bluuebunny/crossref_metadata_2025 huggingface dataset:
Development and Experimental Study of Supercritical Flow Payload for Extravehicular Mounting on TZ-6
Authors: Guo Liang , Duan Li , Zou Xuemei , Gao Yang , Zhang Xiang , Su Yewang , Wang Jia , Wu Di , Kang Qi
Published: October 2024

This paper provides a detailed description of the development and experimental results of the supercritical flow experiment payload carried on the TZ-6 cargo spacecraft, as well as a systematic verification of the out-of-cabin deployment experiment. The technical and engineering indicators of the payload deployment experiment are analyzed, and the functional modules of the payload are shown. The paper provides a detailed description of the design, installation location, size, weight, temperature, illumination, pressure, radiation, control, command reception, telemetry data, downlink data, and experimental procedures for the out-of-cabin payload in the extreme conditions of space. The paper presents the annular liquid surface state and temperature oscillation signals obtained from the space experiment and conducts ground matching experiments to verify the results, providing scientific references for the design and condition setting of space experiments and comparisons for the experimental results to obtain the flow field structure under supercritical conditions. The paper provides a specific summary and discussion of the space fluid science experiment project, providing useful references for future long-term in-orbit scientific research using cargo spacecraft.
Functional Hypergraphs of Stock Markets
Authors: David Jerry Jones , Sabhahit Narayan G. , Stramaglia Sebastiano , Matteo T. Di , Boccaletti Stefano , Jalan Sarika
Published: October 2024

In stock markets, nonlinear interdependencies between various companies result in nontrivial time-varying patterns in stock prices. A network representation of these interdependencies has been successful in identifying and understanding hidden signals before major events like stock market crashes. However, these studies have revolved around the assumption that correlations are mediated in a pairwise manner, whereas, in a system as intricate as this, the interactions need not be limited to pairwise only. Here, we introduce a general methodology using information-theoretic tools to construct a higher-order representation of the stock market data, which we call functional hypergraphs. This framework enables us to examine stock market events by analyzing the following functional hypergraph quantities: Forman–Ricci curvature, von Neumann entropy, and eigenvector centrality. We compare the corresponding quantities of networks and hypergraphs to analyze the evolution of both structures and observe features like robustness towards events like crashes during the course of a time period.
Hunting for Bileptons at Hadron Colliders
Authors: Corcella Gennaro
Published: October 2024

I review possible signals at hadron colliders of bileptons, namely doubly charged vectors or scalars with lepton number L=±2, as predicted by a 331 model, based on a SU(3)c×SU(3)L×U(1)X symmetry. In particular, I account for a version of the 331 model wherein the embedding of the hypercharge is obtained with the addition of three exotic quarks and vector bileptons. Furthermore, a sextet of SU(3)L, necessary to provide masses to leptons, yields an extra scalar sector, including a doubly charged Higgs, i.e., scalar bileptons. As bileptons are mostly produced in pairs at hadron colliders, their main signal is provided by two same-sign lepton pairs at high invariant mass. Nevertheless, they can also decay according to non-leptonic modes, such as a TeV-scale heavy quark, charged 4/3 or 5/3, plus a Standard Model quark. I explore both leptonic and non-leptonic decays and the sensitivity to the processes of the present and future hadron colliders.
Evaluating Microgrid Investments: Introducing the MPIR Index for Economic and Environmental Synergy
Authors: Papadopoulos Agis M. , Symeonidou Maria
Published: October 2024

In view of the increasing environmental challenges and the growing demand for sustainable energy solutions, the optimization of microgrid systems with regard to economic efficiency and environmental compatibility is becoming ever more important. This paper presents the Microgrid Performance and Investment Rating (MPIR) index, a novel assessment framework developed to link economic and environmental objectives within microgrid configurations. The MPIR index evaluates microgrid configurations based on five critical dimensions: financial viability, sustainability, regional renewable integration readiness, energy demand, and community engagement, facilitating comprehensive and balanced decision making. The current cases focus on the area of Greece; however, the model can have a wider application. Developed using a two-target optimization model, this index integrates various energy sources—including photovoltaics, micro-wind turbines, and different types of batteries—with advanced energy management strategies to assess and improve microgrid performance. This paper presents case studies in which the MPIR index is applied to different microgrid scenarios. It demonstrates its effectiveness in identifying optimal configurations that reduce the carbon footprint while maximizing economic returns. The MPIR index provides a quantifiable, scalable tool for stakeholders, not only advancing the field of microgrid optimization, but also aligning with global sustainability goals and promoting the transition to a more resilient and sustainable energy future.
Relevance-Based Reconstruction Using an Empirical Mode Decomposition Informer for Lithium-Ion Battery Surface-Temperature Prediction
Authors: Li Chao , Kong Yigang , Wang Changjiang , Wang Xueliang , Wang Min , Wang Yulong
Published: October 2024

