bluuebunny/crossref_metadata_2025 huggingface dataset:
Peran Aglomer an Aglomerasi dalam P asi dalam Penyebaran Investasi Asing Langsung estasi Asing Langsung (FDI)
Authors:   Program Pascasarjana Ilmu Ekonomi Fakultas Ekonomi dan Bisnis Universitas Indonesia, Rejeki Indah Sri , Lubis Andi Fahmi ,   Program Pascasarjana Ilmu Ekonomi Fakultas Ekonomi dan Bisnis Universitas Indonesia
Published: May 2023

Bagi negara Indonesia dan negara-negara berkembang lainnya dimana tabungan domestic sangat terbatas, investasi asing langsung (foreign direct investment/FDI) menjadi salah satu primadona sumber pembiayaan pembangunan industri manufaktur. Permasalahan yang muncul adalah persebaran geografis FDI tidak merata antar provinsi di Indonesia. Penelitian ini menganalisis secara empiris peran aglomerasi dalam penyebaran investasi asing langsung (FDI) Industri Manufaktur Indonesia. Berbeda dengan penelitian sebelumnya, data FDI yang tersedia untuk tiap provinsi diagregasikan kedalam sub sektor 2 digit ISIC dari tahun 2010 – 2014. Hal ini dimaksudkan untuk memperkaya hasil penelitian sebelumnya, dimana sebagian besar mengasumsikan dampak aglomerasi adalah sama untuk semua sektor. Dengan menggunakan metode regresi Generalized Least Square (GLS), penelitian ini berhasil membuktikan adanya pengaruh posistif aglomerasi terhadap penyebaran FDI. Spesialisasi industri di suatu lokasi meningkatkan daya Tarik lokasi tersebut bagi masuknya FDI. Selain faktor aglomerasi, faktor-faktor lain yang mempengaruhi persebaran FDI di industri manufaktur Indonesia adalah PDRB per kapita, intensitas capital, tingkat upah, dan produktivitas tenaga kerja.
Posterior Hip Dislocation in Association With Anterior Column Acetabular Fracture, Femoral Head Fracture, and Sciatic Nerve Injury
Authors: Jarragh Ali , Almesbah Abdullrahman , Lari Ali
Published: May 2023

Case:

Posterior hip dislocations are classically associated with posterior acetabular wall fractures. We report the case of a 29-year-old man presented after a motorcycle accident with an unusual combination of injuries that included posterior hip dislocation, anterior column acetabulum fracture, femoral head fracture, and sciatic nerve injury. At the final follow-up, excellent outcomes were obtained with complete recovery of the sciatic nerve injury.

Conclusion:

A favorable outcome may be achieved in young patients who sustain this unusual compilation of ipsilateral anterior acetabulum fracture, posterior hip dislocation, femoral head fracture, and sciatic nerve injury with meticulous preoperative surgical planning and tailored patient management.
Corrective Osteotomy of Malunited Forearm Fractures for Volar Distal Radioulnar Joint Instability
Authors: Tsutsui Sadaaki , Okano Ichiro , Kuroda Takuma , Kawasaki Keikichi , Inagaki Katsunori
Published: May 2023

Case:

A 6-year-old girl who had midshaft forearm fractures treated conservatively had volar distal radioulnar joint (DRUJ) instability caused by radial malunion at the 1-year follow-up. Corrective osteotomy was planned using computer-aided design (CAD) software based on computed tomography images. According to the analysis, the radial bone had an 8° apex volar deformation in the sagittal plane. Corrective osteotomy was performed based on preoperative planning. After surgery, the patient regained full function of her right forearm without volar DRUJ instability.

Conclusion:

This case report shows that corrective osteotomy with 3D CAD analysis can help surgeons plan and accurately correct malunion.
Open Reduction and Internal Fixation of a Type IV Capitellum Fracture Through a Transolecranon Tunnel
Authors: Ulery David J. , Stegelmann Samuel D. , Phillips Seth A.
Published: May 2023

Case:

A 60-year-old woman sustained a type IV capitellum fracture from falling on an outstretched arm. Open reduction internal fixation (ORIF) was performed using an anconeus approach, and a transolecranon tunnel was created to place a trochlear screw. The patient showed good clinical outcomes with almost full range of motion at 6 months.

Conclusion:

With type IV capitellum fractures, the olecranon often obstructs the screw trajectory necessary for anterior-to-posterior fixation of trochlear fragments. Drilling a transolecranon tunnel through the proximal olecranon with the elbow flexed creates a viable pathway for screw placement from a more medial starting point than what is possible with traditional techniques.
The Effect of Application of Islamic Business Ethics and Islamic Service Quality on Customer Satisfaction of Bank Syariah Indonesia Madiun Branch Offices
Authors: Jannati Muzayinnatul , Nurhidayati Maulida
Published: May 2023

Islamic banks are closely related to customers. The existence of a bank is determined by the customer. So it becomes important to create customer satisfaction. In this case, the Indonesian Islamic Bank (BSI) Branch Office (KC) Madiun has tried to apply Islamic business ethics and Islamic service quality to the maximum, but there are customers who are not satisfied with this. The purpose of this study is to determine the effect on the application of Islamic business ethics, Islamic service quality, the effect of the application of Islamic business ethics and Islamic service quality on customer satisfaction. This type of research is quantitative associative. The data collection method used a questionnaire given to BSI KC Madiun customers. The sampling technique is incidental sampling with a sample size of 97 respondents. The data obtained were processed using SPSS 16. The analysis used was the classical assumption test, multiple regression test. The results of this study are the application of Islamic business ethics does not partially affect customer satisfaction BSI KC Madiun, Islamic service quality variable has a partial influence on customer satisfaction BSI KC Madiun. The variables of the application of Islamic business ethics and Islamic service quality have a simultaneous influence on customer satisfaction at BSI KC Madiun, which has a contribution of 47.5% and the rest is influenced by other variables that are not included in the model.
The Effectiveness of the Rida Embroidery Ponorogo Training and Course Institute (LPK) in Improving the Quality and Productivity of Trainees
Authors: Fauziah Nur , Prasetiyo Luhur
Published: May 2023

