bluuebunny/crossref_metadata_2025 huggingface dataset:
Neurobiology and medico-legal aspects of suicides among older adults: a narrative review
Authors: Sessa Francesco , Polito Rita , Li Rosi Giuseppe , Salerno Monica , Esposito Massimiliano , Pisanelli Daniela , Ministeri Federica , Messina Antonietta , Carotenuto Marco , Chieffi Sergio , Messina Giovanni , Monda Marcellino
Published: September 2024

The task of preventing suicide in older adults is an important social burden as older adults aged above 65 are exposed to singular psychological aspects that increase suicide risks. Moreover, when an older adult corpse is found, the medico-legal inspection represents a fundamental tool to identify the exact cause of death, classifying or excluding it as suicide. In this scenario, this review aims to explore the neurobiological factors that could be related to suicidal behavior in older adults. A further goal of this review is the exploration of the medico-legal aspects surrounding older adult suicides, clarifying the importance of forensic investigation. Particularly, this review examines issues such as neurotransmitter imbalances, cognitive impairment, neuroinflammation, psychosocial factors related to geriatric suicide, and neurodegenerative diseases. Additionally, medico-legal aspects such as policy considerations, legal frameworks, mental health assessments, ethical implications and forensic investigation were explored. Considering the importance of this phenomenon, especially in western countries, a need has emerged for focused screening tools on suicidal behavior among older adults, in order to contain it. Therefore, this review makes an exhaustive appraisal of the literature giving insights into the delicate interplay between neurobiology as well as mental health in relation to older adult suicide within a medico-legal context. The comprehension of different aspects about this complex phenomenon is fundamental to propose new and more effective interventions, supporting tailored initiatives such as family support and improving healthcare, specifically towards vulnerable ageing societies to reduce older adult suicide risks.
Intersectional configuration of infant mortality due to malnutrition in Colombia: a mini-review
Authors: Taborda Restrepo Paula A.
Published: September 2024

This mini review delves into the complex issue of mortality linked to malnutrition, highlighting its multifaceted nature beyond just biomedical factors, presenting it as an intricate intersectional phenomenon.

Method

The mini-review methodology involved a systematic literature search across databases like PubMed and Scielo, focusing on malnutrition and infant mortality in Colombia. We used specific keywords and Boolean operators to identify relevant studies, emphasizing socio-economic, gender, and ethnic factors, while excluding non-peer-reviewed and outdated publications.

Results

The relationship between gender and food/nutrition has deep historical and cultural roots. Patriarchal norms influence dietary habits based on gender roles, often placing undue responsibility on mothers for children’s nutritional health, reflecting profound social intersections. Mortality due to malnutrition is most prevalent among indigenous and Afro-descendant children in rural, conflict-affected areas with limited access to healthcare. Unpaid domestic work restricts women’s economic independence, intensifying challenges for single-parent households.

Conclusion

A comprehensive understanding can shift institutional attitudes toward mothers, leading to more coherent policy strategies and effective interventions.
Human animal relationships in Bos indicus cattle breeds addressed from a Five Domains welfare framework
Authors: Mota-Rojas Daniel , Whittaker Alexandra L. , Strappini Ana C. , Orihuela Agustín , Domínguez-Oliva Adriana , Mora-Medina Patricia , Álvarez-Macías Adolfo , Hernández-Avalos Ismael , Olmos-Hernández Adriana , Reyes-Sotelo Brenda , Grandin Temple
Published: September 2024

The present review has two objectives, the first is to investigate the differences in temperament between Bos indicus and Bos taurus breeds and determining the effects on production due to positive treatment and to compare this with negative HAR, by using the Five Domain Model as framework. The second objective is to discuss potential strategies to achieve better HAR when working with Bos indicus cattle. Bos indicus are more reactive and temperamental than Bos taurus cattle. When human animal relationships (HAR) are evaluated, Bos indicus cattle may react with greater intensity. They may be more likely to develop a negative emotional state, especially in extensively raised Bos indicus cattle that are handled only a few times each year. Bos indicus cattle can have positive emotional states when they have frequent positive interactions with people. Interactions with people, both positive and negative, would be in the fourth Domain of the Five Domains model. Cattle that are more reactive during handling may also have lower weight gain, even when they have abundant feed. This would be in the first Domain of Nutrition. When cattle are handled in races and corrals, injuries may be more likely to occur. Injuries and bruises would be in the third Domain of Health. Injuries could be caused by either poor handling practices by people or poor handling facilities. Yelling or electric prod use would be examples of poor HAR. Second Environmental Domain issues may be broken facilities or slick, slippery floors that are associated with falls.
Exploring swine oviduct anatomy through micro-computed tomography: a 3D modeling perspective
Authors: Belda-Perez Ramses , Cimini Costanza , Valbonetti Luca , Orsini Tiziana , D’Elia Annunziata , Massari Roberto , Di Carlo Carlo , Paradiso Alessia , Maqsood Seerat , Scavizzi Ferdinando , Raspa Marcello , Bernabò Nicola , Barboni Barbara
Published: September 2024

