bluuebunny/crossref_metadata_2025 huggingface dataset:
Konsep Diri LANSIA di Panti Jompo
Authors: Yentika Yiyit
Published: December 2019

When someone getting old, he/she will get physical changing and physical decreasing which is influencing self-concept decreasing. From the result of introduction study in Tresna Werdha Sabai Nan Aluih Sicincin, the researchers get data as 110 oldsters male and female. Based on the interview to these three elders that has been nursed in this dwelling, one elder says happy although living far away from the family and the brother feels grateful, 2 elders say that they feel sad and yearning of their family. The elders describe their selves that they are old, sick often, and have low self esteem, shy, and feeling inferior. Living in the dwelling is like being thrown away and do not have any interactions anymore.

 
Pendidikan Kejuruan di Era Industri 4.0: Tantangan dan Peluang Karier
Authors: Maysitoh Maysitoh , Agung Desri Fitri , Afdal Afdal
Published: December 2019

Pendidikan kejuruan adalah salah satu sarana yang pengembangan ketrampilan siswa agar siap kerja. Perubahan dunia saat ini yang juga tengah memasuki era revolusi industri 4.0 semakin memberikan tantangan bagi individu untuk mampu menunjukkan eksistensinya pada era ini. Konseling karier yang terintegritas dengan pendidikan memegang kontribusi penting dalam membantu individu dalam hal ini. Adanya konseling karir dinilai menjadikan individu lebih matang dalam merencanakan karier dan meminimalisir stress kerja.Tulisan ini bertujuan memaparkan tantangan serta peluang karier pada pendidikan kejuruan di era industri 4.0. Kajian ini menggunakan metode kepustakaan (library research), sedangkan  pengumpulan data dilakukan dengan menelaah dan/atau mengekplorasi beberapa jurnal, buku, hasil penelitian, dan dokumen-dokumen (baik yang berbentuk cetak maupun elektronik) serta sumber-sumber data dan atau informasi lainnya yang dianggap relevan dengan kajian.
Menumbuh kembangkan Kreativitas Dalam Meningkatkan Prestasi Belajar Siswa
Authors: Syahri Lia Mita
Published: December 2019

Kreativitas, siswa, dunia pendidikan, prestasi belajar memiliki hubungan yang sangat erat, dalam hal ini objek yang paling utama yaitu siswa tersebut, dimana siswa dapat mencapai hasil prestasi yang membanggakan dengan berbagai unsur atau factor salah satunya dapat berupa pengungkapan diri kreativitas pada siswa tersebut. Jika di kaji maka kreativitas itu dapat dikatakan sebagai pemecahan masalah bagi siswa dalam hal pembelajaran sesuai dengan kemampuan dan potensi yang dimiliki oleh siswa. Banyak hal yang dapat dilakukan oleh siswa dalam mewujudkan kreativitas dalam mencapai hasil prestasi belajar siswa, dalam hal-hal kecil pun bisa dilakukan oleh siswa, seperti penggunaan catatan pelajaran, penghafalan rumus dengan cara yang disesuaikan dengan tipe belajar siswa, penggunaan waktu belajar dan aktivitas yang bisa membuat efektif antara waktu belajar dan aktivitas lainnya. Kreativitas yang dapat dilakukan oleh siswa dapat berdampak dengan hasil belajar siswa yang membanggakan, sehingga dapat dikatakan bahwa siswa itu memiliki prestasi dalam belajar, dengan bukti siswa mendapatkan nilai yang memuaskan yang sesuai dengan proses pembelajaran selama dilakukan oleh siswa. Peroleha nilai atau tindakan dan sikap siswa yang baik memang merupakan bukti bahwa prestasi tersebut telah dicapai oleh siswa, maka dari itu kreativitas memiliki peranan dalam hal prestasi belajar.  Sedangkan dalam segi belajar, siswa dapat dikatakan memiliki prestasi apabila sikap dan tingkah lakunya itu terjadi perubahan yang maju atau baik dari sebelumnya, maka dari itu apa yang dinamakan belajar dapat dikatakan berhasil.
Use of content mastery service using role playing approach improve student's emotional intelligence
Authors: Putri Meirizka Liyani , Taufik Taufik , Yendi Frischa Meivilona , Yuca Verlanda
Published: December 2019

Emotional intelligence is the ability possessed by individuals to be able to recognize, understand, manage and manage emotions and understand the emotions of others. There are many problems that arise related to emotional intelligence, especially in the school environment. To be able to overcome this and increase the emotional intelligence of BK teacher students can carry out content mastery services that aim to help students develop and learn new aspects or competencies related to emotional intelligence. Implementation of content mastery services can also be done with a role playing approach by playing the role associated with increasing emotional intelligence.
Kehidupan narapidana di LAPAS (Lembaga Pemasyarakatan)
Authors: Sum Egin Elga Dean , Veronika Monika , Pilosusan Sofia
Published: December 2019

LAPAS life is far from pleasant, all have rules and cannot do anything with looks. Because things that can make prisoners become frustrated and stressed. This is usually also caused by those who use them that cause them. Life experienced by prisoners is very limited and they must do anything that has been canceled by LAPAS
PENGADAAN USAHA WEDANG UWUH DENGAN PEMBERDAYAAN IBU RUMAH TANGGA SEBAGAI PENGENTAS KEMISKINAN
Authors: Hartati Anies Siti , Suryaningsum Sri
Published: December 2019

