bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of grand multiparity on adverse maternal outcomes: A prospective cohort study
Authors: Dasa Tamirat Tesfaye , Okunlola Michael A. , Dessie Yadeta
Published: October 2022

Background

Grand multiparity remains a risk factor for a wide range of obstetric complications, especially in developing countries. Grand multiparity has been shown to increase the risks of medical and obstetric complications during pregnancies. However, in a research setting, the risk factors associated with adverse maternal outcomes have yet to be adequately investigated among grand multiparity. Furthermore, there is limited information that examines the effect of grand multiparity on pregnancy outcomes in Ethiopia through prospective follow-up design.

Objective

This study aimed to investigate the effect of grand multiparity on pregnancy outcomes in selected public hospitals in the Sidama Region State of Ethiopia.

Methods

A prospective cohort study design was employed on 837 pregnant women who were admitted for delivery in selected public hospitals from January 1 to August 31, 2021. The study subjects were recruited during admission for labor and delivery. Every woman who was admitted to labor wards was screened for eligibility. The exposed group in this cohort was grand multiparity, and the non-exposed group was multiparity. Data collection was started from the first contact after admission and follow-up to discharge for adverse maternal outcomes. The risk factors for adverse maternal outcomes in grand multiparity were investigated using multivariable Poisson regression analysis. The risk factor was reported as an adjusted risk ratio (ARR) with a 95% confidence interval (CI). When theP-value was <0.05, statistical significance was declared.

Results

The cohort's overall cumulative incidence of adverse maternal outcomes were 39.9% (95%CI: 36.6, 43.4%). Among exposed groups, the incidence of adverse maternal outcomes were 47.1% (95%CI: 41.0–53.2) and 36.3% (95% CI: 32.3–40.6) the multiparity. When compared to multiparous women, grand multiparity was associated with a greater risk of postpartum hemorrhage (ARR = 2.1; 95%CI:1.6–2.7) and malpresentation (ARR = 1.3; 95% CI: 1.01–1.7).

Conclusions

Pregnant women with grand multiparity have a higher incidence of adverse maternal outcomes. Grand multiparity increased the risk of adverse maternal outcomes such as postpartum bleeding and malpresentation. In low-resource settings, we recommend that community health education, the provision of accessible and effective contraceptive services, and increased awareness of the adverse maternal outcome among grand multiparity during pregnancy on obstetric performance should be prioritized. Also, trained health providers can effectively decrease the risk factor with good antenatal care and delivery.
Analyses of reported severe adverse events after immunization with SARS-CoV-2 vaccines in the United States: One year on
Authors: Mangat Halinder S. , Musah Anwar , Luedtke Susanne , Syed Akheel A. , Maramattom Boby V. , Maruthanal Joel , Bosman Arnold , Kostkova Patty
Published: October 2022

Objective

To analyze rates of reported severe adverse events after immunization (sAEFI) attributed to SARS-CoV-2 vaccines in the United States (US) using safety surveillance data.

Methods

Observational study of sAEFI reported to the vaccine adverse events reporting system (VAERS) between December 13, 2020, to December 13, 2021, and attributed to SARS-CoV-2 vaccination programs across all US states and territories. All sAEFI in conjunction with mRNA (BNT-162b2 or mRNA-1273) or adenovector (Ad26.COV2.S) vaccines were included. The 28-day crude cumulative rates for reported emergency department (ED) visits and sAEFI viz. hospitalizations, life-threatening events and deaths following SARS-CoV-2 vaccination were calculated. Incidence rate ratios (IRRs) of reported sAEFI were compared between mRNA and adenovector vaccines using generalized Poisson regression models.

Results

During the study period, 485 million SARS-CoV-2 vaccines doses were administered nationwide, and 88,626 sAEFI reported in VAERS. The 28-day crude cumulative reporting rates per 100,000 doses were 14.97 (95% confidence interval, 14.86–18.38) for ED visits, 5.32 (5.26–5.39) for hospitalizations, 1.72 (1.68–1.76) for life-threatening events, and 1.08 (1.05–1.11) for deaths. Females had two-fold rates for any reported AEFI compared to males, but lower adjusted IRRs for sAEFI. Cumulative rates per dose for reported sAEFI attributed to adenovector vaccine were 2–3-fold higher, and adjusted IRRs 1.5-fold higher than mRNA vaccines.

Conclusions

Overall cumulative rates for reported sAEFI following SARS-CoV-2 vaccination in the US over 1 year were very low; single-dose adenovector vaccine had 1.5-fold higher adjusted rates for reported sAEFI, which may however equate with multiple-doses mRNA vaccine regimens. These data indicate absence of high risks of sAEFI following SARS-CoV-2 vaccines and support safety equipoise between mRNA and adenovector vaccines. Public health messaging of these data is critical to overcome heuristic biases. Furthermore, these data may support ongoing adenovector vaccine use, especially in low- and middle-income countries due to affordability, logistical and cold chain challenges.
The impact of physical activity on the quality of life of a sample of Italian people with physical disability
Authors: Biagini Alessia , Bastiani Luca , Sebastiani Laura
Published: October 2022

