bluuebunny/crossref_metadata_2025 huggingface dataset:
Hard and Soft EM in Bayesian Network Learning from Incomplete Data
Authors: Ruggieri Andrea , Stranieri Francesco , Stella Fabio , Scutari Marco
Published: December 2020

Incomplete data are a common feature in many domains, from clinical trials to industrial applications. Bayesian networks (BNs) are often used in these domains because of their graphical and causal interpretations. BN parameter learning from incomplete data is usually implemented with the Expectation-Maximisation algorithm (EM), which computes the relevant sufficient statistics (“soft EM”) using belief propagation. Similarly, the Structural Expectation-Maximisation algorithm (Structural EM) learns the network structure of the BN from those sufficient statistics using algorithms designed for complete data. However, practical implementations of parameter and structure learning often impute missing data (“hard EM”) to compute sufficient statistics instead of using belief propagation, for both ease of implementation and computational speed. In this paper, we investigate the question: what is the impact of using imputation instead of belief propagation on the quality of the resulting BNs? From a simulation study using synthetic data and reference BNs, we find that it is possible to recommend one approach over the other in several scenarios based on the characteristics of the data. We then use this information to build a simple decision tree to guide practitioners in choosing the EM algorithm best suited to their problem.
Traffic Network Identification Using Trajectory Intersection Clustering
Authors: Gerdes Ingrid , Temme Annette
Published: December 2020

The current airspace route system consists mainly of pre-defined routes with a low number of intersections to facilitate air traffic controllers to oversee the traffic. Our aim is a method to create an artificial and reliable route network based on planned or as-flown trajectories. The application possibilities of the resulting network are manifold, reaching from the assessment of new air traffic management (ATM) strategies or historical data to a basis for simulation systems. Trajectories are defined as sequences of common points at intersections with other trajectories. All common points of a traffic sample are clustered, and, after further optimization, the cluster centers are used as nodes in the new main-flow network. To build almost-realistic flight trajectories based on this network, additional parameters such as speed and altitude are added to the nodes and the possibility to take detours into account to avoid congested areas is introduced. As optimization criteria, the trajectory length and the structural complexity of the main-flow system are used. Based on these criteria, we develop a new cost function for the optimization process. In addition, we show how different traffic situations are covered by the network. To illustrate the capabilities of our approach, traffic is exemplarily divided into separate classes and class-dependent parameters are assigned. Applied to two real traffic scenarios, the approach was able to emulate the underlying route systems with a difference in median trajectory length of 0.2%, resp. 0.5% compared to the original routes.
Developing Production Guidelines for Baby Leaf Hemp (Cannabis sativa L.) as an Edible Salad Green: Cultivar, Sowing Density and Seed Size
Authors: Mi Renyuan , Taylor Alan G. , Smart Lawrence B. , Mattson Neil S.
Published: December 2020

Scientific literature is lacking on cultural practices of baby leaf hemp production even though hemp (Cannabis sativa L.) is a widely grown crop for fiber and grain. The objective of this study was to develop a standard protocol to optimize yield and quality of baby leaf hemp production: cultivar screening, sowing density and seed size. Fresh weight (FW) and germination percentage was significantly affected by cultivars. Cultivars ‘Picolo’ and ‘X-59’ had a greater FW mainly due to greater germination percentage. In the sowing density experiment, ‘Ferimon’ and ‘Katani’ were evaluated at five seed densities, 0.65, 1.2, 1.75, 2.3 and 2.85 seeds·cm−2 (42 to 182 seeds per cell). The FW and FW per plant (FWPP) had a positive quadratic response and negative quadratic response, respectively. Regarding seed size, cultivars ‘Anka,’ ‘Ferimon’ and ‘Picolo’ had the largest percentage of seeds, 26% to 30%, within the medium width size between 3.18 and 3.37 mm. Using the largest sized seeds (3.77 mm) increased FW by 34%, 26% and 23% as compared to non-sorted ‘Anka’, ‘Ferimon’ and ‘Picolo’ seeds, respectively. Overall, a greater understanding of cultivar selection, sowing density and seed-size distribution can promote greater yield and quality of baby leaf hemp as an edible salad green.
A Biostimulant Based on Protein Hydrolysates Promotes the Growth of Young Olive Trees
Authors: Almadi Leen , Paoletti Andrea , Cinosi Nicola , Daher Elissa , Rosati Adolfo , Di Vaio Claudio , Famiani Franco
Published: December 2020

Experiments were carried out in 2018 and 2019 to evaluate the effects of a biostimulant of animal origin, with a hormone-like action, on the growth and physiology of young olive trees. The biostimulant, consisting of a complex of natural amino acids (glycine, proline, and hydroxyproline, etc.), was applied by fertigation to both potted (one-year-old) and field-grown (2 years after transplanting) young olive trees. The control consisted of trees treated with an amount of urea nitrogen equal to the total nitrogen supplied with the biostimulant. Potted trees treated with the biostimulant showed greater growth than the control soon after treatment and up to the end of the vegetative season. Generally, this was associated with higher leaf photosynthetic rates and stomatal conductance. At the end of the experiment, all tree parts (roots, stem, shoots, and leaves) of the treated trees had greater biomass than the control. The trunks of field-grown trees treated with the biostimulant had greater growth with respect to the control. Overall, the results indicate the possibility of using the biostimulant based on complexes of natural amino acids to promote the growth of young olive trees.
Assessing the Standard of Health and Welfare in Individual Categories of Cattle from the Viewpoint of Intravital Pathological Changes
Authors: Kaluza Michal , Vecerek Vladimir , Voslarova Eva , Semerad Zbynek , Passantino Annamaria
Published: December 2020

