bluuebunny/crossref_metadata_2025 huggingface dataset:
Prevalence, Risk Factors, and Clinical Correlates of Insomnia in China College Student During the COVID-19
Authors: Yu Chang , Li Xingxing , Qi Gangqiao , Yang Liang , Fu Wenbo , Yao Qin , Wei Lei , Zhou Dongsheng , Zhang Xiangyang , Zheng Hong
Published: August 2021

Objective: The 2019 novel coronavirus disease (COVID-19) broke out in Hubei Province and spread rapidly to the whole country, causing huge public health problems. College students are a special group, and there is no survey on insomnia among college students. The purpose of this study was to investigate the incidence and related factors of insomnia in college students during the period of COVID-19.

Method: A total of 1,086 college students conducted a cross-sectional study through the questionnaire star platform. The survey time was from February 15 to February 22, 2020. The collected information included demographic informatics and mental health scale, Athens Insomnia Scale (AIS) to assess sleep quality, Self-Reporting Questionnaire-20 (SRQ-20) to assess general psychological symptoms, Chinese perceived stress scale (CPSS) to assess stress. We used logistic regression to analyze the correlation between related factors and insomnia symptoms.

Results: The prevalence of insomnia, general psychological symptoms and stress were 16.67, 5.8, and 40.70%, respectively. Multivariate logistic regression analysis showed that gender (OR = 1.55, p = 0.044, 95% CI = 1.00–2.41), general psychological symptoms (OR = 1.49, p < 0.01, 95% CI = 1.40–1.60) and living in an isolation unit (OR = 2.21, p = 0.014, 95% CI = 1.17–4.16) were risk factors for insomnia of college students.

Conclusion: Our results show that the insomnia is very common among college students during the outbreak of covid-19, and the related factors include gender, general psychological symptoms and isolation environment. It is necessary to intervene the insomnia of college students and warrants attention for mental well-being of college students.
Resilience as a Mediator of the Association Between Perceived Stigma and Quality of Life Among People With Inflammatory Bowel Disease
Authors: Luo Dan , Zhou Meijing , Sun Lifu , Lin Zheng , Bian Qiugui , Liu Meihong , Ren Shurong
Published: August 2021

Background: Improving Quality of Life (QOL) is an essential objective in the management of inflammatory bowel disease. An accumulating body of research has been conducted to explore the association between perceived stigma and QOL among patients with chronic illness. Still, underlying mechanisms behind this pathway have not been thoroughly examined.

Objective: To investigate (a) the effect of perceived stigma on QOL among patients with inflammatory bowel disease; and (b) the mediating role of resilience in the association between perceived stigma and QOL.

Methods: This cross-sectional study included a convenient sample of patients diagnosed with inflammatory bowel disease from four tertiary hospitals in Jiangsu Province, China. Patients completed the Perceived Stigma Scale in Inflammatory Bowel Disease (PSS-IBD), the Resilience Scale for Patients with Inflammatory Bowel Disease (RS-IBD), and the Inflammatory Bowel Disease Questionnaire (IBDQ). A bootstrapping analysis was implemented using the SPSS macro PROCESS.

Results: A total of 311 patients with Cohn's disease and ulcerative colitis participated in this study, and 57.6% were men. The mean disease duration was 3.51 ± 1.04 years. Approximately 40% of the sample exceeded the criterion score for moderate stigma. Patients who perceived moderate or severe stigma reported lower QOL compared with those with mild stigma. After controlling for sociodemographic and clinical variables, we observed that perceived stigma was negatively associated with resilience. Moreover, resilience was found to mediate the relationship between perceived stigma and all aspects of QOL.

Conclusions: These findings suggested that QOL of patients with inflammatory bowel disease was associated with perceived stigma and resilience and identified the mediating effects of resilience in the relationship between perceived stigma and QOL. Furthermore, this suggests that integrating intervention techniques to target resilience into the QOL improvement program of individuals with perceived stigma is possible.
Leptin Gene and Leptin Receptor Gene Polymorphisms in Alcohol Use Disorder: Findings Related to Psychopathology
Authors: Browning Brittney D. , Schwandt Melanie L. , Farokhnia Mehdi , Deschaine Sara L. , Hodgkinson Colin A. , Leggio Lorenzo
Published: August 2021

