bluuebunny/crossref_metadata_2025 huggingface dataset:
Predicting the Structure of Enzymes with Metal Cofactors: The Example of [FeFe] Hydrogenases
Authors: Botticelli Simone , La Penna Giovanni , Minicozzi Velia , Stellato Francesco , Morante Silvia , Rossi Giancarlo , Faraloni Cecilia
Published: March 2024

The advent of deep learning algorithms for protein folding opened a new era in the ability of predicting and optimizing the function of proteins once the sequence is known. The task is more intricate when cofactors like metal ions or small ligands are essential to functioning. In this case, the combined use of traditional simulation methods based on interatomic force fields and deep learning predictions is mandatory. We use the example of [FeFe] hydrogenases, enzymes of unicellular algae promising for biotechnology applications to illustrate this situation. [FeFe] hydrogenase is an iron–sulfur protein that catalyzes the chemical reduction of protons dissolved in liquid water into molecular hydrogen as a gas. Hydrogen production efficiency and cell sensitivity to dioxygen are important parameters to optimize the industrial applications of biological hydrogen production. Both parameters are related to the organization of iron–sulfur clusters within protein domains. In this work, we propose possible three-dimensional structures of Chlorella vulgaris 211/11P [FeFe] hydrogenase, the sequence of which was extracted from the recently published genome of the given strain. Initial structural models are built using: (i) the deep learning algorithm AlphaFold; (ii) the homology modeling server SwissModel; (iii) a manual construction based on the best known bacterial crystal structure. Missing iron–sulfur clusters are included and microsecond-long molecular dynamics of initial structures embedded into the water solution environment were performed. Multiple-walkers metadynamics was also used to enhance the sampling of structures encompassing both functional and non-functional organizations of iron–sulfur clusters. The resulting structural model provided by deep learning is consistent with functional [FeFe] hydrogenase characterized by peculiar interactions between cofactors and the protein matrix.
Gene Expression Patterns Associated with Survival in Glioblastoma
Authors: Morrison Christopher , Weterings Eric , Gravbrot Nicholas , Hammer Michael , Weinand Martin , Sanan Abhay , Pandey Ritu , Mahadevan Daruka , Stea Baldassarre
Published: March 2024

The aim of this study was to investigate gene expression alterations associated with overall survival (OS) in glioblastoma (GBM). Using the Nanostring nCounter platform, we identified four genes (COL1A2, IGFBP3, NGFR, and WIF1) that achieved statistical significance when comparing GBM with non-neoplastic brain tissue. The four genes were included in a multivariate Cox Proportional Hazard model, along with age, extent of resection, and O6-methylguanine-DNA methyltransferase (MGMT) promotor methylation, to create a unique glioblastoma prognostic index (GPI). The GPI score inversely correlated with survival: patient with a high GPI had a median OS of 7.5 months (18-month OS = 9.7%) whereas patients with a low GPI had a median OS of 20.1 months (18-month OS = 54.5%; log rank p-value = 0.004). The GPI score was then validated in 188 GBM patients from The Cancer Genome Atlas (TCGA) from a national data base; similarly, patients with a high GPI had a median OS of 10.5 months (18-month OS = 12.4%) versus 16.9 months (18-month OS = 41.5%) for low GPI (log rank p-value = 0.0003). We conclude that this novel mRNA-based prognostic index could be useful in classifying GBM patients into risk groups and refine prognosis estimates to better inform treatment decisions or stratification into clinical trials.
Effect of Probiotics in Stress-Associated Constipation Model in Zebrafish (Danio rerio) Larvae
Authors: Lee Ayoung , Kim Seung Young , Kang Seyoung , Kang Seong Hee , Kim Dong Woo , Choe Jung Wan , Hyun Jong Jin , Jung Sung Woo , Jung Young Kul , Koo Ja Seol , Yim Hyung Joon , Kim Suhyun
Published: March 2024

The pathophysiology of functional bowel disorders is complex, involving disruptions in gut motility, visceral hypersensitivity, gut–brain–microbiota interactions, and psychosocial factors. Light pollution, as an environmental stressor, has been associated with disruptions in circadian rhythms and the aggravation of stress-related conditions. In this study, we investigated the effects of environmental stress, particularly continuous light exposure, on intestinal motility and inflammation using zebrafish larvae as a model system. We also evaluated the efficacy of probiotics, specifically Bifidobacterium longum (B. longum), at alleviating stress-induced constipation. Our results showed that continuous light exposure in zebrafish larvae increased the cortisol levels and reduced the intestinal motility, establishing a stress-induced-constipation model. We observed increased inflammatory markers and decreased intestinal neural activity in response to stress. Furthermore, the expressions of aquaporins and vasoactive intestinal peptide, crucial for regulating water transport and intestinal motility, were altered in the light-induced constipation model. Administration of probiotics, specifically B. longum, ameliorated the stress-induced constipation by reducing the cortisol levels, modulating the intestinal inflammation, and restoring the intestinal motility and neural activity. These findings highlight the potential of probiotics to modulate the gut–brain axis and alleviate stress-induced constipation. Therefore, this study provides a valuable understanding of the complex interplay among environmental stressors, gut function, and potential therapeutic strategies.
Advancing Newborn Screening Long-Term Follow-Up: Integration of Epic-Based Registries, Dashboards, and Efficient Workflows
Authors: Raboin Katherine , Ellis Debra , Nichols Ginger , Hughes Marcia , Brimacombe Michael , Rubin Karen
Published: March 2024

