bluuebunny/crossref_metadata_2025 huggingface dataset:
Hydrotreating of Waste Tire Pyrolysis Oil over Highly Dispersed Ni2P Catalyst Supported on SBA-15
Authors: Yun Gwang-Nam , Kim Ki-Duk , Lee Yong-Kul
Published: October 2021

A highly dispersed nickel phosphide catalyst supported on SBA-15 was prepared and tested for the hydrotreating of tire pyrolysis oil (TPO). Physicochemical properties of the prepared catalyst were analyzed by CO uptake chemisorption, BET, TEM, and X-ray diffraction (XRD). An advanced technique with gas chromatography equipped with mass spectrometry and atomic emission detector was applied to investigate carbon-, sulfur-, and nitrogen-containing compounds in TPO. Hydrotreating tests were carried out in a fixed-bed continuous flow reactor at 350 °C, 3.0 MPa, and LHSV of 0.5 h−1. The Ni2P/SBA-15 exhibited an HDS conversion of 89.3% and an HDN conversion of 60.7%, which was comparable to the performance of a commercial NiMoS catalyst under the same conditions.
The Unique Properties of Placental Mesenchymal Stromal Cells: A Novel Source of Therapy for Congenital and Acquired Spinal Cord Injury
Authors: Kulubya Edwin S , Clark Kaitlin , Hao Dake , Lazar Sabrina , Ghaffari-Rafi Arash , Karnati Tejas , Ebinu Julius Okudu , Zwienenberg Marike , Farmer Diana L , Wang Aijun
Published: October 2021

Spinal cord injury (SCI) is a devasting condition with no reliable treatment. Spina bifida is the most common cause of congenital SCI. Cell-based therapies using mesenchymal stem/stromal cells (MSCS) have been largely utilized in SCI. Several clinical trials for acquired SCI use adult tissue-derived MSC sources, including bone-marrow, adipose, and umbilical cord tissues. The first stem/stromal cell clinical trial for spina bifida is currently underway (NCT04652908). The trial uses early gestational placental-derived mesenchymal stem/stromal cells (PMSCs) during the fetal repair of myelomeningocele. PMSCs have been shown to exhibit unique neuroprotective, angiogenic, and antioxidant properties, all which are promising applications for SCI. This review will summarize the unique properties and current applications of PMSCs and discuss their therapeutic role for acquired SCI.
Loss of Endothelial Barrier Function in the Inflammatory Setting: Indication for a Cytokine-Mediated Post-Transcriptional Mechanism by Virtue of Upregulation of miRNAs miR-29a-3p, miR-29b-3p, and miR-155-5p
Authors: Maucher Daniel , Schmidt Birte , Schumann Julia
Published: October 2021

Dysfunction of the endothelial barrier plays a central role in the pathogenesis of both acute and chronic inflammatory processes such as sepsis or atherosclerosis. Due to attenuation of endothelial cell contacts, there is an increased transfer of blood proteins and fluid into the surrounding tissue, which relates to edema formation and distribution disorders. However, the mechanisms underlying these responses are not fully understood. In this study, we used human endothelial cells to mimic the loss of barrier function in an inflammatory milieu. We found that a weakened endothelial barrier after cytokine stimulation was accompanied by a significantly changed transcriptome. Apparent was a depletion of mRNAs encoding cell adhesion molecules. Furthermore, we found that cytokine treatment of endothelial cells induced upregulation of miR-29a-3p, miR-29b-3p, and miR-155-5p. miRNAs are known to negatively affect stability and translational efficiency of target mRNAs. Remarkably, miR-29a-3p, miR-29b-3p, and miR-155-5p have already been described to target the mRNAs of central tight and adherent junction proteins including F11 receptor, claudin 1, β-catenin, p120-catenin, and eplin. This taken together points to the existence of a posttranscriptional mechanism for expression inhibition of central adhesion proteins, which is triggered by inflammatory cytokines and mediated by miR-29a-3p, miR-29b-3p, and miR-155-5p.
From Hematopoietic Stem Cell Transplantation to Chimeric Antigen Receptor Therapy: Advances, Limitations and Future Perspectives
Authors: Voynova Elisaveta , Kovalovsky Damian
Published: October 2021

Chimeric antigen receptor (CAR) T-cell therapy was envisioned as a mechanism to re-direct effector T-cells to eliminate tumor cells. CARs are composed of the variable region of an antibody that binds a native cancer antigen coupled to the signaling domain of a TCR and co-stimulatory molecules. Its success and approval by the U.S. Food and Drug Administration for the treatment of B-cell malignancies revolutionized the immunotherapy field, leading to extensive research on its possible application for other cancer types. In this review, we will focus on the evolution of CAR-T cell therapy outlining current technologies as well as major obstacles for its wide application. We will highlight achievements, the efforts to increase efficacy and to evolve into an off-the-shelf treatment, and as a possible future treatment for non-cancer related diseases.
Optimization of Personalized Amlodipine Dosing Strategies for Children Based on Pharmacokinetic Data from Chinese Male Adults and PBPK Modeling
Authors: Han Xiaolu , Hong Xiaoxuan , Li Xianfu , Wang Yuxi , Wang Zengming , Zheng Aiping
Published: October 2021

