bluuebunny/crossref_metadata_2025 huggingface dataset:
A Comprehensive Integrated Genetic Map of the Complete Karyotype of Solea senegalensis (Kaup 1858)
Authors: Merlo Manuel A. , Portela-Bens Silvia , Rodríguez María E. , García-Angulo Aglaya , Cross Ismael , Arias-Pérez Alberto , García Emilio , Rebordinos Laureana
Published: December 2020

Solea senegalensis aquaculture production has experienced a great increase in the last decade and, consequently, the genome knowledge of the species is gaining attention. In this sense, obtaining a high-density genome mapping of the species could offer clues to the aquaculture improvement in those aspects not resolved so far. In the present article, a review and new processed data have allowed to obtain a high-density BAC-based cytogenetic map of S. senegalensis beside the analysis of the sequences of such BAC clones to achieve integrative data. A total of 93 BAC clones were used to localize the chromosome complement of the species and 588 genes were annotated, thus almost reaching the 2.5% of the S. senegalensis genome sequences. As a result, important data about its genome organization and evolution were obtained, such as the lesser gene density of the large metacentric pair compared with the other metacentric chromosomes, which supports the theory of a sex proto-chromosome pair. In addition, chromosomes with a high number of linked genes that are conserved, even in distant species, were detected. This kind of result widens the knowledge of this species’ chromosome dynamics and evolution.
GC and Repeats Profiling along Chromosomes—The Future of Fish Compositional Cytogenomics
Authors: Matoulek Dominik , Borůvková Veronika , Ocalewicz Konrad , Symonová Radka
Published: December 2020

The study of fish cytogenetics has been impeded by the inability to produce G-bands that could assign chromosomes to their homologous pairs. Thus, the majority of karyotypes published have been estimated based on morphological similarities of chromosomes. The reason why chromosome G-banding does not work in fish remains elusive. However, the recent increase in the number of fish genomes assembled to the chromosome level provides a way to analyse this issue. We have developed a Python tool to visualize and quantify GC percentage (GC%) of both repeats and unique DNA along chromosomes using a non-overlapping sliding window approach. Our tool profiles GC% and simultaneously plots the proportion of repeats (rep%) in a color scale (or vice versa). Hence, it is possible to assess the contribution of repeats to the total GC%. The main differences are the GC% of repeats homogenizing the overall GC% along fish chromosomes and a greater range of GC% scattered along fish chromosomes. This may explain the inability to produce G-banding in fish. We also show an occasional banding pattern along the chromosomes in some fish that probably cannot be detected with traditional qualitative cytogenetic methods.
Recent Development on Plant Aldehyde Dehydrogenase Enzymes and Their Functions in Plant Development and Stress Signaling
Authors: Tola Adesola J. , Jaballi Amal , Germain Hugo , Missihoun Tagnon D.
Published: December 2020

Abiotic and biotic stresses induce the formation of reactive oxygen species (ROS), which subsequently causes the excessive accumulation of aldehydes in cells. Stress-derived aldehydes are commonly designated as reactive electrophile species (RES) as a result of the presence of an electrophilic α, β-unsaturated carbonyl group. Aldehyde dehydrogenases (ALDHs) are NAD(P)+-dependent enzymes that metabolize a wide range of endogenous and exogenous aliphatic and aromatic aldehyde molecules by oxidizing them to their corresponding carboxylic acids. The ALDH enzymes are found in nearly all organisms, and plants contain fourteen ALDH protein families. In this review, we performed a critical analysis of the research reports over the last decade on plant ALDHs. Newly discovered roles for these enzymes in metabolism, signaling and development have been highlighted and discussed. We concluded with suggestions for future investigations to exploit the potential of these enzymes in biotechnology and to improve our current knowledge about these enzymes in gene signaling and plant development.
Mapping the Transcriptional and Fitness Landscapes of a Pathogenic E. coli Strain: The Effects of Organic Acid Stress under Aerobic and Anaerobic Conditions
Authors: Bushell Francesca , Herbert John M. J. , Sannasiddappa Thippeswamy H. , Warren Daniel , Turner A. Keith , Falciani Francesco , Lund Peter A.
Published: December 2020

Several methods are available to probe cellular responses to external stresses at the whole genome level. RNAseq can be used to measure changes in expression of all genes following exposure to stress, but gives no information about the contribution of these genes to an organism’s ability to survive the stress. The relative contribution of each non-essential gene in the genome to the fitness of the organism under stress can be obtained using methods that use sequencing to estimate the frequencies of members of a dense transposon library grown under different conditions, for example by transposon-directed insertion sequencing (TraDIS). These two methods thus probe different aspects of the underlying biology of the organism. We were interested to determine the extent to which the data from these two methods converge on related genes and pathways. To do this, we looked at a combination of biologically meaningful stresses. The human gut contains different organic short-chain fatty acids (SCFAs) produced by fermentation of carbon compounds, and Escherichia coli is exposed to these in its passage through the gut. Their effect is likely to depend on both the ambient pH and the level of oxygen present. We, therefore, generated RNAseq and TraDIS data on a uropathogenic E. coli strain grown at either pH 7 or pH 5.5 in the presence or absence of three SCFAs (acetic, propionic and butyric), either aerobically or anaerobically. Our analysis identifies both known and novel pathways as being likely to be important under these conditions. There is no simple correlation between gene expression and fitness, but we found a significant overlap in KEGG pathways that are predicted to be enriched following analysis of the data from the two methods, and the majority of these showed a fitness signature that would be predicted from the gene expression data, assuming expression to be adaptive. Genes which are not in the E. coli core genome were found to be particularly likely to show a positive correlation between level of expression and contribution to fitness.
Molecular Genetics of Glaucoma: Subtype and Ethnicity Considerations
Authors: Zukerman Ryan , Harris Alon , Verticchio Vercellin Alice , Siesky Brent , Pasquale Louis R. , Ciulla Thomas A.
Published: December 2020

