bluuebunny/crossref_metadata_2025 huggingface dataset:
Estimating Carbon Sequestration Potential of Forest and Its Influencing Factors at Fine Spatial-Scales: A Case Study of Lushan City in Southern China
Authors: He Geng , Zhang Zhiduo , Zhu Qing , Wang Wei , Peng Wanting , Cai Yongli
Published: July 2022

Accurate prediction of forest carbon sequestration potential requires a comprehensive understanding of tree growth relationships. However, the studies for estimating carbon sequestration potential concerning tree growth relationships at fine spatial-scales have been limited. In this paper, we assessed the current carbon stock and predicted sequestration potential of Lushan City, where a region has rich vegetation types in southern China, by introducing parameters of diameter at breast height (DBH) and tree height in the method of coupling biomass expansion factor (BEF) and tree growth equation. The partial least squares regression (PLSR) was used to explore the role of combined condition factors (e.g., site, stand, climate) on carbon sequestration potential. The results showed that (1) in 2019, the total carbon stock of trees in Lushan City was 9.22 × 105 t, and the overall spatial distribution exhibited a decreasing tendency from northwest to south-central, and the carbon density increased with elevation; (2) By 2070, the carbon density of forest in Lushan City will reach a relatively stable state, and the carbon stock will continue to rise to 2.15 × 106 t, which is 2.33 times of the current level, indicating that Lushan forest will continue to serve as a carbon sink for the next fifty years; (3) Excluding the effect of tree growth, regional forest carbon sequestration potential was significantly influenced on site characteristics, which achieved the highest Variable Importance in Projection (VIP) value (2.19) for slope direction. Our study provided a better understanding of the relationships between forest growth and carbon sequestration potential at fine spatial-scales. The results regarding the condition factors and how their combination characteristics affect the potential for carbon sequestration could provide crucial insights for Chinese carbon policy and global carbon neutrality goals.
Using Ambulatory Care Sensitive Conditions to Assess Primary Health Care Performance during Disasters: A Systematic Review
Authors: Lamberti-Castronuovo Alessandro , Valente Martina , Aleni Chiara , Hubloue Ives , Ragazzoni Luca , Barone-Adesi Francesco
Published: July 2022

Ambulatory care sensitive conditions (ACSCs) are health conditions for which appropriate primary care intervention could prevent hospital admission. ACSC hospitalization rates are a well-established parameter for assessing the performance of primary health care (PHC). Although this indicator has been extensively used to monitor the performance of PHC systems in peacetime, its consideration during disasters has been neglected. The World Health Organization (WHO) has acknowledged the importance of PHC in guaranteeing continuity of care during and after a disaster for avoiding negative health outcomes. We conducted a systematic review to evaluate the extent and nature of research activity on the use of ACSCs during disasters, with an eye toward finding innovative ways to assess the level of PHC function at times of crisis. Online databases were searched to identify papers. A final list of nine publications was retrieved. The analysis of the reviewed articles confirmed that ACSCs can serve as a useful indicator of PHC performance during disasters, with several caveats that must be considered. The reviewed articles cover several disaster scenarios and a wide variety of methodologies showing the connection between ACSCs and health system performance. The strengths and weaknesses of using different methodologies are explored and recommendations are given for using ACSCs to assess PHC performance during disasters.
Adult and Elderly Risk Factors of Mortality in 23,614 Emergently Admitted Patients with Rectal or Rectosigmoid Junction Malignancy
Authors: Levy Lior , Smiley Abbas , Latifi Rifat
Published: July 2022

Background: Colorectal cancer, among which are malignant neoplasms of the rectum and rectosigmoid junction, is the fourth most common cancer cause of death globally. The goal of this study was to evaluate independent predictors of in-hospital mortality in adult and elderly patients undergoing emergency admission for malignant neoplasm of the rectum and rectosigmoid junction. Methods: Demographic and clinical data were obtained from the National Inpatient Sample (NIS), 2005–2014, to evaluate adult (age 18–64 years) and elderly (65+ years) patients with malignant neoplasm of the rectum and rectosigmoid junction who underwent emergency surgery. A multivariable logistic regression model with backward elimination process was used to identify the association of predictors and in-hospital mortality. Results: A total of 10,918 non-elderly adult and 12,696 elderly patients were included in this study. Their mean (standard deviation (SD)) age was 53 (8.5) and 77.5 (8) years, respectively. The odds ratios (95% confidence interval, P-value) of some of the pertinent risk factors for mortality for operated adults were 1.04 for time to operation (95%CI: 1.02–1.07, p < 0.001), 2.83 for respiratory diseases (95%CI: 2.02–3.98), and 1.93 for cardiac disease (95%CI: 1.39–2.70), among others. Hospital length of stay was a significant risk factor as well for elderly patients—OR: 1.02 (95%CI: 1.01–1.03, p = 0.002). Conclusions: In adult patients who underwent an operation, time to operation, respiratory diseases, and cardiac disease were some of the main risk factors of mortality. In patients who did not undergo a surgical procedure, malignant neoplasm of the rectosigmoid junction, respiratory disease, and fluid and electrolyte disorders were risk factors of mortality. In this patient group, hospital length of stay was only significant for elderly patients.
Advanced Oxidation Processes in Pharmaceutical Formulations: Photo-Fenton Degradation of Peptides and Proteins
Authors: Schöneich Christian
Published: July 2022

