bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of FOSS4G Supported Equal-Area Projections Using Discrete Distortion Indicatrices
Authors: Moreira de Sousa Luís , Poggio Laura , Kempen Bas
Published: August 2019

This study compares the performance of five popular equal-area projections supported by Free and Open Source Software for Geo-spatial (FOSS4G)—Sinusoidal, Mollweide, Hammer, Eckert IV and Homolosine. A set of 21,872 discrete distortion vindicatrices were positioned on the ellipsoid surface, centred on the cells of a Snyder icosahedral equal-area grid. These indicatrices were projected on the plane and the resulting angular and distance distortions computed, all using FOSS4G. The Homolosine is the only projection that manages to minimise angular and distance distortions simultaneously. It yields the lowest distortions among this set of projections and clearly outclasses when only land masses are considered. These results also indicate the Sinusoidal and Hammer projections to be largely outdated, imposing too large distortions to be useful. In contrast, the Mollweide and Eckert IV projections present trade-offs between visual expression and accuracy that are worth considering. However, for the purposes of storing and analysing big spatial data with FOSS4G the superior performance of the Homolosine projection makes its choice difficult to avoid.
Expression of Chemerin and Its Receptors in the Porcine Hypothalamus and Plasma Chemerin Levels during the Oestrous Cycle and Early Pregnancy
Authors: Smolinska Nina , Kiezun Marta , Dobrzyn Kamil , Rytelewska Edyta , Kisielewska Katarzyna , Gudelska Marlena , Zaobidna Ewa , Bogus-Nowakowska Krystyna , Wyrebek Joanna , Bors Kinga , Kopij Grzegorz , Kaminska Barbara , Kaminski Tadeusz
Published: August 2019

Chemerin (CHEM) may act as an important link integrating energy homeostasis and reproductive functions of females, and its actions are mediated by three receptors: chemokine-like receptor 1 (CMKLR1), G protein-coupled receptor 1 (GPR1), and C-C motif chemokine receptor-like 2 (CCRL2). The aim of the current study was to compare the expression of CHEM and its receptor (CHEM system) mRNAs (quantitative real-time PCR) and proteins (Western blotting and fluorescent immunohistochemistry) in the selected areas of the porcine hypothalamus responsible for gonadotropin-releasing hormone production and secretion: the mediobasal hypothalamus, preoptic area and stalk median eminence during the oestrous cycle and early pregnancy. Moreover, plasma CHEM concentrations were determined using ELISA. The expression of CHEM system has been demonstrated in the porcine hypothalamus throughout the luteal phase and follicular phase of the oestrous cycle, and during early pregnancy from days 10 to 28. Plasma CHEM levels and concentrations of transcripts and proteins of CHEM system components in the hypothalamus fluctuated throughout pregnancy and the oestrous cycle. Our study was the first experiment to demonstrate the presence of CHEM system mRNAs and proteins in the porcine hypothalamus and the correlations between the expression levels and physiological hormonal milieu related to the oestrous cycle and early pregnancy.
Vanin 1: Its Physiological Function and Role in Diseases
Authors: Bartucci Roberta , Salvati Anna , Olinga Peter , Boersma Ykelien L.
Published: August 2019

The enzyme vascular non-inflammatory molecule-1 (vanin 1) is highly expressed at gene and protein level in many organs, such as the liver, intestine, and kidney. Its major function is related to its pantetheinase activity; vanin 1 breaks down pantetheine in cysteamine and pantothenic acid, a precursor of coenzyme A. Indeed, its physiological role seems strictly related to coenzyme A metabolism, lipid metabolism, and energy production. In recent years, many studies have elucidated the role of vanin 1 under physiological conditions in relation to oxidative stress and inflammation. Vanin’s enzymatic activity was found to be of key importance in certain diseases, either for its protective effect or as a sensitizer, depending on the diseased organ. In this review, we discuss the role of vanin 1 in the liver, kidney, intestine, and lung under physiological as well as pathophysiological conditions. Thus, we provide a more complete understanding and overview of its complex function and contribution to some specific pathologies.
Experimental Study on Extreme Hydrodynamic Loading on Pipelines Part 2: Induced Force Analysis
Authors: Ghodoosipour Behnaz , Stolle Jacob , Nistor Ioan , Mohammadian Abdolmajid , Goseberg Nils
Published: August 2019

Adequate design of pipelines used for oil, gas, water, and wastewater transmission is essential not only for their proper operation but particularly to avoid failure and the possible extreme consequences. This is even more drastic in nearshore environments, where pipelines are potentially exposed to extreme hydrodynamic events, such as tsunami- or storm-surge-induced inundation. The American Society of Civil Engineers (ASCE), in its ASCE7 Chapter 6 on Tsunami Loads and Effects which is the new standard for tsunami impacts and loading, specifically stresses the need to study loads on pipelines located in tsunami-prone areas. To address this issue, this study is the first of its kind to investigate loading on pipelines due to tsunami-like bores. A comprehensive program of physical model experiments was conducted in the Dam-Break Hydraulic Flume at the University of Ottawa, Canada. The tests simulated on-land tsunami flow inundation propagating over a coastal plain. This allowed to record and investigate the hydrodynamic forces exerted on the pipe due to the tsunami-like, dam-break waves. Different pipe configurations, as well as various flow conditions, were tested to investigate their influence on exerted forces and moments. The goal of this study was to propose, based on the results of this study, resistance and lift coefficients which could be used for the design of pipelines located in tsunami-prone areas. The values of the resistance and lift coefficients investigated were found to be in the range of 1 <   C R < 3.5 and 0.5 ≤   C L < 3 , respectively. To that end, the study provides an upper envelope of resistance and lift coefficients over a wide range of Froude numbers for design purposes.
Low-Noise Mid-Infrared Photodetection in BP/h-BN/Graphene van der Waals Heterojunctions
Authors: Lu Qin , Yu Li , Liu Yan , Zhang Jincheng , Han Genquan , Hao Yue
Published: August 2019

