bluuebunny/crossref_metadata_2025 huggingface dataset:
USING SEDIMENTOLOGICAL PRIORS TO IMPROVE 14C CALIBRATION OF BIOTURBATED SEDIMENT ARCHIVES
Authors: Lougheed Bryan C
Published: January 2022

Radiocarbon (14C) dating is often carried out upon multi-specimen samples sourced from bioturbated sediment archives, such as deep-sea sediment. These samples are inherently heterogeneous in age, but existing 14C calibration techniques were originally developed for age homogeneous material, such as archaeological artifacts or individual tree rings. A lack of information about age heterogeneity leads to a systematic underestimation of a sample’s true age range, as well as the possible generation of significant age-depth artifacts during periods of the Earth’s history coinciding with highly dynamic atmospheric Δ14C. Here, a new calibration protocol is described that allows for the application of sedimentological priors describing sediment accumulation rate, bioturbation depth and temporally dynamic species abundance. This Bayesian approach produces a credible calibrated age distribution associated with a particular laboratory 14C determination and its associated sedimentological priors, resulting in an improved calibration, especially in the case of low sediment accumulation rates typical of deep-sea sediment. A time-optimized computer script (biocal) for the new calibration protocol is also presented, thus allowing for rapid and automated application of the new calibration protocol. This new calibration protocol could be applied within existing age-depth modeling software packages to produce more accurate geochronologies for bioturbated sediment archives.
Hobbes's Eschatology and Scriptural Interpretation in Leviathan
Authors: OKADA TAKUYA
Published: January 2022

Hobbes's eschatology in Leviathan is one of the most striking aspects of this classic work and has received considerable scholarly attention. Nevertheless, its scriptural interpretation has rarely been examined. This article closely analyses Hobbes's scriptural case for two aspects of eschatology: the doctrine of mortalism and the terrestrial kingdom of God. It shows that, to a large extent, Hobbes's biblical exegesis for these two eschatological issues was preceded by that of his contemporaries, including Richard Overton and John Archer. It is likely, in particular, that the scriptural interpretation for Hobbes's mortalism was directly indebted to Overton's Mans mortalitie.
Agrarian measures in the kingdom of Granada before and after the Castilian conquest: the lands of the Alpujarra
Authors: Carballeira Debasa Ana María
Published: January 2022

The aim of this article is to broaden our knowledge of the metrological system of the Morisco period in the kingdom of Granada and, more specifically, in the rural area of the Alpujarra. The sources used in the analysis are two inventories of ecclesiastical goods from Islamic origin, drawn up in 1527 and 1530, four decades after the culmination of the Christian conquest of al-Andalus. The article explores the agrarian units of measurement used by the Morisco community of the Alpujarra in their daily lives (measures of surface area and length, weight or mass, volume or capacity, and of water used for irrigation). As far as possible, equivalences are presented for the units discussed. Attempts are made to establish whether these measures were inherited from the Andalusi period or whether, on the contrary, they were the result of the implementation of Castilian legislation on the matter. The data gathered have revealed the existence of a hybrid measurement system, which shows that the ordinances to modify the system of weights and measures of the Muslims and to seek a correspondence with the Castilian system had little effect in the Alpujarra, where, in many cases, the Islamic system of measurement continued in use. This circumstance indicates that the changes and transformations in the daily life of this rural community after the Christian conquest were not as imminent as might have been expected.
Necrotic enteritis in chickens: a review of pathogenesis, immune responses and prevention, focusing on probiotics and vaccination
Authors: Alizadeh Mohammadali , Shojadoost Bahram , Boodhoo Nitish , Astill Jake , Taha-Abdelaziz Khaled , Hodgins Douglas C. , Kulkarni Raveendra R. , Sharif Shayan
Published: January 2022

Necrotic enteritis (NE), caused by Clostridium perfringens (CP), is one of the most common of poultry diseases, causing huge economic losses to the poultry industry. This review provides an overview of the pathogenesis of NE in chickens and of the interaction of CP with the host immune system. The roles of management, nutrition, probiotics, and vaccination in reducing the incidence and severity of NE in poultry flocks are also discussed.
Three Patterns of Inheritance of Quantitative Dermatoglyphic Traits: Kosovo Albanian Twin Study
Authors: Temaj Gazmend , Škarić-Jurić Tatjana , Butković Ana , Behluli Emir , Zajc Petranović Matea , Moder Angelika
Published: January 2022

Dermatoglyphs are epidermal ridge configurations on the fingers, palms and soles that are formed during fetal development, and therefore only the intrauterine environment can have any influence on their formation. This study aims at investigating the genetic and environmental contribution in determining quantitative dermatoglyphic traits in 32 monozygotic (MZ) and 35 dizygotic (DZ) same-sex twins from the Albanian population of Kosovo. All genetic analyses were run in the statistical program Mx. After assumptions testing, based on the pattern of MZ–DZ correlations, univariate models were fitted to the data in order to estimate additive genetic (A), common (C) and individual (E) environmental influences for all variables. The exception was the atd-angle for which a model with nonadditive genetic (D) influences was tested, since DZ correlations were less than half of MZ correlations. Goodness of fit of the full ACE or ADE model was compared to the saturated model. The fit of nested models (AE, CE, DE or E) was compared to the full models (ACE or ADE). Our results indicate that additive genetic component strongly contributes to individual differences in finger ridge counts (49−81%), and weakly (0−50%) on the formation of the palmar ridge counts between the palmar triradii a, b, c, and d. The specific pattern found for the atd-angle implies the impact of a nonadditive genetic component, possibly the effect of a major gene. Further, more powered studies are needed to confirm this pattern, especially for resolving the issue of the huge difference in MZ and DZ twin similarity for the atd-angle palmar trait.
A Regional SAR Dark Spot Detection Method Based on GMTRJ Algorithm
Authors: Li M. M. , Wang Y. , Liu J. D. , Zhang Y. F. , Liang T. Y.
Published: January 2022

