THE ETHYL ACETATE FRACTION OF PSOROSPERMUM FEBRIFUGUM SPACHROOT BARKS AQUEOUS EXTRACT IS A GOOD STIMULATOR OF HEMATOPOIESIS
Authors:   Experimental and Clinic Biology Laboratory, National School of Applied Biosciences and Biotechnology, National University of Science, Technology, Engineering and Mathematics (UNSTIM), Dassa-Zoume, R. Benin., Senou Maximin ,   Research Laboratory in Applied Biology, Polytechnic School ofAbomey-Calavi, University of Abomey-Calavi, Cotonou, R. Benin., Atchade Tchogou Pascal ,   Experimental and Clinic Biology Laboratory, National School of Applied Biosciences and Biotechnology, National University of Science, Technology, Engineering and Mathematics (UNSTIM), Dassa-Zoume, R. Benin.,   Research Laboratory in Applied Biology, Polytechnic School ofAbomey-Calavi, University of Abomey-Calavi, Cotonou, R. Benin., Agbogba Felicienne ,   Experimental and Clinic Biology Laboratory, National School of Applied Biosciences and Biotechnology, National University of Science, Technology, Engineering and Mathematics (UNSTIM), Dassa-Zoume, R. Benin.,   Laboratory of Biomembrane and CellSignaling, Faculty of Sciences and Techniques, University ofAbomey-Calavi, R Benin., Jacques Lokonon Ezeckiel ,   Experimental and Clinic Biology Laboratory, National School of Applied Biosciences and Biotechnology, National University of Science, Technology, Engineering and Mathematics (UNSTIM), Dassa-Zoume, R. Benin., Medoatinsa Esperance ,   Experimental and Clinic Biology Laboratory, National School of Applied Biosciences and Biotechnology, National University of Science, Technology, Engineering and Mathematics (UNSTIM), Dassa-Zoume, R. Benin., Estelle Kanfon Rose ,   Laboratory of Study and Research in Applied Chemistry, Polytechnic School ofAbomey-Calavi, University ofAbomey-Calavi, R. Benin., Tamegnon Dougnon Victorien ,   Research Laboratory in Applied Biology, Polytechnic School ofAbomey-Calavi, University of Abomey-Calavi, Cotonou, R. Benin., Cokou Dossa Agbangnan Pascal ,   Laboratory of Study and Research in Applied Chemistry, Polytechnic School ofAbomey-Calavi, University ofAbomey-Calavi, R. Benin., Boko Cyrille ,   Research Laboratory in Applied Biology, Polytechnic School ofAbomey-Calavi, University of Abomey-Calavi, Cotonou, R. Benin., Loko Frederic ,   Research Laboratory in Applied Biology, Polytechnic School ofAbomey-Calavi, University of Abomey-Calavi, Cotonou, R. Benin., Laleye Anatole ,   Human Biology Unit, Faculty of Health Science, Cotonou, R. Benin., Agbonon Amegnona ,   Laboratoire de Physiologie/Pharmacologie, Faculte des Sciences, Universite de Lome, R. Togo., Saïd Baba-Moussa Lamine ,   Laboratoire de Biologie et de TypageMoleculaire en Microbiologie, University of Abomey-Calavi R. Benin., Sezan Alphonse ,   Laboratory of Biomembrane and CellSignaling, Faculty of Sciences and Techniques, University ofAbomey-Calavi, R Benin.
Published: November 2020

PsorospermumfebrifugumSpach. (Clusiaceae) is a medicinal plant found in Benin whose root bark was effective in treating anemia. To identify the family of chemical compounds of this organ was responsible for its hematopoietic efficiency, this work aimed to test the ethyl acetate fraction of the aqueous extract of the plant organ on anemic rats. Methods: Wistar rats were anemic by phenylhydrazinechloridrate on D0. From day 2 to day 15, some were gavage fed with the ethyl acetate fraction of PsorospermumfebrifugumSpach root bark aqueous extract at 40 or 60 mg / kg / day. Others received either vitafer as a reference drug or distilled water (untreated anemic group). Blood samples were collected from these rats and non-anemic control rats at days 0, 2, 7, 10 and 15 for the blood count and osmotic resistance of red blood cells. Results: At D2, phenylhydrazine significantly decreased hemoglobin and red blood cell number, which were corrected on D7 by the extract fraction with a dose-dependent effect. The extract fraction rapidly stimulated release of macrocytes, immature red blood cells in the first week to compensate the anemia. The extract did not affect blood platelet number, suggesting some specificity of action on the red blood cell line. Conclusion: The ethyl acetate fraction of Psorospermumfebrifugum root bark aqueous extract stimulated erythropoiesis faster than the crude extract. Its action seemed specific and dose-dependent. It would probably be related to the flavonoids which action mechanism needs to be explored.