We present a low-noise photodetector based on van der Waals stacked black phosphorus (BP)/boron nitride (h-BN)/graphene tunneling junctions. h-BN acts as a tunneling barrier that significantly blocks dark current fluctuations induced by shallow trap centers in BP. The device provides a high photodetection performance at mid-infrared (mid-IR) wavelengths. While it was found that the photoresponsivity is similar to that in a BP photo-transistor, the noise equivalent power and thus the specific detectivity are nearly two orders of magnitude better. These exemplify an attractive platform for practical applications of long wavelength photodetection, as well as provide a new strategy for controlling flicker noise.
Time-Consistent Strategies for the Generalized Multiperiod Mean-Variance Portfolio Optimization Considering Benchmark Orientation
Authors: Xiao Helu , Ren Tiantian , Zhou Zhongbao
Published: August 2019

In this paper, we propose a generalized multiperiod mean-variance portfolio optimization based on consideration of benchmark orientation and intertemporal restrictions, in which the investors not only focus on their own performance but also tend to compare the performance gap between themselves and the benchmark. We aim to find the time-consistent strategy under the generalized mean-variance criterion, such that their relative performance is maximized. We derive the time-consistent strategy for the proposed model with and without a risk-free asset by using the backward induction approach. The results show that, in the case that there exists a risk-free asset, the time-consistent strategy is a feedback strategy about the benchmark process. However, in the other case, the time-consistent strategy is a double feedback strategy on both the benchmark process and the wealth process. Finally, we carry out some numerical simulations to show the evolution process of the time-consistent strategy. These simulations indicate that the proposed strategy can not only reduce the risk of investment existed in the intermediate time period but also imitate the return of the benchmark process.
Iterative Methods for Solving a System of Linear Equations in a Bipolar Fuzzy Environment
Authors: Akram Muhammad , Muhammad Ghulam , Koam Ali N. A. , Hussain Nawab
Published: August 2019

We develop the solution procedures to solve the bipolar fuzzy linear system of equations (BFLSEs) with some iterative methods namely Richardson method, extrapolated Richardson (ER) method, Jacobi method, Jacobi over-relaxation (JOR) method, Gauss–Seidel (GS) method, extrapolated Gauss-Seidel (EGS) method and successive over-relaxation (SOR) method. Moreover, we discuss the properties of convergence of these iterative methods. By showing the validity of these methods, an example having exact solution is described. The numerical computation shows that the SOR method with ω = 1.25 is more accurate as compared to the other iterative methods.
Contemporary and Novel Imaging Studies for the Evaluation of Erectile Dysfunction
Authors: Chung Eric
Published: August 2019

Traditionally, it was thought that the pathogenesis of erectile dysfunction (ED) can be divided into psychological and organic factors. However, recent literature supports the development and progression of ED due to multidimensional alterations of a complex interplay of central and peripheral systems, from neural cognitive and efferent networks to loco-regional neuro-hormonal factors which are responsible for impaired penile vascular hemodynamics and ensuing lack of, or suboptimal, blood flow into the penis and/or veno-occlusive dysfunction. It is recognised that ED is strongly correlated with cardiovascular health and published clinical guidelines advocate screening for cardiovascular and metabolic risk factors in men presenting with ED. Over the past few decades, various imaging modalities have been developed and utilised to provide objective evaluation for ED to better characterise the state of penile health and exclude psychogenic components. The following article evaluates current and emerging imaging diagnostic tools for ED.
The Effect of Ultra-Fast Heating on the Microstructure, Grain Size and Texture Evolution of a Commercial Low-C, Medium-Mn DP Steel
Authors: Banis Alexandros , Hernandez Duran Eliseo , Bliznuk Vitaliy , Sabirov Ilchat , Petrov Roumen H. , Papaefthymiou Spyros
Published: August 2019

The effect of ultra-fast heating on the microstructures of steel has been thoroughly studied over the last year as it imposes a suitable alternative for the production of ultra high strength steel grades. Rapid reheating followed by quenching leads to fine-grained mixed microstructures. This way the desirable strength/ductility ratio can be achieved while the use of costly alloying elements is significantly reduced. The current work focuses on the effect of ultra-fast heating on commercial dual phase grades for use in the automotive industry. Here, a cold-rolled, low-carbon, medium-manganese steel was treated with a rapid heating rate of 780 °C/s to an intercritical peak temperature (760 °C), followed by subsequent quenching. For comparison, a conventionally heated sample was studied with a heating rate of 10 °C/s. The initial microstructure of both sets of samples consisted of ferrite, pearlite and martensite. It is found that the very short heating time impedes the dissolution of cementite and leads to an interface-controlled α → γ transformation. The undissolved cementite affects the grain size of the parent austenite grains and of the microstructural constituents after quenching. The final microstructure consists of ferrite and martensite in a 4/1 ratio, undissolved cementite and traces of austenite while the presence of bainite is possible. Finally, it is shown that the texture is not strongly affected during ultra-fast heating, and the recovery and recrystallization of ferrite are taking place simultaneously with the α → γ transformation.
Screening of the High-Rhizosphere Competent Limoniastrum monopetalum’ Culturable Endophyte Microbiota Allows the Recovery of Multifaceted and Versatile Biocontrol Agents
Authors: Ben Slama Houda , Triki Mohamed Ali , Chenari Bouket Ali , Ben Mefteh Fedia , Alenezi Faizah N. , Luptakova Lenka , Cherif-Silini Hafsa , Vallat Armelle , Oszako Tomasz , Gharsallah Neji , Belbahri Lassaad
Published: August 2019

