bluuebunny/crossref_metadata_2025 huggingface dataset:
Enhanced Frequency-Guided Method with Particle Swarm Optimization for the Phase Recovery of a Single Interferogram
Authors: Quintanar-Sotelo Christian O. , Casillas-Rodriguez Francisco J. , Muñoz-Maciel Jesus , Peña-Lecona Francisco G. , Mora-Gonzalez Miguel
Published: November 2023

Frequency-guided sequential demodulation (FSD) is a technique used to recover the phase of closed fringe patterns when only a single interferogram is available. FSD is a sequential method that follows simple and intuitive processing steps for interferogram demodulation, guided by the frequency of the fringes. The objective of this work is to apply an optimizer block that feeds from the first stages of the FSD and make feedback in such a manner as to optimize the search for guided frequencies. This approach significantly reduces demodulation errors, especially when dealing with high-frequency fringe patterns. The results of using the FSD with and without optimizers are compared. The optimizers used are the Harmonic Search Optimization (HSO) as well as the Particle Swarm Optimization (PSO). Results show that FSD with PSO delivers better results compared with other methods here analyzed.
Evaluation of IPCC Models’ Performance in Simulating Late-Twentieth-Century Weather Patterns and Extreme Precipitation in Southeastern China
Authors: Wang Yongdi , Sun Xinyu
Published: November 2023

Climate model evaluation work has made progress both in theory and practice, providing strong support for better understanding and predicting climate change. However, at the weather scale, there is relatively little assessment of climate models in terms of daily-scale climate phenomena, such as storm frequency and intensity. These weather-scale variables are of significant importance for our understanding of the impacts of climate change. In order to assess the capability of climate models to simulate weather-scale climate patterns, this study employs Self-Organizing Maps (SOMs) for weather pattern classification. By combining different evaluation metrics, varying the number of SOM types, changing the size of the study area, and altering the reference datasets, the climate models are evaluated to ensure the robustness of the assessment results. The results demonstrate that the size of the study area is positively correlated with observed differences, and there are correlations among different evaluation metrics. The highest correlation is observed between evaluation metrics in large-scale and small-scale spatial domains, while the correlation with SOM size is relatively low. This suggests that the choice of evaluation metrics has a minor impact on model ranking. Furthermore, when comparing the correlation coefficients calculated using the same evaluation metrics for different-sized regions, a significant positive correlation is observed. This indicates that variations in the size of the study area do not significantly affect model ranking. Further investigation of the relationship between model performance and different SOM sizes shows a significant positive correlation. The impact of dataset selection on model ranking is also compared, revealing high consistency. This enhances the reliability of model ranking. Taking into account the influence of evaluation metric selection, SOM size, and reanalysis data selection on model performance assessment, significant variations in model ranking are observed. Based on cumulative ranking, the top five models identified are ACCESS1-0, GISS-E2-R, GFDL-CM3, MIROC4h, and GFDL-ESM2M. In conclusion, factors such as evaluation metric selection, study area size, and SOM size should be considered when assessing model ranking. Weather pattern classification plays a crucial role in climate model evaluation, as it helps us better understand model performance in different weather systems, assess their ability to simulate extreme weather events, and improve the design and evaluation methods of model ensemble predictions. These findings are of great significance for optimizing and strengthening climate model evaluation methods and provide valuable insights for future research.
NF-kB Regulation and the Chaperone System Mediate Restorative Effects of the Probiotic Lactobacillus fermentum LF31 in the Small Intestine and Cerebellum of Mice with Ethanol-Induced Damage
Authors: Paladino Letizia , Rappa Francesca , Barone Rosario , Macaluso Filippo , Zummo Francesco Paolo , David Sabrina , Szychlinska Marta Anna , Bucchieri Fabio , Conway de Macario Everly , Macario Alberto J. L. , Cappello Francesco , Marino Gammazza Antonella
Published: November 2023

Probiotics are live microorganisms that yield health benefits when consumed, generally by improving or restoring the intestinal flora (microbiota) as part of the muco-microbiotic layer of the bowel. In this work, mice were fed with ethanol alone or in combination with the probiotic Lactobacillus fermentum (L. fermentum) for 12 weeks. The modulation of the NF-κB signaling pathway with the induction of Hsp60, Hsp90, and IkB-α by the probiotic occurred in the jejunum. L. fermentum inhibited IL-6 expression and downregulated TNF-α transcription. NF-κB inactivation concurred with the restoration of the intestinal barrier, which had been damaged by ethanol, via the production of tight junction proteins, ameliorating the ethanol-induced intestinal permeability. The beneficial effect of the probiotic on the intestine was repeated for the cerebellum, in which downregulation of glial inflammation-related markers was observed in the probiotic-fed mice. The data show that L. fermentum exerted anti-inflammatory and cytoprotective effects in both the small intestine and the cerebellum, by suppressing ethanol-induced increased intestinal permeability and curbing neuroinflammation. The results also suggest that L. fermentum could be advantageous, along with the other available means, for treating intestinal diseases caused by stressors associated with inflammation and dysbiosis.
Developmental Auditory and Speech–Language Performance in Pediatric Cochlear Implantation Recipients with Stable White Matter Lesions
Authors: Fan Huiru , Li Dan , Xie Wen , Wang Jing , Cheng Huamao , Kong Weijia
Published: November 2023

