bluuebunny/crossref_metadata_2025 huggingface dataset:
Treatment of COVID-19-associated ARDS with mesenchymal stromal cells: a multicenter randomized double-blind trial
Authors: Monsel Antoine , Hauw-Berlemont Caroline , Mebarki Miryam , Heming Nicholas , Mayaux Julien , Nguekap Tchoumba Otriv , Diehl Jean-Luc , Demoule Alexandre , Annane Djillali , Marois Clémence , Demeret Sophie , Weiss Emmanuel , Voiriot Guillaume , Fartoukh Muriel , Constantin Jean-Michel , Mégarbane Bruno , Plantefève Gaëtan , Malard-Castagnet Stéphanie , Burrel Sonia , Rosenzwajg Michelle , Tchitchek Nicolas , Boucher-Pillet Hélène , Churlaud Guillaume , Cras Audrey , Maheux Camille , Pezzana Chloé , Diallo Mamadou Hassimiou , Ropers Jacques , Menasché Philippe , Larghero Jérôme ,   APHP STROMA–CoV-2 Collaborative Research Group, Benchetrit Déborah , Bonvallot Harold , Charbonnier-Beaupel Fanny , Dhib-Charfi Meriem , Delmotte Pierre Romain , Kone Assitan , Le Corre Marine , Marcelin Anne-Geneviève , Metz Carole , Puybasset Louis , Salem Joe-Elie , Vezinet Corinne
Published: February 2022

Background

Severe acute respiratory syndrome coronavirus-2 (SARS–CoV-2)-induced acute respiratory distress syndrome (ARDS) causes high mortality. Umbilical cord-derived mesenchymal stromal cells (UC-MSCs) have potentially relevant immune-modulatory properties, whose place in ARDS treatment is not established. This phase 2b trial was undertaken to assess the efficacy of UC-MSCs in patients with SARS–CoV-2-induced ARDS.

Methods

This multicentre, double-blind, randomized, placebo-controlled trial (STROMA–CoV-2) recruited adults (≥ 18 years) with SARS–CoV-2-induced early (< 96 h) mild-to-severe ARDS in 10 French centres. Patients were randomly assigned to receive three intravenous infusions of 106 UC-MSCs/kg or placebo (0.9% NaCl) over 5 days after recruitment. For the modified intention-to-treat population, the primary endpoint was the partial pressure of oxygen to fractional inspired oxygen (PaO2/FiO2)-ratio change between baseline (day (D) 0) and D7.

Results

Among the 107 patients screened for eligibility from April 6, 2020, to October 29, 2020, 45 were enrolled, randomized and analyzed. PaO2/FiO2 changes between D0 and D7 did not differ significantly between the UC-MSCs and placebo groups (medians [IQR] 54.3 [− 15.5 to 93.3] vs 25.3 [− 33.3 to 104.6], respectively; ANCOVA estimated treatment effect 7.4, 95% CI − 44.7 to 59.7; P = 0.77). Six (28.6%) of the 21 UC-MSCs recipients and six of 24 (25%) placebo-group patients experienced serious adverse events, none of which were related to UC-MSCs treatment.

Conclusions

D0-to-D7 PaO2/FiO2 changes for intravenous UC-MSCs-versus placebo-treated adults with SARS–CoV-2-induced ARDS did not differ significantly. Repeated UC-MSCs infusions were not associated with any serious adverse events during treatment or thereafter (until D28). Larger trials enrolling patients earlier during the course of their ARDS are needed to further assess UC-MSCs efficacy in this context.

Trial registration: NCT04333368. Registered 01 April 2020, https://clinicaltrials.gov/ct2/history/NCT04333368.
A core of differentially methylated CpG loci in gMDSCs isolated from neonatal and adult sources
Authors: Berglund-Brown Isabella , Nissen Emily , Koestler Devin C. , Butler Rondi A. , Eliot Melissa N. , Padbury James F. , Salas Lucas A. , Molinaro Annette M. , Christensen Brock C. , Wiencke John K. , Kelsey Karl T.
Published: February 2022

Background

Myeloid-derived suppressor cells (MDSCs), which include monocytic (mMDSCs) and granulocytic (gMDSCs) cells, are an immunosuppressive, heterogeneous population of cells upregulated in cancer and other pathologic conditions, in addition to normal conditions of stress. The origin of MDSCs is debated, and the regulatory pattern responsible for gMDSC differentiation remains unknown. Since DNA methylation (DNAm) contributes to lineage differentiation, we have investigated whether it contributes to the acquisition of the gMDSC phenotype.

Results

Using the Illumina EPIC array to measure DNAm of gMDSCs and neutrophils from diverse neonatal and adult blood sources, we found 189 differentially methylated CpGs between gMDSCs and neutrophils with a core of ten differentially methylated CpGs that were consistent across both sources of cells. Genes associated with these loci that are involved in immune responses includeVCL, FATS, YAP1, KREMEN2, UBTF,MCC-1, andEFCC1. In two cancer patient groups that reflected those used to develop the methylation markers (head and neck squamous cell carcinoma (HNSCC) and glioma), all of the CpG loci were differentially methylated, reaching statistical significance in glioma cases and controls, while one was significantly different in the smaller HNSCC group.

