bluuebunny/crossref_metadata_2025 huggingface dataset:
Dispatcher instructions for bystander cardiopulmonary resuscitation and neurologically intact survival after bystander-witnessed out-of-hospital cardiac arrests: a nationwide, population-based observational study
Authors: Goto Yoshikazu , Funada Akira , Maeda Tetsuo , Goto Yumiko
Published: November 2021

Background

The International Liaison Committee on Resuscitation recommends that dispatchers provide instructions to perform compression-only cardiopulmonary resuscitation (CPR) to callers responding to adults with out-of-hospital cardiac arrest (OHCA). This study aimed to determine the optimal dispatcher-assisted CPR (DA-CPR) instructions for OHCA.

Methods

We analysed the records of 24,947 adult patients (aged ≥ 18 years) who received bystander DA-CPR after bystander-witnessed OHCA. Data were obtained from a prospectively recorded Japanese nationwide Utstein-style database for a 2-year period (2016–2017). Patients were divided into compression-only DA-CPR (n = 22,778) and conventional DA-CPR (with a compression-to-ventilation ratio of 30:2,n = 2169) groups. The primary outcome measure was 1-month neurological intact survival, defined as a cerebral performance category score of 1–2 (CPC 1–2).

Results

The 1-month CPC 1–2 rate was significantly higher in the conventional DA-CPR group than in the compression-only DA-CPR group (before propensity score (PS) matching, 7.5% [162/2169] versus 5.8% [1309/22778],p < 0.01; after PS matching, 7.5% (162/2169) versus 5.7% (123/2169),p < 0.05). Compared with compression-only DA-CPR, conventional DA-CPR was associated with increased odds of 1-month CPC 1–2 (before PS matching, adjusted odds ratio 1.39, 95% confidence interval [CI] 1.14–1.70,p < 0.01; after PS matching, adjusted odds ratio 1.34, 95% CI 1.00–1.79,p < 0.05).

Conclusion

Within the limitations of this retrospective observational study, conventional DA-CPR with a compression-to-ventilation ratio of 30:2 was preferable to compression-only DA-CPR as an optimal DA-CPR instruction for coaching callers to perform bystander CPR for adult patients with bystander-witnessed OHCAs.
Perceived study-induced influence on the control group in a randomized controlled trial evaluating a complex intervention to promote psychosocial well-being after stroke: a process evaluation
Authors: Mangset Margrete , Kitzmüller Gabriele , Evju Anne S. , Angel Sanne , Aadal Lena , Martinsen Randi , Bronken Berit Arnesveen , Kvigne Kari J. , Bragstad Line K. , Hjelle Ellen Gabrielsen , Sveen Unni , Kirkevold Marit
Published: November 2021

Background

A commonly applied control condition in trials evaluating complex interventions in rehabilitation research is “usual care.” The main challenge is to ensure that the control group receives genuine usual care as delivered in everyday clinical practice. The assessment interviews and dialogues with the data collectors may influence the control group participants’ reflections on their condition and adjustments. This represents a threat to the internal validity of the trial. Thus, the aim of this study was to explore the perceived study-induced influence of assessment interviews on the adjustment of the members of a control group in a randomized clinical trial. The aim of the trial was to test a dialogue-based psychosocial intervention aiming at promoting the psychosocial well-being and adjustment of stroke survivors.

Methods

Fifteen participants in the control group of a multicenter stroke rehabilitation trial participated in narrative semi-structured interviews. Ricoeur’s interpretation theory guided the analysis.

Results

The perceived study-induced influence of the assessment interviews on the adjustment process of members of the control group varied considerably. The results demonstrated that the assessment interviews facilitated some participants’ feelings of control and their ability to cope. Other participants’ statements indicate that they relied on their existing personal capacity to cope and adjust and that the assessment interviews did not make any difference either on their coping ability or on their process of adjustment.

Five themes were identified that described the perceived study-induced influence of the assessment interviews in the control group. The themes illustrated that the assessments served as a safety net, enhanced awareness and understanding, encouraged seeking support, allowed the opportunity to vent disappointment, or did not make any difference either way.

Conclusions

RCT assessment interviews may influence the adjustment process and represent a serious problem in measuring interventions over time in trials of complex interventions in rehabilitation research. To uphold rigor and stringency, the usual care control conditions should be thoroughly assessed and described. Informing participants only about the treatment they were allocated to receive might counteract the potential to dilute the difference between the two arms of the trial.

Trial registration

ClinicalTrials.gov NCT02338869. Registered on October 4, 2014
The place of S-ketamine in fibromyalgia treatment (ESKEFIB): study protocol for a prospective, single-center, double-blind, randomized, parallel-group, dose-escalation controlled trial
Authors: Javorcikova Zuzana , Dangoisse Michel , Nikis Stéphane , Lechat Jean-Paul , Gillain Aline , Fils Jean-François , Van der Linden Philippe
Published: November 2021

Background

Fibromyalgia is a chronic multidimensional pain disease with no curative treatment currently available. Its management relies on a multimodal approach involving pharmacologic and non-pharmacologic elements. Because a suggested factor in its etiology is a central sensitization phenomenon involving the N-methyl-D-aspartate receptor (NMDAR), NMDAR antagonists have been proposed as a treatment target. Ketamine and its levogyre form, S-ketamine, have been used to treat chronic pain for many years without consensus about their therapeutic efficiency. We aim to assess the efficacy of S-ketamine as a co-treatment for fibromyalgia.

