bluuebunny/crossref_metadata_2025 huggingface dataset:
Blockchain-Based Frameworks for Food Traceability: A Systematic Review
Authors: Ellahi Rizwan Matloob , Wood Lincoln C. , Bekhit Alaa El-Din Ahmed
Published: August 2023

With the rise of globalization and technological competition, the food supply chain has grown more complex due to the multiple players and factors involved in the chain. Traditional systems fail to offer effective and reliable traceability solutions considering the increasing requirement for accountability and transparency in the food supply chain. Blockchain technology has been claimed to offer the food industry a transformative future. The inherent features of blockchain, including immutability and transparency, create a dependable and secure system for tracking food products across the whole supply chain, ensuring total control over their traceability from the origin to the final consumer. This research offers a comprehensive overview of multiple models to understand how the integration of blockchain and other digital technologies has transformed the food supply chain. This comprehensive systematic review of blockchain-based food-supply-chain frameworks aimed to uncover the capability of blockchain technology to revolutionize the industry and examined the current landscape of blockchain-based food traceability solutions to identify areas for improvement. Furthermore, the research investigates recent advancements and investigates how blockchain aligns with other emerging technologies of Industry 4.0 and Web 3.0. Blockchain technology plays an important role in improving food traceability and supply-chain operations. Potential synergies between blockchain and other emerging technologies of Industry 4.0 and Web 3.0 are digitizing food supply chains, which results in better management, automation, efficiencies, sustainability, verifiability, auditability, accountability, traceability, transparency, tracking, monitoring, response times and provenance across food supply chains.
Next-Generation Sequencing for Screening Analysis of Cystic Fibrosis: Spectrum and Novel Variants in a South–Central Italian Cohort
Authors: De Paolis Elisa , Tilocca Bruno , Lombardi Carla , De Bonis Maria , Concolino Paola , Onori Maria Elisabetta , Ricciardi Tenore Claudio , Perrucci Alessia , Roncada Paola , Capoluongo Ettore , Urbani Andrea , Minucci Angelo , Santonocito Concetta
Published: August 2023

The incidence of cystic fibrosis (CF) and the spectrum of cystic fibrosis transmembrane conductance regulator (CFTR) gene variants differ among geographic regions. Differences in CF carrier distribution are also reported among Italian regions. We described the spectrum of the CFTR variants observed in a large group of subjects belonging from central–southern Italy. We also provide a predictive evaluation of the novel variants identified. CFTR screening was performed in a south–central Italian cohort of 770 subjects. We adopted a next-generation sequencing (NGS) approach using the Devyser CFTR NGS kit on the Illumina MiSeq System coupled with Amplicon Suite data analysis. Bioinformatics evaluation of the impact of novel variants was described. Overall, the presence of at least one alternative allele in the CFTR gene was recorded for 23% of the subjects, with a carrier frequency of CF pathogenic variants of 1:12. The largest sub-group corresponded to the heterozygous carriers of a variant with a conflicting interpretation of pathogenicity. The common CFTR p.(Phe508del) pathogenic variants were identified in 37% of mutated subjects. Bioinformatics prediction supported a potential damaging effect for the three novel CFTR variants identified: p.(Leu1187Phe), p.(Pro22Thr), and c.744-3C > G. NGS applied to CF screening had the benefit of: effectively identifying asymptomatic carriers. It lies in a wide overview of CFTR variants and gives a comprehensive picture of the carrier prevalence. The identification of a high number of unclassified variants may represent a challenge whilst at the same time being of interest and relevance for clinicians.
The Impact of Weir Construction in Korea’s Nakdong River on the Population Genetic Variability of the Endangered Fish Species, Rapid Small Gudgeon (Microphysogobio rapidus)
Authors: Hong Yang-Ki , Kim Kang-Rae , Kim Keun-Sik , Bang In-Chul
Published: August 2023