Glaucoma, the world’s leading cause of irreversible blindness, is a complex disease, with differential presentation as well as ethnic and geographic disparities. The multifactorial nature of glaucoma complicates the study of genetics and genetic involvement in the disease process. This review synthesizes the current literature on glaucoma and genetics, as stratified by glaucoma subtype and ethnicity. Primary open-angle glaucoma (POAG) is the most common cause of glaucoma worldwide, with the only treatable risk factor (RF) being the reduction of intraocular pressure (IOP). Genes associated with elevated IOP or POAG risk include: ABCA1, AFAP1, ARHGEF12, ATXN2, CAV1, CDKN2B-AS1, FOXC1, GAS7, GMDS, SIX1/SIX6, TMCO1, and TXNRD2. However, there are variations in RF and genetic factors based on ethnic and geographic differences; it is clear that unified molecular pathways accounting for POAG pathogenesis remain uncertain, although inflammation and senescence likely play an important role. There are similar ethnic and geographic complexities in primary angle closure glaucoma (PACG), but several genes have been associated with this disorder, including MMP9, HGF, HSP70, MFRP, and eNOS. In exfoliation glaucoma (XFG), genes implicated include LOXL1, CACNA1A, POMP, TMEM136, AGPAT1, RBMS3, and SEMA6A. Despite tremendous progress, major gaps remain in resolving the genetic architecture for the various glaucoma subtypes across ancestries. Large scale carefully designed studies are required to advance understanding of genetic loci as RF in glaucoma pathophysiology and to improve diagnosis and treatment options.
Strawberry FaWRKY25 Transcription Factor Negatively Regulated the Resistance of Strawberry Fruits to Botrytis cinerea
Authors: Jia Sizhen , Wang Yuanhua , Zhang Geng , Yan Zhiming , Cai Qingsheng
Published: December 2020

WRKY genes and jasmonic acid (JA) play a crucial role in plants’ responses against biotic and abiotic stress. However, the regulating mechanism of WRKY genes on strawberry fruits’ resistance against Botrytis cinerea is largely unknown, and few studies have been performed on their effect on the JA-mediated defense mechanism against B. cinerea. This study explored the effect of FaWRKY25 on the JA-mediated strawberry resistance against B. cinerea. Results showed that the JA content decreased significantly as the fruits matured, whereas the FaWRKY25 expression rose substantially, which led to heightened susceptibility to B. cinerea and in strawberries. External JA treatment significantly increased the JA content in strawberries and reduced the FaWRKY25 expression, thereby enhancing the fruits’ resistance against B. cinerea. FaWRKY25 overexpression significantly lowered the fruits’ resistance against B. cinerea, whereas FaWRKY25 silencing significantly increased resistance. Moreover, FaWRKY25 overexpression significantly lowered the JA content, whereas FaWRKY25 silencing significantly increased it. FaWRKY25 expression level substantially affects the expression levels of genes related to JA biosynthesis and metabolism, other members of the WRKY family, and defense genes. Accordingly, FaWRKY25 plays a crucial role in regulating strawberries’ resistance against B. cinerea and may negatively regulate their JA-mediated resistance mechanism against B. cinerea.
Extreme Rainfall over Complex Terrain: An Application of the Linear Model of Orographic Precipitation to a Case Study in the Italian Pre-Alps
Authors: Abbate Andrea , Papini Monica , Longoni Laura
Published: December 2020

Intense meteorological events are the primary cause of geohazard phenomena in mountain areas. In this paper, we present a study of the intense rainfall event that occurred in the provinces of Lecco and Sondrio from 11 to 12 June 2019. The aim of our work is to understand the effect of local topography on the spatial distribution of rainfall and to attempt the reconstruction of a realistic rainfall field relative to that extreme event. This task represents a challenge in the context of complex orography. Classical rain-gauge interpolation techniques, such as Kriging, may be too approximate, while meteorological models can be complex and often unable to accurately predict rainfall extremes. For these reasons, we tested the linear upslope model (LUM) designed for estimating rainfall records in orographic precipitation. This model explicitly addresses the dependence of rainfall intensification caused by the terrain elevation. In our case study, the available radio sounding data identified the convective nature of the event with a sustained and moist southern flow directed northward across the Pre-Alps, resulting in an orographic uplift. The simulation was conducted along a smoothed elevation profile of the local orography. The result was a reliable reconstruction of the rainfall field, validated with the ground-based rain gauge data. The error analysis revealed a good performance of the LUM with a realistic description of the interaction between the airflow and local orography. The areas subjected to rainfall extremes were correctly identified, confirming the determinant role of complex terrain in precipitation intensification.
The Past Erased, the Future Stolen: Lignite Extractivism as Germany’s Trope for the Anthropocene
Authors: Braunbeck Helga G.
Published: December 2020

