bluuebunny/crossref_metadata_2025 huggingface dataset:
Mobilising Collaboration among Stakeholders to Optimise the Growing Potential of Data for Tackling Cancer
Authors: Horgan Denis , Van den Bulcke Marc , Malapelle Umberto , Normanno Nicola , Capoluongo Ettore D. , Prelaj Arsela , Rizzari Carmelo , Stathopoulou Aliki , Singh Jaya , Kozaric Marta , Dube France , Ottaviano Manuel , Boccia Stefania , Pravettoni Gabriella , Cattaneo Ivana , Malats Núria , Buettner Reinhard , Lekadir Karim , de Lorenzo Francesco , Alix-Panabieres Catherine , Badreh Sara , Hofman Paul , De Maria Ruggero , Solary Eric
Published: October 2023

Effective cancer diagnosis, treatment and control depend on interactions among numerous distinct factors, from technology to data to skills to sociology. But a crucial influence is the extent to which the health system takes account of the distinct perspectives of the many different groups of interdependent stakeholders concerned with cancer, including patients, practitioners and planners. This paper provides some elucidation as to how far and how efficiently these interactions currently take place in Europe. It also makes some tentative suggestions as to how conscious systematic interventions could improve cancer outcomes. It is based on a series of expert panels and surveys conducted by the European Alliance for Personalised Medicine (EAPM) that provided information at the national level on three selected parameters: implementation of next-generation sequencing (NGS) and liquid biopsy (LB), attitudes of patients to prevention and practices of sharing genomic data among healthcare professionals (HCPs). The varying data infrastructure highlights the urgent need for substantial improvements to accommodate the increasing importance of genomics data in cancer diagnosis and care. Additionally, we identify disparities in age-specific approaches to cancer prevention, emphasising the necessity for tailored strategies to address unique age group perspectives. Moreover, distinct regional prioritizations in cancer treatment underscore the importance of considering regional variations when shaping future cancer care strategies. This study advocates for collaborative data sharing supported by technological innovation to overcome these challenges, ultimately fostering a holistic and equitable provision of cancer care in Europe.
Traversing the Cell Wall: The Chitinolytic Activity of Histoplasma capsulatum Extracellular Vesicles Facilitates Their Release
Authors: Valdez Alessandro F. , de Souza Taiane Nascimento , Bonilla Jhon Jhamilton Artunduaga , Zamith-Miranda Daniel , Piffer Alicia Corbellini , Araujo Glauber R. S. , Guimarães Allan J. , Frases Susana , Pereira Alana Kelyene , Fill Taicia Pacheco , Estevao Igor L. , Torres Angel , Almeida Igor C. , Nosanchuk Joshua D. , Nimrichter Leonardo
Published: October 2023

Histoplasma capsulatum is the causative agent of histoplasmosis. Treating this fungal infection conventionally has significant limitations, prompting the search for alternative therapies. In this context, fungal extracellular vesicles (EVs) hold relevant potential as both therapeutic agents and targets for the treatment of fungal infections. To explore this further, we conducted a study using pharmacological inhibitors of chitinase (methylxanthines) to investigate their potential to reduce EV release and its subsequent impact on fungal virulence in an in vivo invertebrate model. Our findings revealed that a subinhibitory concentration of the methylxanthine, caffeine, effectively reduces EV release, leading to a modulation of H. capsulatum virulence. To the best of our knowledge, this is the first reported instance of a pharmacological inhibitor that reduces fungal EV release without any observed fungicidal effects.
A New Exopolysaccharide of Marine Coral-Associated Aspergillus pseudoglaucus SCAU265: Structural Characterization and Immunomodulatory Activity
Authors: Peng Bo , Liu Yongchun , Lin Yuqi , Kraithong Supaluck , Mo Li , Gao Ziqing , Huang Riming , Zhang Xiaoyong
Published: October 2023

Recent studies have found that many marine microbial polysaccharides exhibit distinct immune activity. However, there is a relative scarcity of research on the immunomodulatory activity of marine fungal exopolysaccharides. A novel water-soluble fungal exopolysaccharide ASP-1 was isolated from the fermentation broths of marine coral-associated fungus Aspergillus pseudoglaucus SCAU265, and purified by Diethylaminoethyl-Sepharose-52 (DEAE-52) Fast Flow and Sephadex G-75. Structural analysis revealed that ASP-1 had an average molecular weight of 36.07 kDa and was mainly composed of (1→4)-linked α-D-glucopyranosyl residues, along with highly branched heteropolysaccharide regions containing 1,4,6-glucopyranosyl, 1,3,4-glucopyranosyl, 1,4,6-galactopyranosyl, T(terminal)-glucopyranosyl, T-mannopyranosyl, and T-galactopyranosyl residues. ASP-1 demonstrated significant effects on the proliferation, nitric oxide levels, and the secretion of cytokines TNF-α and IL-6 in macrophage RAW264.7 cells. Metabolomic analysis provided insights into the potential mechanisms of the immune regulation of ASP-1, suggesting its involvement in regulating immune function by modulating amino acid anabolism, particularly arginine synthesis and metabolism. These findings provide fundamental scientific data for further research on its accurate molecular mechanism of immunomodulatory activity.
Regional versus General Anesthesia in Postoperative Pain Management after Distal Radius Fracture Surgery: Meta-Analysis of Randomized Controlled Trials
Authors: Roh Young Hak , Park Seong Gyun , Lee Seung Hyun
Published: October 2023