Comorbidity between alcohol use disorder (AUD) and other addictive and psychiatric disorders is highly prevalent and disabling; however, the underlying biological correlates are not fully understood. Leptin is a peptide hormone known for its role in energy homeostasis and food intake. Furthermore, leptin plays a key role in the activity of the hypothalamic-pituitary-adrenal (HPA) axis and of several neurotransmitter systems that regulate emotionality and behavior. However, human studies that have investigated circulating leptin levels in relation to AUD and affective disorders, such as anxiety and depression, are conflicting. Genetic-based analyses of the leptin gene (LEP) and leptin receptor gene (LEPR) have the potential of providing more insight into the potential role of the leptin system in AUD and comorbid psychopathology. The aim of the current study was to investigate whether genotypic variations at LEP and LEPR are associated with measures of alcohol use, nicotine use, anxiety, and depression, all of which represent common comorbidities with AUD. Haplotype association analyses were performed, using data from participants enrolled in screening and natural history protocols at the National Institute on Alcohol Abuse and Alcoholism (NIAAA). Analyses were performed separately in European Americans and African Americans due to the variation in haplotype diversity for most genes between these groups. In the European American group, one LEP haplotype (EB2H4) was associated with lower odds of having a current AUD diagnosis, two LEPR haplotypes (EB7H3, EB8H3) were associated with lower cigarette pack years and two LEPR haplotypes (EB7H2, EB8H2) were associated with higher State-Trait Anxiety Inventory (STAI-T) scores. In the African American group, one LEP haplotype (AB2H8) was associated with higher cigarette pack years and one LEP haplotype (AB3H2) was associated with lower Fagerström Test for Nicotine Dependence (FTND) scores. Overall, this study found that variations in the leptin and leptin receptor genes are associated with measures of alcohol use, nicotine use, and anxiety. While this preliminary study adds support for a role of the leptin system in AUD and psychopathologies, additional studies are required to fully understand the underlying mechanisms and potential therapeutic implications of these findings.
COVID-19 Vaccination in Lower-Middle Income Countries: National Stakeholder Views on Challenges, Barriers, and Potential Solutions
Authors: Tagoe Eunice Twumwaa , Sheikh Nurnabi , Morton Alec , Nonvignon Justice , Sarker Abdur Razzaque , Williams Lynn , Megiddo Itamar
Published: August 2021

The development of COVID-19 vaccines does not imply the end of the global pandemic as now countries have to purchase enough COVID-19 vaccine doses and work towards their successful rollout. Vaccination across the world has progressed slowly in all, but a few high-income countries (HICs) as governments learn how to vaccinate their entire populations amidst a pandemic. Most low- and middle-income countries (LMICs) have been relying on the COVID-19 Vaccines Global Access (COVAX) Facility to obtain vaccines. COVAX aims to provide these countries with enough doses to vaccinate 20% of their populations. LMICs will likely encounter additional barriers and challenges rolling out vaccines compared HICs despite their significant experience from the Expanded Programme on Immunisation (EPI). This study explores potential barriers that will arise during the COVID-19 vaccine rollout in lower-middle-income countries and how to overcome them. We conducted sixteen semi-structured interviews with national-level stakeholders from Ghana and Bangladesh (eight in each country). Stakeholders included policymakers and immunisation programme experts. Data were analysed using a Framework Analysis technique. Stakeholders believed their country could use existing EPI structures for the COVID-19 vaccine rollout despite existing challenges with the EPI and despite its focus on childhood immunisation rather than vaccinating the entire population over a short period of time. Stakeholders suggested increasing confidence in the vaccine through community influencers and by utilising local government accredited institutions such as the Drug Authorities for vaccine approval. Additional strategies they discussed included training more health providers and recruiting volunteers to increase vaccination speed, expanding government budgets for COVID-19 vaccine purchase and delivery, and exploring other financing opportunities to address in-country vaccine shortages. Stakeholders also believed that LMICs may encounter challenges complying with priority lists. Our findings suggest that COVID-19 vaccination is different from previous vaccination programs, and therefore, policymakers have to expand the EPI structure and also take a systematic and collaborative approach to plan and effectively rollout the vaccines.
Impact of the National Health Insurance Coverage Policy on the Utilisation and Accessibility of Innovative Anti-cancer Medicines in China: An Interrupted Time-Series Study
Authors: Fang Wenqing , Xu Xinglu , Zhu Yulei , Dai Huizhen , Shang Linlin , Li Xin
Published: August 2021

Objective: The study aimed to evaluate the impact of the National Health Insurance Coverage (NHIC) policy on the utilisation and accessibility of innovative anti-cancer medicines in Nanjing, China.

Methods: We used the adjusted World Health Organisation and Health Action International methodology to calculate the price and availability of 15 innovative anti-cancer medicines included in the National Health Insurance drug list in 20 tertiary hospitals and six secondary hospitals in Nanjing before and after NHIC policy implementation. Interrupted time-series regression was used to analyse the changes in the utilisation of the study medicines.

Results: The price reduction rates of innovative anti-cancer medicines ranged between 34 and 65%. The mean availability rate was 27.44% before policy implementation and increased to 47.33% after policy implementation. The utilisation of anti-cancer medicines suddenly increased with a slope of 33.19–2,628.39 when the policy was implemented. Moreover, the usage rate of bevacizumab, bortezomib, and apatinib significantly increased (p < 0.001, p = 0.009, and p < 0.001, respectively) after policy implementation. With regard to price reduction and medical insurance reimbursement, the medicines became more affordable after policy implementation (0.06–1.90 times the per capita annual disposable income for urban patients and 0.13–4.46 times the per capita annual disposable income for rural patients).

