bluuebunny/crossref_metadata_2025 huggingface dataset:
The Moderating Effect of Family Business Ownership on the Relationship between Short-Selling Mechanism and Firm Value for Listed Companies in China
Authors: Mai Wenzhen , Hamid Nik Intan Norhan Binti Abdul
Published: May 2021

This study demonstrates an investigation of the external corporate governance effect of short selling mechanisms on firm value in the Chinese context. The effect of family businesses is also examined as a moderator of the relationship between short-selling and firm value. Using panel data analysis of Chinese listed companies, this paper tests a total sample of 22,468 firm-year observations from the Shanghai and Shenzhen Stock Exchange from 2009 to 2019 by applying the PSM-DID method in order to mitigate self-selection and endogenous problems caused by the uniqueness of Chinese short selling mechanisms. The findings suggest that both deregulation and the propensity of short selling can improve the firm value. Our findings also established that family ownership weakens firm value with the availability of short-selling, which indicates that family businesses have long orientations and conduct better corporate governance practices than non-family business, as short-selling shows a weaker external governance effect on firm value creation by family businesses in China. A robust test of alternative measurements is conducted and validated. This study provides significant insights for policymakers to consider in order to further relax short-selling constraints, which can act as effective external governance for better firm value creation, especially for non-family businesses in developing countries.
Comparing Hydric Erosion Soil Loss Models in Rainy Mountainous and Dry Flat Regions in Portugal
Authors: Duarte Lia , Cunha Mário , Teodoro Ana Cláudia
Published: May 2021

Soil erosion is a severe and complex issue in the agriculture area. The main objective of this study was to assess the soil loss in two regions, testing different methodologies and combining different factors of the Revised Universal Soil Loss Equation (RUSLE) based on Geographical Information Systems (GIS). To provide the methodologies to other users, a GIS open-source application was developed. The RUSLE equation was applied with the variation of some factors that compose it, namely the slope length and slope steepness (LS) factor and practices factor (P), but also with the use of different sources of information. Eight different erosion models (M1 to M8) were applied to the two regions with different ecological conditions: Montalegre (rainy-mountainous) and Alentejo (dry-flat), both in Portugal, to compare them and to evaluate the soil loss for 3 potential erosion levels: 0–25, 25–50 and >50 ton/ha·year. Regarding the methodologies, in both regions the behavior is similar, indicating that the M5 and M6 methodologies can be more conservative than the others (M1, M2, M3, M4 and M8), which present very consistent values in all classes of soil loss and for both regions. All methodologies were implemented in a GIS application, which is free and available under QGIS software.
Dynamic Landscape Connectivity Special Issue Editorial
Authors: Jennings Megan K. , Zeller Katherine A. , Lewison Rebecca L.
Published: May 2021

Until fairly recently, the majority of landscape connectivity analyses have considered connectivity as a static landscape feature, despite the widespread recognition that landscapes and the abiotic and biotic processes that influence them are dynamic [...]
Land Use Change, Urban Agglomeration, and Urban Sprawl: A Sustainable Development Perspective of Makassar City, Indonesia
Authors: Surya Batara , Salim Agus , Hernita Hernita , Suriani Seri , Menne Firman , Rasyidi Emil Salim
Published: May 2021

Urbanization towards the expansion of the city area causes urban sprawl and changes in space use. Furthermore, urban agglomeration towards urban spatial integration causes a decrease in environmental quality. This study aims to analyze (1) land-use change and urban sprawl work as determinants of environmental quality degradation in suburban areas. (2) The effect of urban sprawl, urban agglomeration, land-use change, urban activity systems, and transportation systems on environmental quality degradation in suburban areas. A combination of quantitative and qualitative approaches is used sequentially in this study. Data obtained through observation, surveys, and documentation. The results showed that the expansion of the Makassar City area to the suburbs had an impact on spatial dynamics, spatial segregation, and environmental degradation. Furthermore, urban sprawl, land-use change, urban agglomeration, activity systems, and transportation systems have a positive correlation to environmental quality degradation with a determination coefficient of 85.9%. This study recommends the handling of urban sprawl, land-use change, and urban agglomeration to be considered in the formulation of development policies towards the sustainability of natural resources and the environment of Makassar City, Indonesia.
Developments for Collagen Hydrolysate in Biological, Biochemical, and Biomedical Domains: A Comprehensive Review
Authors: Harris Muhammad , Potgieter Johan , Ishfaq Kashif , Shahzad Muhammad
Published: May 2021

The collagen hydrolysate, a proteinic biopeptide, is used for various key functionalities in humans and animals. Numerous reviews explained either individually or a few of following aspects: types, processes, properties, and applications. In the recent developments, various biological, biochemical, and biomedical functionalities are achieved in five aspects: process, type, species, disease, receptors. The receptors are rarely addressed in the past which are an essential stimulus to activate various biomedical and biological activities in the metabolic system of humans and animals. Furthermore, a systematic segregation of the recent developments regarding the five main aspects is not yet reported. This review presents various biological, biochemical, and biomedical functionalities achieved for each of the beforementioned five aspects using a systematic approach. The review proposes a novel three-level hierarchy that aims to associate a specific functionality to a particular aspect and its subcategory. The hierarchy also highlights various key research novelties in a categorical manner that will contribute to future research.
Stress Intensity Factors for Embedded, Surface, and Corner Cracks in Finite-Thickness Plates Subjected to Tensile Loading
Authors: Toribio Jesús , González Beatriz , Matos Juan-Carlos , Mulas Óscar
Published: May 2021

