bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of Antimicrobial Activity, Hemostatic Efficacy, Blood Coagulation Dynamics, and DNA Damage of Linen–Copper Composite Materials
Authors: Mrozińska Zdzisława , Świerczyńska Małgorzata , Juszczak Michał , Woźniak Katarzyna , Kudzin Marcin H.
Published: January 2025

This research examined the biochemical and microbiological characteristics of linen–copper (LI-Cu) composite materials, which were synthesized using magnetronsputtering techniques. The LI-Cu composites underwent comprehensive physicochemical and biological analyses. Physicochemical evaluations included elemental analysis (C, O, Cu), microscopic examination, and assessments of surface properties such as specific surface area and total pore volume. Biological evaluations encompassed microbiological tests and biochemical–hematological assessments, including the activated partial thromboplastin time (aPTT) and prothrombin time (PT). We determined the effect of LI-Cu materials on the viability and DNA damage in peripheral blood mononuclear (PBM) cells. Moreover, we studied the interactions of LI-Cu materials with plasmid DNA using a plasmid relaxation assay. The antimicrobial activity of LI-Cu composites was assessed using methodologies consistent with the EN ISO 20645:2006 and EN 14119:2005 standards. Specimens of the tested material were placed on inoculated agar plates containing representative microorganisms, and the extent of growth inhibition zones was measured. The results demonstrated that the modified materials exhibited antimicrobial activity against representative strains of Gram-positive and Gram-negative bacteria, as well as fungi. The results showed the cyto- and genotoxic properties of LI-Cu against PBM cells in a time- and power-dependent manner. Furthermore, the LI-Cu composite exhibited the potential for direct interaction with plasmid DNA.
Natural Protein Films from Textile Waste for Wound Healing and Wound Dressing Applications
Authors: Ottaviano Livia , Buoso Sara , Zamboni Roberto , Sotgiu Giovanna , Posati Tamara
Published: January 2025

In recent years, several studies have focused on the development of sustainable, biocompatible, and biodegradable films with potential applications in wound healing and wound dressing systems. Natural macromolecules, particularly proteins, have emerged as attractive alternatives to synthetic polymers due to their biocompatibility, biodegradability, low immunogenicity, and adaptability. Among these proteins, keratin, extracted from waste wool, and fibroin, derived from Bombyx mori cocoons, exhibit exceptional properties such as mechanical strength, cell adhesion capabilities, and suitability for various fabrication methods. These proteins can also be functionalized with antimicrobial, antioxidant, and anti-inflammatory compounds, making them highly versatile for biomedical applications. This review highlights the promising potential of keratin- and fibroin-based films as innovative platforms for wound healing, emphasizing their advantages and the prospects they offer in creating next-generation wound dressing devices.
Unmasking the Area Postrema on MRI: Utility of 3D FLAIR, 3D-T2, and 3D-DIR Sequences in a Case–Control Study
Authors: Lara-García Javier , Romo-Martínez Jessica , De-La-Cruz-Cisneros Jonathan Javier , Olvera-Olvera Marco Antonio , Márquez-Bejarano Luis Jesús
Published: January 2025

The area postrema (AP) is a key circumventricular organ involved in the regulation of autonomic functions. Accurate identification of the AP via MRI is essential in neuroimaging but it is challenging. This study evaluated 3D FSE Cube T2WI, 3D FSE Cube FLAIR, and 3D DIR sequences to improve AP detection in patients with and without multiple sclerosis (MS). A case–control study included 35 patients with MS and 35 with other non-demyelinating central nervous system diseases (ND-CNSD). MRI images were acquired employing 3D DIR, 3D FSE Cube FLAIR, and 3D FSE Cube T2WI sequences. The evaluation of AP was conducted using a 3-point scale. Statistical analysis was performed with the chi-square test used to assess group homogeneity and differences between sequences. No significant differences were found in the visualization of the AP between the MS and ND-CNSD groups across the sequences or planes. The AP was not visible in 27.6% of the 3D FSE Cube T2WI sequences, while it was visualized in 99% of the 3D FSE Cube FLAIR sequences and 100% of the 3D DIR sequences. The 3D DIR sequence showed superior performance in identifying the AP.
Impact of a Segmented-Scan Strategy on Residual Stress and Fit Accuracy of Dental Prostheses Fabricated via Laser-Beam Powder-Bed Fusion
Authors: Kobayashi Yoshio , Takaichi Atsushi , Kajima Yuka , Qu Wenrui , Wakabayashi Noriyuki
Published: January 2025

The laser-beam powder-bed fusion (PBF-LB) method enables the semi-automatic fabrication of complex three-dimensional structures, making it useful for dental prostheses. However, residual stress during fabrication can cause deformation. Herein, we applied the segmented-scan strategy to three-unit fixed dental prostheses (FDPs) and evaluated its effects on residual stress and fit accuracy compared to conventional methods. Three-unit FDPs consisting of two abutments and a pontic were fabricated using a PBF-LB machine with Co-Cr-Mo powder. In the segmented-scan group, pontics and abutments were scanned separately to shorten the scan vector. Fit accuracy was assessed by measuring the gap between the abutment and the FDPs. Residual stress was measured in the X and Y directions at three points using X-ray diffraction, while CT scans were used to count internal microstructures. The residual stress was lower in the X-direction in the segmented-scan group (24.61–217.17 MPa, respectively) than in the control group (187.70–293.71 MPa, respectively). However, no significant differences in fit accuracy were observed (p < 0.05). The segmented-scan strategy reduced residual stress in the X-direction but did not improve the fit accuracy. Applying this strategy to dental prosthetic devices can shorten the scan vector and reduce residual stress.
Metal Powder Production by Atomization of Free-Falling Melt Streams Using Pulsed Gaseous Shock and Detonation Waves
Authors: Frolov Sergey M. , Ivanov Vladislav S. , Aksenov Viktor S. , Shamshin Igor O. , Frolov Fedor S. , Zangiev Alan E. , Eyvazova Tatiana I. , Popkova Vera Ya. , Grishin Maksim V. , Gatin Andrey K. , Dudareva Tatiana V.
Published: January 2025

