bluuebunny/crossref_metadata_2025 huggingface dataset:
A Modified Version of the RNG k–ε Turbulence Model for the Scale-Resolving Simulation of Internal Combustion Engines
Authors: Krastev Vesselin , Silvestri Luca , Falcucci Giacomo
Published: December 2017

The unsteady and random character of turbulent flow motion is a key aspect of the multidimensional modeling of internal combustion engines (ICEs). A typical example can be found in the prediction of the cycle-to-cycle variability (CCV) in modern, highly downsized gasoline direct injection (GDI) engines, which strongly depends on the accurate simulation of turbulent in-cylinder flow structures. The current standard for turbulence modeling in ICEs is still represented by the unsteady form of Reynold-averaged Navier Stokes equations (URANS), which allows the simulation of full engine cycles at relatively low computational costs. URANS-based methods, however, are only able to return a statistical description of turbulence, as the effects of all scales of motion are entirely modeled. Therefore, during the last decade, scale-resolving methods such as large eddy simulation (LES) or hybrid URANS/LES approaches are gaining increasing attention among the engine-modeling community. In the present paper, we propose a scale-resolving capable modification of the popular RNG k– ε URANS model. The modification is based on a detached-eddy simulation (DES) framework and allows one to explicitly set the behavior (URANS, DES or LES) of the model in different zones of the computational domain. The resulting zonal formulation has been tested on two reference test cases, comparing the numerical predictions with the available experimental data sets and with previous computational studies. Overall, the scale-resolved part of the computed flow has been found to be consistent with the expected flow physics, thus confirming the validity of the proposed simulation methodology.
Environmental Impact of End-of-Life Tires: Life Cycle Assessment Comparison of Three Scenarios from a Case Study in Valle Del Cauca, Colombia
Authors: Ortíz-Rodríguez Oscar , Ocampo-Duque William , Duque-Salazar Laura
Published: December 2017

Life Cycle Assessment methodology has been applied to estimate diverse environmental impacts of different usage alternatives for worn-out tires at the end of their useful life in a case study at the Department of Valle del Cauca, Colombia. Different real scenarios were compared, which allowed for the assessment of the best environmental option for the management of worn-out tires. A method developed in the Institute of Environmental Sciences at University of Leiden, better known as CML-2001, was used to calculate the environmental impact indicators. The results show that the incineration of whole tires in cement plants, and the activities of grinding and floor manufacturing from granulated rubber, exhibited the best indicators, especially in terms of environmental load avoidance through the recovery of materials. Finally, the categories of depletion of the ozone layer, acidification, global warming potential, depletion of abiotic resources, and photochemical ozone formation revealed that the strongest environmental impacts are associated with retreading and the production of multipart asphalt. This is due to the use of synthetic rubber in the former alternative, and of liquid asphalt, gravel, and diesel consumption in the latter.
Chip Temperature-Based Workload Allocation for Holistic Power Minimization in Air-Cooled Data Center
Authors: Bai Yan , Gu Lijun
Published: December 2017

Minimizing the energy consumption is a dominant problem in data center design and operation. To cope with this issue, the common approach is to optimize the data center layout and the workload distribution among servers. Previous works have mainly adopted the temperature at the server inlet as the optimization constraint. However, the inlet temperature does not properly characterize the server’s thermal state. In this paper, a chip temperature-based workload allocation strategy (CTWA-MTP) is proposed to reduce the holistic power consumption in data centers. Our method adopts an abstract heat-flow model to describe the thermal environment in data centers and uses a thermal resistance model to describe the convective heat transfer of the server. The core optimizes the workload allocation with respect to the chip temperature threshold. In addition, the temperature-dependent leakage power of the server has been considered in our model. The proposed method is described as a constrained nonlinear optimization problem to find the optimal solution by a genetic algorithm (GA). We applied the method to a sample data center constructed with computational fluid dynamics (CFD) software. By comparing the simulation results with other different workload allocation strategies, the proposed method prevents the servers from overcooling and achieves a substantial energy saving by optimizing the workload allocation in an air-cooled data center.
Sliding Surface in Consensus Problem of Multi-Agent Rigid Manipulators with Neural Network Controller
Authors: Nguyen Trong Thang , Nguyen Duc Minh
Published: December 2017

Based on Lyapunov theory, this research demonstrates the stability of the sliding surface in the consensus problem of multi-agent systems. Each agent in this system is represented by the dynamically uncertain robot, unstructured disturbances, and nonlinear friction, especially when the dynamic function of agent is unknown. All system states use neural network online weight tuning algorithms to compensate for the disturbance and uncertainty. Each agent in the system has a different position, and their trajectory approach to the same target is from each distinct orientation. In this research, we analyze the design of the sliding surface for this model and demonstrate which type of sliding surface is the best for the consensus problem. Lastly, simulation results are presented to certify the correctness and the effectiveness of the proposed control method.
Seven Operation Modes and Simulation Models of Solar Heating System with PCM Storage Tank
Authors: Zhao Juan , Ji Yasheng , Yuan Yanping , Zhang Zhaoli , Lu Jun
Published: December 2017

