bluuebunny/crossref_metadata_2025 huggingface dataset:
Canning Processes Reduce the DNA-Based Traceability of Commercial Tropical Tunas
Authors: Pecoraro Carlo , Crobe Valentina , Ferrari Alice , Piattoni Federica , Sandionigi Anna , Andrews Adam J. , Cariani Alessia , Tinti Fausto
Published: September 2020

Canned tuna is one of the most widely traded seafood products internationally and is of growing demand. There is an increasing concern over the vulnerability of canned tuna supply chains to species mislabelling and fraud. Extensive processing conditions in canning operations can lead to the degradation and fragmentation of DNA, complicating product traceability. We here employed a forensically validated DNA barcoding tool (cytochrome b partial sequences) to assess the effects of canning processes on DNA degradation and the identification of four tropical tuna species (yellowfin, bigeye, skipjack and longtail tuna) collected on a global scale, along their commercial chains. Each species was studied under five different canning processes i.e., freezing, defrosting, cooking, and canning in oil and brine, in order to investigate how these affect DNA-based species identification and traceability. The highest percentage of nucleotide substitutions were observed after brine-canning operations and were greatest for yellowfin and skipjack tuna. Overall, we found that DNA degradation significantly increased along the tuna canning process for most specimens. Consequently, most of the specimens canned in oil or brine were misidentified due to the high rate of nucleotide substitution in diagnostic sequences.
Microencapsulation of Vanilla Oleoresin (V. planifolia Andrews) by Complex Coacervation and Spray Drying: Physicochemical and Microstructural Characterization
Authors: Hernández-Fernández Miguel Ángel , García-Pinilla Santiago , Ocampo-Salinas Oswaldo Israel , Gutiérrez-López Gustavo Fidel , Hernández-Sánchez Humberto , Cornejo-Mazón Maribel , Perea-Flores María de Jesús , Dávila-Ortiz Gloria
Published: September 2020

Vanilla is one of the most popular species in the world. Its main compound, vanillin, is responsible for its characteristic aroma and flavor and its antioxidant and biological properties. Vanillin is very unstable in the presence of oxygen, light, and humidity, which complicates its use and preservation. Therefore, to solve this problem, this study aimed to develop vanilla oleoresin microcapsules. Vanilla oleoresin was obtained with supercritical carbon dioxide and microencapsulated by complex coacervation and subsequent spray drying (100 °C/60 °C inlet/outlet temperature). The optimal conditions for the complex coacervation process were 0.34% chitosan, 1.7% gum Arabic, 5.29 pH, and an oleoresin:wall material ratio of 1:2.5. Fourier Transform Infrared Spectroscopy (FT-IR) analysis of the coacervates before and after spray drying revealed the presence of the functional group C=N (associated with carbonyl groups of vanillin and amino groups of chitosan), indicating that microencapsulation by complex coacervation-spray drying was successful. The retention and encapsulation efficiencies were 84.89 ± 1.94% and 69.20 ± 1.79%. The microcapsules obtained from vanilla oleoresin had high vanillin concentration and the presence of other volatile compounds and essential fatty acids. All this improves the aroma and flavor of the product, increasing its consumption and application in various food matrices.
A Combination of Melatonin and Ethanol Treatment Improves Postharvest Quality in Bitter Melon Fruit
Authors: Lin Xiaoxue , Wang Li , Hou Yuanyuan , Zheng Yonghua , Jin Peng
Published: September 2020

Central composite design (CCD), utilized with three independent variables, verified that the optimal treatment conditions in bitter melon fruit were melatonin (MT) concentration of 120 µmol L−1, ethanol concentration of 6%, and immersing time of 10 min. Under optimal conditions, the experimental values of firmness, chilling injury (CI) index, and weight loss were shown as 27.81 N, 65.625%, and 0.815%, respectively. Moreover, the combined effect of MT and ethanol on CI and physiological quality in postharvest bitter melon fruit stored at 4 °C was investigated. It was found that the combined treatment contributed to the reduced CI symptoms and inhibited ion leakage and malondialdehyde (MDA) accumulation. Moreover, higher levels of chlorophyll, total soluble solids (TSSs), soluble sugar, soluble protein, and ascorbic acid (AsA) were observed in comparison with the control group. Furthermore, the synthesis of total phenols and flavonoids in bitter melon was greatly promoted. Therefore, the combination of MT and ethanol could have the potential for alleviating CI and maintaining postharvest quality for the duration of cold storage.
Unraveling the Physics of Quasar Jets: Optical Polarimetry and Implications for the X-ray Emission Process
Authors: Perlman Eric S. , Clautice Devon , Avachat Sayali , Cara Mihai , Sparks William B. , Georganopoulos Markos , Meyer Eileen
Published: September 2020

