bluuebunny/crossref_metadata_2025 huggingface dataset:
Process Steps for High Quality Si-Based Epitaxial Growth at Low Temperature via RPCVD
Authors: Jung Jongwan , Son Baegmo , Kam Byungmin , Joh Yong Sang , Jeong Woonyoung , Cho Seongjae , Lee Won-Jun , Park Sangjoon
Published: July 2021

The key process steps for growing high-quality Si-based epitaxial films via reduced pressure chemical vapor deposition (RPCVD) are investigated herein. The quality of the epitaxial films is largely affected by the following steps in the epitaxy process: ex-situ cleaning, in-situ bake, and loading conditions such as the temperature and gaseous environment. With respect to ex-situ cleaning, dry cleaning is found to be more effective than wet cleaning in 1:200 dilute hydrofluoric acid (DHF), while wet cleaning in 1:30 DHF is the least effective. However, the best results of all are obtained via a combination of wet and dry cleaning. With respect to in-situ hydrogen bake in the presence of H2 gas, the level of impurities is gradually decreased as the temperature increases from 700 °C to a maximum of 850 °C, at which no peaks of O and F are observed. Further, the addition of a hydrogen chloride (HCl) bake step after the H2 bake results in effective in-situ bake even at temperatures as low as 700 °C. In addition, the effects of temperature and environment (vacuum or gas) at the time of loading the wafers into the process chamber are compared. Better quality epitaxial films are obtained when the samples are loaded into the process chamber at low temperature in a gaseous environment. These results indicate that the epitaxial conditions must be carefully tuned and controlled in order to achieve high-quality epitaxial growth.
Impact of Cross-Linking of Collagen Matrices on Tissue Regeneration in a Rabbit Calvarial Bone Defect
Authors: Fujioka-Kobayashi Masako , Andrejova Elena , Katagiri Hiroki , Schaller Benoit , Sculean Anton , Imber Jean-Claude , Lang Niklaus P. , Saulacic Nikola
Published: July 2021

The cross-linking of collagen matrices (Cl_CM) may provide volume-stable enhanced defect regeneration when compared to non-cross-linked matrices (Ncl_CM). The aim of the present study was to investigate the bone forming potential of collagen matrices (CMs) and the effects of cross-linking CMs in a rabbit calvaria defect model. (1) Empty controls (n = 6), (2) Ncl_CM (n = 8), and (3) Cl_CM (n = 8) were selected to be observed for the healing in 10 mm critical-sized calvarial bone defects. The potential for the bone as well as the connective tissue formation were evaluated by micro-CT and histomorphometry at three months post-surgery. There were no statistically significant differences in terms of new bone volume in the defects between the groups. However, the Cl_CM induced significantly greater fibrous tissue regeneration (5.29 ± 1.57 mm2) when compared to the controls (3.51 ± 0.93 mm2) by histomorphometry. The remnants of collagen fibers with immune cells, including macrophages and giant cells, were occasionally observed in the Cl_CM group but not in the Ncl_CM group. In conclusion, the cross-linking of collagen did not influence the potential for bone formation. Nevertheless, Cl_CM might be advantageous for the maintenance of fibrous tissue volume without disturbing bone formation in the defects.
The Durability of Mortar Containing Alkali Activated Fly Ash-Based Lightweight Aggregate
Authors: Risdanareni Puput , Van den Heede Philip , Wang Jianyun , De Belie Nele
Published: July 2021

Beneficiating fly ash as valuable construction material such as artificial lightweight aggregate (LWA) could be an alternative solution to increase the utilization of the industrial by-product. However, generally, LWA is characterized by high porosity and a related high water absorption, which on the one hand allows production of lightweight mortar, but on the other hand can affect its performance. Thus, in this research, the durability performance of mortar composed with alkali-activated fly ash-based LWA, and commercial expanded clay (EC) LWA was investigated. The fly ash LWA was prepared in a pan granulator, with a 6-molar solution of NaOH mixed with Na2SiO3 in a Na2SiO3/NaOH weight ratio of 1.5 being used as activator (FA 6M LWA). The results revealed that mortar containing FA 6M LWA had equivalent mechanical strength with mortar containing EC LWA. The mortar containing FA 6M LWA had comparable capillary water uptake and chloride migration resistance with the reference and EC LWA mortar. Furthermore, the addition of FA 6M LWA was proven to enhance the carbonation resistance in the resulting mortar, due to the denser interfacial transition zone (ITZ) of mortar with LWA.
Research on Improving NVH Performance of Automobile Side Door Glass in Use Based on 6 Sigma Method
Authors: Ma Chunlong , Shi Dongyan , Wang Mengnan , He Dongze , Li Chao , Yu Xingsheng
Published: July 2021

Automobile side door glass squeaks and rattles during use. This abnormal noise can make the driver and occupants irritable and reduce the comfort of the automobile. This reduces the sales of this automobile. This paper analyzes and determines the cause of squeak and rattle during the lifting and lowering process of the side door glass of an automobile. The noise is due to four reasons. One is that the distance between the inner waterproof belt and the automobile side door glass of the automobile is unreasonable, causing excessive friction between the automobile side door glass and the inner waterproof belt during the automobile side door glass up and down movement. Other factors affecting squeak and rattle may be the distance between the automobile side door sheet metal and the automobile side door glass, the thickness of the automobile side door glass and the characteristics of the inner waterproof belt. The first two dimensions are analyzed using the 6 sigma method, and the structure of the inner waterproof belt is improved and the flocking position is adjusted. The squeak and rattle phenomenon is explained using the implicit dynamic analysis method ABAQUS, and the compression load deflection after the installation of the inner waterproof belt is 3–9 N/100 mm. This research completely solves the squeak and rattle problem caused by the up and down movement of the side door glass of the automobile. This research has significance for solving practical engineering problems.
Experimental Investigation on Thermal Conductivity and Thermal Diffusivity of Ex-Vivo Bovine Liver from Room Temperature down to −60 °C
Authors: Campagnoli Elena , Giaretto Valter
Published: July 2021

