bluuebunny/crossref_metadata_2025 huggingface dataset:
Season Affects Yield and Metabolic Profiles of Rice (Oryza sativa) under High Night Temperature Stress in the Field
Authors: Schaarschmidt Stephanie , Lawas Lovely Mae F. , Glaubitz Ulrike , Li Xia , Erban Alexander , Kopka Joachim , Jagadish S. V. Krishna , Hincha Dirk K. , Zuther Ellen
Published: May 2020

Rice (Oryza sativa) is the main food source for more than 3.5 billion people in the world. Global climate change is having a strong negative effect on rice production. One of the climatic factors impacting rice yield is asymmetric warming, i.e., the stronger increase in nighttime as compared to daytime temperatures. Little is known of the metabolic responses of rice to high night temperature (HNT) in the field. Eight rice cultivars with contrasting HNT sensitivity were grown in the field during the wet (WS) and dry season (DS) in the Philippines. Plant height, 1000-grain weight and harvest index were influenced by HNT in both seasons, while total grain yield was only consistently reduced in the WS. Metabolite composition was analysed by gas chromatography-mass spectrometry (GC-MS). HNT effects were more pronounced in panicles than in flag leaves. A decreased abundance of sugar phosphates and sucrose, and a higher abundance of monosaccharides in panicles indicated impaired glycolysis and higher respiration-driven carbon losses in response to HNT in the WS. Higher amounts of alanine and cyano-alanine in panicles grown in the DS compared to in those grown in the WS point to an improved N-assimilation and more effective detoxification of cyanide, contributing to the smaller impact of HNT on grain yield in the DS.
Tubulin Folding Cofactor TBCB is a Target of the Salmonella Effector Protein SseK1
Authors: Araujo-Garrido Juan Luis , Baisón-Olmo Fernando , Bernal-Bayard Joaquín , Romero Francisco , Ramos-Morales Francisco
Published: May 2020

Salmonella enterica serovar Typhimurium is a human and animal pathogen that uses type III secretion system effectors to manipulate the host cell and fulfill infection. SseK1 is a Salmonella effector with glycosyltransferase activity. We carried out a yeast two-hybrid screen and have identified tubulin-binding cofactor B (TBCB) as a new binding partner for this effector. SseK1 catalyzed the addition of N-acetylglucosamine to arginine on TBCB, and its expression promoted the stabilization of the microtubule cytoskeleton of HEK293T cells. The conserved Asp-x-Asp (DxD) motif that is essential for the activity of SseK1 was required for the binding and modification of TBCB and for the effect on the cytoskeleton. Our study has identified a novel target for SseK1 and suggests that this effector may have a role in the manipulation of the host cell microtubule network to provide a safe niche for this pathogen.
Limited Proteolysis of Cyclooxygenase-2 Enhances Cell Proliferation
Authors: Saadi Esraa , Sood Rapita , Dromi Ido , Srouji Ranin , Abu Hatoum Ossama , Tal Sharon , Barki-Harrington Liza
Published: May 2020

Accumulating evidence suggests that the cyclooxygenase-2 (COX-2) enzyme has additional catalytic-independent functions. Here we show that COX-2 appears to be cleaved in mouse and human tumors, which led us to hypothesize that COX-2 proteolysis may play a role in cell proliferation. The data presented herein show that a K598R point mutation at the carboxyl-terminus of COX-2 causes the appearance of several COX-2 immunoreactive fragments in nuclear compartments, and significantly enhances cell proliferation. In contrast, insertion of additional mutations at the border of the membrane-binding and catalytic domains of K598R COX-2 blocks fragment formation and prevents the increase in proliferation. Transcriptomic analyses show that K598R COX-2 significantly affects the expression of genes involved in RNA metabolism, and subsequent proteomics suggest that it is associated with proteins that regulate mRNA processing. We observe a similar increase in proliferation by expressing just that catalytic domain of COX-2 (ΔNT- COX-2), which is completely devoid of catalytic activity in the absence of its other domains. Moreover, we show that the ΔNT- COX-2 protein also interacts in the nucleus with β-catenin, a central regulator of gene transcription. Together these data suggest that the cleavage products of COX-2 can affect cell proliferation by mechanisms that are independent of prostaglandin synthesis.
Short Exposure to Ethanol Diminishes Caspase-1 and ASC Activation in Human HepG2 Cells In Vitro
Authors: Hörauf Jason-Alexander , Kany Shinwan , Janicova Andrea , Xu Baolin , Vrdoljak Teodora , Sturm Ramona , Dunay Ildiko Rita , Martin Lukas , Relja Borna
Published: May 2020

