bluuebunny/crossref_metadata_2025 huggingface dataset:
Factors Affecting Utilisation of Indigenous Knowledge to Control Gastrointestinal Nematodes in Goats
Authors: Ndlela Sithembile Z. , Mkwanazi Mbusiseni V. , Chimonyo Michael
Published: February 2021

Adoption and utilisation of indigenous knowledge (IK) is declining. The objective of the current study was to determine differences in the extent of use of IK to control gastrointestinal nematodes (GIN) in goats between wet and dry environments. A structured questionnaire was used to collect data. Almost all households used IK in controlling parasites. There was a close association among environment, gender, and religion (p < 0.05) on IK use. Farmers who were less poor were 2.38 times more likely to use IK (p > 0.05) than farmers that were poor. Adults were 1.20 more likely to use IK (p < 0.05) than younger people. Unemployed farmers were 4.26 more likely to use IK compared to their employed counter parts (p < 0.01). Having a herbalist in the community was 3.6 times more likely to influence the use of IK (p < 0.05) compared to the environment in which there was no herbalist. Farmers that received informal education in the dry environment were 5.88 times more likely to use IK (p < 0.05) than those in the wet environment. Farmers who practised traditional Zulu culture were 2.05 times more likely to use IK compared to those following the Christian faith (p < 0.05). The considerable variation in the adoption of IK suggests that intervention strategies that advance IK use should consider the socio-demographic information of the community.
Clinical Efficacy of Doxycycline for Treatment of Macrolide-Resistant Mycoplasma pneumoniae Pneumonia in Children
Authors: Lee Hyunju , Choi Youn Young , Sohn Young Joo , Kim Ye Kyung , Han Mi Seon , Yun Ki Wook , Kim Kyungmin , Park Ji Young , Choi Jae Hong , Cho Eun Young , Choi Eun Hwa
Published: February 2021

In areas with high prevalence of macrolide-resistant Mycoplasma pneumoniae (MRMP) pneumonia, treatment in children has become challenging. This study aimed to analyze the efficacy of macrolides and doxycycline with regard to the presence of macrolide resistance. We analyzed children with MP pneumonia during the two recent epidemics of 2014–2015 and 2019–2020 from four hospitals in Korea. Nasopharyngeal samples were obtained from children with pneumonia for MP cultures and polymerase chain reaction (PCR). Macrolide resistance was determined by the analysis of 23S rRNA gene transition. Time to defervescence and to chest X-ray improvement were analyzed. Of 145 cases, the median age was 5.0 years and MRMP accounted for 59 (40.7%). Among macrolide-susceptible MP (MSMP), 78 (90.7%) were treated with macrolides and 21 (35.6%) in the MRMP group with doxycycline. In MRMP pneumonia, shorter days to defervescence (2 vs. 5 days, p < 0.001) and to chest X-ray improvement (3 vs. 6 days, p < 0.001) in the doxycycline group than in the macrolide group was observed, whereas no differences were observed among children with MSMP pneumonia. Compared to macrolides, treatment with doxycycline resulted in better outcomes with a shorter time to defervescence and to chest X-ray improvement among children with MRMP pneumonia.
Unleashing the Biological Potential of Fomes fomentarius via Dry and Wet Milling
Authors: Darkal Abdul Karim , Zuraik Mhd Mouayad , Ney Yannick , Nasim Muhmmad Jawad , Jacob Claus
Published: February 2021

Fomes fomentarius, usually referred to as tinder conk, is a common wood-based fungus rich in many interesting phytochemicals and with an unique porous structure. Dry or wet ball milling of this sponge on a planetary mill results in small particles with sizes in the range of 10 µm or below. Suspended in water and without preservatives or other stabilizers, the resulting micro-suspensions are sterile for around six days, probably due to the increased temperatures of around 80 °C especially during the wet milling process. The suspensions also exhibit excellent antioxidant activities as determined in the DPPH, ferric reducing antioxidant potential (FRAP) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assays. In the DPPH assay, IC50 values of 0.02–0.04% w/v and 0.04% w/v were observed for dry and wet milled samples, respectively. In the FRAP assay, IC50 values of <0.02% w/v and 0.04% w/v were observed for dry and wet milled samples, respectively. In contrast, the ABTS assay provided IC50 values of 0.04% w/v and 0.005% w/v, respectively. Notably, this activity is mostly—albeit not exclusively—associated with the highly porous particles and their large surfaces, although some active ingredients also diffuse into the surrounding aqueous medium. Such suspensions of natural particles carrying otherwise insoluble antioxidants on their surfaces provide an interesting avenue to unleash the antioxidant potential of materials such as sponges and barks. As dry milling also enables longer storage and transport, applications in the fields of medicine, nutrition, agriculture, materials and cosmetics are feasible.
Onion Peel: Turning a Food Waste into a Resource
Authors: Celano Rita , Docimo Teresa , Piccinelli Anna Lisa , Gazzerro Patrizia , Tucci Marina , Di Sanzo Rosa , Carabetta Sonia , Campone Luca , Russo Mariateresa , Rastrelli Luca
Published: February 2021

