higher than that of the other treatments; additional organic fertilizer application reduced soil bulk and increased the proportion of <0.25 mm aggregates; SOC and soil total nitrogen (TN) content were highest in the 20–40 cm layer, (15.82–32.63% and 28.57–42.86%, respectively). The total yield of wheat–maize rotation for both years was the highest under STF treatment. The annual economic benefits under this treatment were 42,182.26 and 42,254.54 CNY ha−1, which were 1.02–12.94% and 2.29–9.87% higher than those of the other treatments. Therefore, the suggested planting method in the NCP is tillage of over 20 cm and additional organic fertilizer.
Advances in Applications of Cereal Crop Residues in Green Concrete Technology for Environmental Sustainability: A Review
Authors: Suhail Md Amir , Shrivastava Sandeep , Paritosh Kunwar , Pareek Nidhi , Kovalev Andrey A. , Kovalev Dmitriy A. , Litti Yuri V. , Panchenko Vladimir , Bolshev Vadim , Vivekanand Vivekanand
Published: August 2022

Concrete is mainly employed as a construction material. Due to the manufacturing of cement and the extent of concrete usage, numerous environmental issues and water suction have presented challenges. There is an immediate need to overcome these problematic issues by substituting natural resources with wastes and by-products of different biological processes in the production of concrete in order to make green concrete. Green concrete provides a relatively low-impact material to satisfy potential concrete demand and offers a cheaper, robust and highly reliable alternative that could fulfil future construction requirements in an environmentally safer way. The present review highlights the possible use of waste residues of agricultural origin from cereal farming in concrete as alternative materials to cement, fine aggregate and fiber reinforcement. The review also considers appropriate methods of treatment, the selection of residual resources and the blending ratios that may allow the development of next-generation green concrete with better physicochemical and mechanical properties. It also explores in-depth studies and the wider range of innovations in cereal farming residues for appropriate use in green construction for environmental sustainability. Green concrete could be an alternative material that could replace those used in conventional methods of construction and help make a further step towards environmental sustainability and a circular bioeconomy.
Osmopriming Combined with Boron-Tolerant Bacteria (Bacillus sp. MN54) Improved the Productivity of Desi Chickpea under Rainfed and Irrigated Conditions
Authors: Mehboob Noman , Yasir Tauqeer Ahmad , Hussain Shahid , Farooq Shahid , Naveed Muhammad , Hussain Mubshar
Published: August 2022

Chickpeas are rich source of protein and predominantly grown in boron (B)-deficient sandy-loam soils in Pakistan. Boron-tolerant bacteria (BTB) could tolerate higher B levels in soil and increase B availability to the plants. Field trials were conducted under irrigated (district Layyah) and rainfed (district Chakwal) conditions to evaluate the interactive effects of pre-optimized B application methods and BTB (Bacillus sp. MN54) on the nodule’s population, grain quality, productivity, and grain-B concentration in desi chickpea during 2019–2020 and 2020–2021. Boron was applied as soil application (1 kg B ha−1), foliar application (0.025% B), osmopriming (0.001% B), and seed coating (1.5 g B kg−1 seed) with or without BTB inoculation. Untreated seeds receiving no B through any of the methods were regarded as control. The individual and interactive effects (up to three-way interaction of location × BTB inoculation × B application methods) of year, location, B application methods and BTB inoculation significantly altered the growth and yield-related traits of desi chickpea. The four-way interaction of year × location × BTB inoculation × B application methods was non-significant for all recorded growth and yield-related traits. Regarding individual effects, the higher values of growth and yield-related traits were noted for 2020–2021, rainfed location, BTB inoculation and B application through seed priming. Similarly, in two-way interactions 2020–2021 with rainfed location and BTB inoculation, rainfed location with BTB inoculation and osmopriming and osmopriming with BTB inoculation recorded higher values of the growth and yield-related traits. Osmopriming combined with BTB inoculation significantly improved dry matter accumulation and leaf area index in both locations. Boron application through all the methods significantly improved grain quality, yield grain B concentration. The highest grain and biological yields, and nodules’ population were recorded with osmopriming followed by soil application of B combined with BTB inoculation. The highest plant B concentration (75.05%) was recorded with foliar application of B followed by osmopriming (68.73%) combined with BTB inoculation. Moreover, the highest economic returns (USD 2068.5 ha−1) and benefit–cost ratio (3.7%) were recorded with osmopriming + BTB inoculation in 2020–2021 under rainfed conditions. Overall, B application through osmopriming and soil application combined with BTB inoculation could be used to increase productivity and profitability of desi chickpea, whereas foliar application is a better method to enhance grain and plant B concentration.
Silicon and Nitric Oxide Applications Allow Mitigation of Water Stress in Myrobalan 29C Rootstocks (Prunus cerasifera Ehrh.)
Authors: Bolat Ibrahim , Bakır Asuman Gundogdu , Korkmaz Kubra , Gutiérrez-Gamboa Gastón , Kaya Ozkan
Published: August 2022