Ex vivo animal tissues (e.g., bovine liver) as well as water-agar gel are commonly used to simulate both experimentally and numerically the response of human tissues to cryoablation treatments. Data on the low temperature thermal properties of bovine liver are difficult to find in the literature and very often are not provided for the whole temperature range of interest. This article presents the thermal conductivity and thermal diffusivity measurements performed on ex-vivo bovine liver samples using the transient plane source method. Regression coefficients are provided to determine these properties in different temperature ranges except for the phase transition during which no results were obtained, which suggests an ad hoc calorimetric analysis. A quick procedure is also suggested to determine the water mass fraction in the tissue. Moreover, an attempt to estimate the liver density in the frozen state using measurements performed solely at room temperature is also presented. The measured thermal conductivity and thermal diffusivity values are compared with data reported in literature highlighting a spread up to 40%. Moreover, it emerges that water-agar gel usually made with 2% by weight of agar does not show the same thermal properties as the bovine liver.
Chemical Vapor Deposition of N-Doped Graphene through Pre-Implantation of Nitrogen Ions for Long-Term Protection of Copper
Authors: Han Luoqiao , Dong Lei , Chen Haiyan , Yang Shuai , Yuan Aiheng , Guan Ran , Yan Hong , Wu Jing , Zhang Bo , Li Dejun , Luo Birong
Published: July 2021

Nitrogen-doped graphene (NG) was synthesized through the chemical vapor deposition (CVD) of graphene on Cu substrates, which were pre-implanted with N ions by the ion implantation method. The pre-implanted N ions in the Cu substrate could dope graphene by the substitution of C atoms during the CVD growth of graphene, forming NG. Based on this, NG’s long-term protection properties for Cu were evaluated by ambient exposure for a corrosion test. The results showed that NG can obviously reduce the natural oxidation of Cu in the long-term exposure compared with the case of pristine graphene (PG) coated on Cu. Moreover, with the increase in pre-implanted N dose, the formed NG’s long-term protection for Cu improved. This indicates that the modification of graphene by N doping is an effective way to improve the corrosion resistance of the PG coating owing to the reduction in its conductivity, which would inhibit galvanic corrosion by cutting off electron transport across the interface in their long-term protection. These findings provide insight into corrosion mechanisms of the graphene coating and correlate with its conductive nature based on heteroatoms doping, which is a potential route for improving the corrosion resistance of graphene as an effective barrier coating for metals.
A Multi-Depot Vehicle Routing Problem with Stochastic Road Capacity and Reduced Two-Stage Stochastic Integer Linear Programming Models for Rollout Algorithm
Authors: Anuar Wadi Khalid , Lee Lai Soon , Seow Hsin-Vonn , Pickl Stefan
Published: July 2021

A matheuristic approach based on a reduced two-stage Stochastic Integer Linear Programming (SILP) model is presented. The proposed approach is suitable for obtaining a policy constructed dynamically on the go during the rollout algorithm. The rollout algorithm is part of the Approximate Dynamic Programming (ADP) lookahead solution approach for a Markov Decision Processes (MDP) framed Multi-Depot Dynamic Vehicle Routing Problem with Stochastic Road Capacity (MDDVRPSRC). First, a Deterministic Multi-Depot VRP with Road Capacity (D-MDVRPRC) is presented. Then an extension, MDVRPSRC-2S, is presented as an offline two-stage SILP model of the MDDVRPSRC. These models are validated using small simulated instances with CPLEX. Next, two reduced versions of the MDVRPSRC-2S model (MDVRPSRC-2S1 and MDVRPSRC-2S2) are derived. They have a specific task in routing: replenishment and delivering supplies. These reduced models are to be utilised interchangeably depending on the capacity of the vehicle, and repeatedly during the execution of rollout in reinforcement learning. As a result, it is shown that a base policy consisting of an exact optimal decision at each decision epoch can be obtained constructively through these reduced two-stage stochastic integer linear programming models. The results obtained from the resulting rollout policy with CPLEX execution during rollout are also presented to validate the reduced model and the matheuristic algorithm. This approach is proposed as a simple implementation when performing rollout for the lookahead approach in ADP.
Application of Induced Preorderings in Score Function-Based Method for Solving Decision-Making with Interval-Valued Fuzzy Soft Information
Authors: Ali Mabruka , Kiliçman Adem , Zahedi Khameneh Azadeh
Published: July 2021

