bluuebunny/crossref_metadata_2025 huggingface dataset:
Prevalence of Flavescence Dorée Phytoplasma-Infected Scaphoideus titanus in Different Vineyard Agroecosystems of Northwestern Italy
Authors: Ripamonti Matteo , Pegoraro Mattia , Rossi Marika , Bodino Nicola , Beal Dylan , Panero Loretta , Marzachì Cristina , Bosco Domenico
Published: May 2020

Quantitative estimates of vector populations and their infectivity in the wild and in cultivated compartments of agroecosystems have been carried out to elucidate the role of the wild compartment in the epidemiology of Flavescence dorée (FD). Seven sites were selected for the investigations in the Piedmont Region of Italy. They were characterized by a high variety of agricultural and ecological landscape features, and included a vineyard surrounded by wild vegetation. In order to describe abundance and prevalence of FD-infected vectors in the cultivated and wild compartments of the vineyard agroecosystem, adults of Scaphoideus titanus were collected by yellow sticky traps inside and outside the vineyard over the period July 10th–September 9th, 2015. They were counted and singly analyzed for the presence of FD phytoplasmas by PCR. Multifactorial correlations among vector population level, prevalence of infected insects inside and outside the vineyards, disease prevalence in cultivated and wild Vitis plants, and location of wild Vitis plants with respect to the vineyard were analyzed. Abundance of S. titanus adults significantly decreased from the end of July onwards, particularly inside the vineyard (average range 22.7 ± 2.5 insects/trap). Percentage of FD-positive S. titanus was significantly higher outside the vineyard (up to 48% on average) compared to inside the vineyard (up to 34% on average), and increased during the season in both compartments.
New Insight into the Systematics of European Lepidocyrtus (Collembola: Entomobryidae) Using Molecular and Morphological Data
Authors: Winkler Daniel , Mateos Eduardo , Traser György , Lakatos Ferenc , Tóth Viktória
Published: May 2020

The Collembolan genus Lepidocyrtus is subdivided into up to eight subgenera, of which only Lepidocyrtus s.str. (Bourlet, 1839) and Lanocyrtus (Yoshii & Suhardjono, 1989) are represented by European species. The discovery of unique characters in the European species Lepidocyrtus tomosvaryi (rounded dental tubercle) and L. peisonis (lateral tuft of long filiform chaetae in abdomen III) has only described so far for species of the subgenera Setogaster (Salmon, 1951) and Cinctocyrtus (Yoshii & Suhardjono, 1989) and has raised the need to perform a molecular analysis by involving other representative species of the genus. For this study, phylogenetic analysis of 15 Lepidocyrtus species occurring in the Carpathian Basin were carried out. The analyses, which was based on both concatenated datasets of COII and EF1-α sequences and individual gene sequences, clearly placed L. tomosvaryi within the subgenus Lanocyrtus and L. peisonis within Lepidocyrtus s.srt. European species groups defined on the basis of morphological characters were only partly confirmed by the concatenated and COII analyses because of the splitting of the pallidus–serbicus-group, whereas EF1- α sequences weakly supported this group.
Comparative Ecology of Hyalomma lusitanicum and Hyalomma marginatum Koch, 1844 (Acarina: Ixodidae)
Authors: Valcárcel Félix , González Julia , González Marta G. , Sánchez María , Tercero José María , Elhachimi Latifa , Carbonell Juan D. , Olmeda A. Sonia
Published: May 2020

The genus Hyalomma belongs to the Ixodidae family and includes many tick species. Most species in this genus are African species, but two of them, Hyalomma lusitanicum and Hyalomma marginatum, are also found in Europe and, owing to their morphological similarity, it is very difficult to tell them apart. This is a major concern because their phenology and vector capacities are quite different. Moreover, they share many habitats and both are currently spreading to new areas, probably due to climate change and animal/human movements. In this study, we review the described ecology of the two species and provide further interesting data on H. lusitanicum based on the authors’ experience, which could be useful in assessing the risk they pose to humans and animals.
Asteraceae Paradox: Chemical and Mechanical Protection of Taraxacum Pollen
Authors: Vanderplanck Maryse , Gilles Hélène , Nonclercq Denis , Duez Pierre , Gerbaux Pascal
Published: May 2020

Excessive pollen harvesting by bees can compromise the reproductive success of plants. Plants have therefore evolved different morphological structures and floral cues to narrow the spectrum of pollen feeding visitors. Among “filtering” mechanisms, the chemical and mechanical protection of pollen might shape bee-flower interactions and restrict pollen exploitation to a specific suite of visitors such as observed in Asteraceae. Asteraceae pollen is indeed only occasionally exploited by generalist bee species but plentifully foraged by specialist ones (i.e., Asteraceae paradox). During our bioassays, we observed that micro-colonies of generalist bumblebee (Bombus terrestris L.) feeding on Taraxacum pollen (Asteraceae) reduced their pollen collection and offspring production. Bees also experienced physiological effects of possible defenses in the form of digestive damage. Overall, our results suggest the existence of an effective chemical defense in Asteraceae pollen, while the hypothesis of a mechanical defense appeared more unlikely. Pre- and post-ingestive effects of such chemical defenses (i.e., nutrient deficit or presence of toxic compounds), as well as their role in the shaping of bee-flower interactions, are discussed. Our results strongly suggest that pollen chemical traits may act as drivers of plant selection by bees and partly explain why Asteraceae pollen is rare in generalist bee diets.
Transcriptomic Analysis of Mating Responses in Bemisia tabaci MED Females
Authors: Huo Zhijia , Liu Yating , Yang Jinjian , Xie Wen , Wang Shaoli , Wu Qingjun , Zhou Xuguo , Pang Baoping , Zhang Youjun
Published: May 2020

