bluuebunny/crossref_metadata_2025 huggingface dataset:
Window Opening or Mechanical Ventilation Systems for Italian Schools? Energetic Aspects, CO2 Concentration and Infection Risk Assessment Based on the SARS-CoV-2 Data
Authors: Busato Filippo , Cavallini Alberto
Published: July 2023

During the first COVID outbreak, schools were seen as a substantial issue regarding the spread of the disease, since schools are the most densely occupied indoor environment continuously over time. The infection risk of SARS-CoV-2 could reach large levels, especially in locations with inadequate ventilation rates, according to the current knowledge of airborne transmitted diseases. The first main drive to reduce viral concentration is dilution, provided by infiltration, air change through aeration (window opening), mechanical ventilation; the second drive is filtration. After a brief investigation of the prescriptions and requirements for ventilation in school rooms stipulated by Italian law, the present work compares the energy requirements for ventilation in two main cases—window opening (and infiltration) and mechanical ventilation—the effect of wearing or not wearing a mask is also considered. Afterward, the focus is shifted to the infection risk assessment, comparing the two different ventilation techniques as mentioned above. Not only is it found that window opening is far from an effective technique for controlling the spread of the disease; it is also found that for the same amount of thermal energy needed, mechanical ventilation can reduce the individual infection risk by a factor of 3, thus providing a better IAQ. It is also proved that mechanical ventilation, in terms of infection control, can not only replace but even outperform the effect of masks.
Experimental Study on Bending Performance of High-Performance Fiber-Reinforced Cement Composite Prefabricated Monolithic Composite Beams
Authors: Lu Tingting , Guan Kai , Jin Haowei
Published: July 2023

To enhance the mechanical properties and damage resistance of prefabricated monolithic composite beams, this study introduces HPFRCC precast mold shells as a replacement for ordinary concrete in the construction of prefabricated monolithic composite beams. These HPFRCC precast mold shell prefabricated monolithic composite beam members are then subjected to experimental investigations to analyze their flexural properties. The results of the study indicate that the U–shaped HPFRCC precast mold shell exhibits excellent bonding with the post-cast concrete, with no significant peeling observed. Moreover, compared to ordinary cast-in-place monolithic RC beams, the HPFRCC/RC prefabricated monolithic composite beams demonstrate a 17.2% increase in peak load and a 24.55% increase in yield load. Similarly, the HPFRCC/RC prefabricated monolithic composite beams show an 8.1% increase in peak load and a 5.59% increase in yield load compared to ordinary RC composite beams. In comparison to both ordinary cast-in-place monolithic RC beams and ordinary RC composite beams, the cracks observed in the HPFRCC/RC prefabricated monolithic composite beams are denser and finer, with a smaller crack development rate and width. These findings suggest that the incorporation of HPFRCC materials improves the damage resistance of the beam members.
Characteristics of Internal Water Flow Conduction within Asphalt Mixtures Based on Real Three-Dimensional Void Structure
Authors: Wan Cheng , Yi Qiang , Zhang Xiaoning
Published: July 2023

This work presents a new approach to investigating water conduction properties in real three-dimensional (3D) voids of asphalt mixtures. Three different molding methods were employed for the same grade of asphalt mixture, and the three asphalt mixture specimens were scanned using X-ray Computerized Tomography (CT) to identify the real 3D void structure distribution inside the mixture. The real 3D behavior of void moisture conduction inside the mixture was simulated using the discrete lattice Boltzmann method and the BGK collision model. Three different molding methods were used to study the behavior of mesoscopic seepage inside the specimen. The results show that water conduction varies substantially in real 3D voids inside diverse molded objects. Regardless of flow and flow velocity, the Superpave Gyratory Compactor (SGC) method is extraordinarily close to the conduction qualities of the actual field core material. It shows that the Marshall molding method is inconsistent with the actual pavement molding method, and the SGC method can not only ensure that the reasonable void ratio is conducive to the thermal expansion and cold shrinkage space of the asphalt mixture but also prevents rainwater from entering the asphalt mixture. This work provides a new perspective for the study of water damage resistance and medium transmission characteristics of asphalt mixtures.
Bio-Induced Healing of Cement Mortars in Demineralized and Danube Water: CNN Model for Image Classification
Authors: Nešković Jasmina , Jovanović Ivana , Markov Siniša , Vučetić Snežana , Ranogajec Jonjaua , Trumić Milan
Published: July 2023

Reducing the costs of repairing concrete structures damaged due to the appearance of cracks and reducing the number of people involved in the process of their repair is the subject of a multitude of experimental studies. Special emphasis should be placed on research involving industrial by-products, the disposal of which has a negative environmental impact, as is the case in the research presented in this paper. The basic idea was to prepare a mortar with added granulated blast furnace slag from Smederevo Steel Mill and then treat artificially produced cracks with a Sporosarcina pasteurii DSM 33 suspension under the conditions of both sterile demineralized water and water from the Danube river in order to simulate natural conditions. The results show a bio-stimulated healing efficiency of 32.02% in sterile demineralized water and 42.74% in Danube river water already after 14 days. The SEM images clearly show calcium carbonate crystals as the main compound that has started to fill the crack, and the crystals are much more developed under the Danube river water conditions. As a special type of research, microscopic images of cracks were classified into those with and without the presence of bacterial culture. By applying convolutional neural networks (ResNet 50), the classification success rate was 91.55%.
Developing a Model for Analyzing Risks Affecting Machinery Tunnel Execution
Authors: Eid Mohamed A. , Hu Jong Wan , Issa Usama
Published: July 2023

