bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of Vaccination Regimens on Immune Responses Using PED Replicon Vaccine: A Field Trial in PED-Negative and PED-Positive Thai Swine Farms
Authors: Sirisereewan Chaitawat , Nguyen Thanh Che , Iampraphat Nanthiya , Lin Hongyao , Ellerma Leonardo , Sirithanyakul Pisit , Kedkovid Roongtham , Thanawongnuwech Roongroje
Published: January 2025

Porcine epidemic diarrhea virus (PEDV) is an economically important pathogen of swine, causing severe diarrhea in neonates with high morbidity and mortality. Vaccination is a key strategy for PEDV control, but optimizing regimens based on herd status is essential for improving immunity and protection. This study evaluated immune responses to different vaccination protocols using a PED replicon vaccine (PED-RP) in Thai swine farms with varying PED statuses. Two field trials were conducted: Experiment 1 (n = 120) on a PED-negative farm used a killed vaccine followed by PED-RP, while Experiment 2 (n = 42) on a PED-positive farm used a modified-live vaccine (MLV) followed by PED-RP. In Experiment 1, gilts boosted with PED-RP during gestation showed significantly higher colostrum IgA levels compared to those receiving two doses of the killed vaccine. In Experiment 2, priming with MLV followed by PED-RP resulted in increased neutralizing antibody titers and seropositivity, outperforming the MLV-only regimen after acclimatization. These findings suggest that using PED-RP as a booster can enhance serum and lactogenic immunity, providing a promising strategy for improving immune responses in both PED-negative and PED-positive herds. The study highlights the importance of tailored vaccination strategies to optimize herd immunity and protect piglets from PEDV.
Effects of Dietary Iron Levels on the Production Performance, Nutrient Digestibility, Blood Biochemistry, and Meat and Fur Quality of Growing Rex Rabbits
Authors: Chen Jiali , Wang Jiyuan , Liu Lei , Li Fuchang
Published: January 2025

The objective of this study was to explore the effects of dietary iron (Fe) levels on the production performance, nutrient digestibility, blood biochemistry, and meat and fur quality of growing Rex rabbits. Two hundred 3-month-old Rex rabbits were randomly allocated to five groups, each with forty replicates. Rabbits were fed a basal diet supplemented with varying levels of Fe (0, 20, 40, 80, and 160 mg/kg) in the form of ferrous sulfate monohydrate. The analyzed Fe concentrations in the diets were 8.2, 25.4, 49.1, 85.6, and 178.7 mg/kg, respectively. Over a 35-day trial period, rabbits supplemented with 40 mg/kg Fe showed a markedly increased average daily feed intake, average daily gain, and fur area compared to the control group (p < 0.05). The addition of 20 and 40 mg/kg Fe markedly improved the digestibility of crude protein and nitrogen (p < 0.05). Additionally, 80 mg/kg Fe supplementation significantly increased the redness of the longissimus dorsi muscle 30 min postmortem (p < 0.05). Dietary Fe addition also significantly elevated serum concentrations of Fe and copper (p < 0.05) while decreasing the total Fe-binding capacity (p < 0.05). In conclusion, dietary Fe supplementation boosted growth performance, protein and nitrogen digestibility, Fe stores, and meat and fur quality in Rex rabbits. Specifically, 40 mg/kg Fe (diet Fe content of 49.1 mg/kg) improved growth performance, nutrient digestibility, and fur quality, while 80 mg/kg Fe (diet Fe content at 85.6 mg/kg) was optimal for enhancing meat quality.
Application of Genomic Selection in Beef Cattle Disease Prevention
Authors: Kasimanickam Ramanathan , Ferreira Joao Carlos Pinheiro , Kastelic John , Kasimanickam Vanmathy
Published: January 2025

Genomic applications in beef cattle disease prevention have gained traction in recent years, offering new strategies for improving herd health and reducing economic losses in the livestock industry. Advances in genomics, including identification of genetic markers linked to disease resistance, provide powerful tools for early detection, selection, and management of cattle resistant to infectious diseases. By incorporating genomic technologies such as whole-genome sequencing, genotyping, and transcriptomics, researchers can identify specific genetic variants associated with resistance to pathogens like bovine respiratory disease and Johne’s disease. These genomic insights allow for more accurate breeding programs aimed at enhancing disease resistance and overall herd resilience. Genomic selection, in particular, enables identification of individuals with superior genetic traits for immune function, reducing the need for antibiotic treatments and improving animal welfare. Moreover, precision medicine, powered by genomic data, supports development of tailored health management strategies, including targeted vaccination plans and antimicrobial stewardship. Incorporation of genomic tools in beef cattle management also offers the potential for early disease detection, facilitating proactive interventions that reduce the spread of infections. Despite challenges like cost, data interpretation and integration into current management systems, the potential advantages of genomic applications in disease prevention are substantial. As these technologies advance, they are anticipated to have crucial roles in improving sustainability (by enhancing herd performance), profitability (by improving overall herd longevity), and biosecurity (by decreasing the likelihood of disease outbreaks) of beef cattle production systems worldwide.
Estimation of Optimal and Maximum Standardized Ileal Digestible Methionine Requirements Based on Performance for Male Broilers Aged 0 to 21 Days
Authors: An Su-Hyun , Kong Changsu
Published: January 2025

