bluuebunny/crossref_metadata_2025 huggingface dataset:
Utilization of Electronic Health Record Events to Conduct a Tuberculosis Contact Investigation in a High-Risk Oncology Unit
Authors: Usiak Shauna C. , Romero Fabian A. , Schwegman Patrice , Fitzpatrick Violet , Connor MaryAnn , Eagan Janet , Brown Arthur E. , Kamboj Mini
Published: August 2017

OBJECTIVE

To describe the utilization of electronic medical data resources, including health records and nursing scheduling resources, to conduct a tuberculosis (TB) exposure investigation in a high-risk oncology unit.

SETTING

A 42-bed inpatient unit with a mix of surgical and medical patients at a large tertiary-care cancer center in New York City.

PARTICIPANTS

High-risk subjects and coworkers exposed to a healthcare worker (HCW) with cavitary smear positive lung TB.

RESULTS

During the 3-month exposure period, 270 patients were admitted to the unit; 137 of these (50.7%) received direct care from the index case HCW. Host immune status and intensity of exposure were used to establish criteria for postexposure testing, and 63 patients (45%) met these criteria for first-tier postexposure testing. No cases of active TB occurred. Among coworkers, 146 had significant exposure (ie, >8 hours cumulative). In the 22-month follow-up period after the exposure, no purified protein derivative or interferon gamma release assay conversions or active cases of TB occurred among exposed HCWs or patients.

CONCLUSIONS

Electronic medical records and employee scheduling systems are useful resources to conduct otherwise labor-intensive contact investigations. Despite the high-risk features of our index case, a highly vulnerable immunocompromised patient population, and extended proximity to coworkers, we did not find any evidence of transmission of active or latent tuberculosis infection among exposed individuals.

Infect Control Hosp Epidemiol2017;38:1235–1239
Decontamination of Hospital Surfaces With Multijet Cold Plasma: A Method to Enhance Infection Prevention and Control?
Authors: Cahill Orla J. , Claro Tânia , Cafolla Attilio A. , Stevens Niall T. , Daniels Stephen , Humphreys Hilary
Published: August 2017

OBJECTIVE

To evaluate the efficacy of a multijet cold-plasma system and its efficacy in decontaminating 2 surfaces commonly found in hospitals

DESIGN

An in vitro study of common causes of healthcare-acquired infection

METHODS

Log10 9 cultures of methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, extended spectrum β-lactamase–producing Escherichia coli, and Acinetobacter baumannii were applied to 5-cm2 sections of stainless steel and mattress. Human serum albumin (HSA) was used as a proxy marker for organic material, and atomic force microscopy (AFM) was used to study the impact on bacterial cell structure. The inoculated surfaces were exposed to a cold-air-plasma–generating multijet prototype for 15, 20, 30, and 45 seconds.

RESULTS

After 45 seconds, at least 3 to 4 log reductions were achieved for all bacteria on the mattress, while 3 to 6 log reductions were observed on stainless steel. The presence of HSA had no appreciable effect on bacterial eradication. The surfaces with bacteria exposed to AFM showed significant morphological changes indicative of “etching” due to the action of highly charged ions produced by the plasma.

CONCLUSION

This multijet cold-plasma prototype has the potential to augment current environmental decontamination approaches but needs further evaluation in a clinical setting to confirm its effectiveness.

Infect Control Hosp Epidemiol 2017;38:1182–1187
Investigating geospatial models of the diffusion of morphosyntactic innovations: The Welsh strong second-person singular pronounchdi
Authors: Willis David
Published: August 2017

Morphosyntactic dialect variation, once a neglected area of dialect research, has recently witnessed a large growth in interest. Various methods from geospatial data analysis have been applied to morphosyntactic data. To date, the focus has largely been on analyzing the distribution of stable patterns of variation. This article extends this work to examine patterns of ongoing change. It uses a body of data from the Syntactic Atlas of Welsh Dialects and the Siarad Corpus of spoken Welsh to examine the innovation and diffusion of a new second-person singular pronoun,chdi, testing the usefulness of Geographically Weighted Regression (GWR) as a method for identifying and modeling patterns of ongoing syntactic change. It is shown that GWR provides plausible models of the diachronic development of changes that are still in progress. Furthermore, it allows us to test whether rates of change are constant across geographical space, allowing us to test whether the Constant Rate Hypothesis (that diffusion of change proceeds at the same rate in different environments) holds between dialects.
Infidel Deathbeds: Irreligious Dying and Sincere Disbelief in Nineteenth-Century America
Authors: Kime Bradley
Published: August 2017

