bluuebunny/crossref_metadata_2025 huggingface dataset:
Is there sufficient evidence to support the health benefits of including donkey milk in the diet?
Authors: Khan Muhammad Zahoor , Chen Wenting , Li Mengmeng , Ren Wei , Huang Bingjian , Kou Xiyan , Ullah Qudrat , Wei Lin , Wang Tongtong , Khan Adnan , Zhang Zhenwei , Li Liangliang , Wang Changfa
Published: September 2024

Donkey milk has attracted attention due to its distinctive nutritional composition and potential health advantages, particularly because of its whey protein content, which includes lysozyme, α-lactalbumin, lactoferrin, and β-lactoglobulin and vitamin C, among other components. These elements contribute to immunoregulatory, antimicrobial, antioxidant, and anti-inflammatory properties, positioning donkey milk as a possible therapeutic option. In addition, due to the low levels of caseins, the casein-to-whey protein ratio, and the β-lactoglobulin content in donkey milk, it presents an optimal alternative for infant formula for individuals with cow’s milk allergies. Moreover, research into donkey milk’s potential for cancer prevention, diabetes management, and as a treatment for various diseases is ongoing, thanks to its bioactive peptides and components. Nevertheless, challenges such as its low production yield and the not fully understood mechanisms behind its potential therapeutic role necessitate more thorough investigation. This review consolidates the existing knowledge on the therapeutic possibilities of donkey milk, emphasizing its importance for human health and the need for more detailed studies to confirm its health benefits.
Hypovitaminosis D in university workers in Southern Ecuador: interactions between gender and lifestyle
Authors: Díaz Patricia , Cadena Marcela , Montalván Martha Elena , Garrochamba Kleber , Calderón Paula , Carrión Gloria , Santana Sergio
Published: September 2024

Background

Hypovitaminosis D may be common in tropical countries and is linked to disorders of phospho-calcium metabolism, rickets, muscle pain, immune system deficiencies, and increased susceptibility to microbial infections.

Objective

To assess the prevalence of hypovitaminosis D in apparently healthy university workers in Loja, Ecuador.

Methods

A cross-sectional study was completed in a private Ecuadorian university from May 2023 to September 2023, involving 440 participants. Data were gathered using a structured questionnaire created to assess risk factors influencing vitamin D levels. Serum 25-hydroxyvitamin D (25-OH)D concentrations were measured utilizing immunoenzymatic methods. Altered states (insufficiency or deficiency) of vitamin D were defined with serum values <30 ng/mL. Associations between vitamin D status and selected determinants were analyzed with independence tests, with significance set at p < 0.05. Where possible, odds ratios (OR) were calculated using logistic regression.

Results

The sample consisted of 60.9% faculty members and 39.1% administrative staff; 42.7% were men and 57.3% were women, with an average age of 41.9 ± 7.6 years. Only 2.7% of participants were aged 60 years or older. The mean serum 25-(OH)D concentration was 19.5 ± 6.8 ng/mL. Altered 25-(OH)D levels were found in 93.4% of participants, with 94.0% showing decreased serum 25-(OH)D concentrations and 1.6% displaying deficiency states. Hypovitaminosis D was associated with sex (OR = 2.40; 95% CI: 1.3–5.57; p < 0.05) and sunscreen use (OR = 0.36; 95% IC: 0.13–0.99; p < 0.05).

Conclusion

Hypovitaminosis D was almost universal among the apparently healthy university workers studied. The findings suggest that both sex and sunscreen use may independently or jointly contribute to hypovitaminosis D in these individuals. Further studies will be required to clarify this interplay.
Management of jellyfish outbreaks to achieve good environmental status
Authors: Sagarminaga Yolanda , Piraino Stefano , Lynam Christopher P. , Leoni Valentina , Nikolaou Athanasios , Jaspers Cornelia , Bosch-Belmar Mar , Fumarola Lara M. , Borja Ángel , Spada Emanuela , Amorim Eva , Borrello Patrizia , de Angelis Roberta , Leone Antonella , Montero Natalia , Ferrer Luis , Holland Matthew M. , Doyle Thomas K. , Tsirtsis George , Katsanevakis Stelios
Published: September 2024