The Connecticut Newborn Screening (NBS) Network, in partnership with the Connecticut Department of Public Health, strategically utilized the Epic electronic health record (EHR) system to establish registries for tracking long-term follow-up (LTFU) of NBS patients. After launching the LTFU registry in 2019, the Network obtained funding from the Health Resources and Services Administration to address the slow adoption by specialty care teams. An LTFU model was implemented in the three highest-volume specialty care teams at Connecticut Children’s, involving an early childhood cohort diagnosed with an NBS-identified disorder since the formation of the Network in March 2019. This cohort grew from 87 to 115 over the two-year project. Methods included optimizing registries, capturing external data from Health Information Exchanges, incorporating evidence-based guidelines, and conducting qualitative and quantitative evaluations. The early childhood cohort demonstrated significant and sustainable improvements in the percentage of visits up-to-date (%UTD) compared to the non-intervention legacy cohort of patients diagnosed with an NBS disorder before the formation of the Network. Positive trends in the early childhood cohort, including %UTD for visits and condition-specific performance metrics, were observed. The qualitative evaluation highlighted the achievability of practice behavior changes for specialty care teams through responsive support from the nurse analyst. The Network’s model serves as a use case for applying and achieving the adoption of population health tools within an EHR system to track care delivery and quickly fill identified care gaps, with the aim of improving long-term health for NBS patients.
Spatial Computational Hepatic Molecular Biomarker Reveals LSEC Role in Midlobular Liver Zonation Fibrosis in DILI and NASH Liver Injury
Authors: Puri Munish
Published: March 2024

The liver is structurally organized into zonation, where Liver Sinusoidal Endothelial Cells (LSECs) play a crucial role during chronic liver injury and the early stages of fibrosis. Fibrosis can be reversed if diagnosed early at the molecular level in zonation before progressing to advanced stages like bridging fibrosis. This study identified zonation marker genes using scRNA-seq and spatial transcriptomics molecular profiling technologies in a normal and diseased fibrotic human liver. DGE analysis was performed over LSECs, and we identified the top 20 expressed genes in the periportal, perivenous, and intermediate acinar zones. Multi-omics and scRNA-seq analysis over Visium images and ECs liver cells showed OIT3, DNASE1L3, CLEC4G, LYVE1, FCN2, and CRHBP as commonly expressed mid-lobular zonation-specific genes. Also, this study detected STAB2, F8, AQP1, TEK, TIMP3, TIE1, and CTSL genes as expressed in DILI and NASH EC populations. The connection between LSEC marker genes in zone 2 and liver fibrosis holds significant promise for advancing our understanding in developing new therapeutic strategies for fibrosis reversal and designing computational molecular biomarkers in NASH and DILI fibrotic liver diseases.
Towards Sustainable Road Pavements: Sound Absorption in Rubber-Modified Asphalt Mixtures
Authors: Apaza Freddy Richard , Fernández Vázquez Víctoriano , Paje Santiago Expósito , Gulisano Federico , Gagliardi Valerio , Saiz Rodríguez Leticia , Gallego Medina Juan
Published: March 2024

In the last decade, various asphalt paving materials have undergone investigation for sound attenuation purposes. This research aims to delve into the innovative design of sustainable road pavements by examining sound absorption in rubber-modified asphalt mixtures. More specifically, the impact of alternative sustainable materials on the sound absorption of asphalt mixtures across different temperatures, precisely crumb rubber (CR) derived from recycling of end-of-life tires, was investigated. The acoustic coefficient and its Gaussian fit parameters (Peak, BandWidth, and Area Under the Curve) were evaluated. Five different types of asphalt mixtures were studied, encompassing dense, discontinuous, and open mixtures with 0%, 0.75%, and 1.50% CR incorporated through the dry process (DP). The results of sound absorption indicated a slight influence of crumb rubber at temperatures ranging from 10 °C to 60 °C, particularly in mixtures with high void content. On the other hand, as expected, the void content proved to be highly correlated with sound absorption. These findings facilitated the establishment of predictive models that correlate acoustic absorption spectra with the characteristics of asphalt mixtures. As a result, these models will be valuable in the design of the next generation of sound-absorbing pavements.
The Relationship between Subclinical Hypothyroidism and Carotid Intima-Media Thickness as a Potential Marker of Cardiovascular Risk: A Systematic Review and a Meta-Analysis
Authors: Isailă Oana-Maria , Stoian Victor Eduard , Fulga Iuliu , Piraianu Alin-Ionut , Hostiuc Sorin
Published: March 2024

Background and Objectives: Thyroid dysfunction is known to have significant consequences on the cardiovascular system. The correlation between carotid intima-media thickness (CIMT) and subclinical hypothyroidism (SCH) has been frequently evaluated in clinical studies in recent years. This study aimed to evaluate the significance of this association through a meta-analysis. Methods: We conducted a systematic search of PubMed, MedLine, Scopus, and Web of Science databases using the keywords ‘subclinical hypothyroidism and carotid intima-media thickness’, from the beginning of each database until January 2023. We established the inclusion and exclusion criteria and considered studies that met the inclusion criteria. We used Jamovi for statistical analysis of the data. Results: We identified 39 observational studies that met the inclusion criteria, with 3430 subjects: 1545 SCH and 1885 EU. Compared to euthyroid subjects (EU), subjects with subclinical hypothyroidism (SCH) had significantly increased carotid intima-media thickness (CIMT) values; the estimated average mean difference was 0.08 (95% CI 0.05 to 0.10), p < 0.01, I2 = 93.82%. After the sensitivity analysis, a total of 19 from the 39 abovementioned studies were analyzed, with most studies showing a positive association between SCH and thickening of the carotid wall; the estimated average mean difference was 0.04 (95% CI 0.02 to 0.07), p = 0.03, I2 = 77.7. In addition, female sex, advanced age, and high cholesterol levels statistically significantly influenced this association. Conclusions: Our meta-analysis indicates a significant positive association between SCH and increased CIMT, but with some limitations.
Clinical Efficacy of 10 Min of Active Prewarming for Preserving Patient Body Temperature during Percutaneous Nephrolithotomy: A Prospective Randomized Controlled Trial
Authors: Shim Jung-Woo , Kwon Hyejin , Moon Hyong Woo , Chae Min Suk
Published: March 2024

