bluuebunny/crossref_metadata_2025 huggingface dataset:
High-Throughput UV Photoionization and Fragmentation of Neutral Biomolecules as a Structural Fingerprint
Authors: Wang Siwen , Dauletyarov Yerbolat , Horke Daniel A.
Published: June 2023

We present UV photofragmentation studies of the structural isomers paracetamol, 3-Pyridinepropionic acid (3-PPIA) and (R)-(-)-2-Phenylglycine. In particular, we utilized a new laser-based thermal desorption source in combination with femtosecond multiphoton ionization at 343 nm and 257 nm. The continuous nature of our molecule source, combined with the 50 kHz repetition rate of the laser, allowed us to perform these experiments at high throughput. In particular, we present detailed laser intensity dependence studies at both wavelengths, producing 2D mass spectra with highly differential information about the underlying fragmentation processes. We show that UV photofragmentation produces highly isomer-specific mass spectra, and assign all major fragmentation pathways observed. The intensity-dependence measurements, furthermore, allowed us to evaluate the appearance intensities for each fragmentation channel, which helped to distinguish competing from consecutive fragmentation pathways.
Kinetin Capped Zinc Oxide Nanoparticles Improve Plant Growth and Ameliorate Resistivity to Polyethylene Glycol (PEG)-Induced Drought Stress in Vigna radiata (L.) R. Wilczek (Mung Bean)
Authors: Ajmal Maham , Ullah Rehman , Muhammad Zahir , Khan Muhammad Nauman , Kakar Hussain Ahmad , Kaplan Alevcan , Okla Mohammad K. , Saleh Ibrahim A. , Kamal Asif , Abdullah Abdullah , Abdul Razak Sarah
Published: June 2023

Plants are sessile and mostly exposed to various environmental stresses which hamper plant growth, development, and significantly decline its production. Drought stress is considered to be one of the most significant limiting factors for crop plants, notably in arid and semi-arid parts the world. Therefore, the present study aimed to evaluate the potential impact of different concentrations (10, 100, and 200 µg/mL) of kinetin capped zinc oxide nanoparticles (Kn-ZnONPs) on Vigna radiata (L.) R. Wilczek under varying levels (5%, 10%, 15%) of PEG-induced drought stress. ZnONPs were synthesized by a co-precipitation method using Zinc acetate as a precursor at pH-12, incinerated to 500 °C, and kinetin was used as a surface functionalizing agent. The resulting Kn-ZnONPs were characterized by various contemporary analytical techniques, including SEM, SEM-EDS, XRD, DLS, and Zeta potential and IR spectroscopy. Crystalline Kn-ZnONPs, with a zeta potential of 27.8 mV and a size of 67.78 nm, of hexagonal wurtzite structure and vibrational stretches associated with N-H, C-O, C-N, etc., were confirmed. PEG-induced drought stress significantly reduced the growth of V. radiata by declining the chlorophyll and carotenoid contents. Moreover, a significant decrease in the levels of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), soluble sugar contents, proline, protein contents, phenol, and tannin were observed compared to the control. However, the exogenous application of Kn-ZnONPs ameliorated all photosynthetic parameters by up-regulating the antioxidant defense system through the promotion of SOD, POD, CAT, and lipid peroxidation levels. The biochemical parameters, such as proteins, soluble sugars, and proline, were observed to be maximum in plants treated with 200 µg/mL Kn-ZnONPs under 5% drought stress. The application of Kn-ZnONPs also enhanced the total phenol contents, flavonoid, and tannin contents. In conclusion, the findings of this study demonstrate that the exogenous application of Kn-ZnONPs provides beneficial effects to V. radiata by attenuating the damaging effects of drought stress through the up-regulation of the antioxidant defense system and osmolytes. These results suggest that Kn-ZnONPs have potential as a novel approach to improve crop productivity under drought stress conditions.
Bragg–Williams Theory for Particles with a Size-Modulating Internal Degree of Freedom
Authors: Bossa Guilherme Volpe , May Sylvio
Published: June 2023

The field of soft matter teems with molecules and aggregates of molecules that have internal size-modulating degrees of freedom. Proteins, peptides, microgels, polymers, micelles, and even some colloids can exist in multiple—often just two dominating—states with different effective sizes, where size can refer to the volume or to the cross-sectional area for particles residing on surfaces. The size-dependence of their accessible states renders the behavior of these particles pressure-sensitive. The Bragg–Williams model is among the most simple mean-field methods to translate the presence of inter-particle interactions into an approximate phase diagram. Here, we extend the Bragg–Williams model to account for the presence of particles that are immersed in a solvent and exist in two distinct states, one occupying a smaller and the other one a larger size. The basis of the extension is a lattice–sublattice approximation that we use to host the two size-differing states. Our model includes particle–solvent interactions that act as an effective surface tension between particles and solvent and are ignorant of the state in which the particles reside. We analyze how the energetic preference of the particles for one or the other state affects the phase diagrams. The possibility of a single phase-two phases-single phase sequence of phase transitions as a function of increasing temperature is demonstrated.
The Metschnikowia pulcherrima Clade as a Model for Assessing Inhibition of Candida spp. and the Toxicity of Its Metabolite, Pulcherrimin
Authors: Kregiel Dorota , Czarnecka-Chrebelska Karolina H. , Schusterová Hana , Vadkertiová Renáta , Nowak Adriana
Published: June 2023

