bluuebunny/crossref_metadata_2025 huggingface dataset:
Persistent pain and long-term physical and mental conditions and their association with psychological well-being; data from 10,744 individuals from the Lolland-Falster health study
Authors: Tang Lars H , Andreasson Karen H , Thygesen Lau C , Jepsen Randi , Møller Anne , Skou Søren T
Published: November 2022

Introduction

Persistent pain (PP) and long-term conditions are all associated with psychological well-being. Less is known about their associations with reduced psychological well-being when co-occurring. We investigated how PP and long-term physical and mental conditions relate to psychological well-being when occurring together.

Method

Data collected in the Danish population-based Lolland-Falster Health study were used in this cross-sectional study. Participants aged ≥18 years completing questions concerning PP, long-term conditions and psychological well-being were included. PP and long-term conditions were defined as conditions lasting 6 months or longer. Psychological well-being was assessed by the World Health Organization Well-Being Index (WHO-5). Multiple linear regression investigated combinations of PP and physical and mental long-term conditions and their associations with WHO-5.

Results

Of 11,711 participants, 10,744 had available data. One third had PP ( n = 3250), while 6144 (57%), 213 (2%) and 946 (9%) reported having only physical conditions, only mental conditions or both, respectively. All combinations of PP and long-term conditions were negatively associated with WHO-5. PP in combination with mental (−23.1 (95% CI −28.3 to −17.8)) or both physical and mental conditions (−25.1 (−26.7 to −23.52) yielded the strongest negative associations. Two or more pain sites together with long-term physical and mental conditions was associated with a lower WHO-5 score (−6.2 (−8.9 to −3.5) compared to none or one pain site.

Conclusion

The presence of PP and long-term conditions, in particular mental conditions, were strongly associated with worse psychological well-being. This highlights the importance of assessing psychological well-being in individuals with PP and long-term conditions.
The identification and prediction of frailty based on Bayesian network analysis in a community-dwelling older population
Authors: Yuan Yin , Lin Siyang , Huang Xiaoming , Li Na , Zheng Jiaxin , Huang Feng , Zhu Pengli
Published: November 2022

Background

We have witnessed frailty, which characterized by a decline in physiological reserves, become a major public health issue in older adults. Understanding the influential factors associated with frailty may help prevent or if possible reverse frailty. The present study aimed to investigate factors associated with frailty status and frailty transition in a community-dwelling older population.

Methods

A prospective cohort study on community-dwelling subjects aged ≥ 60 years was conducted, which was registered beforehand (ChiCTR 2,000,032,949). Participants who had completed two visits during 2020–2021 were included. Frailty status was evaluated using the Fried frailty phenotype. The least absolute shrinkage and selection operator (LASSO) regression was applied for variable selection. Bayesian network analysis with the max-min hill-climbing (MMHC) algorithm was used to identify factors related to frailty status and frailty transition.

Results

Of 1,981 subjects at baseline, 1,040 (52.5%) and 165 (8.33%) were classified as prefrailty and frailty. After one year, improved, stable, and worsening frailty status was observed in 460 (35.6%), 526 (40.7%), and 306 (23.7%) subjects, respectively. Based on the variables screened by LASSO regression, the Bayesian network structure suggested that age, nutritional status, instrumental activities of daily living (IADL), balance capacity, and social support were directly related to frailty status. The probability of developing frailty is 14.4% in an individual aged ≥ 71 years, which increases to 20.2% and 53.2% if the individual has balance impairment alone, or combined with IADL disability and malnutrition. At a longitudinal level, ADL/IADL decline was a direct predictor of worsening in frailty state, which further increased the risk of hospitalization. Low high-density lipoprotein cholesterol (HDL-C) and diastolic blood pressure (DBP) levels were related to malnutrition, and further had impacts on ADL/IADL decline, and ultimately led to the worsening of the frailty state. Knowing the status of any one or more of these factors can be used to infer the risk of frailty based on conditional probabilities.

Conclusion

Older age, malnutrition, IADL disability, and balance impairment are important factors for identifying frailty. Malnutrition and ADL/IADL decline further predict worsening of the frailty state.
Evaluation of multi-assay algorithms for cross-sectional HIV incidence estimation in settings with universal antiretroviral treatment
Authors: Grant-McAuley Wendy , Laeyendecker Oliver , Monaco Daniel , Chen Athena , Hudelson Sarah E. , Klock Ethan , Brookmeyer Ron , Morrison Douglas , Piwowar-Manning Estelle , Morrison Charles S. , Hayes Richard , Ayles Helen , Bock Peter , Kosloff Barry , Shanaube Kwame , Mandla Nomtha , van Deventer Anneen , Ruczinski Ingo , Kammers Kai , Larman H. Benjamin , Eshleman Susan H.
Published: November 2022

Background

Multi-assay algorithms (MAAs) are used to estimate population-level HIV incidence and identify individuals with recent infection. Many MAAs use low viral load (VL) as a biomarker for long-term infection. This could impact incidence estimates in settings with high rates of early HIV treatment initiation. We evaluated the performance of two MAAs that do not include VL.

Methods

Samples were collected from 219 seroconverters (infected < 1 year) and 4376 non-seroconverters (infected > 1 year) in the HPTN 071 (PopART) trial; 28.8% of seroconverter samples and 73.2% of non-seroconverter samples had VLs ≤ 400 copies/mL. Samples were tested with the Limiting Antigen Avidity assay (LAg) and JHU BioRad-Avidity assays. Antibody reactivity to two HIV peptides was measured using the MSD U-PLEX assay. Two MAAs were evaluated that do not include VL: a MAA that includes the LAg-Avidity assay and BioRad-Avidity assay (LAg + BR) and a MAA that includes the LAg-Avidity assay and two peptide biomarkers (LAg + PepPair). Performance of these MAAs was compared to a widely used MAA that includes LAg and VL (LAg + VL).