Generalized Multiple‐Try Reversible Jump (GMTRJ) is a recent algorithm which makes it possible to improve the accurate tuning of the jump proposals in the Reversible Jump Markov Chain Monte Carlo algorithm. In this paper, the GMTRJ algorithm is combined the regular tessellation and the Bayesian paradigm to propose a regional synthetic aperture radar (SAR) dark spot detection method. First, the regular tessellation and the Bayesian paradigm are applied to build a regional SAR dark spot detection model; then the GMTRJ algorithm is designed to simulate from the detection model to obtain the optimal regional detection result. As the blocks may cross the boundaries between dark spot and ocean background regions, a refined operation is designed to improve the detection accuracy of boundaries. We present test results demonstrating the feasibility and advantages of the GMTRJ algorithm on simulating from the detection model, and illustrating the feasibility of the refined SAR dark spot detection operation on improving the detection accuracy of boundaries.
Satellite Laser Altimetry Reveals a Net Water Mass Gain in Global Lakes With Spatial Heterogeneity in the Early 21st Century
Authors: Luo Shuangxiao , Song Chunqiao , Ke Linghong , Zhan Pengfei , Fan Chenyu , Liu Kai , Chen Tan , Wang Jida , Zhu Jingying
Published: January 2022

Lake water storage changes are important factors that influence the climate, hydrological cycle, and environments. However, long‐term estimation of global lake storage changes is challenging because historical in‐situ hydrological observations worldwide are rarely available. Benefiting from the laser altimeter ICESat and ICESat‐2, we comprehensively assessed water level and volume changes in global natural lakes larger than 10 km2 during 2003–2020. The 6,567 lakes observable by ICESat/ICESat‐2, which account for ∼94% of the total global lake volume, showed a total water storage increase of 10.88 ± 16.45 Gt/yr during 2003–2020, and the estimate reaches 16.12 ± 20.41 Gt/yr when also taking account of the remaining unobserved lakes. Despite water gains in most natural lakes, large lakes under dry and high water‐stress conditions experienced dramatic water loss in general. Presumably, these drying lakes may continue to shrink with a warming climate and continuously increasing water demands in the future without further action.
Different Sporadic‐E (Es) Layer Types Development During the August 2018 Geomagnetic Storm: Evidence of Auroral Type (Esa) Over the SAMA Region
Authors: Moro J. , Xu J. , Denardini C. M. , Resende L. C. A. , Da Silva L. A. , Chen S. S. , Carrasco A. J. , Liu Z. , Wang C. , Schuch N. J.
Published: January 2022

Sporadic‐E (Es) layers are investigated over five Digisonde stations located inside (Santa Maria and Cachoeira Paulista), boundary (São Luís), and outside (Millstone Hill and Port Stanley) the South American Magnetic Anomaly (SAMA) in the course of the 25 August 2018, geomagnetic storm. Data from Digisondes and ground‐based magnetometer and a numerical model of the E‐region (MIRE) are analyzed to search the roles of neutral winds and electric fields as physical processes responsible for the generation of Es layers. Moreover, it is observed for the first time over Santa Maria a type of Es layer that resembles the Esa detected in the auroral region. The characterization of such signatures in ionograms and the investigations of its formation mechanism are the main focus of this study. Hiss waves activities detected by the Van Allen Probe‐B data provide evidence that energetic particle precipitations are the most likely process responsible for this type of Es layer inside the SAMA in the night hours during the geomagnetic storm recovery phase. An attempt is also made to explain the presence of Esq over São Luís in terms of the expansion of the Equatorial Electrojet to higher latitudes. Finally, the results bring a new observational and modeling indication of a concomitant action of multiple formation mechanisms in the Es layers development in the American sector during the 25 August 2018, geomagnetic storm.
A Bayesian Fuzzy Clustering Approach for Design of Precipitation Gauge Network Using Merged Remote Sensing and Ground‐Based Precipitation Products
Authors: Sreeparvathy Vijay , Srinivas V. V.
Published: January 2022

A two‐level clustering approach is proposed for optimal design/expansion of a ground‐based precipitation monitoring network (GPN). It harnesses the advantages of Infinite Bayesian fuzzy clustering in the first level to partition the study area into homogeneous precipitation zones by considering structural/statistical characteristics and temporal variability of the observed precipitation. In the second level, an ensemble of hierarchical and partitional clustering techniques is considered in the time domain to effectively partition each zone into groups by considering weighted inter‐site dissimilarities of precipitation. The dissimilarities account for correlation, temporal dynamics, and fuzzy mutual information of precipitation at existing stations and possible new gauge locations. Key station’s location in each group is identified by a proposed ranking procedure that accounts for population density, land‐use/landcover, and fuzzy marginal entropy of precipitation. For use with the approach, information on precipitation was derived for fine resolution ungauged grids covering the study area using random forest‐based regression relationships developed for gauged grids between merged multiple satellite‐based precipitation products (CHIRPS, IMERG) and ground‐based precipitation measurements. The potential of the proposed approach over other clustering‐based procedures is illustrated through a case study on a GPN comprising 1,128 gauges in Karnataka state (191,791 km2) of India. Potential locations for installing new gauges and areas where there is scope for relocating existing stations are identified. The proposed methodology appears promising and could be extended to design networks monitoring various other hydrometeorological variables.
Science Off the Seashore
Authors: Goss Heather
Published: January 2022