The oviduct plays a crucial role in the reproductive process, serving as the stage for fertilization and the early stages of embryonic development. When the environment of this organ has been mimicked, it has been shown to enhance in vitro embryo epigenetic reprogramming and to improve the yield of the system. This study explores the anatomical intricacies of two oviduct regions, the uterotubal junction (UTJ) and the ampullary-isthmic junction (AIJ) by using micro-computed tomography (MicroCT). In this study, we have characterized and 3D-reconstructed the oviduct structure, by measuring height and width of the oviduct’s folds, along with the assessments of fractal dimension, lacunarity and shape factor. Results indicate distinct structural features in UTJ and AIJ, with UTJ displaying small, uniformly distributed folds and high lacunarity, while AIJ shows larger folds with lower lacunarity. Fractal dimension analysis reveals values for UTJ within 1.189–1.1779, while AIJ values range from 1.559–1.770, indicating differences in structural complexity between these regions. Additionally, blind sacs or crypts are observed, akin to those found in various species, suggesting potential roles in sperm sequestration or reservoir formation. These morphological differences align with functional variations and are essential for developing an accurate 3D model. In conclusion, this research provides information about the oviduct anatomy, leveraging MicroCT technology for detailed 3D reconstructions, which can significantly contribute to the understanding of geometric-morphological characteristics influencing functional traits, providing a foundation for a biomimetic oviduct-on-a-chip.
Growth Performance, Meat Quality, and Lipid Oxidation in Pigs’ Fed Diets Containing Grape Pomace
Authors: da Silveira Almeida Barbara Cristina , Ludke Maria do Carmo Mohaupt Marques , Bertol Teresinha Marisa , Ludke Jorge Vitor , Bernardi Daniela Miotto , Cunha Jr. Anildo , Coldebella Arlei
Published: September 2024

Grape pomace is a winery byproduct that is rich in polyphenols with antioxidant capacity. This study investigated the effect of 0, 5, and 10% inclusion of dehydrated grape pomace (DGP) in finishing pig diets on the growth performance, carcass traits, fatty acid profile, fresh meat quality, and fat stability of a local pig genotype. A total of 36 pigs, 18 barrows, and 18 gilts (83.23 ± 6.03 kg and 132.1 ± 5.6 days old) were allotted in a randomized block design considering the initial weight (block) within sex, with six replicates of each sex per treatment. Including DGP in the diets did not affect daily weight gain or the feed-to-gain ratio; however, daily feed intake increased linearly (p < 0.05) and backfat thickness at the last rib, backfat thickness at the first sacral vertebrae, P2 backfat thickness, fat area, and the percentage of lean meat decreased linearly (p < 0.05) in pigs. The inclusion of DGP in pig diets did not affect the antioxidant potential evaluated by thiobarbituric acid-reactive substances in mini hamburgers or the quality characteristics of fresh meat, except for intramuscular fat (EE). The dietary inclusion of DGP linearly increased (p < 0.05) EE, saturated fatty acids, monounsaturated fatty acids, and Σω-3 and reduced the ω-6:ω-3 ratio in a linear way (p < 0.05) in the loin of pigs. We concluded that it is feasible to include up to 10% of DGP in pig diets without affecting growth performance, but carcass quality may be impaired due to increased adiposity. Furthermore, meat quality can be improved by increasing intramuscular fat and ω-3 fatty acid content, but fat stability is not affected when DGP is included at up to 10% of the diet for 49 days prior to slaughter.
Implications of Cash Transfer Programs for Mental Health Promotion among Families Facing Significant Stressors: Using Ecological Systems Theory to Explain Successes of Conditional and Unconditional Programs
Authors: Lesser Tali L. , Matalon Maya , Clauss-Ehlers Caroline S.
Published: September 2024

The purpose of this paper is to apply Bronfenbrenner’s ecological systems theory to explore the literature on how Conditional Cash Transfer (CCT) and Unconditional Cash Transfer (UCT) programs might support positive mental health outcomes. The paper begins with transnational considerations of stress, such as poverty and COVID-19, and their impact on mental health. Bronfenbrenner’s theory is applied to better understand the mechanisms by which CCT and UCT programs can potentially lead to positive outcomes for children and families who face such stressors. The implications of cash transfer programs are subsequently discussed in terms of how they might promote positive mental health outcomes among families globally. This theoretical application paper concludes with a call for transnational research to explore connections between cash transfer programs and mental health outcomes for children/adolescents and their parents/caregivers.
Dielectric Response of Asphalt Mixtures and Relationship to Air Voids and Stiffness
Authors: Georgouli Konstantina , Plati Christina
Published: September 2024

Asphalt mix air void content is a dominant parameter for asphalt mix design. The air void content of the mix affects the mechanical property of stiffness, while both characterize compacted asphalt mix materials. On the other hand, asphalt mix as a composite material can be characterized by its dielectric value. Considering the above, the aim of the present paper is to develop a simple methodology for the characterization of asphalt mix materials using their dielectric properties through an investigation of the interaction of dielectrics and air voids, as well as air voids and stiffness. For this purpose, an experimental laboratory study was conducted, which involved the compaction of asphalt mixes with different aggregate types and air void content. Upon this, the specimens were tested for their air void content, the dielectric constant, and the stiffness modulus. The analysis of the results showed strong correlations between the three characteristics. These findings were further verified with a new set of specimens and laboratory measurements. The final goal is to use the developed methodology for the estimation of asphalt mix stiffness considering that the effect of air content on the resulting stiffness cause indirect relationships between stiffness and dielectrics.
A New Approach to Examine the Dynamics of Switched-Mode Step-Up DC–DC Converters—A Switched State-Space Model
Authors: Tomaszuk Adam , Borawski Kamil
Published: September 2024

