bluuebunny/crossref_metadata_2025 huggingface dataset:
PRODUÇÃO DE LACASE DE Pycnoporus sanguineus EM MEIO DE CULTIVO À BASE DE MELAÇO SOJA
Authors: Fabrini Fabricia Flores , Avelino Katielle Vieira , Marim Renan Alberto , Cardoso Bruna Karen , Colauto Giani Andrea Linde , Colauto Nelson Barros , Valle Juliana Silveira do
Published: May 2017

Lacases são polifenol oxidases que utilizam a capacidade redox de íons cobre para reduzir oxigênio a água e oxidar um substrato fenólico. A síntese e secreção de lacases de basidiomicetos dependem de vários fatores como os nutrientes presentes no meio de cultura. Visando à produção de lacase, Pycnoporus sanguineus foi cultivado em meio contendo melaço de soja como única fonte de carbono, ureia como fonte de nitrogênio suplementar em diferentes concentrações (0,6; 1,2; 2,4; 4,8 e 9,6 g/L de nitrogênio) e diferentes concentrações de CuSO4 (0, 150, 200, 250 e 300 µM). O extrato enzimático produzido nas melhores condições de cultivo foi utilizado para a descoloração dos corantes remazol azul brilhante R (antraquinona), amarelo 145, preto 5, vermelho 195 (azo) e verde malaquita (trifenilmetano). As concentrações de nitrogênio não afetaram a produção de lacase, exceto a maior concentração (9,6 g/L) que reduziu a atividade enzimática. A adição de cobre ao meio de cultivo (150 µM) aumentou a atividade de lacase em 112%. A maior atividade de lacase (~34300 U/L) promoveu a descoloração dos corantes remazol azul brilhante R (67,5%) e verde malaquita (28,3%) em 24h, sendo os corantes azo descoloridos apenas parcialmente. Concluiu-se que o melaço de soja é um resíduo agroindustrial adequado para produção de lacase de P. sanguineus com potencial para degradação de corantes.
PERFIL SOCIOECONÔMICO DOS MARISQUEIROS E CONDIÇÕES HIGIÊNICAS ADOTADAS NA CADEIA PRODUTIVA DE OSTRA (MOLLUSCA, BIVALVIA)
Authors: Ribeiro Eliane Braga , Bastos Luciana Da Silva , Almeida Zafira Da Silva de , Neta Raimunda Nonata Fortes Carvalho , Costa Francisca Neide
Published: May 2017

O consumo de ostras na forma in natura exige muito cuidado e práticas higiênicas e sanitárias corretas pelos marisqueiros e demais manipuladores desse alimento durante o processo de beneficiamento à comercialização, para que não haja contaminação por microrganismos patogênicos e deteriorantes. O presente estudo teve como objetivo avaliar o perfil socioeconômico de marisqueiros e as condições higiênicas e sanitárias praticadas na cadeia produtiva de ostras do gênero Crassostrea desde a extração até a comercialização. No período de janeiro a abril de 2014, por meio da técnica de amostragem “Bola de Neve” foram selecionados 40 marisqueiros na Ilha de São Luís - MA, os quais foram entrevistados utilizando-se questionário semiestruturado. De acordo com os resultados, a maioria dos marisqueiros, possui ensino fundamental incompleto, faixa etária entre 21 e 30 anos e encontram-se em união estável ou casados. Parte das ostras são extraídas em bancos naturais na área de estudo, enquanto outra parte é proveniente de atravessadores oriundos de outros municípios maranhenses.Após a aquisição, pode demorar até três dias para serem comercializadas. Concluiu-se que as condições higiênicos sanitárias adotadas na atividade de extração de ostras até a comercialização são insatisfatórias, indicando falta de boas práticas em todas as etapas ao longo do processo de produção, o que pode representar riscos à saúde da população apreciadora do consumo in natura desse bivalve. Além disso, indica a necessidade de fiscalização dessa atividade, já que os organismos são transportados de forma inadequada e passam muito tempo até chegar ao consumidor final.
Effect of Prewetting Brines and Mixing on Ice-Melting Rate of Salt at Cold Temperatures: New Tracer Dilution Method
Authors: Koefod Scott
Published: May 2017

A novel test method has been developed to measure the ice-melting rate of deicers. The ice-melting rates of prewetted salt were determined by measuring the change in the concentration of chloride (Cl−) or magnesium or calcium cations (Mg2+ or Ca2+, respectively) in the ice melt as tracers. The method is substantially more precise than the SHRP H205.1 standard and has the further advantage of measuring ice-melting and salt dissolution rates simultaneously. Brines were preequilibrated with ice at −19.3°C (−2.7°F) and blended with solid salt to determine the effect of different prewetting brines on the ice-melting rate of the solid salt component only. The measured equilibrium ice-melting capacity of sodium chloride (NaCl) agreed well with the theoretical value calculated from the NaCl freezing point curve. Under a condition of no mixing, solid salt yielded 0.87% of its total available ice-melting capacity after 60 min when wetted with NaCl brine and 9.7% when wetted with calcium chloride (CaCl2) brine. Mixing raised the yield of ice melt to 27.1% and 50.5% after 60 min when wet with NaCl and CaCl2 brines, respectively. The CaCl2 brine was slightly more effective than the magnesium chloride (MgCl2) brine at enhancing the ice-melting rate of salt. The test method promises to be a useful tool for permitting a more precise optimization of prewetting brine composition, concentration, and brine-to-salt ratio at different temperatures. The method may also permit better determination of the cost-effectiveness of different prewetting strategies and provide deeper insights into the mechanism of chemical ice melting.
Editorial
Authors: Pernau Margrit
Published: May 2017