Methods

This prospective, randomized, single-center, double-blind, parallel-group, dose-escalation trial will compare a co-treatment with S-ketamine (intervention) to a control treatment without S-ketamine (control). It will consist of two successive cohorts with 2:1 randomization ratio (S-ketamine at two different doses: control) with 105 participants in each cohort. The protocol follow-up time will be 12 weeks, including 3 visits for the treatment (week 0, week 2, and week 4) and 3 visits for follow-up (week 6, week 9, and week 12). Our primary outcome, pain relief and/or better patient function, will be assessed with the Brief Pain Inventory questionnaire. The statistical analysis will be performed on an intention-to-treat basis. If the primary outcome is reached at the end of follow-up in the first cohort with low-dose S-ketamine (0.2 mg/kg), the trial will end. If not, the trial will continue with the second cohort and high-dose S-ketamine (0.4 mg/kg).

Discussion

The challenge of our trial is the inclusion of a large number of participants in comparison to other trials involving ketamine or S-ketamine infusions for chronic pain management. The originality of our protocol is to include functionality in addition to pain relief as a primary outcome because these two endpoints are not linked in a linear way. For some patients, functional status is more important than pain relief.

Trial registration

EudraCT reference: 2020-000473-25,ClinicalTrials.gov: NCT04436250, first posted June 18, 2020; last updated July 21, 2020. Protocol version 2.2 issued on September 30, 2020, after a revision by the ethics committee.https://clinicaltrials.gov/ct2/show/NCT04436250
Synergistic effects of multiple enzymes from industrial Aspergillus niger strain O1 on starch saccharification
Authors: Guo Wenzhu , Yang Jianhua , Huang Tianchen , Liu Dandan , Liu Qian , Li Jingen , Sun Wenliang , Wang Xingji , Zhu Leilei , Tian Chaoguang
Published: November 2021

Background

Starch is one of the most important renewable polysaccharides in nature for production of bio-ethanol. The starch saccharification step facilitates the depolymerization of starch to yield glucose for biofuels production. The filamentous fungus Aspergillus niger (A. niger) is the most used microbial cell factory for production of the commercial glucoamylase. However, the role of each component in glucoamylases cocktail of A. niger O1 for starch saccharification remains unclear except glucoamylase.

Results

In this study, we identified the key enzymes contributing to the starch saccharification process are glucoamylase, α-amylase and acid α-amylase out of 29 glycoside hydrolases from the 6-day fermentation products of A. niger O1. Through the synergistic study of the multienzymes for the starch saccharification in vitro, we found that increasing the amount of α-amylase by 5-10 times enhanced the efficiency of starch saccharification by 14.2-23.2%. Overexpression of acid α-amylase in strain O1 in vivo increased the total glucoamylase activity of O1 cultures by 15.0%.

Conclusions

Our study clarifies the synergistic effects among the components of glucoamylases cocktail, and provides an effective approach to optimize the profile of saccharifying enzymes of strain O1 for improving the total glucoamylase activity.
Spawning behavior of Aedini (Diptera: Culicidae) in a remnant of Atlantic Forest in the state of Rio de Janeiro
Authors: Bastos Amanda Queiroz , Leite Paulo José , dos Santos-Mallet Jacenir Reis , de Mello Cecilia Ferreira , Serdeiro Michele , dos Silva Júlia Santos , Figueiró Ronaldo , Docile Tatiana , Alencar Jeronimo
Published: November 2021

Background

Mosquito assemblages are organized along an ecological gradient, including small habitats where interspecific competition predominates and large permanent habitats where predation predominates. This study aimed to analyze the oviposition behavior of mosquitoes regarding the preference for traps installed at two different heights with regard to ground level and the tendency to share spawning sites in an Atlantic Forest fragment in Nova Iguaçu, State of Rio de Janeiro, Brazil.

Methods

The eggs were collected from April 2018 to March 2019. Twelve ovitraps were used, randomly distributed in trees at ground level and at a height of 3 m in a forest environment.

Results

They were sequentially numbered, monitored, and replaced every 2 weeks. Among the 5818 eggs collected, 3941 hatched, 3756 reached the pupa stage, and 2370 reached the adult stage. The most abundant species wereAedes albopictus(63%) andHaemagogus leucocelaenus(35%), followed byAedes terrens(2%) andHaemagogus janthinomys(1%). Analyses showed a significant difference of (P = 0.02) between the number of mosquito species collected in the palettes at ground level and the number collected at the height of 3 m. Cluster analysis of species abundance showed that the eggs collected in the palettes at ground level were more abundant than those collected in the palettes at the height of 3 m. We detected co-occurrence of species in the oviposition palettes; according to the null model, such species distribution was not random.

Conclusions

The exploitation of oviposition sites by mosquito species can represent an event forced by population density facilitated by the ecological valence of individuals of one species. Understanding the aggregate distribution of larvae at the oviposition site allows us to conduct more in-depth studies of the oviposition behavior of female mosquitoes.

