bluuebunny/crossref_metadata_2025 huggingface dataset:
Understanding the Role of Autophagy in Cancer Formation and Progression Is a Real Opportunity to Treat and Cure Human Cancers
Authors: Patergnani Simone , Missiroli Sonia , Morciano Giampaolo , Perrone Mariasole , Mantovani Cristina M. , Anania Gabriele , Fiorica Francesco , Pinton Paolo , Giorgi Carlotta
Published: November 2021

The malignant transformation of a cell produces the accumulation of several cellular adaptions. These changes determine variations in biological processes that are necessary for a cancerous cell to survive during stressful conditions. Autophagy is the main nutrient recycling and metabolic adaptor mechanism in eukaryotic cells, represents a continuous source of energy and biomolecules, and is fundamental to preserve the correct cellular homeostasis during unfavorable conditions. In recent decades, several findings demonstrate a close relationship between autophagy, malignant transformation, and cancer progression. The evidence suggests that autophagy in the cancer context has a bipolar role (it may act as a tumor suppressor and as a mechanism of cell survival for established tumors) and demonstrates that the targeting of autophagy may represent novel therapeutic opportunities. Accordingly, the modulation of autophagy has important clinical benefits in patients affected by diverse cancer types. Currently, about 30 clinical trials are actively investigating the efficacy of autophagy modulators to enhance the efficacy of cytotoxic chemotherapy treatments. A deeper understanding of the molecular pathways regulating autophagy in the cancer context will provide new ways to target autophagy for improving the therapeutic benefits. Herein, we describe how autophagy participates during malignant transformation and cancer progression, and we report the ultimate efforts to translate this knowledge into specific therapeutic approaches to treat and cure human cancers.
In Silico Identification and Clinical Validation of a Novel Long Non-Coding RNA/mRNA/miRNA Molecular Network for Potential Biomarkers for Discriminating SARS CoV-2 Infection Severity
Authors: Agwa Sara H. A. , Elghazaly Hesham , Meteini Mahmoud Shawky El , Shawky Sherif M. , Ali Marwa , Abd Elsamee Aya M. , Sayed Safa Matbouly , Sherif Nadine , Sharaf Howida M. , Alhadidy Mohamed A. , Matboli Marwa
Published: November 2021

(1) Background: The coronavirus (COVID-19) pandemic is still a major global health problem, despite the development of several vaccines and diagnostic assays. Moreover, the broad symptoms, from none to severe pneumonia, and the various responses to vaccines and the assays, make infection control challenging. Therefore, there is an urgent need to develop non-invasive biomarkers to quickly determine the infection severity. Circulating RNAs have been proven to be potential biomarkers for a variety of diseases, including infectious ones. This study aimed to develop a genetic network related to cytokines, with clinical validation for early infection severity prediction. (2) Methods: Extensive analyses of in silico data have established a novel IL11RA molecular network (IL11RNA mRNA, LncRNAs RP11-773H22.4 and hsa-miR-4257). We used different databases to confirm its validity. The differential expression within the retrieved network was clinically validated using quantitative RT-PCR, along with routine assessment diagnostic markers (CRP, LDH, D-dimmer, procalcitonin, Ferritin), in100 infected subjects (mild and severe cases) and 100 healthy volunteers. (3) Results: IL11RNA mRNA and LncRNA RP11-773H22.4, and the IL11RA protein, were significantly upregulated, and there was concomitant downregulation of hsa-miR-4257, in infected patients, compared to the healthy controls, in concordance with the infection severity. (4) Conclusion: The in-silico data and clinical validation led to the identification of a potential RNA/protein signature network for novel predictive biomarkers, which is in agreement with ferritin and procalcitonin for determination of COVID-19 severity.
The New Kid on the Block: HLA-C, a Key Regulator of Natural Killer Cells in Viral Immunity
Authors: Vollmers Sarah , Lobermeyer Annabelle , Körner Christian
Published: November 2021

The human leukocyte antigen system (HLA) is a cluster of highly polymorphic genes essential for the proper function of the immune system, and it has been associated with a wide range of diseases. HLA class I molecules present intracellular host- and pathogen-derived peptides to effector cells of the immune system, inducing immune tolerance in healthy conditions or triggering effective immune responses in pathological situations. HLA-C is the most recently evolved HLA class I molecule, only present in humans and great apes. Differentiating from its older siblings, HLA-A and HLA-B, HLA-C exhibits distinctive features in its expression and interaction partners. HLA-C serves as a natural ligand for multiple members of the killer-cell immunoglobulin-like receptor (KIR) family, which are predominately expressed by natural killer (NK) cells. NK cells are crucial for the early control of viral infections and accumulating evidence indicates that interactions between HLA-C and its respective KIR receptors determine the outcome and progression of viral infections. In this review, we focus on the unique role of HLA-C in regulating NK cell functions and its consequences in the setting of viral infections.
Application of the Pluripotent Stem Cells and Genomics in Cardiovascular Research—What We Have Learnt and Not Learnt until Now
Authors: Simeon Michael , Dangwal Seema , Sachinidis Agapios , Doss Michael
Published: November 2021

