bluuebunny/crossref_metadata_2025 huggingface dataset:
Enhancing antibacterial efficacy and accelerating infectious wound healing in rats using biogenic metal nanoparticles from marine Bacillus subtilis
Authors: Pemmadi Raghuveer Varma , Alhakamy Nabil Abdulhafiz , Asfour Hani Z. , Kotta Sabna , Alfaleh Mohamed A. , Sunnapu Prasad , Uk Ilyas , Pottail Lalitha , Chithambharan Akhila , Yogananthan Dhanapal , Thavamani B Samuel , Valiyaparambil Shilpa , Kallungal Sirajudheen Mukriyan , Kotakonda Muddukrishnaiah
Published: February 2024

Introduction

Microorganisms originating from the marine environment, such as bacteria, fungi, and algae, are deliberately employed in the production of nanoparticles on account of the wide array of bioactive compounds they produce.

Methods

Cell-free aqueous extracts of marine Bacillus subtilis (CBPPR1) were used to synthesise AuNPs (CBPPR1AuNPs) and AgNPs (CBPPR1AgNPs). Zetasizer Nano ZS (Malvern Instruments) zeta size and zeta potential, field emission and transmission scanning electron microscopy (FE-SEM and HR-TEM), UV-visible (UV-Vis), X-ray diffraction (XRD), Fourier transform infrared (FT-IR), and EDAX were used to characterize biogenically synthesized nanoparticles (NPs). Their antibacterial activities against Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus were investigated. The anticancer efficacy of CBPPR1Au and AgNPs was investigated in human colorectal adenocarcinoma cell lines (HT-29, HT-116). CBPPR1AgNPs formulation was studied in vitro and in-vivo rat models. The assessment focused on its efficacy in wound healing and antibacterial capabilities, comparing them against a commercial product. To determine the effectiveness of CBPPR1AgNPs in wound healing, a cutaneous wound model was employed, which included infection with S. aureus.

Results and discusion

CBPPR1Au and AgNPs significantly inhibited the growth of S aureus at MIC of 125 µg (CBPPR1AuNPs) and 62.5 µg (CBPPR1AgNPs) respectively. FE-SEM and HR-TEM observations confirmed that NPs caused bacterial cell leakage, damage, and shrinkage. Cancer cell viability was reduced upon treatment with increasing concentrations of CBPPR1Au and AgNPs, and apoptosis was increased in cells treated with CBPPR1Au and AgNPs relative to untreated cells (p < 0.001). CBPPR1Au and AgNPs showed significant cytotoxic activity against HT-29 (15.5 M) and HT-116 (62.5 M) cells. In-vivo experiments on rats showed minimal pus formation in groups CBPPR1AgNPs (62.5 µg/ml) G2, CBPPR1AgNPs (125 µg/ml) G3, and silver sulfadiazine G4, indicating the effective control of infections. CBPPR1AgNPs-treated wounds showed complete closure, whereas untreated G1 wounds remained unhealed. Histopathological analysis showed no adverse effects of CBPPR1AgNPs on kidneys and livers of rats. These findings suggest that CBPPR1AgNPs play a pivotal role in wound healing because of their potent antibacterial properties.
18F-FDG PET/CT assists the diagnosis of primary pancreatic lymphoma: Two case reports and literature review
Authors: Wang Jian , Zhou Yujing , Liu Hongwei , Zhou Jianli , Li Xin
Published: February 2024

Primary pancreatic lymphoma (PPL) is a rare malignancy, which is defined as a mass centered in pancreas with involvement of contiguous lymph nodes and distant spread may exist. Accurate diagnosis of PPL prior to pathological confirmation remains challenging, underscoring the critical significance of preoperative imaging assessments. This case report collected two instances of PPL that underwent initial evaluation via 18F-fluorodeoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT) between August 2021 and July 2022. Correspondingly, pertinent literature encompassing 18F-FDG PET/CT data related to PPL was meticulously reviewed. Including our aforementioned pair of cases, a cumulative total of 25 instances of PPL were assembled. The distinctive profile of 18F-FDG PET/CT images of PPL predominantly manifests as hypermetabolic lesions with diminished density. Primarily characterized by singular lesions and comparatively substantial volumetric dimensions, a total of eleven cases revealed contiguous lymph node engagement, with five instances displaying distant dissemination encompassing lymph nodes in multiple locations. Amongst these, ten patients underwent sequential 18F-FDG PET/CT follow-up post-intervention. In comparison to pancreatic carcinoma, PPL lesions exhibited heightened hypermetabolism, augmented volumetric proportions, and distinct patterns of distant metastasis. This study indicates that the pivotal role of 18F-FDG PET/CT in the diagnosis and assessment of therapeutic efficacy in PPL is unequivocal. Combined with the clinical attributes of patients, the integration of 18F-FDG PET/CT augments the differential diagnostic capacity differentiating PPL from pancreatic carcinoma.
Interactions between Ras and Rap signaling pathways during neurodevelopment in health and disease
Authors: Cherra Salvatore J. , Lamb Reagan
Published: February 2024