Conclusions

Our findings indicate that gMDSCs have a core of distinct DNAm alterations, informing future research on gMDSC differentiation and function.
Activated phosphoinositide 3-kinase δ syndrome associated with nephromegaly, growth hormone deficiency, bronchiectasis: a case report
Authors: Craig Megan , Geng Bob , Wigby Kristen , Phillips Susan A. , Bakhoum Christine , Naheedy John , Cernelc-Kohan Mateja
Published: February 2022

Background

Activated phosphoinositide 3-kinase (PI3K) δ syndrome (APDS) is a rare form of primary immunodeficiency with 243 known cases reported in the literature. Known findings associated with the condition include recurrent sinusitis and bronchitis, bronchiectasis, immune cytopenias, mild developmental delay, splenomegaly, and lymphadenopathy. We report the case of a child with APDS accompanied by unique clinical features: nephromegaly and growth hormone deficiency with associated pituitary anatomic abnormality.

Case presentation

The patient is a nine-year-old boy with a heterozygous de novo variant in phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit δ (p.E1021K), previously reported in association with APDS. Our patient, who had no family history of immunodeficiency, exhibits classic findings of this syndrome but also has unique features that extend the phenotypic spectrum of this disorder. At 5 years of age, the patient showed marked growth deceleration and was demonstrated to have growth hormone (GH) deficiency with associated pituitary anatomic abnormality. He started GH therapy with an excellent response. He additionally has bilateral nephromegaly of unclear etiology, microscopic hematuria and proteinuria, asthma, and has developed left hip pain with arthrocentesis consistent with oligoarticular juvenile idiopathic arthritis. At age nine, the patient was referred to genetics and whole exome sequencing revealed APDS. Though there was initial concern that GH may increase risk for malignancy as GH signals through the PI3K pathway, he was allowed to continue treatment as the PI3K pathway was considered constitutively active at baseline.

Conclusions

Our patient’s unique presentation adds to the clinical information regarding APDS, demonstrates the utility of genetic testing and illustrates the importance of a multidisciplinary collaborative approach in managing this complex syndrome.
Telemedicine in allergy/immunology in the era of COVID-19: a Canadian perspective
Authors: Edgerley Sarah , Zhu Rongbo , Quidwai Ariba , Kim Harold , Jeimy Samira
Published: February 2022

Background

In the era of COVID-19, utilization of telemedicine has dramatically increased. In addition to reduced travel times, patient expenses, and work or school days missed, telemedicine allows clinicians to provide continued care while minimizing face-to-face interactions, maintaining social distancing, and limiting potential COVID-19 exposures. Clinical Immunology and Allergy (CIA), like many specialties, has adapted to incorporate telemedicine into practice. Previous studies have demonstrated similar patient satisfaction between virtual and in-person visits. However, evidence from fully publicly funded health care systems such as Canada has been limited.

Methods

We performed a quality improvement (QI) initiative to assess the feasibility of telemedicine. Between 1 March and 30 September 2020, patient encounters of two academic allergists at a single institution in London, Ontario, Canada were analyzed. Assessments were categorized into in-person or telemedicine appointments. A random sample of patients assessed virtually completed a voluntary patient satisfaction survey. Qualitative analysis was performed on survey comments.

Results

In total 3342 patients were seen. The majority were adults (n = 2162, or 64.7%) and female (n = 1872, or 56%). 1543 (46.2%) assessments were virtual and 1799 (53.8%) assessments were in-person. 67 of 100 random patient surveys sent to those in the virtual assessment group were completed. 89.6% (n = 60) agreed or strongly agreed when asked if they were satisfied with their telemedicine visit. 64.2% (n = 43) felt they received the same level of care compared to in-person assessments and 91% (n = 61) stated they would attend another virtual appointment. 95.4% (n = 62) of patients reported saving time with virtual assessment, the majority (n = 42, 62.7%) estimating between 1–4 h saved. Reported shortcomings included technical difficulties, “feeling rushed”, and missing in-person interactions.

Conclusions

Our quality improvement initiative demonstrated high patient satisfaction and time savings with virtual assessment in a publicly funded health care system. Studies suggest that CIA may be uniquely situated to benefit from permanent integration of virtual care into regular practice for both new and follow-up appointments. We anticipate continued increased utilization of telemedicine, signifying a lasting beneficial change in the delivery of healthcare.
A refractory tenosynovitis of the wrist: a case report
Authors: Boudon Alex , Opota Onya , Dan Diana
Published: February 2022

Background

Mycobacterium malmoense is a species of slow-growing nontuberculous mycobacteria. It causes mostly pulmonary infections or lymphadenitis in children, but is increasingly encountered in isolated tenosynovitis in adults. Diagnosis is often delayed because of the rarity of the condition and the difficulty of culturing the bacteria.

