bluuebunny/crossref_metadata_2025 huggingface dataset:
§193 and §246 of Philosophia botanica do not support that Linnaeus was a “typologist”
Authors: Lidén Magnus
Published: April 2020

§193 and §246 of Linnaeus's Philosophia botanica have recently been invoked to suggest that Linnaeus used, or even introduced, the “Method of Type”, which implies that a genus is centred around a “typical” species. However, there is no support for this conclusion in either of the two paragraphs, rather the opposite. A recapitulation of the last twenty years’ reassessment of Linnaeus's taxonomic philosophy is given.
A double‐blind placebo‐controlled clinical trial testing the effect of hyperbaric oxygen therapy on brain and cognitive outcomes of mildly cognitively impaired elderly with type 2 diabetes: Study design
Authors: BenAri Ori , Efrati Shai , Hadanny Amir , Sano Mary , Bendlin Barbara B. , Lin HungMo , Liu Xiaoyu , Sela Inbar , Almog Ganit , Livny Abigail , Sandler Israel , Ben‐Haim Simona , Sagi Roy , LeRoith Derek , Schnaider Beeri Michal , Ravona‐Springer Ramit
Published: April 2020

Introduction

Type 2 diabetes (T2D) is a risk factor for dementia. Ischemia due to vascular pathology is hypothesized to be an underlying mechanism for this association. Hyperbaric oxygen therapy (HBOT) is a treatment in which oxygen‐enriched air (up to 100%) is administered to patients in a chamber at a pressure above one atmosphere absolute. HBOT is approved for the treatment of T2D ischemic non‐healing wounds. Evidence from animal studies and small clinical trials suggests that HBOT improves hypoxic/ischemic brain injuries, consequently inducing brain angiogensis, leading to cognitive improvement.

Methods

We present the design of the first double‐blind, placebo‐controlled, clinical trial on brain and cognitive outcomes in elderly (n = 154) with T2D and mild cognitive impairment to compare the effects of HBOT versus sham (normal air with 1.1 ATA pressure in the first and last 5 minutes of the session). Eligible candidates are randomized with equal probability to HBOT and sham. Outcomes are assessed before and after treatment, and at 6‐ and 12‐month follow‐up. The primary cognitive outcome is global cognitive change, indexed by a composite sum of z‐scores of four executive functions and four episodic memory tests. The primary neurobiological outcome is cerebral blood flow (CBF; via arterial spin labeling magnetic resonance imaging [ASL‐MRI]) and cerebral glucose utilization via fluorodeoxyglucose positron emission tomography (FDG‐PET). Secondary outcome measures are specific cognitive domains (executive function and episodic memory) and functional measures (Clinical Dementia Rating sum of boxes, activities of daily living). Efficacy analyses will be performed for the intent‐to‐treat sample.

Discussion

Recent studies suggest that HBOT induces neuroplasticity and improves cognition in post‐stroke and traumatic brain injury patients. However, its effect on cognition, cerebral blood flow, and brain glucose utilization in T2D patients at high dementia risk is yet to be determined. If effective, this study may provide strong evidence for the brain and cognitive benefits of HBOT in this population.
Unusual case of severe fever with thrombocytopenia syndrome showing clinical manifestations in a companion dog
Authors: Nam So‐Jeong , Oh Ye‐In , Kim Hyun‐Jung , Cheon Doo‐Sung , Noh Sung‐Jun , Hong Yeon‐Jung
Published: April 2020

Severe fever with thrombocytopenia syndrome (SFTS) virus is an emerging zoonotic virus in East Asia. However, SFTS virus (SFTSV) has not been reported to cause clinical infection in companion dogs to date. We report the case of a 4‐year‐old companion dog that presented with fever, vomiting, leukocytopenia and thrombocytopenia at a veterinary hospital in the Republic of Korea. It was diagnosed with SFTS, which was confirmed using real‐time reverse transcription PCR, sequencing and an indirect immunofluorescence assay, and recovered after supportive care. Further studies are required to investigate SFTSV infection in companion animals, living in close contact with humans, as well as animal‐to‐human transmission.
The potential of hybrid breeding to enhance leaf rust and stripe rust resistance in wheat
Authors: Beukert Ulrike , Liu Guozheng , Thorwarth Patrick , Boeven Philipp H. G. , Longin C. Friedrich H. , Zhao Yusheng , Ganal Martin , Serfling Albrecht , Ordon Frank , Reif Jochen C.
Published: April 2020

Key message

Hybrid wheat breeding is a promising strategy to improve the level of leaf rust and stripe rust resistance in wheat.

Leaf rust and stripe rust belong to the most important fungal diseases in wheat production. Due to a dynamic development of new virulent races, epidemics appear in high frequency and causes significant losses in grain yield and quality. Therefore, research is needed to develop strategies to breed wheat varieties carrying highly efficient resistances. Stacking of dominant resistance genes through hybrid breeding is such an approach. Within this study, we investigated the genetic architecture of leaf rust and stripe rust resistance of 1750 wheat hybrids and their 230 parental lines using a genome-wide association study. We observed on average a lower rust susceptibility for hybrids in comparison to their parental inbred lines and some hybrids outperformed their better parent with up to 56%. Marker-trait associations were identified on chromosome 3D and 4A for leaf rust and on chromosome 2A, 2B, and 6A for stripe rust resistance by using a genome-wide association study with a Bonferroni-corrected threshold of P < 0.10. Detected loci on chromosomes 4A and 2A were located within previously reported genomic regions affecting leaf rust and stripe rust resistance, respectively. The degree of dominance was for most associations favorable in the direction of improved resistance. Thus, resistance can be increased in hybrid wheat breeding by fixing complementary leaf rust and stripe rust resistance genes with desired dominance effects in opposite parental pools.
Impact of River Water and Bottom Sediment Pollution on Accumulation of Metal(loid)s and Arsenic Species in the Coastal Plants Stuckenia pectinata L., Galium aparine L., and Urtica dioica L.: A Chemometric and Environmental Study
Authors: Jabłońska-Czapla Magdalena , Zerzucha Piotr , Grygoyć Katarzyna
Published: April 2020