Since the launch of Chandra twenty years ago, one of the greatest mysteries surrounding Quasar Jets is the production mechanism for their extremely high X-ray luminosity. Two mechanisms have been proposed. In the first view, the X-ray emission is inverse-Comptonized CMB photons. This view requires a jet that is highly relativistic (bulk Lorentz factor >20–40) on scales of hundreds of kiloparsecs, and a jet that is comparably or more powerful than the black hole’s Eddington luminosity. The second possibility is synchrotron emission from a high-energy population of electrons. This requires a much less powerful jet that does not need to be relativistically beamed, but it imposes other extreme requirements, namely the need to accelerate particles to >100 TeV energies at distances of hundreds of kiloparsecs from the active nucleus. We are exploring these questions using a suite of observations from a diverse group of telescopes, including the Hubble Space Telescope (HST), Chandra X-ray Observatory (CXO), Fermi Gamma-ray Space Telescope and various radio telescope arrays. Our results strongly favor the hypothesis that the X-ray emission is synchrotron radiation from a separate, high-energy electron population. We discuss the observations, results and new questions brought up by these surprising results. We investigate the physical processes and magnetic field structure that may help to accelerate particles to such extreme energies.
Genome-Wide Identification and Characterization of Fusarium graminearum-Responsive lncRNAs in Triticum aestivum
Authors: Duan Xiaoxin , Song Xiushi , Wang Jianxin , Zhou Mingguo
Published: September 2020

Although the war between wheat and Fusarium has been widely investigated for years, long noncoding RNAs (lncRNAs), which have been proven to regulate important processes in the development and stress responses of plants, are still poorly known in wheat against Fusarium. Herein, we systematically reveal the roles of wheat lncRNAs in the process of Fusarium graminearum infection by high-throughput RNA sequencing. Well over 4130 of the total 4276 differentially expressed lncRNAs were already specifically expressed at 12 h postinoculation (hpi), but only 89 of these were specifically expressed at 24 hpi, indicating that the initial stage was the crucial stage for lncRNA-mediated gene regulation of wheat defense against F. graminearum. Target analysis showed the lncRNAs participated in various biological stress processes and had exclusive regulation models at different infection stages. Further H2O2 accumulation and protein ubiquitination assays supported this idea. Moreover, two lncRNAs (XLOC_302848 and XLOC_321638) were identified as Fusarium seedling blight resistance candidates by lncRNA-target expression pattern validation, and two lncRNAs (XLOC_113815, XLOC_123624) were Fusarium head blight resistance potential regulators by cross-validating the RNAseq data with the refined meta-QTL of wheat FHB resistance. These findings extend our knowledge on wheat lncRNAs response to F. graminearum attack and provide new insights for the functional and molecular research of future interactions between wheat and Fusarium.
Features of DNA Repair in the Early Stages of Mammalian Embryonic Development
Authors: Khokhlova Evgenia V. , Fesenko Zoia S. , Sopova Julia V. , Leonova Elena I.
Published: September 2020

Cell repair machinery is responsible for protecting the genome from endogenous and exogenous effects that induce DNA damage. Mutations that occur in somatic cells lead to dysfunction in certain tissues or organs, while a violation of genomic integrity during the embryonic period often leads to death. A mammalian embryo’s ability to respond to damaged DNA and repair it, as well as its sensitivity to specific lesions, is still not well understood. In this review, we combine disparate data on repair processes in the early stages of preimplantation development in mammalian embryos.
Diversity of Volcanic Geoheritage in the Canary Islands, Spain
Authors: Dóniz-Páez Javier , Beltrán-Yanes Esther , Becerra-Ramírez Rafael , Pérez Nemesio M. , Hernández Pedro A. , Hernández William
Published: September 2020

Volcanic areas create spectacular landscapes that contain a great diversity of geoheritage. The study of this geoheritage enables us to inventory, characterise, protect and manage its geodiversity. The Canary Islands are a group of subtropical active volcanic oceanic islands with a great variety of magma types and eruption dynamics that give rise to a wide diversity of volcanic features and processes. The aim of this paper is to identify, for the first time, the diversity of volcanic geoheritage of the Canary Islands and to appraise the protection thereof. To this end, a geomorphological classification is proposed, taking into account the features and processes directly related to volcanism, such as those resulting from erosion and sedimentary processes. The main findings demonstrate that the volcanic geoheritage of the Canary Islands is extremely varied and that this geodiversity is safeguarded by regional, national and, international protection and management frameworks. Even so, and given the enormous pressure of coastal tourism on the coastlines of the islands, we believe that continuing efforts should be made to conserve and manage their volcanic and non-volcanic geoheritage, so that these places can continue to be enjoyed in the form of geotourism.
Factors Predicting the Utilization of Center-Based Cardiac Rehabilitation Program
Authors: Young Lufei , Zhang Qi , Lian Eric , Roberts Kimberly , Weintraub Neal , Dong Yanbin , Zhu Haidong , Xu Hongyan , Schafer Pascha , Dunlap Stephanie
Published: September 2020

Although cardiac rehabilitation (CR) is clearly beneficial to improving patients’ physical functioning and reducing heart disease progression, significant proportions of patients do not complete CR programs. To evaluate the prevalence and predictors of completion of a center-based CR program in eligible cardiac patients, existing data collected from electronic medical records were used. To identify the predictors of CR completion, we used principal components analysis (PCA) and an artificial neural network (ANN) module. Among 685 patients, 61.4% (n = 421) completed the program, 31.7% (n = 217) dropped out, and 6.9% (n = 47) were referred but failed to initiate the program. PCA was conducted to consolidate baseline data into three factors—(1) psychosocial factors (depression, anxiety, and quality of life), (2) age, and (3) BMI, which explained 66.8% of the total variance. The ANN model produced similar results as the PCA. Patients who completed CR sessions had greater extremity strength and flexibility, longer six-minute walk distance, more CR knowledge, and a better quality of life. The present study demonstrated that patients who were older, obese, and who had depression, anxiety, or a low quality of life were less likely to complete the CR program.
Sensitivity of Planktonic Cells of Staphylococcus aureus to Elevated Hydrostatic Pressure as Affected by Mild Heat, Carvacrol, Nisin, and Caprylic Acid
Authors: George Jyothi , Aras Sadiye , Kabir Md Niamul , Wadood Sabrina , Chowdhury Shahid , Fouladkhah Aliyar Cyrus
Published: September 2020

