bluuebunny/crossref_metadata_2025 huggingface dataset:
ANALISIS KEPUASAN JAMINAN KESEHATAN NASIONAL PADA PENGGUNA BPJS KESEHATAN DI KOTA SEMARANG
Authors: Listiyana Ita , Rustiana Eunike Raffy
Published: September 2017

National health insurance (NHI) as a part of national social security system (NSIS) is a form of government commitment to the implementation of health insurance among people in Indonesia entirely. State-owned enterprises (SOEs) assigned specifically by the government to provide health care insurance was the BPJS for Health (social security provider for health). The aim of this study was to analyze the satisfaction of national health insurance among BPJS for Health participants in Semarang City based on education, tuition classes, and place of service. This study was analytical survey with cross sectional approach. Sample of Non PBI (Contribution Assistance Recipients) BPJS for Health were 269 respondents, with purposive proportional sampling technique. There was a significant difference regarding the satisfaction of health insurance based on education level (basic, secondary, and high) and place of service (public hospital and private hospital). There was no significant difference on satisfaction of health insurance based on tuition classes (class I, II, and III). BPJS for Health participants who did not satisfied with the national health insurance were 64.7%. The most important complaint was the process of obtaining an inpatient room.
Manajemen Sumber Daya Terhadap Mutu Pelayanan Neonatus Di Puskesmas PONED Oesao Kupang
Authors: Fai Isterina Felandina , Pandie David B.W , Ludji Ina Debora Ratu
Published: September 2017

Terdapat 9 kasus kematian neonatus di tahun 2015 di Puskesmas Oesao.Ditinjau dari aspek sumber daya manusia, aspek  peralatan, aspek obataspek bangunan, aspek SOP belum memenuhi syarat. Penelitian ini bertujuan untuk menganalisis pengaruh manajemen sumber daya secara parsial dan simultan terhadap mutu pelayanan neonatus.Jenis penelitian yang digunakan dalam penelitian ini adalahObservasional Analitik desain cross sectional. Lokasi penelitian di Puskesmas Oesao Oesao Kabupaten Kupang. Populasi dalam penelitian ini adalah semua ibu yang memiliki neonatus di wilayah kerja Puskesmas Oesao dari bulan Januari sampai September 2015 sebanyak 396 orang ibu, teknik pengambilan sampel menggunakan teknik sampling acak sederhana dan sampel penelitian sebanyak 67 ibu yang memiliki neonatus. Hasil analisis univariat menunjukkan bahwa aspek SDM, spek peralatan, aspek obat, aspek bangunan, aspek SOP dan mutu pelayanan neonatus termasuk dalam kategori baik. Hasil Analisis bivariat menunjukkan bahwa secara parsial ada pengaruh antara aspek SDM (p value = 0,004), aspek obat(p value = 0,004) terhadap mutu pelayanan neonatus. Hasil analisis multivariat menunjukkan bahwa secara simultan ada pengaruh antara Aspek SDM (p value = 0,004) dan aspek obat (p value = 0,004) terhadap mutu pelayanan neonatus.

Public Health Center Oesao was considered under perform in term of aspect of human resources, aspects of equipment, aspects of medicine, aspects of the building, and on procedural aspects. At same time, there were 9 cases of neonatal deaths in 2015. This study aimed to analyze the influence of resource management partially and simultaneously on the quality of neonatal care. This type of research used in this study was observational analytical cross-sectional design. The population in this study are all mothers who had newborns in PHC Oesao from January to September 2015 as many as 396 people a mother, a technique of sampling using simple random sampling and samples are 67 mothers with newborns. Results of univariate analysis showed that the human resources aspect, spec equipment, medicine aspects, aspects of the building, the quality aspects of the draft SOP neonatal care included in both categories. The results of the bivariate analysis showed that partial no influence between aspects of HR (p=0.004), Aspects of Medicines (p=0.004) on the quality of Neonatal Care. Multivariate analysis showed that simultaneously there is influence between the human resources aspect (p=0.004) and Aspects of Medicine (p=0.004) on the Quality Neonatal Services. It was recommendet to further enhance regular supervision to improve the quality, quantity, resource management and quality neonatal care.
KOMUNIKASI ORANGTUA-REMAJA DAN PENDIDIKAN ORANGTUA DENGAN PERILAKU SEKSUAL BERISIKO PADA REMAJA
Authors: Gustina Erni
Published: September 2017

Pacaran dan perilaku seksual berkaitan erat satu sama lain. Pacaran akan menghadapkan remaja pada kondisi yang meningkatkan pengalaman seksual. Dilaporkan bahwa perilaku berciuman (48% remaja laki-laki, 30% remaja perempuan), petting (30% remaja laki-laki, 6% remaja perempuan). Tujuan penelitian untuk mengetahui hubungan komunikasi orangtua-remaja, pendidikan orangtua dengan perilaku seksual berisiko pada remaja Tahun 2015. Penelitian ini adalah observasional analitik dengan rancangan cross sectional. Besar sampel adalah 88 orang. Teknik sampling  adalah total sampling. Analisa data menggunakan chi square dan regresi logistik. Hasil penelitian didapatkan perilaku seksual remaja risiko rendah (91,2%), komunikasi orangtua-remaja baik (57,2%). Ada hubungan komunikasi orangtua-remaja dengan perilaku seksual berisiko (p=0,03), ada hubungan pendidikan orangtua dengan perilaku seksual berisiko (p=0,04). Simpulan penelitian ada hubungan komunikasi orangtua-remaja, pendidikan orangtua dengan perilaku seksual berisiko dan komunikasi orangtua-remaja merupakan variabel dominan berhubungan dengan perilaku seksual berisiko pada remaja.