The standard of the health and welfare of individual categories of cattle was assessed from the viewpoint of intravital pathological changes. The results of veterinary inspections of 2,514,666 head of cattle (1,136,754 cows, 257,912 heifers, 1,015,541 bulls and 104,459 calves) slaughtered in the Czech Republic in the period from 2010 to 2019 were analyzed. The results show significant differences (p < 0.01) between the categories of cattle in the total numbers of intravital findings. Intravital findings were most frequently recorded in cows (index 2.271) and least frequently recorded in bulls (index 0.479). In all categories of cattle, chronic findings predominated, where the largest number was recorded in cows (index 1.740) and calves (index 0.910). Parasitic and traumatic findings were only detected in low numbers in all categories of cattle. Assessment of the trend in the total number of intravital pathological findings in individual categories of cattle in the monitored period showed an evident fall in heifers (rSp = −0.915, p < 0.01), cows (rSp = −0.806, p < 0.01) and bulls (rSp = −0.636, p < 0.05). In calves, no decreasing trend was found (rSp = −0.382, p > 0.05). The results obtained in this study do not merely reflect the standard of health of the animals slaughtered at abattoirs in the Czech Republic, but also provide a picture of the standard of the living conditions provided by farmers rearing individual categories of cattle.
Genomic Characteristics of Colistin-Resistant Salmonella enterica subsp. enterica Serovar Infantis from Poultry Farms in the Republic of Serbia
Authors: Jovčić Branko , Novović Katarina , Filipić Brankica , Velhner Maja , Todorović Dalibor , Matović Kazimir , Rašić Zoran , Nikolić Sonja , Kiškarolj Ferenc , Kojić Milan
Published: December 2020

The antimicrobial susceptibility testing was conducted on 174 single isolates from poultry farms in Serbia and it was determined that seven Salmonella spp. were multidrug resistant. Sixteen serotypes were detected, but only serotype Infantis confirmed reduced susceptibility to colistin. Seven colistin resistant Salmonella Infantis were studied in detail using the WGS approach. Three sequence types were identified corresponding to different epizootiology region. The isolate from the Province of Vojvodina 3842 and isolates from Jagodina (92 and 821) are represented by the sequence type ST413 and ST11, respectively. Four isolates from Kraljevo are ST32, a common S. Infantis sequence type in humans, poultry and food. The fosfomycin resistance gene fosA7 in isolate 3842 and the vgaA gene in isolate 8418/2948 encoding resistance to pleuromutilins were reported for the first time in serovar Infantis. The changes in relative expression of the phoP/Q, mgrB and pmrA/B genes were detected. Single nucleotide polymorphisms of the pmrB gene, including transitions Val164Gly or Val164Met, and Arg92Pro are described. Analyses of quinolone resistance determining region revealed substitutions Ser83Tyr in GyrA protein and Thr57Ser and Ser80Arg in ParC protein. Based on WGS data, there are two major clusters among analyzed Salmonella Infantis isolates from central Serbia.
Finding the Dose for Ceftolozane-Tazobactam in Critically Ill Children with and without Acute Kidney Injury
Authors: Butragueño-Laiseca Laura , Troconiz Iñaki F. , Grau Santiago , Campillo Nuria , García Xandra , Padilla Belén , Fernández Sarah N. , Santiago María José
Published: December 2020

Background: Ceftolozane-tazobactam is a new antibiotic against multidrug-resistant pathogens such as Pseudomonas aeruginosas. Ceftolozane-tazobactam dosage is still uncertain in children, especially in those with renal impairment or undergoing continuous renal replacement therapy (CRRT). Methods: Evaluation of different ceftolozane-tazobactam dosing regimens in three critically ill children. Ceftolozane pharmacokinetics (PK) were characterized by obtaining the patient’s specific parameters by Bayesian estimation based on a population PK model. The clearance (CL) in patient C undergoing CRRT was estimated using the prefilter, postfilter, and ultrafiltrate concentrations simultaneously. Variables such as blood, dialysate, replacement, and ultrafiltrate flow rates, and hematocrit were integrated in the model. All PK analyses were performed using NONMEM v.7.4. Results: Patient A (8 months of age, 8.7 kg) with normal renal function received 40 mg/kg every 6 h: renal clearance (CLR) was 0.88 L/h; volume of distribution (Vd) Vd1 = 3.45 L, Vd2 = 0.942 L; terminal halflife (t1/2,β) = 3.51 h, dosing interval area under the drug concentration vs. time curve at steady-state (AUCτ,SS) 397.73 mg × h × L−1. Patient B (19 months of age, 11 kg) with eGFR of 22 mL/min/1.73 m2 received 36 mg/kg every 8 h: CLR = 0.27 L/h; Vd1 = 1.13 L; Vd2 = 1.36; t1/2,β = 6.62 h; AUCSS 1481.48 mg × h × L−1. Patient C (9 months of age, 5.8 kg), with severe renal impairment undergoing CRRT received 30 mg/kg every 8 h: renal replacement therapy clearance (CLRRT) 0.39 L/h; Vd1 = 0.74 L; Vd2= 1.17; t 1/2,β = 3.51 h; AUCτ,SS 448.72 mg × h × L−1. No adverse effects attributable to antibiotic treatment were observed. Conclusions: Our results suggest that a dose of 35 mg/kg every 8 h can be appropriate in critically ill septic children with multi-drug resistance Pseudomonas aeruginosa infections. A lower dose of 10 mg/kg every 8 h could be considered for children with severe AKI. For patients with CRRT and a high effluent rate, a dose of 30 mg/kg every 8 h can be considered.
Efficacy of Daptomycin-Containing Regimen for Treatment of Staphylococcal or Enterococcal Vertebral Osteomyelitis: A Prospective Clinical Experience
Authors: Russo Alessandro , Ceccarelli Giancarlo , Bellelli Valeria , Bianchi Luigi , Marincola Cattaneo Federica , Gregori Fabrizio , Palmarini Valeria , Marotta Nicola , Landi Alessandro , Cuzzolino Alessandro , Stefanini Matteo , Aureli Alessandro , Mastroianni Claudio Maria , Venditti Mario , d’Ettorre Gabriella , Sabetta Francesco
Published: December 2020

