bluuebunny/crossref_metadata_2025 huggingface dataset:
Multiple functions of the herpesvirus UL14 gene product in viral infection
Authors: Wan Jieyu , Wang Mingshu , Cheng Anchun , Zhang Wei , Yang Qiao , Tian Bin , Ou Xumin , Sun Di , He Yu , Zhao Xinxin , Wu Ying , Zhang Shaqiu , Huang Juan , Wu Zhen , Yu Yanling , Zhang Ling , Zhu Dekang , Liu Mafeng , Chen Shun , Jia Renyong
Published: October 2024

Herpesviruses are a family of double-stranded DNA viruses with a tegument structure and a genome composed of a single sequence and terminal repeat (TR) sequences. The herpesvirus UL14 gene encodes the protein UL14 (pUL14), which has various subcellular localizations and plays a vital role in regulating immediate–early (IE) gene transcription and expression, influences the intracellular localization patterns of several proteins belonging to the capsid and the DNA packaging machinery, participates in secondary envelopment, and influences viral particle release. Additionally, pUL14 has roles in maintaining cellular homeostasis and preventing apoptosis. This review discusses how pUL14 engages in the life cycle of herpesviruses and provides new ideas for further research on pUL14’s function in viral infection.
Case Report: All that glitters is not cancer; perihepatic hibernoma with fluctuating FDG uptake on PET/CT
Authors: Ramzan Amaila , Chander Amarjot , Westwood Thomas , Elias Mark , Manoharan Prakash
Published: October 2024

Hibernomas are rare brown fat tumors that garnered attention in the literature with the increasing use of [18F] Fluorodeoxyglucose Positron Emission Tomography/Computed Tomography ([18F] FDG PET/CT) for the staging workup and follow-up of solid malignancies. Despite being benign tumors, they exhibit high metabolic activity due to their thermogenic nature, leading to significant radiotracer uptake on functional imaging. This can pose a challenge in differentiating them from the malignant lesions, especially the fat-containing malignancies such as liposarcoma. Hibernomas are typically found in the thigh, shoulder, back, and neck. Here, we present a unique case of Hibernoma in a patient undergoing PET/CT for melanoma follow-up in an unusual perihepatic location. To the best of the authors’ knowledge, this represents the first reported case of a perihepatic hibernoma in the literature. The report also offers a literature review on hibernomas, including the influence of ambient temperature on their metabolism, diagnostic challenges, management strategies, and reports of hibernomas detected on functional imaging with a range of radiotracers. These observations could serve as a valuable clue in identifying hibernomas, potentially aiding in avoiding unnecessary biopsies or resections.
Ultrasound analysis of different forms of hemolytic uremic syndrome in children
Authors: Rink Lydia , Finkelberg Ilja , Kreuzer Martin , Schipper Lukas , Pape Lars , Cetiner Metin
Published: October 2024

Background

Hemolytic uremic syndrome (HUS) is the most common cause of acute kidney injury in children. It is mainly caused by Shiga toxin-producing enterohemorrhagicEscherichia coli(EHEC; STEC-HUS) and is more rarely caused by uncontrolled complement activation (cHUS). Renal replacement therapy is frequently required and kidney function recovers in the majority of patients. Ultrasound (US) is the preferred imaging modality for the evaluation of any renal failure. The aim of this study is the evaluation of US diagnostics in both HUS types at disease onset and in the course of the disease.

Materials and methods

Clinical, laboratory, and US data from the digital patient records of children admitted as inpatients with a diagnosis of HUS were recruited for a monocentric, retrospective analysis. STEC-HUS and cHUS were diagnosed when, in addition to the laboratory constellation, EHEC infection and complement system activation were verified, respectively. US examinations were performed by pediatricians with certified pediatric US experience.

Results

In total, 30 children with STEC-HUS (13/25 male; median age of disease onset 2.9 years; most prevalent EHEC serotype was O157) and cHUS (2/5 male; median age of disease onset 5.4 years; 3/5 with proven pathogenic variation) were included. Renal replacement therapy proportions were comparable in the STEC-HUS and cHUS patients (64% vs. 60%). The resistance index (RI) was elevated at disease onset in the patients with STEC-HUS and cHUS (0.88 ± 0.10 vs. 0.77 ± 0.04,p = 0.13) and was similar in the STEC-HUS subcohorts divided based on dialysis requirement (yes: 0.86 ± 0.1; no: 0.88 ± 0.1;p = 0.74). Total kidney size at disease onset displayed a positive correlation with dialysis duration (R = 0.53,p = 0.02) and was elevated in both HUS types (177% ± 56 and 167% ± 53). It was significantly higher in the STEC-HUS subcohort which required dialysis (200.7% vs. 145%,p < .029), and a regressor kidney size threshold value of 141% was indicated in the receiver operating characteristic analysis. A classification model using both US parameters sequentially might be of clinical use for predicting the need for dialysis in patients with STEC-HUS. The US parameters normalized over time.