 

Dating and sexual behaviors are closely related to one another. Dating will confront adolescents in conditions that increased the sexual experience. Reported that the behavior of kissing (48% boys, 30% girls), petting (30% boys, 6% girl). The aimed of research to determine the relationship parent-adolescent communication, education of parents with risky sexual behavior in adolescents Year 2015. This study was observational analytic with cross sectional design. The sample size are 88 people. Sampling technique was total sampling. Data were analyzed using chi square and logistic regression. The result showed a low risk of adolescent sexual behavior (91.2%), parent-adolescent communication is good (57.2%). There was a relathionship parent-adolescent communication with risky sexual behavior (p=0.03), there was relationship with the parent education risky sexual behavior (p=0.04). The conclusions of research there was a relationship parent-adolescent communication, education of parents with risky sexual behavior and parent-teenager communication is the dominant variable associated with risky sexual behavior in adolescents.
PERILAKU KONSUMSI MINUMAN RINGAN (SOFTDRINK) DAN PH SALIVA DENGAN KEJADIAN KARIES GIGI
Authors: Fitriati Nur , Hernawan Andri Dwi , Trisnawati Elly
Published: September 2017

Abstrak

Karies gigi adalah penyakit dengan sejumlah faktor risiko yang ditandai dengan demineralisasi pada gigi. Minuman ringan merupakan faktor ekstrinsik kejadian karies gigi. Data Riskesdas (2013) menunjukkan prevalensi penduduk Indonesia yang bermasalah dengan penyakit gigi dan mulut sebanyak 25,9% dan prevalensi provinsi Kalimantan Barat adalah 20,6%. Prevalensi karies gigi umur 10-14 tahun menurut Profil Kesehatan Kota Pontianak meningkat dari 26,9% (2014) menjadi 30,1% (2015). Tujuan penelitian ini untuk mengetahui hubungan antara konsumsi minuman ringan dengan pH saliva dan kejadian karies gigi pada siswa kelas V SD Binaan UPTD Kecamatan Pontianak Timur Tahun 2016.

Penelitian ini menggunakan desain cross sectionaldengan jumlah sampel 159 orang. Hasil penelitian menunjukkan terdapat hubungan yang bermakna pada variabel frekuensi konsumsi (p value=0,001), waktukonsumsi (p value=0,008), cara konsumsi (p value=0,000), perilaku setelah konsumsi (p value=0,000) dengan pH saliva dan adanya hubungan bermakna antara pH saliva (p value=0,000), angka OHIS (p value=0,022) dengan kejadian karies gigi. 

Abstract

Dental caries is disease with a number of risk factors characterized by deminineralization of the tooth. Softdrinks are extrinsic factors on the incidence of dental caries. Riskesdas (2013) indicating that prevalence of West Kalimantan province was 20,6%. The prevalence of dental caries in the 10-14 years age according to Pontianak City Health Profile increased from 26,9% (2014) to 30,1% (2015). The objective of the research is to determine the the relationship between the consumption of softdrinks with pH saliva and the incidence of dental caries in primary school students of class V Patronage UPTD Eastern District of Pontianak 2016.

The research is using cross sectional design with a sample of 159 people. The results is obtained a significant relationship to the variable frequency of consumption (p value = 0,001), time consumption (p value = 0,008), how consumption (p value = 0,000), behavior after consumption (p value = 0,000) with pH saliva and the relationship between pH saliva (p value = 0,000), number OHIS (p value = 0,022) with the incidence of dental caries.
PERAN PETUGAS GIZI DALAM MEMBERIKAN PELAYANAN ASUHAN GIZI PADA PASIEN RAWAT INAP
Authors: Sulistiyanto Anggara Dwi , Handayani Oktia Woro Kasmini , Rustiana Eunike R
Published: September 2017

Abstrak 

 

Pelayanan gizi rumah sakit merupakan bagian yang sangat vital dari sistem pelayanan paripurna terhadap pasien di rumah sakit.Pelayanan gizi diberikan agar pasien mencapai kondisi yang optimal dalam memenuhi kebutuhan giziataupun mengoreksi kelainan metabolisme. Penelitian ini mengenai bagaimana peran petugas gizi dalam memberikan pelayanan asuhan gizi kepada pasien rawat inap di RSUD dr. R. Soetrasno Kabupaten Rembang. Jenis penelitian adalah penelitian observasional dengan pendekatan kualitatif yang mendekripsikan objek dengan mengamati, melakukan wawancara serta mencatat bagaimana petugas gizi memberikan pelayanan asuhan gizi. Data sampel diambil secara purposive sampling dengan jumlah responden 7 orang. Petugas gizi sudah melaksanakan dengan baik sesuai dengan perannya yaitu melakukan monitoring dan evaluasi pasien terhadap dampak asuhan giziserta melaporkan hasil asuhan gizi kepada dokter atau tenaga kesehatan lain. Perlu pembentukan tim terapi gizi tersandart dengan melibatkan tenaga kesehatan lain agar pelayanan gizi rumah sakit lebih optimal.

