bluuebunny/crossref_metadata_2025 huggingface dataset:
Detection of Serum-Specific IgE by Fluoro-Enzyme Immunoassay for Diagnosing Type I Hypersensitivity Reactions to Penicillins
Authors: Ariza Adriana , Mayorga Cristobalina , Bogas Gádor , Gaeta Francesco , Salas María , Valluzzi Rocco L. , Labella Marina , Pérez-Sánchez Natalia , Caruso Cristiano , Molina Ana , Fernández Tahia D. , Torres María José , Romano Antonino
Published: June 2022

Diagnosis of type I hypersensitivity reactions (IgE-mediated reactions) to penicillins is based on clinical history, skin tests (STs), and drug provocation tests (DPTs). Among in vitro complementary tests, the fluoro-enzyme immunoassay (FEIA) ImmunoCAP® (Thermo-Fisher, Waltham, MA, USA) is the most widely used commercial method for detecting drug-specific IgE (sIgE). In this study, we aimed to analyze the utility of ImmunoCAP® for detecting sIgE to penicillin G (PG) and amoxicillin (AX) in patients with confirmed penicillin allergy. The study includes 139 and 250 patients evaluated in Spain and Italy, respectively. All had experienced type I hypersensitivity reactions to penicillins confirmed by positive STs. Additionally, selective or cross-reactive reactions were confirmed by DPTs in a subgroup of patients for further analysis. Positive ImmunoCAP® results were 39.6% for PG and/or AX in Spanish subjects and 52.4% in Italian subjects. When only PG or AX sIgE where analyzed, the percentages were 15.1% and 30.4%, respectively, in Spanish patients; and 38.9% and 46% in Italian ones. The analysis of positive STs showed a statistically significant higher percentage of positive STs to PG determinants in Italian patients. False-positive results to PG (16%) were detected in selective AX patients with confirmed PG tolerance. Low and variable sensitivity values observed in a well-defined population with confirmed allergy diagnosis, as well as false-positive results to PG, suggest that ImmunoCAP® is a diagnostic tool with relevant limitations in the evaluation of subjects with type I hypersensitivity reactions to penicillins.
An Eleven-microRNA Signature Related to Tumor-Associated Macrophages Predicts Prognosis of Breast Cancer
Authors: Jayasingam Sharmilla Devi , Citartan Marimuthu , Mat Zin Anani Aila , Rozhdestvensky Timofey S. , Tang Thean-Hock , Ch’ng Ewe Seng
Published: June 2022

The dysregulation of microRNAs (miRNAs) has been known to play important roles in tumor development and progression. However, the understanding of the involvement of miRNAs in regulating tumor-associated macrophages (TAMs) and how these TAM-related miRNAs (TRMs) modulate cancer progression is still in its infancy. This study aims to explore the prognostic value of TRMs in breast cancer via the construction of a novel TRM signature. Potential TRMs were identified from the literature, and their prognostic value was evaluated using 1063 cases in The Cancer Genome Atlas Breast Cancer database. The TRM signature was further validated in the external Gene Expression Omnibus GSE22220 dataset. Gene sets enrichment analyses were performed to gain insight into the biological functions of this TRM signature. An eleven-TRM signature consisting of mir-21, mir-24-2, mir-125a, mir-221, mir-22, mir-501, mir-365b, mir-660, mir-146a, let-7b and mir-31 was constructed. This signature significantly differentiated the high-risk group from the low-risk in terms of overall survival (OS)/ distant-relapse free survival (DRFS) (p value < 0.001). The prognostic value of the signature was further enhanced by incorporating other independent prognostic factors in a nomogram-based prediction model, yielding the highest AUC of 0.79 (95% CI: 0.72–0.86) at 5-year OS. Enrichment analyses confirmed that the differentially expressed genes were mainly involved in immune-related pathways such as adaptive immune response, humoral immune response and Th1 and Th2 cell differentiation. This eleven-TRM signature has great potential as a prognostic factor for breast cancer patients besides unravelling the dysregulated immune pathways in high-risk breast cancer.
Fine Mapping and Functional Analysis of Major Regulatory Genes of Soluble Solids Content in Wax Gourd (Benincasa hispida)
Authors: Wu Wenting , Wang Peng , Huang Xiaochun , Su Liwen , Lv Haixuan , Gou Jiquan , Cheng Zhikui , Ma Lianlian , Yu Wenjin , Liu Zhengguo
Published: June 2022

Soluble solids content (SSC) is an important quality trait of wax gourd, but reports about its regulatory genes are scarce. In this study, the SSC regulatory gene BhSSC2.1 in wax gourd was mined via quantitative trait locus (QTL) mapping based on high-density genetic mapping containing 12 linkage groups (LG) and bulked segregant analysis (BSA)-seq. QTL mapping and BSA-seq revealed for the first time that the SSC QTL (107.658–108.176 cM) of wax gourd was on Chr2 (LG2). The interpretable phenotypic variation rate and maximum LOD were 16.033% and 6.454, respectively. The QTL interval contained 13 genes. Real-time fluorescence quantitative expression analysis, functional annotation, and sequence analysis suggested that Bch02G016960, named BhSSC2.1, was a candidate regulatory gene of the SSC in wax gourd. Functional annotation of this gene showed that it codes for a NADP-dependent malic enzyme. According to BhSSC2.1 sequence variation, an InDel marker was developed for molecular marker-assisted breeding of wax gourd. This study will lay the foundation for future studies regarding breeding and understanding genetic mechanisms of wax gourd.
Human IL12p80 Promotes Murine Oligodendrocyte Differentiation to Repair Nerve Injury
Authors: Chung Yu-Fen , Chen Jong-Hang , Li Ching-Wen , Hsu Hui-Yu , Chen Ya-Ping , Wang Chiao-Chan , Chiu Ing-Ming
Published: June 2022

