bluuebunny/crossref_metadata_2025 huggingface dataset:
Wear and Breakage Detection of Integral Spiral End Milling Cutters Based on Machine Vision
Authors: Wei Wenming , Yin Jia , Zhang Jun , Zhang Huijie , Lu Zhuangzhuang
Published: September 2021

Tool wear and breakage detection technologies are of vital importance for the development of automatic machining systems and improvement in machining quality and efficiency. The monitoring of integral spiral end milling cutters, however, has rarely been investigated due to their complex structures. In this paper, an image acquisition system and image processing methods are developed for the wear and breakage detection of milling cutters based on machine vision. The image acquisition system is composed of three light sources and two cameras mounted on a moving frame, which renders the system applicable in cutters of different dimensions and shapes. The images captured by the acquisition system are then preprocessed with denoising and contrast enhancing operations. The failure regions on the rake face, flank face and tool tip of the cutter are extracted with the Otsu thresholding method and the Markov Random Field image segmentation method afterwards. Eventually, the feasibility of the proposed image acquisition system and image processing methods is demonstrated through an experiment of titanium alloy machining. The proposed image acquisition system and image processing methods not only provide high quality detection of the integral spiral end milling cutter but can also be easily converted to detect other cutting systems with complex structures.
Maghemite (γ-Fe2O3) and γ-Fe2O3-TiO2 Nanoparticles for Magnetic Hyperthermia Applications: Synthesis, Characterization and Heating Efficiency
Authors: Lemine O. M. , Madkhali Nawal , Alshammari Marzook , Algessair Saja , Gismelseed Abbasher , El Mir Lassad , Hjiri Moktar , Yousif Ali A. , El-Boubbou Kheireddine
Published: September 2021

In this report, the heating efficiencies of γ-Fe2O3 and hybrid γ-Fe2O3-TiO2 nanoparticles NPs under an alternating magnetic field (AMF) have been investigated to evaluate their feasible use in magnetic hyperthermia. The NPs were synthesized by a modified sol-gel method and characterized by different techniques. X-ray diffraction (XRD), Mössbauer spectroscopy and electron microscopy analyses confirmed the maghemite (γ-Fe2O3) phase, crystallinity, good uniformity and 10 nm core sizes of the as-synthesized composites. SQUID hysteresis loops showed a non-negligible coercive field and remanence suggesting the ferromagnetic behavior of the particles. Heating efficiency measurements showed that both samples display high heating potentials and reached magnetic hyperthermia (42 °C) in relatively short times with shorter time (~3 min) observed for γ-Fe2O3 compared to γ-Fe2O3-TiO2. The specific absorption rate (SAR) values calculated for γ-Fe2O3 (up to 90 W/g) are higher than that for γ-Fe2O3-TiO2 (~40 W/g), confirming better heating efficiency for γ-Fe2O3 NPs. The intrinsic loss power (ILP) values of 1.57 nHm2/kg and 0.64 nHm2/kg obtained for both nanocomposites are in the range reported for commercial ferrofluids (0.2–3.1 nHm2/kg). Finally, the heating mechanism responsible for NP heat dissipation is explained concluding that both Neel and Brownian relaxations are contributing to heat production. Overall, the obtained high heating efficiencies suggest that the fabricated nanocomposites hold a great potential to be utilized in a wide spectrum of applications, particularly in magnetic photothermal hyperthermia treatments.
Effect of Chloride Ions Concentrations to Breakdown the Passive Film on Rebar Surface Exposed to L-Arginine Containing Pore Solution
Authors: Singh Jitendra Kumar , Mandal Soumen , Lee Han-Seung , Yang Hyun-Min
Published: September 2021

In the present study, 0.115 M L-arginine (LA) has been used as an eco-friendly inhibitor in simulated concrete pore solutions (SP-0) in order to form passive films on a steel rebar–solution interface until 144 h. Hence, 0.51 (SP-1) and 0.85 M NaCl (SP-2) were added in LA containing SP-0 solution to breakdown the passive film and to initiate corrosion reactions. The electrochemical results show that the charge transfer resistance (Rct) of steel rebar exposed to SP-1 and SP-2 solutions increased with respect to immersion periods. The sample exposed to the SP-2 solution initiated the corrosion reaction at the steel rebar–solution interface after 24 h of NaCl addition and formed pits; on the other hand, the sample without NaCl added, i.e., SP-0, showed agglomeration and dense morphology of corrosion products.
Effect of Thermo-Mechanical Treatment on the Microstructure and Tensile Properties of the Fe-22Cr-5Al-0.1Y Alloy
Authors: Che Hongyan , Zhai Yazhong , Yan Yingjie , Chen Yongqing , Qin Wei , Wang Tiejun , Cao Rui
Published: September 2021

Oxide dispersion strengthened ferritic steel is considered an important structural material in fusion reactors due to its excellent resistance to radiation and oxidation. Fine and dispersed oxides can be introduced into the matrix via the powder metallurgy process. In the present study, large grain sizes and prior particle boundaries (PPBs) formed in the FeCrAlY alloy prepared via powder metallurgy. Thermo-mechanical treatment was conducted on the FeCrAlY alloy. Results showed that microstructure was optimized: the average grain diameter decreased, the PPBs disappeared, and the distribution of oxides dispersed. Both ultimate tensile strength and elongation improved, especially the average elongation increased from 0.5% to 23%.
Experimental Investigation on Dry Routing of CFRP Composite: Temperature, Forces, Tool Wear, and Fine Dust Emission
Authors: Elgnemi Tarek , Songmene Victor , Kouam Jules , Jun Martin B.G. , Samuel Agnes Marie
Published: September 2021