Two experiments were conducted with Ross 308 male broiler chickens to determine the standardized ileal digestible (SID) methionine (Met) requirement for maximum weight gain during the starter (0 to 10 days) and grower (10 to 21 days) phases. A total of 720 and 288 birds were allocated to Experiments 1 and 2, respectively, and assigned six dietary treatments in a randomized complete block design. The experimental diets were formulated with corn, soybean meal, and synthetic amino acids to achieve varying SID Met levels (3.8 to 5.8 g/kg in experiment 1 and 3.0 to 5.5 g/kg in experiment 2), with increments of 0.4 and 0.5 g/kg. Weight gain, feed intake, and the gain-to-feed ratio (G:F) increased quadratically (p < 0.05) as dietary SID Met increased. The SID Met requirements for maximum weight gain and G:F in the starter phase were 5.1 g/kg (79.5 mg/d) and 5.3 g/kg (89.8 mg/d), while in the grower phase, they were 5.5 g/kg (343.0 mg/d) and 5.4 g/kg (330.3 mg/d), respectively. These findings, divided into two growth phases, provide guidelines to facilitate the optimal and maximum performance of young male broilers, and this may facilitate the selection of appropriate values for formulating broiler feed. These findings also highlight the importance of Met supplementation strategies for improving performance.
Development of a Portable Rapid Detection Method for Porcine Epidemic Diarrhea Virus Using Reverse Transcription-Recombinase-Aided Amplification Technology
Authors: Zhao Yiran , Yi Weijie , Yang Qicheng , Li Jiahao , Shan Yanke , Liu Fei
Published: January 2025

Porcine epidemic diarrhea virus (PEDV) continues to spread globally, causing clinical symptoms in piglets, including watery diarrhea, vomiting, and dehydration. Its exceptionally high morbidity and mortality rate contributes significantly to the economic losses of the swine industry. The continuous genetic mutations of PEDV have compromised the effectiveness of classical strain vaccines. Early detection and accurate diagnosis are therefore crucial for controlling its further spread. Developing a detection method that is user-friendly, highly sensitive, and efficient is crucial for disease control. In this study, a point-of-care rapid detection method for PEDV was successfully established using reverse transcription-recombinase-aided amplification (RT-RAA) technology. This method enables results to be obtained within 20 min of amplification at a constant temperature of 42 °C. It demonstrates high sensitivity, with a detection limit as low as 1 copy/μL, and shows strong specificity, with no cross-reactivity observed with seven other common swine pathogens. When applied to clinical samples, the results were 100% consistent with those obtained by RT-qPCR. This method is distinguished by its portable instrumentation and simple operation, making it particularly suitable for resource-constrained settings.
Management and Outcome of Invasive Clindamycin-Resistant MRSA Community-Associated Infections in Children
Authors: Macias Amanda E. , Stimes Grant , Kaplan Sheldon L. , Vallejo Jesus G. , Hulten Kristina G. , McNeil J. Chase
Published: January 2025

Background: Clindamycin resistance among community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) complicates the management of a challenging infection. Little data exist to guide clinicians in the management of invasive clindamycin-resistant CA-MRSA infections in children and studies using oral regimens such as trimethoprim-sulfamethoxazole (TMP-SMX) and linezolid for treatment of these infections are limited. We sought to reevaluate antibiotic management among invasive CA-MRSA at a tertiary children’s hospital. Methods: Cases of invasive clindamycin-resistant MRSA infections in children were identified through an ongoing S. aureus surveillance study. Eligible cases were those occurring in otherwise healthy children from 2011–2021. Medical records were reviewed. Results: Thirty-four subjects met inclusion criteria. The most common diagnoses were osteomyelitis (n = 17) and deep abscess (n = 7). The median duration of IV therapy was 11.5 days (IQR 6–42 days) and total therapy (IV + oral) was 32 days (IQR 23–42). Overall, 50% of patients were transitioned to oral therapy. Definitive antibiotics used for treatment included vancomycin (n = 15), TMP/SMX (n = 9), linezolid (n = 7), ceftaroline (n = 2), and doxycycline (n = 1). Cure rates were similar across definitive antibiotic therapies (vancomycin-73.3%; TMP/SMX-88.9%; ceftaroline 50%; linezolid and doxycycline-100%). Three subjects died of MRSA disease; two definitively treated with vancomycin and one with ceftaroline. Conclusions: Vancomycin is the most commonly used agent in the treatment of invasive clindamycin-resistant CA-MRSA in children at our center. However, TMP/SMX and linezolid can be considered as oral options when completing treatment in select cases. Further work is needed to evaluate the optimal management of these infections.
Reduced Glutathione Promoted Growth Performance by Improving the Jejunal Barrier, Antioxidant Function, and Altering Proteomics of Weaned Piglets
Authors: Tian Zhimei , Cui Yiyan , Yu Miao , Deng Dun , Li Zhenming , Ma Xianyong , Qu Mingren
Published: January 2025