Halophyte Limoniastrum monopetalum, an evergreen shrub inhabiting the Mediterranean region, has well-documented phytoremediation potential for metal removal from polluted sites. It is also considered to be a medicinal halophyte with potent activity against plant pathogens. Therefore, L. monopetalum may be a suitable candidate for isolating endophytic microbiota members that provide plant growth promotion (PGP) and resistance to abiotic stresses. Selected for biocontrol abilities, these endophytes may represent multifaceted and versatile biocontrol agents, combining pathogen biocontrol in addition to PGP and plant protection against abiotic stresses. In this study 117 root culturable bacterial endophytes, including Gram-positive (Bacillus and Brevibacillus), Gram-negative (Proteus, Providencia, Serratia, Pantoea, Klebsiella, Enterobacter and Pectobacterium) and actinomycete Nocardiopsis genera have been recovered from L. monopetalum. The collection exhibited high levels of biocontrol abilities against bacterial (Agrobacterium tumefaciens MAT2 and Pectobacterium carotovorum MAT3) and fungal (Alternaria alternata XSZJY-1, Rhizoctonia bataticola MAT1 and Fusarium oxysporum f. sp. radicis lycopersici FORL) pathogens. Several bacteria also showed PGP capacity and resistance to antibiotics and metals. A highly promising candidate Bacillus licheniformis LMRE 36 with high PGP, biocontrol, metal and antibiotic, resistance was subsequently tested in planta (potato and olive trees) for biocontrol of a collection of 14 highly damaging Fusarium species. LMRE 36 proved very effective against the collection in both species and against an emerging Fusarium sp. threatening olive trees culture in nurseries. These findings provide a demonstration of our pyramiding strategy. Our strategy was effective in combining desirable traits in biocontrol agents towards broad-spectrum resistance against pathogens and protection of crops from abiotic stresses. Stacking multiple desirable traits into a single biocontrol agent is achieved by first, careful selection of a host for endophytic microbiota recovery; second, stringent in vitro selection of candidates from the collection; and third, application of the selected biocontrol agents in planta experiments. That pyramiding strategy could be successfully used to mitigate effects of diverse biotic and abiotic stresses on plant growth and productivity. It is anticipated that the strategy will provide a new generation of biocontrol agents by targeting the microbiota of plants in hostile environments.
Metagenomic Sequencing Identifies Highly Diverse Assemblages of Dinoflagellate Cysts in Sediments from Ships’ Ballast Tanks
Authors: Shang Lixia , Hu Zhangxi , Deng Yunyan , Liu Yuyang , Zhai Xinyu , Chai Zhaoyang , Liu Xiaohan , Zhan Zifeng , Dobbs Fred C. , Tang Ying Zhong
Published: August 2019

Ships’ ballast tanks have long been known as vectors for the introduction of organisms. We applied next-generation sequencing to detect dinoflagellates (mainly as cysts) in 32 ballast tank sediments collected during 2001–2003 from ships entering the Great Lakes or Chesapeake Bay and subsequently archived. Seventy-three dinoflagellates were fully identified to species level by this metagenomic approach and single-cell polymerase chain reaction (PCR)-based sequencing, including 19 toxic species, 36 harmful algal bloom (HAB) forming species, 22 previously unreported as producing cysts, and 55 reported from ballast tank sediments for the first time (including 13 freshwater species), plus 545 operational taxonomic units (OTUs) not fully identified due to a lack of reference sequences, indicating tank sediments are repositories of many previously undocumented taxa. Analyses indicated great heterogeneity of species composition among samples from different sources. Light and scanning electron microscopy and single-cell PCR sequencing supported and confirmed results of the metagenomic approach. This study increases the number of fully identified dinoflagellate species from ballast tank sediments to 142 (>50% increase). From the perspective of ballast water management, the high diversity and spatiotemporal heterogeneity of dinoflagellates in ballast tanks argues for continuing research and stringent adherence to procedures intended to prevent unintended introduction of non-indigenous toxic and HAB-forming species.
Fusion of Lysostaphin to an Albumin Binding Domain Prolongs Its Half-Life and Bactericidal Activity in the Systemic Circulation
Authors: Grishin Alexander V. , Shestak Nikita V. , Lavrova Natalia V. , Lyashchuk Alexander M. , Popova Liubov I. , Strukova Natalia V. , Generalova Maria S. , Ryazanova Anna V. , Polyakov Nikita B. , Galushkina Zoya M. , Soboleva Lyubov A. , Boksha Irina S. , Karyagina Anna S. , Lunin Vladimir G.
Published: August 2019

