bluuebunny/crossref_metadata_2025 huggingface dataset:
INTACT vs. FANS for Cell-Type-Specific Nuclei Sorting: A Comprehensive Qualitative and Quantitative Comparison
Authors: Chongtham Monika Chanu , Butto Tamer , Mungikar Kanak , Gerber Susanne , Winter Jennifer
Published: May 2021

Increasing numbers of studies seek to characterize the different cellular sub-populations present in mammalian tissues. The techniques “Isolation of Nuclei Tagged in Specific Cell Types” (INTACT) or “Fluorescence-Activated Nuclei Sorting” (FANS) are frequently used for isolating nuclei of specific cellular subtypes. These nuclei are then used for molecular characterization of the cellular sub-populations. Despite the increasing popularity of both techniques, little is known about their isolation efficiency, advantages, and disadvantages or downstream molecular effects. In our study, we compared the physical and molecular attributes of sfGFP+ nuclei isolated by the two methods—INTACT and FANS—from the neocortices of Arc-CreERT2 × CAG-Sun1/sfGFP animals. We identified differences in efficiency of sfGFP+ nuclei isolation, nuclear size as well as transcriptional (RNA-seq) and chromatin accessibility (ATAC-seq) states. Therefore, our study presents a comprehensive comparison between the two widely used nuclei sorting techniques, identifying the advantages and disadvantages for both INTACT and FANS. Our conclusions are summarized in a table to guide researchers in selecting the most suitable methodology for their individual experimental design.
A New Mechanism in THRA Resistance: The First Disease-Associated Variant Leading to an Increased Inhibitory Function of THRA2
Authors: Paisdzior Sarah , Knierim Ellen , Kleinau Gunnar , Biebermann Heike , Krude Heiko , Straussberg Rachel , Schuelke Markus
Published: May 2021

The nuclear thyroid hormone receptors (THRs) are key mediators of thyroid hormone function on the cellular level via modulation of gene expression. Two different genes encode THRs (THRA and THRB), and are pleiotropically involved in development, metabolism, and growth. The THRA1 and THRA2 isoforms, which result from alternative splicing of THRA, differ in their C-terminal ligand-binding domain (LBD). Most published disease-associated THRA variants are located in the LBD of THRA1 and impede triiodothyronine (T3) binding. This keeps the nuclear receptor in an inactive state and inhibits target gene expression. Here, we investigated a new dominant THRA variant (chr17:g.38,241,010A > G, GRCh37.13 | c.518A > G, NM_199334 | p.(E173G), NP_955366), which is located between the DNA- and ligand-binding domains and affects both splicing isoforms. Patients presented partially with hypothyroid (intellectual disability, motor developmental delay, brain atrophy, and constipation) and partially with hyperthyroid symptoms (tachycardia and behavioral abnormalities) to varying degrees. Functional characterization of THRA1p.(E173G) by reporter gene assays revealed increased transcriptional activity in contrast to THRA1(WT), unexpectedly revealing the first gain-of-function mutation found in THRA1. The THRA2 isoform does not bind T3 and antagonizes THRA1 action. Introduction of p.(E173G) into THRA2 increased its inhibitory effect on THRA1, which helps to explain the hypothyroid symptoms seen in our patients. We used protein structure models to investigate possible underlying pathomechanisms of this variant with a gain-of-antagonistic function and suggest that the p.(E173G) variant may have an influence on the dimerization domain of the nuclear receptor.
Formulative Study and Intracellular Fate Evaluation of Ethosomes and Transethosomes for Vitamin D3 Delivery
Authors: Costanzo Manuela , Esposito Elisabetta , Sguizzato Maddalena , Lacavalla Maria Assunta , Drechsler Markus , Valacchi Giuseppe , Zancanaro Carlo , Malatesta Manuela
Published: May 2021

In this pilot study, ethosomes and transethosomes were investigated as potential delivery systems for cholecalciferol (vitamin D3), whose deficiency has been correlated to many disorders such as dermatological diseases, systemic infections, cancer and sarcopenia. A formulative study on the influence of pharmaceutically acceptable ionic and non-ionic surfactants allowed the preparation of different transethosomes. In vitro cytotoxicity was evaluated in different cell types representative of epithelial, connective and muscle tissue. Then, the selected nanocarriers were further investigated at light and transmission electron microscopy to evaluate their uptake and intracellular fate. Both ethosomes and transethosomes proven to have physicochemical properties optimal for transdermal penetration and efficient vitamin D3 loading; moreover, nanocarriers were easily internalized by all cell types, although they followed distinct intracellular fates: ethosomes persisted for long times inside the cytoplasm, without inducing subcellular alteration, while transethosomes underwent rapid degradation giving rise to an intracellular accumulation of lipids. These basic results provide a solid scientific background to in vivo investigations aimed at exploring the efficacy of vitamin D3 transdermal administration in different experimental and pathological conditions.
The Low-Molecular Weight Protein Arginine Phosphatase PtpB Affects Nuclease Production, Cell Wall Integrity, and Uptake Rates of Staphylococcus aureus by Polymorphonuclear Leukocytes
Authors: Elhawy Mohamed Ibrahem , Molle Virginie , Becker Sören L. , Bischoff Markus
Published: May 2021