Conclusion: The NHIC policy, which was released by the central government, effectively improved the utilisation and affordability of innovative anti-cancer medicines. However, the availability of innovative anti-cancer medicines in hospitals remained low and the utilisation of innovative anti-cancer medicines was affected by some factors, including the incidence of cancer, limitation of indications within the insurance program, and the rational use of innovative anti-cancer medicines. It is necessary to improve relevant supporting policies to promote the affordability of patients. The government should speed up the process of price negotiation to include more innovative anti-cancer medicines in the medical insurance coverage, consider including both medical examinations and adjuvant chemotherapy in the medical insurance, and increase investment in health care.
Awareness Mentality and Strategic Behavior in Science
Authors: Ball Rafael
Published: August 2021

Acknowledgement of scientific achievements was and is essentially achieved through the citation of a publication. Increasingly, however, it is no longer just the publication itself that plays an important role, but also the degree of attention that a scientist achieves with this very publication. Thus, the importance of strategic behavior in science is progressing and an awareness mentality is spreading. In this paper, the causes and backgrounds of this development are discussed, identifying the use of reductionist, quantitative systems in science management and research funding, the loss of critical judgment and technocratic dominance, quantitative assessments used for decision making, altmetrics and the like as alternative views, the use of perception scores in reference databases and universities as well as ambitions of journals as main drivers. Besides, different forms of strategic behavior in science and the resulting consequences and impacts are being highlighted.
Luteal Phase Support Using Subcutaneous Progesterone: A Systematic Review
Authors: Conforti Alessandro , Carbone Luigi , Iorio Giuseppe Gabriele , Cariati Federica , Bagnulo Francesca , Marrone Vincenzo , Strina Ida , Alviggi Carlo
Published: August 2021

Luteal phase support (LPS) is crucial in assisted reproductive technology (ART) cycles when the luteal phase has been found to be defective. Such deficiency is most likely related to the supraphysiological steroid levels that usually occurr in stimulated cycles which, in turn, could severely affect luteinizing hormone (LH) secretion and function, thereby negatively influencing the luteal phase. A number of different medications and routes have been successfully used for LPS in ART. Although an optimal protocol has not yet been identified, the existing plethora of medications offer the opportunity to personalize LPS according to individual needs. Subcutaneous administration progesterone has been proposed for LPS and could represent an alternative to a vaginal and intramuscular route. The aim of the present systematic review is to summarize the evidence found in the literature concerning the application of subcutaneous progesterone in ARTs, highlighting the benefits and limits of this novel strategy. With this aim in mind, we carried out systematic research in the Medline, ISI Web of Knowledge, and Embase databases from their inception through to November 2020. Randomized controlled trials (RCTs) were preferred by the authors in the elaboration of this article, although case-control and cohort studies have also been considered. According to our findings, evidence exists which supports that, in women with a good prognosis undergoing a freshin vitrofertilization (IVF) cycle, subcutaneous Pg is not inferior to vaginal products. In the Frozen-thawed embryo transfer (FET) cycle, data concerning efficacy is mixed with an increased miscarriage rate in women undergoing a subcutaneous route in oocyte donor recipients. Data concerning the acceptance of the subcutaneous route versus the vaginal route are encouraging despite the different scales and questionnaires which were used. In addition, a cost-effective analysis has not yet been conducted.
Co-creation Pathways to Inform Shared Governance of Urban Living Labs in Practice: Lessons From Three European Projects
Authors: Mahmoud Israa Hanafi , Morello Eugenio , Ludlow David , Salvia Giuseppe
Published: August 2021

Over recent decades Urban Living Labs (ULLs) have become a common space for co-creation processes' experimentation, whereby new approaches for sustainable urban development are highly connected to support evidence-based policy generation. Europe seems a particular 'hotspot' for this approach whenever it comes to public policy and specifically planning for urban transition. Systemic changes related to urban governance and different public participatory mechanisms, as in the case of ULLs, demand a growing interest from the stakeholders and deliberation in decision-making mechanisms. In this research paper, we analyze co-creation pathways and different shared governance mechanisms in three ongoing European projects: CLEVER Cities, Sharing Cities, and SUNEX projects from a practice perspective. This comparative study investigates stakeholder engagement (1) scales, (2) mechanisms, (3) methodologies of engagement, and finally the co-creation pathway challenges and pitfalls. From the analyzed ULLs' experiences, we identified key principles that suggest relevant clues to enable the consolidation of a forthcoming ULL 2.0 model and related innovation pathways for co-creating urban planning policies. We lastly reflect on the enablers and catalysts of co-creation processes to inform shared urban governance as major takeaways from our research.
Affective Visualization in Virtual Reality: An Integrative Review
Authors: Pinilla Andres , Garcia Jaime , Raffe William , Voigt-Antons Jan-Niklas , Spang Robert P. , Möller Sebastian
Published: August 2021

A cluster of research in Affective Computing suggests that it is possible to infer some characteristics of users’ affective states by analyzing their electrophysiological activity in real-time. However, it is not clear how to use the information extracted from electrophysiological signals to create visual representations of the affective states of Virtual Reality (VR) users. Visualization of users’ affective states in VR can lead to biofeedback therapies for mental health care. Understanding how to visualize affective states in VR requires an interdisciplinary approach that integrates psychology, electrophysiology, and audio-visual design. Therefore, this review aims to integrate previous studies from these fields to understand how to develop virtual environments that can automatically create visual representations of users’ affective states. The manuscript addresses this challenge in four sections: First, theories related to emotion and affect are summarized. Second, evidence suggesting that visual and sound cues tend to be associated with affective states are discussed. Third, some of the available methods for assessing affect are described. The fourth and final section contains five practical considerations for the development of virtual reality environments for affect visualization.
Enchanted by Your Surrounding? Measuring the Effects of Immersion and Design of Virtual Environments on Decision-Making
Authors: Oberdörfer Sebastian , Heidrich David , Birnstiel Sandra , Latoschik Marc Erich
Published: August 2021

