bluuebunny/crossref_metadata_2025 huggingface dataset:
Pseudotachylyte-Mylonites Record of Transient Creep From Inter-Seismic Ductile to Co-Seismic Rupture
Authors: Dai Wenhao , Zhou Yongsheng , Ma Xi
Published: June 2022

Transient creep during an earthquake cycle is very important to understand the rheology of fault and deformation mechanisms in the brittle–plastic transition zone. In this paper, we analyzed the microstructures of samples for mylonites, pseudotachylyte, and cataclasite under optical microscope, SEM, and EBSD, which were collected from the Red River fault in southwest of China, where we uncovered a series of ductile to brittle deformed rocks which recorded transient creep related to earthquakes. The results show that mylonites formed at the inter-seismic creep were overprinted by pseudotachylyte and cataclasite which were produced during co-seismic rupture, and cracks in cataclasite were healed during the post-seismic relaxation. Based on the analysis of the microstructures and deformation mechanism of fault rocks, we propose the oscillation deformation model to explain transient creep of the brittle–plastic transition zone during the seismic cycle in the Red River fault.
Study on the Mechanism of Weakening Thick and Hard Roof by Deep-Hole Blasting in Deep Coal Mines
Authors: Zhang Chen , Zou Junpeng , Zhang Xiufeng , Wang Chao , Jiao Yu-Yong
Published: June 2022

In the mining process of deep coal mines with a thick and hard roof, it is easy to form a large overhanging roof and accumulate a lot of strain energy. The sudden breaking of the suspended roof may induce seisms, which seriously threaten the safety of underground miners and equipment. To reveal the mechanism of weakening the thick and hard roof by blasting in deep mining, the four times deep-hole blasting in 63upper06 panel of Dongtan coal mine are considered. First, the temporal and spatial distribution of seismicity events monitored before and after blasting is investigated. Second, the process of deep-hole blasting is simulated using finite element and discrete element methods. After blasting, the small-energy mining-induced seismicities within 20 m of the roof suddenly increase and gradually transfer to the mined-out area and the front of the working face. Meanwhile, the high rock stratum is broken and a large amount of strain energy is released, which causes the range of fall zone enlarge a lot, and the stress in the roof is decreased obviously. With the increase in the number of deep-hole blasting, the pressure relief effect becomes more obvious, and the newly developed fracture is mainly located in the low strata. Microseismic monitoring results agree with the calculated results. Moreover, the rock caving steps are easily formed after deep-hole blasting, and the collapse is more sufficient. Furthermore, blasting cracks are most restrained in the vertical direction of the maximum principal stress. Moreover, the delayed blasting method is more conducive to the expansion and penetration of blasting cracks. Results show that the deep-hole blasting technology has a significant effect on weakening the roof of coal seam and preventing and controlling large-energy mining-induced seismicities.
Mitchell–Riley Syndrome: Improving Clinical Outcomes and Searching for Functional Impact of RFX-6 Mutations
Authors: Passone Caroline de Gouveia Buff , Vermillac Gaëlle , Staels Willem , Besancon Alix , Kariyawasam Dulanjalee , Godot Cécile , Lambe Cécile , Talbotec Cécile , Girard Muriel , Chardot Christophe , Berteloot Laureline , Hachem Taymme , Lapillonne Alexandre , Poidvin Amélie , Storey Caroline , Neve Mathieu , Stan Cosmina , Dugelay Emmanuelle , Fauret-Amsellem Anne-Laure , Capri Yline , Cavé Hélène , Ybarra Marina , Chandra Vikash , Scharfmann Raphaël , Bismuth Elise , Polak Michel , Carel Jean Claude , Pigneur Bénédicte , Beltrand Jacques
Published: June 2022

Aims/Hypothesis

Caused by biallelic mutations of the gene encoding the transcription factor RFX6, the rare Mitchell–Riley syndrome (MRS) comprises neonatal diabetes, pancreatic hypoplasia, gallbladder agenesis or hypoplasia, duodenal atresia, and severe chronic diarrhea. So far, sixteen cases have been reported, all with a poor prognosis. This study discusses the multidisciplinary intensive clinical management of 4 new cases of MRS that survived over the first 2 years of life. Moreover, it demonstrates how the mutations impair the RFX6 function.

Methods

Clinical records were analyzed and described in detail. The functional impact of two RFX6R181W and RFX6V506G variants was assessed by measuring their ability to transactivate insulin transcription and genes that encode the L-type calcium channels required for normal pancreatic beta-cell function.

Results

All four patients were small for gestational age (SGA) and prenatally diagnosed with duodenal atresia. They presented with neonatal diabetes early in life and were treated with intravenous insulin therapy before switching to subcutaneous insulin pump therapy. All patients faced recurrent hypoglycemic episodes, exacerbated when parenteral nutrition (PN) was disconnected. A sensor-augmented insulin pump therapy with a predictive low-glucose suspension system was installed with good results. One patient had a homozygous c.1517T>G (p.Val506Gly) mutation, two patients had a homozygous p.Arg181Trp mutation, and one patient presented with new compound heterozygosity. The RFX6V506G and RFX6R181W mutations failed to transactivate the expression of insulin and genes that encode L-type calcium channel subunits required for normal pancreatic beta-cell function.