Accurate monitoring of lithium-ion battery temperature is essential to ensure these batteries’ efficient and safe operation. This paper proposes a relevance-based reconstruction-oriented EMD-Informer machine learning model, which combines empirical mode decomposition (EMD) and the Informer framework to estimate the surface temperature of 18,650 lithium-ion batteries during charging and discharging processes under complex operating conditions. Initially, based on 9000 data points from the U.S. NASA Prognostics Center of Excellence’s random battery-usage dataset, where each data point includes three features: temperature, voltage, and current, EMD is used to decompose the temperature data into intrinsic mode functions (IMFs). Subsequently, the IMFs are reconstructed into low-, medium-, and high-correlation components based on their correlation with the original data. These components, along with voltage and current data, are fed into sub-models. Finally, the model captures the long-term dependencies among temperature, voltage, and current. The experimental results show that, in single-step prediction, the mean squared error, mean absolute error, and maximum absolute error of the model’s predictions are 0.00095, 0.02114, and 0.32164 °C; these metrics indicate the accurate prediction of the surface temperature of lithium-ion batteries. In multi-step predictions, when the prediction horizon is set to 12 steps, the model achieves a hit rate of 93.57% where the maximum absolute error is within 0.5 °C; under these conditions, the model combines high predictive accuracy with a broad predictive range, which is conducive to the effective prevention of thermal runaway in lithium-ion batteries.
Heat Transfer Performance Factors in a Vertical Ground Heat Exchanger for a Geothermal Heat Pump System
Authors: Salhein Khaled , Kobus C. J. , Zohdy Mohamed , Annekaa Ahmed M. , Alhawsawi Edrees Yahya , Salheen Sabriya Alghennai
Published: October 2024

Ground heat pump systems (GHPSs) are esteemed for their high efficiency within renewable energy technologies, providing effective solutions for heating and cooling requirements. These GHPSs operate by utilizing the relatively constant temperature of the Earth’s subsurface as a thermal source or sink. This feature allows them to perform greater energy transfer than traditional heating and cooling systems (i.e., heating, ventilation, and air conditioning (HVAC)). The GHPSs represent a sustainable and cost-effective temperature-regulating solution in diverse applications. The ground heat exchanger (GHE) technology is well known, with extensive research and development conducted in recent decades significantly advancing its applications. Improving GHE performance factors is vital for enhancing heat transfer efficiency and overall GHPS performance. Therefore, this paper provides a comprehensive review of research on various factors affecting GHE performance, such as soil thermal properties, backfill material properties, borehole depth, spacing, U-tube pipe properties, and heat carrier fluid type and velocity. It also discusses their impact on heat transfer efficiency and proposes optimal solutions for improving GHE performance.
Solar Chimney Operation Variant
Authors: Gortych Marta , Lipnicki Zygmunt , Małolepszy Tomasz , Grabas Piotr
Published: October 2024

This paper presents a solar chimney that acts as a heat accumulator. It is based on its alternating charging (melting of the phase change material—PCM) and discharging (solidification), which helps to save energy and ensures stable operation of the solar chimney. In this paper, special attention has been paid to the heat dissipation process (solidification of the PCM). The theoretical model of solidification has been solved in an original way. This paper presents a new simple theoretical model for the solidification of the PCM on a flat plate and presents the results of numerical tests. The theoretical model presents a method for determining the heat transfer coefficient at the solidification front of the PCM. In addition, the heat transfer coefficient from the flowing air to the outer surface of the solidifying front plate was determined experimentally in an original way. The heat transfer coefficient values resulting from the experiments may be employed in order to calculate the heat transfer coefficient for air flowing through the slot of the collector in the solar chimney. The calculated value of the heat transfer coefficient was 18.55 W/m²K.
Analysis of Spatial and Temporal Trends of Vegetation Cover Evolution and Its Driving Forces from 2000 to 2020—A Case Study of the WuShen Counties in the Maowusu Sandland
Authors: Zhao Zeyu , Liu Xiaomin , Liu Tingxi , Wu Yingjie , Wang Wenjuan , Tian Yun , Fu Laichen
Published: October 2024

The WuShen counties in the hinterland of the Maowusu Sandland are located in the “ecological stress zone” of the forest–steppe desert, with low vegetation cover, a strong ecosystem sensitivity, and poor stability under the influence of human activities. Therefore, it is important to study and analyze the changes in vegetation growth in this region for the purpose of objectively evaluating the effectiveness of desertification control in China’s agricultural and pastoral intertwined zones, and formulating corresponding measures in a timely manner. In this paper, the spatial and temporal variations in the vegetation NDVI in the WuShen counties of the Maowusu Sandland and their response relationships with driving factors were investigated by using a trend test, center of gravity transfer model, partial correlation calculation, and residual analysis, and by using the MOD13A3 vegetation NDVI time series data from 2000 to 2020, as well as the precipitation, temperature, and potential evapotranspiration data from the same period. The results showed the following: ① The regional vegetation NDVI did not fluctuate significantly with latitude and longitude, and the NDVI varied between 0.227 and 0.375 over the 21-year period, with a mean increase of 0.13 for the region as a whole and an increase of 0.61 for the region of greatest change. Of the area, 86.83% experienced a highly significant increase, and the trend in increase around rivers and towns was higher than that in the northwestern inland flow area, with the overall performance of “low in the west and high in the east”. ② Only 2.07% of the vegetation NDVI center of gravity did not shift, and the response with climate factors was mainly characterized by having consistent or opposite center of gravity changes with precipitation and potential evapotranspiration. ③ Human activities have been the dominant factor in the vegetation NDVI change, with 75.89 percent of the area positively impacted by human activities, and human activities in the southwest inhibiting the improvement of vegetation in the area. The impact of human activities on the unchanged land type area is increasing, most obviously in the farmland area, and the impact of human activities on the changed land type area is gradually decreasing in the area where the farmland becomes impervious. The vegetation in the area above 1300 m above sea level is degraded by the environment and human activities. The research results can provide scientific support for the implementation of ecological fine management and the formulation of corresponding ecological restoration and desertification control measures in the Maowusu Sandland. At the same time, it is expected to serve as a baseline for other studies on the evolution of vegetation in agro-pastoral zones.
Bioprospecting of Metschnikowia pulcherrima Strains, Isolated from a Vineyard Ecosystem, as Novel Starter Cultures for Craft Beer Production
Authors: Karaulli Julian , Xhaferaj Nertil , Coppola Francesca , Testa Bruno , Letizia Francesco , Kyçyk Onejda , Kongoli Renata , Ruci Mamica , Lamçe Fatbardha , Sulaj Kapllan , Iorizzo Massimo
Published: October 2024