This is an action research conducted with empowerment effort which is addressed to housewife in Dusun Dukuh Imogiri Bantul. The data collection used in this research is observation method, document and interview. This study aims to improve the knowledge and skills of housewives. Efforts to empower done by considering the surrounding natural resources that have not been optimally utilized herbs that thrive in the yard of the community. The empowerment effort is done by training activity, that is the training of wedang uwuh making and marketing training of wedang uwuh. In addition to improving knowledge and skills as well as providing alternative productive activities, this training is also an effort to eradicate poverty because the sale of wedang uwuh can improve the economy of Dusun Dukuh.
PENGEMBANGAN LINGKUNGAN BELAJAR BLENDED LEARNING BERBASIS LEARNING MANAGEMENT SYSTEM BAGI MTs. MUHAMMADIYAH DI KABUPATEN SOLOK
Authors: Hanesman Hanesman , Adri Muhammad , Dewi Ika Parma , Jaya Putra
Published: December 2019

Industrial Revolution 4.0 has provided changes in various aspects of social life, one it’s changed in the Education sector which called by Education 4.0, utilizing information and digital technology as a supporting technology on the educational process, both in administration and learning processes. This effort to adopt this technology into education is also carried out by private education institutions, including MTs. Muhammadiyah (MTs.M) in Solok Regency, which is part of the junior secondary education.  As a private educational institution, MTs.M realizes it is very urgent to increase the competitiveness and the quality of its education, which one of it effort is an integration of technology in the learning process called Blended Learning (BL) through the development of Learning Management systems (LMS) used in online learning management. The experimental method is used to see the improvement of teacher knowledge and skills in LMS-based BL which begins with a survey of school readiness and teacher competencies in BL, then continues with the LMS-based BL training activities for 30 teachers from 9 MTs.M. Pre and Post Training Questionnaire design is used in data collection. The results of this research data show an increase in teacher competence in LMS-based BL, with high enthusiasm in developing LMS as a learning system in each MTs.M.
AUGMENTED REALITY SEBAGAI MEDIA PEMBELAJARAN INOVATIF DI ERA REVOLUSI INDUSTRI 4.0
Authors: Hendriyani Yeka , Effendi Hansi , Novaliendry Dony , Effendi Hastria
Published: December 2019

The main purpose of this paper is to uncover the results of a survey study regarding teacher opinions on the use of Augmented Reality (AR) in learning. For this purpose, marker-based cellular augmented reality applications have been developed and computer hardware is used as training material. In addition, teachers are given training for four days to get to know the basics of augmented reality technology, the software used, the environment to create marker-based AR objects. The data was collected through surveys and open-ended questions on teachers who are members of the English MGMP Kab. 50 cities. The tools and technology needed to develop AR and development experience are also shared. According to the survey results teachers are very enthusiastic about using augmented reality in their learning. The result of this article is the perception of the use of Augmented Reality technology for the development of instructional media by English teachers in the positive category.
Fortalecimiento organizacional de asociaciones de productores de café en las provincias de Loja y Zamora Chinchipe, Ecuador
Authors: Espinoza Loayza Viviana
Published: December 2019

En concordancia con los objetivos de desarrollo sostenible 8 y 4 y el Plan Nacional del Buen Vivir se ejecutó el proyecto “Fortalecimiento organizacional asociaciones de la economía popular y solidaria en las provincias de Loja y Zamora Chinchipe” el cual tiene como fin contribuir al fortalecimiento de las capacidades administrativas, financieras, contables y legales de las asociaciones productoras de café ubicadas en las provincias de Loja y Zamora Chinchipe. El proyecto vinculó a seis asociaciones, cuatro de ellas pertenecientes a la Federación Regional de Asociaciones de Pequeños Cafetaleros Ecológicos del Sur (FAPECAFES). Se trabajó con el fin de fortalecer la asociatividad de los productores y comercializadores de café a través de la aplicación y desarrollo de habilidades administrativas, contables-financieras y legales; para ello a partir de la metodología de investigación-acción se aplican herramientas de los temas antes mencionados, con la finalidad de desarrollar y gestionar instrumentos e información necesaria para mejorar la toma de decisiones. Entre los principales resultados obtenidos, se dispone de un diagnóstico detallado de cada asociación, insumo base para el proceso de planificación y ejecución del proceso de asistencia técnica y capacitación organizacional. Se cuenta con asociaciones capacitadas en temas legales, financieros, contables y administrativos; dicha capacitación reposa en manuales elaborados por alumnos y docentes, así como material audiovisual, esto con el fin de dotar a las asociaciones de material de consulta permanente, como soporte para su gestión.
Vinculación Universidad – Emprendimiento Socio Productivo de Ecuador.
Authors: Ramón Sandra
Published: December 2019