Microphysogobio rapidus, an endemic cyprinid fish species found exclusively in Korea, has been identified in only two tributaries of the Nakdong River. The species predominantly occupies the near-gravel bottom waters within shallow sections of the middle and lower reaches of the river, characterized by swift currents. M. rapidus is currently recognized as a critically endangered species due to its distinct habitat preference, as well as the negative impacts of stream dam development and water environment pollution. In this study, we used 10 microsatellite markers to examine the genetic diversity of M. rapidus in the upper Nam (UN), lower Nam (LN), and Deokcheon Rivers (DC) in Korea, with a specific focus on assessment of the impact of dam development. Fish sampled from the UN and LN showed a greater average number of alleles and allelic richness (A = 18.3–18.4, AR = 13.8) compared to those from DC (A = 11.8, AR = 11.5). The observed heterozygosity among the fish examined ranged from HO = 0.748 (LN) to 0.766 (DC). All three fish groups exhibited a significant departure from Hardy–Weinberg equilibrium (HWE) (p < 0.05). Despite having the largest effective population size (Ne = 175 and 157, respectively), the fish sampled from UN and LN showed the highest inbreeding coefficients (FIS = 0.056–0.053, respectively), which were highly significant (p < 0.01). In contrast, the fish sampled from DC exhibited the smallest effective population size (Ne = 61) and showed an inbreeding coefficient close to zero (p > 0.05). BOTTLENECK analysis and estimated M-ratio values (0.341–0.372) revealed indications of past population size reduction in all fish groups examined. No significant genetic differentiation (FST < 0.05) was detected using the DAPC, STRUCTURE, and AMOVA among the fish studied. However, pairwise comparisons of FST between fish sampled from the Nam and Deokcheon Rivers revealed significant values (p < 0.001) ranging from 0.013 to 0.014. In addition, the closest genetic distance (0.026) was observed between UN and LN, while the greatest distance (0.087) was found between UN and DC. Analysis of gene flow rates among the fish examined indicated asymmetrical gene exchange within the Nam River, which was 31.51% in the downstream direction (from UN to LN), with a minimal gene flow rate (0.41%) in the upstream (from LN to UN) direction. The opposite trend was recorded between DC and LN, with a higher gene flow rate (29.74%) in the upstream direction compared to the downstream direction (0.12%). Our study highlighted the importance of implementing long-term conservation efforts focused on maintaining river integrity by removing water barriers such as weirs that impede fish migration and implementing active protection measures, such as aquaculture breeding and reasonable stocking practices, to preserve M. rapidus in the study area.
A Comprehensive Analysis of the Fowleria variegata (Valenciennes, 1832) Mitochondrial Genome and Its Phylogenetic Implications within the Family Apogonidae
Authors: Wang Jiaqiao , He Weiyi , Huang Hao , Ou Danyun , Wang Lei , Li Jun , Li Weiwen , Luo Site
Published: August 2023

Controversies surrounding the phylogenetic relationships within the family Apogonidae have persisted due to the limited molecular data, obscuring the evolution of these diverse tropical marine fishes. This study presents the first complete mitochondrial genome of Fowleria variegata, a previously unrepresented genus, using high-throughput Illumina sequencing. Through a comparative mitogenomic analysis, F. variegate was shown to exhibit a typical genome architecture and composition, including 13 protein-coding, 22 tRNA and 2 rRNA genes and a control region, consistent with studies of other Apogonidae species. Nearly all protein-coding genes started with ATG, while stop codons TAA/TAG/T were observed, along with evidence of strong functional constraints imposed via purifying selection. Phylogenetic reconstruction based on maximum likelihood and Bayesian approaches provided robust evidence that F. variegata forms a basal lineage closely related to P. trimaculatus within Apogonidae, offering novel perspectives into the molecular evolution of this family. By generating new mitogenomic resources and evolutionary insights, this study makes important headway in elucidating the phylogenetic relationships and mitogenomic characteristics of Apogonidae fishes. The findings provide critical groundwork for future investigations into the drivers of diversification, speciation patterns, and adaptive radiation underlying the extensive ecological diversity and biological success of these marine fishes using phylogenomics and population genomics approaches.
Development of Ogura CMS Fertility-Restored Interspecific Hybrids for Use in Cytoplasm Replacement of Golden-Heart Materials in Brassica rapa
Authors: Li Ze , Li Guoliang , Li Fei , Zhang Shifan , Wang Xiaowu , Wu Jian , Sun Rifei , Zhang Shujiang , Zhang Hui
Published: August 2023

Ogura cytoplasmic male sterility (CMS) is one of the important methods for hybrid seed production in cruciferous crops. The lack of a restorer of fertility gene (Rfo) in Brassica rapa L. restricts the development and utilization of its germplasm resources. In this research, Brassica napus with the Rfo gene was used to restore the fertility of Ogura CMS B. rapa with the golden heart trait. Through the distant cross of two B. rapa and four B. napus, six interspecific hybrid combinations received F1 seeds. The six combinations were different in seed receiving. By morphological observation and molecular marker-assisted selection (MAS), in F1, individuals containing the Rfo gene all appeared fertile, while those without it remained male-sterile. The pollen viability of the fertile individuals was measured, and the fertile lines of the six interspecific hybrid combinations were different (40.68–80.49%). Three individuals (containing both Rfo and GOLDEN genes) with the highest pollen vitality (≥60%) were backcrossed with fertile cytoplasmic B. rapa, resulting in a total of 800 plants. Based on the MAS, a total of 144 plants with GOLDEN but no Rfo were screened (18%). Moreover, through morphological investigation, one individual with normal cytoplasm, stable fertility but without the restoring gene Rfo, the GOLDEN gene, and morphological characteristics similar to those of B. rapa was obtained. These results increased the diversity of B. rapa germplasm and provided a new method for the utilization of CMS germplasm in Brassica crops.
Subduction as a Smoothing Machine: How Multiscale Dissipation Relates Precursor Signals to Fault Geometry
Authors: Venegas-Aravena Patricio , Cordaro Enrique G.
Published: August 2023

