bluuebunny/crossref_metadata_2025 huggingface dataset:
A single n-type semiconducting polymer-based photo-electrochemical transistor
Authors: Druet Victor , Ohayon David , Petoukhoff Christopher E. , Zhong Yizhou , Alshehri Nisreen , Koklu Anil , Nayak Prem D. , Salvigni Luca , Almulla Latifah , Surgailis Jokubas , Griggs Sophie , McCulloch Iain , Laquai Frédéric , Inal Sahika
Published: September 2023

Conjugated polymer films, which can conduct both ionic and electronic charges, are central to building soft electronic sensors and actuators. Despite the possible interplay between light absorption and the mixed conductivity of these materials in aqueous biological media, no single polymer film has been utilized to create a solar-switchable organic bioelectronic circuit that relies on a fully reversible and redox reaction-free potentiometric photodetection and current modulation. Here we demonstrate that the absorption of light by an electron and cation-transporting polymer film reversibly modulates its electrochemical potential and conductivity in an aqueous electrolyte, which is harnessed to design an n-type photo-electrochemical transistor (n-OPECT). By controlling the intensity of light incident on the n-type polymeric gate electrode, we generate transistor output characteristics that mimic the modulation of the polymeric channel current achieved through gate voltage control. The micron-scale n-OPECT exhibits a high signal-to-noise ratio and an excellent sensitivity to low light intensities. We demonstrate three direct applications of the n-OPECT, i.e., a photoplethysmogram recorder, a light-controlled inverter circuit, and a light-gated artificial synapse, underscoring the suitability of this platform for a myriad of biomedical applications that involve light intensity changes.
Ferromagnetism and ferroelectricity in a superlattice of antiferromagnetic perovskite oxides without ferroelectric polarization
Authors: Rout Paresh C. , Ray Avijeet , Schwingenschlögl Udo
Published: September 2023

We study the structural, electronic, and magnetic properties of the SrCrO3/YCrO3 superlattice and their dependence on epitaxial strain. We discover that the superlattice adopts A-type antiferromagnetic (A-AFM) ordering in contrast to its constituents (SrCrO3: C-AFM; YCrO3: G-AFM) and retains it under compressive strain while becoming ferromagnetic (5 μB per formula unit) at +1% strain. The obtained ferroelectric polarization is significantly higher than that of the R2NiMnO6/La2NiMnO6 (R = Ce to Er) series of superlattices [Nat. Commun. 5, 4021 (2014)] due to a large difference between the antipolar displacements of the Sr and Y cations. The superlattice is a hybrid-improper multiferroic material with a spontaneous ferroelectric polarization (13.5 μC/cm2) approaching that of bulk BaTiO3 (19 μC/cm2). The combination of ferromagnetism with ferroelectricity enables multistate memory applications. In addition, the charge-order-driven p-type semiconducting state of the ferromagnetic phase (despite the metallic nature of SrCrO3) is a rare property and interesting for spintronics. Monte Carlo simulations demonstrate a magnetic critical temperature of 90 K for the A-AFM phase without strain and of 115 K for the ferromagnetic phase at +5% strain, for example.
Monthly quasi-periodic eruptions from repeated stellar disruption by a massive black hole
Authors: Evans P. A. , Nixon C. J. , Campana S. , Charalampopoulos P. , Perley D. A. , Breeveld A. A. , Page K. L. , Oates S. R. , Eyles-Ferris R. A. J. , Malesani D. B. , Izzo L. , Goad M. R. , O’Brien P. T. , Osborne J. P. , Sbarufatti B.
Published: September 2023

In recent years, searches of archival X-ray data have revealed galaxies exhibiting nuclear quasi-periodic eruptions with periods of several hours. These are reminiscent of the tidal disruption of a star by a supermassive black hole. The repeated, partial stripping of a white dwarf in an eccentric orbit around an ~105 M⊙ black hole provides an attractive model. A separate class of periodic nuclear transients, with much longer timescales, have recently been discovered optically and may arise from the partial stripping of a main-sequence star by an ~107 M⊙ black hole. No clear connection between these classes has been made. We present the discovery of an X-ray nuclear transient that shows quasi-periodic outbursts with a period of weeks. We discuss possible origins for the emission and propose that this system bridges the two existing classes outlined above. This discovery was made possible by the rapid identification, dissemination and follow-up of an X-ray transient found by the new live Swift-XRT transient detector, demonstrating the importance of low-latency, sensitive searches for X-ray transients.
Applying FAIR4RS principles to develop an integrated modeling environment for the magnetic confinement fusion
Authors: Liu Xiaojuan , Yu Zhi , Xiang Nong
Published: September 2023

Over the decades, the integrated modeling (IM) environment for magnetically confined fusion has evolved from a single, isolated, proprietary numerical computing software to an open, flexible platform emphasizing sharing, communication, and workflow. This development direction is consistent with the FAIR4RS principles put forward by the scientific community in recent years. In this article, we describe how the FAIR4RS principles were put into practice during the development of the IM management tool FyDev for the Experimental Advanced Superconducting Tokamak (EAST). FyDev integrates the process of building, deploying, and invoking research software, automating the entire process. FyDev can also assign a unique ID for each software, convert the software ID to a Python module, and encapsulate a package management tool to enhance the software building process, ensuring consistency throughout the entire phase of the research software find, access, use, and invocation in a uniform contextual environment.
Quantifying the behavioural consequences of shark ecotourism
Authors: Gayford Joel H. , Pearse William D. , De La Parra Venegas Rafael , Whitehead Darren A.
Published: September 2023