Jellyfish and gelatinous zooplankton (GZ) in general, fulfill important ecological roles with significant impacts, although they are often oversimplified or misunderstood. This paper reviews the impacts, pressures, monitoring methods and current management strategies for various GZ groups. It also introduces potentially applicable indicators for their assessment in ecosystem-based management approaches, such as the European Marine Strategy Framework Directive (MSFD). This multi-faceted review is primarily envisioned to serve as a state-of-the-art document for scientists and policymakers to foster a holistic assessment and management of GZ across European regional seas. The systematic review on global impacts of GZ shows a notable increase in the number of studies since the early 2000s. Stings were the main cause of human health impacts. Mechanisms that impact biodiversity included direct predation, modification of trophic flows or competition for resources. Several GZ taxa may be beneficial to biodiversity acting as biological regulators and provide societal ecosystem services such as food provision or medical applications. The systematic review on monitoring techniques outlined a variety of methods, such as nets (the most common technique), continuous plankton recorder (CPR), polyp and jelly-fall monitoring, acoustic methods, remote aerial and underwater imaging, molecular methods, and citizen science. Furthermore, several currently employed management strategies were enumerated, including the use of anti-jelly nets, bubble curtains, chemical compounds, or the introduction of GZ predators. This study highlights the pressing need for enhanced GZ-dedicated monitoring, assessment, and anticipatory management of GZ populations to address future GZ crises more effectively and cost-efficiently. Moreover, exploring GZ ecosystem services unveils opportunities to harness marine resources while mitigating adverse effects, thereby supporting sustainable blue economies.
Effect of the gut microbiome and inflammation-related proteins on oral leukoplakia: a Mendelian randomization study and mediation analysis
Authors: Da Junlong , Ren Yinting , Liu Shiwei , Wang Nanyan , Wang Lei , Fu Zhifeng , Liang Yongtang , Pan Yu , Li Jin , Chen Jufeng
Published: September 2024

Background

Oral leukoplakia (OL) is the most common potentially malignant disease of the oral cavity. In recent years, studies have identified a correlation between the gut microbiota (GM) and oral cancer, in addition, inflammation-related proteins have been reported to play an important role in the development of OL. However, the causal relationship between gut microbiota and OL, as well as whether cytokines play a mediating role, remain unclear.

Methods

In this Mendelian randomization (MR) study, the genome-wide association studies (GWAS) (n=18340) of the MiBioGen consortium microbiome was used as exposure data. Genetic variation data related to OL were extracted from the Finngen R9 project (513 cases of OL and 411668 controls). The 91 inflammation-related proteins obtained in the literature serve as potential mediators. Two-sample Mendelian randomization analysis was applied to infer causality using Inverse Variance Weighted (IVW), MR Egger, weighted media, simple mode and weighted mode method. Subsequently, sensitivity analysis was conducted to ensure the robustness of the MR results. In addition, we conducted reverse MR analysis to alleviate reverse causality. Finally, we used mediation analysis to determine the pathway mediated by inflammation-related proteins from the gut microbiota to OL.

Results

The five bacterial taxa in the gut microbiota indicate a potential causal relationship with the development of OL. Notably, family Clostridiaceae1 was negatively correlated with the risk of OL development, while genus Dorea, genus Ruminococcus1, genus Senegalimasilia and genus Veillonella were positively associated with the risk of OL development. In addition, this study identified a potential causal relationship between interleukin-10 receptor subunit alpha (IL-10RA), interleukin-18 receptor 1(IL18-R1) and the occurrence of OL. In addition, intermediary analysis indicates that IL18-R1 mediated the pathway between the gut microbiota genus Senegalimasilia and OL.

Conclusions

In summary, our research emphasize the complex relationship between gut microbiota, inflammation-related proteins and OL. The identified associations and mediating effects provide new insights into potential therapeutic approaches for targeting the gut microbiota in the management of OL, and contribute to its prevention, diagnosis and treatment.
Long non-coding RNA FAM87A is associated with overall survival and promotes cell migration and invasion in gastric cancer
Authors: Jiang Xue , Wu Xiaobin , Lu Manjiao , Fan Wenna , Song Jing , Song Fangzhou
Published: September 2024

Background

The role of long non-coding RNAs (lncRNAs) in the invasion and metastasis of gastric cancer remains largely unclear.

Methods

Integrating transcriptome data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases, differentially expressed genes were identified in gastric cancer. Using the Catalogue of Somatic Mutations in Cancer (COSMIC) database-curated gene set, lncRNAs associated with invasion and metastasis were identified. The Cox analyses were performed to identify prognostic lncRNAs. The competing endogenous RNA (ceRNA) regulation network was constructed to identify hub lncRNAs in gastric cancer. Functional and pathway analyses were used to investigate the function of identified lncRNAs. RT-qPCR and Transwell assays were used to investigate the expression in gastric cancer tissues and functions in gastric cancer cell lines.

Results

Based on GEO and TCGA databases, 111 differentially expressed lncRNAs were identified between gastric cancer and normal samples. A total of 43 lncRNAs were significantly correlated with hallmark genes of cancer invasion and metastasis. Among them, as a hub lncRNA in the invasion-related ceRNA regulation network, FAM87A showed potential regulation on MAPK signaling and transforming growth factor (TGF) signaling cascade, such as TGFB2, TGFBR1, and TGFBR2. Furthermore, FAM87A also showed a significant correlation with cell adhesion molecules, such as Integrin alpha 6 (ITGA6) and Contactin-1 (CNTN1). RT-qPCR experiments showed that FAM87A expression was upregulated in gastric cancer tissues compared to normal samples (n = 30). Transwell assays showed that FAM87A knockdown inhibited the migration and invasion abilities of gastric cancer cells in vitro. Notably, clinical data analysis showed that lncRNA FAM87A could be an independent factor for the overall survival of patients with gastric cancer.

