bluuebunny/crossref_metadata_2025 huggingface dataset:
The Polar Lipid Fraction E from Sulfolobus acidocaldarius Can Be Used as Liposomal Drug Stabilizing Agents to Reduce the Leakage of the Antivascular Drug Combretastatin A4 Disodium Phosphate from Tetraether/Diester Hybrid Archaeosomes
Authors: Daswani Varsha P. , Ayesa Umme , Chong Parkson Lee-Gau
Published: December 2021

Liposomes have many advantages as therapeutic capsules over free drugs such as small molecule drugs and nucleic acids. Cholesterol is commonly used as a membrane stabilizing agent in liposomal drugs (e.g., mRNA-lipid nanoparticle COVID-19 vaccines). However, due to the vulnerability of cholesterol to oxidation and the etiological role of cholesterol in many disorders, it is desirable to find an alternative means to stabilize liposomal membranes for drug delivery. In this study, we demonstrated that the polar lipid fraction E (PLFE), which contains exclusively bipolar tetraether macrocyclic lipids, isolated from the thermoacidophilic archaeon S. acidocaldarius can greatly stabilize the liposomal formulation of the anti-vascular drug, combretastatin A4 disodium phosphate (CA4P). Stability was assessed by determining the leakage rate constant k of entrapped CA4P fluorometrically. We found that, at 37 °C, PLFE decreases the k value monotonically from 1.54 × 10−2 s−1 for 100% 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) liposomes to 3.4 × 10−5 s−1 for 100% PLFE archaeosomes, a change of k by two orders of magnitude. The changes in k of CA4P leakage are correlated well with the changes in liposomal CA4P’s cytotoxicity against MCF-7 breast cancer cells. We further showed that the reduction in spontaneous leakage of entrapped CA4P by PLFE can be attributed to the increased membrane surface charge and the increased membrane order and packing tightness in liposomes, as reflected by the zeta potential (−6.83 to −41.1 mV from 0 to 100 mol% PLFE) and diphenylhexatriene (DPH) fluorescence polarization (0.13 to 0.4 from 0 to 100 mol% PLFE) measurements. Moreover, we showed that PLFE slows down CA4P leakage more than cholesterol in POPC liposomes. These results together suggest that PLFE lipids can serve as an effective stabilizing agent for liposomal drugs and could potentially be useful for the optimization of liposomal CA4P for cancer treatment.
Upper Limb Robotic Rehabilitation for Patients with Cervical Spinal Cord Injury: A Comprehensive Review
Authors: Morone Giovanni , de Sire Alessandro , Martino Cinnera Alex , Paci Matteo , Perrero Luca , Invernizzi Marco , Lippi Lorenzo , Agostini Michela , Aprile Irene , Casanova Emanuela , Marino Dario , La Rosa Giuseppe , Bressi Federica , Sterzi Silvia , Giansanti Daniele , Battistini Alberto , Miccinilli Sandra , Filoni Serena , Sicari Monica , Petrozzino Salvatore , Solaro Claudio Marcello , Gargano Stefano , Benanti Paolo , Boldrini Paolo , Bonaiuti Donatella , Castelli Enrico , Draicchio Francesco , Falabella Vincenzo , Galeri Silvia , Gimigliano Francesca , Grigioni Mauro , Mazzoleni Stefano , Mazzon Stefano , Molteni Franco , Petrarca Maurizio , Picelli Alessandro , Gandolfi Marialuisa , Posteraro Federico , Senatore Michele , Turchetti Giuseppe , Straudi Sofia
Published: December 2021

The upper extremities limitation represents one of the essential functional impairments in patients with cervical spinal cord injury. Electromechanics assisted devices and robots are increasingly used in neurorehabilitation to help functional improvement in patients with neurological diseases. This review aimed to systematically report the evidence-based, state-of-art on clinical applications and robotic-assisted arm training (RAT) in motor and functional recovery in subjects affected by cervical spinal cord injury. The present study has been carried out within the framework of the Italian Consensus Conference on “Rehabilitation assisted by robotic and electromechanical devices for persons with disability of neurological origin” (CICERONE). PubMed/MEDLINE, Cochrane Library, and Physiotherapy Evidence Database (PEDro) databases were systematically searched from inception to September 2021. The 10-item PEDro scale assessed the study quality for the RCT and the AMSTAR-2 for the systematic review. Two different authors rated the studies included in this review. If consensus was not achieved after discussion, a third reviewer was interrogated. The five-item Oxford CEBM scale was used to rate the level of evidence. A total of 11 studies were included. The selected studies were: two systematic reviews, two RCTs, one parallel-group controlled trial, one longitudinal intervention study and five case series. One RCT was scored as a high-quality study, while the systematic review was of low quality. RAT was reported as feasible and safe. Initial positive effects of RAT were found for arm function and quality of movement in addition to conventional therapy. The high clinical heterogeneity of treatment programs and the variety of robot devices could severely affect the generalizability of the study results. Therefore, future studies are warranted to standardize the type of intervention and evaluate the role of robotic-assisted training in subjects affected by cervical spinal cord injury.
Biological, Psychological, and Social Determinants of Depression: A Review of Recent Literature
Authors: Remes Olivia , Mendes João Francisco , Templeton Peter
Published: December 2021

