bluuebunny/crossref_metadata_2025 huggingface dataset:
A web-based decision support framework for optimizing road network accessibility and emergency facility allocation during flooding
Authors: Alabbad Yazeed , Mount Jerry , Campbell Ann M. , Demir Ibrahim
Published: March 2024

Transportation systems can be significantly affected by flooding, leading to physical damage and hindering accessibility. Despite flooding being a frequent occurrence, there are limited accessible online tools available for supporting routing and emergency planning decisions during flooding. Existing tools are generally based on complicated models and are not easily accessible to non-expert users, highlighting the need for efficient communication and decision-making tools for analyzing flood impacts on transportation networks for various stakeholders, including the public, to minimize the adverse impacts on those groups. This paper presents a web application that uses graph network methods and the latest web technologies and standards to assist in describing flood events in terms of operational constraints and provide analytical methods to support mobility and mitigation decisions during these events. The framework is designed to be user-friendly, enabling non-expert users to access information about road status, shortest paths to critical amenities, location-allocation, and service coverage. The study area includes the following two communities in the State of Iowa, Cedar Rapids and Charles City, which were used to test the application's functionality and explore the outcomes. Our research demonstrates that flooding can significantly affect bridge operation, routing from locations to critical amenities, arbitrary point-to-point routing, planning for emergency facility placement, and service area accessibility. The introduced framework can solve complex flood-related analytical decision tasks and provide an understandable representation of transportation vulnerability, enhancing mitigation strategies. Therefore, this web application provides a valuable tool for stakeholders to make informed decisions on transportation networks during flood events.
India’s policy response to the COVID-19 pandemic: Lessons for a post-COVID society
Authors: Kandpal Prakash Chand
Published: March 2024

The COVID-19 pandemic has left an indelible mark on societies worldwide, challenging governments to respond swiftly and effectively to mitigate its impact. India, with its vast population and complex healthcare landscape, faced unique challenges in formulating and implementing a pandemic response strategy. The article examines India's policy response to the COVID-19 pandemic and explores the valuable lessons it offers for shaping a more resilient and prepared society in a post-COVID world. It provides a comprehensive analysis of India's multifaceted approach to managing the pandemic, highlighting key elements such as lockdowns, testing and contact tracing, healthcare infrastructure, vaccination drives, and economic relief measures. By delving into both the successes and shortcomings of these policies, it seeks to extract valuable insights for policymakers and public health officials globally. As the world transitions into a post-COVID era, the lessons learned from India's experience offer a roadmap for building stronger healthcare systems, improving disaster preparedness, and enhancing social safety nets. The article underscores the importance of proactive governance, community engagement, data-driven decision-making, and international collaboration in the face of global health crises. The paper demonstrates that India's journey through the pandemic provides a wealth of knowledge that can inform policy development, foster greater resilience, and help societies better navigate the uncertainties of a post-COVID world. By reflecting on the successes and challenges of India's response, this article offers actionable insights for shaping a more equitable, sustainable, and prepared society in the wake of the COVID-19 pandemic.
Authorship Analysis in Chinese Social Media Texts
Authors: Zhang Shaomin
Published: March 2024

This Element explores the sentiment and keyword features in both authorship profiling and authorship attribution in social media texts in the Chinese cultural context. The key findings can be summarised as follows: firstly, sentiment scores and keyword features are distinctive in delineating authors' gender and age. Specifically, female and younger authors tend to be less optimistic and use more personal pronouns and graduations than male and older authors, respectively. Secondly, these distinctive profiling features are also distinctive and significant in authorship attribution. Thirdly, our mindset, shaped by our inherent hormonal influences and external social experiences, plays a critical role in authorship. Theoretically, the findings expand authorship features into underexplored domains and substantiate the theory of mindset. Practically, the findings offer some broad quantitative benchmarks for authorship profiling cases in the Chinese cultural context, and perhaps other contexts where authorship profiling analyses have been used. This title is also available as Open Access on Cambridge Core.
Contrast-Induced Encephalopathy and the Blood-Brain Barrier
Authors: Maclean Mark A. , Rogers Patrick S. , Muradov Jamil H. , Pickett Gwynedd E. , Friedman Alon , Weeks Adrienne , Greene Ryan , Volders David
Published: March 2024

Background:

Contrast-induced encephalopathy (CIE) is an adverse event associated with diagnostic and therapeutic endovascular procedures. Decades of animal and human research support a mechanistic role for pathological blood-brain barrier dysfunction (BBBd). Here, we describe an institutional case series and review the literature supporting a mechanistic role for BBBd in CIE.

Methods:

A literature review was conducted by searching MEDLINE, Web of Science, Embase, CINAHL and Cochrane databases from inception to January 31, 2022. We searched our institutional neurovascular database for cases of CIE following endovascular treatment of cerebrovascular disease during a 6-month period. Informed consent was obtained in all cases.

Results:

Review of the literature revealed risk factors for BBBd and CIE, including microvascular disease, pathological neuroinflammation, severe procedural hypertension, iodinated contrast load and altered cerebral blood flow dynamics. In our institutional series, 6 of 52 (11.5%) of patients undergoing therapeutic neuroendovascular procedures developed CIE during the study period. Four patients were treated for ischemic stroke and two patients for recurrent cerebral aneurysms. Mechanical stenting or thrombectomy were utilized in all cases.

