bluuebunny/crossref_metadata_2025 huggingface dataset:
Precise and versatile microplate reader-based analyses of biosensor signals from arrayed microbial colonies
Authors: Hartmann Fabian S. F. , Weiß Tamara , Kastberg Louise L. B. , Workman Christopher T. , Seibold Gerd M.
Published: August 2022

Genetically encoded fluorescent biosensors have emerged as a powerful tool to support phenotypic screenings of microbes. Optical analyses of fluorescent sensor signals from colonies grown on solid media can be challenging as imaging devices need to be equipped with appropriate filters matching the properties of fluorescent biosensors. Towards versatile fluorescence analyses of different types of biosensor signals derived from arrayed colonies, we investigate here the use of monochromator equipped microplate readers as an alternative to imaging approaches. Indeed, for analyses of the LacI-controlled expression of the reporter mCherry inCorynebacterium glutamicum, or promoter activity using GFP as reporter inSaccharomyces cerevisiae, an improved sensitivity and dynamic range was observed for a microplate reader-based analyses compared to their analysesviaimaging. The microplate reader allowed us to capture signals of ratiometric fluorescent reporter proteins (FRPs) with a high sensitivity and thereby to further improve the analysis of internal pH via the pH-sensitive FRP mCherryEA inEscherichia colicolonies. Applicability of this novel technique was further demonstrated by assessing redox states inC. glutamicumcolonies using the FRP Mrx1-roGFP2. By the use of a microplate reader, oxidative redox shifts were measured in a mutant strain lacking the non-enzymatic antioxidant mycothiol (MSH), indicating its major role for maintaining a reduced redox state also in colonies on agar plates. Taken together, analyses of biosensor signals from microbial colonies using a microplate reader allows comprehensive phenotypic screenings and thus facilitates further development of new strains for metabolic engineering and systems biology.
Inhibition of Talin-induced Integrin Activation by a Double-hit Stapled Peptide
Authors: Gao Tong , Cho Eun-ah , Zhang Pingfeng , Wu Jinhua
Published: August 2022

Integrins are ubiquitously expressed cell-adhesion proteins. Talin is required for integrin activation through an inside-out signaling pathway, during which talin is recruited to the plasma membrane (PM) by RAP1 directly or through its effector, RAP1-Interacting Adaptor Molecule (RIAM). RIAM also activates talin from autoinhibition by binding to talin head domain. A helical talin-binding segment (TBS) in RIAM mediates both talin activation and recruitment by binding to two distinct sites in talin head and rod domains respectively. The bi-specificity of the TBS fragment allows us to develop a new strategy to suppress talin-induced integrin activation through a “double-hit” approach. We designed an experimental peptidomimetic inhibitor by engineering a hydrocarbon “staple” in the helical TBS fragment to mask the integrin binding site in the talin head. The stapled peptide (S-TBS) exhibits a stronger binding affinity with talin and inhibits talin:integrin interaction. Crystal structure of S-TBS in complex with talin rod domains reveals an interface that overlaps with the TBS-binding site in talin. Consistently, S-TBS also exhibits an inhibitory effect on TBS:talin-rod interaction during the PM recruitment of talin. Importantly, S-TBS possesses excellent cell permeability and inhibits integrin activation in a talin-dependent manner. Hence, our results present a novel approach to design a new class of intracellular inhibitors targeting integrin functions.
Comparative analysis of actin visualization by genetically encoded probes in cultured neurons
Authors: Ignácz Attila , Nagy-Herczeg Domonkos , Hausser Angelika , Schlett Katalin
Published: August 2022

Actin cytoskeleton predominantly regulates the formation and maintenance of synapses by controlling dendritic spine morphology and motility. To visualize actin dynamics, actin molecules can be labelled by genetically fusing fluorescent proteins to actin monomers or using fluorescently tagged actin-binding proteins or single-chain anti-actin antibodies. However, the effects of these labels on the morphology of neurons have not been quantitatively compared yet. In the present study, we analysed Actin-Chromobody-GFP, LifeAct-GFP and EGFP-actin with respect to their effects on actin-related features in mouse cultured hippocampal neurons.