Power electronic converters are important elements of many modern devices. Therefore, there is a need for a thorough analysis of their behavior and the ability to properly control them. Typically, the converter’s dynamics are investigated using the small-signal averaging method, which does not provide detailed information about the converter. In particular, it does not account for the switching ripple effect. In this paper, a novel switched state–space model of the interleaved step-up DC–DC converter is introduced. That model incorporates high-frequency information, which allows for a more in-depth dynamics analysis. The results, i.e., step and frequency responses, obtained from both theoretical models are compared to the interleaved step-up DC–DC converter model implemented in PSpice ver. 16.6 from Cadence Design Systems.
Effects of the Disbursement of EU Cohesion Policy 2014–2020 Funds on Improving the Energy Efficiency of Buildings in Poland and Germany
Authors: Kociuba Dagmara , Janczak Maciej
Published: September 2024

Improving the energy efficiency of buildings (IEEBs) is crucial to achieving the European Union’s ambitious climate and energy goals. To channel these efforts, new policies, legislation, sources of funding, mechanisms for distributing funds, programs, and support tools are being introduced. The purpose of this study is to demonstrate the effects of the disbursement of EU Cohesion Policy 2014–2020 funds on IEEBs in Poland and Germany, filling the research gap regarding the regional differentiation of investments, beneficiaries, and the spatial distribution of subsidies and projects. An analysis was made of 7818 projects on IEEBs implemented under regional operational programs from European Regional Development Funds under Thematic Objective 4. This research was conducted for two main variables: (1) beneficiaries (8 categories) and (2) investments (8 categories). Based on this division, using a cluster analysis through the use of Ward’s agglomerative hierarchical clustering method, groupings were made in relation to beneficiaries (6 groups) and investments (6 group). The results allowed us to identify regional differences and similarities in the approach to the disbursement of funds on IEEBs, to indicate the main beneficiaries, to highlight trends in this regard, to demonstrate the impact of changes in territorial affiliation on investment targeting, and, furthermore, to capture the variation in the sophistication of the implementation of EU IEEB-related policies and legislation. The results provide a well-documented voice in the discussion of the effects of distributing EU funds for IEEBs in the regions of Poland and Germany and provide a basis for further comparative research.
Sustainable Biodiesel Production via Biogenic Catalyzed Transesterification of Baobab Oil Methyl Ester and Optimization Process
Authors: Etim Anietie , Musonge Paul
Published: September 2024

Biomass diesel is one of the sustainable and renewable sources of energy envisaged to hold a prominent position in the world energy infrastructure. In this study, biodiesel was produced from baobab seed oil by transesterification using biogenic heterogeneous catalyst, derived from mixed wastes of white chicken eggshells and banana fruit peels. The production process was statistically analyzed using Box-Behnken Design-Response Surface Methodology (BBD-RSM). The influential transesterification reaction parameters investigated with their ranges include reaction time (40–80 min), molar ratio of oil to methanol (1:9–1:15) and catalyst weight (3–5 wt%). The nano-catalyst (CaO-BFP-850 NPs) was prepared by calcination at high temperature of 850 °C for 4 h, and its properties were found to contain majorly the basic elements of Ca and K when investigated with analytical instruments such as SEM, EDS, DSC-TGA, FT-IR, and XRD. The regeneration test of the CaO-BFP-850 NPs conducted showed it could be reused for more than four cycles with less catalytic efficiency reduction. The ideal conditions instituted by BBD-RSM was 75 min of reaction time, 12.8:1 molar ratio of oil to methanol, and 4.08 wt% CaO-BFP-850 at 65 °C and 650 rpm constant temperature and agitation speed respectively, with the validated biodiesel yield of 96.70 wt%. The assessment of the quality of the biodiesel produced showed compliance with the standard specifications of ASTM D6751, EN 14241, and SANS 833.
Machine Learning-Driven Quantification of CO2 Plume Dynamics at Illinois Basin Decatur Project Sites Using Microseismic Data
Authors: Iyegbekedo Ikponmwosa , Fathi Ebrahim , Carr Timothy R. , Belyadi Fatemeh
Published: September 2024

This study utilizes machine learning to quantify CO2 plume extents by analyzing microseismic data from the Illinois Basin Decatur Project (IBDP). Leveraging a unique dataset of well logs, microseismic records, and CO2 injection metrics, this work aims to predict the temporal evolution of subsurface CO2 saturation plumes. The findings illustrate that machine learning can predict plume dynamics, revealing vertical clustering of microseismic events over distinct time periods within certain proximities to the injection well, consistent with an invasion percolation model. The buoyant CO2 plume partially trapped within sandstone intervals periodically breaches localized barriers or baffles, which act as leaky seals and impede vertical migration until buoyancy overcomes gravity and capillary forces, leading to breakthroughs along vertical zones of weakness. Between different unsupervised clustering techniques, K-Means and DBSCAN were applied and analyzed in detail, where K-means outperformed DBSCAN in this specific study by indicating the combination of the highest Silhouette Score and the lowest Davies–Bouldin Index. The predictive capability of machine learning models in quantifying CO2 saturation plume extension is significant for real-time monitoring and management of CO2 sequestration sites. The models exhibit high accuracy, validated against physical models and injection data from the IBDP, reinforcing the viability of CO2 geological sequestration as a climate change mitigation strategy and enhancing advanced tools for safe management of these operations.
Comparison of Likelihood Ratios from Probabilistic Genotyping for Two-Person Mixtures across Different Assays and Instruments
Authors: McNevin Dennis , Barash Mark
Published: September 2024

