bluuebunny/crossref_metadata_2025 huggingface dataset:
Hydroclimate Variations across North-Central China during the Past 530 Years and Their Relationships with Atmospheric Oscillations
Authors: Kang Shuyuan , Liu Jingjing , Wang Jianglin
Published: March 2023

Detailed study of historical drought events in North-Central China (NCC) is important to understand current hydroclimate variability in the background of global warming. Here, we combined 12 published tree-ring chronologies and 12 dryness/wetness indices (DWI) to reconstruct dry and wet climate variability across NCC. These 24 proxy records showed similarly significant responses to warm season (May–June–July–August–September, MJJAS) moisture signals. A new 530-year-long reconstruction of self-calibrating Palmer Drought Severity Index (scPDSI) values for the warm season in NCC was determined using a nested principal component regression (PCR) approach. The new reconstruction shows significant correlations with the instrumental MJJAS scPDSI data across NCC during the period AD 1901–2012. The reconstructed MJJAS scPDSI revealed seven severe dry/wet events from AD 1470 to 2012. The periods AD 1701–1727 and AD 1985–2011 represent the longest dry periods, and the drought during the 1920s is identified as the most severe one over the past 530 years. Our reconstruction shows significant interannual spectral peaks at the frequency domain of 2–7 years, together with relatively weaker decadal frequencies of 16, 24, and 78 years. The results of superposed epoch analysis (SEA) show that extreme North Atlantic Oscillation (NAO) years may modulate drought variability in NCC.
Fermentation Dynamics of Naturally Fermented Palm Beverages of West Bengal and Jharkhand in India
Authors: Das Souvik , Tamang Jyoti Prakash
Published: March 2023

The term ‘toddy’ represents a group of different varieties of mild-alcoholic palm beverages of coastal and inland India, produced from the fresh saps of various palm trees through uncontrolled natural fermentation. In this study, we analysed the successional changes of microbial abundances and various physico-chemical parameters during natural fermentation (0 h to 48 h) of taal toddy, prepared from Palmyra palm, and khejur toddy, prepared from date palm of West Bengal and Jharkhand in India. Microorganisms from different successional levels were isolated and grouped using repetitive element sequence-based PCR (rep-PCR) technique and identified by the sequencing of 16S rRNA gene and D1-D2 region of 26S rRNA gene for bacteria and yeasts, respectively. Enterococcus faecalis, Lactiplantibacillus plantarum, Lacticaseibacillus paracasei and yeast Saccharomyces cerevisiae were identified during natural fermentation of toddy. During the natural fermentation, the average pH and total sugar content in the samples of both taal and khejur toddy decreased, whereas a gradual rise was observed in the contents of acidity, total alcohol, total ester and total protein. Bio-active potential (presence of phenolics and flavonoids) of toddy was also analysed (0 h to 48 h), where contents of total phenolics, flavonoids and resulting anti-oxidant activity were found higher in the end-product than the fresh palm sap, indicating toddy as a functional low-alcoholic drink. Lastly, it can be concluded that the inter-variable dynamics and microbial interrelation, which in turn depend on a number of local factors, regulate the overall fermentation dynamics and determine the product quality.
Two-Stage Process for Energy Valorization of Cheese Whey through Bio-Electrochemical Hydrogen Production Coupled with Microbial Fuel Cell
Authors: Zonfa Tatiana , Kamperidis Theofilos , Falzarano Marica , Lyberatos Gerasimos , Polettini Alessandra , Pomi Raffaella , Rossi Andreina , Tremouli Asimina
Published: March 2023

The present work investigates a two-stage process scheme for cheese whey valorization through energy recovery in different forms by means of bio-electrochemical systems. The first stage consisted of an integrated bio-electrochemical process for H2 and electricity production. This combined dark fermentation with an electrochemical system with the aim of overcoming the typical thermodynamic/biochemical limitations of fermentation and enhancing H2 recovery. The second treatment stage involved a single-chamber microbial fuel cell, featuring an innovative configuration consisting of four air cathodes with fly ash as the oxygen reduction catalyst. The bio-electrochemical process performed in the first stage achieved promising results, displaying a three-times higher H2 production yield compared to conventional dark fermentation. In addition, the experiments using the MFC in the second stage were found to successfully exploit the effluent from the first stage, with COD removal yields of 86% ± 8% and energy recovery with a maximum current output of 1.6 mA and a maximum power density of 1.2 W/m3.
Production and Analytical Aspects of Natural Pigments to Enhance Alternative Meat Product Color
Authors: Bakhsh Allah , Cho Changjun , Baritugo Kei Anne , Kim Bosung , Ullah Qamar , Rahman Attaur , Park Sungkwon
Published: March 2023