Shark populations globally are facing catastrophic declines. Ecotourism has been posited as a potential solution to many of the issues facing shark conservation, yet increasingly studies suggest that such activity may negatively influence aspects of shark ecology and so further pressure declining populations. Here we combine UAV videography with deep learning algorithms, multivariate statistics and hidden Markov models (HMM) to quantitatively investigate the behavioural consequences of ecotourism in the whale shark (Rhincodon typus). We find that ecotourism increases the probability of sharks being in a disturbed behavioural state, likely increasing energetic expenditure and potentially leading to downstream ecological effects. These results are only recovered when fitting models that account for individual variation in behavioural responses and past behavioural history. Our results demonstrate that behavioural responses to ecotourism are context dependent, as the initial behavioural state is important in determining responses to human activity. We argue that models incorporating individuality and context-dependence should, wherever possible, be incorporated into future studies investigating the ecological impacts of shark ecotourism, which are only likely to increase in importance given the expansion of the industry and the dire conservation status of many shark species.
Quantifying systemic molecular networks affected during high altitude de-acclimatization
Authors: Paul Subhojit , Jain Shikha , Gangwar Anamika , Mohanty Swaraj , Khan Nilofar , Ahmad Yasmin
Published: September 2023

High altitude acclimatization and disease have been the centerpiece of investigations concerning human health at high altitude. Almost all investigations have focused on either understanding and ameliorating high altitude disease or finding better methods of acclimatization/training at high altitude. The aspect of altitude de-induction/de-acclimatization has remained clouded despite the fact that it was documented since the first decade of twentieth century. A few recent studies, particularly in China, have stated unanimously that high altitude de-acclimatization involved multiple observable clinical symptoms ranging from headache to abdominal distention. These symptoms have been collectively referred to as “high altitude de-acclimatization syndrome” (HADAS). However, computational omics and network biology centric investigations concerning HADAS are nascent. In this study, we focus on the quantitative proteo-informatics, especially network biology, of human plasma proteome in individuals who successfully descended from high altitude areas after a stay of 120 days. In brief, the protein list was uploaded into STRING and IPA to compute z-score based cut-offs which were used to analyze the directionality and significance of various identified protein networks as well as the proteins within them. Relevant upstream regulators extracted using computational strategies were also validated. Time-points till the 180th day of de-induction have been investigated to comparatively assess the changes in the plasma proteome and protein pathways of such individuals since the 7th day of arrival at altitude. Our investigation revealed extensive effects of de-induction on lipid metabolism, inflammation and innate immune system as well as coagulation system. This novel study provides a conceptual framework for formulating therapeutic strategies to ease the symptoms of HADAS during de-acclimatization. Such strategies should focus on normalization of lipid metabolism, inflammatory signaling and coagulation systems.
Assessment of the superior photocatalytic properties of Sn2+-containing SnO2 microrods on the photodegradation of methyl orange
Authors: Jorgetto Alexandre de Oliveira , Boldrin Zanoni Maria Valnice , Orlandi Marcelo Ornaghi
Published: September 2023

A microporous Sn2+-containing SnO2 material presenting microrod morphology and a surface area of 93.0 m2 g–1 was synthesized via a simple hydrothermal route. Sn2+ ions were detected in the interior of the material (15.8 at.%) after the corrosion of a sample through sputtering. The material’s optical properties have demonstrated the absorption of a considerable fraction of visible light up to wavelengths of 671 nm, due to the presence of Sn2+ states in the material’s band structure. The analysis of the internal crystalline structure of a single microrod was carried out with the aid of a focused ion beam microscope and indicated that the material is mesocrystalline down to nanoscale level. It was proposed that the Sn2+ ions occupy intergranular sites in the highly defective crystalline structure of the material and that Sn2+ states, as well as its relatively large surface area, are responsible for the material’s superior photoactivity. The synthesized material was tested as a photocatalyst to decompose hazardous contaminants in water. The photocatalytic performance of the material was much higher than those of commercial TiO2 and SnO2 materials, decomposing nearly all methyl orange (an azo-dye model) content in water (10 mg L–1) in 6 min under UV irradiation for a photocatalyst dose of 5.33 g L–1. The photodegradation of methyl orange was also verified under visible light.
Effects of localization of uterine adenomyosis on clinical features and pregnancy outcome
Authors: Shi Jinghua , Wu Yushi , Li Xiaoyan , Gu Zhiyue , Zhang Chenyu , Yan Hailan , Dai Yi , Leng Jinhua
Published: September 2023

