bluuebunny/crossref_metadata_2025 huggingface dataset:
Epidemiological and Clinical Features of Streptococcus dysgalactiae ssp. equisimilis stG62647 and Other emm Types in Germany
Authors: Itzek Andreas , Weißbach Victoria , Meintrup David , Rieß Beate , van der Linden Mark , Borgmann Stefan
Published: April 2023

(1) Background: Streptococcus dysgalactiae subspecies equisimilis (SDSE) is an important β-hemolytic pathogen historically described as mainly affecting animals. Studies epidemiologically assessing the pathogenicity in the human population in Germany are rare. (2) Methods: the present study combines national surveillance data from 2010 to 2022 with a single-center clinical study conducted from 2016 to 2022, focusing on emm type, Lancefield antigen, antimicrobial resistance, patient characteristics, disease severity, and clinical infection markers. (3) Results: The nationwide reported invasive SDSE infections suggest an increasing infection burden for the German population. One particular emm type, stG62647, increased over the study period, being the dominant type in both study cohorts, suggesting a mutation-driven outbreak of a virulent clone. The patient data show that men were more affected than women, although in the single-center cohort, this trend was reversed for patients with stG62647 SDSE. Men affected by stG62647 developed predominantly fascial infections, whereas women suffering from superficial and fascial non-stG62647 SDSE infections were significantly younger than other patients. Increasing age was a general risk factor for invasive SDSE infections. (4) Conclusions: further studies are needed to further elucidate the raised questions regarding outbreak origin, underlying molecular mechanisms as well as sex-dependent pathogen adaptation.
Transmission Electron Microscopy Observation of Morphological Changes to Cryptophlebia Leucotreta Granulovirus following Ultraviolet Irradiation
Authors: Mwanza Patrick , Dealtry Gill , Lee Michael , Moore Sean
Published: April 2023

Cryptophlebia leucotreta granulovirus (CrleGV), a double-stranded DNA virus (genus Betabaculovirus, family Baculoviridae), is highly infective to the citrus insect pest Thaumatotibia leucotreta. The South African isolate CrleGV-SA is formulated into a commercial biopesticide and registered for use in several countries. In South Africa, it is used as a biopesticide in a multi-faceted integrated pest management approach for citrus crops involving chemical and biological control methods. The virus nucleocapsid is surrounded and protected by an occlusion body (OB) composed of granulin protein in a crystalline matrix. Like all other baculoviruses, CrleGV is susceptible to ultraviolet (UV) radiation from sunlight. This reduces its efficacy as a biopesticide in the field and necessitates frequent respraying. UV damage to baculovirus biopesticides is detected by means of functional bioassays. However, bioassays do not give an indication of whether any structural damage has occurred that may contribute to functional loss. In this study, transmission electron microscopy (TEM) was used to observe damage to the OB and nucleocapsid (NC) of CrleGV-SA, following controlled UV irradiation in the laboratory to mimic field conditions. The resultant images were compared with images of non-irradiated CrleGV-SA virus. TEM images of irradiated CrleGV-SA samples revealed changes to the OB crystalline faceting, a reduction in the size of the OBs, and damage to the NC following UV exposure for 72 h.
Heparin, Low Molecular Weight Heparin, and Non-Anticoagulant Derivatives for the Treatment of Inflammatory Lung Disease
Authors: Shute Janis Kay
Published: April 2023

Unfractionated heparin has multiple pharmacological activities beyond anticoagulation. These anti-inflammatory, anti-microbial, and mucoactive activities are shared in part by low molecular weight and non-anticoagulant heparin derivatives. Anti-inflammatory activities include inhibition of chemokine activity and cytokine synthesis, inhibitory effects on the mechanisms of adhesion and diapedesis involved in neutrophil recruitment, inhibition of heparanase activity, inhibition of the proteases of the coagulation and complement cascades, inhibition of neutrophil elastase activity, neutralisation of toxic basic histones, and inhibition of HMGB1 activity. This review considers the potential for heparin and its derivatives to treat inflammatory lung disease, including COVID-19, ALI, ARDS, cystic fibrosis, asthma, and COPD via the inhaled route.
Autochthonous Peruvian Natural Plants as Potential SARS-CoV-2 Mpro Main Protease Inhibitors
Authors: Peralta-Moreno Maria Nuria , Anton-Muñoz Vanessa , Ortega-Alarcon David , Jimenez-Alesanco Ana , Vega Sonia , Abian Olga , Velazquez-Campoy Adrian , Thomson Timothy M. , Granadino-Roldán José Manuel , Machicado Claudia , Rubio-Martinez Jaime
Published: April 2023

Over 750 million cases of COVID-19, caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), have been reported since the onset of the global outbreak. The need for effective treatments has spurred intensive research for therapeutic agents based on pharmaceutical repositioning or natural products. In light of prior studies asserting the bioactivity of natural compounds of the autochthonous Peruvian flora, the present study focuses on the identification SARS-CoV-2 Mpro main protease dimer inhibitors. To this end, a target-based virtual screening was performed over a representative set of Peruvian flora-derived natural compounds. The best poses obtained from the ensemble molecular docking process were selected. These structures were subjected to extensive molecular dynamics steps for the computation of binding free energies along the trajectory and evaluation of the stability of the complexes. The compounds exhibiting the best free energy behaviors were selected for in vitro testing, confirming the inhibitory activity of Hyperoside against Mpro, with a Ki value lower than 20 µM, presumably through allosteric modulation.
Peripheral Neuropathy in Systemic Autoimmune Rheumatic Diseases—Diagnosis and Treatment
Authors: De Souza Jean Marcos , Trevisan Thiago Junqueira , Sepresse Samara Rosa , Londe Ana Carolina , França Júnior Marcondes Cavalcante , Appenzeller Simone
Published: April 2023

