bluuebunny/crossref_metadata_2025 huggingface dataset:
DAMPAK POSITIF TIGER PARENTING TERHADAP KEMATANGAN EMOSIONAL ANAK (STUDI KASUS PADA SISWA SMA/SMK DI SOLO)
Authors: Ibrahim Kurnia , Nur Hidayah Novia , Fitri Annisa , Arum Mawarni Sekar , Khaffah Ramadhani Fissilmy
Published: July 2023

Tiger parenting is a term that is rarely known by the public where the parenting style that emphasizes children gets high academic achievement. This study was conducted to determine whether the Tiger Parenting style has a positive impact on children's emotional maturity. The research method used is qualitative research with a case study approach and data collection using questionnaires and interviews distributed by researchers. The subjects of this study were 3 high school/vocational high school students in Solo Raya who received the tiger parenting style. The results of this study explain that tiger parenting has a positive impact on the emotional maturity of high school/vocational high school children. This can be seen from how to channel emotions well, have an independent attitude, and accept input and criticism well. However, there are still aspects of social maturity that are lacking, namely social skills and empathy. This research can be used as a reference by parents to apply it but still have to adjust to the child's condition.
Keywords: emotional maturity; impact; tiger parenting
ANALISIS PERILAKU KEAGAMAAN MAHASISWA UIN RADEN MAS SAID SURAKARTA PENGGUNA APLIKASI TIKTOK
Authors: Yuliana Nur Rohmah Reva , Ida Mardiana Vivi , Ilmi Nurul , Tazkhiyatun Nuzulul , Wahyu Diana Nur Prajati Rilis
Published: July 2023

The TikTok application has many users, one of which is in Indonesia. Users consist of various groups, including students. Many students have accounts and are active in using the TikTok application. The use of the TikTok application by students has various impacts on student behavior. The purpose of this study was to determine the impact of the use of TikTok on the religious behavior of students at the State University of Raden Mas Said Surakarta. The method used in this study is a qualitative method while the data obtained from interviews, observations and observations. The use of the TikTok application by UIN Raden Mas Said Surakarta students has a positive and negative impact. The positive impact is when students watch content of knowledge, education, and da'wah. While the negative impact that occurs is the behavior of delaying prayer. The majority of students access the TikTok application only to get entertainment from the content presented.
Keywords: Impact; Religious Behavior; TikTok
PERAN ENGLISH FOR SPECIFIC PURPOSE (ESP) TERHADAP PERFORMA PEMBELAJARAN MAHASISWA AKUNTANSI
Authors: Ambar Sari Arum , Farida Luluk , Dwi Astuti Yuliana , Nurul Karimah Ulfa , Sarapil Hasria
Published: July 2023

This research aimed to identify the role of English for Specific Purpose (ESP) to the learning performance of the accounting students and the impact of understanding English on the performance of accounting students at UIN Raden Mas Said Surakarta. This research was conducted by using qualitative approach and descriptive method. The population of this study were accounting students and used random sampling for the sampling technique. The data collection was carried out by distributing questionnaires that were arranged in closed-ended and open-ended questionnaires. The research showed the importance of English for Specific Purpose (ESP) in accounting students, not only in improving their ability to understand the accounting courses, but also in improving their performance especially in making financial reports.
Keywords: Accounting Students; ESP; Learning
Determinants of school absences due to respiratory tract infections among children during COVID-19 pandemic: a cross-sectional study of the Sentinel Schools Network
Authors: Soriano-Arandes Antoni , Colom-Cadena Andreu , Bordas Anna , Ganem Fabiana , Alonso Lucia , Montoro Marcos , Gascon Mireia , Subirana Maria , Mas Ariadna , Sunyer Jordi , Palacín Pere Soler , Casabona Jordi
Published: July 2023

We aimed to assess the association of household and classroom
determinants with students’ school absence due to respiratory tract
infections (RTI) among 253 students (4-11 years) of 20 classrooms. We
collected 71 absences; RTI incidence was very high during the study
period, 17.5-33.1 cases per 100 population. Having someone else at home
with respiratory symptoms was the most significant epidemiological
factor (OR=9.12, CI 95%=2.54-33.39), suggesting that households are
crucial for the transmission of RTI to the children. A positive but not
statistically significant association was observed between higher median
levels of CO2 and respiratory-related absences (OR=1.2, CI
95%=0.98-1.46).
The Composition and Effects of a Young Children’s Environmental Education Program connecting to Family
Authors:   The Korea Associantion For Early Childhood Education Administration, Joe Mi-Jung , Kim Seung-Hee
Published: July 2023

The purpose of this study is to develop a young children’s environmental education program connecting to family and to investigate its effects. Researchers developed a young children’s environmental education program connecting to family according to 6-step process. The effects of the program were observed from thirty eight 5-year-old children (nineteen in experimental group, nineteen in comparative group) and their parents of S preschool in Gwangju metropolitan city. Experimental group participated in the young children’s environmental education program connecting. to family, and comparative group in daily activities according to the Nuri curriculum. The experiments were conducted ten times targeting to parents and twenty times targeting to children for ten weeks from the eighteenth of October to twenty-fourth of December in 2021. The results of this study were as follows. The young children’s environmental education program connecting to family was consisted of educational contents, teaching-learning methods, and evaluation to promote the environmental sensitivity and environmental preservation attitudes of children and parents. Also, the environmental sensitivity and environmental preservation attitudes of children and parents were increased meaningfully through the program. That is to say, the young children’s environmental education program connecting to family was effective to increase the environmental sensitivity and environmental preservation attitudes of children and parents. As a result, it is significant that this study suggests new model of a young children’s environmental education by showing the educational effects of the young children’s environmental education program connecting to family.
Outcome assessment of orthodontic clear aligner vs fixed appliance treatment in adolescents with moderate to severe malocclusions
Authors: Chou Byron , Nickel Jeffrey C. , Choi Dongseok , Garfinkle Judah S. , Freedman Howard M. , Iwasaki Laura R.
Published: July 2023

