bluuebunny/crossref_metadata_2025 huggingface dataset:
An antibiotic produced by Pseudomonas fluorescens CFBP2392 with antifungal activity against Rhizoctonia solani
Authors: Riera Nadia , Davyt Danilo , Durán Rosario , Iraola Gregorio , Lemanceau Philippe , Bajsa Natalia
Published: November 2023

Pseudomonas fluorescens CFBP2392 has been recognized as a potential biocontrol agent due to its ability to suppress damping-off and root rot disease. This isolate has antibacterial activity in vitro as many other strains from the Pseudomonas fluorescens complex. In this work, the antibacterial and antifungal activity of the strain were explored. Dual culture assays evidenced the antifungal activity of the strain against different phytopathogens: Alternaria sp., Pythium ultimun, Fusarium oxysporum, and Rhizoctonia solani. Purification of an antifungal fraction was performed by preparative HPLC from the chemical extraction of growth media. The fraction showed altered R. solani growth and ultrastructure. Transmission electron microscopy revealed the purified compound induced hypertrophied mitochondria, membranous vesicles, and a higher number of vacuoles in R. salani cytoplasm. In addition, co-cultivation of P. fluorescens CFBP2392 with R. solani resulted in an enlarged and deformed cell wall. To gain genomic insights on this inhibition, the complete genome of P. fluorescens CFBP2392 was obtained with Oxford Nanopore technology. Different biosynthetic gene clusters (BGCs) involved in specialized metabolites production including a lokisin-like and a koreenceine-like cluster were identified. In accordance with the putative BGCs identified, sequence phylogeny analysis of the MacB transporter in the lokisin-like cluster further supports the similarity with other transporters from the amphisin family. Our results give insights into the cellular effects of the purified microbial metabolite in R. solani ultrastructure and provide a genomic background to further explore the specialized metabolite potential.
Role of environmental enrichment on social interaction, anxiety, locomotion, and memory in Wistar rats under chronic methylphenidate intake
Authors: Herrera-Isaza Laura , Zárate-Guerrero Santiago , Corredor Karen , Gómez-Fonseca Ángela , Escobar-Cornejo Guillermo , Cardenas Fernando P.
Published: November 2023

Introduction

Chronic use of various compounds can have long-lasting effects on animal behavior, and some of these effects can be influenced by the environment. Many environmental enrichment protocols have the potential to induce behavioral changes.

Aim

The aim of the present study was to investigate how environmental enrichment can mitigate the effects of chronic methylphenidate consumption on the behavior of Wistar rats.

Methods

The animals were housed for 20 days under either an environmental enrichment protocol (which included tubes of different shapes) or standard housing conditions. After seven days, half of the rats received 13 days of oral administration of methylphenidate (2 mg/kg). After seven days, the rats underwent behavioral tests, including the elevated plus maze (anxiety), open field (locomotion), object-in-place recognition test (spatial memory), and a test for social interaction (social behavior).

Results

The results showed that the enriched environmental condition reversed the enhanced time in the open arms of the elevated plus maze induced by methylphenidate (F[1,43] = 4.275, p = 0.045). Methylphenidate also enhanced exploratory rearing in the open field (F[1,43] = 4.663, p = 0.036) and the time spent in the open area of the open field (H[3] = 8.786, p = 0.032). The enriched environment mitigated the inhibition of social interaction with peers induced by methylphenidate (H[3] = 16.755, p  < 0.001) as well as the preference for single exploratory behavior (H[3] = 9.041, p = 0.029).

Discussion

These findings suggest that environmental enrichment can counteract some of the effects of methylphenidate. These results are relevant for the clinical treatment of the long-lasting secondary effects associated with methylphenidate pharmacological treatment.
Neuronal models of TDP-43 proteinopathy display reduced axonal translation, increased oxidative stress, and defective exocytosis
Authors: Pisciottani Alessandra , Croci Laura , Lauria Fabio , Marullo Chiara , Savino Elisa , Ambrosi Alessandro , Podini Paola , Marchioretto Marta , Casoni Filippo , Cremona Ottavio , Taverna Stefano , Quattrini Angelo , Cioni Jean-Michel , Viero Gabriella , Codazzi Franca , Consalez G. Giacomo
Published: November 2023

Amyotrophic lateral sclerosis (ALS) is a progressive, lethal neurodegenerative disease mostly affecting people around 50–60 years of age. TDP-43, an RNA-binding protein involved in pre-mRNA splicing and controlling mRNA stability and translation, forms neuronal cytoplasmic inclusions in an overwhelming majority of ALS patients, a phenomenon referred to as TDP-43 proteinopathy. These cytoplasmic aggregates disrupt mRNA transport and localization. The axon, like dendrites, is a site of mRNA translation, permitting the local synthesis of selected proteins. This is especially relevant in upper and lower motor neurons, whose axon spans long distances, likely accentuating their susceptibility to ALS-related noxae. In this work we have generated and characterized two cellular models, consisting of virtually pure populations of primary mouse cortical neurons expressing a human TDP-43 fusion protein, wt or carrying an ALS mutation. Both forms facilitate cytoplasmic aggregate formation, unlike the corresponding native proteins, giving rise to bona fide primary culture models of TDP-43 proteinopathy. Neurons expressing TDP-43 fusion proteins exhibit a global impairment in axonal protein synthesis, an increase in oxidative stress, and defects in presynaptic function and electrical activity. These changes correlate with deregulation of axonal levels of polysome-engaged mRNAs playing relevant roles in the same processes. Our data support the emerging notion that deregulation of mRNA metabolism and of axonal mRNA transport may trigger the dying-back neuropathy that initiates motor neuron degeneration in ALS.
Neuropathological features of levodopa-responsive parkinsonism in multiple system atrophy: an autopsy case report and comparative neuropathological study
Authors: Tamura Mitsuyoshi , Takeda Takahiro , Kitayama Yoshihisa , Suichi Tomoki , Shibuya Kazumoto , Harada-Kagitani Sakurako , Kishimoto Takashi , Kuwabara Satoshi , Hirano Shigeki
Published: November 2023