This paper discusses how the assembly of pro-caspase-1 and apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC) in macromolecular protein complexes, inflammasomes, activates caspase-1. The present study investigates the molecular mechanisms of inflammasome activation in HepG2 cells and examines how short exposures to ethanol (EtOH) affect inflammasome activation. HepG2 cells were treated with lipopolysaccharide (LPS), ATP or nigericin (NIG) in a two-step model. After LPS priming, ATP or NIG were added. As inhibitors, sodium orthovanadate (general inhibitor of tyrosine phosphatases), AC-YVAD-CMK (caspase-1 inhibitor) or AZ10606120 (purinergic receptor P2X7R inhibitor) were applied after LPS priming. To monitor the inflammasome activation, the caspase-1 activity, ASC speck formation, reactive oxygen species (ROS) production and cell death were analyzed. To elucidate the mechanistical approach of EtOH to the inflammasome assembly, the cells were treated with EtOH either under simultaneous LPS administration or concurrently with ATP or NIG application. The co-stimulation with LPS and ATP induced a significant ASC speck formation, caspase-1 activation, cell death and ROS generation. The inhibition of the ATP-dependent purinoreceptor P2X7 decreased the caspase-1 activation, whereas sodium orthovanadate significantly induced caspase-1. Additional treatment with EtOH reversed the LPS and ATP-induced caspase-1 activation, ASC speck formation and ROS production. The ASC speck formation and caspase-1 induction require a two-step signaling with LPS and ATP in HepG2 cells. Inflammasome activation may depend on P2X7. The molecular pathway of an acute effect of EtOH on inflammasomes may involve a reduction in ROS generation, which in turn may increase the activity of tyrosine phosphatases.
The Novel ncRNA OsiR Positively Regulates Expression of katE2 and is Required for Oxidative Stress Tolerance in Deinococcus radiodurans
Authors: Gao Lihua , Chen Xiaonan , Tian Ye , Yan Yongliang , Zhan Yuhua , Zhou Zhengfu , Zhang Wei , Lin Min , Chen Ming
Published: May 2020

Deinococcus radiodurans is a polyextremophilic bacterium well known for its extreme resistance to irradiation, oxidative stress, and other damaging conditions. Many small noncoding RNAs (ncRNAs) in D. radiodurans have been identified by deep sequencing analysis and computational predictions. However, the precise roles of ncRNAs and their target genes in the oxidative stress response have not been investigated. Here, we report the identification and characterization of a novel ncRNA named OsiR (for oxidative stress-induced ncRNA). Oxidative stress tolerance analysis showed that deleting osiR significantly decreased viability, total antioxidant capacity, and catalase activity in D. radiodurans under oxidative stress conditions. Comparative phenotypic and qRT-PCR analyses of an osiR mutant identify a role of OsiR in regulating the expression of the catalase gene katE2. Microscale thermophoresis and genetic complementation showed that a 21-nt sequence in the stem–loop structure of OsiR (204–244 nt) directly base pairs with its counterpart in the coding region of katE2 mRNA (843–866 nt) via a 19 nt region. In addition, deletion of katE2 caused a significant reduction of catalase activity and oxidative stress tolerance similar to that observed in an osiR mutant. Our results show that OsiR positively regulates oxidative stress tolerance in D. radiodurans by increasing the mRNA stability and translation efficiency of katE2. This work provides a new regulatory pathway mediated by ncRNA for the oxidative stress response that most likely contributes to the extreme tolerances of D. radiodurans.
High Expression of microRNA-143 is Associated with Favorable Tumor Immune Microenvironment and Better Survival in Estrogen Receptor Positive Breast Cancer
Authors: Tokumaru Yoshihisa , Asaoka Mariko , Oshi Masanori , Katsuta Eriko , Yan Li , Narayanan Sumana , Sugito Nobuhiko , Matsuhashi Nobuhisa , Futamura Manabu , Akao Yukihiro , Yoshida Kazuhiro , Takabe Kazuaki
Published: May 2020

microRNA-143 (miR-143) is a well-known tumor suppressive microRNA that exhibits anti-tumoral function by targeting KRAS signaling pathways in various malignancies. We hypothesized that miR-143 suppresses breast cancer progression by targeting KRAS and its effector molecules. We further hypothesized that high expression of miR-143 is associated with a favorable tumor immune microenvironment of estrogen receptor (ER)-positive breast cancer patients which result in improved survival. Two major publicly available breast cancer cohorts; The Cancer Genome Atlas (TCGA) and Molecular Taxonomy of Breast Cancer International Consortium (METABRIC) were used. The miR-143 high expression group was associated with increased infiltration of anti-cancer immune cells and decreased pro-cancer immune cells, as well as enrichment of the genes relating to T helper (Th1) cells resulting in improved overall survival (OS) in ER-positive breast cancer patients. To the best of our knowledge, this is the first study to demonstrate that high expression of miR-143 in cancer cells associates with a favorable tumor immune microenvironment, upregulation of anti-cancer immune cells, and suppression of the pro-cancer immune cells, associating with better survival of the breast cancer patients.
Cytokine and Growth Factor Delivery from Implanted Platelet-Rich Fibrin Enhances Rabbit Achilles Tendon Healing
Authors: Wong Chin-Chean , Huang Yu-Min , Chen Chih-Hwa , Lin Feng-Huei , Yeh Yi-Yen , Bai Meng-Yi
Published: May 2020

Tendons are hypocellular and hypovascular tissues, and thus, their natural healing capacity is low. In this study, we sought to evaluate the efficacy of platelet-rich fibrin (PRF) to serve as a bioactive scaffold in promoting the healing of rabbit Achilles tendon injury. For in vitro study, the essence portion of PRF was determined through bioluminescent assay. Furthermore, we analyzed the time-sequential cytokines-release kinetics of PRF and evaluated their effects on tenocytes proliferation and tenogenic gene expressions. In animal study, the rabbit Achilles tendon defect was left untreated or implanted with normal/heat-denatured PRF scaffolds. Six weeks postoperatively, the specimens were evaluated through sonographic imaging and histological analysis. The results revealed significantly more activated platelets on bottom half of the PRF scaffold. Cytokine concentrations released from PRF could be detected from the first hour to six days. For the in vitro study, PRF enhanced cell viability and collagen I, collagen III, tenomodulin, and tenascin gene expression compared to the standard culture medium. For in vivo study, sonographic images revealed significantly better tendon healing in the PRF group in terms of tissue echogenicity and homogeneity. The histological analysis showed that the healing tissues in the PRF group had more organized collagen fiber, less vascularity, and minimal cartilage formation. In conclusion, bioactive PRF promotes in vitro tenocytes viability and tenogenic phenotypic differentiation. Administration of a PRF scaffold at the tendon defect promotes tissue healing as evidenced by imaging and histological outcomes.
Bone Marrow Aspirate Concentrate: Its Uses in Osteoarthritis
Authors: Kim Gi Beom , Seo Min-Soo , Park Wook Tae , Lee Gun Woo
Published: May 2020

