bluuebunny/crossref_metadata_2025 huggingface dataset:
EPITOPES IDENTIFICATION OF BROADLY NEUTRALIZING MONOCLONAL ANTIBODIES AGAINST Corynebacterium diphtheriae EXOTOXIN
Authors:   Taras Shevchenko National University of Kyiv, Ukraine, Kulyk Ya.S. ,   Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, Kyiv
Published: November 2022

Background. Better and high-potency vaccines against diphtheria are urgently needed to provide broader protection against diverse strains and subtypes. Identification of novel broadly neutralizing epitopes targeted by protective antibodies could aid in such efforts. Aim. In this study we focused on the search of binding sites identification of anti diphtheria toxin monoclonal antibodies and their neutralizing activity to block binding of recombinant exotoxin derivates with host receptors. Methods. Vero cells were cultured in the complete RPMI-1640 medium under standard conditions and used for flow cytometry assay. Recombinant antigens and products of tryptic hydrolysis of CRM197 and SbB were characterized by Ni2+-NTA affinity chromatography and SDS-PAGE under reducing conditions with following ECL Western-Blot using several hybridomas clones of anti-diphtheria toxin monoclonal antibodies. Results. ECL western blot film results for clone 9.1-E11 showed the specific binding both to whole CRM197 molecule, and to almost all fragments of CRM197 formed as a result of limited proteolysis. In particular, a band corresponding to SbB in molecular weight can be identified. Thus, epitope region of the CRM197 molecule specific to 9.1-E1 mAbs is located within the structure of SbB. At the same time 16.4-E9 clone antibodies had high specificity to R-domain of SbB. In addition, both hybridoma clones antibodies have neutralizing activity against the DT binding subunit, which is a key factor in blocking between cell receptor and it ligand, C.diphtheriae exotoxin. Conclusions. The results obtained indicate that obtained antibodies are prospective for improving new diagnostic tools and therapeutic agents, which are used for treatment and understanding of the molecular mechanisms of diphtheria pathogenesis.
EFFECTS OF B. Subtilis IMV В-7724 LECTIN ON MALIGNANT AND NORMAL CELLS in vitro
Authors:   Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of the National Academy of Sciences of Ukraine, Kyiv, Symchych T.V.
Published: November 2022

Cancer cells upregulate surface expression of N-glycolyl-neuraminic acid (Neu5Gc). At Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology (IEPOR) of the National Academy of Science of Ukraine, B. subtilis IMV В-7724 lectin specific for Neu5Gc was obtained. Aim. The scope of the research was to study in vitro B. subtilis IMV В-7724 lectin activity towards malignant and normal cells. Materials and methods. Cytotoxic and mitogenic activities was studied by, respectively, MTT-assay and in vitro lymphocytes proliferation assay. Results. The lectin possesses cytotoxic activity towards human (А549, HL60) and murine (Ehrlich carcinoma, L1210) cancer cell lines. The most sensitive were L1210 and Ehrlich carcinoma cell lines. IC50 was 0.16 mg/ml in both cases. The lectin was less cytotoxic to murine peritoneal macrophages, lymphocytes and thymocytes: IC50 was 0.47, 2.02 and 3.49 mg/ml respectively. In a dose of 25 µg/ml the lectin induced lymphocytes proliferation. Conclusion. Depending on the target cells type and applied dose, B. subtilis IMV B-7724 lectin shows cytotoxic or mitogenic activities. Both of lectin’s activities can be applied in cancer treatment and thus deserve further investigation
Interstitial lung disease and neurodegeneration in FINCA syndrome: new knowledge changes old judgments
Authors:   I. Horbachevsky Ternopil National Medical University, Ukraine, Boyarchuk O.R.
Published: November 2022

FINCA (Fibrosis, Neurodegeneration, Cerebral Angiomatosis) syndrome is a new genetic multiorgan disease caused by a mutation in NHLRC2 (NHL repeat-containing protein 2). Disease manifests at an early age by interstitial lung disease, neurodegenerative disorders, hemolytic anemia, gastrointestinal disorders, liver dysfunction and other multiorgan changes. The syndrome was first described in 2018 in three children who died before the age of two. Studies in recent years have significantly expanded the range of clinical symptoms of FINCA syndrome, and also showed great variability in the severity of the symptomes, especially respiratory lesions. Taking into account the neurological problems in all described patients, NHLRC2 should be included in the sequencing panels of neurological diseases (neurodegenerative, neurodevelopmental disorders, epilepsy). Recurrent respiratory infections and episodic diarrhea, as well as hypogammaglobulinemia in most patients require study of the role of NHLRC2 protein in the functioning of the immune system. No conflict of interests was declared by the authors.
Toxoplasmic chorioretinitis in the adolescent: description of the own clinical case
Authors:   Donetsk National Medical University, Lуman, Ukraine, Khapchenkova D.S. , Husiev V.M. ,   Donetsk National Medical University, Lуman, Ukraine, Dubyna S.О. ,   Donetsk National Medical University, Lуman, Ukraine
Published: November 2022

