bluuebunny/crossref_metadata_2025 huggingface dataset:
de novo Blood Biomarkers in Autism: Autoantibodies against Neuronal and Glial Proteins
Authors: Abou-Donia Mohamed B. , Suliman Hagir B. , Siniscalco Dario , Antonucci Nicola , ElKafrawy Passent , Brahmajothi Mulugu V.
Published: April 2019

Autism spectrum disorders (ASDs) are the most common neurodevelopmental disorders with unidentified etiology. The behavioral manifestations of ASD may be a consequence of genetic and/or environmental pathology in neurodevelopmental processes. In this limited study, we assayed autoantibodies to a panel of vital neuronal and glial proteins in the sera of 40 subjects (10 children with ASD and their mothers along with 10 healthy controls, age-matched children and their mothers). Serum samples were screened using Western Blot analysis to measure immunoglobulin (IgG) reactivity against a panel of 9 neuronal proteins commonly associated with neuronal degeneration: neurofilament triplet proteins (NFP), tubulin, microtubule-associated proteins (tau), microtubule-associated protein-2 (MAP-2), myelin basic protein (MBP), myelin-associated glycoprotein (MAG), α-synuclein (SNCA) and astrocytes proteins such as glial fibrillary acidic protein (GFAP) and S100B protein. Our data show that the levels of circulating IgG class autoantibodies against the nine proteins were significantly elevated in ASD children. Mothers of ASD children exhibited increased levels of autoantibodies against all panel of tested proteins except for S100B and tubulin compared to age-matched healthy control children and their mothers. Control children and their mothers showed low and insignificant levels of autoantibodies to neuronal and glial proteins. These results strongly support the importance of anti-neuronal and glial protein autoantibodies biomarker in screening for ASD children and further confirm the importance of the involvement of the maternal immune system as an index that should be considered in fetal in utero environmental exposures. More studies are needed using larger cohort to verify these results and understand the importance of the presence of such autoantibodies in children with autism and their mothers, both as biomarkers and their role in the mechanism of action of autism and perhaps in its treatment.
A Protective Role of Aryl Hydrocarbon Receptor Repressor in Inflammation and Tumor Growth
Authors: Vogel Christoph F. A. , Ishihara Yasuhiro , Campbell Claire E. , Kado Sarah Y. , Nguyen-Chi Aimy , Sweeney Colleen , Pollet Marius , Haarmann-Stemmann Thomas , Tuscano Joseph M.
Published: April 2019

The aryl hydrocarbon receptor (AhR) is known for mediating the toxicity of environmental pollutants such as dioxins and numerous dioxin-like compounds, and is associated with the promotion of various malignancies, including lymphoma. The aryl hydrocarbon receptor repressor (AhRR), a ligand-independent, transcriptionally inactive AhR-like protein is known to repress AhR signaling through its ability to compete with the AhR for dimerization with the AhR nuclear translocator (ARNT). While AhRR effectively blocks AhR signaling, several aspects of the mechanism of AhRR’s functions are poorly understood, including suppression of inflammatory responses and its putative role as a tumor suppressor. In a transgenic mouse that overexpresses AhRR (AhRR Tg) we discovered that these mice suppress 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)- and inflammation-induced tumor growth after subcutaneous challenge of EL4 lymphoma cells. Using mouse embryonic fibroblasts (MEF) we found that AhRR overexpression suppresses the AhR-mediated anti-apoptotic response. The AhRR-mediated inhibition of apoptotic resistance was associated with a suppressed expression of interleukin (IL)-1β and cyclooxygenase (COX)-2, which was dependent on activation of protein kinase A (PKA) and the CAAT-enhancer-binding protein beta (C/EBPβ). These results provide mechanistic insights into the role of the AhRR to suppress inflammation and highlight the AhRR as a potential therapeutic target to suppress tumor growth.
Bioinspired Polymer-Bound Organocatalysts for Direct Asymmetric Aldol Reaction: Experimental and Computational Studies
Authors: Du Ganhong , Ling Jun , Hu Fangyu , Liu Keyuan , Ye Long , Jiang Liming
Published: April 2019

A series of poly(2-oxazoline) (POX) derivatives bearing prolinamide pendants were designed as organocatalysts and evaluated in the direct asymmetric aldol reaction between aromatic aldehydes and cyclic ketones. The structural variation of the alkyl spacer connecting the polymer backbone with the catalytic unit was applied so as to deduce structure–performance relationships combined with comparable experiments from model catalysts. Results showed that the POX-bound prolinamides can promote the aldol reaction more effectively as compared to their small-molecular and non-POX-bound analogs. The catalyst P3 containing the pyrrolidine moiety closer to the tertiary amide backbone exhibited the overall best catalytic efficiency, affording anti-products in 84% yield with 89% ee in the representative aldol addition of cyclohexanone to 4-nitrobenzaldehyde at a 10 mol.% catalyst loading. Furthermore, the influence of trifluoroacetic acid as an additive on the asymmetric transformation was investigated. Theoretical calculations revealed that the protonation of the aldehyde carbonyl group switched the activation mode of the aldol acceptor through hydrogen bond interactions, thereby changing the relative energy barrier of the enamine/aldehyde reaction transition states, which accounted well for the significant improvement in the enantioselectivity of the acidic additives observed experimentally.
Motor Endplate—Anatomical, Functional, and Molecular Concepts in the Historical Perspective
Authors: Rudolf Rüdiger , Khan Muzamil Majid , Witzemann Veit
Published: April 2019

