bluuebunny/crossref_metadata_2025 huggingface dataset:
ESTIMATION OF SEASONAL VERTICALLY INTEGRATED PRIMARY PRODUCTIVITY IN AMBON BAY USING THE DEPTH-RESOLVED, TIME-INTEGRATED PRODUCTION MODEL
Authors: Basit Abdul
Published: October 2018

Seasonal vertically integrated primary productivity (IPP) in eight stations of Inner Ambon Bay (IAB) and ninestations of Outer Ambon Bay (OAB) was estimated using the depth-resolved, time-integrated production model andvertical-a distribution model which was mainly dependent on chlorophyll-a profile. The profile was modeled as Gaussfunction in which its parameters were determined by minimizing the difference between the model and the averageof in situ chlorophyll-a concentration from 2008 to 2012. The data was collected by UPT BKBL-LIPI Ambonduring monitoring program in Ambon Bay. Based on this model, the results showed that the primary productivityduring the southeast monsoon was to some degree higher than that of other seasons. The main factors causing the higherIPP in this season were upwelling phenomenon and high rainfall intensity. From the results, it could be concludedthat run-off gave more impacts in IAB than in OAB while upwelling in Banda Sea affected the IPP more in OABthan that in IAB. Then, the existence of the sill located between IAB and OAB inhibited the mixing of IAB andOAB waters so that the levels of IPP and chlorophyll-a concentration in IAB were higher than that of in the OAB.
STATUS OF CORAL REEFS IN THE LEMBEH STRAITS AND ADJACENT WATER, NORTH SULAWESI
Authors: Suharsono -
Published: October 2018

Lembeh reefs and its water surrounding are among the richest and most diverse in marine biota in Indonesia. The biota and reefs in this area have an important contribution to the provinceâ€™s economy in the form of tourisms and fishery activities. An inventory using line intercept transect and free collection on the coral diversity has been conducted in four different locations showed that live coral cover ranging from 12.2â€“60.7% while sand rubble ranged from 4.67 to 78.67%. Among the life form categories, sponge dominated other biota with 0.47â€“21.97% coverage. Macro algae and turf algae cover were low at all sites, with average less than 2.07%. A total of 193 corals species belonging to 68 genera were recognized from this area. The differences among sites may be consequences of water quality, habitat variability and condition of the land base activities. Water turbidity in the coastal area of Kema was high due to the effects of water discharge from river mouth. Further, poor land management, development of port and human settlement leading to sedimentation, water pollution and poor water quality, remain the primary causes of reefs declines. Busy harbor and local sea transportation will be the major cause of coral reef degradation in the near future. There is a need to increase awareness of local people about the important of coral reefs and to develop management and conservation efforts involving scientists, academe and tourist operators. In addition, it is a need to strengthen the capabilities and capacities of institutions that concern reef management and enforce legislation.Â
BEHAVIOR OF ANOXIC WATER IN THE BANGPAKONG ESTUARY
Authors: Morimoto Akihiko
Published: October 2018

We carried out hydrographic observations in the Bangpakong estuary during transient period from rainy season to dry season in 2011 and 2012. The main objective of the hydrographic observations was to elucidate the behavior of anoxic water mass in the Bangpakong estuary, which was the possible cause of mass mortality of cultured shellfishes in the study area. We had succeeded the CTD and mooring observations in both years, and we had obtained timeâ€“series data of DO, salinity, and current velocity for approximately 2 months, which were the first long-term continuous records in terms of water quality in the Bangpakong estuary. From mooring data, it was revealed that DO and salinity at the bottom layer in aquaculture area oscillated with large amplitude, and the period of the oscillation corresponded to tidal variation. Amplitude of the oscillation was large in September and became small in October, and anoxic condition lasted for several days in October in 2011 and 2012. Current direction at station M1 in 2011 was opposite to that of in 2012 throughout the mooring period southward in 2011 and northward in 2012. Wind conditions in Chonburi in both years were almost the same, but river discharge in 2011 was much higer than that of in 2012. Therefore, it was possible that the difference of current direction at station M1 occurred due to change of buoyancy flux, and persistent period of anoxic condition was different between 2 observed years associated with circulation change. It was suggested that mass mortality of cultured shellfishes in Bangpakong estuary was caused by anoxic water because timing of mass mortality corresponded to that of anoxic water appearance qualitatively. It was also suggested that the anoxic water mass in aquaculture area was transported from off shore area by residual currents.
REPRODUCTION AND LARVAL REARING OF SANDFISH (Holothuria scabra)
Authors: Sembiring Sari Budi Moria , Wibawa Gigih Setia , Giri I Nyoman Adiasmara , Hutapea Jhon Harianto , Haryanti -
Published: October 2018

The natural stock of sea cucumber Holothuria scabra (known as sandfish) has been declining in both population and size, making it difficult to collect broodstock and juvenile sandfishes for aquaculture. This research aims to evaluate the reproduction of domesticated broodstock (F-1) and performance of the juveniles (F-2). Broodstock were reared in two rectangular concrete tanks with a dimension of 190 x 290 x 70 cm3. There were 12 individuals (7 males and 5 females) of broodstock (F-1) with total length and mean body weight of 12.0 ± 1.21 cm and 122.6 ± 32.37 g, respectively. Six broodstock were put in each tank and fed with compressed benthos at 4% biomass daily in the afternoon. During our experiment, domesticated sandfish broodstock successfully spawned twice. The first spawning (occurred in January 2017) had one female spawned that released 1,350,000 eggs with a hatching rate of 57.4%. Another broodstock spawned in April 2017 and produced 3,280,000 eggs with a hatching rate of 78.66%. The growth performance of 170 days-old juveniles (F-2) shows a total length of 5.66 ± 0.90 cm and a mean body weight of 10.08 ± 2.07 g. The survival rates are 5.19% and 8.68% for juveniles spawned in January and April, respectively. We conclude by showing that sandfish could be domesticated to produce seeds for further aquaculture development.
GÊNERO E RAÇA NA EDUCAÇÃO ESCOLAR: DIÁLOGOS COM DOCENTE POR UMA EDUCAÇÃO PARA DIVERSIDADE
Authors: Felipe Delton Aparecido , França Fabiane Freire
Published: October 2018

