bluuebunny/crossref_metadata_2025 huggingface dataset:
Linguistic adaptation and psychometric evaluation of Italian version of children’s sleep habits questionnaire
Authors: Borrelli Melissa , Scala Iris , Festa Paola , Bruzzese Dario , Michelotti Ambrosina , Cantone Elena , Corcione Adele , Fragnito Martina , Miranda Vincenzo , Santamaria Francesca
Published: August 2021

Background

The Children’s Sleep Habits Questionnaire (CSHQ) is a parent-report questionnaire used to examine sleep behavior in children. Linguistic adaptation of CSHQ into several languages and/or psychometric analysis of reliability have been published.

Main text

Our aim was to translate the original 33-items CSHQ from English to Italian and to examine its reliability for use in 4–10 years-old children of Italy. After translation and back-translation procedure of the original CSHQ, the Italian CSHQ (CSHQ-IT) was administered to 69 mothers of healthy children. Reliability of CSHQ-IT was examined by the internal consistency of the scale (using the Cronbach’s alpha coefficient), and by the test-retest analysis obtained by asking mothers who had completed the CSHQ-IT at baseline to re-complete it after a two-week interval (measured with the Lin’s Concordance Correlation Coefficient, CCC). The CSHQ-IT showed adequate internal consistency (Cronbach’s alpha = 0.81 for the total scale). The total CSHQ-IT score showed a strong correlation in retests (CCC 0.87; 95% Confidence Interval, 0.80; 0.92).

Conclusion

CSHQ-IT is a valuable tool for evaluating sleep behavior in children 4–10 years-old in Italy. Future research should be implemented to provide definitive validity of CSHQ-IT in children with sleep-disordered breathing.
Management of severe trauma worldwide: implementation of trauma systems in emerging countries: China, Russia and South Africa
Authors: Zhou Jing , Wang Tianbing , Belenkiy Igor , Hardcastle Timothy Craig , Rouby Jean-Jacques , Jiang Baoguo ,   for the International Trauma Rescue & Treatment Association (ITRTA) Study Group, Demetriades Demetrios , Oestern Hans J. , Iwase Hiroaki , Zhang Mao , Bouzat Pierre , Coats Timothy J. , Gauss Tobias , An Youzhong
Published: August 2021

As emerging countries, China, Russia, and South Africa are establishing and/or improving their trauma systems. China has recently established a trauma system named “the Chinese Regional Trauma Care System” and covered over 200 million populations. It includes paramedic-staffed pre-hospital care, in-hospital care in certified trauma centers, trauma registry, quality assurance, continuous improvement and ongoing coverage of the entire Chinese territory. The Russian trauma system was formed in the first decade of the twenty-first century. Pre-hospital care is region-based, with a regional coordination center that determines which team will go to the scene and the nearest hospital where the victim should be transported. Physician-staffed ambulances are organized according to three levels of trauma severity corresponding to three levels of trauma centers where in-hospital care is managed by a trauma team. No national trauma registry exists in Russia. Improvements to the Russian trauma system have been scheduled. There is no unified trauma system in South Africa, and trauma care is organized by public and private emergency medical service in each province. During the pre-hospital care, paramedics provide basic or advanced life support services and transport the patients to the nearest hospital because of the limited number of trauma centers. In-hospital care is inclusive with a limited number of accredited trauma centers. In-hospital care is managed by emergency medicine with multidisciplinary care by the various specialties. There is no national trauma registry in South Africa. The South African trauma system is facing multiple challenges. An increase in financial support, training for primary emergency trauma care, and coordination of private sector, need to be planned.
The identification and semi-quantitative assessment of gastrointestinal nematodes in faecal samples using multiplex real-time PCR assays
Authors: Reslova Nikol , Skorpikova Lucie , Kyrianova Iveta Angela , Vadlejch Jaroslav , Höglund Johan , Skuce Philip , Kasny Martin
Published: August 2021

Background

The diagnosis of gastrointestinal nematode (GIN) infections in ruminants is routinely based on morphological/morphometric analysis of parasite specimens recovered by coprological methods, followed by larval culture (LC) techniques. Such an approach is laborious, time-consuming, requires a skilled expert, and moreover suffers from certain limitations. Molecular tools are able to overcome the majority of these issues, providing accurate identification of nematode species and, therefore, may be valuable in sustainable parasite control strategies.

Methods

Two multiplex real-time polymerase chain reaction (PCR) assays for specific detection of five main and one invasive GIN species, including an internal amplification control to avoid false-negative results, were designed targeting SSU rRNA and COI genetic markers, as well as established ITS1/2 sequences. The assays were optimized for analysis of DNA extracted directly from sheep faeces and verified for Haemonchus contortus, Teladorsagia circumcincta, Trichostrongylus colubriformis, Nematodirus battus, Chabertia ovina, and Ashworthius sidemi. Semi-quantitative evaluation of infection intensity was enabled using a plasmid construct and a dilution series of sheep faeces with a known number of nematode eggs. Assays were tested on 44 individually collected faecal samples from three farms, and results were compared to those from faecal egg counts (FEC) using the concentration McMaster technique and LC.

Results

Multiplex real-time PCR assays showed great specificity to target nematodes. During the analysis of faecal samples, the assays proved to have higher sensitivity in strongylid-type egg detection over FEC by revealing three false-negative samples, while showing moderate agreement in evaluation of infection intensity. The multiplex assays further clarified GIN species identification compared to LC, which had confused determination of Teladorsagia spp. for Trichostrongylus spp.