The aim of this study is to obtain the stress intensity factor (SIF) along the crack front of elliptical cracks located in finite-thickness plates subjected to imposed displacement or applied tensile load, for different crack geometries (relative depths and aspect ratios) and crack configurations (embedded, surface, and corner). The SIF was calculated from the J-integral, obtained by the finite element method. The results show how the SIF grows with the increase in the relative crack depth and with the decrease in the aspect ratio, with the corner crack being the most dangerous configuration and the embedded crack the most favorable configuration. By increasing the plate length, the SIF rises when the plate is under imposed displacement and decreases when the plate is subjected to applied tensile load, both cases tending towards the same SIF curve.
Graphene Oxide Topical Administration: Skin Permeability Studies
Authors: Silva Filipa A. L. S. , Costa-Almeida Raquel , Timochenco Licínia , Amaral Sara I. , Pinto Soraia , Gonçalves Inês C. , Fernandes José R. , Magalhães Fernão D. , Sarmento Bruno , Pinto Artur M.
Published: May 2021

Nanostructured carriers have been widely used in pharmaceutical formulations for dermatological treatment. They offer targeted drug delivery, sustained release, improved biostability, and low toxicity, usually presenting advantages over conventional formulations. Due to its large surface area, small size and photothermal properties, graphene oxide (GO) has the potential to be used for such applications. Nanographene oxide (GOn) presented average sizes of 197.6 ± 11.8 nm, and a surface charge of −39.4 ± 1.8 mV, being stable in water for over 6 months. 55.5% of the mass of GOn dispersion (at a concentration of 1000 µg mL−1) permeated the skin after 6 h of exposure. GOn dispersions have been shown to absorb near-infrared radiation, reaching temperatures up to 45.7 °C, within mild the photothermal therapy temperature range. Furthermore, GOn in amounts superior to those which could permeate the skin were shown not to affect human skin fibroblasts (HFF-1) morphology or viability, after 24 h of incubation. Due to its large size, no skin permeation was observed for graphite particles in aqueous dispersions stabilized with Pluronic P-123 (Gt–P-123). Altogether, for the first time, Gon’s potential as a topic administration agent and for delivery of photothermal therapy has been demonstrated.
Refractory Metal Coated Alumina Foams as Support Material for Stem Cell and Fibroblasts Cultivation
Authors: Hasemann Georg , Betke Ulf , Krüger Manja , Walles Heike , Scheffler Michael
Published: May 2021

Ceramics are widely used as implant materials; however, they are brittle and may emit particles when used in these applications. To overcome this disadvantage, alumina foams, which represent a 3D cellular structure comparable to that of human trabecular bone structures, were sputter coated with platinum, tantalum or titanium and modified with fibronectin or collagen type I, components of the extracellular matrix (ECM). To proof the cell material interaction, the unmodified and modified materials were cultured with (a) mesenchymal stem cells being a perfect indicator for biocompatibility and releasing important cytokines of the stem cell niche and (b) with fibroblasts characterized as mediators of inflammation and therefore an important cellular component of the foreign body reaction and inflammation after implantation. To optimize and compare the influence of metal surfaces on cellular behavior, planar glass substrates have been used. Identified biocompatible metal surface of platinum, titanium and tantalum were sputtered on ceramic foams modified with the above-mentioned ECM components to investigate cellular behavior in a 3D environment. The cellular alumina support was characterized with respect to its cellular/porous structure and niche accessibility and coating thickness of the refractory metals; the average cell size was 2.3 mm, the average size of the cell windows was 1.8 mm, and the total foam porosity was 91.4%. The Pt, Ti and Ta coatings were completely dense covering the entire alumina foam surface. The metals titanium and tantalum were colonized very well by the stem cells without a coating of ECM components, whereas the fibroblasts preferred components of the ECM on the alumina foam surface.
Degradation Monitoring of HDPE Material in CO2-Saturated NaCl Environment through Electrochemical Impedance Spectroscopy Technique
Authors: Khalid Hafiz Usman , Ismail Mokhtar Che , Nosbi Norlin
Published: May 2021

Extensive damage due to saturated seawater and CO2 exposure under high temperature and pressure in high-density polyethylene (HDPE) has been studied by Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), Thermogravimetric Analysis (TGA), Field Emission Scanning Electron Microscope (FESEM), and Electrochemical Impedance Spectroscopy (EIS). The degradation of square-shaped HDPE samples having 1 mm thickness was investigated at 70 bars with 60, 75, and 90 °C separately for three weeks in an autoclave chamber. A clear indication of aging was observed in terms of chain scission by the formation of the methyl group (1262 cm−1), and the appearance of degradation products, including the alcohol and hydroxyl groups. The decline in glass transition temperature (Tg), melting point (Tm), and crystallinity (Xc) result from branching and formation of degradation products in the aged samples. TGA results reveal that the degradation shifts the characteristic temperatures (T5% and T10%) to lower values compared to virgin HDPE. FESEM images show clear surface cracks and rough patches after 3 weeks. The Xc value increased due to chain mobility at higher temperatures (90 °C). The impedance is relatively high 1011 ohms.cm−2 for a virgin sample, but it drops down to 109 and 106 after degradation. Impedance and dielectric loss were correlated, and the significance of dielectric loss was observed at lower frequencies. These characterizations will contribute to more efficient and detailed evaluation criteria for degradation monitoring.
Oral Tissue Interactions and Cellular Response to Zirconia Implant-Prosthetic Components: A Critical Review
Authors: Kunrath Marcel F. , Gupta Saurabh , Lorusso Felice , Scarano Antonio , Noumbissi Sammy
Published: May 2021