O objetivo deste artigo consiste em analisar os discursos de gênero e raça de professores e professoras da Educação Básica do estado do Paraná-Brasil. Os dados apresentados foram coletados durante a realização de grupos de conversas realizados com docentes entre os anos de 2008 e 2014. Caracterizamos esta análise como uma pesquisa ação participante que compreende de forma horizontal a relação entre o sujeito que ouve e o sujeito que fala. Como referencial teórico optamos pelos Estudos Culturais que possibilitaram a problematização e a desconstrução de discursos normativos e biologizantes. Os resultados da pesquisa apontaram a ocorrência de um movimento contínuo e conflituoso de opiniões acerca de gênero e raça por parte dos/as professores/as durante as discussões em grupo. Conclui-se que tais resultados propiciam entender a ancoragem dos discursos destes docentes em suas histórias de vida, seus valores e crenças pessoais.
A prática clínica em fitoterapia magistral: uma experiência interprofissional da Associação Brasileira de Fitoterapia
Authors: Fiut Maria Angélica , Deutsch Gabriela , Arruda Liziene , Marques Deborah , Leda Paulo Henrique , Botsaris Alex , Seixlack Antonio Carlos
Published: October 2018

Este artigo tem o objetivo de relatar a experiência interprofissional da prática clínica ambulatorial em fitoterapia magistral realizada pela Associação Brasileira de Fitoterapia (ABFIT) no Hospital Federal do Andaraí (HFA), a qual está inserida no programa de qualificação profissional desta associação. Trata-se de um estudo que incorpora relatos de experiência e levantamentos bibliográficos para a produção de dados. A prática clínica ambulatorial aqui relatada tem como base teórica a combinação de elementos médicos tradicionais e conhecimentos técnicos-científicos, em especial da Medicina Tradicional Chinesa. A integração desses fundamentos possibilita que a prescrição da fitoterapia seja executada através da associação de extratos vegetais em formulações magistrais que atendem à demanda individual do usuário. Esta abordagem permite ao aluno reunir indicações farmacológicas e tradicionais, proporcionando como resultado a maior eficácia do tratamento. Acredita-se que a experiência da ABFIT possa fornecer subsídios para o desenvolvimento de um modelo de atenção em saúde inovador no campo das Práticas Integrativas Complementares (PICs).
The Raw pH in Plants: A Multifaceted Parameter
Authors: Masoero Giorgio , Cugnetto Alberto
Published: October 2018

The measurement of the in vivo raw pH of vegetative organs is a unusual way obtaining plant knowledge. The authenticity of the pH parameter of the leaf and its independence from soil pH has already been highlighted. In the present work we observe how and to what extent water-temperature mechanisms as well as bio-fertilizers inocula can affect the raw pH and how great the biodiversity is in plants. A trial with Arabidopsis thaliana in a phytotrone has shown that, in the dark, the raw pH did not change from +18 to +35 °C (b = -0.0027 N.S.), while in the light, the regression coefficients were significant and negative, and the acidification in the leaves progressed from high (-0.0097) to normal (-0.0127) and then to low (-0.0370) water levels. We have confirmed that warming induces a decrease of raw petiole pH of -0.070 pH C°-1 in grapevine leaves. In accordance with water-temperature mechanisms, the raw pH in grapevines has been found to be significantly higher in well-watered plants (pH = 4.29) than in stressed ones (4.12), with a pH decay of -3.9%. On the other hand, an average reduction of 0.10 units of raw pH would signal an increase in water stress of about -0.59 Mpa. Among the phenomena that can influence the raw pH, we have outlined three biotic factors: i) acidification as a result of a symbiotic farming fertilization i.e through the use of mycorrhizal and microbial fertilizers, with an average decay of around -3%, as a probable signature of symbiosis; ii) an “acida plantarum natura” scenario over 49 species, ranging from pH 3.06 to 6.38 ; iii) a strong (R2= 0.9) inverse polynomial pseudo-relationship of the number of fungicide sprays on the raw pH in a set of 15 species. It is suggested that this simple new multifaceted parameter can deserve interest.
Dynamics of Adaptive Drive Equipment for Forming Billets Bricks
Authors: Purdyk Victor , Brytskiy Oleksandr , Sapognik Vitaliy
Published: October 2018

Abstract. In this paper, an option is provided for upgrading equipment for the formation of raw bricks by creating a compact design with an adaptive drive based on hydroautomatics. The basis of modernized construction is the further scheme of formation of executive bodies on the movement of workpieces. A mathematical model of equipment was developed that takes into account the peculiarities of the formation of forces of friction that arise during the movement in the executive power bodies and their inertia. As a result of the study of a mathematical model in the Matlab Simulink environment, the influence on the dynamics of motion of the main executive bodies of some parameters of the drive system is determined. It is noted that the dynamics of the drive is most affected by such parameters as the weight of moving executive bodies, the friction force is given, the hydraulic stiffness of the pressure line of the pumping station is given. The method of calculating the power of the pumping station of the adaptive drive was proposed, on the basis of which the design documentation was developed and the prototype of the equipment was manufactured.
Method of Determination of Technological Durabilityof Plastically Deformed Sheet Parts of Vehicles
Authors: Dragobetskii Volodymyr , Zagirnyak Mykhaylo , Naumova Olena , Shlyk Sergii , Shapoval Aleksandr
Published: October 2018

