bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimal timing of primaquine to reduce Plasmodium falciparum gametocyte carriage when co-administered with artemether–lumefantrine
Authors: Shekalaghe Seif , Mosha Dominic , Hamad Ali , Mbaga Thabit A. , Mihayo Michael , Bousema Teun , Drakeley Chris , Abdulla Salim
Published: January 2020

Background

Primaquine is an important gametocytocidal drug that is combined with conventional malaria treatment for prevention of Plasmodium falciparum malaria transmission. Primaquine has been administered together on the first or the last day of conventional treatment but the impact of primaquine timing has never been examined. This study aimed to assess safety, efficacy and optimal timing of single full-dose (0.75 mg/kg) primaquine when added to a standard 6-dose regimen of artemether–lumefantrine (AL).

Methods

In an individual-level randomized controlled trial, enrolled participants who were G6PD normal and had uncomplicated P. falciparum malaria were randomly assigned to receive: AL only; AL and a single 0.75 mg/kg primaquine dose on the first day of AL (day 1); or AL and single 0.75 mg//kg primaquine on the last day of AL (day 3). On days 2, 3, 4, 8, 11 and 15, gametocytes were assessed and quantified by microscope and quantitative nuclear acid sequence based quantification (QT-NASBA).

Results

Overall, 111 participants aged between 3 and 17 years were randomly allocated to receive AL only (36) or combined with primaquine on day 1 (38), or primaquine on day 3 (37). Day 4 gametocyte prevalence in AL + day 1 primaquine was half the level seen in either AL + day 3 primaquine or AL only arm (11% [4/35] vs 26% [8/31] and 27% [8/30], respectively) albeit not statistically significant. A similar trend of lower gametocyte in the AL + day 1 primaquine verses AL + day 3 primaquine or AL only arm was observed in mean gametocyte density. Mean (sd) haemoglobin level in AL + day 3 primaquine arm recovered from -0.42(1.2) g/dl on day 2 to 0.35 (1.5) g/dl on day 15 of follow up. This was not the case in AL only and AL + day 1 primaquine arms during the same follow-up period, although the difference was not statistically significant (p = 318). No serious adverse events reported in the study. Across arms, 23% (26/111) of participants reported a total of 31 mild adverse events and the difference was not statistically significant (p = 0.477).

Conclusion

Primaquine administration on the first day of AL is well tolerated and as safe as later administration. Whilst the World Health Organization currently recommends a lower dose of primaquine (0.25 mg/kg), the findings are supportive of early primaquine administration when combined with artemisinin-combination therapy.

ClinicalTrials.gov Registration NCT01906788
Elaboration of a multimodal MRI-based radiomics signature for the preoperative prediction of the histological subtype in patients with non-small-cell lung cancer
Authors: Tang Xing , Xu Xiaopan , Han Zhiping , Bai Guoyan , Wang Hong , Liu Yang , Du Peng , Liang Zhengrong , Zhang Jian , Lu Hongbing , Yin Hong
Published: January 2020

Background

Non-invasive discrimination between lung squamous cell carcinoma (LUSC) and lung adenocarcinoma (LUAD) subtypes of non-small-cell lung cancer (NSCLC) could be very beneficial to the patients unfit for the invasive diagnostic procedures. The aim of this study was to investigate the feasibility of utilizing the multimodal magnetic resonance imaging (MRI) radiomics and clinical features in classifying NSCLC. This retrospective study involved 148 eligible patients with postoperative pathologically confirmed NSCLC. The study was conducted in three steps: (1) feature extraction was performed using the online freely available package with the multimodal MRI data; (2) feature selection was performed using the Student’s t test and support vector machine (SVM)-based recursive feature elimination method with the training cohort (n = 100), and the performance of these selected features was evaluated using both the training and the validation cohorts (n = 48) with a non-linear SVM classifier; (3) a Radscore model was then generated using logistic regression algorithm; (4) Integrating the Radscore with the semantic clinical features, a radiomics–clinical nomogram was developed, and its overall performance was evaluated with both cohorts.

Results

Thirteen optimal features achieved favorable discrimination performance with both cohorts, with area under the curve (AUC) of 0.819 and 0.824, respectively. The radiomics–clinical nomogram integrating the Radscore with the independent clinical predictors exhibited more favorable discriminative power, with AUC improved to 0.901 and 0.872 in both cohorts, respectively. The Hosmer–Lemeshow test and decision curve analysis results furtherly showed good predictive precision and clinical usefulness of the nomogram.

Conclusion

Non-invasive histological subtype stratification of NSCLC can be done favorably using multimodal MRI radiomics features. Integrating the radiomics features with the clinical features could further improve the performance of the histological subtype stratification in patients with NSCLC.
Complete resolution of urinary incontinence with treatment improved the health-related quality of life of children with functional daytime urinary incontinence: a prospective study
Authors: Ikeda Hirokazu , Oyake Chisato , Oonuki Yuta , Fuyama Masaki , Watanabe Tsuneki , Kyoda Takashi , Tamura Setuko
Published: January 2020

Background

To assess the health-related quality of life (HRQOL) of children with daytime urinary incontinence (DUI) based on pre- and post-treatment self-reports and parent proxy-reports.

Methods

The study population comprised 117 children with at least one episode of DUI per week and their caregivers as well as 999 healthy children (control group). The Pediatric Quality of Life Inventory 4.0 (PedsQL) questionnaire was administered to assess the HRQOL of children. To assess the degree of improvement in HRQOL, we categorized children into two groups: group A achieved complete response (CR) to treatment within 12 months and group B did not achieve CR within 12 months. CR was defined as the complete resolution of symptoms or alleviation of symptoms to < 1 DUI episode/month.