En Ecuador la principal fuente de empleo son los emprendimientos socio productivos, creados de manera empírica, organizados entre varios miembros de la comunidad y carentes de estructura y cultura empresarial. Ante esta realidad, la Universidad Técnica Particular de Loja ha emprendido en la ejecución de proyectos de vinculación con la sociedad, con la participación de los estudiantes universitarios de las titulaciones de Contabilidad, Administración de Empresas, Hotelería, Banca y Finanzas, para el efecto se aplicó la metodología de aprendizaje basado en proyectos que permitió un rol activo y participativo del estudiante quien analiza, se vincula y aplica los conocimientos adquiridos en su formación. La vinculación realizada permitió llegar a 209 emprendimientos socio productivos ubicados en Pichincha, Azuay, Guayas, El Oro y Loja principalmente. El 78,95% cuentan con más de 10 socios, 84,69% poseen registro único del contribuyente, 81,34% son apoyados por instituciones del estado, 51,20% superan los 4 años de funcionamiento, 53,11% se encuentran liderados por mujeres de 30 a 59 años. Los principales logros alcanzados son, emprendedores conscientes de la importancia de organizarse, trabajar en equipo, establecer procesos, utilizar herramientas administrativas y visualizarse ante la ciudadanía mediante el uso de redes sociales y la participación de eventos empresariales. Una vez desarrollado el proyecto se concluye que existe apertura para que los estudiantes universitarios apliquen los conocimientos administrativos y planteen estructuras, estrategias a corto y largo plazo para el fortalecimiento empresarial. Se visualiza un distanciamiento entre emprendedores y la sociedad por la poca participación en ferias que se ve reflejada en bajos niveles de venta de los productos y servicios que ofertan. El uso de redes sociales es limitado a pesar de ser una her-ramienta de difusión de gran impacto. Es importante dar continuidad a los proyectos desde la universidad, puesto que el apoyo debe ser permanente y se posee estudiantes que requieren práctica.
Uniendo científicos con el público general: ejemplos de divulgación científica desde Chile
Authors: Silva Patricia
Published: December 2019

La divulgación científica busca generar un puente entre los científicos y el público general. Divulgar ciencia es importante y necesario por múltiples razones que se mencionan en este ensayo. En este contexto se presentan 5 ejemplos de variadas actividades de divulgación científica realizadas por los autores, de forma tal que puedan servir de inspiración para replicar a conveniencia del investigador y su público. Finalmente se menciona y concluye que realizar divulgación científica es clave, ya que si la sociedad no aprende a valorar la ciencia jamás apoyará debidamente el quehacer científico.
The devil inside that won’t be caged or fixed by words: Fluidity and ethics in Ian McGuire’s The North Water
Authors: Chalupský Petr
Published: December 2019

In Ian McGuire’s novel The North Water (2016), Patrick Sumner, a young medical doctor recently dismissedfrom the British Army with his reputation and professional prospects in ruins, accepts a poorly paid position as a surgeon on a whaling ship in his attempt to flee from his past and his troubled conscience. However, contrary to his expectations, in the Arctic Circle he faces an ordeal far more demanding than anything he has hitherto endured in the form of the harpooner Henry Drax, a dangerous psychopath who is ready to abuse and murder anyone who is an obstacle to the satisfaction of his brutish physical needs. Confronted with violence and cruelty beyond understanding, within the fluid framework of the distorted ethical norms and values of the heterogeneous crew, the embittered Sumner is gradually forced to abandon his protective shell of resigned indifference and reassess the moral stances and responsibilities of a civilized person when faced with human wickedness. Though McGuire acknowledges primarily the inspiration of Herman Melville and Cormac McCarthy, this paper argues that in ethical terms the novel responds to Joseph Conrad’s Lord Jim and Heart of Darkness, pushing the protagonist’s relationship to the other to an extreme by making the other an embodiment of pure evil.
The verbalization of grief in David Grossman’s Falling Out of Time
Authors: Hricková Mária , Klimková Simona
Published: December 2019

David Grossman’s experimental text Falling Out of Time (2011) examines the theme of the death of a child and parents’ attempts to understand and cope with the loss. In order to represent and articulate the sense of unbearable pain and grief, Grossman employs several strategies and techniques related to both content and form which allow for a perspective that is both artistically engaging and sensitive. One of the obvious formal features of the text is his use of poetry, which seemingly represents the most natural means to express the raw emotions and pain of his characters. The paper seeks to examine Grossman’s techniques that help him verbalize the grieving experience of his characters while focusing on his use of poetic language. It seems that the capacity of poetry to rely on meaningful silences and a multilayered interpretive potential enables one to create a healing space which facilitates the process of reconciliation.
Doctor Who meets Loving Vincent Van Gogh (A case study of the “tortured artist” stock character in popular culture)
Authors: Boszorád Martin
Published: December 2019

Methodologically connecting at its core the experience-based and interpretation-based aesthetic approach to popular culture/popular art(s) on one hand and the basis-building views of what is called arch-textual thematology on the other, the paper seeks to examine, following its particular embodiment, one of the most stable, recurring and probably therefore one of the most iconic stock characters - the “tortured artist” stock character. This example of a “stereotyped character easily recognized by readers or audiences from recurrent appearances in literary or folk tradition” (Baldick, 2008, p. 317) can be - besides other principal and distinctive examples such as the “mad scientist”, the “lady/damsel in distress” or, let’s say, the “everyman” - witnessed all across culture, including the sub-sphere of popular culture, and the arts. The implied cultural significance and “omnipresence” of the “tortured artist” stock character can be aptly illustrated by Vincent Van Gogh and not only as a real-life tortured artist prototype or even archetype but also as a popular model for numerous and various cultural depictions - from poems by Charles Bukowski through the “moving pictures” of Loving Vincent to an episode of the well-recognized British TV show Doctor Who.
A voyage from Apollonian Munich to Dionysian Venice in Thomas Mann’s Death in Venice
Authors: Ayyıldız Nilay Erdem
Published: December 2019