This article presents the influence of machining conditions on typical process performance indicators, namely cutting force, specific cutting energy, cutting temperature, tool wear, and fine dust emission during dry milling of CFRPs. The main goal is to determine the machining process window for obtaining quality parts with acceptable tool performance and limited dust emission. For achieving this, the cutting temperature was examined using analytical and empirical models, and systematic cutting experiments were conducted to assess the reliability of the theoretical predictions. A full factorial design was used for the experimental design. The experiments were conducted on a CNC milling machine with cutting speeds of 10,000, 15,000, and 20,000 rpm and feed rates of 2, 4, and 6 µm/tooth. Based on the results, it was ascertained that spindle speed significantly affects the cutting temperature and fine particle emission while cutting force, specific cutting energy, and tool wear are influenced by the feed rate. The optimal conditions for cutting force and tool wear were observed at a cutting speed of 10,000 rpm. The cutting temperature did not exceed the glass transition temperature for the cutting speeds tested and feed rates used. The fine particles emitted ranged from 0.5 to 10 µm aerodynamic diameters with a maximum concentration of 2776.6 particles for those of 0.5 µm diameters. Finally, results of the experimental optimization are presented, and the model is validated. The results obtained may be used to better understand specific phenomena associated with the milling of CFRPs and provide the means to select effective milling parameters to improve the technology and economics of the process.
Piezoelectric Based Touch Sensing for Interactive Displays—A Short Review
Authors: Liu Ziting , Fu Zhe
Published: September 2021

Interactive display is an important part of electronic devices. It is widely used in smartphones, laptops, and industrial equipment. To achieve 3-dimensional detection, the piezoelectric touch panel gains great popularity for its advantages of high sensitivity, low cost, and simple structure. In order to help readers understand the basic principles and the current technical status, this article introduces the work principles of the piezoelectric touch panel, widely-used piezoelectric materials and their characteristics, as well as the applications of the piezoelectric touch panel. The challenges and future trends are also discussed.
On the Design of a Class of Rotary Compressors Using Bayesian Optimization
Authors: Lu Kui , Phung Truong H. , Sultan Ibrahim A.
Published: September 2021

The optimization process of compressors is usually regarded as a ‘black-box’ problem, in which the mathematical form underlying the relationship between design parameters and the design objective is impractical and costly to be obtained. To solve the ‘black-box’ problem, Bayesian optimization has been proven as an accurate and efficient method. However, the application of such a method in the design of compressors is rarely discussed, particularly no work has been reported in terms of the positive displacement type compressor. Therefore, this paper aims to introduce the Bayesian optimization to the design of positive displacement compressors through the optimization process of the novel limaçon compressor. In this paper, a two-stage optimization process is presented, in which the first stage optimizes the geometric parameters as per design requirements and the second stage focuses on revealing an optimum setting of port geometries that improves machine performance. A numerical illustration is offered to prove the validity of the presented approach.
Vibrodiagnostics Faults Classification for the Safety Enhancement of Industrial Machinery
Authors: Zuth Daniel , Blecha Petr , Marada Tomas , Huzlik Rostislav , Tuma Jiri , Maradova Karla , Frkal Vojtech
Published: September 2021

The current digitization of industrial processes is leading to the development of smart machines and smart applications in the field of engineering technologies. The basis is an advanced sensor system that monitors selected characteristic values of the machine. The obtained data need to be further analysed, correctly interpreted, and visualized by the machine operator. Thus the machine operator can gain a sixth sense for keeping the machine and the production process in a suitable condition. This has a positive effect on reducing the stress load on the operator in the production of expensive components and in monitoring the safe condition of the machine. The key element here is the use of a suitable classification model for data evaluation of the monitored machine parameters. The article deals with the comparison of the success rate of classification models from the MATLAB Classification Learner App. Classification models will compare data from the frequency and time domain, the data source is the same. Both data samples are from real measurements on the CNC vertical machining center (CNC-Computer Numerical Control). Three basic states representing machine tool damage are recognized. The data are then processed and reduced for the use of the MATLAB Classification Learner app, which creates a model for recognizing faults. The article aims to compare the success rate of classification models when the data source is a dataset in time or frequency domain and combination.
Plant Sterol Clustering Correlates with Membrane Microdomains as Revealed by Optical and Computational Microscopy
Authors: Tang Ling , Li Yang , Zhong Cheng , Deng Xin , Wang Xiaohua
Published: September 2021

Local inhomogeneities in lipid composition play a crucial role in the regulation of signal transduction and membrane traffic. This is particularly the case for plant plasma membrane, which is enriched in specific lipids, such as free and conjugated forms of phytosterols and typical phytosphingolipids. Nevertheless, most evidence for microdomains in cells remains indirect, and the nature of membrane inhomogeneities has been difficult to characterize. We used a new push–pull pyrene probe and fluorescence lifetime imaging microscopy (FLIM) combined with all-atom multiscale molecular dynamics simulations to provide a detailed view on the interaction between phospholipids and phytosterol and the effect of modulating cellular phytosterols on membrane-associated microdomains and phase separation formation. Our understanding of the organization principles of biomembranes is limited mainly by the challenge to measure distributions and interactions of lipids and proteins within the complex environment of living cells. Comparing phospholipids/phytosterol compositions typical of liquid-disordered (Ld) and liquid-ordered (Lo) domains, we furthermore show that phytosterols play crucial roles in membrane homeostasis. The simulation work highlights how state-of-the-art modeling alleviates some of the prior concerns and how unrefuted discoveries can be made through a computational microscope. Altogether, our results support the role of phytosterols in the lateral structuring of the PM of plant cells and suggest that they are key compounds for the formation of plant PM microdomains and the lipid-ordered phase.
Influence of High-Pressure Torsion on the Microstructure and Microhardness of Additively Manufactured 316L Stainless Steel
Authors: Mohd Yusuf Shahir Mohd , Chen Ying , Gao Nong
Published: September 2021

