bluuebunny/crossref_metadata_2025 huggingface dataset:
El derecho al recurso: lectura constitucional a propósito del sistema recursivo en el procedimiento arbitral chileno
Authors: Vásquez Palma M. Fernanda , Delgado Castro Jordi
Published: December 2019

El presente trabajo aborda una problemática muy contingente en nuestro ordenamiento jurídico: el derecho al recurso y su impacto en procedimientos civiles y comerciales, a tenor de qué ha de entenderse como un proceso que se caracterice por otorgar una genuina tutela judicial efectiva. Para ello, la principal tesis que se sostiene es que la Constitución Política de Chile consagra, específicamente, en concordancia con los tratados internacionales aplicables a nuestro país, un derecho al recurso que contemple una revisión amplia al modo que se vislumbra para el recurso de apelación. En el presente artículo consideramos que esa tesis no puede extrapolarse al procedimiento arbitral, pues el sistema recursivo de aquel es restrictivo, para destacar, entonces, que la jurisdicción estatal contempla una forma alternativa de enjuiciamiento y, en especial, de control y revisión de los fallos de primera instancia. Este fenómeno resultaría compatible con nuestro sistema constitucional y con el Sistema Interamericano de Derechos Humanos.
Solución de conflictos de competencias municipales: Ecuador
Authors: Torres Maldonado Ángel Eduardo
Published: December 2019

El modelo clásico de centralización política busca ser superado a partir de la reforma democrática del Estado unitario descentralizado que prescribe la Constitución ecuatoriana de 2008; sin embargo, su eficacia enfrenta dificultades que se deben, entre otras razones, a la interpretación restrictiva de la facultad legislativa atribuida a los niveles de gobierno local, en materias de su competencia exclusiva. El objeto de este estudio es discutir en qué medida puede ser eficaz el principio de competencia para fortalecer el gobierno descentralizado y revisar los límites impuestos a la actividad legislativa de la Asamblea Nacional sobre materias de competencia exclusiva de los gobiernos autónomos descentralizados. La tesis propuesta consiste en afirmar la prevalencia de los actos normativos de los niveles de gobierno local que regulan el ejercicio de competencias exclusivas sobre disposiciones normativas de rango legal, en virtud del principio de competencia. Para argumentar este planteamiento, la primera parte contiene reflexiones teórico descriptivas sobre conceptos fundamentales relativos a los alcances del principio de gobierno unitario descentralizado; la segunda parte, abarca un análisis empírico de reflexión crítica respecto a sentencias de control de legalidad y constitucionalidad; y, al final, consta una breve reflexión inherente a los principios que son aplicables a la coherencia entre ordenanzas, leyes y la Constitución ecuatoriana.
L’hybridité générique dans Syngué Sabour. Pierre de patience d’Atiq Rahimi
Authors: Körömi Gabriella
Published: December 2019

Appartenir à deux cultures, deux civilisations, deux langues est une source de richesse dans laquelle puise Atiq Rahimi, écrivain et cinéaste franco-afghan. Il n’y a rien de surprenant à ce que toute son oeuvre soit construite sur l’hybridité identitaire, culturelle, générique ou dialogique. L’analyse de son premier roman écrit en français, Syngué Sabour. Pierre de patience (Prix Goncourt 2008), démontre que le ressort du texte est l’hybridité générique. La présente étude a pour objectif d’analyser, d’une part, la coexistence des différents arts (théâtre, cinéma, musique) dans le roman, et d’autre part, d’examiner par quels moyens et pour quelles raisons le romancier allie le roman, genre dominant de l’Occident, avec le conte, genre traditionnel oral de l’Orient, et ce que le dialogue de ces deux genres peut apporter à la littérature française.
Polemiche a distanza fra Firenze e Venezia. Sulla perduta «Risposta» di Paolo Paruta alla «Lettera XXX» dello pseudo-Dante a Guido da Polenta (secondo XVI sec.)
Authors: Giani Marco
Published: December 2019

Il saggio presenta tutti i dati disponibili utili al ritrovamento di un testo per ora fantasma, quale la Risposta di Paolo Paruta (Venezia, 1540 - 1598) alla Lettera XXX dello pseudo-Dante, falso d’autore editando il quale nel 1547 il fiorentino Anton Francesco Doni scatenò una violenta polemica politica anti-veneziana. Nuove ricerche dimostrano come nella celebre biblioteca padovana di Gian Vincenzo Pinelli fosse conservata una copia della Risposta. Paruta e Pinelli, infatti, condividevano molti interessi e conoscenze negli ambienti della Padova e della Venezia del Secondo Cinquecento.
La conceptualización lingüística del tabú en el discurso humorístico subversivo
Authors: Linares Bernabéu Esther
Published: December 2019

En la presente investigación, analizamos el papel del tabú como un elemento clave en el discurso humorístico subversivo para la transgresión de lo establecido (Mintz, 1985). En este sentido, partimos de la hipótesis general de que las cómicas analizadas conceptualizan tabúes relacionados con su identidad de género, por medio de eufemismos, disfemismos y ortofemismos, para romper con ciertos roles asociados a la mujer. Además, como segunda hipótesis de partida, creemos que el porcentaje de temas tabú está estrechamente relacionado con el estilo humorístico individual de cada cómica y que, por tanto, nos encontraremos con cifras muy dispares. Así pues, el presente trabajo examina la expresión del tabú por medio de la comunicación ortofemística, disfemística y eufemística en un corpus de análisis que recoge 15 monólogos segmentados en 504 secuencias humorísticas y que han sido representados por 15 monologuistas españolas. Los resultados de este estudio confirman que la comedia en vivo aporta el espacio público y social ideal para transgredir tabúes y, en el caso de la comedia femenina subversiva, sirve también para romper con algunos estigmas relacionados con la identidad de género.
Distribusi Spasial Logam Berat Besi (Fe) di Perairan Teluk Staring, Sulawesi Tenggara
Authors: Bahril Bahril , Armid Armid , Jabir Jabir , Takwir Amadhan , Rahim Abdul
Published: December 2019