A physical model and dynamic simulation models of a solar phase-change heat storage heating system with a plate solar collector, phase-change material (PCM) storage tank, plate heat exchanger, and auxiliary heat sources were established. A control strategy and numerical models for each of seven different operation modes that cover the entire heating season of the system were developed for the first time. The seven proposed operation modes are Mode 1: free cooling; Mode 2: reservation of heat absorbed by the solar collector in the PCM storage tank when there is no heating demand; Mode 3: direct supply of the heating demand by the solar collector; Mode 4: use of the heat absorbed by the solar collector to meet the heating demands, with the excess heat stored in the PCM storage tank; Mode 5: use of heat stored in the PCM storage tank to meet the heating demands, Mode 6: combined use of heat stored in the PCM storage tank and the auxiliary heating sources to meet the heating demands; and Mode 7: exclusive use of the auxiliary heat sources in order to meet the heating demands. Mathematical models were established for each of the above seven operation modes, taking into consideration the effects of the outdoor meteorological parameters and terminal load on the heating system. The real-time parameters for the entire heating season of the system with respect to the different operation modes can be obtained by solving the simulation models, and used as reference for the optimal design and operation of the actual system.
Comparative Simulation of Various Agricultural Land Use Practices for Analysis of Impacts on Environments
Authors: Badenko Vladimir , Badenko Galina , Topaj Alex , Medvedev Sergey , Zakharova Elena , Terleev Vitaly
Published: December 2017

Current intensification and changes in agricultural land use practices increase environmental impact that can be reduced by bridging the gap between socio-economic demands and scientific justification of sustainable agricultural land use. This can be achieved by replacing the goal of maximum crop yields with the goal of minimal environmental impact. This paper presents results of integrated crop simulation system development for analysis of alternative planning strategies in agricultural land use, with focus on the crop rotation influence on environmental sustainability. The effective tools used in analysis include (1) long-term analysis of changes in agricultural land using a dynamic crop model with daily time step; (2) justification of arbitrary crop rotation scheme of different agro-technologies and sparing measures; and (3) analysis of modern farming management methods using model-oriented approach. The results of study also include estimation of two alternative practices of crop harvesting including remaining or removing whole crop residues from the agricultural field and their influence on basic parameters of soil fertility. In addition, we analyzed comparative efficiency of different agricultural measures neglecting the negative influence of possible climate changes in long-term consequences. Corresponding efficiency rating is the following: organic fertilizer, green manure legume sparing harvesting, winter catch crop, and rotation scheme.
Community Earth System Model Simulations Reveal the Relative Importance of Afforestation and Forest Management to Surface Temperature in Eastern North America
Authors: Ahlswede Benjamin , Thomas R.
Published: December 2017

Afforestation changes the land surface energy balance, though the effects on climate in temperate regions is uncertain, particularly the changes associated with forest management. In this study, we used idealized Community Earth System Model simulations to assess the influence of afforestation and afforestation management in eastern North America on climate via changes in the biophysics of the land surface. Afforestation using broadleaf deciduous trees maintained at high leaf area index (LAI) in the southern part of the study region provided the greatest climate benefit by cooling summer surface air temperatures (Tsa). In contrast, the greatest warming occurred in the northern extent of the study region when afforesting with needleleaf evergreen trees maintained at high LAI. Forest management had an equal or greater influence on Tsa than the overall decision to afforest land in the southern extent of the region. Afforestation had a greater influence on Tsa than forest management in the northern extent. Integrating our results, focused on biophysical processes, with other research quantifying carbon cycle sensitivity to management can help guide the use of temperate afforestation to optimize climate benefits. Further, our results highlight the potential importance of including forest management in simulations of past and future climate.
Combined Analysis of mRNAs and miRNAs to Identify Genes Related to Biological Characteristics of Autotetraploid Paulownia
Authors: Cao Xibing , Xu Enkai , Zhai Xiaoqiao , Dong Yanpeng , Fan Guoqiang
Published: December 2017

Autopolyploid plants and their organs are larger than their corresponding diploid ancestors, and they attract considerable attention for plant breeding. Paulownia is a fast-growing tree. To identify genes related to the biological characteristics of tetraploid Paulownia, transcriptome and small RNA sequencing were used to identify the key gene expression regulation in tetraploid Paulownia fortunei and tetraploid P. tomentosa and their corresponding diploids. A total of 1977 common differentially expressed genes (DEGs) and 89 differentially expressed miRNAs (DEMs) (38 conserved and 51 novel) were obtained in tetraploid vs. diploid comparisons of the two Paulownia species, and 18 target genes were identified by target prediction. Finally, by analyzing the expression profiles of the DEGs and DEMs and their target genes, we discovered that Pau-miR169, Pau-miR408 and Pau-miR156 interacted with their target gene nuclear transcription factor Y subunit A-9 (NF-YA9), serine/threonine protein phosphatase (PP1) and s-adenosyl-methionine-sterol-c-methyltransfera—se (SAM:SMT) to regulate the abiotic stress tolerance and the timber quality of the tetraploid Paulownia. This study lays a molecular biology foundation for understanding autotetraploid Paulownia and will benefit future breeding work.
NAD1 Controls Defense-Like Responses in Medicago truncatula Symbiotic Nitrogen Fixing Nodules Following Rhizobial Colonization in a BacA-Independent Manner
Authors: Domonkos Ágota , Kovács Szilárd , Gombár Anikó , Kiss Ernő , Horváth Beatrix , Kováts Gyöngyi , Farkas Attila , Tóth Mónika , Ayaydin Ferhan , Bóka Károly , Fodor Lili , Ratet Pascal , Kereszt Attila , Endre Gabriella , Kaló Péter
Published: December 2017

