bluuebunny/crossref_metadata_2025 huggingface dataset:
Is REDD+ More of an Institutional Affair than a Market Process? The Concealed Social and Cultural Consequences of an Ongoing REDD+ Project in Kolo Hills, Tanzania
Authors: Bartholdson Örjan , Abdallah Jumanne M. , Marquardt Kristina , Salomonsson Lennart
Published: July 2019

The neo-liberal rationale behind REDD programs aims to create a market for common resources, with monetary payment incentives as the most important driver for conservation initiatives. In reality, however, the chain of implementation from UN to village, encompassing numerous processes of design, planning, and practices at distinct levels and contexts, is more institutional and political than economic. This research project follows the planning and implementation process of a REDD+ project in the Kolo Hills, Tanzania. The analysis showed that the project’s main objectives were poorly understood by the men and women of the target group, who interpreted it as yet another top-down postcolonial project. The target group’s interpretations also made them act in accordance with their own cultural rationality and logic of practice and not as the donors and project implementers had assumed. The project objectives of the payment system, consciousness awareness and engagement of the target population, thus, seem to have failed, despite the donors’ and implementers’ claim of success.
Local Adaptation and Response of Platycladus orientalis (L.) Franco Populations to Climate Change
Authors: Hu Xian-Ge , Mao Jian-Feng , El-Kassaby Yousry A. , Jia Kai-Hua , Jiao Si-Qian , Zhou Shan-Shan , Li Yue , Coops Nicholas C. , Wang Tongli
Published: July 2019

Knowledge about the local adaptation and response of forest tree populations to the climate is important for assessing the impact of climate change and developing adaptive genetic resource management strategies. However, such information is not available for most plant species. Here, based on 69 provenances tested at 19 common garden experimental sites, we developed a universal response function (URF) for tree height at seven years of age for the important and wide-spread native Chinese tree species Platycladus orientalis (L.) Franco. URF was recently used to predict the potential growth response of a population originating from any climate and growing in any climate conditions. The developed model integrated both genetic and environmental effects, and explained 55% of the total variation in tree height observed among provenances and test sites in China. We found that local provenances performed better than non-local counterparts in habitats located in central, eastern, and southwestern China, showing the evidence of local adaptation as compared to other regions. In contrast, non-local provenances outperformed local ones in peripheral areas in northern and northwestern China, suggesting an adaptational lag in these areas. Future projections suggest that the suitable habitat areas of P. orientalis would expand by 15%–39% and shift northward by 0.8–3 degrees in latitude; however, the projected tree height of this species would decline by 4%–8% if local provenances were used. If optimal provenances were used, tree height growth could be improved by 13%–15%, along with 59%–71% suitable habitat expansion. Thus, assisted migration with properly selected seed sources would be effective in avoiding maladaptation in new plantations under a changing climate for P. orientalis.
Pine Pitch Canker and Insects: Relationships and Implications for Disease Spread in Europe
Authors: Fernández-Fernández Mercedes , Naves Pedro , Witzell Johanna , Musolin Dmitry , Selikhovkin Andrey , Paraschiv Marius , Chira Danut , Martínez-Álvarez Pablo , Martín-García Jorge , Muñoz-Adalia E. , Altunisik Aliye , Cocuzza Giuseppe , Silvestro Silvia , Zamora Cristina , Diez Julio
Published: July 2019

The fungal pathogen Fusarium circinatum (Nirenberg and O’ Donnell) is the causal agent of pine pitch canker (PPC) disease, which seriously affects conifer species in forests and nurseries worldwide. In Europe, PPC is only established in the Iberian Peninsula; however, it is presumed that its range could expand through the continent in the near future. Infection caused by this fungus requires open wounds on the tree, including physical damage caused by insects. Therefore, a relationship probably occurs between PPC and a wide variety of insects. The aim of this review is to outline the taxonomic and ecological diversity of insect species with high potential association with F. circinatum in Europe and elsewhere. The insects were classified as vectors, carriers and wounding agents according to the association level with the PPC disease. In addition, we discuss the insect-mediated spreading of PPC disease in relation to the different phases of forest stand development, from seeds and seedlings in nurseries to mature stands. Lastly, to improve our predictive capacities and to design appropriate intervention measures and strategies for controlling disease dissemination by insects, variables such as geographic location, time of the year and host species should be considered. Our review provides a framework of the multiple factors that regulate the insect–host interactions and determine the success of the infection.
PARP Inhibitors in Prostate Cancer–the Preclinical Rationale and Current Clinical Development
Authors: Virtanen Verneri , Paunu Kreetta , Ahlskog Johanna K. , Varnai Reka , Sipeky Csilla , Sundvall Maria
Published: July 2019