Understanding the process of earthquake preparation is of utmost importance in mitigating the potential damage caused by seismic events. That is why the study of seismic precursors is fundamental. However, the community studying non-seismic precursors relies on measurements, methods, and theories that lack a causal relationship with the earthquakes they claim to predict, generating skepticism among classical seismologists. Nonetheless, in recent years, a group has emerged that seeks to bridge the gap between these communities by applying fundamental laws of physics, such as the application of the second law of thermodynamics in multiscale systems. These systems, characterized by describing irreversible processes, are described by a global parameter called thermodynamic fractal dimension, denoted as D. A decrease in D indicates that the system starts seeking to release excess energy on a macroscopic scale, increasing entropy. It has been found that the decrease in D prior to major earthquakes is related to the increase in the size of microcracks and the emission of electromagnetic signals in localized zones, as well as the decrease in the ratio of large to small earthquakes known as the b-value. However, it is still necessary to elucidate how D, which is also associated with the roughness of surfaces, relates to other rupture parameters such as residual energy, magnitude, or fracture energy. Hence, this work establishes analytical relationships among them. Particularly, it is found that larger magnitude earthquakes with higher residual energy are associated with smoother faults. This indicates that the pre-seismic processes, which give rise to both seismic and non-seismic precursor signals, must also be accompanied by changes in the geometric properties of faults. Therefore, it can be concluded that all types of precursors (seismic or non-seismic), changes in fault smoothness, and the occurrence of earthquakes are different manifestations of the same multiscale dissipative system.
Review of Methods to Solve Desiccation Cracks in Clayey Soils
Authors: Levatti Hector U.
Published: August 2023

This paper reviews numerical methods used to simulate desiccation cracks in clayey soils. It examines five numerical approaches: Finite Element (FEM), Lattice Boltzmann (LBM), Discrete Element (DEM), Cellular Automaton (CAM), and Phase Field (PFM) Methods. The paper presents a simplified description of the methods, including their basic numerical formulations. Several factors such as the multiphase nature of soils, heterogeneity, nonlinearities, coupling, scales of analysis, and computational aspects are discussed. The review highlights the characteristics, strengths, and limitations of each method. FEM shows a good capacity to deal with the thermo-hydromechanical behavior of clays when drying that complement well with the ability of DEM to deal with particle interactions as well as LBM, PFM, and CAM to deal with complex crack patterns. The article concludes by reviewing the integration of multiple numerical methods to enhance the simulation of desiccation cracks in clayey soils and proposing what is the best option to continue improving the study of this problem.
Preparing Cities for Future Pandemics: Unraveling the Influence of Urban and Housing Variables on COVID-19 Incidence in Santiago de Chile
Authors: Kuschel Katherina , Carrasco Raúl , Idrovo-Aguirre Byron J. , Duran Claudia , Contreras-Reyes Javier E.
Published: August 2023

In this study, we analyzed how urban, housing, and socioeconomic variables are related to COVID-19 incidence. As such, we have analyzed these variables along with demographic, education, employment, and COVID-19 data from 32 communes in Santiago de Chile between March and August of 2020, before the release of the vaccines. The results of our Principal Component Analysis (PCA) confirmed that those communes with more economic, social, organizational, and infrastructural resources were overall less affected by COVID-19. As the dimensions affecting COVID-19 are based on structural variables, this study discusses to what extent our cities can be prepared for the next pandemic. Recommendations for local decision-makers in controlling illegal immigration and investing in housing and urban parks are drawn.
Temporomandibular Disorder Prevalence and Its Association with Lifestyle Habits in Biomedicine Students—A Cross-Sectional Study
Authors: Frka Separovic Ivan , Martinovic Dinko , Lesin Antonella , Puizina Mladinic Ema , Tokic Daria , Kumric Marko , Jurina Laura , Lupi-Ferandin Marino , Bukic Josipa , Bozic Josko
Published: August 2023

This study aimed to examine the frequency of temporomandibular disorder among biomedical students and relate its occurrence to lifestyle habits. A cross-sectional collection of data was carried out and included a total of 676 examinees through a questionnaire that had 73 questions: general information and lifestyle habits, the Fonseca Anamnestic index (FAI), the Jaw Function Limitation Scale (JFLS), and the Perceived Stress Questionnaire (PSQ). The statistical analyses between three or more groups were conducted using the one-way analysis of variance (ANOVA) with post hoc Scheffé test or Kruskal–Wallis test with post hoc Dunn’s test for quantitative variables. The comparison of qualitative variables was conducted using the Chi-square test, while the correlations were determined using Spearman’s correlation test. The analysis showed that a higher frequency of moderate or severe TMD was observed in subjects who were smokers (p < 0.001) compared to non-smokers. Subjects who consumed more coffee had moderate to severe TMD compared to subjects who consumed a lesser amount (p < 0.001). Furthermore, a positive correlation between the amount of stress and the severity of TMD was found. Our study implies that students of biomedical studies have an increased risk for TMD and that there is a link with their lifestyle habits.
The Influence of Food Regimes on Oxidative Stress: A Permutation-Based Approach Using the NPC Test
Authors: Zirilli Agata , Ruggeri Rosaria Maddalena , Barbalace Maria Cristina , Hrelia Silvana , Giovanella Luca , Campennì Alfredo , Cannavò Salvatore , Alibrandi Angela
Published: August 2023