Peripheral neuropathy (PN) is frequently observed in systemic rheumatic diseases and is a challenge in clinical practice. We aimed to review the evidence on the subject and proposed a comprehensive approach to these patients, facilitating diagnosis and management. We searched the MEDLINE database for the terms (and its respective Medical Subject Headings (MeSH) terms): “peripheral neuropathy” AND “rheumatic diseases” OR “systemic lupus erythematosus”, “rheumatoid arthritis”, “Sjogren syndrome”, and “vasculitis” from 2000 to 2023. This literature review focuses on the diagnostic workup of PNs related to systemic lupus erythematosus, Sjögren’s syndrome, rheumatoid arthritis, and systemic vasculitis. For every type of PN, we provide a pragmatic flowchart for diagnosis and also describe evidence-based strategies of treatment.
Antibody–Drug Conjugates for Multiple Myeloma: Just the Beginning, or the Beginning of the End?
Authors: Ray Upasana , Orlowski Robert Z.
Published: April 2023

Multiple myeloma is a malignancy of immunoglobulin-secreting plasma cells that is now often treated in the newly diagnosed and relapsed and/or refractory settings with monoclonal antibodies targeting lineage-specific markers used either alone or in rationally designed combination regimens. Among these are the anti-CD38 antibodies daratumumab and isatuximab, and the anti-Signaling lymphocytic activation molecule family member 7 antibody elotuzumab, all of which are used in their unconjugated formats. Single-chain variable fragments from antibodies also form a key element of the chimeric antigen receptors (CARs) in the B-cell maturation antigen (BCMA)-targeted CAR T-cell products idecabtagene vicleucel and ciltacabtagene autoleucel, which are approved in the advanced setting. Most recently, the bispecific anti-BCMA and T-cell-engaging antibody teclistamab has become available, again for patients with relapsed/refractory disease. Another format into which antibodies can be converted to exert anti-tumor efficacy is as antibody–drug conjugates (ADCs), and belantamab mafodotin, which also targets BCMA, represented the first such agent that gained a foothold in myeloma. Negative results from a recent Phase III study have prompted the initiation of a process for withdrawal of its marketing authorization. However, belantamab remains a drug with some promise, and many other ADCs targeting either BCMA or other plasma cell surface markers are in development and showing potential. This contribution will provide an overview of some of the current data supporting the possibility that ADCs will remain a part of our chemotherapeutic armamentarium against myeloma moving forward, and also highlight areas for future development.
N-Acetyl Cysteine Restores the Diminished Activity of the Antioxidant Enzymatic System Caused by SARS-CoV-2 Infection: Preliminary Findings
Authors: Soto María Elena , Manzano-Pech Linaloe , Palacios-Chavarría Adrían , Valdez-Vázquez Rafael Ricardo , Guarner-Lans Verónica , Pérez-Torres Israel
Published: April 2023

SARS-CoV-2 infects type II pneumocytes and disrupts redox homeostasis by overproducing reactive oxygen species (ROS). N-acetyl cysteine (NAC) is a precursor of the synthesis of glutathione (GSH) and it restores the loss of redox homeostasis associated to viral infections. The aim of the study is to evaluate the effect of the treatment with NAC on the enzymatic antioxidant system in serum from patients infected by SARS-CoV-2. We evaluated the enzymatic activities of thioredoxin reductase (TrxR), glutathione peroxidase (GPx), -S-transferase (GST), and reductase (GR) by spectrophotometry and the concentrations of the glutathione (GSH), total antioxidant capacity (TAC), thiols, nitrites (NO2–), and lipid peroxidation (LPO) in serum. The activity of the extracellular super oxide dismutase (ecSOD) was determined by native polyacrylamide gels, and 3-nitrotyrosine (3-NT) was measured by ELISA. A decrease in the activities of the ecSOD, TrxR, GPx, GST GR, (p = 0 ≤ 0.1), and the GSH, TAC, thiols, and NO2– (p ≤ 0.001) concentrations and an increase in LPO and 3-NT (p = 0.001) concentrations were found in COVID-19 patients vs. healthy subjects. The treatment with NAC as an adjuvant therapy may contribute to a reduction in the OS associated to the infection by SARS-CoV-2 through the generation of GSH. GSH promotes the metabolic pathways that depend on it, thus contributing to an increase in TAC and to restore redox homeostasis.
In Vitro and In Vivo Evaluation of a Bio-Inspired Adhesive for Bone Fixation
Authors: Schlund Matthias , Dartus Julien , Defrançois Sarah , Ferri Joël , Delattre Jérôme , Blanchemain Nicolas , Woisel Patrice , Lyskawa Joël , Chai Feng
Published: April 2023