Conclusions

Our multiplex assays proved to be a rapid and accurate approach enabling simultaneous and reliable GIN species identification from faeces and semi-quantitative estimation of the number of eggs present. This approach increases diagnostic value and may add a high degree of precision to evaluation of anthelmintic efficacy, where it is important to identify species surviving after treatment.

Graphical Abstract
A nationwide survey of the tabanid fauna of Cameroon
Authors: Sevidzem Silas L. , Koumba Aubin A. , Yao-Acapovi Genevieve L. , Mavoungou Jacques F.
Published: August 2021

Background

Tabanids are a neglected group of haematophagous dipterans despite containing 4434 species, regrouped in > 144 genera. They are mechanical vectors of important pathogens, including viruses, bacteria and protozoa of humans and domesticated and wild animals. As it is > 50 years since the publication of a preliminary nationwide record of the tabanids of Cameroon identified 84 species, updated information is needed. The aim of this study was to provide current data on the species composition, abundance and distribution of tabanids in the five main agro-ecological zones (AEZs) of Cameroon.

Methods

From 2015 to 2017, a systematic entomological study using Nzi, Vavoua, Biconical and Sevi traps (n = 106) was conducted in 604 trapping points over 11,448 trap-days in the five main AEZs of Cameroon.

Results

A total of 25,280 tabanids belonging to 25 species were collected, including eight species not previously documented in Cameroon, namelyTabanus latipes(1 female),Tabanus ricardae(1 female),Tabanus fasciatus(32 females and 6 males),Haematopota pluvialis(18 females),Haematopota decora(19 females and 3 males),Haematopota nigripennis(18 females),Chrysops distinctipennis(47 females and 5 males) andAncala fasciata(34 females and 7 males). The distribution maps of the newly identified tabanids differed between AEZs, with most tabanids collected from the Guinean savanna. The highest apparent density of tabanids was recorded in the Sudan Savanna region, and the mean apparent densities of species with sites was statistically significantly different (Student t-test: 2.519,df = 24,P = 0.019). The highest species diversity was found in the rainforest.

Conclusions

This study increased the list of tabanids recorded in Cameroon from 84 species in the preliminary record to 92 species, with most of the newly identified species occurring in the Guinea Savanna AEZ. The high diversity and abundance of tabanids in the livestock/wildlife interface areas of the rain forests and Sudan Savanna AEZs, respectively, suggest risk of mechanical transmission of pathogens. Investigations of the microbiota of tabanids in the different AEZs to define their role as disease vectors are proposed.

Graphical abstract
Multiple diagnostic tests demonstrate an increased risk of canine heartworm disease in northern Queensland, Australia
Authors: Panetta Jessica L. , Calvani Nichola Eliza Davies , Orr Bronwyn , Nicoletti Aldo Gianfranco , Ward Michael P. , Šlapeta Jan
Published: August 2021

Background

Canine heartworm (Dirofilaria immitis) is a life-threatening infection of dogs with a global distribution. Information on the prevalence of D. immitis and associated risk factors for canine heartworm antigen positivity—and thus disease—in Australia is scarce or outdated. The current reference method for D. immitis diagnosis in dogs is via the detection of heartworm antigen in blood using commercially available microwell-based enzyme-linked immunosorbent assays (ELISAs). Heat treatment of canine plasma prior to testing has been suggested to increase test sensitivity. The aim of the current study was to estimate the prevalence of D. immitis in dogs confined to shelters in Queensland, Australia. The impact of heat treatment on antigen test results was also assessed.

Methods

Blood samples (n = 166) were collected directly from dogs in seven shelters across Queensland (latitudinal span of approx. 1700 km) into EDTA blood collection tubes. A commercially available ELISA (DiroCHEK®) was used to detect canine heartworm antigen in untreated and heat-treated plasma. Whole blood was concurrently tested for the presence of microfilariae and D. immitis DNA using a modified Knott’s test and real-time PCR, respectively. Risk factors (age, gender, source, location) associated with the odds of positivity for canine heartworm were assessed using binary logistic regression models.

Results

A total of 16 dogs (9.6%; 95% confidence interval [CI]: 5.9–15.2%) were positive for canine heartworm based on combined test results. Heat treatment did not impact on the positivity of D. immitis antigen within samples (Cohen’s kappa = 0.98), but the optical density was significantly increased in paired plasma samples for D. immitis antigen-positive samples (Wilcoxon matched-pairs signed rank test, two-tailed P < 0.01). Location of the dog in a shelter in northern Queensland was the only risk factor significantly associated with the odds of a dog being more likely to be D. immitis antigen positive (odds ratio: 4.39; 95% CI: 1.26–13.51). All samples positive for the modified Knott’s test were also positive for D. immitis DNA by PCR.

Conclusions

This study demonstrated the presence of heartworm-positive dogs in shelters in Queensland, with positive animals significantly more likely to occur in northern Queensland than southern Queensland. Sustained testing for the presence of D. immitis microfilariae and antigen remain important diagnostic tools in areas with known and re-emerging canine heartworm activity.