Coal, and even more so, brown coal or lignite, is currently under-researched in the energy humanities. Lignite still provides approximately 25% of “green” Germany’s energy; its extraction obliterates human settlements and vibrant ecosystems, and its incineration produces more CO2 than any other fossil fuel, contributing massively to climate change. After discussing German mining history, the genres of the energy narrative, the bioregional novel, and ecopoetry, and earlier literary treatments of lignite mining, I analyze recent lignite novels by Anja Wedershoven, Andreas Apelt, Bernhard Sinkel, and Ingrid Bachér, and ecopoems by Max Czollek and Marion Poschmann. I discuss socioenvironmental issues such as “slow violence” and “environmental injustice” enacted upon rural communities that are being resettled in “sacrifice zones” for national energy needs; political–economic entanglements, and activism against this complete devastation of the naturalcultural landscape; differences in representation in narrative and lyrical texts; and how the authors frame local perceptions of the mining operations and the resulting “moonscape” within the larger temporal and spatial scales of the Anthropocene. I argue that these literary texts prefigure where the Earth may be headed in the Anthropocene, and that Germany’s lignite extractivism can be considered a trope for the Anthropocene.
The Artificial Intelligence in Digital Pathology and Digital Radiology: Where Are We?
Authors: Giansanti Daniele
Published: December 2020

Thanks to the incredible changes promoted by Information and Communication Technology (ICT) conveyed today by electronic-health (eHealth) and mobile-health (mHealth), many new applications of both organ and cellular diagnostics are now possible [...]
Anti-Aging Physiological Roles of Aryl Hydrocarbon Receptor and Its Dietary Regulators
Authors: Serna Eva , Cespedes Cristina , Vina Jose
Published: December 2020

The vast majority of the literature on the aryl hydrocarbon receptor is concerned with its functions in xenobiotic detoxification. However, in the course of evolution, this receptor had to have physiological (rather than toxicological) functions. Our aim was to review the aryl hydrocarbon receptor’s role in the physiological functions involved in aging. This study was performed by searching the MEDLINE and Google Academic databases. A total of 34 articles were selected that focused specifically on the aryl hydrocarbon receptor and aging, the aryl hydrocarbon receptor and physiological functions, and the combination of both. This receptor’s main physiological functions (mediated by the modulation of gene expression) were cell regeneration, the immune reaction, intestinal homeostasis, and cell proliferation. Furthermore, it was shown that the loss of this receptor led to premature aging. This process may be caused by the dysregulation of hematopoietic stem cells, loss of glucose and lipid homeostasis, increase in inflammation, and deterioration of the brain. We conclude that the aryl hydrocarbon receptor, apart from its well-established role in xenobiotic detoxication, plays an important role in physiological functions and in the aging process. Modulation of the signaling pathway of this receptor could be a therapeutic target of interest in aging.
Leveraging Edge Intelligence for Video Analytics in Smart City Applications
Authors: Rocha Neto Aluizio , Silva Thiago P. , Batista Thais , Delicato Flávia C. , Pires Paulo F. , Lopes Frederico
Published: December 2020

In smart city scenarios, the huge proliferation of monitoring cameras scattered in public spaces has posed many challenges to network and processing infrastructure. A few dozen cameras are enough to saturate the city’s backbone. In addition, most smart city applications require a real-time response from the system in charge of processing such large-scale video streams. Finding a missing person using facial recognition technology is one of these applications that require immediate action on the place where that person is. In this paper, we tackle these challenges presenting a distributed system for video analytics designed to leverage edge computing capabilities. Our approach encompasses architecture, methods, and algorithms for: (i) dividing the burdensome processing of large-scale video streams into various machine learning tasks; and (ii) deploying these tasks as a workflow of data processing in edge devices equipped with hardware accelerators for neural networks. We also propose the reuse of nodes running tasks shared by multiple applications, e.g., facial recognition, thus improving the system’s processing throughput. Simulations showed that, with our algorithm to distribute the workload, the time to process a workflow is about 33% faster than a naive approach.
First Report of Necrophilia in the Form of Necrocoitus among Insects, Involving Two Male Cryptotympana atrata (Fabricius, 1775) Cicadas
Authors: Wang Ji-Shen , Meyer-Rochow Victor Benno
Published: December 2020

The unusual case of a male Cryptotympana atrata cicada from China attempting to mate with a dead male conspecific is described and illustrated. Although hitherto unreported, necrophilic behaviour in the form of an attempted necrocoitus, involving dead male or female corpses, may not be as isolated a case as has been previously assumed, but it does not seem to have been mentioned earlier in the entomological literature. Although the described behaviour could have been an expression of a lack of opportunity to locate a cooperative female, several other possibilities, referred to in the Discussion, exist and should not be dismissed.
Methyl Benzoate Is Superior to Other Natural Fumigants for Controlling the Indian Meal Moth (Plodia interpunctella)
Authors: Mostafiz Md Munir , Hassan Errol , Acharya Rajendra , Shim Jae-Kyoung , Lee Kyeong-Yeoll
Published: December 2020

