bluuebunny/crossref_metadata_2025 huggingface dataset:
Safety of titanium dioxide (E171) as a food additive for humans
Authors: Warheit David B.
Published: July 2024

Titanium dioxide (TiO2), also known as E171, is commonly used as a white colorant in food, pharmaceuticals, cosmetics, and toothpaste. However, in May 2021, the European Food Safety Authority (EFSA) expert panel, in evaluating the safety of titanium dioxide (E171) as a food additive, concluded that a concern for genotoxicity could not be ruled out. This occurred several years after EFSA had previously considered titanium dioxide to be safe as a food additive. EFSA based this new interpretation on the results of genotoxicity tests of TiO2 nanomaterials. EFSA noted that available data are insufficient to define threshold doses/concentrations of TiO2 particles below which genotoxicity will not occur in tissues containing these particles. Here, it is argued that EFSA made a manifest error regarding the safety of titanium dioxide (E171) particles as a food additive for humans. First, the notion of particle size distribution of TiO2 particles is explained. Second, the changing opinions from the various EFSA evaluations in 2016, 2018, 2019 vs. 2021 are discussed. Third, the low toxicity of TiO2 particles is described in rats exposed by oral gavage and feeding studies in rats and mice. Fourth, the importance of low absorption rates from the gastrointestinal tract vs. circulation in rats and humans but not in mice is identified. Fifth, other international health scientists have weighed in on the EFSA (EFSA J, 2021, 19 (5), 6585) decision and generally disagreed with EFSA’s opinion on the safety of E171 TiO2. A common theme voiced by the United Kingdom, Canada, Australia, and New Zealand agencies is that it is inappropriate to compare nanoparticle toxicity studies of dispersed/sonicated nanoparticles with the content of E171 TiO2 in foods because the test materials used in key studies considered by EFSA (EFSA J, 2021, 19 (5), 6585) are not representative of E171 TiO2 particles. Finally, a group of experts recently considered the genotoxicity of TiO2 and could not find support for a direct DNA damaging mechanism of TiO2 (nano and other forms). For these reasons, it is suggested that EFSA made a manifest error on the safety of E171 as a food additive.
Postbiotic derived from Bacillus subtilis ACCC 11025 improves growth performance, mortality rate, immunity, and tibia health in broiler chicks
Authors: Li Desheng , Fang Shan , He Feng , Fan Xinyan , Wang Tieliang , Chen Zeliang , Wang Mi
Published: July 2024

Introduction

The objective of this study was to evaluate the effects of dietary supplementation of postbiotics on growth performance, mortality rate, immunity, small intestinal health, tibia characteristics, and hematological parameters of broiler chicks. he postbiotics were derived from Bacillus subtilis ACCC 11025.

Methods

A total of 480 day-old Arbor acre broiler chicks (52.83 ± 1.38 g) were used in a 42-day study and were randomly allocated into four groups. Each group comprised 6 replicate cages, each containing 20 birds. Dietary treatments were based on a basal diet, supplemented with postbiotics at concentrations of 0.000%, 0.015%, 0.030%, or 0.045%.

Results and discussion

The results demonstrated an improvement in growth performance, antibody titers against avian influenza virus and Newcastle disease virus, serum albumin levels, and serum total protein levels, as well as a reduction in mortality rate among broiler chicks with increasing levels of postbiotic supplementation. The most significant effect were observed in the group receiving 0.015% postbiotics. Furthermore, a dose-dependent enhancement in tibia weight and tibia weight to length ratio, coupled with a reduction in the robusticity index, was noted. The most favorable outcomes for tibia health were observed in the group receiving 0.030% postbiotics. This improvement in tibia health corresponded to a linear increase in serum calcium and inorganic phosphorus contents. In summary, supplementing broiler chicks with 0.015% postbiotics effectively enhances immunity, leading to improved growth performance and reduced mortality rates. Additionally, a postbiotic dose of 0.030% is suitable for optimizing tibia health.
Rescuing Newcastle disease virus with tag for screening viral-host interacting proteins based on highly efficient reverse genetics
Authors: Wang Ruiwei , Cao Xuhong , Lu Kejia , Chang Zhengwu , Dong Xiaoyu , Guo Hanwei , Wei Xi , Dang Ruyi , Wang Juan , Wang Xinglong , Xiao Sa , Liu Haijin , Yang Zengqi
Published: July 2024

The interaction between viral proteins and host proteins plays a crucial role in the process of virus infecting cells. Tags such as HA, His, and Flag do not interfere with the function of fusion proteins and are commonly used to study protein–protein interactions. Adding these tags to viral proteins will address the challenge of the lack of antibodies for screening host proteins that interact with viral proteins during infection. Obtaining viruses with tagged fusion proteins is crucial. This study established a new reverse genetic system with T7 promoter and three plasmids, which efficiently rescued Newcastle disease virus (NDV) regardless of its ability to replicate in cells. Subsequently, using this system, NDV containing a HA-tagged structural protein and NDV carrying a unique tag on each structural protein were successfully rescued. These tagged viruses replicated normally and exhibited genetic stability. Based on tag antibodies, every NDV structural protein was readily detected and showed correct subcellular localization in infected cells. After infecting cells with NDV carrying HA-tagged M protein, several proteins interacting with the M protein during the infection process were screened using HA tag antibodies. The establishment of this system laid the foundation for comprehensive exploration of the interaction between NDV proteins and host proteins.
Evaluation of candidate data-based welfare indicators for veal calves in Switzerland
Authors: Zwygart Sibylle , Lutz Barbara , Thomann Beat , Stucki Dimitri , Meylan Mireille , Becker Jens
Published: July 2024