Background

In typical patients with multiple system atrophy with predominant parkinsonism (MSA-P) levodopa is ineffective. However, there are some of these patients who respond well to levodopa treatment. Levodopa efficacy in MSA-P patients is thought to be related to the degree of putaminal damage, but the pathological causation between the putaminal involvement and levodopa efficacy has not been established in detail.

Objective

This study aimed to evaluate the neuropathological features of the nigrostriatal dopaminergic system in a “levodopa-responsive” MSA-P patient in comparison with “levodopa-unresponsive” conventional MSA-P patients.

Materials and methods

Clinicopathological findings were assessed in a 53-year-old Japanese man with MSA who presented with asymmetric parkinsonism, levodopa response, and later wearing-off phenomenon. During autopsy, the nigrostriatal pathology of presynaptic and postsynaptic dopaminergic receptor density and α-synuclein status were investigated. The other two patients with MSA-P were examined using the same pathological protocol.

Results

Four years after the onset, the patient died of sudden cardiopulmonary arrest. On autopsy, numerous α-synuclein-positive glial cytoplasmic inclusions in the basal ganglia, pons, and cerebellum were identified. The number of neurons in the putamen and immunoreactivity for dopamine receptors were well-preserved. In contrast, significant neuronal loss and decreased dopamine receptor immunoreactivity in the putamen were observed in the “levodopa-unresponsive” MSA-P control patients. These putaminal pathology results were consistent with the findings of premortem magnetic resonance imaging (MRI). All three patients similarly exhibited severe neuronal loss in the substantia nigra and decreased immunoreactivity for dopamine transporter.

Conclusion

Levodopa responsiveness in patients with MSA-P may be corroborated by the normal putamen on MRI and the preserved postsynaptic nigrostriatal dopaminergic system on pathological examination. The results presented in this study may provide a rationale for continuation of levodopa treatment in patients diagnosed with MSA-P.
Sugar labeling information and online marketing strategies for hand-shaken tea drinks in northern Taiwan
Authors: Liu Chi-Hsuan , Wong Te-Chih , Chung Mei , Bai Chyi-Huey , Chen Yi-Chun
Published: November 2023

Background

Sugar-sweetened beverages (SSBs) are the main cause of excessive sugar intake and increased health risks. Food companies usually use social media to market SSBs in order to increase consumers’ purchase intentions. To reduce excessive added sugar consumption from hand-shaken tea drinks, Taiwan has implemented a mandatory policy requiring clear sugar content labeling. This study aimed to investigate the sugar label information and online marketing strategies for hand-shaken tea drinks in northern Taiwan.

Methods

In this cross-sectional study, content analysis was employed to investigate the sugar labeling information and the current situation of online marketing in hand-shaken tea drink brands based in northern Taiwan. Seventy-two hand-shaken tea drink brands’ stores were visited to record their sugar labeling presentation methods, with brands lacking labeling, presenting incomplete labeling, or not offering customized sugar levels being excluded, resulting in 60 brands being chosen for the subsequent data collection process. The sugar and energy contents in 1,581 hand-shaken tea drinks were recorded and calculated. Subsequently, the sugar contents were assessed in accordance with World Health Organization (WHO) sugar recommendations (25 g/day), warning label criteria, and Taiwan’s regulations for low-sugar packaged beverages. Seven brands that had high online impressions were further selected and their marketing strategies in 560 Facebook posts were analyzed.

Results

The presentation methods of labeling varied among the 60 brands, and only 42 brands had obvious and easily accessible labeling. The most common labeling presentation method was posters (n = 28). After converting the sugar content of half-sugar and low-sugar hand-shaken tea drinks, it was found that 60.2% of half-sugar beverages and 13.0% of low-sugar beverages exceeded 25 g of sugar per cup. Over 90% of brands had Facebook and Instagram accounts. The top marketing strategies for tea drink brands on Facebook were specific beverage information, brand information, and nutrition and health marketing. Most posts promoted sugar-sweetened beverages.

Conclusion

Not all hand-shaken tea drink brands in this study followed Taiwan’s labeling regulations. Moreover, high sugar contents in hand-shaken tea drinks labeled as half-sugar and low-sugar could potentially lead people to unconsciously consume excessive amounts of sugar. Future research should explore the impact of online marketing strategies on SSBs consumption behavior and ways to mitigate it among the Taiwanese public.
Associations between homocysteine, vitamin B12, and folate and the risk of all-cause mortality in American adults with stroke
Authors: Zhang Panpan , Xie Xia , Zhang Yurong
Published: November 2023

Objective

Associations between plasma homocysteine (Hcy), vitamin B12, and folate and the risk of all-cause mortality are unclear. This study aimed to examine whether plasma Hcy, vitamin B12, and folate levels independently predict the risk of all-cause mortality in American adults with stroke.