Results

Valid responses were collected from 84 children [53 boys and 31 girls; mean age: 7.9 ± 1.5 years (range, 6–12)]. Sixty-two patients (73.8%) were classified into group A and 22 (26.1%) into group B. Based on self-reports, significant post-treatment improvement was observed in the scores of all PedsQL items (mean total score: 82.2 ± 11.3 vs. 87.2 ± 9.8; P = 0.003). Group A showed significant improvement in the scores of all PedsQL items after achievement of CR based on child self-reports; however, this was improvement not observed in group B.

Conclusions

To the best of our knowledge, this is the first study to demonstrate the complete resolution of DUI with treatment for improving the HRQOL of these children.
Correction to: Demographic, clinical, and treatment characteristics of the juvenile primary fibromyalgia syndrome cohort enrolled in the Childhood Arthritis and Rheumatology Research Alliance Legacy Registry
Authors:   and For the CARRA Registry Investigators, Weiss Jennifer E. , Schikler Kenneth N. , Boneparth Alexis D. , Connelly Mark
Published: January 2020

Following publication of the original article [1], we have been notified that the corresponding author’s given name is spelled incorrectly.
Designing clinical practice feedback reports: three steps illustrated in Veterans Health Affairs long-term care facilities and programs
Authors: Landis-Lewis Zach , Kononowech Jennifer , Scott Winifred J. , Hogikyan Robert V. , Carpenter Joan G. , Periyakoil V. S. , Miller Susan C. , Levy Cari , Ersek Mary , Sales Anne
Published: January 2020

Background

User-centered design (UCD) methods are well-established techniques for creating useful artifacts, but few studies illustrate their application to clinical feedback reports. When used as an implementation strategy, the content of feedback reports depends on a foundational audit process involving performance measures and data, but these important relationships have not been adequately described. Better guidance on UCD methods for designing feedback reports is needed. Our objective is to describe the feedback report design method for refining the content of prototype reports.

Methods

We propose a three-step feedback report design method (refinement of measures, data, and display). The three steps follow dependencies such that refinement of measures can require changes to data, which in turn may require changes to the display. We believe this method can be used effectively with a broad range of UCD techniques.

Results

We illustrate the three-step method as used in implementation of goals of care conversations in long-term care settings in the U.S. Veterans Health Administration. Using iterative usability testing, feedback report content evolved over cycles of the three steps. Following the steps in the proposed method through 12 iterations with 13 participants, we improved the usability of the feedback reports.

Conclusions

UCD methods can improve feedback report content through an iterative process. When designing feedback reports, refining measures, data, and display may enable report designers to improve the user centeredness of feedback reports.
The scale affects our view on the identification and distribution of microbial communities in ticks
Authors: Pollet Thomas , Sprong Hein , Lejal Emilie , Krawczyk Aleksandra I. , Moutailler Sara , Cosson Jean-Francois , Vayssier-Taussat Muriel , Estrada-Peña Agustín
Published: January 2020

Ticks transmit the highest variety of pathogens impacting human and animal health worldwide. It is now well established that ticks also harbour a microbial complex of coexisting symbionts, commensals and pathogens. With the development of high throughput sequencing technologies, studies dealing with such diverse bacterial composition in tick considerably increased in the past years and revealed an unexpected microbial diversity. These data on diversity and composition of the tick microbes are increasingly available, giving crucial details on microbial communities in ticks and improving our knowledge on the tick microbial community. However, consensus is currently lacking as to which scales (tick organs, individual specimens or species, communities of ticks, populations adapted to particular environmental conditions, spatial and temporal scales) best facilitate characterizing microbial community composition of ticks and understanding the diverse relationships among tick-borne bacteria. Temporal or spatial scales have a clear influence on how we conduct ecological studies, interpret results, and understand interactions between organisms that build the microbiome. We consider that patterns apparent at one scale can collapse into noise when viewed from other scales, indicating that processes shaping tick microbiome have a continuum of variability that has not yet been captured. Based on available reports, this review demonstrates how much the concept of scale is crucial to be considered in tick microbial community studies to improve our knowledge on tick microbe ecology and pathogen/microbiota interactions.
MicroRNA-126 protects against vascular injury by promoting homing and maintaining stemness of late outgrowth endothelial progenitor cells
Authors: Pei Chong Zhe , Liu Bo , Li Ye Ting , Fang Lu , Zhang Yi , Li Yi Gang , Meng Shu
Published: January 2020

Background

Endothelial progenitor cells (EPCs) contribute to reendothelialization and neovascularization and protect against vascular injury and ischemia of various organs. We have previously shown downregulation of microRNA (miR)-126 in EPCs from diabetic patients, which contributes to dysfunction of EPCs including impaired migratory ability. The aims of the present study were to examine (1) in vitro the effects of miR-126 on the homing and stemness of late outgrowth EPCs (LOCs), along with relevant signaling pathways, and (2) in vivo the effects of modulating LOCs by manipulating miR-126 expression on LOC homing and reendothelialization of injured arteries in GK rats (a non-obese diabetes model).

Methods

Rat bone marrow-derived LOCs were transfected with miR-126 inhibitor or lentiviral vectors expressing miR-126. LOC migration was determined by transwell migration assay. CXCR4 expression was measured by real-time PCR, Western blotting, and confocal microscopy while related signaling pathway proteins were measured by Western Blotting. Stemness gene expression, and gene and protein expression and promoter activity of KLF-8 were also measured. LOCs transfected with lenti-miR-126 or miR-126 inhibitor were injected into GK rats with carotid artery injury, and then vascular reendothelialization and the extent of intimal hyperplasia were examined.