For children, a special population who are continuously developing, a reasonable dosing strategy is the key to clinical therapy. Accurate dose predictions can help maximize efficacy and minimize pain in pediatrics. Methods: This study collected amlodipine pharmacokinetics (PK) data from 236 Chinese male adults and established a physiological pharmacokinetic (PBPK) model for adults using GastroPlus™. A PBPK model of pediatrics is constructed based on hepatic-to-body size and enzyme metabolism, used similar to the AUC0-∞ to deduce the optimal dosage of amlodipine for children aged 1–16 years. A curve of continuous administration for 2-, 6-, 12-, 16-, and 25-year-olds and a personalized administration program for 6-year-olds were developed. Results: The results show that children could not establish uniform allometric amplification rules. The optimal doses were 0.10 mg·kg−1 for ages 2–6 years and −0.0028 × Age + 0.1148 (mg/kg) for ages 7–16 years, r = 0.9941. The trend for continuous administration was consistent among different groups. In a 6-year-old child, a maintenance dose of 2.30 mg was used to increase the initial dose by 2.00 mg and the treatment dose by 1.00 mg to maintain stable plasma concentrations. Conclusions: A PBPK model based on enzyme metabolism can accurately predict the changes in the pharmacokinetic parameters of amlodipine in pediatrics. It can be used to support the optimization of clinical treatment plans in pediatrics.
The Multiscale Dynamics of the 29 June 2012 Super Derecho
Authors: Shourd Kacie Nicole , Kaplan Michael L.
Published: October 2021

The 29–30 June 2012 “super” derecho was, up until the 10 August 2020 “Iowa Derecho”, the most prolific derecho of modern times. While many of the synoptic-scale precursors to derecho events are understood, the multi-scale dynamics which likely distinguish derecho-producing events versus non-derecho events remain much more elusive. Using both observations and high-resolution WRF-ARW simulations, the sequence of adjustments that ultimately set up the pre-29 June derecho environment are examined. Planetary scale Rossby wave breaking occurred almost exactly two weeks before the super derecho on 15–16 June 2012 resulting in the development and intensification of a strong high-pressure system and mixed layer over the complex terrain of the western United States. A week after the initial Rossby wave break (~23 June), daily record-breaking temperatures began to dominate much of the central U.S. as the mixed layer/high pressure continued to strengthen. A second Rossby wave break on 26 June was crucial for detaching the mixed layer from the western U.S. elevated plateau, creating an elevated mixed layer that was rapidly deformed and propagated downstream to set up the derecho environment between 27–29 June. On 28 June, flow imbalance at the elevated mixed layer front resulted in highly ageostrophic circulations in the mid-levels, generating an along-stream mid-level jetlet which ultimately moved the elevated mixed layer and associated mesoscale front downstream across the Midwest and Mid-Atlantic. On the morning of 29 June, a well-defined corridor of both potential static instability and lowered inertial stability was set up across the Midwest and Mid-Atlantic states. This along with strong capping, a divergent polar jet entrance region to the north, and the highly imbalanced mid-level jetlet set the stage for this prolific severe convective event.
The Potential Role of Climate Indices to Explain Floods, Mass-Movement Events and Wildfires in Southern Italy
Authors: Coscarelli Roberto , Aguilar Enric , Petrucci Olga , Vicente-Serrano Sergio M. , Zimbo Fabio
Published: October 2021

Climate variability can be the source of several multiple hazards and damaging phenomena, such as flash floods, debris flows, landslides, forest fires, etc. In this study the response in the frequency of landslides, floods and forest fires to a set of climate indices is studied, referring to a region of southern Italy (Calabria) located in the center of the Mediterranean basin, a hot-spot for climate change. For these comparisons, 5022 landslides and 1584 flood occurrences for a 29-year period (1990–2018) have been selected for the whole Calabria; the burnt areas have been analyzed for the same territory from 2008 to 2018. The climate indices have been calculated by means of daily rainfall and temperature data registered in 93 stations. The results showed that landslide occurrences are more linked with climate indices describing not very intense rainfall. Conversely, floods show best matches with climate indices representative of more extreme precipitation. Regarding the burnt areas, the results confirmed that very dry climate conditions, modifying the moisture content of the soil, can change the intensity and the extension of fires.
Design of Metal-Organic Polymers MIL-53(M3+): Preparation and Characterization of MIL-53(Fe) and Graphene Oxide Composite
Authors: Nguyen Quang K. , Kuz’micheva Galina M. , Khramov Evgeny V. , Svetogorov Roman D. , Chumakov Ratibor G. , Cao Thuy T.
Published: October 2021

This article presents a crystal chemical analysis, generalization, and systematization of structural characteristics of metal-organic polymers MIL-53(M3+) with M = Al, Cr, Ga, and Fe. The division of the MIL-53(M3+) structures into a morphotropic series was performed, which made it possible to predict the formation of new compounds or solid solutions with the corresponding composition and structure. The change in the symmetry of MIL-53(M3+) and the causes of polymorphs formation are explained on the basis of crystal chemical rules. The efficiency of the revealed regularities in the structural characteristics of the MIL-53(M3+) phases were experimentally confirmed for MIL-53(Fe) and composite MIL-53(Fe)/GO (GO-graphene oxide) by several methods (powder X-ray, X-ray absorption, and photoelectron spectroscopy). For the first time, different coordination numbers (CN) (CNFe = 4.9 for MIL-53(Fe)—two types of coordination polyhedra with CNFe = 6 and CNFe = 4; CNFe = 4 for MIL-53 (Fe3+)/GO) and the formal charges (FC) of iron ions (variable FC of Fe (2+δ)+ in MIL-53(Fe3+) and Fe2+ in MIL-53(Fe3+)/GO) were found. These experimental data explain the higher photocatalytic activity of MIL-53(Fe3+)/GO in photo-Fenton reactions—RR195 decomposition.
Real Space Quantum Cluster Formulation for the Typical Medium Theory of Anderson Localization
Authors: Tam Ka-Ming , Terletska Hanna , Berlijn Tom , Chioncel Liviu , Moreno Juana
Published: October 2021