The epidemiological success of Staphylococcus aureus as a versatile pathogen in mammals is largely attributed to its virulence factor repertoire and the sophisticated regulatory network controlling this virulon. Here we demonstrate that the low-molecular-weight protein arginine phosphatase PtpB contributes to this regulatory network by affecting the growth phase-dependent transcription of the virulence factor encoding genes/operons aur, nuc, and psmα, and that of the small regulatory RNA RNAIII. Inactivation of ptpB in S. aureus SA564 also significantly decreased the capacity of the mutant to degrade extracellular DNA, to hydrolyze proteins in the extracellular milieu, and to withstand Triton X-100 induced autolysis. SA564 ΔptpB mutant cells were additionally ingested faster by polymorphonuclear leukocytes in a whole blood phagocytosis assay, suggesting that PtpB contributes by several ways positively to the ability of S. aureus to evade host innate immunity.
Not Just a Bystander: The Emerging Role of Astrocytes and Research Tools in Studying Cognitive Dysfunctions in Schizophrenia
Authors: Chang Chia-Yuan , Luo Da-Zhong , Pei Ju-Chun , Kuo Ming-Che , Hsieh Yi-Chen , Lai Wen-Sung
Published: May 2021

Cognitive dysfunction is one of the core symptoms in schizophrenia, and it is predictive of functional outcomes and therefore useful for treatment targets. Rather than improving cognitive deficits, currently available antipsychotics mainly focus on positive symptoms, targeting dopaminergic/serotoninergic neurons and receptors in the brain. Apart from investigating the neural mechanisms underlying schizophrenia, emerging evidence indicates the importance of glial cells in brain structure development and their involvement in cognitive functions. Although the etiopathology of astrocytes in schizophrenia remains unclear, accumulated evidence reveals that alterations in gene expression and astrocyte products have been reported in schizophrenic patients. To further investigate the role of astrocytes in schizophrenia, we highlighted recent progress in the investigation of the effect of astrocytes on abnormalities in glutamate transmission and impairments in the blood–brain barrier. Recent advances in animal models and behavioral methods were introduced to examine schizophrenia-related cognitive deficits and negative symptoms. We also highlighted several experimental tools that further elucidate the role of astrocytes. Instead of focusing on schizophrenia as a neuron-specific disorder, an additional astrocytic perspective provides novel and promising insight into its causal mechanisms and treatment. The involvement of astrocytes in the pathogenesis of schizophrenia and other brain disorders is worth further investigation.
Glia and Orofacial Pain: Progress and Future Directions
Authors: Ye Yi , Salvo Elizabeth , Romero-Reyes Marcela , Akerman Simon , Shimizu Emi , Kobayashi Yoshifumi , Michot Benoit , Gibbs Jennifer
Published: May 2021

Orofacial pain is a universal predicament, afflicting millions of individuals worldwide. Research on the molecular mechanisms of orofacial pain has predominately focused on the role of neurons underlying nociception. However, aside from neural mechanisms, non-neuronal cells, such as Schwann cells and satellite ganglion cells in the peripheral nervous system, and microglia and astrocytes in the central nervous system, are important players in both peripheral and central processing of pain in the orofacial region. This review highlights recent molecular and cellular findings of the glia involvement and glia–neuron interactions in four common orofacial pain conditions such as headache, dental pulp injury, temporomandibular joint dysfunction/inflammation, and head and neck cancer. We will discuss the remaining questions and future directions on glial involvement in these four orofacial pain conditions.
Exosome Traceability and Cell Source Dependence on Composition and Cell-Cell Cross Talk
Authors: Hamzah Rabab N. , Alghazali Karrer M. , Biris Alexandru S. , Griffin Robert J.
Published: May 2021

Exosomes are small vesicles with an average diameter of 100 nm that are produced by many, if not all, cell types. Exosome cargo includes lipids, proteins, and nucleic acids arranged specifically in the endosomes of donor cells. Exosomes can transfer the donor cell components to target cells and can affect cell signaling, proliferation, and differentiation. Important new information about exosomes’ remote communication with other cells is rapidly being accumulated. Recent data indicates that the results of this communication depend on the donor cell type and the environment of the host cell. In the field of cancer research, major questions remain, such as whether tumor cell exosomes are equally taken up by cancer cells and normal cells and whether exosomes secreted by normal cells are specifically taken up by other normal cells or also tumor cells. Furthermore, we do not know how exosome uptake is made selective, how we can trace exosome uptake selectivity, or what the most appropriate methods are to study exosome uptake and selectivity. This review will explain the effect of exosome source and the impact of the donor cell growth environment on tumor and normal cell interaction and communication. The review will also summarize the methods that have been used to label and trace exosomes to date.
Bioassay Development for Bispecific Antibodies—Challenges and Opportunities
Authors: Register Ames C. , Tarighat Somayeh S. , Lee Ho Young
Published: May 2021

Antibody therapeutics are expanding with promising clinical outcomes, and diverse formats of antibodies are further developed and available for patients of the most challenging disease areas. Bispecific antibodies (BsAbs) have several significant advantages over monospecific antibodies by engaging two antigen targets. Due to the complicated mechanism of action, diverse structural variations, and dual-target binding, developing bioassays and other types of assays to characterize BsAbs is challenging. Developing bioassays for BsAbs requires a good understanding of the mechanism of action of the molecule, principles and applications of different bioanalytical methods, and phase-appropriate considerations per regulatory guidelines. Here, we review recent advances and case studies to provide strategies and insights for bioassay development for different types of bispecific molecules.
Ribosome Protection Proteins—“New” Players in the Global Arms Race with Antibiotic-Resistant Pathogens
Authors: Ero Rya , Yan Xin-Fu , Gao Yong-Gui
Published: May 2021