This paper explores the intersection of cities and the protagonist’s Apollonian-Dionysian dichotomy in Thomas Mann’s preeminent semi-autobiographical novella Death in Venice (1912) within the cultural and contextual considerations of 20th-century Munich and Venice. The protagonist Gustav von Aschenbach’s oscillation between artistic appreciation and sensual desire is personified by the contrasts Munich draws as a city of enlightenment against Venice which is the city of sensuality and freedom. The article indicates that the narrator associates Nietzsche’s conceptions of the Apollonian and Dionysian parts of human nature with Venice, which acts as a character providing crucial elucidation in regards to the mental state of the protagonist throughout the novella. Thus, the study sheds light upon the symbolic voyage Aschenbach embarks upon - from Apollonian nature to Dionysian nature; from Munich to Venice, where his predominating Dionysian nature burst out through the city, luring him to his own demise.
The typology of backstory inter-character conflicts as a core action in the plot
Authors: Čechová Mariana
Published: December 2019

Using material from classic fairy tales, the author defines three fundamental types of conflict between literary characters in the text model of the fairy-tale world: overt, covert and potential. Their attributes are evidenced and demonstrated via specific texts and their universal (transcultural) analogues are shown in the archnarratives, which go beyond the classic fairy tale genre. At the end of the interpretation, the author proposes a (hypo)thesis that the presented typology could be a starting point for creating a backstory of conflicts as an action-formative factor also in other art genres, and that it can be used as a source for a much broader and modern (and current in contemporary art) diapason of “dramatic” storylines.
Much Ado about Black Naturalism: Don John, Blood, and Caged Birds
Authors: Harris Crystal
Published: December 2019

William Shakespeare’s comedy, Much Ado About Nothing, and Paul Laurence Dunbar’s poem, “Sympathy,” peer through black naturalism’s socially deterministic lens, despite conflicts in time, geopolitics, social norms, and literary imagination. Specifically, Don John’s truculent reference about “sing[ing] in his cage” (1.3.32) inspired investigation into whether Dunbar’s famed line, “I know why the caged bird sings” (21), intentionally alludes to Shakespeare’s work. While the research is inconclusive, the references provide clarity for Don John’s character particularly. Essentially, Don John’s foolhardy evil meets society’s standards for masking social truths, just as Dunbar’s poem has been reduced to a sweet and imaginative ditty over time. Thus, this article broadly explores society’s tendency to recycle oppression under expedient pretenses. Although Don John self-proclaims inherent evil, closer scrutiny of his figurative scar – coat of arms, representing illegitimacy – reveals a socially determined position, more consistent with Dunbar’s second-rate life based on skin color and his naturalism based on whiteness. Because Mowat and Werstine suggest that Don John’s ill-intentioned behaviors are less about biology (blood) than impassioned human response to social injustice (Blood), naturalism links the unlikely pair. As such, the article uses Dunbar’s black naturalism to exemplify societal “caging” in Much Ado and “Sympathy.”
Suspect Survival: Matrophobia in Postmemory Generational Writing
Authors: Kella Elizabeth
Published: December 2019

Family and kinship carry special significance to Holocaust survivors and their descendants. In autobiographies and family memoirs, writers of what Marianne Hirsch terms the postmemory generation employ different narrative strategies for coming to terms with the ways in which the Holocaust has marked their identities and family ties. This article focuses on women’s writing of the postmemory generation, examining three works in English by daughters of survivors in the UK, the US, and Canada, written during the 1990s. It investigates the narrative strategies used by Anne Karpf, Helen Fremont, and Lisa Appignanesi to represent maternal sexual agency and vulnerability in a survival context. It suggests that these representations are strongly influenced by matrophobia and matrophilia, defined as the conflicting dread of becoming and desire to be one’s mother, which are themselves strongly conditioned by Holocaust history, particularly the gendered history of vulnerability among women in open hiding during the war1.
Political Theatre in the Age of Brexit: The State of Nation in Monologues
Authors: Öğünç Banu
Published: December 2019

On the British stage, political theatre, which emerged in the twentieth century, has been linked with the problems of the working class as initiated in the 1920s until the early 1960s. With the end of the official censorship of theatre in 1968, political theatre in Britain experienced a period in which socialist works marked the stage. Nevertheless, the 1990s challenged the association of political theatre with the conditions of the working class. Considering the current political and social events in Britain and around the world, it is appropriate to underline that political theatre is not only in a constant flux, but its definition has been once again challenged. In this regard, Brexit can be considered as one of the most significant movements to influence the understanding of political theatre in the twenty-first century. Consequently, this study aims to analyse Brexit Shorts: Dramas from a Divided Nation (2017), a co-production by the Guardian and Headlong Theatre Company and discuss their contribution to the changing definition of political theatre. Brexit Shorts will be further explored regarding their influence on the popularity of monologues as a mode of performance.
How the Booker Prize Won the Prize
Authors: Moseley Merritt
Published: December 2019