The actin-binding probes LifeAct and Actin-Chromobody showed similar affinity to F-actin, and along with EGFP-actin, were enriched in dendritic protrusions. In contrast to EGFP-actin, neither of these constructs was able to detect subtle changes in actin remodelling between mature mushroom shaped spine and less developed filopodia. None of the compared probes altered filopodial motility compared to control EGFP expression, however, within 24 hours expression, minor changes in dendritic spine morphology and density were visible. Furthermore, while EGFP-actin and LifeAct-GFP expression did not alter dendritic arborization, AC-GFP expressing neurons displayed a reduced dendritic arborization. We therefore conclude that careful consideration of cellular consequences is required before performing experiments with a particular actin labelling probe in primary neurons.
Detecting isoform-level allelic imbalance accounting for inferential uncertainty
Authors: Wu Euphy , Singh Noor P. , Choi Kwangbom , Zakeri Mohsen , Vincent Matthew , Churchill Gary A. , Ackert-Bicknell Cheryl L. , Patro Rob , Love Michael I.
Published: August 2022

Allelic imbalance (AI) of gene expression in heterozygous individuals is a hallmark of cis-genetic regulation, revealing mechanisms underlying the association of non-coding genetic variation with downstream traits, as in GWAS. Most methods for detecting AI from RNA-sequencing (RNA-seq) data examine allelic expression per exonic SNP, which may obscure imbalance in expression of individual isoforms. Detecting AI at the isoform level requires accounting for inferential uncertainty (IU) of expression estimates, caused by multi-mapping of RNA-seq reads to isoforms and alleles. Swish, a method developed previously for differential transcript expression accounting for IU, can be applied in a paired setting to detect AI. However, in AI analysis, most transcripts will have high IU across alleles such that even methods like Swish will lose power. Our proposed method, SEESAW, offers AI analysis at various level of resolution, including gene level, isoform level, and optionally aggregating isoforms within a gene based on their transcription start site (TSS). This TSS-based aggregation strategy strengthens the signal for transcripts that may have high IU with respect to allelic quantification. SEESAW is primarily designed for experiments with multiple replicates or conditions of organisms with the same genotype, as in an F1 cross or time course experiments of cell lines. Additionally, we introduce a new test for detecting AI that changes across a continuous covariate, as in a time course experiment. The SEESAW suite of methods is evaluated both on simulated data and applied to an RNA-seq dataset of differentiating F1 mouse osteoblasts.
Trypanosoma brucei Mitochondrial DNA Polymerase POLIB Contains a Novel Thumb Insertion That Confers Dominant Exonuclease Activity
Authors: Delzell Stephanie B. , Nelson Scott W. , Frost Matthew P. , Klingbeil Michele M.
Published: August 2022

Trypanosoma brucei and related parasites contain an unusual catenated mitochondrial genome known as kinetoplast DNA (kDNA) composed of maxicircles and minicircles. The kDNA structure and replication mechanism are divergent and essential for parasite survival. POLIB is one of three Family A DNA polymerases independently essential to maintain the kDNA network. However, the division of labor among the paralogs, particularly which might fulfill the role of a replicative, proofreading enzyme remains enigmatic. De novo modeling of POLIB revealed a structure divergent from all other Family A polymerases in which the thumb subdomain contains a 369 amino acid insertion with homology to DEDDh DnaQ family 3’-5’ exonucleases. Here we demonstrate recombinant POLIB 3’-5’ exonuclease prefers DNA vs. RNA substrates and degrades single- and double-stranded DNA in a non-processive manner. The exonuclease activity prevails over polymerase activity on DNA substrates at pH 8.0, while DNA primer extension is favored at pH 6.0. Mutations that ablate POLIB polymerase activity slow the exonuclease rate suggesting crosstalk between the domains. We show that POLIB is able to extend an RNA primer more efficiently than a DNA primer in the presence of dNTPs but does not incorporate rNTPs efficiently using either primer. Immunoprecipitation of Pol I-like paralogs from T. brucei corroborate the pH selectivity and RNA primer preferences of POLIB and revealed that the other paralogs efficiently extend a DNA primer. The unique POLIB thumb insertion influences the balance between polymerase and exonuclease activity and provides another example of exquisite diversity among DNA polymerases for specialized function.
Mechanical compression induces persistent bacterial growth during bacteriophage predation
Authors: Mason Guy , Rojas Enrique R.
Published: August 2022

Although the relationship between bacteria and lytic bacteriophage is fundamentally antagonistic, these microbes not only coexist but thrive side-by-side in myriad ecological environments. The mechanisms by which coexistence is achieved, however, are not fully understood. By examining Escherichia coli and bacteriophage T7 population dynamics at the single-cell and single-virion level using a novel microfluidics-based assay, we observed bacteria growing “persistently” when perfused with high-titer bacteriophage. Persistence occurred at a frequency five orders of magnitude higher than is expected from natural selection of bacteriophage-resistant mutants. Rather, the frequency of persistence was correlated with the degree to which the bacteria were mechanically compressed by the microfluidic perfusion chamber. Using a combination of mutagenesis and fluorescent imaging techniques, we found that compression induces persistence by activating the Rcs phosphorelay pathway, which results in the synthesis of extracellular capsule that sterically blocks bacteriophage adsorption. Other forms of mechanical stimulation also promoted Rcs activity and persistence. These findings have important implications for our understanding of microbial ecology in many important environments, including the gut and the soil, where bacteria grow in confinement.