Objective

To compare the efficacy and efficiency of treatment with clear aligners (CAT) vs fixed appliances (FAT) in adolescents with Class I and II moderate to severe malocclusions.

Materials and Methods

One operator’s (Garfinkle) cases from 2014 to 2019, started at age 12–18 years, with pre- and posttreatment records were identified and used according to an institutional review board–approved protocol. Records were measured by two calibrated, blinded investigators, aided by software (OrthoCAD [Cadent, Fairview, N.J.], Dolphin Imaging & Management Solutions [Chatsworth, Calif]). Discrepancy index (DI) and cast radiograph evaluation (CRE) scores, treatment duration, number of scheduled and emergency visits, and reported appliance and interarch elastic wear compliance were compared between groups using Wilcoxon rank sum and Fisher’s exact tests. Cephalometric superimpositions were completed to evaluate craniofacial growth and dental changes.

Results

Records from 72 cases met the criteria and were included. For the 47 CAT and 25 FAT cases, mean DI (21 ± 5 and 24 ± 8, respectively; P = .20) and CRE (35 ± 10 and 34 ± 9, respectively; P = .90) scores were not significantly different. Other case attributes and reported appliance and interarch elastic wear compliance were also not significantly different. CAT vs FAT cases had significantly smaller treatment durations (24 ± 6 vs 27 ± 5 months; P = .01) and visit numbers (16 ± 5 vs 24 ± 4; P < .01), but emergency visit numbers were not significantly different (2 ± 2 vs 3 ± 2; P = .08).

Conclusions

In adolescents with Class I and II malocclusions and moderate to severe DI scores, on average, CAT vs FAT cases were completed 3 months faster with eight fewer visits, but treatment efficacy was not significantly different.
Antibacterial and antifungal properties of propylene glycol, hexylene glycol, and 1,3-butylene glycol in vitro.
Authors: Kinnunen T , Koskela M
Published: July 2023

The antimicrobial properties of three glycols, - propylene glycol, hexylene glycol, and 1,3-butylene glycol - against Candida albicans, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes A, Streptococcus mitis, and E. coli were studied in vitro. Within 20 h, 10% and 30% hexylene glycol in fresh tryptic soy broth were able to kill all the micro-organisms listed above. Five percent hexylene glycol showed some antimicrobial properties but the 1% agent had no effect. Thirty percent 1,3-butylene glycol and 30% propylene glycol were approximately as effective as 10% HG. The results speak in favour of using hexylene glycol in cosmetic and dermatological vehicles instead of propylene glycol and 1,3-butylene glycol.
Middermal elastolysis. Report of a case and immunohistochemical studies on the dermal distribution of fibrillin, vitronectin and amyloid P component.
Authors: Thörneby K , Sakai L , Dahlbäck K
Published: July 2023

A 39-year-old woman with demarcated wrinkled areas, histologically characterized by absence of elastic fibers in the middle and upper reticular dermis, is described. Immunoreactivity of vitronectin and amyloid P component, present at the periphery of elastic fibers in normal skin in adults, was absent from the middermis of lesional skin as were orcein stained fibers. C9 neoantigen immunoreactivity, associated with elastic fibers in sun-exposed skin of middle-aged and elderly individuals, was present in conjunction with elastic fibers in papillary and lower reticular dermis in lesional skin but was absent in the middermis. In contrast, a fibrillin immunoreactive network was present throughout the dermis, indicating that the elastin-associated microfibrils are retained in the absence of amorphous elastin in lesional skin of middermal elastolysis.
Skin response to histamine. Reproducibility study of the dry skin prick test method and of the evaluation of microvascular changes with laser Doppler flowmetry.
Authors: Van Neste D
Published: July 2023

The author presents original data obtained when using the dry skin prick test method to introduce histamine into the skin and by non-invasive evaluation of the skin blood flow changes at various sites/times during the development of the weal and flare reaction. The test method (1-second prick test duration and evaluation with laser Doppler flowmetry) generated reproducible responses when repeated in the same group of subjects (n = 10). At predefined fixed skin locations within the histamine-induced flare reaction area increased volumes of skin blood flow were recorded. When similar locations were explored after the control prick test, there were only minimal changes in skin blood flow. Pooled data recorded at four different sites located 1 cm from prick sites showed minimal variation and skin perfusion volumes were greater than basal values. The development of the histamine-dependent weal in its early phase (9th min) was associated with slightly smaller numbers of skin blood perfusion units, compared with recordings made during the 15th min. The control prick tests showed slightly higher levels at 9 min than at 15 min. This inverse relationship might be useful to quantify the histamine-specific changes in skin blood flow. These data also clearly illustrate that it is mandatory to state the precise place of measurement and time after challenge when reporting on instrumental evaluation of the skin response to histamine.
Minor skin surgery. Are prophylactic antibiotics ever needed for curettage?
Authors: Maurice P D , Parker S , Azadian B S , Cream J J
Published: July 2023

