bluuebunny/crossref_metadata_2025 huggingface dataset:
Zinc Oxide Nanoconjugates against Brain-Eating Amoebae
Authors: Siddiqui Ruqaiyyah , Boghossian Anania , Akbar Noor , Jabri Tooba , Aslam Zara , Shah Muhammad Raza , Alharbi Ahmad M. , Alfahemi Hasan , Khan Naveed Ahmed
Published: September 2022

Naegleria fowleri and Balamuthia mandrillaris are opportunistic protists, responsible for fatal central nervous system infections such as primary amoebic meningoencephalitis (PAM) and granulomatous amoebic encephalitis (GAE) with mortality rates higher than 90%. Threatening a rise in cases is the increase in temperature due to global warming. No effective treatment is currently available. Herein, nanotechnology was used to conjugate Zinc oxide with Ampicillin, Ceftrixon, Naringin, Amphotericin B, and Quericitin, and the amoebicidal activity and host cell cytotoxicity of these resulting compounds were investigated. The compounds ZnO-CD-AMPi, ZnO-CD-CFT, ZnO-CD-Nar, ZnO-CD-AMB, and ZnO-CD-QT were found to reduce N. fowleri viability to 35.5%, 39.6%, 52.0%, 50.8%, 35.9%, and 69.9%, respectively, and B. mandrillaris viability to 40.9%, 48.2%, 51.6%, 43.8%, and 62.4%, respectively, when compared with their corresponding controls. Furthermore, the compounds reduced N. fowleri-mediated and B. mandrillaris-mediated host cell death significantly. Additionally, the compounds showed limited cytotoxicity against human cells; cell toxicity was 35.5%, 36.4%, 30.9%, 36.6%, and 35.6%, respectively, for the compounds ZnO-CD-AMPi, ZnO-CD-CFT, ZnO-CD-Nar, ZnO-CD-AMB, and ZnO-CD-QT. Furthermore, the minimum inhibitory concentrations to inhibit amoeba growth by 50% were determined for N. fowleri and B. mandrillaris. The MIC50 for N. fowleri were determined to be 69.52 µg/mL, 82.05 µg/mL, 88.16 µg/mL, 95.61 µg/mL, and 85.69 µg/mL, respectively; the MIC50 of the compounds for B. mandrillaris were determined to be 113.9 µg/mL, 102.3 µg/mL, 106.9 µg/mL, 146.4 µg/mL, and 129.6 µg/mL, respectively. Translational research to further develop therapies based on these compounds is urgently warranted, given the lack of effective therapies currently available against these devastating infections.
Improved Antioxidative, Anti-Inflammatory, and Antimelanogenic Effects of Fermented Hydroponic Ginseng with Bacillus Strains
Authors: Park Ji-Young , Song Myung Wook , Kim Kee-Tae , Paik Hyun-Dong
Published: September 2022

Compared with traditionally cultured ginseng, hydroponic ginseng (HG) contains more remarkable bioactive compounds, which are known to exert diverse functional effects. This study aimed to enhance the multifunctional effects, including the antioxidative, anti-inflammatory, and antimelanogenic effects, exhibited by fermented HG with Bacillus strains, such as Bacillus subtilis KU43, Bacillus subtilis KU201, Bacillus polyfermenticus SCD, and Bacillus polyfermenticus KU3, at 37 °C for 48 h. After fermentation by B. subtilis KU201, the antioxidant activity, determined using ABTS and FRAP assays, increased from 25.30% to 51.34% and from 132.10% to 236.27%, respectively, accompanied by the enhancement of the phenolic compounds and flavonoids. The inflammation induced in RAW 264.7 cells by lipopolysaccharide (LPS) was ameliorated with fermented HG, which regulated the nitric oxide (NO), prostaglandin E2 (PGE2), and proinflammatory markers (tumor necrosis factor (TNF)-α, and interleukin (IL)-1β and IL-6). The treatment with fermented HG inhibited the melanin accumulation in B16F10 cells induced by α-melanocyte-stimulating hormone (α-MSH) by controlling the concentrations of melanin synthesis and tyrosinase activity. These results indicate that the HG exhibited stronger antioxidative, anti-inflammatory, and antimelanogenic effects after fermentation. Consequently, HG fermented by Bacillus strains can potentially be used as an ingredient in cosmetological and pharmaceutical applications.
Antioxidant, Anti-Inflammatory, and Antibacterial Properties of an Achillea millefolium L. Extract and Its Fractions Obtained by Supercritical Anti-Solvent Fractionation against Helicobacter pylori
Authors: Villalva Marisol , Silvan Jose Manuel , Alarcón-Cavero Teresa , Villanueva-Bermejo David , Jaime Laura , Santoyo Susana , Martinez-Rodriguez Adolfo J.
Published: September 2022