(1) Background: Silicon (Si) and nitric oxide (NO) have been proven to protect against cellular injury caused by stress conditions, mostly by salinity and water stress in agriculture. (2) Methods: The goal was to study the effect of soil applications of NO, Si, and their combination (Si+NO) on the response of Myrobalan 29C rootstocks (Prunus cerasifera Ehrh.) subjected to water stress and well-watered conditions. (3) Results: The results showed that water stress decreased growth parameters (i.e., leaf area, shoot dry weight, root fresh weight, shoot fresh weight, root dry weight, and relative shoot diameter), physio-biochemical parameters (i.e., chlorophyll and relative water content in leaves), and leaf and root minerals (i.e., P, Ca, Fe, and Zn in leaves and N, K, Ca, and Zn in roots), compared to well-watered plants. Under these conditions, all treatments mitigated the detrimental effects of water stress on Myrobalan 29C rootstocks, being the most effective the Si+NO treatment. (4) Conclusions: These findings briefly highlight that the combination of silicon and nitric oxide may provide greater tolerance to water stress in Myrobalan 29C rootstocks.
Optimization of Dairy Cattle Breeding Programs with Genotype by Environment Interaction in Kenya
Authors: Wahinya Peter K. , Jeyaruban Gilbert M. , Swan Andrew A. , van der Werf Julius H. J.
Published: August 2022

Genotype by environment interaction influences the effectiveness of dairy cattle breeding programs in developing countries. This study aimed to investigate the optimization of dairy cattle breeding programs for three different environments within Kenya. Multi-trait selection index theory was applied using deterministic simulation in SelAction software to determine the optimum strategy that would maximize genetic response for dairy cattle under low, medium, and high production systems. Four different breeding strategies were simulated: a single production system breeding program with progeny testing bulls in the high production system environment (HIGH); one joint breeding program with progeny testing bulls in three environments (JOINT); three environment-specific breeding programs each with testing of bulls within each environment (IND); and three environment-specific breeding programs each with testing of bulls within each environment using both phenotypic and genomic information (IND-GS). Breeding strategies were evaluated for the whole industry based on the predicted genetic response weighted by the relative size of each environment. The effect of increasing the size of the nucleus was also evaluated for all four strategies using 500, 1500, 2500, and 3000 cows in the nucleus. Correlated responses in the low and medium production systems when using a HIGH strategy were 18% and 3% lower, respectively, compared to direct responses achieved by progeny testing within each production system. The JOINT strategy with one joint breeding program with bull testing within the three production systems produced the highest response among the strategies using phenotypes only. The IND-GS strategy using phenotypic and genomic information produced extra responses compared to a similar strategy (IND) using phenotypes only, mainly due to a lower generation interval. Going forward, the dairy industry in Kenya would benefit from a breeding strategy involving progeny testing bulls within each production system.
An Integration of Transcriptomic Data and Modular Gene Co-Expression Network Analysis Uncovers Drought Stress-Related Hub Genes in Transgenic Rice Overexpressing OsAbp57
Authors: Abdullah-Zawawi Muhammad-Redha , Tan Lay-Wen , Ab Rahman Zuraida , Ismail Ismanizan , Zainal Zamri
Published: August 2022

Auxin receptor plays a significant role in the plant auxin signalling pathway in response to abiotic stress. Recently, we found that transgenic rice overexpressing ABP57 had higher drought tolerance than the wild-type cultivar, MR219, due to the fact of its enhanced leaf photosynthetic rate and yields under drought stress. We performed a microarray study on this line to investigate the underlying mechanisms contributing to the observed phenotype. After microarray data filtering, 3596 genes were subjected to modular gene co-expression network (mGCN) development using CEMiTool, an R package. We identified highly related genes in 12 modules that could act to specific responses towards drought or any of the abiotic stress types. Gene set enrichment and overrepresentation analyses for modules extracted two highly upregulated modules that are involved in drought-related biological processes such as transmembrane transport of metal ions and response to oxidative stress. Finally, 123 hub genes were identified in all modules after integrating co-expression information with physical interaction data. In addition, the interplay of significant pathways between the metabolism of chlorophyll and flavonoid and the signalling pathways of MAPK, IAA, and SA inferred the concurrent involvement of stress tolerance response. Collectively, our findings seek new future directions for breeding strategies in rice tolerant improvements.
Comparing Selection Criteria to Select Grapevine Clones by Water Use Efficiency
Authors: Mairata Andreu , Tortosa Ignacio , Douthe Cyril , Escalona José Mariano , Pou Alicia , Medrano Hipólito
Published: August 2022