Results

The incidence estimate for LAg + VL (1.29%, 95% CI: 0.97–1.62) was close to the observed longitudinal incidence (1.34% 95% CI: 1.17–1.53). The incidence estimates for the other two MAAs were higher (LAg + BR: 2.56%, 95% CI 2.01–3.11; LAg + PepPair: 2.84%, 95% CI: 1.36–4.32). LAg + BR and LAg + PepPair also misclassified more individuals infected > 2 years as recently infected than LAg + VL (1.2% [42/3483 and 1.5% [51/3483], respectively, vs. 0.2% [6/3483]). LAg + BR classified more seroconverters as recently infected than LAg + VL or LAg + PepPair (80 vs. 58 and 50, respectively) and identified ~ 25% of virally suppressed seroconverters as recently infected.

Conclusions

The LAg + VL MAA produced a cross-sectional incidence estimate that was closer to the longitudinal estimate than two MAAs that did not include VL. The LAg + BR MAA classified the greatest number of individual seroconverters as recently infected but had a higher false recent rate.
A deep learning approach for automated diagnosis of pulmonary embolism on computed tomographic pulmonary angiography
Authors: Ajmera Pranav , Kharat Amit , Seth Jitesh , Rathi Snehal , Pant Richa , Gawali Manish , Kulkarni Viraj , Maramraju Ragamayi , Kedia Isha , Botchu Rajesh , Khaladkar Sanjay
Published: November 2022

Background

Computed tomographic pulmonary angiography (CTPA) is the diagnostic standard for confirming pulmonary embolism (PE). Since PE is a life-threatening condition, early diagnosis and treatment are critical to avoid PE-associated morbidity and mortality. However, PE remains subject to misdiagnosis.

Methods

We retrospectively identified 251 CTPAs performed at a tertiary care hospital between January 2018 to January 2021. The scans were classified as positive (n = 55) and negative (n = 196) for PE based on the annotations made by board-certified radiologists. A fully anonymized CT slice served as input for the detection of PE by the 2D segmentation model comprising U-Net architecture with Xception encoder. The diagnostic performance of the model was calculated at both the scan and the slice levels.

Results

The model correctly identified 44 out of 55 scans as positive for PE and 146 out of 196 scans as negative for PE with a sensitivity of 0.80 [95% CI 0.68, 0.89], a specificity of 0.74 [95% CI 0.68, 0.80], and an accuracy of 0.76 [95% CI 0.70, 0.81]. On slice level, 4817 out of 5183 slices were marked as positive for the presence of emboli with a specificity of 0.89 [95% CI 0.88, 0.89], a sensitivity of 0.93 [95% CI 0.92, 0.94], and an accuracy of 0.89 [95% CI 0.887, 0.890]. The model also achieved an AUROC of 0.85 [0.78, 0.90] and 0.94 [0.936, 0.941] at scan level and slice level, respectively for the detection of PE.

Conclusion

The development of an AI model and its use for the identification of pulmonary embolism will support healthcare workers by reducing the rate of missed findings and minimizing the time required to screen the scans.
Maternal psychological distress in the early postpartum period during COVID-19 pandemic: a pilot study
Authors: Wang Yao-Sheng , Cheng Wen-Hsin , Chen I-Lun , Huang Hsin-Chun
Published: November 2022

Background

The coronavirus disease 2019 infection (COVID-19) pandemic is a new global outbreak disease. According to the Taiwan Centers for Diseases Control statement, hospitals had to change their corresponding measures to prevent the spread of COVID-19. The frequency of parental visits to the special care nursery was reduced from three times to once daily. Visiting was not permitted from April 4 to May 10, 2020, and rooming-in with healthy neonates was discontinued, which could increase maternal postpartum distress. Therefore, this study was conducted to determine whether COVID-19 prevention increased maternal psychological distress.

Methods

This prospective study used convenience sampling to enroll healthy mothers who had just delivered via normal spontaneous delivery. Based on the neonates’ status and visiting times, mothers were grouped into no-rooming-in, rooming-in, no-visiting, and one-visit/day groups. Mothers’ baseline characteristics were compared using the Chi-square or Fisher's exact test and t-test. Salivary cortisol levels and scores of Chinese versions of the Perceived Stress Scale (PSS) and State-Trait Anxiety Inventory were evaluated on postpartum days 1 and 3 and analyzed by one-way analysis of variance and a paired t-test.

Results

There were 16, 58, 28, and 47 women categorized as no-rooming-in, rooming-in, no-visit, and one-visit/day groups, respectively. No significant differences were found between groups in mothers’ baseline characteristics and postpartum salivary cortisol levels. The PSS on day 3 was significantly higher than on day 1 in every group (p < 0.001). The PSS increasing trend in the no-rooming-in group was significantly greater than that in the no-visit group (p = 0.02) and significantly greater in the rooming-in group than that in the one-visit/day group (p = 0.001).