A new method of producing metal powders for additive manufacturing by the atomization of free-falling melt streams using pulsed cross-flow gaseous shock or detonation waves is proposed. The method allows the control of shock/detonation wave intensity (from Mach number 4 to about 7), as well as the composition and temperature of the detonation products by choosing proper fuels and oxidizers. The method is implemented in laboratory and industrial setups and preliminarily tested for melts of three materials, namely zinc, aluminum alloy AlMg5, and stainless steel AISI 304, possessing significantly different properties in terms of density, surface tension, and viscosity. Pulsed shock and detonation waves used for the atomization of free-falling melt streams are generated by the pulsed detonation gun (PDG) operating on the stoichiometric mixture of liquid hydrocarbon fuel and gaseous oxygen. The analysis of solidified particles and particle size distribution in the powder is studied by sifting on sieves, optical microscopy, laser diffraction wet dispersion method (WDM), and atomic force microscopy (AFM). The operation process is visualized by a video camera. The minimal size of the powders obtained by the method is shown to be as low as 0.1 to 1 μm, while the maximum size of particles exceeds 400–800 μm. The latter is explained by the deficit of energy in the shock-induced cross-flow for the complete atomization of the melt stream, in particular dense and thick (8 mm) streams of the stainless-steel melt. The mass share of particles with a fraction of 0–10 μm can be at least 20%. The shape of the particles of the finest fractions (0–30 and 30–70 μm) is close to spherical (zinc, aluminum) or perfectly spherical (stainless steel). The shape of particles of coarser fractions (70–140 μm and larger) is more irregular. Zinc and aluminum powders contain agglomerates in the form of particles with fine satellites. The content of agglomerates in stainless-steel powders is very low. In general, the preliminary experiments show that the proposed method for the production of finely dispersed metal powders demonstrates potential in terms of powder characteristics.
On-Ship Trinocular Stereo Vision: An Experimental Study for Long-Range High-Accuracy Localization of Other Vessels
Authors: Yoshihara Kotaro , Yamamoto Shigehiro , Hashimoto Takeshi
Published: January 2025

Recently, several initiatives regarding maritime autonomous surface ships (MASSs) have been implemented worldwide. One of the fundamental technologies for attaining MASSs is the recognition and localization of surrounding ships. Traditional navigational instruments are inadequate for recognizing objects, and the authors investigated the potential of stereo vision. Conventional stereo camera systems are not suitable for localizing very distant objects. One proposed solution is to use an additional camera, thus using three-camera measurements of objects at long distances to reduce positional measurement errors, incorporating time-series averaging and keypoint-based techniques. This study evaluated experimentally the accuracy of measurements using three ship-mounted cameras. The accuracy and precision of stereo measurements depend on the distance between the camera positions, referred to as the baseline length. Conventional stereo cameras are typically used to measure objects at distances of up to 200 times the baseline length. This study indicates that, using trinocular stereo vision, a target ship at distances up to 2500 m, which is 500 times the baseline length, can be measured with an accuracy of approximately 5% of the RMSE.
Urban Park Planning for Sustainability: Resident Insights from China’s Major Cities
Authors: He Biao , Wang Siyu , Xiong Qian , Zhao Zheng , Hou Yilei
Published: January 2025

Urban parks are pivotal in the sustainable development of urban ecosystems, significantly enhancing the ecological environment and residents’ quality of life. To scientifically improve urban park management and ensure long-term sustainability, this study aims to establish a framework for understanding and predicting residents’ spatial perceptions in urban parks. Overcoming the constraints of traditional empirical research, this study conducted a questionnaire survey involving 701 residents from Beijing Chaoyang Park, Shanghai Century Park, and Guangzhou Yuexiu Park from May to July 2024. By combining the semantic differential (SD) method, importance–performance analysis (IPA), and cognitive map methods, the study holistically assessed residents’ spatial perceptions and their variations in urban parks. The SD analysis highlighted perceptional differences in the three urban parks, revealing the needs for tailored planning considering personal characteristics and geographical location, which is essential for enhancing park services and overall satisfaction. Comparative analysis showed significant variations in the preferences in different resident groups, with Chaoyang Park facing a sense of deprivation due to weaker natural features, suggesting the critical role of natural elements in park design, while Century and Yuexiu Parks were highly valued for aligning park design with local needs and preferences. Cognitive maps reveal residents’ spatial perception of urban parks, particularly their significant differences in familiarity with the natural and social functions, artificial landscapes, and internal attractions of parks, which informs the integration of design elements that cater to varying preferences and foster a stronger sense of place. This study confirms the effectiveness of combining traditional methods with spatial perception analysis for quantitatively evaluating residents’ spatial perception, and provides useful references for the sustainable planning and management of urban parks.
Exploring the Links Between Forest Stewardship Council Certification and Biodiversity Outcomes at a Global Level
Authors: Kadam Parag , Dwivedi Puneet
Published: January 2025

