bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluating the Accessibility of Urban Public Open Spaces Based on an Improved 2SFCA Model: A Case Study Within Chengdu’s Second Ring Road
Authors: Jian Ling , Xia Xiaojiang , Zhao Yinbing , Zhang Yang , Wang Yuanqiao , Tang Yi , Chang Jie , Wang Changliu
Published: January 2025

The rational allocation of urban public open spaces (UPOS) is critical for creating a livable urban environment. Traditional Two-Step Floating Catchment Area (2SFCA) models often lack sufficient quantitative analysis regarding the supply of urban public service facilities and population demand. This study, taking the area within Chengdu’s Second Ring Road as an example, proposes a 2SFCA model that integrates both supply and demand improvements to evaluate UPOS accessibility. The accessibility results are further analyzed using hotspot analysis, and blind zone detection. In terms of supply improvements, the model incorporates additional indicators beyond the spatial area of UPOS, including service quality and the diversity of surrounding environmental service functions, to better evaluate the overall attractiveness of UPOS to residents. On the demand side, besides population size, the model incorporates the spatial distribution of residents and differences in social characteristics affecting UPOS demand. Results indicate that the improved 2SFCA model, which considers both the attractiveness of UPOS and residents’ demand, significantly enhances the accuracy of accessibility assessments. There are substantial differences in service quality among UPOS, while the diversity of surrounding environmental service functions remains generally high. UPOS demand follows a “high in the northeast—low in the southwest” spatial pattern. The spatial distribution of UPOS accessibility shows a “high in the west—low in the east” pattern, opposite to the demand distribution, indicating a supply–demand mismatch. UPOS accessibility identifies one hotspot cluster and four cold spot clusters, with large areas showing no significant characteristics. Additionally, 10.58% of the study area remains blind zones, requiring urgent attention. This study offers a more scientific method and framework for research on the spatial layout and supply–demand matching of UPOS.
Spatial–Temporal Dynamics of Adventitious Roots of Typha domingensis Pers. Seedlings Grown with Auxin/Cytokinin
Authors: Hernández-Piedra Guadalupe , Ruiz-Carrera Violeta , Sánchez Alberto J. , Escalante-Espinosa Erika , Calva-Calva Graciano
Published: January 2025

The spatial–temporal dynamics of an in vitro radicular system of Typha domingensis for the development of rhizofiltration technologies, with the potential for use as a phytotreatment of eutrophicated water, were studied for the first time in the roots of seedlings and in rhizotron systems. The effect of indole-3-acetic acid (AIA) in combination with kinetin (CIN) or 6-benzylaminopurine (BAP) on seedlings cultivated in the light and dark in three radicular systems and in a rhizotrophic regime for the screening of dynamic rhizogenic lines, by weekly allometric measurements of the length and number of roots, were studied. Inhibition of the elongation and branching velocities of roots by BAP and light was observed but CIN increased elongation and branching. In rhizotrons cultivated in light and dark conditions with different AIA/CIN ratios, isolated root explants remained inactive; however, roots attached to a meristematic base presented a significant increase in growth development, with values comparable to those of roots attached to seedlings cultivated in light without hormones. The results revealed that six adventitious rhizogenic root lines with basal meristems have the potential for use in a wide range of environmental and innovative applications in phytotreatment technologies involving eutrophicated water.
Effect of the Degree of Crystallinity of Base Material and Welded Material on the Mechanical Property of Ultrasonically Welded CF/PA6 Joints
Authors: Shi Ruoya , Li Mingyang , Ao Sansan , Li Yang
Published: January 2025

Ultrasonic welding (USW) is considered one of the most suitable methods to join semi-crystalline carbon fiber-reinforced thermoplastics (CFRTPs). The degree of crystallinity (DoC) of the semi-crystalline resin will affect the ultrasonic welding process by affecting the mechanical properties of the base material. In addition, ultrasonic welding parameters will affect the joint performance by affecting the DoC of the welded material at the welding interface. This paper investigates the effect of DoC of carbon fiber-reinforced PA6 (CF/PA6) base material and welded material on its ultrasonically welded joints’ performance. Distinct pre-welding heat treatments are conducted on the base material before welding. The DoC is calculated by DSC, while the crystalline phases (α and γ phases) and crystallize size are determined using XRD. The results demonstrate that the heat treatment process of heating temperature of 180 °C and cooling with an oven (180-O) could increase the DoC of CF/PA6 from 27.2% to 33% and the ratio of α/γ from 0.38 to 0.75. The joint strength of 180-O sheets reached 17.7 MPa, which is 26.7% higher than that of the as-received sheets. The DoC of the welded material at the welding interface obtained with different combinations of welding parameters is characterized. Higher welding force and amplitude result in faster cooling rate at the welding interface and more effective strain-induced crystallization, leading to higher DoC of the welding interface.
Mechanical Properties and Corrosion Resistance of Thin Ceria and Phosphate Mono- and Multilayers Deposited on Technically Pure Al 1050
Authors: Cherneva Sabina , Andreeva Reni , Stoychev Dimitar
Published: January 2025