Depression is one of the leading causes of disability, and, if left unmanaged, it can increase the risk for suicide. The evidence base on the determinants of depression is fragmented, which makes the interpretation of the results across studies difficult. The objective of this study is to conduct a thorough synthesis of the literature assessing the biological, psychological, and social determinants of depression in order to piece together the puzzle of the key factors that are related to this condition. Titles and abstracts published between 2017 and 2020 were identified in PubMed, as well as Medline, Scopus, and PsycInfo. Key words relating to biological, social, and psychological determinants as well as depression were applied to the databases, and the screening and data charting of the documents took place. We included 470 documents in this literature review. The findings showed that there are a plethora of risk and protective factors (relating to biological, psychological, and social determinants) that are related to depression; these determinants are interlinked and influence depression outcomes through a web of causation. In this paper, we describe and present the vast, fragmented, and complex literature related to this topic. This review may be used to guide practice, public health efforts, policy, and research related to mental health and, specifically, depression.
Neuropsychological Characterization of Aggressive Behavior in Children and Adolescents with CD/ODD and Effects of Single Doses of Medications: The Protocol of the Matrics_WP6-1 Study
Authors: Balia Carla , Carucci Sara , Milone Annarita , Romaniello Roberta , Valente Elena , Donno Federica , Montesanto Annarita , Brovedani Paola , Masi Gabriele , Glennon Jeffrey C. , Coghill David , Zuddas Alessandro ,   the MATRICS Consortium
Published: December 2021

Aggressive behaviors and disruptive/conduct disorders are some of the commonest reasons for referral to youth mental health services; nevertheless, the efficacy of therapeutic interventions in real-world clinical practice remains unclear. In order to define more appropriate targets for innovative pharmacological therapies for disruptive/conduct disorders, the European Commission within the Seventh Framework Programme (FP7) funded the MATRICS project (Multidisciplinary Approaches to Translational Research in Conduct Syndromes) to identify neural, genetic, and molecular factors underpinning the pathogenesis of aggression/antisocial behavior in preclinical models and clinical samples. Within the program, a multicentre case-control study, followed by a single-blind, placebo-controlled, cross-over, randomized acute single-dose medication challenge, was conducted at two Italian sites. Aggressive children and adolescents with conduct disorder (CD) or oppositional defiant disorder (ODD) were compared to the same age (10–17 y) typically developing controls (TDC) on a neuropsychological tasks battery that included both “cold” (e.g., inhibitory control, decision making) and “hot” executive functions (e.g., moral judgment, emotion processing, risk assessment). Selected autonomic measures (heart rate variability, skin conductance, salivary cortisol) were recorded before/during/after neuropsychological testing sessions. The acute response to different drugs (methylphenidate/atomoxetine, risperidone/aripiprazole, or placebo) was also examined in the ODD/CD cohort in order to identify potential neuropsychological/physiological mechanisms underlying aggression. The paper describes the protocol of the clinical MATRICS WP6-1 study, its rationale, the specific outcome measures, and their implications for a precision medicine approach.
Molecular Classification and Therapeutic Targets in Ependymoma
Authors: Larrew Thomas , Saway Brian Fabian , Lowe Stephen R. , Olar Adriana
Published: December 2021

Ependymoma is a biologically diverse tumor wherein molecular classification has superseded traditional histological grading based on its superior ability to characterize behavior, prognosis, and possible targeted therapies. The current, updated molecular classification of ependymoma consists of ten distinct subgroups spread evenly among the spinal, infratentorial, and supratentorial compartments, each with its own distinct clinical and molecular characteristics. In this review, the history, histopathology, standard of care, prognosis, oncogenic drivers, and hypothesized molecular targets for all subgroups of ependymoma are explored. This review emphasizes that despite the varied behavior of the ependymoma subgroups, it remains clear that research must be performed to further elucidate molecular targets for these tumors. Although not all ependymoma subgroups are oncologically aggressive, development of targeted therapies is essential, particularly for cases where surgical resection is not an option without causing significant morbidity. The development of molecular therapies must rely on building upon our current understanding of ependymoma oncogenesis, as well as cultivating transfer of knowledge based on malignancies with similar genomic alterations.
Cancer Therapy Targeting CD47/SIRPα
Authors: Dizman Nazli , Buchbinder Elizabeth I.
Published: December 2021

In the past decade, the field of cancer immunotherapy has rapidly advanced, establishing a crucial role for immune checkpoint blockers in the treatment of a variety of cancer types. In parallel with these remarkable clinical developments, further efforts have focused on ways of unleashing adaptive immune responses against cancer. CD47, a cell surface molecule overexpressed by several cancer types that facilitates immune escape from macrophages, dendritic cells and natural killer cells, and its ligand SIRPα, have emerged as potential therapeutic targets. A number of agents directed to CD47/SIRPα have been developed and demonstrated preclinical activity. Early phase clinical trials are investigating CD47/SIRPα directed agents with available data, suggesting safety and preliminary activity. Herein, we provide an overview of the mechanistic rationale of targeting CD47/SIRPα axis and associated clinical evidence.
Radioiodine Refractory Follicular Thyroid Cancer and Surgery for Cervical Relapse
Authors: Chiapponi Costanza , Hartmann Milan J. M. , Schmidt Matthias , Faust Michael , Schultheis Anne M. , Bruns Christiane J. , Alakus Hakan
Published: December 2021

