bluuebunny/crossref_metadata_2025 huggingface dataset:
Research on the effectiveness and strategies of new media in promoting voluntary blood donation from a public health perspective in the post-pandemic era
Authors: Weng Jieqiu , Xu Yongzhu , Xie Chengbing , Tian Yunbo , Wang Fang , Cheng Ying
Published: October 2024

Objective

This study explores the effectiveness of new media in enhancing public enthusiasm for voluntary blood donation in the post-pandemic era and proposes effective publicity strategies to promote the sustainability and healthy development of blood donation activities.

Methods

A questionnaire survey was widely used to collect public opinions and attitudes toward voluntary blood donation. The sample data underwent rigorous reliability and validity analysis to ensure authenticity and reliability. Statistical methods such as correlation analysis and regression analysis were employed to deeply investigate the underlying relationships between factors like new media publicity, emotional value, social recognition, convenience, information reliability, and willingness to donate blood voluntarily. Based on these analyses, a research model was constructed, and relevant hypotheses were verified through empirical methods.

Results

The study found that new media publicity may be associated with increasing the willingness of the public to voluntarily donate blood. Survey participants indicated that they were more likely to consider donating blood after exposure to new media publicity. Among these factors, the emotional value and content conveyed by the publicity appeared to be particularly important. Additionally, our research revealed that social recognition, the convenience of blood donation, and the reliability of promotional information seemed to have a direct impact on the public’s willingness to donate blood. At the same time, these factors may indirectly promote actual blood donation behavior by enhancing the public’s emotional resonance and acceptance of blood donation.

Conclusion

This study suggests that new media may play multiple positive roles in promoting voluntary blood donation. Based on these findings, we propose a series of strategic recommendations, including further optimizing publicity content, striving to enhance the public’s emotional resonance, improving the reliability of promotional information, and enhancing service convenience. These suggestions aim to potentially raise public awareness and willingness to participate in voluntary blood donation, providing a scientific basis and strong support for the promotion of voluntary blood donation in the post-epidemic era.
A greedy assist-as-needed controller for end-effect upper limb rehabilitation robot based on 3-DOF potential field constraints
Authors: Lu Yue , Lin Zixuan , Li Yahui , Lv Jinwang , Zhang Jiaji , Xiao Cong , Liang Ye , Chen Xujiao , Song Tao , Chai Guohong , Zuo Guokun
Published: October 2024

It has been proven that robot-assisted rehabilitation training can effectively promote the recovery of upper-limb motor function in post-stroke patients. Increasing patients’ active participation by providing assist-as-needed (AAN) control strategies is key to the effectiveness of robot-assisted rehabilitation training. In this paper, a greedy assist-as-needed (GAAN) controller based on radial basis function (RBF) network combined with 3 degrees of freedom (3-DOF) potential constraints was proposed to provide AAN interactive forces of an end-effect upper limb rehabilitation robot. The proposed 3-DOF potential fields were adopted to constrain the tangential motions of three kinds of typical target trajectories (one-dimensional (1D) lines, two-dimensional (2D) curves and three-dimensional (3D) spirals) while the GAAN controller was designed to estimate the motor capability of a subject and provide appropriate robot-assisted forces. The co-simulation (Adams-Matlab/Simulink) experiments and behavioral experiments on 10 healthy volunteers were conducted to validate the utility of the GAAN controller. The experimental results demonstrated that the GAAN controller combined with 3-DOF potential field constraints enabled the subjects to actively participate in kinds of tracking tasks while keeping acceptable tracking accuracies. 3D spirals could be better in stimulating subjects’ active participation when compared to 1D and 2D target trajectories. The current GAAN controller has the potential to be applied to existing commercial upper limb rehabilitation robots.
Targeted weed management of Palmer amaranth using robotics and deep learning (YOLOv7)
Authors: Balabantaray Amlan , Behera Shaswati , Liew CheeTown , Chamara Nipuna , Singh Mandeep , Jhala Amit J. , Pitla Santosh
Published: October 2024

Effective weed management is a significant challenge in agronomic crops which necessitates innovative solutions to reduce negative environmental impacts and minimize crop damage. Traditional methods often rely on indiscriminate herbicide application, which lacks precision and sustainability. To address this critical need, this study demonstrated an AI-enabled robotic system, Weeding robot, designed for targeted weed management. Palmer amaranth (Amaranthus palmeri S. Watson) was selected as it is the most troublesome weed in Nebraska. We developed the full stack (vision, hardware, software, robotic platform, and AI model) for precision spraying using YOLOv7, a state-of-the-art object detection deep learning technique. The Weeding robot achieved an average of 60.4% precision and 62% recall in real-time weed identification and spot spraying with the developed gantry-based sprayer system. The Weeding robot successfully identified Palmer amaranth across diverse growth stages in controlled outdoor conditions. This study demonstrates the potential of AI-enabled robotic systems for targeted weed management, offering a more precise and sustainable alternative to traditional herbicide application methods.
A single session of coordinative motor training does not improve spatial ability performances in healthy children
Authors: Morawietz Christina , Wissmann Anna Maria , Kuehne Till , Muehlbauer Thomas
Published: October 2024

Background

In recent years, studies have found small-to-medium positive effects of physical activity on academic achievement. Already acute bouts of exercise appear to improve certain cognitive functions. Spatial abilities are one aspect of cognition that is encountered frequently in daily life and that is closely related to success in science, technology, engineering, and mathematics (STEM)-subjects. However, little is known about the effects of an acute exercise session on spatial abilities. The aim of this study was therefore to evaluate the effect of a single session of coordinative motor training (CMT) on spatial ability performances in healthy children.