 

Study on spatial distribution of metal Fe in the coastal area of Staring Bay, Southeast Sulawesi has been carried out. This study aims to determine the spatial distributions of heavy Fe in the coastal area of Staring Bay. Sampling was conducted at 12 stations along the bay through the purposive sampling method. Determination of heavy metal Fe concentration was performed utilizing the Atomic Absorption Spectrophotometry (AAS). Spatial analysis was performed with IDW interpolation method using a software ArcGIS 10.3. The results showed that the concentration of metal Fe at 12 stations was in the range of 0.00187 ppm to 0.01296 ppm whereby the highest level was found at station 7 (mouth of the Laonti river). The spatial analysis in 12 stations confirmed that in the coastal areas of Woru-woru village (near the mouth of the Laonti river) to Gala Island and Wandahea village up to Intan Island have been contaminated by metal Fe ranged from 0.0106-0.0117 ppm and 0.0118-0.013 ppm, respectively. The contamination factor of metal Fe was moderate. Based on the values of CF, the highest contamination of heavy metal Fe is at station 7.

 

Keywords: Spatial analysis, Fe, IDW, CF, Staring Bay

 

 

Telah dilakukan studi distribusi spasial logam Fe di perairan Teluk Staring, Sulawesi Tenggara. Penelitian ini bertujuan untuk menentukan sebaran spasial logam Fe di perairan Teluk Staring. Pengambilan sampel dilakukan 12 stasiun di perairan teluk dengan metode purposive sampling. Penentuan kadar logam Fe pada sampel air laut menggunakan Atomic Absorption Spectrophotometry (AAS). Analisis spasial dilakukan dengan metode interpolasi IDW menggunakan software ArcGIS 10.3. Hasil penelitian menunjukkan bahwa kadar logam Fe di 12 stasiun penelitian berkisar antara 0,00187-0,01296 ppm dengan kadar tertinggi berada pada stasiun 7 (muara Sungai Laonti). Hasil analisis spasial pada 12 stasiun menunjukkan bahwa di daerah pesisir Desa Woru-woru (dekat muara Sungai Laonti) hingga Pulau Gala dan Desa Wandahea hingga Pulau Intan telah terkontaminasi logam Fe dengan kisaran 0,0106-0,0117 ppm dan 0,0118-0,013 ppm. Faktor kontaminasi logam Fe bersifat moderat. Berdasarkan nilai CF, kontaminasi tertinggi oleh logam Fe terdapat pada stasiun 7.

 

Kata kunci: Analisis spasial, Fe, IDW, CF, Teluk Staring

 
LOGIKA MIHAJLA MARKOVIĆA KAO FILOZOFSKA PROPEDEUTIKA U SREDNJOJ ŠKOLI
Authors: Cmiljanović Anja S.
Published: December 2019

Autorka u radu ispituje mogućnost uloge logike kao filozofske propedeutike u srednjoškolskom sistemu obrazovanja. Ukoliko se logika shvata kao nauka koja se bavi osnovnim elementima i principima mišljenja, onda je razumljivo zašto se pre pristupa izučavanju predmeta filozofije u završnoj godini srednje škole mora pažljivo pristupiti izučavanju predmeta nauke logike. Stoga ćemo se u radu najpre posvetiti istraživanju mogućnosti da se logika razume kao filozofska propedeutika kako kroz preispitivanje istorijske pozicije logike tako i kroz problematizaciju samog pojma filozofije. Nakon toga, daćemo kratak prikaz udžbenika logike Mihajla Markovića za gimanziju i treći razred pravno-birotehničke škole, kao osnovnog nastavnog sredstva u procesu nastave Logike, da bismo videli na koji način se sadržaj logike može približiti učenicima srednjih škola, kao i da li je Mihajlovićev udžbenik adekvatan za takav cilj. Naposletku, ispitaćemo važnost uloge koju logika ima u razvoju kritičkog mišljenja i način na koji može da doprinese tom razvoju, te tematizovati najbolje nastavne metode kojim se može pristupiti nastavi Logike u tu svrhu.
KONSTRUKCIJA ZNANJA I INTERAKCIJA NA UNIVERZITETSKIM PREDAVANJIMA DIGITALNOG DOBA
Authors: Zobenica Nikolina N.
Published: December 2019

Predavanja su obavezni deo univerzitetskog obrazovanja u Srbiji. Propisuje se, čak, 50 do 60% predavanja u odnosu na druge oblike nastave kao što su vežbe i seminari. Na taj način se stiče utisak da se predavanjima pridaje veliki značaj, iako se ona već odavno smatraju zastarelim i neefikasnim vidom nastave, budući da im je uglavnom svrha samo prenošenje znanja, bez aktiviranja viših kognitivnih sposobnosti kod studenata[1]. Kritika uglavnom stiže iz pravca konstruktivizma i komunikativne didaktike, što predstavlja podsticaj za reviziju ovog oblika nastave, kao i za primenu digitalnih medija u cilju inovacije i modernizacije univerzitetskih predavanja. Cilj ovog rada je da se izlože odlike predavanja, njihova kritika i predlozi prevazilaženja njihovih slabosti korišćenjem digitalnih medija koji pružaju mogućnost za samostalnu konstrukciju znanja i interakciju studenata s nastavnim materijalima.