Forests contain just under 58% of all known species globally, with approximately 61% of these found in countries with Forest Stewardship Council (FSC)-certified forests. Few studies have directly analyzed the association between biodiversity and certification, often focusing on limited geographical or temporal scopes. There is a lack of understanding regarding the socio-political and ecological drivers of FSC certification’s relationship with biodiversity conservation. We assess the associations between country-level characteristics and the relationship between FSC’s forest management (FSC-FM) certification area and independently made biodiversity estimates from the International Union for Conservation of Nature (IUCN). Specifically, using generalized random forests (GRF) and data from 91 countries between 2008 and 2019, we examine the associated factors that govern FSC-FM certifications relationship with percentage threatened species. The results indicate that increasing FSC-FM-certified areas is linked to a reduction in the percentage of threatened species by 0.1 to 0.15. Moreover, FSC-FM certifications show a positive relationship with biodiversity in regions with high population densities and significant tree cover loss. Enhanced perception of corruption control and reduced extraction of non-forestry resources further strengthen this association. These findings provide the FSC with strategic insights to expand FM certification, contributing to biodiversity conservation through sustainable forest management.
Analysis of Vegetation Restoration Potential and Its Influencing Factors on the Loess Plateau: Based on the Potential Realization Model and Spatial Dubin Model
Authors: Wang Chao , Han Lili , He Youjun , Zhang Yu , Zhang Maomao
Published: January 2025

Improvements in vegetation coverage are driven by both resource endowment conditions and policy behaviors. To accurately reflect the vegetation restoration effect after ecological policies, this study used the potential realization model to calculate the potential realization degree of vegetation restoration on the Loess Plateau and to assess the vegetation restoration effect after the Grain for Green Program from 2000 to 2020. Then, the influencing factors were explored using the spatial Dubin model. The results reveal that (1) the EVI value of the Loess Plateau in northern Shaanxi increased from below 0.25 at the beginning of the study to approximately 0.35 by the end, indicating that the green territory of the Loess Plateau gradually expanded to the northwest over the study period, and that the east and west of the Loess Plateau are key areas of vegetation cover for further improvement; (2) compared to the traditional EVI indicator, the potential realization degree can more accurately evaluate the vegetation restoration effect driven by ecological policies; (3) policy intensity is positively correlated with the growth rate of the vegetation restoration potential realization degree by 0.183 and significant at 1% level, making it the primary factor influencing the effect of vegetation restoration. Additionally, annual average precipitation and annual sunshine percentage have significant spatial positive contributions to the improvement of vegetation restoration on the Loess Plateau. The study’s findings are expected to contribute to the development of a scientific basis for adjusting the vegetation restoration policy on the Loess Plateau and enhancing ecological restoration efforts.
Fatigue-Limit Assessment via Infrared Thermography for a High-Strength Steel
Authors: Zhao Yingxin , Lin Zhaodong , Xia Yu , Chen Liming , Gu Guoqing , Pan Like
Published: January 2025

Infrared thermography techniques have proven to be very effective for assessing the fatigue limits of metallic materials with obvious temperature variations. But for some materials, it has been shown that the temperature variation is very limited, and the accuracy of infrared thermographic techniques is not verified. In this study, the fatigue properties of a high-strength steel (SAE52100) were evaluated with traditional fatigue-loading techniques and infrared thermographic methods. The traditional fatigue experiments were loaded at a frequency of 80 Hz with a stress ratio of R = −1, and the fatigue limit at the fatigue lifetime of N = 107 cycles was about 800 MPa. Besides, three additional specimens were loaded with step-by-step increasing stress-loading amplitude, where the maximum temperature increments and temperature distribution were recorded via infrared thermographic techniques. The infrared detections revealed that the maximum value of the temperature increase was only about 1 °C. The fatigue limit was first evaluated based on the maximum temperature variation, then the prediction was refined based on fatigue intrinsic dissipation. The fatigue limits predicted with maximum temperature variation were shown to be 841 MPa, 772 MPa, and 787 MPa, respectively, while the fatigue limits predicted based on fatigue intrinsic dissipation were 793 MPa, 791 MPa, and 789 MPa. Finally, an FEM simulation of temperature variation during fatigue loading was implemented to verify the experimental results. This study provides a solid foundation for the applications of infrared thermography techniques for materials with lower energy dissipations.
Innovative Supported Membranes for Ion Mobility Spectrometer (IMS) Sample Introduction Systems with High Permeability Relative to Toxic Agents in Air (TAAs)
Authors: Wiśnik-Sawka Monika , Fabianowski Wojciech , Gajda Dorota
Published: January 2025