Results

Lenti-miR-126 increased while miR-126 inhibitor decreased LOC migration and CXCR4 expression on LOCs. miR-126 positively regulated p-ERK, VEGF, p-Akt, and eNOS protein expression, and inhibitors of these proteins blocked miR-126-induced CXCR4 expression and also reduced LOC migration. Overexpression of miR-126 promoted while inhibition of miR-126 suppressed stemness gene expression in LOCs. miR-126 also inhibited gene and protein expression and promoter activity of KLF-8 while shRNA-mediated knockdown of KLF-8 increased stemness gene expression. Upregulation of stemness gene expression by miR-126 overexpression was completely abrogated by co-transfection of lenti-KLF-8 and lenti-miR-126 into LOCs. In GK rats, transplantation of LOCs overexpressing miR-126 enhanced LOC homing and reendothelialization and decreased intimal hyperplasia of injured arteries.

Conclusion

Our results indicate that miR-126 protects against vascular injury by promoting CXCR4 expression and LOC homing via ERK/VEGF and Akt/eNOS signaling pathways and maintaining stemness via targeting KLF-8.
LncRNA-NEAT1 from the competing endogenous RNA network promotes cardioprotective efficacy of mesenchymal stem cell-derived exosomes induced by macrophage migration inhibitory factor via the miR-142-3p/FOXO1 signaling pathway
Authors: Chen Hanbin , Xia Wenzheng , Hou Meng
Published: January 2020

Aims

Extracellular vesicles, especially exosomes, have emerged as key mediators of intercellular communication with the potential to improve cardiac function as part of cell-based therapies. We previously demonstrated that the cardioprotective factor, macrophage migration inhibitory factor (MIF), had an optimizing effect on mesenchymal stem cells (MSCs). The aim of this study was to determine the protective function of exosomes derived from MIF-pretreated MSCs in cardiomyocytes and to explore the underlying mechanisms.

Methods and results

Exosomes were isolated from control MSCs (exosome) and MIF-pretreated MSCs (exosomeMIF), and delivered to cardiomyocytes subjected to H2O2in vitro. Regulatory long non-coding RNAs (lncRNAs) activated by MIF pretreatment were explored using genomics approaches. ExosomeMIFprotected cardiomyocytes from H2O2-induced apoptosis. Mechanistically, we identified lncRNA-NEAT1 as a mediator of exosomeMIFby regulating the expression of miR-142-3p and activating Forkhead class O1 (FOXO1). The cardioprotective effects of exosomeMIFwere consistently abrogated by depletion of lncRNA-NEAT1, by overexpression of miR-142-3p, or by FOXO1 silencing. Furthermore, exosomeMIFinhibited H2O2-induced apoptosis through modulating oxidative stress.

Conclusions

Exosomes obtained from MIF-pretreated MSCs have a protective effect on cardiomyocytes. The lncRNA-NEAT1 functions as an anti-apoptotic molecule via competitive endogenous RNA activity towards miR-142-3p. LncRNA-NEAT1/miR-142-3p/FOXO1 at least partially mediates the cardioprotective roles of exosomeMIFin protecting cardiomyocytes from apoptosis.
The emerging specialty of perioperative medicine: a UK survey of the attitudes and behaviours of anaesthetists
Authors: Partridge J. S. L. , Rogerson A. , Joughin A. L. , Walker D. , Simon J. , Swart M. , Dhesi J. K.
Published: January 2020

Background

In 2014, the Royal College of Anaesthetists (RCoA) launched the Perioperative Medicine Programme to facilitate the delivery of best preoperative, intraoperative and postoperative care through implementation of evidence-based medicine to reduce variation and improve postoperative outcomes. However, variation exists in the establishment of perioperative medicine services in the UK. This survey explored attitudes and behaviours of anaesthetists towards perioperative medicine, described current anaesthetic-led perioperative medicine services across the UK and explored barriers to anaesthetic involvement in perioperative medicine.

Methods

Survey content based on the RCoA vision document was refined and validated using an expert panel. An anonymous electronic survey was then sent by email to the members of the RCoA.

Results

Seven hundred fifty-eight UK anaesthetists (4.5% of the RCoA mailing list) responded to the survey. Of these, 64% considered themselves a perioperative doctor, with 65% having changed local services in response to the RCoA vision. Barriers to developing perioperative medicine included insufficient time (75%) and inadequate training (51%). Three quarters of respondents advocate anaesthetists leading the development of perioperative medicine.

Conclusions

Despite evidence of emerging services, this survey describes barriers to ongoing development of perioperative medicine. Facilitators may include increased clinical exposure, targeted education and training and collaborative working with other specialties.
Experimental Investigation on Flexural Behavior of Reinforced Ultra High Performance Concrete Low-Profile T-Beams
Authors: Qiu Minghong , Shao Xudong , Wille Kay , Yan Banfu , Wu Jiajia
Published: January 2020