This article inquires into the piecemeal, provisional de-marginalization of American irreligion and analyzes the social stakes and strategies of dis/belief's invocation during the long nineteenth century. It does so by considering the era's corpus of American deathbed narratives. It argues that late-century irreligionists mimed and subverted the deathbed strategies of their Christian detractors to convince a skeptical American audience to concede the contested sincerity of their disbelief. For much of the nineteenth century, Christian-produced infidel deathbed narratives mapped the mixture and multiplicity of inner irreligion and interrogated the sincerity of disbelief. In response, irreligionists—initially ambivalent about the interpretability of the deathbed—eventually came to invest it with as much power to prove sincerity as had American Christians. In the last quarter of the nineteenth century, irreligionists developed a nationwide network of irreligious dying and selectively, strategically deployed the deathbed's accrued power to prove the uniform sincerity of their disbelief. By the turn of the century, they had largely neutralized the derisive force of the infidel deathbed genre, leaving disbelief a partially, provisionally less marginal and less multiplex marker in American society, and re-tethering themselves to their Christian detractors in the process.
A new species ofSpiogalea(Polychaeta: Spionidae) from Brazil, with an amended diagnosis of the genus
Authors: Peixoto Antonio J.M. , Paiva Paulo C.
Published: August 2017

A newSpiogaleaspecies is described from South-east Brazil. The specimens were collected in muddy bottoms during a large survey (15–3000 m) in southern Brazil. This genus comprised, up to now, only one species described from Capbreton Canyon (Bay of Biscay). The new species differs from the type-species, mostly in the morphology of the prostomial chitinous plate, a character restricted to the genusSpiogalea. With this new species, the generic diagnosis of the genus was amended.
Revisited Syllidae of the English Channel coarse sediment communities
Authors: Pezy Jean-Philippe , Baffreau Alexandrine , Dauvin Jean-Claude
Published: August 2017

Among the polychaetes, the Syllidae comprise numerous species whose study over many years has benefitted from valuable revisions and descriptions of new species in Europe. This abundant literature proves very useful for revisiting the taxonomy and distribution of the Syllidae in the English Channel (EC), mainly as regards existing studies on coarse sediment communities in the eastern part of the Channel. This habitat is one of the most widespread in the EC and is known as favourable for the small polychaete fauna including Syllidae. A 2-year survey (winter and summer sampling periods) covering 19 stations, associated with theBranchiostoma lanceolatumcoarse sand community offshore Dieppe-Le Tréport, led to the identification of 6537 individuals from 29 taxa including 27 species. Six fine sand stations were also sampled in which only 12 individuals were collected. Among these species, seven are new for the EC polychaete fauna and six others are observed for the first time in the eastern part of the EC. All the new species for the EC are warm temperate species previously only known south of the Bay of Biscay. The Syllidae list given in Dauvinet al. (2003) has been re-analysed and amended with our list and that of the Chausey Archipelago study (Olivieret al., 2012). To date, 91 Syllidae species have been recorded in the EC and are established mostly in coarse sediments.
Larval development of Polychaeta from the northern California coast. Fourteen additional species together with seasonality of planktic larvae over a 5-year period
Authors: Blake James A.
Published: August 2017

Larvae of ~100 species of benthic invertebrates were obtained from the vicinity of Tomales Bay and Dillon Beach, California, over a 6-year period (1971–1977). This study reports on larvae of 14 species in eight families of polychaetes:Micronereis nanaimoensis,Nereis vexillosa,Sthenelais fusca,Nephtys caecoides,Nephtys californiensis,Boccardia berkeleyorum,Polydora pygidialis,Polydora spongicola,Dipolydora cardalia,Mediomastus californiensis,Ampharete labrops,Phragmatopoma californica,Sabellaria cementariumandPectinaria californiensis. Some species were cultured from embryos obtained from laboratory fertilizations or field-collected egg masses or capsules. Larvae of other species were obtained from meroplankton and some of these were cultured through metamorphosis. A summary table is presented documenting seasonal occurrence of 60 polychaete taxa in the meroplankton.
Taxonomic revision of Cirratulus (Polychaeta: Cirratulidae) from the coasts of Argentina, with description of a new species
Authors: Saracho Bottero M. Andrea , Elias Rodolfo , Magalhães Wagner F.
Published: August 2017

Among the polychaetes, the family Cirratulidae is one of the most challenging taxonomically because most of the currently used diagnostic characters change during ontogeny. It is therefore necessary to use a combination of characters to make an accurate identification. The present work is the first of a planned revision of the genus Cirratulus from coastal zones of Argentina. The examined material came from the two largest natural history museums of the country, and showed several morphotypes. Some specimens corresponded to described species, but others were undoubtedly undescribed. Among the previously known Cirratulidae, Cirratulus jucundus (Kinberg, 1866) and Cirratulus patagonicus (Kinberg, 1866), formerly distributed for Antarctica are now also present in coastal continental areas of Argentina. The new species Cirratulus mianzanii sp. nov. is described from subtidal areas off Peninsula Valdés, Patagonia. This species is distinguished from its congeners by the segmental origin of both tentacular filaments and first pair of branchiae in the junction between the peristomium and first chaetiger. Branchiae arise some distance from the notopodium but not at the dorsal midline of the body. Ventral groove present along the body, with a fine dark midline. Capillary chaetae are serrate seen under an optical microscope, and the segmental origin of neuro- and notopodial spines is different from other species of the genus.
On the provenance of a historic sledge shoe fragment, said to have been collected by Edward Wilson at the South Pole in 1912
Authors: Rowe Sophie
Published: August 2017