Ranking interval-valued fuzzy soft sets is an increasingly important research issue in decision making, and provides support for decision makers in order to select the optimal alternative under an uncertain environment. Currently, there are three interval-valued fuzzy soft set-based decision-making algorithms in the literature. However, these algorithms are not able to overcome the issue of comparable alternatives and, in fact, might be ignored due to the lack of a comprehensive priority approach. In order to provide a partial solution to this problem, we present a group decision-making solution which is based on a preference relationship of interval-valued fuzzy soft information. Further, corresponding to each parameter, two crisp topological spaces, namely, lower topology and upper topology, are introduced based on the interval-valued fuzzy soft topology. Then, using the preorder relation on a topological space, a score function-based ranking system is also defined to design an adjustable multi-steps algorithm. Finally, some illustrative examples are given to compare the effectiveness of the present approach with some existing methods.
An Embedding Strategy Using Q-Ary Convolutional Codes for Large and Small Payloads
Authors: Wang Jyun-Jie , Lin Chi-Yuan , Yang Sheng-Chih , Chang Hsi-Yuan , Lin Yin-Chen
Published: July 2021

Matrix embedding (ME) code is a commonly used steganography technique, which uses linear block codes to improve embedding efficiency. However, its main disadvantage is the inability to perform maximum likelihood decoding due to the high complexity of decoding large ME codes. As such, it is difficult to improve the embedding efficiency. The proposed q-ary embedding code can provide excellent embedding efficiency and is suitable for various embedding rates (large and small payloads). This article discusses that by using perforation technology, a convolutional code with a high embedding rate can be easily converted into a convolutional code with a low embedding rate. By keeping the embedding rate of the (2, 1) convolutional code unchanged, convolutional codes with different embedding rates can be designed through puncturing.
Short-Term Effects of a Conditioning Telerehabilitation Program in Confined Patients Affected by COVID-19 in the Acute Phase. A Pilot Randomized Controlled Trial
Authors: Rodriguez-Blanco Cleofas , Gonzalez-Gerez Juan Jose , Bernal-Utrera Carlos , Anarte-Lazo Ernesto , Perez-Ale Manuel , Saavedra-Hernandez Manuel
Published: July 2021

Background and objectives: The COVID-19 pandemic has become a challenge for health systems and, specifically, to physical therapists obligated to adapt their job and stop face-to-face consultations. In this situation, therapeutic exercise has been implemented in different COVID-19 patients. This study evaluated the feasibility and effectiveness of a novel therapeutic exercise program through telerehabilitation tools in COVID-19 patients with mild to moderate symptomatology in the acute stage. Materials and Methods: A total of 40 subjects were randomized an experimental group, based on muscle conditioning, and in a control group, who did not perform physical activity. Thirty-six subjects, 18 in each group, completed the one-week intervention. We measured the six-minute walking test, multidimensional dyspnoea-12, thirty seconds sit-to-stand test, and Borg Scale. Results: Both groups were comparable at baseline. Statistically significant improvement between groups (p < 0.05) in favor of the experimental group was obtained. No differences between gender were found (p > 0.05). Ninety percent adherence was found in our program. Conclusion: A one-week telerehabilitation program based on muscle toning exercise is effective, safe, and feasible in COVID-19 patients with mild to moderate symptomatology in the acute stage.
The Large Right Heart Is Associated with the Prolongation of the Procedure Time of Leadless Pacemaker Implantation
Authors: Kataoka Naoya , Imamura Teruhiko , Koi Takahisa , Ueno Hiroshi , Kinugawa Koichiro
Published: July 2021

Background and objectives: Leadless pacemakers are less invasive but are as effective as conventional pacemakers and are increasingly implanted in elderly patients. However, the implantation procedure is sometimes challenging in patients with abnormal anatomy, particularly those with an enlarged right heart. We aimed to determine the right heart parameters that were associated with longer procedure times for leadless pacemaker implantation. Materials and Methods: Among 19 consecutive patients in whom Micra leadless pacemakers (Micra TPS, Medtronic, Minneapolis, MN) were implanted, the diameter and area of both the right atrium and right ventricle were measured by transthoracic echocardiography before the procedure. The right heart parameters that were associated with a procedure time > 60 min were investigated. Results: In the 19 patients (median 81 years old, 10 male) who underwent implantation of the Micra system, 6 (32%) required a procedure time > 60 min. Among the baseline right heart echocardiographic parameters, right atrial diameter and area were significantly associated with a procedure time > 60 min (odds ratio 11.3, 95% confidence interval 1.09–1.17, p = 0.042; and odds ratio 1.57, 95% confidence interval 1.05–2.34, p = 0.029, respectively) at a cutoff of 4.0 cm and 17.0 cm2, respectively. Conclusions: Patients with an enlarged right atrium may not be good candidates for leadless pacemakers given the longer procedure time, and conventional pacemakers should perhaps be recommended as an alternative.
A Combination of GM-CSF and Released Factors from Gamma-Irradiated Tumor Cells Enhances the Differentiation of Macrophages from Bone Marrow Cells and Their Antigen-Presenting Function and Polarization to Type 1
Authors: Chen Lichao , Imamichi Shoji , Tong Ying , Sasaki Yuka , Onodera Takae , Nakamura Satoshi , Igaki Hiroshi , Itami Jun , Masutani Mitsuko
Published: July 2021

