bluuebunny/crossref_metadata_2025 huggingface dataset:
Validation of an ICH Q2 Compliant Flow Cytometry-Based Assay for the Assessment of the Inhibitory Potential of Mesenchymal Stromal Cells on T Cell Proliferation
Authors: Piede Natascha , Bremm Melanie , Farken Anne , Pfeffermann Lisa-Marie , Cappel Claudia , Bonig Halvard , Fingerhut Theres , Puth Laura , Vogelsang Kathrin , Peinelt Andreas , Marschalek Rolf , Müller Matthias , Bader Peter , Kuçi Zyrafete , Kuçi Selim , Huenecke Sabine
Published: March 2023

Mesenchymal stromal cells (MSCs) have the potential to suppress pathological activation of immune cells and have therefore been considered for the treatment of Graft-versus-Host-Disease. The clinical application of MSCs requires a process validation to ensure consistent quality. A flow cytometry-based mixed lymphocyte reaction (MLR) was developed to analyse the inhibitory effect of MSCs on T cell proliferation. Monoclonal antibodies were used to stimulate T cell expansion and determine the effect of MSCs after four days of co-culture based on proliferation tracking with the violet proliferation dye VPD450. Following the guidelines of the International Council for Harmonisation (ICH) Q2 (R1), the performance of n = 30 peripheral blood mononuclear cell (PBMC) donor pairs was assessed. The specific inhibition of T cells by viable MSCs was determined and precision values of <10% variation for repeatability and <15% for intermediate precision were found. Compared to a non-compendial reference method, a linear correlation of r = 0.9021 was shown. Serial dilution experiments demonstrated a linear range for PBMC:MSC ratios from 1:1 to 1:0.01. The assay was unaffected by PBMC inter-donor variability. In conclusion, the presented MLR can be used as part of quality control tests for the validation of MSCs as a clinical product.
HDL-Associated Proteins in Subjects with Polycystic Ovary Syndrome: A Proteomic Study
Authors: Butler Alexandra E. , Moin Abu Saleh Md , Reiner Željko , Sathyapalan Thozhukat , Jamialahmadi Tannaz , Sahebkar Amirhossein , Atkin Stephen L.
Published: March 2023

Introduction. Serum lipoproteins, with the exception of high-density lipoprotein cholesterol (HDL-C), are increased in polycystic ovary syndrome (PCOS) and their levels may reflect the associated obesity and insulin resistance, but the nature of this association is not fully explained. Therefore, proteomic analysis of key proteins in lipoprotein metabolism was performed. Methods. In this cohort study, plasma was collected from 234 women (137 with PCOS and 97 controls without PCOS). Somalogic proteomic analysis was undertaken for the following 19 proteins involved in lipoprotein, and particularly HDL, metabolism: alpha-1-antichymotrypsin; alpha-1-antitrypsin; apolipoproteins A-1, B, D, E, E2, E3, E4, L1, and M; clusterin; complement C3; hemopexin; heparin cofactor II; kininogen-1; serum amyloid A-1; amyloid beta A-4; and paraoxonase-1. Results. The levels of apolipoprotein E were higher in PCOS (p = 0.012). However, the other isoforms of ApoE, ApoE2, E3, and E4, did not differ when compared with controls. ApoM was lower in PCOS (p = 0.000002). Complement C3 was higher in PCOS (p = 0.037), as was heparin cofactor II (HCFII) (p = 0.0004). The levels of the other proteins associated with lipoprotein metabolism did not differ between PCOS and controls. Conclusions. These data contribute to the concern of the deleterious dyslipidemia found in PCOS, with the novel combination reported here of higher levels of ApoE, C3 and HCFII together with lower ApoM. The dysregulation of these proteins could circumvent the protective effect of HDL-C and contribute to a more atherogenic profile that may increase cardiovascular risk.
Abietane Diterpenoids Isolated from Torreya nucifera Disrupt Replication of Influenza Virus by Blocking the Phosphatidylinositol-3-Kinase (PI3K)-Akt and ERK Signaling Pathway
Authors: Bae Jaehoon , Kwon Hyung-Jun , Park Ji Sun , Jung Jinseok , Ryu Young Bae , Kim Woo Sik , Lee Ju Huck , Jeong Jae-Ho , Lim Jae Sung , Lee Woo Song , Park Su-Jin
Published: March 2023

Although vaccines and antiviral drugs are available, influenza viruses continue to pose a significant threat to vulnerable populations globally. With the emergence of drug-resistant strains, there is a growing need for novel antiviral therapeutic approaches. We found that 18-hydroxyferruginol (1) and 18-oxoferruginol (2) isolated from Torreya nucifera exhibited strong anti-influenza activity, with 50% inhibitory concentration values of 13.6 and 18.3 μM against H1N1, 12.8 and 10.8 μM against H9N2, and 29.2 μM (only compound 2) against H3N2 in the post-treatment assay, respectively. During the viral replication stages, the two compounds demonstrated stronger inhibition of viral RNA and protein in the late stages (12–18 h) than in the early stages (3–6 h). Moreover, both compounds inhibited PI3K-Akt signaling, which participates in viral replication during the later stages of infection. The ERK signaling pathway is also related to viral replication and was substantially inhibited by the two compounds. In particular, the inhibition of PI3K-Akt signaling by these compounds inhibited viral replication by sabotaging influenza ribonucleoprotein nucleus-to-cytoplasm export. These data indicate that compounds 1 and 2 could potentially reduce viral RNA and viral protein levels by inhibiting the PI3K-Akt signaling pathway. Our results suggest that abietane diterpenoids isolated from T. nucifera may be potent antiviral candidates for new influenza therapies.
The Insulin Receptor Substrate 2 Mediates the Action of Insulin on HeLa Cell Migration via the PI3K/Akt Signaling Pathway
Authors: Martínez Báez Anabel , Castro Romero Ivone , Chihu Amparan Lilia , Castañeda Jose Ramos , Ayala Guadalupe
Published: March 2023

