bluuebunny/crossref_metadata_2025 huggingface dataset:
Integrative multi-omic analyses identify candidate targets for celiac disease involving tissue-specific regulation
Authors: Sun Jiazheng , Zeng Yulan
Published: November 2024

Introduction:
Celiac disease (CeD) is an autoimmune condition characterized by a reversible inflammatory reaction in the mucous membrane of the small intestine. Nevertheless, there is a limited availability of efficient control approaches. Prior research has demonstrated that pharmacological targets supported by genetic evidence can greatly enhance the efficacy of drug development. Hence, the study aims to integrate transcriptomic and proteomic information to identify candidate targets for CeD.
Methods
The study employed proteome-wide Mendelian randomization (MR) analysis of circulating plasma proteins to investigate their causal association with CeD. The candidate targets for CeD were further assessed employing colocalization analysis, transcriptome-wide summary-data-based Mendelian randomization (SMR) analysis, multimarker analysis of genomic annotation (MAGMA) gene-based analysis, and bulk RNAseq-based differential expression analysis. For the proteins that were identified, extended Phenome-wide association studies (PheWAS) were conducted to assess their side-effect profiles, while the DGIdb database provided information on the approved or investigated drugs for candidate targets.
Results
Systematic MR analysis identified 22 candidate targets for CeD. Among the proteins analyzed, BTN2A1 passed all subsequent verification analyses. Additionally, three proteins, including CatH, IL-18R1, and PTPRC, passed the majority of the subsequent verification analyses. The other 18 proteins were also candidate targets (Trehalase, CD226, SH2B3, ICOSLG, ULK3, Park7, ALDH2, RABEP1, TNFRSF9, COL11A2, GNPDA1, IL-1RL1, B3galt6, TNFSF11, CCL21, BTN3A3, OLFM2 and Colipase).
Conclusions
The study employed a combination of human transcriptomic and proteomic information, employing several analytical methods. As a result, 22 proteins, divided into four tiers, were identified as prospective therapeutic targets for CeD.
Ecosystem Service Delivery by Urban Agriculture as a Nature-Based Solution (NBS): Carbon Sequestration
Authors: Gharibi Shiva , Shayesteh Kamran , Rastkhadiv Arman
Published: November 2024

To prevent the worst impacts of climate change, Nature-Based Solutions (NBSs), by maintaining and creating Urban Green Infrastructures (UGIs), can be used. Urban agriculture, as a UGI, is widely accepted as an NBS by providing a wide range of ecosystem services (ES), including Food production, wildlife Habitat, a sense of place and Educational opportunities, Nitrogen fixation, Flood reduction, Improving water quality, Local climate regulation, and Carbon storage and sequestration. This study aimed to investigate the potential of carbon storage and sequestration ES by urban agriculture (croplands) in the Hamadan urban area. First, the UGI map was prepared, and agricultural lands were extracted. Then, a stratified random sampling was used (120 sampling points). Soil sampling (plot 1×1 m), at a depth of 0–30 cm, was conducted, dried (at 105 ° C/48 hours), and analyzed for total carbon content by dry combustion method (550°C/2 hours). Grass and litter samples were harvested (plot 1×1 m), oven-dried (at 65 ° C/48 hours), and heated (at 115°C/24 hours). The results showed that the average carbon sequestration potential by soil, grass cover, and litter was 104.88, 4.96, and 0.27 t/ha, respectively. Potato (181 t/ha) and corn (58.8 t/ha) cultivation had the highest and lowest soil carbon sequestration values, respectively. Also, Flax grass cover has the highest carbon sequestration potential (14.33 t/ha), and tomato plants as a grass cover have the lowest potential (0.3 t/ha). The highest potential of carbon sequestration from the point of view of total soil and grass cover is for potato, flax, garlic, vegetable, abandoned, pea, fallow, wheat, tomato, cucumber, rapeseed, and corn, respectively. Finally, it can be concluded that urban agriculture can be an NBS for providing carbon sequestration ES.
Unifying Subicular Function: A Predictive Map Approach
Authors: Bennett Lauren , de Cothi William , Muessig Laurenz , Rodrigues Fabio Ribeiro , Cacucci Francesca , Wills Thomas , Sun Yanjun , Giocomo Lisa , Lever Colin , Poulter Steven , Barry Caswell
Published: November 2024

The successor representation has emerged as a powerful model for understanding mammalian navigation and memory; explaining the spatial coding properties of hippocampal place cells and entorhinal grid cells. However, the diverse spatial responses of subicular neurons, the primary output of the hippocampus, have eluded a unified account. Here, we demonstrate that incorporating rodent behavioural biases into the successor representation successfully reproduces the heterogeneous activity patterns of subicular neurons. This framework accounts for the emergence of boundary and corner cells; neuronal types absent in upstream hippocampal regions. We provide evidence that subicular firing patterns are more accurately described by the successor representation than a purely spatial or boundary vector cell model of subiculum. Our work offers a unifying theory of subicular function that positions the subiculum, more than other hippocampal regions, as a predictive map of the environment.
The visual representation of misinformation in the news
Authors: Al-Rawi Ahmed
Published: November 2024