Case presentation

We report on a rare association of seronegative polyarthritis with infectious nontuberculous mycobacteria tenosynovitis. A 65-year-old Caucasian female was referred to our clinic because of persisting tenosynovitis of the finger flexor tendons of her right hand, despite two previous synovectomies. She also reported bilateral shoulder and left wrist pain. Paraclinical investigations showed slightly elevated inflammatory parameters. Ultrasound showed synovitis of metacarpophalangeal joints of the right hand and right knee, and a bilateral subacromial bursitis. Hand magnetic resonance imaging also revealed an erosive carpal synovitis. Bacteriological analysis of the second tenosynovectomy specimen showed no growths in aerobic and anaerobic cultures. An additional synovial fluid analysis of the wrist joint was negative for mycobacteria and crystals. Seronegative polyarthritis was suspected, but the initiated immunosuppressive treatment with prednisolone and methotrexate resulted in no clinical improvement of the tenosynovitis. Yet the other joints responded well, and the inflammatory parameters normalized. The immunosuppression was later stopped because of side effects. Due to massive worsening of the tenosynovitis, a third synovectomy was performed. Mycobacterium malmoense was identified on biopsy, leading to the diagnosis of infectious tenosynovitis. At this point, we started an antituberculous therapy, with incomplete response. A combination of antimicrobial and immunosuppressive treatment finally led to the desired clinical improvement.

Conclusion

The treatment of nontuberculous mycobacteria tenosynovitis is not well established, but combining antibiotics with surgical debridement is probably the most adequate approach. Our case highlights the importance of having a high clinical suspicion of an atypical infection in patients with inflammatory tenosynovitis not responding to usual care.
Enhancement strategies for mesenchymal stem cells and related therapies
Authors: Alagesan Senthilkumar , Brady Jack , Byrnes Declan , Fandiño Juan , Masterson Claire , McCarthy Sean , Laffey John , O’Toole Daniel
Published: February 2022

Cell therapy, particularly mesenchymal stem/stromal (MSC) therapy, has been investigated for a wide variety of disease indications, particularly those with inflammatory pathologies. However, recently it has become evident that the MSC is far from a panacea. In this review we will look at current and future strategies that might overcome limitations in efficacy. Many of these take their inspiration from stem cell niche and the mechanism of MSC action in response to the injury microenvironment, or from previous gene therapy work which can now benefit from the added longevity and targeting ability of a live cell vector. We will also explore the nascent field of extracellular vesicle therapy and how we are already seeing enhancement protocols for this exciting new drug. These enhanced MSCs will lead the way in more difficult to treat diseases and restore potency where donors or manufacturing practicalities lead to diminished MSC effect.
Modeling oxidative injury response in human kidney organoids
Authors: Przepiorski Aneta , Vanichapol Thitinee , Espiritu Eugenel B. , Crunk Amanda E. , Parasky Emily , McDaniels Michael D. , Emlet Dave R. , Salisbury Ryan , Happ Cassandra L. , Vernetti Lawrence A. , MacDonald Matthew L. , Kellum John A. , Kleyman Thomas R. , Baty Catherine J. , Davidson Alan J. , Hukriede Neil A.
Published: February 2022

Background

Hemolysis occurs in many injury settings and can trigger disease processes. In the kidney, extracellular hemoglobin can induce damage via several mechanisms. These include oxidative stress, mitochondrial dysfunction, and inflammation, which promote fibrosis and chronic kidney disease. Understanding the pathophysiology of these injury pathways offers opportunities to develop new therapeutic strategies.

Methods

To model hemolysis-induced kidney injury, human kidney organoids were treated with hemin, an iron-containing porphyrin, that generates reactive oxygen species. In addition, we developed an induced pluripotent stem cell line expressing the biosensor, CytochromeC-GFP (CytoC-GFP), which provides a real-time readout of mitochondrial morphology, health, and early apoptotic events.

Results

We found that hemin-treated kidney organoids show oxidative damage, increased expression of injury markers, impaired functionality of organic anion and cation transport and undergo fibrosis. Injury could be detected in live CytoC-GFP organoids by cytoplasmic localization of fluorescence. Finally, we show that 4-(phenylthio)butanoic acid, an HDAC inhibitor with anti-fibrotic effects in vivo, reduces hemin-induced human kidney organoid fibrosis.

Conclusion

This work establishes a hemin-induced model of kidney organoid injury. This platform provides a new tool to study the injury and repair response pathways in human kidney tissue and will assist in the development of new therapeutics.
Porcine reproductive and respiratory virus 2 infection of the fetus results in multi-organ cell cycle suppression
Authors: Mulligan Margaret K. , Kleiman Jocelyn E. , Caldemeyer Andrew C. , Harding John C. S. , Pasternak J. Alex
Published: February 2022

Porcine reproductive and respiratory syndrome virus (PRRSV) infection during late gestation negatively affects fetal development. The objective of this study was to identify the fetal organs most severely impacted following infection, and evaluate the relationship between this response and fetal phenotypes. RNA was extracted from fetal heart, liver, lung, thymus, kidney, spleen, and loin muscle, collected following late gestation viral challenge of pregnant gilts. Initially, gene expression for three cell cycle promoters (CDK1, CDK2, CDK4) and one inhibitor (CDKN1A) were evaluated in biologically extreme phenotypic subsets including gestational age-matched controls (CON), uninfected (UNIF), high-viral load viable (HV-VIA), and high-viral load meconium-stained (HV-MEC) fetuses. There were no differences between CON and UNIF groups for any gene, indicating no impact of maternal infection alone. Relative to CON, high-viral load (HV-VIA, HV-MEC) fetuses showed significant downregulation of at least one CDK gene in all tissues except liver, while CDKN1A was upregulated in all tissues except muscle, with the heart and kidney most severely impacted. Subsequent evaluation of additional genes known to be upregulated following activation of P53 or TGFb/SMAD signaling cascades indicated neither pathway was responsible for the observed increase in CDKN1A. Finally, analysis of heart and kidney from a larger unselected population of infected fetuses from the same animal study showed that serum thyroxin and viral load were highly correlated with the expression of CDKN1A in both tissues. Collectively these results demonstrate the widespread suppression in cell division across all tissues in PRRSV infected fetuses and indicate a non-canonical regulatory mechanism.
A remark on Secelean–Wardowski’s fixed point theorems
Authors: Saejung Satit , Ardsalee Pinya
Published: February 2022

