bluuebunny/crossref_metadata_2025 huggingface dataset:
Long-term prevalence follow-up (1967–2022) of HTLV-2 among vulnerable indigenous populations in the Amazon region of Brazil
Authors: Ishak Ricardo , de Oliveira Guimarães Ishak Marluísa , Abreu Isabella Nogueira , Machado Luiz Fernando Almeida , Lima Sandra Souza , Queiroz Maria Alice Freitas , Cayres-Vallinoto Izaura Maria , Guerreiro João Farias , Vallinoto Antonio Carlos Rosário
Published: June 2023

Introduction

Human T-lymphotropic virus 2 (HTLV-2) has been described for more than 30 years as an endemic infection in Brazilian indigenous populations, with its occurrence varying by age and sex, maintained mainly by sexual intercourse and mother-to-child transmission, favoring intrafamilial aggregation.

Methods

The epidemiological scenario of HTLV-2 infection has been described among communities of the Amazon region of Brazil (ARB), with the number of retrospective positive blood samples increasing for more than 50 years.

Results

Five publications were selected that showed the presence of HTLV-2 in 24 of 41 communities; the prevalence of infection was described among 5,429 individuals at five points in time. Among the Kayapó villages, the prevalence rates were described according to age and sex and reached up to 41.2%. Three communities (Asurini, Araweté, and Kaapor) were kept virus free for 27 to 38 years of surveillance. Low, medium and high prevalence levels of infection were defined, and two pockets of high endemicity were shown in the state of Pará, pointing to the Kikretum and Kubenkokrê Kayapó villages as the epicenter of HTLV-2 in the ARB.

Discussion

The prevalence rates over the years have shown a decline among the Kayapó (from 37.8 to 18.4%) and an apparent change to a higher prevalence among females, but not during the first decade of life, usually associated with transmission from mother to child. Sociocultural and behavioral aspects, as well as public health policies directed toward sexually transmitted infections, might have positively influenced the decline in HTLV-2 infections.
Seizures regulate the cation-Cl− cotransporter NKCC1 in a hamster model of epilepsy: implications for GABA neurotransmission
Authors: Bonet-Fernández Juan-Manuel , Tranque Pedro , Aroca-Aguilar Jose Daniel , Muñoz Luis J. , López Dolores E. , Escribano Julio , de Cabo Carlos
Published: June 2023

Background

The balance between the activity of the Na+/K+/Cl− cotransporter (NKCC1) that introduces Cl− into the cell and the K+/Cl− cotransporter (KCC2) that transports Cl− outside the cell is critical in determining the inhibitory or excitatory outcome of GABA release. Mounting evidence suggests that the impairment of GABAergic inhibitory neurotransmission plays a crucial role in the pathophysiology of epilepsy, both in patients and animal models. Previous studies indicate that decreased KCC2 expression is linked to audiogenic seizures in GASH/Sal hamsters, highlighting that Cl− imbalance can cause neuronal hyperexcitability. In this study, we aimed to investigate whether the Na+/K+/Cl− cotransporter NKCC1 is also affected by audiogenic seizures and could, therefore, play a role in neuronal hyperexcitability within the GASH/Sal epilepsy model.

Methods

NKCC1 protein expression in both the GASH/Sal strain and wild type hamsters was analyzed by immunohistochemistry and Western blotting techniques. Brain regions examined included cortex, hippocampus, hypothalamus, inferior colliculus and pons-medulla oblongata, which were evaluated both at rest and after sound-inducing seizures in GASH/Sal hamsters. A complementary analysis of NKCC1 gene slc12a2 expression was conducted by real-time PCR. Finally, protein and mRNA levels of glutamate decarboxylase GAD67 were measured as an indicator of GABA release.

Results

The induction of seizures caused significant changes in NKCC1 expression in epileptic GASH/Sal hamsters, despite the similar brain expression pattern of NKCC1 in GASH/Sal and wild type hamsters in the absence of seizures. Interestingly, the regulation of brain NKCC1 by seizures demonstrated regional specificity, as protein levels exclusively increased in the hippocampus and hypothalamus. Complementary real-time PCR analysis revealed that NKCC1 regulation was post-transcriptional only in the hypothalamus. In addition, seizures also modulated GAD67 mRNA levels in a brain region-specific manner. The increased GAD67 expression in the hippocampus and hypothalamus of the epileptic hamster brain suggests that NKCC1 upregulation overlaps with GABA release in these regions during seizures.

Conclusions

Our results indicate that seizure induction causes dysregulation of NKCC1 expression in GASH/Sal animals, which overlaps with changes in GABA release. These observations provide evidence for the critical role of NKCC1 in how seizures affect neuronal excitability, and support NKCC1 contribution to the development of secondary foci of epileptogenic activity.
Sex-specific associations between daytime sleepiness, chronic diseases and mortality in obstructive sleep apnea
Authors: Covassin Naima , Lu Dongmei , St. Louis Erik K. , Chahal Anwar A. , Schulte Phillip J. , Mansukhani Meghna P. , Xie Jiang , Lipford Melissa C. , Li Nanfang , Ramar Kannan , Caples Sean M. , Gay Peter C. , Olson Eric J. , Silber Michael H. , Li Jingen , Somers Virend K.
Published: June 2023

Objective

Excessive daytime sleepiness (EDS) is common in obstructive sleep apnea (OSA) and has been linked to adverse outcomes, albeit inconsistently. Furthermore, whether the prognostic impact of EDS differs as a function of sex is unclear. We aimed to assess the associations between EDS and chronic diseases and mortality in men and women with OSA.