The flexural behavioral properties of ultra high performance concrete (UHPC) low-profile T-beams reinforced with a combination of steel fibers and steel reinforcing bars were investigated in this paper. Five large scale T-beams were tested and analyzed regarding their deflection, ductility, strain, curvature, load capacity and crack development. The experimental variables include the reinforcement ratio, the slenderness (length to diameter ratio) of the fiber reinforcements, and the fiber type. The experiments showed that all specimens exhibit flexural failure with the yielding of steel bars and excessive expansion of flexural crack, and the compression zone in the reinforced UHPC low-profile T-beam is not crushed because of the ultra high compressive strength and area of UHPC. In addition, it was concluded that using hooked-end fibers can effectively increase the specimen’s durability-based cracking load in comparison to straight fibers of same slenderness, whereas the reinforcement ratio and the slenderness of the fibers have little influence on this. Increasing the reinforcement ratio and using hooked-end instead of straight fibers increase the load capacity and bending stiffness of the specimen, as well as reduces the crack width at comparable applied load. A model was established to compute the ultimate capacity of UHPC low-profile T-beams and the prediction agrees well with the experimental results in the present and published investigations.
Energetic performance index improvement after Glenn and Damus-Kaye-Stansel procedure using vector flow mapping analysis: a case report
Authors: Kainuma Atsushi , Akiyama Koichi , Naito Yoshifumi , Hayase Kazuma , Hongu Hisayuki , Itatani Keiichi , Yamagishi Masaaki , Sawa Teiji
Published: January 2020

Background

Echocardiography vector flow mapping can assess dynamic flow to treat congenital heart diseases. We evaluated intracardiac flow, energy loss, left ventricular output kinetic energy, and energetic performance index using vector flow mapping during Glenn and Damus-Kaye-Stansel procedures in order to assess the efficacy of the surgery.

Case presentation

A 9-month-old boy underwent Glenn and Damus-Kaye-Stansel procedures. The energy loss depends on the left ventricular preload; therefore, energy loss decreased after the Glenn procedure. After the Damus-Kaye-Stansel procedure, the kinetic energy would increase owing to the integrated systemic outflow; however, in our case, kinetic energy decreased, which was potentially explained by the fact that kinetic energy also depends on the left ventricular preload. After the Glenn and Damus-Kaye-Stansel procedures, we detected an improvement in energetic performance index, indicating that the cardiac workload improved as well.

Conclusion

We revealed the efficiency of the Glenn and Damus-Kaye-Stansel procedures using vector flow mapping.
Fluid-solid coupled full-waveform inversion in the curvilinear coordinates for ocean-bottom cable data
Authors: Qu Yingming , Guan Zhe , Li Jinli , Li Zhenchun
Published: January 2020

Marine seismic exploration with ocean-bottom cable technology is able to record P- and S-wave information simultaneously. Elastic full-waveform inversion (EFWI) uses P- and S-waves to invert multiple parameters with adequate amplitude information and complete illumination of the subsurface. To calculate the wavefield within EFWI, we use different formats of wave equations in fluid and solid mediums and an appropriate boundary condition to convert waves on the interface. This partitioned simulation scheme is more stable and efficient than the traditional integrated simulation scheme. However, if the fluid-solid coupled medium has an extremely irregular interface, the conventional finite-difference method with rectangular grids cannot obtain accurate source and receiver wavefields. We use the curvilinear coordinates to overcome this limitation. In the curvilinear coordinates, the irregular interface can be transformed into a horizontal interface. To reduce the crosstalk of inverted P- ([Formula: see text]) and S-velocities ([Formula: see text]), we derive the gradient formulas of [Formula: see text] and [Formula: see text] based on P- and S-wave mode separation in the curvilinear coordinates, and, finally, we develop a 2D curvilinear-grid-based fluid-solid separated-wavefield EFWI (CFS-SEFWI) method. Numerical examples that include an anomaly model and a modified Marmousi II model demonstrate that CFS-SEFWI overcomes the influence of the irregular fluid-solid interface and efficiently reduces crosstalk effects between [Formula: see text] and [Formula: see text]. Our results also demonstrate that this method is less sensitive to noise compared to the conventional CFS FWI method without separating wave modes.
Phase IB/II Trial of Lenvatinib Plus Pembrolizumab in Patients With Advanced Renal Cell Carcinoma, Endometrial Cancer, and Other Selected Advanced Solid Tumors
Authors: Taylor Matthew H. , Lee Chung-Han , Makker Vicky , Rasco Drew , Dutcus Corina E. , Wu Jane , Stepan Daniel E. , Shumaker Robert C. , Motzer Robert J.
Published: January 2020

PURPOSE

Modulation of vascular endothelial growth factor–mediated immune suppression via angiogenesis inhibition may augment the activity of immune checkpoint inhibitors. We report results from the dose-finding and initial phase II expansion of a phase Ib/II study of lenvatinib plus pembrolizumab in patients with selected advanced solid tumors.

METHODS

Eligible patients had metastatic renal cell carcinoma (RCC), endometrial cancer, squamous cell carcinoma of the head and neck (SCCHN), melanoma, non–small-cell lung cancer (NSCLC), or urothelial cancer. The primary objective of phase Ib was to determine the maximum tolerated dose (MTD) for lenvatinib plus pembrolizumab (200 mg intravenously every 3 weeks). In the preplanned phase II cohort expansion, the primary objective was objective response rate at week 24 (ORRweek 24) at the recommended phase II dose.

RESULTS

Overall, 137 patients were enrolled during phase Ib (n = 13) and the initial phase II expansion (n = 124). Two dose-limiting toxicities (DLTs; grade 3 arthralgia and grade 3 fatigue) were reported in the initial dose level (lenvatinib 24 mg/d plus pembrolizumab). No DLTs were observed in the subsequent dose–de-escalation cohort, establishing the MTD and recommended phase II dose at lenvatinib 20 mg/d plus pembrolizumab. ORRweek24 was as follows: RCC, 63% (19/30; 95% CI, 43.9% to 80.1%); endometrial cancer, 52% (12/23; 95% CI, 30.6% to 73.2%); melanoma, 48% (10/21; 95% CI, 25.7% to 70.2%); SCCHN, 36% (8/22; 95% CI, 17.2% to 59.3%); NSCLC, 33% (7/21; 95% CI, 14.6% to 57.0%); and urothelial cancer 25% (5/20; 95% CI, 8.7% to 49.1%). The most common treatment-related adverse events were fatigue (58%), diarrhea (52%), hypertension (47%), and hypothyroidism (42%).