The Rida Embroidery Ponorogo Training and Course Institute (LPK) was formed to produce skilled and professional Human Resources who have an entrepreneurial spirit and are able to compete. However, is LPK Rida Embroidery Ponorogo really effective in improving the quality and productivity of the trainees themselves? This research is a field research using a qualitative approach with a descriptive type of research. Where the data collection uses interview, observation and documentation techniques. The resource persons in this study were the leadership of LPK Rida Embroidery Ponorogo, employees and training participants. Based on the findings in the field, the effectiveness of the Rida Embroidery Training Institute (LPK) in Ponorogo in improving the quality and productivity of training participants by using an effectiveness measure that is adjusted to the measurement of the quality and productivity of human resources is declared effective.
The Influence of the Family Environment, Entrepreneurial Knowledge, and Personality on Students' Entrepreneurial Interests at Student Boarding School al-Barokah Ponorogo
Authors: Romadona Laela , Suryaningsih Ruliq
Published: May 2023

The purpose of this study is to analyze the influence of the family environment, entrepreneurial knowledge and personality on students' entrepreneurial interests. This research is a type of quantative research. The population was 200 student respondents and a sample of 134 respondents. Meanwhile, the data collection technique carried out in this study was a questionnaire. The data analysis used is classical assumption test, simple linear regression analysis, multiple linear regression analysis, model accuracy test and hypothesis test. The results of this study show that the family environment positively affects the interest in entrepreneurship, entrepreneurial knowledge has a positive effect on entrepreneurial interests, personality negatively affects entrepreneurial interests, simultaneous results show the family environment, entrepreneurial knowledge and personality have a simultaneous effect on entrepreneurial interests.
Intimate partner violence among pregnant women in Addis Abeba, Ethiopia: Ignored antenatal care component
Authors:   1. Endalkachew Mekonnen Assefa,   2. Mukemil Tadele Aman
Published: May 2023

Background: Intimate partner violence (IPV) is a preventable public health problem and one of the most common types of violence against pregnant women which could be a cause of perinatal and maternal morbidities and mortalities. Screening for IPV during pregnancy provides an important window of opportunity for identifying pregnant women experiencing/ed IPV.
Objectives: The study aimed to assess the prevalence of intimate partner violence (IPV) and associated factors among the pregnant women attending the antenatal clinic in Addis Abeba Methods: A descriptive cross-sectional study was conducted on pregnant women attending ANC in selected health centers in Addis Abeba from the 1st of February 2019 to 30th of March 2019. A standardized validated WHO questionnaire was used to collect the data and it was analyzed by SPSS version 24. Descriptive statistical analysis was used to analyze the socio-demographic, prevalence of IPV, and chi-square and logistic regression with a P-value ≤ 0.05.
Results: Current and index pregnancy prevalence of intimate partner violence against pregnant women were found to be 55.7% (95% CI: 50.6-60.8) and 56.5% (95% CI: 51.4-61.5) respectively. Psychological violence was the most common form [53.4% (95%CI: 48.3-58.5)] followed by physical [13.5 %( 95% CI: 10.3-17.4)] then sexual violence 6.5 (95% CI: 4.3-9.5) during index pregnancy. The most frequent coexisted type of IPV during index pregnancy was psychological and physical which was 10.7% (95% CI: 7.8-14.2) followed by psychological and sexual 6.2 %( 95% CI: 4.0-9.2) Pregnant women who had primary level education (AOR 1.23(1.00-2.50)) and secondary level education (AOR 2.00(1.01-3.96)), women who had their first antenatal care (ANC) booking in the second trimester were about 1.7 times (AOR 1.74(1.2-2.85)) were more likely to experience IPV. Partner problematic alcohol consumption [AOR 4.36 (1.73-10.998)] P=0.001), Khat chewing [AOR 2.4(1.11-5.28)], partner educational level being primary [AOR 7.02 (3.29-15.2)] and secondary 5 times (2.38-11.45) and, history of IPV before pregnancy were independently associated with increased risk of intimate partner violence during the index pregnancy.
Conclusion & Recommendation: More than half of the pregnant women during the index and current pregnancy experienced at least one form of IPV. Women's and partners’ education status, gestational age at ANC booking, and partner's problematic alcohol drinking and khat chewing habits were independent determinants of intimate partner violence during pregnancy. We recommend health service providers should implement the WHO's clinical guidelines for IPV screening amongst women attending antenatal care since IPV is more prevalent.
Effect of long-term treatment of ulipristal acetate on rat ovarian tissue
Authors: Hirano Mana , Wada-Hiraike Osamu , Fukui Motoko , Shibata Seiji , Uehara Mari , Nagumo Aiko , Urata Yoko , Sone Kenbun , Harada Miyuki , Koga Kaori , Osuga Yutaka
Published: May 2023