Candidiasis is one of the most frequent infections worldwide. In this study, the antimicrobial properties of six strains belonging to the Metschnikowia pulcherrima clade were evaluated against twenty Candida and Candida-related Filobasidiella neoformans var. bacillispora (syn. Cryptococcus neoformans) of different origins, employing the agar cross method. The toxic effect of pulcherrimin, a red metabolite that is responsible for the antimicrobial activities of Metschnikowia spp., was evaluated in various experimental models. The results of agar tests showed that the selected M. pulcherrima strains inhibited the growth of the Candida and non-Candida strains. However, inhibition was dependent on the strain and the environment. The presence of peptone, sodium silicate, and a higher incubation temperature decreased the antifungal action of the M. pulcherrima strains. Pulcherrimin showed cytotoxic and antiproliferative activity, with oxidative stress in cells leading to apoptosis. More research is needed on the mechanism of action of pulcherrimin on somatic cells.
Polymer-Based Wound Dressings Loaded with Ginsenoside Rg3
Authors: Yang Jiali , Zhang Lifeng , Peng Xiaojuan , Zhang Shuai , Sun Shuwen , Ding Qiteng , Ding Chuanbo , Liu Wencong
Published: June 2023

The skin, the largest organ in the human body, mainly plays a protective role. Once damaged, it can lead to acute or chronic wounds. Wound healing involves a series of complex physiological processes that require ideal wound dressings to promote it. The current wound dressings have characteristics such as high porosity and moderate water vapor permeability, but they are limited in antibacterial properties and cannot protect wounds from microbial infections, which can delay wound healing. In addition, several dressings contain antibiotics, which may have bad impacts on patients. Natural active substances have good biocompatibility; for example, ginsenoside Rg3 has anti-inflammatory, antibacterial, antioxidant, and other biological activities, which can effectively promote wound healing. Some researchers have developed various polymer wound dressings loaded with ginsenoside Rg3 that have good biocompatibility and can effectively promote wound healing and reduce scar formation. This article will focus on the application and mechanism of ginsenoside Rg3-loaded dressings in wounds.
Recent Progress in Crystalline Borates with Edge-Sharing BO4 Tetrahedra
Authors: Li Jing-Jing , Chen Wei-Feng , Lan You-Zhao , Cheng Jian-Wen
Published: June 2023

Crystalline borates have received great attention due to their various structures and wide applications. For a long time, the corner-sharing B–O unit is considered a basic rule in borate structural chemistry. The Dy4B6O15 synthesized under high-pressure is the first oxoborate with edge-sharing [BO4] tetrahedra, while the KZnB3O6 is the first ambient pressure borate with the edge-sharing [BO4] tetrahedra. The edge-sharing connection modes greatly enrich the structural chemistry of borates and are expected to expand new applications in the future. In this review, we summarize the recent progress in crystalline borates with edge-sharing [BO4] tetrahedra. We discuss the synthesis, fundamental building blocks, structural features, and possible applications of these edge-sharing borates. Finally, we also discuss the future perspectives in this field.
Cyclopentenylcytosine (CPE-C): In Vitro and In Vivo Evaluation as an Antiviral against Adenoviral Ocular Infections
Authors: Romanowski Eric G. , Yates Kathleen A. , Gordon Y. Jerold
Published: June 2023

Adenoviruses are the major cause of ocular viral infections worldwide. Currently, there is no approved antiviral treatment for these eye infections. Cyclopentenylcytosine (CPE-C) is an antiviral that has demonstrated activity against more than 20 viruses. The goals of the current study were to determine the in vitro and in vivo antiviral activity of CPE-C as well as its ocular toxicity. Antiviral activity was evaluated in vitro using standard plaque reduction assays to determine the 50% effective concentrations (EC50s) and in vivo in the Ad5/NZW rabbit ocular replication model. Ocular toxicity was determined in uninfected rabbit eyes following topical ocular application. The in vitro EC50s for CPE-C ranged from 0.03 to 0.059 μg/mL for nine adenovirus types that commonly infect the eye. Ocular toxicity testing determined CPE-C to be non-irritating or practically non-irritating by Draize scoring. In vivo, 3% CPE-C topically administered 4X or 2X daily for 7 days to adenovirus-infected eyes demonstrated effective antiviral activity compared with the negative control and comparable antiviral activity to the positive control, 0.5% cidofovir, topically administered twice daily for 7 days. We conclude CPE-C was relatively non-toxic to rabbit eyes and demonstrated potent anti-adenoviral activity in vitro and in vivo.
Scutellaria baicalensis Georgi and Their Natural Flavonoid Compounds in the Treatment of Ovarian Cancer: A Review
Authors: Cai Jiaying , Hu Qichao , He Zhelin , Chen Xiaoyan , Wang Jian , Yin Xiang , Ma Xiao , Zeng Jinhao
Published: June 2023