Graphical Abstract
Whole-genome analysis reveals the contribution of non-coding de novo transposon insertions to autism spectrum disorder
Authors: Borges-Monroy Rebeca , Chu Chong , Dias Caroline , Choi Jaejoon , Lee Soohyun , Gao Yue , Shin Taehwan , Park Peter J. , Walsh Christopher A. , Lee Eunjung Alice
Published: November 2021

Background

Retrotransposons have been implicated as causes of Mendelian disease, but their role in autism spectrum disorder (ASD) has not been systematically defined, because they are only called with adequate sensitivity from whole genome sequencing (WGS) data and a large enough cohort for this analysis has only recently become available.

Results

We analyzed WGS data from a cohort of 2288 ASD families from the Simons Simplex Collection by establishing a scalable computational pipeline for retrotransposon insertion detection. We report 86,154 polymorphic retrotransposon insertions—including > 60% not previously reported—and 158 de novo retrotransposition events. The overall burden of de novo events was similar between ASD individuals and unaffected siblings, with 1 de novo insertion per 29, 117, and 206 births for Alu, L1, and SVA respectively, and 1 de novo insertion per 21 births total. However, ASD cases showed more de novo L1 insertions than expected in ASD genes. Additionally, we observed exonic insertions in loss-of-function intolerant genes, including a likely pathogenic exonic insertion in CSDE1, only in ASD individuals.

Conclusions

These findings suggest a modest, but important, impact of intronic and exonic retrotransposon insertions in ASD, show the importance of WGS for their analysis, and highlight the utility of specific bioinformatic tools for high-throughput detection of retrotransposon insertions.
Optimizing high-flow nasal cannula flow settings in adult hypoxemic patients based on peak inspiratory flow during tidal breathing
Authors: Li Jie , Scott J. Brady , Fink James B. , Reed Brooke , Roca Oriol , Dhand Rajiv
Published: November 2021

Background

Optimal flow settings during high-flow nasal cannula (HFNC) therapy are unknown. We investigated the optimal flow settings during HFNC therapy based on breathing pattern and tidal inspiratory flows in patients with acute hypoxemic respiratory failure (AHRF).

Methods

We conducted a prospective clinical study in adult hypoxemic patients treated by HFNC with a fraction of inspired oxygen (FIO2) ≥ 0.4. Patient’s peak tidal inspiratory flow (PTIF) was measured and HFNC flows were set to match individual PTIF and then increased by 10 L/min every 5–10 min up to 60 L/min. FIO2 was titrated to maintain pulse oximetry (SpO2) of 90–97%. SpO2/FIO2, respiratory rate (RR), ROX index [(SpO2/FIO2)/RR], and patient comfort were recorded after 5–10 min on each setting. We also conducted an in vitro study to explore the relationship between the HFNC flows and the tracheal FIO2, peak inspiratory and expiratory pressures.

Results

Forty-nine patients aged 58.0 (SD 14.1) years were enrolled. At enrollment, HFNC flow was set at 45 (38, 50) L/min, with an FIO2 at 0.62 (0.16) to obtain an SpO2/FIO2 of 160 (40). Mean PTIF was 34 (9) L/min. An increase in HFNC flows up to two times of the individual patient’s PTIF, incrementally improved oxygenation but the ROX index plateaued with HFNC flows of 1.34–1.67 times the individual PTIF. In the in vitro study, when the HFNC flow was set higher than PTIF, tracheal peak inspiratory and expiratory pressures increased as HFNC flow increased but the FIO2 did not change.

Conclusion

Mean PTIF values in most patients with AHRF were between 30 and 40 L/min. We observed improvement in oxygenation with HFNC flows set above patient PTIF. Thus, a pragmatic approach to set optimal flows in patients with AHRF would be to initiate HFNC flow at 40 L/min and titrate the flow based on improvement in ROX index and patient tolerance.

Trial registration: ClinicalTrials.gov (NCT03738345). Registered on November 13th, 2018. https://clinicaltrials.gov/ct2/show/NCT03738345?term=NCT03738345&draw=2&rank=1
Research on college English teaching based on data mining technology
Authors: Duan Jinhui , Gao Rui
Published: November 2021

To improve the efficiency and quality of college English teaching, we analyzed the feasibility and application process of data mining technology in college English teaching. The entire process of data classification mining was fully realized. A new teaching program was proposed. The object and target of data mining were determined. Online surveys were used to collect data. Data integration, data cleaning, data conversion, data reduction and other pre-processing technologies were adopted. The decision tree was generated by using the C4.5 algorithm, and the pruning was carried out. The result analysis decision tree model was completed. A detailed survey of the students' English learning in University was made in detail. The results showed that the qualified rate of students' English performance was increased from 20–30% to 50–60%. Therefore, the classification rules provide theoretical support for the school teaching decision. This method can improve the quality of English teaching.
Biochemical characterization of a family IV esterase with R-form enantioselectivity from a compost metagenomic library
Authors: Park Jong Eun , Jeong Geum Seok , Lee Hyun Woo , Kim Hoon
Published: November 2021

