bluuebunny/crossref_metadata_2025 huggingface dataset:
Molecular epidemiology and diagnostics of KRAS mutations in human cancer
Authors: Timar Jozsef , Kashofer Karl
Published: July 2020

RAS mutation is the most frequent oncogenic alteration in human cancers. KRAS is the most frequently mutated followed by NRAS. The emblematic KRAS mutant cancers are pancreatic, colorectal, lung adenocarcinomas and urogenital cancers. KRAS mutation frequencies are relatively stable worldwide in various cancer types with the one exception of lung adenocarcinoma. The frequencies of KRAS variant alleles appears cancer type specific, reflecting the various carcinogenic processes. In addition to point mutation KRAS, allelic imbalances are also frequent in human cancers leading to the predominance of a mutant allele. KRAS mutant cancers are characterized by typical, cancer-type-specific co-occurring mutations and distinct gene expression signatures. The heterogeneity of KRAS mutant primary cancers is significant, affecting the variant allele frequency, which could lead to unpredictable branching development in metastases. Selection of minute mutant subclones in the primary tumors or metastases during target therapies can also occur frequently in lung or colorectal cancers leading to acquired resistance. Ultrahigh sensitivity techniques are now routinely available for diagnostic purposes, but the proper determination of mutant allele frequency of KRAS in the primary or metastatic tissues may have larger clinical significance.
Cognitive-Behavioral Therapy for Envy
Authors: Leahy Robert L.
Published: July 2020

Envy is a ubiquitous social emotion often associated with depression, hostility and shame. Often confused with jealousy which involves the fear or anger that a primary relationship is threatened by a third party, envy is an emotion focused on threats to status such that another person’s “gain” is viewed as a “loss” for the self. There is very little in the cognitive behavioral literature on the nature and treatment of envy. In this article I outline the research on the nature of envy, the evolutionary model of envy, the relevance of an integrative CBT model that draws on Beck’s Generic Cognitive Model and Emotional Schema Therapy, and the implications for case conceptualization and treatment.
Assessment and management of effects of large hydropower projects on aquatic ecosystems in British Columbia, Canada
Authors: Bradford Michael J.
Published: July 2020

Most of the hydropower generated in Canada’s western province of British Columbia is generated by a small number of large storage or diversion projects that impound large rivers. All but one were built between 1950 and 1985; a period when environmental considerations for large projects were evolving to present-day social, political and regulatory standards. Large projects result in ecosystem transformations; river valleys are converted to reservoirs, and the release of water for power generation results in highly altered flow regimes downstream of dams. I describe the effects of three projects on aquatic ecosystems and measures that have been taken over the past 60 years to monitor and mitigate those effects, with an emphasis on downstream effects to fish populations. I briefly review methods that were used to predict effects, particularly on key fish species, and consider the role of adaptive management and its alternatives on resolving uncertainties about ecological effects of large hydroelectric projects.
Postoperative respiratory state assessment using the Integrated Pulmonary Index (IPI) and resultant nurse interventions in the post-anesthesia care unit: a randomized controlled trial
Authors: Broens Suzanne J. L. , Prins Susan A. , de Kleer Dorinne , Niesters Marieke , Dahan Albert , van Velzen Monique
Published: July 2020

Although postoperative adverse respiratory events, defined by a decrease in respiratory rate (RR) and/or a drop in oxygen saturation (SpO2), occur frequently, many of such events are missed. The purpose of the current study was to assess whether continuous monitoring of the integrated pulmonary index (IPI), a composite index of SpO2, RR, end-tidal PCO2 and heart rate, alters our ability to identify and prevent adverse respiratory events in postoperative patients. Eighty postoperative patients were subjected to continuous respiratory monitoring during the first postoperative night using RR and pulse oximetry and the IPI monitor. Patients were randomized to receive intervention based on standard care (observational) or based on the IPI monitor (interventional). Nurses were asked to respond to adverse respiratory events with an intervention to improve the patient’s respiratory condition. There was no difference in the number of patients that experienced at least one adverse respiratory event: 21 and 16 in observational and interventional group, respectively (p = 0.218). Compared to the observational group, the use of the IPI monitor led to an increase in the number of interventions performed by nurses to improve the respiratory status of the patient (average 13 versus 39 interventions, p < 0.001). This difference was associated with a significant reduction of the median number of events per patient (2.5 versus 6, p < 0.05) and a shorter median duration of events (62 s versus 75 s, p < 0.001). The use of the IPI monitor in postoperative patients did not result in a reduction of the number of patients experiencing adverse respiratory events, compared to standard clinical care. However, it did lead to an increased number of nurse interventions and a decreased number and duration of respiratory events in patients that experienced postoperative adverse respiratory events.
Hayek on complexity, uncertainty and pandemic response
Authors: Pennington Mark
Published: July 2020