Curettage of skin lesions was not followed by bacteraemia in 22 patients. The risk of bacterial endocarditis after curettage and other minor skin surgery is small but should not be overlooked in those with a prosthetic heart valve, a history of other cardiac surgery, a previous episode of infective endocarditis, drug addiction, diabetes, alcoholism, immunosuppression, or renal failure--especially where the skin lesion might be infected.
Intercellular lamellar lipids in plantar stratum corneum.
Authors: Egelrud T , Lundström A
Published: July 2023

Plantar stratum corneum was examined by means of transmission electron microscopy after conventional osmium fixation and after fixation with ruthenium tetroxide. The latter fixative was used in order to reveal the possible existence of lamelarly ordered lipids in the intercellular space, as has previously been demonstrated for non-palmo-plantar stratum corneum. A major part of the plantar stratum corneum intercellular space was occupied by extracellular parts of desmosomes. In specimens fixed with ruthenium tetroxide the intercellular space not occupied by desmosomes was found to contain multiple alternating electron dense and electron lucid bands, suggestive of membraneous structures. This pattern appeared to be similar to that previously described for non-palmo-plantar stratum corneum. It is suggested that the intercellular lipids of palmo-plantar stratum corneum may be qualitatively similar to the intercellular lipids of non-palmo-plantar stratum corneum. The lower lipid content, expressed as weight per unit weight of tissue, in palmo-plantar stratum corneum as compared to non-palmo-plantar stratum corneum may be related to the fact that a larger portion of the intercellular space of the former tissue is occupied by desmosomes. The relatively high water permeability of palmo-plantar stratum corneum implies that desmosomes, i.e. non-lipid regions of the intercellular space, may have a high water permeability and hence could establish a hydrophilic route through the stratum corneum.
Long-term efficacy and tolerability of topical calcipotriol in psoriasis. Results of an open study.
Authors: Kragballe K , Fogh K , Søgaard H
Published: July 2023

Calcipotriol is a non-calcaemic vitamin D3 analogue. In short-term studies, topically applied calcipotriol is both efficacious and safe for the treatment of psoriasis vulgaris. The purpose of the present study was to determine the efficacy and safety of calcipotriol ointment in patients treated for approximately 6 months. Fifteen patients with plaque-type psoriasis were treated daily with calcipotriol ointment 50 micrograms/g. After treatment for 6 weeks there was a significant alleviation of erythema, infiltration and scaling. This degree of improvement was maintained throughout the study, except in one patient, who was withdrawn at week 15 because of minimal improvement. At the end of treatment, 80% of the patients showed a moderate improvement at least. Local adverse events occurred in 3 patients. These were mild and transient. Hypercalcaemia or other laboratory abnormalities did not develop in any patient. Morphometric examination of biopsies taken from perilesional skin (i.e. skin exposed to calcipotriol) at the end of treatment did not show signs of epidermal or dermal atrophy compared with uninvolved psoriatic skin. Although only a limited number of patients participated in the study, these results indicate that calcipotriol ointment 50 mu/g is both efficacious and safe for the long-term treatment of psoriasis.
The unexpectedly rapid response of fungal nail infection to short duration therapy.
Authors: Munro C S , Rees J L , Shuster S
Published: July 2023

To test our hypothesis that, by laying down a fungicidal barrier in the growing nail, a short course of antifungal therapy should be effective against onychomycosis, we treated 8 subjects with Trichophyton rubrum nail infection with terbinafine 125 mg b.d. for 14 days. All but one patient showed marked improvement, and 80% of fingernails and 37% of toenails were clinically cured after 6 months. Although this confirmed our prediction, the onset of response measured by outward movement of affected nail and negative cultures from distal nail clippings occurred after as little as 4 weeks. This was too soon for a fungicidal barrier to have grown out and indicates that the drug must have been carried directly into the diseased distal nail, presumably from newly formed ventral nail beneath it. The findings show that 1) short duration therapy, perhaps even a single dose, is possible in fungal nail infections 2) the ventral nail provides unexpectedly rapid access of drugs to the site of distal disease.
Langerhans' cell distribution in drug eruption.
Authors: Dascalu D I , Kletter Y , Baratz M , Brenner S
Published: July 2023

The number in and distribution of Langerhans' cells were studied in 11 patients with a maculopapular drug eruption. The Langerhans' cells (LC) were identified with a monoclonal antibody to OKT6 antigen, by employing an immunofluorescence technique. Skin biopsies were taken from lesional and non-lesional skin during the acute stage of the disease. LC in the lesional biopsies increased in number by 66% (p less than 0.001) and displayed more intense staining and more prominent dendrites than did LC from non-lesional skin. Control biopsies, taken from identical sites at least 4 weeks after the eruption disappeared, exhibited a cell distribution similar to the non-lesional acute stage (p = N.S.). Delivery of drugs via the circulation and their distribution into the skin may cause a type IV immune reaction due to LC activation by a drug-carrier complex.
Immediate and delayed hypersensitivity reactions to birch pollen in patients with atopic dermatitis.
Authors: Räsänen L , Reunala T , Lehto M , Virtanen E , Arvilommi H
Published: July 2023