Ulipristal (UPA), a selective progesterone receptor modulator, has both agonistic and antagonistic effects on progesterone receptors. UPA suppresses ovulation by inhibiting the luteinizing hormone (LH) surge from the pituitary gland; however, the direct effect of UPA on ovarian tissue remains poorly studied. In the present study, we examined the effects of UPA on the ovaries of rats. Rats were treated for 28 d with 4, 20, and 100 mg/kg UPA. UPA treatment increased the number of primordial follicles at each treatment group, with the highest number found in the 4 mg/kg group, and the number of primordial follicles decreasing with increasing dose. The number of primary and antral follicles tended to increase with increasing UPA levels. Furthermore, the decrease in primary follicle number could be attributed to the exhaustion of follicles, but the examination of proliferation markers, oxidative stress markers, and cell death markers revealed no remarkable toxic effects on ovarian tissues. These results suggest that UPA treatment promotes follicle development at each stage but inhibits ovulation by suppressing the LH surge, resulting in an increase in atretic follicles or unruptured luteinized cysts. UPA may not have toxic effects on the ovary because the expression of antioxidant genes and cell death markers was not dramatic in follicles treated with UPA. Taken together, these results suggest that UPA may not have a direct local effect on ovarian follicles. Hence, we hypothesized that prolonged UPA treatment in patients with uterine fibroids may not be harmful and may not decrease future fecundity.
Metformin significantly reduced remnant cholesterol in patients with newly diagnosed type 2 diabetes mellitus after 48-week treatment
Authors: jingru Su , Zhou liyuan , Yang Ning , Xu Yuan , Wang Guang , Liu Jia
Published: May 2023

Background
Metformin has become the first-line therapy for type 2 diabetes mellitus (T2DM) and has beneficial effects on cardiovascular disease (CVD). Remnant cholesterol (RC), the cholesterol of triglyceride-rich lipoproteins, is associated with the risk of CVD. Our research group first explored the relationship between RC and metabolic disorders in China. However, it is still unclear whether metformin could decrease RC. This study aims to investigate the effect of metformin monotherapy on RC in patients with new-onset T2DM.
Methods
This study was nested in an open-label, randomized trial (MARCH), which was designed to assess the effectiveness of acarbose compared with metformin as the initial therapy for new-onset T2DM. All individuals were diagnosed with T2DM within the past 12 months and had not received any therapy of oral hypoglycemic drugs or lipid-lowering drugs. The primary endpoint was the changes of RC after 48-week metformin treatment. Additionally, we compared alterations in RC among sub-groups divided by the predictor of CVD-atherogenic index of plasma (AIP), low-density lipoprotein cholesterol (LDL-C), body mass index, or hemoglobin A1c.
Results
After 48-week treatment of metformin, in addition to total cholesterol, triglyceride, LDL-C, RC was also decreased (all P < 0.05) in patients with new-onset T2DM, while the reduction of RC was the most prominent in patients with the highest tertile of AIP. A remarkable decline of RC was observed in both LDL-C < 2.6 mmol/L and LDL-C ≥ 2.6 mmol/L groups, which was greater in participants with LDL-C < 2.6 mmol/L (P < 0.05).
Conclusions
This study demonstrated that besides improving glucose and conventional lipid profiles, 48-week metformin treatment significantly decreased RC in patients with newly diagnosed T2DM, especially in those with high cardiovascular risk evaluated by AIP and those with well-controlled LDL-C. The results expand the clinical effect of metformin and provide evidence for its clinical usage.
Trial Registration
ChiCTR.org ChiCTR-TRC-08000231
Distribution of antibiotic resistance gene against Streptococcus parauberis isolated from cultured olive flounder (Paralichthys olivaceus) in Jeju, South Korea
Authors: Kim Ye Ji , Jun Lyu Jin , Lee Young Juhn , Han Ji Eun , Kim Sung Hyun , Jeong Joon Bum
Published: May 2023

Common bacterial diseases in the olive flounder Paralichthys olivaceus, a commercially important fish species, are caused by Streptococcus spp., Vibrio spp., and Edwardsiella piscicida, which cause significant economic losses in aquaculture. Antibiotics are mainly used to treat bacterial diseases. However, continued use of antibiotics causes bacterial resistance, which may lead to the transfer of antibiotic resistance genes. In our study, we isolated bacteria from the livers of cultured olive flounder from 34 farms on Jeju Island, South Korea. A total of 611 bacteria were isolated, of which 296 (48%) were Streptococcus parauberis by polymerase chain reaction (PCR) analysis. The isolates were tested for antibiotic susceptibility to 17 disks (including ampicillin, amoxicillin, erythromycin, tetracycline, oxytetracycline, and doxycycline) using disk diffusion. In addition, 13 antibiotic resistance genes were tested, including 9 tetracycline resistance genes (including tet(B) and tet(M)) and 4 macrolide resistance genes (including erm(B)). Data analysis of resistance levels were analyzed using normalized resistance interpretation (NRI) and multiple antibiotic resistance (MAR) index based on inhibition zones derived from the disk diffusion method. Antibiotic resistance genes were detected in all 296 isolates of S. parauberis. Tetracycline resistance genes were detected in 116 strains (39%) and macrolide resistance genes were identified in 96 strains (32%). The analysis of NRI resulted in COwt values for 13 antibiotics, excluding the COwt values for the four antibiotics sulfadiazine, oxolinic acid, nalidixic acid, and flumequine, which did not exhibit a normal distribution due to the dominance of non-wild types. Analysis of the multiple antibiotic resistance (MAR) showed that 39.9% of S. parauberis isolates had an MAR index of 0.22. The cut-off values presented in this study can be used as reference values for the disk diffusion method results because the experiment was performed on many S. parauberis. Future studies on antibiotic resistance of various fish pathogens should be conducted, with emphasis on alternate treatments.
Microplastic pollution in Kolavai Lake, Tamil Nadu, India: Quantification of plankton-sized microplastics in the surface water of lake
Authors: Rajeswari Ineyathendral Thandavamoorthy , Iyyanar Anandavelu , Govindarajulu Bavani
Published: May 2023