Conclusions/Interpretation

Multidisciplinary and intensive disease management improved the clinical outcomes in four patients with MRS, including adjustment of parenteral/oral nutrition progression and advanced diabetes technologies. A better understanding of RFX6 function, in both intestine and pancreas cells, may break ground in new therapies, particularly regarding the use of drugs that modulate the enteroendocrine system.
The Lifestyle Modifications and Endometrial Proteome Changes of Women With Polycystic Ovary Syndrome and Obesity
Authors: Abdulkhalikova D. , Sustarsic A. , Vrtačnik Bokal Eda , Jancar N. , Jensterle M. , Burnik Papler T.
Published: June 2022

Polycystic ovary syndrome (PCOS) is a polyendocrine disorder and the most common endocrinopathy in women of reproductive age. Affected women have an elevated prevalence of being overweight and obese. Our study sought to determine how weight loss associated with lifestyle changes affects the endometrium specific proteome, endocrine-metabolic characteristics, and motor capabilities of obese women with PCOS and infertility. A group of 12 infertile women under the age of 38 with PCOS and BMI ≥30 kg/m2were included in the study. An evaluation was performed by a gynecologist and an endocrinologist. The weight-loss program lasted 8 weeks under the guidance of a professional trainer. Endometrial sampling during a period of implantation window for proteome determination was performed before and after weight loss. In endometrial samples at the end of the study increased protein abundance was recorded for Legumain, Insulin-like growth factor-binding protein 7, Hepatocyte growth factor receptor, Keratin, type II cytoskeletal 7, and Cystatin-B, while the B-lymphocyte antigen CD20 protein abundance decreased. Our results also indicate significantly lowered fasting blood glucose level and free testosterone concentration and significant improvements in body composition and physical capacity. This study may open up the venues for investigating important biomarkers that may affect endometrial receptivity.

Clinical Trial Registration

https://clinicaltrials.gov/ct2/show/NCT04989244?term=NCT04989244&draw=2&rank=1, identifier: NCT04989244.
Specific miRNAs Change After 3 Months of GH treatment and Contribute to Explain the Growth Response After 12 Months
Authors: Catellani Cecilia , Ravegnini Gloria , Sartori Chiara , Righi Beatrice , Lazzeroni Pietro , Bonvicini Laura , Poluzzi Silvia , Cirillo Francesca , Predieri Barbara , Iughetti Lorenzo , Giorgi Rossi Paolo , Angelini Sabrina , Street Maria Elisabeth
Published: June 2022

Context

There is growing evidence of the role of epigenetic regulation of growth, and miRNAs potentially play a role.

Objective

The aim of this study is to identify changes in circulating miRNAs following GH treatment in subjects with isolated idiopathic GH deficiency (IIGHD) after the first 3 months of treatment, and verify whether these early changes can predict growth response.

Design and Methods

The expression profiles of 384 miRNAs were analyzed in serum in 10 prepubertal patients with IIGHD (5 M, 5 F) at two time points before starting GH treatment (t−3, t0), and at 3 months on treatment (t+3). MiRNAs with a fold change (FC) >+1.5 or <-1.5 at t+3 were considered as differentially expressed. In silico analysis of target genes and pathways led to a validation step on 8 miRNAs in 25 patients. Clinical and biochemical parameters were collected at baseline, and at 6 and 12 months. Simple linear regression analysis and multiple stepwise linear regression models were used to explain the growth response.

Results

Sixteen miRNAs were upregulated and 2 were downregulated at t+3 months. MiR-199a-5p (p = 0.020), miR-335-5p (p = 0.001), and miR-494-3p (p = 0.026) were confirmed to be upregulated at t+3. Changes were independent of GH peak values at testing, and levels stabilized after 12 months. The predicted growth response at 12 months was considerably improved compared with models using the common clinical and biochemical parameters.

Conclusions

MiR-199a-5p, miR-335-5p, and miR-494-3p changed after 3 months of GH treatment and likely reflected both the degree of GH deficiency and the sensitivity to treatment. Furthermore, they were of considerable importance to predict growth response.
Insight Into Mouse Models of Hyperthyroidism
Authors: Zhang Mengyu , Jiang Wen , Lu Ganghua , Wang Ru , Lv Zhongwei , Li Dan
Published: June 2022

Hyperthyroidism is characterized by an increase in the synthesis and secretion of thyroid hormones in the thyroid gland, and the most common cause of overproduction of thyroid hormones is Graves’ disease (GD). Long-term disease models of hyperthyroidism have been established. In general, methods to induce GD include transfection of fibroblasts, injecting plasmids or adenovirus containing thyroid stimulating hormone receptor (TSHR) or TSHR subunit, and exogenous artificial thyroid hormone supplementation. Fortunately, in mouse studies, novel treatments for GD and Graves’ orbitopathy (GO) were discovered. It has been reported that prophylactic administration of TSHR A subunit protein in genetically susceptible individuals could induce immune tolerance and provide protection for the future development of GD. Biologically active monoclonal antibody against intracellular adhesion molecule-1 (ICAM-1 mAb) and siRNA targeting TSHR can also be used to treat GD. Moreover, new potential therapeutic targets have been identified in GO mouse models, and these targets could present novel therapeutic approaches. Besides, human placental mesenchymal stem cells (hPMSCs) into the orbit, fucoxanthin and icariin may be new alternative therapies that could be used in addition to the existing drugs, although further research is needed.
Chemical Analysis and Electrochemical Monitoring of Extremely Low-Concentration Corrosive Impurity MgOHCl in Molten MgCl2–KCl–NaCl
Authors: Gong Qing , Ding Wenjin , Chai Yan , Bonk Alexander , Steinbrecher Julian , Bauer Thomas
Published: June 2022