Methods

Forty-nine children were assigned to either a single session of CMT (i.e., obstacle course with motor coordinative and spatial elements) (n = 25, 12 females, mean age: 10.7 ± 0.6 years) or a resting control group (n = 24, 12 females, mean age ± SD: 11.4 ± 0.5 years). Spatial abilities were evaluated in both groups using the Paper Folding Test (PFT), Mental Rotation Test (MRT), Water Level Task (WLT), Corsi Block Test (CBT), and Numbered Cones Run (NCR).

Results

A statistical main effect for Test was observed for the majority of outcomes (i.e., all but the MRT). Test × Group interactions did not reach the level of significance.

Conclusion

The results indicate that a single session of CMT does not improve spatial ability performances of healthy children. Future research should evaluate whether repeated longer-term interventions might be more suitable to generate significant improvements in spatial abilities.
Analysis of fungal diversity in the feces of Arborophila rufipectus
Authors: Ma Xiaoping , Li Junshu , Li Zhiguo , Chen Benping , Ling Zhenwen , Feng Shenglin , Zhong Zhijun , Peng Guangneng , Wang Ya , Jiang Yaozhang , Gu Yu
Published: October 2024

Background

Intestinal fungal composition plays a crucial role in modulating host health, and thus is of great significance in the conservation of endangered bird species. However, research on gut fungal composition in birds is limited. Therefore, in this study, we aimed to examine gut fungal community and potential fecal pathogen composition in wild Arborophila rufipectus.

Methods

Fecal samples were collected from the habitats of wild A. rufipectus and Lophura nycthemera (a widely distributed species belonging to the same family as A. rufipectus) in summer and autumn. Thereafter, RNA was collected and the internal transcribed spacer rRNA gene was sequenced via high-throughput sequencing to investigate seasonal variations in intestinal core fungi, microbial fungi, and potential pathogenic fungi.

Results

The gut microbiota of A. rufipectus and L. nycthemera were highly similar and mainly consisted of three phyla, Ascomycota (58.46%), Basidiomycota (28.80%), and Zygomycota (3.56%), which accounted for 90.82% of the fungal community in all the samples. Further, the predominant genera were Ascomycota_unclassified (12.24%), Fungi_unclassified (8.37%), Davidiella (5.18%), Helotiales_unclassified (2.76%), Wickerhamomyces (1.84%), and Pleosporales_unclassified (1.14%), and the potential fecal pathogens identified included Candida, Cryptococcus, Trichosporon, and Malassezia.

Conclusion

Our results provide evidence that the diversity of intestinal fungi in the endangered species, A. rufipectus, is similar to that in the common species, L. nycthemera, and may serve as a basis for monitoring the status of A. rufipectus and for developing conservation measures.
Agri-Preneurial Resilience and Success: The Correlation and Demographic Characteristics of Smallholders in South Africa
Authors: Agholor Isaac Azikiwe , Chowdhury Ataharul , Yusuf Shehu Folaranmi Gbolahan
Published: October 2024

The incentives and subsidies needed to stimulate growth, resilience, and success in agri-preneurial businesses will only be realized through numerous interventions as agri-preneurship contributes significantly to sustainable agricultural development in South Africa. This study provided a novel insight into agri-preneurial resilience and success and evidenced the hypothesis that there is no significant positive correlation between agri-preneurial resilience, farm success, and selected demographic characteristics of smallholders. We surveyed a total of 200 agri-preneurs who were willing and able to participate in this study. This study used a structured questionnaire that was divided into the following sections: (i) demographic information; (ii) agri-preneurial resilience; and (iii) agri-preneurial success. Descriptive statistics and a regression analysis were employed to articulate responses. Four distinct models were employed to ascertain the goodness of fitness and the hypothesis, and assess the relationship between success, resilience, and selected demographic characteristics of agri-preneurs. To determine resilience, the Connor–Davidson Resilience Scale (CD-RISC) with 10 items was used because the CD-RISC justifies the best psychometric characteristics that portrays the levels of resilience amongst agri-preneurs. In measuring success, the scale items were graduated and ranked on a 5-point scale from 1 to 5. The reliability of the scale was also tested, and α = 0.93 was obtained. This study obtained a Cronbach alpha value of 0.96, indicating optimum reliability. Additionally, we ran a factor analysis to certify the reliability of the variable, which gave one factor from the four items. Significant positive correlations were found between gender, age, education, income, household size, diversification, and agri-preneurial resilience and success. This study concluded that most of the selected demographic characteristics were predictors of agri-preneurial resilience and success. However, demographic variables may be influenced by numerous factors given the heterogeneity of agri-preneurs in the study area.
Citizens’ Perception of Digital Public Services: A Case Study among Romanian Citizens
Authors: Popescu Mirona Ana Maria , Barbu Andreea , Moiceanu Georgiana , Costea-Marcu Iustina-Cristina , Militaru Gheorghe , Simion Petronela Cristina
Published: October 2024