Ovarian cancer (OC) is one of the most common types of cancer in women with a high mortality rate, and the treatment of OC is prone to high recurrence rates and side effects. Scutellaria baicalensis (SB) is a herbal medicine with good anti-cancer activity, and several studies have shown that SB and its flavonoids have some anti-OC properties. This paper elucidated the common pathogenesis of OC, including cell proliferation and cell cycle regulation, cell invasion and metastasis, apoptosis and autophagy, drug resistance and angiogenesis. The mechanisms of SB and its flavonoids, wogonin, baicalein, baicalin, Oroxylin A, and scutellarein, in the treatment of OC, are revealed, such as wogonin inhibits proliferation, induces apoptosis, inhibits invasion and metastasis, and increases the cytotoxicity of the drug. Baicalein also inhibits vascular endothelial growth factor (VEGF) expression etc. Analyzing their advantages and disadvantages in treating OC provides a new perspective on the role of SB and its flavonoids in OC treatment. It serves as a resource for future OC research and development.
Size Uncertainty in Individual Nanoparticles Measured by Single Particle Inductively Coupled Plasma Mass Spectrometry
Authors: Yamashita Shuji , Miyashita Shin-ichi , Hirata Takafumi
Published: June 2023

Single particle inductively coupled plasma mass spectrometry has been used for size measurements of individual nanoparticles (NPs). Here, uncertainties in size analysis based upon two calibration approaches were evaluated: (i) the use of particle size standard and (ii) the use of ion standard solution. For particle size standard approach, the source of uncertainty to determine the target NP diameter was related to the variation in the signal intensities of both target NPs and particle size standard, and the size distribution of the particle size standard. The relative uncertainties of the 50 nm silver NP as the target were 15.0%, 9.9%, and 10.8% when particle size standards of 30 nm, 60 nm, and 100 nm silver NPs were used, respectively. As for the ion standard solution approach, the sources of uncertainty were the concentration of working standard solution, sample flow rate, transport efficiency, slope of calibration curve, and variation in the signal intensity of the ion standard solution and of the target NPs. The relative uncertainties for the 50 nm silver NP were 18.5% for 1 ng/g, 7.6% for 10 ng/g, and 4.7% for 100 ng/g solutions. The lower uncertainty obtained with a high concentration working standard solution is recommended to improve precision on particle size determinations by spICP-MS.
Atomistic to Mesoscopic Modelling of Thermophysical Properties of Graphene-Reinforced Epoxy Nanocomposites
Authors: Muhammad Atta , Sáenz Ezquerro Carlos , Srivastava Rajat , Asinari Pietro , Laspalas Manuel , Chiminelli Agustín , Fasano Matteo
Published: June 2023

This research addresses the need for a multiscale model for the determination of the thermophysical properties of nanofiller-enhanced thermoset polymer composites. Specifically, we analyzed the thermophysical properties of an epoxy resin containing bisphenol-A diglyceryl ether (DGEBA) as an epoxy monomer and dicyandiamide (DICY) and diethylene triamine (DETA) as cross-linking agents. The cross-linking process occurs at the atomistic scale through the formation of bonds among the reactive particles within the epoxy and hardener molecules. To derive the interatomic coarse-grained potential for the mesoscopic model and match the density of the material studied through atomic simulations, we employed the iterative Boltzmann inversion method. The newly developed coarse-grained molecular dynamics model effectively reproduces various thermophysical properties of the DGEBA-DICY-DETA resin system. Furthermore, we simulated nanocomposites made of the considered epoxy additivated with graphene nanofillers at the mesoscopic level and verified them against continuum approaches. Our results demonstrate that a moderate amount of nanofillers (up to 2 wt.%) increases the elastic modulus and thermal conductivity of the epoxy resin while decreasing the Poisson’s ratio. For the first time, we present a coarse-grained model of DGEBA-DICY-DETA/graphene materials, which can facilitate the design and development of composites with tunable thermophysical properties for a potentially wide range of applications, e.g., automotive, aerospace, biomedical, or energy ones.
Stability and Photothermal Properties of Fe3O4-H2O Magnetic Nanofluids
Authors: Zhang Chengya , Gao Lei , Zhou Xiaofeng , Wu Xiaohu
Published: June 2023

Solar collectors are more efficient and commercial devices for collecting solar energy, compared to other solar energy utilizations. To improve the efficiency of solar collectors, it is important to prepare a liquid heat-collecting medium, which is stable and has high photothermal properties. Therefore, in this work, we develop a droplet–droplet mixing technique to prepare Fe3O4-H2O magnetic nanofluid. The results show that magnetic nanofluids prepared using the droplet–droplet mixing technique have more stable performance and a better encapsulation of dispersants than those prepared via traditional liquid–liquid mixing. Then, the thermal conductivity and photothermal properties of Fe3O4-H2O magnetic nanofluids are investigated experimentally and theoretically. The thermal conductivity and temperature of the magnetic nanofluid with Fe3O4 nanoparticles of a 1.0% volume fraction can reach the maximum value of 0.95 W/m∙K and 73.9 °C when the magnetic field strength is equal to the saturation magnetic field of 800 Gs. These findings provide insights into the potential applications of Fe3O4-H2O magnetic nanofluids in direct absorption solar collectors, heat exchangers, automobile radiators, etc.
Developing High-Power-Density Electromagnetic Devices with Nanocrystalline and Amorphous Magnetic Materials
Authors: Guo Youguang , Liu Lin , Yin Wenliang , Lu Haiyan , Lei Gang , Zhu Jianguo
Published: June 2023