The role of water and bottom sediment pollution of a river subjected to a strong industrial anthropo-pressure in coastal plants was investigated. The work presented the influence of polluted environment on accumulation of metal(loid)s (including arsenic and its species) in Stuckenia pectinata L., Galium aparine L., and Urtica dioica L. The study provided important information on the contents of organic and inorganic arsenic species in selected plants and their response to heavy metal and arsenic contamination. The As(III), As(V), AB (arsenobetaine), MMA (monomethylarsonic acid), and DMA (dimethylarsinic acid) ions were successfully separated on the Hamilton PRP-X100 column with high-performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS) techniques. The Pollution Load Index and geo-accumulation Index (Igeo) values clearly indicate significant pollution of the examined ecosystem with heavy metals. The chemometric analysis with the concepts of (Dis)similarity Analysis, Cluster Analysis, and Principal Component Analysis helped to visualize the variability of the As species concentrations and to analyse correlations between sampling point locations and analyte contents.
TNFR1 is associated with short-term mortality in patients with diabetes and acute dyspnea seeking care at the emergency department
Authors: Wändell P. , Carlsson A. C. , Larsson A. , Melander O. , Wessman T. , Ärnlöv J. , Ruge T.
Published: April 2020

Background

Circulating levels of TNF alpha receptor 1 (TNFR1) and 2 (TNFR2) are associated with increased long-term mortality and impaired kidney function.

Aim

To study association between circulating levels of TNFR1 and TNFR2 and short-term mortality in patients with diabetes and dyspnea.

Population and methods

Patients aged ≥ 18 years seeking at emergency department (ED) during daytime on weekdays between December 2013 and July 2018, with diabetes and acute dyspnea, identified at the triage process, were included. Participants (n = 291) were triaged according to Medical Emergency Triage and Treatment System-Adult score, and blood samples were collected. Association between TNFR1 and TNFR2, respectively, and 90-day mortality were estimated by Cox regression models adjusted for age, sex, BMI, creatinine and CRP.

Results

Univariate models showed significant associations between TNFR1 and TNFR2, respectively, and CRP, age and creatinine. TNFR1 and TNFR2 tended to be elevated in patients with the highest triage level, compared to patients with lower triage levels (ns). In longitudinal analyses, TNFR1 but not TNFR2 was associated with increased short-term mortality, HR adjusted for age, BMI and creatinine 1.43 (95% CI 1.07–1.91), but not in the model also adjusted for CRP, HR 1.29 (95% CI 0.94–1.77). In secondary analysis for quartile 4 versus quartiles 1–3 of TNFR1, corresponding HRs were 2.46 (95% CI 1.27–5.15) and 2.21 (95% CI 1.07–2.56).

Conclusions

We found a trend for the association between circulating TNFR1 levels and short-term mortality in patients with diabetes and acute dyspnea at the ED, possibly suggesting an inflammatory pathway for the association.
What’s Wrong with Evolutionary Causation?
Authors: Baedke Jan
Published: April 2020

This review essay reflects on recent discussions in evolutionary biology and philosophy of science on the central causes of evolution and the structure of causal explanations in evolutionary theory. In this debate, it has been argued that our view of evolutionary causation should be rethought by including more seriously developmental causes and causes of the individual acting organism. I use Tobias Uller’s and Kevin Laland’s volumeEvolutionary Causationas well as recent reviews of it as a starting point to reflect on the causal role of agency, individuality, and the environment in evolution. In addition, I critically discuss classical philosophical frameworks of theory change (i.e. Popper’s, Kuhn’s and Lakatos’) used in this debate to understand changing views of evolutionary causation.
Cognitive optimism of distinctive initiatives to foster self-directed and self-regulated learning skills: A comparative analysis of conventional and blended-learning in undergraduate studies
Authors: Onah Daniel F. O. , Pang Elaine L. L. , Sinclair Jane E.
Published: April 2020

Independent learning in massive open online courses (MOOCs) requires considerable effort from the learners themselves. Blended-learning has been recognised to foster independent learning among undergraduate students. With the popularity of the blended-learning approach to teach in traditional educational settings, little has been mentioned on how cohesive this approach is in fostering self-directed learning and self-regulation among university students. This study hopes to explore undergraduate learners in their distinctive study patterns. The study was conducted to investigate a comparative study between students from two departments; Science and Social Science. The aim was to explore the students’ self-directed and self-regulated learning skills in conventional classrooms and aspects of blended-learning embedded in a MOOC platform in two academic years for undergraduates at a top UK university. This study encompasses two case studies; firstly, a combine blended-learning seminar and a conventional seminar classes and a study undertaken with a student of English as a second language (ESL). The blended-learning students were participants who registered in a conventional university and took an optional module in computer security. The second group of students participated in a core module of logic and verification. The second case study was with a final year undergraduate student in Education Studies. The students studied and engaged with the course content using their initiative and directing their learning approaches.
Impact of concomitant obstructive sleep apnea on pulmonary involvement and main pulmonary artery diameter in adults with scleroderma
Authors: Yakut Tugce , Balcan Baran , Karakurt Sait , Direskeneli Haner , Yalcinkaya Yasemin , Peker Yüksel
Published: April 2020

Purpose

Pulmonary involvement is common in adults with scleroderma. The effect of concomitant obstructive sleep apnea (OSA) on risk for pulmonary hypertension in scleroderma is unknown. An enlarged main pulmonary artery diameter (mPAD) derived from chest computer tomography (CT) is a useful predictor of pulmonary hypertension. We addressed the effect of OSA on pulmonary involvement and enlarged mPAD in adults with scleroderma.

