bluuebunny/crossref_metadata_2025 huggingface dataset:
Early access to science research opportunities: Growth within a geoscience summer research program for community college students
Authors: Okochi Christine , Gold Anne U. , Christensen Alicia , Batchelor Rebecca L.
Published: December 2023

Undergraduate research experiences benefit students by immersing them in the work of scientists and often result in increased interest and commitment to careers in the sciences. Expanding access to Research Experience for Undergraduate (REU) programs has the potential to engage more students in authentic research experiences earlier in their academic careers and grow and diversify the geoscience workforce. The Research Experience for Community College Students (RECCS) was one of the first National Science Foundation (NSF)-funded REU programs exclusively for 2-year college students. In this study, we describe findings from five years of the RECCS program and report on outcomes from 54 students. The study collected closed- and open-ended responses on post-program reflection surveys to analyze both student and mentor perspectives on their experience. Specifically, we focus on students’ self-reported growth in areas such as research skills, confidence in their ability to do research, and belonging in the field, as well as the mentors’ assessment of students’ work and areas of growth, and the impact of the program on students’ academic and career paths. In addition, RECCS alumni were surveyed annually to update data on their academic and career pursuits. Our data show that RECCS students learned scientific and professional skills throughout the program, developed a sense of identity as a scientist, and increased their interest in and excitement for graduate school after the program. Through this research experience, students gained confidence in their ability to “do” science and insight into whether this path is a good fit for them. This study contributes to an emerging body of data examining the impact of REU programs on community college students and encourages geoscience REU programs to welcome and support more community college students.
Research on the changes and predictions of the burden of type 2 diabetes mellitus in Pacific Island countries from 1990 to 2019
Authors: Li Yan , Zhang Hao , Jiang Yi
Published: December 2023

Aims

To assess the burden of type 2 diabetes in Pacific Island countries and predict future trends.

Methods

We analyzed and processed data using R and Excel software, performed Joinpoint 4.7.0 software analysis to investigate changing trends in disease burden, and used an autoregressive integrated moving average model to forecast future trends.

Results

Our study showed that from 1990 to 2019, the burden of type 2 diabetes in Pacific Island countries continues to increase, with the standardized incidence rate showing the most significant growth. Moreover, there were significant differences in the burden of type 2 diabetes between regions. In 2019, American Samoa had the highest standardized incidence rate, while Fiji had the highest standardized death rate and disability-adjusted life year rate. The standardized incidence rate peaked at ages 65–69 years, while the standardized death rate and disability-adjusted life year rate peaked at ages 95 years and 70–74 years respectively. Type 2 diabetes burden was higher among males than females. Based on our forecasting, from 2020 to 2030, the standardized incidence rate is expected to continue to rise, while the standardized death rate and disability-adjusted life year rate will slowly decline.

Conclusions

Our study highlights that the burden of type 2 diabetes in Pacific Island countries has been increasing from 1990 to 2019. Therefore, it is imperative to strengthen disease prevention and control measures in the region.
Evaluation of a longitudinal digital citizen science initiative to understand the impact of culture on Indigenous youth mental health: Findings from a quasi-experimental qualitative study
Authors: Walker Susannah , Kannan Prasanna , Bhawra Jasmin , Katapally Tarun Reddy
Published: December 2023

Background

Indigenous youth in settler nations are susceptible to poor mental health due to complex intergenerational systemic inequities. Research has shown benefits of cultural connectedness for improving mental health; however, there are few studies which have evaluated the impact of culturally relevant mental health interventions, particularly among Indigenous youth. The purpose of this study is to assess the impact of a culturally-responsive, land-based, active living initiative on the mental health of Indigenous youth.

Methods

This quasi-experimental qualitative study is part of Smart Indigenous Youth (SIY), a mixed-methods 5-year longitudinal digital citizen science initiative. SIY embeds culturally responsive, land-based active living programs into the curricula of high schools in rural Indigenous communities in the western Canadian province of Saskatchewan. In year-1 (Winter 2019), 76 Indigenous youth citizen scientists (13–18 years) from 2 schools participated in the study. At the beginning of the term, each school initiated separate 4-month land-based active living programs specific to their culture, community, geography, and language (Cree and Saulteaux). Before and after the term, focus groups were conducted with the 2 Youth Citizen Scientist Councils, which included students from both participating schools. This study includes data from focus groups of one participating school, with 11 youth citizen scientists (5 boys, 6 girls). Focus group data were transcribed and analyzed by two independent reviewers using Nvivo to identify themes and subthemes. Both reviewers discussed their thematic analysis to reach consensus about final findings.

Results

Baseline focus group analyses (before land-based programming) revealed themes demonstrating the importance of Indigenous culture, identity, history, and language. Youth emphasized the impact of loss of language and culture, the importance of being a helper, and the necessity of intergenerational knowledge transfer. Follow-up focus group analyses (post land-based programming) indicated that cultural school programming led to students expressing positive mental health benefits, increased interest in ceremonies, increased participation in physical activity, and greater knowledge of culture, identity, and ceremonial protocol.