Methods

Data from the United States National Health and Examination Survey (NHANES; 1999–2006) were used and linked with the latest (2019) National Death Index (NDI). Cox proportional hazards models and restricted cubic splines were used to estimate the hazard ratios (HR) and 95% confidence intervals (CI) of all-cause mortality for Hcy, folate, and B12 levels in adults with stroke. Sample weights were calculated to ensure the generalizability of the results.

Results

A total of 431 participants were included (average age: 64.8 years). During a median follow-up of 10.4 years, 316 deaths occurred. Hcy was positively associated with all-cause mortality in adults with stroke (HR, 1.053; 95% CI: 1.026–1.080). Stroke patients with plasma Hcy levels in the fourth quartile had a 1.631-fold higher risk of all-cause mortality (HR, 1.631; 95% CI: 1.160–2.291) than those in the first quartile. The association between plasma Hcy and all-cause mortality was strong significant in older patients (p for interaction = 0.020). Plasma folate and vitamin B12 concentrations were inversely correlated with Hcy concentrations [B-value (95% CI): −0.032 (−0.056– −0.008), −0.004 (−0.007– −0.002), respectively]. No significant associations were observed between folate, vitamin B12 levels, and all-cause mortality in adults with stroke.

Conclusion

Plasma Hcy levels were positively associated with all-cause mortality in older adults with stroke. Folate and vitamin B12 levels were inversely correlated with Hcy. Plasma Hcy may serve as a useful predictor in mortality risk assessment and targeted intervention in adults with stroke.
Comprehensive genomic profiling reveals prognostic signatures and insights into the molecular landscape of colorectal cancer
Authors: Yang Jinwei , Zhao Sihui , Su Junyan , Liu Siyao , Wu Zaozao , Ma Wei , Tang Ming , Wu Jingcui , Mao Erdong , Han Li , Liu Mengyuan , Zhang Jiali , Cao Lei , Shao Jingyi , Shang Yun
Published: November 2023

Background

Colorectal cancer (CRC) is a prevalent malignancy with diverse molecular characteristics. The NGS-based approach enhances our comprehension of genomic landscape of CRC and may guide future advancements in precision oncology for CRC patients.

Method

In this research, we conducted an analysis using Next-Generation Sequencing (NGS) on samples collected from 111 individuals who had been diagnosed with CRC. We identified somatic and germline mutations and structural variants across the tumor genomes through comprehensive genomic profiling. Furthermore, we investigated the landscape of driver mutations and their potential clinical implications.

Results

Our findings underscore the intricate heterogeneity of genetic alterations within CRC. Notably, BRAF, ARID2, KMT2C, and GNAQ were associated with CRC prognosis. Patients harboring BRAF, ARID2, or KMT2C mutations exhibited shorter progression-free survival (PFS), whereas those with BRAF, ARID2, or GNAQ mutations experienced worse overall survival (OS). We unveiled 80 co-occurring and three mutually exclusive significant gene pairs, enriched primarily in pathways such as TP53, HIPPO, RTK/RAS, NOTCH, WNT, TGF-Beta, MYC, and PI3K. Notably, co-mutations of BRAF/ALK, BRAF/NOTCH2, BRAF/CREBBP, and BRAF/FAT1 correlated with worse PFS. Furthermore, germline AR mutations were identified in 37 (33.33%) CRC patients, and carriers of these variants displayed diminished PFS and OS. Decreased AR protein expression was observed in cases with AR germline mutations. A four-gene mutation signature was established, incorporating the aforementioned prognostic genes, which emerged as an independent prognostic determinant in CRC via univariate and multivariate Cox regression analyses. Noteworthy BRAF and ARID2 protein expression decreases detected in patients with their respective mutations.

Conclusion

The integration of our analyses furnishes crucial insights into CRC’s molecular characteristics, drug responsiveness, and the construction of a four-gene mutation signature for predicting CRC prognosis.
Medication economic burden of antidepressant non-adherence in Spain
Authors: Gutiérrez-Abejón Eduardo , Pedrosa-Naudín M. Aránzazu , Fernández-Lázaro Diego , Alvarez F. Javier
Published: November 2023

Introduction: Non-adherence to antidepressants is associated with worse disease outcomes (morbidity and mortality) and correlates with higher healthcare resource utilization and costs.

Methods: A population-based registry study was conducted to assess non-adherence and to analyze the economic burden of treatment and from non-adherence to antidepressants in 2021. Non-adherence was measured by the Medication Possession Ratio and those below 80% were classified as non-adherent.

Results: In 2021, 246,718 patients (10.60% [95% CI: 10.48–10.72]) received antidepressants at a cost of €29 million. The median antidepressant cost per patient/year was €70.08€, ranging from €7.58 for amitriptyline to €396.66 for agomelatine. Out-of-pocket costs represented 6.09% of total expenditures, with a median copayment of €2.78 per patient. The 19.87% [95% CI 19.52–20.22)] of patients were non-adherent to antidepressants, costing €3.9 million (13.30% of total antidepressant costs). Non-adherence rates exceeded 20% for the tricyclic antidepressants, fluoxetine (23.53%), fluvoxamine (22.42%), and vortioxetine (20.58%). Venlafaxine (14.64%) and citalopram (14.88%) had the lowest non-adherence rates, of less than 15%. The median cost of non-adherent medications per patient/year was €18.96 and ranged from €2.50 (amitriptyline) to €133.42 (agomelatine).