Conclusion

The US parameters of RI and total kidney size are valuable for the assessment of HUS at disease onset and during therapy, and may be helpful in the assessment of whether dialysis is required in patients with STEC-HUS.
Neurodevelopmental outcome in children between one and five years after persistent pulmonary hypertension of term and near-term newborns
Authors: Atlan Laetitia , Berthomieu Lionel , Karsenty Caroline , Gascoin Géraldine , Arnaud Catherine , Breinig Sophie
Published: October 2024

Background

Persistent pulmonary hypertension of the newborn (PPHN) is a serious condition that affects 1–2 per 1,000 newborns. Scientific data report the existence of neurological developmental abnormalities between 10 and 30%, but the description of these disorders linked with this situation of cerebral hypoxia and haemodynamic failure remains poorly documented.

Objective

The main goal of this study was to describe the prevalence of neuro-psychomotor developmental disorders in children aged between one and five years old who have been hospitalised at birth in a neonatal intensive care unit for the management of PPHN.

Methods

All of the newborns ≥34 weeks of gestational age (WGA) with PPHN, treated with inhaled nitric oxide in our neonatal intensive care unit between January 2015 and December 2019 were retrospectively enrolled. An ASQ-3 standardised questionnaire, adapted to the appropriate age (12, 24, 36, 48 and 60 months) was performed by the parents.

Results

Fifty-five children (81% of answers) with a median age of 36 months (11–68), whose real age was close to the one of the questionnaire (12, 24, 36, 48 and 60 months), have been included in this study. There was 47% of pathological score [borderline: less than 1 standard deviation (SD) or suspect: less than 2SD] in at least one of the five studied domains, mainly in communication (25%) and individual and social skills (22%), despite a high overall score of 250 [220; 285] out of 300 that improved with age.

Conclusion

This study showed a significant prevalence of neuro-psychomotor developmental disorders which justifies making more accessible a prolonged and adapted follow-up for early and multidisciplinary screening and management of these children with PPHN history. Larger cohorts are needed to better explore long term outcome of these vulnerable term neonates.
SPAG6 overexpression decreases the pro-apoptotic effect of daunorubicin in acute myeloid leukemia cells through the ROS/JNK MAPK axis in a GSTP1-dependent manner
Authors: Luo Jie , Ding Li , Pan Shirui , Luo Jing , Zhao Haiqiu , Yin Jiaxiu , Su Rong , Zhang Jiamin , Liu Lin
Published: October 2024

Introduction

As a malignant hematological disease, the incidence of acute myeloid leukemia (AML) has exhibited an upward trend in recent years. Nevertheless, certain limitations persist in the treatment of AML. Sperm-associated antigen 6 (SPAG6) has been implicated in the onset and progression of various human cancers, with its expression levels significantly elevated in AML. Consequently, we undertook a series of experiments to investigate the role and underlying mechanisms of SPAG6 in AML cell lines.

Methods

In the in vitro experiments of this study, DEPs and GO and KEGG enrichment analysis subsequent to SPAG6 down-regulation were detected by TMT. CCK8 was employed to determine cell viability. The levels of apoptosis and ROS were measured by flow cytometry. In the in vivo experiments, a xenografted tumor model was constructed, and the expression of SPAG6 and GSTP1 in tumor tissues was detected by IHC.

Results

Ultimately, our findings indicated that over-expression of SPAG6 promoted cell growth and decreased reactive oxygen species (ROS) and malondialdehyde levels. Furthermore, SPAG6 knockdown was found to diminish mitochondrial membrane potential and facilitate cell apoptosis. In vivo, SPAG6 could also promote tumor growth, suggesting that SPAG6 may serve as a pro-tumor factor. In addition, daunorubicin (DNR) may cause oxidative stress and initiate apoptosis, resulting in oxidative damage to AML cells. However, the overexpression of SPAG6 may attenuate the efficacy of DNR. This was due to SPAG6 promoted GSTP1 expression, thereby reducing ROS levels. Simultaneously, the elevation of GSTP1 and JNK complex may reduce the expression of p-JNK and inhibit the activation of JNK pathway, which might inhibit cell apoptosis.

Discussion

In conclusion, our experiments suggested that upregulated SPAG6 might mitigate the pro-apoptotic effects of DNR through ROS/JNK MAPK axis in a GSTP1-dependent manner.
Ramadan fasting and exercise combination therapy: A novel approach for osteoporosis prevention in ovariectomized rats
Authors: Albrahim Tarfa , Alangry Raghad , Alotaibi Raghad , Almandil Leen , Alburikan Sara , Aloudah Hisham S. , Alahmed Mohammed , Alonazi Mona
Published: October 2024

Background

Osteoporosis is a chronic bone metabolic disease that affects millions of people worldwide, particularly the elderly and postmenopausal women. It is characterized by weakened bones, increasing the risk of fractures and leading to significant morbidity and mortality. The goal of the current study is to examine the reported osteo-preservative effects of exercise and/or fasting in the Ramadan fasting model (RFM) in ovariectomized (OVX) rats.

Methods

The experimental intervention started 1 month following the ovariectomy procedure and consisted of five 15-min exercise sessions per week at 18–25 m/min and/or an approximately 13-h fast from sunrise to sunset (6:00 AM–19:00 PM). Serum bone metabolism biomarker levels were measured, and mineral concentrations in femoral ashed bones and digested serum were determined. Additionally, serum bone alkaline phosphatase (b-ALP), parathyroid hormone, osteocalcin, calcitonin, and vitamin D3 concentrations were measured using the competitive enzyme immunoassay technique.