The Indian meal moth, Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae), is an insect pest that commonly affects stored and postharvest agricultural products. For the control of insect pests and mites, methyl benzoate (MBe) is lethal as a fumigant and also causes contact toxicity; although it has already been established as a food-safe natural product, the fumigation toxicity of MBe has yet to be demonstrated in P. interpunctella. Herein, we evaluated MBe as a potential fumigant for controlling adults of P. interpunctella in two bioassays. Compared to the monoterpenes examined under laboratory conditions, MBe demonstrated high fumigant activity using a 1-L glass bottle at 1 μL/L air within 4 h of exposure. The median lethal concentration (LC50) of MBe was 0.1 μL/L air; the median lethal time (LT50) of MBe at 0.1, 0.3, 0.5, and 1 μL/L air was 3.8, 3.3, 2.8, and 2.0 h, respectively. Compared with commercially available monoterpene compounds used in pest control, MBe showed the highest fumigant toxicity (toxicity order as follows): MBe > citronellal > linalool > 1,8 cineole > limonene. Moreover, in a larger space assay, MBe caused 100% mortality of P. interpunctella at 0.01 μL/cm3 of air after 24 h of exposure. Therefore, MBe can be recommended for use in food security programs as an ecofriendly alternative fumigant. Specifically, it provides another management tool for curtailing the loss of stored food commodities due to P. interpunctella infestation.
Heterotopic Ossification of the Vascular Pedicle after Maxillofacial Reconstructive Surgery Using Fibular Free Flap: Introducing New Classification and Retrospective Analysis
Authors: Knitschke Michael , Siu Kelly , Bäcker Christina , Attia Sameh , Howaldt Hans-Peter , Böttger Sebastian
Published: December 2020

Heterotopic ossification (HO) is one of the described phenomena after maxillofacial reconstructive surgery using fibular free flap (FFF) at the reception-site. The aim of this study was to determine the radiological incidence and form of HO along the fibular vascular pedicle as well as the rate of clinical symptoms if present. CT-scans of 102 patients who underwent jaw reconstructive surgery by using FFF from January 2005 to December 2019 were evaluated concerning the presence of HO. Subsequently, the patient files were evaluated to identify the cases with clinical signs and complications related to the presence of HO. A radiological classification of four different HO types was developed. Out of 102 patients, 29 (28.43%) presented radiological findings of HO. Clinical symptoms were recorded in 10 cases (9.8%) (dysphagia (n = 5), trismus (n = 3), bony masses (n = 2)) and from these only five (4.9%) needed surgical removal of calcified structures. HO occurs significantly in younger patients (mean 52.3 year). In maxillary reconstructions, HO was radiologically visible six months earlier than after mandibular reconstruction. Furthermore, HO is observed after every third maxilla and every fourth mandible reconstruction. This study developed for the first time a classification of four distinct HO patterns. HO types 1 and 2 were mostly observed after mandible reconstruction and type 4 predominantly after maxilla reconstruction.
Should We Reconsider the Necessity of a Refinement of Prostate Cancer Risk Classification and Radiotherapy Treatment Strategy? Experiences from a Retrospective Analysis of Data from a Single Institution
Authors: Temesfői Viktória , Herczeg Róbert , Lőcsei Zoltán , Sebestyén Klára , Sebestyén Zsolt , Mangel László , Damásdi Miklós
Published: December 2020

Background: Radiation therapy has undergone significant technical development in the past decade. However, the complex therapy of intermediate-risk patients with organ-confined prostate carcinoma still poses many questions. Our retrospective study investigated the impact of selected components of the treatment process including radiotherapy, hormone deprivation, risk classification, and patients’ response to therapy. Methods: The impact of delivered dose, planning accuracy, duration of hormone deprivation, risk classification, and the time to reach prostate-specific antigen (PSA) nadir state were analyzed among ninety-nine individuals afflicted with organ-confined disease. Progression was defined as a radiological or biochemical relapse within five years from radiotherapy treatment. Results: We found that 58.3% of the progressive population consisted of intermediate-risk patients. The progression rate in the intermediate group was higher (21.9%) than in the high-risk population (12.1%). Dividing the intermediate group, according to the International Society of Urological Pathology (ISUP) recommendations, resulted in the non-favorable subgroup having the highest rate of progression (33.3%) and depicting the lowest percentage of progression-free survival (66.7%). Conclusion: Extended pelvic irradiation on the regional lymph nodes may be necessary for the ISUP Grade 3 subgroup, similarly to the high-risk treatment. Therapy optimization regarding the intermediate-risk population based on the ISUP subgrouping suggestions is highly recommended in the treatment of organ-confined prostate cancer.
Axial Length and Prevalence of Myopia among Schoolchildren in the Equatorial Region of Brazil
Authors: Yotsukura Erisa , Torii Hidemasa , Ozawa Hiroko , Hida Richard Yudi , Shiraishi Tetsuro , Corso Teixeira Ivan , Rautha Yessa Vervloet Bertollo Lamego , Moraes do Nascimento Caio Felipe , Mori Kiwako , Uchino Miki , Kurihara Toshihide , Negishi Kazuno , Tsubota Kazuo
Published: December 2020