Personalized regenerative medicine and biomedical research have been galvanized and revolutionized by human pluripotent stem cells in combination with recent advances in genomics, artificial intelligence, and genome engineering. More recently, we have witnessed the unprecedented breakthrough life-saving translation of mRNA-based vaccines for COVID-19 to contain the global pandemic and the investment in billions of US dollars in space exploration projects and the blooming space-tourism industry fueled by the latest reusable space vessels. Now, it is time to examine where the translation of pluripotent stem cell research stands currently, which has been touted for more than the last two decades to cure and treat millions of patients with severe debilitating degenerative diseases and tissue injuries. This review attempts to highlight the accomplishments of pluripotent stem cell research together with cutting-edge genomics and genome editing tools and, also, the promises that have still not been transformed into clinical applications, with cardiovascular research as a case example. This review also brings to our attention the scientific and socioeconomic challenges that need to be effectively addressed to see the full potential of pluripotent stem cells at the clinical bedside.
Antenatal Diagnosis of a Partial Atrioventricular Canal with Ebstein’s Anomaly
Authors: Laforest Gerald , Selly Jean-Bernard , Dubois Gilbert , Kreitmann Bernard , Levy Yael
Published: November 2021

The simultaneous occurrence of an atrioventricular canal defect (AVCD) and Ebstein’s anomaly is extremely rare, occurring in less than 0.5% of all patients with AVCD. Only 22 cases are described in the literature. This patient’s antenatal diagnosis of both Ebstein’s anomaly and partial AVCD was made at 25 weeks of gestation. The delivery was organized in a tertiary center. The initial neonatal course was difficult but with adequate treatment, a rapid improvement allowed for a gap of almost 2 years before a complete surgical repair including a cone tricuspid plasty. To our knowledge, this is the first case of antenatal diagnosis, with carefully tailored delivery, neonatal care and subsequent follow-up before indication for successful surgery.
Pediatric Oncology Palliative Care Programs in Central America: Pathways to Success
Authors: Gómez García Wendy Cristhyna , Rivas Silvia , Paz Gabriela , Bustamante Marisol , Castro Gerardo , Gutiérrez Hazel , Ah Chu Maria Sabina , Gassant Pascale Yola , Larin Lovo Rolando , Gamboa Yessika , Torres Núñez Marleni , García Quintero Ximena , Okhuysen-Cawley Regina
Published: November 2021

Palliative care offers children who have life-limiting and life-threatening oncologic illnesses and their families improved quality of life. In some instances, impeccable symptom control can lead to improved survival. Cultural and financial barriers to palliative care in oncology patients occur in all countries, and those located in Central America are no exception. In this article, we summarize how the programs participating in the Asociación de Hemato-Oncólogos Pediatras de Centro America (AHOPCA) have developed dedicated oncology palliative care programs. The experience in Guatemala, El Salvador, Costa Rica, Panama, Dominican Republic and Haiti is detailed, with a focus on history, the barriers that have impeded progress, and achievements. Future directions, which, of course, may be impacted by the COVID-19 pandemic, are described as well.
Morphological and Structural Evolution of Chemically Deposited Epitaxially LaNiO3 Thin Films
Authors: Nasui Mircea , Sonher Ramona Bianca , Ware Ecaterina , Daniel Andrada , Petrisor Traian , Gabor Mihai Sebastian , Ciontea Lelia , Petrisor Traian
Published: November 2021

We report the preparation and characterization of epitaxial LaNiO3 (LNO) thin films by chemical solution deposition method using lanthanum and nickel acetylacetonates as starting reagents dissolved in propionic acid. In order to obtain further information regarding the decomposition behavior of the film, the precursor solution was dried to obtain the precursor powder, which was investigated by thermal analyses and X-ray diffraction measurements (XRD). The LNO perovskite thin films were deposited by spin coating on SrTiO3(100) single crystal substrates. A detailed study with different crystallization temperatures (600–900 °C) at two different heating ramps (5 and 10 °C/min) was performed. Oriented LaNiO3 thin films with good out-of-plane textures were obtained with optimal surface morphologies.
Seagrass Patch Complexity Affects Macroinfaunal Community Structure in Intertidal Areas: An In Situ Experiment Using Seagrass Mimics
Authors: Brun Fernando G. , Cobo-Díaz José F. , González-Ortiz Vanessa , Varela José L. , Pérez-Lloréns José Lucas , Vergara Juan J.
Published: November 2021

Seagrasses, as key ecosystem engineers in coastal ecosystems, contribute to enhancing diversity in comparison with nearby bare areas. It has been proved mainly for epifauna, but data on infauna are still scarce. The present study addresses how seagrass structural complexity (i.e., canopy properties) affects the diversity of infaunal organisms inhabiting those meadows. Canopy attributes were achieved using seagrass mimics, which were used to construct in situ vegetation patches with two contrasting canopy properties (i.e., shoot density and morphology) resembling the two seagrass species thriving in the inner Cadiz Bay: Zostera noltei and Cymodocea nodosa. After three months, bare nearby areas, two mimicked seagrass patches (‘Zostera’ and ‘Cymodocea’), and the surrounding natural populations of Zostera noltei were sampled in a spatially explicit way. Shifts in organism diets were also determined using 15N and 13C analyses in available food sources and main infaunal organisms, mixing models, and niche metrics (standard ellipse area). Seagrass-mimicked habitats increased the species richness (two-fold), organism abundance (three to four times), and functional diversity compared with bare nearby areas. The clam Scrobicularia plana (deposit/filter feeder) and the worm Hediste diversicolor (omnivore) were dominant in all of the samples (> 85%) and showed an opposite spatial distribution in the reconstructed patches: whilst S. plana accumulated in the outer-edge parts of the meadow, H. diversicolor abounded in the center. Changes in the isotopic signature of both species depending on the treatment suggest that this faunal distribution was associated with a shift in the diet of the organisms. Based on our results, we concluded that facilitation processes (e.g., reduction in predation and in bioturbation pressures) and changes in food availability (quality and quantity) mediated by seagrass canopies were the main driving forces structuring this community in an intertidal muddy area of low diversity.
Event-Triggered Formation Tracking Control for Unmanned Aerial Vehicles Subjected to Deception Attacks
Authors: Sun Biao , Gu Zhou , Xiong Tianyi
Published: November 2021

