bluuebunny/crossref_metadata_2025 huggingface dataset:
NUF2 overexpression contributes to epithelial ovarian cancer progression via ERBB3-mediated PI3K-AKT and MAPK signaling axes
Authors: Leng Ruobing , Meng Yunfang , Sun Xiaomei , Zhao Yingzi
Published: December 2022

Introduction

NDC80 kinetochore complex component (NUF2) is upregulated and plays an important role in various human cancers. However, the function and mechanism of NUF2 in epithelial ovarian cancer (EOC) remain unclear.

Methods

NUF2 expression was detected in EOC tissues and cell lines. The effects of NUF2 downregulation on cell proliferation, migration and invasion in EOC were analyzed by CCK-8 and Transwell assays. Meanwhile, the effect of NUF2 downregulation on tumor growth in vivo was determined by xenograft tumor models. The mechanisms by which NUF2 regulates EOC progression were detected by RNA sequencing and a series of in vitro assays.

Results

We showed that NUF2 was significantly upregulated in EOC tissues and cell lines, and high NUF2 expression was associated with FIGO stage, pathological grade and poor EOC prognosis. NUF2 downregulation decreased cell proliferation, migration, invasion and tumor growth in nude mice. RNA sequencing studies showed that NUF2 knockdown inhibited several genes enriched in the phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)-AKT serine/threonine kinase (AKT) and mitogen-activated protein kinase (MAPK) signaling pathways. Erb-B2 receptor tyrosine kinase 3 (ERBB3) was the key factor involved in both of the above pathways. We found that ERBB3 silencing could inhibit EOC progression and repress activation of the PI3K-AKT and MAPK signaling pathways. Furthermore, the exogenous overexpression of ERBB3 partially reversed the inhibitory effects on EOC progression induced by NUF2 downregulation, while LY294002 and PD98059 partially reversed the effects of ERBB3 upregulation.

Conclusion

These results showed that NUF2 promotes EOC progression through ERBB3-induced activation of the PI3K-AKT and MAPK signaling axes. These findings suggest that NUF2 might be a potential therapeutic target for EOC.
Liver-related long-term outcomes of alpha-glucosidase inhibitors in patients with diabetes and liver cirrhosis
Authors: Yen Fu-Shun , Hou Ming-Chih , Wei James Cheng-Chung , Shih Ying-Hsiu , Hsu Chung Y. , Hsu Chih-Cheng , Hwu Chii-Min
Published: December 2022

Background: Adequate management of diabetes in patients with liver cirrhosis can be challenging. We conducted this study to investigate the liver-related long term outcomes of alpha-glucosidase inhibitors (AGIs) in patients with diabetes and cirrhosis.

Methods: From National Health Insurance Research Database (NHIRD) in Taiwan, we recruited propensity-score matched alpha-glucosidase inhibitor users and non-users from a cohort of type 2 diabetes mellitus (T2DM) with compensated liver cirrhosis between 1 January 2000, and 31 December 2017, and followed them until 31 December 2018. Cox proportional hazards models with robust sandwich standard error estimates were used to assess the risk of main outcomes for alpha-glucosidase inhibitor users versus non-users.

Results: The incidence rates of mortality during follow-up were 65.56 vs. 96.06 per 1,000 patient-years for alpha-glucosidase inhibitor users and non-users, respectively. The multivariable-adjusted model shows that alpha-glucosidase inhibitor users had significantly lower risks of all-cause mortality (aHR 0.63, 95% CI 0.56–0.71), hepatocellular carcinoma (aHR 0.55, 95% CI 0.46–0.67), decompensated cirrhosis (aHR 0.74 95% CI 0.63–0.87), hepatic encephalopathy (aHR 0.72, 95% CI 0.60–0.87), and hepatic failure (aHR 0.74, 95% CI 0.62–0.88) than alpha-glucosidase inhibitor non-users. Patients who received alpha-glucosidase inhibitors for a cumulative duration of more than 364 days had significantly lower risks of these outcomes than non-users.

Conclusion: Alpha-glucosidase inhibitor use was associated with a lower risk of mortality, hepatocellular carcinoma, decompensated cirrhosis, and hepatic failure in patients with diabetes and compensated cirrhosis. alpha-glucosidase inhibitors may be useful for the management of diabetes in patients with compensated liver cirrhosis. Large-scale prospective studies are required to verify our results.
Clinical efficacy of azacytidine and venetoclax and prognostic impact of Tim-3 and galectin-9 in acute myeloid leukemia and high-risk myelodysplastic syndromes: A single-center real-life experience
Authors: Giudice Valentina , Serio Bianca , Ferrara Idalucia , Manzo Paola , Gorrese Marisa , Pepe Rita , Bertolini Angela , D’Alto Francesca , Verdesca Francesco , Langella Maddalena , Filippelli Amelia , Selleri Carmine
Published: December 2022