Background: Percutaneous nephrolithotomy (PNL) poses a risk of hypothermia. Additionally, general anesthesia lowers the thresholds for shivering and vasoconstriction, which leads to dysfunction of central thermoregulation. Perioperative hypothermia is associated with adverse outcomes after surgery. In this study, we aimed to demonstrate that prewarming for 10 min can effectively prevent early hypothermia during PNL. Methods: A total of 68 patients scheduled for elective PNL were recruited to this study from January to June 2022, but two patients were excluded because of a change in the surgical plan. After randomization, patients in the prewarming group (n = 32) received warming using a forced-air warming device for 10 min in the preoperative area before being transferred to the operating room, while the controls (n = 34) did not. The incidence of hypothermia within the first hour after inducing general anesthesia was the primary outcome. Perioperative body temperatures and postoperative recovery findings were also evaluated. Results: Early intraoperative hypothermia decreased significantly more in the prewarming group than in the control group (9.4% vs. 41.2%, p = 0.003). Moreover, the net decrease in core body temperature during surgery was smaller in the prewarming group than in the control group (0.2 °C, vs. 0.5 °C, p = 0.003). In addition, the prewarmed patients had a lower incidence of postoperative shivering and a shorter post-anesthesia-care unit (PACU) stay (12.5% vs. 35.3%, p = 0.031; and 46 vs. 50 min, p = 0.038, respectively). Conclusions: Prewarming for 10 min decreased early hypothermia, preserved intraoperative body temperature, and improved postoperative recovery in the PACU.
Oscillometry Longitudinal Data on COVID-19 Acute Respiratory Distress Syndrome Treated with Non-Invasive Respiratory Support
Authors: Torregiani Chiara , Baratella Elisa , Segalotti Antonio , Ruaro Barbara , Salton Francesco , Confalonieri Paola , Tavano Stefano , Lapadula Giulia , Bozzi Chiara , Confalonieri Marco , Dellaca’ Raffaele , Veneroni Chiara
Published: March 2024

Background: Oscillometry allows for the non-invasive measurements of lung mechanics. In COVID-19 ARDS patients treated with Non-Invasive Oxygen Support (NI-OS), we aimed to (1) observe lung mechanics at the patients’ admission and their subsequent changes, (2) compare lung mechanics with clinical and imaging data, and (3) evaluate whether lung mechanics helps to predict clinical outcomes. Methods: We retrospectively analyzed the data from 37 consecutive patients with moderate–severe COVID-19 ARDS. Oscillometry was performed on their 1st, 4th, and 7th day of hospitalization. Resistance (R5), reactance (X5), within-breath reactance changes (ΔX5), and the frequency dependence of the resistance (R5–R19) were considered. Twenty-seven patients underwent computed tomographic pulmonary angiography (CTPA): collapsed, poorly aerated, and normally inflated areas were quantified. Adverse outcomes were defined as intubation or death. Results: Thirty-two patients were included in this study. At the first measurement, only 44% of them had an abnormal R5 or X5. In total, 23 patients had measurements performed on their 3rd day and 7 on their 7th day of hospitalization. In general, their R5, R5–R19, and ΔX decreased with time, while their X5 increased. Collapsed areas on the CTPA correlated with the X5 z-score (ρ = −0.38; p = 0.046), while poorly aerated areas did not. Seven patients had adverse outcomes but did not present different oscillometry parameters on their 1st day of hospitalization. Conclusions: Our study confirms the feasibility of oscillometry in critically ill patients with COVID-19 pneumonia undergoing NI-OS. The X5 z-scores indicates collapsed but not poorly aerated lung areas in COVID-19 pneumonia. Our data, which show a severe impairment of gas exchange despite normal reactance in most patients with COVID-19 ARDS, support the hypothesis of a composite COVID-19 ARDS physiopathology.
Idiopathic Premature Ventricular Contraction Catheter Ablation, Sedentary Population vs. Athlete’s Populations: Outcomes and Resumption of Sports Activity
Authors: Valeri Yari , Compagnucci Paolo , Volpato Giovanni , Luciani Lara , Crepaldi Eleonora , Maiorino Francesco , Parisi Quintino , Cipolletta Laura , Campanelli Francesca , D’Angelo Leonardo , Gaggiotti Gemma , Gasperetti Alessio , Giovagnoni Andrea , Curcio Antonio , Dello Russo Antonio , Casella Michela
Published: March 2024