(1) Background: This paper aims to assess the existence of significant differences between two dietary regimes (omnivorous vs. semi-vegetarian) with reference to some oxidative stress markers (SOD, GPx, TRxR, GR, AGEs, and AOPPs) using non-parametric combination methodology based on a permutation test. (2) Methods: At the endocrinology unit of Messina University Hospital, two hundred subjects were asked to fill out a questionnaire about their dietary habits. None were under any pharmacological treatment. Using the NPC test, all comparisons were performed stratifying patients according to gender, age (≤40 or >40 years), BMI (normal weight vs. overweight), physical activity (sedentary vs. active lifestyle), TSH, FT4 levels in quartiles, and diagnosis of Hashimoto’s thyroiditis. We evaluated differences in oxidative stress parameters in relation to two examined dietary regimes (omnivorous vs. semi-vegetarian). (3) Results: The antioxidant parameters GPx and TRxR were significantly lower in subjects with an omnivorous diet than in semi-vegetarians, particularly in females, both age groups, subjects with normal weight, those not affected by Hashimoto’s thyroiditis, and both the sedentary and active lifestyle groups. Finally, the AGE and AOPP markers were significantly lower in semi-vegetarians. (4) Conclusion: Thanks to the NPC methodology, we can state that dietary patterns exert a significant influence on some oxidative stress parameters.
Effect of Acupuncture on Recovery of Consciousness in Patients with Acute Traumatic Brain Injury: A Multi-Institutional Cohort Study
Authors: Lin Chun-Chieh , Chen Hsing-Yu , Tseng Chu-Yao , Yang Chien-Chung
Published: August 2023

Traumatic brain injury (TBI) causes cognitive dysfunction and long-term impairments. This study aims to examine the effectiveness of acupuncture on the recovery of consciousness in TBI patients. This is a retrospective, multi-institutional cohort study. We enrolled patients with newly diagnosed TBI from 1 January 2007 to 3 August 2021, aged 20 years and older, from the Chang Gung Research Database (CGRD). The outcome was defined based on the difference between the first and last Glasgow Coma Scale (GCS). A total of 2163 TBI patients were analyzed, and 237 (11%) received acupuncture in the treatment period. Generally, the initial GCS was lower in the acupuncture users (11 vs. 14). For the results of our study, a higher proportion of acupuncture patients achieved significant improvement (GCS differences ≥ 3) compared to non-acupuncture users (46.0% vs. 22.4%, p-value < 0.001). The acupuncture users had a 2.11 times higher chance of achieving a significant improvement when considering all assessable covariates (adjusted odds ratio (aOR) 2, 11, 95% confidence interval [CI]: 1.31–3.40; p-value = 0.002). Using 1:1 propensity score matching (PSM), the acupuncture users still had better outcomes than the non-acupuncture users (45.3% vs. 32.9%, p-value = 0.020). In conclusion, this study suggests that acupuncture treatment may be beneficial for TBI patients.
Comparability of the Effectiveness of Different Types of Exercise in the Treatment of Achilles Tendinopathy: A Systematic Review
Authors: Sivrika Aikaterini Pantelis , Papadamou Eleni , Kypraios George , Lamnisos Demetris , Georgoudis George , Stasinopoulos Dimitrios
Published: August 2023

Achilles tendinopathy (AT) is a common condition both in athletes and the general population. The purpose of this study is to highlight the most effective form of exercise in managing pain-related symptoms and functional capacity as well as in a return to life activities, ensuring the quality of life of patients with AT, and creating a protocol to be used in rehabilitation. We conducted a systematic review of the published literature in Pubmed, Scopus, Science Direct, and PEDro for Randomised Controlled Trials concerning interventions that were based exclusively on exercise and delivered in patients 18–65 years old, athletes and non-athletes. An amount of 5235 research articles generated from our search. Five met our inclusion criteria and were included in the review. Research evidence supports the effectiveness of a progressive loading eccentric exercise program based on Alfredson’s protocol, which could be modified in intensity and pace to fit the needs of each patient with AT. Future research may focus on the optimal dosage and load of exercise in eccentric training and confirm the effectiveness of other type of exercise, such as a combination of eccentric–concentric training or heavy slow resistance exercise. Pilates could be applied as an alternative, useful, and friendly tool in the rehabilitation of AT.
Professional Accountability of Caring for Patients with COVID-19: A Phenomenographic Study
Authors: Chen Li-Chin , Yeh Shu-Ling , Lee Hui-Ling , Lin Chun-Chih , Goopy Suzanne , Han Chin-Yen
Published: August 2023

The purpose of this study was to explore nurses’ care experiences for COVID-19 patients during the pandemic in Taiwan. The qualitative approach of phenomenography was used. Thirty-four nurses were recruited from two assigned hospitals in which COVID-19 patients were treated in Taiwan from July to May 2021. The method of data collection in the study involved a semi-structured interview and drawing. Interviews were audio-recorded and transcribed verbatim. Phenomenographic analysis was used to analyze the qualitative data. Four categories of description of experiences of caring for COVID-19 patients were identified: facing uncountable stresses from all sides, strict implementation of infection control interventions to provide safe care, confronting ethical dilemmas and making difficult decisions, and reflecting on the meaning of care in nursing. Professional accountability was the core theme found to represent the central meaning of nurses caring for COVID-19 patients. Nurses were under enormous stress while caring for COVID-19 patients during the pandemic and were negatively affected physically, psychologically, and socially. Professional accountability in caring for COVID-19 patients can be enhanced through adequate support from nursing managers and by in-service training designed to update knowledge and skills related to infection control intervention.
Case Report-Driven Medical Education in Rural Family Medicine Education: A Thematic Analysis
Authors: Ohta Ryuichi , Sano Chiaki
Published: August 2023