Mating triggers substantial changes in gene expression and leads to subsequent physiological and behavioral modifications. However, postmating transcriptomic changes responding to mating have not yet been fully understood. Here, we carried out RNA sequencing (RNAseq) analysis in the sweet potato whitefly, Bemisia tabaci MED, to identify genes in females in response to mating. We compared mRNA expression in virgin and mated females at 24 h. As a result, 434 differentially expressed gene transcripts (DEGs) were identified between the mated and unmated groups, including 331 up- and 103 down-regulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that many of these DEGs encode binding-related proteins and genes associated with longevity. An RT-qPCR validation study was consistent with our transcriptomic analysis (14/15). Specifically, expression of P450s (Cyp18a1 and Cyp4g68), ubiquitin-protein ligases (UBR5 and RNF123), Hsps (Hsp68 and Hsf), carboxylase (ACC-2), facilitated trehalose transporters (Tret1-2), transcription factor (phtf), and serine-protein kinase (TLK2) were significantly elevated in mated females throughout seven assay days. These combined results offer a glimpe of postmating molecular modifications to facilitate reproduction in B. tabaci females.
CD56bright Natural Killer Cells: A Possible Biomarker of Different Treatments in Multiple Sclerosis
Authors: Laroni Alice , Uccelli Antonio
Published: May 2020

Multiple sclerosis (MS) is an immune-mediated disease of the central nervous system, which leads, in many cases, to irreversible disability. More than 15 disease-modifying treatments (DMTs) are available for the treatment of MS. Clinical activity or activity at magnetic resonance imaging (MRI) are now used to assess the efficacy of DMTs, but are negative prognostic factors per se. Therefore, a biomarker permitting us to identify patients who respond to treatment before they develop clinical/radiological signs of MS activity would be of high importance. The number of circulating CD56bright natural killer (NK) cells may be such a biomarker. CD56bright NK cells are a regulatory immune population belonging to the innate immune system. The number of CD56bright NK cells increases upon treatment with interferon-beta, alemtuzumab, dimethyl fumarate, after autologous hematopoietic stem cell transplantation, and is higher in those who respond to fingolimod. In some cases, an increased number of CD56bright NK cells is associated with an increase in their regulatory function. In the current review, we will evaluate the known effect on CD56bright NK cells of DMTs for MS, and will discuss their possible role as a biomarker for treatment response in MS.
Neuroendocrine Cancer of the Breast: A Rare Entity
Authors: Irelli Azzurra , Sirufo Maria Maddalena , Morelli Luca , D’Ugo Carlo , Ginaldi Lia , De Martinis Massimo
Published: May 2020

Neuroendocrine breast cancer (NEBC) is a rare histotype of breast carcinoma that presents, in most cases, positive hormone receptors and negative HER2. Indeed, the analysis of gene expression profiles revealed that NEBC belongs mainly to the luminal subtype. Cases of HER2-positive and triple-negative NEBC are rare. The cardinal treatment of early NEBC is surgery, similar to the treatment of invasive non-special histological type carcinoma. The use of radiotherapy follows the criteria applied in infiltrating breast cancer of non-special histotype. In the post-operative phase, therefore after the surgical treatment of mammary quadrantectomy, or mastectomy associated with homolateral sentinel lymph node removal ± axillary dissection, based on the histopathological characteristics of the tumor, the use of chemotherapy (anthracycline + taxane) and/or hormone therapy, whether or not associated with anti-HER2 therapy (trastuzumab) is the rule. Literature data report the use of cisplatin and etoposide, as in small cell lung cancers. Most of the information currently available derive from single case reports or a series of clinical cases; it follows the difficulty of formulating definite recommendations on the correct management of this histological type of breast cancer. This review describes available knowledge on this rare entity to improve the diagnostic and therapeutic strategies and offer insights to stimulate exploration of the many aspects still unknown.
Alcohol Hangover Does Not Alter the Application of Model-Based and Model-Free Learning Strategies
Authors: Berghäuser Julia , Bensmann Wiebke , Zink Nicolas , Endrass Tanja , Beste Christian , Stock Ann-Kathrin
Published: May 2020