We develop a real space cluster extension of the typical medium theory (cluster-TMT) to study Anderson localization. By construction, the cluster-TMT approach is formally equivalent to the real space cluster extension of the dynamical mean field theory. Applying the developed method to the 3D Anderson model with a box disorder distribution, we demonstrate that cluster-TMT successfully captures the localization phenomena in all disorder regimes. As a function of the cluster size, our method obtains the correct critical disorder strength for the Anderson localization in 3D, and systematically recovers the re-entrance behavior of the mobility edge. From a general perspective, our developed methodology offers the potential to study Anderson localization at surfaces within quantum embedding theory. This opens the door to studying the interplay between topology and Anderson localization from first principles.
Biodiversity and Transportation Infrastructure in the Republic of Korea: A Review on Impacts and Mitigation in Developing the Country
Authors: Andersen Desiree , Jang Yikweon
Published: October 2021

The construction and continued use of transportation infrastructure, specifically roads, has had a significant global impact on biodiversity and the environment. The Republic of Korea, or South Korea, has a road density of 1.13 km/km2. So far, three nationwide studies about vertebrate road-killed species have been reported, showing bias towards medium to large mammals, the most common victims being the Korean water deer (Hydropotes inermis), Korean hare (Lepus coreanus), Siberian roe deer (Capreolus pygargus), and the common raccoon dog (Nyctereutes procyonoides). Road-kills, or wildlife-vehicle collisions (WVCs), tend to occur in or near preferred habitat types or in highly fragmented areas, with roads additionally being linked to habitat fragmentation and loss. Alongside WVCs and habitat effects, information about other adverse effects on biodiversity is scant, although there are reports that heavy metals and other pollutants from road runoff impact marine biodiversity, vegetation, soil, and groundwater. Furthermore, roads have been linked to a prevalence of invasive plant species. To mitigate road impacts, the South Korean government has constructed, with mixed results, 530 wildlife crossing structures, mainly including overpasses and tunnels. To mitigate road impacts more effectively, the country will need more construction, monitoring, and consistent management of wildlife crossing structures. Further, incorporating plans for wildlife crossing structures in early stages of road development will be required.
Platelet Activation and Inflammation in Patients with Papillary Thyroid Cancer
Authors: Martin Sorina , Mustata Theodor , Enache Oana , Ion Oana , Chifulescu Andreea , Sirbu Anca , Barbu Carmen , Miron Adrian , Giulea Cosmin , Andrei Florin , Fica Simona
Published: October 2021

Background: The primary endpoint was to analyze the preoperatory inflammatory markers and platelet indices in papillary thyroid cancer (PTC) patients compared with patients with benign thyroid pathology. The secondary endpoints were to analyze the relationship between these markers and the pathological features of PTC and to compare their pre- and postoperative levels in PTC patients. Methods: In this retrospective case-control study, we analyzed the files of 1183 patients submitted to thyroidectomy between January 2012 and December 2018. A total of 234 patients with PTC (mean age 51.54 ± 13.10 years, 84.6% females) were compared with an age-, gender- and BMI-matched control group of 108 patients with histologic benign thyroid disorders. Results: PTC patients had higher platelet count (PLT) (p = 0.011), plateletcrit (PCT) (p = 0.006), neutrophil (p = 0.022) and fibrinogen (p = 0.005) levels. Subgroup analysis showed that PTC females had higher PLT (p = 0.006), PCT (p < 0.001) and erythrocyte sedimentation rate (ESR) (p = 0.005), while males had higher neutrophil (p = 0.040) levels. Papillary thyroid cancer patients under 55 years had higher PLT (p < 0.001) and PCT (p = 0.010), while patients over 55 years had higher mean platelet volume (p = 0.032), neutrophil-to-lymphocyte ratio (p = 0.013), ESR (p = 0.005) and fibrinogen (p = 0.019) levels. Preoperative values for platelet indices and inflammatory markers were similar to the postoperative determinations in PTC patients. Fibrinogen (AUROC = 0.602, p = 0.02; cut-off = 327.5 mg/dL, Se = 53.8%, Sp = 62.9%) and PLT (AUROC = 0.584, p = 0.012; cut-off = 223.5 × 103/mm3, Se = 73.1%, Sp = 42.6%) were independent predictors of the presence of PTC. Conclusions: Our data show that fibrinogen and platelet count could be promising, inexpensive, independent predictors for the presence of PTC when compared with benign thyroid disorders.
Knee Capsule Anatomy: An MR Imaging and Cadaveric Study
Authors: Zibis Aristeidis H. , Vassalou Evangelia E. , Raoulis Vasileios A. , Lampridis Vasileios , Klontzas Michail E. , Fyllos Apostolos , Stavlas Panagiotis , Karantanas Apostolos H.
Published: October 2021