Bacteria have evolved an array of mechanisms enabling them to resist the inhibitory effect of antibiotics, a significant proportion of which target the ribosome. Indeed, resistance mechanisms have been identified for nearly every antibiotic that is currently used in clinical practice. With the ever-increasing list of multi-drug-resistant pathogens and very few novel antibiotics in the pharmaceutical pipeline, treatable infections are likely to become life-threatening once again. Most of the prevalent resistance mechanisms are well understood and their clinical significance is recognized. In contrast, ribosome protection protein-mediated resistance has flown under the radar for a long time and has been considered a minor factor in the clinical setting. Not until the recent discovery of the ATP-binding cassette family F protein-mediated resistance in an extensive list of human pathogens has the significance of ribosome protection proteins been truly appreciated. Understanding the underlying resistance mechanism has the potential to guide the development of novel therapeutic approaches to evade or overcome the resistance. In this review, we discuss the latest developments regarding ribosome protection proteins focusing on the current antimicrobial arsenal and pharmaceutical pipeline as well as potential implications for the future of fighting bacterial infections in the time of “superbugs.”
Edible Insects and Global Food Security
Authors: Verneau Fabio , Amato Mario , La Barbera Francesco La
Published: May 2021

Starting in 2008 and lasting up until 2011, the crisis in agricultural and, in particular, cereal prices triggered a period of riots that spread from the Mediterranean basin to the rest of the world, reaching from Asia to Central America and the African continent. [...]
Adult Feeding Preference and Fecundity in the Large Pine Weevil, Hylobius abietis (Coleoptera: Curculionidae)
Authors: Doležal Petr , Kleinová Lenka , Davídková Markéta
Published: May 2021

Adult feeding preferences of Hylobius abietis on Picea abies, Larix decidua, Pinus sylvestris, Pseudotsuga menziesii, Abies alba and Betula pendula were tested in outdoor conditions. The preferred food source was P. menziesii, and the mean bark area consumed per seedling was 440.8 ± 147.9 mm2. The second most preferred host was P. abies. The coniferous species that suffered the least damage was A. alba (76.8 ± 62.56 mm2 per seedling). B. pendula was the least preferred source of food, and it caused mortality of 60% of weevils that fed on it. Weevils exhibited large differences in fecundity when fed with different tree species in a laboratory experiment. The largest number of eggs was laid by females fed with P. abies. Mean egg numbers reached 26.4 ± 24.89 eggs per experiment for P. abies. Similar fecundity was observed in weevils fed with twigs of P. sylvestris. Oviposition was approximately six times lower in females fed with L. decidua and P. menziesii. The maximum number of eggs laid by a single female during a one-month experiment was 90. The results are discussed in relation to management of H. abietis.
Targeting the Complement Cascade in the Pathophysiology of COVID-19 Disease
Authors: Ng Nicole , Powell Charles A.
Published: May 2021

Severe coronavirus disease 2019 causes multi-organ dysfunction with significant morbidity and mortality. Mounting evidence implicates maladaptive over-activation of innate immune pathways such as the complement cascade as well as endothelial dysfunction as significant contributors to disease progression. We review the complement pathways, the effects of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on these pathways, and promising therapeutic targets in clinical trials.
Needling Interventions for Sciatica: Choosing Methods Based on Neuropathic Pain Mechanisms—A Scoping Review
Authors: Perreault Thomas , Fernández-de-las-Peñas César , Cummings Mike , Gendron Barry C.
Published: May 2021

Sciatica is a condition often accompanied by neuropathic pain (NP). Acupuncture and dry needling are common treatments for pain, and the current literature supports acupuncture as an effective treatment for sciatica. However, it is unknown if the mechanisms of NP are considered in the delivery of needling interventions for sciatica. Our objective was to assess the efficacy and the effectiveness of needling therapies, to identify common needling practices and to investigate if NP mechanisms are considered in the treatment of sciatica. A scoping review of the literature on needling interventions for sciatica and a review of the literature on mechanisms related to NP and needling interventions were performed. Electronic literature searches were conducted on PubMed, MEDLINE, CINAHL and Cochrane Database of Systematic Reviews from inception to August, 2020 to identify relevant papers. Reference lists of included papers were also manually screened and a related-articles search through PubMed was performed on all included articles. Mapping of the results included description of included studies, summary of results, and identification of gaps in the existing literature. Ten articles were included. All studies used acupuncture for the treatment of sciatica, no studies on dry needling were identified. Current evidence supports the efficacy and effectiveness of acupuncture for sciatica, however, no studies considered underlying NP mechanisms in the acupuncture approach for sciatica and the rationale for using acupuncture was inconsistent among trials. This review reveals that neuropathic pain mechanisms are not routinely considered in needling approaches for patients with sciatica. Studies showed acupuncture to be an effective treatment for sciatic pain, however, further research is warranted to explore if needling interventions for sciatica and NP would be more effective if NP mechanisms are considered.
Glaucoma Progression after Delivery in Patients with Open-Angle Glaucoma Who Discontinued Glaucoma Medication during Pregnancy
Authors: Seo Duri , Lee Taekjune , Kim Joo Yeon , Seong Gong Je , Choi Wungrak , Bae Hyong Won , Kim Chan Yun
Published: May 2021