Distal radius fractures are the most prevalent upper extremity fractures, posing a significant public health concern. Recent studies comparing regional and general anesthesia for postoperative pain management after these fractures have yielded conflicting results. This meta-analysis aimed to compare the effectiveness of regional and general anesthesia concerning postoperative pain management and opioid consumption following distal radius fracture surgery. A comprehensive search was conducted in PubMed, Cochrane Library, and EMBASE databases to identify relevant randomized controlled trials. Four randomized trials involving 248 participants were included in the analysis. A pooled analysis revealed that regional anesthesia led to significantly reduced postoperative pain scores at 2 h compared to general anesthesia (SMD −2.03; 95% CI −2.88–−1.17). However, no significant differences in pain scores were observed between the two anesthesia types after 12 h post-surgery. Regional anesthesia was associated with lower total opioid consumption (SMD −0.76; 95% CI −1.25–−0.26) and fewer occurrences of nausea and vomiting compared to the general anesthesia. Nonetheless, opioid consumption on the first day post-discharge was significantly higher in the regional anesthesia group (SMD 0.83; 95% CI 0.47–1.20). The analgesic superiority of regional anesthesia is confined to the early postoperative hours with overall lower opioid use but a notable increase in opioid consumption on the first day post-discharge, potentially attributable to rebound pain.
Comparative Assessment of the Spatiotemporal Dynamics and Driving Forces of Natural and Constructed Wetlands in Arid and Semiarid Areas of Northern China
Authors: Zhang Jian , Qin Yao , Zhang Yuxuan , Lu Xin , Cao Jianjun
Published: October 2023

Arid and semiarid wetlands, the core geographical unit of desert oases, significantly benefit and improve the ecological environment. In this study, we systematically compared the spatiotemporal dynamics and driving forces of natural and constructed wetlands in arid and semiarid regions of northern China from 1995 to 2019. For these comparisons, we utilized a land-use transition matrix, partial least-squares–structural equation model (PLS-SEM), and geographically weighted regression (GWR) model. The results showed that (1) the area of wetlands as a whole showed an upward trend, with natural and constructed wetlands increasing by 4.16% and 11.86%, respectively. The increases mainly resulted from conversions of grassland and other lands (shrub, sparse vegetation, and bare land). (2) The direct dominant factors that drove natural wetland changes were soil and terrain, while those that drove constructed wetlands were human disturbances. Human disturbance, by affecting soil, had a higher significant indirect effect on natural wetlands. Heat, by affecting moisture, had the greatest indirect effect on constructed wetlands. (3) The sensitivity of natural and constructed wetlands to the responses of different drivers showed significant spatial heterogeneity. This study explores the interaction and driving mechanisms of human and natural attributes on natural and constructed wetlands and provides a scientific foundation for the restoration and sustainable development of wetlands in arid and semiarid areas.
Automatic Skin Cancer Detection Using Clinical Images: A Comprehensive Review
Authors: Nazari Sana , Garcia Rafael
Published: October 2023

Skin cancer has become increasingly common over the past decade, with melanoma being the most aggressive type. Hence, early detection of skin cancer and melanoma is essential in dermatology. Computational methods can be a valuable tool for assisting dermatologists in identifying skin cancer. Most research in machine learning for skin cancer detection has focused on dermoscopy images due to the existence of larger image datasets. However, general practitioners typically do not have access to a dermoscope and must rely on naked-eye examinations or standard clinical images. By using standard, off-the-shelf cameras to detect high-risk moles, machine learning has also proven to be an effective tool. The objective of this paper is to provide a comprehensive review of image-processing techniques for skin cancer detection using clinical images. In this study, we evaluate 51 state-of-the-art articles that have used machine learning methods to detect skin cancer over the past decade, focusing on clinical datasets. Even though several studies have been conducted in this field, there are still few publicly available clinical datasets with sufficient data that can be used as a benchmark, especially when compared to the existing dermoscopy databases. In addition, we observed that the available artifact removal approaches are not quite adequate in some cases and may also have a negative impact on the models. Moreover, the majority of the reviewed articles are working with single-lesion images and do not consider typical mole patterns and temporal changes in the lesions of each patient.
The Evolution of Circulating Biomarkers for Use in Acetaminophen/Paracetamol-Induced Liver Injury in Humans: A Scoping Review
Authors: McGill Mitchell R. , Curry Steven C.
Published: October 2023

Acetaminophen (APAP) is a widely used drug, but overdose can cause severe acute liver injury. The first reports of APAP hepatotoxicity in humans were published in 1966, shortly after the development of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) as the first biomarkers of liver injury as opposed to liver function. Thus, the field of liver injury biomarkers has evolved alongside the growth in APAP hepatotoxicity incidence. Numerous biomarkers have been proposed for use in the management of APAP overdose patients in the intervening years. Here, we comprehensively review the development of these markers from the 1960s to the present day and briefly discuss possible future directions.
Effect of Film Thickness on the Self-Assembly of CBABC Symmetric Pentablock Terpolymer Melts under 1D Confinement: A Dissipative Particle Dynamic Study
Authors: Guo Yingying
Published: October 2023