Conclusion:

In this institutional case series and literature review of animal and human data, we identified numerous shared risk factors for CIE and BBBd, including microvascular disease, increased procedure length, large contrast volumes, severe intraoperative hypertension and use of mechanical devices that may induce iatrogenic endothelial injury.
Strategies to improve the implementation and effectiveness of community-based psychosocial support interventions for displaced, migrant and host community women in Latin America
Authors: Greene M. Claire , Wimer Gabrielle , Larrea Maria , Mejia Jimenez Ingrid , Armijos Andrea , Angulo Alejandra , Guevara Maria Esther , Vega Carolina , Heard Emily W. , Demis Lina , Benavides Lucia , Corrales Christine , de la Cruz Ale , Lopez Maria Jose , Moyano Arianna , Murcia Andrea , Noboa Maria Jose , Rodriguez Abhimeleck , Solis Jennifer , Vergara Daniela , Andersen Lena S. , Cristobal Maria , Wainberg Milton , Bonz Annie G. , Tol Wietse
Published: March 2024

As evidence supporting the effectiveness of mental health and psychosocial interventions grows, more research is needed to understand optimal strategies for improving their implementation in diverse contexts. We conducted a qualitative process evaluation of a multicomponent psychosocial intervention intended to promote well-being among refugee, migrant and host community women in three diverse contexts in Ecuador and Panamá. The objective of this study is to describe the relationships among implementation determinants, strategies and outcomes of this community-based psychosocial intervention. The five implementation strategies used in this study included stakeholder engagement, promoting intervention adaptability, group and community-based delivery format, task sharing and providing incentives. We identified 10 adaptations to the intervention and its implementation, most of which were made during pre-implementation. Participants (n = 77) and facilitators (n = 30) who completed qualitative interviews reported that these strategies largely improved the implementation of the intervention across key outcomes and aligned with the study’s intervention and implementation theory of change models. Participants and facilitators also proposed additional strategies for improving reach, implementation and maintenance of this community-based psychosocial intervention.
Case C-632/20 P, Spain v. Eur. Comm'n (C.J.E.U.)
Authors: Kassoti Eva
Published: March 2024

On January 17, 2023, the Grand Chamber of the Court of Justice of the European Union (CJEU) delivered its judgments in case C-632/20, P Spain v. Commission (Kosovo), ruling that notwithstanding the European Union's non-recognition of Kosovo as a state, Kosovo may participate in an EU agency, namely the Body of European Regulators for Electronic Communications (BEREC). The judgment is significant for several reasons: (1) for the European Union's growing engagement with non-recognized territorial entities since it clarifies the meaning of the concept of “third country” and confirms that such entities may participate in EU agencies; (2) for the European Union's engagement with Kosovo—particularly in the light of Kosovo's 2022 bid for EU membership; and (3) more broadly, in the context of the burgeoning debate regarding the CJEU's approach to international law.
A NEW PERSPECTIVE ON SEMI-RETRACTIONS AND THE RAMSEY PROPERTY
Authors: BARTOŠOVÁ DANA , SCOW LYNN
Published: March 2024

We investigate the notion of a semi-retraction between two first-order structures (in typically different signatures) that was introduced by the second author as a link between the Ramsey property and generalized indiscernible sequences. We look at semi-retractions through a new lens establishing transfers of the Ramsey property and finite Ramsey degrees under quite general conditions that are optimal as demonstrated by counterexamples. Finally, we compare semi-retractions to the category theoretic notion of a pre-adjunction.
Developing a human rights compatible governance framework for quantum computing
Authors: van Daalen Ot
Published: March 2024

Quantum computers hold significant promise for peaceful applications, but one of the more immediate potential applications is breaking of public key encryption technologies. This poses significant risks to the information security of global digital infrastructure in a broader sense. At the same time, the development of quantum computing is a quintessentially scientific undertaking. There is a tension in the scientific freedom required to develop these technologies, and the measures to mitigate the risks associated with quantum computers. Policy for resolving this tension must be in line with the human right to science, read together with the right to privacy and the right to freedom of expression. In this article, I apply these rights to the development of quantum computing to provide guidance for government policy on quantum computing. I conclude that states must create the conditions for scientific research to flourish, even if this research may carry significant societal risks. This applies also to research and development of quantum technologies. In the context of quantum computing, this primarily means investing in the development and uptake of alternative encryption technologies which are resistant to attacks by quantum computers. It also means regulating the use of these technologies for applications which are undesirable.
“Yugoslavia has Nothing. Yugoslavia has No Bread. But Hungary Gives Us Bread”: Access to Food and (Dis)loyalty in a “Redeemed” Yugoslav Borderland
Authors: Kosi Jernej
Published: March 2024

This article illustrates the socioeconomic background of rural political discontent in the post-imperial Yugoslav border region Prekmurje. The author argues that during the post-Habsburg political transition and ensuing social transformation, the fundamental lack of loyalty to the Yugoslav state among an important segment of the rural population of Prekmurje was rooted in insufficient access to food. Documents of court proceedings, official state reports, and findings of individuals with deep understandings of the situation on the ground reveal that this rural political mobilization was not so much a reflection of Hungarian propaganda or a “lack of appropriate national identification” among the local population—although, of course, these two factors cannot be ignored in a contested and linguistically and ethnically diverse region—but rather an outcome of the impoverishment of large sections of the peasant population.
Waylosing and wayfinding in the outdoors: a typology of wayfinding approaches to problem-solving when temporarily lost
Authors: Wattne Ole Edward , Volden Frode
Published: March 2024