CONCLUSION

Lenvatinib plus pembrolizumab demonstrated a manageable safety profile and promising antitumor activity in patients with selected solid tumor types.
A Dual Reporter EndoC-βH1 Human β-Cell Line for Efficient Quantification of Calcium Flux and Insulin Secretion
Authors: Cardenas-Diaz Fabian L , Leavens Karla F , Kishore Siddharth , Osorio-Quintero Catherine , Chen Yi-Ju , Stanger Ben Z , Wang Pei , French Deborah , Gadue Paul
Published: January 2020

Human in vitro model systems of diabetes are critical to both study disease pathophysiology and offer a platform for drug testing. We have generated a set of tools in the human β-cell line EndoC-βH1 that allows the efficient and inexpensive characterization of β-cell physiology and phenotypes driven by disruption of candidate genes. First, we generated a dual reporter line that expresses a preproinsulin–luciferase fusion protein along with GCaMP6s. This reporter line allows the quantification of insulin secretion by measuring luciferase activity and calcium flux, a critical signaling step required for insulin secretion, via fluorescence microscopy. Using these tools, we demonstrate that the generation of the reporter human β-cell line was highly efficient and validated that luciferase activity could accurately reflect insulin secretion. Second, we used a lentiviral vector carrying the CRISPR-Cas9 system to generate candidate gene disruptions in the reporter line. We also show that we can achieve gene disruption in ~90% of cells using a CRISPR–Cas9 lentiviral system. As a proof of principle, we disrupt the β-cell master regulator, PDX1, and show that mutant EndoC-βH1 cells display impaired calcium responses and fail to secrete insulin when stimulated with high glucose. Furthermore, we show that PDX1 mutant EndoC-βH1 cells exhibit decreased expression of the β-cell-specific genes MAFA and NKX6.1 and increased GCG expression. The system presented here provides a platform to quickly and easily test β-cell functionality in wildtype and cells lacking a gene of interest.
Non-canonical regulation of homologous recombination DNA repair by the USP9X deubiquitylase
Authors: O'Dea Rachel , Santocanale Corrado
Published: January 2020

In order to prevent the deleterious effects of genotoxic agents, cells have developed complex surveillance mechanisms and DNA repair pathways that allow them to maintain genome integrity. The ubiquitin-specific protease 9X (USP9X) contributes to genome stability during DNA replication and chromosome segregation. Depletion of USP9X leads to DNA double-strand breaks, some of which are triggered by replication fork collapse. Here, we identify USP9X as a novel regulator of homologous recombination (HR) DNA repair in human cells. By performing cellular HR reporter, irradiation-induced focus formation and colony formation assays, we show that USP9X is required for efficient HR. Mechanistically, we show USP9X is important to sustain the expression levels of key HR factors, namely BRCA1 and RAD51 through a non-canonical regulation of their mRNA abundance. Intriguingly, we find that the contribution of USP9X to BRCA1 and RAD51 expression is independent of its known catalytic activity. Thus, this work identifies USP9X as a regulator of HR, demonstrates a novel mechanism by which USP9X can regulate protein levels, and provides insights in to the regulation of BRCA1 and RAD51 mRNA.

This article has an associated First Person interview with the first author of the paper.
The gap junction protein connexin 43 controls multiple aspects of cranial neural crest cell development
Authors: Jourdeuil Karyn , Taneyhill Lisa A.
Published: January 2020

Gap junctions are intercellular channels between cells that facilitate cell–cell communication. Connexin 43 (Cx43; also known as GJA1), the predominant gap junction protein in vertebrates, is expressed in premigratory cranial neural crest cells and is maintained throughout the neural crest cell epithelial-to-mesenchymal transition (EMT), but its function in these cells is unknown. To this end, we used a combination of in vivo and ex vivo experiments to assess gap junction formation, and Cx43 function, in chick cranial neural crest cells. Our results demonstrate that gap junctions exist between premigratory and migratory cranial neural crest cells and depend on Cx43 for their function. In the embryo, Cx43 knockdown just prior to EMT delays the emergence of Cx43-depleted neural crest cells from the neural tube, but these cells eventually successfully emigrate and join the migratory stream. This delay can be rescued by introduction of full-length Cx43 into Cx43-depleted cells. Furthermore, Cx43 depletion reduces the size of the premigratory neural crest cell domain through an early effect on neural crest cell specification. Collectively, these data identify new roles for Cx43 in chick cranial neural crest cell development.
DLC1 is a direct target of activated YAP/TAZ that drives collective migration and sprouting angiogenesis
Authors: van der Stoel Miesje , Schimmel Lilian , Nawaz Kalim , van Stalborch Anne-Marieke , de Haan Annett , Klaus-Bergmann Alexandra , Valent Erik T. , Koenis Duco S. , van Nieuw Amerongen Geerten P. , de Vries Carlie J. , de Waard Vivian , Gloerich Martijn , van Buul Jaap D. , Huveneers Stephan
Published: January 2020