A novel family IV esterase (hormone-sensitive lipase, HSL) gene, est15L, was isolated from a compost metagenomic library. Encoded Est15L comprised 328 amino acids with a molecular weight of 34,770 kDa and was an intracellular esterase without a signal peptide. The multiple sequence alignment (MSA) of Est15L with other family IV esterases showed conserved regions such as HGG, DYR, GXSXG, DPL, and GXIH. Native Est15L was a dimeric form from the results of size exclusion chromatography. It was optimally active at 50 ℃ and pH 9.0, indicating alkaline esterase. However, it showed a low thermostability with half-lives of 30.3 at 30 ℃ and 2.7 min at 40 ℃. It preferred p-nitrophenyl butyrate (C4) with Km and Vmax values of 0.28 mM and 270.8 U/mg, respectively. Est15L was inhibited by organic solvents such as 30% methanol, isopropanol, and acetonitrile with residual activities of 12.5, 0.9, and 0.3%, respectively. It was also inhibited by 1% SDS and 1% PMSF; however, Est15L maintained its activity at 1% Triton X-100 and EDTA. Est15L was inhibited by Cu2+, Zn2+, Mn2+, Co2+, Fe2+, and Na+. In addition, Est15L hydrolyzed glyceryl tributyrate with a residual substrate amount of 43.7% at 60 min but could not hydrolyze the oils (fish and olive) and glyceryl trioleate. Interestingly, Est15L showed significant enantioselectivity toward the R-form with a residual substrate amount of 44.6%, lower than that of the S-form (83.5%). Considering its properties, Est15L can be a potential candidate for chemical reactions, such as the synthesis of pharmaceutical compounds.
Investigating course choice motivations in university environments
Authors: Ma Boxuan , Lu Min , Taniguchi Yuta , Konomi Shin’ichi
Published: November 2021

Recommendation systems need a deeper understanding of users and their motivations to improve recommendation quality and provide more personalized suggestions. This is especially true in the education domain, the more about the student is known, the more useful recommendations can be made. However, although many studies on the course recommendation exist, studies on the students’ course selection motivations in universities are limited. This study investigates the factors that contribute to students’ choice when selecting courses in universities to better understand student perceptions, attitudes, and needs and leverage data-driven approaches for recommending and explaining the recommendations in university environments. A qualitative interview for university students (N = 10) comprised of open-ended questions as well as a questionnaire for students (N = 81) was conducted, aiming to investigate the main reasons behind their choices. The results of this study show that students highly value the course contents and the benefits of the course towards their future careers. Furthermore, students are influenced by other reasons such as the possibility of obtaining a higher grade, the popularity of professors, and recommendations from peers. Next, we extract the main categories of students’ motivations and analyzed the questionnaire data by employing statistical analysis methods as well as the k-means clustering algorithm to identify different types of students in terms of course selection. Based on our findings, we discuss implications for designing more personalized course recommendation systems.
Peripheral refraction of myopic eyes with spectacle lenses correction and lens free emmetropes during accommodation
Authors: Queirós António , Cerviño Alejandro , González-Méijome José Manuel
Published: December 2021

Purpose

To measure axial and off-axis refraction patterns in myopic eyes with spectacle lenses correction and lens free emmetropes in young healthy subjects at different target distances from 2.00 m (0.50 D) to 0.20 m (5.00 D) in terms of sphere, astigmatism, and spherical equivalent refraction.

Methods

Refraction was measured at the center, 20 and 40 degrees from the line of sight both nasally and temporally in 15 emmetropic and 25 myopic young healthy subjects with an open field, binocular, infrared autorefractor (Grand Seiko WAM-5500, Hiroshima, Japan). Fixation target was a Maltese cross set at 2.00, 0.50, 0.33 and 0.20 m from the corneal plane. Changes in off-axis refraction with accommodation level were normalized with respect to distance axial values and compared between myopic eyes with spectacle lenses correction and lens free emmetropes.

Results

Off-axis refraction in myopic eyes with spectacle lenses correction was significantly more myopic in the temporal retina compared to lens free emmetropes except for the closest target distance. Relative off-axis refractive error changed significantly with accommodation when compared to axial refraction particularly in the myopic group. This change in the negative direction was due to changes in the spherical component of refraction that became more myopic relative to the center at the 0.20 m distance as the J0 component of astigmatism was significantly reduced in both emmetropes and myopes for the closest target.

Conclusion

Accommodation to very near targets (up to 0.20 m) makes the off-axis refraction of myopes wearing their spectacle correction similar to that of lens free emmetropes. A significant reduction in off-axis astigmatism was also observed for the 0.20 m distance.
Determination of representative elementary volume (REV) for jointed rock masses exhibiting scale-dependent behavior: a numerical investigation
Authors: Sari Mehmet
Published: December 2021