Case-based education (CBE) is a teaching method in which learners work on real-life cases to learn and apply concepts and skills they have been taught. Case report-driven medical education using the CBE framework can effectively facilitate student and resident learning, and entice them to become involved in actual clinical practice. Specific case report-driven medical education methods and learning outcomes are not clarified. This study aimed to clarify the specific learning processes and outcomes of case report-driven medical education in rural community-based medical education. Using a qualitative design based on a thematic analysis approach, data were collected through semi-structured interviews. The study participants were medical students and residents in training at a rural Japanese community hospital. Fifty-one case reports were completed and published in Cureus from April 2021 to March 2023. Participants learned about various difficulties related to volatility, uncertainty, complexity, and ambiguity (VUCA) in the medical care of various older patients, which increased their interest in family medicine. They appreciated the importance of case reports in academic careers and how their responsibilities as researchers increase with collaboration. Case report-driven medical education in community hospitals can drive medical students’ and junior residents’ learning regarding family medicine in the VUCA world.
Missed Essex-Lopresti Injury—Development of a Combined Proximal and Distal Radio-Ulnar Joint Prosthesis as a Treatment Option and Proof of Concept
Authors: Oeckenpöhler Simon , Langer Martin Franz , Riesenbeck Oliver
Published: August 2023

Essex-Lopresti injuries are characterized by injuries to the proximal radio-ulnar joint, the distal radio-ulnar joint, and the interosseous membrane. This can lead to osteoarthritis, impaction syndrome, or instability. If all three structures are injured and lead to instability, the situation is almost unmanageable and many times ends in a one-bone forearm. In this article, we demonstrate a new way to reconstruct the proximal and distal radio-ulnar joint with two patient-specific coupled prostheses. These have been developed with the biomechanical conditions of the forearm in mind, where there are very large forces between the bones. As a result, we are able to present a patient previously severely restricted in the use of his hand and arm via a splint that compressed the forearm, who is now able to perform everyday activities and even light sports, such as badminton, without pain.
Path of Knowledge for the Assessment of Structural Safety of the Pisan Tower of the Royal Palace of Palermo in Italy
Authors: La Mendola Lidia , Accardi Matteo , Agnello Fabrizio , Monaco Alessia
Published: August 2023

This paper presents the path of knowledge developed for assessing the structural safety of the Norman-age Pisan Tower, which is mostly incorporated into the Royal Palace in Palermo, Italy. Historical, geomatic, and mechanical investigations were conducted and the most relevant results are herein collected and presented. The research path was addressed to specific tasks: identification of the building, geometric surveys, recognition of the sequence of phases of building transformation, detection of the components of the load-bearing structure, structural diagnostic surveys, and investigation of the subsoil and foundations. The explicit vulnerabilities found were mostly confined to the Piazzi library floor, while implicit vulnerabilities were identified in the presence of false walls and in high loads and fillings on the vaults of the last levels. The results of the analyses allowed the individuation of the confidence factors to use in structural analysis models aimed at the assessment of the seismic safety of the building.
Functional Verification of the Four Splice Variants from Ajania purpurea NST1 in Transgenic Tobacco
Authors: Wang Hai , Hao Xueying , Zhang Wenxin , Guo Yuning , Zhao Xiang , Li Yanxi , He Wenting , Cai Shiyi , Song Xuebin
Published: August 2023

Ajania purpurea is a small semi-shrub in the Asteraceae family. Its corolla is purplish red from the middle to the top, and its leaves and flowers are all fragrant. It can be introduced and cultivated as ornamental plants. In order to survive adversity, plants actively regulate the expression of stress response genes and transcripts. Alternative splicing is a common phenomenon and an important regulation mode of eukaryotic gene transcription, which plays an important role in various biological processes. In this study, four splice variants of the NST1 gene were identified from A. purpurea, and the molecular mechanism of NST1 alternative splice variants involved in abiotic stress was explored through bioinformatics, transgenics and paraffin sectionalization. The analysis of amino acid sequences showed that ApNST1.1 had alternative 5′splicing, ApNST1.2 had alternative 3′splicing and ApNST1 had the two splicing types. The main conclusions from studying transgenic tobacco seedlings and adult seedlings under abiotic stress were as follows: ApNST1, ApNST1.1 and ApNST1.3 showed salt tolerance at seedling stage, especially ApNST1.3. At the mature seedling stage, the stem height of ApNST1.1 increased significantly, and ApNST1.1 showed obvious salt tolerance, while ApNST1.2 showed obvious cold resistance. Compared to Super35S::GFP, the xylem of ApNST1 thickened by 94 μm, and the cell wall thickened by 0.215 μm. These results are of great significance to the breeding and application of ApNST1 to select splice variants with more resistance to abiotic stress, and to future study in this area. At the same time, they provide a new direction for A. purpurea breeding, and increase the possibility of garden applications.
Using Ensembles of Machine Learning Techniques to Predict Reference Evapotranspiration (ET0) Using Limited Meteorological Data
Authors: Salahudin Hamza , Shoaib Muhammad , Albano Raffaele , Inam Baig Muhammad Azhar , Hammad Muhammad , Raza Ali , Akhtar Alamgir , Ali Muhammad Usman
Published: August 2023