Current study investigated effects of elevated hydrostatic pressure exposure in the presence of mild heat and natural antimicrobials against Staphylococcus aureus. Hydrostatic pressure of 350 to 550 MPa with nisin (5000 IU/mL), carvacrol, or caprylic acid (0.5% v/v) were applied for the reduction in four-strain mixture of S. aureus in HEPES buffer at 4 and 40 °C for up to 7 min. Results were statistically analyzed by ANOVA and D-values were additionally calculated using best-fitted linear model. Prior to exposure to treatments at 4 °C, counts of the pathogen were 7.95 ± 0.4 log CFU/mL and were reduced (p < 0.05) to 6.44 ± 0.3 log CFU/mL after 7 min of treatment at 450 MPa. D-value associated with this treatment was 5.34 min (R2 = 0.72). At 40 °C, counts were 8.21 ± 0.7 and 5.77 ± 0.3 log CFU/mL before and after the 7-min treatments, respectively. D-value associated with 40 °C treatment was 3.30 min (R2 = 0.62). Application of the antimicrobials provided additional pathogen reduction augmentation for treatments < 5 min. The results of the current study could be incorporated for meeting regulatory requirements such as Food Code, HACCP, and Preventive Control for Human Food of Food Safety Modernization Act for assuring microbiological safety of products against this prevalent pathogen of public health concern.
Applying Principles from Prevention and Implementation Sciences to Optimize the Dissemination of Family Feeding Interventions
Authors: Parker Louise , Jackson Alexandra , Lanigan Jane
Published: September 2020

Because families are the primary food socialization agent for children, they are a key target for nutrition interventions promoting healthy eating development. Although researchers and clinicians have developed and tested successful family nutrition interventions, few have gained widespread dissemination. Prevention and implementation science disciplines can inform the design, testing, and dissemination of feeding interventions to advance the goals of widespread adoption and population health impact. We review concepts and frameworks from prevention science and dissemination and implementation (D&I) research that are useful to consider in designing, implementing, and evaluating feeding interventions. Risk and protective factor frameworks, types of translation processes, and implementation dimensions are explained. Specifically, we address how research–practice partnerships can reduce time to dissemination, how designing for modularity can allow for contextual adaptation, how articulating core components can strengthen fidelity and guide adaptation, and how establishing technical assistance infrastructure supports these processes. Finally, we review strategies for building capacity in D&I research and practice for nutrition professionals. In sum, the research and knowledge bases from prevention and implementation sciences offer guidance on designing and delivering family interventions in ways that maximize the potential for their broad dissemination, reducing time to translation and optimizing interventions for real-world settings.
Exploring the Participant-Related Determinants of Simulator Sickness in a Physical Motion Car Rollover Simulation as Measured by the Simulator Sickness Questionnaire
Authors: Rzeźniczek Piotr , Lipiak Agnieszka , Bilski Bartosz , Laudańska-Krzemińska Ida , Cybulski Marcin , Chawłowska Ewelina
Published: September 2020

Physical motion driving simulators serve as a valuable research and training tool. Since many simulator participants suffer from simulator sickness (SS), we aimed to gain a better understanding of participant-related variables that may influence its incidence and severity. The study involved a 2-min mobile-platform car rollover simulation conducted in a group of 100 healthy adult participants. SS was measured with the Simulator Sickness Questionnaire immediately before and after the simulation. We investigated how the symptomatology of SS varies with gender, as well as with participants’ previous experiences such as extra driving training or car accidents. Although many SS symptoms occurred already before the simulation, all the symptoms except burping had a significantly greater incidence and severity after the simulation. Before the simulation, men reported disorientation symptoms more often than women, while participants with prior experiences of extra driving training or car accidents scored significantly higher in three out of four Questionnaire components: nausea symptoms, oculomotor symptoms, and the total score. The study offers interesting insights into associations between SS and prior experiences observed by means of high-fidelity real-motion simulations. More research is needed to determine the nature of these associations and their potential usefulness, for example, in helping accident survivors to cope with the distressing or even potentially disabling psychological consequences of accidents.
Use of Neurodynamic or Orthopedic Tension Tests for the Diagnosis of Lumbar and Lumbosacral Radiculopathies: Study of the Diagnostic Validity
Authors: González Espinosa de los Monteros Francisco Javier , Gonzalez-Medina Gloria , Ardila Elisa Maria Garrido , Mansilla Juan Rodríguez , Expósito José Paz , Ruiz Petronila Oliva
Published: September 2020