The main objective of this work is to evaluate the potential utility of an Achillea millefolium extract (yarrow extract, YE) in the control of H. pylori infection. The supercritical anti-solvent fractionation (SAF) process of YE allowed the obtaining of two different fractions: yarrow’s precipitated fraction (YPF), enriched in most polar phenolic compounds (luteolin-7-O-glucoside, luteolin, and 3,5-dicaffeoylquinic acid), and yarrow’s separator fraction (YSF), enriched in monoterpenes and sesquiterpenes, mainly containing camphor, artemisia ketone, and borneol. YE was effective in reducing reactive oxygen species (ROS) production in human gastric AGS cells by 16% to 29%, depending on the H. pylori strain. YPF had the highest inhibitory activity (38–40%) for ROS production. YE modulated the inflammatory response in AGS gastric cells, decreasing IL-8 production by 53% to 64%. This IL-8 inhibition also showed a strain-dependent character. YPF and YSF exhibited similar behavior, reducing IL-8 production, suggesting that both phenolic compounds and essential oils could contribute to IL-8 inhibition. YSF showed the highest antibacterial activity against H. pylori (6.3–7.1 log CFU reduction, depending on the strain) and lower MIC (0.08 mg/mL). Results obtained have shown that YE and SAF fractions (YPF and YSF) were effective as antioxidant, anti-inflammatory, and antibacterial agents regardless of the H. pylori strain characteristics.
Proteometabolomic Analysis Reveals Molecular Features Associated with Grain Size and Antioxidant Properties amongst Chickpea (Cicer arietinum L.) Seeds Genotypes
Authors: Quiroz-Figueroa Francisco R. , Monribot-Villanueva Juan L. , Bojórquez-Velázquez Esaú , Gómez-Peraza Rosa L. , Elizalde-Contreras José M. , Bautista-Valle Mirna V. , Guerrero-Analco José A. , Valdez-Morales Maribel , Singh Rupesh Kumar , Ruiz-May Eliel
Published: September 2022

Legumes are an essential source of nutrients that complement energy and protein requirements in the human diet. They also contribute to the intake of bioactive compounds such as polyphenols, whose content can vary depending on cultivars and genotypes. We conducted a comparative proteomics and metabolomics study to determine if there were significant variations in relevant nutraceutical compounds in the five genotypes of Kabuli-type chickpea grains. We performed an isobaric tandem mass tag (TMT) couple to synchronous precursor selection (SPS)-MS3 method along with a targeted and untargeted metabolomics approach based on accurate mass spectrometry. We observed an association between the overproduction of proteins involved in starch, lipid, and amino acid metabolism with gibberellin accumulation in large grains. In contrast, we visualized the over-accumulation of proteins associated with water deprivation in small grains. It was possible to visualize in small grains the over-accumulation of some phenolics such as vanillin, salicylic acid, protocatechuic acid, 4-coumaric acid, 4-hydroxybenzoic acid, vanillic acid, ferulic acid, and kaempferol 3-O-glucoside as well as the amino acid l-phenylalanine. The activated phenolic pathway was associated with the higher antioxidant capacity of small grains. Small grains consumption could be advantageous due to their nutraceutical properties.
Synthesis of Zinc Oxide Nanoparticles with Bioflavonoid Rutin: Characterisation, Antioxidant and Antimicrobial Activities and In Vivo Cytotoxic Effects on Artemia Nauplii
Authors: Saleemi Mansab Ali , Alallam Batoul , Yong Yoke Keong , Lim Vuanghao
Published: September 2022

This study aims to synthesise zinc oxide nanoparticles with rutin (ZnO-R NPs) using a green synthesis approach and characterise the nanostructures for diverse biomedical applications. In this study, the optical and chemical properties of synthesised ZnO-R NPs were verified through Fourier transform infrared (FTIR) spectroscopy and ultraviolet-visible (UV-Vis) spectroscopy. The FTIR spectroscopy revealed a symmetric bending vibration peak of 460 cm−1 for ZnO-R NPs, whereas UV-Vis spectroscopy showed a distinct absorption band at 395 nm. Moreover, the oval-shaped morphology of ZnO-R NPs was verified through scanning electron microscopy and transmission electron microscopy. The synthesised nanoformulation revealed a wurtzite structure with a crystallite size of 13.22 nm; however, the zeta potential value was recorded as −8.50 ± 0.46 mV for ZnO-R NPs. According to an antioxidant study, ZnO-R NPs demonstrated lower free-radical scavenging activity than pure rutin. The cytotoxicity study was conducted using a human breast cancer cell line (MCF-7). In vitro analysis verified that ZnO-R NPs exhibited significantly higher anticancer and microbial growth inhibition activities than standard ZnO NPs (ZnO Std NPs) and pure rutin. In addition, ZnO-R NPs revealed a significantly lower IC50 value than the commercial ZnO Std NPs and pure rutin in MCF-7 cells (16.39 ± 6.03 μg/mL, 27 ± 0.91 μg/mL and 350 ± 30.1 μg/mL, respectively) after 48 h. However, synthesised ZnO-R NPs demonstrated no significant toxicity towards Artemia nauplii. These results highlight the synthesis of rutin-mediated ZnO NPs and their possible chemotherapeutic potential.
Artemisinin Targets Transcription Factor PDR1 and Impairs Candida glabrata Mitochondrial Function
Authors: Zhu Pan , Yue Chaoping , Zeng Xin , Chen Xiulai
Published: September 2022

A limited number of antifungal drugs, the side-effect of clinical drugs and the emergence of resistance create an urgent need for new antifungal treatment agents. High-throughput drug screening and in-depth drug action mechanism analyzation are needed to address this problem. In this study, we identified that artemisinin and its derivatives possessed antifungal activity through a high-throughput screening of the FDA-approved drug library. Subsequently, drug-resistant strains construction, a molecular dynamics simulation and a transcription level analysis were used to investigate artemisinin’s action mechanism in Candida glabrata. Transcription factor pleiotropic drug resistance 1 (PDR1) was an important determinant of artemisinin’s sensitivity by regulating the drug efflux pump and ergosterol biosynthesis pathway, leading to mitochondrial dysfunction. This dysfunction was shown by a depolarization of the mitochondrial membrane potential, an enhancement of the mitochondrial membrane viscosity and an upregulation of the intracellular ROS level in fungi. The discovery shed new light on the development of antifungal agents and understanding artemisinin’s action mechanism.
Insight into the Mechanism of Salt-Induced Oxidative Stress Tolerance in Soybean by the Application of Bacillus subtilis: Coordinated Actions of Osmoregulation, Ion Homeostasis, Antioxidant Defense, and Methylglyoxal Detoxification
Authors: Hasanuzzaman Mirza , Raihan Md. Rakib Hossain , Nowroz Farzana , Fujita Masayuki
Published: September 2022