Tunneling projects face several risks during the execution stage that affect the execution objectives (cost, time, quality, and safety). This study aimed to define the main execution activities of machinery tunnels with the associated risk factors and to develop a model for evaluating and analyzing the effects of the risk factors on the execution stage. The recognized activities of executing tunnels included the following: (A01) thrust and reception shaft installation; (A02) machine setup and break-in; (A03) machine progression and lining placing; and (A04) machine break-out and removal. Additionally, thirty-two risk factors associated with these activities were identified. Risk factor probability of occurrence and impacts on cost, time, quality, and safety were determined. Due to this risky and uncertain environment, the fuzzy logic method was applied for developing a model to analyze the effects of the risks on the tunneling process. The model was applied and verified using data collected in Egypt. Many correlations were determined among risk factors that affected tunneling execution objectives, resulting in close relationships with each other. The results emphasized many significant risk factors, such as “conflict between technical geological report and the ground nature”, and “shaft wall damage during break-out”. A03, which is related to machine progression and lining placing, was declared the riskiest activity group during tunneling execution. Further, safety was rated as the objective most affected by risks. The risk model presented in this study can be modified and applied to other cases, while the results and key risks can support the decision-makers who deal with tunneling construction.
Study on the Preparation Method of Quality-Assured In-Hospital Drug Formulation for Children—A Multi-Institutional Collaborative Study
Authors: Saito Jumpei , Suzuki Eiji , Nakamura Yosuke , Otsuji Takashi , Yamamoto Hiroshi , Yamamoto Hideki , Kai Yuiko , Totsu Maiko , Hashimoto Sayuki , Nakamura Hidefumi , Akabane Miki , Yamatani Akimasa
Published: July 2023

The quality-assured preparation of crushed and diluted preparations for children is a challenge. In this study, a multicenter study was conducted to validate the preparation method for the quality assurance of baclofen powder, clonidine powder, and hydrocortisone powder prepared from tablets according to a previously established method. In-hospital preparations were prepared at five medical facilities under different crushing and mixing conditions. After storage in closed bottles, in-use bottles, and laminated paper for 120 days, ingredients stability, drug elution, and content uniformity after packaging were evaluated. All three ingredients were maintained at between 90% and 110% of their initial content for 120 days under packaging conditions of 25 ± 2 °C and 60 ± 5% relative humidity, with no change in dissolution in all formulations made at all five facilities. The content uniformity was also acceptable. The established method may contribute to quality-assured pediatric dosage form modification.
Overcoming Bureaucratic Resistance: An Analysis of Barriers to Climate Change Adaptation in South Africa
Authors: Sibiya Nomfundo Patricia , Das Dillip Kumar , Vogel Coleen , Mazinyo Sonwabo Perez , Zhou Leocadia , Kalumba Mukalazi Ahmed , Sithole Mikateko , Adom Richard Kwame , Simatele Mulala Danny
Published: July 2023

Climate change is already a reality, and it is affecting the lives and livelihoods of many people globally. Many scientists argue that adaptation is, therefore, necessary to address the impact of climate change on life-supporting systems. Climate change adaptation, however, is a complex process that involves transformations implemented through governance at multiple levels. In this paper, the barriers to climate change adaptation in South Africa are presented and analysed. Semi-structured, in-depth interviews were conducted telephonically and online via Microsoft Teams with 13 government officials working at the Department of Forestry, Fisheries, and the Environment; the KwaZulu-Natal Department of Economic Development and Environmental Affairs; and the uMkhanyakude District Municipality. The findings suggest that the barriers to climate change adaptation in South Africa include inadequate financial resources, a lack of human capacity at the provincial and local levels, limited political will at the local level, limited understanding of climate change adaptation issues by communities, inadequate coordination across government levels and sectors, no legal mandate at the local level, no climate change unit at the district and local levels, a lack of knowledge by some staff members tasked with environmental duties at the local level, not enough climate change plans in place at the local level, and outdated information on climate change used in the IDPs. This paper, therefore, recommends that climate change be a standing item in the Integrated Development Plan for local governments, which will ensure that climate change is budgeted for appropriately. In addition, this paper suggests that a mandate for climate change adaptation be developed for all three government levels. There is also a need for the government to invest in capacity development and improve horizontal and vertical coordination to strengthen the weak climate governance capacity that exists.
A Theoretical Review of Socio-Economic Dynamics and Structural Transformation of Society: Traits and Constraints in Validating Response Processes Due to Unpredictable Factors
Authors: Vasin Sergey Mikhailovich
Published: July 2023

