bluuebunny/crossref_metadata_2025 huggingface dataset:
The prevalence and determinants of blood pressure control among hypertension patients in eastern Zimbabwe: A cross-sectional study
Authors: Mundagowa Paddington Tinashe , Zambezi Pemberai , Muchemwa-Munasirei Priscillah
Published: March 2024

Background

Uncontrolled blood pressure (BP), also known as hypertension, is a leading cause of morbidity and mortality globally. Lowering the elevated BP can significantly reduce one’s risk for cardiovascular diseases. This study aimed to ascertain the determinants of BP control among hypertension patients.

Methods

The data analyzed were from the exploratory survey of the Home Management of Hypertension (HoMHyper) project in eastern Zimbabwe. Hypertension patients were selected from the Chronic Disease Registers of five public health clinics using simple random sampling. A pretested interviewer-administered questionnaire was used to collect data, and the patient’s BP was measured. The primary outcome, BP control, was used as a categorical variable (controlled vs. uncontrolled) to conduct a bivariate analysis. Variables significant at p<0.2 were included in the multivariable logistic regression analysis to control for confounding. Statistical significance in the final model was set at p<0.05.

Results

Data from 321 hypertension patients were analyzed; their mean age was 62.3±11.9 years. The prevalence of controlled BP was 41.4% (95% Confidence interval-CI = 36.0%-46.9%). After adjusting for confounding, patients’ residence and medication stocks were associated with BP control. Patients who resided in high-density suburbs had higher odds of uncontrolled BP than those who resided in middle- and low-density suburbs (Adjusted odds ratios-AOR = 2.5; 95% CI = 1.4–4.4; p<0.01). Hypertension patients who experienced medication stockouts over the last six months had higher odds of uncontrolled BP than patients who did not experience stockouts (AOR = 1.8; 95% CI = 1.1–2.9).

Conclusion

BP control among hypertension patients was suboptimal. Patient residence and antihypertensive medication stockouts were independent predictors of blood pressure control. We recommend exploring sustainable financing through private-public partnerships to ensure the availability of subsidized antihypertensive medication.
A short-term price prediction-based trading strategy
Authors: Fan Weijia , Zhang Ru , He Hao , Hou Siyu , Tan Yongbo
Published: March 2024

Quantitative investment theory has emerged as a prominent and widely researched domain within the financial markets, where investors predominantly focus on discerning the intricate influences of market dynamics. In this paper, we proposed a short-term prediction-based trading strategy, which can equiponderate between return and risk, considerations while accounting for investor risk preferences. This strategy employs GM(1,1) to capture nuanced features of price dynamics in short-term intervals and update the GM(1,1) model with the latest data. Subsequently, a multi-objective planning equation is formulated to optimize asset allocations by determining the optimal holding that strikes between specific returns and risk mitigation. In the end, this work conducts a case study and sensitivity analysis using five years of gold and bitcoin price data spanning from 2016 to 2021. This empirical examination serves to affirm the efficacy and resilience of the proposed trading strategy. The case study reveals that proficient short-term price forecasting serves as a potent means to proactively mitigate risk, facilitating, judicious and objective trading practices. Moreover, it underscores the strategy’s tangible utility as a guide for real-world investment decisions.
Comparison of five different disseminated intravascular coagulation criteria in predicting mortality in patients with sepsis
Authors: Zafar Amara , Naeem Filza , Khalid Muhammad Zain , Awan Safia , Riaz Muhammad Mehmood , Mahmood Saad Bin Zafar
Published: March 2024

Objective

Even though patients with sepsis and DIC have a higher mortality rate compared to those without DIC, screening for DIC is not currently part of sepsis management protocols. This may be due to a lack of literature on the frequency of DIC occurrence in sepsis patients, as well as the absence of evidence on the optimal DIC criteria to use for identifying DIC and predicting mortality among the five criteria available. To address this gap, this study investigates the predictive value of five different criteria for diagnosing DIC and its relationship to patient outcomes in our population of sepsis patients.

Methods

In the Medicine department of Aga Khan University Hospital, a retrospective observational study was conducted, enrolling all adult patients with International Classification of Diseases, 9th Revision, Clinical Modification (ICD-9-CM) coding of sepsis and clinical suspicion of DIC between January 2018 and December 2020. To diagnose DIC, five different criteria were utilized, namely the International Society of Thrombosis and Hemostasis (ISTH), the Korean Society on Thrombosis and Hemostasis (KSTH), the Japanese Association for Acute Medicine (JAAM), the revised-JAAM (RJAAM), and the Japanese Ministry of Health and Welfare (JMHW). The study analyzed the sensitivity, specificity, negative predictive value, positive predictive value, and accuracy of these five criteria, as well as the overall prediction of mortality.

Results

Of 222 septic patients included in this study with clinical suspicion of DIC, 94.6% of patient had DIC according to KSTH criteria, followed by JAAM (69.4%), ISTH (64.0%), JMHW (53.2%) and lastly R-JAAM (48.6%). KSTH had sensitivity of 95.4% in diagnosing DIC and predicting mortality with a positive predictive value of 70% but specificity of 7.3% only. JAAM had sensitivity of 75.9%, positive predictive value of 75.9% with a specificity of 45.5%. ISTH had sensitivity of 69.4%, positive predictive value 75.3% and specificity of 48.5%.