In this retrospective study, clinical characteristics and glaucoma progression of open-angle glaucoma (OAG) patients who discontinued intraocular pressure (IOP)-lowering medication during pregnancy were investigated. Glaucoma progression was determined using either serial visual field tests or optic disc/retinal nerve fiber layer (RNFL) photographs. Age, number of previous pregnancies, diagnosis, average IOP, IOP fluctuation, visual field mean deviation, pattern standard deviation, and RNFL thickness were examined, and their association with glaucoma progression was determined using linear regression analysis. Among 67 eyes (37 patients), 19 eyes (28.4%) exhibited glaucoma progression 13.95 ± 2.42 months after delivery. The progression group showed significantly higher mean IOP than the nonprogression group in the first, second, and third trimesters (p = 0.02, 0.001, and 0.04, respectively). The average IOP in the second, and third trimesters and IOP fluctuation during the entire pregnancy were significantly associated with glaucoma progression according to a univariate analysis (p = 0.04, 0.031, and 0.026, respectively). In conclusion, IOP elevation during pregnancy is associated with glaucoma progression after delivery in patients who had discontinued medication during pregnancy. Therefore, close monitoring of glaucoma is necessary, particularly if patients discontinue medication during pregnancy, and appropriate intervention should be considered in case of increased IOP.
Prognostic Role of Immune Checkpoint Regulators in Cholangiocarcinoma: A Pilot Study
Authors: Cao Lu , Prithviraj Prashanth , Shrestha Ritu , Sharma Revati , Anaka Matthew , Bridle Kim R. , Kannourakis George , Crawford Darrell H.G. , Jayachandran Aparna
Published: May 2021

Cholangiocarcinoma (CCA) is a hepatobiliary malignancy associated with steadily increasing incidence and poor prognosis. Ongoing clinical trials are assessing the effectiveness and safety of a few immune checkpoint inhibitors (ICIs) in CCA patients. However, these ICI treatments as monotherapies may be effective for a proportion of patients with CCA. The prevalence and distribution of other immune checkpoints (ICs) in CCA remain unclear. In this pilot study, we screened databases of CCA patients for the expression of 19 ICs and assessed the prognostic significance of these ICs in CCA patients. Notably, expression of immune modulator IDO1 and PD-L1 were linked with poor overall survival, while FASLG and NT5E were related to both worse overall survival and progression-free survival. We also identified immune modulators IDO1, FASLG, CD80, HAVCR2, NT5E, CTLA-4, LGALS9, VTCN1 and TNFRSF14 that synergized with PD-L1 and correlated with worse patient outcomes. In vitro studies revealed that the expression of ICs was closely linked with aggressive CCA subpopulations, such as cancer stem cells and cells undergoing TGF-β and TNF-α-mediated epithelial-to-mesenchymal transition. These findings suggest that the aforementioned IC molecules may serve as potential prognostic biomarkers and drug targets in CCA patients, leading to lasting and durable treatment outcomes.
Supervised Resistance Training on Functional Capacity, Muscle Strength and Vascular Function in Peripheral Artery Disease: An Updated Systematic Review and Meta-Analysis
Authors: Blears Elizabeth E. , Elias Jessica K. , Tapking Christian , Porter Craig , Rontoyanni Victoria G.
Published: May 2021

Supervised resistance training appears to be a promising alternative exercise modality to supervised walking in patients with peripheral artery disease (PAD). This meta-analysis examined the efficacy of supervised RT for improving walking capacity, and whether adaptations occur at the vascular and/or skeletal muscle level in PAD patients. We searched Medline, CINAHL, Scopus, and Cochrane Central Register of Controlled Trials databases for randomized controlled trials (RCTs) in PAD patients testing the effects of supervised RT for ≥4 wk. on walking capacity, vascular function, and muscle strength. Pooled effect estimates were calculated and evaluated using conventional meta-analytic procedures. Six RCTs compared supervised RT to standard care. Overall, supervised RT prolonged claudication onset distance during a 6-min walk test (6-MWT) (101.7 m (59.6, 143.8), p < 0.001) and improved total walking distance during graded treadmill walking (SMD: 0.67 (0.33, 1.01), p < 0.001) and the 6-MWT (49.4 m (3.1, 95.6), p = 0.04). Five RCTS compared supervised RT and supervised intermittent walking, where the differences in functional capacity between the two exercise modalities appear to depend on the intensity of the exercise program. The insufficient evidence on the effects of RT on vascular function and muscle strength permitted only limited exploration. We conclude that RT is effective in prolonging walking performance in PAD patients. Whether RT exerts its influence on functional capacity by promoting blood flow and/or enhancing skeletal muscle strength remains unclear.
Association of Perioperative Regional Analgesia with Postoperative Patient-Reported Pain Outcomes and Opioid Requirements: Comparing 22 Different Surgical Groups in 23,911 Patients from the QUIPS Registry
Authors: Komann Marcus , Avian Alexander , Dreiling Johannes , Gerbershagen Hans , Volk Thomas , Weinmann Claudia , Meißner Winfried
Published: May 2021

