bluuebunny/crossref_metadata_2025 huggingface dataset:
Phosphoserine phosphatase as an indicator for survival through potentially influencing the infiltration levels of immune cells in neuroblastoma
Authors: Zeng Liang , Liu Xiao-Yun , Chen Kai , Qin Liang-Jun , Wang Feng-Hua , Miao Lei , Li Le , Wang Hai-Yun
Published: August 2022

Introduction: Metabolic deregulation, a hallmark of cancer, fuels cancer cell growth and metastasis. Phosphoserine phosphatase (PSPH), an enzyme of the serine metabolism pathway, has been shown to affect patients’ prognosis in many cancers but its significance in neuroblastoma remains unknown. Here, we show that the functional role and potential mechanism of PSPH and it is correlated with survival of neuroblastoma patients.

Patients and Methods: The TARGET dataset (n = 151) and our hospital-based cases (n = 55) were used for assessing the expression level of PSPH associated with survival in neuroblastoma patients, respectively. Then, in vitro experiments were performed to define the role of PSPH in neuroblastoma. The ESTIMATE and TIMER algorithms were utilized to examine the correlation between PSPH expression level and abundance of immune cells. Further, Kaplan-Meier survival analysis was performed to evaluate the effect of both PSPH and immune cells on patients’ prognosis.

Results: High expression of PSPH was significantly associated with unfavorable overall survival (OS) and event-free survival (EFS) in both the TARGET dataset and our hospital-based cases, and was an independent predictor of OS (hazard ratio, 2.00; 95% confidence intervals, 1.21–3.30, p = 0.0067). In vitro experiments showed that high expression of PSPH significantly promoted cell growth and metastasis. Further, the ESTIMATE result suggested that high expression level of PSPH was negatively associated with low stromal and ESTIMATE score. Specifically, high PSPH expression was found to be negatively associated with CD8+ T cell, macrophages and neutrophils, which negatively affected survival of neuroblastoma patients (p < 0.0001, p = 0.0005, and p = 0.0004, respectively).

Conclusion: These findings suggested that PSPH expression could be a promising indicator for prognosis and immunotherapy in neuroblastoma patients by potentially influencing infiltration levels of immune cells.
The Potential of Small Molecules to Modulate the Mitochondria–Endoplasmic Reticulum Interplay in Alzheimer’s Disease
Authors: Dentoni Giacomo , Castro-Aldrete Laura , Naia Luana , Ankarcrona Maria
Published: August 2022

Alzheimer’s disease (AD) is the most common neurodegenerative disease affecting a growing number of elderly individuals. No disease-modifying drugs have yet been identified despite over 30 years of research on the topic, showing the need for further research on this multifactorial disease. In addition to the accumulation of amyloid β-peptide (Aβ) and hyperphosphorylated tau (p-tau), several other alterations have been associated with AD such as calcium (Ca2+) signaling, glucose-, fatty acid-, cholesterol-, and phospholipid metabolism, inflammation, and mitochondrial dysfunction. Interestingly, all these processes have been associated with the mitochondria–endoplasmic reticulum (ER) contact site (MERCS) signaling hub. We and others have hypothesized that the dysregulated MERCS function may be one of the main pathogenic pathways driving AD pathology. Due to the variety of biological processes overseen at the MERCS, we believe that they constitute unique therapeutic targets to boost the neuronal function and recover neuronal homeostasis. Thus, developing molecules with the capacity to correct and/or modulate the MERCS interplay can unleash unique therapeutic opportunities for AD. The potential pharmacological intervention using MERCS modulators in different models of AD is currently under investigation. Here, we survey small molecules with the potential to modulate MERCS structures and functions and restore neuronal homeostasis in AD. We will focus on recently reported examples and provide an overview of the current challenges and future perspectives to develop MERCS modulators in the context of translational research.
Lactylation, an emerging hallmark of metabolic reprogramming: Current progress and open challenges
Authors: Liu Xuelian , Zhang Yu , Li Wei , Zhou Xin
Published: August 2022

Lactate, the end product of glycolysis, efficiently functions as the carbon source, signaling molecules and immune regulators. Lactylation, being regulated by lactate, has recently been confirmed as a novel contributor to epigenetic landscape, not only opening a new era for in-depth exploration of lactate metabolism but also offering key breakpoints for further functional and mechanistic research. Several studies have identified the pivotal role of protein lactylation in cell fate determination, embryonic development, inflammation, cancer, and neuropsychiatric disorders. This review summarized recent advances with respect to the discovery, the derivation, the cross-species landscape, and the diverse functions of lactylation. Further, we thoroughly discussed the discrepancies and limitations in available studies, providing optimal perspectives for future research.
Ultrafast plasma method allows rapid immobilization of monatomic copper on carboxyl-deficient g-C3N4 for efficient photocatalytic hydrogen production
Authors: Xu Shuchang , Zhang Zhihao , Wang Daqian , Lu Junyang , Guo Ying , Kang Shifei , Chang Xijiang
Published: August 2022