Background: Dental components manufactured with zirconia (ZrO2) represent a significant percentage of the implant prosthetic market in dentistry. However, during the last few years, we have observed robust clinical and pre-clinical scientific investigations on zirconia both as a prosthetic and an implantable material. At the same time, we have witnessed consistent technical and manufacturing updates with regards to the applications of zirconia which appear to gradually clarify points which until recently were not well understood. Methods: This critical review evaluated the “state of the art” in relation to applications of this biomaterial in dental components and its interactions with oral tissues. Results: The physico-chemical and structural properties as well as the current surface treatment methodologies for ZrO2 were explored. A critical investigation of the cellular response to this biomaterial was completed and the clinical implications discussed. Finally, surface treatments of ZrO2 demonstrate that excellent osseointegration is possible and provide encouraging prospects for rapid bone adhesion. Furthermore, sophisticated surface treatment techniques and technologies are providing impressive oral soft tissue cell responses thus leading to superior biological seal. Conclusions: Dental devices manufactured from ZrO2 are structurally and chemically stable with biocompatibility levels allowing for safe and long-term function in the oral environment.
On S-Evolution Algebras and Their Enveloping Algebras
Authors: Mukhamedov Farrukh , Qaralleh Izzat
Published: May 2021

In the present paper, we introduce S-evolution algebras and investigate their solvability, simplicity, and semisimplicity. The structure of enveloping algebras has been carried out through the attached graph of S-evolution algebras. Moreover, we introduce the concept of E-linear derivation of S-evolution algebras, and prove such derivations can be extended to their enveloping algebras under certain conditions.
Enriching Elementary School Mathematical Learning with the Steepest Descent Algorithm
Authors: Araya Roberto
Published: May 2021

The steepest descent (or ascent) algorithm is one of the most widely used algorithms in Science, Technology, Engineering, and Mathematics (STEM). However, this powerful mathematical tool is neither taught nor even mentioned in K12 education. We study whether it is feasible for elementary school students to learn this algorithm, while also aligning with the standard school curriculum. We also look at whether it can be used to create enriching activities connected to children’s real-life experiences, thus enhancing the integration of STEM and fostering Computational Thinking. To address these questions, we conducted an empirical study in two phases. In the first phase, we tested the feasibility with teachers. In a face-to-face professional development workshop with 457 mathematics teachers actively participating using an online platform, we found that after a 10-min introduction they could successfully apply the algorithm and use it in a couple of models. They were also able to complete two complex and novel tasks: selecting models and adjusting the parameters of a model that uses the steepest descent algorithm. In a second phase, we tested the feasibility with 90 fourth graders from 3 low Socioeconomic Status (SES) schools. Using the same introduction and posing the same questions, we found that they were able to understand the algorithm and successfully complete the tasks on the online platform. Additionally, we found that close to 75% of the students completed the two complex modeling tasks and performed similarly to the teachers.
The Modigliani–Miller Theory with Arbitrary Frequency of Payment of Tax on Profit
Authors: Brusov Peter , Filatova Tatiana
Published: May 2021

The main purpose of the current study is the generalization and further development of the Modigliani–Miller theory taking into account one of the conditions of the real functioning of companies for the case of paying income tax with an arbitrary frequency (monthly, quarterly, semi-annual or annual payments). While a return is not required more than once a year, businesses may be responsible for filing estimated taxes based on profits earned. This requirement is dependent on showing a profit. For example, sole proprietors must file estimated taxes on profits quarterly, on the 15th day of April, June, September and January. In Russia, tax on profit payments could be made annually, quarterly, or monthly. We suppose, that more frequent payment of income tax impacts on all main financial indicators of the company and leads to some important consequences. We use analytical and numerical methods: we derive all main formulas of the modified Modigliani–Miller theory theoretically and then use them to obtain all main financial indicators of company and their dependences on different parameters by MS Excel. We show that: (1) all Modigliani–Miller theorems, statements and formulas change; (2) all main financial indicators, such as the weighted average cost of capital (WACC), company value, V, and equity cost, ke, depend on the frequency of tax on profit payments; (3) in the case of income tax payments more than once per year (at p≠1), as takes place in practice, the WACC, company value, V and equity cost, and ke start depend on debt cost, kd, while in ordinary (classical) Modigliani–Miller theory all these values do not depend on kd; (4) obtained results allow a company to choose the number of payments of tax on profit per year (of course, within actual tax legislation): more frequent payments of income tax are beneficial for both parties, for the company and for the tax regulator.
Generation of Virtual Patient Populations That Represent Real Type 1 Diabetes Cohorts
Authors: Ahmad Sayyar , Ramkissoon Charrise M. , Beneyto Aleix , Conget Ignacio , Giménez Marga , Vehi Josep
Published: May 2021