Results

Calcium, magnesium, and phosphorus showed a notable decrease in mineral concentration among OVX rat femurs compared with the combination group (OVX + RFM + E) and control groups. In addition, homeostasis of serum concentrations of calcium, magnesium, and phosphorus was observed to increase in the OVX + RFM + E group rather than in the OVX group without intervention when compared with a control group. Furthermore, fasting and exercise, either alone or concurrently with ovariectomy, induced a non-significant elevation in osteocalcin, parathyroid hormone, and vitamin D3, whereas b-ALP and calcitonin increased significantly compared with those in control rats.

Conclusion

The combination of the Ramadan fasting model and moderate intensity exercises among OVX rats manifested advantageous effects in bone biomarkers compared with OVX rats without intervention. This could be recommended as a lifestyle modification that is protective against osteoporosis, especially in the context of depleted estrogen hormone after menopause.
Behavioral trace data in an online learning environment as indicators of learning engagement in university students
Authors: Winter Marc , Mordel Julia , Mendzheritskaya Julia , Biedermann Daniel , Ciordas-Hertel George-Petru , Hahnel Carolin , Bengs Daniel , Wolter Ilka , Goldhammer Frank , Drachsler Hendrik , Artelt Cordula , Horz Holger
Published: October 2024

Learning in asynchronous online settings (AOSs) is challenging for university students. However, the construct of learning engagement (LE) represents a possible lever to identify and reduce challenges while learning online, especially, in AOSs. Learning analytics provides a fruitful framework to analyze students' learning processes and LE via trace data. The study, therefore, addresses the questions of whether LE can be modeled with the sub-dimensions of effort, attention, and content interest and by which trace data, derived from behavior within an AOS, these facets of LE are represented in self-reports. Participants were 764 university students attending an AOS. The results of best-subset regression analysis show that a model combining multiple indicators can account for a proportion of the variance in students' LE (highly significant R2 between 0.04 and 0.13). The identified set of indicators is stable over time supporting the transferability to similar learning contexts. The results of this study can contribute to both research on learning processes in AOSs in higher education and the application of learning analytics in university teaching (e.g., modeling automated feedback).
Analyzing assisted reproductive treatment representations in Italy and Spain through newspapers
Authors: Vizzini Marta Anna Stella , Monaco Silvia , Tetecher Loredana , Cappadonna Claudia , Ambriola Vincenzo , Di Trani Michela , Mariani Rachele
Published: October 2024

With the increase in infertility cases recorded over the last 20 years, there is a considerable demand for assisted reproductive treatments (ART). However, there is significant variation in the availability of such treatments across different countries. Legislation on assisted reproduction is influenced by various cultural expressions, making it seemingly impossible to create a single representation adaptable to different contexts. This work investigates the cultural representations of ART in Italy and Spain. We collected 1,735 articles from two Italian and Spanish newspapers, with all the articles containing the respective translation of ART from 2013 to 2022. The two corpora were analyzed using the Emotional Text Mining (ETM) methodology. The analysis produced 3 clusters in the Italian corpus and 5 clusters in the Spanish corpus. From the Italian results, a view of ART emerged that is linked to ethical limitations and the ideal of the traditional family. In contrast, the Spanish results depict ART in terms of community, rights, public health, and birth seen in itself. In conclusion, this study highlights the strong differences between Italian and Spanish cultures regarding ART. The results could be used to improve clinical practices and legislation surrounding ART.
Plant Photosynthetic and Respiration Rates Are Key Populational Traits to Improve Yield and Quality for Good-Tasting Double-Cropped Rice
Authors: Huang Shan , Wu Jiaojiao , Zeng Yongjun , Huang Guanjun
Published: October 2024

Improving the yield and quality for tasty rice varieties is a great challenge. In the present study, different nitrogen rates and plant density were utilized to form differential rice populational structures, which were determined to clarify key traits determining grain yield and quality for tasty rice varieties in a double-cropped rice system in subtropical China. The present results showed that the plant photosynthetic rate, leaf area index and plant respiration rate had important and significant impacts on the grain yields of both early and late rice, though the late rice yield was also significantly affected by the canopy temperature. In addition, among the studied populational traits, plant photosynthetic and/or respiration rates had significant effects on all quality traits. Consistently, grain yield and quality were significantly improved with the increasing plant photosynthetic and respiration rates through correlative analysis, which was also observed in principal components analysis. Overall, the present study suggests that both the grain yield and milling and appearance qualities could be improved through the optimal management of nitrogen and plant density through increasing plant photosynthetic and respiration rates.
Concept of a Cyber–Physical System for Control of a Self-Cleaning Aquaponic Unit
Authors: Dimitrov Kristiyan , Chivarov Nayden , Chivarov Stefan , Paunova-Krasteva Tsvetelina , Filipov Emil , Daskalova Albena
Published: October 2024

