bluuebunny/crossref_metadata_2025 huggingface dataset:
Multiyear Typology of Long-Range Transported Aerosols over Europe
Authors: Nicolae Victor , Talianu Camelia , Andrei Simona , Antonescu Bogdan , Ene Dragoș , Nicolae Doina , Dandocsi Alexandru , Toader Victorin-Emilian , Ștefan Sabina , Savu Tom , Vasilescu Jeni
Published: August 2019

In this study, AERONET (Aerosol Robotic Network) and EARLINET (European Aerosol Research Lidar Network) data from 17 collocated lidar and sun photometer stations were used to characterize the optical properties of aerosol and their types for the 2008–2018 period in various regions of Europe. The analysis was done on six cluster domains defined using circulation types around each station and their common circulation features. As concluded from the lidar photometer measurements, the typical aerosol particles observed during 2008–2018 over Europe were medium-sized, medium absorbing particles with low spectral dependence. The highest mean values for the lidar ratio at 532 nm were recorded over Northeastern Europe and were associated with Smoke particles, while the lowest mean values for the Angstrom exponent were identified over the Southwest cluster and were associated with Dust and Marine particles. Smoke (37%) and Continental (25%) aerosol types were the predominant aerosol types in Europe, followed by Continental Polluted (17%), Dust (10%), and Marine/Cloud (10%) types. The seasonal variability was insignificant at the continental scale, showing a small increase in the percentage of Smoke during spring and a small increase of Dust during autumn. The aerosol optical depth (AOD) slightly decreased with time, while the Angstrom exponent oscillated between “hot and smoky” years (2011–2015) on the one hand and “dusty” years (2008–2010) and “wet” years (2017–2018) on the other hand. The high variability from year to year showed that aerosol transport in the troposphere became more and more important in the overall balance of the columnar aerosol load.
Response of Urban Heat Stress to Heat Waves in Athens (1960–2017)
Authors: Katavoutas George , Founda Dimitra
Published: August 2019

The increasing frequency, intensity and duration of heat waves seem to follow the observed global warming in recent decades. Vulnerability to heat waves is expected to increase in urban environments mainly due to population density and the effect of the urban heat island that make cities hotter than surrounding non-urban areas. The present study focuses on a vulnerable area of the eastern Mediterranean, already characterized as a ‘hot spot’ with respect to heat-related risk and investigates the change in heat stress levels during heat wave compared to non-heat wave conditions as well as the way that heat stress levels respond to heat waves in urban, compared to non-urban, environments. The adoption of a metric accounting for both the intensity and duration of the hot event yielded a total of 46 heat wave episodes over a nearly 60-year period, but with very rare occurrence until the late 1990s and a profound increased frequency thereafter. The results reveal a difference of at least one thermal stress category between heat wave and non-heat wave periods, which is apparent across the entire range of the thermal stress distribution. The analysis demonstrates a robust intensification of nighttime heat stress conditions in urban, compared to non-urban, sites during severe heat waves. Nevertheless, severe heat waves almost equalize heat stress conditions between urban and non-urban sites during midday.
Quantitative Analysis of Cerium-Gallium Alloys Using a Hand-Held Laser Induced Breakdown Spectroscopy Device
Authors: Rao Ashwin P. , Cook Matthew T. , Hall Howard L. , Shattan Michael B.
Published: August 2019

A hand-held laser-induced breakdown spectroscopy device was used to acquire spectral emission data from laser-induced plasmas created on the surface of cerium-gallium alloy samples with Ga concentrations ranging from 0–3 weight percent. Ionic and neutral emission lines of the two constituent elements were then extracted and used to generate calibration curves relating the emission line intensity ratios to the gallium concentration of the alloy. The Ga I 287.4-nm emission line was determined to be superior for the purposes of Ga detection and concentration determination. A limit of detection below 0.25% was achieved using a multivariate regression model of the Ga I 287.4-nm line ratio versus two separate Ce II emission lines. This LOD is considered a conservative estimation of the technique’s capability given the type of the calibration samples available and the low power (5 mJ per 1-ns pulse) and resolving power ( λ / Δ λ = 4000) of this hand-held device. Nonetheless, the utility of the technique is demonstrated via a detailed mapping analysis of the surface Ga distribution of a Ce-Ga sample, which reveals significant heterogeneity resulting from the sample production process.
Phenolic Content of Apple Juice for Cider Making as Influenced by Common Pre-Fermentation Processes Using Two Analytical Methods
Authors: Way Madeleine L. , Jones Joanna E. , Swarts Nigel D. , Dambergs Robert G.
Published: August 2019

Pre-fermentation methods can influence the cider produced from apple juice. This study analyses the influence of pre-fermentation methods; maceration and press fractioning, on the total phenolic content of juice from four apple varieties; ‘Red Delicious’, ‘Pink Lady’, ‘Sturmer’, and ‘Bulmer’s Norman’. The must was macerated for 0 or 2 h and juice was collected at free run or under 200 kpa. Base juice characteristics and total phenolic content was analysed using the Folin–Ciocalteu method and spectrophotometric absorbance at 280 nm (A280), a method used for the analysis of white wine. Both methods of analysing the total phenolic content were used, measuring the same samples to determine if the methods are comparable. No interaction was found between pre-fermentation technique and the results varied by variety and analytical method. High pressure improved the phenolic extraction for ‘Bulmer’s Norman’ juice compared to free run juice when analysed by A280. Non-macerated juice had higher total phenolic content than macerated juice for ‘Red Delicious’ and ‘Pink Lady’ juice when analysed using the Folin–Ciocalteu method. There was a moderate positive correlation between the analytical methods.
How the 26S Proteasome Degrades Ubiquitinated Proteins in the Cell
Authors: Coll-Martínez Bernat , Crosas Bernat
Published: August 2019

