bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessment of Energy Credits in LEED-Certified Buildings Based on Certification Levels and Project Ownership
Authors: Gurgun Asli , Arditi David
Published: February 2018

Compared to other categories, the Energy and Atmosphere category contributes the most to the maximum obtainable points in the Leadership in Energy and Environmental Design (LEED) certification system. The objective of the study was to identify the extent to which project teams take advantage of the credits in the Energy and Atmosphere category of LEED. This study analyzes the performance of practitioners in achieving points in the Energy and Atmosphere credits of LEED-New Construction (NC) 2009 for 1500 buildings that received LEED certification in the US. For a better understanding of the credit patterns, the differences in the performance of practitioners are investigated relative to certification levels and project ownership. Achievement in credits is calculated in terms of percent of maximum points (PMP), since the maximum achievable points differ for each credit. Practitioners’ achievements in the credits were ranked as follows: (1) enhanced commissioning, (2) optimized energy performance, (3) enhanced refrigerant management, (4) green power, (5) measurement and verification, and (6) on-site renewable energy. The largest achievement differences were observed in the on-site renewable energy credit. Concerning building ownership, investors were found to optimize mostly energy efficiency and on-site renewable energy, but to mostly skip enhanced refrigerant management. Performance in the measurement and verification credit was similar for all owner types, whereas investors performed differently from corporations, and government agencies in the enhanced commissioning credit. Practitioners who recognize these priorities and differences are expected to be better positioned to make sustainability-related decisions in building design and construction.
Nano-Immobilized Biocatalysts for Biodiesel Production from Renewable and Sustainable Resources
Authors: Kim Keon , Lee Ok , Lee Eun
Published: February 2018

The cost of biodiesel production relies on feedstock cost. Edible oil is unfavorable as a biodiesel feedstock because of its expensive price. Thus, non-edible crop oil, waste oil, and microalgae oil have been considered as alternative resources. Non-edible crop oil and waste cooking oil are more suitable for enzymatic transesterification because they include a large amount of free fatty acids. Recently, enzymes have been integrated with nanomaterials as immobilization carriers. Nanomaterials can increase biocatalytic efficiency. The development of a nano-immobilized enzyme is one of the key factors for cost-effective biodiesel production. This paper presents the technology development of nanomaterials, including nanoparticles (magnetic and non-magnetic), carbon nanotubes, and nanofibers, and their application to the nano-immobilization of biocatalysts. The current status of biodiesel production using a variety of nano-immobilized lipase is also discussed.
Modeling of a Pilot-Scale Fixed-Bed Reactor for Dehydration of 2,3-Butanediol to 1,3-Butadiene and Methyl Ethyl Ketone
Authors: Song Daesung
Published: February 2018

A 1D heterogeneous reactor model accounting for interfacial and intra-particle gradients was developed to simulate the dehydration of 2,3-Butanediol (2,3-BDO) to 1,3-Butadiene (1,3-BD) and Methyl Ethyl Ketone (MEK) over an amorphous calcium phosphate (a-CP) catalyst in a pilot-scale fixed-bed reactor. The developed model was validated with experimental data in terms of a fluid temperature profile along with the length of the catalyst bed, 2,3-BDO conversion, and selectivity for the major products, 1,3-BD and MEK, at the outlet of the reactor. The fluid temperature profile obtained from the model along the length of the catalyst bed coincides satisfactorily with the experimental observations. The difference between the experimental data and the 1D heterogeneous reactor model prediction for 2,3-BDO conversion and selectivity of 1,3-BD and MEK were 0.1%, 9 wt %, and 2 wt %, respectively. In addition, valuable insights related to the feeding system of a commercial-scale plant were made through troubleshooting of the pilot tests. Notably, if the feed including only 2,3-BDO and furnaces that increase the temperature of the feed to the reaction temperature were used in a commercial plant, the feeding system could not be operational because of the presence of heavy chemicals considered oligomers of 2,3-BDO.
Application of Graphene-Oxide-Modified Polyacrylate Polymer for Controlled-Release Coated Urea
Authors: Yuan Wenjun , Shen Yazhen , Ma Fei , Du Changwen
Published: February 2018

Polyacrylate polymer (PA) was modified with graphene oxide (GO) and the obtained composites were applied as coatings for controlled-release coated urea (CRU). The physicochemical properties of the different PA/GO coatings were characterized in detail and the nitrogen-release characteristics of the obtained CRU samples were determined in water at 25 °C. The experimental results revealed that addition of GO to PA reduced the swelling degree from 83.01% to 46.35% and improved its mechanical properties (the Young’s modulus was improved from 31.52 to 34.97 MPa and the glass transition temperature was increased from 4.21 to 6.11 °C), thus dramatically slowing down the cumulative nutrient release from the CRU fertilizer from 87.25% to 59.71%. These results suggest that GO enhances the properties of PA for CRU applications, which shows that GO-modified PA is a good coating material.
Porous Zinc Oxide Thin Films: Synthesis Approaches and Applications
Authors: Laurenti Marco , Cauda Valentina
Published: February 2018