Impaired decision-making leads to the inability to distinguish between advantageous and disadvantageous choices. The impairment of a person’s decision-making is a common goal of gambling games. Given the recent trend of gambling using immersive Virtual Reality it is crucial to investigate the effects of both immersion and the virtual environment (VE) on decision-making. In a novel user study, we measured decision-making using three virtual versions of the Iowa Gambling Task (IGT). The versions differed with regard to the degree of immersion and design of the virtual environment. While emotions affect decision-making, we further measured the positive and negative affect of participants. A higher visual angle on a stimulus leads to an increased emotional response. Thus, we kept the visual angle on the Iowa Gambling Task the same between our conditions. Our results revealed no significant impact of immersion or the VE on the IGT. We further found no significant difference between the conditions with regard to positive and negative affect. This suggests that neither the medium used nor the design of the VE causes an impairment of decision-making. However, in combination with a recent study, we provide first evidence that a higher visual angle on the IGT leads to an effect of impairment.
The Effect of Deficit Irrigation Strategies on the Efficiency From Plant to Essential Oil Production in Peppermint (Mentha piperita L.)
Authors: Akbarzadeh Ali , Shahnazari Ali
Published: August 2021

The effect of deficit irrigation on water productivity in the water supply and utilization chain has been computed, employing a systematic and quantitative approach. By applying such an investigation, weaknesses and strengths of deficit irrigation strategies could be revealed, and actions and measures could be implemented to improve water productivity as much as possible. The peppermint plants were subjected to regulated deficit irrigation (RDI) and partial root-zone drying (PRD). Peppermint was cultivated under full irrigation (FI, control) and RDI treatments including RDI85, RDI70, RDI55, and RDI40, receiving 85, 70, 55, and 40% of FI treatment, respectively; PRD techniques including PRD70, PRD55, and PRD40, receiving 70, 55, and 40% of FI treatment in one side of the root-zone at each irrigation event, respectively during two cutting seasons. There was no significant improvement in leaves weight and dried yield step by applying deficit irrigation treatments. Leaves and aerial parts from the whole plant and dried yield as the main source of essential oil had the weakest function, which should be optimized to adjust and gain more efficiency. In the essential oil yield step, as a final procedure, the highest improvements were observed in PRD55 treatment with 90% improvement in efficiency compared to control treatment. According to the results and calculating water productivity, PRD55 treatment improved water productivity by 179% compared to the control treatment. Two-way ANOVA analysis, between deficit volume and the deficit applying method, showed that total water productivity was affected (increased) significantly by the deficit applying method. This implies the contribution of the PRD technique to enhance more signals than RDI-based signals, which resulted in more improvements in secondary metabolism production in peppermint.
Quantification of Workload and Wellness Measures in a Women's Collegiate Volleyball Season
Authors: Kupperman Natalie , Curtis Michael A. , Saliba Susan A. , Hertel Jay
Published: August 2021

The purpose of this paper was to quantify internal and external loads completed by collegiate volleyball athletes during a competitive season. Eleven players were sampled (using accelerometers and subjective wellness surveys) during the practice (n= 55) and game (n= 30) sessions over the 2019 season. Longitudinal data were evaluated for trends across the preseason, non-conference play, and conference play periods. Data were also analyzed with respect to positional groups. Longitudinal analysis of accelerometer data showed higher workload demand during practices than games. Positional group differences were most when evaluating jump count and height. Setters accrued over twice as many jumps in a practice than during a game and had similar overall jump counts in practice to attacking positions. Average team wellness values varied with time in the season, especially during times of congested travel. This is the first study to look at both game and practice workload and wellness measures in collegiate women's volleyball. The results suggest athlete monitoring can be used to understand the demands of volleyball and used in the future to enhance practice and recovery day design to optimize athlete well-being.
Virtual Strategies for the Broad Delivery of High Intensity Exercise in Persons With Spinal Cord Injury: Ongoing Studies and Considerations for Implementation
Authors: McMillan David W. , Astorino Todd A. , Correa Michael A. , Nash Mark S. , Gater David R.
Published: August 2021

Spinal cord injury (SCI) results in a multitude of metabolic co-morbidities that can be managed by exercise. As in the non-injured population, manipulation of exercise intensity likely allows for fruitful optimization of exercise interventions targeting metabolic health in persons with SCI. In this population, interventions employing circuit resistance training (CRT) exhibit significant improvements in outcomes including cardiorespiratory fitness, muscular strength, and blood lipids, and recent exploration of high intensity interval training (HIIT) suggests the potential of this strategy to enhance health and fitness. However, the neurological consequences of SCI result in safety considerations and constrain exercise approaches, resulting in the need for specialized exercise practitioners. Furthermore, transportation challenges, inaccessibility of exercise facilities, and other barriers limit the translation of high intensity “real world” exercise strategies. Delivering exercise via online (“virtual”) platforms overcomes certain access barriers while allowing for broad distribution of high intensity exercise despite the limited number of population-specific exercise specialists. In this review, we initially discuss the need for “real world” high intensity exercise strategies in persons with SCI. We then consider the advantages and logistics of using virtual platforms to broadly deliver high intensity exercise in this population. Safety and risk mitigation are considered first followed by identifying strategies and technologies for delivery and monitoring of virtual high intensity exercise. Throughout the review, we discuss approaches from previous and ongoing trials and conclude by giving considerations for future efforts in this area.
Hyaluronic Acid Is an Effective Dermal Filler for Lip Augmentation: A Meta-Analysis
Authors: Czumbel László Márk , Farkasdi Sándor , Gede Noémi , Mikó Alexandra , Csupor Dezső , Lukács Anita , Gaál Valéria , Kiss Szabolcs , Hegyi Péter , Varga Gábor
Published: August 2021