MgCl2–KCl–NaCl is a promising thermal energy storage (TES) material and heat transfer fluid (HTF) with high operating temperatures of >700°C for next-generation concentrating solar power (CSP) plants. One major challenge for future implementation of the molten chloride TES/HTF technology arises from the presence of some corrosive impurities, especially MgOHCl, a hydrolysis product of hydrated MgCl2. Even extremely low-concentration MgOHCl (tens of ppm O in weight) can cause unneglectable corrosion of commercial Fe–Cr–Ni alloys, which limits their service time as the structural materials in the molten chloride TES/HTF system. Thus, the chemical analysis and monitoring techniques of MgOHCl at the tens of ppm O level are vital for corrosion control. In this work, a chemical analysis technique based on direct titration and a high-precision automatic titrator was developed for an exact measurement of MgOHCl at the tens of ppm O level. It shows a standard deviation below 5 ppm O and an average error below 7 ppm O when the concentration of MgOHCl is 36 ppm O. Moreover, compared to other methods available in some literature reports, it can exclude the influence of co-existing MgO on the MgOHCl concentration measurement. This chemical analysis technique was used to calibrate the previously developed electrochemical method based on cyclic voltammetry (CV) to achieve reliable in situ monitoring of MgOHCl in the MgCl2–KCl–NaCl molten salt at a concentration as low as the tens of ppm O level. The in situ monitoring technique shows a monitoring limitation of <39 ppm O. The two techniques for MgOHCl measurement developed in this work could be used to develop an in situ corrosion control system to ensure the long service time of the molten chloride TES/HTF system in next-generation CSP plants.
A Possibilistic Risk Assessment Framework for Unmanned Electric Vehicles With Predict of Uncertainty Traffic
Authors: hu wen , kang longyun , yu zongguang
Published: June 2022

At present, electric vehicles (EV) have entered a stage of rapid development. Meanwhile, with artificial intelligence (AI) technology fast improving and implementing many inventions in electric vehicles (EV), almost all EV sold in China are equipped with automatic driving technology to achieve safer and more energy-saving driving. In order to solve the problem of anti-collision in self-driving Smart EV under complex traffic, especially at intersections, most of the existing methods make sequential predictions for the driving level of vehicles, and it becomes difficult to deal with sudden changes in intentions of other vehicles. Therefore, a collision risk assessment framework based on other vehicles’ trajectory prediction is proposed. The framework integrates the solutions of other vehicles’ expected path planning, uncertainty description of driving process, trajectory change caused by obstacle intrusion, etc., as well as adopts the Gaussian mixture model to evaluate the risk according to the probability of collision. It realizes the real-time evaluation of the probability of collision and makes safe decisions and trajectory planning of the vehicles. After simulation and verification, it effectively solves the decision-making planning problem of autonomous vehicles under complicated traffic flow and demonstrates that the method is better than the current sequential prediction method (SORT\Karlman filter, etc.).
Ubiquitous Power Internet of Things-Oriented Low-Latency Edge Task Scheduling Optimization Strategy
Authors: Liang Yu , Li Taoshen
Published: June 2022

Internet of things of cloud computing offers high-performance computing, storage and networking services, but there are still suffers from a high transmission and processing latency, poor scalability and other problems. Internet of things of edge computing can better meet the increasing requirements of electricity consumers for service quality, especially the increasingly stringent need for low delay. On the other hand, edge intelligent network technology can offers edge smart sensing while significantly improve the efficiency of task execution, but it will lead to a massive collaborative task scheduling optimization problem. In order to solve this problem, This paper studies an ubiquitous power internet of things (UPIoT) smart sensing network edge computing model and an improved multi node cluster cooperative scheduling optimization strategy. The cluster server is added to the edge aware computing network, and an improved low delay edge task collaborative scheduling algorithm (LLETCS) is designed by using the vertical cooperation and multi node cluster collaborative computing scheme between edge aware networks. Then the problem is transformed based on linear reconstruction technology, and a parallel optimization framework for solving the problem is proposed. The simulation results suggest that the proposed scheme can more effectively reduce the UPIoT edge computing latency, and improve the quality of service in UPIoT smart sensing networks.
A Monte Carlo Method for Quantifying Uncertainties in the Official Greenhouse Gas Emission Factors Database of Costa Rica
Authors: Molina-Castro Gabriel
Published: June 2022

With the publication of the latest version of ISO 14064-1, the National Carbon Neutrality Program of Costa Rica included measurement uncertainty as a mandatory requirement for the reporting of greenhouse gas (GHG) inventories as an essential parameter to have precise and reliable results. However, technical gaps remain for an optimal implementation of this requirement, including a lack of information regarding uncertainties in the official database of Costa Rican emission factors. The present article sought to fill the gap of uncertainty information for 22 emission factors from this database, providing uncertainty values through the collection of input information, use of expert criteria, fitting of probability distributions, and the application of the Monte Carlo simulation method. Emission factors were classified into three groups according to their estimation methods and their information sources. Five probability distributions were chosen and fitted to the input data based on their previous application in the field. Standard uncertainties and 95% confidence intervals were estimated for each emission factor as the standard deviations and differences between the 2.5% and 97.5% percentiles of their simulated data. As expected, most of the standard uncertainties were estimated between 15% and 50% of the value of the emission factor, and confidence intervals tended to asymmetry as the standard uncertainties or the number of input data for the emission factor estimation increased. High consistency was found between these results and values reported in other studies. These results are critical to complement the official database of Costa Rican emission factors and for national users to estimate the uncertainties of their greenhouse gas inventories, easing to comply with national environmental policies by adapting to international requirements in the fight against climate change. Additionally, improvement opportunities were identified to update the emission factors from livestock enteric fermentation, manure management, waste treatments, and non-energy use of lubricants, whose estimations are based on outdated references and methodologies. An opportunity to improve and reduce the remarkably high uncertainties for emission factors associated with the biological treatment of solid waste through studies adapted to the specific characteristics of tropical countries like Costa Rica was also pointed out.
The Role of Invasion Status and Taxon of Basibionts in Marine Community Structure
Authors: Lazzeri Kate , Auker Linda A.
Published: June 2022