Calcium phosphates are often used for biomedical applications. Hydroxyapatite, for example, has a wide range of applications because it mimics the mineral component of natural bone. Widespread interest in the catalytic properties of ceria is due to its use in automotive catalytic converters. Effect of electroless deposited on (non-anodized and anodized) Al 1050 with monolayer Ce2O3 + CeO2, consecutive deposited bilayer Ce2O3 + CeO2/Ca5(PO4)3OH or consecutive deposited bilayer Ce2O3 + CeO2/(AlPO4 + AlOOH + CePO4) systems on the indentation modulus (EIT) and hardness (HIT), as well as their corrosion-protective ability were investigated. For structural, chemical, electrochemical, and mechanical characterization of the investigated systems, the following methods were used: scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDXS), X-ray photoelectron spectroscopy (XPS), polarization resistance (Rp), corrosion rate (CR) analysis, and nanoindentation. It was found that the HIT and EIT of the coatings deposited on an anodized aluminum substrate were much higher than those deposited on a non-anodized aluminum substrate. It established a specific influence of the morphology and chemical composition of formed conversion layers on HIT and EIT and improved the corrosion-protective effect of these layers. The obtained results are valuable since there is no data on the mechanical properties of such coatings in the literature to date.
UHPC Viability for Nuclear Storage Facilities: Synthesis and Critical Review of Durability, Thermal, and Nuclear Properties for Informed Mix Modifications
Authors: Igrashkina Nataliia , Moustafa Mohamed A.
Published: January 2025

Spent nuclear fuel (SNF) from the United States’ nuclear power plants has been placed in dry cask storage systems since the 1980s. Due to the lack of a clear path for permanent geological repository for SNF, consolidated and long-term storage solutions that use durable concrete and avoid current aging and licensing challenges are becoming indispensable. Ultra-high-performance concrete (UHPC) is a rapidly growing advanced concrete solution with superior mechanical and durability properties that can help realize future resilient nuclear storage facilities. Thus, the overall goal of this review study is to demonstrate the viability of UHPC as a long-term solution for future nuclear storage facilities. The paper first identifies all possible non-nuclear (environmental) and nuclear (thermal and radiation-induced) degradation mechanisms in concrete overpacks and storage modules with critical assessment and projections on UHPC performance in comparison to current conventional concrete solutions. Next, since concrete serves as a shielding material in nuclear settings, the preliminary attenuation properties of UHPC from emerging studies are synthesized along with the possible mix modifications to improve its attenuation performance. The paper identifies the major knowledge gaps to inform future research and development, aimed at rethinking the design of SNF dry storage facilities by incorporating UHPC.
Design Techniques for the Optimal Creation of a Robot for Interaction with Children with Autism Spectrum Disorder
Authors: Tamaral Cristofer , Hernandez Lidia , Baltasar Clara , Martin Jose San
Published: January 2025

Educational robotics is a sector that is being integrated into classrooms to achieve innovative and effective learning in the early stages of children’s education. However, it is not only applied in education but has great importance in its use with children with special needs to improve their quality of life. The convenience of robots applied to interaction with children with autism spectrum disorder (ASD) has been widely demonstrated. In this work, a study is carried out on what design patterns a robot focused on children with autism should have. It is necessary to make known the characteristics of this and, from there, how to apply these concepts to the effective design of a device. A robot, TEA-2, is proposed that encompasses all these guidelines in a single robot.
Efficient and Secure Traffic Scheduling Based on Private Sketch
Authors: Chen Yang , Wu Huishu , Ren Xuhao
Published: January 2025

In today’s data–driven world, the explosive growth of network traffic often leads to network congestion, which seriously affects service performance and user experience. Network traffic scheduling is one of the key technologies to deal with congestion problems. Traditional traffic scheduling methods often rely on static rules or pre–defined policies, which make it difficult to cope with dynamically changing network traffic patterns. Additionally, the inability to efficiently manage tail contributors that disproportionately contribute to traffic can further exacerbate congestion issues. In this paper, we propose ESTS, an efficient and secure traffic scheduling based on private sketch, capable of identifying tail contributors to adjust routing and prevent congestion. The key idea is to develop a randomized admission (RA) structure, linking two count–mean–min (CMM) sketches. The first CMM sketch records cold items, while the second, following the RA structure, stores hot items with high frequency. Moreover, considering that tail contributors may leak private information, we incorporate Gaussian noise uniformly into the CMM sketch and RA structure. Experimental evaluations on real and synthetic datasets demonstrate that ESTS significantly improves the accuracy of feature distribution estimation and privacy preservation. Compared to baseline methods, the ESTS framework achieves a 25% reduction in average relative error and a 30% improvement in tail contributor identification accuracy. These results underline the framework’s efficiency and reliability.
Bioactive Molecules from the Invasive Blue Crab Callinectes sapidus Exoskeleton: Evaluation of Reducing, Radical Scavenging, and Antitumor Activities
Authors: Longo Francesco , Attanzio Alessandro , Marretta Laura , Luparello Claudio , Indelicato Serena , Bongiorno David , Barone Giampaolo , Tesoriere Luisa , Giardina Ilenia Concetta , Abruscato Giulia , Perlotti Manuela , Hornsby Lucie Branwen , Arizza Vincenzo , Vazzana Mirella , Vizzini Aiti , Martino Chiara , Listro Angelica , Queiroz Vinicius , Fabbrizio Antonio , Ciaccio Paolo Salvatore Francesco , Cascioferro Stella Maria , Di Gaudio Francesca , Mauro Manuela
Published: January 2025