The purpose of this study was to implore the association among clinical features, long-term fertility outcomes and the anatomical location of adenomyosis identified by ultrasound. We collected data of non-pregnant patients between 20 and 40 years old who had undergone surgical exploration for benign gynecological conditions at our institution between January 2010 and December 2017. A total of 158 women met the inclusion criteria and were allocated into three groups according to the ultrasound-determined adenomyosis anatomical location: anterior (Group A), posterior (Group B), both posterior and anterior (Group C). 44.3% (70/158) adenomyosis was located at the posterior side. History of miscarriage and parity were significantly higher in Group C (p = 0.036 and 0.001 respectively). Group C also had a higher concurrence rate of ovarian endometrioma (OEM) (80.4%, p = 0.002), pelvic adhesion (80.4%, P = 0.003) and the revised American Fertility Society (rAFS) Score (median64, range2-100, P < 0.001), while a significantly lower rate of concurrent peritoneal endometriosis (P = 0.01). Group B showed a relative higher rate of coexistent heavy menstrual bleeding (28.6%, p = 0.04) and oviduct obstruction (24.3%, P = 0.038). Group A had a higher proportion of coexistent leiomyoma (53.1%, P = 0.002). There were no significant differences between group A, B, and C in terms of pain symptoms, endometrial polyps, operation time, and endometriosis fertility index score and other basic characters (p > 0.05). During the follow-up, 59.2% (61/103) patients had clinical pregnancies, and 26.2% (16/61) of them experienced pregnancy loss. Total in vitro fertilization and embryo transfer pregnancy rate was 64.6% (42/65) and spontaneous pregnancy rate was 50.0% (19/38). The Kaplan–Meier curves demonstrated significant lower cumulative pregnancy rate in Group C than Group A and Group B (p = 0.01). Severe obstetric complications such as placenta previa, placenta accreta, preeclampsia, and preterm birth were only found in women with adenomyosis located in the posterior side. In conclusion, types of adenomyosis based on sonographic location had different clinical features and pregnancy outcome. Patients with adenomyosis lesion in both anterior and posterior sides had higher combination of OEM, pelvic adhesion and rAFS score.
A new large-scale gravitational complex discovered in the Gulf of Squillace (central Mediterranean): tectonic implications
Authors: Mangano Giacomo , Ceramicola Silvia , Alves Tiago M. , Zecchin Massimo , Civile Dario , Del Ben Anna , Critelli Salvatore
Published: September 2023

Seismic reflection (2D/3D), borehole and bathymetric data are used to recognize a new gravitational complex in the Gulf of Squillace, Southern Italy, named the Squillace Complex. The complex has a NE-striking headwall connected to a basal detachment formed between Messinian evaporites and Tortonian shales. Its sense of movement changes to a W–E direction in the toe region. In plan view, the Squillace Complex is marked by the presence of sinuous and continuous seafloor scarps, just a few kilometers offshore, over an elongated morphological high. Seismic-well ties reveal that the complex was initiated in the Zanclean (~ 4 Ma) and continued its movement into the Gelasian (~ 2.1 Ma) at an average rate of 1.9 mm/year. Movement slowed down in the Calabrian (middle Pleistocene) and continued until the present day at a lower rate of 0.1 mm/year. Gravitational collapse of the Squillace Complex correlates with discrete contractional/transpressional events affecting the Calabrian region, which caused basin shortening and the temporary arrest of Calabrian Arc migration. These episodes resulted in tectonic uplift in the study area after 0.45 Ma (Late Pleistocene). Conversely, the complex’s slower movement recorded since the Calabrian (middle Pleistocene) is associated with slab rollback of the Ionian plate under the Calabrian Arc.
Joint effects of individual reading skills and word properties on Chinese children’s eye movements during sentence reading
Authors: Yan Ming , Pan Jinger
Published: September 2023

Word recognition during the reading of continuous text has received much attention. While a large body of research has investigated how linguistic properties of words affect eye movements during reading, it remains to be established how individual differences in reading skills affect momentary cognitive processes during sentence reading among typically developing Chinese readers. The present study set out to test the joint influences of word properties and individual reading skills on eye movements during reading among Chinese children. We recorded eye movements of 30 grade 3 (G3) children and 27 grade 5 (G5) children when they read sentences silently for comprehension. Predictors of linear mixed models included word frequency, visual complexity, and launch site distance, in addition to the participants’ offline psychometric performances in rapid naming, morphological awareness, word segmenting, and character recognition. The results showed that word properties affected word recognition during sentence reading in both G3 and G5 children. Moreover, word segmenting predicted the G3 children’s fixation durations and the G5 children’s fixation location, whereas rapid naming predicted the G5 children’s fixation duration. Implications are discussed based on the current findings, in light of how different literacy skills contribute to reading development.
Chaos-based block permutation and dynamic sequence multiplexing for video encryption
Authors: Wen Heping , Lin Yiting , Xie Zhiyu , Liu Tengyu
Published: September 2023

This paper proposes a video security transmission enhancement algorithm based on block permutation and dynamic multiplexing sequences encryption based on 4D autonomous hyperchaotic system. Firstly, we employ the block permutation encryption and diffusion confusion encryption module, which is based on dynamic multiplexing chaotic sequences, to encrypt the plaintext and obtain the ciphertext. Subsequently, the hash value of this round’s ciphertext is utilized to generate the chaotic key, produced by the multiplexing sequence of this round after mathematical processing. Then, the key is used to generate the chaotic sequence to confuse the N-th of the multiplexed sequence, and the next round of multiplexed sequence is obtained. If the current round of chaotic sequence has been completely confused, the chaotic sequence is re-generated to generate a new multiplex sequence by using the key generated by the current round key and the initial key. Finally, the above steps are repeated for the encryption of each frame of the video. Compared with the traditional permutation coding algorithm, it increases the difficulty of estimation or recognition while ensuring efficiency, and effectively improves the avalanche effect of the algorithm. Through frame by frame ciphertext closed-loop feedback, it has the ability to resist known plaintext attack and selected plaintext attack. The results show that the scheme has high security and significant diffusion characteristics, and can effectively resist various common cryptographic attacks.
Integrative blood-based characterization of oxidative mitochondrial DNA damage variants implicates Mexican American’s metabolic risk for developing Alzheimer’s disease
Authors: Reid Danielle Marie , Barber Robert C. , Jones Harlan P. , Thorpe Roland J. , Sun Jie , Zhou Zhengyang , Phillips Nicole R.
Published: September 2023