This study investigates the time-varying formation tracking (TVFT) control problem for multiple unmanned aerial vehicle (multi-UAV) systems under deception attacks by utilizing an event-triggered mechanism (ETM). First, for the sake of alleviating the communication burden, an effective ETM is designed in this paper. Second, to deal with deception attacks in the communication network, a random deception attack model under the designed ETM is constructed. Finally, a novel formation tracking control scheme for multi-UAV systems under deception attack combining the ETM is proposed to achieve the expected TVFT. The stability analysis of the formation control system is given by using the Lyapunov stability theory and linear matrix inequality (LMI) technique. Simulations are conducted to verify the effectiveness of the proposed formation control scheme.
Development of Korean Indigenous Low-Temperature-Tolerant Yeast for Enhancing the Flavor of Yakju
Authors: Park Jong-Beom , Choi Jun-Su , Park Hye-Won , Lee Sae-Byuk , Park Heui-Dong
Published: November 2021

Yakju, a traditional fermented beverage in Korea, is prepared using various raw materials and methods, and, hence, exhibits various characteristics. Low-temperature-fermented yakju can inhibit the growth of undesirable bacteria and is known for its unique flavor and refreshing taste. To increase the production of volatile aromatic compounds in yakju, strains with strong resistance to low temperatures and excellent production of volatile aromatic compounds were screened from indigenous fruits (grape, persimmon, plum, aronia, wild grape) and nuruk in Korea. One Saccharomyces cerevisiae and three non-Saccharomyces strains were finally screened, and yakju was fermented at 15 °C through mono/co-culture. The analysis of volatile aromatic compounds showed that S. cerevisiae W153 produced 1.5 times more isoamyl alcohol than the control strain and reduced the production of 2,3-butanediol by a third. Similarly, a single culture of Pichia kudriavzevii N373 also produced 237.7 mg/L of ethyl acetate, whereas Hanseniaspora vineae G818 produced ~11 times greater levels of 2-phenethyl acetate than the control. Alternatively, Wickerhamomyces anomalus A159 produced 95.88 mg/L of ethyl hexadecanoate. During principal component analysis, we also observed that the co-culture sample exhibited characteristics of both volatile aroma compounds of the single cultured sample of each strain. Our results suggest that yakju with unique properties can be prepared using various non-Saccharomyces strains.
An Arbitrary Hybrid Turbulence Modeling Approach for Efficient and Accurate Automotive Aerodynamic Analysis and Design Optimization
Authors: Maulenkul Saule , Yerzhanov Kaiyrbek , Kabidollayev Azamat , Kamalov Bagdaulet , Batay Sagidolla , Zhao Yong , Wei Dongming
Published: November 2021

The demand in solving complex turbulent fluid flows has been growing rapidly in the automotive industry for the last decade as engineers strive to design better vehicles to improve drag coefficients, noise levels and drivability. This paper presents the implementation of an arbitrary hybrid turbulence modeling (AHTM) approach in OpenFOAM for the efficient simulation of common automotive aerodynamics with unsteady turbulent separated flows such as the Kelvin–Helmholtz effect, which can also be used as an efficient part of aerodynamic design optimization (ADO) tools. This AHTM approach is based on the concept of Very Large Eddy Simulation (VLES), which can arbitrarily combine RANS, URANS, LES and DNS turbulence models in a single flow field depending on the local mesh refinement. As a result, the design engineer can take advantage of this unique and highly flexible approach to tailor his grid according to his design and resolution requirements in different areas of the flow field over the car body without sacrificing accuracy and efficiency at the same time. This paper presents the details of the implementation and careful validation of the AHTM method using the standard benchmark case of the Ahmed body, in comparison with some other existing models, such as RANS, URANS, DES and LES, which shows VLES to be the most accurate among the five examined. Furthermore, the results of this study demonstrate that the AHTM approach has the flexibility, efficiency and accuracy to be integrated with ADO tools for engineering design in the automotive industry. The approach can also be used for the detailed study of highly complex turbulent phenomena such as the Kelvin–Helmholtz instability commonly found in automotive aerodynamics. Currently, the AHTM implementation is being integrated with the DAFoam for gradient-based multi-point ADO using an efficient adjoint solver based on a Sparse Nonlinear optimizer (SNOPT).
Impact of Nutritional Intervention on Taste Perception—A Scoping Review
Authors: Micarelli Alessandro , Malacrida Sandro , Strapazzon Giacomo , Mrakic-Sposta Simona , Micarelli Beatrice , Alessandrini Nicolò , Carbini Valentina , Caputo Sara , Falla Marika , Alessandrini Marco
Published: November 2021