Physical activity promotes psychophysical health; however, to date, only few studies have investigated the association between regular physical activity and the quality-of-life perception and satisfaction in disabled persons. Our aim was to compare the quality and satisfaction for life, self-efficacy, and personal wellbeing of two samples of Italian people with physical disability (amputation, spinal cord dysfunction, neurological disability): one group with people who practiced regular physical activity (active group, n = 33) and the other group consisting of sedentary individuals (inactive group, n = 26). We compared the mean scores of the groups in the World Health Organization Quality of Life Questionnaire (WHOQoL-BRIEF), the Personal Wellbeing Index, and the Satisfaction With Life and General Self-Efficacy scales. We also compared the impact of rehabilitation, sport, family support, income, job/school, and raising a family on social life and life quality by means of separate MANOVAs. Results of the WHOQoL showed better quality of life in the active than in the inactive group (overall QOL, 4.09 ± 0.7 vs. 3.50 ± 0.9; psychological domain, 72.09 ± 12.7 vs. 62 ± 21.6; social relationships domain, 76.54 ± 16.4 vs. 59.52 ± 24.2). No difference was found for satisfaction with health and life, personal wellbeing, and self-efficacy. The impact of sport on social life and quality of life was greater in active than in inactive individuals. Findings suggest positive effects of physical activity on the perception of quality of life in disabled people. However, they do not allow disentangling whether physical activity is practiced by patients with good quality of life or whether physical activity is responsible for better quality of life.
A novel scoring system to predict the residual back pain after percutaneous kyphoplasty for osteoporotic vertebral compression fracture
Authors: Yang Dongjun , Liu Xin , Zhou Yang , Xu Yong , Huang Qiangkai
Published: October 2022

Objective

To establish a scoring system to predict the residual back pain after percutaneous kyphoplasty (PKP) for osteoporotic vertebral compression fracture (OVCF).

Materials and methods

We retrospectively reviewed the clinical records of 98 patients who were diagnosed of single-vertebral OVCF and underwent PKP surgery in our department from January 2015 to December 2017. The following clinical characteristics including age, gender, disease course, fracture location, fracture type, segmental kyphosis, and bone cement volume were all recorded, and the effects of these factors on postoperative pain (at 1-month and 6-month postoperative) were also analyzed respectively. Based on 6-month postoperative VAS score, the included patients were divided into two groups, namely the residual back pain group (19 patients) and the non-residual back pain group (79 patients). The independent risk factors of residual back pain after PKP were screened and the scoring system was established by the multivariate logistic regression analysis. The performance of this scoring system was also prospectively validated using the clinical data of 45 patients with single-vertebral OVCF from January 2018 to December 2019.

Results

The scoring system was consist of five clinical characteristics which were confirmed as significant predictors of residual back pain after PKP, namely, age ≥60 years (P = 0.021), fracture location = thoracic or lumbar (P = 0.002), fracture type = OF4 type (P = 0.018), segmental kyphosis ≥20° (P = 0.014), and bone cement volume <5 ml (P = 0.001). Patients in the residual back pain group showed a significant higher score than the non-residual back pain group (6.84 ± 1.71 vs. 2.66 ± 1.97, t = 8.499, P < 0.001), and the optimal cut-off value for the scoring system was 5 points. The sensitivity and specificity of the scoring system for predicting residual back pain after PKP were 84.21% and 87.34%, respectively, in derivation set and 78.57% and 83.87% in validation set.

Conclusion

This novel scoring system showed satisfactory diagnostic efficacy in predicting residual back pain after PKP for single-vertebral OVCF. Patients with the score of 5–9 had a high risk of postoperative residual back pain, while the patients with score of 0–4 was low.
Linear Parameter-Varying Model Predictive Control for Hydraulic Wind Turbine
Authors: Han Bin , Gao Hongyan
Published: October 2022

Wind speed uncertainty and measurement noise affect the control effect in hydraulic wind turbine systems. This paper proposes a model predictive control (MPC) method with a dynamic Kalman filter (KF) based on a linear parameter-varying (LPV) model to address this problem. First of all, the LPV model for a nonlinear system of a hydraulic wind turbine is established using function substitution. Then, a LPV-based KF is introduced into the MPC to provide more precise estimated results and improve the anti-interference ability of the system. According to the current condition of the hydraulic wind turbine, the method updates the Kalman state estimator at each sampling instant and computes the optimal control input by solving a quadratic programming (QP) optimization problem. The performance and the efficiency of the proposed method is validated in simulation and compared with other methods.
Evaluation of Environmental Lactococcus lactis Strains Reveals Their Potential for Biotransformation of Lignocellulosic Feedstocks
Authors: Román Naranjo Desirée , Callanan Michael , Thierry Anne , McAuliffe Olivia
Published: October 2022