Digital transformation has become a topic of interest since public institutions digitalize their processes to improve efficiency, while citizens wish to solve their issues through online platforms. Even if this topic is a priority, the Digital Economy and Society Index reveals that digital public services from UE are under 30% development and implementation. This paper presents the current research made on digital public services and the perspective of Romanian citizens on the adoption of these platforms. The authors conduct bibliometric research to highlight the current progress and concerns. Furthermore, a quantitative analysis is made to determine the perception of Romanian citizens on digital public services and the factors that influence their decision to use them instead of traditional methods. According to the bibliometric analysis, 23 countries showed a raised interest for digital public services after 2020, which was a crucial moment that pushed digitalization among public institutions. Although Romanian citizens are aware of the benefits brought by digital public services, they encounter issues in terms of lack of information and technical support, knowledge, data security, and the complexity of these platforms. Therefore, the government should implement an integrated approach that considers cultural values as well as reliable and accessible digital solutions.
Study of Pollen Traits, Production, and Artificial Pollination Methods in Zea mays L.
Authors: Landoni Michela , Sangiorgio Stefano , Ghidoli Martina , Cassani Elena , Pilu Roberto
Published: October 2024

The optimization of artificial pollination is crucial in breeding programs for allogamous plants. In maize, achieving a balance between the labor-intensive nature of controlled pollinations and the need for large-scale production of hybrid seeds, along with considerations of germinability and pollen production, determines the success of genetic improvement programs. Breeding programs in maize have resulted in a reduction in the number of tassel branches to increase light interception and plant density in production fields. However, despite this genetic improvement, the decreased pollen production per plant has raised critical concerns regarding pollination and subsequent ear filling, especially under adverse environmental conditions. The aim of this work was the analysis of factors that can contribute to increasing the efficiency of controlled pollination in maize. The data obtained showed that pollen diameter, flavonoid, and phenolic acid content do not influence the percentage of germination and therefore the efficiency of pollination. The quantity of pollen is a central factor in ensuring the efficiency of controlled pollinations, and the data obtained by comparing traditional varieties with modern hybrids of maize showed that an increase in pollen production is determined by the increase in branching of the male inflorescence. Furthermore, we propose the use of a “smart” pollination method to make this step in maize breeding programs easier and faster.
How Can Digital Villages Improve Basic Public Services Delivery in Rural Areas? Evidence from 1840 Counties in China
Authors: Mao Zijun , Zhu Xiyue , Zou Qi , Jin Wen
Published: October 2024

Digital transformation is spreading from urban to rural areas, and the construction of digital villages has become a key growth point for rural sustainable development globally. Digital villages improve the level of basic public services delivery in rural areas via the penetration of digital technology. However, few studies have empirically investigated the theoretical mechanisms underlying the impact of digital villages on various aspects of rural basic public services. To address these gaps, this study investigates the impact mechanisms of digital villages on rural basic public services delivery in terms of accessibility, equity, agility, holistic nature and participation. Using 1840 counties in China as the research sample, this study applies the entropy method to extract a composite index of basic public services and performs correlation, regression, and heterogeneity analyses to examine the effects of digital villages on basic public services delivery. Empirical analysis results show that the construction of digital villages is positively associated with the level of basic public services in rural areas. Meanwhile, heterogeneity analysis results confirms that this relationship is mainly observed in eastern counties but not observed in central and western counties. These findings provide a basis for using digital inclusion to improve basic public services delivery in rural areas and achieve balanced development across regions.
Effects of Straw Input on the Yield and Water-Use Efficiency of Spring Maize in Film-Mulched Farmland
Authors: Lou Yisheng , Zhang Xu , Zhang Shiyu , Li Na , Zhao Yidong , Bai Wei , Sun Zhanxiang , Zhang Zhe
Published: October 2024

To provide a theoretical basis for the sustainable application of autumn mulching technology, we examined the effects of straw input on spring maize yield and water-use efficiency in film-mulched farmland. Based on the positioning tests of different mulching methods conducted in 2013, non-mulching (NM), spring mulching (SM), autumn mulching (AM), and autumn mulching combined with the return of straw (AMS) were selected in western Liaoning from 2018 to 2021. Spring maize yield, yield component factors, soil water content, and water-use efficiency under the four treatments were assessed. In each year, the AMS treatment significantly increased the maize yield, which was 48.22%, 9.33%, 30.66%, and 9.92%, and 11.78%, 7.71%, 12.86%, and 4.77% higher than that obtained after the SM and AM treatments, respectively. However, the harvest index was not significantly improved by AMS. AMS treatment significantly improved the precipitation utilization rate in all assessed years. Moreover, the crop water consumption was significantly increased by AMS treatment. Compared with the NM treatment, water-use efficiencies for economic and biological yield were also significantly improved. Thus, autumn mulching combined with straw-returning technology is an effective technical measure for improve spring maize yield and water-use efficiency in semi-arid areas of western Liaoning.
Modeling and Optimization of Maize Yield and Water Use Efficiency under Biochar, Inorganic Fertilizer and Irrigation Using Principal Component Analysis
Authors: Faloye Oluwaseun Temitope , Ajayi Ayodele Ebenezer , Oguntunde Philip Gbenro , Kamchoom Viroon , Fasina Abayomi
Published: October 2024