Continuous probabilistic genotyping (PG) provides a means to estimate the probative value of DNA mixtures tendered as evidence in court and subject to alternative propositions about the contributors to the mixtures. The weight of that evidence, however, may be valued differently, depending on which forensic laboratory undertook the DNA analysis. There is a need, therefore, to have a means for the comparison of likelihood ratios (LRs) generated by continuous PG amongst different laboratories for the same initial DNA sample. Such a comparison would enable the courts and the public to make judgements about the reliability of this type of evidence. There are particular mixtures and methods for which such a comparison is meaningful, and this study explores them for the short tandem repeat (STR) electropherograms of two-person mixtures obtained from the PROVEDIt Database. We demonstrate a common maximum attainable LR for a given set of common STR loci and a given DNA mixture that is consistent across three different STR profiling assays and two different capillary electrophoresis instruments.
Cardiometabolic Risk in a University Community: An Observational Study
Authors: Pérez-Manchón David , Barrio-Cortes Jaime , Vicario-Merino Angel , Mayoral-Gonzalo Noemí , Ruiz-López Montserrat , Corral-Pugnaire Eduardo , Blanco-Hermo Patricia , Ruiz-Zaldibar Cayetana
Published: September 2024

The highest prevalence of cardiovascular risk factors has been associated with obesity, sedentary lifestyle, and elevated blood pressure due to high workload and work stress. This study aimed to analyze the cardiometabolic risk and lifestyles among the health sciences university academics and campus administrators at a private university in Spain. A cross-sectional study was conducted during the 2018–2019 academic year by the Nursing Department, using a self-administered questionnaire and face-to-face assessments of anthropometric variables related to cardiovascular risk in university personnel. The variables measured included sociodemographics, cardiovascular risk history, comorbidities, toxic habits, Mediterranean diet adherence, physical exercise, psychosocial stress, and physical, anthropometric, and analytical data. Cardiovascular risk was categorized into relative (<40 years), absolute, and vascular age (>40 years). Among the 101 participants, 61.4% were women, with a mean age of 41.3 years ± 9 years. The smoking prevalence was 21.8% (68.2% women), 27.7% were sedentary, and 51.0% adhered to the Mediterranean diet, with higher adherence among the academics. Emotional risk was present in 32.7% of the participants. A prior diagnosis of hypertension was significantly more frequent in the men (15.4%) compared to the women (3.2%). The blood pressure measurements were mostly optimal across both genders and professional groups, but the proportion of hypertension grade 1 was significantly higher among the academics (10%) compared to the administrators (4.5%) and among the men (11.1%) compared to the women (5.9%). The absolute cardiovascular risk among the university employees was generally low, but the men exhibited a more moderate risk compared to the women. It is necessary for the university to promote health within its community, with the Nursing Department playing a key role in health promotion and research.
Advancing Hydrogen Gas Utilization in Industrial Boilers: Impacts on Critical Boiler Components, Mitigation Measures, and Future Perspectives
Authors: Honu Edem , Guo Shengmin , Rahman Shafiqur , Zeng Congyuan , Mensah Patrick
Published: September 2024

This review sets out to investigate the detrimental impacts of hydrogen gas (H2) on critical boiler components and provide appropriate state-of-the-art mitigation measures and future research directions to advance its use in industrial boiler operations. Specifically, the study focused on hydrogen embrittlement (HE) and high-temperature hydrogen attack (HTHA) and their effects on boiler components. The study provided a fundamental understanding of the evolution of these damage mechanisms in materials and their potential impact on critical boiler components in different operational contexts. Subsequently, the review highlighted general and specific mitigation measures, hydrogen-compatible materials (such as single-crystal PWA 1480E, Inconel 625, and Hastelloy X), and hydrogen barrier coatings (such as TiAlN) for mitigating potential hydrogen-induced damages in critical boiler components. This study also identified strategic material selection approaches and advanced approaches based on computational modeling (such as phase-field modeling) and data-driven machine learning models that could be leveraged to mitigate potential equipment failures due to HE and HTHA under elevated H2 conditions. Finally, future research directions were outlined to facilitate future implementation of mitigation measures, material selection studies, and advanced approaches to promote the extensive and sustainable use of H2 in industrial boiler operations.
Exploring the Anticancer Potential of Phenolic nor-Triterpenes from Celastraceae Species
Authors: Reyes Carolina P. , Ardiles Alejandro , Anaissi-Afonso Laura , González-Bakker Aday , Padrón José M. , Jiménez Ignacio A. , Machín Félix , Bazzocchi Isabel L.
Published: September 2024