The aim of the present scoping review was to evaluate the impact of experimental meal loads or observational diet changes/habits on taste tests in both healthy subjects and patients. A systematic search performed in PubMed, Scopus, and Institute for Scientific Information (ISI) Web of Science electronic databases retrieved, respectively 2981, 6258, and 7555 articles from January 2000 to December 2020. A total of 17 articles were included for full-text review. Literature results were stratified according to the observational/interventional approach, the involvement of healthy subjects or patients, the taste test, and the meal/dietary changes. The present scoping review reinforced the notions postulating that certain taste tests (for example focusing on fatty acid, salt, or sugar) might be specifically influenced by the nutritional intervention and that other ones might be susceptible to a wide span of changes beyond the extent of tastant included in the specific food changes. This could also depend on the inhomogeneity of literature trend: The short duration of the intervention or the random type of meal load, unsuitability of the taste test chosen, and the presence of underlying disorders. Future studies for a better comprehension of taste tests reliability in relation to specific food changes are thus to be fostered.
Impact of the Freeze-Drying Conditions Applied to Obtain an Orange Snack on Energy Consumption
Authors: Silva-Espinoza Marilú Andrea , Camacho María del Mar , Martínez-Monzó Javier , Martínez-Navarrete Nuria
Published: November 2021

Nowadays, the consumer is looking for healthier, more attractive, ready-to-eat, and safer foodstuffs than fresh products. Despite freeze drying being known for providing high added value products, it is a slow process which is conducted at low pressures, so, in terms of energy consumption, it turns out to be quite costly for the food industry. With the purpose of obtaining a freeze-dried orange puree, previously formulated with gum Arabic and bamboo fiber, which can be offered to consumers as a snack at a low economic cost, the impact of the process conditions on energy consumption has been considered. The product temperature evolution and the energy consumption were registered during the drying of frozen samples at different combinations of chamber pressures (5 and 100 Pa) and shelf temperatures (30, 40 and 50 °C). In each case, the time processing was adapted in order to obtain a product with a water content under 5 g water/100 g product. In this study, the secondary drying stage was considered to start when the product reached the shelf temperature and both the pressure and the temperature affected the duration of primary and secondary drying stages. The results obtained led to the conclusion that the shorter duration of the process when working at 50 °C results in significant energy saving. Working at a lower pressure also contributes to a shortening of the drying time, thus reducing the energy consumption: the lower the temperature, the more marked the effect of the pressure.
The Prevalence and Epidemiology of Salmonella in Retail Raw Poultry Meat in China: A Systematic Review and Meta-Analysis
Authors: Sun Tianmei , Liu Yangtai , Qin Xiaojie , Aspridou Zafeiro , Zheng Jiaming , Wang Xiang , Li Zhuosi , Dong Qingli
Published: November 2021

Foodborne disease caused by Salmonella is an important public health concern worldwide. Animal-based food, especially poultry meat, is the main source of human salmonellosis. The objective of this study was to evaluate the prevalence and epidemiology of Salmonella contamination in raw poultry meat commercialized in China. Following the principle of systematic review, 98 sets of prevalence data were extracted from 74 publications conducted in 21 Chinese provincial regions. The random-effect model was constructed for subgrouping analysis by meat category, preservation type, and geographical location. The prevalence levels differed from high to low among raw poultry meat, including chicken, 26.4% (95% CI: 22.4–30.8%); pigeon, 22.6% (95% CI: 18.2–27.8%); duck, 10.1% (95% CI: 5.3–18.2%); and other poultry meat, 15.4% (95% CI: 12.0–19.5%). Prevalence data on the preservation type revealed that chilled poultry meat might be more likely to experience cross-contamination than non-chilled poultry meat in China. The distribution map of Salmonella for raw poultry meat showed that a higher prevalence level was found in the Shaanxi, Henan, Sichuan, and Beijing regions. All subgroups possessed high amounts of heterogeneity (I2 > 75%). The scientific data regarding the differences in prevalence levels between meat category, preservation method, and geographical region sources might be useful to improve specific interventions to effectively control the incidence of Salmonella in poultry meat.
Impact of G-Quadruplexes and Chronic Inflammation on Genome Instability: Additive Effects during Carcinogenesis
Authors: Stein MaryElizabeth , Eckert Kristin A.
Published: November 2021

Genome instability is an enabling characteristic of cancer, essential for cancer cell evolution. Hotspots of genome instability, from small-scale point mutations to large-scale structural variants, are associated with sequences that potentially form non-B DNA structures. G-quadruplex (G4) forming motifs are enriched at structural variant endpoints in cancer genomes. Chronic inflammation is a physiological state underlying cancer development, and oxidative DNA damage is commonly invoked to explain how inflammation promotes genome instability. We summarize where G4s and oxidative stress overlap, with a focus on DNA replication. Guanine has low ionization potential, making G4s vulnerable to oxidative damage. Impacts to G4 structure are dependent upon lesion type, location, and G4 conformation. Occasionally, G4s pose a challenge to replicative DNA polymerases, requiring specialized DNA polymerases to maintain genome stability. Therefore, chronic inflammation creates a dual challenge for DNA polymerases to maintain genome stability: faithful G4 synthesis and bypassing unrepaired oxidative lesions. Inflammation is also accompanied by global transcriptome changes that may impact mutagenesis. Several studies suggest a regulatory role for G4s within cancer- and inflammatory-related gene promoters. We discuss the extent to which inflammation could influence gene regulation by G4s, thereby impacting genome instability, and highlight key areas for new investigation.
Insights into the Evolution of Spermatogenesis-Related Ubiquitin–Proteasome System Genes in Abdominal Testicular Laurasiatherians
Authors: Ding Xiaoyue , Cao Li , Zheng Yu , Zhou Xu , He Xiaofang , Xu Shixia , Ren Wenhua
Published: November 2021