Introduction:The lips and the mouth play an indispensable role in vocalization, mastication and face aesthetics. Various noxious factors may alter and destruct the original structure, and appearance of the lips and the anatomical area surrounding the mouth. The application of hyaluronic acid (HA) may serve as a safe method for lip regeneration. Although a number of studies exist for HA effectiveness and safety, its beneficial effect is not well-established.

Aim:The present meta-analysis and systematic review was performed to investigate the effectiveness of HA on lip augmentation. We also investigated the types and nature of adverse effects (AEs) of HA application.

Methods:We reported our meta-analysis in accordance with the PRISMA Statement. PROSPERO protocol registration: CRD42018102899. We performed the systematic literature search in CENTRAL, Embase, and MEDLINE. Randomized controlled trials, cohort studies, case series and case reports were included. The untransformed proportion (random-effects, DerSimonian-Laird method) of responder rate to HA injection was calculated. For treatment related AEs descriptive statistics were used.

Results:The systematic literature search yielded 32 eligible records for descriptive statistics and 10 records for quantitative synthesis. The results indicated that the overall estimate of responders (percentage of subjects with increased lip fullness by one point or higher) was 91% (ES = 0.91, 95% CI:0.85−0.96) 2 months after injection. The rate of responders was 74% (ES = 0.74, 95% CI:0.66−0.82) and 46% (ES = 0.46, 95% CI:0.28−0.65) after 6 and 12 months, respectively. We included 1,496 participants for estimating the event rates of AEs. The most frequent treatment-related AEs were tenderness (88.8%), injection site swelling (74.3%) and bruising (39.5%). Rare AEs included foreign body granulomas (0.6%), herpes labialis (0.6%) and angioedema (0.3%).

Conclusion:Our meta-analysis revealed that lip augmentation with injectable HA is an efficient method for increasing lip fullness for at least up to 6 months after augmentation. Moreover, we found that most AEs of HA treatment were mild or moderate, but a small number of serious adverse effects were also found. In conclusion, further well-designed RCTs are still needed to make the presently available evidence stronger.
The Competitiveness of Beef Exports From Burkina Faso to Ghana
Authors: Rich Karl M. , Wane Abdrahmane
Published: August 2021

Despite large volumes of cattle stocks in the Sahel, most exports of cattle products remain as live animal sales rather than meat. However, there is increased interest amongst donors and governments to increase value-added exports of beef. In this paper, we provide results from a simulation analysis that explores the prospective competitiveness and benefits of exporting beef from Burkina Faso to Ghana rather than live animals. The paper reviews trading patterns in live animals along the corridor and meat imports from overseas destinations to Ghana. Model results highlight limited competitiveness of the main products demanded in destination markets (offals). Market segmentation strategies, infrastructure development, and animal productivity all generate marginal improvements in competitiveness, but not enough to compete with third-country supplies. Only specific, largely external macroeconomic conditions provide for significant improvements in competitiveness. The paper further reveals the relatively modest employment gains associated with increased exports of meat in lieu of live animals. The analysis suggests a re-think on large-scale investments in downstream functions in the value chain, instead illustrating the fundamental role of upstream investments in productivity, animal health, and collective action to promote greater market integration between pastoralists and formal sector buyers.
Quantitative Expression of TYR, CD34, and CALD1 Discriminates Between Canine Oral Malignant Melanomas and Soft Tissue Sarcomas
Authors: Tsoi Mayra F. , Thaiwong Tuddow , Smedley Rebecca C. , Noland Erica , Kiupel Matti
Published: August 2021