Toxoplasmosis is a zoonotic protozoal disease caused by the intracellular parasite Toxoplasma gondii and is characterized by a polymorphism of clinical symptoms with predominant involvement of the nervous, lymphatic systems, eyes, skeletal muscles and myocardium. Despite the variety of therapeutic agents, the treatment of toxoplasmosis remains problematic and requires an interdisciplinary approach. Complete the release of toxoplasmas from the body is difficult, although theoretically possible. This process depends on the combination of individual characteristics of local and general immunity of the macroorganism, the amount of the pathogen and its virulence. Purpose - to present a clinical case of the patient with chorioretinitis as an example of a clinical manifestation of toxoplasmosis infection. Clinical case. The girl turned to an ophthalmologist with complaints of worsening vision and red eyes. After a doctor’s examination, an ophthalmoscopy was performed, the presence of two chorioretinal foci in both eyes was confirmed. Examination for TORCH-infection is recommended. An elevated level of IgM and IgG to Toxoplasma gondii was detected. The patient received a course of antibacterial and hormonal therapy with a positive effect. After 8 months, the girl’s complaints resumed, signs of synovitis of the knee joints appeared, and anemic syndrome joined. During repeated ophthalmoscopy, three chorioretinal foci were found. The girl was hospitalized in the ophthalmology department for a more detailed examination and to decide on further observation tactics. Conclusions. A case of chorioretinitis as a clinical manifestation of toxoplasmosis infection in adolescence is described. Timely diagnosis and started therapy of the above-described pathology are the key to a satisfactory prognosis for recovery, prevention of the development of negative consequences of this disease. The research was carried out in accordance with the principles of the Helsinki Declaration. The informed consent of the patient was obtained for conducting the studies. No conflict of interest was declared by the authors.
Juvenile systemic sclerosis in children (clinical case)
Authors:   Shupyk National Healthcare University of Ukraine, Kyiv, Taranenko Т.V. , Kozachuk V.G. ,   Shupyk National Healthcare University of Ukraine, Kyiv, Onufreiv O.E. ,   Shupyk National Healthcare University of Ukraine, Kyiv
Published: November 2022

The up-to-date data on origin, diagnostic approach and treatment of juvenile systemic scleroderma in the child with the disease onset at early age are presented in the article. The diagnostic value of anamnesis and clinical data, laboratory and instrumental methods of investigation, in particular, identification of juvenile systemic scleroderma immunological markers, ultrasound Doppler scan of vessels as well as capillaroscopy of the nail bed are covered. The main data of instrumental examinations for the diagnosis were pulmonary fibrosis, decreased cardiac ejection fraction of left ventricles, large-focal cardiosclerosis, thickening of the intima-media complex, signs of peripheral vascular Raynaud's phenomenon. The diagnostic laboratory markers are increased titers of antibodies to native and denatured DNA, antibodies to nucleosomes, antibodies to Scl-70, circulating immune complexes and CRP. The treatment regimen should be developed individually and be aimed at deceleration of the progression of the autoimmune process. The research was carried out in accordance with the principles of the Helsinki Declaration. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.
Determination of reducing carbohydrates in natural honey samples by optical micrometry method
Authors: Shchemelev Ivan S. , Khasanov Danil S. , Smirnova Maria A. , Ivanov Alexander V. , Ferapontov Nikolay B. , Tokmachev Mikhail G.
Published: November 2022

The present article deals with the determination of the total amount of glucose and fructose in natural honey samples by the optical micrometry (OM) method. (Poly)vinyl alcohol spherical granules impregnated with a 0.05 mol/l borax solution were chosen as a sensitive element. It was shown that the formation of chelate esters of boron with polymer and carbohydrates is a pH-dependent process, and that the pH range 8.5–10.0 is the most appropriate for quantifying the total amount of reducing carbohydrates because glucose and fructose are undiscriminated. The impregnated polymer is not sensitive to the sucrose presence in the solution due to the absence of cis-diol fragments in it. Subsequently, the OM method was tested in the analysis of natural honey samples. The relative standart deviation in the case of OM method is less than 6%, and the results are similar to those obtained by the iodometric titration method. This makes the OM method suitable for laboratory-scale applications.
Uma breve trajetória dos elementos constitutivos do Advocacy Coalition Framework (ACF)
Authors: Oliveira Henrique Campos de , Sanches Filho Alvino
Published: November 2022

Resumo O trabalho reconstrói a trajetória do Advocacy Coalition Framework (ACF), especialmente seu processo de aprendizado orientado à políticas públicas. Inicialmente, o texto contrasta os elementos contidos em Sabatier e Mazmanian (1980), nos quais são encontrados os primeiros esforços para desenvolver um modelo de análise com os argumentos encontrados em Sabatier (1987, 1988 e 1993). Posteriormente, a trajetória histórica de atualizações e versões do modelo é discutida com base nas análises de Sabatier e Jenkins-Smith (1999), Sabatier e Weible (2007) e Jenkins-Smith et al. (2018). Ao longo de mais de 30 anos de pesquisa, foi possível acompanhar as modificações presentes nas quatro versões do modelo, as principais hipóteses construídas, as críticas e seus desdobramentos Uma lacuna identificada nessa trajetória e consequente proposta para uma futura agenda de pesquisa apontam a influência dos atores internacionais e suas implicações nas modificações de políticas públicas, condição não explicitamente abordada pela ACF em suas diversas versões, conforme destacado por Jenkins-Smith et al. (2018).
Perfil dos secretários municipais de Saúde do Brasil: um panorama de três décadas
Authors: Riquieri Manuella Ribeiro Lira , Carvalho André Luís Bonifácio de , Ouverney Assis Luiz Mafort , Sarti Thiago Dias
Published: November 2022

