bluuebunny/crossref_metadata_2025 huggingface dataset:
Features of Grammatical Writing Competence among Early Writers in a Norwegian School Context
Authors: Nygård Mari , Hundal Anne Kathrine
Published: January 2024

In this article, we investigate how grammatical competence is manifested in young students’ written texts and how this contributes to the students’ overall writing competence. We pose the following two research questions: (i) Which grammatical features appear in a selection of young students’ texts? and (ii) What do these features reveal about different aspects of the students’ grammatical writing competence? The empirical fundament for this study is a representative sample of texts gathered through the project FUS—Functional Writing in the First School Years. Our primary material amounts to a total of 534 texts written by first- and second-grade students (ages 6–7). The students have completed two writing tasks, in which they were asked to write one descriptive and one narrative text. In our analyses, we see grammatical writing competence as consisting of several sub-competencies—namely, grammatical repertoire, grammatical complexity, grammatical variation, and grammatical choice. Our analyses show that the grammatical repertoire of beginner students is well-developed. The frequency of specific grammatical features differs between the two writing tasks, underpinning the argument that certain text types trigger certain grammatical choices.
The Ecophysiological Response of Olive Trees under Different Fruit Loads
Authors: Kokkotos Efthymios , Zotos Anastasios , Patakas Angelos
Published: January 2024

Olive trees have a unique reproductive pattern marked by biennial fruiting. This study examined the repercussions of alternate fruit bearing on the water relations of olive trees and the associated ecophysiological mechanisms. The experiment spanned two consecutive years: the “ON” year, characterized by a high crop load, and the “OFF” year, marked by minimal fruit production. Key ecophysiological parameters, including sap flow, stomatal conductance, and photosynthetic rate, were monitored in both years. Pre-dawn water potential was measured using continuous stem psychrometers and the pressure chamber technique. Biochemical analyses focused on non-structural carbohydrate concentrations (starch, sucrose, and mannitol) and olive leaves’ carbon-stable isotope ratio (δ13C). Results revealed a higher leaf gas exchange rate during the “ON” year, leading to an average 29.3% increase in water consumption and a 40.78% rise in the photosynthetic rate. Higher water usage during the “ON” year resulted in significantly lower (43.22% on average) leaf water potential. Sucrose and starch concentrations were also increased in the “ON” year, while there were no significant differences in mannitol concentration. Regarding the carbon-stable isotope ratio, leaves from the “OFF” year exhibited significantly higher δ13C values, suggesting a higher resistance to the CO2 pathway from the atmosphere to carboxylation sites compared to the “ON” year plants.
Severe Early-Onset Intrahepatic Cholestasis of Pregnancy Following Ovarian Hyperstimulation Syndrome with Pulmonary Presentation after In Vitro Fertilization: Case Report and Systematic Review of Case Reports
Authors: Dumančić Stipe , Mikuš Mislav , Palčić Zdenka , Habek Dubravko , Tešanović Mara , Mimica Marko Dražen , Marušić Jelena
Published: January 2024

Background: Intrahepatic cholestasis of pregnancy (ICP) is the most common pregnancy-related liver disease, usually presented in the third trimester with pruritus, elevated transaminase, and serum total bile acids. Evidence shows that it can be developed in the first trimester, more commonly after in vitro fertilization (IVF) procedures, with the presence of ovarian hyperstimulation syndrome (OHSS). Methods: A literature search was conducted in the PubMed/MEDLINE database of case reports/studies reporting early-onset ICP in spontaneous and IVF pregnancies published until July 2023. Results: Thirty articles on early-onset ICP were included in the review analysis, with 19 patients who developed ICP in spontaneous pregnancy and 15 patients who developed ICP in IVF pregnancies with or without OHSS. Cases of 1st and 2nd trimester ICP in terms of “early-onset” ICP were pooled to gather additional findings. Conclusions: Proper monitoring should be applied even before expected pregnancy and during IVF procedures in patients with known risk factors for OHSS and ICP development (patient and family history), with proper progesterone supplementation dosage and genetic testing in case of ICP recurrence.
Consumers’ Marketing Channel Choice and the Impact on Logistics and Operations: A Systematic Literature Review of the Fresh Food and Grocery Sector
Authors: Alkhiyami Hassan , Kerbache Laoucine , Hadid Majed
Published: January 2024

Background: Diverse marketing channels have been developed with the advent of digitalization. In the fresh food and grocery retail sector, consumers may have a large choice of channels to shop from. In this case, an analysis of their behavior is crucial for retailers. Therefore, the present paper provides a state-of-the-art review of existing papers dealing with consumers’ channel choice when buying groceries. Methods: A systematic literature review (SLR) is performed following the Preferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA) procedure, identifying 36 research papers published in the last decade. Results: The results present the principal methodologies adopted by the analyzed research papers to address this topic, along with the various channels available to consumers to date. Other important outcomes of this review include the main variables that can affect consumer choice when deciding between the available channels and the limitations of the analyzed papers, along with suggestions for future research directions to address these limitations. Conclusions: This paper discusses the essential effects of consumers’ channel choice on the logistics and operation services of grocery retailers. It also provides an integrative framework linking the influencing factors of consumer choice with outcomes directly impacting grocery retailers’ logistics services.
Nonlinear Dynamic Analysis of a Spur Gear Pair System with Wear Considering the Meshing Position
Authors: Geng Zhibo , Chen Min , Wang Jiao , Xia Yu , Kong Yun , Xiao Ke
Published: January 2024