In our February issue, Eos reports on the study of the ocean and our relationship to it, in the spirit of the United Nations Decade of Ocean Science for Sustainable Development.
A New Mayflower, Named for the Past, Autonomously Navigates the Future
Authors: Tripathy-Lang Alka
Published: January 2022

To commemorate the 400th anniversary of the Pilgrims’ crossing, a ship guided by an AI captain will embark on the same journey, doing science along the way.
Tracing PRX1+ cells during molar formation and periodontal ligament reconstruction
Authors: Gong Xuyan , Zhang Han , Xu Xiaoqiao , Ding Yunpeng , Yang Xingbo , Cheng Zhiyang , Tao Dike , Hu Congjiao , Xiang Yaozu , Sun Yao
Published: January 2022

Neural crest-derived mesenchymal stem cells (MSCs) are known to play an essential function during tooth and skeletal development. PRX1+ cells constitute an important MSC subtype that is implicated in osteogenesis. However, their potential function in tooth development and regeneration remains elusive. In the present study, we first assessed the cell fate of PRX1+ cells during molar development and periodontal ligament (PDL) formation in mice. Furthermore, single-cell RNA sequencing analysis was performed to study the distribution of PRX1+ cells in PDL cells. The behavior of PRX1+ cells during PDL reconstruction was investigated using an allogeneic transplanted tooth model. Although PRX1+ cells are spatial specific and can differentiate into almost all types of mesenchymal cells in first molars, their distribution in third molars is highly limited. The PDL formation is associated with a high number of PRX1+ cells; during transplanted teeth PDL reconstruction, PRX1+ cells from the recipient alveolar bone participate in angiogenesis as pericytes. Overall, PRX1+ cells are a key subtype of dental MSCs involved in the formation of mouse molar and PDL and participate in angiogenesis as pericytes during PDL reconstruction after tooth transplantation.
PDGFRα reporter activity identifies periosteal progenitor cells critical for bone formation and fracture repair
Authors: Xu Jiajia , Wang Yiyun , Li Zhu , Tian Ye , Li Zhao , Lu Amy , Hsu Ching-Yun , Negri Stefano , Tang Cammy , Tower Robert J. , Morris Carol , James Aaron W.
Published: January 2022

The outer coverings of the skeleton, which is also known as the periosteum, are arranged in concentric layers and act as a reservoir for tissue-specific bone progenitors. The cellular heterogeneity within this tissue depot is being increasingly recognized. Here, inducible PDGFRα reporter animals were found to mark a population of cells within the periosteum that act as a stem cell reservoir for periosteal appositional bone formation and fracture repair. During these processes, PDGFRα reporter+ progenitors give rise to Nestin+ periosteal cells before becoming osteoblasts and osteocytes. The diphtheria toxin-mediated ablation of PDGFRα reporter+ cells led to deficits in cortical bone formation during homeostasis and a diminutive hard callus during fracture repair. After ossicle transplantation, both mouse PDGFRα reporter+ periosteal cells and human Pdgfrα+ periosteal progenitors expand, ossify, and recruit marrow to a greater extent than their counterpart periosteal cells, whereas PDGFRα reporter− periosteal cells exhibit a predisposition to chondrogenesis in vitro. Total RNA sequencing identified enrichment of the secreted factors Fermt3 and Ptpn6 within PDGFRα reporter+ periosteal cells, which partly underlie the osteoblastogenic features of this cell population.
High-resolution structures of a thermophilic eukaryotic 80S ribosome reveal atomistic details of translocation
Authors: Kišonaitė Miglė , Wild Klemens , Lapouge Karine , Ruppert Thomas , Sinning Irmgard
Published: January 2022

Ribosomes are complex and highly conserved ribonucleoprotein assemblies catalyzing protein biosynthesis in every organism. Here we present high-resolution cryo-EM structures of the 80S ribosome from a thermophilic fungus in two rotational states, which due to increased 80S stability provide a number of mechanistic details of eukaryotic translation. We identify a universally conserved ‘nested base-triple knot’ in the 26S rRNA at the polypeptide tunnel exit with a bulged-out nucleotide that likely serves as an adaptable element for nascent chain containment and handover. We visualize the structure and dynamics of the ribosome protective factor Stm1 upon ribosomal 40S head swiveling. We describe the structural impact of a unique and essential m1acp3 Ψ 18S rRNA hyper-modification embracing the anticodon wobble-position for eukaryotic tRNA and mRNA translocation. We complete the eEF2-GTPase switch cycle describing the GDP-bound post-hydrolysis state. Taken together, our data and their integration into the structural landscape of 80S ribosomes furthers our understanding of protein biogenesis.
Symmetries in quantum networks lead to no-go theorems for entanglement distribution and to verification techniques
Authors: Hansenne Kiara , Xu Zhen-Peng , Kraft Tristan , Gühne Otfried
Published: January 2022