Background: Lumbar radiculopathy is a nerve root disorder whose correct diagnosis is essential. The objective of the present study was to analyze the reliability diagnostic validity of eight neurodynamic and/or orthopedic tension tests using magnetic resonance imaging as the Gold Standard. Methods: An epidemiological study of randomized consecutive cases which was observational, descriptive, transversal, double blinded and was conducted following the Standards for Reporting Diagnostic accuracy studies (STARD) declaration. The sample size was 864 participants. Internal and external validity (CI = 95%) and reliability, were calculated for all tests performed independently. The diagnostic validity of the combined and multiple tests in parallel was also calculated. Results: The analysis indicated that only two tests performed independently had external validity, but neither had reliability or precision. The Straight Leg Raise test and the Bragard test performed in a multiple parallel way showed high sensitivity (97.40%), high negative predictive value (PV− 96.64%) and external validity (Likelihood Ratio− 0.05). The combined test of the Slump test and the Dejerine’s triad had internal and external validity. Conclusions: The Straight Leg Raise test and the Bragard test performed in a multiple parallel way and the combined test of the Slump Test and the Dejerine’s triad have clinical validity to discard lumbar or lumbar-sacral radiculopathy.
Sweet, Fat and Salty: Snacks in Vending Machines in Health and Social Care Institutions in Slovenia
Authors: Rozman Urška , Pravst Igor , Kupirovič Urška Pivk , Blaznik Urška , Kocbek Primož , Turk Sonja Šostar
Published: September 2020

Vending machines in health and social care facilities are often the only possible choice for a quick snack for workers and visitors, in many cases providing unhealthy dietary choices. Our study aimed to analyse the variety and nutritional quality of foods available in vending machines placed in social and health care institution in Slovenia. The available snacks were quantitatively assessed, using traffic light profiling. The model used for nutrient profiling was that of the Food Standards Australia New Zealand (FSANZ). Vending machines in 188 institutions were surveyed, resulting in 5625 food-items consisting of 267 unique product labels. Sweet products dominate in vending machines offers (about 70%), while nuts and seeds (8.4%), yoghurts (2.1%), fruits (1.4%) and milk (0.3%) are present in a very small proportion or are not available at all. According to FSANZ, 88.5% of all displayed food items in vending machines can be considered as lower nutritional quality or less healthy products. The authors’ future activities will be focused on ensuring wider availability of healthy dietary choices and on including official guidelines in tender conditions for vending machines in health and social care institutions in Slovenia.
Sarcopenia as a Mediator of the Effect of a Gerontogymnastics Program on Cardiorespiratory Fitness of Overweight and Obese Older Women: A Randomized Controlled Trial
Authors: Marcos-Pardo Pablo Jorge , González-Gálvez Noelia , Gea-García Gemma María , López-Vivancos Abraham , Espeso-García Alejandro , Gomes de Souza Vale Rodrigo
Published: September 2020

The objectives were to analyze the effect of a gerontogymnastics program on functional ability and fitness on overweight and obese older woman and to understand if sarcopenia mediates its effect. This randomized controlled trial involved 216 overweight and obese women. The experimental group (EG) carried out 12 weeks of a gerontogymnastics program. The assessment was of gait speed, cardiorespiratory fitness, functional capacity, and muscle strength. EG showed significant improvements in almost every test. When the effect of training was adjusted by gait speed, the improvement of the 6 min walk test (MWT) for the trained group was no longer significant (p = 0.127). The improvement of the 6 MWT was significantly and positively associated with the 10 m test (β = −10.087). After including the 10-m test in the equations, the association between the 6MWT and carrying out the training program decreased but remained significant (β = −19.904). The mediation analysis showed a significant, direct and indirect effect with a significant Sobel test value (z = 6.606 ± 7.733; p = 0.000). These results indicate that a gerontogymnastics program improves functional capacity and fitness; and the effect of a gerontogymnastics program on CRF is mediated by sarcopenia in older women who are overweight and obese.
Difference in Perception of Onset of Old Age in Traditional (Hadza) and Modern (Polish) Societies
Authors: Frąckowiak Tomasz , Groyecka-Bernard Agata , Oleszkiewicz Anna , Butovskaya Marina , Żelaźniewicz Agnieszka , Sorokowski Piotr
Published: September 2020

Despite relatively clear physiological indicators of old age, little is known about cross-cultural differences in psychological perceptions of the transition to old age. Although recent studies suggest consistency between modern countries, the subjective perception of old age onset in traditional societies remains poorly explored. Therefore, we compared the perception of timing of old age between a traditional tribe of hunter-gatherers (the Hadza) and a Polish sample representing a modern, industrialized population. The results indicate that the Hadza perceive old age onset as being significantly earlier than do the Poles. Furthermore, we found between-gender differences in the Polish sample: men set a lower threshold of old age onset than women. The Hadza showed no between-gender difference. Although the samples were matched for age, a larger proportion of Hadza considered themselves old. We discuss these findings from cultural and demographical perspectives.
Changes in Smoking and Vaping over 18 Months among Smokers and Recent Ex-Smokers: Longitudinal Findings from the 2016 and 2018 ITC Four Country Smoking and Vaping Surveys
Authors: Gravely Shannon , Meng Gang , Cummings K. Michael , Hyland Andrew , Borland Ron , Hammond David , O’Connor Richard J. , Goniewicz Maciej L. , Kasza Karin A. , McNeill Ann , Thompson Mary E. , Hitchman Sara C. , Levy David T. , Thrasher James F. , Quah Anne C.K. , Martin Nadia , Ouimet Janine , Boudreau Christian , Fong Geoffrey T.
Published: September 2020