Reduced glutathione (GSH) is a main nonenzymatic antioxidant, but its effects and underlying mechanisms on growth and intestinal health in weaned piglets still require further assessment. A total of 180 weaned piglets were randomly allotted to 5 groups: a basal diet (CON), and a basal diet supplemented with antibiotic chlortetracycline (ABX), 50 (GSH1), 65 (GSH2), or 100 mg/kg GSH (GSH3). Results revealed that dietary GSH1, GSH2, and ABX improved body weight and the average daily gain of weaned piglets, and ABX decreased albumin content but increased aspartate aminotransferase (AST) activity and the ratio of AST to alanine transaminase levels in plasma. GSH2 significantly decreased glucose content but increased the content of triglyceride and cholesterol in the plasma. Both GSH1 and GSH2 improved the jejunal mucosa architecture (villus height, crypt depth, and the ratio of villus height to crypt depth), tight junction protein (ZO-1 and Occludin), and antioxidant capacity (CAT and MDA), and the effects were superior to ABX. Dietary GSH improved the jejunal barrier by probably inhibiting the myosin light chain kinas pathway to up-regulate the transcript expression of tight junction protein (ZO-1 and Occludin) and Mucins. Through the proteomics analysis of the jejunal mucosa using 4D-DIA, the KEGG pathway enrichment analysis showed that differentiated proteins were significantly enriched in redox homeostasis-related pathways such as glutathione metabolism, cytochrome P450, the reactive oxygen species metabolic pathway, the oxidative phosphorylation pathway, and the phosphatidylinositol 3-kinase-serine/threonine kinase pathway in GSH2 vs. CON and in GSH2 vs. ABX. The results of proteomics and qRT-PCR showed that GSH supplementation might dose-dependently promote growth performance and that it alleviated the weaning stress-induced oxidative injury of the jejunal mucosa in piglets by activating SIRTI and Akt pathways to regulate GPX4, HSP70, FoxO1. Therefore, diets supplemented with 50–65 mg/kg GSH can promote the growth of and relieve intestinal oxidative injury in weaned piglets.
Targeting Mitochondrial Dysfunction in Cerebral Ischemia: Advances in Pharmacological Interventions
Authors: Belenichev Igor , Popazova Olena , Bukhtiyarova Nina , Ryzhenko Victor , Pavlov Sergii , Suprun Elina , Oksenych Valentyn , Kamyshnyi Oleksandr
Published: January 2025

The study of mitochondrial dysfunction has become increasingly pivotal in elucidating the pathophysiology of various cerebral pathologies, particularly neurodegenerative disorders. Mitochondria are essential for cellular energy metabolism, regulation of reactive oxygen species (ROS), calcium homeostasis, and the execution of apoptotic processes. Disruptions in mitochondrial function, driven by factors such as oxidative stress, excitotoxicity, and altered ion balance, lead to neuronal death and contribute to cognitive impairments in several brain diseases. Mitochondrial dysfunction can arise from genetic mutations, ischemic events, hypoxia, and other environmental factors. This article highlights the critical role of mitochondrial dysfunction in the progression of neurodegenerative diseases and discusses the need for targeted therapeutic strategies to attenuate cellular damage, restore mitochondrial function, and enhance neuroprotection.
Protective Effect of Conditioned Medium of Immortalized Human Stem Cells from Exfoliated Deciduous Teeth Against Hair Graying Caused by X-Ray Irradiation via Its Antioxidative Activity
Authors: Katahira Yasuhiro , Horio Eri , Yamaguchi Natsuki , Sonoda Jukito , Yamagishi Miu , Miyakawa Satomi , Murakami Fumihiro , Hasegawa Hideaki , Mizoguchi Izuru , Yoshimoto Takayuki
Published: January 2025

Hair graying is one of the common visible signs of human aging, resulting from decreased or abolished melanogenesis due to the depletion of melanocyte stem cells through excess accumulation of oxidative stress. Cell-free therapy using a conditioned medium (CM) of mesenchymal stem cells has been highlighted in the field of regenerative medicine owing to its potent therapeutic effects with lower regulatory hurdles and safety risk. Recently, we demonstrated that a CM of an immortalized stem cell line from human exfoliated deciduous teeth (SHED) has protective effects against a mouse model of ulcer formation via antioxidative and angiogenic activities mediated by HGF and VEGF. However, to date, no effective treatments for hair graying have been developed, and the effect of SHED-CM on hair graying remains unknown. In this study, we have investigated the effect of SHED-CM on a hair graying mouse model caused by X-ray irradiation. Repetitive subcutaneous administrations of SHED-CM greatly suppressed the development of hair graying, when compared to control medium, resulting in reduced cutaneous expression of 8-hydroxy-2′-deoxyguanosine, the major product of DNA damage induced by reactive oxygen species. Consistent with these in vivo results, SHED-CM significantly inhibited the cell death caused by X-ray irradiation in melanoma cell line B16F10 cells. Immunodepletion of HGF or VEGF in the SHED-CM revealed that this inhibition was due to suppression of the generation of reactive oxygen species, which was mainly mediated by HGF and probably VEGF. These results suggest that SHED-CM has protective effects against hair graying via its antioxidative activity.
Dietary Tannic Acid Promotes Growth Performance and Resistance Against Aeromonas hydrophila Infection by Improving the Antioxidative Capacity and Intestinal Health in the Chinese Soft-Shelled Turtle (Pelodiscus sinensis)
Authors: Ji Liqin , Shangguan Yisen , Chen Chen , Wei Chengqing , Zhu Junxian , Hong Xiaoyou , Liu Xiaoli , Zhu Xinping , Li Wei
Published: January 2025