High-pressure torsion (HPT) is known as an effective severe plastic deformation (SPD) technique to produce bulk ultrafine-grained (UFG) metals and alloys by the application of combined compressive force and torsional shear strains on thin disk samples. In this study, the microstructures and microhardness evolution of an additively manufactured (AM) 316L stainless steel (316L SS) processed through 5 HPT revolutions are evaluated at the central disk area, where the effective shear strains are relatively low compared to the peripheral regions. Scanning electron microscopy (SEM) analysis showed that the cellular network sub-structures in AM 316L SS were destroyed after 5 HPT revolutions. Transmission electron microscopy (TEM) observations revealed non-equilibrium ultrafine grained (UFG) microstructures (average grain size: ~115 nm) after 5 revolutions. Furthermore, energy dispersive x-ray spectroscopy (EDX) analysis suggested that spherical Cr-based nano-silicates are also found in the as-received condition, which are retained even after HPT processing. Vickers microhardness (HV) measurements indicated significant increase in average hardness values from ~220 HV before HPT processing to ~560 HV after 5 revolutions. Quantitative X-ray diffraction (XRD) patterns exhibit a considerable increase in dislocation density from ~0.7 × 1013 m−2 to ~1.04 × 1015 m−2. The super-high average hardness increment after 5 HPT revolutions is predicted to be attributed to the UFG grain refinement, significant increase in dislocation densities and the presence of the Cr-based nano-silicates, according to the model established based on the linear additive theory.
The Effect of Plume Generated on the Microstructural Behavior of the Weld Mixed Zone in High-Speed Laser Dissimilar Welding
Authors: Lee Su-Jin , Katayama Seiji , Kim Jong-Do , Suh Jeong
Published: September 2021

Dissimilar laser welding has been researched to combine the excellent anticorrosion and high strength properties of Ti and the low weight and cost of Al. However, when welding dissimilar Al and Ti sheets, many kinds of intermetallic compound are easily generated. Therefore, intermetallic compounds and differences in material properties make joining such dissimilar metals very difficult. Previous studies clarified that ultra-high welding speed could suppress the weld defects. To elucidate the mechanism of Al and Ti dissimilar laser welding, material behavior of the weld fusion zone and components of fume generated during the ultra-high speed welding process were observed and analyzed using energy dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), high speed cameras, and a spectrometer. The results show that the atom movement of Al and Ti in the weld plume affects the behavior of elemental components distributed in the weld fusion zone.
Structure Evolution of Ni36Al27Co37 Alloy in the Process of Mechanical Alloying and Plasma Spheroidization
Authors: Mazeeva Alina K. , Kim Artem , Ozerskoi Nikolay E. , Shamshurin Aleksey I. , Razumov Nikolay G. , Nazarov Denis V. , Popovich Anatoliy A.
Published: September 2021

In this paper, a novel approach to obtain a ferromagnetic material for smart applications was implied. A combination of mechanical alloying (MA) and plasma spheroidization (PS) was applied to produce Ni36Al27Co37 spherical powder. Then its structure was systematically studied. It was shown that homogenization of the structure occurs due to mechanism of layered structure formation. The dependence of the lamella thickness on the energy dose input at MA was defined. It was found that 14.7 W⋅h/g is sufficient to obtain lamella thickness of 1 μm and less. The low-energy mode of a planetary mill with rotation speeds of the main disk/bowl of 150/−300 rpm makes it possible to achieve a uniform element distribution upon a minimal amount of impurity. During MA in an attritor Ni3Al-type intermetallic compounds are formed that result in more intensive degradation in particle size. Plasma spheroidization of the powder after MA allowed obtaining Ni36Al27Co37 spherical powder. The powder had a fine β + γ-structure. The particle size distribution remains almost unchanged compared to the MA stage. Coercivity of the powder is 79 Oe. The powder obtained meets the requirements of selective laser melting technology, but also can be utilized as a functional filler in various magnetic composites.
Numerical Investigations of Shape Memory Alloy Fatigue
Authors: Dornelas Vanderson M. , Oliveira Sergio A. , Savi Marcelo A. , Pacheco Pedro Manuel Calas Lopes
Published: September 2021

This work deals with numerical investigations of the functional and structural fatigue on shape memory alloys (SMAs). A thermodynamically consistent, three-dimensional constitutive model is employed, adopting a continuum damage perspective. Fatigue life is predicted by considering a macroscopic model. Numerical simulations are compared with experimental data taken from the literature to demonstrate the model’s ability to capture the general thermomechanical behavior of SMAs subjected to different loading conditions. Uniaxial and torsion tests are discussed; thermal loads are also analyzed considering the influence of the maximum temperature on the fatigue life of SMAs. Cyclic degradation of the shape memory effect is investigated in the sequence. Results show that numerical simulations are in good agreement with the experimental data, including the fatigue life estimation.
Kinetics Study on the Modification Process of Al2O3 Inclusions in High-Carbon Hard Wire Steel by Magnesium Treatment
Authors: Xiong Xingqiang , Li Changrong , Xi Zuobing , Chen Lu
Published: September 2021