Antibacterial lysins are promising proteins that are active against both antibiotic-susceptible and antibiotic-resistant bacterial strains. However, a major limitation of antibacterial lysins is their fast elimination from systemic circulation. PEGylation increases the plasma half-life of lysins but renders them inactive. Here we report the construction of a fusion protein of lysostaphin, a potent anti-staphylococcal lysin, and an albumin-binding domain from streptococcal protein G. The resulting fusion protein was less active than the parent enzyme lysostaphin, but it still retained significant antibacterial activity even when bound to serum albumin. The terminal half-life of the fusion protein in rats was five-fold greater than that of lysostaphin (7.4 vs. 1.5 h), and the area under the curve increased more than 115 times. Most importantly, this increase in systemic circulation time compensated for the decrease in activity. The plasma from rats that received an injection of the fusion protein retained bactericidal activity for up to 7 h, while plasma from rats that received plain lysostaphin lacked any detectable activity after 4 h. To the best of our knowledge, this is the first report of an antibacterial lysin with both improved pharmacokinetic parameters and prolonged bactericidal activity in the systemic circulation.
Study on Paramagnetic Interactions of (CH3NH3)2CoBr4 Hybrid Perovskites Based on Nuclear Magnetic Resonance (NMR) Relaxation Time
Authors: Lim Ae Ran , Kim Sun Ha
Published: August 2019

The thermal properties of organic–inorganic (CH3NH3)2CoBr4 crystals were investigated using differential scanning calorimetry and thermogravimetric analysis. The phase transition and partial decomposition temperatures were observed at 460 K and 572 K. Nuclear magnetic resonance (NMR) chemical shifts depend on the local field at the site of the resonating nucleus. In addition, temperature-dependent spin–lattice relaxation times (T1ρ) were measured using 1H and 13C magic angle spinning NMR to elucidate the paramagnetic interactions of the (CH3NH3)+ cations. The shortening of 1H and 13C T1ρ of the (CH3NH3)2CoBr4 crystals are due to the paramagnetic Co2+ effect. Moreover, the physical properties of (CH3NH3)2CoBr4 with paramagnetic ions and those of (CH3NH3)2CdBr4 without paramagnetic ions are reported and compared.
Determination of Various Drying Methods’ Impact on Odour Quality of True Lavender (Lavandula angustifolia Mill.) Flowers
Authors: Łyczko Jacek , Jałoszyński Klaudiusz , Surma Mariusz , García-Garví José Miguel , Carbonell-Barrachina Ángel A. , Szumny Antoni
Published: August 2019

True lavender flowers (Lavandula angustifolia Mill.) is a critical source of essential oils and a flavouring agent used in numerous industries like foods, cosmetics and pharmaceuticals. Its main volatile constituents are linalool and linalyl acetate, which are commonly considered as main odour-active constituents (OACs). Nevertheless, the quality of true lavender flowers is highly dependent on its post-harvest treatment, mainly the preservation method. Recognising that drying is the most frequently used preservation method, the influence of various drying methods, including convective drying (CD) at 50, 60 and 70 °C, vacuum-microwave drying (VMD) with powers 240, 360 and 480 W and combined convective pre-drying at 60 °C followed by vacuum-microwave finish-drying with power 480 W (CPD-VMFD), on the quality of true lavender flowers was verified. The evaluation of influence was carried out by HS-SPME(HS, solid-phase microextraction), GC-MS, GC-MS-O (gas chromatography–mass spectrometry–olfactometry) techniques. Moreover, the sensory panel has assessed the sample odour quality. As a result, the optimal drying methods regarding the requirements for products were established. Overall, for total essential oil recovery, CD at 50 °C is the optimal drying method, while for odour quality concerning the sensory panel evaluation, VMD with power 360 W combined CPD-VMFD and CD at 50 °C is the optimal drying method.
Biological Characterization and Inhibition of Streptococcus pyogenes ZUH1 Causing Chronic Cystitis by Crocus sativus Methanol Extract, Bee Honey Alone or in Combination with Antibiotics: An In Vitro Study
Authors: Abdel-Shafi Seham , Al-Mohammadi Abdul-Raouf , Hamdi Sara , Moustafa Ahmed H. , Enan Gamal
Published: August 2019

Streptococcus pyogenes (S. pyogenes) ZUH1 was isolated and characterized using morphological, cultural and biochemical methods. The results showed that the marker genes (namely spyCEP, ssa, sic, sdaB and speG) indicating group A streptococci (GAS) were detected in the S. pyogenes genome. The results showed that the S. pyogenes strain was inhibited by Crocus sativus methanol extract (CSME), bee honey (BH) and catfish glycoprotein (CFG). The inhibitory activity of these natural agents were compared with standard antibiotics such as Ceftazidime (30 μg/mL), Cefoperazone (75 μg/mL), Cefoxitin (30 μg/mL) and Imipenem (10 μg/mL). There was a synergistic effect between certain antibiotics and CSME. GC-MS and IR analysis of CSME showed different cyclic ketones, aldehydes, esters, alcohols and acids. The main compounds were tetradecanoic acid, safranal and isophorone. Transmission electron microscopy (TEM) images of S. pyogenes cells treated with CSME showed signs of an irregular wrinkled outer surface, fragmentation, adhesion and aggregation of damaged bacterial cells or cellular debris. The marker genes (spyCEP, ssa, sic, sdaB and speG) could be used as a rapid diagnostic tool for GAS. CSME, BH and CFG showed distinctive anti-streptococcal activity either alone or in combinations with antibiotics; their action on S. pyogenes cells was studied by TEM. There was a synergistic effect between antibiotics and Crocus sativus, bee honey, and glycoprotein against S. pyogenes ZUH1. The action of natural agents on the pathogenic cells was shown using TEM.
Recent Advances in the Synthesis of 2H-Pyrans
Authors: Tejedor David , Delgado-Hernández Samuel , Diana-Rivero Raquel , Díaz-Díaz Abián , García-Tellado Fernando
Published: August 2019