Endothelial YAP/TAZ (YAP is also known as YAP1, and TAZ as WWTR1) signaling is crucial for sprouting angiogenesis and vascular homeostasis. However, the underlying molecular mechanisms that explain how YAP/TAZ control the vasculature remain unclear. This study reveals that the focal adhesion protein deleted-in-liver-cancer 1 (DLC1) is a direct transcriptional target of the activated YAP/TAZ–TEAD complex. We find that substrate stiffening and VEGF stimuli promote expression of DLC1 in endothelial cells. In turn, DLC1 expression levels are YAP and TAZ dependent, and constitutive activation of YAP is sufficient to drive DLC1 expression. DLC1 is needed to limit F-actin fiber formation, integrin-based focal adhesion lifetime and integrin-mediated traction forces. Depletion of endothelial DLC1 strongly perturbs cell polarization in directed collective migration and inhibits the formation of angiogenic sprouts. Importantly, ectopic expression of DLC1 is sufficient to restore migration and angiogenic sprouting in YAP-depleted cells. Together, these findings point towards a crucial and prominent role for DLC1 in YAP/TAZ-driven endothelial adhesion remodeling and collective migration during angiogenesis.

This article has an associated First Person interview with the first author of the paper.
Contemporary stroke care delivered in a specialised stroke bay, a neurology ward and a medical ward in Greece
Authors: Theofanidis Dimitrios , Fountouki Antigoni , Savopoulos Christos , Hatzitolios Apostolos , Fitsioris Xenofon , Rudolf Jiobst
Published: January 2020

Introduction: Currently in Greece there are only two ‘stroke bays’ (SBs) and their efficacy compared to standard ward treatment has not been evaluated.Aim: To provide baseline data on stroke case-mix and compare death rates in two Greek hospitals (A+B, i.e. with & without a SB).Material and Methods: One hundrend sixty four acute stroke patients from two hospitals were consecutively selected in order to explore approaches to stroke care in three different types of care delivery settings. A descriptive design with group comparisons was employedand data gathering included patient case mix, age and length of stay (LOS), neurological status and death rates. Kaplan-Meier curves we reused for survival analysis and independent samples t test and Z test for group comparisons at p<0.05.Results: Of the 164 patients, 88 (44.4%, female) werein hospital A and 76 (42.1%, female) in hospital B. The mean age was 65.7 and 69.1 years respectively, LOS was 7.6 (3-18) and 7.3 days (1-26), while death rate was 6.8% and 10.5% respectively.Discussion: Survival analysis for the two hospitalsshowed that there are no statistically significant differences regarding death and survival rates between the two hospitals, even when one had a SB. Yet, the SB shows a statistically significant reduction in overall LOS.Conclusions: In societies where stroke units are not yet established, a SB might be an economical way of making use of (limited) available resources and raising nursing and medical standards of care by motivating the full potential of staff involved.
ITANGIBLE REWARDS: FROM THEROTICAL MEHODS TO PRACTICE IN THE MODERN CONDITIONS IN ORGANIZATIONS
Authors: Ivanova I.
Published: January 2020

This article presents modern aspects of intangible motivation and labor stimulation in modern academic highlights and real practice in organizations. The extra attention is paid to the detecting of intangible benefits in organizations, which depends on the influence of external and internal factors that determine the need to use an individual approach when developing motivational programs. Due to implementing informational and communicational technologies in the process of digitalization, the need for social and psychological support of personnel is actualized, which, according to the author, should be taken into account in the system of motivation and stimulation of labor. Organizational and economic changes, generational shifts, change of values, the impact of globalization, scientific and technological progress, changing needs, and the active spread of electronic resources affect the relations between personnel and labor, increasing the importance of studying the motives of their labor behavior in new circumstances. The author considers motivation and stimulation as a direction in working with people, mainly focuses on finding reserves associated with optimal spending and obtaining effective results, which can ensure the use of intangible benefits. Furthermore, based on the existing theory and practice of motivation and stimulation, the author suggests integrating it into the modern environment, pointing to the need to preserve the already accumulated experience and increase it in the conditions of digitalization.
Psychological Culture of a Manager As a Psychological Phenomenon of Professional Competence
Authors: Koneva D.
Published: January 2020

The phenomenon of professional competence has become fi rmly established in the practice of human resource management of modern organizations. It plays a particularly important role in the psychological culture of the head as the leader of the organization, because this competence of the management becomes a “role model” for the employees of the organization. In this article the concept of psychological culture is considered, the level of psychological culture and private types of cultural and psychological behavioral manifestations of heads of diff erent levels of management is estimated. As a result of the study, the relationship between the level of psychological culture of managers and their socio-demographic characteristics is revealed. On the basis of the obtained data, recommendations for group training of managers have been developed. The results of the study can be applied in Russian organizations to implement the competence approach in human resources management as a key in their activities.
Modern Problems Of Hr-Management In Fmcg Retail Chains
Authors: Hrisanova T. , Ashurbekov R.
Published: January 2020

The article discusses the most relevant problems in HR-management of modern retail chains in Russia. Efficient HR-management and coordinated work in all HRM subsystems are the keys to a long-lasting competitiveness in context of the proceeding transfer to a new sustainable development economics. The FMCG-segment is distinguished from many other economical segments in Russia by its high competitiveness and market saturation. Under such circumstances it’s highly important not only to manage the staff , but also to take a proactive approach, which means to diagnose the HRM-system, to identify all the possible problems and to implement the most effi cient HR-technologies. This article shows a wide range of relevant HR-problems in all HRM-subsystems, which may threaten Russian FMCG-retail chains.
Shift Planning Under Delay Uncertainty at Air France: A Vehicle-Scheduling Problem with Outsourcing
Authors: Poullet Julie , Parmentier Axel
Published: January 2020