To explore new compounds with antitumour activity, fifteen phenolic nor-tripterpenes isolated from Celastraceae species, Maytenus jelskii, Maytenus cuzcoina, and Celastrus vulcanicola, have been studied. Their chemical structures were elucidated through spectroscopic and spectrometric techniques, resulting in the identification of three novel chemical compounds. Evaluation on human tumour cell lines (A549 and SW1573, non-small cell lung; HBL-100 and T-47D, breast; HeLa, cervix, and WiDr, colon) revealed that three compounds, named 6-oxo-pristimerol, demethyl-zeylasteral, and zeylasteral, exhibited significant activity (GI50 ranging from 0.45 to 8.6 µM) on at least five of the cell lines tested. Continuous live cell imaging identified apoptosis as the mode of action of selective cell killing in HeLa cells. Furthermore, their effect on a drug-sensitive Saccharomyces cerevisiae strain has been investigated to deepen on their mechanism of action. In dose-response growth curves, zeylasteral and 7α-hydroxy-blepharodol were markedly active. Additionally, halo assays were conducted to assess the involvement of oxidative stress and/or mitochondrial function in the anticancer profile, ruling out these modes of action for the active compounds. Finally, we also delve into the structure-activity relationship, providing insights into how the molecular structure of these compounds influences their biological activity. This comprehensive analysis enhances our understanding of the therapeutic potential of this triterpene type and underscores its relevance for further research in this field.
A New Strategy for Ultrasensitive Detection Based on Target microRNA-Triggered Rolling Circle Amplification in the Early Diagnosis of Alzheimer’s Disease
Authors: Zhao Fei , Zhang Na , Zhang Yi
Published: September 2024

There is an urgent need to accurately quantify microRNA (miRNA)-based Alzheimer’s disease (AD) biomarkers, which have emerged as promising diagnostic biomarkers. In this study, we present a rapid and universal approach to establishing a target miRNA-triggered rolling circle amplification (RCA) detection strategy, which achieves ultrasensitive detection of several targets, including miR-let7a-5p, miR-34a-5p, miR-206-3p, miR-9-5p, miR-132-3p, miR-146a-5p, and miR-21-5p. Herein, the padlock probe contains three repeated signal strand binding regions and a target miRNA-specific region. The target miRNA-specific region captures miRNA, and then the padlock probe is circularized with the addition of T4 DNA ligase. Subsequently, an RCA reaction is triggered, and RCA products containing multiple signal strand binding regions are generated to trap abundant fluorescein-labeled signal strands. The addition of exonuclease III (Exo III) causes signal strand digestion and leads to RCA product recycling and liberation of fluorescein. Ultimately, graphene oxide (GO) does not absorb the liberated fluorescein because of poor mutual interaction. This method exhibited high specificity, sensitivity, repeatability, and stability toward let-7a, with a detection limit of 19.35 fM and a linear range of 50 fM to 5 nM. Moreover, it showed excellent applicability for recovering miRNAs in normal human serum. Our strategy was applied to detect miRNAs in the plasma of APP/PS1 mice, demonstrating its potential in the diagnosis of miRNA-associated disease and biochemical research.
The Inhibiting Effect of GB-2, (+)-Catechin, Theaflavin, and Theaflavin 3-Gallate on Interaction between ACE2 and SARS-CoV-2 EG.5.1 and HV.1 Variants
Authors: Lu Chung-Kuang , Lung Jrhau , Shu Li-Hsin , Liu Hung-Te , Wu Yu-Huei , Lin Yu-Shih , Yang Yao-Hsu , Wu Yu-Heng , Wu Ching-Yuan
Published: September 2024

The ongoing COVID-19 pandemic, caused by SARS-CoV-2, continues to pose significant global health challenges. The results demonstrated that GB-2 at 200 μg/mL effectively increased the population of 293T-ACE2 cells with low RBD binding for both SARS-CoV-2 Omicron EG.5.1 and HV.1 variants by dual-color flow cytometry, indicating its ability to inhibit virus attachment. Further investigation revealed that (+)-catechin at 25 and 50 μg/mL did not significantly alter the ACE2–RBD interaction for the EG.5.1 variant. In contrast, theaflavin showed inhibitory effects at both 25 and 50 μg/mL for EG.5.1, while only the higher concentration was effective for HV.1. Notably, theaflavin 3-gallate exhibited a potent inhibition of ACE2–RBD binding for both variants at both concentrations tested. Molecular docking studies provided insight into the binding mechanisms of theaflavin and theaflavin 3-gallate with the RBD of EG.5.1 and HV.1 variants. Both compounds showed favorable docking scores, with theaflavin 3-gallate demonstrating slightly lower scores (−8 kcal/mol) compared to theaflavin (−7 kcal/mol) for both variants. These results suggest stable interactions between the compounds and key residues in the RBD, potentially explaining their inhibitory effects on virus attachment. In conclusion, GB-2, theaflavin, and theaflavin 3-gallate demonstrate significant potential as inhibitors of the ACE2–RBD interaction in Omicron variants, highlighting their therapeutic promise against COVID-19. However, these findings are primarily based on computational and in vitro studies, necessitating further in vivo research and clinical trials to confirm their efficacy and safety in humans.
ToxDAR: A Workflow Software for Analyzing Toxicologically Relevant Proteomic and Transcriptomic Data, from Data Preparation to Toxicological Mechanism Elucidation
Authors: Jiang Peng , Zhang Zuzhen , Yu Qing , Wang Ze , Diao Lihong , Li Dong
Published: September 2024