The prevalence of myopia is increasing globally, and the outdoor light environment is considered as a possible factor that can retard myopia. The aim of this study was to evaluate the prevalence of myopia and the light environment in Aracati, equatorial Brazil. We surveyed 421 children (421 right eyes; mean age, 10.6 years) and performed ocular examinations that included non-cycloplegic refraction and axial length (AL). Multiple regression analyses were performed to identify factors affecting myopia such as time spent outdoors and in near work. We measured illuminance and violet light irradiance in Aracati. The mean spherical equivalent (SE) and AL were −0.44 ± 1.38 diopters (D) and 22.98 ± 0.87 mm, respectively. The prevalence of myopia (SE ≤ −0.75 D) and high myopia (SE ≤ −6.0 D/AL ≥ 26.0 mm) was 20.4 and 1.4/0.48%, respectively. Multiple regression analyses showed that myopia was not associated with lifestyle factors. The average illuminance in Aracati was about 100,000 lux from morning to evening. The current results reflect the ALs and the prevalence of myopia among Brazilian schoolchildren. There is a possibility that the light environment in addition to other confounding factors including racial differences affects the ALs and refractive errors.
Hyperandrogenism by Liquid Chromatography Tandem Mass Spectrometry in PCOS: Focus on Testosterone and Androstenedione
Authors: Grassi Giorgia , Polledri Elisa , Fustinoni Silvia , Chiodini Iacopo , Ceriotti Ferruccio , D’Agostino Simona , Filippi Francesca , Somigliana Edgardo , Mantovani Giovanna , Arosio Maura , Morelli Valentina
Published: December 2020

The identification of hyperandrogenism in polycystic ovary syndrome (PCOS) is concerning because of the poor accuracy of the androgen immunoassays (IA) and controversies regarding which androgens should be measured. The aim of our study was to evaluate the impact of the assessment of testosterone (T) and androstenedione (A) by liquid chromatography in tandem with mass spectrometry (LC/MS-MS), in the diagnosis of PCOS. We evaluated 131 patients referred for suspected PCOS. Fourteen patients in total were excluded, some because of other diagnosis (n = 7) or incomplete diagnostic workup (n = 7). We measured T and A both by IA and LC-MS/MS in the 117 subjects included. We calculated free T (fT) by the Vermeulen formula and recorded clinical and metabolic data. 73 healthy females served as controls to derive immunoassays (IA) and LC-MS/MS reference intervals for T, fT and A. PCOS was confirmed in 90 subjects by IA and in 93 (+3.3%) by LC-MS/MS. The prevalence of biochemical hyperandrogenism in PCOS by LC-MS/MS increased from 81.7% to 89.2% if A was also considered. The most frequently elevated androgens were fT (73.1%) and A (64.5%) and they had similar levels of accuracy in differentiating PCOS and controls (0.34 ng/dL, Sn 91% Sp 89%; 1.16 ng/mL, Sn 91% Sp 88%, respectively). Free testosterone correlated with body mass index (BMI), homeostatic model assessment (HOMA)-index, glycated hemoglobin (HbA1c), and sex-binding globulin (SHBG). The results confirm that LC-MS/MS is slightly more sensitive than IA in the diagnosis of PCOS with LC-MS/MS detecting higher levels of fT and A. Moreover, assessment of fT and A by LC-MS/MS had a similar level of accuracy in discriminating between PCOs and control subjects. Lastly, fT by LC-MS/MS correlates with adverse metabolic parameters.
Fenofibrate Reduces the Severity of Neuroretinopathy in a Type 2 Model of Diabetes without Inducing Peroxisome Proliferator-Activated Receptor Alpha-Dependent Retinal Gene Expression
Authors: Enright Jennifer M. , Zhang Sheng , Thebeau Christina , Siebert Emily , Jin Alexander , Gadiraju Veda , Zhang Xiaodong , Chen Shiming , Semenkovich Clay F. , Rajagopal Rithwick
Published: December 2020

Fenofibrate slows the progression of clinical diabetic retinopathy (DR), but its mechanism of action in the retina remains unclear. Fenofibrate is a known agonist of peroxisome proliferator-activated receptor alpha (PPARα), a transcription factor critical for regulating metabolism, inflammation and oxidative stress. Using a DR mouse model, db/db, we tested the hypothesis that fenofibrate slows early DR progression by activating PPARα in the retina. Relative to healthy littermates, six-month-old db/db mice exhibited elevated serum triglycerides and cholesterol, retinal gliosis, and electroretinography (ERG) changes including reduced b-wave amplitudes and delayed oscillatory potentials. These pathologic changes in the retina were improved by oral fenofibrate. However, fenofibrate did not induce PPARα target gene expression in whole retina or isolated Müller glia. The capacity of the retina to respond to PPARα was further tested by delivering the PPARα agonist GW590735 to the intraperitoneal or intravitreous space in mice carrying the peroxisome proliferator response element (PPRE)-luciferase reporter. We observed strong induction of the reporter in the liver, but no induction in the retina. In summary, fenofibrate treatment of db/db mice prevents the development of early DR but is not associated with induction of PPARα in the retina.
Analysis of Quality of Life and Nutritional Status in Elderly Patients with Dysphagia in Order to Prevent Hospital Admissions in a COVID-19 Pandemic
Authors: Fernández-Ruiz Virginia E. , Paredes-Ibáñez Rocío , Armero-Barranco David , Sánchez-Romera Juan Francisco , Ferrer Mercedes
Published: December 2020