This research focuses on the anatomical insertion of the synovial capsule around the knee. The attachments of the capsule were measured in 50 knee MR imaging studies with large intraarticular effusion. Corresponding measurements were performed in 20 fresh frozen cadaveric specimens, for validation. Femoral and tibial capsular reflections were defined as the distances between the attachment sites of the capsule and the femoral or tibial joint line and they were recorded in three coronal planes (anterior/middle/posterior). On MR imaging, the lateral/medial femoral capsular reflection mean values were 6.5/4.57 cm, 2.74/1.74 cm and 1.52/1.99 cm in the anterior, middle and posterior plane, respectively. MR imaging-based measurements did not differ significantly compared to corresponding cadaveric measurements. The mean values of the lateral/medial tibial capsular reflection on MR imaging were 0.09/0.11 cm, 0.34/0.26 cm and 0.62/0.34 cm in the anterior, middle and posterior plane, respectively. On cadaveric dissection, the maximum mean value was 1.45 cm, measured on the lateral side of the anterior plane. Apart from the lateral aspect of the posterior plane, MR imaging measurements were significantly lower, compared to the corresponding cadaveric measurements. The greatest femoral and tibial capsular reflections were found on the anterior and lateral side of the anterior plane. MR imaging appears to underestimate the distal extent of the knee capsule. Anatomical details of the knee capsule should be considered for safe insertion of external fixator pins.
The Relationship between Financial Development and Energy Consumption in South Africa
Authors: Lefatsa Palesa Milliscent , Sibanda Kin , Garidzirai Rufaro
Published: October 2021

This paper examines the nexus between financial development and energy consumption in South Africa. To determine the long run and short run relationship between financial development and energy consumption in South Africa, the paper uses an Auto Regressive Distributed Lag bounds test (ARDL) and Granger causality test to establish the type of correlation between 1980 and 2018. ARDL bounds testing method offers concrete long-run estimates and t-statistics as it is flexible whether the adopted variables are I(0) or I(1). The study used per capita (kilogram, kg of oil equivalent) to measure total energy consumption, domestic credit to the private sector (percentage of gross domestic product, GDP) to measure financial development, real GDP growth (to capture economic growth), industrial value added (percentage of GDP) to measure industrialization, and urban population (percentage of total population) to capture urbanization. Results from ARDL showed that the relationship between financial development and energy consumption is positive in nature both in short-run and long-run. Granger causality test results revealed unidirectional causality from financial development to energy consumption. Policymakers need to formulate policy reforms that channels more credit to private sector development in order to bolster more energy use in South Africa. There ought to be proper balance between financial development and energy consumption to avoid electricity crisis.
Fabrication of Metal/Carbon Nanotube Composites by Electrochemical Deposition
Authors: Arai Susumu
Published: October 2021

Metal/carbon nanotube (CNT) composites are promising functional materials due to the various superior properties of CNTs in addition to the characteristics of metals, and consequently, many fabrication processes of these composites have been vigorously researched. In this paper, the fabrication process of metal/CNT composites by electrochemical deposition, including electrodeposition and electroless deposition, are comprehensively reviewed. A general introduction for fabrication of metal/CNT composites using the electrochemical deposition is carried out. The fabrication methods can be classified into three types: (1) composite plating by electrodeposition or electroless deposition, (2) metal coating on CNT by electroless deposition, and (3) electrodeposition using CNT templates, such as CNT sheets and CNT yarns. The performances of each type have been compared and explained especially from the view point of preparation methods. In the cases of (1) composite plating and (2) metal coating on CNTs, homogeneous dispersion of CNTs in electrochemical deposition baths is essential for the formation of metal/CNT composites with homogeneous distribution of CNTs, which leads to high performance composites. In the case of (3) electrodeposition using CNT templates, the electrodeposition of metals not only on the surfaces but also interior of the CNT templates is the key process to fabricate high performance metal/CNT composites.
Environmental Influences on Density and Height Growth of Natural Ponderosa Pine Regeneration following Wildfires
Authors: Hammond Darcy H. , Strand Eva K. , Morgan Penelope , Hudak Andrew T. , Newingham Beth A.
Published: October 2021

Over the past century the size and severity of wildfires, as well as post-fire recovery processes (e.g., seedling establishment), have been altered from historical levels due to management policies and changing climate. Tree seedling establishment and growth drive future overstory tree dynamics after wildfire. Post-fire tree regeneration can be highly variable depending on burn severity, pre-fire forest condition, tree regeneration strategies, and climate; however, few studies have examined how different abiotic and biotic factors impact seedling density and growth and the interactions among those factors. We measured seedling density and height growth in the period 2015–2016 on three wildfires that burned in ponderosa pine (Pinus ponderosa) forests in the period 2000–2007 across broad environmental and burn severity gradients. Using a non-parametric multiplicative regression model, we found that downed woody fuel load, duff depth, and fall precipitation best explained variation in seedling density, while the distance to nearest seed tree, a soil productivity index, duff depth, and spring precipitation as snow best explained seedling height growth. Overall, results highlight the importance of burn severity and post-fire climate in tree regeneration, although the primary factors influencing seedling density and height growth vary. Drier conditions and changes to precipitation seasonality have the potential to influence tree establishment, survival, and growth in post-fire environments, which could lead to significant impacts for long-term forest recovery.
Proteomics and Food Analysis: Principles, Techniques, and Applications
Authors: Carrera Mónica
Published: October 2021

Proteomics can be considered the discipline of the large-scale analysis of proteins in a particular biological system [...]
Understanding In Vivo Mastication Behaviour and In Vitro Starch and Protein Digestibility of Pulsed Electric Field-Treated Black Beans after Cooking
Authors: Alpos Marbie , Leong Sze Ying , Liesaputra Veronica , Martin Candace E. , Oey Indrawati
Published: October 2021