Insulin signaling plays an important role in the development and progression of cancer since it is involved in proliferation and migration processes. It has been shown that the A isoform of the insulin receptor (IR-A) is often overexpressed, and its stimulation induces changes in the expression of the insulin receptor substrates (IRS-1 and IRS-2), which are expressed differently in the different types of cancer. We study the participation of the insulin substrates IRS-1 and IRS-2 in the insulin signaling pathway in response to insulin and their involvement in the proliferation and migration of the cervical cancer cell line. Our results showed that under basal conditions, the IR-A isoform was predominantly expressed. Stimulation of HeLa cells with 50 nM insulin led to the phosphorylation of IR-A, showing a statistically significant increase at 30 min (p ≤ 0.05). Stimulation of HeLa cells with insulin induces PI3K and AKT phosphorylation through the activation of IRS2, but not IRS1. While PI3K reached the highest level at 30 min after treatment (p ≤ 0.05), AKT had the highest levels from 15 min (p ≤ 0.05) and remained constant for 6 h. ERK1 and ERK2 expression was also observed, but only ERK2 was phosphorylated in a time-dependent manner, reaching a maximum peak 5 min after insulin stimulation. Although no effect on cell proliferation was observed, insulin stimulation of HeLa cells markedly promoted cell migration.
A Conceptual Model to Strengthen Integrated Management of HIV and NCDs among NIMART-Trained Nurses in Limpopo Province, South Africa
Authors: Murudi-Manganye Nthuseni Sharon , Makhado Lufuno , Sehularo Leepile Alfred
Published: March 2023

Integrated management of human immune deficiency virus (HIV) and non-communicable diseases (NCDs) in primary health care facilities remains a challenge. Despite research that has been conducted in South Africa, it is evident that in Limpopo Province there are slits in the implementation thereof. There is a need to develop a conceptual model to guide in strengthening the clinical competence of nurse-initiated management of antiretroviral therapy (NIMART)-trained nurses to implement the integrated management of HIV and NCDs to improve clinical outcomes of patients with the dual burden of diseases in Limpopo Province, South Africa. This study aimed to develop a conceptual model to strengthen the implementation of integrated management of HIV and NCDs amongst NIMART nurses to improve clinical outcomes of patients with the dual burden of communicable and non-communicable diseases in Limpopo Province, South Africa. An explanatory, sequential, mixed-methods research design was followed. Data were collected from patient records and the skills audit of 25 Primary Health Care (PHC) facilities and from 28 NIMART trained nurses. Donabedian’s structure process outcome model and Miller’s pyramid of clinical competence provided a foundation in the development of the conceptual model. The study revealed a need to develop a conceptual model to strengthen the implementation of integrated HIV and NCDs implementation in PHC, as evidenced by differences in the management of HIV and NCDs. Conclusion: The study findings were conceptualised to describe and develop a model needed to strengthen the implementation of integrated management of HIV and NCDs amongst NIMART nurses working in PHC facilities. The study was limited to Limpopo Province; the model must be implemented in conjunction with the available frameworks to achieve better clinical outcomes.
Nondestructive Evaluation of Fiber-Reinforced Polymer Using Microwave Techniques: A Review
Authors: Tonga Danladi Agadi , Akbar Muhammad Firdaus , Shrifan Nawaf H. M. M. , Jawad Ghassan Nihad , Ghazali Nor Azlin , Packeer Mohamed Mohamed Fauzi , Al-Gburi Ahmed Jamal Abdullah , Ab Wahab Mohd Nadhir
Published: March 2023

Carbon-fiber-reinforced polymer (CFRP) is widely acknowledged as a leading advanced material structure, offering superior properties compared to traditional materials, and has found diverse applications in several industrial sectors, such as that of automobiles, aircrafts, and power plants. However, the production of CFRP composites is prone to fabrication problems, leading to structural defects arising from cycling and aging processes. Identifying these defects at an early stage is crucial to prevent service issues that could result in catastrophic failures. Hence, routine inspection and maintenance are crucial to prevent system collapse. To achieve this objective, conventional nondestructive testing (NDT) methods are utilized to inspect CFRP components. However, the restricted field penetration within the CFRP makes conventional NDT approaches ineffective. Recently, microwave techniques have been developed to address the challenges associated with CFRP inspection by providing better material penetration and more precise results. This paper offers a review of the primary NDT methods employed to inspect CFRP composites, emphasizing microwave-based NDT techniques and their key features.
Pressure Sensing of Symmetric Defect Photonic Crystals Composed of Superconductor and Semiconductor in Low-Temperature Environment
Authors: Hu Haiyang , Chen Xiaoling , Zhao Miaomiao , Wang Liyan , Fang Ming , Zhao Dong
Published: March 2023