Conclusion: Reducing non-adherence to antidepressants is critical to improving clinical and economic outcomes. The implementation of interventions and standardized measures, including early detection indicators, is urgently needed. Antidepressants differ with regard to non-adherence and their cost, and this should be considered when prescribing this medication. The Medication Possession Ratio could be used by the healthcare provider and clinician to identify non-adherent patients for monitoring, and to take necessary corrective actions.
Evolution of pharmacogenomic services and implementation of a multi-state pharmacogenomics clinic across a large rural healthcare system
Authors: Van Heukelom Joel , Morgan Jennifer , Massmann Amanda , Jacobsen Kristen , Petry Natasha J. , Baye Jordan F. , Frear Samantha , Schultz April
Published: November 2023

Introduction: Pharmacogenomics (PGx) aims to maximize drug benefits while minimizing risk of toxicity. Although PGx has proven beneficial in many settings, clinical uptake lags. Lack of clinician confidence and limited availability of PGx testing can deter patients from completing PGx testing. A few novel PGx clinic models have been described as a way to incorporate PGx testing into the standard of care.

Background: A PGx clinic was implemented to fill an identified gap in provider availability, confidence, and utilization of PGx across our health system. Through a joint pharmacist and Advanced Practice Provider (APP) collaborative clinic, patients received counseling and PGx medication recommendations both before and after PGx testing. The clinic serves patients both in-person and virtually across four states in the upper Midwest.

Results: The majority of patients seen in the PGx clinic during the early months were clinician referred (77%, n = 102) with the remainder being self-referred. Patients were, on average, taking two medications with Clinical Pharmacogenetics Implementation Consortium guidelines. Visits were split almost equally between in-person and virtual visits.

Conclusion: Herein, we describe the successful implementation of an interdisciplinary PGx clinic to further enhance our PGx program. Throughout the implementation of the PGx clinic we have learned valuable lessons that may be of interest to other implementors. Clinicians were actively engaged in clinic referrals and early adoption of telemedicine was key to the clinic’s early successes.
Resistance to immune checkpoint inhibitors in gastric cancer
Authors: Liu Kai , Yuan Shiman , Wang Chenyu , Zhu Hong
Published: November 2023

Gastric cancer (GC) is one of the most common gastrointestinal malignancies worldwide. In the past decade, with the development of early diagnostic techniques, a clear decline in GC incidence has been observed, but its mortality remains high. The emergence of new immunotherapies such as immune checkpoint inhibitors (ICIs) has changed the treatment of GC patients to some extent. However, only a small number of patients with advanced GC have a durable response to ICI treatment, and the efficacy of ICIs is very limited. Existing studies have shown that the failure of immunotherapy is mainly related to the development of ICI resistance in patients, but the understanding of the resistance mechanism is still insufficient. Therefore, clarifying the mechanism of GC immune resistance is critical to improve its treatment and clinical benefit. In this review, we focus on summarizing the mechanisms of primary or acquired resistance to ICI immunotherapy in GC from both internal and external aspects of the tumor. At the same time, we also briefly discuss some other possible resistance mechanisms in light of current studies.
Wideband hybrid metamaterial absorber via compound design of multiple mechanisms
Authors: Wang Zhenxu , Xiong Xinmeng , Liang Jian-Gang , Wang Jiafu , Fu Xinmin , Ding Chang , Han Yajuan , Sun Yong , Wang Yudeng , Zhou Junxiang , Jiang Juanna , Wang Wenjie , Qu Shaobo
Published: November 2023

Broadband and high efficiency are the two core indexes of absorption research, which usually requires a balance between them. Therefore, how to take into account both and achieve broadband and efficient absorption is a hot topic in current research. In this paper, by the compound design of multiple mechanisms, a kind of wideband hybrid metamaterial absorber (HMA) is proposed. The overall structure consists of a layer of patterned resistive film and a layer of magnetic absorbing material (MAM) separated by the air. The resistive layer is designed as square ring type to regulate the local magnetic field, which results in significant magnetic field enhancement within the MAM layer, and this mechanism provides a prerequisite for wideband and high-efficiency absorption in the low frequency band. Furthermore, due to the electrical losses of the resistive film, another absorption band is additionally excited in the high frequency band. Thanks to the multiple mechanisms, the absorption efficiency above 90% in the 3.2–22.0 GHz frequency band can be realized, and the thickness of the overall structure is 7.0 mm that is 0.07 of the wavelengths at the lowest frequency point. To demonstrate this method, a prototype is designed, fabricated and measured. Both the simulation and experiment results verify the effectiveness of the proposed method. This work provides a new method to design wideband and high-efficiency electromagnetic absorption structures and may find potential applications in multi-functional planar or conformal structures.
Comparing body temperature measurements using the double sensor method within a wearable device with oral and core body temperature measurements using medical grade thermometers—a short report
Authors: Eisenkraft Arik , Goldstein Nir , Fons Meir , Tabi Michael , Sherman Anna Danielle , Ben Ishay Arik , Merin Roei , Nachman Dean
Published: November 2023

Introduction: Body temperature is essential for diagnosing, managing, and following multiple medical conditions. There are several methods and devices to measure body temperature, but most do not allow continuous and prolonged measurement of body temperature. Noninvasive skin temperature sensor combined with a heat flux sensor, also known as the “double sensor” technique, is becoming a valuable and simple method for frequently monitoring body temperature.

Methods: Body temperature measurements using the “double sensor” method in a wearable monitoring device were compared with oral and core body temperature measurements using medical grade thermometers, analyzing data from two prospective clinical trials of different clinical scenarios. One study included 45 hospitalized COVID-19 patients in which oral measurements were taken using a hand-held device, and the second included 18 post-cardiac surgery patients in which rectal measurements were taken using a rectal probe.