(1) Background: In many surgical procedures, regional analgesia (RA) techniques are associated with improved postoperative analgesia compared to systemic pain treatment. As continuous RA requires time and experienced staff, it would be helpful to identify settings in which continuous RA has the largest benefit. (2) Methods: On the basis of 23,911 data sets from 179 German and Austrian hospitals, we analyzed the association of perioperative RA with patient-reported pain intensity, functional impairment of movement, nausea and opioid use for different surgeries. Regression analyses adjusted for age, sex and preoperative pain were performed for each surgery and the following groups: patients receiving continuous RA (surgery and ward; RA++), RA for surgery only (RA+−) and patients receiving no RA (RA−−). (3) Results: Lower pain scores in the RA++ compared to the RA−− group were observed in 13 out of 22 surgeries. There was no surgery where pain scores for RA++ were higher than for RA−−. If maximal pain, function and side effects were combined, the largest benefit of continuous RA (RA++) was observed in laparoscopic colon and sigmoid surgery, ankle joint arthrodesis, revision (but not primary) surgery of hip replacement, open nephrectomy and shoulder surgery. The benefit of RA+− was lower than that of RA++. (4) Discussion: The additional benefit of RA for the mentioned surgeries is larger than in many other surgeries in clinical routine. The decision to use RA in a given surgery should be based on the expected pain intensity without RA and its additional benefits.
Characteristics and Risk Factors for Intensive Care Unit Cardiac Arrest in Critically Ill Patients with COVID-19—A Retrospective Study
Authors: Roedl Kevin , Söffker Gerold , Wichmann Dominic , Boenisch Olaf , de Heer Geraldine , Burdelski Christoph , Frings Daniel , Sensen Barbara , Nierhaus Axel , Westermann Dirk , Kluge Stefan , Jarczak Dominik
Published: May 2021

The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) causing the coronavirus disease 2019 (COVID-19) led to an ongoing pandemic with a surge of critically ill patients. Very little is known about the occurrence and characteristic of cardiac arrest in critically ill patients with COVID-19 treated at the intensive care unit (ICU). The aim was to investigate the incidence and outcome of intensive care unit cardiac arrest (ICU-CA) in critically ill patients with COVID-19. This was a retrospective analysis of prospectively recorded data of all consecutive adult patients with COVID-19 admitted (27 February 2020–14 January 2021) at the University Medical Centre Hamburg-Eppendorf (Germany). Of 183 critically ill patients with COVID-19, 18% (n = 33) had ICU-CA. The median age of the study population was 63 (55–73) years and 66% (n = 120) were male. Demographic characteristics and comorbidities did not differ significantly between patients with and without ICU-CA. Simplified Acute Physiological Score II (SAPS II) (ICU-CA: median 44 points vs. no ICU-CA: 39 points) and Sequential Organ Failure Assessment (SOFA) score (median 12 points vs. 7 points) on admission were significantly higher in patients with ICU-CA. Acute respiratory distress syndrome (ARDS) was present in 91% (n = 30) with and in 63% (n = 94) without ICU-CA (p = 0.002). Mechanical ventilation was more common in patients with ICU-CA (97% vs. 67%). The median stay in ICU before CA was 6 (1–17) days. A total of 33% (n = 11) of ICU-CAs occurred during the first 24 h of ICU stay. The initial rhythm was non-shockable (pulseless electrical activity (PEA)/asystole) in 91% (n = 30); 94% (n = 31) had sustained return of spontaneous circulation (ROSC). The median time to ROSC was 3 (1–5) minutes. Patients with ICU-CA had significantly higher ICU mortality (61% vs. 37%). Multivariable logistic regression showed that the presence of ARDS (odds ratio (OR) 4.268, 95% confidence interval (CI) 1.211–15.036; p = 0.024) and high SAPS II (OR 1.031, 95% CI 0.997–1.065; p = 0.077) were independently associated with the occurrence of ICU-CA. A total of 18% of critically ill patients with COVID-19 suffered from a cardiac arrest within the intensive care unit. The occurrence of ICU-CA was associated with presence of ARDS and severity of illness.
Quality of Life of Patients after Kinesio Tape Applications Following Impacted Mandibular Third Molar Surgeries
Authors: Jaroń Aleksandra , Preuss Olga , Konkol Berenika , Trybek Grzegorz
Published: May 2021

Today, extraction of the impacted third molar is the most common procedure performed in oral surgery departments. One of the methods currently investigated—in terms reducing the severity of non-infectious complications and decreasing Quality of Life following third molar surgeries—is Kinesio Taping (KT). The aim of the study was to evaluate the impact of Kinesio Tape application on Quality of Life. A total of 100 asymptomatic patients with impacted third lower molar were included. The study participants were randomly divided into two groups: a study group with the application of KT (n = 50) and a control group (without KT) (n = 50). Removal of the impacted third lower molar was performed in each patient in a standardized fashion. For assessment of Quality of Life, the modified University of Washington Quality of Life Questionnaire (UW-QoL v4) was used. Patients with Kinesio Tape application scored higher in all domains. Statistically significant differences between the two groups were found in the following domains: “Activity”, “Mood”, “Health-related QoL during the past 7 days” and “Overall QoL during the past 7 days”. There were no significant differences in significant problems and important issues between groups. Kinesio Taping has a significant impact on Quality of Life after impacted third molar removal. It should be considered as one of the noninvasive methods to reduce postoperative non-infectious complications.
End-to-End Deep One-Class Learning for Anomaly Detection in UAV Video Stream
Authors: Hamdi Slim , Bouindour Samir , Snoussi Hichem , Wang Tian , Abid Mohamed
Published: May 2021