The purpose of the article is to develop an apparatus providing maximum or predicted durability of parts treated during their manufacture by plastic deformation. In these terms, the parameters of the technological process should provide the maximum or expected increase of the endurance limit in comparison with the initial parts values before the strengthening by the surface plastic deformation or after plastic forming. The article describes the influence of the degree of preliminary deformation on the kinetics of fatigue failure of metals and alloys. Experimental data of the ultimate deformations of welded workpieces were obtained, which make it possible to evaluate the manufacturing of parts with a weld seam at the design stage of the technological process. The developed method made it possible to determine the nonstationary field of stresses in the deformation region, ultimate deformations and the most rational scheme of the stress-strain state, which excludes the localization of deformations and destruction of the weld zone of a welded cylindrical workpieces.
PL-016 Endurance training, muscle fibre type composition and the maximal capacity for fat oxidation
Authors: Shaw Chris , Swinton Courtney , Morales Scholz Maria Gabriela , Erftemeyer Tasman , Aldous Andrew , Murphy Robyn , Howlett Kirsten
Published: October 2018

Objective A greater capacity for fat oxidation in endurance trained athletes is linked to greater utilisation of intramuscular lipid (IMCL). IMCL breakdown occurs only in type I muscle fibres yet little is known about the fibre type specific abundance of lipid regulatory proteins. We explored the impact of endurance training on the maximal rate fat oxidation, muscle fibre type and muscle fibre type specific abundance of proteins regulating IMCL metabolism.

Methods Endurance trained (n=7, 28 ± 3 years, VO2max62.6 ± 1.6 ml·min-1·kg-1) and untrained (n=8, 25 ± 1 years, VO2max44.9 ± 1.9 ml·min-1·kg-1) males performed an incremental exercise test to determine maximal fat oxidation rate. Muscle fibre type composition and fibre type-specific IMCL content was assessed with immunofluorescence microscopy and protein abundance was analysed with immunoblotting on pooled single muscle fibres and whole muscle.

Results Endurance trained individuals displayed a higher peak fat oxidation rate (0.49 ± 0.05 vs. 0.20 ± 0.03 g·min-1, P<0.05), which correlated with type I fibre percentage (R = 0.83, P < 0.01) and VO2max (R = 0.78, P < 0.01). Type I muscle fibres from endurance trained individuals had a greater abundance of ATGL. In whole muscle, the endurance trained group had greater abundance of PLIN2, PLIN5 and ATGL compared to the untrained group (P < 0.05). Furthermore, autophagy flux measured as LC3-II/I ratio was higher in type I muscle fibres and LC3-II/I, lysosomal markers (LAMP2) and chaperone-mediated autophagy markers (LAMP2A) were all higher in whole muscle of endurance trained individuals (P < 0.05).

Conclusions These results demonstrate that the maximal rate of fat oxidation is related to the proportion of type I muscle fibres. Furthermore, IMCL storage and the abundance of key proteins regulating lipid metabolism is fibre type specific and greater in endurance trained individuals. Muscle fibre type composition should be considered when investigating the regulation of IMCL utilisation and markers of autophagy.
A Metabonomic Study on the Urine of Rowing Athletes
Authors: Kang Yanyan , Liu Chengyi
Published: October 2018

Objective To extract information that affect sport performance, the technique of urine metabolism and quantitative difference（QD）analysis were combined in search of the characteristic metabolites of the rowing athletes.
Methods Morning urine were collected in three consecutive weeks, main peaks of the 1H NMR spectrum which have significant difference between the medalists and non-medalists were selected from thousands of one dimensional NMR hydrogen spectrum of urine. Pattern recognition method based on metabonomics combined with QD so that the metabolites which could reflect the competitive level of the elite athletes could be selected from the main components.
Results 1.The optimal principal component of rowing athletes were principal component 1 and 5（PC1&PC5）. 2. Results of 7-fold cross validation showed that the PLS-DA model were stable, reliable and has good prediction ability. Results of the repeatability experiment showed that sample test accuracy were above 85%. 3. N-methylnicotinamide was obtained by multi-criteria assessment methods as the characteristic metabolite. 4. Substance concentration related to aerobic and anaerobic metabolism were different in urine of the rowing athletes.
Conclusions The athletes' urine contains the information of sport performance. Metabonomics combined with QD analysis could be widely applied in the evaluation of rowing athletes' competitive ability. The role of nicotinic acid in regulating energy metabolism and protecting human body might be a necessary condition with which athletes could tolerance high strength training and competition.
OR-012 The Regulation of NF-κB-TNF-α/IDO/5-HT Axis by Aerobic Exercise against Hippocampal Neuroinflammation in CUMS Depressive Mice
Authors: Qu Honglin , Chen Jiaqin , Liu Ruilian , Chen Wei , Li Di , Peng QI
Published: October 2018

Objective To study the effect of aerobic exercise on the anti-chronic stress depression and the key metabolic enzymes indoleamine 2,3 peroxidase (IDO) of tryptophan and kynurenine pathway.