To analyze the association between stable asymptomatic white matter lesions (WMLs) and the cochlear implantation (CI) effect in congenitally deaf children, 43 CI children with stable asymptomatic WMLs determined via preoperative assessments and 86 peers with normal white matter were included. Outcome measurements included closed-set Mandarin Chinese (tone, disyllable, and sentence) recognition tests; categories of auditory performance (CAPs); and speech intelligibility rating (SIR) scales at 1, 12, and 24 months post-CI. Generalized estimating equation (GEE) models were used to analyze the association between WML and outcomes. In the WML group (control group), median CAP and SIR scores were 5 (5) and 4 (4) with mean rates of tone, disyllable, and sentence recognition of 84.8% (89.0%), 87.9% (89.7%), and 85.8% (88.0%) at 24 months post-CI, respectively. Auditory and speech performance improved significantly with implant use. Compared to their peers in the control group, for the participants with stable asymptomatic WMLs, auditory and speech abilities were not significantly different (p > 0.05). Stable asymptomatic WMLs might not be associated with poor auditory and speech intelligibility post-CI, which indicates that it is feasible to use comprehensive assessments to screen suitable candidates with WMLs who are likely to present with a good prognosis.
CT-Guided vs. Navigational Bronchoscopic Biopsies for Solitary Pulmonary Nodules: A Single-Institution Retrospective Comparison
Authors: Chaudry Fawad Aleem , Thivierge-Southidara Maureen , Molina Juan Carlos , Farooqui Samid M. , Hussain Syed Talal , Libermen Moishe
Published: November 2023

Objective: Lung cancer is the second most common cause of death by cancer. Multiple modalities can be used to obtain a tissue sample from a pulmonary nodule. We aimed to compare the yield and adverse events related to transthoracic needle aspiration (TTNA) and Electromagnetic Navigation Biopsy (ENB) at our institution. Methods: This was a single-center retrospective study in which all patients referred for evaluation of a pulmonary lesion over 5 years (1 January 2013 to 31 December 2018) were identified. Our primary outcome was to compare the accuracy of TTNA to that of ENB in establishing the diagnosis of pulmonary lesions. Secondary outcomes included the evaluation of the adverse events and the sensitivity, specificity, positive, and negative predictive value of each modality. Results: A total of 1006 patients were analyzed. The mean age of patients in the TTNA and the ENB group was 67.2 ± 11.2 years and 68.3 ± 9.2 years respectively. Local anesthesia was predominantly used for TTNA and moderate sedation was more commonly used in the ENB group. We found ENB to have an accuracy of 57.1%, with a sensitivity of 40.0%, a specificity of 100.0%, a positive predictive value of 100.0%, and a negative predictive value of 40.0%. As for the TTNA, the accuracy was 75.9%, with a sensitivity of 77.5%, a specificity of 61.5%, a positive predictive value of 95.0%, and a negative predictive value of 22.5%. The rate of clinically significant complications was higher in the TTNA group (8.2%) as compared to the ENB group (4.7%) with a p-value < 0.001. Conclusion: TTNA was superior to ENB-guided biopsy for the diagnostic evaluation of lung nodules. However, the complication rate was much higher in the TTNA group as compared to the ENB group.
Targeting Translation and the Cell Cycle Inversely Affects CTC Metabolism but Not Metastasis
Authors: Bowley Tetiana Y. , Merkley Seth D. , Lagutina Irina V. , Ortiz Mireya C. , Lee Margaret , Tawfik Bernard , Marchetti Dario
Published: November 2023

Melanoma brain metastasis (MBM) is significantly associated with poor prognosis and is diagnosed in 80% of patients at autopsy. Circulating tumor cells (CTCs) are “seeds” of metastasis and the smallest functional units of cancer. Our multilevel approach has previously identified a CTC RPL/RPS gene signature directly linked to MBM onset. We hypothesized that targeting ribogenesis prevents MBM/metastasis in CTC-derived xenografts. We treated parallel cohorts of MBM mice with FDA-approved protein translation inhibitor omacetaxine with or without CDK4/CDK6 inhibitor palbociclib, and monitored metastatic development and cell proliferation. Necropsies and IVIS imaging showed decreased MBM/extracranial metastasis in drug-treated mice, and RNA-Seq on mouse-blood-derived CTCs revealed downregulation of four RPL/RPS genes. However, mitochondrial stress tests and RT-qPCR showed that omacetaxine and palbociclib inversely affected glycolytic metabolism, demonstrating that dual targeting of cell translation/proliferation is critical to suppress plasticity in metastasis-competent CTCs. Equally relevant, we provide the first-ever functional metabolic characterization of patient-derived circulating neoplastic cells/CTCs.
Optical Absorption, Photocarrier Recombination Dynamics and Terahertz Dielectric Properties of Electron-Irradiated GaSe Crystals
Authors: Bereznaya Svetlana A. , Redkin Ruslan A. , Brudnyi Valentin N. , Sarkisov Yury S. , Su Xinyang , Sarkisov Sergey Yu.
Published: November 2023