Preclinical testing and validation of therapeutic strategies developed for patients with type 1 diabetes (T1D) require a cohort of virtual patients (VPs). However, current simulators provide a limited number of VPs, lack real-life scenarios, and inadequately represent intra- and inter-day variability in insulin sensitivity and blood glucose (BG) profile. The generation of a realistic scenario was achieved by using the meal patterns, insulin profiles (basal and bolus), and exercise sessions estimated as disturbances using clinical data from a cohort of 14 T1D patients using the Medtronic 640G insulin pump provided by the Hospital Clínic de Barcelona. The UVa/Padova’s cohort of adult patients was used for the generation of a new cohort of VPs. Insulin model parameters were optimized and adjusted in a day-by-day fashion to replicate the clinical data to create a cohort of 75 VPs. All primary and secondary outcomes reflecting the BG profile of a T1D patient were analyzed and compared to the clinical data. The mean BG 166.3 versus 162.2 mg/dL (p = 0.19), coefficient of variation 32% versus 33% (p = 0.54), and percent of time in range (70 to 180 mg/dL) 59.6% versus 66.8% (p = 0.35) were achieved. The proposed methodology for generating a cohort of VPs is capable of mimicking the BG metrics of a real cohort of T1D patients from the Hospital Clínic de Barcelona. It can adopt the inter-day variations in the BG profile, similar to the observed clinical data, and thus provide a benchmark for preclinical testing of control techniques and therapy strategies for T1D patients.
Cardiac Rehabilitation and Complementary Physical Training in Elderly Patients after Acute Coronary Syndrome: A Pilot Study
Authors: Beigienė Aurelija , Petruševičienė Daiva , Barasaitė Vitalija , Kubilius Raimondas , Macijauskienė Jūratė
Published: May 2021

Background and Objectives: Nearly 23% of elderly patients hospitalized due to acute coronary syndrome have reduced muscle strength. It is assumed that these patients would better benefit from a complex training—a combination of endurance, strength, balance, coordination, and flexibility—in order to reduce the loss of muscle strength and mass and improve functional capacity. The aim of this study was to assess the effectiveness and safety of two different complementary resistance and balance training programs during short-term cardiac rehabilitation (CR) in elderly patients after a percutaneous or surgical intervention due to acute coronary syndrome. Materials and Methods: This randomized controlled trial was conducted from January 2020 to February 2021 in one Lithuanian rehabilitation hospital. A total of 63 participants who met the inclusion criteria were randomly assigned to three groups (at the ratio of 1:1:1): control (CG, n = 19), intervention 1 (IG-1, n = 26), and intervention 2 (IG-2, n = 18). All the patients attended a usual inpatient CR program of a mean duration of 18.7 ± 1.7 days, while the patients assigned to the intervention groups (IG-1 and IG-2) additionally received different resistance and balance training programs three days a week. Functional capacity, with 6-minute walk test (6MWT) and cardiopulmonary exercise testing (CPET), as well as physical performance, with the short physical performance battery (SPPB) test and one repetition maximum test (1RM) for leg press, were assessed at baseline and after CR. Results: The mean age of the participants was 72.9 ± 5.5 years; 73% were men. All parameters of functional capacity and physical performance improved significantly after CR (p < 0.05), except for peak VO2 that improved only in the IG-1. Comparison of CR effectiveness among the groups revealed no significant differences. Conclusions: All three rehabilitation programs were safe and well tolerated by elderly patients aged ≥65 years as well as improved functional capacity (6-minute walk distance and peak workload) and physical performance (SPPB and 1RM). Complementary resistance and balance training with traditional physical therapy means and exercises with mechanical devices did not show greater benefits for the results of physical performance compared with the usual CR program.
Multiphysics Modeling and Analysis of a Solar Desalination Process Based on Vacuum Membrane Distillation
Authors: Shuldes Benjamin N. , Bavarian Mona , Nejati Siamak
Published: May 2021

A hollow fiber vacuum membrane distillation (VMD) module was modeled using finite element analysis, and the results were used to conduct an exergy efficiency analysis for a solar-thermal desalination scheme. The performance of the VMD module was simulated under various operating conditions and membrane parameters. Membrane porosity, tortuosity, pore diameter, thickness, and fiber length were varied, along with feed temperature and feed configuration. In all cases, polarization phenomena were seen to inhibit the performance of the module. Under VMD operation, polarization of salt concentration was seen to be the main determining factor in the reduction of permeate flux. Within the boundary layer, salt concentration was seen to rapidly increase from the feed mass fraction of 0.035 to the saturation point. The increase in salt concentration led to a decrease in saturation pressure, the driving force for separation. Charging the feed into the shell instead of the lumen side of the membranes resulted in a further decrease in permeate flux. It is shown that adding a baffling scheme to the surface of the fibers can effectively reduce polarization phenomena and improve permeate flux. Increasing the overall recovery ratio was seen to increase the exergy efficiency of the system. Exergy efficiency was seen to have almost no dependency on membrane parameters due to the low recovery ratio in a single pass and the high heating duty required to reach the desired temperature for the feed stream.
The Impact of Simvastatin on Lipidomic Markers of Cardiovascular Risk in Human Liver Cells Is Secondary to the Modulation of Intracellular Cholesterol
Authors: Schooneveldt Yvette L. , Giles Corey , Keating Michael F. , Mellett Natalie A. , Jurrjens Aaron W. , Paul Sudip , Calkin Anna C. , Meikle Peter J.
Published: May 2021