Considering the growth-promoting potential and other regulatory roles of bacteria, we investigated the possible mechanism of the role of Bacillus subtilis in conferring salt tolerance in soybean. Soybean (Glycine max cv. BARI Soybean-5) seeds were inoculated with B. subtilis, either through a presoaking with seeds or a direct application with pot soil. After 20 days of sowing, both the seed- and soil-inoculated plants were exposed to 50, 100, and 150 mM of NaCl for 30 days. A clear sign of oxidative stress was evident through a remarkable increase in lipid peroxidation, hydrogen peroxide, methylglyoxal, and electrolyte leakage in the salt treated plants. Moreover, the efficiency of the ascorbate (AsA)–glutathione (GSH) pathways was declined. Consequently, the plant growth, biomass accumulation, water relations, and content of the photosynthetic pigments were decreased. Salt stress also caused an increased Na+/K+ ratio and decreased Ca2+. On the contrary, the B. subtilis inoculated plants showed increased levels of AsA and GSH, their redox balance, and the activities of the AsA–GSH pathway enzymes, superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase, and peroxidase. The B. subtilis inoculated plants also enhanced the activities of glyoxalase enzymes, which mitigated methylglyoxal toxicity in coordination with ROS homeostasis. Besides this, the accumulation of K+ and Ca2+ was increased to maintain the ion homeostasis in the B. subtilis inoculated plants under salinity. Furthermore, the plant water status was uplifted in the salt treated soybean plants with B. subtilis inoculation. This investigation reveals the potential of B. subtilis in mitigating salt-induced oxidative stress in soybean plants through modulating the antioxidant defense and glyoxalase systems along with maintaining ion homeostasis and osmotic adjustments. In addition, it was evident that the soil inoculation performed better than the seed inoculation in mitigating salt-induced oxidative damages in soybean.
Low HDL Cholesterol Efflux Capacity Indicates a Fatal Course of COVID-19
Authors: Stadler Julia T. , Mangge Harald , Rani Alankrita , Curcic Pero , Herrmann Markus , Prüller Florian , Marsche Gunther
Published: September 2022

Plasma membrane cholesterol is required for proper trafficking and localization of receptors that facilitate severe acute respiratory syndrome coronavirus 2 infection. High-density lipoproteins (HDL) mobilize plasma membrane cholesterol, and HDL-cholesterol levels are associated with the severity of COVID-19 disease and mortality. However, HDL-cholesterol levels poorly reflect the function of this complex family of particles, and a detailed assessment of COVID-19-associated changes in HDL functionality and its prognostic value is lacking. In the present study, we assessed HDL cholesterol efflux capacity, HDL anti-inflammatory and antioxidant properties, and changes in HDL composition and metabolism in COVID-19 (n = 48) and non-COVID pneumonia patients (n = 32). COVID-19 infection markedly reduced the activity of lecithin-cholesteryl-acyltransferase and functional parameters of HDL, such as the cholesterol efflux capacity, arylesterase activity of paraoxonase 1, and anti-oxidative capacity of apoB-depleted serum when compared to non-COVID pneumonia at baseline, paralleled by markedly reduced levels of HDL-cholesterol. Of particular interest, low HDL cholesterol efflux capacity was associated with increased mortality risk in COVID-19 patients, independent of HDL-C levels. Our results highlight profound effects of COVID-19 infection on HDL function, metabolism, and composition. Low HDL cholesterol efflux capacity indicates a fatal course of COVID-19, independent of HDL-cholesterol levels.
Probabilistic Analysis of Ground Surface Settlement of Excavation Considering Spatial Variable Modified Cam-Clay Model Parameters
Authors: Cheng Hao , Chen Hui , Jia Hanying , Zhang Shu , Liu Xiao
Published: September 2022

The modified Cam-clay model (MCC model) is capable of representing the consolidation process of the soil under a complex stress path and thus is extensively adopted in the numerical analysis of excavation engineering. For reliability problems of ground surface settlement of excavation, minimal attention has been paid to investigating the effect of spatial variable MCC model parameters on the settlement during the staged construction of the excavation. Based on laboratory tests and sensitivity analysis, this study launched the probabilistic transient hydro-mechanical coupling analysis of excavation settlement considering spatial variable deformation parameters (λ and κ) using an MCS-FORM hybrid approach. The results show that: (1) Both the deformation parameters have a positive relationship with the settlement, and κ has a more significant effect than λ. (2) The observed maximum settlement and the location with maximum settlement follow a log-normal distribution. (3) An increasing COV of parameters leads to an enhanced surface settlement, expansion of the significant influence region, and decreased reliability. (4) The reliability index is greatly enhanced with the delimited controlled standard value of the surface settlement, Hcon, and it decreases significantly at the preliminary stage and then decreases progressively until stable during excavating. Overall, adopting the deterministic analysis without considering the spatial variability of MCC parameters leads to underestimating the risk due to the settlement and the significant influence region.
Neuro-Symbolic Word Embedding Using Textual and Knowledge Graph Information
Authors: Oh Dongsuk , Lim Jungwoo , Lim Heuiseok
Published: September 2022

