bluuebunny/crossref_metadata_2025 huggingface dataset:
Testimonio, experiencia política y feminismo: Ana María Giacosa
Authors: Grasselli Fabiana Hebe , Cabrera Mario Federico David
Published: December 2019

En este artículo nos proponemos analizar la narrativa testimonial de Ana María Giacosa (Jujuy 1940- 1990) atendiendo especialmente a las relaciones entre escritura, género y experiencia política. Con este objetivo, focalizamos nuestro trabajo en un conjunto de relatos publicados a principios de los 80 en el libro Viaje alrededor de mi misma (1982) en los que la autora no solo se propone dar cuenta de su experiencia como militante peronista y presa política sino que además hace explícita la dimensión ética de la escritura en tanto espacio de disputa de memorias colectivas. En este sentido, sostenemos que sus textos desafían y desestabilizan con su existencia el mandato de silencio de la estructura represiva al tiempo que articulan un gesto antipatriarcal tematizando la dimensión sexuada de la experiencia narrada.
Sin palabras: génesis y desarrollos de los estudios sobre la comunicación no verbal
Authors: Pereiro Julio César
Published: December 2019

Desde sus inicios hace casi setenta años los estudios de comunicación no verbal (CNV) se han desarrollado principalmente a partir de los aportes de los campos de la psicología y la antropología. En este sentido, la CNV se ha abocado a la descripción de  aquellos acontecimientos de la comunicación humana que trascienden las palabras dichas o escritas. El presente artículo se propone realizar un recorrido por los principales aportes en materia de CNV, desde el desarrollo inicial de la kinésica, la proxémica y la paralingüística hasta las investigaciones en micromovimientos. Asimismo, se plantea la necesidad de estudiar la CNV desde la perspectiva de la comunicación social, disciplina que ha estado en gran medida ausente en los estudios de la materia.
El clamor de no agresión en Joan Baez y Mercedes Sosa para los oídos argentinos de 1974
Authors: Orjuela David
Published: December 2019

Este trabajo caracterizará el proceso micropolítico sucedido en el recital que la cantante estadounidense Joan Baez dio en Buenos Aires en 1974, el cual contó con la participación de la cantante argentina Mercedes Sosa. El evento sucedió en medio de una crisis política inédita, alentada por luchas ideológicas polarizadas que incubó un estado de violencia permanente. No obstante, ese recital venía como una actualización de, primero que nada, un estado de solidaridad, donde la denuncia social emergía como resultado de una lucha no violenta, de índole más experiencial que formal. Tal posición provocó desencuentros entre la producción artística y una recepción dóxica, dada la distancia percibida entre lo propuesto en el discurso musical de las artistas, y el marco sociopolítico del país. En el recital, el público devenía expectante de determinados efectos de sentido, en parte debido a la estandarizada relación entre contenidos costumbristas y reivindicaciones sociales, a su vez relacionadas con ideologías políticas específicas en el país y en la región. En definitiva, se tomará ese recital como estudio de caso para dar cuenta de cómo una escucha viciada puede acallar la vitalidad del arte, no solo para caracterizar una sociedad, sino para ir más allá y proponer mutaciones políticas oportunas.
Composición y dinámica del empleo en Santiago del Estero 1994-2007
Authors: Gurmendi María Noelia , Salvatierra Rita Gabriela
Published: December 2019

La provincia de Santiago del Estero constituye dentro del país, una región con un peso marginal en la economía nacional (0,8% del PBI del país). Se caracteriza por un secular rezago productivo, con una estructura productiva con escasa participación industrial, lo que se traduce en un débil mercado de trabajo y en un deterioro social que afecta a un porcentaje importante de la población.

En esta investigación se analiza el mercado de trabajo de los sectores productores de bienes y de servicios, los cuales exhiben particularidades y relaciones que ameritan una explicación científica. Nuestro propósito reposa en develar los vínculos que estos mismos establecen, utilizando el PBG (Producto Bruto Geográfico) para Santiago del Estero, como indicador macroeconómico principal, explicando la lógica y dinámica con que se movilizan los recursos entre los sectores a la luz de la teoría.
La construcción estratégica del discurso en comunicación
Authors: Vazquez Bonne Yamila , Saladrigas Medina Hilda Maria
Published: December 2019

El proceso de comunicación se ha descrito con atención a sus componentes: actores, expresiones, representaciones e instrumentos. Esta perspectiva teórica supone una visión estratégica del proceso de la comunicación social, en tanto los discursos resultantes deben ser observados en la interrelación de los indicadores textuales y de los aspectos relativos al co(n) texto del discurso. A la par, sería oportuno asumirlo en la instancia de emisión, en la que se aprecie lo estratégico desde la propia construcción discursiva: construcción estratégica del discurso. Mediante la metodología de análisis estratégico de la construcción del discurso, el artículo analiza un discurso de la Oficina Nacional Tributaria (ONAT) acerca del pago de impuestos en Cuba. Este discurso fundamenta sus estrategias discursivas en la finalidad de convertir a los contribuyentes en parte activa del desarrollo social cubano; así como afianzar su imagen pública como entidad para el bien social.
La construcción de discurso identitario nacional argentino: la historia de la carne
Authors: Forte Diego Luis
Published: December 2019

Las representaciones sociales que construimos y reproducimos a través de los discursos construyen marcos e historias que pautan y limitan nuestras posibilidades de acción en la vida diaria. La Historia de la Carne (Adams, 1990) es el marco que ha regido la perspectiva carnista en la Argentina desde su fundación como nación y es parte de la identidad colectiva del país. Este marco se ha construido durante doscientos años a través de todos los discursos circulantes en el país.