One of the main objectives of the ion mobility spectrometry (IMS) technique is to reduce moisture in detection systems, which causes the formation of ion clusters and ion water and a reduction in formed clusters’ activity. Thus, one of the methods limiting moisture in a sampling injection system is to use hydrophobic polymeric membranes. The use of membranes with high permeability relative to the analysed organic compounds is required, including toxic agents in air (TAAs). Such requirements align with those of polydimethylsiloxane (PDMS) membranes. Unfortunately, thin PDMS membranes are not mechanically resistant. In this study, relatively thin PDMS membranes were reinforced with fine mesh fabric supports. These supports were chemically modified with selected oligoglycol derivatives and finally coated with PDMS. The obtained membranes were tested for water permeability and TAA simulants.
Investigation of the Effect of the Shape of Cutting Knives Limiting Burr in High-Strength Multiphase Steel Sheets
Authors: Mróz Sebastian , Stefanik Andrzej , Szota Piotr , Galusińska Sabina , Zaława Dariusz , Adamiec Andrzej , Zaława Natalia
Published: January 2025

In this study, numerical modeling and experimental tests of the sheet metal cutting process were carried out in order to determine the shape of the cutting knives for a roller shear, ensuring the minimization of burr on the cut edge. A rolling mill was used for the tests, enabling the replication of the cutting process in a roller shear (demonstrating the possibility of using cutting rollers). The cutting edges of the sheets were examined using light microscopy and then compared with the results of numerical simulations to determine the cutting quality. The tests were performed for multiphase Complex Phase (CP) grade steel. The initial thicknesses of sheets were equal to 1 and 2 mm. Based on the results of theoretical research, four shapes of cutting rollers were designed, of which two shapes were selected for experimental tests. The analysis of the test results shows that the lowest burr values were obtained for straight and beveled rollers. Analyzing the size of burr obtained in experimental tests, it can be concluded that for each of the two variants of the roller shape, a reduction in burr was achieved. Greater reductions in burr were achieved for shaped (cut) rolls.
The Temperature-Dependent Tight Binding Theory Modelling of Strain and Composition Effects on the Electronic Structure of CdSe- and ZnSe-Based Core/Shell Quantum Dots
Authors: Malkoç Derya , Ünlü Hilmi
Published: January 2025

We propose a temperature-dependent optimization procedure for the second-nearest neighbor (2NN) sp3s* tight-binding (TB) theory parameters to calculate the effects of strain, structure dimensions, and alloy composition on the band structure of heterostructure spherical core/shell quantum dots (QDs). We integrate the thermoelastic theory of solids with the 2NN sp3s* TB theory to calculate the strain, core and shell dimensions, and composition effects on the band structure of binary/ternary CdSe/Cd(Zn)S and ZnSe/Zn(Cd)S QDs at any temperature. We show that the 2NN sp3s* TB theory with optimized parameters greatly improves the prediction of the energy dispersion curve at and in the vicinity of L and X symmetry points. We further used the optimized 2NN sp3s* TB parameters to calculate the strain, core and shell dimensions, and composition effects on the nanocrystal bandgaps of binary/ternary CdSe/Cd(Zn)S and ZnSe/Zn(Cd)S core/shell QDs. We conclude that the 2NN sp3s* TB theory provides remarkable agreement with the measured nanocrystal bandgaps of CdSe/Cd(Zn)S and ZnSe/Zn(Cd)S QDs and accurately reproduces the energy dispersion curves of the electronic band structure at any temperature. We believe that the proposed optimization procedure makes the 2NN sp3s* TB theory reliable and accurate in the modeling of core/shell QDs for nanoscale devices.
Investigation of Biodegradation, Artificial Aging and Antibacterial Properties of Poly(Butylene Succinate) Biocomposites with Onion Peels and Wheat Bran
Authors: Sasimowski Emil , Grochowicz Marta , Janczak Katarzyna , Nurzyńska Aleksandra , Belcarz-Romaniuk Anna
Published: January 2025

The present article focuses on the characterization of the new biocomposites of poly(butylene succinate) (PBS) with fillers of plant origin such as onion peels (OP) and durum wheat bran WB (Triricum durum) subjected to composting and artificial aging. The susceptibility to fungal growth, cytotoxicity and antibacterial properties were also examined. The biodegradation of the samples was investigated under normalized conditions simulating an intensive aerobic composting process. It was shown that the tested natural fillers significantly accelerate the biodegradation process of the composition (after 90 days mass loss of PBS 7%) and that the samples with WB degrade much faster (corresponding mass loss 86%) than those containing OP (corresponding mass loss 21%). The remains of the samples after composting were subjected to chemical structure analysis (FTIR), and their thermal properties were determined using differential scanning calorimetry (DSC). It was shown that the degree of crystallinity of PBS and composites increased with the increasing time of composting. In the case of pure PBS, this increase was a maximum of 31.5%, for biocomposite with OP 31.1% and for those containing WB 21.2%. FTIR results showed that cleavage of polymer chains by hydrolysis took place during composting. The tested samples were also subjected to artificial aging under conditions simulating solar radiation and were sprayed with water. After artificial aging, the significant changes in the color of the samples as well as the porosity of their surface was noted, which was mainly due to the effect of photodegradation of both the used OP and WB fillers. Additionally, FTIR analysis indicated that samples were degraded by photooxidation processes. The ability of fungi to grow on the surface of the samples was also tested. The results demonstrate the possibility of using the developed biocomposite materials as a carbon source for the growth of fungi. The antibacterial tests showed that samples containing OP exhibited strong antibacterial properties regardless of their wt.% content. Additionally, a cytotoxicity test was performed on a BJ cell line, demonstrating that none of the tested biocomposites were cytotoxic. Moreover, those with the addition of WB statistically significantly supported the viability of both fibroblast and bacteria cells, showing their biological safety but lack of antibacterial activity.
Theoretical Research on the Axial Compression Capacity of Reinforced Concrete Mid-Long Columns Reinforced with Ultra-High-Performance Concrete
Authors: Tong Xiaolong , Liao Zhengwu , Liu Wei , Guo Huajing , Wu Jianliang
Published: January 2025