Nerve injury of the central nervous system and the peripheral nervous system still poses a major challenge in modern clinics. Understanding the roles of neurotrophic factors and their molecular mechanisms on neuro-regeneration will not only benefit patients with neural damage but could potentially treat neurodegenerative disorders, such as amyotrophic lateral sclerosis. In this study, we showed that human IL12 p40-p40 homodimer (hIL12p80) within PLA and PLGA conduits improved sciatic nerve regeneration in mice. As such, the group of conduits with NSCs and hIL12p80 (CNI) showed the best recovery among the groups in the sciatic functional index (SFI), compound muscle action potential (CMAP), and Rotarod performance analyses. In addition, the CNI group had a faster recovery and outperformed the other groups in SFI and Rotarod performance tests beginning in the fourth week post-surgery. Immunohistochemistry showed that the CNI group increased the diameter of the newly regenerated nerve by two-fold (p < 0.01). In vitro studies showed that hIL12p80 stimulated differentiation of mouse NSCs to oligodendrocyte lineages through phosphorylation of Stat3 at Y705 and S727. Furthermore, implantation using PLGA conduits (C2.0 and C2.1) showed better recovery in the Rotarod test and CMAP than using PLA conduits in FVB mice. In B6 mice, the group with C2.1 + NSCs + hIL12p80 (C2.1NI) not only promoted sciatic functional recovery but also reduced the rate of experimental autotomy. These results suggested that hIL12p80, combined with NSCs, enhanced the functional recovery and accelerated the regeneration of damaged nerves in the sciatic nerve injury mice. Our findings could further shed light on IL12′s application not only in damaged nerves but also in rectifying the oligodendrocytes’ defects in neurodegenerative diseases, such as amyotrophic lateral sclerosis and multiple sclerosis.
Collagen Mimetic Peptides Promote Adherence and Migration of ARPE-19 Cells While Reducing Inflammatory and Oxidative Stress
Authors: Ribeiro Marcio , Pasini Silvia , Baratta Robert O. , Del Buono Brian J. , Schlumpf Eric , Calkins David J.
Published: June 2022

Epithelial cells of multiple types produce and interact with the extracellular matrix to maintain structural integrity and promote healthy function within diverse endogenous tissues. Collagen is a critical component of the matrix, and challenges to collagen’s stability in aging, disease, and injury influence survival of adherent epithelial cells. The retinal pigment epithelium (RPE) is important for maintaining proper function of the light-sensitive photoreceptors in the neural retina, in part through synergy with the collagen-rich Bruch’s membrane that promotes RPE adherence. Degradation of Bruch’s is associated with RPE degeneration, which is implicated early in age-related macular degeneration, a leading cause of irreversible vision loss worldwide. Collagen mimetic peptides (CMPs) effectively repair damage to collagen helices, which are present in all collagens. Our previous work indicates that in doing so, CMPs promote survival and integrity of affected cells and tissues in models of ocular injury and disease, including wounding of corneal epithelial cells. Here, we show that CMPs increase adherence and migration of the ARPE-19 line of human RPE cells challenged by digestion of their collagen substrate. Application of CMPs also reduced both ARPE-19 secretion of pro-inflammatory cytokines (interleukins 6 and 8) and production of reactive oxygen species. Taken together, these results suggest that repairing collagen damaged by aging or other pathogenic processes in the posterior eye could improve RPE adherence and survival and, in doing so, reduce the inflammatory and oxidative stress that perpetuates the cycle of destruction at the root of age-related diseases of the outer retina.
Distinct Transcriptional Profile of PDZ Genes after Activation of Human Macrophages and Dendritic Cells
Authors: Rosas-García Jorge , Ramón-Luing Lucero A. , Bobadilla Karen , Meraz-Ríos Marco Antonio , Sevilla-Reyes Edgar E. , Santos-Mendoza Teresa
Published: June 2022

The PDZ (PSD95, Dlg and ZO-1) genes encode proteins that primarily function as scaffolds of diverse signaling pathways. To date, 153 PDZ genes have been identified in the human genome, most of which have multiple protein isoforms widely studied in epithelial and neural cells. However, their expression and function in immune cells have been poorly studied. Herein, we aimed to assess the transcriptional profiles of 83 PDZ genes in human macrophages (Mɸ) and dendritic cells (DCs) and changes in their relative expression during cell PRR stimulation. Significantly distinct PDZ gene transcriptional profiles were identified under different stimulation conditions. Furthermore, a distinct PDZ gene transcriptional signature was found in Mɸ and DCs under the same phagocytic stimuli. Notably, more than 40 PDZ genes had significant changes in expression, with potentially relevant functions in antigen-presenting cells (APCs). Given that several PDZ proteins are targeted by viral products, our results support that many of these proteins might be viral targets in APCs as part of evasion mechanisms. Our results suggest a distinct requirement for PDZ scaffolds in Mɸ and DCs signaling pathways activation. More assessments on the functions of PDZ proteins in APCs and their role in immune evasion mechanisms are needed.
Research Advances in Age-Related Macular Degeneration
Authors: Abdolrahimzadeh Solmaz
Published: June 2022