Graphical Abstract
The DNA methylation landscape of multiple myeloma shows extensive inter- and intrapatient heterogeneity that fuels transcriptomic variability
Authors: Derrien Jennifer , Guérin-Charbonnel Catherine , Gaborit Victor , Campion Loïc , Devic Magali , Douillard Elise , Roi Nathalie , Avet-Loiseau Hervé , Decaux Olivier , Facon Thierry , Mallm Jan-Philipp , Eils Roland , Munshi Nikhil C. , Moreau Philippe , Herrmann Carl , Magrangeas Florence , Minvielle Stéphane
Published: August 2021

Background

Cancer evolution depends on epigenetic and genetic diversity. Historically, in multiple myeloma (MM), subclonal diversity and tumor evolution have been investigated mostly from a genetic perspective.

Methods

Here, we performed an analysis of 42 MM samples from 21 patients by using enhanced reduced representation bisulfite sequencing (eRRBS). We combined several metrics of epigenetic heterogeneity to analyze DNA methylation heterogeneity in MM patients.

Results

We show that MM is characterized by the continuous accumulation of stochastic methylation at the promoters of development-related genes. High combinatorial entropy change is associated with poor outcomes in our pilot study and depends predominantly on partially methylated domains (PMDs). These PMDs, which represent the major source of inter- and intrapatient DNA methylation heterogeneity in MM, are linked to other key epigenetic aberrations, such as CpG island (CGI)/transcription start site (TSS) hypermethylation and H3K27me3 redistribution as well as 3D organization alterations. In addition, transcriptome analysis revealed that intratumor methylation heterogeneity was associated with low-level expression and high variability.

Conclusions

We propose that disrupted DNA methylation in MM is responsible for high epigenetic and transcriptomic instability allowing tumor cells to adapt to environmental changes by tapping into a pool of evolutionary trajectories.
Decontamination, pooling and dereplication of the 678 samples of the Marine Microbial Eukaryote Transcriptome Sequencing Project
Authors: Van Vlierberghe Mick , Di Franco Arnaud , Philippe Hervé , Baurain Denis
Published: August 2021

Objectives

Complex algae are photosynthetic organisms resulting from eukaryote-to-eukaryote endosymbiotic-like interactions. Yet the specific lineages and mechanisms are still under debate. That is why large scale phylogenomic studies are needed. Whereas available proteomes provide a limited diversity of complex algae, MMETSP (Marine Microbial Eukaryote Transcriptome Sequencing Project) transcriptomes represent a valuable resource for phylogenomic analyses, owing to their broad and rich taxonomic sampling, especially of photosynthetic species. Unfortunately, this sampling is unbalanced and sometimes highly redundant. Moreover, we observed contaminated sequences in some samples. In such a context, tree inference and readability are impaired. Consequently, the aim of the data processing reported here is to release a unique set of clean and non-redundant transcriptomes produced through an original protocol featuring decontamination, pooling and dereplication steps.

Data description

We submitted 678 MMETSP re-assembly samples to our parallel consolidation pipeline. Hence, we combined 423 samples into 110 consolidated transcriptomes, after the systematic removal of the most contaminated samples (186). This approach resulted in a total of 224 high-quality transcriptomes, easy to use and suitable to compute less contaminated, less redundant and more balanced phylogenies.
PRC2: an epigenetic multiprotein complex with a key role in the development of rhabdomyosarcoma carcinogenesis
Authors: Zoroddu Stefano , Marchesi Irene , Bagella Luigi
Published: August 2021

Skeletal muscle formation represents a complex of highly organized and specialized systems that are still not fully understood. Epigenetic systems underline embryonic development, maintenance of stemness, and progression of differentiation. Polycomb group proteins play the role of gene silencing of stemness markers that regulate muscle differentiation. Enhancer of Zeste EZH2 is the catalytic subunit of the complex that is able to trimethylate lysine 27 of histone H3 and induce silencing of the involved genes. In embryonal Rhabdomyosarcoma and several other tumors, EZH2 is often deregulated and, in some cases, is associated with tumor malignancy. This review explores the molecular processes underlying the failure of muscle differentiation with a focus on the PRC2 complex. These considerations could open new studies aimed at the development of new cutting-edge therapeutic strategies in the onset of Rhabdomyosarcoma.
Evaluating QualiCO: an ontology to facilitate qualitative methods sharing to support open science
Authors: Hocker Julian , Bipat Taryn , McDonald David W. , Zachry Mark
Published: August 2021

Qualitative science methods have largely been omitted from discussions of open science. Platforms focused on qualitative science that support open science data and method sharing are rare. Sharing and exchanging coding schemas has great potential for supporting traceability in qualitative research as well as for facilitating the reuse of coding schemas. In this study, we present and evaluate QualiCO, an ontology to describe qualitative coding schemas. Twenty qualitative researchers used QualiCO to complete two coding tasks. In our findings, we present task performance and interview data that focus participants’ attention on the ontology. Participants used QualiCO to complete the coding tasks, decreasing time on task, while improving accuracy, signifying that QualiCO enabled the reuse of qualitative coding schemas. Our discussion elaborates some issues that participants had and highlights how conceptual and prior practice frames their interpretation of how QualiCO can be used.
MRI-detected extramural venous invasion of rectal cancer: Multimodality performance and implications at baseline imaging and after neoadjuvant therapy
Authors: Inoue Akitoshi , Sheedy Shannon P. , Heiken Jay P. , Mohammadinejad Payam , Graham Rondell P. , Lee Hee Eun , Kelley Scott R. , Hansel Stephanie L. , Bruining David H. , Fidler Jeff L. , Fletcher Joel G.
Published: August 2021