Exploration of toxicological mechanisms is imperative for the assessment of potential adverse reactions to chemicals and pharmaceutical agents, the engineering of safer compounds, and the preservation of public health. It forms the foundation of drug development and disease treatment. High-throughput proteomics and transcriptomics can accurately capture the body’s response to toxins and have become key tools for revealing complex toxicological mechanisms. Recently, a vast amount of omics data related to toxicological mechanisms have been accumulated. However, analyzing and utilizing these data remains a major challenge for researchers, especially as there is a lack of a knowledge-based analysis system to identify relevant biological pathways associated with toxicity from the data and to establish connections between omics data and existing toxicological knowledge. To address this, we have developed ToxDAR, a workflow-oriented R package for preprocessing and analyzing toxicological multi-omics data. ToxDAR integrates packages like NormExpression, DESeq2, and igraph, and utilizes R functions such as prcomp and phyper. It supports data preparation, quality control, differential expression analysis, functional analysis, and network analysis. ToxDAR’s architecture also includes a knowledge graph with five major categories of mechanism-related biological entities and details fifteen types of interactions among them, providing comprehensive knowledge annotation for omics data analysis results. As a case study, we used ToxDAR to analyze a transcriptomic dataset on the toxicology of triphenyl phosphate (TPP). The results indicate that TPP may impair thyroid function by activating thyroid hormone receptor β (THRB), impacting pathways related to programmed cell death and inflammation. As a workflow-oriented data analysis tool, ToxDAR is expected to be crucial for understanding toxic mechanisms from omics data, discovering new therapeutic targets, and evaluating chemical safety.
Characterization of TBP and TAFs in Mungbean (Vigna radiata L.) and Their Potential Involvement in Abiotic Stress Response
Authors: Wu Ranran , Jia Qiyuan , Guo Yingjian , Lin Yun , Liu Jinyang , Chen Jingbin , Yan Qiang , Yuan Na , Xue Chenchen , Chen Xin , Yuan Xingxing
Published: September 2024

The TATA-box binding protein (TBP) and TBP-associated factors (TAFs) constitute the transcription factor IID (TFIID), a crucial component of RNA polymerase II, essential for transcription initiation and regulation. Several TFIID subunits are shared with the Spt–Ada–Gcn5–acetyltransferase (SAGA) coactivator complex. Recent research has revealed the roles of TBP and TAFs in organogenesis and stress adaptation. In this study, we identified 1 TBP and 21 putative TAFs in the mungbean genome, among which VrTAF5, VrTAF6, VrTAF8, VrTAF9, VrTAF14, and VrTAF15 have paralogous genes. Their potential involvement in abiotic stress responses was also investigated here, including high salinity, water deficit, heat, and cold. The findings indicated that distinct genes exerted predominant influences in the response to different abiotic stresses through potentially unique mechanisms. Specifically, under salt stress, VrTBP, VrTAF2, and VrTAF15–1 were strongly induced, while VrTAF10, VrTAF11, and VrTAF13 acted as negative regulators. In the case of water-deficit stress, it was likely that VrTAF1, VrTAF2, VrTAF5–2, VrTAF9, and VrTAF15–1 were primarily involved. Additionally, in response to changes in ambient temperature, it was possible that genes such as VrTAF5–1, VrTAF6–1, VrTAF9–2, VrTAF10, VrTAF13, VrTAF14b–2, and VrTAF15–1 might play a dominant role. This comprehensive exploration of VrTBP and VrTAFs can offer a new perspective on understanding plant stress responses and provide valuable insights into breeding improvement.
R2R3 MYB Transcription Factor GhMYB201 Promotes Cotton Fiber Elongation via Cell Wall Loosening and Very-Long-Chain Fatty Acid Synthesis
Authors: Suo Qingwei , Fang Nianjuan , Zeng Jianyan , Yan Fulin , Zhu Xi , Wang Yi , Yu Wanting , Chen Junmin , Liang Aimin , Li Yaohua , Kong Jie , Xiao Yuehua
Published: September 2024

Cotton fiber is the leading natural textile material, and fiber elongation plays an essential role in the formation of cotton yield and quality. Although a number of components in the molecular network controlling cotton fiber elongation have been reported, a lot of players still need to be functionally dissected to understand the regulatory mechanism of fiber elongation comprehensively. In the present study, an R2R3-MYB transcription factor gene, GhMYB201, was characterized and functionally verified via CRISPR/Cas9-mediated gene editing. GhMYB201 was homologous to Arabidopsis AtMYB60, and both coding genes (GhMYB201At and GhMYB201Dt) were preferentially expressed in elongating cotton fibers. Knocking-out of GhMYB201 significantly reduced the rate and duration of fiber elongation, resulting in shorter and coarser mature fibers. It was found that GhMYB201 could bind and activate the transcription of cell wall loosening genes (GhRDLs) and also β-ketoacyl-CoA synthase genes (GhKCSs) to enhance very-long-chain fatty acid (VLCFA) levels in elongating fibers. Taken together, our data demonstrated that the transcription factor GhMYB201s plays an essential role in promoting fiber elongation via activating genes related to cell wall loosening and VLCFA biosynthesis.
Analytical and Numerical Investigation of Star Polymers in Confined Geometries
Authors: Danel Zoriana , Halun Joanna , Karbowniczek Pawel
Published: September 2024