Statins are the first-line lipid-lowering therapy for reducing cardiovascular disease (CVD) risk. A plasma lipid ratio of two phospholipids, PI(36:2) and PC(18:0_20:4), was previously identified to explain 58% of the relative CVD risk reduction associated with pravastatin, independent of a change in low-density lipoprotein-cholesterol. This ratio may be a potential biomarker for the treatment effect of statins; however, the underlying mechanisms linking this ratio to CVD risk remain unclear. In this study, we investigated the effect of altered cholesterol conditions on the lipidome of cultured human liver cells (Hep3B). Hep3B cells were treated with simvastatin (5 μM), cyclodextrin (20 mg/mL) or cholesterol-loaded cyclodextrin (20 mg/mL) for 48 h and their lipidomes were examined. Induction of a low-cholesterol environment via simvastatin or cyclodextrin was associated with elevated levels of lipids containing arachidonic acid and decreases in phosphatidylinositol species and the PI(36:2)/PC(18:0_20:4) ratio. Conversely, increasing cholesterol levels via cholesterol-loaded cyclodextrin resulted in reciprocal regulation of these lipid parameters. Expression of genes involved in cholesterol and fatty acid synthesis supported the lipidomics data. These findings demonstrate that the PI(36:2)/PC(18:0_20:4) ratio responds to changes in intracellular cholesterol abundance per se, likely through a flux of the n-6 fatty acid pathway and altered phosphatidylinositol synthesis. These findings support this ratio as a potential marker for CVD risk reduction and may be useful in monitoring treatment response.
Special Issue “Plant Viruses: From Ecology to Control”
Authors: Navas-Castillo Jesús , Fiallo-Olivé Elvira
Published: May 2021

Plant viruses cause many of the most important diseases threatening crops worldwide [...]
Stability of Atrazine–Smectite Intercalates: Density Functional Theory and Experimental Study
Authors: Moreno-Rodríguez Daniel , Jankovič Ľuboš , Scholtzová Eva , Tunega Daniel
Published: May 2021

Atrazine (A) is one of the most applied herbicides and has a negative impact on the environment and health. Density functional theory (DFT) and experimental methods were used in the study of the immobilization of atrazine in two smectites, montmorillonite (Mt) and beidellite (Bd), as well as in their organically modified structures. Four systems were examined: A-Mt and A-Bd, as well as the structures modified by tetramethylphosphonium cation (TMP), A-TMP-Mt and A-TMP-Bd. The calculations revealed a flat arrangement of the atrazine in the interlayer space of both smectites with higher stability of beidellite structures. The presence of the TMP cation increased the fixation of atrazine in both organically modified smectites. The calculated vibrational spectra allowed a detailed analysis of the overlapping bands observed in the experimental FTIR spectra and their correct assignment. Further, selected FTIR bands unambiguously assigned to atrazine and both smectites served for the estimation of the adsorbed amount of atrazine. It was shown that the adsorption capacity of both TMP-modified smectites did not increase in comparison to the adsorption capacity of unmodified smectite samples.
Mineralogy of Phoscorites of the Arbarastakh Complex (Republic of Sakha, Yakutia, Russia)
Authors: Kruk Mikhail Nikolaevich , Doroshkevich Anna Gennadievna , Prokopyev Ilya Romanovich , Izbrodin Ivan Aleksandrovich
Published: May 2021

The Arbarastakh ultramafic carbonatite complex is located in the southwestern part of the Siberian Craton and contains ore-bearing carbonatites and phoscorites with Zr-Nb-REE mineralization. Based on the modal composition, textural features, and chemical compositions of minerals, the phoscorites from Arbarastakh can be subdivided into two groups: FOS 1 and FOS 2. FOS 1 contains the primary minerals olivine, magnetite with isomorphic Ti impurities, phlogopite replaced by tetraferriphlogopite along the rims, and apatite poorly enriched in REE. Baddeleyite predominates among the accessory minerals in FOS 1. Zirconolite enriched with REE and Nb and pyrochlore are found in smaller quantities. FOS 2 has a similar mineral composition but contains much less olivine, magnetite is enriched in Mg, and the phlogopite is enriched in Ba and Al. Of the accessory minerals, pyrochlore predominates and is enriched in Ta, Th, and U; baddeleyite is subordinate and enriched in Nb. Chemical and textural differences suggest that the phoscorites were formed by the sequential introduction of different portions of the melt. The melt that formed the FOS 1 was enriched in Zr and REE relative to the FOS 2 melt; the melt that formed the FOS 2 was enriched in Al, Ba, Nb, Ta, Th, U, and, to a lesser extent, Sr.
Scale-Up Preparation of Crocins I and II from Gardeniajasminoides by a Two-Step Chromatographic Approach and Their Inhibitory Activity Against ATP Citrate Lyase
Authors: Guan Shuguang , Pu Qiaoli , Liu Yinan , Wu Honghong , Yu Wenbo , Pi Zifeng , Liu Shu , Song Fengrui , Li Jingya , Guo De-An
Published: May 2021