This study was conducted to predict the grain yield of a maize crop from easy-to-measure growth parameters and select the best treatment combinations of biochar, inorganic fertilizer, and irrigation for the maize grain yield and water use efficiency (WUE) using the Principal Component Analysis (PCA) technique. Two rates of biochar (0 and 20 t ha−1) and fertilizer (0 and 300 kg ha−1) were applied to the soil, with maize crop planted, and subjected to deficit irrigation at 60, 80, and 100% of full irrigation amounts (FIA). Maize growth parameters (number of leaves—NL, leaf area—LA, leaf area index—LAI, and plant height—PH) were measured weekly. The results showed that the developed principal component regression (PCR) from the easy-to-measure growth parameters were strong and moderate in predicting the maize yield and WUE, with coefficient of determination; r2 values of 0.92 and 0.56, respectively. Using the PCA technique, the integration of irrigation with the least amount of water (60% FAI) with biochar (20 t ha−1) and fertilizer (300 kg ha−1) produced the highest ranking on grain yield and water use efficiency. This optimization technique showed that with the adoption of the integrative approach, 40% of irrigation water could be saved for other agricultural purposes
Genome-Wide Identification and Functional Validation of Actin Depolymerizing Factor (ADF) Gene Family in Gossypium hirsutum L.
Authors: Guo Jingxuan , Zeng Qingtao , Liu Ying , Ba Zhaoyuan , Ma Xiongfeng
Published: October 2024

The Actin Depolymerizing Factor (ADF) protein, highly conserved among eukaryotes, is essential for plant growth, development, and stress responses. Cotton, a vital economic crop with applications spanning oilseed, textiles, and military sectors, has seen a limited exploration of its ADF gene family. This research has identified 118 unique ADF sequences across four principal cotton species: Gossypium hirsutum L., Gossypium barbadense Linn, Gossypium raimondii, and Asiatic cotton. The study found that the structural domains and physicochemical properties of these proteins are largely uniform across species. The ADF genes were classified into four subfamilies with a notable expansion in groups III and IV due to tandem and chromosomal duplication events. A thorough analysis revealed a high degree of conservation in gene structure, including exon counts and the lengths of introns and exons, with the majority of genes containing three exons, aligning with the characteristics of the ADF family. RNA-seq analysis uncovered a spectrum of responses by GhADFs to various abiotic stresses with GhADF19 showing the most significant reaction. Virus-induced gene silencing (VIGS) experiments were conducted to assess the role of GhADF19 in plant growth under abiotic stress. The results demonstrated that plants with silenced GhADF19 exhibited significantly slower growth rates and lower dry weights when subjected to cold, salt, and drought stress compared to the control group. This marked reduction in growth and dry weight under stress conditions highlights the potential importance of GhADF19 in stress tolerance mechanisms.
Determining the Benefits of Biomass: Who Wins, and Who Loses?
Authors: Taylor Daniel , Sparks Joanna , Chong Katie , Röder Mirjam
Published: October 2024

Beyond the technical challenge of using biomass to achieve net zero, non-technical factors also impact the likelihood of biomass succeeding in displacing fossil fuel use, such as social, environmental, and economic challenges. The political bioeconomy in the United Kingdom (UK) has supported a small but significant role for biomass within the country’s energy mix, with policy determining who benefits, and who will continue to benefit, from its use. The revised UK Biomass Strategy of 2023 signalled how the government perceives biomass looking forward, and the commitment to a cross-sectoral sustainability framework has the potential to support a redistributive policy that creates new winners in the UK biomass sector. Maximising the redistributive effects of policy is hindered by the siloed nature of policymaking around biomass and undermined by a lack of social legitimacy, both of which must be addressed to enable biomass to contribute towards decoupling the UK’s economy from fossil fuels and to ensure a sustainable transition.
Ginger Phytotoxicity: Potential Efficacy of Extracts, Metabolites and Derivatives for Weed Control
Authors: Zorrilla Jesús G. , Rial Carlos , Martínez-González Miriam I. , Molinillo José M. G. , Macías Francisco A. , Varela Rosa M.
Published: October 2024

The negative implications for weeds encourage the finding of novel sources of phytotoxic agents for sustainable management. While traditional herbicides are effective, especially at large scales, the environmental impact and proliferation of resistant biotypes present major challenges that natural sources could mitigate. In this study, the potential of ginger metabolites as phytotoxic agents has been investigated for the first time. Root extracts, prepared via various extraction techniques, showed phytotoxicity in wheat (Triticum aestivum L. cv. Burgos) coleoptile bioassays at 800–100 ppm, and the most active extract (prepared by sonication with ethyl acetate) was purified by chromatographic methods, yielding seven compounds: five phenolic metabolites with gingerol and shogaol structures, β-sitosterol, and linoleic acid. Some of the major phenolic metabolites, especially [6]-shogaol and [6]-gingerol, exerted phytotoxicity on wheat coleoptiles, Plantago lanceolata and Portulaca oleracea (broadleaf dicotyledon weeds). This promoted the study of a collection of derivatives, revealing that the 5-methoxy, oxime, and acetylated derivatives of [6]-shogaol and [6]-gingerol had interesting phytotoxicities, providing clues for improving the stability of the isolated structures. Ginger roots have been demonstrated to be a promising source of bioactive metabolites for weed control, offering novel materials with potential for the development of agrochemicals based on natural products.
Rhizosheath Formation and Its Role in Plant Adaptation to Abiotic Stress
Authors: Li Ying , Hong Yonghui , Chen Yadi , Zhu Nanyan , Jiang Shuqiu , Yao Zixuan , Zhu Min , Ding Jinfeng , Li Chunyan , Xu Weifeng , Guo Wenshan , Zhu Xinkai , Zhang Jianhua
Published: October 2024