Airlines must operate many jobs in airports, such as passenger check-in or runway tasks. In airlines’ hubs, airlines generally choose to perform these jobs with their own agents. Shift planning aims at building the sequences of jobs operated by the airline agents and has been widely studied given its impact on operating costs. The impact of delayed flights is generally not taken into account despite the propagation of flight delays along these sequences: If a flight is late, then the agents doing the corresponding jobs are delayed, and may arrive late to their next jobs and delay the corresponding flights. Since delay costs are much higher than the costs of outsourcing jobs, if the agent who is supposed to operate a job is still working elsewhere when the job begins, then airlines tend to outsource the job to their own dedicated team or to a third party. We introduce a stochastic version of the shift-planning problem that takes into account outsourcing costs due to delay. It can be seen as a natural stochastic generalization of the vehicle-scheduling problem in which delayed jobs are outsourced. We propose a column-generation approach to solve it, whose key element is the pricing subproblem algorithm, modeled as a stochastic resource-constrained shortest-path problem. Numerical results on Air France industrial instances show the benefits of using our stochastic version of the shift-planning problem and the efficiency of the solution method. Moving to the stochastic version enables Air France to reduce total operating costs by 3.5%–4.8% on instances with more than 200 jobs, and our algorithm can solve to near optimality instances with up to 400 jobs.
The 2020 Nightingale bicentenary celebrations
Authors: Peate Ian
Published: January 2020

This year marks the Year of the Nurse and Midwife and the Florence Nightingale bicentenary. In this article, Ian Peate discusses the life and work of Nightingale, a continuing inspiration to nurses and midwives everywhere
EVENTOS ADVERSOS E QUEIXAS TÉCNICAS NOTIFICADOS A UM NÚCLEO DE SEGURANÇA DO PACIENTE
Authors: RIBAS MARISTELA ANJOS , ALMEIDA PAULO HENRIQUE RIBEIRO FERNANDES , CHAVES GILMARA ARAÚJO , Lemos Gisele da Silveira
Published: January 2020

Introdução: As notificações de eventos adversos (EA) e queixas técnicas (QT) são importantes ferramentas na garantia da qualidade assistencial. Objetivo: Avaliar as notificações de EA e QT realizadas ao núcleo de segurança do paciente (NSP) de um hospital público de ensino. Materiais e Métodos: Estudo transversal, realizado em um hospital de ensino, referência regional para 26 municípios do Sudoeste da Bahia. Foram coletadas todas as notificações de EA e QT recebidas pelo NSP referentes aos anos 2016 e 2017. A tabulação e análise dos dados foi realizada com auxílio do software EpiData (3.1) e SPSS (25.0). Foi adotado um nível de significância estatística de p < 0.05. Resultados: Observou-se que 91,8% das notificações corresponderam a EA e 8,2% a QT. Pacientes do sexo masculino estiveram mais envolvidos em EA (60,8%). Dentre os EA a lesão por pressão foi o mais notificado (38,2%). A Unidade de Terapia Intensiva e os profissionais de enfermagem foram a unidade e categoria profissional que mais notificaram (37,4 e 61,4%, respectivamente). O tempo gasto entre o incidente ou EA e a sua notificação estiveram estatisticamente associados ao tipo de EA e evolução do paciente. Conclusão: A avaliação das notificações encaminhadas ao NSP no período estudado reforçaram a necessidade de se empreender medidas que melhorem a cultura e os cuidados referentes a segurança do paciente na instituição.
NÓS-OUTRAS. COMPORTAMENTOS E INTERVENCIÓNS TRADUTIVAS UNA REVISIÓN CRÍTICA PERMANENTE.
Authors: Fernández Rodríguez Áurea , Luna Alonso Ana
Published: January 2020

El número monográfico titulado Nós Outras. Comportamentos eintervencións tradutivas nace como un proyecto más del grupo deinvestigación BITRAGA de la Facultade de Filoloxía e Tradución dela Universidade de Vigo. Se trata de la creación de un espacio dedebate sobre Feminismos y Estudios de Traducción e Interpretación(ETI) que quiere ir más allá del campo literario, lugar en elque se han ido desarrollando tradicionalmente estos estudios.
EDITORIAL PATASDEPEIXE: BREVE ANÁLISIS DEL CATÁLOGO Y POLÍTICAS DE EDICIÓN Y TRADUCCIÓN DESDE UNA PERSPECTIVA FEMINISTA.
Authors: Sáez Fernández Laura
Published: January 2020

Este artículo es una aproximación no académica al desarrollo de laeditorial Patasdepeixe en su primera década de actividad. Pretendemosanalizar su catálogo desde el punto de vista formal y de lasautorías, así como encuadrar la editora en el contexto de laindustria editorial en Galicia desde una perspectiva feminista.
LA TRADUCCIÓN HOY EN DÍA: RETRATO DE UNA PROFESIÓN FEMINIZADA. ASPECTOS ÉTICOS Y LABORALES
Authors: Santaemilia José
Published: January 2020