In recent years, the invasive Atlantic blue crab (Callinectes sapidus) has increased its spread throughout the Mediterranean Sea, threatening native biodiversity and local economies. This study aimed to valorize C. sapidus sampled in Sicily by utilizing its exoskeleton as a source of chitosan, astaxanthin, and bio-phenolic compounds. These biomolecules were evaluated for their reducing, radical scavenging, and antitumor activity. The ferric ion reducing antioxidant power (FRAP) and the free radical scavenging activity against radical 2,2-Diphenyl-1-picrylhydrazyl (DPPH) were significantly higher for chitosan (3.16 ± 0.10 mg AAE/g and 8.1 ± 0.10 µmol TE/g). No significant differences were observed among the tested biomolecules in their activity in scavenging the radical 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Both bio-phenolic compounds and astaxanthin exhibited dose-dependent cytotoxicity on CaCo-2 (IC50 = 12.47 and 18 µg/mL) and HepG2 (IC50 = 10.25 and 1.26 µg/mL) cell lines, while only bio-phenols showed no cytotoxic effect on differentiated CaCo-2 cells up to 20 µg/mL. These findings highlight the value of blue crab by-products in supporting a circular economy, offering a sustainable approach to managing this invasive species while providing bioactive compounds with promising medical and nutraceutical applications.
Isolation of Bacillus paralichenifromis BL-1 and Its Potential Application in Producing Bioflocculants Using Phenol Saline Wastewater
Authors: Zhang Tao , Guo Rongkai , Liu Fanshu , Zhang Lei , Li Linxiao , Zhang Rongfei , Shao Chaogang , Zhou Junbo , Ding Fan , Yu Lan
Published: January 2025

Phenolic compounds are harmful organic pollutants found in wastewater from the chemical and pharmaceutical industries, which are frequently accompanied by high saline concentrations. Microorganism-based biodegradation represents an environmentally friendly and cost-effective strategy for phenol removal. In this study, we isolated a bioflocculant-producing Bacillus paralicheniformis BL-1 that is capable of phenol degradation in high-salinity conditions. Differential gene expression analysis revealed the down-regulation of genes related to the synthesis of extracellular polymeric substances and the up-regulation of poly-γ-glutamate biosynthesis in 10% NaCl conditions. These findings indicate that poly-γ-glutamate is the main large biomolecule produced by B. paralicheniformis BL-1. A further investigation suggested that salinity stress resulted in the down-regulated expression of the genes involved in iron homeostasis. Therefore, alleviating iron limitation by supplying excess iron could improve cell growth and, thus, increase the phenol removal rate and flocculating activity. The productivity of poly-γ-glutamate reached 2.23 g/L, and the phenol removal rate reached 73.83% in the synthetic medium supplemented with 10% NaCl, 500 mg/L phenol, and 250 μM FeCl3.
Beneficial Effects of In Vitro Reconstructed Human Gut Microbiota by Ginseng Extract Fermentation on Intestinal Cell Lines
Authors: Finazzi Margherita , Bovio Federica , Forcella Matilde , Lasagni Marina , Fusi Paola , Di Gennaro Patrizia
Published: January 2025

Oxidative stress caused by reactive oxygen species (ROS) affects the aging process and increases the likelihood of several diseases. A new frontier in its prevention includes bioactive foods and natural extracts that can be introduced by the diet in combination with specific probiotics. Among the natural compounds that we can introduce by the diet, Panax ginseng extract is one of the most utilized since it contains a vast number of bioactive molecules such as phenolic acids, flavonoids, and polysaccharides that have been shown to possess antioxidant, anti-ageing, anti-cancer, and immunomodulatory activity. In this work, the ability of a P. ginseng extract in combination with a probiotic formulation was taken into consideration to evaluate its effects on the modulation of in vitro reconstructed human gut microbiota (HGM). After evaluating the growth of the individual strains on the ginseng extract, we tested the in vitro reconstructed HGM setup (probiotics, minimal core, and whole community) using 2% w/v ginseng as the only carbon and energy source. The probiotic strains reached the highest growth, while the minimal core and the whole community showed almost the same growth. Specifically, the presence of the ginseng extract favors L. plantarum and B. animalis subsp. lactis among the probiotics, while B. cellulosilyticus prevails over the other strains in the minimal core condition. In the presence of both probiotics and minimal core strains, L. plantarum, B. animalis subsp. lactis, and B. cellulosilyticus reach the highest growth values. The bacterial metabolites produced during ginseng extract fermentation in the three conditions were administered to human intestinal epithelial cells (HT-29) to investigate a potential antioxidant effect. Remarkably, our results highlighted a significant reduction in the total ROS and a slightly reduction in the cytosolic superoxide anion content in HT-29 cells treated with bacterial metabolites deriving from ginseng extract fermentation by the whole community.
Environmental Applications of Mass Spectrometry for Emerging Contaminants
Authors: Meher Anil Kumar , Zarouri Akli
Published: January 2025