The first descriptions of the condition now known as age-related macular degeneration (AMD) appeared in 1852; however, it is only since the 1970s that our knowledge on AMD has substantially increased [...]
Treatment and Prognosis of Vogt–Koyanagi–Harada Disease: Real-Life Experience in Long-Term Follow-Up
Authors: Accorinti Massimo , Saturno Maria Carmela , Iannetti Ludovico , Manni Priscilla , Mastromarino Davide , Pirraglia Maria Pia
Published: June 2022

Background: Vogt–Koyanagi–Harada (VKH) disease is a form of uveitis that is rare in Western countries. The aim of this study was to report on the long-term real-life treatment and prognosis of VKH in Italy. Methods: The clinical features, complications, and final visual acuity were retrospectively evaluated in 38 patients with VKH (mean follow-up: 120 months) globally, according to oral or intravenous corticosteroid treatment at onset and subsequent immunosuppressive therapy. Results: The mean final visual acuity was 0.13 ± 0.4 logMAR, which was a significant increase from the baseline (p < 0.0001). The patients who received intravenous rather than oral corticosteroids relapsed less (p = 0.026), with fewer relapses/patient/month of follow-up (p < 0.0001), and showed less frequent sunset glow fundus (33.3% versus 55%) and more relapse-free cases after induction therapy (p = 0.007). Delayed immunosuppressive therapy (median: 180 days from the onset of symptoms) reduced the rate of sunset glow fundus. The onset of sunset glow fundus was associated with a worse final visual acuity (p = 0.006). Conclusion: The long-term prognosis of VKH is quite good. Intravenous corticosteroids given at the onset of VKH are more effective than oral corticosteroids. Even if it is not given immediately after symptoms onset, immunosuppressive therapy is able to reduce the incidence of sunset glow fundus and to improve the final visual prognosis.
Incidence, Outcome, and Risk Factors of Cardiovascular Surgery-Associated Disseminated Intravascular Coagulation: A Single-Center Retrospective Study
Authors: Yasuda Norihisa , Goto Koji , Kuribayashi Yoshihide , Ohchi Yoshifumi , Kitano Takaaki
Published: June 2022

Cardiovascular surgery is highly invasive, with a risk of postoperative coagulopathy due to various factors such as bleeding. Coagulopathy can progress to disseminated intravascular coagulation (DIC), which complicates various clinical conditions. However, no study to date has reported on DIC associated with cardiovascular surgery. Therefore, we investigated retrospectively the incidence, outcome, and risk factors of cardiovascular surgery-associated DIC in our institute. All patients who underwent cardiovascular surgery and were admitted to our intensive care unit between January 2016 and December 2017 were included in this study. The Japanese Association for Acute Medicine (JAAM) DIC score was calculated using our institute’s database at the following time points: preoperatively, postoperative day 1 (POD1), POD3, and POD7. Data regarding surgery, 90-day mortality, and risk factors of DIC were also collected and analyzed by multiple regression. In total, 553 patients were considered eligible for analysis. Median age of eligible patients was 72 years, with a 90-day mortality rate of 1.4%. Patients with DIC at POD7 had higher Sequential Organ Failure Assessment (SOFA) score, preoperative JAAM DIC scores, and a longer anesthesia time than those without DIC. Female sex, preoperative DIC score, and anesthesia time were found to be risk factors for DIC.
Risk Factors for Recurrence of Borderline Ovarian Tumours after Conservative Surgery and Impact on Fertility: A Multicentre Study by the Francogyn Group
Authors: Ozenne Adele , De Berti Marion , Body Gilles , Carcopino Xavier , Graesslin Olivier , Kerbage Yohan , Akladios Cherif , Huchon Cyrille , Bricou Alexandre , Mimoun Camille , Raimond Emilie , Ouldamer Lobna
Published: June 2022

Introduction: Borderline ovarian tumours (BOT) represent 10–20% of epithelial tumours of the ovary. Although their prognosis is excellent, the recurrence rate can be as high as 30%, and recurrence in the infiltrative form accounts for 3% to 5% of recurrences. Affecting, in one third of cases, women of childbearing age, the surgical strategy with ovarian conservation is now recommended despite a significant risk of recurrence. Few studies have focused exclusively on patients who have received ovarian conservative treatment in an attempt to identify factors predictive of recurrence and the impact on fertility. The objective of this study was to identify the risk factors for recurrence of BOT after conservative treatment and the impact on fertility. Material and methods: This was a retrospective, multicentre study of women who received conservative surgery for BOT between February 1997 and September 2020. We divided the patients into two groups, the “R group” with recurrence and the “NR group” without recurrence. Results: Of 175 patients included, 35 had a recurrence (R group, 20%) and 140 had no recurrence (NR group, 80%). With a mean follow-up of 30 months (IQ 8–62.5), the overall recurrence rate was 20%. Recurrence was BOT in 17.7% (31/175) and invasive in 2.3% (4/175). The mean time to recurrence was 29.5 months (IQ 16.5–52.5). Initial complete peritoneal staging (ICPS) was performed in 42.5% of patients (n = 75). In multivariate analysis, age at diagnosis, nulliparity, advanced FIGO stage, the presence of peritoneal implants, and the presence of a micropapillary component for serous tumours were factors influencing the occurrence of recurrence. The post-surgery fertility rate was 67%. Conclusion: This multicentre study is to date one of the largest studies analysing the risk factors for recurrence of BOT after conservative surgery. Five risk factors were found: age at diagnosis, nulliparity, advanced FIGO stage, the presence of implants, and a micropapillary component. Only 25% of the patients with recurrence underwent ICPS. These results reinforce the interest of initial peritoneal staging to avoid ignoring an advanced tumour stage.
Machine-Learning Algorithm for Predicting Fatty Liver Disease in a Taiwanese Population
Authors: Chen Yang-Yuan , Lin Chun-Yu , Yen Hsu-Heng , Su Pei-Yuan , Zeng Ya-Huei , Huang Siou-Ping , Liu I-Ling
Published: June 2022