Color is a major feature that strongly influences the consumer’s perception, selection, and acceptance of various foods. An improved understanding regarding bio-safety protocols, health welfare, and the nutritional importance of food colorants has shifted the attention of the scientific community toward natural pigments to replace their toxic synthetic counterparts. However, owing to safety and toxicity concerns, incorporating natural colorants directly from viable sources into plant-based meat (PBM) has many limitations. Nonetheless, over time, safe and cheap extraction techniques have been developed to extract the purified form of coloring agents from raw materials to be incorporated into PBM products. Subsequently, extracted anthocyanin has displayed compounds like Delphinidin-3-mono glucoside (D3G) at 3.1 min and Petunidin-3-mono glucoside (P3G) at 5.1 277, 515, and 546 nm at chromatographic lambda. Fe-pheophytin was successfully generated from chlorophyll through the ion exchange method. Likewise, the optical density (OD) of synthesized leghemoglobin (LegH) indicated that pBHA bacteria grow more rigorously containing ampicillin with a dilution factor of 10 after 1 h of inoculation. The potential LegH sequence was identified at 2500 bp through gel electrophoresis. The color coordinates and absorbance level of natural pigments showed significant differences (p < 0.05) with the control. The development of coloring agents originating from natural sources for PBM can be considered advantageous compared to animal myoglobin in terms of health and functionality. Therefore, the purpose of this study was to produce natural coloring agents for PBM by extracting and developing chlorophyll from spinach, extracting anthocyanins from black beans, and inserting recombinant plasmids into microorganisms to produce LegH.
Foliar Selenate and Zinc Oxide Separately Applied to Two Pea Varieties: Effects on Growth Parameters and Accumulation of Minerals and Macronutrients in Seeds under Field Conditions
Authors: Malka Maksymilian , Laing Gijs Du , Hegedűsová Alžbeta , Bohn Torsten
Published: March 2023

Though selenium (Se) and zinc (Zn) constitute essential nutrients for human health, their deficiencies affect up to 15% and 17% of the global population, respectively. Agronomic biofortification of staple crops with Se/Zn may alleviate these challenges. Pea (Pisum sativum L.) is a nutritious legume crop that has great potential for Se/Zn biofortification. Herein, two varieties of pea (Ambassador, Premium) were biofortified via foliar application of sodium selenate (0/50/100 g of Se/ha) or zinc oxide (0/375/750 g of Zn/ha) during the flowering stage under field conditions. While no significant differences were found in Se accumulation between seed varieties upon Se treatments, selenate enhanced the accumulation of Se in the two seed varieties in a dose dependent manner. Selenium concentration was most elevated in seeds of Ambassador exposed to 100 g of Se/ha (3.93 mg/kg DW compared to the control (0.08 mg/kg DW), p < 0.001). 375 g of Zn/ha (35.7 mg/kg DW) and 750 g of Zn/ha (35.5 mg/kg DW) significantly and similarly enhanced Zn concentrations compared to the control (31.3 mg/kg DW) in Premium seeds, p < 0.001. Zinc oxide also improved accumulations of Fe, Cu, Mn, and Mg in Premium seeds. Se/Zn treatments did not significantly affect growth parameters and accumulations of soluble solids and protein in seeds. Positive and significant (p < 0.01) correlations were observed between Zn and Fe, Cu, Mn and Mg levels in Premium seeds, among others. Consuming 33 g/day of pea biofortified with Se at 50 g/ha and 266 g/day of pea biofortified with 375 g of Zn/ha could provide 100% of the RDA (55 μg) for Se and RDA (9.5 mg) for Zn in adults, respectively. These results are relevant for enhancing Se/Zn status in peas by foliar biofortification.
Improvement of Oxidative Stability of Fish Oil-in-Water Emulsions through Partitioning of Sesamol at the Interface
Authors: Gao Zhihui , Ji Zhongyan , Wang Leixi , Deng Qianchun , Quek Siew Young , Liu Liang , Dong Xuyan
Published: March 2023

The susceptibility of polyunsaturated fatty acids to oxidation severely limits their application in functional emulsified foods. In this study, the effect of sesamol concentration on the physicochemical properties of WPI-stabilized fish oil emulsions was investigated, focusing on the relationship between sesamol–WPI interactions and interfacial behavior. The results relating to particle size, zeta-potential, microstructure, and appearance showed that 0.09% (w/v) sesamol promoted the formation of small oil droplets and inhibited oil droplet aggregation. Furthermore, the addition of sesamol significantly reduced the formation of hydrogen peroxide, generation of secondary reaction products during storage, and degree of protein oxidation in the emulsions. Molecular docking and isothermal titration calorimetry showed that the interaction between sesamol and β-LG was mainly mediated by hydrogen bonds and hydrophobic interactions. Our results show that sesamol binds to interfacial proteins mainly through hydrogen bonding, and increasing the interfacial sesamol content reduces the interfacial tension and improves the physical and oxidative stability of the emulsion.
Stress at Slaughter: A Key Factor in the Determination of Meat Quality?
Authors: Terlouw Claudia , Gagaoua Mohammed
Published: March 2023

Meat consumption has played an important role in human evolution [...]
Brewer’s Spent Grain, Coffee Grounds, Burdock, and Willow–Four Examples of Biowaste and Biomass Valorization through Advanced Green Extraction Technologies
Authors: Errico Massimiliano , Coelho Jose A. P. , Stateva Roumiana P. , Christensen Knud V. , Bahij Rime , Tronci Stefania
Published: March 2023

This paper explores the transformation of biowastes from food industry and agriculture into high-value products through four examples. The objective is to provide insight into the principles of green transition and a circular economy. The first two case studies focus on the waste generated from the production of widely consumed food items, such as beer and coffee, while the other two examine the potential of underutilized plants, such as burdock and willow, as sources of valuable compounds. Phenolic compounds are the main target in the case of brewer’s spent grain, with p-coumaric acid and ferulic acid being the most common. Lipids are a possible target in the case of spent coffee grounds with palmitic (C16:0) and linoleic (C18:2) acid being the major fatty acids among those recovered. In the case of burdock, different targets are reported based on which part of the plant is used. Extracts rich in linoleic and oleic acids are expected from the seeds, while the roots extracts are rich in sugars, phenolic acids such as chlorogenic, caffeic, o-coumaric, syringic, cinnamic, gentisitic, etc. acids, and, interestingly, the high-value compound epicatechin gallate. Willow is well known for being rich in salicin, but picein, (+)-catechin, triandrin, glucose, and fructose are also obtained from the extracts. The study thoroughly analyzes different extraction methods, with a particular emphasis on cutting-edge green technologies. The goal is to promote the sustainable utilization of biowaste and support the green transition to a more environmentally conscious economy.
Mode of Antifungal Action of Daito-Gettou (Alpinia zerumbet var. exelsa) Essential Oil against Aspergillus brasiliensis
Authors: Okazaki Kiyo , Sumitani Hidenobu , Takahashi Katsutada , Isegawa Yuji
Published: March 2023