This exploratory descriptive study examines the visual representation of misinformation in the news, partly relying on the automated labels associated with online and broadcast news such as OCR, face sentiments, labels, and logos detection. The findings of the study show that online and TV news organizations show varied amounts of attention in using misinformation-related terms to visually discuss events and issues, for CNN comes ahead in the US followed by MSNBC and Fox News. In terms of online news, The Daily Mail in the UK is the most active in visually covering misinformation. Many news organizations around the world are using the English language visual mark “Fake news” and related ones to fact check or discredit other sources of information. The study identifies other patterns of visual news coverage including sentiments associated with some political leaders and political polarization around issues related to misinformation such as COVID-19.
Epidemiology of TB screening among adults aged 18-64 years in South Africa in 2020
Authors: Tshuma Ndumiso , Malahlela Mokgadi , Mfundisi Mabalane , Simelane Xoliswa , Mbele-Khama Sheila , Mabusa Nickey , Raisibe Tshepang , Moyo Clinton , Ngbede Elakpa Daniel , Dlamini Sinothando , Ndhlovu Tshepo M , Ximiya Phumlani , Chadyiwa Martha , Mashau Ntsieni
Published: November 2024

Background
Tuberculosis (TB) continues to be a public health issue of concern in South Africa. The study aims to investigate the epidemiology of TB screening among people aged 18-64 years in South Africa.
Methods
This is a cross-sectional study with a study population of 132 adults in 6 provinces in South Africa. Data was collected through an online questionnaire. Outcome variable was self-reported TB screening within the past year. Descriptive statistics, multivariate logistic regression analysis were performed to determine prevalence and factors associated with TB screening, p-value <0.05 considered statistically significant.
Results
A third 37(38%) participants reported to have been screened for TB. Factors such as gender (AOR 1.54; p=0.490), participants in the age group 26-45 (AOR 7.80; p=0.010), Ever been tested for TB (AOR 23.17; p=0.000), and TB testing yearly (AOR 21.69; p=0.000) were positively associated with TB screening. Participants that had information on TB (AOR 0.20, p=0.031), and those who seek healthcare services (AOR 0.28, p=0.035) were significantly less likely to report TB screening.
Conclusion
Only a quarter of adults aged 18-64 in South Africa had been screened for TB, with significant associations observed for age, gender, previous TB screening, and recent TB testing. The structural barriers and the need for comprehensive, person-centered screening strategies to meet the WHO's 90% target are needed. The study recommends understanding the low utilization of health services, capacity building for healthcare providers, increasing awareness of TB symptoms, and conducting research on comprehensive TB screening approaches.
Mapping Current Services for Frequent Users of the Emergency Department in England
Authors: Saraiva Sonia , Lee William , Welsh Kate , Feltz-Cornelis Christina Van der , Pilkington Gerlinde , Ariss Steven , Burton Christopher , Mason Suzanne , Guthrie Elspeth
Published: November 2024

Background Frequent attendance at emergency departments (EDs) is a growing concern in the United Kingdom (UK), with about 2.5% of visitors accounting for approximately 10% of all ED attendances, leading to significant healthcare costs. Frequent users often face complex health issues, including high rates of multimorbidity. Despite various interventions aimed at reducing these visits, evidence from randomized controlled trials remains limited. The aim of this study was to better characterise current services in England for frequent users of the emergency department.Methods A regular national survey of liaison psychiatry services attached to all 171 acute hospitals in England was used to identify local frequent user services. The survey included questions about the existence and details of frequent user services at each hospital. Following data collection, the team categorized responses and selected 20 representative services for in-depth study, interviewing clinicians using a framework designed to describe healthcare organizations, focusing on capacity, organizational structure, financial mechanisms, patient characteristics, and care processes. Results: The survey had a 100% response. 157 acute hospitals (92%) were served by some form of service for frequent users. 81 hospitals (48%) reported frequent user services with dedicated staff time, 76 hospitals (44%) operated case management meetings but without designated staff, 14 hospitals reported no services for frequent users. Interviews conducted with 20 sites highlighted great variability amongst services on nearly all parameters including staffing levels, entry criteria, caseload, types of intervention and funding. Community-led services typically employed more staff and offered holistic, non-clinical social interventions compared to hospital-based services, which were more focused on case management meetings and psychological support/interventions.Conclusions The study revealed significant heterogeneity in services for frequent users of emergency departments. There is a need for clarity on intervention thresholds, types of interventions, and effective methods for evaluating outcomes to improve the consistency and effectiveness of these services to guide decision-making.
Markets For Children: International Adoptions, IVF, and U.S. Foster Care
Authors: Murugesan Anand , Innes Robert
Published: November 2024

The likelihood that a child in U.S. foster care is adopted depends upon alternatives available to parents. We analyzed a child-level panel from 1998 to 2004, when international adoptions peaked in the US, to estimate the effect of international adoptions and assisted reproductive technologies (ART) on adoption probabilities for foster children, focusing on infants and toddlers. Using granular data on the adopted children's country of origin and placement state, we identify the impact of international adoptions with a shift-share instrument. We find that international adoptions have significant and robust negative effects on foster adoptions, while the estimated effects of ART births are smaller and sensitive to model specification.
JEL Classifications: I38, J13, J18
Magnitude of Acute Otitis Media, Bacteriological Profile and Antimicrobial Sensitivity Patterns of Nasopharyngeal Isolates in Children Receiving Care in Moshi Municipality
Authors: Kayuza Michael P. , Mwakyoma Adam A. , Mlay Kenneth J. , Mtenga Philbert P. , Mchaile Deborah N. , Chussi Desderius C.
Published: November 2024