In this paper we give a simple proof of three fixed point theorems of Secelean and Wardowski by using the fixed point result of Jachymski et al. Our result is established with weaker assumptions than the three theorems. Furthermore, the recent result of Secelean et al. in the setting of a complete metric space can be also deduced by our theorem.
Effect of market-driven strategies on the competitive growth of SMEs in Lesotho
Authors: Amadasun Donald O. E. , Mutezo Ashley. T.
Published: February 2022

This paper established some market-driven strategies that influence the competitive growth of small and medium-sized enterprises in Lesotho. The paper identified the following factors; market orientation, competitive intensity, and technological dynamics as variables that influence SMEs’ competitive growth. The investigation shows that these critical factors of market-driven strategies are significant market facets in the enterprise that could capacitate SME entrepreneurs and managers to attain competitive growth. The results indicated that the factors used to measure market-driven strategies influence SMEs competitive growth. More specifically, the independent variables of market orientation, competitive intensity and technological dynamics are seen as key tenets of market-driven strategies that influence small and medium-sized enterprises’ competitive growth. From the analyses, this paper recommended that market-driven strategic constructs of market orientation, competitive intensity and technological dynamics are inimitable and tangible significant resources if harnessed in the enterprises, could serve as critical operational factors that influence SMEs competitive growth in Lesotho.
Early life inter-kingdom interactions shape the immunological environment of the airways
Authors: Pattaroni Céline , Macowan Matthew , Chatzis Roxanne , Daunt Carmel , Custovic Adnan , Shields Michael D. , Power Ultan F. , Grigg Jonathan , Roberts Graham , Ghazal Peter , Schwarze Jürgen , Gore Mindy , Turner Steve , Bush Andrew , Saglani Sejal , Lloyd Clare M. , Marsland Benjamin J.
Published: February 2022

Background

There is increasing evidence that the airway microbiome plays a key role in the establishment of respiratory health by interacting with the developing immune system early in life. While it has become clear that bacteria are involved in this process, there is a knowledge gap concerning the role of fungi. Moreover, the inter-kingdom interactions that influence immune development remain unknown. In this prospective exploratory human study, we aimed to determine early post-natal microbial and immunological features of the upper airways in 121 healthy newborns.

Results

We found that the oropharynx and nasal cavity represent distinct ecological niches for bacteria and fungi. Breastfeeding correlated with changes in microbiota composition of oropharyngeal samples with the greatest impact upon the relative abundance ofStreptococcusspecies andCandida. Host transcriptome profiling revealed that genes with the highest expression variation were immunological in nature. Multi-omics factor analysis of host and microbial data revealed unique co-variation patterns.

Conclusion

These data provide evidence of a diverse multi-kingdom microbiota linked with local immunological characteristics in the first week of life that could represent distinct trajectories for future respiratory health.

Trial registration

NHS Health Research Authority, IRAS ID 199053. Registered 5 Oct 2016.https://www.hra.nhs.uk/planning-and-improving-research/application-summaries/research-summaries/breathing-together/
From efficacy to effectiveness: child and adolescent eating disorder treatments in the real world (part 1)—treatment course and outcomes
Authors: Simic Mima , Stewart Catherine S. , Konstantellou Anna , Hodsoll John , Eisler Ivan , Baudinet Julian
Published: February 2022

Background

Findings from randomised control trials inform the development of evidence-based eating disorder (ED) practice guidelines internationally. Only recently are data beginning to emerge regarding how these treatments perform outside of research settings. This study aimed to evaluate treatment pathways and outcomes for a specialist child and adolescent ED service across a five-year period.

Methods

All consecutive referrals between August 2009 and January 2014 seen at the Maudsley Centre for Child and Adolescent Eating Disorders in London were included. Data are reported on for all young people who were offered treatment (N = 357).

Results

Most young people referred to the service were diagnosed with anorexia nervosa (AN)/Atypical AN (81%). Treatment for AN/Atypical AN (median 11 months) was predominantly ED focused family therapy (99%). Treatment for bulimia nervosa (BN)/Atypical BN (median seven months) was most commonly a combination of cognitive behavioural therapy and ED focused family therapy (87%). At discharge, 77% of the AN/Atypical AN group had a good or intermediate outcome and 59% of the BN/Atypical BN group reported no or fewer than weekly bulimic episodes. 27% of the AN/Atypical AN group had enhanced treatment with either day- and/or inpatient admissions (AIM group). The %mBMI at 3 months of treatment was strongest predictor of the need for treatment enhancement and more modestly EDE-Q and age at assessment. The AIM group at assessment had significantly lower weight, and higher ED and comorbid symptomatology and went on to have significantly longer treatment (16 vs. 10 months). At discharge, this group had significantly fewer good and more poor outcomes on the Morgan Russell criteria, but similar outcomes regarding ED and comorbid symptoms and quality of life. When analysis was adjusted for %mBMI at assessment, 1 and 3 months of treatment, differences in Morgan Russell outcomes and %mBMI were small and compatible with no difference in outcome by treatment group.