Crocins are highly valuable natural compounds for treating human disorders, and they are also high-end spices and colorants in the food industry. Due to the limitation of obtaining this type of highly polar compound, the commercial prices of crocins I and II are expensive. In this study, macroporous resin column chromatography combined with high-speed counter-current chromatography (HSCCC) was used to purify crocins I and II from natural sources. With only two chromatographic steps, both compounds were simultaneously isolated from the dry fruit of Gardenia jasminoides, which is a cheap herbal medicine distributed in a number of countries. In an effort to shorten the isolation time and reduce solvent usage, forward and reverse rotations were successively utilized in the HSCCC isolation procedure. Crocins I and II were simultaneously obtained from a herbal resource with high recoveries of 0.5% and 0.1%, respectively, and high purities of 98.7% and 99.1%, respectively, by HPLC analysis. The optimized preparation method was proven to be highly efficient, convenient, and cost-effective. Crocins I and II exhibited inhibitory activity against ATP citrate lyase, and their IC50 values were determined to be 36.3 ± 6.24 and 29.7 ± 7.41 μM, respectively.
Bioprocessing of Marine Chitinous Wastes for the Production of Bioactive Prodigiosin
Authors: Nguyen Thi-Hanh , Wang San-Lang , Nguyen Dai-Nam , Nguyen Anh-Dzung , Nguyen Thi-Huyen , Doan Manh-Dung , Ngo Van-Anh , Doan Chien-Thang , Kuo Yao-Haur , Nguyen Van-Bon
Published: May 2021

Recently, microbial prodigiosin (PG) has received much attention due to its numerous beneficial applications. The aim of this study was to establish the bioprocessing of marine chitinous wastes (MCWs) for the cost-effective preparation of PG. Of the MCWs, demineralized shrimp shell powders (de-SSP) were found to be a potential source of carbon/nitrogen (C/N) for PG production by bacterial fermentation using Serratia marcescens strains. Further, PG scale-up production was investigated in a 15 L bioreactor system, and the highest yield (6200 mg/L) was achieved during fermentation using 5 L of a novel-designed culture broth that included 1.60% C/N sources (a de-SSP/casein ratio of 7/3), 0.02% K2SO4, and 0.05% K2HPO4, with an initial pH of 6–7. Fermentation was conducted in the dark at 27.5 °C for 8.0 h. This study was the first to report on the utilization of shrimp wastes for cost-effective, large-scale (5 L/pilot) PG production with high productivity (6200 mg/L) in a short cultivation time. The combination of 0.02% K2SO4 and 0.05% K2HPO4 was also found to be a novel salt composition that significantly enhanced PG yield. The red compound was purified and confirmed as PG after analyzing its HPLC profile, mass, and UV/vis spectra. The purified PG was then tested for its bioactivities and showed effective anticancer activities, moderated antioxidant activities, and novel anti-NO effects.
Thermoelectric Materials for Textile Applications
Authors: Chatterjee Kony , Ghosh Tushar K.
Published: May 2021

Since prehistoric times, textiles have served an important role–providing necessary protection and comfort. Recently, the rise of electronic textiles (e-textiles) as part of the larger efforts to develop smart textiles, has paved the way for enhancing textile functionalities including sensing, energy harvesting, and active heating and cooling. Recent attention has focused on the integration of thermoelectric (TE) functionalities into textiles—making fabrics capable of either converting body heating into electricity (Seebeck effect) or conversely using electricity to provide next-to-skin heating/cooling (Peltier effect). Various TE materials have been explored, classified broadly into (i) inorganic, (ii) organic, and (iii) hybrid organic-inorganic. TE figure-of-merit (ZT) is commonly used to correlate Seebeck coefficient, electrical and thermal conductivity. For textiles, it is important to think of appropriate materials not just in terms of ZT, but also whether they are flexible, conformable, and easily processable. Commercial TEs usually compromise rigid, sometimes toxic, inorganic materials such as bismuth and lead. For textiles, organic and hybrid TE materials are more appropriate. Carbon-based TE materials have been especially attractive since graphene and carbon nanotubes have excellent transport properties with easy modifications to create TE materials with high ZT and textile compatibility. This review focuses on flexible TE materials and their integration into textiles.
Black Cumin (Nigella sativa L.): A Comprehensive Review on Phytochemistry, Health Benefits, Molecular Pharmacology, and Safety
Authors: Hannan Md. Abdul , Rahman Md. Ataur , Sohag Abdullah Al Mamun , Uddin Md. Jamal , Dash Raju , Sikder Mahmudul Hasan , Rahman Md. Saidur , Timalsina Binod , Munni Yeasmin Akter , Sarker Partha Protim , Alam Mahboob , Mohibbullah Md. , Haque Md. Nazmul , Jahan Israt , Hossain Md. Tahmeed , Afrin Tania , Rahman Md. Mahbubur , Tahjib-Ul-Arif Md. , Mitra Sarmistha , Oktaviani Diyah Fatimah , Khan Md Kawsar , Choi Ho Jin , Moon Il Soo , Kim Bonglee
Published: May 2021

Mounting evidence support the potential benefits of functional foods or nutraceuticals for human health and diseases. Black cumin (Nigella sativa L.), a highly valued nutraceutical herb with a wide array of health benefits, has attracted growing interest from health-conscious individuals, the scientific community, and pharmaceutical industries. The pleiotropic pharmacological effects of black cumin, and its main bioactive component thymoquinone (TQ), have been manifested by their ability to attenuate oxidative stress and inflammation, and to promote immunity, cell survival, and energy metabolism, which underlie diverse health benefits, including protection against metabolic, cardiovascular, digestive, hepatic, renal, respiratory, reproductive, and neurological disorders, cancer, and so on. Furthermore, black cumin acts as an antidote, mitigating various toxicities and drug-induced side effects. Despite significant advances in pharmacological benefits, this miracle herb and its active components are still far from their clinical application. This review begins with highlighting the research trends in black cumin and revisiting phytochemical profiles. Subsequently, pharmacological attributes and health benefits of black cumin and TQ are critically reviewed. We overview molecular pharmacology to gain insight into the underlying mechanism of health benefits. Issues related to pharmacokinetic herb–drug interactions, drug delivery, and safety are also addressed. Identifying knowledge gaps, our current effort will direct future research to advance potential applications of black cumin and TQ in health and diseases.
Consumption of Breast Milk Is Associated with Decreased Prevalence of Autism in Fragile X Syndrome
Authors: Westmark Cara J.
Published: May 2021