Optical absorption spectra of 9 MeV electron-irradiated GaSe crystals were studied. Two absorption bands with the low-photon-energy threshold at 1.35 and 1.73 eV (T = 300 K) appeared in the transparency region of GaSe after the high-energy-electron irradiation. The observed absorption bands were attributed to the defect states induced by Ga vacancies in two charge states, having the energy positions at 0.23 and 0.61 eV above the valence band maximum at T = 300 K. The optical pump-terahertz probe technique (OPTP) was employed to study the dark and photoexcited terahertz conductivity and charge carrier recombination dynamics at two-photon excitation of as-grown and 9 MeV electron-irradiated GaSe crystals. The measured values of the differential terahertz transmission at a specified photoexcitation condition were used to extract the terahertz charge carrier mobilities. The determined terahertz charge carrier mobility values were ~46 cm2/V·s and ~14 cm2/V·s for as-grown and heavily electron-irradiated GaSe crystals, respectively. These are quite close to the values determined from the Lorentz–Drude–Smith fitting of the measured dielectric constant spectra. The photo-injection-level-dependent charge carrier lifetimes were determined from the measured OPTP data, bearing in mind the model injection-level dependencies of the recombination rates governed by interband and trap-assisted Auger recombination, bulk and surface Shockley–Read–Hall (SRH) recombination and interband radiative transitions in the limit of a high injection level. It was found that GaSe possesses a long charge carrier lifetime (a~1.9 × 10−6 ps−1, b~2.7 × 10−21 cm3ps−1 and c~1.3 × 10−37 cm6ps−1), i.e., τ~0.53 μs in the limit of a relatively low injection, when the contribution from SRH recombination is dominant. The electron irradiation of as-grown GaSe crystals reduced the charge carrier lifetime at a high injection level due to Auger recombination through radiation-induced defects. It was found that the terahertz spectra of the dielectric constants of as-grown and electron-irradiated GaSe crystals can be fitted with acceptable accuracy using the Lorentz model with the Drude–Smith term accounting for the free-carrier conductivity.
Albuminuria Is Affected by Urinary Tract Infection: A Comparison between Biochemical Quantitative Method and Automatic Urine Chemistry Analyzer UC-3500
Authors: Chou Yi-Ju , Yang Chun-Chun , Chang Shang-Jen , Yang Stephen Shei-Dei
Published: November 2023

The automated urine reagent strip test is a cost-effective tool for detecting albuminuria in patients. However, prior research has not investigated how urinary tract infections (UTIs) affect the test’s accuracy. Therefore, this study aims to assess the impact of UTIs on albuminuria diagnosis using both the biochemical quantitative method and the test strip method of the Fully Automatic Urine Chemistry Analyzer, UC-3500 (Sysmex, Kobe, Japan). From March to December 2019, we prospectively collected midstream urine from adult female UTI patients before and after one week of cephalexin treatment. The urine samples were subjected to culture, routine urinalysis, and albuminuria diagnosis using the biochemical quantitative method and UC-3500. Albuminuria was defined as a urine albumin to creatinine ratio (UACR) ≥ 30 mg/g in the biochemical quantitative method. The results were compared between the two methods. Among fifty-four female patients (average age: 50.5 ± 4.4 years) with UTIs, 24 (44.44%) had transient albuminuria. The quantitative UACR significantly decreased after one week of antibiotic treatment (median: 53 mg/g to 9 mg/g; median difference: −0.54, p < 0.0001). UC-3500 exhibited a higher false positive rate for diagnosing albuminuria during UTIs (42%) compared to after treatment (19%). Its agreement with the biochemical quantitative method was moderate during UTI (κ = 0.49, 95% confidence interval [CI]: 0.24–0.73) and good after treatment (κ = 0.65, 95% CI: 0.45–0.86). UC-3500’s accuracy in diagnosing albuminuria is influenced by UTIs, leading to either transient albuminuria or a false positive reaction of the test strip. UTI should be excluded or treated before its application in albuminuria screening.
Point-of-Care Testing to Differentiate Various Acid–Base Disorders in Chronic Kidney Disease
Authors: Gołębiowski Tomasz , Zmonarski Sławomir , Rożek Wiktoria , Powązka Mateusz , Jerzak Patryk , Gołębiowski Maciej , Kusztal Mariusz , Olczyk Piotr , Stojanowski Jakub , Letachowicz Krzysztof , Banasik Mirosław , Konieczny Andrzej , Krajewska Magdalena
Published: November 2023

Background: Normal-anion-gap metabolic acidosis (AGMA) and high-anion-gap metabolic acidosis (HAGMA) are two forms of metabolic acidosis, which is a common complication in patients with chronic kidney disease (CKD). The aim of this study is to identify the prevalence of various acid–base disorders in patients with advanced CKD using point-of-care testing (POCT) and to determine the relationship between POCT parameters. Methods: In a group of 116 patients with CKD in stages G4 and G5, with a mean age of 62.5 ± 17 years, a sample of arterial blood was taken during the arteriovenous fistula procedure for POCT, which enables an assessment of the most important parameters of acid–base balance, including: pH, base excess (BE), bicarbonate (HCO3−), chloride(Cl−), anion gap (AG), creatinine and urea concentration. Based on this test, patients were categorized according to the type of acidosis-base disorder. Results: Decompensate acidosis with a pH < 7.35 was found in 68 (59%) patients. Metabolic acidosis (MA), defined as the concentration of HCO3− ≤ 22 mmol/L, was found in 92 (79%) patients. In this group, significantly lower pH, BE, HCO3− and Cl− concentrations were found. In group of MA patients, AGMA and HAGMA was observed in 48 (52%) and 44 (48%) of patients, respectively. The mean creatinine was significantly lower in the AGMA group compared to the HAGMA group (4.91 vs. 5.87 mg/dL, p < 0.05). The AG correlated positively with creatinine (r = 0.44, p < 0.01) and urea (r = 0.53, p < 0.01), but there was no correlation between HCO3− and both creatinine (r = −0.015, p > 0.05) and urea (r = −0.07, p > 0.05). The Cl− concentrations correlated negatively with HCO3− (r = −0.8, p < 0.01). Conclusions: The most common type of acid–base disturbance in CKD patients in stages 4 and 5 is AGMA, which is observed in patients with better kidney function and is associated with compensatory hyperchloremia. The initiation of renal replacement therapy was significantly earlier for patients diagnosed with HAGMA compared to those diagnosed with AGMA. The more advanced the CKD, the higher the AG.
Unmasking Pandemic Echoes: An In-Depth Review of Long COVID’s Unabated Cardiovascular Consequences beyond 2020
Authors: Luchian Maria-Luiza , Higny Julien , Benoit Martin , Robaye Benoit , Berners Yannick , Henry Jean-Philippe , Colle Benjamin , Xhaët Olivier , Blommaert Dominique , Droogmans Steven , Motoc Andreea Iulia , Cosyns Bernard , Gabriel Laurence , Guedes Antoine , Demeure Fabian
Published: November 2023