The article aims to present a cyber–physical system (CPS) to support the cultivation of aquaculture in a closed aquaponic system using the deep-water culture (DWC) method. The CPS uses precision sensors as TriOxmatic 700 IQ (for dissolved oxygen and water temperature), AmmoLyt Plus 700 IQ (for ammonium), NiCaVis 701 IQ NI (for nitrites and nitrates), SensoLyt® 700 IQ (for pH), and SL-M5 (for water level). It is built with a Raspberry Pi 4, 8 GB as a server, OpenHAB 3.0 software, and other specialized software for measuring water parameters. Some of the parameters are maintained completely autonomously, while others are indirectly controlled. Basic knowledge of hydroponics and aquaculture is required to set up the system, but day-to-day maintenance can be carried out by employees who receive instructions from the CPS. A method for the physical modification of the fish tank surface by using laser processing is proposed. This results in a change in surface topography (creating diverse microstructure patterns) and its roughness, which is of crucial importance for the bacterial adhesion mechanism.
Designing an Economical Water Harvesting System Using a Tank with Numerical Simulation Model WASH_2D
Authors: Nana Jean Bosco , Abd El Baki Hassan M. , Fujimaki Haruyuki
Published: October 2024

Newly incorporated module into the WASH_2D model has enabled simulating a rainwater harvesting system (RWHS) using a tank. The incorporated module in WASH_2D was tested for two field experiments to determine the optimal tank capacity and cultivated area that give the highest net income for farmers. The first experiment was composed of treatments A, B, and C having the same cultivated and harvested areas (plastic sheets) of 24 m2 and 12.5 m2, respectively. The capacity of the tanks for treatments A, B, and C was set at 500, 300, and 200 L, corresponding to storability of 21, 13, and 8 mm, respectively, while in the second experiment we carried out three treatments: F, G, and H having the same tank capacity of 300 L and harvested area of 12.5 m2 with variable cultivated areas as G and H were larger by two and three times than F (10.5 m2), respectively. Water was applied automatically through a drip irrigation system by monitoring soil water suction. Results of the first experiment showed that the optimal storability and seasonal net income simulated by WASH_2D were 17 mm and 5.82 USD yr−1, which were fairly close to 18 mm and 5.75 USD yr−1 observed from field data, respectively. Similarly, the results of the second experiment revealed that simulated net incomes for different cultivated areas agreed well with the observed data. We concluded that the use of the simulation model WASH_2D can be economically useful to promote small-scale irrigation in semi-arid regions and guide planning irrigation or rainwater harvesting investments.
A Novel Interpolation Method for Soil Parameters Combining RBF Neural Network and IDW in the Pearl River Delta
Authors: Zhao Zuoxi , Luo Shuyuan , Zhao Xuanxuan , Zhang Jiaxing , Li Shanda , Luo Yangfan , Dai Jiuxiang
Published: October 2024

Soil fertility is a critical factor in agricultural production, directly impacting crop growth, yield, and quality. To achieve precise agricultural management, accurate spatial interpolation of soil parameters is essential. This study developed a new interpolation prediction framework that combines Radial Basis Function (RBF) neural networks with Inverse Distance Weighting (IDW), termed the IDW-RBFNN. This framework initially uses the IDW method to apply preliminary weights based on distance to the data points, which are then used as input for the RBF neural network to form a training dataset. Subsequently, the RBF neural network further trains on these data to refine the interpolation results, achieving more precise spatial data interpolation. We compared the interpolation prediction accuracy of the IDW-RBFNN framework with ordinary Kriging (OK) and RBF methods under three different parameter settings. Ultimately, the IDW-RBFNN demonstrated lower error rates in terms of RMSE and MRE compared to direct RBF interpolation methods when adjusting settings based on different power values, even with a fixed number of data samples. As the sample size decreases, the interpolation accuracy of OK and RBF methods is significantly affected, while the error of IDW-RBFNN remains relatively low. Considering both interpolation accuracy and resource limitations, we recommend using the IDW-RBFNN method (p = 2) with at least 60 samples as the minimum sampling density to ensure high interpolation accuracy under resource constraints. Our method overcomes limitations of existing approaches that use fixed steady-state distance decay parameters, providing an effective tool for soil fertility monitoring in delta regions.
Mulching Improves the Soil Hydrothermal Environment, Soil Aggregate Content, and Potato Yield in Dry Farmland
Authors: Ma Zhen , Ma Jiantao , Chai Yuwei , Song Wenhuan , Han Fanxiang , Huang Caixia , Cheng Hongbo , Chang Lei
Published: October 2024