Human bone marrow (BM) is a kind of source of mesenchymal stem cells (MSCs) as well as growth factors and cytokines that may aid anti-inflammation and regeneration for various tissues, including cartilage and bone. However, since MSCs in BM usually occupy only a small fraction (0.001%) of nucleated cells, bone marrow aspirate concentrate (BMAC) for cartilage pathologies, such as cartilage degeneration, defect, and osteoarthritis, have gained considerable recognition in the last few years due to its potential benefits including disease modifying and regenerative capacity. Although further research with well-designed, randomized, controlled clinical trials is needed to elucidate the exact mechanism of BMAC, this may have the most noteworthy effect in patients with osteoarthritis. The purpose of this article is to review the general characteristics of BMAC, including its constituent, action mechanisms, and related issues. Moreover, this article aims to summarize the clinical outcomes of BMAC reported to date.
Detection of Podocin in Human Urine Sediment Samples by Charge Derivatization and LC-MS-MRM Method
Authors: Bąchor Remigiusz , Gąszczyk Dorota , Panek-Laszczyńska Karolina , Konieczny Andrzej , Witkiewicz Wojciech , Stefanowicz Piotr , Szewczuk Zbigniew
Published: May 2020

Detection of podocytes in urine might serve as a useful diagnostic tool in both primary and secondary glomerular diseases. The utility of podocyturia has been confirmed for both pre-eclampsia and glomerulonephritis. Here, we present a new and sensitive method for qualitative LC-MS-multiple-reaction-monitoring (MRM) analysis of podocin, serving as a podocyturia biomarker in urine sediments. The following podocin tryptic peptides with the 169LQTLEIPFHEIVTK182, 213AVQFLVQTTMK223, 240SIAQDAK246, and 292MIAAEAEK299 sequences were applied as a model. The selective chemical derivatization of the ε amino group of C-terminal lysine residue in tryptic peptides, by 2,4,6-triphenylpyrylium salt (TPP) as a fixed charge tag, was employed to increase the ionization efficiency, in routine ESI-MS analysis. Additionally, the generation of a reporter ion, in the form of a protonated 2,4,6-triphenylpyridinium cation, makes the derivatized peptide analysis in the MRM mode unambiguous. Identification of derivatized and non-derivatized peptides were performed, and the obtained results suggest that the peptide with the 292MIAAEAEK299 sequence may serve as a marker of podocyturia.
Reactions of Plasmodium falciparum Ferredoxin:NADP+ Oxidoreductase with Redox Cycling Xenobiotics: A Mechanistic Study
Authors: Lesanavičius Mindaugas , Aliverti Alessandro , Šarlauskas Jonas , Čėnas Narimantas
Published: May 2020

Ferredoxin:NADP+ oxidoreductase from Plasmodium falciparum (PfFNR) catalyzes the NADPH-dependent reduction of ferredoxin (PfFd), which provides redox equivalents for the biosynthesis of isoprenoids and fatty acids in the apicoplast. Like other flavin-dependent electrontransferases, PfFNR is a potential source of free radicals of quinones and other redox cycling compounds. We report here a kinetic study of the reduction of quinones, nitroaromatic compounds and aromatic N-oxides by PfFNR. We show that all these groups of compounds are reduced in a single-electron pathway, their reactivity increasing with the increase in their single-electron reduction midpoint potential (E17). The reactivity of nitroaromatics is lower than that of quinones and aromatic N-oxides, which is in line with the differences in their electron self-exchange rate constants. Quinone reduction proceeds via a ping-pong mechanism. During the reoxidation of reduced FAD by quinones, the oxidation of FADH. to FAD is the possible rate-limiting step. The calculated electron transfer distances in the reaction of PfFNR with various electron acceptors are similar to those of Anabaena FNR, thus demonstrating their similar “intrinsic” reactivity. Ferredoxin stimulated quinone- and nitro-reductase reactions of PfFNR, evidently providing an additional reduction pathway via reduced PfFd. Based on the available data, PfFNR and possibly PfFd may play a central role in the reductive activation of quinones, nitroaromatics and aromatic N-oxides in P. falciparum, contributing to their antiplasmodial action.
The Impact of Estrogen Receptor in Arterial and Lymphatic Vascular Diseases
Authors: Fontaine Coralie , Morfoisse Florent , Tatin Florence , Zamora Audrey , Zahreddine Rana , Henrion Daniel , Arnal Jean-François , Lenfant Françoise , Garmy-Susini Barbara
Published: May 2020