Conclusion

Postpartum stress increased for all mothers and was an even more significant response to the COVID-19 pandemic than the stress associated with neonates’ hospitalization.
Women’s perceptions of cardiovascular risk after preeclampsia: a qualitative study
Authors: Nielsen Lene Musfelt , Hauge Maria Guldbrandt , Ersbøll Anne S. , Johansen Marianne , Linde Jesper James , Damm Peter , Nielsen Karoline Kragelund
Published: November 2022

Background

Preeclampsia is associated with increased risk of cardiovascular disease later in life, but studies suggest that women with previous preeclampsia are not aware of this. Little is known about how these women perceive the condition and the associated long-term risks. We examined the experiences and perceptions of preeclampsia and the increased risk of cardiovascular disease (CVD) later in life among Danish women with previous preeclampsia and their attitudes towards CVD risk screening.

Methods

Ten individual semi-structured interviews were conducted with women with previous preeclampsia. Data were analysed using thematic analysis.

Results

We identified six themes: 1) Experiences and perceptions of being diagnosed with preeclampsia, 2) Awareness about increased risk of CVD later in life, 3) Knowledge as a precondition for action, 4) The perception of CVD risk as being modifiable, 5) Motivators for and barriers to a healthy lifestyle, and 6) Screening for CVD. Awareness of the severity of preeclampsia was limited prior to being diagnosed. Particularly among those with few or no symptoms, preeclampsia was perceived as a non-severe condition, which was further reinforced by the experience of having received very little information. Nonetheless, some women were shocked by the diagnosis and feared for the health of the offspring. Many women also experienced physical and psychological consequences of preeclampsia. Awareness of the increased risk of later CVD was lacking; yet, when informed, the women considered this to be essential knowledge to be able to act accordingly. The risk of future CVD was perceived to be partly modifiable with a healthy lifestyle, and the women expressed a need for counselling on appropriate lifestyle changes to reduce CVD risk. Other factors were also mentioned as imperative for lifestyle changes, including social support. The women were generally positive towards potential future screening for CVD because it could provide them with information about their health condition.

Conclusions

After preeclampsia, women experienced a lack of knowledge on preeclampsia and the increased risk of CVD later in life. Improved information and follow-up after preeclampsia, including guidance on CVD risk reduction and support from health professionals and family, are warranted.
An evaluation of the efficacy of a supplemental computer-based tutorial to enhance the informed consent process for cataract surgery: an exploratory randomized clinical study
Authors: Ullrich Marlies , Findl Oliver , Kefer Katharina , Döller Birgit , Varsits Ralph , Hienert Julius , Hirnschall Nino
Published: November 2022

Background

To assess whether informing patients with a computer-based tutorial in addition to standard informed consent influences the patient’s attitude towards surgery and increases patient’s knowledge.

Methods

In this prospective, exploratory, randomized clinical study, patients scheduled for their first eye cataract surgery were randomly allocated to two groups, receiving standard face-to-face informed consent (control group) or additionally using an interactive computer-based tool (CatInfo) containing an audiovisual presentation about cataract and its treatment (study group). Cataract-related knowledge and decisional confidence (decisional conflict scale (DCS)) were assessed as well as one-month postoperatively decisional regret (decision regret scale (DRS)) and willingness to exchange face-to-face discussion time for the use of such a tool.

Results

The study comprised 134 patients, 64 patients in the study group and 70 in the control group. Patients in the study group answered more questions correctly, 16.3 ± 2.0 (median 16.5, 11.0–19.0) versus 15.5 ± 1.9 (median 16.0, 8.0–19.0; p = 0.01). Patients showed a high decisional confidence with a study group mean DCS score of 92.4 ± 9.8 (median 96.9, 65.6–100) and control group score of 91.6 ± 10.9 (median 95.3, 43.3–100; p = 0.52). Mean DRS score in the study group was 2.5 ± 8.0 (median 0, 0–40) and 4.3 ± 12.5 (median 0, 0–75) in the control group (p = 0.14). Of study group patients 23 (67.6%) were willing to trade time, on average 158 ± 180 s (median 120 s, 45–900). Satisfaction with the tool was high with a mean of 9.1 ± 1.3 out of 10 (median 9.7, 5.0–10).

Conclusions

Cataract-related knowledge was generally good, with slightly higher scores in the study group. In both groups, decisional confidence was high and regret after surgery was low. A tendency towards slightly higher decisional confidence and lower regret was found in the study group, although these differences were not statistically significant. Additional use of an interactive computer-based tool may prove useful in the informed consent process in a high-volume cataract outpatient setting.

Trial registration

ClinicalTrials.gov, NCT04975126. Retrospectively registered – July 23, 2021.
Olfactory mucosa tissue-derived mesenchymal stem cells lysate ameliorates LPS-induced acute liver injury in mice
Authors: Wang Zhe , Zhang XingXing , Qi Liuyao , Feng Wenjing , Gu Yahan , Ding Yuting
Published: November 2022

Background

Acute liver injury (ALI) induced by sepsis seriously endangers the health of human beings every year. Mesenchymal stem cells (MSCs) lysate containing various regulators had a positive effect on anti-inflammation, hoping to provide a promising strategy in ALI.

Methods

Olfactory mucosa-derived mesenchymal stem cells (OM-MSCs) were extracted and identified. The collected OM-MSCs were prepared after repeated freeze–thaw in phosphate buffer solution (PBS). Then, OM-MSCs lysate was filtered for future experiments. To understand the composes of OM-MSCs clearly, we detected the components of OM-MSCs lysate by western blotting.In vitro, OM-MSCs lysate was applied to evaluate the effects on normal human liver cells (LO-2) under stimulation of LPS. Lipopolysaccharide (LPS) was also injected intraperitoneally to build ALI model in mice. We further assessed the anti-inflammatory capacity of OM-MSCs lysate on ALIin vivoby aminotransferase determination, pathology observation, and immunohistochemical staining. Moreover, the immunoblot technique was performed to recognize the changes in inflammatory factors and related proteins.