Methods

All participants underwent pulmonary function testing, carbon monoxide diffusion capacity, chest CT, and overnight sleep recording with home sleep apnea testing. OSA diagnosis was based on an apnea-hypopnea index (AHI) ≥ 15/h. Oxygen desaturation index (ODI) was also recorded. Scleroderma involvement of the lungs was defined as the Warrick score ≥ 7 based on the CT findings. Enlarged mPAD was defined as an mPAD ≥ 29 mm in men and ≥ 27 mm in women.

Results

After exclusions, 62 patients (58 women) were included. OSA was found among 20 (32%), 17/42 (38%) in the limited cutaneous type, and 3/20 (15%) in the diffuse cutaneous type (p= 0.08). Scleroderma involvement of the lungs was observed in 40 participants (65% in OSA vs 64% in no-OSA; n.s.). Enlarged mPAD was measured in 16 participants, 10 of 20 (50%) in the OSA group and 6 of 17 (14%) in the no-OSA group (p= 0.003). OSA was associated with enlarged mPAD (odds ratio 4.7, 95% confidence interval 1.1–20.9;p= 0.042) independent of age, body mass index, and pulmonary involvement. There was a linear relationship between mPAD and AHI (r= 0.37;p= 0.003) as well as ODI (r= 0.41;p< 0.001).

Conclusions

In this cohort, OSA was associated with risk for pulmonary hypertension independent of pulmonary involvement. These findings suggest that assessing the effect of therapy for concomitant OSA in patients with scleroderma is warranted.

Trial registration

NCT 02740569
Reporting Clinical Significance in Hip Arthroscopy: Where Are We Now?
Authors: Polascik Breanna A. , Peck Jeffrey , Cepeda Nicholas , Lyman Stephen , Ling Daphne
Published: April 2020

Background

Although p values are standard for reporting statistical significance of patient-reported outcome measures (PROMs), the shift toward clinically important outcome values, including minimal clinically important difference (MCID) and substantial clinical benefit (SCB), necessitates re-evaluation of the current literature.

Questions/Purposes

We sought to answer two questions regarding studies on primary hip arthroscopy performed for the treatment of femoroacetabular impingement syndrome (FAIS). (1) Do such studies reporting statistical significance on common PROMs meet published MCID/SCB thresholds? (2) What proportion of such studies report both statistical and clinical significance?

Methods

We identified four papers published in two journals defining MCID/SCB values on the modified Harris Hip Score (mHHS), Hip Outcome Score–Activities of Daily Living (HOS-ADL), Hip Outcome Score-Sport (HOS-Sport), international Hip Outcome Tool (iHOT-33), and its short version (iHOT-12) for different groups of FAIS patients undergoing hip arthroscopy. We reviewed these two journals from the dates of publication to the present to identify papers reporting changes in post-operative PROMs. The difference in pre- and post-operative scores on each PROM was calculated and compared to MCID/SCB thresholds.

Results

Twelve studies were included. Ten studies (83%) evaluated mHHS (90% met MCID, 50% met SCB), seven (58%) evaluated HOS-ADL (100% met MCID/SCB) and HOS-Sport (100% met MCID, 57% met SCB), and one (8%) evaluated iHOT-33 (met MCID/SCB) and iHOT-12 (met MCID). Most studies met MCID and SCB at both 1- and 2-year timepoints. Of the studies evaluated, 50% reported clinical relevance.

Conclusions

Nearly all studies evaluated met MCID, while fewer met SCB. Only half discussed these clinical measures. It is proposed that all future studies report both statistical and clinical significance as standard best practice.
The Nature of Native MgO in Mg and Its Alloys
Authors: Wang Shihao , Wang Yun , Ramasse Quentin , Fan Zhongyun
Published: April 2020

Native MgO particles in Mg-alloy melts have been recently exploited as potential substrates for heterogeneous nucleation during solidification, leading to significant grain refinement of various Mg-alloys. However, our current knowledge of the nature of the native MgO particles is still limited. In this work, we study both the physical and chemical nature of the native MgO in commercial purity Mg and Mg-9Al alloy by means of advanced electron microscopy. We found that as oxidation products MgO aggregates exist in the alloy melt in three different forms: dominantly young oxide film, occasionally old oxide film and ingot skin, all consisting of discrete nano-sized MgO particles. Detailed analysis shows that the native MgO particles have an octahedral or cubic morphology, a nano-scale particle size and a log-normal size distribution. The mechanisms underlying the formation of the two types of MgO were investigated, and we found that octahedral MgO is formed by oxidation of Mg melt and cubic MgO by oxidation of Mg vapor. With a large lattice misfit withα-Mg, the native MgO particles are impotent for heterogeneous nucleation, but can be made effective for grain refinement.
Are aesthetic judgements purely aesthetic? Testing the social conformity account
Authors: Inglis Matthew , Aberdein Andrew
Published: April 2020