 

 

Abstract

The hospital nutrition care service is a vital part of the plenary service system to the patients in the hospital. Nutrition care services given to patients in order to achieve optimal conditions in fulfillment of nutritional needs or correcting metabolic abnormalities.This study was conducted to answer whether the role of nutritionists in providing nutrition care services to inpatients in dr. R. Soetrasno hospital, is in accordance with hospital nutrition care service or not.This study was an observational study using qualitative approach, that described object by observing, interviewing and making notes about how is the role of nutritionists. Data was taken using purposive sampling with 7 respondances.Nutritionists already performed their duties properly by doing evaluation to the inpatients and report the results of nutrition care services to the doctor or other health professionals. Hospitals need to create a qualified nutritional therapy team, involving other health professionals.
FAKTOR - FAKTOR YANG BERHUBUNGAN DENGAN PRAKTIK PENEMUAN PNEUMONIA BALITA OLEH BIDAN
Authors: Prabawa Hendra Eka , Azinar Muhammad
Published: September 2017

ABSTRAK

 

Pneumonia merupakan pembunuh utama balita di dunia yang kurang mendapat perhatian bahkan terlupakan (“the forgotten killer”). Cakupan angka penemuan pneumonia balita di Indonesia tahun 2014 masih belum mencapai target yaitu 29,74% (target 80%). Rendahnya cakupan penemuan pneumonia balita disebabkan antara lain karena deteksi kasus oleh bidan sebagai pemberi pelayanan balita sakit di puskesmas masih rendah. Penelitian ini bertujuan untuk mengetahui faktor – faktor yang berhubungan dengan praktik penemuan pneumonia balita oleh bidan di puskesmas wilayah Kabupaten Pemalang. Metode penelitian ini berjenis explanatory research, menggunakan pendekatan cross sectional, dengan populasi penelitian adalah bidan yang bertugas di ruang rawat jalan KIA puskesmas di Kabupaten Pemalang berjumlah 242 orang. Teknik sampling secara purposive didapat 44 responden. Analisis data secara univariat dan bivariat dengan uji chi square. Hasil penelitian didapatkan faktor yang berhubungan dengan praktik penemuan pneumonia oleh bidan adalah pengetahuan (p=0,000), tugas rangkap (p=0,019), lama memegang program (p=0,007) dan dukungan kepala puskemas (p=0,000). Faktor yang tidak berhubungan adalah umur (p=1,000), sikap (p=0,773), ketersediaan fasilitas (p=0,117) dan dukungan mitra kerja (p=1,000).

 

ABSTRACT

 

               Pneumonia is the main killer of children under five in the world who received less attention even forgotten ("the forgotten killer"). Coverage under five pneumonia detection rate in Indonesia in 2014 has yet to reach the target of 29.74% (target 80%). The low coverage of infant pneumonia is caused partly because the detection of cases by midwives as the service provider sick children in health centers is still low. This study aimed to identify factors related to the practice of the invention toddler pneumonia by midwives in health centers Pemalang district. This research method was explanatory research using cross sectional approach, the study population are midwife who served in the room KIA clinics in Pemalang amounted to 242 people. Purposive sampling technique obtained 44 respondents. Data analysis of univariate and bivariate with chi square test. The result showed that factors associated with the practice of the invention of pneumonia by midwives are knowledge (p = 0.000), double duty (p = 0.019), long held the program (p = 0.007) and the head support health centers (p = 0.000). Factors unrelated are age (p = 1.000), attitude (p = 0.773), the availability of facilities (p = 0.117) and the support of partners (p = 1.000).
PENGARUH PEMBERIAN BEDONG TERHADAP PERKEMBANGAN MOTORIK BAYI USIA 3 BULAN
Authors: Solikah Siti Nur , Suminar Saka
Published: September 2017

Pemberian bedong sampai saat ini sudah menjadi tradisi pada masyarakat Indonesia khususnya di Jawa tengah. Sampai saat ini belum diketahui manfaat pentingnya pemberian bedong secara ilmiah tapi justru dapat menghambat perkembangan motorik karena sejak bayi lahir hingga usia tertentubayi tidak mendapatkan kesempatan bergerak bebas dan tidak mendapat stimulasi gerak dari lingkungan.Penelitian ini bertujuan untuk mengetahui pengaruhpemberian bedong terhadap perkembangan motorik pada bayi usia 3 bulan di Desa Cemani Kecamatan Grogol Kabupaten Sukoharjo.Jenis penelitian deskriptif komparatif dengan rancangan penelitian cross sectional. Populasi penelitian ini adalah bayi usia3 bulan di Desa Cemani sebanyak 28.  Pengambilan sampel dilakukan dengan tehnik total sampling, jadi semua populasi diambil sebagai sampel penelitian. Instrumen penelitian menggunakan wawancara dan alat DDST. Uji hipotesis yang digunakan adalah uji t test independent.Analisis uji t test independent menunjukkan hasil signifikansi 0,000 (p < 0,05), maka Ho ditolak sehingga terdapat pengaruhpemberian bedong terhadap perkembangan motorik pada bayi usia 3 bulan di Desa Cemani Kecamatan Grogol Kabupaten Sukoharjo. Semakin lama bayi dibedong maka perkembangan motoriknya semakin suspeck, hal ini ditunjukkan dari nilai t -6,232.

 

Kata kunci: bedong, perkembangan motorik, bayi.

 

Abstract

 

The growth and physical development of a baby is considered as a golden age, a precious stage that influence for his or her entire life. Assessment of growth and development needs to be done to find whether a child's growth in normal range or not. Physical growth and achievement of ability occur quickly during the first year, one of which is motoric development. Infant motor development is influenced by several factors, one of which is culture such as baby wrapping for the newborn.This study aims to determine the effect of bedong on motor development in infants aged 3 months in the Village Cemani Grogol District Sukoharjo District. Using comparative descriptive research with cross sectional study design to all the infant age 3 months in Cemani 28 villages. The research instrument used interviews and DDST (Denver Development Screening Test) tools. Hypothesis test used is independent t test. Conclusion: Independent test t test analysis showed the result of significance 0.000 (p <0,05), hence Ho was rejected so that there was influence of giving of bedong to motor development at baby age 3 month in Cemani Village Grogol District Sukoharjo Regency. The longer the baby wrapped then more suspect motor development delayed, this is shown from the value of t -6.232.