With the increasing demand for smaller, lighter, and more affordable electromagnetic devices, there is a growing trend toward developing high-power-density transformers and electrical machines. While increasing the operating frequency is a straightforward approach to achieving high power density, it can lead to significant power loss within a limited volume, resulting in excessive temperature rise and device degradation. Therefore, it is crucial to design high-power-density electromagnetic devices that exhibit low power loss and efficient thermal dissipation to address these challenges. Advanced techniques, such as the utilization of novel and advanced electromagnetic materials, hold great promise for overcoming these issues. Specifically, nanocrystalline and amorphous magnetic materials have emerged as highly effective solutions for reducing power loss and increasing efficiency in electromagnetic devices. This paper aims to provide an overview of the application of nanocrystalline and amorphous magnetic materials in transformers and electrical machines, along with key technologies and the major challenges involved.
Nonlinear Optical Modulation Characteristics of MXene Cr2C for 2 μm Pulsed Lasers
Authors: Yu Jingcheng , Chen Zijun , Li Tao , Feng Tianli , Huang Jiacheng , Liu Yizhou , Ni Zheng , Yu Li , Qiao Wenchao
Published: June 2023

MXene materials have shown numerous useful mechanical and electronic properties, and have been found to possess nice potential in the field of optical modulation. Here, we fabricated a MXene Cr2C saturable absorber by the liquid-phase exfoliation method, and systemically analyzed the surface morphology and nonlinear properties of the Cr2C sample. Applying the Cr2C saturable absorber as a Q-switch in a thulium-doped yttrium aluminum perovskite (Tm: YAP) laser, the shortest single pulse was obtained with a width of 602 ns under an absorbed pump power of 3.3 W at a repetition rate of 55 kHz with a T = 1% output coupler. The maximum output power was obtained with a T = 5% output coupler at a repetition rate of 58 kHz. The obtained maximum pulse energy and peak power were 3.96 μJ and 4.36 W, separately, which reveal that the MXene Cr2C can be applied as a promising modulation material in the near-infrared pulsed lasers.
The Occurrence of Oxidative Stress Induced by Silver Nanoparticles in Chlorella vulgaris Depends on the Surface-Stabilizing Agent
Authors: Komazec Bruno , Cvjetko Petra , Balen Biljana , Letofsky-Papst Ilse , Lyons Daniel Mark , Peharec Štefanić Petra
Published: June 2023

Silver nanoparticles (AgNPs) are of great interest due to their antimicrobial properties, but their reactivity and toxicity pose a significant risk to aquatic ecosystems. In biological systems, AgNPs tend to aggregate and dissolve, so they are often stabilized by agents that affect their physicochemical properties. In this study, microalga Chlorella vulgaris was used as a model organism to evaluate the effects of AgNPs in aquatic habitats. Algae were exposed to AgNPs stabilized with citrate and cetyltrimethylammonium bromide (CTAB) agents and to AgNO3 at concentrations that allowed 75% cell survival after 72 h. To investigate algal response, silver accumulation, ROS content, damage to biomolecules (lipids, proteins, and DNA), activity of antioxidant enzymes (APX, PPX, CAT, SOD), content of non-enzymatic antioxidants (proline and GSH), and changes in ultrastructure were analyzed. The results showed that all treatments induced oxidative stress and adversely affected algal cells. AgNO3 resulted in the fastest death of algae compared to both AgNPs, but the extent of oxidative damage and antioxidant enzymatic defense was similar to AgNP-citrate. Furthermore, AgNP-CTAB showed the least toxic effect and caused the least oxidative damage. These results highlight the importance of surface-stabilizing agents in determining the phytotoxicity of AgNPs and the underlying mechanisms affecting aquatic organisms.
Editorial for the Special Issue “Biological and Toxicological Studies of Nanoparticles”
Authors: Joubert Olivier
Published: June 2023

Nanoparticles have attracted a great deal of attention over the past two decades or more due to their unique size-dependent physical and chemical properties [...]
Effect of Void Defects on the Indentation Behavior of Ni/Ni3Al Crystal
Authors: Yang Longlong , Sun Kun , Wu Huaying
Published: June 2023

Inconel 718 (IN 718) superalloys are widely used as engineering materials owing to their superior mechanical performance. And voids are unavoidable defects in IN 718 superalloy preparation, which dramatically affect the mechanical properties of IN 718 superalloys. In this work, the effects of void radius, distance from the top of the void to the substrate surface, and substrate temperature on the mechanical properties of the Ni/Ni3Al crystal are systematically investigated. It is shown that voids affect the formation of stair-rod dislocations and Shockley dislocations in the substrate, which in turn determines the mechanical properties. Thus, with the increase in void radius, Young’s modulus and hardness gradually decrease. With the increase in void distance, Young’s modulus and hardness increase and finally tend to be stable. In addition, the increase in substrate temperature leads to the interphase boundary becoming irregular and increases the defects in the γ and γ″ phases. As a result, Young’s modulus and hardness of the substrate decrease. This work aims to provide a guideline for investigating the indentation properties of Ni-based superalloys using MD.
Tunnel Josephson Junction with Spin–Orbit/Ferromagnetic Valve
Authors: Neilo Alexey , Bakurskiy Sergey , Klenov Nikolay , Soloviev Igor , Kupriyanov Mikhail
Published: June 2023

We have theoretically studied the transport properties of the SIsNSOF structure consisting of thick (S) and thin (s) films of superconductor, an insulator layer (I), a thin film of normal metal with spin–orbit interaction (SOI) (NSO), and a monodomain ferromagnetic layer (F). The interplay between superconductivity, ferromagnetism, and spin–orbit interaction allows the critical current of this Josephson junction to be smoothly varied over a wide range by rotating the magnetization direction in the single F-layer. We have studied the amplitude of the spin valve effect and found the optimal ranges of parameters.
LncRNA Functional Screening in Organismal Development
Authors: Li Yang , Zhai Huicong , Tong Lingxiu , Wang Cuicui , Xie Zhiming , Zheng Ke
Published: June 2023