This paper discusses the authentication of a metal sledge shoe fragment, believed by the owner to have been collected by Edward Wilson close to the South Pole on 18 January 1912. Microscopic and elemental analysis show that the object is made from ‘German silver’, a copper alloy used only on Norwegian Nansen-style sledges in the late nineteenth and early twentieth century, and that it was used to clad a tapering sledge runner end about 10 mm thick. By comparing related objects, including sledges used by Amundsen and Scott in their South Pole journeys and a sledge from the Discovery Expedition, we show that the object cannot have come from an English sledge, but would have fitted one of Amundsen's modified sledges. Written sources have been extensively searched, but no direct written provenance for the object exists. However, contemporary Norwegian and British accounts explain specific features of the object and exclude other possible provenances. We conclude that it is most likely that the proposed provenance and history attached to this artefact are correct.
Religious experience and the probability of theism: comments on Swinburne
Authors: JÄGER CHRISTOPH
Published: August 2017

I discuss the role of religious experience in Richard Swinburne's probabilistic case for theism. Swinburne draws on his principle of credulity to argue that, if in addition to other evidence we consider that many people have theistic religious experiences, theism comes out as more probable than not. However, on many plausible probability assignments for the relevant non-experiential evidence, the conditional probability of theism already converges towards 1. Moreover, an argument analogous to a general Bayesian argument against phenomenal conservatism suggests that, after we take account of evidence from religious experience, the probability of theism cannot be greater than the prior probability that the best rival hypothesis is false. I conclude that these observations are compatible with what Swinburne would call ‘weak rational belief’ in theism and that such weak belief can be strong enough for rational faith.
Swinburne's apologetic strategy for theism evaluated
Authors: PHILIPSE HERMAN
Published: August 2017

In this article, I classify Richard Swinburne's apologetic strategy for theism, and raise eight structural problems with regard to his Bayesian approach. For example, is theism really a meaningful theory? Does it have any predictive power? If so, isn't theism a degenerating research programme? Furthermore, is it legitimate to immunize theism against empirical refutations? Is Swinburne's tactic of doing so successful?
The Phantom Public, the Living Newspaper: Reanimating the Public in the Federal Theatre Project's1935(New York, 1936)
Authors: Cox Jordana
Published: August 2017

Stories of American democracy, whether critical or congratulatory, canonical or popular, feature “the public” as their recurring protagonist. “The public” is a rhetorical fixture of political campaigns and democratic theories, opinion polls and calls to action. Its influence is formidable: the very idea scores political speech, and calls citizens into being. Yet as many scholars have argued, “the public” is a moving target, and possibly even a total fiction. Perhaps the best-known challenge in recent decades has come from literary critic and social theorist Michael Warner. “Publics” he writes in hisPublics and Counterpublics,“have become an essential part of the social landscape, and yet it would tax our understanding to say exactly what they are.” If a public is difficult to describe, it is in part, Warner explains, because the idea hovers in modern imaginaries between the concrete and the abstract. “A public” can conjure at once: a bounded audience—“a crowd witnessing itself in visible space”; a more abstract “social totality” like the constituents of a nation; and a community conjured through shared texts or identities.
Third culture kids in the outer circle: The development of sociolinguistic knowledge among local and expatriate children in Singapore
Authors: Starr Rebecca Lurie , Theng Andre Joseph , Wong Kevin Martens , Tong Natalie Jing Yi , Ibrahim Nurul Afiqah Bte , Chua Alicia Mei Yin , Yong Clarice Hui Min , Loke Frances Wei , Dominic Helen , Fernandez Keith Jayden , Peh Matthew Tian Jing
Published: August 2017

Children acquiring sociolinguistic knowledge in transnational migration settings must learn to evaluate multiple languages and dialects in a fluid, multifaceted social landscape. This study examines the sociolinguistic development of local and expatriate children in Singapore and investigates the extent to which they share sociolinguistic knowledge and norms. One hundred fourteen children ages five to nineteen completed a region identification task and an occupation judgment task, focusing on their perception of four regional English varieties: Australian English, Northern-China-accented English, Filipino English, and Singapore English. While all groups performed well on the region identification task, expatriate children outperformed locals within the youngest age group. Singaporean and expatriate children attending local schools showed greater familiarity with local norms than international school students in their occupation ratings. Participants mapped speakers to occupations by general prestige level, suggesting that children rely on indirect knowledge of social status rather than direct experience with speakers in their development of sociolinguistic evaluation. (Children's sociolinguistic development, transnational migration, language attitudes)*
ARE PRODUCT SPREADS USEFUL FOR FORECASTING OIL PRICES? AN EMPIRICAL EVALUATION OF THE VERLEGER HYPOTHESIS
Authors: Baumeister Christiane , Kilian Lutz , Zhou Xiaoqing
Published: August 2017