Mulching could effectively improve the soil hydrothermal environment, improve changes in the soil structure, increase entropy, and conserve soil moisture to solve the problem of grain reduction caused by perennial drought in Northwest China. Thus, a two-growing-season field experiment (2021–2022) with five treatments (PM1, biodegradable plastic film mulching; PM2, plastic film mulching; SM1, straw strip mulching; SM2, crushed corn straw full mulching; and CK, no mulching as the control) was conducted to investigate the effects of different mulching materials on the soil hydrothermal environment, soil aggregate distribution, stability, and tuber yield of rainfed potato farmland in Northwest China. Over two growing seasons, mulching planting, on average, increased (p < 0.05) the soil moisture at the 0–200 cm depth by 9.0% relative to CK (SM2 (11.6%) > SM1 (10.3%) > PM2 (8.6%) > PM1 (7.0%)). The mulching treatments significantly regulated the soil temperature during the whole growth period, in which plastic mulching significantly increased the soil temperature of the 0–25 cm soil depth during the whole growth period by 2.1 °C (PM2 (2.1 °C) > PM1 (2.0 °C)); meanwhile, straw mulching significantly reduced the soil temperature by 1.4 °C (SM2 (0.9 °C) > SM1 (0.6 °C)). All mulching treatments improved the soil macroaggregate content and soil aggregate stability in all soil depths from 0 to 40 cm, with increases of 31.4% and 27.1% in the mean weight diameter (MWD) and 22.6% and 21.2% in the geometric mean diameter (GWD) compared with CK, respectively. Straw and plastic mulching significantly increased the fresh tuber yield by 12.5% and 12.6% compared with CK, respectively. The increases were greatest in SM2 and PM2. Crushed corn straw full mulching is difficult to sow and harvest; therefore, straw strip mulching could improve the soil hydrothermal environment, increase production, and provide an environmentally friendly technology for dryland potato production.
Influence of Vine Decline Disease on the Amino Acid Metabolism of Watermelon Fruit
Authors: Santo Honoka , Tadano Shota , Inokami Fumika , Nishioka Takuya , Konaka Takafumi , Sakata Motomu , Morimoto Yasufumi , Akashi Kinya
Published: October 2024

Vine decline (VD) is a recalcitrant syndrome of watermelon, melon, and other cucurbits, often associated with soil-borne pathogens such as Monosporascus cannonballus and characterized by root necrosis, leaf chlorosis, and wilting at the later stage of fruit maturation. The present study examined VD’s effects on watermelon fruits’ metabolism. The VD-affected watermelon fruits had significantly lower lycopene and total solid contents. Still, polyphenols content and total antioxidant activities were comparable with the controls, suggesting that VD inhibited the ripening processes but maintained defensive processes in the fruits. The VD fruits showed a lower calcium level than the controls, while the contents of other major nutrition minerals were not significantly altered. The VD fruits had a lower content of total amino acids, and their composition was characterized by an increase in the percentage fractions for several amino acids, including citrulline, which may reflect the physiological response to the VD-related water deficit condition. The principal component analysis distinguished amino acid profiles between the VD and control fruits, demonstrating that VD significantly influenced their amino acid metabolisms. The present study revealed that VD imposed characteristic impacts on the biochemical behaviors in the watermelon fruits.
Application of Path Planning and Tracking Control Technology in Mower Robots
Authors: Liu Lixing , Liu Hongjie , Wang Xu , Li Jianping , Wang Pengfei , Liu Shuteng , Zou Jinxuan , Yang Xin
Published: October 2024

Path planning and tracking is the most basic technology for mowing robots, among which the performance of algorithms has a great impact on their intelligence and efficiency. Based on the research of relevant references on mower robots, it mainly focuses on complete coverage path planning, path tracking control, and obstacle avoidance path planning. In complete coverage path planning, three methods were introduced, including simple complete coverage planning, optimal complete coverage planning, and hybrid complete coverage planning. In the path tracking control section, the control methods are divided into three types based on whether the control method depends on the robot model and the type of model, namely model free control method, kinematic model-based control method, and dynamic model-based control method. In obstacle avoidance path planning, we introduce the environment detection device and obstacle avoidance planning algorithm. Then the relevant research papers are analyzed in classification, comparing the research and validation methods adopted by the researchers in the form of charts. Finally, we pointed out the limitations of path planning technology in the application of mower robots. Meanwhile, future development trends are predicted.
Organic Fertilization Improves Soil Multifunctionality in Sugarcane Agroecosystems
Authors: Delmotte Sacha , Brunel Caroline , Castanier Louise , Fevrier Amélie , Brauman Alain , Versini Antoine
Published: October 2024

Soil multifunctionality is closely tied to soil health, yet a comprehensive understanding of this link in agricultural soils is lacking. The aim of this study was to understand how long-term fertilization practices affect the provision of multiple services by comparing the multifunctionality of soils. The three objectives were to (i) determine whether the effect of fertilization is consistent across soil types, (ii) describe the effect of the different fertilizers on soil multifunctionality, and (iii) identify soil chemical properties that can be easily used proxies of soil multifunctionality. The descriptors belong to three functioning indexes associated with nutrient availability, carbon transformation, and soil structure maintenance. This study is the first to investigate the effect of a variety of organic fertilizers on the health of three soil types by combining physical, chemical, and biological indicators in sugarcane agroecosystems. An increase in soil multifunctionality was obtained, with no effect on yield. The effect of fertilizers was consistent across soil types. Filter mud and green waste compost significantly increased the multifunctionality and functioning indexes compared to mineral fertilizer. Modifications in soil properties did not fully explain the observed variations. Our results confirm the high potential of organic fertilization to improve multifunctionality and provide ecosystem services.
Bioactive Adrenomedullin in Dogs with Sepsis and Septic Shock: A Prospective, Case-Control Study
Authors: Chrysovergi Eirini , Mooney Carmel T. , Shiel Robert E. , Stavroulaki Evangelia M. , Murtagh Kevin
Published: October 2024