The Ras family of small GTPases coordinates tissue development by modulating cell proliferation, cell-cell adhesion, and cellular morphology. Perturbations of any of these key steps alter nervous system development and are associated with neurological disorders. While the underlying causes are not known, genetic mutations in Ras and Rap GTPase signaling pathways have been identified in numerous neurodevelopmental disorders, including autism spectrum, neurofibromatosis, intellectual disability, epilepsy, and schizophrenia. Despite diverse clinical presentations, intersections between these two signaling pathways may provide a better understanding of how deviations in neurodevelopment give rise to neurological disorders. In this review, we focus on presynaptic and postsynaptic functions of Ras and Rap GTPases. We highlight various roles of these small GTPases during synapse formation and plasticity. Based on genomic analyses, we discuss how disease-related mutations in Ras and Rap signaling proteins may underlie human disorders. Finally, we discuss how recent observations have identified molecular interactions between these pathways and how these findings may provide insights into the mechanisms that underlie neurodevelopmental disorders.
Radiomics analysis of R2* maps to predict early recurrence of single hepatocellular carcinoma after hepatectomy
Authors: Li Jia , Ma Yunhui , Yang Chunyu , Qiu Ganbin , Chen Jingmu , Tan Xiaoliang , Zhao Yue
Published: February 2024

Objectives

This study aimed to evaluate the effectiveness of radiomics analysis with R2* maps in predicting early recurrence (ER) in single hepatocellular carcinoma (HCC) following partial hepatectomy.

Methods

We conducted a retrospective analysis involving 202 patients with surgically confirmed single HCC having undergone preoperative magnetic resonance imaging between 2018 and 2021 at two different institutions. 126 patients from Institution 1 were assigned to the training set, and 76 patients from Institution 2 were assigned to the validation set. A least absolute shrinkage and selection operator (LASSO) regularization was conducted to operate a logistic regression, then features were identified to construct a radiomic score (Rad-score). Uni- and multi-variable tests were used to assess the correlations of clinicopathological features and Rad-score with ER. We then established a combined model encompassing the optimal Rad-score and clinical-pathological risk factors. Additionally, we formulated and validated a predictive nomogram for predicting ER in HCC. The nomogram’s discrimination, calibration, and clinical utility were thoroughly evaluated.

Results

Multivariable logistic regression revealed the Rad-score, microvascular invasion (MVI), and α fetoprotein (AFP) level > 400 ng/mL as significant independent predictors of ER in HCC. We constructed a nomogram based on these significant factors. The areas under the receiver operator characteristic curve of the nomogram and precision-recall curve were 0.901 and 0.753, respectively, with an F1 score of 0.831 in the training set. These values in the validation set were 0.827, 0.659, and 0.808.

Conclusion

The nomogram that integrates the radiomic score, MVI, and AFP demonstrates high predictive efficacy for estimating the risk of ER in HCC. It facilitates personalized risk classification and therapeutic decision-making for HCC patients.
Case report: Pathological complete response of pregnancy associated pulmonary enteric adenocarcinoma to chemoradiotherapy
Authors: Nemoto Yukiko , Kuroda Koji , Oyama Rintaro , Mori Masataka , Shimajiri Shohei , Tanaka Fumihiro
Published: February 2024

Pulmonary enteric adenocarcinoma (PEAC) is a rare lung adenocarcinoma with morphological features similar to those of primary and metastatic colorectal adenocarcinoma. To date, only a few studies have reported the therapeutic effects of chemoradiotherapy on PEAC. This report describes the case of a 28-year-old woman with pregnancy-related PEAC who presented with left shoulder pain. A superior sulcus tumor was identified in the left thoracic cavity, and the biopsy indicated more than 50% intestinal differentiation components. Moreover, immunohistochemical staining revealed positive CDX2 and CK7 expression. Positron emission tomography-computed tomography, upper endoscopy, colonoscopy, and small intestinal capsule endoscopy revealed no gastrointestinal malignancies. The patient was diagnosed with locally advanced PEAC (clinical stage T4N0M0; stage IIIA). Therefore, the patient was treated with preoperative chemoradiotherapy and underwent gross total resection during surgery. Pathological evaluation of the specimen revealed no residual tumor, indicating that the chemoradiotherapy for PEAC was highly effective. One subsequent brain metastasis was also resected, and the patient has not experienced recurrence in 28 months since this resection and continues to be monitored regularly. This is the first pathologically confirmed report of the use of chemoradiotherapy (carboplatin [CBDCA] and paclitaxel [PTX]) for PEAC and its clinical efficacy. Unlike previous reports, the efficacy of this treatment is attributed to the use of PTX in preoperative chemotherapy and the p21− status of the patient, which may have increased sensitivity to chemoradiation therapy. Therefore, chemoradiotherapy (CBDCA + PTX) may be a viable treatment option for advanced intestinal lung adenocarcinoma.
Overexpression of circulating CD38+ NK cells in colorectal cancer was associated with lymph node metastasis and poor prognosis
Authors: Wang Xueling , Li Haoran , Chen Huixian , Fang Kehua , Chang Xiaotian
Published: February 2024

Introduction

Lymph node metastasis (LNM) is a critical prognostic factor for colorectal cancer (CRC). Due to the potential influence of immune system on CRC progression, investigation into lymphocyte subsets as clinical markers has gained attention. The objective of this study was to assess the capability of lymphocyte subsets in evaluating the lymph node status and prognosis of CRC.

Methods

Lymphocyte subsets, including T cells (CD3+), natural killer cells (NK, CD3- CD56+), natural killer-like T cells (NK-like T, CD3+ CD56+), CD38+ NK cells (CD3- CD56+ CD38+) and CD38+ NK-like T cells (CD3+ CD56+ CD38+), were detected by flow cytometry. Univariate and multivariate analyses were used to assess the risk factors of LNM. The prognostic role of parameters was evaluated by survival analysis.