El presente trabajo adopta como marco teórico el Análisis Crítico del Discurso desde la perspectiva propuesta por la Ecolingüística (Stibbe, 2015). Utilizaremos las herramientas de análisis presentadas por Lakoff (2004, 2010) y Kress y van Leeuwen (2006). Nos proponemos analizar la construcción del marco carnista identitario a través de discursos verbales y multimodales de diferentes épocas y presentar discursos alternos.
Memória de um tempo presente sobre o amor animal
Authors: Lopes Stephan Cassiana
Published: December 2019

No presente ensaio, tentaremos entender em que medida a tradição filosófica galgada no princípio de igualdade, atrelado à noção de natureza humana, teria paulatinamente conduzido à justificação e, até mesmo, à fundamentação da desconsideração ético-política do animal e da animalidade. Mais precisamente, a partir de uma perspectiva que interpreta os estudos de Michel Foucault acerca do cuidado de si no período socrático-platônico, sob a luz [A] das análises de Jean-Pierre Vernant no que concerne às diferenças entre a Antiguidade arcaica e a clássica e [B] das reflexões de Judith Butler a propósito da violência moral, indicaremos de que modo a emergência filosófica do princípio de igualdade teria influenciado na estruturação de uma dinâmica moralista que violenta explícita ou implicitamente o animal e, com ele, todos e todas que se distinguem biológica ou eticamente das normas que descrevem o ser humano e que prescrevem a sua humanidade. O nosso artigo é constituído por quatro partes que marcam a temporalidade da questão animal e que manifestam sua urgência no presente histórico. Através da narração de possíveis articulações entre o presente e o passado, buscamos diagnosticar os limites antropocêntricos da filosofia ético-política e, ao mesmo tempo, vislumbrar relações não-antropocêntricas entre humanos e animais.
Tendencia al reemplazo de animales de experimentación
Authors: Rivero Martín Nicolás , Lenze Mariela , Izaguirre Mercedes , Wikinski Silvia Inés , Gutiérrez Maria Laura
Published: December 2019

Para la aprobación, registro y comercialización de medicamentos, agroquímicos, productos de uso doméstico y sustancias cosméticas es necesario estudiar e informar al organismo regulador la potencial toxicidad. Por motivos éticos y económicos, en los últimos años se ha disminuido la utilización de animales de experimentación y se ha fomentado el desarrollo de metodologías alternativas. En el proceso de migración, la OCDE validó procedimientos para evaluar irritación y corrosión ocular y dérmica, mutagenicidad, fototoxicidad, sensibilización y absorción dérmica. Hoy en día es un área en constante investigación y desarrollo ya que aún no se ha logrado reemplazar completamente la calidad de los datos obtenidos en animales. Si bien la tendencia mundial al reemplazo de animales es clara, en Argentina aún no hemos dado el primer paso: no contamos con una ley que regule el uso de animales de experimentación. Las agencias reguladoras (SENASA y ANMAT) continúan solicitando ensayos con animales, pero han manifestado su interés en aceptar evidencia toxicológica obtenida por métodos alternativos. Desde el Laboratorio de Métodos Alternativos al uso de Animales de Experimentación que forma parte de la Plataforma Tecnológica EBAL nos hemos propuesto trabajar en el desarrollo, ejecución, perfeccionamiento y transferencia de metodología alternativa.
Los estudios animales en la literatura hispanoamericana contemporánea
Authors: Lámbarry Alejandro
Published: December 2019

Este artículo tiene como objetivo presentar un esquema general de la aportación de la literatura hispanoamericana contemporánea en la discusión crítica de los estudios animales. Hemos decidido, por motivos metodológicos dividir las obras en tres grupos: la figura del animal 1) cuestiona la ideología antropocentrista, 2) critica su propio estatuto de subordinación, 3) es símbolo de trascendencia mística. Los textos que presentamos –y que he abordado en el transcurso de mis investigaciones sobre el tema del animal en la literatura– son de autores mexicanos y argentinos de finales del siglo XX y comienzos del XXI. Más allá de su particularidad regional, hemos seleccionado a estos autores y a sus obras por su aportación temática a la crítica de los estudios animales.
Milk and Dairy Products Production in Benin
Authors: LANDRY ANİHOUVİ Eudes , SALİH Hanaa , ANİHOUVİ Victor B. , KESENKAŞ Harun
Published: December 2019

The recovery strategy for agricultural sector, which gives priority to the value chain approach, renewed interest in both milk production and processing within the framework of livestock development policy. The consumption of locally produced cow’s milk has grown considerably in recent decades, despite the fact that Benin isn’t a major producer of cattle. The role of milk and milk products in the diet and economy of pastoral communities is well established. However, milk production is still weak and depends on genetic and many other factors. That’s is the reason why, despite its economic and nutritional importance, the Beninese dairy industry is still artisanal. Dairy industry in Benin ensures the marketing of derived products like a local cheese “Wagashi” and a local beverage “Degue”: two derivative products helping to meet the nutritional needs of the population. The production conditions as well as the lack of standardization of the processing and preservation practices are, among other things, critical points justifying the risks associated with the consumption of derived dairy products in Benin. The decline in quantity of domestic imports of milk and milk products in recent decades has led to a segmentation of the dairy markets where local products play an important role. Hence, the need to evaluate the potential of milk production as well as the risks related to the consumption of this with a view to an industrial valorisation involving consumer health safety. The importance of milk and dairy products in the diet, the cattle breeds involved in milk production and their performance and the risks linked to the consumption of dairy products produced in Benin are discussed in this review.
Is the mid-diastolic isthmus always the best ablation target for re-entrant atrial tachycardias?
Authors: De Simone Antonio , Anselmino Matteo , Scaglione Marco , Stabile Giuseppe , Solimene Francesco , De Bellis Antonio , Pepe Marco , Panella Alfonso , Ferraris Federico , Malacrida Maurizio , Maddaluno Francesco , Gaita Fiorenzo , García-Bolao Ignacio
Published: December 2019