Studies on non-native epibionts typically focus on the organismal-level impacts of epibiosis on basibionts, rather than community-level impacts of this relationship. The purpose of our study was to evaluate if non-native basibionts in general facilitate invasions through epibiosis in Maine compared to native basibiont species. We collected 64 basibiont assemblages including replicate samples of 10 different basibiont taxa on the central Maine coast in October 2019. Each basibiont and associated epibionts were identified to genus, classified as native or non-native to the region where they were collected, and weighed. We found that while there was no association between invasion status of the epibiont and the basibiont, native basibionts had a significantly higher Shannon Diversity Index than non-native basibionts. Although diversity of epibionts was greater on native basibionts, the percentage of invaders varied across basibiont taxa. Specific basibiont taxon characteristics may be more important than status because different taxa have different surface topographies, resulting in varying settlement among epibiont species. Our study indicates that there is differential settlement of epibiont taxa across basibiont taxa, which may help predict, based on surface characteristics, which species support more epibiont taxa. This study, as a snapshot of floating dock fouling communities within a 10 km radius, may indicate that non-native basibionts play a role in changing community structure. Expanding the scope of this initial study to include a wider taxonomic and geographic range should help determine if epibiosis is truly a facilitative process in invasions.
Application of Omics Tools in Designing and Monitoring Marine Protected Areas For a Sustainable Blue Economy
Authors: Jeffery Nicholas W. , Lehnert Sarah J. , Kess Tony , Layton Kara K. S. , Wringe Brendan F. , Stanley Ryan R.E.
Published: June 2022

A key component of the global blue economy strategy is the sustainable extraction of marine resources and conservation of marine environments through networks of marine protected areas (MPAs). Connectivity and representativity are essential factors that underlie successful implementation of MPA networks, which can safeguard biological diversity and ecosystem function, and ultimately support the blue economy strategy by balancing ocean use with conservation. New “big data” omics approaches, including genomics and transcriptomics, are becoming essential tools for the development and maintenance of MPA networks. Current molecular omics techniques, including population-scale genome sequencing, have direct applications for assessing population connectivity and for evaluating how genetic variation is represented within and among MPAs. Effective baseline characterization and long-term, scalable, and comprehensive monitoring are essential for successful MPA management, and omics approaches hold great promise to characterize the full range of marine life, spanning the microbiome to megafauna across a range of environmental conditions (shallow sea to the deep ocean). Omics tools, such as eDNA metabarcoding can provide a cost-effective basis for biodiversity monitoring in large and remote conservation areas. Here we provide an overview of current omics applications for conservation planning and monitoring, with a focus on metabarcoding, metagenomics, and population genomics. Emerging approaches, including whole-genome sequencing, characterization of genomic architecture, epigenomics, and genomic vulnerability to climate change are also reviewed. We demonstrate that the operationalization of omics tools can enhance the design, monitoring, and management of MPAs and thus will play an important role in a modern and comprehensive blue economy strategy.
Causal Inference of Genetic Variants and Genes in Amyotrophic Lateral Sclerosis
Authors: Pan Siyu , Liu Xinxuan , Liu Tianzi , Zhao Zhongming , Dai Yulin , Wang Yin-Ying , Jia Peilin , Liu Fan
Published: June 2022

Amyotrophic lateral sclerosis (ALS) is a fatal progressive multisystem disorder with limited therapeutic options. Although genome-wide association studies (GWASs) have revealed multiple ALS susceptibility loci, the exact identities of causal variants, genes, cell types, tissues, and their functional roles in the development of ALS remain largely unknown. Here, we reported a comprehensive post-GWAS analysis of the recent large ALS GWAS (n = 80,610), including functional mapping and annotation (FUMA), transcriptome-wide association study (TWAS), colocalization (COLOC), and summary data-based Mendelian randomization analyses (SMR) in extensive multi-omics datasets. Gene property analysis highlighted inhibitory neuron 6, oligodendrocytes, and GABAergic neurons (Gad1/Gad2) as functional cell types of ALS and confirmed cerebellum and cerebellar hemisphere as functional tissues of ALS. Functional annotation detected the presence of multiple deleterious variants at three loci (9p21.2, 12q13.3, and 12q14.2) and highlighted a list of SNPs that are potentially functional. TWAS, COLOC, and SMR identified 43 genes at 24 loci, including 23 novel genes and 10 novel loci, showing significant evidence of causality. Integrating multiple lines of evidence, we further proposed that rs2453555 at 9p21.2 and rs229243 at 14q12 functionally contribute to the development of ALS by regulating the expression of C9orf72 in pituitary and SCFD1 in skeletal muscle, respectively. Together, these results advance our understanding of the biological etiology of ALS, feed into new therapies, and provide a guide for subsequent functional experiments.
Fear Stress During Pregnancy Affects Placental m6A-Modifying Enzyme Expression and Epigenetic Modification Levels
Authors: Wang Qiyang , Pan Mingmin , Zhang Tong , Jiang Yu , Zhao Peiyuan , Liu Xihong , Gao Anqi , Yang Liping , Hou Junlin
Published: June 2022