Many oil industry analysts believe that there is predictive power in the product spread, defined as the difference between suitably weighted refined product market prices and the price of crude oil. We derive a number of alternative forecasting model specifications based on product spreads and compare the implied forecasts to the no-change forecast of the real price of oil. We show that not all product spread models are useful for out-of-sample forecasting, but some models are, even at horizons between one and two years. The most accurate model is a time-varying parameter model of gasoline and heating oil spot price spreads that allows for structural change in product markets. We document mean-squared prediction error reductions as high as 20% and directional accuracy as high as 63% at the two-year horizon, making product spread models a good complement to forecasting models based on economic fundamentals, which work best at short horizons.
FINANCIAL REGULATION AND ENDOGENOUS MACROECONOMIC CRISES
Authors: Riccetti Luca , Russo Alberto , Gallegati Mauro
Published: August 2017

We explore the effects of banking regulation on financial stability and macroeconomic dynamics in an agent-based computational model. In particular, we study the minimum level of capital and the lending concentration towards a single counterpart. We show that an overly tight regulation is dangerous because it reduces credit availability. By contrast, overly loose constraints, associated with a high payout ratio, increase financial fragility that, in turn, damage the real economy. Simulation results support the introduction of regulatory rules aimed at assuring an adequate capitalization of banks, such as the Capital Conservation Buffer (Basel III reform).
ENDOGENOUS GROWTH AND STRUCTURAL CHANGE THROUGH VERTICAL AND HORIZONTAL INNOVATIONS
Authors: Bondarev Anton , Greiner Alfred
Published: August 2017

This paper combines horizontal and vertical innovations to generate an endogenous growth model allowing for structural change as an endogenous phenomenon. Older technologies are continuously replaced by newer ones due to creative destruction, and new technologies appear as a result of horizontal innovations and due to the consumers' preference for variety. We assume fixed operational costs for the manufacturing sector and an endogenously defined patent price for every new technology. Every industry is profitable only for a limited period of time, making the effective time of existence of the technology endogenous and finite. We find that in such an economy endogenous structural change is the source of ongoing economic growth. Furthermore, the range of existing sectors as well as growth rates stays constant as long as the technologies are symmetric.
SUNNY-CP and the MiniZinc challenge
Authors: AMADINI ROBERTO , GABBRIELLI MAURIZIO , MAURO JACOPO
Published: August 2017

In Constraint Programming, a portfolio solver combines a variety of different constraint solvers for solving a given problem. This fairly recent approach enables to significantly boost the performance of single solvers, especially when multicore architectures are exploited. In this work, we give a brief overview of the portfolio solversunny-cp, and we discuss its performance in the MiniZinc Challenge—the annual international competition for Constraint Programming solvers—where it won two gold medals in 2015 and 2016.
Parsing Complex Noun Phrases: Effects of Hierarchical Structure and Sentence Position on Memory Load
Authors: Mota Sergio , Igoa José Manuel
Published: August 2017

In this paper, we report two experiments in Spanish designed to find out what kind of processes underlie the online parsing of complex noun phrases (NPs). To that end, we used a ‘click detection’ paradigm coupled with an oral comprehension task with sentences made up of complex NPs comprising embedded prepositional phrases PPs or coordinate NPs. The critical NPs consisted of words or pseudowords, and were inserted either at subject position (Experiment 1) or at object position (Experiment 2) in the sentence. Results show an opposite pattern of RTs to clicks when the complex NP is located at subject (vs. object) position, with the former case showing heavier processing demands as the parser delves deeper into the complex NP, regardless of the internal constituency of the target NP and its lexical content, and the latter yielding the opposite pattern. These results suggest that structural complexity by itself does not determine an increase in processing costs during sentence parsing, which is only apparent in cases involving deferred operations like subject-verb agreement.
Weed Management Programs with Pyroxasulfone in Field Corn (Zea mays)
Authors: Stephenson Daniel O. , Bond Jason A. , Griffin James L. , Landry Randall L. , Woolam Brandi C. , Edwards H. Matthew , Hardwick John M.
Published: August 2017

Field experiments were conducted in Louisiana and Mississippi from 2011 through 2013 to evaluate crop injury, weed control, and yield in field corn following pyroxasulfone applied PRE and POST. Pyroxasulfone PRE or POST did not injure corn at any evaluation. Barnyardgrass control was not improved with the addition of any POST treatment to pyroxasulfone alone or atrazine plus pyroxasulfone PRE; however, all POST treatments increased barnyardgrass control to at least 95% at all evaluations following atrazine PRE. All treatments that contained a PRE followed by POST application controlled browntop millet ≥90% at all evaluations. All POST treatments increased ivyleaf morningglory control to ≥92% following atrazine or pyroxasulfone alone PRE. However, control with atrazine plus pyroxasulfone PRE was similar or greater 28 d after POST than all treatments that received a POST application. In the absence of a POST treatment, pyroxasulfone or atrazine plus pyroxasulfone PRE controlled Palmer amaranth 93 to 96% at all evaluations, but atrazine alone PRE provided 84, 82, and 66% control 7, 14, and 28 d after POST, respectively. All programs that contained a PRE followed by POST herbicide treatment controlled Palmer amaranth >90% at all evaluations. Corn yield following all treatments except atrazine alone PRE and the nontreated were similar and ranged from 10990 to 12330 kg ha−1. This research demonstrated that pyroxasulfone can be a valuable tool for weed management in a corn weed management program.
p53-R273H upregulates neuropilin-2 to promote cell mobility and tumor metastasis
Authors: Lv Tao , Wu Xianqiang , Sun Lijuan , Hu Qingyong , Wan Yang , Wang Liang , Zhao Zhiqiang , Tu Xiao , Xiao Zhi-Xiong Jim
Published: August 2017