The prevalence of microplastics (< 5 mm) in aquatic habitats has recently raised concerns owing to their influence on humans and aquatic organisms, as they absorb organic pollutants and pathogens from the surrounding media because of their higher surface-to-volume ratio. Freshwater systems are severely affected by the increased intake of discarded waste from diverse sources. This study focused on the microplastic-to-zooplankton ratio and its potential impact on the environment's food chain. The sampling sites of Kolavai Lake were divided into three zones (18 stations) to investigate the spatial distribution of microplastics and zooplankton biota. The average microplastic abundance was 6.1 ± 2.5 particles/L. Fourier Transform Infrared Spectroscopy (FTIR) and SEM analysis were performed to understand the chemical composition and surface morphology of microplastics. Water samples collected along the Central and Southern Zones revealed a high abundance of microplastics, which might be due to anthropogenic activities. A negative correlation was observed between the abundance of microplastics and zooplankton. The microplastic-to-zooplankton ratio was found to range from 0.05 to 0.74. Further, the impact of microplastics in the lake ecosystem was analysed using the size and shape descriptors for both zooplankton and microplastics. These findings suggest that microplastics built up in aquatic environments, particularly those with rich biota, could be a severe concern because of their capacity to infiltrate the food web.
Reactive response to predation risk affects foraging time of hares, yet not their phosphorus intake
Authors: Brandsen Samara , Vermorken Louise Sophia , Kuipers Henry , Wieren Sip van , de Jonge Inger , Weterings Martijn
Published: May 2023

Antipredator responses could affect nutrient intake, which could lead to nutritional deficits. However, little is known about the antipredator response of small herbivores because most are nocturnal or crepuscular and therefore very difficult to study in the field. Therefore we experimentally assessed the effect of a reactive response to predation risk on the nutrient (i.e., phosphorous) intake of the European hare (Lepus europaeus) by using three different playback sounds. Additionally, we studied the time spent being costly vigilant, the time spent foraging, and the vegetation height in which the hares were present by using accelerometers and GPS. Our results showed that elevated predation risk from our playback experiment did not affect the 1) phosphorus intake, 2) time spent being costly vigilant, and 3) time spent in tall vegetation. However, elevated predation risk did increase the time spent foraging. Possibly hares spent more time foraging with an increased predation risk because hares cannot seek refuge from predators. Additionally, the effect on phosphorus intake could be weak because phosphorous intake does not benefit a flight escape, while the reactive response acts late in the predation sequence limiting the effect on hare ecology. Prey anti-predator responses seem strongly related to the escape tactics of prey species that can differ between different habitats and the time of the day. More detailed field studies are necessary to get a better insight into species’ anti-predator-food tactics.
Association between Anticoagulation Strategy and Quality of Life in Chronic Hemodialysis Patients
Authors: Eloot Sunny , Vanommeslaeghe Floris , Josipovic Iván , Boone Matthieu , Biesen Wim Van
Published: May 2023

Prevention of clotting in hemodialysis (HD) is a concern, but tools to monitor anticoagulation strategies as well as data on bleeding and its impact on quality of life (QoL) are scant. In this prospective longitudinal observational study, bleeding tendency in 70 HD patients was scored with ISTH-BAT and HAS-BLED at week 0, 4, and 8. Patient’s limbs were visually scored for bruises and hematomas, and Quality of Life (QoL) was assessed using EQ5D-3L and Visual Analogue Scale (VAS) questionnaires. At week 0, the used hemodialyzer was scanned in a micro-CT scanner to quantify the number of patent fibers. Bleeding scores were 0[0;1] and 3[2;4] for ISTH-BAT and HAS-BLED, and visual scoring showed 2[0;4] bruises/hematomas. QoL was 0.85[0.77;1.00] for EQ5D and 70[60;80] for VAS. Fiber patency was 81[70;90]%, but was not associated with anticoagulation dose (p = 0.103). Patients in the highest tertile of anticoagulation dose had a worse VAS score (p = 0.027), and patients identified as having bleeding tendency by ISTH also had a worse VAS score (p = 0.010). Thus, in maintenance HD patients, dialyzer fiber blocking is rather limited, but the substantial degree of minor bleeding suggests that current anticoagulation doses are potentially too exaggerated, negatively impacting QoL.
The children of the opioid epidemic: A mixed method examination of the developmental implications of prenatal opioid exposure among school-aged children
Authors: Wilkinson Andra L. , Rackers H. S. , Harmon T
Published: May 2023