Several studies in recent years have shown that the use of non-Saccharomyces yeasts, used both in single and in mixed fermentations with Saccharomyces cerevisiae, can help produce craft beers with distinctive compositional characteristics. The aim of this study was to evaluate the suitability of three Metschnikowia pulcherrima strains, isolated from Albanian vineyards, for use as starters in the brewing process. Because of its specific enzymatic activities (protease, β-glucosidase, and β-lyase) and its low production of hydrogen sulfide, M. pulcherrima 62 was selected as a starter culture for the production of craft beer. Specifically, the suitability of this yeast for use in sequential inoculation with S. cerevisiae S0-4 for the production of an American IPA-style beer and the main volatile compounds produced during fermentation were evaluated. The results show significant differences in the glycerol, isoamyl alcohol, and isoamyl acetate contents in beer obtained by sequential inoculum of M. pulcherrima 62 with S. cerevisiae S0-4 compared to beer obtained using S. cerevisiae S0-4 as a single starter. Therefore, these preliminary data support the candidacy of M. pulcherrima 62 as a new starter in the brewing process.
Amelogenin-Based Molecular Methods for Sexual Dimorphism Identification: Protocol of a Scoping Review
Authors: Lopes Cardoso Inês , Moreira Maria Teresa , Dupuis Clarisse , Castro Filipe , Guimarães Maria Inês
Published: October 2024

Forensic dentistry and sexual dimorphism are distinct concepts. Still, they are related due to the usefulness that the first may have in the second, and this review focuses on them. A scoping review will be performed according to the Joanna Briggs Institute’s methodology. Scientific databases and grey literature will be used, and the following keywords will be applied: amelogenin, analyses, sex determination, and human identification. This scoping review will include in vitro studies concerning the goal of this review. This scoping review will deepen our knowledge concerning using teeth and amelogenin genes in sex identification in a forensic context. According to the available data, it will help implement guidelines for human remains identification. This protocol was registered with the Open Science Framework.
Some Mathematical Examples of Emergent Intuitive Local Time Flow
Authors: Esquível Manuel L. , Krasii Nadezhda P. , Didier Philippe L.
Published: October 2024

After reviewing important historical and present day ideas about the concept of time, we develop some instances of mathematical examples where, from the interaction of concepts that model interactions of things in the observable world, time flow emerges in an intuitive and local interpretation. We present several instances of emergence of time flow in mathematical contexts, to wit, by specific parametrisation of deterministic and stochastic curves or of geodesics in Riemann manifolds.
Chromosome 15q11-q13 Duplication Syndrome: A Review of the Literature and 14 New Cases
Authors: Bisba Maria , Malamaki Christina , Constantoulakis Pantelis , Vittas Spiros
Published: October 2024

The 15q11.2q13 chromosomal region is particularly susceptible to chromosomal rearrangements due to low-copy repeats (LCRs) located inside this area. Specific breakpoints (BP1-BP5) that lead to deletions and duplications of variable size have been identified. Additionally, this specific region contains several imprinted genes, giving rise to complex syndromes (Prader–Willi, Angelman and 15q11-q13 duplication syndromes). 15q11.2-q13 duplication syndrome has been associated with neurodevelopmental disorders (hypotonia, developmental delay, speech delay and seizures) and ASD but is characterized by variable expressivity and reduced penetrance, features that make genetic counseling a complex procedure especially in prenatal cases. In the present study, a total of 14 pre- and postnatal cases were diagnosed as 15q11.2q13 duplication carriers using Affymetrix CytoScan 750 K array-CGH, and our analysis combined these with 120 cases existing in the literature. The inheritance pattern of the cases of this study is unknown, but as a review of the literature revealed, 62.96% of the affected carriers inherited the duplicated area from their mother. The combined results of this analysis (the present study and the literature) show that in the majority of the cases, the phenotype is a compound phenotype, with clinical characteristics that include ASD, intellectual disability, developmental delay and an absence of speech. The aim of this paper is to deliver new possibilities to genetic counseling that can be provided in prenatal and postnatal cases as the phenotype of 15q11.2q13 microduplication carriers cannot be fully predicted; so, clinical diagnoses should be a combination of molecular findings and clinical manifestations that are present.
High Impedance Active Probe for High Voltages
Authors: Boillat Marc-Aurèle , Hauser Peter C.
Published: October 2024