To investigate the effect of tannic acid (TA) on the growth, disease resistance, and intestinal health of Chinese soft-shelled turtles, individual turtles were fed with 0 g/kg (CG), 0.5 g/kg, 1 g/kg, 2 g/kg, and 4 g/kg TA diets for 98 days. Afterwards, the turtles’ disease resistance was tested using Aeromonas hydrophila. The results showed that 0.5–4 g/kg of dietary TA increased the growth performance and feed utilization (p < 0.05), with 2.38 g/kg being the optimal level for the specific growth rate (SGR). The addition of 0.5–4 g/kg of TA in diets increased the mucosal fold height and submucosa thickness of the small intestine, which reached a maximum of 2 g/kg. The addition of 0.5–2 g/kg of TA effectively reduced the cumulative mortality that had been induced by A. hydrophila, with the 2 g/kg dosage leading to the lowest mortality. Additionally, 1–4 g/kg of TA improved the T-SOD, CAT, and GSH-Px activities during infection, while 2 g/kg of dietary TA enhanced the richness and diversity of the microbiota, for example, by increasing Actinobacteria but inhibiting Firmicutes. The transcriptome demonstrated that the predominant differentially expressed genes (DEGs) in TA2 were mainly enriched in the PPAR signaling pathway (Acsl5, Apoa2, Apoa5, Fabp1, Fabp2, and Fabp6); in glycine, serine, and threonine metabolism (Chdh, Gatm, and Shmt1); and in steroid biosynthesis (Cel, Hsd17b7, Soat2, and Sqle). The main differentially expressed metabolites (DEMs) that were discovered by means of metabolome analysis included cholylhistidine, calcipotriol, 13-O-tetradecanoylphorbol 12-acetate, and hexahomomethionine in CG vs. TA2. Integrative analyses of two omics revealed that 2 g/kg of TA mitigated inflammation by activating the PPAR signaling pathway and regulating the lipid metabolism via multiple pathways, such as steroid biosynthesis and α-linolenic acid metabolism. In general, the inclusion of 2 g/kg of TA in turtle diets can optimally promote growth and bacterial resistance by maintaining intestinal health and improving antioxidant capacity.
Exploring Strategies to Prevent and Treat Ovarian Cancer in Terms of Oxidative Stress and Antioxidants
Authors: Long Yanru , Shi Houhui , Ye Jiatian , Qi Xiaorong
Published: January 2025

Oxidative stress is a state of imbalance between the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS) and the antioxidant defence system in the body. Oxidative stress may be associated with a variety of diseases, such as ovarian cancer, diabetes mellitus, and neurodegeneration. The generation of oxidative stress in ovarian cancer, one of the common and refractory malignancies among gynaecological tumours, may be associated with several factors. On the one hand, the increased metabolism of ovarian cancer cells can lead to the increased production of ROS, and on the other hand, the impaired antioxidant defence system of ovarian cancer cells is not able to effectively scavenge the excessive ROS. In addition, chemotherapy and radiotherapy may elevate the oxidative stress in ovarian cancer cells. Oxidative stress can cause oxidative damage, promote the development of ovarian cancer, and even result in drug resistance. Therefore, studying oxidative stress in ovarian cancer is important for the prevention and treatment of ovarian cancer. Antioxidants, important markers of oxidative stress, might serve as one of the strategies for preventing and treating ovarian cancer. In this review, we will discuss the complex relationship between oxidative stress and ovarian cancer, as well as the role and therapeutic potential of antioxidants in ovarian cancer, thus guiding future research and clinical interventions.
The Role of Extracts of Edible Parts and Production Wastes of Globe Artichoke (Cynara cardunculus L. var. scolymus (L.)) in Counteracting Oxidative Stress
Authors: Laghezza Masci Valentina , Mezzani Irene , Alicandri Enrica , Tomassi William , Paolacci Anna Rita , Covino Stefano , Vinciguerra Vittorio , Catalani Elisabetta , Cervia Davide , Ciaffi Mario , Garzoli Stefania , Ovidi Elisa
Published: January 2025

In addition to the immature edible flower heads, the cultivation of globe artichoke (Cynara cardunculus L. var. scolymus (L.) Fiori) generates substantial quantities of by-products, including leaves, stems, and roots, which constitute potential sources of bioactive compounds and prebiotic dietary fiber. Preserving agricultural biodiversity and promoting socioeconomic development are essential for enhancing domestic production and fostering innovation. In the search for new biomolecules with antioxidant properties, this research focused on a globe artichoke landrace at risk of genetic erosion, still cultivated in the northern part of the Lazio region, known as the “Carciofo Ortano”. To investigate the antioxidant properties of various globe artichoke tissues from the “Carciofo Ortano” landrace, methanolic extracts were prepared from the immature main and secondary flower heads, stems, and leaves of representative genotypes of this landrace. Additionally, extracts were obtained from the same tissues of four landraces/clones included in the varietal platform of the PGI “Carciofo Romanesco del Lazio”, which served as reference genotypes: Campagnano, Castellammare, C3, and Grato 1. The antioxidant properties of these extracts were assessed using FRAP, ABTS, DPPH assays, and total phenolic content (TPC). The stem and secondary flower head extracts of two representative “Carciofo Ortano” genotypes and the Grato 1 clone, which have higher phenolic content, demonstrated the highest antioxidant activity. These extracts were therefore studied for their chemical profile using HPLC-DAD and SPME-GC/MS analysis. Additionally, the same extracts were investigated in vitro for their antioxidant capacity in differentiated SH-SY5Y cells, assessing their effects on ROS levels and the restoration of GSH levels. Furthermore, the in vivo beneficial effects of counteracting oxidative stress were evaluated in high sucrose-fed Drosophila melanogaster, as oxidative stress is a typical hallmark of hyperglycemic status. Overall, the results indicated that the edible immature inflorescences of the “Carciofo Ortano” landrace, along with the byproducts of its cultivation, are sources of raw materials containing biomolecules whose properties can be exploited for further applications in the pharmaceutical and medical sectors.
State-of-the-Art Detection and Diagnosis Methods for Rolling Bearing Defects: A Comprehensive Review
Authors: Sun Bojun , Sheng Zixin , Song Peng , Sun Huilin , Wang Fei , Sun Xiaogang , Liu Junyan
Published: January 2025