Formulations of therapeutic proteins are sensitive to photo-degradation by near UV and visible light. Mechanistically, especially the processes leading to protein modification under visible light exposure are not understood. Potentially, these processes may be triggered by a ligand to metal charge transfer in excipient-metal complexes. This article summarizes recent analytical and mechanistic work on such reactions under experimental conditions relevant to pharmaceutical formulations.
Polyoxometalate Nanoparticles as a Potential Glioblastoma Therapeutic via Lipid-Mediated Cell Death
Authors: Petronek Michael S. , Allen Bryan G. , Luthe Gregor , Stolwijk Jeffrey M.
Published: July 2022

Polyoxometalate nanoparticles (POMs) are a class of compounds made up of multiple transition metals linked together using oxygen atoms. POMs commonly include group 6 transition metals, with two of the most common forms using molybdenum and tungsten. POMs are suggested to exhibit antimicrobial effects. In this study, we developed two POM preparations to study anti-cancer activity. We found that Mo-POM (NH4)Mo7O24) and W-POM (H3PW12O40) have anti-cancer effects on glioblastoma cells. Both POMs induced morphological changes marked by membrane swelling and the presence of multinucleated cells that may indicate apoptosis induction along with impaired cell division. We also observed significant increases in lipid oxidation events, suggesting that POMs are redox-active and can catalyze detrimental oxidation events in glioblastoma cells. Here, we present preliminary indications that molybdenum polyoxometalate nanoparticles may act like ferrous iron to catalyze the oxidation of phospholipids. These preliminary results suggest that Mo-POMs (NH4)Mo7O24) and W-POMs (H3PW12O40) may warrant further investigation into their utility as adjunct cancer therapies.
Increasing Complexity of Ribosomes and Their Biogenesis
Authors: Lindahl Lasse
Published: July 2022

According to the classic ribosome model, developed in the 1960s and 1970s, its only function is to translate the four-letter nucleic acid code into the 20 amino acid peptide-code, while polymerizing amino acids into peptides with the help of a large complement of tRNAs and translation factors that cycle on and off the ribosome [...]
Structure–Activity Relationship of New Chimeric Analogs of Mastoparan from the Wasp Venom Paravespula lewisii
Authors: Ruczyński Jarosław , Parfianowicz Brygida , Mucha Piotr , Wiśniewska Katarzyna , Piechowicz Lidia , Rekowski Piotr
Published: July 2022

Mastoparan (MP) is an antimicrobial cationic tetradecapeptide with the primary structure INLKALAALAKKIL-NH2. This amphiphilic α-helical peptide was originally isolated from the venom of the wasp Paravespula lewisii. MP shows a variety of biological activities, such as inhibition of the growth of Gram-positive and Gram-negative bacteria, as well as hemolytic activity and activation of mast cell degranulation. Although MP appears to be toxic, studies have shown that its analogs have a potential therapeutic application as antimicrobial, antiviral and antitumor agents. In the present study we have designed and synthesized several new chimeric mastoparan analogs composed of MP and other biologically active peptides such as galanin, RNA III inhibiting peptide (RIP) or carrying benzimidazole derivatives attached to the ε-amino side group of Lys residue. Next, we compared their antimicrobial activity against three reference bacterial strains and conformational changes induced by membrane-mimic environments using circular dichroism (CD) spectroscopy. A comparative analysis of the relationship between the activity of peptides and the structure, as well as the calculated physicochemical parameters was also carried out. As a result of our structure–activity study, we have found two analogs of MP, MP-RIP and RIP-MP, with interesting properties. These two analogs exhibited a relatively high antibacterial activity against S. aureus compared to the other MP analogs, making them a potentially attractive target for further studies. Moreover, a comparative analysis of the relationship between peptide activity and structure, as well as the calculated physicochemical parameters, may provide information that may be useful in the design of new MP analogs.
Effects of Triterpene Soyasapogenol B from Arachis hypogaea (Peanut) on Differentiation, Mineralization, Autophagy, and Necroptosis in Pre-Osteoblasts
Authors: Yun Hyung-Mun , Lee Joon Yeop , Kim Soo Hyun , Kwon Il Keun , Park Kyung-Ran
Published: July 2022

Triterpenes are a diverse group of natural compounds found in plants. Soyasapogenol B (SoyB) from Arachis hypogaea (peanut) has various pharmacological properties. This study aimed to elucidate the pharmacological properties and mechanisms of SoyB in bone-forming cells. In the present study, 1–20 μM of SoyB showed no cell proliferation effects, whereas 30–100 μM of SoyB increased cell proliferation in MC3T3-E1 cells. Next, osteoblast differentiation was analyzed, and it was found that SoyB enhanced ALP staining and activity and bone mineralization. SoyB also induced RUNX2 expression in the nucleus with the increased phosphorylation of Smad1/5/8 and JNK2 during osteoblast differentiation. In addition, SoyB-mediated osteoblast differentiation was not associated with autophagy and necroptosis. Furthermore, SoyB increased the rate of cell migration and adhesion with the upregulation of MMP13 levels during osteoblast differentiation. The findings of this study provide new evidence that SoyB possesses biological effects in bone-forming cells and suggest a potentially beneficial role for peanut-based foods.
UBE2G1 Is a Critical Component of Immune Response to the Infection of Pseudomonas Plecoglossicida in Large Yellow Croaker (Larimichthys crocea)
Authors: Peng Jia , Li Wanbo , Wang Bi , Zhang Sen , Xiao Yao , Han Fang , Wang Zhiyong
Published: July 2022