Frequent alcohol binges shift behavior from goal-directed to habitual processing modes. This shift in reward-associated learning strategies plays a key role in the development and maintenance of alcohol use disorders and seems to persist during (early stages of) sobriety in at-risk drinkers. Yet still, it has remained unclear whether this phenomenon might be associated with alcohol hangover and thus also be found in social drinkers. In an experimental crossover design, n = 25 healthy young male participants performed a two-step decision-making task once sober and once hungover (i.e., when reaching sobriety after consuming 2.6 g of alcohol per estimated liter of total body water). This task allows the separation of effortful model-based and computationally less demanding model-free learning strategies. The experimental induction of alcohol hangover was successful, but we found no significant hangover effects on model-based and model-free learning scores, the balance between model-free and model-based valuation (ω), or perseveration tendencies (π). Bayesian analyses provided positive evidence for the null hypothesis for all measures except π (anecdotal evidence for the null hypothesis). Taken together, alcohol hangover, which results from a single binge drinking episode, does not impair the application of effortful and computationally costly model-based learning strategies and/or increase model-free learning strategies. This supports the notion that the behavioral deficits observed in at-risk drinkers are most likely not caused by the immediate aftereffects of individual binge drinking events.
Benefits of Adhering to a Mediterranean Diet Supplemented with Extra Virgin Olive Oil and Pistachios in Pregnancy on the Health of Offspring at 2 Years of Age. Results of the San Carlos Gestational Diabetes Mellitus Prevention Study.
Authors: Melero Verónica , Assaf-Balut Carla , Torre Nuria García de la , Jiménez Inés , Bordiú Elena , Valle Laura del , Valerio Johanna , Familiar Cristina , Durán Alejandra , Runkle Isabelle , Miguel María Paz de , Montañez Carmen , Barabash Ana , Cuesta Martín , Herraiz Miguel A. , Izquierdo Nuria , Rubio Miguel A. , Calle-Pascual Alfonso L.
Published: May 2020

The intrauterine environment may be related to the future development of chronic diseases in the offspring. The St. Carlos gestational diabetes mellitus (GDM) prevention study, is a randomized controlled trial that evaluated the influence of the early (before 12th gestational week) Mediterranean diet (MedDiet) on the onset of GDM and adverse gestational outcomes. Out of 874 women assessed after delivery (440 control group (CG)/434 intervention group (IG)), 703 children were followed (365/338; CG/IG), with the aim to assess whether the adherence to a MedDiet during pregnancy induces health benefits for the offspring during the first two years of life. Logistic regression analysis showed that the IG in children of mothers with pre-gestational body mass index (BMI) < 25 kg/m2 and normal glucose tolerance (NGT), was associated with a lower risk (RR(95% CI)) of suffering from severe events requiring hospitalization due to bronchiolitis/asthma (0.75(0.58–0.98) and 0.77(0.59–0.99), respectively) or other diseases that required either antibiotic (0.80(0.65–0.98) and 0.80(0.65–0.99), respectively), corticosteroid treatment (0.73(0.59–0.90) and 0.79(0.62–1.00) respectively) or both (all p < 0.05). A nutritional intervention based on the MedDiet during pregnancy is associated with a reduction in offspring’s hospital admissions, especially in women with pre-gestational BMI < 25 kg/m2 and NGT.
Avascular Spaces of the Female Pelvis—Clinical Applications in Obstetrics and Gynecology
Authors: Kostov Stoyan , Slavchev Stanislav , Dzhenkov Deyan , Mitev Dimitar , Yordanov Angel
Published: May 2020

The term “spaces” refers to the areas delimited by at least two independent fasciae and filled with areolar connective tissue. However, there is discrepancy regarding the spaces and their limits between clinical anatomy and gynecologic surgery, as not every avascular space described in literature is delimited by at least two fasciae. Moreover, new spaces and surgical planes have been developed after the adoption of laparoscopy and nerve-sparing gynecological procedures. Avascular spaces are useful anatomical landmarks in retroperitoneal anatomic and pelvic surgery for both malignant and benign conditions. A noteworthy fact is that for various gynecological diseases, there are different approaches to the avascular spaces of the female pelvis. This is a significant difference, which is best demonstrated by dissection of these spaces for gynecological, urogynecological, and oncogynecological operations. Thorough knowledge regarding pelvic anatomy of these spaces is vital to minimize morbidity and mortality. In this article, we defined nine avascular female pelvic spaces—their boundaries, different approaches, attention during dissection, and applications in obstetrics and gynecology. We described the fourth space and separate the paravesical and pararectal space, as nerve-sparing gynecological procedures request a precise understanding of retroperitoneal spaces.
The Marker of Tubular Injury, Kidney Injury Molecule-1 (KIM-1), in Acute Kidney Injury Complicating Acute Pancreatitis: A Preliminary Study
Authors: Wajda Justyna , Dumnicka Paulina , Kolber Witold , Sporek Mateusz , Maziarz Barbara , Ceranowicz Piotr , Kuźniewski Marek , Kuśnierz-Cabala Beata
Published: May 2020