Background
Growing research points to potential long-term developmental implications of prenatal opioid exposure for children. Yet, polysubstance use and adverse childhood experiences are raised as potential confounders. Further, there is a lack of data on school-age children and the children’s strengths.
Methods
Parents and caregivers of children with prenatal opioid exposure worked with the study team to design, collect, and descriptively analyze mixed method data. Data were collected through survey (n = 148) and focus groups (n = 15) from a convenience sample in mostly West Virginia and Massachusetts.
Results
Eighty percent of children with prenatal opioid exposure in the sample were diagnosed with NOWS. Nearly half of the children in the sample were diagnosed with multiple developmental delays, behavioral health conditions, and specific learning disorders. Roughly 85% of children have behavioral challenges. Importantly, over 80% of families also reported their child’s strengths, including empathy, social magnetism, and their resilience.
Conclusions
The challenges for children born with prenatal opioid exposure may extend into school-age. The results are consistent with prior research on younger children, suggesting a potential need for the best practices for caring for neonates with NOWS to be extended to further ages and developmental stages.
Excitatory Nerve Conduction Changed In a Partial BOO-induced OAB Rat Model
Authors: Huang Jingyi , Li Hongliang , Zhang Yao , Liu Jiaye , Cao Hongying , Long Yongling
Published: May 2023

Background
Overactive bladder (OAB) is a common, long-term symptom complex with a high prevalence in women worldwide. The pathogenesis of OAB has not been elucidated. This study investigated the excitatory purinergic and cholinergic nerve conduction in OAB rat model induced by partial bladder outlet obstruction(PBOO）.
Methods
Model rats underwent bladder outlet obstruction surgery. In the 2nd, 3rd, and 4th week after surgery, metabolic cages were used to detect the 12-hours Urine volume of the rats in the sham and model groups. The urodynamic parameters Bladder leak point pressure (BPLL), Maximum voiding pressure (MVP), Residual volume (RV), Maximum bladder capacity (MBC), Bladder compliance (BC), Voided efficiency (VE), and Non-voiding contractions (NVCs) were also detected. Besides, the time point above examined the contractile responses of isolated detrusor muscles to electrical and carbachol stimulation.
4th week after surgery, the bladders of both groups were taken for hematoxylin, eosin (H&E), and Masson’s trichrome staining. Besides, real-time qPCR and Western blot were performed to quantify the expression of Choline acetyltransferase(ChAT) and Solute carrier family 17 member 9 (SLC17A9).
Results
At week 4, compared with the sham group, the 12 h Urine volume increase significantly. The BLPP、MVP、VE、MBC、NVCs increased significantly, and the VE was significantly reduced. The contractile responses of isolated detrusor muscles to electrical and carbachol stimulation were significantly increased.
In 4 week PBOO group, the bladder wall and the ratio of bladder muscle to collagen within the bladder smooth muscle layer wall were significantly higher than in the sham group. ChAT and SLC17A9 mRNA and protein expression in OAB model rats was significantly increased.
Conclusions
4 weeks after PBOO, the OAB model was successfully established. The gene and protein expressions of ChAT and SLC17A9 were increased in the bladder of OAB model, suggesting that OAB may relate to the increased excitatory purinergic and cholinergic nerve conduction.
Antibacterial mechanism of garviecin LG34 against S. aureus and L. monocytogenes and its application in dairy products preservation
Authors: Gao Yurong
Published: May 2023

Purpose The objective of this paper was to identify the antibacterial mode of garviecin LG34 against S. aureus and L. monocytogenes and the inhibitions on these two food-borne pathogenic bacteria in dairy products.Methods Antibacterial mechanism of garviecin LG34 was identified by the effects on the efflux of Potassium (K+) ions, extracellular electrical conductivity, UV-absorbing substances, potential across the membrane (ΔΨ) and cell permeability. The inhibitions of garviecin LG34 against S. aureus and L. monocytogenes in milk was studied by plate colony counting method.Results Supplementation with 160 AU/ml of garviecin LG34 had a bactericidal effect on these two bacteria. 160 AU/ml of garviecin LG34 resulted in the effusion of potassium ion and UV-absorbing substances, the leakage of cellular electrolytes and the dissipation of electrical potential across the membrane of these two food-borne bacteria. Moreover, the increase in cell permeability of both strains was observed by flow cytometer after cells treated with 160 AU/ml of garviecin LG34. Garviecin LG34 significantly inhibited the growth of these two food-borne bacteria. in the dairy products especially in skim milk.Conclusion Garviecin LG34 could cause pore formation, intracellular materials release and permeability increase of S. aureus and L. monocytogenes, and may be applied to dairy products as bio-preservative.
Subretinal delivery of GMP-grade human neural progenitor cells protect vision in rat model of retinal degeneration and survive in minipigs
Authors: Lu Bin , Avalos Pablo , Svendsen Soshana , Zhang Changqin , Nocito Laura , Jones Melissa K , Pieplow Cosmo , Saylor Joshua , Ghiam Sean , Block Amanda , Fernandez Michael , Ljubimov Alexander V , Small Kent , Liao David , Svendsen Clive N , Wang Shaomei
Published: May 2023