The large yellow croaker (Larimichthys crocea) is one of the most economically valuable mariculture fish in China. Infection of Pseudomonas plecoglossicida can cause an outbreak of “internal organ white-spot disease”, which seriously affects the aquaculture of the large yellow croaker. Ubiquitylation is closely related to the post-translation modification of proteins and plays a vital role in many hosts’ immune defense pathways, while the E2-binding enzyme is a key factor in ubiquitination. Our previous genome-wide association study found that the ubiquitin-binding enzyme E2G1 (designed LcUbe2g1) was one of the candidate genes related to disease resistance in large yellow croaker. In this study, we analyzed the molecular characteristics, function, and immune mechanism of the LcUbe2g1. The full-length cDNA is 812 bp, with an open reading frame of 513 bp, encoding 170 amino acid residues. The results of the RT-qPCR and immunohistochemistry analysis revealed that its transcription and translation were significantly activated by the infection of P. plecoglossicida in large yellow croaker. Immunocytochemistry experiments verified the co-localization of LcUBE2G1 and the ubiquitin proteins in the head kidney cells of large yellow croaker. Through GST pull-down, we found that LcUBE2G1 interacted with NEDD8 to co-regulate the ubiquitination process. The above results indicate that LcUBE2G1 is essential in the regulation of ubiquitination against P. plecoglossicida infection in large yellow croaker, which lays a foundation for further study on the resistance mechanism of internal organ white-spot disease.
FOXM1 Is a Novel Molecular Target of AFP-Positive Hepatocellular Carcinoma Abrogated by Proteasome Inhibition
Authors: Li Ru , Okada Hikari , Yamashita Taro , Nio Kouki , Chen Han , Li Yingyi , Shimakami Tetsuro , Takatori Hajime , Arai Kuniaki , Sakai Yoshio , Yamashita Tatsuya , Mizukoshi Eishiro , Honda Masao , Kaneko Shuichi
Published: July 2022

Alpha-fetoprotein (AFP) is an oncofetal protein that is elevated in a subset of hepatocellular carcinoma (HCC) with poor prognosis, but the molecular target activated in AFP-positive HCC remains elusive. Here, we demonstrated that the transcription factor forkhead box M1 (FOXM1) is upregulated in AFP-positive HCC. We found that FOXM1 expression was highly elevated in approximately 40% of HCC cases, and FOXM1-high HCC was associated with high serum AFP levels, a high frequency of microscopic portal vein invasion, and poor prognosis. A transcriptome and pathway analysis revealed the activation of the mitotic cell cycle and the inactivation of mature hepatocyte metabolism function in FOXM1-high HCC. The knockdown of FOXM1 reduced AFP expression and induced G2/M cell cycle arrest. We further identified that the proteasome inhibitor carfilzomib attenuated FOXM1 protein expression and suppressed cell proliferation in AFP-positive HCC cells. Carfilzomib in combination with vascular endothelial growth factor receptor 2 (VEGFR2) blockade significantly prolonged survival by suppressing AFP-positive HCC growth in a subcutaneous tumor xenotransplantation model. These data indicated that FOXM1 plays a pivotal role in the proliferation of AFP-positive liver cancer cells. Carfilzomib can effectively inhibit FOXM1 expression to inhibit tumor growth and could be a novel therapeutic option in patients with AFP-positive HCC who receive anti-VEGFR2 antibodies.
Gut Microbiota—A Future Therapeutic Target for People with Non-Alcoholic Fatty Liver Disease: A Systematic Review
Authors: Forlano Roberta , Sivakumar Mathuri , Mullish Benjamin H. , Manousou Pinelopi
Published: July 2022

Non-alcoholic fatty liver disease (NAFLD) represents an increasing cause of liver disease, affecting one-third of the population worldwide. Despite many medications being in the pipeline to treat the condition, there is still no pharmaceutical agent licensed to treat the disease. As intestinal bacteria play a crucial role in the pathogenesis and progression of liver damage in patients with NAFLD, it has been suggested that manipulating the microbiome may represent a therapeutical option. In this review, we summarise the latest evidence supporting the manipulation of the intestinal microbiome as a potential therapy for treating liver disease in patients with NAFLD.
LncRNA H19 Impairs Chemo and Radiotherapy in Tumorigenesis
Authors: Garcia-Padilla Carlos , Lozano-Velasco Estefanía , Muñoz-Gallardo María del Mar , Castillo-Casas Juan Manuel , Caño-Carrillo Sheila , Martínez-Amaro Francisco José , García-López Virginio , Aránega Amelia , Franco Diego , García-Martínez Virginio , López-Sánchez Carmen
Published: July 2022

Various treatments based on drug administration and radiotherapy have been devoted to preventing, palliating, and defeating cancer, showing high efficiency against the progression of this disease. Recently, in this process, malignant cells have been found which are capable of triggering specific molecular mechanisms against current treatments, with negative consequences in the prognosis of the disease. It is therefore fundamental to understand the underlying mechanisms, including the genes—and their signaling pathway regulators—involved in the process, in order to fight tumor cells. Long non-coding RNAs, H19 in particular, have been revealed as powerful protective factors in various types of cancer. However, they have also evidenced their oncogenic role in multiple carcinomas, enhancing tumor cell proliferation, migration, and invasion. In this review, we analyze the role of lncRNA H19 impairing chemo and radiotherapy in tumorigenesis, including breast cancer, lung adenocarcinoma, glioma, and colorectal carcinoma.
Hypoxia Aggravates Inhibition of Alveolar Epithelial Na-Transport by Lipopolysaccharide-Stimulation of Alveolar Macrophages
Authors: Baloglu Emel , Velineni Kalpana , Ermis-Kaya Ezgi , Mairbäurl Heimo
Published: July 2022