The aim of this study was to understand (i) the in vivo mastication behaviour of cooked black beans (chewing duration, texture perception, oral bolus particle size, microstructure, and salivary α-amylase) and (ii) the in vitro digestibility of starch and protein of in vivo-generated black bean oral bolus under simulated gastrointestinal condition. The beans were pre-treated using pulsed electric field (PEF) with and without calcium chloride (CaCl2) addition prior to cooking. The surface response model based on least square was used to optimise PEF processing condition in order to achieve the same texture properties of cooked legumes except for chewiness. In vivo mastication behaviour of the participants (n = 17) was characterized for the particle size of the resulting bolus, their salivary α-amylase activity, and the total chewing duration before the bolus was deemed ready for swallowing. In vitro starch and protein digestibility of the masticated bolus generated in vivo by each participant along the gastrointestinal phase were then studied. This study found two distinct groups of chewers—fast and slow chewers who masticated all black bean beans, on average, for <25 and >29 s, respectively, to achieve a bolus ready for swallowing. Longer durations of chewing resulted in boluses with small-sized particles (majorly composed of a higher number of broken-down cotyledons (2–5 mm2 particle size), fewer seed coats (5–13 mm2 particle size)), and higher activity of α-amylase. Therefore, slow chewers consistently exhibited a higher in vitro digestibility of both the starch and protein of processed black beans compared to fast chewers. Despite such distinct difference in the nutritional implication for both groups of chewers, the in vivo masticated oral bolus generated by fast chewers revealed that the processing conditions involving the PEF and addition of CaCl2 of black beans appeared to significantly (p < 0.05) enhance the in vitro digestibility of protein (by two-fold compared to untreated samples) without stimulating a considerable increase in the starch digestibility. These findings clearly demonstrated that the food structure of cooked black beans created through PEF treatment combined with masticatory action has the potential to modulate a faster hydrolysis of protein during gastrointestinal digestion, thus offering an opportunity to upgrade the quality of legume protein intake in the daily diet.
Varietal Aromas of Fortified Wines from Different Moscato Var. (Vitis vinifera L.) under the Same Pedoclimatic Conditions
Authors: Verzera Antonella , Merlino Maria , Cincotta Fabrizio , Prestia Ottavia , Sparacio Antonio , Sparla Salvatore , Condurso Concetta
Published: October 2021

Vitis vinifera L. cv. Moscato includes different varieties mainly used to produce sweet wines, such as fortified wines. Moscato grapes are characterized by a large number of free and glycosylated monoterpenoids giving very aromatic wines. However, the literature data on the aroma profile of fortified Moscato wines are very limited. In light of this, the present research aimed to investigate the aroma compounds, mainly the varietal ones, of fortified wines from different Moscato varieties, namely Giallo (Yellow), Bianco (White), Bianco at Petit Grain (Blanc à Petits Grains), Ottonel and Rosa (Pink of Trentino), cultivated under the same pedoclimatic conditions. Using the HS-SPME-GC-MS (head space-solid phase micro extraction-gas chromatography-mass spectrometry) technique, numerous varietal and fermentative aroma compounds have been identified and quantified and significant differences were observed among varieties in the levels of mostly volatiles and in their ratios. Based on their composition, the studied wines can be divided in two groups depending on whether linalool or geraniol prevails among varietal aromas. These results are evidence that each Moscato variety has a typical varietal aroma composition, even if some similarities were found between the two white varieties, and between Moscato Giallo and Moscato Ottonel varieties. Moscato Rosa showed a peculiar aroma composition and the lowest ester/terpene ratio.
Development of High-Protein Vegetable Creams by Using Single-Cell Ingredients from Some Microalgae Species
Authors: Boukid Fatma , Comaposada Josep , Ribas-Agustí Albert , Castellari Massimo
Published: October 2021

The aim of this paper was to develop high-protein vegetable creams through the incorporation of microalgae. Single-cell ingredients from Arthrospiraplatensis (spirulina), Chlorella vulgaris, Tetraselmis chui, and Nannochloropsis oceanica were incorporated at two levels of addition (1.5% and 3.0%) to a standard vegetable cream (STD). Effects of incorporation were assessed in terms of physicochemical and rheological attributes as well as nutritional labeling facts. Creams formulated with 3% A. platensis, N. oceanica, or T. chui showed strong color differences (6 < ΔE < 12) compared to STD; creams formulated with 1.5% A. platensis, T. chui, or N. oceanica showed perceptible differences (3 < ΔE < 6); and those made with C. vulgaris at 1.5 and 3% exhibited small differences (ΔE < 2). Moisture content, water activity, pH, syneresis, and °Brix did not show significant changes. Adding microalgae increased Bostwick consistency and decreased the consistency coefficient (K) except in creams made with A. platensis, which showed comparable values to STD. Principal component analysis indicated that creams made with 1.5% C. vulgaris were the most similar to STD considering all evaluated parameters. Estimation of the nutritional labeling facts showed that the four formulations could be labeled as having “high protein content” following the present EU legislation.
Interaction Behavior of Biogenic Material with Electric Arc Furnace Slag
Authors: Huang Xianai , Ng Ka Wing , Giroux Louis , Duchesne Marc , Li Delin , Todoschuk Ted
Published: October 2021