Ultra-high-performance concrete (UHPC) is a cement-based composite material characterised by exceptional strength, low porosity and high durability, making it highly promising for reinforcement engineering. Based on the theory of tangential modulus, a calculation method has been developed for the axial compression capacity of reinforced concrete (RC) medium and long columns strengthened with UHPC, using the constitutive relation of materials and internal and external force balance conditions. This study analysed the influence of UHPC reinforcement layer thickness, reinforced layer, reinforcement ratio, column slenderness ratio and initial load level of core columns on the bearing capacity of reinforced columns. The results indicated that the bearing capacity of the mid-long columns increases with the thickness of the UHPC reinforcement layer and its reinforcement ratio. In addition, the bearing capacity decreases with the increase in the column slenderness ratio and initial load level of the core column.
Influence of the Thermoplastic Fiber Ratio on the Mechanical Properties of Recycled Carbon Fibers During the Carding Process
Authors: Ivars Jean , Labanieh Ahmad Rashed , Soulat Damien
Published: January 2025

This study investigates the impact of carding and blending recycled carbon fibers (rCF) with crimped thermoplastic polypropylene (PP) fibers on the mechanical properties of rCF, using a Weibull statistical approach. Tensile properties of rCF were evaluated before and after carding with varying rCF/PP blend ratios (100/0%, 85/15%, 70/30%, and 50/50%). A comparison between the two-parameter and three-parameter Weibull models showed that the two-parameter model provided a better fit for rCF properties before carding. The results show that adding crimped PP fibers during carding helps to decrease the stress-at-break disparity and move their distribution to higher values. Furthermore, a slight increase in tensile modulus was observed in carded rCF, with higher PP ratios associated with smaller scatter modulus distributions. Elongation at break remained consistent, with the Weibull modulus increasing slightly with carding and the inclusion of PP fibers, indicating improved consistency. Overall, carding rCF with PP fibers helped in the mechanical property uniformity of the resulting carded webs without compromising tensile performance. This work shows the potential of the carding process with or without thermoplastic fibers to efficiently realign and give continuity to discontinuous recycled carbon fibers.
Incorporation of Histogram Intersection and Semantic Information into Non-Negative Local Laplacian Sparse Coding for Image Classification
Authors: Shi Ying , Wan Yuan , Wang Xinjian , Li Huanhuan
Published: January 2025

Traditional sparse coding has proven to be an effective method for image feature representation in recent years, yielding promising results in image classification. However, it faces several challenges, such as sensitivity to feature variations, code instability, and inadequate distance measures. Additionally, image representation and classification often operate independently, potentially resulting in the loss of semantic relationships. To address these issues, a new method is proposed, called Histogram intersection and Semantic information-based Non-negativity Local Laplacian Sparse Coding (HS-NLLSC) for image classification. This method integrates Non-negativity and Locality into Laplacian Sparse Coding (NLLSC) optimisation, enhancing coding stability and ensuring that similar features are encoded into similar codewords. In addition, histogram intersection is introduced to redefine the distance between feature vectors and codebooks, effectively preserving their similarity. By comprehensively considering both the processes of image representation and classification, more semantic information is retained, thereby leading to a more effective image representation. Finally, a multi-class linear Support Vector Machine (SVM) is employed for image classification. Experimental results on four standard and three maritime image datasets demonstrate superior performance compared to the previous six algorithms. Specifically, the classification accuracy of our approach improved by 5% to 19% compared to the previous six methods. This research provides valuable insights for various stakeholders in selecting the most suitable method for specific circumstances.
A Structural Bioinformatics-Guided Study of Adenosine Triphosphate-Binding Cassette (ABC) Transporters and Their Substrates
Authors: Younus Iqra , Ford Robert C. , Prince Stephen M.
Published: January 2025

Adenosine triphosphate-binding cassette (ABC) transporters form a ubiquitous superfamily of integral membrane proteins involved in the translocation of substrates across membranes. Human ABC transporters are closely linked to the pathogenesis of diseases such as cancer, metabolic diseases, and Alzheimer’s disease. In this study, four ABC transporters were chosen based on (I) their importance in humans and (II) their score in a structural bioinformatics screen aimed at the prediction of crystallisation propensity. The top-scoring ABC transporters’ orthologs (Mus musculus—mouse ABCB5, Ailuropoda melanoleuca—giant panda ABCB6, Myotis lucifugus—little brown bat ABCG1 and Mus musculus ABCG4) were then expressed in Saccharomyces cerevisiae with a combined green fluorescent protein and polyhistidine tag, enabling visualisation and purification. After partial purification and in the presence of the detergent (n-dodecyl-β-D-maltoside), the kinetic parameters of the ATP hydrolysis reactions of the orthologs were determined, as well as the extent of stimulation of their activity when presented with putative substrates. We discuss the efficiency of such bioinformatics approaches and make suggestions for their improvement and wider application in membrane protein-structure determination.
Industrial Sludge Valorization in Soil Application
Authors: Trpčevská Jarmila , Maruškinová Gréta , Laubertová Martina , Kundráková Klaudia , Oráč Dušan
Published: January 2025

