bluuebunny/crossref_metadata_2025 huggingface dataset:
Free Radical-Scavenging, Anti-Inflammatory, and Antibacterial Activities of Water and Ethanol Extracts Prepared from Compressional-Puffing Pretreated Mango (Mangifera indicaL.) Peels
Authors: Huang Chun-Yung , Kuo Chia-Hung , Wu Chien-Hui , Kuan Ai-Wei , Guo Hui-Ru , Lin Yu-Hua , Wang Po-Kai
Published: February 2018

During the processing of mango, a huge amount of peel is generated, which is environmentally problematic. In the present study, a compressional-puffing process was adopted to pretreat the peels of various mango cultivars, and then the bioactive compounds of mango peels were extracted by water or ethanol. The phenolic compound compositions as well as the free radical-scavenging, anti-inflammatory, and antibacterial activities of water extract (WE) and ethanol extract (EE) from nonpuffed (NP) and compressional-puffed (CP) mango peels were further evaluated. It was found that compressional-puffing could increase the yield of extracts obtained from most mango varieties and could augment the polyphenol content of extracts from Jinhwang and Tainoung number 1 (TN1) cultivars. The WE and EE from TN1 exhibited the highest polyphenol content and the greatest free radical-scavenging activities among the mango cultivars tested. Seven phenolic compounds (gallic acid, pyrogallol, chlorogenic acid,p-hydroxybenzoic acid,p-coumaric acid, ECG, and CG) were detected in CPWE (compressional-puffed water extract) and CPEE (compressional-puffed ethanol extract) from TN1, and antioxidant stability of both CPWE and CPEE was higher than that of vitamin C. Further biological experiments revealed that CPEE from TN1 possessed the strongest anti-inflammatory and antibacterial activities, and thus it is recommended as a multibioactive agent, which may have applications in the food, cosmetic, and nutraceutical industries.
Perspective of Family Members of Transitions to Alternative Levels of Care in Anglo-Saxon Countries
Authors: Merla C. , Wickson-Griffiths A. , Kaasalainen S. , Dal Bello-Haas V. , Banfield L. , Hadjistavropoulos T. , Di Sante E.
Published: February 2018

This scoping review explores circumstances surrounding the decision about, and eventual experience of, transitioning older adults into alternative levels of housing (ALH), such as long-term care. This topic is examined from a family member perspective, given their exposure and involvement in the care of older adult relatives during this transitional period. The scoping review methodology is based on the framework of Arksey and O’Malley and subsequent recommendations from Levac, Colquhoun, and O’Brien. Approximately 470 articles were reviewed covering the period between 2000 and November 2014; 37 articles met inclusion criteria. A temporal organization of themes was used to describe the experiences of family members in the pretransition, active transition, and posttransition periods of moving older adult relatives into ALH. This paper highlights the transitional period as a time of crisis, with a lack of planning, support, and transparent discussion. This study identifies a need for future research on the potential benefits of family support groups, interim transitional housing options, different models of ALH, changing roles in the posttransition period, and the need for a comprehensive list of housing options for older adults. Results have the potential to inform policy/practice and improve the lives of older adults and their family.
Analysis Model of Cost-Effectiveness for Value Evaluation of Building Elements
Authors: Lee Jongsik
Published: February 2018

As various building materials have been developed, the combination of materials that make up the building elements has also increased exponentially. The materials making up the elements of the building will affect the performance of the building and the LCC. In order to improve the value of buildings in Korea, value engineering has been mandated in public construction projects with a project cost of over 10 billion won since 2000. The value index for systems (materials, elements, facilities, etc.) constituting buildings is calculated. However, the method for calculating the value index has not yet been normalized. The performance evaluation of the building systems (materials, elements, facilities, etc.) used in the current work and the method of calculating the value index for converting the LCC into a grade may vary depending on how the range of the grade is set. Even if the objects being evaluated are the same, there arises a problem that the results change depending on the value evaluation method. Therefore, this study tried to develop a value evaluation method that could draw consistent value evaluation results. For this purpose, this study presents a cost-effectiveness analysis model for the physical performance of the building elements and the value evaluation of LCC. Since the various physical performances of the building elements have different properties, normalization is required for comparison of physical performance values. In order to normalize the LCC and the 14 different physical performances of the building elements, a numerical model was designed using a linear transformation method and a vector normalization method. The cost-effectiveness analysis model proposed in this study was applied to two types of floor elements applicable to apartments in Korea, in order to evaluate the value and verify the consistency of this study’s model. The cost-effectiveness analysis model proposed in this study can help to derive reliable results when it comes to value evaluation for various existing building element compositions.
Metformin Regulating miR-34a Pathway to Inhibit Egr1 in Rat Mesangial Cells Cultured with High Glucose
Authors: Wu Can , Qin Ningning , Ren Huiwen , Yang Min , Liu Shuang , Wang Qiuyue
Published: February 2018