Conclusion

LncRNA FAM87A may play a pivotal role in regulating migration and invasion of gastric cancer cells. FAM87A could be a potential biomarker for the overall survival of patients with gastric cancer.
Mapping the evolution and research landscape of ferroptosis-targeted nanomedicine: insights from a scientometric analysis
Authors: Cao Siyang , Wei Yihao , Yue Yaohang , Wang Deli , Yang Jun , Xiong Ao , Zeng Hui
Published: September 2024

Objective

Notable progress has been made in “ferroptosis-based nano drug delivery systems (NDDSs)” over the past 11 years. Despite the ongoing absence of a comprehensive scientometric overview and up-to-date scientific mapping research, especially regarding the evolution, critical research pathways, current research landscape, central investigative themes, and future directions.

Methods

Data ranging from 1 January 2012, to 30 November 2023, were obtained from the Web of Science database. A variety of advanced analytical tools were employed for detailed scientometric and visual analyses.

Results

The results show that China significantly led the field, contributing 82.09% of the total publications, thereby largely shaping the research domain. Chen Yu emerged as the most productive author in this field. Notably, the journal ACS Nano had the greatest number of relevant publications. The study identified liver neoplasms, pancreatic neoplasms, gliomas, neoplasm metastases, and melanomas as the top five crucial disorders in this research area.

Conclusion

This research provides a comprehensive scientometric assessment, enhancing our understanding of NDDSs focused on ferroptosis. Consequently, it enables rapid access to essential information and facilitates the extraction of novel ideas in the field of ferroptotic nanomedicine for both experienced and emerging researchers.
Outdoor large-scene 3D point cloud reconstruction based on transformer
Authors: Tang Fangzhou , Zhang Shuting , Zhu Bocheng , Sun Junren
Published: September 2024

3D point clouds collected by low-channel light detection and ranging (LiDAR) are relatively sparse compared to high-channel LiDAR, which is considered costly. To address this, an outdoor large-scene point cloud reconstruction (LSPCR) technique based on transformer is proposed in this study. The LSPCR approach first projects the original sparse 3D point cloud onto a 2D range image; then, it enhances the resolution in the vertical direction of the 2D range image before converting the high-resolution range image back to a 3D point cloud as the final reconstructed point cloud data. Experiments were performed on the real-world KITTI dataset, and the results show that LSPCR achieves an average accuracy improvement of over 60% compared to non-deep-learning algorithms; it also achieves better performance compared to the latest deep-learning algorithms. Therefore, LSPCR is an effective solution for sparse point cloud reconstruction and addresses the challenges associated with high-resolution LiDAR point clouds.
How context influences the processing of relevant information and judgment accuracy—the role of information restriction in judgment processes in diagnosing misconceptions
Authors: Rieu Andreas , Loibl Katharina , Leuders Timo
Published: September 2024

To adapt teaching to the prerequisites of students, teachers have various options at their disposal to gather and process information as the basis to form a judgment, such as carrying out tests, talking to and observing the behavior of students, or administering tasks. The complexity of such a judgment arises from the multitude of observations and their different possible explanations. This complexity might be reduced when teachers focus on one hypothesis instead of considering multiple hypotheses, interpret information in a confirmatory way, and not collect diagnostically relevant information. However, in this way, they run the risk of undesirable biased judgments. It therefore seems important to improve diagnostic judgments by selecting and processing information in a more reflective way. Research indicates that if information on a student is not easily available but restricted (e.g., by time pressure, difficult access to the student or high effort), a teacher who wants to make a careful decision is forced to rely on more reflective processes in the selection of tasks and in the interpretation of solutions. The present experimental study therefore investigates how the restricted availability of information in a specific diagnostic situation—when diagnostically inexperienced prospective mathematics teachers determine misconceptions in decimal fractions—influences the underlying cognitive processes. We assume that restricting the availability of information on student behavior augments the attentional focus and therefore reduces cognitive biases. Such more reflective processing can be observed by an increased time spent per piece of information, which should lead to the processing of relevant information and further increase judgment accuracy. To investigate these hypotheses, prospective teachers without prior knowledge in diagnosing misconceptions (N = 81) were asked to diagnose misconceptions on decimal fractions of virtual students by collecting information on students’ solutions. Data concerning the effects of restricting the availability of information on teachers’ cognitive processes were analyzed. The results show that with restricted information, participants indeed select a greater proportion of diagnostically relevant tasks, which positively influences judgment accuracy. These results are discussed with respect to their significance for framing teacher training and for further research.
“Motherhood forced me to cope with my disability”: identity intersection among mothers with physical disabilities
Authors: Shpigelman Carmit-Noa , Karlinski Argi Limor
Published: September 2024

Background

Although motherhood plays a meaningful role in the formation of a woman’s identity, most studies have focused on the process of identity transformation in the transition to motherhood among non-disabled women; less is known about this process among women with physical disabilities who become mothers.

Objective

The present study aimed to understand and describe the subjective experiences of Israeli women with lifelong physical disabilities in their motherhood journey from the perspective of the intersection of their motherhood and disability identities, and from the disability studies approach.

Methods

Semi-structured interviews were conducted with 20 Israeli mothers with visible lifelong physical disabilities who live in the community and raise their children.