Treatment of acute myeloid leukemia (AML) and high-risk myelodysplastic syndromes (MDS) is difficult in older patients with comorbidities and high-risk disease factors. Venetoclax, the first-in-class Bcl-2 inhibitor, has proven efficacy and safety in combination with azacytidine for treatment of high-risk myeloid diseases. In this single-center real-life retrospective study, a total of 27 consecutive patients treated with azacytidine plus venetoclax were included, and clinical outcomes, hematological improvements, and biomarkers of responsiveness to therapy were compared to those observed in an historical cohort of 95 consecutive patients treated with azacytidine as single agent. Azacytidine plus venetoclax was effective and safe in older and frail AML and high-risk MDS patients, with median overall survival of 22.3 months, higher than that reported in phase III trial (14.7 months), and higher than that of historical cohort (5.94 months). Progression-free survival was higher in patients treated with the drug combination compared to those treated with azacytidine as single agent (p = 0.0065). Clinical benefits might increase when azacytidine and venetoclax are administered as upfront therapy (p = 0.0500). We showed that Tim-3 expression could be a promising therapeutic target in refractory/relapsed patients, and galectin-9 a biomarker of responsiveness to therapy. Moreover, patients treated with azacytidine and venetoclax displayed a higher overall survival regardless the presence of negative prognostic markers at diagnosis (e.g., increased WT1 copies and/or normalized blast count). These encouraging results in a real-world setting supported efficacy and safety of azacytidine plus venetoclax as upfront therapy in AML and high-risk MDS, with clinical outcomes comparable to those of clinical trials when an appropriate venetoclax management with bone marrow assessment at every first, second, fourth, and eighth cycle, and dose adjustments for toxicities are performed.
Integrated transcriptomic and metabolomic analyses of the molecular mechanisms of two highland barley genotypes with pyroxsulam responses
Authors: Weng Hua , Yan Jiahui , Guo Liangzhi , Chen Hongyu
Published: December 2022

Highland barley is one of the few crops that can be grown at high elevations, making it a key resource within the Tibet Plateau. Weeds are a significant threat to highland barley production, and new herbicides and tolerant barley varieties are needed to control this ever-growing problem. A better understanding of existing herbicide resistance mechanisms is therefore needed. In this study, transcriptomic and metabolomic analyses were used to identify molecular and physiological changes in two highland barley genotypes with differing sensitivities to the herbicide pyroxsulam. We identified several stress-responsive metabolites, including flavonoids and antioxidants, which accumulated to significantly higher levels in the pyroxsulam-resistant genotype. Additionally, we found key genes in both the flavonoid biosynthesis pathway and the antioxidant system that were up-regulated in pyroxsulam-resistant barley. This work significantly expands on the current understanding of the molecular mechanisms underlying differing pyroxsulam tolerance among barley genotypes and provides several new avenues to explore for breeding or engineering tolerant barley.
Responses of the bacterial community of tobacco phyllosphere to summer climate and wildfire disease
Authors: Wang Zhenhua , Fu Changwu , Tian Jinyan , Wang Wei , Peng Deyuan , Dai Xi , Tian Hui , Zhou Xiangping , Li Liangzhi , Yin Huaqun
Published: December 2022

Both biotic and abiotic factors continually affect the phyllospheric ecology of plants. A better understanding of the drivers of phyllospheric community structure and multitrophic interactions is vital for developing plant protection strategies. In this study, 16S rRNA high-throughput sequencing was applied to study how summer climatic factors and bacterial wildfire disease have affected the composition and assembly of the bacterial community of tobacco (Nicotiana tabacum L.) phyllosphere. Our results indicated that three time series groups (T1, T2 and T3) formed significantly distinct clusters. The neutral community model (NCM) and beta nearest taxon index (betaNTI) demonstrated that the overall bacterial community assembly was predominantly driven by stochastic processes. Variance partitioning analysis (VPA) further showed that the complete set of the morbidity and climatic variables together could explain 35.7% of the variation of bacterial communities. The node numbers of the molecular ecological networks (MENs) showed an overall uptrend from T1 to T3. Besides, Pseudomonas is the keystone taxa in the MENs from T1 to T3. PICRUSt2 predictions revealed significantly more abundant genes of osmoprotectant biosynthesis/transport in T2, and more genes for pathogenicity and metabolizing organic substrate in T3. Together, this study provides insights into spatiotemporal patterns, processes and response mechanisms underlying the phyllospheric bacterial community.
CARK3-mediated ADF4 regulates hypocotyl elongation and soil drought stress in Arabidopsis
Authors: Peng Lu , He Juan , Yao Huan , Yu Qin , Zhang Qian , Li Kexuan , Huang Yaling , Chen Li , Li Xufeng , Yang Yi , Li Xiaoyi
Published: December 2022

Actin depolymerization factors (ADFs), as actin-binding proteins, act a crucial role in plant development and growth, as well as in response to abiotic and biotic stresses. Here, we found that CARK3 plays a role in regulating hypocotyl development and links a cross-talk between actin filament and drought stress through interaction with ADF4. By using bimolecular fluorescence complementation (BiFC) and GST pull-down, we confirmed that CARK3 interacts with ADF4 in vivo and in vitro. Next, we generated and characterized double mutant adf4cark3-4 and OE-ADF4:cark3-4. The hypocotyl elongation assay indicated that the cark3-4 mutant seedlings were slightly longer hypocotyls when compared with the wild type plants (WT), while CARK3 overexpressing seedlings had no difference with WT. In addition, overexpression of ADF4 significantly inhibited long hypocotyls of cark3-4 mutants. Surprisingly, we found that overexpression of ADF4 markedly enhance drought resistance in soil when compared with WT. On the other hand, drought tolerance analysis showed that overexpression of CARK3 could rescue adf4 drought susceptibility. Taken together, our results suggest that CARK3 acts as a regulator in hypocotyl elongation and drought tolerance likely via regulating ADF4 phosphorylation.
The genome wide analysis of Tryptophan Aminotransferase Related gene family, and their relationship with related agronomic traits in Brassica napus
Authors: Cheng Xin , Liu Xinmin , He Jianjie , Tang Mi , Li Huaixin , Li Maoteng
Published: December 2022