The analysis of the impact of the star polymer topology on depletion interaction potentials, depletion forces, and monomer density profiles is carried out analytically using field theory methods and techniques as well as molecular dynamic simulations. The dimensionless depletion interaction potentials and the dimensionless depletion forces for a dilute solution of ideal star polymers with three and five legs (arms) in a Θ-solvent confined in a slit between two parallel walls with repulsive surfaces and for the case where one of the surfaces is repulsive and the other inert are obtained. Furthermore, the dimensionless layer monomer density profiles for ideal star polymers with an odd number (f˜ = 3, 5) of arms immersed in a dilute solution of big colloidal particles with different adsorbing or repelling properties in respect of polymers are calculated, bearing in mind the Derjaguin approximation. Molecular dynamic simulations of a dilute solution of star-shaped polymers in a good solvent with N = 901 (3 × 300 + 1 -star polymer with three arms) and 1501 (5 × 300 + 1 -star polymer with five arms) beads accordingly confined in a slit with different boundary conditions are performed, and the results of the monomer density profiles for the above-mentioned cases are obtained. The numerical calculation of the radius of gyration for star polymers with f˜ = 3, 5 arms and the ratio of the perpendicular to parallel components of the radius of gyration with respect to the wall orientation for the above-mentioned cases is performed. The obtained analytical and numerical results for star polymers with an odd number (f˜ = 3, 5) of arms are compared with our previous results for linear polymers in confined geometries. The acquired results show that a dilute solution of star polymer chains can be applied in the production of new functional materials, because the behavior of these solutions is strictly correlated with the topology of polymers and also with the nature and geometry of confined surfaces. The above-mentioned properties can find extensive practical application in materials engineering, as well as in biotechnology and medicine for drug and gene transmission.
Characterization of the Bax Inhibitor-1 Family in Cauliflower and Functional Analysis of BobBIL4
Authors: Liu Xin , Guo Ning , Li Shasha , Duan Mengmeng , Wang Guixiang , Zong Mei , Han Shuo , Wu Zihan , Liu Fan , Zhang Junjie
Published: September 2024

The Bax inhibitor-1 (BI-1) gene family, which is important for plant growth, development, and stress tolerance, remains largely unexplored in cauliflower. In this study, we identified and characterized cauliflower BI-1 family genes. Based on aligned homologous sequences and collinearity with Arabidopsis genes, we identified nine cauliflower BI-1 genes, which encode proteins that varied in length, molecular weight, isoelectric point, and predicted subcellular localization, including the Golgi apparatus, plasma membrane, and various compartments within the chloroplast. Phylogenetic analyses detected evolutionary conservation and divergence among these genes. Ten structural motifs were identified, with Motif 5 found to be crucial for inhibiting apoptosis. According to the cis-regulatory elements in their promoters, these genes likely influence hormone signaling and stress responses. Expression profiles among tissues highlighted the functional diversity of these genes, with particularly high expression levels observed in the silique and root. Focusing on BobBIL4, we investigated its role in brassinosteroid (BR)-mediated root development and salt stress tolerance. BobBIL4 expression levels increased in response to BR and salt treatments. The functional characterization of this gene in Arabidopsis revealed that it enhances root growth and salinity tolerance. These findings provide insights into BI-1 gene functions in cauliflower while also highlighting the potential utility of BobBIL4 for improving crop stress resistance.
Fibrin Scaffolds Perfused with Transforming Growth Factor-β1 as an In Vitro Model to Study Healthy and Tendinopathic Human Tendon Stem/Progenitor Cells
Authors: Ciardulli Maria Camilla , Lovecchio Joseph , Parolini Ornella , Giordano Emanuele , Maffulli Nicola , Della Porta Giovanna
Published: September 2024

A limited understanding of tendon cell biology in healthy and pathological conditions has impeded the development of effective treatments, necessitating in vitro biomimetic models for studying tendon events. We established a dynamic culture using fibrin scaffolds, bioengineered with tendon stem/progenitor cells (hTSPCs) from healthy or diseased human biopsies and perfused with 20 ng/mL of human transforming growth factor-β1 for 21 days. Both cell types showed long-term viability and upregulated Scleraxis (SCX-A) and Tenomodulin (TNMD) gene expressions, indicating tenogenic activity. However, diseased hTSPCs underexpressed collagen type I and III (COL1A1 and COL3A1) genes and exhibited lower SCX-A and TNMD protein levels, but increased type I collagen production, with a type I/type III collagen ratio > 1.5 by day 14, matching healthy cells. Diseased hTSPCs also showed constant high levels of pro-inflammatory cytokines, such as IL-8 and IL-6. This biomimetic environment is a valuable tool for studying tenogenic and inflammatory events in healthy and diseased tendon cells and identifying new therapeutic targets.
A Systematic Review of Diagnostic Performance of Circulating MicroRNAs in Colorectal Cancer Detection with a Focus on Early-Onset Colorectal Cancer
Authors: AlZaabi Adhari , Shalaby Asem
Published: September 2024

The rising incidence and mortality of early-onset colorectal cancer (EOCRC) emphasize the urgent need for effective non-invasive screening. Circulating microRNAs (miRNAs) have emerged as promising biomarkers for cancer detection. This systematic review aims to evaluate the diagnostic performance of circulating miRNAs in detecting colorectal cancer (CRC). A literature search was conducted in PubMed and Scopus. Studies that report sensitivity, specificity, or area under the curve (AUC) for CRC detection by miRNA were included. The miRNA miR-21 was the most frequently studied biomarker, with a varying range of AUC from 0.55 to 0.973 attributed to differences in study populations and methodologies. The miRNAs miR-210 and miR-1246 showed potential diagnostic capacity with miR-1246 achieving an AUC of 0.924, 100% sensitivity, and 80% specificity. The miRNA panels offer improved diagnostic performance compared to individual miRNA. The best performing panel for CRC patients below 50 is miR-211 + miR-25 + TGF-β1 with AUC 0.99 and 100 specificity and 97 sensitivity. Circulating miRNAs hold significant promise as non-invasive biomarkers for CRC screening. However, the variability in diagnostic performance highlights the need for a standardized method and robust validation studies. Future research should focus on large-scale, ethnically diverse cohorts to establish clinically relevant miRNA biomarkers for CRC, particularly in younger populations.
The Influence of Shot Peening Media on Surface Properties and Fatigue Behaviour of Aluminium Alloy 6082 T6
Authors: Calvo-García Erik , del Val Jesús , Riveiro Antonio , Valverde-Pérez Sara , Álvarez David , Román Manuel , Magdalena César , Badaoui Aida , Pou-Álvarez Pablo , Comesaña Rafael
Published: September 2024