Conclusion

DIC can impose a significant burden on septic patients and its presence can lead to higher mortality rates. Early detection through screening for DIC in septic patients can potentially reduce mortality. However, it is necessary to identify the most appropriate diagnostic criteria for each population, as various criteria have demonstrated different performance in different populations. Establishing a gold standard for each population can aid in accurate diagnosis of DIC.
Stable demographic ratios of haploid gametophyte to diploid sporophyte abundance in macroalgal populations
Authors: Bessho Kazuhiro
Published: March 2024

Macroalgal populations often consist of free-living haploid (gametophyte) and diploid (sporophyte) stages. Various ecological studies have been conducted to examine the demographic diversity of haploid-diploid populations with regard to the dominant stage. Here, I relaxed the assumption of classical research that the life history parameters of haploids and diploids are identical and developed a generalized haploid-diploid model that explicitly accounts for population density dependence and asexual reproduction. Analysis of this model yielded an exact solution for the abundance ratio of haploids to diploids in a population in which the ratio is determined by the balance of four demographic forces: sexual reproduction by haploids, sexual reproduction by diploids, asexual reproduction by haploids, and asexual reproduction by diploids. Furthermore, the persistence of a haploid-diploid population and its total biomass are shown to be determined by the basic reproductive number (R0), which is shown to be a function of these four demographic forces. When R0 is greater than one, the haploid-diploid population stably persists, and the ploidy ratio obtained by the analytical solution is realized.
Gene expression profiling of Jack Pine (Pinus banksiana) under copper stress: Identification of genes associated with copper resistance
Authors: Moy Alistar , Czajka Karolina , Michael Paul , Nkongolo Kabwe
Published: March 2024

Understanding the genetic response of plants to copper stress is a necessary step to improving the utility of plants for environmental remediation and restoration. The objectives of this study were to: 1) characterize the transcriptome of Jack Pine (Pinus banksiana) under copper stress, 2) analyze the gene expression profile shifts of genotypes exposed to copper ion toxicity, and 3) identify genes associated with copper resistance. Pinus banksiana seedlings were treated with 10 mmoles of copper and screened in a growth chamber. There were 6,213 upregulated and 29,038 downregulated genes expressed in the copper resistant genotypes compared to the susceptible genotypes at a high stringency based on the false discovery rate (FDR). Overall, 25,552 transcripts were assigned gene ontology. Among the top upregulated genes, the response to stress, the biosynthetic process, and the response to chemical stimuli terms represented the highest proportion of gene expression for the biological processes. For the molecular function category, the majority of expressed genes were associated with nucleotide binding followed by transporter activity, and kinase activity. The majority of upregulated genes were located in the plasma membrane while half of the total downregulated genes were associated with the extracellular region. Two candidate genes associated with copper resistance were identified including genes encoding for heavy metal-associated isoprenylated plant proteins (AtHIP20 and AtHIP26) and a gene encoding the pleiotropic drug resistance protein 1 (NtPDR1). This study represents the first report of transcriptomic responses of a conifer species to copper ions.
Chronic intermittent hypoxia aggravated diabetic cardiomyopathy through LKB1/AMPK/Nrf2 signaling pathway
Authors: Liu Bingbing , Si Jianchao , Qi Kerong , Li Dongli , Li Tingting , Tang Yi , Ji Ensheng , Yang Shengchang
Published: March 2024

Chronic intermittent hypoxia (CIH) may play an important role in the development of diabetic cardiomyopathy (DCM). However, the exact mechanism of CIH-induced myocardial injury in DCM remains unclear. In vivo, the db/db mice exposed to CIH were established, and in vitro, the H9C2 cells were exposed to high glucose (HG) combined with intermittent hypoxia (IH). The body weight (BW), fasting blood glucose (FBG) and food intake were measured every two weeks. The glycolipid metabolism was assessed with the oral glucose tolerance test (OGTT) and insulin resistance (IR). Cardiac function was detected by echocardiography. Cardiac pathology was detected by HE staining, Masson staining, and transmission electron microscopy. The level of reactive oxygen species (ROS) in myocardial tissue was detected by dihydroethidium (DHE). The apoptosis was detected by TUNEL staining. The cell viability, ROS, and the mitochondrial membrane potential were detected by the cell counting kit-8 (CCK-8) assay and related kits. Western blotting was used to analyze the liver kinase B1/AMP-activated protein kinase/ nuclear factor-erythroid 2-related factor 2 (LKB1/AMPK/Nrf2) signaling pathway. CIH exposure accelerated glycolipid metabolism disorders and cardiac injury, and increased the level of cardiac oxidative stress and the number of positive apoptotic cells in db/db mice. IH and HG decreased the cell viability and the level of mitochondrial membrane potential, and increased ROS expression in H9C2 cells. These findings indicate that CIH exposure promotes glycolipid metabolism disorders and myocardial apoptosis, aggravating myocardial injury via the LKB1/AMPK/Nrf2 pathway in vitro and in vivo.
Influence of type of violence on women’s help-seeking behaviour: Evidence from 10 countries in sub-Saharan Africa
Authors: Ayebeng Castro , Dickson Kwamena Sekyi , Ameyaw Edward Kwabena , Adde Kenneth Setorwu , Paintsil Jones Arkoh , Yaya Sanni
Published: March 2024

Background

Intimate partner violence (IPV) is a major public health concern that mostly impacts women’s health and social well-being. This study explored how the various types of IPV (physical, sexual, and emotional) including women’s experience of childhood violence influence their help-seeking behavior in sub-Saharan Africa (SSA).