Inflammation and hypoxia impair alveolar barrier tightness, inhibit Na- and fluid reabsorption, and cause edema. We tested whether stimulated alveolar macrophages affect alveolar Na-transport and whether hypoxia aggravates the effects of inflammation, and tested for involved signaling pathways. Primary rat alveolar type II cells (rA2) were co-cultured with rat alveolar macrophages (NR8383) or treated with NR8383-conditioned media after stimulation with lipopolysaccharide (LPS; 1 µg/mL) and exposed to normoxia and hypoxia (1.5% O2). LPS caused a fast, transient increase in TNFα and IL-6 mRNA in macrophages and a sustained increase in inducible nitric oxide synthase (NOS2) mRNA in macrophages and in rA2 cells resulting in elevated nitrite levels and secretion of TNF-α and IL-6 into culture media. In normoxia, 24 h of LPS treated NR8383 decreased the transepithelial electrical resistance (TEER) of co-cultures, of amiloride-sensitive short circuit current (ISCΔamil); whereas Na/K-ATPase activity was not affected. Inhibition was also seen with conditioned media from LPS-stimulated NR8383 on rA2, but was less pronounced after dialysis to remove small molecules and nitrite. The effect of LPS-stimulated macrophages on TEER and Na-transport was fully prevented by the iNOS-inhibitor L-NMMA applied to co-cultures and to rA2 mono-cultures. Hypoxia in combination with LPS-stimulated NR8383 totally abolished TEER and ISCΔamil. These results indicate that the LPS-stimulation of alveolar macrophages impairs alveolar epithelial Na-transport by NO-dependent mechanisms, where part of the NO is produced by rA2 induced by signals from LPS stimulated alveolar macrophages.
Polymeric Nanorepellent Systems Containing Geraniol and Icaridin Aimed at Repelling Aedes aegypti
Authors: de Andrade Lucas Rannier Melo , Guilger-Casagrande Mariana , Germano-Costa Tais , de Lima Renata
Published: July 2022

Repellents are among the leading products used against diseases transmitted by the Aedes aegypti mosquito. However, their indiscriminate use or high concentrations can cause severe adverse reactions, particularly in children and pregnant women. To protect them, nanotechnology is a promising tool to encapsulate active compounds against degradation, increase their effectiveness, and decrease their toxicity, as it can promote the modified release of the active compound. This study aimed to develop polymeric nanocapsules containing the repellent actives geraniol and icaridin using low concentrations of the active component, with the objective of promoting effective activity and greater safety against adverse reactions. The nanocapsules were developed by the interfacial deposition method, and the physicochemical properties of the nanocapsules were evaluated using dynamic light scattering (DLS), nanoparticle tracking analysis (NTA), zeta potential, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), release kinetics assay, and mathematical modeling. Cell viability was assessed by the MTT assay and genotoxicity analysis using the comet assay. The developed nanocapsules containing geraniol and icaridin showed mean diameters of 260 nm and 314 nm, respectively, with a polydispersity index < 0.2. The nanocapsules showed encapsulation efficiency values of 73.7 ± 0.1% for icaridin and 98.7 ± 0.1% for geraniol. Morphological analysis showed spherical nanocapsules with low polydispersity. The kinetic parameters calculated using the Korsmeyer–Peppas model indicated an anomalous release profile. Cell viability and genotoxicity analyses showed that the nanocapsules did not alter cell viability or damage DNA. The results demonstrate a promising nanostructured system with good physicochemical characteristics and good stability, with repellent activity against Aedes aegypti.
Natural and Synthetic Pyrethrins Act as Feeding Deterrents against the Black Blowfly, Phormia regina (Meigen)
Authors: Kojima Takeshi , Yamato Seiji , Kawamura Shinichi
Published: July 2022

Pyrethrum is a botanical insecticide derived from pyrethrum flowers. Feeding deterrence caused by pyrethrum has been reported in several sucking insects; however, there is no account of the cause of deterrence—whether from a single component or the combination of six active ingredients, called pyrethrins. We determined the feeding deterrence of natural pyrethrins, their two main components (pyrethrins I and II), and pyrethroid insecticides on the blowfly, Phormia regina. In a dual-choice feeding assay that minimized tarsal contact with food sources but allowed feeding through proboscises, natural pyrethrins, synthetic pyrethrins I/II, and allethrin were observed to induce deterrence at a concentration 16 times lower than the lowest concentration at which the knockdown rate increased. Feeding bouts were interrupted by intensive grooming of the proboscis at the deterring concentration, but no such grooming was observed to occur while feeding on the unpalatable tastants—NaCl, quinine, and tartaric acid. The underlying mode of action for the feeding deterrence of pyrethrins at sub-lethal concentrations probably occurs on the fly oral gustatory system, while differing from that of unpalatable tastants. The potent feeding deterrence of pyrethrins may provide effective protection for pyrethrum plants by rapidly deterring insects from feeding, before insecticidal activities occur.
Research and Development of Hybrid Power Units Heat Flow Diagrams with Cooled High-Temperature Steam Turbines
Authors: Rogalev Nikolay , Kharlamova Daria , Vegera Andrey , Naumov Vladimir , Karev Timofey
Published: July 2022