Compared to its more common counterpart papillary thyroid cancer (PTC), follicular thyroid cancer (FTC) has a less favorable outcome, due to its higher incidence of distant metastases and advanced stages at diagnosis. Despite radioiodine (RAI) avidity, metastatic FTC often progresses after radioiodine treatment (RAIT). We aimed at evaluating the indications and outcomes of surgery for cervical relapse of radioiodine refractory FTC. Patients receiving RAIT between 2005 and 2015 at the University Hospital of Cologne, Germany, were screened. Patients with FTC were identified. Demographics, clinic-pathologic characteristics, treatment, and outcome of patients diagnosed with RAI refractory FTC, who underwent cervical surgery in the course of disease, were analyzed. FTC accounted for 8.8% of all thyroid carcinomas undergoing RAIT. In 35.2% of FTC patients, disease persisted or recurred despite a cumulative mean RAI activity of 18.7 GBq ± 11.6 (follow-up 83.5 ± 56.7 months). Distant metastases were diagnosed in 75% of these patients, as bone (57.6%), lung (54.6%), and liver metastases (12.1%). Cervical relapse occurred in 63.6% of these patients and was treated in 57.1% with surgery with, and without, external beam radiation therapy (EBRT). Despite surgery and EBRT, in 75% of patients, cervical relapse recurred again. In conclusion, surgery for cervical radioiodine refractory FTC relapse is often performed in metastatic setting. With and without EBRT, cure is rare, although metastases can appear radioiodine avid. Early biological marker and systemic treatments for these patients are still needed.
Locus-Specific Methylation of GSTP1, RNF219, and KIAA1539 Genes with Single Molecule Resolution in Cell-Free DNA from Healthy Donors and Prostate Tumor Patients: Application in Diagnostics
Authors: Bryzgunova Olga , Bondar Anna , Ruzankin Pavel , Laktionov Petr , Tarasenko Anton , Kurilshikov Alexander , Epifanov Rostislav , Zaripov Marat , Kabilov Marsel , Laktionov Pavel
Published: December 2021

The locus-specific methylation of three genes (GSTP1, RNF219, and KIAA1539 (also known as FAM214B)) in the blood plasma cell-free DNA (cfDNA) of 20 patients with prostate cancer (PCa), 18 healthy donors (HDs), and 17 patients with benign prostatic hyperplasia (BPH) was studied via the MiSeq platform. The methylation status of two CpGs within the same loci were used as the diagnostic feature for discriminating the patient groups. Many variables had good diagnostic characteristics, e.g., each of the variables GSTP1.C3.C9, GSTP1.C9, and GSTP1.C9.T17 demonstrated an 80% sensitivity at a 100% specificity for PCa patients vs. the others comparison. The analysis of RNF219 gene loci methylation allowed discriminating BPH patients with absolute sensitivity and specificity. The data on the methylation of the genes GSTP1 and RNF219 allowed discriminating PCa patients, as well as HDs, with absolute sensitivity and specificity. Thus, the data on the locus-specific methylation of cfDNA (with single-molecule resolution) combined with a diagnostic approach considering the simultaneous methylation of several CpGs in one locus enabled the discrimination of HD, BPH, and PCa patients.
Pyroptosis: Mechanisms and Links with Fibrosis
Authors: Song Zihao , Gong Quan , Guo Jiawei
Published: December 2021

Fibrosis is responsible for approximately 45% of deaths in the industrialized world and has been a major global healthcare burden. Excessive fibrosis is the primary cause of organ failure. However, there are currently no approved drugs available for the prevention or treatment of fibrosis-related diseases. It has become evident that fibrosis is characterized by inflammation. In a large number of studies of various organs in mice and humans, pyroptosis has been found to play a significant role in fibrosis. Pyroptosis is a form of programmed cell death mediated by the N-terminal fragment of cysteinyl aspartate-specific proteinase (caspase)-1-cleaved gasdermin D (GSDMD, producing GSDMD-N) that gives rise to inflammation via the release of some proinflammatory cytokines, including IL-1β, IL-18 and HMGB1. These cytokines can initiate the activation of fibroblasts. Inflammasomes, an important factor upstream of GSDMD, can activate caspase-1 to trigger the maturation of IL-1β and IL-18. Moreover, the inhibition of inflammasomes, proinflammatory cytokines and GSDMD can prevent the progression of fibrosis. This review summarizes the growing evidence indicating that pyroptosis triggers fibrosis, and highlights potential novel targets for antifibrotic therapies.
Effect of Pulsing Configuration and Magnetic Balance Degree on Mechanical Properties of CrN Coatings Deposited by Bipolar-HiPIMS onto Floating Substrate
Authors: Tiron Vasile , Ciolan Mihai , Bulai Georgiana , Cristea Daniel , Velicu Ioana-Laura
Published: December 2021