Welfare assessment protocols have been developed for dairy cows and veal calves during the past decades. One practical use of such protocols may be conducting welfare assessments by using routinely collected digital data (i.e., data-based assessment). This approach can allow for continuous monitoring of animal welfare in a large number of farms. It recognises changes in the animal welfare status over time and enables comparison between farms. Since no comprehensive data-based assessment for veal calves is currently available, the purposes of this review are (i) to provide an overview of single existing data-based indicators for veal calves and (ii) to work out the necessary requirements for data-based indicators to be used in a comprehensive welfare assessment for veal calves in Switzerland. We used the Welfare Quality Protocol® (WQ) for veal calves and the Terrestrial Animal Health Code from the World Organisation of Animal Health for guidance throughout this process. Subsequently, routinely collected data were evaluated as data sources for welfare assessment in Swiss veal operations. The four WQ principles reflecting animal welfare, i.e., ‘good feeding’, ‘good housing’, ‘good health’ and ‘appropriate behaviour’ were scarcely reflected in routinely available data. Animal health, as one element of animal welfare, could be partially assessed using data-based indicators through evaluation of mortality, treatments, and carcass traits. No data-based indicators reflecting feeding, housing and animal behaviour were available. Thus, it is not possible to assess welfare in its multidimensionality using routinely collected digital data in Swiss veal calves to date. A major underlying difficulty is to differentiate between veal calves and other youngstock using routine data, since an identifying category for veal calves is missing in official Swiss databases. In order to infer animal welfare from routine data, adaptations of data collection strategies and animal identification are required. Data-based welfare assessment could then be used to complement on-farm assessments efficiently and, e.g., to attribute financial incentives for specifically high welfare standards accordingly.
A Rational Approximation of the Two-Term Machin-like Formula for π
Authors: Abrarov Sanjar M. , Siddiqui Rehan , Jagpal Rajinder Kumar , Quine Brendan M.
Published: July 2024

In this work, we consider the properties of the two-term Machin-like formula and develop an algorithm for computing digits of π by using its rational approximation. In this approximation, both terms are constructed by using a representation of 1/π in the binary form. This approach provides the squared convergence in computing digits of π without any trigonometric functions and surd numbers. The Mathematica codes showing some examples are presented.
A Comprehensive Review of Surface Ozone Variations in Several Indian Hotspots
Authors: Keerthi Lakshmi K. A. , Nishanth T. , Satheesh Kumar M. K. , Valsaraj K. T.
Published: July 2024

Ozone at ground level (O3) is an air pollutant that is formed from primary precursor gases like nitrogen oxides (NOx) and volatile organic compounds (VOCs). It plays a significant role as a precursor to highly reactive hydroxyl (OH) radicals, which ultimately influence the lifespan of various gases in the atmosphere. The elevated surface O3 levels resulting from anthropogenic activities have detrimental effects on both human health and agricultural productivity. This paper provides a comprehensive analysis of the variations in surface O3 levels across various regions in the Indian subcontinent, focusing on both spatial and temporal changes. The study is based on an in-depth review of literature spanning the last thirty years in India. Based on the findings of the latest study, the spatial distribution of surface O3 indicates a rise of approximately 50–70 ppbv during the summer and pre-monsoon periods in the northern region and Indo-Gangetic Plain. Moreover, elevated levels of surface O3 (40–70 ppbv) are observed during the pre-monsoon/summer season in the western, southern, and peninsular Indian regions. The investigation also underscores the ground-based observations of diurnal and seasonal alterations in surface O3 levels at two separate sites (rural and urban) in Kannur district, located in southern India, over a duration of nine years starting from January 2016. The O3 concentration exhibits an increasing trend of 7.91% (rural site) and 5.41% (urban site), ascribed to the rise in vehicular and industrial operations. This review also presents a succinct summary of O3 fluctuations during solar eclipses and nocturnal firework displays in the subcontinent.
Three-Dimensional Lightning Characteristics Analysis over the Tibetan Plateau Based on Satellite-Based and Ground-Based Multi-Source Data
Authors: Zhu Jie , Zhi Shulin , Zheng Dong , Yuan Zhengguo
Published: July 2024