Alzheimer’s Disease (AD) continues to be a leading cause of death in the US. As the US aging population (ages 65 +) expands, the impact will disproportionately affect vulnerable populations, e.g., Hispanic/Latino population, due to their AD-related health disparities. Age-related regression in mitochondrial activity and ethnic-specific differences in metabolic burden could potentially explain in part the racial/ethnic distinctions in etiology that exist for AD. Oxidation of guanine (G) to 8-oxo-guanine (8oxoG) is a prevalent lesion and an indicator of oxidative stress and mitochondrial dysfunction. Damaged mtDNA (8oxoG) can serve as an important marker of age-related systemic metabolic dysfunction and upon release into peripheral circulation may exacerbate pathophysiology contributing to AD development and/or progression. Analyzing blood samples from Mexican American (MA) and non-Hispanic White (NHW) participants enrolled in the Texas Alzheimer’s Research & Care Consortium, we used blood-based measurements of 8oxoG from both buffy coat PBMCs and plasma to determine associations with population, sex, type-2 diabetes, and AD risk. Our results show that 8oxoG levels in both buffy coat and plasma were significantly associated with population, sex, years of education, and reveal a potential association with AD. Furthermore, MAs are significantly burdened by mtDNA oxidative damage in both blood fractions, which may contribute to their metabolic vulnerability to developing AD.
Clustering microbiome data using mixtures of logistic normal multinomial models
Authors: Fang Yuan , Subedi Sanjeena
Published: September 2023

Discrete data such as counts of microbiome taxa resulting from next-generation sequencing are routinely encountered in bioinformatics. Taxa count data in microbiome studies are typically high-dimensional, over-dispersed, and can only reveal relative abundance therefore being treated as compositional. Analyzing compositional data presents many challenges because they are restricted to a simplex. In a logistic normal multinomial model, the relative abundance is mapped from a simplex to a latent variable that exists on the real Euclidean space using the additive log-ratio transformation. While a logistic normal multinomial approach brings flexibility for modeling the data, it comes with a heavy computational cost as the parameter estimation typically relies on Bayesian techniques. In this paper, we develop a novel mixture of logistic normal multinomial models for clustering microbiome data. Additionally, we utilize an efficient framework for parameter estimation using variational Gaussian approximations (VGA). Adopting a variational Gaussian approximation for the posterior of the latent variable reduces the computational overhead substantially. The proposed method is illustrated on simulated and real datasets.
Changes in health behaviours during the COVID-19 pandemic and effect on weight and obesity among older people in England
Authors: Zhu Jingmin , Di Gessa Giorgio , Zaninotto Paola
Published: September 2023

During COVID-19 lockdown, negative changes in health behaviours have been reported in European older adults. However, less is known about the consequences of these changes on weight gain and obesity, especially in older adults living in England. This study explored the association of health behaviour changes with weight and obesity in English older adults aged 50 years and older, during lockdowns in 2020. We included 4182 participants of the English Longitudinal Study of Ageing COVID-19 sub-study in June/July and Nov/Dec 2020 who also had pre-pandemic data. Perceived changes in health behaviours were regressed on weight and obesity, adjusted for pre-pandemic weight or obesity, and several covariates. Results suggested that less exercise, more sedentariness, eating more and alcohol drinking were associated with a significant increase in weight at both timepoints. Meanwhile, less sedentariness and eating less significantly reduced weight in Nov/Dec 2020. A higher risk of obesity at both timepoints was found in adults sitting, eating, or sleeping more than usual. To conclude, during UK lockdown, older people who engaged in risky health behaviours were at higher risks of weight gain and obesity both in the short run and long term. Considering potential health risks associated with obesity and disruptions in routine lifestyle in the older population even after the pandemic, improved weight management interventions are necessary nationwide.
Evaluating genomic signatures of aging in brain tissue as it relates to Alzheimer’s disease
Authors: Lynch Megan T. , Taub Margaret A. , Farfel Jose M. , Yang Jingyun , Abadir Peter , De Jager Philip L. , Grodstein Francine , Bennett David A. , Mathias Rasika A.
Published: September 2023