Methods

Newly-diagnosed adult OSA patients who underwent sleep evaluation at Mayo Clinic between November 2009 and April 2017 and completed the Epworth Sleepiness Scale (ESS) for assessment of perceived sleepiness (N = 14,823) were included. Multivariable-adjusted regression models were used to investigate the relationships between sleepiness, with ESS modeled as a binary (ESS > 10) and as a continuous variable, and chronic diseases and all-cause mortality.

Results

In cross-sectional analysis, ESS > 10 was independently associated with lower risk of hypertension in male OSA patients (odds ratio [OR], 95% confidence interval [CI]: 0.76, 0.69–0.83) and with higher risk of diabetes mellitus in both OSA men (OR, 1.17, 95% CI 1.05–1.31) and women (OR 1.26, 95% CI 1.10–1.45). Sex-specific curvilinear relations between ESS score and depression and cancer were noted. After a median 6.2 (4.5–8.1) years of follow-up, the hazard ratio for all-cause death in OSA women with ESS > 10 compared to those with ESS ≤ 10 was 1.24 (95% CI 1.05–1.47), after adjusting for demographics, sleep characteristics and comorbidities at baseline. In men, sleepiness was not associated with mortality.

Conclusion

The implications of EDS for morbidity and mortality risk in OSA are sex-dependent, with hypersomnolence being independently associated with greater vulnerability to premature death only in female patients. Efforts to mitigate mortality risk and restore daytime vigilance in women with OSA should be prioritized.
Review on performance of chromium-coated zirconium and its failure mechanisms
Authors: Li Ziyi , Wang Xiaomin , Chen Huan , Zhang Ruiqian , Wei Tianguo , Yao Lifu , Wang Pengfei
Published: June 2023

The conventional Zircaloy cladding may react with coolant in light water reactors and elicit severe accident at high temperature, which constraint the safe and efficient development of nuclear energy. To avoid the tragedy happened in Fukushima, the concept of Accident Tolerant Fuel (ATF) was developed around the globe to secure the steady operation for nuclear power plants. As a short-term solution, deposition of protective coatings upon the cladding tubes are proved to be effective. Among the coatings, Chromium is one of the most outstanding choices. This paper reviews the recent study results about the performance and failure mechanisms of Cr-coated Zr cladding, including mechanical, irradiation, high-temperature oxidation tests, etc. under normal and/or accident conditions. The experiments and tests collectively suggest that Cr coating possesses excellent comprehensive properties for integrity protection of claddings and provides a promising future of the commercial application in reactor cores. Finally, this review proposes ambiguities and questions needed to be analyzed and clarified in future works.
Targeting cyclin D1 as a therapeutic approach for papillary thyroid carcinoma
Authors: Cai Wei , Shu Lin-Zhen , Liu Ding-Jie , Zhou Lv , Wang Meng-Meng , Deng Huan
Published: June 2023

Cyclin D1 functions as a mitogenic sensor that specifically binds to CDK4/6, thereby integrating external mitogenic inputs and cell cycle progression. Cyclin D1 interacts with transcription factors and regulates various important cellular processes, including differentiation, proliferation, apoptosis, and DNA repair. Therefore, its dysregulation contributes to carcinogenesis. Cyclin D1 is highly expressed in papillary thyroid carcinoma (PTC). However, the particular cellular mechanisms through which abnormal cyclin D1 expression causes PTC are poorly understood. Unveiling the regulatory mechanisms of cyclin D1 and its function in PTC may help determine clinically effective strategies, and open up better opportunities for further research, leading to the development of novel PTC regimens that are clinically effective. This review explores the mechanisms underlying cyclin D1 overexpression in PTC. Furthermore, we discuss the role of cyclin D1 in PTC tumorigenesis via its interactions with other regulatory elements. Finally, recent progress in the development of therapeutic options targeting cyclin D1 in PTC is examined and summarized.
Deep convolutional neural networks for multiple histologic types of ovarian tumors classification in ultrasound images
Authors: Wu Meijing , Cui Guangxia , Lv Shuchang , Chen Lijiang , Tian Zongmei , Yang Min , Bai Wenpei
Published: June 2023

Objective

This study aimed to evaluate and validate the performance of deep convolutional neural networks when discriminating different histologic types of ovarian tumor in ultrasound (US) images.

Material and methods

Our retrospective study took 1142 US images from 328 patients from January 2019 to June 2021. Two tasks were proposed based on US images. Task 1 was to classify benign and high-grade serous carcinoma in original ovarian tumor US images, in which benign ovarian tumor was divided into six classes: mature cystic teratoma, endometriotic cyst, serous cystadenoma, granulosa-theca cell tumor, mucinous cystadenoma and simple cyst. The US images in task 2 were segmented. Deep convolutional neural networks (DCNN) were applied to classify different types of ovarian tumors in detail. We used transfer learning on six pre-trained DCNNs: VGG16, GoogleNet, ResNet34, ResNext50, DensNet121 and DensNet201. Several metrics were adopted to assess the model performance: accuracy, sensitivity, specificity, FI-score and the area under the receiver operating characteristic curve (AUC).