(1) Background: Oropharyngeal dysphagia (OD) is currently recognized as one of the geriatric syndromes due to its high frequency in older people and its associated complications, which have a direct impact on quality of life. The main objective is to determine the effectiveness of telehealth consultation for the re-evaluation of nutritional status and quality of life assessment in older people diagnosed with OD associated with active use of thickeners to prevent hospital admissions in a COVID-19 pandemic. (2) Methods: an observational, descriptive, and longitudinal study that included a sample of 33 subjects with age equal or superior to 65 years diagnosed with OD with conserved cognitive capacity. The nutritional status was evaluated through the Mini-Nutritional Assessment (MNA) questionnaire and biochemical parameters and, the quality of life was determined through the Swallowing Quality of Life (SWAL-QOL) questionnaire. (3) Results: Thirty-three older patients with OD were recruited (54.5% women), with a mean age of 83.5 ± 7.6 years. The main cause of OD in the study population was neurodegenerative disease (51.5%), followed by cerebrovascular disease (33.3%), and other causes (15.2%). Sixty point six percent of patients were found to be at risk of malnutrition. The MNA score was significantly correlated to albumin (r: 0.600, p < 0.001) and total proteins (r: 0.435, p = 0.015), but not to total cholesterol (r: −0.116, p = 0.534) or lymphocytes (r: −0.056, p = 0.758). The mean total score of the SWAL-QOL was 75.1 ± 16.4 points. (4) Conclusions: the quality of life of the subjects related to the use of a thickener is good. Although the body mass index (BMI) and average biochemical, nutritional parameters of the subjects are within the range of normality, the MNA has detected a high percentage of subjects with the risk of malnutrition, which suggests the need for continuous re-evaluation in these patients, demonstrating the viability of the telematic route in this research.
Hydroforming of Toroidal Bellows: Process Simulation and Quality Control
Authors: Ye Mengsi , Li Huifang , Wang Yougang , Qian Caifu
Published: December 2020

Having higher capacity to undertake pressures and larger compensation ability compared with the U-shape bellows, toroidal or Ω-shape bellows are being more and more widely used in engineering. The wave-shape and wall thickness reduction of bellows are the most important parameters for measuring the hydroforming quality of the bellows. In order to provide references for actual manufacturing, it is valuable to study the factors influencing the hydroforming process and quality of the bellows. In this paper, finite element simulations of the hydroforming process of a monolayer and single-wave toroidal bellows and a two-layer and four-wave toroidal bellows were carried out. Stress and strain distributions before and after unloading were analyzed and the wave height and wall thickness reduction were examined. The numerical results were verified by the actual hydroforming measurements. In addition, ranges of the significant structural or operating factors for producing better bellows were studied and a formula to compute the wall thickness reduction was fitted based on the sufficient numerical results of the hydroforming simulations.
Analysis of Sn-Bi Solders: X-ray Micro Computed Tomography Imaging and Microstructure Characterization in Relation to Properties and Liquid Phase Healing Potential
Authors: Siroky Georg , Kraker Elke , Rosc Jördis , Kieslinger Dietmar , Brunner Roland , van der Zwaag Sybrand , Kozeschnik Ernst , Ecker Werner
Published: December 2020

This work provides an analysis of X-ray micro computed tomography data of Sn-xBi solders with x = 20, 30, 35, 47, 58 wt.% Bi. The eutectic thickness, fraction of eutectic and primary phase are analyzed. Furthermore, the 3D data is evaluated by means of morphology parameters, such as, shape complexity, flatness, elongation and mean intercept length tensor. The investigated alloys are categorized in three groups based on their morphology, which are described as “complex dominant”, “complex- equiaxed” and “mixed”. The mechanical behavior of Sn-Bi alloys in the semi-solid configuration and the correlation with microstructural parameters are discussed. A varying degree of geometric anisotropy of the investigated alloys is found through the mean intercept length tensor. Representative volume element models for finite element simulations (RVE-FEM) are created from tomography data of each alloy to analyze a correlation of geometric and elastic anisotropy. The simulations reveal an elastic isotropic behavior due to the small difference of elastic constants of primary and eutectic phase. A discussion of properties in the semi-solid state and liquid phase healing is provided.
Correction: Czapik, P. Microstructure and Degradation of Mortar Containing Waste Glass Aggregate as Evaluated by Various Microscopic Techniques. Materials 2020, 13, 2186
Authors: Czapik Przemysław
Published: December 2020

The author wishes to make the following correction to this paper [...]
Laser Powder Bed Fusion of Pure Tungsten: Effects of Process Parameters on Morphology, Densification, Microstructure
Authors: Li Junfeng , Wu Yunxiao , Zhou Bokang , Wei Zhengying
Published: December 2020