Methods  Adopt the method of random numbers to make depression modelling for mice with 1 or 2 kinds of stimulating factors for 28 days in view of the 13 kinds of chronic stress stimulation. Collect and analyse motionless time for FST and TST of mice by using the Noldus EthoVision XT9 system. Test the serum factor level of laboratory mice with Cusabio imported IDO, NF-ƙB and TNF-α kit. Make real-time fluorescent quantitative PCR verification of the mRNA expression and protein expression level of IDO, 5-HT, NF-ƙB and TNF-α in hippocampus.

Results After 4 weeks of chronic stress stimulation, the motionless time for FST and TST of mice in the Model Group obviously prolonged (p<0.05). The bioactivity of IDO, NF-ƙB and TNF-α in hippocampus increased. The mRNA expression of IDO, NF-ƙB and TNF-α in hippocampus increased, while the mRNA expression of 5-HT decreased (p<0.01). Aerobic exercise can shorten the motionless time of mice, inhibit the activity of IDO, NF-ƙB and TNF-α, reduce the mRNA expression quantity of IDO, NF-ƙB and TNF-α and enhance the expression of 5-HT.

Conclusions Aerobic exercise has an antidepressant effect on mice for chronic stress depression, which is related to the IDO activation induced by inhibit inflammatory cytokines. Aerobic exercise may inhibit the NF-ƙB to reduce the pathway of tryptophan and kynurenine, affect the direct and indirect induced effect of IDO, and adjust its activity and expression.
OR-026 Exercise induces HIF-1α redistribution in the small intestine
Authors: Wu Die , Cao Wei , Luo Beibei , Xiang Dao , Chen Peijie
Published: October 2018

Objective Intestinal epithelial cells are positioned between an anaerobic lumen and a highly metabolic lamina propria, affected by reduced blood flow and tissue hypoxia. Exercise induces blood flow redistribution, leading to hypoperfusion and gastrointestinal (GI) compromise. The hypoxia-inducible factor (HIF) 1α is pivotal in the transcriptional response to oxygen flux. In this study, we hypothesized that exercise induces GI system hypoxia and accumulates HIF-1α.

Methods (1) ROSA26 ODD-Luc/+ mouse model (ODD-Luc) was used to detect HIF-1α expression in the intestine (female, 8-week, n=6/group). ODD-Luc mice were randomized into 4 groups: stayed in 21% O2 as the normoxic control (C), exercise (E), injected HIF-1α inhibitor PX-478 before swimming (PS), placed in the chamber containing 9% O2 for 4 hours as the positive control (PC). (2) Exercise models were conducted by volume: Moderate Exercise (ME): mice voluntarily swam for 30 min; Heavy-intensity Exercise (HE): mice swam for 1.5 hours with 5% body weight loads attached to their tails; Long-time Exercise (LE): mice voluntarily swam for 3 hours or till fatigue.

Results (1) Exercise increased HIF-1α in the abdominal area. The luciferase activities boosted after exercise, compared to the controls (ME v.s. C, P<0.05; HE v.s. C, P<0.05; LE v.s. C, P<0.05) but no differences among three exercise groups (ME v.s. HE, P>0.99; ME v.s. LE, P>0.99; HE v.s. LE, P>0.99); (2) Exercise altered HIF-1α distribution in the small intestine in a time-dependent manner. The expression of HIF-1α was significantly increased after exercise and gradually reduced to the rest level. The photons increased at the 0th hour after exercise compared to that of the normoxic control (P<0.01). The level of photons then reduced over time, while the 2nd, 4th and 6th hour post-exercise were still greater than that of the normoxic control  (2nd hour v.s. C, P<0.01; 4th hour v.s. C, P<0.01; 6th hour v.s. C, P<0.05), and returned to normal after 24 hours (24th hour v.s. C, P>0.99).

Conclusions Exercise induced the distribution of HIF-1α in the small intestine. The expression of HIF-1α is shown in a time-dependent manner after exercise.
OR-041 Infection of Different Altitudes on College Students’ Body Shape
Authors: Qiu Xiaoxia , Zhao Jin
Published: October 2018

Objective Considering that guizhou is located in a plateau region with various gradient altitude,and we have never seen any relevant report on the study of different altitudes influence the students'physical fitness at home or abroad. the aim of this research is the author runs a normal physical fitness test on native 774 non-PE major students who all comes from different altitudes 774, so as to provide reference standard for promoting the health of the students group.

Methods literature data, fitness testing, statistic, and logic analysis.

Results 1、There was no obvious consistency between the height change of students in different atitudes indicating that the influence of different altitude environments on the height of students was not obvious.2、Most of the students on the plateau show the thickness of the skinfold is the thickest , while the thickness of the skinfold of students on the plain and the subplateau is different and irregular. 3. There was no significant difference in the chest circumference of the four groups of students at three altitudes, indicating that the environment at different altitudes had no significant influence on the development of the respiratory organs and chest muscles of the students. 4. The waist circumference of the three groups showed no obvious pattern, indicating that the environment at different altitude had no significant influence on the waist circumference. 5. The waist-hip range of female students on the plateau is relatively high, while other students are in the ideal range. 6. The BMI of all the students in the three places is within the range of 18.5 ~ 22.9, that is, all the students in the three places are in the normal range.

Conclusions altitude has no significant effect on students'height, chest circumference, waist circumference and BMI. The thickness of skinfold of students on plateau is thicker than that of plain and subplateau students. The waist-hip range of female students on the plateau is relatively high, while other students are in the ideal range.
OR-056 No cardiovascular responses to energy drink consumption in young healthy adult
Authors: Duan Rui , Liu Timon Cheng-Yi , Zhang Quan-Guang
Published: October 2018

Objective Svatikova et al. (2015) in JAMA have conducted a randomized trial of cardiovascular responses to energy drink consumption in healthy adults, and found it significantly increased levels of blood pressure and catecholamines in young healthy adults. Their data were re-analyzed in terms of fractal self-similarity and quantitative difference (QD) in this paper.