Vertebral osteomyelitis (VO) is a compelling clinical entity for clinicians, because of its insidious and indolent course that makes diagnosis difficult. A concern is reported about the choice of antibiotic regimens, duration of therapy, and criteria to switch to oral therapy. We conducted a prospective observational study. All consecutive hospitalized patients with a confirmed diagnosis of VO caused by staphylococcal or enterococcal strains were analyzed. The primary endpoint was the analysis of clinical cure at the end of therapy. A propensity score for receiving therapy with daptomycin was added to the model. During the study period, 60 episodes of confirmed VO were observed. The main etiology of infection was methicillin-resistant Staphylococcus aureus (29%). Overall, clinical failure at end of therapy was reported in 11 (18.3%) patients. Logistic regression analysis, after propensity score, showed that >2 vertebrae involved (OR 2.4, CI95% 1.12–5.24, p = 0.002) and inadequate drainage of infection (OR 4.8, CI95% 2.45–8.51, p < 0.001) were independently associated with failure of therapy, while the use of a daptomycin-containing-regimen (OR 0.15, CI 95% 0.04–0.46, p < 0.001) with clinical cure. VO caused by staphylococcal or enterococcal strains is associated with an important rate of clinical failure. Daptomycin-containing regimen was strongly associated with clinical cure. Considering that over 70% of VO etiology is caused by Gram-positive strains but the etiology of infection is obtained in about 75% of cases, these data may help physicians to choose the appropriate antibiotic regimen.
Myeloperoxidase Modulates Hydrogen Peroxide Mediated Cellular Damage in Murine Macrophages
Authors: Guo Chaorui , Sileikaite Inga , Davies Michael J. , Hawkins Clare L.
Published: December 2020

Myeloperoxidase (MPO) is involved in the development of many chronic inflammatory diseases, in addition to its key role in innate immune defenses. This is attributed to the excessive production of hypochlorous acid (HOCl) by MPO at inflammatory sites, which causes tissue damage. This has sparked wide interest in the development of therapeutic approaches to prevent HOCl-induced cellular damage including supplementation with thiocyanate (SCN−) as an alternative substrate for MPO. In this study, we used an enzymatic system composed of glucose oxidase (GO), glucose, and MPO in the absence and presence of SCN−, to investigate the effects of generating a continuous flux of oxidants on macrophage cell function. Our studies show the generation of hydrogen peroxide (H2O2) by glucose and GO results in a dose- and time-dependent decrease in metabolic activity and cell viability, and the activation of stress-related signaling pathways. Interestingly, these damaging effects were attenuated by the addition of MPO to form HOCl. Supplementation with SCN−, which favors the formation of hypothiocyanous acid, could reverse this effect. Addition of MPO also resulted in upregulation of the antioxidant gene, NAD(P)H:quinone acceptor oxidoreductase 1. This study provides new insights into the role of MPO in the modulation of macrophage function, which may be relevant to inflammatory pathologies.
First Evidence of a Protective Effect of Plant Bioactive Compounds against H2O2-Induced Aconitase Damage in Durum Wheat Mitochondria
Authors: Laus Maura N. , Soccio Mario
Published: December 2020

In order to contribute to the understanding of the antioxidant behavior of plant bioactive compounds with respect to specific subcellular targets, in this study, their capability to protect aconitase activity from oxidative-mediated dysfunction was evaluated for the first time in plant mitochondria. Interest was focused on the Krebs cycle enzyme catalyzing the citrate/isocitrate interconversion via cis-aconitate, as it possesses a [4Fe-4S]2+ cluster at the active site, making it an early and highly sensitive target of reactive oxygen species (ROS)-induced oxidative damage. In particular, the effect on the aconitase reaction of five natural phenols, including ferulic acid, apigenin, quercetin, resveratrol, and curcumin, as well as of the isothiocyanate sulforaphane, was investigated in highly purified mitochondria obtained from durum wheat (DWM). Interestingly, a short-term (10 min) DWM pre-treatment with all investigated compounds, applied at 150 µM (75 µM in the case of resveratrol), completely prevented aconitase damage induced by a 15 min exposure of mitochondria to 500 µM H2O2. Curcumin and quercetin were also found to completely recover DWM-aconitase activity when phytochemical treatment was performed after H2O2 damage. In addition, all tested phytochemicals (except ferulic) induced a significant increase of aconitase activity in undamaged mitochondria. On the contrary, a relevant protective and recovery effect of only quercetin treatment was observed in terms of the aconitase activity of a commercial purified mammalian isoform, which was used for comparison. Overall, the results obtained in this study may suggest a possible role of phytochemicals in preserving plant mitochondrial aconitase activity, as well as energy metabolism, against oxidative damage that may occur under environmental stress conditions. Further investigations are needed to elucidate the physiological role and the mechanism responsible for this short-term protective effect.
Thioredoxin and Glutaredoxin Systems as Potential Targets for the Development of New Treatments in Friedreich’s Ataxia
Authors: Seco-Cervera Marta , González-Cabo Pilar , Pallardó Federico , Romá-Mateo Carlos , García-Giménez José
Published: December 2020