Plant-derived essential oils (EOs) are used in medicines, disinfectants, and aromatherapy products. Information on the antifungal activity of EO of Alpinia zerumbet var. exelsa (known as Daito-gettou) found in Kitadaito Island, Okinawa, is limited. Therefore, we aimed to evaluate the antifungal activity of EOs obtained via steam distillation of leaves of Daito-gettou, which is a hybrid of A. zerumbet and A. uraiensis. Daito-gettou EO showed antifungal activity (minimum inhibitory concentration = 0.4%) against Aspergillus brasiliensis NBRC 9455, which was comparable to that of A. zerumbet found in the Okinawa main island. Gas chromatography/mass spectrometry revealed that the main components of Daito-gettou EOs are γ-terpinene, terpinen-4-ol, 1,8-cineole, 3-carene, and p-cymene. Terpinen-4-ol content (MIC = 0.075%) was 17.24%, suggesting that the antifungal activity of Daito-gettou EO was mainly attributable to this component. Daito-gettou EO and terpinen-4-ol inhibited mycelial growth. Moreover, calorimetric observations of fungal growth in the presence of Daito-gettou EO showed a characteristic pattern with no change in the initial growth rate and only a delay in growth. As this pattern is similar to that of amphotericin B, it implies that the action mode of Daito-gettou EO and terpinen-4-ol may be fungicidal. Further studies on the molecular mechanisms of action are needed for validation.
Composition, Microbiota, Mechanisms, and Anti-Obesity Properties of Rice Bran
Authors: Sivamaruthi Bhagavathi Sundaram , Alagarsamy Karthikeyan , Thangaleela Subramanian , Bharathi Muruganantham , Kesika Periyanaina , Chaiyasut Chaiyavat
Published: March 2023

Rice is a major cereal crop and a staple food for nearly 50% of people worldwide. Rice bran (RB) is a nutrient-rich by-product of rice processing. RB is rich in carbohydrates, fibers, proteins, lipids, minerals, and several trace elements (phosphorus, calcium, magnesium, potassium, and manganese). The extraction process and storage have influenced RB extracts and RB oil’s quality. The RB composition has also varied on the rice cultivars. The color of RB indicates the richness of the bioactive compounds, especially anthocyanins. γ-oryzanol, tocopherols, tocotrienols, and unsaturated fatty acids are major components of RB oil. It has been established that RB supplementation could improve the host’s health status. Several preclinical and clinical studies have reported that RB has antioxidant, anticancer, anti-inflammatory, anticolitis, and antidiabetic properties. The beneficial biological properties of RB are partially attributed to its ability to alter the host microbiome and help to maintain and restore eubiosis. Non-communicable diseases (NCDs), including heart disease, diabetes, cancer, and lung disease, account for 74% of deaths worldwide. Obesity is a global health problem and is a major reason for the development of NCDs. The medical procedures for managing obesity are expensive and long-term health supplements are required to maintain a healthy weight. Thus, cost-effective natural adjuvant therapeutic strategy is crucial to treat and manage obesity. Several studies have revealed that RB could be a complementary pharmacological candidate to treat obesity. A comprehensive document with basic information and recent scientific results on the anti-obesity activity of RB and RB compounds is obligatory. Thus, the current manuscript was prepared to summarize the composition of RB and the influence of RB on the host microbiome, possible mechanisms, and preclinical and clinical studies on the anti-obesity properties of RB. This study suggested that the consumption of RB oil and dietary RB extracts might assist in managing obesity-associated health consequences. Further, extended clinical studies in several ethnic groups are required to develop dietary RB-based functional and nutritional supplements, which could serve as an adjuvant therapeutic strategy to treat obesity.
Antioxidant Activity and Inhibition of Liver Cancer Cells’ Growth of Extracts from 14 Marine Macroalgae Species of the Mediterranean Sea
Authors: Goutzourelas Nikolaos , Kevrekidis Dimitrios Phaedon , Barda Sofia , Malea Paraskevi , Trachana Varvara , Savvidi Stavroula , Kevrekidou Alkistis , Assimopoulou Andreana N. , Goutas Andreas , Liu Ming , Lin Xiukun , Kollatos Nikolaos , Amoutzias Grigorios D. , Stagos Dimitrios
Published: March 2023