This paper draws on Hayek’s distinction between simple and complex phenomena to understand the nature of the challenge facing policymakers in responding to the new coronavirus pandemic. It shows that while government action is justifiable there may be few systemic mechanisms that enable policymakers to distinguish better from worse policy responses, or to make such distinctions in sufficient time. It then argues that this may be a more general characteristic of large-scale public policy making procedures and illustrates the importance of returning to a market-based political economy at the earliest convenience.
Fabrication and electrical characteristics of flash-sintered SiO2-doped ZnO-Bi2O3-MnO2 varistors
Authors: Peng Pai , Deng Yujun , Niu Jingpeng , Shi Liyi , Mei Yunzhu , Du Sanming , Liu Juan , Xu Dong
Published: July 2020

The dense ZnO-Bi2O3-MnO2-xSiO2 (ZBMS) varistors for x = 0, 1, 2, 3 wt% were fabricated by flash sintering method under the low temperature of 850 °C within 2 min. The sample temperature was estimated by a black body radiation model in the flash sintering process. The crystalline phase assemblage, density, microstructure, and electrical characteristics of the flash-sintered ZBMS varistors with different SiO2-doped content were investigated. According to the XRD analysis, many secondary phases were detected due to the SiO2 doping. Meanwhile, the average grain size decrease with increasing SiO2-doped content. The improved nonlinear characteristics were obtained in SiO2-doped samples, which can be attributed to the ion migration and oxygen absorption induced by the doped SiO2. The flash-sintered ZBMS varistor ceramics for x = 2 wt% exhibited excellent comprehensive electrical properties, with the nonlinear coefficient of 24.5, the threshold voltage and leakage current of 385 V·mm−1 and 11.8 µA, respectively.
MoS2 nanoparticles grown on carbon nanomaterials for lubricating oil additives
Authors: Gong Kuiliang , Lou Wenjing , Zhao Gaiqing , Wu Xinhu , Wang Xiaobo
Published: July 2020

In this study, the nanocomposites of MoS2 nanoparticles (NPs) grown on carbon nanotubes (MoS2@CNT), graphene (MoS2@Gr), and fullerene C60 (MoS2@C60) were synthesized, characterized, and evaluated for potential use as lubricant additives. By using the benefit of the synergistic effect between MoS2 and carbon nanomaterials (CNMs), these nanocomposites can be well dispersed in polyalkylene glycol (PAG) base oil and show superior stability compared with pure MoS2 NPs. Moreover, the dispersions of MoS2@CNT, MoS2@Gr, and MoS2@C60 added in PAG have noticeably improved friction reducing and antiwear (AW) behaviors at elevated temperature for comparison with that of PAG and PAG containing CNT, Gr, C60, and MoS2 NPs, respectively. The enhanced lubricating properties of these nanocomposites were also elucidated by exploring the tribofilm formed on the disc.
Fractionation of coal through organo-separative refining for enhancing its potential for the CO2-gasification
Authors: Dhawan Heena , Kumar Rohit , Upadhyayula Sreedevi , Pant K. K. , Sharma D. K.
Published: July 2020

Coal gasification has already been extensively studied earlier under varying conditions of steam, CO2, O2, inert conditions. Belbaid coal and its e, N and NMP-DETA SCC products recovered through organo-refining under milder ambient pressure conditions were subjected to CO2-gasification in a fixed bed reactor under varying conditions. CO2 being an inert gas becomes the most challenging to be utilized during the gasification process. The SCCs showed better CO2-gasification reactivity than the raw Belbaid coal at 900 °C. The use of the catalyst K2CO3 tremendously increased the gasification reactivity for both raw coal and the SCCs. The use of sugarcane bagasse for CO2-gasification along with raw coal as well as with residual coal was also studied. Gasification under CO2 atmosphere conditions was used to structurally understand the coals as the coal structure gets loosened after extraction.
Adaptive Newton-Type Schemes Based on Projections
Authors: Amrein Mario , Hilber Norbert
Published: July 2020

In this work we present and discuss a possible globalization concept for Newton-type methods. We consider nonlinear problems $$f(x)=0$$

f
(
x
)
=
0

in $${\mathbb {R}}^{n}$$

R

n

using the concepts from ordinary differential equations as a basis for the proposed numerical solution procedure. Thus, the starting point of our approach is within the framework of solving ordinary differential equations numerically. Accordingly, we are able to reformulate general Newton-type iteration schemes using an adaptive step size control procedure. In doing so, we derive and discuss a discrete adaptive solution scheme, thereby trying to mimic the underlying continuous problem numerically without losing the famous quadratic convergence regime of the classical Newton method in a vicinity of a regular solution. The derivation of the proposed adaptive iteration scheme relies on a simple orthogonal projection argument taking into account that, sufficiently close to regular solutions, the vector field corresponding to the Newton scheme is approximately linear. We test and exemplify our adaptive root-finding scheme using a few low-dimensional examples. Based on the presented examples, we finally show some performance data.
Global Constitutionalism and Democracy: the Case of Colombia
Authors: Thornhill Chris , de Araújo Calabria Carina Rodrigues
Published: July 2020