Conclusions

This novel qualitative quasi-experimental study offers a window into the future of upstream interventions in partnership with Indigenous communities, where Indigenous youth can be engaged in real-time via their digital devices, while participating in culturally-sensitive, land-based school programming that promotes culture, identity, and mental health.
Evaluation of the osteogenic potential of demineralized and decellularized bovine bone granules following implantation in rat calvaria critical-size defect model
Authors: Al Qabbani Ali , Rani K. G. Aghila , AlKawas Sausan , Sheikh Abdul Hamid Suzina , Yap Abdullah Abdullah , Samsudin A. R. , Azlina Ahmad
Published: December 2023

The aim of this study was to compare the ability of demineralized (DMB) and decellularized (DCC) bovine bone granules to support bone regeneration in rat calvaria critical-size defects. DMB and DCC were prepared using a previously published method. The granule size used ranged between 500 and 750 μm. A total of forty-eight Sprague-Dawley rats were divided into two groups (n = 24). A pair of 5 mm diameter defects were created on the calvaria of the rats in the right and left parietal bone in both groups. Group A animals received DMB granules and Group B received DCC granules in the right parietal defect side while the left parietal untreated defect acted as sham surgery for both groups. Four animals per group were euthanized in a CO2 chamber at day 7, 14 and 21 post-surgery and the calvaria implantation site biopsy harvested was subjected to osteogenic gene expression analysis. Another four animals per group were euthanized at days 15, 30 and 60 post surgery and the calvaria implantation site biopsy harvested was subjected to histological, immunohistochemistry, RAMAN spectroscopy and Micro-CT analysis at the mentioned time points. Statistical analysis was conducted using t-tests and ANOVA. Histomorphometry showed significantly higher new bone formation in the DCC sites (p<0.05) compared to DMB. Both DMB and DCC implantation sites showed distinct staining for osteocalcin and osteopontin proteins compared to their respective sham sites. By day 21 after implantation, DCC sites demonstrated significantly elevated mRNA levels of osteonectin (p<0.001), osteopontin (p<0.001), osteocalcin (p<0.0001), ALP (p<0.01), and BMP-2 (p<0.001) compared to DMB. However, VEGF expression showed no significant differences at this time point between the two groups. Micro-CT analysis also showed enhanced defect closure and higher bone density in DCC implanted sites while RAMAN spectra demonstrated increased abundance of collagen and bone minerals, especially, PO43- ions than DMB. In conclusion, both DMB and DCC granules demonstrated favorable osteogenic potential in critical-sized defects, with DCC exhibited superior osteoconductive, osteoinductive and osteogenesis properties.
From birth to lying on- or under a supplemental heat source: How long does it take for half the litter to lie down and how long do they stay?
Authors: Stambuk Cassandra R. , Johnson Anna K. , Lane Karli J. , Stalder Kenneth J.
Published: December 2023

Piglets are susceptible to hypothermia because they lack hair and energy reserves, have a large surface area to body weight ratio, and have poor body thermostability. Different heat sources are used on farm, but it is not known how long it takes half a litter to locate it and lie down. The objectives of this study were to determine 1) how long it took for ≥ 50% of piglets to locate and lie on- or under the supplemental heat source for ≥ 5 min after the birth of the last-born pig and, 2) how long this cohort of piglets laid on- or under the heat source. A total of 12 sows were enrolled in the study (parity 1 = 4; 3 = 2; 4 = 2; 5 = 2; and 7 = 2). The stall containing one sow and her litter was the experimental unit. Two treatments were compared: 1) Baby Pig Heat Mat—Single 48 (MAT) and 2) Poly Heat Lamp (LAMP). Temperature was 32°C for both heat source treatments. Sow and litter video recordings occurred continually over a 24-h period on the day of farrowing. Two measures were determined 1) how long it took for ≥ 50% of piglets to locate and lie on- or under the supplemental heat source for ≥ 5 min after the birth of the last-born pig (h:min), and 2) how long this cohort of piglets laid on- or under the heat source (min:sec). Lying was defined as either sternal or lateral recumbency with ≥ 75% of the piglet’s body touching the heat mat or inside the lamp heat circle. Production records were used to verify farrowing date, total number of piglets born, and born alive. No cross fostering occurred during this study. All data will be presented descriptively. On average, sows assigned to the LAMP treatment took ~2 ½-h to farrow, and for sows assigned to the MAT ~3 ½-h, respectively. Piglets took between ~5-h (LAMP) and ~9-h (MAT) for ≥ 50% of piglets to locate and lie on- or under the supplemental heat source for ≥ 5 min after the birth of the last piglet. Cohort of piglets laid on- or under the heat source as follows, LAMP piglets spent ~29 mins lying and for MAT piglets ~42 mins, respectively. Average pre-weaning mortality was 11% (LAMP) and 18% (MAT). The MAT heat source used less energy than the LAMP (16 vs. 63 kWh) over the study duration. To the authors’ knowledge, this is the first published study using a continuous sampling method to precisely examine a new measure (time needed for ≥ 50% of piglets to locate and lie on- or under the supplemental heat source for ≥ 5 min after the birth of the last-born piglet) and to determine how long this cohort of piglets laid on- or under the heat source. Our findings show an immense range in locating and lying under- or on the heat source. Therefore, we suggest that caretakers should assist all piglets to locate the heat source after farrowing is complete to improve piglet livability.
Darkness promotes dishonesty in a coin toss task: A pre-registered conceptual replication of Experiment 1 of Zhong, Bohns, and Gino (2010)
Authors: Liu Huanxu , Zhou Yuning , Yamada Yuki
Published: December 2023