The aim of the experiment in this work is to modify the Al2O3 inclusions in high-carbon hard wire steel by magnesium treatment. The general evolution process of inclusions in steel is: Al2O3 → MgO·Al2O3(MA) → MgO. The unreacted core model was used to study the modification process of inclusions. The results show that the complete modification time (tf) of inclusions is significantly shortened by the increase of magnesium content in molten steel. For Al2O3 inclusions with radius of 1 μm and Mg content in the range of 0.0005–0.0055%, the modification time of Al2O3 inclusions to MA decreased from 755 s to 25 s, which was reduced by 730 s. For Al2O3 inclusions with a radius of 1.5 μm and Mg content in the range of 0.001–0.0035%, the Al2O3 inclusions were completely modified to MgO inclusions from 592 s to 55 s. The Mg content in the molten steel increased 3.4-fold, and the time for complete modification of inclusions was shortened by about 10-fold. With the increase of Al and O content in molten steel, the complete modification time increased slightly, but the change was small. At the same time, the larger the radius of the unmodified inclusion is, the longer the complete modification time is. The tf of Al2O3 inclusions with a radius of 1 μm when modified to MA is 191 s, and the tf of Al2O3 inclusions with a radius of 2 μm when modified to MA is 765 s. According to the boundary conditions and the parameters of the unreacted core model, the MgO content in inclusions with different radius is calculated. The experimental results are essentially consistent with the kinetic calculation results.
Tribological Behavior of Boronized Fe40Mn20Cr20Ni20 High-Entropy Alloys
Authors: Guo Xin , Jin Xi , Shi Xiaohui , Yang Huijun , Zhang Min , Qiao Junwei
Published: September 2021

The tribological behavior of hot-rolled and boronized Fe40Mn20Cr20Ni20 high-entropy alloys (HEAs) sliding against a Si3N4 ball was investigated in the air, deionized water and seawater. The results showed that the hot-rolled Fe40Mn20Cr20Ni20 HEA was composed of an FCC (face-centered cubic) phase. In addition, the boronized HEA was composed of a great number of borides, including CrB, FeB, MnB, Fe2B, Fe3B and MnB2. The hardness increased from 139 HV to 970 HV after boronizing. In air, the wear rate decreased from 4.51 × 10−4 mm3/Nm to 0.72 × 10−4 mm3/Nm after boronizing. The wear mechanism transformed from abrasive wear and oxidative wear to the polishing effect. After boronizing, in the deionized water, the wear rate decreased from 1.27 × 10−4 mm3/Nm to 8.43 × 10−5 mm3/Nm. The wear mechanism transformed from abrasive wear and delamination wear to delamination wear. In the seawater, the wear rate decreased by about ten times that of hot-rolled alloy.
Continuous Cooling Transformation of Under-Cooled Austenite of SXQ500/550DZ35 Hydropower Steel
Authors: Tang Zhenglei , Guo Ran , Zhang Yang , Liu Zhen , Lu Yuezhang , Wang Fuming
Published: September 2021

The expansion curves of the continuous cooling transformation of undercooled austenite of SXQ500/550DZ35 hydropower steel at different heating temperatures and cooling rates were measured by use of a DIL805A dilatometer. Combined with metallography and Vickers hardness measurement, the continuous cooling transformation diagrams (CCT) of the studied steel under two different states were determined. The results show that in the first group of tests, after the hot-rolled specimens were austenitized at 920 °C, when the cooling rate was below 1 °C·s−1, the microstructure was composed of ferrite (F), pearlite (P) and bainite (B). With the cooling rates between 1 °C·s−1 and 5 °C·s−1, the microstructure was mainly bainite, and martensite (M) formed as the cooling rate reached 5 °C·s−1. When the cooling rate was up to 10 °C·s−1, the microstructure was completely martensite and the hardness value increased significantly. In the second group of tests, after the hot-rolled specimens were quenched at 920 °C and then heated at an intercritical temperature of 830 °C, in comparison with the first group of tests, and except for additional undissolved ferrites in each cooling rate range, the other microstructure types were basically the same. Due to the existence of undissolved ferrite, the microstructures of the specimens heated at intercritical temperatures were much finer, and the toughness values at low temperatures were better.
Knockout of the Amino Acid Transporter SLC6A19 and Autoimmune Diabetes Incidence in Female Non-Obese Diabetic (NOD) Mice
Authors: Waters Matthew F. , Delghingaro-Augusto Viviane , Javed Kiran , Dahlstrom Jane E. , Burgio Gaetan , Bröer Stefan , Nolan Christopher J.
Published: September 2021