The lower incidence of cardiovascular diseases in pre-menopausal women compared to men is well-known documented. This protection has been largely attributed to the protective effect of estrogens, which exert many beneficial effects against arterial diseases, including vasodilatation, acceleration of healing in response to arterial injury, arterial collateral growth and atheroprotection. More recently, with the visualization of the lymphatic vessels, the impact of estrogens on lymphedema and lymphatic diseases started to be elucidated. These estrogenic effects are mediated not only by the classic nuclear/genomic actions via the specific estrogen receptor (ER) α and β, but also by rapid extra-nuclear membrane-initiated steroid signaling (MISS). The ERs are expressed by endothelial, lymphatic and smooth muscle cells in the different vessels. In this review, we will summarize the complex vascular effects of estrogens and selective estrogen receptor modulators (SERMs) that have been described using different transgenic mouse models with selective loss of ERα function and numerous animal models of vascular and lymphatic diseases.
Coenzyme Q10 Supplementation Improves Adipokine Levels and Alleviates Inflammation and Lipid Peroxidation in Conditions of Metabolic Syndrome: A Meta-Analysis of Randomized Controlled Trials
Authors: Dludla Phiwayinkosi V. , Orlando Patrick , Silvestri Sonia , Marcheggiani Fabio , Cirilli Ilenia , Nyambuya Tawanda M. , Mxinwa Vuyolwethu , Mokgalaboni Kabelo , Nkambule Bongani B. , Johnson Rabia , Mazibuko-Mbeje Sithandiwe E. , Muller Christo J. F. , Louw Johan , Tiano Luca
Published: May 2020

Evidence from randomized controlled trials (RCTs) suggests that coenzyme Q10 (CoQ10) can regulate adipokine levels to impact inflammation and oxidative stress in conditions of metabolic syndrome. Here, prominent electronic databases such as MEDLINE, Cochrane Library, and EMBASE were searched for eligible RCTs reporting on any correlation between adipokine levels and modulation of inflammation and oxidative stress in individuals with metabolic syndrome taking CoQ10. The risk of bias was assessed using the modified Black and Downs checklist, while the Grading of Recommendations Assessment, Development and Evaluation (GRADE) tool was used to evaluate the quality of evidence. Results from the current meta-analysis, involving 318 participants, showed that CoQ10 supplementation in individuals with metabolic syndrome increased adiponectin levels when compared to those on placebo (SMD: 1.44 [95% CI: −0.13, 3.00]; I2 = 96%, p < 0.00001). Moreover, CoQ10 supplementation significantly lowered inflammation markers in individuals with metabolic syndrome in comparison to those on placebo (SMD: −0.31 [95% CI: −0.54, −0.08]; I2 = 51%, p = 0.07). Such benefits with CoQ10 supplementation were related to its ameliorative effects on lipid peroxidation by reducing malondialdehyde levels, concomitant to improving glucose control and liver function. The overall findings suggest that optimal regulation of adipokine function is crucial for the beneficial effects of CoQ10 in improving metabolic health.
Regulation of Sulfur Homeostasis in Mycorrhizal Maize Plants Grown in a Fe-Limited Environment
Authors: Chorianopoulou Styliani N. , Sigalas Petros P. , Tsoutsoura Niki , Apodiakou Anastasia , Saridis Georgios , Ventouris Yannis E. , Bouranis Dimitris L.
Published: May 2020

Sulfur is an essential macronutrient for growth of higher plants. The entry of the sulfate anion into the plant, its importation into the plastids for assimilation, its long-distance transport through the vasculature, and its storage in the vacuoles require specific sulfate transporter proteins. In this study, mycorrhizal and non-mycorrhizal maize plants were grown for 60 days in an S-deprived substrate, whilst iron was provided to the plants in the sparingly soluble form of FePO4. On day 60, sulfate was provided to the plants. The gene expression patterns of a number of sulfate transporters as well as sulfate assimilation enzymes were studied in leaves and roots of maize plants, both before as well as after sulfate supply. Prolonged sulfur deprivation resulted in a more or less uniform response of the genes’ expressions in the roots of non-mycorrhizal and mycorrhizal plants. This was not the case neither in the roots and leaves after the supply of sulfur, nor in the leaves of the plants during the S-deprived period of time. It is concluded that mycorrhizal symbiosis modified plant demands for reduced sulfur, regulating accordingly the uptake, distribution, and assimilation of the sulfate anion.
The HD-ZIP II Transcription Factors Regulate Plant Architecture through the Auxin Pathway
Authors: He Guanhua , Liu Pan , Zhao Huixian , Sun Jiaqiang
Published: May 2020

The homeodomain-leucine zipper (HD-ZIP) family transcription factors play important roles in plant growth and development. However, the underlying mechanisms remain largely unclear. Here we found that ATHB2, encoding a HD-ZIP transcription factor, is an early auxin responsive gene. Phenotypic analyses show that overexpression of ATHB2 impairs plant architecture, including reduced plant height and small leaves, and also reduces auxin response in leaves when grown in soil. Simultaneously, the seedlings with chemical induction of ATHB2 exhibit abnormal root gravitropism, a typical auxin-related phenotype. We further show that the auxin response pattern is altered in roots of the inducible ATHB2 seedlings. Consistently, the transcript levels of some auxin biosynthetic and transport genes are significantly decreased in these transgenic seedlings. Further, protein and promoter sequence analyses in common wheat showed that the HD-ZIP II subfamily transcription factors have highly conserved motifs and most of these encoding gene promoters contain the canonical auxin-responsive elements. Expression analyses confirm that some of these HD-ZIP II genes are indeed regulated by auxin in wheat. Together, our results suggest that the HD-ZIP II subfamily transcription factors regulate plant development possibly through the auxin pathway in plants.
Chronic Systemic Exposure to Low-Dose Rotenone Induced Central and Peripheral Neuropathology and Motor Deficits in Mice: Reproducible Animal Model of Parkinson’s Disease
Authors: Miyazaki Ikuko , Isooka Nami , Imafuku Fuminori , Sun Jin , Kikuoka Ryo , Furukawa Chieko , Asanuma Masato
Published: May 2020