In recent years, the use of drones for surveillance tasks has been on the rise worldwide. However, in the context of anomaly detection, only normal events are available for the learning process. Therefore, the implementation of a generative learning method in an unsupervised mode to solve this problem becomes fundamental. In this context, we propose a new end-to-end architecture capable of generating optical flow images from original UAV images and extracting compact spatio-temporal characteristics for anomaly detection purposes. It is designed with a custom loss function as a sum of three terms, the reconstruction loss (Rl), the generation loss (Gl) and the compactness loss (Cl) to ensure an efficient classification of the “deep-one” class. In addition, we propose to minimize the effect of UAV motion in video processing by applying background subtraction on optical flow images. We tested our method on very complex datasets called the mini-drone video dataset, and obtained results surpassing existing techniques’ performances with an AUC of 85.3.
Effect of Modelling Inhomogeneous Wave Conditions on Structural Responses of a Very Long Floating Bridge
Authors: Dai Jian , Stefanakos Christos , Leira Bernt J. , Alsos Hagbart Skage
Published: May 2021

Floating bridges are suitable for connecting land parcels separated by wide and deep waterbodies. However, when the span of the crossing becomes very long, the water environment exhibits inhomogeneities which introduce difficulties to the modelling, analysis and design of the bridge structure. The wave inhomogeneity may be described by means of field measurement and/or numerical simulations. Both approaches face complications when the resolution is much refined. It is thus important to examine the effect of the resolution related to the modelling of inhomogeneous waves on the global structural responses. In this study, a hypothetical crossing at the Sulafjord is chosen, and the wave environment in the year 2015 at 10 positions along the crossing is numerically computed. Next, different inhomogeneous wave conditions are established based on the wave data at 3, 5, and 10 positions, respectively. Time-domain simulations are conducted to examine the effect of different modelling approaches of the inhomogeneous wave condition on the global responses of a long, straight and side-anchored floating bridge.
Dynamics and Distribution of Marine Synechococcus Abundance and Genotypes during Seasonal Hypoxia in a Coastal Marine Ranch
Authors: Li Guihao , Song Qinqin , Zheng Pengfei , Zhang Xiaoli , Zou Songbao , Li Yanfang , Gao Xuelu , Zhao Zhao , Gong Jun
Published: May 2021

Marine Synechococcus are an ecologically important picocyanobacterial group widely distributed in various oceanic environments. Little is known about the dynamics and distribution of Synechococcus abundance and genotypes during seasonal hypoxia in coastal zones. In this study, an investigation was conducted in a coastal marine ranch along two transects in Muping, Yantai, where hypoxic events (defined here as the dissolved oxygen concentration <3 mg L−1) occurred in the summer of 2015. The hypoxia occurred in the bottom waters from late July and persisted until late August. It was confined at nearshore stations of the two transects, one running across a coastal ranch and the other one outside. During this survey, cell abundance of Synechococcus was determined with flow cytometry, showing great variations ranging from 1 × 104 to 3.0 × 105 cells mL−1, and a bloom of Synechococcus occurred when stratification disappeared and hypoxia faded out outside the ranch. Regression analysis indicated that dissolved oxygen, pH, and inorganic nutrients were the most important abiotic factors in explaining the variation in Synechococcus cell abundance. Diverse genotypes (mostly belonged to the sub-clusters 5.1 and 5.2) were detected using clone library sequencing and terminal restriction fragment length polymorphism analysis of the 16S–23S rRNA internal transcribed spacer region. The richness of genotypes was significantly related to salinity, temperature, silicate, and pH, but not dissolved oxygen. Two environmental factors, temperature and salinity, collectively explained 17% of the variation in Synechococcus genotype assemblage. With the changes in population composition in diverse genotypes, the Synechococcus assemblages survived in the coastal hypoxia event and thrived when hypoxia faded out.
Occurrence and Anastomosis Grouping of Rhizoctonia spp. Inducing Black Scurf and Greyish-White Felt-Like Mycelium on Carrot in Sweden
Authors: Marcou Shirley , Wikström Mariann , Ragnarsson Sara , Persson Lars , Höfte Monica
Published: May 2021

Carrots with different Rhizoctonia-like symptoms were found in the main Swedish carrot production areas from 2001–2020. The most commonly observed symptoms were a greyish-white felt-like mycelium and black scurf, the latter often associated with Rhizoctonia solani anastomosis group (AG) 3-PT on potato. An overall increase in disease incidence in all studied fields over time was observed for both symptoms. The majority of Rhizoctonia isolates sampled from carrot in the period 2015–2020 were identified as AG 3 (45%) and AG 5 (24%), followed by AG 1-IB (13%), AG 11 (5%), AG-E (5%), AG BI (3%), AG-K (3%) and AG 4-HGII (2%). To our knowledge, this is the first report describing AG 5 in Sweden as well as AG 3, AG 11 and AG-E inducing Rhizoctonia-like symptoms on carrot. Secondly, we report for the first time that R. solani AG 3, and the less observed AGs: AG 1-IB and AG 5 can induce black scurf symptoms on the taproot of carrots. Due to a widely used carrot-potato crop rotation in Swedish areas, a possible cross-over from potato to carrot is suggested. This information is of high importance to reduce Rhizoctonia inoculum in soils, since avoiding carrot-potato crop rotations needs to be considered.
Closed Churches during the Pandemic: Liberal versus Conservative and Christian versus Atheist Argumentation in Media
Authors: Rončáková Terézia
Published: May 2021