Results

In this study, we found that OM-MSCs lysate could protect structure effectively, improve the plasma aminotransferases, diminish inflammation by releasing interleukin-10 (IL-10) and transforming growth factor-beta (TGF-β). A significant decrease in tumor necrosis factor-α (TNF-α) also occurred under the treatment of OM-MSCs lysate. In addition, trophic factors originating from OM-MSCs lysate provided a supportive micro-environment for liver recovery. Especially, up-expression of vascular endothelial growth factor (VEGF)in vivorevealed that OM-MSCs might have a great potential for healing.

Conclusions

Our results demonstrated that OM-MSCs lysate could alleviate LPS-induced ALI via decreasing inflammatory cytokines and promoting recovery.
Why a large percentage of Tunisian women aged 40 years and more has a reduced forced vital capacity? The implication of parity
Authors: Saad Helmi Ben
Published: November 2022

The investigation of the link between reduced forced vital capacity (FVC) and risk factors and health variables in women aged ≥ 40 years is encouraged since a reduced FVC was related to all-cause mortality. The high frequency of women with a reduced FVC, observed in some studies, could be related to the impacts of parity on lung. In the literature, the association between parity and health consequences is discussed in terms of “selection pressure”, and the trade-off between longevity and fertility described by scientists is termed the “longevity determination” or “biological warranty period”. The respiratory system could be influenced by parity. Above all, it is the respiratory system, who endures the repercussions of the numerous physio-pathological experiences of the woman life. The probable effects of parity on lung function data, including FVC, make parity a key predictor to be stressed and evaluated. Parity is a promising original direction for physiological and pathophysiological research, particularly for low- and lower-middle- income countries. Thus, upcoming epidemiological and clinical studies of lung function data in women would need to include information about their parity status.
Self-efficacy and positive thinking as predictors of health-related quality of life in women with stress urinary incontinence
Authors: Chien Ching Hui , Huang Xuan Yi , Hsu Shu Pen , Yen Yi Hua , Pan Hun Shan , Yen Feng Chu
Published: November 2022

Background

Stress urinary incontinence (SUI), which causes involuntarily leakage of urine, has an impact on many women and may affect self-efficacy, which, in turn, can lead to poor health-related quality of life (QOL). This study aimed to explore the effects of sociodemographic and health information, symptom distress, self-efficacy, and positive thinking on the health-related QOL (general QOL and urinary incontinence-specific QOL) of women with SUI.

Methods

A cross-sectional study design was used. Women with SUI were recruited from the obstetrics and gynecology outpatient department and urodynamics examination room of a hospital by convenience sampling from August 2021 to March 2022. Participants were surveyed on the following questionnaires: Urogenital Distress Inventory, Geriatric Self-efficacy Index for Urinary Incontinence, Positive Thinking Scale, 12-Item Short-Form Health Survey (SF-12), and Incontinence Impact Questionnaire Short Form.

Results

Participants (N = 135) had a mean age of 53.76 years old. The mean SF-12 physical component summary score was 48.48 (physical QOL), and the mental component summary score was 46.56 (mental QOL). The urinary incontinence-specific QOL score was 16.01. Women with greater positive thinking and higher self-efficacy for urinary incontinence had better physical and mental QOL. Women with less symptom distress of urinary incontinence and higher self-efficacy for urinary incontinence had better urinary incontinence-specific QOL.

Conclusion

The health-related QOL of women with SUI is affected by many factors, including positive thinking, self-efficacy, and symptom distress. Healthcare professionals can provide multifaceted programs to improve the health-related QOL of women with SUI.
Health promotion and disease prevention in the education of health professionals: a mapping of European educational programmes from 2019
Authors: Patja Kristiina , Huis in ‘t Veld Tessa , Arva Dorottya , Bonello Marjorie , Orhan Pees Rana , Soethout Marc , van der Esch Martin
Published: November 2022

Background

Health professionals face barriers in carrying out effective health promotion and disease prevention. To indicate what are the needs for curriculum development in educational programmes, this study aims to provide an overview of how various health professionals are currently trained in health promotion and disease prevention at different educational levels.

Methods

In 2019, a descriptive mapping exercise was performed focusing on European programmes for different health and healthcare professionals at the three levels of education (undergraduate, postgraduate, and continuous professional development [CPD]). Data were collected by a self-developed online survey that was distributed using a modified snowball method.

Results

A total of 186 educational programmes of 17 different health professionals were analysed, implemented in 31 countries (60% were undergraduate, 30% postgraduate and 10% CPD programmes). Nearly all programmes indicated that expected outcomes were defined on knowledge (99%), skills (94%) and behaviours/attitudes (89%) regarding health promotion and disease prevention. A multidisciplinary approach was reported to be applied by 81% of the programmes. Traditional teaching methods such as lectures (97%) and assignments (81%) were dominant, while e-learning was less frequently used (46%). Digitalization in health promotion and digital health coaching were the least addressed topics in most programmes.