Food waste is a serious problem for food processing industries, especially when it represents a loss of a valuable source of nutrients and phytochemicals. Increasing consumer demand for processed food poses the problem of minimizing waste by conversion into useful products. In this regard, onion (Allium cepa) waste consisting mainly of onion skin is rich in bioactive phenolic compounds. Here, we characterized the flavonoid profiles and biological activities of onion skin wastes of two traditional varieties with protected geographical indication (PGI), the red “Rossa di Tropea” and the coppery “Ramata di Montoro”, typically cultivated in a niche area in southern Italy. The phytochemical profiles of exhaustive extracts, characterized by ultra-high-performance liquid chromatography coupled with ultraviolet (UV) detection and high-resolution mass spectrometry, revealed that flavonols and anthocyanins were the characteristic metabolite classes of onion skins. Quercetin, quercetin glucosides and their dimer and trimer derivatives, and, among anthocyanins, cyanidin 3-glucoside, were the most abundant bioactive compounds. The potential of onion skins was evaluated by testing several biological activities: ABTS/oxygen radical absorbance capacity (ORAC) and in vitro alpha-glucosidase assays were performed to evaluate the antioxidant and anti-diabetic properties of the extracts and of their main compounds, respectively, and proliferative activity was evaluated by MTT assay on human fibroblasts. In the present study, by observing various biological properties of “Rossa di Tropea” and “Ramata di Montoro” onion-dried skins, we clearly indicated that this agricultural waste can provide bioactive molecules for multiple applications, from industrial to nutraceutical and cosmetical sectors.
Inhibitory Effect of a Rosmarinic Acid-Enriched Fraction Prepared from Nga-Mon (Perilla frutescens) Seed Meal on Osteoclastogenesis through the RANK Signaling Pathway
Authors: Phromnoi Kanokkarn , Suttajit Maitree , Saenjum Chalermpong , Limtrakul (Dejkriengkraikul) Pornngarm
Published: February 2021

The aim of this study is to determine antioxidant and anti-inflammatory activities relating to the antiosteoporosis effects of various perilla seed meal (PSM) fractions. The remaining waste of perilla seed obtained from cold oil compression was extracted with 70% ethanol and sequentially fractionated according to solvent polarity with hexane, dichloromethane, ethyl acetate, and water. The results indicated that the seed-meal ethyl acetate fraction (SMEF) exhibited the highest antioxidant and anti-inflammatory activities, and rosmarinic acid (RA) content. The signaling pathways induced by the receptor activator of the nuclear factor kappa B (NF-κB) ligand (RANKL) that trigger reactive oxygen species (ROS) and several transcription factors, leading to the induction of osteoclastogenesis, were also investigated. The SMEF clearly showed attenuated RANKL-induced tartrate-resistant acid phosphatase (TRAP)-positive multinucleated osteoclasts and TRAP activity. A Western blot analysis showed that the SMEF significantly downregulated RANKL-induced NF-κB, AP-1 activation, and the nuclear factor of activated T-cell 1 (NFATc1) expression. SMEF also suppressed RANKL-induced osteoclast-specific marker gene-like MMP-9 using zymography. Furthermore, the SMEF showed inhibition of RANKL-induced ROS production in RAW 264.7 cells. The results suggest that the SMEF, which contained high quantities of RA, could be developed as a natural active pharmaceutical ingredient for osteoclastogenic protection and health promotion.
Fully Leveraging Deep Learning Methods for Constructing Retinal Fundus Photomontages
Authors: Kim Jooyoung , Go Sojung , Noh Kyoung Jin , Park Sang Jun , Lee Soochahn
Published: February 2021

Retinal photomontages, which are constructed by aligning and integrating multiple fundus images, are useful in diagnosing retinal diseases affecting peripheral retina. We present a novel framework for constructing retinal photomontages that fully leverage recent deep learning methods. Deep learning based object detection is used to define the order of image registration and blending. Deep learning based vessel segmentation is used to enhance image texture to improve registration performance within a two step image registration framework comprising rigid and non-rigid registration. Experimental evaluation demonstrates the robustness of our montage construction method with an increased amount of successfully integrated images as well as reduction of image artifacts.
The Effect of Intensive Dietary Intervention on the Level of RANTES and CXCL4 Chemokines in Patients with Non-Obstructive Coronary Artery Disease: A Randomised Study
Authors: Makarewicz-Wujec Magdalena , Henzel Jan , Kruk Mariusz , Kępka Cezary , Wardziak Łukasz , Trochimiuk Piotr , Parzonko Andrzej , Demkow Marcin , Dzielińska Zofia , Kozłowska-Wojciechowska Malgorzata
Published: February 2021