Electric arc furnaces (EAFs) are used for steel production, particularly when recycling scrap material. In EAFs, carbonaceous material is charged with other raw materials or injected into molten slag to generate foam on top of liquid metal to increase energy efficiency. However, the consumption of fossil carbon leads to greenhouse gas emissions (GHGs). To reduce net GHG emissions from EAF steelmaking, the substitution of fossil carbon with sustainable biogenic carbon can be applied. This study explores the possibility of the substitution of fossil material with biogenic material produced by different pyrolysis methods and from various raw materials in EAF steelmaking processes. Experimental work was performed to study the effect of biogenic material utilization on steel and slag composition using an induction melting furnace with 50 kg of steel capacity. The interaction of biogenic material derived from different raw materials and pyrolysis processes with molten synthetic slag was also investigated using a tensiometer. Relative to other biogenic materials tested, a composite produced with densified softwood had higher intensity interfacial reactions with slag, which may be attributed to the rougher surface morphology of the densified biogenic material.
Polymeric Hydrogels for Controlled Release of Black Tea and Coffee Extracts for Topical Applications
Authors: Makhija Pooja , Kathuria Himanshu , Sethi Gautam , Grobben Bert
Published: October 2021

Tea and coffee are popular beverages. Both are also used in topical applications, such as ultraviolet (UV) protection, anti-aging, and wound healing. However, the impact of tea and coffee extract on skin cells is minimally explored. This study investigated the direct exposure of tea and coffee extract on skin cells using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. It was found that direct exposure of tea and coffee to skin cells can be toxic at a high dose on prolonged exposure (72 h). Therefore, it was hypothesized that a formulation providing a controlled release of tea and coffee could improve their skin compatibility. Thermally cross-linked poly(acrylic acid) hydrogels loaded with tea and coffee extracts (with and without milk) were formulated and optimized. The release profiles of these hydrogels were studied at varying loading efficiency. Milk addition with tea extract retarded the tea extract release from hydrogel while minimally affecting the coffee release. This effect was due to the molecular interaction of tea with milk components, showing changes in size, zeta potential, and polydispersity index. The release study best fitted the Korsmeyer–Peppas release model. Skin cells exposed to tea or coffee-loaded hydrogel showed normal skin cell morphology under fluorescence microscopic analysis. In conclusion, the hydrogels controlled the tea and coffee release and showed biocompatibility with skin cells. It can potentially be used for skin applications.
Rock Mass Characterization by UAV and Close-Range Photogrammetry: A Multiscale Approach Applied along the Vallone dell’Elva Road (Italy)
Authors: Migliazza Maria , Carriero Maria Teresa , Lingua Andrea , Pontoglio Emanuele , Scavia Claudio
Published: October 2021

Geostructural rock mass surveys and the collection of data related to discontinues provide the basis for the characterization of rock masses and the study of their stability conditions. This paper describes a multiscale approach that was carried out using both non-contact techniques and traditional support techniques to survey certain geometrical features of discontinuities, such as their orientation, spacing, and useful persistence. This information is useful in identifying the possible kinematics and stability conditions. These techniques are extremely useful in the case study of the Elva valley road (Northern Italy), in which instability phenomena are spread across 9 km in an overhanging rocky mass. A multiscale approach was applied, obtaining digital surface models (DSMs) at three different scales: large-scale DSM of the entire road, a medium-scale DSM to assess portions of the slope, and a small-scale DSM to assess single discontinuities. The georeferenced point cloud and consequent DSMs of the slopes were obtained using an unmanned aerial vehicle (UAV) and terrestrial photogrammetric technique, allowing topographic and rapid traditional geostructural surveys. This technique allowed us to take measurements along the entire road, obtaining geometrical data for the discontinuities that are statistically representative of the rock mass and useful in defining the possible kinematic mechanisms and volumes of potentially detachable blocks. The main purpose of this study was to analyse how the geostructural features of a rock mass can affect the stability slope conditions at different scales in order to identify road sectors susceptible to different potential failure mechanisms using only kinematic analysis.
Do Brief Mindfulness Interventions (BMI) and Health Enhancement Programs (HEP) Improve Sleep in Patients in Hemodialysis with Depression and Anxiety?
Authors: Lavin Paola , Nazar Rim , Nassim Marouane , Noble Helen , Solomonova Elizaveta , Dikaios Elena , Novak Marta , Mucsi Istvan , Trinh Emilie , Potes Angela , Alam Ahsan , Suri Rita S. , Thomas Zoe , Mc Veigh Clare , Lipman Mark , Torres-Platas Susana , Linnaranta Outi , Rej Soham
Published: October 2021

(1) Objective: to determine if a brief mindfulness intervention (BMI) and a health education program (HEP) could improve measures of insomnia in patients undergoing hemodialysis. (2) Methods: this was a planned secondary analysis of a randomized controlled trial of BMI vs. HEP for hemodialysis patients with depression and/or anxiety symptoms. The primary outcome for the analysis was the Athens Insomnia Scale (AIS). The secondary outcome was consolidation of daily inactivity (ConDI), an actigraphy measure that describes sleep continuity and is based on a sleep detection algorithm validated by polysomnography. We also explored whether changes in AIS and ConDI were associated with changes in depression, anxiety, and quality of life scores over 8-week follow-up. (3) Results: BMI and HEP groups did not differ significantly from one another. Exposure to BMI or HEP improved sleep quality (baseline AIS 9.9 (±5.0) vs. 8-week follow-up 6.4 (±3.9), (V = 155.5, p = 0.015)), but not ConDI. Improvements in AIS were associated with lower depression scores (Rho = 0.57, p = 0.01) and higher quality-of-life scores (Rho = 0.46, p = 0.04). (4) Conclusions: mindfulness and HEP may be helpful interventions to improve self-reported sleep quality in patients undergoing hemodialysis. Decreases in insomnia scores were associated with decreased depression symptoms and increased quality of life scores.
Cost and Effectiveness of Long-Term Care Following Integrated Discharge Planning: A Prospective Cohort Study
Authors: Wang Yu-Chun , Lee Wen-Ying , Chou Ming-Yueh , Liang Chih-Kuang , Chen Hsueh-Fen , Yeh Shu-Chuan Jennifer , Yaung Chih-Liang , Tsai Kang-Ting , Huang Joh-Jong , Wang Chi , Lin Yu-Te , Lou Shi-Jer , Shi Hon-Yi
Published: October 2021