The rhizosheath, the layer of soil tightly attached to the roots, protects plants against abiotic stress and other adverse conditions by providing a bridge from the plant root system to the soil. It reduces the formation of air gaps between the root and soil and facilitates the transportation of water at the root–soil interface. It also serves as a favourable niche for plant-growth-promoting rhizobacteria in the surrounding soil, which facilitate the absorption of soil water and nutrients. This review compares the difference between the rhizosheath and rhizosphere, and summarises the molecular and physiological mechanisms of rhizosheath formation, and identifying the causes of rhizosheath formation/non-formation in plants. We summarise the chemical and physical factors (root hair, soil-related factors, root exudates, and microorganisms) that determine rhizosheath formation, and focus on the important functions of the rhizosheath in plants under abiotic stress, especially in drought stress, phosphorus deficiency, aluminium stress, and salinity stress. Understanding the roles played by the rhizosheath and the mechanisms of its formation provides new perspectives for improving plant stress tolerance in the field, which will mitigate the increasing environmental stress conditions associated with on-going global climate change.
The Effects of Different Grazing Periods on the Functional Traits of Leymus chinensis (Trin.) Tzvelev in a Typical Inner Mongolia Steppe
Authors: Xu Hongbin , Zhang Jiatao , Baoyin Taogetao , Zhang Lei , Yuan Ting
Published: October 2024

Plant functional traits are effective indicators and predictors of environmental change, revealing plants’ ecological countermeasures and adaptability through phenotypic plasticity. We conducted a 6-year grazing experiment on typical temperate grassland to assess the impact of different grazing periods on the plasticity and variability of the functional traits of Leymus chinensis and the relationship between traits and individual plant biomass. Our study included four treatments: CK (enclosure), T1 (grazing in May and July), T2 (grazing in June and August), and T3 (grazing in July and September). The results for 13 functional traits indicated that the T3 treatment showed the smallest reduction in individual plant biomass, plant height, leaf area, stem length, and leaf length, making it the most effective type of grassland management and optimal for the maintenance and restoration of L. chinensis traits. Under T1, T2, and T3, the plasticity of stem weight, total leaf weight, total leaf area, and stem length was higher and crucial for regulating individual plant biomass. The results underscore that the changes and plasticity of dominant species under grazing treatments are key to understanding the relationship between ecosystem function and grassland management. This study provides a theoretical basis and data support for the adaptive utilization and restoration management of typical grassland resources.
Allelochemicals Released from Rice Straw Inhibit Wheat Seed Germination and Seedling Growth
Authors: Li Bo , Wu Wenwen , Shen Wenyuan , Xiong Fei , Wang Kaihua
Published: October 2024

Recently, returning rice straw to soil has become a common problem in wheat production because it causes decreased wheat seedling emergence. Allelopathy is an important factor affecting seed germination. However, the effects of rice straw extracts on wheat seed germination and seedling growth remain unclear. Wheat seeds and seedlings were treated with 30 g L−1 of rice leaf extracts (L1), 60 g L−1 of rice leaf extracts (L2), 30 g L−1 of rice stem extracts (S1), 60 g L−1 of rice stem extracts (S2) and sterile water (CK) to study the allelopathic effects of rice straw extracts on wheat seed germination and seedling growth. The α-amylase and antioxidant enzyme activities in wheat seeds; the agronomic traits, photosynthetic indicators, and nutrient contents of wheat seedlings; and the phenolic acids in rice stem extracts were determined. Common allelochemicals, including 4-hydroxybenzoic acid, hydrocinnamic acid, trans-cinnamic acid, vanillic acid, benzoic acid, protocatechualdehyde, caffeic acid, syringic acid, sinapic acid, and salicylic acid, were detected in rice stem extracts. Low-concentration rice leaf and stem extracts (30 g L−1) had no effect on the germination rate of wheat seeds. High-concentration (60 g L−1) rice stem and leaf extracts decreased the seed germination rate by 11.00% and 12.02%. Rice stem extract (60 g L−1) decreased the α-amylase activity, and gibberellin content of wheat seeds but increased superoxide dismutase, peroxidase, and catalase activities and malondialdehyde content in wheat seeds. Allelochemicals entered the internal tissues of wheat seeds, where they decreased the gibberellin content and α-amylase activity and increased the antioxidant enzyme activity, ultimately leading to an inhibitory effect on seed germination. Rice stem and leaf extracts decreased the SPAD value and photosynthetic indicators of wheat seedlings. Rice stem extract (60 g L−1) decreased the fresh weight and root length of wheat seedlings by 31.37% and 45.46%. Low-concentration rice leaf and stem extract (30 g L−1) had no effect on the nutrient contents of wheat seedlings. Rice leaf and stem extracts (60 g L−1) decreased the nitrogen and potassium contents of wheat seedlings. These results indicated that low-concentration rice leaf and stem extract (30 g L−1) had no effect on wheat seed germination and the high-concentration rice stem extract (60 g L−1) released allelochemicals and inhibited wheat seed germination and seedling growth. These findings provide a basis for the improvement of straw return techniques.
Radiological Evaluation of Vascular Structures in Cats Infected with Immature Worms of Dirofilaria immitis
Authors: Falcón-Cordón Soraya , Falcón-Cordón Yaiza , García-Rodríguez Sara Nieves , Costa-Rodríguez Noelia , Vera-Rodríguez Daniel Julio , Montoya-Alonso José Alberto , Carretón Elena
Published: October 2024