Conclusions

Health promotion and disease prevention are reported at all surveyed levels of education for a broad spectrum of health professionals. Educational programmes cover contents on knowledge, skills, and behaviours. There is a need for capacity building and joint development in health promotion education. Specifically, there is a need to include digitalisation and novel teaching in the educational programmes of health promotion and disease prevention.
Patient satisfaction with pharmacist-administered COVID-19 vaccines in Poland: a survey study in the vaccination centres context
Authors: Merks Piotr , Kowalczuk Anna , Wong Alexandre , Chung Kevin , Religioni Urszula , Świetlik Dariusz , Rotmans-Plagens Katarzyna , Cameron Jameason , Sola Katarina Fehir , Kazmierczak Justyna , Blicharska Eliza , Vaillancourt Regis , Neumann-Podczaska Agnieszka
Published: November 2022

Background

Since 2021, pharmacists in Poland have been authorised to administer vaccinations against COVID-19, which is of particular significance in the efforts towards preventing the spread of the pandemic. The primary objective of this study was to evaluate the patients’ satisfaction with delivering vaccinations through national vaccination centres.

Methods

This study was conducted in 2021. The research tool was an anonymous questionnaire distributed to patients after vaccination. The questionnaire was developed specifically for the purpose of the study. Ultimately, 628 patients participated in this study.

Results

Nearly 97% of the respondents agreed that the administration of vaccinations by pharmacists had been convenient, and pharmacists possessed the relevant skills to provide this service. Almost 90% of the respondents expressed their readiness to be vaccinated by pharmacists again. Nearly all the respondents indicated that pharmacists should also provide other vaccinations.

Conclusions

Patients in Poland have a positive attitude toward vaccinations administered by pharmacists in national vaccination centres.
Garvicin Q: characterization of biosynthesis and mode of action
Authors: Desiderato Christian K. , Hasenauer Katharina M. , Reich Sebastian J. , Goldbeck Oliver , Holivololona Lalaina , Ovchinnikov Kirill V. , Reiter Alexander , Oldiges Marco , Diep Dzung B. , Eikmanns Bernhard J. , Riedel Christian U.
Published: November 2022

Bacteriocins are ribosomally synthesized antimicrobial peptides, that either kill target bacteria or inhibit their growth. Bacteriocins are used in food preservation and are of increasing interest as potential alternatives to conventional antibiotics. In the present study, we show that Lactococcus petauri B1726, a strain isolated from fermented balsam pear, produces a heat-stable and protease-sensitive compound. Following genome sequencing, a gene cluster for production of a class IId bacteriocin was identified consisting of garQ (encoding for the bacteriocin garvicin Q), garI (for a putative immunity protein), garC, and garD (putative transporter proteins). Growth conditions were optimized for increased bacteriocin activity in supernatants of L. petauri B1726 and purification and mass spectrometry identified the compound as garvicin Q. Further experiments suggest that garvicin Q adsorbs to biomass of various susceptible and insusceptible bacteria and support the hypothesis that garvicin Q requires a mannose-family phosphotransferase system (PTSMan) as receptor to kill target bacteria by disruption of membrane integrity. Heterologous expression of a synthetic garQICD operon was established in Corynebacterium glutamicum demonstrating that genes garQICD are responsible for biosynthesis and secretion of garvicin Q. Moreover, production of garvicin Q by the recombinant C. glutamicum strain was improved by using a defined medium yet product levels were still considerably lower than with the natural L. petauri B1726 producer strain.

Collectively, our data identifies the genetic basis for production of the bacteriocin garvicin Q by L. petauri B1726 and provides insights into the receptor and mode of action of garvicin Q. Moreover, we successfully performed first attempts towards biotechnological production of this interesting bacteriocin using natural and heterologous hosts.
VECTRON™ T500, a new broflanilide insecticide for indoor residual spraying, provides prolonged control of pyrethroid-resistant malaria vectors
Authors: Govoetchan Renaud , Fongnikin Augustin , Syme Thomas , Small Graham , Gbegbo Martial , Todjinou Damien , Rowland Mark , Nimmo Derric , Padonou Germain Gil , Ngufor Corine
Published: November 2022

Background

Broflanilide is a newly discovered insecticide with a novel mode of action targeting insect γ-aminobutyric acid receptors. The efficacy of VECTRON™ T500, a wettable powder formulation of broflanilide, was assessed for IRS against wild pyrethroid-resistant malaria vectors in experimental huts in Benin.

Methods

VECTRON™ T500 was evaluated at 100 mg/m2 in mud and cement-walled experimental huts against wild pyrethroid-resistant Anopheles gambiae sensu lato (s.l.) in Covè, southern Benin, over 18 months. A direct comparison was made with Actellic® 300CS, a WHO-recommended micro-encapsulated formulation of pirimiphos-methyl, applied at 1000 mg/m2. The vector population at Covè was investigated for susceptibility to broflanilide and other classes of insecticides used for vector control. Monthly wall cone bioassays were performed to assess the residual efficacy of VECTRON™ T500 using insecticide susceptible An. gambiae Kisumu and pyrethroid-resistant An. gambiae s.l. Covè strains. The study complied with OECD principles of good laboratory practice.

Results

The vector population at Covè was resistant to pyrethroids and organochlorines but susceptible to broflanilide and pirimiphos-methyl. A total of 23,171 free-flying wild pyrethroid-resistant female An. gambiae s.l. were collected in the experimental huts over 12 months. VECTRON™ T500 induced 56%-60% mortality in wild vector mosquitoes in both cement and mud-walled huts. Mortality with VECTRON™ T500 was 62%-73% in the first three months and remained > 50% for 9 months on both substrate-types. By comparison, mortality with Actellic® 300CS was very high in the first three months (72%-95%) but declined sharply to < 40% after 4 months. Using a non-inferiority margin defined by the World Health Organization, overall mortality achieved with VECTRON™ T500 was non-inferior to that observed in huts treated with Actellic® 300CS with both cement and mud wall substrates. Monthly in situ wall cone bioassay mortality with VECTRON™ T500 also remained over 80% for 18 months but dropped below 80% with Actellic® 300CS at 6–7 months post spraying.