High protein feeding has been shown to accelerate the development of type 1 diabetes in female non-obese diabetic (NOD) mice. Here, we investigated whether reducing systemic amino acid availability via knockout of the Slc6a19 gene encoding the system B(0) neutral amino acid transporter AT1 would reduce the incidence or delay the onset of type 1 diabetes in female NOD mice. Slc6a19 gene deficient NOD mice were generated using the CRISPR-Cas9 system which resulted in marked aminoaciduria. The incidence of diabetes by week 30 was 59.5% (22/37) and 69.0% (20/29) in NOD.Slc6a19+/+ and NOD.Slc6a19−/− mice, respectively (hazard ratio 0.77, 95% confidence interval 0.41–1.42; Mantel-Cox log rank test: p = 0.37). The median survival time without diabetes was 28 and 25 weeks for NOD.Slc6a19+/+ and NOD.Slc6a19−/− mice, respectively (ratio 1.1, 95% confidence interval 0.6–2.0). Histological analysis did not show differences in islet number or the degree of insulitis between wild type and Slc6a19 deficient NOD mice. We conclude that Slc6a19 deficiency does not prevent or delay the development of type 1 diabetes in female NOD mice.
New Approach for the Construction and Calibration of Gas-Tight Setups for Biohydrogen Production at the Small Laboratory Scale
Authors: Autenrieth Caroline , Shaw Shreya , Ghosh Robin
Published: September 2021

Biohydrogen production in small laboratory scale culture vessels is often difficult to perform and quantitate. One problem is that commonly used silicon tubing and improvised plastic connections used for constructing apparatus are cheap and easy to connect but are generally not robust for gases such as hydrogen. In addition, this type of apparatus presents significant safety concerns. Here, we demonstrate the construction of hydrogen-tight apparatus using a commercially available modular system, where plastic tubing and connections are made of explosion-proof dissipative plastic material. Using this system, we introduce a gas chromatograph calibration procedure, which can be easily performed without necessarily resorting to expensive commercial gas standards for the calibration of hydrogen gas concentrations. In this procedure, the amount of hydrogen produced by the reaction of sodium borohydride with water in a closed air-filled bottle is deduced from the observed decrease of the oxygen partial pressure, using the ideal gas law. Finally, the determined calibration coefficients and the gas-tight apparatus are used for the analysis of simultaneous oxygen consumption and hydrogen production of the purple photosynthetic bacterium, Rhodospirillum rubrum, during semi-aerobic growth in the dark.
Experimental Research on Deep-And-Narrow Micromilled Grooves Using a Self-Fabricated PCD Micro-Cutter
Authors: Han Jinjin , Ma Rui , Hao Xiuqing , Kong Linglei , Chen Ni , Li Liang , He Ning
Published: September 2021

Deep-and-narrow micro-grooves are the common functional structures of miniature parts. The fabrication of the micromilled grooves with high quality and accuracy is the essential guarantee of the causative performance for these miniature parts, and micromilling is the most versatile process to machine such micro-grooves. However, micromilling technology is a highly tool-dependent process, and the commercial carbide micromilling cutter has shown obvious deficiencies in terms of rapid tool wear and inferior machined quality during the machining process. In this paper, a polycrystalline diamond (PCD) micromilling cutter with a large-aspect-ratio (LAR) was designed and prepared by the self-proposed hybrid fabrication method of laser and precision grinding. Micromilling experiments on oxygen-free copper were conducted, and the carbide micromilling cutter was selected in the comparative experiments. The variations of milling forces and specific energy were analyzed through the parameter experiments. Then, the surface quality, machined accuracy and tool wear were further investigated. Results showed that the PCD micromilling cutter with an aspect ratio of 3.25 was successfully manufactured by the proposed hybrid method. The self-fabricated PCD micromilling cutter presented remarkable superiority in terms of the surface quality, machined accuracy, and tool wear when preparing deep-and-narrow micro-grooves. Finally, a satisfactory micromilled groove with an aspect ratio of 2.5 was achieved with the self-fabricated LAR PCD cutter under the optimized conditions.
Macro-Modeling for N-Type Feedback Field-Effect Transistor for Circuit Simulation
Authors: Oh Jong Hyeok , Yu Yun Seop
Published: September 2021

In this study, we propose an improved macro-model of an N-type feedback field-effect transistor (NFBFET) and compare it with a previous macro-model for circuit simulation. The macro-model of the NFBFET is configured into two parts. One is a charge integrator circuit and the other is a current generator circuit. The charge integrator circuit consisted of one N-type metal-oxide-semiconductor field-effect transistor (NMOSFET), one capacitor, and one resistor. This circuit implements the charging characteristics of NFBFET, which occur in the channel region. For the previous model, the current generator circuit consisted of one ideal switch and one resistor. The previous current generator circuit could implement IDS-VGS characteristics but could not accurately implement IDS-VDS characteristics. To solve this problem, we connected a physics-based diode model with an ideal switch in series to the current generator circuit. The parameters of the NMOSFET and diode used in this proposed model were fitted from TCAD data of the NFBFET, divided into two parts. The proposed model implements not only the IDS-VGS characteristics but also the IDS-VDS characteristics. A hybrid inverter and an integrate and fire (I&F) circuit for a spiking neural network, which consisted of NMOSFETs and an NFBFET, were simulated using the circuit simulator to verify a validation of the proposed NFBFET macro-model.
Mechanism of Spontaneous Surface Modifications on Polycrystalline Cu Due to Electric Fields
Authors: Kuppart Kristian , Vigonski Simon , Aabloo Alvo , Wang Ye , Djurabekova Flyura , Kyritsakis Andreas , Zadin Veronika
Published: September 2021