Shot peening is generally used to improve the fatigue performance of mechanical components. However, identifying the geometrical and mechanical characteristics of the shots that improve fatigue strength is still a challenging task, as there are many variables involved in the shot peening process. The present work addresses the effect of different shot media on the fatigue behaviour of an aluminium alloy 6082 T6. Four different shot types were used: silica microspheres, alumina shots, aluminium cut wire and zinc cut wire. Axial fatigue tests were carried out to obtain the Wöhler curves corresponding to each shot peening treatment. The surface properties of the shot-peened specimens, such as grain size, hardness, residual stress and roughness were measured to determine their effect on the fatigue results. The fatigue results revealed that silica and zinc shots increased significantly the fatigue life of the alloy, whereas alumina and aluminium shots reduced its fatigue strength. Almen intensities have shown to correlate well with grain refinement and strain hardening. However, better fatigue results were obtained with the shots that generated higher surface compressive residual stresses. It is believed that small and smooth shots are preferable to sharp and irregular ones, regardless of the Almen intensity or surface hardness attained with the latter.
Research on Influencing Factors of Cable Clamp Bolt Elastic Interaction in Cross-Ocean Suspension Bridges
Authors: Mu Fengrui , Wang Bo , Zhou Yongjun , Jing Yuan , Zhao Yu , Luo Zhiran
Published: September 2024

Suspension bridges are the most common type of bridge used to cross the ocean. The cable clamps in suspension bridges clamp the main cables by bolt preload, but the elastic interaction of the bolts reduces the preload, which is detrimental to the force in suspension bridges. However, research on the factors influencing the elastic interaction of cable clamp bolts in suspension bridges is currently limited. This paper aims to explore the law of influence of external factors on the elastic interaction of bolts through a combined approach of theoretical analysis, full-scale experiment, and finite element simulation. The results indicate that the average preload loss was reduced by about 27% when the elastic modulus was increased by about 110%. The average preload loss was reduced by about 45% when the bolt center distance was increased by 75%. The number of bolts has a small effect on the elastic interaction, which can be ignored. When the preload of bolt installation was increased by 133%, the average preload loss was reduced by approximately 125%, which was almost a linear relationship. Tightening the bolt from the center bolt creates greater elastic interaction. The conclusions can provide suggestions for reducing the elastic interaction of bolts in the design and construction of suspension bridge cable clamps.
Short and Long-Term Outcomes of Transcatheter Aortic Valve Implantation in the Small Aortic Annulus: A Systematic Literature Review
Authors: Cabrucci Francesco , Baudo Massimo , Yamashita Yoshiyuki , Dokollari Aleksander , Sicouri Serge , Ramlawi Basel
Published: September 2024

Transcatheter aortic valve implantation has revolutionized the treatment of aortic stenosis. The small aortic annulus is one of the most challenging aspects of aortic stenosis treatment and since the beginning, TAVI has shown promising results in this subgroup of patients. This systematic literature review aims to investigate the short and long-term outcomes of TAVI in the small aortic annulus. The literature was meticulously screened for this topic until April 2024 using the PRISMA guidelines. Technical aspects, characteristics of this subgroup of patients, hemodynamic performances, and outcomes are discussed. The importance of device selection has shown up, with insight into the differences between self-expandable and the balloon-expandable valves. Two special populations were also taken into account: outcomes of TAVI in the small aortic annulus with bicuspid aortic valve and extra-small aortic annulus. The last 10 years have been paramount in technological advancements, bringing TAVI to broader use in this population. While several important trials underscored the usefulness of TAVI in the small aortic annulus population, the clinical practice still lacks consensus on the ideal device, and the outcomes are debated. The pivotal role of TAVI in this context needs to be addressed with a patient-tailored approach to optimize patient care.
On the Solute-Induced Structure-Making/Breaking Phenomena: Myths, Verities, and Misuses in Solvation Thermodynamics
Authors: Chialvo Ariel A.
Published: September 2024

We review the statistical mechanic foundations of the fundamental structure-making/breaking functions, leading to the rigorous description of the solute-induced perturbation of the solvent environment for the understanding of the solvation process of any species regardless of the type and nature of the solute–solvent interactions. Then, we highlight how these functions are linked to unambiguous thermodynamic responses resulting from changes in state conditions, composition, and solute–solvent intermolecular interaction asymmetries. Finally, we identify and illustrate the pitfalls behind the use of surrogate approaches to structure-making/breaking markers, including those based on Jones–Dole’s B-coefficient and Hepler’s isobaric-thermal expansivity, while highlighting their ambiguities and lack of consistency and the sources of misinterpretations.