Macroalgae exhibit beneficial bioactivities for human health. Thus, the aim of the present study was to examine the antioxidant and anticancer potential of 14 macroalgae species’ extracts, namely, Gigartina pistillata, Gigartina teedei, Gracilaria gracilis, Gracilaria sp., Gracilaria bursa pastoris, Colpomenia sinuosa, Cystoseira amentacea, Cystoseira barbata, Cystoseira compressa, Sargassum vulgare, Padina pavonica, Codium fragile, Ulva intestinalis, and Ulva rigida, from the Aegean Sea, Greece. The antioxidant activity was assessed using DPPH, ABTS•+, •OH, and O2•− radicals’ scavenging assays, reducing power (RP), and protection from ROO•-induced DNA plasmid damage assays. Moreover, macroalgae extracts’ total polyphenol contents (TPCs) were assessed. Extracts’ inhibition against liver HepG2 cancer cell growth was assessed using the XTT assay. The results showed that G. teedei extract’s IC50 was the lowest in DPPH (0.31 ± 0.006 mg/mL), ABTS•+ (0.02 ± 0.001 mg/mL), •OH (0.10 ± 0.007 mg/mL), O2•− (0.05 ± 0.003 mg/mL), and DNA plasmid breakage (0.038 ± 0.002 mg/mL) and exhibited the highest RP (RP0.5AU 0.24 ± 0.019 mg/mL) and TPC (12.53 ± 0.88 mg GAE/g dw). There was also a significant correlation between antioxidant activity and TPC. P. pavonica (IC50 0.93 ± 0.006 mg/mL) exhibited the highest inhibition against HepG2 cell growth. Conclusively, some of the tested extracts exhibited significant chemopreventive properties, and so they may be used for food products.
Factors Influencing Consumers’ Willingness-to-Try Seafood Byproducts
Authors: Murillo Silvia , Ardoin Ryan , Prinyawiwatkul Witoon
Published: March 2023

With increasing global demand for seafood, seafood byproducts (SB) utilization can contribute to a more sustainable food supply chain through waste-to-value food product development. However, consumer perceptions of SB (e.g., fish skin and bones) are underexplored. Therefore, this study aims to evaluate some factors influencing consumers’ willingness-to-try seafood byproducts. An online survey was conducted in the USA regarding intervention of SB informational cues with N = 904 adult seafood consumers internationally. The proportion of consumers willing to try SB increased significantly (McNemar’s test, α = 0.05) from 47% to 68% after SB safety and health claims had been presented in the questionnaire. Gender, race, SB knowledge, and previous SB consumption were significant predictors of trial intent (based on logistic regression), as were emotional baseline scores during the COVID-19 pandemic. Males were more open to SB consumption than females, and racial identity was associated with differential responsiveness to SB information. Higher levels of “bored” and “unsafe” feelings, and lower levels of “free” were associated with increased SB trial intent. Potential SB consumers identified fish products (82% willingness-to-try); seasoning mix, sauces, and dressing (71% willingness-to-try); and soup and gravy products (62% willingness-to-try) as most appropriate for SB incorporation. Predominant reasons for SB avoidance were concerns about sensory quality, safety, and nutrition. These consumer-driven data could guide SB product development concepts to encourage trial and overcome aversions through new consumption experience.
Combined Effects of Acrylamide and Ochratoxin A on the Intestinal Barrier in Caco-2 Cells
Authors: Su Dan , Lu Jiawen , Nie Chunchao , Guo Ziyan , Li Chang , Yu Qiang , Xie Jianhua , Chen Yi
Published: March 2023

Acrylamide (AA) and ochratoxin A (OTA) are contaminants that co-exist in the same foods, and may create a serious threat to human health. However, the combined effects of AA and OTA on intestinal epithelial cells remain unclear. The purpose of this research was to investigate the effects of AA and OTA individually and collectively on Caco-2 cells. The results showed that AA and OTA significantly inhibited Caco-2 cell viability in a concentration- and time-dependent manner, decreased transepithelial electrical resistance (TEER) values, and increased the lucifer yellow (LY) permeabilization, lactate dehydrogenase (LDH) release and reactive oxygen species (ROS) levels. In addition, the levels of IL-1β, IL-6, and TNF-α increased, while the levels of IL-10 decreased after AA and OTA treatment. Western blot analysis revealed that AA and OTA damaged the intestinal barrier by reducing the expression of the tight junction (TJ) protein. The collective effects of AA and OTA exhibited enhanced toxicity compared to either single compound and, for most of the intestinal barrier function indicators, AA and OTA combined exposure tended to produce synergistic toxicity to Caco-2 cells. Overall, this research suggests the possibility of toxic reactions arising from the interaction of toxic substances present in foodstuffs with those produced during processing.
Evaluation of Parity Effect on Characteristics and Minerals in Buffalo (Bubalus Bubalis) Colostrum and Mature Milk
Authors: An Zhigao , Luo Gan , Gao Shanshan , Zhang Xinxin , Chen Chao , Yao Zhiqiu , Zhao Junwei , Lv Haimiao , Niu Kaifeng , Nie Pei , Yang Liguo
Published: March 2023

Colostrum is a vital performance for buffaloes and potentially functional foods in the future. Therefore, this study aimed to evaluate the difference between the parity of buffalo colostrum and mature milk. Twenty pregnant buffaloes (primiparous = 10; multiparous = 10) were assigned to the same diet prepartum and milking routine postpartum. Calves were separated from the dams immediately after birth and colostrum was harvested within 2 h, whilst mature milk was harvested at 7 days postpartum. The colostrum was analyzed for immunoglobulin G and milk composition as the mature milk. The results showed that there was a higher level of protein, solid not fat, and milk urea nitrogen (p < 0.05), with a tendency for higher total solids (p = 0.08) in primiparous buffaloes’ colostrum compared with multiparous. No parity effect was observed in colostrum immunoglobulin G, fat, lactose, and yields of colostrum and composition (p > 0.05). There was no difference in mature milk composition and yield by parity affected (p > 0.05). Compared with mature milk composition, colostrum had a higher content protein, total solids, solid not fat, and milk urea nitrogen (p < 0.05); however, fat and lactose were lower than that of mature milk (p < 0.05). For minerals, multiparous buffaloes’ colostrum had a higher concentration of Fe (p = 0.05), while the mature milk had higher concentrations of K and P compared with primiparous. Buffalo colostrum had higher concentrations of Na, Mg, Co, Fe, and K with a lower concentration of Ca relative to mature milk (p < 0.05). It was observed that parity affected colostrum characteristics rather than mature milk and caused subtle variations in minerals in colostrum and mature milk of buffaloes. As lactation proceeded, both milk composition and minerals in the milk changed drastically.
Fractal-Based Approaches to Pore Structure Investigation and Water Saturation Prediction from NMR Measurements: A Case Study of the Gas-Bearing Tight Sandstone Reservoir in Nanpu Sag
Authors: Xie Weibiao , Yin Qiuli , Zeng Jingbo , Wang Guiwen , Feng Cheng , Zhang Pan
Published: March 2023