Emerging contaminants (ECs), encompassing pharmaceuticals, personal care products, pesticides, and industrial chemicals, represent a growing threat to ecosystems and human health due to their persistence, bioaccumulation potential, and often-unknown toxicological profiles. Addressing these challenges necessitates advanced analytical tools capable of detecting and quantifying trace levels of ECs in complex environmental matrices. This review highlights the pivotal role of mass spectrometry (MS) in monitoring ECs, emphasizing its high sensitivity, specificity, and versatility across various techniques such as Gas Chromatography-Mass Spectrometry (GC-MS), Liquid Chromatography-Mass Spectrometry (LC-MS), and High-Resolution Mass Spectrometry (HR-MS). The application of MS has facilitated the real-time detection of volatile organic compounds, the comprehensive non-targeted screening of unknown contaminants, and accurate quantification in diverse matrices including water, soil, and air. Despite its effectiveness, challenges such as matrix interferences, a lack of standardized methodologies, and limited spectral libraries persist. However, recent advancements, including hybrid MS systems and the integration of artificial intelligence (AI), are paving the way for more efficient environmental monitoring and predictive modeling of contaminant behavior. Continued innovation in MS technologies and collaborative efforts are essential to overcome existing challenges and ensure sustainable solutions for mitigating the risks associated with emerging contaminants.
Antiviral Activity and Underlying Mechanism of Moslae herba Aqueous Extract for Treating SARS-CoV-2
Authors: Feng Yan , Ge Qiong , Gao Jian , Wu Zhuoying , Zhang Yunyi , Mao Haiyan , Wu Beibei , Xu Changping
Published: January 2025

Despite the widespread use of COVID-19 vaccines, there is still a global need to find effective therapeutics to deal with the variants of SARS-CoV-2. Moslae herba (MH) is a herbal medicine credited with antiviral effects. This study aims to investigate the antiviral effects and the underlying mechanism of aqueous extract of Moslae herba (AEMH) for treating SARS-CoV-2. The in vitro anti-SARS-CoV-2 activity of AEMH was evaluated using cell viability and viral load. Component analysis was performed by HPLC-ESI-Q-TOF/MS. The connection between COVID-19 and AEMH was constructed by integrating network pharmacology and transcriptome profiles to seek the core targets. The components with antiviral activities were analyzed by molecular docking and in vitro pharmacological verification. AEMH exerted anti-SARS-CoV-2 effects by inhibiting viral replication and reducing cell death caused by infection (IC50 is 170 μg/mL for omicron strain). A total of 27 components were identified from AEMH. Through matching 119 intersection targets of ‘disease and drug’ with 1082 differentially expressed genes of COVID-19 patients, nine genes were screened. Of the nine, the PNP and TPI1 were identified as core targets as AEMH treatment significantly regulated the mRNA expression level of the two genes on infected cells. Three components, caffeic acid, luteolin, and rosmarinic acid, displayed antiviral activities in verification. Molecular docking also demonstrated they could form stable bonds with the core targets. This study explored the antiviral activity and possible mechanism of AEMH for treating SARS-CoV-2, which could provide basic data and reference for the clinical application of MH.
Mononuclear Aluminum–Fluoride Ions, AlFx(+/−)—Study of Plausible Frameworks of Complexes with Biomolecules and Their In Vitro Toxicity
Authors: Pavlovič Anja , Janžič Larisa , Sršen Lucija , Kopitar Andreja Nataša , Edwards Kathleen F. , Liebman Joel F. , Ponikvar-Svet Maja
Published: January 2025

The importance of fluorine and aluminum in all aspects of daily life has led to an enormous increase in human exposure to these elements in their various forms. It is therefore important to understand the routes of exposure and to investigate and understand the potential toxicity. Of particular concern are aluminum–fluoride complexes (AlFx), which are able to mimic the natural isostructural phosphate group and influence the activity of numerous essential phosphoryl transferases. Our review of salts of ionic AlFx species, which plausibly form the framework of complexes with biomolecules, revealed that the octahedral configuration of aluminum in the active site of the enzyme is preferred over the trigonal-bipyramidal structure. The effects of varying concentrations of fluoride, aluminum and AlFx—from micromolar to millimolar levels—on the viability and apoptosis rate of THP-1 monocytes were investigated using phosphate buffer solution as a culture media to simulate physiological conditions. Our results suggest that aluminum can reduce the direct toxicity of fluoride through the formation of AlFx. In view of the results found, further in vitro studies are required to clarify the toxicity mechanisms of these species.
4D-Printed Magnetic Responsive Bilayer Hydrogel
Authors: Li Yangyang , Li Yuanyi , Cao Jiawei , Luo Peng , Liu Jianpeng , Ma Lina , Gao Guo-Lin , Jiang Zaixing
Published: January 2025

Despite its widespread application in targeted drug delivery, soft robotics, and smart screens, magnetic hydrogel still faces challenges from lagging mechanical performance to sluggish response times. In this paper, a methodology of in situ generation of magnetic hydrogel based on 3D printing of poly-N-isopropylacrylamide (PNIPAM) is presented. A temperature-responsive PNIPAM hydrogel was prepared by 3D printing, and Fe2O3 magnetic particles were generated in situ within the PNIPAM network to generate the magnetic hydrogel. By forming uniformly distributed magnetic particles in situ within the polymer network, 3D printing of customized magnetic hydrogel materials was successfully achieved. The bilayer hydrogel structure was designed according to the different swelling ratios of temperature-sensitive hydrogel and magnetic hydrogel. Combined with the excellent mechanical properties of PNIPAM and printable magnetic hydrogel, 4D-printed remote magnetic field triggered shape morphing of bilayers of five-petal flower-shaped hydrogels was presented, and the deformation process was finished within 300 s.
In Situ X-Ray Study During Thermal Cycle Treatment Combined with Complementary Ex Situ Investigation of InGaN Quantum Wells
Authors: Grzanka Ewa , Bauer Sondes , Lachowski Artur , Grzanka Szymon , Czernecki Robert , So Byeongchan , Baumbach Tilo , Leszczyński Mike
Published: January 2025