Canine oral malignant melanomas (OMMs) exhibit a variety of morphologic phenotypes, including a spindloid variant. The microscopic diagnosis of spindloid OMMs is based on junctional activity and/or the presence of melanin pigment. In the absence of these features, spindloid OMMs are difficult to differentiate from soft tissue sarcomas (STS). An antibody cocktail (MDX) that includes Melan-A, PNL2, and tyrosinase-related proteins 1 and 2 (TRP-1 and TRP-2) is the current gold standard for identifying amelanotic OMMs by immunohistochemistry (IHC). However, MDX is less sensitive for diagnosing spindloid amelanotic OMMs. This raises concern for biopsy specimens that lack overlying epithelium, making it potentially difficult to differentiate OMM from STS by IHC. The goal of this study was to identify additional markers to help differentiate between STS and OMMs that lack pigment and junctional activity. SOX-10 has recently been proposed as a sensitive marker for melanocytes in humans but has not been validated in dogs. Similarly, RNA expression for various genes has been analyzed in humans, but not in the context of diagnosing canine melanocytic neoplasms. For this retrospective study, formalin-fixed, paraffin-embedded tissues from 20 OMMs, 20 STS, and 20 oral spindle cell tumors (OSCTs) that lacked junctional activity and pigmentation were selected. IHC for MDX, SOX-10, and laminin, in parallel with RT-qPCR ofTYR, SOX10, CALD1, CD34, DES, andLAMA1, was performed in all cases.TYR, CD34, andCALD1were the most discriminatory genes in differentiating between OMM and STS, all having 100% specificity and 65, 95, and 60% sensitivity, respectively. While all 20 OMMs were immunohistochemically labeled for SOX-10, two STS were also labeled (100% sensitivity and 90% specificity). MDX IHC labeled all 20 OMMs and no STS. Surprisingly, none of the 20 OSCTs expressedTYRRNA above the cutoff, and 14/20 OSCTs expressedCALD1orCD34RNA above the cutoff, thereby confirming them as STS. Four OSCT were suspect STS, and no OSCTs were confirmed as OMMs based on IHC and RNA expression patterns. In conclusion, the RNA levels of TYR, CD34, and CALD1 should be evaluated in suspected amelanotic OMMs that are negative for MDX to accurately differentiate between OMM and STS.
Burden and Determinants of Chronic Kidney Disease Among Diabetic Patients in Ethiopia: A Systematic Review and Meta-Analysis
Authors: Tolossa Tadesse , Fetensa Getahun , Regassa Bikila , Yilma Mekdes Tigistu , Besho Merga , Fekadu Ginenus , Wakuma Bizuneh , Bekele Daniel , Mulisa Diriba
Published: August 2021

Background: Chronic kidney disease (CKD) among diabetic patients is becoming a global health burden with a high economic cost to health systems. The incidence of CKD is higher in low-income countries such as Ethiopia. In Ethiopia, there is no national representative evidence on the burden and determinants of CKD among patients with diabetes. Therefore, this review aimed to estimates the pooled burden and determinants of CKD among patients with diabetes.

Methods: Published articles from various electronic databases such as Pub Med, Google Scholar, CINAHL, Scopes, Cochrane library, the Web of Science, and African Journals Online were accessed. Also, unpublished studies from Addis Ababa digital library were identified. We included all observational studies (cross-sectional, case-control, and cohort) in the review. Data were extracted on the Microsoft Excel spreadsheet and analyzed using STATA 14.1 version. A random-effects model was used to estimate the pooled estimate with a 95% confidence interval (CI). Forest plots were used to visualize the presence of heterogeneity and estimate the pooled burden and determinants of chronic kidney disease among diabetic patients. The presence of publication bias was assessed by funnel plots and Egger’s statistical tests.

Results: Published (297) and unpublished (2) literature were identified from several databases and digital libraries, of which twelve articles were selected for final meta-analysis. Significant heterogeneity was observed across studies (I2 = 85.2%), which suggests a random-effects model to estimate pooled burden. The analysis found that the pooled burden of CKD among patients with diabetes was 18.22% (95% CI: 15.07–21.38). Factors such as hypertension (OR = 2.65, 95%, CI: 1.38, 5.09), type of DM (OR = 0.33, 95%, CI: 0.14–0.76), and duration of DM (OR = 0.51, 95%, CI: 0.34–0.77) were found to have significant association with CKD.

Conclusion: The current review revealed a higher burden of CKD among patients with diabetes in Ethiopia. The presence of hypertension, type II diabetes, and duration of diabetes for a longer duration were found to be independent determinants of CKD among patients with diabetes. For better control of chronic kidney disease, integrated management of hypertension and DM should be designed with a special focus on chronic diabetic patients.
Design and Characterization of a Planar Motor Drive Platform Based on Piezoelectric Hemispherical Shell Resonators
Authors: Schiele Frank , Gundelsweiler Bernd
Published: August 2021

In this study, a planar ultrasonic motor platform is presented that uses three half-side excited piezoelectric hemispherical shell resonators. To understand the working principle and the harmonic vibration behavior of the piezoelectric resonator, the trajectory of the friction contact was measured in free-oscillating mode at varying excitation frequencies and voltages. The driving performance of the platform was characterized with transport loads up to 5 kg that also serve as an influencing downforce for the friction motor. The working range for various transport loads and electrical voltages up to 30 V is presented. Undesirable noise and parasitic oscillations occur above the detected excitation voltage ranges, depending on the downforce. Therefore, minimum and maximum values of the excitation voltage are reported, in which the propulsion force and the speed of the planar motor can be adjusted, and noiseless motion applies. The multidimensional driving capacity of the platform is demonstrated in two orthogonal axes and one rotary axis in open-loop driving mode, by measuring forces and velocities to confirm its suitability as a planar motor concept. The maximum measured propulsion force of the motor was 7 N with a transport load of 5 kg, and its maximum measured velocity was 77 mm/s with a transport load of 3 kg.
Plunging Airfoil: Reynolds Number and Angle of Attack Effects
Authors: Camacho Emanuel A. R. , Neves Fernando M. S. P. , Silva André R. R. , Barata Jorge M. M.
Published: August 2021