In this study, the potential for a collection of wild-type L. lactis strains to metabolize the breakdown products of lignocellulose was investigated. The strains, isolated from a variety of environmental sources including grass and vegetables, were analyzed for their ability to ferment pentose sugars and their cellulolytic ability. In total, 21 environment-derived L. lactis strains were evaluated. Eleven of the 21 L. lactis isolates were found to have the potential to ferment pentose sugars commonly produced by lignocellulose breakdown. A 3,5-dinitrosalicylic acid (DNS)-based cellulase assay was performed, and 10 of the 21 L. lactis isolates showed cellulolytic activity. Six strains were able to both metabolize pentose sugars and showed cellulolytic activity: these included green pea isolates DPC 6754, DPC 6755, DPC 6756, and DPC 6758, the grass isolate DPC 6760, and the mung bean sprouts isolate KF147. For the first time, certain wild-type non-engineered L. lactis were found to possess cellulolytic activity. Moreover, these two abilities do not appear to be correlated. These findings highlight that environment-derived L. lactis, a species with a history of safe use in food production, has the potential for second-generation bioconversion processes, and the potential to re-utilize plant biomass found in waste streams.
Analyzing the Electronics of Image Sensors and Their Functionality to Develop Low Light-Emitting Source Image
Authors: Chiranjeevi Rai , Indragandhi Vairavasundaram , Gunapriya Devarajan , Subramaniyaswamy Vairavasundaram , Jasiński Michał , Suresh Vishnu , Leonowicz Zbigniew
Published: October 2022

Bioluminescence imaging has been used to visualize the biological effects of human beings and is a promising technique in a recent modality. In this study, the digital image technique is used to improve quality and recover images. The optical fluence that emerges from the source is generated using a camera, and a low resgolution is observed. In this paper, the diurnal change of ultra-weak photon emission was successfully imaged with an improved, highly sensitive imaging system using a charge-coupled device (CCD) camera. The changes in energy metabolism might be linked with diurnal changes in photon emission, and when observed, the body emits extremely weak light spontaneously without external photoexcitation. Therefore, to obtain accurate information, a combined Barn Door Star Tracker approach has been proposed to improve the accuracy of the method and has been implemented to test on celestial bodies. The ability to temporally assess the location of star movement can be monitored accurately with bioluminescence imaging.
Expression and Purification of Recombinant SARS-CoV-2 Accessory Protein ORF7a and Functional Analysis of Its Role in Up-Regulating Cytokine Production
Authors: Chen Dan , Zheng Zhenhua , Han Zhenggang
Published: October 2022

The severity of coronavirus disease 2019 is closely linked to dysregulated immune responses. The search for viral proteins associated with immune regulation in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical to reveal the pathogenicity of the virus. In this study, accessory proteins ORF7a (referred to as ORF7a-1 and ORF7a-2, respectively) from two SARS-related coronaviruses, severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2, were produced through the denaturing and refolding of inclusion body proteins. The recombinant protein was incubated with alveolar epithelial cells, and the transcription and expression levels of major cytokines were determined by reverse transcription-quantitative PCR and enzyme-linked immunosorbent assay. SARS-related coronavirus ORF7a can up-regulate the transcription and expression of interleukin-6, C-C motif chemokine ligand 8, interferon α, and interferon β. The results also indicated that the two highly conserved ORF7a had certain differences in promoting the transcription and expression of cytokines. The study showed that ORF7a is a virus-encoded immune regulator by alveolar epithelial cells that plays an important role in the pathogenicity of SARS-related coronaviruses.
Scatter Irradiation of Rat Brain Triggers Sex- and Brain Region-Specific Changes in the Expression of Non-Coding RNA Fragments
Authors: Fiselier Anna , Byeon Boseon , Ilnytskyy Yaroslav , Kovalchuk Olga , Kovalchuk Igor
Published: October 2022

Non-coding RNA fragments (ncRFs) are small RNA fragments processed from non-coding RNAs (ncRNAs). ncRFs have various functions and are commonly tissue-specific, and their processing is altered by exposure to stress. Information about ncRFs in the brain is scarce. Recently, we reported the brain region-specific and sex-specific expression of ncRNAs and their processing into ncRFs. Here, we analyzed the expression of ncRFs in the frontal cortex (FC), hippocampus (HIP), and cerebellum (CER) of male and female rats exposed to scatter radiation. We found multiple brain region- and sex-specific changes in response to scatter radiation. Specifically, we observed decreased miRNA expression and the increased expression of ra-ncRNA reads in HIP and CER, as well as an increased number of mtR-NA-associated reads in HIP. We also observed the appearance of sense-intronic ncRNAs—in females, in HIP and FC, and in males, in CER. In this work, we also show that tRNA-GlyGCC and tRNA-GlyCCC are most frequently processed to tRFs, in CER in females, as compared to males. An analysis of the targeted pathways revealed that tRFs and snoRFs in scatter radiation samples mapped to genes in several pathways associated with various neuronal functions. While in HIP and CER these pathways were underrepresented, in FC, they were overrepresented. Such changes may play an important role in pathologies that develop in response to scatter radiation, the effect known as “radio-brain”, and may in part explain the sex-specific differences observed in animals and humans exposed to radiation and scatter radiation.
A Novel Implementation of Mönch’s Fixed Point Theorem to a System of Nonlinear Hadamard Fractional Differential Equations
Authors: Al Elaiw Abeer , Awadalla Muath , Manigandan Murugesan , Abuasbeh Kinda
Published: October 2022

In this article, we employed Mönch’s fixed point theorem to investigate the existence of solutions for a system of nonlinear Hadamard fractional differential equations and nonlocal non-conserved boundary conditions in terms of Hadamard integral. Followed by a study of the stability of this solution using the Ulam-Hyres technique. This study concludes with an applied numerical example that helps in understanding the theoretical results obtained.
Do Health Information Sources Influence Health Literacy among Older Adults: A Cross-Sectional Study in the Urban Areas of Western China
Authors: Li Chengbo , Liu Mengyao , Zhou Jin , Zhang Mei , Liu Huanchang , Wu Yuting , Li Hui , Leeson George W. , Deng Tingting
Published: October 2022