Resumo Os secretários municipais de saúde são atores estratégicos na construção e governança do SUS. Neste estudo, foi realizada uma análise comparativa do perfil dos secretários municipais ao longo de três décadas (1996, 2006 e 2017), com base em dados de três inquéritos transversais realizados em âmbito nacional, por meio de questionários estruturados direcionados para todos os secretários do país. A análise evidenciou aumento da participação feminina, maior diversidade étnica e racial e maior qualificação profissional dos gestores, além da renovação do perfil geracional, mas ainda com predomínio de homens brancos e importantes diferenças regionais. Foi observado avanço da democratização desses espaços de gestão, mas permanece o desafio de tornar a máquina pública mais representativa do conjunto da sociedade brasileira.
TOTAL HIP ARTHROPLASTY IN THE PUBLIC HEALTH SYSTEM OF SÃO PAULO: COMPARING TYPES OF FIXATION
Authors: GUIMARÃES RODRIGO PEREIRA , VIAMONT-GUERRA MARÍA-ROXANA , ANTONIOLI ELIANE , LENZA MARIO
Published: November 2022

ABSTRACT Brazil lacks registries on the prevalence of primary total hip arthroplasty (THA) fixation methods. Objective: (i) to describe the demographic profile of patients who underwent THA in the public health system of the municipality of São Paulo during the last 12 years and (ii) to compare fixation methods regarding costs, hospital stay length, and death rates. Methods: This is an ecological study conducted with data available on TabNet, a platform belonging to DATASUS. Public data (from the government health system) on THA procedures performed in São Paulo from 2008 to 2019 were extracted. Gender, age, city region, THA fixation method, number of surgeries, costs, hospital stay length, and death rates were analyzed. Results: We analyzed 7,673 THA, of which 6220 (81%) were performed via cementless/hybrid fixation and 1453 (19%), via the cemented technique. Cementless/hybrid fixation had a higher cost (US$ 495.27) than the cemented one (p < 0.001). Nevertheless, hospital stay length was 0.87 days longer for cemented fixation than the cementless/hybrid one. We found no significant difference in death rates between THA fixation methods. Conclusion: THA cementless/hybrid fixation is prevalent in the municipality of São Paulo, which had higher total costs and shorter hospitalizations than cemented fixation. We found no difference between THA fixation methods and death rates. Level of Evidence IV, Case Series.
Fatores associados à não realização do exame clínico das mamas em São Leopoldo, RS, 2015: estudo de base populacional
Authors: Dias-da-Costa Juvenal Soares , Pelinson Andressa , Mattos Cândido Norberto Bronzoni de , Stahnke Douglas Nunes , Donatti Talita , Olinto Maria Teresa Anselmo , Bairros Fernanda
Published: November 2022

Resumo Introdução No Sul do Brasil, a principal causa de mortalidade por neoplasias entre as mulheres está ocupada pelo câncer de mama. O exame clínico anual das mamas está recomendado como medida de rastreamento. Objetivo Investigar a prevalência de não realização do exame clínico de mamas nos últimos 12 meses e fatores associados em mulheres de 20 a 69 anos residentes no município de São Leopoldo/RS em 2015. Método Estudo transversal de base populacional, no qual foram incluídas na análise variáveis demográficas e socioeconômicas. Foram calculadas as razões de prevalência (RP) por regressão de Poisson. Resultados Entre 1.128 mulheres, a prevalência de não realização de exame clínico das mamas foi de 52,6% (IC95%: 51,1-57,2); na análise ajustada, a não realização do exame mostrou-se associada à classe econômica D/E (RP = 1,58; IC95%: 1,16-2,15) e escolaridade de 0 a 4 anos de estudo (RP = 2,16; IC95%: 1,33-3,53). Conclusão As mulheres em situação de vulnerabilidade social apresentaram maior probabilidade de não realização do exame, demonstrando a iniquidade do sistema.
Contribución de la danza contemporánea al bienestar de mujeres maltratadas
Authors: Vera-Esteban María del Carmen , Cardona-Moltó María Cristina
Published: November 2022

Resumen: El presente estudio analiza el estado afectivo-emocional y psicológico de un grupo de mujeres víctimas de violencia machista refugiadas en una casa de acogida y el potencial de la danza contemporánea como recurso de ayuda para su mejora. Las participantes fueron siete mujeres migrantes procedentes de Latinoamérica, Europa del Este y África de edades comprendidas entre 18 y 42 años. Se profundiza en el estudio de un caso para quien se diseña, implementa y evalúa un plan de intervención basado en la danza. Tras su implementación, se observaron cambios a nivel cognitivo (gestión emocional), afectivo (fortalecimiento del autoconcepto y disminución de la ansiedad), y comportamental (ganancias en asertividad y afrontamiento de problemas) que contribuyeron a mejorar su estado de bienestar general. El programa se presenta como un recurso útil de ayuda a mujeres maltratadas y otros colectivos vulnerables.
Sustainability as a perspective for the repowering of small hydroelectric plants in Brazil: estimation method
Authors: Oliveira Marcos André de , Oliveira Márcio Santos Sepúlvia de , Miranda Anna Karolyne Souza
Published: November 2022