In situ X-ray reciprocal space mapping was performed during the interval heating and cooling of InGaN/GaN quantum wells (QWs) grown via metal–organic vapor phase epitaxy (MOVPE). Our detailed in situ X-ray analysis enabled us to track changes in the peak intensities and radial and angular broadenings of the reflection. By simulating the radial diffraction profiles recorded during the thermal cycle treatment, we demonstrate the presence of indium concentration distributions (ICDs) in the different QWs of the heterostructure (1. QW, bottom, 2. QW, middle, and 3. QW, upper). During the heating process, we found that the homogenization of the QWs occurred in the temperature range of 850 °C to 920 °C, manifesting in a reduction in ICDs in the QWs. Furthermore, there is a critical temperature (T = 940 °C) at which the mean value of the indium concentration starts to decrease below 15% in 1. QW, indicating the initiation of decomposition in 1. QW. Moreover, further heating up to 1000 °C results in extended diffuse scattering along the angular direction of the diffraction spot, confirming the propagation of the decomposition and the formation of trapezoidal objects, which contain voids and amorphous materials (In-Ga). Heating InGaN QWs up to T = 1000 °C led to a simultaneous decrease in the indium content and ICDs. During the cooling phase, there was no significant variation in the indium concentrations in the different QWs but rather an increase in the defect area, which contributes to the amplification of diffuse scattering. A comparison of ex situ complementary high-resolution transmission microscopy (Ex-HRTEM) measurements performed at room temperature before and after the thermal cycle treatment provides proof of the formation of four different types of defects in the QWs, which result from the decomposition of 1. QW during the heating phase. This, in turn, has strongly influenced the intensity of the photoluminescence emission spectra without any detectable shift in the emission wavelength λMQWs.
Egg Consumption and Mortality: A Prospective Cohort Study of Australian Community-Dwelling Older Adults
Authors: Wild Holly , Gasevic Danijela , Woods Robyn L. , Ryan Joanne , Wolfe Rory , Chen Yuquan , Govindaraju Thara , McNeil John J. , McCaffrey Tracy , Beilin Lawrence J. , Ilic Dragan , Owen Alice J.
Published: January 2025

Background/Objectives: Egg consumption in adults has been linked with a modestly increased risk of all-cause and CVD mortality. However, evidence on adults aged 65 y+ is limited. The objective of this study was to investigate the association between egg intake and mortality in community-dwelling older adults. Methods: In this prospective cohort study, 8756 adults aged 70+ years, participants in the ASPirin in Reducing Events in the Elderly (ASPREE) Longitudinal Study of Older Persons, self-reported the frequency of their total egg intake: never/infrequently (rarely/never, 1–2 times/month), weekly (1–6 times/week), and daily (daily/several times per day). All-cause and cause-specific (cardiovascular disease [CVD] and cancer) mortality was established from at least two sources: medical records, death notices, next of kin, or death registry linkage. The association between egg intake and mortality was assessed using Cox proportional hazards regression analysis, adjusted for socio-demographic, health-related, and clinical factors and overall dietary quality. Results: Over the median 5.9-year follow-up period, a total of 1034 all-cause deaths (11.8%) were documented. A 29% lower risk of CVD mortality (HR (95% CI): 0.71 [0.54–0.92]) and a 17% (HR (95% CI): 0.83 [0.71–0.96]) lower risk of all-cause mortality were observed among those who consumed eggs weekly, compared to those who consumed eggs never/infrequently; no statistically significant association was observed for weekly consumption and cancer mortality. In contrast, compared to those that never or infrequently consumed eggs, daily consumption had slightly higher odds of mortality, though these results did not reach statistical significance. Conclusions: The consumption of eggs 1–6 times per week was associated with a lower risk of all-cause mortality and CVD mortality in community-dwelling adults aged 70 years and over. These findings may be important to inform the development of evidence-based guidelines for egg consumption.
Nutritional Status of 8,128,014 Chilean and Immigrant Children and Adolescents Evaluated by the National Board of School Aid and Scholarships (JUNAEB) Between 2013 and 2023
Authors: Bustos-Arriagada Edson , Vásquez Fabián , Etchegaray-Armijo Karina , López-Arana Sandra
Published: January 2025

Introduction: Nutritional issues, including overweight and obesity, along with the rising number of immigrants facing their own nutritional problems, continue to keep Chile on alert. Objective: To evaluate the epidemiological and nutritional status changes among Chilean and immigrant boys, girls, and adolescents (BGA) in schools evaluated by the National Board of School Aid and Scholarships (JUNAEB) from 2013 to 2023. Methods: This descriptive study analyzed individual, anonymous, and de-identified data on the nutritional status of BGA in pre-kindergarten, kindergarten, first grade, fifth grade, and the first year of high school using the JUNAEB Nutritional Map. Results: The sample consisted of 8,128,014 BGA, 49.2% women and 50.8% men. In 2013, immigrant BGA represented 0.4% of the total number of children evaluated, and by 2023, this percentage increased to 7.9%. It was observed that Chilean BGA had a lower proportion of thinness, risk of thinness, stunting, and normal weight, but a higher proportion of overnutrition compared to immigrants, similar to observations made during the COVID-19 pandemic. Comparisons by sex showed that Chilean and immigrant women had a lower percentage of thinness, risk of thinness, obesity, severe obesity, and stunting, and a higher proportion of normal weight when compared to men. Conclusions: The rise in immigrant BGA within the Chilean school system, together with the nutritional differences between both groups, highlights the need to consider these particularities when designing nutritional public policies in the health and education sectors.
Translation and Validation of the Italian Version of the Team-Based Learning Student Assessment Instrument (TBL-SAI) in Nursing Students
Authors: Vannini Valeria , Caruso Rosario , Alberti Sara , Rovesti Sergio , Ferri Paola
Published: January 2025