Results: In study 1, Bland-Altman analysis showed a bias of −0.04°C [0.34–(−0.43)°C, 95% LOA] with a correlation of 99.4% (p < 0.001). In study 2, Bland-Altman analysis showed a bias of 0.0°C [0.27–(−0.28)°C, 95% LOA], and the correlation was 99.3% (p < 0.001). In both studies, stratifying patients based on BMI and skin tone showed high accordance in all sub-groups.

Discussion: The wearable monitor showed high correlation with oral and core body temperature measurements in different clinical scenarios.
Generation of high oleic acid sunflower lines using gamma radiation mutagenesis and high-throughput fatty acid profiling
Authors: Rozhon Wilfried , Ramirez Veronica E. , Wieckhorst Silke , Hahn Volker , Poppenberger Brigitte
Published: November 2023

Sunflower (Helianthus annuus L.) is the second most important oil seed crop in Europe. The seeds are used as confection seeds and, more importantly, to generate an edible vegetable oil, which in normal varieties is rich in the polyunsaturated fatty acid linoleic acid. Linoleic acid is biosynthesized from oleic acid through activity of the oleate desaturase FATTY ACID DESATURASE 2 (FAD2), which in seeds is encoded by FAD2-1, a gene that’s present in single copy in sunflowers. Defective FAD2-1 expression enriches oleic acid, yielding the high oleic (HO) acid trait, which is of great interest in oil seed crops, since HO oil bears benefits for both food and non-food applications. Chemical mutagenesis has previously been used to generate sunflower mutants with reduced FAD2-1 expression and here it was aimed to produce further genetic material in which FAD2-1 activity is lost and the HO trait is stably expressed. For this purpose, a sunflower mutant population was created using gamma irradiation and screened for fad2-1 mutants with a newly developed HPLC-based fatty-acid profiling system that’s suitable for high-throughput analyses. With this approach fad2-1 knock-out mutants could be isolated, which stably hyper-accumulate oleic acid in concentrations of 85-90% of the total fatty acid pool. The genetic nature of these new sunflower lines was characterized and will facilitate marker development, for the rapid introgression of the trait into elite sunflower breeding material.
UAV-based individual plant detection and geometric parameter extraction in vineyards
Authors: Cantürk Meltem , Zabawa Laura , Pavlic Diana , Dreier Ansgar , Klingbeil Lasse , Kuhlmann Heiner
Published: November 2023

Accurately characterizing vineyard parameters is crucial for precise vineyard management and breeding purposes. Various macroscopic vineyard parameters are required to make informed management decisions, such as pesticide application, defoliation strategies, and determining optimal sugar content in each berry by assessing biomass. In this paper, we present a novel approach that utilizes point cloud data to detect trunk positions and extract macroscopic vineyard characteristics, including plant height, canopy width, and canopy volume. Our approach relies solely on geometric features and is compatible with different training systems and data collected using various 3D sensors. To evaluate the effectiveness and robustness of our proposed approach, we conducted extensive experiments on multiple grapevine rows trained in two different systems. Our method provides more comprehensive canopy characteristics than traditional manual measurements, which are not representative throughout the row. The experimental results demonstrate the accuracy and efficiency of our method in extracting vital macroscopic vineyard characteristics, providing valuable insights for yield monitoring, grape quality optimization, and strategic interventions to enhance vineyard productivity and sustainability.
Regulation of secondary growth by poplar BLADE-ON-PETIOLE genes in Arabidopsis
Authors: Li Sibei , Devi Bhaswati , Allam Gamalat , Bhullar Armaan , Murmu Jhadeswar , Li Eryang , Hepworth Shelley R.
Published: November 2023

BLADE-ON-PETIOLE (BOP) genes are essential regulators of vegetative and reproductive development in land plants. First characterized in Arabidopsis thaliana (Arabidopsis), members of this clade function as transcriptional co-activators by recruiting TGACG-motif binding (TGA) basic leucine zipper (bZIP) transcription factors. Highly expressed at organ boundaries, these genes are also expressed in vascular tissue and contribute to lignin biosynthesis during secondary growth. How these genes function in trees, which undergo extensive secondary growth to produce wood, remains unclear. Here, we investigate the functional conservation of BOP orthologs in Populus trichocarpa (poplar), a widely-used model for tree development. Within the poplar genome, we identified two BOP-like genes, PtrBPL1 and PtrBPL2, with abundant transcripts in stems. To assess their functions, we used heterologous assays in Arabidopsis plants. The promoters of PtrBPL1 and PtrBPL2, fused with a β-glucuronidase (GUS) reporter gene showed activity at organ boundaries and in secondary xylem and phloem. When introduced into Arabidopsis plants, PtrBPL1 and PtrBPL2 complemented leaf and flower patterning defects in bop1 bop2 mutants. Notably, Arabidopsis plants overexpressing PtrBPL1 and PtrBPL2 showed defects in stem elongation and the lignification of secondary tissues in the hypocotyl and stem. Finally, PtrBPL1 and PtrBPL2 formed complexes with TGA bZIP proteins in yeast. Collectively, our findings suggest that PtrBPL1 and PtrBPL2 are orthologs of Arabidopsis BOP1 and BOP2, potentially contributing to secondary growth regulation in poplar trees. This work provides a foundation for functional studies in trees.
Protection against insect predation during fruit development: the role of fleshy fruit wings of three species of Zygophyllum in the cold desert of Central Asia
Authors: Xie Kaiqing , Qiu Juan , Mamut Jannathan , Li Yuting , Tan Dunyan
Published: November 2023

Introduction

Fruit wings serve various ecological functions, including facilitating wind dispersal, providing physical protection to seeds, and regulating seed germination. While many studies have reported the role of fruit wings in plants, little is known about their protective function during fruit development.