The current climate change is forcing growth-adapted genotypes with a higher water use efficiency (WUE). However, the evaluation of WUE is being made by different direct and indirect parameters such as the instantaneous leaf WUE (WUEi) and isotopic discrimination of carbon (δ13C) content of fruits. In the present work, WUE has been evaluated in these two ways in a wide collection of grapevine genotypes, including Tempranillo and Garnacha clones, and Tempranillo on different rootstocks (T-rootstocks). A total of 70 genotypes have been analysed in four experimental fields over two years. The parameters used to measure WUE were the bunch biomass isotopic discrimination (δ13C) and the intrinsic WUE (WUEi), defined as the ratio between net CO2 assimilation and stomatal conductance. The genotypes with the highest and lowest WUE were identified, differences between them being found to be of more than 10%. Generally, the two parameters showed coincidences in the clones with the highest and lowest WUE, suggesting that both are valuable tools to classify genotypes by their WUE in grapevine breeding programs. However, δ13C seemed to be a better indicator for determining WUE because it represents the integration over the synthesis time of the sample analysed (mainly sugars from ripening grapes), which coincides with the driest period for the crop. Moreover, the WUEi is a variable parameter in the plant and it is more dependent on the environmental conditions. The present work suggests that carbon isotopic discrimination could be an interesting parameter for the clonal selection criteria in grapevines by WUE. The main reasons were its better discrimination between clones, the fact that sampling is less time-consuming and easier to do than WUEi, and that the samples can be stored for late determinations, increasing the number of samples that can be analysed.
Diversity in Acidity between Core and Pulp of Asian Pear Fruit Is Mainly Regulated by the Collaborative Activity of PH8.1 and DIC2 Genes during Fruit Development
Authors: Alam Shariq Mahmood , Liu Dong-Hai , Ateeq Muhammad , Han Han , Chen Huan , Khan Muhammad Abbas , Luo Yin , Chen Xue-Ling , Liu Yong-Zhong
Published: August 2022

The pear (Pyrus pyrifolia) is an important accessory fruit in which the pear core is tarter than the pear pulp. However, the reason for the acidic core and diversity in the taste of the same fruit is not clear. In this study, we observed that the citrate contents were three times higher in the core than in the pulp, while the malate content decreased along with fruit development and was significantly lower in the core than in the pulp at 110 days after flowering. Overall transcript levels for citrate-malate synthesis-related genes increased more in the pear core than the pulp at early fruit development, while degradation-related genes activity was nearly similar or non-significant between the core and pulp during fruit development. The lesser malate accumulation in the pear core compared to the pulp at 110 DAF was possibly due to the reduced activity of tDT2 gene. Regarding citrate accumulation, we identified five important p-type H+-ATPase genes in pear and found that the relative expression level of the PH8.1 gene was four-fold higher in the core than in the pulp during fruit development. Moreover, the expression level of di-carboxylate carrier gene 2 (DIC2) was constantly and significantly higher in the core than in the pulp. In addition, correlation analysis signified that the transcript levels of the two genes PH8.1 and DIC2 positively and significantly correlated with the citrate contents. These results suggested that the increased and collaborative activity of PH8.1 and DIC2 played a key role in the higher citrate accumulation in the core than the pulp, thus, with the help of molecular breeding tools, the citrate contents can be optimized in pear fruit for divers and improved fruit flavoring.
Vine Physiology, Yield Parameters and Berry Composition of Sangiovese Grape under Two Different Canopy Shapes and Irrigation Regimes
Authors: Valentini Gabriele , Pastore Chiara , Allegro Gianluca , Mazzoleni Riccardo , Chinnici Fabio , Filippetti Ilaria
Published: August 2022