Tungsten has been widely used in many industrial fields due to its excellent properties. However, owing to its characteristics of inherent brittleness at room temperature and high melting point, it is difficult to prepare tungsten parts with high complexity via traditional methods. In the present work, tungsten samples were prepared by laser powder bed fusion. The influence of each process parameter including laser power, scanning speed, and hatch spacing on the surface morphology, densification, and microstructure of tungsten samples was systematically investigated. The results showed that the use of the appropriate parameters, especially high laser power, can effectively improve the surface quality and obtain a dense surface. The tungsten samples with a relative density of 98.31% were obtained with optimized parameter combinations: a laser power of 300 W, scanning speed of 400 mm/s, and hatch spacing of 0.08 mm. Compared with scanning speed and hatch spacing, the laser power had a more obvious influence on the relative density. Additionally, for the grain morphology by microstructure inspection, elongated curved grains gradually transformed into fine straight columnar grains as the scanning speed increased. The hatch spacing would change the grain morphology slightly but had no significant effect on the grain size.
Friction Stir Welding of a TRIP Fe49Mn30Cr10Co10C1 High Entropy Alloy
Authors: Shaysultanov Dmitry , Raimov Kazimzhon , Stepanov Nikita , Zherebtsov Sergey
Published: December 2020

The effect of friction stir welding parameters on the structure and properties of Fe49Mn30Cr10Co10C1 high-entropy alloy welds was studied. Due to the development of the TRIP effect, the mechanical behaviour of this alloy was associated with the γ fcc-to-ε hcp martensitic transformation. In the initial condition, the microstructure of the program alloy comprised equiaxed fcc grains and small fractions of the hcp ε-martensite (~5%) and M23C6 carbides (~4%). Friction stir welding of the program alloy resulted in recrystallization of the stir zone and a decrease in the fraction of the carbides to 1–2%; however, the percentage of the hcp phase remained at nearly the same level as that in the initial condition. Post-welding tests showed a considerable increase in the strength and microhardness of the welds both due to the recrystallization-induced decrease in grain size and martensitic transformation.
Cooling under Applied Stress Rejuvenates Amorphous Alloys and Enhances Their Ductility
Authors: Priezjev Nikolai V.
Published: December 2020

The effect of tensile stress applied during cooling of binary glasses on the potential energy states and mechanical properties is investigated using molecular dynamics simulations. We study the three-dimensional binary mixture that was first annealed near the glass transition temperature and then rapidly cooled under tension into the glass phase. It is found that at larger values of applied stress, the liquid glass former freezes under higher strain and its potential energy is enhanced. For a fixed cooling rate, the maximum tensile stress that can be applied during cooling is reduced upon increasing initial temperature above the glass transition point. We also show that the amorphous structure of rejuvenated glasses is characterized by an increase in the number of contacts between smaller type atoms. Furthermore, the results of tensile tests demonstrate that the elastic modulus and the peak value of the stress overshoot are reduced in glasses prepared at larger applied stresses and higher initial temperatures, thus indicating enhanced ductility. These findings might be useful for the development of processing and fabrication methods to improve plasticity of bulk metallic glasses.
Grinding Kinetics Study of Tungsten Ore
Authors: Nava Jennire V. , Coello-Velázquez Alfredo L. , Menéndez-Aguado Juan M.
Published: December 2020

The European Commission (EC) maintains the consideration of tungsten as a critical raw material for the European industry, being the comminution stage of tungsten-bearing minerals an essential step in the tungsten concentration process. Comminution operations involve approximately 3–4% of worldwide energy consumption; therefore, grinding optimization should be a priority. In this study, the grinding behavior of tungsten ore from Barruecopardo Mine (Salamanca, Spain) is analyzed. A protocol based on Austin’s methodology and PBM is developed in order to study the influence of operational and geometallurgical variables on grinding kinetics. In addition to the kinetic parameters, the breakage probability (Si) and breakage function (Bij) is determined. The selection function was formulated for the Barruecopardo Mine with respect to the mill speed.
Predicting the Fatigue Life of an AlSi9Cu3 Porous Alloy Using a Vector-Segmentation Technique for a Geometric Parameterisation of the Macro Pores
Authors: Tomažinčič Dejan , Virk Žiga , Kink Peter Marijan , Jerše Gregor , Klemenc Jernej
Published: December 2020

Most of the published research work related to the fatigue life of porous, high-pressure, die-cast structures is limited to a consideration of individual isolated pores. The focus of this article is on calculating the fatigue life of high-pressure, die-cast, AlSi9Cu3 parts with many clustered macro pores. The core of the presented methodology is a geometric parameterisation of the pores using a vector-segmentation technique. The input for the vector segmentation is a μ-CT scan of the porous material. After the pores are localised, they are parameterised as 3D ellipsoids with the corresponding orientations in the Euclidian space. The extracted ellipsoids together with the outer contour are then used to build a finite-element mesh of the porous structure. The stress–strain distribution is calculated using Abaqus and the fatigue life is predicted using SIMULIA fe-safe. The numerical results are compared to the experimentally determined fatigue lives to prove the applicability of the proposed approach. The outcome of this research is a usable tool for estimating the limiting quantity of a structure’s porosity that still allows for the functional performance and required durability of a product.
Lysophospholipids as Predictive Markers of ST-Elevation Myocardial Infarction (STEMI) and Non-ST-Elevation Myocardial Infarction (NSTEMI)
Authors: Chorell Elin , Olsson Tommy , Jansson Jan-Håkan , Wennberg Patrik
Published: December 2020