We investigated immediate and delayed hypersensitivity to birch pollen in 10 patients with atopic dermatitis (AD) who had experienced a worsening of their eczema during the birch pollen season. The patients were prick- and patch-tested and antigen-induced basophil histamine release and lymphocyte proliferation were measured. 9/10 birch pollen-allergic patients proved positive in the histamine release test and the results correlated with specific IgE levels measured by RAST. Birch pollen antigen induced lymphocyte proliferation in 6/10 patients, but a positive patch test result was obtained in only one case. Both peripheral blood monocytes and purified epidermal Langerhans' cells were able to present birch pollen antigen to T cells, although Langerhans' s cells seemed to function less efficiently in this respect.
Significance of tubuloreticular structures in infants with neonatal lupus erythematosus and their mothers with Sjögren's syndrome.
Authors: Nitta Y , Ikeya T , Ohashi M
Published: July 2023

Four infants with neonatal lupus erythematosus who had annular erythema on the trunk and face reacted positively to tests for anti-Ro(SS-A)/La(SS-B) antibodies. Electron microscopic examination of vascular endothelial cells in the annular erythema revealed the presence of tubuloreticular structures. Once anti-Ro(SS-A)/La(SS-B) antibodies had turned negative, these tubuloreticular structures were no longer evident in the vascular endothelial cells in the same area where annular erythema had been present. Mothers of these 4 infants reacted positively to anti-Ro(SS-A)/La(SS-B) antibodies, but had no sicca syndrome. A biopsy taken from the minor salivary gland of the lip of each mother revealed marked periductal mononuclear cell infiltration. Primary subclinical Sjögren's syndrome was confirmed. Tubuloreticular structures were also observed in the vascular endothelial cells in the region of the minor salivary gland. These findings suggest that the presence of tubuloreticular structures may be related to anti-Ro(SS-A)/La (SS-B) antibodies.
Cell kinetics in skin disorders with disturbed keratinization.
Authors: Rijzewijk J J , Groenendal H , Van Erp P , Bauer F W , Van Vloten W A
Published: July 2023

A relatively simple immunohistochemical method was developed and used on cryostat sections. The monoclonal antibody Ki67 was used as marker for actively cycling cells and Pab601 for germinative cells. Counts were expressed as Ki67- or Pab601-positive cells/mm. In order to improve our understanding of the pathogenetic mechanisms in skin disorders with disturbed keratinization we have measured cell kinetic values in dyskeratosis follicularis, pemphigus benigna familiaris chronica, autosomal dominant ichthyosis vulgaris, X-linked recessive ichthyosis, atopic dermatitis and psoriasis and compared them with previous values derived with autoradiography using tritiated thymidine. The results showed that microscopical acanthosis is related to an increase of the germinative population, while the increased epidermal turnover is associated with increased numbers of cycling cells. The cell kinetic changes seem to be all secondary except in psoriasis where a dysregulation in the epidermal growth may cause the epidermal changes. This simple method allows quick evaluation of drug efficacy which might be useful in atopic dermatitis and psoriasis.
Binding and uptake of Trichophyton rubrum mannan by human epidermal keratinocytes: a time-course study.
Authors: Grando S A , Herron M J , Dahl M V , Nelson R D
Published: July 2023

Trichophyton rubrum infects skin. This fungus or its products might affect the function of epidermal cells. We previously reported that T. rubrum mannan (TRM) exhibits a suppressive effect on proliferation of human lymphocytes. The goal of the present study was to investigate the possibility of direct interaction of TRM with cultured normal human epidermal keratinocytes (EK). Mannan, a cell wall glycoprotein, was extracted from T. rubrum by precipitation with cetyltrimethylammonium bromide and conjugated with fluorescein isothiocyanate (FITC-TRM). After incubation of EK with 50 micrograms/ml FITC-TRM for 30 min, the surface of EK showed bright fluorescent staining. EK cultures pretreated with non-labelled TRM remained unstained. The fate of TRM bound to EK surface was determined in a time-course study. After pulse exposure to FITC-TRM, EK cultures were washed and incubated for various time periods. The EK moved surface mannan to the one area of cell membrane, so that at 4-6 h, the homogeneous staining of the entire cell surface was replaced by staining in a "cap" pattern. By 12 h, FITC-TRM was taken up into the cell, brought to the nuclear area and concentrated in the EK nucleoli. During the next 3 days nucleolar and cytoplasmic staining of the cells was observed. The intensity of fluorescence gradually diminished. On the 4th day, the sharp staining of organelles disappeared instead, a large number of small fluorescent granules were seen intra- and extracellularly. By the 6th day after exposure, no EK staining remained. Thus, EK specifically bound, internalized and apparently catabolized TRM.(ABSTRACT TRUNCATED AT 250 WORDS)
Clinical and non-invasive evaluation of 12% ammonium lactate emulsion for the treatment of dry skin in atopic and non-atopic subjects.
Authors: Vilaplana J , Coll J , Trullás C , Azón A , Pelejero C
Published: July 2023