Transition-metal monometallic photocatalysts have received extensive attention owing to the maximization of atomic utilization efficiency. However, in previous related works, single-atom loading and stability are generally low due to limited anchor sites and mechanisms. Recently, adding transition-metal monatomic sites to defective carbon nitrides has a good prospect, but there is still lack of diversity in defect structures and preparation techniques. Here, a strategy for preparing defect-type carbon-nitride–coupled monatomic copper catalysts by an ultrafast plasma method is reported. In this method, oxalic acid and commercial copper salt are used as a carboxyl defect additive and a copper source, respectively. Carbon nitride samples containing carboxyl defects and monatomic copper can be processed within 10 min by one-step argon plasma treatment. Infrared spectroscopy and nuclear magnetic resonance prove the existence of carboxyl defects. Spherical aberration electron microscopy and synchrotron radiation analysis confirm the existence of monatomic copper. The proportion of monatomic copper is relatively high, and the purity is high and very uniform. The Cu PCN as-prepared shows not only high photo-Fenton pollutant degradation ability but also high photocatalytic hydrogen evolution ability under visible light. In the photocatalytic reaction, the reversible change of Cu+/Cu2+ greatly promotes the separation and transmission of photogenerated carriers and improves the utilization of photoelectrons. The photocatalytic hydrogen evolution rate of the optimized sample is 8.34 mmol g−1·h−1, which is 4.54 times that of the raw carbon nitride photocatalyst. The cyclic photo-Fenton experiment confirms the catalyst has excellent repeatability in a strong oxidation environment. The synergistic mechanism of the photocatalyst obtained by this plasma is the coordination of single-atom copper sites and carboxyl defect sites. The single copper atoms incorporated can act as an electron-rich active center, enhancing the h+ adsorption and reduction capacity of Cu PCN. At the same time, the carboxyl defect sites can form hydrogen bonds to stabilize the production of hydrogen atoms and subsequently convert them to hydrogen because of the unstable hydrogen bond structure. This plasma strategy is green, convenient, environment-friendly, and waste-free. More importantly, it has the potential for large-scale production, which brings a new way for the general preparation of high-quality monatomic catalysts.
Comprehensive review of α-carboline alkaloids: Natural products, updated synthesis, and biological activities
Authors: Li Deping , Yang Renze , Wu Jun , Zhong Bin , Li Yan
Published: August 2022

α-carboline (9H-pyrido[2,3-b]indole), contains a pyridine ring fused with an indole backbone, is a promising scaffold for medicinal chemistry. In recent decades, accumulating evidence shows that α-carboline natural products and their derivatives possess diverse bioactivities. However, hitherto, there is no comprehensive review to systematically summarize this important class of alkaloids. In this perspective, this paper represents the first review to provide a comprehensive description of α-carbolines including natural products, updated literature of synthesis, and their diverse biological activities. Their biological activities including antitumor, anti-microbial, anti-Alzheimer’s disease, anti-atherosclerosis, and antioxidant activities were hilighted. And the targets and the main structure activity relationships (SARs) will be presented. Finally, challenges and future directions of this class of compounds will be discussed. This review will be helpful in understanding and encouraging further exploration for this group of alkaloids.
2-(1,2,4-triazole-5-yl)-1,3,4-oxadiazole as a novel building block for energetic materials
Authors: Dong Zheting , Wu Zhengqiang , Zhang Qiang , Xu Yuangang , Lu Guo-Ping
Published: August 2022

The exploration of novel nitrogen-rich heterocyclic building blocks is of importance in the field of energetic materials. A series of 2-(1,2,4-triazole-5-yl)-1,3,4-oxadiazole derivatives based on a new energetic skeleton have been first synthesized by a simple synthetic strategy. All three compounds are well-characterized by IR spectroscopy, NMR spectroscopy and thermal analysis. The compounds 5 and 8 are further characterized by single-crystal X-ray diffraction analysis. 8 and its salts (8a-8c) possess relative high decomposition temperature and low sensitivity, while 5 exhibits low decomposition temperature and high sensitivity. According to EXPLO5 calculation results of detonation performance, both 5 and 8 display acceptable detonation velocities (D) of 8450 m/s and 8130 m/s and detonation pressures (P) of 31.6 GPa and 29.2 GPa, respectively. Furthermore, 5 containing a rare diazonium ylide structure shows high impact sensitivity (4.5 J), making it has a potential as a primary explosive.
Tribbles 3 deficiency promotes atherosclerotic fibrous cap thickening and macrophage-mediated extracellular matrix remodelling
Authors: Martinez-Campesino Laura , Kocsy Klaudia , Cañedo Jaime , Johnston Jessica M. , Moss Charlotte E. , Johnston Simon A. , Hamby Stephen , Goodall Alison H. , Redgrave Jessica , Francis Sheila E. , Kiss-Toth Endre , Wilson Heather L.
Published: August 2022