In this review, we discuss the nature of the different physicochemical factors affecting the valence isomerism between 2H-pyrans (2HPs) and 1-oxatrienes, and we describe the most versatile synthetic methods reported in recent literature to access to 2HPs, with the only exception of 2HPs fused to aromatic rings (i.e., 2H-chromenes), which are not included in this review.
Ultra-Porous Nanocellulose Foams: A Facile and Scalable Fabrication Approach
Authors: Antonini Carlo , Wu Tingting , Zimmermann Tanja , Kherbeche Abderrahmane , Thoraval Marie-Jean , Nyström Gustav , Geiger Thomas
Published: August 2019

Cellulose nanofibril foams are cellulose-based porous materials with outstanding mechanical properties, resulting from the high strength-to-weight ratio of nanofibrils. Here we report the development of an optimized fabrication process for highly porous cellulose foams, based on a well-controlled freeze-thawing-drying (FTD) process at ambient pressure. This process enables the fabrication of foams with ultra-high porosity, up to 99.4%, density of 10 mg/cm3, and liquid (such as oil) absorption capacity of 100 L/kg. The proposed approach is based on the ice-templating of nanocellulose suspension in water, followed by thawing in ethanol and drying at environmental pressures. As such, the proposed fabrication route overcomes one of the major bottle-necks of the classical freeze-drying approach, by eliminating the energy-demanding vacuum drying step required to avoid wet foam collapse upon drying. As a result, the process is simple, environmentally friendly, and easily scalable. Details of the foam development fabrication process and functionalization are thoroughly discussed, highlighting the main parameters affecting the process, e.g., the concentration of nanocellulose and additives used to control the ice nucleation. The foams are also characterized by mechanical tests and oil absorption measurements, which are used to assess the foam absorption capability as well as the foam porosity. Compound water-in-oil drop impact experiments are used to demonstrate the potential of immiscible liquid separation using cellulose foams.
Polyhedral Oligomeric Silsesquioxane (POSS) Surface Grafting: A Novel Method to Enhance Polylactide Hydrolysis Resistance
Authors: Li Kun , Colonna Samuele , Fina Alberto , Monticelli Orietta
Published: August 2019

This work considers the development of an easy and scalable approach to change the features of poly(l-lactide) (PLLA) films, which is based on the application of a surface treatment with an amino-functionalized polyhedral oligomeric silsesquioxane (POSS). Indeed, the developed approach is based on the potential reactivity of POSS amino group towards the polymer functionalities to produce an aminolysis reaction, which should promote the direct grafting of the silsesquioxane molecules on the polymer surface. Neat and treated films were studied by infrared spectroscopy and X-ray photoelectron spectroscopy, which proved the effectiveness of POSS grafting. Moreover, scanning electron microscopy measurements demonstrated the homogeneous distribution of Si on the film surface treated with the silsesquioxane. The influence of the film treatment on the surface wettability was evidenced by contact angle measurements. These findings demonstrated a relevant enhancement of the surface hydrophobicity, which increase turned out to depend on the conditions applied, as it increased by increasing the reaction temperature and the contact time. Finally, in order to evaluate the stability of neat and of the treated PLLA films the surface morphology of the samples treated with pH 7.4 buffer at 50 °C was studied.
Dietary Behavior and Physical Activity in Children and Adolescents
Authors: Hebestreit Antje , Bogl Leonie H.
Published: August 2019

In recent years, diet- and lifestyle-related disorders have become a major health threat in Europe and worldwide [...]
Pre-Conception Maternal Food Intake and the Association with Childhood Allergies
Authors: Grieger Jessica A. , Pelecanos Anita M. , Hurst Cameron , Tai Andrew , Clifton Vicki L.
Published: August 2019

Background: Periconceptional nutrition may have an important function in programming the immune function and allergies, however, there is a lack of studies assessing pre-conception food intake and childhood allergic disorders. The aim of the current study was to identify maternal pre-conception dietary components that may be associated with allergic disorders in children up to 3 years of age. Methods: Pregnant women attending their first antenatal visit and who were aged >18 years were invited to participate. Pre-conception food frequency data was retrospectively collected at 18 weeks’ gestation. Childhood eczema, current wheeze, and rhinitis was assessed at 36 months of age using a questionnaire and doctor diagnosis (n = 234). Linear discriminant analysis (LDA) was used to explore the combination of dietary food components that best discriminated between allergy status in children. Results: Maternal pre-conception food intake such as low and high fat dairy, fresh fruit, unsaturated spreads, and take-away foods, were protective for any allergy assessed. Non-oily fish was protective for eczema and current wheeze; saturated spreads (e.g., butter) was protective for eczema, current wheeze, and rhinitis; poultry and fruit juice were adversely associated with each allergy. Conclusions: Pre-conception food intakes demonstrate inconsistent and somewhat contrary relationships to the development of child allergies. Whether and how maternal food intake impacts the underlying fetal programming and the mechanisms of childhood allergy warrants further investigation.
Detection and Molecular Identification of Salmonella Virulence Genes in Livestock Production Systems in South Africa
Authors: Mthembu Thobeka P. , Zishiri Oliver T. , El Zowalaty Mohamed E.
Published: August 2019