Results

The DCNN performed better in labeled US images than in original US images. The best predictive performance came from the ResNext50 model. The model had an overall accuracy of 0.952 for in directly classifying the seven histologic types of ovarian tumors. It achieved a sensitivity of 90% and a specificity of 99.2% for high-grade serous carcinoma, and a sensitivity of over 90% and a specificity of over 95% in most benign pathological categories.

Conclusion

DCNN is a promising technique for classifying different histologic types of ovarian tumors in US images, and provide valuable computer-aided information.
Case Report: Clinical application of continuous arterial infusion chemotherapy in neoadjuvant therapy for locally advanced gastric cancer
Authors: Lin Wenli , Huang Zhongxian , Du Zhenhua , Wang Yunshan , Zuo Taiyang
Published: June 2023

Platinum-fluorouracil combination chemotherapy is the standard neoadjuvant treatment for locally advanced gastric cancer in China, but it does not improve the survival benefit of patients. In recent years, the application of immune checkpoint inhibitors and/or targeted drugs in neoadjuvant therapy for gastric cancer has achieved certain efficacy, but the survival benefit of patients is still not obvious. Intra-arterial infusion chemotherapy, as a method of regional therapy, has been widely used in the treatment of many advanced tumors and achieved remarkable curative effect. The role of arterial infusion chemotherapy in neoadjuvant therapy for gastric cancer is not clear. We describe two patients with locally advanced gastric cancer treated with continuous arterial infusion neoadjuvant chemotherapy. Two patients received continuous arterial infusion of chemotherapy drugs for 50 hours, the drugs were pumped into the main feeding artery of the tumor through the arterial catheter. A total of 4 cycles were followed, then undergone surgical resection. The postoperative pathological pCR of two patients was 100%, TRG was 0 grade, and no further anti-tumor therapy was required after operation, achieving clinical cure. During the treatment period, no serious adverse events occurred in either patient. These results suggest that continuous arterial infusion chemotherapy may be a new adjuvant therapy for locally advanced gastric cancer.
Association of parent-child interactions with parental psychological distress and resilience during the COVID-19 pandemic
Authors: Mann Mana , Harary David , Louis Shirley , Wang Tao , Bonuck Karen , Isasi Carmen R. , Charron Maureen J. , Fuloria Mamta
Published: June 2023

Introduction

The effects of psychological distress/resilience on parent-child engagement (e.g., family dinners, reading) during the COVID-19 pandemic have not been well studied. Among very young children from underrepresented backgrounds enrolled in the ongoing longitudinal Bronx Mother Baby Health Study of healthy term infants, we (1) examined associations between exposures to COVID-19-related events, demographic factors and parental psychological distress and resilience; and (2) correlated these factors with parent-child engagement activities.

Methods

Between June 2020-August 2021, parents of 105 Bronx Mother Baby Health Study participants aged birth-25 months completed questionnaires related to exposures to COVID-19-related events, frequency of positive parent-child engagement activities, food and housing insecurity, and parental psychological distress and resilience. Families were also asked open ended questions about the pandemic's impact.

Results

29.8% and 47.6% of parents reported food and housing insecurity, respectively. Greater exposures to COVID-19-related events were associated with increased parental psychological distress. Positive parent-child interactions were associated with demographic factors and higher levels of maternal education, but not with exposures to COVID-19-related events.

Discussion

This study adds to a growing body of literature on the negative impacts of COVID-19 exposures and psychosocial stressors on families during the pandemic, supporting the need for enhanced mental health resources and social supports for families.
Twelve-year outcomes of bedside laser photocoagulation for severe retinopathy of prematurity
Authors: Yang Bingzhi , Lian Chaohui , Tian Ruyin , Chen Yi , Tang Song , Xiang Haishan , He Honghui , Zhang Guoming
Published: June 2023

Purpose

The purpose of this study is to evaluate the 12-year outcomes of bedside laser photocoagulation (LP) for severe retinopathy of prematurity (ROP) under sedation combined with ocular surface anesthesia in neonatal intensive care units (NICU).

Design

The study is a retrospective case series.

Methods

Infants treated with bedside LP for severe ROP from April 2009 to September 2021 were included. All LP treatments were performed under sedation and surface anesthesia at the bedside in NICU. Data were recorded for clinical and demographic characteristics, total laser spots, duration of treatment, proportion of total regression of ROP, proportion of recurrence, and adverse events.

Results

A total of 364 infants (715 eyes) were included, with a mean gestational age of 28.6 ± 2.4 weeks (range: 22.6–36.6 weeks) and a mean birth weight of 1,156.0 ± 339.0 g (range: 480–2,200 g). The mean number of laser spots was 832 ± 469, and the mean duration of treatment was 23.5 ± 5.3 min per eye. Of all the eyes, 98.3% responded to LP with complete regression of ROP. ROP recurred in 15 (2.1%) eyes after the initial LP. Additional LP was performed in seven (1.0%) eyes. No patient exhibited mistaken LP of other ocular tissues, and there were no serious ocular adverse effects. None of them needed endotracheal intubation.