Compared to metallic hardware, an effective bone adhesive can revolutionize the treatment of clinically challenging situations such as comminuted, articular, and pediatric fractures. The present study aims to develop such a bio-inspired bone adhesive, based upon a modified mineral-organic adhesive with tetracalcium phosphate (TTCP) and phosphoserine (OPS) by incorporating nanoparticles of polydopamine (nPDA). The optimal formulation, which was screened using in vitro instrumental tensile adhesion tests, was found to be 50%molTTCP/50%molOPS-2%wtnPDA with a liquid-to-powder ratio of 0.21 mL/g. This adhesive has a substantially stronger adhesive strength (1.0–1.6 MPa) to bovine cortical bone than the adhesive without nPDA (0.5–0.6 MPa). To simulate a clinical scenario of autograft fixation under low mechanical load, we presented the first in vivo model: a rat fibula glued to the tibia, on which the TTCP/OPS-nPDA adhesive (n = 7) was shown to be effective in stabilizing the graft without displacement (a clinical success rate of 86% and 71% at 5 and 12 weeks, respectively) compared to a sham control (0%). Significant coverage of newly formed bone was particularly observed on the surface of the adhesive, thanks to the osteoinductive property of nPDA. To conclude, the TTCP/OPS-nPDA adhesive fulfilled many clinical requirements for the bone fixation, and potentially could be functionalized via nPDA to offer more biological activities, e.g., anti-infection after antibiotic loading.
Amorphization of Ethenzamide and Ethenzamide Cocrystals—A Case Study of Single and Binary Systems Forming Low-Melting Eutectic Phases Loaded on/in Silica Gel
Authors: Trzeciak Katarzyna , Wielgus Ewelina , Kaźmierski Sławomir , Pawlak Tomasz , Potrzebowski Marek J.
Published: April 2023

The applicability of different solvent-free approaches leading to the amorphization of active pharmaceutical ingredients (APIs) was tested. Ethenzamide (ET), an analgesic and anti-inflammatory drug, and two ethenzamide cocrystals with glutaric acid (GLU) and ethyl malonic acid (EMA) as coformers were used as pharmaceutical models. Calcinated and thermally untreated silica gel was applied as an amorphous reagent. Three methods were used to prepare the samples: manual physical mixing, melting, and grinding in a ball mill. The ET:GLU and ET:EMA cocrystals forming low-melting eutectic phases were selected as the best candidates for testing amorphization by thermal treatment. The progress and degree of amorphousness were determined using instrumental techniques: solid-state NMR spectroscopy, powder X-ray diffraction, and differential scanning calorimetry. In each case, the API amorphization was complete and the process was irreversible. A comparative analysis of the dissolution profiles showed that the dissolution kinetics for each sample are significantly different. The nature and mechanism of this distinction are discussed.
Magnetic Gels in Skin Cancer Treatment: A Review of Potential Applications in Diagnostics, Drug Delivery and Hyperthermia
Authors: Bruschi Marcos Luciano , de Souza Nunes Glécilla Colombelli
Published: April 2023

Skin cancer (SC) is affecting an increasing number of people worldwide. Its lesions affect mainly the most exposed regions of the skin. SC is classified into to main categories: non-melanoma (basal cell carcinoma of the epidermis and squamous cell carcinoma) and melanoma (the abnormal proliferation of melanocytes, which is rarer, more hazardous, and more deadly). Prevention and early diagnosis are important actions, and surgery is often considered. After the removal of cancerous lesions, the local administration of medicine can guarantee anticancer therapeutic action, rapid healing and the recovery of tissue, ensuring the absence of recurrence. Magnetic gels (MGs) have attracted increased attention regarding their pharmaceutical and biomedical applications. They are magnetic nanoparticles (e.g., iron oxide nanoparticles) dispersed in a polymeric matrix, which constitute adaptive systems under a magnetic field. MGs can combine magnetic susceptibility, high elasticity, and softness, and are thus useful platforms for diagnostics, drug delivery, and also for hyperthermia. This manuscript reviews MGs as a technological strategy for the treatment of SC. An overview of SC and the treatment, types, and methods of preparing MGs are discussed. Moreover, the applications of MGs in SC and their future perspectives are considered. The combination of polymeric gels and magnetic nanoparticles continues to be investigated, and new products must hit the market. Clinical trials and new products are expected, due to the important advantages of MGs.
Development of Nanofibers with Embedded Liposomes Containing an Immunomodulatory Drug Using Green Electrospinning
Authors: Casula Luca , Zidar Anže , Kristl Julijana , Jeras Matjaž , Kralj Slavko , Fadda Anna Maria , Zupančič Špela
Published: April 2023

Conventional treatments for chronic wounds are often ineffective, thus new therapeutic approaches are needed, such as the delivery of immunomodulatory drugs that can reduce inflammation, restore immune cell function, and facilitate tissue regeneration. A potential drug for such an approach is simvastatin, which has major drawbacks including poor solubility and chemical instability. With the aim of developing a dressing for wound healing, simvastatin and an antioxidant were incorporated into alginate/poly(ethylene oxide) nanofibers by green electrospinning without the use of organic solvents, thanks to their prior encapsulation into liposomes. The composite liposome–nanofiber formulations exhibited fibrillar morphology (160–312 nm) and unprecedentedly high phospholipid and drug content (76%). Transmission electron microscopy revealed dried liposomes as bright ellipsoidal spots homogeneously distributed over the nanofibers. After nanofiber hydration, the liposomes reconstituted in two size populations (~140 and ~435 nm), as revealed by cutting-edge MADLS® analysis. Lastly, in vitro assays demonstrated that composite liposome–nanofiber formulations are superior to liposomal formulations due to a better safety profile in keratinocytes and peripheral blood mononuclear cells. Furthermore, both formulations exhibited similarly advantageous immunomodulatory effects, measured as decreased inflammation in vitro. A synergistic combination of the two nanodelivery systems shows promise for the development of efficient dressings for chronic wound treatment.
Risk Factors for Bloodstream Infections Due to ESBL-Producing Escherichia coli, Klebsiella spp., and Proteus mirabilis
Authors: Vance Mary Kathryn , Cretella David A. , Ward Lori M. , Vijayvargiya Prakhar , Garrigos Zerelda Esquer , Wingler Mary Joyce B.
Published: April 2023