Results

The proportion of CD38+ NK cells within the NK cell population was significantly higher in LNM-positive patients (p <0.0001). However, no significant differences were observed in the proportions of other lymphocyte subsets. Poorer histologic grade (odds ratio [OR] =4.76, p =0.03), lymphovascular invasion (LVI) (OR =22.38, p <0.01), and CD38+ NK cells (high) (OR =4.54, p <0.01) were identified as independent risk factors for LNM. Furthermore, high proportion of CD38+ NK cells was associated with poor prognosis of CRC patients (HR=2.37, p =0.03).

Conclusions

It was demonstrated that the proportion of CD38+ NK cells was a marker overexpressed in LNM-positive patients compared with LNM-negative patients. Moreover, an elevated proportion of CD38+ NK cells is a risk factor for LNM and poor prognosis in CRC.
Access to advanced healthcare services and its associated factors among patients with cervical cancer in Addis Ababa, Ethiopia
Authors: Shimels Tariku , Gashawbeza Biruck , Gedif Fenta Teferi
Published: February 2024

Objective

This study aimed to assess the extent of access to healthcare services, perception and associated factors among patients with cervical cancer in Addis Ababa, Ethiopia.

Methods

A facility-based cross-sectional study was conducted from 01 July through 30 August 2023 at two tertiary hospitals. Cases with histopathologic and clinical confirmation of cervical cancer were enrolled using a consecutive sampling technique. Data was collected through a validated questionnaire administered by interviewers using the KoboCollect application. Subsequently, the collected data underwent analysis using Statistical Sciences for Social Sciences (SPSS) version 26.0. Bivariable and multivariable regression models were performed at p ≤ 0.2 and p<0.05 statistical significance, respectively.

Results

A total of 391 patients were enrolled in the study. Health facilities were accessible for obtaining general medical services (56.5%), drugs (57.3%), laboratory diagnosis services (57.0%), imaging diagnosis services (56.8%), and radiotherapy services (55.8%) of the patients. Cost of services was affordable only in 11.5% of the cases. Essential anticancer medicines were out of stock for 229 (58.6%) of the patients during the past three months. About two-thirds of the patients have a good perception of access to healthcare services. In multivariable binary logistic regression, admission to the inpatient (AOR: 0.20, 95% CI: 0.06-0.67), joblessness (AOR: 0.19, 95% CI: 0.08-0.46), lower level of income to the extreme poverty line (3567ETB)(64.9 USD) (AOR: 0.19, 95% CI: 0.10-0.35), no CBHI coverage (AOR: 4.16, 95% CI: 1.76-9.85), having social support (AOR: 3.80; 95% CI: 1.96-7.41), and poor dietary practice (AOR: 2.36, 95% CI: 1.28-4.35) were found to have a statistically significant association with perceived good access to healthcare services.

Conclusion

Only close to a half of the patients with cervical cancer, in Addis Ababa, have adequate access to healthcare services. Nearly two-thirds of the patients reported perceived good access to the services. Many factors were found to show a statistically significant association with patients’ perceived access to healthcare services.
Unveiling the potential of Butylphthalide: inhibiting osteoclastogenesis and preventing bone loss
Authors: Yanbin Feng , Yilin Teng , Yaomin Mo , Deshuang Xi , Junhong Zhou , Gaofeng Zeng , Shaohui Zong
Published: February 2024

Osteoporosis, resulting from overactive osteoclasts and leading to elevated fracture risk, has emerged as a global public health concern due to the aging population. Therefore, inhibiting osteoclastogenesis and bone resorption function represents a crucial approach for preventing and treating osteoporosis. The purpose of this study was to examine the effects and molecular mechanisms of Butylphthalide (NBP) on the differentiation and function of osteoclasts induced by RANKL. Osteoclastogenesis was assessed through TRAP staining and bone slice assay. An animal model that underwent ovariectomy, simulating postmenopausal women’s physiological characteristics, was established to investigate the impact of Butylphthalide on ovariectomy-induced bone loss. To delve deeper into the specific mechanisms, we employed Western blot, PCR, immunofluorescence, and immunohistochemical staining to detect the expression of proteins that are associated with the osteoclast signaling pathway. In this study, we found that Butylphthalide not only suppressed osteoclastogenesis and bone resorption in vitro but also significantly decreased TRAcP-positive osteoclasts and prevented bone loss in vivo. Further mechanistic experiments revealed that Butylphthalide reduces intracellular ROS in osteoclasts, inhibits the MAPK and NFATc1 signaling pathways, and downregulates the key genes and proteins of osteoclasts. This inhibits osteoclast formation and function. The reduction in ROS in osteoclasts is intricately linked to the activity of Butylphthalide-modulated antioxidant enzymes. Overall, NBP may offer a alternative treatment option with fewer side effects for skeletal diseases such as osteoporosis.
Quantification of stimulus-evoked tactile allodynia in free moving mice by the chainmail sensitivity test
Authors: Ozdemir Yildirim , Nakamoto Kazuo , Boivin Bruno , Bullock Daniel , Andrews Nick A. , González-Cano Rafael , Costigan Michael
Published: February 2024