This study aimed to assess thoracic radiographic abnormalities in cats infected with immature stages of Dirofilaria immitis to evaluate the utility of this diagnostic technique during early infection. A total of 123 cats from a hyperendemic area were classified into three groups: asymptomatic cats seronegative to anti-D.-immitis antibodies (Group A), seropositive asymptomatic cats (Group B), and seropositive cats with clinical signs that were at high risk of heartworm-associated respiratory disease (HARD) (Group C). Radiographic measurements and lung parenchymal abnormalities were analyzed and compared across the groups. Significant differences in several parameters, including CrPA/R4, and CdPA/R9 ratios, were observed between healthy and seropositive cats, suggesting early arterial damage even in the absence of adult worms. Other parameters that showed differences between healthy and infected cats were CVC/Ao and CVC/R4 ratios, but not the VHS. Group C exhibited a marked bronchointerstitial pattern, indicating severe parenchymal alterations associated with clinical signs. The study demonstrated that thoracic radiography can detect early vascular and parenchymal changes in feline D. immitis infections, providing valuable information for diagnosing HARD. However, it also highlights the limitations of radiographic techniques, as some seropositive cats displayed no significant abnormalities. The findings underscore the importance of combining radiography with clinical and serological assessments for a more accurate diagnosis of feline heartworm disease.
Machine Learning and Wavelet Transform: A Hybrid Approach to Predicting Ammonia Levels in Poultry Farms
Authors: Küçüktopçu Erdem , Cemek Bilal , Simsek Halis
Published: October 2024

Ammonia (NH3) is a major pollutant in poultry farms, negatively impacting bird health and welfare. High NH3 levels can cause poor weight gain, inefficient feed conversion, reduced viability, and financial losses in the poultry industry. Therefore, accurate estimation of NH3 concentration is crucial for environmental protection and human and animal health. Three widely used machine learning (ML) algorithms—extreme learning machine (ELM), k-nearest neighbor (KNN), and random forest (RF)—were initially used as base algorithms. The wavelet transform (WT) with ten levels of decomposition was then applied as a preprocessing method. Three statistical metrics, including the mean absolute error (MAE) and the correlation coefficient (R), were used to evaluate the predictive accuracies of algorithms. The results indicate that the RF algorithms perform robustly individually and in combination with the WT. The RF-WT algorithm performed best using the air temperature, relative humidity, and air velocity inputs with a MAE of 0.548 ppm and an R of 0.976 for the testing dataset. In summary, applying WT to the inputs significantly improved the predictive power of the ML algorithms, especially for inputs that initially had a low correlation with the NH3 values.
Expression of Genes Related to Meat Productivity, Metabolic and Morphological Significance of Broiler Chickens with the Use of Nutritional Phytochemicals
Authors: Selionova Marina I. , Trukhachev Vladimir I. , Zagarin Artem Yu. , Kulikov Egor I. , Dmitrenko Dmitry M. , Martynova Vera N. , Kravchenko Arina K. , Vertiprakhov Vladimir G.
Published: October 2024

The study aimed to analyze gene expression linked to skeletal muscle growth and lipid metabolism in broiler chickens fed with plant extracts. Five groups of chickens were formed: four experimental groups and one control group. The diets of the experimental groups were supplemented with different plant extracts: chicory, St. John’s wort, maral root, and creeping thyme, whereas the control group received feed without phytobiotic compounds. Weekly weighings were conducted (n = 36). The chickens were slaughtered at day 26 for tissue sampling of four birds from each group. Gene expression (MYOG, MSTN, FASN) related to muscle growth and fatty acid synthesis was analyzed using the β-actin ACTB gene as a reference. Blood samples were taken at day 35 for biochemical analysis and anatomical dissection was performed. The study revealed that using plant extracts from chicory, thyme, and maral root increased MYOG gene activity by 4.21, 7.45, and 8.93 times, respectively. T. serpyllum extract boosted the MSTN gene by 10.93 times, impacting muscle growth regulation. FASN gene expression for fatty acid synthesis increased significantly by 18.22–184.12 times with plant extracts. The best results regarding meat productivity of chickens were obtained when using R. carthamoides extract. The results of the study will serve as a basis for further development of a phytocomposition designed to increase the meat productivity of broiler chickens in the production of environmentally safe poultry products.
Effects of Trace Mineral Source on Growth Performance, Antioxidant Activity, and Meat Quality of Pigs Fed an Oxidized Soy Oil Supplemented Diet
Authors: Zhang Ge , Huang Jingyi , Sun Zhiqiang , Guo Yuhan , Lin Gang , Zhang Zeyu , Zhao Jinbiao
Published: October 2024