Background
Acute otitis media (AOM) is among the most common paediatric diseases and a leading cause of morbidity in children. Common etiologic pathogens include non-typable Haemophilus influenza, Streptococcus pneumoniae, Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa, Escherichia coli, Moraxella catarrhalis among others. Evidence shows an increase in cases of antimicrobial resistance when treating for AOM in children, which could be attributed to high rates of empirical treatment for cases of AOM without a clear microbial picture of the otopathogens responsible for the condition.
Aim
This study aimed at determining the magnitude of AOM as well as the antimicrobial sensitivity patterns of nasopharyngeal isolates in children with AOM receiving care at hospitals in Moshi Municipality
Methods
Multi-centre cross-sectional study, carried out between September 2023 to February 2024 at the 4 study sites in the outpatient departments. Children under the age of 5 years presenting with AOM during the study period were enrolled. An interview was conducted with parents/caregivers followed by clinical assessment of the children. Nasopharyngeal swabs were obtained from each of these patients and sent to the laboratory for bacterial culture and sensitivity. Data was analysed using SPSS v22.
Results
A total of 117 children were enrolled in the study from 420 children receiving care during the study period, making a magnitude of AOM was 27.9% with children between 13 to 24 months of age being the most susceptible. Common isolated pathogens included S. pyogenes, E. coli, Acinetobacter spp. and P. aeruginosa and S. aureus. Amikacin, Meropenem, Ciprofloxacin and Tazobactam/Piperacillin had the highest rates of susceptibility while Amoxicillin, Ampicillin, Ceftriaxone and Amoxicillin/Clavulanic acid showed high levels of resistance.
Conclusion
Magnitude of AOM among children is 27.9%. Bacterial pathogens isolated from nasopharyngeal swabs are different from the common etiologic bacteria seen in AOM which could be due to good vaccination coverage of PCV and Pentavalent. Non susceptibility to the commonly used antibiotics indicates the growing rate of antimicrobial resistance. Better pathogen-tailored therapy needs to be implemented to help curb this burden, as well as further investigations on the rising etiologic pathogen
Influence of grinding parameters on removal depth of 42CrMo steel and its prediction in robot electro-hydraulic-actuated abrasive belt grinding
Authors: Shi Dequan , Xu Youen , Zeng Xiongyawei , Zhang Huajun
Published: November 2024

The influences of revolution speed, feed speed, grinding force and grit designation on the material removal depth (MRD) and surface Vickers hardness of 42CrMo steel were investigated by using an adaptive electro-hydraulic-actuated triangular abrasive belt in robot grinding. A predictive model for MRD of 42CrMo steel has been established using the orthogonal central composite design method. The results indicated that as the revolution speed or grinding increases, both MRD and surface hardness increase. However, as the revolution speed surpasses 4000 RPM or the grinding force exceeds 60 N, the increase of MRD becomes slower due to the increase of surface hardness. Both the MRD and surface hardness decrease continuously as the feed speed increases, and once it exceeds 15 mm⸱s-1, the decrease of the MRD becomes slow. The rise in grit designation of abrasive belt makes the MRD reduces gradually while the surface hardness rises slightly. The correlation coefficient of the predictive model is 0.9387, and the relative error between the predicted and experimental MRD is within 10%, indicating a relatively high accuracy. At the optimal grinding parameters (grinding force of 81 N, revolution speed of 4739 RPM, and feed speed of 7.6 mm·s-1), the maximum MRD of 42CrMo steel achieved by an abrasive belt of 60 grit designation is 0.934 mm. This work provides a basis for high-precision robot abrasive belt grinding of 42CrMo steel.
Age-Related Changes in Brain Network Modularity Based on the Dynamic Sliding Window Subnetwork Voting Method
Authors: Liu Jianxi , Xia Nannan , Huang Mingcong , Linli Zeqiang
Published: November 2024

An understanding of the modular structure of brain functional networks and their changes with age is beneficial in uncovering the neural mechanisms that underlie cognitive decline during the ageing process. Compared to simpler networks, such as social networks, the execution of brain functions always depends on extensive interactions among multiple brain regions, which complicates the detection of accurate, stable, and physiologically meaningful community structures. However, although previous work has focused on the modular organization of the brain, there has been insufficient research on its specific dynamic changes and how these evolve with age. In this case, this paper investigates the modular structure of human brain functional networks and their dynamic changes across different age groups, revealing the impact of aging on brain network functionality. Firstly, we constructed brain networks using a dynamic sliding window subnetwork voting method. Further, this paper, based on public datasets and the Girvan-Newman (GN) community detection algorithm, effectively divided the community structure of brain networks and calculated modularity. It focused on analyzing the brain network characteristics of 439 participants under resting-state functional magnetic resonance imaging (fMRI) data. The results of the variance analysis indicate significant differences in modularity across different age groups. The brain networks of younger participants exhibited pronounced modular characteristics and higher efficiency in information processing. In contrast, older participants displayed a significant reduction in modularity, reflecting a trend towards functional integration. These changes are closely related to the decline in cognitive abilities and the degeneration of neural connections.
Heat and Mass Transfer in Ternary Nanofluids Flowing Over a Stretching Sheet in Porous Media: Effects of Thermal Buoyancy and Magnetic Fields
Authors: Chandrakala P. , Srinivasa V.
Published: November 2024