Controversy continues over the functional prevalence of long non-coding RNAs (lncRNAs) despite their being widely investigated in all kinds of cells and organisms. In animals, lncRNAs have aroused general interest from exponentially increasing transcriptomic repertoires reporting their highly tissue-specific and developmentally dynamic expression, and more importantly, from growing experimental evidence supporting their functionality in facilitating organogenesis and individual fitness. In mammalian testes, while a great multitude of lncRNA species are identified, only a minority of them have been shown to be useful, and even fewer have been demonstrated as true requirements for male fertility using knockout models to date. This noticeable gap is attributed to the virtual existence of a large number of junk lncRNAs, the lack of an ideal germline culture system, difficulty in loss-of-function interrogation, and limited screening strategies. Facing these challenges, in this review, we discuss lncRNA functionality in organismal development and especially in mouse testis, with a focus on lncRNAs with functional screening.
Prevalence of Malnutrition in People with Dementia in Long-Term Care: A Systematic Review and Meta-Analysis
Authors: Perry Emma , Walton Karen , Lambert Kelly
Published: June 2023

Dementia is a common syndrome in older people. Dementia alters eating behaviors, hunger and thirst cues, swallow function, ability to self-feed, and recognition and interest in food. There is significant variation in the reported prevalence of malnutrition among older people who live in long-term care. The aim was to conduct a systematic literature review and meta-analysis of the prevalence of malnutrition in those with dementia living in long-term care using a validated nutrition assessment tool. Scopus, Web of Science, CINAHL, and Medline were searched. A random effects model was used to determine the prevalence and risk of malnutrition. Data were retrieved from 24 studies. Most of the studies were from Europe or South Asia. The prevalence of malnutrition ranged from 6.8 to 75.6%, and the risk of malnutrition was 36.5–90.4%. The pooled prevalence of malnutrition in those with dementia in long-term care was 26.98% (95% CI 22.0–32.26, p < 0.0001, I2 = 94.12%). The pooled prevalence of the risk of malnutrition in those with dementia was 57.43% (95% CI 49.39–65.28, p < 0.0001, I2 = 97.38%). Malnutrition is widespread in those with dementia living in long-term care. Further research exploring malnutrition in other industrialized countries using validated assessment tools is required.
Association of Preoperative Prognostic Nutritional Index with Risk of Postoperative Acute Kidney Injury: A Meta-Analysis of Observational Studies
Authors: Liu Chien-Cheng , Liu Ping-Hsin , Chen Hsiao-Tien , Chen Jui-Yi , Lee Chia-Wei , Cheng Wan-Jung , Chen Jen-Yin , Hung Kuo-Chuan
Published: June 2023

This meta-analysis aimed to assess the clinical association of the preoperative prognostic nutritional index (pre-PNI) with the risk of postoperative acute kidney injury. Four databases (e.g., Medline) were searched from inception to December 2022 to investigate the association between pre-PNI (i.e., low vs. high) and PO-PNI as well as the correlation between pre-PNI and other postoperative prognostic indices. Overall, 13 observational studies, including 9185 patients, were eligible for analysis. A low PNI was related to increased risks of PO-AKI [odd ratio (OR) = 1.65, p = 0.001, 3811 patients], postoperative infection (OR = 2.1, p < 0.00001, 2291 patients), and mortality (OR = 1.93, p < 0.0001, 2159 patients). Albeit statistically nonsignificant, a trend was noted, linking a low PNI to higher risks of postoperative bleeding (OR = 2.5, p = 0.12, 1157 patients) and stroke (OR = 1.62, p = 0.07, 2036 patients). Pooled results revealed a prolonged intensive care unit (ICU) stay in patients with low PNIs compared to those with high PNIs (MD: 0.98 days, p = 0.02, 2209 patients) without a difference in hospital stay between the two groups (MD: 1.58 days, p = 0.35, 2249 patients). This meta-analysis demonstrated an inverse correlation between PNI and the risks of PO-AKI, postoperative infection, and mortality, as well as the length of ICU stay, which warrants further investigations for verification.
Exploring Ethical, Ecological, and Health Factors Influencing the Acceptance of Cultured Meat among Generation Y and Generation Z
Authors: Pilařová Lucie , Balcarová Tereza , Pilař Ladislav , Kvasničková Stanislavská Lucie , Rosak-Szyrocka Joanna , Pitrová Jana , Moulis Pavel , Kvasnička Roman
Published: June 2023

Growing research and technological development is making the commercial production of cultured meat as a sustainable alternative to livestock-derived meat an increasing reality. However, to competitively position cultured meat on the food market, appropriate marketing and communication tailored to specific demographics is required. We aimed to define the motives that influence the willingness to include cultured meat in consumption based on age, specifically in Generation Z and Generation Y. To achieve this, data from a questionnaire survey that asked about ethical, ecological and health and safety factors around cultured meat was collected from 740 respondents (301 Generation Z and 439 Generation Y) and analyzed using the Mann–Whitney test and structural equation modeling. Generation Z were significantly more likely than Generation Y (p < 0.05) to consider cultured meat healthier than conventional meat because of the possibility of adjusting the composition and nutrient content. Generation Z were also significantly less concerned than Generation Y (p < 0.05) about the consequences that consuming cultured meat might have on human health. In Generation Z, ethical, ecological and health and safety factors significantly influenced their willingness to consume cultured meat (all p < 0.01). In conclusion, we confirmed the influence of ecological and ethical awareness, as well as health and safety, on willingness to include cultured meat in consumption; these areas could be targeted when marketing cultured meat.
Association between Gut Microbiota and Digestive System Cancers: A Bidirectional Two-Sample Mendelian Randomization Study
Authors: Xie Ning , Wang Ziwei , Shu Qiuai , Liang Xiru , Wang Jinhai , Wu Kaichun , Nie Yongzhan , Shi Yongquan , Fan Daiming , Wu Jian
Published: June 2023