Representative elementary volume (REV) is defined as the usual size of a rock mass structure beyond which its mechanical properties are homogenous and isotropic, and its behavior can be modeled using the equivalent continuum approach. Determination of REV is a complex problem in rock engineering due to its definition ambiguity and application area. This study is one of the first attempts to define a REV for jointed rock masses using the equivalent continuum approach. It is aimed to numerically search a ratio between the characteristic size of an engineering structure and pre-existing joint spacing, which are the two most important contributing elements in assessing REV. For this purpose, four hypothetical engineering cases were investigated using the RS2 (Phase2 v. 9.0) finite element (FE) analysis program. An underground circular opening with a constant diameter, an open-pit mine with varying bench heights, a single bench with a constant height, and an underground powerhouse cavern with a known dimension were executed for possible changes in the safety factor and total displacement measurements under several joint spacing values. Different cut-off REVs were calculated for FE models depending on the type of excavation and measurement method. An average REV size of 19.0, ranging between a minimum of 2 for tunnels and a maximum of 48 for slopes, was found in numerical analysis. The calculated sizes of REV were significantly larger than the range of values (5 to 10) commonly reported in the relevant geotechnical literature.
Potential drug–drug interactions in the era of integrase strand transfer inhibitors: a cross-sectional single-center study in Japan
Authors: Kunimoto Yusuke , Matamura Ryosuke , Ikeda Hiroshi , Fujii Satoshi , Kimyo Tomoko , Kitagawa Manabu , Nakata Hiromasa , Kobune Masayoshi , Miyamoto Atsushi , Fukudo Masahide
Published: December 2021

Background

Potential drug–drug interactions (PDDIs) commonly occur because of aging and comorbidities in people living with human immunodeficiency virus (HIV; PLWH). Protease inhibitors and non-nucleoside reverse transcriptase inhibitors have been reported to cause PDDIs in these patients. However, there are few reports of PDDIs in the era of treatment using integrase strand transfer inhibitors. Therefore, we investigated PDDIs in Japanese PLWH receiving antiretroviral drugs (ARVs).

Methods

This was a cross-sectional observational study conducted in Japanese outpatients. All eligible patients who had received ARV therapy for at least 48 weeks were enrolled. The primary endpoint was the incidence of PDDIs detected using the Lexicomp® interface.

Results

Of the 71 eligible patients, 51 (71.8%) were prescribed concomitant non-ARV medications. In 21 patients (29.6%), PDDIs with the potential to reduce the effects of ARVs occurred, although the HIV load was suppressed in all cases. Polypharmacy (the use of ≥5 non-ARVs) was observed in 25 patients (35.2%). There was a significantly higher median number of non-ARV medications in the PDDI group than in the non-PDDI group (6 vs. 3, P <  0.001). Furthermore, the proportion of patients on polypharmacy was significantly higher in those with PDDIs than in those without PDDIs (81.0% vs. 26.7%, P <  0.001).

Conclusions

The incidence of PDDIs is relatively high in Japanese PLWH, even in the era of treatment using integrase strand transfer inhibitors. Therefore, it is important for patients and health care providers to be constantly aware of PDDIs associated with ARV treatment.
Constructing and validating a questionnaire on barriers to EFL learners’ reflective writing
Authors: Salahi Fariba , Farahian Majid
Published: December 2021

The purpose of the current study was to develop and validate a scale to assess the inhibitors to reflective writing of Iranian English as a Foreign Language (EFL) learners. The other aim of this study was to assess the reliability of the questionnaire. To prepare the first item pool of the questionnaire, the researchers reviewed the previous related literature and prepared the first item pool with 62 items. At the next stage, based on the judgments of some experts in the field regarding the items of the questionnaire, 31 items were deleted and the next draft included 31 items. Then, 265 EFL teachers were invited to fill out the questionnaire. As the next step, exploratory factor analysis (EFA) was conducted to investigate the construct validity of the scale. Based on the results of EFA, 10 items were deleted. The final draft of the questionnaire included 21 items with three factors including ‘teachers’ issues’, ‘students’ issues’, and ‘institutions and educational system issues’. In addition, a Cronbach’s alpha coefficient was run to measure the internal consistency of the scale and the findings indicated a satisfactory level of reliability. The findings have implications for pedagogy as well as further research.
Underlying mechanism of hemodynamics and intracranial aneurysm
Authors: Tang Haishuang , Wang Qingsong , Xu Fengfeng , Zhang Xiaoxi , Zeng Zhangwei , Yan Yazhou , Lu Zhiwen , Xue Gaici , Zuo Qiao , Luo Yin , Liu Jianmin , Huang Qinghai
Published: December 2021

In modern society, subarachnoid hemorrhage, mostly caused by intracranial aneurysm rupture, is accompanied by high disability and mortality rate, which has become a major threat to human health. Till now, the etiology of intracranial aneurysm has not been entirely clarified. In recent years, more and more studies focus on the relationship between hemodynamics and intracranial aneurysm. Under the physiological condition, the mechanical force produced by the stable blood flow in the blood vessels keeps balance with the structure of the blood vessels. When the blood vessels are stimulated by the continuous abnormal blood flow, the functional structure of the blood vessels changes, which becomes the pathophysiological basis of the inflammation and atherosclerosis of the blood vessels and further promotes the occurrence and development of the intracranial aneurysm. This review will focus on the relationship between hemodynamics and intracranial aneurysms, will discuss the mechanism of occurrence and development of intracranial aneurysms, and will provide a new perspective for the research and treatment of intracranial aneurysms.
Gender disparity in publication records: a qualitative study of women researchers in computing and engineering
Authors: Hosseini Mohammad , Sharifzad Shiva
Published: December 2021

Background

The current paper follows up on the results of an exploratory quantitative analysis that compared the publication and citation records of men and women researchers affiliated with the Faculty of Computing and Engineering at Dublin City University (DCU) in Ireland. Quantitative analysis of publications between 2013 and 2018 showed that women researchers had fewer publications, received fewer citations per person, and participated less often in international collaborations. Given the significance of publications for pursuing an academic career, we used qualitative methods to understand these differences and explore factors that, according to women researchers, have contributed to this disparity.