Methods

We analyzed data from the most recent Demographic and Health Surveys (DHS), carried out between 2018 and 2021. The outcome variable was help-seeking behavior. Descriptive and inferential analyses were carried out. The descriptive analysis looked at the bivariate analysis between the country and outcome variables. Using a binary logistic regression model, a multivariate analysis was utilized to determine the association between the outcome variable and the explanatory variables. Binary logistic regression modelling was used based on the dichotomous nature of the outcome variable. The results were sample-weighted to account for any under- or over-sampling in the sample.

Results

The proportion of women who sought help for intimate partner violence was 36.1 percent. This ranged from 19.2 percent in Mali to 49.6 percent in Rwanda. Women who experienced violence in childhood (OR = 0.75, CI = 0.69, 0.82) have a lower likelihood of seeking help compared to those who did not experience violence in their childhood. Women who had experienced emotional violence (OR = 1.94, CI = 1.80, 2.08), and physical violence (OR = 1.37, CI = 1.26, 1.48) have a higher likelihood of seeking help compared to those who have not. Women with secondary educational levels (aOR = 1.13, CI = = 1.02, 1.24) have a higher likelihood of seeking help compared to those with no education. Cohabiting women have a higher likelihood (aOR = 1.22, CI = 1.10, 1.35) of seeking help compared to married women.

Conclusion

The study highlights the importance of early identification of IPV and fit-for-purpose interventions to demystify IPV normalization to enhance women’s willingness to seek help. The study’s findings suggest that education is crucial for increasing women’s awareness of the legalities surrounding IPV and available structures and institutions for seeking help.
Morphological differentiation of peritumoral brain zone microglia
Authors: Salas-Gallardo G. Anahí , Lorea-Hernández Jonathan-Julio , Robles-Gómez Ángel Abdiel , Del Campo Claudia Castillo-Martin , Peña-Ortega Fernando
Published: March 2024

The Peritumoral Brain Zone (PBZ) contributes to Glioblastoma (GBM) relapse months after the resection of the original tumor, which is influenced by a variety of pathological factors. Among those, microglia are recognized as one of the main regulators of GBM progression and probably relapse. Although microglial morphology has been analyzed inside GBM and its immediate surroundings, it has not been objectively characterized throughout the PBZ. Thus, we aimed to perform a thorough characterization of microglial morphology in the PBZ and its likely differentiation not just from the tumor-associated microglia but from control tissue microglia. For this purpose, Sprague Dawley rats were intrastriatally implanted with C6 cells to induce a GBM formation. Gadolinium-based magnetic resonance imaging (MRI) was performed to locate the tumor and to define the PBZ (2 mm beyond the tumor border), thus delimitating the different regions of interest (ROIs: core tumoral zone and immediate interface; contralateral striatum as control). Brain slices were obtained and immunolabeled with the microglia marker Iba-1. Sixteen morphological parameters were measured for each cell, significative differences were found in all parameters when comparing the four ROIs. To determine if PBZ microglia could be morphologically differentiated from microglia in other ROIs, hierarchical clustering analysis was performed, revealing that microglia can be separated into four morphologically differentiated clusters, each of them mostly integrated by cells sampled in each ROI. Furthermore, a classifier based on linear discriminant analysis, including only three morphological parameters, categorized microglial cells across the studied ROIs and showed a gradual transition between them. The robustness of this classification was assessed through principal component analysis with the remaining 13 morphological parameters, corroborating the obtained results. Thus, in this study we provided objective and quantitative evidence that PBZ microglia represent a differentiable microglial morphotype that could contribute to the recurrence of GBM in this area.
The impact of performance feedback on corporate ESG performance: Mediating role of environmental strategy
Authors: Ren Changman , Lin Xiaoxing
Published: March 2024

Purpose

The purpose of this study is to investigate the impact of performance feedback (performance expectation surplus, performance expectation deficit) on corporate ESG performance, and this paper also to investigate the role of environmental strategy as a mechanism in the impact of enterprises’ performance feedback on corporate ESG performance.

Design/Methodology/Approach

The study used data from 3679 companies listed on the Shanghai and Shenzhen stock exchanges for the period 2009–2021 and also measured the intensity of corporate environmental strategies through analysis. Finally, we used a fixed effects model to test the research hypothesis.

Findings

This study shows that enterprise performance feedback positively affects corporate ESG performance and that environmental strategy plays a significant mechanistic role in enterprise performance feedback and corporate ESG performance. Overall, performance expectation surplus negatively affects ESG performance, performance expectation deficit positively affects ESG performance, and the mechanism of environmental strategy plays a significant role in performance expectation deficit and ESG performance.

Practical implications

The results of this study can help enterprises establish a scientific environmental management system, strengthen the supervision of enterprise environmental management, and have certain reference significance for enterprises to speed up the implementation of environmental protection measures.