The rising incidence of fatty liver disease (FLD) poses a health challenge, and is expected to be the leading global cause of liver-related morbidity and mortality in the near future. Early case identification is crucial for disease intervention. A retrospective cross-sectional study was performed on 31,930 Taiwanese subjects (25,544 training and 6386 testing sets) who had received health check-ups and abdominal ultrasounds in Changhua Christian Hospital from January 2009 to January 2019. Clinical and laboratory factors were included for analysis by different machine-learning algorithms. In addition, the performance of the machine-learning algorithms was compared with that of the fatty liver index (FLI). Totally, 6658/25,544 (26.1%) and 1647/6386 (25.8%) subjects had moderate-to-severe liver disease in the training and testing sets, respectively. Five machine-learning models were examined and demonstrated exemplary performance in predicting FLD. Among these models, the xgBoost model revealed the highest area under the receiver operating characteristic (AUROC) (0.882), accuracy (0.833), F1 score (0.829), sensitivity (0.833), and specificity (0.683) compared with those of neural network, logistic regression, random forest, and support vector machine-learning models. The xgBoost, neural network, and logistic regression models had a significantly higher AUROC than that of FLI. Body mass index was the most important feature to predict FLD according to the feature ranking scores. The xgBoost model had the best overall prediction ability for diagnosing FLD in our study. Machine-learning algorithms provide considerable benefits for screening candidates with FLD.
Cardiorenal Crosstalk in Patients with Heart Failure
Authors: Schukraft Sara , Hullin Roger
Published: June 2022

Worsening renal function is associated with poor outcomes in heart failure and often accompanies the initiation and up-titration of guidelines-directed heart failure therapy. This narrative review summarizes current evidence on immediate and long-term effects of pharmacological or device-based treatment in heart failure patients with reduced or preserved left ventricular ejection fraction.
Historical Agricultural Landforms—Central European Bio-Cultural Heritage Worthy of Attention
Authors: Zacharová Johana , Riezner Jiří , Elznicová Jitka , Machová Iva , Kubát Karel , Holcová Diana , Holec Michal , Pacina Jan , Štojdl Jiří , Grygar Tomáš Matys
Published: June 2022

Knowledge about past agricultural land management can bring solutions for future needs. One undervalued historical type of historical rural landscape in temperate Europe is termed plužiny. It consists of individual historical agricultural landforms framed by linear woody vegetation. Our multidisciplinary research quantified the distribution of plužiny in Czechia, utilizing archive materials, geographic information systems, and field surveys for verification. Several case studies give merit to the societal relevance of plužiny and justification for their protection and inclusion in landscape planning. We have assessed the contribution of plužiny to secondary geodiversity by describing the landforms morphometrically, using geophysical imaging of their inner structure, and assessing the possible downslope erosive segregation of soil particles. The results of these analyses prove the positive effect of these landscape features on secondary geodiversity and biodiversity at the species level through the process of induced landscape diversification. The results also document management changes during the last 170 years and provide a basis for assessing their present-day endangerment. Although plužiny are less known compared to bocage landscapes of Western Europe, they are similarly valuable. Landscape managers should better recognize the ecological, cultural, and aesthetic values of plužiny as historical agricultural landforms and protect them as a bio-cultural heritage.
Immune Responses to Some Viral Infections That Have a High Evolutionary Potential—A Case Report with Literature Review
Authors: Sur Maria Lucia , Moldovan Bogdan-Stefan , Mocanu Diana , Samasca Gabriel , Lupan Iulia , Armat Ionel , Harabagiu Marin , Sur Genel , Lazar Calin
Published: June 2022

Viral infections are a key issue in modern medicine. SARS-CoV-2 infection confirms that we are not sufficiently prepared for these unforeseen infections. The COVID-19 pandemic has cultivated a great sense of fear and distrust in patients. If viral infections, in this case, SARS-CoV-2, overlap with another infection, the symptoms are prolonged and worsened, and complications may occur. Starting from an objective clinical finding of a patient they had in follow-up and treatment, the authors present the problems of the diseases the patient suffered from. These are described as reviews so that readers can get an idea of the clinical methods of expression and the therapeutic possibilities. Therefore, this article describes Lyme disease and post-treatment Lyme disease syndrome, SARS-CoV-2 infection, and multisystem inflammatory syndrome in children (MISC-C), as the patient suffered from an incomplete form of Kawasaki disease. During the treatment for Lyme disease, the patient also contracted the influenza type A virus. Although any of these diseases could have the potential for serious evolution, our patient still went through these infections relatively well. This can be explained by the fact that the patient had a slow immune response to the aforementioned infections, which allowed him to survive these diseases relatively easily, unlike other individuals who have an exaggerated immune response or who suffer from serious immune involvement, e.g., hepatitis B with a fulminant response. The case was presented chronologically, but at the same time, all particular infection manifestations were accurately described. For these reasons, the article is presented in the form of a review, exemplified by the case itself. Of the 52 cases of MISC-C found in the Pediatrics Clinic II of Cluj-Napoca, we present the case of a male patient who presented with Lyme disease, post-treatment Lyme disease syndrome, Kawasaki disease, and MISC-C incomplete form.
Insight into the Roles of Proline-Rich Extensin-like Receptor Protein Kinases of Bread Wheat (Triticum aestivum L.)
Authors: Shumayla  , Mendu Venugopal , Singh Kashmir , Upadhyay Santosh Kumar
Published: June 2022