The thioredoxin family consists of a small group of redox proteins present in all organisms and composed of thioredoxins (TRXs), glutaredoxins (GLRXs) and peroxiredoxins (PRDXs) which are found in the extracellular fluid, the cytoplasm, the mitochondria and in the nucleus with functions that include antioxidation, signaling and transcriptional control, among others. The importance of thioredoxin family proteins in neurodegenerative diseases is gaining relevance because some of these proteins have demonstrated an important role in the central nervous system by mediating neuroprotection against oxidative stress, contributing to mitochondrial function and regulating gene expression. Specifically, in the context of Friedreich’s ataxia (FRDA), thioredoxin family proteins may have a special role in the regulation of Nrf2 expression and function, in Fe-S cluster metabolism, controlling the expression of genes located at the iron-response element (IRE) and probably regulating ferroptosis. Therefore, comprehension of the mechanisms that closely link thioredoxin family proteins with cellular processes affected in FRDA will serve as a cornerstone to design improved therapeutic strategies.
Nanoporous Activated Carbon Derived via Pyrolysis Process of Spent Coffee: Structural Characterization. Investigation of Its Use for Hexavalent Chromium Removal
Authors: Asimakopoulos Georgios , Baikousi Maria , Kostas Vasilis , Papantoniou Marios , Bourlinos Athanasios B. , Zbořil Radek , Karakassides Michael A. , Salmas Constantinos E.
Published: December 2020

Hexavalent chromium (Cr(VI)) is a heavy metal that is highly soluble and exhibits toxic effects on biological systems. Nevertheless, it is used in many industrial applications. The adsorption process of Cr(VI), using activated carbon (AC), is under investigation globally. On the other hand, around six million tons of spent coffee is sent to landfill annually. In the spirit of cyclic economy, this research investigated the production of AC from spent coffee for the removal of Cr(VI) from wastewater. The AC was produced via pyrolysis process under a nitrogen atmosphere. Chemical activation using potassium hydroxide (KOH) occurred simultaneously with the pyrolysis process. The produced AC was tested as an absorber of Cr(VI). The best fitted kinetic model was the diffusion–chemisorption model. A 24-h adsorption experiment was carried out using a solution with a pH of 3 and an initial Cr(VI) concentration of 54.14 ppm. This resulted in an experimental maximum capacity of 109 mg/g, while the theoretical prediction was 137 mg/g. It also resulted in an initial adsorption rate (ri) of 110 (mg/(g h)). The Brunauer–Emmett–Teller surface area (SgBET) was 1372 m2/g, the Langmuir surface area (SgLang.) was 1875 m2/g, and the corrugated pore structure model surface area (SgCPSM) was 1869 m2/g. The micropore volume was 84.6%, exhibiting micropores at Dmicro1 = 1.28 and Dmicro2 = 1.6 nm. The tortuosity factor (τ) was 4.65.
Graphic Applications of Unmanned Aerial Vehicles (UAVs) in the Study of Industrial Heritage Assets
Authors: Martín-Béjar Sergio , Claver Juan , Sebastián Miguel A. , Sevilla Lorenzo
Published: December 2020

As a typology of cultural heritage, a wide range of singularities must to be taken into account when analysing industrial heritage assets. Graphical resources have been usually employed in heritage assets research. Nowadays, Unmanned Aerial Vehicles (UAVs) offer the possibility to obtain images of complex and difficult access areas that cannot be observed on the ground. Furthermore, aerial images allow to analyse heritage assets as a whole. Therefore, in this work, different photographs and videos has been carried out in the San Joaquin sugar cane factory and in the Aguila aqueduct, located in Nerja (Spain). These images have been used to analyse both assets as a set. Different indirect measurements on the factory chimney and irrigation water pond were taken. A Strengths, Weaknesses, Opportunities, and Threats (SWOT) about the use of UAV graphic applications in heritage assets was developed. Finally, the possibilities of conventional photograph technologies and UAV application were compared. Using UAVs allows more image acquisition possibilities than conventional systems. However, both technologies can be complemented and applied together when analysing heritage assets.
Adsorption of NO2 and H2S on ZnGa2O4(111) Thin Films: A First-Principles Density Functional Theory Study
Authors: Tung Jen-Chuan , Chiang Yi-Hung , Wang Ding-Yuan , Liu Po-Liang
Published: December 2020