Clinical dryness of the leg skin is a common problem among dermatological patients. The efficacy and safety of 12% ammonium lactate emulsion (Keratisdin) for the treatment of dry skin on the legs of atopic and non-atopic subjects has been assessed by clinical criteria and by five different non-invasive methods. These methods measure biophysical parameters such as electrical capacitance of stratum corneum, skin surface lipids, transepidermal water loss (TEWL), skin surface topography (scanning electron microscopy and image analysis) as well as the biomechanical properties of the skin. Treatment with the test emulsion significantly reduced the severity scores for dryness, desquamation and pruritus when measured 15 days later. All patients tested showed a significant increase in electrical capacitance, skin surface lipids, extensibility and firmness of the skin, and an improvement in the skin barrier function and skin surface topography. This study showed that non-invasive techniques are excellent complementary tools in clinical studies.
Topical ketoconazole does not potentiate oral cyclosporin A in allergic contact dermatitis.
Authors: McLelland J , Shuster S
Published: July 2023

Cyclosporin A is an effective drug but its use is limited by its side effects. Since oral ketoconazole inhibits the metabolism of oral cyclosporin, we set out to find out whether topical ketoconazole would enhance the effect in the skin of oral cyclosporin. Five patients with contact allergic dermatitis (CAD) were given a 6-day course of cyclosporin (1 mg/kg/day) and applied 2% ketoconazole cream to an area on one arm and the inert base to the other. Serial dilutions of the relevant allergen were applied to the arms at 3 days for 48 hours, and the responses were measured objectively a day later. There was no significant difference between responses at the two sites, indicating that topical ketoconazole does not enable the dose of oral cyclosporin to be reduced in CAD.
Roxithromycin in Lyme borreliosis: discrepant results of an in vitro and in vivo animal susceptibility study and a clinical trial in patients with erythema migrans.
Authors: Hansen K , Hovmark A , Lebech A M , Lebech K , Olsson I , Halkier-Sørensen L , Olsson E , Asbrink E
Published: July 2023

A new semisynthetic macrolide roxithromycin was evaluated for its potential use in the treatment of Lyme borreliosis. Using a macro-dilution broth technique, Borrelia burgdorferi was shown to be susceptible to roxithromycin with a minimal bactericidal concentration (MBC) of 0.06-0.25 microgram/ml. A systemic B. burgdorferi infection was established in gerbils a dosage of greater than or equal to 25 mg/kg/day roxithromycin for 10 days eliminated the infection. A single blind, randomized multicenter study was performed to evaluate the efficacy of roxithromycin 150 mg b.i.d. versus phenoxymethyl-penicillin 1 g b.i.d. for 10 days in patients with uncomplicated erythema migrans. The study was interrupted when 19 patients had enrolled because of five treatment failures. All 5 patients had received roxithromycin three patients had persisting or recurrent erythema migrans, one developed a secondary erythema migrans-like lesion and severe arthralgia and one developed neuroborreliosis. B. burgdorferi was isolated from skin biopsies after roxithromycin therapy from two patients with persistent erythema migrans and both isolates were still highly susceptible to roxithromycin (MBC = 0.03 microgram/ml). No treatment failures were seen in 10 patients treated with phenoxymethyl-penicillin. Roxithromycin is thus not recommended for treatment of Lyme borreliosis.
Digital verrucous fibroangioma: a new variant of verrucous hemangioma.
Authors: Kohda H , Narisawa Y
Published: July 2023

In this article, we report on 4 cases of a dome-shaped nodule on the dorsum of the finger, which had been present since birth and slowly enlarged. On light microscopic examination, these nodules showed similarities to verrucous hemangioma. However, they were characterized by distinct clinical features and proliferation of dermal connective tissue. We consider these tumors to be a variant of verrucous hemangioma and propose to term them digital verrucous fibroangioma.
Sequential concentration of chloroquine in human hair correlates with ingested dose and duration of therapy.
Authors: Runne U , Ochsendorf F R , Schmidt K , Raudonat H W
Published: July 2023

Human scalp hair was analyzed for chloroquine using gas-chromatography. In 5 patients it was demonstrated that the amount of uptake of chloroquine into the hair varied proportionally with the dosage (from 500 mg/week to 10 g single dose) and with the time of administration. The chloroquine concentrations ranged from 8 to 1100 micrograms/g hair. Chloroquine could be determined quantitatively after a single toxic dosage used in a suicidal attempt and also after low therapeutic doses. The sequential examination of the hair shaft allows an assessment of the chloroquine amount taken over time, the individual dosage, the initiation and termination of therapy. As hairs can be collected easily, they are a unique specimen for investigation, and it is suggested that they can virtually be used as a "tachogram" of chloroquine drug-therapy or intoxication.
Atypical presentation of co-existent Haemophilus ducreyi and Treponema pallidum infection in an HIV-positive male.
Authors: Abeck D , Eckert F , Korting H C
Published: July 2023

A 25-year-old homosexual black male presented with asymmetrical perianal ulceration of uncertain clinical origin. Indepth microbiological examination revealed the combined presence of Haemophilus ducreyi and Treponema pallidum. The atypical clinical appearance may have been due to the changed immunological status of the host's being infected with Human Immunodeficiency Virus.
The relation between lichen planus and hepatitis C: a case report.
Authors: Agner T , Fogh H , Weismann K
Published: July 2023