This study investigates the effects of oil quality and trace mineral source on the growth performance, antioxidant activity, and meat quality of growing–finishing pigs. A total of 180 crossbred pigs (Duroc × Landrace × Large White [64.4 ± 1.95]) were randomly allocated five dietary treatments based on body weight (BW) and sex in a 30 d trial. Pigs were fed five diets: (i) fresh soy oil + inorganic trace minerals (ITMs) + inorganic selenium (FISI), (ii) oxidized soy oil + ITMs + inorganic selenium (OISI), (iii) fresh soy oil + ITMs + selenium yeast (FISY), (iv) oxidized soy oil + ITMs + selenium yeast (OISY), and (v) oxidized soy oil + organic trace minerals (OTMs) + selenium yeast (OOSY). Each dietary treatment included six replicates and six pigs per replicate (three barrows and three gilts). Feeding OISI resulted in lower average daily gain (ADG) and dressing percentage (p < 0.05). The OOSY group had a higher dressing percentage and activities of serum CAT and GSH-Px in growing–finishing pigs (p < 0.05). In addition, the relative abundance of Campylobacterota in the colonic digesta varied with the quality of soy oil and source of trace minerals (p < 0.05), but no significant differences in short-chain fatty acid concentrations were observed among all dietary groups. In conclusion, adding oxidized soy oil to the diet negatively impacted the ADG and dressing percentage of growing–finishing pigs, and replacing ITMs with OTMs and SY alleviated these negative impacts. A combination of OTMs and SY can support antioxidant capacity to mitigate the negative impacts of oxidized oil on the growth performance and dressing percentage of growing–finishing pigs.
Alpha-Lipoic Acid: Biological Mechanisms and Health Benefits
Authors: Superti Fabiana , Russo Rosario
Published: October 2024

Alpha-lipoic acid (ALA) is a bioactive molecule with significant health effects. The biological action of ALA has been ascribed to the characteristic antioxidant properties of the oxidized form (ALA) and its reduced counterpart the dihydrolipoic acid (DHLA) system. The ALA/DHLA combination represents an ideal antioxidant since it can quench radicals, is able to chelate metals, is amphiphilic, and has no major adverse effects. This unique system is able to scavenge reactive oxygen species, exerting a major effect on tissue levels of reduced forms of other antioxidants, including glutathione. For this reason, ALA is also known as the “antioxidant of antioxidants”. This review analyzes the antioxidant, anti-inflammatory, and neuroprotective effects of ALA and discusses its applications as an ameliorative tool for chronic diseases and those associated with oxidative stress. Results from in vitro and in vivo studies demonstrated that ALA modulates various oxidative stress pathways suggesting its application, alone or in combination with other functional substances, as a useful support in numerous conditions, in which the balance oxidant–antioxidant is disrupted, such as neurodegenerative disorders. Based on several successful clinical studies, it has been also established that oral ALA supplements are clinically useful in relieving the complications of diabetes and other disorders including cardiovascular diseases and nerve discomforts suggesting that ALA can be considered a useful approach to improving our health.
Comparative Study of an Antioxidant Compound and Ethoxyquin on Feed Oxidative Stability and on Performance, Antioxidant Capacity, and Intestinal Health in Starter Broiler Chickens
Authors: Xiao Yong , Gao Xuyang , Yuan Jianmin
Published: October 2024

Concerns over the safety of ethoxyquin (EQ) highlight the need for safer, more effective feed antioxidants. This study investigated a healthier antioxidant compound (AC) as a potential alternative to EQ in broilers. A total of 351 one-day-old Arbor Acres Plus male broilers were randomly assigned to three treatments for 21 days: control (CON), EQ group (200 g/ton EQ at 60% purity), and AC group (200 g/ton AC containing 18% butylated hydroxytoluene, 3% citric acid, and 1% tertiary butylhydroquinone). AC supplementation reduced the acid value, peroxide value, and malondialdehyde content in stored feed, decreased feed intake and the feed conversion ratio without affecting body weight gain, and enhanced antioxidant capacity (liver total antioxidant capacity and superoxide dismutase; intestinal catalase and glutathione peroxidase 7). It improved intestinal morphology and decreased barrier permeability (lower diamine oxidase and D-lactate), potentially by promoting ZO-1, Occludin, and Mucin2 expression. The AC also upregulated NF-κB p50 and its inhibitor (NF-κB p105), enhancing immune regulation. Additionally, the AC tended to increase beneficial gut microbiota, including Lactobacillus, and reduced Bacteroides, Corprococcus, and Anaeroplasma. Compared to EQ, the AC further enhanced feed oxidative stability, the feed conversion ratio, intestinal morphology and barrier functions, and inflammatory status, suggesting its potential as a superior alternative to EQ for broiler diets.
Green Extraction of Bioactive Compounds from Apple Pomace from the Cider Industry
Authors: Pando Bedriñana Rosa , Rodríguez Madrera Roberto , Loureiro Rodríguez María Dolores , López-Benítez Karelmar , Picinelli Lobo Anna
Published: October 2024