Background. Activating AMPKα negatively regulates Egr1 to inhibit inflammatory cytokines in high glucose. miR-34a inhibition increases phosphorylated AMPKα through mediating SIRT1 to suppress the development of fatty liver. Aim of the Study. To clarify the function of Egr1 on the inflammation and fibrosis in high glucose-cultured MCs, as well as to explore the effects of metformin on miR-34a pathway and Egr1 expression. Methods. We transfected MCs with miR-34a inhibitor. And MCs were transfected with small interfering RNA for silencing Egr1 and SIRT1. Quantitative real-time PCR was used to assay the transcription levels of Egr1 mRNA and miR-34a. Western blot was used to test the protein. And ELISA was used to measure inflammatory factors. Results. High glucose upregulates Egr1 to aggravate the inflammation and fibrosis in MCs. miR-34a suppresses the activation of SIRT1/AMPKα and results in promoting Egr1 in high glucose-cultured MCs. Metformin attenuates high glucose-stimulated inflammation and fibrosis in MCs by regulating miR-34a-mediated SIRT1/AMPKα activity and the downstream Egr1 protein. Conclusion. We enriched the effects of miR-34a pathway regulating Egr1 in high glucose-cultured MCs. It provides a foundation for future researches considering Egr1 as a therapeutic target and a new direction for the clinical application of metformin in early DKD.
Bartter Syndrome Type 1 Presenting as Nephrogenic Diabetes Insipidus
Authors: Vergine Gianluca , Fabbri Elena , Pedini Annalisa , Tedeschi Silvana , Borsa Niccolò
Published: February 2018

Bartter syndrome (BS) type 1 (OMIM #601678) is a hereditary salt-losing renal tubular disorder characterized by hypokalemic metabolic alkalosis, hypercalciuria, nephrocalcinosis, polyuria, recurrent vomiting, and growth retardation. It is caused by loss-of-function mutations of the SLC12A1 gene, encoding the furosemide-sensitive Na-K-Cl cotransporter. Recently, a phenotypic variability has been observed in patients with genetically determined BS, including absence of nephrocalcinosis, hypokalemia, and/or metabolic alkalosis in the first year of life as well as persistent metabolic acidosis mimicking distal renal tubular acidosis. We report the case of a child with a genetically determined diagnosis of Bartter syndrome type 1 who presented with a phenotype of nephrogenic diabetes insipidus, with severe hypernatremia and urinary concentrating defect. In these atypical cases, molecular analysis is mandatory to define the diagnosis, in order to establish the correct clinical and therapeutic management.
A Dynamic Programming-Based Sustainable Inventory-Allocation Planning Problem with Carbon Emissions and Defective Item Disposal under a Fuzzy Random Environment
Authors: Kang Kai , Pu Wei , Ma Yanfang
Published: February 2018

There is a growing concern that business enterprises focus primarily on their economic activities and ignore the impact of these activities on the environment and the society. This paper investigates a novel sustainable inventory-allocation planning model with carbon emissions and defective item disposal over multiple periods under a fuzzy random environment. In this paper, a carbon credit price and a carbon cap are proposed to demonstrate the effect of carbon emissions’ costs on the inventory-allocation network costs. The percentage of poor quality products from manufacturers that need to be rejected is assumed to be fuzzy random. Because of the complexity of the model, dynamic programming-based particle swarm optimization with multiple social learning structures, a DP-based GLNPSO, and a fuzzy random simulation are proposed to solve the model. A case is then given to demonstrate the efficiency and effectiveness of the proposed model and the DP-based GLNPSO algorithm. The results found that total costs across the inventory-allocation network varied with changes in the carbon cap and that carbon emissions’ reductions could be utilized to gain greater profits.
Bronchogenic Cyst as an Unusual Cause of a Persistent Cough and Wheeze in Children: A Case Report and Literature Review
Authors: Abushahin Ahmed , Zarroug Abdulla , Wagdi Magda , Janahi Ibrahim
Published: February 2018

Wheezing and cough are common case scenarios that pediatricians encountered in their office practices. Although a bronchogenic cyst is an uncommon condition, it is essential to be considered in the differential diagnosis of a chronic cough and wheezing among young children who fail to respond to appropriate medical treatment. A 28-month-old girl was referred to our pediatric pulmonology clinic with persistent symptoms of a cough and wheeze unresponsive to standard asthma therapy. This presentation prompted us to undertake a detailed diagnostic evaluation. The evaluation exposed a cystic mass in the middle mediastinum compressing the trachea and left main bronchus. The cyst was excised and confirmed pathologically to be a benign bronchogenic cyst. Subsequently, the patient recovered well and had been free of respiratory symptoms during follow-up visits. This report highlights one of the rare causes of wheezing and cough in young children and emphasizes the importance of considering it in the differential diagnosis of a child presenting with refractory asthma-like symptoms. This is important for early diagnosis and management and to avoid unpredictable complications of this treatable condition.
Congestion Control and Traffic Scheduling for Collaborative Crowdsourcing in SDN Enabled Mobile Wireless Networks
Authors: Shen Dawei , Yan Wei , Peng Yuhuai , Fu Yanhua , Deng Qingxu
Published: February 2018

Currently, a number of crowdsourcing‐based mobile applications have been implemented in mobile networks and Internet of Things (IoT), targeted at real‐time services and recommendation. The frequent information exchanges and data transmissions in collaborative crowdsourcing are heavily injected into the current communication networks, which poses great challenges for Mobile Wireless Networks (MWN). This paper focuses on the traffic scheduling and load balancing problem in software‐defined MWN and designs a hybrid routing forwarding scheme as well as a congestion control algorithm to achieve the feasible solution. The traffic scheduling algorithm first sorts the tasks in an ascending order depending on the amount of tasks and then solves it using a greedy scheme. In the proposed congestion control scheme, the traffic assignment is first transformed into a multiknapsack problem, and then the Artificial Fish Swarm Algorithm (AFSA) is utilized to solve this problem. Numerical results on practical network topology reveal that, compared with the traditional schemes, the proposed congestion control and traffic scheduling schemes can achieve load balancing, reduce the probability of network congestion, and improve the network throughput.
Exogenous β-Hydroxybutyrate Treatment and Neuroprotection in a Suckling Rat Model of Hypoxic-Ischemic Encephalopathy
Authors: Lee Byong Sop , Woo Dong-Cheol , Woo Chul-Woong , Kim Ki-Soo
Published: February 2018