This descriptive study of smokers (smoked at least monthly) and recent ex-smokers (quit for ≤2 years) examined transitions over an 18 month period in their smoking and vaping behaviors. Data are from Waves 1 (W1: 2016) and 2 (W2: 2018) of the ITC Four Country Smoking and Vaping Survey, a cohort study of adult (≥18+) smokers, concurrent users (smoke and vape), and recent ex-smokers from Australia, Canada, England, and the United States (US). Respondents (N = 5016) were classified according to their smoking and vaping status, which resulted in eight subgroups: (1) exclusive daily smokers (2) exclusive non-daily smokers; (3–6) concurrent users (subdivided into four groups by each combination of daily/non-daily smoking and daily/non-daily vaping); (7) ex-smokers who vape; (8) ex-smokers not vaping. The analyses focused first on describing changes between groups from W1 to W2. Second, transition outcomes were assessed based on changes in smoking and vaping between W1 and W2. Transitions focused on smoking were: no change in smoking (continued smoking at the same frequency); decreased smoking; increased smoking; discontinued smoking; relapsed (ex-smokers at W1 who were smoking at W2). Transitions focused on vaping were: initiated vaping; switched from smoking to vaping. Overall, this study found that the vast majority of smokers were smoking 18 months later. Non-daily smokers were more likely than daily smokers to have discontinued smoking (p < 0.0001) and to have switched to exclusive vaping (p = 0.034). Exclusive non-daily smokers were more likely than exclusive daily smokers to have initiated vaping (p = 0.04). Among all W1 daily smokers, there were no differences in discontinued smoking between daily smokers who vaped (concurrent users) and exclusive daily smokers; however, concurrent users were more likely than exclusive daily smokers to have decreased to non-daily smoking (p < 0.001) or to have switched to vaping by W2 (p < 0.001). Among all W1 non-daily smokers, there were no significant differences in increased smoking or discontinued smoking between concurrent users or exclusive smokers. Most ex-smokers remained abstinent from smoking, and there was no difference in relapse back to smoking between those who vaped and those who did not.
Early to Mid-Holocene Palaeoenvironment Change and Sedimentary Evolution in the Xianghu Area, Zhejiang
Authors: Sha Longbin , Li Xianfu , Tang Jiabing , Shu Junwu , Wang Weiming , Li Dongling
Published: September 2020

A 2.5 m long sediment core (XH-2) obtained from Xianghu area, near the Kuahuqiao site, were analyzed for grain size, diatom index, and geochemistry of organic carbon. The results of the total organic carbon (TOC) and stable organic carbon isotope (δ13C) in sediment samples from core XH-2 in the Xianghu area in Zhejiang Province have revealed the evolution history of sedimentary environmental and climatic changes during the breeding–prosperity–decline period of the Kuahuqiao culture. During 9300–8200 cal a BP, TOC contents were relatively high and stable, whereas δ13C values tended to be negative. This condition indicated that the climate was humid, and the sedimentary environment in the Xianghu area was stable. During 8200–7500 cal a BP, TOC contents presented a fluctuating declining trend, and δ13C values were significantly high, implying that the climate was arid, and the Xianghu area was gradually reduced to land. Thus, conducive conditions were provided for the development of the Kuohuqiao culture (7700–7400 cal a BP). From 7500 cal a BP, TOC contents obviously declined, and δ13C values were partially low, suggesting strengthened hydrodynamic force and wet conditions in the Xianghu area. This condition was related to the rise in sea level at approximately 7400 cal a BP, and the Kuahuqiao site became obsolete due to the transgression event. The TOC contents in core XH-2 were remarkably influenced by grain size, whereas no significant correlation existed between the δ13C variability and grain size. Sedimentary environment changes in the Xianghu area from 9300 to 6600 cal a BP, which was reflected by the TOC and δ13C records in core XH-2, accorded with the diatom results in this core and those in the Baima Lake area.
Dynamic Evaluation of Ecological Service Function Value of Qilihai Wetland in Tianjin
Authors: Chen Chen , Shao ChaoFeng , Shi YanMin
Published: September 2020

This study, using the method of economic quantitative analysis, studied the land use changes of Qilihai Wetland from 2008 to 2017, and the effects of these changes on the ES (ecosystem service) values of the wetland. This article benchmarked the 2030 Agenda for Sustainable Development, based on the systematic analysis and analysis of current ecological service function value evaluation methods. The research results show that the total values of the wetland ecosystem services in 2008, 2011, 2014, and 2017 were 317 million yuan, 299 million yuan, 283 million yuan, and 321 million yuan, respectively. In 2008 and 2011, the ES of the Wetland was mainly based on supply and support services, and in 2014 and 2017, it was mainly based on supply and regulation services. Changes in human utilization, natural conditions, and social economy will all lead to changes in the ES value of the whole Wetland. This research can enable decision makers to intuitively understand its ecological changes and plan the use of land and formulate ecological protection measures in a reasonable and effective manner. Finally, the article puts forward relevant suggestions for sustainable development based on the resource and environmental foundation and characteristics of Qilihai Wetland.
The Relationship between Individual-Level and Context-Level Factors and Social Distancing from Patients with Depression in Taiwan: A Multilevel Analysis of National Surveys
Authors: Tsai Chi-Hsuan , Kao Yu-Chen , Lien Yin-Ju
Published: September 2020