The cider-making industry in Asturias generates between 9000 and 12,000 tons of apple pomace per year. This by-product, the remains of the apple pressing, and made up of peel, flesh, seeds and stems, is a valuable material, containing substantial amounts of antioxidant compounds associated with healthy properties. Polyphenols such as dihydrochalcones and quercetin glycosides, and triterpenic acids, among which ursolic acid is a major compound, are the main antioxidant families described in apple pomace. The simultaneous recovery of those families has been accomplished by low frequency ultrasound-assisted extraction. Working extraction conditions were optimised by response surface methodology (RSM): time, 5.1 min; extractant composition, 68% ethanol in water; solid/liquid ratio, 1/75 and ultrasonic wave amplitude, 90%. This procedure was further applied to analyse those components in the whole apple pomace (WAP), apple peel (AP) and apple flesh (AF). On average, dry WAP contained almost 1300 µg/g of flavonols, 1200 µg/g of dihydrochalcones and 4200 µg/g of ursolic acid. These figures increased in the apple peel to, respectively 2500, 1400 and 8500 µg/g dry matter. Two linear multivariate regression models allowed the antioxidant activity of apple by-products to be predicted on the basis of their bioactive composition. The results derived from this study confirm the potential of industrial cider apple pomace as a source of high-value bioactive compounds, and the feasibility of the ultrasound-assisted extraction technique to recover those components in a simple and efficient way.
Evaluation of Glutathione (GSH) System in Porcine Saliva: Validation and Application of Colorimetric Method
Authors: Gok Gamze , Neselioglu Salim , Ko Heng-Lun , Erel Ozcan , López-Martínez María José , Manteca Xavier , Rubio Camila Peres
Published: October 2024

(1) Reduced glutathione (GSH) is considered the first line of antioxidant defense. During oxidative stress, it is oxidized to glutathione disulphide (GSSG). (2) A simple and quick spectrophotometric method based on sodium borohydride (NaBH4) as a reductant to measure the total and reduced GSH in porcine saliva was analytically validated and evaluated in two situations in this species: (a) in a physiological situation, involving sows during the late lactation and post-weaning periods, and (b) in a situation of sepsis in pigs experimentally induced by LPS administration. (3) The results of the analytical validation showed that the assay was precise and accurate in the porcine saliva samples. Higher total GSH and GSSG and lower reduced GSH were observed in the saliva of sows during the post-weaning period, as well as in pigs with experimentally induced sepsis. (4) In conclusion, the validated assay showed adequate analytical results and could be used to evaluate the GSH system of porcine saliva, as demonstrated during the clinical performance.
Reactive Oxygen and Nitrogen Species in Plants
Authors: Corpas Francisco J , Palma José M.
Published: October 2024

Reactive oxygen and nitrogen species (ROS and RNS) include two families of molecules that, in recent years, have been shown to be involved in a wide range of biological functions, such as seed and pollen germination, the development and regulation of root architecture, stomatal movement, senescence, flowering, and fruit formation and ripening [...]
Epicatechin Isolated from Litchi chinensis Sonn. (Litchi) Fruit Peel Ethyl Acetate Extract Modulated Glucose Uptake in Chang Cells and Suppressed ROS Production in RAW 264.7 Macrophages
Authors: Izu Gloria O. , Mapasa Nomonde P. , Nambooze Jennifer , Chukwuma Maria S. , Njoya Emmanuel Mfotie , Tabakam Gaetan T. , Bonnet Susanna L. , Makhafola Tshepiso J. , Mashele Samson S. , Chukwuma Chika I.
Published: October 2024

Bioactive flavonoid epicatechin has been reported in the peel of litchi fruit but isolated from its hydroalcoholic extracts. This study isolated epicatechin with cellular glucose uptake modulatory and ROS production inhibitory properties from the ethyl acetate (EtOAc) extract using a bioassay-guided approach. The fruit peel was defatted with hexane and sequentially extracted using dichloromethane (DCM), EtOAc, methanol (MeOH) and water. In vitro phytochemical models, namely antioxidant (Fe3+ reducing, radical scavenging and anti-linoleic acid peroxidative) and glycaemic control (α-glucosidase and α-amylase inhibitory and glucose uptake modulatory), were employed for the bioassay-guided isolation, while the isolated compound was characterised using NMR and mass spectrometry and assessed for dose-dependent inhibition of α-glucosidase and lipopolysaccharide (LPS)-induced cellular ROS production, as well as modulation of cellular glucose uptake. Relative to the other extracts, the EtOAc extract had appreciable phenol and flavonoid contents, which perhaps influenced its potent anti-lipid peroxidative (65.0%) and α-glucosidase inhibitory (52.4%) effects. The α-glucosidase inhibitory potency of the fractions (1–8) from the EtOAc extracts correlated with their flavonoid contents, with fraction 5 outperforming other fractions. The fraction comprised a pool of fractions obtained from the DCM:MeOH:water (7:3:0.281 v/v/v) solvent system. LC-MS revealed the predominant presence of epicatechin in fraction 5, which was later isolated from one of the sub-fractions (sub-fraction 4) of fraction 5. This sub-fraction had stronger anti-lipid peroxidative (65.5%), α-glucosidase inhibitory (65.8%) and glucose uptake modulatory (38.2%) effects than the other sub-fractions from fraction 5, which could have been influenced by the isolated epicatechin. Moreover, the isolated epicatechin inhibited α-glucosidase (IC50 = 35.3 µM), modulated cellular glucose uptake (EC50 = 78.5 µM) and inhibited LPS-induced ROS production in RAW 264.7 macrophages in a dose-dependent fashion [IC50 = 18.9 µM; statistically comparable (p > 0.05) to ascorbic acid, IC50 = 9.57 µM]. Epicatechin from litchi peel EtOAc extract could potentiate glucose uptake modulatory, α-glucosidase inhibitory and ROS suppressive capacities, which could be influential in the use of litchi fruit peel for managing diabetes and associated oxidative damage.