Conclusions

Bedside LP treatment is effective and safe for premature infants with severe ROP under sedation and surface anesthesia in NICU, especially for infants whose general condition is unstable and not suitable for transport.
Assessment of interleukin-10 promoter variant (−1082A/G) and cytokine production in patients with hemolytic uremic syndrome
Authors: Mongelos Micaela Aldana , Sosa Fernando Nicolás , Pineda Gonzalo Ezequiel , Fiorentino Gabriela , Santiago Adriana , Abelleyro Miguel Martín , Rossetti Liliana Carmen , Exeni Ramón , De Brasi Carlos Daniel , Palermo Marina Sandra , Ramos María Victoria
Published: June 2023

Introduction

Hemolytic uremic syndrome (HUS) is a condition that results in acute kidney failure mainly in children, which is caused by Shiga toxin–producing Escherichia coli and inflammatory response. Although anti-inflammatory mechanisms are triggered, studies on the implication in HUS are scarce. Interleukin-10 (IL-10) regulates inflammation in vivo, and the interindividual differences in its expression are related to genetic variants. Notably, the single nucleotide polymorphism (SNP) rs1800896 −1082 (A/G), located in the IL-10 promoter, regulates cytokine expression.

Methods

Plasma and peripheral blood mononuclear cells (PBMC) were collected from healthy children and HUS patients exhibiting hemolytic anemia, thrombocytopenia, and kidney damage. Monocytes identified as CD14+ cells were analyzed within PBMC by flow cytometry. IL-10 levels were quantified by ELISA, and SNP −1082 (A/G) was analyzed by allele-specific PCR.

Results

Circulating IL-10 levels were increased in HUS patients, but PBMC from these patients exhibited a lower capacity to secrete this cytokine compared with those from healthy children. Interestingly, there was a negative association between the circulating levels of IL-10 and inflammatory cytokine IL-8. We observed that circulating IL-10 levels were threefold higher in HUS patients with −1082G allele in comparison to AA genotype. Moreover, there was relative enrichment of GG/AG genotypes in HUS patients with severe kidney failure.

Discussion

Our results suggest a possible contribution of SNP −1082 (A/G) to the severity of kidney failure in HUS patients that should be further evaluated in a larger cohort.
Exploring the mechanisms under Zuogui Pill’s treatment of ischemic stroke through network pharmacology and in vitro experimental verification
Authors: Li Li , Liu Yan , Zheng Yawei , Zhu Jian , Wu Dan , Yan Xiaohui , Li Changyin , Wu Minghua , Li Wenlei
Published: June 2023

Due to its high mortality, incidence and disability rates, ischemic stroke poses heavy economic burdens to families and society. Zuogui Pill (ZGP) is a classic Chinese medicine for tonifying the kidney, which is effective for the recovery of neurological function after ischemic stroke. However, Zuogui Pill has not been evaluated for its potential effects on ischemic strokes. Using network pharmacology, the research aimed to explore the mechanisms of Zuogui Pill on ischemic stroke, which were further validated in SH-SY5Y cells injured by oxygen and glucose deprivation/reperfusion (OGD/R). Network analysis of Zuogui Pill identified 86 active ingredients and 107 compound-related targets correlated with ischemic stroke. Additionally, 11 core active compounds were obtained, such as Quercetin, beta sitosterol, and stigmasterol. Most of the compounds have been proven to have pharmacological activities. Based on pathway enrichment studies, Zuogui Pill may exert neuroprotection through MAPK signaling, PI3K-Akt signaling and apoptosis, as well as enhance neurite outgrowth and axonal regeneration effect via mTOR signaling, p53 signaling and Wnt signaling pathways. In vitro experiment, the viability of ischemic neuron treated with Zuogui Pill was increased, and the ability of neurite outgrowth was significantly improved. Western blot assays shown that the pro-neurite outgrowth effect of Zuogui Pill on ischemic stroke may be relate to PTEN/mTOR signal pathway. The results of the study provided new insights into Zuogui Pill’s molecular mechanism in treatment of ischemic stroke, as well as clinical references for its use.
Comprehensively analysis of immunophenotyping signature in triple-negative breast cancer patients based on machine learning
Authors: Tang Lijuan , Zhang Zhe , Fan Jun , Xu Jing , Xiong Jiashen , Tang Lu , Jiang Yan , Zhang Shu , Zhang Gang , Luo Wentian , Xu Yan
Published: June 2023

Immunotherapy is a promising strategy for triple-negative breast cancer (TNBC) patients, however, the overall survival (OS) of 5-years is still not satisfactory. Hence, developing more valuable prognostic signature is urgently needed for clinical practice. This study established and verified an effective risk model based on machine learning methods through a series of publicly available datasets. Furthermore, the correlation between risk signature and chemotherapy drug sensitivity were also performed. The findings showed that comprehensive immune typing is highly effective and accurate in assessing prognosis of TNBC patients. Analysis showed that IL18R1, BTN3A1, CD160, CD226, IL12B, GNLY and PDCD1LG2 are key genes that may affect immune typing of TNBC patients. The risk signature plays a robust ability in prognosis prediction compared with other clinicopathological features in TNBC patients. In addition, the effect of our constructed risk model on immunotherapy response was superior to TIDE results. Finally, high-risk groups were more sensitive to MR-1220, GSK2110183 and temsirolimus, indicating that risk characteristics could predict drug sensitivity in TNBC patients to a certain extent. This study proposes an immunophenotype-based risk assessment model that provides a more accurate prognostic assessment tool for patients with TNBC and also predicts new potential compounds by performing machine learning algorithms.
Insights into the mechanisms governing P01 scorpion toxin effect against U87 glioblastoma cells oncogenesis
Authors: Mlayah-Bellalouna Saoussen , Aissaoui-Zid Dorra , Chantome Aurelie , Jebali Jed , Souid Soumaya , Ayedi Emna , Mejdoub Hafedh , Belghazi Maya , Marrakchi Naziha , Essafi-Benkhadir Khadija , Vandier Christophe , Srairi-Abid Najet
Published: June 2023