In this paper, a nonlinear dynamic model of a parallel shaft gear system consisting of two involute spur gears is developed to investigate the coupling effect between the gradual surface wear of gear teeth over time and nonlinear dynamic characteristics. A uniform wear model that accounts for how the volumetric wear of the gear teeth affect their meshing position, backlash, and stiffness is proposed. Additionally, a nonlinear dynamic model with six degrees of freedom is described that considers friction, time-varying gear backlash, and time-varying meshing stiffness. The proposed model significantly changes the mesh stiffness, not only in terms of value but also in terms of contact ratio. Furthermore, the nonlinear dynamic characteristics of the gear system vary significantly. It is found that the gradual wear of gear teeth affects the meshing position and further has a significant impact on the nonlinear dynamic characteristics of the spur gear system. This paper provides a basis for studying the nonlinear dynamic characteristics of the spur gear system as it experiences the gradual wear of teeth over time.
Removal of Acetaminophen from Aqueous Solutions in an Adsorption Process
Authors: Skwarczynska-Wojsa Agata , Puszkarewicz Alicja
Published: January 2024

Acetaminophen (C8H9NO2, also called paracetamol) is an active metabolite of phenacetin with antipyretic and analgesic effects and has been extensively used as a painkiller. Currently, the problem of pharmaceuticals in water and sewage is common, especially in highly urbanized countries. Laboratory-scale experiments were carried out using an adsorbent—granulated activated carbon (WD-extra)—to remove acetaminophen (ACT) from water. The initial concentration of acetaminophen was 20 mg ACT/dm3. The adsorption kinetics, influence of the pH on adsorption and dose of the used adsorbent were determined under batch conditions. The adsorption of ACT on activated carbon was more efficient when the water solution was acidic (at pH 2, it was the most effective). The highest percentage of removal (99%) was obtained for the WD-extra dose of 10.0 g/dm3. The time taken to establish the dynamic equilibrium of the system was 60 min. The effectiveness of adsorption was determined based on the Freundlich and Langmuir adsorption isotherms. It was found that WD-extra activated carbon effectively removed ACT from water solutions.
Modeling and Experimental Investigation of the Impact of the Hemispherical Tool on Heat Generation and Tensile Properties of Dissimilar Friction Stir Welded AA5083 and AA7075 Al Alloys
Authors: Essa Ahmed R. S. , Eldersy Ramy I. A. , Ahmed Mohamed M. Z. , Abd El-Aty Ali , Alamry Ali , Alzahrani Bandar , El-Nikhaily Ahmed E. , Habba Mohamed I. A.
Published: January 2024

This study investigated the effect of a hemispherical friction stir welding (FSW) tool on the heat generation and mechanical properties of dissimilar butt welded AA5083 and AA7075 alloys. FSW was performed on the dissimilar aluminum alloys AA5083-H111 and AA7075-T6 using welding speeds of 25, 50, and 75 mm/min. The tool rotation rate was kept constant at 500 rpm. An analytical model was developed to calculate heat generation and temperature distribution during the FSW process utilizing a hemispherical tool. The experimental results were compared to the calculated data. The latter confirms the accuracy of the analytical model, demonstrating a high degree of agreement. Sound FSW dissimilar joints were achieved at welding speeds of 50 and 25 mm/min. Meanwhile, joints created at a welding speed of 75 mm/min exhibited a tunnel-like defect, which can be attributed to the minimal heat generated at this particular welding speed. At a lower welding speed of 25 mm/min, a higher tensile strength of the dissimilar FSWed joints AA5083 and AA7075 was achieved with a joint efficiency of over 97%.
An Experimental and Numerical Study of the Laser Ablation of Bronze
Authors: Ghadiri Zahrani Esmaeil , Alexopoulou Vasiliki E. , Papazoglou Emmanouil L. , Azarhoushang Bahman , Markopoulos Angelos
Published: January 2024

The use of lasers in various precise material removal processes has emerged as a viable and efficient alternative to traditional mechanical methods. However, the laser ablation of materials is a complex, multi-parameter process where scanning paths need to be repeated multiple times. This repetition causes changes in the absorption and temperature distribution along the scanning path, thereby affecting the accuracy of the ablation. Therefore, it is crucial to thoroughly study these phenomena. This article presents an experimental and numerical study on the laser ablation of bronze (DIN: 1705) in a multi-track ablation process. Specifically, six consecutive passes using a ns laser at three different energy densities were conducted. After each pass, measurements of the ablation depth and pile-up height were taken at three distinct points along the track (start, middle, and end) to evaluate the efficiency and quality of the process. To gain a deeper understanding of the underlying physical mechanisms, a numerical simulation model based on the Finite Element Method (FEM) was developed. The effective absorptivity was defined through reverse engineering, and the material’s cooling rates were also estimated. This study’s findings provide significant insights into the influence of machining parameters on the ablation process and its progression with varying numbers of consecutive repetitions. A primarily linear correlation was deduced between the ablation depth, energy density, and number of repetitions, while the relationship with the pile-up height appeared to be more ambiguous and nonlinear. The estimated cooling rates ranged from 106 to 1010 [K/s]. Additionally, a heat accumulation phenomenon and a gradual temperature increase resulting from consecutive laser scans were also observed. A good agreement between the simulation results and experiments for the ablation depths was observed.
An Integrated YOLOv5 and Hierarchical Human-Weight-First Path Planning Approach for Efficient UAV Searching Systems
Authors: Chang Ing-Chau , Yen Chin-En , Chang Hao-Fu , Chen Yi-Wei , Hsu Ming-Tsung , Wang Wen-Fu , Yang Da-Yi , Hsieh Yu-Hsuan
Published: January 2024