Focusing on the case of Colombia, this article sets out a sociological examination of constitutions marked by strong, activist judiciaries, by entrenched systems of human rights protection, and by emphatic implementation of global human rights law. Contra standard critiques of this constitutional model, it argues that such constitutions need to be seen as creating a new pattern of democracy, which is often distinctively adapted to structures in societies in which the typical patterns of legitimation and subject formation required for democratic government were obstructed. In polities with such constitutions, legal institutions and norm setters have at times assumed the status offunctional equivalentsfor more typical democratically mandated actors and institutions. In such polities, further, global law assumes essential importance as it creates new sources of normative authorization for legislation and stimulates new lines of articulation between government and society. The article concludes that analysis of such polities, exemplified by Colombia, shows that the common categories of democratic-constitutional analysis are no longer always adequate for understanding current tendencies in democratic formation, and they can easily undermine democracy itself.
4547 Understanding the molecular mechanism of natural killer cell deficiency to improve natural killer cell in vitro differentiation for therapeutics
Authors: Schmit Megan , Baxley Ryan , Mace Emily , Orange Jordan , Miller Jeffery , Bielinsky Anja-Katrin
Published: July 2020

OBJECTIVES/GOALS: Natural killer (NK) cells are a potential cancer therapeutic but expanding NK cells efficiently in vitro is difficult. Natural killer cell deficiency (NKD), a primary immune deficiency affecting only NK cells, is caused by defects in several DNA replication proteins. By studying NKD we will achieve better NK cell in vitro differentiation. METHODS/STUDY POPULATION: One patient with NKD has a compound heterozygous mutation in the essential DNA replication protein MCM10. We hypothesize that in individuals with NKD, dramatic telomere erosion from abnormal DNA replication leads to premature senescence and the loss of NK cells. To test our hypothesis, we will knockout one allele of MCM10 or over express MCM10 in NK cells isolated from blood. We will then monitor telomere length, expansion and cytotoxic activity of these NK cells. To understand the role of MCM10 in early stages of NK cell development we will deplete MCM10 in induced pluripotent stem cells and differentiate these cells into NK cells. During this differentiation we will monitor progression through NK cell developmental stages as well as telomere length and senescence markers. RESULTS/ANTICIPATED RESULTS: Telomeres insulate chromosomes and induce permanent growth arrest (senescence) when they are critically short. We have demonstrated that depletion of a DNA replication protein causes telomere erosion and increases senescence markers. NK cells have shorter telomeres and lower telomerase expression than other immune cells. We predict, this relatively poor telomere maintenance sensitizes NK cells to telomere loss upon depletion of replication proteins. During in vitro differentiation, we expect NK cell precursors to undergo premature senescence secondary to telomere shortening. Furthermore, we expect supplementation of DNA replication proteins will enhance NK cell expansion and maturation. DISCUSSION/SIGNIFICANCE OF IMPACT: NKD patients have provided the scientific community with clues as to what proteins NK cells rely on for their development. This project aims not only to understand why these proteins are critical, but to harness that information for cellular anti-cancer therapeutics.
4347 Bimodal Visual-Olfactory Training for Post-Surgical or Post-Traumatic Olfactory Dysfunction (VOLT Trial)
Authors: Peterson Andrew Michael , Kallogjeri Dorina , Piccirillo Jay
Published: July 2020

OBJECTIVES/GOALS: 1) Assess the patient-reported, perceived change in olfactory function after bimodal visual-olfactory training (OT) 2) Assess change in olfactory function after bimodal visual-olfactory training with a smell identification test 3) Assess which scents are most important to people with olfactory dysfunction (OD) METHODS/STUDY POPULATION: The participants are adults with subjective or clinically diagnosed OD with post-surgical or traumatic etiologies within the last 5 years. At the first of two study visits, participants complete the University of Pennsylvania Smell Identification Test (UPSIT) and complete general health (SF-36) and olfactory-related quality-of-life questionnaires. From a list of 34 scents, participants chose the 4 scents most important to them and smelled the scents twice daily for 3 months. Olfactory testing and the quality-of-life questionnaires were repeated at the final visit. RESULTS/ANTICIPATED RESULTS: 10 participants have enrolled in the study. There was one screen fail and one withdrawal. Six participants are currently undergoing OT and two have completed the study. Seven participants have post-surgical etiology and three have post-traumatic etiology of their OD. Of the two participants who have completed the study, one had an UPSIT score improvement from 25 to 33 out of the 40 questions correct. The minimally clinically important difference on the UPSIT is 4. She reports improvement subjectively. The second participant had a UPSIT score change from 25 to 24 and reports ability to smell is neither better nor worse. DISCUSSION/SIGNIFICANCE OF IMPACT: Traumatic and post-surgical, particularly post-transphenoidal hypophysectomy, are common etiologies of OD and no effective treatments exist. The results from our pilot study will help better inform the best way to undergo OT, how effective it is, and the planning of future studies.
4188 Consistent Differences in Lumbar Spine Alignment Between Low Back Pain Subgroups and Sexes during Clinical and Functional Sitting Tests
Authors: Hooker Quenten L , Lanier Vanessa M. , Van Dillen Linda R.
Published: July 2020