The present study explored patterns of circulating metabolites and proteins that can predict future risk for ST-elevation myocardial infarction (STEMI) and non-ST-elevation myocardial infarction (NSTEMI). We conducted a prospective nested case-control study in northern Sweden in individuals who developed STEMI (N = 50) and NSTEMI (N = 50) within 5 years and individually matched controls (N = 100). Fasted plasma samples were subjected to multiplatform mass spectrometry-based metabolomics and multiplex protein analyses. Multivariate analyses were used to elucidate infarction-specific metabolite and protein risk profiles associated with future incident STEMI and NSTEMI. We found that altered lysophosphatidylcholine (LPC) to lysophosphatidylethanolamine (LPE) ratio predicted STEMI and NSTEMI events in different ways. In STEMI, lysophospholipids (mainly LPEs) were lower, whereas in NSTEMI, lysophospholipids (mainly LPEs) were higher. We found a similar response for all detected lysophospholipids but significant alterations only for those containing linoleic acid (C18:2, p < 0.05). Patients with STEMI had higher secretoglobin family 3A member 2 and tartrate-resistant acid phosphate type 5 and lower platelet-derived growth factor subunit A, which are proteins associated with atherosclerosis severity and plaque development mediated via altered phospholipid metabolism. In contrast, patients with NSTEMI had higher levels of proteins associated with inflammation and macrophage activation, including interleukin 6, C-reactive protein, chemerin, and cathepsin X and D. The STEMI risk marker profile includes factors closely related to the development of unstable plaque, including a higher LPC:LPE ratio, whereas NSTEMI is characterized by a lower LPC:LPE ratio and increased inflammation.
Reproducibility of Targeted Lipidome Analyses (Lipidyzer) in Plasma and Erythrocytes over a 6-Week Period
Authors: Loef Marieke , von Hegedus Johannes H. , Ghorasaini Mohan , Kroon Féline P. B. , Giera Martin , Ioan-Facsinay Andreea , Kloppenburg Margreet
Published: December 2020

It is essential to measure lipid biomarkers with a high reproducibility to prevent biased results. We compared the lipid composition and inter-day reproducibility of lipid measurements in plasma and erythrocytes. Samples from 42 individuals (77% women, mean age 65 years, mean body mass index (BMI) 27 kg/m2), obtained non-fasted at baseline and after 6 weeks, were used for quantification of up to 1000 lipid species across 13 lipid classes with the Lipidyzer platform. Intraclass correlation coefficients (ICCs) were calculated to investigate the variability of lipid concentrations between timepoints. The ICC distribution of lipids in plasma and erythrocytes were compared using Wilcoxon tests. After data processing, the analyses included 630 lipids in plasma and 286 in erythrocytes. From these, 230 lipids overlapped between sample types. In plasma, 78% of lipid measurements were reproduced well to excellently, compared to 37% in erythrocytes. The ICC score distribution in plasma (median ICC 0.69) was significantly better than in erythrocytes (median ICC 0.51) (p-value < 0.001). At the class level, reproducibility in plasma was superior for triacylglycerols and cholesteryl esters while ceramides, diacylglycerols, (lyso)phosphatidylethanolamines, and sphingomyelins showed better reproducibility in erythrocytes. Although in plasma overall reproducibility was superior, differences at individual and class levels may favor the use of erythrocytes.
Comprehensive Understanding of Silicon-Nanowire Field-Effect Transistor Impedimetric Readout for Biomolecular Sensing
Authors: Bhattacharjee Abhiroop , Nguyen Thanh Chien , Pachauri Vivek , Ingebrandt Sven , Vu Xuan Thang
Published: December 2020

Impedance sensing with silicon nanowire field-effect transistors (SiNW-FETs) shows considerable potential for label-free detection of biomolecules. With this technique, it might be possible to overcome the Debye-screening limitation, a major problem of the classical potentiometric readout. We employed an electronic circuit model in Simulation Program with Integrated Circuit Emphasis (SPICE) for SiNW-FETs to perform impedimetric measurements through SPICE simulations and quantitatively evaluate influences of various device parameters to the transfer function of the devices. Furthermore, we investigated how biomolecule binding to the surface of SiNW-FETs is influencing the impedance spectra. Based on mathematical analysis and simulation results, we proposed methods that could improve the impedimetric readout of SiNW-FET biosensors and make it more explicable.
Evaluation of a Multi-Isotope Approach as a Complement to Concentration Data within Environmental Forensics
Authors: Pontér Simon , Sutliff-Johansson Stacy , Engström Emma , Widerlund Anders , Mäki Anna , Rodushkina Katerina , Paulukat Cora , Rodushkin Ilia
Published: December 2020

Heavy metal contamination was identified in groundwater monitoring wells surrounding a waste deposit facility at the Rönnskär Cu–Pb–Zn smelter in Skellefteå, Northern Sweden, as well as in brackish water and sediments from the nearby harbor. Following an investigative study of the surrounding area, brackish water from the Baltic Sea and sediments from a nearby harbor were also determined to be contaminated. This study investigated the ranges of isotopic compositions of four elements (Cd, Cu, Pb, and Zn) in smelter materials (ores, products, and waste) and polluted groundwater sediments of the affected area. The study’s objective was to evaluate the variability of the polluting source and identify possible isotope fractionation. This study further assesses the viability of using isotopic information to identify the source of the pollutant. These data were used in combination with multi-element screening analysis and multivariate statistical techniques. Expanding the number of elements utilized in isotope tracing empowers our abilities to decipher the source(s) and the extent of environmental exposure from contamination events related to mining and refining operations.