Granulocyte-macrophage colony-stimulating factor (GM-CSF) promotes dendritic cell differentiation from precursors, and consequently, enhances the antigen presentation process and adaptive immune responses. With such functions, GM-CSF has been used as immunotherapy in combination with radiotherapy for cancer treatment to augment the survival and activity of immune cells. However, an immune-suppressive tumor microenvironment may cause anergy of T cells. It has also been reported that GM-CSF contributes to the development of myeloid-derived suppressor cells from the precursors. In this study, to analyze the combined effect of GM-CSF and released factors from cancer cells after gamma-ray irradiation on bone marrow cell differentiation and dynamics, we established an in vitro culture system using mouse bone marrow cells, GM-CSF, and conditioned medium from gamma ray irradiated mouse melanoma B16 cells at 24 Gy. We analyzed the gene expression changes of the bone marrow-derived cells on day 6. The results showed that GM-CSF dose-dependently enhanced the differentiation of macrophages from bone marrow cells, their antigen-presenting function and polarization to type I. The results implied the induced macrophages from the bone marrow may potentially contribute to tumor immune responses in a systemic manner when GM-CSF is boosted during photon-beam radiation therapy.
Jominy End Quench Test of Martensitic Stainless Steel X30Cr13
Authors: Landgraf Pierre , Birnbaum Peter , Meza-García Enrique , Grund Thomas , Kräusel Verena , Lampke Thomas
Published: July 2021

In this study, the influence of thermal treatments on the properties of the martensitic stainless steel X30Cr13 (EN 10088-3: 1.4028) were investigated. These steels are characterized by a high hardness as well as corrosion resistance and can be specifically adjusted by heat treatment. In particular, the austenitizing temperature ϑA and cooling rate T˙ affect the hardness and corrosion properties of martensitic stainless steels. In order to investigate these influences, the Jominy end quench tests were performed at varying austenitizing temperatures. The aim is to determine the hardness and corrosion properties as a function of the austenitizing temperature and the cooling rate. The austenitizing temperature strongly influences the solubility of alloying elements within the austenitic lattice as well as the grain size, and thus affects both precipitation and phase transformation kinetics. In consequence, different austenitizing temperatures lead to different macroscopic material properties, like hardness and pitting corrosion potential. The heat treatment was simulated using finite element (FE) method and compared with time-temperature sequences measured at different locations of the Jominy end quench sample using thermocouples. That allows determining the cooling rate T˙ between 800 ∘C and 500 ∘C and to assign it to each location of the Jominy end quench sample. The numerical estimations were in close conformity with the experimental values. By assigning the hardness and pitting corrosion potentials to the respective cooling rates as a function of the austenitizing temperature, it is possible to determine optimum process windows for the required properties.
Reaping the Benefits of Microorganisms in Cropping Systems: Is the Regulatory Policy Adequate?
Authors: Sundh Ingvar , Del Giudice Teresa , Cembalo Luigi
Published: July 2021

Within food plant cropping systems, microorganisms provide vital functions and ecosystem services, such as biological pest and disease control, promotion of plant growth and crop quality, and biodegradation of organic matter and pollutants. The beneficial effects of microorganisms can be achieved and/or enhanced by agricultural management measures that target the resident microbial biodiversity or by augmentation with domesticated and propagated microbial strains. This study presents a critical review of the current legislation and regulatory policies pertaining to the utilization of plant-beneficial microorganisms in the European Union (EU). For augmentative approaches, the nature of the intended effect and the product claim determine how a microbiological product is categorized and regulated, and pre-market authorization may be mandatory. Typically, microbial products have been incorporated into frameworks that were designed for evaluating non-living substances, and are therefore not well suited to the specific properties of live microorganisms. We suggest that regulatory harmonization across the sector could stimulate technical development and facilitate implementation of crop management methods employing microorganisms. Possible scenarios for regulatory reform in the longer term are discussed, but more investigation into their feasibility is needed. The findings of this study should serve as a catalyst for more efficient future use of plant-beneficial microorganisms, to the benefit of agriculture as well as the environment.
The Isolation of Aeromonas Species and Other Common Enteric Bacterial Pathogens from Patients with Gastroenteritis in an Australian Population
Authors: Yuwono Christopher , Wehrhahn Michael C. , Liu Fang , Riordan Stephen M. , Zhang Li
Published: July 2021

Aeromonas species are emerging human enteric pathogens. However, systematic analysis of Aeromonas species infection in human gastroenteritis in comparison with other enteric bacterial pathogens in the Australian population is lacking. Here we analysed the isolation of Aeromonas species and other bacterial pathogens in five consecutive years (2015–2019) from 375,842 stool samples of patients with gastroenteritis in a large Australian diagnostic laboratory and identified a subset (48 isolates) of Aeromonas isolates to species level, using multilocus phylogenetic analysis. Aeromonas species were the third most common bacterial pathogens, following Campylobacter and Salmonella species. Aeromonas infection rate was significantly correlated with increasing age (p < 0.001). Aeromonas species were more often isolated in warm seasons and in males than females (p < 0.001). Five Aeromonas species were identified. Most of the infections were from three species, namely Aeromonas veronii (52%), Aeromonas caviae (27%) and Aeromonas hydrophila (12.5%). The majority of patients with Aeromonas species infection did not have a documented overseas travel history. The findings from this study support the importance of Aeromonas species in human gastroenteritis and suggest that the sources of Aeromonas infection in Australian patients should be further investigated.
Rheological Properties of Ultra-Fine Tailings Cemented Paste Backfill under Ultrasonic Wave Action
Authors: Ren Weicheng , Gao Rugao , Zhang Youzhi , Hou Maoxin
Published: July 2021