Methods 1. The logarithm to base golden section τ (lt) is called golden logarithm. It was found that σ=ltσ ≈ 0.710439287156503. 2. For a process from x1 to x2, lx(1,2)=lt(x2/x1) and its absolute vale are called the process logarithm and its QD, QDx(1,2). There are QD threshold values (αx,βx,γx) of function x which can be calculated in terms of σ. The function x is kept to be constant if QDx(1,2) < αx. A function in/far from its function-specific homeostasis is called a normal/dysfunctional function. A normal function can resist a disturbance under its threshold so that QDx(1,2) < βx. A dysfunctional function is defined as the QD is significant if βx ≦QDx(1,2) < γx and extraordinarily significant if QDx(1,2) ≧ γx. 3. Self-similarity was studied in the fractal literature: a pattern is self-similar if it does not vary with spatial or temporal scale. First-order self-similarity condition leads to the power law between two data sets A = {xi} and B = {yi}; yi = ai xi if the QDi of ai and the average of {ai} is smaller than βmin=min{βi} and the average QD of {QDi} is smaller than αmin=min{αi}. 4. The σ algorithm for integrative biology was established based on high-order self-similarity.

Results The 18 dimension data set consisted of all the 18 parameters. The first-order self-similarity held for the 18 dimension data sets between after and before for placebo or energy drink, and between placebo and energy drink for the 18 dimension ratio data set of after to before.

Conclusions There may be no cardiovascular responses to energy drink consumption in young healthy adult.
PO-047 Expression of Aromatase and Synthesis of Sex Steroid Hormones in Skeletal Muscle Following Exercise Training in Ovariectomized Rats
Authors: Shi Rengfei , Tian Xiangyang , Zhang Quanguang , Cheng Zepeng , Feng Yu
Published: October 2018

Objective Age-related muscle wasting (sarcopenia) is accompanied by a decrease in estrogen levels which can compromise the health of aging women. Recent studies have shown that the key enzyme of estrogen synthesis (aromatase) is detected in the skeletal muscle. The purpose of this study was to investigate the effects of exercise on the expression of aromatase and the synthesis of sex steroid hormones in skeletal muscle following exercise training.

Methods Fourteen female ovariectomized rats were divided into two groups, treadmill running (n=7) and sedentary (n=7) group. Exercise training on a treadmill (25 m/min, 60 min/day, 6 days/week) for 5 weeks. Immunofluorescence assay was used to detect estradiol and aromatase levels in soleus muscle and plantar muscle. Detected the expression of AKT, Aromatase, FoxO1, MyoD protein level by Western blotting.

Results We found that in ovariectomized rats, exercise training significantly increased the soleus and plantar muscles mass. The level of aromatase expression and 17-b-estradiol (E2) were increased significantly in skeletal muscle following exercise training(P < 0.05). In addition, the down-stream Akt-FoxO1-MyoD signaling pathway was significantly regulated in both soleus and plantaris muscles following exercise(P< 0.05).

Conclusions These results demonstrate that exercise training increased the expression of aromatase and local estrogen production in skeletal muscle, which potentially influences skeletal muscle in ovariectomized rats through activation of Akt-FoxO1-MyoD signaling pathway.
PO-063 Exercise alleviates insulin resistance by regulating MG53 and IR/IRS/AKT/mTOR signaling in db/db mice skeletal muscle
Authors: Zhang Yi , Ding Shuzhe , Sun Yi
Published: October 2018

Objective In the current study, we aim to investigate whether 12-week treadmill exercise alleviates insulin resistance and muscle atrophy, and to explore whether MG53 along with IR/IRS/AKT/mTOR cascade play a role in the physiopathological changes of db/db mice.

Methods 20 db/db mice and 20 age-matched non-diabetic m/m mice were assigned to 4 groups as MC (m/m control) group, ME (m/m exercise) group, DC (db/db control) group and DE (db/db exercise) group. After an intervention of treadmill exercise of moderate intensity for 12 weeks, glucose and insulin tolerance tests, insulin resistance index (HOMA-IR, homeostasis model assessment of insulin resistance) and lipid metabolic profile were determined using blood samples. Skeletal muscles were utilized for determination of cross-sectional area (CSA), protein level detection of MG53 and insulin signaling pathway.

Results Compared with MC mice, the AUC (areas under curve) of IPGTT (intraperitoneal glucose tolerance test) and IPITT (intraperitoneal insulin tolerance test) as well as HOMA-IR were significantly increased, and lipid parameters (serum triglyceride and total cholesterol) increased significantly in DC group. The upregulation of MG53 protein in different skeletal muscles (quadriceps, gastrocnemius and soleus muscle) could be observed in DC mice. Phosphorylated proteins of IR-β (β subunit of insulin receptor), IRS1, AKT (protein kinase B), mTOR (mammalian target of rapamycin), p70S6k and S6 ribosomal protein after acute insulin stimulation were downregulated with significance, whereas no significant difference was found in total protein levels of IR-β and AKT except IRS1 in DC group. The results of AUC of IPGTT and IPITT, HOMA-IR and serum lipid parameters in DE group were significantly decreased compared with DC group. 12-week moderate exercise was sufficient to downregulate the expression of MG53 in skeletal muscles of diabetic db/db mice. In addition, treadmill exercise-induced improvement of insulin signal transduction and insulin-dependent protein synthesis may partially account for the heavier muscle mass and larger muscle size.