[1] Svi pojmovi u ovom radu koji su upotrebljeni u muškom gramatičkom rodu obuhvataju muški i ženski rod lica na koja se odnose.
МОРФОЛОШКО-СИНТАКСИЧКИ МИНИМАЛНИ РЕЧНИК ГЛАГОЛА У НАСТАВИ СРПСКОГ ЈЕЗИКА КАО СТРАНОГ / ДРУГОГ И КАО МАТЕРЊЕГ
Authors: Бабић Биљана М.
Published: December 2019

У раду се: 1) презентује морфолошко-синтаксички минимални речник глагола, како би се показали принципи његовог уређења и његов садржај, 2) анализира грађење презента и императива у српском језику према релевантним граматикама, како би се указало на проблеме у вези с овим облицима који потичу од неусклађености приступâ и мишљењâ у тим граматикама, и препоручује најједноставнији приступ у обради ова два глаголска облика, 3) дају чести примери нарушене норме у употреби глаголских облика, а од стране изворних говорника, како би се показало да за оваквим речником постоји и шира потреба него што је настава српског као страног / другог.
Narrativas e excesso: relações das imagens ubíquas
Authors: Valandro Charmeni Vargas
Published: December 2019

Esta pesquisa propõem ressignificar os arquivos pessoais encontrados na memória de um aparelho celular correspondente ao período de um ano (2017) através da criação de uma videoinstalação. O texto traz questões referentes a imagens, excesso, narrativas e ubiquidade, sob o viés dos autores pesquisados no referencial teórico, que são, entre outros, Vilém Flusser, Diana Domingues, Julio Plaza, Priscila Arantes e Christine Mello. São abordados também o processo de criação da obra e a interseção entre as linguagens midiáticas e a arte digital. A criação da poética, desta forma se mostra mais na elaboração e desenvolvimento da pesquisa-obra, do que no objeto acabado. Pois, como vídeoinstalção que se instaura no espaço expositivo a posteriori, em relação à escrita deste texto, certamente ocorrerão percepções que não poderão estar presentes no espaço de reflexão que dá corpo à este artigo.
Expediente Revista nº 39
Authors: Dal Bello Márcia Pessoa
Published: December 2019

Equipe e ficha técnica
A paleontologia na perspectiva do ensino
Authors: Hohemberger Rômulo , Schwanke Cibele , Bilar Jéssica de Góes , Coutinho Renato Xavier
Published: December 2019

O ensino de paleontologia pressupõe compreender o processo evolutivo, baseado em evidências: os fósseis. Logo, analisar a produção cientifica sobre este tema é relevante, pois através desta, podemos verificar de forma indireta se a abordagem, além de desenvolvida em sala de aula, está sendo publicada. Neste sentido, procurou-se identificar a produção sobre o tema através de uma análise cienciométrica, baseada em 3 plataformas de busca: Plataforma Sucupira, Periódicos Capes e Google Acadêmico. Nos espaços de busca utilizou-se o termo Paleontologia e o período para análise é de 2013-2017. Nesse sentido, encontrou-se um total 125 resultados, após, como a proposta se baseia em analisar apenas a produção sobre o ensino de Paleontologia, chegou-se ao total de 22 artigos. Analisando estes, 11 eram descritos de maneira teórica e 11 artigos de maneira aplicada. Nesse sentido, observa-se a pouca publicação na área e a inexistência de um periódicos que publique sobre o tema.
Konsep Islamic corporate governance sebagai tata kelola perusahaan Bakpiapia Djogja
Authors: Hasanah Shofia Mauizotun , Kurniawan Romi
Published: December 2019

Purpose — This study tries to build a paradigm and implementation of Islamic corporate governance in a company.Research method — This research uses qualitative research methods and descriptive data. The sample in this study is Bakpiapia Djogja company. The data used is a report on the implementation of Islamic corporate governance.Result — The study found that Bakpiapia Djogja company has implemented a small part of the principles contained in the concept of Islamic Corporate Governance.
Sikap masyarakat terhadap pengembangan Pantai Loang Baloq sebagai daya tarik wisata di Kota Mataram
Authors: Nugroho M. Setyo
Published: December 2019

Purpose — This study aims to determine the community's attitude towards the development of Loang Baloq Beach in Mataram City.Research method — Data were collected using observation techniques to Loang Baloq Beach, documentation studies in the form of documents related to Loang Baloq Beach, and distributing questionnaires to 100 people (accidental sampling) around Loang Baloq Beach, Mataram City.Result — The result showed that the community agrees with the development of Loang Baloq Beach in the hope that it can become an economic locomotive and a place to promote Loang Baloq Beach at the international level. However, there are still some people who do not agree with the development of Loang Baloq Beach. This is because they think that tourism brings more harm than benefits.
Práticas Desenvolvidas por Psicólogos em Serviços de Atenção Psicossocial: Revisão de Literatura
Authors: Assenheimer Stephannie , Pegoraro Renata Fabiana
Published: December 2019

Este artigo tem por objetivo geral mapear as práticas dos psicólogos nos Centros de Atenção Psicossocial (CAPS) por meio de uma revisão integrativa de literatura publicada entre 2005 a 2014, em periódicos indexados nas bases de dados on-line Scielo, Biblioteca Virtual em Saúde e Redalyc, a partir das palavras-chave: Psicologia, Psicólogo, Centros de Atenção Psicossocial. A amostra foi composta por 12 artigos publicados de 2005 a 2012, cujas respostas à pergunta norteadora foram agrupadas em função das temáticas abordadas: (1) Atividades desenvolvidas pelo psicólogo nos CAPS; (2) A formação do psicólogo para o trabalho no CAPS e a necessidade de capacitação profissional; (3) Concluímos que é urgente o investimento em Educação Permanente e dos Cursos de Psicologia revejam o ensino das disciplinas sobre saúde pública.
İNGİLİZ KRALİYET AFRİKAN ŞİRKETİ 1672-1752: KALELERİ, KÖLE VE ALTIN TİCARETİ
Authors: GÜNARSLAN Hüseyin
Published: December 2019