Tryptophan Aminotransferase of Arabidopsis1/Tryptophan Aminotransferase-Related (TAA1/TAR) proteins are the enzymes that involved in auxin biosynthesis pathway. The TAA1/TAR gene family has been systematically characterized in several plants but has not been well reported in Brassica napus. In the present study, a total of 102 BnTAR genes with different number of introns were identified. It was revealed that these genes are distributed unevenly and occurred as clusters on different chromosomes except for A4, A5, A10 and C4 in B. napus. Most of the these BnTAR genes are conserved despite of existing of gene loss and gene gain. In addition, the segmental replication and whole-genome replication events were both play an important role in the BnTAR gene family formation. Expression profiles analysis indicated that the expression of BnTAR gene showed two patterns, part of them were mainly expressed in roots, stems and leaves of vegetative organs, and the others were mainly expressed in flowers and seeds of reproductive organs. Further analysis showed that many of BnTAR genes were located in QTL intervals of oil content or seed weight, for example BnAMI10 was located in cqOC-C5-4 and cqSW-A2-2, it indicated that some of the BnTAR genes might have relationship with these two characteristics. This study provides a multidimensional analysis of the TAA1/TAR gene family and a new insight into its biological function in B. napus.
The sporting resilience model: A systematic review of resilience in sport performers
Authors: Gupta Sahen , McCarthy Paul Joseph
Published: December 2022

We systematically reviewed resilience research in sport and exercise psychology. Sample included 92 studies comprising empirical qualitative and quantitative studies, mixed-method studies, review studies and conceptual/theoretical studies on psychological resilience in sports context. From the findings, we synthesized an evidence-based sport-specific definition and meta-model of “Sporting Resilience.” The review incorporates evidence from global culture contexts and evidence synthesized into the new definition and meta-model to achieve its aim. Conceptual detail and testability of the operational definition is provided. Sporting resilience provides a guiding framework for research and applied practice in a testable, objective manner. The new theoretical meta-model of resilience is derived from systematic evidence from sport psychology with theoretical considerations from positive and clinical psychology allowing generalizability. This original theory posits that there is a resilience filter comprised of biopsychosocial protective factors. The strength of this filter determines the impact of adversity and establishes the trajectory of positive adaptation. The findings of the review are used to discuss potential avenues of future research for psychological resilience in sports psychology.

Systematic review registration

https://doi.org/10.17605/OSF.IO/AFWRU.
Empowering leadership and job satisfaction of academic staff in Palestinian universities: Implications of leader-member exchange and trust in leader
Authors: Horoub Ibrahim , Zargar Pouya
Published: December 2022

Introduction

In the aftermath of global pandemic of COVID-19, many sectors faced severe challenges to maintain security, health (psychological, and physical), and steer through the crisis by sup-porting the society.

Methods

Through a quantitative approach a total of 250 surveys were distributed after a pilot test. Specifically, this research gathers data from 178 (71.2% response rate) university teachers from different universities across Palestine via surveys that address the role of empowering leaders on job satisfaction among teachers. The proposed model of the re-search was analyzed using Smart-PLS and PLS-SEM technique.

Discussion and Results

The academic sector was disrupted and faced extreme changes during the pandemic, rendering teachers vulnerable and thus, role of leaders more crucial. Building on job demand-resources model, and social exchange theory, the current study examines the moderating effect of leader-member exchange (LMX) for increasing job satisfaction that can lead to enhanced overall wellbeing in the academic setting. Additionally, the mediating role of trust in leader is focused as a vital psychological element. While the results show a significantly positive effect on job satisfaction in the presence of empowering leaders, the moderating role of LMX alongside mediating impact of trust are observed. This implies that empowering leaders are highly influential in enhancing workplace for university teachers in the post-pandemic era.
Emptiness in the study of emotions in the teaching-learning process of reading-writing during the COVID-19 pandemic
Authors: Martí-González Mariacarla , Barrasa Angel , Belli Simone , Espinel Johana , Da Costa Silvia , López-Granero Caridad
Published: December 2022