Because the average number of missing people in our country is more than 20,000 per year, determining how to efficiently locate missing people is important. The traditional method of finding missing people involves deploying fixed cameras in some hotspots to capture images and using humans to identify targets from these images. However, in this approach, high costs are incurred in deploying sufficient cameras in order to avoid blind spots, and a great deal of time and human effort is wasted in identifying possible targets. Further, most AI-based search systems focus on how to improve the human body recognition model, without considering how to speed up the search in order to shorten the search time and improve search efficiency, which is the aim of this study. Hence, by exploiting the high-mobility characteristics of unmanned aerial vehicles (UAVs), this study proposes an integrated YOLOv5 and hierarchical human-weight-first (HWF) path planning framework to serve as an efficient UAV searching system, which works by dividing the whole searching process into two levels. At level one, a searching UAV is dispatched to a higher altitude to capture images, covering the whole search area. Then, the well-known artificial intelligence model YOLOv5 is used to identify all persons in the captured images and compute corresponding weighted scores for each block in the search area, according to the values of the identified human bodies, clothing types, and clothing colors. At level two, the UAV lowers its altitude to sequentially capture images for each block, in descending order according to its weighted score at level one, and it uses the YOLOv5 recognition model repeatedly until the search target is found. Two improved search algorithms, HWFR-S and HWFR-D, which incorporate the concept of the convenient visit threshold and weight difference, respectively, are further proposed to resolve the issue of the lengthy and redundant flight paths of HWF. The simulation results suggest that the HWF, HWFR-S, and HWFR-D search algorithms proposed in this study not only effectively reduce the length of a UAV’s search path and the number of search blocks but also decrease the search time required for a UAV to locate the search target, with a much higher search accuracy than the two traditional search algorithms. Moreover, this integrated YOLOv5 and HWF framework is implemented and tested in a real scenario to demonstrate its capability in enhancing the efficiency of a search and rescue operation.
Model Predictive Virtual Flux Control Method for Low Switching Loss Performance in Three-Phase AC/DC Pulse-width-Modulated Converters
Authors: Nguyen Minh Hoang , Kwak Sangshin , Choi Seungdeog
Published: January 2024

Three-phase AC/DC pulse-width-modulated (PWM) converters have been widely employed in various renewable energy systems and industrial applications, which require a high-efficiency power converter operation. This article proposes a technique to reduce switching loss in AC/DC converters by integrating a voltage vector preselection strategy to model predictive virtual flux control. The voltage vector preselection strategy preselects available voltage vectors corresponding to switching states that lead to minimum switching loss in the phase leg, which conducts the highest current. By using preselected voltage vectors, clamping intervals are generated at every fundamental period to maintain the present switching states of the power switches, resulting in the reduction in switching loss. Additionally, by using virtual flux control, the proposed approach can effectively be used under both ideal and distorted source voltage conditions. The proposed method is compared with the conventional model predictive current control and the conventional model predictive virtual flux control. Both a simulation and experiment are performed to validate the correctness and effectiveness of the proposed method, which has been found to decrease the switching loss of an AC/DC converter by up to 15% compared to conventional control schemes at a negligible increase in the input current total harmonic distortion and DC output voltage ripple.
Taxis-Driven Pattern Formation in Tri-Trophic Food Chain Model with Omnivory
Authors: Giricheva Evgeniya
Published: January 2024

The spatiotemporal dynamics of a three-component model of a food web are considered. The model describes the interactions between populations of resources, prey, and predators that consume both species. It assumes that the predator responds to the spatial change in the resource and prey densities by occupying areas where species density is higher (prey-taxis) and that the prey population avoids areas with a high predator density (predator-taxis). This work studies the conditions for the taxis-driven instability leading to the emergence of stationary patterns resulting from Turing instability and autowaves caused by wave instability. The existence of nonconstant positive steady states for the system is assessed through a rigorous bifurcation analysis. Meanwhile, the conditions for the existence of both types of instabilities are obtained by linear stability analysis. It is shown that the presence of cross-diffusion in the system supports the formation of spatially heterogeneous patterns. For low values of the resource-tactic and predator-tactic coefficients, Turing and wave instabilities coexist. The system undergoes only Turing instability for high levels of these parameters.
Research on the Route Choice Behavior of Urban Freight Vehicles Based on GPS Data
Authors: Zheng Lili , Gao Tian , Meng Lin , Ding Tongqiang , Chen Wenhao
Published: January 2024