At the beginning of 2020, coronavirus disease 2019 (COVID-19) emerged as a new pandemic, leading to a worldwide health crisis and overwhelming healthcare systems due to high numbers of hospital admissions, insufficient resources, and a lack of standardized therapeutic protocols. Multiple genetic variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been detected since its first public declaration in 2020, some of them being considered variants of concern (VOCs) corresponding to several pandemic waves. Nevertheless, a growing number of COVID-19 patients are continuously discharged from hospitals, remaining symptomatic even months after their first episode of COVID-19 infection. Long COVID-19 or ‘post-acute COVID-19 syndrome’ emerged as the new pandemic, being characterized by a high variability of clinical manifestations ranging from cardiorespiratory and neurological symptoms such as chest pain, exertional dyspnoea or cognitive disturbance to psychological disturbances, e.g., depression, anxiety or sleep disturbance with a crucial impact on patients’ quality of life. Moreover, Long COVID is viewed as a new cardiovascular risk factor capable of modifying the trajectory of current and future cardiovascular diseases, altering the patients’ prognosis. Therefore, in this review we address the current definitions of Long COVID and its pathophysiology, with a focus on cardiovascular manifestations. Furthermore, we aim to review the mechanisms of acute and chronic cardiac injury and the variety of cardiovascular sequelae observed in recovered COVID-19 patients, in addition to the potential role of Long COVID clinics in the medical management of this new condition. We will further address the role of future research for a better understanding of the actual impact of Long COVID and future therapeutic directions.
Oral Complications Associated with the Piercing of Oral and Perioral Tissues and the Corresponding Degree of Awareness among Public and Professionals: A Systematic Review
Authors: Mosaddad Seyed , Talebi Sahar , Hemmat Maryam , Karimi Mohammadreza , Jahangirnia Alireza , Alam Mostafa , Abbasi Kamyar , Yazadaniyan Mohsn , Hussain Ahmed , Tebyaniyan Hamid , Abdollahi Namanloo Reza
Published: November 2023

This study systematically reviews the literature to evaluate the potential relationships between oral/perioral piercing and consequent oral complications in the corresponding society. The second objective was determining public/professional sectors’ awareness of the subject. This research followed PRISMA and Cochrane guidelines for conducting systematic reviews and searching scientific databases, including PubMed, Scopus, Cochrane, and Google Scholar, until April 2023. Cross-sectional, cohort, and case–control studies in English were deemed eligible. The methodological quality of the included studies was assessed using proper quality assessment guidelines. Of the 965 initial articles retrieved, 34 were considered suitable for qualitative synthesis after screening procedures and removing duplicates and irrelevant records. There appears to be an imbalance between the general public’s low and dentists’ high awareness. This draws attention to the shortage of professional and societal knowledge-sharing and education initiatives. Women were more than twice as likely as men to have oral piercings. Piercing usage had a low incidence among a cohort of students with a mean age of 16. Merely circumstantial evidence has indicated a plausible correlation between oral and perioral piercings and the emergence of secondary bacterial and fungal colonization, particularly periodontopathogenic bacteria and Candida albicans. Furthermore, several adverse consequences have been observed linked to various piercings—such as lip and tongue piercings. These include caries, gingivitis/periodontitis, dental fractures, enamel chipping/cracks, plaque buildup, bone loss, bleeding, inflammation, and swelling. Given the risks involved and the complications that might impair oral health, the prevalence of oral piercings is alarming. As a result, public health authorities need to firmly support initiatives to raise awareness of the risks associated with oral/perioral piercings. For piercers to enhance their expertise in this field, professional training is necessary because there is a shortage of knowledge on the possible adverse effects of piercings.
Hardware-Based Methods for Electronic Device Protection against Invasive and Non-Invasive Attacks
Authors: Vidaković Marin , Vinko Davor
Published: November 2023