Based on the data from the Chinese national ground-based (LFEDA: Low-frequency E-field Detection Array) and satellite-based lightning-detection systems (LMI: Lightning Mapping Imager), the spatial and temporal distribution statistical properties of all types of lightning over the Tibetan Plateau in the summer of 2022 and 2023 are analyzed, and were compared with those in Hainan, which are under quite different geographical conditions. The discrepancy between ground-based and space-borne lightning detection was also discussed. The main results show the following: (1) the characteristics of lightning activities over the Tibetan Plateau based on multi-source data: Most of the high-value lightning areas were located in the transition zone between lower and higher terrain; the diurnal variation of lightning activity was significant, and the most active period concentrated around 15:00 LST (Local Standard Time, the same below). In addition, lightning activities were significantly increased at 21:00 and 0:00, which was related to the unique topography and night rain phenomenon of the plateau. In terms of lightning types, the number of IC (Intra-Cloud) lightning was more than that of CG (Cloud-to-Ground). The study of IC changes is of great significance to the early warning of the plateau DCSs. The spatial distribution of IC at different altitudes was quite different. (2) Comparison of lightning activities between the Tibetan Plateau and Hainan: The hourly variation of lightning activities in Nagqu showed a single peak, while that in Hainan was characterized by a primary peak and a secondary peak, affected by the enhancement of the boundary stream in the low latitude and altitude area of China. At the peak of convection, the lightning activities in Nagqu were less than 1/3 of that in Hainan. However, the duration of high-frequency lightning activities in Nagqu (15–19:00) was about 2 h longer than that in Hainan (15–17:00), which may be related to the fact that the Tibetan Plateau is located in the west of China, where the sunset is later, and solar radiation and convective activities last longer. (3) Analysis of features of LMI: LMI has more advantages in IC detection; LMI has higher detection efficiency for the lightning in the range of 4–6 KM altitude, which is partly related to the stronger convective process and the higher proportion of IC. This work will provide deeper understanding of the characteristics of all types of lightning over the Tibetan Plateau, to reveal the indication significance of lightning for DCSs, and help to promote the development of Chinese satellite-based lightning-detection technology, the optimization of subsequent instruments and the fusion application of ground-based and satellite-based lightning data.
Hyperacusis in Tinnitus Individuals Is Associated with Smaller Gray Matter Volumes in the Supplementary Motor Area Regardless of Hearing Levels
Authors: Makani Punitkumar , Thioux Marc , Koops Elouise A. , Pyott Sonja J. , van Dijk Pim
Published: July 2024

Recent evidence suggests a connection between hyperacusis and the motor system of the brain. For instance, our recent study reported that hyperacusis in participants with tinnitus and hearing loss is associated with smaller gray matter volumes in the supplementary motor area (SMA). Given that hearing loss can affect gray matter changes in tinnitus, this study aimed to determine if the changes reported in our previous findings of smaller SMA gray matter volumes in hyperacusis persist in the absence of hearing loss. Data for this study were gathered from four prior studies conducted between 2004 and 2019 at the University Medical Centre Groningen (UMCG). A total of 101 participants with tinnitus and either clinically normal hearing (normal hearing with tinnitus or NHT, n = 35) or bilateral sensorineural hearing loss (hearing loss with tinnitus or HLT, n = 66) were included across four studies. Hyperacusis was determined by a score of ≥22 on the Hyperacusis Questionnaire (HQ). In the NHT group, 22 (63%) participants scored ≥22 on the HQ (NHT with hyperacusis: mean age 44.1 years, 12 females), while in the HLT group, 25 (38%) participants scored ≥22 on the HQ (HLT with hyperacusis: mean age 59.5 years, 10 females). The 2 × 2 between-group ANOVAs revealed that hyperacusis is associated with smaller SMA gray matter volumes, regardless of hearing levels. Notably, the smaller SMA gray matter volumes in hyperacusis were primarily influenced by the attentional subscales of the HQ. The association between hyperacusis and the motor system may indicate a constant alertness to sounds and a readiness for motor action.
Brain Magnetic Resonance Imaging in Wilson’s Disease—Significance and Practical Aspects—A Narrative Review
Authors: Litwin Tomasz , Rędzia-Ogrodnik Barbara , Antos Agnieszka , Przybyłkowski Adam , Członkowska Anna , Bembenek Jan Paweł
Published: July 2024

Wilson’s disease (WD) is a genetic disorder of copper metabolism with pathological copper accumulation in many organs, resulting in clinical symptoms, mostly hepatic and neuropsychiatric. As copper accumulates in the brain during WD, and almost 50% of WD patients at diagnosis present with neurological symptoms, neuroimaging studies (especially brain magnetic resonance imaging (MRI)) are part of WD diagnosis. The classical sequences (T1, T2, and fluid-attenuated inversion recovery) were used to describe brain MRI; however, with the development of neuroradiology, several papers proposed the use of new MRI sequences and techniques like susceptibility-weighted images, T2*, diffusion MRI, tractography, volumetric assessment and post-processing brain MRI analysis of paramagnetic accumulation—quantitative susceptibility mapping. Based on these neuroradiological data in WD, currently, brain MRI semiquantitative scale and the pathognomonic neuroradiological brain MRI signs in WD were proposed. Further, the volumetric studies and brain iron accumulation MRI analysis suggested brain atrophy and iron accumulation as biomarkers of neurological WD disease severity. All these results highlight the significance of brain MRI examinations in WD. Due to the extreme progress of these studies, based on the available literature, the authors present the current state of knowledge about the significance, practical aspects, and future directions of brain MRI in WD.
Study on Stability and Deformation Characteristics of Ultra-Deep Diaphragm Wall during Trench Excavation
Authors: Ma Jianhua , Hu Gang , Rui Rui , Ding Ruiheng , Sun Tianjian
Published: July 2024