Sepsis and septic shock are leading causes of morbidity and mortality in both humans and dogs, and early diagnosis is crucial for improving outcomes. This study aimed to evaluate bioactive adrenomedullin (bio-ADM) concentrations in dogs with septic shock (n = 25), dogs with sepsis without evidence of shock (n = 25), and healthy control dogs (n = 25). Plasma bio-ADM concentrations were measured using a human sandwich enzyme-linked immunosorbent assay and reported as median (interquartile range). Plasma bio-ADM concentrations were significantly higher in both septic groups compared to the healthy controls (all <22.4 pg/mL), but not significantly different between the septic shock (75.0 [28.7–115.0] pg/mL) and sepsis (30.7 [22.4–79.7] pg/mL) groups. Dogs with higher illness severity scores had significantly higher bio-ADM concentrations (93.1 [32.2–122.0] pg/mL) than those with lower scores (29.8 [22.4–71.2] pg/mL). However, bio-ADM concentrations did not differ between survivors (33.0 [22.7–76.7] pg/mL) and non-survivors (74.7 [26.1–123.2] pg/mL). Measurement of bio-ADM is a potential marker for canine sepsis, but not for the identification of septic shock, and may provide information on disease severity. Further studies, including those on non-infectious inflammatory conditions, are necessary to better understand the diagnostic utility of bio-ADM measurement and its potential role as a marker of treatment response in dogs with sepsis.
Modeling and Regulation of Dynamic Temperature for Layer Houses Under Combined Positive- and Negative-Pressure Ventilation
Authors: Li Lihua , Li Min , Yu Yao , Jia Yuchen , Qian Zhengkai , Xie Zongkui
Published: October 2024

The environmental control of layer houses with multi-tiered cage systems is influenced by factors such as the structure of the henhouses and the heat dissipation of the flock, leading to low precision and large fluctuations in temperature control. Based on a new combined positive- and negative-pressure ventilation (CPNPV) mode, a dynamic temperature model is constructed. Additionally, a temperature control method for a layer house is designed using a variable universe fuzzy PID control algorithm (VFPID). First, based on the principles of energy and mass balance, and by decoupling the relationship between positive- and negative-pressure ventilation volumes, a dynamic temperature model for layer houses under CPNPV was established. Then, the PID parameters and the proportional relationship between positive- and negative-pressure ventilation were optimized through fuzzy rules, and a proportional exponential function was introduced to adjust the scaling of the universe, enabling fine-tuned control. Finally, a temperature control model for the layer house was built using Simulink. The results show that the coefficients of determination (R2) of the constructed dynamic temperature models are between 0.79 and 0.88, respectively, indicating high accuracy. The designed VFPID method outperformed traditional on–off control and improved control precision by 20–23.53% and 10.34–22.22% compared with PID control and fuzzy PID(FPID) control methods, respectively. This study provides new insights for the development of environmental control equipment and precise environmental regulation of layer houses.
Genomic Insights and Conservation Priorities for Kongshan Cattle: A Whole-Genome Resequencing Approach
Authors: Sun Wenqiang , Ren Hanjun , Li Mengze , Mei Liping , Zhang Bingfei , Jia Xianbo , Chen Shiyi , Wang Jie , Lai Songjia
Published: October 2024

Kongshan Cattle, indigenous to Sichuan Province and recognized as China’s 56th local cattle breed in 2024, exhibit unique adaptations including superior resistance to harsh conditions. Despite a declining population due to the influx of foreign breeds, there is a significant focus on preserving their genetic diversity through advanced genomic techniques. This study utilized whole-genome resequencing, a cost-effective and information-rich method, to perform a comprehensive genetic assessment of the Kongshan Cattle. High-quality resequencing data yielded an average of 17.5 billion clean bases per sample, with high proportions of Q20 and Q30 bases and a balanced GC content. SNP analysis revealed an average of 14 million SNPs per sample, with a notable transition-to-transversion ratio and a significant portion of heterozygosity. Further analysis of genomic and coding regions identified substantial insertions and deletions, particularly in coding sequences affecting gene functionality. A detailed examination of these genetic variations highlighted genes, including NEIL2 and PNKP, which are integral to stress resistance pathways, indicating potential adaptive advantages. This study not only underscores the genetic diversity of Kongshan Cattle but also contributes to broader efforts in germplasm conservation.
Study on the Differences in Fecal Metabolites and Microbial Diversity of Jiangshan Black-Bone Chickens with Different Earlobe Colors
Authors: Wang Zhijun , Li Shiru , Ding Xiangying , Du Xue , Zhao Ayong
Published: October 2024

The differences in earlobe color of Jiangshan black-bone chickens have been reported to be caused by the combined effects of melanin and collagen. In this study, we conducted LC-MS untargeted metabolomics and 16S rDNA diversity sequencing on the cecal contents of two types of earlobes: peacock green (Blue and Green group) and dark reddish-purple (Black group). The metabolomic sequencing identified a total of 747 differential metabolites (DMs), in which the metabolites were primarily enriched in tyrosine and tryptophan metabolism pathways between peacock green and dark reddish-purple earlobes. There were 15 different bacterial taxa among the three groups of earlobes at the genus level, and correlation analysis between metabolites and microbes revealed that the DMs between peacock green and dark reddish-purple earlobes were positively correlated with the different bacterial taxa. In short, there are differences in gut microbiota and metabolites between Jiangshan black-bone chickens with peacock green earlobes and those with dark reddish-purple earlobes. Our results suggest that the bacterial phyla Firmicutes and Bacteroidota may influence melanin synthesis by affecting tryptophan metabolism, induced by 5-Methoxyindoleacetate, and tyrosine metabolism, induced by maleylacetoacetic acid and maleic acid, leading to differences in earlobe color.
Improving Outcomes and Preventing Complications in Cranial Base Surgery
Authors: Montano Nicola , Martinelli Renata , Almeida Joao Paulo
Published: October 2024