Quantum networks are promising tools for the implementation of long-range quantum communication. The characterization of quantum correlations in networks and their usefulness for information processing is therefore central for the progress of the field, but so far only results for small basic network structures or pure quantum states are known. Here we show that symmetries provide a versatile tool for the analysis of correlations in quantum networks. We provide an analytical approach to characterize correlations in large network structures with arbitrary topologies. As examples, we show that entangled quantum states with a bosonic or fermionic symmetry can not be generated in networks; moreover, cluster and graph states are not accessible. Our methods can be used to design certification methods for the functionality of specific links in a network and have implications for the design of future network structures.
A self-supervised domain-general learning framework for human ventral stream representation
Authors: Konkle Talia , Alvarez George A.
Published: January 2022

Anterior regions of the ventral visual stream encode substantial information about object categories. Are top-down category-level forces critical for arriving at this representation, or can this representation be formed purely through domain-general learning of natural image structure? Here we present a fully self-supervised model which learns to represent individual images, rather than categories, such that views of the same image are embedded nearby in a low-dimensional feature space, distinctly from other recently encountered views. We find that category information implicitly emerges in the local similarity structure of this feature space. Further, these models learn hierarchical features which capture the structure of brain responses across the human ventral visual stream, on par with category-supervised models. These results provide computational support for a domain-general framework guiding the formation of visual representation, where the proximate goal is not explicitly about category information, but is instead to learn unique, compressed descriptions of the visual world.
Pickering emulsion droplet-based biomimetic microreactors for continuous flow cascade reactions
Authors: Zhang Ming , Ettelaie Rammile , Dong Lianlian , Li Xiaolong , Li Ting , Zhang Xiaoming , Binks Bernard P. , Yang Hengquan
Published: January 2022

A continuous flow cascade of multi-step catalytic reactions is a cutting-edge concept to revolutionize stepwise catalytic synthesis yet is still challenging in practical applications. Herein, a method for practical one-pot cascade catalysis is developed by combining Pickering emulsions with continuous flow. Our method involves co-localization of different catalytically active sub-compartments within droplets of a Pickering emulsion yielding cell-like microreactors, which can be packed in a column reactor for continuous flow cascade catalysis. As exemplified by two chemo-enzymatic cascade reactions for the synthesis of chiral cyanohydrins and chiral ester, 5 − 420 fold enhancement in the catalysis efficiency and as high as 99% enantioselectivity were obtained even over a period of 80 − 240 h. The compartmentalization effect and enriching-reactant properties arising from the biomimetic microreactor are theoretically and experimentally identified as the key factors for boosting the catalysis efficiency and for regulating the kinetics of cascade catalysis.
Rashba splitting in organic–inorganic lead–halide perovskites revealed through two-photon absorption spectroscopy
Authors: Lafalce Evan , Amerling Eric , Yu Zhi-Gang , Sercel Peter C. , Whittaker-Brooks Luisa , Vardeny Z. Valy
Published: January 2022

The Rashba splitting in hybrid organic–inorganic lead–halide perovskites (HOIP) is particularly promising and yet controversial, due to questions surrounding the presence or absence of inversion symmetry. Here we utilize two-photon absorption spectroscopy to study inversion symmetry breaking in different phases of these materials. This is an all-optical technique to observe and quantify the Rashba effect as it probes the bulk of the materials. In particular, we measure two-photon excitation spectra of the photoluminescence in 2D, 3D, and anionic mixed HOIP crystals, and show that an additional band above, but close to the optical gap is the signature of new two-photon transition channels that originate from the Rashba splitting. The inversion symmetry breaking is believed to arise from ionic impurities that induce local electric fields. The observation of the Rashba splitting in the bulk of HOIP has significant implications for the understanding of their spintronic and optoelectronic device properties.
Nodal is a short-range morphogen with activity that spreads through a relay mechanism in human gastruloids
Authors: Liu Lizhong , Nemashkalo Anastasiia , Rezende Luisa , Jung Ji Yoon , Chhabra Sapna , Guerra M. Cecilia , Heemskerk Idse , Warmflash Aryeh
Published: January 2022

Morphogens are signaling molecules that convey positional information and dictate cell fates during development. Although ectopic expression in model organisms suggests that morphogen gradients form through diffusion, little is known about how morphogen gradients are created and interpreted during mammalian embryogenesis due to the combined difficulties of measuring endogenous morphogen levels and observing development in utero. Here we take advantage of a human gastruloid model to visualize endogenous Nodal protein in living cells, during specification of germ layers. We show that Nodal is extremely short range so that Nodal protein is limited to the immediate neighborhood of source cells. Nodal activity spreads through a relay mechanism in which Nodal production induces neighboring cells to transcribe Nodal. We further show that the Nodal inhibitor Lefty, while biochemically capable of long-range diffusion, also acts locally to control the timing of Nodal spread and therefore of mesoderm differentiation during patterning. Our study establishes a paradigm for tissue patterning by an activator-inhibitor pair.
Nitrogen nutrition contributes to plant fertility by affecting meiosis initiation
Authors: Yang Han , Li Yafei , Cao Yiwei , Shi Wenqing , Xie En , Mu Na , Du Guijie , Shen Yi , Tang Ding , Cheng Zhukuan
Published: January 2022