The stability and deformation during trenching of ultra-deep diaphragm walls have a greater impact on the construction of diaphragm walls. The conventional limit equilibrium method, known as the vertical slices method, assumes homogeneity within the rock and soil mass, making it challenging to account for their stratification. Based on the limit equilibrium method, the horizontal strip method takes into account the stratification of the foundation soil. Based on the actual project, considering the different mud unit weights and heights, the horizontal strip method was used to analyze the stability of the groove trench and analyze the deformation law of the groove trench by the finite element method. The results indicate the following: The horizontal strip method can effectively assess the influence law of mud weight and height on the safety coefficient of groove trench stabilization. The higher the unit weight and level of slurry, the greater the wall safety coefficient. Moreover, the mud unit weight obtained by the horizontal strip method is about 12.70~12.64 kN/m3, which is close to the actual maximum mud weight of 12.5 kN/m3. The estimated mud unit weight aligns more closely with actual conditions. Additionally, through finite element analysis, the deformation law of the groove trench of ultra-deep diaphragm walls has been analyzed and summarized. The soil around the groove trench does not move inward, which shows three different deformation modes with different depths.
Correction: Krayem et al. Kinome Profiling to Predict Sensitivity to MAPK Inhibition in Melanoma and to Provide New Insights into Intrinsic and Acquired Mechanism of Resistance. Cancers 2020, 12, 512
Authors: Krayem Mohammad , Aftimos Philippe , Najem Ahmad , van den Hooven Tim , van den Berg Adriënne , Hovestad-Bijl Liesbeth , de Wijn Rik , Hilhorst Riet , Ruijtenbeek Rob , Sabbah Malak , Kerger Joseph , Awada Ahmad , Journe Fabrice , Ghanem Ghanem E.
Published: July 2024

In the original article [...]
Stem Cell Mobilization Performed with Different Doses of Cytarabine in Plasma Cell Myeloma Patients Relapsing after Previous Autologous Hematopoietic Cell Transplantation—A Multicenter Report by the Polish Myeloma Study Group
Authors: Drozd-Sokołowska Joanna , Waszczuk-Gajda Anna , Topczewska Magdalena , Maciejewska Martyna , Dutka Magdalena , Zaucha Jan Maciej , Szmigielska-Kapłon Anna , Nowicki Mateusz , Olszewska-Szopa Magdalena , Szeremet Agnieszka , Czyż Anna , Kozioł Magdalena , Hus Marek , Mańko Joanna , Hus Iwona , Romejko-Jarosińska Joanna , Kopińska Anna , Helbig Grzegorz , Mądry Krzysztof , Boguradzki Piotr , Król Małgorzata , Snarski Emilian , Hayden Patrick J. , Jamroziak Krzysztof , Dwilewicz-Trojaczek Jadwiga , Basak Grzegorz Władysław
Published: July 2024

Salvage autologous hematopoietic cell transplantation (auto-HCT) may be used to treat relapse of plasma cell myeloma occurring after previous auto-HCT. When an insufficient number of hematopoietic stem cells have been stored from the initial harvest, remobilization is necessary. Here, we aimed to analyze the efficacy and safety of different doses of cytarabine (total 800 vs. 1600 vs. 2400 mg/m2) for remobilization. Sixty-five patients, 55% male, with a median age at remobilization 63 years, were included. Remobilization was performed with cytarabine_800 in 7, cytarabine_1600 in 36, and cytarabine_2400 in 22 patients. Plerixafor rescue was used in 25% of patients receiving cytarabine_1600 and 27% of those receiving cytarabine_2400. Patients administered cytarabine_800 were not rescued with plerixafor. Remobilization was successful in 80% of patients (57% cytarabine_800; 86% cytarabine_1600; 77% cytarabine_2400; p = 0.199). The yield of collected CD34+ cells did not differ between the different cytarabine doses (p = 0.495). Patients receiving cytarabine_2400 were at the highest risk of developing severe cytopenias, requiring blood product support, or having blood-stream infections. One patient died of septic shock after cytarabine_2400. In summary, remobilization with cytarabine is feasible in most patients. All doses of cytarabine allow for successful remobilization. Cytarabine_2400 is associated with higher toxicity; therefore, lower doses (800 or 1600 mg/m2) seem to be preferable.
Characterization of a Syngeneic Orthotopic Model of Cholangiocarcinoma by [18F]FDG-PET/MRI
Authors: Zachhuber Lena , Filip Thomas , Mozayani Behrang , Löbsch Mathilde , Scheiner Stefan , Vician Petra , Stanek Johann , Hacker Marcus , Helbich Thomas H. , Wanek Thomas , Berger Walter , Kuntner Claudia
Published: July 2024

Cholangiocarcinoma (CCA) is a type of primary liver cancer originating from the biliary tract epithelium, characterized by limited treatment options for advanced cases and low survival rates. This study aimed to establish an orthotopic mouse model for CCA and monitor tumor growth using PET/MR imaging. Murine CCA cells were implanted into the liver lobe of male C57BL/6J mice. The imaging groups included contrast-enhanced (CE) MR, CE-MR with static [18F]FDG-PET, and dynamic [18F]FDG-PET. Tumor volume and FDG uptake were measured weekly over four weeks. Early tumor formation was visible in CE-MR images, with a gradual increase in volume over time. Dynamic FDG-PET revealed an increase in the metabolic glucose rate (MRGlu) over time. Blood analysis showed pathological changes in liver-related parameters. Lung metastases were observed in nearly all animals after four weeks. The study concludes that PET-MR imaging effectively monitors tumor progression in the CCA mouse model, providing insights into CCA development and potential treatment strategies.
KRAS Mutation Subtypes and Their Association with Other Driver Mutations in Oncogenic Pathways
Authors: Mondal Koushik , Posa Mahesh Kumar , Shenoy Revathi P. , Roychoudhury Susanta
Published: July 2024