Conclusions

This study shows that outcomes in routine clinical practice in a specialist community-based service compare well to those reported in research trials. The finding from research trials that early weight gain is associated with improved outcomes was also replicated in this study. Enhancing outpatient treatment with day treatment and/or inpatient care is associated with favourable outcome for most of the young people, although a longer duration of treatment is required.
The message of oldhamites from enstatite chondrites
Authors: Hammouda Tahar , Boyet Maud , Frossard Paul , Cartier Camille
Published: February 2022

We have determined rare-earth element (REE) abundances in oldhamites (CaS) from 13 unequilibrated and equilibrated enstatite chondrites (5 EH and 8 EL) and in a few enstatites by in situ, laser ablation ICP-MS. In EH chondrites, oldhamite REE patterns vary from the most primitive petrographic types (EH3) to the most metamorphosed types (EH5). In EH3, CI-normalized REE patterns are convex downward with strong positive Eu and Yb anomalies, whereas EH5 display flat patterns with enrichments reaching about 80 times CI abundances. The positive anomalies of Eu and Yb found in oldhamites of primitive EH chondrites indicate that they represent the condensation of a residual gas fraction, in a manner similar to fine-grained CAIs of carbonaceous chondrites. The early condensate may have been preserved in the matrix of unequilibrated EH. Equilibrated EH oldhamite patterns may result from metamorphic evolution and REE redistribution on the EH parent body. On the contrary, all the oldhamites from EL chondrites (EL3 to EL6) display a single kind of patterns, which is convex upward and is about 100 times enriched relative to CI, with a negative Eu anomaly. In addition, the EL pattern is similar to that of oldhamites from aubrites (enstatite achondrites). The latter observation suggests that oldhamites of all EL metamorphic types (including primitive ones) bear the signature of a magmatic event accompanied by FeS loss as vapor, prior to the assembly of the EL parent body. Given the difficulty of obtaining precise ages on enstatite chondrites, it is not possible to discuss the chronology of the events recorded by the oldhamite REE patterns.
Torrent rainfall-induced large-scale karst limestone slope collapse at Khanh waterfall, Hoa Binh Province, Vietnam
Authors: Do Ngoc Ha , Goto Satoshi , Abe Shinro , Nguyen Kim Thanh , Miyagi Toyohiko , Hayashi Kazunori , Watanabe Osamu
Published: February 2022

In recent years, many landslides have occurred in Vietnam, particularly in the Northern mountainous region during the rainy season from May to October. On the morning of October 12, 2017, the Khanh waterfall landslide in Khanh Village, Hoa Binh Province, Northern Vietnam occurred. The landslide killed eighteen people and destroyed five houses. Topographical and geological surveys were conducted around the area to determine its causes. The rainfall data and flow discharge were also analyzed. The results showed that this collapse was different from some previous ones collapsed due to the erosion at the toe of the slope. Khanh waterfall landslide occurred due to the increasing amount of water in cracks and caves in the limestone layer in the slope. The collapse process was simulated based on Coulomb mixture theory. The numerical simulation results show similarities with the actual collapse process. The results provide indicators for assessing the risk of such limestone waterfall landslides in the future.
My nutrition index: a method for measuring optimal daily nutrient intake
Authors: Busgang Stefanie A. , Malin Ashley J. , Gennings Chris
Published: February 2022

Background

Adequate nutrition is essential for individual and population level health. However, determining adequacy of daily nutrient intake in research studies is often challenging given the unique nutritional needs of individuals. Herein, we examine construct, predictive, criterion, content, and concurrent validity of a dietary analytic tool – My Nutrition Index (MNI) for measuring nutrient intake in relation to personalized daily nutrient intake guidelines. MNI gauges adequacy of an individual’s daily nutrient intake based on his or her unique demographic and lifestyle characteristics. MNI accounts for potential adverse effects of inadequate and excess nutrient consumption.

Methods

MNI, calculated based on 34 nutrients, provides an overall index score ranging from 0 to 100, with higher scores reflecting a more nutritious diet. We calculated MNI scores for 7154 participants ages 18-65 in the National Health and Nutrition Examination Surveys (2007-2014) by using average nutrient intakes from two 24-h dietary recalls. Survey-weighted binary logistic regression models were used to assess associations between MNI scores and obesity, depression, health perceptions, and past or present cardiovascular disease.

Results

Higher MNI scores were associated with lower prevalence of self-reported cardiovascular disease (OR = 0.69, CI: 0.52, 0.92, p = 0.012), depression (OR = 0.76, CI: 0.65, 0.90, p < 0.001), and obesity (OR = 0.92, CI: 0.87, 0.99, p = 0.016), as well as more favorable health perceptions (OR = 1.24, CI: 1.13, 1.37, p < 0.001).