Fossil fuel thermal power plants account for almost 60% of Russian electricity and heat. Steam turbine units make almost 80% of this amount. The main method for steam turbine unit efficiency improvement is the increase in the initial steam parameters’ temperature and pressure. This reduces fossil fuel consumption and harmful emissions but requires the application of heat-resistant steel. The improvement in steel’s heat resistance leads to a non-linear price increase, and the larger the temperature increase, the more the steel costs. One of the methods of improving efficiency without a significant increase in the capital cost of equipment is an external combustion chamber. These allow an increase in the steam temperature outside the boiler without the need to use heat-resistant alloys for boiler superheaters and steam pipelines between the boiler and the steam turbine. The most promising is hydrogen–oxygen combustion chambers, which produce steam with high purity and parameters. To reduce the cost of high-temperature steam turbines, it is possible to use a cooling system with the supply of a steam coolant to the most thermally stressed elements. According to the calculations, the efficiency reduction of a power unit due to the turbine cooling is 0.6–1.27%. The steam superheating up to 720 °C in external combustion chambers instead of a boiler unit improves the unit efficiency by 0.27%. At the initial steam temperatures of 800 °C, 850 °C, and 900 °C, the unit efficiency reduction caused by cooling is 4.09–5.68%, 7.47–9.73%, and 8.28–10.04%, respectively.
Obesity and Body Mass Components Influence Exercise Tolerance and the Course of Hypertension in Perimenopausal Women
Authors: Bielecka-Dabrowa Agata , Gryglewska Katarzyna , Sakowicz Agata , Rybak Marek , Janikowski Kamil , Banach Maciej
Published: July 2022

The aim of this study was to identify the potential influence of obesity and body mass components on exercise tolerance assessed in cardiopulmonary exercise testing (CPET), biochemical and echocardiographic parameters and factors correlated with oxygen absorption at the anaerobic threshold in hypertensive women with low levels of physical activity in the perimenopausal period. The study comprised 188 hypertensive women divided, based on body mass index (BMI), into an obesity group and a non-obesity group. Women with BMI ≥ 30 kg/m2 had significantly higher parameters of left ventricular diastolic dysfunction in echocardiography, lower total body water (TBC) in percentage assessed by bioimpedance and significantly worse exercise capacity assessed by CPET. In the study group, VO2 AT (mL/kg/min) correlated positively with TBW (r = 0.4, p < 0.0001) and with the ratio of extracellular water to total body water (ECW/TBW) (r = 0.4, p < 0.00001) and negatively with fat (% and kg) (r = −0.4, p < 0.0001 for both). Obesity negatively affects parameters of diastolic left ventricular function, as well as exercise tolerance in CPET in hypertensive females during the perimenopausal period. The oxygen uptake at anaerobic threshold correlates positively with total body water and ECW/TBW and negatively with body fat; this connection is more pronounced in women without obesity. ClinicalTrials.gov Identifier: NCT04802369.
Does Board Cultural Diversity Contributed by Foreign Directors Improve Firm Performance? Evidence from Australia
Authors: Dodd Olga , Zheng Bowen
Published: July 2022

Australian firms hire an increasing number of foreign directors who bring various cultural perspectives to their boards’ conversations. We evaluate the effect of board cultural diversity contributed by foreign directors on firm performance for a sample of Australian companies, constituents of ASX200. We employ Hofstede’s six cultural dimensions to estimate board cultural diversity. We document a positive relationship between board cultural diversity and firm performance as measured by Tobin’s q and ROA after controlling for various board and firm characteristics. This suggests that more culturally diverse boards may bring benefits to their firms that outweigh the potential costs of conflict and miscommunication caused by cultural differences. Our finding holds after controlling for firm and time fixed effects, implementing an instrumental variable approach, controlling for a firm’s foreign operations and presence, and using alternative cultural diversity measures. We find that not all aspects of cultural differences matter, and it is the diversity in masculinity, uncertainty avoidance, and long-term orientation dimensions that positively determine firm performance. This finding on the positive effect of board cultural diversity for Australian firms contrasts with the evidence from other countries, highlighting that the value of cultural diversity can differ across countries and over time.
Coronary Vasospastic Angina: A Review of the Pathogenesis, Diagnosis, and Management
Authors: Rehan Rajan , Weaver James , Yong Andy
Published: July 2022

Vasospastic angina (VSA) is an under-appreciated cause of chest pain. It is characterised by transient vasoconstriction of the coronary arteries and plays a significant role in the pathogenesis of stable angina and acute coronary syndromes. Complex mechanistic pathways characterised by endothelial dysfunction and smooth muscle hypercontractility lead to a broad spectrum of clinical manifestations ranging from recurrent angina to fatal arrhythmias. Invasive provocation testing using intracoronary acetylcholine or ergonovine is considered the current gold standard for diagnosis, but there is a wide variation in protocols amongst different institutions. Conventional pharmacological therapy relies on calcium channel blockers and nitrates; however, refractory VSA has limited options. This review evaluates the pathophysiology, diagnostic challenges, and management strategies for VSA. We believe global efforts to standardise diagnostic and therapeutic guidelines will improve the outcomes for affected patients.
Ligand-Tuned Multi-Color Luminescence of Single Aluminum (III) Ion Atomic Centers and Their Selective Sensitivity to Different Metal Ions
Authors: Wang Qian , Li Longlong , Tang Qinglin , Liu Jin , Wang Yao , Wang Jiuxing , Kipper Matt J. , Xie Haijiao , Belfiore Laurence A. , Tang Jianguo
Published: July 2022

