bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect Of Profitability, Leverage And Accounting Contributions On The Disclosure Of Sustainability Reports In Mining Companies Listed On The Indonesia Stock Exchange For The 2020-2022 Period
Authors: Maliska Tasya , Wiyodo Slamet
Published: January 2025

This research was conducted with the aim of knowing the effect of Profitability, Leverage, and Liquidity on the disclosure of Sustainability Reporting in mining companies listed on the Indonesia Stock Exchange for the 2020-2022 period. The sample selection method used in this study was purposive sampling in order to obtain a sample of 35 companies that met the criteria. The analysis technique used in this research is multiple linear regression analysis technique. The results in this study indicate that Profitability has a positive effect on the disclosure of Sustainability Reporting in Mining companies listed on the Indonesia Stock Exchange for the 2020-2022 period, while Leverage and Liquidity have no effect on the disclosure of Sustainability Reporting in Mining companies listed on the Indonesia Stock Exchange for the 2020-2022 period.
The Socio-Economic Context of Folk Artisans in Bihar: An Exploration of their Livelihood and Challenges
Authors:   Jawaharlal Nehru University, Priyadarshi Anupam
Published: January 2025

This research paper delved into the complex relationship among the socioeconomic lives of Nachaniya1i.e. folk emotioneers of Kabir-Lila2, Raja Salhes3, Reshma Chuharmal4 and Dina-Bhadri5 in Bihar. By examining the lives of these emotioneers who keep and perform conventional folk performances, the observe goals to apprehend the situations and possibilities they face in the society. Meanwhile, these emotioneers belonged to the marginalized community, offered a very specific perspective on their performances. Performances often performed community narratives of socio-religious harmony, inter-caste love, and anti-feudal struggle serving as powerful symbols of resistance and resilience for marginalized community. These tales undertaking societal norms and inspire desire, contributing to a collective identity, so that groups continued their performances in the society. Besides analysing their folk performances, this paper meanwhile looked into the socioeconomic dimensions of folk emotioneers, which insights into their individual social status and the elements shaping their social life. The data gathered from 2015 to 2017 from their performances at Gangaur, in the district of Madhubani; bordering areas of Nepal and Bihar. These emotioneers mostly belonged to marginalised community involved in the Naach groups. They had a low level of income and performing in the Naach groups were one of the additional sources of income. Through analysing the socioeconomic lives of these emotioneers; the study targeted to apprehend the difficult interface among folk practices, social identities, and economic conditions. The study focused in the diverse socioeconomic backgrounds of these artists, exploring the associated factors along with caste, schooling, and earnings assets.
MAGNETIC RESONANCE IMAGING OF LUMBAR SPINE IN ELDERLY PATIENTS OF FAISALABAD DIVISION: AGE RELATED CHANGES AND PATHOLOGIES
Authors: Tariq Kainat , Tasra Bibi  , Moazma Aijaz  , Amna sana  , Wazooha Habib  , Ammara Riaz
Published: January 2025

Background: Spinal disorders are increasingly prevalent in aging populations, significantly impacting mobility, functional independence, and overall quality of life. Magnetic resonance imaging (MRI) serves as a valuable tool in diagnosing and assessing degenerative and pathological spinal conditions without exposing patients to ionizing radiation. This study aimed to explore the epidemiology of lumbar spine disorders using MRI, identify their prevalence and severity, and provide insights to aid clinical management and improve patient outcomes.
Objective: To evaluate the prevalence, severity, and associated factors of lumbar spine disorders among older adults using MRI.
Methods: This cross-sectional study was conducted at Allied Hospital Faisalabad over six months, involving 120 patients who underwent lumbar spine MRI. Participants were selected using stratified random sampling and categorized into four age groups: 41–50 years (38.3%), 51–60 years (29.2%), 61–70 years (20.8%), and over 70 years (11.7%). A standardized questionnaire collected data on demographics, clinical history, and MRI findings. MRI scans were performed on a 1.5 Tesla machine, and data were analyzed using SPSS version 25. Descriptive statistics summarized findings, and Chi-square tests were applied to assess age- and gender-based variations.
Results: The cohort included 82 males (68.3%) and 38 females (31.7%) with a mean age of 55.15 ± 10.63 years. Back pain was reported by 64.2% of patients, trauma by 21.7%, spinal surgery by 17.5%, and osteoporosis by 40.8%. Facet joint osteoarthritis was present in 11.7% (mild), 6.7% (moderate), and 4.2% (severe), while 77.5% showed no signs of osteoarthritis. Lumbar spinal stenosis was observed in 62.5% of patients, categorized as mild (20.8%), moderate (34.2%), or severe (7.5%). Vertebral fractures occurred in 17.5% of patients, and other pathologies were present in 26.7%.
Conclusion: The study highlights the high prevalence of lumbar spine disorders, particularly lumbar spinal stenosis and facet joint osteoarthritis, in older adults. These conditions, closely associated with back pain, osteoporosis, and trauma, emphasize the importance of early diagnosis through MRI. Tailored treatment strategies can improve mobility and quality of life in aging populations.
Evaluación del sistema de gestión de la calidad basado en la norma ISO 9001:2015 en laboratorios de micología clínica en Caracas, Venezuela
Authors: Panizo María Mercedes , Bustamante Yacelli , Ferrara Giuseppe Antonio
Published: January 2025