Originality/Value

This study adds to the literature by describing corporate ESG performance using performance feedback theory and explaining the inherent role of enterprise performance feedback in corporate ESG performance utilizing environmental strategies.
Protocol for creating a dataset of U.S. state alcohol-related firearm laws 2000–2022
Authors: Silver Diana , Bae Jin Yung , Macinko James
Published: March 2024

Firearms are a major source of preventable morbidity and mortality in the United States, contributing to over 48,000 deaths in 2022 and generating societal costs in excess of $500 billion. A body of work has examined the relationship between US state level firearm laws and health outcomes, generally finding that some firearm regulations are associated with lower firearm-related mortality. Alcohol has been identified as an additional risk factor for both homicides and suicide and stronger state alcohol laws have been associated with lower rates of suicide. To date, there are no empirical studies that have investigated the impact of laws over a long period of time that target the intersection of alcohol and firearm. One reason for this may be because there is no existing dataset that includes the range of these state laws over time. This study describes the protocol for collecting, coding and operationalizing these legal data.
Multi-angle laser device improves novice learning of C-arm fluoroscopy for lumbar spine surgery
Authors: Zhuang Yuan-Dong , Li Rui-Jin , Wu Jia-Jun , He Xue-Wei , Zou Wen-Bin , Xu Xu-Chu , Lu Si-Qi , Chen Chun-Mei
Published: March 2024

Purpose

This study aims to evaluate the efficacy and satisfaction of using a multi-angle laser device (MLD) for C-arm fluoroscopy to assist novice learners during lumbar spine surgery.

Methods

Forty novice learners were randomly assigned to Group A using an MLD-equipped C-arm or Group B using a traditional C-arm. Both groups performed X-ray fluoroscopy on a lumbar spine model in supine and rotated positions. Time, number of shots, and deviation from the target were compared. A questionnaire was used to assess the learning experience.

Results

Group A required less time (13.66 vs. 25.63 min), and fewer shots (15.05 vs. 32.50), and had a smaller deviation (22.9% vs. 61.5%) than Group B (all p<0.05). The questionnaire revealed higher scores in Group A for comfort, efficiency, and knowledge mastery (all p<0.05).

Conclusion

The MLD significantly improves novice learning of C-arm fluoroscopy during lumbar spine surgery.
Detection of nitric oxide-mediated metabolic effects using real-time extracellular flux analysis
Authors: Vagher Bay , Amiel Eyal
Published: March 2024

Dendritic cell (DC) activation is marked by key events including: (I) rapid induction and shifting of metabolism favoring glycolysis for generation of biosynthetic metabolic intermediates and (II) large scale changes in gene expression including the upregulation of the antimicrobial enzyme inducible nitric oxide synthase (iNOS) which produces the toxic gas nitric oxide (NO). Historically, acute metabolic reprogramming and NO-mediated effects on cellular metabolism have been studied at specific timepoints during the DC activation process, namely at times before and after NO production. However, no formal method of real time detection of NO-mediated effects on DC metabolism have been fully described. Here, using Real-Time Extracellular Flux Analysis, we experimentally establish the phenomenon of an NO-dependent mitochondrial respiration threshold, which shows how titration of an activating stimulus is inextricably linked to suppression of mitochondrial respiration in an NO-dependent manner. As part of this work, we explore the efficacy of two different iNOS inhibitors in blocking the iNOS reaction kinetically in real time and explore/discuss parameters and considerations for application using Real Time Extracellular Flux Analysis technology. In addition, we show, the temporal relationship between acute metabolic reprogramming and NO-mediated sustained metabolic reprogramming kinetically in single real-time assay. These findings provide a method for detection of NO-mediated metabolic effects in DCs and offer novel insight into the timing of the DC activation process with its associated key metabolic events, revealing a better understanding of the nuances of immune cell biology.
A scoping review on the clinical effectiveness of Trans-Impedance Matrix (TIM) measurements in detecting extracochlear electrodes and tip fold overs in Cochlear Ltd devices
Authors: Ayas Muhammed , Muzaffar Jameel , Borsetto Daniele , Eitutis Susan , Phillips Veronica , Tam Yu Chuen , Salorio-Corbetto Marina , Bance Manohar L.
Published: March 2024

Background

Extrusion of electrodes outside the cochlea and tip fold overs may lead to suboptimal outcomes in cochlear implant (CI) recipients. Intraoperative measures such as Trans-Impedance Matrix (TIM) measurements may enable clinicians to identify electrode malposition and direct surgeons to correctly place the electrode array during surgery.

Objectives

To assess the current literature on the effectiveness of TIM measurements in identifying extracochlear electrodes and tip fold overs.

Methods

A scoping review of studies on TIM-based measurements were carried out using the Databases-Medline/PubMed, AMED, EMBASE, CINAHL and the Cochrane Library following PRISMA guidelines. Eleven full texts articles met the inclusion criteria. Only human studies pertaining to TIM as a tool used in CI were included in the review. Further, patient characteristics, electrode design, and TIM measurement outcomes were reported.