The study investigates the impact of film thickness on the phase behavior of pentablock terpolymers, denoted as C3B3A6B3C3, when subjected to wall confinement by utilizing the dissipative particle dynamics method. Phase diagrams were constructed to elucidate how factors such as block–block interaction strength, film thickness, and wall properties affect the self-assembly structures. In cases where the wall exhibits no preference for any of the blocks, lamellae phases with orientations perpendicular to the wall are observed. The order–disorder transition (ODT) temperature is found to be influenced by the interaction between the polymer and the wall in thin confinement scenarios. When the wall displays a preference for specific blocks, the orientation of lamellae structures undergoes variations. Lamellae tend to align parallel to the wall when the wall favors A or C blocks, and they orient perpendicularly when B blocks are favored. Furthermore, the mechanical properties of the lamellae structures are related to the conformations of the polymer chains. Structures where chains predominantly adopt a loop conformation exhibit enhanced elastic properties. The ratio of looping to bridging conformations can be adjusted by altering the film thickness and wall selectivity.
Evolution of CrCx Ceramic Induced by Laser Direct Energy Deposition Multilayered Gradient Ni204-dr60 Coating
Authors: Zhao Yu , Wang Ruobing , Zhang Jian , Farid Muhammad Imran , Wu Wenzheng , Yu Tianbiao
Published: October 2023

The manufacturing process for many large components of machines leads to a difference in their properties and performances based on changes in location. Functionally graded materials can meet these requirements and address the issue of generation and expansion of interface cracks. Ni204–dr60 gradient coatings were successfully fabricated using laser direct energy deposition (LDED). Microstructure mechanism evolution and microhardness of the gradient coating were comprehensively investigated. The change in the precipitated phase at the grain boundary and the intergranular zones resulted in a change in microstructural characteristics and also affected the microhardness distribution. The reinforced phase of the Ni204 → dr60 gradient zone from Ni204 to dr60 gradually precipitated and was rich in Mo and Nb phase, lath-shaped CrCx phase, network-shaped CrCx phase, block shape (Ni, Si) (C, B) phase, block CrCx phase, and block Cr (B, C) phase. The gradient coating thus acts as a potential candidate to effectively solve the problem of crack generation at the interface of dr60 and the substrate.
Design of Variable Stiffness Trajectories with Cubic Ferguson Curve
Authors: Zhang Deli , Wang Kai , Wang Xiaoping
Published: October 2023

To design a class of full cover trajectories that satisfy curvature limitations and enhance the buckling load of constructed laminates, a variable stiffness laminate is proposed by applying the cubic Ferguson curve. First, the traditional explicit form of the cubic Ferguson curve is redefined as polar coordinates, two connected Ferguson curve segments with three extra parameters are applied to describe full cover trajectories, and the effects on trajectories introduced by these modifications are discussed. Then, the finite element method is used to introduce parameters for analyzing the buckling load of the designed variable stiffness laminates. Numerical experiments show that automatic fiber placement (AFP) trajectories described by the cubic Ferguson curve can automatically reach C1 continuity and can be locally modified by adjusting the introduced parameters. Compared with traditional constant stiffness laminates, the variable stiffness laminates designed using the proposed method exhibit a higher buckling load and better stability.
The Impact of Al2O3 Particles from Grit-Blasted Ti6Al7Nb (Alloy) Implant Surfaces on Biocompatibility, Aseptic Loosening, and Infection
Authors: Kocjančič Boštjan , Avsec Klemen , Šetina Batič Barbara , Feizpour Darja , Godec Matjaž , Kralj-Iglič Veronika , Podlipec Rok , Cör Andrej , Debeljak Mojca , Grant John T. , Jenko Monika , Dolinar Drago
Published: October 2023

For the improvement of surface roughness, titanium joint arthroplasty (TJA) components are grit-blasted with Al2O3 (corundum) particles during manufacturing. There is an acute concern, particularly with uncemented implants, about polymeric, metallic, and corundum debris generation and accumulation in TJA, and its association with osteolysis and implant loosening. The surface morphology, chemistry, phase analysis, and surface chemistry of retrieved and new Al2O3 grit-blasted titanium alloy were determined with scanning electron microscopy (SEM), X-ray energy-dispersive spectroscopy (EDS), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and confocal laser fluorescence microscopy, respectively. Peri-prosthetic soft tissue was studied with histopathology. Blasted retrieved and new stems were exposed to human mesenchymal stromal stem cells (BMSCs) for 7 days to test biocompatibility and cytotoxicity. We found metallic particles in the peri-prosthetic soft tissue. Ti6Al7Nb with the residual Al2O3 particles exhibited a low cytotoxic effect while polished titanium and ceramic disks exhibited no cytotoxic effect. None of the tested materials caused cell death or even a zone of inhibition. Our results indicate a possible biological effect of the blasting debris; however, we found no significant toxicity with these materials. Further studies on the optimal size and properties of the blasting particles are indicated for minimizing their adverse biological effects.
Recent Advances in Sodium-Ion Batteries: Cathode Materials
Authors: Nguyen Thang Phan , Kim Il Tae
Published: October 2023