Abstract Repowering is a sustainable option for relatively old hydroelectric plants. However, it is still in the process of developing its technical, socio-environmental and regulatory aspects in Brazil. Moreover, there are few papers available in the literature on the subject. A gap in the area is the lack of methodologies that direct the measurement of benefits in suitable enterprises, to prioritize the most opportune ones, reduce risks, and encourage this practice nationally, regionally, and among agents. This paper presents a theoretical discussion of the practices currently employed and proposes an accessible method (simple, fast and low cost) to support decision-making regarding the applicability of repowering in small hydroelectric plants. The results show the environmental sustainability of the practice given the limitations and challenges that currently involve the construction of large hydroelectric plants.
HEALTH-RELATED QUALITY OF LIFE IN PEOPLE WITH CHRONIC WOUNDS AND ASSOCIATED FACTORS
Authors: Dantas Janislei Soares , Silva Ana Cristina de Oliveira e , Augusto Fabiana da Silva , Agra Glenda , Oliveira Jacira dos Santos , Ferreira Lydia Masako , Sawada Namie Okino , Freire Maria Eliane Moreira
Published: November 2022

ABSTRACT Objective to assess health-related quality of life in people with chronic wounds and its association with sociodemographic and health characteristics. Method a cross-sectional and analytical study conducted with 85 individuals with chronic wounds in a university hospital from Paraíba, Brazil. The data were collected from June to September 2019 through interviews guided by the Medical Outcome Study 36-item Short-Form Health Survey generic Quality of Life assessment instruments and the Cardiff Wound Impact Schedule specific tool for people with chronic wounds. The Student's t, ANOVA, Mann-Whitney's U, Kruskal-Wallis and Kolmogorov-Smirnov tests were applied to analyze the data, with 5% significance. Results there was predominance of aged and married women with venous wounds not healing for over 24 weeks. The mean score of the generic instrument, which was 35.08, indicated a negative association between the physical capacity domain and female gender; as well as between vitality and age over 60 years old; between emotional aspects and marital status; of functional capacity, limitation of physical activity and emotional aspects with lack of religious practices; and of functional capacity with worse financial situation, all with p≤0.042. The specific instrument had a mean score of 45.57 and presented negative associations in the physical domain with female gender; as well as between social life without religious practices and social life with worse financial situation, all with p≤0.047. Conclusion the scores of the specific and generic instruments indicated low Quality of Life influenced by factors such as female gender, advanced age, being divorced/separated, lack of religious practices and having financial problems.
QUALIDADE DE VIDA RELACIONADA À SAÚDE DE PESSOAS COM FERIDAS CRÔNICAS E FATORES ASSOCIADOS
Authors: Dantas Janislei Soares , Silva Ana Cristina de Oliveira e , Augusto Fabiana da Silva , Agra Glenda , Oliveira Jacira dos Santos , Ferreira Lydia Masako , Sawada Namie Okino , Freire Maria Eliane Moreira
Published: November 2022

RESUMO Objetivo avaliar a qualidade de vida relacionada à saúde de pessoas com feridas crônicas e sua associação com características sociodemográficas e de saúde. Método estudo transversal e analítico, realizado com 85 pessoas com feridas crônicas de um hospital universitário da Paraíba - Brasil. Os dados foram coletados de junho a setembro de 2019, por meio de entrevistas, norteadas pelos instrumentos de avaliação da qualidade de vida genérico - Medical Outcome Study 36-item Short-Form Health Survey e específico para pessoas com feridas crônicas - Cardiff Wound Impact Schedule. Para analisar os dados, aplicaram-se os testes t de Student, ANOVA, Teste U de Mann-Whitney, Kruskal-Wallis e Kolmogorov-Smirnov, com significância de 5%. Resultados predominaram mulheres idosas, casadas, com feridas venosas que não cicatrizavam há mais de 24 semanas. O escore médio do instrumento genérico, que foi de 35,08, apontou associação negativa entre o domínio capacidade física e o sexo feminino; vitalidade e idade acima de 60 anos; aspectos emocionais e estado civil; capacidade funcional, limitação de atividade física e aspectos emocionais com falta de práticas religiosas; capacidade funcional com pior situação financeira, todos com p≤0,042. O instrumento específico teve escore médio de 45,57 e apresentou associações negativas no domínio físico com o sexo feminino; vida social sem práticas religiosas e vida social com pior situação financeira, todos com p≤0,047. Conclusão as pontuações dos instrumentos específico e genérico indicaram baixa qualidade de vida influenciadas por fatores como sexo feminino, idade avançada, estar divorciado/separado, falta de práticas religiosas e ter problemas financeiros.
O estado da produção do conhecimento científico sobre Dança: um recenseamento em periódicos da Educação Física (1987- 2020)
Authors: Almeida Duilio Queiroz de , Gomes Leonardo do Couto , Medeiros Cristina Carta
Published: November 2022

Resumo O presente estudo objetivou recensear a produção científica de Dança, em formato de artigo, nos periódicos nacionais de Educação Física. Para isso, realizou-se a análise do conteúdo de artigos publicados em 26 periódicos selecionados entre 1987 e agosto de 2020. Encontrou-se 363 artigos no recorte temporal em tela, com enfoques em assuntos, Webqualis e referenciais bibliográficos variados. Pode-se elucidar em que contextos se encontram as produções sobre Dança nacionais, seus destaques e seus principais referenciais bibliográficos. Ao fim, considerou-se que as discussões sobre Dança e aspectos educacionais se mostram predominantes nos achados, assim como o uso de referenciais bibliográficos com certas proximidades teóricas e práticas com atividades dançantes.
The state of scientific knowledge production about Dance: a review in journals of Physical Education (1987-2020)
Authors: Almeida Duilio Queiroz de , Gomes Leonardo do Couto , Medeiros Cristina Carta
Published: November 2022