The current COVID-19 pandemic has led to the introduction of various epidemiological measures, including the ban on public worship. The problem of closed churches has become an intensely debated subject across several countries and a hotly debated question in recent media discourse. This paper provides an analysis of the arguments presented on the subject of closed churches by the media in Slovakia and the Czech Republic. In addition to the detailed analysis of the argumentation used, it also presents a twofold comparison: arguments presented in liberal versus conservative media, and arguments presented in the Slovak media versus Czech media. Twenty-eight years ago, these two countries were part of one state and after the split, the countries became a model of a peaceful dissolution (the so-called ‘velvet divorce’). However, from a religious perspective, they are quite different: whereas Slovakia is one of the most Christian (Catholic) countries, the Czech Republic is one of the most atheist countries in Europe. Three research dimensions are presented as part of this study: (1) media argumentation on the problem of closed churches; (2) comparison of liberal versus conservative arguments; (3) comparison of the media coverage in a strongly Christian country versus a strongly atheist country.
Polygenic Panels Predicting the Susceptibility of Multiple Upper Aerodigestive Tract Cancer in Oral Cancer Patients
Authors: Chien Huei-Tzu , Yeh Chi-Chin , Young Chi-Kuang , Chen Tzu-Ping , Liao Chun-Ta , Wang Hung-Ming , Cho Kai-Lun , Huang Shiang-Fu
Published: May 2021

Head and neck cancer was closely related with habitual use of cigarette and alcohol. Those cancer patients are susceptible to develop multiple primary tumors (MPTs). In this study, we utilized the single nucleotide polymorphisms (SNPs) array (Affymetrix Axion Genome-Wide TWB 2.0 Array Plate) to investigate patients’ risks of developing multiple primary cancers. We recruited 712 male head and neck cancer patients between Mar 1996 and Feb 2017. Two hundred and eighty-six patients (40.2%) had MPTs and 426 (59.8%) had single cancer. Four hundred and twelve normal controls were also recruited. A list of seventeen factors was extracted and ten factors were demonstrated to increase the risks of multiple primary cancers (alcohol drinking, rs118169127, rs149089400, rs76367287, rs61401220, rs141057871, rs7129229, older age, rs3760265, rs9554264; all were p value < 0.05). Polygenic scoring model was built and the area under curve to predict the risk developing MPTs is 0.906. Alcohol drinking, among the seventeen factors, was the most important risk factor to develop MPT in upper aerodigestive tract (OR: 7.071, 95% C.I.: 2.134–23.434). For those with high score in polygenic model, routine screening of upper digestive tract including laryngoscope and esophagoscope is suggested to detect new primaries early.
SARS-CoV-2-Laden Respiratory Aerosol Deposition in the Lung Alveolar-Interstitial Region Is a Potential Risk Factor for Severe Disease: A Modeling Study
Authors: Hofer Sabine , Hofstätter Norbert , Duschl Albert , Himly Martin
Published: May 2021

COVID-19, predominantly a mild disease, is associated with more severe clinical manifestation upon pulmonary involvement. Virion-laden aerosols and droplets target different anatomical sites for deposition. Compared to droplets, aerosols more readily advance into the peripheral lung. We performed in silico modeling to confirm the secondary pulmonary lobules as the primary site of disease initiation. By taking different anatomical aerosol origins into consideration and reflecting aerosols from exhalation maneuvers breathing and vocalization, the physicochemical properties of generated respiratory aerosol particles were defined upon conversion to droplet nuclei by evaporation at ambient air. To provide detailed, spatially-resolved information on particle deposition in the thoracic region of the lung, a top-down refinement approach was employed. Our study presents evidence for hot spots of aerosol deposition in lung generations beyond the terminal bronchiole, with a maximum in the secondary pulmonary lobules and a high preference to the lower lobes of both lungs. In vivo, initial chest CT anomalies, the ground glass opacities, resulting from partial alveolar filling and interstitial thickening in the secondary pulmonary lobules, are likewise localized in these lung generations, with the highest frequency in both lower lobes and in the early stage of disease. Hence, our results suggest a disease initiation right there upon inhalation of virion-laden respiratory aerosols, linking the aerosol transmission route to pathogenesis associated with higher disease burden and identifying aerosol transmission as a new independent risk factor for developing a pulmonary phase with a severe outcome.
Rethinking a Designers’ Rule of Thumb: Influence of Information Seeking and Consumption Goals on Mobile Commerce Interface Design
Authors: Hwang Sooa , Park Hyunah , Oh Kyunghui , Hwang Sangwoong , Joo Jaewoo
Published: May 2021

We investigated whether adding product information in mobile commerce improved consumers’ attitudes toward a product and whether this relationship was moderated by consumption goals. We conducted two field experiments in which we recruited parents in Korea and the USA and asked them how they evaluated two childcare hybrid products (HPs) newly developed by Samsung Electronics designers. The results revealed that participants exposed to additional information about the HPs evaluated them more favorably than those who were not exposed. However, this relationship disappeared when a consumption goal was activated. Our findings establish a dynamic relationship between information seeking and consumption goals, asking designers to rethink their rule of thumb in the mobile commerce context.
Attribution Analysis of Seasonal Runoff in the Source Region of the Yellow River Using Seasonal Budyko Hypothesis
Authors: Ji Guangxing , Wu Leying , Wang Liangdong , Yan Dan , Lai Zhizhu
Published: May 2021