An earlier study suggested that individuals tend to be more dishonest in darker environments, this phenomenon was attributed to an increase in participants’ perceived anonymity. However, instead of using quantifiable measurements, the original experiment depended on the experimenter’s subjective observation to construct different brightness conditions, which led to a less precise understanding of the phenomenon. Additionally, the task used in the original experiment has recently been criticized as being unsuitable for dishonesty-detection studies. This study addressed these concerns to retest the effect of brightness on dishonest behavior. This study employed lux as a unit to accurately control the brightness within the experiment room. Moreover, the coin toss task which is frequently employed in dishonesty-detection experiments, was utilized instead of the task in the original experiment. The findings revealed that despite altering the content of the task, dishonesty increased in the dark condition. Contrary to the findings in the original experiment, however, the results did not substantiate that perceived anonymity was the driving factor of the effect of brightness on dishonesty. This discrepancy suggests that further empirical considerations are warranted to unravel the underlying mechanisms.
RiNeo MR: A mixed reality simulator for newborn life support training
Authors: Coduri Mara , Calandrino Andrea , Addiego Mobilio Giulia , Casadio Maura , Ricci Serena
Published: December 2023

Neonatal resuscitation is an uncommon, albeit critical task that is more likely to succeed if performed properly and promptly. In this context, simulation is an appropriate way for training and assessing the abilities of all medical staff involved in delivery room care. Recent studies have shown that learning is enhanced if the simulation experience is realistic and engaging. Hence, Virtual Reality can be beneficial for newborn resuscitation training. However, the difficulty of providing realistic haptic interaction limits its use. To overcome this constraint, we have designed RiNeo MR, a simulator for newborn life support training, combining a sensorized manikin to monitor in real time resuscitation skills, with a Virtual Reality application. The system includes a Virtual Reality headset, Leap Motion to track the user’s hands, sensorized bag valve mask, and manikin to monitor head and mask positioning, ventilation, and chest compression. RiNeo MR can be used in two modalities: 2D to let the trainee practice resuscitation manoeuvres on the physical manikin, while receiving real time feedback; 3D that allows the user to be immersed in a virtual environment and practice in an hospital-like setting. In the 3D mode, virtual and real manikins are overlapped and communicate in real time. Tests on 16 subjects (11 controls without medical expertise and 5 paediatric residents) demonstrated that the simulator is well tolerated in terms of discomfort. Moreover, the simulator is high rated for user experience and system usability, suggesting that RiNeo MR can be a promising tool to improve newborn life support training. RiNeo MR is a proof of concept of a mixed-reality newborn life support simulator that can be a promising tool to spread newborn resuscitation high-quality training among healthcare providers involved in perinatal medicine.
Fast cycling culture of the annelid model Platynereis dumerilii
Authors: Legras Mathieu , Ghisleni Giulia , Regnard Léna , Dias Manon , Soilihi Rabouant , Celmar Enzo , Balavoine Guillaume
Published: December 2023

Platynereis dumerilii, a marine annelid, is a model animal that has gained popularity in various fields such as developmental biology, biological rhythms, nervous system organization and physiology, behaviour, reproductive biology, and epigenetic regulation. The transparency of P. dumerilii tissues at all developmental stages makes it easy to perform live microscopic imaging of all cell types. In addition, the slow-evolving genome of P. dumerilii and its phylogenetic position as a representative of the vast branch of Lophotrochozoans add to its evolutionary significance. Although P. dumerilii is amenable to transgenesis and CRISPR-Cas9 knockouts, its relatively long and indefinite life cycle, as well as its semelparous reproduction have been hindrances to its adoption as a reverse genetics model. To overcome this limitation, an adapted culturing method has been developed allowing much faster life cycling, with median reproductive age at 13–14 weeks instead of 25–35 weeks using the traditional protocol. A low worm density in boxes and a strictly controlled feeding regime are important factors for the rapid growth and health of the worms. This culture method has several advantages, such as being much more compact, not requiring air bubbling or an artificial moonlight regime for synchronized sexual maturation and necessitating only limited water change. A full protocol for worm care and handling is provided.
Thiophene derivative inflicts cytotoxicity via an intrinsic apoptotic pathway on human acute lymphoblastic leukemia cells
Authors: Swain Risa Mia , Sanchez Anahi , Gutierrez Denisse A. , Varela-Ramirez Armando , Aguilera Renato J.
Published: December 2023