As the hub connecting mother and offspring, the placenta’s normal development is vital for fetal growth. Fear stress can cause some structural alterations in the placenta and affect placental development and function. N6-methyladenosine (m6A) is the most common mRNA modification and is involved in regulating the development of the placenta and embryo. There are no reports on the potential role of m6A modification in placental damage caused by fear stress during pregnancy. In this study, we demonstrated that fear stress during pregnancy increases the levels of methylated enzymes (METTL3, METTL14, and WTAP), decreases the levels of demethylase FTO, and increases the overall methylation levels in the placenta of pregnant rats. MeRIP-seq data analysis revealed 22,010 m6A peaks associated with 12,219 genes in the placenta of the model and 21,060 m6A peaks associated with 11,730 genes in the placenta of the control. The peaks were mainly concentrated in the coding region and the 3ʹ untranslated region. In addition, 50 genes with abnormal modification and expression (double aberrant genes) were screened out by combining MeRIP-seq and RNA-seq data. Mefv, Erbb2, and Cgas were selected from 50 double aberrant genes, and MeRIP-qPCR and real-time quantitative polymerase chain reaction were used to verify their modification and expression levels. Our findings suggest that m6A modifications play an important role in placental dysfunction induced by fear stress during pregnancy.
Over-Expression of GUSB Leads to Primary Resistance of Anti-PD1 Therapy in Hepatocellular Carcinoma
Authors: Kong Xiangyi , Zheng Zhiying , Song Guoxin , Zhang Zihao , Liu Hanyuan , Kang Junwei , Sun Guoqiang , Sun Guangshun , Huang Tian , Li Xiao , Rong Dawei , Wang Ke , Tang Weiwei , Xia Yongxiang
Published: June 2022

Immunotherapy treatments, particularly immune checkpoint blockade, can result in benefits in clinical settings. But many pre-clinical and clinical studies have shown that resistance to anti-PD1 therapy frequently occurs, leading to tumor recurrence and treatment failure, including in patients with hepatocellular carcinoma (HCC). In this study, 10 patients with HCC were remedied with anti-PD1, and pre-treatment biopsy samples were sequenced for 289 nanostring panel RNA to compare responsive and non-responsive tumors to identify possible pretreatment biomarkers or targets of anti-PD1 therapeutic responses. Fortunately, the expression of β-Glucuronidase (GUSB) in the non-responding tumors was found to be remarkably higher than that in responding tumors. Results of the cell counting kit 8 (CCK8), 5-ethynyl-2’-deoxyuridine (EdU), transwell, wound healing test, and flow cytometry showed that GUSB facilitated proliferation, invasion, as well as migration of human HCC cells and downregulated PD-L1 expression by promoting miR-513a-5p. Additionally, as a GUSB inhibitor, amoxapine can reduce the progression of human HCC cells, and was an effective treatment for HCC and improved the sensitivity of anti-PD1 therapy. In summary, this study reveals that increased GUSB downregulates PD-L1 expression by promoting miR-513a-5p, leading to primary resistance to anti-PD1 treatment in HCC, and amoxapine enhances the sensitivity of anti-PD1 therapy by inhibiting GUSB, providing a new strategy and method for improving the efficacy of anti-PD1 therapy and bringing new prospects for therapy of HCC.
Supplementation of Dietary Crude Lentinan Improves the Intestinal Microbiota and Immune Barrier in Rainbow Trout (Oncorhynchus mykiss) Infected by Infectious Hematopoietic Necrosis Virus
Authors: Ren Guangming , Xu Liming , Zhao Jingzhuang , Shao Yizhi , Chen Xiaoyu , Lu Tongyan , Zhang Qiya
Published: June 2022

The effects of crude lentinan (CLNT) on the intestinal microbiota and the immune barrier were evaluated in rainbow trout (Oncorhynchus mykiss) infected by infectious hematopoietic necrosis virus (IHNV). The results showed that supplementary CLNT declined the rainbow trout mortality caused by IHNV, which suggested that CLNT has preventive effects on IHNV infection. IHNV destroyed intestinal integrity, as well as caused the intestinal oxidative and damage in rainbow trout. Supplementary CLNT significantly strengthened the intestinal immune barrier by declining intestinal permeability, as well as enhancing intestinal antioxidant and anti-inflammatory abilities in IHNV-infected rainbow trout (P<0.05). In addition, CLNT modified the aberrant changes of intestinal microbiota induced by IHNV, mainly represented by promoting the growths of Carnobacterium and Deefgea and inhibiting Mycobacterium and Nannocystis. Especially, supplementing with CLNT significantly promoted the growth of short-chain fatty acid–producing bacteria (P<0.05) and consequently increased the production of acetic acid, butanoic acid, and hexanoic acid in the intestine of IHNV-infected rainbow trout. Furthermore, it was speculated that CLNT could regulate the self-serving metabolic pathways of intestinal microbiota induced by IHNV, such as fatty acid metabolism and amino acid metabolism. Together, CLNT played the antiviral effects on IHNV infection through strengthening the intestinal immune barrier, as well as regulating intestinal microbiota and SCFA metabolism in rainbow trout. The present data revealed that CLNT exerted a promising prebiotic role in preventing the rainbow trout from IHNV infection.
3D Genome Organization as an Epigenetic Determinant of Transcription Regulation in T Cells
Authors: Papadogkonas George , Papamatheakis Dionysios-Alexandros , Spilianakis Charalampos
Published: June 2022