Afrika ve onun insanları sömürge tarihi boyunca en
fazla istismar edilenler arasındadır. Ülkenin kaynaklarının yanı sıra insanları
da alınıp satılmıştır. Batı medeniyeti bu kıtada etkinlik kurabilmek için büyük
çaba harcamıştır. İngiliz Kraliyet Afrikan Şirketi´de kurulduğu ilk günden
itibaren bu kıtanın zenginliklerinin peşine düşmüştür. Atlantiğe açılan her bir
şirket gemisi kara kıtaya çok az şey kazandırıken karşılığında ise insanları
başta olmak üzere sömürülecek herşeyi almıştır. Altına ulaşma ve zenginlik
adına yapılan herbir ticari sefer batıya daha fazla refah getirirken kurulan
kalelerde kıtaya adeta birer hançer gibi saplanmıştır. Afrika´nın sömürülmesine
diğer Avrupa ülkelerine göre daha geç başlayan İngilizler ise kurdukları
şirketleri ve faaliyetleri ile bu açığı kapatmıştır. Özellikle kıta insanının
köleleştirilmesinden ise çok büyük kazançlar elde edilmiştir. Bu çalışma
İngiliz ve Afrika tarihi açısından büyük bir öneme sahip Kraliyet Afrikan
Şirketinin faaliyetlerini ortaya çıkarmayı amaçlamıştır. Çalışma sayesinde
kıtanın nasıl sömürüldüğünün daha iyi anlaşılacağı düşünülmektedir.
MAHALLEYE DÖNÜŞEN KÖYLER ÜZERİNE BİR İNCELEME
Authors: AYDIN Fatma , NEGİZ Nilüfer
Published: December 2019

6360 sayılı On Dört İlde Büyükşehir Belediyesi ve Yirmi Yedi İlçe Kurulması ile Bazı Kanun ve
Kanun Hükmünde Kararnamelerde Değişiklik Yapılmasına Dair Kanun ile 30 büyükşehirde
köyler mahalleye dönüşmüştür. Bu dönüşümle kırsal alanı ifade eden ve 442 sayılı Köy
Kanunu’na tabi köyler, tüzel kişiliklerini kaybetmiştir. Köyler mahalle olarak kent yönetimine
dahil olmuştur. Mahalle sayısı artarken köy sayısı azalmıştır. Köylerin mahalleye dönüşmesiyle
gelen değişikliklerle 6360 sayılı Kanun, literatürde geniş yer bulmuştur. Bu çalışmada, kanunun
yeni mahallelere etkisi araştırılmış ve Manisa’nın Köprübaşı ve Selendi ilçesindeki bazı yeni
mahallelerde vatandaşın yönetimden ve sunulan hizmetlerden memnuniyetinin ölçülmesi
amaçlanmıştır. Saha bulguları araştırma evreninde bu kanunun vatandaş tarafından henüz
anlaşılmadığı ve hem hizmet hem de yönetsel açıdan araştırma alanında yetersiz olduğu sonucunu
sunmaktadır.
Students with Socioeconomic Disadvantages who have Academic Success in Language: Examining Academic Resilience in South America
Authors: Salvo-Garrido Sonia , Miranda Vargas Horacio , Vivallo Urra Oscar , Gálvez-Nieto José Luis , Miranda-Zapata Edgardo
Published: December 2019

Framed in a context with an emerging economy and with a high percentage of school failure, this study aimed to identify the factors that turn students with socioeconomic disadvantages into resilient students. Two questions guided the research: Can resilience be supported in students in adverse socioeconomic situations? What factors influence building resilient students? A cross-sectional study was carried out from 2011 to 2015 in Chile, using a multilevel logistic regression model with three levels, considering the hierarchical data structure. The behavior of 63100 to 76400 sampled students was analyzed. Results show five relevant factors in building resilience: self-efficacy in language, minor aggressions and violence perceived by students, norms and values of the establishment, interest in study, and self-efficacy. Some risk factors identified were an atmosphere of less respect and trust, engagement in physical education activities and good performance in carrying them out. These results could orient educational leaders interested in supporting the educational community in order to improve the academic success of disadvantaged students.
Machine Learning for Paper Grammage Prediction Based on Sensor Measurements in Paper Mills
Authors: Abbas Hosny
Published: December 2019

Automation is at the core of modern industry. It aims to increase production rates, decrease production costs, and reduce human intervention in order to avoid human mistakes and time delays during manufacturing. On the other hand, human assistance is usually required to customize products and reconfigure control systems through a special process interface called Human Machine Interface (HMI). Machine Learning (ML) algorithms can effectively be used to resolve this tradeoff between full automation and human assistance. This paper provides an example of the industrial application of ML algorithms to help human operators save their mental effort and avoid time delays and unintended mistakes for the sake of high production rates. Based on real-time sensor measurements, several ML algorithms have been tried to classify paper rolls according to paper grammage in a white paper mill. The performance evaluation shows that the AdaBoost algorithm is the best ML algorithm for this application with classification accuracy (CA), precision, and recall of 97.1%. The generalization of the proposed approach for achieving a cost-effective mill construction by reducing the total number of the required physical sensors will be the subject of our future research.
A Systematic Literature Review of Research Methodologies Used for Evaluation of Augmented Reality Based Learning Applications
Authors: Sirshar Mehreen , Baig Haleema Sadia , Ali Syeda Hafsa
Published: December 2019