The KRAS mutation stands out as one of the most influential oncogenic mutations, which directly regulates the hallmark features of cancer and interacts with other cancer-causing driver mutations. However, there remains a lack of precise information on their cooccurrence with mutated variants of KRAS and any correlations between KRAS and other driver mutations. To enquire about this issue, we delved into cBioPortal, TCGA, UALCAN, and Uniport studies. We aimed to unravel the complexity of KRAS and its relationships with other driver mutations. We noticed that G12D and G12V are the prevalent mutated variants of KRAS and coexist with the TP53 mutation in PAAD and CRAD, while G12C and G12V coexist with LUAD. We also noticed similar observations in the case of PIK3CA and APC mutations in CRAD. At the transcript level, a positive correlation exists between KRAS and PIK3CA and between APC and KRAS in CRAD. The existence of the co-mutation of KRAS and other driver mutations could influence the signaling pathway in the neoplastic transformation. Moreover, it has immense prognostic and predictive implications, which could help in better therapeutic management to treat cancer.
Photo-Induced Degradation of Priority Air Pollutants on TiO2-Based Coatings in Indoor and Outdoor Environments—A Mechanistic View of the Processes at the Air/Catalyst Interface
Authors: Kotzias Dimitrios
Published: July 2024

In recent decades, numerous studies have indicated the substantial role semiconductors could play in photocatalytic processes for environmental applications. Materials that contain a semiconductor as a photocatalyst have a semi-permanent capacity for removing harmful gases from the ambient air. In this paper, the focus is on TiO2. Heterogeneous photocatalysis using TiO2 leads to the degradation of NO/NO2, benzene, toluene, and other priority air pollutants once in contact with the semiconductor surface. Preliminary evidence indicates that TiO2-containing construction materials and paints efficiently destroy the ozone precursors NO and NO2 by up to 80% and 30%, respectively. Therefore, the development of innovative coatings containing TiO2 as a photocatalyst was in the foreground of research activities. The aim of this was for coatings to be used as building and construction materials, mainly outdoors, e.g., on building façades on high-traffic roads for the degradation of priority air pollutants (NOx and volatile organic compounds) in the polluted urban atmosphere. Though there are advantages connected with the application of TiO2, due to its band gap of 3.2 eV, these are limited. TiO2 is effective only in the UV region (ca. 5%) of the solar spectrum with wavelengths λ < 380 nm. Hence, efforts are made here, as in many research studies, to dope TiO2 with transition metals to increase its activity using visible light, which will extend its application to indoor environments. In our studies, experiments were conducted with 0.1% (w/w) and 1% (w/w) Mn-TiO2 admixtures, and the ability of the modified photocatalysts to degrade NO by both solar and indoor illumination was evaluated. The surface chemistry at the air/catalyst interface, governed by the photoelectric characteristics of TiO2 and the formation of reactive oxygen species with co-occurring redox reactions, is reviewed in this paper. The factors affecting the application of TiO2 for the degradation of priority air pollutants as single compounds or mixtures are discussed. We investigated, particularly, the degradation of mixtures of priority compounds at typical concentrations in ambient air and confined spaces. This is a realistic approach, because pollutants are present as mixtures, rather than as individual compounds in ambient and indoor air. Moreover, organic polymers as paint constituents were found to be the primary source for carbonyl formation, e.g., formaldehyde, acetaldehyde, etc., during the heterogeneous photocatalytic processes conducted on TiO2-enriched coatings.
Solvent-Dependent Triboelectric Output Performance of Poly(vinylidene fluoride–trifluoroethylene–chlorofluoroethylene) Terpolymer
Authors: Hu Ying Chieh , Ahn Hyun Soo , Lee Joo Hyeong , Kim Kyung Hoon , Kim Jong Hun , Jung Jong Hoon
Published: July 2024

The poly (vinylidene fluoride–trifluoroethylene–chlorofluoroethylene) P(VDF-TrFE-CFE) terpolymer has been identified as a promising candidate for the effective conversion of low-frequency mechanical vibrations into electricity. In this study, we provide a comprehensive and systematic investigation of the solvent-dependent mechanical, microstructural, electrical, frictional properties and triboelectric output performance of a relaxor ferroelectric P(VDF-TrFE-CFE) terpolymer. The P(VDF-TrFE-CFE) terpolymer films obtained from high dipole moment solvents have a longer rod-shaped grain than those from low dipole moment solvents. The crystallinity, Young’s modulus and dielectric constant of P(VDF-TrFE-CFE) terpolymer become larger as the dipole moment of solvents increases, while the remnant polarization remains almost the same. The P(VDF-TrFE-CFE) terpolymer film obtained from the highest dipole moment solvent generates almost 1.55 times larger triboelectric charge than that obtained from the lowest moment. We attributed this large difference to the greatly enhanced lateral friction of terpolymer film obtained from high dipole moment solvents.
Characteristics and Outcome of Surgically Treated Patients with Intradural Extra- and Intramedullary Spinal Metastasis—A Single-Center Retrospective Case Series and Review
Authors: Salvotti Hanna Veronika , Lein Alexander , Proescholdt Martin , Schmidt Nils-Ole , Siller Sebastian
Published: July 2024