Epidemiological studies demonstrated that pesticide exposure, such as rotenone and paraquat, increases the risk of Parkinson’s disease (PD). Chronic systemic exposure to rotenone, a mitochondrial complex I inhibitor, could reproduce many features of PD. However, the adoption of the models is limiting because of variability in animal sensitivity and the inability of other investigators to consistently reproduce the PD neuropathology. In addition, most of rotenone models were produced in rats. Here, we tried to establish a high-reproducible rotenone model using C57BL/6J mice. The rotenone mouse model was produced by chronic systemic exposure to a low dose of rotenone (2.5 mg/kg/day) for 4 weeks by subcutaneous implantation of rotenone-filled osmotic mini pump. The rotenone-treated mice exhibited motor deficits assessed by open field, rotarod and cylinder test and gastrointestinal dysfunction. Rotenone treatment decreased the number of dopaminergic neuronal cells in the substantia nigra pars compacta (SNpc) and lesioned nerve terminal in the striatum. In addition, we observed significant reduction of cholinergic neurons in the dorsal motor nucleus of the vagus (DMV) and the intestinal myenteric plexus. Moreover, α-synuclein was accumulated in neuronal soma in the SNpc, DMV and intestinal myenteric plexus in rotenone-treated mice. These data suggest that the low-dose rotenone mouse model could reproduce behavioral and central and peripheral neurodegenerative features of PD and be a useful model for investigation of PD pathogenesis.
Review of Small Gas Turbine Engines and Their Adaptation for Automotive Waste Heat Recovery Systems
Authors: Kozak Dariusz , Mazuro Paweł
Published: May 2020

Current commercial and heavy-duty powertrains are geared towards emissions reduction. Energy recovery from exhaust gases has great potential, considering the mechanical work to be transferred back to the engine. For this purpose, an additional turbine can be implemented behind a turbocharger; this solution is called turbocompounding (TC). This paper considers the adaptation of turbine wheels and gearboxes of small turboshaft and turbojet engines into a two-stage TC system for a six-cylinder opposed-piston engine that is currently under development. The initial conditions are presented in the first section, while a comparison between small turboshaft and turbojet engines and their components for TC is presented in the second section. Based on the comparative study, a total number of 7 turbojet and 8 turboshaft engines were considered for the TC unit.
Selected Aspects of Evaluating Knowledge Management Quality in Contemporary Enterprises
Authors: Sobolewska Olga , Bitkowska Agnieszka
Published: May 2020

The main aim of the article was to estimate select aspects of knowledge management quality evaluations in contemporary enterprises from theoretical and practical perspectives. Measuring knowledge management is the biggest challenge for both theoreticians and practitioners. The survey was addressed to organizations conducting business activity in Poland. The research was carried out in 2019 in the form of an online survey. For international organizations, the survey was intended for representatives of these companies’ local branches. It has been shown that the factors that most strongly affect the quality of knowledge are directly related to infrastructure and information technology systems (IT systems). The article contributes to managerial practice by pointing out the importance of evaluating knowledge management quality from the process perspective. The article’s originality lies in the contribution to the literature of evaluating knowledge management quality by empirically analyzing it in contemporary enterprises. The results of research in the field involving assessing the quality of knowledge management have shown the need to focus not only on information technology tools (IT tools) related to infrastructure, but also on the processes approach, taking into account the priority role of the employees.
Information Dynamics in Complex Systems Negates a Dichotomy between Chance and Necessity
Authors: Weber Georg F.
Published: May 2020

Entropy increases in the execution of linear physical processes. At equilibrium, all uncertainty about the future is removed and information about the past is lost. Complex systems, on the other hand, can lead to the emergence of order, sustain uncertainty about the future, and generate new information to replace all old information about the system in finite time. The Kolmogorov–Sinai entropy for events and the Kolmogorov–Chaitin complexity for strings of numbers both approximate Shannon’s entropy (an indicator for the removal of uncertainty), indicating that information production is equivalent to the degree of complexity of an event. Thus, in the execution of non-linear processes, information entropy is inseparably tied to thermodynamic entropy. Therein, the critical decision points (bifurcations), which can exert lasting impact on the evolution of the future (the “butterfly effect”), defy the definition of being either born from randomness or from determination. Nevertheless, their information evolution and degree of complexity are amenable to measurement and can meaningfully replace the dichotomy of chance versus necessity. Common anthropomorphic perceptions do not accurately account for the transient durability of information, the potential for major consequences by small actions, or the absence of a discernible opposition between coincidence and inevitability.
Research on Uyghur Pattern Matching Based on Syllable Features
Authors: Abliz Wayit , Maimaiti Maihemuti , Wu Hao , Wushouer Jiamila , Abiderexiti Kahaerjiang , Yibulayin Tuergen , Wumaier Aishan
Published: May 2020