Many of the methods commonly used to research mathematical practice, such as analyses of historical episodes or individual cases, are particularly well-suited togeneratingcausal hypotheses, but less well-suited totestingcausal hypotheses. In this paper we reflect on the contribution that the so-called hypothetico-deductive method, with a particular focus on experimental studies, can make to our understanding of mathematical practice. By way of illustration, we report an experiment that investigated how mathematicians attribute aesthetic properties to mathematical proofs. We demonstrate that perceptions of the aesthetic properties of mathematical proofs are, in some cases at least, subject to social influence. Specifically, we show that mathematicians’ aesthetic judgements tend to conform to the judgements made by others. Pedagogical implications are discussed.
Spatial and temporal variability of soil loss in gully erosion in upper Blue Nile basin, Ethiopia
Authors: Bogale Alemsha , Aynalem Dessalew , Adem Anwar , Mekuria Wolde , Tilahun Seifu
Published: April 2020

Gully erosion has many negative impacts on both cultivated and grazing lands in Ethiopian highlands. The present study was conducted in Chentale watershed, Ethiopia, to quantify the contribution of gully erosion, and to assess its temporal changes. Within the Chentale watershed, we selected a sub-watershed (104.6 ha) and nested gully catchment, and gauged for stream flow and sediment concentration data in 2015 and 2016. We measured gully dimensions before and after the onset of the rainy season in 2016 to determine soil loss due to gully erosion. The temporal changes of gully expansion were determined by digitizing gully plain area from Google earth images taken in 2005 and 2013. The results support that gullies were expanding at higher rate in recent years. Area covered by gullies in the watershed increased from 1.84 to 3.43 ha between 2005 and 2013, indicating that the proportion of the watershed covered by gullies was nearly doubled in the investigated period. The estimated soil loss from the main watershed and gullies catchment was 6 and 2 t ha−1 year−1 in 2015, and was 7 and 9 t ha−1 year−1 in 2016, respectively. The results support that gullies were the main contributors of soil erosion in the watershed, and that integrated soil and water conservation measures are required to reduce soil erosion.
Effect of the specific surface area of MgO on the treatment of boron and fluorine
Authors: Kameda Tomohito , Yamamoto Yusuke , Kumagai Shogo , Yoshioka Toshiaki
Published: April 2020

To realize improved wastewater treatment processes, the study investigated the processing of boron and fluorine and the processing of real landfill leachate using MgO with different specific surface areas. By optimizing the MgO absorbent, the time required for boron and fluorine processing can be reduced dramatically and uniform wastewater quality standards can be achieved. The rate constants for boron and fluorine removal increased with both temperature and specific surface area. In the processing of actual wastewater, which contained many coexisting ions, MgO was successfully applied to the removal of boron.
Orbits of homogeneous polynomials on Banach spaces
Authors: CARDECCIA RODRIGO , MURO SANTIAGO
Published: April 2020

We study the dynamics induced by homogeneous polynomials on Banach spaces. It is known that no homogeneous polynomial defined on a Banach space can have a dense orbit. We show a simple and natural example of a homogeneous polynomial with an orbit that is at the same time $\unicode[STIX]{x1D6FF}$-dense (the orbit meets every ball of radius $\unicode[STIX]{x1D6FF}$), weakly dense and such that $\unicode[STIX]{x1D6E4}\cdot \text{Orb}_{P}(x)$ is dense for every $\unicode[STIX]{x1D6E4}\subset \mathbb{C}$ that either is unbounded or has 0 as an accumulation point. Moreover, we generalize the construction to arbitrary infinite-dimensional separable Banach spaces. To prove this, we study Julia sets of homogeneous polynomials on Banach spaces.
A generalized type semigroup and dynamical comparison
Authors: MA XIN
Published: April 2020

In this paper, we construct and study a semigroup associated to an action of a countable discrete group on a compact Hausdorff space that can be regarded as a higher dimensional generalization of the type semigroup. We study when this semigroup is almost unperforated. This leads to a new characterization of dynamical comparison and thus answers a question of Kerr and Schafhauser. In addition, this paper suggests a definition of comparison for dynamical systems in which neither the acting group is necessarily amenable nor the action is minimal.
Preventing healthcare-associated infection in Switzerland: Results of a national survey
Authors: Sax Hugo , Schreiber Peter W. , Clack Lauren , Ratz David , Saint Sanjay , Greene M. Todd , Kuster Stefan P.
Published: April 2020

We assessed infection prevention in Swiss hospitals via a national survey focusing on infection prevention practices prior to a large national infection prevention initiative. Of the 59 hospitals that responded (77%), 98% had infection prevention teams and 40% very good or excellent leadership support. However, a minority of hospitals used recommended infection prevention practices and surveillance systems regularly.
Covid-19 and the N95 respirator shortage: Closing the gap
Authors: Nogee Daniel , Tomassoni Anthony J.
Published: April 2020

Due to extreme shortages of personal protective equipment caused by the COVID-19 pandemic, many healthcare workers will be forced to recycle protective masks intended for disposal after a single use. We propose investigating the use of ultraviolet germicidal irradiation to sterilize masks of SARS-CoV-2 for safer reuse.
CLASSIFICATION OF THE SUBLATTICES OF A LATTICE
Authors: ZONG CHUANMING
Published: April 2020