Proline-rich extensin-like receptor protein kinases (PERKs) are known for their roles in the developmental processes and stress responses of many plants. We have identified 30 TaPERK genes in the genome of T. aestivum, exploring their evolutionary and syntenic relationship and analyzing their gene and protein structures, various cis-regulatory elements, expression profiling, and interacting miRNAs. The TaPERK genes formed 12 homeologous groups and clustered into four phylogenetic clades. All the proteins exhibited a typical domain organization of PERK and consisted of conserved proline residue repeats and serine-proline and proline-serine repeats. Further, the tyrosine-x-tyrosine (YXY) motif was also found conserved in thirteen TaPERKs. The cis-regulatory elements and expression profiling under tissue developmental stages suggested their role in plant growth processes. Further, the differential expression of certain TaPERK genes under biotic and abiotic stress conditions suggested their involvement in defense responses as well. The interaction of TaPERK genes with different miRNAs further strengthened evidence for their diverse biological roles. In this study, a comprehensive analysis of obtained TaPERK genes was performed, enriching our knowledge of TaPERK genes and providing a foundation for further possible functional analyses in future studies.
Risk of Cardiovascular Disease in Apnoeic Individuals: Role of Comorbid Insomnia Disorder
Authors: Hein Matthieu , Wacquier Benjamin , Lanquart Jean-Pol , Loas Gwenolé
Published: June 2022

Given the limited data available, the aim of this study was to examine the 10-year cardiovascular disease (CVD) risk associated with comorbid insomnia disorder and its specific subtypes in apnoeic individuals. Data from 1104 apnoeic individuals recruited from the database of the Erasme Hospital Sleep Laboratory were analysed. Only apnoeic individuals with a Framingham Risk Score ≥10% were included in the group at moderate-to-high 10-year CVD risk. Logistic regression analyses were conducted to examine the risk of 10-year CVD risk associated with comorbid insomnia disorder and its specific subtypes in apnoeic individuals. Moderate-to-high 10-year CVD risk was present in 59.6% of the apnoeic individuals in our sample. After adjustment for the main confounding factors, multivariate logistic regression analyses revealed that comorbid insomnia disorder and, more particularly, its subtype with short sleep duration were significantly associated with moderate-to-high 10-year CVD risk in apnoeic individuals. In this study, we demonstrate that comorbid insomnia disorder and, more specifically, its subtype with short sleep duration appear to have a negative cumulative effect on 10-year CVD risk in apnoeic individuals, which justifies more systematic research and adequate therapeutic management of this disorder to allow for better cardiovascular disease prevention in this particular subpopulation.
Role of Obesity in Inflammation and Remodeling of Asthmatic Airway
Authors: Shailesh Harshita , Janahi Ibrahim A.
Published: June 2022

Obesity is considered as an important risk factor for the onset of asthma and plays a key role in enhancing the disease’s severity. Obese asthmatic individuals represent a distinct phenotype of asthma that is associated with additional symptoms, more severe exacerbation, decreased response to standard medication, and poor quality of life. Obesity impairs the function of the lung airway in asthmatic individuals, leading to increased inflammation and severe remodeling of the bronchus; however, the molecular events that trigger such changes are not completely understood. In this manuscript, we review the current findings from studies that focused on understanding the role of obesity in modulating the functions of airway cells, including lung immune cells, epithelial cells, smooth muscle cells, and fibroblasts, leading to airway inflammation and remodeling. Finally, the review sheds light on the current knowledge of different therapeutic approaches for treating obese asthmatic individuals. Given the fact that the prevalence of asthma and obesity has been increasing rapidly in recent years, it is necessary to understand the molecular mechanisms that play a role in the disease pathophysiology of obese asthmatic individuals for developing novel therapies.
A Brake System Coefficient of Friction Estimation Using 3D Friction Maps
Authors: Varriale Francesco , Candeo Stefano , Riva Gabriele , Wahlström Jens , Lyu Yezhe
Published: June 2022

The coefficient of friction (COF) is one of the core factors in the evaluation of brake system performance. It is challenging to predict the COF, since it is strongly influenced by several parameters such as contact pressure (p), slip rate (v) and temperature (T) that depend on the driving conditions. There is a need for better models to describe how the brake friction varies under different driving conditions. The purpose of this research is to study the possibility of using 3D friction pvT-maps to estimate the COF of a disc brake system under different driving conditions. The 3D friction pvT-maps are created by filtering results of material tests conducted in a mini-dyno inertia bench. The COF measured under different driving cycles in an inertia dyno bench with the full brake system are compared with the COF estimated by the friction maps coming from the reduced scale dyno bench to investigate the validity of the simulation approach. This study shows that mini dyno bench is suitable to obtain a tribological characterization of the friction pad–disc rotor contact pair and is able to replace the full inertia dyno bench to investigate the brake system performance.
Super-Hard DLC Coatings as an Alternative to Polycrystalline Diamond for Cutting Tools: Predictive Analysis of Aluminium Alloy Surface Quality
Authors: Ramírez Giselle , Gonzalez Castro Jose M. , Orrit-Prat Jordi , Bonet Raül , Cuadrado Nuria , Vilaseca Montserrat , Carreras Lluís , Caro Jaume
Published: June 2022