Methods

Sixteen women researchers from DCU’s Faculty of Computing and Engineering were interviewed using a semi-structured questionnaire. Once interviews were transcribed and anonymised, they were coded by both authors in two rounds using an inductive approach.

Results

Interviewed women believed that their opportunities for research engagement and research funding, collaborations, publications and promotions are negatively impacted by gender roles, implicit gender biases, their own high professional standards, family responsibilities, nationality and negative perceptions of their expertise and accomplishments.

Conclusions

Our study has found that women in DCU’s Faculty of Computing and Engineering face challenges that, according to those interviewed, negatively affect their engagement in various research activities, and, therefore, have contributed to their lower publication record. We suggest that while affirmative programmes aiming to correct disparities are necessary, they are more likely to  improve organisational culture if they are implemented in parallel with bottom-up initiatives that engage all parties, including men researchers and non-academic partners, to inform and sensitise them about the significance of gender equity.
Meeting Report: Vaccine Stability Considerations to Enable Rapid Development and Deployment
Authors: Alasandro Mark , Choudhury Dilip , Huynh-Ba Kim , Kochling Jianmei , Latoz Christopher , Larkin Laure , McCaig Lori , Subbarao Nanda , Wu Yan , Zhang Yajie
Published: December 2021

The Stability Community of the American Association of Pharmaceutical Scientists (AAPS) held a virtual workshop on “Vaccine Stability Considerations to Enable Rapid Development and Deployment”, on March 24-25, 2021. The workshop included distinguished speakers and panelists from across the industry, academia, regulatory agencies, as well as health care leaders. This paper presents a review of the topics covered. Specifically the challenges in accelerating vaccine development and analytical characterization techniques to establish shelf-life were covered. Additionally, vaccine stability modeling using prior knowledge stability models and advanced kinetic analysis played a key in the EUA approaches discussed during the workshop. Finally, the role of stability studies in addressing the challenges of vaccine distribution and deployment during the pandemic were a focus of presentations and panel discussions.

Although the workshop did not have any presentation topics directly dedicated to the mRNA vaccines, the techniques discussed are generally applicable. The mRNA vaccine developers were represented in the panel discussions, where experts involved in the EUA approval/deployment stages for this vaccine type could discuss the challenges as applied to their vaccines.
An alternate novel approach to classify lip prints
Authors: Kaur Jagmeet , Thakar Mukesh Kumar
Published: November 2021

Background

The wrinkles and grooves present on the sulci labiorum of human lips form characteristic patterns. As previous authors claimed, these can be used for personal identification in forensic cases because they are unique, permanent, and classifiable. Many scientists had devised different systems to classify the lip prints (Suzuki and Tsuchihashi, Martin Santos, Renaud, Afchar Bayar, and Jose Maria Dominguez). These systems were based solely on the shape of patterns present on the whole lip. However, sometimes, lip prints affected by either habitual smoking or any other pathological conditions are encountered. The investigator may have to form an opinion on partial lip print to identify the culprit. Therefore, in the present study, an alternative comprehensive system to classify even the partial prints has been proposed. Lip print samples were collected from 500 individuals (400 females and 100 males, respectively) using the tape lifting method. The whole lip print (Klein’s zone) was divided into 10 quadrants (five each on upper and lower lips) to study the whole pattern area thoroughly. The sample-wise and quadrant-wise frequency of all the patterns was statistically calculated. Sexual dimorphism of the lip print patterns was also assessed statistically.

Results

The method used emphasizes the consistency of patterns, which have been divided into Basic and Combination Patterns. Detailed examination of lip prints revealed that all the quadrants tend to have 7 types of Basic Patterns and 43 types of Combination Patterns despite similar appearance. These patterns can be further categorized into various subtypes based on the direction and orientation of wrinkle lines constituting the original pattern. Most of the Basic and Combination Patterns were widely distributed in all the analysed lip print samples and showed significant dependence on the sex of the subject.

Conclusions

The lip print patterns examined in the present study can be useful as an alternative to the existing systems to classify even partial lip marks recovered at the crime scene.
Clinical and morphological features of SARS-COV-2 associated acute hemorrhagic necrotizing encephalopathy: case report
Authors: Ermilov Victor Vladimirovich , Dorofeev Nikita Alexeevich
Published: November 2021

Background

The current case report presents acute hemorrhagic necrotizing encephalopathy (AHNE) as an example of a fatal complication, the etiology of which could be coronavirus disease 2019 (COVID-19) with multiple organ damage along with the existing respiratory tuberculosis.

Case presentation

A male in his 20s had severe symptoms of central nervous system lesion, which developed against the background of COVID-19 and respiratory tuberculosis, for which he was treated in the intensive care unit. Autopsy confirmed that he died from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) associated AHNE in adults with severe fatal endothelial dysfunction and respiratory tuberculosis. The main morphological signs of brain damage were desquamative endotheliitis, thrombosis, parenchymal hemorrhagic necrosis, encephalitis, severe necrobiotic neuronal damage.