(1) Background: Risk factors for extended-spectrum beta-lactamase (ESBL) infections could vary geographically. The purpose of this study was to identify local risk factors for ESBL production in patients with Gram-negative bacteremia. (2) Methods: This retrospective observational study included adult patients admitted from January 2019 to July 2021 and had positive blood cultures for E. coli, K. pneumoniae, K. oxytoca, and P. mirabilis. Patients with ESBL infection were matched to a non-ESBL-producing infection with the same organism. (3) Results: A total of 150 patients were included: 50 in the ESBL group and 100 in the non-ESBL group. Patients in the ESBL group had a longer length of stay (11 vs. 7 days, p < 0.001), but not increased mortality (14% vs. 15%, p = 0.87) Multivariate analysis identified the receipt of >1 antibiotic in the last 90 days as a risk factor for ESBL infection (OR = 3.448, 95% CI = 1.494–7.957; p = 0.004); (4) Conclusions: Recent antimicrobial use was identified as an independent risk factors for ESBL-producing Enterobacterales infections. Knowledge of this risk may improve empirical therapy and reduce inappropriate use.
Rate-Splitting-Based Generalized Multiple Access for Band-Limited Multi-User VLC
Authors: Tang Yuru , Chen Chen , Liu Min , Du Pengfei , Fu H. Y.
Published: April 2023

In this paper, we propose a rate-splitting-based generalized multiple access (GMA) scheme for band-limited multi-user visible light communication (VLC) systems. By splitting and transmitting the input data of each user in a joint orthogonal multiple access (OMA) and non-orthogonal multiple access (NOMA) manner, the proposed rate-splitting-based GMA scheme can obtain better bandwidth utilization than OMA and suffer less severe interference than NOMA. In order to achieve the maximum sum rate over typical low-pass VLC channels, the optimal rate-splitting-based GMA scheme was first obtained through theoretical analysis and computer simulations. Subsequently, the superiority of the optimal rate-splitting-based GMA scheme over both OMA and NOMA under various channel conditions, user separations, and error propagation levels was further verified by the theoretical, simulation, and experimental results. In particular, the experimental results showed that, when the error propagation ratio was increased from 0 to 0.2, the sum rate reduction ratio was significantly reduced from 31.4% to 7.5% by replacing NOMA with the obtained optimal rate-splitting-based GMA.
Prism Couplers with Convex Output Surfaces for Nonlinear Cherenkov Terahertz Generation
Authors: Kitaeva Galiya Kh. , Markov Dmitrii A. , Safronenkov Daniil A. , Starkova Natalia V.
Published: April 2023

Sideways THz generation in Mg:LiNbO3 crystal is studied considering Si-prism-lens couplers with different output surface curvatures. A theoretical approach is developed for modeling the angular distributions of THz radiation power inside the crystal, inside the Si coupler and outside in free space. Our calculations show how the imposition of a plano-convex lens on the standard flat prism can substantially improve the THz generation efficiency. The ratio between the lens curvature radius and the distance from the curvature center to the point of generation on the lens axis is found to be one of the most important parameters. The developed general approach can be used for the further design of the optimal THz extraction elements of a different configuration.
Demonstration of 12.5 Mslot/s 32-PPM Underwater Wireless Optical Communication System with 0.34 Photons/Bit Receiver Sensitivity
Authors: Han Xiaotian , Li Peng , Li Guangying , Chang Chang , Jia Shuaiwei , Xie Zhuang , Liao Peixuan , Nie Wenchao , Xie Xiaoping
Published: April 2023

High-capacity, long-distance underwater wireless optical communication (UWOC) technology is an important component in building fast, flexible underwater sensing networks. Underwater communication with light as a carrier has a large communication capacity, but channel loss induced by light attenuation and scattering largely limits the underwater wireless optical communication distance. To improve the communication distance, a low-power 450 nm blue continuous wave (CW) laser diode (LD)-based UWOC system was proposed and experimentally demonstrated. A communication link was designed and constructed with a BER of 3.6 × 10−3 in a total link loss of 80.72 dB in c = 0.51 m−1 water with a scintillation index (S.I.) equal to 0.02 by combining with 32-pulse-position modulation (32-PPM) at a bandwidth of 12.5 MHz and single photon counting reception techniques. The allowable underwater communication distance in Jerlov II (c = 0.528 m−1) water was estimated to be 35.64 m. The attenuation lengths were 18.82, which were equal at link distances of 855.36 m in Jerlov I (c = 0.022 m−1) water. A receiving sensitivity of 0.34 photons/bit was achieved. To our knowledge, this is the lowest receiving sensitivity ever reported under 0.1 dB of signal-to-noise ratio (SNR) in the field of UWOC.
Effects of Ca Sprays on Fruit Ca Content and Yield of Tomato Variety Susceptible to Blossom-End Rot
Authors: Karlsons Andis , Osvalde Anita , Cekstere Gunta , Āboliņa Laura
Published: April 2023