El objetivo de este estudio fue diagnosticar la conformidad del sistema de gestión de calidad (SGC) de los laboratorios de micología clínica con los requisitos de la norma ISO 9001:2015, para conocer su situación actual frente a la aplicación de esta norma. Se planteó un diseño de campo transversal con enfoque cualitativo, con una muestra de tres laboratorios de micología, dos privados y uno público, mediante muestreo no probabilístico por conveniencia. La recolección de los datos se realizó mediante cuestionario y lista de verificación entre diciembre 2018 y enero 2019. Los dos laboratorios privados presentaron conformidad con la norma, mientras que el público se encontraba en proceso de implementación del SGC. El nivel de conocimiento sobre la norma ISO 9001:2015 del personal profesional de los laboratorios de micología clínica fue muy bueno, y existió una buena correlación entre la percepción de la conformidad de la norma por el personal profesional y la conformidad de la misma en el laboratorio donde se desempeña. La norma ISO 9001:2015 puede ser adoptada por los laboratorios de micología clínica como punto de partida para desarrollar su SGC particular, adaptado a sus necesidades funcionales y operativas.
BIORIESGOS EN LABORATORIOS CLÍNICOS CERTIFICADOS BAJO LA NORMA ISO 9001:2015 EN VENEZUELA
Authors: Rangel Hellen
Published: January 2025

En la actualidad, cuando el mundo ha tenido emergencias causadas por eventos epidemiológicos (Zika, Ébola, Cólera, Paludismo, SIDA, COVID19) relacionados con agentes biológicos, es en el sistema sanitario, el de mayor exposición a riesgos biológicos o bioriesgos, al prestar servicios a pacientes que pudieran estar contagiados, lo que genera exposición a infecciones asociadas a bacterias, hongos, virus o parásitos. Son los laboratorios clínicos, algunos, de los más expuestos del sector salud, debido a la complejidad de los procesos preanaliticos, analíticos y postanalíticos. Objetivo: Identificar los bioriesgos en los laboratorios clínicos certificados bajo la Norma ISO 9001:2015 en Venezuela en su fuente, evento y consecuencia. Métodos: La muestra estuvo formada por cuatro (4) de los cinco (5) laboratorios clínicos actualmente certificados bajo la norma ISO 9001. Se realizaron entrevistas, revisión documental y aplicación de cuestionario a los laboratorios participantes, lo que permitió la identificación de los bioriesgos. Resultados y Discusión: Los laboratorios tienen similitudes en cuanto al número de sus colaboradores, sus macroprocesos, tienen certificados ISO 9001 del año 2022 en adelante y tienen previamente contemplado bioriesgos en la extracción y manipulación de muestras, por pinchazos con agujas contaminadas o derrames. Solo un laboratorio contempló el bioriesgo de la disposición final de desechos biológicos. En ninguno de los laboratorios se tiene identificado al personal de recepción como expuesto a bioriesgos, aun cuando ellos interactúan con el paciente, tampoco se tiene contemplado el posible contagio por la ingesta de agua contaminada (botellones). Conclusiones: Al implementar la norma ISO 9001, los laboratorios tienen como requisito desarrollar el pensamiento basado en riesgos. La identificación de riesgos debe ser dinámica, consiente y de seguimiento constante para seguir nutriéndose en el tiempo lo que permite desarrollar una cultura preventiva en vez de reactiva. Los resultados sugieren que la identificación de bioriesgos debe ser más exhaustiva en los procesos en pro de la mejora continua.
Concordance of Grey Scale Findings of Morbidly Adherent Placenta with Intraoperative Findings
Authors: Rabia Qasim  , Abeera Choudry  , Shumaila Khawaja Khail  , Amna Fareed  , Ghazala Huma
Published: January 2025

Objective: To evaluate the concordance between grey scale ultrasound findings and intraoperative observations in cases of morbidly adherent placenta (MAP) to assess the diagnostic accuracy of grey scale imaging in identifying varying degrees of placental adherence.
Methodology: A prospective observational study was conducted at the Department of Obstetrics & Gynecology, PEMH Rawalpindi, from June 2023 to December 2023. Pregnant women aged >18 years, diagnosed with morbidly adherent placenta based on grey scale ultrasound findings, and scheduled for cesarean delivery were included. Intraoperative findings, particularly the extent of placental invasion into the myometrium and surrounding structures, stage of placental attachment, and involvement of the bladder or other organs, were recorded by the attending obstetrician/gynecologist during surgery. The findings were then compared with grey scale imaging results to evaluate diagnostic accuracy. Data was analyzed using SPSS version 26.
Results: The mean age of the participants was 34.78 years, with 91.3% being housewives. Ultrasound findings indicated that Grade 2 placenta lacunae were commonly associated with cases without bladder invasion, whereas Grade 3 lacunae were strongly linked to bladder invasion. The uterine serosa-bladder interface showed no association with morbidly adherent placenta overall but was correlated with bladder invasion specifically. Placental thickness >4 cm was significantly associated with both bladder and broad ligament invasion. Grey scale parameters, including Grade 3 placenta lacunae (PPV: 91.67%), uterine serosa-bladder interface (PPV: 89.29%), and placental thickness >4 cm (PPV: 87.50%), demonstrated high positive predictive values for identifying true cases of morbidly adherent placenta. However, the sensitivity, specificity, and overall diagnostic accuracy of these parameters ranged from moderate to low.
Conclusion: Grey scale ultrasound demonstrates a reliable positive predictive value for diagnosing morbidly adherent placenta, particularly through parameters such as Grade 3 placenta lacunae, uterine serosa-bladder interface, and placental thickness >4 cm. However, its moderate sensitivity, low specificity, and limited overall accuracy underscore the need for complementary diagnostic tools to enhance the accurate identification and management of MAP.
Actualization of Public Service Principles by the Ombudsman of the Republic of Indonesia
Authors: Habibullah Abd Wachid , Rahayu Karindra Yusnita Puji , Tsabita Ananda Tsany , Aini Diyanah Artu , Marantika Dewi
Published: January 2025