The 26S proteasome is the central element of proteostasis regulation in eukaryotic cells, it is required for the degradation of protein factors in multiple cellular pathways and it plays a fundamental role in cell stability. The main aspects of proteasome mediated protein degradation have been highly (but not totally) described during three decades of intense cellular, molecular, structural and chemical biology research and tool development. Contributions accumulated within this time lapse allow researchers today to go beyond classical partial views of the pathway, and start generating almost complete views of how the proteasome acts inside the cell. These views have been recently reinforced by cryo-electron microscopy and mechanistic works that provide from landscapes of proteasomal populations distributed in distinct intracellular contexts, to detailed shots of each step of the process of degradation of a given substrate, of the factors that regulate it, and precise measurements of the speed of degradation. Here, we present an updated digest of the most recent developments that significantly contribute in our understanding of how the 26S proteasome degrades hundreds of ubiquitinated substrates in multiple intracellular environments.
Colletotrichum Gloesporioides Inhibition In Situ by Chitosan-Ruta graveolens Essential Oil Coatings: Effect on Microbiological, Physicochemical, and Organoleptic Properties of Guava (Psidium guajava L.) during Room Temperature Storage
Authors: Grande Tovar Carlos David , Delgado-Ospina Johannes , Navia Porras Diana Paola , Peralta-Ruiz Yeimmy , Cordero Alexander Pérez , Castro Jorge Iván , Chaur Valencia Manuel Noé , Mina José Hermínsul , Chaves López Clemencia
Published: August 2019

Guava is a fruit appreciated worldwide for its high content of bioactive compounds. However, it is considered a highly perishable fruit, generally attacked by pathogenic species such as the fungi Colletotrichum gloeosporioides, which causes anthracnosis. To diminish the losses caused by pathogenic fungi, coatings of chitosan (CS) with Ruta graveolens essential oil (RGEO) in different concentrations (0.5, 1.0, 1.5% v/v) were applied in situ and their effects on the physical properties and microbiological quality of the guavas were studied. The CS+RGEO coated fruits exhibited better physicochemical behavior and lower microbiological decay as compared to the uncoated guavas, demonstrating the effectiveness of the coatings, especially those with 1.5% of RGEO content. All the fruits coated had greater acceptance and quality than the controls, being more those with essential oil incorporation. In situ investigation of C. gloesporioides infection of guavas demonstrated that the CS+RGEO coated guavas showed a high percentage of inhibition in the development of anthracnose lesions. In the present investigation, an alternative method has been proposed to extend the stability of the guavas fruit up to 12 days with application in the food industry.
Endogenous Neuropeptide Nocistatin Is a Direct Agonist of Acid-Sensing Ion Channels (ASIC1, ASIC2 and ASIC3)
Authors: Osmakov Dmitry I. , Koshelev Sergey G. , Ivanov Igor A. , Andreev Yaroslav A. , Kozlov Sergey A.
Published: August 2019

Acid-sensing ion channel (ASIC) channels belong to the family of ligand-gated ion channels known as acid-sensing (proton-gated) ion channels. Only a few activators of ASICs are known. These are exogenous and endogenous molecules that cause a persistent, slowly desensitized current, different from an acid-induced current. Here we describe a novel endogenous agonist of ASICs—peptide nocistatin produced by neuronal cells and neutrophils as a part of prepronociceptin precursor protein. The rat nocistatin evoked currents in X. laevis oocytes expressing rat ASIC1a, ASIC1b, ASIC2a, and ASIC3 that were very similar in kinetic parameters to the proton-gated response. Detailed characterization of nocistatin action on rASIC1a revealed a proton-like dose-dependence of activation, which was accompanied by a dose-dependent decrease in the sensitivity of the channel to the protons. The toxin mambalgin-2, antagonist of ASIC1a, inhibited nocistatin-induced current, therefore the close similarity of mechanisms for ASIC1a activation by peptide and protons could be suggested. Thus, nocistatin is the first endogenous direct agonist of ASICs. This data could give a key to understanding ASICs activation regulation in the nervous system and also could be used to develop new drugs to treat pathological processes associated with ASICs activation, such as neurodegeneration, inflammation, and pain.
For Better or Worse: The Potential for Dose Limiting the On-Target Toxicity of PI 3-Kinase Inhibitors
Authors: Buchanan Christina , Lee Kate , Shepherd Peter
Published: August 2019

The hyper-activation of the phosphoinositide (PI) 3-kinase signaling pathway is a hallmark of many cancers and overgrowth syndromes, and as a result, there has been intense interest in the development of drugs that target the various isoforms of PI 3-kinase. Given the key role PI 3-kinases play in many normal cell functions, there is significant potential for the disruption of essential cellular functions by PI 3-kinase inhibitors in normal tissues; so-called on-target drug toxicity. It is, therefore, no surprise that progress within the clinical development of PI 3-kinase inhibitors as single-agent anti-cancer therapies has been slowed by the difficulty of identifying a therapeutic window. The aim of this review is to place the cellular, tissue and whole-body effects of PI 3-kinase inhibition in the context of understanding the potential for dose limiting on-target toxicities and to introduce possible strategies to overcome these.
Overexposure to Bisphenol A and Its Chlorinated Derivatives of Patients with End-Stage Renal Disease during Online Hemodiafiltration
Authors: Bacle Astrid , Dupuis Antoine , Belmouaz Mohamed , Bauwens Marc , Cambien Guillaume , Venisse Nicolas , Pierre-Eugene Pascale , Potin Sophie , Migeot Virginie , Ayraud-Thevenot Sarah
Published: August 2019