A case of simultaneous occurrence of lichen planus (LP) and hepatitis C in the same patient is presented. The patient had received treatment with interferon alpha for her chronic liver disease, and the association between LP, hepatitis C and interferon alpha treatment is discussed.
Secretion of urokinase and tissue-plasminogen activator by epidermal cells in the presence of psoriatic fibroblast-conditioned medium.
Authors: Horiuchi Y , Marshall J M , Cederholm-Williams S A , Ryan T J
Published: July 2023

The present study examined secretion of urokinase and tissue-plasminogen activator by epidermal cells in the presence of psoriatic or uninvolved skin fibroblast-conditioned medium. Using zymographic analyses, a 54kD lysis band and a small 110kD band derived from urokinase could be detected in the harvest fluid from keratinocytes treated with both psoriatic and uninvolved fibroblast-conditioned medium, as well as very weak lysis bands of 63kD and 120kD derived from tissue-plasminogen activator in the harvest fluid treated with psoriatic fibroblast-conditioned medium, but not with uninvolved fibroblast-conditioned medium.
Juvenile generalized pustular psoriasis in a pair of monozygotic twins presenting strikingly similar clinical courses.
Authors: Takematsu H , Rokugo M , Takahashi K , Tagami H
Published: July 2023

We describe an exceptionally rare case of juvenile generalized pustular psoriasis noted in monozygotic twins who, after developing the disease on the same day (the 48th day after birth) continued to show strikingly similar clinical features of generalized pustular psoriasis for 7 years. Not even therapeutic intervention by tonsillectomy performed at age 4 years on one of the twins, which was expected to have some beneficial effect, could decrease the number of attacks or pustulation compared with the counterpart.
One-minute dithranol therapy in psoriasis: a placebo-controlled paired comparative study.
Authors: Jekler J , Swanbeck G
Published: July 2023

In a double-blind left-right randomised comparison, 27 patients suffering from chronic plaque-type psoriasis vulgaris were treated for one minute with dithranol 2% ointment, Psoralon (Psoralon MT), on a selected psoriasis plaque on one half of the body and with a placebo ointment on a corresponding plaque on the other. The preparations were applied once daily for 8 weeks. Seventeen patients achieved clearing or considerable improvement with dithranol therapy, as compared with 6 patients with placebo (p = 0.002). Erythema, infiltration, scaling, pruritus and the overall result were assessed. Statistically significant differences in favour of dithranol treatment were seen for all five variables, except for pruritus. The average of these five variables, designated the mean score, was also analysed dithranol was seen to yield significantly better results (p = 0.001). Staining of clothes and the bathroom was noted by 3 and 5 patients, respectively, but no medical side effects were seen.
Treatment with bifonazole shampoo for seborrhea and seborrheic dermatitis: a randomized, double-blind study.
Authors: Segal R , David M , Ingber A , Lurie R , Sandbank M
Published: July 2023

Forty-four patients with seborrhea and seborrheic dermatitis of the scalp were treated with either bifonazole shampoo (22 patients) or the vehicle shampoo (22 patients) in a randomized, double-blind vehicle-controlled study. The patients were instructed to wash their scalps 3 times weekly for 6 weeks and were examined every 3 weeks. Responses were evaluated by clinicians using a clinical grading of scaling, erythema and overall improvement, and also by the patients, who assessed pruritus and overall improvement, using a scale of 0 to 3. The improvement following the bifonazole shampoo was found to be significantly greater than that achieved with the vehicle shampoo in regard to the clinical evaluation of scaling (p = 0.01) as well as patient evaluation of pruritus (p = 0.008) and overall improvement (p = 0.03). No major adverse side effects were recorded.
Intractable chronic furunculosis: prevention of recurrences with pentoxifylline.
Authors: Wahba-Yahav A V
Published: July 2023

A 60-year-old HIV-negative man with known noninsulin-dependent diabetes mellitus and glucose 6-phosphate-dehydrogenase deficiency anemia suffered from chronic recurrent furunculosis since the age of 30. In recent years, his condition had become increasingly severe and the recurrences increasingly frequent. Different measures including continuous therapy with large doses of systemic antibiotics for a period of 6 months failed to prevent the recurrences. Oral treatment with pentoxifylline 400 mg t.i.d. was prescribed, and 2 months later the patient experienced a dramatic and complete remission of his furunculosis. Six months later he was still totally free of lesions while continuing to take the same medication. Pentoxifylline may provide a new and effective approach to the previously difficult and often disappointing problem of the management of patients with chronic recurrent furunculosis.
Evaluation of port wine stain perfusion by laser Doppler imaging and thermography before and after argon laser treatment.
Authors: Troilius A , Wårdell K , Bornmyr S , Nilsson G E , Ljunggren B
Published: July 2023

Thirteen patients with port wine stains (PWS) were treated with argon laser therapy. Before and at different points in time following treatment, skin blood perfusion and temperature were mapped with laser Doppler imaging and thermography. In nine patients no elevation in blood perfusion was observed in the PWS in comparison with the surrounding normal skin before treatment. In the remaining four patients a significantly (p less than 0.01) higher blood flow was recorded within the PWS. Immediately after treatment nine patients showed elevated perfusion within the PWS. During the first two days following treatment, all patients showed a gradually decreasing hyperperfusion in the borderline between the PWS lesion and surrounding skin. Immediately after treatment 10 patients had a significantly (p less than 0.01) higher temperature in the PWS than in normal skin. During the first 24 h following treatment, an elevated perfusion was in general accompanied by a tissue temperature increase. Three and a half months after argon laser treatment, three patients showed excellent clinical results with no remaining PWS spots or scarring. Two of these patients had had both elevated perfusion and temperature in the PWS prior to treatment.
Treatment of psoriasis vulgaris with topical calcipotriol has no short/term effect on calcium or bone metabolism. A randomized, double/blind, placebo/controlled study.
Authors: Mortensen L , Kragballe K , Wegmann E , Schifter S , Risteli J , Charles P
Published: July 2023