Several factors are involved in the incidence of blossom-end rot (BER) in tomato fruit, but the main one is insufficient Ca uptake and transport through the plant, resulting in Ca deficiency in the fruit. Sprays of Ca-containing products are considered to be a possible measure to overcome the local Ca deficiency in tomato fruit. Therefore, the main objective was to evaluate the effectiveness of additional Ca supply to tomato fruits for increasing Ca content and reducing fruit damage. Sprays of five different commercial preparations containing (Brexil Duo, Calmax Zero N, Ca(NO3)2, CaCl2) or promoting (Greenstim) Ca uptake were tested using BER-sensitive large-fruit variety ‘Beorange’. The experiment was conducted in the commercial greenhouse ‘Getlini EKO’, Latvia, during the autumn–spring season of 2020/2021 under controlled conditions, eliminating the adverse impact of external factors. The results revealed that none of the preparations were effective in increasing Ca content, preventing BER, and did not promote the tomato yield. As good agricultural practices were followed in the greenhouse to manage BER, we concluded that a non-marketable yield of around 15% should be expected for ‘Beorange’ when grown under artificial light, possibly due to the impact of abiotic stresses and genetically determined susceptibility.
Identification of Novel QTLs Associated with Frost Tolerance in Winter Wheat (Triticum aestivum L.)
Authors: Bolouri Parisa , Haliloğlu Kamil , Mohammadi Seyyed Abolghasem , Türkoğlu Aras , İlhan Emre , Niedbała Gniewko , Szulc Piotr , Niazian Mohsen
Published: April 2023

Low temperature (cold) and freezing stress is a major problem during winter wheat growth. Low temperature tolerance (LT) is an important agronomic trait in winter wheat and determines the plants’ ability to cope with below-freezing temperatures; thus, the development of cold-tolerant cultivars has become a major goal of breeding in various regions of the world. In this study, we sought to identify quantitative trait loci (QTL) using molecular markers related to freezing tolerance in winter. Thirty-four polymorphic markers among 425 SSR markers were obtained for the population, including 180 inbred lines of F12 generation wheat, derived from crosses (Norstar × Zagros) after testing with parents. LT50 is used as an effective selection criterion for identifying frost-tolerance genotypes. The progeny of individual F12 plants were used to evaluate LT50. Several QTLs related to wheat yield, including heading time period, 1000-seed weight, and number of surviving plants after overwintering, were identified. Single-marker analysis illustrated that four SSR markers with a total of 25% phenotypic variance determination were linked to LT50. Related QTLs were located on chromosomes 4A, 2B, and 3B. Common QTLs identified in two cropping seasons based on agronomical traits were two QTLs for heading time period, one QTL for 1000-seed weight, and six QTLs for number of surviving plants after overwintering. The four markers identified linked to LT50 significantly affected both LT50 and yield-related traits simultaneously. This is the first report to identify a major-effect QTL related to frost tolerance on chromosome 4A by the marker XGWM160. It is possible that some QTLs are closely related to pleiotropic effects that control two or more traits simultaneously, and this feature can be used as a factor to select frost-resistant lines in plant breeding programs.
Transcriptional Expression of Nitrogen Metabolism Genes and Primary Metabolic Variations in Rice Affected by Different Water Status
Authors: Kim Gahyun , Sung Jwakyung
Published: April 2023

The era of climate change strongly requires higher efficiency of energies, such as light, water, nutrients, etc., during crop production. Rice is the world’s greatest water-consuming plant, and, thus, water-saving practices such as alternative wetting and drying (AWD) are widely recommended worldwide. However the AWD still has concerns such as lower tillering, shallow rooting, and an unexpected water deficit. The AWD is a possibility to not only save water consumption but also utilize various nitrogen forms from the soil. The current study tried to investigate the transcriptional expression of genes in relation to the acquisition-transportation-assimilation process of nitrogen using qRT-PCR at the tillering and heading stages and to profile tissue-specific primary metabolites. We employed two water supply systems, continuous flooding (CF) and alternative wetting and drying (AWD), during rice growth (seeding to heading). The AWD system is effective at acquiring soil nitrate; however, nitrogen assimilation was predominant in the root during the shift from the vegetative to the reproductive stage. In addition, as a result of the greater amino acids in the shoot, the AWD was likely to rearrange amino acid pools to produce proteins in accordance with phase transition. Accordingly, it is suggested that the AWD 1) actively acquired nitrate from soil and 2) resulted in an abundance of amino acid pools, which are considered a rearrangement under limited N availability. Based on the current study, further steps are necessary to evaluate form-dependent N metabolism and root development under the AWD condition and a possible practice in the rice production system.
Review on Propolis Applications in Food Preservation and Active Packaging
Authors: Segueni Narimane , Boutaghane Naima , Asma Syeda Tasmia , Tas Nuri , Acaroz Ulas , Arslan-Acaroz Damla , Shah Syed Rizwan Ali , Abdellatieff Hoda A. , Akkal Salah , Peñalver Rocío , Nieto Gema
Published: April 2023

Propolis is a natural hive product collected by honeybees from different plants and trees. The collected resins are then mixed with bee wax and secretions. Propolis has a long history of use in traditional and alternative medicine. Propolis possesses recognized antimicrobial and antioxidant properties. Both properties are characteristics of food preservatives. Moreover, most propolis components, in particular flavonoids and phenolic acids, are natural constituents of food. Several studies suggest that propolis could find use as a natural food preservative. This review is focused on the potential application of propolis in the antimicrobial and antioxidant preservation of food and its possible application as new, safe, natural, and multifunctional material in food packaging. In addition, the possible influence of propolis and its used extracts on the sensory properties of food is also discussed.
Effects of Ammonia on Juvenile Sunray Surf Clam (Mactra chinensis Philippi) in Laboratory Tests
Authors: Dai Yuanyuan , Dong Yubo , Yang Feng , Chen Zhongzhi , Jia Jia
Published: April 2023