The effects of mass and heat transport in ternary nanofluids flowing across a stretching sheet through porous media are examined in this paper. Using convective boundary conditions, the analysis focused on incorporating the thermal buoyancy, magnetic field, thermal radiation, ananofluid comprises water-based Cu, Al2O3, and Ag nanoparticles. Using the appropriate transformations, the governing PDEs are transformed into nonlinear ODEs. It employs similarity transformations to convert governing partial differential equations into nonlinear ordinary differential equations, which are numerically solved using the bvp4c solver in MATLAB. This study offers a comparative analysis involving ternary (Cu + Al2O3 + Ag), binary (Cu + Al2O3) and monophasic (Cu) nanoparticle configurations suspended in water (H2O). The results revealed that the greater the dissipation coefficient (Ec) was, the greater the velocity and temperature and the lower the nanoconcentration. The surface drag, Nu and Sh decrease with increasing Ec. The lower the molecular diffusivity is, the lower the velocity, temperature, and concentration. This innovative study exploring the effects of heat and mass transfer in ternary nanofluids flowing through a porous medium over a stretching sheet has significant potential for application in various fields, such as biomedical engineering and renewable energy technologies, to increase the performance of solar collectors and geothermal systems through the improved heat transfer capabilities of nanofluids.
Foreign body aspiration in children: should we perform bronchoscopy on every patient?
Authors: Saraç Fatma , Yazıcı Mehmet
Published: November 2024

Purpose
Foreign body aspiration is an important health problem frequently seen in children and requiring emergency intervention due to severe morbidity and mortality. This study evaluates patients who underwent bronchoscopy with diagnoses of foreign body aspiration.
Methods
All children aged under 18 who underwent bronchoscopy with diagnoses of foreign body aspiration between June 2020 and December 2023 were included in the study. The patients’ medical records were examined retrospectively.
Results
Three hundred ten (310) patients aged between three months and 15 years underwent bronchoscopy due to suspected foreign body aspiration. 83% of the patients were aged two or younger, and boys outnumbered girls two to one. Foreign bodies were detected at bronchoscopy in 202 (65.16%) patients. Foreign bodies were most frequently located in the right main bronchus (47.52%). In addition, 83.66% of the foreign bodies consisted of organic material. The most frequently aspirated material was peanut (32.54%).
Conclusion
No symptom, finding, or test is definitively diagnostic or exclusive in patients presenting with suspected foreign body aspiration. Despite a negative bronchoscopy rate of 34.83% in this study, since we observed no severe complications or mortality we recommend that it be performed on all patients with suspected foreign body aspiration.
A Multi-Omic Analysis to Investigate the Causal Associations Between Circulating Proteins and Risk of Spontaneous Abortion and Their Potential Implications
Authors: Huang Min , He Mei-hua , Xiang Jia-hui , Zhang Li-mei , Liu Ying-hui , Sun Xiao-li
Published: November 2024

Background
Spontaneous abortion is a complex disorder with a significant genetic component. Identifying genetic variants influencing spontaneous abortion risk could unveil biological pathways and potential therapeutic targets.
Methods
We performed Mendelian randomization using cis- and trans-protein quantitative trait loci (pQTLs) as instrumental variables to assess causal effects of circulating proteins on spontaneous abortion. Proteins exhibiting differential expression between sexes were excluded. KEGG pathway enrichment was employed to investigate the pathways affected by susceptibility genes, while single-cell transcriptomic analysis was utilized to explore the susceptible cell types with elevated expression of these genes within the uterine endometrium.
Results
MMP9 and DC-SIGN were associated with increased spontaneous abortion risk (OR = 1.11(1.03–1.19), P = 3.70x10− 3; OR = 1.09(1.02–1.16), P = 9.89x10− 3), while HBAZ and NELL1 had protective effects (OR = 0.96(0.94–0.99), P = 5.20x10− 3; OR = 0.94(0.9–0.98), P = 8.54x10− 3). Additionally, TMM85 conferred higher spontaneous abortion risk (OR = 1.06(1.02–1.1), P = 4.72x10− 3). Pathway analysis highlighted sphingolipid binding, chemorepellent activity, and tumor necrosis factor receptor activity. Single-cell transcriptomics revealed that MUL1, EMC4, NDC80, and SELL gene exhibit higher expression levels within uterus cells, and these susceptibility genes displayed elevated expression levels in leukocytes, mature NK T cells, and T cells in uterus.
Conclusion
Our integrated multi-omics analysis identified genetic variants influencing spontaneous abortion risk and their downstream molecular mechanisms, providing insights into potential therapeutic targets. The implicated pathways and cell types may guide future investigations into the pathogenesis of spontaneous abortion.
“Sonography findings spurring suspicion for a confirmed case of Leiomyosarcoma: A Case Report”
Authors: Adejuyigbe Temiloluwa , Burgess Anh Vi , Nguyen Brandon , Andrews Brooke , Salazar Christina , WIlliams-Brown Marian Yvette
Published: November 2024