In 1945–1946, C. L. Siegel proved that an $n$-dimensional lattice $\unicode[STIX]{x1D6EC}$ of determinant $\text{det}(\unicode[STIX]{x1D6EC})$ has at most $m^{n^{2}}$ different sublattices of determinant $m\cdot \text{det}(\unicode[STIX]{x1D6EC})$. In 1997, the exact number of the different sublattices of index $m$ was determined by Baake. We present a systematic treatment for counting the sublattices and derive a formula for the number of the sublattice classes under unimodular equivalence.
Restoring the State's Power to Defame: The Legal Life of Character in the Era of Roosevelt and Trump
Authors: SALAZAR JAMES B.
Published: April 2020

While the abstract equality of citizens before the law is imagined as protection from arbitrary, subjective legal judgments of an individual's character, I argue that judgments of character play a pivotal yet unexamined role regulating access to citizenship in American law. Through a comparative analysis of President Trump and President Theodore Roosevelt, I show how their seemingly personal obsession with libel law reveals a deeper interest in consolidating the state's power as sole arbiter of character in order to weaponize the “good moral character” requirement in immigration and naturalization law as an instrument of racial and ethnic exclusion.
Missing link: California rock crabs serve as intermediate hosts for the parasite Helicometrina nimia
Authors: Shanebeck K.M. , Presswell B. , Lagrue C.
Published: April 2020

Parasites can have strong effects on invertebrate host behaviour, fecundity and survival in marine ecosystems. However, parasites are often poorly documented and still rarely integrated into marine ecological modelling; comprehensive surveys of infection in marine invertebrates are sporadic at best. For example, rock crabs are an important part of Californian coastal ecosystems, both as regulators of mussel populations and non-native species, and as prey items for predators like sea otters, but their parasite communities and potential effects on crab population dynamics are seldom studied or understood. Here, we present the first report of infection by the trematode Helicometrina nimia in the economically and ecologically important red rock crab (Cancer productus) and Pacific rock crab (Romaleon antennarium). As intermediate hosts, they are a missing link for infection by H. nimia in Californian fish that was unreported until now. Based on these findings, we advocate for further research into parasite diversity and their potential effects on ecologically and commercially important species.
Density functional theory for collisionless plasmas – equivalence of fluid and kinetic approaches
Authors: Manfredi Giovanni
Published: April 2020

Density functional theory (DFT) is a powerful theoretical tool widely used in such diverse fields as computational condensed-matter physics, atomic physics and quantum chemistry. DFT establishes that a system of

$N$

interacting electrons can be described uniquely by its single-particle density

$n(\boldsymbol{r})$

, instead of the

$N$

-body wave function, yielding an enormous gain in terms of computational speed and memory storage space. Here, we use time-dependent DFT to show that a classical collisionless plasma can always, in principle, be described by a set of fluid equations for the single-particle density and current. The results of DFT guarantee that an exact closure relation, fully reproducing the Vlasov dynamics, necessarily exists, although it may be complicated (non-local in space and time, for instance) and difficult to obtain in practice. This goes against the common wisdom in plasma physics that the Vlasov and fluid descriptions are mutually incompatible, with the latter inevitably missing some ‘purely kinetic’ effects.
Energizing the plasmalemma of marine photosynthetic organisms: the role of primary active transport
Authors: Raven John A. , Beardall John
Published: April 2020

Generation of ion electrochemical potential differences by primary active transport can involve energy inputs from light, from exergonic redox reactions and from exergonic ATP hydrolysis. These electrochemical potential differences are important for homoeostasis, for signalling, and for energizing nutrient influx. The three main ions involved are H+, Na+(efflux) and Cl−(influx). In prokaryotes, fluxes of all three of these ions are energized by ion-pumping rhodopsins, with one archaeal rhodopsin pumping H+intothe cells; among eukaryotes there is also an H+influx rhodopsin inAcetabulariaand (probably) H+efflux in diatoms. Bacteriochlorophyll-based photoreactions export H+from the cytosol in some anoxygenic photosynthetic bacteria, but chlorophyll-based photoreactions in marine cyanobacteria do not lead to export of H+. Exergonic redox reactions export H+and Na+in photosynthetic bacteria, and possibly H+in eukaryotic algae. P-type H+- and/or Na+-ATPases occur in almost all of the photosynthetic marine organisms examined. P-type H+-efflux ATPases occur in charophycean marine algae and flowering plants whereas P-type Na+-ATPases predominate in other marine green algae and non-green algae, possibly with H+-ATPases in some cases. An F-type Cl−-ATPase is known to occur inAcetabularia. Some assignments, on the basis of genomic evidence, of P-type ATPases to H+or Na+as the pumped ion are inconclusive.
Age and growth of Antimora rostrata (Moridae, Gadiformes, Teleostei) from the Kerguelen and Crozet Islands in the southern Indian Ocean
Authors: Korostelev Nikolai B. , Vedishcheva Elena V. , Orlov Alexei M.
Published: April 2020