In an effort to identify novel anti-cancer agents, we employed a well-established High Throughput Screening (HTS) assay to assess the cytotoxic effect of compounds within the ChemBridge DIVERSet Library on a lymphoma cell line. This screen revealed a novel thiophene, F8 (methyl 5-[(dimethylamino)carbonyl]-4-methyl-2-[(3-phenyl-2-propynoyl) amino]-3-thiophenecarboxylate), that displays anti-cancer activity on lymphoma, leukemia, and other cancer cell lines. Thiophenes and thiophene derivatives have emerged as an important class of heterocyclic compounds that have displayed favorable drug characteristics. They have been previously reported to exhibit a broad spectrum of properties and varied uses in the field of medicine. In addition, they have proven to be effective drugs in various disease scenarios. They contain anti-inflammatory, anti-anxiety, anti-psychotic, anti-microbial, anti-fungal, estrogen receptor modulating, anti-mitotic, kinase inhibiting and anti-cancer activities, rendering compounds with a thiophene a subject of significant interest in the scientific community. Compound F8 consistently induced cell death at a low micromolar range on a small panel of cancer cell lines after a 48 h period. Further investigation revealed that F8 induced phosphatidylserine externalization, reactive oxygen species generation, mitochondrial depolarization, kinase inhibition, and induces apoptosis. These findings demonstrate that F8 has promising anti-cancer activity.
Living normally without being oneself: A qualitative study on the experience of living with advanced chronic kidney disease
Authors: de la Cuesta-Benjumea Carmen , Hernádez-Ibarra Luis Eduardo , Arredondo-González Claudia P.
Published: December 2023

The aim of the study was to describe and analyze the experience of people with advanced chronic kidney disease. Chronic kidney disease is a growing public health problem that is on the increase worldwideThe experience of living with this illness is paradoxical, as it can include feelings of dependent autonomy, distant connection, abnormal normalcy, and uncertain hope. Every chronic disease involves a biographical alteration from the onset. For those who suffer it, it implies a breaking down and reconstruction of their everyday life. Despite the prevalence of the disease and the increase in the number of qualitative research studies in recent decades, there has not been much research on the experience of people with Chronic kidney disease. This is a qualitative study that began in 2018 and concluded in 2021.Twenty-one people with advanced chronic kidney disease were interviewed. They participated voluntarily with informed consent Participants were selected by purposive sampling. Data analysis was guided by grounded theory procedures using the Nvivo 12 software. This study reveals that people with advanced chronic kidney disease do not feel the same as they used to because their control over their lives is limited; because they feel their health is in a continuous state of deterioration; and because of the changes in themselves and in their relationships with others. With chronic kidney disease, their identity is continually called into question. The normal lives of these people–their biographical constructions–are precarious and are continuously being remodelled by the effects of treatment and the inexorable course of this disease. This study contributes to an understanding of the experience of people with advanced chronic kidney disease. It can contribute to helping health care professionals effectively support these patients in their efforts to lead a normal life and in making decisions about their treatment.
The effects of zinc sulfate on mycelial enzyme activity and metabolites of Pholiota adiposa
Authors: Ma Xiao-ying , Yang Tao , Xiao Jun , Zhang Peng
Published: December 2023

The aim of this study was to investigate the effect of zinc sulphate on the activities of different enzymes and metabolites of Pholiota adiposa. In the experiment, we used the conventional enzyme activity assay to determine the changes of six indicators, including protein content, laccase activity, cellulase activity, amylase activity and polyphenol oxidase activity, under different concentrations of zinc sulphate treatment. The results showed that the activities of amylase, laccase, cellulase and peroxidase were Zn2+(200)>Zn2+(0)>Zn2+(400)>Zn2+(800).The activities of catalase and superoxide dismutase were Zn2+(200)>Zn2+(400)>Zn2+(800), and zinc sulfate could significantly affect the activity of polylipic squamase in a dose-dependent manner. Further correlation analysis showed that all six enzyme activities were significantly correlated with each other (P<001); the results of the statistical model test showed that the regression model constructed was statistically significant; overall the residuals met the conditions of normal distribution, and the corresponding points of different enzyme activities Q—Q’ were more evenly distributed around y = x, and all fell in the 90% acceptance interval, thus the series was considered to obey normal distribution; the results of the principal The results of the principal component analysis showed that principal component 1 was positively correlated with amylase, laccase and cellulase. Principal component 2 was positively correlated with superoxide dismutase and catalase, and negatively correlated with peroxidase. The analysis of Metabonomic data revealed that zinc sulfate had a significant impact on the expression of metabolites in the mycelium. Moreover, varying concentrations of zinc sulfate exerted significant effects on the levels of amino acids, organic acids, and gluconic acid. This conclusion was confirmed by other experimental data. The results of the study provide a scientific reference for better research, development and utilization of Pholiota adiposa.
COVID-19 prediction using Caviar Squirrel Jellyfish Search Optimization technique in fog-cloud based architecture
Authors: Amgothu Shanthi , Koppu Srinivas
Published: December 2023