We performed first-principles total-energy density functional calculations to study the reactions of NO2 and H2S molecules on Ga–Zn–O-terminated ZnGa2O4(111) surfaces. The adsorption reaction and work functions of eight NO2 and H2S adsorption models were examined. The bonding of the nitrogen atom from a single NO2 molecule to the Ga atom of the Ga–Zn–O-terminated ZnGa2O4(111) surfaces exhibited a maximum work function change of +0.97 eV. The bond joining the sulfur atom from a single H2S molecule and the Ga atom of Ga–Zn–O-terminated ZnGa2O4(111) surfaces exhibited a maximum work function change of −1.66 eV. Both results concur with previously reported experimental observations for ZnGa2O4-based gas sensors.
Modeling and Speed Tuning of a PMSM with Presence of Fissure Using Dragonfly Algorithm
Authors: Aguilar-Mejía Omar , Manilla-García Abraham , Rivas-Cambero Ivan , Minor-Popocatl Hertwin
Published: December 2020

This paper presents a robust trajectory tracking control for a Permanent Magnet Synchronous Motor (PMSM) with consideration a fault, parametric uncertainties and external disturbances by effectively integrating robust optimal linear quadratic control. One kind of fault is considered in the machine, particularly the presence of fissure rotor. The dynamic model of the PMSM with the presence of fissure presents highly non-linear behaviors, which means that tuning is quite complicated, which the tuning was chosen through swarm intelligence optimization (Dragonfly Algorithm). A sensitivity analysis is carried out, in order to limit the search range to minimize the evaluation time. This methodology was used to diminish these defects during motor operation. Simulation results show that the optimal linear quadratic control method has a robust fault-tolerant performance.
Influence of Rainfall Intensity on the Stability of Unsaturated Soil Slope: Case Study of R523 Road in Thulamela Municipality, Limpopo Province, South Africa
Authors: Sengani Fhatuwani , Mulenga François
Published: December 2020

The purpose of this paper was to analyze the impact of extreme rainfall on the recurrence of slope instability using the Thulamela Municipality roads (R523) as a case study. To this end, the historical rainfall data of the area of study were analyzed between 1988 and 2018. The results show that a significant increase in rainfall is usually experienced in the summer months of December and January. Following this, the factor of safety (FoS) of slopes of silt clay, clay, and clay loam soils were estimated using the SLIDE simulator (Numerical software “Finite Element Method (FEM)”) under sunny to rainy conditions of the area. A complementary model, FLACSlope (Numerical software “Finite Difference Method (FDM)”), was utilized to simulate FoS and pore water pressure in sunny and rainy conditions of the area. Simulation results show that extreme rainfall has the ability to reduce the shear strength and resistance of the soil slope material. This may explain the recurrent landslides noted in the area. Finally, the water pore pressure has been simulated to increase with the increased water table, which generally pushes the soil particles apart and reduces the stress between the particles resulting in soil slope failure. Extreme rainfall alters the phase of the material solid in a manner that may require further research for a better understanding.
A Haptic Feedback Actuator Suitable for the Soft Wearable Device
Authors: Ma Jiaqi , Cheng Xiang , Wang Pengfei , Jiao Zhiwei , Yu Yuan , Yu Meng , Luo Bin , Yang Weimin
Published: December 2020

Gaining direct tactile sensation is becoming increasingly important for humans in human–computer interaction fields such as space robot teleoperation and augmented reality (AR). In this study, a novel electro-hydraulic soft actuator was designed and manufactured. The proposed actuator is composed of polydimethylsiloxane (PDMS) films, flexible electrodes, and an insulating liquid dielectric. The influence of two different voltage loading methods on the output characteristics of the actuator was studied. The special voltage loading method (AC voltage) enables the actuator to respond rapidly (within 0.15 s), output a stable displacement in 3 s, and remain unchanged in the subsequent time. By adjusting the voltages and frequencies, a maximum output displacement of 1.1 mm and an output force of 1 N/cm2 can be rapidly achieved at a voltage of 12 kV (20 Hz). Finally, a haptic feedback system was built to control the robotic hand to perform gripping tasks in real time, and a more realistic tactile sensation could be realized, similar to that obtained when a human directly grabs objects. Therefore, the actuator has excellent portability, robustness, rapid response, and good compatibility with the human body for human–computer interaction.
Highly Directive Biconic Antennas Embedded in a Dielectric
Authors: Chiolerio Alessandro , Diazzi Lorenzo , Funaro Daniele
Published: December 2020

Designing antennas suitable for generating highly directive electromagnetic signals has become a fundamental task. This is particularly relevant for the development of efficient and sustainable point-to-point communication channels, and for energy transfer. Indeed, these are nowadays expanding areas of research. In order to deal with said particular wave phenomena, an extension of the electrodynamics equations is taken into account, where exact solitonic type solutions are admitted. These waves may have compact support and travel along a straight line, without dissipation, at the speed of light. The result suggests the design of biconic type antennas having specific properties that are numerically examined in this paper. The cones, supplied with an oscillating source, are embedded in a dielectric material of suitable shape, with the purpose of driving the signal in the proper direction. The computations based on the extended model are aimed toward simulating the possibility of generating peculiar wave behaviors, in view of practical implementations in the framework of point-to-point communications or wireless power transmission.
Supramaximal Eccentric Training for Alpine Ski Racing—Strength Training with the Lifter
Authors: Patterson Carson , Raschner Christian
Published: December 2020

Eccentric muscular work plays a large role in alpine ski racing. Training with supramaximal eccentric loads (SME) is highly effective to improve eccentric strength but potentially dangerous. Most SME training devices do not allow the athlete to move a barbell freely as they would when performing conventional barbell training. The Intelligent Motion Lifter (IML) allows for safe SME training with a free barbell and no spotters. The IML can be used for free barbell training: a spotter for normal training, eccentric only, concentric only, and squat jumps. It is also a training and testing device for isokinetic and isometric exercise. This commentary addresses the necessity of eccentric training for elite alpine ski racers, the development of the IML and its use in training.
Long-Range Acoustic Communication Using Differential Chirp Spread Spectrum
Authors: Lee Joohyoung , An Jeongha , Ra Hyung-in , Kim Kiman
Published: December 2020