Rolling bearings are essential transmission and support components in aircraft engines, playing a critical role in ensuring their safe and stable operation. Rolling bearing faults have a significant impact and should not be ignored. The effective diagnosis of bearing faults has always been a critical requirement for ensuring reliable operation. With the increasing demands of modern manufacturing to reduce costs and improve quality, the development of advanced bearing fault detection methods has become indispensable. This paper presents the brief review of recent trends in research on bearing failure modes, bearing fault detection and diagnosis methods, and development trends and prospects. This article provides a comprehensive review of the existing fault diagnosis methods for rolling bearings in four aspects: the integration of advanced sensor technology and advanced data processing technology, multimodal fusion, intelligent detection, and real-time monitoring. It discusses methods based on vibration analysis, acoustic methods, current-based methods, electromagnetic methods, infrared methods, radiographic methods, visual methods, and intelligent detection methods. This study reveals that the application of intelligent detection technology, multimodal fusion detection technology, and real-time online monitoring technology has achieved favorable results. In the future, bearing fault detection will develop in a more intelligent, integrated, and real-time direction.
Viable and Sustainable Model for Adoption of New Technologies in Industry 4.0 and 5.0: Case Study on Pellet Manufacturing
Authors: Solano García Pavel , Ramírez-Gutiérrez Ana Gabriela , Morales Matamoros Oswaldo , Coria Páez Ana Lilia
Published: January 2025

This manuscript presents the development and testing of a novel model designed to help organizations, particularly small and medium-sized enterprises (SMEs), address the challenges of integrating new technologies within the frameworks of Industry 4.0 and 5.0. The proposed model is a metamodel that evaluates organizational and contextual vulnerabilities concerning both existing technologies and potential external technologies under consideration for adoption. It synthesizes three foundational frameworks: the Viable System Model (VSM), the principles of viable and sustainable systems, and the Technology, Organization, and Environment (TOE) Model. The findings demonstrate the practical applicability of this model in an SME context, showcasing its ability to facilitate the gradual and sustainable adoption of new technologies. By aligning business needs with technological solutions and leveraging insights from computer science and organizational cybernetics, the model adapts to varying levels of technological adoption, integrating organizational dynamics and business evolution to support the implementation of emerging technologies.
Polynomial Exact Schedulability and Infeasibility Test for Fixed-Priority Scheduling on Multiprocessor Platforms
Authors: Garanina Natalia , Anureev Igor , Kondratyev Dmitry
Published: January 2025

In this paper, we develop an exact schedulability test and sufficient infeasibility test for fixed-priority scheduling on multiprocessor platforms. We base our tests on presenting real-time systems as a Kripke model for dynamic real-time systems with sporadic non-preemptible tasks running on a multiprocessor platform and an online scheduler using global fixed priorities. This model includes states and transitions between these states, allows us to formally justify a polynomial-time algorithm for an exact schedulability test using the idea of backward reachability. Using this algorithm, we perform the exact schedulability test for the above real-time systems, in which there is one more task than the processors. The main advantage of this algorithm is its polynomial complexity, while, in general, the problem of the exact schedulability testing of real-time systems on multiprocessor platforms is NP-hard. The infeasibility test uses the same algorithm for an arbitrary task-to-processor ratio, providing a sufficient infeasibility condition: if the real-time system under test is not schedulable in some cases, the algorithm detects this. We conduct an experimental study of our algorithms on the datasets generated with different utilization values and compare them to several state-of-the-art schedulability tests. The experiments show that the performance of our algorithm exceeds the performance of its analogues while its accuracy is similar.
Analytical Methods for Atmospheric Carbonyl Compounds: A Review
Authors: Gao Xiaoshuai , Zhang Xin , Nie Yan , Bao Jiemeng , Li Junling , Gao Rui , Li Yunfeng , Wei Wei , Yan Xiaoyu , Yan Yongxin , Li Hong
Published: January 2025