Livestock are an important source of protein and food for humans, however opportunistic pathogens such as Salmonella spp. turn livestock into vehicles of foodborne diseases. This study investigated the prevalence of virulence genes in Salmonella spp. isolated from livestock production systems in two provinces of South Africa. During the period from May to August, 2018, a total of 361 faecal (189), oral (100), environmental (soil (36) and water (27)) and feed (9) samples were randomly collected from different animals (cattle, sheep, goats, pigs, ducks and chickens) that were housed in small-scale livestock production systems from Eastern Cape and KwaZulu-Natal Provinces in South Africa. Salmonella spp. were isolated and identified using microbiological and DNA molecular methods. Salmonella spp. were present in 29.0% of the samples of which 30.2% belonged to the Salmonella enterica species as confirmed by the positive amplification of the species specific iroB gene. Virulence genes that were screened from livestock-associated Salmonella were invA, iroB, spiC, pipD and int1. Statistically significant associations (p < 0.05) were established between the virulence genes, sampling location, animal host as well as the season when samples were collected. Furthermore, statistically significant (p < 0.05) positive correlations were observed between most of the virulence genes investigated. This is one of the recent studies to detect and investigate livestock-associated Salmonella spp. in South Africa. This study highlights the importance of continuous monitoring and surveillance for pathogenic salmonellae. It also demonstrated the detection and prevalence of virulent Salmonella spp. harbored by livestock in South Africa. This study demonstrated the potential risks of pathogenic Salmonella enterica to cause foodborne diseases and zoonotic infections from farm-to-fork continuum using the global one-health approach.
A Cross-Sectional Investigation for Verification of Globalization of Falsified Medicines in Cambodia, Indicated by Tablets of Sildenafil Citrate
Authors: Yoshida Naoko , Yuasa Miku , Sovannarith Tey , Dararth Eav , Keila Tep , Kiet Heng Bun , Tsuboi Hirohito , Tanimoto Tsuyoshi , Kimura Kazuko
Published: August 2019

Medicine falsification is a global issue. Viagra, an erectile dysfunction therapeutic (EDT) medicine consisting primarily of sildenafil citrate, is the most commonly falsified medicine worldwide. Recently falsified EDTs have been reported multiple times in developing countries. The globalization of falsified EDTs has become a concern. In the present study, we selected sildenafil citrate tablets as an indicator and examined samples from a developing country, Cambodia, to investigate the availability of falsified sildenafil tablets in Cambodia and verify the current globalization status of falsified medicines from the standpoint of a developing country. Six samples of the originator Viagra, and 68 samples of generic sildenafil products were purchased from private drug outlets and wholesalers in Phnom Penh, Svay Rieng, and Battambang. The samples’ manufacturers were contacted to authenticate the samples. The quantities and dissolution rates of active ingredients were measured by a high-performance liquid chromatography system with photodiode array. Five generic samples were strongly suspected to be falsified medicines because of their extremely low quality; however, there was little distribution and no falsified medicine alleged to be produced by the originator of Viagra, which charges high prices. That finding indicates that falsification reflects local economic circumstances.
Long-Term Maintainable Somatic Embryogenesis System in Alfalfa (Medicago sativa) Using Leaf Explants: Embryogenic Sustainability Approach
Authors: Sangra Ankush , Shahin Lubana , Dhir Sarwan K.
Published: August 2019

Alfalfa (Medicago sativa) is one of the most important forage legume crops because of its mass production and high feeding value. It originated in Asia and is one of the most ancient plants cultivated throughout the world as a fodder. Despite the well-studied somatic embryogenesis of alfalfa, there is a lack of a long-term maintainable somatic embryogenic system. Every time an embryogenic callus culture must be started from new explants, which is laborious, costly and time consuming. In addition to this, endogenous microorganisms present in ex vitro explants of alfalfa can often cause contamination, reducing the efficiency of callus culture. An attempt was made to establish long-term continuous somatic embryogenesis system in alfalfa using cultivar Regen-SY. Nine somatic embryogenesis pathways were studied and evaluated for embryo yield, plant conversion rate and embryogenic sustainability. Somatic embryos passed through the same stages (globular, heart-shaped, torpedo and cotyledonary) as characteristic of the zygotic embryo and secondary somatic embryogenesis was also observed. B5H-B5 system showed the highest embryo yield and plant conversion rate whereas SH4K-BOi2Y system demonstrated the highest embryogenic sustainability and maintained the embryogenic potential even after six subculture cycles. Scanning electron microscopy was applied to study the morphology of the somatic embryos and secondary somatic embryogenesis. Therefore, long-term maintainable somatic embryogenesis system protocol was developed through this study, which will help to enhance and accelerate the alfalfa biotechnology research.
Numerical Simulation of Effects of Different Operational Parameters on the Carbon Solution Loss Ratio of Coke inside Blast Furnace
Authors: Kou Mingyin , Zhou Heng , Wang Li Pang , Hong Zhibin , Yao Shun , Xu Haifa , Wu Shengli
Published: August 2019