Conclusion

VECTRON™ T500 shows potential to provide substantial and prolonged control of malaria transmitted by pyrethroid-resistant mosquito vectors when applied for IRS. Its addition to the current list of WHO-approved IRS insecticides will provide a suitable option to facilitate rotation of IRS products with different modes of action.
Retraction Note to: LncRNA PVT1 triggers Cyto-protective autophagy and promotes pancreatic ductal adenocarcinoma development via the miR-20a-5p/ULK1 Axis
Authors: Huang Fengting , Chen Wenying , Peng Juanfei , Li Yuanhua , Zhuang Yanyan , Zhu Zhe , Shao Chunkui , Yang Wanling , Yao Herui , Zhang Shineng
Published: November 2022

This article has been retracted. Please see the Retraction Notice for more detail: https://doi.org/10.1186/s12943-018-0845-6.
Detection of cytomegalovirus (CMV) by digital PCR in stool samples for the non-invasive diagnosis of CMV gastroenteritis
Authors: Gu Jia , Ji Hongyan , Liu Tongyuan , Chen Caixia , Zhao Siye , Cao Yang , Wang Na , Xiao Min , Chen Liting , Cai Haodong
Published: November 2022

Background

CMV gastroenteritis is common in patients receiving allogeneic hematopoietic stem cell transplantation and it is difficult to distinguish from acute graft-versus-host disease (aGvHD), which has very similar symptoms but needs quite different treatment. CMV gastroenteritis is caused by local infection or reactivation of CMV in the gastrointestinal tract while aGvHD is due to immune rejection. The gold standard of diagnosis of CMV gastroenteritis and aGvHD is gastrointestinal biopsy under endoscopy, which is invasive and can potentially lead to severe side effects. Stool samples testing with quantitative polymerase chain reaction (qPCR) may be an alternative, while the application in trace level measurements and precision are not all satisfactory enough in reported research.

Methods

In this study, we designed a novel method that extracted the cell free DNA (cfDNA) from the fecal supernatant to perform digital PCR (dPCR) for the detection of CMV, analyzed the performance and compared it with the total DNA extracted by the current procedure.

Results

Twenty-two paired stool samples using two DNA extraction methods proved that the cfDNA extraction method had markedly higher DNA concentrations and control gene copy number, suggesting that cfDNA may be more informative and more useful for the detection of CMV DNA segment. The dPCR approach in detecting CMV DNA segment also exhibit good linearity (R2 = 0.997) and higher sensitivity (limit of detection at 50% was 3.534 copies/μL). Eighty-two stool samples from 44 immunocompromised patients were analyzed, CMV-positive rate was 28%, indicating that more than one-quarter of the gastrointestinal symptoms within these patients may be caused by CMV infection or reactivation.

Conclusion

The combined results suggest that detection of CMV by dPCR in cfDNA of stool supernatant is a powerful method to identify CMV gastroenteritis and helps in clinical treatment decision making.
Retained introns in long RNA-seq reads are not reliably detected in sample-matched short reads
Authors: David Julianne K. , Maden Sean K. , Wood Mary A. , Thompson Reid F. , Nellore Abhinav
Published: November 2022

Background

There is growing interest in retained introns in a variety of disease contexts including cancer and aging. Many software tools have been developed to detect retained introns from short RNA-seq reads, but reliable detection is complicated by overlapping genes and transcripts as well as the presence of unprocessed or partially processed RNAs.

Results

We compared introns detected by 8 tools using short RNA-seq reads with introns observed in long RNA-seq reads from the same biological specimens. We found significant disagreement among tools (Fleiss’ $$\kappa = 0.113$$

κ
=
0.113

) such that 47.7% of all detected intron retentions were not called by more than one tool. We also observed poor performance of all tools, with none achieving an F1-score greater than 0.26, and qualitatively different behaviors between general-purpose alternative splicing detection tools and tools confined to retained intron detection.

Conclusions

Short-read tools detect intron retention with poor recall and precision, calling into question the completeness and validity of a large percentage of putatively retained introns called by commonly used methods.
Comparative evaluation of different versions of exposure-free mosquito electrocuting traps and barrier screen trap for monitoring outdoor densities and biting time phenotypes by malaria and filariasis vectors in Tanzania
Authors: Githu Victoria , Baravuga Maneno E. , Mbarawa Asiya , Msuya Hajirani M. , Mlacha Yeromin P. , Chaki Prosper P. , Kiware Samson , Mirzai Nosrat , Ferguson Heather M. , Govella Nicodem J.
Published: November 2022

Background

Estimating human exposure to mosquito vectors is crucial for the prediction of malaria transmission and intervention impact. The human landing catch method is frequently used to directly measure estimate exposure rates; however, there has been an increasing shift from this method to exposure-free alternatives, such as the mosquito electrocuting traps (MET) and other approaches. While these latter methods can provide robust and representative values of human exposure and mosquito density, they often still require a human volunteer, which poses logistical challenges. Additionally, in the case of the MET, the early MET prototype (METe) required human volunteers to wear protective clothing that could be uncomfortable. We investigated two alternative trapping approaches to address these challenges by comparing the performance of the METe prototype to: (i) a modified caged MET prototype that offers full protection to users (METc) and (ii) a barrier screen trap (BST) designed to passively sample (host-seeking and blood-fed) mosquitoes outdoors without requiring a human participant.

Methods

The relative performance of the METe, METc and BST were evaluated in a 3 × 3 Latin square field experiment design conducted in south-eastern Tanzania over 12 nights of sampling. The outcomes of interest were the nightly catch of mosquitoes and biting time estimates.