The use of lightweight materials, such as ultra-high-strength aluminium alloys, is in high demand in the automotive and aerospace industries where weight savings are critical. The tool materials used for high-speed cutting of these aluminium alloys are subjected to severe conditions that promote premature failure of cutting tools. The application of polycrystalline diamond (PCD) coatings provides cutting tools with increased mechanical and thermal fatigue resistance and improved tribological performance. Despite these good properties, their high cost remains a major limitation in this sector. Super-hard Diamond-Like Carbon (DLC) coatings offer a technologically and economically feasible alternative to PCD-coated tools for cutting and machining non-ferrous materials. In this paper, the machining performance of coated and un-coated hard metal inserts in the turning of 7075 aluminium alloy has been explored. The surface quality of machined parts, the cutting tool wear resistance and the vibrations generated during turning of un-coated, PCD and super-hard thin DLC coatings on tungsten carbide inserts were compared. The results obtained demonstrate that DLC coatings are a potentially interesting alternative to PCD coatings for machining ultra-high-strength aluminium alloys, where surface component finish is a key factor.
Characterization of Carbon Nanostructures by Photoelectron Spectroscopies
Authors: Speranza Giorgio
Published: June 2022

Recently, the scientific community experienced two revolutionary events. The first was the synthesis of single-layer graphene, which boosted research in many different areas. The second was the advent of quantum technologies with the promise to become pervasive in several aspects of everyday life. In this respect, diamonds and nanodiamonds are among the most promising materials to develop quantum devices. Graphene and nanodiamonds can be coupled with other carbon nanostructures to enhance specific properties or be properly functionalized to tune their quantum response. This contribution briefly explores photoelectron spectroscopies and, in particular, X-ray photoelectron spectroscopy (XPS) and then turns to the present applications of this technique for characterizing carbon nanomaterials. XPS is a qualitative and quantitative chemical analysis technique. It is surface-sensitive due to its limited sampling depth, which confines the analysis only to the outer few top-layers of the material surface. This enables researchers to understand the surface composition of the sample and how the chemistry influences its interaction with the environment. Although the chemical analysis remains the main information provided by XPS, modern instruments couple this information with spatial resolution and mapping or with the possibility to analyze the material in operando conditions at nearly atmospheric pressures. Examples of the application of photoelectron spectroscopies to the characterization of carbon nanostructures will be reviewed to present the potentialities of these techniques.
Degradation Law and Service Life Prediction Model of Tunnel Lining Concrete Suffered Combined Effects of Sulfate Attack and Drying–Wetting Cycles
Authors: Lu Feng , Wang Haiyan , Wang Lichuan , Zhao Kai , Zhang Junru
Published: June 2022

The present study explored the degradation law and service life prediction of tunnel lining concrete with different mineral admixtures under coupled actions of sulfate attack (SA) and drying–wetting (DW) cycles. The deterioration resistance coefficient (DRC) of compressive strength and influence coefficients of sulfate concentration, mineral admixture content, water/binder (w/b) ratio, and curing regime on DRC were studied. After that, a new service life prediction model based on damage mechanics was developed and analyzed. Results show that, by increasing the DW cycles, the DRC first increases and then decreases. DRCs of Ordinary Portland cement (OPC), fly ash (FA), and ground granulated blast-furnace slag (GGBS) concrete linearly decrease with the increase of sulfate concentration, while the silica fume (SF) concrete displays a two-stage process; by increasing the admixture content, the DRCs of FA and GGBS concrete exhibit two distinct stages, while the SF concrete depicts a three-stage process; increasing the w/b ratio linearly decreases the DRC; the DRC of curing regime was sequenced as standard curing (SC) > fog curing (FC) > water curing (WC) > same condition curing (SCC). Based on the experimental results, the service life prediction model is applied and validated. The validation results show that the proposed model can accurately predict the lifetime of concrete with different mix proportions. Furthermore, it is found that the mineral admixture can effectively improve the lifetime of concrete, and the composite mineral admixture is more effective than a single mineral admixture in improving the lifetime of concrete.
Effect of TiC on Microstructure and Properties of Wear-Resistant Mo2FeB2 Claddings
Authors: Sun Yiqun , Sun Junsheng , Jin Jun , Xu Hu
Published: June 2022

Alloy blocks with different TiC content were designed, and Mo2FeB2 cermets were prepared by carbon arc surfacing process. The interaction law of TiC content and the microstructure, phase, composition, hardness and wear resistance of the cladding were studied in detail by the combination of experiment and theoretical analysis. On the other hand, the phase transition process of the weldpool is theoretically analyzed by thermodynamic calculation method. XRD test results show that in addition to Mo2FeB2 synthesized in situ, the cladding also forms phases such as TiC, CrB, MoB and Fe-Cr. The number of Mo2FeB2 hard phases gradually increases when TiC content varies from 0% to 15%. The average microhardness of the cladding with 0%, 5%, 10%, and 15% TiC was 992 HV0.5, 1035 HV0.5, 1018 HV0.5 and 689 HV0.5, respectively, with 5% TiC being the largest. Moreover, the cladding with 5% TiC content has excellent wear resistance, which is 14.6 times that of the substrate.
Self-Oscillating Liquid Gating Membranes with Periodic Gas Transport
Authors: Xu Xue , Liu Jing , Cao Min , Zhang Jian , Huang Xinlu , Hou Xu
Published: June 2022