OBJECTIVES/GOALS: Test the validity of a system for subgrouping people with CLBP by comparing lumbar spine alignment in two CLBP subgroups and sexes during clinical tests of maximum flexed and extended sitting and a functional test of preferred sitting. METHODS/STUDY POPULATION: Using the Movement System Impairment classification system, 154 participants with CLBP were subgrouped based on the predominant direction of altered movement and alignment patterns and symptoms during a standardized examination. Participants performed a functional test of preferred sitting followed by clinical tests of maximum flexed and extended sitting in random order. Reflective markers were place superficial to T12, L3 and S1 spinous processes. 3D marker co-ordinate data were collected using an 8 camera motion capture system. Sagittal plane lumbar curvature angle (LCA), defined as the angular distance between T12, L3, and S1 landmarks was calculated for each test. A three-way mixed effect ANOVA model was used to examine the following effects: test, subgroup, sex, test*subgroup, test*sex, subgroup*sex. RESULTS/ANTICIPATED RESULTS: Test: Lumbar alignment patterns were different for flexed [LCA = 7.4° (6.1, 8.7)], extended [LCA = −22.6° (−23.9,−21.3)], and preferred [LCA = −3.8° (−5.2,−2.5)] sitting tests. LBP subgroup: Rotation-extension [LCA = −7.5° (−8.7,−6.3)] had more extended lumbar alignment than rotation [LCA = −5.2° (−6.2,−4.2)]. Sex: Women had more extended lumbar alignment [LCA = −10.3° (−11.2,−9.3)] than men [LCA = −2.5° (−3.7,−1.2)]. Test*sex: The difference in lumbar alignment between women and men was smaller during the flexed sitting test [women = 4.2° (2.5, 5.9), men = 9.9° (7.8, 12.1)], compared to extended [women = −27.5° (−29.2, −25.8), men = −17.0° (−9.2, −14.8)] and preferred [women = −7.4° (−9.1, −5.8), men = −0.3° (−2.5, 1.8)]. The test*subgroup (p = 0.84) and subgroup*sex (p = 0.87) interactions were not significant.
4211 Longitudinal cohort study of the association between atopic dermatitis and depression/anxiety throughout childhood
Authors: Kern Chloe E , Lewinn Kaja , Wan Joy , Abuabara Katrina
Published: July 2020

OBJECTIVES/GOALS: Atopic dermatitis is one of the most common chronic childhood conditions worldwide and is associated with poor mental health outcomes. Our aim is to determine whether childhood atopic dermatitis is associated with symptoms of depression throughout childhood and adolescence, and whether this association is mediated by serum inflammatory markers. METHODS/STUDY POPULATION: We will perform a longitudinal analysis of over 7000 children from an existing prospective cohort. The primary exposure is atopic dermatitis (AD) annual period prevalence measured by a standardized questionnaire at 12 time points between age 6 months and 16 years. Depression is measured using self-reported responses to the Short Moods and Feelings Questionnaire at 6 time points between 10 and 18 years of age. Cross-sectional regression analyses will be performed to compare depressive signs between children with and without AD and test for dose-response effects with AD and depression. Longitudinal analyses will be conducted using mixed-effects models to estimate the average effect across childhood. We will complete a mediation analysis to determine the extent to which IL-6 and CRP mediate this association. RESULTS/ANTICIPATED RESULTS: We anticipate that atopic dermatitis will be associated with SMFQ scores in a dose response relationship, and that inflammatory markers CRP and IL-6 will partly mediate this association. DISCUSSION/SIGNIFICANCE OF IMPACT: Childhood is a critical time for mental health. Understanding the longitudinal relationship between atopic dermatitis, depression, and inflammatory mediators is crucial as new biologic treatments targeting inflammatory cascades are approved for atopic dermatitis and have the potential to prevent mental health conditions.
4433 SGRQ score is associated with treatment status for patients with non-tuberculous mycobacterial lung disease
Authors: Garcia Bryan , Grady Abigail , Christon Lilian , Flume Patrick , Dorman Susan
Published: July 2020

OBJECTIVES/GOALS: The Saint Georges Respiratory Questionnaire (SGRQ) is used as a patient reported outcome tool for clinical research in COPD and bronchiectasis. We established a registry and biospecimen repository of bronchiectasis patients with and without NTM and report associations between clinical phenotype and SGRQ scores. METHODS/STUDY POPULATION: Patients were recruited in a cross-sectional format from the Bronchiectasis, Cystic Fibrosis, and NTM clinics at our institution. All patients provided at least one sputum sample in the six months prior to inclusion. Clinical and epidemiologically relevant data was obtained, and blood specimens were processed and preserved. Patients were grouped based on clinical phenotype and differences in SGRQ scores were analyzed using ANOVA or Student’s t-test. Descriptive statistics are reported as means and standard deviations, p<0.05 considered significant. RESULTS/ANTICIPATED RESULTS: 72 NTM patients completed the SGRQ including 39 patients not on treatment (Colonized), 29 patients on NTM directed antibiotics, and 4 patients whose infection was cured in the past year. Among patients on treatment, 14 were treatment refractory (positive cultures beyond 12 months of therapy). The mean age of all NTM patients was 59.5±17.6 and 80.5% were female. Mean SGRQ Total scores were significantly higher among patients receiving treatment compared to patients considered colonized (35.7± 22.0 colonized group versus 48.8± 15.8 treatment group, p = 0.011). The SGRQ subdomain scores including Impacts (26.2± 26.2 colonized group versus 42.5± 17.0 treatment group, p = 0.01) and Activities (41.7± 31.8 colonized group versus 59.3± 24.5 treatment group, p = 0.018) were also significantly different between groups. DISCUSSION/SIGNIFICANCE OF IMPACT: We developed a cross sectional cohort of NTM patients and assessed associations between clinical phenotype and SGRQ score. Preliminary data suggests that female sex, treatment status, and therapeutic duration are associated with higher SGRQ scores. We intend to continue to assess the potential for specific SGRQ questions to be used for quantifying disease symptom severity for NTM patients.
4038 Development of a Catheter Stabilization Device for Stent Placement Aid
Authors: Crocker Dylan B
Published: July 2020