Ultra-fine tailings cemented paste backfill (UCPB) exhibits special rheological characteristics with the effect of an ultrasonic sound field. In this study, in order to explore the thickening effect of slurry under ultrasonic wave action, we examined the rheological properties with ultrasonic wave tests of UCPB and the rheological properties after ultrasonic wave tests of UCPB. We found that the rheological curve of the slurry changed; the Herschel–Bulkley (HB) model in the initial state transformed into the Bingham model under the action of ultrasound. Ultrasonic waves have a positive effect on reducing slurry viscosity and yield stress. The rheological test of the slurry with ultrasonic wave action had a positive effect on significantly reducing the apparent viscosity and initial yield stress of slurry with a 62% mass concentration. The rheological test of slurry with ultrasonic wave action and the rheological test after ultrasonic wave action both have positive effects on reducing the viscosity and yield stress of the slurry with a 64% to 68% mass concentration; the overall effect of reducing the viscosity and yield stress of UCPB is greater after ultrasonic wave action of UCPB.
Laboratory Investigations Coupled to VIR/Dawn Observations to Quantify the Large Concentrations of Organic Matter on Ceres
Authors: Vinogradoff Vassilissa , Poggiali Giovanni , Raponi Andrea , Ciarniello Mauro , De Angelis Simone , Ferrari Marco , Castillo-Rogez Julie C , Brucato John , De Sanctis Maria-Cristina
Published: July 2021

Organic matter directly observed at the surface of an inner planetary body is quite infrequent due to the usual low abundance of such matter and the limitation of the infrared technique. Fortuitously, the Dawn mission has revealed, thanks to the Visible and InfraRed mapping spectrometer (VIR), large areas rich in organic matter at the surface of Ceres, near Ernutet crater. The origin of the organic matter and its abundance in association with minerals, as indicated by the low altitude VIR data, remains unclear, but multiple lines of evidence support an endogenous origin. Here, we report an experimental investigation to determine the abundance of the aliphatic carbon signature observed on Ceres. We produced relevant analogues containing ammoniated-phyllosilicates, carbonates, aliphatic carbons (coals), and magnetite or amorphous carbon as darkening agents, and measured their reflectance by infrared spectroscopy. Measurements of these organic-rich analogues were directly compared to the VIR spectra taken from different locations around Ernutet crater. We found that the absolute reflectance of our analogues is at least two orders of magnitude higher than Ceres, but the depths of absorption bands match nicely the ones of the organic-rich Ceres spectra. The choices of the different components are discussed in comparison with VIR data. Relative abundances of the components are extrapolated from the spectra and mixture composition, considering that the differences in reflectance level is mainly due to optical effects. Absorption bands of Ceres’ organic-rich spectra are best reproduced by around 20 wt.% of carbon (a third being aliphatic carbons), in association with around 20 wt.% of carbonates, 15 wt.% of ammoniated-phyllosilicate, 20 wt.% of Mg-phyllosilicates, and 25 wt.% of darkening agent. Results also highlight the pertinence to use laboratory analogues in addition to models for planetary surface characterization. Such large quantities of organic materials near Ernutet crater, in addition to the amorphous carbon suspected on a global scale, requires a concentration mechanism whose nature is still unknown but that could potentially be relevant to other large volatile-rich bodies.
Diffraction Features from (101¯4) Calcite Twins Mimicking Crystallographic Ordering
Authors: Németh Péter
Published: July 2021

During phase transitions the ordering of cations and/or anions along specific crystallographic directions can take place. As a result, extra reflections may occur in diffraction patterns, which can indicate cell doubling and the reduction of the crystallographic symmetry. However, similar features may also arise from twinning. Here the nanostructures of a glendonite, a calcite (CaCO3) pseudomorph after ikaite (CaCO3·6H2O), from Victoria Cave (Russia) were studied using transmission electron microscopy (TEM). This paper demonstrates the occurrence of extra reflections at positions halfway between the Bragg reflections of calcite in 0kl electron diffraction patterns and the doubling of d104 spacings (corresponding to 2∙3.03 Å) in high-resolution TEM images. Interestingly, these diffraction features match with the so-called carbonate c-type reflections, which are associated with Mg and Ca ordering, a phenomenon that cannot occur in pure calcite. TEM and crystallographic analysis suggests that, in fact, (101¯4) calcite twins and the orientation change of CO3 groups across the twin interface are responsible for the extra reflections.
Lacustrine Slope-Related Soft-Sediment Deformation Structures in the Cretaceous Gyeokpori Formation, Buan Area, SW Korea, and Volcanism-Induced Seismic Shocks as Their Possible Trigger
Authors: Byun Uk Hwan , van Loon A.J. (Tom) , Ko Kyoungtae
Published: July 2021

The Gyeokpori Formation in the Buan volcanic area primarily contains siliciclastic rocks interbedded with volcanoclastics. These sediments are characterized by a variety of soft-sediment deformation structures (SSDS). The SSDS in the Gyeokpori Formation are embedded in poorly sorted conglomerates; slump folds are also present in the formation. The deformation mechanisms and triggers causing the deformation are not yet clear. In the present study, the trigger of the SSDS in the Gyeokpori Formation was investigated using facies analysis. This included evaluation of the reworking process of both cohesive and non-cohesive sediments. The analysis indicates that the SSDS are directly or indirectly associated with the alternation of conglomerates and mud layers with clasts. These layers underwent non-cohesive and cohesive deformation, respectively, which promoted SSDS formation. The slump folds were controlled by the extent of cohesive and non-cohesive deformation experienced by the sediment layers in the slope environment. The SSDS deformation style and morphology differ, particularly in the case of reworking by slump activity. This study contributes to the understanding of lacustrine slope-related soft-sediment deformation structures.
In Vitro Reconstitution of Yeast Translation System Capable of Synthesizing Long Polypeptide and Recapitulating Programmed Ribosome Stalling
Authors: Nagai Riku , Xu Yichen , Liu Chang , Shimabukuro Ayaka , Takeuchi-Tomita Nono
Published: July 2021