The teaching-learning process of reading and writing has great relevance in the psycho-emotional and socio-psychological development of school-age children. This is an exercise in which they develop imagination, attention and memory capacity and through this process the management of emotions and sensitivity and capacity of apprehension of reality. The crisis derived from the COVID-19 pandemic transformed reality in an unprecedented way in the recent history of humanity and the educational context was shaken by all these changes. With confinement, the teaching-learning process of reading and writing, which is designed to take place in person, had to be developed in a hybrid or online way, which was a major challenge for teachers and families and, of course, also for children who were in the process of learning. One of the aspects that was blurred in this context was the role of the teacher, which in this case is one of the most important elements, to achieve adequate learning of reading and writing. On one hand, the teacher is in charge of helping to manage the emotions derived from the learning process itself and, on the other hand, he is a key figure so that this is not only technical learning but also contributes to the child’s socio-emotional development. The aim of this study was to analyze the impact of the socio-psychological changes that have occurred in the educational context since the COVID-19 pandemic on the emotions linked to the teaching process, learning of reading and writing through a systematic review of the studies carried out on the subject, in order to provide recommendations for face-to-face learning in the post-COVID-19 era. A systematic review strategy was devised and the literature search was conducted. The search was conducted using ERIC, Dialnet, Scopus, WoS, EBSCO, and Google Scholar databases. This systematic review took place during the month of May 2022. The results show that given the scarce presence of empirical studies on the subject, the objective has only been partially met. However, a systematic review of the studies carried out on the subject. For the identification of recommendations in the development of face-to-face learning in the post-covid era, it has been possible to identify some ideas of interest for future curricular designs in primary school students who are immersed in learning to read and write.
Benzodiazepines in sport, an underestimated problem: Recommendations for sports medicine physicians’ practice
Authors: Zandonai Thomas , Peiró Ana María , Fusina Francesca , Lugoboni Fabio , Zamboni Lorenzo
Published: December 2022

In the last years, only few studies in literature have focused on the use and abuse of benzodiazepines (BZDs) in sport. Benzodiazepine-related problems include misuse, addiction, driving impairments, and morbidity and mortality related to overdose and withdrawal. Two clinical cases regarding elite endurance athletes evidenced that they had started to use BZDs to counteract insomnia, to recover faster from training sessions and to manage muscle pain. One of the important points that emerged from their stories was that their sports doctors did not recognize the drugs’ addictive properties, and did not intervene to gradually reduce the dosage. Experts have previously provided recommendations for BZD therapy management in clinical practice. In this article, we would like to address sports medicine physicians specifically and provide guidelines to help them manage situations involving BZD prescription, the recognition of addiction, and intervention strategies.
Sense of happiness and other aspects of quality of life in patients with obsessive-compulsive disorder
Authors: Żerdziński Maciej , Burdzik Marcin , Żmuda Roksana , Witkowska-Berek Agnieszka , Dȩbski Paweł , Flajszok-Macierzyńska Natalia , Piegza Magdalena , John-Ziaja Hanna , Gorczyca Piotr
Published: December 2022

Introduction

Quality of life (QoL) is the intellectual and emotional wellbeing of an individual, which is determined by many factors. The most measurable are the sense of happiness, occupational satisfaction, quality of interpersonal relationships and sex life. Obsessive-compulsive disorder (OCD) is a chronic mental disorder diagnosed by the presence of obsessions and compulsions that disrupt normal psychosocial functioning. Despite early onset, treatment is delayed (OCD-DTI) and unsatisfactory.

Objectives

The primary objective of this study is to assess selected correlates of the sense of happiness among patients with OCD. The secondary objective is to compare the sense of happiness with healthy people.

Methods

Seventyfive OCD patients and equal number of healthy subjects were compared using a Polish adaptation of the Oxford Happiness Questionnaire (OHQ-23).

Results

We found a significant negative correlation between sense of happiness and the severity of OCD (r = −0.479), the number of obsessive-compulsive personality traits (r = −0.323), the number of other comorbid mental disorders (r = −0.272), the level of aggression (r = −0.448), impulsivity (r = −0.301), depressiveness (r = −0.357), and the sexual dysfunctions (r = −0.279). The latter were much more common in individuals with OCD compared to healthy subjects (66.67 vs. 12%). The level of loneliness was over two times higher in the study group compared to controls (27 vs. 12%). The mean delay in treatment onset was 13 years. Conclusions. Assessment of aspects of QoL should be an integral part of the diagnostic and therapeutic process in OCD.
Waterborne diseases and ethnic-related disparities: A 10 years nationwide mortality and burden of disease analysis from Ecuador
Authors: Ortiz-Prado Esteban , Simbaña-Rivera Katherine , Cevallos Gabriel , Gómez-Barreno Lenin , Cevallos Domenica , Lister Alex , Fernandez-Naranjo Raul , Ríos-Touma Blanca , Vásconez-González Jorge , Izquierdo-Condoy Juan S.
Published: December 2022

Background

Despite worldwide progress in terms of clean water supply, sanitation, and hygiene knowledge, some middle and most of low-income countries are still experiencing many diseases transmitted using unsafe water and the lack of sanitation.

Methods

To understand the impact of all waterborne diseases (WBD) registered in Ecuador. We performed a population-based analysis of all cases and deaths due to WBD in Ecuador based on the national public databases of hospital discharges as a proxy of incidence, in-hospital mortality, and countrywide general mortality rates from 2011 to 2020.

Results

In Ecuador, mestizos (mixed European and Indigenous American ancestry) had the greatest morbidity rate (141/100,000), followed by indigenous (63/100,000) and self-determined white patients (21/100,000). However, in terms of mortality, indigenous population have the greatest risk and rates, having a 790% additional mortality rate (2.6/100,000) than the reference group (self-determined white populations) at 0.29/100,000. The burden of disease analysis demonstrated that indigenous had the highest burden of disease caused by WBD with 964 YLL per every 100,000 people while mestizos have 360 YYL per 100,000 and self-determined white Ecuadorians have 109 YYL per 100,000.