Tribbles 3 (TRIB3) modulates lipid and glucose metabolism, macrophage lipid uptake, with a gain-of-function variant associated with increased cardiovascular risk. Here we set out to examine the role of this pseudokinase in atherosclerotic plaque development. Human endarterectomy atherosclerotic tissue specimens analysed by immunofluorescence showed upregulated TRIB3 in unstable plaques and an enrichment in unstable regions of stable plaques. Atherosclerosis was induced in full body Trib3KO and Trib3WT littermate mice by injecting mPCSK9 expressing adeno-associated virus and western diet feeding for 12 weeks. Trib3KO mice showed expanded visceral adipose depot while circulatory lipid levels remained unaltered compared to wildtype mice. Trib3KO mice aortae showed a reduced plaque development and improved plaque stability, with increased fibrous cap thickness and collagen content, which was accompanied by increased macrophage content. Analysis of both mouse and human macrophages with reduced TRIB3 expression showed elongated morphology, increased actin expression and altered regulation of genes involved in extracellular matrix remodelling. In summary, TRIB3 controls plaque development and may be atherogenic in vivo. Loss of TRIB3 increases fibrous cap thickness via altered metalloproteinase expression in macrophages, thus inhibiting collagen and elastic fibre degradation, suggesting a role for TRIB3 in the formation of unstable plaques.
The roles and mechanisms of epigenetic regulation in pathological myocardial remodeling
Authors: Zhao Kun , Mao Yukang , Li Yansong , Yang Chuanxi , Wang Kai , Zhang Jing
Published: August 2022

Pathological myocardial remodeling was still one of the leading causes of death worldwide with an unmet therapeutic need. A growing number of researchers have addressed the role of epigenome changes in cardiovascular diseases, paving the way for the clinical application of novel cardiovascular-related epigenetic targets in the future. In this review, we summarized the emerged advances of epigenetic regulation, including DNA methylation, Histone posttranslational modification, Adenosine disodium triphosphate (ATP)-dependent chromatin remodeling, Non-coding RNA, and RNA modification, in pathological myocardial remodeling. Also, we provided an overview of the mechanisms that potentially involve the participation of these epigenetic regulation.
Metabolic reprogramming: A novel metabolic model for pulmonary hypertension
Authors: Liu Xuhan , Zhang Liping , Zhang Weihua
Published: August 2022

Pulmonary arterial hypertension, or PAH, is a condition that is characterized by pulmonary artery pressures above 20 mmHg (at rest). In the treatment of PAH, the pulmonary vascular system is regulated to ensure a diastolic and contraction balance; nevertheless, this treatment does not prevent or reverse pulmonary vascular remodeling and still causes pulmonary hypertension to progress. According to Warburg, the link between metabolism and proliferation in PAH is similar to that of cancer, with a common aerobic glycolytic phenotype. By activating HIF, aerobic glycolysis is enhanced and cell proliferation is triggered. Aside from glutamine metabolism, the Randle cycle is also present in PAH. Enhanced glutamine metabolism replenishes carbon intermediates used by glycolysis and provides energy to over-proliferating and anti-apoptotic pulmonary vascular cells. By activating the Randle cycle, aerobic oxidation is enhanced, ATP is increased, and myocardial injury is reduced. PAH is predisposed by epigenetic dysregulation of DNA methylation, histone acetylation, and microRNA. This article discusses the abnormal metabolism of PAH and how metabolic therapy can be used to combat remodeling.
Natural Gas Geochemistry of Jurassic Shaximiao Formation in the Western Sichuan Basin, China: Fault-Controlled Differentiation in Accumulation Process
Authors: Dong Jun , Li Shubing , Ye SuJuan , Li Qing , Wu Changrong
Published: August 2022

The Jurassic Shaximiao Formation in western Sichuan has made significant contributions to the natural gas reserves and production of China, with many gas fields successively discovered. The accumulation evolution progress and discrepant gathering law are continuously improved with the constantly deepened exploration and development. In order to find evidence for the difference in gas accumulation and the method to reshape the migration and accumulation of natural gas and their dynamic processes based on time and space, this study analyzes the dynamic accumulation process of the Middle Jurassic Shaximiao reservoir in the Zhongjiang gas field using the geochemical parameters, including the conventional composition of natural gas, light hydrocarbon components, carbon isotope, and formation water, of more than 160 natural gas samples from 155 wells. The results show that the natural gas-filling period, migration patterns, and phase state, etc., of the Shaximiao Formation are closely related to the distance between the hydrocarbon source faults. The geochemistry tracer parameters of natural gas and formation water reveal abundant information about the accumulation process. The Sha-3 gas reservoir, featuring a two-period hydrocarbon supply, has two migration patterns, i.e., seepage and diffusion, dominated by the water-soluble phase. Similarly, the Sha-2 gas reservoir, featuring a late single hydrocarbon supply, has the same migration patterns, dominated by the miscible phase of the water-soluble phase and free phase. The main migration pattern of the Sha-1 reservoir, which features a late single hydrocarbon supply, is seepage, dominated by the free phase. From the lower part of the Sand-3 gas reservoir to the upper part of the Sha-1 gas reservoir, the natural gas exhibits a trend where the early–late continuous filling turns into the late single filling, the lateral migration distance of oil and gas becomes shorter gradually, and the gas-rich range of the gas reservoir decreases gradually. The results of this study can provide a valuable reference for gas reservoirs controlled by faults and river sand bodies.
Overlying rock movement and mining pressure in a fully mechanized caving face with a large dip angle
Authors: Chen Ying , Wang Zhiwen , Hui Qianjia , Zhu Zhijie , Sun Dequan , Chen Yang , Zhang Xiufeng , Wang Zhaoyi , Wang Jian , Zhao Jian
Published: August 2022