Background: Previous studies have found that the dissemination pattern and delivery mechanism of information can provide crucial resources and empowerment for individuals to the promotion of health literacy. The present study investigates how health information sources are associated with health literacy among older adults in west China, and tries to explain the mechanisms underlying the link between health information sources and health literacy in the Chinese context. Methods: The cross-sectional study employed a representative sample of 812 urban citizens aged 60 and older in 2017 in Western China. Results: We found that health information sources including healthcare practitioners (B = 4.577, p < 0.001), neighbors (B = 2.545, p < 0.05), newspapers (B = 4.280, p < 0.001), and television (B = 4.638, p < 0.001) were positively associated with health literacy. Additionally, age (B = −1.781, p < 0.001) was negatively associated with health literacy, and the socio-economic status factors including minority (B = −10.005, p < 0.001), financial strain status of perceived very difficult (B = −10.537, p < 0.001), primary school (B = 11.461, p < 0.001), junior high school (B = 18.016, p < 0.001), polytechnic school or senior high school (B = 21.905, p < 0.001), college and above (B = 23.433, p < 0.001) were significantly linked to health literacy, and suffering from chronic diseases (B = 3.430, p < 0.01) was also positively related to health literacy. Conclusions: Health information sources including healthcare practitioners, neighbors, newspapers, and television have a strong influence on health literacy, which implies that the four main types of sources are the important patterns of health information dissemination in the reinforcement of health literacy. In addition, the present findings also indicate age, minority and disease differences in health literacy and confirm the influence of enabling factors including educational attainment and financial strain on health literacy. Based on these findings and their implications, specific evidence is presented for the reinforcement of health literacy in interpersonal and mass communication, and in the educational and financial settings in the Chinese context. The present results also suggest that the age-specific, minority-specific and disease-specific measures should be taken to promote health literacy among older adults.
Evaluation of an Intervention to Promote Self-Management Regarding Cardiovascular Disease: The Social Engagement Framework for Addressing the Chronic-Disease-Challenge (SEFAC)
Authors: Korenhof Sophie A. , Rouwet Ellen V. , Elstgeest Liset E. M. , Tan Siok Swan , Macchione Stefania , Vasiljev Vanja , Rukavina Tomislav , Alhambra-Borrás Tamara , Fierloos Irene N. , Raat Hein
Published: October 2022

Background: Cardiovascular diseases (CVD) are predominantly lifestyle related. Mental health issues also influence CVD progression and quality of life. Self-management of lifestyle behaviors and mental well-being may play a significant role in reducing the CVD burden. Previous studies have shown that mindfulness practices are associated with psychological well-being, but their effects on CVD self-management are mainly unknown. Methods: The study had a before–after design and included adults over 50 years with CVD and/or one or more risk factors from three European countries. Follow-up was six months. The intervention was a 7-week mindfulness-based intervention (MBI) in a group setting focusing on chronic disease self-management. Outcomes were measured with validated self-report questionnaires at baseline and follow-up: self-efficacy, physical activity, nutrition, smoking, alcohol use, sleep and fatigue, social support, stress, depression, medication adherence, and self-rated health. Results: Among 352 participants, 324 (92%) attended ≥4 of the 7 group sessions and completed follow-up. During follow-up, self-efficacy, stress, social support, depressive symptoms, and self-rated health significantly improved. No significant changes were detected for other outcomes. Conclusions: A 7-week MBI focusing on chronic disease self-management was conducive to improved self-efficacy, emotional well-being, social support, and self-rated overall health during six months. These findings support the use of MBIs for improving self-management in cardiovascular care. ISRCTN registry-number ISRCTN11248135.
Diverse Sphingolipid Species Harbor Different Effects on Ire1 Clustering
Authors: Bieniawski Mark A. , Stevens Kofi L. P. , Witham Christopher M. , Steuart Robert F. L. , Bankaitis Vytas A. , Mousley Carl J.
Published: October 2022

Endoplasmic reticulum (ER) function is dedicated to multiple essential processes in eukaryotes, including the processing of secretory proteins and the biogenesis of most membrane lipids. These roles implicate a heavy burden to the organelle, and it is thus prone to fluctuations in the homeostasis of molecules which govern these processes. The unfolded protein response (UPR) is a general ER stress response tasked with maintaining the ER for optimal function, mediated by the master activator Ire1. Ire1 is an ER transmembrane protein that initiates the UPR, forming characteristic oligomers in response to irregularities in luminal protein folding and in the membrane lipid environment. The role of lipids in regulating the UPR remains relatively obscure; however, recent research has revealed a potent role for sphingolipids in its activity. Here, we identify a major role for the oxysterol-binding protein Kes1, whose activity is of consequence to the sphingolipid profile in cells resulting in an inhibition of UPR activity. Using an mCherry-tagged derivative of Ire1, we observe that this occurs due to inhibition of Ire1 to form oligomers. Furthermore, we identify that a sphingolipid presence is required for Ire1 activity, and that specific sphingolipid profiles are of major consequence to Ire1 function. In addition, we highlight cases where Ire1 oligomerization is absent despite an active UPR, revealing a potential mechanism for UPR induction where Ire1 oligomerization is not necessary. This work provides a basis for the role of sphingolipids in controlling the UPR, where their metabolism harbors a crucial role in regulating its onset.
Experimental Study of the Impact of Trace Amounts of Acetylene and Methylacetylene on the Synthesis, Mechanical and Thermal Properties of Polypropylene
Authors: Hernández-Fernández Joaquín , Vivas-Reyes Ricardo , Toloza Carlos A. T.
Published: October 2022