β-Hydroxybutyrate (BHB) is a representative ketone body that may play a role in the mitigation of neonatal hypoxic-ischemic encephalopathy by altering energy metabolism. This study aimed to investigate the neuroprotective efficacy of exogenous BHB administration in a suckling rat model after hypoxia-ischemia (HI). Thirteen-day-old (P13) rat pups were subjected to 120 min of hypoxia according to the Rice-Vannucci model. BHB (5.0 mmol/kg, HI-BHB) or vehicle (0.9% saline, HI-Veh) was administered 0, 2, 4, and 6 h after HI induction. Pathologic injury scores and the number of TUNEL-positive cells were evaluated on P15. Residual hemispheric volume was measured with T2-weighted MRI (on P27) and functional tests, such as the negative geotaxis test, rope suspension test, rotarod test, novel object recognition test, and cylinder test, were performed. Systemic ketosis (approx. 2.0–3.0 mM/L) was well tolerated by the rat pups with no difference in the mortality rate between both groups. Compared with the HI-Veh group, the HI-BHB group demonstrated significantly lower pathological scores as well as fewer TUNEL-positive cells. The intact residual hemispheric and hippocampal volumes were greater in the HI-BHB group than the HI-Veh group. However, the results of functional tests did not differ between both groups. Postischemic BHB administration reduced brain injury in suckling rats after HI. The safe clinical application of our animal model to human infants with HI requires further investigation.
Clinical, Hormonal, and Genetic Evaluation of Idiopathic Nonobstructive Azoospermia and Klinefelter Syndrome Patients
Authors: Kim Shin Y. , Lee Bom Y. , Oh Ah R. , Park So Y. , Lee Hyo S. , Seo Ju T.
Published: February 2018

To investigate the clinical, hormonal, and genetic factors in infertile men with idiopathic nonobstructive azoospermia (NOA) or azoospermic Klinefelter syndrome (KFS), a total of 556 and 96 patients, respectively, were included in this study. All patient samples were analyzed cytogenetically. Serum reproductive hormone levels were measured. Microdeletions in the azoospermia factor (AZF) region of the Y chromosome were detected by multiplex PCR using 16 specific sequence-tagged sites. FSH and LH levels in both NOA and KFS patients were significantly higher than the normal range, and the testosterone level in KFS patients was significantly lower. Ninety-two (95.8%) of the KFS patients showed non-mosaic 47,XXY karyotypes and 47,XXY,inv(9)(p11.1q13); the other KFS patients had mosaic karyotypes of 47,XXY/46,XY, 47,XXY/46,XX, and 47,XXY/48,XXXY/46,XX. Among the 556 idiopathic NOA patients with normal karyotypes, 67 (12.05%) had microdeletions in the AZF region of the Y chromosome. Microdeletions were most frequently detected in the AZFc region, followed by AZFa, AZFb, AZFbc, and partial AZFc deletions. However, Y chromosome microdeletions were not found in any of the azoospermic KFS patients. In view of the hormonal and genetic abnormalities in infertile men with idiopathic NOA and with azoospermic KFS, genetic testing for karyotype, Y chromosome microdeletions, and hormonal parameters is advocated.
The Association Between Oxidative Stress Alleviation via Sulforaphane-Induced Nrf2-HO-1/NQO-1 Signaling Pathway Activation and Chronic Renal Allograft Dysfunction Improvement
Authors: Lv Daoyuan , Zhou Qin , Xia Yue , You Xu , Zhao Zhihong , Li Yongqiang , Zou Hequn
Published: February 2018

Background/Aims: Chronic renal allograft dysfunction (CRAD) is a leading cause of long-term renal allograft loss. Oxidative stress may account for the nonspecific interstitial fibrosis and tubular atrophy that occur in CRAD. An antioxidant intervention via Nrf2 signaling pathway activation might be a promising therapy for some kidney diseases. The present paper investigates whether there is an association between oxidative stress alleviation via sulforaphane-induced Nrf2-HO-1/NQO-1 signaling pathway activation and CRAD improvement. Methods: F344 rat kidneys were orthotopically transplanted into Lewis rat recipients to establish CRAD models. Sulforaphane was administered at 1.5 mg/kg intraperitoneally once daily. Renal function and 24-hour urinary protein were monitored for variations for 24 weeks after transplantation. After 24 weeks, renal histopathology was evaluated according to the Banff criteria after hematoxylin and eosin, Masson’s trichrome and periodic acid-Schiff stainings. Additionally, intrarenal oxidative stress was assessed by the indicators malondialdehyde, 8-isoprostane, oxidized-low density lipoprotein and 8-hydroxy-2’-deoxyguanosine, as well as the activity levels of the antioxidant enzymes total superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and γ-glutamylcysteine synthetase. Nrf2, HO-1 and NQO-1 expression levels were determined via immunohistochemical and Western blot analyses. Results: The sulforaphane-induced Nrf2-HO-1/NQO-1 signaling pathway activation, as demonstrated by immunohistochemical and Western blot analyses, delayed the progression of serum creatinine and blood urea nitrogen, particularly lowering the 24-hour urinary protein levels of CRAD. The semi-quantified histopathological changes were also alleviated. Evidence of oxidative stress alleviation, as indicated by a concurrent decrease in the indicators and sustained levels of antioxidant enzymes activity, was found in the renal allografts after sulforaphane intervention. Conclusion: Oxidative stress alleviation caused by continuous sulforaphane-induced Nrf2-HO-1/NQO-1 signaling pathway activation is associated with functional and morphological improvements of CRAD.
A Novel Method to Detect Early Colorectal Cancer Based on Chromosome Copy Number Variation in Plasma
Authors: Xu Jun-Feng , Kang Qian , Ma Xing-Yong , Pan Yuan-Ming , Yang Lang , Jin Peng , Wang Xin , Li Chen-Guang , Chen Xiao-Chen , Wu Chao , Jiao Shao-Zhuo , Sheng Jian-Qiu
Published: February 2018