Background: Stem cell products are increasingly entering early stage clinical trials for treating retinal degeneration. The field is learning from experience about comparability of cells proposed for preclinical and clinical use. Without this, preclinical data supporting translation to a clinical study might not adequately reflect the performance of subsequent clinical-grade cells in patients.
Methods: Research- grade human neural progenitor cells (hNPC) and clinical-grade hNPC (termed CNS10-NPC) were injected into the subretinal space of the Royal College of Surgeons (RCS) rats, a rodent model for retinitis pigmentosa (RP); An IND-enabling study with CNS10-NPC was perform in the same rodent model; Finally, surgical methodology for subretinal cell delivery in the clinic was optimized in large animal model-Yucatan minipig.
Results: Both research grade hNPC and clinical-grade hNPC (termed CNS10-NPC) can survive and provide functional and morphological protection in a dose-dependent fashion in the RCS rats and defined the optimal cell dose used for an investigational new drug (IND) enabling study. Grafted CNS10-NPC migrated from the injection site without differentiation into retinal cell phenotypes. Additionally, CNS10-NPC showed long-term survival, safety and efficacy in a toxicity and tumorigenicity study, with no observed cell overgrowth even at the maximum deliverable dose. Finally, using a large animal model-Yucatan minipig, which has eye size comparable to the human, we optimized the surgical methodology for subretinal cell delivery in the clinic.
Conclusions: These extensive studies supported an approved IND and the translation of CNS10-NPC to an ongoing Phase 1/2a clinical trial (NCT04284293) for the treatment of retinitis pigmentosa.
Assessment of the superior photocatalytic properties of Sn2+-containing SnO2 microrods on the photodegradation of methyl orange
Authors: Jorgetto Alexandre de Oliveira , Zanoni Maria Valnice Boldrin , Orlandi Marcelo
Published: May 2023

A Sn2+-containing SnO2 material was synthesized via a simple hydrothermal route, and its characterization demonstrated that it presented a microrod morphology with rutile SnO2 crystalline structure. Sn2+ ions were detected in the interior of the material and no other impurities, such as Sn2+-containing tin oxide phases (Sn2O3, Sn3O4, SnO) were detected. The material’s optical properties have shown the absorption of a considerable fraction of visible light up to wavelengths of 671 cm-1, contrastingly to ordinary SnO2. The analysis of the internal crystalline structure of a single microrod was carried out with a Focused Ion beam microscope, confirming that the material was highly defective with strong polycrystalline nature down to the nanoscale. The results indicated that the constituent Sn2+ ions occupy intergranular sites in a rutile SnO2 mesocrystalline structure, and that Sn2+ states were responsible for the material’s superior photoactivity. The photocatalytic performance of the material was much higher than those of commercial TiO2 and SnO2 materials, and it decomposed nearly all methyl orange dye content in water (10 mg L‑1) in 6 min under UV irradiation for a photocatalyst dose of 5.33 g L‑1. The photodegradation of methyl orange was also verified under visible light.
Mechanical and Thermal Characterization of Polyurethane Resin- based Composite Material Reinforced with Dry Nanocellulose using Vacuum Casting
Authors: Jadhav Shraddha , Utikar Vedant , Sonawane Pavankumar , Jain Soham , Bhadekar Priti
Published: May 2023

The purpose of this research is to examine the mechanical and thermal characteristics of new class of composite material. The novel composite has been obtained by adding dry nanocellulose in mixture of polyurethane resin and hardener. A biodegradable, sustainable substance called nanocellulose has special mechanical, optical, and barrier properties. Nanocellulose has the potential to be a green nanomaterial because of its availability, sustainability and biodegradability. Vacuum casting is a process that produces high-quality castings that are bubble-free, have a smooth surface texture, and are flawless. The polyurethane resin and hardener are combined at 35°C and then poured the mixture into a vacuum casting machine and it allowed to cure for 20 minutes. The investigation includes samples with weight percentages of 0, 2, 4, 6, and 8%. Tensile, Izod Impact, Hardness (Shore D), and Differential Scanning Calorimetry (DSC) tests were performed. Test revealed that addition of dry nanocellulose making the composite brittle. Results suggest that adding dry Nanocellulose in high strength resin reduces overall strength, while literature shows that adding wet Nanocellulose improves properties like impact, tensile strength, but reduces thermal properties. The sample with 4% of Nanocellulose (filler material) performs better in terms of characteristics and yields better results than the other percentages. However, dry nanocellulose cannot be used in ultra-high strength applications due to its potential to reduce overall strength.
The co-design of a new surveillance protocol for thoracic oncology patients
Authors: Fraser Anne , McNeill Rob , Robinson Jackie
Published: May 2023

Lung cancer patients with advanced disease and no active treatment options currently face frequent follow-up visits to the outpatient clinic; associated with significant anxiety, time commitment, and costs. These visits also place considerable strain on the health system. Evidence from other population groups (including people with some cancers and chronic health conditions) suggests virtual or remote follow-up can lead to higher patient satisfaction without negatively impacting on health outcomes such as survival time. The aim of this study was to identify preferences for different surveillance protocols for people with non-curative lung cancers or mesothelioma. A three-phase co-design approach was adopted, including peg boards, semi-structured interviews and a discrete choice experiment (DCE). Findings from the DCE identified a need for reassurance, and the importance of relationship, consistency, and convenience for patients. Participants also identified that their preference for follow-up would be with an oncologist based locally at their primary care practice. Nurse-led and telehealth clinics were also considered acceptable options. Findings informed the design of a feasibility trial of a novel surveillance approach.
A novel blended and interprofessional approach to pediatric emergency training: self-assessment, perception, and perceived long-term effects
Authors: Lehmann Ronny , Petrowsky Michaela Klinke , Seitz Anke , Meyburg Jochen , Eppich Walter , Hoffmann Georg F. , Tönshoff Burkhard , Huwendiek Sören
Published: May 2023