Conclusions In summary, insulin resistance and muscle atrophy of diabetic db/db mice could be effectively attenuated by 12-week moderate treadmill exercise by regulating MG53, MG53-mediated ubiquitin-dependent degradation of IRS1 and insulin signaling transduction.
PO-098 Effect of HIIT on mitochondrial telomerase of skeletal muscle in aged rats
Authors: Liu Yiyi , Su Hao , Jiang Zhongye , Wen Tianhao , Shao Jia
Published: October 2018

Objective The HIIT and moderate-intensity exercise are two different exercise models among the public fitness. In recent years, HIIT become more and more popular, unfortunately, there is a tremendous lack of research being done effects of mitochondrial reverse transcriptase (TERT) on age-related degeneration of skeletal muscle by HIIT. The purpose of this study was to compare the HIIT group and moderate-intensity group, and research difference of telomerase expression and cardiopulmonary endurance between the exercise group and the quiet control group was discussed.

Methods  fifty-nine male Wistar rats were divided into three groups at random: control group (Q=19), moderate-intensity intervention group (M=20), and HIIT intervention group (H=20). The rats in Q group did not any exercise, and the rats in M group developed the exercise with 60% VO2max intensity for 8 weeks. H group did a training program for an 8-week exercise with alternating 40%, 60%, and 80% VO2max intensities. The rats in the experimental group were exercised for 50 minutes every day and trained for 5 days per week. After the baseline value group was sampled, each group of rats was selected after the training reached the specified number of weeks (4 and 8 weeks), and the maximum oxygen uptake test was performed before the material was taken. Single factor analysis of variance were used to assess differences in VO2max, and expression of protein between conditions.

Results It was found that H group VO2max was significantly higher than M group and Q group (P<0.05). At same time, the mTERT expression of the M group at the 4th week was significantly higher than that of the Q group (P<0.05). The mTERT expression in group H was significantly higher than that in group Q at week 8 (P<0.05).There was no significant difference between the H group and the Q group at 8th week (P<0.05).

Conclusions 1. HIIT exercise lasting for 8 weeks can effectively inhibit the decrease of maximal oxygen uptake in aging rats compared with moderate exercise. 2. HIIT training for 8 weeks promotes the expression of mTERT; 3. The maintenance of VO2max in aging rats may be related to the enhancement of mitochondrial antioxidant function by HIIT-promoted TERT to mitochondrial translocation.
PO-018 A Follow-up Study of 8-10 age pupil's physical activity status and related factor
Authors: Sun Qianli , Ren Hong
Published: October 2018

Objective Regular physical activity in children has many benefits，including reducing the occurrence of obesity and lower levels of cardiovascular disease risk factors, promote bone mass deposition, improve health. Our research in order to using accelerometer survey children daily PA status in our country, and analyzes its characteristic.

Methods Our research assessed the PA of the same group of children in Beijing yuxin Primary school for three consecutive years using ActiGraph accelerometers. The total available valid data are 183 children (male 94, female 89).

Results (1)The average total PA is 627.2±134.5 counts/min（boy accrued 665.6 ± 139.1 counts/min and girl 579.8 ± 112.2 counts/min ）, among them boy accrued 59.0min/d and girl 48.1min/d of MVPA. 35% of participants could meet the WHO PA guideline (boys =49%, girls = 18%).

(2) MVPA increased significantly from 8 to 9 years of age（MVPA of 8year-old children:53.2min/d,9year-old children:56.1min/d）and MVPA decline slightly from 9 to 10 (10 year-old children:55.8min/d).And both boys and girls meet this regular.

(3) Both in weekdays and weekend, the MVPA time of boys (60.2min/d,57.8min/d) are outstandingly higher than girls ( 49.7min/d ,46.5min/d).

(4) TWO-way (gender *BMI category) ANOVA revealed that girls were less active than boys.

(5) The moderate and vigorous physical activity time of obese children in weekends are 42.3 min/d while those of normal children are 49.7 min/d, (p<0.05).

(6) The total PA level, VPA, MVPA are negatively correlated with body fat percentage while LPA is positively correlated with BMI.

Conclusions Objective data indicated that Chinese youth are generally not physically active enough, and lower levels of PA were observed in girls, and during weekend days. The findings of this study will better inform the development of PA-related policies in schools, the design of PA intervention programs.
PO-024 Associations between the Sedentary Behavior and Cardiovascular Risk Factors in Adults of 40-49year
Authors: Lin Jiashi , Yang Xiaoyue , Yan Yi
Published: October 2018

Objective Large amounts of pieces of evidence suggest sedentary behavior(SB) might have a negative impact on all-cause mortality, cardiovascular disease, and metabolic system. Our aim is to explore the associations between sedentary behavior and cardiovascular risk factors in adults of 40-49year.

Methods Participants(N=372) were adults 40-49 years old who had a survey by using adult sit-Q-7d. The indexes of body mass index(BMI) and Waist circumference(WC) were measured as per standard protocols and blood pressure measured using an automated sphygmomanometer (Carescape V100; GE Healthcare, UK) following five minutes seated rest. HDL cholesterol (HDL-C), LDL cholesterol (LDL-C), triglycerides(TG) and fasting plasma glucose(FPG) were assessed for blood sampling of venous blood following an overnight fast (Cholestech LDX; Alere Inc, USA). We analyzed data in 2 h/day and 1 h/day as segmentation criteria of sitting time were classified as 8 groups(0<2, 2-4, 4-6, 6-8, 8-10, >10 h/day) and 10 groups(<1, 1-2, 2-3, 3-4, 4-5, 5-6, 6-7, 7-8, 8-9，>9 h/day). Statistical analyses were performed using STATA software 15.0(STATA Corp, College Station, TX). We performed multiple linear regression models adjusting for health-related confounders to assess the associations between SB of difference range and risk factors of cardiovascular disease.