Background/Objectives: Team-based learning is an educational strategy that promotes active learning and student engagement through structured team activities. It contrasts with traditional teaching models by emphasizing student preparation and collaboration. The TBL-SAI is a reliable and valid instrument designed to evaluate students’ attitudes towards TBL, assessing dimensions such as accountability, preference for lecture or team-based learning, and satisfaction with TBL. Validating the TBL-SAI in different languages ensures its applicability and accuracy in diverse educational contexts, supporting the global adoption of TBL methodologies. Thus far, no Italian validation of this tool has been performed. The aim of this study is to validate the Italian version of the Team-Based Learning Student Assessment Instrument (TBL-SAI) among nursing students. Methods: A methodological-driven translation process and a cross-sectional study design were used. The study was conducted from November 2022 to March 2023 and involved 202 nursing students from the University of Modena and Reggio Emilia (Italy). Convenience sampling was employed to recruit participants who had experienced at least one TBL session during the academic year 2022/2023. The validation process included translation, back-translation, and expert panel review, followed by a pilot test to ensure clarity and comprehension. Data were collected using the self-administered TBL-SAI. The responses were analyzed using Exploratory Structural Equation Modeling (ESEM) to assess the scale’s validity and McDonald’s ω to evaluate internal consistency. Results: The ESEM results supported the validity of the Italian TBL-SAI, maintaining the factor structure proposed in the original instrument. The model fit indices indicated a good fit (χ2(318) = 384.097, p = 0.0065; RMSEA = 0.032; CFI = 0.974; TLI = 0.957). McDonald’s ω values exceeded 0.70 for all factors, confirming adequate internal consistency. Conclusions: This study successfully validated the Italian version of the TBL-SAI, demonstrating its reliability and validity for assessing perceptions of TBL among Italian undergraduate nursing students. However, future studies should employ Confirmatory Factor Analysis to further test the proposed factor structure and explore the instrument’s applicability in various educational settings. The validated TBL-SAI is recommended for use in evaluating students’ attitudes towards TBL, providing actionable feedback for educators to improve teaching methods and integrate TBL methodologies effectively.
Polymer-Free Electrospinning of β-Cyclodextrin–Oligolactide for Magnolol and Honokiol Pharmaceutical Formulations
Authors: Blaj Diana-Andreea , Peptu Catalina A. , Balan-Porcarasu Mihaela , Peptu Cristian , Tuchilus Cristina Gabriela , Ochiuz Lacramioara
Published: January 2025

Background: Magnolol (MG) and honokiol (HK) are bioactive compounds extracted from Magnolia obovata and Magnolia Officinalis trees with significant pharmacological properties, including antioxidant and antibacterial activity. However, their poor water solubility and low bioavailability limit the therapeutic potential. Methods: To address these limitations, this study aims to develop MG and HK formulations by co-electrospinning using custom-synthesized β-cyclodextrin–oligolactide (β-CDLA) derivatives. MALDI MS and NMR were employed for the structural assessment of the β-CDLA derivatives. This polymer-free electrospinning technique utilizes the high solubility of β-CDLA to incorporate MG and HK into fibrous webs. The morphology of the resulting fibers is established by SEM and further characterized using FTIR and NMR spectroscopy to confirm the successful incorporation of MG and HK. The antioxidant activity was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, while the antimicrobial activity was evaluated against several standard microorganisms (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans). Results: The MG and HK electrospun formulations were prepared using highly concentrated feed solutions in dimethylformamide (180% w/v). The resulting β-CDLA fibers, with diameters above 400 nm and an active compound content of 7% wt., exhibited enhanced long-term antioxidant activity and improved antimicrobial efficacy, including notable activity against Escherichia coli. Conclusions: This study demonstrates the potential of MG and HK-loaded β-CDLA fibrous formulations as delivery systems with prolonged antioxidant activity and notable antibacterial efficacy, providing a promising platform for biomedical applications.
Photodynamic Therapy Effects with Curcuma longa L. Active Ingredients in Gel and Blue LED on Acne: A Randomized, Controlled, and Double-Blind Clinical Study
Authors: Crusca Jaqueline de Souza , de Moraes Luis Henrique Oliveira , Figueira Thiago Gomes , Parizotto Nivaldo Antonio , Rodrigues Gerson Jhonatan
Published: January 2025