The probe was designed for the measurement of DC voltages of up to 30 kV from high impedance sources. It is based on a resistive divider with a total resistance of 200 GΩ and a step-down factor of 10’000. In order to allow the measurement of the stepped down voltage with a conventional multimeter without loading, the signal was buffered with an operational amplifier. The device was calibrated against a commercial probe using a low impedance high voltage source. A linear relationship was obtained for a high impedance resistive ladder for voltages between 3 and 30 kV, with a coefficient of determination (R2) of 0.9999. The low-cost device (ca. US $200) fills an application niche not addressed by commercial products.
Functional Analysis of Cytochrome b5 in Regulating Anthocyanin Biosynthesis in Malus domestica
Authors: Zhang Fu-Jun , Ma Ning , Li Hao-Jian , Li Lian-Zhen , Zhang De-En , Zhang Zhen-Lu , You Chun-Xiang , Lu Xiao-Yan
Published: October 2024

Cytochrome b5 (CB5), a small heme-binding protein, plays an important role in plant biotic and abiotic stress. Anthocyanin is a critical determinant for fruit coloration, however, whether CB5 is involved in regulating anthocyanin biosynthesis has not yet been investigated in apple fruit (Malus domestica). In this study, we determined that MdCYB5, an apple CB5 gene, was a positive regulator for anthocyanin biosynthesis in apple fruit. We first found that MdCYB5 showed a high sequence and structural similarity with Arabidopsis cytochrome b5 isoform E (CB5E) at the protein level. Quantitative reverse transcription PCR (qRT-PCR) analysis showed that MdCYB5 responds to light signals. Subcellular localization showed that MdCYB5 is localized to the cytoplasmin inthe epidermal cells of Nicotiana benthamiana leaves. Further investigation revealed that overexpressing MdCYB5 promoted anthocyanin biosynthesis in both apple calli and tissue-cultured apple seedlings. Furthermore, results of transient expression assay showed that overexpressing MdCYB5 promoted anthocyanin accumulation and fruit coloration in apple fruit. Taken together, this study suggests that MdCYB5 has a positive regulatory effect on anthocyanin biosynthesis in apple fruit.
Living and Researching the COVID-19 Pandemic: Autoethnographic Reflections from a Co-Research Team of Older People and Academics
Authors: McCabe Louise , Brown Tamara , Anderson Roy , Chrystall Liz , Curry David , Fairclough Margot , Ritchie Christine , Scrutton Pat , Smith Ann , Douglas Elaine
Published: October 2024

This article describes and reflects upon the work of a co-research team on the Healthy Ageing in Scotland (HAGIS) ‘COVID-19 Impact and Recovery’ study (January 2021 to November 2022). The co-research team (seven older adults and three academics) was constituted near the start of this project; the team contributed to the development of recruitment materials and research tools and undertook qualitative research and analysis with older adults living across Scotland. This article provides a collaborative autoethnography about the activities undertaken by the team, the impact of the co-research process on the individuals involved, and the research findings and reflects the realities of co-research during the COVID-19 pandemic. Team members describe benefits, including increased confidence, new skills, and social connections, and reflect on the increased validity of the findings through their close involvement in the co-creation of knowledge. The process of team building and the adoption of an ‘ethics of care’ in our practice underpinned the success of this project and the sustainability of the group during and after the challenging circumstances of the pandemic.
An Algorithm for Simplifying 3D Building Models with Consideration for Detailed Features and Topological Structure
Authors: Li Zhenglin , Zhao Zhanjie , Gao Wujun , Jiao Li
Published: October 2024

To tackle problems such as the destruction of topological structures and the loss of detailed features in the simplification of 3D building models, we propose a 3D building model simplification algorithm that considers detailed features and topological structures. Based on the edge collapse algorithm, the method defines the region formed by the first-order neighboring triangles of the endpoints of the edge to be collapsed as the simplification unit. It incorporates the centroid displacement of the simplification unit, significance level, and approximate curvature of the edge as influencing factors for the collapse cost to control the edge collapse sequence and preserve model details. Additionally, considering the unique properties of 3D building models, boundary edge detection and face overlay are added as constraints to maintain the model’s topological structure. The experimental results show that the algorithm is superior to the classic QEM algorithm in terms of preserving the topological structure and detailed features of the model. Compared to the QEM algorithm and the other two comparison algorithms selected in this paper, the simplified model resulting from this algorithm exhibit a reduction in Hausdorff distance, mean error, and mean square error to varying degrees. Moreover, the advantages of this algorithm become more pronounced as the simplification rate increases. The research findings can be applied to the simplification of 3D building models.
Antinociceptive Behavior, Glutamine/Glutamate, and Neopterin in Early-Stage Streptozotocin-Induced Diabetic Neuropathy in Liraglutide-Treated Mice under a Standard or Enriched Environment
Authors: Gateva Pavlina , Hristov Milen , Ivanova Natasha , Vasileva Debora , Ivanova Alexandrina , Sabit Zafer , Bogdanov Todor , Apostolova Sonia , Tzoneva Rumiana
Published: October 2024