 

Key Words : wrapping, motoric development, newborn
ID:4002 Customizing cancer immunotherapies to match the intrinsic tumor microenvironment
Authors: Nelson Brad
Published: September 2017

Tumor-infiltrating lymphocytes (TIL) are associated with survival in virtually every human cancer studied, but the mechanisms by which they confer protective immunity remain incompletely understood. Focusing on ovarian cancer, our group applies genomic and molecular pathology approaches to define the mechanisms by which the immune system responds to the evolving tumor genome over space and time. We have shown that optimal anti-tumor immunity involves interactions between T cells and antibody-producing B cells and plasma cells in the tumor microenvironment. Moreover, we have identified three patterns of TIL response in ovarian cancer, each carrying distinct implications for immunotherapy. We are translating these insights into clinical trials involving adoptive transfer of tumor-reactive T cells.
ID:3006 RNA Therapeutics and Anabolic Gene Delivery for Tissue Regeneration
Authors: Hu Yu-Chen
Published: September 2017

Regenerative medicine requires coordinated functions of cells, materials and appropriate signaling. Recent years have witnessed the marriage of regenerative medicine and gene delivery by which various genes encoding anabolic/catabolic proteins or RNA therapeutics are delivered into cells to potentiate the tissue regeneration.  This presentation will focus on the use of viral vectors for genetic modification of mesenchymal stem cells derived from bone marrow or adipose tissue for tissue regeneration. In particular, emphasis is placed on the applications of baculovirus, an emerging nonpathogenic gene delivery vector, for the delivery of various anabolic genes and miRNA mimics/sponges to repair tissues
ID3010 Wnt antagonism in cancer stem cells- targeting stemness of cancers via the Wnt-BETA catenin pathway
Authors: Warrier Sudha
Published: September 2017

Cancer stem cells (CSCs) are hypothesized to be the pathological counterpart of normal somatic tissue stem cells. The CSC model proposes that tumours are hierarchically  organised with a subset of tumour cells at their apex, which possess self-renewal and multilineage differentiation potential. Cancer stem cells, unlike the bulk of the cells within the tumor, are elusive to drug treatment and are unaffected on chemo and radiotherapy. These self-renewing cells are responsible for the flare up of cancer and remission long after treatment. Cancer stem cells have a capacity for unlimited self-renewal, as well as the ability to initiate and drive tumor progression in an animal model.

 Activated Wnt/β-Catenin signaling is a key feature of epithelial cancers and is critical for metastasis and epithelial-mesenchymal transition (EMT) , a signature trait of CSCs. We explored the effect of the Wnt antagonist, secreted frizzled related protein 4 (sFRP4) in CSCs in gliomas. We found that sFRP4 chemo-sensitizes GSC-enriched cells to commonly used drugs, by the reversal of EMT and by decreasing drug effluxers. sFRP4 acts at multiple levels of the Wnt- β-Catenin and the Wnt- calcium pathways in inhibiting CSCs. We also identified a novel mechanism of action of sFRP4. These findings could be exploited for designing better targeted strategies to improve chemo-response and eventually eliminate glioblastoma CSCs.
ID2010 Cytotoxicity of aqueous and ethanolic bark extracts of Pithecellobium dulce against human carcinoma cells
Authors: Jalique Shella Mae
Published: September 2017

Cancer cases continue to increase and kill humans. In this paper, we report a study based on anticancer properties of the aqueous and ethanolic  bark extracts of Pithecellobium dulce. Anticancer activities were assayed with standard MTT colorimetric procedure against three human cancer cell lines namely breast  (MCF-7), colon (HCT-116), and hepatocellular (HepG2) in different concentrations. The aqueous extract of the plant revealed the highest toxicity on hepatocellular carcinoma (HepG2) cancer cell line with cell viability of 1.71 percent. On the other hand, its ethanol extracts has the highest toxicity on colon carcinoma (HCT-116) with a percent viability of 6.05 percent.  Based on the results, the bark of the plant can be used to prepare anticancer drug with proper standardization methods
ID2016 Human mesenchymal stem cell derived model of Alzheimer’s disease – effect of Wnt antagonism in neurodegeneration
Authors: Patil Manasi
Published: September 2017

Alzheimer’s disease (AD), one of the most common types of dementia, is a serious and increasing health problem. This progressive disorder is manifested by loss of memory which worsens over time. Although several causative mechanisms for AD has been identified, the specific molecular mechanisms for the onset and progression of the disease has not yet been elucidated. This is mainly because of the lack of patient tissue at various stages of disease, in order to test and ascertain the molecular progression of AD. To address this lacuna, we have developed a human stem cell model of AD, derived from mesenchymal stem cells (MSCs) of perinatal tissues. We first derived cells of the central nervous system (CNS) from MSCs and then subjected them to AD specific neurodegeneration. Onset of neurodegeneration was ascertained by accumulation of BACE, nAChAR and presenilin. Neuronal generation was first established for expression neuronal specific markers such as nestin, Tuj1, Pax6, Sox1 and 2 by immunocytochemistry and quantitative RT-PCR. In the AD model thus established, we analysed further for the role of Wnt antagonists, sFRP3 and 4 and deciphered a link between inhibition of the Wnt-b catenin pathway and the onset of neurodegeneration. These studies would pave the way for the development of specific biomarkers for progression of AD and novel drug targets based on these biomarkers
ID2019 Functional analysis of human type 2 diabetic adipose tissue-derived mesenchymal stem cells
Authors: Trinh Thuy Nhu
Published: September 2017