To investigate and create theories of socio-economic dynamics has always been relevant for professionals in theoretical and applied economics. These theories are urgent in periods of global and catastrophic events since, on the one hand, they can explain the emergence of critical structural changes in society, and on the other hand, they can determine the likelihood of surviving of crisis processes. However, there are some drawbacks involved therein, and the poor ability to predict unexpected and shocking events, such as the COVID-19 pandemic, is a key one. The presented review outlines transformation patterns of socio-economic systems stemming from diverse contradictions. Some widely known transformation theories of socio-economic systems are presented. The major traits of such theories and specific approaches to explaining the features of the emergence and development of dynamic processes are demonstrated. The feasibility of practical application, as well as constraints in explaining and predicting the transformation of society, is indicated. Some theories that consider the transformation of society as a distinct process are specified, and approaches that characterize major transformations as elements of socio-economic dynamics are defined.
The Role of Play and Objects in Children’s Deep-Level Learning in Early Childhood Education
Authors: Sando Ole Johan , Sandseter Ellen Beate Hansen , Brussoni Mariana
Published: July 2023

This research investigates the significance of the physical environment in early childhood education and care (ECEC) institutions as a facilitator of deep-level learning. Building upon Laevers’ concept of deep-level learning, this study explores the interplay between objects in ECEC settings, children’s play, and their deep-level learning. The primary objective is to examine the potential mediating role of play in the relationship between objects and deep-level learning. The research methodology involves the analysis of a sample consisting of 928 two-minute video observations collected from eight ECEC institutions in Norway. The results demonstrate a positive association between children’s engagement in play, their utilization of objects, and deep-level learning. The findings suggest that constructive and symbolic play partly mediate the positive relationship between deep-level learning and object utilization. These outcomes highlight the pivotal role of play in early childhood education and emphasize how elements within the physical environment can effectively support children’s learning.
Memristor-Based D-Flip-Flop Design and Application in Built-In Self-Test
Authors: Dai Guangzhen , Xie Wenxin , Du Xingyan , Han Mingjun , Ni Tianming , Wu Daohua
Published: July 2023

There are several significant advantages of memristors, such as their nano scale, fast switching speed, power efficiency and compatibility with CMOS technology, as one of the alternatives in the next generation of semiconductor storage devices. D-flip-flops (DFFs) based on the traditional CMOS process have some shortcomings, including a large area, high power, and charge leakage when scaling down. However, memristors offer a new approach to the design of DFFs with improved performance. Two simplified edge-triggered DFFs are proposed to reduce the number of devices via the Memristor-Rationed Logic (MRL) method, which utilizes the characteristic of transmitting signals in the two-stage inversion structure. In addition, two new 4-bit Linear Feedback Shift Registers (LFSRs) are designed and verified using the proposed DFFs. Compared to the partially existing LFSRs, the designed LFSRs reduce the number of devices significantly, decrease the power consumption by 32.7% and 33.3% and shorten the delay time by 34.5% and 30.7% for the NOR and NAND gates, respectively. Finally, the proposed falling-edge-triggered DFF is used to implement the major blocks of the Built-In Self-Test(BIST) circuit, and the simulation results confirm their correctness and feasibility.
Packet Reordering in the Era of 6G: Techniques, Challenges, and Applications
Authors: Lin Jiaqi , Zhang Xiaofeng , Gao Xianming , Kang Pengtao , Zhou Yuxi , Ouyang Ying , Feng Tao
Published: July 2023

The advent of sixth-generation (6G) networks brings unmatched speed, reliability, and capacity for massive connections, making it a cornerstone for revolutionary applications. One such application is in vehicular networks, which have their unique demands and complexities. Specifically, they face the complex issue of packet reordering due to the high-speed movement of vehicles and frequent switching of network connections. This paper examines the impact and causes of packet reordering, its threats to network efficiency, and potential countermeasures, particularly in the context of 6G-enabled vehicular networks. We introduce end-to-end methods and metrics to address packet reordering in 6G, discussing the development trends and application prospects. Our findings highlight the emergence of sophisticated strategies, such as prediction and avoidance, to manage packet reordering. They also reveal potential applications to boost network reliability, emulate traffic distributions, and enhance data security. Furthermore, we anticipate a growing integration of machine learning and data-driven optimization in tackling packet reordering. The insights provided aim to influence the future design and optimization of 6G networks, particularly concerning packet management and performance. This paper aims to assist researchers and practitioners in effectively leveraging packet reordering to promote efficient and secure operations of future 6G networks.
A DRL-Based Load Shedding Strategy Considering Communication Delay for Mitigating Power Grid Cascading Failure
Authors: Wei Yongjing , Tian Anqi , Jiang Yingjie , Zhang Wenjian , Ma Liqiang , Ma Liang , Sun Chao , Sun Jian
Published: July 2023