Chronic pain occurs at epidemic levels throughout the population. Hypersensitivity to touch, is a cardinal symptom of chronic pain. Despite dedicated research for over a century, quantifying this hypersensitivity has remained impossible at scale. To address these issues, we developed the Chainmail Sensitivity Test (CST). Our results show that control mice spend significantly more time on the chainmail portion of the device than mice subject to neuropathy. Treatment with gabapentin abolishes this difference. CST-derived data correlate well with von Frey measurements and quantify hypersensitivity due to inflammation. Our study demonstrates the potential of the CST as a standardized tool for assessing mechanical hypersensitivity in mice with minimal operator input.
Comparative quantitative trait loci analysis framework reveals relationships between salt stress responsive phenotypes and pathways
Authors: Phosuwan Sunadda , Nounjan Noppawan , Theerakulpisut Piyada , Siangliw Meechai , Charoensawan Varodom
Published: February 2024

Soil salinity is a complex abiotic stress that involves several biological pathways. Hence, focusing on a specific or a few salt-tolerant phenotypes is unlikely to provide comprehensive insights into the intricate and interwinding mechanisms that regulate salt responsiveness. In this study, we develop a heuristic framework for systematically integrating and comprehensively evaluating quantitative trait loci (QTL) analyses from multiple stress-related traits obtained by different studies. Making use of a combined set of 46 salinity-related traits from three independent studies that were based on the same chromosome segment substitution line (CSSL) population of rice (Oryza sativa), we demonstrate how our approach can address technical biases and limitations from different QTL studies and calling methods. This allows us to compile a comprehensive list of trait-specific and multi-trait QTLs, as well as salinity-related candidate genes. In doing so, we discover several novel relationships between traits that demonstrate similar trends of phenotype scores across the CSSLs, as well as the similarities between genomic locations that the traits were mapped to. Finally, we experimentally validate our findings by expression analyses and functional validations of several selected candidate genes from multiple pathways in rice and Arabidopsis orthologous genes, including OsKS7 (ENT-KAURENE SYNTHASE 7), OsNUC1 (NUCLEOLIN 1) and OsFRO1 (FERRIC REDUCTASE OXIDASE 1) to name a few. This work not only introduces a novel approach for conducting comparative analyses of multiple QTLs, but also provides a list of candidate genes and testable hypotheses for salinity-related mechanisms across several biological pathways.
Real-time and lightweight detection of grape diseases based on Fusion Transformer YOLO
Authors: Liu Yifan , Yu Qiudong , Geng Shuze
Published: February 2024

Introduction

Grapes are prone to various diseases throughout their growth cycle, and the failure to promptly control these diseases can result in reduced production and even complete crop failure. Therefore, effective disease control is essential for maximizing grape yield. Accurate disease identification plays a crucial role in this process. In this paper, we proposed a real-time and lightweight detection model called Fusion Transformer YOLO for 4 grape diseases detection. The primary source of the dataset comprises RGB images acquired from plantations situated in North China.

Methods

Firstly, we introduce a lightweight high-performance VoVNet, which utilizes ghost convolutions and learnable downsampling layer. This backbone is further improved by integrating effective squeeze and excitation blocks and residual connections to the OSA module. These enhancements contribute to improved detection accuracy while maintaining a lightweight network. Secondly, an improved dual-flow PAN+FPN structure with Real-time Transformer is adopted in the neck component, by incorporating 2D position embedding and a single-scale Transformer Encoder into the last feature map. This modification enables real-time performance and improved accuracy in detecting small targets. Finally, we adopt the Decoupled Head based on the improved Task Aligned Predictor in the head component, which balances accuracy and speed.

Results

Experimental results demonstrate that FTR-YOLO achieves the high performance across various evaluation metrics, with a mean Average Precision (mAP) of 90.67%, a Frames Per Second (FPS) of 44, and a parameter size of 24.5M.

Conclusion

The FTR-YOLO presented in this paper provides a real-time and lightweight solution for the detection of grape diseases. This model effectively assists farmers in detecting grape diseases.
Efficacy of music therapy on stress and anxiety prior to dental treatment: a systematic review and meta-analysis of randomized clinical trials
Authors: López-Valverde Nansi , López-Valverde Antonio , Macedo de Sousa Bruno , Blanco Rueda José Antonio
Published: February 2024

Introduction

Stress and anxiety are emotional states that often accompany patients who have to receive dental treatments, leading them to postpone or avoid treatments with the consequent deterioration of their oral health and, hence, their general condition. Music therapy has been shown to be an alternative to other treatments that are invasive and not without danger, such as anxiolytics or sedation. This systematic review and meta-analysis evaluated the effect of music therapy on anxiety and stress prior to dental treatments.

Methods

Studies published in PubMed (through Medline), Web of Science (WOS), Embase, and Cochrane Library databases were consulted up to October 2023. The inclusion criteria were established for intervention studies (randomized controlled trials, RCTs) according to the PICOS (population, intervention, comparison, outcomes, and study) strategy in subjects with dental stress and anxiety (participants) treated with music therapy (intervention) in comparison with patients without music therapy (control) and evaluating the response to treatment (outcomes).