Significance Statement

Bacteria and bacteriophage form one of the most fundamental and important predator-prey relationships on earth, yet the factors that promote long-term stability of their populations are unknown. Here, we demonstrate that Escherichia coli is able to rapidly grow during bacteriophage predation if they are doing so in spatially confined environments. This discovery revises our understanding of bacteria-bacteriophage population dynamics in many real-world environments where bacteria grow in such environments, such as the gut and the soil. Additionally, this result has critical implications for the potential of antibacterial therapies to function during pathogenesis, when bacteria are also mechanically stimulated.
Plot-based approach for controlled morphological transformation in urban heritage contexts: the case of New Gourna village, Luxor – Egypt
Authors: AlSadaty Aliaa
Published: August 2022

Purpose

As historic cities evolve, change in their urban form can be expected. Yet, uncontrolled change of land plots, which represent a significant element of urban form, leads to uncontrolled change in buildings' configurations and typologies threatening accordingly the urban character of heritage contexts. Mechanisms controlling plot subdivision, however, can play an effective role in guiding developments and in controlling urban change in heritage settings. The present study seeks to assist decision-makers in their attempt to control urban change in heritage areas through a plot-based approach.

Design/methodology/approach

This paper is divided into three sections: the first focuses on plot subdivision as a key aspect of urban form; the second illustrates the case of the heritage village of New Gourna in Luxor and the third includes discussion and implications of adopting a plot-based morphological approach to control morphological transformation in heritage contexts. For the morphological analysis of New Gourna, the study relied on comparative cartographic analysis of original drawings of the village versus the situation in 2022. The morphological analysis focuses mainly on qualitative and quantitative aspects of plot configurations and building patterns.

Findings

Findings support the urgency of establishing a plot-based strategy to maintain urban character of heritage contexts in Egypt and call for a plot-based morphological approach to control change and inform new development attempts.

Originality/value

The present research provides an assessment of the morphological transformation of the heritage village of New Gourna. In addition, it proposes a plot-based approach for heritage contexts under transformation.
System reliability analysis of foundation stability of gravity dams considering anisotropic seepage and multiple sliding surfaces
Authors: He Jia-Nan , Yang De-wei , Zhenyu Wu
Published: August 2022

Purpose

For gravity dams built on foundations with directional joint sets, the seepage in the foundation possesses anisotropic characteristics and may have adverse effects on the foundation stability. A methodology for system reliability analysis of gravity dam foundations considering anisotropic seepage and multiple sliding surfaces is proposed in this paper.

Design/methodology/approach

Anisotropic seepages in dam foundations are simulated using finite element method (FEM) with the equivalent continuum model (ECM), and their effect on dam foundation stability is involved by uplift pressures acting on the potential sliding surfaces. The system failure probability of the dam foundation is efficiently estimated using Monte Carlo method (MCM) combined with response surface method (RSM).

Findings

The case study shows that it is necessary to consider the possibly adverse effect of anisotropic seepage on foundation stability of gravity dams and the deterministic analysis of the foundation stability may be misleading. The system reliability analysis of the dam foundation is justified, as the uncertainties in shear strength parameters of the foundation rocks and joint sets as well as aperture, connectivity and spacing of the joint sets are quantified and the system effect of the multiple potential sliding surfaces on the foundation reliability is reasonably considered.

Originality/value

(1) A methodology is proposed for efficient system reliability analysis of foundation stability of gravity dams considering anisotropic seepage and multiple sliding surfaces (2) The influence of anisotropic seepage on the stability of gravity dam foundation  is revealed (3) The influence of estimation errors of RSMs on the system reliability assessment of dam foundation is investigated.
Salaam Bombay Foundation: challenges in integrating skills, education and job markets for adolescents in India
Authors: Galliara Meena , Sisodia Swati , Nagpal Pragya
Published: August 2022

Learning outcomes

The learning outcomes are as follows: to analyze the driving forces that lead non-government organizations (NGOs) to develop need-based programs; to evaluate the strategy adopted by NGOs in attaining the organization’s mission and creating a social impact, corporate social responsibility, inclusion, labor market, social enterprise, strategy and vocational learning; to apply social business canvas for analyzing the business model deployed by NGOs to develop market linkages; to analyze the challenges in setting and scaling NGO programs and strategies designed to address the same; and to enable students to brainstorm in creating future growth options for scaling up and replicating NGO programs.