Pattern matching is widely used in various fields such as information retrieval, natural language processing (NLP), data mining and network security. In Uyghur (a typical agglutinative, low-resource language with complex morphology, spoken by the ethnic Uyghur group in Xinjiang, China), research on pattern matching is also ongoing. Due to the language characteristics, the pattern matching using characters and words as basic units has insufficient performance. There are two problems for pattern matching: (1) vowel weakening and (2) morphological changes caused by suffixes. In view of the above problems, this paper proposes a Boyer–Moore-U (BM-U) algorithm and a retrievable syllable coding format based on the syllable features of the Uyghur language and the improvement of the Boyer–Moore (BM) algorithm. This algorithm uses syllable features to perform pattern matching, which effectively solves the problem of weakening vowels, and it can better match words with stem shape changes. Finally, in the pattern matching experiments based on character-encoded text and syllable-encoded text for vowel-weakened words, the BM-U algorithm precision, recall, F1-measure and accuracy are improved by 4%, 55%, 33%, 25% and 10%, 52%, 38%, 38% compared to the BM algorithm.
Investigating the Potential of Gamification to Improve Seniors’ Experience and Use of Technology
Authors: Minge Michael , Cymek Dietlind Helene
Published: May 2020

A non-negligible proportion of seniors rarely uses new technology. Far too often, these rather tech unexperienced seniors struggle with new soft- or hard-ware, that do not provide easy access. As a result, some seniors avoid or completely desist to further use “these modern technologies” and consequently miss out on the benefits of digital age. Game-inspired design is supposed to be a promising way to overcome some perceived barriers of seniors by providing hedonic value during early interaction. Previous research has shown that game-inspired design is suitable to motivate seniors’ use of Health-IT. To investigate its potential to facilitate the use of information and communication technology (ICT), an experimental study was conducted. The study investigated the appeal of various gamification features, which were embedded in a prototype of an ICT learning software and compared it to a non-gamified version of the software. Results indicate that the concept of gamified ICT learning software appeals to seniors in general, but that the acceptance of different gamification features is quite diverse. A clear-cut superiority of adding gamification to the software was not found. After interacting with both software versions, seniors in around equal parts preferred either the non-gamified version, the gamified version, or could not decide. Those seniors that clearly favored the gamified version were particularly fond of continuous positive feedback and receiving rewards for each task they have accomplished. Whereas the remaining seniors rather disliked the intensive endorsement through these two features and decided against the gamified version, albeit they liked many of the other game features. Our results underline the necessity of following a user-centered design approach when developing game-inspired applications, and the need for an individualized use of gamification elements to meet the needs of the heterogeneous group of senior users.
Dynamics-Preserving Graph Embedding for Community Mining and Network Immunization
Authors: Zhong Jianan , Qiu Hongjun , Shi Benyun
Published: May 2020

In recent years, the graph embedding approach has drawn a lot of attention in the field of network representation and analytics, the purpose of which is to automatically encode network elements into a low-dimensional vector space by preserving certain structural properties. On this basis, downstream machine learning methods can be implemented to solve static network analytic tasks, for example, node clustering based on community-preserving embeddings. However, by focusing only on structural properties, it would be difficult to characterize and manipulate various dynamics operating on the network. In the field of complex networks, epidemic spreading is one of the most typical dynamics in networks, while network immunization is one of the effective methods to suppress the epidemics. Accordingly, in this paper, we present a dynamics-preserving graph embedding method (EpiEm) to preserve the property of epidemic dynamics on networks, i.e., the infectiousness and vulnerability of network nodes. Specifically, we first generate a set of propagation sequences through simulating the Susceptible-Infectious process on a network. Then, we learn node embeddings from an influence matrix using a singular value decomposition method. Finally, we show that the node embeddings can be used to solve epidemics-related community mining and network immunization problems. The experimental results in real-world networks show that the proposed embedding method outperforms several benchmark methods with respect to both community mining and network immunization. The proposed method offers new insights into the exploration of other collective dynamics in complex networks using the graph embedding approach, such as opinion formation in social networks.
An Application of a Fuzzy TOPSIS Multi-Criteria Decision Analysis Algorithm for Dry Bulk Carrier Selection
Authors: Sahin Bekir , Yip Tsz Leung , Tseng Po-Hsing , Kabak Mehmet , Soylu Ahmet
Published: May 2020

Decision-making in ship investment is a complex and difficult process with trade-offs. The purpose of this study is to guide the decisions on the purchase of dry bulk carriers based on a Fuzzy Technique for Order Preference by Similarity to Ideal Solution (Fuzzy TOPSIS) multi-criteria decision analysis algorithm. A set of criteria and a set of alternatives based on various characteristics are developed and evaluated with expert questionnaires. The proposed method provides weights for a given criteria to provide ranked alternatives, identifying the optimal solution. The alternatives among the multiple suppliers are ranked in the context of the shipping investment. This study reveals that shipowners are rather acting with a holistic perspective in ship purchase; however, we prove a detailed analysis is required.
Evaluation of the Potential of Fungal Biopesticides for the Biological Control of the Seed Bug, Elasmolomus pallens (Dallas) (Hemiptera: Rhyparochromidae)
Authors: Umaru Fredrick Fidelis , Simarani Khanom
Published: May 2020