We theoretically investigate the defect mode transmittance of light waves in superconductor–semiconductor photonic crystals and its pressure-sensing dependence. The photonic crystal is composed of alternating superconducting and semiconducting slabs and a defect locates at the center of this structure. Two trapezoid waveguides are fixed at both sides of the crystal, which induces the hydrostatic pressure applied and beams transmitted simultaneously. The resonant wavelength variation in the defect mode is directly proportional to the pressure applied on the system in the near-IR region, which can be utilized for linear pressure sensors in the cryogenic environment. Pressure sensitivity reaches a high value of 2.6 nm/GPa, which is higher than that in the study based on the reflection spectra. The sensitivity coefficient may be modulated by the environment temperature as well. This study has potential regarding pressure-light-wave sensors.
Effect of Laser Conditioning on Surface Modification and Laser Damage Resistance of SiO2 Antireflection Film
Authors: Zhang Lijuan , Jiang Xiaolong , Chen Jing , Zhang Chuanchao , Yan Lianghong , Wang Haijun , Luan Xiaoyu , Liao Wei , Jiang Xiaodong , Jiang Yong
Published: March 2023

SiO2 sol-gel antireflection film coated on fused silica can reduce the reflection loss and improve the transmittance of the optical component, although it is still prone to laser induced damage. Laser conditioning is an effective way to improve the laser induced damage threshold (LIDT) of SiO2 sol-gel antireflection film. In this paper, single-layer SiO2 sol-gel antireflection films pretreated by triple-frequency laser with different parameters are characterized by the macroscopical parameters, such as transmittance, refractive index, and thickness. The law of surface modification and the defect removal mechanism of the SiO2 sol-gel antireflection film by laser conditioning are obtained. It is found that laser conditioning can reduce the thickness of the film and introduce densification. In addition, laser conditioning can eliminate micro-defects, such as vacancies and voids in the preparation of SiO2 sol-gel antireflection films, which is the main reason to improve the laser damage resistance of films. Finally, the laser conditioning process with three step laser energy combinations of (0.2–0.6–1.0) Fth0 (zero damage threshold) is the best one to obtain high transmittance, and excellent effects on structure modification and defect removal of films. The research in this paper provides data support for the engineering application and mechanism research of laser conditioning.
Primary Prophylaxis Lapelga® in Early Breast Cancer: A Real-World Experience
Authors: Khan Fahad , Black Morgan , Charlton Alaina , Younus Jawaid
Published: March 2023

Background: Lapelga® was approved by Health Canada as a pegfilgrastim biosimilar in 2019 and remains the most commonly used biosimilar in Ontario and is fully reimbursed under the Ontario Drug Benefit program in this category. We explored the efficacy and tolerability of Lapelga® in a retrospective analysis of patients with early breast cancer who underwent adjuvant chemotherapy supported with Lapelga® as a primary prophylaxis. Methods: Adult patients with early breast cancer treated with adjuvant chemotherapy at the London Regional Cancer Program in London, ON, Canada between May 2019 and June 2022 were included. All of these patients were supported with Lapelga® as the primary prophylaxis. Patients’ age, tumour, and nodal status, their type of chemotherapy, co-morbid conditions, and incidence of febrile neutropenia (FN) and its related details as well as any reported side effects to Lapelga® were collected. Results: A total of 201 patients were included in this review with majority (78%) of patients under 65 years of age. One third of patients were treated with the adriamycin and cyclophosphamide (AC)-Paclitaxel dose dense chemotherapy and a quarter of patients with either a docetaxel and cyclophosphamide (TC) combination or an AC-dose dense with Paclitaxel weekly, and 10% or less patients had FEC-D (5-fluorouracil, epirubicin, and cyclophosphamide) and AC chemotherapy. FN incidence was only 3.48% in this review (7/201 patients). Patients with FN were admitted to hospital and recovered completely with no mortality reported. No cases of a switch to a different granulocyte colony growth factor were seen. The most frequent side effects from Lapelga® included musculoskeletal pain, fever, and headache. However, the majority of patients (88.6%; 178/201) did not have any reported side effects specifically assigned to Lapelga®. Conclusions: In this single centre retrospective study, early breast cancer patients (n = 201) treated with adjuvant chemotherapy supported with primary prophylaxis with Lapelga® had a low incidence of FN (3.48%). This supports Lapelga® being an effective strategy as the primary prophylaxis when used with common chemotherapy regimens in the real-world setting.
Molecular Diversity of BoNT-Producing Clostridia—A Still-Emerging and Challenging Problem
Authors: Grenda Tomasz , Jarosz Aleksandra , Sapała Magdalena , Stasiak Karol , Grenda Anna , Domaradzki Piotr , Kwiatek Krzysztof
Published: March 2023

The diversity of BoNT-producing Clostridia is still a worrying problem for specialists who explore the evolutionary and taxonomic diversity of C. botulinum. It is also a problem for epidemiologists and laboratory staff conducting investigations into foodborne botulism in humans and animals, because their genetic and phenotypic heterogeneity cause complications in choosing the proper analytical tools and in reliably interpreting results. Botulinum neurotoxins (BoNTs) are produced by several bacterial groups that meet all the criteria of distinct species. Despite this, the historical designation of C. botulinum as the one species that produces botulinum toxins is still exploited. New genetic tools such as whole-genome sequencing (WGS) indicate horizontal gene transfer and the occurrence of botulinum gene clusters that are not limited only to Clostridium spp., but also to Gram-negative aerobic species. The literature data regarding the mentioned heterogeneity of BoNT-producing Clostridia indicate the requirement to reclassify C. botulinum species and other microorganisms able to produce BoNTs or possessing botulinum-like gene clusters. The aim of this study was to present the problem of the diversity of BoNT-producing Clostridia over time and new trends toward obtaining a reliable classification of these microorganisms, based on a complex review of the literature.
Fantastic Flatworms and Where to Find Them: Insights into Intertidal Polyclad Flatworm Distribution in Southeastern Australian Boulder Beaches
Authors: Tosetto Louise , McNab Justin M. , Hutchings Pat A. , Rodríguez Jorge , Williamson Jane E.
Published: March 2023