The emerging concept of small conductance Ca2+-activated potassium channels (SKCa) as pharmacological target for cancer treatment has significantly increased in recent years. In this study, we isolated the P01 toxin from Androctonus australis (Aa) scorpion venom and investigated its effect on biological properties of glioblastoma U87, breast MDA-MB231 and colon adenocarcinoma LS174 cancer cell lines. Our results showed that P01 was active only on U87 glioblastoma cells. It inhibited their proliferation, adhesion and migration with IC50 values in the micromolar range. We have also shown that P01 reduced the amplitude of the currents recorded in HEK293 cells expressing SK2 channels with an IC50 value of 3 pM, while it had no effect on those expressing SK3 channels. The investigation of the SKCa channels expression pattern showed that SK2 transcripts were expressed differently in the three cancer cell lines. Particularly, we highlighted the presence of SK2 isoforms in U87 cells, which could explain and rely on the specific activity of P01 on this cell line. These experimental data highlighted the usefulness of scorpion peptides to decipher the role of SKCa channels in the tumorigenesis process, and develop potential therapeutic molecules targeting glioblastoma with high selectivity.
Predicting disordered regions driving phase separation of proteins under variable salt concentration
Authors: Meca Esteban , Fritsch Anatol W. , Iglesias-Artola Juan M. , Reber Simone , Wagner Barbara
Published: June 2023

We investigate intrinsically disordered regions (IDRs) of phase separating proteins regarding their impact on liquid-liquid phase separation (LLPS) of the full protein. Our theoretical approach uses a mean-field theory that accounts for sequence-dependent electrostatic interactions via a Random Phase Approximation (RPA) and in addition allows for variable salt concentration for the condensed and dilute protein phases. The numerical solution of the complete phase diagrams together with the tie lines that we derive for this model system leaves two parameters to be determined by fitting experimental data on concentrations of all species involved in the system. For our comparisons, we focus on two proteins, PGL-3 and FUS, known to undergo LLPS. For PGL-3 we predict that its long IDR near the C-terminus promotes LLPS, which we validate through direct comparison with in vitro experimental results under the same physiological conditions. For the structurally more complex protein FUS the role of the low complexity (LC) domain in LLPS has been intensively studied. Apart from the LC domain we here investigate theoretically two IDRs, one near the N-terminus and another near the C-terminus. Our theoretical analysis of these domains predict that the IDR at the N-terminus (aa 1-285) is the main driver of LLPS of FUS by comparison to in vitro experiments of the full length protein under the same physiological temperature and salt conditions.
Multi-wavelength SPAD photoplethysmography for cardio-respiratory monitoring
Authors: Cusini Iris , Rinaldi Riccardo , Castiglioni Paolo , Faini Andrea , Villa Federica
Published: June 2023

There is a growing interest in photoplethysmography (PPG) for the continuous monitoring of cardio-respiratory signals by portable instrumentation aimed at the early diagnosis of cardiovascular diseases. In this context, it is conceivable that PPG sensors working at different wavelengths simultaneously can optimize the identification of apneas and the quantification of the associated heart-rate changes or other parameters that depend on the PPG shape (e.g., systematic vascular resistance and pressure), when evaluating the severity of breathing disorders during sleep and in general for health monitoring. Therefore, the objective of this work is to present a novel pulse oximeter that provides synchronous data logging related to three light wavelengths (green, red, and infrared) in transmission mode to optimize both heart rate measurements and a reliable and continuous assessment of oxygen saturation. The transmission mode is considered more robust over motion artifacts than reflection mode, but current pulse oximeters cannot employ green light in transmission mode due to the high absorbance of body tissues at this wavelength. For this reason, our device is based on a Single-Photon Avalanche Diode (SPAD) with very short deadtime (less than 1 ns) to have, at the same time, the single photon sensitivity and high-count rate that allows acquiring all the wavelengths of interest on the same site and in transmission mode. Previous studies have shown that SPAD cameras can be used for measuring the heart rate through remote PPG, but oxygen saturation and heart-rate measures through contact SPAD-based PPG sensors have never been addressed so far. The results of the preliminary validation on six healthy volunteers reflect the expected physiological phenomena, providing rms errors in the Inter Beat Interval estimation smaller than 70 ms (with green light) and a maximum error in the oxygen saturation smaller than 1% during the apneas. Our prototype demonstrates the reliability of SPAD-based devices for continuous long-term monitoring of cardio-respiratory variables as an alternative to photodiodes especially when minimal area and optical power are required.
Effect of estradiol and predator cues on behavior and brain responses of captive female house sparrows (Passer domesticus)
Authors: Kimball Melanie G. , Harding Courtney T. , Couvillion Kaitlin E. , Stansberry Keegan R. , Kelly Tosha R. , Lattin Christine R.
Published: June 2023