Background: Inflammation is the key pathophysiological mechanism of the initiation and progression of atherosclerosis. The study objective was to assess the effects of a dietary intervention based on the model of the dietary approaches to stop hypertension (DASH) diet on the levels of chemokines RANTES and CXCL4 in patients with non-obstructive coronary artery disease. Methods: As part of Dietary Intervention to Stop Coronary Atherosclerosis in Computed Tomography (DISCO-CT) study, patients were randomised to an intervention group (n = 40), where the DASH diet was introduced along with optimal pharmacotherapy, and to a control group (n = 39), with optimal pharmacotherapy alone. In the DASH group, systematic dietary counselling was provided for the follow-up period. RANTES and CXCL4 levels were determined using ELISA. Results: In the DASH group, the RANTES level insignificantly reduced from 42.70 ± 21.1 ng/mL to 38.09 ± 18.5 ng/mL (p = 0.134), and the CXCL4 concentration significantly reduced from 12.38 ± 4.1 ng/mL to 8.36 ± 2.3 ng/mL (p = 0.0001). At the same time, an increase in the level of both chemokines was observed in the control group: RANTES from 34.69 ± 22.7 to 40.94 ± 20.0 ng/mL (p = 0.06) and CXCL4 from 10.98 ± 3.6 to 13.0 5± 4.8 ng/mL (p = 0.009). The difference between the changes in both groups was significant for both RANTES (p = 0.03) and CXCL4 (p = 0.00001). The RANTES/CXCL4 ratio reduced in the control group (from 3.52 ± 2.8 to 3.35 ± 2.8; p = 0.006), while in the DASH group, an increase was observed (from 3.54 ± 1.7 to 4.77 ± 2.4; p = 0.001). Conclusions: A 12-month-long intensive dietary intervention based on DASH diet guidelines as an addition to optimal pharmacotherapy causes changes in the levels of chemokines CXCL4 and RANTES and their mutual relationship in comparison to conventional treatment.
Chromochloris zofingiensis (Chlorophyceae) Divides by Consecutive Multiple Fission Cell-Cycle under Batch and Continuous Cultivation
Authors: Koren Idan , Boussiba Sammy , Khozin-Goldberg Inna , Zarka Aliza
Published: February 2021

Several green algae can divide by multiple fission and spontaneously synchronize their cell cycle with the available light regime. The yields that can be obtained from a microalgal culture are directly affected by cell cycle events. Chromochloris zofingiensis is considered as one of the most promising microalgae for biotechnological applications due to its fast growth and the flexible trophic capabilities. It is intensively investigated in the context of bio-commodities production (carotenoids, storage lipids); however, the pattern of cell-cycle events under common cultivation strategies was not yet characterized for C. zofingiensis. In this study, we have employed fluorescence microscopy to characterize the basic cell-cycle dynamics under batch and continuous modes of phototrophic C. zofingiensis cultivation. Staining with SYBR green—applied in DMSO solution—enabled, for the first time, the clear and simple visualization of polynuclear stages in this microalga. Accordingly, we concluded that C. zofingiensis divides by a consecutive pattern of multiple fission, whereby it spontaneously synchronizes growth and cell division according to the available illumination regime. In high-light continuous culture or low-light batch culture, C. zofingiensis cell-cycle was completed within several light-dark (L/D) cycles (14 h/10 h); however, cell divisions were synchronized with the dark periods only in the high-light continuous culture. In both modes of cultivation, daughter cell release was mainly facilitated by division of 8 and 16-polynuclear cells. The results of this study are of both fundamental and applied science significance and are also important for the development of an efficient nuclear transformation system for C. zofingiensis.
Searching for Peptide Inhibitors of T Regulatory Cell Activity by Targeting Specific Domains of FOXP3 Transcription Factor
Authors: Lozano Teresa , Casares Noelia , Martil-Otal Celia , Anega Blanca , Gorraiz Marta , Parker Jonathan , Ruiz Marta , Belsúe Virginia , Pineda-Lucena Antonio , Oyarzabal Julen , Lasarte Juan José
Published: February 2021

(1) Background: The ability of cancer cells to evade the immune system is due in part to their capacity to induce and recruit T regulatory cells (Tregs) to the tumor microenvironment. Strategies proposed to improve antitumor immunity by depleting Tregs generally lack specificity and raise the possibility of autoimmunity. Therefore, we propose to control Tregs by their functional inactivation rather than depletion. Tregs are characterized by the expression of the Forkhead box protein 3 (FOXP3) transcription factor, which is considered their “master regulator”. Its interaction with DNA is assisted primarily by its interaction with other proteins in the so-called “Foxp3 interactome”, which elicits much of the characteristic Treg cell transcriptional signature. We speculated that the disruption of such a protein complex by using synthetic peptides able to bind Foxp3 might have an impact on the functionality of Treg cells and thus have a therapeutic potential in cancer treatment. (2) Methods: By using a phage-displayed peptide library, or short synthetic peptides encompassing Foxp3 fragments, or by studying the crystal structure of the Foxp3:NFAT complex, we have identified a series of peptides that are able to bind Foxp3 and inhibit Treg activity. (3) Results: We identified some peptides encompassing fragments of the leuzin zipper or the C terminal domain of Foxp3 with the capacity to inhibit Treg activity in vitro. The acetylation/amidation of linear peptides, head-to-tail cyclization, the incorporation of non-natural aminoacids, or the incorporation of cell-penetrating peptide motifs increased in some cases the Foxp3 binding capacity and Treg inhibitory activity of the identified peptides. Some of them have shown antitumoral activity in vivo. (4) Conclusions: Synthetic peptides constitute an alternative to inhibit Foxp3 protein–protein interactions intracellularly and impair Treg immunosuppressive activity. These peptides might be considered as potential hit compounds on the design of new immunotherapeutic approaches against cancer.
Anti-Metastatic and Anti-Inflammatory Effects of Matrix Metalloproteinase Inhibition by Ginsenosides
Authors: Lee Sang Yeol
Published: February 2021