OBJECTIVES/GOALS: Precisely, the goal of the device is to initiate a friction force between the delivery system and the arterial vessel wall to both assure immediate stent deployment and prevent axial advancement of the stent-anchoring wire. METHODS/STUDY POPULATION: A prototype was constructed and its effectiveness of applying a friction force to a vessel wall was tested ex vivo using an LRX Plus Materials Testing Machine. Afterwards, the experimental performance of the device was compared to that of a finite element simulated model. RESULTS/ANTICIPATED RESULTS: The device demonstrated the ability to apply a friction force to the vessel wall to meet its objective. However, experimental values were consistently greater than those gathered from the simulation. Since the force prescribed by the device is minimal, future work includes increasing the force capabilities of the device and defining force requirements. DISCUSSION/SIGNIFICANCE OF IMPACT: Upon further development and testing, this device can be implemented into endovascular neurosurgery to improve occlusion rates of intracranial aneurysms and reduce patient risk during these operations. CONFLICT OF INTEREST DESCRIPTION: I am pursuing intellectual property on this invention. I was careful not to describe the invention in too much detail in my abstract submission for this reason. This research is my thesis work, and I placed on one year embargo on it before it is published to give us time to sort out IP. I would like to be considered for inclusion in Translational Science 2020 if I am able to get IP on this work before publishing, which I expect will be the case. I have every intention of obtaining IP before the conference in April 2020.
4324 Phase 1 Sterile Product Formulation and Manufacturing at Academic Medical Centers: An Introduction for Translational Researchers
Authors: MacArthur Robert Bruce , Rockwell Kenneth , Johnson Amber , Vaughan Roger , Coller Barry S.
Published: July 2020

OBJECTIVES/GOALS: To facilitate the development of innovative injection products by providing translational researchers with a regulatory and manufacturing road map for producing small batch sterile products for Phase 1 research use. To leverage recent AMC investments in facility improvements and pharmacy training in the areas of sterile product production, testing, and environmental controls, that can be used to support production of phase 1 clinical trial supplies METHODS/STUDY POPULATION: Searching and organizing relevant data and information from web portals and databases in the following: areas: FDA, EMA, USP regulations, regulatory science, pharmaceutical formulation and analytics, supply vendors, analytical testing laboratories, and product testing laboratories. Present the information using a user friendly format including flow charts and development timelines, taking the perspective of the translational investigator. RESULTS/ANTICIPATED RESULTS:

Choosing AMC resources vs outside consultants and vendors, leveraging local resources where possible

Qualifying and monitoring suppliers, testing laboratories, in-house departments, and Contract Drug Manufacturing Organizations (CDMO)

Bringing together the deliverables for the IND CMC section

Where and how to leverage available products and science to simplify safe and reliable production

DISCUSSION/SIGNIFICANCE OF IMPACT: Use and utility of injectable drug products, both small molecule and biologics, is growing rapidly, and is projected to continue to escalate well into the next decade. This is due not only to advances in medicine, but also to improvements in AMC-based sterile product production, and a better understanding of small batch manufacturing methods. All three trends align in academic medical centers (AMC) and can be utilized by translational researchers, if they can understand the potential and regulatory requirements.
4328 Translational Fellows as a mechanism to improve throughput of university technology commercialization
Authors: Hall Everett Gordon , Krenning Tom , Seper Michael , Kinch Michael
Published: July 2020

OBJECTIVES/GOALS: The development of early university technologies for commercialization is largely inefficient and exhibits a high rate of failure, often due to a lack of researcher time and commercialization experience. We have created the Translational Fellow role to address these needs and increase the throughput of university technology transfer. METHODS/STUDY POPULATION: Translational Fellows will first build their initial competencies to identify, evaluate, and develop new technologies through internships with intake organizations within the university ecosystem, including the Office of Technology Management, the LEAP gap-funding mechanism, and local venture capital firms. Following this training, Fellows will provide tailored support to validated projects by establishing development milestones, liaising with industry experts, navigating regulatory requirements, and drafting marketing materials such as executive summaries and financial projections. Lastly, Fellows will partner with a highly developed project to facilitate the commercialization of the technology, whether through a SBIR/STTR grant, direct licensing event, or startup creation. RESULTS/ANTICIPATED RESULTS: We anticipate that implementation of this mechanism will increase the proportion of university-generated inventions that undergo successful commercialization events, as well as increase the rate at which these projects develop after initial validation. Furthermore, we expect that the skills acquired through this program will allow Fellows to successfully transition to a variety of roles in the biotech space. We also expect that Fellows will be capable of training other scientific teams in the preparation of SBIR/STTR grants, further expanding opportunities for commercialization in the research space. DISCUSSION/SIGNIFICANCE OF IMPACT: Translational Fellows fill a unique interdisciplinary niche, allowing them to address common barriers faced by academic inventors. Improving commercialization throughput further capitalizes on the wealth of ideas generated in universities, thereby driving innovation in the biomedical space and directly contributing to improved human health. CONFLICT OF INTEREST DESCRIPTION: The authors have no conflicts of interest.
4494 Predictors of Reintubation After Cardiac Surgery
Authors: Freundlich Robert Edward , Li Gen , Wanderer Jonathan P , Billings Frederic T , Domenico Henry , Byrne Daniel , Pandharipande Pratik
Published: July 2020