Case overview/synopsis

The case describes the journey of Salaam Bombay Foundation (SBF), a national-level NGO registered in 2002 in Mumbai, India. In March 2020, SBF had an annual budget of INR 13.98 crores (US$1.84m). It addresses the challenging environments children from economically constrained families face by engaging them in continuing school education and providing vocational training. Since its inception, SBF has launched and executed many in-school and after-school programs. To successfully transit skilled adolescents and teenagers into the labor market and help them make informed career decisions, SBF launched “DreamLab,” a stipend-based “internship” model, in August 2018. Gaurav Arora, Vice President SBF, was assigned the responsibility to scale up skills@school and DreamLab internship programs. With disruptions caused by the pandemic in March 2020, Arora struggled to operationalize DreamLab as initially planned. The case is at a crucial decision point where clouds of uncertainty have made Arora and his team anxious about their future course of action.

Complexity academic level

The case is intended for students of undergraduate and graduate programs in Business Management, Social Entrepreneurship and Social Work programs. Executives of management development programs can also use the case to analyze the effectiveness and management of the skill development program.

Supplementary materials

Teaching notes are available for educators only.

Subject code

CSS 3: Entrepreneurship.
The future of e-commerce? Understanding livestreaming commerce continuance usage
Authors: Chong Han Xi , Hashim Ahmad Hariza , Osman Syuhaily , Lau Jasmine Leby , Aw Eugene Cheng-Xi
Published: August 2022

Purpose

The purpose of this paper is to identify the continuance usage of livestreaming commerce. The proposed antecedents of livestreaming commerce continuance usage are social presence, perceived crowdedness, need for arousal, susceptibility to informational influence, and perceived value, whilst trust in broadcasters is treated as the moderating factor.

Design/methodology/approach

A survey was conducted using a questionnaire designed specifically for this purpose, which was completed by 388 Malaysian consumers aged 26–40 years who have prior experience with livestreaming commerce. The authors tested the framework using the partial least squares structural equation modelling (PLS-SEM) technique and complementary by performing artificial neural network (ANN) analysis.

Findings

The results indicate several significant relationships, whereby social presence, perceived crowdedness, susceptibility to informational influence, and trust in broadcasters significantly influence perceived value, whereas perceived value significantly influences continuance intention. In addition, trust in broadcasters moderates the relationship between perceived value and continuance intention.

Originality/value

The current study adds significantly to the body of knowledge about livestreaming by examining the direct determinants of perceived value and thus delineating how perceived value influences continuance intention. The findings yielded also illustrate the role of trust in broadcaster as the moderator of the link between perceived value and continuance intention.
Enhanced DWA algorithm for local path planning of mobile robot
Authors: Lai Xin , Wu Dan , Wu Di , Li Jia He , Yu Hang
Published: August 2022

Purpose

The purpose of this study is to solve the problems of poor stability and high energy consumption of the dynamic window algorithm (DWA) for the mobile robots, a novel enhanced dynamic window algorithm is proposed in this paper.

Design/methodology/approach

The novel algorithm takes the distance function as the weight of the target-oriented coefficient, and a new evaluation function is presented to optimize the azimuth angle.

Findings

The jitter of the mobile robot caused by the drastic change of angular velocity is reduced when the robot is closer to the target point. The simulation results show that the proposed algorithm effectively optimizes the stability of the mobile robot during operation with lower angular velocity dispersion and less energy consumption, but with a slightly higher running time than DWA.

Originality/value

A novel enhanced dynamic window algorithm is proposed and verified. According to the experimental result, the proposed algorithm can reduce the energy consumption of the robot and improves the efficiency of the robot.
Are we talking about merger or acquisition? Defining the integration process
Authors: Thelisson Anne-Sophie
Published: August 2022

Purpose

When talking about mergers and acquisitions (M&As), few announcements clearly define if the operation will deal with a merger (where firms have an equal-to-equal relation) or acquisition (when one firm is in control of the operation and decides the integration process). Operations are commonly labeled M&A. Nevertheless, mergers remain rare, and the authors see that most of the time, operations designed and integrated with firms as equals end in the control of one of the entities over the other.

Design/methodology/approach

The authors investigate how two CEOs and their managers communicate during the due diligence period of a merger. The author describes the project merger of two French companies using longitudinal data.

Findings

This in-depth case study provides new insights into the due diligence period and the differences between M&As. The findings highlight how the decision to add an associate from a rival firm to the board ended the merger project as the situation evolved toward an acquisition in CEOs’ minds.

Research limitations/implications

The limitations are those concerning a single case study.

Practical implications

The paper highlights the complexity of merger negotiations and the unexpected events faced by stakeholders. The analysis, thus, contributes to an inclusive and integrative view of the challenges in the due diligence process, whereas first defining the operation as a merger or an acquisition is a first step in identifying the degree to which autonomy and interdependence will be given across firms, and how some strategic decisions will be implemented. This case study highlights two specific items that can be understood by managers as key elements in deal success: defining operations as a merger or an acquisition help internal and external stakeholders in planning the operation; leaving space for adjustment among partners engaged in negotiations during the due diligence period is also useful.