Telomere length (TL) attrition, epigenetic age acceleration, and mitochondrial DNA copy number (mtDNAcn) decline are established hallmarks of aging. Each has been individually associated with Alzheimer’s dementia, cognitive function, and pathologic Alzheimer’s disease (AD). Epigenetic age and mtDNAcn have been studied in brain tissue directly but prior work on TL in brain is limited to small sample sizes and most studies have examined leukocyte TL. Importantly, TL, epigenetic age clocks, and mtDNAcn have not been studied jointly in brain tissue from an AD cohort. We examined dorsolateral prefrontal cortex (DLPFC) tissue from N = 367 participants of the Religious Orders Study (ROS) or the Rush Memory and Aging Project (MAP). TL and mtDNAcn were estimated from whole genome sequencing (WGS) data and cortical clock age was computed on 347 CpG sites. We examined dementia, MCI, and level of and change in cognition, pathologic AD, and three quantitative AD traits, as well as measures of other neurodegenerative diseases and cerebrovascular diseases (CVD). We previously showed that mtDNAcn from DLPFC brain tissue was associated with clinical and pathologic features of AD. Here, we show that those associations are independent of TL. We found TL to be associated with β-amyloid levels (beta = − 0.15, p = 0.023), hippocampal sclerosis (OR = 0.56, p = 0.0015) and cerebral atherosclerosis (OR = 1.44, p = 0.0007). We found strong associations between mtDNAcn and clinical measures of AD. The strongest associations with pathologic measures of AD were with cortical clock and there were associations of mtDNAcn with global AD pathology and tau tangles. Of the other pathologic traits, mtDNAcn was associated with hippocampal sclerosis, macroscopic infarctions and CAA and cortical clock was associated with Lewy bodies. Multi-modal age acceleration, accelerated aging on both mtDNAcn and cortical clock, had greater effect size than a single measure alone. These findings highlight for the first time that age acceleration determined on multiple genomic measures, mtDNAcn and cortical clock may have a larger effect on AD/AD related disorders (ADRD) pathogenesis than single measures.
Influence of juvenile hormone analog on behavior in the red imported fire ant, Solenopsis invicta
Authors: Starkey Jesse , Hawkings Chloe , Tamborindeguy Cecilia
Published: September 2023

Division of labor is a hallmark characteristic of social insect colonies. While it is understood that worker differentiation is regulated through either the queen or her brood, the understanding of the physiology behind task regulation varies within social species. Studies in eusocial insects have shown that juvenile hormone (JH) is associated with division of labor and the onset of foraging tasks. Although, outside of a few key species, this interaction has yet to be elucidated in the red imported fire ant, Solenopsis invicta. In this study, we evaluated the role of a JH analog, S-hydroprene in worker task transition in Solenopsis invicta. S-hydroprene was applied to nurses to observe behavioral changes. S-hyroprene application to nurses did not affect phototaxis, but there was a shift in behavior from internal, nest-based behaviors to external, foraging-based behaviors. These results show that JH may be implicated in worker task transition in S. invicta and may function similarly as it does in other eusocial insects.
A system for bioelectronic delivery of treatment directed toward wound healing
Authors: Baniya Prabhat , Tebyani Maryam , Asefifeyzabadi Narges , Nguyen Tiffany , Hernandez Cristian , Zhu Kan , Li Houpu , Selberg John , Hsieh Hao-Chieh , Pansodtee Pattawong , Yang Hsin-ya , Recendez Cynthia , Keller Gordon , Hee Wan Shen , Aslankoohi Elham , Isseroff Roslyn Rivkah , Zhao Min , Gomez Marcella , Rolandi Marco , Teodorescu Mircea
Published: September 2023

The development of wearable bioelectronic systems is a promising approach for optimal delivery of therapeutic treatments. These systems can provide continuous delivery of ions, charged biomolecules, and an electric field for various medical applications. However, rapid prototyping of wearable bioelectronic systems for controlled delivery of specific treatments with a scalable fabrication process is challenging. We present a wearable bioelectronic system comprised of a polydimethylsiloxane (PDMS) device cast in customizable 3D printed molds and a printed circuit board (PCB), which employs commercially available engineering components and tools throughout design and fabrication. The system, featuring solution-filled reservoirs, embedded electrodes, and hydrogel-filled capillary tubing, is assembled modularly. The PDMS and PCB both contain matching through-holes designed to hold metallic contact posts coated with silver epoxy, allowing for mechanical and electrical integration. This assembly scheme allows us to interchange subsystem components, such as various PCB designs and reservoir solutions. We present three PCB designs: a wired version and two battery-powered versions with and without onboard memory. The wired design uses an external voltage controller for device actuation. The battery-powered PCB design uses a microcontroller unit to enable pre-programmed applied voltages and deep sleep mode to prolong battery run time. Finally, the battery-powered PCB with onboard memory is developed to record delivered currents, which enables us to verify treatment dose delivered. To demonstrate the functionality of the platform, the devices are used to deliver H$$^+$$

+

in vivo using mouse models and fluoxetine ex vivo using a simulated wound environment. Immunohistochemistry staining shows an improvement of 35.86% in the M1/M2 ratio of H$$^+$$

+

—treated wounds compared with control wounds, indicating the potential of the platform to improve wound healing.
Effects of season, depth and pre-cultivation fertilizing on Ulva growth dynamics offshore the Eastern Mediterranean Sea
Authors: Zollmann Meiron , Liberzon Alex , Palatnik Ruslana R. , Zilberman David , Golberg Alexander
Published: September 2023

Offshore macroalgae production could provide an alternative source of biomass for food, materials and energy. However, the offshore environment in general, specifically the Eastern Mediterranean Sea (EMS) offshore, is a high energy and low nutrients environment, thus challenging for macroalgae farming. In this study, we experimentally investigated the impact of season, depth, and pre-cultivation fertilization duration on the growth rates and chemical composition of offshoreUlvabiomass, and developed a predictive model tailored to offshore conditions, capable of estimating both biomass growth rate and nitrogen content. Specifically, we measuredUlvabiomass growth rate and internal nitrogen in the nitrogen-poor EMS a few kilometers offshore the Israeli coast at various depths and on-shore pre-cultivation fertilization schedules. Based on these data, we constructed a predictive cultivation model ofUlvaoffshore growth, which allows for the optimization of fertilization requirements for offshore cultivation. This study provides new insights on the effects of seasonality, depth, and pre-cultivation fertilization duration on growth rates and chemical composition of offshoreUlvasp. biomass production.
Chromatic aberration and spectral dependency of extended-range-of-vision intraocular lens technology
Authors: Łabuz Grzegorz , Yan Weijia , Baur Isabella D. , Khoramnia Ramin , Auffarth Gerd U.
Published: September 2023