Conclusions

In Ecuador, waterborne diseases (WBD) are still a major public health problem. We found that indigenous population had higher probability of getting sick and die due to WBD than the rest of the ethnic groups in Ecuador. We also found that younger children and the elderly are more likely to be admitted to the hospital due to a WBD. These epidemiological trends are probably associated with the lower life expectancy found among Indigenous than among the rest of the ethnic groups, who die at least, 39 years earlier than the self-determined white populations, 28 years earlier than Afro-Ecuadorians and 12 years earlier than the mestizos.
How fast and how well the Omicron epidemic was curtailed. A Guangzhou experience to share
Authors: Cai Wenfeng , Yang Zifeng , Liang Jingyi , Lin Zhengshi , Ma Yu , Chen Chun , Li Yan , Li Yongming , Mai Zhitong , Mai Kailin , Kong Xuetao , Liang Xingyi , Li Qianying , Tu Chuanmeizi , Chen Canxiong , Hon Chitin , Qin Pengzhe , Li Ke , Li Xiaoning , Miao Yutian , Liu Xuexing , Guan Wenda , Zeng Zhiqi , Qiu Wanli , He Wei , Zhang Lin , Yang Zhicong , Zhong Nanshan
Published: December 2022

Introduction

SARS-CoV-2 has ravaged the world and undergone multiple mutations during the course of the COVID-19 pandemic. On 7 April 2022, an epidemic caused by SARS-CoV-2 Omicron (BA.2) variant broke out in Guangzhou, China, one of the largest transportation and logistical hubs of the country.

Methods

To fast curtained the Omicron epidemic, based on the routine surveillance on the risk population of SARS-CoV-2 infection, we identify key places of the epidemic and implement enhanced control measures against Omicron.

Results

Transmission characteristics of the Omicron variant were analyzed for 273 confirmed cases, and key places involved in this epidemic were fully presented. The median incubation time and the generation time were 3 days, and the reproduction number Rt was sharply increased with a peak of 4.20 within 2 days. We tried an all-out effort to tackle the epidemic in key places, and the proportion of confirmed cases increased from 61.17% at Stage 2 to 88.89% at Stage 4. Through delimited risk area management, 99 cases were found, and the cases were isolated in advance for 2.61 ± 2.76 days in a lockdown zone, 0.44 ± 1.08 days in a controlled zone, and 0.27 ± 0.62 days in a precautionary zone. People assigned with yellow code accounted for 30.32% (84/277) of confirmed COVID-19 cases, and 83.33% of them were detected positive over 3 days since code assignment. For the districts outside the epicenter, the implementation duration of NPIs was much shorter compared with the Delta epidemic last year.

Conclusion

By blocking out transmission risks and adjusting measures to local epidemic conditions through the all-out effort to tackle the epidemic in key places, by delimiting risk area management, and by conducting health code management of the at-risk population, the Omicron epidemic could be contained quickly.
Framework for qualifying exoskeletons as adaptive support technology
Authors: Ott Oliver , Ralfs Lennart , Weidner Robert
Published: December 2022

The fifth industrial revolution and the accompanying influences of digitalization are presenting enterprises with significant challenges. Regardless of the trend, however, humans will remain a central resource in future factories and will continue to be required to perform manual tasks. Against the backdrop of, e.g., societal and demographic changes and skills shortage, future-oriented support technologies such as exoskeletons represent a promising opportunity to support workers. Accordingly, the increasing interconnection of human operators, devices, and the environment, especially in human-centered work processes, requires improved human-machine interaction and further qualification of support systems to smart devices. In order to meet these requirements and enable exoskeletons as a future-proof technology, this article presents a framework for the future-oriented qualification of exoskeletons, which reveals potential in terms of user-individual and context-dependent adaptivity of support systems. In this context, a framework has been developed, allowing different support situations to be classified based on elementary functions. Using these support function dependencies and characteristics, it becomes possible to describe adaptive system behavior for human-centered support systems such as exoskeletons as a central aspect. For practical illustration, it is shown for an exemplary active exoskeleton using the example of user-individuality and context-specificity how the support characteristics of exoskeletons in the form of different support characteristics can bring about a purposeful and needs-based application for users and can contribute valuably to design future workplaces.
Transanal hemorrhoidal dearterialization (THD) for hemorrhoidal disease: An Italian single-institution 5-year experience analysis and updated literature review
Authors: Verre Luigi , Gallo Gaetano , Grassi Giulia , Bussolin Edoardo , Carbone Ludovico , Poto Gianmario Edoardo , Carpineto Samorani Osvaldo , Marano Luigi , Marrelli Daniele , Roviello Franco
Published: December 2022

Background

Hemorrhoidal disease is a highly prevalent, chronic disorder that usually compromise patients' quality of life. Despite recent advances in pharmacologic and surgical therapeutic options, a clear treatment “gold standard” is lacking. Our aim is to analyze the outcomes following Transanal Hemorrhoidal Dearterialization (THD) procedure.

Methods

Patients who failed conservative treatment and underwent THD Doppler between 2017 and 2021 were enrolled. Follow-up interviews (consisting of clinical examination, Visual Analog Scale for pain—VAS, Vaizey incontinence score, Hemorrhoid Severity Score) were administered 1 week, 2 weeks, 1 month and 6 months after surgery.