The Ombudsman is an independent institution where in carrying out its duties and authorities the Ombudsman runs independently and there is no interference from other institutions or agencies. In this article, we use qualitative normative methods to present data critically and descriptively based on legal norms, legal theories, and legal doctrines regarding the Ombudsman. Using this method, we will present transparently the extent to which the ombudsman is effective in carrying out his functions and duties, especially in handling administration based on the principles of public service, of course, related to the General Principles of Good Government. With the results of the research we conduct, it can provide an overview of the existence of the Ombudsman whether it is optimal and significant for handling and improving the quality of public services with several challenges faced in it such as human resources and compliance from government agencies. With some of the things that we will describe, we will be able to underline the importance of the role and also the existence of the Ombudsman in serving in the community in accordance with the General Principles of Good Government.
Ethical Supervision of Judges to Improve the Integrity of the Constitutional Court
Authors: Rozana Sukma Dewi  , Avien Zakaria  , Aghnia Safana Ilmi
Published: January 2025

This study aims to analyze the juridical implications of the Constitutional Court Decision Number 90/PUU-XXI/2023 on the independence of Constitutional Court judges. To examine the urgency of regulating the ethical supervision of Constitutional Court judges. This research is expected to be material for improving the law related to the supervision of the ethics of the Constitutional Court judges. The type of research is normative legal research. The approach methods used are the types of statutory approaches, case approaches, and conceptual approaches. The technique of collecting legal materials used is a literature study. The data analysis method used is qualitative descriptive. The deductive method of concluding is used. The results of this study show that the juridical implications of the Constitutional Court Decision Number: 90/PUU-XXI/2023 on the independence of Constitutional Court judges are defects that lead to violations of the code of ethics by Constitutional Court judges during the trial process of the decision, resulting in the loss of the independence of Constitutional Court judges, and the issuance of 4 MKMK decisions. This signals that supervision in judicial institutions such as the Constitutional Court has not been optimal. The urgency of regulating the supervision of the ethics of constitutional court judges is that internal supervision in judicial institutions such as the Constitutional Court has not been optimal. Therefore, it is necessary to reorganize the supervision system and institutional management of the Constitutional Court both internally and externally.
Saúde da Família: Integrando Tecnologia, Humanização e Cuidado Integral para um Futuro mais Saudável
Authors: Sobreira Eline Nogueira Santos
Published: January 2025

É com imensa satisfação que apresentamos o livro "Saúde da Família: Integrando Tecnologia, Humanização e Cuidado Integral para um Futuro mais Saudável". Esta obra surge como um marco no entendimento e na aplicação do modelo de Saúde da Família, destacando-se como um alicerce estratégico para a construção de um sistema de saúde mais equitativo, eficiente e centrado nas pessoas. Neste livro, os autores exploram as interseções entre tecnologia, humanização e cuidado integral, abordando como esses elementos se complementam para fortalecer a atenção primária à saúde. A obra enfatiza a importância de integrar inovações tecnológicas aos valores humanos e à prática clínica, criando soluções que ampliem o acesso, melhorem a qualidade do atendimento e promovam a autonomia das equipes de saúde e das comunidades atendidas
PENGARUH GAYA KEPEMIMPINAN DAN MOTIVASI KERJA TERHADAP KINERJA KARYAWAN PADA MADRASAH IBTIDAIYAH IBNU AQIL BOGOR
Authors: Yuliawan Arif , Hendriana Asep
Published: January 2025

Penelitian ini bertujuan untuk mengetahui seberapa besar pengaruh gaya kepemimpinan dan motivasi kerja terhadap kinerja karyawan pada Madrasah Ibtidaiyah Ibnu Aqil Bogor. Penelitian ini dilakukan di Madrasah Ibtidaiyah Ibnu Aqil Bogor Bogor. Penelitian ini menggunakan pendekatan kuantitatif. Teknik pengambilan sampel yaitu metode sampling jenuh pada penelitian ini adalah seluruh karyawan pada Madrasah Ibtidaiyah Ibnu Aqil Bogor yang berjumlah 35 orang (semua populasi dijadikan sampel). Teknik pengumpulan data menngunakan observasi dalam bentuk kuesioner. Teknik pengolahan data yang dilakukan pada penelitian ini adalah uji validitas, uji reliabilitas, uji regresi linier berganda, koefisien determinasi dan uji simultan. Variabel Gaya Kepemimpinan (X1), Motivasi Kerja (X2) dan Kinerja Karyawan (Y), hasil uji validitas mempunyai nilai CICT lebih besar dari 0,3. Hasil penelitian adalah tingkat signifikan Pvalue (0,000) < 0,05 yang artinya ada pengaruh antara gaya kepemimpinan terhadap kinerja karyawan yaitu sebesar 18,4 %. Sedangkan untuk motivasi kerja terhadap kinerja karyawan juga berpengaruh sebesar 77,0 % yang artinya ada pengaruh antara gaya kepemimpinan dan motivasi kerja yaitu sebesar 87,0 %, sedangkan sisanya 13,0 % dipengaruhi oleh variabel lain yang tidak diteliti dalam penelitian ini.
PENGARUH GAYA KEPEMIMPINAN KEPALA SEKOLAH SD DAN BUDAYA KERJA TERHADAP KEPUASAN GURU SD PADA SCHOOL OF UNIVERSE
Authors: Handayani Muti , Adha Adrian
Published: January 2025