Background/Aims: Colonoscopy screening has been accepted broadly to evaluate the risk and incidence of colorectal cancer (CRC) during health examination in outpatients. However, the intrusiveness, complexity and discomfort of colonoscopy may limit its application and the compliance of patients. Thus, more reliable and convenient diagnostic methods are necessary for CRC screening. Genome instability, especially copy-number variation (CNV), is a hallmark of cancer and has been proved to have potential in clinical application. Methods: We determined the diagnostic potential of chromosomal CNV at the arm level by whole-genome sequencing of CRC plasma samples (n = 32) and healthy controls (n = 38). Arm level CNV was determined and the consistence of arm-level CNV between plasma and tissue was further analyzed. Two methods including regular z score and trained Support Vector Machine (SVM) classifier were applied for detection of colorectal cancer. Results: In plasma samples of CRC patients, the most frequent deletions were detected on chromosomes 6, 8p, 14q and 1p, and the most frequent amplifications occurred on chromosome 19, 5, 2, 9p and 20p. These arm-level alterations detected in plasma were also observed in tumor tissues. We showed that the specificity of regular z score analysis for the detection of colorectal cancer was 86.8% (33/38), whereas its sensitivity was only 56.3% (18/32). Applying a trained SVM classifier (n = 40 in trained group) as the standard to detect colorectal cancer relevance ratio in the test samples (n = 30), a sensitivity of 91.7% (11/12) and a specificity 88.9% (16/18) were finally reached. Furthermore, all five early CRC patients in stages I and II were successfully detected. Conclusion: Trained SVM classifier based on arm-level CNVs can be used as a promising method to screen early-stage CRC.
A Strategy for Optimizing the Combination of Active Components Based on Chinese Medicinal Formula Sheng-Mai-San for Myocardial Ischemia
Authors: Li Fang , Fan Xiao-Xue , Chu Chun , Zhang Yu , Kou Jun-Ping , Yu Bo-Yang
Published: February 2018

Background/Aims: Traditional Chinese medicine (TCM) has been used in clinical practice for thousands of years and has accumulated considerable knowledge concerning the in vivo efficacy of targeting complicated diseases. TCM formulae are a mixture of hundreds of chemical components with multiple potential targets, essentially acting as a combination therapy of multi-component drugs. However, the obscure substances and the unclear molecular mechanisms are obstacles to their further development and internationalization. Therefore, it is necessary to develop new modern drugs based on the combination of effective components in TCM with exact clinical efficacy. In present study, we aimed to detect optimal ratio of the combination of effective components based on Sheng-Mai-San for myocardial ischemia. Methods: On the basis of preliminary studies and references of relevant literature about Sheng-Mai-San for myocardial ischemia, we chose three representative components (ginsenoside Rb1 (G), ruscogenin (R) and schisandrin (S)) for the optimization design studies. First, the proper proportion of the combination was explored in different myocardial ischemia mice induced by isoproterenol and pituitrin based on orthogonal design. Then, the different proportion combinations were further optimized through uniform design in a multi-model and multi-index mode. Finally, the protective effect of combination was verified in three models of myocardial ischemia injured by ischemia/reperfusion, chronic intermittent hypoxia and acute infarction. Results: The optimized combination GRS (G: 6 mg/kg, R: 0.75 mg/kg, S: 6 mg/kg) obtained by experimental screening exhibited a significant protective effect on myocardial ischemia injury, as evidenced by decreased myocardium infarct size, ameliorated histological features, decreased myocardial myeloperoxidase (MPO) and malondiadehyde (MDA), calcium overload, and decreased serum lactate dehydrogenase (LDH), creatine kinase MB isoenzyme (CK-MB), cardiac troponin I (cTn-I) activity. In addition, the interactions of three components in combination GRS were also investigated. The combination, compared to G, R and S, could significantly reduce the concentration of serum CK-MB and cTn-I, and decrease myocardial infarct size, which demonstrated the advantages of this combination for myocardial ischemia. Conclusion: Our results demonstrated that the optimized combination GRS could exert significant cardioprotection against myocardial ischemia injury with similar effect compared to Sheng Mai preparations, which might provide some pharmacological evidences for further development of new modern Chinese drug for cardiovascular diseases basing on traditional Chinese formula with affirmative therapeutic effect.
Metformin-Induced Changes of the Coding Transcriptome and Non-Coding RNAs in the Livers of Non-Alcoholic Fatty Liver Disease Mice
Authors: Guo Jun , Zhou Yuan , Cheng Yafen , Fang Weiwei , Hu Gang , Wei Jie , Lin Yajun , Man Yong , Guo Lixin , Sun Mingxiao , Cui Qinghua , Li Jian
Published: February 2018