Acute pancreatitis (AP) may be associated with severe inflammation and hypovolemia leading to organ complications including acute kidney injury (AKI). According to current guidelines, AKI diagnosis is based on dynamic increase in serum creatinine, however, creatinine increase may be influenced by nonrenal factor and appears late following kidney injury. Kidney injury molecule-1 (KIM-1) is a promising marker of renal tubular injury and it has not been studied in AP. Our aim was to assess if urinary KIM-1 may be used to diagnose AKI complicating the early stage of AP. We recruited 69 patients with mild to severe AP admitted to a secondary care hospital during the first 24 h from initial symptoms of AP. KIM-1 was measured in urine samples collected on the day of admission and two subsequent days of hospital stay. AKI was diagnosed based on creatinine increase according to Kidney Disease: Improving Global Outcomes 2012 guidelines. Urinary KIM-1 on study days 1 to 3 was not significantly higher in 10 patients who developed AKI as compared to those without AKI and did not correlate with serum creatinine or urea. On days 2 and 3, urinary KIM-1 correlated positively with urinary liver-type fatty acid-binding protein, another marker of tubular injury. On days 2 and 3, urinary KIM-1 was higher among patients with systemic inflammatory response syndrome, and several correlations between KIM-1 and inflammatory markers (procalcitonin, urokinase-type plasminogen activator receptor, C-reactive protein) were observed on days 1 to 3. With a limited number of patients, our study cannot exclude the diagnostic utility of KIM-1 in AP, however, our results do not support it. We hypothesize that the increase of KIM-1 in AKI complicating AP lasts a short time, and it may only be observed with more frequent monitoring of the marker. Moreover, urinary KIM-1 concentrations in AP are associated with inflammation severity.
Unrealistic Optimism in the Time of Coronavirus Pandemic: May It Help to Kill, If So—Whom: Disease or the Person?
Authors: Dolinski Dariusz , Dolinska Barbara , Zmaczynska-Witek Barbara , Banach Maciej , Kulesza Wojciech
Published: May 2020

Objective: The results of numerous empirical studies have showed the occurrence of so-called unrealistic optimism. Thus, we aimed to investigate whether in the situation of an imminent coronavirus pandemic, people would still perceive themselves as being less exposed to the disease than others. Methods: Survey studies were conducted to examine the level of unrealistic optimism. Participants (n = 171, 67.3% of women) in a subjective way judged the risk of their coronavirus infection and the likelihood that this would happen to an average student of the same sex from their class. The survey was conducted in three waves: prior to the announcement of the first case of coronavirus (2–3 March), immediately after that announcement (5–6 March), and a few days later (9–10 March). Results: We showed that women estimated the chances of being infected as significantly higher (M = 4.52, SD = 2.079; t = 2.387; p = 0.018; Cohen’s d = 0.393) than men (M = 3.71, SD = 2.042). The phenomenon of unrealistic optimism was observed especially in men (as compared to other male participants) as it appeared in all three measures (M (you) = 3.95 vs. M (other male student) = 4.63; M = 3.71 vs. M = 4.68, and M = 4.46 vs. M = 5.38 in phase one, two, and three, respectively; p ≤ 0.006 for all comparison), but also in women in the last two measures (M (you) = 4.55 vs. M (other female student) = 4.95, and M = 4.99 vs. M = 5.38 in phase 2 and 3, respectively; p ≤ 0.012 for both comparisons). Conclusions: The study revealed a fairly general occurrence of unrealistic optimism, which was mainly observed in men as it appeared in all three measures, but also in women in the last two measures. This result is important for health experts who are responsible for making people comply with regulations concerning social distancing, putting masks on to stop infection, and staying at home. It is possible that unrealistically optimistic people will behave much less in line with the aforementioned recommendations, causing coronavirus to spread widely.
No Autopsies on COVID-19 Deaths: A Missed Opportunity and the Lockdown of Science
Authors: Salerno Monica , Sessa Francesco , Piscopo Amalia , Montana Angelo , Torrisi Marco , Patanè Federico , Murabito Paolo , Li Volti Giovanni , Pomara Cristoforo
Published: May 2020

Background: The current outbreak of COVID-19 infection, which started in Wuhan, Hubei province, China, in December 2019, is an ongoing challenge and a significant threat to public health requiring surveillance, prompt diagnosis, and research efforts to understand a new, emergent, and unknown pathogen and to develop effective therapies. Despite the increasing number of published studies on COVID-19, in all the examined studies the lack of a well-defined pathophysiology of death among patients who died following COVID-19 infection is evident. Autopsy should be considered mandatory to define the exact cause of death, thus providing useful clinical and epidemiologic information as well as pathophysiological insights to further provide therapeutic tools. Methods: A literature review was performed on PubMed database, using the key terms: “COVID-19”, “nCov 19”, and “Sars Cov 2”. 9709 articles were retrieved; by excluding all duplicated articles, additional criteria were then applied: articles or abstracts in English and articles containing one of the following words: “death”, “died”, “comorbidity”, “cause of death”, “biopsy”, “autopsy”, or “pathological”. Results: A total of 50 articles met the inclusion criteria. However, only 7 of these studies reported autopsy-based data. Discussion: The analysis of the main data from the selected studies concerns the complete analysis of 12,954 patients, of whom 2269 died (with a mortality rate of 17.52%). Laboratory confirmation of COVID-19 infection was obtained in all cases and comorbidities were fully reported in 46 studies. The most common comorbidities were: cardiovascular diseases (hypertension and coronary artery disease), metabolic disorders (diabetes, overweight, or obesity), respiratory disorders (chronic obstructive pulmonary disease), and cancer. The most common reported complications were: acute respiratory distress syndrome (ARDS), acute kidney injury, cardiac injury, liver insufficiency, and septic shock. Only 7 papers reported histological investigations. Nevertheless, only two complete autopsies are described and the cause of death was listed as COVID-19 in only one of them. The lack of postmortem investigation did not allow a definition of the exact cause of death to determine the pathways of this infection. Based on the few histopathological findings reported in the analyzed studies, it seems to be a clear alteration of the coagulation system: frequently prothrombotic activity with consequent thromboembolism was described in COVID-19 patients. As a scientific community, we are called on to face this global threat, and to defeat it with all the available tools necessary. Despite the improvement and reinforcement of any method of study in every field of medicine and science, encouraging the autopsy practice as a tool of investigation could also therefore, help physicians to define an effective treatment to reduce mortality.
Highly Conserved Homotrimer Cavity Formed by the SARS-CoV-2 Spike Glycoprotein: A Novel Binding Site
Authors: Kalathiya Umesh , Padariya Monikaben , Mayordomo Marcos , Lisowska Małgorzata , Nicholson Judith , Singh Ashita , Baginski Maciej , Fahraeus Robin , Carragher Neil , Ball Kathryn , Haas Juergen , Daniels Alison , Hupp Ted R. , Alfaro Javier Antonio
Published: May 2020