There is a rapid and extensive decline of our marine biodiversity due to human impacts. However, our ability to understand the extent of these effects is hindered by our lack of knowledge of the occurrence and ecology of some species groups. One such group of understudied organisms are marine flatworms of the order Polycladida, a conspicuous component of southeastern Australia’s marine ecosystems that has received little attention over the years. Intertidal boulder beaches support a diverse range of polyclad flatworms in other countries, but the role of these environments in maintaining biodiversity is not well understood. In this study, we identified hotspots of flatworm occurrence by assessing the diversity and overall abundance of flatworms at boulder beaches along the southeast Australian coast. Bottle and Glass, Sydney Harbour, was found to be the most diverse site for flatworms. We also identified a higher occurrence of flatworms under large boulders and less exposed beaches and noted an increased presence of flatworms at higher latitudes. Probable influences on these patterns such as the requirement for shelter and protection are discussed. This study contributes to our knowledge of Australia’s coastal biodiversity and can be used to assist in the management and conservation of our marine environments.
Bizarre Morphology Obscures Real Affiliation: An Integrative Study of Enigmatic Cephalaspid Philine denticulata from Arctic Waters Reveals Its Unique Phylogenetic Position
Authors: Chaban Elena , Ekimova Irina , Lubin Pavel , Nikitenko Ekaterina , Schepetov Dimitry
Published: March 2023

The biodiversity of Cephalaspidea (Gastropoda: Heterobranchia) is poorly studied, and novel findings often lead to revisions at different taxonomic levels. The family Philinidae has a distinct set of defining characters in the shell, copulatory apparatus, and gizzard morphology, but several species, considered part of the family, deviate from it significantly. Philine denticulata (J. Adams, 1800) was considered to be a Philinidae despite the species morphology not fitting well with the family diagnosis. This species has an oval cylindrical external shell, jaws, and a gizzard lined with a chitinous layer with three thickened ridges. We studied Philine denticulata morphology on samples from the White and Barents Seas using the light and scanning electron microscopy methods in addition to computer microtomography. We also reconstructed its phylogenetic position using COI, 16S, 28S, and H3 genetic markers. Our integrative analysis revealed close relationships of this species to the family Aglajidae. Thus, we describe a new genus Philinissima gen. nov., including a single species Philinissima denticulata (J. Adams, 1800) comb. nov. which is the first aglajid registered from the Arctic waters. Our findings highlight possible problems in the Aglajidae taxonomical composition and an overall need for a dedicated integrative revision of the Cephalaspidea.
Distribution of the Riparian Salix Communities in and around Romanian Carpathians
Authors: Bita-Nicolae Claudia
Published: March 2023

Salix riparian communities are particularly diverse and of extraordinary ecological importance. This study will analyze the diversity of Salix riparian communities (S. alba, S. fragilis, S. purpurea and S. triandra), their distribution, ecological importance, and conservation. There were 444 records for S. alba, 417 for S. fragilis, 457 for S. purpurea, and 375 for S. triandra, both from the literature and herbaria. Thus, it can be seen that the distribution of the four Salix species studied is very widespread throughout the territory where this study was carried out. According to EIVE (Ecological Indicator Values of Europe) but also to the national list values for niche positions and niche widths, they were noted to be very close for all ecological indicators: M (soil moisture), L (light), and T (temperature), but not for the ecological indicator of soil nitrogen (N) availability or R (soil reaction). Obviously, those riparian Salix communities are important for the functions they indicate, primarily for climate change mitigation, but also for regulating water flow, improving water quality, and providing habitats for wildlife. Conservation and management of these important ecosystems are necessary to maintain their biodiversity, and ecological services and strategies that can be used to protect and manage these communities are outlined.
Genetic Diversity of Wisent Bison bonasus Based on STR Loci Analyzed in a Large Set of Samples
Authors: Olech Wanda , Wojciechowska Marlena , Kloch Marta , Perlińska-Teresiak Magdalena , Nowak-Życzyńska Zuza
Published: March 2023

Wisent Bison bonasus is an example of a species saved from extinction and reintroduced into nature after a few decades of captive breeding. There were only twelve founders of the Lowland–Caucasian line (LC) and even fewer (seven out of twelve) of Lowland (LB) animals. The genetic diversity in studies based on pedigree or markers is very low. In this paper, we present a summary of the long-term genetic monitoring conducted for the worldwide population of European bison. We summarized the long-term genetic monitoring studies conducted on the worldwide population of wisents to date. We genotyped 2227 wisents from two genetic lines (LC and LB) and different populations at ten microsatellite loci. We found low polymorphism, with only 2.7 alleles per locus, and much lower values of observed heterozygosity (0.380 and 0.348 in the LC and LB lines, respectively) than expected heterozygosity. The difference between the lines is only noticeable in allele proportions, so the number of markers is not enough to distinguish the two genetic lines. We also present the genetic distance among four free-roaming populations that are geographically close to each other. We found that the genetic distance of one of them is larger than that of the others, which could be the effect of genetic drift.
First Record of Phlebotomus (Larroussius) perfiliewi (Diptera: Psychodidae), Vector of Leishmania infantum and Phleboviruses, in Spain
Authors: González Mikel A. , Ruiz-Arrondo Ignacio , Gutiérrez-López Rafael , Barceló Carlos , Miranda Miguel Á.
Published: March 2023