Abstract The present study aims to register the production of scientific articles about dance in national Physical Education journals. For this, we carried out a content analysis of articles published in 26 selected journals between 1987 and August 2020. We found 363 articles published in the proposed period, focusing on subjects, webqualis, and varied bibliographic references. Thus, it is possible to see where the national dance productions are, their highlights, and their main bibliographic references. In the end, we found that the discussions about dance and educational aspects are predominant, as well as the use of bibliographic references with a certain theoretical and practical proximity to dancing activities.
Passos e descompassos: a dança nos currículos de formação inicial em educação física
Authors: Alencar Allana , Backes Ana Flávia , Manta Sofia Wolker , Resende Rui , Farias Gelcemar Oliveira , Nascimento Juarez Vieira do
Published: November 2022

Resumo O estudo objetiva descrever a oferta de disciplinas na Área da Dança nas estruturas curriculares dos cursos de bacharelado e de licenciatura em Educação Física de universidades federais e estaduais brasileiras. Trata-se de pesquisa de abordagem quantitativa, do tipo documental, que toma como fonte a base de dados “e-MEC” do Ministério da Educação. As evidências revelaram o predomínio da oferta de apenas uma disciplina, de caráter obrigatório, independente do curso e região geográfica. Repensar as estruturas curriculares é recomendado para potencializar a aplicação e ampliar as possibilidades de transposição teórico-prática na Área da Dança na formação inicial em Educação Física.
AIDS as an apparatus: lines, tensions and educations between life, death, health and disease
Authors: Sales Tiago Amaral
Published: November 2022

Abstract This essay reflects on the webs and educations that permeate the body through between life, death, health and disease, focusing on AIDS as an apparatus that interweaves and is interwoven by these multiple facets of existence. We explore cartography as a means of research and draw on authors such as Gilles Deleuze, Michel Foucault, Félix Guattari, Suely Rolnik and Néstor Perlongher, first reflecting on the connections and frictions between life, death, health and illness to then focus on the concept of apparatus and on AIDS as one. Lastly, we address the lines, desires, lusts and tensions that permeate lives affected by the HIV/AIDS epidemic, as well as flight, resistance and minor education.
The impact of universal induction therapy on early hospital readmission of kidney transplant recipients
Authors: Tavares Melissa Gaspar , Cristelli Marina Pontello , Taddeo Julia , Silva Junior Helio Tedesco , Pestana Jose Medina
Published: November 2022

ABSTRACT Background: Early hospital readmission (EHR) is associated with worse outcomes. The use of anti-thymocyte globulin (rATG) induction therapy is associated with increased efficacy in preventing acute rejection, although safety concerns still exist. Methods: This retrospective single-center study compared the incidence, causes of EHR, and one-year clinical outcomes of patients receiving a kidney transplant between August 18, 2011 and December 31, 2012 (old era), in which only high-risk patients received 5 mg/kg rATG, with those transplanted between August 18, 2014 and December 31, 2015 (new era), in which all patients received a single 3 mg/kg dose of rATG. Results: There were 788 patients from the Old Era and 800 from the New Era. The EHR incidence in the old era patients was 26.4% and in the new era patients, 22.5% (p = 0.071). The main cause of EHR in both eras was infection (67% vs. 68%). The incidence of acute rejection episodes was lower (22.7% vs 3.5%, p < 0.001) and the one-year patient survival was higher (95.6% vs. 98.1%, vs. p = 0.004) in new era patients. Conclusion: The universal use of 3 mg/kg rATG single-dose induction therapy in the new era was associated with a trend towards reduced EHR and a reduction in the incidence of acute rejection and mortality.
O impacto da terapia de indução universal na readmissão hospitalar precoce de receptores de transplante renal
Authors: Tavares Melissa Gaspar , Cristelli Marina Pontello , Taddeo Julia , Silva Junior Helio Tedesco , Pestana Jose Medina
Published: November 2022

Resumo Histórico: A Readmissão Hospitalar Precoce (RHP) está associada a piores desfechos. O uso de terapia de indução com globulina antitimócito (rATG, por sua sigla em inglês) está associado ao aumento da eficácia na prevenção de rejeição aguda, embora ainda existam preocupações quanto à segurança. Métodos: Este estudo retrospectivo de centro único comparou a incidência, as causas da RHP e os desfechos clínicos de um ano de pacientes que receberam transplante renal entre 18 de Agosto de 2011 e 31 de Dezembro de 2012 (Antiga Era), em que apenas pacientes de alto risco receberam 5 mg/kg de rATG, com aqueles transplantados entre 18 de Agosto de 2014 e 31 de Dezembro de 2015 (Nova Era), em que todos os pacientes receberam uma única dose de 3 mg/kg de rATG. Resultados: Houve 788 pacientes da Antiga Era e 800 da Nova Era. A incidência de RHP nos pacientes da antiga era foi de 26,4% e nos pacientes da nova era, 22,5% (p = 0,071). A principal causa de RHP em ambas as eras foi infecção (67% vs. 68%). A incidência de episódios de rejeição aguda foi menor (22,7% vs. 3,5%; p < 0,001) e a sobrevida do paciente em um ano foi maior (95,6% vs. 98,1%; vs. p = 0,004) em pacientes da nova era. Conclusão: O uso universal de terapia de indução de 3 mg/kg de rATG em dose única na nova era foi associado a uma tendência à redução da RHP e a uma redução na incidência de rejeição aguda e mortalidade.
Expression Profiles, Prognosis, and ceRNA Regulation of SRY-Related HMG-Box Genes in Stomach Adenocarcinoma
Authors: Zhu Chang , Fu Yuxiang , Xia Ligang , Li Fang , Huang Kaibin , Sun Xiao
Published: November 2022