Pore space of tight sandstone samples exhibits fractal characteristics. Nuclear magnetic resonance is an effective method for pore size characterization. This paper focuses on fractal characteristics of pore size from nuclear magnetic resonance (NMR) of tight sandstone samples. The relationship between the fractal dimension from NMR with pore structure and water saturation is parameterized by analyzing experimental data. Based on it, a pore structure characterization and classification method for water-saturated tight sandstone and a water saturation prediction method in a gas-bearing sandstone reservoir have been proposed. To verify the models, the fractal dimension from NMR of 19 tight sandstone samples selected from the gas-bearing tight sandstone reservoir of Shahejie Formation in Nanpu Sag and that of 16 of them under different water saturation states are analyzed. The application result of new methods in the gas-bearing tight sandstone reservoir of Shahejie Formation in Nanpu Sag shows consistency with experimental data. This paper has facilitated the development of the NMR application by providing a non-electrical logging idea in reservoir quality evaluation and water saturation prediction. It provides a valuable scientific resource for reservoir engineering and petrophysics of unconventional reservoir types, such as tight sandstone, low porosity, and low permeability sandstone, shale, and carbonate rock reservoirs.
Monitoring the Size and Flux Density of Sgr A* during the Active State in 2019 with East Asian VLBI Network
Authors: Cheng Xiaopeng , Cho Ilje , Kawashima Tomohisa , Kino Motoki , Zhao Guang-Yao , Algaba Juan-Carlos , Kofuji Yutaro , Lee Sang-Sung , Lee Jee-Won , Cheong Whee Yeon , Jiang Wu , Oh Junghwan
Published: March 2023

In this work, we studied the Galactic Center supermassive black hole (SMBH), Sagittarius A* (Sgr A*), with the KVN and VERA Array (KaVA)/East Asian VLBI Network (EAVN) monitoring observations. Especially in 13 May 2019, Sgr A* experienced an unprecedented bright near infra-red (NIR) flare; so, we find a possible counterpart at 43 GHz (7 mm). As a result, a large temporal variation of the flux density at the level ∼15.4%, with the highest flux density of 2.04 Jy, is found on 11 May 2019. Interestingly, the intrinsic sizes are also variable, and the area and major-axis size show marginal correlation with flux density with ≳2 σ. Thus, we interpret that the emission region at 43 GHz follows the larger-when-brighter relation in 2019. The possible origins are discussed with an emergence of a weak jet/outflow component and the position angle change of the rotation axis of the accretion disk in time.
Study on the Interaction of Plasma-Polymerized Hydrogel Coatings with Aqueous Solutions of Different pH
Authors: Levien Monique , Nasri Zahra , Weltmann Klaus-Dieter , Fricke Katja
Published: March 2023

Amphiphilic hydrogels from mixtures of 2-hydroxyethyl methacrylate and 2-(diethylamino)ethyl methacrylate p(HEMA-co-DEAEMA) with specific pH sensitivity and hydrophilic/hydrophobic structures were designed and polymerized via plasma polymerization. The behavior of plasma-polymerized (pp) hydrogels containing different ratios of pH-sensitive DEAEMA segments was investigated concerning possible applications in bioanalytics. In this regard, the morphological changes, permeability, and stability of the hydrogels immersed in solutions of different pHs were studied. The physico-chemical properties of the pp hydrogel coatings were analyzed using X-ray photoelectron spectroscopy, surface free energy measurements, and atomic force microscopy. Wettability measurements showed an increased hydrophilicity of the pp hydrogels when stored in acidic buffers and a slightly hydrophobic behavior after immersion in alkaline solutions, indicating a pH-dependent behavior. Furthermore, the pp (p(HEMA-co-DEAEMA) (ppHD) hydrogels were deposited on gold electrodes and studied electrochemically to investigate the pH sensitivity of the hydrogels. The hydrogel coatings with a higher ratio of DEAEMA segments showed excellent pH responsiveness at the studied pHs (pH 4, 7, and 10), demonstrating the importance of the DEAEMA ratio in the functionality of pp hydrogel films. Due to their stability and pH-responsive properties, pp (p(HEMA-co-DEAEMA) hydrogels are conceivable candidates for functional and immobilization layers for biosensors.
Preclinical Potential of Probiotic-Loaded Novel Gelatin–Oil Vaginal Suppositories: Efficacy, Stability, and Safety Studies
Authors: Bassi Anchal , Sharma Garima , Deol Parneet Kaur , Madempudi Ratna Sudha , Kaur Indu Pal
Published: March 2023