Age and growth of blue antimora Antimora rostrata were examined for the first time in the waters of Kerguelen and Crozet Islands (southern Indian Ocean, sub-Antarctic). The longline catches were represented by fish ranging from 39 to 72 cm in total length with weights between 400 and 3310 g, aged 16– 41 years. A minimum age of 16 years was observed in a fish 39 cm long, while a maximum age of 41 years was recorded for an individual of 70 cm in length and 3310 g in weight. The age classes with the greatest numbers were represented by fish aged 34 years (9%), 28 years (9%) and 29 years (8%), which together accounted for 26% of the total catch. The blue antimora in the southern Indian Ocean shows similar growth rates to those of individual fish from the Ross, Lazarev and Weddell Seas and southeastern Greenland, which may indicate the population unity of the species within the Antarctic and sub-Antarctic waters or similar habitat conditions in these areas.
Cellular development of the germinal epithelium during the female and male gametogenesis of Chaetodon striatus (Perciformes: Chaetodontidae)
Authors: Bayona Perez Isabelle Leite , Mazzoni Talita Sarah , Quagio-Grassiotto Irani
Published: April 2020

Butterflyfish Chaetodon striatus is highly sought after in the marine ornamental aquarium, although studies about its reproductive biology are scarce. Therefore, to contribute to a better understanding of the reproductive aspects of C. striatus, we describe in detail with the use of high resolution histology the cellular dynamics of the germinal epithelium during the reproductive life history of this species. Based on the activity of the germinal epithelium, this study describes different stages of the gonadal development, similar to the reproductive phases found in other fish, to determine the reproductive period of C. striatus. In characterization of gonadal development, the following germ cells are described for males: spermatogonia, spermatocytes, spermatids and spermatozoa. Oogonia, early, primary, secondary, full-grown and maturing oocytes are described for females. Female germinal epithelium of C. striatus showed substantial changes over the study period, indicating that there was an active spawning period. Male germinal epithelium also presented relevant alterations, indicating reproductive activity in the testicular lobules. Morphological data confirm how informative was the cellular dynamics of the germinal epithelium for understanding gonadal development during adult reproductive life of fish in general. Although Chaetodon are a popular species, previous studies have only produced superficial and rough histological analyses. Therefore, this study demonstrates important information on germinal epithelium of Chaetodon. This knowledge could be a fundamental tool for development of new strategies for breeding of several species in captivity, especially butterflyfishes.
Fontan completion during winter season is not associated with higher mortality or morbidity in the early post-operative period
Authors: Nordmeyer Sarah , Krettek Sabeth , Nordmeyer Johannes , Schafstedde Marie , Rehm Konstantin , Photiadis Joachim , Berger Felix , Ovroutski Stanislav
Published: April 2020

Objectives:

The aim of our study was to compare post-operative outcome after total cavopulmonary connection between patients operated during winter and summer season.

Methods:

We retrospectively studied 211 patients who underwent extracardiac total cavopulmonary connection completion at our institution between 1995 and 2015 (median age 4 (1–42) years). Seventy (33%) patients were operated during winter (November to March) and 141 (67%) patients during summer season (April to October).

Results:

Patients operated during winter and summer season showed no difference in early mortality (7% versus 5%, p = 0.52) and severe morbidity like need for early Fontan takedown (1% versus 1%, p = 0.99) and need for mechanical circulatory support (9% versus 4%, p = 0.12). The post-operative course and haemodynamic outcome were comparable between both groups of patients (ICU (4 versus 3 days, p = 0.44) and hospital stay (15 versus 14 days, p = 0.28), prolonged pleural effusions (36% versus 31%, p = 0.51), need for dialysis (16% versus 11%, p = 0.37), ascites (37% versus 33%, p = 0.52), supraventricular tachyarrhythmia (16% versus 13%, p = 0.56) and chylothorax (26% versus 16%, p = 0.12), change of antibiotic treatment (47% versus 36%, p = 0.06), prolonged inotropic support (24% versus 14%, p = 0.05), intubation time (15 versus 12 hours, p = 0.33), and incidence of fast-track extubation (11% versus 22%, p = 0.06).

Conclusion:

Outcomes after total cavopulmonary connection completion during winter and summer season were comparably related to mortality, severe morbidity, or longer hospital stay in the early post-operative period. These results suggest that total cavopulmonary connection completion during winter season is as safe as during summer season.
A transition clinic intervention to improve follow-up rates in adolescents and young adults with congenital heart disease
Authors: Gaydos Stephanie S. , Chowdhury Shahryar M. , Judd Rochelle N. , McHugh Kimberly E.
Published: April 2020

Background:

Children with congenital heart disease (CHD) require lifelong cardiology follow-up. Many experience gaps in care around the age of transition to adult-oriented care with associated comorbidity. We describe the impact of a clinic-based intervention on follow-up rates in this high-risk population.

Methods:

Patients ≥11 years seen in a paediatric outpatient CHD Transition Clinic completed self-assessment questionnaires, underwent focused teaching, and were followed on a clinic registry with assessment of care continuation. The cohort “lost to follow-up” rate, defined as absence from care at least 6 months beyond the recommended timeframe, was compared with a control group. Secondary outcomes included questionnaire scores and adult cardiology referral trends.

Results:

Over 26 months, 53 participants completed an initial Transition Clinic visit; 43% (23/53) underwent a second visit. Median participant age was 18.0 years (interquartile range 16.0, 22.0). The cohort’s “lost to follow-up” rate was 7.3%, which was significantly lower than the control rate (25.9%, p < 0.01). Multivariable regression analyses demonstrated clinic participation as the only factor independently associated with follow-up rates (p = 0.048). Transition readiness was associated with older age (p = 0.01) but not sex, univentricular heart, interventional history, or surgical complexity. One-third of adult participants transferred to adult care.