Aims

We evaluated the ability of an ultrahigh mapping system to identify the most convenient Rhythmia ablation target (RAT) in intra-atrial re-entrant tachycardias (IART) in terms of the narrowest area to transect to interrupt the re-entry.

Methods

A total of 24 consecutive patients were enrolled with a total of 26 IARTs. The Rhythmia mapping system was used to identify the RAT in all IARTs.

Results

In 18 cases the RAT matched the mid-diastolic phase of the re-entry whereas in 8 cases the RAT differed. In these patients, the mid-diastolic tissue in the active circuit never represented the area with the slowest conduction velocity of the re-entry. The mean conduction velocity at the mid-diastolic site was significantly slower in the group of patients in which the RAT matched the mid-diastolic site (P = 0.0173) and that of the remaining circuit was significantly slower in the group in which the RAT did not match (P = 0.0068). The mean conduction velocity at the RAT was comparable between the two groups (P = 0.66).

Conclusion

Identifying the RAT in challenging IARTs by means of high-density representation of the wavefront propagation of the tachycardia seems feasible and effective. In one-third of cases this approach identifies an area that differs from the mid-diastolic corridor.
Analgesic efficacy of an intravenous constant rate infusion of a morphine-lidocaine-ketamine combination in Holstein calves undergoing umbilical herniorrhaphy
Authors: Hartnack Amanda K. , Niehaus Andrew J. , Lakritz Jeffrey , Coetzee Johann F. , Kleinhenz Michael D.
Published: December 2019

OBJECTIVE

To assess the analgesic efficacy of an IV constant rate infusion (CRI) of a morphine-lidocaine-ketamine (MLK) combination in calves undergoing umbilical herniorrhaphy.

ANIMALS

20 weaned Holstein calves with umbilical hernias.

PROCEDURES

Calves were randomly assigned to receive a CRI of an MLK solution (0.11 mL/kg/h; morphine, 4.8 μg/kg/h; lidocaine, 2.1 mg/kg/h; and ketamine, 0.42 mg/kg/h) for 24 hours (MLK group) or 2 doses of flunixin meglumine (1.1 mg/kg, IV, q 24 h) and a CRI of saline (0.9% NaCl) solution (0.11 mL/kg/h) for 24 hours (control group). The assigned CRI was begun after anesthesia induction. A pain-scoring system and incisional algometry were used to assess pain, and blood samples were obtained to measure serum cortisol concentration at predetermined times for 120 hours after CRI initiation.

RESULTS

Mean pain scores did not differ significantly between the MLK and control groups at any time. Mean algometry score for the MLK group was significantly greater (calves were less responsive to pressure) than that for the control group at 4 hours after CRI initiation. Mean cortisol concentration decreased over time for both groups and was significantly greater for the MLK group than the control group at 1, 4, and 18 hours after CRI initiation.

CONCLUSIONS AND CLINICAL RELEVANCE

A CRI of MLK provided adequate postoperative analgesia to calves that underwent umbilical herniorrhaphy. However, the technical support required for CRI administration limits its use to hospital settings. Kinetic analyses of MLK infusions in cattle are necessary to establish optimal dosing protocols and withdrawal intervals.
Comparison of left and right atrial volumes determined by two- and three-dimensional echocardiography with those determined by multidetector computed tomography for healthy dogs
Authors: Owens Eric J. , LeBlanc Nicole L. , Scollan Katherine F.
Published: December 2019

OBJECTIVE

To compare left atrial volume (LAV) and right atrial volume (RAV) determined by 2-D and 3-D echocardiographic methods with the LAV and RAV determined by ECG-gated multidetector CT (MDCT) for healthy dogs.

ANIMALS

11 healthy purpose-bred young adult hound-type dogs.

PROCEDURES

Each dog was anesthetized and underwent MDCT and a complete echocardiographic examination. Modality-specific software was used to measure the respective atrial volumes at ventricular end systole, and LAV and RAV measurements were subsequently indexed to body weight and compared among imaging modalities.

RESULTS

The LAV determined by echocardiographic methods did not differ significantly from the LAV determined by MDCT. However, the RAV determined by 3-D echocardiography and 2-D echocardiography via the left apical and left cranial windows differed significantly from the RAV determined by MDCT. Bland-Altman analyses indicated that the indexed LAV and RAV determined by echocardiographic methods were systematically underestimated, compared with MDCT measurements, but the bias was much smaller for LAV than for RAV.