Vitis vinifera L. adapts well to a scarce availability of water in the soil. However, in recent decades, the combination of thermal stress with prolonged water scarcity could have dramatic consequences on the vine’s physiological status. In this paper, we evaluated the effects of two canopy shapes and two irrigation regimes at veraison on vine physiology, yield parameters and grape composition through biochemical and molecular approaches. The water shortage strongly influenced the physiology of Sangiovese only when the stress was moderate to severe. Neither the water stress limited to veraison nor the canopy shape were able to influence the yield parameters and sugar content, and a strong induction of the expression of the genes involved in the biosynthesis of anthocyanins was recorded only in conditions of moderate-to-severe stress. This phenomenon led to an increase in the anthocyanin content in berry skins until the end of veraison. Conversely, no significant effects occurred in terms of biochemical and molecular performance after re-watering and at harvest. Though the shape of the canopy could play a role only under elevated temperature and prolonged drought, severe water stress can affect the vine physiology and berry ripening during the veraison stage.
Seed Characteristics Affect Low-Temperature Stress Tolerance Performance of Rapeseed (Brassica napus L.) during Seed Germination and Seedling Emergence Stages
Authors: Luo Tao , Sheng Ziwei , Zhang Chunni , Li Qin , Liu Xiaoyan , Qu Zhaojie , Xu Zhenghua
Published: August 2022

Screening and breeding elite varieties with rapid germination and uniform seedling emergence under low temperature is an effective strategy to deal with the cold climate occurring under late sowing conditions in the Yangtze River basin. This study focused on the performance of seven functional traits, including percentage of germination, percentage of emergence, mean germination time, mean emergence time, total seedling length, total dry weight, and seedling vigor index of 436 natural rapeseed populations under normal-temperature (25/20 °C) and low-temperature (15/10 °C) conditions. Furthermore, ten genotypes were screened to verify their low-temperature tolerance based on cultivar traits in a pot experiment. The results show that the germination- and emergence-related functional traits of rapeseed genotypes exhibit rich genotypic diversity in response to low-temperature stress; the variation among these traits ranged from 1–25% under normal-temperature and 10–49% under low-temperature conditions. Variation in seed characteristics also affected the capacity for low-temperature tolerance in the process of seed germination and seedling emergence, and could explain 22% of the total variance for low-temperature stress tolerance indices. There existed high correlations between the stress tolerance index of total dry weight (STI_TDW) and thousand-seed weight, and between the stress tolerance index of emergence percentage (STI_PE) and oil content. The contents of erucic acid, glucosinolate, and eicosenoic acid were positively correlated with the stress tolerance index of mean germination time (STI_MGT) and mean emergence time (STI_MET). The D-CRITIC (distance-based intercriteria correlation) weight method was selected in this experiment to calculate each variety’s comprehensive low-temperature stress tolerance index by integrating the standard deviation and distance correlation coefficient of each index. The genotypes with large comprehensive low-temperature stress tolerance index also had higher low-temperature stress tolerance index of biomass and yield in the pot experiment, indicating that the comprehensive low-temperature stress tolerance index has high reliability and applicability. This study could provide a theoretical basis for the utilization of low-temperature-tolerant germplasm resources, as well as a reference for the cold resistance and yield stability under late- and direct-sowing conditions of rapeseed in the Yangtze River basin and other similar environments around the world.
Remote Sensing-Based Evaluation of Heat Stress Damage on Paddy Rice Using NDVI and PRI Measured at Leaf and Canopy Scales
Authors: Ryu Jae-Hyun , Oh Dohyeok , Ko Jonghan , Kim Han-Yong , Yeom Jong-Min , Cho Jaeil
Published: August 2022

Extremely high air temperature at the heading stage of paddy rice causes a yield reduction due to the increasing spikelet sterility. Quantifying the damage to crops caused by high temperatures can lead to more accurate estimates of crop yields. The remote sensing technique evaluates crop conditions indirectly but provides information related to crop physiology, growth, and yield. In this study, we aim to assess the crop damage caused by heat stress in paddy rice examined under elevated air temperatures in a temperature gradient field chamber from 2016 to 2019, using remote-sensed vegetation indices. A leaf-spectrometer, field-spectrometers, and a multi-spectral camera were used to monitor the conditions of paddy rice. Although, in the leaf- and canopy-scales, the values of normalized difference vegetation index (NDVI) and photochemical reflectance index (PRI) decreased after the heading of rice under normal conditions, the decreasing sensitivity of NDVI and PRI was different depending on the degree of physiological heat stress by high temperature conditions. The NDVI after the heading under extremely high air temperature was not dropped and remained the value before heading. The PRI decreased at all air temperature conditions after the heading; the PRI of the plot exposed to the elevated air temperature was higher than that under ambient air temperature. Further, the relative change in NDVI and PRI after the heading exhibited a strong relationship with the ripening ratio of paddy rice, which is the variable related to crop yield. These remote-sensing results aid in evaluating the crop damage caused by heat stress using vegetation indices.
Seed Soaking with Sodium Selenate as a Biofortification Approach in Bread Wheat: Effects on Germination, Seedling Emergence, Biomass and Responses to Water Deficit
Authors: Rocha Luís , Silva Ermelinda , Pavia Ivo , Ferreira Helena , Matos Carlos , Osca José M. , Moutinho-Pereira José , Lima-Brito José
Published: August 2022