Results

A total of 154 results were obtained, with 14 studies finally selected. The risk of bias and the methodological quality were assessed using the Cochrane Risk of Bias Tool and the Jadad scale, respectively. A random-effects meta-analysis was used to quantify the results of the pooled studies, while a fixed-effects meta-analysis was used for studies in the pediatric population. The meta-analysis of pooled studies found statistical significance in the subgroups of anxiety and anxiety–stress (p = 0.03 and p = 0.05, respectively), with an overall effect in favor of the intervention group (p = 0.005). Meta-analysis of the studies in the pediatric population showed considerable statistical significance for the experimental group (p < 0.00001).

Conclusion

Music therapy as a treatment for stress and anxiety, prior to dental treatment, proved to be effective in both children and adults although more well-designed randomized clinical studies are needed to validate its efficacy.

Systematic review registration

INPLASY, identifier 202312000.
Return to work, work productivity loss and activity impairment in Chinese breast cancer survivors 12-month post-surgery: a longitudinal study
Authors: Ng Danielle Wing Lam , So Serana Chun Yee , Fielding Richard , Mehnert-Theuerkauf Anja , Kwong Ava , Suen Dacita , Wong Ling , Fung Sara Wai Wun , Chun Oi Kwan , Fong Daniel Y. T. , Chan Sharon , Molasiotis Alex , So Winnie K. W. , Lam Wendy Wing Tak
Published: February 2024

Introduction

Existing evidence of returning-to-work (RTW) after cancer comes predominately from Western settings, with none prospectively examined since the initial diagnostic phase. This study prospectively documents RTW-rate, time-to-RTW, work productivity loss, and activity impairment, within the first-year post-surgery among Chinese women with breast cancer (BCW) and identify potential causal co-variants.

Methods

This observational longitudinal study followed 371 Chinese BCW who were employed/self-employed at the time of diagnosis at 4-week post-surgery (baseline). RTW-status and time-to-RTW were assessed at baseline (T1), 4-month (T2), 6-month (T3), and 12-month (T4) post-baseline. WPAI work productivity loss and activity impairment were assessed at T4. Baseline covariates included demographics, medical-related factors, work satisfaction, perceived work demand, work condition, RTW self-efficacy, B-IPQ illness perception, COST financial well-being, EORTC QLQ-C30 and QLQ-BR23 physical and psychosocial functioning, and HADS psychological distress.

Results

A 68.2% RTW-rate (at 12-month post-surgery), prolonged delay in RTW (median = 183 days), and significant proportions of T4 work productivity loss (20%), and activity impairment (26%), were seen. BCW who were blue-collar workers with lower household income, poorer financial well-being, lower RTW self-efficacy, poorer job satisfaction, poorer illness perception, greater physical symptom distress, impaired physical functioning, and unfavorable work conditions were more likely to experience undesired work-related outcomes.

Discussion

Using a multifactorial approach, effective RTW interventions should focus on not only symptom management, but also to address psychosocial and work-environmental concerns. An organizational or policy level intervention involving a multidisciplinary team comprising nurses, psychologists, occupational health professionals, and relevant stakeholders in the workplace might be helpful in developing a tailored organizational policy promoting work-related outcomes in BCW.
Symmetry of a partially-ligated state maintained by dynamics in a negatively cooperative system
Authors: Strawn Rebecca , Murthy Parvathi S. , Ettrich Rüdiger H. , Pelczer István , Carey Jannette
Published: February 2024

Symmetry was a key concept underlying the MWC model for allostery advanced in 1965 by Monod, Wyman, and Changeux. The reciprocal interactions of symmetrically-arranged identical subunits were proposed to stabilize multimeric assemblies together with the free energy from bound ligands that progressively favor a monomer-like state. Structural symmetry of subunits was assumed to be maintained in the partially-ligated states, even if ligand placement itself is not symmetric. Partially-ligated states can be populated sufficiently for experimental study only in negatively cooperative systems, which were not considered in the MWC model. The work reported here uses 1H, 13C, 15N, and 19F NMR to evaluate the structural symmetry of the hexameric arginine repressor of E. coli, a negatively cooperative system, with a single bound L-arginine ligand. The analysis indicates that the singly-ligated hexamer maintains structural symmetry as probed by these four NMR nuclei. The results are consistent with earlier molecular dynamics simulations suggesting that the global dynamics of the singly-ligated assembly are harnessed to maintain structural symmetry. The results extend MWC symmetry concepts to this negatively cooperative system, and indicate a role for global dynamics in allostery.
Development of a Cognitive Training Support Programme for prevention of dementia and cognitive decline in at-risk older adults
Authors: de Jager Loots Celeste A. , Price Geraint , Barbera Mariagnese , Neely Anna Stigsdotter , Gavelin Hanna M. , Lehtisalo Jenni , Ngandu Tiia , Solomon Alina , Mangialasche Francesca , Kivipelto Miia
Published: February 2024

Background

Evidence for the beneficial effects of cognitive training on cognitive function and daily living activities is inconclusive. Variable study quality and design does not allow for robust comparisons/meta-analyses of different cognitive training programmes. Fairly low adherence to extended cognitive training interventions in clinical trials has been reported.

Aims

The aim of further developing a Cognitive Training Support Programme (CTSP) is to supplement the Computerised Cognitive Training (CCT) intervention component of the multimodal Finnish Geriatric Intervention Study to Prevent Cognitive Impairment and Disability (FINGER), which is adapted to different cultural, regional and economic settings within the Word-Wide FINGERS (WW-FINGERS) Network. The main objectives are to improve adherence to cognitive training through a behaviour change framework and provide information about cognitive stimulation, social engagement and lifestyle risk factors for dementia.