CONCLUSIONS AND CLINICAL RELEVANCE

Results suggested that, for dogs, echocardiography might be an acceptable alternative to MDCT for measurement of LAV but not for measurement of RAV. However, the study population was small and homogenous in terms of breed, age, and weight. These findings need to be validated in a larger, more varied population of dogs with and without cardiac disease.
Odniesienia biblijne w twórczości polskich zespołów metalowych
Authors: Socha Krzysztof
Published: December 2019

Biblical references in the work of Polish metal bands One of the many factors which has been creating the west culture is the Judeo-Christiantradition based on the Bible. Motives, quotations and symbols that are coming from the Biblehave been permeating to the European languages as well as they have influenced both thehigh culture and the popular culture. Moreover, the biblical inspirations can be found in metalmusic as well. The underlying paper constitutes an attempt of brief characterization when itcomes to more original biblical motives that are constantly appearing in the lyrics of metalbands, simultaneously, it emphasizes a slight current interest of this biblical aspect in thescientific researches.
Djent – cyfrowa neomoderna metalu?
Authors: Mądro Andrzej
Published: December 2019

Djent - digital metal neomoderne?

Deep-rooted ideas of “art rock” do not expire and recently gave rise to the new, progressive metal trends like djent. It wants to be the elite, the modern, and the innovative. At the expense of greater commercial success, djent bands cut off from the mainstream and operate independently, incessantly exceeding stylistic and aesthetical boundaries. Poetics of their music often reveal a tension between elitism and egalitarianism, intellect and corporeality, individuality and convention. But can it be seen as some kind of musical trans-avant-garde or neomodernistic?
The poet who failed his best play? Podmiot mówiący w utworach zespołu Nightwish wobec paradygmatu romantycznego
Authors: Svetlova Anna
Published: December 2019

The poet who failed his best play? Speaking subject in the tracks of Nightwish in the face of the romantic paradigm

The article gives an insight into the problem of lyrical subject in songs recorded by Nightwish, along with the myth of the damned poet led by the group’s leader, Tuomas Holopainen, and romantic concepts of poetry, love and nature. It raises issues of autobiography and authenticity of artists, as well as the multidisciplinary narrative in rock music led on literary, iconic and musical levels. This text focuses primarily on the early Nightwish songs, the Century Child concept album and the End of an Era concert as the last live performance of Nightwish with Tarja Turunen as the lead singer.
Wykorzystanie technologii wirtualnej rzeczywistości w psychologii klinicznej
Authors: Cyranek Łucja
Published: December 2019

Employing VR technology in clinical psychology
Abstract
This paper will analyse the usage of virtual reality (VR) technology in clinical psychology especially neuropsychology. VR has emerged few years ago as a potentially efficient method of providing general and specialty psychological health. Clinical psychologist and neuropsychologists use VR in diagnosis, therapy and rehabilitation for example of anxiety disorders and executive functions. One of the most important advantage of using virtual reality tasks in neuropsychology is high ecological validity which is necessary for providing natural contects of diagnosis. The analysis is based on recent works of American researchers who used virtual reality methods like Nonimmersive Virtual Coffe Task, Virtual Supermarket Task, Virtual Reality Lateralized Attention Test, Virtual Reality Shopping Task and Virtual Classroom Task.
Etyczne aspekty prowadzenia terapii online: analiza wybranych stron internetowych
Authors: Rudnicka Patrycja , Pindych Anna , Probierz Eryka
Published: December 2019

Ethical dimensions of conducting online therapy: the analysis of selected websites
Abstract
The development of online psychological counselling brings new ethical challenges, related to the use of advanced technologies in traditional psychological tasks. The aim of the article is to present two studies of websites dedicated to provide online psychological counselling services (N = 43, 80), in which the degree of implementation of ethical principles was verified. The results confirmed that not all ethical requirements are implemented to a sufficient degree. The article also presents proposals for actions aimed at increasing ethical awareness of practitioners and changes necessary to introduce in Polish codes of ethic.
Fenomen ruchu antyszczepionkowego w cyberprzestrzeni, czyli fake news i postprawda na usługach hipotezy Andrew Wakefielda
Authors: Demczuk Agnieszka
Published: December 2019

The phenomenon of anti-vaccination movement in cyberspace, or fake news and post-truth in the service of Andrew Wakefield hypothesis
Abstract
The opponents of vaccinations have been expressing their concerns about the undesirable effects of vaccinations for more than two hundred years. They are guided by religious and ideological reasons and refuse to immunize themselves and children. They argue that the obligation to vaccinate is a limitation of their human rights. The vaccination movement has been present in the social sphere since the 19th century, however after the publication of Andrew Wakefield’s article on the subject of the alleged connection between vaccination and autism – the movement became very popular and contributed to a significant increase in the number of unvaccinated children in some countries. Nowadays, in the cyberspace and mass communication, it seems that both medical and social sciences face new challenges related to the spread of the movement. Hate speech, fake news and disinformation present in cyberspace strengthen and consolidate anti-vaccine attitudes. The phenomenon of Facebook means that every information can be made available to other users a few thousand times – even the untrue and misleading
Sand Dune Streptomyces JB66 Native to the Great Indian Thar Desert Inhibits Multidrug-Resistant Pathogens
Authors: Begani Jyotsna , Lakhani Jyoti , Harwani Dharmesh
Published: December 2019