Sludges produced in the pickling line in a steel company were applied to the soil as a secondary source of nutrients. In the experiments, the addition of two types of sludge, namely neutralization sludge (NS) and phosphatization sludge (PS), was evaluated. The listed sludges are defined as non-hazardous waste and contain elements such as iron, phosphorus, zinc, and others in minor quantities. The primary motivation for using these sludges in the soil is their phosphorus content, as phosphorus is a crucial mineral nutrient. Leaf lettuce was used as a test object. The quality of the soil in which it grew can be seen from the appearance of its leaves. The period of monitoring the growth of leaf lettuce was 35 days, while the length of the leaves was measured on different days (Days 7, 14, 21, 28, and 35). Different ratios of sludge to soil were chosen (1:2, 1:1, and 2:1) for the experiments. The comparative sample for evaluation of sludge addition was leaf lettuce that grew in soil without any presence of the sludge. The application of NS to the soil in all proportions significantly accelerated the growth of the lettuce, whereas the application of PS to the soil had a negative effect on the growth. Various analytical techniques, such as SEM, EDX, XRD, HR CS FAAS, and leachability tests, were used to analyze samples of the sludges.
Comparison of Measurement Protocols for Internal Channels of Transparent Microfluidic Devices
Authors: Kaal Joris , Feltin Nicolas , Lelong Marc , Yin Huabing , Glidle Andrew , Romieu Kevin , Batista Elsa
Published: January 2025

The microfluidic industry faces a significant challenge due to the lack of sensitive and standardized methods. One critical need is the measurement of internal channel dimensions in fully assembled chips. This study presents and compares several protocols for measuring these dimensions, including optical profilometry, optical microscopy, and tiled digital imagery. Standardized chips made from two materials commonly used in microfluidics (borosilicate glass and Cyclic Olefin Copolymer) were evaluated using each protocol. A consistency analysis using normalized error statistics identified optical profilometry as the most reliable method, offering the lowest uncertainty and the highest consistency with nominal geometry values. However, all protocols encountered difficulties with vertical depth measurements of internal structures. Future research should focus on addressing these limitations, including investigating the influence of multiple refractive surfaces on optical profilometry and exploring confocal microscopy. In conclusion, this work provides a comprehensive comparison of measurement protocols for internal microfluidic structures and offers a practical solution for applications in the microfluidic industry, while also identifying important directions for future research.
Research on Dry Coupling Technology in the Ultrasonic Non-Destructive Testing of Concrete
Authors: Li Jun , Chen Zeyu
Published: January 2025

In the health monitoring and safety assessments of concrete structures, ultrasonic non-destructive testing (NDT) technology has become an indispensable tool due to its non-destructive nature, efficiency, and precision. However, when used in inspecting irregular concrete surfaces, traditional planar ultrasonic transducers often encounter energy loss and signal attenuation induced by poor interface coupling, which significantly reduces the accuracy and reliability of the test results. To address this problem, this article proposes a point-contact dry coupling ultrasonic transducer solution, which enables efficient acquisition of ultrasonic signals within concrete without the need for couplants. By combining an array imaging system with a total focusing algorithm, this study not only significantly enhances the convenience and signal-to-noise ratio (SNR) of concrete ultrasonic imaging, but also opens new pathways for ultrasonic NDT technology in concrete.
Probiotics in the Management of Chronic Bacterial Prostatitis Patients: A Randomized, Double-Blind Trial to Evaluate a Possible Link Between Gut Microbiota Restoring and Symptom Relief
Authors: Vocca Cristina , Abrego-Guandique Diana Marisol , Cione Erika , Rania Vincenzo , Marcianò Gianmarco , Palleria Caterina , Catarisano Luca , Colosimo Manuela , La Cava Gregorio , Palumbo Italo Michele , De Sarro Giovambattista , Ceccato Tommaso , Botti Simone , Cai Tommaso , Palmieri Alessandro , Gallelli Luca
Published: January 2025

Several studies have suggested that probiotics could play a role in the management of patients with chronic bacterial prostatitis (CBP). In this randomized, placebo-controlled clinical study, we evaluated the efficacy and safety of consumption of probiotics containing human Lactobacillus casei DG® as an add-on treatment in patients with clinical recurrences of CBP, through gut microbiota modification analysis. Enrolled patients with CBP were randomized to receive for 3 months probiotics containing human Lactobacillus casei DG® or placebo following 1 month treatment with ciprofloxacin. During the enrollment and follow-ups, urological examinations analyzed symptoms and quality of life, while microbiological tests analyzed gut and seminal microbiota. During the study, the development of adverse drug reactions was evaluated through the Naranjo scale. Twenty-four patients with CBP were recruited and treated for 3 months with placebo (n. 12) or with Lactobacillus casei DG® (n. 12). Lactobacillus casei DG® induced a significantly (p < 0.01) faster recovery of symptoms than placebo (2 days vs. 8 days) and an increased time free from symptoms (86 days vs. 42 days) without the occurrence of adverse events. In the probiotic group, the appearance of Lactobacilli after 30 days (T1) was higher vs. the placebo group, and a significant reduction in Corynebacterium, Peptoniphilus, Pseudomonas, Veillonella, Staphylococcus, and Streptococcus was also observed. These preliminary data suggest that in patients with CBP, the use of Lactobacillus casei DG after an antimicrobial treatment improves the days free of symptoms and the quality of life, without the development of adverse drug reactions.
Biological and Health-Promoting Potential of Fruits from Three Cold-Hardy Actinidia Species
Authors: Latocha Piotr , Silva Ana Margarida , Moreira Manuela M. , Delerue-Matos Cristina , Rodrigues Francisca
Published: January 2025