To maximize crop production, reference evapotranspiration (ET0) measurement is crucial for managing water resources and planning crop water needs. The FAO-PM56 method is recommended globally for estimating ET0 and evaluating alternative methods due to its extensive theoretical foundation. Numerous meteorological parameters, needed for ET0 estimation, are difficult to obtain in developing countries. Therefore, alternative ways to estimate ET0 using fewer climatic data are of critical importance. To estimate ET0 with alternative methods, difference climatic parameters of temperatures, relative humidity (maximum and minimum), sunshine hours, and wind speed for a period of 20 years from 1996 to 2015 were used in the study. The data were recorded by 11 meteorological observatories situated in various climatic regions of Pakistan. The significance of the climatic parameters used was evaluated using sensitivity analysis. The machine learning techniques of single decision tree (SDT), tree boost (TB) and decision tree forest (DTF) were used to perform sensitivity analysis. The outcomes indicated that DTF-based models estimated ET0 with higher accuracy and fewer climatic variables as compared to other ML techniques used in the study. The DTF technique, with Model 15 as input, outperformed other techniques for the most part of the performance metrics (i.e., NSE = 0.93, R2 = 0.96 and RMSE = 0.48 mm/month). The results indicated that the DTF with fewer climatic variables of mean relative humidity, wind speed and minimum temperature could estimate ET0 accurately and outperformed other ML techniques. Additionally, a non-linear ensemble (NLE) of ML techniques was further used to estimate ET0 using the best input combination (i.e., Model 15). It was seen that the applied non-linear ensemble (NLE) approach enhanced modelling accuracy as compared to a stand-alone application of ML techniques (R2 Multan = 0.97, R2 Skardu = 0.99, R2 ISB = 0.98, R2 Bahawalpur = 0.98 etc.). The study results affirmed the use of an ensemble model for ET0 estimation and suggest applying it in other parts of the world to validate model performance.
Health-Related Quality of Life, Stress, Caregiving Burden and Special Needs of Parents Caring for a Short-Statured Child—Review and Recommendations for Future Research
Authors: Lackner Lea , Quitmann Julia , Kristensen Kaja , Witt Stefanie
Published: August 2023

Children with short stature can experience a range of burdens due to their chronic condition. However, little is known about parents’ experiences dealing with their child’s short stature and the potential caregiving burdens and concerns they may face. We aim to review the literature on health-related quality of life (HRQOL), caregiving burden, and special needs among parents caring for a child with isolated growth hormone deficiency (IGHD) or idiopathic short stature (ISS). Using pre-defined inclusion and exclusion criteria, we systematically searched for literature using PubMed and Web of Science from its inception to December 2022. We identified 15 articles assessing HRQOL, special needs, or caregiving burdens in parents of IGHD/ISS children. The main problems included concerns about the future, organizational issues, side effects from growth hormone treatment, and social stigmatization. Furthermore, two studies assessed parents’ special needs to cope with caregiving stress, mainly the dialogue between them and their families or parent support groups. This review outlines parental burdens, needs, and resources when caring for an IGHD/ISS child. Furthermore, it provides information about previously used measures appraising parents’ special needs and underlines the need for disease-specific measurements.
Characteristics of Commercial and Raw Pellets Available on the Italian Market: Study of Organic and Inorganic Fraction and Related Chemometric Approach
Authors: Pandolfi Pietro , Notardonato Ivan , Passarella Sergio , Sammartino Maria Pia , Visco Giovanni , Ceci Paolo , De Giorgi Loretta , Stillittano Virgilio , Monci Domenico , Avino Pasquale
Published: August 2023

Air pollution and the increasing production of greenhouse gases has prompted greater use of renewable energy sources; the EU has set a target that the use of green energy should be at 32 percent by 2030. With this in mind, in the last 10 years, the demand for pellets in Italy has more than doubled, making Italy the second largest consumer in Europe. The quality of the pellets burned in stoves is crucial to indoor and outdoor pollution. Among other parameters, moisture and ash are used to classify pellets according to EN ISO 17225:2014. This work involved the analysis of the organic and inorganic fraction of both some finished products on the Italian market and some raw materials (e.g., wood chips) sampled according to the technical standard EN 14778:2011. The analytical results showed the presence of some substances potentially harmful to human health such as formaldehyde, acetone, toluene and styrene for the organic fraction and nickel, lead and vanadium for the inorganic fraction. The chemometric approach showed that it is the inorganic fraction which is most responsible for the diversification of the samples under study. The detection of some substances may be a warning bell about the impact of such materials, both for the environment and for human health.
Attachment Stability and Longitudinal Prediction of Psychotic-like Symptoms in Community Adolescents over Four Months of COVID-19 Pandemic
Authors: Pace Cecilia Serena , Muzi Stefania , Morganti Wanda , Steele Howard
Published: August 2023