Background: Research on social distancing from patients with depression has primarily focused on individual-level factors rather than context-level factors. This study aimed to investigate the relationship between individual-level and context-level factors and social distancing from depressive patients. Methods: Sample data were collected via computer-assisted telephone interviews with 800 Taiwanese adults aged 20 to 65 years in 2016. All effects were tested using multilevel analysis. Results: With regard to individual-level variables, male sex, older age, people with more perceived dangerousness and those with more emotional reaction of fear were associated with greater social distancing from depressive patients. After controlling for individual-level variables, a positive association was found between the degree of urbanization and social distancing. We also found the interaction between the density of psychiatric rehabilitation services and perceived dangerousness to be associated with social distance. This finding revealed that persons with more perceived dangerousness and living in a region with higher density of psychiatric rehabilitation services were associated with greater social distance. Conclusions: We found that social distancing from depressive patients is not only determined by individual-level factors but influenced by the surroundings. This study provides useful directions for the implementation of optimal anti-stigma interventions for patients with depression.
Farmers’ Economic Status and Satisfaction with Homestead Withdrawal Policy: Expectation and Perceived Value
Authors: Zhao Yaoyang , Cloutier Scott , Li Hongqing
Published: September 2020

The withdrawal of homesteads is an effective way to improve the efficiency of rural construction land use and is being piloted in many regions of China, but the mechanism influencing farmers’ satisfaction with the withdrawal policy is unclear. This paper aimed to investigate the relationships among farmers’ economic status (ES), policy expectation (PE), policy perceived value (PPV), and farmers’ satisfaction with homestead withdrawal policy (policy satisfaction; PS). The study examined the mediating effects of PE and PPV on the relationship between ES and PS. The data obtained from a questionnaire of 287 households in Jinhu County, Jiangsu Province, China. After surviving from reliability and validity tests, a structural equation modeling (SEM) with latent variables was specified and estimated using Mplus. From the study results, we found significant positive relationships between ES, PP, and PS, but significant negative relationships between ES, PE, PPV, and PS. Also, our research found PE and PPV as potential mediators on ES-PS relation. We propose recommendations from three aspects to improve farmers’ satisfaction with future implementations of the homestead withdrawal policy. Our results provided new insights into how to improve the performance of homestead withdrawal policy.
The Spanish Version of the Internet Gaming Disorder Scale-Short Form (IGDS9-SF): Further Examination Using Item Response Theory
Authors: Maldonado-Murciano Laura , M. Pontes Halley , Griffiths Mark D. , Barrios Maite , Gómez-Benito Juana , Guilera Georgina
Published: September 2020

Internet gaming disorder (IGD) has been recognized by the American Psychiatric Association (APA) as a tentative disorder in the latest (fifth) revision of the Diagnostic and Statistical Manual of Mental Disorders (DSM-5). However, psychometric evaluation of the nine IGD criteria remains necessary to further enhance its assessment. Therefore, the objective of this study was to evaluate the psychometric properties of the Spanish version of the Internet Gaming Disorder Scale-Short Form (IGDS9-SF). The internal structure, internal consistency, temporal stability, and relationships with other variables were assessed. Furthermore, a polytomous item response theory (IRT) approach was used to evaluate the performance of each item and the test as a whole. A sample of 388 online gamers (53.61% women, mean age 25.45 years, standard deviation (SD) = 9.62) was recruited for this study. Similar to previous research, the results supported a one-factor structure for the IGDS9-SF, adequate internal consistency and temporal stability of scores, goodness of fit of the items to the graded response model (GRM), and more precise scores at high trait levels to assess IGD in Spanish populations. These findings corroborate the suitability of the Spanish IGDS9-SF for clinical assessment and research within Spanish-speaking populations.
Major and Trace Elements in Moldavian Orchard Soil and Fruits: Assessment of Anthropogenic Contamination
Authors: Zinicovscaia Inga , Sturza Rodica , Duliu Octavian , Grozdov Dmitrii , Gundorina Svetlana , Ghendov-Mosanu Aliona , Duca Gheorghe
Published: September 2020

The correct assessment of the presence of potentially contaminating elements in soil, as well as in fruits cultivated and harvested from the same places has major importance for both the environment and human health. To address this task, in the case of the Republic of Moldova where the fruit production has a significant contribution to the gross domestic product, the mass fractions of 37 elements (Na, Mg, Al, Ca, Si, K, Mn, Fe, Sc, Ti, V, Cr, Co, Ni, Zn, As, Br, Rb, Sr, Zr, Mo, Cd, Sb, Cs, Ba, La, Ce, Nd, Sm, Eu, Tb, Yb, Hf, Ta, W, Th, and U) were determined by instrumental neutron activation analysis in soil collected from four Moldavian orchards. In the case of three types of fruits, grapes, apples, and plums, all of them collected from the same places, only 22 elements (Na, Mg, Cl, K, Ca, Sc, Mn, Fe, Co, Ni, Cu, Zn, As, Br, Rb, Sr, Sb, Cs, Ba, La, Th, and U) were detected. The enrichment factor, contamination factor, geo-accumulation index, as well as pollution load index were calculated to assess the soil contamination. At the same time, the metal uptake from the soil into fruits was estimated by means of transfer factors. Soil samples showed for almost all elements mass fractions closer to the upper continental crust with the exception of a slightly increased content of As, Br, and Sb, but without overpassing the officially defined alarm thresholds. In the case of fruits, the hazard quotients for all elements with the exception of Sb in fruits collected in two orchards were below unity. A subsequent discriminant analysis allowed grouping all fruits according to their type and provenance.
Work-Related Psychosocial Demands and Resources in General Practice Teams in Germany. A Team-Based Ethnography
Authors: Tsarouha Elena , Preiser Christine , Weltermann Birgitta , Junne Florian , Seifried-Dübon Tanja , Stuber Felicitas , Hartmann Sigrid , Wittich Andrea , Rieger Monika A. , Rind Esther
Published: September 2020