This paper reviews hardware-based protection methods for electronic devices, encompassing scientific publications and published patents. This review covers insights from the scientific community and innovative solutions patented in the industry. By combining these two sources, this paper offers a comprehensive and holistic review of electronic device security. Electronic devices are integral to modern life, but their widespread use invites security threats, both digital and physical. This paper reviews hardware-based protection methods against invasive and non-invasive attacks, emphasizing the importance of a dual approach through hardware design. Invasive attacks involve physical tampering, and we explore anti-tampering techniques such as conductive meshes, sensors and physically unclonable functions (PUFs). Non-invasive, side-channel attacks encompass various attack vectors, focusing on electromagnetic analysis. To counter these attacks, we analyze techniques like reducing and masking electromagnetic radiation. This paper bridges the gap between invasive and non-invasive attack mitigation. It underscores the necessity of a multifaceted approach to safeguard electronic devices in an interconnected world, preserving their reliability and functionality.
Business Purchase Prediction Based on XAI and LSTM Neural Networks
Authors: Predić Bratislav , Ćirić Milica , Stoimenov Leonid
Published: November 2023

The black-box nature of neural networks is an obstacle to the adoption of systems based on them, mainly due to a lack of understanding and trust by end users. Providing explanations of the model’s predictions should increase trust in the system and make peculiar decisions easier to examine. In this paper, an architecture of a machine learning time series prediction system for business purchase prediction based on neural networks and enhanced with Explainable artificial intelligence (XAI) techniques is proposed. The architecture is implemented on an example of a system for predicting the following purchases for time series using Long short-term memory (LSTM) neural networks and Shapley additive explanations (SHAP) values. The developed system was evaluated with three different LSTM neural networks for predicting the next purchase day, with the most complex network producing the best results across all metrics. Explanations generated by the XAI module are provided with the prediction results to the user to allow him to understand the system’s decisions. Another benefit of the XAI module is the possibility to experiment with different prediction models and compare input feature effects.
Genome-Wide Analysis and Expression Profiling of YUCCA Gene Family in Developmental and Environmental Stress Conditions in Tea Plant (Camellia sinensis)
Authors: Zhang Liping , Jin Shan , Bai Peixian , Ge Shibei , Yan Peng , Li Zhengzhen , Zhang Lan , Han Wenyan , Zeng Jianming , Li Xin
Published: November 2023

The tea plant is a perennial leaf-used economical crop and cultivated all over the world. Indole-3-acetic acid (IAA) plays key roles in plant development and environmental stress. YUCCA (YUC) flavin monooxygenases are the rate-limiting enzymes of the TAA/YUC pathway, which is the most important IAA biosynthetic pathway in plants. The YUC gene family in tea plants has not been systematically studied so far. A total of 17 CsYUC members were identified from a tea plant genome database and phylogenetically classified into three subfamilies. Phylogenetic analysis showed that the CsYUC gene family is evolutionarily conserved. The physical and chemical properties, gene structures, and conserved domains were analyzed. The expression profiles of CsYUCs were analyzed on the basis of open available RNA-seq data, as well as by RNA-seq and qRT-PCR assays. Combined with previous studies, it can be concluded that YUC10 may play key roles in seed development. The results also showed that CsYUC2.1 may play important roles in the coordinated regulation of the growth of leaf buds and flower buds induced by pruning. Low temperature markedly induced the expression of CsYUC2.2, -11.8, and -11.9. Furthermore, CsYUC genes that might play key roles in the specific development stages and involve enhancing the resistance to drought and NaCl stress were screened, respectively. This study could provide a research basis for deeply studying the gene functions of the CsYUC family in the tea plant.
Correction: Rheu et al. Effect of Fermented Sarco Oyster (Crassostrea gigas) Extract on Muscle Strength Enhancement in Postmenopausal Females: A Randomized, Double-Blind, Placebo-Controlled Trial. Int. J. Environ. Res. Public Health 2022, 19, 16450
Authors: Rheu Kyoung-Min , Lee Bae-Jin , Son Woo-Hyeon , Kim Dong-Seok , Park Hyun-Tae , Ha Min-Seong , Gong Byong-Hak , Jeon Byeong-Hwan
Published: November 2023

There was an error in the original publication [...]
Compromised Muscle Properties in a Severe Hypophosphatasia Murine Model
Authors: Pendleton Emily G. , Nichenko Anna S. , Mcfaline-Figueroa Jennifer , Raymond-Pope Christiana J. , Schifino Albino G. , Pigg Taylor M. , Barrow Ruth P. , Greising Sarah M. , Call Jarrod A. , Mortensen Luke J.
Published: November 2023

Hypophosphatasia (HPP) is a rare metabolic bone disorder characterized by low levels of tissue non-specific alkaline phosphatase (TNAP) that causes under-mineralization of the bone, leading to bone deformity and fractures. In addition, patients often present with chronic muscle pain, reduced muscle strength, and an altered gait. In this work, we explored dynamic muscle function in a homozygous TNAP knockout mouse model of severe juvenile onset HPP. We found a reduction in skeletal muscle size and impairment in a range of isolated muscle contractile properties. Using histological methods, we found that the structure of HPP muscles was similar to healthy muscles in fiber size, actin and myosin structures, as well as the α-tubulin and mitochondria networks. However, HPP mice had significantly fewer embryonic and type I fibers than wild type mice, and fewer metabolically active NADH+ muscle fibers. We then used oxygen respirometry to evaluate mitochondrial function and found that complex I and complex II leak respiration were reduced in HPP mice, but that there was no disruption in efficiency of electron transport in complex I or complex II. In summary, the severe HPP mouse model recapitulates the muscle strength impairment phenotypes observed in human patients. Further exploration of the role of alkaline phosphatase in skeletal muscle could provide insight into mechanisms of muscle weakness in HPP.
Establishing a Diagnosis of Pulmonary Sarcoidosis
Authors: Grutters Jan C.
Published: November 2023