An important stage in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) life cycle is the binding of the spike (S) protein to the angiotensin converting enzyme-2 (ACE2) host cell receptor. Therefore, to explore conserved features in spike protein dynamics and to identify potentially novel regions for drugging, we measured spike protein variability derived from 791 viral genomes and studied its properties by molecular dynamics (MD) simulation. The findings indicated that S2 subunit (heptad-repeat 1 (HR1), central helix (CH), and connector domain (CD) domains) showed low variability, low fluctuations in MD, and displayed a trimer cavity. By contrast, the receptor binding domain (RBD) domain, which is typically targeted in drug discovery programs, exhibits more sequence variability and flexibility. Interpretations from MD simulations suggest that the monomer form of spike protein is in constant motion showing transitions between an “up” and “down” state. In addition, the trimer cavity may function as a “bouncing spring” that may facilitate the homotrimer spike protein interactions with the ACE2 receptor. The feasibility of the trimer cavity as a potential drug target was examined by structure based virtual screening. Several hits were identified that have already been validated or suggested to inhibit the SARS-CoV-2 virus in published cell models. In particular, the data suggest an action mechanism for molecules including Chitosan and macrolides such as the mTOR (mammalian target of Rapamycin) pathway inhibitor Rapamycin. These findings identify a novel small molecule binding-site formed by the spike protein oligomer, that might assist in future drug discovery programs aimed at targeting the coronavirus (CoV) family of viruses.
NADPH Oxidase Gene Polymorphism is Associated with Mortality and Cardiovascular Events in 7-Year Follow-Up
Authors: Racis Milena , Sobiczewski Wojciech , Stanisławska-Sachadyn Anna , Wirtwein Marcin , Bluj Elżbieta , Nedoszytko Michał , Borzyszkowska Joanna , Limon Janusz , Rynkiewicz Andrzej , Gruchała Marcin
Published: May 2020

The CYBA gene encodes the regulatory subunit of NADPH oxidase, which maintains the redox state within cells and in the blood vessels. That led us to investigate the course of coronary artery disease (CAD) with regards to CYBA polymorphisms. Thus, we recruited 1197 subjects with coronary atherosclerosis and observed them during 7-year follow-up. Three CYBA polymorphisms: c.214C>T (rs4673), c.-932G>A (rs9932581), and c.*24G>A (1049255) were studied for an association with death, major adverse cardiovascular events (MACE) and an elective percutaneous coronary intervention or coronary artery bypass grafting (PCI/CABG). We found an association between the CYBA c.214C>T polymorphism and two end points: death and PCI/CABG. CYBA c.214TT genotype was associated with a lower risk of death than C allele (9.5% vs. 21%, p < 0.05) and a higher risk of PCI/CABG than C allele (69.3% vs. 51.7%, p < 0.01). This suggests that the CYBA c.214TT genotype may be a protective factor against death OR = 0.47 (95%CI 0.28–0.82; p < 0.01), while also being a risk factor for an elective PCI/CABG OR = 2.36 (95%CI 1.15–4.82; p < 0.05). Thus, we hypothesize that among patients with coronary atherosclerosis, the CYBA c.214TT genotype contributes to atherosclerotic plaque stability by altering the course of CAD towards chronic coronary syndrome, thereby lowering the incidence of fatal CAD-related events.
Autologous Matrix of Platelet-Rich Fibrin in Wound Care Settings: A Systematic Review of Randomized Clinical Trials
Authors: de Carvalho Chayane Karla Lucena , Fernandes Beatriz Luci , de Souza Mauren Abreu
Published: May 2020

Platelet-rich fibrin (PRF) consists of a matrix that provides the necessary elements for wound healing, acting as a biodegradable scaffold for cell migration, proliferation, and differentiation, in addition to the delivery of growth factors and angiogenesis. This study aims to determine the effectiveness of the autologous PRF in the treatment of wounds of different etiologies. We carried out a systematic review of randomized clinical trials, guided by the recommendations of the Cochrane Collaboration using the following databases: Pubmed/MEDLINE, EMBASE, Web of Science, and CENTRAL. The search strategy resulted in the inclusion of ten studies that evaluated the use of PRF dressings for the healing of acute or chronic wounds of multiple etiologies. Among the 172 participants treated with PRF in wounds of varying etiologies and different segment times, 130 presented favorable events with the use of the intervention. Among the 10 studies included, only two of them did not demonstrate better results than the control group. The studies showed clinical heterogeneity, making it impossible to perform a meta-analysis. The findings do not provide enough evidence to support the routine use of PRF dressings as the first line of treatment for the healing of acute or chronic wounds of different etiologies. There was great variability in the application of the various protocols and the ways to prepare the PRF, resulting in clinical heterogeneity. Therefore, it makes it impossible to synthesize and to collect evidence from different types of studies in the meta-analysis, which affects the results and their proper discussion.
Self-Excited Torsional Vibration in the Flexible Coupling of a Marine Propulsion Shafting System Employing Cardan Shafts
Authors: Song Myeong-Ho , Nam Taek-Kun , Lee Jae-ung
Published: May 2020