With this issue, the editorial team transition that has been going on for the
past year is complete. Sinai Rusinek has been at the head of Contributions since
2009. The first two years were spent finding a new home for the journal. Since
2011, we have been publishing with Berghahn Journals, and the Van Leer Jerusalem
Institute has assisted us with a generous grant, for which we are truly
grateful.
Introduction
Authors: Goudarouli Eirini
Published: May 2017

The special section “Knowledge Quests in the European Periphery” attempts
to explore the different ways in which conceptual history’s methodologies
could be applied to disciplines with which traditional conceptual historians
have not previously engaged, such as the history of science, political economy,
Enlightenment studies, postcolonial history, and transnational history. This
special section, when read as a whole, opens up a multidisciplinary space in
which center-periphery tensions are examined in the context of conceptual
transnational exchange. Coming from different geographical places and cultural
spaces within the European periphery, the three case studies draw their
methodological background from conceptual history and aim to reflect on
the center-periphery dichotomy by asking how historians from different historiographical
traditions could take advantage of the methods and theories
of conceptual history, as well as how conceptual history could take advantage
of the coming together of disciplines that traditionally do not communicate
with each other.
Identification of Successful Cognitive Aging in the Alzheimer’s Disease Neuroimaging Initiative Study
Authors: Lin Feng V. , Wang Xixi , Wu Rachel , Rebok George W. , Chapman Benjamin P. ,   the Alzheimer’s Disease Neuroimaging Initiative
Published: May 2017

The present prospective observational study aimed to identify the existence of successful cognitive agers among a group of well-defined cognitively healthy older adults ( n = 354, mean age = 75 years), and to examine baseline individual-level predictors and associated health outcomes over time. Episodic memory (EM) and executive function (EF) composite scores and multiple health outcomes were obtained annually over 5 years. Potential individual-level predictors that were related to Alzheimer’s disease pathology or genetic risk, neurodegeneration, and vascular risks were collected at baseline. Three latent classes with matched age and education were identified using growth mixture modeling: a group of participants who exhibited high, stable EM and EF (40.7% of the sample, “successful agers”); a group who had initial high cognitive performance that declined over time (21.2%, “declining agers”); and a group who had normal (EM) or poor (EF) but stable cognitive performance over time (38.1%, “low stable agers”). The group classification predicted significant differences in the incidence of global cognitive impairment, the development of at least one depressive symptom, and everyday functional impairment. Sex, apolipoprotein E allele 4, amyloid-β1-42, and t-tau significantly contributed to the difference in cognitive trajectories between the successful agers and the other two groups. Characterizing successful cognitive agers who are relatively resistant to both tau and amyloid pathology provides potential pathways for promoting successful cognitive aging and preventing cognitive decline.
Blood Pressure and Hispanic/Latino Cognitive Function: Hispanic Community Health Study/Study of Latinos Results
Authors: Tarraf Wassim , Rodríguez Carlos J. , Daviglus Martha L. , Lamar Melissa , Schneiderman Neil , Gallo Linda , Talavera Gregory A. , Kaplan Robert C. , Fornage Myriam , Conceicao Alan , González Hector M.
Published: May 2017

Background: Hispanics/Latinos are at increased risk for cardiovascular disease and cognitive decline and dementias. High blood pressure (BP) has been implicated in both stroke and dementias. Associations between BP and cognition among diverse Latinos are still unpublished.

Objective: We examined associations between cognition and four BP based measures among diverse Hispanics/Latinos. We hypothesized that higher BP, particularly systolic pressure, and increased arterial stiffness (i.e., pulse pressure), would be associated with lower cognitive function.

Methods: We used baseline (2008–2011) Hispanic Community Health Study/Study of Latinos (HCHS/SOL; n = 9,019; ages 45–74 years) data to examine cognition in relation to BP measures.

Results: In age, sex, and education adjusted models, systolic, pulse, and mean arterial pressure were consistently negatively associated with executive function, psychomotor speed and sustained attention, verbal episodic learning and memory, speech fluency, and mental status measures. These associations were attenuated but remained statistically significant in fully adjusted models.

Conclusion: Among middle-aged and older diverse Hispanics/Latinos, we found modest but consistent associations between indicators of arterial stiffness, and compromised blood flow and lower cognitive function. Clinical management and public health interventions to raise awareness and enhance BP management beginning in midlife could reduce disparities and improve population health by reducing cognitive decline burdens.
No Genetic Overlap Between Circulating Iron Levels and Alzheimer’s Disease
Authors: Lupton Michelle K. , Benyamin Beben , Proitsi Petroula , Nyholt Dale R. , Ferreira Manuel A. , Montgomery Grant W. , Heath Andrew C. , Madden Pamela A. , Medland Sarah E. , Gordon Scott D. ,   GERAD1 Consortium,   the Alzheimer’s Disease Neuroimaging Initiative, Lovestone Simon , Tsolaki Magda , Kloszewska Iwona , Soininen Hilkka , Mecocci Patrizia , Vellas Bruno , Powell John F. , Bush Ashley I. , Wright Margaret J. , Martin Nicholas G. , Whitfield John B.
Published: May 2017