Este artículo aportará datos para un debate sobre la presencia delas mujeres en el mundo de la traducción profesional, sobre todoen la traducción literaria o editorial. Para ello, observaremos quépresencia tienen las mujeres en la universidad y en el mercadolaboral, atendiendo a las diversas disciplinas y ámbitos profesionales.Realizaremos un diagnóstico de la situación de las mujeres enel mundo profesional (prestando atención a la cultura laboralmasculinizada, los estereotipos y expectativas sociales en torno alas mujeres, las cualidades ‘femeninas’, etc.), lo que nos llevará aconcluir que los puestos de trabajo están sexuados en su prácticatotalidad, y que las mujeres sufren habitualmente una doblesegregación (horizontal y vertical) que limita seriamente suparticipación plena en el mundo laboral. A raíz del diagnósticorealizado, pasaremos a valorar las consecuencias que todo ellotiene para las mujeres. Tras repasar algunos indicadores contenidosen diversos informes sobre traducción editorial (2010, 2012,2017), publicados tanto por el Ministerio de Cultura como por laasociación ACE Traductores, que ponen de manifiesto que latraducción es un sector laboral feminizado, reflexionamos (de lamano de autores como Pierre Bourdieu 1998, Richard Anker 1998o Clare Walsh 2001, sobre los estereotipos (positivos y negativos)tradicionalmente asociados a las mujeres, y su concreción en elmundo de la traducción.
POR UNA GEOPOLÍTICA FEMINISTA DE LA TRADUCCIÓN: ESCRITORAS (GALLEGAS) TRADUCIDAS EN EL MERCADO EDITORIAL BRITÁNICO1
Authors: Castro Olga
Published: January 2020

Estudios recientes demuestran una (tímida) apertura a la traducción del mercado editorial británico en los últimos años, especialmente a la narrativa en otros idiomas europeos, gracias sobre todo a la labor de pequeñas editoriales independientes. Tras contextualizar las actuales dinámicas de traducción en Gran Bretaña, este artículo propone analizarlas desde una perspectiva feminista y articular una geopolítica feminista de traducción que potencie flujos de escritoras desde culturas minorizadas y lenguas poco traducidas hacia espacios hegemónicos e hipercentrales como el británico. Persigue con esto dos objetivos. El primero, centrado en el sistema literario meta, consiste en analizar el lugar que ocupa la traducción de escritoras en el mercado editorial británico en el presente, evaluando el impacto de diferentes iniciativas recientes en las redes sociales (como la campaña de twitter #WITMonth y el tumblr “Women in Translation”), blogs y revistas literarias (como Translating Women, LitHub o Words without Borders), premios (como el “Warwick Prize for Women in Translation”) y propuestas editoriales (como el “Year of Publishing Women” en 2018), y pudiendo concluir que el sistema británico está ávido por acercarse a la producción de escritoras extranjeras. El segundo, centrado en el sistema literario gallego como ejemplo paradigmático de cultura minorizada, busca examinar las particularidades de la traducción de escritoras gallegas a inglés en Gran Bretaña, relacionándolas con las dinámicas de exportación en marcha y evaluando su aportación a debates generales sobre la traducción de autoras. En su conjunto, este trabajo pretende servir como estímulo alentador para promover la traducción de escritoras desde lenguas poco traducidas y literaturas minorizadas, avanzando así en una geopolítica feminista de la traducción en el mercado editorial británico.
A critical review of the academic entrepreneurial ecosystem in developing countries: The case of Algeria
Authors: Chohra Mohamed
Published: January 2020

The objective of this article is to conduct a critical review of the literature related to the academic entrepreneurial ecosystems in developing countries in order to highlight the perspectives and to raise new questions in this area of research in these countries. The first part of this study examines the entrepreneurial context and innovation systems to determine the socio-economic and political factors that can have a negative influence on the emergence of an effective academic entrepreneurship in emerging economies. The second part explores the phenomenon of the academic entrepreneurial ecosystem in Algeria to identify the main issues and challenges that may have to be taken into consideration while promoting academic entrepreneurship in Algeria. In this study, we focus on the experience of the pharmaceutical industry as a successful model to develop effective approaches for commercializing the results of the academic research. The article also attempts to define the roles of three main actors, namely, the state, academic institutions and industry.
La danza butoh: Ética y estética del cuerpo volcado hacia afuera
Authors: Gómez Cifuentes Rebeca
Published: January 2020

Desde la primera filosofía se nos ha hecho creer que el sentido de la vida se halla fuera de nosotros. No tenemos cuerpo, sino que somos cuerpo y es en él donde se debe rastrear la memoria colectiva del ser humano. Debemos retornar al propio cuerpo dejando que exprese sus sueños y su locura. Solo ante el arte el hombre trasciende, pues conecta emoción y cognición convirtiendo el cuerpo que sufre en terreno privilegiado para la reflexión y la representación de nuestra propia identidad individual y social. La danza no puede ser reducida al mero esfuerzo físico o al cuerpo visible, debe ser entendida como punto de partida, como eudaimonía. En el butoh el cuerpo se asoma a sus abismos para renacer cada vez que uno danza. Según Heidegger, la salvación, si existe, solo puede estar dentro de nosotros. Enterrémonos con los pies, para volar con los brazos.
Balletvalemas: Refuerzo para la rehabilitación de niños con necesidades motoras especiales a través de la danza clásica adaptada y el desarrollo de una ayuda técnica
Authors: Parra Porras Alicia , Morán Pascual Patricia , Mortes Roselló Esther
Published: January 2020

Una sociedad inclusiva se basa en el desarrollo de los derechos humanos que promueven la justicia social y la igualdad de oportunidades. Ergo, ¿por qué los niños y adolescentes con dificultades motoras, dolor o con cualquier necesidad especial no pueden aficionarse a un deporte o a una disciplina artística como el resto de sus iguales sin discapacidad? La fisioterapia, la educación somática y la danza clásica, junto con la ingenieria, persiguen en este proyecto lograr el control del movimiento a través de un enfoque holístico, donde cuerpo y mente no están separados el uno del otro. ¿Por qué no adaptar clases de danza clásica a niños con necesidades especiales y ofrecerles nuevas sensaciones de movimieto gracias a los ejercicios y a la ayuda técnica desarrollada?