Background: The Friends and Family Interview (FFI) is assumed to be a valid method to study attachment stability and attachment-related psychopathological processes in adolescence, but no studies have yet tested the test–retest reliability of this interview or the longitudinal association of attachment patterns in response to the FFI from adolescents with symptoms such as psychotic-like experiences (e.g., hallucinations, bizarre behavior, dissociation, self-harm) that are known to have increased during the COVID-19 pandemic. Methods: This study involved 102 community adolescents (M = 14.64, SD = 1.63, 46% males) assessed twice: during a severe COVID-19-related lockdown (in Italy) (T1) and four months later (T2). Measures were the FFI (assessing attachment patterns: secure-autonomous, insecure-dismissing, insecure-preoccupied, and insecure-disorganized) and the thought problems scale of the Youth Self-Report to assess psychotic-like symptoms. Results: revealed high stability of four-way attachment classifications over four months (93.5%), with a modest yet significant link between higher disorganization at T1 and higher scores of thought problems at T2, p = 0.010. Conclusions: The FFI shows high test–retest reliability and can be a valid, age-adapted option to assess adolescents’ attachment. Attachment disorganization should be further investigated as possibly related to psychotic-like experiences in community adolescents.
Bibliometric Review of Blended Finance and Partial Risk Guarantee: Establishing Needs and Advantages
Authors: Sharma Kamakshi , Mahna Tusharika , Jain Sonali , Dhir Sanjay , Rao Neeta , Biyani Achin , Sikka Himanshu , Yadav Rishit , Dua Sidharth , Gupta Archish
Published: August 2023

A partial risk guarantee (PRG) is one of the critical instruments in the blended finance approach that provides partial assurance to the risk investor to lend leveraged capital to the borrower. Under the PRG scheme, philanthropic capital is employed as a risk guarantee to create financial and economic additionality through the multiplier effect. This study examines the current trends in PRG and blended finance ecosystem research. This study also aims to identify future research areas to work upon. The bibliometric analysis highlights the need and advantages of blended finance and PRG. The study highlights themes, such as climate finance, SDGs, impact investments, and blended finance/PRGs, from the literature on blended finance. This study illustrates the impact for researchers and managers regarding the future direction to undertake and the domains where PRG can work wonders. The research allows for a comprehensive view of the leading trends, such as utilising blended finance tools such as PRG in funding the work in climate financing, SDGs, water, sanitation, and impact investment. This is perhaps the first study to conduct a bibliometric analysis of the developing area of blended finance partial risk guarantee literature to highlight its importance and advantages.
A Method for Intelligent Road Network Selection Based on Graph Neural Network
Authors: Guo Xuan , Liu Junnan , Wu Fang , Qian Haizhong
Published: August 2023

As an essential role in cartographic generalization, road network selection produces basic geographic information across map scales. However, the previous selection methods could not simultaneously consider both attribute characteristics and spatial structure. In light of this, an intelligent road network selection method based on a graph neural network (GNN) is proposed in this paper. Firstly, the selection case is designed to construct a sample library. Secondly, some neighbor sampling and aggregation rules are developed to update road features. Then, a GNN-based selection model is designed to calculate classification labels, thus completing road network selection. Finally, a few comparative analyses with different selection methods are conducted, verifying that most of the accuracy values of the GNN model are stable over 90%. The experiments indicate that this method could aggregate stroke nodes and their neighbors together to synchronously preserve semantic, geometric, and topological features of road strokes, and the selection result is closer to the reference map. Therefore, this paper could bridge the distance between deep learning and cartographic generalization, thus facilitating a more intelligent road network selection method.
A Novel Mechanically Robust and Biodegradable Egg White Hydrogel Membrane by Combined Unidirectional Nanopore Dehydration and Annealing
Authors: Dong Xuan , Zhang Yu-Qing
Published: August 2023

A homogeneous egg white obtained by high-speed shearing and centrifugation was dehydrated into a fragile and water-soluble egg white glass (EWG) by unidirectional nanopore dehydration (UND). After EWG annealing, it can become an egg white hydrogel membrane (EWHM) that is water-insoluble, flexible, biocompatible, and mechanically robust. Its tensile strength, elongation at break, and the swelling ratio are about 5.84 MPa, 50–110%, and 60–130%, respectively. Protein structure analysis showed that UND caused the rearrangement of the protein molecules to form EWG with random coil and α-helix structures. The thermal decomposition temperature of the EWG was 309.25 °C. After EWG annealing at over 100 or 110 °C for 1.0 h or 45 min, the porous network EWHM was mainly composed of β-sheet structures, and the thermal decomposition temperature increased to 317.25–318.43 °C. Their 12-day residues in five proteases ranged from 1% to 99%, and the order was pepsin > neutral protease > papain > trypsin > alkaline protease. Mouse fibroblast L929 cells can adhere, grow, and proliferate well on these EWHMs. Therefore, the combined technology of UND and annealing for green and novel processing of EWHM has potential applications in the field of biomimetic and biomedical materials.
The Role of PIK3R1 in Metabolic Function and Insulin Sensitivity
Authors: Tsay Ariel , Wang Jen-Chywan
Published: August 2023