Accumulating evidence indicates that gut microbiota closely correlates with the tumorigenesis of digestive system cancers (DSCs). However, whether the causality between gut microbiota and DSCs exists is unknown. Genome-wide association study (GWAS) summary statistics for gut microbiota and DSCs and the bidirectional two-sample Mendelian randomization (MR) analysis were utilized to assess the causality between gut microbiota and DSCs. Sensitivity analyses were performed to evaluate the robustness of our results. We found that the genus Eggerthella (OR = 0.464, 95%CI: 0.27 to 0.796, p = 0.005) was negatively associated with the risk of gastric cancer. The genetically predicted genus Lachnospiraceae FCS020 group (OR = 0.607, 95%CI: 0.439 to 0.84, p = 0.003) correlated with a lower risk of colorectal cancer, and genus Turicibacter (OR = 0.271, 95%CI: 0.109 to 0.676, p = 0.005) was a protective factor for liver cancer. In the reverse MR, DSCs regulated the relative abundance of specific strains of gut microbiota. We comprehensively screened the association between gut microbiota and DSCs using a bidirectional two-sample MR analysis and identified the causality between several microbial taxa and DSCs. Our discoveries are beneficial for the development of novel microbial markers and microbiota-modifying therapeutics for DSC patients.
A Body Shape Index and Its Changes in Relation to All-Cause Mortality among the Chinese Elderly: A Retrospective Cohort Study
Authors: Yang Ning , Zhuo Jialu , Xie Suyi , Qu Zhihua , Li Wei , Li Zixiang , Guo Panpan , Gao Mingbo , Qin Huanlong , Han Ting
Published: June 2023

Although recent evidence has revealed that a body shape index (ABSI) is correlated with the incidence of death among different ethnicities, there remains a paucity of studies investigating the impact of ABSI on mortality within the Chinese elderly. Our objective was to ascertain the link between ABSI, as well as its alterations over time, and all-cause mortality among Chinese aged 65 y and above. A total of 3789 participants were enrolled from the Chinese Longitudinal Healthy Longevity Survey (CLHLS). Cox regressions and restricted cubic splines were employed to assess the association of ABSI and relative changes with all-cause mortality. When nonlinearity was detected, a restricted cubic spline regression was subsequently conducted to compute hazard ratios and 95% confidence intervals. The median survival time was 46 months, and 1342 individuals (35.4%) were reported to have died. ABSI contributed independently to rising death rates among Chinese old populations according to univariate and multivariate Cox regressions. Statistically significant associations were also found stratified by age, sex, and lifestyle. A U-shaped association of ABSI changes with all-cause mortality (p = 0.027) was observed, indicating that old adults with stable ABSI during the follow-up period experienced the lowest risk of mortality. After multivariable adjustment, participants with a 10% reduction in ABSI changes had an increased 9.4% risk of death, while participants with a 10% rise in ABSI changes had an increased 1.9% risk. ABSI and its changes are predictors for all-cause mortality among the elderly Chinese population, which emphasizes the clinical importance of monitoring ABSI and keeping it stable over time.
Neutrino Spectrum and Energy Loss Rates Due to Weak Processes on Hot 56Fe in Pre-Supernova Environment
Authors: Dzhioev A. A. , Yudin A. V. , Dunina-Barkovskaya N. V. , Vdovin A. I.
Published: June 2023

Applying TQRPA calculations of Gamow–Teller strength functions in hot nuclei, we compute the (anti)neutrino spectra and energy loss rates arising from weak processes on hot 56Fe under pre-supernova conditions. We use a realistic pre-supernova model calculated by the stellar evolution code MESA. Taking into account both charged and neutral current processes, we demonstrate that weak reactions with hot nuclei can produce high-energy (anti)neutrinos. We also show that, for hot nuclei, the energy loss via (anti)neutrino emission is significantly larger than that for nuclei in their ground state. It is found that the neutral current de-excitation via the νν¯-pair emission is presumably a dominant source of antineutrinos. In accordance with other studies, we confirm that the so-called single-state approximation for neutrino spectra might fail under certain pre-supernova conditions.
Role of D(−)-Lactic Acid in Prevention of Chlamydia trachomatis Infection in an In Vitro Model of HeLa Cells
Authors: Zalambani Chiara , Rizzardi Nicola , Marziali Giacomo , Foschi Claudio , Morselli Sara , Djusse Marielle Ezekielle , Naldi Marina , Fato Romana , Calonghi Natalia , Marangoni Antonella
Published: June 2023