Emerging energy storage systems have received significant attention along with the development of renewable energy, thereby creating a green energy platform for humans. Lithium-ion batteries (LIBs) are commonly used, such as in smartphones, tablets, earphones, and electric vehicles. However, lithium has certain limitations including safety, cost-effectiveness, and environmental issues. Sodium is believed to be an ideal replacement for lithium owing to its infinite abundance, safety, low cost, environmental friendliness, and energy storage behavior similar to that of lithium. Inhered in the achievement in the development of LIBs, sodium-ion batteries (SIBs) have rapidly evolved to be commercialized. Among the cathode, anode, and electrolyte, the cathode remains a significant challenge for achieving a stable, high-rate, and high-capacity device. In this review, recent advances in the development and optimization of cathode materials, including inorganic, organometallic, and organic materials, are discussed for SIBs. In addition, the challenges and strategies for enhancing the stability and performance of SIBs are highlighted.
Prolonging the Durability of Maritime Constructions through a Sustainable and Salt-Resistant Cement Composite
Authors: Heikal Mohamed , Ali Mohamed A. , Ghernaout Djamel , Elboughdiri Noureddine , Ghernaout Badia , Bendary Hazem I.
Published: October 2023

This research investigates the long-term resilience of an environmentally friendly cement blend comprising Egyptian Ordinary Portland Cement OPC and Ground-Granulated Blast Furnace Slag GGBFS when exposed to a corrosive seawater environment. This scientific investigation explores the effects of exposure to seawater on various properties of cement pastes, encompassing parameters such as free lime content (FLC), chemically combined water content (CWC), bulk density (BD), total porosity (ϕ), total sulfate content, total chloride content, and compressive strength (CS). By contrast, Differential Thermal Analysis (DTA), FT-IR spectroscopy, and X-ray diffraction (XRD) analysis can be utilized to investigate the influence of exposure to seawater on the hydration products of GGBFS cement pastes over a period of up to one year. This analytical approach offers valuable insights into the alterations that occur in hydration products and their resilience when subjected to seawater conditions. The results obtained from this investigation reveal that all cement pastes incorporating GGBFS exhibit heightened resistance to deterioration in seawater, with slag cement containing 60 wt. % GGBFS and achieving a notable compressive strength of 85.7 Mpa after one year of immersion in seawater. These findings underscore the capacity of these cement blends to effectively withstand challenges in durability in marine environments.
Synthesis and Characterization of Bioactive Glass and Zinc Oxide Nanoparticles with Enamel Remineralization and Antimicrobial Capabilities
Authors: Nagasaki Ryota , Nagano Keiji , Nezu Takashi , Iijima Masahiro
Published: October 2023

Background: We investigated the effect of bioactive glass and zinc oxide nanoparticles on enamel remineralization, as well as their antimicrobial effect on cariogenic microbes. This is the first study that investigated the properties of bioactive glass and zinc oxide nanoparticles with mixed materials. Methods: Fluoride gel (F), bioactive glass microparticles (µB), bioactive glass nanoparticles (nB), zinc oxide nanoparticles (Z), and a mixed suspension of nB and Z (nBZ) were prepared and characterized by scanning and transmission electron microscopy, zeta potential measurement, X-ray diffraction, and acid buffering capacity testing. Further, we performed a remineralization cycle test of 28 days, and nanoindentation testing was carried out during the immersion period, and then the enamel surfaces were examined using scanning electron microscopy. Additionally, the antimicrobial effects of the sample suspensions were evaluated by measuring their minimum microbicidal concentrations against various cariogenic microbes. Results: Our results revealed that nB had a near-circular shape with an amorphous structure and a considerably large specific surface area due to nanoparticulation. Additionally, nB possessed a rapid acid buffering capacity that was comparable to that of μB. In the remineralization test, faster recovery of mechanical properties was observed on the enamel surface immersed in samples containing bioactive glass nanoparticles (nB and nBZ). After remineralization, demineralized enamel immersed in any of the samples showed a rough and porous surface structure covered with mineralized structures. Furthermore, nBZ exhibited a broad antimicrobial spectrum. Conclusions: These results demonstrated that bioactive glass and zinc oxide nanoparticles have superior demineralization-suppressing and remineralization-promoting effects.
On Indices of Septic Number Fields Defined by Trinomials x7 + ax + b
Authors: El Fadil Lhoussain
Published: October 2023

Let K be a septic number field generated by a root, α, of an irreducible trinomial, x7+ax+b∈Z[x]. In this paper, for every prime integer, p, we calculate νp(i(K)); the highest power of p dividing the index, i(K), of the number field, K. In particular, we calculate the index, i(K). In application, when the index of K is not trivial, then K is not monogenic.
Periodic Flows in a Viscous Stratified Fluid in a Homogeneous Gravitational Field
Authors: Chashechkin Yuli D. , Ochirov Artem A.
Published: October 2023

The density of a fluid or gas, which depends on the temperature, pressure and concentration of dissolved substances or suspended particles, changes under the influence of a large number of physical factors. We assume that an undisturbed liquid is heterogeneous. The propagation of periodic flows in viscous, uniformly stratified fluids is considered. The analysis is based on a system of fundamental equations for the transfer of energy, momentum and matter in periodic flows. Taking into account the compatibility condition, dispersion relations are constructed for two-dimensional internal, acoustic and surface linear periodic flows with a positive definite frequency and complex wave number in a compressible viscous fluid exponentially stratified by density. The temperature conductivity and diffusion effects are neglected. The obtained regularly perturbed solutions of the dispersion equations describe the conventional weakly damped waves. The families of singular solutions, specific for every kind of periodic flow, characterize the before unknown thin ligaments that accompany each type of wave. In limited cases, the constructed regular solutions transform into well-known expressions for a viscous homogeneous and an ideal fluid. Singular solutions are degenerated in a viscous homogeneous fluid or disappear in an ideal fluid. The developing method of the fundamental equation system analysis is directed to describe the dynamics and spatial structure of periodic flows in heterogeneous fluids in linear and non-linear approximations.
An Improved WENO-Z Scheme for Hyperbolic Conservation Laws with New Global Smoothness Indicator
Authors: Han Shuang , Li Mingjun
Published: October 2023