Zinc oxide (ZnO) thin films have been widely investigated due to their multifunctional properties, i.e., catalytic, semiconducting and optical. They have found practical use in a wide number of application fields. However, the presence of a compact micro/nanostructure has often limited the resulting material properties. Moreover, with the advent of low-dimensional ZnO nanostructures featuring unique physical and chemical properties, the interest in studying ZnO thin films diminished more and more. Therefore, the possibility to combine at the same time the advantages of thin-film based synthesis technologies together with a high surface area and a porous structure might represent a powerful solution to prepare ZnO thin films with unprecedented physical and chemical characteristics that may find use in novel application fields. Within this scope, this review offers an overview on the most successful synthesis methods that are able to produce ZnO thin films with both framework and textural porosities. Moreover, we discuss the related applications, mainly focused on photocatalytic degradation of dyes, gas sensor fabrication and photoanodes for dye-sensitized solar cells.
A Localized Meshless Technique for Generating 3-D Wind Fields
Authors: Pepper Darrell , Ramos Gonzalez Maria
Published: February 2018

A localized meshless method is used to simulate 3-D atmospheric wind fields for wind energy assessment and emergency response. The meshless (or mesh-free) method with radial basis functions (RBFs) alleviates the need to create a mesh required by finite difference, finite volume, and finite element methods. The method produces a fast solution that converges with high accuracy, establishing 3-D wind estimates over complex terrain. The method does not require discretization of the domain or boundary and removes the need for domain integration. The meshless method converges exponentially for smooth boundary shapes and boundary data, and is insensitive to dimensional constraints. Coding of the method is very easy and can be done using MATLAB or MAPLE. By employing a localized RBF procedure, 3-D wind fields can be established from sparse meteorological data. The meshless method can be easily run on PCs and hand-held mobile devices. This article summarizes previous work where the meshless method has successfully simulated 3D wind fields over various environments, along with the equations used to obtain the simulations.
Carotid Artery Calcification: A Digital Panoramic-Based Study
Authors: Nasseh Ibrahim , Aoun Georges
Published: February 2018

Objective: The aim of this study was to estimate the incidence of carotid artery calcification (CAC) in a sample of Lebanese population using digital panoramic radiographs. Materials and Methods: Panoramic radiographs of 500 patients (281 females and 219 males), aged between 18 and 88 years (mean: 47.9 years), were assessed for CAC. Data collected were analyzed statistically using IBM® SPSS® for Windows version 20.0 (SPSS, Chicago, IL, USA). Results: CAC were found in 34 cases (6.8%), among them, 23 females (8.18%) and 11 males (5.02%). Six of all the calcifications were on the right side, against six on the left side, and 22 on both sides. The mean age of patients affected with CAC was 60.9 years (ranging from 18 to 88 years). Chi-square test showed no statistical significance between gender and CAC, while Spearman correlation analysis showed positive low correlation with age (r = 0.179). Conclusion: CAC can be found on routine panoramic radiographs taken in dental clinics; dentists should automatically refer the patients in question for specialized medical evaluation.
Neural Network Based Maximum Power Point Tracking Control with Quadratic Boost Converter for PMSG—Wind Energy Conversion System
Authors: Tiwari Ramji , Krishnamurthy Kumar , Neelakandan Ramesh , Padmanaban Sanjeevikumar , Wheeler Patrick
Published: February 2018

This paper proposes an artificial neural network (ANN) based maximum power point tracking (MPPT) control strategy for wind energy conversion system (WECS) implemented with a DC/DC converter. The proposed topology utilizes a radial basis function network (RBFN) based neural network control strategy to extract the maximum available power from the wind velocity. The results are compared with a classical Perturb and Observe (P&O) method and Back propagation network (BPN) method. In order to achieve a high voltage rating, the system is implemented with a quadratic boost converter and the performance of the converter is validated with a boost and single ended primary inductance converter (SEPIC). The performance of the MPPT technique along with a DC/DC converter is demonstrated using MATLAB/Simulink.
Improving Vegetable Oil Properties by Transforming Fatty Acid Chain Length in Jatropha Oil and Coconut Oil Blends
Authors: Wahyudi  , Wardana I.N.G. , Widodo Agung , Wijayanti Widya
Published: February 2018

Efforts to improve the physical and chemical properties of vegetable oils as diesel fuels such as viscosity and calorific value are indispensable with the depletion of fossil oil reserves. Jatropha oil with long chain fatty acids and high degree of unsaturation is mixed with short chain saturated fatty acid coconut oil in various compositions. The mixture was heated and stirred for 30 min at 90 °C. This mixing leads to a decrease in viscosity which allows for the breaking of the bond. The fatty acid molecule structure undergoes transformation that changes the degree of unsaturation and the average length of the carbon chain. Consequently, the kinematic viscosity and flash point of the mixture decreases while its calorific value increases.
Assessment of the Joint Development Potential of Wave and Wind Energy in the South China Sea
Authors: Wan Yong , Fan Chenqing , Dai Yongshou , Li Ligang , Sun Weifeng , Zhou Peng , Qu Xiaojun
Published: February 2018