Carbon solution loss reaction of coke gasification is one of the most important reasons for coke deterioration and degradation in a blast furnace. It also affects the permeability of gas and fluids, as well as stable working conditions. In this paper, a three dimensional model is established based on the operational parameters of blast furnace B in Bayi Steel. The model is then used to calculate the effects of oxygen enrichment, coke oven gas injection, and steel scrap charging on the carbon solution loss ratio of coke in the blast furnace. Results show that the carbon solution loss ratio of coke gasification for blast furnace B is almost 20% since the results of a model are probably only indicative. The oxygen enrichment and the addition of steel scrap can reduce the carbon solution loss ratio with little effect on the working condition. However, coke oven gas injection increases the carbon solution loss ratio. Therefore, coke oven gas should not be injected into the blast furnace unless the quality of the coke is improved.
Erratum: Arfan M., et al. Assessment of Spatial and Temporal Flow Variability of the Indus River. Resources 2019, 8, 103
Authors: Arfan Muhammad , Lund Jewell , Hassan Daniyal , Saleem Maaz , Ahmed Aftab
Published: August 2019

The authors wish to make the following corrections to the published paper [...]
A Simulation Environment for Validation and Verification of Real Time Hyperspectral Processing Algorithms on-Board a UAV
Authors: Horstrand Pablo , López José Fco. , López Sebastián , Leppälampi Tapio , Pusenius Markku , Rooker Martijn
Published: August 2019

The utilization of hyperspectral imaging sensors has gained a significant relevance among many different applications due to their capability for collecting a huge amount of information across the electromagnetic spectrum. These sensors have been traditionally mounted on-board satellites and airplanes in order to extract information from the Earth’s surface. Fortunately, the progressive miniaturization of these sensors during the last lustrum has enabled their use in other remote sensing platforms, such as drones equipped with hyperspectral cameras which bring advantages in terms of higher spatial resolution of the acquired images, more flexible revisit times and lower cost of the flight campaigns. However, when these drones are autonomously flying and taking real-time critical decisions from the information contained in the captured images, it is crucial that the whole process takes place in a safe and predictable manner. In order to deal with this problem, a simulation environment is presented in this work to analyze the virtual behavior of a drone equipped with a pushbroom hyperspectral camera used for assisting harvesting applications, which enables an exhaustive and realistic validation and verification of the drone real-time hyperspectral imaging system prior to its launch. To the best of the authors’ knowledge, the proposed environment represents the only solution in the state-of-the-art that allows the virtual verification of real-time hyperspectral image processing algorithms under realistic conditions.
Remotely Sensed Water Limitation in Vegetation: Insights from an Experiment with Unmanned Aerial Vehicles (UAVs)
Authors: Easterday Kelly , Kislik Chippie , Dawson Todd , Hogan Sean , Kelly Maggi
Published: August 2019

Unmanned aerial vehicles (UAVs) equipped with multispectral sensors present an opportunity to monitor vegetation with on-demand high spatial and temporal resolution. In this study we use multispectral imagery from quadcopter UAVs to monitor the progression of a water manipulation experiment on a common shrub, Baccharis pilularis (coyote brush) at the Blue Oak Ranch Reserve (BORR) ~20 km east of San Jose, California. We recorded multispectral imagery at several altitudes with nearly hourly intervals to explore the relationship between two common spectral indices, NDVI (normalized difference vegetation index) and NDRE (normalized difference red edge index), leaf water content and water potential as physiological metrics of plant water status, across a gradient of water deficit. An examination of the spatial and temporal thresholds at which water limitations were most detectable revealed that the best separation between levels of water deficit were at higher resolution (lower flying height), and in the morning (NDVI) and early morning (NDRE). We found that both measures were able to identify moisture deficit across treatments; however, NDVI was better able to distinguish between treatments than NDRE and was more positively correlated with field measurements of leaf water content. Finally, we explored how relationships between spectral indices and water status changed when the imagery was scaled to courser resolutions provided by satellite-based imagery (PlanetScope).We found that PlanetScope data was able to capture the overall trend in treatments but unable to capture subtle changes in water content. These kinds of experiments that evaluate the relationship between direct field measurements and UAV camera sensitivity are needed to enable translation of field-based physiology measurements to landscape or regional scales.
Detection of Strong NOX Emissions from Fine-scale Reconstruction of the OMI Tropospheric NO2 Product
Authors: Lee Jae-Hyeong , Lee Sang-Hyun , Kim Hyun Cheol
Published: August 2019