Here, we propose a new modulation method using chirp spread spectrum (CSS) modulation to indicate the result of long-range acoustic communication (LRAC). CSS modulation had outstanding matched filter characteristics even though the channel was complex. The performance of the matched filter depends on the time–bandwidth product. We studied the method of using the same modulation method while increasing the amount of the time–bandwidth product. When differential encoding is applied, the de-modulation is made using the difference between the current symbol and the previous symbol. If the matched filter is applied using both the current and the previous symbol, such as the use of two symbols, the amount of the time–bandwidth product can be doubled, and this method can make the output of the matched filter larger. The proposed method was verified in lake and sea experiments, in which the experimental environment was analyzed and compared with the result using the channel impulse response (CIR) of the lake and sea. The lake experiment was conducted over a distance of about 100–300 m between the transmitter and receiver and at a depth of ~40 m. As a result of the communication, the conventional method’s bit error rate (BER) was 1.22×10−1, but the proposed method’s BER was 1.98×10−2. The sea experiment was conducted over a distance of ~90 km and at a depth of ~1 km, and the conventional method BER in this experiment was 1.83×10−4, while the proposed method’s BER was 0.
Postural Evaluation in Sports and Sedentary Subjects by Rasterstereographic Back Shape Analysis
Authors: Bernetti Andrea , Agostini Francesco , Cacchio Angelo , Santilli Valter , Ruiu Pierangela , Paolucci Teresa , Paoloni Marco , Mangone Massimiliano
Published: December 2020

Posture is defined as the position of the body in space, the aim of which is to maintain balance, both in static and dynamic conditions. Our purpose was to study various postural variables involved in postural adaptations of athletes practicing symmetric and asymmetric sports at professional level. Methods: Patients include sedentary subjects, competitive athletes practicing symmetrical and asymmetrical sports. Postural evaluation of the three different groups was performed using the rasterstereographic-system Formetric-4D. Results: 157 subjects were recruited. From the comparison between subjects playing symmetrical and asymmetrical sports, arises a statistically significant difference on cervical (p = 0.041) and lumbar (p = 0.047) flèche of Stagnara, with higher values for symmetrical athletes’ group. Hemipelvis torsion (p = 0.031) and lumbar flèche (p ≤ 0.001) of Stagnara are higher in symmetrical athletes’ group (sedentary). Hemipelvis torsion, cervical and lumbar flèche resulted to be higher among athletes (sedentary) (p = 0.016, p = 0.003, p = 0.027). Conclusions: In addition to the competitive sports’ medical examination, a screening with rasterstereographic-system Formetric-4D is suggested to all sedentary subjects, without serious skeletal pathologies which want to start athletic activity. Rasterstereographic-system Formetric-4D is also suggested to all athletes practicing sports, with the aim to identify eventual unknown postures, consequent to reiterated repetition of specific movements.
Model Predictive Control Method Based on Deterministic Reference Voltage for Single-Phase Three-Level NPC Converters
Authors: Jun Eun-Su , Nguyen Minh Hoang , Kwak Sangshin
Published: December 2020

When single-phase three-level neutral-point-clamped (NPC) converters operate, there are two main control objectives that need to be met for correct operation. First, the ac source current must be controlled to be sinusoidal. Second, the dc capacitor voltages must be balanced. In original model predictive control (MPC) methods for NPC converters, an optimization process involving an empirical weighting factor design is required to meet both of these objectives simultaneously. This study proposes an MPC approach developed for single-phase three-level NPC converters to meet these objectives using a single reference voltage consisting of a difference-mode term and a common-mode term in each phase. The difference-mode term and the common-mode term are responsible for sinusoidal ac source current synthesis and dc capacitor voltage balancing, respectively. Then, a single cost function compares the adjusted reference voltage with possible voltage candidates to select an optimal switching state, resulting in the smallest cost function value. Different from the conventional MPC method, the proposed approach avoids the selection of weighting factors and the attendance of various control objectives. Thanks to the deterministic approach, the proposed MPC method is straightforward to implement and maintain fast transient performance while guaranteeing the control objectives. Finally, the effectiveness and feasibility of the proposed approach for single-phase three-level NPC are verified through comprehensive experimental results.
Safe Three-Dimensional Assembly Line Design for Robots Based on Combined Multiobjective Approach
Authors: Wang Shuai , Guo Ruifeng , Wang Hongliang , Vogel-Heuser Birgit
Published: December 2020

In advanced industrial automation, industrial robots have been widely utilized on assembly lines in order to reduce labor dependence. However, many related layout design approaches proposed are prone to generating unsafe layouts: there generally lacks a consideration regarding robots’ heights and assembly range, which will lead to costly collisions in the operation stage. In order to address the problem, we propose a three-dimensional (3D) optimization approach to a safe layout design for an assembly line with robots. We define modeling rules for robots to judge assembly ranges. A quantitative safety indicator is employed as a trigger for 3D collision detection in order to determine the positional relationship and status of the safe assembly collaboration. The optimization goals are established for minimizing the logistical cost and layout area in the model. A combined algorithm of differential evolution and nondominated sequencing genetic II is applied, which effectively enhances the poor diversity and convergence of the mainstream optimization method when solving this model. The benchmark tests and validation proved that our approach yields excellent convergence and distribution performance. The case study verifies that the safe layout model is valid and our approach can generate a safe layout in order to optimize economics and safety.
A Systematic Comparative Study of the Toxicity of Semiconductor and Graphitic Carbon-Based Quantum Dots Using In Vitro Cell Models
Authors: Navarro-Ruiz Maria Carmen , Cayuela Angelina , Soriano María Laura , Guzmán-Ruiz Rocio , Malagón Maria M. , Valcárcel Miguel
Published: December 2020