The health safety conditions governing the practice of online hemodiafiltration (OL-HDF) do not yet incorporate the risks related to the presence of endocrine disruptors such as bisphenol A (BPA). The aim of this study was to assess, for the first time, the exposure to BPA but also to its chlorinated derivatives (ClxBPA) (100 times more estrogenic than BPA) during OL-HDF. We demonstrated that BPA is transmitted by the different medical devices used in OL-HDF: ultrafilters, dialysis concentrate cartridges (and not only dialyzers, as previously described). Moreover, BPA has been found in dialysis water as well as in ultrapure dialysate and replacement fluid due to contamination of water coming from municipal network. Indeed, due to contaminations provided by both ultrafilters and water, high levels of BPA were determined in the infused replacement fluid (1033 ng.L−1) from the beginning of the session. Thus, our results demonstrate that dialysis water must be considered as an important exposure source to endocrine disruptors, especially since other micropollutants such as ClxBPA have also been detected in dialysis fluids. While assessment of the impact of this exposure remains to be done, these new findings should be taken into account to assess exposure risks in end-stage renal disease patients.
MTHFR Gene Mutations Correlate with White Matter Disease Burden and Predict Cerebrovascular Disease and Dementia
Authors: Cajavilca Christian E. , Gadhia Rajan R. , Román Gustavo C.
Published: August 2019

The incidence of dementia is on the rise and expected to continue to increase in the foreseeable future. Two of the most common subtypes of dementia are Alzheimer’s subtype and vascular dementia. Hyperhomocysteinemia has been shown to serve as a risk factor for dementia due to an associated blood–brain barrier dysfunction and subsequent small-vessel disease pathology. There are varying causes for hyperhomocysteinemia, including genetic and dietary, among others. We highlight the importance of identifying hyperhomocysteinemia as a potential etiologic and therapeutic target for the most common subtypes of dementia.
Classification of Alzheimer’s Disease with and without Imagery Using Gradient Boosted Machines and ResNet-50
Authors: Fulton Lawrence , Dolezel Diane , Harrop Jordan , Yan Yan , Fulton Christopher
Published: August 2019

Background. Alzheimer’s is a disease for which there is no cure. Diagnosing Alzheimer’s disease (AD) early facilitates family planning and cost control. The purpose of this study is to predict the presence of AD using socio-demographic, clinical, and magnetic resonance imaging (MRI) data. Early detection of AD enables family planning and may reduce costs by delaying long-term care. Accurate, non-imagery methods also reduce patient costs. The Open Access Series of Imaging Studies (OASIS-1) cross-sectional MRI data were analyzed. A gradient boosted machine (GBM) predicted the presence of AD as a function of gender, age, education, socioeconomic status (SES), and a mini-mental state exam (MMSE). A residual network with 50 layers (ResNet-50) predicted the clinical dementia rating (CDR) presence and severity from MRI’s (multi-class classification). The GBM achieved a mean 91.3% prediction accuracy (10-fold stratified cross validation) for dichotomous CDR using socio-demographic and MMSE variables. MMSE was the most important feature. ResNet-50 using image generation techniques based on an 80% training set resulted in 98.99% three class prediction accuracy on 4139 images (20% validation set) at Epoch 133 and nearly perfect multi-class predication accuracy on the training set (99.34%). Machine learning methods classify AD with high accuracy. GBM models may help provide initial detection based on non-imagery analysis, while ResNet-50 network models might help identify AD patients automatically prior to provider review.
Cerebral Small Vessel Disease Associated with Subclinical Vascular Damage Indicators in Asymptomatic Hypertensive Patients
Authors: Hernández-Díaz Zenaida Milagros , Peña-Sánchez Marisol , González-Quevedo Alina , González-García Sergio , Arias-Cadena Paula Andrea , Brown-Martínez Marta , Betancourt-Loza Mélany , Cordero-Eiriz Anay
Published: August 2019

Background: Cerebral small vessel disease (CSVD) is frequent in patients with cardiovascular risk factors including arterial hypertension, and it is associated with vascular damage in other organs and the risk of stroke, cognitive impairment, and dementia. Early diagnosis of CSVD could prevent deleterious consequences. Objective: To characterize CSVD associated with indicators of subclinical vascular damage in asymptomatic hypertensive patients. Materials and Methods: Participants were hypertensive (HT) and non-hypertensive (non-HT) individuals; without signs of cerebrovascular disease, dementia, and chronic renal failure. For CSVD, white matter hyperintensities (WMH), enlarged Virchow–Robin perivascular spaces (EVRPS), lacunar infarcts, and microbleeds were investigated. Subclinical vascular damage was evaluated (hypertensive retinopathy, microalbuminuria, and extracranial carotid morphology: intima media thickness (IMT) and atheroma plaque). Results: CSVD MRI findings were more frequent in HT; as well as greater intimal thickening. The IMT and/or plaque was significantly associated with all MRI variables; but retinopathy was correlated with EVRPS and lacunar infarcts. Only microalbuminuria was related to the greater severity of WMH in HT. Multivariate analysis evidenced that CSVD was independently associated with the combination of indicators of vascular damage and systolic blood pressure. Conclusions: Combining indicators of subclinical vascular damage, such as carotid morphological variables, microalbuminuria, and hypertensive retinopathy for early detection of CSVD in asymptomatic hypertensive patients could prove to be useful to take actions for the prevention of irreversible brain damage, which could lead to cognitive impairment, dementia and stroke.
Insights into the Electrochemical Behavior of Mercury on Graphene/SiC Electrodes
Authors: Shtepliuk Ivan , Vagin Mikhail , Yakimova Rositsa
Published: August 2019