By mediating voluntary muscle movement, vertebrate neuromuscular junctions (NMJ) play an extraordinarily important role in physiology. While the significance of the nerve-muscle connectivity was already conceived almost 2000 years back, the precise cell and molecular biology of the NMJ have been revealed in a series of fascinating research activities that started around 180 years ago and that continues. In all this time, NMJ research has led to fundamentally new concepts of cell biology, and has triggered groundbreaking advancements in technologies. This review tries to sketch major lines of thought and concepts on NMJ in their historical perspective, in particular with respect to anatomy, function, and molecular components. Furthermore, along these lines, it emphasizes the mutual benefit between science and technology, where one drives the other. Finally, we speculate on potential major future directions for studies on NMJ in these fields.
MAGI1 Mediates eNOS Activation and NO Production in Endothelial Cells in Response to Fluid Shear Stress
Authors: Ghimire Kedar , Zaric Jelena , Alday-Parejo Begoña , Seebach Jochen , Bousquenaud Mélanie , Stalin Jimmy , Bieler Grégory , Schnittler Hans-Joachim , Rüegg Curzio
Published: April 2019

Fluid shear stress stimulates endothelial nitric oxide synthase (eNOS) activation and nitric oxide (NO) production through multiple kinases, including protein kinase A (PKA), AMP-activated protein kinase (AMPK), AKT and Ca2+/calmodulin-dependent protein kinase II (CaMKII). Membrane-associated guanylate kinase (MAGUK) with inverted domain structure-1 (MAGI1) is an adaptor protein that stabilizes epithelial and endothelial cell-cell contacts. The aim of this study was to assess the unknown role of endothelial cell MAGI1 in response to fluid shear stress. We show constitutive expression and co-localization of MAGI1 with vascular endothelial cadherin (VE-cadherin) in endothelial cells at cellular junctions under static and laminar flow conditions. Fluid shear stress increases MAGI1 expression. MAGI1 silencing perturbed flow-dependent responses, specifically, Krüppel-like factor 4 (KLF4) expression, endothelial cell alignment, eNOS phosphorylation and NO production. MAGI1 overexpression had opposite effects and induced phosphorylation of PKA, AMPK, and CAMKII. Pharmacological inhibition of PKA and AMPK prevented MAGI1-mediated eNOS phosphorylation. Consistently, MAGI1 silencing and PKA inhibition suppressed the flow-induced NO production. Endothelial cell-specific transgenic expression of MAGI1 induced PKA and eNOS phosphorylation in vivo and increased NO production ex vivo in isolated endothelial cells. In conclusion, we have identified endothelial cell MAGI1 as a previously unrecognized mediator of fluid shear stress-induced and PKA/AMPK dependent eNOS activation and NO production.
Cholesterol Trafficking: An Emerging Therapeutic Target for Angiogenesis and Cancer
Authors: Lyu Junfang , Yang Eun Ju , Shim Joong Sup
Published: April 2019

Cholesterol is an essential structural component of cellular membranes. In addition to the structural role, it also serves as a precursor to a variety of steroid hormones and has diverse functions in intracellular signal transduction. As one of its functions in cell signaling, recent evidence suggests that cholesterol plays a key role in regulating angiogenesis. This review discusses the role of cholesterol in angiogenesis, with a particular emphasis on cholesterol trafficking in endothelial cell signaling. Small molecule inhibitors of cholesterol trafficking and their preclinical and clinical development targeting angiogenesis and cancer are also discussed.
A Cleared View on Retinal Organoids
Authors: Cora Virginia , Haderspeck Jasmin , Antkowiak Lena , Mattheus Ulrich , Neckel Peter , Mack Andreas , Bolz Sylvia , Ueffing Marius , Pashkovskaia Natalia , Achberger Kevin , Liebau Stefan
Published: April 2019

Human induced pluripotent stem cell (hiPSC)-derived organoids mimicking tissues and organs in vitro have advanced medical research, as they opened up new possibilities for in-depth basic research on human organ development as well as providing a human in vitro model for personalized therapeutic approaches. hiPSC-derived retinal organoids have proven to be of great value for modeling the human retina featuring a very similar cellular composition, layering, and functionality. The technically challenging imaging of three-dimensional structures such as retinal organoids has, however, raised the need for robust whole-organoid imaging techniques. To improve imaging of retinal organoids we optimized a passive clearing technique (PACT), which enables high-resolution visualization of fragile intra-tissue structures. Using cleared retinal organoids, we could greatly enhance the antibody labeling efficiency and depth of imaging at high resolution, thereby improving the three-dimensional microscopy output. In that course, we were able to identify the spatial morphological shape and organization of, e.g., photoreceptor cells and bipolar cell layers. Moreover, we used the synaptic protein CtBP2/Ribeye to visualize the interconnection points of photoreceptor and bipolar cells forming the retinal-specific ribbon synapses.
MapB Protein is the Essential Methionine Aminopeptidase in Mycobacterium tuberculosis
Authors: Vanunu Miriam , Schall Patrick , Reingewertz Tali-Haviv , Chakraborti Pradip K. , Grimm Bernhard , Barkan Daniel
Published: April 2019