This paper presents the findings from a survey conducted in Norway to study the process of recovering from temporary disorientation in outdoor environments. The survey, with 693 respondents, investigated how individuals navigate and regain their bearings after getting disoriented for a short period of time. By collecting data on duration of disorientation and descriptions of participants' recovery experiences, we conducted both qualitative and quantitative analyses to establish a typology of spatial problem-solving [Downs, R. M. and Stea, D. (1977). Maps in Minds: Reflections on Cognitive Mapping. New York: Harper & Row, p. 55] approaches employed in wayfinding after becoming temporarily lost in outdoor settings. The research systematically explores the use of materials and approaches described by respondents when re-establishing their bearings. The existing research literature lacks comprehensive reporting on people's strategies for solving the problem of being disoriented in the outdoors, which motivated us to conduct this study. The resulting typology gives an overview of approaches employed to solve the problem of being lost in the outdoors and contribute additional details and insights to the understanding of individuals' wayfinding behaviours and reorientation processes.
Emergence and evolution of developmental resilience science over half a century
Authors: Masten Ann S.
Published: March 2024

This reflection on the history and future of developmental resilience science (DRS) highlights its co-emergence with developmental psychopathology (DP), as well as the roles of this journal and its founding editor, Dante Cicchetti, in the evolution of these intertwined domains of scholarship. A remarkable constellation of scholars at the University of Minnesota shaped the course of both conceptual frameworks and their dissemination. I describe fundamental assumptions common to DP and DRS frameworks that reflect their common roots and the pervasive influence of systems theory on developmental science. I describe four waves of DRS and key principles of DRS at the present time. In conclusion, I consider the possibility that a fifth wave of DRS is emerging with a focus on understanding patterns of multisystem, multilevel processes of resilience and their implications for interventions in the context of interacting, interdependent, and complex adaptive systems. I close this commentary with questions for future research and a hopeful outlook on the future of human resilience.
Facilitating clinical use of the Amsterdam Instrumental Activities of Daily Living Questionnaire: Normative data and a diagnostic cutoff value
Authors: Postema Merel C. , Dubbelman Mark A. , Claesen Jürgen , Ritchie Craig , Verrijp Merike , Visser Leonie , Visser Pieter-Jelle , Zwan Marissa D. , van der Flier Wiesje M. , Sikkes Sietske A.M.
Published: March 2024

Objective:

The Amsterdam Instrumental Activities of Daily Living Questionnaire (A-IADL-Q) is well validated and commonly used to assess difficulties in everyday functioning regarding dementia. To facilitate interpretation and clinical implementation across different European countries, we aim to provide normative data and a diagnostic cutoff for dementia.

Methods:

Cross-sectional data from Dutch Brain Research Registry (N = 1,064; mean (M) age = 62 ± 11 year; 69.5% female), European Medial Information Framework-Alzheimer’s Disease 90 + (N = 63; Mage = 92 ± 2 year; 52.4% female), and European Prevention of Alzheimer’s Dementia Longitudinal Cohort Study (N = 247; Mage = 63 ± 7 year; 72.1% female) were used. The generalized additive models for location, scale, and shape framework were used to obtain normative values (Z-scores). The beta distribution was applied, and combinations of age, sex, and educational attainment were modeled. The optimal cutoff for dementia was calculated using area under receiver operating curves (AUC-ROC) and Youden Index, using data from Amsterdam Dementia Cohort (N = 2,511, Mage = 64 ± 8 year, 44.4% female).

Results:

The best normative model accounted for a cubic-like decrease of IADL performance with age that was more pronounced in low compared to medium/high educational attainment. The cutoff for dementia was 1.85 standard deviation below the population mean (AUC = 0.97; 95% CI [0.97–0.98]).

Conclusion:

We provide regression-based norms for A-IADL-Q and a diagnostic cutoff for dementia, which help improve clinical assessment of IADL performance across European countries.
From Conceptual Gaps to Policy Dialogue: Conceptual Approaches to Disability and Old Age in Ageing Research and Disability Studies
Authors: Era Salla , Katsui Hisayo , Kröger Teppo
Published: March 2024

In this study, we investigated the conceptual approaches to disability and ageing in two leading social scientific journals (Ageing & Society[AS] andDisability & Society[DS]) of the research fields that form the bases of policies on disability and ageing. This study aimed to identify the journals’ trajectories of conceptual development and their differences, and through that, find possible pathways for further interaction between the yet largely separate policy frameworks for disability and ageing. Our analysis showed considerable differences between the conceptual approaches of the two journals, with the dominant approach in DS being sociomaterial and individual-functional in AS. We conclude this paper by identifying the conceptual gaps in the respective journals, suggesting a further collaboration between the approaches in research as well as policies. These gaps could be potentially narrowed, leading to a constructive dialogue on older disabled people.
Calibration of Swarm Ion Density, Drift, and Effective Mass Measurements
Authors: Burchill Johnathan K. , Lomidze Levan
Published: March 2024