Aberrant expression of the SRY-related HMG-box (SOX) genes contributes to tumor development and progression. This research aimed to identify the regulation of the SOX genes in stomach adenocarcinoma (STAD). Expression profiles downloaded from The Cancer Genome Atlas (TCGA) were conducted to analyze the expression and function of the SOX genes. A competing endogenous RNAs (ceRNA) network mediated by the SOX genes was effectively constructed consisting of 64 lncRNAs, 29 miRNAs, and 11 SOX genes based on predicted miRNAs shared by lncRNAs and mRNAs using miRDB, TargetScan, miRTarBase, miRcode, and starBase v2.0. SOX9 was identified as a prognostic signature, which showed the usefulness of diagnosis and prognosis of STAD by the receiver operating characteristic (ROC) and Kaplan-Meier curves. SOX9 was also shown specifically in STAD and identified as highly expressed in the gastrointestinal tract. Gene Ontology (GO) enrichment analysis showed that SOX9 might influence the genes related to the pattern specification process, sodium ion homeostasis, and potassium ion transport, mainly including FEZF1, HOXC13, HOXC10, HOXC9, HOXA11, DPP6, ATP4B, CASQ2, KCNA1, ATP4A, and SFRP1. Furthermore, HOTAIR knockdown, miR-206-mimic transfection, the Cell Count Kit-8 (CCK-8) assay were performed to verify the function of HOTAIR/miR-206/SOX9 axis, which was identified in the ceRNA network analysis. HOTAIR could induce proliferation potentially by competitively binding miR-206/SOX9 axis in STAD. These findings provide new clues with prognostic and therapeutic implications in STAD and suggest that HOTAIR/miR-206/SOX9 might be a potential new strategy for therapeutic targeting of gastric cancer.
Covid-19 Salgınının Bölgesel Borsa Turizm Endekslerine Etkilerinin Araştırılması
Authors: TÜMER Tolga , TENGİLİMOĞLU Dilaver
Published: November 2022

Bu çalışmada, bölgesel borsa turizm endekslerinin COVID-19’dan etkilenme düzeyleri araştırılmıştır. Bu amaçla çalışmada, Kuzey Amerika, Avrupa ve Asya Pasifik bölgelerini kapsayan borsa turizm endeksleri kullanılarak istatistiki analizler yapılmıştır. Çalışmanın kapsamı COVID-19’un dünyaya yayılmaya başladığı dönemden itibaren 22 Ocak 2020-22 Ocak 2022 tarihleri arasındaki iki yıllık süreci kapsamaktadır ve çalışmada bu süreç çeyrek dilimlere ayrılarak sekiz dönem kapsamında analiz edilmektedir. Günlük veriler kullanılan araştırmada hem dünyadaki hem de kullanılan endekslerin kapsadığı bölgelerdeki COVID-19 verileri dikkate alınmıştır. COVID-19 verileri olarak günlük vaka, günlük ölüm, toplam vaka ve toplam ölüm sayıları toplanmıştır. Araştırmanın sonucunda, borsa turizm endekslerini negatif yönde en fazla COVID-19 kaynaklı bölgesel günlük ölümlerin etkilediği bulunmuştur. Bu çalışma, geniş kapsamı, bölgesel ve dönemsel karşılaştırma sunan bulguları ile alanyazına katkı sunmaktadır.
Promotive role of USP29-mediated deubiquitination in malignant proliferation of colorectal cancer cells via the KIAA1429/SOX8 axis
Authors: Li Juncai , Yang Jianguo , Chen Zhenzhou , Liu Li , Wang Hao , Deng Qican , Chen Yajun , Que Yong , Fu Zhongxue
Published: November 2022

Colorectal cancer (CRC) is regarded as one of the most prevalent neoplasms worldwide, and ubiquitination and N6-methyladenosine (m6A) modification regulate the outgrowth of multiple cancers. This study attempted to explore the effect of ubiquitin-specific peptidases 29 (USP29) on the malignant proliferation of CRC cells via stabilizing Vir-like m6A methyltransferase associated (VIRMA/KIAA1429). First, upregulations of USP29, KIAA1429, and SRY-box transcription factor 8 (SOX8) were found in CRC tissues and cells through real-time quantitative polymerase chain reaction and Western blotting. After transfection of si-USP29, the proliferation of CRC cells was evaluated by the cell counting kit-8, colony formation and 5-ethynyl-2’-deoxyuridine assays, and we observed that depletion of USP29 inhibited the proliferation of CRC cells. Co-immunoprecipitation confirmed the binding of USP29 to KIAA1429. Mechanically, USP29 mediated deubiquitination to stabilize the protein levels of KIAA1429, and KIAA1429 promoted the stability of SOX8 mRNA through m6A modification. Moreover, overexpression of KIAA1429 or SOX8 reversed the inhibitory effects of USP29 depletion on CRC cell proliferation. Finally, the xenograft tumor model revealed the promotive role of USP29 in the proliferation of CRC cells in vivo. Altogether, USP29 facilitates the malignant proliferation of CRC cells via upregulating the KIAA1429/SOX8 axis.
Germination and seedling establishment for deep-flow hydroponics: The benefit of slant boards v2
Authors: not provided noah.langenfeld
Published: November 2022