Fast and real time detection of Mercury (Hg) in aqueous solutions is a great challenge due to its bio-accumulative character and the detrimental effect on human health of this toxic element. Therefore, development of reliable sensing platforms is highly desirable. Current research is aiming at deep understanding of the electrochemical response of epitaxial graphene to Mercury exposure. By performing cyclic voltammetry and chronoamperometry measurements as well as density functional theory calculations, we elucidate the nature of Hg-involved oxidation-reduction reactions at the graphene electrode and shed light on the early stages of Hg electrodeposition. The obtained critical information of Hg behavior will be helpful for the design and processing of novel graphene-based sensors.
Key Role of Obesity in Genitourinary Tumors with Emphasis on Urothelial and Prostate Cancers
Authors: Santoni Matteo , Cimadamore Alessia , Massari Francesco , Piva Francesco , Aurilio Gaetano , Martignetti Angelo , Scarpelli Marina , Di Nunno Vincenzo , Gatto Lidia , Battelli Nicola , Cheng Liang , Lopez-Beltran Antonio , Montironi Rodolfo
Published: August 2019

Background: In human populations, a certain amount of data correlate obesity/body mass index (BMI) with urothelial cancer (UC) and prostate cancer (PCa) occurrence, however this is not fully elucidated at all stages of disease. In an attempt to shed light on uncertain areas in such field, in the present review we illustrate the main molecular mechanisms linking obesity and cancer, focusing on the correlation between obesity and tumor risk, disease progression and response to chemo- and immunotherapy in patients with UC and the predictive/prognostic role of obesity in PCa patients treated with the currently available therapeutic approaches. Methods: We did a large-scale literature search on existing scientific websites focusing on keywords “obesity”, “body mass index (BMI)”, “urothelial cancer”, “prostate cancer”, “docetaxel”, “cabazitaxel”, “abiraterone acetate”, “enzalutamide”, and “radium223”. Results: Many adipocytes-induced molecules support tumor proliferation through activation of various cellular pathways. The available evidence in the postoperative setting do the role of BMI in oncological outcomes prediction still not completely clear. Likewise, in metastatic UC patients controversial results link the role of obesity/BMI with clinical outcomes of tumor response to chemotherapy. Adipose stromal cells recruitment, induced by PCa cells, from white adipose tissue to the tumor sites inducing cell invasiveness was associated with poor survival. Conflicting data, although more oriented towards a better survival outcome, resulted in obese patients treated with docetaxel. In PCa cell-lines a certain cabazitaxel chemo resistance adipose stromal cells (ASC)-mediated was demonstrated. In metastatic castration-resistant PCa patients with high BMI (>25 kg/m2) receiving abiraterone acetate there were significant worse survival outcomes, while in enzalutamide patients BMI did not affect survival outcome. In radium 223 patients higher BMI significantly correlated with favorable overall survival. Conclusions: The main focus of this review was to understand the interplay between obesity/BMI and UC/PCa. Several pathogenic cellular pathways exploring the issue are discussed, opening the way to challenging tailored treatments on the basis of BMI. Improving the knowledge of molecular connections between obesity and UC and PCa could favor the development of new therapies likely reducing chemo- and immunotherapy drug resistance.
Predictive Factors of Recurrence in Patients with Differentiated Thyroid Carcinoma: A Retrospective Analysis on 579 Patients
Authors: Medas Fabio , Canu Gian Luigi , Boi Francesco , Lai Maria Letizia , Erdas Enrico , Calò Pietro Giorgio
Published: August 2019

Differentiated thyroid carcinoma (DTC) is usually associated with a favorable prognosis. Nevertheless, up to 30% of patients present a local or distant recurrence. The aim of this study was to assess the incidence of recurrence after surgery for DTC and to identify predictive factors of recurrence. We included in this retrospective study 579 consecutive patients who underwent thyroidectomy for DTC from 2011 to 2016 at our institution. We observed biochemical or structural recurrent disease in 36 (6.2%) patients; five-year disease-free survival was 94.1%. On univariate analysis, male sex, histotype, lymph node yield, lymph node metastasis, extrathyroidal invasion and multicentricity were associated with significantly higher risk of recurrence, while microcarcinoma was correlated with significantly lower risk of recurrence. On multivariate analysis, only lymph node metastases (OR 4.724, p = 0.012) and microcarcinoma (OR 0.328, p = 0.034) were detected as independent predictive factors of recurrence. Postoperative management should be individualized and commensurate with the risk of recurrence: Patients with high-risk carcinoma should undergo strict follow-up and aggressive treatment. Furthermore, assessment of the risk should be repeated over time, considering individual response to therapy.
Synergy of NUP98-HOXA10 Fusion Gene and NrasG12D Mutation Preserves the Stemness of Hematopoietic Stem Cells on Culture Condition
Authors: Dong Yong , Xia Chengxiang , Weng Qitong , Wang Tongjie , Hu Fangxiao , Wang Kaitao , Liu Xiaofei , Geng Yang , Liu Lijuan , Wu Hongling , Du Juan
Published: August 2019