Matrix metalloproteinases (MMPs) are proteolytic enzymes which cleave extracellular matrix (ECM) and other substrates. They are deeply involved in both cancer metastasis and human chronic inflammatory diseases such as osteoarthritis and Crohn’s disease. Regulation of MMPs is closely associated with signaling molecules, especially mitogen-activated protein kinases (MAPKs), including three representative kinases, extracellular signal regulated kinases (ERK), p38 and c-Jun N-terminal kinases (JNK). Ginseng (Panax sp.) is a plant which has been traditionally used for medicinal applications. Ginsenosides are major metabolites which have potentials to treat various human diseases. In this review, the pharmacological effects of ginsenosides have been rigorously investigated; these include anti-metastatic and anti-inflammatory activities of ginsenosides associated with suppression of MMPs via regulation of various signaling pathways. This will highlight the importance of MMPs as therapeutic targets for anti-metastatic and anti-inflammatory drug development based on ginsenosides.
The Focal Attention Window Size Explains Letter Substitution Errors in Reading
Authors: Daini Roberta , Primativo Silvia , Albonico Andrea , Veronelli Laura , Malaspina Manuela , Corbo Massimo , Martelli Marialuisa , Arduino Lisa S.
Published: February 2021

Acquired Neglect Dyslexia is often associated with right-hemisphere brain damage and is mainly characterized by omissions and substitutions in reading single words. Martelli et al. proposed in 2011 that these two types of error are due to different mechanisms. Omissions should depend on neglect plus an oculomotor deficit, whilst substitutions on the difficulty with which the letters are perceptually segregated from each other (i.e., crowding phenomenon). In this study, we hypothesized that a deficit of focal attention could determine a pathological crowding effect, leading to imprecise letter identification and consequently substitution errors. In Experiment 1, three brain-damaged patients, suffering from peripheral dyslexia, mainly characterized by substitutions, underwent an assessment of error distribution in reading pseudowords and a T detection task as a function of cue size and timing, in order to measure focal attention. Each patient, when compared to a control group, showed a deficit in adjusting the attentional focus. In Experiment 2, a group of 17 right-brain-damaged patients were asked to perform the focal attention task and to read single words and pseudowords as a function of inter-letter spacing. The results allowed us to confirm a more general association between substitution-type reading errors and the performance in the focal attention task.
HIV Infection and Related Mental Disorders
Authors: Nosik Marina , Lavrov Vyacheslav , Svitich Oxana
Published: February 2021

Over the more than thirty-year period of the human immunodeficiency virus type 1 (HIV-1) epidemic, many data have been accumulated indicating that HIV infection predisposes one to the development of mental pathologies. It has been proven that cognitive disorders in HIV-positive individuals are the result of the direct exposure of the virus to central nervous system (CNS) cells. The use of antiretroviral therapy has significantly reduced the number of cases of mental disorders among people infected with HIV. However, the incidence of moderate to mild cognitive impairment at all stages of HIV infection is still quite high. This review describes the most common forms of mental pathology that occur in people living with HIV and presents the current concepts on the possible pathogenetic mechanisms of the influence of human immunodeficiency virus (HIV-1) and its viral proteins on the cells of the CNS and the CNS’s functions. This review also provides the current state of knowledge on the impact of the antiretroviral therapy on the development of mental pathologies in people living with HIV, as well as current knowledge on the interactions between antiretroviral and psychotropic drugs that occur under their simultaneous administration.
p38 MAPK Inhibition Mitigates Hypoxia-Induced AR Signaling in Castration-Resistant Prostate Cancer
Authors: Cheung Serina , Jain Pallavi , So Jonathan , Shahidi Saeid , Chung Stephen , Koritzinsky Marianne
Published: February 2021

Background: Aberrant androgen receptor (AR) signaling is a major driver of castration-resistant prostate cancer (CRPC). Tumor hypoxia increases AR signaling and is associated with treatment resistance in prostate cancer. Heat shock protein 27 (Hsp27) is a molecular chaperone that is activated in response to heat shock and hypoxia. Hsp27 has previously been reported to facilitate AR nuclear translocation in a p38 mitogen-activated protein kinase (MAPK) dependent manner in castration-sensitive prostate cancer cell lines. Here, we evaluated the potential for inhibiting p38 MAPK/Hsp27 mediated AR signaling under normoxia and hypoxia in experimental models of CRPC. Methods: We inhibited p38 MAPK with SB203580 in prostate cancer cell lines and measured Hsp27 phosphorylation, AR activity, cell proliferation, and clonogenicity under normoxia and hypoxia. AR activity was measured using an androgen response element driven reporter assay and qPCR to measure expression of AR target genes. Xenograft-bearing mice were treated with SB203580 to measure tumor growth and serum prostate specific antigen (PSA). Results: Our results indicate that p38 MAPK and Hsp27 are activated under normoxia and hypoxia in response to androgens in CRPC cells. p38 MAPK inhibition diminished Hsp27 activation and the hypoxia-mediated increase in AR activity. Additionally, inhibition of p38 MAPK activity decreased proliferation and survival of CRPC cells in vitro and prolonged the survival of tumor-bearing mice. Conclusions: These results suggest that p38 MAPK inhibition may represent a therapeutic strategy to disrupt AR signaling in the heterogeneous CRPC tumor microenvironment.
Eosinophil Lineage-Committed Progenitors as a Therapeutic Target for Asthma
Authors: Salter Brittany M. , Ju Xiaotian , Sehmi Roma
Published: February 2021