The current study describes a suppository base composed of aqueous gelatin solution emulsifying oil globules with probiotic cells dispersed within. The favorable mechanical properties of gelatin to provide a solid gelled structure, and the tendency of its proteins to unravel into long strings that interlace when cooled, lead to a three-dimensional structure that can trap a lot of liquid, which was exploited herein to result in a promising suppository form. The latter maintained incorporated probiotic spores of Bacillus coagulans Unique IS-2 in a viable but non-germinating form, preventing spoilage during storage and imparting protection against the growth of any other contaminating organism (self-preserved formulation). The gelatin–oil–probiotic suppository showed uniformity in weight and probiotic content (23 ± 2.481 × 108 cfu) with favorable swelling (double) followed by erosion and complete dissolution within 6 h of administration, leading to the release of probiotic (within 45 min) from the matrix into simulated vaginal fluid. Microscopic images indicated presence of probiotics and oil globules enmeshed in the gelatin network. High viability (24.3 ± 0.46 × 108), germination upon application and a self-preserving nature were attributed to the optimum water activity (0.593 aw) of the developed composition. The retention of suppositories, germination of probiotics and their in vivo efficacy and safety in vulvovaginal candidiasis murine model are also reported.
Research Advances on Hydrogel-Based Materials for Tissue Regeneration and Remineralization in Tooth
Authors: Zhang Zhijun , Bi Fei , Guo Weihua
Published: March 2023

Tissue regeneration and remineralization in teeth is a long-term and complex biological process, including the regeneration of pulp and periodontal tissue, and re-mineralization of dentin, cementum and enamel. Suitable materials are needed to provide cell scaffolds, drug carriers or mineralization in this environment. These materials need to regulate the unique odontogenesis process. Hydrogel-based materials are considered good scaffolds for pulp and periodontal tissue repair in the field of tissue engineering due to their inherent biocompatibility and biodegradability, slow release of drugs, simulation of extracellular matrix, and the ability to provide a mineralized template. The excellent properties of hydrogels make them particularly attractive in the research of tissue regeneration and remineralization in teeth. This paper introduces the latest progress of hydrogel-based materials in pulp and periodontal tissue regeneration and hard tissue mineralization and puts forward prospects for their future application. Overall, this review reveals the application of hydrogel-based materials in tissue regeneration and remineralization in teeth.
Synthesis of NVCL-NIPAM Hydrogels Using PEGDMA as a Chemical Crosslinker for Controlled Swelling Behaviours in Potential Shapeshifting Applications
Authors: Tie Billy Shu Hieng , Halligan Elaine , Zhuo Shuo , Keane Gavin , Geever Luke
Published: March 2023

Stimuli-responsive hydrogels have recently gained interest within shapeshifting applications due to their capabilities to expand in water and their altering swelling properties when triggered by stimuli, such as pH and heat. While conventional hydrogels lose their mechanical strength during swelling, most shapeshifting applications require materials to have mechanical strength within a satisfactory range to perform specified tasks. Thus, stronger hydrogels are needed for shapeshifting applications. Poly (N-isopropylacrylamide) (PNIPAm) and poly (N-vinyl caprolactam) (PNVCL) are the most popular thermosensitive hydrogels studied. Their close-to-physiological lower critical solution temperature (LCST) makes them superior candidates in biomedicine. In this study, copolymers made of NVCL and NIPAm and chemically crosslinked using poly (ethylene glycol) dimethacrylate (PEGDMA) were fabricated. Successful polymerisation was proven via Fourier transform infrared spectroscopy (FTIR). The effects of incorporating comonomer and crosslinker on the LCST were found minimal using cloud-point measurements, ultraviolet (UV) spectroscopy, and differential scanning calorimetry (DSC). Formulations that completed three cycles of thermo-reversing pulsatile swelling are demonstrated. Lastly, rheological analysis validated the mechanical strength of PNVCL, which was improved due to the incorporation of NIPAm and PEGDMA. This study showcases potential smart thermosensitive NVCL-based copolymers that can be applied in the biomedical shapeshifting area.
Research Progress of Polysaccharide-Based Natural Polymer Hydrogels in Water Purification
Authors: Zhang Wenxu , Xu Yan , Mu Xuyang , Li Sijie , Liu Xiaoming , Lei Ziqiang
Published: March 2023

The pollution and scarcity of freshwater resources are global problems that have a significant influence on human life. It is very important to remove harmful substances in the water to realize the recycling of water resources. Hydrogels have recently attracted attention due to their special three-dimensional network structure, large surface area, and pores, which show great potential for the removal of pollutants in water. In their preparation, natural polymers are one of the preferred materials because of their wide availability, low cost, and easy thermal degradation. However, when it is directly used for adsorption, its performance is unsatisfactory, so it usually needs to be modified in the preparation process. This paper reviews the modification and adsorption properties of polysaccharide-based natural polymer hydrogels, such as cellulose, chitosan, starch, and sodium alginate, and discusses the effects of their types and structures on performance and recent technological advances.
Transcriptional Profiling of Rat Prefrontal Cortex after Acute Inescapable Footshock Stress
Authors: Martini Paolo , Mingardi Jessica , Carini Giulia , Mattevi Stefania , Ndoj Elona , La Via Luca , Magri Chiara , Gennarelli Massimo , Russo Isabella , Popoli Maurizio , Musazzi Laura , Barbon Alessandro
Published: March 2023