Previous studies mainly focused on quantifying the contribution rate of different factors on annual runoff variation in the source region of the Yellow River (SRYR), while there are few studies on the seasonal runoff variation. In this study, the monthly water storage and monthly actual evaporation of SRYR were calculated by the monthly ABCD model, and then a seasonal Budyko frame was constructed. Finally, the contribution rate of climatic and anthropic factors on the seasonal runoff variation in Tangnaihai hydrological station were quantitatively calculated. It turned out that: (1) The changing point of runoff data at Tangnaihai hydrological station is 1989. (2) The ABCD monthly hydrological model could well simulate the monthly runoff variation of Tangnaihai hydrological station. (3) Anthropic factors play a major role in runoff change in spring, summer, and winter, while climatic factors play a major role in runoff change in autumn.
Multilingual Behaviour within the Portuguese and Italian Communities in Montreal: A Quest of Purism
Authors: Scetti Fabio
Published: May 2021

Many heritage speakers, starting particularly from the second generation, return to the practice of their heritage languages so as to build or rebuild their diasporic and heritage identities. Within an urban context such as in Montreal (Quebec), multilingual behaviour exists. This is due to the presence of multiple languages and dialects, as well as the bilingual reality of this city, where both French and English are dominant. Such conditions provide evidence of how determinant in-group ideologies and stereotypical attitudes are concerned with communities and languages (standard and vernacular) and how they function in the process of linguistic integration within the group and the Canadian city. Focusing on recent research that compares heritage speakers of Portuguese and Italian origin in Montreal, this contribution addresses whether identifying places have an important role in the process of integration within the group, in shared spaces of language or dialect practice, both private and public. Moreover, questions arise as to how standard languages are valued within both communities (mainly in schools) and how competency and legitimacy have been evaluated and by whom, in this process of integration. The two communities observed are very different, given the practice and behaviour as well as in-group ideologies of inclusion. This contribution argues that, as a consequence of our ‘global’ societies, there is an extension of new identities during the process of development where multilingual behaviour is reviewed and analysed for the dynamicity in the repertories of new generation speakers. Our comparison brings to light a central ideology of language purism, and the ways in which it is institutionalized and/or contested across the two groups.
Zero-Valent Iron Nanoparticles Remediate Nickel-Contaminated Aqueous Solutions and Biosolids-Amended Agricultural Soil
Authors: Mahdy Ahmed M. , Zhang Tiequan , Lin Zhi-Qing , Fathi Nieven O. , Badr Eldin Rasha M.
Published: May 2021

Nickel (Ni+2) accumulation in wastewater treatment sludge poses a potential environmental risk with biosolids-land application. An incubation experiment was conducted to evaluate the effect of nanoparticles of zero-valent iron (nZVI) on Ni+2 sorption in biosolids-treated agricultural soils. Two application rates of biosolids (0, 5%, w/w) and four treatment levels (0, 1, 5, and 10 g/kg) of nZVI were examined, either separately or interactively. The results of this study showed significant differences in Ni+2 sorption capacity between different nZVI treatments. The initial Ni+2 concentration in biosolids-amended soil significantly affected Ni sorption in the soil treated with nZVI. The “H-shape” of sorption isotherm in nZVI-treated soil reflects strong interaction between the Ni concentration and the nZVI treatment, while the C-shape of sorption isotherm in biosolids-amended soil without the nZVI treatment indicates intermediate affinity for Ni+2 sorption. Nickel retention in soil was increased with the increase of nZVI levels. The removal efficiency of Ni+2 by nZVI from solution was increased with the increase of pH from 5 to 11 and reached a maximum of 99.56% at pH 11 and nZVI treatment of 10 g/kg. The Ni+2 desorption rate decreased from 92 to 7, 4, and 1% with increasing nZVI treatment levels from 0 to 1, 5, and 10 g/kg, respectively, with a soil Ni+2 concentration of 50 mg/L. The maximum adsorption capacity (?max) of 10 g/kg nZVI-treated soil was 333.3 mg/g, which was much higher than those from the other treatments of 0 (5 mg/g), 1 (25 mg/g), and 5 g/kg (125 mg/g). The underlying mechanism for Ni+2 immobilization using nZVI in an aquatic environment is controlled by a sorption process, reduction of metal ion to zero-valent metal, as well as (co)precipitation. Moreover, increasing the nZVI treatment level in biosolids-amended soil significantly decreased bioavailable Ni+2 concentrations in the soil.
New Spin-Crossover Compounds Containing the [Ni(mnt)] Anion (mnt = Maleonitriledithiolate)
Authors: Turner Scott S. , Daniell Joanna , Akutsu Hiroki , Horton Peter N. , Coles Simon J. , Schünemann Volker
Published: May 2021

Two novel salts containing the anion [Ni(mnt)2]− (mnt = maleonitriledithiolate) have been synthesized. The counter-ions, [Fe(II)(L1 or L2)2], are cationic complexes where L1 and L2 are methylated derivatives of 2,6-bis(pyazolyl)pyridine or pyrazine, which are similar to ligands found in a series of spin-crossover (SCO) complexes. Both salts are characterized by variable temperature single crystal X-ray diffraction and bulk magnetization measurements. Compound 1, [Fe(II)(L1)2][Ni(mnt)2]2 displays an incomplete and gradual SCO up to 300 K, followed by a more rapid increase in the high-spin fraction between 300 and 350 K. Compound 2, [Fe(II)(L2)2][Ni(mnt)2]2.MeNO2, shows a gradual, but more complete SCO response centered at 250 K. For compound 2, the SCO is confirmed by variable temperature Mössbauer spectroscopy. In both cases, the anionic moieties are isolated from each other and so no electrical conductivity is observed.