Prostate cancer is globally the second most commonly diagnosed cancer type in men. Recent studies suggest that mutations in DNA repair genes are associated with aggressive forms of prostate cancer and castration resistance. Prostate cancer with DNA repair defects may be vulnerable to therapeutic targeting by Poly(ADP-ribose) polymerase (PARP) inhibitors. PARP enzymes modify target proteins with ADP-ribose in a process called PARylation and are in particular involved in single strand break repair. The rationale behind the clinical trials that led to the current use of PARP inhibitors to treat cancer was to target the dependence of BRCA-mutant cancer cells on the PARP-associated repair pathway due to deficiency in homologous recombination. However, recent studies have proposed therapeutic potential for PARP inhibitors in tumors with a variety of vulnerabilities generating dependence on PARP beyond the synthetic lethal targeting of BRCA1/BRCA2 mutated tumors, suggesting a wider potential than initially thought. Importantly, PARP-associated DNA repair pathways are also closely connected to androgen receptor (AR) signaling, which is a key regulator of tumor growth and a central therapeutic target in prostate cancer. In this review, we provide an extensive overview of published and ongoing trials exploring PARP inhibitors in treatment of prostate cancer and discuss the underlying biology. Several clinical trials are currently studying PARP inhibitor mono-and combination therapies in the treatment of prostate cancer. Integration of drugs targeting DNA repair pathways in prostate cancer treatment modalities allows developing of more personalized care taking also into account the genetic makeup of individual tumors.
Land Use Change Detection and Prediction in Upper Siem Reap River, Cambodia
Authors: Chim Kosal , Tunnicliffe Jon , Shamseldin Asaad , Ota Tetsuji
Published: July 2019

Siem Reap River has played a crucial role in maintaining the Angkor temple complex and livelihood of the people in the basin since the 12th century. Land use in this watershed has changed considerably over the last few decades, which is thought to have had an influence on river. This study was carried out as part of assessing the land use and climate change on hydrology of the upper Siem Reap River. The objective was to reconstruct patterns of annual deforestation from 1988 to 2018 and to explore scenarios of land use 40 and 80 years into the future. A supervised maximum likelihood classification was applied to investigate forest cover change in the last three decades. Multi-layer perceptron neural network-Markov chain (MLPNN-MC) was used to forecast land use and land cover (LULC) change for the years 2058 and 2098. The results show that there has been a significantly decreasing trend in forest cover at the rate 1.22% over the last three decades, and there would be a continuous upward trend of deforestation and downward trend of forest cover in the future. This study emphasizes the impacts of land use change on water supply for the Angkor temple complex (World Heritage Site) and the surrounding population.
CO2 and N2O Emissions from Spring Maize Soil under Alternate Irrigation between Saline Water and Groundwater in Hetao Irrigation District of Inner Mongolia, China
Authors: Wang Yu , Yang Peiling , Ren Shumei , He Xin , Wei Chenchen , Wang Shuaijie , Xu Yao , Xu Ziang , Zhang Yanxia , Ismail Hassan
Published: July 2019

Alternative irrigation between saline water and groundwater can alleviate shortages of available agricultural water while effectively slowing the adverse effects of saline water on the soil-crop system when compared with continuous irrigation with saline water and blending irrigation between saline water and groundwater. In 2018, we tested the effect on soil CO2 and N2O emissions by two types of irrigation regimes (alternating groundwater and saline water (GW-SW), and alternating groundwater, followed by two cycles of saline water (GW-SW-SW)) between groundwater and three levels of salinity of irrigation water (mineralization of 2 g/L, 3.5 g/L, and 5 g/L), analyzed the correlation between gas emissions and soil properties, calculated comprehensive global warming potential (GWP), and investigated the maize yield. The results show that, with the same alternate irrigation regime, cumulative CO2 emissions decreased with increasing irrigation water salinity, and cumulative N2O emissions increased. Cumulative CO2 emissions were higher in the GW-SW regime for the same irrigation water salinity, and cumulative N2O emissions were higher in the GW-SW-SW regime. The GW-SW-SW regime had less comprehensive GWP and maize yield as compared to the GW-SW regime. The 2 g/L salinity in both regimes showed larger comprehensive GWP and maize yield. The 3.5 g/L salinity under the GW-SW regime will be the best choice while considering that the smaller comprehensive GWP and the larger maize yield are appropriate for agricultural implication. Fertilizer type and irrigation amount can be taken into consideration in future research direction.
Negative Influences of the 4th Industrial Revolution on the Workplace: Towards a Theoretical Model of Entropic Citizen Behavior in Toxic Organizations
Authors: Coldwell David. A. L.
Published: July 2019

The 4th industrial revolution, referred to as a ‘second coming’ of the ‘digital era,’ has introduced both positive and negative effects on the workplace. While digitalization and automation have taken the drudgery out of work for some and released them to enjoy qualitative improvements at work and higher salaries, others have been thrust into low-paying work and unemployment with negative effects on their well-being and mental health. In many cases stress and threats of job loss created by digital era automation have generated negative workplace behavior and workplace outcomes. The 4th industrial revolution and its burgeoning information technology have presented widespread access to information to stakeholders and the general public about organizational business and environmental performance. This open access to information has driven toxic business leaders to maintain company profitability and environmental sustainability by pressuring employees to find solutions to difficult organizational problems with short timelines attached. Employees often are required to ‘go the extra mile’ to achieve organizational goals through forms of organizational citizenship behavior. Additionally, although organizational citizenship behavior can generate significant benefits for a company, toxic and entropic workplace outcomes can also occur from its more extreme manifestations arising from the stressful circumstances digitalization and automation of work have created. The methodological approach adopted in this paper is a secondary data analysis which uses reliable and valid sources of report documentation to corroborate a theoretical model of organizational citizenship behavior entropy. The theoretical model suggests that extreme forms of organizational citizenship behavior associated with the digital era can create toxic leaders and business organizations that lead to organizational entropy.
Efficacy of Polyunsaturated Fatty Acids on Inflammatory Markers in Patients Undergoing Dialysis: A Systematic Review with Network Meta-Analysis of Randomized Clinical Trials
Authors: Wu Po-Kuan , Yeh Shu-Ching , Li Shan-Jen , Kang Yi-No
Published: July 2019