This study compared the optical quality and chromatic performance of refractive-diffractive intraocular lenses (IOLs) that are designed to extend the range of vision of pseudophakic patients and alter chromatic aberration. Five IOLs were evaluated, Tecnis Synergy and Triumf POD L GF, both intended to compensate for eye's chromatism, as well as Acriva Trinova Pro C—a lens that increases chromatic aberration, and AT Lisa Tri and AcrySof IQ PanOptix. An optical setup composed of a corneal model inducing monochromatic and chromatic aberrations and incorporating various spectral conditions was employed. The two chromatic-aberration correcting IOLs demonstrated the lowest far-focus dispersion, but it was negative only, with the Synergy indicating its ability to reduce eye’s chromatic aberration. Although the Trinova increased far-point chromatism, it was close to the level of the PanOptix, but higher than that of the AT Lisa. All the studied models demonstrated varying optical quality in response to light color. Still, the strongest spectral dependency was associated with achromatizing technology. Therefore, chromatic aberration and wavelength dependency should be considered in IOL optimization and predicting visual function, particularly in non-white spectral conditions.
A remote sensing approach for exploring the dynamics of jellyfish, relative to the water current
Authors: Diamant Roee , Alexandri Talmon , Barak Noga , Lotan Tamar
Published: September 2023

Drifting in large numbers, jellyfish often interfere in the operation of nearshore electrical plants, cause disturbances to marine recreational activity, encroach upon local fish populations, and impact food webs. Understanding the dynamic mechanisms behind jellyfish behavior is of importance in order to create migration models. In this work, we focus on the small-scale dynamics of jellyfish and offer a novel method to accurately track the trajectory of individual jellyfish with respect to the water current. The existing approaches for similar tasks usually involve a surface float tied to the jellyfish for location reference. This operation may induce drag on the jellyfish, thereby affecting its motion. Instead, we propose to attach an acoustic tag to the jellyfish’s bell and then track its geographical location using acoustic beacons, which detect the tag’s emissions, decode its ID and depth, and calculate the tag’s position via time-difference-of-arrival acoustic localization. To observe the jellyfish’s motion relative to the water current, we use a submerged floater that is deployed together with the released tagged jellyfish. Being Lagrangian on the horizontal plane while maintaining an on-demand depth, the floater drifts with the water current; thus, its trajectory serves as a reference for the current’s velocity field. Using an acoustic modem and a hydrophone mounted to the floater, the operator from the deploying boat remotely changes the depth of the floater on-the-fly, to align it with that of the tagged jellyfish (as reported by the jellyfish’s acoustic tag), thereby serving as a reference for the jellyfish’s 3D motion with respect to the water current. We performed a proof-of-concept to demonstrate our approach over three jellyfish caught and tagged in Haifa Bay, and three corresponding floaters. The results present different dynamics for the three jellyfish, and show how they can move with, and even against, the water current.
A powered simple walking model explains the decline in propulsive force and hip flexion torque compensation in human gait
Authors: Ohtsu Hajime , Hase Kazunori , Sakoda Kouta , Aoi Shinya , Kita Shunsuke , Ogaya Shinya
Published: September 2023

Excessive hip flexion torque to prioritize leg swings in the elderly is likely to be a factor that reduces their propulsive force and gait stability, but the mechanism is not clear. To understand the mechanism, we investigated how propulsive force, hip flexion torque, and margin of stability (MoS) change when only the hip spring stiffness is increased without changing the walking speed in the simple walking model, and verified whether the relationship holds in human walking. The results showed that at walking speeds between 0.50 and 1.75 m/s, increasing hip spring stiffness increased hip flexion torque and decreased the propulsive force and MoS in both the model and human walking. Furthermore, it was found that the increase in hip flexion torque was explained by the increase in spring stiffness, and the decreases in the propulsive force and MoS were explained by the increase in step frequency associated with the increase in spring stiffness. Therefore, the increase in hip flexion torque likely decreased the propulsive force and MoS, and this mechanism was explained by the intervening hip spring stiffness. Our findings may help in the control design of walking assistance devices, and in improving our understanding of elderly walking strategies.
Pet ownership and maintenance of cognitive function in community-residing older adults: evidence from the Baltimore Longitudinal Study of Aging (BLSA)
Authors: Friedmann Erika , Gee Nancy R. , Simonsick Eleanor M. , Kitner-Triolo Melissa H. , Resnick Barbara , Adesanya Ikmat , Koodaly Lincy , Gurlu Merve
Published: September 2023