This article shows that the Booker Prize for fiction, which is neither the oldest nor the richest award given for novels in English, is nevertheless widely conceded to be the pre-eminent recognition. Sometimes it is called the “most significant”; sometimes the “most famous”; ultimately these two qualities are inseparable. I canvass some of the explanations for the Booker’s position as top prize and argue that the most important reasons are Publicity, Flexibility, and Product Placement. The Booker has managed its public image skillfully; among the devices that assure its continued celebrity is the acceptance, almost the courting, of scandal. Flexibility is partly a function of the practice of naming five new judges each year, but the Booker has also been responsive to challenges, including the recognition that it paid too little attention to female authors. The decision to admit American books into the competition was a sign of flexibility, as it was a guarantee of scandal. And the Booker has followed a path of “product placement” that positions it accurately between demands for high art and for “readability,” as examination of several periods in its history demonstrate.
Coenzyme and cofactor metabolism belongs to biochemical processes significantly regulated in human granulosa cells collected after IVF during long-term primary in vitro culture
Authors: Nawrocki Mariusz J. , Sibiak Rafał , Brązert Maciej , Celichowski Piotr , Pawelczyk Leszek , Chermuła Błażej , Dompe Claudia , Kempisty Bartosz , Mozdziak Paul
Published: December 2019

Granulosa cells (GCs) provide the microenvironment necessary for the development of the follicle and the maturation of the oocyte. GCs are associated with reproductive system function and the maintenance of pregnancy by participating in the synthesis of steroid hormones. Many authors point to new ways of using GCs in regenerative medicine and indicate the significant plasticity of this cell population, suggesting that GCs can undergo a transdifferentiation process. Employing primary in vitro cell cultures and high-throughput transcriptome analysis via Affymetrix microarrays, this study describes groups of genes associated with enzymatic reactions. 52 genes were identified belonging to four gene ontology biological process terms (GO BP): “coenzyme biosynthetic process”, “coenzyme metabolic process”, “cofactor biosynthetic process” and “cofactor metabolic process”. All identified genes showed reduction in the level of mRNA expression during long-term in vitro cultivation. Significanthe transcriptomic profile variability was exhibited for the genes (ELOVL5, ELOVL6 and GPAM) involved in enzymatic regulation of fatty acid metabolism.

Running title: Enzymatic regulation in granulosa cells
Nucleotide, ribonucleotide and ribonucleoside binding belongs to differentially expressed genes in porcine epithelial oviductal cells during longterm primary cultivation
Authors: Nawrocki Mariusz J. , Sibiak Rafał , Kałużna Sandra , Brązert Maciej , Celichowski Piotr , Pawelczyk Leszek , Moncrieff Lisa , Kempisty Bartosz , Mozdziak Paul
Published: December 2019

The oviduct play a crucial role in reproductive process, through facilitating successful embryo growth and conception. Oviduct activity is orchestrated by various factors, depending on cyclic dynamics, which crucially affect the success of reproductive function. The morphological modifications of oviducts in response to the female reproductive cycle are well established. However, detailed characterization at the molecular level is still needed. The present study, employed primary in vitro cell cultures and high-throughput transcriptome analysis via an Affymetrix microarray approach, described nucleotide, ribonucleotide and ribonucleoside binding patterns at a molecular level in oviduct epithelial cells (OECs). 222 genes were targeted belonging to four gene ontology biological process terms (GO BP): “adenyl nucleotide binding”, “adenyl ribonucleotide binding”, “ribonucleotide binding”, “ribonucleoside binding”, which showed the greatest variability in the level of mRNA expression during of long-term cultivation. In this group of genes, special attention was paid to those showing the greatest variability in relation to the reference measurement, including OASL, PIM1, ACTA2 and ABCA1.

Running title: Oviductal nucleotide and nucleoside binding patterns
Overview of methods of isolation, cultivation and genetic profiling on human umbilical cord stem cells
Authors: Stefańska Katarzyna , Sibiak Rafał , Dompe Claudia , Moncrieff Lisa , Hutchings Greg , Janowicz Krzysztof , Kempisty Bartosz
Published: December 2019

Stem cells possess unique properties, such as self-renewal ability or differentiation capacity into more specialized cells, which makes them particularly relevant for regenerative medicine and cellular therapies. Although embryonic stem cells (ESCs) are capable of differentiation into all cell lineages, their utilization is associated with ethical concerns since they are obtained from embryos. Furthermore, ESCs may form teratomas or cause immune rejection in the clinical setting. Therefore, an effort has been made to utilize stem cells derived from adult tissues, especially mesenchymal stem cells (MSCs). A particularly attractive source of MSCs is the human umbilical cord, which is typically discarded after birth and considered a medical waste, therefore the acquisition of the cells is not associated with any health risk for a patient. Moreover, umbilical cord-derived MSCs do not express MHCII, thus they exhibit reduced immunogenicity. MSCs have been isolated from all compartments of umbilical cord, however the Wharton’s jelly-derived MSCs (WJ-M-SCs) are the most clinically utilizable. There are two techniques of UC-MSCs isolation: the enzymatic and explant procedures. The explant method involves cell outgrowth of tissue pieces placed into plastic culture vessel after mechanical splitting, whereas the enzymatic technique involves minced tissue digestion in an enzymatic solution. In vitro culture conditions of the isolated cells are highly variable among the researchers, however the most commonly performed molecular assays are homogenous and include: RT-qPCR, flow cytometry and immunocytochemistry.