Objective: Intradural spinal metastases are considered rare. At present, limited information is available on incidence, surgical management, and outcomes. Methods: We conducted a retrospective patient chart review from 2002 to 2024, identifying all patients surgically treated for intradural spinal metastases. Clinical, surgical and survival data were collected and compared to literature data for patients surgically treated for extradural spinal metastases. Results: A total of 172 patients with spinal metastases were identified with 13 patients meeting inclusion criteria (7.6%). The mean age at diagnosis of intradural spinal metastases was 52 ± 22 years, with diverse primaries including lung (n = 3), breast (n = 2), sarcoma (n = 2), and six unique entities. Intradural spinal metastasis was diagnosed on average of 3.3 years after primary diagnosis. In total, we observed five (38%) intradural-extramedullary and eight (62%) intramedullary metastases, located in the cervical (38.5%), thoracic (46.1%) and lumbar spine (15.4%). The most common preoperative symptoms were pain, sensory changes, and gait ataxia (each 76.9%). Gross total resection was achieved in 54%, and local tumor control in 85%. Postoperatively, 92% exhibited clinical improvement or stability. Most frequent adjuvant treatment was radio- and/or chemotherapy in 85%. The average survival after operation for spinal intradural metastases was 5 months, ranging from 1 month to 120 months. The location of the intradural metastasis in the cervical spine was associated with a significantly more favorable survival outcome (compared to thoracic/lumbar location, p = 0.02). Conclusions: Intradural location of spinal metastases is rare (7.6%). Even so, surgical resection is safe and effective for neurological improvement, and survival appears lower compared to the reported survival of extradural spinal metastases.
Explainable AI for Interpretation of Ovarian Tumor Classification Using Enhanced ResNet50
Authors: Guha Srirupa , Kodipalli Ashwini , Fernandes Steven L. , Dasar Santosh
Published: July 2024

Deep learning architectures like ResNet and Inception have produced accurate predictions for classifying benign and malignant tumors in the healthcare domain. This enables healthcare institutions to make data-driven decisions and potentially enable early detection of malignancy by employing computer-vision-based deep learning algorithms. These CNN algorithms, in addition to requiring huge amounts of data, can identify higher- and lower-level features that are significant while classifying tumors into benign or malignant. However, the existing literature is limited in terms of the explainability of the resultant classification, and identifying the exact features that are of importance, which is essential in the decision-making process for healthcare practitioners. Thus, the motivation of this work is to implement a custom classifier on the ovarian tumor dataset, which exhibits high classification performance and subsequently interpret the classification results qualitatively, using various Explainable AI methods, to identify which pixels or regions of interest are given highest importance by the model for classification. The dataset comprises CT scanned images of ovarian tumors taken from to the axial, saggital and coronal planes. State-of-the-art architectures, including a modified ResNet50 derived from the standard pre-trained ResNet50, are implemented in the paper. When compared to the existing state-of-the-art techniques, the proposed modified ResNet50 exhibited a classification accuracy of 97.5 % on the test dataset without increasing the the complexity of the architecture. The results then were carried for interpretation using several explainable AI techniques. The results show that the shape and localized nature of the tumors play important roles for qualitatively determining the ability of the tumor to metastasize and thereafter to be classified as benign or malignant.
Advancements and Policy Implications of Green Hydrogen Production from Renewable Sources
Authors: Wang Leiming , Liu Wei , Sun Haipeng , Yang Li , Huang Liang
Published: July 2024

With the increasingly severe climate change situation and the trend of green energy transformation, the development and utilization of hydrogen energy has attracted extensive attention from government, industry, and academia in the past few decades. Renewable energy electrolysis stands out as one of the most promising hydrogen production routes, enabling the storage of intermittent renewable energy power generation and supplying green fuel to various sectors. This article reviews the evolution and development of green hydrogen policies in the United States, the European Union, Japan, and China, and then summarizes the key technological progress of renewable energy electrolysis while introducing the progress of hydrogen production from wind and photovoltaic power generation. Furthermore, the environmental, social, and economic benefits of different hydrogen production routes are analyzed and compared. Finally, it provides a prospective analysis of the potential impact of renewable energy electrolysis on the global energy landscape and outlines key areas for future research and development.
Occurrence of Central Nervous System Complications of Respiratory Syncytial Virus Infections: A Systematic Review with Meta-Analysis
Authors: Riccò Matteo , Cascio Antonio , Corrado Silvia , Bottazzoli Marco , Marchesi Federico , Gili Renata , Giuri Pasquale Gianluca , Gori Davide , Manzoni Paolo
Published: July 2024