MRI is routinely used for rectal cancer staging to evaluate tumor extent and to inform decision-making regarding surgical planning and the need for neoadjuvant and adjuvant therapy. Extramural venous invasion (EMVI), which is intravenous tumor extension beyond the rectal wall on histopathology, is a predictor for worse prognosis. T2-weighted images (T2WI) demonstrate EMVI as a nodular-, bead-, or worm-shaped structure of intermediate T2 signal with irregular margins that arises from the primary tumor. Correlative diffusion-weighted images demonstrate intermediate to high signal corresponding to EMVI, and contrast enhanced T1-weighted images demonstrate tumor signal intensity in or around vessels. Diffusion-weighted and post contrast images may increase diagnostic performance but decrease inter-observer agreement. CT may also demonstrate obvious EMVI and is potentially useful in patients with a contraindication for MRI. This article aims to review the spectrum of imaging findings of EMVI of rectal cancer on MRI and CT, to summarize the diagnostic accuracy and inter-observer agreement of imaging modalities for its presence, to review other rectal neoplasms that may cause EMVI, and to discuss the clinical significance and role of MRI-detected EMVI in staging and restaging clinical scenarios.
RETRACTED ARTICLE: Using a new HSPC senescence model in vitro to explore the mechanism of cellular memory in aging HSPCs
Authors: Dong Yongpin , Guo Chunni , Zhou Wuxiong , Li Wenfang , Zhang Lina
Published: August 2021

Background

Age-associated changes attenuate human blood system functionality through the aging of hematopoietic stem and progenitor cells (HSPCs), manifested in human populations an increase in myeloproliferative disease and even leukemia; therefore, study on HSPC senescence bears great significance to treat hematopoietic-associated disease. Furthermore, the mechanism of HSPC aging is lacking, especially the cellular memory mechanism. Here, we not only reported a new HSPC senescence model in vitro, but also propose and verify the cellular memory mechanism of HSPC aging of the Polycomb/Trithorax system.

Methods

HSPCs (Lin−c-kit+ cells) were isolated and purified by magnetic cell sorting (MACS). The proportions and cell cycle distribution of cells were determined by flow cytometry; senescence-related β-galactosidase assay, transmission electron microscope (TEM), and colony-forming unit (CFU)-mix assay were detected for identification of the old HSPC model. Proteomic tests and RNA-seq were applied to analyze differential pathways and genes in the model cells. qPCR, Western blot (WB), and chromatin immunoprecipitation PCR (CHIP-PCR) were used to detect the gene expression of cell memory-related proteins. Knockdown of cell memory-related key genes was performed with shRNA interference.

Results

In the model old HSPCs, β-gal activity, cell cycle, colony-forming ability, aging-related cell morphology, and metabolic pathway were significantly changed compared to the young HSPCs. Furthermore, we found the model HSPCs have more obvious aging manifestations than those of natural mice, and IL3 is the major factor contributing to HSPC aging in the model. We also observed dramatic changes in the expression level of PRC/TrxG complexes. After further exploring the downstream molecules of PRC/TrxG complexes, we found that Uhrf1 and TopII played critical roles in HSPC aging based on the HSPC senescence model.

Conclusions

These findings proposed a new HSPC senescence model in vitro which we forecasted could be used to preliminary screen the drugs of the HSPC aging-related hemopathy and suggested cellular memory mechanism of HSPC aging.
Imaging and modelling of poly(3-hydroxybutyrate) synthesis in Paracoccus denitrificans
Authors: Bordel Sergio , van Spanning Rob J. M. , Santos-Beneit Fernando
Published: August 2021

Poly(3-hydroxybutyrate) (PHB) granule formation in Paracoccus denitrificans Pd1222 was investigated by laser scanning confocal microscopy (LSCM) and gas chromatography analysis. Cells that had been starved for 2 days were free of PHB granules but resynthesized them within 30 min of growth in fresh medium with succinate. In most cases, the granules were distributed randomly, although in some cases they appeared in a more organized pattern. The rates of growth and PHB accumulation were analyzed within the frame of a Genome-Scale Metabolic Model (GSMM) containing 781 metabolic genes, 1403 reactions and 1503 metabolites. The model was used to obtain quantitative predictions of biomass yields and PHB synthesis during aerobic growth on succinate as sole carbon and energy sources. The results revealed an initial fast stage of PHB accumulation, during which all of the acetyl-CoA originating from succinate was diverted to PHB production. The next stage was characterized by a tenfold lower PHB production rate and the simultaneous onset of exponential growth, during which acetyl-CoA was predominantly drained into the TCA cycle. Previous research has shown that PHB accumulation correlates with cytosolic acetyl-CoA concentration. It has also been shown that PHB accumulation is not transcriptionally regulated. Our results are consistent with the mentioned findings and suggest that, in absence of cell growth, most of the cellular acetyl-CoA is channeled to PHB synthesis, while during exponential growth, it is drained to the TCA cycle, causing a reduction of the cytosolic acetyl-CoA pool and a concomitant decrease of the synthesis of acetoacetyl-CoA (the precursor of PHB synthesis).
Interoperability technology of sports health monitoring equipment based on multi-sensor information fusion
Authors: Zhang Di
Published: August 2021