The construction of high-quality word embeddings is essential in natural language processing. In existing approaches using a large text corpus, the word embeddings learn only sequential patterns in the context; thus, accurate learning of the syntax and semantic relationships between words is limited. Several methods have been proposed for constructing word embeddings using syntactic information. However, these methods are not trained for the semantic relationships between words in sentences or external knowledge. In this paper, we present a method for improved word embeddings using symbolic graphs for external knowledge and the relationships of the syntax and semantic role between words in sentences. The proposed model sequentially learns two symbolic graphs with different properties through a graph convolutional network (GCN) model. A new symbolic graph representation is generated to understand sentences grammatically and semantically. This graph representation includes comprehensive information that combines dependency parsing and semantic role labeling. Subsequently, word embeddings are constructed through the GCN model. The same GCN model initializes the word representations that are created in the first step and trains the relationships of ConceptNet using the relationships between words. The proposed word embeddings outperform the baselines in benchmarks and extrinsic tasks.
Study of the Decomposition of N-Nitrosonornicotine (NNN) under Inert and Oxidative Atmospheres: Effect of the Addition of SBA-15 and MCM-41
Authors: Asensio Javier , Beltrán María Isabel , Juárez-Serrano Nerea , Berenguer Deseada , Marcilla Antonio
Published: September 2022

Nowadays, the use of tobacco biomass as an energy source is being valued. Therefore, it is important to know the processes that take place during combustion and pyrolysis, as well as the substances that are formed. In this work, we study the compounds obtained during the decomposition of NNN as a function of temperature under inert and oxidant atmospheres. Moreover, the effect of the addition of SBA-15 and MCM-41 is analyzed. Two different techniques, i.e., TG/FTIR (low heating rates) and EGA Py/GC/MS (high heating rates), are used. At low temperatures NNN is almost unaltered, but it is volatilized and dragged by the carrier gas. When increasing the temperature, decomposition takes place, with pyridines being one of the most abundant compounds observed. The main compound obtained during the pyrolysis are 3- pyridinecarbonitrile, myosmine and nornicotine, which are precursors of NNN. When NNN is mixed with SBA-15, the decomposition of the NNN nitrosamine is favored at low temperatures where the yield in pyridine compounds increases. The catalysts modify the temperature and intensity of the processes, especially under an oxidative atmosphere where the residue is oxidized, showing a third loss of weight. These materials modify the compositions of gases, mainly under an O2 atmosphere (3-pyridinecarbonitrile and myosmine showed the major effect). SBA-15 with fibrous morphology obtains the best reductions at pyrolysis conditions.
Electric Fields Induced in the Brain by Transcranial Electric Stimulation: A Review of In Vivo Recordings
Authors: Guidetti Matteo , Arlotti Mattia , Bocci Tommaso , Bianchi Anna Maria , Parazzini Marta , Ferrucci Roberta , Priori Alberto
Published: September 2022

Transcranial electrical stimulation (tES) techniques, such as direct current stimulation (tDCS) and transcranial alternating current stimulation (tACS), cause neurophysiological and behavioral modifications as responses to the electric field are induced in the brain. Estimations of such electric fields are based mainly on computational studies, and in vivo measurements have been used to expand the current knowledge. Here, we review the current tDCS- and tACS-induced electric fields estimations as they are recorded in humans and non-human primates using intracerebral electrodes. Direct currents and alternating currents were applied with heterogeneous protocols, and the recording procedures were characterized by a tentative methodology. However, for the clinical stimulation protocols, an injected current seems to reach the brain, even at deep structures. The stimulation parameters (e.g., intensity, frequency and phase), the electrodes’ positions and personal anatomy determine whether the intensities might be high enough to affect both neuronal and non-neuronal cell activity, also deep brain structures.
The Oncogenic and Tumor Suppressive Long Non-Coding RNA–microRNA–Messenger RNA Regulatory Axes Identified by Analyzing Multiple Platform Omics Data from Cr(VI)-Transformed Cells and Their Implications in Lung Cancer
Authors: Sweef Osama , Yang Chengfeng , Wang Zhishan
Published: September 2022