Running title: Human umbilical cord stem cells isolation, cultivation and genetic profiling
Ultrastructural variability of macrophages in the wall of selected aorto-coronary bypass grafts
Authors: Perek Bartłomiej , Kowalska Katarzyna , Malińska Agnieszka , Jemielity Marek , Nowicki Michał
Published: December 2019

Macrophages, detected as CD68+ cells, are considered to have marked contribution to aorto-coronary grafts disease. The purpose of this study was to find any ultrastructural differences in CD68+ cells between arterial and venous aorto-coronary grafts.

The surplus segments of radial artery (RA) and saphenous vein (SV) were obtained from 50 patients with the mean age of 63.4±9.2 years who undergo elective coronary artery bypass grafting (CABG). The vascular segments were analyzed by means of both light (to assess number and distribution of macrophages within their walls) and transmission electron microscopy (to evaluate ultrastructure of CD68+ cells in the vessel layers).

Histological analysis revealed that not only more macrophages (median (25th; 75th percentile)) were found on the transverse sections of veins (95 (67; 135)) than arteries (66 (43; 108)) (p<0.05) but also at least of 50% of them were found in the tunica intima and tunica media in SV while only 30% in RA. TEM studies showed that biological activity of macrophages depended on CD68+ location and was irrespective of the vessel type. Those found in the tunica intima and tunica media presented ultrastructure typical for active cells rich in numerous lysosomes, well developed rough endoplasmic reticulum and Golgi apparatus whereas adventitial macrophages for unreactive residual cells.

Ultrastructural characteristics of both forms of macrophages infiltrating wall of aorto-coronary grafts is similar irrespective of the vessel type. More active cells in the inner layers of the venous conduits may contribute to their inferior outcomes compared to the arteries.

Running title: Macrophages and aorto-coronary grafts
Morphological Characteristics of Skin Lesions in Cattle Naturally Infected with Lumpy Skin Disease Virus in Serbia
Authors: Vasković Nikola , Debeljak Zoran , Vidanović Dejan , Šekler Milanko , Matović Kazimir , Aničić Milan , Marinković Darko
Published: December 2019

Lumpy skin disease (LSD) is a disease of cattle and domestic buffaloes caused by Capripoxvirus which can lead to significant economic losses. Until several years ago it was limited to Africa and the Middle East, but recently it was reported in Turkey (2013), Greece (2015), and in 2016 it spread to Bulgaria, North Macedonia, Albania, Montenegro and Serbia. The aim of this study was to determine the microscopic lesions in the skin of naturally infected animals, highlight their features and compare them to the findings of other authors. Gross lesions in the skin of cattle infected with Lumpy skin disease virus (LSDV) were manifested in the form of skin nodules which were round, raised, clearly circumscribed, firm and randomly distributed in the whole skin including the vulva and udder. Histopathological lesions included degeneration of epithelial cells, hyperkeratosis, spongiosis, and acanthosis present in 85.88%, 81.18%, 84.71%, and 80.0% of samples, respectively. Intracytoplasmic inclusion bodies were noted in keratinocytes of the stratum spinosum in 56.47% of samples, and in hair follicle cells and sebaceous glands epithelial cells in 45.88% of samples. Cutaneous lesions were in the form of inflammatory infiltrate present in the dermis and subcutis in 97.65% of samples. Since there are only a few literature data in this field, this study expands the knowledge relating to morphological alterations in LSD. Based on the characteristic microscopic lesions described in the present study, histopathology should be considered as a very useful method for the diagnosis of LSD.
Differentiation of Anatomic Entities in the Dog Stifle Joint Following S10B Plastination: Comparative Colorimetric and Radiological Investigations
Authors: Bakici Caner , Akgun Remzi Orkun , Ekim Okan , Insal Burcu , Kaya Ufuk , Bilgili Hasan , Bumin Ali , Cakir Ahmet
Published: December 2019

The knee joint is not only the most important and complex structure to present during anatomy lectures, but also a significant region for radiologists and orthopedicians. The objectives of this paper are: 1) to evaluate the efficacy of plastinated specimens by measuring colorimetric differentiation and 2) to compare the anatomical structures of the images obtained from computed tomography (CT) and silicone plastination cross-sections and perform three-dimensional (3D) reconstructed models of the specimens. A total of 16 knee joints selected from the 10% formalin fixed/dissected group (n=6), non-fixed/dissected group (n=6) and non-dissected whole knee joint group (n=4) were scanned by CT and plastinated by using the S10B silicone technique. The color changes of the structures were quantitatively determined with the colorimeter device. Plastinated whole knee joints were cut transversally with a band saw and compared with CT images. Finally, 3D reconstruction of the knee joints was performed from CT images with the 3D reconstruction program. There were statistically significant differences between the fixed and non-fixed groups in terms of color changes (p<0.001). The anatomical structures were identified and matched in the cross-sections of plastinates and corresponding CT images. It was observed that plastinated samples were elastic, durable and non-hazardous specimens to use in the veterinary orthopedics and anatomy courses and lectures. It has been found that fixation was useful for the final product morphology. It is thought that the 3D images obtained from this study will support comprehension of the relationships between the bones, muscles, and ligaments.
Duplex PCR for Detection of Aleutian Disease Virus from Biological and Environmental Samples
Authors: Kowalczyk Marek , Jakubczak Andrzej , Gryzińska Magdalena
Published: December 2019

Aleutian disease is one of the most serious disease entities affecting mink farms. The disease causes significant economic losses in mink breeding countries. The aim of the study was to optimize a diagnostic test based on duplex PCR to enable detection of Aleutian disease virus in biological and environmental samples.