Nowadays, the pace of society is accelerating, people’s living standard has been greatly improved compared with the past, and we pay more and more attention to the real-time, flexibility, and intelligence of medical services. Information fusion technology is the study of how to effectively and comprehensively utilize multi-sensor information to overcome information imperfections and uncertainties. In recent years, it has become a hotspot for research, but with the development of communication technology, electronic technology, and computers, the form of information becomes complicated and diverse in science and technology such as technology. In this case, the information obtained by a single sensor is always imperfect, so multiple sensors must be used to detect the target from multiple angles. Obtaining more dimensional information about the target and fusing it to ensure the integrity and accuracy of the information helps to accurately determine the physiological health of the human body. This paper analyzes the multi-sensor information fusion technology in detail, discusses the basic principle of multi-sensor information fusion, fusion process, fusion level, architecture and general fusion algorithm, constructs a sensor information fusion technology and its application model in biomedical detection, from the application framework, control structure of multi-sensor integrated system, information fusion, etc. The current situation of multi-sensor information fusion technology is studied in several aspects. An interoperability technology of sports health monitoring equipment based on multi-sensor information fusion is constructed. The experimental results show that the experimental efficiency is improved by 20% after using the application model in this paper, which has certain practicability.
Effect of carcass contamination on necrophagous invertebrate performance
Authors: Hunting Ellard R. , Schrama Maarten , Rozen Daniel E. , Joenssen Christina , Brun Nadja R.
Published: August 2021

Background

The breakdown of dead organic matter is driven by a diverse array of organisms and is an important process increasingly impacted by a range of contaminants. While many studies have documented how contaminants affect food webs that are fueled by decaying plant litters, much less is known about how contaminants affect organisms that rely on dead animal material. Here, we begin to explore the effects of food contamination—using silver nanoparticles (AgNPs) as a model contaminant—on the carrion beetle Nicrophorus vespilloides that buries carcasses of small vertebrates in soils as food source and larval nursing grounds.

Results

Our data show that a single ingestion of a non-lethal dose of 1 μg mL−1 AgNPs by adult female beetles does not affect overall gut microbial activity but results in shifts in the gut microbial community composition towards pathogens including Alcaligenes, Morganella, and Pseudomonas. While no effects were observed in offspring clutch size, some reductions were visible in clutch weight, number of larvae, and number of eclosing pupae in exposed N. vespilloides in comparison with controls. Repeated ingestion of AgNPs over several weeks led to a decrease in survival of adult beetles, suggesting that more environmentally realistic exposure scenarios can directly affect the success of carcass-feeding animals.

Conclusions

Sub-lethal carcass contamination with a model pollutant can affect the gut microbial composition in female beetles and reduce offspring fitness. This encourages consideration of currently overlooked propagation routes of contaminants through necrophagous food webs and inherent consequences for ecological and evolutionary processes.
Optimization of cerebral organoids: a more qualified model for Alzheimer’s disease research
Authors: Bi Feng-Chen , Yang Xin-He , Cheng Xiao-Yu , Deng Wen-Bin , Guo Xiao-Li , Yang Hui , Wang Yin , Li Juan , Yao Yao
Published: August 2021

Alzheimer’s disease (AD) is a neurodegenerative disease that currently cannot be cured by any drug or intervention, due to its complicated pathogenesis. Current animal and cellular models of AD are unable to meet research needs for AD. However, recent three-dimensional (3D) cerebral organoid models derived from human stem cells have provided a new tool to study molecular mechanisms and pharmaceutical developments of AD. In this review, we discuss the advantages and key limitations of the AD cerebral organoid system in comparison to the commonly used AD models, and propose possible solutions, in order to improve their application in AD research. Ethical concerns associated with human cerebral organoids are also discussed. We also summarize future directions of studies that will improve the cerebral organoid system to better model the pathological events observed in AD brains.
Farmer-herder conflicts, tenure insecurity and farmer’s investment decisions in Agogo, Ghana
Authors: Kugbega Selorm Kobla , Aboagye Prince Young
Published: August 2021

Owing to climate change, population growth and tenurial changes, the past decade has witnessed high interest among migrant and settler pastoralist groups in the vegetal-rich customary lands of the Agogo Traditional Area. This has resulted in lease grants of large land areas to pastoralists by traditional authorities and usufruct families, for reasons of ensuring optimum use and gaining the highest returns from lands. This paper examines the implications of consequent competing interests over land resources between farmers and herders on indigenous farmer’s agricultural investment decisions. The study uses qualitative methods and empirical evidence is given by primary data from semi-structured interviews and focus group discussions in the case study area. Results indicated that land owners exploit lapses in customary land administration systems to allocate lands in exchange for money, to pastoralists while neglecting indigenous farmers’ land use rights. Thus, indigenous farmers report land tenure insecurity and a sense of deprivation from their customary lands. Despite tenure insecurity concerns, farmer’s agricultural investment decisions remain unchanged because such changes in investment decisions may reduce incomes and compromise their livelihoods. The findings herein contradict theoretical expectations and provide new perspectives for understanding the relationship between tenure (in)security and investment decisions.
Supplemental magnolol or honokiol attenuates adverse effects in broilers infected with Salmonella pullorum by modulating mucosal gene expression and the gut microbiota
Authors: Chen Fang , Zhang Hao , Du Encun , Fan Qiwen , Zhao Na , Jin Feng , Zhang Wei , Guo Wanzheng , Huang Shaowen , Wei Jintao
Published: August 2021

Background

Salmonella pullorum is one of the most harmful pathogens to avian species. Magnolol and honokiol, natural compounds extracted from Magnolia officinalis, exerts anti-inflammatory, anti-oxidant and antibacterial activities. This study was conducted to evaluate the effects of dietary supplemental magnolol and honokiol in broilers infected with S. pullorum. A total of 360 one-day-old broilers were selected and randomly divided into four groups with six replicates: the negative control group (CTL), S. pullorum-infected group (SP), and the S. pullorum-infected group supplemented with 300 mg/kg honokiol (SPH) or magnolol (SPM).