The overlying rock movement and mining pressure in the fully mechanized caving face with a large dip angle were systematically investigated according to theoretical analysis, similar material simulation, numerical calculation, and field monitoring. The following conclusions were obtained: 1) the theoretical analysis showed that the roof movement characteristics at different positions of the working face are quite different. The mining pressure in the upper section of the working face is primarily controlled by the structural instability of the main roof. The stable structure of the main roof is easily formed in the middle and lower sections of the working face, and the mining pressure is mostly controlled by the collapse of the immediate roof. 2) The results of similar material simulations indicated that the height of the fracture zone in different areas of the working face is different due to the influence of a large dip angle. The height of the fracture zone formed in the upper section of the working face is significantly larger than that formed in the lower section of the working face. 3) The numerical calculation suggested that the residual coal pillar of the overlying coal seam has a certain influence on the mining pressure of the 9-301 working face, making the advanced abutment pressure in the range of 80 m close to the main gate under the coal pillar more obvious. 4) The field pressure monitoring results demonstrated that the influence range of the advanced abutment pressure close to the upper part of the working face is greater than that close to the lower part of the working face, and the peak point is closer to the mining rib. Affected by the overlying residual coal pillar, the hydraulic support resistance and the peak value of advanced abutment pressure in the lower part of the working face are greater than those in the upper part. Both the initial and periodic pressure intervals of the upper section of the working face are smaller than those of the lower section. The results of this research will provide a scientific basis for the reasonable determination of the control measures for the mining pressure.
Analogical model tests on repeated surficial failure of dry granular slopes confined by retaining walls
Authors: Yang Hufeng , Xing Bencong , Jiang Hu , Cheng Qiangong
Published: August 2022

In alpine and gorge regions, surficial failure of granular slopes has seriously affected infrastructure construction and the safe operation of transportation networks. This research focuses on dry granular slopes confined by retaining walls. Repeated surficial failure of granular slopes has been explored through indoor physical model tests. The results show that surficial failure presents low frequency and large scale for granular slopes dominated by coarse particles. The mass of debris that crosses retaining walls indicates activity level of granular slopes. When this mass for each surficial failure gradually decreases, it is highly likely that large-scale surficial failure will occur. As the amount of debris deposited on the slope increases, it is probable that the debris mass falling during a massive sliding event will be many times that of the previous sliding event. In engineering practice, it is necessary during road cleaning processes to record the volume of debris accumulated on the road. A continuous increase in the slope angle indicates a high probability of large-scale surficial failure. For each granular slope with a unique particle composition, there is a critical change rate for early warning of surficial failure, which can be determined by long-term monitoring.
Evidence-based education: Objections and future directions
Authors: Dekker Izaak , Meeter Martijn
Published: August 2022

Over the past two decades, educational policymakers in many countries have favored evidence-based educational programs and interventions. However, evidence-based education (EBE) has met with growing resistance from educational researchers. This article analyzes the objections against EBE and its preference for randomized controlled trials (RCTs). We conclude that the objections call for adjustments but do not justify abandoning EBE. Three future directions could make education more evidence-based whilst taking the objections against EBE into account: (1) study local factors, mechanisms, and implementation fidelity in RCTs, (2) utilize and improve the available longitudinal performance data, and (3) use integrated interventions and outcome measures.
Effects of liraglutide or lifestyle interventions combined with other antidiabetic drugs on abdominal fat distribution in people with obesity and type 2 diabetes mellitus evaluated by the energy spectrum ct: A prospective randomized controlled study
Authors: Yu Dongni , Zou Mingzhu , Pan Qi , Song Yan , Li Miao , Zhang Xianbo , Zhou Yan , Wang Xiaoxia , Guo Lixin
Published: August 2022

Objective

To study the effects of liraglutide or lifestyle interventions combined with other antidiabetic drugs on glucose metabolism and abdominal fat distribution in patients with obesity and type 2 diabetes mellitus (T2DM).

Methods

From April 30, 2020, to April 30, 2022, a prospective randomized controlled study was carried out at the Endocrinology Department of Beijing Hospital, the National Center of Gerontology. According to the in- and exclusion criteria and by the random table method, revisited T2DM patients were selected as the research subjects and were allocated into a Study group (taking liraglutide) and a Control group (underwent lifestyle interventions). All patients received continuous 12-weeks interventions to the endpoint, and the changes of value [Δ=(endpoint)-(baseline)] of physical measurements, blood tests, the energy spectrum CT examination results, and body composition analysis results were analyzed and compared.

Results

A total of 85 people completed this study, and among them, 47 were in the Study group and 38 were in the Control group. Compared with the Control group, the changes of hemoglobin A1c (HbA1c) level (-0.78 ± 1.03% vs. -1.57 ± 2.00%, P=0.025), visceral fat area (0.91 ± 16.59 cm2 vs. -7.1 ± 10.17 cm2, P=0.011), and subcutaneous fat area of abdomen [0 (-18.75, 15.5) cm2 vs. -16.5 (-41.75, -2.25) cm2, P=0.014] were all greater in the Study group. The adverse events caused by liraglutide were mainly concentrated in the gastrointestinal system and all of them were minor adverse events.