Background: Stem cell therapy has recently shown promise in the prevention of diabetic complications due to its regenerative potential. The possible applications of human diabetic adipose tissue-derived mesenchymal stem cells (dAT-MSCs) in cell therapy are limited because their characteristics are still not fully understood. Aims: This study aimed to characterize dAT-MSCs in vitro and to investigate the potential application of dAT-MSCs in wound healing. Materials and Methods: dAT-MSCs were characterized under normoxic and hypoxic conditions in vitro and evaluated wound healing capacity in the ischemic flap mouse model. Results: Early growth response factor-1 (EGR-1) and its target genes were highly expressed in dAT-MSCs in comparison to nAT-MSCs, resulting in increasing of genes and protein associated with cell adhesion, insulin resistance, and impaired wound healing. Interestingly, under hypoxic conditions, hypoxia-inducible factor-1α (HIF-1α) can bind to the EGR-1 promoter in dAT-MSCs, but not in nAT-MSCs. The effects of EGR-1 were inhibited by shEGR-1 and PD98059. Mice injected with shEGR-1- dAT-MSCs were improved their wound healing capacity. Furthermore, we found that human nAT-MSC-derived microvesicles (nMVs) could improve dAT-MSC function by altering miRNA and mRNA expressions, which enhanced their migration ability in vitro and wound healing capacity in the ischemic flap mouse model. Conclusion: Our study suggests that dAT-MSCs may contribute to delay wound healing. Interrupting the expression of EGR-1 in dAT-MSCs or transfecting nMVs to dAT-MSCs may be a useful treatment for chronic wounds in diabetic patients.
ID:2041 Animal Component Free Medium Retains the Stability of Hypervariable Region 1 (HV1) of Mitochondrial DNA of Human Full Term Amniotic Fluid Mesenchymal Stem Cells
Authors: Rawi Rohayu Izanwati Mohd
Published: September 2017

Therapeutic potential of human amniotic fluid (hAF) cells in treating human disease is very promising. Furthermore it provides an alternative in isolating stem cells from an accessible source, where the fluid is merely discarded, thus circumventing the ethical concerns. Mesenchymal stem cell (MSC) is one of the heterogenous cell populations in the amniotic fluid (AF), however, its role and potential for stem cell therapy are yet to be discovered. One of the criteria in establishing the clinical grade of MSCs is the ability of the cells to retain their genomic stability in vitro prior to bedside applications. The microenviroment niche of the culture medium used is important to generate the safe MSCs. As mitochondria play the vital role in metabolic task such as apoptosis and cell proliferation, therefore investigating mitochondria DNA (mtDNA) is essential to mark the stability of stem cell. In this study, we aimed to detect variations of mtDNA across the passage number of cultured human amniotic fluid MSCs (hAFMSCs), cultured in animal component free (ACF) medium, specialised for human MSCs. Polymorphisms of hypervariable region 1 (HV1) in the D-loop of mtDNA were investigated in a series of cultured hAFMSCs (passage 1 and 3) from three different samples (three biological replicates) as well as in their respective fresh samples (control). Extracted DNA samples were subjected to PCR to detect the sequence variations of HV1 (nt16024-16365). The sequences were then analysed using SeqScape v2.5 by comparing the sequences with The Cambridge Reference Sequence (rCRS) for human mtDNA. The common C-to-T transition at position 16223 (C16223T) for all three samples was identified. Another common base transition was also identified for the other two samples at position 16311 (T16311C).

However, one of the samples showed more variants; a C-to-T transition at position 16290 (C16290T), an A-to-G at position 16317 (A16317G) and a G-to-A at position 16319 (G16319A). All variants were identified within the sequence of around 341bp nucleotides and their polymorphisms frequency was consistent for all three samples regardless of their passage numbers when compared to control. This pattern of polymorphisms demonstrates the HV1 mtDNA integrity of hAFMSCs cultured in ACF medium, hence highly suggesting the ability of ACF medium in retaining the stability of stem cells mtDNA in vitro. The ACF medium may serve as a good medium in culturing hAFMSCs that is safe and stable for basic research in stem cell and therapeutic applications.
ID:2051 Generation of Microtumors Using 3D Hanging drop culture system and breast cancer cell MCF7 for testing anticancer drug response
Authors: Nguyen Lam-Huyen
Published: September 2017

Cancer is one of the most leading causes of death all over the world. Great deal of effort has been made to find out new agents for cancer treatment. Cancer cell models for drug testing play a critical role in this process. In recent years, 3D cell models were proved to be more effective mimic structure and character of cancer cells in tumors than 2D cell culture, hence they present the effect of drug more accurate to in vivo result. Vietnam which is a tropical country has tremendous potential in cancer drug screening due to its diversity of herbals. However, 3D cancer cell model for testing toxicity of extracts and compounds still be a new concept. The study aimed to generate multicellular tumor spheroid (MCTS) of MCF7 cells using hanging drop technique, which is evaluated as a simple method to create separated spheroids, easy to controlled the size of spheroids and enable to apply to high-throughput screening system. By tracking the spheroid size, growth curve and the exhibition of necrotic core using PI staining, the results indicated that the number of 2500 cells/well gives the most suitable spheroids for anticancer drug screening with the diameter about 500µm and exhibit necrotic core in the 3rd day of seeding. Toxicity of Doxorubicine and Tirapazamine were tested on the model compared which MCF7 cells cultured in 2D condition. The results showed that IC50 of Dox on 3D MCF7 cells was nearly 50 times higher than monolayer MCF7 cells. By contrast, TPZ, the agent demonstrated to have strong toxicity in hypoxia condition, showed the stronger anticancer ability on 3D models than 2D (IC50 of TPZ on 3D models showed significantly lower versus 2D). These results confirmed the promising of MCF7 cells MCTS generated by hanging drop for drug testing.
ID: 1017 Extracellular vesicles of ETV2 transfected fibroblasts stimulate endothelial cells and improve neovascularization in a murine model of hindlimb ischemia
Authors: Pham Phuc Van , Vu Ngoc Bich , Dao Thuy Thi-Thanh , Le Ha Thi-Ngan , Phi Lan Thi , Huynh Oanh Thuy , Truong Mai Thi-Hoang , Nguyen Oanh Thi-Kieu , Phan Ngoc Kim
Published: September 2017