Natural hematopoietic stem cells (HSC) are susceptible and tend to lose stemness, differentiate, or die on culture condition in vitro, which adds technical challenge for maintaining bona fide HSC-like cells, if ever generated, in protocol screening from pluripotent stem cells. It remains largely unknown whether gene-editing of endogenous genes can genetically empower HSC to endure the culture stress and preserve stemness. In this study, we revealed that both NUP98-HOXA10HD fusion and endogenous Nras mutation modifications (NrasG12D) promoted the engraftment competitiveness of HSC. Furthermore, the synergy of these two genetic modifications endowed HSC with super competitiveness in vivo. Strikingly, single NAV-HSC successfully maintained its stemness and showed robust multi-lineage engraftments after undergoing the in vitro culture. Mechanistically, NUP98-HOXA10HD fusion and NrasG12D mutation distinctly altered multiple pathways involving the cell cycle, cell division, and DNA replication, and distinctly regulated stemness-related genes including Hoxa9, Prdm16, Hoxb4, Trim27, and Smarcc1 in the context of HSC. Thus, we develop a super-sensitive transgenic model reporting the existence of HSC at the single cell level on culture condition, which could be beneficial for protocol screening of bona fide HSC regeneration from pluripotent stem cells in vitro.
Early Diagnosis of Pathogen Infection by Cell-Based Activation Immunoassay
Authors: Bar-Haim Erez , Rotem Shahar , Elia Uri , Bercovich-Kinori Adi , Israeli Ma’ayan , Cohen-Gihon Inbar , Israeli Ofir , Erez Noam , Achdout Hagit , Zauberman Ayelet , Aftalion Moshe , Mamroud Emanuelle , Chitlaru Theodor , Cohen Ofer
Published: August 2019

Diagnostic identification of pathogens is usually accomplished by isolation of the pathogen or its substances, and should correlate with the time and site of infection. Alternatively, immunoassays such as enzyme-linked immunosorbent assay (ELISA) tests for quantification of serum antibodies are expedient and are usually employed for retrospective diagnostic of a particular infective agent. Here, the potential of cell-based immunoassays for early pathogen detection was evaluated by quantification of specific, antigen-activated, low-frequency IFNγ-secreting cells in mouse spleens following infection with various pathogens. Using enzyme-linked immunospot (ELISPOT) assays, specific responses were observed within 3–6 days following infection with F. tularensis, B. anthracis, Y. pestis, or Influenza virus. Blood samples collected from F. tularensis-infected mice revealed the presence of IFNγ-producing activated cells within one week post infection. When non-human primates were infected with B. anthracis, cellular response was observed in peripheral blood samples as early as five days post infection, 3–5 days earlier than serum antibodies. Finally, the expression pattern of genes in splenocytes of F. tularensis-infected mice was inspected by a transcriptomic approach, enabling the identification of potential host targets for the future development of genetic-based cellular immunoassays. Altogether, the data demonstrate the potential of cell-based immunoassays for early pathogen detection.
Ferlin Overview: From Membrane to Cancer Biology
Authors: Peulen Olivier , Rademaker Gilles , Anania Sandy , Turtoi Andrei , Bellahcène Akeila , Castronovo Vincent
Published: August 2019

In mammal myocytes, endothelial cells and inner ear cells, ferlins are proteins involved in membrane processes such as fusion, recycling, endo- and exocytosis. They harbour several C2 domains allowing their interaction with phospholipids. The expression of several Ferlin genes was described as altered in several tumoural tissues. Intriguingly, beyond a simple alteration, myoferlin, otoferlin and Fer1L4 expressions were negatively correlated with patient survival in some cancer types. Therefore, it can be assumed that membrane biology is of extreme importance for cell survival and signalling, making Ferlin proteins core machinery indispensable for cancer cell adaptation to hostile environments. The evidences suggest that myoferlin, when overexpressed, enhances cancer cell proliferation, migration and metabolism by affecting various aspects of membrane biology. Targeting myoferlin using pharmacological compounds, gene transfer technology, or interfering RNA is now considered as an emerging therapeutic strategy.
Microstructure Evolution and Failure Behavior of Stellite 6 Coating on Steel after Long-Time Service
Authors: Xiong Jiankun , Nie Fuheng , Zhao Haiyan , Zheng Liangliang , Luo Jun , Yang Lin , Wen Zhongbo
Published: August 2019

The microstructure evolution, elements diffusion and fracture behavior of the Stellite 6 weld overlay, deposited on 10Cr9Mo1VNbN (F91) steel by the tungsten inert gas (TIG) cladding process, were investigated after long-time service. Obvious diffusion of Fe occurred from the steel and fusion zone to the Stellite overlay, resulting in the microstructure evolution and hardness increase in the coating, where hard Co–Fe phases, σ phases (Fe–Cr metallic compounds) and Cr-rich carbides (Cr18.93Fe4.07C6) were formed. Besides, the width of the light zone, combined with the fusion zone and diffusion zone, increased significantly to a maximum value of 2.5 mm. The fracture of the Stellite coating samples mainly occurred in the light zone, which was caused by the formation and growth of circumferential crack and radial crack under high temperature and pressure conditions. Moreover, the micro-hardness values in the light zone increased to the maximum (470–680 HV) due to the formation and growth of brittle Co–Fe phases. The formation of these cracks might be caused by formed brittle phases and changes of micro-hardness during service.
Photostabilizing Efficiency of Acrylic-based Bamboo Exterior Coatings Combining Benzotriazole and Zinc Oxide Nanoparticles
Authors: Rao Fei , Zhang Yahui , Bao Minzhen , Zhang Zhiyuan , Bao Yongjie , Li Neng , Chen Yuhe , Yu Wenji
Published: August 2019