The fifth-order WENO-Z scheme proposed by Borges et al., using a linear combination of low-order smoothness indicators, is designed to provide a low numerical dissipation to solve hyperbolic conservation laws, while the power q in the framework of WENO-Z plays a key role in its performance. In this paper, a novel global smoothness indicator with fifth-order accuracy, which is based on several lower-order smoothness indicators on two-point sub-stencils, is presented, and a new lower-dissipation WENO-Z scheme (WENO-NZ) is developed. The spectral properties of the WENO-NZ scheme are studied through the ADR method and show that this new scheme can exhibit better spectral results than WENO-Z no matter what the power value is. Accuracy tests confirm that the accuracy of WENO-Z with q = 1 would degrade to the fourth order at first-order critical points, while WENO-NZ can recover the optimal fifth-order convergence. Furthermore, numerical experiments with one- and two-dimensional benchmark problems demonstrate that the proposed WENO-NZ scheme can efficiently decrease the numerical dissipation and has a higher resolution compared to the WENO-Z scheme.
Similarity Classes in the Eight-Tetrahedron Longest-Edge Partition of a Regular Tetrahedron
Authors: Padrón Miguel A. , Plaza Ángel , Suárez José Pablo
Published: October 2023

A tetrahedron is called regular if its six edges are of equal length. It is clear that, for an initial regular tetrahedron R0, the iterative eight-tetrahedron longest-edge partition (8T-LE) of R0 produces an infinity sequence of tetrahedral meshes, τ0={R0}, τ1={Ri1}, τ2={Ri2},…, τn={Rin},…. In this paper, it is proven that, in the iterative process just mentioned, only two distinct similarity classes are generated. Therefore, the stability and the non-degeneracy of the generated meshes, as well as the minimum and maximum angle condition straightforwardly follow. Additionally, for a standard-shape tetrahedron quality measure (η) and any tetrahedron Rin∈τn, n>0, then ηRin≥23ηR0. The non-degeneracy constant is c=23 in the case of the iterative 8T-LE partition of a regular tetrahedron.
Research on Emotional Infection of Passengers during the SRtP of a Cruise Ship by Combining an SIR Model and Machine Learning
Authors: Xiong Gaohan , Cai Wei , Hu Min , Yu Zhiyan
Published: October 2023

The Safe Return to Port issue regarding cruise ships has been extensively researched, covering aspects such as performance, operations, and electrical systems. However, an often overlooked aspect is the potential eruption of negative emotions among passengers during SRtP. This study aims to investigate the prediction of collective emotions to facilitate timely safety planning and enhance the safety of the Safe Return to Port process. To achieve this objective, an improved susceptible-infectious-recovered model with bidirectional infection is proposed to describe the emotional contagion process during the Safe Return to Port process. This model classifies the population into five emotional (extremely anxious–anxious–normal–calm–very calm) states and introduces two sources of infection. Moreover, it allows for emotions to transition both positively and negatively, making it a more realistic representation of scenarios resembling long-term refuge scenarios. In this study, questionnaire data, collected and statistically analyzed, serve as the primary dataset. A machine learning technique (the weighted random forest algorithm) is integrated with the model to make predictions. The accuracy, precision, recall, and the F-measure of prediction results demonstrate good performance. Additionally, through simulation, this study illustrates the fluctuating nature of emotional changes during the Safe Return to Port process of the cruise ship and analyzes the effects of varying parameters. The findings suggest that the improved susceptible-infectious-recovered model proposed in this paper can provide valuable insights for cruise ship emergency planning and positively contribute to maintaining passenger emotional stability during the Safe Return to Port process.
Structural Characterization and Cytotoxic Activity Evaluation of Ulvan Polysaccharides Extracted from the Green Algae Ulva papenfussii
Authors: Tran Vy Ha Nguyen , Mikkelsen Maria Dalgaard , Truong Hai Bang , Vo Hieu Nhu Mai , Pham Thinh Duc , Cao Hang Thi Thuy , Nguyen Thuan Thi , Meyer Anne S. , Thanh Thuy Thu Thi , Van Tran Thi Thanh
Published: October 2023