PIK3R1 (also known as p85α) is a regulatory subunit of phosphoinositide 3-kinases (PI3Ks). PI3K, a heterodimer of a regulatory subunit and a catalytic subunit, phosphorylates phosphatidylinositol into secondary signaling molecules involved in regulating metabolic homeostasis. PI3K converts phosphatidylinositol 4,5-bisphosphate (PIP2) to phosphatidylinositol 3,4,5-triphosphate (PIP3), which recruits protein kinase AKT to the inner leaflet of the cell membrane to be activated and to participate in various metabolic functions. PIK3R1 stabilizes and inhibits p110 catalytic activity and serves as an adaptor to interact with insulin receptor substrate (IRS) proteins and growth factor receptors. Thus, mutations in PIK3R1 or altered expression of PIK3R1 could modulate the activity of PI3K and result in significant metabolic outcomes. Interestingly, recent studies also found PI3K-independent functions of PIK3R1. Overall, in this article, we will provide an updated review of the metabolic functions of PIK3R1 that includes studies of PIK3R1 in various metabolic tissues using animal models, the mechanisms modulating PIK3R1 activity, and studies on the mutations of human PIK3R1 gene.
Non-Thermal Plasma Application in Medicine—Focus on Reactive Species Involvement
Authors: Moszczyńska Julia , Roszek Katarzyna , Wiśniewski Marek
Published: August 2023

Non-thermal plasma (NTP) application in medicine is a dynamically developing interdisciplinary field. Despite the fact that basics of the plasma phenomenon have been known since the 19th century, growing scientific attention has been paid in recent years to the use of plasma in medicine. Three most important plasma-based effects are pivotal for medical applications: (i) inactivation of a broad spectrum of microorganisms, (ii) stimulation of cell proliferation and angiogenesis with lower plasma treatment intensity, and (iii) inactivation of cells by initialization of cell death with higher plasma intensity. In this review, we explain the underlying chemical processes and reactive species involvement during NTP in human (or animal) tissues, as well as in bacteria inactivation, which leads to sterilization and indirectly supports wound healing. In addition, plasma-mediated modifications of medical surfaces, such as surgical instruments or implants, are described. This review focuses on the existing knowledge on NTP-based in vitro and in vivo studies and highlights potential opportunities for the development of novel therapeutic methods. A full understanding of the NTP mechanisms of action is urgently needed for the further development of modern plasma-based medicine.
Simultaneous Comparison of Aqueous Humor and Serum Metabolic Profiles of Diabetic and Nondiabetic Patients Undergoing Cataract Surgery—A Targeted and Quantitative Metabolomics Study
Authors: Grochowski Emil Tomasz , Pietrowska Karolina , Godlewski Adrian , Gosk Wioleta , Buczynska Angelika , Wojnar Malgorzata , Konopinska Joanna , Kretowski Adam , Ciborowski Michal , Dmuchowska Diana Anna
Published: August 2023

The aim of this study was to compare the aqueous humor (AH) and serum concentrations of metabolites in diabetic (n = 36) and nondiabetic (n = 36) senior adults undergoing cataract surgery. Blood samples were collected before surgery and AH during surgery. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS)-based targeted metabolomic and lipidomic analyses of samples were performed using the AbsoluteIDQ® p180 kit. Out of 188 metabolites targeted by the kit, 41 and 133 were detected in >80% of AH and serum samples, respectively. Statistical analysis performed to indicate metabolites differentiating diabetic and nondiabetic patients showed 8 and 20 significant metabolites in AH and serum, respectively. Pathway analysis performed for significant metabolites revealed that galactose metabolism is mostly affected in the AH, while arginine biosynthesis is mostly affected in the serum. Among metabolites that differentiate diabetic and nondiabetic patients, arginine was the only metabolite common to both serum and AH samples, as well as the only one with a decreased concentration in both body fluids of diabetic patients. Concentrations of the rest were elevated in AH and lowered in serum. This may suggest different mechanisms of diabetes-related dysregulation of the local metabolism in the eye in comparison to systemic changes observed in the blood.
Role of Na+/Ca2+ Exchanger (NCX) in Glioblastoma Cell Migration (In Vitro)
Authors: Brandalise Federico , Ramieri Martino , Pastorelli Emanuela , Priori Erica Cecilia , Ratto Daniela , Venuti Maria Teresa , Roda Elisa , Talpo Francesca , Rossi Paola
Published: August 2023

Glioblastoma (GBM) is the most malignant form of primary brain tumor. It is characterized by the presence of highly invasive cancer cells infiltrating the brain by hijacking neuronal mechanisms and interacting with non-neuronal cell types, such as astrocytes and endothelial cells. To enter the interstitial space of the brain parenchyma, GBM cells significantly shrink their volume and extend the invadopodia and lamellipodia by modulating their membrane conductance repertoire. However, the changes in the compartment-specific ionic dynamics involved in this process are still not fully understood. Here, using noninvasive perforated patch-clamp and live imaging approaches on various GBM cell lines during a wound-healing assay, we demonstrate that the sodium-calcium exchanger (NCX) is highly expressed in the lamellipodia compartment, is functionally active during GBM cell migration, and correlates with the overexpression of large conductance K+ channel (BK) potassium channels. Furthermore, a NCX blockade impairs lamellipodia formation and maintenance, as well as GBM cell migration. In conclusion, the functional expression of the NCX in the lamellipodia of GBM cells at the migrating front is a conditio sine qua non for the invasion strategy of these malignant cells and thus represents a potential target for brain tumor treatment.