The reliability of propulsion shafting systems is a major concern for ocean-going vessels because mid-ocean repairs can be time-consuming and spare parts must be available. To address this concern, vibration modeling and experimental measurements were conducted on a propulsion shafting system with a Z-drive propeller, with the objective of identifying the source of failure for the flexible rubber coupling connecting the diesel engine with the intermediate shaft. The torsional fluctuations in the flexible coupling dramatically increased and then abruptly ceased. The modeling results revealed that the frictional losses during power transmission through the universal joints could act as an excitation force for self-excited vibration. The coupling connected to the intermediate shaft did not have sufficient radial flexibility to dampen these vibrations. To avoid the effects of the self-excited torsional vibration, it is recommended that this coupling is replaced with one that is capable of absorbing the radial shaft displacement.
Local Enhancements of the Mean Drift Wave Force on a Vertical Column Shielded by an Exterior Thin Porous Shell
Authors: Cong Peiwen , Liu Yingyi
Published: May 2020

The wave interaction with a vertical column shielded by an exterior porous shell is studied within the framework of potential flow theory. The structures are fixed rigidly at the sea bottom. The interior cylinder is impermeable, and the exterior shell is slightly porous and thin. Additionally, the exterior shell is assumed to have fine pores, and a linear pressure drop is adopted at the porous geometry. The mean drift wave force on the system is thereby formulated by two alternative ways, based respectively on the direct pressure integration, i.e., the near-field formulation, and the application of the momentum conservation theorem in the fluid domain, i.e., the far-field formulation. The consistency of the two formulations in calculating the mean drift wave force is assessed for the present problem. Numerical results illustrate that the existence of the porous shell can substantially reduce the mean drift wave force on the interior column. It also appears that the far-field formulation consists of a conventional part as well as an additional part caused by the energy dissipation through the porous geometry. The mean drift wave force on the system is dominated by the first part, which resembles that on an impermeable body. Local enhancements of the mean drift wave force are found at some specific wave frequencies at which certain propagation modes of the fluid satisfy a no-flow condition at the porous shell.
Approaching Software Engineering for Marine Sciences: A Single Development Process for Multiple End-User Applications
Authors: Magaña Pedro , Del-Rosal-Salido Juan , Cobos Manuel , Lira-Loarca Andrea , Ortega-Sánchez Miguel
Published: May 2020

Research software is currently used by a large number of scientists on a daily basis, and everything indicates that this trend will continue to increase in the future. Most of this scientific software is very often developed by the researchers themselves, who usually make it available to the rest of the scientific community. Although the relationship between science and software is unquestionably useful, it is not always successful. Some of the critical problems that scientists face include a lack of training in software development, a shortage of time and resources, or difficulty in effectively cooperating with other colleagues. Additional challenges arise in the context of increasingly common cross-cutting and multidisciplinary research. This often results in the developed software and code being slow, not reusable, lacks visibility and dissemination, and in the worst cases it is defective and unreliable. Therefore, a multidisciplinary framework is needed to meet the demands of both scientists and software engineers and handle the situation successfully. However, a multidisciplinary team is not always sufficient to solve this problem, and it is necessary to have links between scientists and developers: software engineers with a solid scientific background. This paper presents the approach used in the framework of the PROTOCOL project, and more particularly in the development of its applied software, in which a tool for the characterization of climate agents has been developed. The main guidelines of the development process include, among others, modularity, distributed control version, unit testing, profiling, inline documentation and the use of best practices and tools.
Understanding the Return of Genomic Sequencing Results Process: Content Review of Participant Summary Letters in the eMERGE Research Network
Authors: Lynch John , Sharp Richard , Aufox Sharon , Bland Sarah , Blout Carrie , Bowen Deborah , Buchanan Adam , Halverson Colin , Harr Margaret , Hebbring Scott , Henrikson Nora , Hoell Christin , Holm Ingrid , Jarvik Gail , Kullo Iftikhar , Kochan David , Larson Eric , Lazzeri Amanda , Leppig Kathleen , Madden Jill , Marasa Maddalena , Myers Melanie , Peterson Josh , Prows Cynthia , Kulchak Rahm Alanna , Ralston James , Milo Rasouly Hila , Scrol Aaron , Smith Maureen , Sturm Amy , Stuttgen Kelsey , Wiesner Georgia , Williams Marc , Wynn Julia , Williams Janet
Published: May 2020