Social implications

Despite their frequency, merger and acquisition failures remain surprisingly high. This paper explores how stakeholders deal with merger negotiations.

Originality/value

The case provides insights into the due diligence period and the way minor events can impact the planned integration. Theoretical concepts and empirical findings from the literature are combined to present a single consistent picture. To the best of the authors’ knowledge, few studies address insights on strategic decisions made as the negotiation period remains a secret and sensitive stage, especially for a failed deal, but we were able to delve beneath the surface.
Exploring the spatial structure of Toon historical town courtyard houses: topological characteristics of the courtyard based on a configuration approach
Authors: Kamelnia Hamed , Hanachi Pirouz , Moayedi Mina
Published: August 2022

Purpose

In response, this study developed a mathematical and computational method, through spatial configurations with justified plan graph (JPG), drawn from Space Syntax to extract essential information of the spatial topology of 13 valuable traditional courtyard houses located in Toon historical city of Iran.

Design/methodology/approach

Historical vernacular housing has always been designed to incorporate and reflect the local lifestyle and cultural conditions. “Courtyard” is a fundamental part of traditional Iranian houses. This traditional building type includes a walled boundary and a complex of open, semi-enclosed and enclosed spaces. This study investigates the traditional houses in Toon historical town. Toon is one of the ancient towns in southeast Iran, including old courtyard houses in its heritage context. The spatial influence of the courtyard contributes to the formation of this famous architectural type.

Findings

The results show the remarkable impact of this space on dominating the plan of traditional Toon houses configuration compared to other spaces in all cases. Furthermore, spatial accessibility has changed over time, and the Safavid period had a higher level of integration and lower level of accessibility (mean i = 7.03) rather than the Qajar period (mean i = 6.34); also, privacy has decreased progressively.

Originality/value

The knowledge of characteristics of this historical architecture needs to be gathered for the preservation and conservation of the built heritage. Hence, the principle behind traditional Iranian courtyards was investigated to recognize the difference between the spatial influence of the courtyard and other spaces and the changes in the courtyard over time.
Following the data: an analysis of two schools' use of social emotional data
Authors: Roegman Rachel , Tan Kevin , Tanner Nathan , Yore Caitlin
Published: August 2022

Purpose

Drawing on Coburn and Turner's framework for research on data use, this study looks at how contextual factors support interactions around data. In so doing, the authors contribute to the emerging body of literature on administrators supporting high school students' social-emotional learning (SEL).

Design/methodology/approach

This two-site case study “follows the data” that were shared with administrators at two high schools based on a longitudinal study of students' SEL. One author of this study has been leading a research project of high school students' SEL in two high schools from two different districts in a Midwest university town since 2017. This study follows what happened in both high schools after the author shared students' SEL data with district personnel.

Findings

Findings showed that participants were invested in increasing SEL programming. However, SEL data moved in different ways through the two schools, and all individuals had different ideas about which data were important. Each district dealt with a specific set of organizational norms, existing inequities, and beliefs systems that influenced which SEL data were noticed and how, if at all, data spurred action.

Originality/value

Specific aspects of organizational contexts support and constrain SEL data use. Both cases suggest researchers can guide data use practices that can advance students' SEL. However, each district dealt with a specific set of educational inequities, which influenced which data were noticed and how, if at all, data spurred action. Importantly, data-driven decision-making must be conducted from an equity lens, lest the process replicate existing inequities.
The dynamic relationship between firms’ cash reserves and financial leverage: evidence from MENA emerging markets
Authors: Almustafa Hamza , Kalash Ismail
Published: August 2022

Purpose

This paper investigates the impact of financial leverage on corporate cash holdings in the Middle East and North African (MENA) emerging markets.

Design/methodology/approach

The author applies the dynamic modeling approach to data from nonfinancial firms listed in 10 MENA countries between 2010 and 2019. The empirical model avoids the shortcomings of the prior literature by including indicators of the dynamics of the financial leverage to account for its persistence in the corporate cash holdings reserves.

Findings

This research reports a significant negative relationship between corporate cash holdings and financial leverage. The results support the pecking order model, suggesting that leverage can be regarded as a substitute for holding a larger amount of cash and marketable securities. The author argues that the negative relationship between financial leverage and corporate cash holdings reinforces the precautionary motive to have internal cash reserves rather than external debt to support capital and investment activities by firms in the MENA emerging markets.