We calibrate the Swarm Langmuir Probe Ion Drift, Density and Effective Mass (SLIDEM) products using the Constellation Observing System for Meteorology, Ionosphere and Climate (COSMIC‐1) electron density measurements. SLIDEM combines electric current measurements from the Electric Field Instrument faceplate (situated on the ram‐facing side of the satellite) with ion admittance measurements from a spherical Langmuir probe to estimate ionospheric ion density, bulk ion drift parallel to the satellite trajectory in the polar regions, and effective ion mass at low and middle latitudes. SLIDEM processing includes an eight‐parameter empirical geometry model that expresses the corrections to the effective faceplate area and spherical probe radius arising from satellite‐plasma interactions. Validation of SLIDEM density by conjunctions with COSMIC density altitude profiles reveals systematic errors, with SLIDEM underestimating corresponding COSMIC data by around 10%. Using the same conjunctions to calibrate the SLIDEM density processor, a simple one‐parameter geometry model is obtained, which significantly reduces bias in ion density. This leads to reduced bias in the ion ram speed and in the effective mass, both of which are more consistent with empirical ionospheric models. We examined three models characterizing the variation of probe effective electrode current collection areas. Faceplate geometries are statistically indistinguishable from the physical faceplate cross section. Spherical Langmuir probe radius is effectively reduced by about 10% due to satellite‐plasma interactions. We recommend updating the SLIDEM processor to use a smaller value for the spherical probe's radius.
Making Ecosystem Modeling Operational–A Novel Distributed Execution Framework to Systematically Explore Ecological Responses to Divergent Climate Trajectories
Authors: Steenbeek Jeroen , Ortega Pablo , Bernardello Raffaele , Christensen Villy , Coll Marta , Exarchou Eleftheria , Fuster‐Alonso Alba , Heneghan Ryan , Julià Melis Laura , Pennino Maria Grazia , Rivas David , Keenlyside Noel
Published: March 2024

Marine Ecosystem Models (MEMs) are increasingly driven by Earth System Models (ESMs) to better understand marine ecosystem dynamics, and to analyze the effects of alternative management efforts for marine ecosystems under potential scenarios of climate change. However, policy and commercial activities typically occur on seasonal‐to‐decadal time scales, a time span widely used in the global climate modeling community but where the skill level assessments of MEMs are in their infancy. This is mostly due to technical hurdles that prevent the global MEM community from performing large ensemble simulations with which to undergo systematic skill assessments. Here, we developed a novel distributed execution framework constructed of low‐tech and freely available technologies to enable the systematic execution and analysis of linked ESM/MEM prediction ensembles. We apply this framework on the seasonal‐to‐decadal time scale, and assess how retrospective forecast uncertainty in an ensemble of initialized decadal ESM predictions affects a mechanistic and spatiotemporal explicit global trophodynamic MEM. Our results indicate that ESM internal variability has a relatively low impact on the MEM variability in comparison to the broad assumptions related to reconstructed fisheries. We also observe that the results are also sensitive to the ESM specificities. Our case study warrants further systematic explorations to disentangle the impacts of climate change, fisheries scenarios, MEM internal ecological hypotheses, and ESM variability. Most importantly, our case study demonstrates that a simple and free distributed execution framework has the potential to empower any modeling group with the fundamental capabilities to operationalize marine ecosystem modeling.
Myelodysplastic neoplasms dissected into indolent, leukaemic and unfavourable subtypes by computational clustering of haematopoietic stem and progenitor cells
Authors: van Spronsen Margot F. , Van Gassen Sofie , Duetz Carolien , Westers Theresia M. , Saeys Yvan , van de Loosdrecht Arjan A.
Published: March 2024

Myelodysplastic neoplasms (MDS) encompass haematological malignancies, which are characterised by dysplasia, ineffective haematopoiesis and the risk of progression towards acute myeloid leukaemia (AML). Myelodysplastic neoplasms are notorious for their heterogeneity: clinical outcomes range from a near-normal life expectancy to leukaemic transformation or premature death due to cytopenia. The Molecular International Prognostic Scoring System made progress in the dissection of MDS by clinical outcomes. To contribute to the risk stratification of MDS by immunophenotypic profiles, this study performed computational clustering of flow cytometry data of CD34+ cells in 67 MDS, 67 AML patients and 49 controls. Our data revealed heterogeneity also within the MDS-derived CD34+ compartment. In MDS, maintenance of lymphoid progenitors and megakaryocytic-erythroid progenitors predicted favourable outcomes, whereas expansion of granulocyte-monocyte progenitors increased the risk of leukaemic transformation. The proliferation of haematopoietic stem cells and common myeloid progenitors with downregulated CD44 expression, suggestive of impaired haematopoietic differentiation, characterised a distinct MDS subtype with a poor overall survival. This exploratory study demonstrates the prognostic value of known and previously unexplored CD34+ populations and suggests the feasibility of dissecting MDS into a more indolent, a leukaemic and another unfavourable subtype.
Transient stimulated Raman scattering spectroscopy and imaging
Authors: Yu Qiaozhi , Yao Zhengjian , Zhou Jiaqi , Yu Wenhao , Zhuang Chenjie , Qi Yafeng , Xiong Hanqing
Published: March 2024