General practices are established microenterprises in Germany providing a variety of preventive and therapeutic health care services and procedures in a challenging working environment. For example, general practice teams are confronted increasingly with work-related demands, which have been associated with poor psychological and physical outcomes. It is therefore important to gain a better understanding of issues related to occupational health and safety for personnel working in the primary care setting. This study aims to gain an in-depth understanding of psychosocial demands and resources in the primary care setting. We applied an ethnographic design, comprising a combination of participating observations, individual interviews with general practitioners (GPs) (N = 6), and focus group discussion with practice assistants and administrative staff (N = 19) in five general practices in Germany. A grounded theory approach was applied to analyze all data. Our results identified psychosocial demands and resources exemplified mainly along two typical tasks in GP practices: the issuing of medical prescriptions and blood sampling. Main psychosocial demands included factors related to work content and tasks, organization of work, and the working environment. For example, daily routines across all practices were characterized by a very high work intensity including disturbances, interruptions, delegation, and the division of labor between GPs and practice staff. Work-related resources comprised the staff’s influence on aspects related to work organization and social support. The triangulation of methods and data formats allowed the disclosure of interconnectedness between these factors. Although work processes in general practices are complex and required to comply with legal regulations, there are opportunities for practice owners and practice teams to establish working procedures in ways that reduce psychosocial risks and strengthen work-related resources.
The Feasibility and Preliminary Efficacy of an eHealth Lifestyle Program in Women with Recent Gestational Diabetes Mellitus: A Pilot Study
Authors: Rollo Megan E. , Baldwin Jennifer N. , Hutchesson Melinda , Aguiar Elroy J. , Wynne Katie , Young Ashley , Callister Robin , Haslam Rebecca , Collins Clare E.
Published: September 2020

Self-administered eHealth interventions provide a potential low-cost solution for reducing diabetes risk. The aim of this pilot randomised controlled trial (RCT) was to evaluate the feasibility, including recruitment, retention, preliminary efficacy (primary outcome) and acceptability (secondary outcome) of the “Body Balance Beyond” eHealth intervention in women with previous gestational diabetes mellitus (GDM). Women with overweight/obesity who had recent GDM (previous 24 months) were randomised into one of three groups: 1) high personalisation (access to “Body Balance Beyond” website, individual telehealth coaching via video call by a dietitian and exercise physiologist, and text message support); 2) low personalisation (website only); or 3) waitlist control. To evaluate preliminary efficacy, weight (kg), glycosylated hemoglobin, type A1C (HbA1c), cholesterol (total, low-density lipoprotein (LDL) and high-density lipoprotein (HDL)), diet quality and moderate–vigorous physical activity were analysed at baseline and at 3 and 6 months using generalised linear mixed models. To investigate acceptability, process evaluation was conducted at 3 and 6 months. Of the 327 potential participants screened, 42 women (mean age 33.5 ± 4.0 years and BMI 32.4 ± 4.3 kg/m2) were randomised, with 30 (71%) completing the study. Retention at 6 months was 80%, 54% and 79% for high personalisation, low personalisation and waitlist control, respectively (reasons: personal/work commitments, n = 4; started weight-loss diet, n = 1; pregnant, n = 1; resources not useful, n = 1; and not contactable, n = 5). No significant group-by-time interactions were observed for preliminary efficacy outcomes, with the exception of HDL cholesterol, where a difference favoured the low personalisation group relative to the control (p = 0.028). The majority (91%) of women accessed the website in the first 3 months and 57% from 4–6 months. The website provided useful information for 95% and 92% of women at 3 and 6 months, respectively, although only a third of women found it motivating (30% and 25% at 3 and 6 months, respectively). Most women agreed that the telehealth coaching increased their confidence for improving diet (85%) and physical activity (92%) behaviours, although fewer women regarded the text messages as positive (22% and 31% for improving diet and physical activity, respectively). The majority of women (82% at 3 months and 87% at 6 months) in the high personalisation group would recommend the program to other women with GDM. Recruiting and retaining women with a recent diagnosis of GDM is challenging. The “Body Balance Beyond” website combined with telehealth coaching via video call is largely acceptable and useful for women with recent GDM. Further analysis of the effect on diabetes risk reduction in a larger study is needed.
Site Selection for Pre-Hospital Emergency Stations Based on the Actual Spatiotemporal Demand: A Case Study of Nanjing City, China
Authors: Han Bing , Hu Mingxing , Wang Jialing
Published: September 2020