Phlebotomine sand flies are vectors of several diseases of importance for public health, including leishmaniosis, bartonellosis, and sand fly fevers. An entomological survey on blood-feeding Diptera was conducted in June–November 2020–2021 to know the diversity of insect vectors in Mallorca (Balearic Islands, Spain). Among the vectors collected, Phlebotomus (Larroussius) perfiliewi Parrot, 1930 was found being the first record of this species in Spain. Phlebotomus perfiliewi s.l. is one of the main vectors of Leishmania infantum in the Mediterranean Basin and Central Asia. The identification of this species was confirmed by both morphological features and DNA barcoding. Phylogenetic analyses showed that the specimens captured were Ph. perfiliewi s.s. (99.85–100% homologues from Italy and Algeria specimens), with a sequence divergence of 0.17%. The cytochrome c oxidase subunit I gene clearly separates the three species that make up the Ph. perfiliewi species complex. In addition, we also provide a brief discussion about their identification remarks, phylogenetic relationships, and vector status.
Dry Climate Filters Gymnosperms but Not Angiosperms through Seed Mass
Authors: Qi Yang , Liu Hongyan , Xu Chongyang , Dai Jingyu , Han Biao
Published: March 2023

In the context of climate change in recent years, the fate of woody plant seed has an important impact on forest regeneration. Seed mass is an important reproductive strategy of plants. There are huge differences between gymnosperms (mainly conifers) and angiosperms (flowering plants) in terms of reproduction and hydraulic strategies; however, little is known about changes in seed mass along climate aridity gradients between taxonomical groups such as gymnosperms and angiosperms, which limit our understanding on the fate of woody plants under warming-induced climate drying. We collected seed mass data from a total of 2575 woody plant individuals, including 145 species of gymnosperms and 1487 species of angiosperms, across different climatic zones in China. We mapped the distribution pattern of gymnosperm and angiosperm seed mass in China, with angiosperms being maximal near the 400 mm iso-precipitation line. Our phylogenetic analysis results show that seed mass exhibited significant phylogenic signals (p < 0.001) and was also strongly influenced by functional traits (growth type, fruit type, and dispersal mode). The results of linear regression and hierarchical partitioning analysis showed a stronger correlation between gymnosperm seed mass and environmental factors, and a higher independent aridity index effect on gymnosperm seed mass than angiosperm seed mass. The different patterns of seed mass along a climate aridity gradient between gymnosperms and angiosperms may point to different future fates for these two taxonomic groups, while the higher sensitivity of gymnosperm seed mass to environmental conditions may reduce their reproductive rate under the background of climate warming and drying.
Large Remaining Forest Habitat Patches Help Preserve Wild Bee Diversity in Cultivated Blueberry Bush
Authors: Vega Sergio , Vázquez-Rivera Héctor , Normandin Étienne , Fournier Valérie , Lessard Jean-Philippe
Published: March 2023

Global declines in wild and managed bee populations represent a major concern for the agricultural industry. Such declines result, in part, from the loss of natural and semi-natural habitats in and around agricultural ecosystems. However, remaining forest patches in heavily modified landscapes represent nesting habitats that may be crucial to preserving wild bees and their services. Because wild bees are the main pollinators of fruit crops, preserving potential nesting habitats might be particularly important for the crops’ yield and profitability. Here, we assessed whether the abundance and richness of visiting wild bees in blueberry crops relates to the amount of surrounding forest cover and if so, whether those relationships varied with spatial scale. Specifically, we sampled wild bee communities in 18 blueberry fields during the blooming period in Montérégie, Quebec, Canada, where sampling consisted of pan trap triplets and direct observation of flower visitors on blueberry bushes. Then, we quantified the proportion of forest in radii of 0.5 km, 1 km, and 2 km around each field. Wild bee abundance was positively related to the proportion of forest habitat surrounding the crop field, but the relationship for wild bee richness was less clear. Moreover, these relationships were strongest at 1 and 2 km radii of measured land cover. Overall, pollinator diversity was highest when at least 30% of the surrounding landscape consisted of forest patches, representing a total area of at least 1 km2. Our results suggest that preserving large habitat patches in agricultural landscapes can help prevent further decline in wild bee diversity while maximizing pollination services to fruit crops.
Analysis of Quality Indicators of the Pre-Analytical Phase on Blood Gas Analyzers, Point-Of-Care Analyzer in the Period of the COVID-19 Pandemic
Authors: Brescia Vincenzo , Varraso Lucia , Antonucci Mariantonietta , Lovero Roberto , Schirinzi Annalisa , Mascolo Elisa , Di Serio Francesca
Published: March 2023