Stimulated Raman scattering (SRS) has been developed as an essential quantitative contrast for chemical imaging in recent years. However, while spectral lines near the natural linewidth limit can be routinely achieved by state-of-the-art spontaneous Raman microscopes, spectral broadening is inevitable for current mainstream SRS imaging methods. This is because those SRS signals are all measured in the frequency domain. There is a compromise between sensitivity and spectral resolution: as the nonlinear process benefits from pulsed excitations, the fundamental time-energy uncertainty limits the spectral resolution. Besides, the spectral range and acquisition speed are mutually restricted. Here we report transient stimulated Raman scattering (T-SRS), an alternative time-domain strategy that bypasses all these fundamental conjugations. T-SRS is achieved by quantum coherence manipulation: we encode the vibrational oscillations in the stimulated Raman loss (SRL) signal by femtosecond pulse-pair sequence excited vibrational wave packet interference. The Raman spectrum was then achieved by Fourier transform of the time-domain SRL signal. Since all Raman modes are impulsively and simultaneously excited, T-SRS features the natural-linewidth-limit spectral line shapes, laser-bandwidth-determined spectral range, and improved sensitivity. With ~150-fs laser pulses, we boost the sensitivity of typical Raman modes to the sub-mM level. With all-plane-mirror high-speed time-delay scanning, we further demonstrated hyperspectral SRS imaging of live-cell metabolism and high-density multiplexed imaging with the natural-linewidth-limit spectral resolution. T-SRS shall find valuable applications for advanced Raman imaging.
Cost-utility analysis and impact on the environment of videoconference in pressure injury. A randomized controlled trial in individuals with spinal cord injury
Authors: Irgens Ingebjørg , Kleven Linn , Midelfart-Hoff Jana , Jelnes Rolf , Alexander Marcalee , Stanghelle Johan K. , Rekand Tiina
Published: March 2024

Study design

A prospective randomized controlled trial (RCT) in persons with spinal cord injury (SCI) and ongoing pressure injury (PI).

Objectives

The main aim was to perform a cost-utility analysis (CUA) alongside the RCT comparing regular care to regular care with additional videoconference consultations. Secondary aims were to assess costs and greenhouse gas emission related to transportation in the two study groups.

Setting

Two spinal cord units in Norway.

Methods

Participants were allocated to a regular care group (RCG) and a regular care group with additional videoconference (VCG), in a 1-year follow-up between 2016 and 2018. Costs were prospectively collected, and health-related quality of life (HRQoL) data were collected at baseline and 12 months. The outcome was quality-adjusted life years (QALYs), derived from the EQ-5D-5L questionnaire. Results are reported as incremental cost-effectiveness ratio (ICER), expressed as the cost per additional QALY gained. Transportation related costs and environmental emissions were compared by t-tests.

Results

There were 56 participants included, 28 in each group. Of these 27 in the VCG and 26 in the RCG completed. Three participants died. The mean cost per patient was € 8819 in the VCG and € 3607 in the RCG, with 0.1 QALYs gained in the VCG. No significant differences were identified regarding HRQoL or secondary outcomes.

Conclusion

The VCG costs € 5212 more for an additional 0.1 QALYs, giving an ICER of € 52,120 per QALY. No significant differences were found regarding transportation-related costs, or emission of greenhouse gases.

Trial registration

www.ClinicalTrials.gov; NCT02800915, TeleSCIpi. CRISTIN.no. https://app.cristin.no/projects/show.jsf?id=545284. Sunnaas Rehabilitation hospital’s web page, available at https://www.sunnaas.no/fag-og-forskning/fagstoff/sar.
STING dependent BAX-IRF3 signaling results in apoptosis during late-stage Coxiella burnetii infection
Authors: Chauhan Manish , Osbron Chelsea A. , Koehler Heather S. , Goodman Alan G.
Published: March 2024

STING (STimulator of Interferon Genes) is a cytosolic sensor for cyclic dinucleotides (CDNs) and initiates an innate immune response upon binding to CDNs. Coxiella burnetii is a Gram-negative obligate intracellular bacterium and the causative agent of the zoonotic disease Q fever. The ability of C. burnetii to inhibit host cell death is a critical factor in disease development. Previous studies have shown that C. burnetii inhibits host cell apoptosis at early stages of infection. However, during the late-stages of infection, there is host cell lysis resulting in the release of bacteria to infect bystander cells. Thus, we investigated the role of STING during late-stages of C. burnetii infection and examined STING’s impact on host cell death. We show that the loss of STING results in higher bacterial loads and abrogates IFNβ and IL6 induction at 12 days post-infection. The absence of STING during C. burnetii infection significantly reduces apoptosis through decreased caspase-8 and -3 activation. During infection, STING activates IRF3 which interacts with BAX. BAX then translocates to the mitochondria, which is followed by mitochondrial membrane depolarization. This results in increased cytosolic mtDNA in a STING-dependent manner. The presence of increased cytosolic mtDNA results in greater cytosolic 2′-3′ cGAMP, creating a positive feedback loop and leading to further increases in STING activation and its downstream signaling. Taken together, we show that STING signaling is critical for BAX-IRF3-mediated mitochondria-induced apoptosis during late-stage C. burnetii infection.
Immune response induced by standard and fractional doses of 17DD yellow fever vaccine
Authors: Abdala-Torres Thais , Campi-Azevedo Ana Carolina , da Silva-Pereira Rosiane Aparecida , dos Santos Luara Isabela , Henriques Priscilla Miranda , Costa-Rocha Ismael Artur , Otta Dayane Andriotti , Peruhype-Magalhães Vanessa , Teixeira-Carvalho Andréa , Araújo Márcio Sobreira Silva , Fernandes Eder Gatti , Sato Helena Keico , Fantinato Francieli Fontana Sutile Tardetti , Domingues Carla Magda Allan Santos , Kallás Esper Georges , Tomiyama Helena Tomoko Iwashita , Lemos Jandira Aparecida Campos , Coelho-dos-Reis Jordana Grazziela , de Lima Sheila Maria Barbosa , Schwarcz Waleska Dias , de Souza Azevedo Adriana , Trindade Gisela Freitas , Ano Bom Ana Paula Dinis , da Silva Andrea Marques Vieira , Fernandes Camilla Bayma , Camacho Luiz Antônio Bastos , de Sousa Maia Maria de Lourdes ,   Collaborative Group for Studies of Yellow Fever Vaccine, Martins-Filho Olindo Assis , do Antonelli Lis Ribeiro do Valle
Published: March 2024