Conclusion

The defeat of endothelial cells with the development of generalized endotheliitis in COVID-19, especially in conjunction with comorbid pathology, in particular tuberculosis, can lead to a fatal complication that affects the nervous system—AHNE. Therefore, it is worth paying close attention to the appearance of neurological symptoms in patients with a similar combination of diseases.
A semi-systematic review on hypertension and dyslipidemia care in Egypt—highlighting evidence gaps and recommendations for better patient outcomes
Authors: Reda Ashraf , Ragy Hany , Saeed Kanwal , Alhussaini Mohammed Ashraf
Published: December 2021

Background

Both hypertension and dyslipidemia are considered as major modifiable risk factors of cardiovascular diseases (CVDs), and their prevalence in Egypt has increased in recent years. Evidence-based systematic evaluation of data on hypertension and dyslipidemia is critical for effective patient-centric management to reduce the overall risk of CVDs in Egypt. This semi-systematic review aimed to quantify and identify data gaps in the prevalence and distribution of patient journey touchpoints including awareness, screening, diagnosis, treatment, adherence, and control of hypertension and dyslipidemia to provide the basis for research prioritization, practice guidance, and health care reforms in Egypt.

Main body

Structured search was conducted on MEDLINE and Embase to identify articles published in English between January 2010 and December 2019 that reported key patient journey touchpoints in hypertension and dyslipidemia management. Unstructured search was conducted on public or government websites with no date restriction. Data from all sources were extracted and presented descriptively. In total, 22 studies published between 1995 and 2020 on hypertension and dyslipidemia were included in the final analyses. The prevalence of hypertension in Egypt ranged from 12.1 to 59%. Studies reported awareness (37.5% and 43.9%), diagnosis (42% and 64.7%), treatment (24% and 54.1%), and adherence to antihypertensive medication (51.9%) to be low. Furthermore, the percentage of patients who had their blood pressure controlled ranged from 8 to 53.2%. The prevalence of dyslipidemia varied in the general population (range 19.2–36.8%) but was higher in patients with acute coronary syndrome (ACS) (50.9% and 52.5%) and coronary artery disease (58.7%). A national report indicated that 8.6% of the general population was screened for dyslipidemia; however, no data was available on the diagnosis and treatment rates. Among ACS patients, 73.9% were treated for dyslipidemia. Data indicated low levels of medication adherence (59%) among dyslipidemia patients, with overall low control rates ranging from 5.1 to 34.4% depending on CVD risk in populations including ACS patients.

Conclusion

Data on patient journey touchpoints of hypertension and dyslipidemia are limited in Egypt, indicating the need for more systematic and high-quality evidence-based studies covering different aspects of patient-centric management for better management of CVD and its risk factors.
A new process on the basic formula of kimchi: derived kimchi from a combination of yangnyeom (kimchi sauce) and vegetables
Authors: Lee Chang Hyeon , Ko Young Ju
Published: November 2021

This study proposes a way of reconsidering how to explain kimchi, which is quite diverse even though it is often referred to as if it expresses a single, universal dish, to non-Koreans, particularly given the worldwide rise in its popularity. Koreans are accustomed to the general process of explaining kimchi in terms of the different types of vegetables that are the main ingredients. However, this process makes it difficult for Westerners who are new to kimchi to fully understand the comprehensive meaning and value of the dish. For this reason, it is time to change the process of explaining kimchi for Westerners who are accustomed to food culture using sauces. We believe that our study makes a significant contribution to the literature and will be of interest to the readership of the Journal of Ethnic Foods journal because it reviews and draws from 15 kimchi recipes posted by the world-famous culinary YouTuber Maangchi and proposes that discussing and classifying the composition and structure of ingredients and the basic formula as the best way of introducing non-Koreans to the variety of this quintessentially Korean dish and encouraging them to create their own varieties of kimchi upon a simple foundation of ingredients. People worldwide will be able to evolve to the stage where they can recognize and reconstruct the basic formula in which various derived kimchi types are made by combining kimchi yangnyeom and vegetables.
User experience and motivation with engineering design challenges in general chemistry laboratory
Authors: Crippen Kent J. , Imperial Lorelie , Payne Corey , Bolch Charlotte A. , Korolev Maria , Wu Chang-Yu , Brucat Philip
Published: November 2021