Elasmolomus pallens is a post-harvest insect pest of groundnuts which causes severe yield loss to farmers, particularly in Africa and Asia. Resistance to synthetic chemicals has been on the rise among insects and is a constraint on insecticides regulations. In view of the drive for alternative approaches to synthetic insecticides, this study evaluated the potential of biopesticides based on entomopathogenic fungi against E. pallens under laboratory conditions. Fungal isolates from the bug cadaver including Fusarium proliferatum F1, Aspergillus tamarii F2, A. flavus F3, Trichoderma atroviride F4, A. niger F4, and Metarhizium anisopliae (Meschn.) Sorokin, originating from the cadaver of Zonocerus variegatus were screened for virulence against the bug. Adult bugs were dipped briefly in conidial concentration 1 × 108 conidial mL−1 and observed at 25 ± 2 °C, 80 ± 10 RH and 14: 10 L:D for 10 days. The fungal isolates caused mortality ranging from 48 to 100% based on their potential to infect and kill the bug. Five conidial concentrations (1 × 104 to 1 × 108 conidia mL−1) were evaluated against adult bugs in the multiple-dose virulence bioassay. Lethal concentrations (LC50 and LC90) values of 6.75 × 106 and 4.42 × 109 conidia mL−1 were obtained for A. flavus F3 while M. anisopliae had 8.0 × 106 and 6.14 × 108 conidia mL−1 respectively. Lethal time (LT50 and LT90) values were 3.3 and 6.2 days for A. flavus F3 compared to 3.6 and 5.6 days for M. anisopliae, respectively. Thus, A. flavus F3 showed potential against E. pallens; and can be considered as an ideal isolate for incorporation into formulations for field applications.
Thermal Tolerance of Fruit-Feeding Butterflies (Lepidoptera: Nymphalidae) in Contrasting Mountaintop Environments
Authors: Silva Vanessa Diniz e , Beirão Marina Vale , Cardoso Danon Clemes
Published: May 2020

Ectothermic organisms, such as insects, are highly temperature dependent and are good models for studies that predict organisms’ responses to global climate change. Predicting how climate change may affect species distributions is a complicated task. However, it is possible to estimate species’ physiological constraints through maximum critical temperature, which may indicate if the species can tolerate new climates. Butterflies are useful organisms for studies of thermal tolerance. We tested if species have different thermal tolerances and if different habitats influence the thermal tolerance of the butterflies present in Brazil’s campo rupestre (open areas) and forest islands (shaded areas). A total of 394 fruit-feeding butterflies, comprising 45 species, were tested. The results separated the species into two statistically different groups: the resistant species with maximum critical temperature of 53.8 ± 7.4 °C, and the non-resistant species with maximum critical temperature of 48.2 ± 7.4 °C. The species of butterflies displayed differences in maximum critical temperature between the campo rupestre and forest islands that can be related to the two distinct habitats, but this did not correlate phylogenetically. Species from the forest islands were also divided into two groups, “resistant” and “non-resistant”, probably due to the heterogeneity of the habitat; the forest islands have a canopy, and in the understory, there are shaded and sunny areas. Species from forest islands, especially species that displayed lower thermal tolerance, may be more susceptible to global warming.
Mitochondrial Functioning ≠ General Intelligence
Authors: Savi Alexander O. , van der Maas Han L. J. , Maris Gunter K. J. , Marsman Maarten
Published: May 2020

Geary puts forward an appealing argument for the consideration of mitochondrial functioning as a candidate for a formative g Geary (2019); it is also an ambitious argument [...]
Statistical Modeling of Bilge Water Discharge from Ships During Normal Operation
Authors: Motoyoshi Munekazu , Nishi Yoshiki
Published: May 2020

This study estimated the amount of bilge water spilled from ships during normal operation and identified the contributing factors of the discharge by building a statistical model. To build the statistical model, we collected as much information as possible about the amount of bilge water in ships, then used the collected information to formulate a probability distribution of the discharge amount according to Bayesian statistics, and determined the parameters included in the model by applying a Markov Chain Monte Carlo method. The analysis of those parameters shows that the integrated bilge treatment system (IBTS) primarily contributes to reductions in the discharge amount, and that the container-type ship is involved with especially large discharge amounts.
The Development of Marine Energy Extraction
Authors: O’Doherty Tim , Mason-Jones Allan
Published: May 2020

Accompanied with an increase in world population there is a growing demand for energy from both the industrial and domestic sectors [...]
Nitrogen Oxides and Particulate Matter from Marine Diesel Oil (MDO), Emulsified MDO, and Dimethyl Ether Fuels in Auxiliary Marine Engines
Authors: Park Jinkyu , Choi Iksoo , Oh Jungmo , Lee Changhee
Published: May 2020

Exhaust gases from ships and automobiles have a significant impact on people and the environment. As a result, diesel engines used in land and marine vehicles are gradually being restricted, and low-carbon engines are under development. This study considers marine diesel oil (MDO) that is used in ships to meet the emission regulations required by the International Maritime Organization. This investigation explores the method and application technology for the reduction of nitrogen oxides (NOx) and particulate matter using emulsified fuel and mass-produced dimethyl ether (DME) fuel, which are analyzed. When comparing emulsified fuel and DME fuel to MDO, which is a ship oil, NOx are reduced by 20–45% and the particulate matter is reduced by 60–97%. When emulsified fuel containing moisture is used, the combustion chamber temperature is lowered due to the optimal expansion by moisture contained in the fuel. The particulate matter is also reduced. When DME fuel is used, it reduces the particulate matter by more than 97% in comparison with the existing MDO fuel and the emulsified fuel. The conditions are believed to be suitable for combustion and they can be satisfied by supplying oxygen during post-combustion.
A Design Parameter for Reef Beach Profiles—A Methodology Applied to Cadiz, Spain
Authors: Contreras Antonio , Muñoz-Perez Juan J. , Contreras Francisco , Gomez-Pina Gregorio , Ruiz-Ortiz Veronica , Chamorro Gabriel , Cabrera Pablo
Published: May 2020