The re-emergence of yellow fever (YF) urged new mass vaccination campaigns and, in 2017, the World Health Organization approved the use of the fractional dose (FD) of the YF vaccine due to stock shortage. In an observational cross-sectional investigation, we have assessed viremia, antibodies, soluble mediators and effector and memory T and B-cells induced by primary vaccination of volunteers with FD and standard dose (SD). Similar viremia and levels of antibodies and soluble markers were induced early after immunization. However, a faster decrease in the latter was observed after SD. The FD led to a sustained expansion of helper T-cells and an increased expression of activation markers on T-cells early after vaccination. Although with different kinetics, expansion of plasma cells was induced upon SD and FD immunization. Integrative analysis reveals that FD induces a more complex network involving follicular helper T cells and B-cells than SD. Our findings substantiate that FD can replace SD inducing robust correlates of protective immune response against YF.
An optogenetic method for the controlled release of single molecules
Authors: Kashyap Purba , Bertelli Sara , Cao Fakun , Kostritskaia Yulia , Blank Fenja , Srikanth Niranjan A. , Schlack-Leigers Claire , Saleppico Roberto , Bierhuizen Dolf , Lu Xiaocen , Nickel Walter , Campbell Robert E. , Plested Andrew J. R. , Stauber Tobias , Taylor Marcus J. , Ewers Helge
Published: March 2024

We developed a system for optogenetic release of single molecules in cells. We confined soluble and transmembrane proteins to the Golgi apparatus via a photocleavable protein and released them by short pulses of light. Our method allows for a light dose-dependent delivery of functional proteins to the cytosol and plasma membrane in amounts compatible with single-molecule imaging, greatly simplifying access to single-molecule microscopy of any protein in live cells. We were able to reconstitute ion conductance by delivering BK and LRRC8/volume-regulated anion channels to the plasma membrane. Finally we were able to induce NF-kB signaling in T lymphoblasts stimulated by interleukin-1 by controlled release of a signaling protein that had been knocked out. We observed light-induced formation of functional inflammatory signaling complexes that triggered phosphorylation of the inhibitor of nuclear factor kappa-B kinase only in activated cells. We thus developed an optogenetic method for the reconstitution and investigation of cellular function at the single-molecule level.
A chromosome-level genome for the flower thrips Frankliniella intonsa
Authors: Song Wei , Wang Jia-Xu , Cao Li-Jun , Chen Jin-Cui , Bao Wen-Xue , Chen Min , Wei Shu-Jun
Published: March 2024

The flower thrips Frankliniella intonsa (Thysanoptera: Thripidae) is a common insect found in flowers of many plants. Sometimes, F. intonsa causes damage to crops through direct feeding and transmission of plant viruses. Here, we assembled a chromosomal level genome of F. intonsa using the Illumina, Oxford Nanopore (ONT), and Hi-C technologies. The assembled genome had a size of 209.09 Mb, with a contig N50 of 997 bp, scaffold N50 of 13.415 Mb, and BUSCO completeness of 92.5%. The assembled contigs were anchored on 15 chromosomes. A set of 14,109 protein-coding genes were annotated in the genome with a BUSCO completeness of 95.0%. The genome contained 491 non-coding RNA and 0.57% of interspersed repeats. This high-quality genome provides a valuable resource for understanding the ecology, genetics, and evolution of F. intonsa, as well as for controlling thrips pests.
Orbit constraint of a small bead on a rotating large circular hoop in a horizontal plane
Authors: Wang Yuan-Sheng
Published: March 2024

Orbit constraint problems can be encountered in mechanical equipment and amusement equipment. Mechanics exercises generally consider the ideal physical model, and the practical problems also consider the influence of friction, which makes the problem more complex and and practical. The problem of the force and oscillation of objects on orbit needs to be deeply discussed. In order to simulate the orbital motion of objects more realistically and help students expand their theoretical mechanics beyond class, we study the orbit constraint of a small bead on a rotating large circular hoop in a horizontal plane about an axis passing through a point on the circumference. The coupling equations followed by the bead on the hoop are derived using Newton’s second law in a planar polar coordinate system and solved by numerical methods. We found that under the action of friction, when the initial angular velocity of the bead is greater than the critical angular velocity, the bead will rotate on the ring, and the number of rotations is related to the initial angular velocity and influenced by the friction coefficient. At different initial angular velocities, the number of oscillations of the bead on the hoop is basically the same and ultimately stops near the fixed point.
A proof-of-principle for decontamination of transplantation kidney through UV-C exposition of the perfusate solution
Authors: Goenaga-Mafud L. C. , Vollet-Filho J. D. , Costa C. , Inada N. M. , Netto A. S. , Kurachi C. , Bagnato V. S.
Published: March 2024