In the pandemic of COVID-19 patients approach to the hospital for prescription, yet due to extreme line up the patient gets treatment after waiting for more than one hour. Generally, wearable devices directly measure the preliminary data of the patient stored in capturing mode. In order to store the data, the hospitals require large storage devices that make the progression of data more complex. To bridge this gap, a potent scheme is established for COVID-19 prediction based fog-cloud named Caviar Squirrel Jellyfish Search Optimization (CSJSO). Here, CSJSO is the amalgamation of CAViar Squirrel Search Algorithm (CSSA) and Jellyfish Search Optimization (JSO), where CSSA is blended by the Conditional Autoregressive Value-at-Risk (CAViar) and Squirrel Search Algorithm (SSA). This architecture comprises the healthcare IoT sensor layer, fog layer and cloud layer. In the healthcare IoT sensor layer, the routing process with the collection of patient health condition data is carried out. On the other hand, in the fog layer COVID-19 detection is performed by employing a Deep Neuro Fuzzy Network (DNFN) trained by the proposed Remora Namib Beetle JSO (RNBJSO). Here, RNBJSO is the combination of Namib Beetle Optimization (NBO), Remora Optimization Algorithm (ROA) and Jellyfish Search optimization (JSO). Finally, in the cloud layer, the detection of COVID-19 employing Deep Long Short Term Memory (Deep LSTM) trained utilizing proposed CSJSO is performed. The evaluation measures utilized for CSJSO_Deep LSTM in database-1, such as Mean Squared Error (MSE) and Root Mean Squared Error (RMSE) observed 0.062 and 0.252 in confirmed cases. The measures employed in database-2 are accuracy, sensitivity and specificity achieved 0.925, 0.928 and 0.925 in K-set.
Impact of school SES on literacy development
Authors: Salas Naymé , Pascual Mariona
Published: December 2023

Effective literacy skills are essential to actively participate in today’s society. However, little research has been conducted that examined the impact of contextual variables on literacy development. The present paper addressed whether and how the socioeconomic status of the school (S-SES) children attend affects their literacy achievements. Eight-hundred and seventy-eight 2nd and 4th grade children participated in the study. Data were collected in low-SES (vulnerable) and in mid-high-SES (non-vulnerable) schools. Children completed a large battery of language, cognitive, and literacy tasks in Catalan, a language spoken in a region in Spain where virtually all children are at least bilingual (they also speak Spanish) and it is the main language of instruction. Results showed that children in vulnerable schools were outperformed by children in non-vulnerable schools across all literacy competencies, but particularly affected higher order skills; that is, text quality and reading comprehension. Differences with their non-vulnerable peers remained, even after controlling for context-level covariates, including familial SES. However, S-SES ceased to exert significant influence once children’s cognitive and, especially, linguistic skills were considered. The study adds to previous research claiming that school SES has an effect on students’ literacy skills, above and beyond children’s home SES. However, our findings also suggest that literacy performance is ultimately mostly dependent on educationally actionable, subject-level skills. Educational implications are discussed.
Controlling the counterintuitive optical repulsive thrust of nano dimers with counter propagating type waves and background medium
Authors: Biswas Sudipta , Mahdy M. R. C. , Das Saikat Chandra , Bhuiyan Md. Ariful Islam , Talukder Mohammad Abir
Published: December 2023

This work focuses on the utilization of counter-propagating plane waves for optical manipulation, which provides a unique approach to control the behavior of Rayleigh and Dipolar nanoparticles immersed in a homogeneous or heterogeneous medium. Our study presents an interesting finding of a repulsive force between plasmonic-chiral heterodimers where the particles move away from each other in both near and far field regions. Interestingly, this repulsive thrust supports the wave like nature of light for the case of homogeneous background but particle type nature of light for heterogenous background. At first, we have investigated the theory underlying the optical trapping of the chiral particle and the impact of this phenomenon on the overall repulsive behavior of the heterodimers placed in air (homogeneous) background. After that, our proposed set-up has further been investigated putting in air-water interface (heterogenous background) and by varying light angle only a little bit. Our observation for this interface case is suggesting the transfer of Minkowski momentum of photon to each optically pulled Rayleigh or dipolar particle of the dimer set, which ultimately causes a broad-band giant repulsive thrust of the dimers. However, in absence of the other particle in the cluster, a single half-immersed particle does not experience the pulling force for the broad-band spectrum. The ‘common’ reason of the observed repulsive thrust of the dimers for both the aforementioned cases has been attributed to "modified" longitudinal Optical Binding Force (OBF). Technically, this work may open a new way to control the repulsion and attraction between the nanoparticles both in near and far field regions by utilizing the background and the counter-propagating waves. We also believe that this work manifests a possible simple set-up, which will support to observe a background dependent wave ‘or’ particle nature of light experimentally.
Impact of the digital economy on urban green growth: Empirical evidence from China
Authors: Ren Yangjun , Tian Ze , Liu Chao
Published: December 2023

Using the digital economy to empower urban economic green growth provides essential opportunities for China to achieve high-quality growth. This paper assesses the level of digital economy and green growth in Chinese cities, seeking to explore the mechanisms and effects of the digital economy on urban green growth in a unified framework. The results indicate that the digital economy can drive cities’ green growth. This conclusion still holds after a set of robustness tests. Meanwhile, the green value of the digital economy is fully released among the eastern cities, major urban agglomerations, and high-level cities. Further research shows that the digital economy can indirectly enhance urban green growth in the neighboring regions through spatial spillover effects. Moreover, labor resource mismatch, capital resource mismatch and green technology innovation are significant mediating mechanisms. The findings could guide policymakers on green growth in emerging economies from a digital economy perspective.
Stillbirth incidence and determinants in a tertiary health facility in the Volta Region of Ghana
Authors: Dah Anthony Kwame , Osarfo Joseph , Ampofo Gifty Dufie , Appiah-Kubi Adu , Mbroh Hintermann , Azanu Wisdom Klutse , Sakyi Afia Tabuaa , Abradu Lydia , Morhe Emmanuel Senanu Komla
Published: December 2023