The rates of translation elongation or termination in eukaryotes are modulated through cooperative molecular interactions involving mRNA, the ribosome, aminoacyl- and nascent polypeptidyl-tRNAs, and translation factors. To investigate the molecular mechanisms underlying these processes, we developed an in vitro translation system from yeast, reconstituted with purified translation elongation and termination factors, utilizing CrPV IGR IRES-containing mRNA, which functions in the absence of initiation factors. The system is capable of synthesizing not only short oligopeptides but also long reporter proteins such as nanoluciferase. By setting appropriate translation reaction conditions, such as the Mg2+/polyamine concentration, the arrest of translation elongation by known ribosome-stalling sequences (e.g., polyproline and CGA codon repeats) is properly recapitulated in this system. We describe protocols for the preparation of the system components, manipulation of the system, and detection of the translation products. We also mention critical parameters for setting up the translation reaction conditions. This reconstituted translation system not only facilitates biochemical analyses of translation but is also useful for various applications, such as structural and functional studies with the aim of designing drugs that act on eukaryotic ribosomes, and the development of systems for producing novel functional proteins by incorporating unnatural amino acids by eukaryotic ribosomes.
Exogenous Applications of Bio-fabricated Silver Nanoparticles to Improve Biochemical, Antioxidant, Fatty Acid and Secondary Metabolite Contents of Sunflower
Authors: Batool Syeda Umber , Javed Bilal , Sohail  , Zehra Syeda Sadaf , Mashwani Zia-ur-Rehman , Raja Naveed Iqbal , Khan Tariq , ALHaithloul Haifa Abdulaziz Sakit , Alghanem Suliman Mohammed , Al-Mushhin Amina A. M. , Hashem Mohamed , Alamri Saad
Published: July 2021

The present study involved the bio-fabrication of silver nanoparticles (AgNPs) by using the Euphorbia helioscopia L. leaves aqueous extract to improve the production of secondary metabolites in industrially important sunflower (Helianthus annuus L.) plants. Phyto-fabrication of AgNPs was confirmed by using spectrophotometry, SEM imaging and X-ray diffraction analysis. The morphological and optical characterization manifested that the AgNPs are crystalline and exist in the size range of 30–100 nm. Various concentrations (10, 20, 40, 60, 80 and 100 mg/L) of AgNPs were applied in combinations on sunflower seeds and crop plants. The effects of biosynthesized AgNPs were evaluated for agro-morphological parameters (plant height, flowering initiation and seed weight), biochemical metabolites (chlorophyll, proline, soluble sugar, amino acid and protein contents) and enzymatic activities (superoxide dismutase and ascorbate peroxidase) in sunflower and 60 mg/L concentration of AgNPs on sunflower seeds and foliar sprays on plants in combination were found to be effective to elicit biochemical modifications to improve secondary metabolites. It was also observed experimentally that 60 mg/L concentration of AgNPs improved the biochemical, fatty acid and enzymatic attributes of sunflower plants, which in turn improved the plant agro-morphological parameters. Near-infrared spectroscopic analysis results confirmed the improvement in the seed quality, oil contents and fatty acid composition (palmitic acid, oleic acid and linoleic acid) after the applications of AgNPs. The findings of the present investigation confirm the exogenous applications of bio-fabricated AgNPs in combinations on seeds and plants to improve the plant yield, seed quality and secondary metabolite contents of the sunflower plants.
Creatine Enhances the Effects of Cluster-Set Resistance Training on Lower-Limb Body Composition and Strength in Resistance-Trained Men: A Pilot Study
Authors: Bonilla Diego A. , Kreider Richard B. , Petro Jorge L. , Romance Ramón , García-Sillero Manuel , Benítez-Porres Javier , Vargas-Molina Salvador
Published: July 2021

Creatine monohydrate (CrM) supplementation has been shown to improve body composition and muscle strength when combined with resistance training (RT); however, no study has evaluated the combination of this nutritional strategy with cluster-set resistance training (CS-RT). The purpose of this pilot study was to evaluate the effects of CrM supplementation during a high-protein diet and a CS-RT program on lower-limb fat-free mass (LL-FFM) and muscular strength. Twenty-three resistance-trained men (>2 years of training experience, 26.6 ± 8.1 years, 176.3 ± 6.8 cm, 75.6 ± 8.9 kg) participated in this study. Subjects were randomly allocated to a CS-RT+CrM (n = 8), a CS-RT (n = 8), or a control group (n = 7). The CS-RT+CrM group followed a CrM supplementation protocol with 0.1 g·kg−1·day−1 over eight weeks. Two sessions per week of lower-limb CS-RT were performed. LL-FFM corrected for fat-free adipose tissue (dual-energy X-ray absorptiometry) and muscle strength (back squat 1 repetition maximum (SQ-1RM) and countermovement jump (CMJ)) were measured pre- and post-intervention. Significant improvements were found in whole-body fat mass, fat percentage, LL-fat mass, LL-FFM, and SQ-1RM in the CS-RT+CrM and CS-RT groups; however, larger effect sizes were obtained in the CS-RT+CrM group regarding whole body FFM (0.64 versus 0.16), lower-limb FFM (0.62 versus 0.18), and SQ-1RM (1.23 versus 0.75) when compared to the CS-RT group. CMJ showed a significant improvement in the CS-RT+CrM group with no significant changes in CS-RT or control groups. No significant differences were found between groups. Eight weeks of CrM supplementation plus a high-protein diet during a CS-RT program has a higher clinical meaningfulness on lower-limb body composition and strength-related variables in trained males than CS-RT alone. Further research might study the potential health and therapeutic effects of this nutrition and exercise strategy.
Dehulled Adlay Consumption Modulates Blood Pressure in Spontaneously Hypertensive Rats and Overweight and Obese Young Adults
Authors: Yeh Wan-Ju , Ko Jung , Cheng Wei-Yi , Yang Hsin-Yi
Published: July 2021