Results

Three themes emerged from the interviews: (1) the decision to become a mother: Coping with the disability identity for the first time; (2) The FIRST 3 years: Depending on others as limiting their motherhood identity; (3) after age three: Balancing the motherhood and disability identities.

Conclusion

The transition to motherhood led to identity transformation among women with physical disabilities. Becoming a mother increased the tension between dependence and independence in the context of disability, which also influenced the intersection of their motherhood and disability identities and their wellbeing. Practitioners should provide emotional support to mothers with disabilities and help them embrace the positive aspects of each identity and strike a balance between them.
An action-observation method for studying social perception: a mini-review
Authors: Liu Manlu , Enns James T.
Published: September 2024

An important aspect of any social interaction involves inferring other people’s mental states, intentions, and their likely next actions, by way of facial expression, body posture, eye gaze, and limb movements. An actor’s production of actions during social interactions and the observer’s perception of these actions are thus closely linked. In this review, we outline an action-observation methodology, which not only allows for separate analyses of production and perception, but also promotes the study of the dynamic interaction between these two sides of every social exchange. We review two lines of research that have benefited from its application. The first line focuses on individuals performing tasks alone and the observation of their actions by other individuals in order to make inferences about their attentional states. The second line of study focused on pairs of individuals performing collaborative tasks in naturalistic settings and the observation of these performances by other individuals. We offer several suggestions for how this methodology can be extended to improve on the limitations of the present studies, as well as some suggestions of how to use this methodology to venture into new territory. Our aim is to inspire future research applications of this methodology in order to advance our understanding of social action production and perception.
Fear of progression after cancer recurrence: a mixed methods study
Authors: Stewart Ross James , Humphris Gerald Michael , Donaldson Jayne , Cruickshank Susanne
Published: September 2024

Background

The recurrence of cancer will significantly impact an individual’s quality of life (QoL) as they adjust to living with a condition that is often incurable. Patients remain at risk of further progression following recurrence, but fear of cancer progression (FOP) at this time is not commonly examined. Importantly, these fears are known to reach levels in which there are consequences for QoL.

Methods

This study sought to explore levels of FOP, health-related QoL, anxiety, and depression in patients after a recurrence of their cancer in a longitudinal manner. With the study taking place throughout the COVID-19 pandemic, an assessment of fears related to cancer and the pandemic was included. A sequential mixed method approach was employed for complementarity and expansion purposes. A questionnaire was administered to 44 participants on three different occasions one month apart. A sub-sample of 10 participants then took part in semi-structured interviews.

Findings

FOP was present at moderate levels in patients with a cancer recurrence, with over a third of the sample reaching levels considered dysfunctional. Levels of fear were stable over three months and were not predicted by select demographic or clinical factors. On average, depression was low, but anxiety reached mild levels. Challenges to health-related QoL were evident. Low levels of concern about COVID-19 in relation to cancer were reported. Integrated findings provided more nuanced answers to the research questions, including more specific worries about cancer progression.

Implications

Findings support the development of psychosocial interventions to manage FOP, and future recommendations are provided. Identifying the presence of fears not commonly screened for after cancer recurrence adds to the existing knowledge in this area. Through acknowledging and attending to the psychosocial impact of FOP, healthcare professionals can provide tailored support to enhance the well-being of those with a recurrence of their cancer.
Altered sense of agency in schizophrenia: the aberrant effect of cardiac interoceptive signals
Authors: Koreki Akihiro , Terasawa Yuri , Nuruki Atsuo , Oi Hiroki , Critchley Hugo , Yogarajah Mahinda , Onaya Mitsumoto
Published: September 2024

Background

Schizophrenia (SZ) is characterized by abnormalities in self-representation, including a disturbed sense of agency (SoA). The continuous processing of sensory information concerning the internal state of the body (interoception) is argued to be fundamental to neural representations of the self. We, therefore, tested if aberrant interoception underpins disturbances in SoA in SZ, focusing on cardiac interoceptive signaling.

Methods

Forty-two SZ and 29 non-clinical participants (healthy controls; HC) performed an intentional binding task to measure SoA during concurrent heartbeat recording. The effect of cardiac interoceptive signals on SoA was measured by the difference in intentional binding effect during systole and diastole. This measure was standardized based on the overall intentional binding effect to control for non-cardiac factors, and then compared between SZ and HC.

Results

Our study revealed a significant difference between SZ and HC groups, with opposite effects of cardiac systole on SoA. Specifically, cardiac systole disrupted SoA in SZ, contrasting with the enhanced SoA in HC. Across the SZ group, the extent to which SoA was disrupted by cardiac systole correlated significantly with a clinical proxy for symptom instability, namely the number of hospital admissions for hallucinations and delusions. Furthermore, the disruption was particularly observed in patients with severe hallucinations.