Conclusions:

A CHD Transition Clinic intervention can improve follow-up rates in adolescents and young adults. Age is an important factor in transition readiness, and retention of adults in paediatric care appears multi-factorial. We postulate that serial assessments of self-management, focused education, and registry utilisation may improve patient outcomes by reducing lapses in care.
Political Intrusions into the International Health Regulations Treaty and Its Impact on Management of Rapidly Emerging Zoonotic Pandemics: What History Tells Us
Authors: Burkle Frederick M.
Published: April 2020

For a large number of health care providers world-wide, the coronavirus disease 2019 (COVID-19) pandemic is their first experience in population-based care. In past decades, lower population densities, infectious disease outbreaks, epidemics, and pandemics were rare and driven almost exclusively by natural disasters, predatory animals, and war. In the early 1900s, Sir William Osler first advanced the knowledge of zoonotic diseases that are spread from reservoir animals to human animals. Once rare, they now make up 71% or more of new diseases. Globally, zoonotic spread occurs for many reasons. Because the human population has grown in numbers and density, the spread of these diseases accelerated though rapid unsustainable urbanization, biodiversity loss, and climate change. Furthermore, they are exacerbated by an increasing number of vulnerable populations suffering from chronic deficiencies in food, water, and energy. The World Health Organization (WHO) and its International Health Regulation (IHR) Treaty, organized to manage population-based diseases such as Influenza, severe acute respiratory syndrome (SARS), H1N1, Middle East respiratory syndrome (MERS), HIV, and Ebola, have failed to meet population-based expectations. In part, this is due to influence from powerful political donors, which has become most evident in the current COVID-19 pandemic. The global community can no longer tolerate an ineffectual and passive international response system, nor tolerate the self-serving political interference that authoritarian regimes and others have exercised over the WHO. In a highly integrated globalized world, both the WHO with its IHR Treaty have the potential to become one of the most effective mechanisms for crisis response and risk reduction world-wide. Practitioners and health decision-makers must break their silence and advocate for a stronger treaty, a return of the WHO’s singular global authority, and support highly coordinated population-based management. As Osler recognized, his concept of “one medicine, one health” defines what global public health is today.
The long-term effectiveness and acceptability of the retailer-led removal of unhealthy drinks from display in a self-service café
Authors: Ryan Angela , Huse Oliver , Blake Miranda R , Boelsen-Robinson Tara , Noble Kia , Peeters Anna
Published: April 2020

Objective:

In 2015, beverages were removed from display at a self-service café within a major health service, resulting in fewer purchases of unhealthy beverages. This initiative was continued following initial evaluation of the results. The current study aimed to determine customer acceptability of the initiative, and whether healthier purchases had continued, at 18 months following implementation.

Design:

Drinks were categorised as ‘green’ (best choices), ‘amber’ (choose carefully) and ‘red’ (limit), based on the state government nutrient profiling system, for intervention and analysis purposes. In 2015, unhealthy ‘red’ drinks were removed from display. In 2017, weekly beverage sales were counted, through stock-taking, for 6 weeks, and customer surveys were conducted over 2 days.

Setting:

A café located within a major Victorian health service.

Participants:

Café customers (hospital staff, patients and visitors).

Results:

Eighteen months after the implementation of the initiative, the proportion of ‘red’ beverages sold was 7 % of total drink sales (compared with 33 % before the removal of unhealthy beverages from display in 2015 (P < 0·001), and 10 % immediately following the removal of unhealthy beverages from display). Customer surveys revealed high levels of acceptability for the initiative and low levels of awareness of the initiative.

Conclusions:

The removal of unhealthy beverages from display can result in customers making healthier purchases, and this appears to continue over the long-term. Such interventions have the potential to contribute to the sustained shift in population purchases and consumption needed to make meaningful improvements to population health.
Subsidy Determination, Benchmarks, and Adverse Inferences: Assessing ‘Benefit' in US–Coated Paper (Indonesia)
Authors: Beaulieu Eugene , Prévost Denise
Published: April 2020

This paper presents a legal-economic analysis of key aspects of the WTO Panel Report involving a challenge by Indonesia against the anti-dumping and countervailing duties imposed by the US on certain coated paper from Indonesia. We focus on the findings in this case relevant to the determination of a ‘benefit’ to the recipient, a core requirement to establish the existence and extent of a subsidy. We examine benchmarking for determining benefit in cases of predominant government ownership of a natural resource and the use of ‘adverse facts available’ against a non-cooperative respondent to infer the existence of a benefit. The benefit analysis in this case may have broader implications. First, it may limit the scope for governments to determine their own policies regarding the ownership and management of natural resources. Second, it may create a loophole allowing investigating authorities to fill gaps in the factual record by intentionally using the ‘facts available’ to the disadvantage of a respondent. In both cases, the panel's findings may open the door to potential misuse of these flexibilities to find a benefit where none exists, or to inflate the margin of benefit to allow for higher countervailing duties.
The practice and research of cognitive behavioural therapy in Egypt: a review of the current status
Authors: Aly Reham , Ramy Hisham , Rathod Shanaya
Published: April 2020

Cognitive behavioural therapy (CBT) is generally defined as a short-term, problem-oriented psychotherapy that focuses on modifying dysfunctional emotions, thoughts and actions. In clinical practice, CBT consists of a number of cognitive and behavioural techniques that aim to help patients suffering from psychological disorders. The practice of CBT is well established in Western countries. In non-Western countries, CBT is still in the process of being established as the evidence-based psychotherapy. Despite being the heart of the Arab world, the development and practice of psychotherapy in Egypt in general, and CBT specifically, is still emerging. The training and practice of CBT has received a mixed response in Egypt. In practical settings, evidence-based CBT is subject to individual and cultural variations and adaptations. Many local studies examining the efficacy of CBT in psychiatric disorders among different population groups in Egypt have been conducted. Unfortunately, many of these studies have not been published and therefore have missed the opportunity for international recognition. The current review aims to explore the practice of CBT with a specific focus on national research of efficacy and adaptability of CBT for different populations in Egypt.