During the production of polymer-grade propylene, different processes are used to purify this compound and ensure that it is of the highest quality. However, some impurities such as acetylene and methyl acetylene are difficult to remove, and some of these impurities may be present in the propylene used to obtain polypropylene, which may have repercussions on the process. This study evaluates the impact of these acetylene and methyl acetylene impurities on the productivity of the polypropylene synthesis process and on the mechanical and thermal properties of the material obtained through the synthesis of eight samples with different concentrations of acetylene and eight samples with different concentrations of acetylene. We discovered that for the first concentrations of both acetylene (2 and 3 ppm) and methyl acetylene (0.03 and 0.1), the MFI, thermal recording, and mechanical properties of the resin were unaffected by the variation of the fluidity index, thermal degradation by TGA, and mechanical properties such as resistance to tension, bending, and impact. However, when the concentration exceeded 14 ppm for methyl acetylene and 12 ppm for acetylene, the resistance of this resin began to decrease linearly. Regarding production, this was affected by the first traces of acetylene and methyl acetylene progressively decreasing.
Role of Omega-3 Fatty Acids as Non-Photic Zeitgebers and Circadian Clock Synchronizers
Authors: Checa-Ros Ana , D’Marco Luis
Published: October 2022

Omega-3 fatty acids (ω-3 FAs) are well-known for their actions on immune/inflammatory and neurological pathways, functions that are also under circadian clock regulation. The daily photoperiod represents the primary circadian synchronizer (‘zeitgeber’), although diverse studies have pointed towards an influence of dietary FAs on the biological clock. A comprehensive literature review was conducted following predefined selection criteria with the aim of updating the evidence on the molecular mechanisms behind circadian rhythm regulation by ω-3 FAs. We collected preclinical and clinical studies, systematic reviews, and metanalyses focused on the effect of ω-3 FAs on circadian rhythms. Twenty animal (conducted on rodents and piglets) and human trials and one observational study providing evidence on the regulation of neurological, inflammatory/immune, metabolic, reproductive, cardiovascular, and biochemical processes by ω-3 FAs via clock genes were discussed. The evidence suggests that ω-3 FAs may serve as non-photic zeitgebers and prove therapeutically beneficial for circadian disruption-related pathologies. Future work should focus on the role of clock genes as a target for the therapeutic use of ω-3 FAs in inflammatory and neurological disorders, as well as on the bidirectional association between the molecular clock and ω-3 FAs.
Role of Endothelial STAT3 in Cerebrovascular Function and Protection from Ischemic Brain Injury
Authors: Davis Catherine M. , Lyon-Scott Kristin , Varlamov Elena V. , Zhang Wenri H. , Alkayed Nabil J.
Published: October 2022

STAT3 plays a protective role against ischemic brain injury; however, it is not clear which brain cell type mediates this effect, and by which mechanism. We tested the hypothesis that endothelial STAT3 contributes to protection from cerebral ischemia, by preserving cerebrovascular endothelial function and blood–brain barrier (BBB) integrity. The objective of this study was to determine the role of STAT3 in cerebrovascular endothelial cell (EC) survival and function, and its role in tissue outcome after cerebral ischemia. We found that in primary mouse brain microvascular ECs, STAT3 was constitutively active, and its phosphorylation was reduced by oxygen-glucose deprivation (OGD), recovering after re-oxygenation. STAT3 inhibition, using two mechanistically different pharmacological inhibitors, increased EC injury after OGD. The sub-lethal inhibition of STAT3 caused endothelial dysfunction, demonstrated by reduced nitric oxide release in response to acetylcholine and reduced barrier function of the endothelial monolayer. Finally, mice with reduced endothelial STAT3 (Tie2-Cre; STAT3flox/wt) sustained larger brain infarcts after middle cerebral artery occlusion (MCAO) compared to wild-type (WT) littermates. We conclude that STAT3 is vital to maintaining cerebrovascular integrity, playing a role in EC survival and function, and protection against cerebral ischemia. Endothelial STAT3 may serve as a potential target in preventing endothelial dysfunction after stroke.
Enhanced Succinate Oxidation with Mitochondrial Complex II Reactive Oxygen Species Generation in Human Prostate Cancer
Authors: Zhang Aijun , Gupte Anisha A. , Chatterjee Somik , Li Shumin , Ayala Alberto G. , Miles Brian J. , Hamilton Dale J.
Published: October 2022