Liquid gating membranes with molecular-level smooth liquid lining layers break through the limitations of traditional porous membrane materials in gas transport control. Owing to the stable, self-healing, and reconfigurable properties, liquid gating membranes have shown wide application prospects in microfluidics, intelligent valves, chemical reactions, and beyond. Here, we develop a periodic gas transport control system based on the self-oscillating liquid gating membrane. Under continuous gas injection, the gas–liquid interface is reversibly deformed, enabling self-oscillating behavior for discontinuous and periodic gas transport without the need for any complex external changes to the original system. Meanwhile, our experimental analysis reveals that the periodic time and periodic gas release in the system can be regulated. Based on the cycle stability of the system, we further demonstrate the controllability of the system for periodic droplet manipulation in microfluidics. Looking forward, it will offer new opportunities for various applications, such as pneumatic robots, gas-involved chemical reactions, droplet microfluidics, and beyond.
Novel Perspective of Cardiovascular Diseases: Volume-Regulatory Anion Channels in the Cell Membrane
Authors: Hou Liming , Liu Yan , Sun Chao , Xu Rong , Cao Guihua , Wang Xiaoming
Published: June 2022

Cardiovascular diseases (CVDs) are the leading cause of morbidity and mortality worldwide. Although there are established mechanisms and preventions for CVDs, they are not totally elucidative and effective. Emerging evidence suggests that the dysregulation of ion channels in the cell membranes underpins the dysfunction of the cardiovascular system. To date, a variety of cation channels have been widely recognized as important targets for the treatment of CVDs. As a critical component of the anion channels, the volume-regulated anion channel (VRAC) is involved in a series of cell functions by the volume regulation and maintenance of membrane homeostasis. It has been confirmed to play crucial roles in cell action potential generation, cell proliferation, differentiation and apoptosis, and the VRAC appears to be a major participant in metabolic processes during CVDs. This review summarizes the current evidence and progress concerning the VRAC, to determine the future directions and challenges for CVDs for both preventive and therapeutic purposes.
Challenges, Opportunities and Future Directions of Membrane Technology for Natural Gas Purification: A Critical Review
Authors: Imtiaz Aniqa , Othman Mohd Hafiz Dzarfan , Jilani Asim , Khan Imran Ullah , Kamaludin Roziana , Iqbal Javed , Al-Sehemi Abdullah G.
Published: June 2022

Natural gas is an important and fast-growing energy resource in the world and its purification is important in order to reduce environmental hazards and to meet the required quality standards set down by notable pipeline transmission, as well as distribution companies. Therefore, membrane technology has received great attention as it is considered an attractive option for the purification of natural gas in order to remove impurities such as carbon dioxide (CO2) and hydrogen sulphide (H2S) to meet the usage and transportation requirements. It is also recognized as an appealing alternative to other natural gas purification technologies such as adsorption and cryogenic processes due to its low cost, low energy requirement, easy membrane fabrication process and less requirement for supervision. During the past few decades, membrane-based gas separation technology employing hollow fibers (HF) has emerged as a leading technology and underwent rapid growth. Moreover, hollow fiber (HF) membranes have many advantages including high specific surface area, fewer requirements for maintenance and pre-treatment. However, applications of hollow fiber membranes are sometimes restricted by problems related to their low tensile strength as they are likely to get damaged in high-pressure applications. In this context, braid reinforced hollow fiber membranes offer a solution to this problem and can enhance the mechanical strength and lifespan of hollow fiber membranes. The present review includes a discussion about different materials used to fabricate gas separation membranes such as inorganic, organic and mixed matrix membranes (MMM). This review also includes a discussion about braid reinforced hollow fiber (BRHF) membranes and their ability to be used in natural gas purification as they can tackle high feed pressure and aggressive feeds without getting damaged or broken. A BRHF membrane possesses high tensile strength as compared to a self-supported membrane and if there is good interfacial bonding between the braid and the separation layer, high tensile strength, i.e., upto 170Mpa can be achieved, and due to these factors, it is expected that BRHF membranes could give promising results when used for the purification of natural gas.
Tuning Particle Sizes and Active Sites of Ni/CeO2 Catalysts and Their Influence on Maleic Anhydride Hydrogenation
Authors: Zhang Qiuming , Liao Xin , Liu Shaobo , Wang Hao , Zhang Yin , Zhao Yongxiang
Published: June 2022

Supported metal catalysts are widely used in industrial processes, and the particle size of the active metal plays a key role in determining the catalytic activity. Herein, CeO2-supported Ni catalysts with different Ni loading and particle size were prepared by the impregnation method, and the hydrogenation performance of maleic anhydride (MA) over the Ni/CeO2 catalysts was investigated deeply. It was found that changes in Ni loading causes changes in metal particle size and active sites, which significantly affected the conversion and selectivity of MAH reaction. The conversion of MA reached the maximum at about 17.5 Ni loading compared with other contents of Ni loading because of its proper particle size and active sites. In addition, the effects of Ni grain size, surface oxygen vacancy, and Ni–CeO2 interaction on MAH were investigated in detail, and the possible mechanism for MAH over Ni/CeO2 catalysts was deduced. This work greatly deepens the fundamental understanding of Ni loading and size regimes over Ni/CeO2 catalysts for the hydrogenation of MA and provides a theoretical and experimental basis for the preparation of high-activity catalysts for MAH.
Pulsed Fluidization of Nanosilica: Rigorous Evaluation of the Efficacy of Pulsation Frequency
Authors: Asif Mohammad , Al-Ghurabi Ebrahim H. , Fatehmulla Amanullah
Published: June 2022