The effects of polyunsaturated fatty acids (PUFAs) on inflammatory markers among patients receiving dialysis have been discussed for a long time, but previous syntheses made controversial conclusion because of highly conceptual heterogeneity in their synthesis. Thus, to further understanding of this topic, we comprehensively gathered relevant randomized clinical trials (RCTs) before April 2019, and two authors independently extracted data of C-reactive protein (CRP), high-sensitivity C-reactive protein (hs-CRP), and interleukin-6 (IL-6) for conducting network meta-analysis. Eighteen eligible RCTs with 962 patients undergoing dialysis were included in our study. The result showed that with placebo as the reference, PUFAs was the only treatment showing significantly lower CRP (weighted mean difference (WMD): −0.37, 95% confidence interval (CI): −0.07 to −0.68), but the CRP in PUFAs group was not significantly lower than vitamin E, PUFAs plus vitamin E, or medium-chain triglyceride. Although no significant changes were noted for hs-CRP and IL-6 levels, PUFAs showed the best ranking among treatments according to surface under the cumulative ranking. Therefore, PUFAs could be a protective option for patients receiving dialysis in clinical practice.
MAS NMR on a Red/Far-Red Photochromic Cyanobacteriochrome All2699 from Nostoc
Authors: Xu Qian-Zhao , Bielytskyi Pavlo , Otis James , Lang Christina , Hughes Jon , Zhao Kai-Hong , Losi Aba , Gärtner Wolfgang , Song Chen
Published: July 2019

Unlike canonical phytochromes, the GAF domain of cyanobacteriochromes (CBCRs) can bind bilins autonomously and is sufficient for functional photocycles. Despite the astonishing spectral diversity of CBCRs, the GAF1 domain of the three-GAF-domain photoreceptor all2699 from the cyanobacterium Nostoc 7120 is the only CBCR-GAF known that converts from a red-absorbing (Pr) dark state to a far-red-absorbing (Pfr) photoproduct, analogous to the more conservative phytochromes. Here we report a solid-state NMR spectroscopic study of all2699g1 in its Pr state. Conclusive NMR evidence unveils a particular stereochemical heterogeneity at the tetrahedral C31 atom, whereas the crystal structure shows exclusively the R-stereochemistry at this chiral center. Additional NMR experiments were performed on a construct comprising the GAF1 and GAF2 domains of all2699, showing a greater precision in the chromophore–protein interactions in the GAF1-2 construct. A 3D Pr structural model of the all2699g1-2 construct predicts a tongue-like region extending from the GAF2 domain (akin to canonical phytochromes) in the direction of the chromophore, shielding it from the solvent. In addition, this stabilizing element allows exclusively the R-stereochemistry for the chromophore-protein linkage. Site-directed mutagenesis performed on three conserved motifs in the hairpin-like tip confirms the interaction of the tongue region with the GAF1-bound chromophore.
Adipogenesis: A Necessary but Harmful Strategy
Authors: Hafidi Mohammed El , Buelna-Chontal Mabel , Sánchez-Muñoz Fausto , Carbó Roxana
Published: July 2019

Obesity is considered to significantly increase the risk of the development of a vast range of metabolic diseases. However, adipogenesis is a complex physiological process, necessary to sequester lipids effectively to avoid lipotoxicity in other tissues, like the liver, heart, muscle, essential for maintaining metabolic homeostasis and has a crucial role as a component of the innate immune system, far beyond than only being an inert mass of energy storage. In pathophysiological conditions, adipogenesis promotes a pro-inflammatory state, angiogenesis and the release of adipokines, which become dangerous to health. It results in a hypoxic state, causing oxidative stress and the synthesis and release of harmful free fatty acids. In this review, we try to explain the mechanisms occurring at the breaking point, at which adipogenesis leads to an uncontrolled lipotoxicity. This review highlights the types of adipose tissue and their functions, their way of storing lipids until a critical point, which is associated with hypoxia, inflammation, insulin resistance as well as lipodystrophy and adipogenesis modulation by Krüppel-like factors and miRNAs.
Cell-Free Nucleic Acids and their Emerging Role in the Pathogenesis and Clinical Management of Inflammatory Bowel Disease
Authors: Kubiritova Zuzana , Radvanszky Jan , Gardlik Roman
Published: July 2019

Cell-free nucleic acids (cfNAs) are defined as any nucleic acids that are present outside the cell. They represent valuable biomarkers in various diagnostic protocols such as prenatal diagnostics, the detection of cancer, and cardiovascular or autoimmune diseases. However, in the current literature, little is known about their implication in inflammatory bowel disease (IBD). IBD is a group of multifactorial, autoimmune, and debilitating diseases with increasing incidence worldwide. Despite extensive research, their etiology and exact pathogenesis is still unclear. Since cfNAs were observed in other autoimmune diseases and appear to be relevant in inflammatory processes, their role in the pathogenesis of IBD has also been suggested. This review provides a summary of knowledge from the available literature about cfDNA and cfRNA and the structures involving them such as exosomes and neutrophil extracellular traps and their association with IBD. Current studies showed the promise of cfNAs in the management of IBD not only as biomarkers distinguishing patients from healthy people and differentiating active from inactive disease state, but also as a potential therapeutic target. However, the detailed biological characteristics of cfNAs need to be fully elucidated in future experimental and clinical studies.
Extracellular Vesicles Released by Colorectal Cancer Cell Lines Modulate Innate Immune Response in Zebrafish Model: The Possible Role of Human Endogenous Retroviruses
Authors: Ferrari Luca , Cafora Marco , Rota Federica , Hoxha Mirjam , Iodice Simona , Tarantini Letizia , Dolci Maria , Delbue Serena , Pistocchi Anna , Bollati Valentina
Published: July 2019