Results

Forty-seven out of 75 patients were male, and the mean age was 50 (± 17.9) years. Hemorrhoids were classified as Goligher's degree II in 25 cases, III in 40 and IV, simple irreducible without ischemic changes, in 10. The mean operative time was 35 (28–60) minutes, and most procedures were performed with epidural anesthesia (80%). No intraoperative complications occurred, and 73 patients (97.3%) were discharged within post-operative day 1. Early post-operative pain and bleeding occurred in 37.3% and 8% of patients, respectively. No patients experienced anal incontinence and severe symptoms at 6 months after surgery. The overall success rate was 97.3%.

Conclusions

THD is safe and effective in hemorrhoidal disease at degree II if bleeding, III, and IV without ischemic changes, both as a first intervention and on recurrence. Physician and patient need to understand each other's expectations, weight the risks and benefits, and customize the treatment.
Feed intake, emission of enteric methane and estimates, feed efficiency, and ingestive behavior in buffaloes supplemented with palm kernel cake in the Amazon biome
Authors: Amaral Júnior João Maria do , Martorano Lucieta Guerreiro , Nahúm Benjamim de Souza , de Castro Vinícius Costa Gomes , Sousa Luciano Fernandes , Rodrigues Thomaz Cyro Guimarães de Carvalho , da Silva Jamile Andréa Rodrigues , da Costa Silva Artur Luiz , Lourenço Júnior José de Brito , Berndt Alexandre , e Silva André Guimarães Maciele
Published: December 2022

The use of palm kernel cake as an alternative to conventional ingredients, due to the presence of residual fat, can also reduce methane emissions. The objective of the study was to evaluate, in two different experiments, the effects of palm kernel cake supplementation on feed intake, enteric methane production and estimates, and the ingestive behavior of buffaloes in the Amazon biome. In experiment 1, to evaluate feed intake, methane production, and feed efficiency, 20 crossbred females, dry and empty, with a mean age of 34 months and an initial body weight of 514 ± 69 kg, were supplemented with palm kernel cake for 60 days. The supply was calculated in relation to body weight (BW) in four treatments: 0% (control); 0.25, 0.50, and 1% of palm kernel cake, distributed in a completely randomized design. In experiment 2, to evaluate the ingestive behavior, 24 mixed-breed, dry, and non-pregnant buffaloes supplemented with palm kernel cake were evaluated in the less rainy season (LR) and the wettest season (WS) of the eastern Amazon, distributed in a completely randomized in the same treatments as experiment 1. The inclusion of palm kernel cake in the supplementation increased the feed intake of dry matter and components (MM, OM, CP, NDF, ADF, and EE) (P < 0.01), reducing the production of enteric methane intake (P < 0.01), the ratio per kg of meat produced (P < 0.01) and feed efficiency (P < 0.01), and influenced the ingestive behavior (time grazing, rumination, and idleness) during the day. We suggest that further research be carried out to verify the results and improve the use of this co-product as a methanogenesis mitigator.
Assessing Population Structure and Signatures of Selection in Wanbei Pigs Using Whole Genome Resequencing Data
Authors: Zhang Wei , Liu Linqing , Zhou Mei , Su Shiguang , Dong Lin , Meng Xinxin , Li Xueting , Wang Chonglong
Published: December 2022

Wanbei pig (WBP) is one of the indigenous pig resources in China and has many germplasm characteristics. However, research on its genome is lacking. To assess the genomic variation, population structure, and selection signatures, we resequenced 18 WBP for the first time and performed a comprehensive analysis with resequenced data of 10 Asian wild boars. In total, 590.03 Gb of data and approximately 41 million variants were obtained. Polymorphism level (θπ) ratio and genetic differentiation (fixation index)-based cross approaches were applied, and 539 regions, which harbored 176 genes, were selected. Functional analysis of the selected genes revealed that they were associated with lipid metabolism (SCP2, APOA1, APOA4, APOC3, CD36, BCL6, ADCY8), backfat thickness (PLAG1, CACNA2D1), muscle (MYOG), and reproduction (CABS1). Overall, our results provide a valuable resource for characterizing the uniqueness of WBP and a basis for future breeding.
Energy Assessment from Broiler Chicks’ Vocalization Might Help Improve Welfare and Production
Authors: Pereira Erica , Nääs Irenilza de Alencar , Ivale André Henrique , Garcia Rodrigo Garófallo , Lima Nilsa Duarte da Silva , Pereira Danilo Florentino
Published: December 2022

Vocalization seems to be a viable source of signal for assessing broiler welfare. However, it may require an understanding of the birds’ signals, both quantitatively and qualitatively. The delivery of calls with a specific set of acoustic features must be understood to assess the broiler’s well-being. The present study aimed to analyze broiler chick vocalization through the sounds emitted during social isolation and understand what would be the flock size where the chicks present the smallest energy loss in vocalizing. The experiments were carried out during the first 3 days of growth, and during the trial, chicks received feed and water ad libitum. A total of 30 1-day-old chicks Cobb® breed were acquired at a commercial hatching unit. The birds were tested from 1 to 3 days old. A semi-anechoic chamber was used to record the vocalization with a unidirectional microphone connected to a digital recorder. We placed a group of 15 randomly chosen chicks inside the chamber and recorded the peeping sound, and the assessment was conducted four times with randomly chosen birds. We recorded the vocalization for 2 min and removed the birds sequentially stepwise until only one bird was left inside the semi-anechoic chamber. Each audio signal recorded during the 40 s was chosen randomly for signal extraction and analysis. Fast Fourier transform (FFT) was used to extract the acoustic features and the energy emitted during the vocalization. Using data mining, we compared three classification models to predict the rearing condition (classes distress and normal). The results show that birds’ vocalization differed when isolated and in a group. Results also indicate that the energy spent in vocalizing varies depending on the size of the flock. When isolated, the chicks emit a high-intensity sound, “alarm call”, which uses high energy. In contrast, they spent less energy when flocked in a group, indicating good well-being when the flock was 15 chicks. The weight of birds influenced the amount of signal energy. We also found that the most effective classifier model was the Random Forest, with an accuracy of 85.71%, kappa of 0.73, and cross-entropy of 0.2.
Isolation and Identification of Bone Marrow Mesenchymal Stem Cells from Forest Musk Deer
Authors: Luo Weiqiang , Geng Yangyang , Gao Mengxi , Cao Mengting , Wang Junjian , Yang Jing , Sun Chenxuan , Yan Xingrong
Published: December 2022