During embryonic development in mammals, the testicles generally descend into the scrotum, making the testicular temperature 2–4 °C lower than the core temperature via heat exchange and clearance, and thus more beneficial for normal spermatogenesis. Failure to descend, known as cryptorchidism, carries a series of risks such as infertility and testicular cancer. However, some mammals have evolved abdominal testes while maintaining healthy reproduction. To explore the underlying molecular mechanism, we conducted comparative genomic analyses and functional assays on the spermatogenesis-related ubiquitin–proteasome system (UPS) genes essential to sperm formation in representative laurasiatherians. Here, positive selection and rapid evolution of spermatogenesis-related UPS genes were identified in the abdominal testicular laurasiatherians. Moreover, potential convergent amino acids were found between distantly related species with similar abdominal testicles and functional analyses showed RNF8 (V437I) in abdominal testicular species (437I) has a stronger ubiquitination ability, which suggests that the mammals with abdominal testes might exhibit enhanced sperm cell histone clearance to maintain sperm formation. This evidence implies that, in response to “cryptorchidism injury”, spermatogenesis-related UPS genes in the abdominal testicular species might have undergone adaptive evolution to stabilize sperm formation. Thus, our study could provide some novel insights into the reproductive adaptation in abdominal testicular mammals.
Identification of Susceptibility Genes for Fusarium oxysporum in Cucumber via Comparative Proteomic Analysis
Authors: Xu Jun , Wang Ke , Xian Qianqian , Zhang Ningyuan , Dong Jingping , Chen Xuehao
Published: November 2021

Fusarium wilt (FW) in cucumber (Cucumis sativus L.), caused by Fusarium oxysporum f. sp. cucumerinum (Foc), poses a major threat to cucumber growth and productivity. However, lack of available natural resistance resources for FW restricts the breeding of resistant cultivars via conventional approaches. Susceptibility (S) genes in susceptible host plants facilitate infection by the pathogen and contribute to susceptibility. Loss of function of these S genes might provide broad-spectrum and durable disease resistance. Here, we screened S genes via comparative proteomic analysis between cucumber cultivars Rijiecheng and Superina, which exhibited resistance and high -susceptibility to FW, respectively. We identified 210 and 243 differentially regulated proteins (DRPs) in the Rijiecheng and Superina, respectively, and further found that 32 DRPs were predominantly expressed in Superina and significantly up-regulated after Foc inoculation. Expression verification found that TMEM115 (CsaV3_5G025750), encoding a transmembrane protein, TET8 (CsaV3_2G007840), encoding function as a tetraspanin, TPS10 (CsaV3_2G017980) encoding a terpene synthase, and MGT2 (CsaV3_7G006660), encoding a glycosyltransferase, were significantly induced in both cultivars after Foc infection but were induced to a higher expression level in Superina. These candidate genes might act as negative regulators of FW resistance in cucumber and provide effective FW-susceptibility gene resources for improving cucumber FW resistance through breeding programs.
Physical and Cognitive Functioning of Institutionalized Elderly People in Rural Areas. Preventive Actions Using Physical Activity and Music Therapy
Authors: González-Ojea María José , Domínguez-Lloria Sara , Portela-Pino Iago , Alvariñas-Villaverde Myriam
Published: November 2021

Background: Comprehensive geriatric evaluation should include a functional and cognitive assessment to guide the intervention of interdisciplinary teams. The aim of this study was to analyze the physical capacities of institutionalized elderly people and to describe the preventive actions of physical activity and music therapy as non-invasive preventive pharmacological treatments given their importance for the cognitive and functional performance of elderly people. An observational and descriptive cross-sectional study was carried out. The participants in the study were 109 elderly people institutionalized in three residential centers with a mean age of 83.41 years (SD = 8.72). Findings: Most of the residents had very impaired physical faculties. However, cognitive impairment was not very high. Most residents (55.04%) had some form of dementia and/or high blood pressure (54.12%) followed by pathologies such as diabetes (27.52%), heart failure (17.43%), Parkinson’s disease (9.17%) and chronic obstructive pulmonary disease (8.25%). There were no differences in cognitive or physical capacity among the residents according to sex, age, or education and only those who had worked in the service sector had less cognitive capacity than those who had worked in the agricultural sector or as housewives. Applications: Facilitate the creation and development of programs based on physical activity and music therapy in residential centers that can prevent and improve pathologies on the elderly.
Estimation of Diagnosis and Recommendation Integrated System (DRIS), Compositional Nutrient Diagnosis (CND) and Range of Normality (RN) Norms for Mineral Diagnosis of Almonds Trees in Spain
Authors: Ferrández-Cámara Mario , Martínez-Nicolás Juan José , Alfosea-Simón Marina , Cámara-Zapata José María , Melgarejo Moreno Pablo , García-Sánchez Francisco
Published: November 2021