Skull base surgery has evolved remarkably since the pioneering techniques of early 20th-century surgeons, such as Schloffer and Cushing, who laid the foundation for transcranial and transnasal approaches [...]
The Enigma That Is ROHHAD Syndrome: Challenges and Future Strategies
Authors: Hawton Katherine , Giri Dinesh , Crowne Elizabeth , Greenwood Rosemary , Hamilton-Shield Julian
Published: October 2024

Rapid-onset obesity with hypoventilation, hypothalamic dysfunction, and autonomic dysregulation (ROHHAD) is a rare syndrome presenting in early childhood associated with a high risk of mortality between 50 and 60%. It is characterised by rapid, early onset of obesity between 1.5–7 years, along with central hypoventilation and hypothalamic dysfunction, such as central hypothyroidism, hyperprolactinemia, disorders of sodium and water balance, growth hormone deficiency, adrenocortical insufficiency, or disorders of puberty and features of autonomic dysregulation. Up to half of cases have neural crest tumours, most commonly ganglioneuromas or ganglioneuroblastomas. The incidence of ROHHAD syndrome in any population is unknown. Currently, there is no specific diagnostic or genetic biomarker for ROHHAD, and diagnosis is based on clinical signs and symptoms, which is often challenging, and consequently may be delayed or unrecognised. Early diagnosis is important, as without intervention, ROHHAD is associated with high morbidity and mortality. Aetiology remains unclear; an autoimmune origin has been postulated, with immunosuppressive agents being used with variable benefit. With no cure, multidisciplinary management is largely supportive. Therefore, there are many unanswered questions in ROHHAD syndrome. In this review article, we outline the challenges posed by ROHHAD syndrome, including aetiology, genetics, diagnosis, screening, management, and prognosis. We present research priorities to tackle these issues to improve outcomes.
Individual Differences in the Impact of Distracting Environmental Sounds on the Performance of a Continuous Visual Task in Older Adults
Authors: Richards Leanne , Carter Neil , Hanley Claire J. , Barnes Claire , Summers Huw , Porter Alison , Tales Andrea
Published: October 2024

Background/Objectives: Vulnerability to sound distraction is commonly reported in older adults with dementia and tends to be associated with adverse impacts on daily activity. However, study outcome heterogeneity is increasingly evident, with preserved resistance to distraction also evident. Contributory factors may include individual differences in distractibility in older adulthood per se, and failure to consider the influence of how difficult a person found the test. Methods: We therefore measured distractibility in a group of older adults by comparing the performance of a primary visual task (Swansea Test of Attentional Control), which includes an adaptive algorithm to take into account how difficult a person finds the test under both no-sound and sound conditions. Results: Analysis revealed no significant difference in group mean performance between no-sound versus sound conditions [t (33) = 0.181, p = 0.858; Cohen’s effect size d = −0.028], but individual differences in performance both within and between sound and no-sound conditions were evident, indicating that for older adults, distracting sounds can be neutral, detrimental, or advantageous with respect to visual task performance. It was not possible to determine individual thresholds for whether sound versus no-sound conditions affected a person’s actual behaviour. Conclusions: Nevertheless, our findings indicate how variable such effects may be in older adults, which in turn may help to explain outcome heterogeneity in studies including people living with dementia. Furthermore, such within-group heterogeneity highlights the importance of considering a person’s individual performance in order to better understand their behaviour and initiate interventions as required.
Known-Group Validity and Sensitivity to Change in the Sensory-Motor Dysfunction Questionnaire in Individuals with Neck Pain: A Pilot Study
Authors: Ambalavanar Ushani , McIntosh Megan , Haavik Heidi , Murphy Bernadette
Published: October 2024

Background/Objectives: Sensorimotor dysfunction is often reported in individuals experiencing neck pain, irrespective of severity and chronicity. The treatment of neck dysfunction has been found to positively impact sensorimotor integration (SMI), thereby improving sensorimotor function. However, no patient-reported outcome measure has been validated for discrimination between healthy individuals and neck pain patients with disordered SMI, nor has there been documentation of positive change in SMI following neck pain treatment.The purpose of this study was to administer the Sensory-Motor Dysfunction Questionnaire (SMD-Q), for the purposes of: (1) known-group validity between individuals without and with chronic neck pain (CNP) or subclinical neck pain (SCNP); (2) assess the questionnaire’s capacity to quantify changes in altered SMI following a tailored treatment plan. Methods: Part 1—Known Group Validity: The SMD-Q was administered to 30 neck pain (13 with CNP, and 17 with SCNP), and 30 healthy participants. Part 2—Sensitivity to Change: The SMD-Q was re-administered to neck pain participants following their tailored treatment plans (SCNP—8-weeks and CNP—12 weeks). Results: The SMD-Q can discriminate between healthy and neck pain participants (p ≤ 0.001), and may be sensitive to showing treatment effects (ηp2 = 0.162; large effect size (ES)), but the sample size was too small to determine if it can discriminate treatment effects between groups (ηp2 = 0.070; medium ES). Conclusions: Differing degrees of disordered SMI can be discriminated by the SMD-Q, but further research is needed to determine its sensitivity to treatment.
Exploring the Frontier: The Human Microbiome’s Role in Rare Childhood Neurological Diseases and Epilepsy
Authors: Belnap Newell , Ramsey Keri , Carvalho Sophia T. , Nearman Lexi , Haas Hannah , Huentelman Matt , Lee Keehoon
Published: October 2024