The South China Sea is a major shipping hub between the West Pacific and Indian Oceans. In this region, the demand for energy is enormous, both for residents’ daily lives and for economic development. Wave energy and wind energy are two major clean and low-cost ocean sources of renewable energy. The reasonable development and utilization of these energy sources can provide a stable energy supply for coastal cities and remote islands of China. Before wave energy and wind energy development, however, we must assess the potential of each of these sources. Based on high-resolution and high-accuracy wave field data and wind field data obtained by ERA-Interim reanalysis for the recent 38-year period from 1979–2016, the joint development potential of wave energy and wind energy was assessed in detail for offshore and nearshore areas in the South China Sea. Based on potential installed capacity, the results revealed three promising areas for the joint development of nearshore wave energy and wind energy, including the Taiwan Strait, Luzon Strait and the sea southeast of the Indo-China Peninsula. For these three dominant areas (key stations), the directionality of wave energy and wind energy propagation were good in various seasons; the dominant wave conditions and the dominant wind conditions were the same, which is advantageous for the joint development of wave and wind energy. Existing well-known wave energy converters (WECs) are not suitable for wave energy development in the areas of interest. Therefore, we must consider the distributions of wave conditions and develop more suitable WECs for these areas. The economic and environmental benefits of the joint development of wave and wind energy are high in these promising areas. The results described in this paper can provide references for the joint development of wave and wind energy in the South China Sea.
A Traveling-Wave-Based Fault Location Scheme for MMC-Based Multi-Terminal DC Grids
Authors: Zhang Shuo , Zou Guibin , Huang Qiang , Gao Houlei
Published: February 2018

This paper presents a novel fault location scheme of DC line in modular multilevel converter (MMC)-based multi-terminal DC (MTDC) grids. Considering the low-inertia characteristics and the meshed topology, the scheme, based on traveling-wave principle, is divided into three steps, namely, faulty pole identification, faulty segment determination and fault-distance calculation. With accurate amplitude, polarities and arrival times of the first arrival current traveling waves (FACTWs) collected from time-synchronized measurements taken just at the converter stations, the proposed scheme can correctly determine the faulty pole, the faulty segment and the precise fault location. The continuous wavelet transform (CWT) is deployed to extract the required features of the input signals at the DC lines. Since the scheme merely needs the features of FACTWs, the practical difficulties of detecting subsequent traveling waves are avoided. A four-terminal MMC-based high voltage direct current (HVDC) grid was built in PSCAD/EMTDC software to evaluate the performance of the fault-location scheme. Simulation results for different cases demonstrate that the proposed fault-location scheme has high accuracy, good adaptability and reliability. Furthermore, the algorithm can be used for a MMC-MTDC grid with any number of meshes.
A Unified Control Strategy for Inductor-Based Active Battery Equalisation Schemes
Authors: Liu Xintian , Wan Zhihao , He Yao , Zheng Xinxin , Zeng Guojian , Zhang Jiangfeng
Published: February 2018

Series battery equalisation can improve battery charge and discharge reliability and extend battery life. Inductor-based battery equalisation schemes have the advantages of simple topologies and control strategies. According to the energy transfer pathway, inductor-based battery equalisation schemes can be divided into cell-to-cell and cell-to-pack equalisation schemes. The control strategies of the cell-to-cell schemes are simple; the inductor can only transfer energy between the neighbouring cells, so the equalisation speed is low. The cell-to-pack schemes are able to accomplish energy transfer between the cells and pack by charging and discharging the inductors. The equalisation speed is high, but the control strategies may be complex. In this paper, different equalisation topologies are reviewed, then a unified control strategy which is applicable to all of the inductor-based equalisation topologies is proposed. The equalisation speeds and efficiencies of these different schemes, including the newly-proposed unified control strategy, are analysed and compared. Based on the theoretical analysis, simulations, and experimental verifications, it is concluded that this unified control strategy can perform the battery equalisation process quickly and efficiently.
Coherent Vortical Structures and Their Relation to Hot/Cold Spots in a Thermal Turbulent Channel Flow
Authors: Dharmarathne Suranga , Pulletikurthi Venkatesh , Castillo Luciano
Published: February 2018

Direct numerical simulations of a turbulent channel flow with a passive scalar at R e τ = 394 with blowing perturbations is carried out. The blowing is imposed through five spanwise jets located near the upstream end of the channel. Behind the blowing jets (about 1 D , where D is the jet diameter), we observe regions of reversed flow responsible for the high temperature region at the wall: hot spots that contribute to further heating of the wall. In between the jets, low pressure regions accelerate the flow, creating long, thin, streaky structures. These structures contribute to the high temperature region near the wall. At the far downstream of the jet (about 3 D ), flow instabilities (high shear) created by the blowing generate coherent vortical structures. These structures move hot fluid near the wall to the outer region of the channel; thereby, these are responsible for cooling of the wall. Thus, for engineering applications where cooling of the wall is necessary, it is critical to promote the generation of coherent structures near the wall.
Elementary Flow Field Profiles of Micro-Swimmers in Weakly Anisotropic Nematic Fluids: Stokeslet, Stresslet, Rotlet and Source Flows
Authors: Kos Žiga , Ravnik Miha
Published: February 2018