The transformation of prostatic epithelial cells to prostate cancer (PCa) has been characterized as a transition from citrate secretion to citrate oxidation, from which one would anticipate enhanced mitochondrial complex I (CI) respiratory flux. Molecular mechanisms for this transformation are attributed to declining mitochondrial zinc concentrations. The unique metabolic properties of PCa cells have become a hot research area. Several publications have provided indirect evidence based on investigations using pre-clinical models, established cell lines, and fixed or frozen tissue bank samples. However, confirmatory respiratory analysis on fresh human tissue has been hampered by multiple difficulties. Thus, few mitochondrial respiratory assessments of freshly procured human PCa tissue have been published on this question. Our objective is to document relative mitochondrial CI and complex II (CII) convergent electron flow to the Q-junction and to identify electron transport system (ETS) alterations in fresh PCa tissue. The results document a CII succinate: quinone oxidoreductase (SQR) dominant succinate oxidative flux model in the fresh non-malignant prostate tissue, which is enhanced in malignant tissue. CI NADH: ubiquinone oxidoreductase activity is impaired rather than predominant in high-grade malignant fresh prostate tissue. Given these novel findings, succinate and CII are promising targets for treating and preventing PCa.
Single-Cell Transcriptomic Profiling in Inherited Retinal Degeneration Reveals Distinct Metabolic Pathways in Rod and Cone Photoreceptors
Authors: Chen Yiyi , Dong Yujie , Yan Jie , Wang Lan , Yu Shirley , Jiao Kangwei , Paquet-Durand François
Published: October 2022

The cellular mechanisms underlying hereditary photoreceptor degeneration are still poorly understood. The aim of this study was to systematically map the transcriptional changes that occur in the degenerating mouse retina at the single cell level. To this end, we employed single-cell RNA-sequencing (scRNA-seq) and retinal degeneration-1 (rd1) mice to profile the impact of the disease mutation on the diverse retinal cell types during early post-natal development. The transcriptome data allowed to annotate 43,979 individual cells grouped into 20 distinct clusters. We further characterized cluster-specific metabolic and biological changes in individual cell types. Our results highlight Ca2+-signaling as relevant to hereditary photoreceptor degeneration. Although metabolic reprogramming in retina, known as the ‘Warburg effect’, has been documented, further metabolic changes were noticed in rd1 mice. Such metabolic changes in rd1 mutation was likely regulated through mitogen-activated protein kinase (MAPK) pathway. By combining single-cell transcriptomes and immunofluorescence staining, our study revealed cell type-specific changes in gene expression, as well as interplay between Ca2+-induced cell death and metabolic pathways.
Hyaluronic Acid Scaffolds for Loco-Regional Therapy in Nervous System Related Disorders
Authors: Djoudi Amel , Molina-Peña Rodolfo , Ferreira Natalia , Ottonelli Ilaria , Tosi Giovanni , Garcion Emmanuel , Boury Frank
Published: October 2022

Hyaluronic acid (HA) is a Glycosaminoglycan made of disaccharide units containing N-acetyl-D-glucosamine and glucuronic acid. Its molecular mass can reach 10 MDa and its physiological properties depend on its polymeric property, polyelectrolyte feature and viscous nature. HA is a ubiquitous compound found in almost all biological tissues and fluids. So far, HA grades are produced by biotechnology processes, while in the human organism it is a major component of the extracellular matrix (ECM) in brain tissue, synovial fluid, vitreous humor, cartilage and skin. Indeed, HA is capable of forming hydrogels, polymer crosslinked networks that are very hygroscopic. Based on these considerations, we propose an overview of HA-based scaffolds developed for brain cancer treatment, central and peripheral nervous systems, discuss their relevance and identify the most successful developed systems.
RLPredictiOme, a Machine Learning-Derived Method for High-Throughput Prediction of Plant Receptor-like Proteins, Reveals Novel Classes of Transmembrane Receptors
Authors: Silva Jose Cleydson F. , Ferreira Marco Aurélio , Carvalho Thales F. M. , Silva Fabyano F. , de A. Silveira Sabrina , Brommonschenkel Sergio H. , Fontes Elizabeth P. B.
Published: October 2022