With the advancement in AR technology, more education-based applications are being developed using Augmented Reality, which has revolutionized the learning experience. However, in order to determine the application’s impact on student’s motivation, performance and their communication with the lecturer, various studies are conducted. These studies use one of the three research methodologies for data analysis and evaluation. In this systematic review, we have analyzed various research methodologies for system evaluation of the AR learning applications and recorded the student response toward the system. Also, we checked which methodology is preferred by researchers and why. A total number of 25 studies were analyzed which were published during the year of 2015 and 2019. The results indicate that most popular research technique is mixed methodology as it combines both qualitative and quantitative techniques. The purpose of this review is to offer new insights to researchers and provide them with advice about evaluation of AR applications and which tool or technique is more effective.
DIVERSITY OF ENDOPHYTIC FUNGI IN THE ROOT, LEAF, STOLON AND PETIOLE OF ASIATIC PENNYWORT (Centella asiatica)
Authors: Susilowati Dwi Ningsih , Rakhmaniar Amelia , Radiastuti Nani , Roostika Ika
Published: December 2019

Endophytic fungi live in healthy tissues of many plants, including in medicinal plant such as Asiatic pennywort (Centella asiatica). These fungi exist in different parts of the plant as symbionts. The study aimed to isolate endophytic fungi from various parts of Asiatic pennywort of Malaysia accession and characterize their nature.  Three individual plants of Asiatic pennywort (3 months-old) were obtained from the Sringanis Medicinal Garden in Bogor. The endophytes were isolated on Malt Extract Agar. The community structures of the endophytes were analyzed based on their diversity, colonization, dominance index, and relative frequency of occurrence of the isolated endophytic fungi. A total of 78 isolates have been obtained from three individual plants and clustered into 22 morphotypes consisted of 18 morphotypes of Ascomycota and 4 morphotypes of Basidiomycota divisions. The stolons harbored more endophytes (22.9 %) followed by leaf (16.7 %), root (11.8 %), and petiole (7.6 %). The diversity index was classified as medium category with the highest result (1.91) was found in the root, followed by leaf (1.79), stolon (1.75), and petiole (1.29). The most dominant endophytes were identified as Ceratobasidium sp., Colletotrichum sp, and Fusarium sp. Ceratobasidium sp. has the highest dominance index (0.02). UPGMA cluster analysis grouped the endophytic fungi into distinct clusters based on the plant parts origin. This study implied that stolon was the the most suitable part of Asiatic pennywort for isolating endophytic fungi. Further study is required to examine the role of the endophytic fungi to produce secondary metabolites in Asiatic pennywort.
Analysis of the role of TpUB05 antigen from Theileria parva in immune responses to malaria in humans compared to its homologue in Plasmodium falciparum; UB05 antigen
Authors: Dinga Jerome Nyhalah , Gamua Stanley Dobgima , Perimbie Stephanie Numenyi , Chuma Francis N. G. , Njimoh Dieudonné Lemuh , Djikeng Apollinaire , Pelle Roger , Titanji Vincent P. K.
Published: December 2019

Background: Despite the amount of resources deployed and technological advancements in Molecular Biology, vaccinology, immunology, genetics, and biotechnology, there is still no effective vaccines against malaria. Immunity to either malaria or East Coast fever is usually seen as species- and/or strain-specific. But there is growing body of evidence suggesting the possibility of the existence of cross strain, cross species and cross genus immune responses in apicomplexans. The principle of gene conservations indicates that homologues play similar role in closely related organisms. UB05 antigen (XP_001347656.2) from P. falciparum is part of chimeric UB05-09 antigen; a potential vaccine candidate has been demonstrated to be a marker of protective immunity in malaria. The homologue of UB05 in T. parva is TpUB05 (XP_763711.1) which was also tested and shown to be a potential marker of protective immunity in ECF as well. In a bid to identify potent markers of protective immunity to aid malaria vaccine development, TpUB05 was tested in malaria caused by P. falciparum .
Results: It was observed that TpUB05 provoked stronger immune responses in malaria compared to UB05 antigen as tested using ELISA, ex-vivo ELISpot assay and in vitro growth inhibition assay.
Conclusion: This study suggests for the first time that TpUB05 from T. parva is a better marker of protective immunity in malaria compared to its homologue UB05 from P. falciparum .
Coxiella burnetii is widespread in ticks (Ixodidae) in the Xinjiang border areas of China
Authors: Ni Jun , Lin Hanliang , Xu Xiaofeng , Ren Qiaoyun , Aizezi Malike , Luo Jin , Luo Yi , Ma Zhan , Chen Ze , Tan Yangchun , Guo Junhui , Liu Wenge , Qu Zhiqiang , Wu Zegong , Wang Jinming , Li Youquan , Guan Guiquan , Luo Jianxun , Yin Hong , Liu Guangyuan
Published: December 2019

Background The gram-negative Coxiella burnetii bacterium is the pathogen that causes Q fever. The bacterium is transmitted to animals via ticks and can cause infection in domestic animals, wild animals, and humans. As the provincial-level administrative region with the largest land area in China, Xinjiang has many endemic tick species; however, the distribution of C. burnetii in ticks in Xinjiang border areas has not been studied in detail.Results For the current study, 1507 ticks were collected from livestock at 22 sampling sites in ten border regions of the Xinjiang Uygur Autonomous region from 2018 to 2019. C. burnetii was detected in 205/348 (58.91%) Dermacentor nuttalli ; in 110/146 (75.34%) Dermacentor pavlovskyi ; in 66/80 (82.50%) Dermacentor silvarum ; in 15/32 (46.90%) Dermacentor niveus ; in 28/132 (21.21%) Hyalomma rufipes ; in 24/25 (96.00%) Hyalomma anatolicum ; in 219/312 (70.19%) Hyalomma asiaticum ; in 252/338 (74.56%) Rhipicephalus sanguineus ; and in 54/92 (58.70%) Haemaphysalis punctata . Among these samples, C. burnetii was detected in D. pavlovskyi for the first time. The infection rate of R. sanguineus was 74.56% (252/338), which was the highest among the four tick genera sampled, whereas the infection rate of H. anatolicum was 96% (24/25), which was the highest among the nine tick species sampled. A sequence analysis indicated that 63 16S rRNA sequences could be found in four newly established genotypes: CXJ-1 (n = 18), CXJ-2 (n = 33), CXJ-3 (n = 6), and CXJ-4 (n = 6).Conclusions This study indicates that CXJ-2 might represent the main C. burnetii genotype in the ticks in Xinjiang because it was detected in eight of the tick species studied. The high infection rate of C. burnetii detected in the ticks found in domestic animals may indicate a high likelihood of Q fever infection in both domestic animals and humans.
Identification and validation of a prognostic index based on a metabolic-genomic landscape analysis of ovarian cancer
Authors: Wang Zi-yao , Li Yukun , Luo Gui-fang , Zou Juan , Chen Chang-ye , Wang Juan , Zeng Xin , Xiao Jiao , Tong Wen-juang , Ma Yan
Published: December 2019