Background/Aims: Recent studies have suggested that changes in non-coding mRNA play a key role in the progression of non-alcoholic fatty liver disease (NAFLD). Metformin is now recommended and effective for the treatment of NAFLD. We hope the current analyses of the non-coding mRNA transcriptome will provide a better presentation of the potential roles of mRNAs and long non-coding RNAs (lncRNAs) that underlie NAFLD and metformin intervention. Methods: The present study mainly analysed changes in the coding transcriptome and non-coding RNAs after the application of a five-week metformin intervention. Liver samples from three groups of mice were harvested for transcriptome profiling, which covered mRNA, lncRNA, microRNA (miRNA) and circular RNA (circRNA), using a microarray technique. Results: A systematic alleviation of high-fat diet (HFD)-induced transcriptome alterations by metformin was observed. The metformin treatment largely reversed the correlations with diabetes-related pathways. Our analysis also suggested interaction networks between differentially expressed lncRNAs and known hepatic disease genes and interactions between circRNA and their disease-related miRNA partners. Eight HFD-responsive lncRNAs and three metformin-responsive lncRNAs were noted due to their widespread associations with disease genes. Moreover, seven miRNAs that interacted with multiple differentially expressed circRNAs were highlighted because they were likely to be associated with metabolic or liver diseases. Conclusions: The present study identified novel changes in the coding transcriptome and non-coding RNAs in the livers of NAFLD mice after metformin treatment that might shed light on the underlying mechanism by which metformin impedes the progression of NAFLD.
Device Thrombosis After Percutaneous Left Atrial Appendage Occlusion Is Related to Patient and Procedural Characteristics but Not to Duration of Postimplantation Dual Antiplatelet Therapy
Authors: Pracon Radoslaw , Bangalore Sripal , Dzielinska Zofia , Konka Marek , Kepka Cezary , Kruk Mariusz , Kaczmarska-Dyrda Edyta , Petryka-Mazurkiewicz Joanna , Bujak Sebastian , Solecki Mateusz , Pskit Agnieszka , Dabrowska Agnieszka , Sieradzki Bartosz , Plonski Andrzej , Ruzyllo Witold , Witkowski Adam , Demkow Marcin
Published: February 2018

Background—

Device-related thrombus (DRT) after left atrial appendage occlusion is a worrisome finding with little knowledge about when to expect it and how to prevent it. This study sought to investigate correlates of DRT after left atrial appendage occlusion, its time of diagnosis, and particularly, association with postimplantation dual antiplatelet therapy duration.

Methods and Results—

Consecutive patients (n=102) after left atrial appendage occlusion with AMPLATZER Cardiac Plug/Amulet (n=59) or WATCHMAN (n=43) were included in a prospective registry (October 2011–May 2016). Follow-up was done at 1.5, 3 to 6, and 12 months postimplantation. DRT was classified as early (at 1.5 month), late (at 3–6 month), or very late (at 12-month follow-up). Postimplantation dual antiplatelet therapy was recommended for 30 to 180 days and decided independently by attending physicians. Final analysis included 99 patients, 42 (42.4%) females, with median CHA
2
DS
2
-VASc of 4.0 (interquartile range [IQR], 3.0–5.0) and median HAS-BLED score of 2.0 (IQR, 1.0–3.0). DRTs were observed in 7 (7.1%) patients: 2 (28.6%) early, 2 (28.6%) late, and 3 (42.9%) very late. When compared with patients without DRT, those with DRT presented more often with a history of prior thromboembolism (5 [71.4%] versus 28 [30.4%];
P
=0.04), had lower left ventricular ejection fraction (50.0 [IQR, 35.0–55.0] versus 60.0 [IQR, 55.0–66.0];
P
<0.01), and had greater proportion of patients with deep device implantation (6 [85.7%] versus 36 [39.1%];
P
=0.04) and with larger devices implanted (30.0 mm [IQR, 27.0–33.0] versus 25.0 mm [IQR, 24.0–28.0];
P
<0.01). Postimplantation dual antiplatelet therapy duration was not different between the 2 groups (12.4 weeks [IQR, 6.0–49.7] with DRT versus 13.0 weeks [IQR, 7.3–26.0] without DRT;
P
=0.77).

Conclusions—

In this real-world series, DRT was observed early, late, and very late after left atrial appendage occlusion. It was related to patient and procedural characteristics but not to postimplantation dual antiplatelet therapy duration.
Specific Activation of the Alternative Cardiac Promoter of
Cacna1c
by the Mineralocorticoid Receptor
Authors: Mesquita Thassio R. , Auguste Gaëlle , Falcón Débora , Ruiz-Hurtado Gema , Salazar-Enciso Rogelio , Sabourin Jessica , Lefebvre Florence , Viengchareun Say , Kobeissy Hussein , Lechène Patrick , Nicolas Valérie , Fernandez-Celis Amaya , Gómez Susana , Lauton Santos Sandra , Morel Eric , Rueda Angelica , López-Andrés Natalia , Gómez Ana Maria , Lombès Marc , Benitah Jean-Pierre
Published: February 2018

Rationale:

The MR (mineralocorticoid receptor) antagonists belong to the current therapeutic armamentarium for the management of cardiovascular diseases, but the mechanisms conferring their beneficial effects are poorly understood. Part of the cardiovascular effects of MR is because of the regulation of L-type Ca
v
1.2 Ca
2+
channel expression, which is generated by tissue-specific alternative promoters as a long cardiac or short vascular N-terminal transcripts.