Breastfeeding is associated with numerous health benefits, but early life nutrition has not been specifically studied in the neurodevelopmental disorder fragile X syndrome (FXS). Herein, I evaluate associations between the consumption of breast milk during infancy and the prevalence of autism, allergies, diabetes, gastrointestinal (GI) problems and seizures in FXS. The study design was a retrospective survey of families enrolled in the Fragile X Online Registry and Accessible Research Database (FORWARD). There was a 1.7-fold reduction in the prevalence of autism in FXS participants who were fed breast milk for 12 months or longer. There were strong negative correlations between increased time the infant was fed breast milk and the prevalence of autism and seizures and moderate negative correlations with the prevalence of GI problems and allergies. However, participants reporting GI problems or allergies commenced these comorbidities significantly earlier than those not fed breast milk. Parsing the data by sex indicated that males exclusively fed breast milk exhibited decreased prevalence of GI problems and allergies. These data suggest that long-term or exclusive use of breast milk is associated with reduced prevalence of key comorbidities in FXS, although breast milk is associated with the earlier development of GI problems and allergies.
Dietary Diversity and Healthy Aging: A Prospective Study
Authors: Zhang Jian , Zhao Ai
Published: May 2021

Population aging is a global phenomenon. The present study determined the effects of dietary diversity score (DDS) and food consumption on healthy aging. A subset of the data of the China Health and Nutrition Survey was utilized in this study. DDSs were calculated using the dietary data collected in the years 2009 and 2011. A healthy aging score (HAS) was calculated by summing the standardized scores on physical functional limitation, comorbidity, cognitive function, and psychological stress based on the data collected in the year 2015, with a lower HAS indicating a healthier aging process. Life quality was self-reported in the year 2015. This study found that DDS was inversely associated with HAS (T3 vs. T1: β −0.16, 95%CI −0.20 to −0.11, p-trend <0.001). The consumption of meat and poultry, aquatic products, and fruits was inversely associated with HAS, and participants in the highest tertile of staple foods consumption had a higher HAS than those in the lowest tertile. HAS was inversely associated with good self-reported life quality and positively associated with bad life quality. In conclusion, food consumption may influence the aging process, and adherence to a diverse diet is associated with a healthier aging process in elderly people.
Hepatic Nfe2l2 Is Not an Essential Mediator of the Metabolic Phenotype Produced by Dietary Methionine Restriction
Authors: Fang Han , Stone Kirsten P. , Ghosh Sujoy , Forney Laura A. , Sims Landon C. , Vincik LeighAnn , Gettys Thomas W.
Published: May 2021

The principal sensing of dietary methionine restriction (MR) occurs in the liver, where it activates multiple transcriptional programs that mediate various biological components of the response. Hepatic Fgf21 is a key target and essential endocrine mediator of the metabolic phenotype produced by dietary MR. The transcription factor, Nfe2l2, is also activated by MR and functions in tandem with hepatic Atf4 to transactivate multiple, antioxidative components of the integrated stress response. However, it is unclear whether the transcriptional responses linked to Nfe2l2 activation by dietary MR are essential to the biological efficacy of the diet. Using mice with liver-specific deletion of Nfe2l2 (Nfe2l2fl/(Alb)) and their floxed littermates (Nfe2l2fl/fl) fed either Control or MR diets, the absence of hepatic Nfe2l2 had no effect on the ability of the MR diet to increase FGF21, reduce body weight and adiposity, and increase energy expenditure. Moreover, the primary elements of the hepatic transcriptome were similarly affected by MR in both genotypes, with the only major differences occurring in induction of the P450-associated drug metabolism pathway and the pentose glucuronate interconversion pathway. The biological significance of these pathways is uncertain but we conclude that hepatic Nfe2l2 is not essential in mediating the metabolic effects of dietary MR.
Depression and Vegetarians: Association between Dietary Vitamin B6, B12 and Folate Intake and Global and Subcortical Brain Volumes
Authors: Berkins Samuel , Schiöth Helgi Birgir , Rukh Gull
Published: May 2021

Deficiency of vitamin B6 and vitamin B12, mostly in vegetarians, is found to be associated with depression and adverse neurological function. We investigated whether vitamin B6, B12, and folate have an effect on brain structure, especially among depressed people who follow a specific diet. The study sample comprised 9426 participants from the UK Biobank cohort with a mean age of 62.4 years. A generalized linear model controlling for age, sex, body mass index, ethnicity, town send deprivation index, educational qualification, smoking, and alcohol intake was used to test the association between study groups and structural brain volumes. Depression was more prevalent, and intake of vitamin B6 and B12 was lower among vegetarians, while non-vegetarians had a lower intake of folate. Overall, no significant association was observed between vitamin B6, B12, and folate intakes and both global and subcortical brain volumes among participants with depression. However, vitamin B12 intake was positively associated with right pallidum among non-depressed participants, and a significant interaction between vitamin B12 intake and depression status on the right pallidum was observed. Also, a significant interaction between folate intake and depression status on grey matter (GM) volume and left thalamus was observed. Upon diet stratification, folate intake is associated with total brain volume and GM volume among vegetarians with depression. Furthermore, no significant associations were observed for subcortical regions. Our findings suggest that dietary intake of vitamin B6 and B12 might have an effect on brain structure. Vegetarians, particularly those who suffer from depression may benefit from supplementing their diets with vitamins B6, B12, and folate to ensure brain health. Further studies, especially with a larger sample size and longitudinal design, are needed to confirm these findings.
The Potential Link between Episodes of Diverticulitis or Hemorrhoidal Proctitis and Diets with Selected Plant Foods: A Case–Control Study
Authors: Flich-Carbonell Juan , Alegre-Martinez Antoni , Alfonso-Sanchez Jose L. , Torres-Sanchez Maria T. , Gomez-Abril Segundo , Martínez-Martínez Maria I. , Martin-Moreno José M.
Published: May 2021