Methods

In this study, winged fruits damaged by insects in natural populations of three Zygophyllum species (Z. potaninii, Z. lehmannianum and Z. macropterum) were investigated. We measured and compared the percentage of damaged winged fruits, seed set, seed mass, seed germination, and seedling growth of different insect herbivory categories.

Results

The results revealed that the percentage of winged fruits with damaged wings only (low predation) was significantly higher than that of with damaged both fruit wings and fruit bodies (high predation). Furthermore, winged fruits with low predation had significant higher seed set, seed mass, seed germination, and dry mass and relative growth rate (RGR) in the seedlings which grown from the seeds, than that from winged fruits with high predation.

Discussion

These results demonstrate that the presence of the fruit wings may provide protection for the seeds to alleviate harm from insect predation before dispersal. These findings provide new insights into the function of fruit wings and the reproductive strategies of desert plants.
Napier grass (Pennisetum purpureum Schum) management strategies for dairy and meat production in the tropics and subtropics: yield and nutritive value
Authors: Islam M. Rafiq , Garcia Sergio C. , Sarker Nathu R. , Islam Md. Ashraful , Clark Cameron E. F.
Published: November 2023

Napier grass (Pennisetum purpureum Schumach) comprises up to 80% of the cattle diet in many tropical and subtropical regions and is used primarily by smallholder farmers. Despite the grass’s high yield, resulting animal productivity from this grass is low. One of the key reasons for the low animal productivity of Napier grass is its low nutritive value under current management. Taken together, previous work has shown the current yield, crude protein (CP), and metabolisable energy (ME) of Napier grass to be 26 t dry matter (DM)/ha/year, 96 g/kg DM, and 8.7 MJ/kg DM, respectively, ranging from 2 to 86 t DM/ha/year, 9 to 257 g CP/kg DM, and 5.9 to 10.8 MJ ME/kg DM, respectively, suggesting an opportunity for significant improvement on both yield and nutritive value of this grass. The DM yield and nutritive value of this grass are inversely related, indicating a trade-off between yield and quality; however, this trade-off could be minimised by increasing sowing density and harvesting frequency. Available literature shows that this simple management strategy of increasing sowing density (50 cm × 40 cm) and harvesting frequency (11–12 harvests/year) provides 71 t DM/ha with 135 g/kg DM CP and 10.8 MJ ME/kg DM. This quality of Napier grass has the potential to increase both milk and meat production substantially in the tropics and subtropics, and the farmers will likely find this simple management acceptable due to the high yield obtained through this management. However, there is a paucity of work in this field. Therefore, management strategies to improve the nutritive value of Napier grass are required to increase milk and meat production in the tropics and subtropics and in doing so improve the food security of more than half of the global population living in these regions.
A comparative analysis of morphology, microstructure, and volatile metabolomics of leaves at varied developmental stages in Ainaxiang (Blumea balsamifera (Linn.) DC.)
Authors: Chen Xiaolu , Li Yanqun , Pang Yuxin , Shen Wanyun , Chen Qilei , Liu Liwei , Luo Xueting , Chen Zhenxia , Li Xingfei , Li Yulan , Zhang Yingying , Huang Mei , Yuan Chao , Wang Dan , Guan Lingliang , Liu Yuchen , Yang Quan , Chen Hubiao , Wu Hong , Yu Fulai
Published: November 2023

Introduction

Ainaxiang (Blumea balsamifera (Linn.) DC.) is cultivated for the extraction of (-)-borneol and other pharmaceutical raw materials due to its abundant volatile oil. However, there is limited knowledge regarding the structural basis and composition of volatile oil accumulation in fresh B. balsamifera leaves.

Methods

To address this problem, we compare the fresh leaves’ morphology, microstructure, and volatile metabonomic at different development stages, orderly defined from the recently unfolded young stage (S1) to the senescent stage (S4).

Results and discussion

Distinct differences were observed in the macro-appearance and microstructure at each stage, particularly in the B. balsamifera glandular trichomes (BbGTs) distribution. This specialized structure may be responsible for the accumulation of volatile matter. 213 metabolites were identified through metabolomic analysis, which exhibited spatiotemporal accumulation patterns among different stages. Notably, (-)-borneol was enriched at S1, while 10 key odor metabolites associated with the characteristic balsamic, borneol, fresh, and camphor aromas of B. balsamifera were enriched in S1 and S2. Ultra-microstructural examination revealed the involvement of chloroplasts, mitochondria, endoplasmic reticulum, and vacuoles in the synthesizing, transporting, and storing essential oils. These findings confirm that BbGTs serve as the secretory structures in B. balsamifera, with the population and morphology of BbGTs potentially serving as biomarkers for (-)-borneol accumulation. Overall, young B. balsamifera leaves with dense BbGTs represent a rich (-)-borneol source, while mesophyll cells contribute to volatile oil accumulation. These findings reveal the essential oil accumulation characteristics in B. balsamifera, providing a foundation for further understanding.
Content and process: organizational conflict and decision making
Authors: Gaba Vibha , Joseph John
Published: November 2023