Cell surface receptors play essential roles in perceiving and processing external and internal signals at the cell surface of plants and animals. The receptor-like protein kinases (RLK) and receptor-like proteins (RLPs), two major classes of proteins with membrane receptor configuration, play a crucial role in plant development and disease defense. Although RLPs and RLKs share a similar single-pass transmembrane configuration, RLPs harbor short divergent C-terminal regions instead of the conserved kinase domain of RLKs. This RLP receptor structural design precludes sequence comparison algorithms from being used for high-throughput predictions of the RLP family in plant genomes, as has been extensively performed for RLK superfamily predictions. Here, we developed the RLPredictiOme, implemented with machine learning models in combination with Bayesian inference, capable of predicting RLP subfamilies in plant genomes. The ML models were simultaneously trained using six types of features, along with three stages to distinguish RLPs from non-RLPs (NRLPs), RLPs from RLKs, and classify new subfamilies of RLPs in plants. The ML models achieved high accuracy, precision, sensitivity, and specificity for predicting RLPs with relatively high probability ranging from 0.79 to 0.99. The prediction of the method was assessed with three datasets, two of which contained leucine-rich repeats (LRR)-RLPs from Arabidopsis and rice, and the last one consisted of the complete set of previously described Arabidopsis RLPs. In these validation tests, more than 90% of known RLPs were correctly predicted via RLPredictiOme. In addition to predicting previously characterized RLPs, RLPredictiOme uncovered new RLP subfamilies in the Arabidopsis genome. These include probable lipid transfer (PLT)-RLP, plastocyanin-like-RLP, ring finger-RLP, glycosyl-hydrolase-RLP, and glycerophosphoryldiester phosphodiesterase (GDPD, GDPDL)-RLP subfamilies, yet to be characterized. Compared to the only Arabidopsis GDPDL-RLK, molecular evolution studies confirmed that the ectodomain of GDPDL-RLPs might have undergone a purifying selection with a predominance of synonymous substitutions. Expression analyses revealed that predicted GDPGL-RLPs display a basal expression level and respond to developmental and biotic signals. The results of these biological assays indicate that these subfamily members have maintained functional domains during evolution and may play relevant roles in development and plant defense. Therefore, RLPredictiOme provides a framework for genome-wide surveys of the RLP superfamily as a foundation to rationalize functional studies of surface receptors and their relationships with different biological processes.
The Detection of Immunity against WT1 and SMAD4P130L of EpCAM+ Cancer Cells in Malignant Pleural Effusion
Authors: Koya Terutsugu , Niida Yo , Togi Misa , Yoshida Kenichi , Sakamoto Takuya , Ura Hiroki , Togi Sumihito , Kato Tomohisa , Yamada Sohsuke , Sugiyama Haruo , Koido Shigeo , Shimodaira Shigetaka
Published: October 2022

Malignant pleural effusion (MPE) provides a liquid tumor microenvironment model that includes cancer cells and immune cells. However, the characteristics of tumor antigen-specific CD8+ T cells have not been investigated in detail. Here, we analyzed MPE samples taken from a patient with pancreatic cancer who received a dendritic cell vaccine targeting Wilms’ Tumor 1 (WT1) antigen over the disease course (two points at MPE1st and 2nd, two months after MPE1st). Epithelial cell adhesion molecule (EpCAM)+ cancer cells (PD-L1− or T cell immunoglobulin mucin-3, TIM-3−), both PD-1 or TIM-3 positive CD8+ T cells, and CD14+CD68+CD163+TIM-3+ macrophages increased from the MPE1st to MPE2nd. The ratio of WT1-specific cytotoxic lymphocytes (WT1-CTLs) to MPE CD8+ T cells and IFN-γ secretion of WT1-CTLs were reduced with disease progression. Coincidentally, the fraction of central memory T (TCM) of WT1-CTLs was decreased. On the other hand, CD8+ T cells in response to SMAD4P130L, which is homogeneously expressed in EpCAM+ cancer cells, were detected using in vitro expansion with the HLA-A*11:01 restrictive SVCVNLYH neoantigen. Furthermore, the CD8+ T cell response to SMAD4P130L was diminished following remarkably decreased numbers of CD8+ TCM in MPE samples. In conclusion, CD8+ T cells responding to WT1 or SMAD4P130L neoantigen expressed in EpCAM+ pancreatic cancer cells were detected in MPE. A tumor antigen-specific immune response would provide novel insight into the MPE microenvironment.
TcMYB8, a R3-MYB Transcription Factor, Positively Regulates Pyrethrin Biosynthesis in Tanacetum cinerariifolium
Authors: Zhou Li , Li Jiawen , Zeng Tuo , Xu Zhizhuo , Luo Jing , Zheng Riru , Wang Yuanyuan , Wang Caiyun
Published: October 2022

Pyrethrins are a mixture of terpenes, with insecticidal properties, that accumulate in the aboveground parts of the pyrethrum (Tanacetum cinerariifolium). Numerous studies have been published on the positive role of MYB transcription factors (TFs) in terpenoid biosynthesis; however, the role of MYB TFs in pyrethrin biosynthesis remains unknown. Here, we report the isolation and characterization of a T. cinerariifolium MYB gene encoding a R3-MYB protein, TcMYB8, containing a large number of hormone-responsive elements in its promoter. The expression of the TcMYB8 gene showed a downward trend during the development stage of flowers and leaves, and was induced by methyl jasmonate (MeJA), salicylic acid (SA), and abscisic acid (ABA). Transient overexpression of TcMYB8 enhanced the expression of key enzyme-encoding genes, TcCHS and TcGLIP, and increased the content of pyrethrins. By contrast, transient silencing of TcMYB8 decreased pyrethrin contents and downregulated TcCHS and TcGLIP expression. Further analysis indicated that TcMYB8 directly binds to cis-elements in proTcCHS and proTcGLIP to activate their expression, thus regulating pyrethrin biosynthesis. Together, these results highlight the potential application of TcMYB8 for improving the T. cinerariifolium germplasm, and provide insight into the pyrethrin biosynthesis regulation network.
Energy-Saving Load Control of Induction Electric Motors for Drives of Working Machines to Reduce Thermal Wear
Authors: Al-Quraan Tareq M. A. , Vovk Oleksandr , Halko Serhii , Kvitka Serhii , Suprun Olena , Miroshnyk Oleksandr , Nitsenko Vitalii , Zayed Nurul Mohammad , Islam K. M. Anwarul
Published: October 2022