Photodynamic therapy (PDT) using the photosensitizer curcumin and blue light has a relevant effect on bacteriological decontamination caused by C. acne. The aim is to verify PDT’s effectiveness with curcumin in individuals diagnosed with moderate to severe acne. This study was carried out on a total of 35 volunteers of both genders (12–32 years old), with moderate to severe acne vulgaris. The volunteers were randomized into five groups: L (LED), V (Vehicle), C (Curcumin), L + V (LED + Vehicle), and L + C (LED + Curcumin). The curcumin gel and LED with blue wavelength (450 nm ± 10 nm) were used. Qualitative and quantitative evaluations were used to verify the efficacy of the treatment by counting inflamed and non-inflamed lesions. The L + C group until day 30 showed a lower percentage of inflammatory lesions than the Vehicle group for the same period. On day 60, the L + C group showed lower inflammatory lesion values compared to the other groups. Intragroup analysis of hydration in the Vehicle group (V) showed a difference on days 30 and 60 compared to day zero. In an intragroup analysis, the L + C group showed a decrease in the mean scores on day 30, and day 60 compared to day zero, showing an improvement in the psychosocial status of these volunteers. Taken together, our results showed that the combination of blue LED therapy and curcumin proved to be an effective and safe treatment for reducing inflamed acne lesions in individuals with moderate to severe acne, while also enhancing their quality of life from a psychosocial perspective.
Silicon Effects Depend upon Insect Herbivore Guild and Has Limited Influence on Gall-Inducing Insects of Bauhinia brevipes
Authors: Demetrio Guilherme Ramos , Venâncio Henrique , Batista Janaina Correa , Santos Jean Carlos
Published: January 2025

Silicon (Si) is a widely recognized element in plant defense, often enhancing resistance to herbivory by strengthening cell walls and deterring feeding by external herbivores. However, its impact on internal, endophytic herbivores, such as gall-inducing insects, remains underexplored. This study investigates the role of silicon in Bauhinia brevipes, focusing on its effects on herbivory by insects. We hypothesize that while silicon strengthens plant tissues and reduces feeding by external herbivores, it may have a limited effect on internal feeders, such as gall-inducing insects. Our results indicate that silicon accumulation in leaves significantly reduces herbivory by chewing insects but has no direct effect on the occurrence of gall-inducing insects. Silicon content in galled tissues was lower compared to healthy leaves, suggesting that gall-inducing insects may manipulate silicon distribution to mitigate its defensive effects. Our results indicate that hypersensitivity reactions were positively influenced by silicon, highlighting the role of this element in enhancing localized defense mechanisms. Our findings reveal silicon’s tissue-specific roles in plant defense, emphasizing the need for more research on its nuanced interactions with endophytic herbivores and implications for ecological applications. This research contributes to the literature on silicon’s multifaceted role in plant–herbivore interactions and its potential applications in sustainable pest management.
Seasonal Pattern of Endo-β-Mannanase Activity During Germination of Jeffersonia dubia, Exhibiting Morphophysiological Dormancy
Authors: Kwon Young Hyun , Lee Seung Youn , Rhie Yong Ha
Published: January 2025

Morphophysiological dormancy (MPD) is considered one of the most primitive dormancy classes among seed plants. While extensive studies have examined the occurrence of endo-β-mannanase in seeds with physiological dormancy (PD) or non-dormancy, little is known about the activity of this enzyme in seeds with MPD. This study aimed to investigate the temporal and spatial patterns of endo-β-mannanase activity during dormancy break and germination. The research focused on Jeffersonia dubia, a species with deep simple MPD, by monitoring its morphological and biochemical characteristics under natural field conditions. Seeds were buried in the field and exhumed monthly over a year. Key parameters measured included germination, embryo elongation, endosperm weakening, and endo-β-mannanase activity in the exhumed seeds. Scanning electron microscopy was employed to observe structural changes in the endosperm. For the first three months after burial in May, endo-β-mannanase activity was undetectable, and the underdeveloped embryo exhibited minimal elongation. Starting in September, the embryo began to grow, accompanied by increased endo-β-mannanase activity in the micropylar endosperm. Erosion of the endosperm cell wall was observed in the lateral regions surrounding the embryo, whereas the micropylar endosperm showed no obvious signs of collapse or damage. The increase in enzyme activity coincided with moderate temperatures and a corresponding increase in embryo length. During the winter months, embryo elongation ceased for 2–3 months, and enzyme activity declined. However, as germination resumed in early March, enzyme activity increased again. This was followed by micropylar endosperm rupture and the completion of germination. The seasonal pattern of endo-β-mannanase activity observed in seeds with deep simple MPD was distinct from that of seeds with PD, MD, or non-deep MPD, highlighting the unique mechanisms underlying dormancy break and germination in J. dubia.
Syntrichia laevipila Brid., a Bryophyta from Northwest Argentina as a Source of Antioxidants and Antimicrobials
Authors: Jiménez Luis Ignacio , Correa Uriburu Florencia Maria , Martínez Chamás José Javier , Suárez Guillermo Martin , Zampini Iris Catiana , Simirgiotis Mario J. , Isla María Inés
Published: January 2025