Eosinophilic asthma is the most prevalent phenotype of asthma. Although most asthmatics are adequately controlled by corticosteroid therapy, a subset (5–10%) remain uncontrolled with significant therapy-related side effects. This indicates the need for a consideration of alternative treatment strategies that target airway eosinophilia with corticosteroid-sparing benefits. A growing body of evidence shows that a balance between systemic differentiation and local tissue eosinophilopoietic processes driven by traffic and lung homing of bone marrow-derived hemopoietic progenitor cells (HPCs) are important components for the development of airway eosinophilia in asthma. Interleukin (IL)-5 is considered a critical and selective driver of terminal differentiation of eosinophils. Studies targeting IL-5 or IL-5R show that although mature and immature eosinophils are decreased within the airways, there is incomplete ablation, particularly within the bronchial tissue. Eotaxin is a chemoattractant for mature eosinophils and eosinophil-lineage committed progenitor cells (EoP), yet anti-CCR3 studies did not yield meaningful clinical outcomes. Recent studies highlight the role of epithelial cell-derived alarmin cytokines, IL-33 and TSLP, (Thymic stromal lymphopoietin) in progenitor cell traffic and local differentiative processes. This review provides an overview of the role of EoP in asthma and discusses findings from clinical trials with various therapeutic targets. We will show that targeting single mediators downstream of the inflammatory cascade may not fully attenuate tissue eosinophilia due to the multiplicity of factors that can promote tissue eosinophilia. Blocking lung homing and local eosinophilopoiesis through mediators upstream of this cascade may yield greater improvement in clinical outcomes.
Oligomeric Aβ1-42 Induces an AMD-Like Phenotype and Accumulates in Lysosomes to Impair RPE Function
Authors: Lynn Savannah A. , Johnston David A. , Scott Jenny A. , Munday Rosie , Desai Roshni S. , Keeling Eloise , Weaterton Ruaridh , Simpson Alexander , Davis Dillon , Freeman Thomas , Chatelet David S. , Page Anton , Cree Angela J. , Lee Helena , Newman Tracey A. , Lotery Andrew J. , Ratnayaka J. Arjuna
Published: February 2021

Alzheimer’s disease-associated amyloid beta (Aβ) proteins accumulate in the outer retina with increasing age and in eyes of age-related macular degeneration (AMD) patients. To study Aβ-induced retinopathy, wild-type mice were injected with nanomolar human oligomeric Aβ1-42, which recapitulate the Aβ burden reported in human donor eyes. In vitro studies investigated the cellular effects of Aβ in endothelial and retinal pigment epithelial (RPE) cells. Results show subretinal Aβ-induced focal AMD-like pathology within 2 weeks. Aβ exposure caused endothelial cell migration, and morphological and barrier alterations to the RPE. Aβ co-localized to late-endocytic compartments of RPE cells, which persisted despite attempts to clear it through upregulation of lysosomal cathepsin B, revealing a novel mechanism of lysosomal impairment in retinal degeneration. The rapid upregulation of cathepsin B was out of step with the prolonged accumulation of Aβ within lysosomes, and contrasted with enzymatic responses to internalized photoreceptor outer segments (POS). Furthermore, RPE cells exposed to Aβ were identified as deficient in cargo-carrying lysosomes at time points that are critical to POS degradation. These findings imply that Aβ accumulation within late-endocytic compartments, as well as lysosomal deficiency, impairs RPE function over time, contributing to visual defects seen in aging and AMD eyes.
The Value of Non-Referential Gestures: A Systematic Review of Their Cognitive and Linguistic Effects in Children’s Language Development
Authors: Vilà-Giménez Ingrid , Prieto Pilar
Published: February 2021

Speakers produce both referential gestures, which depict properties of a referent, and non-referential gestures, which lack semantic content. While a large number of studies have demonstrated the cognitive and linguistic benefits of referential gestures as well as their precursor and predictive role in both typically developing (TD) and non-TD children, less is known about non-referential gestures in cognitive and complex linguistic domains, such as narrative development. This paper is a systematic review and narrative synthesis of the research concerned with assessing the effects of non-referential gestures in such domains. A search of the literature turned up 11 studies, collectively involving 898 2- to 8-year-old TD children. Although they yielded contradictory evidence, pointing to the need for further investigations, the results of the six studies–in which experimental tasks and materials were pragmatically based–revealed that non-referential gestures not only enhance information recall and narrative comprehension but also act as predictors and causal mechanisms for narrative performance. This suggests that their bootstrapping role in language development is due to the fact that they have important discourse–pragmatic functions that help frame discourse. These findings should be of particular interest to teachers and future studies could extend their impact to non-TD children.
Deep Learning for Fake News Detection in a Pairwise Textual Input Schema
Authors: Mouratidis Despoina , Nikiforos Maria Nefeli , Kermanidis Katia Lida
Published: February 2021

In the past decade, the rapid spread of large volumes of online information among an increasing number of social network users is observed. It is a phenomenon that has often been exploited by malicious users and entities, which forge, distribute, and reproduce fake news and propaganda. In this paper, we present a novel approach to the automatic detection of fake news on Twitter that involves (a) pairwise text input, (b) a novel deep neural network learning architecture that allows for flexible input fusion at various network layers, and (c) various input modes, like word embeddings and both linguistic and network account features. Furthermore, tweets are innovatively separated into news headers and news text, and an extensive experimental setup performs classification tests using both. Our main results show high overall accuracy performance in fake news detection. The proposed deep learning architecture outperforms the state-of-the-art classifiers, while using fewer features and embeddings from the tweet text.
Clinical Application of Liver Imaging Reporting and Data System for Characterizing Liver Neoplasms: A Meta-Analysis
Authors: Li Lingling , Hu Yixin , Han Jing , Li Qing , Peng Chuan , Zhou Jianhua
Published: February 2021