In order to provide urban freight vehicles with navigation routes that better align with their travel preferences, it is necessary to analyze the patterns and characteristics of their route choices. This paper focuses on freight vehicles traveling within the city and examines their route selection behavior. Through an analysis of the GPS data collected from freight truck journeys in Changchun, China, this study outlines the characteristics of freight vehicle travel within the city. Variables that may influence their route selection behavior are defined as feature factors. The study employs the DBSCAN algorithm to identify the hotspots in origin–destination pairs for freight truck travel in Changchun. It also utilizes Breadth First Search Link Elimination to generate a set of route choices and constructs route selection behavior models based on Multinomial Logit and Path Size Logit. Based on the research findings, during navigation within the city road network, these vehicles exhibit a preference for side roads, a tendency to favor right turns at intersections, and a propensity to choose routes with lower duplication compared to alternative options. The study’s conclusions offer theoretical support for guiding urban freight vehicle routes and planning and managing freight traffic within the city.
MGATs: Motif-Based Graph Attention Networks
Authors: Sheng Jinfang , Zhang Yufeng , Wang Bin , Chang Yaoxing
Published: January 2024

In recent years, graph convolutional neural networks (GCNs) have become a popular research topic due to their outstanding performance in various complex network data mining tasks. However, current research on graph neural networks lacks understanding of the high-order structural features of networks, focusing mostly on node features and first-order neighbor features. This article proposes two new models, MGAT and MGATv2, by introducing high-order structure motifs that frequently appear in networks and combining them with graph attention mechanisms. By introducing a mixed information matrix based on motifs, the generation process of graph attention coefficients is improved, allowing the model to capture higher-order structural features. Compared with the latest research on various graph neural networks, both MGAT and MGATv2 achieve good results in node classification tasks. Furthermore, through various experimental studies on real datasets, we demonstrate that the introduction of network structural motifs can effectively enhance the expressive power of graph neural networks, indicating that both high-order structural features and attribute features are important components of network feature learning.
Exploring the Molecular Interaction of PCOS and Endometrial Carcinoma through Novel Hyperparameter-Optimized Ensemble Clustering Approaches
Authors: Karadayı Ataş Pınar
Published: January 2024

Polycystic ovary syndrome (PCOS) and endometrial carcinoma (EC) are gynecological conditions that have attracted significant attention due to the higher prevalence of EC in patients with PCOS. Even with this proven association, little is known about the complex molecular pathways that connect PCOS to an increased risk of EC. In order to address this, our study presents two main innovations. To provide a solid basis for our analysis, we have first created a dataset of genes linked to EC and PCOS. Second, we start by building fixed-size ensembles, and then we refine the configuration of a single clustering algorithm within the ensemble at each step of the hyperparameter optimization process. This optimization evaluates the potential performance of the ensemble as a whole, taking into consideration the interactions between each algorithm. All the models in the ensemble are individually optimized with the suitable hyperparameter optimization method, which allows us to tailor the strategy to the model’s needs. Our approach aims to improve the ensemble’s performance, significantly enhancing the accuracy and robustness of clustering outcomes. Through this approach, we aim to enhance our understanding of PCOS and EC, potentially leading to diagnostic and treatment breakthroughs.
Air Gap Fiber Bragg Grating for Simultaneous Strain and Temperature Measurement
Authors: Yang Fuling , Zhu Kehui , Yu Xiaoyi , Liu Tianze , Lu Ke , Wang Zelong , Li Yan
Published: January 2024

We propose an air gap fiber Bragg grating (g-FBG) sensor that can measure strain and temperature simultaneously. The sensor is made by aligning two fiber Bragg gratings (FBGs), and an air gap exists between these two sub-gratings. This sensor’s architecture allows it to form a spectrum with phase-shifted fiber Bragg grating (PSFBG) spectroscopy and Fabry–Perot interference (FPI) spectroscopy. Since the sensitivity of PSFBG and FPI spectra is different for strain and temperature, it is possible to measure both strain and temperature by measuring one of the reflected dips of PSFBG and the interference dip of FPI. The experimental results show that the strain sensitivity is about 11.95 pm/με via the dip wavelength detection of FPI, and the temperature sensitivity is about 9.64 pm/°C via the dip wavelength detection of PSFBG. The g-FBG sensor demonstrates a resolution of approximately ±3.7 με within the strain range of 0 to 1000 με and about ±0.6 °C within the temperature range of 25 °C to 120 °C. The proposed g-FBG sensor, characterized by its simple structure, compact size, and cost-effectiveness, exhibits significant potential in the field of multi-parameter measurements.
Promising Eco-Friendly Nanoparticles for Managing Bottom Rot Disease in Lettuce (Lactuca sativa var. longifolia)
Authors: Fetyan Nashwa A. H. , Essa Tarek A. , Salem Tamer M. , Taha Ahmed Aboueloyoun , Elgobashy Samah Fawzy , Tharwat Nagwa A. , Elsakhawy Tamer
Published: January 2024