Purpose: Tumor metabolism has been a novel driver of personalized cancer medicine, with aggressive efforts to regulate the metabolic system to prolong their life. The aim of this study is to explore the prognostic value of metabolism in ovarian serous cystadenocarcinoma (OSC), which is the most common subtype of ovarian cancer, accounts for 75-80% of reported cases. Patients and methods: we integrated the expression profiles of metabolism-related genes (MRGs) in survival in 379 OSC patients based on The Cancer Genome Atlas (TCGA) database. Then, several biomedical computational algorithms were employed to identify eight key prognostic MRGs, which were related with overall survival (OS) significantly in OSC. The eight genes represented important clinical significance and prognostic value in OSC. Then a prognostic index was constructed. Results: A total of 701 differentially expressed metabolism-related genes (MRGs) were identified in OSC patients based on TCGA database. Functional enrichment analyses hinted that metabolism may act in a significant role in the development and progression of OSC. Random walking with restart (RWR) algorithm, Univariate cox and lasso regression analysis indicated a prognostic signature based on MRGs (ENPP1, FH, CYP2E1, HPGDS, ADCY9, NDUFA5, ADH1B and PYGB), which performed moderately in prognostic predictions. Conclusion: This study provides a latent prognostic feature for predicting the prognosis of OSC patients and the molecular mechanism of OSC metabolism.
Additional clinical benefits of probiotics as an adjunctive therapy to nonsurgical periodontal treatment of periodontitis: a systematic review and meta-analysis
Authors: Gou Huiqing , Chen Xu , Li Lu , Wang Xiaoqian , Xu Yan
Published: December 2019

Background: With more and more concerns concentrated on the novel therapy applying probiotics, we challenge its trustworthy clinical efficacy as an adjuvant to scaling and root planning (SRP) as compared with SRP alone or combined with placebo or other nonsurgical treatment applied as the initial therapy of periodontitis. Methods: Electronic databases retrieval was performed until October 2017 under certain screening condition. Clinical randomized controlled trials (RCTs) to assess the efficacy of SRP + probiotic versus SRP were included. Primary outcome variables were PPD (pocket probing depth) reduction and CAL (clinical attachment level) gain. Nine publications were eligible for the systematic review and three were evaluated in the meta-analysis. Results: Results demonstrated statistically significant bleeding on probing (BOP) percentage reduction (5.34, p< 0.00001) at short-term, but not a significant differences of overall PPD reduction (0.23mm, p=0.25) for SRP + probiotic treatment versus SRP at short-term, and there was only a tendency (p = 0.08) with regard to overall CAL gain. However significant reduction of PPD and gain of CAL were observed when stratified for deep pockets (0.61mm, p< 0.00001) and for moderate pockets (0.37mm, p=0.006) at short-term respectively. Conclusions: Within the ranges of this study, the adjunctive use of probiotics seem to achieve short-term clinical benefits in the treatment of periodontitis. Conclusions must be treated with caution and future long-term RCTs are needed to testify the clinical application value of probiotics.
Effects of pulsed in-feed antimicrobials on chicken fecal resistome
Authors: Chen Siyuan , Yang Jintao , Zeng Jiaxiong , Hao Jie , Zhao Ruonan , Zeng Zhenling , Xiong Wenguang
Published: December 2019

Background: Antimicrobial resistance has become a global problem that poses great threats to human health. Antimicrobials are widely used in broiler chicken production and consequently affect the fecal resistome. Here we used metagenomic approach to investigate the effects of pulsed antimicrobial administration on chicken fecal resistome. Chickens received three 5-day-course of alone/combined antimicrobials at therapeutic dose, including amoxicillin, chlortetracycline and florfenicol. Chicken feces was collected on T0 (before treatment), T5 (after first treatment), T10 (after second treatment), T15 (after third treatment) at the chicken age of 40 days that are ready for slaughter. Results: Tetracycline resistance gene and polymyxin resistance gene only responded to chlortetracycline and amoxicillin, respectively. The tetracycline resistance gene tetC significantly increased from T0 to T15 in chlortetracycline group. The multidrug resistance gene acrB, acrF, mdtA, mdtK and CRP only occurred significant increase in amoxicillin group (P<0.05). Klebsiella significantly decreased from T0 to T10 in florfenicol group, while significantly increased from T0 to T15 in chlortetracycline group (P<0.05). Amoxicillin did not lead significant change to Klebsiella. Escherichia was always the major host for multidrug resistance genes. ARG-harboring Escherichia increased mainly due to the antimicrobial selection for β-lactam, tetracycline, chloramphenicol resistance genes they harbored. Conclusions: The results indicated that the effects induced by amoxicillin, chlortetracycline and florfenicol significantly shaped the fecal microbiome and resistome. We provided a comprehensive insight into antimicrobial-mediated alteration of chicken fecal microbiome and resistome. These findings will give suggests to veterinarian for combating antimicrobial resistance in broiler chicken production.
The effectiveness of an independent anti-neoplastic medication therapy management system
Authors: Zhang Jianping , Xu Rong , Zhao Xincai , Wang Yonggang , Zhu Wanhu , Xiao Misu , Hu Haiyan , Tang Lina , Guo Cheng , Shen Zan
Published: December 2019