An increasing base of evidence suggests that respiratory syncytial virus (RSV) infections may be associated with neurological complications. In accord with the PRISMA statement, we performed a systematic review and meta-analysis on the occurrence of encephalitis and encephalopathy associated with documented RSV infections. PubMed, Embase, and Scopus databases were searched for eligible observational studies published up to 10 April 2024. Raw data included the occurrence of RSV infections among cases of encephalitis and/or encephalopathy and cases of encephalitis and/or encephalopathy among series of RSV infections. Data were pooled in a random effects model. Case reports were also collected, and their data pooled as a cumulative series. Heterogeneity was assessed using the I2 measure, while reporting bias was assessed by means of funnel plots and regression analysis. A total of 15 studies for a total of 7719 RSV infections and 1631 cases of encephalitis were analyzed. Moreover, 27 case reports and case series were retrieved, for a total of 84 individual cases of encephalitis/encephalopathy occurring during a documented RSV infection. A pooled prevalence of 2.20 cases of encephalitis/encephalopathy per 100 RSV cases (I2 = 99%) was calculated, while a prevalence of RSV infections among cases of encephalitis/encephalopathy was estimated to 3.53 per 100 cases for studies on respiratory specimens (I2 = 48%) and 0.37 per cases on central nervous system (CNS) specimens (I2 = 0%). Detection of RSV within the CNS was relatively rare (17.86% of pooled case reports), being associated with male gender (adjusted odds ratio [aOR] 5.021, 95% confidence interval [95%CI] 1.104 to 22.831) and recovery with long-term sequelae (aOR 5.699, 95%CI 1.152; 28.183). Case fatality ratio was estimated to be 0.43 per 100 cases on observational studies and 10.71% in case reports, a difference likely due to publication bias. In summary, RSV represented a not frequent but notable cause of encephalitis/encephalopathy in adults and children. The paucity of available studies not only recommends a cautious appraisal of our results but stresses the clinical significance of future studies on incident cases of encephalitis and/or encephalopathy.
Analysis of Driving Factors for Vegetation Ecological Quality Based on Bayesian Network
Authors: Cai Jin , Wei Xiaojian , Zhang Fuqing , Xia Yuanping
Published: July 2024

Vegetation is a crucial component of ecosystems, and understanding the drivers and spatial optimization patterns of its ecological quality is vital for environmental management in the middle reaches of the Yangtze River Urban Agglomeration. Traditional evaluations employing single indices may not fully capture the complexity of vegetation elements and require evaluation through various indicators. Therefore, this study introduced the Multi Criteria Vegetation Ecological Quality Index (VEQI), coupled with vegetation cover and vegetation ecological function indicators, to explore the driving factors of vegetation quality in the middle reaches of the Yangtze River and identify key areas where vegetation quality declines or improves. By constructing a Bayesian network for VEQI, we identified the driving variables that influence the index. Additionally, we delineated spatial optimization zones for VEQI. The results indicate that the VEQI exhibits a trend of transitioning from low values in urban centers to high values in suburban and rural areas. Over 20 years, the average VEQI of the study region ranged from 10.85% to 94.94%. Slope, DEM, and vegetation type were identified as significant drivers of VEQI, while precipitation, temperature, and nighttime light were considered secondary factors. Notably, areas in Hunan, Jiangxi, and Hubei provinces, especially the western part of Hunan, were pinpointed as spatial optimization regions. This research not only enhances the understanding of vegetation’s ecological quality in the urban agglomeration of the middle reaches of the Yangtze River but also provides scientific insights for the protection and management of vegetation.
Diagnosis of Two Unrelated Syndromes of Prader-Willi and Calpainopathy: Insight from Trio Whole Genome Analysis and Isodisomy Mapping
Authors: Cuk Mario , Unal Busra , Bevanda Andjela , Hayes Connor P. , Walker McKenzie , Abraamyan Feruza , Beluzic Robert , Gornik Kristina Crkvenac , Ozretic David , Prutki Maja , Nie Qian , Reddi Honey V. , Ghazani Arezou A.
Published: July 2024

Purpose: An investigation for the co-occurrence of two unrelated genetic disorders of muscular dystrophy and Prader-Willi syndrome (PWS) (OMIM#176270) using joint whole genome sequencing (WGS). Methods: Trio WGS joint analysis was performed to investigate the genetic etiology in a proband with PWS, prolonged muscular hypotonia associated hyperCKemia, and early-onset obesity. The parents were unaffected. Results: Results showed maternal isodisomy uniparental disomy (UPD) in chromosome 15, expanding from 15q11.2 to 15q22.2, including PWS regions at 15q11.2–15q13. Maternal heterodisomy was detected from 15q22.2 to 15q26.3. A pathogenic variant, NM_000070.3(CAPN3):c.550del (p.Thr184fs), was identified at 15q15.1 in a heterozygous state in the mother that was homozygous in the proband due to maternal isodisomy. Conclusion: This is the first study of the concurrent molecular etiology of PWS and calpainopathy (OMIM#253600) in the same patient. This report highlights the utility of joint analysis and the need for the assessment of autosomal recessive disease in regions of isodisomy in patients with complex and unexplained phenotypes.
Social Story Intervention for Training Expected Behaviors among Preschool Children: A Systematic Review and Meta-Analysis
Authors: Zhou Ni , Zhou Li , Ho Cheuk Yu Teresa , McGrath Colman , Wong Hai Ming
Published: July 2024