Legumes form endosymbiotic interaction with host compatible rhizobia, resulting in the development of nitrogen-fixing root nodules. Within symbiotic nodules, rhizobia are intracellularly accommodated in plant-derived membrane compartments, termed symbiosomes. In mature nodule, the massively colonized cells tolerate the existence of rhizobia without manifestation of visible defense responses, indicating the suppression of plant immunity in the nodule in the favur of the symbiotic partner. Medicago truncatula DNF2 (defective in nitrogen fixation 2) and NAD1 (nodules with activated defense 1) genes are essential for the control of plant defense during the colonization of the nitrogen-fixing nodule and are required for bacteroid persistence. The previously identified nodule-specific NAD1 gene encodes a protein of unknown function. Herein, we present the analysis of novel NAD1 mutant alleles to better understand the function of NAD1 in the repression of immune responses in symbiotic nodules. By exploiting the advantage of plant double and rhizobial mutants defective in establishing nitrogen-fixing symbiotic interaction, we show that NAD1 functions following the release of rhizobia from the infection threads and colonization of nodule cells. The suppression of plant defense is self-dependent of the differentiation status of the rhizobia. The corresponding phenotype of nad1 and dnf2 mutants and the similarity in the induction of defense-associated genes in both mutants suggest that NAD1 and DNF2 operate close together in the same pathway controlling defense responses in symbiotic nodules.
Measuring Activity Performance of Older Adults Using the activPAL: A Rapid Review
Authors: Chan Charice , Slaughter Susan , Jones C. , Ickert Carla , Wagg Adrian
Published: December 2017

Current measures of physical activity and sedentary behaviors such as questionnaires and functional assessments are insufficient to provide comprehensive data on older adults. In response, the use of activity monitors has increased. The purpose of this review was to summarize and assess the quality of observational literature on activity measuring of older adults using the activPAL activity monitor. Seventeen databases and a bibliography, compiled by the activPAL creators, were searched. Articles were included if they were in English, were peer-reviewed, included people 65 years or older, measured activity using the activPAL and reported at least one of the following outcomes: step count, hours upright, hours sitting/lying, hours stepping, or hours standing. The search revealed 404 titles; after exclusions 24 were included in the final review. Of these studies, one examined older adults from residential aged care, six from hospital in-patient clinics, nine from outpatient clinics and eight examined community-dwellers. Mean age ranged from 66.0 to 84.2 years. Not all studies reported similar outcome variables, preventing data pooling. The review found a lack of high quality articles. There may be limitations to using the activPAL among older adults but further research is required to examine its use in this population.
The Multi-Attribute Group Decision-Making Method Based on Interval Grey Trapezoid Fuzzy Linguistic Variables
Authors: Yin Kedong , Wang Pengyu , Li Xuemei
Published: December 2017

With respect to multi-attribute group decision-making (MAGDM) problems, where attribute values take the form of interval grey trapezoid fuzzy linguistic variables (IGTFLVs) and the weights (including expert and attribute weight) are unknown, improved grey relational MAGDM methods are proposed. First, the concept of IGTFLV, the operational rules, the distance between IGTFLVs, and the projection formula between the two IGTFLV vectors are defined. Second, the expert weights are determined by using the maximum proximity method based on the projection values between the IGTFLV vectors. The attribute weights are determined by the maximum deviation method and the priorities of alternatives are determined by improved grey relational analysis. Finally, an example is given to prove the effectiveness of the proposed method and the flexibility of IGTFLV.
Beyond Disaster Preparedness: Building a Resilience-Oriented Workforce for the Future
Authors: Madrigano Jaime , Chandra Anita , Costigan Tracy , Acosta Joie
Published: December 2017

Enhancing citizens’ and communities’ resilience is critical to adapt successfully to ongoing challenges faced by communities, as well as acute shocks resulting from disasters. While significant progress has been made in this area, several research and practice gaps remain. A crucial next step to advance resilience is the development of a resilience-oriented workforce. This narrative review examines existing literature to determine key components of a resilience-oriented workforce, with a focus on organizational structures, training and education, and leadership models. Reviewed articles spanned a variety of study types, including needs assessments of existing workforce, program evaluations, and reviews/commentaries. A resilience-oriented workforce spans many disciplines and training programs will need to reflect that. It requires a collaborative organizational model that promotes information sharing structures. Leadership models should foster a balance between workforce autonomy and operation as a collective entity. Optimal strategies to develop a resilience-oriented workforce have yet to be realized and future research will need to collect and synthesize data to promote and evaluate the growth of this field.
Airborne or Fomite Transmission for Norovirus? A Case Study Revisited
Authors: Xiao Shenglan , Tang Julian , Li Yuguo
Published: December 2017