There are no investigations about the outcomes of idiopathic PVC catheter ablation (CA) in athletes compared to the sedentary population. We conducted a prospective single-centre observational study. The primary and secondary procedural outcomes were the post-ablation reduction of premature ventricular contractions (PVCs) in an athletes vs. non-athletes group and in agonist vs. leisure-time athletes. The third was the evaluation of the resumption of physical activity and the improvement of symptoms in agonist and leisure-time athletes. From January 2020 to October 2022 we enrolled 79 patients with RVOT/LVOT/fascicular PVC presumed origin. The median percentage of decrease between the pre-procedure and post-procedure Holter monitoring in the non-athletes group was 96 (IQR 68–98) and 98 in the athletes group (IQR 92–99) (p = 0.08). Considering the athletes, the median percentage of decrease in the number of PVCs was 98 (IQR 93–99) and 98 (IQR 87–99), respectively, in leisure-time and agonistic athletes (p = 0.42). Sixteen (70%) leisure time and seventeen (90%) agonist athletes (p = 0.24) have resumed physical activity 3 months after PVC CA; among agonistic athletes, 59% have resumed competitive physical activity. Many leisure-time (88%) and agonist (70%) athletes experienced an improvement in symptoms after ablation. PVC CA was effective and safe in both groups, reducing symptoms and allowing a quick and safe return to sports activities in athletes.
Is Metformin Use Associated with a More Favorable COVID-19 Course in People with Diabetes?
Authors: Silverii Giovanni Antonio , Fumagalli Carlo , Rozzini Renzo , Milani Marta , Mannucci Edoardo , Marchionni Niccolò
Published: March 2024

Background: Diabetes Mellitus (DM) has been associated with a higher Coronavirus disease-19 (COVID-19) mortality, both in hospitalized patients and in the general population. A possible beneficial effect of metformin on the prognosis of COVID-19 has been reported in some observational studies, whereas other studies disagree. Methods: To investigate the possible effect of metformin on COVID-19 in-hospital mortality, we performed a retrospective study that included all SARS-CoV-2-positive patients with DM who were admitted to two Italian hospitals. In order to adjust for possible confounders accounting for the observed reduction of mortality in metformin users, we adopted the COVID-19 Mortality Risk Score (COVID-19 MRS) as a covariate. Results: Out of the 524 included patients, 33.4% died. A binomial logistic regression showed that metformin use was associated with a significant reduction in case fatality (OR 0.67 [0.45–0.98], p = 0.039), with no significant effect on the need for ventilation (OR 0.75 [0.5–1.11], p = 0.146). After adjusting for COVID-19 MRS, metformin did not retain a significant association with in-hospital mortality [OR 0.795 (0.495–1.277), p = 0.342]. Conclusions: A beneficial effect of metformin on COVID-19 was not proven after adjusting for confounding factors. The use of validated tools to stratify the risk for COVID-19 severe disease and death, such as COVID-19 MRS, may be useful to better explore the potential association of medications and comorbidities with COVID-19 prognosis.
The Efficacy of Elonide Nasal Corticosteroids in Managing Allergic Rhinitis: A Randomized, Double-Blinded Trial
Authors: Gendeh Hardip S. , Hamizan Aneeza W. , Husain Salina , Nawi Azmawati M. , Zahedi Farah D. , Megat Ismail Nur Fadhilah , M. Farit N. Ammal
Published: March 2024

Background: Mometasone furoate nasal spray is efficacious in relieving allergic rhinitis symptoms. The objectives of this study were, firstly, to compare the efficacy of Elonide to Nasonex® and a placebo and secondly, to investigate the side effects of Elonide. Method: This was a prospective, single-centered, double blinded, randomized, placebo-controlled, non-inferiority trial. A total of 163 participants from the Otorhinolaryngology Clinic, Hospital Canselor Tuanku Muhriz (HCTM), were randomized into three treatment groups receiving Elonide (n = 56), Nasonex® (n = 54), and placebo (n = 53) nasal sprays using an online randomizer (Random.org). Treatment was administered for 4 weeks. The primary outcome measure was the Total Nasal Resistance (TNR), and the secondary outcomes were the Visual Analogue Score (VAS) and the Rhinoconjunctivitis Quality of Life Questionnaire (RQOLQ) score. Side effects were recorded. Results: There were significant improvements for all groups from baseline. The Elonide group had the greatest mean difference for all primary and secondary outcomes compared to Nasonex® and the placebo (0.77 ± 2.44 vs. 0.35 ± 1.16, p = 1.00 vs. 0.17 ± 0.82, p = 0.01). Elonide is non-inferior to Nasonex (p = 1.00) and superior to the placebo (p < 0.05). The highest side effects reported were for Nasonex (n = 14, 26%), followed by the placebo (n = 8, 16%) and Elonide (n = 6, 12%); headaches (n = 9, 17%) and sore throat (n = 9, 17%) were the most common. Conclusions: Elonide has similar efficacy to Nasonex® when compared to a placebo in the treatment of AR in adults. Elonide is safe and tolerable, with fewer side effects and no adverse side effects.
The Evolving Field of Acute Coronary Syndrome Management: A Critical Appraisal of the 2023 European Society of Cardiology Guidelines for the Management of Acute Coronary Syndrome
Authors: Licordari Roberto , Costa Francesco , Garcia-Ruiz Victoria , Mamas Mamas A. , Marquis-Gravel Guillaume , de la Torre Hernandez Jose M. , Gomez Doblas Juan Jose , Jimenez-Navarro Manuel , Rodriguez-Capitan Jorge , Urbano-Carrillo Cristobal , Ortega-Paz Luis , Piccolo Raffaele , Versace Antonio Giovanni , Di Bella Gianluca , Andò Giuseppe , Angiolillo Dominick J. , Valgimigli Marco , Micari Antonio
Published: March 2024