Diabetic neuropathy (DN) is a common complication of long-lasting type 1 and type 2 diabetes, with no curative treatment available. Here, we tested the effect of the incretin mimetic liraglutide in DN in mice with early-stage type 1 diabetes bred in a standard laboratory or enriched environment. With a single i.p. injection of streptozotocin 150 mg/kg, we induced murine diabetes. Liraglutide (0.4 mg/kg once daily, i.p. for ten days since the eighth post-streptozotocin day) failed to decrease the glycemia in the diabetic mice; however, it alleviated their antinociceptive behavior, as tested with formalin. The second phase of the formalin test had significantly lower results in liraglutide-treated mice reared in the enriched environment vs. liraglutide-treated mice under standard conditions [2.00 (0.00–11.00) vs. 29.00 (2.25–41.50) s, p = 0.016]. Liraglutide treatment, however, decreased the threshold of reactivity in the von Fray test. A significantly higher neopterin level was demonstrated in the diabetic control group compared to treatment-naïve controls and the liraglutide-treated diabetic mice (p < 0.001). The glutamine/glutamate ratio in both liraglutide-treated groups, either reared under standard conditions (p = 0.003) or an enriched environment (p = 0.002), was significantly higher than in the diabetic controls. This study demonstrates an early liraglutide effect on pain sensation in two streptozotocin-induced diabetes mouse models by reducing some inflammatory and oxidative stress parameters.
Special Issue: “Molecular Dynamics Simulations and Structural Analysis of Protein Domains”
Authors: de Brevern Alexandre G.
Published: October 2024

The 3D protein structure is the basis for all their biological functions [...]
Hepatokines and MASLD: The GLP1-Ras-FGF21-Fetuin-A Crosstalk as a Therapeutic Target
Authors: Milani Ilaria , Codini Michela , Guarisco Gloria , Chinucci Marianna , Gaita Chiara , Leonetti Frida , Capoccia Danila
Published: October 2024

The introduction of the term “Metabolic Steatotic Liver Disease” (MASLD) underscores the critical role of metabolic dysfunction in the development and progression of chronic liver disease and emphasizes the need for strategies that address both liver disease and its metabolic comorbidities. In recent years, a liver-focused perspective has revealed that altered endocrine function of the fatty liver is a key contributor to the metabolic dysregulation observed in MASLD. Due to its secretory capacity, the liver’s increased production of proteins known as “hepatokines” has been linked to the development of insulin resistance, explaining why MASLD often precedes dysfunction in other organs and ultimately contributes to systemic metabolic disease. Among these hepatokines, fibroblast growth factor 21 (FGF21) and fetuin-A play central roles in regulating the metabolic abnormalities associated with MASLD, explaining why their dysregulated secretion in response to metabolic stress has been implicated in the metabolic abnormalities of MASLD. This review postulates why their modulation by GLP1-Ras may mediate the beneficial metabolic effects of these drugs, which have increased attention to their emerging role as pharmacotherapy for MASLD. By discussing the crosstalk between GLP1-Ras-FGF21-fetuin-A, this review hypothesizes that the possible modulation of fetuin-A by the novel GLP1-FGF21 dual agonist pharmacotherapy may contribute to the management of metabolic and liver diseases. Although research is needed to go into the details of this crosstalk, this topic may help researchers explore the mechanisms by which this type of pharmacotherapy may manage the metabolic dysfunction of MASLD.
An Enhanced Interaction of Graft and Exogenous SA on Photosynthesis, Phytohormone, and Transcriptome Analysis in Tomato under Salinity Stress
Authors: Miao Chen , Zhang Yongxue , Cui Jiawei , Zhang Hongmei , Wang Hong , Jin Haijun , Lu Panling , He Lizhong , Zhou Qiang , Yu Jizhu , Ding Xiaotao
Published: October 2024

Salt stress can adversely affect global agricultural productivity, necessitating innovative strategies to mitigate its adverse effects on plant growth and yield. This study investigated the effects of exogenous salicylic acid (SA), grafting (G), and their combined application (GSA) on various parameters in tomato plants subjected to salt stress. The analysis focused on growth characteristics, photosynthesis, osmotic stress substances, antioxidant enzyme activity, plant hormones, ion content, and transcriptome profiles. Salt stress severely inhibits the growth of tomato seedlings. However, SA, G, and GSA improved the plant height by 22.5%, 26.5%, and 40.2%; the stem diameter by 11.0%, 26.0%, and 23.7%; the shoot fresh weight by 76.3%, 113.2%, and 247.4%; the root fresh weight by 150.9%, 238.6%, and 286.0%; the shoot dry weight by 53.5%, 65.1%, and 162.8%; the root dry weight by 150.0%, 150.0%, and 166.7%, and photosynthesis by 4.0%, 16.3%, and 32.7%, with GSA presenting the most pronounced positive effect. Regarding the osmotic stress substances, the proline content increased significantly by more than 259.2% in all treatments, with the highest levels in GSA. Under salt stress, the tomato seedlings accumulated high Na+ levels; the SA, G, and GSA treatments enhanced the K+ and Ca2+ absorption while reducing the Na+ and Al3+ levels, thereby alleviating the ion toxicity. The transcriptome analysis indicated that SA, G, and GSA influenced tomato growth under salt stress by regulating specific signaling pathways, including the phytohormone and MAPK pathways, which were characterized by increased endogenous SA and decreased ABA content. The combined application of grafting and exogenous SA could be a promising strategy for enhancing plant tolerance to salt stress, offering potential solutions for sustainable agriculture in saline environments.
Compositional Optimization of Sputtered SnO2/ZnO Films for High Coloration Efficiency
Authors: Lábadi Zoltán , Ismaeel Noor Taha , Petrik Péter , Fried Miklós
Published: October 2024