Nitrogen (N), one of the most important plant nutrients, plays crucial roles in multiple plant developmental processes. Spikelets are the primary sink tissues during reproductive growth, and N deficiency can cause floral abortion. However, the roles of N nutrition in meiosis, the crucial step in plant sexual reproduction, are poorly understood. Here, we identified an N-dependent meiotic entrance mutant with loss of function ofELECTRON TRANSFER FLAVOPROTEIN SUBUNIT β(ETFβ) in rice (Oryza sativa).etfβdisplayed meiosis initiation defects, excessive accumulation of branched-chain amino acids (BCAAs) and decrease in total N contents in spikelets under N starvation, which were rescued by applying excess exogenous inorganic N. Under N starvation, ETFβ, through its involvement in BCAA catabolism, promotes N reutilization and contributes to meeting N demands of spikelets, highlighting the impact of N nutrition on meiosis initiation. We conclude that N nutrition contributes to plant fertility by affecting meiosis initiation.
Contemporary mass balance on a cold Eastern Alpine ice cap as a potential link to the Holocene climate
Authors: Fischer Andrea , Stocker-Waldhuber Martin , Frey Martin , Bohleber Pascal
Published: January 2022

Alpine cold ice caps are sensitive indicators of local climate. The adequate interpretation of this information in an ice core requires detailed in situ glaciological and meteorological records, of which there are few. The Weißseespitze summit ice cap (3499 m) presents an ideal case to compare past and present climate and mass balance, with limited ice flow, but close to 6000 years locked into about 10 m of ice. First-ever meteorological observations at the ice dome have revealed that over 3 years of observation most of the accumulation took place between October and December and from April to June. In the colder winter months, between January and March, wind erosion prevents accumulation. Melt occurred between June and September, ice was only affected during short periods, mainly in August, which caused ice losses of up to 0.6 m (i.e. ~ 5% of the total ice thickness). Historical data points at a loss of of 34.9 ± 10.0 m between 1893 and 2018 and almost balanced conditions between 1893 and 1914. The local evidence of ice loss lays the basis for the interpretation of past gaps in the ice core records as past warm/melt events.
Natural quantum reservoir computing for temporal information processing
Authors: Suzuki Yudai , Gao Qi , Pradel Ken C. , Yasuoka Kenji , Yamamoto Naoki
Published: January 2022

Reservoir computing is a temporal information processing system that exploits artificial or physical dissipative dynamics to learn a dynamical system and generate the target time-series. This paper proposes the use of real superconducting quantum computing devices as the reservoir, where the dissipative property is served by the natural noise added to the quantum bits. The performance of this natural quantum reservoir is demonstrated in a benchmark time-series regression problem and a practical problem classifying different objects based on temporal sensor data. In both cases the proposed reservoir computer shows a higher performance than a linear regression or classification model. The results indicate that a noisy quantum device potentially functions as a reservoir computer, and notably, the quantum noise, which is undesirable in the conventional quantum computation, can be used as a rich computation resource.
Road traffic and landscape characteristics predict the occurrence of native halophytes on roadside verges
Authors: Fekete R. , Bak H. , Vincze O. , Süveges K. , Molnár V. A.
Published: January 2022

Road management practices, such as winter de-icing create ideal habitats and competitive advantage for salt-tolerant species. We aimed to map the occurrences of halophytes along roads in Hungary. Furthermore, we tested factors that might play a role in the roadside occurrences of five chosen native halophytes from rare to common, we encountered during our field surveys. These were Festuca pseudovina, Limonium gmelinii subsp. hungaricum, Podospermum canum, Puccinellia distans and Spergularia media. We found, that at least one halophyte species was documented in 71% of the total sampling points. Germination experiments indicated that substrate salt concentration significantly decreased germination rates in each of the five species, but in case of L. gmelinii subsp. hungaricum, or P. distans germination occurred on extremely high salt concentrations. Traffic intensity, the presence of other halophytes at the sampling point and the presence of a given species in the surrounding landscape had a significant positive effect on the occurrence of four of the five model species. Our results suggest that the studied species are mostly in the early stage of their roadside spread, colonizing roadsides close to their native distribution ranges. The possibility of a future range expansion along roads cannot be excluded.
Assessment of fluid unresponsiveness guided by lung ultrasound in abdominal surgery: a prospective cohort study
Authors: Bar Stéphane , Yee Céline , Lichtenstein Daniel , Sellier Magali , Leviel Florent , Abou Arab Osama , Marc Julien , Miclo Matthieu , Dupont Hervé , Lorne Emmanuel
Published: January 2022

A fluid challenge can generate an infraclinical interstitial syndrome that may be detected by the appearance of B-lines by lung ultrasound. Our objective was to evaluate the appearance of B-lines as a diagnostic marker of preload unresponsiveness and postoperative complications in the operating theater. We conducted a prospective, bicentric, observational study. Adult patients undergoing abdominal surgery were included. Stroke volume (SV) was determined before and after a fluid challenge with 250 mL crystalloids (Delta-SV) using esophageal Doppler monitoring. Responders were defined by an increase of Delta-SV > 10% after fluid challenge. B-lines were collected at four bilateral predefined zones (right and left anterior and lateral). Delta-B-line was defined as the number of newly appearing B-lines after a fluid challenge. Postoperative pulmonary complications were prospectively recorded according to European guidelines. In total, 197 patients were analyzed. After a first fluid challenge, 67% of patients were responders and 33% were non-responders. Delta-B-line was significantly higher in non-responders than responders [4 (2–7) vs 1 (0–3), p < 0.0001]. Delta-B-line was able to diagnose fluid non-responders with an area under the curve of 0.74 (95% CI 0.67–0.80, p < 0.0001). The best threshold was two B-lines with a sensitivity of 80% and a specificity of 57%. The final Delta-B-line could predict postoperative pulmonary complications with an area under the curve of 0.74 (95% CI 0.67–0.80, p = 0.0004). Delta-B-line of two or more detected in four lung ultrasound zones can be considered to be a marker of preload unresponsiveness after a fluid challenge in abdominal surgery.