Developing innovative, eco-friendly fungicide alternatives is crucial to mitigate the substantial threat fungal pathogens pose to crop yields. In this study, we assessed the in vitro effectiveness of SiO2, CuO, and γFe2O3 nanoparticles against Rhizoctonia solani. Furthermore, greenhouse experiments were conducted in artificially infested soil to evaluate the in vivo impact of nanoparticles under study. Two application methods were employed: soil drenching with 10 mL per pot at concentrations of 50, 100, and 200 mg L−1, and seedling dipping in nanoparticle suspensions at each concentration combined with soil drench. The combined treatment of 200 mg L−1 γFe2O3 or CuO nanoparticles showed the highest in vitro antifungal activity. Conversely, SiO2 nanoparticles demonstrated the lowest in vitro activity. Notably, the application of 200 mg/L SiO2 via the dipping and soil drenching methods decreased counts of silicate-solubilizing bacteria and Azospirillum spp. Whereas, application of 100 mg L−1 γFe2O3 nanoparticles via soil drenching increased soil bacterial counts, and CuO nanoparticles at 50 mg L−1 through dipping and soil drenching had the highest dehydrogenase value. γFe2O3 nanoparticles improved plant photosynthetic pigments, reduced malondialdehyde levels, and minimized membrane leakage in lettuce plants. A root anatomical study showed that 200 mg L−1 CuO nanoparticles induced toxicity, whereas 200 mg L−1 γFe2O3 or SiO2 nanoparticles positively affected root diameter, tissue structure, and various anatomical measurements in lettuce roots. γFe2O3 nanoparticles hold promise as a sustainable alternative for managing crop diseases.
Integration of Modified Solvay Process for Sodium Bicarbonate Synthesis from Saline Brines with Steelmaking for Utilization of Electric Arc Furnace Slag in CO2 Sequestration and Reagent Regeneration
Authors: Anto Shadman Monir , Ali Asif , Santos Rafael M.
Published: January 2024

In the pursuit of sustainable solutions for carbon dioxide CO2 sequestration and emission reduction in the steel industry, this study presents an innovative integration of steelmaking slag with the modified Solvay process for sodium bicarbonate (NaHCO3) synthesis from saline brines. Utilizing diverse minerals, including electric arc furnace (EAF) slag, olivine, and kimberlite, the study explored their reactivity under varied pH conditions and examined their potential in ammonium regeneration. SEM and WDXRF analyses were utilized to acquire morphological and chemical compositions of the minerals. Advanced techniques such as XRD and ICP-OES were employed to meticulously analyze mineralogical transformations and elemental concentrations. The findings demonstrate that steelmaking slag, owing to its superior reactivity and pH buffering capabilities, outperforms natural minerals. The integration of finer slag particles significantly elevated pH levels, facilitating efficient ammonium regeneration. Geochemical modeling provided valuable insights into mineral stability and reactivity, which aligned with the ICP-OES results. This synergistic approach not only aids in CO2 capture through mineral carbonation but also minimizes waste, showcasing its potential as a sustainable and environmentally responsible solution for CO2 mitigation in the steel industry.
Characterization and Cellular Toxicity Studies of Commercial Manganese Oxide Nanoparticles
Authors: Johnston Linda J. , Du Xiaomei , Zborowski Andre , Kennedy David C.
Published: January 2024

Manganese oxide nanoparticles (MnOx NPs) are finding applications in several environmentally important areas such as farming and energy storage. MnOx NPs span a range of metal oxidation states that open up a wide range of applications in catalysis as well. As a result, it is important to understand how such materials can impact human health through incidental exposure. In this study, we examined a range of commercially available Mn2O3 NPs and compared our characterization data to those supplied by manufacturers. Discrepancies were noted and then measured values were used to assess the biological impact of these materials on three mammalian cell lines—A549, HepG2 and J774A.1 cells. Cell toxicity assays showed that all Mn2O3 particles exhibited cytotoxic effects that may be correlated, at least in part, to the production of reactive oxygen species. All eight nanoforms also activated caspase 3 but not caspase 1, although the magnitude of these changes varied greatly between materials.
Elephantopus scaber L. Polysaccharides Alleviate Heat Stress-Induced Systemic Inflammation in Mice via Modulation of Characteristic Gut Microbiota and Metabolites
Authors: Wang Chen , Sun Dongfang , Deng Qi , Sun Lijun , Hu Lianhua , Fang Zhijia , Zhao Jian , Gooneratne Ravi
Published: January 2024

Elephantopus scaber L. (ESL) is a Chinese herb that is used both as a food and medicine, often being added to soups in summer in south China to relieve heat stress (HS), but its exact mechanism of action is unknown. In this study, heat-stressed mice were gavaged with ESL polysaccharides (ESLP) at 0, 150, 300, and 450 mg/kg/d−1 (n = 5) for seven days. The gut microbiota composition, short-chain fatty acids (SCFAs), seven neurotransmitters in faeces, expression of intestinal epithelial tight junction (TJ) proteins (Claudin-1, Occludin), and serum inflammatory cytokines were measured. The low dose of ESLP (ESLL) improved the adverse physiological conditions; significantly reduced the cytokines (TNF-α, IL-1β, IL-6) and lipopolysaccharide (LPS) levels (p < 0.05); upregulated the expression of Claudin-1; restored the gut microbiota composition including Achromobacter and Oscillospira, which were at similar levels to those in the normal control group; significantly increased beneficial SCFAs like butyric acid and 5-HT levels in the faeces of heat-stressed mice; and significantly decreased the valeric acid and glutamic acid level. The level of inflammatory markers significantly correlated with the above-mentioned indicators (p < 0.05). Thus, ESLL reduced the HS-induced systemic inflammation by optimizing gut microbiota (Achromobacter, Oscillospira) abundance, increasing gut beneficial SCFAs like butyric acid and 5-HT levels, and reducing gut valeric and glutamic acid levels.
Intestinal Oxalate Absorption, Enteric Hyperoxaluria, and Risk of Urinary Stone Formation in Patients with Crohn’s Disease
Authors: Siener Roswitha , Ernsten Charlotte , Speller Jan , Scheurlen Christian , Sauerbruch Tilman , Hesse Albrecht
Published: January 2024