Results

The METc and BST caught similar numbers of An. arabiensis as the METe (relative ratio [RR] = 0.76, 95% confidence interval [CI]: 0.42–1.39, P = 0.38 and RR = 1.13, 95% CI: 0.63–2.04, P = 0.69, respectively). Similarly, the METc and BST caught similar numbers of Culex spp. as the METe (RR = 0.87, 95% CI: 0.62–1.22, P = 0.42 and RR = 0.80, 95% CI: 0.57–1.12, P = 0.199, respectively). All three trapping methods indicated a similar pattern of biting activity by An. arabiensis and Culex spp., characterized by biting starting in the early evening (18:00–22:00), peaking when people are typically sleeping (22:00–05:00) and dropping off drastically toward the morning (05:00–07:00).

Conclusions

The modifications made to the METe design to improve user comfort and remove the need for protective clothing did not result in an underestimation of mosquito vector abundance nor misrepresentation of their biting time pattern. We recommend the METc for use over the METe design. Similarly, the BST demonstrated potential for monitoring malaria and filariasis vector densities in Tanzania.

Graphical Abstract
Distinctive alterations in the functional anatomy of the cerebral cortex in pain-sensitized osteoarthritis and fibromyalgia patients
Authors: Pujol Jesus , Blanco-Hinojo Laura , Doreste Andrea , Ojeda Fabiola , Martínez-Vilavella Gerard , Pérez-Sola Víctor , Deus Joan , Monfort Jordi
Published: November 2022

Background

Pain-sensitized osteoarthritis and fibromyalgia patients characteristically show nociceptive system augmented responsiveness as a common feature. However, sensitization can be originally related to the peripheral injury in osteoarthritis patients, whereas pain and bodily discomfort spontaneously occur in fibromyalgia with no apparent origin. We investigated the distinct functional repercussion of pain sensitization in the cerebral cortex in both conditions.

Methods

Thirty-one pain-sensitized knee osteoarthritis patients and 38 fibromyalgia patients were compared with matched control groups. And new samples of 34 sensitized knee osteoarthritis and 63 fibromyalgia patients were used to directly compare each condition. A combined measure of local functional connectivity was estimated to map functional alterations in the cerebral cortex at rest.

Results

In osteoarthritis, weaker local connectivity was identified in the insula, which is a cortical area processing important aspects of the brain response to painful stimulation. In contrast, fibromyalgia patients showed weaker connectivity in the sensorimotor cortex extensively affecting the cortical representation of the body.

Conclusions

In osteoarthritis, weaker insular cortex connectivity is compatible with reduced neural activity during metabolic recovery after repeated activation. In the fibromyalgia neurophysiological context, weaker connectivity may better express both reduced neural activity and increased excitability, particularly affecting the sensorimotor cortex in patients with spontaneous body pain. Such a combination is compatible with a central gain enhancement mechanism, where low sensory tolerance results from the over-amplification of central sensory reception to compensate a presumably weak sensory input. We propose that deficient proprioception could be a factor contributing to weak sensory input.
RENEWAL: REpurposing study to find NEW compounds with Activity for Lewy body dementia—an international Delphi consensus
Authors: O’Brien John T. , Chouliaras Leonidas , Sultana Janet , Taylor John-Paul , Ballard Clive ,   on behalf of the RENEWAL Study Group, Aarsland Dag , Blanc Frederic , Boeve Bradley , Brooks David J. , Chaudhuri K. Ray , Cummings Jeffrey , Feldman Howard H. , Flicker Leon , Galvin James E. , Grosset Donald G. , Ikeda Manabu , Kohlhaas Susan , Lawlor Brian , Lemstra Afina W. , Leroi Iracema , Londos Elisabet , Leverenz James B. , Lewis Simon , McKeith Ian , Mills Roger , Oakley Richard , Richardson Jill , Sabbagh Marwan , Skidmore John , Svennigsson Per , Tiraboschi Pietro , Weintraub Daniel , Walker Zuzana , Watson Rosie , Weil Rimona S. , Williams-Gray Caroline H. , Yarnall Alison
Published: November 2022

Drug repositioning and repurposing has proved useful in identifying new treatments for many diseases, which can then rapidly be brought into clinical practice. Currently, there are few effective pharmacological treatments for Lewy body dementia (which includes both dementia with Lewy bodies and Parkinson’s disease dementia) apart from cholinesterase inhibitors. We reviewed several promising compounds that might potentially be disease-modifying agents for Lewy body dementia and then undertook an International Delphi consensus study to prioritise compounds. We identified ambroxol as the top ranked agent for repurposing and identified a further six agents from the classes of tyrosine kinase inhibitors, GLP-1 receptor agonists, and angiotensin receptor blockers that were rated by the majority of our expert panel as justifying a clinical trial. It would now be timely to take forward all these compounds to Phase II or III clinical trials in Lewy body dementia.
On integer parts of the reciprocal remainders of some sums
Authors: Stević Stevo
Published: November 2022

We present generalizations of some results on the integer parts of the reciprocal remainders of the zeta function $\zeta (s)$
ζ
(
s
)
with $s=2$
s
=
2
and $s=3$
s
=
3
, and a very short and elegant proof of a recent result on the integer parts of the reciprocal remainders of the series $\zeta (3)$
ζ
(
3
)
. We also give some historical and theoretical remarks to problems of this type, conduct some analyses, and make some connections with the theory of linear difference equations with constant coefficients.
Relationship between body composition indices and changes in body temperature due to hot pack use
Authors: Kominami Kazuyuki , Sato Kazunari , Takahashi Naoaki
Published: November 2022

Background

Hot pack application is used to reduce pain and muscle stiffness at the treated site. However, the effects of hot pack application on the whole body have not been clarified. We investigated the relationship between body composition indices and the hot pack-induced increase in body temperature.