Kidney transplantation is a common yet highly demanding medical procedure worldwide, enhancing the quality of life for patients with chronic kidney disease. Despite its prevalence, the procedure faces a shortage of available organs, partly due to contamination by microorganisms, leading to significant organ disposal. This study proposes utilizing photonic techniques associated with organ support machines to prevent patient contamination during kidney transplantation. We implemented a decontamination system using ultraviolet-C (UV-C) irradiation on the preservation solution circulating through pigs' kidneys between harvest and implant. UV-C irradiation, alone or combined with ultrasound (US) and Ps80 detergent during ex-vivo swine organ perfusion in a Lifeport® Kidney Transporter machine, aimed to reduce microbiological load in both fluid and organ. Results show rapid fluid decontamination compared to microorganism release from the organ, with notable retention. By including Ps80 detergent at 0.5% during UV-C irradiation 3 log10 (CFU mL−1) of Staphylococcus aureus bacteria previously retained in the organ were successfully removed, indicating the technique's feasibility and effectiveness.
Modeling brain network flexibility in networks of coupled oscillators: a feasibility study
Authors: Chinichian Narges , Lindner Michael , Yanchuk Serhiy , Schwalger Tilo , Schöll Eckehard , Berner Rico
Published: March 2024

Modeling the functionality of the human brain is a major goal in neuroscience for which many powerful methodologies have been developed over the last decade. The impact of working memory and the associated brain regions on the brain dynamics is of particular interest due to their connection with many functions and malfunctions in the brain. In this context, the concept of brain flexibility has been developed for the characterization of brain functionality. We discuss emergence of brain flexibility that is commonly measured by the identification of changes in the cluster structure of co-active brain regions. We provide evidence that brain flexibility can be modeled by a system of coupled FitzHugh-Nagumo oscillators where the network structure is obtained from human brain Diffusion Tensor Imaging (DTI). Additionally, we propose a straightforward and computationally efficient alternative macroscopic measure, which is derived from the Pearson distance of functional brain matrices. This metric exhibits similarities to the established patterns of brain template flexibility that have been observed in prior investigations. Furthermore, we explore the significance of the brain’s network structure and the strength of connections between network nodes or brain regions associated with working memory in the observation of patterns in networks flexibility. This work enriches our understanding of the interplay between the structure and function of dynamic brain networks and proposes a modeling strategy to study brain flexibility.
Study on the influence of pore water pressure on shear mechanical properties and fracture surface morphology of sandstone
Authors: Cheng Jiaxin , Liu Yixin , Xu Chuanhua , Xu Jiang , Sun Mingzhi
Published: March 2024

To further investigate the weakening effect of pore water pressure on intact rock mechanics properties and characteristics of fracture surface after failure, direct shear tests of sandstone were conducted under different pore pressure. A 3D scanner was employed to digitize the morphology of the post-shear fracture surface. The variogram function was applied to quantify the anisotropic characteristics of post-shear fracture surface. The relationship between deformation during shear failure of intact rock and quantitative parameters of fracture surface after shear failure was initially established. It can be found that amplitudes of the sinusoidal surface determine the maximum value of variogram, and period affect lag distance that reach the maximum value of variogram. Test results revealed that the increase of pore pressure has obvious weakening effect on shear strength and deformation of rock. Moreover, the increase of pore pressure makes the shear fracture surface flatter. It can be obtained that both Sillmax and Rangemax are positively related to shear strain, but negatively related to normal strain.
Doppler ultrasonographic scan, gene expression and serum profile of immune, APPs and antioxidant markers in Egyptian buffalo–cows with clinical endometritis
Authors: El-Sayed Ahmed , Refaai Mohamed , Ateya Ahmed
Published: March 2024