Pet ownership has been associated with reduced deterioration in physical health as older adults age; little research focused on deterioration in cognitive function. We examine the relationship of pet, dog, cat ownership, and dog walking to changes in cognitive function among 637 generally healthy community-dwelling older adults (185 pet owners) aged 50–100 years (M = 68.3, SD = 9.6) within the BLSA. Cognitive assessments every 1–4 years over 1–13 years (M = 7.5, SD = 3.6) include the California Verbal Learning (Immediate, Short, Long Recall); Benton Visual Retention; Trail-Making (Trails A, B, B-A); Digit Span; Boston Naming (Naming); and Digit Symbol Substitution (Digit Symbol) Tests. In linear mixed models, deterioration in cognitive function with age was slower for pet owners than non-owners (Immediate, Short, Long Recall; Trails A,B,B-A; Naming; Digit Symbol); dog owners than non-owners (Immediate, Short Recall; Trails A,B; Naming; Digit Symbol); and cat owners than non-owners (Immediate, Short, Long Recall; Naming), controlling for age and comorbidities. Among dog owners (N = 73) walkers experienced slower deterioration than non-walkers (Trails B, B-A; Short Recall). All ps ≤ 0.05. We provide important longitudinal evidence that pet ownership and dog walking contribute to maintaining cognitive function with aging and the need to support pet ownership and dog walking in design of senior communities and services.
Modulation of colonic immunometabolic responses during Clostridioides difficile infection ameliorates disease severity and inflammation
Authors: Tubau-Juni Nuria , Bassaganya-Riera Josep , Leber Andrew J. , Alva Sameeksha S. , Baker Ryan , Hontecillas Raquel
Published: September 2023

Clostridioides difficile infection (CDI) is the leading cause of antibiotic-associated diarrhea, and its clinical symptoms can span from asymptomatic colonization to pseudomembranous colitis and even death. The current standard of care for CDI is antibiotic treatment to achieve bacterial clearance; however, 15 to 35% of patients experience recurrence after initial response to antibiotics. We have conducted a comprehensive, global colonic transcriptomics analysis of a 10-day study in mice to provide new insights on the local host response during CDI and identify novel host metabolic mechanisms with therapeutic potential. The analysis indicates major alterations of colonic gene expression kinetics at the acute infection stage, that are restored during the recovery phase. At the metabolic level, we observe a biphasic response pattern characterized by upregulated glycolytic metabolism during the peak of inflammation, while mitochondrial metabolism predominates during the recovery/healing stage. Inhibition of glycolysis via 2-Deoxy-d-glucose (2-DG) administration during CDI decreases disease severity, protects from mortality, and ameliorates colitis in vivo. Additionally, 2-DG also protects intestinal epithelial cells from C. difficile toxin damage, preventing loss of barrier integrity and secretion of proinflammatory mediators. These data postulate the pharmacological targeting of host immunometabolic pathways as novel treatment modalities for CDI.
Characterising resting patterns of mother-calf humpback whale groups in a semi-enclosed embayment along the Australian east coast migration pathway
Authors: Jones Alexandra , Bruce Eleanor , Cato Douglas H.
Published: September 2023

On migration from low latitude breeding grounds to high latitude feeding grounds, humpback whale mothers and calves spend time resting in coastal embayments. Unlike other areas where resting has been documented, Jervis Bay, on Australia’s east coast, is remote from both breeding and feeding grounds, and provides a unique opportunity to compare resting behaviour observed within a semi-enclosed embayment to observations offshore. Land-based, and UAV surveys were conducted in Jervis Bay in 2018, 2019, and 2021. We show that (i) a disproportionately high percentage of groups with a calf enter Jervis Bay during the southbound migration, (ii) travelling speeds are significantly slower in the Bay compared to offshore, indicating resting behaviour, and (iii) aerial observations highlight resting and nurturing behaviour. Subsequently, we conclude that Jervis Bay is an important area for resting mother-calf humpback whale groups. Comparison with reports of resting behaviour during migration in areas nearer the breeding grounds shows commonalities that characterise resting behaviour in mothers and calves. This characterisation will allow improved monitoring and management of humpback whales in nearshore embayments during a critical stage of calf development, particularly those with increased anthropogenic activities.
Association between endothelial nitric oxide synthase (eNOS) gene variants and nitric oxide production in preeclampsia: a case–control study in Ghana
Authors: Fondjo Linda Ahenkorah , Awuah Enoch Ofori , Sakyi Samuel Asamoah , Senu Ebenezer , Detoh Eric
Published: September 2023

Evidence suggests that a major cause of PE is endothelial dysfunction emanating from the reduced bioavailability of Nitric oxide (NO). Variants of endothelial nitric oxide synthase (eNOS) gene may lead to decreased NO levels. We explored the association between eNOS gene variants and nitric oxide levels among preeclamptic women in the Ghanaian population. This case–control study included 75 preeclamptic women and 75 healthy normotensive pregnant women attending antenatal care at the Nkawie-Toase Government Hospital, Ghana. A well-structured questionnaire was used to collect socio-demographic, obstetric and clinical data. Blood was obtained for DNA extraction; the gene variants were determined using PCR and RFLP. Preeclamptic women had significantly lower NO concentration compared to the normotensives (p < 0.0001) and was significantly different between VNTR variants (p < 0.0001). A significant difference in VNTR intron 4 distribution was also observed between the preeclamptic and normotensive women with 4c4c” (12.0%) and “4a4c” (1.3%) genotypes found predominantly in preeclamptic women (p < 0.0001). There was significantly higher distribution of “TC” genotype in preeclamptic women (44.0%) compared to normotensives (22.7%) (p = 0.019). However, possessing “4a4b” (cOR: 0.17, 95% CI 0.04–0.64) and “4b4b” (cOR: 0.09, 95% CI 0.02–0.38) significantly decreased the likelihood of experiencing preeclampsia by 83% and 91% respectively. Nitric oxide is reduced in preeclamptic women. NO levels in preeclampsia are altered by VNTR intron 4 variants but not T786C variants. Possessing VNTR intron 4 “4b” allele decreases the risk of PE while the “4c” allele increases the risk of PE. There is the need for eNOS variant screening and nitric oxide estimation among pregnant women for early prediction of women at risk of preeclampsia.
Clinical value of vestibulo-ocular reflex in the differentiation of spinocerebellar ataxias
Authors: Kim Jae-Myung , Nam Tai-Seung , Choi Seong-Min , Kim Byeong C. , Lee Seung-Han
Published: September 2023