Atmospheric carbonyl compounds have significant impacts on the atmospheric environment and human health, making the selection of appropriate analytical techniques crucial for accurately detecting these compounds in specific environments. Based on extensive literature research, this study summarized the development history, relevant features, and applicable scenarios of the main analytical techniques for atmospheric carbonyl compounds; pointed out the main problems and challenges in this field; and discussed the needs and prospects of future research and application. It was found that the direct sampling methods of atmospheric carbonyl compounds were applicable to low-molecular-weight carbonyl species with low reactivity, low boiling points, high polarity, and high volatility, while indirect sampling methods were suitable for a wider range and various types and phases of species. For formaldehyde, offline detection was primarily influenced by chemical reagents and reaction conditions, whereas online monitoring relied on sufficiently stable operating environments. For multiple carbonyl compounds, offline detection results were greatly influenced by detectors coupled with chromatography, whereas online monitoring techniques were applicable to all types of volatile organic compounds (VOCs), including some carbonyl compounds, providing higher temporal resolution and improved isomer identification with the development of online mass spectrometry. The combined use of proton transfer reaction-mass spectrometry (PTR-MS) and liquid chromatography-mass spectrometry (GC-MS) was suitable for the detection of carbonyl compounds in atmospheric photochemical smog chamber simulation studies. Currently, offline analytical techniques for carbonyl compounds require significant time and advanced experimental skills for multiple optimization experiments to detect a broader range of species. Online monitoring techniques face challenges such as poor stability and limited species coverage. In smog chamber simulation studies, the detection of carbonyl compounds is heavily influenced by both the sampling system and the chamber itself. Future efforts should focus on improving the environmental adaptability and automation of carbonyl compound analytical techniques, the synergistic use of various techniques, developing new sampling systems, and reducing the impact of the chamber itself on carbonyl compound detection, in order to enhance detection sensitivity, selectivity, time resolution, accuracy, and operability.
Temporal and Spatial Assessment of Glacier Elevation Change in the Kangri Karpo Region Using ASTER Data from 2000 to 2024
Authors: Wang Qihua , Yang Yuande , Hu Jiayu , Zhang Jianglong , Li Zuqiang , Wang Yuechen
Published: January 2025

Temperate glaciers in the Kangri Karpo region of the southeastern Qinghai–Tibet Plateau (QTP) have experienced significant ablation in recent decades, increasing the risk of glacier-related hazards and impacting regional water resources. However, the spatial and temporal pattern of mass loss in these glaciers remains inadequately quantified. In this study, we used ASTER L1A stereo images to construct a high-resolution elevation time series and provide a comprehensive spatial–temporal assessment of glacier elevation change from 2000 to 2024. The results indicate that almost all glaciers have experienced rapid ablation, with an average surface elevation decrease of −18.35 ± 5.13 m, corresponding to a rate of −0.76 ± 0.21 m yr−1. Glaciers in the region were divided into the northern and southern basins, with average rates of −0.79 ± 0.17 m yr−1 and −0.72 ± 0.13 m yr−1, respectively. A notable difference in acceleration trends between the two basins was observed, with the elevation rate increasing from −0.78 ± 0.17m yr−1 to −1.04 ± 0.17 m yr−1 and from −0.52 ± 0.13 m yr−1 to −0.92 ± 0.13 m yr−1, respectively. The seasonal cycle was identified in glacier surface elevation change, with an accumulation period from November to March followed by a prolonged ablation period. The seasonal amplitude decreased with elevation, with higher elevations exhibiting longer accumulation periods and less ablation. Correlation analysis with meteorological data indicated that higher summer temperatures and increased summer rainfall intensify elevation loss, while increased spring snowfall may reduce ablation. Our analysis highlights distinct variations in glacier elevation changes across different locations, elevations, and climatic conditions in the Kangri Karpo region, providing valuable insights into glacier responses to environmental changes on the Tibetan Plateau.
Can Hearing Aids Improve Physical Activity in Adults with Hearing Loss? A Feasibility Study
Authors: Goodwin Maria V. , Slade Katelynn , Kingsnorth Andrew P. , Urry Emily , Maidment David W.
Published: January 2025

Background/Objectives: Adults with hearing loss demonstrate poorer overall health outcomes (e.g., physical health, cognitive functioning and wellbeing) and lower levels of physical activity/function compared to those without hearing loss. Hearing aids have the potential to improve cognitive and wellbeing factors, but there is a dearth of evidence on their impact on physical health outcomes. Evidence on the association between hearing aid provision and physical activity is mostly limited to cross-sectional studies. This research aimed to assess whether a study can be performed to identify whether the provision of hearing aids can improve physical activity. Methods: This study employed a preregistered observational (prospective cohort) study design of ten older adults (51–75 years) completed assessments at baseline and again at a six-week follow-up. The participants wore an accelerometer (ActiGraph GT9X) without feedback for the full duration of the study. Feasibility was determined using pre-defined criteria, including study drop-out, adherence to accelerometer use and willingness. A battery of health outcomes was also assessed at baseline and follow-up. Conclusions: Overall, this study was perceived favourably, with all participants reporting that they enjoyed taking part. Participant retention was 100%, and adherence to the wrist-worn accelerometers was “good” (70%). However, recruitment was challenging, and some participants found the accelerometers to be burdensome. Descriptive statistics for all outcome measures showed non-significant changes in the expected direction (e.g., improved physical activity, cognition and wellbeing). Although the study was well received by participants, modifications to the recruitment strategy and activity tracking procedures are necessary before future large-scale trials assessing the effectiveness of hearing aids on physical activity can be undertaken.
Composites Based on Poly(ortho-toluidine) and WS2 Sheets for Applications in the Supercapacitor Field
Authors: Burlanescu Teodora , Smaranda Ion , Androne Andreea , Florica Cristina Stefania , Cercel Madalina , Paraschiv Mirela , Udrescu Adelina , Lőrinczi Adam , Palade Petru , Galatanu Andrei , Negrila Catalin , Matei Elena , Dinescu Monica , Cercel Radu , Baibarac Mihaela
Published: January 2025