The objectives and procedures of the study were registered at Clinicaltrials.gov (NCT03502460; Principal investigator: Stéphane BAR, date of registration: April 18, 2018).
The impact of FFP3 respirators on the blood saturation
Authors: Wojtasz Izabela , Jaracz Krystyna , Sobczyński Paweł , Drużdż Artur , Dyk Danuta , Kaźmierski Radosław
Published: January 2022

This study aims to investigate whether wearing a filtering facepiece class 3 respirators with personal protective equipment (FPP3/PPE) during work in the intensive care unit (ICU) affects the blood saturation (SpO2), the heart rate (HR), and the well-being of health care workers (HCWs). This preliminary study included a group of 21 volunteers (including 16 females (76%), with a median age of 23 years). Each worker served as his own control and performed the test two times: they wore the FFP3/PPE and did not wear it for a three-hour shift in the ICU. The working with an FFP3/PPE compared to not working with an FFP3/PPE caused a significant, but within normal ranges, influence on the level of SpO2 with a mean decrease of − 1.43%. The highest reduction in the SpO2 was − 2.29% and occurred after 150 min of work. All of the score scales of the well-being markers increased consecutively but moderately during the shift while wearing the FFP3/PPE. We assume that a 3-h shift rhythm is a safe and reliable solution, i.e., three hours of working in the FFP3/PPE in the ICU, followed by rest or working without an FFP3/PPE.
Study on the effect of size on InGaN red micro-LEDs
Authors: Horng Ray-Hua , Ye Chun-Xin , Chen Po-Wei , Iida Daisuke , Ohkawa Kazuhiro , Wu Yuh-Renn , Wuu Dong-Sing
Published: January 2022

In this research, five sizes (100 × 100, 75 × 75, 50 × 50, 25 × 25, 10 × 10 µm2) of InGaN red micro-light emitting diode (LED) dies are produced using laser-based direct writing and maskless technology. It is observed that with increasing injection current, the smaller the size of the micro-LED, the more obvious the blue shift of the emission wavelength. When the injection current is increased from 0.1 to 1 mA, the emission wavelength of the 10 × 10 μm2 micro-LED is shifted from 617.15 to 576.87 nm. The obvious blue shift is attributed to the stress release and high current density injection. Moreover, the output power density is very similar for smaller chip micro-LEDs at the same injection current density. This behavior is different from AlGaInP micro-LEDs. The sidewall defect is more easily repaired by passivation, which is similar to the behavior of blue micro-LEDs. The results indicate that the red InGaN epilayer structure provides an opportunity to realize the full color LEDs fabricated by GaN-based LEDs.
Path planning of scenic spots based on improved A* algorithm
Authors: Wang Xingdong , Zhang Haowei , Liu Shuo , Wang Jialu , Wang Yuhua , Shangguan Donghui
Published: January 2022

Traditional scenic route planning only considers the shortest path, which ignores the information of scenic road conditions. As the most effective direct search method to solve the shortest path in static road network, A* algorithm can plan the optimal scenic route by comprehensively evaluating the weights of each expanded node in the gridded scenic area. However, A* algorithm has the problem of traversing more nodes and ignoring the cost of road in the route planning. In order to bring better travel experience to the travelers, the above factors are taken into account. This paper presents a path planning method based on the improved A* algorithm. Firstly, the heuristic function of the A* algorithm is weighted by exponential decay to improve the calculation efficiency of the algorithm. Secondly, in order to increase the practicality of the A* algorithm, the impact factors that road conditions is introduced to the evaluation function. Finally, the feasibility of the improved A* algorithm is verified through simulation experiments. Experimental results show that the improved A* algorithm can effectively reduce the calculation time and road cost.
Diagnostic and therapeutic characteristics of diabetes mellitus and risk of out-of-hospital cardiac arrest
Authors: Park Jeong Ho , Ro Young Sun , Shin Sang Do , Cha Kyoung-Chul , Song Kyoung Jun , Hwang Sung Oh ,   For the phase II Cardiac Arrest Pursuit Trial with Unique Registry and Epidemiologic Surveillance (CAPTURES-II) investigators, Hwang Sung Oh , Shin Sang Do , Lee Mi Jin , Park Jong-Hak , Kim Su Jin , Oh Sung Bum , Shin Jonghwan , Park Seung Min , Sim Min Seob , Kim Won Young , Park In-Cheol , Ryu Hyun Ho , You Yeonho , Kim Sang-Chul , Park Ju Ok
Published: January 2022