Extracellular vesicles (EVs) are important components of the metastatic niche and are crucial in infiltration, metastasis, and immune tolerance processes during tumorigenesis. We hypothesized that human endogenous retroviruses (HERV) positive EVs derived from tumor cellsmay have a role in modulating the innate immune response. The study was conducted in two different colorectal cancer cell lines, representing different stages of cancer development: Caco-2, derived from a non-metastatic colorectal adenocarcinoma, and SK-CO-1, derived from metastatic colorectal adenocarcinoma (ascites). Both cell lines were treated with decitabine to induce global hypomethylation and to reactivate HERV expression. EVs were quantified by nanoparticle tracking analysis, and HERV-positive EV concentrations were measured by flow cytometry. The effect of EVs isolated from both untreated and decitabine-treated cells on the innate immune response was evaluated by injecting them in zebrafish embryos and then assessing Interleukin 1β (IL1-β), Interleukin 10 (IL-10), and the myeloperoxidase (mpx) expression levels by real-time qPCR. Interestingly, HERV-K positive EVs concentrations were significantly associated with a reduced expression of IL1-β and mpx, supporting our hypothesis that HERV-positive EVs may act as immunomodulators in tumor progression. The obtained results open new perspectives about the modulation of the immune response in cancer therapy.
Performance Evaluation and Dimensional Analysis of Multistage Helicoaxial Pump for Two-Phase Flow
Authors: Patil Abhay , Gudigopuram Sujan , Ayyildiz Burak , Delgado Adolfo , Morrison Gerald
Published: July 2019

A four stage helicoaxial pump was tested under varying operating conditions. A range of inlet pressures, rotational speeds (3000, 3600 rpm), and gas void fractures (GVFs) were considered for two fluid viscosities. The head developed and power input to run the pump were recorded. Head, power input and efficiency decrease as the GVF increases with best efficiency point (BEP) moving towards lower flow rate conditions. Dimensional analysis was conducted to evaluate the applicability of current affinity laws to the two-phase flow performance of the pump under consideration. Dimensionless head coefficient and power coefficients were defined for two-phase flow, considering the homogeneity in the two-phase fluid properties. Deviations in the two-phase affinity coefficients from the common law curve increases with GVF. To bridge this gap, a new correlation is proposed with a revised flow coefficient that allows all the head coefficient data to collapse on a single line with a greater degree of accuracy.
New Logistics Paradigms in Poland
Authors: Gołembska Elżbieta
Published: July 2019

This article presents the results of theoretical and empirical studies on the search for new logistics development paradigms needed to meet the challenges of the twenty-first century global economy. The author reviews the major factors determining the effectiveness of international processes and operations, including logistics success potentials in supply chains. Furthermore, the article proposes methods for measuring logistics performance, specifically the logit model and the model of fuzzy logic, which could be used to assess whether a firm’s planned investments in logistics infrastructure will end in success or failure. The article also evaluates the impact of logistics on the development of international trade in the practice of Polish firms. The studies cover a period before Poland’s accession to the European Union (EU) (2000 to 2004), and after the accession, from 2005 to 2016, and are supplemented with a forecast for logistics development in Polish firms until 2020.
The Mechanical Properties and Chloride Resistance of Concrete Reinforced with Hybrid Polypropylene and Basalt Fibres
Authors: Hu Xinyu , Guo Yihong , Lv Jianfu , Mao Jize
Published: July 2019

This paper aims to investigate the effect of the polypropylene fibre (PP) and basalt fibre (BF), singly or in hybridization, on the workability, mechanical, chloride resistance and pore structure characteristics of concrete. Sixteen mixtures consisting of PP and BF, both at volume content of 0.0, 0.1, 0.2 and 0.3%, were fabricated, and the slump, compressive, splitting tensile, flexural and charge passed were tested. The results show the hybridization of the PP and BF can improve three types of strength of concrete in comparison to their single fibre. Nevertheless, the hybridization is not always conducive, and the synergy of fibres is proposed and divided into positive and negative effects. The combination of the PP and BF both at content of 0.1% achieves the best mechanical performance, and is recommended for practical usage. Incorporating fibres reduces the chloride resistance of concrete, and the hybridization is helpless to this phenomenon; even the reduction is intensified at a highly hybrid fibre volume. However, increasing the curing age can mitigate this adverse effect caused by fibres. Furthermore, the microstructures were explored to elucidate the macro-properties of concrete in terms of interface and pore structure.
Design and Development of Hybrid Al2O3 Based Composites with Toughening and Self-Lubricating Second-Phase Inclusions
Authors: Akhtar Syed Sohail , Waqar Taha , Hakeem Abbas Saeed , Arif Abul Fazal M. , Al-Athel Khaled Saleh
Published: July 2019