In the heart of innate and adaptive immunity lies the proper spatiotemporal development of several immune cell lineages. Multiple studies have highlighted the necessity of epigenetic and transcriptional regulation in cell lineage specification. This mode of regulation is mediated by transcription factors and chromatin remodelers, controlling developmentally essential gene sets. The core of transcription and epigenetic regulation is formulated by different epigenetic modifications determining gene expression. Apart from “classic” epigenetic modifications, 3D chromatin architecture is also purported to exert fundamental roles in gene regulation. Chromatin conformation both facilitates cell-specific factor binding at specified regions and is in turn modified as such, acting synergistically. The interplay between global and tissue-specific protein factors dictates the epigenetic landscape of T and innate lymphoid cell (ILC) lineages. The expression of global genome organizers such as CTCF, YY1, and the cohesin complexes, closely cooperate with tissue-specific factors to exert cell type-specific gene regulation. Special AT-rich binding protein 1 (SATB1) is an important tissue-specific genome organizer and regulator controlling both long- and short-range chromatin interactions. Recent indications point to SATB1’s cooperation with the aforementioned factors, linking global to tissue-specific gene regulation. Changes in 3D genome organization are of vital importance for proper cell development and function, while disruption of this mechanism can lead to severe immuno-developmental defects. Newly emerging data have inextricably linked chromatin architecture deregulation to tissue-specific pathophysiological phenotypes. The combination of these findings may shed light on the mechanisms behind pathological conditions.
Assessing the Effectiveness of Conservation Stocking for the Endangered White’s Seahorse Hippocampus whitei
Authors: Harasti David , Brennan Mitchell , Booth David J.
Published: June 2022

The White’s seahorse Hippocampus whitei was listed as an Endangered species in 2020 on Australian state and federal legislation, as a result of population declines across its range attributed to habitat loss over the past decade. A captive-bred reintroduction program has been implemented as a possible management tool for species recovery, however, the viability of such a reintroduction program for seahorses has not been assessed to date. This study implemented a pilot captive-breeding program using adult H. whitei from Sydney Harbour, Australia, as brood stock. A total of 90 captive-bred seahorses were released into the wild on two different artificial habitat types (“seahorse hotels” and protective swimming net). Following release, a monthly post-release monitoring program was implemented for 12 months that involved underwater visual census surveys of the tagged seahorses. Sightings of captive-bred seahorse numbers were found to gradually decline over the 12-month period, with fewer seahorses found on the swimming net compared to the seahorse hotels and higher resighting probability of captive-bred animals on the seahorse hotels. After 12 months, 20% of the captive-bred seahorses were detected on the seahorse hotels, whilst two individuals were still observed 18 months after release on the hotels. Only 2% of captive-bred seahorses were observed on the swimming net after 12 months, with two individuals still detected on the net after two years. Nine of the captive-bred seahorses were found to reproduce in the wild, with two individuals observed mating with the wild population. This pilot study indicates that captive-bred seahorses can survive for up to two years in the wild, as well as contribute to local population recovery through reproductive success. However, while conservation stocking shows promise as a potential management tool to assist with threatened seahorse species recovery, there are several factors such as existing threats to the species that need to be addressed before such a program is implemented.
Hydrodynamic Interactions Between Macroalgae and Their Epibionts
Authors: Koehl M.A.R. , Daniel Thomas L.
Published: June 2022

Macroalgae provide surfaces where other organisms live. Unlike organisms on rigid substrata, epibionts on host macroalgae sit on flexible surfaces that bend, stretch, and move in turbulent water currents and waves. We used blade-like red algae,Mazzaella splendens, and encrusting bryozoans,Membranipora membranacea, to investigate the biomechanical and hydrodynamic effects of encrusting epibionts on macroalgae, and of flexible hosts on epibiotic bryozoans. Passive flapping by algae in wave-driven ambient flow enhanced renewal of water near hosts and epibionts. Wave exposure and the presence of a surrounding canopy of flexible algae altered the locations along algal blades where bryozoans encountered the highest time-averaged boundary shear velocities. Hydrodynamic forces on flexible algae moving back-and-forth with the water were lower in waves than in unidirectional flow. Bryozoan epibionts increased hydrodynamic forces on host algae by affecting their reconfiguration in moving water. Encrusting bryozoans increased the flexural stiffness of algal blades, but the elastic modulus, extensibility, and strength of blade tissue was unaffected by bryozoan epibionts. Algal blades were more extensible and stronger than bryozoans, so bryozoans fractured or popped off stretched algae. Algae in rapid-flow habitats had few epibionts, and encrusted algae transplanted from a protected to a wave-exposed habitat lost their epibionts.
Spatial and Seasonal Variations in the Stable Isotope Values and Trophic Positions of Dominant Zooplankton Groups in Jiaozhou Bay, China
Authors: Ke Zhixin , Li Ruofei , Chen Danting , Zhao Chunyu , Tan Yehui
Published: June 2022