Background: Differentiating degenerating leiomyomas and malignant leiomyosarcomas is often difficult, given the lack of criteria for imaging findings and difficulty in reliably sampling myometrial neoplasms of the uterus.
Case: We present the case of using a transvaginal ultrasound to evaluate for characteristics of leiomyosarcoma prior to further referral of a patient to gynecology oncology for hysterectomy, with final pathology confirming a leiomyosarcoma.
Conclusions: Ultrasound findings of uterine masses that include a large mass with heterogeneous tissue, irregular loculated spaces in combination with increased vascularity and symptoms of pelvic pain and abnormal uterine bleeding, as described in this case, can support a suspicion of and further investigation for malignancy. In addition, further evaluation of ultrasound as a modality to evaluate vascularity in atypical uterine masses is supported by this presentation
Vision Transformer-based Meta Loss Landscape Exploration with Actor-Critic Method
Authors: Zhang Enzhi , Zhong Rui , Du Xingbang , Wahib Mohamed
Published: November 2024

Detecting and mitigating overfitting in deep neural networks remains a critical challenge in modern machine learning. This paper investigates innovative approaches to address these challenges, particularly focusing on vision transformer-based models. By leveraging meta-learning techniques and reinforcement learning frameworks, we introduce Transformer-based Loss Landscape Exploration (TLLE), which utilizes the validation loss landscape to guide gradient descent optimization. Unlike conventional methods, TLLE employs the Actor-Critic algorithm to learn the mapping from model weights to future values, facilitating efficient sample collection and precise value predictions. Experimental results demonstrate the superior performance of TLLE-enhanced transformer models in image classification and segmentation tasks, showcasing the efficacy of our approach in optimizing deep learning models for image analysis.
Assessment of Dry Weight in Hemodialysis Patients by Bioelectrical Impedance Analysis
Authors: Zeng Xiaoqing , Cheng Yi , Chen Lin , Hu Wen , Liu Yuan
Published: November 2024

Background: Diabetes and hypoalbuminemia are commonly observed in hemodialysis patients, which affect assessment of dry weight.
Methods: the patients with higher Kt/V than 1.2, were divided into group A (- -), group B (- +), group C (+ -) and group D (+ +) according to whether they had diabetes and hypoalbuminemia. And they were measured the body composition by bioelectrical impedance analysis (BIA). And validation studies were conducted.
Results: The ECW/TBW and ECW/ICW of group D was significantly higher than those of group A, group B, and group C (p＜0.05). Additionally, The ECW/ICW and ECW/TBW of groups B and C was significantly higher than that of group A (p＜0.05). The Kappa value for the evaluation of dialysis adequacy using the two methods was 0.532 (0.409,0.601, p<0.001).
Conclusions: The BIA method can be utilized to measure the body composition of hemodialysis patients for evaluating dry body weight. It is potential to provide objective data through calculation to determine the ultrafiltration volume, in order to reduce volume load and improve the quality of life of hemodialysis patients.
Ion-Molecule Reaction Kinetics of C2H+ and COH+ with a permanent dipole NH3 molecule
Authors: Silva Washington B. da , Albernaz Alessandra F. , Correa Eberth
Published: November 2024

In this work, the ab initio potential energy surfaces were calculated for the COH+ + NH3 and C2H+ + NH3 reactions. To calculate the rate constants we employed three different methods: Master Equation Solver for Multi-Energy Well Reactions , and two well established capture theories, ADO and AADO. The last ones were revised from the original paper by Su et al.[1], and the revised equations can be found in our Appendix. To obtain the respective rate constants through the ADO and AADO theories we propose an analytical approximation for the relation between the relative velocity, v, of the reactants and the critical impact parameter, bc in the capture theory used for ADO and AADO. The resulting rate constants in the interval from 200 K to 1000 K were obtained and compared in different theories.
Tanshinone IIA targets RNF123 to inhibit non-small cell lung cancer progression via KAT2B-mediated H3K18ac modification
Authors: He Yongjie , Zhang Yingxin , Zhang Jiangsheng , Cai Kaican
Published: November 2024

Background
Tanshinone IIA (TSIIA) presents excellent anti-tumor effect. Our
work aims to explore the pharmacological effect of TSIIA on lung
cancer development and define the underlying mechanism.

Methods
Cell viability and proliferation were assessed using CCK-8 and
EdU assays, respectively. Transwell assay was adopted to determine
cell migration and invasion. H3K18ac and H4K8ac enrichment in the
RING finger protein 123 (RNF123) promoter and the interaction
between lysine acetyltransferase2B (KAT2B) and RNF123 promoter were
analyzed by ChIP assay.

Results
RNF123 is weakly expressed in lung cancer, and its upregulation
represses lung cancer cell viability, proliferation, migration,
invasion, and epithelial-mesenchymal transition (EMT). Sodium
tanshinone IIA sulfate (STS) treatment inhibits non-small cell lung
cancer cell malignant phenotypes, which are reversed by the
knockdown of RNF123. Mechanistically, TSIIA promotes KAT2B-mediated
H3K18ac modification of RNF123 and RNF123 expression in non-small
cell lung cancer cells. KAT2B knockdown prevents STS-induced
inhibition on non-small cell lung cancer cell malignant phenotypes.
RNF123 upregulation restores the effect of KAT2B knockdown on
STS-treated non-small cell lung cancer cell malignant
phenotypes.