Conclusions

MNI provides an individualized approach for measuring adequacy/sufficiency of daily nutrient intake that can validly be employed to assess relationships between nutrition and health outcomes in research studies.
A preliminary study of the efficacy of the polyherbal preparation Sao Thong Tai for erectile dysfunction among elderly men: a double-blind, randomized controlled trial
Authors: Lin Chakariya , Pattraraachachai Junya , Pawa Kammal K. , Wongyai Surapote
Published: February 2022

Background

The traditional Thai polyherbal formulation “Sao Thong Tai” (STT) contains four medicinal plants, namely Boesenbergia rotunda (L.) Mansf., Sida acuta Burm.f., Dactyloctenium aegyptium (L.) Willd., and Oryza sativa L. and it is considered an aphrodisiac and remedy for the effective treatment of decreased male libido in Thai traditional medicine and it may ameliorate erectile function. This pilot study was carried out to investigate the efficacy and safety of the polyherbal STT for mild to moderate erectile dysfunction in a small group of elderly men in Thailand in a double-blind, randomized controlled trial.

Methods

This research is a preliminary study. Fifty-four elderly males aged 50-69 years who had been diagnosed with mild to moderate ED were randomized into two groups of equal size: an STT group (n=27) and a placebo control group (n=27). The patients received either an 800 mg dose of STT extract or a lactose placebo twice a day for the eight-week treatment period, after which they were followed up for four weeks. Erectile function, sexual activity, self-reported attitudes toward sexual dysfunction, and partner-reported sexual dysfunction were investigated to confirm the efficacy of the polyherbal preparation. The International Index of Erectile Function (IIEF-5) and the Scale for Quality of Sexual Function (QSF) were used for primary outcome assessment.

Results

The IIEF-5 scores of the STT group were significantly increased compared with those of the placebo group (p<0.001). The severity of ED decreased from "mild" or "mild to moderate" to normal in 88.9% of the STT group vs. 0% of the placebo group, and the QSF score increased in the STT group (p<0.001). Sexual activity, self-reported views of sexual dysfunction, and partners’ views of sexual dysfunction showed significant improvement in the STT group, and there was no significant difference in psychosomatic quality of life. No adverse events were observed in either group.

Conclusion

Compared with a placebo, an oral aqueous extract of STT taken daily at a dose of 1,600 mg for eight weeks can effectively treat mild to moderate ED in elderly men. The preparation was also shown to be safe with respect to all parameters assessed. This finding demonstrates that STT can be used for the effective treatment of decreased male libido in Thai traditional medicine and may ameliorate ED.

Trial registration

TCTR/20180126001 033/2560. Registered 16 January 2018, Thai Clinical Trials Registry http://www.thaiclinicaltrials.org/
Household food security during the COVID-19 pandemic in urban and semi-urban areas in Indonesia
Authors: Syafiq Ahmad , Fikawati Sandra , Gemily Syilga Cahya
Published: February 2022

Background

One of the impacts of the COVID-19 pandemic was the weakening of the community's economic condition. The weak economy of the community will have an impact on household food security. This study aims to determine food security in the COVID-19 pandemic situation and the impact of the pandemic on food security in urban and semi-urban areas.

Methods

A cross-sectional study with a total sample of 517 people who live in urban (Jakarta) and semi-urban (Depok) areas. The research data was collected online and purposively through Posyandu cadres who have access to family/community. Food security was measured using HFIAS (Household Food Insecurity Access Scale) method, while impact of COVID-19 pandemic was categorized into two categories: impacted (reduced income and laid off) and less impacted (not reduced income and laid off). Data analysis used the Chi-square test and multiple logistic regression.

Results

There were 65.0% of households with various level of food insecurity during the COVID-19 pandemic. The results of the multivariate analysis showed that family income during the COVID-19 pandemic (AOR = 4.2; CI = 2.7–6.7), the type of impact of the COVID-19 pandemic, i.e., reduced income and stopped working (AOR = 2.6; CI = 1.6–4.1), and the age of the respondent (AOR = 1.7; CI = 1.1–2.5) were significantly related to household food security during the pandemic after being controlled by husband's work status. Households with lower income had 4 times higher risk to experience food insecurity compared to those with higher income. Heavily impacted households (through reduced income and stopped working) had 3 times higher risk to experience food insecurity compared to those who did not. Additionally, we found that households with younger respondent (< 31 years old) had 2 times higher risk to experience food insecurity compared to those older counterparts.

Conclusions

The COVID-19 pandemic impacted household food security in both urban and semi-urban areas through worsening employment status and income condition.
Identification of a novel four-gene diagnostic signature for patients with sepsis by integrating weighted gene co-expression network analysis and support vector machine algorithm
Authors: Li Mingliang , Huang He , Ke Chunlian , Tan Lei , Wu Jiezhong , Xu Shilei , Tu Xusheng
Published: February 2022