The diagnosis of spinocerebellar ataxia (SCA) currently depends upon genetic testing. Although genetic testing for SCA is highly specific, clinical parameters for the differentiation of SCAs are still insufficient. We aimed to assess the vestibulo-ocular reflex (VOR) parameters of various SCA subtypes to determine whether they have substantial value in differential diagnosis. We consecutively enrolled 33 genetically confirmed SCA patients (SCA2 = 8, SCA3 = 6, SCA6 = 10, SCA7 = 9). Normative data were obtained from 36 age- and gender-matched healthy controls. Quantitative indicators of VOR were measured using video head impulse test (HIT) and combined ocular motor dysfunctions were investigated using video-oculography. Compared with the control group, the VOR gains in SCA2 were relatively spared, but were markedly decreased for all six canals in SCA3. The VOR gains for the posterior canals (PCs) were significantly decreased in SCA6, and for both vertical canals were decreased in SCA7. The VOR gains for the horizontal canals in SCA3 were negatively correlated with disease severity (R = -0.900, p = 0.037). Abnormal catch-up saccades were common in SCA3 and SCA6, rare in SCA7 and absent in SCA2. Spontaneous, headshaking-induced, and positional nystagmus were only documented in SCA6. SCA3 and SCA6 commonly showed horizontal gaze-evoked nystagmus, but SCA2 and SCA7 had characteristic saccadic slowing without gaze-evoked nystagmus. VOR impairments are common in SCAs, but their patterns vary depending on subtype. In addition to ocular motor characteristics, distinctive VOR performance for each subtype using video HIT may aid the differential diagnosis of the SCA genotypes.
Asarinin attenuates bleomycin-induced pulmonary fibrosis by activating PPARγ
Authors: Zeng Qian , Zhou Ting-ting , Huang Wen-jie , Huang Xiao-ting , Huang Lei , Zhang Xiao-hua , Sang Xiao-xue , Luo Yu-yang , Tian Yu-mei , Wu Bin , Liu Lin , Luo Zi-qiang , He Bin , Liu Wei , Tang Si-yuan
Published: September 2023

Idiopathic pulmonary fibrosis (IPF) is a chronic progressive interstitial lung disease that lacks effective treatment modalities. Once patients are diagnosed with IPF, their median survival is approximately 3–5 years. PPARγ is an important target for the prevention and treatment of pulmonary fibrosis. Asarinin is a lignan compound that can be extracted from food plant Asarum heterotropoides. In this study, we investigated the therapeutic effects of asarinin in a pulmonary fibrosis model constructed using bleomycin in mice and explored the underlying mechanisms. Intraperitoneal administration of asarinin to mice with pulmonary fibrosis showed that asarinin effectively attenuated pulmonary fibrosis, and this effect was significantly inhibited by the PPARγ inhibitor GW9662. Asarinin inhibited TGF-β1-induced fibroblast-to-myofibroblast transition in vitro, while GW9662 and PPARγ gene silencing significantly inhibited this effect. In addition, asarinin inhibited not only the canonical Smad pathway of TGF-β but also the non-canonical AKT and MAPK pathways by activating PPARγ. Our study demonstrates that asarinin can be used as a therapeutic agent for pulmonary fibrosis, and that PPARγ is its key target.
Distinct N7-methylguanosine profiles of circular RNAs in drug-resistant acute myeloid leukemia
Authors: Fu Jinqiu , Si Libo , Zhou Yao , Li Dong , Wang Ran
Published: September 2023

Post-transcriptional methylation modifications, such as the N7-methylguanosine (m7G) modification, are increasingly acknowledged for their role in the development and resistance to chemotherapy in acute myeloid leukemia (AML). This study employed MeRIP-seq technology to investigate the m7G sites within circular RNAs (circRNAs) derived from human AML cells and drug-resistant AML cells, in order to identify these sites more comprehensively. In addition, a detailed analysis of the relationship between m7G and drug-resistant AML was conducted. The bioinformatics analysis was utilized to predict the functions of specific methylated transcripts. The findings revealed a significant difference in m7G level between AML cells and drug-resistant AML cells, suggesting a potentially critical role of m7G in circRNAs in drug-resistant AML development. The methylation of M7G could affect the circRNA-miRNA-mRNA co-expression during the development of AML resistance, which could further influence the regulation of resistance-associated target genes in AML. Furthermore, gene ontology analysis indicated that the distinct distribution pattern of circRNAs with m7G methylation in drug-resistant AML cells was correlated with metabolism-related pathways. These results suggested a potential association between drug-resistant AML and m7G methylation of circRNAs. Moreover, the results revealed a novel role of m7G RNA methylation in circRNAs in the progression of AML chemoresistance.