The purpose of this double/blind, placebo/controlled study was to examine whether the vitamin D analogue calcipotriol, topically applied to psoriatic skin lesions, had any effect on calcium or bone metabolism. Thirty/four outpatients with psoriasis vulgaris were randomized to treatment with either calcipotriol ointment (50 micrograms/g) or vehicle ointment twice daily for 3 weeks. The patients were put on a calcium energy fixed diet and examined once weekly. The mean amount of calcipotriol ointment used was 40.3 g/week (range 8.2/95.4 g/week). The results of biochemical markers on calcium and bone metabolism showed no significant differences between the two groups. No correlation was found between the amount of ointment used and changes in parameters on calcium and bone metabolism during the 3/week treatment. It is concluded that calcipotriol ointment (50 micrograms/g), applied in doses of 8.2/95.4 g/week for 3 weeks to psoriatic skin lesions, has no effect on calcium or bone metabolism.
Levels of terbinafine in plasma, stratum corneum, dermis/epidermis (without stratum corneum), sebum, hair and nails during and after 250 mg terbinafine orally once per day for four weeks.
Authors: Faergemann J , Zehender H , Denouël J , Millerioux L
Published: July 2023

The distribution of terbinafine in stratum corneum dermis/epidermis (without stratum corneum), sebum, hair, nails and plasma was studied in human male volunteers during and after 250 mg orally once daily for 28 days. The highest concentration was seen in sebum, 56.07 micrograms/g, after 14 days of therapy. The concentration was still 1.0 microgram/g 44 days after stop of medication. In stratum corneum the highest concentration, 14.4 micrograms/g, was seen 1 day after the last day of therapy, and it was 2.1 micrograms/g 44 days after stop of medication. The concentrations in hair and nails were lower with a maximum of 2.36 and 0.39 micrograms/g respectively, 1 day after stop of therapy, and still 0.21 microgram/g in hair and 0.09 microgram/g in nails 55 days after the last day of medication. With the exception of nails, all other tissue levels were at all times above the plasma concentrations. For nails, tissue levels exceeded that of plasma as early as 1 day after stop of medication, and this difference continued to increase until the last day of tissue sampling, 55 days after the last tablet. These results indicate that terbinafine is delivered to the stratum corneum through sebum and to a minor extent by direct diffusion through dermis/epidermis. Probably short/term therapy with terbinafine may be effective in the treatment of several dermatomycoses, due to the strong binding of terbinafine to stratum corneum for a long time after stop of medication.(ABSTRACT TRUNCATED AT 250 WORDS)
alpha/Amino/n/butyric acid methyl ester induces concentrative uptake of L/dopa in human Langerhans' cells normally not operative for L/dopa transport.
Authors: Falck B , Andersson L , Mikulowska A , Ronquist G
Published: July 2023

We recently reported the existence of two kinds of human epidermal Langerhans' cells (LC), one which can take up and accumulate L/dopa and one which cannot. The dopa(+) LC take up L/dopa by carrier/mediated exchange diffusion, that is, the influx of L/dopa and the outflow of an intracellular substance are linked via the same carrier. The nature of the fundamental difference between L/dopa(+) and L/dopa(/) cells has not been clarified. We have now found that alpha/amino/n/butyric acid methyl ester (ABA/OME) penetrates into intracellular compartments, perhaps endosomes or lysosomes, of all LC, where hydrolysis results in the accumulation of the free amino acid (ABA). This accumulation causes a considerable increase in osmotic pressure of the membrane/limited organelle, leading to influx of water and swelling. Co/incubation with L/dopa revealed an influx of L/dopa into LC which normally cannot take up this amino acid. It is suggested that these LC lack the capacity to synthesize and/or store the counterpart which allows L/dopa to enter the dopa(+) LC, but that ABA in the L/dopa(/) LC can function as an equivalent counterpart.
Adverse immune reactions to gold in rheumatoid arthritis: lack of skin reactivity.
Authors: Goldermann R , Schuppe H C , Gleichmann E , Kind P , Merk H , Rau R , Goerz G
Published: July 2023

Adverse immune reactions develop in up to 30% of patients treated with gold compounds. However, sensitization to gold(I) drugs is rarely demonstrated by in vivo or in vitro testing. Recent data from a mouse model provides evidence that gold(I) is oxidized to gold(III) before T cells are sensitized. To study the diagnostic value of skin tests, patch testing with various gold compounds / including gold(I) and gold(III) / was performed in 50 patients with rheumatoid arthritis treated with gold(I) drugs. Positive patch test reactions to either gold(I) or gold(III) compounds were not detected. In contrast, the lymphocyte transformation test (LTT) revealed a gold(III)/induced response in one of the 7 patients being tested. We conclude that patch testing fails to indicate T cell sensitization to gold(I) drugs in rheumatoid arthritis patients. The in vitro response to gold(III) obtained by LTT supports the hypothesis that biooxidation of gold(I) compounds may play a crucial role for sensitization.
Pemphigus vulgaris associated with acquired hemophilia A due to factor VIII inhibitor.
Authors: Ishikawa O , Tamura A , Ohnishi K , Miyachi Y
Published: July 2023