Objective:

To analyze the molecular mechanisms by which aldosterone, through MR, modulates Ca
v
1.2 expression and function in a tissue-specific manner.

Methods and Results:

In primary cultures of neonatal rat ventricular myocytes, aldosterone exposure for 24 hours increased in a concentration-dependent manner long cardiac Ca
v
1.2 N-terminal transcripts expression at both mRNA and protein levels, correlating with enhanced concentration-, time-, and MR-dependent P1-promoter activity. In silico analysis and mutagenesis identified MR interaction with both specific activating and repressing DNA-binding elements on the P1-promoter. The relevance of this regulation is confirmed both ex and in vivo in transgenic mice harboring the luciferase reporter gene under the control of the cardiac P1-promoter. Moreover, we show that this
cis
-regulatory mechanism is not limited to the heart. Indeed, in smooth muscle cells from different vascular beds, in which the short vascular Cav1.2 N-terminal transcripts is normally the major isoform, we found that MR signaling activates long cardiac Ca
v
1.2 N-terminal transcripts expression through P1-promoter activation, leading to vascular contractile dysfunction. These results were further corroborated in hypertensive aldosterone/salt rodent models, showing notably a positive correlation between blood pressure and cardiac P1-promoter activity in aorta. This new vascular long cardiac Ca
v
1.2 N-terminal transcripts molecular signature reduced sensitivity to the Ca
2+
channel blocker, nifedipine, in aldosterone-treated vessels.

Conclusions:

Our results reveal that MR acts as a transcription factor to translate aldosterone signal into specific cardiac P1-promoter activation that might influence the therapeutic outcome of cardiovascular diseases.
The European Union as an Actor in the Law of the Sea, with Particular Reference to the Arctic
Authors: Churchill Robin
Published: February 2018

The first part of this article explores the extent to which the European Union (EU) is an actor in the law of the sea. After explaining when, why and how the EU became such an actor, it considers the legal and political constraints on the capacity of the EU to act; the interests that have shaped its role as an actor; and the various means by which it acts. The second part of the article applies the conclusions from this analysis to outline the role that the EU has so far played in the ongoing development of the legal regime of the marine Arctic and to predict the role that it will continue to play, especially as regards navigation, fisheries, the exploitation of offshore oil and gas, and the protection of the environment.
Still no Evidence for Sustained Effects of Multisensory Integration of Duration
Authors: de la Rosa Maria D. , Bausenhart Karin M.
Published: February 2018

In studies on temporal order perception, immediate as well as sustained effects of multisensory integration have been demonstrated repeatedly. Regarding duration perception, the corresponding literature reports clear immediate effects of multisensory integration, but evidence on sustained effects of multisensory duration integration is scarce. In fact, a single study [Heron, J. et al. (2013). A neural hierarchy for illusions of time: Duration adaptation precedes multisensory integration, J. Vis. 13, 1–12.] investigated adaptation to multisensory conflicting intervals, and found no sustained effects of the audiovisual conflict on perceived duration of subsequently presented unimodal visual intervals. In two experiments, we provide independent evidence in support of this finding. In Experiment 1, we demonstrate that adaptation to audiovisual conflict does not alter perceived duration of subsequently presented visual test intervals. Thus, replicating the results of Heron et al. (2013), we observed no sustained effect of multisensory duration integration. However, one might argue that the prolonged exposure to consistent multisensory conflict might have prevented or hampered multisensory integration per se. In Experiment 2, we rule out this alternative explanation by showing that multisensory integration of audiovisual conflicting intervals is still effective after exposure to audiovisual conflict. This further strengthens the conclusion that multisensory integration of interval duration affects perception in an immediate, but not in a sustained manner.
The genus Alphocoris in the Indomalaya (Hemiptera: Heteroptera: Scutelleridae)
Authors: RÉDEI DÁVID , TSAI JING-FU , JINDRA ZDENĚK
Published: February 2018

The Indomalayan species of the genus Alphocoris Germar, 1839 (Hemiptera: Heteroptera: Scutelleridae: Odontotarsinae: Odontotarsini) are revised. Three species, A. caudatus Rédei, Tsai & Jindra, sp. nov. (India: Maharashtra), A. naso Rédei & Tsai, sp. nov. (India: Goa), and A. asper Rédei, Tsai & Jindra, sp. nov. (India: Goa and Maharashtra), are recognized. The type material of A. lixoides Germar, 1839 (type locality: Senegal) is documented; previous records of this species from India and Pakistan are considered as based on misidentifications, the species is restricted to the Afrotropical Region.
The larvae of three Greek species of Hydropsyche (Trichoptera: Hydropsychidae) and key for larvae of known Aegean Hydropsyche species
Authors: KARAOUZAS IOANNIS
Published: February 2018

The larvae of Hydropsyche cetibeli Malicky & Sipahiler 1993 from the Aegean, Hydropsyche mostarensis Klapálek 1898, endemic of the Balkan Peninsula, and Hydropsyche pygmalion Malicky 2001 endemic of Serifos Island (Cyclades, Greece) are described for the first time. The diagnostic features of the species are described and illustrated, and some information regarding their ecology is included. In addition, a tabular key for larvae of the known Hydropsyche species of Greece and the Aegean Islands is included.
Role of Eastern Ghats Orography and Cold Pool in an Extreme Rainfall Event over Chennai on 1 December 2015
Authors: Phadtare Jayesh
Published: February 2018