Little is known about the effects of seamless hospital discharge planning on long-term care (LTC) costs and effectiveness. This study evaluates the cost and effectiveness of the recently implemented policy from hospital to LTC between patients discharged under seamless transition and standard transition. A total of 49 elderly patients in the standard transition cohort and 119 in the seamless transition cohort were recruited from November 2016 to February 2018. Data collected from medical records included the Multimorbidity Frailty Index, Activities of Daily Living Scale, and Malnutrition Universal Screening Tool during hospitalization. Multiple linear regression and Cox regression models were used to explore risk factors for medical resource utilization and medical outcomes. After adjustment for effective predictors, the seamless cohort had lower direct medical costs, a shorter length of stay, a higher survival rate, and a lower unplanned readmission rate compared to the standard cohort. However, only mean total direct medical costs during hospitalization and 6 months after discharge were significantly (p < 0.001) lower in the seamless cohort (USD 6192) compared to the standard cohort (USD 8361). Additionally, the annual per-patient economic burden in the seamless cohort approximated USD 2.9–3.3 billion. Analysis of the economic burden of disability in the elderly population in Taiwan indicates that seamless transition planning can save approximately USD 3 billion in annual healthcare costs. Implementing this policy would achieve continuous improvement in LTC quality and reduce the financial burden of healthcare on the Taiwanese government.
Comparison of Motor Vehicle Collision Injuries between Pregnant and Non-Pregnant Women: A Nationwide Collision Data-Based Study
Authors: Koh Soonho , Hitosugi Masahito , Moriguchi Shingo , Baba Mineko , Tsujimura Seiji , Takeda Arisa , Takaso Marin , Nakamura Mami
Published: October 2021

We compared the independent predictive factors for moderate and severe injuries, along with characteristics and outcomes of motor vehicle collisions, between pregnant and non-pregnant women. Using 2001–2015 records from the National Automotive Sampling System/Crashworthiness Data System, we selected 736 pregnant women and 21,874 non-pregnant women having any anatomical injuries. Pregnant women showed less severe collisions, fewer fatalities, and less severe injuries in most body regions than non-pregnant women. In pregnant women, the rate of sustaining abbreviated injury scale (AIS) scores 2+ injuries was higher for the abdomen only. For non-pregnant women, rear seat position, airbag deployment, multiple collisions, rollover, force from the left, and higher collision velocity had a positive influence on the likelihood of AIS 2+ injuries, and seatbelt use and force from the rear had a negative influence. There is a need for further development of passive safety technologies for restraint and active safety features to slow down vehicles and mitigate collisions. The influencing factors identified may be improved by safety education. Therefore, simple and effective interventions by health professionals are required that are tailored to pregnant women.
Current Status of CAR-T Cell Therapy in Multiple Myeloma
Authors: Reguera-Ortega Juan Luis , García-Guerrero Estefanía , Pérez-Simón Jose Antonio
Published: October 2021

Current data on CAR-T cell-based therapy is really promising in multiple myeloma, especially in terms of response. In heavily pretreated patients, who have already received proteasome inhibitors, immunomodulatory drugs and monoclonal antibodies, current trials report an overall response rate ranging from 81 to 97% and 45 to 67% of complete remission rates. Data are less encouraging in terms of duration of response, although most recent trials have shown significant improvements in terms of event-free survival, with medians ranging from 8 to 14 months and up to 77% progression-free survival at 12 months with an acceptable toxicity profile. These data will be consolidated in future years and will provide new evidence on the best timing for CAR-T cell therapy. Moreover, new CAR-T designs are underway and will challenge the current results.
Influence of Ripening on Polyphenolic Content, Degradative, and Browning Enzymes in Cantaloupe Varieties (C. Melo, L.)
Authors: Vella Filomena Monica , Calandrelli Roberto , Laratta Bruna
Published: October 2021

The biochemical changes that occur during the growth and ripening of fruit and vegetable tissues, especially for color and firmness, are the most important factors affecting the quality of fresh products. Cantaloupe (Cucumis melo, L.) is one of the main economically important fruits in the world and its quality parameters, e.g., sweetness, nutritional factors, and texture, influence consumer preferences. Hence, these two features, appearance and texture changes, were investigated in three different genotypes of netted melon, all characterized by an extended shelf life but with different ripening phases. In particular, in all melon cultivars, the cell wall-modifying enzymatic activities and indicators of softening as well as total polyphenols, ortho-diphenols, flavonoids, and tannins, and antioxidant activity were studied. One variety with excellent shelf-life displayed the best nutritional and healthy qualities, in the early stages of ripening, and the lowest degree of browning. The lytic enzyme activities were reduced in the initial stages and after they increased gradually until the overripe stage, with the same trend for all varieties under investigation. The antioxidant activities declined with increasing time of ripeness in all genotypes. The outcomes confirm that the activities of both classes examined, antioxidant and cell wall-modifying enzymes, may vary significantly during ripeness depending on the genotype, suggesting the involvement in determining the postharvest behavior of these fruits.
Analysis of Light-Independent Anthocyanin Accumulation in Mango (Mangifera indica L.)
Authors: Shi Bin , Wu Hongxia , Zheng Bin , Qian Minjie , Gao Aiping , Zhou Kaibing
Published: October 2021