Successive failures in power transmission lines can cause cascading failures in the power grid, which may eventually affect large parts of the power grid and even cause the power grid system to go down. Collecting and transmitting primary equipment information and issuing load-shedding action commands in the power grid depend on the power communication network. With the help of the power communication network, we can better observe the situation of the power grid in real time and provide a guarantee for the regular working of the power grid. However, the communication network also has the problem of communication delay causing latency in load-shedding action. On the premise of preserving the key physical properties and operational characteristics of the power grid, this paper uses the IEEE 14 and 30 bus systems as examples to establish a direct current (DC) power flow simulation environment. We establish a communication network model based on the power grid topology and the corresponding communication channels. For the problem of cascading failures occurring in the power grid after transmission line failures, a load-shedding strategy using soft actor-critic (SAC) based on deep reinforcement learning (DRL) was developed to effectively mitigate cascading failures in the power grid while considering the impact of communication delay. The corresponding communication delay is obtained by calculating the shortest communication path using the Dijkstra algorithm. The simulation verifies the feasibility and effectiveness of the SAC algorithm to mitigate cascading failures. The trained network can decide on actions and give commands quickly when a specific initial failure is encountered, reducing the scale of cascading failures.
Wavelet Threshold Ultrasound Echo Signal Denoising Algorithm Based on CEEMDAN
Authors: Li Zhiwei , Xu Huyue , Jiang Bibo , Han Fangfang
Published: July 2023

In this study, an algorithm for denoising ultrasound echo signals in industrial settings is proposed to address the problem of high noise and low signal-to-noise ratio. The algorithm combines complete ensemble empirical mode decomposition with adaptive noise (CEEMDAN), mutual information entropy (MIE), and wavelet threshold denoising to ensure effectiveness given the unique structure of ultrasound echo signals. Initially, CEEMDAN is used to decompose the signal into intrinsic mode function (IMFs) and residual signals. The MIE is then used to determine the correlation of neighboring IMF signals, which are then divided into a noise- and a signal-dominated part. Finally, using wavelet thresholding, noise is suppressed in the signal-dominant part, and the resulting denoised signal is reconstructed using the residual signal. The performance of the algorithm is verified through simulations and physical experiments, and the results show that it is superior to traditional signal denoising methods.
Multi-Beam Luneburg Lens with Reduced Size Patch Antenna
Authors: Muhamad Nadzir Norsaidah , Himdi Mohamed , A. Rahim Mohamad Kamal , Murad Noor Asniza , Ayop Osman , Lafond Olivier
Published: July 2023

This paper describes the integration of a plate Luneburg lens with a microstrip patch antenna (MPA) with a complementary split ring resonator (CSRR) at 17 GHz frequency. The main advantage of the method is the compact size of the optimized MPA with CSRR such that the radiation pattern of the antennas successfully overlaps at −3 dB. The overlapping is achieved by positioning multiple MPA with CSRR structures around the plate Luneburg lens with 0.408λ distance between the elements. To test the performance of the lens, CST simulations are carried out using a classical WR62 open-ended waveguide to feed the structure, then adding more waveguides rotated at focal points of the lens. After that, the waveguide is substituted with the MPA with CSRR structure. The superiority of the proposed method over the conventional waveguide or regular patch antenna is confirmed by an overlap in radiation pattern at −3 dB with a narrow beamwidth of 10.9°.
Frequency Support Control of Multi-Terminal Direct Current System Integrated Offshore Wind Farms Considering Direct Current Side Stability
Authors: Han Huachun , Li Qun , Li Qiang
Published: July 2023

The frequency stability of modern power systems is challenged due to widespread application of large-scale renewable energy systems, of which the offshore wind farm (OWF) is one of the dominant resources. The OWFs are usually integrated into the grid by multi-terminal direct current (MTDC) transmission systems, which makes the energy flow complicated and the frequency control design challenging. A frequency support control method of MTDC system integrated OWFs (referred to as the OWF-MTDC system) is proposed in this paper. First, the wind turbine generation system (WTGS) is controlled to reserve a certain amount of available power according to the real-time wind speed for more comprehensive frequency regulation. Then, the frequency support control of OWFs is designed, and they can release the rotor kinetic energy and reserved power to support the onshore grid frequency. In addition, the virtual inertia control of a modular multi-level converter (MMC) is designed, which can also provide frequency support in an emergency by use of the DC capacitor. To ensure that the frequency control of the OWF-MTDC system does not degrade the stability of the system, a detailed DC impedance model of the MMC-based MTDC systems is developed, considering the constant power control and DC voltage control. Based on the impedance model, the impact of the frequency control coefficients on the DC side stability of the MTDC system is analyzed. Simulation results validate the stability analysis and verify the proposed frequency control method, which can effectively provide frequency support to the onshore power grid.
The Modeling of Fuel Auto-Ignition Delay and Its Verification Using Diesel Engines Fueled with Oils with Standard or Increased Cetane Numbers
Authors: Cisek Jerzy , Leśniak Szymon
Published: July 2023