Practical implications

The results of this research provide important insights into cash and capital structure management for nonfinancial listed firms in the MENA emerging markets. Specifically, the paper will help managers to understand the dynamic financial leverage determinants of holding cash in corporations in the MENA emerging markets and encourage policymakers to financially determine the corporate capital structure and cash holdings based on cost and benefits. Managing the firm's capital structure and cash holdings based on trade-offs between costs and benefits would enhance operating cash flow which may play an important role in creating value for shareholders.

Originality/value

Prior studies have commonly been concerned with the determinants of corporate cash holdings, but few have investigated the dynamic financial leverage determinants of corporate cash holdings. This paper draws attention to this issue within the context of MENA emerging markets. To the authors' best knowledge, this is the first study that explores the relationship between cash holdings and financial leverage in MENA emerging markets.
Hospitals management transformative initiatives; towards energy efficiency and environmental sustainability in healthcare facilities
Authors: Dion Helen , Evans Martin , Farrell Peter
Published: August 2022

Purpose

Energy-saving is a growing challenge worldwide because of population growth, economic activity and high consumption rates that are unsustainable in the long term. Health-care facilities and hospitals face the challenge of increases in operational costs. This paper aims to appraise challenges to adopting energy-saving policies and proposes a roadmap for sustainability and energy efficiency management in hospitals and health-care facilities.

Design/methodology/approach

Eight hospitals were examined as case studies through qualitative interviews with hospital senior management, executives and health-care facilities managers in addition to collecting relevant data from the literature; there is critical appraisal and content analysis of this data.

Findings

This study established factors influencing implementation and challenges to energy-saving strategies. This study proposed guidelines for efficient energy management in hospitals and health-care facilities. This study concluded that the best performance is secured by integrating the proposed guidelines with the adoption of ISO 50001 energy management systems to achieve the United Nations’ sustainable development goal – SDG 7 “affordable and clean energy”.

Research limitations/implications

This study is limited to the initiatives/experiences of the hospitals studied in the Middle East and North Africa region.

Originality/value

This study’s findings, conclusions, recommendations and proposed guidelines enrich the body of knowledge. This will allow industry key stakeholders, hospitals and health-care facilities managers to overcome challenges of implementing energy management. In addition, adopting the proposed guidelines will improve energy efficiency and help hospitals in green initiatives as they seek to demonstrate their support for United Nations’ sustainable development goals.
Workplace coaching: testing whether personality traits and their ABCD components predict authentic self-development via affect balance
Authors: Erdös Tünde , Wilt Joshua , Tichelmann Michael
Published: August 2022

Purpose

Little is known about how individual differences play out in the process of authentic self-development (ASD) through workplace coaching. This article explores whether the Big Five personality traits and affective, behavioral, cognitive and desire (ABCDs) components of the Big Five personality traits were relevant to ASD, specifically examining the role of affect as a potential mediator.

Design/methodology/approach

In total, 176 clients' personality was assessed pre-coaching. Aspects of ASD (perceived competence, goal commitment, self-concordance and goal stability) were assessed post-coaching. Clients' affect balance (AB) scores were obtained post-session.

Findings

Multilevel path models showed that higher levels of mean AB (but not the slope) mediated the associations between personality and perceived competence and goal commitment. Personality predicted goal self-concordance, but these effects were not mediated by AB, neither personality nor AB predicted goal stability.

Research limitations/implications

The authors encourage randomized controlled trials to further test findings of this study. Ruling out method variance is not possible completely. However, the authors put forth considerations to support the authors' claim that method variance did not overly influence our results.

Practical implications

These results suggest the necessity of an optimal experience of affect for ASD in workplace coaching and the understanding of how ABCDs, AB and ASD are related beyond coaching psychology.

Social implications

A deeper understanding of personality processes is important for fostering ASD to meet the challenges of management development in the authors' volatility, uncertainty, complexity and ambiguity (VUCA) world.

Originality/value

This is the first study to test personality as a process in workplace coaching linking personality to one of the most valued leadership skills: authenticity.
Drugs or despair? Unraveling the rise in deaths of despair in Illinois
Authors: Cataldo Jessica
Published: August 2022

Purpose

Increases in deaths of despair in the USA have been associated with economic conditions and drug availability. In the state of Illinois, deaths of despair represent a significant public health issue. This study aims to examine the relationship between county-level economic distress, drug availability and mortality from deaths of despair collectively and for each contributing cause of death individually in the state of Illinois to better understand drivers of mortality locally.

Design/methodology/approach

Two cross-sectional analyses were conducted for 2010–2014 and 2015–2019. Correlations, regression analyses and relative weight analyses were applied to assess the relationship between deaths of despair mortality rate and the individual variables.

Findings

Deaths of despair mortality collectively and for each cause of death individually increased significantly from 2010–2014 to 2015–2019 in Illinois. Suicide mortality was higher in rural counties and was related to economic distress, while drug poisoning and alcohol-related deaths were higher in urban counties and were related to drug availability indicators.