Norovirus infection, a highly prevalent condition associated with a high rate of morbidity, comprises a significant health issue. Although norovirus transmission mainly occurs via the fecal-oral and vomit-oral routes, airborne transmission has been proposed in recent decades. This paper re-examines a previously described norovirus outbreak in a hotel restaurant wherein airborne transmission was originally inferred. Specifically, the original evidence that suggested airborne transmission was re-analyzed by exploring an alternative hypothesis: could this outbreak instead have occurred via fomite transmission? This re-analysis was based on whether fomite transmission could have yielded similar attack rate distribution patterns. Seven representative serving pathways used by waiters were considered, and the infection risk distributions of the alternative fomite transmission routes were predicted using a multi-agent model. These distributions were compared to the reported attack rate distribution in the original study using a least square methods approach. The results show that with some reasonable assumptions of human behavior patterns and parameter values, the attack rate distribution corresponded well with that of the infection risk via the fomite route. This finding offers an alternative interpretation of the transmission routes that underlay this particular norovirus outbreak and an important consideration in the development of infection control guidelines and the investigation of similar norovirus outbreaks in future.
Pericardial Effusion as a Presenting Symptom of Hashimoto Thyroiditis: A Case Report
Authors: Leonardi Alberto , Penta Laura , Cofini Marta , Lanciotti Lucia , Principi Nicola , Esposito Susanna
Published: December 2017

Background: Hashimoto thyroiditis (HT) is the most frequent cause of acquired hypothyroidism in paediatrics. HT is usually diagnosed in older children and adolescents, mainly in females and is rare in infants and toddlers with cardiac involvement, including pericardial effusion, that can be found in 10% to 30% of adult HT cases. In this paper, a child with HT and pericardial effusion as the most important sign of HT is described. Case presentation: A four-year-old male child suffering for a few months from recurrent abdominal pain sometimes associated with vomiting underwent an abdominal ultrasound scan outside the hospital. This led to the identification of a significant pericardial effusion. At admission, his family history revealed that both his mother and maternal grandmother suffered from HT and that both were treated with l-thyroxine (LT4). The clinical examination did not reveal any pathological signs other than a palpable thyroid. His weight was 21 kg (78th percentile), his height was 101.8 cm (12th percentile) and his body max index (BMI) was 20.26 (96th percentile). On a chest radiograph, his heart had a globular appearance and the lung fields were normal. An echocardiography confirmed and determined the effusion amount (max, 23 mm; 600 mL) with light impairment of the heart kinetics. The ECG showed sinus bradycardia with a normal ST tract. Based on the blood test results, an infectious cause of the pericardial fluid excess was considered unlikely. Thyroid function testing revealed very high thyrotropin (TSH, 487 μIU/mL; normal range, 0.340–5.600 μIU/mL) and low serum-free thyroxine (fT4, 0.04 ng/dL; normal range, 0.54–1.24 ng/dL) levels. High thyroid peroxidase antibody titres in the blood were evidenced (>1500 UI/L; normal values, 0.0–9.0 UI/L). The thyroid ultrasound was consistent with thyroiditis. HT was diagnosed, and LT4 replacement therapy with levothyroxine sodium 1.78 µg/kg/die was initiated, with a gradual increase of the administered dose. The treatment was successful because a complete regression of the effusion after one month was evidenced, with a substantial modification towards normality of the thyroid function tests. One year later, the substitutive therapy led to complete normalization of the thyroid function indexes. A slight reduction of weight (BMI, 17.60 for age) and an increase of the velocity of height growth were evidenced. Conclusions: When fluid is identified in the pericardial space and pericarditis of unknown origin is diagnosed, the thyroid function should be immediately evaluated to prescribe substitutive hormonal therapy if necessary and thereby avoid overt hypothyroidism development and the risk of cardiac tamponade.
Prevalence of Chlamydia trachomatis Infection and Its Association with Sexual Behaviour and Alcohol Use in the Population Living in Separated and Segregated Roma Settlements in Eastern Slovakia
Authors: Babinská Ingrid , Halánová Monika , Kalinová Zuzana , Čechová Lenka , Čisláková Lýdia , Madarasová Gecková Andrea
Published: December 2017

The aim of the study was to explore sexual behaviour and the occurrence of Chlamydia trachomatis (CT) infection in the population living in Roma settlements compared to the majority population in Slovakia and to assess the association between alcohol use and sexual behaviour within both populations. A cross-sectional population-based Hepa-Meta study was conducted in Slovakia in 2011. The final sample comprised 452 Roma and 403 non-Roma respondents. The occurrence of CT was detected by direct proof of the pathogen by PCR. The association between alcohol use and the prevalence of risky sexual behaviour were assessed using a logistic regression. First intercourse at age 15 or younger was reported by 27.9% of Roma (vs. 4.5% of non-Roma); 93.4% of Roma (vs. 77.9% of non-Roma) used condom inconsistently, 22.8% of Roma (vs. 43.9% of non-Roma) used a condom for protection from unwanted pregnancies and only 8.8% of Roma (vs. 21.8% of non-Roma) due to protection against infectious diseases. However, Roma reported having had five or more sexual partners less often compared to the majority (11.5% of Roma vs. 20.6% of non-Roma). Binge drinking at least once a month was associated with a higher number of sexual partners in both groups, but not with condom non-use. The prevalence of CT infection in the Roma population was higher (3.8%) compared to non-Roma (2.7%); however, the difference was not statistically significant. Our study found no differences in the prevalence of CT infection between Roma and non-Roma despite differences in sexual behaviour. Roma begin their sexual life earlier and have unprotected sex more often, but on the other hand, they seem to be much more restrained in terms of the number of sexual partners compared to the majority population.
Essential Oils from Neotropical Piper Species and Their Biological Activities
Authors: Da Silva Joyce , Da Trindade Rafaela , Alves Nayara , Figueiredo Pablo , Maia José , Setzer William
Published: December 2017