Penelitian ini bertujuan untuk mengetahui pengaruh Gaya Kepemimpinan Kepala Sekolah SD dan Budaya Kerja terhadap Kepuasan Guru SD pada School of Universe. Penelitian ini menggunakan sampel jenuh sebanyak 30 orang. Teknik pengumpulan data menggunakan angket dengan cara memberikan pernyataan atau pertanyaan tertulis kepada responden untuk dijawab., sedangkan analisis data menggunakan analisis Regresi Linear Sederhana Dan Regresi Linear Berganda. Hasil penelitian ini menunjukan bahwa terdapat pengaruh positif dan signifikan antara Gaya Kepemimpinan Kepala Sekolah SD dan Budaya Kerja terhadap Kepuasan Guru SD hasil perhitungan: 1) Berdasarkan Uji T-test P-value (sig)=0,00 < 0,05 dapat dikatakan pengaruh Gaya Kepemimpinan Kepala Sekolah  terhadap Kepuasan Guru SD  H0 dtolak dan H1 diterima sehingga ada pengaruh antar variable. Uji koefisien Determinasi antara Gaya Kepemimpinan Kepala Sekolah terhadap Kepuasan Guru SD  sebesar 39,3%. 2) Berdasarkan Uji T-test P-value (sig)=0,00 < 0,05 dapat dikatakan pengaruh Budaya Kerja terhadap Kepuasan Guru SD  H0 dtolak dan H1 diterima sehingga ada pengaruh antar variable. Uji koefisien Determinasi antara Budaya Kerja  terhadap Kepuasan Guru SD  sebesar 59,5%. 3) Berdasarkan Uji T-test P-value (sig)=0,00 < 0,05 dapat dikatakan pengaruh Gaya Kepemimpinan Kepala Sekolah dan Budaya Kerja terhadap Kepuasan Guru SD  H0 dtolak dan H1 diterima sehingga ada pengaruh antar variable. Uji koefisien Determinasi antara Gaya Kepemimpinan Kepala Sekolah terhadap Kepuasan Guru SD  sebesar 60,2%. Dengan demikian dapat disismpulkan bahwa: secara bersamaan pengaruh Gaya Kepemimpinan Kepala Sekolah  dan Budaya Kerja mempunyai pengaruh terhadap Kepuasan.
GAMBARAN PENGETAHUAN IBU HAMIL TENTANG MANFAAT PERTOLONGAN PERSALINAN OLEH TENAGA BIDAN DI KELURAHAN HARJOSARI II
Authors: Henni Safrida Sitompul
Published: January 2025

Pelayanan asuhan kebidanan ibu hamil adalah suatu program yangterencana berupa observasi,edukasi dan penanganan medik pada ibu hamil. Saatini angka kematian Ibu (AKI) di Indonesia tergolong masih cukup tinggi yaitumencapai 228 per 100.000 kelahiran.Tujuan Penelitian ini adalah untuk mengetahuitingkat pengetahuan Ibu Hamil Tentang manfaat Pertolongan Persalinan Olehtenaga BidanPenelitian ini bersifat deskriptif dengan menggunakan data primer yangdiperoleh melalui kuisioner dan data sekunder diperoleh dari tenaga kesehatandan LurahHasil penelitian dari 30 responden mayoritas berpengetahuan kurangsebanyak 17 responden (56,6%) berdasarkan umur mayoritas berpengetahuankurangsebanyak 9 responden(30%) dengan umur <20 tahun,berdasarkanpendidikan mayoritas berpengetahuan kurang sebanyak 8 reponden (26,6%)dengan pendidikan Tamat SD,berdasarkan paritas mayoritas berpengetahuankurang sebanyak 9 responden (30%) dengan paritas 1,berdasarkan sumberinformasi berpengetahuan kurang sebanyak 8 responden (26,6 %) dengan sumberinformasi keluarga.Dari hasil penelitian dapat ditarik kesimpulan bahwa pengetahuan ibu hamiltentang manfaat pertolongan persalinan oleh tenaga bidan masih kurang,hal inidisebabkan masih kurang nya informasi dan penyuluhan dari tenagakesehatan.Disarankan kepada Ibu Hamil agar memeriksakan kehamilannyadengan petugas kesehatan.Kata Kunci : Pengetahuan Ibu HamiL Manfaat Pertolongan Persalinan
GAMBARAN KASUS PLASENTA PREVIA DI RSUP H. ADAM MALIK MEDAN PERIODE JANUARI 2020- DESEMBER 2021
Authors: Kiki Khoiriyani  , Stilistika Nehe
Published: January 2025

Di negara yang sedang berkembang, perdarahan yang salah satunyadisebabkan oleh plasenta previa, hampir selalu menjadi malapetaka besar bagipenderita maupun penolongnya karena dapat menyebabkan kesakitan ataukematian baik pada ibu maupun pada janinnya.Penelitian bersifat Deskritif yang bertujuan untuk mengetahui GambaranKasus Plasenta Previa di RSUP H. Adam Malik Medan Periode Januari 2020 –Desember 2021. Dari hasil penelitian ini penulis menemukan bahwa GambaranKasus Plasenta Previa Berdasarkan Distribusi mayoritas umur ibu>35 tahunsebanyak 36 kasus (41,8%) dan minoritas umur ibu <20 tahun sebanyak 21 kasis(24,5%).Berdasarkan paritas ibu mayoritas 3-5 sebanyak 32 kasus (37,2%) danparitas ibu minoritas>5 sebanyak 14 kasus (16,3%). Berdasarkan riwayat obstetriyang lalu mayoritas abortus sebanyak 25 kasus (29,1%) dan minoritas sectioncaesarea sebanyak 11 kasus (12,8) .Keywords: Plasenta Previa
Uso y características de la Realidad Aumentada (RA) en los informativos de televisión españoles: los casos de Antena 3 y TVE1
Authors: García-Aguilera Amparo , González-Cortés María Eugenia , Gómez-Calderón Bernardo
Published: January 2025