Iron deposition in the brain is a prominent feature of Alzheimer’s disease (AD). Recently, peripheral iron measures have also been shown to be associated with AD status. However, it is not known whether these associations are causal: do elevated or depleted iron levels throughout life have an effect on AD risk? We evaluate the effects of peripheral iron on AD risk using a genetic profile score approach by testing whether variants affecting iron, transferrin, or ferritin levels selected from GWAS meta-analysis of approximately 24,000 individuals are also associated with AD risk in an independent case-control cohort (n∼10,000). Conversely, we test whether AD risk variants from a GWAS meta-analysis of approximately 54,000 account for any variance in iron measures (n∼9,000). We do not identify a genetic relationship, suggesting that peripheral iron is not causal in the initiation of AD pathology.
Motoric Cognitive Risk Syndrome: Association with Incident Dementia and Disability
Authors: Doi Takehiko , Shimada Hiroyuki , Makizako Hyuma , Tsutsumimoto Kota , Verghese Joe , Suzuki Takao
Published: May 2017

Background: It is important to examine the etiology of motoric cognitive risk syndrome (MCR) and its association with dementia and disability to obtain biological insights and to develop preventive strategies.

Objective: This study aimed to examine the association of MCR with incidence of dementia and disability in a Japanese community-dwelling sample of older adults.

Methods: Participants were 4,235 older adults (50% women, mean age: 72.0 years). MCR was diagnosed at baseline using established criteria in non-demented seniors with self-reported cognitive complaints and slow gait. Incident cases of dementia were identified from insurance data monthly. Disability was regarded as certification by long-term care insurance.

Results: At baseline, 265 participants (6.3%) met criteria for MCR. During follow-up (mean duration: 29 months), there were 138 incident cases of dementia (3.3%) and 207 incident cases of disability (4.9%). Cox-proportional hazards models, adjusted for demographical data, lifestyle, and medical conditions, showed that presence of MCR at baseline was a major risk factor for developing dementia (HR 2.49, 95% CI 1.52–4.10, p < 0.001). MCR also predicted risk for disability (HR 1.69, 95% CI 1.08–2.02, p < 0.001).

Conclusions: MCR is helpful in the short-term prediction of risk for dementia and disability in the elderly Japanese population. Identification of seniors with MCR is recommended for early detection and instituting preventive measures for reducing the risk of dementia and disability.
Postnatal Development of Spasticity Following Transgene Insertion in the Mouse βIV Spectrin Gene (SPTBN4)
Authors: Kichkin Eva , Visvanathan Archunan , Lovicu Frank J. , Shu Daisy Y. , Das Shannon J. , Reddel Stephen W. , McCann Emily P. , Zhang Katharine Y. , Williams Kelly L. , Blair Ian P. , Phillips William D.
Published: May 2017

Background: The L25 mouse line was generated by random genomic insertion of a lens-specific transgene. Inbreeding of L25 hemizygotes revealed an unanticipated spastic phenotype in the hind limbs.

Objective: The goals were to characterize the motor phenotype in the L25 mice and to map the transgene insert site within the mouse genome.

Methods: Six pairs of L25+/– mice were repeatedly mated. Beginning at weaning, all progeny were inspected for body weight and motor signs twice weekly until they displayed predefined ethical criteria for termination. The transgene insert site was determined by whole genome sequencing. Western blotting was used to compare the expression levels of beta-IV spectrin protein in the brain.

Results: Matings of hemizygous L25+/– × L25+/– mice yielded 20% (29/148) affected weanlings, identified by an abnormal retraction of the hind limbs when lifted by the tail, and a fine tremor. Affected mice were less mobile and grew more slowly than wild-type littermates. All affected mice required termination due to >15% loss of body weight (50% survival age 92 days). At the endpoint, mice showed varying degrees of spastic paresis or spastic paralysis localised to the hind limbs. Motor endplates remained fully innervated. Genome sequencing confirmed that the transgene was inserted in the locus of βIV spectrin of L25 mice. Western blotting indicated that this random insertion had greatly reduced the expression of βIV spectrin protein in the affected L25 mice.

Conclusions: The results confirm the importance of βIV spectrin for maintaining central motor pathway control of the hind limbs, and provide a developmental time course for the phenotype.
Correction: A No Reference Image Quality Assessment Metric Based on Visual Perception. Algorithms 2016, 9, 87
Authors: Fu Yan , Wang Shengchun
Published: May 2017

We would like to make the following change to our article [1]. [...]
Exploring Rooftop Rainwater Harvesting Potential for Food Production in Urban Areas
Authors: Lupia Flavio , Baiocchi Valerio , Lelo Keti , Pulighe Giuseppe
Published: May 2017

Homegrown fruits and vegetables are gaining popularity in many metropolitan areas with several facets connected to the wider urban agriculture phenomenon. At the same time, the relationship between urban food production and irrigation water is pivotal in terms of resource management. In this paper, we investigated water savings through the collection and use of harvestable rainwater from buildings’ rooftops to irrigate 2631 fruits and vegetables gardens in the urban area of Rome (Italy). The methodology makes use of existing geospatial data and data derived from satellite image classification to estimate food gardens’ irrigation requirements and harvestable rainwater from nearby buildings’ rooftops. The comparison of the annual harvestable rainwater with irrigation needs allowed for computing the proportion of water self-sufficient gardens as well as the amount of gardens whose water needs might be partially fulfilled with rainwater. Statistics were produced by land use type (horticulture, mixed crops, olive groves, orchards, and vineyards) and under the hypothesis that irrigation systems with low and high field application efficiency might be employed. We found that 19% and 33% of the gardens could be water self-sufficient for the low and high irrigation efficiency scenario, respectively. The remaining gardens, by using the available rainwater, could satisfy 22% (low efficiency) and 44% (high efficiency) of the water needs resulting in a reduction in the use of conventional water sources.
Microbial Carbon Substrate Utilization Differences among High- and Average-Yield Soybean Areas
Authors: Adams Taylor , Brye Kristofor , Savin Mary , Lee Jung Ae , Gbur Edward
Published: May 2017