To ensure good fertilization, it is necessary to know the optimum nutrient levels for each crop. The most common method for obtaining this information for almond trees is to perform a foliar analysis coupled with the use of interpretive tools such as the traditional range of normality. However, currently, there are other, more sophisticated methods such as the DRIS (Diagnosis and Recommendation Integrated System) and the CND (Compositional Nutrient Diagnosis) which take into account the relationship between nutrients. However, little information is available with respect to these methods in the case of almond trees. In the present work, 288 samples of three contrasting varieties of almond were analyzed—Ferraduel, Ferragnes, and Garrigues (Prunus dulcis, Mill.)—corresponding to bi-weekly sampling between the months of May and September. Leaf analysis data, run with different mathematical and statistical models, lead to knowledge of the optimum period for harvesting samples and the determination of the ranges of normality and norms of DRIS and CND for the Ferraduel, Ferragnes, and Garrigues varieties. Data gained from the leaf nutrient content reported that the best season to harvest and interpret leaf samples was July. In addition, Ferraduel and Ferragnes had higher N, P, and K (2.22, 0.14, and 1.04 mg Kg−1 dw, respectively) than Garrigues (2.00, 0.09. 0.67 mg Kg−1 dw). The norms obtained with the leaf mineral data showed similar values between the Ferraduel and Ferragnes varieties but different values for Garrigues variety. Therefore, Garriges had the highest N/P, N/K, P/K, and P × Mg norms in the DRIS method and the highest VN and VCa norms in the CND method.
Effects of C/N Ratio on Lignocellulose Degradation and Enzyme Activities in Aerobic Composting
Authors: Yang Huizhen , Zhang He , Qiu Huizhen , Anning Dominic Kwadwo , Li Mengchan , Wang Youling , Zhang Chunhong
Published: November 2021

Lignocellulosic materials have a complex physicochemical composition and structure that reduces their decomposition rate and hinders the formation of humic substances during composting. Therefore, a composting experiment was conducted to evaluate the effects of different C/N ratios on lignocellulose (cellulose, hemicellulose and lignin) degradation and the activities of corresponding enzymes during aerobic composting. The study had five C/N ratios, namely, T1 (C/N ratio of 15), T2 (C/N ratio of 20), T3 (C/N ratio of 25), T4 (C/N ratio of 30) and T5 (C/N ratio of 35). The results showed that treatments T3 and T4 had the highest rate of degradation of cellulose and hemicellulose, while treatment T3 had the highest rate of degradation of lignin. Among the five treatments, treatment T3 enhanced the degradation of the lignocellulose constituents, indicating a degradation rate of 6.86–35.17%, 15.63–44.08% and 31.69–165.60% for cellulose, hemicellulose and lignin, respectively. The degradation of cellulose and lignin occurred mainly at the thermophilic and late mesophilic phases of composting, while hemicellulose degradation occurred at the maturation phase. Treatment T3 was the best C/N ratio to stimulate the activities of manganese peroxidase, lignin peroxidase, polyphenol oxidase and peroxidase, which in turn promoted lignocellulose degradation.
Experimental Study on Chloride-Induced Corrosion of Soil Nail with Engineered Cementitious Composites (ECC) Grout
Authors: Wu Haoliang , Yu Jing , Zhou Jiajia , Li Weiwen , Leung Christopher K. Y.
Published: November 2021

Conventionally, a soil nail is a piece of steel reinforcement installed inside a hole drilled into the slope and filled with cement paste (CP) grout. Chloride penetration is a major deterioration mechanism of conventional soil nails as the CP grout is easy to crack with an uncontrollable crack opening when the soil nail is subject to loading or ground movements. Engineered Cementitious Composites (ECC) are a class of fiber-reinforced material exhibiting excellent crack control even when loaded to several percent of strain, and therefore, ECCs have great potential to replace traditional CP grout in soil nails for achieving a long service life. In this study, the chloride ion transport characteristics and electrically accelerated corrosion process of steel rebar in ECC and CP grouts are systematically studied. The rapid chloride ion penetration test results showed a reduction of 76% and 58% passing charges in ECC with 0.15% and 0.3% pre-loading strain, respectively, as compared to that in un-cracked CP. Furthermore, the accelerated corrosion experimental data showed that ECC under pre-loading strain still exhibited a coefficient of chloride ion diffusion that is 20–50% lower than CP grout due to the ability to control crack width. Service life calculations based on experimentally measured parameters showed that the predicted corrosion rate and corrosion depth of soil nails in ECC grout were much lower than those in CP grout. The findings can facilitate the design of soil nails with excellent durability and long service life.
Impacts of Phantom Off-Center Positioning on CT Numbers and Dose Index CTDIv: An Evaluation of Two CT Scanners from GE
Authors: Zheng Xiaoming , Gutsche Lachlan , Al-Hayek Yazan , Stanton Johanna , Elshami Wiam , Jensen Kelsey
Published: November 2021