Pulmonary sarcoidosis is the most prevalent manifestation of sarcoidosis and the commonest diagnosis in clinics for ILD. Due to the lack of a simple and reliable test, making the diagnosis is often challenging. There are three criteria that must always be considered: (1) compatible clinical presentation; (2) evidence of granuloma formation (usually non-caseating); and (3) exclusion of alternative causes of granulomatous disease. There are various tools available for diagnosis, amongst which serum biomarkers like sACE and sIL-2R, HRCT, BAL, EBUS/EUS and sometimes bronchoscopic or surgical lung biopsy are most contributive. However, the degree of invasiveness of the applied test and associated risk to the patient must be weighed against management consequences. In specific situations (e.g., presentation as Löfgren’s syndrome) or when there is high suspicion based on HRCT in the context of supportive clinical findings, it might be justifiable to decide on a “working diagnosis of sarcoidosis” and to refrain from further invasive procedures for the patient. This should, however, preferably be agreed upon after discussion in an experienced multidisciplinary team and requires close follow-up of the patient. In general, it is advisable to always maintain a healthy dose of skepticism when making the diagnosis of sarcoidosis, especially when the clinical course of disease gives rise to this.
The Prognostic Role of Platelet-to-Lymphocyte Ratio in Acute Coronary Syndromes: A Systematic Review and Meta-Analysis
Authors: Pruc Michal , Peacock Frank William , Rafique Zubaid , Swieczkowski Damian , Kurek Krzysztof , Tomaszewska Monika , Katipoglu Burak , Koselak Maciej , Cander Basar , Szarpak Lukasz
Published: November 2023

This study aimed to investigate the potential prognostic role of the platelet-to-lymphocyte (PLR) ratio in patients presenting with suspected acute coronary syndromes (ACS). A systematic search of PubMed Central, Scopus, EMBASE, and the Cochrane Library from conception through 20 August 2023 was conducted. We used odds ratios (OR) as the effect measure with 95% confidence intervals (CIs) for dichotomous data and mean differences (MD) with a 95% CI for continuous data. If I2 was less than 50% or the p value of the Q tests was less than 0.05, a random synthesis analysis was conducted. Otherwise, a fixed pooled meta-analysis was performed. Nineteen studies fulfilled the eligibility criteria and were included in the meta-analysis. PLR was higher in MACE-positive (164.0 ± 68.6) than MACE-negative patients (115.3 ± 36.9; MD = 40.14; 95% CI: 22.76 to 57.52; p < 0.001). Pooled analysis showed that PLR was higher in AMI patients who died (183.3 ± 30.3), compared to survivors (126.2 ± 16.8; MD = 39.07; 95% CI: 13.30 to 64.84; p = 0.003). It was also higher in the ACS vs. control group (168.2 ± 81.1 vs. 131.9 ± 37.7; MD = 39.01; 95% CI: 2.81 to 75.21; p = 0.03), STEMI vs. NSTEMI cohort (165.5 ± 92.7 vs. 159.5 ± 87.8; MD = 5.98; 95% CI: −15.09 to 27.04; p = 0.58), and MI vs. UAP populations (162.4 ± 90.0 vs. 128.2 ± 64.9; MD = 18.28; 95% CI: −8.16 to 44.71; p = 0.18). Overall, our findings confirmed the potential prognostic role of the plate-let-to-lymphocyte (PLR) ratio in patients presenting with suspected acute coronary syndromes (ACS). Its use as a risk stratification tool should be examined prospectively to define its capability for evaluation in cardiovascular patients.
Monitoring Cochlear Nerve Action Potential for Hearing Preservation in Medium/Large Vestibular Schwannoma Surgery: Tips and Pitfalls
Authors: Hochet Baptiste , Daoudi Hannah , Lefevre Etienne , Nguyen Yann , Bernat Isabelle , Sterkers Olivier , Lahlou Ghizlene , Kalamarides Michel
Published: November 2023

The diagnosis of large vestibular schwannomas (VS) with retained useful hearing has become increasingly common. Preservation of facial nerve (FN) function has improved using intraoperative EMG monitoring, hearing preservation remains challenging, with the recent use of cochlear nerve action potential (CNAP) monitoring. This prospective longitudinal series of VS with useful hearing operated on using a retrosigmoid approach included 37 patients with a mean largest extrameatal VS. diameter of 25 ± 8.7 mm (81% of Koos stage 4). CNAP was detected in 51% of patients, while auditory brainstem responses (ABR) were present in 22%. Patients were divided into two groups based on the initial intraoperative CNAP status, whether it was present or absent. FN function was preserved (grade I–II) in 95% of cases at 6 months. Serviceable hearing (class A + B) was preserved in 16% of the cases, while 27% retained hearing with intelligibility (class A–C). Hearing with intelligibility (class A–C) was preserved in 42% of cases when CNAP could be monitored in the early stages of VS resection versus 11% when it was initially absent. Changes in both the approach to the cochlear nerve and VS resection are mandatory in preserving CNAP and improve the rate of hearing preservation.
Enhancing Sumoylation Site Prediction: A Deep Neural Network with Discriminative Features
Authors: Khan Salman , Khan Mukhtaj , Iqbal Nadeem , Dilshad Naqqash , Almufareh Maram Fahaad , Alsubaie Najah
Published: November 2023