Diverticulitis and hemorrhoidal proctitis in the population are significant public health problems. We studied the potential association between the intake of certain plant foods and diverticulitis or hemorrhoidal episodes through a case–control study including 410 cases and 401 controls. We used a semiquantitative food frequency questionnaire. The intake was additionally quantified according to a 24 h recall. The plant foods or derived food products were categorized by their main chemical components into ethanol, caffeine/theine/theobromine, capsaicin, alliin, acids, eugenol, and miscellaneous foods such as curcumin. The mean score for overall intake of plant foods under consideration was 6.3 points, and this was significantly higher in cases (8.5) than in controls (4.1). Overall intake was similar in cases presenting with diverticulitis or hemorrhoidal proctitis. Cases had 13 times the odds of being in the upper quartile for overall intake (>7 points), compared to controls. Explanatory logistic regression models showed that the strongest association with diverticulitis and hemorrhoidal proctitis was shown by the chemical food group of capsaicin, followed by ethanol, eugenol, caffeine/theine/theobromine, and acids. Neither alliin nor miscellaneous food groups showed any association. High, frequent consumption of capsaicin, followed by ethanol, eugenol, caffeine/theine/theobromine, and acids increase the risk of diverticulitis and hemorrhoidal proctitis.
First Autochthonous West Nile Lineage 2 and Usutu Virus Infections in Humans, July to October 2018, Czech Republic
Authors: Zelená Hana , Kleinerová Jana , Šikutová Silvie , Straková Petra , Kocourková Hana , Stebel Roman , Husa Petr , Husa Petr , Tesařová Eva , Lejdarová Hana , Šebesta Oldřich , Juráš Peter , Ciupek Renata , Mrázek Jakub , Rudolf Ivo
Published: May 2021

We present epidemiological, clinical and laboratory findings of five Czech patients diagnosed with autochthonous mosquito-borne disease—four patients with confirmed West Nile virus (WNV) and one patient with Usutu virus (USUV) infections, from July to October 2018, including one fatal case due to WNV. This is the first documented human outbreak caused by WNV lineage 2 in the Czech Republic and the first record of a neuroinvasive human disease caused by USUV, which illustrates the simultaneous circulation of WNV and USUV in the country.
GO-Y078, a Curcumin Analog, Induces Both Apoptotic Pathways in Human Osteosarcoma Cells via Activation of JNK and p38 Signaling
Authors: Lu Peace Wun-Ang , Lin Renn-Chia , Yang Jia-Sin , Lu Eric Wun-Hao , Hsieh Yi-Hsien , Tsai Meng-Ying , Lu Ko-Hsiu , Yang Shun-Fa
Published: May 2021

Osteosarcoma is the most common primary bone malignancy in teenagers and continues to confer a generally poor prognosis due to its highly metastatic potential. Poor solubility in water and instability of curcumin limits its bioavailability for use in the adjuvant situation to improve the prognosis and the long-term survival of patients with osteosarcoma. To further obtain information regarding the apoptosis induced by a new curcumin analog, GO-Y078, in human osteosarcoma cells, flow cytometric analysis, annexin V-FITC/PI apoptosis staining assay, human apoptosis array, and Western blotting were employed. GO-Y078 dose-dependently decreased viabilities of human osteosarcoma U2OS, MG-63, 143B, and Saos-2 cells and induced sub-G1 fraction arrest and apoptosis in U2OS and 143B cells. In addition to the effector caspase 3 and poly adenosine diphosphate-ribose polymerase, GO-Y078 significantly activated both initiators of extrinsic caspase 8 and intrinsic caspase 9, whereas cellular inhibitors of apoptosis 1 (cIAP-1) and X-chromosome-linked IAP (XIAP) in U2OS and 143B cells were significantly repressed. Moreover, GO-Y078 increased phosphorylation of extracellular signal-regulated protein kinases (ERK)1/2, c-Jun N-terminal kinases (JNK)1/2, and p38 in U2OS and 143B cells. Using inhibitors of JNK (JNK-in-8) and p38 (SB203580), GO-Y078′s increases in cleaved caspases 8, 9, and 3 could be expectedly suppressed, but they could not be affected by co-treatment with the ERK inhibitor (U0126). Altogether, GO-Y078 simultaneously induces both apoptotic pathways and cell arrest in U2OS and 143B cells through activating JNK and p38 signaling and repressing IAPs. These findings contribute to a better understanding of the mechanisms responsible for GO-Y078′s apoptotic effects on human osteosarcoma cells.