Mounting evidence indicates that hotspot p53 mutant proteins often possess gain-of-function property in promoting cell mobility and tumor metastasis. However, the molecular mechanisms are not totally understood. In this study, we demonstrate that the hotspot mutation, p53-R273H, promotes cell migration, invasion in vitro and tumor metastasis in vivo. p53-R273H significantly represses expression of DLX2, a homeobox protein involved in cell proliferation and pattern formation. We show that p53-R273H-mediated DLX2 repression leads to upregulation of Neuropilin-2 (NRP2), a multifunctional co-receptor involved in tumor initiation, growth, survival and metastasis. p53-R273H-induced cell mobility is effectively suppressed by DLX2 expression. Furthermore, knockdown of NRP2 significantly inhibits p53-R273H-induced tumor metastasis in xenograft mouse model. Together, these results reveal an important role for DLX2-NRP2 in p53-R273H-induced cell mobility and tumor metastasis.
TNFα deficiency results in increased IL-1β in an early onset of spontaneous murine colitis
Authors: De Santis S , Kunde D , Galleggiante V , Liso M , Scandiffio L , Serino G , Pinto A , Campiglia P , Sorrentino R , Cavalcanti E , Santino A , Caruso M L , Eri R , Chieppa M
Published: August 2017

Inflammatory bowel disease (Crohn’s disease (CD) and ulcerative colitis (UC)) is a multifactorial disease resulting from immune dysregulation in the gut. The underlying colitis is characterized by high levels of inflammatory cytokines, including TNFα. Biological intervention for IBD patients using anti-TNFα antibodies is often an effective therapeutic solution. However, TNFα neutralization fails to induce remission in a subgroup of IBD patients, primarily in UC patients. There is a dearth of suitable animal models representing TNFα non-responders. Here we have combined one of the best UC models currently available, namely Winnie and the TNFαKO mouse to generate a TNFα-deficient Winnie to study early onset colitis. The induced TNFα deficiency with underlying colitis does not influence general health (viability and body weight) or clinical parameters (colon weight, colon length and histological colitis) when compared with the Winnie genotype alone. The molecular characterization resulted in identification of Il1β as the major elevated cytokine during early phases of colitis. Further, in vitro functional assay using bone marrow-derived dendritic cells confirmed IL-1β as the major cytokine released in the absence of TNFα. This study has generated a successful model of colitis that remains TNFα non-responsive and has demonstrated that IL-1β expression is a major pathway for the progression of colitis in this system. These data also suggest that IL-1β can be a potential target for clinical intervention of UC patients who fail to respond to TNFα neutralization.
A reversible phospho-switch mediated by ULK1 regulates the activity of autophagy protease ATG4B
Authors: Pengo N. , Agrotis A. , Prak K. , Jones J. , Ketteler R.
Published: August 2017

Upon induction of autophagy, the ubiquitin-like protein LC3 is conjugated to phosphatidylethanolamine (PE) on the inner and outer membrane of autophagosomes to allow cargo selection and autophagosome formation. LC3 undergoes two processing steps, the proteolytic cleavage of pro-LC3 and the de-lipidation of LC3-PE from autophagosomes, both executed by the same cysteine protease ATG4. How ATG4 activity is regulated to co-ordinate these events is currently unknown. Here we find that ULK1, a protein kinase activated at the autophagosome formation site, phosphorylates human ATG4B on serine 316. Phosphorylation at this residue results in inhibition of its catalytic activity in vitro and in vivo. On the other hand, phosphatase PP2A-PP2R3B can remove this inhibitory phosphorylation. We propose that the opposing activities of ULK1-mediated phosphorylation and PP2A-mediated dephosphorylation provide a phospho-switch that regulates the cellular activity of ATG4B to control LC3 processing.
The bHLH transcription factor CgbHLH001 is a potential interaction partner of CDPK in halophyte Chenopodium glaucum
Authors: Wang Juan , Cheng Gang , Wang Cui , He Zhuanzhuan , Lan Xinxin , Zhang Shiyue , Lan Haiyan
Published: August 2017

Plants have evolved different abilities to adapt to the ever-fluctuating environments for sessility. Calcium-dependent protein kinase (CDPK) is believed to play a pivotal role in abiotic stress signaling. So far, study on the specific substrates that CDPK recognized in response to adversity is limited. In the present study, we revealed a potential interaction between CDPK and a bHLH transcription factor under salt stress in Chenopodium glaucum. First, we identified a CgCDPK, which was up-regulated under salt and drought stress; then by Y2H screening, CgCDPK was detected to be involved in interaction with a bHLH TF (named as CgbHLH001), which also positively respond to salt and drought stress. Further computational prediction and experiments including GST-pulldown and BiFC assays revealed that potential interaction existed between CgCDPK and CgbHLH001, and they might interact on the plasma membrane. In addition, CgCDPK-overexpressed transgenic tobacco line could significantly accumulate transcripts of NtbHLH (a homolog of CgbHLH001 in N. tabacum), which provided another evidence of correlation between CgCDPK and CgbHLH001. Our results suggest that CgbHLH001 can interact with CgCDPK in signal transduction pathway in response to abiotic stress, which should provide new evidence for further understanding of the substrate specificity of plant CDPK signaling pathway.
Reputation management promotes strategic adjustment of service quality in cleaner wrasse
Authors: Binning Sandra A. , Rey Olivia , Wismer Sharon , Triki Zegni , Glauser Gaétan , Soares Marta C. , Bshary Redouan
Published: August 2017