We performed an electrochromic investigation to optimize the composition of reactive magnetron-sputtered mixed layers of zinc oxide and tin oxide (ZnO-SnO2). Deposition experiments were conducted as a combinatorial material synthesis approach. The binary system for the samples of SnO2-ZnO represented the full composition range. The coloration efficiency (CE) was determined for the mixed oxide films with the simultaneous measurement of layer transmittance, in a conventional three-electrode configuration, and an electric current was applied by using organic propylene carbonate electrolyte cells. The optical parameters and composition were measured and mapped by using spectroscopic ellipsometry (SE). Scanning Electron Microscopy (SEM) and Energy-Dispersive X-ray Spectroscopy (EDS) measurements were carried out to check the SE results, for (TiO2-SnO2). Pure metal targets were placed separately from each other, and the indium–tin-oxide (ITO)-covered glass samples and Si-probes on a glass holder were moved under the two separated targets (Zn and Sn) in a reactive argon–oxygen (Ar-O2) gas mixture. This combinatorial process ensured that all the compositions (from 0 to 100%) were achieved in the same sputtering chamber after one sputtering preparation cycle. The CE data evaluated from the electro-optical measurements plotted against the composition displayed a characteristic maximum at around 29% ZnO. The accuracy of our combinatorial approach was 5%.
In Silico Design of miniACE2 Decoys with In Vitro Enhanced Neutralization Activity against SARS-CoV-2, Encompassing Omicron Subvariants
Authors: Arévalo-Romero Jenny Andrea , López-Cantillo Gina , Moreno-Jiménez Sara , Marcos-Alcalde Íñigo , Ros-Pardo David , Camacho Bernardo Armando , Gómez-Puertas Paulino , Ramírez-Segura Cesar A.
Published: October 2024

The COVID-19 pandemic has overwhelmed healthcare systems and triggered global economic downturns. While vaccines have reduced the lethality rate of SARS-CoV-2 to 0.9% as of October 2024, the continuous evolution of variants remains a significant public health challenge. Next-generation medical therapies offer hope in addressing this threat, especially for immunocompromised individuals who experience prolonged infections and severe illnesses, contributing to viral evolution. These cases increase the risk of new variants emerging. This study explores miniACE2 decoys as a novel strategy to counteract SARS-CoV-2 variants. Using in silico design and molecular dynamics, blocking proteins (BPs) were developed with stronger binding affinity for the receptor-binding domain of multiple variants than naturally soluble human ACE2. The BPs were expressed in E. coli and tested in vitro, showing promising neutralizing effects. Notably, miniACE2 BP9 exhibited an average IC50 of 4.9 µg/mL across several variants, including the Wuhan strain, Mu, Omicron BA.1, and BA.2 This low IC50 demonstrates the potent neutralizing ability of BP9, indicating its efficacy at low concentrations.Based on these findings, BP9 has emerged as a promising therapeutic candidate for combating SARS-CoV-2 and its evolving variants, thereby positioning it as a potential emergency biopharmaceutical.
TNFα-Related Chondrocyte Inflammation Models: A Systematic Review
Authors: Wang Su , Kurth Sarah , Burger Christof , Wirtz Dieter C. , Schildberg Frank A. , Ossendorff Robert
Published: October 2024

Tumor necrosis factor alpha (TNFα), as a key pro-inflammatory cytokine, plays a central role in joint diseases. In recent years, numerous models of TNFα-induced cartilage inflammation have been developed. However, due to the significant differences between these models and the lack of consensus in their construction, it becomes difficult to compare the results of different studies. Therefore, we summarized and compared these models based on important parameters for model construction, such as cell source, cytokine concentration, stimulation time, mechanical stimulation, and more. We attempted to analyze the advantages and disadvantages of each model and provide a compilation of the analytical methods used in previous studies. Currently, TNFα chondrocyte inflammation models can be categorized into four main types: monolayer-based, construct-based, explant-based TNFα chondrocyte inflammation models, and miscellaneous TNFα chondrocyte inflammation models. The most commonly used models were the monolayer-based TNFα chondrocyte inflammation models (42.86% of cases), with 10 ng/mL TNFα being the most frequently used concentration. The most frequently used chondrocyte cell passage is passage 1 (50%). Human tissues were most frequently used in experiments (51.43%). Only five articles included models with mechanical stimulations. We observed variations in design conditions between different models. This systematic review provides the essential experimental characteristics of the available chondrocyte inflammation models with TNFα, and it provides a platform for better comparison between existing and new studies in this field. It is essential to perform further experiments to standardize each model and to find the most appropriate experimental parameters.
Optimization of the Treatment of Squamous Cell Carcinoma Cells by Combining Photodynamic Therapy with Cold Atmospheric Plasma
Authors: Karrer Sigrid , Unger Petra , Spindler Nina , Szeimies Rolf-Markus , Bosserhoff Anja Katrin , Berneburg Mark , Arndt Stephanie
Published: October 2024