The efficacy of an actinomycete strain JB66 recovered from a sand dune soil from the Bikaner district of the Thar desert in inhibiting the growth of various bacterial pathogens was studied. The type strains Staphylococcus aureus, Shigella flexneri, Klebsiella pneumoniae and the clinical isolates Escherichia coli, multidrug-resistant S. aureus and P. vulgaris were included in the antimicrobial assays. Polyphasic characterization of JB66 isolate revealed its identity as Streptomyces (MH762010). It showed 88.99-89.24% sequence similarity with the other members of this genus and share the maximum (88.89%) similarity with Streptomyces sp. ATSC13. The strain JB66 was found to produce a high amount of extracellular L-asparaginase, catalase, gelatinase, protease, tyrosinase and urease enzymes. The partial chemical categorization of the methanolic crude extract of the JB66 strain led to the preliminary identification of various metabolic compounds. The thin-layer chromatography fractionation revealed the presence of prodigiosin pigment or chandramycin, cephalosporin or zeatin, daidzein, demethoxy rapamycin, 4,6-dihydroxy-7-methoxyisoflavone, munumbicins and amiclenomycin like compounds. Bio-autography revealed that the metabolites localized at the Rf values of 0.40, 0.46 and 0.53 in TLC profile had the actual bioactive fractions. UV-VIS spectrum absorbance maxima at 288 nm revealed the presence of an aromatic nucleus.
Formulation and Evaluation of Controlled Release Maintenance Dose Loaded Niosomes of Anti-Hypertensive Drug
Authors: Chandani Makvana  , Sahoo Satyajit
Published: December 2019

The present study was aimed to formulate, comparatively evaluate and optimize multiple lipid drug carriers of valsartan for oral controlled release to overcome the problems associated with the drug such as bioavailability, to reduce the dosage regimen, half life and to determine the appropriateness of niosomal formulation as a drug carrier. Ether injection method was chosen for the formulation of physically and chemically stable niosomes of valsartan. The formulation and process parameters were optimized by manufacturing placebo niosomes. Than drug loaded niosome was prepared by varying the concentration of span 60. The prepared nine formulations were evaluated for various parameters. Placebo niosomes were evaluated for appearance, odour, texture, creaming volume, pH and changes after 15 days. The medicated nine formulations were evaluated for organoleptic properties (appearance/color, odour), pH, total drug content, entrapment efficiency, mean particle size and polydispersibility index, zeta potential and In-vitro drug release. All formulations were off-white in color, odourless, and fluid in nature. It was stable and did not show sedimentation. The pH was found to be in the range of 4.6-5.4. Drug content was found in the range of 89.13 to 99.52. The Entrapment efficiency was found in range of 79.05 to 98.24. The mean vesicle size of drug loaded niosomes of the different batches ranged between 2.52-3.42μm. The polydispersvity index was in the range of 0.325 to 0.420 which indicates a narrow vesicle size distribution. The values of zeta potential were in the range of -20.29 mV to -30.55 mV which indicates that niosome had sufficient charge and mobility to inhibit aggregation of vesicles. All the nine formulations shows constant drug release in controlled manner up to 24 h. Formulation V7 was considered to be the best formulation as the % drug content (99.52 ± 0.97), % entrapment efficiency (98.24 ± 1.50) and % drug release at the end of 24th h (98.55) were high for V7. The optimized formulation V7 showed higher degree of correlation coefficient (r2) 0.9805 which indicates process of constant drug release from dosage form. The present study concludes that the prepared niosome is a convenient and efficiency carrier for the delivery of antihypertensive drug. Besides this, it provided controlled delivery of drug.
To Study the Significance of Processing Variables Using Quality by Design for Optimization of Nanoparticulate System of Cilnidipine
Authors: Bagada Arti , Vadalia K. R. , Raval M. K. , Gadhia Dolly
Published: December 2019

This investigation aimed to prepare Cilnidipine Nanoparticles by nanoprecipitation ultrasonication method and to study the significance of processing variables by applying quality by design. Cilnidipine is fourth-generation dual L/N-type Ca2+ channel blocker used for the management of hypertension. It is BCS class-II drug exhibiting lower aqueous solubility, which tends to lower bioavailability. The combination of Poloxamer 188 and Tween 80 was used as a stabilizer. The design of the experiment is one of the tools of Quality by design. Plackett -Burman design was applied for the screening of processing variables, which are significant for the method. The processing variables screened were stirring speed, antisolvent ratio, drug concentration, polymer concentration, stabilizer concentration. The effect of each parameter evaluated by particle size, entrapment efficiency, and drug release at 10 minutes of prepared Nanoparticles of Cilnidipine. Analysis of variance and Pareto-plot of Plackett-Burman design were utilized to find the significance of the factor and extent of the effect. The surface morphology of Cilnidipine Nanoparticles was studied by SEM. The Pareto plot, as well as statistical analysis of design, had shown that the Concentration of drug, solvent: antisolvent ratio and concentration of poloxamer 188 were the significant parameters for the method. The stabilizer concentration, the stirring speed, and the antisolvent ratio had a negative effect of while the concentration of drug has a positive effect on the particle size of Nanoparticles and drug release at 10 minutes and positive effect of entrapment efficiency of Cilnidipine Nanoparticles. The Cilnidipine Nanoparticles were characterized by FTIR and DSC analysis.
Development of HPLC Method for Stress Testing of Combination of Two Drugs Using Design of Experiment Concept
Authors: Bajaj Mohini , Nanda Sanju
Published: December 2019