M. tuberculosis (Mtb), which causes tuberculosis disease, continues to be a major global health threat. Correct identification of valid drug targets is important for the development of novel therapeutics that would shorten the current 6–9 month treatment regimen and target resistant bacteria. Methionine aminopeptidases (MetAP), which remove the N-terminal methionine from newly synthesized proteins, are essential in all life forms (eukaryotes and prokaryotes). The MetAPs contribute to the cotranslational control of proteins as they determine their half life (N-terminal end rule) and facilitate further modifications such as acetylation and others. Mtb (and M. bovis) possess two MetAP isoforms, MetAP1a and MetAP1c, encoded by the mapA and mapB genes, respectively. Conflicting evidence was reported in the literature on which of the two variants is essential. To resolve this question, we performed a targeted genetic deletion of each of these two genes. We show that a deletion mutant of mapA is viable with only a weak growth defect. In contrast, we provide two lines of genetic evidence that mapB is indispensable. Furthermore, construction of double-deletion mutants as well as the introduction of point mutations into mapB resulting in proteins with partial activity showed partial, but not full, redundancy between mapB and mapA. We propose that it is MetAP1c (mapB) that is essentially required for mycobacteria and discuss potential reasons for its vitality.
Coordination between Rac1 and Rab Proteins: Functional Implications in Health and Disease
Authors: Margiotta Azzurra , Bucci Cecilia
Published: April 2019

The small GTPases of the Rho family regulate many aspects of actin dynamics, but are functionally connected to many other cellular processes. Rac1, a member of this family, besides its known function in the regulation of actin cytoskeleton, plays a key role in the production of reactive oxygen species, in gene transcription, in DNA repair, and also has been proven to have specific roles in neurons. This review focuses on the cooperation between Rac1 and Rab proteins, analyzing how the coordination between these GTPases impact on cells and how alterations of their functions lead to disease.
Thermochemically Stable Liquid-Crystalline Gold(I) Complexes Showing Enhanced Room Temperature Phosphorescence
Authors: Kuroda Yuki , Nakamura Shin-ya , Srinivas Katam , Sathyanarayana Arruri , Prabusankar Ganesan , Hisano Kyohei , Tsutsumi Osamu
Published: April 2019

Gold(I) complexes are some of the most attractive materials for generating aggregation-induced emission (AIE), enabling the realization of novel light-emitting applications such as chemo-sensors, bio-sensors, cell imaging, and organic light-emitting diodes (OLEDs). In this study, we propose a rational design of luminescent gold complexes to achieve both high thermochemical stability and intense room temperature phosphorescence, which are desirable features in practical luminescent applications. Here, a series of gold(I) complexes with ligands of N-heterocyclic carbene (NHC) derivatives and/or acetylide were synthesized. Detailed characterization revealed that the incorporation of NHC ligands could increase the molecular thermochemical stability, as the decomposition temperature was increased to ~300 °C. We demonstrate that incorporation of both NHC and acetylide ligands enables us to generate gold(I) complexes exhibiting both high thermochemical stability and high room-temperature phosphorescence quantum yield (>40%) under ambient conditions. Furthermore, we modified the length of alkoxy chains at ligands, and succeeded in synthesizing a liquid crystalline gold(I) complex while maintaining the relatively high thermochemical stability and quantum yield.
The Photorefractive Response of Zn and Mo Codoped LiNbO3 in the Visible Region
Authors: Xue Liyun , Liu Hongde , Zheng Dahuai , Saeed Shahzad , Wang Xuying , Tian Tian , Zhu Ling , Kong Yongfa , Liu Shiguo , Chen Shaolin , Zhang Ling , Xu Jingjun
Published: April 2019

We mainly investigated the effect of the valence state of photorefractive resistant elements on the photorefractive properties of codoped crystals, taking the Zn and Mo codoped LiNbO3 (LN:Mo,Zn) crystal as an example. Especially, the response time and photorefractive sensitivity of 7.2 mol% Zn and 0.5 mol% Mo codoped with LiNbO3 (LN:Mo,Zn7.2) crystal are 0.65 s and 4.35 cm/J at 442 nm, respectively. The photorefractive properties of the LN:Mo,Zn crystal are similar to the Mg and Mo codoped LiNbO3 crystal, which are better than the Zr and Mo codoped LiNbO3 crystal. The results show that the valence state of photorefractive resistant ions is an important factor for the photorefractive properties of codoped crystals and that the LN:Mo,Zn7.2 crystal is another potential material with fast response to holographic storage.
Optimization of Vapor Diffusion Conditions for Anti-CD20 Crystallization and Scale-Up to Meso Batch
Authors: Yang Huaiyu , Belviso Benny Danilo , Li Xiaoyu , Chen Wenqian , Mastropietro Teresa Fina , Di Profio Gianluca , Caliandro Rocco , Heng Jerry Y. Y.
Published: April 2019