In this work, three methods for the synthesis of composites based on poly(ortho-toluidine) (POT) and WS2 are reported: (a) the solid-state interaction (SSI) of POT with WS2 nanoparticles (NPs); (b) the in situ chemical polymerization (ICP) of ortho-toluidine (OT); and (c) the electrochemical polymerization (ECP) of OT. The preparation of WS2 sheets was performed by the ball milling of the WS2 NPs followed by ultrasonication in the solvent N,N’-dimethyl formamide. During the synthesis of the POT/WS2 composites by SSI and ICP, an additional exfoliation of the WS2 NPs was reported. In this work, we demonstrated the following: (a) the ICP method leads to POT/WS2 composites, which contain repeating units of POT in the leucoemeraldine salt (LS) state, while (b) the ECP method leads to POT/WS2 composites, which contain repeating units of POT in the emeraldine salt (ES) state. Capacitances equal to 123.5, 465.76, and 751.6 mF cm−2 in the cases of POT-ES/WS2 composites, synthesized by SSI, ICP, and ECP, respectively, were reported.
Cognitive Method for Synthesising a Fuzzy Controller Mathematical Model Using a Genetic Algorithm for Tuning
Authors: Vladov Serhii
Published: January 2025

In this article, a fuzzy controller mathematical model synthesising method that uses cognitive computing and a genetic algorithm for automated tuning and adaptation to changing environmental conditions has been developed. The technique consists of 12 stages, including creating the control objects’ mathematical model and tuning the controller coefficients using classical methods. The research pays special attention to the error parameters and their derivative fuzzification, which simplifies the development of logical rules and helps increase the stability of the systems. The fuzzy controller parameters were tuned using a genetic algorithm in a computational experiment based on helicopter flight data. The results show an increase in the integral quality criterion from 85.36 to 98.19%, which confirms an increase in control efficiency by 12.83%. The fuzzy controller use made it possible to significantly improve the helicopter turboshaft engines’ gas-generator rotor speed control performance, reducing the first and second types of errors by 2.06…12.58 times compared to traditional methods.
Impact of Seasonal Atmospheric Factors and Photoperiod on Floral Biology, Plant–Pollinator Interactions, and Plant Reproduction on Turnera ulmifolia L. (Passifloraceae)
Authors: Layek Ujjwal , Das Nandita , Samanta Arabinda , Karmakar Prakash
Published: January 2025

Reproductive traits and plant–pollinator interactions largely depend on seasonal weather conditions, which are species-specific. Turnera ulmifolia is an ornamental plant distributed worldwide. There is little information about plant species’ reproductive ecology and environmental factors’ impact on it. Here, we aimed to examine the effects of seasonal atmospheric factors (e.g., temperature, light, relative humidity, rainfall) and photoperiod on flowering, interactions with flower visitors, and the reproductive success of Turnera ulmifolia in West Bengal, India. Flowering intensity peaked in hot summers and dropped in cold winters, correlating positively with temperature and humidity. Flower opening and closing occurred earlier on hot days, while flower longevity increased in winter, showing a negative correlation with temperature and humidity. Pollen and ovule production were lower in cold weather, positively linked to temperature and humidity. The self-compatible plant was moderately dependent on pollinators and had no pollination deficit in open conditions. Visitor abundance, richness, and diversity varied season-wise, with higher values during spring–summer. Based on pollinating agents, the plant showed multiple pollination modes (e.g., melittophily, myophily, myrmecophily, and psychophily). Effective pollinators were Amegilla zonata, Borbo cinnara, Halictus acrocephalus, Nomia (Curvinomia) strigata, and Tetragonula iridipennis. The fruit set (%) did not differ significantly season-wise, but the seed set remained higher in the hot days of summer than in cold winter. Therefore, it can be concluded that atmospheric factors and photoperiod significantly impact floral traits, plant–pollinator interactions, and plant reproduction.
Transcriptomic-Based Identification of miR-125a Novel Targets in Human Hepatocarcinoma Cells
Authors: De Leo Ilenia , Mosca Nicola , Pezzullo Mariaceleste , Valletta Danila , Manfrevola Francesco , Mele Vincenza Grazia , Chianese Rosanna , Russo Aniello , Potenza Nicoletta
Published: January 2025

Hepatocellular carcinoma (HCC) is among the most aggressive and lethal human tumors. Many functional studies have demonstrated the role of non-coding RNAs (ncRNA), particularly microRNAs (miRNA), in the regulation of hepatocarcinogenesis driving pathways. MiR-125a-5p (miR-125a) has been consistently reported as an oncosuppressive miRNA, as demonstrated in vivo and in vitro. However, its HCC relevant targets and molecular mechanisms are still largely unknown. Here, a genome-wide perspective of the whole miR-125a targetome has been achieved. In particular, two different HCC cell lines were subjected to a miRNA boosting by mimic transfections, and consequently many genes were de-regulated, as observed by a transcriptomic approach. The merging of down-regulated genes with results from bioinformatic predictive tools yielded a number of candidate direct targets that were further experimentally validated by luciferase-based reporter assays. Different novel targets were found, in particular ARID3A, CCNJ, LIPA, NR6A1, and NUP210, oncogenes in various tumors and here also related to HCC through miR-125a regulation. The RNA interactions investigated in this work could pave the way to piece together the RNA regulatory networks governed by the miRNA impacting on hepatocarcinogenesis, and be exploited in the future for identifying novel biomarkers and therapeutic targets in HCC.
Effects of Carbon Dioxide Therapy on Skin Wound Healing
Authors: Prazeres José , Lima Ana , Ribeiro Gesiane
Published: January 2025