Acute coronary syndromes (ACS), encompassing conditions like ST-elevation myocardial infarction (STEMI) and non-ST-elevation acute coronary syndromes (NSTE-ACS), represent a significant challenge in cardiovascular care due to their complex pathophysiology and substantial impact on morbidity and mortality. The 2023 European Society of Cardiology (ESC) guidelines for ACS management introduce several updates in key areas such as invasive treatment timing in NSTE-ACS, pre-treatment strategies, approaches to multivessel disease, and the use of imaging modalities including computed tomography (CT) coronary angiography, magnetic resonance imaging (MRI), and intracoronary imaging techniques, such as optical coherence tomography (OCT) and intravascular ultrasound (IVUS). They also address a modulation of antiplatelet therapy, taking into consideration different patient risk profiles, and introduce new recommendations for low-dose colchicine. These guidelines provide important evidence-based updates in practice, reflecting an evolution in the understanding and management of ACS, yet some potentially missed opportunities for more personalized care and technology adoption are discussed.
A Prospective Observational Study on Gastric Endoscopic Submucosal Dissection under Continuous Administration of Antithrombotic Agents
Authors: Kawai Daisuke , Iwamuro Masaya , Takenaka Ryuta , Obata Taisuke , Yamamoto Takashi , Hirata Shoichiro , Miura Ko , Takemoto Koji , Tsugeno Hirofumi , Fujiki Shigeatsu
Published: March 2024

Background: This study aimed to assess the completion rate and postoperative bleeding incidence of endoscopic submucosal dissection (ESD) for gastric tumors under continuous antithrombotic therapy. Methods: A prospective observational study was conducted including 88 patients with 100 gastric lesions who underwent gastric endoscopic submucosal dissection (ESD) and received continuous antithrombotic therapy. Additionally, retrospective data on gastric ESD in 479 patients with 534 lesions who did not receive antithrombotic therapy were collected for comparison. Results: The en bloc resection rates (100% in the continuous antithrombotic therapy group vs. 100% in the non-antithrombotic therapy group) and complete resection rates (97.0% vs. 96.3%, respectively) were high and comparable between the groups. No significant differences were found in the specimen size or procedure time. Perforation rates were low (0% vs. 2.3%, respectively) and were not significantly different between the groups. However, postoperative bleeding occurred significantly more frequently in the continuous antithrombotic therapy group (10.2% vs. 4.2%, respectively) than in the non-antithrombotic therapy group. The subgroup analysis revealed a higher incidence of postoperative bleeding in patients receiving thienopyridine derivatives. Conclusions: Continuous administration of antithrombotic agents, especially thienopyridines, increased the risk of postprocedural hemorrhage following gastric ESD. These findings support the need for careful consideration of pharamcological management before ESD, aligning with the current guidelines.
Exploring the Impact of Novel Anti-Cancer Therapies on Jaw Osteonecrosis and Other Bones: A Comprehensive Review
Authors: Konarski Wojciech , Poboży Tomasz , Konarska Klaudia , Śliwczyński Andrzej , Kotela Ireneusz , Krakowiak Jan
Published: March 2024

Osteonecrosis is a debilitating condition characterized by the loss of blood supply to the bones, leading to bone death. This condition can impact various bones, including the jaw, which significantly affects patients’ quality of life by causing difficulties in swallowing, feeding, chewing, and speaking, along with swollen, painful mucous membranes and chronic sinusitis. Osteonecrosis can arise due to treatment with antiresorptive drugs. However, there is a growing number of reports of osteonecrosis following novel targeted anti-cancer treatments, such as tyrosine kinase inhibitors (TKIs) and biological therapies. The pathogenesis of osteonecrosis is linked to the side effects of the antiangiogenic mechanisms of these medications, leading to a disrupted blood flow. Our review aims to examine recent insights into osteonecrosis triggered by new anti-cancer drugs. Most reports focus on the osteonecrosis of the jaw (ONJ); however, we discovered that some authors have described cases of osteonecrosis affecting the femoral head or elbow following novel anti-cancer treatments. Prevention is a key component in managing osteonecrosis. Therefore, a comprehensive risk assessment should always be performed before and during anti-cancer therapy.
Approach to Liver Transplantation: Is There a Difference between East and West?
Authors: Ozturk Nazli Begum , Bartosek Nathanial , Toruner Merih Deniz , Mumtaz Aymen , Simsek Cem , Dao Doan , Saberi Behnam , Gurakar Ahmet
Published: March 2024

Liver transplantation (LT) remains the only curative treatment for end-stage liver disease as well as acute liver failure. With the exponential increase in organ demand due to the increasing incidence and prevalence of liver diseases, the need to overcome the supply and demand mismatch has arisen. In this review, we discuss the current universal status of LT, emphasizing various LT practices worldwide.
Rosuvastatin-Based Lipid-Lowering Therapy for the Control of LDL Cholesterol in Patients at High Vascular Risk
Authors: Mostaza Jose María , Escobar Carlos
Published: March 2024

Vascular diseases are the leading cause of death in Spain. Hypercholesterolemia is not only a cardiovascular risk factor, but also underlies the etiopathogenesis of atherosclerosis. Therefore, reducing LDL cholesterol (LDL-C) to the goals recommended by clinical practice guidelines, is essential to decrease the risk of vascular complications. Despite this, current LDL-C control is scarce, even in subjects with high and very high risk. This is mainly due to an insufficient intensification of lipid-lowering treatment. In this context, it is essential to prescribe the appropriate therapy, adjusted to patient’s needs based on their LDL-C and their vascular risk. Rosuvastatin, alone or in combination with ezetimibe, provides intensive LDL-C reductions (up to 50–55% and 60–75%, respectively), with a low risk of side effects and in an efficient manner, in patients both without and with established atherosclerotic vascular disease.
Predicting Time to Delivery in Hypertensive Disorders: Assessing PlGF and sFlt-1 with the Novel Parameter ‘Mtp-Multiples of a Normal Term Placenta’
Authors: Giardini Valentina , Santagati Alice Angela Francesca , Marelli Elisabetta , Casati Marco , Cantarutti Anna , Vergani Patrizia
Published: March 2024