Actinic keratosis (AK) is characterized by a reddish or occasionally skin-toned rough patch on sun-damaged skin, and it is regarded as a precursor to squamous cell carcinoma (SCC). Photodynamic therapy (PDT), utilizing 5-aminolevulinic acid (ALA) along with red light, is a recognized treatment option for AK that is limited by the penetration depth of light and the distribution of the photosensitizer into the skin. Cold atmospheric plasma (CAP) is a partially ionized gas with permeability-enhancing and anti-cancer properties. This study analyzed, in vitro, whether a combined treatment of CAP and ALA-PDT may improve the efficacy of the treatment. In addition, the effect of the application sequence of ALA and CAP was investigated using in vitro assays and the molecular characterization of human oral SCC cell lines (SCC-9, SCC-15, SCC-111), human cutaneous SCC cell lines (SCL-1, SCL-2, A431), and normal human epidermal keratinocytes (HEKn). The anti-tumor effect was determined by migration, invasion, and apoptosis assays and supported the improved efficacy of ALA-PDT in combination with CAP. However, the application sequence ALA-CAP–red light seems to be more efficacious than CAP-ALA–red light, which is probably due to increased intracellular ROS levels when ALA is applied first, followed by CAP and red light treatment. Furthermore, the expression of apoptosis- and senescence-related molecules (caspase-3, -6, -9, p16INK4a, p21CIP1) was increased, and different genes of the junctional network (ZO-1, CX31, CLDN1, CTNNB1) were induced after the combined treatment of CAP plus ALA-PDT. HEKn, however, were much less affected than SCC cells. Overall, the results show that CAP may improve the anti-tumor effects of conventional ALA-PDT on SCC cells. Whether this combined application is successful in treating AK in vivo has to be carefully examined in follow-up studies.
Preparation and Characterization of Novel Poly(Lactic Acid) Composites Reinforced with “Latxa” Sheep Wool Fibers: The Effect of Peroxide Surface Treatments and Fiber Content
Authors: Arbelaiz Aitor , Yurramendi Telmo , Larruscain Ander , Arrizabalaga Ane , Eceiza Arantxa , Peña-Rodriguez Cristina
Published: October 2024

“Latxa” sheep wool is rough, and it is not used in the textile industry because the fiber diameter is high compared with other wool fibers. Nowadays, this wool is considered as disposal and, with the aim to give it value, new uses must be explored. In the current work, the “Latxa” sheep wool fiber was evaluated as poly(lactic acid) (PLA) polymer reinforcement. With the objective to optimize fiber/matrix adhesion, fibers were surface modified with peroxide. Oxidation treatment with peroxide led to chemical modifications of the wool fibers that improved the fiber/PLA adhesion, but the strength values achieved for the composites were lower compared to the neat PLA ones. The mechanical properties obtained in the current work were compared with the literature data of the PLA composites reinforced with vegetable fibers. The wool fibers showed inferior mechanical properties compared to the vegetable fiber counterparts. However, the preliminary results indicated that the incorporation of wool fibers to PLA reduced the flammability of composites.
Influence of Conductive Filler Types on the Ratio of Reflection and Absorption Properties in Cement-Based EMI Shielding Composites
Authors: Jang Daeik , Park Jihoon , Jang Woosuk , Bang Jinho , Kim G. M. , Choi Jaesuk , Seo Joonho , Yang Beomjoo
Published: October 2024

The growing importance of electromagnetic interference (EMI) shielding composites in civil engineering has garnered increasing attention. Conductive cement-based composites, incorporating various conductive fillers, such as carbon nanotubes (CNTs), carbon fibers (CFs), and graphene nanoplatelets (GNPs), provide effective solutions due to their high electrical conductivity. While previous studies have primarily focused on improving the overall shielding effectiveness, this research emphasizes balancing the reflection and absorption properties. The experimental results demonstrate an EMI shielding performance exceeding 50 dB, revealing that filler size (nano, micro, or macro) and shape (platelet or fiber) significantly influence both reflection and absorption characteristics. Based on a comprehensive evaluation of the shielding properties, this study highlights the need to consider factors such as reflection versus absorption losses and filler shape or type when optimizing filler content to develop effective cement-based EMI shielding composites.
Cystic Angiomyofibroblastoma of the Uterus Mimicking Ovarian Cancer
Authors: Jo Jae Yoon , An Hyo Jung , Jo In Ae , Shin Jeong Kyu , Choi Won Jun , Baek Jong Chul
Published: October 2024

Angiomyofibroblastoma (AMFB) is an exceedingly rare mesenchymal tumor of the lower genital tract. AMFB primarily affects the pelviperineal region, especially the vulvar in premenopausal women. Typically, AMFB is a benign disease and does not have the potential for metastasis or recurrence, requiring complete surgical excision. Its accurate differentiation from aggressive angiomyxoma is critical due to varying prognoses. A 51-year-old woman, diagnosed with mucinous carcinoma of the breast, presented with a 12 cm abdominopelvic mass identified during breast cancer staging. Imaging suggested an ovarian origin; however, surgical exploration revealed a stalk-attached cystic mass in the anterior body of the uterus. Histopathology confirmed AMFB. Immunohistochemical analysis showed positivity for estrogen and progesterone receptors and smooth muscle actin. The patient continued breast cancer treatment postoperatively without pelvic mass recurrence or complications for a postoperative follow-up period of one year. This case highlights AMFB’s potential uterine body origin, expending known tumor sites and complicating diagnosis due to overlapping features with other mesenchymal tumors. Accurate diagnosis using immunohistochemical markers and pathological features is essential to avoid unnecessary aggressive treatments. The uterine location in this case suggests a possible shared pathogenesis with uterine myomas, warranting further research into their connection. Reporting the first case of AMFB originating in the uterine body enhances understanding of this rare condition and underscores the importance of clinical awareness and precise diagnostic strategies to guide management and improve outcomes.
Sampling Confined Fission Tracks for Constraining Geological Thermal Histories
Authors: Jonckheere Raymond , Aslanian Carolin , Fu Hongyang , Trilsch Florian
Published: October 2024