Promoting rapid healing is a concern in skin wound treatment, as the increased pain and the loss of functional ability when wounds become chronic create a complex problem to manage. This scoping review aimed to explore the literature and synthesize existing knowledge on the therapeutic use of CO2 in treating cutaneous wounds. The literature was selected using previously defined inclusion and exclusion criteria, and 22 articles were selected for data extraction. The most researched type of injury was chronic wounds located on the extremities of the limbs. Carboxytherapy was performed in five different ways: subcutaneous, intradermal, or intralesional injections; in hot water baths with temperatures ranging from 30 to 42 °C; transcutaneous application; intra-abdominal insufflation; and a paste for transcutaneous local application. The main effects of CO2 therapy described were as follows: improved blood flow and local oxygenation, reduction of the inflammatory process, increased collagen production, and improved clinical aspects of wounds, with faster healing. Carboxytherapy can be considered a good alternative for treating skin wounds, although further studies should be pursued to elucidate its molecular mechanisms and enhance its efficacy.
Mendelian Randomization Reveals Potential Causal Relationships Between DNA Damage Repair-Related Genes and Inflammatory Bowel Disease
Authors: Qi Zhihao , Li Quan , Yang Shuhua , Fu Chun , Hu Burong
Published: January 2025

DNA damage repair (DDR) plays a key role in maintaining genomic stability and developing inflammatory bowel disease (IBD). However, no report about the causal association between DDR and IBD exists. Whether DDR-related genes are the precise causal association to IBD in etiology remains unclear. Herein, we employed a multi-omics summary data-based Mendelian randomization (SMR) approach to ascertain the potential causal effects of DDR-related genes in IBD. Methods: Summary statistics from expression quantitative trait loci (eQTL), DNA methylation QTL (mQTL), and protein QTL (pQTL) on European descent were included. The GWAS summarized data for IBD and its two subtypes, Crohn’s disease (CD) and ulcerative colitis (UC), were acquired from the FinnGen study. We elected from genetic variants located within or near 2000 DDR-related genes in cis, which are closely associated with DDR-related gene changes. Variants were selected as instrumental variables (IVs) and assessed for causality with IBD and its subtypes using both SMR and two-sample MR (TSMR) approaches. Colocalization analysis was employed to evaluate whether a single genetic variant simultaneously influences two traits, thereby validating the pleiotropy hypothesis. Results: We identified seven DDR-related genes (Arid5b, Cox5a, Erbb2, Ube2l3, Gpx1, H2bcl2, and Mapk3), 33 DNA methylation genes, and two DDR-related proteins (CD274 and FCGR2A) which were all causally associated with IBD and its subtypes. Beyond causality, we integrated the multi-omics data between mQTL-eQTL and conducted druggability values. We found that DNA methylation of Erbb2 and Gpx1 significantly impacted their gene expression levels offering insights into the potential regulatory mechanisms of risk variants on IBD. Meanwhile, CD247 and FCGR2A could serve as targets for potential pharmacological interventions in IBD. Conclusions: Our study demonstrates the causal role of DDR in IBD based on the data-driven MR. Moreover, we found potential regulatory mechanisms of risk variants on IBD and potential pharmacological targets.
Baicalin Mitigates Cardiac Hypertrophy and Fibrosis by Inhibiting the p85a Subunit of PI3K
Authors: He Lu , Zhu Min , Yin Rui , Dai Liangli , Chen Juan , Zhou Jie
Published: January 2025

Background: Heart failure (HF) is a serious public health concern. Baicalin is one of the major active ingredients of a traditional Chinese herbal medicine, Huang Qin, which is used to treat patients with chest pain or cardiac discomfort. However, the underlying mechanism(s) of the cardioprotective effect of baicalin are still not fully understood. Methods: Isoprenaline injection or transverse aortic constriction-induced animal models and isoprenaline or angiotensin 2 administration-induced cell models of heart failure were established. Baicalin (15 mg/kg/day or 25 mg/kg/day) was administered in vivo, and 10 μM baicalin was administered in vitro. Potential pharmacological targets of baicalin and genes related to heart failure were identified via different databases, which suggested that PI3K–Akt may be involved in the effects of baicalin. Molecular docking was carried out to reveal the effect of baicalin on p85a. Results: We observed significant antihypertrophic and antifibrotic effects of baicalin both in vivo and in vitro. The mean cross-sectional area of cardiomyocytes recovered from 390 μm2 in the HF group to 195 μm2 in the baicalin-treated group. The area of fibrosis was reduced from 2.8-fold in the HF group to 1.62-fold in the baicalin-treated group. Baicalin displayed a significant cardioprotective effect via the inhibition of the PI3K signaling pathway by binding with five amino acid residues of the p85a regulatory subunit of PI3K. The combination treatment of baicalin and an inhibitor of PI3K p110 demonstrated a stronger cardioprotective effect. The mean ejection fraction increased from 54% in the baicalin-treated group to 67% in the combination treatment group. Conclusions: Our work identified baicalin as a new active herbal ingredient that is able to treat isoprenaline-induced heart dysfunction and suggests that p85a is a pharmacological target. These findings reveal the significant potential of baicalin combined with an inhibitor of PI3K p110 for the treatment of heart failure and support more clinical trials in the future.