This study was conducted to develop, an High Performance Liquid Chromatography using photodiode array detector (HPLC-PDA) method to analyse the samples generated by the stress testing of antifilarial combination (albendazole and diethylcarbamazine citrate) in the solution state. The concept of Quality by Design (Design of Experiment, DoE) approach was used for the development. For the separation of the drugs and its degradation products (DPs), DoE was applied in two stages, i.e., primary parameter stage where factors having major effect were selected. This stage gives us CQA (Critical Quality Attribute) which along with minor factors affecting were varied to get the secondary design. For each of the stage a different design was selected; for primary stage IV optimal design (Response Surface Method) was selected whereas for secondary stage, Taguchi orthogonal array design was selected. The major primary parameters affecting the HPLC method as screened by preliminary studies were the buffer pH, organic modifier (methanol or acetonitrile), initial hold time (start of gradient) and gradient time. The primary stage was completed successfully. The results were compiled in form of resolution of peak from next peak and analysed by DoE. The process fixed the values for buffer pH (4.38), organic modifier (acetonitrile) and gradient time (30 min). The CQA from primary run was initial hold time. This parameter along with other parameters: initial and final concentration of organic modifier, buffer type (phosphate or acetate), buffer strength (mM) and oven temperature were further varied and samples withdrawn were analysed. The data of secondary design was compiled in the form of resolution (R), analysed by Design Expert and final value for secondary parameter for HPLC method were fixed. The resolution of the peaks for some secondary runs was sufficient reflecting some type of interaction between the drugs and/or degradation products.
Screening Ethanolic Extract of Aerva lanata for α-Amylase Inhibition and in vitro Uptake of Glucose in Adipose Tissue and Psoas Muscle of Male Sprague Dawley Rats
Authors: Saisree S. , Rao B. Sasibhusana , Sudhakara G. , Mallaiah P. , Saralakumari D.
Published: December 2019

The study was intended to investigate anti-diabetic efficacy of Aerva lanata by determining its α-amylase inhibition activity and in vitro uptake of glucose in adipose tissue and psoas muscle isolated from male Sprague Dawley (SD) rats. Aerva lanata is reported to have many traditional and Ayurvedic uses. Male SD rats (n=3) of 150 g were sacrificed and 250 mg of respective tissues were isolated for the study. Aerva lanata ethanolic extract (ALE) (5-20 mg/mL) showed 13.30 to 54.08% α-amylase inhibition activity. Glucose uptake studies in in vitro conditions were carried out in both adipose tissue and psoas muscle in different sets - tissue alone, tissue along with (Aerva lanata extract: 50µg, 100µg, 150µg, insulin: 25 mU/L, insulin: 50 mU/L and Aerva lanata extract: 50µg + insulin: 25 mU/L, Aerva lanata extract: 100µg + insulin: 25 mU/L, Aerva lanata extract: 150µg + insulin: 25 mU/L, Aerva lanata extract: 50µg + insulin: 50 mU/L, Aerva lanata extract: 100µg + insulin: 50 mU/L, Aerva lanata extract: 150µg + insulin: 50 mU/L). The rate of glucose uptake by insulin action in these tissues was stabilized by ethanolic extract of Aerva lanata and this shows synergetic activity of insulin and Aerva lanata.
Phyto-chemical screening of Root Extracts of Glycyrrhiza glabra by Spectroscopic Methods (UV-VIS Spectrophotometer, FTIR and HPLC)
Authors: Kumari Arti , Kumar Jainendra
Published: December 2019

Glycyrrhiza glabra, commonly known as licorice, has traditionally been used in various medicinal preparations as expectorant, antimicrobial, antiviral, anticancer, anti allergic agent. It has also prominent role in skin whitening and has anti-inflammatory and esterogenic properties. This study aims to the characterization of the bioactive constituent glabridin in plant extract of Glycyrrhiza glabra in ethanol, methanol and ethyl acetate using UV-VIS and FTIR and HPLC. A wavelength scan (200-600 nm) by UV-Vis spectrophotometer was performed and the characteristic peaks were recorded. The scan confirmed presence of glabridin with λmax at 281 nm with a small number of additional phytochemicals indicated by the extra peaks. FTIR spectrum of 62% pure glabridin was compared to the spectra shown by the three extracts. Presence of glabridin in all the three extracts was corroborated by HPLC analysis with the retention time of 4.05 minute. Glabridin was also evaluated for its antibacterial activity against Propionibacterium acne, causative agent of Acne vulgaris and found to have the detrimental effect at concentration 500, 1000 and 1500 ppm.
Expression Enhancement of Trastuzumab in CHO Cells Using Codon Optimization and Promoter Selection for Mammalian Expression Vector
Authors: Joshi Sharvari , Mangaonkar Kiran , Krishnan Archana
Published: December 2019

Trastuzumab is recombinant humanized monoclonal IgG antibody used in the treatment of metastatic breast cancer. It blocks the overexpression of tyrosine kinase Her 2 family receptors and downregulation of pathways. Genetic optimizations like gene optimization and expression vector optimization are the key elements to enhance monoclonal antibody expression. Expression vector optimization includes major parameters like selection of right promoters, poly A tail selection of genome integrating elements. Codon optimization is gene designing approach without affecting the amino acid sequence of the protein. Current research work involves development of codon optimization algorithm for trastuzumab heavy and light chain gene and comparative analysis of expression in different vectors.
Pharmacological Evaluation of Antidiabetic and Diuretic Activity of Hydro-alcoholic Extract of Anogeissus latifolia Bark
Authors: Vyas Bhupendra , Sarangdevot Y. S.
Published: December 2019