Despite its great potential for thin films deposition and technological applications, the HiPIMS technology has its own limitations including the control of ion energy and flux towards the substrate when coping with the deposition of electrical insulating films and/or the deposition onto insulating/electrically grounded substrates. The bipolar-HiPIMS has been recently developed as a strategy to accelerate the plasma ions towards a growing film maintained at ground potential. In this work, the benefits of bipolar-HiPIMS deposition onto floating or nonconductive substrates are explored. The effect of bipolar-HIPIMS pulsing configuration, magnetic balance-unbalance degree, and substrate’s condition on plasma characteristics, microstructure evolution, and mechanical properties of CrN coatings was investigated. During the deposition with a balanced magnetron configuration, a significant ion bombardment effect was detected when short negative pulses and relative long positive pulses were used. XRD analysis and AFM observations revealed significant microstructural changes by increasing the positive pulse duration, which results in an increase in hardness from 7.3 to 16.2 GPa, during deposition on grounded substrates, and from 4.9 to 9.4 GPa during the deposition on floating substrates. The discrepancies between the hardness values of the films deposited on floating substrates and those of the films deposited on grounded substrates become smaller/larger when a type I/type II unbalanced magnetron configuration is used. Their hardness ratio was found to be 0.887, in the first case, and 0.393, in the second one. Advanced application-tailored coatings can be deposited onto floating substrates by using the bipolar-HiPIMS technology if short negative pulses, relative long positive pulses together with type I unbalanced magnetron are concomitantly used.
Endangered Apes—Can Their Behaviors Be Used to Index Fear and Disturbance in Anthropogenic Landscapes?
Authors: Reisland Melissa A. , Malone Nicholas , Lambert Joanna E.
Published: December 2021

Behaviors exhibited by prey species towards predators (including humans) can reduce feeding time and increase time spent in vigilance, thereby impacting animal condition and ultimately limit populations, even when actual mortality from predation is low. Here, we test whether behavioral profiles in an endangered ape, Javan gibbons (Hylobates moloch), correspond to varying degrees of human disturbance in a human-impacted sacred forest, Cagar Alam Leuweung Sancang, West Java. Data were collected August 2010–July 2011. Although all groups reacted differently to human presence, overall, gibbons responded by reducing time spent on conspicuous behavior (e.g., vocalizing, feeding, traveling, and social interactions) as the number of humans in the area increased or distance to the nearest human decreased. In addition, gibbon responses to encountering humans were also more like their responses to encountering potential predators than they were to encountering monkeys or other gibbons. These results support the hypothesis that as human presence and encounter rates increase, gibbons alter their behavior in ways consistent with anti-predator behaviors. Assessing how this endangered species responds to human presence is a vital part of their ultimate conservation.
Intra- and Inter-Annual Growth Patterns of a Mixed Pine-Oak Forest under Mediterranean Climate
Authors: Martínez-Sancho Elisabet , Gutiérrez Emilia , Valeriano Cristina , Ribas Montse , Popkova Margarita I. , Shishov Vladimir V. , Dorado-Liñán Isabel
Published: December 2021

Temperature and precipitation variability throughout the year control the intra-annual dynamics of tree-ring formation. Physiological adaptation of trees to climate change is among the key issues to better understand and predict future forest performance and composition. In this study, we investigated the species’ coexistence and performance of Scots pine and pubescent oak growing in a mixed sub-Mediterranean forest in the northeast of the Iberian Peninsula. We assessed intra-annual cumulative growth patterns derived from band dendrometers during four consecutive growing seasons and long-term changes in basal area increment for the period 1950–2014. Our results revealed that Scots pine followed an intra-annual bimodal growth pattern. Scots pine growth was mainly limited by water availability at intra-annual, interannual and decadal time scales, which resulted in a negative long-term growth trend. Conversely, oak displayed a unimodal growth pattern, which was less climatically constrained. A significant increase in basal area of oak denotes an overall better potential acclimation to prevailing climatic conditions at the expenses of a higher risk of physiological failure during extreme climate events.
Flowers Characteristics of Selected Species of Lime-Tree (Tilia spp.) in Terms of miRNA-Based Markers Activity, Mannose Expression and Biological Compounds Content
Authors: Ražná Katarína , Žiarovská Jana , Ivanišová Eva , Urbanová Lucia , Harenčár Ľubomír , Kováčik Adam , Kučka Matúš , Hrubík Pavel
Published: December 2021

The significant healing effect of Tilia platyphyllos Scop. and Tilia cordata Mill. flowers are well known. However, the flowers of Tilia tomentosa Moench. are not suitable for harvest due to their toxic effects. To investigate the diverse background of this effect, we applied a functional miRNA-based marker, mannose expression analysis and determined the content of bioactive compounds. Out of nine tested markers, three (miR160, miR167 and miR408) provided reproducible miRNA-based loci and two of them (miR160 and miR398) enabled the acquisition of fingerprinting specific to flower and leaf samples of T. platyphyllos and T. tomentosa. The most pronounced profiling was specific for miR408 marker, the function of which is connected to plant defense and adaptation mechanisms. We confirmed the suitability of microRNA-based markers for polymorphism determination of flowers of selected species of lime-tree. The highest values of antioxidant activity, flavonoids, total polyphenols and phenolic acids content have been reached in silver linden flowers. When comparing the transcription activity of mannose in flowers, more than 30 times higher levels of mannose transcripts for the silver linden flowers was observed.
Cognitive-Based Interventions Break Gender Stereotypes in Kindergarten Children
Authors: Chung Yi , Huang Hsin-Hui
Published: December 2021