The presence of predators can cause major changes in animal behavior, but how this interacts with hormonal state and brain activity is poorly understood. We gave female house sparrows (Passer domesticus) in post-molt condition an estradiol (n = 17) or empty implant (n = 16) for 1 week. Four weeks after implant removal, a time when female sparrows show large differences in neuronal activity to conspecific vs. heterospecific song, we exposed birds to either 30 min of conspecific song or predator calls, and video recorded their behavior. Females were then euthanized, and we examined neuronal activity using the expression of the immediate early gene (IEG) ZENK to identify how the acoustic stimuli affected neuronal activation. We predicted that if female sparrows with estradiol implants reduce neuronal activity in response to predator calls as they do to neutral tones and non-predatory heterospecifics, they would show less fear behavior and a decreased ZENK response in brain regions involved in auditory (e.g., caudomedial mesopallium) and threat perception functions (e.g., medial ventral arcopallium) compared to controls. Conversely, we predicted that if females maintain auditory and/or brain sensitivity towards predator calls, then female sparrows exposed to estradiol would not show any differences in ZENK response regardless of playback type. We found that female sparrows were less active during predator playbacks independent of hormone treatment and spent more time feeding during conspecific playback if they had previously been exposed to estradiol. We observed no effect of hormone or sound treatment on ZENK response in any region of interest. Our results suggest that female songbirds maintain vigilance towards predators even when in breeding condition.
A temporal analysis and response to nitrate availability of 3D root system architecture in diverse pennycress (Thlaspi arvense L.) accessions
Authors: Griffiths Marcus , Liu Alexander E. , Gunn Shayla L. , Mutan Nida M. , Morales Elisa Y. , Topp Christopher N.
Published: June 2023

Introduction

Roots have a central role in plant resource capture and are the interface between the plant and the soil that affect multiple ecosystem processes. Field pennycress (Thlaspi arvense L.) is a diploid annual cover crop species that has potential utility for reducing soil erosion and nutrient losses; and has rich seeds (30-35% oil) amenable to biofuel production and as a protein animal feed. The objective of this research was to (1) precisely characterize root system architecture and development, (2) understand plastic responses of pennycress roots to nitrate nutrition, (3) and determine genotypic variance available in root development and nitrate plasticity.

Methods

Using a root imaging and analysis pipeline, the 4D architecture of the pennycress root system was characterized under four nitrate regimes, ranging from zero to high nitrate concentrations. These measurements were taken at four time points (days 5, 9, 13, and 17 after sowing).

Results

Significant nitrate condition response and genotype interactions were identified for many root traits, with the greatest impact observed on lateral root traits. In trace nitrate conditions, a greater lateral root count, length, density, and a steeper lateral root angle was observed compared to high nitrate conditions. Additionally, genotype-by-nitrate condition interaction was observed for root width, width:depth ratio, mean lateral root length, and lateral root density.

Discussion

These findings illustrate root trait variance among pennycress accessions. These traits could serve as targets for breeding programs aimed at developing improved cover crops that are responsive to nitrate, leading to enhanced productivity, resilience, and ecosystem service.
How do you build a nectar spur? A transcriptomic comparison of nectar spur development in Linaria vulgaris and gibba development in Antirrhinum majus
Authors: Cullen Erin , Wang Qi , Glover Beverley J.
Published: June 2023

Nectar spurs (tubular outgrowths of floral organs) have long fascinated biologists. However, given that no model species possess nectar spurs, there is still much to learn about their development. In this study we combined morphological analysis with comparative transcriptomics to gain a global insight into the morphological and molecular basis of spur outgrowth in Linaria. Whole transcriptome sequencing was performed on two related species at three key developmental stages (identified by our morphological analysis), one with a spur (Linaria vulgaris), and one without a spur (Antirrhinum majus). A list of spur-specific genes was selected, on which we performed a gene enrichment analysis. Results from our RNA-seq analysis agreed with our morphological observations. We describe gene activity during spur development and provide a catalogue of spur-specific genes. Our list of spur-specific genes was enriched for genes connected to the plant hormones cytokinin, auxin and gibberellin. We present a global view of the genes involved in spur development in L. vulgaris, and define a suite of genes which are specific to spur development. This work provides candidate genes for spur outgrowth and development in L. vulgaris which can be investigated in future studies.
Lipid droplets are versatile organelles involved in plant development and plant response to environmental changes
Authors: Bouchnak Imen , Coulon Denis , Salis Vincent , D’Andréa Sabine , Bréhélin Claire
Published: June 2023