In recent years, numerous studies have emerged on the biological activities of bryophytes and their potential for therapeutic use. However, mosses appear to be a relatively overlooked group. The objective of this study was to conduct a phytochemical analysis of one hydroalcoholic extract of Syntrichia laevipila and to evaluate its potential as an antioxidant and antimicrobial agent. The moss was collected in the Chaco Serrano region of Argentina, specifically on Jacaranda mimosifolia, and subsequently extracted by maceration in ethanol/water. UHPLC/ESI/MS/MS analysis identified 32 peaks, including phenolic compounds (phenolic acids, lignans, chalcones, and flavonoids) and non-hydrophilic compounds (terpenoids, fatty acids, and brassinosteroids). Maslinic and oleanolic acids, two triterpenoids present in S. laevipila, were also detected in J. mimosifolia, a substrate of this moss. The concentration of phenolic compounds was 19.05 ± 0.21 µg GAE/mL, while the total flavonoid concentration was 13.13 ± 0.33 µg QE/mL. The determination of reducing and total sugars yielded 0.22 ± 0.03 mg GE/mL and 1.26 ± 0.24 mg GE/mL, respectively, while the concentration of soluble proteins was 90.60 ± 4.50 µg BSAE/mL. The extract exhibited antioxidant properties by scavenging ABTS•+, H2O2, AAPH, and HO• radicals. Additionally, it demonstrated antibacterial activity by inhibiting the growth of four strains of Staphylococcus aureus. The data obtained suggest that the hydroalcoholic extract of S. laevipila possesses significant potential as a natural antioxidant and antimicrobial agent, making it a promising candidate for the development of phytotherapeutic and cosmetic products.
Antioomycete Nanoformulation for Biocontrol of English Walnut Crown and Root Rot Caused by Phytophthora cinnamomi
Authors: Salinas Aldo , Montenegro Iván , Olguín Yusser , Riquelme Natalia , Castillo-Novales Diyanira , Larach Alejandra , Alvarado Laureano , Bravo Guillermo , Madrid Alejandro , Álvaro Juan E. , Besoain Ximena
Published: January 2025

In Chile and worldwide, walnut (Juglans regia L.) production faces significant losses due to crown and root rot caused by the phytopathogen Phytophthora cinnamomi. Currently, control methods have proven insufficient or unfavorable for the environment, increasing the need for sustainable alternatives. This research evaluates nanoemulsions based on extracts of medicinal plants endemic to Chile to control P. cinnamomi in walnut crops. The methodology included an in vitro test to determine the effective inhibitory concentrations of three nanoemulsions (N80, N90, and N100) on the mycelial growth of the phytopathogen, a test on walnut plants under controlled conditions, and two field tests using concentrations between 300 and 500 ppm. The in vitro results showed that the nanoemulsions could inhibit 90% of mycelial growth at 80 to 100 ppm concentrations. In the field, the N90 nanoemulsion at 500 ppm significantly reduced disease symptoms preventively and post-inoculation, compared with the control. This research is the first to study the use of nanoemulsions from native Chilean plants to control P. cinnamomi, showing potential to reduce the use of synthetic fungicides, contributing to safer and more ecological phytosanitary management.
Pequi Pulp (Caryocar brasiliense) Oil-Loaded Emulsions as Cosmetic Products for Topical Use
Authors: Pereira Tácio Fragoso , Borchardt Huelinton , Wanderley Wvandson F. , Vasconcelos Ulrich , Leite Itamara F.
Published: January 2025

The pequi (Caryocar brasiliense) is a typical fruit from the Brazilian Cerrado. From it, pequi pulp oil is extracted, a valuable product for cosmetic applications due to its high levels of unsaturated fatty acids and carotenoids. Carotenoids are antioxidant compounds that are easily oxidized. To improve pulp stability, emulsification techniques with carboxymethylcellulose at 1% (w/w) were used to encapsulate the pequi pulp oil at 1, 3, 5% (w/w), and 8% (w/w) of polysorbate 80® using a high-rotation mechanical stirrer. The pequi pulp oil was first characterized by FTIR and GC-MS. The results indicated the presence of chemical groups characteristic of the oil itself and the presence of a large proportion of fatty acids, which are essential for the maintenance of cutaneous hydration and the barrier, also acting in the tissue repair process. All emulsions presented stable over 120 days with slightly acidic pH values and were compatible with human skin. The droplet diameter was less than 330 nm, and the polydispersity index was around 0.3, indicating systems with low polydispersity. The Zeta potential (ζ) exhibited negative values sufficient to stabilize the emulsified systems. All emulsions behaved as non-Newtonian fluids, presenting pseudo-plastic and thixotropic properties that are considered important for topical applications.
Research Progress on Quantum Dot-Embedded Polymer Films and Plates for LCD Backlight Display
Authors: Xu Bin , Zhou Jiankang , Zhang Chengran , Chang Yunfu , Deng Zhengtao
Published: January 2025

Abstract: Quantum dot–polymer composites have the advantages of high luminescent quantum yield (PLQY), narrow emission half-peak full width (FWHM), and tunable emission spectra, and have broad application prospects in display and lighting fields. Research on quantum dots embedded in polymer films and plates has made great progress in both synthesis technology and optical properties. However, due to the shortcomings of quantum dots, such as cadmium selenide (CdSe), indium phosphide (InP), lead halide perovskite (LHP), poor water, oxygen, and light stability, and incapacity for large-scale synthesis, their practical application is still restricted. Various polymers, such as methyl methacrylate (PMMA), polyethylene terephthalate (PET), polystyrene (PS), polyvinylidene fluoride (PVDF), polypropylene (PP), etc., are widely used in packaging quantum dot materials because of their high plasticity, simple curing, high chemical stability, and good compatibility with quantum dot materials. This paper focuses on the application and development of quantum dot–polymer materials in the field of backlight displays, summarizes and expounds the synthesis strategies, advantages, and disadvantages of different quantum dot–polymer materials, provides inspiration for the optimization of quantum dot–polymer materials, and promotes their application in the field of wide-color-gamut backlight display.