Background: Imbalanced angiogenesis is characteristic of normal placental maturation but it also signals placental dysfunction, underlying hypertensive disorders during pregnancy. This study aimed to investigate the relationship between angiogenic placental aging, measured by markers placental growth factor (PlGF) and soluble fms-like tyrosine kinase-1 (sFlt-1) using the new index “Multiples of a normal term placenta” (Mtp) and the duration of pregnancy. Methods: A retrospective observational study was conducted, including singleton pregnancies diagnosed or suspected of hypertensive disorders after the 20th gestational week. Mtp measures how far a single dosage of angiogenic marker deviates from the expected value in an uncomplicated full-term pregnancy (Mpt = sFlt-1/sFlt-1 reference value or PIGF/PIGF reference value). We considered the 90th, 95th, and 97.5th centiles for sFlt-1 and the 2.5th, 5th, and 10th centiles for PlGF as references. Results: The categories with longer time to delivery, regardless of gestational age, were: Mtp PlGF 10th c ≥ 2, ≥3 and Mtp sFlt-1 90th c ≤ 0.5 (median days of 9, 11, 15 days, respectively). These two categories Mtp sFlt-1 90th c ≥ 3 and Mtp sFlt-1 97.5th c ≥ 2 allow the identification of women at risk for imminent delivery within 1 day. Women who were deemed at low/medium risk based on the sFlt-1/PIGF ratio appeared to be at high risk when considering the individual values of sFlt-1 and/or PIGF. Conclusions: This new Mtp index for sFlt-1 and PlGF could be employed to assess the degree of placental aging in women with hypertensive disorders. It represents a valid tool for evaluating the risk of imminent birth, irrespective of gestational age, surpassing the current stratification based on the sFlt-1/PIGF ratio.
A Compact 0.73~3.1 GHz CMOS VCO Based on Active-Inductor and Active-Resistor Topology
Authors: Pakasiri Chatrpol , Hsu Ke-Chung , Wang Sen
Published: March 2024

In this paper, a wideband VCO that covers popular Long-Term Evolution (LTE) 0.7 GHz and LTE 2.6 GHz frequencies is designed and developed in a standard 0.18 μm CMOS process. The VCO utilizes active inductors to achieve coarse-tuning of the inductance and a compact chip area. Moreover, an active feedback resistor is introduced into the active inductor for fine-tuning of the inductance. The feedback resistor also affects the equivalent resistance of the active inductor; therefore, wide inductance tuning and low power consumption can be obtained by optimizing the resistor. The core area of the fabricated CMOS chip is merely 0.046 mm2, excluding all testing pads. With a 6.7~10.1 mW DC consumption, the measured oscillation frequencies range from 0.73 GHz to 3.1 GHz, which demonstrates a 123.8% tuning range. At the frequencies of interest, the measured phase noises are from −80.7 to −84.5 dBc/Hz at a 1 MHz offset frequency.
Effect of Internal Waves on the Hydrodynamics of a Mediterranean Sea Strait
Authors: Fourniotis Nikolaos Th.
Published: March 2024

In the present work, the effects of wind- and tide-induced internal waves in the Rio-Antirio Strait in western Greece were studied by using three-dimensional numerical simulations. For the wind-induced flow in the strait, it emerged that the internal waves’ initiation is associated with the direction of the wind. Tidal action, with or without the combined action of wind, also generates internal waves in the strait, with amplitudes higher than 20 m. The action of the internal waves causes a subsurface inflow of colder waters from the Gulf of Corinth to the Gulf of Patras, as has been also simulated for the case of the wind-induced flow, generating strong hypolimnetic currents. The exchange flowrate between the Gulf of Patras and the Gulf of Corinth appeared to undergo significant modification for the wind-induced flow and had little effect for the pure tidal flow (in windless conditions) due to the development and action of the internal waves at the strait. The combined action of the tide and the wind was found to marginally affect the exchange flowrate between the two gulfs compared to the pure tidal flow. The interaction between the Coriolis effect and internal waves, at least away from the strait, forms a characteristic horizontal structure of flow. The structure of turbulence in the near strait area under the action of internal waves generated by the wind and/or tide was also discussed and compared with the corresponding barotropic flow.
Short-Term Exhaust Gas Temperature Trend Prediction of a Marine Diesel Engine Based on an Improved Slime Mold Algorithm-Optimized Bidirectional Long Short-Term Memory—Temporal Pattern Attention Ensemble Model
Authors: Sun Jianping , Zeng Hong , Ye Kailun
Published: March 2024