Achieving multi-color luminescence with a single atomic center in transition metal complexes is a challenge. In this work, luminescent materials with tunable emission properties were realized by complexation between aluminum (III) ions with the ligands 3-hydroxyflavone (3-HF) and 5,7-dichloro-8-hydroxyquinoline (DCHQ). Aluminum (III) complexes with a single ligand emitted blue from 3-HF and green from DCHQ. High quantum yields (QYs) of 29.42% and 37.00% were also obtained, respectively. DFT calculations revealed details of the photophysical properties of the complexes. Correspondingly, cyan light emission was obtained if these two complexes were mixed together, from which the emission wavelength was located at 470 nm and the QY was 20.52%, under 290 nm excitation. More importantly, the cyan light emitted by the mixtures had selective sensitivity to different metal ions, resulting in either quenching the fluorescence (in the case of Fe3+) or enhancing the fluorescence (in the case of In3+). The fluorescence enhancement effect of In3+ on metal complexes has not been previously reported, neither for transition metal nor lanthanide ions. The linear quenching behavior of Fe3+ functions in the 50–700 μM concentration range, and the linear enhancement behavior of In3+ is demonstrated in the 300–800 mM concentration range.
Predicting the Rheological Properties of Super-Plasticized Concrete Using Modeling Techniques
Authors: Amin Muhammad Nasir , Ahmad Ayaz , Khan Kaffayatullah , Ahmad Waqas , Ehsan Saqib , Alabdullah Anas Abdulalim
Published: July 2022

Interface yield stress (YS) and plastic viscosity (PV) have a significant impact on the pumpability of concrete mixes. This study is based on the application of predictive machine learning (PML) techniques to forecast the rheological properties of fresh concrete. The artificial neural network (NN) and random forest (R-F) PML approaches were introduced to anticipate the PV and YS of concrete. In comparison, the R-F model outperforms the NN model by giving the coefficient of determination (R2) values equal to 0.92 and 0.96 for PV and YS, respectively. In contrast, the model’s legitimacy was also verified by applying statistical checks and a k-fold cross validation approach. The mean absolute error, mean square error, and root mean square error values for R-F models by investigating the YS were noted as 30.36 Pa, 1141.76 Pa, and 33.79 Pa, respectively. Similarly, for the PV, these values were noted as 3.52 Pa·s, 16.48 Pa·s, and 4.06 Pa·s, respectively. However, by comparing these values with the NN’s model, they were found to be higher, which also gives confirmation of R-F’s high precision in terms of predicting the outcomes. A validation approach known as k-fold cross validation was also introduced to authenticate the precision of employed models. Moreover, the influence of the input parameters was also investigated with regard to predictions of PV and YS. The proposed study will be beneficial for the researchers and construction industries in terms of saving time, effort, and cost of a project.
Oxidation-Induced and Hydrothermal-Assisted Template-Free Synthesis of Mesoporous CeO2 for Adsorption of Acid Orange 7
Authors: Xu Yaohui , Ding Zhao
Published: July 2022

Hydrogen peroxide (H2O2), an accessible and eco-friendly oxidant, was employed for the template-free hydrothermal synthesis of mesoporous CeO2 based on a cerium carbonate precursor (Ce2(CO3)3•xH2O). Its microstructure and physicochemical properties were characterized by XRD, TEM and N2 sorption techniques. The formation of the CeO2 phase with a porous structure was strongly dependent on the presence of H2O2, while the values of the BET surface area, pore diameter and pore volume of CeO2 were generally related to the amount of H2O2 in the template-free hydrothermal synthesis. The BET surface area and pore volume of the mesoporous CeO2 synthesized hydrothermally at 180 °C with 10 mL H2O2 were 112.8 m2/g and 0.1436 cm3/g, respectively. The adsorption process had basically finished within 30 min, and the maximum adsorption efficiency within 30 min was 99.8% for the mesoporous CeO2 synthesized hydrothermally at 140 °C with 10 mL, when the initial AO7 concentration was 120 mg/L without pH preadjustment. The experimental data of AO7 adsorption were analyzed using the Langmuir and Freundlich isotherm modes. Moreover, the mesoporous CeO2 synthesized at 140 °C with 10 mL H2O2 was regenerated in successive adsorption–desorption cycles eight times without significant loss in adsorption capacity, suggesting that the as-synthesized mesoporous CeO2 in this work was suitable as an adsorbent for the efficient adsorption of AO7 dye from an aqueous solution.
Substituted Poly(Vinylphosphonate) Coatings of Magnetite Nanoparticles and Clusters
Authors: Bunge Alexander , Leoștean Cristian , Radu Teodora , Tripon Septimiu Cassian , Borodi Gheorghe , Turcu Rodica
Published: July 2022

Magnetite nanoparticles and clusters of nanoparticles have been of Increasing scientific interest in the past decades. In order to prepare nanoparticles and clusters that are stable in suspension, different coatings have been used. Phosphates and phosphonates are a preferred anchoring group for the coating of magnetite nanomaterials. However, poly(vinylphosphonates) have rarely been used as a coating agent for any nanoparticles. Here, poly(methylvinylphosphonate) and other substituted polyvinylphosphonates are described as new coatings for magnetite nanoparticles and clusters. They show great stability in aqueous suspension. This is also the first time phosphonate-coated magnetite clusters have been synthesized in a one-pot polyol reaction. The coated magnetite nanoparticles and clusters have been characterized by TEM, EDX, FTIR, magnetization measurement, XRD as well as XPS. It has been shown that substituted vinylphosphonates can be easily synthesized in one-step procedures and as a polymeric coating can imbue important properties such as stability in suspension, tight binding to the particle surface, the ability to be further functionalized or to tightly adsorb metal ions. For the synthesis of magnetite clusters the cluster formation, polymerization and coating are done in a one-pot reaction and the resulting magnetite clusters show a higher amount of phosphonate coating than with a three-step procedure including a ligand exchange.
Separation and Concentration of Astaxanthin and Lutein from Microalgae Liquid Extracts Using Magnetic Nanoparticles
Authors: Savvidou Maria G. , Tsiaka Thalia , Zoumpoulakis Panagiotis , Maggiorou Emanouella , Tyrovolas Konstantinos , Molino Antonio , Hristoforou Evangelos , Ferraro Angelo
Published: July 2022