A vaginal microbiota dominated by certain Lactobacillus species may have a protective effect against Chlamydia trachomatis infection. One of the key antimicrobial compounds produced is lactic acid, which is believed to play a central role in host defense. Lactobacillus strains producing the D(−)-lactic acid isomer are known to exert stronger protection. However, the molecular mechanisms underlying this antimicrobial action are not well understood. The aim of this study was to investigate the role of D(−)-lactic acid isomer in the prevention of C. trachomatis infection in an in vitro HeLa cell model. We selected two strains of lactobacilli belonging to different species: a vaginal isolate of Lactobacillus crispatus that releases both D(−) and L(+) isomers and a strain of Lactobacillus reuteri that produces only the L(+) isomer. Initially, we demonstrated that L. crispatus was significantly more effective than L. reuteri in reducing C. trachomatis infectivity. A different pattern of histone acetylation and lactylation was observed when HeLa cells were pretreated for 24 h with supernatants of Lactobacillus crispatus or L. reuteri, resulting in different transcription of genes such as CCND1, CDKN1A, ITAG5 and HER-1. Similarly, distinct transcription patterns were found in HeLa cells treated with 10 mM D(−)- or L(+)-lactic acid isomers. Our findings suggest that D(−) lactic acid significantly affects two non-exclusive mechanisms involved in C. trachomatis infection: regulation of the cell cycle and expression of EGFR and α5β1-integrin.
Evaluation of Genetic Diversity and Virulence Potential of Legionella pneumophila Isolated from Water Supply Systems of Residential Buildings in Latvia
Authors: Valciņa Olga , Pūle Daina , Ķibilds Juris , Labecka Linda , Terentjeva Margarita , Krūmiņa Angelika , Bērziņš Aivars
Published: June 2023

Legionella is an opportunistic pathogen with a biphasic life cycle that occasionally infects humans. The aim of the study was to assess the distribution of virulence genes and genetic diversity among L. pneumophila isolated from water supply systems of residential buildings in Latvia. In total, 492 water samples from 200 residential buildings were collected. Identification of Legionella spp. was performed according to ISO 11731, and 58 isolates were subjected to whole-genome sequencing. At least one Legionella-positive sample was found in 112 out of 200 apartment buildings (56.0%). The study revealed extensive sequence-type diversity, where 58 L. pneumophila isolates fell into 36 different sequence types. A total of 420 virulence genes were identified, of which 260 genes were found in all sequenced L. pneumophila isolates. The virulence genes enhC, htpB, omp28, and mip were detected in all isolates, suggesting that adhesion, attachment, and entry into host cells are enabled for all isolates. The relative frequency of virulence genes among L. pneumophila isolates was high. The high prevalence, extensive genetic diversity, and the wide range of virulence genes indicated that the virulence potential of environmental Legionella is high, and proper risk management is of key importance to public health.
Effect of Mannan Oligosaccharides Extracts in Uropathogenic Escherichia coli Adhesion in Human Bladder Cells
Authors: Faustino Margarida , Silva Sara , Costa Eduardo M. , Pereira Ana Margarida , Pereira Joana Odila , Oliveira Ana Sofia , Ferreira Carlos M. H. , Pereira Carla F. , Durão Joana , Pintado Manuela E. , Carvalho Ana P.
Published: June 2023

Urinary tract infections (UTIs) are a common public health problem, mainly caused by uropathogenic Escherichia coli (UPEC). Patients with chronic UTIs are usually treated with long-acting prophylactic antibiotics, which promotes the development of antibiotic-resistant UPEC strains and may complicate their long-term management. D-mannose and extracts rich in D-mannose such as mannan oligosaccharides (MOS; D-mannose oligomers) are promising alternatives to antibiotic prophylaxis due to their ability to inhibit bacterial adhesion to urothelial cells and, therefore, infection. This highlights the therapeutic potential and commercial value of using them as health supplements. Studies on the effect of MOS in UTIs are, however, scarce. Aiming to evaluate the potential benefits of using MOS extracts in UTIs prophylaxis, their ability to inhibit the adhesion of UPEC to urothelial cells and its mechanism of action were assessed. Additionally, the expression levels of the pro-inflammatory marker interleukin 6 (IL-6) were also evaluated. After characterizing their cytotoxic profiles, the preliminary results indicated that MOS extracts have potential to be used for the handling of UTIs and demonstrated that the mechanism through which they inhibit bacterial adhesion is through the competitive inhibition of FimH adhesins through the action of mannose, validated by a bacterial growth impact assessment.
Localized Drug Delivery Systems: An Update on Treatment Options for Head and Neck Squamous Cell Carcinomas
Authors: Hariharan Arvind , Tran Simon D.
Published: June 2023

Head and neck squamous cell carcinoma (HNSCC) is one of the most common cancers in the world, with surgery, radiotherapy, chemotherapy, and immunotherapy being the primary treatment modalities. The treatment for HNSCC has evolved over time, due to which the prognosis has improved drastically. Despite the varied treatment options, major challenges persist. HNSCC chemotherapeutic and immunotherapeutic drugs are usually administered systemically, which could affect the patient’s quality of life due to the associated side effects. Moreover, the systemic administration of salivary stimulating agents for the treatment of radiation-induced xerostomia is associated with toxicities. Localized drug delivery systems (LDDS) are gaining importance, as they have the potential to provide non-invasive, patient-friendly alternatives to cancer therapy with reduced dose-limiting toxicities. LDDSs involve directly delivering a drug to the tissue or organ affected by the disease. Some of the common localized routes of administration include the transdermal and transmucosal drug delivery system (DDSs). This review will attempt to explore the different treatment options using LDDSs for the treatment of HNSCC and radiotherapy-induced damage and their potential to provide a better experience for patients, as well as the obstacles that need to be addressed to render them successful.
The Exploitation of Lysosomes in Cancer Therapy with Graphene-Based Nanomaterials
Authors: Ristic Biljana , Bosnjak Mihajlo , Misirkic Marjanovic Maja , Stevanovic Danijela , Janjetovic Kristina , Harhaji-Trajkovic Ljubica
Published: June 2023