Polycrystalline ceramics, such as alumina (Al2O3), are brittle and they generally wear by fracture mechanism, which limits their potential in tribological applications. In the present work, computational design tools are used to develop hybrid Al2O3 composites reinforced with best combinations of toughening and self-lubricating second-phase particles for cutting tool inserts in dry machining applications. A mean-field homogenization approach and J-integral based fracture toughness models are employed to predict the effective structural properties (such as elastic modulus and fracture toughness) and related to the intrinsic attributes of second- phase inclusions in Al2O3 matrix. Silicon carbide (SiC), boron nitride (cBN and hBN), zirconia (ZrO2), graphite, titanium dioxide (TiO2), and titanium carbide (TiC) were found the most suitable candidates to be added in Al2O3 matrix as individual or hybrid combinations. A series of samples including standalone Al2O3, single inclusion composites (Al2O3/SiC, Al2O3/cBN) and hybrid composites (Al2O3/SiC/cBN, Al2O3/SiC/TiO2 and Al2O3/SiC/graphite) are sintered by Spark Plasma Sintering (SPS) for validation purpose. Properties of the sintered composites are measured and compared with the proposed computational material design. Composition and phase transformation of the sintered samples are studied using X-ray diffraction (XRD) and Raman spectroscopy, while their morphology is studied using Field Emission Scanning Electron Microscope (FESEM). The presented nontraditional material design approach is found to significantly reduce experimental time and cost of materials in developing toughened and anti-friction ceramic composites.
Improving Mechanical Properties and Reaction to Fire of EVA/LLDPE Blends for Cable Applications with Melamine Triazine and Bentonite Clay
Authors: Sanchez-Olivares Guadalupe , Sanchez-Solis Antonio , Manero Octavio , Pérez-Chávez Ricardo , Jaramillo Mario , Alongi Jenny , Carosio Federico
Published: July 2019

The high flame-retardant loading required for ethylene-vinyl acetate copolymer blends with polyethylene (EVA-PE) employed for insulation and sheathing of electric cables represents a significant limitation in processability and final mechanical properties. In this work, melamine triazine (TRZ) and modified bentonite clay have been investigated in combination with aluminum trihydroxide (ATH) for the production of EVA-PE composites with excellent fire safety and improved mechanical properties. Optimized formulations with only 120 parts per hundred resin (phr) of ATH can achieve self-extinguishing behavior according to the UL94 classification (V0 rating), as well as reduced combustion kinetics and smoke production. Mechanical property evaluation shows reduced stiffness and improved elongation at break with respect to commonly employed EVA-PE/ATH composites. The reduction in filler content also provides improved processability and cost reductions. The results presented here allow for a viable and halogen-free strategy for the preparation of high performing EVA-PE composites.
A Novel Polyhalogenated Monoterpene Induces Cell Cycle Arrest and Apoptosis in Breast Cancer Cells
Authors: El Gaafary Menna , Hafner Susanne , Lang Sophia J. , Jin Lu , Sabry Omar M. , Vogel Carl V. , Vanderwal Christopher D. , Syrovets Tatiana , Simmet Thomas
Published: July 2019

Breast cancer is the most common cancer type and a primary cause of cancer mortality among females worldwide. Here, we analyzed the anticancer efficacy of a novel bromochlorinated monoterpene, PPM1, a synthetic analogue of polyhalogenated monoterpenes from Plocamium red algae and structurally similar non-brominated monoterpenes. PPM1, but not the non-brominated monoterpenes, decreased selectively the viability of several triple-negative as well as triple-positive breast cancer cells with different p53 status without significantly affecting normal breast epithelial cells. PPM1 induced accumulation of triple-negative MDA-MB-231 cells with 4N DNA content characterized by decreased histone H3-S10/T3 phosphorylation indicating cell cycle arrest in the G2 phase. Western immunoblot analysis revealed that PPM1 treatment triggered an initial rapid activation of Aurora kinases A/B/C and p21Waf1/Cip1 accumulation, which was followed by accumulation of polyploid >4N cells. Flow cytometric analysis showed mitochondrial potential disruption, caspase 3/7 activation, phosphatidylserine externalization, reduction of the amount polyploid cells, and DNA fragmentation consistent with induction of apoptosis. Cell viability was partially restored by the pan-caspase inhibitor Z-VAD-FMK indicating caspase contribution. In vivo, PPM1 inhibited growth, proliferation, and induced apoptosis in MDA-MB-231 xenografted onto the chick chorioallantoic membrane. Hence, Plocamium polyhalogenated monoterpenes and synthetic analogues deserve further exploration as promising anticancer lead compounds.
Zinc-Chelating Mechanism of Sea Cucumber (Stichopus japonicus)-Derived Synthetic Peptides
Authors: Liu Xiaoyang , Wang Zixu , Yin Fawen , Liu Yuxin , Qin Ningbo , Nakamura Yoshimasa , Shahidi Fereidoon , Yu Chenxu , Zhou Dayong , Zhu Beiwei
Published: July 2019