Analytic formulations of elementary flow field profiles in weakly anisotropic nematic fluid are determined, which can be attributed to biological or artificial micro-swimmers, including Stokeslet, stresslet, rotlet and source flows. Stokes equation for a nematic stress tensor is written with the Green function and solved in the k-space for anisotropic Leslie viscosity coefficients under the limit of leading isotropic viscosity coefficient. Analytical expressions for the Green function are obtained that are used to compute the flow of monopole or dipole swimmers at various alignments of the swimmers with respect to the homogeneous director field. Flow profile is also solved for the flow sources/sinks and source dipoles showing clear emergence of anisotropy in the magnitude of flow profile as the result of fluid anisotropic viscosity. The range of validity of the presented analytical solutions is explored, as compared to exact numerical solutions of the Stokes equation. This work is a contribution towards understanding elementary flow motifs and profiles in fluid environments that are distinctly affected by anisotropic viscosity, offering analytic insight, which could be of relevance to a range of systems from microswimmers, active matter to microfluidics.
A Qualitative Exploration of the Role of Vape Shop Environments in Supporting Smoking Abstinence
Authors: Ward Emma , Cox Sharon , Dawkins Lynne , Jakes Sarah , Holland Richard , Notley Caitlin
Published: February 2018

E-cigarettes are the most popular method of quitting smoking in England and most are purchased in specialist vape shops. This qualitative study explores how the vape shop environment is experienced by quitters to support smoking abstinence. Semi-structured qualitative interviews were conducted to elicit experiences of e-cigarette use, including experiences of vape shops, in 40 people who had used e-cigarettes in a quit attempt. Observations of six shops in a range of locations were also undertaken. Interview and observation data were analysed using inductive thematic analysis and triangulated. At an individual level, smoking abstinence was supported through shop assistants’ attempts to understand customers’ smoking preferences in order to: (i) tailor advice about the most appropriate product; and (ii) offer an ongoing point of contact for practical help. At an interpersonal level, shops offered opportunity to socialise and reinforce a vaping identity, although the environment was perceived as intimidating for some (e.g., new and female users). At a structural level, shops ensured easy access to products perceived to be good value by customers and had adapted to legislative changes. Vape shops can provide effective behavioural support to quitters to maintain smoking abstinence. Health professionals could capitalise on this through partnership working with shops, to ensure best outcomes for clients wanting to use e-cigarettes to quit smoking.
Taking Action on Air Pollution Control in the Beijing-Tianjin-Hebei (BTH) Region: Progress, Challenges and Opportunities
Authors: Wang Li , Zhang Fengying , Pilot Eva , Yu Jie , Nie Chengjing , Holdaway Jennifer , Yang Linsheng , Li Yonghua , Wang Wuyi , Vardoulakis Sotiris , Krafft Thomas
Published: February 2018

Due to rapid urbanization, industrialization and motorization, a large number of Chinese cities are affected by heavy air pollution. In order to explore progress, remaining challenges, and sustainability of air pollution control in the Beijing-Tianjin-Hebei (BTH) region after 2013, a mixed method analysis was undertaken. The quantitative analysis comprised an overview of air quality management in the BTH region. Semi-structured expert interviews were conducted with 12 stakeholders from various levels of government and research institutions who played substantial roles either in decision-making or in research and advising on air pollution control in the BTH region. The results indicated that with the stringent air pollution control policies, the air quality in BTH meets the targets of the Air Pollution Prevention and Control Action Plan. However, improvements vary across the region and for different pollutants. Although implementation has been decisive and was at least in parts effectively enforced, significant challenges remained with regard to industrial and traffic emission control, and national air quality limits continued to be significantly exceeded and competing development interests remained mainly unsolved. There were also concerns about the sustainability of the current air pollution control measures especially for industries due to the top-down enforcement, and the associated large burden of social cost including unemployment and social inequity resulting industrial restructuring. Better mechanisms for ensuring cross-sectoral coordination and for improved central-local government communication were suggested. Further suggestions were provided to improve the conceptual design and effective implementation of respective air pollution control strategies in BTH. Our study highlights some of the major hurdles that need to be addressed to succeed with a comprehensive air pollution control management for the Chinese mega-urban agglomerations.
Integrated Supervision of the Financial Market without the UK?
Authors: Janovec Michal
Published: February 2018

This paper analyses the integration of financial market supervision at international level, particularly focusing on EU law and the actual processes taking place in this area considering Brexit as its part. Current legislative action at EU level has a significant impact on legislation in all member countries of European Union. This paper seeks, among other things, to find the causes of the increasingly ongoing process of integration of financial market supervision and determine whether or not the direction in which the international integration is going is the right one. The objective of this paper is to determine whether or not the process of integration increases the efficiency of financial market supervision itself and helps to develop the European single market, while simultaneously reducing systemic risk to financial market stability.
Holo3DGIS: Leveraging Microsoft HoloLens in 3D Geographic Information
Authors: Wang Wei , Wu Xingxing , Chen Guanchen , Chen Zeqiang
Published: February 2018