Long-term exposure to ultraviolet (UV) light can degrade and discolor bamboo; thus, coatings to protect it from UV exposure are required, especially for outdoor use. Benzotriazole (BTZ) and zinc oxide nanoparticles (NZnO) are organic and inorganic UV absorbers commonly used in UV shielding coatings. This study investigated the photostabilizing efficiency of acrylic-based bamboo exterior coatings using a combination of BTZ and NZnO. Different film formulations covering bamboo substrates were irradiated with artificial UV light for 500 h to accelerate aging. The UV-shielding effect on bamboo beneath various films was determined by CIELAB color space and Fourier transform infrared (FTIR) analysis. The film effectiveness was analyzed by scanning electron microscopy, wettability, UV-vis spectroscopy, and FTIR-attenuated total reflection (ATR) spectroscopy. Films containing BTZ provided higher resistance to photodegradation and more effectively inhibited photodiscoloration of the bamboo substrates than those prepared solely with NZnO. After 500 h of UV irradiation, the BTZ–NZnO film containing 2 wt % BTZ and 1 wt % NZnO showed the best coating performance. Strong synergistic effects were detected in the BTZ–NZnO coatings, particularly for the 2:1 ratio formulation. This study also demonstrated the potential of combining BTZ and NZnO as additives for developing stable, effective UV-shielding bamboo exterior coatings for outdoor applications.
Effects of Cobalt Content on the Microstructure, Mechanical Properties and Cavitation Erosion Resistance of HVOF Sprayed Coatings
Authors: Liu Ji , Bai Xiuqin , Chen Tongzhou , Yuan Chengqing
Published: August 2019

Cobalt-based alloy coatings and WC-Co-based ceramic–metal (cermet) coatings have been widely used because of their desirable mechanical properties and corrosion resistance. In this work, the influence of Co content on the microstructure, mechanical properties and cavitation erosion (CE) resistance were investigated. A cobalt-based alloy coating, a WC-12Co coating, and a WC-17Co cermet coating were deposited by high-velocity oxygen fuel (HVOF) spraying on 1Cr18Ni9Ti substrates. Results indicate that the cobalt-based alloy coating had the largest surface roughness because surface-bonded particles of lower plastic deformation were flattened. The existence of WC particles had led to an increase in hardness and improved the fracture toughness due to inhibit crack propagation. The pore appeared at the interface between WC particles, and the matrix phase had introduced an increase in porosity. With the increase in Co content, the cohesion between matrix friction and WC particles increased and then decreased the porosity (from 0.99% to 0.84%) and surface roughness (Ra from 4.49 to 2.47 μm). It can be concluded that the hardness had decreased (from 1181 to 1120 HV0.3) with a decrease in WC hard phase content. On the contrary, the fracture toughness increased (from 4.57 to 4.64 MPa∙m1/2) due to higher energy absorption in the matrix phase. The WC-12Co and WC-17Co coatings with higher hardness and fracture toughness exhibited better CE resistance than the cobalt-based alloy coating, increasing more than 20% and 16%, respectively. Especially, the WC-12Co coating possessed the best CE resistance and is expected to be applicable in the hydraulic machineries.
Irradiance in Mixed Coherent/Incoherent Structures: An Analytical Approach
Authors: Puhan Janez , Bűrmen Árpád , Tuma Tadej , Fajfar Iztok
Published: August 2019

We propose a new method for a light energy flux density (or irradiance) calculation in an arbitrary multilayer stack containing coherent and incoherent layers. Although the well known General Transfer-Matrix Method (GTMM) can be successfully used for the overall reflectance and transmittance calculation, it does not allow us to obtain the corresponding irradiance depth profile straightforwardly. We show in this paper that subsequent phase-shift integrations over the incoherent layers result in the reflectance and transmittance expressions identical to those of the GTMM formulation. However, the alternative mathematical approach allows us to derive an analytical expression for irradiance at an arbitrary depth of the multilayer stack, thus making it possible to calculate the absorptance depth profile. In fact, the GTMM expressions for the overall reflectance and transmittance turn out to be special cases of the irradiance calculation at the incident and emergent surface of the multilayer stack. Consequently, the proposed Phase-shift Integration Method (PIM) represents a continuous irradiance calculation model without any energy imbalances on layer interfaces. In addition, since we are able to obtain analytical layer thickness derivatives, the PIM is suitable for use with gradient optimization methods. We verify the method on three cases of an encapsulated bifacial heterojunction silicon (HJ Si) solar cell, a perovskite solar cell, and a perovskite/silicon tandem solar cell, which all consist of thin and thick layers.
Bio-Inspired Deep-CNN Pipeline for Skin Cancer Early Diagnosis
Authors: Rundo Francesco , Banna Giuseppe Luigi , Conoci Sabrina
Published: August 2019