In this study, three synthetic zinc-chelating peptides (ZCPs) derived from sea cucumber hydrolysates with limited or none of the common metal-chelating amino-acid residues were analyzed by flame atomic absorption spectroscopy, circular dichroism spectroscopy, size exclusion chromatography, zeta-potential, Fourier transform infrared spectroscopy, Raman spectroscopy and nuclear magnetic resonance spectroscopy. The amount of zinc bound to the ZCPs reached maximum values with ZCP:zinc at 1:1, and it was not further increased by additional zinc presence. The secondary structures of ZCPs were slightly altered, whereas no formation of multimers was observed. Furthermore, zinc increased the zeta-potential value by neutralizing the negatively charged residues. Only free carboxyl in C-terminus of ZCPs was identified as the primary binding site of zinc. These results provide the theoretical foundation to understand the mechanism of zinc chelation by peptides.
Optimizing Ultrasound-Assisted Deep Eutectic Solvent Extraction of Bioactive Compounds from Chinese Wild Rice
Authors: Zeng Jia , Dou Yuqing , Yan Ning , Li Na , Zhang Huaibao , Tan Jia-Neng
Published: July 2019

In this study, deep eutectic solvents (DESs) were used for the ultrasound-assisted extraction (UAE) of valuable bioactive compounds from Chinese wild rice (Zizania spp.). To this end, 7 different choline chloride (CC)-based DESs were tested as green extraction solvents. Choline chloride/1,4-butanediol (DES-2) exhibited the best extraction efficiency in terms of parameters such as the total flavonoid content (TFC), total phenolic content (TPC), and free radical scavenging capacity (DPPH● and ABTS●+). Subsequently, the UAE procedure using 76.6% DES-2 was also optimized: An extraction temperature of 51.2 °C and a solid–liquid ratio of 37.0 mg/mL were considered optimal by a Box–Behnken experiment. The optimized extraction procedure proved efficient for the extraction of 9 phenolic and 3 flavonoid compounds from Chinese wild rice as determined by quantification based on ultra-performance liquid chromatography–triple quadrupole tandem mass spectrometry (UPLC-QqQ-MS). This work, thus, demonstrates the possibility of customizing green solvents that offer greater extraction capacity than that of organic solvents.
Daily Life Restrictions are Common and Associated with Health Concerns and Dietary Challenges in Adult Celiac Disease Patients Diagnosed in Childhood
Authors: Leinonen Heini , Kivelä Laura , Lähdeaho Marja-Leena , Huhtala Heini , Kaukinen Katri , Kurppa Kalle
Published: July 2019

The prevalence and associated factors of daily life restrictions due to a gluten-free diet in adult celiac disease patients diagnosed in childhood are poorly known. We investigated these issues by collecting the medical data of 955 pediatric patients and sending questionnaires evaluating various health outcomes to the 559 patients who had reached adulthood. Of the 231 respondents, 46% reported everyday life restrictions caused by dietary treatment. Compared with those without restrictions, they more often had anemia at diagnosis (37% vs. 22%, p = 0.014), but the groups were comparable in other diagnostic features. In adulthood, patients with restrictions reported more overall symptoms (32% vs. 17%, p = 0.006), although the symptoms measured with the Gastrointestinal Symptom Rating Scale questionnaire were comparable. Despite strict dietary adherence in both groups, the experience of restrictions was associated with dietary challenges (34% vs. 9%, p < 0.001), health concerns (22% vs. 13%, p = 0.050), and lower vitality scores in the Psychological General Well-Being questionnaire. The groups did not differ in their current age, socioeconomic status, family history of celiac disease, general health or health-related lifestyle, the presence of co-morbidities, or regular follow up. Our results encourage healthcare professionals to discuss the possible health concerns and dietary challenges with patients to avoid unnecessary daily life restrictions, especially when young patients start to take responsibility for their treatment.
Raw Cow’s Milk Reduces Allergic Symptoms in a Murine Model for Food Allergy—A Potential Role for Epigenetic Modifications
Authors: Abbring Suzanne , Wolf Johanna , Ayechu-Muruzabal Veronica , Diks Mara A.P. , Alashkar Alhamwe Bilal , Alhamdan Fahd , Harb Hani , Renz Harald , Garn Holger , Garssen Johan , Potaczek Daniel P. , van Esch Betty C.A.M.
Published: July 2019

Epidemiological studies identified raw cow’s milk consumption as an important environmental exposure that prevents allergic diseases. In the present study, we investigated whether raw cow’s milk has the capacity to induce tolerance to an unrelated, non-milk, food allergen. Histone acetylation of T cell genes was investigated to assess potential epigenetic regulation. Female C3H/HeOuJ mice were sensitized and challenged to ovalbumin. Prior to sensitization, the mice were treated with raw milk, processed milk, or phosphate-buffered saline for eight days. Allergic symptoms were assessed after challenge and histone modifications in T cell-related genes of splenocyte-derived CD4+ T cells and the mesenteric lymph nodes were analyzed after milk exposure and after challenge. Unlike processed milk, raw milk decreased allergic symptoms. After raw milk exposure, histone acetylation of Th1-, Th2-, and regulatory T cell-related genes of splenocyte-derived CD4+ T cells was higher than after processed milk exposure. After allergy induction, this general immune stimulation was resolved and histone acetylation of Th2 genes was lower when compared to processed milk. Raw milk reduces allergic symptoms to an unrelated, non-milk, food allergen in a murine model for food allergy. The activation of T cell-related genes could be responsible for the observed tolerance induction, which suggested that epigenetic modifications contribute to the allergy-protective effect of raw milk.
Exploring the Science behind Bifidobacterium breve M-16V in Infant Health
Authors: Wong Chyn Boon , Iwabuchi Noriyuki , Xiao Jin-zhong
Published: July 2019