Blood (n = 40) and spleen (n = 40) samples from animals with suspected infection, and swabs from cages in which infected animals were kept (n = 20) were used for analysis. DNA was isolated from the samples, followed by optimization of the duplex PCR reaction targeting sequences coding NS1 and VP2 proteins. The qPCR method was used to determine the sensitivity of the reaction. The specificity of the analysis was confirmed by the sequencing results.

Optimized duplex PCR enabled detection of Aleutian Mink Disease Virus (AMDV) genetic material in biological and environmental samples. Testing of the sensitivity of the method indicated clear amplification for both primer pairs at 102 copies of viral DNA in a reaction. Sequencing confirmed the specificity of the reaction, which in the case of both primer pairs indicated an over 90% agreement between the isolates and the variants of the virus from the databases.

The use of duplex PCR to detect two regions of the AMDV genome may increase the sensitivity and specificity of the method and significantly expand the possibilities of further analysis based on sequencing.
Ascaridia Galli Infection in Laying Hens and the Results of in Vitro Efficacy of Levamisole, Piperazine and Carvacrol, Whether is Necessary to Change the Deworming Protocols?
Authors: Rajković Milan , Vučićević Ivana , Vučićević Miloš , Došenović Milan , Charvet L. Claude , Resanović Radmila , Trailović M. Saša
Published: December 2019

We present a case of Ascaridia galli infection in laying hens on a farm in central Serbia. During the rearing period on litter, ascaridosis was diagnosed at 15 weeks of age by routine parasitological fecal examination. Pullets were treated with flubendazole for one week, and two weeks later the hens were moved to battery cages. The production results were within technological standards until the 23rd week and the medical health status was without any clinical symptoms. After that period weight loss began, the egg production dropped to 70% and eggs were of poor quality. Subsequently, severe feather pecking and an increase of mortality were reported. The postmortal examination showed severe anemia and several gross lesions in the liver, intestines, lungs, and kidneys. Different sizes of A. galli were found in the lumen of the duodenum and jejunum. Gross changes of the intestinal mucosa were present, such as a thickened intestinal wall with hemorrhagic spots, inflammation and necrotic patches. Histopathological examination showed marked changes in the intestines, liver and kidneys. All visible live parasites were collected and stored in Earle’s balanced salts, and females were used for in vitro susceptibility testing. Median lethal concentration (LC50) of piperazine, levamisole and carvacrol for A. galli was 119.7μM, 2.71μM and 3.26μM, were applied, respectively. Based on our results, it is likely that reinfection occurred after completed dehelmintization. In relation to the new circumstances and the regulation for laying hen welfare the deworming protocol should be changed in order to ensure successful dehelmintization. In order to prevent reinfection the treatment must be done at the end of the rearing period and thus be maximally effective.
Chromosome Aberrations Produced by Mestranol in Human Lymphocyte Cultures
Authors: Teodorović Radislava , Drašković Vladimir , Đurić Spomenka , Nenadović Kartarina , Mirilović Milorad , Janković Ljiljana
Published: December 2019

In this investigation, the genotoxic properties of mestranol were examined in vitro. Human lymphocyte cultures were exposed for 72 h to mestranol at concentrations of 7.5, 15 and 30 µg/g. The genotoxic effects of the chemosterilant were assessed by numerical and structural chromosome aberrations.

Mestranol induced certain genotoxic effects in human lymphocytes. There was a dose-dependent significant (p<0.01) increase in the number of numerical aberrations in comparison to the control, but without significant differences (p>0.05) between the doses applied. Further, structural aberrations increased significantly (p<0.01) in the presence of mestranol, being most frequent in cultures exposed to the highest mestranol dose.

The frequency of Robertsonian translocations increased significantly only in cultures treated with mestranol at concentration of 30 µg/g in comparison both with the control (p<0.01) and the lowest chemosterilant dose (p<0.01).

There were significant differences (p<0.01) in the levels of chromosome gaps and fragments compared to Robertsonian translocations, whilst the frequencies between gaps and fragments were not significantly different (p>0.05).
Effect of Season on Hematologic, Biochemical, and Hormonal Analytes in Rams of Two Breeds
Authors: Oikonomidis Ioannis L. , Kiossis Evangelos , Brozos Christos , Kritsepi-Konstantinou Maria
Published: December 2019