This study aimed to evaluate the risks of diabetes mellitus (DM) on out-of-hospital cardiac arrest (OHCA) and to investigate whether the risks of DM on OHCA varied according to the diagnostic and therapeutic characteristics of diabetes. We conducted a multicenter prospective case–control study in 17 University hospitals in Korea from September 2017 to December 2020. Cases were EMS-treated OHCA patients aged 20 to 79 with a presumed cardiac etiology. Community-based controls were recruited at a 1:2 ratio after matching for age, sex, and urbanization level of residence. A structured questionnaire and laboratory findings were collected from cases and controls. Multivariable conditional logistic regression analyses were conducted to estimate the risk of DM on OHCA by characteristics. A total of 772 OHCA cases and 1544 community-based controls were analyzed. A total of 242 (31.3%) OHCAs and 292 (18.9%) controls were previously diagnosed with DM. The proportions of type I DM (10.7% vs. 2.1%) and insulin therapy (15.3% vs. 6.5%) were higher in OHCAs with DM than in controls with DM. The duration of DM was longer in OHCAs than in controls (median 12 vs. 7 years). DM was associated with an increased risk of OHCA (aOR (95% CI), 2.13 (1.64–2.75)). Compared to the no diabetes group, the risks of OHCA increased in the diabetes patients with type I DM (5.26 (1.72–16.08)) and type II DM group (1.63 (1.18–2.27)), a long duration of DM prevalence (1.04 (1.02–1.06) per 1-year prevalence duration), and a high HbA1c level (1.38 (1.19–1.60) per 1% increase). By treatment modality, the aOR (95% CI) was lowest in the oral hypoglycemic agent (1.47 (1.08–2.01)) and highest in the insulin (6.63 (3.04–14.44)) groups. DM was associated with an increased risk of OHCA, and the risk magnitudes varied according to the diagnostic and therapeutic characteristics.
Effect of vibration on orthodontic tooth movement in a double blind prospective randomized controlled trial
Authors: Mayama Atsushi , Seiryu Masahiro , Takano-Yamamoto Teruko
Published: January 2022

The purpose of the present study was to investigate the effect of vibration on orthodontic tooth movement and safety assessment based on our previous basic research in animal experiments. A double-blind prospective randomized controlled trial using split-mouth design was conducted in patients with malocclusion. The left and right sides of maxillary arch were randomly assigned to vibration (TM + V) and non-vibration (TM) groups. After leveling, vibrations (5.2 ± 0.5 g-forces (gf), 102.2 ± 2.6 Hertz (Hz)) were supplementary applied to the canine retracted with 100 gf in TM + V group for 3 min at the monthly visit under double-blind fashion, and the canine on the other side without vibration was used as TM group. The amount of tooth movement was measured blindly using a constructed three-dimensional dentition model. The amount of canine movement per visit was 0.89 ± 0.55 mm in TM group (n = 23) and 1.21 ± 0.60 mm in TM + V group (n = 23), respectively. There was no significant difference of pain and discomfort, and root resorption between the two groups. This study indicates that static orthodontic force with supplementary vibration significantly accelerated tooth movement in canine retraction and reduced the number of visits without causing side effects.
Stepwise slime mould growth as a template for urban design
Authors: Kay Raphael , Mattacchione Anthony , Katrycz Charlie , Hatton Benjamin D.
Published: January 2022

The true slime mould, Physarum polycephalum, develops as a vascular network of protoplasm, connecting node-like sources of food in an effort to solve multi-objective transport problems. The organism first establishes a dense and continuous mesh, reinforcing optimal pathways over time through constructive feedbacks of protoplasmic streaming. Resolved vascular morphologies are the result of an evolutionarily-refined mechanism of computation, which can serve as a versatile biological model for network design at the urban scale. Existing digital Physarum models typically use positive reinforcement mechanisms to capture meshing and refinement behaviours simultaneously. While these automations generate accurate descriptions of sensory and constructive feedback, they limit stepwise design control, reducing flexibility and applicability. A model that decouples the two “phases” of Physarum behaviour would enable multistage control over network growth. Here we introduce such a system, first by producing a site-responsive mesh from a population of nutrient-attracted agents, and then by independently calculating from it a flexible, proximity-defined shortest-walk to produce a final network. We develop and map networks within existing urban environments that perform similarly to those biologically grown, establishing a versatile tool for bio-inspired urban network design.
Swallowing kinematic analysis might be helpful in predicting aspiration and pyriform sinus stasis
Authors: Wei Kuo-Chang , Cheng Sheng-Hao , Hsiao Ming-Yen , Wang Yu-Chen , Weng Chi-Hung , Chen Jo-Yu , Wang Tyng-Guey
Published: January 2022

Aspiration due to dysphagia can lead to aspiration, which negatively impacts a patient’s overall prognosis. Clinically, videofluoroscopic swallow study (VFSS) is considered the gold-standard instrument to determine physiological impairments of swallowing. According to previously published literature, kinematic analyses of VFSS might provide further information regarding aspiration detection. In this study, 449 files of VFSS studies from 232 patients were divided into three groups: normal, aspiration, and pyriform sinus stasis. Kinematic analyses and between-group comparison were conducted. Significant between-group differences were noted among parameters of anterior hyoid displacement, maximal hyoid displacement, and average velocity of hyoid movement. No significant difference was detected in superior hyoid displacement. Furthermore, receiver-operating characteristic (ROC) analyses of anterior hyoid displacement, velocity of anterior hyoid displacement, and average velocity of maximal hyoid displacement showed acceptable predictability for detecting aspiration. Using 33.0 mm/s as a cutoff value of average velocity of maximal hyoid displacement, the sensitivity of detecting the presence of aspiration was near 90%. The investigators therefore propose that the average velocity of maximal hyoid displacement may serve as a potential screening tool to detect aspiration.
Dual isoform sequencing reveals complex transcriptomic and epitranscriptomic landscapes of a prototype baculovirus
Authors: Torma Gábor , Tombácz Dóra , Moldován Norbert , Fülöp Ádám , Prazsák István , Csabai Zsolt , Snyder Michael , Boldogkői Zsolt
Published: January 2022