Adjusting one’s behaviour in response to eavesdropping bystanders is considered a sophisticated social strategy, yet the underlying mechanisms are not well studied. Cleaner wrasse, Labroides dimidiatus, cooperate by eating ectoparasites off “client” fishes, or cheat (i.e. bite) and eat client mucus. Image scoring by bystander clients generally causes cleaners from socially-complex (i.e. high cleaner and client abundance; high client species richness) habitats to increase levels of cooperation. However, some individuals may periodically provide tactile stimulation to small resident clients, which attract bystanders close that are bitten, a form of tactical deception. Cortisol injection can reproduce this pattern. Here, we tested whether cleaners from socially-complex versus simple habitats respond differently to cortisol injections in terms of their cleaning interactions with clients. We found that only cleaners from the socially-complex habitat respond to cortisol injection with strategies functioning as tactical deception: i.e. increased tactile stimulation to small clients and increased cheating of large clients relative to small ones. At the socially-simple site, where reputation management is less important, cortisol-treated fish increased their overall levels of cheating, especially of small clients. Thus, strategic adjustments to cooperative behaviour and tactical deception are likely context-dependent, forming part of general reputation management abilities in cleaner wrasse.
The 3-D structure of the Somma-Vesuvius volcanic complex (Italy) inferred from new and historic gravimetric data
Authors: Linde Niklas , Ricci Tullio , Baron Ludovic , Shakas Alexis , Berrino Giovanna
Published: August 2017

Existing 3-D density models of the Somma-Vesuvius volcanic complex (SVVC), Italy, largely disagree. Despite the scientific and socioeconomic importance of Vesuvius, there is no reliable 3-D density model of the SVVC. A considerable uncertainty prevails concerning the presence (or absence) of a dense body underlying the Vesuvius crater (1944 eruption) that is implied from extensive seismic investigations. We have acquired relative gravity measurements at 297 stations, including measurements in difficult-to-access areas (e.g., the first-ever measurements in the crater). In agreement with seismic investigations, the simultaneous inversion of these and historic data resolves a high-density body that extends from the surface of the Vesuvius crater down to depths that exceed 2 km. A 1.5-km radius horseshoe-shaped dense feature (open in the southwestern sector) enforces the existing model of groundwater circulation within the SVVC. Based on its volcano-tectonic evolution, we interpret volcanic structures that have never been imaged before.
Erratum: Padé resummation of many-body perturbation theories
Authors: Pavlyukh Y.
Published: August 2017

A correction to this article has been published and is linked from the HTML version of this paper. The error has been fixed in the paper.
Seal Whiskers Vibrate Over Broad Frequencies During Hydrodynamic Tracking
Authors: Murphy Christin T. , Reichmuth Colleen , Eberhardt William C. , Calhoun Benton H. , Mann David A.
Published: August 2017

Although it is known that seals can use their whiskers (vibrissae) to extract relevant information from complex underwater flow fields, the underlying functioning of the system and the signals received by the sensors are poorly understood. Here we show that the vibrations of seal whiskers may provide information about hydrodynamic events and enable the sophisticated wake-tracking abilities of these animals. We developed a miniature accelerometer tag to study seal whisker movement in situ. We tested the ability of the tag to measure vibration in excised whiskers in a flume in response to laminar flow and disturbed flow. We then trained a seal to wear the tag and follow an underwater hydrodynamic trail to measure the whisker signals available to the seal. The results showed that whiskers vibrated at frequencies of 100–300 Hz, with a dynamic response. These measurements are the first to capture the incoming signals received by the vibrissae of a live seal and show that there are prominent signals at frequencies where the seal tactogram shows good sensitivity. Tapping into the mechanoreceptive interface between the animal and the environment may help to decipher the functional basis of this extraordinary hydrodynamic detection ability.
The trap bag concept of afterglow luminescence
Authors: Huang Yih-Ping , Lin Jeng-Wen
Published: August 2017