Background: Cancer has been a serious health threat for human especially in developing countries. As the tumor patients are largely in elderly population, the ongoing trend of population aging and increased long-term cancer survivors will add complexity to tumor patients management. However, the tumor patients, aged and with multiple therapeutics, are largely under-served in medication service in China. The aim of this study was to evaluated the effectiveness of an independent anti-neoplastic medication therapy management system for tumor patients management.Methods: An independent, anti-neoplastic medication therapy management (MTM) system, in Shanghai Jiao Tong University affiliated sixth People's Hospital, was established in 2018 as a result of collaboration of onco-chemotherapy, clinical pharmacy and software engineers. The system consists of an independent clinic of pharmacy and MTM software. The software consisted of six modules to enable clinical pharmacists to serve the patients. The six modules are medication therapy review, intervention plan, personal medication record, medication-related action plan, intervention and/or referral, and documentation and follow-up.Results: In a year, 173 tumor patients visited the pharmacy clinic and the averaged clinic visiting count was 2.4 for the all tumor patients. Two thirds patients (117/173) were with one or more identified drug-related problems (DRPs) in medication therapy review. Three leading DRPs are adverse drug reaction, potential drug-drug interaction and non-adherence. Seventy percent of DRPs could be resolved (cure or improved) in four weeks; the medication adherence reached 84%-100% following three or four round of review and intervention.Conclusions: The involvement of clinical pharmacists in polypharmacy tumor patients management increased the communication with oncologists, and could ultimately enhance the effectiveness, safety and rational use of medication.
A post-method condition analysis of using ensemble machine learning for cancer prognosis and diagnosis: a systematic review
Authors: Mirsadeghi Leila , Banaei-Moghaddam Ali Mohammad , Beh-Afarin Seyed Reza , Hosseini Reza Haji , Kavousi Kaveh
Published: December 2019

Background: Ensemble methods are supervised learning approaches that integrate different types of data or multiple individual classifiers. It has been shown that these methods can improve professional performance.Methods: This study is an attempt to provide an in-depth review on 45 most relevant articles and aims to introduce 42 ensemble classifier (EC) machine learning methods used for the detection of 18 different types of cancer. Compared to other types of cancer, breast cancer, and the 22 ensemble methods introduced for its identification, is extensively investigated. The purpose of this study is to identify, map, and analyze the current academic discourse on EC machine learning methods in order to: 1. identify overarching themes emerging from empirical studies as regards EC methods, 2. determine their input data and decision-making strategies, and 3. evaluate relevant statistical procedures.Results: By comparing various approaches, we can introduce Relevance Vector Machine (RVM)-based ensemble learning method that can provide optimal solutions for problems such as curse the dimensionality and high-dimensionality of feature space without missing data values.Conclusions: To obtain robust performance and achieve better results, it is tactfully suggested to use multi-omics data integration, which has demonstrated to identify cancers and their subtypes more efficiently.
Monocentric 8-year clinical follow-up of cladribine-treated patients from registration trials
Authors: Moccia Marcello , Lanzillo Roberta , Petruzzo Martina , Nozzolillo Agostino , Angelis Marcello De , Carotenuto Antonio , Palladino Raffaele , Morra Vincenzo Brescia
Published: December 2019

The authors have requested removal of this preprint.
Hcfc1a regulates neural precursor proliferation and asxl1 expression in the developing brain
Authors: Castro Victoria L. , Reyes Joel F. , Reyes-Nava Nayeli , Paz David , Quintana Anita M
Published: December 2019

Background: Precise regulation of neural precursor cell (NPC) proliferation and differentiation is essential to ensure proper brain development and function. The HCFC1 gene encodes a transcriptional co-factor that regulates cell proliferation, and previous studies suggest that HCFC1 regulates NPC function. However, the molecular mechanism underlying these cellular deficits has not been completely characterized. Methods: Here we created a zebrafish harboring mutations in the hcfc1a gene (the hcfc1aco60/+ allele), one ortholog of HCFC1, and utilized immunohistochemistry and RNA-sequencing technology to understand the function of hcfc1a during neural development.Results: The hcfc1aco60/+ allele results in an increased number of NPCs, neurons, and radial glial cells. These deficits are associated with the abnormal expression of asxl1, a polycomb transcription factor, which we identified as a downstream effector of hcfc1a using high throughput RNA sequencing technology. Inhibition of asxl1 activity and/or expression in larvae harboring the hcfc1aco60/+ allele completely restored the number of NPCs to normal levels. Conclusion: Collectively, our data demonstrate a novel pathway in which hcfc1a regulates NPCs and neurogenesis.
Bjerkandera adusta M1 inhibits Fusarium oxysporum and prevents the wilt incidence in Brassica napus
Authors: feng xiao , Li Yong , Li Suping , Lu Yifan , Zhang Jingjie , Zhu Yunyun , Yang Hongjun , He Xinhua
Published: December 2019