Three-dimensional geographic information systems (3D GIS) attempt to understand and express the real world from the perspective of 3D space. Currently, 3D GIS perspective carriers are mainly 2D and not 3D, which influences how 3D information is expressed and further affects the user cognition and understanding of 3D information. Using mixed reality as a carrier of 3D GIS is promising and may overcome problems when using 2D perspective carriers in 3D GIS. The objective of this paper is to propose an architecture and method to leverage the Microsoft HoloLens in 3D geographic information (Holo3DGIS). The architecture is designed according to three processes for developing holographic 3D GIS; the three processes are the creation of a 3D asset, the development of a Holo3DGIS application, and the compiler deployment of the Holo3DGIS application. Basic geographic data of Philadelphia were used to test the proposed methods and Holo3DGIS. The experimental results showed that the Holo3DGIS can leverage 3D geographic information with the Microsoft HoloLens. By changing the traditional 3D geographic information carrier from a 2D computer screen perspective to mixed reality glasses using the HoloLens 3D holographic perspective, it changed the traditional vision, body sense, and interaction modes, which enables GIS users to experience real 3D GIS.
Inflammatory Alteration of Human T Cells Exposed Continuously to Asbestos
Authors: Kumagai-Takei Naoko , Yamamoto Shoko , Lee Suni , Maeda Megumi , Masuzzaki Hidenori , Sada Nagisa , Yu Min , Yoshitome Kei , Nishimura Yasumitsu , Otsuki Takemi
Published: February 2018

Asbestos is a known carcinogen and exposure can lead to lung cancer and malignant mesothelioma. To examine the effects of asbestos fibers on human immune cells, the human T cell leukemia/lymphoma virus (HTLV)-1 immortalized human T cell line MT-2 was employed. Following continuous exposure to asbestos fibers for more than eight months, MT-2 sublines showed acquisition of resistance to asbestos-induced apoptosis with decreased death signals and increased surviving signals. These sublines showed various characteristics that suggested a reduction in anti-tumor immunity. On the other hand, inflammatory changes such as expression of MMP7, CXCR5, CXCL13 and CD44 was found to be markedly higher in sublines continuously exposed to asbestos compared with original MT-2 cells. All of these molecules contribute to lung inflammation, T and B cell interactions and connections between mesothelial cells and T cells. Thus, further investigation focusing on these molecules may shed light on the role of chronic inflammation caused by asbestos exposure and the occurrence of malignant mesothelioma. Finally, regarding peripheral T cells from healthy donors (HD) and asbestos-exposed patients with pleural plaque (PP) or malignant pleural mesothelioma (MPM), following stimulation of CD4+ T cells, T cells from MPM patients showed reduced potential of interferon (IFN)-γ expression. Moreover, levels of interleukin (IL)-6, one of the most important cytokines in chronic inflammation, in cultured supernatants were higher in PP and MPM patients compared with HD. Overall, asbestos-induced chronic inflammation in the lung as well as the pleural cavity may facilitate the onset of asbestos-induced cancers due to alterations in the interactions among fibers, immune cells such as T and B cells and macrophages, and mesothelial and lung epithelial cells. Further investigations regarding chronic inflammation caused by asbestos fibers may assist in identifying molecular targets for preventive and therapeutic strategies related to the effects of asbestos exposure.
Roles of Two Sox9 Genes during Gonadal Development in Japanese Flounder: Sex Differentiation, Spermatogenesis and Gonadal Function Maintenance
Authors: Li Xiaojing , Yu Haiyang , Wang Yujue , Liu Xiaobing , Liu Yuezhong , Qu Jiangbo , Wang Xubo
Published: February 2018

The transcription factor sox9 has been implicated in cartilage formation and testis determination in mammals. Here, two duplicates of sox9 were found in Japanese flounder (Paralichthys olivaceus) named Posox9a and Posox9b, respectively. Phylogenetic and gene structure analyses revealed that Posox9a and Posox9b were homologous to that of teleosts and tetrapods. Quantitative real-time polymerase chain reaction (qRT-PCR) showed that both Posox9a and Posox9b expressed higher in testis than in ovary of adult tissues. The in situ hybridization (ISH) analysis of gonads showed that Posox9a and Posox9b mRNA were both detected in oocytes, Sertoli cells and spermatocytes. During sex differentiation, the expression of Posox9a exhibited obvious sexual dimorphic expression from 60 days after hatch (dah) with higher expression in male preferred individuals than female preferred individuals and increased gradually from 30 to 100 dah. A similar pattern was detected in Posox9b expression. After injection of androgen (17α-methyltestosterone) of different concentrations, the expression level of Posox9b increased significantly, whereas Posox9a did not change obviously. These results indicated that the two sox9 genes of Japanese flounder had converse functions in sex differentiation, whereas their differences in 17α-methyltestosterone administration were obvious and worthwhile for exploring evolutionary and adaptive significance. This study provided a foundation for further exploration of the roles of Posox9 genes during the sex determination and differentiation, spermatogenesis and gonadal function maintenance of Japanese flounder.
Inhibition of Phosphodiesterase 4 by FCPR03 Alleviates Lipopolysaccharide-Induced Depressive-Like Behaviors in Mice: Involvement of p38 and JNK Signaling Pathways
Authors: Yu Hui , Zou Zhengqiang , Zhang Xiaolin , Peng Wanli , Chen Chen , Ye Yicheng , Xu Jiangping , Wang Haitao
Published: February 2018