Sepsis is a life-threatening condition in which the immune response is directed towards the host tissues, causing organ failure. Since sepsis does not present with specific symptoms, its diagnosis is often delayed. The lack of diagnostic accuracy results in a non-specific diagnosis, and to date, a standard diagnostic test to detect sepsis in patients remains lacking. Therefore, it is vital to identify sepsis-related diagnostic genes. This study aimed to conduct an integrated analysis to assess the immune scores of samples from patients diagnosed with sepsis and normal samples, followed by weighted gene co-expression network analysis (WGCNA) to identify immune infiltration-related genes and potential transcriptome markers in sepsis. Furthermore, gene regulatory networks were established to screen diagnostic markers for sepsis based on the protein-protein interaction networks involving these immune infiltration-related genes. Moreover, we integrated WGCNA with the support vector machine (SVM) algorithm to build a diagnostic model for sepsis. Results showed that the immune score was significantly lower in the samples from patients with sepsis than in normal samples. A total of 328 and 333 genes were positively and negatively correlated with the immune score, respectively. Using the MCODE plugin in Cytoscape, we identified four modules, and through functional annotation, we found that these modules were related to the immune response. Gene Ontology functional enrichment analysis showed that the identified genes were associated with functions such as neutrophil degranulation, neutrophil activation in the immune response, neutrophil activation, and neutrophil-mediated immunity. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed the enrichment of pathways such as primary immunodeficiency, Th1- and Th2-cell differentiation, T-cell receptor signaling pathway, and natural killer cell-mediated cytotoxicity. Finally, we identified a four-gene signature, containing the hub genesLCK,CCL5,ITGAM, andMMP9, and established a model that could be used to diagnose patients with sepsis.
Characteristics of ‘mega’ peer-reviewers
Authors: Rice Danielle B. , Pham Ba’ , Presseau Justin , Tricco Andrea C. , Moher David
Published: February 2022

Background

The demand for peer reviewers is often perceived as disproportionate to the supply and availability of reviewers. Considering characteristics associated with peer review behaviour can allow for the development of solutions to manage the growing demand for peer reviewers. The objective of this research was to compare characteristics among two groups of reviewers registered in Publons.

Methods

A descriptive cross-sectional study design was used to compare characteristics between (1) individuals completing at least 100 peer reviews (‘mega peer reviewers’) from January 2018 to December 2018 as and (2) a control group of peer reviewers completing between 1 and 18 peer reviews over the same time period. Data was provided by Publons, which offers a repository of peer reviewer activities in addition to tracking peer reviewer publications and research metrics. Mann Whitney tests and chi-square tests were conducted comparing characteristics (e.g., number of publications, number of citations, word count of peer review) of mega peer reviewers to the control group of reviewers.

Results

A total of 1596 peer reviewers had data provided by Publons. A total of 396 M peer reviewers and a random sample of 1200 control group reviewers were included. A greater proportion of mega peer reviews were male (74%) as compared to the control reviewers (58% male). Mega peer reviewers demonstrated a significantly greater average number of total publications, citations, receipt of Publons awards, and a higher average h index as compared to the control group of reviewers (all p < .001). We found no statistically significant differences in the number of words between the groups (p > .428).

Conclusions

Mega peer reviewers registered in the Publons database also had a higher number of publications and citations as compared to a control group of reviewers. Additional research that considers motivations associated with peer review behaviour should be conducted to help inform peer reviewing activity.
Coordination of dual setting overcurrent relays in microgrid with optimally determined relay characteristics for dual operating modes
Authors: Tiwari Raghvendra , Singh Ravindra Kumar , Choudhary Niraj Kumar
Published: February 2022

Fault current magnitude in a microgrid depends upon its mode of operation, namely, grid-connected mode or islanded mode. Depending on the type of fault in a given mode, separate protection schemes are generally employed. With the change in microgrid operating mode, the protection scheme needs to be modified which is uneconomical and time inefficient. In this paper, a novel optimal protection coordination scheme is proposed, one which enables a common optimal relay setting which is valid in both operating modes of the microgrid. In this context, a common optimal protection scheme is introduced for dual setting directional overcurrent relays (DOCRs) using a combination of various standard relay characteristics. Along with the two variables, i.e., time multiplier setting (TMS) and plug setting (PS) for conventional directional overcurrent relay, dual setting DOCRs are augmented with a third variable of relay characteristics identifier (RCI), which is responsible for selecting optimal relay characteristics from the standard relay characteristics according to the IEC-60255 standard. The relay coordination problem is formulated as a mixed-integer nonlinear programming (MINLP) problem, and the settings of relays are optimally determined using the genetic algorithm (GA) and the grey wolf optimization (GWO) algorithm. To validate the superiority of the proposed protection scheme, the distribution parts of the IEEE-14 and IEEE-30 bus benchmark systems are considered.
Cancer treatment regimens and their impact on the patient-reported outcome measures health-related quality of life and perceived cognitive function
Authors: Grusdat Niklas Paul , Stäuber Alexander , Tolkmitt Marion , Schnabel Jens , Schubotz Birgit , Wright Peter Richard , Heydenreich Marc , Zermann Dirk-Henrik , Schulz Henry
Published: February 2022

Background and purpose

Breast cancer can be a significant challenge for those affected. Knowledge of physical function, social-emotional challenges, and perceived cognitive function based on the cancer treatment regimens may help to inform adequate support.

Methods

For this prospective observational pilot study, we collected data of seventy-nine women (mean age 54.6 ± 9.5 years) before (T0) and after (T1) initial breast cancer treatment. Functional Assessment of Cancer Therapy-Breast (FACT-B) and Functional Assessment of Cancer Therapy–Cognitive-Function (FACT-Cog) were used to collect data of four treatment subgroups: SCR = Surgery + Chemotherapy + Radiation Therapy; SC = Surgery + Chemotherapy; SR = Surgery + Radiation Therapy; S = Surgery. A mixed ANOVA and posthoc analysis (Tukey, Games-Howell) were used to detect interactions (group by time) and the main effect. A repeated-measures ANOVA displayed individual group differences (time).