High blood pressure is a crucial risk factor for many cardiovascular diseases, and a diet rich in whole-grain foods may modulate blood pressure. This study investigated the effects of dehulled adlay consumption on blood pressure in vivo. We initially fed spontaneous hypertensive rats diets without (SHR group) or with 12 or 24% dehulled adlay (SHR + LA and SHR + HA groups), and discovered that it could limit blood pressure increases over a 12-week experimental period. Although we found no significant changes in plasma, heart, and kidney angiotensin-converting enzyme activities, both adlay-consuming groups had lower endothelin-1 and creatinine concentrations than the SHR group; the SHR + HA group also had lower aspartate aminotransferase and uric acid levels than the SHR group did. We later recruited 23 participants with overweight and obesity, and they consumed 60 g of dehulled adlay daily for a six-week experimental period. At the end of the study, we observed a significant decrease in the group’s systolic blood pressure (SBP), and the change in SBP was even more evident in participants with high baseline SBP. In conclusion, our results suggested that daily intake of dehulled adlay had beneficial effects in blood-pressure management. Future studies may further clarify the possible underlying mechanisms for the consuming of dehulled adlay as a beneficial dietary approach for people at risk of hypertension.
A Positive Association of Overactivated Immunity with Metabolic Syndrome Risk and Mitigation of Its Association by a Plant-Based Diet and Physical Activity in a Large Cohort Study
Authors: Park Sunmin , Zhang Ting
Published: July 2021

The association between immunity and metabolic syndrome (MetS) has been studied, but its interaction with lifestyles remains unclear. We studied their association and interactions with lifestyles in 40,768 adults aged over 40 years from a large-scale, hospital-based cohort study collected during 2010–2013. White blood cell counts (WBC) and serum C-reactive protein concentrations (CRP) were used as indexes of immune status. The participants were categorized into four groups by the cutoff points of 6.2 × 109/L WBC(L-WBC) and <0.5 mg/dL CRP(L-CRP): L-WBC+L-CRP(n = 25,604), H-WBC+L-CRP(n = 13,880), L-WBC+H-CRP(n = 464), and H-WBC+H-CRP(n = 820). The participants in the H-WBC+L-CRP were younger and had higher numbers of males than the L-WBC+L-CRP. MetS risk was higher by 1.75- and 1.86-fold in the H-WBC+L-CRP and H-WBC+H-CRP, respectively, than the L-WBC+L-CRP. MetS components, including plasma glucose and triglyceride concentrations, and SBP were elevated in H-WBC+L-CRP and H-WBC+H-CRP compared with L-WBC+L-CR+P. The risk of hyperglycemia and high HbA1c was the highest in the H-WBC+H-CRP among all groups. Areas of WBC counts and serum CRP concentrations were 0.637 and 0.672, respectively, in the receiver operating characteristic curve. Daily intake of energy, carbohydrate, protein, and fat was not significantly different in the groups based on WBC counts and CRP. However, a plant-based diet (PBD), physical activity, and non-smoking were related to lowering WBC counts and CRP, but a Western-style diet was linked to elevating CRP. A high PBD intake and smoking status interacted with immunity to influence MetS risk: a low PBD and current smoking were associated with a higher MetS risk in the H-WBC+H-CRP. In conclusion, overactivated immunity determined by CRP and WBC was associated with MetS risk. Behavior modification with PBD and physical activity might be related to immunity regulation.
Melinacidin-Producing Acrostalagmus luteoalbus, a Major Constituent of Mixed Mycobiota Contaminating Insulation Material in an Outdoor Wall
Authors: Andersson (Aino) Maria A. , Salo Johanna , Mikkola Raimo , Marik Tamás , Kredics László , Kurnitski Jarek , Salonen Heidi
Published: July 2021

Occupants may complain about indoor air quality in closed spaces where the officially approved standard methods for indoor air quality risk assessment fail to reveal the cause of the problem. This study describes a rare genus not previously detected in Finnish buildings, Acrostalagmus, and its species A. luteoalbus as the major constituents of the mixed microbiota in the wet cork liner from an outdoor wall. Representatives of the genus were also present in the settled dust in offices where occupants suffered from symptoms related to the indoor air. One strain, POB8, was identified as A. luteoalbus by ITS sequencing. The strain produced the immunosuppressive and cytotoxic melinacidins II, III, and IV, as evidenced by mass spectrometry analysis. In addition, the classical toxigenic species indicating water damage, mycoparasitic Trichoderma, Aspergillus section Versicolores, Aspergillus section Circumdati, Aspergillus section Nigri, and Chaetomium spp., were detected in the wet outdoor wall and settled dust from the problematic rooms. The offices exhibited no visible signs of microbial growth, and the airborne load of microbial conidia was too low to explain the reported symptoms. In conclusion, we suggest the possible migration of microbial bioactive metabolites from the wet outdoor wall into indoor spaces as a plausible explanation for the reported complaints.
All-in-One Pediatric Parenteral Nutrition Admixtures with an Extended Shelf Life—Insight in Correlations between Composition and Physicochemical Parameters
Authors: Gostyńska Aleksandra , Starkowska Joanna , Sobierajska Paulina , Jelińska Anna , Stawny Maciej
Published: July 2021