Natural flight has consistently been the wellspring of many creative minds, yet recreating the propulsive systems of natural flyers is quite hard and challenging. Regarding propulsive systems design, biomimetics offers a wide variety of solutions that can be applied at low Reynolds numbers, achieving high performance and maneuverability systems. The main goal of the current work is to computationally investigate the thrust-power intricacies while operating at different Reynolds numbers, reduced frequencies, nondimensional amplitudes, and mean angles of attack of the oscillatory motion of a NACA0012 airfoil. Simulations are performed utilizing a RANS (Reynolds Averaged Navier-Stokes) approach for a Reynolds number between 8.5×103 and 3.4×104, reduced frequencies within 1 and 5, and Strouhal numbers from 0.1 to 0.4. The influence of the mean angle-of-attack is also studied in the range of 0∘ to 10∘. The outcomes show ideal operational conditions for the diverse Reynolds numbers, and results regarding thrust-power correlations and the influence of the mean angle-of-attack on the aerodynamic coefficients and the propulsive efficiency are widely explored.
Riparian Zone Nitrogen Management through the Development of the Riparian Ecosystem Management Model (REMM) in a Formerly Glaciated Watershed of the US Northeast
Authors: Tamanna Marzia , Pradhanang Soni M. , Gold Arthur J. , Addy Kelly , Vidon Philippe G.
Published: August 2021

The Riparian Ecosystem Management Model (REMM) was developed, calibrated and validated for both hydrologic and water quality data for eight riparian buffers located in a formerly glaciated watershed (upper Pawcatuck River Watershed, Rhode Island) of the US Northeast. The Annualized AGricultural Non-Point Source model (AnnAGNPS) was used to predict the runoff and sediment loading to the riparian buffer. Overall, results showed REMM simulated water table depths (WTDs) and groundwater NO3-N concentrations at the stream edge (Zone 1) in good agreement with measured values. The model evaluation statistics showed that, hydrologically REMM performed better for site 1, site 4, and site 8 among the eight buffers, whereas REMM simulated better groundwater NO3-N concentrations in the case of site 1, site 5, and site 7 when compared to the other five sites. The interquartile range of mean absolute error for WTDs was 3.5 cm for both the calibration and validation periods. In the case of NO3-N concentrations prediction, the interquartile range of the root mean square error was 0.25 mg/L and 0.69 mg/L for the calibration and validation periods, respectively, whereas the interquartile range of d for NO3-N concentrations was 0.20 and 0.48 for the calibration and validation period, respectively. Moreover, REMM estimation of % N-removal from Zone 3 to Zone 1 was 19.7%, and 19.8% of N against actual measured 19.1%, and 26.6% of N at site 7 and site 8, respectively. The sensitivity analyses showed that changes in the volumetric water content between field capacity and saturation (soil porosity) were driving water table and denitrification.
Evaluation of Prognostic Factors, including Duodenal P-Glycoprotein Expression, in Canine Chronic Enteropathy
Authors: Pietra Marco , Galiazzo Giorgia , Bresciani Francesca , Morini Maria , Licarini Sara , Turba Maria Elena , Amaducci Gianmaria , Bettini Giuliano , Fracassi Federico , Ostanello Fabio
Published: August 2021

The aim of this retrospective single-center study was to evaluate which factors, including expression of P-glycoprotein (P-gp), a membrane-bound protein involved in multiple drug resistance, could predict the response to treatment in canine immunosuppressant-responsive enteropathy (IRE). Dogs with IRE or non-responsive enteropathy (NRE) that were examined from 2005 to 2014 were included and were divided into two groups (IRE vs. NRE). Signalment, history, and clinical and laboratory findings were collected. P-glycoprotein immunohistochemistry was carried out on duodenal biopsies of both groups stored in our biobank, and immunophenotyping and molecular clonality were performed on the NRE samples. Ninety-two dogs were enrolled, 73 IRE (79.3%) and 19 NRE (20.7%), with a prevalence of pure breed (78.3% vs. 21.7%) and male dogs (p < 0.001). Factors associated with a worse prognosis were previous treatment with steroids (p = 0.033) and lower serum total protein concentration (p = 0.005). Clonality testing on the NRE duodenal biopsies showed 5/16 clonal responses, assuming a latent undiagnosed lymphoma as a possible cause of the NRE.
High Drug Resistance in Feline Mammary Carcinoma Cell Line (FMCm) and Comparison with Human Breast Cancer Cell Line (MCF-7)
Authors: Correia Ana Salomé , Matos Rita , Gärtner Fátima , Amorim Irina , Vale Nuno
Published: August 2021

Drug repurposing and drug combination are important therapeutic approaches in cancer therapy. Drug repurposing aims to give new indications to drugs, rather than the original indication, whereas drug combination presupposes that the effect that is obtained should be more beneficial than the effect obtained by the individual drugs. Previously, drug repurposing and the combination of different drugs was evaluated in our research group against human breast cancer cells (MCF-7 cells). Our results demonstrated that the response obtained through the combination of drugs, when compared with the single drugs, led to more synergic responses. Therefore, using potential drugs for repurposing, combined with a reference drug in breast cancer (5-Fluorouracil), was the major aim of this project, but for the first time using the feline mammary carcinoma cell line, FMCm. Surprisingly, the feline neoplastic cells demonstrated considerable resistance to the drugs tested in isolation, and the combination was not effective, which contrasted with the obtained MCF-7 cells’ response.
A Water-Soluble Antibiotic in Rhubarb Stalk Shows an Unusual Pattern of Multiple Zones of Inhibition and Preferentially Kills Slow-Growing Bacteria
Authors: Bhattacharjee Mrinal K. , Bommareddy Praveen K. , DePass Anthony L.
Published: August 2021