Rapid economic and social development has been accompanied by the occurrence of many major issues throughout the world. Specifically, there is an ever-increasing demand for emergent medical services among the public. In order to ensure timely responses to emergency demands, it is critical to reasonably configure the emergency stations. In general, emergency stations are mostly distributed according to the distribution of emergency demands and response time, which, however, fails to consider the negative impacts of randomly occurring emergency demands and traffic delays. In this study, first aid demands are combined with traffic states based on the spatiotemporal big data set covering model, which alleviates the negative impacts of randomly occurring first aid demands and traffic delay time on the planning of pre-hospital first aid stations. Moreover, the accuracy of the site selection model is improved, which meets the requirements that all randomly occurring simulated first aid demands can be approached within the target time under planning conditions and actual traffic constraints. Taking Nanjing City as an example, this study obtains multi-source big data, such as ambulance-carried GPS data from June 2018 to June 2019, Gaode Map-recorded traffic congestion data, and survey data of emergency rescue facilities. Basing on the processing and analysis of these data, it shows that first aid demands in Nanjing City are highly region-specific with high time delay. Various required factors are determined based on modeling and analysis, and the target time is agreed to be 8 min. The average vehicle speed on each road is calculated, accompanied by the establishment of an actual road network model. In this context, the transit time from the randomly distributed first aid stations to the hospital can be calculated, which are set to satisfy the model conditions so as to obtain the solution. Finally, such a solution is adjusted and verified according to the land-use situation. The results of this study, based on spatiotemporal big data, are expected to provide insights into improving the site selection model and enhancing efficiency while providing new technical methods.
Efficient Delivery and Replication of Oncolytic Virus for Successful Treatment of Head and Neck Cancer
Authors: Hamada Masakazu , Yura Yoshiaki
Published: September 2020

Head and neck cancer has been treated by a combination of surgery, radiation, and chemotherapy. In recent years, the development of immune checkpoint inhibitors (ICIs) has made immunotherapy a new treatment method. Oncolytic virus (OV) therapy selectively infects tumor cells with a low-pathogenic virus, lyses tumor cells by the cytopathic effects of the virus, and induces anti-tumor immunity to destroy tumors by the action of immune cells. In OV therapy for head and neck squamous cell carcinoma (HNSCC), viruses, such as herpes simplex virus type 1 (HSV-1), vaccinia virus, adenovirus, reovirus, measles virus, and vesicular stomatitis virus (VSV), are mainly used. As the combined use of mutant HSV-1 and ICI was successful for the treatment of melanoma, studies are underway to combine OV therapy with radiation, chemotherapy, and other types of immunotherapy. In such therapy, it is important for the virus to selectively replicate in tumor cells, and to express the viral gene and the introduced foreign gene in the tumor cells. In OV therapy for HNSCC, it may be useful to combine systemic and local treatments that improve the delivery and replication of the inoculated oncolytic virus in the tumor cells.
Development of Heterocycle-Substituted and Fused Azulenes in the Last Decade (2010–2020)
Authors: Shoji Taku , Okujima Tetsuo , Ito Shunji
Published: September 2020

Azulene derivatives with heterocyclic moieties in the molecule have been synthesized for applications in materials science by taking advantage of their unique properties. These derivatives have been prepared by various methods, involving electrophilic substitution, condensation, cyclization, and transition metal-catalyzed cross-coupling reactions. Herein, we present the development of the synthetic methods, reactivities, and physical properties for the heterocycle-substituted and heterocycle-fused azulenes reported in the last decade.
Chaperone Sigma1R and Antidepressant Effect
Authors: Voronin Mikhail V. , Vakhitova Yulia V. , Seredenin Sergei B.
Published: September 2020

This review analyzes the current scientific literature on the role of the Sigma1R chaperone in the pathogenesis of depressive disorders and pharmacodynamics of antidepressants. As a result of ligand activation, Sigma1R is capable of intracellular translocation from the endoplasmic reticulum (ER) into the region of nuclear and cellular membranes, where it interacts with resident proteins. This unique property of Sigma1R provides regulation of various receptors, ion channels, enzymes, and transcriptional factors. The current review demonstrates the contribution of the Sigma1R chaperone to the regulation of molecular mechanisms involved in the antidepressant effect.
MOS1 Negatively Regulates Sugar Responses and Anthocyanin Biosynthesis in Arabidopsis
Authors: Zhang Ning , Wang Maike , Huang Jie , Yang Leiyun , Wang Zhixue , Wu Dianxing , Shu Xiaoli
Published: September 2020

Sugars, which are important signaling molecules, regulate diverse biological processes in plants. However, the convergent regulatory mechanisms governing these physiological activities have not been fully elucidated. MODIFIER OF snc1-1 (MOS1), a modulator of plant immunity, also regulates floral transition, cell cycle control, and other biological processes. However, there was no evidence of whether this protein was involved in sugar responses. In this study, we found that the loss-of-function mutant mos1-6 (mos1) was hypersensitive to sugar and was characterized by defective germination and shortened roots when grown on high-sugar medium. The expression of MOS1 was enhanced by sucrose. Hexokinase 1, an important gene involved in sugar signaling, was upregulated in the mos1 mutant compared to wild-type Col-0 in response to sugar. Furthermore, the mos1 mutant accumulated more anthocyanin than did wild-type Col-0 when grown on high-sugar concentration medium or under high light. MOS1 was found to regulate the expression of flavonoid and anthocyanin biosynthetic genes in response to exogenous sucrose and high-light stress but with different underlying mechanisms, showing multiple functions in addition to immunity regulation in plant development. Our results suggest that the immune regulator MOS1 serves as a coordinator in the regulatory network, governing immunity and other physiological processes.