The forest musk deer (Moschus berezovskii) is an endangered animal that produces musk that is utilized for medical applications worldwide, and this species primarily lives in China. Animal-derived musk can be employed as an important ingredient in Chinese medicine. To investigate the properties of bone marrow mesenchymal stem cells (MSCs) obtained from the bone marrow of forest deer for future application, MSCs were isolated and cultivated in vitro. The properties and differentiation of these cells were assessed at the cellular and gene levels. The results show that 81,533 expressed genes were detected by RNA sequencing, and marker genes of MSCs were expressed in the cells. Karyotype analysis of the cells determined the karyotype to be normal, and marker proteins of MSCs were observed to be expressed in the cell membranes. Cells were differentiated into osteoblasts, adipocytes, and chondroblasts. The expression of genes related to osteoblasts, adipocytes, and chondroblasts was observed to be increased. The results of this study demonstrate that the properties of the cells isolated from bone marrow were in keeping with the characteristics of MSCs, providing a possible basis for future research.
Epidemiological Study of Pathogenic Leptospira in Raccoons (Procyon lotor) in a Suburb of Tokyo, Japan
Authors: Kiuno Kazuki , Kato Takuya , Otsubo Hiroko , Kibe Ryoko , Kataoka Yasushi , Hayama Shin-ichi
Published: December 2022

Leptospirosis is a zoonosis that affects humans and animals worldwide. Raccoons (Procyon lotor), adopted in urban environments, may act as potential reservoirs of Leptospira. We investigated the prevalence of pathogenic Leptospira in the kidney and urine samples of raccoons living in Tokyo, as well as anti-leptospiral antibodies in their serum, and aimed to examine the factors that expose raccoons to Leptospira. Polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) were used to detect leptospiral DNA and anti-leptospiral antibodies, respectively. Thirty-six of 156 raccoons (23.1%) were positive by PCR, and 16 of 165 raccoons (9.7%) were positive by ELISA. The prevalence and seroprevalence rates differed depending on the raccoon dispersal period. We used univariable logistic regression to estimate the environmental factors associated with pathogenic Leptospira and anti-leptospiral antibodies in raccoons. Significant differences were observed in the PCR results for the seasons (spring–summer) (p = 0.01), average monthly temperature (p < 0.01), and average monthly rainfall (p < 0.01). No significant difference was seen in the ELISA results, but raccoons in larger urban areas tended to have higher seroprevalence rates (p = 0.06). We identified a pattern of leptospiral spread in raccoon dispersal and environmental factors that expose raccoons to Leptospira.
Consistently Inconsistent Perceptual Illusions in Nonhuman Primates: The Importance of Individual Differences
Authors: Beran Michael J. , Parrish Audrey E.
Published: December 2022

Perceptual illusions, and especially visual illusions, are of great interest not only to scientists, but to all people who experience them. From a scientific perspective, illusory visual experiences are informative about the nature of visual processes and the translation of sensory experiences to perceptual information that can then be used to guide behavior. It has been widely reported that some nonhuman species share these illusory experiences with humans. However, it is consistently the case that not all members of a species experience illusions in the same way. In fact, individual differences in susceptibility may be more typical than universal experiences of any given illusion. Focusing on research with the same nonhuman primates who were given a variety of perceptual illusion tasks, this “consistent inconsistency” is clearly evident. Additionally, this can even be true in assessments of human illusory experiences. Individual differences in susceptibility offer an important avenue for better understanding idiosyncratic aspects of visual perception, and the goal of isolating any possible universal principles of visual perception (in primates and beyond) should address these individual differences.
Evaluation of Soil Moisture-Based Satellite Precipitation Products over Semi-Arid Climatic Region
Authors: Asif Muhammad , Nadeem Muhammad Umer , Anjum Muhammad Naveed , Ahmad Bashir , Manuchekhr Gulakhmadov , Umer Muhammad , Hamza Muhammad , Javaid Muhammad Mashood , Liu Tie
Published: December 2022