A challenge in returning genomic test results to research participants is how best to communicate complex and clinically nuanced findings to participants in a manner that is scalable to the large numbers of participants enrolled. The purpose of this study was to examine the features of genetic results letters produced at each Electronic Medical Records and Genomics (eMERGE3) Network site to assess their readability and content. Letters were collected from each site, and a qualitative analysis of letter content and a quantitative analysis of readability statistics were performed. Because letters were produced independently at each eMERGE site, significant heterogeneity in readability and content was found. The content of letters varied widely from a baseline of notifying participants that results existed to more detailed information about positive or negative results, as well as materials for sharing with family members. Most letters were significantly above the Centers for Disease Control-suggested reading level for health communication. While continued effort should be applied to make letters easier to understand, the ongoing challenge of explaining complex genomic information, the implications of negative test results, and the uncertainty that comes with some types of test and result makes simplifying letter text challenging.
Increased Demodex Density in Patients Hospitalized for Worsening Heart Failure
Authors: Yüksel Serkan , Pancar Yüksel Esra
Published: May 2020

Infection is an important factor leading to the exacerbation of heart failure (HF), resulting in hospitalization. Demodex species are obligatory parasites in human skin, and increased density was reported in immunocompromised patients. In this study, we aimed to investigate the Demodex density in hospitalized HF patients compared to that of healthy controls. Methods: This study included 36 HF patients and 36 age and sex-matched healthy controls. Five standardized biopsies were taken from the face of participants and assessed for Demodex by a light microscope. Results: At least one Demodex mite was detected in 20 HF patients and nine of the control group. The number of Demodex mites was significantly higher in the HF group (median 1; min. 0 and max. 10) compared to the control group (median 0; minimum. 0 and maximum. 3). Demodicidosis was positive in 14 of the HF patients. Demodicidosis was not detected in the control group. Conclusions: This study showed that Demodex positivity is more common in HF patients hospitalized for HF exacerbation. Demodicidosis should be considered in hospitalized HF patients.
Landscape Planning for an Agricultural Research Center: A Research-by-Design Case Study in Chiang Mai, Thailand
Authors: Charoenlertthanakit Nadchawan , Wanitchayapaisit Chulalux , Yaipimol Ekachai , Surinseng Vipavee , Suppakittpaisarn Pongsakorn
Published: May 2020

Effective planning at the landscape scale is a difficult but crucial task. Modern landscape planning requires economic success, ecological resilience, and environmental justice. Thus, planners and designers must learn to use a deliberative approach in planning: an approach in which decisions are made with the common understanding of stakeholders. This notwithstanding, there is a lack of localized and site-specific design examples for deliberative planning. One of the lacking examples is agricultural research station, which is unique because it balances economic, academic, and public uses. This study used a Research-by-Design Method to explore deliberative planning for an agricultural research station in Chiang Mai, Thailand. Field surveys, interviews, and archival search were conducted for database. Design decisions were delivered via linear-combination suitability analysis. We found that the site was viewed differently by different sets of users. The basic and safety infrastructure was the top priority, and clear direction of governance was crucial to move the site forward in the future. This study was one of the first recorded attempts to design an agricultural research center via the Research-by-Design process. The method and results of the research contribute to the growing body of evidence to support the need of evidence-based design and planning for all sites.
Loss of Concentration May Occur by Blink Inhibition in DED Simulation Models
Authors: Mitsukura Yasue , Negishi Kazuno , Ayaki Masahiko , Santo Mayuko , Kawashima Motoko , Tsubota Kazuo
Published: May 2020

Purpose: Patients with dry eye disease (DED) often suffer productivity loss and distress due to bothersome symptoms. The aim of this study was to objectively quantify and compare productivity-related emotional states obtained from brain waveforms in natural and simulated DED conditions. Method: 25 healthy adults (6 females and 19 males; mean age ± standard deviation, 22.6 ± 8.3 years) were recruited for the study, which included an electroencephalogram (EEG), measurements of interblinking time, and questionnaires. DED was simulated by suppressing blinking, while spontaneous blinking served as a control. Elements of concentration, stress, and alertness were extracted from the raw EEG waveforms and the values were compared during spontaneous and suppressed blinking. The relation with DED-related parameters was then explored. Written informed consent was obtained from all participants. Results: All participants successfully completed the experimental protocol. Concentration significantly decreased during suppressed blinking in 20 participants (80%), when compared with spontaneous blinking, whereas there were no or small differences in stress or alertness between spontaneous and suppressed blinking. The change in concentration was correlated with interblinking time (β = −0.515, p = 0.011). Conclusion: Loss of concentration was successfully captured in an objective manner using the EEG. The present study may enable us to understand how concentration is affected during blink suppression, which may happen in office work, particularly during computer tasks. Further study using detailed ocular evaluation is warranted to explore the effect of different interventions.
3-(4-Bromophenyl)-4-{[3-hydroxy-6-(hydroxymethyl)-4-oxo-4H-pyran-2-yl](m-tolyl)methyl}isoxazol-5(2H)-one
Authors: Ryzhkova Yuliya E. , Ryzhkov Fedor V. , Elinson Michail N.
Published: May 2020