Probiotics intervention has been proposed as a feasible preventative approach against adverse health-related complications in infants. Nevertheless, the umbrella concept of probiotics has led to a massive application of probiotics in a range of products for promoting infant health, for which the strain-specificity, safety and efficacy findings associated with a specific probiotics strain are not clearly defined. Bifidobacterium breve M-16V is a commonly used probiotic strain in infants. M-16V has been demonstrated to offer potential in protecting infants from developing the devastating necrotising enterocolitis (NEC) and allergic diseases. This review comprehends the potential beneficial effects of M-16V on infant health particularly in the prevention and treatment of premature birth complications and immune-mediated disorders in infants. Mechanistic studies supporting the use of M-16V implicated that M-16V is capable of promoting early gut microbial colonisation and may be involved in the regulation of immune balance and inflammatory response to protect high-risk infants from NEC and allergies. Summarised information on M-16V has provided conceptual proof of the use of M-16V as a potential probiotics candidate aimed at promoting infant health, particularly in the vulnerable preterm population.
A Brief Review of Genetic Approaches to the Study of Food Preferences: Current Knowledge and Future Directions
Authors: Robino Antonietta , Concas Maria Pina , Catamo Eulalia , Gasparini Paolo
Published: July 2019

Genetic variation plays a crucial role in individual differences in food preferences which ultimately influence food selection and health. Our current understanding of this pathway has been informed through twin studies (to assess the heritability of food preferences), candidate gene studies, and genome-wide association studies (GWAS). However, most of this literature is mainly focused on genes previously identified as having taste or smell functions. New data suggests that genes not associated with taste or smell perception may be involved in food preferences and contribute to health outcomes. This review highlights these emerging findings and suggests a polygenic risk assessment approach to explore new relationships between food preferences and health risks.
Thermal Stability, Fire and Smoke Behaviour of Epoxy Composites Modified with Plant Waste Fillers
Authors: Salasinska Kamila , Barczewski Mateusz , Borucka Monika , Górny Rafał L. , Kozikowski Paweł , Celiński Maciej , Gajek Agnieszka
Published: July 2019

The influence of plant fillers on the flammability and smoke emission of natural composites was investigated. Epoxy composites with 15, 25, and 35 wt % of walnut and hazelnut shell, as well as sunflower husk, were prepared and examined. The ground organic components were characterized by grain size distribution, thermogravimetric analysis (TGA) and microstructure observations (SEM). The composite materials were subjected to dynamic mechanical analysis (DMA) and structural evaluation with scanning electron microscopy. Cone calorimeter tests and TGA determined the influence of plant waste filler addition on thermal stability and flammability. Moreover, the semi-volatile and volatile compounds that evolved during the thermal decomposition of selected samples were identified using a steady state tube furnace and a gas chromatograph with a mass spectrometer. The intensity of the degradation reduced as a function of increasing filler content, while the yield of residue corresponded to the amount of lignin that is contained in the tested plants. Moreover, the incorporation of agricultural waste materials resulted in the formation of a char layer, which inhibits the burning process. The yield of char depended on the amount and type of the filler. The composites containing ground hazelnut shell formed swollen char that was shaped in multicellular layers, similar to intumescent fire retardants.
Effects of Chain Length of Chitosan Oligosaccharides on Solution Properties and Complexation with siRNA
Authors: Delas Tim , Mock-Joubert Maxime , Faivre Jimmy , Hofmaier Mirjam , Sandre Olivier , Dole François , Chapel Jean Paul , Crépet Agnès , Trombotto Stéphane , Delair Thierry , Schatz Christophe
Published: July 2019

In the context of gene delivery, chitosan has been widely used as a safe and effective polycation to complex DNA, RNA and more recently, siRNA. However, much less attention has been paid to chitosan oligosaccharides (COS) despite their biological properties. This study proposed to carry out a physicochemical study of COS varying in degree of polymerization (DP) from 5 to 50, both from the point of view of the solution properties and the complexing behavior with siRNA. The main parameters studied as a function of DP were the apparent pKa, the solubility versus pH, the binding affinity with siRNA and the colloidal properties of complexes. Some parameters, like the pKa or the binding enthalpy with siRNA, showed a marked transition from DP 5 to DP 13, suggesting that electrostatic properties of COS vary considerably in this range of DP. The colloidal properties of siRNA/COS complexes were affected in a different way by the COS chain length. In particular, COS of relatively high DP (≥50) were required to form small complex particles with good stability.
Use of a LHFB Device for Testing Mode III in a Composite Laminate
Authors: Bertorello Carlos , Argüelles Antonio , Mollón Victoria , Bonhomme Jorge , Viña Isabel , Viña Jaime
Published: July 2019

The present paper studies the fatigue delamination behaviour of an epoxy/carbon composite material under mode III loading using a longitudinal half fixed beam (LHFB) device initially designed for mode III static tests of composite materials formed by the stacking of plies. For this purpose, a series of tests was carried out at different levels of loading representative of the fatigue behaviour of the material, from the crack onset phase through the delamination phase to final fracture. The experimental results were treated statistically, obtaining the values of the fatigue limit for probabilities of fracture of 5% and 50%. Finally, a fractographic analysis of the fracture surfaces was performed which allowed us to identify the same characteristic patterns of static mode III fracture, namely broken fibres, cusps and saw-teeth, in addition to a new morphology consisting of the formation of agglomerations of resin produced by the friction between the lips of the specimen in the fracture zone that point to dynamic mode III fracture. These agglomerations eventually crack and become detached from the fibres, leaving these free of resin.
Flexural Properties and Mean Intrinsic Flexural Strength of Old Newspaper Reinforced Polypropylene Composites
Authors: Tarrés Quim , Soler Jordi , Rojas-Sola José Ignacio , Oliver-Ortega Helena , Julián Fernando , Espinach F. Xavier , Mutjé Pere , Delgado-Aguilar Marc
Published: July 2019