Background

Stillbirths are indicators of the quality of obstetrics care in health systems. Stillbirth rates and their associating factors vary by socio-economic and geographical settings. Published data on stillbirths and their associating factors in the Volta Region of Ghana are limited. This limits understanding of local factors that must be considered in designing appropriate interventions to mitigate the occurrence of stillbirths. This study determined the incidence of stillbirths and associated factors among deliveries at Ho Teaching Hospital (HTH) and contributes to understanding the consistent high stillbirths in the country and potentially in other low-resourced settings in sub-Saharan Africa.

Method

This was a prospective cohort study involving pregnant women admitted for delivery at HTH between October 2019 and March 2020. Data on socio-demographic characteristics such as age and employment, obstetric factors including gestational age at delivery and delivery outcomes like birthweight were collected using a pretested structured questionnaire. The primary outcome was the incidence of stillbirths at the facility. Summary statistics were reported as frequencies, percentages and means. Logistic regression methods were used to assess for association between stillbirths and independent variables including age and birthweight. Odds ratios were reported with 95% confidence intervals and associations with p-values < 0.05 were considered statistically significant.

Results

A total of 687 women and their 702 newborns contributed data for analysis. The mean age (SD) was 29.3 (6.3) years and close to two-thirds had had at least one delivery previously. Overall stillbirth incidence was 31.3 per 1000 births. Of the 22 stillbirths, 17 were antepartum. Pre-eclampsia was the most common hypertensive disorder of pregnancy observed (49.3%, 33/67). Among others, less than 3 antenatal visits and low birthweight increased the odds of stillbirths in the bivariate analysis. In the final multivariate model, pregnancy and delivery at 28–34 weeks gestation [AOR 9.37(95% CI 1.18–74.53); p = 0.034] and induction of labour [AOR 11.06 (95% CI 3.10–39.42); p < 0.001] remained significantly associated with stillbirths.

Conclusion

Stillbirth incidence was 31.3 per 1000 births with more than half being antepartum stillbirths. Pregnancy/delivery at 28–34 weeks’ gestation increased the odds of a stillbirth. Improving the quality of antenatal services, ensuring adherence to evidence-based protocols, accurate and prompt diagnosis and timely interventions of medical conditions in pregnancy particularly at 28–34 weeks’ gestation could reduce incidence of stillbirths.
ATXN2 is a target of N-terminal proteolysis
Authors: Chitre Monika , Emery Patrick
Published: December 2023

Spinocerebellar ataxia 2 (SCA2) is a neurodegenerative disorder caused by the expansion of the poly-glutamine (polyQ) tract of Ataxin-2 (ATXN2). Other polyQ-containing proteins such as ATXN7 and huntingtin are associated with the development of neurodegenerative diseases when their N-terminal polyQ domains are expanded. Furthermore, they undergo proteolytic processing events that produce N-terminal fragments that include the polyQ stretch, which are implicated in pathogenesis. Interestingly, N-terminal ATXN2 fragments were reported in a brain extract from a SCA2 patient, but it is currently unknown whether an expanded polyQ domain contributes to ATXN2 proteolytic susceptibility. Here, we used transient expression in HEK293 cells to determine whether ATXN2 is a target for specific N-terminal proteolysis. We found that ATXN2 proteins with either normal or expanded polyQ stretches undergo proteolytic cleavage releasing an N-terminal polyQ-containing fragment. We identified a short amino acid sequence downstream of the polyQ domain that is necessary for N-terminal cleavage of full-length ATXN2 and sufficient to induce proteolysis of a heterologous protein. However, this sequence is not required for cleavage of a short ATXN2 isoform produced from an alternative start codon located just upstream of the CAG repeats encoding the polyQ domain. Our study extends our understanding of ATXN2 posttranslational regulation by revealing that this protein can be the target of specific proteolytic cleavage events releasing polyQ-containing products that are modulated by the N-terminal domain of ATXN2. N-terminal ATXN2 proteolysis of expanded polyQ domains might contribute to SCA2 pathology, as observed in other neurodegenerative disorders caused by polyQ domain expansion.
Random forest classification as a tool in epidemiological modelling: Identification of farm-specific characteristics relevant for the occurrence of Fasciola hepatica on German dairy farms
Authors: Oehm Andreas W. , Zablotski Yury , Campe Amely , Hoedemaker Martina , Strube Christina , Springer Andrea , Jordan Daniela , Knubben-Schweizer Gabriela
Published: December 2023