The Piper genus is the most representative of the Piperaceae reaching around 2000 species distributed in the pantropical region. In the Neotropics, its species are represented by herbs, shrubs, and lianas, which are used in traditional medicine to prepare teas and infusions. Its essential oils (EOs) present high yield and are chemically constituted by complex mixtures or the predominance of main volatile constituents. The chemical composition of Piper EOs displays interspecific or intraspecific variations, according to the site of collection or seasonality. The main volatile compounds identified in Piper EOs are monoterpenes hydrocarbons, oxygenated monoterpenoids, sesquiterpene hydrocarbons, oxygenated sesquiterpenoids and large amounts of phenylpropanoids. In this review, we are reporting the biological potential of Piper EOs from the Neotropical region. There are many reports of Piper EOs as antimicrobial agents (fungi and bacteria), antiprotozoal (Leishmania spp., Plasmodium spp., and Trypanosoma spp.), acetylcholinesterase inhibitor, antinociceptive, anti-inflammatory and cytotoxic activity against different tumor cells lines (breast, leukemia, melanoma, gastric, among others). These studies can contribute to the rational and economic exploration of Piper species, once they have been identified as potent natural and alternative sources to treat human diseases.
Clodronate as a Therapeutic Strategy against Osteoarthritis
Authors: Valenti Maria , Mottes Monica , Biotti Alessandro , Perduca Massimiliano , Pisani Arianna , Bovi Michele , Deiana Michela , Cheri Samuele , Dalle Carbonare Luca
Published: December 2017

Osteoarthritis (OA), the most prevalent musculoskeletal pathology, is mainly characterized by the progressive degradation of articular cartilage due to an imbalance between anabolic and catabolic processes. Consequently, OA has been associated with defects in the chondrocitic differentiation of progenitor stem cells (PSCs). In addition, SOX9 is the transcription factor responsible for PSCs chondrogenic commitment. To evaluate the effects of the non-amino bisphosphonate clodronate in OA patients we investigated SOX9 gene expression in circulating progenitor cells (CPCs) and in an in vitro OA model. We evaluated pain intensity, mental and physical performance in OA patients, as well as serum biomarkers related to bone metabolism. In addition, in order to improve therapeutic strategies, we assayed nanoparticle-embedded clodronate (NPs-clo) in an in vitro model of chondrogenic differentiation. Our data showed upregulation of SOX9 gene expression upon treatment, suggesting an increase in chondrocytic commitment. Clodronate also reduced osteoarticular pain and improved mental and physical performance in patients. Furthermore, NPs-clo stimulated SOX9 expression more efficaciously than clodronate alone. Clodronate may therefore be considered a good therapeutic tool against OA; its formulation in nanoparticles may represent a promising challenge to counteract cartilage degeneration.
The Toxicity of Nanoparticles Depends on Multiple Molecular and Physicochemical Mechanisms
Authors: Huang Yue-Wern , Cambre Melissa , Lee Han-Jung
Published: December 2017

Nanotechnology is an emerging discipline that studies matters at the nanoscale level. Eventually, the goal is to manipulate matters at the atomic level to serve mankind. One growing area in nanotechnology is biomedical applications, which involve disease management and the discovery of basic biological principles. In this review, we discuss characteristics of nanomaterials, with an emphasis on transition metal oxide nanoparticles that influence cytotoxicity. Identification of those properties may lead to the design of more efficient and safer nanosized products for various industrial purposes and provide guidance for assessment of human and environmental health risk. We then investigate biochemical and molecular mechanisms of cytotoxicity that include oxidative stress-induced cellular events and alteration of the pathways pertaining to intracellular calcium homeostasis. All the stresses lead to cell injuries and death. Furthermore, as exposure to nanoparticles results in deregulation of the cell cycle (i.e., interfering with cell proliferation), the change in cell number is a function of cell killing and the suppression of cell proliferation. Collectively, the review article provides insights into the complexity of nanotoxicology.
Heat Shock Proteins in Vascular Diabetic Complications: Review and Future Perspective
Authors: Bellini Stefania , Barutta Federica , Mastrocola Raffaella , Imperatore Luigi , Bruno Graziella , Gruden Gabriella
Published: December 2017

Heat shock proteins (HSPs) are a large family of proteins highly conserved throughout evolution because of their unique cytoprotective properties. Besides assisting protein refolding and regulating proteostasis under stressful conditions, HSPs also play an important role in protecting cells from oxidative stress, inflammation, and apoptosis. Therefore, HSPs are crucial in counteracting the deleterious effects of hyperglycemia in target organs of diabetes vascular complications. Changes in HSP expression have been demonstrated in diabetic complications and functionally related to hyperglycemia-induced cell injury. Moreover, associations between diabetic complications and altered circulating levels of both HSPs and anti-HSPs have been shown in clinical studies. HSPs thus represent an exciting therapeutic opportunity and might also be valuable as clinical biomarkers. However, this field of research is still in its infancy and further studies in both experimental diabetes and humans are required to gain a full understanding of HSP relevance. In this review, we summarize current knowledge and discuss future perspective.
Cost and Cost-Effectiveness Assessments of Newborn Screening for Critical Congenital Heart Disease Using Pulse Oximetry: A Review
Authors: Grosse Scott , Peterson Cora , Abouk Rahi , Glidewell Jill , Oster Matthew
Published: December 2017