Newspapers have a limited lifespan, and therefore represent a focus of used and disposed paper. While these refuses have a considerable value and can be easily recycled, a considerable fraction ends in landfill. The authors show the possibility of adding value to used newspaper and enlarge its value chain. Old newspaper incorporates a high amount of wood fibers able to be used as reinforcement. On the other hand, this material also incorporates inks and other components inherent to newspaper production. In this work, the authors disintegrate old newspaper to recover and individualize wood fibers. A morphological analysis showed that the recovered fibers had aspect ratios higher than 10, revealing, a priori, their strengthening capabilities. Thereupon, these fibers were compounded with polypropylene at different contents, ranging from 20% to 50% w/w. It is well known that wood fibers are hydrophilic, while polyolefin are hydrophobic. This is a drawback to obtaining strong interfaces. Thus, two sets of composites were produced, with and without a coupling agent. The results showed that uncoupled composites increased the flexural strength of the matrix but reached an equilibrium point from which adding more reinforcement did not changer the flexural strength. On the other hand, the coupled composites showed a linear increase of the flexural strength against the reinforcement content. The flexural moduli of the coupled and uncoupled composites were very similar and evolved linearly with the reinforcement content.
First Results of Phytoplankton Spatial Dynamics in Two NW-Mediterranean Bays from Chlorophyll-a Estimates Using Sentinel 2: Potential Implications for Aquaculture
Authors: Soriano-González Jesús , Angelats Eduard , Fernández-Tejedor Margarita , Diogene Jorge , Alcaraz Carles
Published: July 2019

Shellfish aquaculture has a major socioeconomic impact on coastal areas, thus it is necessary to develop support tools for its management. In this sense, phytoplankton monitoring is crucial, as it is the main source of food for shellfish farming. The aim of this study was to assess the applicability of Sentinel 2 multispectral imagery (MSI) to monitor the phytoplankton biomass at Ebro Delta bays and to assess its potential as a tool for shellfish management. In situ chlorophyll-a data from Ebro Delta bays (NE Spain) were coupled with several band combination and band ratio spectral indices derived from Sentinel 2A levels 1C and 2A for time-series mapping. The best results (AIC = 72.17, APD < 10%, and MAE < 0.7 mg/m3) were obtained with a simple blue-to-green ratio applied over Rayleigh corrected images. Sentinel 2–derived maps provided coverage of the farm sites at both bays allowing relating the spatiotemporal distribution of phytoplankton with the environmental forcing under different states of the bays. The applied methodology will be further improved but the results show the potential of using Sentinel 2 MSI imagery as a tool for assessing phytoplankton spatiotemporal dynamics and to encourage better future practices in the management of the aquaculture in Ebro Delta bays.
A BDS-2/BDS-3 Integrated Method for Ultra-Rapid Orbit Determination with the Aid of Precise Satellite Clock Offsets
Authors: Wang Qianxin , Hu Chao , Zhang Kefei
Published: July 2019

The accuracy of ultra-rapid orbits is a key parameter for the performance of GNSS (Global Navigation Satellite System) real-time or near real-time precise positioning applications. The quality of the current BeiDou demonstration system (BDS) ultra-rapid orbits is lower than that of GPS, especially for the new generational BDS-3 satellites due to the fact that the availability of the number of ground tracking stations is limited, the geographic distribution of these stations is poor, and the data processing strategies adopted are not optimal. In this study, improved data processing strategies for the generation of ultra-rapid orbits of BDS-2/BDS-3 satellites are investigated. This includes both observed and predicted parts of the orbit. First, the predicted clock offsets are taken as constraints in the estimation process to reduce the number of the unknown parameters and improve the accuracy of the parameter estimates of the orbit. To obtain more accurate predicted clock offsets for the BDS’ orbit determination, a denoising method (also called the Tikhonov regularization algorithm), inter-satellite correlation, and the partial least squares method are all incorporated into the clock offsets prediction model. Then, the Akaike information criterion (AIC) is used to determine the arc length in the estimation models by taking the optimal arc length in the estimation of the initial orbit states into consideration. Finally, a number of experiments were conducted to evaluate the performance of the ultra-rapid orbits resulting from the proposed methods. Results showed that: (1) Compared with traditional models, the accuracy improvement of the predicted clock offsets from the proposed methods were 40.5% and 26.1% for BDS-2 and BDS-3, respectively; (2) the observed part of the orbits can be improved 9.2% and 5.0% for BDS-2 and BDS-3, respectively, by using the predicted clock offsets as constraints; (3) the accuracy of the predicted part of the orbits showed a high correlation with the AIC value, and the accuracy of the predicted orbits could be improved up to 82.2%. These results suggest that the approaches proposed in this study can significantly enhance the accuracy of the ultra-rapid orbits of BDS-2/BDS-3 satellites.