Fruits are essential components of the human diet, valued for their diverse bioactive compounds with potential health-promoting properties. This study focuses on three cold-hardy Actinidia species, namely A. arguta, A. kolomikta, and A. polygama, examining their polyphenolic content, antioxidant/antiradical activities, scavenging capacity and effects on intestinal cell viability (Caco-2 and HT29-MTX). A comprehensive profile of their phenolic compounds was identified, in descending order of total polyphenol content: A. kolomikta > A. arguta > A. polygama. Across species, 16 phenolic acids, 2 flavanols, 2 flavanones, 11 flavonols, and 3 flavones were quantified, with caffeine as a prominent compound. A. kolomikta achieved the highest antioxidant activity, with ‘Vitakola’ cultivar showing almost double the antioxidant activity compared to ‘Tallinn’ and ‘Pozdni’. By contrast, A. arguta ‘Geneva’ and A. polygama ‘Pomarancheva’ exhibited significantly lower activity in both FRAP and DPPH assays. Notably, A. kolomikta cultivars showed distinct radical-scavenging capacities, particularly for superoxide, wherein ‘Tallinn’ and ‘Pozdni’ achieved the highest values. Cell viability tests on Caco-2 and HT29-MTX cells revealed a dose-dependent reduction in viability, notably stronger in Caco-2 cells. Overall, this study underscores the therapeutic potential of Actinidia species.
Dual-Mechanism Study of Metal-Free g-C3N4 Catalysts for Advanced Oxidation Under Non-Photocatalytic Conditions
Authors: Yang Zixu , Sun Yang , Wang Weizhi , Yuan Xiaohan , Tian Pengfei , Xu Jing
Published: January 2025

Metal-free materials have been proved to be promising replacements of traditional metal-based catalysts for advanced oxidation reactions. Carbon nitride was found to be able to activate H2O2 and generate hydroxyl radicals (•OH). Nevertheless, the performance of carbon nitride is highly dependent on an external light source. In this work, we report a light-independent, metal-free catalyst based on g-C3N4 prepared using a facile calcination method. It is revealed that two reaction pathways, a radical (•OH) one and a nonradical (H2O2) one, coexist in organics oxidation on g-C3N4. The dominant reaction pathway is dependent on the condensation temperature of UCN. In addition, this g-C3N4 exhibited excellent stability after being recycled and reused for five cycles. The findings in this work can be used for the design of efficient and robust metal-free catalysts with both superior catalytic performance and high stability for various heterogeneous catalytic processes.
Development of Homogeneous Carboxylation of Phenolates via Kolbe–Schmitt Reaction
Authors: Merzliakov Dmitry A. , Alexeev Michael S. , Topchiy Maxim A. , Yakhvarov Dmitry G. , Kuznetsov Nikolai Yu. , Maximov Anton L. , Beletskaya Irina P.
Published: January 2025

In this study, the homogeneous carboxylation of potassium, sodium, and lithium phenolates in DMSO solution at 100 °C by the Kolbe–Schmitt reaction was investigated. The impact of water, phenolate concentration, and cation nature on the yield of products and reaction selectivity was demonstrated. Based on the patterns observed, it was concluded that a complex cluster mechanism governs the carboxylation reaction in the solution. The use of a homogeneous reaction medium allowed for convenient testing of various additives to assess their impact on the reaction. Basic additives such as sodium salts of mesitol, tert-butylcalix[4]arene, sodium isopropyl, and tert-butyl cabonates were found to enhance the reaction, increasing the yield of hydroxybenzoic acids by 20% (to 61.6%). The main product in the DMSO solution was identified as 4-hydroxybenzoic acid, in contrast to the classical Kolbe–Schmitt method which typically yields 2-hydroxybenzoic (salicylic) acid. The use of 13C NMR spectroscopy enabled the observation of a “carbonate complex” in the solution for the first time, with the carbonate carbon displaying a chemical shift value of 142 ppm, an unusual finding for stable carbonates, and located between the signals of free dissolved CO2 and carboxylate derivatives.
Unraveling TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR Transcription Factors in Safflower: A Blueprint for Stress Resilience and Metabolic Regulation
Authors: Yu Lili , Ma Xintong , Dai Mingran , Chang Yue , Wang Nan , Zhang Jian , Zhang Min , Yao Na , Umar Abdul Wakeel , Liu Xiuming
Published: January 2025