Este artículo examina el uso que hacen de la Realidad Aumentada (RA) TVE y Antena 3, a partir de un análisis comparativo, y observa la incidencia que tiene esta tecnología en la rutina profesional de los periodistas. El trabajo se apoya en un análisis de contenido de las piezas de RA aparecidas en los telediarios de la franja nocturna durante los meses de enero y febrero de 2023 (N= 343) y en la realización de cuatro entrevistas personales a los jefes de realización y presentadores de dichos espacios. Los resultados apuntan a que la RA ha crecido exponencialmente en Antena 3, al tiempo que mengua en el caso del ente público; su uso es, por el momento, meramente complementario y guiado sobre todo por fines estéticos, empleado bien para adornar la información que suministra el conductor de informativos, bien para ampliar o caracterizar el plató. La transformación en las rutinas profesionales es evidente, según los propios informadores: la forma de contar las noticias e incluso el tono han cambiado, obligando a un esfuerzo extra para mantener el rigor informativo sin caer en el sensacionalismo.
Lo queer en lo transmedia: Análisis del videojuego y la serie The Last of Us
Authors: Higueras-Ruiz María-José , Pérez-Rufí José-Patricio
Published: January 2025

Las opciones de la digitalización y la convergencia en un contexto transmedial permiten expandir los universos narrativos de la ficción a través de diferentes medios. Este es el caso del videojuego y la serie de ficción, cuyo estudio comparativo es el foco de atención académica en esta investigación, concretamente, desde la representación social del colectivo LGTBIQ+. El objetivo principal de este trabajo es determinar la presencia y representación queer en el universo de The Last of Us, seleccionado por su popularidad y aceptación, y considerando dicha ficción como un caso paradigmático desde este sentido. Con un alcance cualitativo se aplica el análisis textual para comparar la representación de dos parejas de personajes homosexuales en ambos formatos: Bill y Frank, y Ellie y Riley. Los resultados aplauden la inclusión queer en el universo ficcional, especialmente en la serie de televisión, anotando ciertas carencias y estereotipos en el videojuego original en lo relativo a la trama masculina. A pesar de ello, la inclusión es positiva y la aceptación patente, lo que repercute favorablemente en la identificación de la comunidad LGTBIQ+ en el audiovisual y el reconocimiento de la sociedad en general, atendiendo al papel de los medios como instrumentos de socialización.
Mediator kinase inhibition suppresses hyperactive interferon signaling in Down syndrome
Authors: Cozzolino Kira , Sanford Lynn , Hunter Samuel , Molison Kayla , Erickson Benjamin , Courvan Meaghan CS , Jones Taylor , Ajit Deepa , Galbraith Matthew D , Espinosa Joaquin M , Bentley David L , Allen Mary A , Dowell Robin D , Taatjes Dylan J
Published: January 2025

Hyperactive interferon (IFN) signaling is a hallmark of Down syndrome (DS), a condition caused by trisomy 21 (T21); strategies that normalize IFN signaling could benefit this population. Mediator-associated kinases CDK8 and CDK19 drive inflammatory responses through incompletely understood mechanisms. Using sibling-matched cell lines with/without T21, we investigated Mediator kinase function in the context of hyperactive IFN in DS over a 75min - 24h timeframe. Activation of IFN-response genes was suppressed in cells treated with the CDK8/CDK19 inhibitor cortistatin A (CA), via rapid suppression of IFN-responsive transcription factor (TF) activity. We also discovered that CDK8/CDK19 affect splicing, a novel means by which Mediator kinases control gene expression. To further probe Mediator kinase function, we completed cytokine screens and metabolomics experiments. Cytokines are master regulators of inflammatory responses; by screening 105 different cytokine proteins, we show that Mediator kinases help drive IFN-dependent cytokine responses at least in part through transcriptional regulation of cytokine genes and receptors. Metabolomics revealed that Mediator kinase inhibition altered core metabolic pathways in cell type-specific ways, and broad up-regulation of anti-inflammatory lipid mediators occurred specifically in kinase-inhibited cells during hyperactive IFNγ signaling. A subset of these lipids (e.g. oleamide, desmosterol) serve as ligands for nuclear receptors PPAR and LXR, and activation of these receptors occurred specifically during hyperactive IFN signaling in CA-treated cells, revealing mechanistic links between Mediator kinases, lipid metabolism, and nuclear receptor function. Collectively, our results establish CDK8/CDK19 as context-specific metabolic regulators, and reveal that these kinases control gene expression not only via TFs, but also through metabolic changes and splicing. Moreover, we establish that Mediator kinase inhibition antagonizes IFN signaling through transcriptional, metabolic, and cytokine responses, with implications for DS and other chronic inflammatory conditions.
Human eIF2A has a minimal role in translation initiation and in uORF-mediated translational control
Authors: Roiuk Mykola , Neff Marilena , Teleman Aurelio A
Published: January 2025

Initiation of translation on eukaryotic mRNAs requires a 40S ribosome loaded with an initiator tRNA in order to scan for, and to identify, an initiation codon. Under most conditions, the initiator tRNA is recruited to the ribosome as part of a ternary complex composed of initiator tRNA, eIF2 and GTP. Although this function of recruiting the initiator tRNA was originally ascribed to another factor, eIF2A, it was later disproven and shown to belong to eIF2. Nonetheless, eIF2A is still considered a translation initiation factor because it binds the ribosome and shows genetic interactions with other initiation factors such as eIF4E. The exact function of eIF2A during translation initiation, however, remains unclear. We systematically test here by ribosome profiling and luciferase reporter assays the role of eIF2A in translation initiation, including translation of upstream ORFs that are either initiated with a canonical AUG or near-cognate codons. Since eIF2A is thought to take over the function of eIF2 when eIF2 is inhibited, we also test conditions where the integrate stress response is activated, thereby leading to eIF2 inactivation. In none of our assays, however, could we detect a role of eIF2A in translation initiation. We propose that instead eIF2A may be playing a function related to other aspects of RNA biology.
Unbiased identification of cell identity in dense mixed neural cultures
Authors: De Beuckeleer Sarah , Van De Looverbosch Tim , Van Den Daele Johanna , Ponsaerts Peter , De Vos Winnok H
Published: January 2025