Aim of the study: We evaluated and compared blood gas analysis (EGA) non-conformities (NC) considered operator-dependent performed in Point-Of-Care (POC) analyzer as quality indicators (IQ) of the pre-analytical phase. To this end, four different NC registered in the resuscitation departments of the Hospital Polyclinic Bari from the beginning of the pandemic (March 2020) until February 2022 were evaluated. The results obtained were compared with those recorded in the pre-COVID period (March 2018–February 2020) to check if there were differences in number and type. Material and methods: GEM 4000 series blood gas analyzers (Instrumentation Laboratory, Bedford, MA, United States) are installed with integrated Intelligent Quality Management (iQM®), which automatically identify and log pre-analytical errors. All blood gas analyzers are connected to the company intranet and interfaced with the GEM Web Plus (Werfen Instrumentation Laboratory, Bedford, MA, United States) data management information system, which allows the core laboratory to remotely supervise all decentralized POC stations. The operator-dependent process NC were expressed in terms of absolute and relative proportions (percentiles and percentage changes). For performance evaluation, the Mann–Whitney U test, Chi-squared test and Six-Sigma Metric calculation for performance classification were performed. Results: In the COVID period, 31,364 blood gas tests were performed vs. 16,632 tests in the pre-COVID period. The NC related to the suitability of the EGA sample and manageable by the operators were totals of 652 (3.9%) and 749 (2.4%), respectively, in the pre-COVID and COVID periods. The pre-analytical phase IQs used did not show statistically significant differences in the two periods evaluated. The Sigma evaluation did not show an increase in error rates. Conclusions: Considering the increase in the number of EGAs performed in the two periods, the training procedures performed by the core laboratory staff were effective; the clinical users of the POC complied with the indications and procedures shared with the core laboratory without increasing the operator-dependent NCs. Furthermore, the core laboratory developed monitoring activities capable of guaranteeing the maintenance of the pre-analytical quality.
Cutaneous Melanoma and Glioblastoma Multiforme Association—Case Presentation and Literature Review
Authors: Orzan Olguța Anca , Giurcăneanu Călin , Dima Bogdan , Dima Monica Beatrice , Ion Ana , Bălăceanu Beatrice , Nițipir Cornelia , Tudose Irina , Nicolae Cătălina Andreea , Dorobanțu Alexandra Maria
Published: March 2023

The occurrence of both melanoma and glioma was first suggested by the observation of a familial association between these conditions, which was later confirmed by the description of the melanoma–astrocytoma syndrome, an extremely rare, inherited affliction in which people have an increased risk of developing melanoma and nervous system tumors. Taking into consideration the common embryologic precursor, the neuroectoderm, it was hypothesized that this syndrome is associated with a genetic disorder. While some families with germline CDKN2A mutations are prone to develop just melanomas, others develop both melanomas and astrocytomas or even other nervous-system neoplasms. Herein, we report the case of a 63-year-old male patient with no personal or family history of malignancy who had primary melanoma followed by glioblastoma. Our case report suggests that the occurrence of both melanoma and glioblastoma is most likely not coincidental but instead linked to genetic mutations of common embryologic precursors or signaling pathways.
Potential of Urine Biomarkers CHI3L1, NGAL, TIMP-2, IGFBP7, and Combinations as Complementary Diagnostic Tools for Acute Kidney Injury after Pediatric Cardiac Surgery: A Prospective Cohort Study
Authors: Vandenberghe Wim , De Loor Jorien , Francois Katrien , Vandekerckhove Kristof , Herck Ingrid , Vande Walle Johan , Peperstraete Harlinde , Bové Thierry , De Wolf Daniël , Nuytinck Lieve , De Waele Jan J. , Meyer Evelyne , Hoste Eric A. J.
Published: March 2023

Acute kidney injury (AKI) is common after pediatric cardiac surgery (CS). Several urine biomarkers have been validated to detect AKI earlier. The objective of this study was to evaluate urine CHI3L1, NGAL, TIMP-2, IGFBP7, and NephroCheck® as predictors for AKI ≥ 1 in pediatric CS after 48 h and AKI ≥ 2 after 12 h. Pediatric patients (age < 18 year; body weight ≥ 2 kg) requiring CS were prospectively included. Urine CHI3L1, NGAL, TIMP-2, IGFBP7, and NephroCheck® were measured during surgery and intensive care unit (ICU) stay and corrected for urine dilution. One hundred and one pediatric patients were included. AKI ≥ 1 within 48 h after ICU admission occurred in 62.4% and AKI ≥ 2 within 12 h in 30.7%. All damage biomarkers predicted AKI ≥ 1 within 48 h after ICU admission, when corrected for urine dilution: CHI3L1 (AUC-ROC: 0.642 (95% CI, 0.535–0.741)), NGAL (0.765 (0.664–0.848)), TIMP-2 (0.778 (0.662–0.868)), IGFBP7 (0.796 (0.682–0.883)), NephroCheck® (0.734 (0.614–0.832)). Similarly, AKI ≥ 2 within 12 h was predicted by all damage biomarkers when corrected for urine dilution: uCHI3L1 (AUC-ROC: 0.686 (95% CI, 0.580–0.780)), NGAL (0.714 (0.609–0.804)), TIMP-2 (0.830 (0.722–0.909)), IGFBP7 (0.834 (0.725–0.912)), NephroCheck® (0.774 (0.658–0.865)). After pediatric cardiac surgery, the damage biomarkers urine CHI3L1, NGAL, TIMP-2, IGFBP7, and NephroCheck® reliably predict AKI after correction for urine dilution.
Microbial Sampling Using Interdental Brushes and Paper Points around Teeth and Implants: A Pilot Study for Comparison
Authors: Janson Tobias M. , Gager Yann , Hatz Christian R. , Köhler Anne-Katrin , Gartenmann Stefanie J. , Schmidlin Patrick R.
Published: March 2023