The objective of the present investigation was to study the antidiabetic and diuretic potential of Anogeissus latifolia (A. latifolia) bark in experimental rats. The A. latifolia bark was extracted with hydro-alcoholic solvent by cold extraction method. Acute toxicity study was performed according to OECD 425 guidelines for hydro-alcoholic extracts of A. latifolia bark (ALBE). The dose of 150 mg/kg p.o. and 300 mg/kg, p.o. of ALBE was selected for further studies. Animals were prepared diabetic by administration of alloxan (120 mg/kg, i.p.). The albino rats were divided in to five groups for oral glucose tolerance test (OGTT) and alloxan induced anti diabetic model with six animals in each group. Diabetic animals were treated with hydro-alcoholic extract of A. latifolia bark for 20 days. The blood glucose level was estimated according to standard procedures. Diuretic activity hydro-alcoholic extracts of A. latifolia was evaluated by Lipshitz method. The result shows that hydro-alcoholic extract from bark of Anogeissus latifolia 300 mg/kg (ALBE-II) shown significant hypoglycemic activity as compared to glibenclamide and diabetic group. The ALBE does not exhibit significant diuretic activity which is considered as positive marker in diabetic phenomena. Hence in present study extract of A. latifolia posses antidiabetic activity. This study may be benchmark in future to use of this drug scientifically.
A Fission Yeast Bioassay for Rapid Screening for Antiproliferative Agents from Bauhinia variegata and Leptadenia reticulata
Authors: Vyas P. A. , Braganza V. J.
Published: December 2019

The aim of the present study was to evaluate the antiproliferative activity of crude extracts from leaves of Bauhinia variegata and Leptadenia reticulata using fission yeast Schizosaccharomyces pombe as a model. The crude extracts of both plants were prepared by three experimental conditions (25°C, elevated temperature and ultrasound waves) and four solvent systems [Water, Methanol, Water: Methanol (1:1) and Chloroform: Methanol (1:1)].  MTT assay was performed for estimating the cell viability and the results were expressed as % growth inhibition. Overall based on the MTT assay performed on yeast cells, Water: Methanol, Methanol and Water extracts showed higher antiproliferative activity than the positive control (Paclitaxel) in a dose-dependent manner. For Bauhinia variegata and Leptadenia reticulata, methanol extract (25°C temperature & elevated temperature, respectively) exhibited highest inhibition which was approximately 80%. In conclusion, this study has indicated that Bauhinia variegata and Leptadenia reticulata extracts possess strong antiproliferative activity. Furthermore, it could act as a potential source for identifying new molecules with chemotherapeutic potential against cancer.
Study on Bioactive Compounds of Jania rubens against Methicillin and Vancomycin Resistant Staphylococcus aureus
Authors: Chenniyappan Sasikala , Durairaj Geetharamani , Evetha K
Published: December 2019

The study is planned to find the antimicrobial activity of the extract of Jania rubens and to isolate the bioactive compound against MRSA and VRSA. Jania rubens collected from Mandapam (Pudumadam) Coastal water, East coast of India and extracted with ethanol. Antibacterial activity of J. rubens was tested against gram positive, gram negative bacteria and drug resistant bacteria). The antibacterial activities were expressed as zone of inhibition, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) Identification of compounds from crude extract of J. rubens carried by column chromatography, thin layer chromatography and NMR analysis. Finally J. rubens could serve as useful source of new antibacterial agent.
The Conception of Trauma in Depicting the Battlefields In Wilfred Owen’s War Poetry
Authors: Dewali Taybat I. , Barany Lazgin K.
Published: December 2019

This study aims at finding out the attitudes of non-English majors’ students at University of Duhok towards English language andearning, in general, and at University of Duhok in particular and exploring the reasons behind their attitudes. The study also investigates the differences in the participants' attitudes based on their major, gender, and studying level. For this purpose, a questionnaire mainly adopted from Gardner's (2004) "Attitude/Motivation Test Battery" was distributed to (259) students from six colleges affiliated to University of Duhok. These six colleges were divided into two groups: Sciences (College of Veterinary, College of Engineering, and College of Sciences) and Humanities (College of Law and Political Sciences, College of Economics and Administration, and College of Basic Education). The findings have revealed that students under study have expressed positive attitudes towards both the English language itself as a language and learning it as a subject of study. The study also has shown that there were no significant differences in the students' attitudes towards the study's variables due to gender and studying level, however, a significant difference based on the students' major was found.
The Disabled People Virtual Communities in Social Media from The Perspective of Public Sphere Theory
Authors: Hasfi Nurul , Rahardjo Turnomo
Published: December 2019

Many researches view the internet as a  virtual public sphere or forum potential that provides a space for minority groups to voice their opinions.  This article identifies the role of social media in creating political public sphere for voters with disabilities in Indonesia during Presidential Election in 2019. By applying the normative values provided by Public Sphere Theory and Sphere Public Subaltern Theory, this article determines various potentials as well as stumbling blocks of the internet as a virtual public sphere for internet users.  Virtual ethnography method is used to determine the identity of virtual communities of disabled people on three most active social media platforms in Indonesia, namely Instagram, Facebook and Twitter. Virtual Ethnography enables researchers to conduct an online observation on virtual communities three months prior to  the 2019 Presidential Election. The data findings help the researchers draw conclusion  that social media has technically advocated the endeavor of the disabled people’s political rights equality, proven by the existence of disabled people’s virtual communities and the narratives of struggle for their political rights equality. However, the data has shown an inadequacy of disabled people’s virtual community to create dynamic inter-activities between its members  as this community has not been able to reflect a public sphere that is able to build public opinion  which  effectively influences public policy.
Exposing HIV-1 Env: Implications for therapeutic strategies
Authors: Finzi Andrés
Published: December 2019