Induced pluripotent stem cell (iPSC) technology is revolutionizing cell biology. However, the variability between individual iPSC lines and the lack of efficient technology to comprehensively characterize iPSC-derived cell types hinder its adoption in routine preclinical screening settings. To facilitate the validation of iPSC-derived cell culture composition, we have implemented an imaging assay based on cell painting and convolutional neural networks to recognize cell types in dense and mixed cultures with high fidelity. We have benchmarked our approach using pure and mixed cultures of neuroblastoma and astrocytoma cell lines and attained a classification accuracy above 96%. Through iterative data erosion, we found that inputs containing the nuclear region of interest and its close environment, allow achieving equally high classification accuracy as inputs containing the whole cell for semi-confluent cultures and preserved prediction accuracy even in very dense cultures. We then applied this regionally restricted cell profiling approach to evaluate the differentiation status of iPSC-derived neural cultures, by determining the ratio of postmitotic neurons and neural progenitors. We found that the cell-based prediction significantly outperformed an approach in which the population-level time in culture was used as a classification criterion (96% vs 86%, respectively). In mixed iPSC-derived neuronal cultures, microglia could be unequivocally discriminated from neurons, regardless of their reactivity state, and a tiered strategy allowed for further distinguishing activated from non-activated cell states, albeit with lower accuracy. Thus, morphological single-cell profiling provides a means to quantify cell composition in complex mixed neural cultures and holds promise for use in the quality control of iPSC-derived cell culture models.
Knockout of cyclin dependent kinases 8 and 19 leads to depletion of cyclin C and suppresses spermatogenesis and male fertility in mice
Authors: Bruter Alexandra V , Varlamova Ekaterina A , Stavskaya Nina I , Antysheva Zoia G , Manskikh Vasily N , Tvorogova Anna V , Korshunova Diana. S , Khamidullina Alvina I , Utkina Marina V , Bogdanov Viktor P , Baikova Iuliia P , Nikiforova Alyona I , Albert Eugene A , Maksimov Denis O , Li Jing , Chen Mengqian , Schools Gary. P , Feoktistov Alexey V , Shtil Alexander A , Roninson Igor B , Mogila Vladislav A , Silaeva Yulia Y , Tatarskiy Victor V
Published: January 2025

Paralogs CDK8 and CDK19 are regulatory kinases associated with the transcriptional Mediator complex. We have e generated mice with the systemic inducible
Cdk8
knockout on the background of
Cdk19
constitutive knockout.
Cdk8/19
double knockout (iDKO) males, but not single
Cdk8
and
Cdk19
KO, had an atrophic reproductive system and were infertile. The iDKO males lacked postmeiotic spermatids and spermatocytes after meiosis I pachytene. Testosterone levels were decreased whereas the amounts of the luteinizing hormone were unchanged. Single cell RNA sequencing showed marked differences in the expression of steroidogenic genes (such as
Cyp17a1, Star
and
Fads
) in Leydig cells concomitant with alterations in Sertoli cells and spermatocytes likely associated with impaired synthesis of steroids.
Star
and
Fads
were also downregulated in cultivated Leydig cells after iDKO. The treatment of primary Leydig cells culture with a CDK8/19 inhibitor did not induce the same changes in gene expression as iDKO, and prolonged treatment of mice with a CDK8/19 inhibitor did not affect the size of testes. iDKO, in contrast to single knockouts or treatment with a CDK8/19 kinase inhibitor, led to depletion of cyclin C (CcnC), the binding partner of CDK8/19 that has been implicated in CDK8/19-independent functions. This suggests that the observed phenotype was likely mediated through kinase-independent activities of CDK8/19, such as CcnC stabilization.
MSSA: multi-stage semantic-aware neural network for binary code similarity detection
Authors: Wan Bangrui , Zhou Jianjun , Wang Ying , Chen Feng , Qian Ying
Published: January 2025

Binary code similarity detection (BCSD) aims to identify whether a pair of binary code snippets is similar, which is widely used for tasks such as malware analysis, patch analysis, and clone detection. Current state-of-the-art approaches are based on Transformer, which require substantial computation resources. Learning-based approaches remains room for optimization in learning the deeper semantics of binary code. In this paper, we propose MSSA, a multi-stage semantic-aware neural network for BCSD at the function level. It effectively integrates the semantic and structural information of assembly instructions within and between basic blocks, and across the entire function through four semantic-aware neural networks, achieving deep understanding of binary code semantics. MSSA is a lightweight model with only 0.38M parameters in its backbone network, suitable for deployment in CPU environments. Experimental results show that MSSA outperforms Gemini, Asm2Vec, SAFE, and jTrans in classification performance and ranks second only to the Transformer-based jTrans in retrieval performance.
Enhancing Agricultural Water Efficiency through Conservation and Groundwater Management in Akola District, Vidarbha Region
Authors: Patode R. S. , Chorey Anita , Wankhade Ranee , Ganvir M. M. , Pandagale V. P. , Mali R. S. , Morey Sachin , Fukat Pawan , Sinha M.
Published: January 2025