Screening newborns for critical congenital heart disease (CCHD) using pulse oximetry is recommended to allow for the prompt diagnosis and prevention of life-threatening crises. The present review summarizes and critiques six previously published estimates of the costs or cost-effectiveness of CCHD screening from the United Kingdom, United States, and China. Several elements that affect CCHD screening costs were assessed in varying numbers of studies, including screening staff time, instrumentation, and consumables, as well as costs of diagnosis and treatment. A previous US study that used conservative assumptions suggested that CCHD screening is likely to be considered cost-effective from the healthcare sector perspective. Newly available estimates of avoided infant CCHD deaths in several US states that implemented mandatory CCHD screening policies during 2011–2013 suggest a substantially larger reduction in deaths than was projected in the previous US cost-effectiveness analysis. Taking into account these new estimates, we estimate that cost per life-year gained could be as low as USD 12,000. However, that estimate does not take into account future costs of health care and education for surviving children with CCHD nor the costs incurred by health departments to support and monitor CCHD screening policies and programs.
Cognitive and Motivational Monitoring during Enriched Sport Activities in a Sample of Children Living in Europe. The Esa Program
Authors: Alesi Marianna , Silva Carlos , Borrego Carla , Monteiro Diogo , Genchi Rosario , Polizzi Valentina , Kirkar Musa , Demetriou Yolanda , Brame Judith , Şahin Fatma , Kızılyallı Meltem , Gómez-López Manuel , López Sánchez Guillermo , Pajaujiene Simona , Indriuniene Vinga , Rađa Ante , Bianco Antonino
Published: December 2017

Enriched Sport Activities (ESA) Program is an Evidence-based Practice Exercise Program cofounded by the Erasmus + Programme of the European Union (Key action: Sport-579661-EPP-1-2016-2-IT-SPO-SCP) [...]
Deep Learning vs. Conventional Machine Learning: Pilot Study of WMH Segmentation in Brain MRI with Absence or Mild Vascular Pathology
Authors: Rachmadi Muhammad , Valdés-Hernández Maria , Agan Maria , Komura Taku
Published: December 2017

In the wake of the use of deep learning algorithms in medical image analysis, we compared performance of deep learning algorithms, namely the deep Boltzmann machine (DBM), convolutional encoder network (CEN) and patch-wise convolutional neural network (patch-CNN), with two conventional machine learning schemes: Support vector machine (SVM) and random forest (RF), for white matter hyperintensities (WMH) segmentation on brain MRI with mild or no vascular pathology. We also compared all these approaches with a method in the Lesion Segmentation Tool public toolbox named lesion growth algorithm (LGA). We used a dataset comprised of 60 MRI data from 20 subjects in the Alzheimer’s Disease Neuroimaging Initiative (ADNI) database, each scanned once every year during three consecutive years. Spatial agreement score, receiver operating characteristic and precision-recall performance curves, volume disagreement score, agreement with intra-/inter-observer reliability measurements and visual evaluation were used to find the best configuration of each learning algorithm for WMH segmentation. By using optimum threshold values for the probabilistic output from each algorithm to produce binary masks of WMH, we found that SVM and RF produced good results for medium to very large WMH burden but deep learning algorithms performed generally better than conventional ones in most evaluations.
A Comparison of the Grammatical Production of Child Heritage Speakers of Spanish across Language and Grade: Kindergarten and Grade 1
Authors: Rodriguez Estrella , Bustamante Kristina , Wood Carla , Sunderman Gretchen
Published: December 2017

In this study, we elicited grammatical forms (oral production) from a group of child heritage speakers of Spanish (N = 45) in English and Spanish, using the morphosyntax subtest of the Bilingual English–Spanish Assessment (BESA), (Peña et al. 2014). A cross-sectional design was used with 25 participants in kindergarten and 20 in first grade. All children spoke Spanish at home and attended English rural schools. We controlled for L2 class environment and socio-economic status. Research findings indicated children produced more target structures in L1 Spanish. This project supports the view that sequential bilingualism and continuous exposure to the heritage language may assist heritage speakers to maintain some L1 structures (Miller and Cuza 2013; Pascual y Cabo and Gómez Soler 2015). Patterns of L2 development are also addressed.
A New Protection Orientation and Framework for Refugees and Other Forced Migrants
Authors: Simeon James
Published: December 2017