Originality/value

While all three causes of death increased in the state, suicide mortality was inversely related to deaths of despair mortality. This may be because of different individual risk factors in rural versus urban areas or issues with coding cause of death. The findings of this study point to a rising public health challenge of deaths of despair mortality, particularly from substance use, in urban counties and from suicide in rural counties.
Use and perception of gerontechnology: differences in a group of Spanish older adults
Authors: Murciano-Hueso Alicia , Martín-Lucas Judith , Serrate González Sara , Torrijos Fincias Patricia
Published: August 2022

Purpose

The purpose of this paper is to understand the profile of use of gerontechnology in Spanish older adults considering their age group (60–70; 71–80 and over 80 years) and to discern whether groups of subjects with similar characteristics can be established to ascertain which factors are behind the profile of frequent gerontechnology use.

Design/methodology/approach

A quantitative study is presented to understand the profile of use of gerontechnology in Spanish older adults. The sample comprised 497 participants (aged between 60 and 94 years).

Findings

The results show that, even though most participants consider technology to be useful in their daily lives, there is still a lack of knowledge on how to use it, especially among older subjects. This highlights the importance of promoting technological cocreation initiatives such as senior living labs.

Research limitations/implications

Other researchers are encouraged to include the voices of older adults using gerontechnology in further studies.

Practical implications

If we want to increase the acceptance of technology by older adults, we must first let them take part in the design of the technologies they will use.

Social implications

This research provides promising data that should merit attention to contribute to the well-being and quality of life of older adults in a society where currently technology is a key part in every sphere of our daily life.

Originality/value

The value of this research lies in the implications of “aging in place” studies today.
Is teleworking at odds with social sustainability and organizational learning?
Authors: Mosquera Pilar , Soares Maria Eduarda , Alvadia Teresa
Published: August 2022

Purpose

The well-being of individuals is an essential issue of sustainability research. This study aims to focus on the particular case of teleworkers and the work conditions which may hinder their well-being. More specifically, this study analyses the impact of social isolation and work overload on the work engagement and life satisfaction of teleworkers during COVID-19 pandemic.

Design/methodology/approach

With a sample of 188 workers from the Portuguese banking sector, the authors use structural equations modelling to test a conceptual model. The authors carry out data analysis with partial least squares.

Findings

Social isolation and work overload are both negatively associated with the three dimensions of work engagement, namely, vigour, absorption and dedication. In turn, vigour is positively associated with life satisfaction. Social isolation and work overload are indirectly associated with life satisfaction through vigour. Results from multi-group analysis show that the negative relationship between social isolation and dedication is stronger for less senior teleworkers. By contrast, the negative relationship between work overload and dedication is stronger for more senior teleworkers. Additionally, the positive relationship between vigour and life satisfaction is stronger for permanent workers than for temporary ones.

Originality/value

This study tests the assumptions of the job demands-resources under the novel conditions of telework during COVID-19, focusing its ubiquitous conditions – social isolation and work overload. For human resources managers, this study highlights the need to take seniority and job stability in consideration when devising onboarding and working arrangement strategies for teleworkers.
Case series of modified dermis graft for skin defects of the nasal region
Authors: Segawa Yuichiro , Tono Hisayuki , Chiba Hiromu , Sato Yota , Asano Yoshihide
Published: August 2022

Local skin flap from the surrounding area is often chosen for reconstruction of skin defects in the nasal region, but it is problematic because it creates new facial scar and requires skill of design. On the other hand, full‐thickness skin graft is associated with problems such as color mismatch and scar contracture at the recipient site. We reconstructed nasal defects of seven patients using modified dermis graft technique. In this method, de‐epithelialized full‐thickness skin graft is transplanted and then epithelialized from the surrounding area. This is a simple and easy procedure without causing a new scar on the face and is aesthetically and functionally superior to a standard full‐thickness skin graft; therefore, it is a useful method of reconstruction of the nasal area.
Bottom‐up effects of plant quantity and quality on arthropod diversity across multiple trophic levels in a semi‐arid grassland
Authors: Lu Xiaoming , Zhao Xuezhen , Tachibana Taiki , Uchida Kei , Sasaki Takehiro , Bai Yongfei
Published: August 2022

Plant quantity and quality can independently affect the diversity of the entire arthropod communities and multiple arthropod taxa in grassland ecosystems. However, it remains unclear how these effects on arthropod taxa at one trophic level propagate through food web to influence the diversity of higher trophic levels.

We performed a monoculture experiment with 15 herbaceous species in the Inner Mongolian grassland to investigate how natural variations in plant productivity and host leaf traits affect herbivore taxon richness, which, in turn, affects predator taxon richness.