Conclusion

Liraglutide can be the drug of choice for weight management and reduction of abdominal fat distribution in patients with obesity and T2DM.
Medications and medical costs for diabetes patients with or without chronic respiratory disease in Beijing, China: A retrospective study
Authors: Qiao Jingtao , Tan Zheng , Xu Xiaomao , Zhou Yan , Wang Weihao , Luo Jingyi , Fan Jingwen , Pan Qi , Guo Lixin
Published: August 2022

Aims

The cost of drug regimens prescribed to Chinese patients has not been evaluated. This study aims to evaluate the medical costs and hypoglycemic agents for diabetes mellitus patients with or without chronic respiratory disease in Beijing, and to investigate the changes in the costs and number of antidiabetic medications used for diabetes patients with chronic respiratory disease from 2016 to 2018.

Methods

This observational, retrospective study included diabetes patients with outpatient medication records from Beijing Medical Insurance between 2016 and 2018. The medications, including hypoglycemic and nonhypoglycemic drugs, insulin dosage, comorbidities, diabetes-related complications, treatment strategies, and annual medical costs, were recorded.

Results

This study included 2,853,036 diabetes patients from 2016 to 2018. About 18.95%–20.53% of patients with chronic respiratory disease were predominantly distributed among those aged 45–84 years (88.7%–89.1%). Diabetes patients with chronic respiratory disease used more medications (4.48 ± 2.41 vs. 3.76 ± 2.33) and had higher total annual drug costs (¥12,286 ± 10,385 vs. ¥9700 ± 9202) to treat more comorbidities (2.52 ± 1.53 vs. 2.05 ± 1.85) than those without chronic respiratory disease (p <.0001, respectively). From 2016 to 2018, diabetes patients with chronic respiratory disease had a 4.2% increase in medication, a 1.9% decrease in comorbidities, and a 5.4% decrease in total annual drug costs.

Conclusions

In summary, diabetes patients with chronic respiratory disease had more comorbidities, required more hypoglycemic drugs, and had higher medical costs. During 2016–2018, diabetes patients with chronic respiratory disease used more medications and spent less money on medical care.
MITO: A new operational model for the forecasting of the Mediterranean sea circulation
Authors: Napolitano E. , Iacono R. , Palma M. , Sannino G. , Carillo A. , Lombardi E. , Pisacane G. , Struglia M. V.
Published: August 2022

Availability of detailed short-term forecasts of the ocean main characteristics (circulation and waves) is essential for a correct management of the human activities insisting on coastal areas. These activities include the extraction of renewable energy, which has developed in recent years, and will play an important role in the context of future blue growth. The present work describes the implementation of a new ocean operational system, named MITO, that provides daily 5 days forecasts of the Mediterranean Sea circulation. Distinctive features of this system are the inclusion of the main effects of the tidal forcing, both local and propagating from the Atlantic, and the high spatial detail. The horizontal resolution is of 1/48° (about 2 km) in most of the computational domain, and is smoothly increased (down to few hundred meters) in key passages, such as the Gibraltar Strait and the Turkish Straits, to correctly resolve the complex local dynamics. Initial and boundary conditions for MITO are taken from the reference European operation model of Copernicus, which covers the Mediterranean Sea with a uniform resolution of 1/24°. A thorough validation of the new system is performed, analyzing the forecasts of the year 2020, whose results are compared with in situ and remote observational data (sea surface temperature, altimeter data, temperature and salinity profiles by floats, tide-gauge measurements, available through the Copernicus portal) using the same large-scale metrics applied in the validation of the Copernicus operational model. MITO results are generally found in very good agreement with the observations, despite the fact that the model does not make explicit use of data assimilation. We also give examples of the capability of the model to correctly describe complex local mesoscale dynamics, and point out aspects that need to be improved, which will be addressed in a future upgrade of the operational implementation.
Safe utilization of cultivated land in high-risk areas of soil heavy metal pollution based on soil resilience
Authors: Song Wen , Zhang Huizhong , Li Xinju , Song Hengfei , Niu Beibei , Shi Xianjun , Li Junying
Published: August 2022