As the core component of a ship’s engine room, the operation of a marine diesel engine (MDE) directly affects the economy and safety of the entire vessel. Predicting the future changes in the status parameters of a MDE helps to understand the operational status, enabling timely warnings to the engine crew, and to ensure the safe navigation of the vessel. Therefore, this paper combines the temporal pattern attention mechanism with the bidirectional long short-term memory (BiLSTM) network to propose a novel trend prediction method for short-term exhaust gas temperature (EGT) forecasting. First, the Pearson correlation analysis (PCA) is conducted to identify input feature variables that are strongly correlated with the EGT. Next, the BiLSTM network models input feature variables such as load, fuel oil pressure, and scavenging air pressure and capture the interrelationships between different vectors from the hidden layer matrix within the BiLSTM network. This allows the selection of valuable information across different time steps. Meanwhile, the temporal pattern attention (TPA) mechanism has the ability to explore complex nonlinear dependencies between different time steps and series. This assigns appropriate weights to the feature variables within different time steps of the BiLSTM hidden layer, thereby influencing the input effect. Finally, the improved slime mold algorithm (ISMA) is utilized to optimize the hyperparameters of the prediction model to achieve the best level of short-term EGT trend prediction performance based on the ISMA-BiLSTM-TPA model. The prediction results show that the mean square error, the mean absolute percentage error, the root mean square error and the coefficient of determination of the model are 0.4284, 0.1076, 0.6545 and 98.2%, respectively. These values are significantly better than those of other prediction methods, thus fully validating the stability and accuracy of the model proposed in this paper.
Seafloor Sediment Acoustic Properties on the Continental Slope in the Northwestern South China Sea
Authors: Li Guanbao , Wang Jingqiang , Meng Xiangmei , Hua Qingfeng , Kan Guangming , Liu Chenguang
Published: March 2024

The acoustic properties of seafloor sediments on continental slopes play a crucial role in underwater acoustic propagation, communication, and detection. To investigate the acoustic characteristics and spatial distribution patterns of sediments on the continental slope, a geoacoustic experiment was conducted in the northwestern South China Sea. The experiment covered two sections: one crossing the shelf and slope in the downslope direction, and the other near the shelf break in the along-slope direction. In situ techniques, sediment sampling, and laboratory measurements were used to acquire data on sediment acoustic properties (such as sound speed and attenuation) and physical properties (including particle composition, density, porosity, and mean grain size). The experimental findings revealed several key points: (1) Acoustic properties of shallow water coarse-grained sediments and deep-sea sediments were higher when measured in the laboratory compared to in situ measurements. (2) Relationships between measured attenuation and physical properties, as well as between sound speed and mean grain size, showed deviations from previous empirical equations. (3) Sediment acoustic and physical properties exhibited significant variations in the downslope direction, while showing gradual variations in the along-slope direction. These variations can be attributed to sedimentary environmental factors such as material sources, hydrodynamic conditions, and water depth.
A New Method for the Correction of Spectrum Drift Caused by Temperature Changes When Using a NaI(Tl) Seawater Radioactivity Sensor
Authors: Shi Yan , Zhang Yingying , Feng Xiandong , Yuan Da , Wu Bingwei , Bi Haijie
Published: March 2024

The seawater radioactivity sensor based on the NaI(Tl) scintillation crystal detection method is prone to spectrum drift due to environment temperature changes during actual measurement, which pose difficulties in the activity analysis of seawater radionuclides. This paper adopts experiment design and analysis methods to study the relationship laws between temperature and peak position channel address and between gain and the peak position channel address. On the basis of studying and summarizing the two relationship laws of the NaI(Tl) seawater radioactivity sensor, a spectrum drift correction method combining gain adjustment and spectrum processing is proposed. Laboratory air and water environment experiments verify that the peak position channel address drift after air environment correction does not exceed ±2 channels, and after water environment correction does not exceed ±1 channel. Long-term verification experiments at the seawater work site have shown that this correction method can effectively correct the spectrum drift of the NaI(Tl) seawater radioactivity sensor and meet the requirements of long-term automatic operations at sea for the measured spectrum.
Coupled Dynamic Characteristics of a Spar-Type Offshore Floating Two-Bladed Wind Turbine with a Flexible Hub Connection
Authors: Wu Zonghao , Wang Kai , Jie Tianyu , Wu Xiaodi
Published: March 2024

To reduce manufacturing, transportation, lifting and maintenance costs of increasingly larger and larger floating wind turbines, a Spar-type floating two-bladed wind turbine based on the 5 MW OC3-Hywind floating wind turbine model from the National Renewable Energy Laboratory (NREL) is studied in this paper. The two-bladed wind turbine can cause serious problems with large dynamic loads, so a flexible hub connection was introduced between the hub mount and nacelle carrier to alleviate the dynamic effect. The paper focuses on studying the dynamic responses of the proposed Spar-type floating two-bladed wind turbine with a flexible hub connection at rated and extreme environmental conditions. Fully coupled time-domain simulations are carried out by integrating aerodynamic loads on blades, hydrodynamic loads on the spar, structural dynamics of the tower, blades and mooring lines, control system and flexible hub connection. The analysis results show that the application of a flexible hub connection between the hub mount and nacelle carrier can make a contribution to enable the Spar-type floating two-bladed wind turbine to effectively dampen the motion of the floating platform, while significantly reducing the tower load and blade deflection.
Superficial Zoonotic Mycoses in Humans Associated with Cats
Authors: Piorunek Marcin , Kubisiak-Rzepczyk Honorata , Dańczak-Pazdrowska Aleksandra , Trafas Tomasz , Walkowiak Jarosław
Published: March 2024