Sumoylation is a post-translation modification (PTM) mechanism that involves many critical biological processes, such as gene expression, localizing and stabilizing proteins, and replicating the genome. Moreover, sumoylation sites are associated with different diseases, including Parkinson’s and Alzheimer’s. Due to its vital role in the biological process, identifying sumoylation sites in proteins is significant for monitoring protein functions and discovering multiple diseases. Therefore, in the literature, several computational models utilizing conventional ML methods have been introduced to classify sumoylation sites. However, these models cannot accurately classify the sumoylation sites due to intrinsic limitations associated with the conventional learning methods. This paper proposes a robust computational model (called Deep-Sumo) for predicting sumoylation sites based on a deep-learning algorithm with efficient feature representation methods. The proposed model employs a half-sphere exposure method to represent protein sequences in a feature vector. Principal Component Analysis is applied to extract discriminative features by eliminating noisy and redundant features. The discriminant features are given to a multilayer Deep Neural Network (DNN) model to predict sumoylation sites accurately. The performance of the proposed model is extensively evaluated using a 10-fold cross-validation test by considering various statistical-based performance measurement metrics. Initially, the proposed DNN is compared with the traditional learning algorithm, and subsequently, the performance of the Deep-Sumo is compared with the existing models. The validation results show that the proposed model reports an average accuracy of 96.47%, with improvement compared with the existing models. It is anticipated that the proposed model can be used as an effective tool for drug discovery and the diagnosis of multiple diseases.
Research on the Recrystallization Process of the Ti-70 Titanium Alloy Sheet
Authors: Zhang Zhixin , Tang Bin , Li Ruifeng , Fan Jiangkun , Li Jinshan
Published: November 2023

As Ti-70 is a new type of marine titanium alloy, research on the recrystallization process of its sheet is necessary. This article studies the effects of different temperatures and times of annealing on the recrystallization process of 5.0 mm thick Ti-70 titanium alloy cold-rolled sheets by metallographic analyses and hardness tests. The results show that after 30 min of annealing at 620~700 °C, the recrystallization process was mostly complete, and uniform and equiaxed recrystallized grains could be obtained. The recrystallization process starts after 8 min of annealing at 700 °C, and after holding for 15~30 min, the recrystallization process is almost complete and the grain size is about 8.2 μm. The recrystallization activation energy of a Ti-70 titanium alloy cold-rolled sheet is Qr = 11.0645 × 104 J/mol. The ultimate tensile strength (Rm) can be controlled between 705 and 852 MPa, the yield strength (Rp0.2) can be controlled between approximately 623 and 793 MPa, and the elongation percentage (A) can be controlled between approximately 10.0 and 25.0% after rolling and heat treatment of Ti-70 alloy sheets.
Enhanced Activity and Stability of an Acetyl Xylan Esterase in Hydrophilic Alcohols through Site-Directed Mutagenesis
Authors: Madubuike Henry , Ferry Natalie
Published: November 2023

Current demands for the development of suitable biocatalysts showing high process performance is stimulated by the need to replace current chemical synthesis with cleaner alternatives. A drawback to the use of biocatalysts for unique applications is their low performance in industrial conditions. Hence, enzymes with improved performance are needed to achieve innovative and sustainable biocatalysis. In this study, we report the improved performance of an engineered acetyl xylan esterase (BaAXE) in a hydrophilic organic solvent. The structure of BaAXE was partitioned into a substrate-binding region and a solvent-affecting region. Using a rational design approach, charged residues were introduced at protein surfaces in the solvent-affecting region. Two sites present in the solvent-affecting region, A12D and Q143E, were selected for site-directed mutagenesis, which generated the mutants MUT12, MUT143 and MUT12-143. The mutants MUT12 and MUT143 reported lower Km (0.29 mM and 0.27 mM, respectively) compared to the wildtype (0.41 mM). The performance of the mutants in organic solvents was assessed after enzyme incubation in various strengths of alcohols. The mutants showed improved activity and stability compared to the wild type in low strengths of ethanol and methanol. However, the activity of MUT143 was lost in 40% methanol while MUT12 and MUT12-143 retained over 70% residual activity in this environment. Computational analysis links the improved performance of MUT12 and MUT12-143 to novel intermolecular interactions that are absent in MUT143. This work supports the rationale for protein engineering to augment the characteristics of wild-type proteins and provides more insight into the role of charged residues in conferring stability.
Grey Correlation Analysis of Drying Characteristics and Quality of Hypsizygus marmoreus (Crab-Flavoured Mushroom) By-Products
Authors: Lai Pufu , Xiao Zheng , Li Yibin , Tang Baosha , Wu Li , Weng Minjie , Sun Junzheng , Chen Junchen
Published: November 2023