Since soybean (Glycine max L. (Merr.)) yields greater than 6719 kg ha−1 have only recently and infrequently been achieved, little is known about the soil microbiological environment related to high-yield soybean production. Soil microbiological properties are often overlooked when assessing agronomic practices for optimal production. Therefore, a greater understanding is needed regarding how soil biological properties may differ between high- and average-yielding areas within fields. The objectives of this study were to (i) evaluate the effects of region on soil microbial carbon substrate utilization differences between high- (HY) and average-yield (AY) areas and (ii) assess the effect of yield area on selected microbiological property differences. Replicate soil samples were collected from the 0–10 cm depth from yield-contest-entered fields in close proximity that had both a HY and an AY area. Samples were collected immediately prior to or just after soybean harvest in 2014 and 2015 from each of seven geographic regions within Arkansas. Averaged across yield area, community-level carbon substrate utilization and Shannon’s and Simpson’s functional diversity and evenness were greater (p < 0.05) in Region 7 than all other regions. Averaged across regions, Shannon’s functional diversity and evenness were greater (p < 0.05) in HY than in AY areas. Principal component analysis demonstrated that a greater variety of carbon substrates were used in HY than AY areas. These results may help producers understand the soil microbiological environment in their own fields that contribute to or hinder achieving high-yielding soybeans; however, additional parameters may need to be assessed for a more comprehensive understanding of the soil environment that is associated with high-yielding soybean.
A National Census of Birth Weight in Purebred Dogs in Italy
Authors: Groppetti Debora , Pecile Alessandro , Palestrini Clara , Marelli Stefano , Boracchi Patrizia
Published: May 2017

Despite increasing professionalism in dog breeding, the physiological range of birth weight in this species remains unclear. Low birth weight can predispose to neonatal mortality and growth deficiencies in humans. To date, the influence of the morphotype on birth weight has never been studied in dogs. For this purpose, an Italian census of birth weight was collected from 3293 purebred pups based on maternal morphotype, size, body weight and breed, as well as on litter size and sex of pups. Multivariate analysis outcomes showed that birth weight (p < 0.001) and litter size (p < 0.05) increased with maternal size and body weight. Birth weight was also influenced by the maternal head and body shape, with brachycephalic and brachymorph dogs showing the heaviest and the lightest pups, respectively (p < 0.001). Birth weight decreased with litter size (p < 0.001), and male pups were heavier than females (p < 0.001). These results suggest that canine morphotype, not only maternal size and body weight, can affect birth weight and litter size with possible practical implications in neonatal assistance.
Is Root Catalase a Bifunctional Catalase-Peroxidase?
Authors: Chioti Vasileia , Zervoudakis George
Published: May 2017

Plant catalases exhibit spatial and temporal distribution of their activity. Moreover, except from the typical monofunctional catalase, a bifunctional catalase-peroxidase has been reported. The aim of this study was to investigate whether the leaf and root catalases from six different plant species (Lactuca sativa, Cichorium endivia, Apium graveolens, Petroselinum crispum, Lycopersicon esculentum, and Solanum melongena) correspond to the monofunctional or the bifunctional type based on their sensitivity to the inhibitor 3-amino-1,2,4-triazole (3-AT). The leaf catalases from all species seem to be monofunctional since they are very sensitive to 3-AT. On the other hand, the root enzymes from Lactuca sativa, Cichorium endivia, Lycopersicon esculentum, and Solanum melongena seem to be bifunctional catalase-peroxidases, considering that they are relatively insensitive to 3-AT, whereas the catalases from Apium graveolens and Petroselinum crispum display the same monofunctional characteristics as the leaves’ enzymes. The leaf catalase activity is usually higher (Lactuca sativa, Petroselinum crispum, and Solanum melongena) or similar (Cichorium endivia and Apium graveolens) to the root one, except for the enzyme from Lycopersicon esculentum, while in all plant species the leaf protein concentration is significantly higher than the root protein concentration. These results suggest that there are differences between leaf and root catalases—differences that may correspond to their physiological role.
Optimal Design of an Air-to-Air Heat Exchanger with Cross-Corrugated Triangular Ducts by Using a Particle Swarm Optimization Algorithm
Authors: Liang Caihang , Tong Xiaoman , Lei Tengyue , Li Zhenxing , Wu Guoshan
Published: May 2017