Rainfall is the single input in the Dryland agriculture for fulfilling the crop water requirement. Based on the watershed approach it is possible to make a quantitative evaluation of water resources and their change under the influence of man's activities. Knowledge of the water availability assists the prediction of the consequences of artificial changes in the regime of streams, lakes, and ground-water basins. An understanding of the water balance is also extremely important for providing protective irrigations. To explore the impact of water conservation configurations on groundwater establishment at Kajaleshwar micro watershed of Tq. Barshitakli, Distt. Akola in Vidarbha region of Maharashtra the study was completed under Agri- Consortia Research Platform on Water, All India Coordinated Research Project for Dryland Agriculture, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. Drainage (Nala), widening and deepening and repairs of established rainwater harvesting and storage structures were ensured for impact evaluation. Groundwater data was collected for 51 observation wells in the vicinity of 10 cement nala bunds (CNBs) for analysis of rainwater harvesting influence on groundwater regime in micro watershed area. During 2017 (36 wells) average depth of water level was 4.07 m and during 2019 (36+15= 51wells) it was increased and found to be 2.81m. Well water levels were observed to be increased in 2019 as compared to 2017. The yield of soybean crop with one protective irrigation at pod filling stage during Kharif season was (1483 kg/ha) increased by 26 % as compared to without irrigation (1180 kg/ha). During Rabi season on an average the yield of Chickpea crop with two protective irrigations at initial and pod filling stage was (1773 kg/ha) increased by 38.73% as compared to without irrigation (1278 kg/ha). In the event of a protective irrigation, the yield of Chickpea was (1539kg/ha) increased by 20.42 % as compared to without irrigation (1278 kg/ha). Thus, it can be inferred that if conservation measures are implemented technically then yield sustainability in agriculture can be achieved. Due to acceptance of comparable types of rainwater harvesting and non-natural recharge structures, groundwater possessions will be augmented. This will support in expansion of integrated farming systems which eventually will be helpful in increasing the yields and water efficiency of the area where such works are being accepted.
Occupational Risks of Microplastics in Textile Manufacturing: Health Impacts and Mitigation Strategies
Authors: Medeiros Julia Clara Gomes de , Luz Débora Kauanna Régis da , Sousa Anésio Mendes de , Júnior Geraldo Barroso Cavalcanti , Kramer Dany Geraldo
Published: January 2025

Background: In the textile sector, the atmospheric release of MPs during the manufacturing, drying and use of synthetic fabrics has also been observed, in addition to the sewing process, where small structural changes in the fabric network favor the release of polyester and polypropylene fibrils In this context, the present study aimed to discuss, through a literature review, occupational exposure to MPs in the textile manufacturing sector.
Methodology: This study was characterized as a descriptive bibliographic review, focusing on the search for theoretical references in scientific journals, theses and dissertations.
Results and Conclusions: To reduce employee exposure, it is important to properly use personal protective equipment, including face masks, which reduce exposure to microfibers in the oral and nasal mucosa, reducing the possibility of ingestion and inhalation of these. Furthermore, an adequate ventilation and exhaust system makes it possible to remove suspended particles from the work atmosphere, and must be combined with filters to prevent release into the factory's external environment.ential, as they mitigate the exposure of the upper respiratory tract and mouth to microplastics released into the environment.
Biodiversity of Aquatic Insects in Kalsa River (Chanfi) of Nainital District, Uttarakhand, India
Authors: Singh Vishal , Noor Arooba , Goswami Deepika
Published: January 2025

Aquatic insects are the most species-rich group that inhabit freshwaters. They are connected to water by at least one life stage, usually that of the larvae, and some spend their entire life in freshwater habitats. The majority of aquatic insects’ larvae develop in water; while adults emerge and spend their lives primarily in terrestrial environments where they mate, disperse and in some cases feed. Aquatic insects are important components of stream food webs and are greatly impacted by anthropogenic disturbances, including urbanization. A study was conducted in Kalsa River, Nainital District to understand the diversity of insects in the area by using sweep-net method and analysis by respected equation and indexes. The study recorded 259 numbers of individuals which divided into 4 orders, namely Lepidoptera, Hymenoptera, Coleoptera, Diptera, and Orthoptera. Trichoptera, Odonata and Hemiptera was dominant order, were as Diptera is least dominant among all the reported orders. The Shannon-Wiener diversity index was highest in site, at (H’=1.79). Evenness was highest at (E=0.38), and the Margalef’s Richness Index was (d=1.50). Overall, this study offers a novel method for predicting aquatic insect distribution with high geographic resolution, which aids in the protection of river diversity in the face of anthropogenic and climatic change also this study aimed to identify aquatic insects’ species in and provide baseline information for future research.
Resources Use Efficiency and Resources Utilization Efficiency of Major Crops in North Gujarat, India
Authors: Patel Umang B , Leua Alpesh , Patel Parul M , Joshi Rahul , Padaliya Meera
Published: January 2025

A study was examined to resource use efficiency and resources utilization efficiency of major crops in North Gujarat. Utilizing both canal and tube-well irrigation systems. Cobb-Douglas production function and MVP/MFC ratio was used for calculation of resources use efficiency and resources utilization efficiency, respectively. Primary data were collected from 160 respondents with the pretested interview schedule during 2023-24. The research revealed that, nitrogen, phosphorus and machinery as pivotal resources influencing yield outcomes. In wheat production, nitrogen and phosphorus had significant positive effects on yield in canal-irrigated fields, while over-irrigation negatively impacted tube-well-irrigated yields. Cotton analysis highlighted nitrogen’s positive yield impact under tube-well irrigation in Mehsana, while excessive machinery uses reduced productivity across both irrigation types. For castor, nitrogen and phosphorus boosted yield in canal-irrigated systems, although machinery use adversely affected output, particularly in Patan. Labor was found to positively influence castor yield under tube-well irrigation, underscoring the need for balanced mechanization. Higher R² values in Mehsana and Patan showed a robust model fit. Overall, the resources utilization efficiency revealed that, major input such as nitrogen, phosphorus and machinery was underutilization in selected crops and irrigation and labour was found as over-utilization. Therefore, the research suggests that increasing the use of underutilized inputs and reducing the use of overutilized resources could improve production, farm management and cost-effectiveness
Development of Bioprocess Technology for Parasite Control in Aquaculture Commodities
Authors: Aisyah  , Pratama Gilar Budi
Published: January 2025