With global urbanization and industrialization, environmental pollution and food safety problems caused by soil heavy metal pollution occur frequently. To realize the safe utilization of cultivated land resources in high-risk areas of heavy metal pollution, we present an approach to safe utilization classification and management in this study. A typical agricultural area around industrial and mining enterprises located in the economic belt of the Yangtze River Delta was chosen as the research area with cultivated soil as the research object. A total of 1,139 geochemical survey sampling sites and soil survey data were used for this research. Initially, the potential ecological risk index was used to assess the potential ecological risk of heavy metals in cultivated soil, and key soil physical and chemical indicators were chosen to assess soil resilience. Next, the safe utilization classification of cultivated land was carried out by combining the potential ecological risk of soil heavy metals with soil resilience. Then, the specific classification management strategy was developed according to ecological risk factor types and crop types. The results showed that the production and operation activities of industrial and mining enterprises in the study area contributed significantly to soil Cd and Hg pollution, and the potential ecological risk of heavy metals in the surrounding soil was high. The soil resilience of cultivated land in the study area was generally not high. The clay content and cation exchange capacity (CEC) were the main strong restrictive indicators, while CaO, soil organic carbon (SOC), and pH were the main medium restrictive indicators. Natural conditions such as topography, hydrogeology, and soil-forming parent material differed by region, resulting in different restrictive factors. There were 16 safe utilization types in the study area. Different management strategies were proposed based on various soil potential ecological risks, soil resilience, ecological risk factor types, and crop types. This study can offer fresh perspectives on the safe utilization classification and management of land resources in high-risk areas of soil pollution and serve as a reference for sustainable intensification.
Do Innovation in Environmental-Related Technologies and Renewable Energies Mitigate the Transport-Based CO2 Emissions in Turkey?
Authors: Alnour Mohammed
Published: August 2022

Given the unprecedented level of air pollution in urban areas, green transport systems has been a subject to an important debate in academic and policymaking circles. Despite the considerable outputs of the attendant literature, most of empirical studies to date have relied on conventional econometric models in which structural shocks are not controlled. This study, therefore, aims to offer a new perceptive of the dynamic connection between renewable energy, environment-related technological innovation, and transport-based CO2 emissions in Turkey during 1990Q1 to 2014Q1 by applying the Structural Vector Autoregressive approach (SVAR). Furthermore, to explore the co-movements and the lead-lag interrelations among the study variables, the wavelet coherence technique was used. The wavelet coherence technique circumvents the other traditional causality approaches by detecting the causal interrelation between the underlying series at different frequencies. The findings disclose that environment-related technological innovation has no reliable power to explain the variation in CO2 emissions from the transport sector. Solar energy is found to impact the CO2 emissions positively in the long run, while biofuels hold the same effect in short run. Moreover, per capita GDP and urbanization significantly impact the carbon emissions from the transport system in the long run with a negative sign. The wavelet analysis reveals that renewables and environmental-related technological innovation lead the transport-based CO2 emissions. The fourth and 16th periods are the most dominant frequencies. Accordingly, the study suggests that innovation in environment-related technologies is not enough to mitigate the pollution that stemming from the transport system in Turkey, it should be accompanied by strong and effective environmental measures. These policies might include environmental taxations, carbon pricing and trading schemes, which aim not only to prevent the pollution and over-extraction of resources, but also to promote the public revenues from different activities that related to environmental purposes and other applications such as energy product and vehicle fuels. In addition, it is suggested to strengthening the transportation system through the deployment of renewables and high-tech eco-friendly modes of transportation.
Insights into the anaerobic digestion of fecal sludge and food waste in Tanzania
Authors: Kilucha Malimi , Cheng Shikun , Minza Selele , Nasiruddin Sayed Mohammad , Velempini Kgosietsile , Li Xinying , Wang Xuemei , Mokeira Doroth Kerage , Li Zifu
Published: August 2022

With the increasing demand for renewable energy and environmental protection, biogas technology has attracted considerable attention around the world. Fecal sludge (FS) is rich in organic matter, and it contains high concentrations of excreted pathogens that cause gastro-intestinal infection. In Tanzania, fecal sludge management from on-site sanitation systems poses a threat on environmental safety. This study aimed to assess the feasibility of the use of anaerobic digestion (AD) for the treatment of FS and the production of biogas as renewable energy to achieve multiple benefits in Tanzania. For the experiments, FS and food waste (FW) were used as feedstock, and rice straw-derived biochar (RSB) was added as an additive to improve biogas production. The mesophilic anaerobic digestion resulted in a methane yield of 287.5 ml/g VS for FS + FW co-digestion and 396 ml/g VS for FS + FW + RSB co-digestion. At ambient temperature (20–26°C), the system produced a methane yield of 234 ml/g VS for FS + FW co-digestion and 275 ml/g VS for FS + FW + RSB co-digestion. Three different scenarios (digester with volumes of 4, 100, and 400 m3, respectively) and strategies for FS treatment by AD in Tanzania were proposed and analyzed. These treatments can produce methane volumes of 1.95, 49.5, and 199.5 m3 with pay-back periods of 3, 5, and 15 years and net present values of + 28, +1,337, and +52,351 USD, respectively. The calculations also showed that the heat value from the produced biogas and energy needed to heat the digester at 26–37°C resulted in energy balance values of + 0.012, + 0.53, and + 2.22 GJ/day for the 4, 100, and 400 m3 digester volumes, respectively.
Green synthesis of silver nanoparticles from vegetable waste of pea Pisum sativum and bottle gourd Lagenaria siceraria: Characterization and antibacterial properties
Authors: Deepa  , Ameen Fuad , Amirul Islam M. , Dhanker Raunak
Published: August 2022