Chennai and its surrounding region received extreme rainfall on 1 December 2015. A rain gauge in the city recorded 494 mm of rainfall within a span of 24 h—at least a 100-yr event. The convective system was stationary over the coast during the event. This study analyzes how the Eastern Ghats orography and moist processes localized the rainfall. ERA-Interim data show a low-level easterly jet (LLEJ) over the adjacent ocean and a barrier jet over the coast during the event. A control simulation with the nonhydrostatic Weather Research and Forecasting (WRF) Model shows that the Eastern Ghats obstructed the precipitation-driven cold pool from moving downstream, resulting in the cold pool piling up and remaining stationary in the upwind direction. The cold pool became weak over the ocean. It stratified the subcloud layer and decelerated the flow ahead of the orography; hence, the flow entered a blocked regime. Maximum deceleration of the winds and uplifting happened at the edge of the cold pool over the coast. Therefore, a stationary convective system and maximum rainfall occurred at the coast. As a result of orographic blocking, propagation of a low pressure system (LPS) was obstructed. Because of the topographic β effect, the LPS subsequently traveled a southward path. In a sensitivity experiment without the orography, the cold pool was swept downstream by the winds; clouds moved inland. In the second experiment with no evaporative cooling of rain, the cold pool did not form; flow, as well as clouds, moved over the orography.
No Rest for the Nasty: Mentoring as Mobilizing for Change and Advocacy
Authors: Miville Marie L.
Published: February 2018

This paper presents the continuing need for effective mentoring in the face of social justice inequities in higher education and the larger society. The importance of persistence and resistance as essential qualities of mentors and mentees is emphasized, as well as the integral links of the personal, political, and professional components of psychological work. A discussion of impactful mentors is presented, including family, academic, cultural, professional, and peer mentors. A final section focuses on current mentees, who in turn will mentor future generations.
Media Democratization in Ecuador
Authors: Anderson Tim
Published: February 2018

A regulatory process of “democratizing the media” based on recent constitutional guarantees and a 2013 communications law is under way in Ecuador. The initiative comes from a demand for new forms of social accountability and participation in the mass media after the Latin American experience of media companies’direct engagement in coups and the destabilization of progressive governments. Media democratization is seen as necessary for the construction of democratic societies. It is distinct in Latin America from recent Northern approaches, which tend to be technocratic, suggesting democratic transformation through new online media and enhanced consumer options. Ecuador’s process follows similar initiatives in Venezuela, Bolivia, Argentina, and Uruguay but is perhaps more articulate and systematic. It is instructive in that it builds on well-established public policy themes of the containment of monopoly power, redress of civil wrongs, and the promotion of participation and diversity. While media corporations mostly seek to disqualify debate on media regulation, Ecuador’s approach deserves closer examination.

Ecuador está llevando a cabo un proceso de “democratización de medios” basado en las recientes garantías constitucionales y la ley de comunicaciones de 2013. La iniciativa responde a una exigencia de nuevas formas de responsabilidad social y participación en los medios de comunicación masivos a raíz de experiencias latinoamericanas en las cuales ciertas compañías de medios han intervenido para desestabilizar o generar golpes de estado contra gobiernos progresistas. La democratización de los medios se considera necesaria para la construcción de sociedades democráticas, y esta aproximación se distingue de aquellas características del hemisferio norte con sus tendencias tecnocráticas, que sugieren que la transformación democrática se ha de llevar a cabo mediante nuevos medios en línea y opciones de consumo más amplias. El proceso ecuatoriano se suma a iniciativas similares en Venezuela, Bolivia, Argentina y Uruguay, pero es quizá más articulado y sistemático. Se basa en temas de política pública establecidos como la contención del poder monopólico, la rectificación de delitos civiles, y la promoción de la participación y la diversidad. Si bien las corporaciones de medios han buscado descalificar los debates en torno a la mencionada regulación, los esfuerzos ecuatorianos merecen ser examinados más de cerca.
Analysis of Graft Length Change Patterns in Medial Patellofemoral Ligament Reconstruction via a Fluoroscopic Guidance Method
Authors: Matsushita Takehiko , Araki Daisuke , Hoshino Yuichi , Oka Shinya , Nishida Kyohei , Tanaka Toshikazu , Miyaji Nobuaki , Kanzaki Noriyuki , Ibaraki Kazuyuki , Matsumoto Tomoyuki , Kurosaka Masahiro , Kuroda Ryosuke
Published: February 2018

Background:

A fluoroscopic guidance method for medial patellofemoral ligament (MPFL) reconstruction has been widely used to determine the anatomic femoral attachment site.

Purpose:

To examine the graft length change patterns in MPFL reconstruction with a fluoroscopic guidance method.

Study Design:

Cross-sectional study; Level of evidence, 3.

Methods:

Forty-four knees of 42 patients who underwent MPFL reconstruction for the treatment of recurrent patellar dislocation were examined prospectively. During surgery, suture anchors were inserted into the proximal one-third and center of the patella. A guide pin for the femoral tunnel was inserted into the position reported by Schöttle et al based on the true lateral view of the knee under fluoroscopic control. Changes in graft length patterns of the proximal and center anchors were examined through 0° to 120° of knee flexion. Favorable changes in length patterns were defined as meeting 2 of 3 criteria: (1) not long during flexion (≤3 mm between 30° and 120° of flexion) and either (2) nearly isometric during flexion between 0° and 90° or (3) slightly long during maximum extension (≤3 mm). Other patterns were considered unfavorable. If the change in length pattern was unfavorable, then the pin for the femoral tunnel was moved to different positions until it was favorable. Knees were separated into the favorable group and the unfavorable group. Differences between the groups regarding radiographic parameters were assessed. Student t test or chi-square test was used for statistical analysis.