Stress is a primary risk factor for psychiatric disorders such as Major Depressive Disorder (MDD) and Post Traumatic Stress Disorder (PTSD). The response to stress involves the regulation of transcriptional programs, which is supposed to play a role in coping with stress. To evaluate transcriptional processes implemented after exposure to unavoidable traumatic stress, we applied microarray expression analysis to the PFC of rats exposed to acute footshock (FS) stress that were sacrificed immediately after the 40 min session or 2 h or 24 h after. While no substantial changes were observed at the single gene level immediately after the stress session, gene set enrichment analysis showed alterations in neuronal pathways associated with glia development, glia–neuron networking, and synaptic function. Furthermore, we found alterations in the expression of gene sets regulated by specific transcription factors that could represent master regulators of the acute stress response. Of note, these pathways and transcriptional programs are activated during the early stress response (immediately after FS) and are already turned off after 2 h—while at 24 h, the transcriptional profile is largely unaffected. Overall, our analysis provided a transcriptional landscape of the early changes triggered by acute unavoidable FS stress in the PFC of rats, suggesting that the transcriptional wave is fast and mild, but probably enough to activate a cellular response to acute stress.
Current Status and Prospects of the Single-Cell Sequencing Technologies for Revealing the Pathogenesis of Pregnancy-Associated Disorders
Authors: Naydenov Dmitry , Vashukova Elena , Barbitoff Yury , Nasykhova Yulia , Glotov Andrey
Published: March 2023

Single-cell RNA sequencing (scRNA-seq) is a method that focuses on the analysis of gene expression profile in individual cells. This method has been successfully applied to answer the challenging questions of the pathogenesis of multifactorial diseases and open up new possibilities in the prognosis and prevention of reproductive diseases. In this article, we have reviewed the application of scRNA-seq to the analysis of the various cell types and their gene expression changes in normal pregnancy and pregnancy complications. The main principle, advantages, and limitations of single-cell technologies and data analysis methods are described. We discuss the possibilities of using the scRNA-seq method for solving the fundamental and applied tasks related to various pregnancy-associated disorders. Finally, we provide an overview of the scRNA-seq findings for the common pregnancy-associated conditions, such as hyperglycemia in pregnancy, recurrent pregnancy loss, preterm labor, polycystic ovary syndrome, and pre-eclampsia.
The Expression Patterns of Exogenous Plant miRNAs in Chickens
Authors: Li Hao , Zhang Pu , Li Diyan , Chen Binlong , Li Jing , Wang Tao
Published: March 2023

(1) Background: MicroRNAs (miRNAs) are involved in a variety of biological processes, such as cell proliferation, cell differentiation, and organ development. Recent studies have shown that plant miRNAs may enter the diet and play physiological and/or pathophysiological roles in human health and disease; however, little is known about plant miRNAs in chickens. (2) Methods: Here, we analyzed miRNA sequencing data, with the use of five Chinese native chicken breeds and six different tissues (heart, liver, spleen, lung, kidney, and leg muscle), and used Illumina sequencing to detect the expression of plant miRNAs in the pectoralis muscles at fourteen developmental stages of Tibetan chickens. (3) Results: The results showed that plant miRNAs are detectable in multiple tissues and organs in different chicken breeds. Surprisingly, we found that plant miRNAs, such as tae-miR2018, were detectable in free-range Tibetan chicken embryos at different stages. The results of gavage feeding experiments also showed that synthetic tae-miR2018 was detectable in caged Tibetan chickens after ingestion. The analysis of tae-miR2018 showed that its target genes were related to skeletal muscle organ development, regulation of mesodermal cell fate specification, growth factor activity, negative regulation of the cell cycle, and regulation of growth, indicating that exogenous miRNA may regulate the development of chicken embryos. Further cell cultures and exogenous miRNA uptake assay experiments showed that synthetic wheat miR2018 can be absorbed by chicken myoblasts. (4) Conclusions: Our study found that chickens can absorb and deposit plant miRNAs in various tissues and organs. The plant miRNAs detected in embryos may be involved in the development of chicken embryos.
Development of Improved DNA Collection and Extraction Methods for Handled Documents
Authors: Morgan Ashley G. , Prinz Mechthild
Published: March 2023

Handwritten documents may contain probative DNA, but most crime laboratories do not process this evidence. DNA recovery should not impair other evidence processing such as latent prints or indented writing. In this study, single fingermarks on paper were sampled with flocked swabs, cutting, and dry vacuuming. In addition, two extraction methods were compared for the sample type. DNA yields were low across all methods; however, this work confirms the ability to recover DNA from paper and the usefulness of the vacuum sampling method combined with the Chelex-Tween method. Stability of touch DNA deposits were compared over an 11-month period to better understand degradation that may occur over time. No significant difference in DNA recovery was observed, suggesting DNA deposits on paper are stable over an 11-month span.
Genetic Risk Scores and Missing Heritability in Ovarian Cancer
Authors: Fatapour Yasaman , Brody James P.
Published: March 2023

Ovarian cancers are curable by surgical resection when discovered early. Unfortunately, most ovarian cancers are diagnosed in the later stages. One strategy to identify early ovarian tumors is to screen women who have the highest risk. This opinion article summarizes the accuracy of different methods used to assess the risk of developing ovarian cancer, including family history, BRCA genetic tests, and polygenic risk scores. The accuracy of these is compared to the maximum theoretical accuracy, revealing a substantial gap. We suggest that this gap, or missing heritability, could be caused by epistatic interactions between genes. An alternative approach to computing genetic risk scores, using chromosomal-scale length variation should incorporate epistatic interactions. Future research in this area should focus on this and other alternative methods of characterizing genomes.
Comparison of Deterministic and Probabilistic Variational Data Assimilation Methods Using Snow and Streamflow Data Coupled in HBV Model for Upper Euphrates Basin
Authors: Uysal Gökçen , Alvarado-Montero Rodolfo , Şensoy Aynur , Şorman Ali Arda
Published: March 2023