Nephrolithiasis is a common urologic manifestation of Crohn’s disease. The purpose of this study was to investigate the clinical characteristics, intestinal oxalate absorption, and risk factors for urinary stone formation in these patients. In total, 27 patients with Crohn’s disease and 27 healthy subjects were included in the present study. Anthropometric, clinical, and 24 h urinary parameters were determined, and the [13C2]oxalate absorption test was performed. Among all patients, 18 had undergone ileal resection, 9 of whom had a history of urinary stones. Compared to healthy controls, the urinary excretion values of calcium, magnesium, potassium, sulfate, creatinine, and citrate were significantly lower in patients with Crohn’s disease. Intestinal oxalate absorption, the fractional and 24 h urinary oxalate excretion, and the risk of calcium oxalate stone formation were significantly higher in patients with urolithiasis than in patients without urolithiasis or in healthy controls. Regardless of the group, between 83% and 96% of the [13C2]oxalate was detected in the urine within the first 12 h after ingestion. The length of ileum resection correlated significantly with the intestinal absorption and urinary excretion of oxalate. These findings suggest that enteric hyperoxaluria can be attributed to the hyperabsorption of oxalate following extensive ileal resection. Oral supplementation of calcium and magnesium, as well as alkali citrate therapy, should be considered as treatment options for urolithiasis.
Food Intolerances, Food Allergies and IBS: Lights and Shadows
Authors: Pasta Andrea , Formisano Elena , Calabrese Francesco , Plaz Torres Maria , Bodini Giorgia , Marabotto Elisa , Pisciotta Livia , Giannini Edoardo , Furnari Manuele
Published: January 2024

This narrative review delves into the intricate relationship between irritable bowel syndrome (IBS) and food intolerances. IBS, a chronic functional gastrointestinal disorder, is characterized by symptoms like abdominal pain and altered bowel habits. The prevalence of IBS has increased globally, especially among young adults. Food and dietary habits play a crucial role in IBS management. About 85–90% of IBS patients report symptom exacerbation linked to specific food consumption, highlighting the strong connection between food intolerances and IBS. Food intolerances often exhibit a dose-dependent pattern, posing a challenge in identifying trigger foods. This issue is further complicated by the complex nature of gastrointestinal physiology and varying food compositions. This review discusses various dietary patterns and their impact on IBS, including the low-FODMAP diet, gluten-free diet, and Mediterranean diet. It highlights the importance of a personalized approach in dietary management, considering individual symptom variability and dietary history. In conclusion, this review emphasizes the need for accurate diagnosis and holistic management of IBS, considering the complex interplay between dietary factors and gastrointestinal pathophysiology. It underlines the importance of patient education and adherence to treatment plans, acknowledging the challenges posed by the variability in dietary triggers and the psychological impact of dietary restrictions.
Lactobacillus paracasei JY062 Alleviates Glucolipid Metabolism Disorders via the Adipoinsular Axis and Gut Microbiota
Authors: Su Yue , Ren Jing , Zhang Jingwen , Zheng Jiapeng , Zhang Qi , Tian Yueling , Zhang Yu , Jiang Yujun , Zhang Wei
Published: January 2024

Glycolipid metabolic disorders (GLMD) refer to a series of metabolic disorders caused by abnormal processes of glucose and lipid synthesis, decomposition, and absorption in the body, leading to glucose and lipid excess, insulin resistance, and obesity. Probiotic intervention is a new strategy to alleviate metabolic syndrome. Lactobacillus paracasei JY062 (L. paracasei JY062) was separated from the Tibet-fermented dairy products. The results demonstrated a strong ability to relieve blood glucose disorders, blood lipid disorders, and tissue damage. The LPH group had the best effect, significantly decreasing the total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), leptin, insulin, and free fatty acid (FFA) concentrations and increasing the high-density lipoprotein cholesterol, adiponectin, and GLP-1 level compared to HFD-group mice. L. paracasei JY062 could activate the APN-AMPK pathway, increased AdipoQ, AMPK GLUT-4, and PGC-1α mRNA expression and decreased SREBP-1c, ACC, and FAS mRNA expression. L. paracasei JY062 intervention decreased the relative abundance of harmful bacteria, increased the relative abundance of beneficial bacteria, and restored the imbalance of gut microbiota homeostasis caused by a high-glucose-fat diet. L. paracasei JY062 alleviated glucolipid metabolism disorders via the adipoinsular axis and gut microbiota. This study provided a theoretical basis for probiotics to ameliorate glucolipid metabolism disorders by regulating the adipoinsular axis.
Telenutrition Education Is Effective for Glycemic Management in People with Type 2 Diabetes Mellitus: A Non-Inferiority Randomized Controlled Trial in Japan
Authors: Mori Hiroyasu , Taniguchi Satoshi , Tamaki Yu , Tamaki Motoyuki , Akehi Yuko , Kuroda Akio , Matsuhisa Munehide
Published: January 2024