Light dependent anthocyanin accumulation contributes to the red pigmentation of the fruit skin of mango (Mangifera indica L.). Light-independent pigmentation has also been reported, but remains poorly characterized. In this study, the pigmentation patterns in the skin of two red mango cultivars, ‘Ruby’ and ‘Sensation’, were evaluated. Metabolomic profiling revealed that quercetin-3-O-glucoside, cyanidin-3-O-galactoside, procyanidin B1, and procyanidin B3 are the predominant flavonoid compounds in the skin of ‘Ruby’ and ‘Sensation’ fruit. Young fruit skin mainly accumulates flavonol and proanthocyanidin, while anthocyanin is mainly accumulated in the skin of mature fruit. Bagging treatment inhibited the biosynthesis of flovonol and anthocyanin, but promoted the accumulation of proanthocyanidin. Compared with ‘Sensation’, matured ‘Ruby’ fruit skin showed light red pigmentation at 120 days after full bloom (DAFB), showing a light-independent anthocyanin accumulation pattern. However, the increase of anthocyanin concentration, and the expression of key anthocyanin structural and regulatory genes MiUFGT1, MiUFGT3, and MiMYB1 in the skin of bagged ‘Ruby’ fruit versus ‘Sensation’ at 120 DAFB was very limited. There was no mutation in the crucial elements of MiMYB1 promoter between ‘Ruby’ and ‘Sensation’. We hypothesize that the light-independent anthocyanin accumulation in the skin of mature ‘Ruby’ fruit is regulated by plant hormones, and that ‘Ruby’ can be used for breeding of new more easily pigmented red mango cultivars.
Chemical Composition and Anti-Microbial Activity of Hog Plum (Spondias mombin L.) Peel Oil Extracted from Different Regions of Tropical Climates
Authors: Plabon Md. Emrul Ahsan , Mondal Shakti Chandra , Or Rashid Md. Mamun , Chowdhury M. Kaium , Saeid Abu , Althobaiti Fayez , Dessok Eldessoky S. , Rehmani Muhammad Ishaq Asif , Mustafa Syed Khalid , Islam Mohammad Sohidul
Published: October 2021

The hydro-distilled essential oil of hog plum peel may be used for enhancing the flavor and taste of food products as well as for hiding the unpleasant odor of drugs. Thus, the waste peels of Spondias mombin appear to have economic importance. To find out the chemical composition and anti-microbial properties of hog plum peel oil, the samples were collected from different regions of Bangladesh for extraction and identification of volatile compounds by GC-MS, where dichloromethane was used as an extraction solvent. The required standard analytical methods were used to assay the anti-microbial properties of hog plums. In this study, pentenyl-3-thy-met-4-alpha-methyl-alpha-ethanol-oxiranen (29.04%), (3,3.1,1)-4-dimethylethyl-1,1-phenol (8.00%), cycohexanol-3 (10.85%), 4-hydroxy-penzeneethanamine (7.09%), hydroxylamine (4.63%), dibutyl phthalate (6.85%), etc., were majorly determined. Consequently, the highest content of 75.81% volatile compounds was found in the Dinajpur district, where the lowest content of 35.00% was found in the Rajshahi district. In contrast, 33 volatile compounds were identified in hog plum peels collected from the Barishal district, whereas 22 compounds were detected in the peel samples collected from the Dinajpur district. In addition, the antimicrobial activity of the oil was analyzed by the disk diffusion method, and the results revealed that the highest Ciprocin content was recorded in the hog plums of Barishal (22.0–23.0 mm), while the lowest was recorded in the Mymensingh sample (20.67–21.63 mm), which was on par with Rajshahi sample (20.70–21.50 mm). The results of the anti-fungal activities of the peel oil showed the highest zone of inhibition against the Aspergillus niger (11.63 ± 0.0003 mm) and Penicillium oxalicum (13.67 ± 1.97 mm) content of the Rajshahi and Pabna district samples, respectively.
Improving Customisation in Clinical Pathways by Using a Modular Perspective
Authors: van Dam Anne , Metz Margot , Meijboom Bert
Published: October 2021

A standardised system of clinical pathways often conflicts with providing patient-centred heterogeneous care. Mental health care organisations are searching for new methods to become responsive towards unique treatment needs. Modularity is a method increasingly suggested to reconcile standardisation and customisation. The aim is to investigate the extent to which modularity can be applied to make clinical pathways in specialist mental health care more flexible in order to stimulate shared decision making (SDM) and thereby customise care processes to patient contexts while maintaining evidence-based standards. Methods consist of literature research and a theory-based case study including document analysis and semi-structured interviews, which were performed at a Dutch specialist mental health care organisation. The results show that in current literature two modularity-based structures are proposed that support flexibility and customisation, i.e., ‘Prototype’ and ‘Menu-based’. This study reveals that departments tend to use the prototype method if they have predictable patient needs, evidence-based methods are available and there is sequency in treatment components. The menu-based method is preferred if there are unpredictable needs, or the evidence needed to create interconnectedness in treatment is lacking. In conclusion, prototype or menu-based methods are both suitable for applying SDM and reaching customisation in practice. The choice is determined by three characteristics: predictability of needs, availability of evidence and the interconnectedness of treatment components.