Air-to-air heat exchangers with cross-corrugated triangular ducts are widely used in various industrial fields to recover waste heat. The geometric parameters of the heat exchangers greatly affect the performance and total annual cost of these systems. In this study, the effectiveness-number of transfer units (ε-NTU) method was utilized to develop the thermal mathematical model, which was verified by comparing it with previous research. The configuration parameters of the heat exchanger were optimized in this study. The particle swarm optimization (PSO) algorithm was applied using both single and multi-objective algorithm. The colburn factor (j factor), friction factor (f factor), and comprehensive thermal hydraulic performance index (JF factor) were considered as objective functions to be optimized using a single objective and multi-objective algorithm. Then, the entropy generation rate and total annual cost were optimized by using a multi-objective PSO algorithm. In addition, to identify the influential geometric parameters, a global sensitivity analysis was performed. The sensitivity analysis showed that the apex angle θ, channel height H, and heat exchanger height Lh influenced the performance and annual total cost of these systems.
Rogue Wave Modes for the Coupled Nonlinear Schrödinger System with Three Components: A Computational Study
Authors: Chan Hiu Ning , Chow Kwok Wing
Published: May 2017

The system of “integrable” coupled nonlinear Schrödinger equations (Manakov system) with three components in the defocusing regime is considered. Rogue wave solutions exist for a restricted range of group velocity mismatch, and the existence condition correlates precisely with the onset of baseband modulation instability. This assertion is further elucidated numerically by evidence based on the generation of rogue waves by a single mode disturbance with a small frequency. This same computational approach can be adopted to study coupled nonlinear Schrödinger equations for the “non‐integrable” regime, where the coefficients of self‐phase modulation and cross‐phase modulation are different from each other. Starting with a wavy disturbance of a finite frequency corresponding to the large modulation instability growth rate, a breather can be generated. The breather can be symmetric or asymmetric depending on the magnitude of the growth rate. Under the presence of a third mode, rogue wave can exist under a larger group velocity mismatch between the components as compared to the two‐component system. Furthermore, the nonlinear coupling can enhance the maximum amplitude of the rogue wave modes and bright four‐petal configuration can be observed.
Detailed Opacity Calculations for Astrophysical Applications
Authors: Pain Jean-Christophe , Gilleron Franck , Comet Maxime
Published: May 2017

Nowadays, several opacity codes are able to provide data for stellar structure models, but the computed opacities may show significant differences. In this work, we present state-of-the-art precise spectral opacity calculations, illustrated by stellar applications. The essential role of laboratory experiments to check the quality of the computed data is underlined. We review some X-ray and XUV laser and Z-pinch photo-absorption measurements as well as X-ray emission spectroscopy experiments involving hot dense plasmas produced by ultra-high-intensity laser irradiation. The measured spectra are systematically compared with the fine-structure opacity code SCO-RCG. The focus is on iron, due to its crucial role in understanding asteroseismic observations of β Cephei-type and Slowly Pulsating B stars, as well as of the Sun. For instance, in β Cephei-type stars, the iron-group opacity peak excites acoustic modes through the “kappa-mechanism”. Particular attention is paid to the higher-than-predicted iron opacity measured at the Sandia Z-machine at solar interior conditions. We discuss some theoretical aspects such as density effects, photo-ionization, autoionization or the “filling-the-gap” effect of highly excited states.
Molecular Diagnostic for Prospecting Polyhydroxyalkanoate-Producing Bacteria
Authors: Montenegro Eduarda , Delabary Gabriela , Silva Marcus , Andreote Fernando , Lima André
Published: May 2017

The use of molecular diagnostic techniques for bioprospecting and microbial diversity study purposes has gained more attention thanks to their functionality, low cost and quick results. In this context, ten degenerate primers were designed for the amplification of polyhydroxyalkanoate synthase (phaC) gene, which is involved in the production of polyhydroxyalkanoate (PHA)—a biodegradable, renewable biopolymer. Primers were designed based on multiple alignments of phaC gene sequences from 218 species that have their genomes already analyzed and deposited at Biocyc databank. The combination of oligos phaCF3/phaCR1 allowed the amplification of the expected product (PHA synthases families types I and IV) from reference organisms used as positive control (PHA producer). The method was also tested in a multiplex system with two combinations of initiators, using 16 colonies of marine bacteria (pre-characterized for PHA production) as a DNA template. All amplicon positive organisms (n = 9) were also PHA producers, thus no false positives were observed. Amplified DNA was sequenced (n = 4), allowing for the confirmation of the phaC gene identity as well its diversity among marine bacteria. Primers were also tested for screening purposes using 37 colonies from six different environments. Almost 30% of the organisms presented the target amplicon. Thus, the proposed primers are an efficient tool for screening bacteria with potential for the production of PHA as well to study PHA genetic diversity.
Acute Regression in Young People with Down Syndrome
Authors: Mircher Clotilde , Cieuta-Walti Cécile , Marey Isabelle , Rebillat Anne-Sophie , Cretu Laura , Milenko Eliane , Conte Martine , Sturtz Franck , Rethore Marie-Odile , Ravel Aimé
Published: May 2017

Abstract: Adolescents and young adults with Down syndrome (DS) can present a rapid regression with loss of independence and daily skills. Causes of regression are unknown and treatment is most of the time symptomatic. We did a retrospective cohort study of regression cases: patients were born between 1959 and 2000, and were followed from 1984 to now. We found 30 DS patients aged 11 to 30 years old with history of regression. Regression occurred regardless of the cognitive level (severe, moderate, or mild intellectual disability (ID)). Patients presented psychiatric symptoms (catatonia, depression, delusions, stereotypies, etc.), partial or total loss of independence in activities of daily living (dressing, toilet, meals, and continence), language impairment (silence, whispered voice, etc.), and loss of academic skills. All patients experienced severe emotional stress prior to regression, which may be considered the trigger. Partial or total recovery was observed for about 50% of them. In our cohort, girls were more frequently affected than boys (64%). Neurobiological hypotheses are discussed as well as preventative and therapeutic approaches.
Adventure Behavior Seeking Scale
Authors: Próchniak Piotr
Published: May 2017