Our career-forward approach to general chemistry laboratory for engineers involves the use of design challenges (DCs), an innovation that employs authentic professional context and practice to transform traditional tasks into developmentally appropriate career experiences. These challenges are scaled-down engineering problems related to the US National Academy of Engineering’s Grand Challenges that engage students in collaborative problem solving via the modeling process. With task features aligned with professional engineering practice, DCs are hypothesized to support student motivation for the task as well as for the profession. As an evaluation of our curriculum design process, we use expectancy–value theory to test our hypotheses by investigating the association between students’ task value beliefs and self-confidence with their user experience, gender and URM status. Using stepwise multiple regression analysis, the results reveal that students find value in completing a DC (F(5,2430) = 534.96, p < .001) and are self-confident (F(8,2427) = 154.86, p < .001) when they feel like an engineer, are satisfied, perceive collaboration, are provided help from a teaching assistant, and the tasks are not too difficult. We highlight that although female and URM students felt less self-confidence in completing a DC, these feelings were moderated by their perceptions of feeling like an engineer and collaboration in the learning process (F(10,2425) = 127.06, p < .001). When female students felt like they were engineers (gender x feel like an engineer), their self-confidence increased (β = .288) and when URM students perceived tasks as collaborative (URM status x collaboration), their self-confidence increased (β = .302). Given the lack of representation for certain groups in engineering, this study suggests that providing an opportunity for collaboration and promoting a sense of professional identity afford a more inclusive learning experience.
Nutritious mushroom protein crisp – healthy alternative to starchy snack
Authors: Balan Venkatesh , Novak Dianne , Knudson William , Jones A. Daniel , Iñiguez-Franco Fabiola Maria , Auras Rafael , Cho Sungeun , Rodgers Amanda , Ubanwa Bryan
Published: December 2021

Global salty snacks category had reached USD 137 billion in sales in 2018. Due to growing health concerns and awareness, consumers are looking for healthy snack choices by avoiding ingredients such as fat, sugar, cholesterol, and sodium and selecting baked and salt free multigrain chips. A sizable number of consumers are concerned about snack nutrition and look for quality ingredients and minimally processed foods called as “Good Health Snack (GHS)”. In this work, we present the development of method of producing and testing mushrooms protein crisps (MPC), a healthy alternative to conventional starchy snacks that are rich in protein, nutraceutical compounds, minerals, vitamin, dietary fiber, and immunity inducing beta-glucans. The methods of producing MPC with different seasoning and hydrolyzed protein, calorie, nutritional and chemical composition, consumer response, shelf life after packing and market analysis are described. These systematic studies will help to market potential of this product which is a healthy alternative to other calorie rich snacks sold in the market benefiting the consumers.

Graphical abstract
Combined presentation of severe pulmonary COVID-19 and multisystem inflammatory syndrome in children (MIS-C): two reported Egyptian pediatric cases
Authors: Hamed Tarek , Sarhan Dina T.
Published: December 2021

Background

Initial reports from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic described children as being less susceptible to coronavirus disease (COVID-19) than adults. Later on, a severe and novel pediatric disorder termed multisystem inflammatory syndrome in children (MIS-C) emerged. Pediatric patients with SARS-CoV-2 are at risk for critical illness with severe pulmonary COVID-19 and MIS-C. Both are described as two distinct conditions, and the differentiation between them was the scope of many studies. In this report from Egypt, we will describe two unique pediatric cases presented by combined manifestations of severe pulmonary COVID-19 and MIS-C.

Case presentation

Two patients presented with severe pulmonary COVID-19 evident by pulmonary symptoms, signs, and advanced CO-RADS stage in lung CT were simultaneously fulfilling the clinical criteria of MIS-C including fever, multi-system affection, increased inflammatory markers in addition to the proved COVID-19 by positive serologic tests for SARS-CoV-2 but PCR was negative. Both patients responded well to immune-modulation therapy by IVIG and steroids and discharged well under closed follow-up.

Conclusions

Although it is debatable to present simultaneously, MIS-C should be considered in patients presenting with typical clinical findings and concerns for pulmonary COVID-19 once the criteria for MIS-C diagnosis is fulfilled. Starting treatment without delay can favor better prognosis.
Signal-to-noise ratio uniformity and stability of agar gel phantom with iron (III) oxide as relaxation modifier
Authors: Er W. X. , Lim W. J. , Dwihapsari Y. , Awang M. N. A. , Yusoff A. N.
Published: November 2021

Background

Agar has been commonly used as one of the materials to fabricate magnetic resonance imaging phantoms in the past few decades. In this study, eleven agar gel phantoms with different iron (III) oxide (Fe2O3) masses were prepared. This study was aimed to evaluate the signal-to-noise ratio (SNR) uniformity and stability of agar gel phantoms with and without the addition of Fe2O3 at two different time points (TPs). Fe2O3 powder was used as a relaxation modifier to manipulate and produce various SNR, T1 and T2 values. These phantoms were scanned using turbo spin echo pulse sequence to produce T1- and T2-measurement images. The SNR was then computed by plotting 1, 3 and 25 regions of interest on the images using ImageJ software. The T1 and T2 relaxation equations were then fitted to the experimental results of SNR versus TR and SNR versus TE curves for the determination of saturation (SNRo), T1 and T2 values.

Results

The results demonstrated that the agar gel phantoms were able to maintain SNR uniformity but not SNR stability after 4 weeks of phantom preparation. The change in the water content and microstructure of the phantoms have no significant effect on T2 relaxation but on T1 relaxation. The T1 and T2 of the agar gel phantoms were minimally affected although there was a systemic increase in the content of the Fe2O3 powder.

Conclusions

It can be concluded that the agar gel phantoms exhibited the characteristics of SNR uniformity, but they showed instability of SNR at TP2. The Fe2O3 in powder form is not an effective relaxation modifier to reduce the T1 and T2 when it is introduced into the agar gel phantoms. Dissolved nanosized particles should be the focus of future studies.