Ulvan, a sulfated heteropolysaccharide with structural and functional properties of interest for various uses, was extracted from the green seaweed Ulva papenfussii. U. papenfussii is an unexplored Ulva species found in the South China Sea along the central coast of Vietnam. Based on dry weight, the ulvan yield was ~15% (w/w) and the ulvan had a sulfate content of 13.4 wt%. The compositional constitution encompassed L-Rhamnose (Rhap), D-Xylose (Xylp), D-Glucuronic acid (GlcAp), L-Iduronic acid (IdoAp), D-Galactose (Galp), and D-Glucose (Glcp) with a molar ratio of 1:0.19:0.35:0.52:0.05:0.11, respectively. The structure of ulvan was determined using High-Performance Liquid Chromatography (HPLC), Fourier Transform Infrared Spectroscopy (FT-IR), and Nuclear Magnetic Resonance spectroscopy (NMR) methods. The results showed that the extracted ulvan comprised a mixture of two different structural forms, namely (“A3s”) with the repeating disaccharide [→4)-β-D-GlcAp-(1→4)-α-L-Rhap 3S-(1→]n, and (“B3s”) with the repeating disaccharide [→4)-α-L-IdoAp-(1→4)-α-L-Rhap 3S(1→]n. The relative abundance of A3s, and B3s was 1:1.5, respectively. The potential anticarcinogenic attributes of ulvan were evaluated against a trilogy of human cancer cell lineages. Concomitantly, Quantitative Structure–Activity Relationship (QSAR) modeling was also conducted to predict potential adverse reactions stemming from pharmacological interactions. The ulvan showed significant antitumor growth activity against hepatocellular carcinoma (IC50 ≈ 90 µg/mL), human breast cancer cells (IC50 ≈ 85 µg/mL), and cervical cancer cells (IC50 ≈ 67 µg/mL). The QSAR models demonstrated acceptable predictive power, and seven toxicity indications confirmed the safety of ulvan, warranting its candidacy for further in vivo testing and applications as a biologically active pharmaceutical source for human disease treatment.
In Vitro Anti-Inflammatory Activity and Structural Characteristics of Polysaccharides Extracted from Lobonema smithii Jellyfish
Authors: Summat Thitikan , Wangtueai Sutee , You SangGuan , Rod-in Weerawan , Park Woo Jung , Karnjanapratum Supatra , Seesuriyachan Phisit , Surayot Utoomporn
Published: October 2023

Crude polysaccharides were extracted from the white jellyfish (Lobonema smithii) using water extraction and fractionated using ion-exchange chromatography to obtain three different fractions (JF1, JF2, and JF3). The chemical characteristics of four polysaccharides were investigated, along with their anti-inflammatory effect in LPS-stimulated RAW264.7 cells. All samples mainly consisted of neutral sugars with minor contents of proteins and sulphates in various proportions. Glucose, galactose, and mannose were the main constituents of the monosaccharides. The molecular weights of the crude polysaccharides and the JF1, JF2, and JF3 fractions were 865.0, 477.6, 524.1, and 293.0 kDa, respectively. All polysaccharides were able to decrease NO production, especially JF3, which showed inhibitory activity. JF3 effectively suppressed iNOS, COX-2, IL-1β, IL-6, and TNF-α expression, while IL-10 expression was induced. JF3 could inhibit phosphorylated ERK, JNK, p38, and NF-κB p65. Furthermore, flow cytometry showed the impact of JF3 on inhibiting CD11b and CD40 expression. These results suggest that JF3 could inhibit NF-κB and MAPK-related inflammatory pathways. The structural characterisation revealed that (1→3)-linked glucopyranosyl, (1→3,6)-linked galactopyranosyl, and (1→3,6)-linked glucopyranosyl residues comprised the main backbone of JF3. Therefore, L. smithii polysaccharides exhibit good anti-inflammatory activity and could thus be applied as an alternative therapeutic agent against inflammation.
Understanding Solid-Based Platelet-Rich Fibrin Matrices in Oral and Maxillofacial Surgery: An Integrative Review of the Critical Protocol Factors and Their Influence on the Final Product
Authors: Salgado-Peralvo Ángel-Orión , Kewalramani Naresh , Pérez-Jardón Alba , Pato-Mourelo Jesús , Castro-Calderón Adriana , Arriba-Fuente Lorenzo , Pérez-Sayáns Mario
Published: October 2023

Platelet-rich fibrin (PRF) is a second-generation platelet concentrate whose use in clinical practice has been widely disseminated. This has led to the development of several commercial protocols, creating great confusion as to the terminology and implications of each of them. This integrative review aims to identify the critical factors of each of the phases of the solid-based PRF matrix protocol and their possible influence on their macro- and microscopic characteristics. An electronic search of the MEDLINE database (via PubMed), Web of Science, Scopus, LILACS, and OpenGrey was carried out. The search was temporarily restricted from 2001 to 2022. After searching, 43 studies were included that met the established criteria. There were numerous factors to consider in the PRF protocol, such as the material of the blood collection tubes, the duration of phlebotomy, the parameters related to blood centrifugation, the time from centrifugation to dehydration of the fibrin clots and their dehydration into membranes, as well as the time to clinical use. These factors influenced the macro- and microscopic characteristics of the PRF and its physical properties, so knowledge of these factors allows for the production of optimised PRF by combining the protocols and materials.
Kynurenine, Kynurenic Acid, Quinolinic Acid and Interleukin-6 Levels in the Serum of Patients with Autism Spectrum Disorder
Authors: Yildirim Veli , Simsek Seref , Cetin Ihsan , Dokuyucu Recep
Published: October 2023