Inflammatory responses induced by peripheral administration of lipopolysaccharide (LPS) triggers depressive-like behavioral syndrome in rodents. Inhibition of phosphodiesterase 4 (PDE4) produces a robust anti-inflammatory effect in inflammatory cells. Unfortunately, archetypal PDE4 inhibitors cause intolerable gastrointestinal side-effects, such as vomiting and nausea. N-isopropyl-3-(cyclopropylmethoxy)-4-difluoromethoxy benzamide (FCPR03) is a novel, selective PDE4 inhibitor with little, or no, emetic potency. Our previous studies show that FCPR03 is effective in attenuating neuroinflammation in mice treated with LPS. However, whether FCPR03 could exert antidepressant-like effect induced by LPS is largely unknown. In the present study, mice injected intraperitoneally (i.p.) with LPS was established as an in vivo animal model of depression. The antidepressant-like activities of FCPR03 were evaluated using a tail suspension test, forced swimming test, and sucrose preference test. We demonstrated that administration of FCPR03 (1 mg/kg) produced antidepressant-like effects in mice challenged by LPS, as evidenced by decreases in the duration of immobility in the forced swim and tail suspension tests, while no significant changes in locomotor activity were observed. FCPR03 also increased sucrose preference in mice treated with LPS. In addition, treatment with FCPR03 abolished the downregulation of brain-derived neurotrophic factor induced by LPS and decreased the level of corticosterone in plasma. Meanwhile, periphery immune challenge by LPS induced enhanced phosphorylation of p38-mitogen activated protein kinase (p38) and c-Jun N-terminal kinase (JNK) in both the cerebral cortex and hippocampus in mice. Interestingly, treatment with FCPR03 significantly blocked the role of LPS and reduced the levels of phosphorylated p38 and JNK. Collectively, these results indicate that FCPR03 shows antidepressant-like effects in mice challenged by LPS, and the p38/JNK signaling pathway is possibly involved in this process. Our findings suggest that FCPR03 is a potential compound for the prevention or treatment of depression.
Neurotropin® Accelerates the Differentiation of Schwann Cells and Remyelination in a Rat Lysophosphatidylcholine-Induced Demyelination Model
Authors: Matsuoka Hozo , Tanaka Hiroyuki , Sayanagi Junichi , Iwahashi Toru , Suzuki Koji , Nishimoto Shunsuke , Okada Kiyoshi , Murase Tsuyoshi , Yoshikawa Hideki
Published: February 2018

Neurotropin® (NTP), a non-protein extract of inflamed rabbit skin inoculated with vaccinia virus, is clinically used for the treatment of neuropathic pain in Japan and China, although its effect on peripheral nerve regeneration remains to be elucidated. The purpose of this study was to investigate the effects of NTP on Schwann cells (SCs) in vitro and in vivo, which play an important role in peripheral nerve regeneration. In SCs, NTP upregulated protein kinase B (AKT) activity and Krox20 and downregulated extracellular signal-regulated kinase1/2 activity under both growth and differentiation conditions, enhanced the expression of myelin basic protein and protein zero under the differentiation condition. In a co-culture of dorsal root ganglion neurons and SCs, NTP accelerated myelination of SCs. To further investigate the influence of NTP on SCs in vivo, lysophosphatidylcholine was injected into the rat sciatic nerve, leading to the focal demyelination. After demyelination, NTP was administered systemically with an osmotic pump for one week. NTP improved the ratio of myelinated axons and motor, sensory, and electrophysiological function. These findings reveal novel effects of NTP on SCs differentiation in vitro and in vivo, and indicate NTP as a promising treatment option for peripheral nerve injuries and demyelinating diseases.
The Complete Chloroplast Genome of Catha edulis: A Comparative Analysis of Genome Features with Related Species
Authors: Gu Cuihua , Tembrock Luke , Zheng Shaoyu , Wu Zhiqiang
Published: February 2018

Qat (Catha edulis, Celastraceae) is a woody evergreen species with great economic and cultural importance. It is cultivated for its stimulant alkaloids cathine and cathinone in East Africa and southwest Arabia. However, genome information, especially DNA sequence resources, for C. edulis are limited, hindering studies regarding interspecific and intraspecific relationships. Herein, the complete chloroplast (cp) genome of Catha edulis is reported. This genome is 157,960 bp in length with 37% GC content and is structurally arranged into two 26,577 bp inverted repeats and two single-copy areas. The size of the small single-copy and the large single-copy regions were 18,491 bp and 86,315 bp, respectively. The C. edulis cp genome consists of 129 coding genes including 37 transfer RNA (tRNA) genes, 8 ribosomal RNA (rRNA) genes, and 84 protein coding genes. For those genes, 112 are single copy genes and 17 genes are duplicated in two inverted regions with seven tRNAs, four rRNAs, and six protein coding genes. The phylogenetic relationships resolved from the cp genome of qat and 32 other species confirms the monophyly of Celastraceae. The cp genomes of C. edulis, Euonymus japonicus and seven Celastraceae species lack the rps16 intron, which indicates an intron loss took place among an ancestor of this family. The cp genome of C. edulis provides a highly valuable genetic resource for further phylogenomic research, barcoding and cp transformation in Celastraceae.
Bioreducible Polymer Micelles Based on Acid-Degradable Poly(ethylene glycol)-poly(amino ketal) Enhance the Stromal Cell-Derived Factor-1α Gene Transfection Efficacy and Therapeutic Angiogenesis of Human Adipose-Derived Stem Cells
Authors: Lee Tae-Jin , Shim Min , Yu Taekyung , Choi Kyunghee , Kim Dong-Ik , Lee Soo-Hong , Bhang Suk
Published: February 2018