Ischemia are common conditions related to lack of blood supply to tissues. Depending on the ischemic sites, ischemia can cause different diseases, such as hindlimb ischemia, heart infarction and stroke. This study aims to evaluate how extracellular vesicles (EVs) derived from ETV2 transfected fibroblasts affect endothelial cell proliferation and neovascularization in a murine model of hindlimb ischemia. Human fibroblasts were isolated and cultured under standard conditions and expanded to the 3th passage before use in experiments. Human fibroblasts were transduced with a viral vector containing the ETV2 gene. Transduced cells were selected by puromycin treatment. These cells were further cultured for collection of EVs, which were isolated from culture supernatant. Following co-culture with endothelial cells, EVs were evaluated for their effect on endothelial cell proliferation and were directly injected into ischemic tissues of a murine model of hindlimb ischemia. The results showed that EVs could induce endothelial cell proliferation in vitro and improved neovascularization in a murine model of hindlimb ischemia. Our results suggest that EVs derived from ETV2-transfected fibroblasts can be promising non-cellular products for the regeneration of blood vessels.
ID: 1036 FAM134B, a new player in human colorectal cancer pathogenesis
Authors: Islam Farhadul , Gopalan Vinod , Lam Alfred K-Y
Published: September 2017

Background: FAM134B (family with sequence similarity 134, member B) is a relatively new player in many human diseases including cancers. In colorectal carcinomas, FAM134B plays important role in the pathogenesis and associated with biological aggressiveness of the disease. However, expression pattern, the frequency of mutations, methylation status in colon cancer cells and tissue samples has never been studied. Also, the functional roles of FAM134B in cell have never been studied in colorectal cancer.

Objectives: To investigate FAM134B promoter methylation, mutations in tissues samples from patients with colorectal cancer and cell lines. Also, promoter methylation, expression and functional roles of FAM134B in colon cancer were studied.

 Methods: Methylation and mutations in FAM134B sequence in cancer tissues (n=126) and matched non-cancer samples was studied by high-resolution melt curve analysis followed by Sanger sequencing. FAM134B expression was studied and quantified in cell lines and cancer tissues samples using immunofluorescence, immunocytochemistry, Western blot and real time PCR. In vitro functional assays and mouse xenotransplantation model were performed to unveil the molecular roles of FAM134B in colon cancer pathogenesis followed by shRNA-mediated silencing in cells.

 Results: We noted that 46.5% (41/88) patients with colorectal cancer were identified as FAM134B mutations positive. Thirty-one novel pathogenic mutations were detected and these mutations were associated with gender of the patients, presence of metachronous cancer, size, T staging, presence of distant metastases and positivity of microsatellite instability (MSI) in the cancer (p < 0.05). Majority of cancer tissues had shown promoter hyper-methylation and were correlated with reduced mRNA and protein expression in both cancer samples and cells. FAM134B expression in cancer cells derived from advanced stages (stage III; SW48 and stage IV; HCT116) of colon cancer was significantly (p<0.01) reduced when compared to non-neoplastic colon cells (FHC) and cancer cells derived from stage II colon cancer (SW480). Expression of FAM134B mRNA in cancer tissues was noted significantly (p<0.001) downregulated when compared to that of non-cancer tissues samples. FAM134B suppression significantly (p<0.05) increased the proliferation of colon cancer cells, remarkably increased (3452% ; p<0.05) the clonogenic, migration capacity, and increases the proportion of cells in S phase of cell cycle (p<0.01). Xenotransplantation model showed that larger and higher grade tumors were formed in mice treated with FAM134B knockdown cells.

Conclusion: Reduced expression in cancer samples, in vitro and in vivo functional studies implied that FAM134B acts as a cancer inhibitor in colon cancer play important roles in colorectal carcinogenesis.
ID: 1038 Hydrothermal synthesis of carbon nanodots from millets for cancer cells imaging
Authors: Khoa Quang Ngo , Thuy Tran Thi Xuan , Ha Che Thi Cam
Published: September 2017

We presented a green, simple and economical method to synthesize carbon nanodots (C-dots) from millets using hydrothermal synthesis route. The obtained C-dots have average diameter ranging from 6 to 8 nm. Optical measurements showed the formation of hydroxyl, carbonyl/carboxyl, amino functional groups on the particle surfaces, resulting in their high hydrophilicity and bioconjugation. After treatment with C-dots, human cervical and lung cancer cells became bright and exhibited multicolor fluorescence under different excitation wavelength. The achievement demonstrated potential applications of fluorescent C-dots in the field of biomedical application, especially in diagnostic disease techniques.
ID: 1074 The efficiency of adipose-derived stem cell pretreated with hepatocyte growth factor and platelet rich plasma on a liver cirrhosis mouse model
Authors: Nguyen Yen Thi-Kim , Nguyen Nam Hai , Le Trinh Van , Vo Ngoc Hong , Dang Thanh Minh , Do Huy Quang , Truong Nhung Hai
Published: September 2017

Background: Because of their ease of isolation, high proliferation capacity in vitro, as well as their ability to differentiate into liver cells, Adipose-derived stem cell (ADSCs), are considered to be a promised candidate for liver disease treatment, including liver cirrhosis treatment. Recent studies show that hepatocyte growth factor (HGF) can stimulate ADSC to migrate to the injured areas and platelet rich plasma (PRP) can increase the stemness of ADSCs.