Results

Significant interaction showed more deterioration was experienced with SC and SCR than SR and S for FACT-B (p < 0.01) and FACT-Cog (p < 0.001). The longitudinal comparison between T0 and T1 indicated a significant group main effect on all subscales (p < 0.001) except for Emotional Well-Being. Significant reductions (p < 0.05) in FACT-B, (− 19%); FACT-Cog, (− 21%) with most pronounced effect in Physical Well-Being (− 30%), Functional Well-Being (− 20%), Breast Cancer Subscale (− 20%), Perceived Cognitive Impairments (− 18%) and Impact of Cognitive Impairments on Quality of Life (− 39%) were detected for SCR.

Conclusion

Our study showed that the extent of change in health-related quality of life (HRQoL) and perceived cognitive function (PCF) depends on the treatment regimen. Multidisciplinary support initiated early in breast cancer therapy is needed, especially for women undergoing combined cancer treatment. Routine assessment of patient-reported outcomes (PROs) in oncology practice may increase the transparency of patients’ perceived circumstances, leading to personalized and optimized acute and survivorship care.
Effect of waist circumference and body mass index on the level of spinal anesthesia
Authors: Günkaya Mustafa , Arar Cavidan , Mordeniz Cengiz , Baran Onur , Topçu Birol
Published: February 2022

Background

We aimed to determine the effects of waist circumference and body mass index on spinal anesthesia levels. In total, 120 surgical patients who were between 18 and 65 years old and in the American Society of Anesthesiologists’ (ASA) I–III risk groups enrolled in this study. Patients were classified into three groups, depending on their weight. After a spinal block, we noted the time needed for the sensory block to reach the T10 level, the maximum sensory block level, the time needed for the sensory block to reach the maximum sensory block level, the time needed before the start of the motor block, and the Bromage scale for each patient.

Results

We observed no significant demographic differences in age, gender, or ASA risk class between the groups; however, we found a statistically significant difference between the groups’ BMIs and waist circumferences. For the time needed for the spinal block to reach the T10 level, we observed a statistically significant difference between groups I, II, and III, and we also found a statistically significant difference between the groups’ comparing Bromage scales. Moreover, we found a statistically significant difference between the groups’ time needed (in minutes) for the block to reach the maximal upper dermatomal block level and, as BMIs and waist circumferences increased, the time needed to reach the maximal upper dermatomal block level. We also noted a statistically significant difference in waist circumference variability.

Conclusions

This study shows that body mass index and waist circumference can be used and interpreted as independent parameters reflecting the increasing incidence of obesity.
Numerical simulation of a complex moving rigid body under the impingement of a shock wave in 3D
Authors: Liu Shihao , Cheng Ziqiang , Jiang Yan , Lu Jianfang , Zhang Mengping , Zhang Shuhai
Published: February 2022

In this paper, we take a numerical simulation of a complex moving rigid body under the impingement of a shock wave in three-dimensional space. Both compressible inviscid fluid and viscous fluid are considered with suitable boundary conditions. We develop a high order numerical boundary treatment for the complex moving geometries based on finite difference methods on fixed Cartesian meshes. The method is an extension of the inverse Lax-Wendroff (ILW) procedure in our works (Cheng et al., Appl Math Mech (Engl Ed) 42: 841–854, 2021; Liu et al.) for 2D problems. Different from the 2D case, the local coordinate rotation in 3D required in the ILW procedure is not unique. We give a theoretical analysis to show that the boundary treatment is independent of the choice of the rotation, ensuring the method is feasible and valid. Both translation and rotation of the body are taken into account in this paper. In particular, we reformulate the material derivative for inviscid fluid on the moving boundary with no-penetration condition, which plays a key role in the proposed algorithm. Numerical simulations on the cylinder and sphere are given, demonstrating the good performance of our numerical boundary treatments.
Kinetic modelling of in situ treatment of petroleum hydrocarbon contaminated soil using bone char and NPK fertilizers
Authors: Okwonna Obumneme O. , Otaraku Ipeghan J.
Published: February 2022

This study investigates the effectiveness of bone char (organic) and inorganic nitrogen, phosphorous, and potassium (NPK) fertilizers as stimulants in the degradability of petroleum hydrocarbon contaminants on soil. The physicochemical properties of the hydrocarbon sludge were used to assess the effectiveness of this process over an 8-week period using 0.5–3.5 kg mass of each fertilizer at different experiments. A first order kinetic model was used to estimate the rate of degradation of the total hydrocarbon content (THC) and total organic carbon (TOC) contaminants and the half-life of the remediation process. The microbial population within the period was also determined. The p-value (p < 0.05) indicated that these fertilizers were effective in degrading these contaminants on the soil, because of the significant difference between the treated and the control soil samples. A direct relationship was observed between with the mass and performance of the fertilizers. With 3.5 kg mass of the fertilizers, rate constants of 0.018 and 0.019 d− 1 were obtained for the removal of the THC and TOC contaminants, respectively, using the bone char fertilizer, whereas NPK fertilizer gave rates of 0.03 and 0.023 d− 1 respectively. The performance of the NPK fertilizer is attributable to its nitrogen and phosphorous content. The model adequately described the process and showed the effectiveness of both fertilizers in the remediation process.