Conclusions

This study revealed a disturbance in the impact of cardiac interoceptive signals on an implicit index of SoA in schizophrenia. This supports the notion that pathophysiological disruption of the central integration of interoceptive information increases vulnerability to disturbances in self-representation and the associated expression of schizophrenic symptoms.
Unveiling the gut microbiota blueprint of schizophrenia: a multilevel omics approach
Authors: Qi DongDong , Liu Peng , Wang YiMeng , Tai XuGuang , Ma ShiFa
Published: September 2024

Background

Schizophrenia is a persistent incurable mental disorder and is characterized by the manifestation of negative emotions and behaviors with anxiety and depression, fear and insecurity, self-harm and social withdrawal. The intricate molecular mechanisms underlying this phenomenon remain largely elusive. Accumulating evidence points towards the gut microbiota exerting an influence on brain function via the gut-brain axis, potentially contributing to the development of schizophrenia. Therefore, the objective of this study is to delineate the gut microbial composition and metabolic profile of fecal samples from individuals with schizophrenia.

Methods

Liquid chromatography-mass spectrometry (LC-MS) and 16S ribosomal RNA (16S rRNA) gene sequencing were employed to analyze fecal metabolites and gut microbiota profiles in a cohort of 29 patients diagnosed with schizophrenia and 30 normal controls. The microbial composition of fecal samples was determined through the 16S rRNA gene sequencing, and microbial α-diversity and β-diversity indices were calculated. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were performed to analyze the distribution of samples. The metabolites and gut microbiota exhibiting differential expression were identified through the application of biological variance criteria. Co-occurrence analysis of bacteria and metabolites was conducted using the spearman’s rank correlation coefficient and visualized in a circular layout with the Cytoscape software.

Results

The findings of the study indicated a lack of substantial evidence supporting significant disparities in α-diversity and β-diversity between individuals with schizophrenia and normal controls. In terms of metabolomics, a discernible pattern in sample distribution between the two groups was observed. Our analysis has revealed 30 bacterial species and 45 fecal metabolites that exhibited notable differences in abundance between individuals diagnosed with schizophrenia and normal controls. These alterations in multilevel omics have led to the development of a co-expression network associated with schizophrenia. The perturbed microbial genes and fecal metabolites consistently demonstrated associations with amino acid and lipid metabolism, which play essential roles in regulating the central nervous system.

Conclusion

Our results offered profound insights into the impact of imbalanced gut microbiota and metabolism on brain function in individuals with schizophrenia.
Prevalence and trends of autism spectrum disorder and other developmental disabilities among children and adolescents in the United States from 2019 to 2021
Authors: Ge Wenrong , Zhang Cancan , Yang Guang , Zhang Bo
Published: September 2024

Background

The National Health Interview Survey (NHIS) is a comprehensive health survey conducted by the National Center for Health Statistics (NCHS) in the U.S., providing valuable insights into the health status of the population. This study focuses on the NHIS child survey between 2019 and 2021, exploring developmental disabilities in U.S. children, including autism spectrum disorder (ASD), attention deficit/hyperactivity disorder or attention-deficit disorder (ADHD/ADD), intellectual disability (ID), other developmental delay (other DD), and learning disability (LD).

Objective

Leveraging NHIS data, our primary objective is to investigate the latest trends and disparities in the prevalence of developmental disabilities among various racial-ethnic groups.

Methods

Employing a repeated cross-sectional design, we analyzed NHIS data from 2019 to 2021, focusing on children aged 3-17. The survey employed a meticulous stratified multi-stage sampling design. We utilized SAS version 9.4 for data analysis, calculating race-ethnicity-specific prevalence rates and employing weighted linear regression and the Rao-Scott chi-square test for trend analysis.

Results

Among 19,490 children, prevalence rates varied: ASD (3.11%), ADHD/ADD (9.50%), ID (1.85%), other DD (5.66%), and LD (7.49%). Non-Hispanic black children exhibited higher rates of ID and LD, while non-Hispanic white children had the highest ADHD/ADD prevalence. Disparities persisted across sociodemographic subgroups, with variations in prevalence rates.

Conclusion

Our study reveals an increase in ASD prevalence and persistent disparities among racial-ethnic groups. Non-Hispanic black children face elevated risks of ID and LD, while non-Hispanic white children exhibit higher rates of ADHD/ADD.
Can technology be good for health? Investigating health-promoting strategies in the private sector
Authors: Sigler Brittany E. , Pollack Porter Keshia M. , Thompson Lindsay , Singer Sara , Gaskin Darrell J.
Published: September 2024

Introduction

This research investigates what might motivate tech companies and impact-driven investors to adopt a health-promoting strategy in their product development and capital allocation strategies.

Methods

Participants were recruited for semi-structured interviews through purposive and snowball sampling. From 83 outreach attempts, thematic saturation required 19 completed interviews out of the 46 consumer technology executives and impact-focused investors who responded. Interviews were analyzed using grounded theory-based content analysis.

Results

Seven coding categories resulted from inductive coding, with 83 sub-codes. The primary themes were: product-based health impact is magnified when matched to user demographics (making an equity mindset important); stakeholders are eager for reliable health metrics, especially those that hold across industry verticals; when capturing health impact, it is critical to include positive (i.e., economically beneficial) externalities. These results allowed for the creation of a logic model with a recommended theory of change for the private sector to develop health strategy.