Background: Bjerkandera adusta degrades polycyclic aromatic compounds, such as cellulose and lignin, with the production of laccase and peroxidase. However, its effect on plant disease is unknown. Results: In this study, both the confrontation culture and greenhouse pot experiments were carried out to address the biocontrol mechanisms of B. adusta M1, which was isolated from a unique purple soil (Eutric Regosol), on the growth of pathogenic Fusarium oxysporum f. sp. conglutinans (FOC) and incidence of fusarium wilt in Brassica napus. Results showed that the hyphal growth rate of B. adusta M1 was significantly greater than that of FOC, indicating a strong competitiveness by B. adusta M1. In addition, the B. adusta M1 fermentation broth significantly inhibited the growth of FOC hyphae by 62.79±1.80%, which was greater than an inhibition rate of 40.63±1.68% by the chemical fungicide carbendazim. The image from a scanning electron microscope showed the hyphae of FOC directly was penetrated by the B. adusta M1 hyphae, indicating a strong mycoparasitism by B. adusta M1. Besides, both the B. adusta M1 and B. adusta M1 fermentation broth reduced the incidence and disease index of the fusarium wilt in Brassica napus leaves, and the control effects of different treatments against fusarium wilt were 57.09% and 47.67%, respectively, which were comparable better to 46.11% of the chemical fungicide carbendazim. Furthermore, both the B. adusta M1 and B. adusta M1 fermentation broth increased the activity of superoxide dismutase, catalase, peroxidase and phenylalanine ammonia-lyase, which related to an enhancement of disease resistance. Similarly, both the B. adusta M1 and B. adusta M1 fermentation broth decreased the cell membrane permeability and malondialdehyde contents, thereby reducing the cell membrane damage from the pathogenic fungus. Conclusion: In summary, results from this present study demonstrated that both the Bjerkandera adusta M1 and B. adusta M1 fermentation broth inhibited the growth of FOC and decreased the incidence and disease index of the fusarium wilt disease in Brassica napus. Therefore, B. adusta M1 could be applied as a potential biocontrol fungus to against the fusarium wilt disease.
Cerebral palsy needs a review: a novel DDX3X mutation in a family
Authors: Hu Liqin , Xin Xiaoqin , Lin Shaobin , Luo Min , Chen Junkun , Qiu Hongsheng , Ma Li , Huang Jungao
Published: December 2019

Cerebral palsy (CP) is a non-progressive disorder of movement and posture due to a static insult to the brain. Some conditions of other disorders can mimic CP, and thus CP needs to be pursued for its underlying cause. In this paper, we reported a novel DDX3X mutation in a girl afflicted with MRX102 which had been confused with CP in her early life. The application of whole exome sequencing may be the best method to distinguish between actual CP and other disorders with similar characteristics. A thorough and conscientious review is suggested during the diagnostic progress.
To cope with drought: two forage grass species – Festuca arundinacea and F. glaucescens can activate similar survival strategies although they differ with molecular response
Authors: Lechowicz Katarzyna , Pawlowicz Izabela , Perlikowski Dawid , Arasimowicz-Jelonek Magdalena , Majka Joanna , Augustyniak Adam , Kosmala Arkadiusz
Published: December 2019

Background: Photosynthesis is among the primary processes affected by drought and its disturbances result with the reduction of growth, reactive oxygen species (ROS) overproduction, and alterations in antioxidant system activity. Our study was performed on two closely related forage grasses: Festuca arundinacea and F. glaucescens. Two genotypes within each species significantly differing with the potential of drought resistance: high drought resistant (HDR) and low drought resistant (LDR), were used. The research involved: (i) the analysis of gene expression at transcript and protein levels for the selected enzymes of Calvin cycle (fructose-1,6-bisphosphate aldolase (pFBA), phosphoglycerate kinase (PGK) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH)) and (ii) the activity of pFBA, as a protein marker of the Calvin cycle, (iii) the analysis of gene expression at protein level for the selected antioxidant enzymes (glutathione reductase (GR), glutathione peroxidase (GPX), L-ascorbate peroxidase (APX), catalase (CAT), and superoxide dismutase (SOD)), (iv) the measurements of physiological parameters describing a plant’s physiological status under control, drought and re-watering conditions (relative water content (RWC), electrolyte leakage (EL), lipid peroxidation, chlorophyll fluorescence, gas exchange, and ROS level).Results: Our analysis clearly showed that physiological reactions to water deficit were similar in both HDR and both LDR genotypes of Festuca arundinacea and F. glaucescens but the species revealed significant differences in the potential to tolerate tissue dehydration, what was correlated with distinct expression of the Calvin cycle enzymes under drought stress.Conclusions: The maintenance of stable efficiency of dark phase of photosynthesis seems to be crucial for drought tolerance and recovery in F. glaucescnes, whereas acquisition of drought tolerance in F. arundinacea and F. glaucescens does not involve marked changes at the protein level in the enzymatic antioxidant system.
A qualitative exploration of the experience and quality of life of patients, and their family carers, admitted to a specialist eye hospital with microbial keratitis
Authors: Tranter Shelley Anne , Careras-Aguas Maria , Riddell Mandy , McCulloch Joanna , Riley Therese , Watson Stephanie , Perry Lin
Published: December 2019

Background Microbial keratitis is a vision-threatening condition requiring intensive treatment. Understanding patients’ and carers’ experience during and after hospitalisation can inform improvements in care and outcomes. This study explored the vision-related quality of life, and the experiences of patients with microbial keratitis and their family carers when admitted to a quaternary referral eye hospital in Australia Methods The study employed mixed methods, including qualitative interviews and a survey in hospital and a telephone interview post-discharge. A convenience sample was recruited of 33 patients with microbial keratitis who presented to hospital between March and October 2017, and 10 of their family carers. Semi-structured interviews were audiotaped, transcribed verbatim, coded and analysed using thematic analysis. Patient participants completed the National Eye Institute Visual Functioning Questionnaire – 25 (NEIVFQ-25). Results Qualitative analyses identified two main themes: Saving sight, with subthemes of costs of saving sight, and travel and transportation; Safe-guarding home and normal life with subthemes of family, work and pastimes. A group mean NEIVFQ-25 score of 74 was similar to other ophthalmic disease groups but pain scores were higher. Conclusion Findings provide insights into the experiences and often unspoken concerns of microbial keratitis patients and their family carers, revealing the priority and the associated costs of saving sight, and the implications for family and lifestyles. These patients reported similarly reduced vision-related quality of life but greater pain compared to other ophthalmic groups. Findings point to ways to improve their experience and potentially reduce the high rates of unplanned representations of this patient group.