Results

The results showed that challenging with S. pullorum impaired growth performance in broilers, as indicated by the observed decreases in body weight (P < 0.05) and average daily gains (P < 0.05), along with increased spleen (P < 0.01) and bursa of Fabricus weights (P < 0.05), serum globulin contents, and the decreased intestine villus height and villus/crypt ratios (P < 0.05). Notably, supplemental magnolol and honokiol attenuated these adverse changes, and the effects of magnolol were better than those of honokiol. Therefore, we performed RNA-Seq in ileum tissues and 16S rRNA gene sequencing of ileum bacteria. Our analysis revealed that magnolol increased the α-diversity (observed species, Chao1, ACE, and PD whole tree) and β-diversity of the ileum bacteria (P < 0.05). In addition, magnolol supplementation increased the abundance of Lactobacillus (P < 0.01) and decreased unidentified Cyanobacteria (P < 0.05) both at d 14 and d 21. Further study confirmed that differentially expressed genes induced by magnolol and honokiol supplementation enriched in cytokine-cytokine receptor interactions, in the intestinal immune network for IgA production, and in the cell adhesion molecule pathways.

Conclusions

Supplemental magnolol and honokiol alleviated S. pullorum-induced impairments in growth performance, and the effect of magnolol was better than that of honokiol, which could be partially due to magnolol’s ability to improve the intestinal microbial and mucosal barrier.
Contemporary Variables that Impact Sleep and Development in Female Adolescent Swimmers and Gymnasts
Authors: Bartholomew Janine , Gilligan Carrie , Spence Ann
Published: August 2021

The effects of sleep on elite athletes in late adolescence and early adulthood have been well documented in a myriad of sports. However, there is underrepresentation of pre-pubertal and young female adolescent athlete research between the ages of 11–17, and specifically female gymnast and swimmers. Neglecting to understand how high energy demand at a young age relates to sleep and restoration may lead to developmental ramifications for this group, as they display physiological dysfunctions like delayed puberty, amenorrhea and are at risk for the female athlete triad or components of the triad. This review aims to summarize the contemporary variables of blue light emitting screens, social media, and caffeine on quality and quantity of sleep in young athletes while identifying gaps in the literature on how these factors impact the target group of young female swimmers and gymnasts.  The implications of this work include sleep hygiene recommendations for increasing duration and quality of sleep, as well as future research with respect to electronic device usage, social media participation, caffeine consumption, and sport engagement in female early adolescent athletes.
Speech-aid prosthesis in velopharyngeal incompetency patient with cleft palate: can speech aids be applicable for adult patient?
Authors: Kang Dong-Cheol , Park Jung-Ho , Seok Hyun , Baek Jin-A , Kim Da-Wa , Ko Seung-O
Published: August 2021

Background

Velopharyngeal incompetence (VPI) therapy for cleft palate (speech therapy alone, speech therapy using speech aids, or combined therapy such as speech therapy using a pharyngeal flap), is more effective in younger patients than in adult patients. Speech therapy is known as very difficult for patients who still have VPI as an adult. Because of the possibility of subsequent speech disorders, the timing of surgery for cleft palate is accelerating. Herein, we present a case of an adult with articulation disorder due to VPI who was treated by speech therapy and a speech-aid prosthesis.

Case presentation

A woman who underwent cleft palate surgery at 8 years of age still had difficulty with articulation due to VPI as a 24-year-old adult because of a lack of continuous speech therapy. We decided to use a speech-aid application using palatal lift, and a reduction program was conducted four times, along with simultaneous speech therapy, over a period of 1 year and 7 months. During the therapy period, she was able to speak normally within a relatively short period of time, and after implementation of the reduction program, the therapy was completed by completely removing the device. Long-term observations have shown normal speech function without recurrence, even after the device was removed.

Conclusion

As seen in this case, speech therapy using speech aids can show a good result for adult patients with cleft palate who missed the usual timing for the treatment of articulation disorders, depending on the situation. Therefore, it is hereby reported as a therapy option worthy of consideration.
FBXW7 gene polymorphism is associated with type 2 diabetes in the Uygur population in Xinjiang, China
Authors: Yan Shi-Qi , Adi Dilare , Liu Cheng , Wang Meng-Meng , Abuzhalihana Jialin , Wu Yun , Fu Zhen-Yan , Yang Yi-Ning , Li Xiao-Mei , Xie Xiang , Liu Fen , Chen Bang-Dang , Ma Yi-Tong
Published: August 2021

Background

FBXW7 gene expression is positively correlated with glycolipid metabolism and is associated with diabetes in animal models. In the current study, we focused on exploring whether genetic variants of the FBXW7 gene were associated with type 2 diabetes (T2DM) and the risk factors for T2DM in Uygur people in Xinjiang, China.