Methods

Six CTSP sessions were re-designed covering topics including (1) CCT instructions and tasks, (2) Cognitive domains: episodic memory, executive function and processing speed, (3) Successful ageing and compensatory strategies, (4) Cognitive stimulation and engagement, (5) Wellbeing factors affecting cognition (e.g., sleep and mood), (6) Sensory factors. Session content will be related to everyday life, with participant reflection and behaviour change techniques incorporated, e.g., strategies, goal-setting, active planning to enhance motivation, and adherence to the CCT and in relevant lifestyle changes.

Conclusions

Through interactive presentations promoting brain health, the programme provides for personal reflection that may enhance capability, opportunity and motivation for behaviour change. This will support adherence to the CCT within multidomain intervention trials. Efficacy of the programme will be evaluated through participant feedback and adherence metrics.
Understanding public support for workplace diversity and antidiscrimination policies in Europe
Authors: Blommaert Lieselotte , Coenders Marcel
Published: February 2024

Societal processes and public opinion can affect whether employers take action and which policy measures they choose to boost diversity, equal opportunities and inclusion, and to reduce discrimination in the workplace. Yet, public opinion regarding workplace diversity initiatives (other than affirmative action) has so far received little scholarly attention, especially in Europe. Consequently, we have very little evidence about how the general public feels about workplace diversity policies – particularly those that are more common or more often discussed in Europe – and about which factors shape public support for these workplace diversity initiatives. Yet, a better understanding of the patterns and antecedents of citizens’ attitudes toward workplace diversity policies is of clear scientific and practical importance. Against this background, this study sheds light on public attitudes toward three different, commonly applied types of workplace diversity policies, and examines which individual-level and – innovatively – national-level conditions shape public support. To do so, we bring together insights from various different and so far largely disconnected strands of research and a range of theoretical perspectives. We use large-scale, representative survey data from two pooled waves of the Eurobarometer, covering 38,009 citizens across 26 European countries. We enrich these data with information on national-level income inequality as well as countries’ labor market and antidiscrimination legislation and policies, obtained from Eurostat and the Migrant Integration Policy Index (MIPEX). Results show significant differences in public support across the three types of policies, with the strongest support for diversity training, followed closely by monitoring recruitment procedures, whereas support for monitoring workforce composition is clearly lower. This is in line with the idea that support tends to be lower for more preferential and prescriptive workplace policies. Furthermore, we find that, in addition to individual-level factors – particularly gender, ethnic minority group membership, personal experiences with discrimination, prejudice, intergroup contact and political orientation – national-level conditions are important antecedents of public support for workplace diversity policies. That is, differences in public attitudes regarding such policies are also shaped by country-level income inequalities, people’s perceptions of how widespread discrimination and unequal opportunities are in society, and national-level laws and policies to fight unequal opportunities.
Longitudinal changes in habitual physical activity in adult people with cystic fibrosis in the presence or absence of treatment with elexacaftor/tezacaftor/ivacaftor
Authors: Gruber Wolfgang , Stehling Florian , Blosch Christopher , Dillenhoefer Stefanie , Olivier Margarete , Brinkmann Folke , Koerner-Rettberg Cordula , Sutharsan Sivagurunathan , Mellies Uwe , Taube Christian , Welsner Matthias
Published: February 2024

Background

Habitual physical activity (PA) and exercise training are accepted as important aspects of care for people with cystic fibrosis (pwCF) to improve health-related measures of physical fitness, which in turn have a positive impact on quality of life and prognosis. In the last decade, effective CFTR modulator therapies have become a promising treatment for pwCF by targeting the underlying cause of CF. This highly effective therapy improves clinical outcomes and quality of life in people with specific CFTR mutations. Little is known about the longitudinal pattern of PA or the impact of the highly effective modulator therapy with Elexacaftor/Tezacaftor/Ivacaftor (ETI) on PA in adult pwCF. This study assessed the course of device-based PA measurement in adult pwCF and evaluated the effects of ETI on habitual physical activity in those who were eligible for ETI.

Methods

Data from adult pwCF (aged ≥18 years) were analysed at baseline and follow-up, using identical assessments at both time points. Outcome parameters were PA in steps/day and the intensity of PA. The group that received ETI was treated for an average of 33 weeks and not for the entire duration of the period. The data were collected between 2021 and 2022, following the removal of absolute pandemic restrictions/lockdowns.

Results

Follow-up duration was 5.6 years in pwCF with ETI (ETI group, n = 21) and 6.5 years in pwCF without ETI (non-ETI group, n = 6). From baseline to follow-up, pwCF treated with ETI had a significant increase in steps/day (+25%, p = 0.019) and a non-significant increase in moderate-to-vigorous intensity time (+5.6%, p = 0.352). Conversely, individuals in the non-ETI group showed a non-significant decrease in both steps/day −3.2%, p = 0.893) and moderate-to-vigorous intensity time (−25%, p = 0.207). The ETI group showed a significant decrease in percent predicted forced expiratory volume in 1 s (ppFEV1) and FEV1 z-score before the start of ETI treatment, both of which improved significantly after therapy initiation. Body weight and body mass index also improved significantly with ETI use.