Discussion

Intentional integration of impact strategy with business priorities will allow teams to design products that promote health, driving buy-in and resource allocation while attracting investment and double returns. For policymakers, it is clear that tech policy and regulation for corporate reporting need to keep pace. These findings are limited by the purposive recruitment of participants, introducing potential bias and risk to generalizability.
Generative artificial intelligence in the agri-food value chain - overview, potential, and research challenges
Authors: Krupitzer Christian
Published: September 2024

ChatGPT uses a so called Large Language Model (LLM) to provide textual output of analyzed data. Those LLMs are one example for Generative Artificial Intelligence (AI), which focuses on creating new content, e.g., text, images, or music, based on learned patterns. Recently, applications in the food industry and agriculture started to apply Generative AI. This mini review provides an overview about applications of Generative AI in the agri-food supply chain and discusses open research challenges, also in combination with digital twins.
Effects of switching from twice-daily tacrolimus to once-daily extended-release meltdose tacrolimus on cellular immune response
Authors: Anft Moritz , Zgoura Panagiota , Skrzypczyk Sarah , Dürr Michael , Viebahn Richard , Westhoff Timm H. , Stervbo Ulrik , Babel Nina
Published: September 2024

Background

LCP-Tacro [LCPT], a novel once-daily, extended-release formulation of tacrolimus, has a reduced Cmax with comparable AUC exposure, requiring a ∼30% dose reduction in contrast to immediate-release tacrolimus (IR-Tac). Once-daily LCPT in de novo kidney transplantation has a comparable efficacy and safety profile to that of IR-Tac with advantages in bioavailability and absorption. The present investigation intends to analyze the effects of conversion from IR-Tac to LCPT on phenotype and function of T-cells and B-cells.

Methods

16 kidney transplant patients treated by triple standard immunosuppression with a stable graft function undergoing a switch from IR-Tac to LCPT were included in this observational prospective study. We measured the main immune cell types and performed an in-depth characterization of B cell, dendritic cells and T cells including regulatory T cells of the patients before, 4 and 8 weeks after IR-Tac to LCPT conversion using multi-parameter fl ow cytometry. Additionally, we analyzed T cells by assessing third-party antigens (Tetanus Diphtheria, TD)-reactive T cells, which could be analyzed by restimulation with tetanus vaccine.

Results

Overall, we found no significant alterations following LCPT conversion for the most immune cell populations with a few cell populations showing transient quantitative increase. Thus, 4 weeks after conversion, more regulatory T cells could be measured in the patients with a significant shift from memory to naïve Tregs. Furthermore, we found a transient B cell expansion 4 weeks after conversion from IR-Tac to LCPT. There were no changes in the percentage of other basic immune cell types and the antigen-reactive T cells were also not altered after changing the medication to LCP-tacrolimus.

Conclusion

Here, we demonstrate first insights into the immune system changes occurred under IR-Tac to LCPT conversion therapy in kidney transplant patients. While phenotypic and functional characteristics of the most immune cell populations did not change, we could observe an a transient expansion of regulatory T cells in peripheral blood following IR-Tac to LCTP conversion, which might additionally contribute to the overall immunosuppressive effect.
Digital Migration Infrastructure in return-writing: visualizing the migration landscape of India
Authors: Mukherjee Preetha , Menon Nirmala
Published: September 2024

Digitization has carved out the migration patterns of immigrants over the recent years of increased technological interventions in human mobility. Migration infrastructures, which typically refer to the physical, commercial, humanitarian, and governmental modes of operation, are multidimensional in nature. Digital infrastructures are equally important to the physical ones as digital technologies facilitate these migration processes through agents like hardware, software, and mediating actors. Amongst the multiple forms of migration, the concept of return-writing and nostalgia-struck-returnees encompass individuals whose life trajectories run parallel to the homeland. The narrative of return to the homeland emerges aβs a dominant motif in literature due to the rising trends of globalization, the writers’ reflection on their own migrant experiences, and publishing trends meeting the demand of the global book market. The objective is to assess the role of digital migration infrastructures in return migrations to India through a close reading of the selected texts and review of postcolonial literary theories by using conditional operation in Python. The study here explores the varied nuances of return migration with a primary focus on the external conditions of travel in migration literature. The paper aims to analyze the genre of return-writing in Indian English literature, through three novels over a period of two decades, i.e., from 2000–2023. The selected texts, beginning with Amit Chaudhuri’s A New World (2000), Gun Island (2019) by Amitav Ghosh, and Devika Rege’s novel Quarterlife (2023), offer a panoramic view of return migration. These novels are extensive in the time period of technological interventions and in depiction of return migration. The Python code examines the extent of existence of a set of digital migration infrastructure keywords by analyzing the content of the novels and creates bar plots and charts to offer a visual representation of the classification results. The resulting trend traces the increased intervention of Digital Migration Infrastructure in the recent migration literature.
Case report: Diffuse large B-cell lymphoma presenting as congestive heart failure in a cat
Authors: Johnson Jake , Melhorn Hannah , Karchemskiy Sonya , Karlin Emily , Bain Perry , Rush John , Peterson Cornelia
Published: September 2024