The operation of upstream reservoirs in mountainous regions fed by snowmelt is highly challenging. This is partly due to scarce information given harsh topographic conditions and a lack of monitoring stations. In this sense, snow observations from remote sensing provide additional and relevant information about the current conditions of the basin. This information can be used to improve the model states of a forecast using data assimilation techniques, therefore enhancing the operation of reservoirs. Typical data assimilation techniques can effectively reduce the uncertainty of forecast initialization by merging simulations and observations. However, they do not take into account model, structural, or parametric uncertainty. The uncertainty intrinsic to the model simulations introduces complexity to the forecast and restricts the daily work of operators. The novel Multi-Parametric Variational Data Assimilation (MP-VarDA) uses different parameter sets to create a pool of models that quantify the uncertainty arising from model parametrization. This study focuses on the sensitivity of the parametric reduction techniques of MP-VarDA coupled in the HBV hydrological model to create model pools and the impact of the number of parameter sets on the performance of streamflow and Snow Cover Area (SCA) forecasts. The model pool is created using Monte Carlo simulation, combined with an Aggregated Distance (AD) Method, to create different model pool instances. The tests are conducted in the Karasu Basin, located at the uppermost part of the Euphrates River in Türkiye, where snowmelt is a significant portion of the yearly runoff. The analyses were conducted for different thresholds based on the observation exceedance probabilities. According to the results in comparison with deterministic VarDA, probabilistic MP-VarDA improves the m-CRPS gains of the streamflow forecasts from 57% to 67% and BSS forecast skill gains from 52% to 68% when streamflow and SCA are assimilated. This improvement rapidly increases for the first additional model parameter sets but reaches a maximum benefit after 5 parameter sets in the model pool. The improvement is notable for both methods in SCA forecasts, but the best m-CRPS gain is obtained for VarDA (31%), while the best forecast skill is detected in MP-VarDA (12%).
Year in Review: Gout Clinical Research
Authors: Urquiaga Mariana , Gaffo Angelo L.
Published: March 2023

Gout is a prevalent and burdensome condition despite the advances in our knowledge of its underlying mechanisms, prevention, and treatment. There is still work to be done to elucidate relevant questions that could lead to better patient care. This conference report summarizes eight impactful publications which inform and improve clinical care in gout from October 2021 to October 2022. The articles we present here address innovative management approaches, the use of serum urate as a surrogate marker, the occurrence of complications such as cardiovascular events and lower extremity amputation, the evaluation of mortality in patients with chronic kidney disease and gout, the effect of intensive serum urate control on radiographic outcomes, and the impact of COVID-19 infection in patients with gout. The conclusions reached by these publications are noteworthy. Some of them are potentially practice-changing, and all provide exciting follow-up questions.
Exploring Determinants of HIV/AIDS Self-Testing Uptake in South Africa Using Generalised Linear Poisson and Geographically Weighted Poisson Regression
Authors: Fundisi Emmanuel , Dlamini Simangele , Mokhele Tholang , Weir-Smith Gina , Motolwana Enathi
Published: March 2023

Increased HIV/AIDS testing is of paramount importance in controlling the HIV/AIDS pandemic and subsequently saving lives. Despite progress in HIV/AIDS testing programmes, most people are still reluctant to test and thus are still unaware of their status. Understanding the factors associated with uptake levels of HIV/AIDS self-testing requires knowledge of people’s perceptions and attitudes, thus informing evidence-based decision making. Using the South African National HIV Prevalence, HIV Incidence, Behaviour and Communication Survey of 2017 (SABSSM V), this study assessed the efficacy of Generalised Linear Poisson Regression (GLPR) and Geographically Weighted Poisson Regression (GWPR) in modelling the spatial dependence and non-stationary relationships of HIV/AIDS self-testing uptake and covariates. The models were calibrated at the district level across South Africa. Results showed a slightly better performance of GWPR (pseudo R2 = 0.91 and AICc = 390) compared to GLPR (pseudo R2 = 0.88 and AICc = 2552). Estimates of local intercepts derived from GWPR exhibited differences in HIV/AIDS self-testing uptake. Overall, the output of this study displays interesting findings on the levels of spatial heterogeneity of factors associated with HIV/AIDS self-testing uptake across South Africa, which calls for district-specific policies to increase awareness of the need for HIV/AIDS self-testing.
Ambient Noise in Candidate Rooms for User-Operated Audiometry
Authors: Sidiras Christos , Nielsen Jacob , Sørensen Chris Bang , Schmidt Jesper Hvass , Pedersen René Gyldenlund , Pedersen Ellen Raben
Published: March 2023

Hearing loss is a widespread problem while treatment is not always accessible, mainly because of the limited availability of hearing care professionals and clinics. In this work, part of the User-Operated Audiometry project, we investigate the acoustic environment of inexpensive non-sound-treated rooms that could be used for unsupervised audiometric testing. Measurements of 10 min of ambient noise were taken from 20 non-sound-treated rooms in libraries and private and public clinics, nine of which were measured twice. Ambient noise was compared against two traditional audiometric sound-treated rooms and Maximum Permissible Ambient noise levels by ISO 8231-1, while factoring for the attenuation by the DD450 circumaural headphones provided. In most non-sound-treated rooms, MPAs were violated only by transient sounds, while the floor-noise level was below MPAs. Non-sound-treated rooms’ ambient noise levels presented with much larger fluctuations compared to sound-treated rooms. Almost all violations occurred at low to mid-low frequencies. Our results suggest that large-scale implementation of user-operated audiometry outside traditional audiometric rooms is possible, at least under some realizable conditions. Circumaural headphones’ attenuation is probably a necessary condition for all cases. Depending on the room, an online system making decisions based on ambient noise might also be included in combination with active attenuation.