Controlling parasites in cultivated commodities is a crucial challenge in the fisheries industry that can impact fish health and production quality. The use of conventional methods such as chemical drugs often results in negative side effects on the environment and human health. Therefore, the development of bioprocess technology offers a more environmentally friendly and sustainable solution for parasite management. In bioprocess technology, probiotic microorganisms are used to enhance fish immune responses, enzymes such as proteases or lipases are used to lower the number of parasites. Additionally, research also focuses on the development of vaccines to target specific parasites, providing a more permanent control alternative. By reducing reliance on synthetic chemicals and minimizing parasite resistance, these technologies enhance the sustainability of aquaculture. The development and application of this bioprocess technology have the potential to enhance the efficiency and sustainability of aquaculture production. Future research could explore the integration of advanced genetic engineering and microbiome manipulation to enhance the precision and efficacy of bioprocess technologies for parasite control in aquaculture.
In-vitro Nematicidal Activity of Different Solvent Extracts of Solanum torvum Fruit against Meloidogyne incognita
Authors: V Basavaraj , Aralikatti Sunita , Sharada M S , M R Sampathkumar , H M Mahesh , Sonappanavar Arunkumar B
Published: January 2025

Root-knot nematodes are harmful plant parasites that significantly reduce agricultural productivity, affecting about 2,000 plant species and causing 5% of global crop losses. Due to concerns about the environmental impact of chemical nematicides, plant-based alternatives are gaining attention. This study, conducted in September-October 2023 at the University of Mysore, tested four solvent extracts (aqueous, petroleum ether, ethanol, and methanol) from Solanum torvum fruit at varying concentrations (10-100%) for their effects on egg hatchability and juvenile mortality of Meloidogyne incognita. Results showed that the methanolic extract was most effective, achieving 99% inhibition of egg hatching and 100% juvenile mortality at 100% concentration. This suggests that Solanum torvum extract could be an eco-friendly and economical method for managing root-knot nematodes. Further research is needed to evaluate its field efficacy and to identify the active compounds responsible for its nematicidal properties.
Empowering Small-Scale Vegetable Farmers with Drone-based Decision Support Systems for Sustainable Production
Authors: Chauhan Mayank , Negi Radhika , Ravi  , Dutta Anirban , Sahoo Sweta , Panotra Narinder , K.P.Sivakumar  , K Sruthi , Kumar Hirdesh , Singh Bal veer
Published: January 2025

In India, small-scale vegetable farmers face significant challenges in achieving sustainable and profitable production due to limited access to modern technologies and decision support tools. This study explores the potential of drone-based remote sensing and decision support systems (DSS) to empower small-scale farmers and promote sustainable vegetable production practices. The research involved deploying multispectral sensor-equipped drones over 50 smallholder vegetable farms in the state of Maharashtra, India to collect high-resolution crop health and growth data across multiple growing seasons. The aerial data was processed and integrated into a cloud-based DSS platform that provided participating farmers with actionable insights and recommendations to optimize irrigation, fertilization, pest/disease control, and harvest scheduling. The DSS also incorporated weather forecasts, market price information, and expert agronomic knowledge to help farmers make informed decisions. However, challenges remain in building digital literacy, trust, and infrastructure to enable wider adoption among smallholder farmers. Future work should focus on participatory design of DSSs, integration with existing agricultural extension services, and inclusive business models for delivering precision agriculture technologies to small-scale farmers in developing countries.
Study on Growth, Yield and Quality of Pea under the Influence of Different Combinations of Biofertilizers and Organic Manures
Authors: Rawat Himani , Singh K.C. , Pant S.C.
Published: January 2025

The Pea (Pisum sativum) belongs to group of the legume family (Fabaceae).  The immature green seeds are eaten raw, cooked, dehydrated, or as the main ingredient in frozen vegetable dishes. The present investigation was carried out at Vegetable Research & Demonstration Block, College of Horticulture, VCSG Uttarakhand University of Horticulture and Forestry, Bharsar Pauri Garhwal during Rabi season (2023-2024) to access the performance of pea to different organic cultivation practices. The experiment was laid out in Randomized Complete block Design (RCBD) with 13 treatments and 3 replications. The treatments were a combination of different doses of biofertilizers i.e. (Rhizobium leguminosarum) and PSB along with application of organic manures (FYM, Vermicompost and Compost). Parameters like germination (93.333 %), plant height (67.813 cm), leaf count (60.333), RLWC % (80.963 %), number of cluster/plant (13.633), number of root nodules (27.433), root active nodules (14.217), pod length (10.267 cm), pod yield/plant (77.230), seed yield/plant (60.760 g), shelling percentage (48.920 %) and 100 seed weight (46.470 g), TSS (14.0 °Brix) and Ascorbic acid (25 mg/100g) also found highest in T9. The data depicted best results in T9 for all the treatments which was found statistically at par with T5.  But considering economic point of view Cost benefit ratio (1:1.73) was found to be maximum under treatment T5 [Rhizobium leguminosarum (20ml) + PSB (20ml) +FYM (10 t/ha)] and should be recommended to the farmers as for deriving maximum profit for organic cultivation of pea. Therefore, the current study concludes that organic sources of nutrients are more environment friendly and sustainable than chemical fertilizers. They also have an advantage over inorganic synthetic fertilizers, which pollute the environment and may accumulate in the soil and lead to health risk of living surrounding beings.