Despite the growing recognition of gender equality worldwide, plausible strategies that reduce young children’s gender stereotypes remain limited. Cognitive-based interventions have been widely used in school settings and have been suggested to play important roles in children’s gender stereotyping and in their processing of counter-stereotypic information. We aimed to determine whether exposure to counter-stereotypical information could break gender stereotypes in kindergarten children. Fifty-four children (61–79 months old) from two public kindergarten classes in northern Taiwan participated in this study. One of the two classes was randomly selected as the experimental group (n = 28), and the other was the control group (n = 26). The experimental group consisted of a gender equality curriculum including script relationship training for two months, while the control group continued their regular curriculum. The picture classification task (PCT) was measured before and after the intervention to assess gender stereotypes. Before interventions, 87.50% of the children chose a gender stereotypic relationship, while 12.50% chose script/other relationships in PCT. After the interventions, the gender stereotypic relationship dropped to 73.22% in the experimental group. Children in the control group were more likely to maintain their gender stereotypic relationship choices in PCTs. Our findings suggest that cognitive-based interventions, such as a gender equality curriculum, have the potential to break gender stereotypes in kindergarten children.
Predictive Factors of Short-Term Related Musculoskeletal Pain in the Automotive Industry
Authors: Assunção Ana , Moniz-Pereira Vera , Fujão Carlos , Bernardes Sarah , Veloso António P. , Carnide Filomena
Published: December 2021

To determine the short-term associations between biomechanical risk factors and musculoskeletal symptoms in the upper limbs and low back in an automotive company, a longitudinal study with a follow-up of 4 days was conducted in a sample of 228 workers of the assembly and paint areas. Data were analyzed using generalized estimating equations, calculating the crude and adjusted model for age, sex, seniority, and intensity of pain at baseline. The interactions found were the same for both models. Workers were divided in low-risk and high-risk group for posture, force, exposure, percentage of cycle time with the arm at/above shoulder level, and with the trunk flexed or/and strongly flexed. The predictive factors showed by time × group effect were found between pain intensity on the left shoulder for posture (β = 0.221, p < 0.001), percentage of time with the trunk flexed (β = 0.136, p = 0.030) and overall exposure (β = 0.140, p = 0.013). A time × group interactions were observed, namely between neck pain and posture (β = 0.218, p = 0.005) and right wrist and force (β = 0.107, p = 0.044). Workers in the high-risk group were more prone to report unfavorable effects on their self-reported musculoskeletal pain, across a workweek when exposed to specific risk factor, being posture important to neck, right wrist and left shoulder pain.
Family Caregivers of People with Dementia Have Poor Sleep Quality: A Nationwide Population-Based Study
Authors: Song Min Ji , Kim Ji Hyun
Published: December 2021

Previous studies have documented cognitive impairments, psychological stress, and depressive symptoms in family caregivers of people with dementia (PWD), which could be attributed to their sleep disturbances. Notwithstanding the increasing recognition of poor sleep quality and sleep disturbances in family caregivers of PWD, their association has not been tested yet using population-representative samples. We conducted a retrospective, cross-sectional study using population-based data from the 2018 Korean Community Health Survey. Sociodemographic, mental health-related, and physical health-related variables as well as sleep quality evaluated by the Pittsburgh Sleep Quality Index (PSQI) were compared between 2537 cohabitating caregivers of PWD, 8864 noncohabitating caregivers of PWD, and 190,278 non-caregivers. Two sets of multivariable logistic regressions were conducted to examine the associations between dementia caregiving and poor sleep quality in cohabitating and noncohabitating caregivers versus noncaregivers. Both cohabitating and noncohabitating caregivers had higher global PSQI scores and higher prevalence of poor sleep quality (PSQI score > 5) than did noncaregivers. Multivariable logistic regressions adjusted for potential confounders revealed that cohabitating caregivers (odds ratio (OR) 1.26, 95% confidence interval (CI) 1.15–1.38) and noncohabitating caregivers (OR 1.15, CI 1.10–1.21) were significantly associated with poor sleep quality. Our results showed that both cohabitating and noncohabitating caregivers of PWD experienced overall poorer sleep quality compared to noncaregivers, indicating the deleterious effect of dementia caregiving on sleep quality, regardless of living arrangements. Given the high prevalence of poor sleep quality in family caregivers of PWD and the increasing awareness of the serious health consequences of poor-quality sleep, physicians should consider active sleep interventions to promote health and wellbeing not only for the dementia patients but also for family caregivers.
Spatiotemporal Evolution and Influencing Factors of Carbon Sink Dynamics at County Scale: A Case Study of Shaanxi Province, China
Authors: Liu Shuohua , Zhang Xiao , Zhou Yifan , Yao Shunbo
Published: December 2021