In this study, two long-read sequencing (LRS) techniques, MinION from Oxford Nanopore Technologies and Sequel from the Pacific Biosciences, were used for the transcriptional characterization of a prototype baculovirus, Autographa californica multiple nucleopolyhedrovirus. LRS is able to read full-length RNA molecules, and thereby distinguish between transcript isoforms, mono- and polycistronic RNAs, and overlapping transcripts. Altogether, we detected 875 transcript species, of which 759 were novel and 116 were annotated previously. These RNA molecules include 41 novel putative protein coding transcripts [each containing 5′-truncated in-frame open reading frames (ORFs), 14 monocistronic transcripts, 99 polygenic RNAs, 101 non-coding RNAs, and 504 untranslated region isoforms. This work also identified novel replication origin-associated transcripts, upstream ORFs, cis-regulatory sequences and poly(A) sites. We also detected RNA methylation in 99 viral genes and RNA hyper-editing in the longer 5′-UTR transcript isoform of the canonical ORF 19 transcript.
Intrinsic cardiac adrenergic cells contribute to LPS-induced myocardial dysfunction
Authors: Yang Duomeng , Dai Xiaomeng , Xing Yun , Tang Xiangxu , Yang Guang , Harrison Andrew G. , Cahoon Jason , Li Hongmei , Lv Xiuxiu , Yu Xiaohui , Wang Penghua , Wang Huadong
Published: January 2022

Abstract

Intrinsic cardiac adrenergic (ICA) cells regulate both developing and adult cardiac physiological and pathological processes. However, the role of ICA cells in septic cardiomyopathy is unknown. Here we show that norepinephrine (NE) secretion from ICA cells is increased through activation of Toll-like receptor 4 (TLR4) to aggravate myocardial TNF-α production and dysfunction by lipopolysaccharide (LPS). In ICA cells, LPS activated TLR4-MyD88/TRIF-AP-1 signaling that promoted NE biosynthesis through expression of tyrosine hydroxylase, but did not trigger TNF-α production due to impairment of p65 translocation. In a co-culture consisting of LPS-treated ICA cells and cardiomyocytes, the upregulation and secretion of NE from ICA cells activated cardiomyocyte β1-adrenergic receptor driving Ca2+/calmodulin-dependent protein kinase II (CaMKII) to crosstalk with NF-κB and mitogen-activated protein kinase pathways. Importantly, blockade of ICA cell-derived NE prevented LPS-induced myocardial dysfunction. Our findings suggest that ICA cells may be a potential therapeutic target for septic cardiomyopathy.
Neurons learn by predicting future activity
Authors: Luczak Artur , McNaughton Bruce L. , Kubo Yoshimasa
Published: January 2022

Understanding how the brain learns may lead to machines with human-like intellectual capacities. It was previously proposed that the brain may operate on the principle of predictive coding. However, it is still not well understood how a predictive system could be implemented in the brain. Here we demonstrate that the ability of a single neuron to predict its future activity may provide an effective learning mechanism. Interestingly, this predictive learning rule can be derived from a metabolic principle, whereby neurons need to minimize their own synaptic activity (cost) while maximizing their impact on local blood supply by recruiting other neurons. We show how this mathematically derived learning rule can provide a theoretical connection between diverse types of brain-inspired algorithm, thus offering a step towards the development of a general theory of neuronal learning. We tested this predictive learning rule in neural network simulations and in data recorded from awake animals. Our results also suggest that spontaneous brain activity provides ‘training data’ for neurons to learn to predict cortical dynamics. Thus, the ability of a single neuron to minimize surprise—that is, the difference between actual and expected activity—could be an important missing element to understand computation in the brain.
Wildland fire smoke alters the composition, diversity, and potential atmospheric function of microbial life in the aerobiome
Authors: Kobziar Leda N , Vuono David , Moore Rachel , Christner Brent C , Dean Timothy , Betancourt Doris , Watts Adam C , Aurell Johanna , Gullett Brian
Published: January 2022

The atmosphere contains a diverse reservoir of microbes but the sources and factors contributing to microbial aerosol variability are not well constrained. To advance understanding of microbial emissions in wildfire smoke, we used unmanned aircraft systems to analyze the aerosols above high-intensity forest fires in the western United States. Our results show that samples of the smoke contained ~four-fold higher concentrations of cells (1.02 ± 0.26 × 105 m−3) compared to background air, with 78% of microbes in smoke inferred to be viable. Fivefold higher taxon richness and ~threefold enrichment of ice nucleating particle concentrations in smoke implies that wildfires are an important source of diverse bacteria and fungi as well as meteorologically relevant aerosols. We estimate that such fires emit 3.71 × 1014 microbial cells ha−1 under typical wildfire conditions in western US forests and demonstrate that wildland biomass combustion has a large-scale influence on the local atmospheric microbial assemblages. Given the long-range transport of wildfire smoke emissions, these results expand the concept of a wildfire’s perimeter of biological impact and have implications to biogeography, gene flow, the dispersal of plant, animal, and human pathogens, and meteorology.
Non-viral delivery of the CRISPR/Cas system: DNAversusRNAversusRNP
Authors: Lin Yi , Wagner Ernst , Lächelt Ulrich
Published: January 2022

Delivery of the CRISPR/Cas9 system can be realized by utilizing different biomolecular formats, each featuring individual characteristics, advantages and challenges.