Fission-track modeling rests on etching, counting and measuring the lattice damage trails from uranium fission. The tools for interpreting fission-track data are advanced but the results are never better than the data. Confined-track samples must be an adequate size for statistical analysis, representative of the track population and consistent with the model assumptions and with the calibration data. Geometrical and measurement biases are understood and can be dealt with up to a point. However, the interrelated issues of etching protocol and track selection are more difficult to untangle. Our investigation favors a two-step protocol. The duration of the first step is inversely proportional to the apatite etch rate so that different apatites etch to the same Dpar. A long immersion reveals many more confined tracks, terminated by basal and prism faces. This allows consistent length measurements and permits orienting each track relative to the c-axis. Long immersion times combined with deep ion irradiation reveal confined tracks deep inside the grains. Provided it is long enough, the precise immersion time is not important if the effective etch times of the selected tracks are calculated from their measured widths. Then, whether the sample is mono- or multi-compositional, we can, post hoc, select tracks with the desired properties. The second part of the protocol has to do with the fact that fossil tracks in geological samples appear to be under-etched compared to induced tracks etched under the same conditions. This should be assumed if the semi-axes of a fitted ellipse plot above the induced-track line. In that case, an additional etch can increase the track lengths to a point where they are consistent with the model based on lab-annealing of induced tracks, a condition for valid thermal histories. Here too, it is possible to select a subset of tracks with effective etch times consistent with the model if the widths of confined tracks are measured along with their lengths and orientations.
The Effects of Collagen Peptides as a Dietary Supplement on Muscle Damage Recovery and Fatigue Responses: An Integrative Review
Authors: Inacio Pedro Augusto Querido , Gomes Yasmin Salgado Mussel , de Aguiar Ana Julia Nunes , Lopes-Martins Pedro Sardinha Leonardo , Aimbire Flávio , Leonardo Patrícia Sardinha , Sá Filho Alberto Souza , Lopes-Martins Rodrigo Alvaro B.
Published: October 2024

Background/objectives: The oral administration of hydrolyzed collagen peptides is a scientifically validated intervention for enhancing skeletal muscle health and performance. This integrative review consolidates the evidence supporting the use of low molecular weight collagen peptides (2000–3500 daltons) for their superior bioavailability and absorption. Our objective was to review the effects of collagen peptide or hydrolyzed collagen supplementation on muscle damage, recovery, and construction related to physical exercise. Methods: A bibliographic search was conducted in major English-language databases, including PubMed/Medline, using terms like “Peptides Collagen and Damage” and “collagen peptides AND Soreness Muscle”. This review followed PRISMA guidelines, with bias risk assessed via the PEDro scale. The inclusion criteria were (a) randomized clinical trials, (b) randomized studies in humans with a control or placebo group, (c) studies assessing muscle damage or delayed onset muscle soreness via physiological markers or strength performance tests, and (d) studies using hydrolyzed collagen or collagen peptides. Results: Initially, 752 articles were identified. After applying the inclusion and exclusion criteria, including duplicate removal, eight articles with 286 participants were included. Of these, 130 participants received collagen peptide supplementation, while 171 received a placebo or control. Conclusion: This integrative review supports the potential of collagen peptide supplementation to mitigate muscle stress from acute strenuous resistance training. However, due to the methodological heterogeneity among the studies, further clinical trials are needed to clarify the mechanisms underlying muscle improvement with collagen supplementation.
Relationship of Pain, Depression, Fatigue, and Sleep Problems with Functional Capacity, Balance, and Fear of Falling in Women with Fibromyalgia: Cross-Sectional Study
Authors: Denche-Zamorano Ángel , Pastor-Cisneros Raquel , Tomas-Carus Pablo , Adsuar-Sala José Carmelo , Salas-Gómez Diana , Parraca Jose Alberto
Published: October 2024

(1) Background: Fibromyalgia (FM) is a syndrome marked by chronic widespread pain, fatigue, sleep issues, and other symptoms. Interventions like physical exercise can potentially enhance physical function in individuals with FM. This study aimed to assess physical function, perceived physical fitness, balance confidence, and fear of falling in women with FM based on their levels of pain, depression, fatigue, and sleep problems. (2) Methods: Participants underwent a series of tests and questionnaires to evaluate physical and perceptual aspects. These included the Time Up and Go, Four Step Square Test, 6-Minute Walking Test, Maximum Handgrip Strength, Back Scratch, International Fitness Scale, Activities-Specific Balance Confidence Scale, and Fall Efficacy Scale—International. Participants were categorised by the severity of their pain, depression, fatigue, and sleep problems (mild, moderate, severe). A Kruskal–Wallis test assessed intergroup differences, while Spearman’s rho evaluated correlations between the study variables and symptom levels. (3) Results: Perceived physical condition varied significantly with symptom severity. Symptoms and sleep problems were notably linked to fear of falling, though no significant differences emerged in the physical tests. (4) Conclusions: In women with fibromyalgia, symptom severity was primarily related to perceptual and subjective aspects of physical condition and fall safety.