Method: In this study, we cultured ADSCs in the medium supplemented 20 ng/ml and 10% PRP; then transplanted them into the cirrhosis mouse model. After 11 weeks of CCl4 induction (1ml/kg, three times per week), mice were divided into 3 groups: (1) PBS group which were injected 0.2 ml PBS; (2) ADSC group which were transplanted 5x105 ADSCs non-pretreated with HGF and PRP; (3) ADSC/HGF+PRP group which were transplanted 5x105 ADSCs pretreated with HGF and PRP via the tail vein. We evaluated the effectiveness of the therapeutic at the day 7th and 14th after transplantation.

Results: The results show that the transplantation of ADSC pretreated with HGF and PRP after seven days improves the body weight (increase 4.673%); decreases the ALT level (p < 0.05), the total leukocyte number (p < 0.05) and the expression of Pro-collagen (decrease 4.1 times) as well as α-SMA (decrease 5.1 times), in comparison to the ADSC group. ADSCs pretreated with HGF and PRP also help to improve the liver tissue structure.

Conclusion: The therapy using ADSCs pretreated with HGF and PRP is considered to be a promising therapy.
ID: 1079 Improved glucose homeostasis effect of conditioned media from adipose-derived stem cells in type 1 diabetic mice
Authors: Bui Anh Nguyen Tu , Nguyen Cong Le Thanh , Phan Ngoc Kim , Dang Loan Thi-Tung
Published: September 2017

Background: Many studies suggested adipose derived stem cell (ASC) transplantation as a new approach to control hyperglycemia in type 1 diabetes mellitus. It is proposed that the effects of these cells could be not only based on the direct cell-cell interaction but also the secretion of cytokines. This study aimed to demonstrate the effect of adipose stem cell-derived conditioned medium (CM) on the treatment of STZ-induced diabetic mice.

 Methods: CM was obtained from 24-hours-cultured medium of ASCs and centrifuged to remove the debris. Type 1 diabetic mice were intraperitoneally injected CM for 30 consecutive days. Therapeutic efficacy of CM was assessed by survival rate, blood glucose level, serum insulin level, histological structure of pancreatic islets.

Results: The results showed that CM treatment could decrease mortality rate (from 33,33% to 0%) as well as blood glucose level (from 425,667±65,753 mg/dl to 203,500 mg ±20,350 mg/dl) and enhance insulin secretion, improve size and function of pancreatic islets of diabetic mice.

 Conclusion: Conditioned medium maybe a promising therapy for type 1 diabetes mellitus.
ID: 1080 Adipose-derived stem cells alleviate glucose tolerance in type 2 diabetic mouse
Authors: Bui Anh Nguyen Tu , Nguyen Cong Le Thanh , Nguyen Anh Thi Minh , Truong Nhat Chau , Phan Ngoc Kim , Dang Linh Thi Tung
Published: September 2017

Background: Type 2 diabetes (T2D) is the most common form of diabetes and accounts for 90-95% of all existing diabetic cases. The main etiologies of T2D include insulin resistance in target tissues, insufficient secretion of insulin and subsequent decline of pancreatic β-cell function. Recently, many studies have suggested that adipose – derived stem cells (ASCs) were potential to alleviate insulin resistance and hyperglycemia and promote the islets repair. In this study, ASCs were hypothesized that they could have ameliorative effects on type 2 diabetic mice.

 Methods: Type 2 diabetic mice were induced by a combination of high-fat diet and injection of STZ 100 mg/kg and NA 120 mg/kg. Thereafter, two doses of 106 human ASCs were transplanted 2 week interval into each mouse via the tail vein. The mice were monitored health condition, rate of mortaity, body weight, consumption of food and water, blood glucose level, serum insulin level and histological structure of pancreatic islets.

 Results: Our results indicated that the ASC-treated mice expressed improved condition in comparision with non-treated diabetic mice. The consumption of food and water as well as the blood glucose level decreased. Simultaneously, ASC transplantation improved the impaired glucose tolerance and insulin tolerance in T2D mice. Besides, the total cholesterol have significantly decreased.

 Conclusion: it is suggested that human ASCs infusion is safe and effective for type 2 diabetes mellitus in mice regarding the improved glucose metabolism and insulin resistance.
ID: 1088 Experimental culturing of chick embryo in shell-less culture system – the first research in Vietnam
Authors: Nguyen Quy Xuan , Dang Long Thanh
Published: September 2017

Chick embryo is an ideal model with numerous applications in biomedical research. Among a variety of methods have been carried out for culturing chick embryos, shell-less culture system has a large number of advantages on accessibility, observation and manipulation. In this study, chick embryos were transferred to the shell-less culture system and the development of the chick embryos were assessed. Correlation between the diameter of sinus terminalis on the surface of yolk sac and viability of the embryos would be evaluated. In addition, calcium lactate was added to the culture system in order to find out the optimal amount. After the experiments, results showed that there was no difference between embryos in shell-less culture system and traditional method during incubation period. Secondly, the proportion of live chick embryos until embryonic day 17 reached the highest rate at 87,5% when the diameter of sinus terminalis was between 16 and 21 mm. At last, there was no significant difference between the group with 250 mg calcium lactate supplemented as compared to no supplemented group. Calcium lactate had a lethal effect on chick embryos when the supplemented content was 550 mg. In conclusion, the shell-less culture system could be able to allow the survival of chick embryos until day 21, with high rate in day 17. Besides, this has been the very first time the shell-less culture system was performed in Vietnam.
James Burnes (1801–1862)
Authors: Deschamps Simon
Published: September 2017