Safflower (Carthamus tinctorius L.), a versatile medicinal and economic crop, harbors untapped genetic resources essential for stress resilience and metabolic regulation. The TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR (TCP) transcription factors, exclusive to plants, are pivotal in orchestrating growth, development, and stress responses, yet their roles in safflower remain unexplored. Here, we report the comprehensive identification and characterization of 26 safflower TCP genes (CtTCPs), categorized into Class I (PROLIFERATING CELL FACTOR, PCF) and Class II (CINCINNATA and TEOSINTE BRANCHED1/CYCLOIDEA, CIN and CYC/TB1) subfamilies. Comparative phylogenetics, conserved motif, and gene structure analyses revealed a high degree of evolutionary conservation and functional divergence within the gene family. Promoter analyses uncovered light-, hormone-, and stress-responsive cis-elements, underscoring their regulatory potential. Functional insights from qRT-PCR analyses demonstrated dynamic CtTCP expression under abiotic stresses, including abscisic acid (ABA), Methyl Jasmonate (MeJA), Cold, and ultraviolet radiation b (UV-B) treatments. Notably, ABA stress triggered a significant increase in flavonoid accumulation, correlated with the upregulation of key flavonoid biosynthesis genes and select CtTCPs. These findings illuminate the complex regulatory networks underlying safflower’s abiotic stress responses and secondary metabolism, offering a molecular framework to enhance crop resilience and metabolic engineering for sustainable agriculture
Transcriptome Analysis of Stephania yunnanensis and Functional Validation of CYP80s Involved in Benzylisoquinoline Alkaloid Biosynthesis
Authors: Shi Wenlong , Li Qishuang , Li Xinyi , Luo Linglong , Gan Jingyi , Ma Ying , Wang Jian , Chen Tong , Zhang Yifeng , Su Ping , Ma Xiaohui , Guo Juan , Huang Luqi
Published: January 2025

The medicinal plant Stephania yunnanensis is rich in aporphine alkaloids, a type of benzylisoquinoline alkaloid (BIA), with aporphine being the representative and most abundant compound, but our understanding of the biosynthesis of BIAs in this plant has been relatively limited. Previous research reported the genome of S. yunnanensis and preliminarily identified the norcoclaurine synthase (NCS), which is involved in the early stages of the BIA biosynthetic pathways. However, the key genes promoting the formation of the aporphine skeleton have not yet been reported. In this study, based on the differences in the content of crebanine and several other BIAs in different tissues, we conducted transcriptome sequencing of roots, stems, and leaves. We then identified candidate genes through functional annotation and sequence alignment and further analyzed them in combination with the genome. Based on this analysis, we identified three CYP80 enzymes (SyCYP80Q5-1, SyCYP80Q5-3, and SyCYP80G6), which exhibited different activities toward (S)- and (R)-configured substrates in S. yunnanensis and demonstrated strict stereoselectivity enroute to aporphine. This study provides metabolomic and transcriptomic information on the biosynthesis of BIAs in S. yunnanensis, offers valuable insights into the elucidation of BIA biosynthesis, and lays the foundation for the complete analysis of pathways for more aporphine alkaloids.
Comparison of Aging Performances and Mechanisms: Super-Durable Fire-Resistant “Xuan Paper” Versus Chinese Traditional Xuan Paper
Authors: Dong Li-Ying , Zhu Ying-Jie , Wu Jin , Yu Han-Ping
Published: January 2025

Paper is a thin nonwoven material made from cellulose fibers as the main raw material together with some additives. Paper is highly flammable, leading to the destruction of countless precious ancient books, documents, and art works in fire disasters. In recent years, researchers have made a lot of efforts in order to obtain more durable and fire-retardant paper. Owing to the successful synthesis of ultralong hydroxyapatite (HAP) nanowires as a new kind of inorganic nanofiber material, it becomes possible to develop a new kind of super-durable and fire-resistant paper. Recently, the authors’ research group prepared a new kind of fire-resistant “Xuan paper” consisting of ultralong HAP nanowires. In this article, the super-durable fire-resistant “Xuan paper” based on ultralong HAP nanowires and the traditional Xuan paper based on cellulose fibers were evaluated by the accelerated aging method for 1200 days at 105 °C in air, which is the equivalent of 10,000 years of natural aging in the ambient environment. The aging mechanism of the traditional Xuan paper was further investigated by studying the fiber length/width and their distributions, morphology, infrared spectroscopy, thermogravimetric analysis, H–nuclear magnetic resonance spectra, and C–nuclear magnetic resonance spectra of cellulose fibers before and after the accelerated aging. The durability, properties, and mechanism of the fire-resistant “Xuan paper” based on ultralong HAP nanowires during the accelerated aging were studied. The experiments reveal the reasons for the deteriorated properties and reduced durability by aging of the traditional Xuan paper based on cellulose fibers, and the mechanism for the super-durability and excellent performances of the fire-resistant “Xuan paper” based on ultralong HAP nanowires during the accelerated aging process.
Raman and Photoluminescence Studies of Quasiparticles in van der Waals Materials
Authors: AL-Makeen Mansour M. , Biack Mario H. , Guo Xiao , Xie Haipeng , Huang Han
Published: January 2025

Two-dimensional (2D) layered materials have received much attention due to the unique properties stemming from their van der Waals (vdW) interactions, quantum confinement, and many-body interactions of quasi-particles, which drive their exotic optical and electronic properties, making them critical in many applications. Here, we review our past years’ findings, focusing on many-body interactions in 2D layered materials, including phonon anharmonicity, electron–phonon coupling (e-ph), exciton dynamics, and phonon anisotropy based on temperature (polarization)-dependent Raman spectroscopy and Photoluminescence (PL). Our review sheds light on the role of quasi-particles in tuning the material properties, which could help optimize 2D materials for future applications in electronic and optoelectronic devices.