Methods

A total of 2164 Chinese Uygur subjects (673 T2DM patients and 1491 controls) were recruited for our case–control study, and four SNPs (rs10033601, rs2255137, rs2292743 and rs35311955) of the FBXW7 gene were genotyped using the improved multiplex ligation detection reaction (iMLDR) technique.

Results

Our study showed that the genotypes using the overdominant model (GA vs AA + GG) of rs10033601 and using the overdominant model (TA vs TT + AA) of rs2292743 were significantly different between T2DM patients and the controls (P = 0.005 and P = 0.012, respectively). After multivariate adjustments for confounders, the rs10033601 and rs2292743 SNPs were still independent risk factors for T2DM [GA vs AA + GG: odds ratio = 1.35, 95% confidence interval (CI) = 1.12–1.64, P = 0.002; TA vs TT + AA: OR = 1.28, 95% CI = 1.06–1.55, P = 0.011]. Participants within the Chinese Uygur populations and who with the GA genotype of rs10033601 and the TA genotype of rs2292743 were associated with significantly elevated glucose levels.

Conclusions

Our study revealed that both rs10033601 and rs2292743 of the FBXW7 gene were associated with T2DM in the Uygur populations in Xinjiang.
Acceptance and willingness to pay for COVID-19 vaccine among school teachers in Gondar City, Northwest Ethiopia
Authors: Shitu Kegnie , Wolde Maereg , Handebo Simegnew , Kassie Ayenew
Published: August 2021

Background

Vaccination is one of the strategies to prevent the COVID-19 pandemic. COVID-19 vaccine acceptance and cost are important factors affecting its uptake. However, little is known about acceptance and willingness to pay (WTP) for the vaccine.

Objective

This study aimed to assess acceptance and WTP for the COVID-19 vaccine and its associated factors among school teachers.

Methods

An institution-based cross-sectional study was conducted among school teachers. A stratified simple random sampling technique was employed to select the participants. The contingent valuation method was used to assess each respondent’s willingness to pay for the COVID-19 vaccination. A multivariable logistic regression analysis was employed to identify significant factors associated with WTP for the COVID-19 vaccine. A p value of less than 0.05 and a 95% confidence interval were used to declare statistical significance.

Result

A total of 301 school teachers participated with a response rate of 99.6%. The mean age of participants was 39.5 (SD ± 8.7 years). The mean WTP for the COVID-19 vaccine is US$ 4.98 (± US$ 1.6). Less than half (40.8%) of participants were willing to accept the COVID-19 vaccine whereas only 36.9% (95% CI, (31.6%, 42.5%)) of them were WTP for the vaccine. Being male (AOR = 3.23; 95% CI, (1.70, 6.14)), being a private school teacher (AOR = 3.27; 95% CI, (1.76, 6.10)), having high perceived susceptibility to COVID-19 (AOR = 2.69; 95% CI, (1.38, 5.24)), having high perceived seriousness of COVID-19 (AOR = 4.04; 95% CI, (1.80, 9.1)), and having high perceived benefit of COVID-19 vaccine (AOR = 3.0; 95% CI, (1.41, 6.34) were significant factors associated with WTP for COVID-19 vaccination.

Conclusion and recommendation

The magnitude of acceptance and WTP for the COVID-19 vaccine was low among school teachers. Male sex, private school affiliation, high perceived risk of COVID-19, and benefit of COVID-19 vaccine were the most important factors affecting school teacher’s WTP for COVID-19 vaccine. Thus, health communication programs should give due emphasis to raising teacher’s perceived risk of COVID-19 and the benefit of the COVID-19 vaccine to improve their acceptance and WTP for the vaccine. Furthermore, subsidizing the cost of the vaccine may improve their WTP.
Adrenal Corticosteroid Perturbation by the Endocrine Disruptor BDE-47 in a Human Adrenocortical Cell Line and Male Rats
Authors: Dungar Benjamin M , Schupbach Chad D , Jacobson Jessie R , Kopf Phillip G
Published: August 2021

Polybrominated diphenyl ethers (PBDEs) have been previously shown to alter various endocrine biosynthetic pathways. Growing epidemiological evidence suggests that PBDEs alter cardiovascular function. The goal of this study was to examine the effects of BDE-47 on adrenal corticosteroid pathways that play vital roles in cardiovascular homeostasis and pathophysiology. The effect of BDE-47 on aldosterone and cortisol secretion was characterized in a human adrenocortical cell line. HAC15 cells were exposed to various concentrations of BDE-47 (1 nM to 100 μM). Cell viability, corticosteroid secretion, gene expression of enzymes involved in corticosteroid synthesis, and metabolic activity was examined. Additionally, Sprague Dawley male rats were orally exposed to BDE-47 (10 or 100 µg/kg), 5 days per week for 16 weeks. Organ weights and plasma corticosteroid levels were measured. In HAC15 cells, basal and stimulated aldosterone and cortisol secretion was significantly increased by BDE-47. Gene expression of several enzymes involved in corticosteroid synthesis and mitochondrial metabolism also increased. In Sprague Dawley rats, adrenal but not heart, kidney, or liver weights, were significantly increased in BDE-47 treatment groups. Plasma corticosterone levels were significantly increased in the 100 µg BDE-47/kg treatment group. No change in plasma aldosterone levels were observed with BDE-47 exposure. These data indicate that BDE-47 disrupts the regulation of corticosteroid secretion and provides further evidence that PBDEs are potential endocrine disruptors. Future studies will determine the underlying molecular mechanism of altered corticosteroid production and examine whether these alterations result in underlying cardiovascular disease in our rodent model of 16-week BDE-47 exposure.
Arf GTPase-activating proteins SMAP1 and AGFG2 regulate the size of Weibel-Palade bodies and exocytosis of von Willebrand factor
Authors: Watanabe Asano , Hataida Hikari , Inoue Naoya , Kamon Kosuke , Baba Keigo , Sasaki Kuniaki , Kimura Rika , Sasaki Honoka , Eura Yuka , Ni Wei-Fen , Shibasaki Yuji , Waguri Satoshi , Kokame Koichi , Shiba Yoko
Published: August 2021