Results (1)When the sitting time as category criteria in 2 h/day, the relationship of LDL-C(β=0.651，95%CI=0.069 to 1.232, P=0.028)  and HDL-C(β=0.129，95%CI=0.013 to 0.245, P=0.028)  to sitting time in segment of 6-8h/day were statistically significant after adjustment for relative factor of risk; and the same in TC(β=0.932，95%CI=0.244 to 1.620, P=0.008) to sitting time in segment of 8-10h/day; (2) When the sitting time as category criteria in 1 h/day, in 3-4h/day segment, the index of BMI(β=1.550，95%CI=0.025 to 3.074, P=0.046) had a significant rise; in 7-8h/day segment, the index of LDL-C(β=0.919，95%CI=0.155 to 1.683, P=0.019) also had a significant rise; as well as, in 8-9h/day segment, the index of TC(β=1.531，95%CI=0.497 to 2.565, P=0.004) had a highly significant. (3) As has mentioned above, the threshold for each segment of sitting time for the index of LDL-C in 7-8h/day segment and the index of TC in 8-9h/day.

Conclusions This study suggests that (1) the different category criteria of sitting time, for categories criteria in 1h/d or 2h/d, will help reduce the risk of Cardiovascular risk in some degree and determining to their dose-response relationships. (2) For the 40-49year adult of health, the sitting time threshold is less than 7 h/d, in order to decrease the detrimental effects on metabolic markers associated with cardiovascular disease and metabolic system. (3) As well as the sitting time threshold is less than 3 h/d for adding to the negative aging effect on risk of cardiovascular markers.
PO-034 Effect of Voluntary Exercise on Cartilage Morphology of Knee Osteoarthritis in Obese Mice Induced by High-fat Diet
Authors: Zong Wenhao , Liu Yang , Dai Lulu , Dong Guijun
Published: October 2018

Objective To examine the effect of voluntary wheel-running exercise on cartilage morphology of knee osteoarthritis(KOA) in obese mice induced by high-fat diet,and explore the protective role of 4 weeks voluntary wheel-running exercise on KOA,finally providing effective experimental evidence for clinical treatment of knee osteoarthritis.

Methods C57BL/6J mice were randomly assigned to the C-Sed group,C-Ex group,HF-Sed group and HF-Ex group.The control groups were fed a control diet(13.5% kcal from fat),and the high-fat groups were fed a high-fat diet(60% kcal from fat).After feeding 8 weeks different diets,the exercise groups were starting running.In order to examine the effect of voluntary wheel-running exercise on cartilage morphology of KOA,the joint of knee were harvested to be fixed,decalcified and embedded in paraffin,and the four-micrometer-thick sections were stained with both HE and toluidine blue .

Results After feeding twelve weeks different diets,the body mass of the high-fat diet group mice has a significant increase,which demonstrates that high-fat diet could successfully induce the mice obese.From the results of HE and toluidine blue,in comparison to the C-Sed group,the surface of the knee articular cartilage in the HF-Sed group was not intact and smooth,and the thickness of articular cartilage has a significant decrease（p<0.001）;contrary to the HF-Sed group,the surface of the knee articular cartilage in HF-Ex group was slightly smooth,and there was significant increase in cartilage thickness.

Conclusions Four weeks voluntary wheel-running exercise can increase cartilage thickness ,decrease the Mankin’s score and delay the degeneration of knee cartilage in obese mice.To conclude,the short-term wheel-running exercise protects against obesity-induced KOA.
PO-121 Influence of HDAC1 inhibitor on the E3-ligases expression in rat soleus during hindlimb unloading
Authors: Belova Svetlana , Mochalova Ekaterina , Shenkman Boris , Nemirovskaya Tatiana
Published: October 2018

Objective Muscle unloading leads to its atrophy development. The MuRF-1 and MAFbx E3-ligases expression is increasing under this condition. FOXO3 was considered to be the only transcription factor that triggers E3-ligases expression. Beharry A.W. et al pinpoints HDAC1 as a primary regulator of FoxO in skeletal muscle that is both sufficient and required for skeletal muscle atrophy. We aimed to determine the role of histone deacetylase 1 (HDAC1) proteins in activation of MuRF-1 and MAFbx E3-ligases expression at the early stage of muscle unloading.

Methods We investigate it by CI-994 (inhibitor of HDAC1) administration in male Wistar rats (180-200 g) upon 3-day hindlimb suspension. The method of hindlimb suspension was described in Morey-Holton E & Globus R (2002). 24 animals were divided into 3 groups (n=8 in each): C-control, CI - hindlimb suspension with CI-994 (i.p. 1 mg/kg/day), or placebo (HS group) administration. The animals were anaesthetized with an i.p. injection of tribromoethanol (240 mg/kg), soleus muscles were surgically excised, frozen in liquid nitrogen. The Western blot and RT-PCR analyzes were done. The statistical analysis was performed using REST 2009 v.2.0.12 and Bio-Rad CFX Manager programs at the significance level set at 0.05. The significant differences between groups were statistically analyzed using Mann-Whitney test.