The unprecedented current “refugee crisis,” with its 65 million plus uprooted people, demands a new protection orientation and framework for refugees and other forced migrants that are focused on the “root causes” of refugeehood, non-international protracted armed conflict or civil war. It is argued that four essential reforms are required to the international refugee protection system to respond to the “root causes” of refugees in the world today. The first calls for broadening the definition of who is a refugee to include “war refugees” as found in the 1984 Cartagena Declaration and the 1969 Organization of African Unity (OAU) Convention. The 1984 Cartagena Declaration is preferred because it has the most progressive and broadest legal definition of who is a refugee and, therefore, should be emulated by all regions and the UNHCR internationally. The second reform would be the adoption of the Cartagena Declaration decennial consultation process and its comprehensive Plan of Action by the UNHCR on a global basis. This process has proven to be a success in Latin America and ought to be adopted internationally to develop and to realize the progressive advancement of international protection for all persons who are fleeing persecution and armed conflict. The third major reform is to develop the capacity of the UN to be able to operate in the midst of an armed conflict situation in order to broker a ceasefire and, then, negotiate a peace agreement, particularly, in those situations where massed forced displacement has taken place or potentially could take place. The fourth reform calls for the UN to expand the Responsibility to Protect (R2P) doctrine to incorporate massed forced displacement, in addition to, war crimes, crimes against humanity, ethnic cleansing, and, genocide. This further implies that mass forced displacement ought to be criminalized and made a serious international crime, not simply in the international humanitarian law and international criminal law sense of forced deportation, transfers, and expulsions, by opposing military forces, but in situations of armed conflict when people flee of their own volition in order to save their lives. These four reforms do not require a reformulation or reconceptualization of the international refugee protection system but a reform of a number of key elements that would simultaneously address the “root causes” of refugees and, especially, mass forced displacement that is due principally to non-international protracted armed conflict or seemingly endless civil wars.
The Potential of Frog Skin-Derived Peptides for Development into Therapeutically-Valuable Immunomodulatory Agents
Authors: Pantic Jelena , Jovanovic Ivan , Radosavljevic Gordana , Arsenijevic Nebojsa , Conlon J. , Lukic Miodrag
Published: December 2017

The aim of this article is to review the immunoregulatory actions of frog skin-derived peptides in order to assess their potential as candidates for immunomodulatory or anti-inflammatory therapy. Frog skin peptides with demonstrable immunomodulatory properties have been isolated from skin secretions of a range of species belonging to the families Alytidae, Ascaphidae, Discoglossidae, Leptodactylidae, Pipidae and Ranidae. Their effects upon production of inflammatory and immunoregulatory cytokines by target cells have been evaluated ex vivo and effects upon cytokine expression and immune cell activity have been studied in vivo by flow cytometry after injection into mice. The naturally-occurring peptides and/or their synthetic analogues show complex and variable actions on the production of proinflammatory (TNF-α, IL-1β, IL-12, IL-23, IL-8, IFN-γ and IL-17), pleiotropic (IL-4 and IL-6) and immunosuppressive (IL-10 and TGF-β) cytokines by peripheral and spleen cells, peritoneal cells and/or isolated macrophages. The effects of frenatin 2.1S include enhancement of the activation state and homing capacity of Th1-type lymphocytes and NK cells in the mouse peritoneal cavity, as well as the promotion of their tumoricidal capacities. Overall, the diverse effects of frog skin-derived peptides on the immune system indicate their potential for development into therapeutic agents.
Efficient Enzymatic Routes for the Synthesis of New Eight-membered Cyclic β-Amino Acid and β-Lactam Enantiomers
Authors: Forró Enikő , Kiss Loránd , Árva Judit , Fülöp Ferenc
Published: December 2017

Efficient enzymatic resolutions are reported for the preparation of new eight-membered ring-fused enantiomeric β-amino acids [(1R,2S)-9 and (1S,2R)-9] and β-lactams [(1S,8R)-3, (1R,8S)-3 (1S,8R)-4 and (1R,8S)-7], through asymmetric acylation of (±)-4 (E > 100) or enantioselective hydrolysis (E > 200) of the corresponding inactivated (±)-3 or activated (±)-4 β-lactams, catalyzed by PSIM or CAL-B in an organic solvent. CAL-B-catalyzed ring cleavage of (±)-6 (E > 200) resulted in the unreacted (1S,8R)-6, potential intermediate for the synthesis of enantiomeric anatoxin-a. The best strategies, in view of E, reaction rate and product yields, which underline the importance of substrate engineering, are highlighted.
Nucleobase–Guanidiniocarbonyl-Pyrrole Conjugates as Novel Fluorimetric Sensors for Single Stranded RNA
Authors: Ban Željka , Žinić Biserka , Vianello Robert , Schmuck Carsten , Piantanida Ivo
Published: December 2017

We demonstrate here for the first time that a guanidiniocarbonyl-pyrrole (GCP) unit can be applied for the fine recognition of single stranded RNA sequences—an intuitively unexpected result since so far binding of the GCP unit to ds-DNA or ds-RNA relied strongly on minor or major groove interactions, as shown in previous work. Two novel nucleobase–GCP isosteric conjugates differing in the flexibility of GCP unit revealed a fluorimetric recognition of various single stranded RNA, which could be additionally regulated by pH. The more rigid conjugate showed a specific fluorescence increase for poly A only at pH 7, whereby this response could be reversibly switched-off at pH 5. The more flexible derivative revealed selective fluorescence quenching by poly G at pH 7 but no change for poly A, whereas its recognition of poly AH+ can be switched-on at pH 5. The computational analysis confirmed the important role of the GCP fragment and its protonation states in the sensing of polynucleotides and revealed that it is affected by the intrinsic dynamical features of conjugates themselves. Both conjugates showed a negligible response to uracil and cytosine ss-RNA as well as ds-RNA at pH 7, and only weak interactions with ds-DNA. Thus, nucleobase–GCP conjugates can be considered as novel lead compounds for the design of ss-RNA or ss-DNA selective fluorimetric probes.
Sequential Combination of Microwave- and Ultrasound-Assisted Extraction of Total Flavonoids from Osmanthus fragrans Lour. Flowers
Authors: Yu Jianfeng , Lou Qi , Zheng Xiangyang , Cui Zhengwei , Fu Jian
Published: December 2017