Chronic exposure to hexavalent chromium (Cr(VI)) causes lung cancer in humans, however, the underlying mechanism has not been well understood. Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) are commonly studied non-coding RNAs. miRNAs function mainly through interaction with the 3′-untranslated regions of messenger RNAs (mRNAs) to down-regulate gene expression. LncRNAs have been shown to function as competing endogenous RNAs (ceRNAs) to sponge miRNAs and regulate gene expression. It is now well accepted that lncRNAs and miRNAs could function as oncogenes or tumor suppressors. Dysregulations of lncRNAs and miRNAs have been shown to play important roles in cancer initiation, progression, and prognosis. To explore the mechanism of Cr(VI) lung carcinogenesis, we performed lncRNA, mRNA, and miRNA microarray analysis using total RNAs from our previously established chronic Cr(VI) exposure malignantly transformed and passage-matched control human bronchial epithelial BEAS-2B cells. Based on the differentially expressed lncRNAs, miRNAs, and mRNAs between the control (BEAS-2B-Control) and Cr(VI)-transformed (BEAS-Cr(VI)) cells and by using the lncRNA–miRNA interaction and miRNA target prediction algorithms, we identified three oncogenic (HOTAIRM1/miR-182-5p/ERO1A, GOLGA8B/miR-30d-5p/RUNX2, and PDCD6IPP2/miR-23a-3p/HOXA1) and three tumor suppressive (ANXA2P1/miR-20b-5p/FAM241A (C4orf32), MIR99AHG/miR-218-5p/GPM6A, and SH3RF3-AS1/miR-34a-5p/HECW2) lncRNA–miRNA–mRNA regulatory axes. Moreover, the relevance of these three oncogenic and three tumor suppressive lncRNA–miRNA–mRNA regulatory axes in lung cancer was explored by analyzing publicly available human lung cancer omics datasets. It was found that the identified three oncogenic lncRNA–miRNA–mRNA regulatory axes (HOTAIRM1/miR-182-5p/ERO1A, GOLGA8B/miR-30d-5p/RUNX2, and PDCD6IPP2/miR-23a-3p/HOXA1) and the three tumor suppressive lncRNA–miRNA–mRNA regulatory axes (ANXA2P1/miR-20b-5p/FAM241A (C4orf32), MIR99AHG/miR-218-5p/GPM6A, and SH3RF3-AS1/miR-34a-5p/HECW2) have significant diagnostic and prognosis prediction values in human lung cancer. In addition, our recent studies showed that Cr(VI)-transformed cells display cancer stem cell (CSC)-like properties. Further bioinformatics analysis identified the oncogenic lncRNA–miRNA–mRNA regulatory axes as the potential regulators of cancer stemness. In summary, our comprehensive analysis of multiple platform omics datasets obtained from Cr(VI)-transformed human bronchial epithelial cells identified several oncogenic and tumor suppressive lncRNA–miRNA–mRNA regulatory axes, which may play important roles in Cr(VI) carcinogenesis and lung cancer in general.
Effects of the Phenethylamine 2-Cl-4,5-MDMA and the Synthetic Cathinone 3,4-MDPHP in Adolescent Rats: Focus on Sex Differences
Authors: Pisanu Augusta , Lo Russo Giacomo , Talani Giuseppe , Bratzu Jessica , Siddi Carlotta , Sanna Fabrizio , Diana Marco , Porcu Patrizia , De Luca Maria Antonietta , Fattore Liana
Published: September 2022

The illicit drug market of novel psychoactive substances (NPSs) is expanding, becoming an alarming threat due to increasing intoxication cases and insufficient (if any) knowledge of their effects. Phenethylamine 2-chloro-4,5-methylenedioxymethamphetamine (2-Cl-4,5-MDMA) and synthetic cathinone 3,4-methylenedioxy-α-pyrrolidinohexanophenone (3,4-MDPHP) are new, emerging NPSs suggested to be particularly dangerous. This study verified whether these two new drugs (i) possess abuse liability, (ii) alter plasma corticosterone levels, and (iii) interfere with dopaminergic transmission; male and female adolescent rats were included to evaluate potential sex differences in the drug-induced effects. Findings show that the two NPSs are not able to sustain reliable self-administration behavior in rats, with cumulatively earned injections of drugs being not significantly different from cumulatively earned injections of saline in control groups. Yet, at the end of the self-administration training, females (but not males) exhibited higher plasma corticosterone levels after chronic exposure to low levels of 3,4-MDPHP (but not of 2-Cl-4,5-MDMA). Finally, electrophysiological patch-clamp recordings in the rostral ventral tegmental area (rVTA) showed that both drugs are able to increase the firing rate of rVTA dopaminergic neurons in males but not in females, confirming the sex dimorphic effects of these two NPSs. Altogether, this study demonstrates that 3,4-MDPHP and 2-Cl-4,5-MDMA are unlikely to induce dependence in occasional users but can induce other effects at both central and peripheral levels that may significantly differ between males and females.
Immunomodulation by Hemoadsorption—Changes in Hepatic Biotransformation Capacity in Sepsis and Septic Shock: A Prospective Study
Authors: Praxenthaler Janina , Schwier Elke , Altmann Simon , Kirchner Carmen , Bialas Julian , Henzler Dietrich , Köhler Thomas
Published: September 2022

Background: Sepsis is often associated with liver dysfunction, which is an indicator of poor outcomes. Specific diagnostic tools that detect hepatic dysfunction in its early stages are scarce. So far, the immune modulatory effects of hemoadsorption with CytoSorb® on liver function are unclear. Method: We assessed the hepatic function by using the dynamic LiMAx® test and biochemical parameters in 21 patients with sepsis or septic shock receiving CytoSorb® in a prospective, observational study. Points of measurement: T1: diagnosis of sepsis or septic shock; T2 and T3: 24 h and 48 h after the start of CytoSorb®; T4: 24 h after termination of CytoSorb®. Results: The hepatic biotransformation capacity measured by LiMAx® was severely impaired in up to 95 % of patients. Despite a rapid shock reversal under CytoSorb®, a significant improvement in LiMAx® values appeared from T3 to T4. This decline and recovery of liver function were not reflected by common parameters of hepatic metabolism that remained mostly within the normal range. Conclusions: Hepatic dysfunction can effectively and safely be diagnosed with LiMAx® in ventilated ICU patients under CytoSorb®. Various static liver parameters are of limited use since they do not adequately reflect hepatic dysfunction and impaired hepatic metabolism.
Effect of Excessive Screen Time on Cardiorespiratory Fitness in Children: A Longitudinal Study
Authors: Goto Ryo , Isa Tsunenori , Kawaharada Rika , Horibe Kana , Tsuboi Yamato , Nakatsuka Kiyomasa , Uchida Kazuaki , Saeki Kenta , Ono Rei
Published: September 2022