Conclusion
TSIIA inhibits non-small cell lung cancer cell malignant
phenotypes via epigenetic modification of RNF123.
WITHDRAWN: Resveratrol alleviates testicular toxicity induced by anti-PD-1 through regulating the NRF2-SLC7A11-GPX4 pathway
Authors: Tuerxun Halahati , Zhao Yixin , Li Yawen , Liu Xingyu , Wen Shuhui , Zhao Yuguang
Published: November 2024

The full text of this preprint has been withdrawn by the authors while they make corrections to the work. Therefore, the authors do not wish this work to be cited as a reference. Questions should be directed to the corresponding author.
Principal Component Analysis of Spatial Water Quality Variation in Shallow Wells of Half London Ward, Tanzania
Authors: William Matungwa , Katambara Zacharia
Published: November 2024

Groundwater is a crucial water source for urban areas in Africa, particularly where surface water is insufficient to meet demand. This study analyzes the water quality of five shallow wells (WW1-WW5) in Half-London Ward, Tunduma Town, Tanzania, using Principal Component Analysis (PCA) to identify the primary factors influencing groundwater contamination. Monthly samples were collected over 12 months and analysed for physical, chemical, and biological parameters. The PCA revealed between four and six principal components (PCs) for each well, explaining between 84.61% and 92.55% of the total variance in water quality data. In WW1, five PCs captured 87.53% of the variability, with PC1 (33.05%) dominated by pH, EC, TDS, and microbial contamination, suggesting significant influences from surface runoff and pit latrines. In WW2, six PCs explained 92.55% of the variance, with PC1 (36.17%) highlighting the effects of salinity, TDS, and agricultural runoff. WW3 had four PCs explaining 84.61% of the variance, with PC1 (39.63%) showing high contributions from pH, hardness, and salinity, indicating geological influences and contamination from human activities. Similarly, in WW4, six PCs explained 90.83% of the variance, where PC1 (43.53%) revealed contamination from pit latrines and fertilizers. WW5 also had six PCs, accounting for 92.51% of the variance, with PC1 (42.73%) indicating significant contamination from agricultural runoff and pit latrines. The study concludes that groundwater quality in Half-London Ward is primarily affected by a combination of surface runoff, pit latrine contamination, agricultural inputs, and geological factors. The presence of microbial contaminants and elevated nitrate and phosphate levels underscores the need for improved sanitation and sustainable agricultural practices. Recommendations include strengthening sanitation infrastructure, promoting responsible farming techniques, and implementing regular groundwater monitoring to safeguard water resources and public health in the region.
The perils of premature evaluation: reassessing the application of Benford’s Law to the USA’s COVID-19 data
Authors: Dutta-Powell Ravi
Published: November 2024

This paper reviews earlier applications that claimed non-conformity of COVID-19 in the United States with Benford's Law, and uses later and more granular data to demonstrate that this was likely due to the data being unsuitable for applications of Benford’s Law. It also demonstrates that the same dataset, analysed in different ways, can show vastly different levels of conformity with Benford’s Law. Specifically, most US states show high degrees of conformity for COVID-19 cases and cumulative deaths when the Robust Order of Magnitude (ROM) is over three and data at the county level is used to analyse state outcomes. Conversely, when county data is aggregated to the state level and analysed (i.e., case totals for all counties are summed to create a single state figure for each day of the pandemic), every state shows nonconformity. Only new deaths showed the reverse pattern - this is likely because new deaths at the county level do not span sufficient orders of magnitude, and aggregation to the state level overcomes this. This suggests that some instances of non-conformity with Benford’s Law in the literature may be driven by applications to inappropriate datasets or methodological issues.
Towards explicit soft skills labelling in ESCO through semantic NLP analysis
Authors: Panzaru Ciprian , Grama Anamaria
Published: November 2024

The classification and identification of soft skills within the European Skills, Competences, Qualifications, and Occupations (ESCO) framework presents a significant challenge, as ESCO does not explicitly indicate which of its listed skills are considered “soft”. This lack of clear labelling complicates the strategic alignment of educational programs and workforce development initiatives with the evolving demands of today’s labour market, where soft skills are increasingly recognised as essential for professional success. Our study seeks to address this challenge by conducting a systematic literature review to identify and define a comprehensive set of soft skills, and by employing Natural Language Processing (NLP) techniques to map these skills to the ESCO taxonomy. Through semantic analysis and comparison of soft skills identified from scientific literature against the ESCO skillset, we establish a refined classification that explicitly labels soft skills within the ESCO framework. Our findings provide a necessary clarification of soft skills within the standardised European context, offering valuable insights for policymakers, educators, and HR professionals focused on fostering soft skills development. By clarifying the soft skills embedded within the ESCO framework, this research contributes to the advancement of skill taxonomies and promotes a more integrated approach to the development and assessment of soft skills.
Calcium and Nitrogen Fertilization of Alstroemeria for Cut Flower Production
Authors: Smith Mark A. , Elliott George C. , Bridgen Mark P.
Published: November 2024