This article contains the results of mathematical modeling of the self-ignition delay (τc sum) of a single droplet for various fuels, and the results of measurement verification (τc) of this modeling in diesel engines. The result of modeling the τc sum (as a function of the diameter and ambient temperature of the fuel droplet) revealed two physical and two chemical stages that had different values of the weighting factor (WFi) in relation to the total delay of self-ignition. It was also found that the WFi values of individual phases of the self-ignition delay differed for different fuels (conventional and alternative), and in the total value of τc sum. The measured value of the self-ignition delay (τc) was determined in tests using two diesel engines (older—up to EURO II and newer generation—from EURO IV). The percentage difference in the Δτc sum value obtained from modeling two fuels with different cetane number values was compared with the percentage difference in the Δτc value for the same fuels obtained during the engine measurements. Based on this analysis, it was found that the applied calculation model of the self-ignition delay for a single fuel droplet can be used for a comparative analysis of the suitability of different fuels in the real conditions of the cylinder of a diesel engine. This publication relates to the field of mechanical engineering.
Hi-Accuracy Method for Spectrum Shift Determination
Authors: Pavlycheva Nadezhda , Niyazgulyyewa Ayna , Sakhabutdinov Airat , Anfinogentov Vladimir , Morozov Oleg , Agliullin Timur , Valeev Bulat
Published: July 2023

A new hi-accuracy method for slight-shift determination of low-resolution spectra is proposed. The method allows determining a spectrum shift with an accuracy exceeding the spectrum analyzer resolution to more than three orders of magnitude due to the mathematical post-processing. The method is based on representing the spectrum as a continuous and everywhere differentiable function; expanding it into the Taylor series; approximating all the function derivatives by finite differences of a given order. Thereafter, the spectrum shift is determined using the least-squares method. The method description, its mathematical foundation and the simulation results are given. The advantages of the application of the proposed method are shown.
Improvement in the Stability and Bioaccessibility of Carotenoid and Carotenoid Esters from a Papaya By-Product Using O/W Emulsions
Authors: Lara-Abia Sara , Lobo Gloria , Pérez-Pascual Noelia , Welti-Chanes Jorge , Cano M. Pilar
Published: July 2023

The aim of the present work was to improve the stability and bioaccessibility of carotenoids from green oil extracts obtained from papaya by-products using oil-in-water (O/W) emulsions. The effects of different concentrations of pectin (1%, 2%, and 3%), a high-molecular-size emulsifier, together with Tween 20, a low-molecular-size emulsifier, high-speed homogenization conditions (time: 2, 3, 4, and 5 min; rpm: 9500, 12,000, 14,000, and 16,000 rpm), and high-pressure homogenization (HPH) (100 MPa for five cycles) were evaluated to determine the optimal conditions for obtaining O/W stable emulsions with encapsulated carotenoids. Soybean, sunflower, and coconut oils were used to formulate these O/W emulsions. The bioaccessibility of the main individual encapsulated papaya carotenoids was evaluated using the INFOGEST digestion methodology. In addition, the microstructures (confocal and optical microscopy) of the O/W carotenoid emulsions and their behavior during in vitro digestion phases were studied. Sunflower O/W carotenoid emulsions showed smaller mean particle size, higher negative ζ-potential, and higher viscosity than soybean O/W emulsions. Particle size reduction in the O/W emulsions using the HPH process improved the bioaccessibility of papaya encapsulated carotenoids. In these O/W emulsions, depending on the vegetable oil, lycopene was the carotenoid with the highest bioaccessibility (71–64%), followed by (all-E)-β-carotene (18%), (all-E)-β-cryptoxanthin (15%), and (all-E)-β-cryptoxanthin laurate (7–4%). These results highlight the potential of using green carotenoid papaya extracts to formulate O/W emulsions to enhance carotenoid bioactivity by efficiently preventing degradation and increasing in vitro bioaccessibility.
Metabolomics Reveals Distinctive Metabolic Profiles and Marker Compounds of Camellia (Camellia sinensis L.) Bee Pollen
Authors: Qi Dandan , Lu Meiling , Li Jianke , Ma Chuan
Published: July 2023

Camellia bee pollen (CBP) is a major kind of bee product which is collected by honeybees from tea tree (Camellia sinensis L.) flowers and agglutinated into pellets via oral secretion. Due to its special healthcare value, the authenticity of its botanical origin is of great interest. This study aimed at distinguishing CBP from other bee pollen, including rose, apricot, lotus, rape, and wuweizi bee pollen, based on a non-targeted metabolomics approach using ultra-high performance liquid chromatography–mass spectrometry. Among the bee pollen groups, 54 differential compounds were identified, including flavonol glycosides and flavone glycosides, catechins, amino acids, and organic acids. A clear separation between CBP and all other samples was observed in the score plots of the principal component analysis, indicating distinctive metabolic profiles of CBP. Notably, L-theanine (864.83–2204.26 mg/kg) and epicatechin gallate (94.08–401.82 mg/kg) were identified exclusively in all CBP and were proposed as marker compounds of CBP. Our study unravels the distinctive metabolic profiles of CBP and provides specific and quantified metabolite indicators for the assessment of authentic CBP.
Microbial Community Dynamics and the Correlation between Specific Bacterial Strains and Higher Alcohols Production in Tartary Buckwheat Huangjiu Fermentation
Authors: Yin Sheng , Huang Mingquan , Wang Jiaxuan , Liu Bo , Ren Qing
Published: July 2023