The crystal form is one of the preferred formulations for biotherapeutics, especially thanks to its ability to ensure high stability of the active ingredient. In addition, crystallization allows the recovery of a very pure drug, thus facilitating the manufacturing process. However, in many cases, crystallization is not trivial, and other formulations, such as the concentrate solution, represent the only choice. This is the case of anti-cluster of differentiation 20 (anti-CD20), which is one of the most sold antibodies for therapeutic uses. Here, we propose a set of optimized crystallization conditions for producing anti-CD20 needle-shaped crystals within 24 h in a very reproducible manner with high yield. High crystallization yield was obtained with high reproducibility using both hanging drop vapor diffusion and meso batch, which is a major step forward toward further scaling up the crystallization of anti-CD20. The influence of anti-CD20 storage conditions and the effect of different ions on the crystallization processes were also assessed. The crystal quality and the high yield allowed the first crystallographic investigation on anti-CD20, which positively confirmed the presence of the antibody in the crystals.
What Pattern of Progression in Geoscience Fieldwork can be Recognised by Geoscience Educators?
Authors: King Chris
Published: April 2019

The question in the title was addressed by dividing the elements of geoscience fieldwork into eight different strands and then subdividing these into different items addressed during fieldwork. Separate small groups of experienced geoscience educators (mainly geology teachers of 16–18-year-old and younger students) were then asked to sort the items for each strand into order, from the most simple to the most difficult, and then to assign the items to levels of difficulty identified in the progression of scientific skills given in the National Curriculum for Science in England. The results indicate that, whilst those involved found the exercise fairly difficult, nevertheless it was possible to identify an agreed progression in each of the strands. It is hoped that this exercise will provoke geoscience educators to carry out further research into progression in geoscience fieldwork education, enabling them to confirm such a progression. Meanwhile raising awareness amongst teachers of geoscience fieldwork that there is the opportunity to teach elements of fieldwork in order of increasing difficulty, as identified in the research, should enable them to plan more effective progression in their fieldwork teaching. The strategy and methodology used could also enhance professional development in the teaching of geoscience fieldwork.
Gas Seeps at the Edge of the Gas Hydrate Stability Zone on Brazil’s Continental Margin
Authors: Ketzer Marcelo , Praeg Daniel , Pivel Maria A.G. , Augustin Adolpho H. , Rodrigues Luiz F. , Viana Adriano R. , Cupertino José A.
Published: April 2019

Gas hydrate provinces occur in two sedimentary basins along Brazil’s continental margin: (1) The Rio Grande Cone in the southeast, and (2) the Amazon deep-sea fan in the equatorial region. The occurrence of gas hydrates in these depocenters was first detected geophysically and has recently been proven by seafloor sampling of gas vents, detected as water column acoustic anomalies rising from seafloor depressions (pockmarks) and/or mounds, many associated with seafloor faults formed by the gravitational collapse of both depocenters. The gas vents include typical features of cold seep systems, including shallow sulphate reduction depths (<4 m), authigenic carbonate pavements, and chemosynthetic ecosystems. In both areas, gas sampled in hydrate and in sediments is dominantly formed by biogenic methane. Calculation of the methane hydrate stability zone for water temperatures in the two areas shows that gas vents occur along its feather edge (water depths between 510 and 760 m in the Rio Grande Cone and between 500 and 670 m in the Amazon deep-sea fan), but also in deeper waters within the stability zone. Gas venting along the feather edge of the stability zone could reflect gas hydrate dissociation and release to the oceans, as inferred on other continental margins, or upward fluid flow through the stability zone facilitated by tectonic structures recording the gravitational collapse of both depocenters. The potential quantity of venting gas on the Brazilian margin under different scenarios of natural or anthropogenic change requires further investigation. The studied areas provide natural laboratories where these critical processes can be analyzed and quantified.
Metabolic Syndrome Biomarkers of World Trade Center Airway Hyperreactivity: A 16-Year Prospective Cohort Study
Authors: Kwon Sophia , Crowley George , Mikhail Mena , Lam Rachel , Clementi Emily , Zeig-Owens Rachel , Schwartz Theresa , Liu Mengling , Prezant David , Nolan Anna
Published: April 2019

Airway hyperreactivity (AHR) related to environmental exposure is a significant public health risk worldwide. Similarly, metabolic syndrome (MetSyn), a risk factor for obstructive airway disease (OAD) and systemic inflammation, is a significant contributor to global adverse health. This prospective cohort study followed N = 7486 World Trade Center (WTC)-exposed male firefighters from 11 September 2001 (9/11) until 1 August 2017 and investigated N = 539 with newly developed AHR for clinical biomarkers of MetSyn and compared them to the non-AHR group. Male firefighters with normal lung function and no AHR pre-9/11 who had blood drawn from 9 September 2001–24 July 2002 were assessed. World Trade Center-Airway Hyperreactivity (WTC-AHR) was defined as either a positive bronchodilator response (BDR) or methacholine challenge test (MCT). The electronic medical record (EMR) was queried for their MetSyn characteristics (lipid profile, body mass index (BMI), glucose), and routine clinical biomarkers (such as complete blood counts). We modeled the association of MetSyn characteristics at the first post-9/11 exam with AHR. Those with AHR were significantly more likely to be older, have higher BMIs, have high intensity exposure, and have MetSyn. Smoking history was not associated with WTC-AHR. Those present on the morning of 9/11 had 224% increased risk of developing AHR, and those who arrived in the afternoon of 9/11 had a 75.9% increased risk. Having ≥3 MetSyn parameters increased the risk of WTC-AHR by 65.4%. Co-existing MetSyn and high WTC exposure are predictive of future AHR and suggest that systemic inflammation may be a contributor.
Association of ACTN3 Polymorphism with Body Somatotype and Cardiorespiratory Fitness in Young Healthy Adults
Authors: Potocka Natalia , Penar-Zadarko Beata , Skrzypa Marzena , Braun Marcin , Zadarko-Domaradzka Maria , Ozimek Mariusz , Nizioł-Babiarz Edyta , Barabasz Zbigniew , Zawlik Izabela , Zadarko Emilian
Published: April 2019