Inflammatory periodontal and peri-implant diseases follow dysbiotic shifts in a susceptible host. A well-established tool for microbial sample collection is the use of paper points. The purpose of this pilot study was to evaluate the use of interdental brushes compared to paper points. Biofilm samples were collected with paper points and later interdental brushes from ten patients. Five patients were represented with a community periodontal index of treatment needs (CPITN) of 0–2 around the teeth and an implant with PPD ≤ 5 mm and no radiological bone loss. The remaining five patients had a CPITN ≥ 3 and one implant with peri-implantitis. Microbial samples were analyzed with quantitative polymerase chain reaction (qPCR) and next-generation sequencing (NGS). The results showed higher amounts of DNA in samples taken by interdental brushes but also higher Ct values. Both methods detected Filifactor alocis, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, and Treponema denticola in the majority of samples, while Aggregatibacter actinomycetemcomitans was rarely found. A microbial dysbiosis index showed comparable or higher values in sites with no periodontitis/peri-implantitis with interdental brushes. The results of this pilot study indicate that interdental brushes might be a valid technique for microbial sampling and particularly advantageous in the early detection of dysbiotic shifts around teeth and implants. Larger studies with more participants are needed to validate the proposed microbial sampling method with interdental brushes.
Cone-Beam Computed-Tomography-Derived Augmented Fluoroscopy-Guided Biopsy for Peripheral Pulmonary Nodules in a Hybrid Operating Room: A Case Series
Authors: Chen Lun-Che , Yang Shun-Mao , Malwade Shwetambara , Chang Hao-Chun , Chang Ling-Kai , Chung Wen-Yuan , Ko Jen-Chung , Yu Chong-Jen
Published: March 2023

Lung cancer is the most lethal cancer type in Taiwan and worldwide. Early detection and treatment advancements have improved survival. However, small peripheral pulmonary nodules (PPN) biopsy is often challenging, relying solely on bronchoscopy with radial endobronchial ultrasound (EBUS). Augmented fluoroscopy overlays the intra-procedural cone-beam computed tomography (CBCT) images with fluoroscopy enabling real-time three-dimensional localization during bronchoscopic transbronchial biopsy. The hybrid operating room (HOR), equipped with various types of C-arm CBCT, is a perfect suite for PPN diagnosis and other interventional pulmonology. This study shares the single institute experience of EBUS transbronchial biopsy of PPN with the aid of augmented fluoroscopic bronchoscopy (AFB) and CBCT in an HOR. We retrospectively enrolled patients who underwent robotic CBCT, augmented fluoroscopy-guided, radial endobronchial ultrasound-confirmed transbronchial biopsy and cryobiopsy in a hybrid operating room. Patient demographic characteristics, computed tomography images, rapid on-site evaluation cytology, and final pathology reports were collected. Forty-one patients underwent transbronchial biopsy and 6 received additional percutaneous transthoracic core-needle biopsy during the same procedure. The overall diagnostic yield was 88%. The complications included three patients with pneumothorax after receiving subsequent CT-guided percutaneous transthoracic needle biopsy, and two patients with hemothorax who underwent transbronchial cryobiopsy. Overall, the bronchoscopic biopsy of PPN using AFB and CBCT as precise guidance in the hybrid operating room is feasible and can be performed safely with a high diagnostic yield.
Histological Evaluation of Root Canals by Performing a New Cleaning Protocol “RUA” in Endodontic Surgery
Authors: Iandolo Alfredo , Amato Alessandra , Pisano Massimo , Sangiovanni Giuseppe , Abdellatif Dina , Fornara Roberto , Simeone Michele
Published: March 2023

Aim: To enhance cleaning during retro-preparation in endodontic microsurgery. Materials and Methods: Forty mandibular premolars were instrumented, filled with a single cone technique, and then retro-preparation was performed and assigned to experiment A. In group A1, the cavity created by the retro preparation was cleansed with 2 mL of normal sterile saline. In group A2, the retro cavity was cleaned with 2 mL of sterile saline after the retro preparation. All the irrigation solutions mentioned above were delivered using an endodontic needle with a lateral vent and a gauge of 30. Subsequently, in group A2, 17% EDTA gel and 5.25% gel were inserted into the cavity and activated using ultrasonic tips. After the irrigation protocols, the specimens were decalcified for histological evaluation. Results: In the experiment, the amount of hard tissue debris was significantly greater in group A1 compared to group A2 (p < 0.05). Conclusions: The samples in group A2, where the new protocol was performed, showed statistically significant results.
MS-YOLOv7:YOLOv7 Based on Multi-Scale for Object Detection on UAV Aerial Photography
Authors: Zhao LiangLiang , Zhu MinLing
Published: March 2023

A multi-scale UAV aerial image object detection model MS-YOLOv7 based on YOLOv7 was proposed to address the issues of a large number of objects and a high proportion of small objects that commonly exist in the Unmanned Aerial Vehicle (UAV) aerial image. The new network is developed with a multiple detection head and a CBAM convolutional attention module to extract features at different scales. To solve the problem of high-density object detection, a YOLOv7 network architecture combined with the Swin Transformer units is proposed, and a new pyramidal pooling module, SPPFS is incorporated into the network. Finally, we incorporate the SoftNMS and the Mish activation function to improve the network’s ability to identify overlapping and occlusion objects. Various experiments on the open-source dataset VisDrone2019 reveal that our new model brings a significant performance boost compared to other state-of-the-art (SOTA) models. Compared with the YOLOv7 object detection algorithm of the baseline network, the mAP0.5 of MS-YOLOv7 increased by 6.0%, the mAP0.95 increased by 4.9%. Ablation experiments show that the designed modules can improve detection accuracy and visually display the detection effect in different scenarios. This experiment demonstrates the applicability of the MS-YOLOv7 for UAV aerial photograph object detection.
A Novel Chaotic Image Encryption Scheme Armed with Global Dynamic Selection
Authors: Chen Xin , Wang Qianxue , Fan Linfeng , Yu Simin
Published: March 2023