Background: We developed a blended training program at a tertiary pediatric center based on hospital-specific emergency guidelines, profession-specific Virtual Patients (VPs), and interprofessional team training. Using this novel approach, we addressed differing educational needs of medical and nursing staff and intrinsic cognitive overload among participants, aiming for harmonization of in-house emergency proceedings.
Methods: Self-assessments of pediatric emergency knowledge and skills were conducted before (T1) and after (T2) preparation using VPs, as well as after the team training day (T3). At T3, participants completed questionnaires on the training approach, its components, and learning impact. Ten months after the training, a follow-up survey (T4) queried perceived benefits within and beyond emergency situations.
Results: A total of 56 medical staff and 56 nursing staff members participated in the pilot phase. Of these, N=55 (98%) and N=48 (85%), respectively, returned self-assessments; questionnaires were completed by N=55 (98%) and N=51 (91%), respectively. In both groups, 57 participants (50.9%) completed the follow-up survey. After team training (T3), both groups had significantly increased knowledge and skill scores compared with those at T1. Regarding the blended approach and its components, medical and nursing staff alike rated the entire course and its guidelines, the preparatory VPs, and the team training very highly. Participants felt being better prepared for pediatric emergencies. Perceived strengths of the training approach were in the triangulation of teaching methods and its inter-professionalism. More training scenarios were requested, as well as recurrent training. In the follow-up, participants reported improved confidence and calmness, as well as improved communication and collaboration when involved in an emergency. Beyond emergencies, benefits were reported in daily routines.
Conclusions: Our blended approach was perceived as being effective in improving preparedness among medical and nursing house staff. This approach permits customization of content and deliberate practice to improve pediatric critical care.
Comparison of Efficacy of Hyperthermic Intraperitoneal Chemotherapeutics Mitomycin C versus Oxaliplatin In Experimental Colorectal Peritoneal Metastasis Model
Authors: Manoğlu Berke , Canda³ Aras Emre , Yavuzşen² Tuğba , Aktaş³ Safiye , Altun³ Zekiye , Yılmaz⁴ Osman , Bişgin¹ Tayfun
Published: May 2023

Aim
Our study aimed to establish a mouse model with colorectal cancer-induced peritoneal metastasis(PM) and to compare the efficacy of hyperthermic intraperitoneal chemotherapeutic agents, mitomycin C and oxaliplatin.
Materials and Methods
The peritoneal metastasis model was established in nude mice using the CC531 colon carcinoma cell line. Models with PM were randomized into four groups of seven animals each: Group 1, control group; Group 2, hyperthermic intraperitoneal chemotherapy(HIPEC) with mitomycin C (MMC), and Group 3, HIPEC with Oxaliplatin(OXA).
Results
Tumor development was achieved in all animals. While the tumor burden decreased significantly in the treatment Group 2(p = .013). In the PM mouse model, hyperthermic intraperitoneal administration of MMC had a higher tumoricidal effect than hyperthermic intraperitoneal administration of OXA.
Conclusions
Our PM model provided a good opportunity to examine the efficacy of HIPEC and IPIP. Hyperethermic intraperitoneal mitomycin applied in the colorectal PM animal model was found to have higher tumoricidal activity than oxaliplatin. In future studies, we plan to evaluate efficacies of different drugs in the PM models we have created.
Higher Neuronal Facilitation and Potentiation with APOE4 Suppressed by Angiotensin II
Authors: Scheinman Sarah B. , Tseng Kuei Y. , Alford Simon , Tai Leon M
Published: May 2023

Progressive hippocampal degeneration is a key component of Alzheimer’s disease (AD) progression. Therefore, identifying how hippocampal neuronal function is modulated early in AD is an important approach to eventually prevent degeneration. AD-risk factors and signaling molecules likely modulate neuronal function, includingAPOEgenotype and angiotensin II. Compared toAPOE3,APOE4increases AD risk up to 12-fold, and high levels of angiotensin II are hypothesized to disrupt neuronal function in AD. However, the extent thatAPOEand angiotensin II modulates the hippocampal neuronal phenotype in AD-relevant models is unknown. To address this issue, we used electrophysiological techniques to assess the impact ofAPOEgenotype and angiotensin II on basal synaptic transmission, presynaptic and post-synaptic activity in mice that express humanAPOE3(E3FAD) orAPOE4(E4FAD) and overproduce Aβ. We found that compared to E3FAD mice, E4FAD mice had lower basal synaptic activity, but higher levels of paired pulse facilitation (PPF) and Long-Term Potentiation (LTP) in the Schaffer Collateral Commissural Pathway (SCCP) of the hippocampus. We also found that exogenous angiotensin II has a profound inhibitory effect on hippocampal LTP in both E3FAD and E4FAD mice. Collectively, our data suggests thatAPOE4and Aβ are associated with a hippocampal phenotype comprised of lower basal activity and higher responses to high frequency stimulation, the latter of which is suppressed by angiotensin II. These novel data suggest a potential mechanistic link between hippocampal activity,APOE4genotype and angiotensin II in AD.
Bifurcation analysis of a tuberculosis progression model for drug target identification
Authors: Flores-Garza Eliezer , Hernández-Pando Rogelio , García-Zárate Ibrahim , Aguirre Pablo , Domínguez-Hüttinger Elisa
Published: May 2023