A huge amount of food waste is being generated every day globally. Usually, India generates ∼350 million tons of food waste every year. Therefore, there is an urgent need to initiate research focusing on the management and hygienic methods of reuse of food waste together with advanced user-friendly methods of converting it into some useful products thereby generating wealth from food waste. A promising approach seems to biosynthesize silver nanoparticles (AgNPs) from such unutilized food. An alternative clean technology does not rely on the use of toxic chemicals and solvents. It is commonly allied with traditional nanoparticle synthesis processes. In the present work, the peels of two vegetables, pea (Pisum sativum) and bottle gourd (Lagenaria siceraria), were used to generate AgNPs. AgNPs were obtained by dissolving 1.5 ml of the peel extract of each vegetable in 50 ml of silver nitrate (AgNO3) and incubating for 24 h at room temperature. For the confirmation of AgNP production UV–visible spectroscopy was used. Field Emission Scanning Electron Microscopy (FESEM), X-ray diffraction (XRD), and attenuated total reflection-infrared spectroscopy (ATR-IR Analysis) were used to characterize them. Furthermore, AgNPs in different concentrations were used to test antibacterial activity against bacteria Escherichia coli through the disc diffusion method. Thus, our research indicates that AgNPs can be a safe and environmentally beneficial production technology from unutilized vegetable wastes that may play an important role in the management of food waste in the future and has antibacterial potential to preserve vegetables from bacterial contamination.
AEROsol generic classification using a novel Satellite remote sensing Approach (AEROSA)
Authors: Bilal Muhammad , Ali Md. Arfan , Nichol Janet E. , Bleiweiss Max P. , de Leeuw Gerrit , Mhawish Alaa , Shi Yuan , Mazhar Usman , Mehmood Tariq , Kim Jhoon , Qiu Zhongfeng , Qin Wenmin , Nazeer Majid
Published: August 2022

Numerous studies (hereafter GA: general approach studies) have been made to classify aerosols into desert dust (DD), biomass-burning (BB), clean continental (CC), and clean maritime (CM) types using only aerosol optical depth (AOD) and Ångström exponent (AE). However, AOD represents the amount of aerosol suspended in the atmospheric column while the AE is a qualitative indicator of the size distribution of the aerosol estimated using AOD measurements at different wavelengths. Therefore, these two parameters do not provide sufficient information to unambiguously classify aerosols into these four types. Evaluation of the performance of GA classification applied to AErosol Robotic NETwork (AERONET) data, at sites for situations with known aerosol types, provides many examples where the GA method does not provide correct results. For example, a thin layer of haze was classified as BB and DD outside the crop burning and dusty seasons respectively, a thick layer of haze was classified as BB, and aerosols from known crop residue burning events were classified as DD, CC, and CM by the GA method. The results also show that the classification varies with the season, for example, the same range of AOD and AE were observed during a dust event in the spring (20th March 2012) and a smog event in the autumn (2nd November 2017). The results suggest that only AOD and AE cannot precisely classify the exact nature (i.e., DD, BB, CC, and CM) of aerosol types without incorporating more optical and physical properties. An alternative approach, AEROsol generic classification using a novel Satellite remote sensing Approach (AEROSA), is proposed to provide aerosol amount and size information using AOD and AE, respectively, from the Terra-MODIS (MODerate resolution Imaging Spectroradiometer) Collection 6.1 Level 2 combined Dark Target and Deep Blue (DTB) product and AERONET Version 3 Level 2.0 data. Although AEROSA is also based on AOD and AE, it does not claim the nature of aerosol types, instead providing information on aerosol amount and size. The purpose is to introduce AEROSA for those researchers who are interested in the generic classification of aerosols based on AOD and AE, without claiming the exact aerosol types such as DD, BB, CC, and CM. AEROSA not only provides 9 generic aerosol classes for all observations but can also accommodate variations in location and season, which GA aerosol types do not.
Mechanisms governing the accessibility of DNA damage proteins to constitutive heterochromatin
Authors: Roemer Anastasia , Mohammed Lanah , Strickfaden Hilmar , Underhill D. Alan , Hendzel Michael J.
Published: August 2022

Chromatin is thought to regulate the accessibility of the underlying DNA sequence to machinery that transcribes and repairs the DNA. Heterochromatin is chromatin that maintains a sufficiently high density of DNA packing to be visible by light microscopy throughout the cell cycle and is thought to be most restrictive to transcription. Several studies have suggested that larger proteins and protein complexes are attenuated in their access to heterochromatin. In addition, heterochromatin domains may be associated with phase separated liquid condensates adding further complexity to the regulation of protein concentration within chromocenters. This provides a solvent environment distinct from the nucleoplasm, and proteins that are not size restricted in accessing this liquid environment may partition between the nucleoplasm and heterochromatin based on relative solubility. In this study, we assessed the accessibility of constitutive heterochromatin in mouse cells, which is organized into large and easily identifiable chromocenters, to fluorescently tagged DNA damage response proteins. We find that proteins larger than the expected 10 nm size limit can access the interior of heterochromatin. We find that the sensor proteins Ku70 and PARP1 enrich in mouse chromocenters. At the same time, MRE11 shows variability within an asynchronous population that ranges from depleted to enriched but is primarily homogeneously distribution between chromocenters and the nucleoplasm. While larger downstream proteins such as ATM, BRCA1, and 53BP1 are commonly depleted in chromocenters, they show a wide range of concentrations, with none being depleted beyond approximately 75%. Contradicting exclusively size-dependent accessibility, many smaller proteins, including EGFP, are also depleted in chromocenters. Our results are consistent with minimal size-dependent selectivity but a distinct solvent environment explaining reduced concentrations of diffusing nucleoplasmic proteins within the volume of the chromocenter.
The worldwide spread of Aedes albopictus: New insights from mitogenomes
Authors: Battaglia Vincenza , Agostini Vincenzo , Moroni Elisabetta , Colombo Giulia , Lombardo Gianluca , Rambaldi Migliore Nicola , Gabrieli Paolo , Garofalo Maria , Gagliardi Stella , Gomulski Ludvik M. , Ferretti Luca , Semino Ornella , Malacrida Anna R. , Gasperi Giuliano , Achilli Alessandro , Torroni Antonio , Olivieri Anna
Published: August 2022