The current study aimed to determine the acute and sub-chronic toxicity of ammonia to juvenile surf clams (Mactra chinensis Philippi). Acute toxicity tests were conducted with seven concentrations of ammonium chloride using a 96 h static-renewal approach. Sub-chronic ammonia exposure tests (20 d exposures) were conducted with 6 concentrations at 20 °C. The 96 h median lethal concentration (96 h LC50) was 11.1 (10.0; 12.0) mg/L total ammonia nitrogen (TAN) and 0.56 (0.50; 0.60) mg/L unionized ammonia (NH3). The relative growth rate was significantly reduced at concentrations higher than 1.6 mg/L TAN (0.075 mg/L NH3) in the 20 d tests. The estimated maximum acceptable toxicant concentration (MATC) based on the reduced growth of juvenile M. chinensis was between 0.8 and1.6 mg/L TAN (0.038–0.075 mg/L NH3). Histopathological changes were evaluated in the surviving clams after 20 days of exposure. Exposure to 14.1 mg/L TAN (0.661 mg/L NH3) resulted in changes in the mantle, foot and digestive diverticulum. We also examined the antioxidant enzyme activities of superoxide dismutase (SOD) and catalase (CAT) in 10 d and 20 d at 6 different levels (plus a control) of ammonia from 0.8 mg/L to 14.1 mg/L TAN. Ammonia exposure at 0.8 mg/L TAN (0.038 mg/L NH3) significantly affected SOD and CAT activities. The level of enzymic activity decreased with the increasing concentration of TAN. The results improved our understanding of oxidative damage under ammonia exposure and provided data for the aquaculture of sunray surf clams.
Study on Dynamic Modulus and Damping Characteristics of Modified Expanded Polystyrene Lightweight Soil under Cyclic Load
Authors: Tao Huaqiang , Zheng Wenqian , Zhou Xuhui , Zhou Lin , Li Cuihong , Yu Yanfei , Jiang Ping
Published: April 2023

In recent years, expanded polystyrene (EPS) lightweight soil has been widely used as subgrade in soft soil areas because of its light weight and environmental protection. This study aimed to investigate the dynamic characteristics of sodium silicate modified lime and fly ash treated EPS lightweight soil (SLS) under cyclic loading. The effects of EPS particles on the dynamic elastic modulus (Ed) and damping ratio (λ) of SLS were determined through dynamic triaxial tests at various confining pressures (σ3), amplitudes, and cycle times. Mathematical models of the Ed of the SLS, cycle times, and σ3 were established. The results revealed that the EPS particle content played a decisive role in the Ed and λ of the SLS. The Ed of the SLS decreased with an increase in the EPS particle content (EC). The Ed decreased by 60% in the 1–1.5% range of the EC. The existing forms of lime fly ash soil and EPS particles in the SLS changed from parallel to series. With an increase in σ3 and amplitude, the Ed of the SLS gradually decreased, the λ generally decreased, and the λ variation range was within 0.5%. With an increase in the number of cycles, the Ed of the SLS decreased. The Ed value and the number of cycles satisfied the power function relationship. Additionally, it can be found from the test results that 0.5% to 1% was the best EPS content for SLS in this work. In addition, the dynamic elastic modulus prediction model established in this study can better describe the varying trend of the dynamic elastic modulus of SLS under different σ3 values and load cycles, thereby providing a theoretical reference for the application of SLS in practical road engineering.
Chondrogenic Differentiation of Adipose-Derived Stromal Cells Induced by Decellularized Cartilage Matrix/Silk Fibroin Secondary Crosslinking Hydrogel Scaffolds with a Three-Dimensional Microstructure
Authors: Zhou Jing , Wu Nier , Zeng Jinshi , Liang Ziyu , Qi Zuoliang , Jiang Haiyue , Chen Haifeng , Liu Xia
Published: April 2023

Finding an ideal scaffold is always an important issue in the field of cartilage tissue engineering. Both decellularized extracellular matrix and silk fibroin have been used as natural biomaterials for tissue regeneration. In this study, a secondary crosslinking method of γ irradiation and ethanol induction was used to prepare decellularized cartilage extracellular matrix and silk fibroin (dECM-SF) hydrogels with biological activity. Furthermore, the dECM-SF hydrogels were cast in custom-designed molds to produce a three-dimensional multi-channeled structure to improve internal connectivity. The adipose-derived stromal cells (ADSC) were seeded on the scaffolds, cultured in vitro for 2 weeks, and implanted in vivo for another 4 and 12 weeks. The double crosslinked dECM-SF hydrogels exhibited an excellent pore structure after lyophilization. The multi-channeled hydrogel scaffold presents higher water absorption ability, surface wettability, and no cytotoxicity. The addition of dECM and a channeled structure could promote chondrogenic differentiation of ADSC and engineered cartilage formation, confirmed by H&E, safranin O staining, type II collagen immunostaining, and qPCR assay. In conclusion, the hydrogel scaffold fabricated by the secondary crosslinking method has good plasticity and can be used as a scaffold for cartilage tissue engineering. The multi-channeled dECM-SF hydrogel scaffolds possess a chondrogenic induction activity that promotes engineered cartilage regeneration of ADSC in vivo.
Influence of the Addition of Vital Wheat Gluten on Thermal and Rheological Properties of Triticale Flour
Authors: Pycia Karolina , Kaszuba Joanna , Posadzka Zuzanna , Juszczak Lesław
Published: April 2023