Graphene-based nanomaterials (GNMs), including graphene, graphene oxide, reduced graphene oxide, and graphene quantum dots, may have direct anticancer activity or be used as nanocarriers for antitumor drugs. GNMs usually enter tumor cells by endocytosis and can accumulate in lysosomes. This accumulation prevents drugs bound to GNMs from reaching their targets, suppressing their anticancer effects. A number of chemical modifications are made to GNMs to facilitate the separation of anticancer drugs from GNMs at low lysosomal pH and to enable the lysosomal escape of drugs. Lysosomal escape may be associated with oxidative stress, permeabilization of the unstable membrane of cancer cell lysosomes, release of lysosomal enzymes into the cytoplasm, and cell death. GNMs can prevent or stimulate tumor cell death by inducing protective autophagy or suppressing autolysosomal degradation, respectively. Furthermore, because GNMs prevent bound fluorescent agents from emitting light, their separation in lysosomes may enable tumor cell identification and therapy monitoring. In this review, we explain how the characteristics of the lysosomal microenvironment and the unique features of tumor cell lysosomes can be exploited for GNM-based cancer therapy.
Performance Testing of a Homemade Aerosol Generator for Pulmonary Administration of Dry Powder Formulations to Mice
Authors: Heida Rick , Hagedoorn Paul , van Meel Melle C. , Prins Jurrie E. R. , Simonis Frederike S. , Akkerman Renate , Huckriede Anke L. W. , Frijlink Henderik W. , de Boer Anne H. , Hinrichs Wouter L. J.
Published: June 2023

A challenge in the development of dry powder formulations for inhalation is the poor reproducibility of their administration to small laboratory animals. The currently used devices for the pulmonary administration of dry powder formulations to small rodents often function sub-optimally as they use the same puff of air for both powder dispersion and aerosol delivery. As a result, either the air volume and flow rate are too low for complete powder deagglomeration or they are too high for effective aerosol delivery to the lungs of the animal. Therefore, novel and better devices are desired. We here present an aerosol generator designed to administer a pre-generated aerosol to the lungs of mice. By mapping the complex relationship between the airflow rate, delivery time and emitted dose, we were able to control the amount of powder being delivered from the aerosol generator. The emitted aerosol had a size range favorable for lung deposition and could be measured reproducibly. Nevertheless, in vivo fluorescent imaging still revealed considerable differences between the mice in terms of the dose deposited and the distribution of powder over the lungs, suggesting that a certain biological variation in lung deposition is inevitable.
Investigation of the Process of Evolution of Traces of Explosives Carried by Fingerprints Using Polarimetric Macrophotography and Remote LF/LIF Method
Authors: Bobrovnikov Sergei , Gorlov Evgeny , Zharkov Viktor , Murashko Sergei , Vorozhtsov Alexander , Stykon Alexander
Published: June 2023

The results of a study of the degradation of the cyclotrimethylenetrinitramine (RDX) traces carried in fingerprints depending on the fingerprint number are presented. The surface concentration of the trace was assessed using macrophotography in polarized light and by the method of laser fragmentation/laser-induced fluorescence. A technique for estimating the surface concentration of RDX traces in sweat-fat fingerprints based on pixel-by-pixel scanning of macrophotographs is described. The data of parallel experiments on remote laser detection of RDX particles in fingerprints are presented. A comparison shows that the results of the direct measurements of the total trace area are in good agreement with the LF/LIF response data.
Multifrequency Vector Mm-Wave Signal Generation with No Optical Filtering Based on One Dual-Arm MZM with Phase Factor Optimization
Authors: Chen Long , Yu Qian , Liu Jiajun
Published: June 2023

In order to reduce the number of devices in and cost of a multifrequency vector millimeter-wave (mm-wave) signal generation system, we propose a novel scheme for multifrequency vector mm-wave signal generation based on one dual-arm Mach-Zehnder modulator (MZM) without optical filtering. This scheme utilizes the carrier suppression modulation of a single modulator, without the need for optical filtering and combines precoding technology to generate high-frequency QPSK and 16 QAM mm-wave signals. The transmitter is applied with precoding technology to make the frequency multiplying vector mm-wave signal display regular vector modulation at the receiver, and the system is optimized with a phase factor to ensure that the phase distribution of QPSK signal at the receiver is symmetrical. We demonstrated the generation of 4-Gbaud 76 GHz QPSK and 16 QAM vector mm-wave signals through simulation. The simulation results show that, at a fiber transmission distance of 12 km, when the launched optical power of the PD is greater than −20.535 dBm, the bit error ratio (BER) of the QPSK signal is lower than the hard-decision forward error correction (HD-FEC) threshold of 3.8 × 10−3. At a fiber transmission distance of 10 km, when the launched optical power of the PD is greater than −18.637 dBm, the BER of 16 QAM signal is also lower than 3.8 × 10−3.