Results

TIM measurements were available for 550 implanted ears with the subjects age ranged between 9 months to 89 years. Abnormal TIM measurements were reported for 6.55% (36). Tip fold over was detected in 3.64% (20) of the cases, extracochlear electrodes in 1.45% (8), and 1.45% (8) were reported as buckling. Slim-modiolar electrode array designs were more common (54.71%) than pre-curved (23.34%) or lateral wall (21.95%) electrode array. Abnormal cochlear anatomy was reported for five ears (0.89%), with normal cochlear anatomy for all other patients.

Conclusion

TIM measurement is a promising tool for the intraoperative detection of electrode malposition. TIM measurement has a potential to replace intraoperative imaging in future. Though, TIM measurement is in its early stages of clinical utility, intuitive normative data sets coupled with standardised criteria for detection of abnormal electrode positioning would enhance its sensitivity.
New in Town—An internet-based self-efficacy intervention for internal migrants: A randomized controlled trial
Authors: Maj Anna , Matynia Maria , Michalak Natalia , Bis Aleksandra , Andersson Gerhard
Published: March 2024

Objective

Migration is a profound life transition that may threaten migrants’ well-being and mental health. Results of several studies suggest that social self-efficacy beliefs may be beneficial for the psychological adjustment of migrants, buffering the effect of specific stressors related to migration, helping them reduce anxiety levels, and providing support in forming of new social bonds and better integration with a new community or culture. The primary purpose of this randomized controlled trial was to examine the effectiveness of the New in Town internet-based self-efficacy intervention for internal migrants in Poland.

Methods

Participants were 158 internal adult migrants who had changed residence in the last 6 months. They were randomized into two groups: an experimental group (receiving an internet-based self-efficacy intervention), and a waiting list control group. We examined if the intervention was effective in enhancing participants’ social self-efficacy (primary outcome), general self-efficacy, social support, satisfaction with life, and reduced reported loneliness (secondary outcomes). Outcome measures were assessed at baseline (Time 1) and 3-weeks later (Time 2). The dropout rate was 50.6%. Initially, we planned to gather follow-up data also 8-weeks after baseline (Time 3). However, due to health and safety reasons related to the COVID-19 pandemic, we decided to stop the trial. Finally, we included in our analysis only data gathered before the COVID-19 pandemic at Time 1 and Time 2.

Results

A total of 159 individuals who met the study’s inclusion criteria and completed the baseline assessment were randomly assigned to either the experimental group (n = 80) or the waiting list control group (n = 79). Nevertheless, one participant assigned to the control group was excluded from the analyses because they withdrew their consent to participate after being randomized. The study results suggest that compared to the waitlist control group (n = 78), participants in the experimental group (n = 80) reported a higher level of general self-efficacy beliefs at Time 2 (Cohen’s d = 0.47; 95% CI: 0.15–0.79). However, there were no statistically significant effects on social self-efficacy, social support, satisfaction with life, and loneliness.

Conclusion

The study offers preliminary support for the effectiveness of an internet-based self-efficacy intervention designed for internal migrants on general self-efficacy beliefs.

Trial registration

The trial was registered with ClinicalTrials.gov (identifier: NCT04088487) on 11th September 2019.
Regional economic forecast using Elman neural networks with wavelet function
Authors: Liang Huade , Zeng Huilin , Dong Xiaojuan
Published: March 2024

Recently, the economy in Guangdong province has ranked first in the country, maintaining a good growth momentum. The prediction of Gross Domestic Product (GDP) for Guangdong province is an important issue. Through predicting the GDP, it is possible to analyze whether the economy in Guangdong province can maintain high-quality growth. Hence, to accurately forecast the economy in Guangdong, this paper proposed an Elman neural network combining with wavelet function. The wavelet function not only stimulates the forecast ability of Elman neural network, but also improves the convergence speed of Elman neural network. Experimental results indicate that our model has good forecast ability of regional economy, and the forecast accuracy reach 0.971. In terms of forecast precision and errors, our model defeats the competitors. Moreover, our model gains advanced forecast results to both individual economic indicator and multiple economic indicators. This means that our model is independently of specific scenarios in regional economic forecast. We also find that the investment in education has a major positive impact on regional economic development in Guangdong province, and the both surges positive correlation. Experimental results also show that our model does not exhibit exponential training time with the augmenting of data volume. Consequently, we propose that our model is suitable for the prediction of large-scale datasets. Additionally, we demonstrate that using wavelet function gains more profits than using complex network architectures in forecast accuracy and training cost. Moreover, using wavelet function can simplify the designs of complexity network architectures, reducing the training parameter of neural networks.
Effect of target gene sequence evenness and dominance on real-time PCR quantification of artificial sulfate-reducing microbial communities
Authors: Du Zhe , Behrens Sebastian F.
Published: March 2024