Key learning aims

(1)

Current status of the practice and education of CBT in Egypt.

(2)

Body of research conducted on CBT in Egypt.

(3)

Needs and recommendations for further development of CBT provision in Egypt.
Hydrogen Variability in the Murray Formation, Gale Crater, Mars
Authors: Thomas N. H. , Ehlmann B. L. , Rapin W. , Rivera‐Hernández F. , Stein N. T. , Frydenvang J. , Gabriel T. , Meslin P.‐Y. , Maurice S. , Wiens R. C.
Published: April 2020

The Mars Science Laboratory (MSL) Curiosity rover is exploring the Murray formation, a sequence of heterolithic mudstones and sandstones recording fluvial deltaic and lake deposits that comprise over 350 m of sedimentary strata within Gale crater. We examine >4,500 Murray formation bedrock points, employing recent laboratory calibrations for ChemCam laser‐induced breakdown spectroscopy H measurements at millimeter scale. Bedrock in the Murray formation has an interquartile range of 2.3–3.1 wt.% H2O, similar to measurements using the Dynamic Albedo of Neutrons and Sample Analysis at Mars instruments. However, specific stratigraphic intervals include high H targets (6–18 wt.% H2O) correlated with Si, Mg, Ca, Mn, or Fe, indicating units with opal, hydrated Mg sulfates, hydrated Ca sulfates, Mn‐enriched units, and akageneite or other iron oxyhydroxides, respectively. One stratigraphic interval with higher hydrogen is the Sutton Island unit and Blunts Point unit contact, where higher hydrogen is associated with Fe‐rich, Ca‐rich, and Mg‐rich points. A second interval with higher hydrogen occurs in the Vera Rubin ridge portion of the Murray formation, where higher hydrogen is associated with Fe‐rich, Ca‐rich, and Si‐rich points. We also observe trends in the H signal with grain size, separate from chemical variation, whereby coarser‐grained rocks have higher hydrogen. Variability in the hydrogen content of rocks points to a history of water‐rock interaction at Gale crater that included changes in lake water chemistry during Murray formation deposition and multiple subsequent groundwater episodes.
Spatial heterogeneity of oxygenation and haemodynamics in breast cancer resolved in vivo by conical multispectral optoacoustic mesoscopy
Authors: Li Jiao , Chekkoury Andrei , Prakash Jaya , Glasl Sarah , Vetschera Paul , Koberstein-Schwarz Benno , Olefir Ivan , Gujrati Vipul , Omar Murad , Ntziachristos Vasilis
Published: April 2020

The characteristics of tumour development and metastasis relate not only to genomic heterogeneity but also to spatial heterogeneity, associated with variations in the intratumoural arrangement of cell populations, vascular morphology and oxygen and nutrient supply. While optical (photonic) microscopy is commonly employed to visualize the tumour microenvironment, it assesses only a few hundred cubic microns of tissue. Therefore, it is not suitable for investigating biological processes at the level of the entire tumour, which can be at least four orders of magnitude larger. In this study, we aimed to extend optical visualization and resolve spatial heterogeneity throughout the entire tumour volume. We developed an optoacoustic (photoacoustic) mesoscope adapted to solid tumour imaging and, in a pilot study, offer the first insights into cancer optical contrast heterogeneity in vivo at an unprecedented resolution of <50 μm throughout the entire tumour mass. Using spectral methods, we resolve unknown patterns of oxygenation, vasculature and perfusion in three types of breast cancer and showcase different levels of structural and functional organization. To our knowledge, these results are the most detailed insights of optical signatures reported throughout entire tumours in vivo, and they position optoacoustic mesoscopy as a unique investigational tool linking microscopic and macroscopic observations.
Seasonality impels the antibiotic resistance in Kelani River of the emerging economy of Sri Lanka
Authors: Kumar Manish , Chaminda G. G. Tushara , Honda Ryo
Published: April 2020

We evaluated the occurrence of antibiotic-resistant bacteria, antibiotic-resistant gene, and metal concentration in a tropical river of Sri Lanka as a pre-emptive effort to understand the seasonal impact on their prevalence. Resistance for norfloxacin, ciprofloxacin, levofloxacin, kanamycin monosulfate (KM), tetracycline (TC), and sulfamethoxazole (ST) was measured with Kirby–Bauer disc diffusion method. The prevalence of Escherichia coli ranged from 10 to 27 CFU (colony-forming unit) ml−1 in Kelani River in Sri Lanka, and most of the E. coli isolates were resistant to more than one antibiotic. However, the resistance for TC and ST was much higher than other antibiotics. We found that the resistance percentage for older antibiotics like TC and ST was higher than the newer antibiotics. We detected genes that confer resistance to TCs, sulfonamides, β-lactams, and fluoroquinolones. Seasonal variation in the resistance of fluoroquinolones was much higher than the non-fluoroquinolones, but the effect was antagonistic. Overall, the significant seasonal variations imply the importance of mixed source and environmental conditions for development and transmission of antibiotic resistance.