Tartary buckwheat is a healthy grain rich in nutrients and medicinal ingredients and consequently is commonly used for Huangjiu brewing. In order to reveal the correlation between microbial succession and higher alcohols production, in this study, Huangjiu fermentation was conducted using Tartary buckwheat as the raw material and wheat Qu as the starter culture. Microbial community dynamics analysis indicated that the bacterial diversity initially decreased rapidly to a lower level and then increased and maintained at a higher level during fermentation. Lactococcus was the dominant bacteria and Ralstonia, Acinetobacter, Cyanobacteria, and Oxalobacteraceae were the bacterial genera with higher abundances. In sharp contrast, only 13 fungal genera were detected during fermentation, and Saccharomyces showed the dominant abundance. Moreover, 18 higher alcohol compounds were detected by GC-MS during fermentation. Four compounds (2-phenylethanol, isopentanol, 1-hexadecanol, and 2-phenoxyethanol) were stably detected with high concentrations during fermentation. The compound 2-ethyl-2-methyl-tridecanol was detected to be of the highest concentration in the later period of fermentation. Correlation analysis revealed that the generation of 2-phenylethanol, isopentanol, 1-hexadecanol, and 2-phenoxyethanol were positively correlated with Granulicatella and Pelomonas, Bacteroides, Pseudonocardia and Pedomicrobium, and Corynebacterium, respectively. The verification fermentation experiments indicated that the improved wheat Qu QT3 and QT4 inoculated with Granulicatella T3 and Acidothermus T4 led to significant increases in the contents of 2-phenylethanol and pentanol, as well as isobutanol and isopentanol, respectively, in the Tartary buckwheat Huangjiu. The findings benefit understanding of higher alcohols production and flavor formation mechanisms in Huangjiu fermentation.
Corn Steep Liquor Application Improves Pepper (Capsicum annum L.) Tolerance to Salinity
Authors: Navarro-Morillo Iván , Pardo-Pina Sofía , Garcia-Sánchez Francisco , Ruiz Juan , Laserna-Arcas Santiago , Plasencia Felix , Cámara-Zapata José
Published: July 2023

Salinity, caused by irrigation with water containing high salt concentrations, excessive fertilization, or the loss of leaching capacity in some soils, is a serious problem on a global scale. Its incidence leads to osmotic and specific effects, as well as an imbalance in nutrient uptake that hinders the growth of most crops. Biostimulants can improve salt tolerance by reducing the uptake and accumulation of toxic ions. Corn steep liquor (CSL) is a byproduct of corn cleaning and maceration. This study investigates whether CSL application induces adaptive responses in pepper (Capsicum annuum L.) plants cultivated under saline conditions. Four treatments were carried out with pepper plants in a culture chamber: irrigation with Hoagland nutrient solution; irrigation with 100 mM NaCl in the Hoagland nutrient solution; irrigation with 100 mM NaCl in the Hoagland nutrient solution and the foliar application of CSL at 5 mL L−1 every 7 days; and irrigation with 100 mM NaCl in the Hoagland nutrient solution and root application of CSL at 5 mL L−1 every 7 days. The beneficial effect of CSL in reducing the phytotoxicity of salt stress was found to be due to an improvement in the photosynthetic efficiency and a reduction in the generation of reactive oxygen species. Thus, the increase in MDA concentration due to saline treatment is less when applying CSL, which is 3.5 times less when it is performed via the foliar route and 4.6 times if the treatment is on the root. The results show that CSL application increased the aerial biomass and leaf area under saline conditions through physiological mechanisms that varied depending on the application method.
Bruxism and Botulinum Injection: Challenges and Insights
Authors: Malcangi Giuseppina , Patano Assunta , Pezzolla Carmela , Riccaldo Lilla , Mancini Antonio , Di Pede Chiara , Inchingolo Alessio Danilo , Inchingolo Francesco , Bordea Ioana Roxana , Dipalma Gianna , Inchingolo Angelo Michele
Published: July 2023

Botulinum toxin (BTA) is a bacterial-derived extract that can inhibit muscle contraction, acting directly on the absorption of acetylcholine. Thanks to this property, botulinum has been used in aesthetic and general medicine for several years. Nowadays, the use of botulinum toxin is being deepened to address the problem of bruxism. In this scoping review, the results of the studies in the literature of the last 10 years were analyzed. Indeed, 12 reports (found on PubMed, Web of Science, and Scopus, entering the keywords “BRUXISM” and “BOTULINUM TOXIN”) were deemed eligible for inclusion in this review. In the studies reviewed, BTA was injected into different muscle groups: masseters, masseter and temporalis or masseter, temporalis, and medial pterygoid. Botulinum toxin injection is a viable therapeutic solution, especially in patients with poor compliance or without improvement in conventional treatment.
Meek Micro-Skin Grafting and Acellular Dermal Matrix in Pediatric Patients: A Novel Approach to Massive Extravasation Injury
Authors: Maruccia Michele , Tedeschi Pasquale , Corrao Claudia , Elia Rossella , La Padula Simone , Di Summa Pietro G. , Maggio Giulio M. M. , Giudice Giuseppe
Published: July 2023