To explore the spatiotemporal evolution of carbon sinks in Shaanxi Province, and their impact mechanisms, this study used panel data from 107 counties (districts) in Shaanxi Province from 2000 to 2017. First, we conducted spatial distribution directional analysis and exploratory spatial data analysis (ESDA). Then, we constructed a geographic spatial weight matrix and used the spatial panel Durbin model to analyze the driving factors of carbon sink changes in Shaanxi Province, from the perspective of spatial effects. The results showed that: (1) The temporal evolution of carbon sinks during the study period showed an overall upward trend, but the carbon sinks of counties (districts) differed greatly, and the center of gravity of carbon sinks, as a whole, showed the characteristics of “south to north” migration. (2) The carbon sinks of Shaanxi Province have a significant positive global spatial autocorrelation in geographic space. The local spatial pattern was characterized by low-value agglomeration (low-low cluster) and high-value agglomeration (high-high cluster), supplemented by high-value bulge (high-low outlier) and low-value collapse (low-high outlier). (3) The result of the spatial measurement model proved that the spatial Durbin model, with dual fixed effects of time and space, should be selected. In the model results, factors such as population, per capita gross domestic product (GDP), local government general budget expenditure, and local government general budget revenue all reflect strong spatial spillover effects. Accordingly, in the process of promoting “carbon neutrality”, the government needs to comprehensively consider the existence of spatial spillover effects between neighboring counties (districts), and strengthen the linkage-management and control roles of counties (districts) in increasing carbon sinks.
Identification of Policies Based on Assessment-Optimization Model to Confront Vulnerable Resources System with Large Population Scale in a Big City
Authors: Zeng Xueting , Xiang Hua , Liu Jia , Xue Yong , Zhu Jinxin , Xu Yuqian
Published: December 2021

The conflict between excessive population development and vulnerable resource (including water, food, and energy resources) capacity influenced by multiple uncertainties can increase the difficulty of decision making in a big city with large population scale. In this study, an adaptive population and water–food–energy (WFE) management framework (APRF) incorporating vulnerability assessment, uncertainty analysis, and systemic optimization methods is developed for optimizing the relationship between population development and WFE management (P-WFE) under combined policies. In the APRF, the vulnerability of WFE was calculated by an entropy-based driver–pressure–state–response (E-DPSR) model to reflect the exposure, sensitivity, and adaptability caused by population growth, economic development, and resource governance. Meanwhile, a scenario-based dynamic fuzzy model with Hurwicz criterion (SDFH) is proposed for not only optimizing the relationship of P-WFE with uncertain information expressed as possibility and probability distributions, but also reflecting the risk preference of policymakers with an elected manner. The developed APRF is applied to a real case study of Beijing city, which has characteristics of a large population scale and resource deficit. The results of WFE shortages and population adjustments were obtained to identify an optimized P-WEF plan under various policies, to support the adjustment of the current policy in Beijing city. Meanwhile, the results associated with resource vulnerability and benefit analysis were analyzed for improving the robustness of policy generation.
Nurse Education and Mathematical Competency: Implementation of an Online, Self-Directed, Prerequisite Model
Authors: Jarvis Daniel H. , McCullough Karey D. , McParland Tammie R.
Published: December 2021

Mathematical competency in the profession of nursing has increasingly become a central focus as more nursing students appear to struggle with basic concepts of arithmetic, mental estimation, and critical reasoning. This paper highlights how one School of Nursing in Ontario, Canada implemented a Dosage Calculation Competency Test model which involved an online, self-directed, prerequisite approach to improve student mathematical competency and confidence. The purpose of this research case study was to document, through shared participant perceptions, the creation, implementation, and subsequent modifications to a Dosage Calculation Competency Test model in light of student needs and advances in online learning and assessment. The research design combined a quantitative survey of Year 1–4 nursing students, followed by a series of qualitative, semi-structured interviews with nursing students and program instructors. The study took place within a School of Nursing undergraduate program in Ontario, Canada. Forty-four participants, including students from all four years of the nursing program, completed the survey, followed by individual interviews with nine students and six faculty instructors. Survey (the open-response items) and interview data were analyzed thematically using ATLAS.ti (ATLAS.ti, Berlin, Germany). The authors recount the new DCCT model’s development, implementation, and subsequent modifications and further discuss student/instructor perceptions of learning types, math confidence, and competency. The paper concludes with a series of seven key recommendations for nursing programs.
Barriers to Clinician Implementation of Parent-Child Interaction Therapy (PCIT) in New Zealand and Australia: What Role for Time-Out?
Authors: Woodfield Melanie J. , Cargo Tania , Merry Sally N. , Hetrick Sarah E.
Published: December 2021

Background: Parent-Child Interaction Therapy (PCIT) is an effective parent training approach for a commonly occurring and disabling condition, namely conduct problems in young children. Yet, despite ongoing efforts to train clinicians in PCIT, the intervention is not widely available in New Zealand and Australia. Methods: We undertook a cross-sectional online survey of clinicians in New Zealand and Australia who had completed at least the 40-h initial PCIT training, to understand the barriers they encountered in their implementation efforts, and the extent to which attitudes toward time-out influenced implementation. The overall response rate was 47.5% (NZ: 60%; Australia: 31.4%). Results: Responses suggested that participants generally viewed PCIT as both acceptable and effective. Australian participants reported seeing significantly more clients for PCIT per week than those in NZ (Medians 0 and 2, respectively; χ2(1) = 14.08, p < 0.001) and tended to view PCIT as more effective in treating disruptive and oppositional behaviour (95% CI: −0.70, −0.13, p = 0.005). Participants currently seeing PCIT clients described it as more enjoyable to implement than those not using PCIT (95% CI: −0.85, −0.10, p = 0.01). Thirty-eight percent of participants indicated that they adapt or tailor the standardised protocol, primarily by adding in content relating to emotion regulation, and removing content relating to time-out. Participants generally felt that they had fewer skills, less knowledge, and less confidence relating to the Parent-Directed Interaction phase of PCIT (which involves time-out), compared with the Child-Directed Interaction phase. Conclusion: While we had hypothesised that time-out represented an intra-intervention component that detracted from implementation success, results suggested that clinician concern over the use of time-out was present but not prominent. Rather, the lack of access to suitable equipment (i.e., one-way mirror and ear-piece) and difficulties associated with clients attending clinic-based sessions were barriers most commonly reported by clinicians. We suggest that future research might consider whether and how PCIT might be “re-implemented” by already-trained clinicians, moving beyond simply training more clinicians in the approach.
Circulating Microvesicle-Associated Inducible Nitric Oxide Synthase Is a Novel Therapeutic Target to Treat Sepsis: Current Status and Future Considerations
Authors: Webber Robert , Sweet Richard , Webber Douglas
Published: December 2021