Background and Objectives: It is known that inflammatory processes play a role in the pathogenesis of autism spectrum disorder (ASD). It is also reported that immune activation induces the kynurenine pathway (KP), as known as the tryptophan destruction pathway. In our study, we aimed to investigate whether the serum levels of KP products and interleukin (IL)-6 activating indolamine 2–3 dioxygenase (IDO) enzyme are different in healthy developing children and children with ASD. Materials and Methods: Forty-three ASD children aged 2–9 were included in this study. Forty-two healthy developing children, similar to the patient group in terms of age and gender, were selected as the control group. Serum levels of kynurenic acid, kynurenine, quinolinic acid and IL-6 were analyzed using the ELISA method. ASD severity was evaluated with the Autism Behavior Checklist (ABC). Results: The mean age of children with ASD was 42.4 ± 20.5 months, and that of healthy controls was 48.1 ± 15.8 months. While the serum levels of kynurenic acid, kynurenine and interleukin-6 were higher in the group with ASD (p < 0.05), there was no significant difference (p > 0.05) in terms of the quinolinic acid level. There was no significant difference between the ABC total and subscale scores of children with ASD and biochemical parameters (p > 0.05). Conclusions: We conclude that these biomarkers must be measured in all ASD cases. They may be important for the diagnosis of ASD.
Stability of Filled PDMS Pervaporation Membranes in Bio-Ethanol Recovery from a Real Fermentation Broth
Authors: Van Goethem Cédric , Naik Parimal V. , Van de Velde Miet , Van Durme Jim , Verplaetse Alex , Vankelecom Ivo F. J.
Published: October 2023

Mixed matrix membranes (MMMs) have shown great potential in pervaporation (PV). As for many novel membrane materials however, lab-scale testing often involves synthetic feed solutions composed of mixed pure components, overlooking the possibly complex interactions and effects caused by the numerous other components in a real PV feed. This work studies the performance of MMMs with two different types of fillers, a core-shell material consisting of ZIF-8 coated on mesoporous silica and a hollow sphere of silicalite-1, in the PV of a real fermented wheat/hay straw hydrolysate broth for the production of bio-ethanol. All membranes, including a reference unfilled PDMS, show a declining permeability over time. Interestingly, the unfilled PDMS membrane maintains a stable separation factor, whereas the filled PDMS membranes rapidly lose selectivity to levels below that of the reference PDMS membrane. A membrane autopsy using XRD and SEM-EDX revealed an almost complete degradation of the crystalline ZIF-8 in the MMMs. Reference experiments with ZIF-8 nanoparticles in the fermentation broth demonstrated the influence of the broth on the ZIF-8 particles. However, the observed effects from the membrane autopsy could not exactly be replicated, likely due to distinct differences in conditions between the in-situ pervaporation process and the ex-situ reference experiments. These findings raise significant questions regarding the potential applicability of MOF-filled MMMs in real-feed pervaporation processes and, potentially, in harsh condition membrane separations in general. This study clearly confirms the importance of testing membranes in realistic conditions.
Ageing Organizations: Reviewing the Literature and Making a Few Recommendations for Human Resource Management
Authors: Salomão Filho Alfredo , Tillmanns Tanja , Corrigan Trudy
Published: October 2023

In an ageing society, organizations must consider the inclusion and effective management of older professionals if they wish to remain competitive. Besides having harmful and far-reaching effects on people’s health, age discrimination in the workplace leads to absenteeism, lower levels of productivity, and early retirement. Within such a context, this problematic organizational feature of most Western, now ageing, organizations and labour markets starts gaining more relevance. However, to date there has been little discussion, research, or policy development to ensure that older employees’ skills and capabilities are optimally put to use by organizations without the occurrence of ageism. We present the results of a systematic literature review based on 30 studies in the context of ageing organizations to make recommendations for human resource management. We suggest an approach to effectively manage intergenerational organizations, reducing the occurrence of age discrimination and its related consequences, as well as to achieve high employee engagement. Our review indicates that a strong ethics framework that is continuously followed, disseminated, and updated by management, together with a combination of efforts from all stakeholders, may accomplish the best results towards a culture that respects and values people of all ages at work, positively impacting on productivity.
The Influence of Interfacial Thermal Conductance on the Tensile Strength of a Sn-Mg Solder Alloy
Authors: Cruz Clarissa , Soares Thiago , Barros André , Garcia Amauri , Cheung Noé
Published: October 2023

Sn-Mg alloys are potential Pb-free solder options. However, their mechanical strength and interfacial characteristics with electronic substrates remain barely understood. This study focuses on the interfacial heat transfer aspects, microstructure, and tensile strength of a Sn-2.1wt.%Mg alloy. Samples with various thermal histories were produced using a directional solidification apparatus. In these experiments, a Sn-2.1wt.%Mg alloy was solidified on Cu and Ni substrates, which are of interest in the electronics industry. Mathematical modeling was then employed, allowing for the determination of the overall and interfacial heat transfer coefficients (hov, and hi, respectively). The results show that the Ni substrate exhibits higher interfacial thermal conductance with the Sn-2.1wt.%Mg alloy compared to the Cu substrate, as indicated by the higher hi profiles. This fact occurs mainly due to their metallurgical interaction, resulting in a stronger bond with the presence of Sn-Ni-rich intermetallics at the interface. Finally, experimental equations based on the Hall–Petch relationship are proposed to describe how the refinement of the fibrous spacing of the Mg2Sn interphase (λG) and an increase in hi enhance both yield and ultimate tensile strengths.
Lutein-Rich Beverage Alleviates Visual Fatigue in the Hyperglycemia Model of Sprague–Dawley Rats
Authors: Tang Qiong , Wei Sishan , He Xiangyi , Zheng Xiaodong , Tao Fei , Tu Pengcheng , Gao Bei
Published: October 2023