Results The evaluation of the levels of mRNA expression of MuRF-1 and MAFbx showed that CI-994 treatment inhibited unloading induced up-regulation of MAFbx in CI group but had no effect on mRNA expression of MuRF-1. After unloading, mRNA expression of MAFbx increased 2.12-fold (p < 0.05) in HS group. There were statistically significant differences in MAFbx mRNA expression between HS and CI groups. When compared with the control, unloading increased MuRF-1 mRNA expression  1.67- and 1.56-fold in HS and CI groups, respectively. CI-994 treatment also inhibited unloading-induced upregulation of mRNA expression of ubiquitin. The levels of ubiquitin mRNA expression when compared with the control were 4.21- and 2.32-fold in HS and CI groups, respectively. We did not find any differences in the content of phosphorylated anabolic signaling system components (Akt/mTOR/S6k) between both suspended groups (CI and HS).

Conclusions Therefore, HDAC1 inhibiting prevented hindlimb suspension-induced up-regulation of MAFbx and ubiquitin, but did not any effect MuRF-1expression.

This work was supported by Russian Science Foundation (grant № 18-15-00062).
PO-127 Research progresses of exercise intervention methods in non- alcoholic fatty liver disease
Authors: Luo Chao , Li Xiangping , Liu Xinhua , Song Lihui , Wang Mei , Ge Jiaojiao
Published: October 2018

Objective This review summarizes the effects of aerobic exercise (AE), resistance exercise (RE), and high-intensity interval training (HIIT) on nonalcoholic fatty liver disease (NAFLD).Discuss The main differences of intervention methods, intervention time, and intervention effects among the NAFLD people, To explore the targeting and dose-response relationship of different exercise models intervention in NAFLD. 

Methods This paper uses the keywords such as Non-alcoholic fatty liver disease, non-alcoholic steatohepatitis, NAFLD, NASH, NAFLD and aerobic training, aerobic exercise, resistance training, resistance exercise, high-intensity interval training, high-intensity interval exercise, high-intensity aerobic exercise, HIAT, HIIT, HIT, etc to search in the web of science、Pubmed database, the deadline is March 1, 2018. Inclusion criteria: (1) study design: randomized controlled trials; (2) study questions: effects of exercise on hepatic steatosis in patients with NAFLD; (3) type of exercise: AE, RE, and HIIT; (4) subjects: biochemical examination liver biopsy or ultrasound examination includes a combination of abdominal imaging and magnetic resonance imaging (MRI) imaging of the NAFLD population. Exclusion criteria: (1) non-original studies (2) meeting abstracts (3) did not provide sufficient experimental data (4) animal experiments (5) non-English literature. The search results showed that a total of 16 studies reported the effects of exercise on NAFLD populations. 

Results (1) AE, RE and HIIT can reduce hepatic steatosis and improve liver histology in NAFLD people, but their intervention effects are different. AE stands out in reduce body weight,decreases insulin resistance (IR); RE stands out in reduce hepatic fat and increases muscle strength; HIIT has a significant effect in reducing hepatic fat and enhancing cardiovascular fitness. (2) the frequency, duration, and intervention period of AE and RE are similar; achieve the same or better intervention effect, HIIT only requires the 1/3 exercise time of the previous two.

Conclusions RE may be more effective than AE in patients with poor cardiovascular fitness, sarcopenia, and NAFLD who are unable to tolerate or participate in AE; HIIT has certain advantages in the time-effect and dose-effect due to less exercise time and smaller amount of exercise, This is easy for the NAFLD people to accept, and it will facilitate long-term adherence in the future.
PO-137 Epigenetic regulation of exercise-improved LTCC and BKCa channels function in hypertension mesenteric arteries
Authors: Zhang Yanyan , Shi Lijun
Published: October 2018

Objective To investigate the epigenetic mechanism of the voltage-gated L-type Ca2+ channel (LTCC) and the large-conductance Ca2+-activated K+ channel (BKCa) function in mesenteric arterial myocytes improved by regular aerobic exercise in hypertension.

Methods 12-week-old male SHR and WKY rats were randomly assigned to sedentary and exercise training groups, respectively. Exercise groups were performed a moderate-intensity treadmill running. After 8 weeks, patch clamp study, Ca2+ image, Western blot, qPCR, bisulfite sequencing PCR were used to detect the LTCC and BKCa channel currents, BKCa single channel gating properties, Ca2+ spark, mRNA and protein expression of LTCC α1c together with BKCa α and β1 subunits, DNA methylation level of α1c and β1 gene promoter region, miR-328 expression. In vitro experiment，miR-328 mimic and miR-328 inhibitor were transfected into cultured arterial myocytes to make miR-328 overexpressing or silencing, the mRNA and protein expression of α1c subunits were determined after 48 h transfection.

Results 1) After 8 weeks of exercise, SBP in both exercise groups of WKY and SHR were significantly lower than that of their sedentary counterparts. 2) Exercise normalized the increased LTCC and BKCa current density of mesenteric arterial myocytes in SHR. 3) Exercise attenuated the increased single BKCa channel open Probability (Po) and the amplitude of Ca2+ spark in hypertension. 4) Exercise inhibited the upregulated mRNA and protein expression of BKCa β1 subunit in mesenteric arteries from SHR; β1 gene promoter was demethylation in hypertension, exercise increased the methylation level at β1 gene promoter of SHR. 5) The protein expression of LTCC α1c subunit was significantly increased in SHR, while decreased by exercise; the expression of miR-328 in mesenteric arteries was highly negative correlation with α1c subunit. 6) The miR-328 overexpression by transfecting miR-328 mimic decreased α1c subunit protein level significantly, while miR-328 inhibitor made α1c subunit a slight increase.

Conclusions Regular aerobic exercise efficiently reduces blood pressure of SHR, enhances β1 gene promoter methylation, mediates miR-328 inhibiting the α1c expression at post-transcriptional level, which might be the epigenetic mechanism underlying exercise-improved LTCC and BKCa channels function in mesenteric arteries of hypertension.