James Burnes (1801–1862) was one of the most charismatic and polarizing figures of nineteenth century Scottish freemasonry. He is best remembered for his work on the history of the Knight Templars and as the primary mover of the first Indian lodge specifically designed to welcome native candidates. In the Indian presidency of Bombay, he became an enthusiastic promoter of freemasonry and a zealous political agent defending British colonial interests wherever his travels took him. Although much has been written about his masonic career in India, there is no extensive biography of James Burnes, or at least no satisfactory attempt at a biographical approach that would seek to situate his masonic career within the more general frame of his career as a soldier and empire-builder. This study is therefore meant both as biographical approach that seeks to attempt to offer a more accurate insight into the life and works of this fascinating character, and as an insight into the intricate relationship between freemasonry and imperialism.
Crossing Gender and Colour Lines in American Fraternalism
Authors: Tyssens Jeffrey
Published: September 2017

At first sight, Joseph W. Kinsley could be considered as one of those uncountable ‘grandees’ of American fraternalism whose lives have all fallen into a justified oblivion. Closer scrutiny however reveals a career within different fraternal societies that went far beyond the habitual accumulation of titles and responsibilities in the mainstream orders. Kinsley endeavoured experiments with new types of fraternities wherein the normal exclusion of women and African Americans was to be transcended, first in a frontier town in Montana, later in the federal capital. If all of these orders eventually failed, their short-lived histories nevertheless shed light on those men and women, black and white, who tried to make use of the specific context of the American west to break with segregation and gender exclusiveness. They brought this new formula to other parts of the country. Whatever the limitations of his projects and of the views that he held on race and gender, Kinsley does appear as one of the lonely pioneers who tried to break with deep-rooted fraternal orthodoxies regarding these same categories.
Population dynamics of the brine shrimp Artemia persimilis Piccinelli & Prosdocimi, 1968 (Crustacea, Anostraca) in a hypersaline lake of the Central Pampa (Argentina)
Authors: Vignatti Alicia M. , Cabrera Gabriela C. , Echaniz Santiago A.
Published: September 2017

Abstract The anostracan Artemia is one of the few organisms that can inhabit hypersaline lakes. In Argentina, this genus is represented by two species: the invasive A. franciscana Kellogg, 1906 and the autochthonous A. persimilis Piccinelli & Prosdocimi, 1968, the latter being the only one recorded in the province of La Pampa. Some of the biological aspects of A. persimilis are known based on laboratory studies, and data on its ecology in natural conditions are scarce. The aim of this work is to present information on the density, biomass, and population structure of A. persimilis in relation to environmental variables in Utracán, a hypersaline lake of the semiarid Central Pampa of Argentina. Water and zooplankton samples were taken monthly from May 2009 until August 2010. The mean density and biomass were 56.98 ind.L-1 (± 106.64) and 1.23 mg.L-1 (± 1.35), respectively. The species was negatively affected by salinity, and it was absent between November and February, when salinity exceeded 320 g.L-1. However, when it was present, even during the months of higher salinity, a high proportion of naupliar stages was found, which could indicate that, in nature, A. persimilis can reproduce with high salinity. Although it has been indicated that A. persimilis could be displaced by A. franciscana, the highest tolerance of the former at low temperatures, which allowed the population to continue reproducing at values close to 3°C, would limit its displacement. However, an increase in the temperature of lakes as a result of global warming could modify this situation and allow the advance of the introduced species to the south.
Operadores-escada generalizados para sistemas quânticos
Authors: Batael Hugo de Oliveira , Silva Josimar Fernando da , Silva Alexandre Nogueira da , Santos Saiara Fabiana Menezes dos , Drigo Filho Elso
Published: September 2017

A superálgebra vem sendo aplicada na resolução de diversos problemas quânticos. Ela permite, por exemplo, obter soluções para potenciais exatamente solúveis e ampliar a construção de operadores de criação e destruição. Neste trabalho, o método de construção dos operadores-escada generalizados é revisado e usado para obter os autovalores de energia e as autofunções de dois potenciais, a partícula em uma caixa unidimensional e o potencial de Rosen-Morse unidimensional.
Base stability of deep excavation in clay reinforced with grout piles
Authors: Su Shi-Fon , Liao Hung-Jiun
Published: September 2017

For clay reinforced with grout piles, its shear strength is not easy to determine because the overall strength characteristic of the reinforced soil is the combined result of the shear strengths of the clay and grout piles,  improvement ratio, stress path, boundary condition and so on. The purpose of this study is to develop an undrained stability analysis for the base stability of an excavation when grout piles are installed. This analysis is derived by combining the circular slip mechanism and two equivalent shear strengths: (a) the axial compression undrained shear strength of clay for the slip surface outside the excavation; (b) the axial extension undrained shear strength of clay reinforced with grout piles for the slip surface inside the excavation. This study indicates that the difference between the factor of safety calculated from the proposed method and that predicted from the anisotropic bearing capacity method is limited. However, the applicability of the proposed method to excavations with a longer depth of retaining wall (D > 50·0 m) and a narrower width of excavation (B < 0·80D) is still subject to further investigation.