Selenium (Se) biofortification by seed treatments has been poorly explored in wheat due to the difficulties in establishing seed treatments without compromising plant productive traits. We investigated the effects of Se seed soaking as a pre-sowing treatment in bread wheat. Five soaking periods and six Se concentrations were assessed on germination and seedling traits and compared to unsoaked seeds. Twelve hours of soaking was found beneficial for most tested Se concentrations. Then, we evaluated the effects of untreated, 0, 2.5 and 25 mM Se in 12 h seed soaking treatments along the wheat crop cycle under water-deficit (WD) and well-watered (WW) conditions in a pot experiment. Our results evidenced that 12 h of 2.5 mM Se soaking did not affect the germination percentage, and speed-up seedling emergence resulted in a considerable Se seed uptake. These plants also displayed enhanced antioxidant capacity and vegetative biomass accumulation, especially under WD. The treatment with 25 mM of Se negatively affected aerial biomass, suggesting potential toxicity. Physiological responses of Se-treated plants remained unchanged, as well as grain traits. Altogether, we propose that 12 h soaking with 2.5 mM Se is a promissory pre-sowing approach to enrich bread wheat grain and straw, particularly under water-limited environments.
Assessment of the Impact on 20 Pelagic Fish Species by the Taiwanese Small-Scale Longline Fishery in the Western North Pacific Using Ecological Risk Assessment
Authors: Liu Kwang-Ming , Huang Lung-Hsin , Su Kuan-Yu
Published: August 2022

Ecological risk assessment (ERA) has been applied on assessing the relative risk of bycatch species in recent years. ERA index is calculated by productivity of species and susceptibility of fisheries on fish species. In this study, a semi-quantitative method was used to evaluate the risks of exploitation for 20 pelagic fish species by the small-scale longline fisheries in the western North Pacific Ocean. The productivity was estimated based on the ranking (high, median, and low) of seven life history parameters. The susceptibility was calculated by the multiplication of the catchability, selectivity and post-capture mortality. The ERA results indicated the risks of sharks are higher than those of tunas and billfishes, except yellowfin tuna (Thunnus albacares). The shortfin mako shark (Isurus oxyrinchus) and dusky shark (Carcharhinus obscurus) have the highest risk. Other shark species, yellowfin tuna, and sailfish (Istiophorus platypterus) have medium risk. While the striped marlin (Kajikia audax), and albacore tuna (T. alalunga) have the lowest risk. Stock assessment and rigorous management measures such as catch quota and size limit are recommended for the species in high or medium ecological risk and a consistent monitoring management scheme is suggested for those in low ecological risk.
Expression of Hormones, Cytokines, and Antioxidants in Heat-Stressed Subfertile Female Dromedaries
Authors: Zeitoun Moustafa , Derar Derar , Ali Ahmed , Alharbi Yousef
Published: August 2022

The prevailing hot climate imposes heavy burdens on the productivity of the camel, goat, and sheep herds raised in the Gulf desert. Due to the lack of a reliable indicator for the various subfertility statuses in camel females, this study aimed to investigate the expression of inhibin, TGFά, ILß, FSH, sex and metabolic hormones, and antioxidants for the fertility status in camel females. Eighty-two subfertile and five fertile females were admitted to the university clinic with the complaint of repeat breeding with failed conception. The animal’s genital tracts were examined for reproductive soundness. Blood samples were withdrawn for hormonal, cytokines, and antioxidants determinations. Subfertile females were categorized into six groups; endometritis (EN, 28), inactive ovaries (IO, 20), ovarian hydrobursitis (BU, 19), vaginal adhesions (VA, 7), salpingitis (SA, 4), and cervicitis (CE, 4). Results revealed a significant increase in inhibin in all groups compared to control (68.2, 66.4, 61.8. 58.8, 58.3, 55.8, and 36 pg/mL, in CE, VA, IO, BU, EN, SA, and CON, respectively). TGFά, dehydroepiandrosterone (DHEA), and progesterone were not different among groups, whereas IL-ß differed among groups. FSH, estradiol, nitrous oxide, and glutathione were higher in CON compared with other groups. In conclusion, reproductive failures in camel females are reflected in the imbalances of endocrine, cytokines, and antioxidants bio-indicators.