We present a credible mechanism of spontaneous field emitter formation in high electric field applications, such as Compact Linear Collider in CERN (The European Organization for Nuclear Research). Discovery of such phenomena opens new pathway to tame the highly destructive and performance limiting vacuum breakdown phenomena. Vacuum breakdowns in particle accelerators and other devices operating at high electric fields is a common problem in the operation of these devices. It has been proposed that the onset of vacuum breakdowns is associated with appearance of surface protrusions while the device is in operation under high electric field. Moreover, the breakdown tolerance of an electrode material was correlated with the type of lattice structure of the material. Although biased diffusion under field has been shown to cause growth of significantly field-enhancing tips starting from initial nm-size protrusions, the mechanisms and the dynamics of the growth of the latter have not been studied yet. In the current paper we conduct molecular dynamics simulations of nanocrystalline copper surfaces and show the possibility of protrusion growth under the stress exerted on the surface by an applied electrostatic field. We show the importance of grain boundaries on the protrusion formation and establish a linear relationship between the necessary electrostatic stress for protrusion formation and the temperature of the system. Finally, we show that the time for protrusion formation decreases with the applied electrostatic stress, we give the Arrhenius extrapolation to the case of lower fields, and we present a general discussion of the protrusion formation mechanisms in the case of polycrystalline copper surfaces.
Hybrid Cell Structure for Wideband CMUT: Design Method and Characteristic Analysis
Authors: Wang Hongliang , Huang Xiao , Yu Lijun , Ding Qi , Zhang Hanqiang , He Changde , Zhang Wendong
Published: September 2021

Capacitive micromachined ultrasonic transducer (CMUT) is an ultrasonic transducer based on the microelectromechanical system (MEMS). Wideband CMUT has good application prospects in ultrasonic imaging, ultrasonic identification, flow measurement, and nondestructive testing due to its excellent characteristics. This paper studies the method of increasing the bandwidth of the CMUT, proposes the structure of the wideband CMUT with a hybrid cell structure, and analyzes the design principles and characteristics of the wideband CMUT structure. By changing the cell spacing and the number of cells of different sizes composing the CMUT, we analyze the simulation of the effect of the spacing and number on the CMUT bandwidth, thereby optimizing the bandwidth characteristics of the CMUT. Next, the selection principle of the main structural parameters of the wideband CMUT is analyzed. According to the proposed principle, the CMUT in the air and water are designed and simulated. The results prove that both the air and water CMUT meet the design requirements. The design rules obtained in this paper can provide theoretical guidance for the selection of the main structural parameters of the wideband CMUT.
Hybrid Sparsity Model for Fast Terahertz Imaging
Authors: Ren Xiaozhen , Bai Yanwen , Jiang Yuying
Published: September 2021

In order to shorten the long-term image acquisition time of the terahertz time domain spectroscopy imaging system while ensuring the imaging quality, a hybrid sparsity model (HSM) is proposed for fast terahertz imaging in this paper, which incorporates both intrinsic sparsity prior and nonlocal self-similarity constraints in a unified statistical model. In HSM, a weighted exponentiation shift-invariant wavelet transform is introduced to enhance the sparsity of the terahertz image. Simultaneously, the nonlocal self-similarity by means of the three-dimensional sparsity in the transform domain is exploited to ensure high-quality terahertz image reconstruction. Finally, a new split Bregman-based iteration algorithm is developed to solve the terahertz imaging model more efficiently. Experiments are presented to verify the effectiveness of the proposed approach.
A Portable Device for Simple Exosome Separation from Biological Samples
Authors: Chen Wenwen , Xie Yingying , Chang Yuang , Xu Yuhai , Zhao Mengqian , Deng Pengwei , Qin Jianhua , Li Hongjing
Published: September 2021

Exosomes are membrane-bound nanovesicles secreted by most types of cells, which contain a series of biologically important molecules, such as miRNAs, proteins, and lipids, etc. Emerging evidence show that exosomes can affect the physiological status of cells and are involved in various pathological processes. However, due to their small size and density close to body fluids, it is challenging to separate exosomes from a small volume of biological samples in a simple manner. Herein, we propose a new strategy for isolating circulating exosomes from biological samples in a portable device. This method synergistically integrates chitosan electrostatic-adsorption, scaffold substrates, and shuttle flow to enable the highly effective capture of circulating exosomes with a recovery rate of over 80% within 20 min, which is much better than the performance of traditional ultracentrifugation (5–25%, 3 h). Besides, the isolated exosomes from samples could be lysed in situ and further subjected to RNA concentration detection and protein analysis. In particular, all the necessary procedures for exosome separation could be integrated into a single device without the need for bulky equipment. This established device is portable and easy to operate, which provides a promising platform for the study of exosome biology and clinical diagnosis.
Coinfection and Interference Phenomena Are the Results of Multiple Thermodynamic Competitive Interactions
Authors: Popovic Marko , Minceva Mirjana
Published: September 2021

Biological, physical and chemical interaction between one (or more) microorganisms and a host organism, causing host cell damage, represents an infection. Infection of a plant, animal or microorganism with a virus can prevent infection with another virus. This phenomenon is known as viral interference. Viral interference is shown to result from two types of interactions, one taking place at the cell surface and the other intracellularly. Various viruses use different receptors to enter the same host cell, but various strains of one virus use the same receptor. The rate of virus–receptor binding can vary between different viruses attacking the same host, allowing interference or coinfection. The outcome of the virus–virus–host competition is determined by the Gibbs energies of binding and growth of the competing viruses and host. The virus with a more negative Gibbs energy of binding to the host cell receptor will enter the host first, while the virus characterized by a more negative Gibbs energy of growth will overtake the host metabolic machine and dominate. Once in the host cell, the multiplication machinery is shared by the competing viruses. Their potential to utilize it depends on the Gibbs energy of growth. Thus, the virus with a more negative Gibbs energy of growth will dominate. Therefore, the outcome can be interference or coinfection, depending on both the attachment kinetics (susceptibility) and the intracellular multiplication machinery (permittivity). The ratios of the Gibbs energies of binding and growth of the competing viruses determine the outcome of the competition. Based on this, a predictive model of virus–virus competition is proposed.
16S rRNA Gene-Based Profiling of the Microbial Community in an Acid Mine Drainage Fe Precipitate at Libiola Mine (Liguria, Italy)
Authors: Consani Sirio , Ghignone Stefano , Pozzolini Marina , Giovine Marco , Vezzulli Luigi , Carbone Cristina
Published: September 2021