The southwestern coast of Spain is in a tidal zone (mesotidal) which causes the equilibrium profile to be developed in two different sections: the breakage section and the swash section. These two sections give rise to the typical bi-parabolic profile existing in tidal seas. The existence of areas with reefs/rocks which interrupt the normal development of the typical bi-parabolic profile causes different types of beach profiles. The objective of this article is designing an easy methodology for determining new formulations for the design parameters of the equilibrium profile of beaches with reefs in tidal seas. These formulations are applied on 16 profiles to quantify the error between the real profile data and the modelling results. A comparative analysis is extended to the formulations proposed by other authors, from which it is found that better results are obtained with the new formulations.
Patents on Endophytic Fungi Related to Secondary Metabolites and Biotransformation Applications
Authors: Torres-Mendoza Daniel , Ortega Humberto E. , Cubilla-Rios Luis
Published: May 2020

Endophytic fungi are an important group of microorganisms and one of the least studied. They enhance their host’s resistance against abiotic stress, disease, insects, pathogens and mammalian herbivores by producing secondary metabolites with a wide spectrum of biological activity. Therefore, they could be an alternative source of secondary metabolites for applications in medicine, pharmacy and agriculture. In this review, we analyzed patents related to the production of secondary metabolites and biotransformation processes through endophytic fungi and their fields of application. We examined 245 patents (224 related to secondary metabolite production and 21 for biotransformation). The most patented fungi in the development of these applications belong to the Aspergillus, Fusarium, Trichoderma, Penicillium, and Phomopsis genera and cover uses in the biomedicine, agriculture, food, and biotechnology industries.
Selenium Biofortification of Crop Food by Beneficial Microorganisms
Authors: Ye Yuanming , Qu Jingwang , Pu Yao , Rao Shen , Xu Feng , Wu Chu
Published: May 2020

Selenium (Se) is essential for human health, however, Se is deficient in soil in many places all around the world, resulting in human diseases, such as notorious Keshan disease and Keshin–Beck disease. Therefore, Se biofortification is a popular approach to improve Se uptake and maintain human health. Beneficial microorganisms, including mycorrhizal and root endophytic fungi, dark septate fungi, and plant growth-promoting rhizobacteria (PGPRs), show multiple functions, especially increased plant nutrition uptake, growth and yield, and resistance to abiotic stresses. Such functions can be used for Se biofortification and increased growth and yield under drought and salt stress. The present review summarizes the use of mycorrhizal fungi and PGPRs in Se biofortification, aiming to improving their practical use.
Precious Metal Mutual Fund Performance Evaluation: A Series Two-Stage DEA Modeling Approach
Authors: Tsolas Ioannis E.
Published: May 2020

This paper documents a new series two-stage data envelopment analysis (DEA) modeling framework for mutual fund performance evaluation in terms of operational and portfolio management efficiency that is implemented to a sample of precious metal mutual funds (PMMFs). In the first and second stage, one-input/one-output and multi-input/one-output settings are used, respectively. In the light of the results, the funds assessed are inefficient in both operational and portfolio management process and in particular, they seem to be more inefficiently operated. The operational management efficiency is correlated with portfolio management efficiency and, therefore, sample funds should give more emphasis on their operational policies to ensure their success in the industry. The research framework may not only benefit PMMFs, but also funds of other classes to quantify their performance and improve their competitive advantages.
Is Bitcoin Similar to Gold? An Integrated Overview of Empirical Findings
Authors: Kyriazis Nikolaos A.
Published: May 2020

This paper sets out to explore whether Bitcoin can be considered as a globally accepted asset that has a resemblance to gold, which is widely considered to be the safest choice. An integrated overview of the empirical findings generated by the nascent but increasingly proliferating literature concerning the nexus between Bitcoin and gold is provided. The majority of evidence reveals that Bitcoin has a long way to go before it acquires the same characteristics as the safe-haven asset of gold. Overall, Bitcoin is found to be an efficient hedge against oil and stock market indices, but to a lesser extent than gold. Bitcoin presents low or negative correlations or an asymmetric non-linear linkage with gold. Despite sharing some common features with traditional assets, Bitcoin is found to be a good hedging asset in portfolios with gold. Moreover, evidence reveals that gold is a better and more stable safe-haven investment than Bitcoin.
Preparation of a Smart Port Indicator and Calculation of a Ranking for the Spanish Port System
Authors: Rodrigo González Alberto , González-Cancelas Nicoletta , Molina Serrano Beatriz , Orive Alberto
Published: May 2020

Nowadays, the denomination of “smart” is associated with several production and management devices and to logistics and distribution, too. Thus, this qualification is used in different application fields. All of them are different and diverse, but the most important common characteristics of these devices or Smart Systems are based on their novel and more efficient way of working. The smart trend is consolidated in the port environment, too. The ports’ need to become more intelligent is modifying their own operations. Therefore, this paper includes an analysis of Spanish Port System, in order to evaluate its ports, considering the new concept called “Smart Port”. Some indicators and measure variables are defined, to allow for quantifying technical items. These items—both quantitative and qualitative ones—which reflect, in the most objective and complete way possible, the adaptation and achievement of characteristics and objectives associated with the Smart Port concept in all ports were studied, in order to obtain an index of Smart Ports that allows us to calculate the ranking of the Spanish Smart Ports. Considering the analysis of the Smart Port concept and its current and future trends, it has been determined that the development of a port must be based on the digitalization, on use of ICT and on automation of port processes.