(1) Background: Extravasation injuries in pediatric patients can lead to significant harm if they are not promptly diagnosed and treated. However, evidence-based standardization on extravasation management remains limited, particularly for extensive wound necrosis. This case report presents the management of an 8-week-old premature patient with an extensive extravasation injury involving the right forearm and dorsum of the hand. (2) Methods: The patient was evaluated by a multidisciplinary team in our Neonatal Intensive Care Unit. Surgical intervention involved the debridement of necrotic tissues, followed by temporary coverage with an acellular dermal matrix. Definitive coverage was achieved through Meek micrografting after three weeks. Physical therapy was provided with pre- and post-rehabilitation range of motion assessed using goniometric measurements. Scar quality was evaluated using the Vancouver Scar Scale. (3) Results: The engraftment rate of the Meek micrografts was 93%, with 16 out of 226 micrografts lost. The patient achieved a Vancouver Scar Scale score of 6, indicating a moderate degree of scarring. Significant improvements in elbow, wrist, and finger joint range of motion were observed at a 1-year follow-up. (4) Conclusions: Close observation and heightened awareness of extravasation risks by trained personnel are crucial. Meek micrografting combined with dermal substitute coverage represents an innovative approach to managing extravasation wounds in pediatric patients.
Surgery and Prophylaxis with Susoctocog-Alfa in Acquired Hemophilia: Case Series and Literature Review
Authors: Sella Carola , Bardetta Marco , Valeri Federica , Dainese Cristina , Valpreda Alessandra , Massaia Massimo , Grimaldi Daniele , Porreca Annamaria , Bruno Benedetto , Borchiellini Alessandra
Published: July 2023

Background: Acquired hemophilia A (AHA) is a rare bleeding disease due to autoantibodies directed against clotting factor VIII (FVIII). Treatment of AHA consists of inhibitor eradication with immunosuppressive therapy (IST) and prompt control of bleeding obtained with bypassing agents or recombinant porcine FVIII (rpFVIII). The latter has recently been licensed for management of acute bleeding in AHA. Unlike treatment with bypassing agents, rpFVIII can be monitored to provide a successful hemostatic effect and avoid overtreatment. Correlation between rpFVIII inhibitor titers and efficacy of rpFVIII treatment remains a matter of debate. Methods: We report three cases of AHA in which rpFVIII was successfully used with an unconventional schedule despite the presence of medium–high titers of the rpFVIII. The modified Nijmegen–Bethesda inhibitor assay (NBA) was used to dose porcine FVIII inhibitors. Result: The presence of rpFVIII inhibitors prior to the exposition to susoctocog-alfa, that may suggest a cross-reactivity with human FVIII inhibitors, did not affect hemostasis. Conclusion: In our experience, rpFVIII demonstrates safety and efficacy in the presence of rpFVIII inhibitors and using an unconventional schedule in both the perioperative and outpatient settings. Laboratory measurement of inhibitors against rpFVIII during treatment is described for the first time.
The Impact of Xerostomia on Food Choices—A Review with Clinical Recommendations
Authors: Müller Frauke , Chebib Najla , Maniewicz Sabrina , Genton Laurence
Published: July 2023

Xerostomia and hyposalivation are highly prevalent conditions in old age, particularly among multimorbid elders, and are often attributed to the use of multiple medications. These conditions negatively affect oral functions, such as chewing, swallowing, speech, and taste. Additionally, the lack of lubrication of the oral mucosa frequently leads to super-infections with candida. Denture retention and comfort may also be compromised. The risk of dental caries and erosion of natural teeth increases since saliva, which is essential for repairing initial lesions in tooth structures, is insufficient. The dry sensation in the mouth also impacts the emotional and social well-being of elderly individuals. Patients experiencing xerostomia often avoid certain foods that are uncomfortable or difficult to consume. However, some foods may alleviate the symptoms or even stimulate salivation. This review discusses the limited available evidence on nutritional advice for patients with xerostomia and aims to provide insight into the patient’s perspective while offering clinical recommendations. Future studies should focus on investigating the nutritional intake of individuals suffering from xerostomia or hyposalivation in order to ensure oral health comfort, prevent malnutrition, and minimize the impact on their quality of life.
Respiratory Muscle Function and Exercise Performance in Adult Patients with Fontan Circulation
Authors: Ladrón-Abia Raquel , Cejudo Ramos Pilar , Valverde Pérez Israel , Camacho Fernández De Liger Rocío , Wals-Rodríguez Amadeo-José , Rodríguez Puras María José , Manso García Begoña , Gallego Pastora
Published: July 2023