Cardiac lymphoma is uncommon in cats and is rarely considered as a differential diagnosis for congestive heart failure. A 10-year-old neutered male domestic short-haired cat with clinical histories of feline immunodeficiency virus, diabetes mellitus, and congestive heart failure was humanely euthanized. Post-mortem evaluation demonstrated a massively infiltrative round cell neoplasm of the heart, resulting in CHF. Immunohistochemistry of neoplastic tissue was consistent with diffuse large B-cell lymphoma. This case demonstrates a peculiar presentation of cardiac diffuse large B-cell lymphoma, with chronic feline lentiviral infection possibly contributing to disease initiation and progression.
Withania somnifera (Ashwagandha) Improves Spatial Memory, Anxiety and Depressive-like Behavior in the 5xFAD Mouse Model of Alzheimer’s Disease
Authors: Gladen-Kolarsky Noah , Monestime Olivia , Bollen Melissa , Choi Jaewoo , Yang Liping , Magaña Armando Alcazar , Maier Claudia S. , Soumyanath Amala , Gray Nora E.
Published: September 2024

Withania somnifera (WS), also known as ashwagandha, is a popular botanical supplement used to treat various conditions including memory loss, anxiety and depression. Previous studies from our group showed an aqueous extract of WS root (WSAq) enhances cognition and alleviates markers for depression in Drosophila. Here, we sought to confirm these effects in the 5xFAD mouse model of β-amyloid (Aβ) accumulation. Six- to seven-month-old male and female 5xFAD mice were treated with WSAq in their drinking water at 0 mg/mL, 0.5 mg/mL or 2.5 mg/mL for four weeks. In the fourth week of treatment, spatial memory, anxiety and depressive-like symptoms were evaluated. At the conclusion of behavioral testing, brain tissue was harvested, immunohistochemistry was performed, and the cortical expression of antioxidant response genes was evaluated. Both concentrations of WSAq improved spatial memory and reduced depressive and anxiety-related behavior. These improvements were accompanied by a reduction in Aβ plaque burden in the hippocampus and cortex and an attenuation of activation of microglia and astrocytes. Antioxidant response genes were upregulated in the cortex of WSAq-treated mice. Oral WSAq treatment could be beneficial as a therapeutic option in AD for improving disease pathology and behavioral symptoms. Future studies focused on dose optimization of WSAq administration and further assessment of the mechanisms by which WSAq elicits its beneficial effects will help inform the clinical potential of this promising botanical therapy.
Metabolomic Profiling Reveals That Exercise Lowers Biomarkers of Cardiac Dysfunction in Rats with Type 2 Diabetes
Authors: Wang Tutu , Ning Miaomiao , Mo Yurou , Tian Xinyu , Fu Yu , Laher Ismail , Li Shunchang
Published: September 2024

The increasing prevalence of type 2 diabetes mellitus (T2DM) leads to significant global health challenges, including cardiac structural and functional deficits, which in severe cases can progress to heart failure that can further strain healthcare resources. Aerobic exercise can ameliorate cardiac dysfunction in individuals with diabetes, although a comprehensive understanding of its underlying mechanisms remains elusive. This study utilizes untargeted metabolomics to reveal aerobic-exercise-activated metabolic biomarkers in the cardiac tissues of Sprague Dawley rats with T2DM. Metabolomics analysis revealed that diabetes altered 1029 myocardial metabolites, while aerobic exercise reversed 208 of these metabolites, of which 112 were upregulated and 96 downregulated. Pathway topology analysis suggested that these metabolites predominantly contributed to purine metabolism and arginine biosynthesis. Furthermore, receiver operating characteristic curve analysis identified 10 potential biomarkers, including xanthine, hypoxanthine, inosine, dGMP, l-glutamic acid, l-arginine, l-tryptophan, (R)-3-hydroxybutyric acid, riboflavin, and glucolepidiin. Finally, data from Pearson correlation analysis indicated that some metabolic biomarkers strongly correlated with cardiac function. Our data suggest that certain metabolic biomarkers play an important role in ameliorating diabetes-related cardiac dysfunction by aerobic exercise.
Association of Serum Malondialdehyde Levels with Lipid Profile and Liver Function in Patients with Inflammatory Bowel Disease
Authors: Merino de Paz Nayra , Carrillo-Palau Marta , Hernández-Camba Alejandro , Abreu-González Pedro , de Vera-González Antonia , González-Delgado Alejandra , Martín-González Candelaria , González-Gay Miguel Á. , Ferraz-Amaro Iván
Published: September 2024