This article presents a new tool—the Adventure Behavior Seeking Scale (ABSS). The Adventure Behavior Seeking Scale was developed to assess individuals’ highly stimulating behaviors in natural environments. An exploratory factor analysis was conducted with 466 participants and resulted in one factor. The internal consistency was 0.80. A confirmatory factor analysis was performed using another sample of 406 participants, and results verified the one-factor structure. The findings indicate that people with a lot of experience in outdoor adventure have a higher score on the ABSS scale than control groups without such experience. The results also suggest that the 8-item ABSS scores were highly related to sensation seeking. The author discusses findings in regard to the ABSS as an instrument to measure outdoor adventure. However, further studies need to be carried out in other sample groups to further validate the scale.
Improving Outcomes in Patients with CRC: The Role of Patient Reported Outcomes—An ESDO Report
Authors: Van Cutsem Eric , De Gramont Aimery , Henning Geoffrey , Rougier Philippe , Bonnetain Franck , Seufferlein Thomas
Published: May 2017

Colorectal cancer is one of the most commonly diagnosed cancers worldwide and nearly half of patients will develop metastatic disease at some point during the course of their disease. The goal of anticancer therapy in this context is to extend survival, while trying to maximise the patient’s health-related quality of life. To this end, we need to understand how to incorporate patient-reported outcomes into clinical trials and routine practice to accurately assess if treatment strategies are providing clinical benefit for the patient. This review reflects the proceedings of a 2016 European Society of Digestive Oncology workshop, where the authors discussed the use of patient-reported outcomes to measure health-related quality of life when evaluating treatment during the management of colorectal cancer. A summary of the challenges associated with implementing patient-reported outcomes in clinical trials is provided, as well as a review of the current clinical evidence surrounding patient-reported outcomes in metastatic colorectal cancer.
Elevational Trends in Usnic Acid Concentration of Lichen Parmelia flexilis in Relation to Temperature and Precipitation
Authors: Neupane Bishnu , Malla Komal , Gautam Anil , Chaudhary Dinesh , Paudel Sanjita , Timsina Sangita , Jamarkattel Nirmala
Published: May 2017

Usnic acid contents in acetone extracts of 31 samples of lichen Parmelia flexilis collected from different altitudes were identified using thin layer chromatography (TLC) and determined by high performance liquid chromatography (HPLC). The usnic acid content varied in between highest 5.13% to lowest 1.66% in oven dried (80 °C) lichen samples. The species collected from lower altitudes all show high levels of usnic acid. The negative relationship between usnic acid and altitude was obtained. Statistically, it is revealed that there is a significant difference between average percentages of usnic acid in lichen samples with varying altitudes (p < 0.05). Beside these, the precipitation averages of the regions where the species have been collected were linked with the content of usnic acid. It is clear that lichens from the regions receiving the highest precipitation produced lower amounts of usnic acid. The results suggest that the production of secondary metabolite in lichens is altered due to the climatic variables like temperature and precipitation at different altitude gradients.
Improved Cell Search for mmWave Cellular Networks Using Deterministic Scanning Algorithm with Directional Array Antenna
Authors: Vo Dai Toan , Kilic Ozlem , Liu Hang
Published: May 2017

Millimeter Wave (mmWave) communication is considered as an enabling technology for the next generation of cellular networks because it offers much larger bandwidth and higher data rate than the current lower-frequency cellular systems to satisfy the exponential growth of mobile data demand. High gain directional antennas are needed to overcome high propagation losses in mmWave bands. However, the reliance on highly directional antennas will result in a more complicated initial cell search procedure since both base station and mobile device have to look for each other over a large space to establish the link. This paper focuses on analyzing the performance of the directional cell search procedure where the base stations periodically transmit signals in a set of optimal directional patterns to scan the coverage area. The mobile terminals detect the signals from the base station using the Generalized Likelihood Ratio Test (GLRT). The results show that with an appropriate scanning scheme, the use of directional antennas can outperform their omnidirectional counterparts in terms of signal detection performance as well as total time required.
Substrate Chemistry and Rainfall Regime Regulate Elemental Composition of Tree Leaves in Karst Forests
Authors: Medina Ernesto , Cuevas Elvira , Lugo Ariel
Published: May 2017

Forests on calcareous substrates constitute a large fraction of the vegetation in Puerto Rico. Plant growth on these substrates may be affected by nutrient deficiencies, mainly P and Fe, resulting from high pH and formation of insoluble compounds of these elements. The occurrence of these forests in humid and dry areas provides an opportunity to compare nutrient relations, water use efficiency, and N dynamics, using biogeochemical parameters. We selected sites under humid climate in the north, and dry climate in the southwest of Puerto Rico. Adult, healthy leaves of species with high importance values were collected at each site and analyzed for their elemental composition and the natural abundance of C and N isotopes. Calcium was the dominant cation in leaf tissues, explaining over 70% of the ash content variation, and Al and Ca concentration were positively correlated, excepting only two Al-accumulating species. Karst vegetation consistently showed high N/P ratios comparable to forests on P-poor soils. Dry karst sites had significantly higher δ13C and δ15N ratios. We conclude that forests on karst are mainly limited by P availability, and that mechanisms of nutrient uptake in the rhizosphere lead to linear correlations in the uptake of Ca and Al. Isotope ratios indicate higher water use efficiency, and predominant denitrification in dry karst forest sites.
Living and Dead Aboveground Biomass in Mediterranean Forests: Evidence of Old-Growth Traits in a Quercus pubescens Willd. s.l. Stand
Authors: Badalamenti Emilio , La Mantia Tommaso , La Mantia Giovanni , Cairone Antonino , La Mela Veca Donato
Published: May 2017