In this communication, the electrochemically induced multicomponent transformation of 3-methylbenzaldehyde, 3-(4-bromophenyl)isoxazol-5(4H)-one and kojic acid in n-PrOH in an undivided cell in the presence of sodium bromide was carefully investigated to give 3-(4-bromophenyl)-4-{[3-hydroxy-6-(hydroxymethyl)-4-oxo-4H-pyran-2-yl](m-tolyl)methyl}isoxa- zol-5(2H)-one in good yield. The structure of the new compound was established by means of elemental analysis, mass-, nuclear magnetic resonance and infrared spectroscopy. Furthermore, its structure was determined and confirmed by X-ray analysis. The synthesized compound is a promising compound for different biomedical applications, and, in particular, for the regulation of inflammatory diseases, as shown by docking studies in this research.
Mineral Materials Coated with and Consisting of MnOx—Characteristics and Application of Filter Media for Groundwater Treatment: A Review
Authors: Michel Magdalena M. , Reczek Lidia , Papciak Dorota , Włodarczyk-Makuła Maria , Siwiec Tadeusz , Trach Yuliia
Published: May 2020

For groundwater treatment, the technologies involving oxidation on MnOx filter bed are beneficial, common, and effectively used. The presence of MnOx is the mutual feature of filter media, both MnOx-coated mineral materials like quartz sand and gravel, chalcedonite, diatomite, glauconite, zeolite, or anthracite along with consisting of MnOx manganese ores. This review is based on the analysis of research and review papers, commercial data sheets, and standards. The paper aimed to provide new suggestions and useful information for further investigation of MnOx filter media for groundwater treatment. The presented compilations are based on the characteristics of coatings, methods, and conditions of its obtaining and type of filter media. The relationship between the properties of MnOx amendments and the obtained purification effects as well as the commonly used commercial products, their features, and applications have been discussed. The paper concludes by mentioning about improving catalytic/adsorption properties of non-reactive siliceous media opposed to ion-exchange minerals and about possible significance of birnessite type manganese oxide for water treatment. Research needs related to the assessment of the use MnOx filter media to heavy metals removal from groundwater in field operations and to standardize methodology of testing MnOx filter media for water treatment were identified.
Application of Dynamic Beam Positioning for Creating Specified Structures and Properties of Welded Joints in Electron-Beam Welding
Authors: Olshanskaya Tatyana , Belenkiy Vladimir , Fedoseeva Elena , Koleva Elena , Trushnikov Dmitriy
Published: May 2020

The application of electron beam sweep makes it possible to carry out multifocal and multi-beam welding, as well as combine the welding process with local heating or subsequent heat treatment, which is important when preparing products from thermally-hardened materials. This paper presents a method of electron beam welding (EBW) with dynamic beam positioning and its experimental-calculation results regarding the formation of structures and properties of heat-resistant steel welded joints (grade of steel 20Cr3MoWV). The application of electron beam oscillations in welding makes it possible to change the shape and dimensions of welding pool. It also affects the crystallization and formation of a primary structure. It has been established that EBW with dynamic beam positioning increases the weld metal residence time and the thermal effect zone above the critical A3 point, increases cooling time and considerably reduces instantaneous cooling rates as compared to welding without beam sweep. Also, the difference between cooling rates in the depth of a welded joint considerably reduces the degree of structural non-uniformity. A bainitic–martensitic structure is formed in the weld metal and the thermal effect zone throughout the whole depth of fusion. As a result of this structure, the level of mechanical properties of a welded joint produced from EBW with dynamic electron beam positioning approaches that of parent metal to a greater extent than in the case of welding by a static beam. As a consequence, welding of heat-resistant steels reduces the degree of non-uniformity of mechanical properties in the depth of welded joints, as well as decreases the level of hardening of a welded joint in relation to parent metal.
Volumetric Changes in the Upper Airways after Rapid and Slow Maxillary Expansion in Growing Patients: A Case-Control Study
Authors: Lanteri Valentina , Farronato Marco , Ugolini Alessandro , Cossellu Gianguido , Gaffuri Francesca , Parisi Francesca Maria Rita , Cavagnetto Davide , Abate Andrea , Maspero Cinzia
Published: May 2020

The aim is to evaluate changes in the volume of the upper airways before and after slow maxillary expansion (SME) obtained with the flexible properties of a nickel titanium leaf spring and rapid maxillary expansion (RME) with a conventional Hyrax appliance in growing patients. The records of 1200 orthodontic patients undergoing maxillary expansion from 2018 to 2019 were analyzed; among these pre and post treatment CBCT scans of 22 patients (mean age 8.2 ± 0.6 years old) treated by SME were compared with those obtained from 22 patients (mean age 8.1 ± 0.7 years old) treated by RME banded on the second primary molars. The following inclusion criteria were used: Maxillary transverse constriction, good general health, and no previous orthodontic treatment. Volumes of nasal cavity (NCavV), nasopharynx (NsPxV), and right and left maxillary sinuses (MSV) were calculated with ITK-SNAP. Shapiro–Wilk test revealed a normal distribution of data in each group. Paired t-test was used for within-group comparisons and independent t-test for between-group comparisons. Statistically significant increases occurred in NCavV, NsPxV, and MSVs after treatment with both appliances. No statistically significant difference between the appliances occurred in NCavV, NsPxV, and MSVs. Method error was considered negligible (mean intra-operator and inter-operator intraclass correlation coefficient were 0.928 and 0.911, respectively). It appears that both appliances produce similar effects on the different segments of the upper airway tract.