ACTN3 encodes the protein α-actinin-3, which affects the muscle phenotype. In the present study, we examined the association of ACTN3 R577X polymorphism with body somatotype and cardiorespiratory fitness in young, healthy adults. The study group included 304 young adults, in whom cardiorespiratory fitness was evaluated and the maximum oxygen uptake was determined directly. The somatotype components were calculated according to the Heath-Carter method. Genotyping for the ACTN3 gene was performed using a polymerase chain reaction followed by high-resolution melting analysis. In the female group, a lower maximal heart rate (HRmax) was more strongly associated with the RR genotype (p = 0.0216) than with the RX and XX genotypes. In the male group, the ACTN3 RX genotype, as compared with other genotypes, tended to be associated with a lower percentage of adipose tissue (p = 0.0683), as also reflected by the body mass index (p = 0.0816). ACTN3 gene polymorphism may affect cardiorespiratory fitness. Our analysis of ACTN3 gene polymorphism does not clearly illustrate the relationships among genotype, body composition, and somatotype in young, healthy adults.
The Response of Net Primary Production to Climate Change: A Case Study in the 400 mm Annual Precipitation Fluctuation Zone in China
Authors: Li Yang , Qin Yaochen
Published: April 2019

The regions in China that intersect the 400 mm annual precipitation line are especially ecologically sensitive and extremely vulnerable to anthropogenic activities. However, in the context of climate change, the response of vegetation Net Primary Production (NPP) in this region has not been scientifically studied in depth. NPP suffers from the comprehensive effect of multiple climatic factors, and how to eliminate the effect of interfering variables in the correlation analysis of NPP and target variables (temperature or precipitation) is the major challenge in the study of NPP influencing factors. The correlation coefficient between NPP and target variable was calculated by ignoring other variables that also had a large impact on NPP. This increased the uncertainty of research results. Therefore, in this study, the second-order partial correlation analysis method was used to analyze the correlation between NPP and target variables by controlling other variables. This can effectively decrease the uncertainty of analysis results. In this paper, the univariate linear regression, coefficient of variation, and Hurst index estimation were used to study the spatial and temporal variations in NPP and analyze whether the NPP seasonal and annual variability will persist into the future. The results show the following: (i) The spatial distribution of NPP correlated with precipitation and had a gradually decreasing trend from southeast to northwest. From 2000 to 2015, the NPP in the study area had a general upward trend, with a small variation in its range. (ii) Areas with negative partial correlation coefficients between NPP and precipitation are consistent with the areas with more abundant water resources. The partial correlation coefficient between the NPP and the Land Surface Temperature (LST) was positive for 52.64% of the total study area. Finally, the prediction of the persistence of NPP variation into the future showed significant differences on varying time scales. On an annual scale, NPP was predicted to persist for 46% of the study area. On a seasonal scale, NPP in autumn was predicted to account for 49.92%, followed by spring (25.67%), summer (13.40%), and winter (6.75%).
Effectiveness Analysis of Systematic Combined Sewer Overflow Control Schemes in the Sponge City Pilot Area of Beijing
Authors: Gong Yongwei , Chen Ye , Yu Lei , Li Junqi , Pan Xingyao , Shen Zhenyao , Xu Xiang , Qiu Qianying
Published: April 2019

Combined sewer overflow (CSO) pollution poses a serious threat to the urban water environment and is more severe in old urban areas. This research uses the old urban area in the sponge city pilot area in Tongzhou District, Beijing, as the study area. The United States Environmental Protection Agency (USEPA) storm water management model (SWMM) was used to establish the hydrologic and hydraulic model of this area. The model parameters were calibrated and validated based on the measured rainfall and runoff data. The results show that the Nash-Sutcliffe efficiency coefficient for calibration and validation is more than 0.74. Thirty-two sets of systematic CSO control schemes are formulated, which include the "gray (includes the pipes, pumps, ditches, and detention ponds engineered by people to manage stormwater) strategy" and "gray-green strategies", and the regularity of CSO control for "low impact development (LID) facilities at the source", "intercepting sewer pipes at the midway", and "storage tank at the end", are quantitatively analyzed. The results show that the LID facility has an average annual reduction rate of 22% for the CSO frequency and 35% to 49% for the CSO volume. The retrofitting of intercepting sewer pipes has an average annual reduction rate of 11% for the CSO frequency and 4% to 15% for the CSO volume, and the storage tank has an average annual reduction rate from 3% to 36% for the CSO volume; furthermore, the reduction rate decreases with the increase in the CSO volume reduction rate by LID facilities. When the CSO control target is stricter, the control effect of the "end" segment is more obvious, but the control efficiency is lower. By studying the variability of the storage tank volume under different control targets, it can be concluded that it is reasonable to set the CSO control target because the number of overflow events does not exceed four times per year for the study area.
The Nature and Cost of Readmissions after Work-Related Traumatic Spinal Injuries in New South Wales, Australia
Authors: Sharwood Lisa N. , Möller Holger , Young Jesse T. , Vaikuntam Bharat , Ivers Rebecca Q. , Driscoll Tim , Middleton James W.
Published: April 2019