For a long time, human impact has deeply simplified most of the forest ecosystems of the Mediterranean Basin. Here, forests have seldom had the chance to naturally develop a complex and multilayered structure, to host large and old trees and rich biological communities, approaching old-growth conditions. Also for this reason, limited information is currently available about Mediterranean old-growth forests, particularly with regard to deadwood. The main aim of this work is to help fill this critical knowledge gap. In Sicily (Italy), we identified a Quercus pubescens forest that seemed to show some typical old-growth features. Total living volume (360 m3 ha−1) and basal area (34 m2 ha−1) were, respectively, about 6 and 3 times higher than the averages recorded in the regional forest inventory for this forest type. Deadwood was particularly abundant, exceeding the threshold of 30 m3 ha−1, mainly represented by lying dead elements. Dead to live wood ratio reached 9%, a value close to the threshold of 10% considered for Mediterranean old-growth forests. As the investigated forest showed some typical old-growth traits, it deserves to be fully protected and could be a permanent monitoring area for studying deadwood and stand dynamics in mature Mediterranean stands.
New Knowledge in Analytical, Technological, and Biological Aspects of the Maillard Reaction
Authors: Delgado-Andrade Cristina
Published: May 2017

The Maillard reaction (MR) is the non-enzymatic browning reaction that can occur both in foods and in living beings.[...]
Security Investment, Hacking, and Information Sharing between Firms and between Hackers
Authors: Hausken Kjell
Published: May 2017

A four period game between two firms and two hackers is analyzed. The firms first defend and the hackers thereafter attack and share information. Each hacker seeks financial gain, beneficial information exchange, and reputation gain. The two hackers’ attacks and the firms’ defenses are inverse U-shaped in each other. A hacker shifts from attack to information sharing when attack is costly or the firm’s defense is cheap. The two hackers share information, but a second more disadvantaged hacker receives less information, and mixed motives may exist between information sharing and own reputation gain. The second hacker’s attack is deterred by the first hacker’s reputation gain. Increasing information sharing effectiveness causes firms to substitute from defense to information sharing, which also increases in the firms’ unit defense cost, decreases in each firm’s unit cost of own information leakage, and increases in the unit benefit of joint leakage. Increasing interdependence between firms causes more information sharing between hackers caused by larger aggregate attacks, which firms should be conscious about. We consider three corner solutions. First and second, the firms deter disadvantaged hackers. When the second hacker is deterred, the first hacker does not share information. Third, the first hacker shares a maximum amount of information when certain conditions are met. Policy and managerial implications are provided for how firms should defend against hackers with various characteristics.
Work-Related Musculoskeletal Disorders and Risk Factors among Chinese Medical Staff of Obstetrics and Gynecology
Authors: Wang Jingjing , Cui Ya , He Lihua , Xu Xiangrong , Yuan Zhiwei , Jin Xianning , Li Zhimin
Published: May 2017

Medical staff in the department of obstetrics and gynecology are a group of professionals reportedly at high risk of work-related musculoskeletal disorders (WMSD), however, little is known about the current status of this problem in China. The aim of this study was to investigate prevalence and risk factors of work-related musculoskeletal disorders among this population in China. A self-developed questionnaire was distributed to 1017 obstetrics and gynecology practitioners to collect information on musculoskeletal symptoms and relevant factors. Prevalence and severity of work-related musculoskeletal disorders in different parts of the body were calculated and the relationship between personal and ergonomic factors and work-related musculoskeletal disorders was analyzed using Chi-square test and unconditional logistic regression models. The results indicated a high prevalence of 85.5% among the subjects, with the shoulder (n = 575, 62.0%), neck (n = 560, 60.3%) and lower back (n = 504, 54.3%) being the three most affected regions. Individual, postural, work-environmental as well as psychosocial factors were recognized to be associated with WMSDs in different body parts. Therefore, attention must be given to the problem of musculoskeletal disorders among Chinese obstetrics and gynecology staff. It is recommended to develop good life habits, improve work environment, adjust work organization as well as train on proper postures in their daily operation.
Socioeconomic, Geospatial, and Geopolitical Disparities in Access to Health Care in the US 2011–2015
Authors: Towne Jr. Samuel D.
Published: May 2017