The downstream processing of natural active molecules remains the most significant cost in the production pipeline. This considerable cost is largely attributed to rigorous chromatographic purification protocols. In an ongoing effort to abate the dependence on chromatography in downstream processing, alternative affinity matrices in the form of magnetic particles (e.g., iron oxide) have emerged as viable candidates. Nevertheless, biotechnological applications of iron oxide particles are still confined to the research level or for low-throughput clinical applications. Herein, we describe an efficient, quick, and environmentally friendly method for the isolation of astaxanthin and lutein, two carotenoids with very similar chemical structure, from extracts of the microalga Haematococcus pluvialis. The technology proposed, named Selective Magnetic Separation (SMS), is based on the use of magnetic materials carrying affinity ligands that bind carotenoids and is applied as second step of purification. The method, thanks to functionalized magnetic nanoparticles, reduces the use of organic or toxic solvents. In the present work, we examined the most efficient binding conditions such as temperature, magnetic nanoparticles concentration, and elution time, as well as their effects on carotenoids recovery, with the aim to improve the non-covalent binding between the ligand (amines) and astaxanthin/lutein. Our initial results clearly showed that it is possible to use magnetic separation as an alternative to chromatography to isolate important and valuable compounds.
Using the Theory of Functional Connections to Solve Boundary Value Geodesic Problems
Authors: Mortari Daniele
Published: July 2022

This study provides a least-squares-based numerical approach to estimate the boundary value geodesic trajectory and associated parametric velocity on curved surfaces. The approach is based on the Theory of Functional Connections, an analytical framework to perform functional interpolation. Numerical examples are provided for a set of two-dimensional quadrics, including ellipsoid, elliptic hyperboloid, elliptic paraboloid, hyperbolic paraboloid, torus, one-sheeted hyperboloid, Moëbius strips, as well as on a generic surface. The estimated geodesic solutions for the tested surfaces are obtained with residuals at the machine-error level. In principle, the proposed approach can be applied to solve boundary value problems in more complex scenarios, such as on Riemannian manifolds.
Cosmetic Potential of Pigments Extracts from the Marine Cyanobacterium Cyanobium sp.
Authors: Pagels Fernando , Almeida Cíntia , Vasconcelos Vitor , Guedes A. Catarina
Published: July 2022

The current mindset in the cosmetics market about sustainable ingredients had increased the search for new sources of natural active ingredients. Cyanobacteria are a great source of functional ingredients for cosmetics, as a producer of pigments with described bioactive potential (carotenoids and phycobiliproteins). This work aimed to evaluate the cosmetic potential of marine cyanobacterium Cyanobium sp. pigment-targeted extracts (carotenoids and phycobiliproteins), evaluating their in vitro safety through cytotoxicity assays, cosmetic-related enzyme inhibition, ingredient stability, and putative product (serum formulation). Results showed no cytotoxicity from the extracts in skin-related cell lines. Carotenoid extract showed anti-hyaluronidase capacity (IC50 = 108.74 ± 5.74 mg mL−1) and phycobiliprotein extract showed anti-hyaluronidase and anti-collagenase capacity (IC50 = 67.25 ± 1.18 and 582.82 ± 56.99 mg mL−1, respectively). Regarding ingredient and serum stability, both ingredients showed higher stability at low-temperature conditions, and it was possible to maintain the pigment content and bioactive capacity stable during the tested period, although in higher temperatures the product was degraded in a week. As a major conclusion, both extracts can be potential natural and sustainable ingredients for cosmetic uses, with relatively simple formulation and storage, and can be promising natural anti-aging ingredients due to their bioactive capacity.
Preventive and Therapeutic Effects of Krill Oil on Obesity and Obesity-Induced Metabolic Syndromes in High-Fat Diet-Fed Mice
Authors: Hwang Seung-Min , Kim Yeong Uk , Kim Jong-Kyu , Chun Yoon-Seok , Kwon Young-Sam , Ku Sae-Kwang , Song Chang-Hyun
Published: July 2022

Obesity increases the risks of metabolic syndromes including nonalcoholic fatty liver disease (NAFLD), diabetic dyslipidemia, and chronic kidney disease. Dietary krill oil (KO) has shown antioxidant and anti-inflammatory properties, thereby being a therapeutic potential for obesity-induced metabolic syndromes. Thus, the effects of KO on lipid metabolic alteration were examined in a high-fat diet (HFD)-fed mice model. The HFD model (n = 10 per group) received an oral gavage with distilled water as a control, metformin at 250 mg/kg, and KO at 400, 200, and 100 mg/kg for 12 weeks. The HFD-induced weight gain and fat deposition were significantly reduced in the KO treatments compared with the control. Blood levels were lower in parameters for NAFLD (e.g., alanine aminotransferase, and triglyceride), type 2 diabetes (e.g., glucose and insulin), and renal dysfunction (e.g., blood urea nitrogen and creatinine) by the KO treatments. The KO inhibited lipid synthesis through the modification of gene expressions in the liver and adipose tissues and adipokine-mediated pathways. Furthermore, KO showed hepatic antioxidant activities and glucose lowering effects. Histopathological analyses revealed that the KO ameliorated the hepatic steatosis, pancreatic endocrine/exocrine alteration, adipose tissue hypertrophy, and renal steatosis. These analyses suggest that KO may be promising for inhibiting obesity and metabolic syndromes.
Metagenome-Assembled Genome of Cyanocohniella sp. LLY from the Cyanosphere of Llayta, an Edible Andean Cyanobacterial Macrocolony
Authors: Vilo Claudia , Dong Qunfeng , Galetovic Alexandra , Gómez-Silva Benito
Published: July 2022