Arf GTPase-Activating proteins (ArfGAPs) mediate the hydrolysis of GTP bound to ADP-ribosylation factors (Arfs), which are critical to form transport intermediates. ArfGAPs have been thought to be negative regulators of Arfs; however, accumulating evidence indicates that ArfGAPs are important for cargo sorting and promote membrane traffic. Weibel-Palade bodies (WPBs) are cigar-shaped secretory granules in endothelial cells that contain von Willebrand factor (vWF) as their main cargo. WPB biogenesis at the Golgi was reported to be regulated by Arf and their regulators, but the role of ArfGAPs has been unknown. In this study, we performed siRNA screening of ArfGAPs to investigate the role of ArfGAPs in the biogenesis of WPBs. We found two ArfGAPs, SMAP1 and AGFG2, to be involved in WPB size and vWF exocytosis, respectively. SMAP1 depletion resulted in small-sized WPBs, and the lysosomal inhibitor leupeptin recovered the size of WPBs. The results indicate that SMAP1 functions in preventing the degradation of cigar-shaped WPBs. On the other hand, AGFG2 downregulation resulted in the inhibition of vWF secretion upon Phorbol 12-myristate 13-acetate (PMA) or histamine stimulation, suggesting that AGFG2 plays a role in vWF exocytosis. Our study revealed unexpected roles of ArfGAPs in vWF transport.
Enhanced RhoA signalling stabilizes E-cadherin in migrating epithelial monolayers
Authors: Gupta Shafali , Duszyc Kinga , Verma Suzie , Budnar Srikanth , Liang Xuan , Gomez Guillermo A. , Marcq Philippe , Noordstra Ivar , Yap Alpha S.
Published: August 2021

Epithelia migrate as physically coherent populations of cells. Previous studies have revealed that mechanical stress accumulates in these cellular layers as they move. These stresses are characteristically tensile in nature and have often been inferred to arise when moving cells pull upon the cell–cell adhesions that hold them together. We now report that epithelial tension at adherens junctions between migrating cells also increases due to an increase in RhoA-mediated junctional contractility. We found that active RhoA levels were stimulated by p114 RhoGEF (also known as ARHGEF18) at the junctions between migrating MCF-7 monolayers, and this was accompanied by increased levels of actomyosin and mechanical tension. Applying a strategy to restore active RhoA specifically at adherens junctions by manipulating its scaffold, anillin, we found that this junctional RhoA signal was necessary to stabilize junctional E-cadherin (CDH1) during epithelial migration and promoted orderly collective movement. We suggest that stabilization of E-cadherin by RhoA serves to increase cell–cell adhesion to protect against the mechanical stresses of migration.

This article has an associated First Person interview with the first author of the paper.
Large-Scale Fandom-based Gamification Intervention to Increase Physical Activity: A Quasi-experimental Study
Authors: KAMADA MASAMITSU , HAYASHI HANA , SHIBA KOICHIRO , TAGURI MASATAKA , KONDO NAOKI , LEE I-MIN , KAWACHI ICHIRO
Published: August 2021

Purpose

Gamification, the use of game design elements in nongame contexts, in combination with insights from behavioral economics, has been applied increasingly to behavior change interventions. However, little is known about the effectiveness or scalability of this approach, especially in the long term. We tested a large-scale smartphone-based intervention to encourage physical activity among Japanese baseball fans using gamification techniques that leveraged fandom and interteam competition inherent in sports.

Methods

A quasi-experimental study was conducted among fans of the Japanese Pacific League. The app, Pa-League Walk, included gamification elements, such as competition between opposing teams’ fans based on total daily step counts on game days (>60,000 free downloads since March 2016). We analyzed daily steps of 20,052 app users, supplemented by online survey data of 274 users and 613 matched controls. Difference-in-differences estimators evaluated change in daily steps before and after app installation in users versus matched controls.

Results

Users’ daily steps increased by 574 (95% confidence interval, 83–1064) steps 3 months after installation, compared with controls. The increase was maintained for up to 9 months (559 (99–1018) more steps per day vs baseline), attenuating over a longer follow-up. Positive effect modification was found by high-frequency of the app use (P < 0.001) but not by other covariables (P ≥ 0.14) such as education or income. Days with 10,000-step achievement increased from 24.4% to 27.5% after the additional introduction of incentives (digital player photographs; P < 0.001).

Conclusions

Using existing fandom and solidarity, the gamification app increased physical activity at scale among baseball fans, including people with lower socioeconomic status underrepresented in traditional health programs.