The effects of Ca and N on cut flower production of Alstroemeria were determined in separate greenhouse experiments. Calcium was supplied as Ca(NO3)2 and CaCl2 at 0, 1, 2, 4, 8, and 12 mmol·L-1 added to tap water containing Ca at ≈0.2 mmol·L-1. Nitrogen was supplied as KNO3 and Ca(NO3)2 providing total N at 0, 3.5, 7, 14, 28.5, and 57 mmol·L-1 in tap water containing N <0.2 mmol·L-1. Nutrient solutions were applied at 7- or 10-day intervals to plants growing in a soilless medium in 2.6- or 5.5-L containers. Flowering stems were harvested when the primary florets opened. Total N concentration was measured in leaf tissue from the upper portion of flowering stems. Flower production was not affected by Ca supply, but increased with N supply to a maximum of about four stems per plant on a weekly basis at 28.5 mmol·L-1, then decreased to less than three stems per plant at 57 mmol·L-1. Nitrogen concentration in leaf tissue on a dry mass basis was maintained at 45 ±3 g·kg-1 in plants supplied with N at 28.5 mmol·L-1, 52±5 g·kg-1 at 57 mmol·L-1, but <40 g·kg-1 with N supply of 14 mmol·L-1 or lower. Nitrogen fertilization of Alstroemeria should be managed to maintain leaf tissue N close to 45 g·kg-1.
Effect of Uniconazole on Shoot Growth and Budset of Containerized Fraser Fir
Authors: Hinesley L. Eric , Warren Stuart L. , Snelling Layne K.
Published: November 2024

In two experiments, uniconazole (0.25 to 16 mg·L-1 a.i.) was applied as a root drench to containerized Fraser fir [Abies fraseri (Pursh) Poir.] at various times of the year. Leader length, stem diameter, length of laterals, and number of subterminal buds were reduced the following growing season. Treatment during the 1994 growing season reduced lateral bud formation on the leader in 1995, whereas treatment with 8 or 16 mg·L-1 in Mar. 1995 (prior to budbreak) increased it. Uniconazole caused needle discoloration and abscission at concentrations ≥4 mg·L-1. Leader growth was reduced more than branch elongation, which tended to make plants more decurrent. The utility of uniconazole in production of tabletop Fraser fir Christmas trees was unclear; reduced shoot elongation was often accompanied by fewer lateral buds and needle discoloration and/or abscission. Chemical name used: E-1-(p-Chlorophenyl)-4,4-dimethyl-2-(1,2,4-triazole-1-penten-3-ol) (uniconazole).
ARQUITETURA ESCOLAR: UMA ANÁLISE SOBRE AS DIRETRIZES PROJETUAIS DO FNDE
Authors: Mendonça Jean Carlos Passamai , Rocha Letícia Maria Andrião , Vial Rafael Matos
Published: November 2024

A presente pesquisa trata da importância do ambiente físico escolar no processo de ensino/aprendizagem diante das premissas e objetivos da educação. O Ministério da Educação (MEC), através do Fundo Nacional de Desenvolvimento da Educação (FNDE) elaborou uma série de publicações com objetivo de suporte técnico para elaboração de projetos educacionais. Entre eles o Manual de Orientações Técnicas para Elaboração de Projetos de Edificações Escolares do Ensino Fundamental estabelece diretrizes projetuais fundamentais em consonância com a concepção contemporânea de arquitetura escolar. O objetivo desta pesquisa é estabelecer um panorama comparativo entre o espaço físico escolar de três escolas públicas de ensino fundamental da cidade de Cachoeiro de Itapemirim – Espírito Santo e o atendimento quanto ao que determina atualmente o Manual do FNDE mediante análise de seus espaços físicos, verificando a porcentagem de atendimento e se os resultados são satisfatórios em relação ao mínimo exigido. A metodologia empregou análise empírica e dos projetos arquitetônicos fornecidos pela Secretaria Municipal de Educação, bem como registros fotográficos de visitas in loco. Os levantamentos de dados foram feitos através de visitas in loco e registros fotográficos para verificação quanto à fidelidade ao projeto fornecido pela Secretaria Municipal de Educação. Após os levantamentos e análises realizadas, foram identificados os percentuais mínimos de cumprimento das diretrizes com o intuito de concluir se o ambiente físico escolar analisado está em consonância com as diretrizes projetuais estabelecidas pelo Manual de Orientações Técnicas do FNDE. O resultado obtido demonstra necessidade de diversas adequações nos ambientes físicos escolares para contribuição na promoção de uma educação de qualidade através de uma concepção contemporânea de arquitetura escolar.
Character-Based Digital Curriculum and Learning: A Case Study in Madrasah Ibtidaiyah and Elementary Schools
Authors: Kulsum Umi , Bahrissalim Bahrissalim , Khadijah Siti , Fauzan Fauzan , Arifin Fatkhul
Published: November 2024

This study examines the curriculum and learning processes implemented at Madrasah Ibtidaiyah Pembangunan and Pembangunan Islamic Elementary School, focusing on integrating character values. Utilizing a qualitative research methodology, the investigation collects data through in-depth interviews, direct observations of classroom activities, and analyses of relevant documents, all aimed at gaining a deeper understanding of the educational practices at these institutions. The findings indicate that both Madrasah Ibtidaiyah Pembangunan and Bina Insani Elementary School exhibit distinctive learning characteristics that set them apart from other Islamic educational institutions. The academic policies at these schools have successfully embraced digital learning models that are closely aligned with character education. This approach effectively fosters essential character values among students, including a heightened sense of responsibility and a strong discipline regarding the appropriate use of information technology. The results suggest that the integration of digital learning with character education can substantially enhance the overall development of students within Islamic educational environments.
EXPERIÊNCIAS EM ESTÁGIO NA EDUCAÇÃO INFANTIL
Authors: Almeida Galvão Cauê , Oliveira Medeiros Sâmia Lorena , Silveira de Medeiros João Paulo
Published: November 2024