The influence of reduced voltage on the service life of an induction motor is considered in this article. An algorithm for calculating the rate of thermal wear of induction motor insulation under reduced supply voltage depending on the load and the mechanical characteristics of the working machine has been developed. It determines the change in the rate of thermal wear under alternating external effects on the motor (supply voltage and load) and allows forecasting its service life under these conditions. The dependency graphs of the rate of insulation thermal wear on the motor load for various levels of supply voltage and various mechanical characteristics of working machines are provided in the work. It was determined that the rate of thermal wear of the induction motor insulation increases significantly when the voltage is reduced compared to its nominal value with nominal load on the motor. The authors propose to consider this fact for resource-saving control of the motor. The paper presents the results of experimental verification of the obtained rule for “Asynchronous Interelectro” (AI) series electric motors that confirm its accuracy. Based on the obtained correlation, the rule of voltage regulation in energy-saving operation mode has been derived. The proposed rule takes into account the thermal impact on the electric motor running in energy-saving mode and enables saving its resource, which, in turn, results in extending its service life. The research does not consider additional effects on the electric motor except the thermal one.
Process Optimization for the 3D Printing of PLA and HNT Composites with Arburg Plastic Freeforming
Authors: Engler Leonardo G. , Crespo Janaina S. , Gately Noel M. , Major Ian , Devine Declan M.
Published: October 2022

The industrial use of additive manufacturing continues to rapidly increase as new technology developments become available. The Arburg plastic freeforming (APF) process is designed to utilize standard polymeric granules in order to print parts with properties similar to those of molded parts. Despite the emerging industrial importance of APF, the current body of knowledge regarding this technology is still very limited, especially in the field of biodegradable polymer composites. To this end, poly(lactic acid) (PLA) was reinforced with halloysite nanotubes (HNTs) by hot melt extrusion. The PLA/HNT (0–10 wt%.) composites were analyzed in terms of their rheology, morphology, and thermal and mechanical properties. A study of the processing properties of these composites in the context of APF was performed to ensure the consistency of 3D-printed, high-quality components. The optimized machine settings were used to evaluate the tensile properties of specimens printed with different axis orientations (XY and XZ) and deposition angles (0 and 45°). Specimens printed with an XY orientation and deposition angle starting at 0° resulted in the highest mechanical properties. In this study, the use of PLA/HNT composites in an APF process was reported for the first time, and the current methodology achieved satisfactory results in terms of the 3D printing and evaluation of successful PLA/HNT composites to be used as feedstock in an APF process.
Implementation of Good Practices in Environmental Licensing Processes
Authors: de Godoi Emiliano Lobo , Mendes Thiago Augusto , Batalhão André C. S.
Published: October 2022

One of the main command and control mechanisms for the environment is the environmental licensing process. However, the isolated adoption of this mechanism has not satisfactorily ensured the environmental quality of natural resources. On the other hand, an increasing number of organizations are voluntarily adhering to good environmental practices. As a result, approximating the relationship between command and control instruments (state regulation) and good practices becomes increasingly important for improving the environmental performance of organizations. Within this context, the present work proposes to evaluate how good environmental practices can strengthen and advance current environmental licensing models adopted in South America. This research consists of an exploratory case study, conducted with a qualitative approach. Several countries in South America were evaluated because of the great natural resources that the region has, as well as the European Economic Community, due to their adoption of the so-called “best available techniques” of environmental management since 1996. The results of the study indicated that voluntary adherence to international environmental management standards has evolved in the analyzed countries and that environmental agencies in South American countries could establish legal bases to consolidate the inclusion of good environmental practices in environmental licensing processes. It was also observed that some benefits could be given to organizations that implement good environmental practices, such as granting of licenses with extended terms; debureaucratization (with time gain) of administrative procedures; exemption or reduction of fees; and facilitation of financing. This study could thus support debates for the effective advancement of the current environmental licensing model. However, this hypothesis must be evaluated and consolidated through further research, carried out individually for each country analyzed.
Study on the Effects of the Composite Addition of Al–5Ti–0.8C and La on the Microstructure and High-Temperature Mechanical Properties of ZL205A Alloy
Authors: Li Yu , Zhang Guowei , Niu Jingwei , Xu Hong
Published: October 2022

The effects of Al–5Ti–0.8C and the rare-earth element La on the microstructure and high-temperature mechanical properties of ZL205A alloy were investigated. We found that the grains of 0.1%La + 0.3%Al–5Ti–0.8C alloy were fine, the morphology of the as-cast Al2Cu phase was fragmented, and the precipitated phase was fine after T5 treatment. In particular, the high-temperature mechanical properties of 0.1%La + 0.3%Al–5Ti–0.8C alloy were significantly improved above 250 °C. The reason for the increase in high-temperature plasticity was attributed to the Al–La phase and the TiC particles, which refined the grains and reduced the tendency for intergranular fracture at high temperatures. More importantly, the high-temperature strengthening mechanism of the Al–5Ti–0.8C master alloy compounded with La was a result of the TiC introduced by the Al–5Ti–0.8C alloy, and the Al11La3 formed by the addition of La refined the grains in the matrix, promoted the precipitation of the needle-like θ’(Al2Cu) phase, reduced the size of the θ’(Al2Cu) phase, decreased the PFZ (Precipitation Free Zone), and increased the θ’(Al2Cu) phase number, hindering dislocation and grain boundary motion.