Background: This study investigated the effect of different components of screen time (mobile phone use, TV/video viewing, and video gaming) on cardiorespiratory fitness (CRF) development in children aged 9–12 years. Methods: This was a two-year longitudinal study conducted with 175 children (49.7% girls, mean age = 9.5) in Japan. CRF was assessed using a 20 m shuttle run test conducted at baseline and again at follow-up. Children were categorized as “Good” or “Poor” based on the change in CRF scores for each gender. Screen time was assessed using a self-reported questionnaire at baseline and termed as “high” if children reported ≥ 2 h/day. Univariate and multivariate logistic regression analyses were performed after adjusting for gender, physical activity, and time of data collection. Results: Children scoring “high” on mobile phone use had lower odds of being categorized as “Good” in CRF change (crude odds ratio (OR): 0.34; 95% confidence interval (CI): 0.15–0.90 (adjusted OR: 0.33; 95% CI: 0.12–0.91)). There were no significant effects of TV/video viewing (crude OR: 1.54; 95% CI: 0.84–2.81) and video gaming (crude OR: 0.98; 95% CI: 0.48–1.97) on changes in CRF. Conclusions: Limiting excessive mobile phone usage might be important for ensuring healthy development of CRF in children.
IL-6 Serum Levels Can Enhance the Diagnostic Power of Standard Blood Tests for Acute Appendicitis
Authors: Di Mitri Marco , Parente Giovanni , Bonfiglioli Giulia , Thomas Eduje , Bisanti Cristian , Cordola Chiara , Vastano Marzia , Cravano Sara , Collautti Edoardo , Di Carmine Annalisa , D’Antonio Simone , Gargano Tommaso , Libri Michele , Lima Mario
Published: September 2022

Background: The diagnosis of acute appendicitis (AA) remains challenging, especially in pediatrics, because early symptoms are not specific, and the younger the patient the more difficult their interpretation is. There is a large degree of agreement between pediatric surgeons on the importance of an early diagnosis to avoid complicated acute appendicitis (CAA) and its consequences. The aim of this study is to assess if Interleukin 6 (IL-6) could enhance the sensitivity (Sn) and specificity (Sp) of the currently available and routinely performed diagnostic tools in case of suspected AA in pediatric patients. Materials and Methods: A prospective observational study was conducted including patients who underwent appendectomy between November 2020 and March 2022. We divided patients into three groups: not inflamed appendix (group NA), not complicated AA (group NCAA), and complicated AA (group CAA). We compared the mean values of white blood cells (WBC), neutrophils, fibrinogen, ferritin, aPTT, INR, C-reactive protein (CRP), IL-6, and CRP between the three groups. Then we evaluated Sn, Sp, and odds ratio (OR) of IL-6 and CRP alone and combined. Results: We enrolled 107 patients operated on for AA (22 in Group NA, 63 in Group NCAA, and 21 in group CAA). CRP levels resulted in a significant increase when comparing CAA with NA (p = 0.01) and CAA with NCAA (p = 0.01), whereas no significance was found between NA and NCAA (p = 0.38). A statistically significant increase in average IL-6 levels was found when comparing NCAA with NA (p = 0.04), CAA with NA (p = 0.04), and CAA with NCAA (p = 0.02). Considering CRP alone, its Sn, Sp, and OR in distinguishing NA from AA (both NCAA and CAA together) are 86%, 35%, and 33,17, respectively. Similarly, Sn, Sp, and OR of IL-6 alone are 82%, 54%, and 56, respectively. Combining CRP and IL-6 serum levels together, the Sn increases drastically to 100% with an Sp of 40% and OR of 77. Conclusions: Our study may suggest an important role of IL-6 in the detection of AA in its early stage, especially when coupled with CRP.
Trio-Drug Combination of Sodium Valproate, Baclofen and Thymoquinone Exhibits Synergistic Anticonvulsant Effects in Rats and Neuro-Protective Effects in HEK-293 Cells
Authors: Hyder Pottoo Faheem , Salahuddin Mohammed , Khan Firdos Alam , Albaqshi Batool Taleb , Gomaa Mohamed S. , Abdulla Fatima S. , AlHajri Noora , Alomary Mohammad N.
Published: September 2022

Epilepsy is a chronic brain disorder, with anti-epileptic drugs (AEDs) providing relief from hyper-excitability of neurons, but largely failing to restrain neurodegeneration. We investigated a progressive preclinical trial in rats, whereby the test drugs; sodium valproate (SVP; 150 and 300 mg/kg), baclofen (BFN; 5 and 10 mg/kg), and thymoquinone (THQ; 40 and 80 mg/kg) were administered (i.p, once/day for 15 days) alone, and as low dose combinations, and subsequently tested for antiseizure and neuroprotective potential using electrical stimulation of neurons by Maximal electroshock (MES). The seizure stages were monitored, and hippocampal levels of m-TOR, IL-1β, IL-6 were measured. Hippocampal histopathology was also performed. Invitro and Insilco studies were run to counter-confirm the results from rodent studies. We report the synergistic effect of trio-drug combination; SVP (150 mg/kg), BFN (5 mg/kg) and THQ (40 mg/kg) against generalized seizures. The Insilco results revealed that trio-drug combination binds the Akt active site as a supramolecular complex, which could have served as a delivery system that affects the penetration and the binding to the new target. The potential energy of the ternary complex in the Akt active site after dynamics simulation was found to be −370.426 Kcal/mol, while the supramolecular ternary complex alone was −38.732 Kcal/mol, with a potential energy difference of −331.694 Kcal/mol, which favors the supramolecular ternary complex at Akt active site binding. In addition, the said combination increased cell viability by 267% and reduced morphological changes induced by Pentylenetetrazol (PTZ) in HEK-293 cells, which indicates the neuroprotective property of said combination. To conclude, we are the first to report the anti-convulsant and neuroprotective potential of the trio-drug combination.
Understanding Societal Requirements of CCS Projects: Application of the Societal Embeddedness Level Assessment Methodology in Four National Case Studies
Authors: Mendrinos Dimitrios , Karytsas Spyridon , Polyzou Olympia , Karytsas Constantine , Nordø Åsta Dyrnes , Midttømme Kirsti , Otto Danny , Gross Matthias , Sprenkeling Marit , Peuchen Ruben , Geerdink Tara , Puts Hanneke
Published: September 2022