Quantitative real-time PCR of phylogenetic and functional marker genes is among the most commonly used techniques to quantify the abundance of microbial taxa in environmental samples. However, in most environmental applications, the approach is a rough assessment of population abundance rather than an exact absolute quantification method because of PCR-based estimation biases caused by multiple factors. Previous studies on these technical issues have focused on primer or template sequence features or PCR reaction conditions. However, how target gene sequence characteristics (e.g., evenness and dominance) in environmental samples affect qPCR quantifications has not been well studied. Here, we compared three primer sets targeting the beta subunit of the dissimilatory sulfite reductase (dsrB) to investigate qPCR quantification performance under different target gene sequence evenness and dominance conditions using artificial gBlock template mixtures designed accordingly. Our results suggested that the qPCR quantification performance of all tested primer sets was determined by the comprehensive effect of the target gene sequence evenness and dominance in environmental samples. Generally, highly degenerate primer sets have equivalent or better qPCR quantification results than a more target-specific primer set. Low template concentration in this study (~105 copies/L) will exaggerate the qPCR quantification results difference among tested primer sets. Improvements to the accuracy and reproducibility of qPCR assays for gene copy number quantification in environmental microbiology and microbial ecology studies should be based on prior knowledge of target gene sequence information acquired by metagenomic analysis or other approaches, careful selection of primer sets, and proper reaction conditions optimization.
Drug therapy problems and contributing factors among patients with epilepsy
Authors: Niriayo Yirga Legesse , Gebregziabher Tesfay , Demoz Gebre Teklemariam , Tesfay Nigusse , Gidey Kidu
Published: March 2024

Background

Although antiseizure medications play a crucial role in the management of epilepsy, their benefit can be compromised due to drug-related problems. Drug therapy problems can lead to poor seizure control, reduced quality of life, and increased morbidity and mortality in patients with epilepsy. However, in our setting, there is limited knowledge about drug therapy problems and the factors that contribute to them.

Objective

The aim of this study was to investigate the prevalence and contributing factors of drug-therapy problems among patients with epilepsy.

Methodology

A hospital-based prospective observational study was conducted at the neurologic clinic of Ayder Comprehensive Specialized Hospital, located in the Tigray region of Northern Ethiopia. The study included adult patients diagnosed with epilepsy who had been taking at least one antiseizure medication for a minimum of six months. Data were collected by conducting patient interviews and expert reviews of medical and medication records. Prior to data review and interviews, each patient provided written informed consent. Drug therapy problems were identified and classified using Cipolle’s method, followed by a consensus review conducted with a panel of experts. Statistical analysis was performed using a statistical software package; SPSS version 22. Binary logistic regression analysis was conducted to determine the contributing factors of drug therapy problems. Statistical significance was determined at p<0.05.

Results

A study conducted on 250 participants revealed that 55.2% of the patients experienced one or more drug therapy problems. Our analysis identified a total of 282 drug therapy problems, with a mean of 2±0.52 drug therapy problems per patient. The most commonly observed drug therapy problems were dosage too low (30.0%), noncompliance (22%), adverse drug reaction (18%), and unnecessary drug therapy (16.4%). The commonly involved antiseizure medications in these drug therapy problems were phenytoin (22.8%), Valproic acid (20.8%), and Phenobarbital (18.4%). Furthermore, our findings revealed that combination therapy (AOR: 3.92, 95%CI: 1.19–12.97) and uncontrolled seizure (AOR: 108.37, 95%CI: 38.7–303.6) exhibited significant associations with drug therapy problems.

Conclusion

Drug therapy problems were prevalent among patients with epilepsy. The use of combination therapy and the presence of uncontrolled seizures were identified as significant indicators of drug therapy problems. Therefore, more emphasis should be given to patients with multiple medications and uncontrolled seizures.
The effects of a synthetic epidermis spray on secondary intention wound healing in adult horses
Authors: Charlotte C. Paindaveine , Benoit Bihin , Olivier M. Lepage
Published: March 2024

Objectives

To evaluate secondary intention wound healing in the horse’s limbs when treated with the synthetic epidermis spray (Novacika®, Cohesive S.A.S, France) or with a standard bandaging technique.

Methods

Six Standardbred mares were included in the study. Four 2.5 x 2.5 cm full-thickness skin wounds were created on each thoracic limb. Two wounds were located on the dorsoproximal aspect of the cannon bone and the other two at the dorsoproximal aspect of the fetlock. Six hours after creation, wounds were randomly treated with synthetic epidermis spray or standard bandaging. The wounds were assessed every 4 days by gross visual assessment and using a 3D imaging camera. Analysis was performed with a 3D imaging application.

Results

Out of 46 wounds, 22 showed exuberant granulation tissue and were part of the standard bandaging group. Whether the wounds were treated with synthetic epidermis spray or standard bandaging, the time for healing was the same.

Conclusion

The synthetic epidermis spray studied in this model has allowed healing without the production of exuberant granulation tissue but did not reduce the median wound healing time compared to a standard bandaging technique. The synthetic epidermis spray is potentially an interesting alternative for the management of secondary intention wound healing of superficial and non-infected distal limb wounds in adult horses on economical and practical aspects. However, all statistical inference (p-values especially) must be interpreted with caution, given the size of the sample.
Coping strategies of psychiatrists and psychiatry trainees following patient suicide and suicide attempt: A national cross-sectional study in Saudi Arabia
Authors: Alawad Moayad S. , Alammari Mohammed A. , Almanea Mohannad M. , Alhumaid Rayan S. , Alkhalifah Azzam S. , Alosaimi Fahad D.
Published: March 2024