This study aimed to measure the subsequent health and health service cost burden of a cohort of workers hospitalised after sustaining work-related traumatic spinal injuries (TSI) across New South Wales, Australia. A record-linkage study (June 2013–June 2016) of hospitalised cases of work-related spinal injury (ICD10-AM code U73.0 or workers compensation) was conducted. Of the 824 individuals injured during this time, 740 had sufficient follow-up data to analyse readmissions ≤90 days post-acute hospital discharge. Individuals with TSI were predominantly male (86.2%), mean age 46.6 years. Around 8% (n = 61) experienced 119 unplanned readmission episodes within 28 days from discharge, over half with the primary diagnosis being for care involving rehabilitation. Other readmissions involved device complications/infections (7.5%), genitourinary or respiratory infections (10%) or mental health needs (4.3%). The mean ± SD readmission cost was $6946 ± $14,532 per patient. Unplanned readmissions shortly post-discharge for TSI indicate unresolved issues within acute-care, or poor support services organisation in discharge planning. This study offers evidence of unmet needs after acute TSI and can assist trauma care-coordinators’ comprehensive assessments of these patients prior to discharge. Improved quantification of the ongoing personal and health service after work-related injury is a vital part of the information needed to improve recovery after major work-related trauma.
Developing A Case-Based Reasoning Model for Safety Accident Pre-Control and Decision Making in the Construction Industry
Authors: Su Yikun , Yang Shijing , Liu Kangning , Hua Kaicheng , Yao Qi
Published: April 2019

Case-based reasoning (CBR) has been extensively employed in various construction management areas, involving construction cost prediction, duration estimation, risk management, tendering, bidding and procurement. However, there has been a dearth of research integrating CBR with construction safety management for preventing safety accidents. This paper proposes a CBR model which focuses on case retrieval and reuse to provide safety solutions for new problems. It begins with the identification of case problem attribute and solution attribute, the state of hazard is used to describe the problem attribute based on principles of people’s unsafe behavior and objective’s unsafe state. Frame-based knowledge representation method is adopted to establish the case database from dimensions of slot, facet and facet’s value. Besides, cloud graph method is introduced to determine the attribute weight through analyzing the numerical characteristics of expectation value, entropy value and hyper entropy value. Next, thesaurus method is employed to calculate the similarity between cases including word level similarity and sentence level similarity. Principles and procedures have been provided on case revise and case retain. Finally, a real-world case is conducted to illustrate the applicability and effectiveness of the proposed model. Considering the high potential for pre-control and decision-making of construction safety accident, the proposed model is expected to contribute safety managers to take decisions on prevention measures more efficiently.
Persistence of Immunity for Hepatitis B Virus among Heathcare Workers and Italian Medical Students 20 Years after Vaccination
Authors: Coppeta Luca , Pompei Andrea , Balbi Ottavia , De Zordo Ludovico M. , Mormone Federica , Policardo Sara , Lieto Piergiorgio , Pietroiusti Antonio , Magrini Andrea
Published: April 2019

Background: Immunization of healthcare workers (HCWs) and medical students for the hepatitis B virus (HBV) is a crucial part of the hospital infection control programs. The aim of our study was to evaluate the persistence of anti-HBV specific antibodies in HCWs vaccinated during infancy or adolescence. Methods: Medical records of 734 consecutive subjects born after 1980 (481 females, 65.5% and 253 males, 34.5%) who underwent serological testing for anti-hepatitis B surface antibodies (anti-HBs) were evaluated. Results: A non-protective titer (<10 mUI) was found in 88/734 (12.0%) subjects; 84 (47.8%) of them received a booster dose of anti-hepatitis B vaccine and the anti-HBs titer of 58 subjects was measured 1 month after administration. A protective titer (anti-HBs >10 mIU/mL) was observed in almost 90% of subjects receiving the booster dose. Conclusions: A substantial percentage of HCWs had a non-protective anti-HBs titer at the time of the first employment, especially those vaccinated at birth age. However, the response to the booster dose showed that in these subjects, an anti-HBs titer <10 mIU/mL was due to the physiological decline of antibodies over the years. Therefore, primary immunization in childhood is highly effective and provides lasting immunity against HBV infection.
Reducing Inequities in Early Childhood Mental Health: How Might the Neighborhood Built Environment Help Close the Gap? A Systematic Search and Critical Review
Authors: Alderton Amanda , Villanueva Karen , O’Connor Meredith , Boulangé Claire , Badland Hannah
Published: April 2019

Background: Optimal mental health in early childhood is key to later mental health, physical health, education, and social outcomes; yet, children facing disadvantage tend to have worse mental health and fewer opportunities to develop this foundation. An emerging body of research shows that neighborhoods provide important opportunities for the development of children’s mental health. Synthesizing this evidence can advance understandings of the features of the neighborhood built environment (e.g., housing, parks) that (1) promote optimal mental health in childhood and (2) reduce mental health inequities. Methods: We systematically searched and critically reviewed the international quantitative literature investigating associations between the neighborhood built environment and young children’s mental health. Results: 14 articles met inclusion criteria; most examined nature or public open space. Studies tended to find greater access to or quantity of neighborhood nature or public open space were associated with better mental health. Significant gaps included a lack of studies investigating social infrastructure, and few studies examined how the built environment related to positive mental health (i.e., functioning, rather than problems). Conclusions: Current evidence suggests there is some relationship, but additional research is needed that addresses these gaps and examines differences in associations between child subgroups (e.g., diverse socioeconomic backgrounds).
The Effect of Innovation-Driven Strategy on Green Economic Development in China—An Empirical Study of Smart Cities
Authors: Cao Wenbin , Zhang Ying , Qian Peng
Published: April 2019