This article explains the behavior of afterglow luminescence using the trap bag concept, in which a constant phosphor dose contains a presumed bag with the ability to capture or release electrons through its opening. Luminescence is emitted as the bag releases the captured electrons. The electron-holding capacity is determined by the irradiation conditions, the width of the opening, and the electron activation; these factors are inherent properties of the long persistent luminescence (PLUM) dose and are affected by the thermal status. During the afterglow stage, higher temperatures may result in a wider opening and increased activation of electrons released from the bag, thus creating a higher light intensity and leading to the quicker exhaustion of the electrons within. In contrast, the opposite phenomenon will occur at lower temperatures. This article provides a detailed explanation of the trap bag concept at various thermal statuses and provides a method for delaying the afterglow peak profile through temperature change. Experimental tests were performed to confirm the proposed concept.
Measuring and modelling the response of Klebsiella pneumoniae KPC prey to Bdellovibrio bacteriovorus predation, in human serum and defined buffer
Authors: Baker Michelle , Negus David , Raghunathan Dhaarini , Radford Paul , Moore Chris , Clark Gemma , Diggle Mathew , Tyson Jess , Twycross Jamie , Sockett R. Elizabeth
Published: August 2017

In worldwide conditions of increasingly antibiotic-resistant hospital infections, it is important to research alternative therapies. Bdellovibrio bacteriovorus bacteria naturally prey on Gram-negative pathogens, including antibiotic-resistant strains and so B. bacteriovorus have been proposed as “living antibiotics” to combat antimicrobially-resistant pathogens. Predator-prey interactions are complex and can be altered by environmental components. To be effective B. bacteriovorus predation needs to work in human body fluids such as serum where predation dynamics may differ to that studied in laboratory media. Here we combine mathematical modelling and lab experimentation to investigate the predation of an important carbapenem-resistant human pathogen, Klebsiella pneumoniae, by B. bacteriovorus in human serum versus buffer. We show experimentally that B. bacteriovorus is able to reduce prey numbers in each environment, on different timescales. Our mathematical model captures the underlying dynamics of the experimentation, including an initial predation-delay at the predator-prey-serum interface. Our research shows differences between predation in buffer and serum and highlights both the potential and limitations of B. bacteriovorus acting therapeutically against K. pneumoniae in serum, informing future research into the medicinal behaviours and dosing of this living antibacterial.
Biogeographic distributions of neotropical trees reflect their directly measured drought tolerances
Authors: Esquivel-Muelbert Adriane , Galbraith David , Dexter Kyle G. , Baker Timothy R. , Lewis Simon L. , Meir Patrick , Rowland Lucy , Costa Antonio Carlos Lola da , Nepstad Daniel , Phillips Oliver L.
Published: August 2017

High levels of species diversity hamper current understanding of how tropical forests may respond to environmental change. In the tropics, water availability is a leading driver of the diversity and distribution of tree species, suggesting that many tropical taxa may be physiologically incapable of tolerating dry conditions, and that their distributions along moisture gradients can be used to predict their drought tolerance. While this hypothesis has been explored at local and regional scales, large continental-scale tests are lacking. We investigate whether the relationship between drought-induced mortality and distributions holds continentally by relating experimental and observational data of drought-induced mortality across the Neotropics to the large-scale bioclimatic distributions of 115 tree genera. Across the different experiments, genera affiliated to wetter climatic regimes show higher drought-induced mortality than dry-affiliated ones, even after controlling for phylogenetic relationships. This pattern is stronger for adult trees than for saplings or seedlings, suggesting that the environmental filters exerted by drought impact adult tree survival most strongly. Overall, our analysis of experimental, observational, and bioclimatic data across neotropical forests suggests that increasing moisture-stress is indeed likely to drive significant changes in floristic composition.
Nitric Oxide regulates mouth development in amphioxus
Authors: Annona Giovanni , Caccavale Filomena , Pascual-Anaya Juan , Kuratani Shigeru , De Luca Pasquale , Palumbo Anna , D’Aniello Salvatore
Published: August 2017

The development of the mouth in animals has fascinated researchers for decades, and a recent study proposed the modern view of recurrent evolution of protostomy and deuterostomy. Here we expanded our knowledge about conserved traits of mouth formation in chordates, testing the hypothesis that nitric oxide (NO) is a potential regulator of this process. In the present work we show for the first time that NO is an essential cell signaling molecule for cephalochordate mouth formation, as previously shown for vertebrates, indicating its conserved ancestral role in chordates. The experimental decrease of NO during early amphioxus Branchiostoma lanceolatum development impaired the formation of the mouth and gill slits, demonstrating that it is a prerequisite in pharyngeal morphogenesis. Our results represent the first step in the understanding of NO physiology in non-vertebrate chordates, opening new evolutionary perspectives into the ancestral importance of NO homeostasis and acquisition of novel biological roles during evolution.
A biomechanical approach to understand the ecomorphological relationship between primate mandibles and diet
Authors: Marcé-Nogué Jordi , Püschel Thomas A. , Kaiser Thomas M.
Published: August 2017

The relationship between primate mandibular form and diet has been previously analysed by applying a wide array of techniques and approaches. Nonetheless, most of these studies compared few species and/or infrequently aimed to elucidate function based on an explicit biomechanical framework. In this study, we generated and analysed 31 Finite Element planar models of different primate jaws under different loading scenarios (incisive, canine, premolar and molar bites) to test the hypothesis that there are significant differences in mandibular biomechanical performance due to food categories and/or food hardness. The obtained stress values show that in primates, hard food eaters have stiffer mandibles when compared to those that rely on softer diets. In addition, we find that folivores species have the weakest jaws, whilst omnivores have the strongest mandibles within the order Primates. These results are highly relevant because they show that there is a strong association between mandibular biomechanical performance, mandibular form, food hardness and diet categories and that these associations can be studied using biomechanical techniques rather than focusing solely on morphology.
Physical implementation of oblivious transfer using optical correlated randomness
Authors: Ito Tomohiro , Koizumi Hayato , Suzuki Nobumitsu , Kakesu Izumi , Iwakawa Kento , Uchida Atsushi , Koshiba Takeshi , Muramatsu Jun , Yoshimura Kazuyuki , Inubushi Masanobu , Davis Peter
Published: August 2017