The aim of this study was to evaluate the effect of the addition of vital wheat gluten to triticale flour on its thermal and rheological properties. In the tested systems (TG), triticale flour from Belcanto grain was replaced with vital wheat gluten in the amounts of 1%, 2%, 3%, 4% and 5%. Wheat flour (WF) and triticale flour (TF) were also tested. For the tested flours and mixtures with gluten, the falling number, gluten content, as well as the parameters of gelatinization and retrogradation characteristics using differential scanning calorimetry (DSC) and characteristics of pasting using a viscosity analyzer (RVA) were determined. In addition, viscosity curves were plotted, and viscoelastic properties of the obtained gels were also assessed. It was observed that there were no statistically significant differences between the TF and TG samples in terms of falling number. The average value of this parameter in TG samples was 317 s. It was found that the replacement of TF with vital gluten reduced the gelatinization enthalpy and increased the retrogradation enthalpy, as well as the degree of retrogradation. The highest viscosity was characterized by the WF paste (1784 mPa·s) and the lowest by the TG5% mixture (1536 mPa·s). Replacing TF with gluten resulted in a very visible decrease in the apparent viscosity of the systems. In addition, the gels based on the tested flours and TG systems had the character of weak gels (tan δ = G″/G′ > 0.1), while the values of the parameters G′ and G″ decreased as the share of gluten in the systems increased.
Proposal of Evaluation Method for Crack Propagation Behaviors of Second-Generation Acrylic Adhesives under Mode I Static Loading
Authors: Ogawa Yuki , Naito Kimiyoshi , Harada Keisuke , Oguma Hiroyuki
Published: April 2023

Second-generation acrylic (SGA) adhesives, possessing high strength and toughness, are applicable in automotive body structures. Few studies have considered the fracture toughness of the SGA adhesives. This study entailed a comparative analysis of the critical separation energy for all three SGA adhesives and an examination of the mechanical properties of the bond. Loading-unloading test was performed to evaluate crack propagation behaviors. In the loading–unloading test of the SGA adhesive with high ductility, plastic deformation was observed in the steel adherends; the arrest load dominated the propagation and non-propagation of crack for adhesive. The critical separation energy of this adhesive was assessed by the arrest load. In contrast, for the SGA adhesives with high tensile strength and modulus, the load suddenly decreased during loading, and the steel adherend was not plastically deformed. The critical separation energies of these adhesives were assessed using the inelastic load. The critical separation energies for all the adhesives were higher for thicker adhesive. Particularly, the critical separation energies of the highly ductile adhesives were more affected by the adhesive thickness than highly strength adhesives. The critical separation energy from the analysis using the cohesive zone model agreed with the experimental results.
Process Parameter Prediction for Fused Deposition Modeling Using Invertible Neural Networks
Authors: Pelzer Lukas , Posada-Moreno Andrés Felipe , Müller Kai , Greb Christoph , Hopmann Christian
Published: April 2023

Additive manufacturing has revolutionized prototyping and small-scale production in the past years. By creating parts layer by layer, a tool-less production technology is established, which allows for rapid adaption of the manufacturing process and customization of the product. However, the geometric freedom of the technologies comes with a large number of process parameters, especially in Fused Deposition Modeling (FDM), all of which influence the resulting part’s properties. Since those parameters show interdependencies and non-linearities, choosing a suitable set to create the desired part properties is not trivial. This study demonstrates the use of Invertible Neural Networks (INN) for generating process parameters objectively. By specifying the desired part in the categories of mechanical properties, optical properties and manufacturing time, the demonstrated INN generates process parameters capable of closely replicating the desired part. Validation trials prove the precision of the solution with measured properties achieving the desired properties to up to 99.96% and a mean accuracy of 85.34%.
Bio-Based Photoreversible Networks Containing Coumarin Groups for Future Medical Applications
Authors: Elchiev Iskenderbek , Demirci Gokhan , El Fray Miroslawa
Published: April 2023

Photocurable biomaterials that can be delivered as liquids and rapidly (within seconds) cured in situ using UV light are gaining increased interest in advanced medical applications. Nowadays, fabrication of biomaterials that contain organic photosensitive compounds have become popular due to their self-crosslinking and versatile abilities of changing shape or dissolving upon external stimuli. Special attention is paid to coumarin due to its excellent photo- and thermoreactivity upon UV light irradiation. Thus, by modifying the structure of coumarin to make it reactive with a bio-based fatty acid dimer derivative, we specifically designed a dynamic network that is sensitive to UV light and able to both crosslink and re-crosslink upon variable wave lengths. A simple condensation reaction was applied to obtain future biomaterial suitable for injection and photocrosslinking in situ upon UV light exposure and decrosslinking at the same external stimuli but at different wave lengths. Thus, we performed the modification of 7-hydroxycoumarin and condensation with fatty acid dimer derivatives towards a photoreversible bio-based network for future medical applications.
Significant Improvement of Thermal Conductivity of Polyamide 6/Boron Nitride Composites by Adding a Small Amount of Stearic Acid
Authors: Fang Hui , Li Guifeng , Wang Kai , Wu Fangjuan
Published: April 2023