This study examined the non-inferior efficacy of telenutrition education compared with face-to-face nutrition education in managing glycemic control in people with type 2 diabetes mellitus (T2DM). Participants had T2DM and a glycated hemoglobin (HbA1c) ranged 6.5–9.5%. Thirty participants were randomly assigned to either the telenutrition or face-to-face nutrition education group. During the 32-week intervention period, the participants received four sessions on nutrition education from a registered dietitian at the hospital. The telenutrition group received remote education via a videoconferencing platform. Face-to-face nutrition education was conducted using paper-based instructions. The main outcome measure was the non-inferiority of HbA1c levels in the telenutrition group compared to the face-to-face nutrition group. The non-inferiority of telenutrition education was considered valid if the intergroup difference in the mean values of the change in HbA1c had a bilateral 95% confidence interval (CI) upper limit below 0.40%. The intergroup difference in the mean HbA1c change from baseline to the fourth nutrition education session was −0.11 (95% CI −0.54–0.32) for both groups. The upper limit of the bilateral 95% CI was 0.32%, which was below the 0.40% non-inferiority margin (non-inferiority test; p = 0.011). Telenutrition education was not inferior to face-to-face nutrition education for glycemic management in people with T2DM.
Polyamine Metabolism for Drug Intervention in Trypanosomatids
Authors: Pérez-Pertejo Yolanda , García-Estrada Carlos , Martínez-Valladares María , Murugesan Sankaranarayanan , Reguera Rosa M. , Balaña-Fouce Rafael
Published: January 2024

Neglected tropical diseases transmitted by trypanosomatids include three major human scourges that globally affect the world’s poorest people: African trypanosomiasis or sleeping sickness, American trypanosomiasis or Chagas disease and different types of leishmaniasis. Different metabolic pathways have been targeted to find antitrypanosomatid drugs, including polyamine metabolism. Since their discovery, the naturally occurring polyamines, putrescine, spermidine and spermine, have been considered important metabolites involved in cell growth. With a complex metabolism involving biosynthesis, catabolism and interconversion, the synthesis of putrescine and spermidine was targeted by thousands of compounds in an effort to produce cell growth blockade in tumor and infectious processes with limited success. However, the discovery of eflornithine (DFMO) as a curative drug against sleeping sickness encouraged researchers to develop new molecules against these diseases. Polyamine synthesis inhibitors have also provided insight into the peculiarities of this pathway between the host and the parasite, and also among different trypanosomatid species, thus allowing the search for new specific chemical entities aimed to treat these diseases and leading to the investigation of target-based scaffolds. The main molecular targets include the enzymes involved in polyamine biosynthesis (ornithine decarboxylase, S-adenosylmethionine decarboxylase and spermidine synthase), enzymes participating in their uptake from the environment, and the enzymes involved in the redox balance of the parasite. In this review, we summarize the research behind polyamine-based treatments, the current trends, and the main challenges in this field.
Retrospective Study on Genetic Diversity and Drug Resistance among People Living with HIV at an AIDS Clinic in Beijing
Authors: Shi Yan-Ze , Huang Hui-Huang , Wang Xin-Hua , Song Bing , Jiang Tian-Jun , Yu Min-Rui , Wang Ze-Rui , Li Rui-Ting , Jiao Yan-Mei , Su Xin , Wang Fu-Sheng
Published: January 2024

(1) Background: The objective of this study was to investigate the prevalence of genetic diversity and drug resistance mutations among people living with HIV (PLWH) attending clinics in Beijing. (2) Methods: A retrospective analysis was conducted on PLWH admitted to the Fifth Medical Center of People’s Liberation Army (PLA) General Hospital between 1 March 2013 and 31 July 2020. The participants were analyzed for pretreatment drug resistance (PDR) and acquired drug resistance (ADR). Nested polymerase chain reaction (PCR) was utilized to amplify the pol gene from plasma RNA samples obtained from the participants. Genotypic and HIV drug resistance were determined using the Stanford University HIV Drug Resistance Database. Univariate and multifactorial logistic analyses were used to assess the risk factors for PDR. (3) Results: The overall prevalence rates of PDR and ADR were 12.9% and 27.8%, respectively. Individuals treated with non-nucleoside reverse transcriptase inhibitors (NNRTIs) exhibited the highest prevalence of mutations. Specific mutation sites, such as V179D for NNRTIs and M184V and K65R for nucleoside reverse transcriptase inhibitors (NRTIs), were identified as prevalent mutations. Individuals treated with efavirenz (EFV) and nevirapine (NVP) were found to be susceptible to developing resistance. The multifactorial regression analyses indicated that the factors of circulating recombination form (CRF) genotype CRF07-BC and a high viral load were associated with an increased risk of PDR. CRF01-AE and CRF07-BC were the most prevalent HIV genotypes in our study. (4) Conclusions: The distribution of HIV genotypes in Beijing is complex. There is a need for baseline screening for HIV drug resistance among ART-naive individuals, as well as timely testing for drug resistance among ART-experienced individuals.
Anti-Inflammatory Activity of APPA (Apocynin and Paeonol) in Human Articular Chondrocytes
Authors: Fernández-Moreno Mercedes , Hermida-Gómez Tamara , Larkins Nicholas , Reynolds Alan , Blanco Francisco J.
Published: January 2024