Of all the countries presently industrializing, both going green and going smart ideas can be likened to green development, in that they are helping to address or identify new routes out of environment and development conflicts. Environmental pollution issues loom large for China, and the scale of its transformation makes its proposed solutions of widespread interest. China has begun to decouple some environmental pressure from economic growth, and an innovation-driven strategy is proposed to deal with the synergic development between the economy and environment. Considering both positive and negative effects of the innovation-driven strategy on green economic development, the aim of this paper is to find out the influence path and mechanism of policies and measures of the innovation strategy on green economic development, through the empirical analysis of panel data of 47 pilot smart cities in China from 2009 to 2017. The results show that the innovation-driven strategy has a positive effect on green development, while different innovation-driven indicators play different roles in the promotion of economic green development. Practically, this research provides a decision-making reference for sustainable development policy formulation in developing countries, especially in emerging economies.
Termites (Blattodea Latreille 1810, Termitoidae Latreille 1802) of Abuko Nature Reserve, Nyambai Forest Park and Tanji Bird Reserve (The Gambia)
Authors: Ndiaye Abdoulaye Baila , Njie Ebrima , Correa Paul A.
Published: April 2019

From 28 October to 5 November 2013, a termite study was undertaken in 3 protected sites in The Gambia (West Africa). The aim of the study is to investigate the diversity of termites in three protected areas in the western region of the country. Termite sampling is carried out in 100 m × 2 m transects that are replicated three (3) times in each site. A total of thirty-one (31) termite species, that belong to fungus growing (11), harvester (1), humuvorous (12) and xylophagous (7), were recorded. The following nineteen (19) species are new to The Gambia: Coptotermes intermedius, Astalotermes near quietus, Ancistrotermes cavithorax, Macrotermes bellicosus, Microtermes grassei, M. lepidus, M. subhyalinus, Odontotermes erraticus, O. pauperans, O. sudanensis, Basidentitermes sp., Euchilotermes tensus arcuata, Noditermes cristifrons, Amitermes evuncifer, Amitermes spinifer, Microcerotermes fuscotibialis, Microcerotermes near parvulus, Microcerotermes near solidus and Promirotermes holmgreni. Additional description and/or ecological information on Odontotermes erraticus, Cubitermes severus, Cubitermes n. proximatus, Euchilotermes tensus arcuata, Basidentitermes sp., and Noditermes cristifrons are given.
Cardiovascular Risks Associated with Gender and Aging
Authors: Rodgers Jennifer L. , Jones Jarrod , Bolleddu Samuel I. , Vanthenapalli Sahit , Rodgers Lydia E. , Shah Kinjal , Karia Krishna , Panguluri Siva K.
Published: April 2019

The aging and elderly population are particularly susceptible to cardiovascular disease. Age is an independent risk factor for cardiovascular disease (CVD) in adults, but these risks are compounded by additional factors, including frailty, obesity, and diabetes. These factors are known to complicate and enhance cardiac risk factors that are associated with the onset of advanced age. Sex is another potential risk factor in aging adults, given that older females are reported to be at a greater risk for CVD than age-matched men. However, in both men and women, the risks associated with CVD increase with age, and these correspond to an overall decline in sex hormones, primarily of estrogen and testosterone. Despite this, hormone replacement therapies are largely shown to not improve outcomes in older patients and may also increase the risks of cardiac events in older adults. This review discusses current findings regarding the impacts of age and gender on heart disease.
The Transcriptomic Toolbox: Resources for Interpreting Large Gene Expression Data within a Precision Medicine Context for Metabolic Disease Atherosclerosis
Authors: Marín de Evsikova Caralina , Raplee Isaac D. , Lockhart John , Jaimes Gilberto , Evsikov Alexei V.
Published: April 2019

As one of the most widespread metabolic diseases, atherosclerosis affects nearly everyone as they age; arteries gradually narrow from plaque accumulation over time reducing oxygenated blood flow to central and periphery causing heart disease, stroke, kidney problems, and even pulmonary disease. Personalized medicine promises to bring treatments based on individual genome sequencing that precisely target the molecular pathways underlying atherosclerosis and its symptoms, but to date only a few genotypes have been identified. A promising alternative to this genetic approach is the identification of pathways altered in atherosclerosis by transcriptome analysis of atherosclerotic tissues to target specific aspects of disease. Transcriptomics is a potentially useful tool for both diagnostics and discovery science, exposing novel cellular and molecular mechanisms in clinical and translational models, and depending on experimental design to identify and test novel therapeutics. The cost and time required for transcriptome analysis has been greatly reduced by the development of next generation sequencing. The goal of this resource article is to provide background and a guide to appropriate technologies and downstream analyses in transcriptomics experiments generating ever-increasing amounts of gene expression data.
Simulation of the Grondona System of Conditional Currency Convertibility Based on Primary Commodities, Considered as a Means to Resist Currency Crises
Authors: Collins Patrick , Ahmed Jameel , Meera Ahamed Kameel
Published: April 2019