Conclusions

These data suggest that ETI treatment has a positive effect on habitual physical activity behavior in the adult pwCF studied.
Enhancing algal production strategies: strain selection, AI-informed cultivation, and mutagenesis
Authors: Alzahmi Amnah Salem , Daakour Sarah , Nelson David , Al-Khairy Dina , Twizere Jean-Claude , Salehi-Ashtiani Kourosh
Published: February 2024

Microalgae are emerging as a sustainable source of bioproducts, including food, animal feed, nutraceuticals, and biofuels. This review emphasizes the need to carefully select suitable species and highlights the importance of strain optimization to enhance the feasibility of developing algae as a sustainable resource for food and biomaterial production. It discusses microalgal bioprospecting methods, different types of cultivation systems, microalgal biomass yields, and cultivation using wastewater. The paper highlights advances in artificial intelligence that can optimize algal productivity and overcome the limitations faced in current microalgal industries. Additionally, the potential of UV mutagenesis combined with high-throughput screening is examined as a strategy for generating improved strains without introducing foreign genetic material. The necessity of a multifaceted optimization approach for enhanced productivity is acknowledged. This review provides an overview of recent developments crucial for the commercial success of microalgal production.
Geroscience and pathology: a new frontier in understanding age-related diseases
Authors: Fekete Monika , Major David , Feher Agnes , Fazekas-Pongor Vince , Lehoczki Andrea
Published: February 2024

Geroscience, a burgeoning discipline at the intersection of aging and disease, aims to unravel the intricate relationship between the aging process and pathogenesis of age-related diseases. This paper explores the pivotal role played by geroscience in reshaping our understanding of pathology, with a particular focus on age-related diseases. These diseases, spanning cardiovascular and cerebrovascular disorders, malignancies, and neurodegenerative conditions, significantly contribute to the morbidity and mortality of older individuals. We delve into the fundamental cellular and molecular mechanisms underpinning aging, including mitochondrial dysfunction and cellular senescence, and elucidate their profound implications for the pathogenesis of various age-related diseases. Emphasis is placed on the importance of assessing key biomarkers of aging and biological age within the realm of pathology. We also scrutinize the interplay between cellular senescence and cancer biology as a central area of focus, underscoring its paramount significance in contemporary pathological research. Moreover, we shed light on the integration of anti-aging interventions that target fundamental aging processes, such as senolytics, mitochondria-targeted treatments, and interventions that influence epigenetic regulation within the domain of pathology research. In conclusion, the integration of geroscience concepts into pathological research heralds a transformative paradigm shift in our understanding of disease pathogenesis and promises breakthroughs in disease prevention and treatment.
Rola systemu organów Rady Regencyjnej Królestwa (Państwa) Polskiego w odbudowie państwowości polskiej (wybrane problemy)
Authors: Winnicki Zdzisław
Published: February 2024

The paper refers to selected key political issues related to the construction of central state bodies of the reborn Polish statehood in the years 1917–1918. This construction (organization) was started by activist circles in the proclaimed (reactivated) Kingdom of Poland on November 5, 1916, by emperors of Germany and Austria-Hungary. The emancipatory bodies formed at the time – the Provisional Council of States followed by the Regency Council of the Kingdom of Poland – made efforts to organize the state structures of the reborn statehood, which de facto worked for the future “United Polish State” and as such were taken over and fully used by the sovereign Polish authorities after November 11–14, 1918.
Maize Crop Detection through Geo-Object-Oriented Analysis Using Orbital Multi-Sensors on the Google Earth Engine Platform
Authors: Maciel Junior Ismael Cavalcante , Dallacort Rivanildo , Boechat Cácio Luiz , Teodoro Paulo Eduardo , Teodoro Larissa Pereira Ribeiro , Rossi Fernando Saragosa , Oliveira-Júnior José Francisco de , Della-Silva João Lucas , Baio Fabio Henrique Rojo , Lima Mendelson , Silva Junior Carlos Antonio da
Published: February 2024

Mato Grosso state is the biggest maize producer in Brazil, with the predominance of cultivation concentrated in the second harvest. Due to the need to obtain more accurate and efficient data, agricultural intelligence is adapting and embracing new technologies such as the use of satellites for remote sensing and geographic information systems. In this respect, this study aimed to map the second harvest maize cultivation areas at Canarana-MT in the crop year 2019/2020 by using geographic object-based image analysis (GEOBIA) with different spatial, spectral, and temporal resolutions. MSI/Sentinel-2, OLI/Landsat-8, MODIS-Terra and MODIS-Aqua, and PlanetScope imagery were used in this assessment. The maize crops mapping was based on cartographic basis from IBGE (Brazilian Institute of Geography and Statistics) and the Google Earth Engine (GEE), and the following steps of image filtering (gray-level co-occurrence matrix—GLCM), vegetation indices calculation, segmentation by simple non-iterative clustering (SNIC), principal component (PC) analysis, and classification by random forest (RF) algorithm, followed finally by confusion matrix analysis, kappa, overall accuracy (OA), and validation statistics. From these methods, satisfactory results were found; with OA from 86.41% to 88.65% and kappa from 81.26% and 84.61% among the imagery systems considered, the GEOBIA technique combined with the SNIC and GLCM spectral and texture feature discriminations and the RF classifier presented a mapping of the corn crop of the study area that demonstrates an improved and aided the performance of automated multispectral image classification processes.
Effect of a Lactobacilli-Based Direct-Fed Microbial Product on Gut Microbiota and Gastrointestinal Morphological Changes
Authors: Alawneh John I. , Ramay Hena , Olchowy Timothy , Allavena Rachel , Soust Martin , Jassim Rafat Al
Published: February 2024