Asthenopia is a syndrome based on the symptoms of eye discomfort that has become a chronic disease that interferes with and harms people’s physical and mental health. Lutein is an internationally recognized “eye nutrient”, and studies have shown that it can protect the retina and relieve visual fatigue. In this study, lutein was extracted from marigold (Tagetes erecta L.) and saponified. The purified lutein concentration measured by HPLC was 50.12 mg/100 g. Then, purified lutein was modified to be water-soluble by nanoscale modification and microencapsulation technology. Water-soluble lutein was then mixed with a leaching solution of Chinese wolfberry and chrysanthemum to make a functional beverage. The effects of this beverage on hepatic antioxidant enzymes and the alleviation of visual fatigue in a rat model of diabetes were investigated for 4 weeks. Lutein intake of 0.72 (medium-lutein beverage group) and 1.44 mg/mL (high-lutein beverage group) relieved visual fatigue, ameliorated turbidity symptoms of impaired crystalline lenses, reduced hepatic MDA concentration, increased hepatic GSH concentration, and significantly increased the activities of the hepatic antioxidant enzymes SOD, CAT, GSH-Px, and GR in rats. These data suggest that a lutein-rich beverage is an effective and harmless way to increase the total anti-oxidation capacity of lenses and alleviate visual fatigue.
Real-Time Imaging of Plasmonic Concentric Circular Gratings Fabricated by Lens–Axicon Laser Interference Lithography
Authors: Mazloumi Mahyar , Sabat Ribal Georges
Published: October 2023

Concentric circular gratings are diffractive optical elements useful for polarization-independent applications in photonics and plasmonics. They are usually fabricated using a low-throughput and expensive electron beam lithography technique. In this paper, concentric circular gratings with selectable pitch values were successfully manufactured on thin films of azobenzene molecular glass using a novel laser interference lithography technique utilizing Bessel beams generated by a combined lens–axicon configuration. This innovative approach offers enhanced scalability and a simplified manufacturing process on larger surface areas compared to the previously reported techniques. Furthermore, the plasmonic characteristics of these concentric circular gratings were investigated using conventional spectrometric techniques after transferring the nanostructured patterns from azobenzene to transparent gold/epoxy thin films. In addition, the real-time imaging of surface plasmon resonance colors transmitted from the concentric circular gratings was obtained using a 45-megapixel digital camera. The results demonstrated a strong correlation between the real-time photographic technique and the spectroscopy measurements, validating the efficacy and accuracy of this approach for the colorimetric studying of surface plasmon resonance responses in thin film photonics.
Short-Term PE Generation Processes in the Soils of a Farmer Plots Network in the Madagascar Highlands: Actors and Drivers
Authors: Razanamalala Kanto , Razafimbelo Tantely , Maron Pierre-Alain , Ranjard Lionel , Chemidlin Prévost-Bouré Nicolas , Dequiedt Samuel , Becquer Thierry , Trap Jean , Blanchart Eric , Bernard Laetitia
Published: October 2023

Carbon sequestration in agricultural soils, through the accumulation of high-quality organic matter, represents great potential to mitigate climate change and simultaneously improve soil fertility. Such a challenge is particularly important and relevant in developing tropical countries like Madagascar, where soil carbon storage is vulnerable to climatic variations and where fertilization is generally applied through amendments in organic matter of various origins. The priming effect (PE) is considered here as the stimulation of the mineralization of soil organic matter (SOM) by a supply of fresh organic matter (FOM). PE results from different microbial processes driven by specific biotic and abiotic parameters. Depending on the processes involved, it has been suggested that PE could either counteract SOM accumulation or promote it. The objective of the present study was to explore the relationships between certain agricultural practices (type of crop, quality of fertilization, association with trees), the potential intensity of PE, as well as several abiotic (texture, quantity and quality SOM, nutrient enrichment) a1nd biotic (biomass and phylogenetic composition of microbial communities) factors which have been proposed in the literature as specific determinants of the different PE generation mechanisms. The soils for this study come from a network of farms in a commune in the Highlands of Madagascar. The PE, generated by a supply of 13C-enriched wheat straw, could not directly correlate with agricultural treatments. However, several indirect correlations could be found via several specific abiotic and microbial determinants that are discussed in terms of soil fertility restoration.
Gastrointestinal Pathogens in Multi-Infected Individuals: A Cluster Analysis of Interaction
Authors: Backhaus Joy , Frickmann Hagen , Hagen Ralf Matthias , Concha Gustavo , Molitor Ernst , Hoerauf Achim , Kann Simone
Published: October 2023

Indigenous people live in remote areas of Colombia. Multiple infections with bacteria, protozoa and/or helminths are common, as well as colonization in various forms. This study focused on the question of whether and to what extent various pathogens interact with each other. Therefore, a mathematical approach was retrospectively applied to PCR-based data of 244 stool samples, collected in two datasets. A stable cluster solution of the pathogens assessed was determined, and a unique configuration between Blastocystis hominis/Campylobacter spp./Giardia lamblia forming cluster 1 and Dientaemoeba fragilis was verified. A pathogen density-dependent interplay appeared between the B. hominis/Campylobacter spp./G. lamblia cluster, D. fragilis and Ascaris lumbricoides. The applied mathematical approach demonstrated that co-infections with parasites of questionable pathological relevance such as B. hominis and D. fragilis can be of diagnostic relevance due to their ability to promote or repress other pathogens. With the increasing availability of highly sensitive multiplexed molecular diagnostic approaches even in resource-limited settings, where multiple colonization of infection events with enteric pathogens in parallel are common, the importance of interpreting whole pathogen patterns rather than just individual pathogen detection may become more and more relevant.