The DigiMon project aims to develop and demonstrate an affordable, flexible, societally embedded, and smart digital monitoring early warning system for any subsurface CO2 storage field. The societal embeddedness level (SEL) assessment is a novel methodology which provides insight into the societal requirements for technological innovation to be deployed. The SEL assessment framework was applied in four case studies, concerning CCS development in Norway, the Netherlands, Greece, and Germany. The resulting societal embeddedness levels of CCS, on a scale of 1–4, were SEL 3 in Norway with considerable progress towards level 4, followed by the Netherlands with SEL 2 with several initiatives towards offshore demonstration projects, and then by Greece and Germany with SEL 1. The outcomes of the SEL assessments show which societal requirements have been met in current CCS developments and which ones should be improved for CCS deployment. They also show that monitoring currently is a regulatory requirement as part of permitting procedures, while it may alleviate community concerns on safety, provided that it has certain attributes. The insights from the four national case studies are further used in the DigiMon project to develop the innovative societal embedded DigiMon monitoring system.
Embodied Carbon Emissions of the Residential Building Stock in the United States and the Effectiveness of Mitigation Strategies
Authors: Hu Ming
Published: September 2022

According to the 2021 Global Status Report for Buildings and Construction published by the United Nations Environment Programme, global carbon emissions from the building sector in 2019 were nearly 14 gigatons (Gt), representing 38% of total global carbon emissions, including 10% from building construction. In the United States, the largest knowledge gap regarding embodied carbon in buildings exists at the whole-building level. The first step in creating informative policy to reduce embodied carbon emissions is to map the existing building stock emissions and changes over time to understand the primary contributing building types and hot spots (states), and then to compare and analyze mitigation scenarios. To fill this knowledge gap, this study first developed a bottom-up model to assess the embodied carbon of the US residential building stock by using 64 archetypes to represent the building stock. Then, the embodied carbon characteristics of the current building stock were analyzed, revealing that the primary contributor was single-family detached (SD) houses. The results indicated that the exterior wall was a major contributor, and that small multifamily housing was the most embodied carbon-intense building type. Two scenarios, the baseline scenario and progressive scenario, were formed to evaluate the effectiveness of six mitigation strategies. The progressive scenario with all mitigation strategies (M1–M6) applied produced a total reduction of 33.13 Gt CO2eq (42%) in the cumulative residential building stock related to carbon emissions during 2022–2050, and a total reduction of 88.34 Gt CO2eq (80%) during 2022–2100. The results show that with an embodied carbon emissions reduction in the progressive scenario (42% by 2100), the total embodied carbon emissions comply with the carbon budget of a 2 °C pathway, but will exceed the budget for a 1.5 °C pathway.
Eranthis Salisb. (Ranunculaceae) in South Siberia: Insights into Phylogeography and Taxonomy
Authors: Protopopova Marina V. , Pavlichenko Vasiliy V.
Published: September 2022

Eranthis Salisb. (Ranunculaceae) is a herbaceous plant genus, including few species disjunctively distributed throughout the temperate zone from Southeastern Europe to Eastern Asia. Until recently, only Eranthis sibirica DC. was known in South Siberia, being considered endemic and tertiary relict. Not long ago, Eranthis tanhoensis Erst was also described in Siberia. We report here a reconstruction of the phylogenetic relationships between the Siberian Eranthis species based on nuclear (ITS) and plastid (trnL + trnL-trnF + trnH-psbA) DNA. The phylogeographic structure of Siberian Eranthis is distinguished by the presence of the two “eastern” and “western” supergroups, which most likely formed as a result of disjunction caused by active mountain uplifts during the late Neogene–early Quaternary and subsequent progressive Pleistocene cooling. The eastern supergroup combines lineage I, containing populations from the eastern Khamar-Daban Ridge, the Eastern Sayan Mountains, and the Tannu-Ola Ridge, and lineage II containing western Khamar-Daban populations. The western supergroup includes only lineage III, containing Western Sayan populations. Our data clearly show that E. tanhoensis is nested in the E. sibirica clade, thereby indicating that its description as a separate species is unjustified, as it compromises the monophyletic status of E. sibirica. Therefore, we suggest here to consider E. tanhoensis as a synonym of E. sibirica.
Information Environment Quantifiers as Investment Analysis Basis
Authors: Rodionov Dmitry G. , Pashinina Polina A. , Konnikov Evgenii A. , Konnikova Olga A.
Published: September 2022

The combination of the processes of widespread digitalization and globalization of the world economy has led to a significant expansion of the global information environment. The modern information environment is dynamically active, and changes in it are indicators of changes in the material world. This specificity can be used for investment analysis purposes. However, at the time of this research, a universal methodology for analyzing the information environment has not yet been formed. The purpose of this study is to develop tools for quantifying the information environment and testing them as investment predictors. The key result of this study is a stock price forecasting model based on information environment quantifiers and its critical analysis. The results obtained will be useful both for investors of different skill levels and for researchers of the information environment.
Channel Modeling for RIS-Assisted 6G Communications
Authors: Fu Xiuhua , Peng Rongqun , Liu Gang , Wang Jiazheng , Yuan Wenhao , Kadoch Michel
Published: September 2022