Fasciola hepatica is an internal parasite of both human and veterinary relevance. In order to control fasciolosis, a multitude of attempts to predict the risk of infection such as risk maps or forecasting models have been developed. These attempts mainly focused on the influence of geo-climatic and meteorological features. Predicting bovine fasciolosis on farm level taking into account farm-specific settings yet remains challenging. In the present study, a new methodology for this purpose, a data-driven machine learning approach using a random forest classification algorithm was applied to a cross-sectional data set of farm characteristics, management regimes, and farmer aspects within two structurally different dairying regions in Germany in order to identify factors relevant for the occurrence of F. hepatica that could predict farm-level bulk tank milk positivity. The resulting models identified farm-specific key aspects in regard to the presence of F. hepatica. In study region North, farm-level production parameters (farm-level milk yield, farm-level milk fat, farm-level milk protein), leg hygiene, body condition (prevalence of overconditioned and underconditioned cows, respectively) and pasture access were identified as features relevant in regard to farm-level F. hepatica positivity. In study region South, pasture access together with farm-level lameness prevalence, farm-level prevalence of hock lesions, herd size, parity, and farm-level milk fat appeared to be important covariates. The stratification of the analysis by study region allows for the extrapolation of the results to similar settings of dairy husbandry. The local, region-specific modelling of F. hepatica presence in this work contributes to the understanding of on-farm aspects of F. hepatica appearance. The applied technique represents a novel approach in this context to model epidemiological data on fasciolosis which allows for the identification of farms at risk and together with additional findings in regard to the epidemiology of fasciolosis, can facilitate risk assessment and deepen our understanding of on-farm drivers of the occurrence of F. hepatica.
Barriers and enablers to and strategies for promoting domestic plasma donation throughout the world: Overarching protocol for three systematic reviews
Authors: Etherington Cole , Palumbo Amelia , Holloway Kelly , Meyer Samantha , Labrecque Maximillian , Rubini Kyle , Shorr Risa , Welch Vivian , Gibson Emily , Foster Terrie , Haw Jennie , Vesnaver Elisabeth , Maharshi Manavi T. , O’Brien Sheila F. , MacPherson Paul , Dogba Joyce , Steed Tony , Goldman Mindy , Presseau Justin
Published: December 2023

Introduction

The growing demand for plasma protein products has caused concern in many countries who largely rely on importing plasma products produced from plasma collected in the United States and Europe. Optimizing recruitment and retention of a diversity of plasma donors is therefore important for supporting national donation systems that can reliably meet the most critical needs of health services. This series of three systematic reviews aims to synthesize the known barriers and enablers to source plasma donation from the qualitative and survey-based literature and identify which strategies that have shown to be effective in promoting increased intention to, and actual donation of, source plasma.

Methods and analysis

Primary studies involving source or convalescent plasma donation via plasmapheresis will be included. The search strategy will capture all potentially relevant studies to each of the three reviews, creating a database of plasma donation literature. Study designs will be subsequently identified in the screening process to facilitate analysis according to the unique inclusion criteria of each review (i.e., qualitative, survey, and experimental designs). The search will be conducted in the electronic databases SCOPUS, MEDLINE, EMBASE, PsycINFO, and CINAHL without date or language restrictions. Studies will be screened, and data will be extracted, in duplicate by two independent reviewers with disagreements resolved through consensus. Reviews 1 and 2 will draw on the Theoretical Domains Framework and Intersectionality, while Review 3 will be informed by Behaviour Change Intervention Ontologies. Directed content analysis and framework analysis (Review 1), and descriptive and inferential syntheses (Reviews 2 and 3), will be used, including meta-analyses if appropriate.

Discussion

This series of related reviews will serve to provide a foundation of what is known from the published literature about barriers and enablers to, and strategies for promoting, plasma donation worldwide.
Spatial distribution and circadian locomotor activity of invasive armored catfish (Loricariidae) in the freshwater and brackish water
Authors: Pavlov Efim D. , Dien Tran Duc , Ganzha Ekaterina V.
Published: December 2023

Invasive suckermouth armored catfish Pterygoplichthys spp. successfully spread during one decade into many river systems of Vietnam. Wide and rapid invasion of armored catfish might be associated with using brackish water in estuaries to spread from one river system to another. The first goal of our study was to assess the horizontal and vertical distribution of invasive fish in freshwater (Da Rang River) and in brackish water (Da Rang River estuary) associated with circadian rhythm. In the both sampling locations, fish were mainly caught at nighttime at the bottom and near the surface using the net traps and vertical nets. In estuary, fish were caught in the net traps with distance 2.0–7.5 m from the right or left banks where water was predominantly fresh. In freshwater of the Da Rang River, fish were often caught near the left bank with gravel and stone substrate. The second goal of our study was to experimentally evaluate the circadian (12 hours of the night and 12 hours of the day) rhythm of locomotor activity (LA) of fish. Fish from different freshwater locations (Am Chua canal and Da Rang River) had a similar diurnal dynamic of LA with mostly movements (77–83% of total diurnal LA) at nighttime (18:00–6:00, GMT+7) at the end of the wet season. Armored catfish from the brackish water location (Da Rang River estuary) also were mostly active (76% of total diurnal LA) overnight. However, fish from freshwater and brackish water had difference in the timing of behavioral activity. Fish LA from estuary was significantly lower than LA of fish from freshwater locations during 18:00 to 21:00 before low tide. The results of our field and experimental studies established that armored catfish in estuary moved in horizontal and vertical planes predominantly at nighttime. Tide level regulates locomotor activity of invasive fish and could influence on the possibility of their spreading through the estuary.
How predictive of SARS-CoV-2 infection are clinical characteristics at presentation among individuals with COVID-like symptoms treated at the Mexican Institute of Social Security
Authors: Gutierrez Juan Pablo , Olaiz Gustavo , Juárez-Flores Arturo , Borja-Aburto Víctor H. , Ascencio-Montiel Iván J. , Bertozzi Stefano M.
Published: December 2023