The foundational work in the Carnegie perspective established conflict as endemic to organizations and a driver of organizing behavior and decision making. Organizations as a system of coordinated action among interdependent individuals and groups with different preferences, interests, information, or knowledge create the potential for pervasive and ongoing latent goal conflict. At the same time, extant psychology research has devoted considerable attention to identifying the content and intensity of conflict, focusing on the relationship between different types of conflict and their impact on group outcomes. The Carnegie perspective also assumes that the need for joint decision-making and the differences in goals or perception of reality are never fully resolved. As a result, it has paid attention to the processes through which conflict is addressed - by attending sequentially to goals, decentralizing information, accumulating excess resources, and forming coalitions rather than formal mediating procedures. The assessment of the psychology and organizational theory research also suggests that future work focusing on organizational conflict as latent, situated, and dynamic would enable greater clarity on how organizations make decisions.
Attachment anxiety and cyberbullying victimization in college students: the mediating role of social media self-disclosure and the moderating role of gender
Authors: Yang Xixi , Huang Yitong , Li Benqian
Published: November 2023

Backgrounds and purpose

Cyberbullying is a globally prevalent social problem that threatens the wellbeing of young people. Despite a rising call for more research focused on cyberbullying victims, our understanding of the psychological and behavioral risk factors associated with cyberbullying victimization (CV) remains limited, especially among the Chinese population. However, such information is crucial for identifying potential victims and planning targeted educational and protective interventions. In this paper, we report an empirical investigation into how attachment anxiety (AA), social media self-disclosure (SMSD), and gender interplay with each other to influence CV.

Methods

Cross-sectional survey data from 845 Chinese college students (Female = 635, Mage = 18.7) were analyzed in SPSS PROCESS using Haye’s macro with the bootstrap method.

Results

Our data support a moderated mediation model. First, SMSD partially mediates the positive relationship between AA and CV, which suggests individuals with high AA tend to engage in risky and excessive self-disclosure behavior on social media, which, in turn, expose them to an increased risk of cyberbullying. Second, gender moderates the direct AA-CV path and the second stage of the mediation path, making the effect of AA on CV appear more direct in males (i.e., not mediated by SMSD) and more indirect (i.e., fully mediated through SMSD) in females.

Conclusion

The results contribute to an ongoing endeavor to better understand the psychological and behavioral mechanisms underlying CV and develop effective strategies to identify and protect vulnerable individuals.
Neutrophil to lymphocyte ratio, platelet to lymphocyte ratio, and monocyte to lymphocyte ratio in ADHD: a systematic review and meta-analysis
Authors: Gędek Adam , Modrzejewski Szymon , Gędek Marta , Antosik Anna Z. , Mierzejewski Paweł , Dominiak Monika
Published: November 2023

Introduction

Attention-deficit hyperactivity disorder (ADHD) is a neurodevelopmental disorder with an unclear etiology. Systemic inflammation and immune dysregulation may play a role in the pathogenesis of ADHD. Morphology-derived parameters such as neutrophil to lymphocyte ratio (NLR), platelet to lymphocyte ratio (PLR), and monocyte to lymphocyte ratio (MLR), have been proposed as peripheral biomarkers of the immune-inflammatory process in various diseases. However, studies examining their role in ADHD remain inconclusive.

Methods

A systematic review and a meta-analysis were conducted to evaluate the association between NLR, MLR, PLR and ADHD. Relevant articles were identified, screened, and assessed for quality according to PRISMA guidelines. Moreover, a qualitative and quantitative analyses were performed.

Results

The review contained eight eligible studies, five of which were included in the meta-analysis. The meta-analysis showed that ADHD patients had higher NLR and PLR values compared to health controls. No significant difference in MLR value was observed between the two groups. Analysis in relation to ADHD subtypes showed no significant differences in inflammatory markers in any of the included studies as well. The influence of medical treatment on these ratios could not be adequately assessed due to limited data.

Conclusion

ADHD patients exhibit higher NLR and PLR than healthy controls, which may indicate the potential immune-inflammatory involvement in this disorder. Further studies on inflammatory markers and ADHD, especially those considering the impact of treatment and clinical symptoms, are essential to comprehensively understand this association.
Utilizing artificial intelligence-based eye tracking technology for screening ADHD symptoms in children
Authors: Chen Xiaolu , Wang Sihan , Yang Xiaowen , Yu Chunmei , Ni Fang , Yang Jie , Tian Yu , Ye Jiucai , Liu Hao , Luo Rong
Published: November 2023

Objective

To explore the potential of using artificial intelligence (AI)-based eye tracking technology on a tablet for screening Attention-deficit/hyperactivity disorder (ADHD) symptoms in children.

Methods

We recruited 112 children diagnosed with ADHD (ADHD group; mean age: 9.40 ± 1.70 years old) and 325 typically developing children (TD group; mean age: 9.45 ± 1.59 years old). We designed a data-driven end-to-end convolutional neural network appearance-based model to predict eye gaze to permit eye-tracking under low resolution and sampling rates. The participants then completed the eye tracking task on a tablet, which consisted of a simple fixation task as well as 14 prosaccade (looking toward target) and 14 antisaccade (looking away from target) trials, measuring attention and inhibition, respectively.