The Liver Imaging Reporting and Data System (LI-RADS) is a comprehensive system for standardizing liver imaging in patients at risk of developing hepatocellular carcinoma (HCC). We aimed to determine the diagnostic performance of LI-RADS category 5 (LR5) for diagnosing HCC and LI-RADS category M (LRM) for characterizing other non-HCC malignancies (OM) using contrast-enhanced ultrasound (CEUS) and computed tomography (CT)/magnetic resonance imaging (MRI). Multiple databases were searched for articles evaluating the diagnostic accuracy of CEUS LI-RADS and/or CT/MRI LI-RADS. A random-effects model was adopted to synthesize the summary estimates of the diagnostic accuracy of LR5 for diagnosing HCC and LRM for characterizing OM using CEUS and CT/MRI. The pooled sensitivity and specificity of CEUS LR5 for the diagnosis of HCC were 69% and 93%, respectively. The pooled sensitivity was 67% and the specificity, 93% of CT/MRI LR5 for HCC diagnosis. There was no significant difference between the overall diagnostic accuracy for HCC diagnosis of CEUS LR5 and that of CT/MRI LR5 in terms of diagnostic odds ratio (DOR) (p = 0.55). The sensitivity was 84% with a specificity of 90% in the CEUS LRM for characterizing OM, while the sensitivity and specificity of CT/MRI LRM for characterizing OM was 63% and 95%. The DOR of CEUS LRM for characterizing OM was higher than that of CT/MRI LRM without significant difference (50.59 vs. 36.06, p = 0.34). This meta-analysis indicated that CEUS LI-RADS is qualified to characterize HCC and OM and may provide complementary information on liver nodules to CT/MRI LI-RADS.
The Cardiac Effects of Performance-Enhancing Medications: Caffeine vs. Anabolic Androgenic Steroids
Authors: Sivalokanathan Sanjay , Małek Łukasz A. , Malhotra Aneil
Published: February 2021

Several performance-enhancing or ergogenic drugs have been linked to both significant adverse cardiovascular effects and increased cardiovascular risk. Even with increased scrutiny on the governance of performance-enhancing drugs (PEDs) in professional sport and heightened awareness of the associated cardiovascular risk, there are some who are prepared to risk their use to gain competitive advantage. Caffeine is the most commonly consumed drug in the world and its ergogenic properties have been reported for decades. Thus, the removal of caffeine from the World Anti-Doping Agency (WADA) list of banned substances, in 2004, has naturally led to an exponential rise in its use amongst athletes. The response to caffeine is complex and influenced by both genetic and environmental factors. Whilst the evidence may be equivocal, the ability of an athlete to train longer or at a greater power output cannot be overlooked. Furthermore, its impact on the myocardium remains unanswered. In contrast, anabolic androgenic steroids are recognised PEDs that improve athletic performance, increase muscle growth and suppress fatigue. Their use, however, comes at a cost, afflicting the individual with several side effects, including those that are detrimental to the cardiovascular system. This review addresses the effects of the two commonest PEDs, one legal, the other prohibited, and their respective effects on the heart, as well as the challenge in defining its long-term implications.
A Quickly Deployed and UAS-Based Logistics Network for Delivery of Critical Medical Goods during Healthcare System Stress Periods: A Real Use Case in Valencia (Spain)
Authors: Quintanilla García Israel , Vera Vélez Norberto , Alcaraz Martínez Pablo , Vidal Ull Jordi , Fernández Gallo Beatriz
Published: February 2021

On the one hand, Unmanned Aircraft Systems (UASs) have experienced great applicability surge in the recent years, arising as a promising technology with a wide field of use. On the other hand, healthcare, a critical system in modern society, is subject to a heavy and unexpected pressure in the case of situations such as the COVID-19 pandemic. This article aims to leverage the flexibility of UASs as complementary support for healthcare logistic systems when under high-stress conditions, via quick deployment of an air delivery network. We have defined a logistics network model and created three scenarios based on the model and current needs in Valencia (Spain). Flight tests have been performed in these scenarios, which include urban areas and controlled airspace. Operations complied with requirements derived from the application of Specific Operations Risk Assessment (SORA) methodology, recently adopted by the European Aviation Safety Agency (EASA). Flights were successful, being able to swiftly deliver medical goods without requiring any dedicated infrastructure. However, a moderate number of contingencies took place during the tests, mainly related to control link quality and Air Traffic Management (ATM) integration, forcing the use of dedicated procedures to cope with them. Although additional development is required to ensure the safety of large-scale automated operations, the use of UASs as part of logistic networks is a feasible means to support existing structures, especially in situations in dire need.
Time-Periodic Cooling of Rayleigh–Bénard Convection
Authors: Nasseri Lyes , Himrane Nabil , Ameziani Djamel Eddine , Bourada Abderrahmane , Bennacer Rachid
Published: February 2021