Background

The COVID-19 pandemic has progressed rapidly, with the emergence of new virus variants that pose challenges in treating infected individuals. In Mexico, four epidemic waves have been recorded with varying disease severity. To understand the heterogeneity in clinical presentation over time and the sensitivity and specificity of signs and symptoms in identifying COVID-19 cases, an analysis of the changes in the clinical presentation of the disease was conducted.

Aim

To analyze the changes in the clinical presentation of COVID-19 among 3.38 million individuals tested for SARS-CoV-2 at the Mexican Social Security Institute (IMSS) from March 2020 to October 2021 and evaluate the predictivity of signs and symptoms in identifying COVID-19 cases.

Methods

A retrospective analysis of clinical presentation patterns of COVID-19 among individuals treated at IMSS was performed, contrasting the signs and symptoms among SARS-CoV-2-positive individuals with those who tested negative for the virus but had respiratory infection symptoms. The sensitivity and specificity of each sign and symptom in identifying SARS-CoV-2 infection were estimated.

Results

The set of signs and symptoms reported for COVID-19-suspected patients treated at IMSS were not highly specific for SARS-CoV-2 positivity. The signs and symptoms exhibited variability based on age and epidemic wave. The area under the receiver operating characteristic (ROC) curve was 0.62 when grouping the five main symptoms (headache, dyspnea, fever, arthralgia, and cough). Most of the individual symptoms had ROC values close to 0.5 (16 out of 22 between 0.48 and 0.52), indicating non-specificity.

Conclusions

The results highlight the difficulty in making a clinical diagnosis of COVID-19 due to the lack of specificity of signs and symptoms. The variability of clinical presentation over time and among age groups highlights the need for further research to differentiate whether the changes are due to changes in the virus, who is becoming infected, or the population, particularly with respect to prior infection and vaccination status.
FLAgellum Member 8 modulates extravascular distribution of African trypanosomes
Authors: Calvo-Alvarez Estefanía , Ngoune Jean Marc Tsagmo , Sharma Parul , Cooper Anneli , Camara Aïssata , Travaillé Christelle , Crouzols Aline , MacLeod Annette , Rotureau Brice
Published: December 2023

In the mammalian host, the biology of tissue-dwellingTrypanosoma bruceiparasites is not completely understood, especially the mechanisms involved in their extravascular colonization. The trypanosome flagellum is an essential organelle in multiple aspects of the parasites’ development. The flagellar protein termed FLAgellar Member 8 (FLAM8) acts as a docking platform for a pool of cyclic AMP response protein 3 (CARP3) that is involved in signaling. FLAM8 exhibits a stage-specific distribution suggesting specific functions in the mammalian and vector stages of the parasite. Analyses of knockdown and knockout trypanosomes in their mammalian forms demonstrated that FLAM8 is not essentialin vitrofor survival, growth, motility and stumpy differentiation. Functional investigations in experimental infections showed thatFLAM8-deprived trypanosomes can establish and maintain an infection in the blood circulation and differentiate into insect transmissible forms. However, quantitative bioluminescence imaging and gene expression analysis revealed thatFLAM8-null parasites exhibit a significantly impaired dissemination in the extravascular compartment, that is restored by the addition of a single rescue copy ofFLAM8.In vitrotrans-endothelial migration assays revealed significant defects in trypanosomes lackingFLAM8. FLAM8 is the first flagellar component shown to modulateT.bruceidistribution in the host tissues, possibly through sensing functions, contributing to the maintenance of extravascular parasite populations in mammalian anatomical niches, especially in the skin.
A sticky situation: When trypanosomatids attach to insect tissues
Authors: Povelones Megan L. , Holmes Nikki A. , Povelones Michael
Published: December 2023

Transmission of trypanosomatids to their mammalian hosts requires a complex series of developmental transitions in their insect vectors, including stable attachment to an insect tissue. While there are many ultrastructural descriptions of attached cells, we know little about the signaling events and molecular mechanisms involved in this process. Each trypanosomatid species attaches to a specific tissue in the insect at a particular stage of its life cycle. Attachment is mediated by the flagellum, which is modified to accommodate a filament-rich plaque within an expanded region of the flagellar membrane. Attachment immediately precedes differentiation to the mammal-infectious stage and in some cases a direct mechanistic link has been demonstrated. In this review, we summarize the current state of knowledge of trypanosomatid attachment in insects, including structure, function, signaling, candidate molecules, and changes in gene expression. We also highlight remaining questions about this process and how the field is poised to address them through modern approaches.