Emerging research into the human microbiome, an intricate ecosystem of microorganisms residing in and on our bodies, reveals that it plays a pivotal role in maintaining our health, highlighting the potential for microbiome-based interventions to prevent, diagnose, treat, and manage a myriad of diseases. The objective of this review is to highlight the importance of microbiome studies in enhancing our understanding of rare genetic epilepsy and related neurological disorders. Studies suggest that the gut microbiome, acting through the gut–brain axis, impacts the development and severity of epileptic conditions in children. Disruptions in microbial composition can affect neurotransmitter systems, inflammatory responses, and immune regulation, which are all critical factors in the pathogenesis of epilepsy. This growing body of evidence points to the potential of microbiome-targeted therapies, such as probiotics or dietary modifications, as innovative approaches to managing epilepsy. By harnessing the power of the microbiome, we stand to develop more effective and personalized treatment strategies for children affected by this disease and other rare neurological diseases.
Moral Believer or Moral Problem-Solver? Moral Pragmatism Fosters Tolerance Without Impeding Moral Behavior
Authors: Zhang Li , Tong Song , Peng Kaiping
Published: October 2024

Many previous studies in moral psychology have described people as moral believers, who treat morality as universal sacred beliefs and show moral outrage and social exclusion toward people with different opinions. At the same time, moral relativism tends to make people more tolerant but also makes them question their own beliefs and leads to more immoral behavior. We propose moral pragmatism as an alternative, which treats morality as a tool for solving specific problems, thus making morality situational instead of universal, practical instead of sacred, and tolerant instead of exclusive. Through four empirical studies, we demonstrate that when moral issues are presented as practical problems rather than abstract beliefs, people consider morality to be less universal, treat dissidents with more tolerance and less outrage, and do not perform more immoral behavior at the same time. These findings highlight moral pragmatism as a flexible and culturally sensitive moral approach, promoting diverse moral perspectives and constructive cross-cultural discourses.
Comparison of Brønsted Acidic Silanol Nests and Lewis Acidic Metal Sites in Ti-Beta Zeolites for Conversion of Butenes
Authors: Yi Fengjiao , Xing Mengjiao , Cao Jing-Pei , Guo Shupeng , Yang Yong
Published: October 2024

The Lewis acidic framework Ti sites in Ti-Beta and Si-Beta catalysts were compared by FT-IR and NMR characterization methods before they were applied to the conversion of four butenes. The results showed that Si-Beta has fewer Lewis acid sites and abundant weak Brønsted acidic silanol nests, which play an important role in conversions between n-butene, cis-2-butene, and trans-2-butene. The conversions for these butenes over Si-Beta were always higher than those over a series of Ti-Beta catalysts with gradient-varied Lewis acidic framework Ti sites and silanols. This is because isobutene can only oligomerize, which requires stronger acidity, so its conversion over Si-Beta was lower than those over Ti-Beta zeolites. For a series of Ti-Beta catalysts with different abundances of Lewis acidic Ti sites, the more Lewis acid sites it had, the higher the conversions for the four butenes.
Development of a Custom Fluid Flow Chamber for Investigating the Effects of Shear Stress on Periodontal Ligament Cells
Authors: Nile Mustafa , Folwaczny Matthias , Kessler Andreas , Wichelhaus Andrea , Janjic Rankovic Mila , Baumert Uwe
Published: October 2024

The periodontal ligament (PDL) is crucial for maintaining the integrity and functionality of tooth-supporting structures. Mechanical forces applied to the tooth during orthodontic tooth movement generate pore pressure gradients, leading to interstitial fluid movement within the PDL. The generated fluid shear stress (FSS) stimulates the remodeling of PDL and alveolar bone. Herein, we present the construction of a parallel fluid-flow apparatus to determine the effect of FSS on PDL cells. The chamber was designed and optimized using computer-aided and computational fluid dynamics software. The chamber was formed by PDMS using a negative molding technique. hPDLCs from two donors were seeded on microscopic slides and exposed to FSS of 6 dyn/cm2 for 1 h. The effect of FSS on gene and protein expression was determined using RT-qPCR and Western blot. FSS upregulated genes responsible for mechanosensing (FOS), tissue formation (RUNX2, VEGFA), and inflammation (PTGS2/COX2, CXCL8/IL8, IL6) in both donors, with donor 2 showing higher gene upregulation. Protein expression of PTGS2/COX2 was higher in donor 2 but not in donor 1. RUNX2 protein was not expressed in either donor after FSS. In summary, FSS is crucial in regulating gene expression linked to PDL remodeling and inflammation, with donor variability potentially affecting outcomes.