Cyanobacterial macrocolonies known as Llayta are found in Andean wetlands and have been consumed since pre-Columbian times in South America. Macrocolonies of filamentous cyanobacteria are niches for colonization by other microorganisms. However, the microbiome of edible Llayta has not been explored. Based on a culture-independent approach, we report the presence, identification, and metagenomic genome reconstruction of Cyanocohniella sp. LLY associated to Llayta trichomes. The assembled genome of strain LLY is now available for further inquiries and may be instrumental for taxonomic advances concerning this genus. All known members of the Cyanocohniella genus have been isolated from salty European habitats. A biogeographic gap for the Cyanocohniella genus is partially filled by the existence of strain LLY in Andes Mountains wetlands in South America as a new habitat. This is the first genome available for members of this genus. Genes involved in primary and secondary metabolism are described, providing new insights regarding the putative metabolic capabilities of Cyanocohniella sp. LLY.
On-Farm Practices Associated with Multi-Drug-Resistant Escherichia coli and Vibrio parahaemolyticus Derived from Cultured Fish
Authors: Dewi Rita Rosmala , Hassan Latiffah , Daud Hassan Mohammad , Matori Mohd. Fuad , Zakaria Zunita , Ahmad Nur Indah , Aziz Saleha A. , Jajere Saleh Mohammed
Published: July 2022

Aquaculture activities have been implicated as responsible for the emergence of antimicrobial resistance (AMR), leading to broad dissemination and transference of antibiotic resistance to pathogens that affect humans and animals. The current study investigates the on-farm practices and environmental risk factors that can potentially drive the development and emergence of multi-drug-resistant (MDR) Escherichia coli and Vibrio parahaemolyticus in the aquaculture system. A cross-sectional study was conducted on 19 red hybrid tilapia (Oreochromis spp.) and 13 Asian seabass (Lates calcarifer, Bloch 1970) farms on the west coast of peninsular Malaysia. Data were collected using a structured questionnaire pertaining to farm demography, on-farm management practices and environmental characteristics. Multi-drug-resistant E. coli (n = 249) and V. parahaemolyticus (n = 162) isolates were analyzed using multi-level binary logistic regression to identify important drivers for the occurrence and proliferation of the MDR bacteria. On-farm practices such as manuring the pond (OR = 4.5; 95% CI = 1.21–16.57) were significantly associated with the occurrence of MDR E. coli, while earthen ponds (OR = 8.2; 95% CI = 1.47–45.2) and human activity adjacent to the farm (OR = 4.6; 95% CI = 0.75–27.98) were associated with an increased likelihood of MDR V. parahaemolyticus. Considering the paucity of information on the drivers of AMR in the aquaculture production in this region, these findings indicate the targeted interventions implementable at aquaculture farms to efficiently abate the risk of MDR amongst bacteria that affect fish that are of public health importance.
Spectroscopic Studies of Mononuclear Molybdenum Enzyme Centers
Authors: Kirk Martin L. , Hille Russ
Published: July 2022

A concise review is provided of the contributions that various spectroscopic methods have made to our understanding of the physical and electronic structures of mononuclear molybdenum enzymes. Contributions to our understanding of the structure and function of each of the major families of these enzymes is considered, providing a perspective on how spectroscopy has impacted the field.
Actional Mechanisms of Active Ingredients in Functional Food Adlay for Human Health
Authors: Zeng Yawen , Yang Jiazhen , Chen Jia , Pu Xiaoying , Li Xia , Yang Xiaomeng , Yang Li’e , Ding Yumei , Nong Mingying , Zhang Shibao , He Jinbao
Published: July 2022

Medicinal and food homologous adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) plays an important role in natural products promoting human health. We demonstrated the systematic actional mechanism of functional ingredients in adlay to promote human health, based on the PubMed, CNKI, Google, and ISI Web of Science databases from 1988 to 2022. Adlay and its extracts are rich in 30 ingredients with more than 20 health effects based on human and animal or cell cultures: they are anti-cancer, anti-inflammation, anti-obesity, liver protective, anti-virus, gastroprotective, cardiovascular protective, anti-hypertension, heart disease preventive, melanogenesis inhibiting, anti-allergy, endocrine regulating, anti-diabetes, anti-cachexia, osteoporosis preventive, analgesic, neuroprotecting, suitable for the treatment of gout arthritis, life extending, anti-fungi, and detoxifying effects. Function components with anti-oxidants are rich in adlay. These results support the notion that adlay seeds may be one of the best functional foods and further reveal the action mechanism of six major functional ingredients (oils, polysaccharides, phenols, phytosterols, coixol, and resistant starch) for combating diseases. This review paper not only reveals the action mechanisms of adding adlay to the diet to overcome 17 human diseases, but also provides a scientific basis for the development of functional foods and drugs for the treatment of human diseases.