The ground validation of satellite-based precipitation products (SPPs) is very important for their hydroclimatic application. This study evaluated the performance assessment of four soil moisture-based SPPs (SM2Rain, SM2Rain- ASCAT, SM2Rain-CCI, and GPM-SM2Rain). All data of SPPs were compared with 64 weather stations in Pakistan from January 2005 to December 2020. All SPPs estimations were evaluated on daily, monthly, seasonal, and yearly scales, over the whole spatial domain, and at point-to-pixel scale. Widely used evaluation indices (root mean square error (RMSE), correlation coefficient (CC), bias, and relative bias (rBias)) along with categorical indices (false alarm ratio (FAR), probability of detection (POD), success ratio (SR), and critical success index (CSI) were evaluated for performance analysis. The results of our study signposted that: (1) On a monthly scale, all SPPs estimations were in better agreement with gauge estimations as compared to daily scales. Moreover, SM2Rain and GPM-SM2Rain products accurately traced the spatio-temporal variability with CC >0.7 and rBIAS within the acceptable range (±10) of the whole country. (2) On a seasonal scale (spring, summer, winter, and autumn), GPM-SM2Rain performed more satisfactorily as compared to all other SPPs. (3) All SPPs performed better at capturing light precipitation events, as indicated by the Probability Density Function (PDF); however, in the summer season, all SPPs displayed considerable over/underestimates with respect to PDF (%). Moreover, GPM-SM2RAIN beat all other SPPs in terms of probability of detection. Consequently, we suggest the daily and monthly use of GPM-SM2Rain and SM2Rain for hydro climate applications in a semi-arid climate zone (Pakistan).
A New Hematocrit Measurement Method Using a Chemiluminescence Biosensor and Its Application in a Chemiluminescence Immunoassay Platform for Myocardial Markers Detection with Whole Blood Samples
Authors: Zhao Huan , Han Hao , Lin Qifeng , Huang Li , Su Xiangyi , Fang Yile , Zhang Yuanying , Su Enben , Chen Zhu , Li Song , Deng Yan , He Nongyue
Published: December 2022

The accuracy and precision of analyte concentrations measured in whole blood by chemiluminescence immunoassay (CLIA) have been significantly affected by erythrocytes, which leads to poor application of whole blood CLIA in clinical practice. In this work, a chemiluminescence biosensing optical platform for blood hematocrit (HCT) analysis using MAGICL 6000 (Getein Biotechnology, Nanjing, China) was designed, implemented, and fully characterized. The developed method was successfully applied to determine various HCT levels of human blood from 0% to 65%, with a correlation coefficient of 0.9885 compared with the conventional method (Sysmex XE 5000, Kobe, Japan). A mathematical model was developed to quantitatively evaluate the impact of HCT on the results of two sample types (whole blood vs. plasma). Combining the established HCT method and mathematical model with CLIA on MAGICL 6000, the precision was significantly improved by almost 20%. Comparison studies using whole blood samples and corresponding plasma samples showed that the square of the correlation coefficients of troponin I (cTnI), myoglobin (MYO), creatine kinase MB (CK-MB), and N-terminal pro-hormone brain natriuretic peptide (NT-proBNP) were increased to 0.9992, 0.9997, 0.9996, and 0.9994, respectively, showing a great potential for clinical application.
Multifold Enhanced Raman Detection of Organic Molecules as Environmental Water Pollutants
Authors: Mu Yunyun , Liu Miao , Li Jiajun , Zhang Xinping
Published: December 2022

Organic molecules, including the benzene series, have been identified as pollutants in environmental water. Due to their very low solubility, they have very small concentrations in water, and they are difficult to be detected by conventional techniques. In particular, there is a lack of real-time, accurate, and rapid detection methods for such molecules in water. However, they are detrimental to human health in many aspects. Toluene has been an important indicator of such environmental pollution detections. In this work, we propose a 3D SERS scheme consisting of a hollow fiber that is coated on the inner wall with densely arranged silver nanoparticles, which supplies multifold Raman enhancement by the plasmonic microcavity. Strong confinement of excitation laser energy and strongly enhanced Raman signals with the bidirectional collection are utilized to achieve high-sensitivity detection of toluene molecules in water. Raman signal with a reasonable signal-to-noise ratio has been measured for a concentration of 0.53 mg/L, indicating a detection limit even lower than this value for such a Raman spectroscopic technique. The corresponding enhancement factor is higher than 6 × 103 with respect to the available systems. Thus, this device not only enables direct trace detection and real-time monitoring of the water-polluting status by organic molecules but also supplies a practical approach for biological sensing.
Futurization of Aging: Subjective Beliefs and Effects
Authors: Strizhitskaya Olga , Petrash Marina , Golubitskaya Daria , Kuzmina Maria , Krupina Kristina , Shchukin Anton , Engelgardt Elena
Published: December 2022

Aging in the face of an increasing population and growing life expectancy is considered one of the major demographic challenges in modern society. Previous research has revealed that quality of life in aging could significantly differ depending on the resources one possesses. However, little attention has been given to the mechanisms of formation of these resources and the role of intentionality. In the present study, we identified 22 strategies that favor a better life quality in aging and analyzed them from the perspective of subjective beliefs and reported performance. Our sample was adults (n = 72) aged 57–65, living in St. Petersburg, Russia. The results showed that although participants were aware of the strategies that favor aging, their reported performance ranged on a scale from average to infrequent use of these strategies. We found that subjective beliefs about the role of psychological resources for better aging predicted higher scores on subjective beliefs about the role of lifestyle resources and the reported performance of psychological resources. Our results suggest that there is a gap between subjective beliefs about the controllability of aging processes and the transformation of these beliefs into real performance.