We demonstrate physical implementation of information-theoretic secure oblivious transfer based on bounded observability using optical correlated randomness in semiconductor lasers driven by common random light broadcast over optical fibers. We demonstrate that the scheme can achieve one-out-of-two oblivious transfer with effective key generation rate of 110 kb/s. The results show that this scheme is a promising approach to achieve information-theoretic secure oblivious transfer over long distances for future applications of secure computation such as privacy-preserving database mining, auctions and electronic-voting.
Spatial Regression and Prediction of Water Quality in a Watershed with Complex Pollution Sources
Authors: Yang Xiaoying , Liu Qun , Luo Xingzhang , Zheng Zheng
Published: August 2017

Fast economic development, burgeoning population growth, and rapid urbanization have led to complex pollution sources contributing to water quality deterioration simultaneously in many developing countries including China. This paper explored the use of spatial regression to evaluate the impacts of watershed characteristics on ambient total nitrogen (TN) concentration in a heavily polluted watershed and make predictions across the region. Regression results have confirmed the substantial impact on TN concentration by a variety of point and non-point pollution sources. In addition, spatial regression has yielded better performance than ordinary regression in predicting TN concentrations. Due to its best performance in cross-validation, the river distance based spatial regression model was used to predict TN concentrations across the watershed. The prediction results have revealed a distinct pattern in the spatial distribution of TN concentrations and identified three critical sub-regions in priority for reducing TN loads. Our study results have indicated that spatial regression could potentially serve as an effective tool to facilitate water pollution control in watersheds under diverse physical and socio-economical conditions.
Absolute measurement of gene transcripts with Selfie-digital PCR
Authors: Podlesniy Petar , Trullas Ramon
Published: August 2017

Absolute measurement of the number of RNA transcripts per gene is necessary to compare gene transcription among different tissues or experimental conditions and to assess transcription of genes that have a variable copy number per cell such as mitochondrial DNA. Here, we present a method called Selfie-digital PCR that measures the absolute amount of an RNA transcript produced by its own coding DNA at a particular moment. Overcoming the limitations of previous approaches, Selfie-digital PCR allows for the quantification of nuclear and mitochondrial gene transcription in a strand-specific manner that is comparable among tissues and cell types that differ in gene copy number or metabolic state. Using Selfie-digital PCR, we found that, with the exception of the liver, different organs exhibit marked variations in mitochondrial DNA copy number but similar transcription of mitochondrial DNA heavy and light chains, thus suggesting a preferential role of mitochondrial DNA abundance over its transcription in organ function. Moreover, the strand-specific analysis of mitochondrial transcription afforded by Selfie-digital PCR showed that transcription of the heavy strand was significantly higher than that of the light strand in all the tissues studied.
Template-Assisted Preparation of Micrometric Suspended Membrane Lattices of Photoluminescent and Non-Photoluminescent Polymers by Capillarity-Driven Solvent Evaporation: Application to Microtagging
Authors: Polito Giovanni , Robbiano Valentina , Cozzi Chiara , Cacialli Franco , Barillaro Giuseppe
Published: August 2017

In this work, the bottom-up template-assisted preparation of high-density lattices (up to 11 · 106 membranes/cm2) of suspended polymer membranes with micrometric size (in the order of few μm2) and sub-micrometric thickness (in the order of hundreds of nm) is demonstrated for both photoluminescent and non-photoluminescent polymers by capillarity-driven solvent evaporation. Solvent evaporation of low concentration polymer solutions drop-cast on an array of open-ended micropipes is shown to lead to polymer membrane formation at the inlet of the micropipes thanks to capillarity. The method is proven to be robust with high-yield (>98%) over large areas (1 cm2) and of general validity for both conjugated and non-conjugated polymers, e.g. poly(9,9-di-n-octylfluorene-alt-benzothiadiazole (F8BT), poly[2-methoxy-5-(3′,7′-dimethyloctyloxy)-1,4-phenylenevinylene] (MDMO-PPV), polystyrene (PS), thus breaking a new ground on the controlled preparation of polymer micro and nanostructures. Angle dependence and thermal stability of photoluminescence emission arising from F8BT membrane lattices was thorough investigated, highlighting a non-Lambertian photoluminescence emission of membrane lattices with respect to F8BT films. The method is eventually successfully applied to the preparation of both photoluminescent and non-photoluminescent micro Quick Response (μQR) codes using different polymers, i.e. F8BT, MDMO-PPV, PS, thus providing micrometric-sized taggants suitable for anti-counterfeiting applications.