Osteoarthritis (OA) is a chronic joint disease leading to cartilage loss and reduction in the joint space which results in pain. The current pharmacological treatment of OA is inadequate and pharmacological interventions focus on symptom management. APPA, a combination of apocynin (AP) and paeonol (PA), is a potential drug for treating OA. The aim of this study was to analyze the effects of APPA on the modulation of the inflammatory response in chondrocytes. Samples were incubated with IL-1β and APPA, and their responses to proinflammatory cytokines, catabolic mediators and redox responses were then measured. The effect of APPA on mitogenesis was also evaluated. Results show that APPA attenuated the expression of IL-8, TNF-α, MMP-3, MMP-13, SOD-2 and iNOS, resulting in the protection of human articular cartilage. APPA decreased PGC-1α gene expression induced by IL-1β. APPA did not modulate the gene expression of Mfn2, Sirt-1 or Sirt-3. The overall findings indicate that APPA may be an effective treatment for OA by targeting several of the pathways involved in OA pathogenesis.
Enhancing Oral Delivery of Biologics: A Non-Competitive and Cross-Reactive Anti-Leptin Receptor Nanofitin Demonstrates a Gut-Crossing Capacity in an Ex Vivo Porcine Intestinal Model
Authors: Masloh Solene , Chevrel Anne , Culot Maxime , Perrocheau Anaëlle , Kalia Yogeshvar N. , Frehel Samuel , Gaussin Rémi , Gosselet Fabien , Huet Simon , Zeisser Labouebe Magali , Scapozza Leonardo
Published: January 2024

Biotherapeutics exhibit high efficacy in targeted therapy, but their oral delivery is impeded by the harsh conditions of the gastrointestinal (GI) tract and limited intestinal absorption. This article presents a strategy to overcome the challenges of poor intestinal permeability by using a protein shuttle that specifically binds to an intestinal target, the leptin receptor (LepR), and exploiting its capacity to perform a receptor-mediated transport. Our proof-of-concept study focuses on the characterization and transport of robust affinity proteins, known as Nanofitins, across an ex vivo porcine intestinal model. We describe the potential to deliver biologically active molecules across the mucosa by fusing them with the Nanofitin 1-F08 targeting the LepR. This particular Nanofitin was selected for its absence of competition with leptin, its cross-reactivity with LepR from human, mouse, and pig hosts, and its shuttle capability associated with its ability to induce a receptor-mediated transport. This study paves the way for future in vivo demonstration of a safe and efficient oral-to-systemic delivery of targeted therapies.
Analysis of the Influence of Fabrication Errors on the Far-Field Performance of Si and Si3N4 Antennas
Authors: Xin Yifan , Liao Wenyuan , Yu Lei , Ma Pengfei , Wang Zheng , Yang Yibo , Chen Licheng , Wang Pengfei , Zhang Yejin , Pan Jiaoqing
Published: January 2024

Antennas are important components in optical phased arrays. However, their far-field performance deteriorates when random phase noise is introduced because of fabricating errors. For the first time, we use a finite-difference time-domain solution to quantitatively analyze the far-field characteristics of Si and Si3N4 antennas considering process errors. Under rough surface conditions based on a fishbone structure, we find that the quality of the main lobe of the Si antenna deteriorates badly, with −0.87 dB and −0.51 dB decreases in the sidelobe level and 5.78% and 3.74% deteriorations in the main peak power in the φ (phase-controlled) and θ (wavelength-controlled) directions, respectively. However, the Si3N4 antenna is only slightly impacted, with mere 0.39% and 0.71% deteriorations in the main peak power in the φ and θ directions, respectively, which is statistically about 1/15 of the Si antenna in the φ direction and 1/5 in the θ direction. The decreases in the sidelobe level are also slight, at about −0.08 dB and −0.01 dB, respectively. Furthermore, the advantages of the Si3N4 antenna become more remarkable with the introduction of random errors into the waveguide width and thickness. This work is of great significance for the design and optimization of OPA chips.
Genetic Variation and Evolutionary Analysis of Eggplant Mottled Dwarf Virus Isolates from Spain
Authors: Alfaro-Fernández Ana , Taengua Rafael , Font-San-Ambrosio Isabel , Sanahuja-Edo Esmeralda , Peiró Rosa , Galipienso Luis , Rubio Luis
Published: January 2024

The genetic variation and population structure of gene N (nucleocapsid) and part of gene L (replicase) from 13 eggplant mottle dwarf virus (EMDV) isolates from Spain were evaluated and compared with sequences of EMDV isolates from other countries retrieved from GenBank. Phylogenetic inference of part of gene L showed three main clades, one containing an EMDV isolate from Australia and the other two containing isolates from Iran and Europe, as well as four subclades. EMDV isolates from Spain were genetically very similar and grouped in a subclade together with one isolate from Germany and one from the UK. No new recombination events were detected in addition to one recombination previously reported, suggesting that recombination is rare for EMDV. The comparison of synonymous and non-synonymous rates showed that negative selection played an important role, and only two codons were under positive selection. Genetic differentiation (Fst test), phylogenetic and nucleotide diversity analyses suggest a unique introduction of EMDV to Spain and low gene flow with other countries. In contrast, Greece and Italy showed diverse populations with high gene flow between both.