OBJECTIVES/GOALS: We modeled risk of reintubation within 48 hours of cardiac surgery using variables available in the electronic health record (EHR). This model will guide recruitment for a prospective, pragmatic clinical trial entirely embedded within the EHR among those at high risk of reintubation. METHODS/STUDY POPULATION: All adult patients admitted to the cardiac intensive care unit following cardiac surgery involving thoracotomy or sternotomy were eligible for inclusion. Data were obtained from operational and analytical databases integrated into the Epic EHR, as well as institutional and departmental-derived data warehouses, using structured query language. Variables were screened for inclusion in the model based on clinical relevance, availability in the EHR as structured data, and likelihood of timely documentation during routine clinical care, in the hopes of obtaining a maximally-pragmatic model. RESULTS/ANTICIPATED RESULTS: A total of 2325 patients met inclusion criteria between November 2, 2017 and November 2, 2019. Of these patients, 68.4% were male. Median age was 63.0. The primary outcome of reintubation occurred in 112/2325 (4.8%) of patients within 48 hours and 177/2325 (7.6%) at any point in the subsequent hospital encounter. Univariate screening and iterative model development revealed numerous strong candidate predictors (ANOVA plot, figure 1), resulting in a model with acceptable calibration (calibration plot, figure 2), c = 0.666. DISCUSSION/SIGNIFICANCE OF IMPACT: Reintubation is common after cardiac surgery. Risk factors are available in the EHR. We are integrating this model into the EHR to support real-time risk estimation and to recruit and randomize high-risk patients into a clinical trial comparing post-extubation high flow nasal cannula with usual care. CONFLICT OF INTEREST DESCRIPTION: REF has received grant funding and consulting fees from Medtronic for research on inpatient monitoring.
4470 Are nurses’ attitudes toward caring for hospitalized adults with intellectual disabilities associated with nurse and nursing unit characteristics?
Authors: Desroches Melissa Lynn
Published: July 2020

OBJECTIVES/GOALS: (1) Determine nurse (age, education level, years of experience, ID education/training, contact with people with ID, communication apprehension, beliefs about patient quality of life), and nursing unit (teamwork, staffing and resources, person-centered care) characteristics that are associated with and predictive of nurses’ attitudes, positive emotions, and negative emotions toward caring for adults with ID. (2) Explore nurses’ perspectives of perceived barriers and facilitators to providing nursing care to hospitalized persons with ID and medical comorbidity, and how nursing care differs when caring for a person with ID. METHODS/STUDY POPULATION: This mixed methods nested analysis will employ an internet survey of medical-surgical registered nurses to collect nurses’ attitudes and emotions toward caring for hospitalized persons with ID and medical comorbidity, nurse characteristics, and nursing unit characteristics. We intend to recruit 150 medical surgical nurses currently practicing in the United States via email invitation to the membership of the Academy of Medical Surgical Nurses. Purposeful maximum variation sampling will be used to invite a subset of respondents for qualitative, semi-structured telephone interviews to elicit barriers and facilitators to nursing care of persons with ID and how nursing care differs when caring for persons with ID. RESULTS/ANTICIPATED RESULTS: We hypothesize that lower nurse education level, fewer years of experience, less ID education/training, lower amount of contact with people with ID, increased communication apprehension, and lower beliefs about the quality of life of persons with ID will be associated with negative nurse attitudes and emotions toward caring for people with ID. Further, we hypothesize that lower levels of nursing unit teamwork, staffing and resources, and person-centered care practices will be associated with negative nurse attitudes and emotions toward caring for people with ID. DISCUSSION/SIGNIFICANCE OF IMPACT: The proposed research is an important first step in determining potential nurse and nursing unit factors influencing nurses’ attitudes toward caring for people with ID. It will lead to targeted interventions to enhance nursing care quality and reduce hospital-associated healthcare disparities among people hospitalized with intellectual disabilities and medical comorbidities.
4140 Medical Students Perception of Plastic and Reconstructive Surgery and the Impact of Social Media Influencing their Opinion
Authors: Dayani Fara , Rahgozar Paymon
Published: July 2020