Methods

We recruited 17 healthy men (age, 22.0 ± 3.3 years) who participated in the study on five different days and applied “dry” hot packs at four different sites (the most frequently used sites): right shoulder, lower back, both popliteal areas, and lower back plus popliteal areas. The study protocol involved the measurement of body composition followed by 10 min of bed rest, 15 min of warming with a hot pack, and 20 min of subsequent rest. Heart rate and body temperature were measured continuously, and blood pressure was recorded at 5-min intervals. Body temperature was measured at the right upper arm, precordium, abdomen, lumbus, right hallux, right femur, and right auditory canal.

Results

Skin temperature increased significantly at and near the hot pack application site, but this finding showed no relationship with body composition indices. The warmability distal to the application site was negatively correlated with the body water content index. The auditory canal temperature did not change in any of the sessions.

Conclusions

Hot pack usage alone did not increase the deep-body temperature and only increased the temperature around the application area. Moreover, higher body water content may allow for easier dissipation of heat from the peripheral extremities.
The radiological appearances of lung cancer treated with immunotherapy
Authors: Milanese Gianluca , Mazzaschi Giulia , Ledda Roberta Eufrasia , Balbi Maurizio , Lamorte Sveva , Caminiti Caterina , Colombi Davide , Tiseo Marcello , Silva Mario , Sverzellati Nicola
Published: November 2022

Therapy and prognosis of several solid and hematologic malignancies, including non-small cell lung cancer (NSCLC), have been favourably impacted by the introduction of immune checkpoint inhibitors (ICIs). Their mechanism of action relies on the principle that some cancers can evade immune surveillance by expressing surface inhibitor molecules, known as “immune checkpoints”. ICIs aim to conceal tumoural checkpoints on the cell surface and reinvigorate the ability of the host immune system to recognize tumour cells, triggering an antitumoural immune response.

In this review, we will focus on the imaging patterns of different responses occurring in patients treated by ICIs. We will also discuss imaging findings of immune-related adverse events (irAEs), along with current and future perspectives of metabolic imaging. Finally, we will explore the role of radiomics in the setting of ICI-treated patients.
Η θεραπεία πόνου κατά την Ιπποκρατική εποχή και οι ομοιότητες με την παραδοσιακή κινέζικη ιατρική
Authors: Τριανταφύλλου Χρήστος
Published: November 2022

Για την θεραπεία πόνου κατά την Ιπποκρατική εποχή και τις ομοιότητες με την παραδοσιακή Κινεζική Ιατρική , μελετήσαμε τις βασικές πηγές της Ιπποκρατικής Ιατρικής που διατίθενται στη βιβλιογραφία και συγκρίναμε τα δόγματα της με αυτά της Παραδοσιακής Κινέζικης Ιατρικής. Πολλές ομοιότητες φαίνεται να υπάρχουν μεταξύ αυτών των αρχαίων αλλά διακριτών ιατρικών σχολών και πολλές τοπογραφικά κοινές τοποθεσίες στην ονοματολογία του βελονισμού και στα σημεία παρέμβασης που πρότεινε ο Ιπποκράτης για τα περισσότερα από τα επώδυνα σύνδρομα. Η πιθανή αλληλεπίδρασης μεταξύ των φιλοξενούμενων πολιτισμών τους δεν μπορεί να αποδειχθεί, αλλά φαίνεται αρκετά πιθανή.
Pulmonary Arterial Hypertension Associated With Primary Sjögren’s Syndrome: An Unusual Association
Authors: Ribeirinho-Soares Pedro , Sousa Estela , Silva Inês , Almeida Jorge
Published: November 2022

Pulmonary arterial hypertension (PAH) is an increasingly recognised clinical entity that is associated with connective tissue disease (CTD) in up to one quarter of all diagnoses. Sjögren’s syndrome (SS) is a chronic autoimmune disease characterised by ocular and oral dryness resulting from lacrimal and salivary gland dysfunction. Additionally, SS may involve virtually any organ system and, as a result, the disease is characterised by pleomorphic clinical manifestations. However, SS-PAH reports are scarce, and the area remains insufficiently studied. We present a case of a 75-year-old female with a new diagnosis of PAH and SS.
Shaping inflammatory bowel disease care through patient choice and joint decision-making
Authors: Ludlow Helen
Published: November 2022

Patient choice and shared decision-making are priorities in the treatment of inflammatory bowel disease (IBD). The choices facilitated by UK IBD services were affected by the COVID-19 pandemic from when it began to affect the UK in early 2020, with an almost overnight stoppage of face-to-face outpatient clinic appointments and a recognition that some IBD treatments, such as steroids, may have a negative impact on patient outcome. Many IBD services struggled with staff redeployment and growing demand from unwell and concerned patients. Subsequent changes in treatment pathways resulted in some patients accessing biologic therapies at earlier stages than previously. The need to involve patients in decisions regarding their care became more important than ever, due to the potential impact of the pandemic on their disease and treatment pathway. This impact interacted with other key factors affecting patient choice, such as the perceived and real-world costs of biologic and biosimilar treatments. This review looks at how COVID-19 affected patient choice and how IBD clinical nurse specialists can use the principles of joint decision-making to move forward in developing IBD services for the future.