Microwave-assisted and ultrasound-assisted extraction assays were used to isolate total flavonoids (TF) from Osmanthus fragrans flowers. The effects of the solid-liquid ratio, ethanol concentration, microwave power, microwave extraction time, ultrasonic power and ultrasonic extraction time on the yield of TF were studied. A sequential combination of microwave- and ultrasound-assisted extraction (SC-MUAE) methods was developed, which was subsequently optimized by Box-Behnken design-response surface methodology (BBD-RSM). The interaction effects of the ethanol concentration (40–60%), microwave extraction time (5–7 min), ultrasonic extraction time (8–12 min) and ultrasonic power (210–430 W) on the yield of TF were investigated. The optimum operating parameters for the extraction of TF were determined to be as follows: ethanol concentration (48.15%), microwave extraction time (6.43 min), ultrasonic extraction time (10.09 min) and ultrasonic power (370.9 W). Under these conditions, the extraction yield of TF was 7.86 mg/g.
Evaluation on the Photosensitivity of 2,2′-Azobis(2,4-Dimethyl)Valeronitrile with UV
Authors: Yang Yi , Tsai Yun-Ting
Published: December 2017

Azo compounds have high exothermic characteristics and low thermal stability, which have caused many serious thermal accidents around the world. In general, different locations (e.g., equatorial or polar regions) have different UV intensities. If the azo compound exists in an inappropriately stored or transported condition, the decrease in thermal stability may cause a thermal hazard or ageing. 2,2′-Azobis(2,4-dimethyl)valeronitrile (ADVN) is investigated with respect to the thermal stability affected by UV exposure at 0, 6, 12, and 24 h. When ADVN is exposed to 24 h of UV (100 mW/m2 and 254 nm), T0 is not only advanced, but the mass loss is also increased during the main decomposition stage. In addition, the apparent activation energy and integral procedural decomposition temperature (IPDT) of ADVN exposed to 24 h of UV is calculated by kinetic models. Therefore, the prevention mechanism, thermal characteristics, and kinetic parameters are established in our study. We should isolate UV contacting ADVN under any situations, avoiding ADVN being aged or leading to thermal runaway. This study provided significant information for a safer process under changing UV exposure times for ADVN. Furthermore, the research method may serve as an important benchmark for handling potentially hazardous chemicals, such as azo compounds described herein.
Construction of Luminogen Exhibiting Multicolored Emission Switching through Combination of Twisted Conjugation Core and Donor-Acceptor Units
Authors: Tian Haiyan , Tang Xi , Dong Yong
Published: December 2017

Stimuli responsive luminescent materials, especially those exhibiting multicolor emission switching, have potential application in sensor, optical recording, security ink, and anti-counterfeit label. Through combination of twisted conjugation core and donor and acceptor units, a luminogen (2-(bis(4-(carbazol-9-yl)phenyl)methylene)malononitrile (1) was synthesized. Luminogen 1 can form three kinds of crystals emitting green (1GC, λem = 506 nm, ΦF = 19.8%), yellow-green (1YC, λem = 537 nm, ΦF = 17.8%), and orange (1OC, λem = 585 nm, ΦF = 30.0%) light upon 365 nm UV illumination. The emission of amorphous solid of 1 (1Am) overlaps with that of 1OC (λem = 585 nm), with quantum yield of 13.9%, which is seldom reported. Emission of 1 can be switched among green, yellow-green, and orange through morphology modulation upon exposure to thermal, solvent vapor, or mechanical stimuli. Finally, its potential application in optical recording was also investigated.
Multi-Objective Optimization Algorithm to Discover Condition-Specific Modules in Multiple Networks
Authors: Ma Xiaoke , Sun Penggang , Zhao Jianbang
Published: December 2017

The advances in biological technologies make it possible to generate data for multiple conditions simultaneously. Discovering the condition-specific modules in multiple networks has great merit in understanding the underlying molecular mechanisms of cells. The available algorithms transform the multiple networks into a single objective optimization problem, which is criticized for its low accuracy. To address this issue, a multi-objective genetic algorithm for condition-specific modules in multiple networks (MOGA-CSM) is developed to discover the condition-specific modules. By using the artificial networks, we demonstrate that the MOGA-CSM outperforms state-of-the-art methods in terms of accuracy. Furthermore, MOGA-CSM discovers stage-specific modules in breast cancer networks based on The Cancer Genome Atlas (TCGA) data, and these modules serve as biomarkers to predict stages of breast cancer. The proposed model and algorithm provide an effective way to analyze multiple networks.