OBJECTIVES/GOALS: The discipline of plastic and reconstructive surgery (PRS) is poorly understood by the public, primary care physicians, and nurses. The aim of our study is to assess medical students’ knowledge and perceptions of PRS as a discipline and explore factors influencing these opinions. METHODS/STUDY POPULATION: To assess medical student’s knowledge and perception of PRS, we distributed an online survey to all medical students at all training levels (i.e. first year to fourth year) enrolled at UCSF School of Medicine, San Francisco, CA during 2019-2020 academic year. In the survey, participants were asked to match 12 surgical subspecialties with 36 operative procedure scenarios. In addition, the survey included questions investigating the most common social medical platform used by medical students and the role of medical social media accounts in contributing to their knowledge of surgical subspecialties. RESULTS/ANTICIPATED RESULTS: Medical students demonstrated a profound gap in knowledge in plastic surgery. The majority of respondents correctly identified plastic surgeons as the primary surgeons performing the cosmetic procedures listed (abdominoplasty, facelift, and liposuction). PRS was identified as the primary specialty involved in breast reconstruction (94.4%) and burns surgery (88.9%). There was poor understanding of the role of plastic surgeons in hand surgery(16.6%), craniofacial surgery(14.8%), and head and neck cancer surgery(9.3%). 52.4% of respondents follow medical social media accounts and 45.6% of respondents indicated that social media contributed to their knowledge of surgical subspecialties. DISCUSSION/SIGNIFICANCE OF IMPACT: Medical students, who form the next generation of doctors, have limited knowledge regarding versatile applications of PRS. Misconceptions about the discipline of PRS negatively impacts resource allocation and hinders the delivery of care to patients that would profoundly benefit from this specialty. CONFLICT OF INTEREST DESCRIPTION: No authors have financial disclosures or conflicts of interest to declare.
4443 Tailoring Professional Development to CTS Trainees
Authors: Maxwell Megan , Hexner Elizabeth , McGarrigle Rachel , Meagher Emma
Published: July 2020

OBJECTIVES/GOALS: Penn instituted a Professional Development Core (PDC) to complement existing CTS education programs. Sessions were designed to advance participant knowledge and skills in key competency areas including communication, expectation setting, implicit bias and organizational structure, self-efficacy and resilience in order to enhance abilities to successfully execute career and research goals. METHODS/STUDY POPULATION: The PDC enrolled 4 cohorts totaling 87 trainees and scholars from 2016-2019. This included 35% predoctoral trainees (27 MD, 3 PhD), 39% postdoctoral trainees (29 MD, 3 PhD, 2 VMD/DVM), and 26% junior faculty (16 MD, 6 MD/PhD, 1 PhD). The two-year curriculum consists of 14 lunchtime sessions held bimonthly during academic terms. Session structures include a variety of interactive presentations, activities and facilitated discussions as well as reading material, assessment tools, and case studies. Facilitators include topic experts in academia, entrepreneurship, communications, and professional and personal development. The program is evaluated qualitatively through student satisfaction surveys after each session. RESULTS/ANTICIPATED RESULTS: Of the 2018-2019 participants, 90.8% rated the overall quality of PDC sessions as Very Good (56.05%) or Outstanding (34.75%). DISCUSSION/SIGNIFICANCE OF IMPACT: Feedback indicates that the group benefited from combining predoctoral and postdoctoral trainees, although not all content was immediately relevant to early stage trainees. Trainees appreciated the opportunity to engage with experts in disciplines typically considered outside of traditional science but critical to CTS career success. The flexibility of the curriculum allowed for inclusion of timely topics, newer suites of sessions focus on the multiple dimensions of valuing your science.
4039 The Development of a Mentored Writing Workgroup
Authors: Capili Bernadette , Capili Bernadette , Walker Jeanne , Coller Barry , Brown Kate
Published: July 2020

OBJECTIVES/GOALS: The purpose of the mentored writing workgroup developed at The CCTS at the Rockefeller University (CCTS RU) was to promote scholarship among clinical research nurses (CRN). The goal was to publish and/or present their projects at national professional association meetings. METHODS/STUDY POPULATION: A two-part writing workshop was presented at RU in December 2018 and January 2019. Members of the RU nursing staff and CRNs from local institutions were invited to attend. Twenty-four CRNs participated in the workshops. The first workshop focused on the writing process, styles of writing and how to get started. The second concentrated on components of a manuscript, categories of papers, selection of the journal, and communications with the editorial team. After the workshops, the CRNs from RU was offered the opportunity to participate in the mentored writing workgroup. To participate, the CRN had to agree to attend scheduled writing meetings and submit written work for each session. The scheduled submission of written materials ensured the CRN was committed to completing their manuscripts or presentations. RESULTS/ANTICIPATED RESULTS: Three CRNs from RU participated in the writing workgroup. Two papers were accepted for publication, and one manuscript is under-review, two abstracts accepted by an international professional organization, and two presentations conducted at an area nursing school and medical center. DISCUSSION/SIGNIFICANCE OF IMPACT: A mentored writing workgroup wherein participants commit to attend writing meetings and to submit written materials in a scheduled-matter can promote scholarship. CONFLICT OF INTEREST DESCRIPTION: NA.
4034 Can Connections IN Health become a research-based model to improve health outcomes through community health coalitions?
Authors: Darbishire Lily , Wiehe Sarah , Savaiano Dennis
Published: July 2020