Skin cancer is the most common type of cancer, as also among the riskiest in the medical oncology field. Skin cancer is more common in people who work or practice outdoor sports and those that expose themselves to the sun. It may also develop years after radiographic therapy or exposure to substances that cause cancer (e.g., arsenic ingestion). Numerous tumors can affect the skin, which is the largest organ in our body and is made up of three layers: the epidermis (superficial layer), the dermis (middle layer) and the subcutaneous tissue (deep layer). The epidermis is formed by different types of cells: melanocytes, which have the task of producing melanin (a pigment that protects against the damaging effects of sunlight), and the more numerous keratinocytes. The keratinocytes of the deepest layer are called basal cells and can give rise to basal cell carcinomas. We are interested in types of skin cancer that originate from melanocytes, i.e., the so-called melanomas, because it is the most aggressive. The dermatologist, during a complete visit, evaluates the personal and family history of the patient and carries out an accurate visual examination of the skin, thanks to the use of epi-luminescence (or dermoscopy), a special technique for enlarging and illuminating the skin. This paper mentions one of the most widely used diagnostic methods due to its simplicity and validity—the ABCDE method (Asymmetry, edge irregularity, Color Variegation, Diameter, Evolution). This methodology, based on “visual” investigation by the dermatologist and/or oncologist, has the advantage of not being invasive and quite easy to perform. This approach is affected by the opinion of who (physicians) applies it. For this reason, certain diagnosis of cancer is made, however, only with a biopsy, a procedure during which a portion of tissue is taken and then analyzed under a microscope. Obviously, this is particularly invasive for the patient. The authors of this article have analyzed the development of a method that obtains with good accuracy the early diagnosis of skin neoplasms using non-invasive, but at the same time, robust methodologies. To this end, the authors propose the adoption of a deep learning pipeline based on morphological analysis of the skin lesion. The results obtained and compared with previous approaches confirm the good performance of the proposed pipeline.
Research and Implementation of ε-SVR Training Method Based on FPGA
Authors: Wu Ruidong , Liu Bing , Fu Jiafeng , Xu Mingzhu , Fu Ping , Li Junbao
Published: August 2019

Online training of Support Vector Regression (SVR) in the field of machine learning is a computationally complex algorithm. Due to the need for multiple iterative processing in training, SVR training is usually implemented on computer, and the existing training methods cannot be directly implemented on Field-Programmable Gate Array (FPGA), which restricts the application range. This paper reconstructs the training framework and implementation without precision loss to reduce the total latency required for matrix update, reducing time consumption by 90%. A general ε-SVR training system with low latency is implemented on Zynq platform. Taking the regression of samples in two-dimensional as an example, the maximum acceleration ratio is 27.014× compared with microcontroller platform and the energy consumption is 12.449% of microcontroller. From the experiments for the University of California, Riverside (UCR) time series data set. The regression results obtain excellent regression effects. The minimum coefficient of determination is 0.996, and running time is less than 30 ms, which can meet the requirements of different applications for real-time regression.
Switch Elements with S-Shaped Current-Voltage Characteristic in Models of Neural Oscillators
Authors: Boriskov Petr , Velichko Andrei
Published: August 2019

In this paper, we present circuit solutions based on a switch element with the S-type I–V characteristic implemented using the classic FitzHugh–Nagumo and FitzHugh–Rinzel models. Using the proposed simplified electrical circuits allows the modeling of the integrate-and-fire neuron and burst oscillation modes with the emulation of the mammalian cold receptor patterns. The circuits were studied using the experimental I–V characteristic of an NbO2 switch with a stable section of negative differential resistance (NDR) and a VO2 switch with an unstable NDR, considering the temperature dependences of the threshold characteristics. The results are relevant for modern neuroelectronics and have practical significance for the introduction of the neurodynamic models in circuit design and the brain–machine interface. The proposed systems of differential equations with the piecewise linear approximation of the S-type I–V characteristic may be of scientific interest for further analytical and numerical research and development of neural networks with artificial intelligence.
Magnetic Resonance Imaging of Methane Hydrate Formation and Dissociation in Sandstone with Dual Water Saturation
Authors: Almenningen Stian , Fotland Per , Ersland Geir
Published: August 2019

This paper reports formation and dissociation patterns of methane hydrate in sandstone. Magnetic resonance imaging spatially resolved hydrate growth patterns and liberation of water during dissociation. A stacked core set-up using Bentheim sandstone with dual water saturation was designed to investigate the effect of initial water saturation on hydrate phase transitions. The growth of methane hydrate (P = 8.3 MPa, T = 1–3 °C) was more prominent in high water saturation regions and resulted in a heterogeneous hydrate saturation controlled by the initial water distribution. The change in transverse relaxation time constant, T2, was spatially mapped during growth and showed different response depending on the initial water saturation. T2 decreased significantly during growth in high water saturation regions and remained unchanged during growth in low water saturation regions. Pressure depletion from one end of the core induced a hydrate dissociation front starting at the depletion side and moving through the core as production continued. The final saturation of water after hydrate dissociation was more uniform than the initial water saturation, demonstrating the significant redistribution of water that will take place during methane gas production from a hydrate reservoir.
Modified Rotor Flux Estimators for Stator-Fault-Tolerant Vector Controlled Induction Motor Drives
Authors: Dybkowski Mateusz , Bednarz Szymon Antoni
Published: August 2019