The human immunodeficiency virus (HIV-1) envelope glycoprotein trimer (Env) is exposed on the surfaces of both virions and infected cells. Thus, Env is the principal target for neutralizing antibodies and antibodies able to mediate antibodydependent cellular cytotoxicity (ADCC). The HIV-1 Env is a flexible molecule known to exist in at least three different conformational states: states 1, 2 and 3. Before interacting with the primary receptor, CD4, Env preferentially adopts a compact, “closed” conformation (state 1) that is largely antibody-resistant. The CD4 binding “opens” Env increasing the vulnerability of infected cells to ADCC mediated by non-neutralizing antibodies, as these easily-elicited antibodies preferentially recognize epitopes exposed in the open conformational states (states 2/3). These antibodies include the anti-coreceptor binding site and the anti-cluster A families of antibodies that, in combination with small CD4-mimetic compounds, stabilize a new asymmetric Env conformation (state 2A) that is vulnerable to ADCC. Approaches aimed at stabilizing this “open” conformation represent new interventional approaches to fight HIV-1 infection.
The Vision Health Research Network and its commitment to the scholarly development of its trainees
Authors: Anchouche Sonia , Liu Jiaru , Vucetic Sara , Santerre Kim , Zhou Tianwei
Published: December 2019

The Vision Health Research Network (VHRN) is a provincial scientific organization that aims to improve the ocular health of patients across Quebec by supporting local research endeavors in vision health. The VHRN Student Committee, composed of 288 trainees with diverse backgrounds, has demonstrated its commitment to the scholarly development of its members by providing leadership opportunities, creating networking events, increasing visibility of researchers-in-training and encouraging professional advancement through educational workshops and funding programs. In this article, we review the contributions of the VHRN Student Committee and discuss its future projects.
PENGUATAN KELEMBAGAAN DAN DESTINASI DESA WISATA TAPAK TILAS SULTAN AGUNG, MANGUNAN, BANTUL
Authors: Lailam Tanto , Daru Murti Awang , Yunita Ani
Published: December 2019

Program pengabdian dengan skema Program Pengembangan Desa Mitra (PPDM) ini dilakukan untuk memperkuat kelembagaan pengelola desa wisata dan destinasi di Desa Wisata Tapak Tilas Sultan Agung, Mangunan. Tujuan program ini adalah menjadikan mitra sebagai model pusat pertumbuhan ekonomi kerakyatan dan kesejahteraan masyarakat berkelanjutan melalui pengembangan potensi desa wisata yang dipadukan dengan budaya dengan pendekatan community based tourism. Metode pelaksanaan dilakukan dengan: survei dan wawancara, focus group discussion, pelatihan, pendampingan, dan fasilitasi.Hasil Pengabdian berupa penguatan kelembagaan dan destinasi Desa Wisata Tapak Tilas Sultan Agung Mangunan dilakukan dengan melakukan pemetaan potensi unggulan daya tarik wisata melalui survei dan wawancara. Setelah dipetakan secara baik, tentu dilakukan penguatan berkaitan dengan kelembagaan dan sumber daya manusia pengelola desa wisata melalui berbagai pelatihan yang diselenggarakan, yaitu: pelatihan manajemen kelembagaan desa wisata dan community based tourism, pelatihan komunikasi pariwisata, komunikasi pemasaran danÂ  â€œbranding desa wisataâ€, pelatihan pelayanan prima desa wisata, pelatihan best practice dan studi banding pengelolaan desa wisata ke Desa Wisata Nglanggeran dan Desa Wisata Bleberan. Selain itu, dilakukan penguatan destinasi di Desa wisata Tapak Tilas Sultan Agung dilakukan dengan menambahkan wisata agro berupa pemberian bibit kopi, penyediaan papan informasi daya tarik wisata yang ditempatkan pada daya tarik wisata wayu goyang, dan fasilitasi legalisasi perkumpulan seni budaya dengan pembuatan akta notaris.Kata Kunci: Desa Wisata, Tapak Tilas Sultan Agung, Destinasi Wisata, Manajemen Kelembagaan.
WORKSHOP PELATIHAN KIMIA KOMPUTASI BAGI GURU DAN MAHASISWA DI MATARAM
Authors: Ananto Agus dwi
Published: December 2019

Kegiatan Telah dilaksanakan workshop penggunaan media berbantuan komputer bagi guru dan siswa serta mahasiswa terkait sebagai salah satu upaya yang bisa dilakukan untuk meningkatkan kualitas pemahaman dan pembelajaran ilmu kimia di SMKN 3 Mataram. Workshop tersebut dilakukan sebagai upaya melakukan inovasi dalam proses pembelajaran guna pemahaman kimia lebih lanjut. Proses pembelajaran dengan menggunakan media komputer dirasa mampu menjawab tantangan susahnya mempelajari kimia baik ditingkat sekolah maupun universitas. Kimia komputasi adalah salah satu cabang ilmu kimia yang menggunakan komputer sebagai perantaranya. Pemahaman sasaran kegiatan tentang struktur molekul suatu senyawa diharapkan menjadi lebih meningkat setelah mengikuti kegiatan ini. Kegiatan ini tidak hanya sekedar memberikan pemahaman saja bagi sasaran kegiatan, akan tetapi juga sasaran kegiatan memporoleh skill dalam penggunaan salah satu program kimia komputasi melalui kegiatan pelatihan yang disampaikan oleh pakar yang ahli dibidang kimia komputasi