The objective of this study was to elaborate Doppler ultrasonographic scan, genetic resistance and serum profile of markers associated with endometritis susceptibility in Egyptian buffalo–cows. The enrolled animals were designed as; twenty five apparently healthy buffalo–cows considered as a control group and twenty five infected buffalo with endometritis. There were significant (p < 0.05) increased of cervical diameter, endometrium thickness, uterine horn diameter, TAMEAN, TAMAX and blood flow through middle uterine artery with significant decrease of PI and RI values in endometritis buffalo–cows. Gene expression levels were considerably higher in endometritis-affected buffaloes than in resistant ones for the genes A2M, ADAMTS20, KCNT2, MAP3K4, MAPK14, FKBP5, FCAMR, TLR2, IRAK3, CCl2, EPHA4, and iNOS. The RXFP1, NDUFS5, TGF-β, SOD3, CAT, and GPX genes were expressed at substantially lower levels in endometritis-affected buffaloes. The PCR-DNA sequence verdicts of healthy and affected buffaloes revealed differences in the SNPs in the amplified DNA bases related to endometritis for the investigated genes. However, MAP3K4 elicited a monomorphic pattern. There was a significant decrease of red blood cells (RBCs) count, Hb and packed cell volume (PCV) with neutrophilia, lymphocytosis and monocytosis in endometritis group compared with healthy ones. The serum levels of Hp, SAA, Cp, IL-6, IL-10, TNF-α, NO and MDA were significantly (P˂0.05) increased, along with reduction of CAT, GPx, SOD and TAC in buffalo–cows with endometritis compared to healthy ones. The variability of Doppler ultrasonographic scan and studied genes alongside alterations in the serum profile of investigated markers could be a reference guide for limiting buffalo endometritis through selective breeding of natural resistant animals.
The impact understanding of exosome therapy in COVID-19 and preparations for the future approaches in dealing with infectious diseases and inflammation
Authors: Nasiri Zeynab , Soleimanjahi Hoorieh , Baheiraei Nafiseh , Hashemi Seyed Mahmoud , Pourkarim Mahmoud Reza
Published: March 2024

Cytokine storms, which result from an abrupt, acute surge in the circulating levels of different pro-inflammatory cytokines, are one of the complications associated with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. This study aimed to assess the effect of exosomes on the release of pro-inflammatory cytokines in patients with coronavirus disease 2019 (COVID-19) and compare it with a control group. The cytokines evaluated in this study were TNF-α, IL-6, IL-17, and IFN-γ. The study compared the levels of these pro-inflammatory cytokines in the peripheral blood mononuclear cells (PBMCs) of five COVID-19 patients in the intensive care unit, who were subjected to both inactivated SARS-CoV-2 and exosome therapy, with those of five healthy controls. The cytokine levels were quantified using the ELISA method. The collected data was analyzed in SPSS Version 26.0 and GraphPad Prism Version 9. According to the study findings, when PBMCs were exposed to inactivated SARS-CoV-2, pro-inflammatory cytokines increased in both patients and healthy controls. Notably, the cytokine levels were significantly elevated in the COVID-19 patients compared to the control group P-values were < 0.001, 0.001, 0.008, and 0.008 for TNF-α, IL-6, IL-17, and IFN-γ, respectively. Conversely, when both groups were exposed to exosomes, there was a marked reduction in the levels of pro-inflammatory cytokines. This suggests that exosome administration can effectively mitigate the hyperinflammation induced by COVID-19 by suppressing the production of pro-inflammatory cytokines in patients. These findings underscore the potential safety and efficacy of exosomes as a therapeutic strategy for COVID-19.
The soil microbiome of Lolium perenne L. depends on host genotype, is modified by nitrogen level and varies across season
Authors: Paina Cristiana , Fois Mattia , Asp Torben , Jensen Just , Hansen Pernille Bjarup , Rohde Palle Duun
Published: March 2024

Genotype by environment interactions (G × E) are frequently observed in herbage production. Understanding the underlying biological mechanisms is important for achieving stable and predictive outputs across production environments. The microbiome is gaining increasing attention as a significant contributing factor to G × E. Here, we focused on the soil microbiome of perennial ryegrass (Lolium perenne L.) grown under field conditions and investigated the soil microbiome variation across different ryegrass varieties to assess whether environmental factors, such as seasonality and nitrogen levels, affect the microbial community. We identified bacteria, archaea, and fungi operational taxonomic units (OTUs) and showed that seasonality and ryegrass variety were the two factors explaining the largest fraction of the soil microbiome diversity. The strong and significant variety-by-treatment-by-seasonal cut interaction for ryegrass dry matter was associated with the number of unique OTUs within each sample. We identified seven OTUs associated with ryegrass dry matter variation. An OTU belonging to the Solirubrobacterales (Thermoleophilales) order was associated with increased plant biomass, supporting the possibility of developing engineered microbiomes for increased plant yield. Our results indicate the importance of incorporating different layers of biological data, such as genomic and soil microbiome data to improve the prediction accuracy of plant phenotypes grown across heterogeneous environments.
Apigenin ameliorates genitourinary dysfunction in a type 1 diabetic rat model via Drp1 modulation
Authors: Khaled Mai , Salama Raghda A. M. , Aboughalia Azza , Tarek Mai , Mohamed Fawzy Nesma
Published: March 2024

The present study aimed to explore the potential ameliorative effect of apigenin (APG) against diabetes-associated genitourinary complications in rats. A diabetic rat model was induced by the intraperitoneal injection of streptozotocin (STZ). All experimental animals were treated with vehicle or vehicle plus APG at a dose of 0.78 mg/kg/day for 10 days, either once diabetes was confirmed or at the end of the 3rd week after confirmation of diabetes. Rats were sacrificed at the end of the fifth week. In addition to the histological assessment, an analysis of kidney function tests and serum testosterone was performed to assess diabetic genitourinary complications. Gene expression of the mitochondrial fission protein, dynamin related protein 1 (Drp1), was measured in renal and testicular tissues using qRT PCR. APG can increase body weight, reduce blood glucose levels, and improve renal and testicular functions in diabetic rats. APG decreased Drp1 overexpression in diabetic animals’ kidneys and testes. In summary, our current work discloses that APG attenuates diabetic genitourinary lesions in rats via suppressing Drp1 overexpression.