A comparative, fully parallel study of nanoparticles (NPs) toxicity by in vitro cell viability is shown looking for reliable comparability of nanotoxicological results, a well-recognized bottleneck in the context. This procedure is suitable to compare toxicity of similar NPs, as well as the influence on toxicity of the size, surface, and other characteristics. As a case of study, semiconductor (SQDs) and graphitic-carbon quantum dots (CQDs) with identical surface groups and size were evaluated. All experiments were conducted at same conditions, involving two types of cells (mouse fibroblasts (3T3-L1) and carcinoma human hepatocellular cells (HepG2)) and different extracellular components (in the absence or presence of fetal bovine serum (FBS)). Cell viability demonstrated the excellent biocompatibility of CQDs compared to SQDs, which caused higher percentage of cell death at lower concentrations, as predicted but never clearly demonstrated. However, our comparative studies established that the toxicity of SQDs and CQDs are cellular type-dependent, and the absence or presence of serum proteins reduces the minimal concentration necessary of NPs to produce toxicity.
Estimation on Embedment Length of Anchor Bolt inside Concrete Using Equation for Arrival Time and Shortest Time Path of Ultrasonic Pulse
Authors: Song Chiwon , Kim Young Jin , Cho Chang Beck , Chin Won Jong , Park Kwang-Yeun
Published: December 2020

The bearings or the seismic isolation bearings that play a critical role in bridge structures are fixed to the substructure by anchor bolts. However, the embedment depth of the constructed anchor bolts does often not reach the designed one and may lead to safety issues. The present study proposes an ultrasonic non-destructive testing (NDT) method to verify the embedment depth of the anchor bolts installed on bridges in-service. The P-wave of 50–100 kHz that is usually used in the NDT of concrete was transmitted from the head of the anchor bolt and its arrival time on the concrete cover was measured. The shortest arrival time of the ultrasonic pulse and the corresponding path were then analyzed to formulate their relationship and obtain the distance traveled by the ultrasonic pulse along the anchor by inverse analysis using the equation error estimation. The instability occurring in the inverse analysis is settled by regularization. Finally, the embedment depth of the anchor bolt can be estimated by the analysis of the graph plotting the position of the ultrasonic transmitter and the distance traveled by the pulse along the anchor. The proposed method is validated numerically and experimentally. The method is expected to contribute to the NDT of civil structures by making it possible to estimate the embedment depth of anchor bolts by the means of ultrasonic transducers using P-waves of 50–100 kHz.
Quantum Photonic Simulation of Spin-Magnetic Field Coupling and Atom-Optical Field Interaction
Authors: Liñares Jesús , Prieto-Blanco Xesús , Carral Gabriel M. , Nistal María C.
Published: December 2020

In this work, we present the physical simulation of the dynamical and topological properties of atom-field quantum interacting systems by means of integrated quantum photonic devices. In particular, we simulate mechanical systems used, for example, for quantum processing and requiring a very complex technology such as a spin-1/2 particle interacting with an external classical time-dependent magnetic field and a two-level atom under the action of an external classical time-dependent electric (optical) field (light-matter interaction). The photonic device consists of integrated optical waveguides supporting two collinear or codirectional modes, which are coupled by integrated optical gratings. We show that the single-photon quantum description of the dynamics of this photonic device is a quantum physical simulation of both aforementioned interacting systems. The two-mode photonic device with a single-photon quantum state represents the quantum system, and the optical grating corresponds to an external field. Likewise, we also present the generation of Aharonov–Anandan geometric phases within this photonic device, which also appear in the simulated systems. On the other hand, this photonic simulator can be regarded as a basic brick for constructing more complex photonic simulators. We present a few examples where optical gratings interacting with several collinear and/or codirectional modes are used in order to illustrate the new possibilities for quantum simulation.
Multimode Collective Atomic Recoil Lasing in Free Space
Authors: Gisbert Angel T. , Piovella Nicola
Published: December 2020

Cold atomic clouds in collective atomic recoil lasing are usually confined by an optical cavity, which forces the light-scattering to befall in the mode fixed by the resonator. Here we consider the system to be in free space, which leads into a vacuum multimode collective scattering. We show that the presence of an optical cavity is not always necessary to achieve coherent collective emission by the atomic ensemble and that a preferred scattering path arises along the major axis of the atomic cloud. We derive a full vectorial model for multimode collective atomic recoil lasing in free space. Such a model consists of multi-particle equations capable of describing the motion of each atom in a 2D/3D cloud. These equations are numerically solved by means of molecular dynamic algorithms, usually employed in other scientific fields. The numerical results show that both atomic density and collective scattering patterns are applicable to the cloud’s orientation and shape and to the polarization of the incident light.
Future Perspectives in Small-Diameter Vascular Graft Engineering
Authors: Mallis Panagiotis , Kostakis Alkiviadis , Stavropoulos-Giokas Catherine , Michalopoulos Efstathios
Published: December 2020