The administration of three-in-one parenteral nutrition (PN) admixtures to pediatric patients requires special consideration, specifically concerning quality and physicochemical stability. The introduction of a new parenteral amino acid solution into the market prompted us to evaluate Aminoplasmal Paed-based PN admixtures’ stability. The study aimed to determine the physicochemical parameters of the chosen variations of PN admixtures and search for a correlation between its composition and those parameters. One hundred and sixty-eight variations of PN admixtures intended for patients weighing from 10 to 25 kg and aged from 1 to 12 years and differing in the quantitative composition of electrolytes were selected for the study. The samples were prepared using each of the four intravenous lipid emulsions dedicated to pediatric patients: Intralipid 20%, Clinoleic 20%, Lipidem 20%, and Smoflipid 20%. The stability of the PN admixtures was assessed by visual inspection and determination of pH, osmolality, zeta potential, and hydrodynamic mean droplet diameter (MDD) immediately upon preparation and after seven days of storage at the temperature of 5 ± 1 °C with light protection. Pearson’s correlation was used to quantify the relationships between selected ingredients of the PN admixtures and the physicochemical parameters. The PN admixtures were characterized by pH ranging from 5.91 to 7.04, osmolality ranging from 1238 to 1678 mOsm/kg, and zeta potential ranging from −41.3 to −2.16 mV. The changes in pH and osmolality after seven days of storage did not exceed 0.2 and 4.4%, respectively. The homogeneity of the PN admixtures was confirmed by determining the polydispersity index, which ranged from 0.06 to 0.2. The MDD of the studied formulas ranged from 235 to 395 nm and from 233 to 365 nm immediately upon preparation and after the storage period, respectively. Correlations between selected components of the PN admixtures and some physicochemical parameters were found. All Aminoplasmal Paed 10%-based PN admixtures were characterized by appropriate physicochemical quality to be administered via the central veins, both immediately upon preparation and after seven days of storage at the temperature of 5 ± 1 °C with light protection. The applied electrolyte concentrations ranges and types of lipid emulsions in the selected macronutrient quantitative compositions allowed the PN admixtures to remain stable for seven days within the specified limits.
Biodegradable Alginate Films with ZnO Nanoparticles and Citronella Essential Oil—A Novel Antimicrobial Structure
Authors: Motelica Ludmila , Ficai Denisa , Oprea Ovidiu , Ficai Anton , Trusca Roxana-Doina , Andronescu Ecaterina , Holban Alina Maria
Published: July 2021

The petroleum-based materials could be replaced, at least partially, by biodegradable packaging. Adding antimicrobial activity to the new packaging materials can also help improve the shelf life of food and diminish the spoilage. The objective of this research was to obtain a novel antibacterial packaging, based on alginate as biodegradable polymer. The antibacterial activity was induced to the alginate films by adding various amounts of ZnO nanoparticles loaded with citronella (lemongrass) essential oil (CEO). The obtained films were characterized, and antibacterial activity was tested against two Gram-negative (Escherichia coli and Salmonella Typhi) and two Gram-positive (Bacillus cereus and Staphylococcus aureus) bacterial strains. The results suggest the existence of synergy between antibacterial activities of ZnO and CEO against all tested bacterial strains. The obtained films have a good antibacterial coverage, being efficient against several pathogens, the best results being obtained against Bacillus cereus. In addition, the films presented better UV light barrier properties and lower water vapor permeability (WVP) when compared with a simple alginate film. The preliminary tests indicate that the alginate films with ZnO nanoparticles and CEO can be used to successfully preserve the cheese. Therefore, our research evidences the feasibility of using alginate/ZnO/CEO films as antibacterial packaging for cheese in order to extend its shelf life.
Natural and Historical Heritage of the Lisbon Botanical Gardens: An Integrative Approach with Tree Collections
Authors: Cunha Ana Raquel , Soares Ana Luísa , Brilhante Miguel , Arsénio Pedro , Vasconcelos Teresa , Espírito-Santo Dalila , Duarte Maria Cristina , Romeiras Maria Manuel
Published: July 2021

Botanical gardens have long contributed to plant science and have played a leading role in ex situ conservation, namely of threatened tree species. Focusing on the three botanical gardens of Lisbon (i.e., Botanical Garden of Ajuda—JBA, Lisbon Botanical Garden—JBL, and Tropical Botanical Garden—JBT), this study aims to reveal their natural heritage and to understand the historical motivations for their creation. Our results showed that these gardens contain a total of 2551 tree specimens, corresponding to 462 taxa, within 80 plant families. Of these, 85 taxa are found in the three gardens, and more than half of the taxa are hosted in JBL (334 taxa), whereas 230 and 201 taxa were recorded in JBT and JBA, respectively. The motivations for the creation of each garden are reflected in the different geographic origins of the trees they host in their living collections. The Palearctic species are dominant in JBA and JBL, and Tropical trees prevail in JBT. With more than 250 years of history, these gardens hold an invaluable natural and historical heritage, with their living collections providing valuable sources of information for the conservation of threatened plant species, at local and global scales.