Individuals forgoing needed medical care due to barriers associated with cost are at risk of missing needed care that may be necessary for the prevention or maintenance of a chronic condition among other things. Thus, continued monitoring of factors associated with forgone medical care, especially among vulnerable populations, is critical. National survey data (2011–2015) for non-institutionalized adults residing in the USA were utilized to assess forgone medical care, defined as not seeking medical care when the individual thought it was necessary because of cost in the past 12 months. Logistic regression was used to predict forgone medical care vs. sought medical care. Racial/ethnic minority working-age adults, those with lower incomes, those with lower educations, those residing in the South, and those residing in states that failed to participate in Medicaid Expansion in 2014 were more likely (p < 0.01) to forgo medical care due to cost in the past year. Policy makers seeking to reduce barriers to forgone medical care can use this information to tailor their efforts (e.g., mechanisms targeted to bridge gaps in access to care) to those most at-risk and to consider state-level policy decisions that may impact access to care.
Extraction of Terraces on the Loess Plateau from High-Resolution DEMs and Imagery Utilizing Object-Based Image Analysis
Authors: Zhao Hanqing , Fang Xuan , Ding Hu , Josef Strobl , Xiong Liyang , Na Jiaming , Tang Guoan
Published: May 2017

Abstract: Terraces are typical artificial landforms on the Loess Plateau, with ecological functions in water and soil conservation, agricultural production, and biodiversity. Recording the spatial distribution of terraces is the basis of monitoring their extent and understanding their ecological effects. The current terrace extraction method mainly relies on high-resolution imagery, but its accuracy is limited due to vegetation coverage distorting the features of terraces in imagery. High-resolution topographic data reflecting the morphology of true terrace surfaces are needed. Terraces extraction on the Loess Plateau is challenging because of the complex terrain and diverse vegetation after the implementation of “vegetation recovery”. This study presents an automatic method of extracting terraces based on 1 m resolution digital elevation models (DEMs) and 0.3 m resolution Worldview-3 imagery as auxiliary information used for object-based image analysis (OBIA). A multi-resolution segmentation method was used where slope, positive and negative terrain index (PN), accumulative curvature slope (AC), and slope of slope (SOS) were determined as input layers for image segmentation by correlation analysis and Sheffield entropy method. The main classification features based on DEMs were chosen from the terrain features derived from terrain factors and texture features by gray-level co-occurrence matrix (GLCM) analysis; subsequently, these features were determined by the importance analysis on classification and regression tree (CART) analysis. Extraction rules based on DEMs were generated from the classification features with a total classification accuracy of 89.96%. The red band and near-infrared band of images were used to exclude construction land, which is easily confused with small-size terraces. As a result, the total classification accuracy was increased to 94%. The proposed method ensures comprehensive consideration of terrain, texture, shape, and spectrum characteristics, demonstrating huge potential in hilly-gully loess region with similarly complex terrain and diverse vegetation covers.
C1q/TNF-Related Protein-9 Ameliorates Ox-LDL-Induced Endothelial Dysfunction via PGC-1α/AMPK-Mediated Antioxidant Enzyme Induction
Authors: Sun Haijian , Zhu Xuexue , Zhou Yuetao , Cai Weiwei , Qiu Liying
Published: May 2017

Oxidized low-density lipoprotein (ox-LDL) accumulation is one of the critical determinants in endothelial dysfunction in many cardiovascular diseases such as atherosclerosis. C1q/TNF-related protein 9 (CTRP9) is identified to be an adipocytokine with cardioprotective properties. However, the potential roles of CTRP9 in endothelial function remain largely elusive. In the present study, the effects of CTRP9 on the proliferation, apoptosis, migration, angiogenesis, nitric oxide (NO) production and oxidative stress in human umbilical vein endothelial cells (HUVECs) exposed to ox-LDL were investigated. We observed that treatment with ox-LDL inhibited the proliferation, migration, angiogenesis and the generation of NO, while stimulated the apoptosis and reactive oxygen species (ROS) production in HUVECs. Incubation of HUVECs with CTRP9 rescued ox-LDL-induced endothelial injury. CTRP9 treatment reversed ox-LDL-evoked decreases in antioxidant enzymes including heme oxygenase-1 (HO-1), nicotinamide adenine dinucleotide phosphate (NAD(P)H) dehydrogenase quinone 1, and glutamate-cysteine ligase (GCL), as well as endothelial nitric oxide synthase (eNOS). Furthermore, CTRP9 induced activation of peroxisome proliferator-activated receptor γ co-activator 1α (PGC1-α) and phosphorylation of adenosine monophosphate-activated protein kinase (AMPK). Of interest, AMPK inhibition or PGC1-α silencing abolished CTRP9-mediated antioxidant enzymes levels, eNOS expressions, and endothelial protective effects. Collectively, we provided the first evidence that CTRP9 attenuated ox-LDL-induced endothelial injury by antioxidant enzyme inductions dependent on PGC-1α/AMPK activation.
Use of the Ketogenic Diet to Treat Intractable Epilepsy in Mitochondrial Disorders
Authors: Paleologou Eleni , Ismayilova Naila , Kinali Maria
Published: May 2017

Mitochondrial disorders are a clinically heterogeneous group of disorders that are caused by defects in the respiratory chain, the metabolic pathway of the adenosine tri-phosphate (ATP) production system. Epilepsy is a common and important feature of these disorders and its management can be challenging. Epileptic seizures in the context of mitochondrial disease are usually treated with conventional anti-epileptic medication, apart from valproic acid. However, in accordance with the treatment of intractable epilepsy where there are limited treatment options, the ketogenic diet (KD) has been considered as an alternative therapy. The use of the KD and its more palatable formulations has shown promising results. It is especially indicated and effective in the treatment of mitochondrial disorders due to complex I deficiency. Further research into the mechanism of action and the neuroprotective properties of the KD will allow more targeted therapeutic strategies and thus optimize the treatment of both epilepsy in the context of mitochondrial disorders but also in other neurodegenerative disorders.