Satellite-retrieved atmospheric NO2 column products have been widely used in assessing bottom-up NOX inventory emissions emitted from large cities, industrial facilities, and power plants. However, the satellite products fail to quantify strong NOX emissions emitted from the sources less than the satellite’s pixel size, with significantly underestimating their emission intensities (smoothing effect). The poor monitoring of the emissions makes it difficult to enforce pollution restriction regulations. This study reconstructs the tropospheric NO2 vertical column density (VCD) of the Ozone Monitoring Instrument (OMI)/Aura (13 × 24 km2 pixel resolution at nadir) over South Korea to a fine-scale product (grid resolution of 3 × 3 km2) using a conservative spatial downscaling method, and investigates the methodological fidelity in quantifying the major Korean area and point sources that are smaller than the satellite’s pixel size. Multiple high-fidelity air quality models of the Weather Research and Forecast-Chemistry (WRF-Chem) and the Weather Research and Forecast/Community Multiscale Air Quality modeling system (WRF/CMAQ) were used to investigate the downscaling uncertainty in a spatial-weight kernel estimate. The analysis results showed that the fine-scale reconstructed OMI NO2 VCD revealed the strong NOX emission sources with increasing the atmospheric NO2 column concentration and enhanced their spatial concentration gradients near the sources, which was accomplished by applying high-resolution modeled spatial-weight kernels to the original OMI NO2 product. The downscaling uncertainty of the reconstructed OMI NO2 product was inherent and estimated by 11.1% ± 10.6% at the whole grid cells over South Korea. The smoothing effect of the original OMI NO2 product was estimated by 31.7% ± 13.1% for the 6 urbanized area sources and 32.2% ± 17.1% for the 13 isolated point sources on an effective spatial resolution that is defined to reduce the downscaling uncertainty. Finally, it was found that the new reconstructed OMI NO2 product had a potential capability in quantifying NOX emission intensities of the isolated strong point sources with a good correlation of R = 0.87, whereas the original OMI NO2 product failed not only to identify the point sources, but also to quantify their emission intensities (R = 0.30). Our findings highlight a potential capability of the fine-scale reconstructed OMI NO2 product in detecting directly strong NOX emissions, and emphasize the inherent methodological uncertainty in interpreting the reconstructed satellite product at a high-resolution grid scale.
Optimizing 802.15.4 Outdoor IoT Sensor Networks for Aerial Data Collection
Authors: Nekrasov Michael , Allen Ryan , Artamonova Irina , Belding Elizabeth
Published: August 2019

Rural IoT sensor networks, prevalent in environmental monitoring and precision agriculture, commonly operate over some variant of the IEEE 802.15.4 standard. Data collection from these networks is often challenging, as they may be deployed in remote regions where existing backhaul infrastructure is expensive or absent. With the commercial and industrial success of Unmanned Aircraft Systems (UAS), there is understandable interest in using UASs for delay tolerant data collection from 802.15.4 IoT sensor networks. In this study, we investigate how to optimize 802.15.4 networks for aerial data collection, which, unlike other wireless standards, has not received rigorous evaluation for three-dimensional aerial communication. We analyze experimental measurements from an outdoor aerial testbed, examining how factors, such as antenna orientation, altitude, antenna placement, and obstruction, affect signal strength and packet reception rate. In our analysis, we model and predict the quality of service for aerial data collection, based on these network configuration variables, and contrast that with the Received Signal Strength Indication (RSSI)—a commonly used signal strength metric. We find that network configuration plays a significant role in network quality, which RSSI, a mediator variable, struggles to account for in the presence of high packet loss. We conclude with a discussion of strategies for optimizing sensor network configuration for aerial data collection, in light of our results.
New Data on the Messapian Necropolis of Monte D’Elia in Alezio (Apulia, Italy) from Topographical and Geophysical Surveys
Authors: Leucci Giovanni , Giorgi Lara De , Ditaranto Immacolata , Giuri Francesco , Ferrari Ivan , Scardozzi Giuseppe
Published: August 2019

The Messapian necropolis of Monte D’Elia is related to one of the most important ancient settlements in the Salento Peninsula (in south Italy). In order to understand the extension and layout of this necropolis in the various periods of its use, a ground-penetrating radar (GPR) prospection was undertaken in some important sample areas by a team of the Institute for Archaeological and Monumental Heritage of the National Research Council of Italy. The analysis of the GPR measurements revealed many anomalies that could be ascribed to archaeological structures (tombs), as well as other anomalies of presumable natural origin or referable to modern features. The data collected were georeferenced in the digital archaeological map of the site and integrated with a virtual reconstruction of the surveyed area.
An Enhanced Virtual Force Algorithm for Diverse k-Coverage Deployment of 3D Underwater Wireless Sensor Networks
Authors: Wang Wenming , Huang Haiping , He Fan , Xiao Fu , Jiang Xin , Sha Chao
Published: August 2019

The combination of Wireless Sensor Networks (WSNs) and edge computing not only enhances their capabilities, but also motivates a series of new applications. As a typical application, 3D Underwater Wireless Sensor Networks (UWSNs) have become a hot research issue. However, the coverage of underwater sensor networks problem must be solved, for it has a great significance for the network’s capacity for information acquisition and environment perception, as well as its survivability. In this paper, we firstly study the minimal number of sensor nodes needed to build a diverse k-coverage sensor network. We then propose a k-Equivalent Radius enhanced Virtual Force Algorithm (called k-ERVFA) to achieve an uneven regional coverage optimization for different k-coverage requirements. Theoretical analysis and simulation experiments are carried out to demonstrate the effectiveness of our proposed algorithm. The detailed performance comparisons show that k-ERVFA acquires a better coverage rate in high k-coverage sub-regions, thus achieving a desirable diverse k-coverage deployment. Finally, we perform sensitivity analysis of the simulation parameters and extend k-ERVFA to special cases such as sensor-sparse regions and time-variant situations.