Since decades plant lipid droplets (LDs) are described as storage organelles accumulated in seeds to provide energy for seedling growth after germination. Indeed, LDs are the site of accumulation for neutral lipids, predominantly triacylglycerols (TAGs), one of the most energy-dense molecules, and sterol esters. Such organelles are present in the whole plant kingdom, from microalgae to perennial trees, and can probably be found in all plant tissues. Several studies over the past decade have revealed that LDs are not merely simple energy storage compartments, but also dynamic structures involved in diverse cellular processes like membrane remodeling, regulation of energy homeostasis and stress responses. In this review, we aim to highlight the functions of LDs in plant development and response to environmental changes. In particular, we tackle the fate and roles of LDs during the plant post-stress recovery phase.
Anxiety-associated factors among employees with different personality profiles: a cross-sectional study in China
Authors: Hao Ran , Zuo Jinfan , Jin Haoyu , Wang Yipeng , Zhang Lei , Zhu Yufang , Wang Ye , Qi Yixin , Qi Jiale , Xia Jing , Wu Yibo , Hu Jie
Published: June 2023

Introduction

Anxiety not only harms employees’ work efficiency and satisfaction but also presents as a hazard to their mental health. This study aimed to investigate the prevalence of anxiety among Chinese employees, identify their personality profiles and explore the anxiety-related factors in different personality profiles.

Methods

This national investigation adopted the multistage random sampling method to recruit employees. A total of 3,875 employees were enrolled in this study, and 39.1% (1,515/3,875) of them were experiencing anxiety at the time of the study. Latent profile analysis (LPA) was conducted to identify personality subgroups among Chinese employees based on their BFI-10 scores.

Results

LPA identified a three-profile solution among Chinese employees: average, resilient, and introverted. Employees in the resilient profile had the lowest anxiety rate (16.1%, 132/822), and those in the average profile had the highest rate (46.8%, 1,166/2,494). Multivariate analysis results showed that for all personality profiles, self-efficacy was positively associated with anxiety, and work-family conflict was negatively associated with anxiety. High levels of perceived social support and self-efficacy reduced the risk of anxiety and higher work-family conflict and no partner increased the odds of anxiety in the average profile. For the introverted profile, female gender, and living in a city increased the chances of suffering from anxiety.

Discussion

This study identified that each personality profile of Chinese employees had its own set of factors associated with anxiety, which could facilitate employers to provide targeted interventions to alleviate employees’ anxiety.
Assessing affect in adolescents with e-diaries: multilevel confirmatory factor analyses of different factor models
Authors: Limberger Matthias F. , Schmiedek Florian , Santangelo Philip S. , Reichert Markus , Wieland Lena M. , Berhe Oksana , Meyer-Lindenberg Andreas , Tost Heike , Ebner-Priemer Ulrich W.
Published: June 2023

In the last two decades, e-diary studies have gained increasing interest, with a dominant focus on mood and affect. Although requested in current guidelines, psychometric properties are rarely reported, and methodological investigations of factor structure, model fit, and the reliability of mood and affect assessment are limited. We used a seven-day e-diary dataset of 189 adolescent participants (12–17  years). The e-diary affect assessments revealed a considerable portion of within-person variance. The six-factor model showed the best model fit compared to the less complex models. Factor loadings also improved with the complexity of the models. Accordingly, we recommend that future e-diary studies of adolescents use the six-factor model of affect as well as reporting psychometric properties and model fit. For future e-diary scale development, we recommend using a minimum of three items per scale to enable the use of confirmatory multilevel factor analyses.
Tone and word length across languages
Authors: Wichmann Søren
Published: June 2023

The aim of this paper is to show evidence of a statistical dependency of the presence of tones on word length. Other work has made it clear that there is a strong inverse correlation between population size and word length. Here it is additionally shown that word length is coupled with tonal distinctions, languages being more likely to have such distinctions when they exhibit shorter words. It is hypothesized that the chain of causation is such that population size influences word length, which, in turn, influences the presence and number of tonal distinctions.
Applying ecological systems theory to juvenile legal system interventions outcomes research: a measurement framework
Authors: Sheerin Kaitlin M. , Brodell Regina , Huey Stanley J. , Kemp Kathleen A.
Published: June 2023

Intervention research and development for youth in the juvenile legal system (JLS) has often focused on recidivism as the primary outcome of interest. Although recidivism is an important outcome, it is ultimately a downstream marker of success and is affected by changes in other domains of youths’ lives (e.g., family and peer relations, neighborhood safety, local and state-level policies). Thus, the present manuscript proposes the application of ecological systems theory to selecting outcomes to assess intervention effects in JLS intervention research to better capture proximal and distal influences on youth behavior. To that end, we first provide an overview of the strengths and limitations of using recidivism as an outcome measure. Next, the current application of social ecology theory to existing research on both risk and protective factors of JLS involvement is discussed, as well as existing work on assessing social-ecological domains within intervention studies. Then, a measurement framework is introduced for selecting pertinent domains of youths’ social ecologies to assess as intervention outcomes, moderators, and mediators. To facilitate this, we provide examples of concrete constructs and measures that researchers may select. We conclude with potential new avenues of research to which our proposed framework could lead, as well as potential limitations of implementing our framework.
IoT-enabled user agency for building sustainability
Authors: Lee Stephen , Dieng Ousmane , Shangguan Longfei , Biehl Jacob , Lee Adam , Mossé Daniel , Chrysanthis Panos K.
Published: June 2023