Currency crises are a significant feature of the present-day world economy, in which financial transactions are many times larger than monetary flows in the “real economy”, so that defending a currency’s exchange-rate is a major challenge for the governments of countries which may be smaller than a single large corporation. It is made even more difficult due to the United States government and its agents openly using economic pressures to try to force other countries to obey its orders, even including regime change. Guaranteed convertibility of a currency, such as maintaining a gold standard, can in principle help to stabilise its value, but this has been absent since the end of US dollar convertibility in 1971. The Grondona system of conditional currency convertibility was not planned as a counter-measure for currency crises. However the simulation of its operation demonstrated in this paper shows clearly how its automatic counter-cyclical stock-holding in response to movements in commodity prices—and so to exchange-rate movements that alter domestic commodity prices—causes monetary flows that would resist large exchange-rate movements (among other effects), and thereby tend to ameliorate a currency crisis. Moreover, it would achieve this without the need for international negotiations, agreements or other geopolitical trade-offs.
Burn Time of Metal Nanoparticles
Authors: Altman Igor
Published: April 2019

This article will discuss the combustion of metal nanoparticles and explain the burn time dependence on particle size. In contrary to common belief in the power law (tb~d0.3), which, in our knowledge, is simply an experimental fit to data, we propose the logarithmic law (tb~ln(d)) that describes well the known results on nano-aluminum combustion. We derived the logarithmic dependence from a simple model taking into account the energy balance on the surface of a burning metal nanoparticle. The model in question is based on the small energy accommodation coefficient (EAC), which was recently utilized to solve experimental puzzles such as the significant temperature gap between the burning nanoparticle and the environment. A discussion on EAC, which value is important for the correct modeling of nanoparticle combustion, is also included. A way to generalize the considered combustion model is suggested.
The Effect of Humidity on Dielectric Properties of PP-Based Nano-Dielectric
Authors: Chi Xiaohong , Liu Wenfeng , Li Shengtao , Zhang Xiaohong
Published: April 2019

Nano-dielectrics are sensitive to humidity and easily degraded in damp environment because of the high surface energy of nanoparticles. In order to study the effect of humidity on the dielectric properties of nano-dielectric, polypropylene (PP) was modified by polyolefin elastomer (POE) and nano-SiO2, and the samples with obvious filling concentration were pre-selected by breakdown strength for damp aging. The aging experiments were carried out in different relative humidity. The dielectric properties of new, hygroscopic saturation and samples after drying were measured and analyzed. It is found that the breakdown strength of hygroscopic saturation nano-dielectrics decreased obviously compared with new samples, and it was difficult to recover after drying. The damp degradation resulted in different changing trends of permittivity of PP and nano-dielectric, but there were relaxation loss peaks of water in both of them. The influence of damp degradation on the trap distribution was studied by thermally stimulated depolarization currents (TSDC), and it was found that the traps level introduced by water molecules was different in PP and nano-dielectrics. All experiment results showed that the performance of nano-dielectrics degraded obviously in humid environment, and it was difficult to recover even after complete drying because of the existence of bounded water molecules in nano-dielectrics.
Nano-Montmorillonite Regulated Crystallization of Hierarchical Strontium Carbonate in a Microbial Mineralization System
Authors: Zheng Kui , Chen Tao , Zhang Jian , Tian Xiuquan , Ge Huilin , Qiao Tiantao , Lei Jia , Li Xianyan , Duan Tao , Zhu Wenkun
Published: April 2019

In this paper, nano-montmorillonite (nano-MMT) was introduced into the microbial mineralization system of strontium carbonate (SrCO3). By changing the nano-MMT concentration and the mineralization time, the mechanism of mineralization was studied. SrCO3 superstructures with complex forms were acquired in the presence of nano-MMT as a crystal growth regulator. At low concentrations of nano-MMT, a cross-shaped SrCO3 superstructure was obtained. As the concentration increased, flower-like SrCO3 crystals formed via the dissolution and recrystallization processes. An emerging self-assembly process and crystal polymerization mechanism have been proposed by forming complex flower-like SrCO3 superstructures in high concentrations of nano-MMT. The above research indicated that unique bionic synthesis strategies in microbial systems could not only provide a useful route for the production of inorganic or inorganic/organic composites with a novel morphology and unique structure but also provide new ideas for the treatment of radionuclides.
A Comparison between Anodizing and EBSD Techniques for Primary Particle Size Measurement
Authors: Nafisi Shahrooz , Roccisano Anthony , Ghomashchi Reza , Vander Voort George
Published: April 2019

Proper understanding and knowledge of primary particle or grain size is of paramount importance in manufacturing processes as it directly affects various properties including mechanical behavior. Application of optical microscopy coupled with etching techniques has been used conventionally and in conjunction with color metallography (polarized microscopy) has been the preferred method for grain size measurement. An advanced technique as an alternative to light microscopy is using electron backscatter diffraction (EBSD). A comparison is made between these two techniques using Al-7Si alloy produced with various casting techniques to highlight the cost and time of the sample preparation and analysis for both techniques. Results showed that color metallography is certainly a faster technique with great accuracy and a much cheaper alternative in comparison with EBSD.