OBJECTIVES/GOALS: Connections IN Health’s goal is to coordinate, integrate, and enrich health coalition work through extended connections among community and academic stakeholders within and across coalitions and geographies within Indiana. We aim to evaluate stakeholder connections to assess coalition effectiveness and the quality of partnership networks. METHODS/STUDY POPULATION: We will collect data longitudinally to evaluate Connections IN Health using a unique triangulation of effectiveness surveys, social network analysis, and health data. Cross-sectional functioning and social network analysis surveys were distributed to coalition members before the transition to Connections IN Health engagement (baseline) and will be distributed again each year thereafter to identify changes in coalition perceived effectiveness and changes in the structure/nature of partnership networks after implementation of the partnership. We plan to utilize publicly available health data to measure proximal changes in health outcomes at the neighborhood level and use Pearson’s correlations to check for associations between perceived coalition effectiveness and health outcomes. RESULTS/ANTICIPATED RESULTS: We found low baseline scores in perceived effectiveness, especially in the areas of leadership, operational understanding, and satisfaction, from the coalition members. From our social network analysis, we found relatively low cohesion scores (measured as network density) among each of the coalition networks, and even lower scores for collaboration among coalition members. We expect to see positive increases in perceived coalition effectiveness, as well as an increase in the density and level of collaboration among coalition networks as Connections IN Health develops. Finally, we expect to see positive changes in proximal health outcomes associated with our measures of coalition effectiveness. DISCUSSION/SIGNIFICANCE OF IMPACT: The results of our project will be distributed back to the coalition leaders and members in order to sustain and improve the coalitions. The visualization of the coalition member’s network can be used to demonstrate opportunities for enhanced partnerships and collaboration.
4014 Results of a Formative Evaluation of the Cardiopulmonary Vascular Biology (CPVB) Center of Biomedical Research Excellence (COBRE)
Authors: Kimberly Judy , Rounds Sharon , Harrington Elizabeth O. , McNamara Susan
Published: July 2020

OBJECTIVES/GOALS: Results of a formative evaluation of the CardioPulmonary Vascular Biology (CPVB) COBRE will be presented. Of interest were the quality of the overall program, satisfaction with training, mentoring, and services offered, mechanisms for communication, and effectiveness of the collaboration between junior investigators and their mentors. METHODS/STUDY POPULATION: Integral to this evaluation was the creation of questionnaire for junior investigators to complete that addressed four domains: 1) relationship with their mentor, 2) research self-efficacy, 3) administrative and specialty cores value, and 4) satisfaction with events and operations of the COBRE. The two co-principal investigators, program manager, and evaluator developed the 34 items comprising this instrument. The questionnaire was administered online and all eight of the current junior investigators completed the questionnaire. RESULTS/ANTICIPATED RESULTS: Participants were mostly satisfied with the mentoring they were receiving and the operational services of the Administrative and Lab Cores. In terms of training preparedness, these participants felt they were not as prepared as they would like for making adequate progress as an academician and did not feel prepared for managing a lab. Interestingly, these participants felt they were most prepared to develop collaborations with scholars and professionals from other disciplines, but stated they felt they were not as prepared in their abilities to build scientific collaborations. DISCUSSION/SIGNIFICANCE OF IMPACT: Because a primary foci of COBRE grant mechanisms is the development of junior level investigators, evaluating their skills, mentoring experiences, and the usefulness of services is paramount to the sustainability and collaborative research environments of COBREs. This evaluation serves as a model for other COBREs as a tool for measuring this goal.
4127 Achieving health equity in translational research: Applying critical race theory in workforce curricula to address disparity
Authors: Johnson Kristina Gern , Johnston Karen C. , Phillips Jennifer , Gusic Maryellen
Published: July 2020

OBJECTIVES/GOALS: Learners will:

Identify social structures that serve as root causes of health disparities

Critically evaluate the ways in which racism, culture, and power perpetuate disparity

Use critical reflection to shape their research and advocate for institutional change

METHODS/STUDY POPULATION: The Integrated Translational Health Research Institute of Virginia (iTHRIV) Health Equity curriculum provides a lens for participants to view health disparities, social structures that create and perpetuate disparities, and the path to a more equitable future. This longitudinal workforce curriculum incorporates the principles of critical race theory (CRT), including: race as a social construct, structural determinism, intersectionality, and the social construction of knowledge. Learners gain practical experience through facilitated group discussions and critical reflection of their own work including research question design, recruitment, dissemination, and enhancing the faculty pipeline. RESULTS/ANTICIPATED RESULTS: To measure the impact of the curriculum, we will evaluate learners’ participation in mentoring activities for persons from underrepresented backgrounds; participation in local and national diversity and inclusion efforts; engagement in community-based research; ability to account for implicit bias and power imbalances in their research design, including in recruitment and retention; and share research findings with community members and research participants. Evaluation strategies will include quantitative and qualitative methodologies. DISCUSSION/SIGNIFICANCE OF IMPACT: There is growing recognition of the impact of racism on the development and perpetuation of health disparities. Public health critical race praxis (an adaptation of CRT) is emerging as a theoretical framework to empower researchers to challenge the status quo in order to achieve health equity.