The spatial and seasonal distributions of stable carbon and nitrogen isotopes (δ13C and δ15N) in dominant zooplankton groups were investigated in Jiaozhou Bay. Zooplankton δ13C values ranged from −22.89‰ to −15.86‰, and δ15N values ranged from 3.18‰ to 13.57‰, respectively. The δ13C and δ15N values generally followed the order of small zooplankton < large calanoids < small Sagitta < large Sagitta. Spatial distribution patterns of zooplankton δ13C and δ15N values varied in different seasons. Our results suggested that the spatial variation of δ13C was mainly controlled by terrigenous organic matter (OM) input and phytoplankton biomass, but water temperature may have played a key role in the seasonal variation of δ13C. In spring, the high phytoplankton biomass might increase the δ13C value of small zooplankton in the inner bay. During other seasons, the δ13C values of zooplankton generally increased from the inner bay to the outer bay, which might be associated with the influence of 13C-depleted terrigenous OM carried by the river discharge. Small zooplankton stable isotope values were significantly correlated with that of particulate organic matter (POM). The influence of anthropogenic nutrient input on isotopic baseline can be cascaded to the zooplankton, and the effect might be weak at higher trophic levels. The Bayesian standard ellipse areas of dominant zooplankton groups were generally smallest in the winter, suggesting a narrow niche width during that time. The niche partition between small zooplankton, large calanoids, and Sagitta was most distinct in winter, and followed by summer. The relative trophic level of Sagitta ranged from 2.23 to 4.01, which generally declined from the inner bay to the outer bay during the spring, autumn, and winter seasons. High anthropogenic nutrient loading might reduce the difference in trophic niches among zooplankton groups. This study provided detailed information on the distribution of zooplankton δ13C and δ15N in a coastal bay, which will be useful for understanding the anthropogenic influence on the ecosystem structure and function.
Predicting the Effects of Climate Change on the Suitable Habitat of Japanese Spanish Mackerel (Scomberomorus niphonius) Based on the Species Distribution Model
Authors: Yang Tianyan , Liu Xingyu , Han Zhiqiang
Published: June 2022

Japanese Spanish mackerel (Scomberomorus niphonius) is an important commercial fish species with a seasonal migratory habit in China. At present, no study has reported the seasonal variation of its potential suitable habitat. Therefore, we used biomod2 ensemble model to predict the potential suitable habitat of S. niphonius in different seasons through its current distribution sites and future environmental variables. Our results showed that the ensemble model had a high accuracy, with true skill statistics and receiver operator characteristic values of 0.91 and 0.98, respectively. Under the Representative Concentration Pathway (RCP) 4.5 scenario, the reduction rate of suitable habitats in summer of 2050 will be 33.2%, and those for the rest of the seasons will reach 3.5%. The reduction rate in summer of 2100 will be 43.5%, and those for the remaining seasons will reach 6.5%. In addition, this reduction rate will become more serious with the increase in emission scenario and time, especially in the RCP8.5 in which the summer reduction rate is as high as 88.9%. Habitat changes in summer show a strong temperature sensitivity. The distribution centroid of S. niphonius will shift to higher latitudes under RCP 4.5 and RCP 8.5. Depth and temperature are the two most important factors that affect the distribution of this species. The results can provide basic data for understanding the adaptability of S. niphonius to climate change and establishing an elastic ecosystem.
Heterotopic Pregnancy Secondary to in vitro Fertilization-Embryo Transfer: Risk Factors and Pregnancy Outcomes
Authors: Zhu Shaomi , Fan Yiyue , Lan Ling , Deng Tianqing , Zhang Qinxiu
Published: June 2022

Background

With the widespread application of assisted reproduction technology (ART) and increased pelvic inflammatory disease, the incidence of heterotopic pregnancy (HP) has risen. However, the risk factors and treatment of HP remain indefinite.

Objectives

To explore risk factors affecting the incidence of HP secondary to in vitro fertilization-embryo transfer (IVF-ET) and pregnancy outcomes after surgical treatment of HP.

Methods

29 patients with HP and 116 with an intrauterine-only pregnancy (IUP) after IVF-ET during the same period were included retrospectively from January 2015 to September 2020.

Results

Patients with HP had a higher proportion of previous ectopic pregnancies, multiple abortion history (≧2 times) and tubal indication for IVF than IUP. Besides, they had a greater possibility to end in spontaneous abortion (31.03 vs.13.79%, P = 0.028) and preterm delivery (25.00 vs. 7.00%, P = 0.035), less possibility to result in a live birth (58.62 vs. 78.45%, P = 0.028). History of multiple abortions (≥2 times) [odds ratio (OR) 3.031, 95% confidence intervals (CI) 1.087–8.453; P = 0.034], tubal infertility (OR 3.844, 95% CI 1.268–11.656; P = 0.017), previous ectopic pregnancies (OR 2.303, 95% CI 0.625–8.490; P = 0.021) and number of embryo transfer (OR 0.300, 95% CI 0.092–0.983; P = 0.037) resulted in an elevated proportion of HP in IVF treatment. Shorter operative duration, smaller size of the ectopic mass and location in the ampulla of the fallopian tube were associated with higher chance of survival in the coexistent intrauterine pregnancy after surgical treatment.