Clinicopathological investigations are essential for the evaluation of the health status of ruminants. Apart from species-specific reference intervals, the effect of common biological factors should be considered for an accurate interpretation of laboratory data. The aim of this study was to evaluate the effect of season on hematologic and biochemical analytes, and serum total thyroxine and cortisol in adult rams of two breeds. Four blood samples (one every season) were collected from each ram. Complete blood count was performed on the Advia 120 (Siemens Healthcare Diagnostics, USA), while the differential leukocyte count was manually conducted. Biochemical and hormonal analyses were performed on Flexor E (Vital Scientific, The Netherlands), AVL 9180 (Roche Diagnostics, Belgium), and Immulite 1000 (Siemens Healthcare Diagnostics, USA), respectively. Linear mixed effects models (R language) were employed for statistical analyses. Forty-three (26 Chios, 17 Florina), adult, clinically healthy rams were included. Statistically significant (p<0.05), mostly breed-independent seasonal differences were observed in almost all of the analytes. However, when assessing these differences in view of the respective reference intervals, only a few of them were considered biologically important. Specifically, mild hyperglycemia and mild decrease in the concentration of total calcium and inorganic phosphorus were detected in winter, while a mild increase in thyroxine concentration (autumn) and creatine kinase activity (spring and summer) was also noted. In conclusion, seasonal effects should be considered when evaluating laboratory results in rams; however, season does not appear to have an essential effect on the clinicopathological profile of rams reared in the Mediterranean region.
Penile Foreign Bodies in Dogs: A Retrospective Study
Authors: Bergamini Ilaria , Linta Nikolina , Gaspardo Alba , Cunto Marco , Peli Angelo , Zambelli Daniele , Pietra Marco
Published: December 2019

Hematuria, or preputial hemorrhagic discharge, is an extremely common clinical sign; it can be associated with a wide range of diseases, including, even if only rarely, penile foreign bodies.

The aim of this retrospective study was to describe the diagnosis and therapy involving migration from the preputial ostium or penile urethra of a foreign body (awn grass) embedded in the connective tissue surrounding the penis, or in deeper inguinal tissues, in dogs with hematuria or preputial hemorrhagic discharge.

In the selected cases, signaling, history, clinical signs, results of laboratory exams, endoscopic and ultrasonographic evaluation, and the technique used for foreign body removal were evaluated.

Dogs with hemorrhagic discharge consequent to a penile foreign body represented 2% of the entire population considered. At physical examination, the most common features were the presence of swelling of the glans and hyperemia associated with a penile fistula (4/6 dogs), and pain during penile exteriorization (3/6 dogs). Laboratory results showed mild neutrophilic leukocytosis in 2/3 dogs and reticulocytosis in 1/3 dogs. Endoscopy, performed in 2/6 dogs, did not reveal any alterations associated with passage of the foreign body. Ultrasonography was useful in reaching a definitive diagnosis, identifying the position of the grass awn in 6/6 cases and permitting its removal in all dogs using an ultrasound-guided technique.

This case report suggested that penile foreign bodies are a rare, but possible, cause of hematuria or hemorrhagic discharge in male dogs, and that ultrasonography is a useful technique in the making of a differential diagnosis and removal of foreign bodies.
Pericardial Patch as a Palliative Treatment in Three Dogs Affected by Unresectable Cardiac Tumor
Authors: Collivignarelli Francesco , Vignoli Massimo , Del Signore Francesca , Simeoni Francesco , Tamburro Roberto
Published: December 2019

This report is aimed at describing a pericardial patch for unresectable cardiac tumors as a palliative technique to prevent pleural effusion.

Each dog underwent the same diagnostic protocol including a cardiological assessment, hematology and biochemistry profile, urinary test, abdominal and heart ultrasonography and chest radiographs. Computed tomography was always performed in order to exclude any potential metastatic lesions. All dogs underwent open pericardiectomy: the pericardium was opened and transposed on the mass according to its shape. Biopsy of the mass was always performed. Histopathological evaluation of the masses described two HAS and one chemodectoma. Chemotherapy protocol includes doxorubicin that was weekly administered. Dogs with HSA survived 65 and 85 days respectively without pleural effusion, while the dog with chemodectoma died after six months for reasons unrelated to the tumor.

No evidence of pleural effusion was observed during the heart recheck, monthly performed.

Pericardial patch is a potential choice as a palliative treatment for unresectable cardiac tumors. No pleural effusion was seen in three dogs affected by unresectable cardiac tumors.
Acute administration of nicotine does not enhance cognitive functions
Authors: Grus Andrea , Hromatko Ivana
Published: December 2019

Chronic smokers often claim that smoking improves their cognitive abilities, such as concentration. However, scientific evidence to support this claim is scarce. Previous studies gave inconclusive results, and some of them had significant methodological flaws. Therefore, the aim of this study was to test whether smoking a single cigarette affects performance across several cognitive domains. It included a group of 22 occasional smokers aged 19–29 years. Attention, working memory, and visuospatial reasoning were assessed using a within-subjects design with a control setting. There were two separate testing sessions two days apart. Half the group started with experimental and the other half with control setting. In the experimental setting, the participants completed the first block of tasks, smoked one cigarette (with a nicotine yield of 0.5 mg), and then completed the second block of tasks. In the control setting, the procedure was the same, except that the participants had a glass of water instead of a cigarette. Repeated measures ANOVA showed no significant effects of cigarette smoking on either reaction time rates or accuracy on any of the three cognitive domains. These results suggest that, at least among young, occasional smokers, smoking does not affect cognition and the claims of its improvement are probably a result of some sort of cognitive bias.