The calf’s gastrointestinal tract (GIT) microbiome undergoes rapid shifts during early post-natal life, which can directly affect calf performance. The objectives of this study were to characterise and compare differences in the establishment and succession of GIT microbiota, GIT morphological changes, and the growth of dairy calves from birth until weaned. Forty-four newborn Holstein-Friesian calves were randomly selected and assigned to Treatment (TRT) and Control (CON) groups. The TRT group calves received a once-daily dose of a direct-fed microbial (DFM) liquid product containing Lacticaseibacillus paracasei, Lentilactobacillus buchneri, and Lacticaseibacillus casei, all formerly known as Lactobacillus. Fresh faecal samples were manually taken from the rectum of all calves, and gross necropsy was performed on the forestomachs and gastrointestinal tracts. Bacterial DNA was extracted from frozen faecal samples for 16S rRNA gene amplicon sequencing. Calves in the TRT group had greater live weights (p = 0.02) at weaning compared with calves in the CON group (mean = 69.18 kg, SD = 13.37 kg). The average daily live weight gain (ADG) and total feed intake were similar between the two groups. Calves in the TRT group had greater duodenum, abomasum, and reticulum weights (p = 0.05). Rumen and intestinal development (p < 0.05) and faecal microbial diversity (p < 0.05) were more pronounced in the TRT group. The relative abundances of eight genera differed (p < 0.001) between the groups. Supplementing calves with the LAB-based DFM increased live weight at weaning and had a more pronounced effect on the development of rumen and the gastrointestinal tract and on microbiota diversity and evenness. Future work is needed to better understand the potential association of LAB-DFM products on gut mucosa-associated microbiota.
Animal Welfare Assessment and Meat Quality through Assessment of Stress Biomarkers in Fattening Pigs with and without Visible Damage during Slaughter
Authors: Ogawa Natália Nami , Silva Giovanna Lima , Barbon Ana Paula Ayub da Costa , Flaiban Karina Keller Marques da Costa , Silva Caio Abercio da , Rocha Luiene Moura , Bridi Ana Maria
Published: February 2024

The study aimed to investigate the physiological and meat quality differences between Non-Ambulatory, Non-Injured (NANI), and without apparent abnormalities (non-NANI) pigs in a commercial slaughterhouse setting, focusing on the impact of stress and health conditions on the overall well-being and meat quality of the animals. A total of 241 surgically castrated crossbred male pigs from Southern Brazil were analyzed, with 131 non-NANI pigs and 110 NANI pigs. Infrared orbital temperature, rectal temperature, hematological parameters, and meat quality measurements were collected. Statistical analysis included ANOVA tests and principal component analysis (PCA). NANI pigs exhibited significantly higher infrared orbital temperatures and rectal temperature (p < 0.01). Hematological analysis revealed higher levels of hemoglobin, hematocrit, and red blood cells in NANI pigs (p < 0.05). White blood cell count and lactate dehydrogenase were significantly elevated in NANI pigs (p < 0.01), indicating potential infections or inflammatory responses. Meat quality parameters showed that NANI pigs had lower pH values, higher luminosity, and increased drip loss (p < 0.01), reflecting poorer water retention and potential muscle glycogen depletion. The study highlights the physiological and meat quality differences between NANI and non-NANI pigs, emphasizing the impact of stress, health conditions, and handling procedures on the animals. Blood biomarkers proved valuable in assessing physiological stress, immune response, and potential health issues in pigs, correlating with meat quality abnormalities. Utilizing these biomarkers as predictive tools can enhance animal welfare practices and contribute to improving meat quality in the swine industry.
A Study on the Formation Reactions and Conversion Mechanisms of HONO and HNO3 in the Atmosphere of Daejeon, Korea
Authors: Kim Kyoungchan , Lee Chunsang , Choi Dayeong , Han Sangwoo , Eom Jiwon , Han Jinseok
Published: February 2024

Nitrogen oxides (NOX) in the atmosphere cause oxidation reactions with photochemical radicals and volatile organic compounds, leading to the accumulation of ozone (O3). NOX constitutes a significant portion of the NOy composition, with nitrous acid (HONO) and nitric acid (HNO3) following. HONO plays a crucial role in the reaction cycle of NOX and hydrogen oxides. The majority of HNO3 reduction mechanisms result from aerosolization through heterogeneous reactions, having adverse effects on humans and plants by increasing secondary aerosol concentrations in the atmosphere. The investigation of the formation and conversion mechanisms of HONO and HNO3 is important; however, research in this area is currently lacking. In this study, we observed HONO, HNO3, and their precursor gases were observed in the atmosphere using parallel-plate diffusion scrubber-ion chromatography. A 0-D box model simulated the compositional distribution of NOy in the atmosphere. The formation reactions and conversion mechanisms of HONO and HNO3 were quantified using reaction equations and reaction coefficients. Among the various mechanisms, dominant mechanisms were identified, suggesting their importance. According to the calculation results, the produce of HONO was predominantly attributed to heterogeneous reactions, excluding an unknown source. The sink processes were mainly governed by photolysis during daytime and reactions with OH radicals during nighttime. HNO3 showed dominance in its production from N2O5, and in its conversion mechanisms primarily involving aerosolization and deposition.