Dermatophytosis is a superficial fungal skin infection common in humans around the world and is one of the many zoonotic skin diseases that cat owners are at risk of contracting. This retrospective study was conducted based on a detailed analysis of the results of mycological examination and medical documentation of 56 patients diagnosed with cat-to-human dermatophytoses from January 2017 to July 2022. Zoonotic mycoses were diagnosed more frequently in young people and women. In children, lesions most often occurred in the scalp area, and in adults, in the glabrous skin area. Skin infections caused by Microsporum canis (M. canis) prevailed and were confirmed in 47 patients (83.9%). Trichophyton mentagrophytes (T. mentagrophytes) was found in nine (16.1%) patients. M. canis predominantly caused infections of the scalp, followed by lower limb infections. Hairy scalps were almost exclusively involved in children. The odds of diagnosing M. canis infection compared to T. mentagrophytes infection was significantly higher in the head than in other regions, especially among children. The positive predictive value of a direct macroscopic examination was relatively low.
Reference Values for the German Version of the Quality of Life after Brain Injury in Children and Adolescents (QOLIBRI-KID/ADO) from a General Population Sample
Authors: Krol Leonie , Hagmayer York , Steinbuechel Nicole von , Cunitz Katrin , Buchheim Anna , Koerte Inga K. , Zeldovich Marina
Published: March 2024

Assessment of health-related quality of life (HRQoL) after pediatric traumatic brain injury (TBI) has been limited in children and adolescents due to a lack of disease-specific instruments. To fill this gap, the Quality of Life after Traumatic Brain Injury for Children and Adolescents (QOLIBRI-KID/ADO) Questionnaire was developed for the German-speaking population. Reference values from a comparable general population are essential for comprehending the impact of TBI on health and well-being. This study examines the validity of the German QOLIBRI-KID/ADO in a general pediatric population in Germany and provides reference values for use in clinical practice. Overall, 1997 children and adolescents aged 8–17 years from the general population and 300 from the TBI population participated in this study. The questionnaire was tested for reliability and validity. A measurement invariance (MI) approach was used to assess the comparability of the HRQoL construct between both samples. Reference values were determined by percentile-based stratification according to factors that significantly influenced HRQoL in regression analyses. The QOLIBRI-KID/ADO demonstrated strong psychometric properties. The HRQoL construct was measured largely equivalently in both samples, and reference values could be provided. The QOLIBRI-KID/ADO was considered reliable and valid for assessing HRQoL in a general German-speaking pediatric population, allowing for clinically meaningful comparisons between general and TBI populations.
Prevalence of Peri-Implant Mucositis, Peri-Implantitis and Associated Risk Indicators of Implants with and without Laser-Microgrooved Collar Surface: A Long-Term (≥20 Years) Retrospective Study
Authors: Guarnieri Renzo , Reda Rodolfo , Di Nardo Dario , Pagnoni Francesco , Zanza Alessio , Testarelli Luca
Published: March 2024

The aim of the current study was to retrospectively investigate the prevalence of peri-implant mucositis (PIM) and peri-implantitis (P) in a long-term follow-up (≥20 years) of implants with the same body design and body surface but different collar surfaces with laser-microtextured grooves (LMGSs) vs. no laser-microtextured grooves (no-LMGSs) in private practice patients. Furthermore, several patient-related, implant-related, site-, surgical-, and prosthesis-related potential disease risk factors were analyzed. A chart review of patients receiving at least one pair of implants (one with an LMGS and the other without LMGS) in the period 1993–2002 was used. Chi-square analysis was used to determine if a statistically significant difference between the investigated variables and PIM/P was present. Possible risk factors were statistically evaluated by a binary logistic regression analysis. A total of 362 patients with 901 implant-supported restorations (438 with LMGS and 463 no-LMGS) were included in the study. The cumulative survival rates of implants at 5, 10, 15, and 20 years were 98.1%, 97.4%, 95.4%, and 89.8%, respectively, for the LMGS group, and 93.2%, 91.6%, 89.5%, and 78.3% for the no-LMGS group. The difference was statistically significant at all timepoints (p < 0.05). In total, at the end of the follow-up period, 45.7% of patients and 39.8% of implants presented PIM, and 15.6% of patients and 14% of implants presented P. A total of 164 LMGS implants (37.4%) and 195 no-LMGS implants (42.1%) presented peri-implant mucositis, while 28 (6.3%) of LMGS implants and 98 (21.1%) no-LMGS implants demonstrated peri-implantitis. Differences between LMGS implants and no-LMGS implants were statistically significant (p < 0.05). The binary logistic regression identified collar surface, cigarette smoking, histories of treated periodontitis, and lack of peri-implant maintenance as risk factors for P. After at least 20 years of function in patients followed privately, LMGS implants compared to no-LMGS implants presented a statistically and significantly lower incidence of P. Implant collar surface, cigarette smoking, previously treated periodontitis, and lack of peri-implant maintenance are factors with significant association to P.
The Index of the Cycle of Money: The Case of Switzerland
Authors: Challoumis Constantinos
Published: March 2024

This article focuses on the study of issues related to the functionality and structure of an economy. To achieve this, the theory of the cycle of money is used. The structural features of an economy are reflected in its operational characteristics, and vice versa. The index of the cycle of money indexes how well an economic system can counteract a financial crisis and characterizes how well structured a country’s economy is. Calculations of the index of the cycle of money in Switzerland were compared with the global average index. The results showed that Switzerland is close to the global average; therefore, it has an excellent economy and is equipped to face any economic crisis. The applied methodology abides by theoretical, mathematical, statistical, and econometrical outcomes. This work is significant as it demonstrates the strength of Switzerland’s economy in response to a potential crisis. Prior case studies were reviewed from Latvia, Bulgaria, Serbia, Thailand, Greece, Montenegro, and many other countries. This study postulates that companies with high capital should invest in manufacturing and high technology sectors that should be subject to fewer taxes; this approach facilitates a better distribution of money to the economy by allowing small companies to service the remaining economic activities. The period used for compilations in this study was the global recession of 2007–2017. The reviewed case study results are from a project studying multiple countries, and at present, this article presents the only study about Switzerland’s index of the cycle of money.