The purpose of this work is to evaluate the impacts of body off-center positioning on CT numbers and dose index CTDIv of two scanners from GE. HD750 and APEX scanners were used to acquire a PBU60 phantom of Kagaku and a 062M phantom of CIRS respectively. CT images were acquired at various off-center positions under automatic tube current modulation using various peak voltages. CTDIv were recorded for each of the acquisitions. An abdomen section of the PBU60 phantom was used for CT number analysis and tissue inserts of the 062M phantom were filled with water balloons to mimic the human abdomen. CT numbers of central regions of interests were averaged using the Fiji software. As phantoms were lifted above the iso-center, both CTDIv and CT numbers were increased for the HD750 scanner whilst they were approximately constant for the APEX scanner. The measured sizes of anterior-posterior projection images were also increased for both scanners whilst the sizes of lateral projection images were increased for the HD750 scanner but decreased for the APEX scanner. Off-center correction algorithms were implemented in the APEX scanner. Matching the X-ray projection center with the system’s iso-center could improve the accuracy of CT imaging.
An Underwater Acoustic Target Recognition Method Based on Spectrograms with Different Resolutions
Authors: Luo Xinwei , Zhang Minghong , Liu Ting , Huang Ming , Xu Xiaogang
Published: November 2021

This paper focuses on the automatic target recognition (ATR) method based on ship-radiated noise and proposes an underwater acoustic target recognition (UATR) method based on ResNet. In the proposed method, a multi-window spectral analysis (MWSA) method is used to solve the difficulty that the traditional time–frequency (T–F) analysis method has in extracting multiple signal characteristics simultaneously. MWSA generates spectrograms with different T–F resolutions through multiple window processing to provide input for the classifier. Because of the insufficient number of ship-radiated noise samples, a conditional deep convolutional generative adversarial network (cDCGAN) model was designed for high-quality data augmentation. Experimental results on real ship-radiated noise show that the proposed UATR method has good classification performance.
Real-Time PCR Assay for the Detection of Dermatophytes: Comparison between an In-House Method and a Commercial Kit for the Diagnosis of Dermatophytoses in Patients from Dakar, Senegal
Authors: Kabtani Jihane , Diongue Khadim , Dione Jean-Noël , Delmas Anne , L’Ollivier Coralie , Amoureux Marie-Claude , Ndiaye Daouda , Ranque Stéphane
Published: November 2021

Background. PCR assays have been developed for the diagnosis of dermatophytes, yet data in African populations are scarce. Objective. This study aimed to compare two PCR assays for the diagnosis of dermatophytosis in outpatients at the Aristide Le Dantec University Hospital in Dakar, Senegal. Patients and methods. A total of 105 samples, including 24 skin, 19 nail and 62 hair samples collected from 99 patients were included in this study. Each sample was subjected to conventional diagnosis (CD), including direct microscopy and culture, and two real-time PCR assays: one in-house (IH)-PCR, used at the University Hospital of Marseille and the Eurobio Scientific commercial kit (CK): designed for the specific detection of six dermatophytes not including Microsporum audouinii. Results. Of the 105 specimens, 24.8%, 36.2% and 20% were positive by CD, IH-PCR and CK-PCR, respectively. The IH-PCR and CK-PCR exhibited 88.9% and 65.4% sensitivity, respectively. With a 36.6 diagnostic odd ratio and 1.41 needed to diagnose, the IH-PCR displayed better diagnostic indices than the CK-PCR. It is notable that, when considering the species that it claims to detect, when it came to skin and nail samples, CK-PCR sensitivity increased to 77%. Conclusions. The pan-dermatophyte IH-PCR performed better in the diagnosis of dermatophytosis in this African population than the CK-PCR, which is not designed to detect M. audouinii. Nevertheless, both assays exhibited similarly good diagnostic indices for tinea corporis and tinea unguium, both of which are localisations where M. audouinii is more rarely involved than in tinea capitis.
Application of BP Artificial Neural Network in Preparation of Ni–W Graded Coatings
Authors: Feng Pei , Shi Yuhua , Shang Peng , Wei Hanjun , Peng Tongtong , Pang Lisha , Feng Rongrong , Zhang Wenyuan
Published: November 2021

The internal stress difference between soft-ductile aluminum alloy substrate and hard-brittle Ni–W alloy coating will cause stress concentration, thus leading to the problem of poor bonding force. Herein, this work prepared the Ni–W graded coating on aluminum alloy matrix by the pulse electrodeposition method in order to solve the mechanical mismatch problem between substrate and coatings. More importantly, a backward propagation (BP) neural network was applied to efficiently optimize the pulse electrodeposition process of Ni–W graded coating. The SEM, EDS, XRD, Vickers hardness tester and Weighing scales are used to analyze the micromorphology, chemical element, phase composition, and micro hardness as well as oxidation weight increase, respectively. The results show that the optimal process conditions with BP neural network are as follows: the bath temperature is 30 °C, current density is 15 mA/cm2 and duty cycle is 0.3. The predicted value of the model agrees well with the experimental value curve, the relative error is minor. The maximum error is less than 3%, and the correlation coefficient is 0.9996. The Ni–W graded coating prepared by BP neural network shows good bonding with the substrate which has flat and smooth interface. The thickness of the coating is about 136 μm, which slows down the oxidation of the substrate and plays an effective role in protecting the substrate.
A Particle Swarm Optimisation with Linearly Decreasing Weight for Real-Time Traffic Signal Control
Authors: Shi Yanjun , Qi Yuhan , Lv Lingling , Liang Donglin
Published: November 2021