For herbivores, plant productivity indirectly promoted herbivore taxon richness by increasing herbivore biomass, which was attributed to the increases in the richness of dominant sucking herbivores and endophytes with high food requirements. However, the high plant quality indicator (e.g. high leaf protein, phosphorus and water contents, and high leaf protein to carbohydrate ratio) directly increased, whereas the low plant quality indicator (e.g. high leaf lignin content) directly decreased herbivore taxon richness. Taxon richness of chewing and sucking herbivores with specific feeding modes (tearing or sucking mouthparts) showed strong positive responses to increasing plant quality.

For predators, herbivore taxon richness, rather than herbivore biomass, mainly mediated the positive effects of plant productivity and the high plant quality indicator, but the negative effect of the low plant quality indicator, on predator taxon richness. At the feeding guild level, the taxon richness of parasitoids, other predators and spiders exhibited positive responses to different herbivores, which was attributed to their different diet preferences. Predator diversity could be promoted by prey partitioning among predator guilds facilitating species coexistence. At the family level, the taxon richness of most predator families was positively correlated with that of more than one herbivore family, suggesting that high predator diversity may be caused by balanced diets owing to high prey diversity.

Synthesis. Natural variations in plant quantity and quality can substantially affect the diversity of herbivores and cascade up the food web to affect predators. Specificity and mechanisms of feeding have a large impact on the responses of arthropod guilds at each trophic level.
Implementing local planetary health: Case study of Blue Mountains, Australia
Authors: Robson Eleanor , Crabtree‐Hayes Louise , Langevad William , Zhong Hua Flora , Barrett Kim , Salazar Juan Francisco
Published: August 2022

Planetary health is a concept that emerged from public health and is of central interest for geographers. Blue Mountains City Council (BMCC) launched its planetary health initiative in 2020, which is a first instance of it being adopted by an Australian local government as a sustainability policy framework. This article presents an initial case study arguing for and testing how to gain empirical insights from developing local planetary health initiatives. Using an online survey of Blue Mountains residents from 2020, we elucidate key opportunities and challenges associated with implementing planetary health in local contexts. We found that respondents experience the impacts of environmental change in varied ways and want to do more to address environmental change. They demand action from governments and others to act, yet do not understand well the mechanisms available to local governments to undertake such action. We also established that planetary health shows promise in its ability to accommodate diverse and scalar experiences and action from personal to communal. Thus, our key claim is that it could be an inclusive basis upon which local governments and communities can deliberate and make decisions about sustainability. However, local governments could experience difficulties in implementing planetary health should deliberation not improve community understanding of the levers available to local government to act on environmental change and the nature of shared responsibility for place‐based sustainability. We suggest geographers could do more work on such matters.
Adjuvant anticholinergic therapy for the prevention of akathisia in patients with primary headache in the emergency department: A systematic review
Authors: Meyer Jillian , Kirkland Scott W. , Campbell Sandra , Villa‐Roel Cristina , Rowe Brian H.
Published: August 2022

Objectives

Adjunct therapy with anticholinergic agents has been proposed to reduce the incidence of extrapyramidal side effects such as akathisia following treatment with neuroleptics or metoclopramide. This systematic review assessed the effectiveness of anticholinergic agents to prevent neuroleptic or metoclopramide‐induced akathisia in patients presenting to the emergency department (ED) with benign headache.

Methods

Eight electronic databases and the gray literature were searched to identify randomized controlled trials involving adult patients presenting to the ED with primary headache treated with neuroleptic or metoclopramide. Study selection, data extraction, and quality assessment were completed by two independent reviewers. Individual or pooled meta‐analysis of dichotomous outcomes were calculated as relative risks (RRs) with 95% confidence intervals (CIs) using a random‐effects model. Heterogeneity was assessed using theI2statistic.

Results

A total of 1032 studies were screened, of which two studies were included in the review. Both studies provided patients with diphenhydramine following treatment with neuroleptics or metoclopramide. Treatment with diphenhydramine did not reduce the incidence of akathisia compared to treatment with placebo (RR 0.83, 95% CI 0.43–1.61,I2 = 0%). The impact of diphenhydramine on pain relief, need for rescue medications, and relief of other extrapyramidal side effects was reported in one of the two studies, with no significant differences noted in any outcomes compared to patients treated with placebo.

Conclusion

This review found insufficient evidence to recommend the use of diphenhydramine as an adjunct therapy to prevent akathisia in ED patients treated with neuroleptics or metoclopramide for primary headache. This finding relies on the results of two small randomized controlled trials with incomplete outcome reporting. Additional high‐quality studies are needed to better understand the clinical efficacy of agents with anticholinergic properties in the ED management of patients with primary headaches.