Germination and seedling establishment for transplanting into hydroponics often uses porous substrates, but fine roots grow into these substrates, and they cannot be removed without damaging roots. Seedlings transplanted without removal of substrates can cause interactions with solution chemistry or addition of particulates to the nutrient solution. Germination of seeds on slant boards is clean, uniform, and reduces the time to transplanting. Slant boards facilitate long roots, which maximize exposure of the primary root to the nutrient solution after transplanting. The “boards” are made from thin acrylic or polycarbonate sheets with germination paper on top. Seeds are held in place by covering with thin paper before vertical placement of the boards in a container. Four to twelve days later, the seedlings with long roots can be removed from the paper without damage and transplanted into the hydroponic system. Here we describe slant board construction and procedures for rapid germination and transplanting. (The last step contains a supplemental video with extra context and tips, as part of the protocols.io Spotlight series, featuring conversations with protocol authors.)
A Review of Forecasting Studies for the Hotel Industry: Focusing on results, contributions and limitations
Authors:   People&Global Business Association, Choi Jeong-Gil , Zhang Yi-Wei , Nadzri Nurulain Izzati Binti Mohd , Baymuminova Nigora , Xu Shi-Nyu
Published: November 2022

Purpose: The purpose of this study is to conduct a thorough review of the results, contributions, and limitations of forecasting studies in the hotel industry to uncover how those studies contribute to the body of knowledge and what limitations of those studies should be filled by future studies.
Design/methodology/approach: To uncover the overall trends and evolution of hotel forecasting findings, this study used a systematic literature review approach to ensure the strictness and transparency of the review process. To select a paper to be reviewed, two main review processes including keyword searching and citation counts of each paper were conducted. To assure the rigor of our first two procedures, this study took further steps including cross-checking the results of those studies and calculating the average citation per year of the selected studies. The selected papers were analyzed based upon their forecasting areas, the contribution of each paper by using citation score, frequency of using methodology or techniques, demographic characteristics of authors, and major trends of the forecasting studies.
Findings: This research has compiled a list of the most cited papers, as well as the average number of citations per year, the top list of the most used nations and subject areas which manifested the contribution to the body of literature in this study.
Research limitations/implications: Future research should consider using other platforms such as Web of Science (WoS) or Scopus to emphasize more the impact and value of the literature study. The outcome of this study will help other researchers to have a better understanding of the used models in forecasting the industry as well as to decipher the most and the least studied research areas in the forecasting hotel industry.
Originality/value: This study explored the overall development, contributions, and limitations of forecasting studies in the hospitality industry in an updated, thorough literature analysis. The themes and types of research that have already been studied can be seen by the researchers, as well as those that have not yet been explored.
Basics of professional communication in general practice
Authors: Degtyareva Ludmila N.
Published: November 2022

Knowledge and skills in the field of professional communication are necessary for the doctor to form a trusting relationship with the patient. The right approach to counseling based on this knowledge is important to ensure quality medical care. The consultation has two main tasks: to find out the essence of the problem that led the patient to the doctor, and to find possible solutions to this problem. At the same time, a combination of openness and structuring of the process is important.
The lecture examines the Calgary-Cambridge model of counseling, its structure and features of each stage. The structure of the consultation in accordance with this model includes five stages the beginning of the consultation, the collection of information, inspection, explanation and planning, and the completion of the reception. Each stage has certain tasks, starting with establishing contact at the beginning of the consultation, and ending with summing up at the end of the reception.
The principles and conditions of effective counseling include the availability of sufficient time; availability of consultation; respect for the personality of each patient and goodwill; confidentiality; provision of information in a language understandable to the patient; respect for the patients ability to make decisions.
Communication with a patient in the conditions of primary health care is a long and multifaceted process, but the result of such communication often depends on the successful conduct of counseling by a doctor.
Blind Eye An Investigation of Accessible Art Content within Arts Organizations that Does Not Shy Away from the Blind and Visually Impaired
Authors:   , Godin Angela RaeGéne , Coleman Laura-Edythe , Zitcer Andrew
Published: November 2022

Blind Eye focuses on the need for accessibility within artistic content that services the blind and visually impaired, which is essential for promoting equitable inclusion in the 21st century. Adequate, conscious programming developed as accessible content can provide a gateway for this population to become a robust presence in the sector as artists or arts administrators. The phenomenological research is studied through semi-structured interviews to understand the accessibility of program content produced by arts organizations for blind and visually impaired artists and blind and visually impaired patrons. The literature review discusses the lack of universal accessible artistic content, while divulging what tangible artistic programing does exist for this population. The literature explores the historical effects of accessible art programming that caters to the blind and visually impaired through multiple case studies, scholarly research, and practical real-life examples. Additionally, the blind and visually impaired artist interview findings examine their personal experiences through their art and correlates to arts organizations that provide accessible artistic programs. The findings explore the successes and limitations of accessible artistic programs and point out initiatives that can be put into place by arts organizations not currently serving this underrepresented population. Nevertheless, the overarching takeaway is for the arts and culture sector to take a drastic step toward artistic content becoming accessible for the blind and visually impaired community, resulting in long-term change. (Blind Eye is presented in a conversational tone to enable the largest, most diverse percentage of the population to be able to utilize and comprehend its purpose and findings.)
Power Quality Control for Mixed Renewable Energy Systems
Authors: Akkilah Mohammed , Farsadi Murtaza
Published: November 2022