The increased demands of small-diameter vascular grafts (SDVGs) globally has forced the scientific society to explore alternative strategies utilizing the tissue engineering approaches. Cardiovascular disease (CVD) comprises one of the most lethal groups of non-communicable disorders worldwide. It has been estimated that in Europe, the healthcare cost for the administration of CVD is more than 169 billion €. Common manifestations involve the narrowing or occlusion of blood vessels. The replacement of damaged vessels with autologous grafts represents one of the applied therapeutic approaches in CVD. However, significant drawbacks are accompanying the above procedure; therefore, the exploration of alternative vessel sources must be performed. Engineered SDVGs can be produced through the utilization of non-degradable/degradable and naturally derived materials. Decellularized vessels represent also an alternative valuable source for the development of SDVGs. In this review, a great number of SDVG engineering approaches will be highlighted. Importantly, the state-of-the-art methodologies, which are currently employed, will be comprehensively presented. A discussion summarizing the key marks and the future perspectives of SDVG engineering will be included in this review. Taking into consideration the increased number of patients with CVD, SDVG engineering may assist significantly in cardiovascular reconstructive surgery and, therefore, the overall improvement of patients’ life.
Apoptosis of Eosinophil Granulocytes
Authors: Zustakova Martina , Kratochvilova Lucie , Slama Petr
Published: December 2020

In the past 10 years, the number of people in the Czech Republic with allergies has doubled to over three million. Allergic pollen catarrh, constitutional dermatitis and asthma are the allergic disorders most often diagnosed. Genuine food allergies today affect 6–8% of nursing infants, 3–5% of small children, and 2–4% of adults. These disorders are connected with eosinophil granulocytes and their apoptosis. Eosinophil granulocytes are postmitotic leukocytes containing a number of histotoxic substances that contribute to the initiation and continuation of allergic inflammatory reactions. Eosinophilia results from the disruption of the standard half-life of eosinophils by the expression of mechanisms that block the apoptosis of eosinophils, leading to the development of chronic inflammation. Glucocorticoids are used as a strong acting anti-inflammatory medicine in the treatment of hypereosinophilia. The removal of eosinophils by the mechanism of apoptosis is the effect of this process. This work sums up the contemporary knowledge concerning the apoptosis of eosinophils, its role in the aforementioned disorders, and the indications for the use of glucocorticoids in their related therapies.
Discoveries for Long Non-Coding RNA Dynamics in Traumatic Brain Injury
Authors: Lim Key-Hwan , Yang Sumin , Kim Sung-Hyun , Chun Sungkun , Joo Jae-Yeol
Published: December 2020

In recent years, our understanding of long non-coding RNAs (lncRNAs) has been challenged with advances in genome sequencing and the widespread use of high-throughput analysis for identifying novel lncRNAs. Since then, the characterization of lncRNAs has contributed to the establishment of their molecular roles and functions in transcriptional regulation. Although genetic studies have so far explored the sequence-based primary function of lncRNAs that guides the expression of target genes, recent insights have shed light on the potential of lncRNAs for widening the identification of biomarkers from non-degenerative to neurodegenerative diseases. Therefore, further advances in the genetic characteristics of lncRNAs are expected to lead to diagnostic accuracy during disease progression. In this review, we summarized the latest studies of lncRNAs in TBI as a non-degenerative disease and discussed their potential limitations for clinical treatment.
Impact of Isolation Procedures on the Development of a Preclinical Synovial Fibroblasts/Macrophages in an In Vitro Model of Osteoarthritis
Authors: Manferdini Cristina , Saleh Yasmin , Dolzani Paolo , Gabusi Elena , Trucco Diego , Filardo Giuseppe , Lisignoli Gina
Published: December 2020

There is a lack ofin vitromodels able to properly represent osteoarthritis (OA) synovial tissue (ST). We aimed to characterize OA ST and to investigate whether a mechanical or enzymatic digestion procedures influence synovial cell functional heterogeneity in vitro. Procedures using mechanical nondigested fragments (NDF), synovial digested fragments (SDF), and filtrated synovial digested cells (SDC) were compared. An immunophenotypic profile was performed to distinguish synovial fibroblasts (CD55, CD73, CD90, CD106), macrophages (CD14, CD68), M1-like (CD80, CD86), and M2-like (CD163, CD206) synovial macrophages. Pro-inflammatory (interleukin 6 IL6), tumor necrosis factor alpha (TNFα), chemokine C-C motif ligand 3 (CCL3/MIP1α), C-X- motif chemokine ligand 10 (CXCL10/IP10) and anti-inflammatory (interleukin 10 (IL10)), transforming growth factor beta 1 (TGFβ1), C-C motif chemokine ligand 18 (CCL18) cytokines were evaluated. CD68 and CD163 markers were higher in NDF and SDF compared to the SDC procedure, while CD80, CD86, and CD206 were higher only in NDF compared to the SDC procedure. Synovial fibroblast markers showed similar percentages. TNFα, CCL3/MIP1α, CXCL10/IP10, and CCL18 were higher in NDF compared to SDC, but not compared to SDF. IL10 and TGFβ1 were higher in NDF than SDC at the molecular level, while IL6 did not show differences among procedures. We demonstrated that NDF isolation procedures better preserved the heterogeneity of specific OA synovial populations (fibroblasts, macrophages), fostering their use for testing new cell therapies or drugs for OA, reducing or avoiding the use of animal models.