Assisted fluidization techniques can significantly improve the hydrodynamics of difficult- to-fluidize solids. Among these techniques, the pulsed flow strategy is highly promising owing to its cost-effectiveness and amenability to implementation for largescale processing. Using commercial-grade, highly porous nanosilica that shows strong agglomeration behavior, we implemented the pulsed flow with square-wave pulsation schemes of 0.05, 0.10, and 0.25 Hz frequencies, and compared their effectiveness in each case. Besides the conventional approach of assessing their efficacy using the pressure drop data, we have proposed a new approach in this work that consists of computing the power of the overall pressure drop transient signals. Using the theoretical value, i.e., the effective bed weight per unit area as a reference, the percentage increase in the power was 27 ± 4, 71 ± 5, and 128 ± 4, respectively, for 0.05, 0.10, and 0.25 Hz pulsation frequencies. In fact, the average pressure drop values were substantially higher when the partial bed collapse occurred between successive pulsations when compared with the case of low-frequency pulsations. The pulsation frequency also affected the evolution of local bed dynamics in various bed regions during the expansion and collapse of the bed. Moreover, the local and global pressure transients have shown interesting mutual correlations which were otherwise not evident from their individual transient profiles.
Hypolipidemic Activity of Olive Oil-Based Nanostructured Lipid Carrier Containing Atorvastatin
Authors: Elsewedy Heba S. , Shehata Tamer M. , Almostafa Mervt M. , Soliman Wafaa E.
Published: June 2022

Currently, hyperlipidemia is a growing health issue that is considered a risk factor for obesity. Controlling body weight and modifying life style in most of cases are not adequate and the condition requires medical treatment. Statin drugs (mainly Atorvastatin (ATO)), have been used broadly and for long time as medications for handling higher levels of lipid, especially bad cholesterol, which accordingly controls the prevalence of obesity. Still, the obstacle that stands in front of any formulation is the poor solubility of the drug. Low solubility of ATO came up with poor absorption as well as poor bioavailability. This paved the way for the present study, which aimed to exploit nanotechnology and develop certain nanolipid carriers that could accommodate hydrophobic drugs, such as ATO. Nanostructured lipid carrier (NLC) containing ATO was fabricated using olive oil. Olive oil is natural plant oil possessing confirmed hypolipidemic activity that would help in improving the efficacy of the formulation. Via applying the Quality by Design (QbD) approach, one NLC formula was selected to be optimized based on appropriate size and higher entrapment. Optimized ATO-NLC was scrutinized for zeta potential, in vitro study and kinetic profile. Moreover, stability testing and in vivo hypolipidemic behavior was conducted. The optimized NLC formulation seemed to show particle size (254.23 nm) with neutral zeta potential (−1.77 mV) and entrapment efficiency (69.56%). The formulation could be prolonged for 12 h and provided higher % of release (97.17%). Stability testing confirmed the role of modifying the surface of the formulation with PEG-DSPE in providing a highly stable formulation that could withstand three months storage in two altered conditions. Ultimately, optimized ATO-NLC could successfully lower total cholesterol level in rats induced with obesity and fed a high-fat diet. Remarkably, ATO-NLC prepared with olive oil, in addition to shielding its surface, would provide a stable formulation that holds up the synergistic action between olive oil and ATO.
Liposomal Form of 2,4-Dinitrophenol Lipophilic Derivatives as a Promising Therapeutic Agent for ATP Synthesis Inhibition
Authors: Vlasova Kseniya Yu. , Ostroverkhov Petr , Vedenyapina Daria , Yakimova Tamara , Trusova Alla , Lomakina Galina Yurievna , Vodopyanov Stepan Sergeevich , Grin Mikhail , Klyachko Natalia , Chekhonin Vladimir , Abakumov Maxim
Published: June 2022

Mitochondrial uncoupler 2,4-dinitrophenol (2,4-DNP) is a promising antidiabetic and antiobesity agent. Its clinical use is limited by a narrow dynamic range and accumulation in non-target sensitive organs, which results in whole-body toxicity. A liposomal formulation could enable the mentioned drawbacks to be overcome and simplify the liver-targeted delivery and sustained release of 2,4-DNP. We synthesized 2,4-DNP esters with carboxylic acids of various lipophilic degrees using carboxylic acid chloride and then loaded them into liposomes. We demonstrated the effective increase in the entrapment of 2,4-DNP into liposomes when esters were used. Here, we examined the dependence of the sustained release of 2,4-DNP from liposomes on the lipid composition and LogPoct of the ester. We posit that the optimal chain length of the ester should be close to the palmitic acid and the lipid membrane should be composed of phospholipids with a certain phase transition point depending on the desired release rate. The increased effect of the ATP synthesis inhibition of the liposomal forms of caproic and palmitic acid esters compared to free molecules in liver hepatocytes was demonstrated. The liposomes’ stability could well be responsible for this result. This work demonstrates promising possibilities for the liver-targeted delivery of the 2,4-DNP esters with carboxylic acids loaded into liposomes for ATP synthesis inhibition.