Organic extract of Rhubarb (Rheum officinale) roots is known to have several medicinal uses. However, not much research has been done with the rhubarb stalk. The aim of this research is to evaluate the anti-bacterial and anti-proliferative effects of the aqueous extract from rhubarb stalks. The crude aqueous extract was further purified using anion exchange and gel filtration. The purified compound demonstrated broad spectrum antibacterial activity against the Gram-negative bacteria, E. coli and Aggregatibacter actinomycetemcomitans, and Gram-positive bacteria, S. aureus. A time-kill assay demonstrated that the antibiotic has strong bactericidal activity. It also has anti-proliferative action against the breast cancer cell line MCF-7 with no cytotoxicity, although the crude extract had a significant cytotoxic effect. The antibiotic activity, as measured by the diameter of the zone of inhibition, increased by several fold in low nutrient and/or low salt agar, suggesting that the antibiotic preferentially kills slow-growing bacteria. The antibiotic also gives an unusual pattern of multiple zones of inhibition in which several zones of cell growth are seen within the zone of inhibition. In conclusion, the active component in the aqueous extract of rhubarb stalk has great potential as a strong bactericidal antibiotic and as an anti-proliferative drug.
Exogenous Antioxidants Impact on UV-Induced Changes in Membrane Phospholipids and the Effectiveness of the Endocannabinoid System in Human Skin Cells
Authors: Gęgotek Agnieszka , Jastrząb Anna , Dobrzyńska Marta , Biernacki Michał , Skrzydlewska Elżbieta
Published: August 2021

Natural antioxidants effectively counteract changes caused by UV radiation in human skin cells. However, their action is limited due to their lipo/hydrophilicity. Therefore, the aim of this study was to analyze the mutual protective action of hydrophilic ascorbic acid and partially lipophilic rutin against UVA/UVB-induced changes in membranes phospholipid and endocannabinoid system in keratinocytes and fibroblasts. Obtained results clearly showed that, despite the stronger antioxidant properties of ascorbic acid, the lipid membranes were more effectively protected against UV-induced oxidation by rutin, including changes in phospholipid fatty acid levels, prevention against reactive aldehydes formation and endocannabinoids degradation. Ascorbic acid more strongly prevented UV-induced endocannabinoid receptors expression in fibroblasts, especially CB1. However, the combined action of used antioxidants resulted in the greatest cytoprotective effect, which was evident in the inflammatory marker TNFα down-regulation and increased cell viability following cell irradiation. The applied mixture of antioxidants showed a stronger protective in relation to membrane phospholipids in keratinocytes and in the endocannabinoid system in fibroblasts. In conclusion, it can be suggested that combined antioxidant capacities of ascorbic acid and rutin protects against lipid peroxidation but also decreases the UV-induced inflammation by direct interaction with the endocannabinoid system, thus increasing skin cell viability.
Volume and Distribution of Periprosthetic Bone Cysts in the Distal Tibia and Talus before Early Revision of Total Ankle Arthroplasty
Authors: Kim Seoyeong , Jang Jinju , Choi Jae-Hyuk , Yang Hai-Mi , Chun Heoung-Jae , Lee Gun-Woo , Lee Keun-Bae , Lim Dohyung
Published: August 2021

Periprosthetic osteolysis is a common complication following total ankle arthroplasty (TAA). However, understanding of osteolysis volume and distribution is still evolving, undermining efforts to reduce the incidence of osteolysis via bone remodeling. We obtained data on the characteristics of osteolysis developing within the distal tibia and talus after TAA. Three-dimensional computed tomography (3D-CT) reconstructions of 12 patients who underwent HINTEGRA TAA were performed. We identified 27 volumes of interest (VOIs) in the tibia and talus and used statistical methods to identify the characteristics of osteolysis in the VOIs. The osteolysis volume was significantly larger in the talus than in the tibia (162.1 ± 13.6 and 54.9 ± 6.1 mm3, respectively, p = 0.00). The extent of osteolysis within the peri-prosthetic region was greater than within other regions (p < 0.05). Particularly, in the talus, the region around the talar pegs exhibited 24.2 ± 4.5% more osteolysis than any other talar region (p = 0.00). Our results may suggest that extensive osteolysis within the peri-prosthetic region reflects changes in stress flow and distribution, which vary according to the design and placement of the fixation components. This is the first study to report 3D osteolysis patterns after TAA. Careful planning of TAA design improvements may reduce the incidence of osteolysis. Our results will facilitate the further development of TAA systems.
Review of Microsoft HoloLens Applications over the Past Five Years
Authors: Park Sebeom , Bokijonov Shokhrukh , Choi Yosoon
Published: August 2021

Since Microsoft HoloLens first appeared in 2016, HoloLens has been used in various industries, over the past five years. This study aims to review academic papers on the applications of HoloLens in several industries. A review was performed to summarize the results of 44 papers (dated between January 2016 and December 2020) and to outline the research trends of applying HoloLens to different industries. This study determined that HoloLens is employed in medical and surgical aids and systems, medical education and simulation, industrial engineering, architecture, civil engineering and other engineering fields. The findings of this study contribute towards classifying the current uses of HoloLens in various industries and identifying the types of visualization techniques and functions.