A patient’s suicide or suicide attempt is a challenging experience for psychiatrists. This study aimed to explore the common coping strategies and habits developed by psychiatrists/trainees following such incidents. A self-administered questionnaire was distributed among participants in Saudi Arabia. The study enrolled 178 participants, of whom 38.8% experienced a patient’s suicide, 12.9% experienced a patient’s severe suicide attempt, and 48.3% did not encounter any suicidal events. The most frequently utilized sources of support were colleagues (48.9%), team discussions (41.3%), and supervisors (29.3%). Only 21.4% received formal education in coping with a patient’s suicide. Approximately 94.9% reported a lack of support systems within their institution. The study highlighted the coping strategies most commonly employed by psychiatrists/trainees and revealed that the majority of participants reported no changes in their daily habits. The findings underscore the need for a structured support system and formal educational resources to address the existing deficit. Mental health organizations must take action to ensure adequate resources for healthcare providers.
Genome-wide analysis of MADS-box transcription factor gene family in wild emmer wheat (Triticum turgidum subsp. dicoccoides)
Authors: Mirzaghaderi Ghader
Published: March 2024

The members of MADS-box gene family have important roles in regulating the growth and development of plants. MADS-box genes are highly regarded for their potential to enhance grain yield and quality under shifting global conditions. Wild emmer wheat (Triticum turgidum subsp. dicoccoides) is a progenitor of common wheat and harbors valuable traits for wheat improvement. Here, a total of 117 MADS-box genes were identified in the wild emmer wheat genome and classified to 90 MIKCC, 3 MIKC*, and 24 M-type. Furthermore, a phylogenetic analysis and expression profiling of the emmer wheat MADS-box gene family was presented. Although some MADS-box genes belonging to SOC1, SEP1, AGL17, and FLC groups have been expanded in wild emmer wheat, the number of MIKC-type MADS-box genes per subgenome is similar to that of rice and Arabidopsis. On the other hand, M-type genes of wild emmer wheat is less frequent than that of Arabidopsis. Gene expression patterns over different tissues and developmental stages agreed with the subfamily classification of MADS-box genes and was similar to common wheat and rice, indicating their conserved functionality. Some TdMADS-box genes are also differentially expressed under drought stress. The promoter region of each of the TdMADS-box genes harbored 6 to 48 responsive elements, mainly related to light, however hormone, drought, and low-temperature related cis-acting elements were also present. In conclusion, the results provide detailed information about the MADS-box genes of wild emmer wheat. The present work could be useful in the functional genomics efforts toward breeding for agronomically important traits in T. dicoccoides.
Modulation of nucleotide metabolism by picornaviruses
Authors: Nouwen Lonneke V. , Breeuwsma Martijn , Zaal Esther A. , van de Lest Chris H. A. , Buitendijk Inge , Zwaagstra Marleen , Balić Pascal , Filippov Dmitri V. , Berkers Celia R. , van Kuppeveld Frank J. M.
Published: March 2024

Viruses actively reprogram the metabolism of the host to ensure the availability of sufficient building blocks for virus replication and spreading. However, relatively little is known about how picornaviruses—a large family of small, non-enveloped positive-strand RNA viruses—modulate cellular metabolism for their own benefit. Here, we studied the modulation of host metabolism by coxsackievirus B3 (CVB3), a member of the enterovirus genus, and encephalomyocarditis virus (EMCV), a member of the cardiovirus genus, using steady-state as well as 13C-glucose tracing metabolomics. We demonstrate that both CVB3 and EMCV increase the levels of pyrimidine and purine metabolites and provide evidence that this increase is mediated through degradation of nucleic acids and nucleotide recycling, rather than upregulation of de novo synthesis. Finally, by integrating our metabolomics data with a previously acquired phosphoproteomics dataset of CVB3-infected cells, we identify alterations in phosphorylation status of key enzymes involved in nucleotide metabolism, providing insight into the regulation of nucleotide metabolism during infection.
Multiple genetic loci influence vaccine-induced protection against Mycobacterium tuberculosis in genetically diverse mice
Authors: Kurtz Sherry L. , Baker Richard E. , Boehm Frederick J. , Lehman Chelsea C. , Mittereder Lara R. , Khan Hamda , Rossi Amy P. , Gatti Daniel M. , Beamer Gillian , Sassetti Christopher M. , Elkins Karen L.
Published: March 2024

Mycobacterium tuberculosis (M.tb.) infection leads to over 1.5 million deaths annually, despite widespread vaccination with BCG at birth. Causes for the ongoing tuberculosis endemic are complex and include the failure of BCG to protect many against progressive pulmonary disease. Host genetics is one of the known factors implicated in susceptibility to primary tuberculosis, but less is known about the role that host genetics plays in controlling host responses to vaccination against M.tb. Here, we addressed this gap by utilizing Diversity Outbred (DO) mice as a small animal model to query genetic drivers of vaccine-induced protection against M.tb. DO mice are a highly genetically and phenotypically diverse outbred population that is well suited for fine genetic mapping. Similar to outcomes in people, our previous studies demonstrated that DO mice have a wide range of disease outcomes following BCG vaccination and M.tb. challenge. In the current study, we used a large population of BCG-vaccinated/M.tb.-challenged mice to perform quantitative trait loci mapping of complex infection traits; these included lung and spleen M.tb. burdens, as well as lung cytokines measured at necropsy. We found sixteen chromosomal loci associated with complex infection traits and cytokine production. QTL associated with bacterial burdens included a region encoding major histocompatibility antigens that are known to affect susceptibility to tuberculosis, supporting validity of the approach. Most of the other QTL represent novel associations with immune responses to M.tb. and novel pathways of cytokine regulation. Most importantly, we discovered that protection induced by BCG is a multigenic trait, in which genetic loci harboring functionally-distinct candidate genes influence different aspects of immune responses that are crucial collectively for successful protection. These data provide exciting new avenues to explore and exploit in developing new vaccines against M.tb.
Mediatization of corruption cases in Indonesia through comics journalism in Tempo magazine
Authors: Nugroho Catur , Fitriawan Rana Akbari
Published: March 2024