Acid mine drainage (AMD) is a common environmental problem in many sulphide mines worldwide, and it is widely accepted that the microbial community plays a major role in keeping the process of acid generation active. The aim of this work is to describe, for the first time, the microbial community thriving in goethite and jarosite Fe precipitates from the AMD of the Libiola mine. The observed association is dominated by Proteobacteria (>50%), followed by Bacteroidetes (22.75%), Actinobacteria (7.13%), Acidobacteria (5.79%), Firmicutes (2.56%), and Nitrospirae (1.88%). Primary producers seem to be limited to macroalgae, with chemiolithotrophic strains being almost absent. A phylogenetic analysis of bacterial sequences highlighted the presence of heterotrophic bacteria, including genera actively involved in the AMD Fe cycle and genera (such as Cytophaga and Flavobacterium) that are able to reduce cellulose. The Fe precipitates constitute a microaerobic and complex environment in which many ecological niches are present, as proved by the wide range of bacterial species observed. This study is the first attempt to quantitatively characterize the microbial community of the studied area and constitutes a starting point to learn more about the microorganisms thriving in the AMD of the Libiola mine, as well as their potential applications.
Substitution of Cement with Granulated Blast Furnace Slag in Cemented Paste Backfill: Evaluation of Technical and Chemical Properties
Authors: Solismaa Soili , Torppa Akseli , Kuva Jukka , Heikkilä Pasi , Hyvönen Simo , Juntunen Petri , Benzaazoua Mostafa , Kauppila Tommi
Published: September 2021

Cemented paste backfill (CPB) offers an environmentally sustainable way to utilize mine tailings, one of the largest waste streams in the world. CPB is a support and filler material used in underground mine cavities, which consists of mine tailings, water, and binder material that usually is cement. Replacing cement with secondary raw materials like granulated blast-furnace slag reduces the total CO2 emissions and strengthens the internal microstructure of the CPB. This study characterizes the total- and soluble contents of CPB starting materials and five CPB specimens containing different levels of slag substitution. In addition, phase composition (mineral liberation analysis, MLA) and internal structure (X-ray tomography) of five CPB specimens is documented, and measurements of compressive strength are used to evaluate their suitability as backfill material. Mine tailings and CPB specimens used in this study are rich in sulphates and arsenic, but low in sulphides. Stronger As leaching of ground CPB specimens compared with ground mine tailings is related to the elevating pore water pH during the cement hydration. The hydration product ettringite is found in all CPB specimens and its content is the lowest in the slag containing specimens. X-ray tomography revealed vertically differentiated density structures in the CPB specimens. The lower parts of all specimens are denser in comparison with the upper parts, which is probably due to the compaction of the solid particles at the base. The compressive strength test results indicate that partial substitution of cement with slag improves the strength of the CPB. The total replacement of cement with slag reduces the early strength but gives excellent strength and lower porosity over longer time intervals. The results of the study can be utilized in developing more durable and environmentally responsible CPB recipes for gold mines of similar mineral composition and gold extraction method.
Iron-Catalyzed Cross-Coupling Reactions of Alkyl Grignards with Aryl Chlorobenzenesulfonates
Authors: Bisz Elwira
Published: September 2021

Aryl sulfonate esters are versatile synthetic intermediates in organic chemistry as well as attractive architectures due to their bioactive properties. Herein, we report the synthesis of alkyl-substituted benzenesulfonate esters by iron-catalyzed C(sp2)–C(sp3) cross-coupling of Grignard reagents with aryl chlorides. The method operates using an environmentally benign and sustainable iron catalytic system, employing benign urea ligands. A broad range of chlorobenzenesulfonates as well as challenging alkyl organometallics containing β-hydrogens are compatible with these conditions, affording alkylated products in high to excellent yields. The study reveals that aryl sulfonate esters are the most reactive activating groups for iron-catalyzed alkylative C(sp2)–C(sp3) cross-coupling of aryl chlorides with Grignard reagents.
Falcarindiol Isolated from Notopterygium incisum Inhibits the Quorum Sensing of Pseudomonas aeruginosa
Authors: Zhao Chaoyue , Zheng Hongda , Zhou Liman , Ji Hongrui , Zhao Lu , Yu Wengong , Gong Qianhong
Published: September 2021