This paper deals with fault-tolerant control (FTC) of an induction motor (IM) drive. An inter-turn short circuit (ITSC) of the stator windings was taken into consideration, which is one of the most common internal faults of induction machines. The sensitivity of the classic, well-known voltage and current models to the stator winding faults was analyzed. It has been shown that these classical state variable estimators are sensitive to induction motor parameter changes during stator winding failure, which results in unstable operation of the direct field-oriented control (DFOC) drive. From a safety-critical applications point of view, it is vital to guarantee stable operation of the drive even during faults of the machine. Therefore, a new FTC system has been proposed, which consists of new modified rotor flux estimators, robust to stator winding faults. A detailed description of the proposed system is presented herein, as well as the results of simulation and experimental tests. Simulation analyses were performed using MATLAB/Simulink software. Experimental tests were carried out on the experimental test bench with a dSpace DS1103 card. The proposed solution could be applied as an alternative rotor flux estimation technique for the modern FTC drive.
Power Control Method for Energy Efficient Buffer-Aided Relay Systems
Authors: Joung Jingon , Lee Han Lim , Zhao Jian , Kang Xin
Published: August 2019

In this paper, a power control method is proposed for a buffer-aided relay node (RN) to enhance the energy efficiency of the RN system. By virtue of a buffer, the RN can reserve the data at the buffer when the the channel gain between an RN and a destination node (DN) is weaker than that between SN and RN. The RN then opportunistically forward the reserved data in the buffer according to channel condition between the RN and the DN. By exploiting the buffer, RN reduces transmit power when it reduces the transmit data rate and reserve the data in the buffer. Therefore, without any total throughput reduction, the power consumption of RN can be reduced, resulting in the energy efficiency (EE) improvement of the RN system. Furthermore, for the power control, we devise a simple power control method based on a two-dimensional surface fitting model of an optimal transmit power of RN. The proposed RN power control method is readily and locally implementable at the RN, and it can significantly improve EE of the RN compared to the fixed power control method and the spectral efficiency based method as verified by the rigorous numerical results.
Exploitation of Mowed Grass from Green Areas by Means of Anaerobic Digestion: Effects of Grass Conservation Methods (Drying and Ensiling) on Biogas and Biomethane Yield
Authors: Chiumenti Alessandro , Pezzuolo Andrea , Boscaro Davide , da Borso Francesco
Published: August 2019

Grass from landscape management or from agricultural practices is currently destined mainly for composting, with the production of a valuable product; however, this process demands energy. Anaerobic digestion, instead, represents an energy-positive process that results in the production of fuel, biogas, and a fertilizer, namely digestate. Previous tests for the evaluation of biogas yield from freshly harvested grass gave promising results. However, for a practical exploitation of this resource, appropriate conservation is necessary in order to enable the daily load of digesters while reducing the loss of organic matter. The present work is focused on the evaluation of biogas and methane yield from dried and ensiled grass (without conditioning) in order to assess eventual biogas potential losses in comparison to digested fresh grass. Tests were performed with grass collected from riverbanks (Veneto, Northern Italy) in batch, lab scale digesters. Dry and ensiled grass showed a good potential for exploitation in the anaerobic digestion process, reaching biogas yields of 565.9 and 573.4 NL∙kgVS−1, respectively. Compared to the biogas yield of 639.7 NL∙kgVS−1 of the fresh grass, the conservation treatment determined yield reductions of 11.5% and 10.4% for dried and ensiled grass, respectively. However, considering the methane yields, conservation treatments showed lower reductions, amounting to 4.8% for dry grass and 0.5% for ensiled grass; presumably the higher concentration of organic acids in ensiled grass determined a higher methane content in biogas and the consequently lower reduction of methane yield.
Ophelimus migdanorum Molina-Mercader sp. nov. (Hymenoptera: Eulophidae): Application of Integrative Taxonomy for Disentangling a Polyphenism Case in Eucalyptus globulus Labill Forest in Chile
Authors: Molina-Mercader Gloria , Angulo Andrés , Olivares Tania , Sanfuentes Eugenio , Castillo-Salazar Miguel , Rojas Eladio , Toro-Núñez Oscar , Benítez Hugo , Hasbún Rodrigo
Published: August 2019

In 2003, a new gall-inducing wasp of the genus Ophelimus was detected in the Valparaíso Region (Chile), affecting tree plantations of Eucalyptus globulus Labill and Eucalyptus camaldulensis Dehnh. Since then Ophelimus has been frequently detected in different plantations in Chile, covering a widespread area. A preliminary collaborative study suggests that the micro-wasp detected should be classified as a new Ophelimus species. In this paper, using an integrative approach (including genetic, morphological, and behavioral data), we addressed the delimitation and description of this new species. This study involved the use of brood adult specimens, raised at the laboratory of MIPlagas Ltda., from infested twigs of E. globulus collected in several localities between of Valparaíso and Los Lagos Regions (Chile). Morphological structures were described according to current Eulophidae taxonomic keys, as well as additional traits, such as gall morphology and behavior. Genetic characterization was implemented using a phylogenetic approach, based on a 648 bp specific fragment of the mitochondrial Cytochrome Oxidase I gene (COI 5 region) obtained from collected specimens and available databases (Genbank, NCBI, and BOLDSystem). Specifically, distinctive patterns of variation were detected in traits like gall and antennae morphology, growth habit trends, and a notorious polyphenism in the setae from the sub marginal vein. Overall evidence suggests that this new entity should be considered a new species in Ophelimus, which is henceforth named Ophelimus migdanorum Molina-Mercader.