The tiger mosquito (Aedes albopictus)is one of the most invasive species in the world and a competent vector for numerous arboviruses, thus the study and monitoring of its fast worldwide spread is crucial for global public health. The small extra-nuclear and maternally-inherited mitochondrial DNA represents a key tool for reconstructing phylogenetic and phylogeographic relationships within a species, especially when analyzed at the mitogenome level. Here the mitogenome variation of 76 tiger mosquitoes, 37 of which new and collected from both wild adventive populations and laboratory strains, was investigated. This analysis significantly improved the global mtDNA phylogeny ofAe. albopictus, uncovering new branches and sub-branches within haplogroup A1, the one involved in its recent worldwide spread. Our phylogeographic approach shows that the current distribution of tiger mosquito mitogenome variation has been strongly affected by clonal and sub-clonal founder events, sometimes involving wide geographic areas, even across continents, thus shedding light on the Asian sources of worldwide adventive populations. In particular, different starting points for the two major clades within A1 are suggested, with A1a spreading mainly along temperate areas from Japanese and Chinese sources, and A1b arising and mainly diffusing in tropical areas from a South Asian source.
The ECRG4 cleavage product augurin binds the endotoxin receptor and influences the innate immune response during otitis media
Authors: Kurabi Arwa , Hur Dong Gu , Pak Kwang , Gibson Madeline , Webster Nicholas J. G. , Baird Andrew , Eliceiri Brian P. , Ryan Allen F.
Published: August 2022

Otitis media (OM), the most common disease of childhood, is typically characterized by bacterial infection of the middle ear (ME). Prominent features of OM include hyperplasia of the ME mucosa, which transforms from a monolayer of simple squamous epithelium with minimal stroma into a full-thickness respiratory epithelium in 2–3 days after infection. Analysis of the murine ME transcriptome during OM showed down-regulation of the tumor suppressor gene Ecrg4 that was temporally related to mucosal hyperplasia and identified stromal cells as the primary ECRG4 source. The reduction in Ecrg4 gene expression coincided with the cleavage of ECRG4 protein to release an extracellular fragment, augurin. The duration of mucosal hyperplasia during OM was greater in Ecrg4−/− mice, the number of infiltrating macrophages was enhanced, and ME infection cleared more rapidly. ECRG4-null macrophages showed increased bacterial phagocytosis. Co-immunoprecipitation identified an association of augurin with TLR4, CD14 and MD2, the components of the lipopolysaccharide (LPS) receptor. The results suggest that full-length ECRG4 is a sentinel molecule that potentially inhibits growth of the ME stroma. Processing of ECRG4 protein during inflammation, coupled with a decline in Ecrg4 gene expression, also influences the behavior of cells that do not express the gene, limiting the production of growth factors by epithelial and endothelial cells, as well as the activity of macrophages.
CRISPR-Based Genome Editing for Nutrient Enrichment in Crops: A Promising Approach Toward Global Food Security
Authors: Kumar Dileep , Yadav Anurag , Ahmad Rumana , Dwivedi Upendra Nath , Yadav Kusum
Published: August 2022

The global malnutrition burden imparts long-term developmental, economic, social, and medical consequences to individuals, communities, and countries. The current developments in biotechnology have infused biofortification in several food crops to fight malnutrition. However, these methods are not sustainable and suffer from several limitations, which are being solved by the CRISPR-Cas-based system of genome editing. The pin-pointed approach of CRISPR-based genome editing has made it a top-notch method due to targeted gene editing, thus making it free from ethical issues faced by transgenic crops. The CRISPR-Cas genome-editing tool has been extensively used in crop improvement programs due to its more straightforward design, low methodology cost, high efficiency, good reproducibility, and quick cycle. The system is now being utilized in the biofortification of cereal crops such as rice, wheat, barley, and maize, including vegetable crops such as potato and tomato. The CRISPR-Cas-based crop genome editing has been utilized in imparting/producing qualitative enhancement in aroma, shelf life, sweetness, and quantitative improvement in starch, protein, gamma-aminobutyric acid (GABA), oleic acid, anthocyanin, phytic acid, gluten, and steroidal glycoalkaloid contents. Some varieties have even been modified to become disease and stress-resistant. Thus, the present review critically discusses CRISPR-Cas genome editing-based biofortification of crops for imparting nutraceutical properties.