The problem of Rayleigh–Bénard’s natural convection subjected to a temporally periodic cooling condition is solved numerically by the Lattice Boltzmann method with multiple relaxation time (LBM-MRT). The study finds its interest in the field of thermal comfort where current knowledge has gaps in the fundamental phenomena requiring their exploration. The Boussinesq approximation is considered in the resolution of the physical problem studied for a Rayleigh number taken in the range 103 ≤ Ra ≤ 106 with a Prandtl number equal to 0.71 (air as working fluid). The physical phenomenon is also controlled by the amplitude of periodic cooling where, for small values of the latter, the results obtained follow a periodic evolution around an average corresponding to the formulation at a constant cold temperature. When the heating amplitude increases, the physical phenomenon is disturbed, the stream functions become mainly multicellular and an aperiodic evolution is obtained for the heat transfer illustrated by the average Nusselt number.
Influence of Age and Individual Differences on Mouthfeel Perception of Whey Protein-Fortified Products: A Review
Authors: Norton Victoria , Lignou Stella , Methven Lisa
Published: February 2021

Protein needs are considered to increase with age, with protein consumption being associated with many positive outcomes. Protein-fortified products are often used to improve nutritional status and prevent age-related muscle mass loss in older adults. Accordingly, older adults are commonly provided with products fortified with whey protein; however, such products can cause mouthdrying, limiting consumption and product enjoyment. Currently, the extent to which age and individual differences (e.g., saliva, oral health, food oral processing) influence the perception of whey protein-derived mouthdrying is relatively unclear. Previous research in this area has mainly focused on investigating mouthdrying, without taking into account individual differences that could influence this perception within the target population. Therefore, the main focus of this review is to provide an overview of the relevant individual differences likely to influence mouthfeel perception (specifically mouthdrying) from whey protein-fortified products, thereby enabling the future design of such products to incorporate better the needs of older adults and improve their nutritional status. This review concludes that age and individual differences are likely to influence mouthdrying sensations from whey protein-fortified products. Future research should focus more on the target population and individual differences to maximise the benefits from whey protein fortification.
Luminescent Behavior of Gels and Sols Comprised of Molecular Gelators
Authors: Grover Girishma , Weiss Richard G.
Published: February 2021

We present a brief review of some important conceptual and practical aspects for the design and properties of molecular luminescent gelators and their gels. Topics considered include structural and dynamic aspects of the gels, including factors important to their ability to emit radiation from electronically excited states.
Prevalent ALMS1 Pathogenic Variants in Spanish Alström Patients
Authors: Bea-Mascato Brais , Solarat Carlos , Perea-Romero Irene , Jaijo Teresa , Blanco-Kelly Fiona , Millán José M. , Ayuso Carmen , Valverde Diana
Published: February 2021

Alström syndrome (ALMS) is an ultrarare disease with an estimated prevalence lower than 1 in 1,000,000. It is associated with disease-causing mutations in the Alström syndrome 1 (ALMS1) gene, which codifies for a structural protein of the basal body and centrosomes. The symptomatology involves nystagmus, type 2 diabetes mellitus (T2D), obesity, dilated cardiomyopathy (DCM), neurodegenerative disorders and multiorgan fibrosis. We refined the clinical and genetic diagnosis data of 12 patients from 11 families, all of them from Spain. We also studied the allelic frequency of the different variants present in this cohort and performed a haplotype analysis for the most prevalent allele. The genetic analysis revealed 2 novel homozygous variants located in the exon 8, p.(Glu929Ter) and p.(His1808GlufsTer20) in 2 unrelated patients. These 2 novel variants were classified as pathogenic after an in silico experiment (computer analysis). On the other hand, 2 alleles were detected at a high frequency in our cohort: p.(Tyr1714Ter) (25%) and p.(Ser3872TyrfsTer19) (16.7%). The segregation analysis showed that the pathogenic variant p.(Tyr1714Ter) in 3 families is linked to a rare missense polymorphism, p.(Asn1787Asp). In conclusion, 2 novel pathological mutations have been discovered in homozygosis, as well as a probable founder effect in 3 unrelated families.
The Influence of Maturity Status on Resting Energy Expenditure, Body Composition and Blood Pressure in Physically Active Children
Authors: Łuszczki Edyta , Kuchciak Maciej , Dereń Katarzyna , Bartosiewicz Anna
Published: February 2021

Peak height velocity (PHV) is the period where the maximum rate of growth occurs. The moment the sports player reaches PHV can be estimated by monitoring the growth of body structures. The aim of this study was to assess changes in resting energy expenditure (REE), body composition and blood pressure in young, male soccer players between the pre-PHV, circa-PHV and post-PHV periods. This transverse study was conducted among 184 children aged 9 to 17 and included measurements of the resting energy expenditure (REE) using indirect calorimetry, body composition (bioimpedance) and blood pressure (sphygmomanometer). In addition, births in each quartile were analyzed. Children in the pre-PHV group had significantly lower REE values compared to the other two groups (p < 0.0001). The differences in the value of the REE between the children in the circum and post groups were not statistically significant. Additionally, the fat-free mass was significantly lower in the pre-PHV period compared to the other two periods (p < 0.0001), and the same relationship concerned the z-score body mass index (BMI) and systolic blood pressure. Early-born players were overrepresented (p < 0.05).
Combinatorial K-Means Clustering as a Machine Learning Tool Applied to Diabetes Mellitus Type 2
Authors: Nedyalkova Miroslava , Madurga Sergio , Simeonov Vasil
Published: February 2021