Buildings are a significant source of greenhouse gas emissions, and many organizations recognize that reducing commercial building-related emissions is crucial to achieving a carbon-neutral future. However, leveraging state-of-the-art building energy-efficiency techniques can be expensive and require installing new equipment, resulting in only a small percentage of energy-efficient commercial buildings. To address this challenge, our paper focuses on empowering building occupants to become informed change-makers that drive building efficiency through an IoT-based solution. By leveraging the data collected from low-cost sensors, we envision creating personalized dashboards and interventions for individuals, fostering energy transparency and empowering people to optimize their space usage while reducing emissions. Moreover, we can leverage human flexibility and flexible environments to further improve space usage and realize energy and emissions reduction opportunities. Thus, by enabling human-building interaction, our approach will be more effective in achieving the full potential of reducing energy and emissions in existing buildings.
Deep neural networks of solar flare forecasting for complex active regions
Authors: Li Ming , Cui Yanmei , Luo Bingxian , Wang Jingjing , Wang Xin
Published: June 2023

Solar flare forecasting is one of major components of operational space weather forecasting. Complex active regions (ARs) are the main source producing major flares, but only a few studies are carried out to establish flare forecasting models for these ARs. In this study, four deep learning models, called Complex Active Region Flare Forecasting Model (CARFFM)-1, −2, −3, and −4, are established. They take AR longitudinal magnetic fields, AR vector magnetic fields, AR longitudinal magnetic fields and the total unsigned magnetic flux in the neutral line region, AR vector magnetic fields and the total unsigned magnetic flux in the neutral region as input, respectively. These four models can predict the production of M-class or above flares in the complex ARs for the next 48 h. Through comparing the performance of the models, CARFFM-4 has the best forecasting ability, which has the most abundant input information. It is suggested that more valuable and rich input can improve the model performance.
Socio-technical transitions and sustainable agriculture in Latin America and the Caribbean: a systematic review of the literature 2010–2021
Authors: Durán Yuri , Gómez-Valenzuela Víctor , Ramírez Katerin
Published: June 2023

The challenges and opportunities Latin American and Caribbean (LAC) countries face to meet sustainable development force nations to seek technological alternatives to ensure better policy design. It also includes technology transfer for the productive inclusion of the rural population in the region. This paper aims to characterize the conceptual frameworks applied to studying socio-technical transitions related to sustainable agriculture in the region. A systematic review literature (SRL) was conducted covering 2010–2021. The main findings suggest that the general ideas of socio-technical transition have been used to study sustainable agriculture in LAC. However, its use has been more implicit than explicit, with some predominance of the Strategic Niche Management (SNM) and the Transition Management Approach (TM) frameworks. In addition, the socio-technical transitions as a straightforward approach have started to be incorporated more clearly after 2020. Finally, the leading technologies to foster socio-technical transitions to sustainable agriculture in the region are related to pest control and soil conservation, so social practices such as certifications have had preponderance in this transition. This paper contributes to the existing literature, broadens the frontier of socio-technical analysis in the transition to sustainable agriculture, and expands our knowledge on applying socio-technical analysis in marginal contexts.
Effect of sun-dry on nutritional and sensory acceptability of wilted African leafy vegetables: a case study of Morogoro Region, Tanzania
Authors: Ahmed Amina , Almas Saliath , Kissaka Kelvin , Suleiman Rashid
Published: June 2023

African leafy vegetables are rich in micronutrients that can help solve hidden hunger problems in Sub-Saharan Africa. However, their availability is limited due to seasonality and high post-harvest losses, which account for about 50%. In the field survey, we observed that wilted vegetables are usually discarded from the Morogoro Region, Tanzania markets. That loss also contributes to the loss of resources used to cultivate and transport the vegetables. Therefore, this study aimed to investigate the effect of the commonly used sun-drying method on the nutritional and sensory properties of fresh and wilted African nightshade and cowpea leaves. The common practice of handling fresh vegetables by vendors was adopted by putting the freshly harvested vegetables in an open basin at 24 ± 2°C for 3 days. Then, the stored vegetables were either sprayed or dipped in water to reduce the wilting rate. Next, the fresh and the wilted vegetables were dipped into hot water at 100°C for 7 min. Afterwards, the vegetables were sundried (22.5 ± 5.5°C) for three consecutive days. To study the effect of wilting, blanching, and sun drying fresh and dried vegetables were analysed for moisture, ascorbic acid, β-carotene, antioxidant activity and nitrate contents. Fresh and dried vegetables were used to prepare sauces using traditional recipes. The vegetable sauces were analysed for their sensory attributes using 30 semi-trained panellists. After drying, ascorbic acid contents, antioxidant activity and nitrate were reduced. The β-carotene significantly increased in the African nightshade and decreased in the cowpea leaves. Ascorbic acid ranged from 17.40 to 2.22 mg/100 g, antioxidant activity from 37.30 to 19.78, nitrate 230 to 10 mg/100 g and β-carotene from 96.38 to 79.49 mg/100. The sensory results showed relative acceptability among the sauces prepared from the fresh, fresh-dried and wilted-dried vegetables. In conclusion, blanching before drying reduced the water-soluble nutrient content but improved the sauces’ sensory scores of fresh and wilted vegetables. Therefore, reducing the post-harvest loss of African leafy vegetables is possible by drying the wilted vegetables and preparing the sauces with acceptable quality.