Este trabalho se trata de um relato de experiência articulado por dois estudantes com apoio de um orientador, na construção e consolidação das atividades de regência em sala de aula. Sabe-se que o estágio na educação infantil é uma experiência prática fundamental para futuros educadores, proporcionando a oportunidade de aplicar teorias pedagógicas em um ambiente real. Além disso, os estagiários aprendem a trabalhar em equipe com outros educadores e a comunicar-se efetivamente com as famílias. Essa vivência contribui para a formação de profissionais mais preparados, capacitando-os a lidar com os desafios da educação infantil e a fomentar o desenvolvimento integral das crianças. Dessa forma, ao relatarmos nossas experiências de estágio esperamos colaborar com a ampliação do estágio e dos impactos possíveis para uma formação docente voltada à prática que favoreça o não abandono da profissão após a formação inicial em pedagogia.
Validity of a Consumer-Based Wearable to Measure Clinical Parameters in Patients With Chronic Obstructive Pulmonary Disease and Healthy Controls: Observational Study
Authors: Hermans Fien , Arents Eva , Blondeel Astrid , Janssens Wim , Cardinaels Nina , Calders Patrick , Troosters Thierry , Derom Eric , Demeyer Heleen
Published: November 2024

Background

Consumer-based wearables are becoming more popular and provide opportunities to track individual’s clinical parameters remotely. However, literature about their criterion and known-groups validity is scarce.

Objective

This study aimed to assess the validity of the Fitbit Charge 4, a wrist-worn consumer-based wearable, to measure clinical parameters (ie, daily step count, resting heart rate [RHR], heart rate variability [HRV], respiratory rate [RR], and oxygen saturation) in patients with chronic obstructive pulmonary disease (COPD) and healthy controls in free-living conditions in Belgium by comparing it with medical-grade devices.

Methods

Participants wore the Fitbit Charge 4 along with three medical-grade devices: (1) Dynaport MoveMonitor for 7 days, retrieving daily step count; (2) Polar H10 for 5 days, retrieving RHR, HRV, and RR; and (3) Nonin WristOX2 3150 for 4 nights, retrieving oxygen saturation. Criterion validity was assessed by investigating the agreement between day-by-day measures of the Fitbit Charge 4 and the corresponding reference devices. Known-groups validity was assessed by comparing patients with COPD and healthy controls.

Results

Data of 30 patients with COPD and 25 age- and gender-matched healthy controls resulted in good agreement between the Fitbit Charge 4 and the corresponding reference device for measuring daily step count (intraclass correlation coefficient [ICC2,1]=0.79 and ICC2,1=0.85, respectively), RHR (ICC2,1=0.80 and ICC2,1=0.79, respectively), and RR (ICC2,1=0.84 and ICC2,1=0.77, respectively). The agreement for HRV was moderate (healthy controls: ICC2,1=0.69) to strong (COPD: ICC2,1=0.87). The agreement in measuring oxygen saturation in patients with COPD was poor (ICC2,1=0.32). The Fitbit device overestimated the daily step count and underestimated HRV in both groups. While RHR and RR were overestimated in healthy controls, no difference was observed in patients with COPD. Oxygen saturation was overestimated in patients with COPD. The Fitbit Charge 4 detected significant differences in daily step count, RHR, and RR between patients with COPD and healthy controls, similar to those identified by the reference devices, supporting known-groups validity.

Conclusions

Although the Fitbit Charge 4 shows mainly moderate to good agreement, measures of clinical parameters deviated from the reference devices, indicating that monitoring patients remotely and interpreting parameters requires caution. Differences in clinical parameters between patients with COPD and healthy controls that were measured by the reference devices were all detected by the Fitbit Charge 4.
Ethical Dilemmas Among Oncology Nurses in China: Cross-Sectional Study
Authors: Ko Eunjeong , Shamsalizadeh Neda , Lee Jaehoon , Ni Ping
Published: November 2024

Background

Effective communication about cancer prognosis is imperative for enhancing the quality of end-of-life care and improving patient well-being. This practice is sensitive and is heavily influenced by cultural values, beliefs, and norms, which can lead to ethical dilemmas. Despite their significance, ethical challenges in nursing related to prognosis communication are understudied in China.

Objective

This study aimed to examine the ethical dilemmas relating to cancer prognosis communication and their associated factors.

Methods

A cross-sectional design was employed to survey 373 oncology nurses in mainland China. Data were collected on ethical dilemmas, attitudes, barriers, experiences with prognosis communication, sociodemographics, and practice-related information. Ordinary least squares regressions were used to identify factors contributing to ethical dilemmas.

Results

Participants reported a moderate level of ethical dilemmas in prognostic communication (mean 13.5, SD 3.42; range 5‐20). Significant predictors of these dilemmas included perceived barriers (P<.001), experiences with prognosis communication (P<.001), and years of work experience (P=.002). Nurses who perceived greater communication barriers, had more negative experiences with prognosis communication, and had less work experience were more likely to encounter ethical dilemmas in prognosis-related communication.

Conclusions

Chinese oncology nurses frequently encounter ethical dilemmas, as well as barriers, in communicating cancer prognoses. This study’s findings emphasize the importance of culturally tailored communication training. Collaborative interprofessional training, particularly through physician-nurse partnerships, can perhaps enhance the proficiency of cancer prognosis-related communication.