The physical properties and nutritional quality of H. marmoreus by-products (HMB) dried by different methods were comprehensively evaluated by a rigorous statistical method of grey correlation analysis. The results indicated that different drying methods had significant impacts on the characteristics of HMB. Heat pump drying (HPD) was conducive to the preservation of protein and reducing sugar, and hot air drying (HAD) maintained a high content of total flavonoids. The highest fat, polysaccharide, and total phenolic contents were obtained by heated vacuum freeze-drying (H-VFD) treatment. The unheated vacuum freeze-drying (UH-VFD) treatment achieved bright colour, lacunose texture profile, and looser organization structure. The grey correlation analysis showed that UH-VFD and H-VFD had higher-weighted correlation degrees than HPD and HAD. HMB had many higher nutritional components than commodity specifications, especially protein, fat, polyphenols, and amino acids, and had potential applications in the food industry as functional foods and nutraceutical agents.
Design of a Novel Sericite–Phosphoric Acid Framework for Enhancement of Pb(II) Adsorption
Authors: Kim Han-Soo , Choi Hee-Jeong
Published: November 2023

In this study, phosphoric acid was used to attach anions to the weak interlayer structure of sericite, one of the clay minerals composed of a tetrahedral structure of silicate, to increase the adsorption capacity of cations. Natural sericite beads (NSB) and activated sericite beads with phosphoric acid (PSB) were prepared as beads in order to increase reusability and facilitate the separation of adsorbates and adsorbents. Using this, lead (Pb(II)) removal efficiency from an aqueous solution was comparatively analyzed. The pHpzc was 6.43 in NSB but lowered to 3.96 in PSB, confirming that more acidic functional groups were attached to the PSB surface. According to FT-IR analysis, P=O, P-O-C, P=OOH and P-O-P bonds appeared on the surface of the PSB adsorbent, and the peaks of carboxyl groups and OH-groups were large and broad. The maximum adsorption capacity of Langmuir was 52.08 mg/g for NSB and 163.93 mg/g for PSB. The adsorption process was close to physical adsorption for NSB and chemical adsorption for PSB, and both adsorbents were endothermic reactions in nature in that the higher the temperature, the higher the adsorption efficiency. The adsorption mechanism of Pb(II) to PSB was achieved by ion exchange, electrostatic interaction, hydrogen bonding, and complexation. The adsorption of Pb(II) using PSB was not significantly affected by the adsorption of competing ions and showed a high adsorption efficiency of 94% in reuse up to 6 times. This confirms the favorable feasibility of removing Pb(II) from industrial wastewater using PSB.
Influence of Co3O4 Nanostructure Morphology on the Catalytic Degradation of p-Nitrophenol
Authors: Chen Huihui , Yang Mei , Liu Yuan , Yue Jun , Chen Guangwen
Published: November 2023

The design and fabrication of nanomaterials with controllable morphology and size is of critical importance to achieve excellent catalytic performance in heterogeneous catalysis. In this work, cobalt oxide (Co3O4) nanostructures with different morphologies (nanoplates, microflowers, nanorods and nanocubes) were successfully constructed in order to establish the morphology–property–performance relationship of the catalysts. The morphology and structure of the nanostructured Co3O4 were characterized by various techniques, and the catalytic performance of the as-prepared nanostructures was studied by monitoring the reduction of p-nitrophenol to p-aminophenol in the presence of excess NaBH4. The catalytic performance was found to be strongly dependent on their morphologies. The experimental results show that the pseudo-first-order reaction rate constants for Co3O4 nanostructures with various shapes are, respectively, 1.49 min−1 (nanoplates), 1.40 min−1 (microflowers), 0.78 min−1 (nanorods) and 0.23 min−1 (nanocubes). The Co3O4 nanoplates exhibited the highest catalytic activity among the four nanostructures, due to their largest specific surface area, relatively high total pore volume, best redox properties and abundance of defect sites. The established correlation between morphology, property and catalytic performance in this work will offer valuable insight into the design and application of nanostructured Co3O4 as a potential non-noble metal catalyst for p-nitrophenol reduction.
Cleomin Exerts Acute Antinociceptive Effects in Mice via GABAB and Muscarinic Receptors
Authors: Opretzka Luíza Carolina França , Viana Max Denisson Maurício , de Lima Alyne Almeida , de Souza Thalisson Amorim , Scotti Marcus Tullius , Tavares Josean Fechine , da Silva Marcelo Sobral , Soares Milena Botelho Pereira , Villarreal Cristiane Flora
Published: November 2023

Cleomin, a 1,3-oxazolidine-2-thione, was recently isolated from Neocalyptrocalyx longifolium, a species traditionally used for treating painful conditions. Reports about the pharmacological activities of cleomin are lacking. Here, the antinociceptive effects of cleomin were investigated using mice models of pain, namely the formalin, the cold plate, and the tail flick tests. Motor integrity was assessed in the rota-rod test. Antagonism assays and in silico docking analyses were performed to investigate the putative mechanisms of action. Cleomin (12.5–25 mg/kg), at doses that did not induce motor impairment, induced dose-dependent antinociception in both early and late phases of the formalin test and reduced nociceptive behaviors in both the cold plate and tail flick tests. Pretreatments with phaclofen and atropine attenuated the antinociceptive effects of cleomin, implicating the involvement of GABAB and muscarinic receptors. In silico docking studies suggested satisfactory coupling between cleomin and GABAB and M2 receptors, hence corroborating their role in cleomin’s activity. Pretreatments with naloxone, yohimbine, bicuculline, and methysergide did not affect the antinociception of cleomin. In silico pharmacokinetics prediction showed a good drug ability profile of cleomin. In conclusion, cleomin promoted antinociception mediated by GABAB and muscarinic receptors. These findings support further investigation of the analgesic potential of cleomin.