Promoting appropriate behaviors in early childhood is crucial for children’s future development. This systematic review aimed to explore the efficacy of social story (SS) intervention in teaching expected behaviors among preschool children. A structured search strategy was applied to five online electronic databases. The references were systematically screened in accordance with the PRISMA statements. Randomized or non-randomized controlled studies, as well as single-subject studies, in which SSs served as a behavioral training approach for children aged 2 to 6 years were included. Information related to study design, characteristics of the participants, target behaviors, and implementation of SS intervention was extracted. A meta-analysis was performed using the random-effects model, where similar outcomes were evaluated by similar intervention across multiple studies. Twenty-one studies were identified for qualitative analysis, while two studies formed the basis of the meta-analysis. SS interventions were employed to teach a variety of behaviors among typically developing children as well as those with various disabilities, such as autism, developmental delay, hearing impairments, attention deficit hyperactivity disorder, or other disabilities. The target behaviors included oral health practices, peer interaction, staying on-task, self-regulation, sleep habits, and controlling aggressive behavior during group activities. The SSs were used either alone or combined with other strategies, such as positive reinforcement, music therapy, role play, group discussion, video self-modeling, immediate practices, or additional audio commentary. Most studies reported improvements in appropriate behaviors and/or reductions in unfavorable behaviors. The meta-analysis indicated that children practiced more toothbrushing steps when using SS interventions compared to conventional oral health instruction (Z = 3.60, MD = 0.66, 95%CI 0.30 to 1.02, p < 0.001). SS interventions have the potential to teach target behaviors, particularly toothbrushing behaviors, among preschool children. More well-designed randomized controlled trials are warranted to determine the efficacy of SS interventions among children with various developmental profiles.
Cryptic Taxa Revealed through Combined Analysis of Chromosomes and DNA Barcodes: The Polyommatus ripartii Species Complex in Armenia and NW Iran
Authors: Lukhtanov Vladimir A. , Dantchenko Alexander V.
Published: July 2024

The detection of cryptic species in complexes that have undergone recent speciation is often difficult, since many standard nuclear markers have not yet accumulated differences between closely related taxa, and differences in mitochondrial markers can be leveled out due to mitochondrial introgressions. In these cases, the use of derived chromosomal characters such as non-ancestral chromosomal numbers and/or unusual karyotype features may be a solution to the species delimitation problem. However, non-ancestral but similar karyotypes may arise secondarily as a result of homoplastic evolution, and their interpretation as homologies may lead to incorrect taxonomic conclusions. In our study, we show that the combined use of mitochondrial DNA barcodes and karyotypes helps to solve this problem and identifies cryptic species in situations where each of these markers does not work individually. Using this approach, we show that the fauna of Armenia and adjacent Iran includes the following cryptic taxa of the Polyommatus ripartii species complex (haploid chromosome number, n in parentheses): P. ripartii paralcestis (n = 90), P. ripartii kalashiani, subsp. nov (n close to 90), P. emmeli, sp. nov. (n = 77–79), P. keleybaricus, sp. nov. (n = 86), P. demavendi belovi (n = 73–75), P. demavendi antonius, subsp. nov. (n = 71–73), P. admetus anatoliensis (n = 79) and P. eriwanensis (n = 29–34). Polyommatus admetus yeranyani is synonymized with P. admetus anatoliensis.
Radiological Assessment of Coronal Plane Alignment of the Knee Phenotypes in the Romanian Population
Authors: Dragosloveanu Serban , Capitanu Bogdan-Sorin , Josanu Radu , Vulpe Diana , Cergan Romica , Scheau Cristian
Published: July 2024

Background: The Coronal Plane Alignment of the Knee (CPAK) classification system has been developed as a comprehensive framework delineating nine coronal plane phenotypes, based on arithmetic hip–knee angle (aHKA) and joint line obliquity (JLO). Our study aimed to assess the prevalence of knee phenotypes in the Romanian population using the CPAK classification, encompassing both osteoarthritic and healthy cohorts. Methods: We conducted an observational cross-sectional study, analyzing data from 500 knees with osteoarthritis and 500 healthy knees that met the inclusion criteria. Demographic data were collected, and radiological parameters including lateral distal femoral angle (LDFA), medial proximal tibial angle (MPTA), aHKA, and JLO were measured. Knee phenotypes were categorized using the CPAK classification. Results: In the osteoarthritic cohort, the most prevalent CPAK phenotype was type I (42.4%), characterized by varus alignment and an apex distal joint. Conversely, in the healthy population, CPAK type II, indicating neutral alignment and an apex distal joint, was the most prevalent phenotype (39.0%). CPAK types VII, VIII, and IX were rare. Conclusions: Our findings demonstrate similarities in knee phenotypes compared to other populations, with some minor differences and particularities. The CPAK classification proves to be a valuable tool in assessing knee tyalignment.
An Approach for Anomaly Detection in Network Communications Using k-Path Analysis
Authors: Kasse Mamadou , Charrier Rodolphe , Berred Alexandre , Bertelle Cyrille , Delpierre Christophe
Published: July 2024

In this paper, we present an innovative approach inspired by the Path-scan model to detect paths with k adjacent edges (k-path) exhibiting unusual behavior (synonymous with anomaly) within network communications. This work is motivated by the challenge of identifying malicious activities carried out in vulnerable k-path in a small to medium-sized computer network. Each observed edge (time series of the number of events or the number of packets exchanged between two computers in the network) is modeled using the three-state observed Markov model, as opposed to the Path-scan model which uses a two-state model (active state and inactive state), to establish baselines of behavior in order to detect anomalies. This model captures the typical behavior of network communications, as well as patterns of suspicious activity, such as those associated with brute force attacks. We take a perspective by analyzing each vulnerable k-path, enabling the accurate detection of anomalies on the k-path. Using this approach, our method aims to enhance the detection of suspicious activities in computer networks, thus providing a more robust and accurate solution to ensure the security of computer systems.