Corruption in Indonesia is a never-ending and pervasive issue, as the numerous cases prove. Many public officials, businesspeople and private parties have been involved and found guilty in corruption trials. The verdict of the judges’ panel against the accused of corruption cases in Indonesia did not dampen the enthusiasm of several corruption perpetrators who were incarcerated at Sukamiskin Prison in Bandung. This research aims to examine the construction of news in the comics journalism format in Tempo magazine, specifically focusing on how corrupt behaviour in the form of bribery committed by criminals in Sukamiskin Prison is portrayed. This analysis is motivated by the mediatization theory, which is further supported by the mediatization of journalism. This research uses a semiotic-material approach that analyses Tempo comics journalism texts with a focus on elements of symbolism, timing and frames. The research found that Tempo mediated the news about bribery by grafters in Sukamiskin Prison through elements of comics based on journalistic practices. The mediation effort consists of two distinct stages: firstly, journalists on the field engage in the work and, secondly, the composer of the comic constructs the mediation.
Editorial
Authors: Brezavšček Pia
Published: March 2024

Sometimes it is not enough to declaratively address the right topics if our ways of doing it are not supporting our efforts. This issue strives to present certain methodological, strategical, and tactical tools that can help us answer “how?”. How to act if we wish to have an inclusive, caring, empathetic, fair, non-hierarchical, forgiving, brave, and [insert a value] art or society? Certain positions, especially those whose gender or sexuality made them personally feel the injustices and the dissatisfaction with the prevalent ways of doing things, have a greater sensibility for those topics and, out of quite existential reasons, often also more imagination for inventing new ways of solving problems and creating the conditions for cooperation and co-existence.
Anal Bodies / Anal Politics
Authors: Dimitrov Slavcho
Published: March 2024

The text explores what I call the ‘anal body and politics’, that is the potentiality and the political implications of the body when mapped and morphologically rearticulated from another standpoint than the hegemonic ones of the Phallic body, the frontal body, the heteronormative body, the reproductive body, the (liberal) individual body and so on. Instead, the anal body proposes something more vital to the body itself, without reinforcing a hetero-racialcapitalistic logic of reproduction, self-possessiveness, verticality, and property/propriety. The body that could be, and continues, as always already, being a multitudinous body of differences, transversally configured in provisional, yet unstable, unity; the body which brings the politics of co-existing and transindividuality. I develop my argument by reparative reading, from behind, of feminist theories of sexual difference, followed by a critical excavation of the politics of refusal specific to the anti-social thesis in queer theory, and ‘rehabilitation’ of the forgotten liberationist theory of Guy Hocquenghem.
Tell me your truth
Authors: Alfirević Dragana
Published: March 2024

Reflecting upon Jasna Žmak’s performance this is my truth, tell me yours, Dragana Alfirević points to the questions that the performance raises about the limits and possibilities of art and about the unwillingness of our society to talk about things and to deal with these questions in a sensitive and responsible way. The opposite of toxic patriarchy, as the author puts it in the conclusion, is not matriarchy, but vulnerability.
Reframing, embodying and in-betweening: A conversation about experiences of doing practice-based research and research-creation
Authors: Woods Justine , Granados Francisco-Fernando , Gauntlett David
Published: March 2024

This article presents a conversation between one professor and two students in the Ph.D. programme at The Creative School, Toronto Metropolitan University, the first in Canada to offer a dedicated practice-based Ph.D. opportunity to candidates from all creative disciplines. The discussion covers our own experiences of practice-based research and research-creation and describes how this can be an extraordinarily powerful way to explore issues of identity, community, exclusion and inclusion, creativity and human existence. We each share our individual pathways to practice-based research through the lens of garment-making, performance and music making, and how we have each found a place in the university to explore ideas and research through these mediums. We consider our experiences of making, doing and thinking about practice, and what this means as an impactful and transformational source of knowledge.
The Red Shoes, M. Powell and E. Pressburger (dirs) (2023), 75th Anniversary Release, UK: Archers BFI Southbank, London, 29 November 2023
Authors: Sporton Gregory
Published: March 2024

Review of: The Red Shoes, M. Powell and E. Pressburger (dirs) (2023), 75th Anniversary Release, UK: Archers BFI Southbank, London, 29 November 2023

 

The Red Shoes: Behind the Mirror, Exhibition at the BFI Southbank, 10 November 2023–7 January 2024