Conclusions

Previous history of ectopic pregnancy, multiple abortions, tubal infertility and multiple-embryo transfer may be considered as meaningful risk factors of subsequent HP following IVF-ET. In patients with HP treated by surgery, shorter operative duration, smaller size of the ectopic mass and location in the ampulla of the fallopian tube means better reproductive prognosis.
The Evaluation of Physiological Index Changes and Safety Work of Female Medical Staff With Different Medical Protection Standards in the Ward of COVID-19
Authors: Zhao Min , Zhao Jianhui , Yan Junbing , Gao Xiaoye
Published: June 2022

Background

Effective personal protective equipment (PPE) contribute to the prevention of COVID-19 infection. However, it is necessary to evaluate the potential risk of different medical protections in the isolation ward of COVID-19.

Objectives

We aimed to explore the dynamics in physiological indexes of medical staff under primary and secondary PPE in the isolation ward of COVID-19 and provide the scientific basis for determining the safe work strategy.

Materials and Methods

In this study, 30 female nurses were selected to simulate medical work under the primary or secondary PPE, respectively. The oral temperature, axillary temperature, heart rate, respiratory rate, blood oxygen saturation, and blood pressure were measured and recorded every 20 min. The subjective adverse symptoms were recorded every 30 min. The blood glucose and weight of the individuals were measured and recorded before and after the trial.

Results

The results indicated that the median trial persistence time in the participants with moderate-intensity work wearing the secondary PPE (70.0 min) was much lower than that with moderate-intensity work wearing the primary PPE (180 min) and with light-intensity work wearing the primary PPE (110 min; p < 0.05). Importantly, the heart rate, oral/axillary temperature, and respiratory rate of physiological indexes of the participants under moderate-intensity work wearing the secondary PPE increased significantly faster than the primary PPE (p < 0.001), while blood oxygen saturation decreased significantly faster than the primary PPE (p < 0.001). In addition, the proportions of subjective adverse symptoms (such as dry mouth, dizziness, palpitations, and anhelation) were much higher than primary PPE (p < 0.001). The average sweat volume and blood glucose consumption of participants under moderate-intensity work wearing primary PPE were higher than secondary PPE (p < 0.001).

Conclusion

The combination of an exacerbated workload and secondary PPE worn by COVID-19 healthcare workers increases the change in physiological indicators, and in some cases the adverse symptoms, which can affect and even suspend their medical work. For any medical institution, there is room for improvement in terms of bioethics of a “Job Well Done” to reduce the risks of medical activities under secondary PPE.
Healthy Organs Uptake on Baseline 18F-FDG PET/CT as an Alternative to Total Metabolic Tumor Volume to Predict Event-Free Survival in Classical Hodgkin's Lymphoma
Authors: Morland David , Triumbari Elizabeth Katherine Anna , Maiolo Elena , Cuccaro Annarosa , Treglia Giorgio , Hohaus Stefan , Annunziata Salvatore
Published: June 2022

Purpose

Healthy organs uptake, including cerebellar and liver SUVs have been reported to be inversely correlated to total metabolic tumor volume (TMTV), a controversial predictor of event-free survival (EFS) in classical Hodgkin's Lymphoma (cHL). The objective of this study was to estimate TMTV by using healthy organs SUV measurements and assess the performance of this new index (UF, Uptake Formula) to predict EFS in cHL.

Methods

Patients with cHL were retrospectively included. SUV values and TMTV derived from baseline 18F-FDG PET/CT were harmonized using ComBat algorithm across PET/CT systems. UF was estimated using ANOVA analysis. Optimal thresholds of TMTV and UF were calculated and tested using Cox models.

Results

163 patients were included. Optimal UF model of TMTV included age, lymphoma maximum SUVmax, hepatic SUVmean and cerebellar SUVmax (R2 14.0% - p < 0.001). UF > 236.8 was a significant predictor of EFS (HR: 2.458 [1.201–5.030], p = 0.01) and was not significantly different from TMTV > 271.0 (HR: 2.761 [1.183–5.140], p = 0.001). UF > 236.8 remained significant in a bivariate model including IPS score (p = 0.02) and determined two populations with different EFS (63.7 vs. 84.9%, p = 0.01).

Conclusion

The Uptake Formula, a new index including healthy organ SUV values, shows similar performance to TMTV in predicting EFS in Hodgkin's Lymphoma. Validation cohorts will be needed to confirm this new prognostic parameter.
Metabolic Fingerprint Analysis of Cytochrome b5-producing E. coli N4830-1 Using FT-IR Spectroscopy
Authors: Tengsuttiwat Thanyaporn , Kaderbhai Naheed Nazly , Gallagher Joe , Goodacre Royston , Muhamadali Howbeer
Published: June 2022

Optimization of recombinant protein expression in bacteria is an important task in order to increase protein yield while maintaining the structural fidelity of the product. In this study, we employ Fourier transform infrared (FT-IR) spectroscopy as a high throughput metabolic fingerprinting approach to optimize and monitor cytochrome b5 (CYT b5) production in Escherichia coli N4830-1, as the heterologous host. Cyt b5 was introduced as a plasmid with between 0 and 6 copies under a strong promoter. The FT-IR spectroscopy results combined with multivariate chemometric analysis illustrated discriminations among culture conditions as well as revealing features that correlated to the different cytb5 gene copy numbers. The second derivative of the FT-IR spectral data allowed for the quantitative detection of Cyt b5 directly inside the intact cells without the need for extraction, and highlighted changes in protein secondary structure that was directly correlated to the cytb5 gene copy number and protein content, and was in complete agreement with quantitative findings of standard traditional techniques such as SDS–PAGE and western blot analysis.