A new original procedure based on k-means clustering is designed to find the most appropriate clinical variables able to efficiently separate into groups similar patients diagnosed with diabetes mellitus type 2 (DMT2) and underlying diseases (arterial hypertonia (AH), ischemic heart disease (CHD), diabetic polyneuropathy (DPNP), and diabetic microangiopathy (DMA)). Clustering is a machine learning tool for discovering structures in datasets. Clustering has been proven to be efficient for pattern recognition based on clinical records. The considered combinatorial k-means procedure explores all possible k-means clustering with a determined number of descriptors and groups. The predetermined conditions for the partitioning were as follows: every single group of patients included patients with DMT2 and one of the underlying diseases; each subgroup formed in such a way was subject to partitioning into three patterns (good health status, medium health status, and degenerated health status); optimal descriptors for each disease and groups. The selection of the best clustering is obtained through the parameter called global variance, defined as the sum of all variance values of all clinical variables of all the clusters. The best clinical parameters are found by minimizing this global variance. This methodology has to identify a set of variables that are assumed to separate each underlying disease efficiently in three different subgroups of patients. The hierarchical clustering obtained for these four underlying diseases could be used to build groups of patients with correlated clinical data. The proposed methodology gives surmised results from complex data based on a relationship with the health status of the group and draws a picture of the prediction rate of the ongoing health status.
Analysis of the Impact of Human–Cobot Collaborative Manufacturing Implementation on the Occupational Health and Safety and the Quality Requirements
Authors: Pauliková Alena , Gyurák Babeľová Zdenka , Ubárová Monika
Published: February 2021

Implementing Industry 4.0 and interconnected robotization in industrial enterprises drifts towards occupational changes. Nowadays, the task is to create cooperation and collaboration between a robot and a human in a common robotized workplace so that it is safe and effective. The type of robot, the robotic device that works in collaboration with a human operator, is called a cobot. In the case of a closer interaction of the robot or cobot with humans, it is necessary to consider where it is possible to replace human work entirely or where it is possible to merely supplement it. The most socially acceptable option is the implementation of robots only for the performance of supplementary tasks, since the traditional work positions of people in manufacturing processes would remain largely preserved. On the other hand, workplace robotization is particularly suitable for work environments with hazardous chemical substances that are carcinogenic and toxic to humans. Similarly, robotization helps to improve workplace ergonomics and also to avoid, for humans, very laborious and often repetitive work. The SWOT analysis (analysis of Strengths, Weaknesses, Opportunities, and Threats) was used as a relevant tool to assess various aspects of the impact of increasing robotization on working positions in industrial enterprises. SWOT analysis is an indicative assessment of the suitability of implementation of robots in a given workplace, which helps to create an optimal solution and indicate new areas of needed analysis and research directions.
Cultural Control of Drosophila suzukii in Small Fruit—Current and Pending Tactics in the U.S.
Authors: Schöneberg Torsten , Lewis Margaret T. , Burrack Hannah J. , Grieshop Matthew , Isaacs Rufus , Rendon Dalila , Rogers Mary , Rothwell Nikki , Sial Ashfaq A. , Walton Vaughn M. , Hamby Kelly A.
Published: February 2021

Spotted-wing drosophila, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), a vinegar fly of Asian origin, has emerged as a devastating pest of small and stone fruits throughout the United States. Tolerance for larvae is extremely low in fresh market fruit, and management is primarily achieved through repeated applications of broad-spectrum insecticides. These applications are neither economically nor environmentally sustainable, and can limit markets due to insecticide residue restrictions, cause outbreaks of secondary pests, and select for insecticide resistance. Sustainable integrated pest management programs include cultural control tactics and various nonchemical approaches for reducing pest populations that may be useful for managing D. suzukii. This review describes the current state of knowledge and implementation for different cultural controls including preventative tactics such as crop selection and exclusion as well as strategies to reduce habitat favorability (pruning; mulching; irrigation), alter resource availability (harvest frequency; sanitation), and lower suitability of fruit postharvest (cooling; irradiation). Because climate, horticultural practices, crop, and market underlie the efficacy, feasibility, and affordability of cultural control tactics, the potential of these tactics for D. suzukii management is discussed across different production systems.
Incisor Occlusion Affects Profile Shape Variation in Middle-Aged Adults
Authors: Kanavakis Georgios , Silvola Anna-Sofia , Halazonetis Demetrios , Lähdesmäki Raija , Pirttiniemi Pertti
Published: February 2021

Background: The aim of this study was to assess the effect of overjet and overbite on profile shape in middle–aged individuals. Methods: The study population comprised 1754 46-year-old individuals, members of the 1966 Northern Finland Birth Cohort. Their profile images were digitized using 48 landmarks and semi-landmarks. The subsequent landmark coordinates were then transformed to shape coordinates through Procrustes Superimposition, and final data were reduced into Principal Components (PCs) of shape. Overjet and overbite values were measured manually, during a clinical examination. A multivariate regression model was developed to evaluate the effect of overjet and overbite on profile shape. Results: The first nine PCs described more than 90% of profile shape variation in the sample and were used as the shape variables in all subsequent analyses. Overjet predicted 21.3% of profile shape in the entire sample (η2overjet = 0.213; p < 0.001), while the effect of overbite was weaker (η2overbite = 0.138; p < 0.001). In males, the equivalent effects were 22.6% for overjet and 14% for overbite, and in females, 25.5% and 13.5%, respectively. Conclusion: Incisor occlusion has a noteworthy effect on profile shape in middle-aged adults. Its impact becomes more significant taking into consideration the large variety of genetic and environmental factors affecting soft tissue profile.