Terahertz communication has been proposed as one of the basic key technologies of the sixth-generation wireless network (6G) due to its significant advantages, such as ultra-large bandwidth, ultra-high transmission rates, high-precision positioning, and high-resolution perception. In terahertz-enabled 6G communication systems, the intelligent reconfiguration of wireless propagation environments by deploying reconfigurable intelligent surfaces (RIS) will be an important research direction. This paper analyzes the far field and near field of RIS-assisted wireless communication and a detailed system description is presented. Subsequently, this paper presents a specific study of the channel model for an RIS-assisted 6G communication system in the far-field and near-field cases, respectively. Finally, an integrated simulation of the channel models for the far-field and near-field cases is carried out, and the performance of the RIS auxiliary link measured in terms of signal-to-noise ratio (SNR) is compared and analyzed. The results show that increasing the size of the RIS surface to improve the SNR is an effective method to enhance the coverage performance of the 6G THz communication system under the strong guarantee of the ultra-large bandwidth of THz.
A Sponge-Based Key Expansion Scheme for Modern Block Ciphers
Authors: Sawka Maciej , Niemiec Marcin
Published: September 2022

Many systems in use today require strong cryptographic primitives to ensure confidentiality and integrity of data. This is especially true for energy systems, such as smart grids, as their proper operation is crucial for the existence of a functioning society. Because of this, we observe new developments in the field of cryptography every year. Among the developed primitives, one of the most important and widely used are iterated block ciphers. From AES (Advanced Encryption Standard) to LEA (Lightweight Encryption Algorithm), these ciphers are omnipresent in our world. While security of the encryption process of these ciphers is often meticulously tested and verified, an important part of them is neglected—the key expansion. Many modern ciphers use key expansion algorithms which produce reversible sub-key sequences. This means that, if the attacker finds out a large-enough part of this sequence, he/she will be able to either calculate the rest of the sequence, or even the original key. This could completely compromise the cipher. This is especially concerning due to research done into side-channel attacks, which attempt to leak secret information from memory. In this paper, we propose a novel scheme which can be used to create key expansion algorithms for modern ciphers. We define two important properties that a sequence produced by such algorithm should have and ensure that our construction fulfills them, based on the research on hashing functions. In order to explain the scheme, we describe an example algorithm constructed this way, as well as a cipher called IJON which utilizes it. In addition to this, we provide results of statistical tests which show the unpredictability of the sub-key sequence produced this way. The tests were performed using a test suite standardized by NIST (National Institute for Standards and Technology). The methodology of our tests is also explained. Finally, the reference implementation of the IJON cipher is published, ready to be used in software. Based on the results of tests, we conclude that, while more research and more testing of the algorithm is advised, the proposed key expansion scheme provides a very good generation of unpredictable bits and could possibly be used in practice.
Phase Change Slurries for Cooling and Storage: An Overview of Research Trends and Gaps
Authors: Borri Emiliano , Hua Nan , Sciacovelli Adriano , Wu Dawei , Ding Yulong , Li Yongliang , Brancato Vincenza , Zhang Yannan , Frazzica Andrea , Li Wenguang , Yu Zhibin , Milian Yanio E. , Ushak Svetlana , Grageda Mario , Cabeza Luisa F.
Published: September 2022

Phase change slurries (PCSs) have great potential as both a heat transfer fluid and an energy storage medium for cooling processes, cold energy storage, and cold energy transportation due to desirable thermophysical properties. One of the major benefits of PCSs compared to pure phase change materials is their fluidity, thus making them cooled or heated by a heat exchanger, pumped through pipes, discharged, and stored directly in a thermal energy storage tank. The use of encapsulated phase change slurries and gas hydrate slurry has thus attracted considerable interest as reflected in the literature with a rising number of publications and institutions involved in the area. The use of bibliometric techniques has found a recent interest in the literature to define the progress of different scientific topics and inspire researchers to identify novelties. In this paper, bibliometric analysis and a detailed systematic review are carried out to show the state-of-the-art development of PCSs for cooling applications. Research gaps and hotspots are identified to help define future perspectives on this topic.
Quantitative Power Flow Characterization of Energy Harvesting Shock Absorbers by Considering Motion Bifurcation
Authors: Li Jing , Wang Peiben , Gao Yuewen , Guan Dong , Li Shengquan
Published: September 2022

Vibration energy harvesting technology can capture ambient energy forms. Using an energy harvesting shock absorber (EHSA) is one of the methods to achieve this function. The EHSA with mechanical motion rectifier (MMR) has motion bifurcation, which can improve energy harvesting performance and reduce the impact between gears. However, the motion bifurcation makes it difficult to quantitatively predict the vibrational energy dissipation and energy harvesting of the MMR−EHSA. Evaluating the performance of an MMR−EHSA during the design phase becomes highly complex. In this paper, a novel nonlinear dynamics model of MMR−EHSAs is established to solve motion bifurcation and quantitative power flow. Furthermore, the proposed MMR−EHSA prototype is fabricated, and dynamics testing is initiated to verify the theoretical model under harmonic vibration. The testing results show that the theoretical model can predict the working characterization of MMR−EHSAs. The resistance of optimal harvesting energy and maximum damping power is revealed by the quantitative power flow model under harmonic vibration. In addition, the working performance under random vibration is discussed. The proposed nonlinear dynamics model has advantages when solving random vibration input and has potential for practical application.