This study investigates the effect of adding stearic acid (SA) on the thermal conductivity of polyamide 6 (PA6)/boron nitride (BN) composites. The composites were prepared by melt blending, and the mass ratio of PA6 to BN was fixed at 50:50. The results show that when the SA content is less than 5 phr, some SA is distributed at the interface between BN sheets and PA6, which improves the interface adhesion of the two phases. This improves the force transfer from the matrix to BN sheets, promoting the exfoliation and dispersion of BN sheets. However, when the SA content was greater than 5 phr, SA tends to aggregate and form separate domains rather than being dispersed at the interface between PA6 and BN. Additionally, the well-dispersed BN sheets act as a heterogeneous nucleation agent, significantly improving the crystallinity of the PA6 matrix. The combination of good interface adhesion, excellent orientation, and high crystallinity of the matrix leads to efficient phonon propagation, resulting in a significant improvement in the thermal conductivity of the composite. The highest thermal conductivity of the composite is achieved when the SA content is 5 phr, which is 3.59 W m−1 K−1. The utilization of a composite material consisting of 5phr SA as the thermal interface material displays the highest thermal conductivity, and the composite also demonstrates satisfactory mechanical properties. This study proposes a promising strategy for the preparation of composites with high thermal conductivity.
An Extension of the Poisson Distribution: Features and Application for Medical Data Modeling
Authors: El-Dawoody Mohamed , Eliwa Mohamed S. , El-Morshedy Mahmoud
Published: April 2023

This paper introduces and studies a new discrete distribution with one parameter that expands the Poisson model, discrete weighted Poisson Lerch transcendental (DWPLT) distribution. Its mathematical and statistical structure showed that some of the basic characteristics and features of the DWPLT model include probability mass function, the hazard rate function for single and double components, moments with auxiliary statistical measures (expectation, variance, index of dispersion, skewness, kurtosis, negative moments), conditional expectation, Lorenz function, and order statistics, which were derived as closed forms. DWPLT distribution can be used as a flexible statistical approach to analyze and discuss real asymmetric leptokurtic data. Moreover, it could be applied to a hyperdispersive data model. Two different estimation methods were derived, i.e., maximal likelihood and the moments technique for the DWPLT parameter, and some advanced numerical methods were utilized for the estimation process. A simulation was performed to examine and analyze the performance of the DWPLT estimator on the basis of the criteria of the bias and mean squared errors. The flexibility and fit ability of the proposed distribution is demonstrated via the clinical application of a real dataset. The DWPLT model was more flexible and worked well for modeling real age data when compared to other competitive age distributions in the statistical literature.
Green Innovation under the Constraint of Economic Growth Targets: Evidence from Prefecture Level Cities in China
Authors: Ma Tao , Wang Shuchen
Published: April 2023

The demand for sustainable economic growth highlights the trade off between environmental and economic targets. From the perspective of economic growth target (EGT) management and green innovation (GI) practice, in this study, we constructed dynamic panel, spatial Dubin, quantile, and threshold models to measure the impact of EGT on GI using the panel data of 284 prefecture cities in China from 2006 to 2018. The results show that EGT has a negative impact on GI, which is characterized by dynamic, superposition, spatial, and nonlinear effects; there is remarkable heterogeneity in different regions, development stages, and urban characteristics, and the empirical conclusion is still credible under many robustness tests. We also studied the heterogeneous impact of economic growth targets with different characteristics on green innovation. This study puts forward policy implications from two perspectives: optimizing top-level design and maximizing the trade off in multi-objective accountability.
Influence of Exhaust Pipe Structure on Urea-Related Deposits in Diesel Engine SCR System
Authors: Zhu Neng , Song Hang , Qian Feng , Xu Xiaowei
Published: April 2023

To solve the problem of deposits formation in the exhaust pipe of a diesel engine SCR (selective catalytic reduction) system, the CFD (computational fluid dynamics) model of the exhaust pipe was established to research the influence of structural parameters on the wall film from the perspective of optimizing the flow field. The solid structure was simplified in the modeling without considering the flow fields inside the catalytic converter. The simulation conditions were obtained through bench tests and vehicle real road tests. The spray and temperature simulation results were verified via high-speed photographic and bench tests under three typical operating conditions. The conclusions are as follows: compared to the case with a step surface, wall film mass was reduced by 48.27~55.4% when the exhaust pipe had no step surface; compared to the case where the nozzle orifices were located 10 mm off center axis, wall film mass reduced by 2.83~6.38% when the nozzle orifices located in the center axis of exhaust pipe; compared to the case of 110 mm, wall film mass reduced by 21.3~24.72% when the exhaust pipe diameter was 100 mm. To eliminate the potential problem of deposit formation inside the catalytic converter, the nozzle should be arranged close to the engine turbine, and there should be no abrupt cross-section downstream of the exhaust pipe.
Special Issue on “Renewable Energy Technologies and Systems: Technical, Environmental, Economic, Social, and Cultural Challenges”
Authors: Cunha Jorge , Ferreira Paula , Cai Wei
Published: April 2023

Due to the increasing pressures on the environment derived from the intensive use of energy, policy-makers are increasingly aware of the need to design new energy policies capable of addressing those negative impacts on the environment [...]
An Actor-Critic Algorithm for the Stochastic Cutting Stock Problem
Authors: Su Jie-Ying , Kang Jia-Lin , Jang Shi-Shang
Published: April 2023

The inventory level has a significant influence on the cost of process scheduling. The stochastic cutting stock problem (SCSP) is a complicated inventory-level scheduling problem due to the existence of random variables. In this study, we applied a model-free on-policy reinforcement learning (RL) approach based on a well-known RL method, called the Advantage Actor-Critic, to solve a SCSP example. To achieve the two goals of our RL model, namely, avoiding violating the constraints and minimizing cost, we proposed a two-stage discount factor algorithm to balance these goals during different training stages and adopted the game concept of an episode ending when an action violates any constraint. Experimental results demonstrate that our proposed method obtains solutions with low costs and is good at continuously generating actions that satisfy the constraints. Additionally, the two-stage discount factor algorithm trained the model faster while maintaining a good balance between the two aforementioned goals.