Due to the equivalent keys revealed by a chosen-plaintext attack or a chosen-ciphertext attack, most of the existing chaotic image encryption schemes are demonstrated to be insecure. In order to improve security performance, some scholars have recently proposed the plaintext-related chaotic image encryption scheme. Although the equivalent effect of a one-time pad is achieved, an additional secure channel is required to transmit the hash values or other parameters related to the plaintext before the ciphertext can be decrypted at the receiving end. Its main drawback is that an absolutely secure channel is needed to transmit the information related to the plaintext, which is not feasible in practical applications. To further solve this problem, this paper proposes a chaotic image encryption scheme based on global dynamic selection of a multi-parallel structure. First, a chaotic sequence is employed to dynamically select DNA encoding rules. Secondly, the permutation with a multi-parallel structure is performed on the DNA-encoded matrix, and the DNA decoding rules are dynamically selected according to another chaotic sequence. Finally, the diffusion rules obtained by the ciphertext feedback mechanism are introduced to determine the dynamic diffusion. Compared with the existing local dynamic encryption schemes, the main advantage of this scheme is that it can realize global dynamic selection, so as to ensure that there is no equivalent key, and it can resist the chosen-ciphertext attack or chosen-plaintext attack and does not need an additional secure channel to transmit parameters related to plaintext, which is practical. A theoretical analysis and numerical experiments demonstrate the feasibility of the method.
The Scientific Contribution of the Kaniadakis Entropy to Nuclear Reactor Physics: A Brief Review
Authors: Martinez Aquilino Senra , de Abreu Willian Vieira
Published: March 2023

In nuclear reactors, tracking the loss and production of neutrons is crucial for the safe operation of such devices. In this regard, the microscopic cross section with the Doppler broadening function is a way to represent the thermal agitation movement in a reactor core. This function usually considers the Maxwell–Boltzmann statistics for the velocity distribution. However, this distribution cannot be applied on every occasion, i.e., in conditions outside the thermal equilibrium. In order to overcome this potential limitation, Kaniadakis entropy has been used over the last seven years to generate generalised nuclear data. This short review article summarises what has been conducted so far and what has to be conducted yet.
Enhancement of Film Cooling Effectiveness in a Supersonic Nozzle
Authors: Somasekharan Nithin , Srikrishnan A. R. , Kumar Harihara Sudhan , Ganesh Krishna Prasad , Mohammad Akram , Velamati Ratna Kishore
Published: March 2023

Film cooling as applied to rocket nozzles is analyzed numerically with emphasis on the assessment of the effect of the mixing of coolant with the hot stream. Cooling performance, as characterized by cooling effectiveness, is studied for three different coolants in the three-dimensional, turbulent flow field of a supersonic convergent-divergent nozzle operating with a hot stream temperature of 2500 K over a range of blowing ratios. The coolant stream is injected tangentially into the mainstream using a diffuser-type injector. Parameters influencing the effectiveness, such as coolant injector configuration and mixing layer, are analyzed. Thermal and species mixing between the coolant and the mainstream are investigated with regard to their impact on cooling effectiveness. The results obtained provide insight into the film cooling performance of the gases and the heat transfer characteristics associated with these three gases. An injector taper angle of 30° results in the most effective cooling among the configurations considered (0°, 15°, 30° and 45°). Mixing of the coolant with the hot stream is examined based on the distributions of velocity, temperature and species. The higher values of cooling effectiveness for Helium are attributed to its thermophysical properties and the reduced rate of mixing with the hot stream. The results further indicate that through optimization of the blowing ratio and the coolant injector configuration, the film cooling effectiveness can be substantially improved.
Implementation and Evaluation of 5G MEC-Enabled Smart Factory
Authors: Rekoputra Nadhif Muhammad , Tseng Chia-Wei , Wang Jui-Tang , Liang Shu-Hao , Cheng Ray-Guang , Li Yueh-Feng , Yang Wen-Hao
Published: March 2023

A 5G network can provide more comprehensive bandwidth connectivity for the industry 4.0 environment, which requires faster and tremendous data transmission. This study demonstrates the 5G network performance evaluation with MEC, without MEC, WiFi 6, and Ethernet networks. Usually, a 5G network engages with Multi-access Edge Computing, providing the computing functions dedicated to the users on edge nodes. The MEC network architecture presents significant facilities, a network schematic, and data transmission routers. The field test performs high-definition streaming video and heavy-traffic load testing to evaluate the performance based on different protocols by comparing throughput, latency, jitter, and packet loss rate. MEC network performance, streaming video performance, and load test evaluation results reveal that the 5G network working with MEC achieved better performance than when it was working without MEC. The MEC can improve data transmission efficiency by dedicated configuration but is only accessible with authentication from mobile network operators (MNOs). Therefore, MNOs should offer industrial private network users partial authentication for accessing MEC functionality to improve network feasibility and efficiency. In conclusion, this work illustrates the 5G network implementation and performance measurement for constructing a smart factory.