Malondialdehyde (MDA) is a naturally occurring organic compound produced as a byproduct of lipid peroxidation. It serves as one of the most widely recognized biomarkers for oxidative stress. Elevated levels of MDA have been observed in patients with inflammatory bowel disease (IBD), suggesting its involvement in the pathogenesis and progression of the disease. In this study, we analyzed MDA levels within a well-characterized and extensive cohort of IBD patients. Our objective was to investigate the association between MDA levels and disease characteristics in this population. This is a cross-sectional study that encompassed 197 patients with IBD. Multivariable linear regression analysis was performed to study the relationship between disease characteristics and circulating MDA. MDA was significantly associated with male sex in IBD patients but not with other demographic characteristics or classic cardiovascular risk factors. Regarding disease features such as phenotype or activity indices, their relationship with MDA was scarce. Several lipid profile molecules showed a significant association with MDA levels after multivariable analysis. Similarly, the liver fibrosis-4 index and hepatic elastography values were significantly related to higher MDA levels after adjusting for covariates. In conclusion, the sources of elevated MDA in IBD are primarily linked to lipid profile abnormalities and liver disease.
Machine Learning and Optical-Coherence-Tomography-Derived Radiomics Analysis to Predict the Postoperative Anatomical Outcome of Full-Thickness Macular Hole
Authors: Hu Yuqian , Meng Yongan , Liang Youling , Zhang Yiwei , Chen Biying , Qiu Jianing , Meng Zhishang , Luo Jing
Published: September 2024

Full-thickness macular hole (FTMH) leads to central vision loss. It is essential to identify patients with FTMH at high risk of postoperative failure accurately to achieve anatomical closure. This study aimed to construct a predictive model for the anatomical outcome of FTMH after surgery. A retrospective study was performed, analyzing 200 eyes from 197 patients diagnosed with FTMH. Radiomics features were extracted from optical coherence tomography (OCT) images. Logistic regression, support vector machine (SVM), and backpropagation neural network (BPNN) classifiers were trained and evaluated. Decision curve analysis and survival analysis were performed to assess the clinical implications. Sensitivity, specificity, F1 score, and area under the receiver operating characteristic curve (AUC) were calculated to assess the model effectiveness. In the training set, the AUC values were 0.998, 0.988, and 0.995, respectively. In the test set, the AUC values were 0.941, 0.943, and 0.968, respectively. The OCT-omics scores were significantly higher in the “Open” group than in the “Closed” group and were positively correlated with the minimum diameter (MIN) and base diameter (BASE) of FTMH. Therefore, in this study, we developed a model with robust discriminative ability to predict the postoperative anatomical outcome of FTMH.
Characterization of Amnion-Derived Membrane for Clinical Wound Applications
Authors: Ingraldi Alison L. , Allen Tim , Tinghitella Joseph N. , Merritt William C. , Becker Timothy , Tabor Aaron J.
Published: September 2024

Human amniotic membrane (hAM), the innermost placental layer, has unique properties that allow for a multitude of clinical applications. It is a common misconception that birth-derived tissue products, such as dual-layered dehydrated amnion–amnion graft (dHAAM), are similar regardless of the manufacturing steps. A commercial dHAAM product, Axolotl Biologix DualGraft™, was assessed for biological and mechanical characteristics. Testing of dHAAM included antimicrobial, cellular biocompatibility, proteomics analysis, suture strength, and tensile, shear, and compressive modulus testing. Results demonstrated that the membrane can be a scaffold for fibroblast growth (cellular biocompatibility), containing an average total of 7678 unique proteins, 82,296 peptides, and 96,808 peptide ion variants that may be antimicrobial. Suture strength results showed an average pull force of 0.2 N per dHAAM sample (equating to a pull strength of 8.5 MPa). Tensile modulus data revealed variation, with wet samples showing 5× lower stiffness than dry samples. The compressive modulus and shear modulus displayed differences between donors (lots). This study emphasizes the need for standardized processing protocols to ensure consistency across dHAAM products and future research to explore comparative analysis with other amniotic membrane products. These findings provide baseline data supporting the potential of amniotic membranes in clinical applications.
Dynamics of Treatment Response to Faricimab for Diabetic Macular Edema
Authors: Fasler Katrin , Muth Daniel R. , Cozzi Mariano , Kvanta Anders , Rejdak Magdalena , Blaser Frank , Zweifel Sandrine A.
Published: September 2024

This study analyzes the dynamics of short-term treatment response to the first intravitreal faricimab injection in eyes with diabetic macular edema (DME). This retrospective, single-center, clinical trial was conducted at the Department of Ophthalmology, University Hospital Zurich. Patients with treatment-naïve and pretreated DME were included. Patient chart data and imaging were analyzed. Safety and efficacy (corrected visual acuity (CVA), central subfield thickness (CST), and signs of intraocular inflammation (IOI)) of the first faricimab intravitreal therapy (IVT) were evaluated weekly until 4 weeks after injection. Forty-three eyes (81% pretreated) of 31 patients were included. Four weeks after the first faricimab IVT, CVA remained stable and median CST (µm) decreased significantly (p < 0.001) from 325.0 (293.5–399.0) at baseline to 304.0 (286.5–358.0). CVA at week 4 was only associated with baseline CVA (p < 0.001). CST was the only predictive variable (p = 0.002) between baseline and week 4 CST. Weekly safety assessments did not show any sign of clinically significant IOI. This study suggests faricimab is an effective treatment for (pretreated) DME, showing structural benefit 1 month following the first injection without short-term safety signals.