Adipose-derived stem cells (ADSCs) have the potential to treat ischemic diseases. In general, ADSCs facilitate angiogenesis by secreting various pro-angiogenic growth factors. However, transplanted ADSCs have a low therapeutic efficacy in ischemic tissues due to their poor engraftment and low viability. Stromal cell-derived factor-1α (SDF-1α) improves the survival rate of stem cells transplanted into ischemic regions. In this study, we developed acid-degradable poly(ethylene glycol)-poly(amino ketal) (PEG-PAK)-based micelles for efficient intracellular delivery of SDF-1α plasmid DNA. The SDF-1α gene was successfully delivered into human ADSCs (hADSCs) using PEG-PAK micelles. Transfection of SDF-1α increased SDF-1α, vascular endothelial growth factor, and basic fibroblast growth factor gene expression and decreased apoptotic activity in hADSCs cultured under hypoxic conditions in comparison with conventional gene transfection using polyethylenimine. SDF-1α-transfected hADSCs also showed significantly increased SDF-1α and VEGF expression together with reduced apoptotic activity at 4 weeks after transplantation into mouse ischemic hindlimbs. Consequently, these cells improved angiogenesis in ischemic hindlimb regions. These PEG-PAK micelles may lead to the development of a novel therapeutic modality for ischemic diseases based on an acid-degradable polymer specialized for gene delivery.
Local Patch Vectors Encoded by Fisher Vectors for Image Classification
Authors: Chen Shuangshuang , Liu Huiyi , Zeng Xiaoqin , Qian Subin , Wei Wei , Wu Guomin , Duan Baobin
Published: February 2018

The objective of this work is image classification, whose purpose is to group images into corresponding semantic categories. Four contributions are made as follows: (i) For computational simplicity and efficiency, we directly adopt raw image patch vectors as local descriptors encoded by Fisher vector (FV) subsequently; (ii) For obtaining representative local features within the FV encoding framework, we compare and analyze three typical sampling strategies: random sampling, saliency-based sampling and dense sampling; (iii) In order to embed both global and local spatial information into local features, we construct an improved spatial geometry structure which shows good performance; (iv) For reducing the storage and CPU costs of high dimensional vectors, we adopt a new feature selection method based on supervised mutual information (MI), which chooses features by an importance sorting algorithm. We report experimental results on dataset STL-10. It shows very promising performance with this simple and efficient framework compared to conventional methods.
Preliminary Investigation of Species Diversity of Rice Hopper Parasitoids in Southeast Asia
Authors: Sann Christina , Wemheuer Franziska , Beaurepaire Alexis , Daniel Rolf , Erler Silvio , Vidal Stefan
Published: February 2018

Ongoing intensification of rice production systems in Southeast Asia is causing devastating yield losses each year due to rice hoppers. Their continuing development of immunity to resistant rice varieties and pesticide applications further complicates this problem. Hence, there is a high demand for biological control agents of rice hoppers. Egg parasitoid wasps are among the most important natural enemies of rice hoppers, such as Nilaparvata lugens and Nephotettix spp. However, our knowledge of their diversity is still very limited, due to their small size and the lack of available morphological information. Classifying these parasitoids is the first step to properly understanding their role in the rice agroecosystem. We used traditional morphological identification, as well as DNA sequencing of the 28S rRNA and the COI genes, to investigate the diversity of four important hopper egg parasitoid genera in the Philippines. Parasitoids of the genera Anagrus, Oligosita, Gonatocerus, and Paracentrobia were collected in eight study landscapes located in Luzon. Our findings illustrate that characterization of species diversity using morphological and molecular analyses were concordant only for the genus Paracentrobia. The genera Anagrus and Gonatocerus exhibited more genetic diversity than estimated with the morphological analysis, while the opposite was observed for Oligosita. This is the first study investigating the molecular diversity of rice hopper parasitoids in the Philippines. More research combining morphological, behavioral, and molecular methods, as well as the establishment of a comprehensive DNA database, are urgently needed to assess the performance and suitability of these organisms as biocontrol agents.
Enhanced Sparse Representation-Based Device-Free Localization with Radio Tomography Networks
Authors: Liu Tong , Luo Xiaomu , Liang Zhuoqian
Published: February 2018

The sparse distribution of targets in monitored areas is an important prior for device-free localization (DFL) with radio tomography networks. In this article, our goal is to develop an enhanced sparse representation-based DFL method that takes the full potential of sparsity for location reconstruction. An expanded sensing matrix spanning the concatenation of a sampling matrix and a unit error-correcting base is proposed for modelling the measurement process. The sampling matrix can either be composed of the ellipse model from calibrated networks or the received signal strength (RSS) fingerprint-based model induced by training samples with one person at predefined locations. Thus, the sparsity of targets is enhanced under the expanded sensing matrix and the ℓ 1 -minimization-based approximations are derived for the recovery of locations. Experimental studies in an open outdoor scenario, in a line-of-sight (LOS) indoor scenario, and in a non-line-of-sight (NLOS) indoor scenario, are conducted to verify the efficacy of the proposed method.
On the Cutting Performance of Segmented Diamond Blades when Dry-Cutting Concrete
Authors: Sánchez Egea A. , Martynenko V. , Martínez Krahmer D. , López de Lacalle L. , Benítez A. , Genovese G.
Published: February 2018