Tuberculosis (TB) is a major cause of morbidity and mortality worldwide. The emergence and rapid spread of drug-resistant M. tuberculosis strains urge us to develop novel treatments. Experimental assays are restricted by lab capacity, insufficient funds, low numbers of laboratory animals and obsolete technology. Systems-level approaches to quantitatively study TB can overcome these limitations. Previously, we proposed a mathematical model describing the key regulatory mechanisms underlying the pathological progression of TB. Here, we systematically explore the effect of parameter variations on disease outcome. We find five bifurcation parameters that steer the clinical outcome of TB: number of bacteria phagocytized per macrophage, macrophages death, macrophage killing by bacteria, macrophage recruitment, and phagocytosis of bacteria. The corresponding bifurcation diagrams show all-or-nothing dose-response curves with parameter regions mapping onto bacterial clearance, persistent infection, or history-dependent clearance or infection. Importantly, pathogenic stage strongly affects the host’s sensitivity to these parameter variations. We identify parameter values corresponding to a latent-infection model of TB, where disease progression occurs significantly slower than in progressive TB. Two-dimensional bifurcation analyses uncovered synergistic parameter pairs which could act as efficient compound therapeutic approaches. Through bifurcation analysis, we reveal how modulating specific regulatory mechanisms could steer the clinical outcome of TB.
Induction hepatic arterial infusion chemotherapy with concurrent radiotherapy followed by surgery for hepatocellular carcinoma with massive portal vein tumor thrombosis
Authors: Kimura Yuta , Tokumitsu Yukio , Shindo Yoshitaro , Matsui Hiroto , Matsukuma Satoshi , Saeki Issei , Takami Taro , Yamasaki Takahiro , Ioka Tatsuya , Nagano Hiroaki
Published: May 2023

Background
The prognosis of hepatocellular carcinoma (HCC) with portal vein tumor thrombosis (PVTT) is very poor, and the optimal treatment remains controversial. The aim of this study was to examine the safety and feasibility of our multimodal treatment.
Methods
This was a single-institution, retrospective case series. From 2013 to 2018, induction hepatic arterial infusion chemotherapy (HAIC) with concurrent radiotherapy (RT) was given to 20 consecutive patients with HCC harboring PVTT in the main portal trunk or first branch, even with intrahepatic and extrahepatic metastasis. When the cancers including thrombus and metastatic disease were well-controlled, surgical resection was considered. When macroscopic complete resection was achieved, two courses of HAIC were added as adjuvant therapy, whereas patients who had remnant disease after surgery were provided treatment according to the type of lesion.
Results
No treatment-related deaths were noted. The objective response rate and disease control rate were 35.0% and 65.0%, respectively. After induction treatment, 10 of 20 patients underwent surgery. Postoperative complications (Clavien-Dindo Grade III or more) were observed in three cases, and median postoperative hospital stay was 15.5 days. Median survival time of all 20 patients was 14.5 months, and that in patients who underwent surgery was significantly longer than that in patients with unresectable HCC (19.5 months vs 9.0 months, p = 0.0018).
Conclusions
Induction treatment followed by surgery was safe and feasible for HCC with massive PVTT. Surgical resection might be oncologically appropriate for selected patients after induction treatment even with advanced stage HCC.
Deficiency of acetyltransferase nat10 in zebrafish causes developmental defects in the visual function and behavior
Authors: Yang Hou-Zhi , Zhuo Donghai , Huang Zongyu , Luo Gan , Liang Shuang , Fan Yonggang , Zhao Ying , Lv Xinxin , Qiu Caizhen , Zhang Lingzhu , Liu Yang , Sun Tianwei , Chen Xu , Li Shan-Shan , Jin Xin
Published: May 2023

N4-acetylcytidine (ac4C) is an epitranscriptomic modification of mRNA that is catalyzed by N-acetyltransferase 10 (NAT10), a critical factor known to influence mRNA stability. However, its role in development has not been investigated. In this study, we used CRISPR/Cas9 and RNAi technology to knock out and knock down nat10, the zebrafish ortholog of human NAT10, and evaluated their effects on development, behavior, and transcriptome. Our findings indicate that nat10 deficiency in zebrafish embryos results in increased embryo mortality and developmental abnormalities. Additionally, behavioral and histological evaluations revealed that nat10 knockdown led to increased anxiety-like behavior and severe vision impairment. Transcriptome profiling and RT-PCR results showed that nat10 knockdown significantly downregulated the expression of retinal transcripts that are enriched in response to light stimuli, photoreceptors, and visual perception. Furthermore, dot-blot and RIP-PCR analyses confirmed a significant reduction in ac4C levels in total RNA and opsin mRNA in nat10 knockdown zebrafish. Our results highlight the essential role of ac4C in embryonic development, especially in visual development. This zebrafish model could be helpful for studying ac4C modification in neurodevelopmental disorders.
Quantum and Relativistic corrections to Maxwell-Boltzmann ideal gas model from a Quantum Phase Space approach
Authors: Ravelonjato Rivo Herivola Manjakamanana , Ranaivoson Ravo Tokiniaina , Andriambololona Raoelina , Raboanary Roland , Rakotoson Hanitriarivo , Rabesiranana Naivo
Published: May 2023

The quantum corrections related to the ideal gas model that are often considered are those which are related to the particles nature: bosons or fermions. These corrections leadrespectively to the Bose-Einstein and Fermi-Dirac statistics. However, in this work, otherkinds of corrections which are related to the quantum nature of phase space are considered. These corrections are introduced as improvement in the expression of the partition function of an ideal gas. Then corrected thermodynamics properties of the gas are deduced. Both the non-relativistic quantum and relativistic quantum cases are considered. It is shown that the corrections in the non-relativistic quantum case may be particularly useful to describe the deviation from classical behavior of a Maxwell-Boltzmann gas at low temperature and in confined space. These corrections can be considered as including the description of quantum size and shape effects. For the relativistic quantum case, the corrections could be relevant for confined space and when the thermal energy of each particle is comparable to their rest energy. The corrections appear mainly as modifications in the thermodynamic equation of state and in the expressions of the partition function and thermodynamic functions like entropy, internal energy, and free energy. Classical expressions are obtained as asymptotic limits.