Nowadays, traffic congestion has become a significant challenge in urban areas and densely populated cities. Real-time traffic signal control is an effective method to reduce traffic jams. This paper proposes a particle swarm optimisation with linearly decreasing weight (LDW-PSO) to tackle the signal intersection control problem, where a finite-interval model and an objective function are built to minimise spoilage time. The performance was evaluated in real-time simulation imitating a crowded intersection in Dalian city (in China) via the SUMO traffic simulator. The simulation results showed that the LDW-PSO outperformed the classical algorithms in this research, where queue length can be reduced by up to 20.4% and average waiting time can be reduced by up to 17.9%.
ARDL as an Elixir Approach to Cure for Spurious Regression in Nonstationary Time Series
Authors: Ghouse Ghulam , Khan Saud Ahmad , Rehman Atiq Ur , Bhatti Muhammad Ishaq
Published: November 2021

In conventional Econometrics, the unit root and cointegration analysis are the only ways to circumvent the spurious regression which may arise from missing variable (lag values) rather than the nonstationarity process in time series data. We propose the Ghouse equation solution of autoregressive distributed lag mechanism which does not require additional work in unit root testing and bound testing. This advantage makes the proposed methodology more efficient compared to the existing cointegration procedures. The earlier tests weaken their position in comparison to it, as they had numerous linked testing procedures which further increase the size of the test and/or reduce the test power. The simplification of the Ghouse equation does not attain any such type of error, which makes it a more powerful test as compared to widely cited exiting testing methods in econometrics and statistics literature.
Trabecular Bone Score and Bone Mineral Density in Postmenopausal Women with Morbid Obesity—A Clinical Paradox
Authors: Jose Antresa , Cherian Kripa Elizabeth , Nandyal Munaf Babajan , Jiwanmall Stephen A. , Kattula Dheeraj , Paul Thomas V. , Kapoor Nitin
Published: November 2021

Obesity has long been considered to have a protective effect on bone, but specific complications in those with morbid obesity are known to have a detrimental impact on bone architecture. We aimed to study the bone microarchitecture (TBS—trabecular bone score) and bone mineral density (BMD) in postmenopausal women with morbid obesity compared to obese and non-obese age-matched women. Eighty-five consecutive postmenopausal women with morbid obesity (body mass index (BMI) ≥ 35 kg/m2) were enrolled and compared to age-matched obese (n = 80) and non-obese postmenopausal controls (n = 85). The BMD and TBS were assessed in all subjects using a Hologic-QDR 4500-W Discovery-A DXA scanner. The mean BMD (gm/cm2) at the femoral neck in women with morbid obesity was found to be significantly lower as compared to the age-matched postmenopausal obese controls (0.723 versus 0.762, p-value = 0.002). The BMD at the lumbar spine and hip showed similar trends but were not statistically significant. The bone microarchitecture was found to be significantly lower in those with morbid obesity (1.205) as compared to the other two groups (obesity 1.244; non-obese 1.228) (p < 0.013). Though obesity was associated with a better bone density and bone microarchitecture in postmenopausal women, a paradoxical lower value was seen in those with morbid obesity.
Organic Solvent Nanofiltration of Water-in-Oil Pickering Emulsions—What Influences Permeability?
Authors: Kempin Maresa Vivien , Drews Anja
Published: November 2021

Pickering emulsions (PEs) have received increasing interest for their application in catalytic multiphase reactions. Organic solvent nanofiltration of PEs was shown to be a promising procedure for efficient and effective catalyst recycling. In this work, a systematic parameter study to identify the main influencing parameters on PE filtration was conducted for a large variety of PE compositions for the first time. In addition to temperature, only the type of organic solvent significantly influenced the filtration performance, which could be mathematically modeled via a combination of the solution–diffusion and the resistance in the series model. Particle type and concentration, dispersed phase fraction and the presence of reaction (by-)products did not show any significant impact on the permeability. The stirrer speed only became important when emulsions stabilized by particles without the tendency to form 3D network structures were filtered in long-term filtration experiments. These results pave the way towards the application of PE membrane filtration for catalyst recovery in continuous liquid/liquid multiphase reactions and enable broad operation windows. As the mechanical separation of PEs was shown to be a very robust process, the emulsion composition can now be tuned to meet the needs of the reaction without any (significant) loss in filtration performance.
Attachment of Shiga Toxin-Producing Escherichia coli (STEC) to Pre-Chill and Post-Chill Beef Brisket Tissue
Authors: Unruh Daniel A. , Uhl Bennett C. , Phebus Randall K. , Gragg Sara E.
Published: November 2021

Shiga toxin-producing Escherichia coli (STEC) has caused numerous foodborne illness outbreaks where beef was implicated as the contaminated food source. Understanding how STEC attach to beef surfaces may inform effective intervention applications at the abattoir. This simulated meat processing conditions to measure STEC attachment to adipose and lean beef tissue. Beef brisket samples were warmed to a surface temperature of 30 °C (warm carcass), while the remaining samples were maintained at 4 °C (cold carcass), prior to surface inoculation with an STEC cocktail (O26, O45, O103, O111, O121, O145, and O157:H7). Cocktails were grown in either tryptic soy broth (TSB) or M9 minimal nutrient medium. Loosely and firmly attached cells were measured at 0, 3, 5, and 20 min and 1, 3, 8, 12, 24 and 48 h. TSB-grown STEC cells became more firmly attached throughout storage and a difference in loosely versus firmly attached populations on lean and adipose tissues was observed. M9-grown STEC demonstrated a 0.2 log10 CFU/cm2 difference in attachment to lean versus adipose tissue and variability in populations was recorded throughout sampling. Future research should investigate whether a decrease in intervention efficacy correlates to an increase in firmly attached STEC cells on chilled carcasses and/or subprimals, which has been reported.