To determine whether mitigating the harmful effects of circulating microvesicle-associated inducible nitric oxide (MV-A iNOS) in vivo increases the survival of challenged mice in three different mouse models of sepsis, the ability of anti-MV-A iNOS monoclonal antibodies (mAbs) to rescue challenged mice was assessed using three different mouse models of sepsis. The vivarium of a research laboratory Balb/c mice were challenged with an LD80 dose of either lipopolysaccharide (LPS/endotoxin), TNFα, or MV-A iNOS and then treated at various times after the challenge with saline as control or with an anti-MV-A iNOS mAb as a potential immunotherapeutic to treat sepsis. Each group of mice was checked daily for survivors, and Kaplan–Meier survival curves were constructed. Five different murine anti-MV-A iNOS mAbs from our panel of 24 murine anti-MV-A iNOS mAbs were found to rescue some of the challenged mice. All five murine mAbs were used to genetically engineer humanized anti-MV-A iNOS mAbs by inserting the murine complementarity-determining regions (CDRs) into a human IgG1,kappa scaffold and expressing the humanized mAbs in CHO cells. Three humanized anti-MV-A iNOS mAbs were effective at rescuing mice from sepsis in three different animal models of sepsis. The effectiveness of the treatment was both time- and dose-dependent. Humanized anti-MV-A iNOS rHJ mAb could rescue up to 80% of the challenged animals if administered early and at a high dose. Our conclusions are that MV-A iNOS is a novel therapeutic target to treat sepsis; anti-MV-A iNOS mAbs can mitigate the harmful effects of MV-A iNOS; the neutralizing mAb’s efficacy is both time- and dose-dependent; and a specifically targeted immunotherapeutic for MV-A iNOS could potentially save tens of thousands of lives annually and could result in improved antibiotic stewardship.
Hypoxia Increases Nitric Oxide-Dependent Inhibition of Angiogenic Growth
Authors: Arce Cristina , Vicente Diana , Monto Fermí , González Laura , Nuñez Cristina , Victor Víctor M. , Jiménez-Altayó Francesc , D’Ocon Pilar
Published: December 2021

Nitric oxide (NO) is a proangiogenic factor acting through the soluble guanylate cyclase (sGC) pathway. However, angiogenic growth increases energy demand, which may be hampered by NO inhibition of cytochrome c oxidase (CcO). Then, NO activity would be the balanced result of sGC activation (pro-angiogenic) and CcO inhibition (anti-angiogenic). NO activity in a rat and eNOS−/− mice aortic ring angiogenic model and in a tube formation assay (human aortic endothelial cells) were analyzed in parallel with mitochondrial O2 consumption. Studies were performed with NO donor (DETA-NO), sGC inhibitor (ODQ), and NOS or nNOS inhibitors (L-NAME or SMTC, respectively). Experiments were performed under different O2 concentrations (0–21%). Key findings were: (i) eNOS-derived NO inhibits angiogenic growth by a mechanism independent on sGC pathway and related to inhibition of mitochondrial O2 consumption; (ii) NO inhibition of the angiogenic growth is more evident in hypoxic vessels; (iii) in the absence of eNOS-derived NO, the modulation of angiogenic growth, related to hypoxia, disappears. Therefore, NO, but not lower O2 levels, decreases the angiogenic response in hypoxia through competitive inhibition of CcO. This anti-angiogenic activity could be a promising target to impair pathological angiogenesis in hypoxic conditions, as it occurs in tumors or ischemic diseases.
Insects: Functional Morphology, Biomechanics and Biomimetics
Authors: Rajabi Hamed , Wu Jianing , Gorb Stanislav
Published: December 2021

Insects are the most diverse animal taxon, both in terms of the number of species and the number of individuals [...]
Envisaging Antifungal Potential of Histatin 5: A Physiological Salivary Peptide
Authors: Sharma Pratibha , Chaudhary Mehak , Khanna Garima , Rishi Praveen , Kaur Indu Pal
Published: December 2021