Results

Two-way MANOVA analyses demonstrated that diagnosis and age had significant effects on performance on the fixation task [diagnosis: F(2, 432) = 8.231, ***p < 0.001; Wilks’ Λ = 0.963; age: F(2, 432) = 3.999, *p < 0.019; Wilks’ Λ = 0.982], prosaccade task [age: F(16, 418) = 3.847, ***p < 0.001; Wilks’ Λ = 0.872], and antisaccade task [diagnosis: F(16, 418) = 1.738, *p = 0.038; Wilks’ Λ = 0.938; age: F(16, 418) = 4.508, ***p < 0.001; Wilks’ Λ = 0.853]. Correlational analyses revealed that participants with higher SNAP-IV score were more likely to have shorter fixation duration and more fixation intervals (r = −0.160, 95% CI [0.250, 0.067], ***p < 0.001), poorer scores on adjusted prosaccade accuracy, and poorer scores on antisaccade accuracy (Accuracy: r = −0.105, 95% CI [−0.197, −0.011], *p = 0.029; Adjusted accuracy: r = −0.108, 95% CI [−0.200, −0.015], *p = 0.024).

Conclusion

Our AI-based eye tracking technology implemented on a tablet could reliably discriminate eye movements of the TD group and the ADHD group, providing a potential solution for ADHD screening outside of clinical settings.
Social media addiction as a mediator of the associations between fear of COVID-19, mental health symptoms, and problematic alcohol use
Authors: Luk Jeremy W. , Geda Daniel W. , Stangl Bethany L. , Cheng Cecilia , Schwandt Melanie L. , Goldman David , Diazgranados Nancy , Ramchandani Vijay A.
Published: November 2023

Background

Fear of COVID-19 is a risk factor for anxiety and depressive symptoms. During the COVID-19 pandemic, drinking to cope with psychological distress has been proposed as a key mechanism leading to problematic drinking. The goal of this study was to test social media addiction as a mediator linking fear of COVID-19 to mental health symptoms and problematic alcohol use.

Methods

In between April 6 and July 2 of 2022, 250 participants completed an online survey as part of the National Institute on Alcohol Abuse and Alcoholism COVID-19 Pandemic Impact on Alcohol Study. Path analyses were conducted to test the mediational pathways.

Results

Using the polythetic classification scheme, 13.2% (n = 33) of participants were classified as having social media addiction. Compared with participants without social media addiction, participants with social media addiction spent significantly more time on social media platforms and on digital communications with a family member or friend. They also reported greater fear of COVID-19, higher anxiety symptoms, and higher depressive symptoms. Path analyses indicated that social media addiction mediated the associations of fear of COVID-19 with anxiety and depressive symptoms. Furthermore, there were indirect pathways linking fear of COVID-19 to problematic alcohol use through higher social media addiction and higher anxiety and depressive symptoms.

Conclusion

Social media addiction may be a maladaptive coping mechanism that individuals with high fear of COVID-19 utilized to deal with uncertainty and perceived risks during the pandemic. Findings underscore the need to examine cognitions related to fear of COVID-19 and address excessive social media use in the context of mental health and alcohol interventions.
Longitudinal study on blood and biochemical indexes of Tibetan and Han in high altitude area
Authors: Yuan ZhiMin , Zou YuanWu , Liu XiaoXing , Wang LongHao , Chen Cheng
Published: November 2023

Objective

This study aims to review the blood routine and biochemical indicators of the plateau population for three consecutive years, and analyze the impact of the plateau on these blood indicators of the Tibetan population and the Han immigrant population.

Method

These parameters were extracted from the Laboratory Department of Ali District People’s Hospital in Tibet from January 2019 to December 2021, including blood routine, liver and kidney function, blood lipids, myocardial enzyme spectrum, and rheumatic factor indicators. Changes in these parameters were analyzed over 3 consecutive years according to inclusion and exclusion criteria.

Result

A total of 114 Tibetans and 93 Hans participated in the study. These parameters were significantly different between Tibetan and Han populations. Red blood cells (RBC), hemoglobin (HGB), hematocrit (HCT), mean hemoglobin content (MCH), mean corpuscular hemoglobin concentration (MCHC), white blood cells (WBC), lymphocytes (LYMPH) and monocytes (MONO) were significantly higher in Hans than Tibetans (p < 0.05). Biochemically, total bilirubin (TBIL), direct bilirubin (DBIL), albumin (ALB), urea nitrogen (Urea), creatinine (Cr), uric acid (UA), glucose (GLU), triglycerides (TG) and creatine kinase isoenzyme (CKMB) were significantly higher in Hans than Tibetans; aspartate aminotransferase (AST), glutamyl transpeptidase (GGT), alkaline phosphatase (ALP), antistreptolysin (ASO), and C-reactive protein (CRP) were significantly higher in Tibetans than Hans (p < 0.05). There were no obvious continuous upward or downward trend of the parameters for 3 consecutive years.

Conclusion

In high-altitude areas, Han immigrants have long-term stress changes compared with Tibetans. The main differences are reflected in the blood system, liver and kidney functions, etc., which provide basic data for further research on the health status of plateau populations.
Learning with privileged and sensitive information: a gradient-boosting approach
Authors: Yan Siwen , Odom Phillip , Pasunuri Rahul , Kersting Kristian , Natarajan Sriraam
Published: November 2023

We consider the problem of learning with sensitive features under the privileged information setting where the goal is to learn a classifier that uses features not available (or too sensitive to collect) at test/deployment time to learn a better model at training time. We focus on tree-based learners, specifically gradient-boosted decision trees for learning with privileged information. Our methods use privileged features as knowledge to guide the algorithm when learning from fully observed (usable) features. We derive the theory, empirically validate the effectiveness of our algorithms, and verify them on standard fairness metrics.