Quorum sensing (QS) is employed by the opportunistic pathogen Pseudomonas aeruginosa to regulate physiological behaviors and virulence. QS inhibitors (QSIs) are potential anti-virulence agents for the therapy of P. aeruginosa infection. During the screening for QSIs from Chinese herbal medicines, falcarindiol (the major constituent of Notopterygium incisum) exhibited QS inhibitory activity. The subinhibitory concentration of falcarindiol exerted significant inhibitory effects on the formation of biofilm and the production of virulence factors such as elastase, pyocyanin, and rhamnolipid. The mRNA expression of QS-related genes (lasB, phzH, rhlA, lasI, rhlI, pqsA, and rhlR) was downregulated by falcarindiol while that of lasR was not affected by falcarindiol. The transcriptional activation of the lasI promoter was inhibited by falcarindiol in the P. aeruginosa QSIS-lasI selector. Further experiments confirmed that falcarindiol inhibited the las system using the reporter strain Escherichia coli MG4/pKDT17. Electrophoretic mobility shift assay (EMSA) showed that falcarindiol inhibited the binding of the transcription factor LasR and the lasI promoter region. Molecular docking showed that falcarindiol interacted with the Tyr47 residue, leading to LasR instability. The decrease of LasR-mediated transcriptional activation was responsible for the reduction of downstream gene expression, which further inhibited virulence production. The inhibition mechanism of falcarindiol to LasR provides a theoretical basis for its medicinal application.
GSH/ROS Dual-Responsive Supramolecular Nanoparticles Based on Pillar[6]arene and Betulinic Acid Prodrug for Chemo–Chemodynamic Combination Therapy
Authors: Zhu Peng , Luo Weidan , Qian Jianqiang , Meng Chi , Shan Wenpei , Xu Zhongyuan , Zhang Wei , Liu Xin , Ling Yong
Published: September 2021

Chemodynamic therapy (CDT) based on intracellular Fenton reactions is attracting increasing interest in cancer treatment. A simple and novel method to regulate the tumor microenvironment for improved CDT with satisfactory effectiveness is urgently needed. Therefore, glutathione (GSH)/ROS (reactive oxygen species) dual-responsive supramolecular nanoparticles (GOx@BNPs) for chemo–chemodynamic combination therapy were constructed via host–guest complexation between water-soluble pillar[6]arene and the ferrocene-modified natural anticancer product betulinic acid (BA) prodrug, followed by encapsulation of glucose oxidase (GOx) in the nanoparticles. The novel supramolecular nanoparticles could be activated by the overexpressed GSH and ROS in the tumor microenvironment (TME), not only accelerating the dissociation of nanoparticles—and, thus, improving the BA recovery and release capability in tumors—but also showing the high-efficiency conversion of glucose into hydroxyl radicals (·OH) in succession through intracellular Fenton reactions. Investigation of antitumor activity and mechanisms revealed that the dramatic suppression of cancer cell growth induced by GOx@BNPs was derived from the elevation of ROS, decrease in ATP and mitochondrial transmembrane potential (MTP) and, finally, cell apoptosis. This work presents a novel method for the regulation of the tumor microenvironment for improved CDT, and the preparation of novel GSH/ROS dual-responsive supramolecular nanoparticles, which could exert significant cytotoxicity against cancer cells through the synergistic interaction of chemodynamic therapy, starvation therapy, and chemotherapy (CDT/ST/CT).
Structural Insights into Novel 15-Prostaglandin Dehydrogenase Inhibitors
Authors: Mallipeddi Prema L. , Zhang Yongyou , Li Hongyun , Markowitz Sanford D. , Posner Bruce
Published: September 2021

We discovered SW033291 in a high throughput chemical screen aimed at identifying 15-prostaglandin dehydrogenase (15-PGDH) modulators. The compound exhibited inhibitory activity in in vitro biochemical and cell-based assays of 15-PGDH activity. We subsequently demonstrated that this compound, and several analogs thereof, are effective in in vivo mouse models of bone marrow transplant, colitis, and liver regeneration, where increased levels of PGE2 positively potentiate tissue regeneration. To better understand the binding of SW033291, we carried out docking studies for both the substrate, PGE2, and an inhibitor, SW033291, to 15-PGDH. Our models suggest similarities in the ways that PGE2 and SW033291 interact with key residues in the 15-PGDH-NAD+ complex. We carried out molecular dynamics simulations (MD) of SW033291 bound to this complex, in order to understand the dynamics of the binding interactions for this compound. The butyl side chain (including the sulfoxide) of SW033291 participates in crucial binding interactions that are similar to those observed for the C15-OH and the C16-C20 alkyl chain of PGE2. In addition, interactions with residues Ser138, Tyr151, and Gln148 play key roles in orienting and stabilizing SW033291 in the binding site and lead to enantioselectivity for the R-enantiomer. Finally, we compare the binding mode of (R)-S(O)-SW033291 with the binding interactions of published 15-PGDH inhibitors.
Advances in the Synthesis of Crystalline Metallosilicate Zeolites via Interlayer Expansion
Authors: Bian Chaoqun , Yang Yichang , Luo Xiaohui , Zhang Wenxia , Zhang Jie , Zhu Longfeng , Qiu Jianping
Published: September 2021

Given the numerous industrial applications of zeolites as adsorbents, catalysts, and ion-exchangers, the development of new zeolite structures is highly desired to expand their practical applications. Currently, a general route to develop new zeolite structures is to use interlayer expansion agents to connect layered silicates. In this review, we briefly summarize the novel zeolite structures constructed from the lamellar precursor zeolites MWW, RUB-36, PREFER, Nu-6(1), COK-5, and PLS-1 via interlayer expansion. The contents of the summary contain detailed experiments, physicochemical characterizations, possible expansion mechanisms, and catalytic properties. In addition, the insertion of metal heteroatoms (such as Ti, Fe, Sn) into the layered zeolite precursor through interlayer expansion, which could be helpful to modify the catalytic function, is discussed.