Fungi are reported to cause a range of superficial to invasive human infections. These often result in high morbidity and at times mortality. Conventional antifungal agents though effective invariably exhibit drug interactions, treatment-related toxicity, and fail to elicit significant effect, thus indicating a need to look for suitable alternatives. Fungi thrive in humid, nutrient-enriched areas. Such an environment is well-supported by the oral cavity. Despite this, there is a relatively low incidence of severe oral and periodontal fungal infections, attributed to the presence of antimicrobial peptides hosted by saliva, viz. histatin 5 (Hstn 5). It displays fungicidal activity against a variety of fungi including Candida albicans, Candida glabrata, Candida krusei, Cryptococcus neoformans, and unicellular yeast-like Saccharomyces cerevisiae. Candida albicans alone accounts for about 70% of all global fungal infections including periodontal disease. This review intends to discuss the scope of Hstn 5 as a novel recourse for the control of fungal infections.
Detection of Archaeological Surface Ceramics Using Deep Learning Image-Based Methods and Very High-Resolution UAV Imageries
Authors: Agapiou Athos , Vionis Athanasios , Papantoniou Giorgos
Published: December 2021

Mapping surface ceramics through systematic pedestrian archaeological survey is considered a consistent method to recover the cultural biography of sites within a micro-region. Archaeologists nowadays conduct surface survey equipped with navigation devices counting, documenting, and collecting surface archaeological potsherds within a set of plotted grids. Recent advancements in unmanned aerial vehicles (UAVs) and image processing analysis can be utilised to support such surface archaeological investigations. In this study, we have implemented two different artificial intelligence image processing methods over two areas of interest near the present-day village of Kophinou in Cyprus, in the Xeros River valley. We have applied a random forest classifier through the Google Earth Engine big data cloud platform and a Single Shot Detector neural network in the ArcGIS Pro environment. For the first case study, the detection was based on red–green–blue (RGB) high-resolution orthophotos. In contrast, a multispectral camera covering both the visible and the near-infrared parts of the spectrum was used in the second area of investigation. The overall results indicate that such an approach can be used in the future as part of ongoing archaeological pedestrian surveys to detect scattered potsherds in areas of archaeological interest, even if pottery shares a very high spectral similarity with the surface.
Analysis of Exhaust Gas Content for Selected Biofuel-Powered Combustion Engines with Simultaneous Modification of Their Controllers
Authors: Markiewicz Marietta , Pająk Michał , Muślewski Łukasz
Published: December 2021

The use of renewable resources for powering self-ignition engines in European Union countries involves a high demand for renewable energy which is not accompanied by the development of its production infrastructure. The application of biofuel in vehicle powering is supposed to provide reductions in greenhouse gas emissions and an increase in the share of renewable energy resources in the total energy consumption. The study includes the analyses of power unit exhaust components, such as oxygen, carbon monoxide, nitric oxides, carbonizers, carbon dioxide and a quantity of exhaust particles contained in exhaust gases. Tests using an exhaust gas analyzer and a vapor analyzer were conducted. Three high-pressure engines, characterized by direct fuel injection, were tested. The vehicle computer software adjustments included increasing the fuel dose and the air load. Mixtures of diesel oil and fatty acid methyl esters were used in the tests. Based on the results, a statistical analysis was performed and an assessment model was developed to understand the functioning of the research objects fueled with these mixtures, with simultaneous software changes in the vehicle computers. On the basis of the conducted analysis, it was found that only 30% of fatty acid methyl ester additives to diesel oil reduced the performance parameters of the drive units.
Effect of Al2TiO5 Content and Sintering Temperature on the Microstructure and Residual Stress of Al2O3–Al2TiO5 Ceramic Composites
Authors: Fan Kunyang , Jiang Wenhuang , Ruiz-Hervias Jesús , Baudín Carmen , Feng Wei , Zhou Haibin , Bueno Salvador , Yao Pingping
Published: December 2021

A series of Al2O3–Al2TiO5 ceramic composites with different Al2TiO5 contents (10 and 40 vol.%) fabricated at different sintering temperatures (1450 and 1550 °C) was studied in the present work. The microstructure, crystallite structure, and through-thickness residual stress of these composites were investigated by scanning electron microscopy, X-ray diffraction, time-of-flight neutron diffraction, and Rietveld analysis. Lattice parameter variations and individual peak shifts were analyzed to calculate the mean phase stresses in the Al2O3 matrix and Al2TiO5 particulates as well as the peak-specific residual stresses for different hkl reflections of each phase. The results showed that the microstructure of the composites was affected by the Al2TiO5 content and sintering temperature. Moreover, as the Al2TiO5 grain size increased, microcracking occurred, resulting in decreased flexure strength. The sintering temperatures at 1450 and 1550 °C ensured the complete formation of Al2TiO5 during the reaction sintering and the subsequent cooling of Al2O3–Al2TiO5 composites. Some decomposition of AT occurred at the sintering temperature of 1550 °C. The mean phase residual stresses in Al2TiO5 particulates are tensile, and those in the Al2O3 matrix are compressive, with virtually flat through-thickness residual stress profiles in bulk samples. Owing to the thermal expansion anisotropy in the individual phase, the sign and magnitude of peak-specific residual stress values highly depend on individual hkl reflection. Both mean phase and peak-specific residual stresses were found to be dependent on the Al2TiO5 content and sintering temperature of Al2O3–Al2TiO5 composites, since the different developed microstructures can produce stress-relief microcracks. The present work is beneficial for developing Al2O3–Al2TiO5 composites with controlled microstructure and residual stress, which are crucial for achieving the desired thermal and mechanical properties.