At rest, a good Fontan circulation can provide a normal cardiac output (CO). However, as a consequence of its unique hemodynamic nature, the limitations of the Fontan circuit are exposed during exercise. We aimed to provide a comprehensive assessment of the pathophysiology of exercise in adult Fontan patients (FPs) and identify factors limiting their functional capacity (FC). In a single-center study conducted in 37 FPs aged ≥16 years and 19 healthy-controls (HCs) who underwent CPET on a cycle ergometer in February and March 2022, the mean peakVO2 was 21 ± 5.4 mL/kg/min, which was 55% of the predicted value. Morphologically, the left single ventricle showed a higher peakVO2% predicted value (57.4 ± 14.4% vs. 43.4 ± 8.1%, p = 0.045). The factors associated with low peakVO2 values were an early flattened or descending O2 pulse at maximal exertion (52 ± 14% vs. 62 ± 12.5, p = 0.04 and 47.6 ± 9% vs. 60 ± 14, p = 0.018, respectively) and chronotropic insufficiency (53 ± 12% vs. 69.8 ± 20%, p = 0.008). The OUES was found to be a useful parameter to assess the FC in FPs in maximal and submaximal exercise testing. A strong positive correlation was observed between the %OUES and peakVO2%predicted (r = 0.726, p > 0.001). The lung function was impaired in the FPs, mostly with a mild restrictive pattern (56.8%). The FPs showed lower inspiratory muscle strength compared to the HCs but it was not statistically associated with either the peakVO2 or VE/VCO2 slope. Regular intense physical activity improves one’s FC. Although FPs have inspiratory muscle weakness, its impact on their FC is unclear. The peakVO2% predicted grew progressively higher as the level of physical activity increased (low level 49.5 ± 14%, moderate level 55 ± 12%, intense level 69 ± 20%).
Do Chest Pain Characteristics in Patients with Acute Myocardial Infarction Differ between Those with and without Obstructive Coronary Artery Disease?
Authors: Pasupathy Sivabaskari , La Sarena , Tavella Rosanna , Zeitz Christopher , Worthley Matthew , Sinhal Ajay , Arstall Margaret , Beltrame John
Published: July 2023

The universal definition of acute myocardial infarction (MI) requires both evidence of myocardial injury and myocardial ischaemia. In MINOCA (MI with non-obstructive coronary arteries), patients must fulfil this MI criteria, but is their chest pain similar to those who have MI with obstructive CAD (MICAD)? This study compares prospectively collected chest pain features between patients with MINOCA and MICAD. Utilising the Coronary Angiogram Database of South Australia (CADOSA), consecutive MI patients were categorized as MINOCA or MICAD based on angiographic findings. Chest pain data were collected via direct patient interviews by trained staff members. Of 6811 consecutive patients fulfilling a clinical MI diagnosis, 411 (6.0%) were MINOCA, and 5948 MICAD. The MINOCA patients were younger, more often female and had less cardiovascular risk factors than those with MICAD. There were no significant differences in chest pain characteristics between the MINOCA and MICAD cohorts in relation to pain location, quality, associated symptoms, or duration. In conclusion, MINOCA patients have chest pain characteristics that are indistinguishable from MICAD patients, suggesting that their pain is ischaemic in nature. Thus, in the presence of positive myocardial injury markers, ischaemic chest pain fulfils the universal criteria for MI, despite the absence of obstructive coronary artery disease.
Relation between Maximum Oral Muscle Pressure and Dentoalveolar Characteristics in Patients with Cleft Lip and/or Palate: A Prospective Comparative Study
Authors: Van Geneugden Lisa , Verdonck Anna , Willems Guy , Hens Greet , Cadenas de Llano-Pérula Maria
Published: July 2023

Background: Perioral muscle function, which influences maxillofacial growth and tooth position, can be affected in patients with oral clefts due to their inherent anatomical characteristics and the multiple surgical corrections performed. This research aims to (1) compare the maximum oral muscle pressure of subjects with and without isolated cleft palate (CP) or unilateral cleft lip and palate (UCLP), (2) investigate its influence on their dentoalveolar characteristics, and (3) investigate the influence of functional habits on the maximum oral muscle pressure in patients with and without cleft. Material and methods: Subjects with and without CP and UCLP seeking treatment at the Department of Orthodontics of University Hospitals Leuven between January 2021 and August 2022 were invited to participate. The Iowa Oral Performance Instrument (IOPI) was used to measure their maximum tongue, lip, and cheek pressure. An imbalance score was calculated to express the relationship between tongue and lip pressure. Upper and lower intercanine (ICD) and intermolar distance (IMD) were measured on 3D digital dental casts, and the presence of functional habits was reported by the patients. The data were analyzed with multivariable linear models, correcting for age and gender. Results: 44 subjects with CP or UCLP (mean age: 12.00 years) and 104 non-affected patients (mean age: 11.13 years) were included. No significant differences in maximum oral muscle pressure or imbalance score were detected between controls and clefts or between cleft types. Significantly smaller upper ICDs and larger upper and lower IMDs were found in patients with clefts. A significant difference between controls and clefts was found in the relationship between oral muscle pressure and transversal jaw width. In cleft patients, the higher the maximum tongue pressure, the wider the upper and lower IMD, the higher the lip pressure, the smaller the upper and lower ICD and IMD, and the higher the imbalance score, the larger the upper and lower IMD and lower ICD. An imbalance favoring the tongue was found in cleft patients. The influence of functional habits on the maximum oral muscle pressure was not statistically different between clefts and controls. Conclusion: Patients with CP or UCLP did not present reduced maximum oral muscle pressure compared with patients without a cleft. In cleft patients, tongue pressure was consistently greater than lip pressure, and those who presented a larger maxillary width presented systematically higher imbalance scores (favoring the tongue) than those with narrow maxillae. Therefore, the influence of slow maxillary expansion on maximum oral muscle pressure in cleft patients should not be underestimated.