The objective of the present study is to analyze and compare the cutting performance of segmented diamond blades when dry-cutting concrete. A cutting criteria is proposed to characterize the wear of the blades by measuring the variation of the external diameter and the weight loss of the blade. The results exhibit the cutting blade SB-A, which has twice the density of diamonds and large contact area, exhibits less wear even though the material removal rate is higher compared with the other two cutting blades. Additionally, the surface topography of the different blades is evaluated to examine the impact of wear depending on the surface profile and the distribution of the diamonds in the blade’s matrix. Large number of diamonds pull-out are found in blades type SB-C, which additionally shows the worst wear resistant capability. As a conclusion, the cutting efficiency of the blade is found to be related to the density of embedded diamonds and the type of the surface profile of the cutting blade after reaching the stop criteria.
Impact of Electrode Surface Morphology in ZnO-Based Resistive Random Access Memory Fabricated Using the Cu Chemical Displacement Technique
Authors: Wu Chi-Chang , You Hsin-Chiang , Lin Yu-Hsien , Yang Chia-Jung , Hsiao Yu-Ping , Liao Tun-Po , Yang Wen-Luh
Published: February 2018

Electrochemical-metallization-type resistive random access memories (ReRAMs) show promising performance as next-generation nonvolatile memory. In this paper, the Cu chemical displacement technique (CDT) is used to form the bottom electrode of ReRAM devices. Compared with conventional deposition methods, the Cu-CDT method has numerous advantages for ReRAM fabrication, including low cost, low temperature fabrication, and the provision of unconsolidated Cu film and large surface roughness. Moreover, the Cu-CDT method is a favorable candidate for overcoming the Cu etching problem and is thus suitable for fabricating ReRAM devices. Using this technique, the surface morphology of a thin Cu film can be easily controlled. The obtained results show that the electric fields during the Forming and SET operations decreased, and the on-state current increased in the RESET operation, as the Cu-CDT displacement time was increased. The Cu-CDT samples exhibited a low operation field, large memory window (>106), and excellent endurance switching cycle characteristics. Moreover, this paper proposes a model to explain the electrical characteristics of ReRAM, which are dependent on the surface morphology.
The Influence of the Heat-Affected Zone Mechanical Properties on the Behaviour of the Welding in Transverse Plate-to-Tube Joints
Authors: Lozano Miguel , Serrano Miguel , López-Colina Carlos , Gayarre Fernando , Suárez Jesús
Published: February 2018

Eurocode 3 establishes the component method to analytically characterize the structural joints between beam and columns. When one of the members involved in the joint is a hollow section (i.e., a tube) there is a lack of information for the specific components present in the joint. There are two different ways to bridge the gap: experimental testing on the actual beam column joints involving tubular sections; or numerical modelization, typically by means of finite element analysis. For this second option, it is necessary to know the actual mechanical properties of the material. As long as the joint implies a welding process, there is a concern related to how the mechanical properties in the heat-affected zone (HAZ) influence the behavior of the joint. In this work, some coupons were extracted from the HAZ of the beam-column joint. The coupons were tested and the results were implemented in the numerical model of the joint, in an attempt to bring it closer to the experimental results of the tested joints.
Alpha-Difluoromethylornithine, an Irreversible Inhibitor of Polyamine Biosynthesis, as a Therapeutic Strategy against Hyperproliferative and Infectious Diseases
Authors: LoGiudice Nicole , Le Linh , Abuan Irene , Leizorek Yvette , Roberts Sigrid
Published: February 2018

The fluorinated ornithine analog α-difluoromethylornithine (DFMO, eflornithine, ornidyl) is an irreversible suicide inhibitor of ornithine decarboxylase (ODC), the first and rate-limiting enzyme of polyamine biosynthesis. The ubiquitous and essential polyamines have many functions, but are primarily important for rapidly proliferating cells. Thus, ODC is potentially a drug target for any disease state where rapid growth is a key process leading to pathology. The compound was originally discovered as an anticancer drug, but its effectiveness was disappointing. However, DFMO was successfully developed to treat African sleeping sickness and is currently one of few clinically used drugs to combat this neglected tropical disease. The other Food and Drug Administration (FDA) approved application for DFMO is as an active ingredient in the hair removal cream Vaniqa. In recent years, renewed interest in DFMO for hyperproliferative diseases has led to increased research and promising preclinical and clinical trials. This review explores the use of DFMO for the treatment of African sleeping sickness and hirsutism, as well as its potential as a chemopreventive and chemotherapeutic agent against colorectal cancer and neuroblastoma.
Alloy Steels
Authors: Tuttle Robert
Published: February 2018

Since their invention in 1865, alloy steels have found broad application in multiple industries; the automotive, aerospace, heavy equipment, and pipeline industries to name a few[...]