Results:

Of the 44 knees, 31 (70.5%) showed favorable patterns. However, 13 knees (29.5%) showed unfavorable patterns; therefore, the position of the pin was changed. The mean ± SD distance from the original position to the final position was 5.3 ± 1.1 mm distal for 7 patients and 5.2 ± 0.4 mm posterodistal for 6 patients. Technical errors, including a nontrue lateral view and the tip of the wire not being in the determined area, were found for 4 of 13 knees in the unfavorable group. There was no statistical difference in radiographic parameters between the groups.

Conclusion:

The graft length change pattern could be nonphysiologic at the position determined through the fluoroscopic guidance method; thus, caution may be necessary. The change in length pattern should be checked before graft fixation. If the length change pattern is unfavorable, then it is advisable to move it approximately 5 to 7 mm distally or posterodistally from the first position.
Searching for Evidence of an Anti-Inflammatory Diet in Children: A Systematic Review of Randomized Controlled Trials for Pediatric Obesity Interventions With a Focus on Leptin, Ghrelin, and Adiponectin
Authors: Lewis Kimberly A. , Brown Sharon A.
Published: February 2018

Problem:

To address the complex phenomenon of pediatric obesity, one must understand the physiological mechanisms regulating energy intake and inflammation. The peptide hormones leptin, ghrelin, and adiponectin are involved in both, but their functions are dysregulated in obesity. The purpose of this systematic review is (1) to characterize studies of nutrition interventions for weight management in children who measure these peptides as outcomes, (2) to assess risk of bias in the studies, and (3) to determine the relationships between these peptides and body mass index (BMI).

Eligibility Criteria:

Peer-reviewed articles written in English, published in 2001–2016, and describing randomized controlled trials of pediatric interventions involving a nutrition component with the outcome measures leptin, ghrelin, and/or adiponectin were included. Articles were excluded if the intervention involved pharmaceuticals, supplements, infant formula, breastfeeding, or surgery.

Sample:

The 25 international studies represented 2,153 obese children.

Results:

Ten diets were identified. Successful interventions included both structured exercise and hypocaloric dietary components, with or without counseling, resistance training, or medical components. Direct measures of adiposity were used in 69% of studies. Comparison group designs were disparate. Leptin levels decreased as BMI decreased. Evidence regarding the relationships of ghrelin and adiponectin with BMI was inconclusive.

Conclusions:

Despite known effects of maturation on hormones, studies did not consistently differentiate findings by maturational stage. Common anti-inflammatory and disease risk modification diets were missing or underrepresented. Studies that include children with comorbidities are needed. BMI and leptin levels have a positive relationship, but evidence on ghrelin and adiponectin was inconclusive.
Inherited Cancer in the Age of Next-Generation Sequencing
Authors: Price Kristin S. , Svenson Ashley , King Elisabeth , Ready Kaylene , Lazarin Gabriel A.
Published: February 2018

Next-generation sequencing (NGS) technology has led to the ability to test for multiple cancer susceptibility genes simultaneously without significantly increasing cost or turnaround time. With growing usage of multigene testing for inherited cancer, ongoing education for nurses and other health-care providers about hereditary cancer screening is imperative to ensure appropriate testing candidate identification, test selection, and posttest management. The purpose of this review article is to (1) provide an overview of how NGS works to detect germline mutations, (2) summarize the benefits and limitations of multigene panel testing, (3) describe risk categories of cancer susceptibility genes, and (4) highlight the counseling considerations for patients pursuing multigene testing.
Polymorphisms in Estrogen Synthesis Genes and Symptom Clusters During the Menopausal Transition and Early Postmenopause: Observations From the Seattle Midlife Women’s Health Study
Authors: Woods Nancy Fugate , Cray Lori A. , Mitchell Ellen Sullivan , Farrin Fred , Herting Jerald
Published: February 2018

During the menopausal transition and early postmenopause, participants in the Seattle Midlife Women’s Health Study (SMWHS) experienced one of the three symptom severity clusters identified through latent class analysis: severe hot flashes with moderate sleep, mood, cognitive, and pain symptoms (high-severity hot flash); low-severity hot flashes with moderate levels of all other symptom groups (moderate severity); and low levels of all symptom groups (low severity). In an effort to determine whether gene polymorphisms were associated with these symptom severity classes, we tested associations between gene polymorphisms in the estrogen synthesis pathways (cytochrome P450 19 [CYP 19] and 17 beta hydroxysteroid dehydrogenase [ 17HSDB1]) and the three symptom severity clusters. SMWHS participants ( N = 137) recorded symptoms monthly in diaries and provided buccal smears for genotyping. Multilevel latent class analysis with multinomial regression was used to determine associations between gene polymorphisms and symptom severity clusters. Only the 17HSDB1 polymorphisms ( rs615942 and rs592389) were associated significantly with the high-severity hot flash cluster versus the low-severity symptom cluster. None of the polymorphisms was associated with the moderate-severity cluster versus the low-severity symptom cluster. Findings of associations of the 17HSDB1 polymorphisms with the high-severity hot flash symptom cluster are consistent with those of an association between 17HSDB1 polymorphisms and hot flashes in the Study of Women and Health Across the Nation population and our previous findings of associations between these polymorphisms with greater estrone levels.