Electrical energy is one of the famous and easiest used forms of energy. Electrical energy can be converted to many forms of energy. With the development of technology, the dependency on the electrical energy has been increased greatly nowadays. Such as Computer and telecommunication networks field, railway network banking, post office, life support system are few application that just cannot run without electricity. In this article we will discuss the power quality issues for distributed generation systems based on renewable energy sources, as an example solar and wind energy. A brief explanation about the power quality issues is conducted here.This article starts with the power quality issues, followed by discussions of basic standards. A brief study of power quality in the power system, it includes the systems with dc and renewable sources are done in this article. In addition to the power quality monitoring techniques and its possible solutions for the power quality issues for the power system are briefly discussed. Later we will go through the analyzing step for the methods of mitigation of these problems using custom power devices, such as D-STATCOM, UPQC, UPS, TVSS, DVR, etc., for micro grid systems. As an example for the renewable energy systems, such as the statcom can be an efficient choice due to its several advantages, whereas the spinning reserve can enhance the power quality in traditional systems. Finally, we will discuss the power quality in dc systems. Two Main advantages for a DC system, is the Simple arrangements and higher reliability. Even though it faces many power quality issues such as, instability and poor detection of faults.
A Control Method for Modified Bidirectional Cuk DC-DC Converter
Authors: Azmi Muhammad Rifqi , Dahono Andriazis , Ilman Sofyan M. , Rizqiawan Arwindra , Dahono Pekik A.
Published: November 2022

A control method for a modified bidirectional Cuk DC-DC converter is proposed in this paper. The proposed controller is a double control loop consisting of an inner current control loop and a voltage control loop is used to control the converter. The small-signals model that is useful in designing controllers is derived in this paper. A simple PI controller is used for both controllers. The simulation and experimental results are included in this paper to validate the performance of the proposed controller.
Design and Construction of Electric Socket Based Photovoltaic
Authors: Widadiy Mohammad Fakhim , Afandi A.N. , Hiyama T.
Published: November 2022

Human needs for energy are increasing but the level of fossil energy is getting depleted, making energy sources dwindling. alternative energy or renewable energy is needed. Indonesia has a lot of solar energy potential as much as 4.8 KWh/m2, this amount is equal to 112,000 GWp, but only about 10 MWp has been used. To maximize the potential of solar power, researchers carry out designs aimed at maximizing the potential of solar power, especially providing energy at shelters so that they have an independent energy supply and are not dependent on energy sourced from conventional energy. From the results of observations related to the electrical design of the socket at the bus stop with a photovoltaic basis, it was found that the total load affects the use of PLTS components, so it is necessary to determine the load first before the design process.
Design of New Renewable Energy Utilization Technology in Islamic Boarding Schools
Authors: Hartawan Hartawan , Kurniawan Fachrul , Haris H.R. Abdul , Wibawa Aji Prasetya , Durai Akshaya
Published: November 2022

Applications of solar power that are very suitable to be developed in Islamic boarding schools are lighting and activities that have recently been made extra-curricular activities and economic recovery by farming using hydroponic technology. The results of the research on solar street lighting on each component function properly according to their respective specifications and the proximity sensor detects the presence of objects within 0.5 meters, 1 meter and 1.5 meters from the sensor until the buzzer sounds for a few seconds. Furthermore, research on solar hydroponic plants using ac motors in testing ac motors using inverters has a weakness if the inverter is used for 24 hours it will reduce the life of the inverter or the inverter will be damaged faster, In addition, choosing an inverter that does not match the specifications of the motor will also have an impact on the motor itself, in this case it is necessary to pay attention to the selection of the inverter to be used. However, if you use two motors at the same time, it will reduce the impact of damage more quickly on components because the motors work alternately by setting the programmble DC timer. In both solar power applications, Based on the results of the investment analysis, the costs are quite cheap because they reduce the bill for payment each month and can return the capital for making the application. if you use two motors at the same time it will reduce the impact of damage more quickly on the components because the motors work alternately by setting the programmble DC timer. In both solar power applications, based on the results of the investment analysis, the costs are quite cheap because they reduce the bill for payment each month and can return the capital for making the application. if you use two motors at the same time it will reduce the impact of damage more quickly on the components because the motors work alternately by setting the programmble DC timer. In both solar power applications, based on the results of the investment analysis, the costs are quite cheap because they reduce the bill for payment each month and can return the capital for making the application.
Hybrid Method for Optimization of Permanent Magnet Synchronous Machine (PMSM) Rotation using FA-ANFIS
Authors: Haikal Muhammad Agil , Ali Machrus , Budi S Irrine , Mohd Noh Faridah Hanim Binti
Published: November 2022

PMSM uses the principle of Faraday's experiment by rotating a magnet in a coil by utilizing another energy source. When the magnet moves in the coil or vice versa. The rotation of the machine will change the flux of magnetic force on the coil and penetrate perpendicularly to the coil so that a potential difference arises between the ends of the coil. It is caused by a change in magnetic flux. The Firefly Algorithm (FA) method has been proven successful in overcoming system optimization problems. Modifying the FA is expected to improve its performance of the FA. To get the best control method, it is necessary to vary the speed control model. This study compares the PMSM speed control without a controller, PID Control, PID-FA, and PID-FA-ANFIS. The simulation results show that the best model on the PID-FA-ANFIS controller which is closest to the Speed Reff (2980 rpm) is that PID-FA-ANFIS obtains a rotation profile with the smallest undershot, the fastest steady state, the best output current profile, the best torque profile, and the best stress profile. The results of this study will be followed by other uses of artificial intelligence.