A rare case of pemphigus vulgaris associated with acquired hemophilia A is reported. The presence of factor VIII inhibitor is confirmed, and the co/existence of two autoimmune diseases is discussed.
Evidence of increased keratinocyte proliferation in air/liquid interface cultures of non/bullous congenital ichthyosiform erythroderma.
Authors: Amsellem C , Haftek M , Hoyo E , Thivolet J , Schmitt D
Published: July 2023

Modern pharmacological and dermatological research requires the use of appropriate in vitro models which permit a faithful reproduction of various aspects of the in situ situation. The air/exposed culture of keratinocytes on dead de/epidermized dermis is one of the best models of in vitro epidermal differentiation known at the moment. In this study, we verified the model's validity for the reproduction of a hyperproliferative genodermatosis: non/bullous congenital ichthyosiform erythroderma. We used subcultured epidermal keratinocytes originating from normal and ichthyotic patients. Light and electron microscopy of pathological cultures disclosed, on day 14, a terminally differentiated epidermis with a marked granular layer and hyperkeratosis which, however, was not dramatically different from the normal controls. On day 25, the normal cultures displayed an even more pronounced hyperkeratosis and hypergranulosis, whereas the reconstructed epidermis of pathological origin presented a considerable reduction of the viable non/keratinized compartment and a focal parakeratosis. Indirect immunofluorescence revealed the expression of several differentiation markers which were not observed in the immersed culture models (e.g. the desmosome/ and differentiation/related antigens KM48 and G36/19). Abundant keratohyalin granules were stained with AKH1 antibody and observed even in the deep epidermal layers, but no profilaggrin/filaggrin conversion could be detected biochemically.(ABSTRACT TRUNCATED AT 250 WORDS)
Use of scale antibodies for the detection of antigens in psoriatic lesions.
Authors: Iversen O J , Bergh K , Lysvand H
Published: July 2023

Extractable IgG from psoriatic scale was purified, labelled with biotin and used in ELISA and immunofluorescence (IF) in an attempt to detect and localize prominent antigens in psoriatic scale extracts and in psoriatic lesions, respectively. Biotinylated immunoglobulins isolated from psoriatic scale from each of 5 patients were used. Scale extracts were fractionated on a Sephacryl S/300 column, and antigens detected by scale IgG were eluted in the void volume and at a Kav 0.55. The profile was very similar for each antibody preparation. Antigens recognized by serum IgGs from both healthy controls and psoriatic patients were detected in the void volume only. Antigens recognized by a rabbit antiserum against the psoriasis/associated antigen, pso p27 (6), were restricted to the fractions eluted at a Kav 0.55. Furthermore, the binding of scale IgG to the antigens eluted at Kav 0.55 was inhibited by purified pso p27 antigen. Two of the scale antibody preparations gave rise to a distinct fluorescence on skin biopsies from psoriatic lesions in indirect immunofluorescence. The antigens recognized were localized to a subfraction of dermal cells and in the endothelial lining of some of the dermal vessels. Double labelling with these scale antibodies and a rabbit anti/pso p27 antiserum showed that both antibody preparations bound to the same cells in the psoriatic lesions, while only a minority of these cells were recognized by a murine monoclonal antibody against human IgG. The observations described indicate that the pso p27 is a major antigen in the immune reactions in psoriasis.
Keratinocyte proliferation in epidermal keratinocyte disorders evaluated through PCNA/cyclin immunolabelling and AgNOR counting.
Authors: Kanitakis J , Hoyo E , Chouvet B , Thivolet J , Faure M , Claudy A
Published: July 2023

The assessment of cell proliferation is important to our understanding of hyperproliferative disorders. In this work we evaluated the proliferation characteristics of epidermal keratinocytes in diseases with abnormal keratinization by two different methods (immunostaining for the proliferating cell nuclear antigen//PCNA and histochemical staining for nucleolar organizer region//associated argyrophilic proteins//AgNORs). Twenty/seven specimens from diseases with an abnormal keratinization were studied and compared with specimens of normal human skin. As compared with the latter, the numbers of PCNA/positive epidermal keratinocytes were increased in psoriasis, congenital non/bullous ichthyosiform erythroderma, epidermolytic hyperkeratosis and chronic dermatitis and decreased in ichthyosis vulgaris, X/linked ichthyosis and pityriasis rubra pilaris. In most cases a parallel modification of AgNORs was found. We conclude that although PCNA immunolabelling and AgNOR staining do not provide strictly correlated values, both appear as useful markers for the assessment of keratinocyte proliferation in epidermal disorders.
Duration of analgesia following application of eutectic mixture of local anaesthetics (EMLA) on genital mucosa.
Authors: Van der Burght M , Schønemann N K , Laursen J K , Arendt-Nielsen L , Bjerring P
Published: July 2023

The topical anaesthetic formulation EMLA cream (eutectic mixture of local anaesthetics) is increasingly used prior to minor painful procedures performed on genital mucosa. The aim of the present study was to gain knowledge about the correlation between duration of application of EMLA and duration of analgesia. Three different application times//5, 10 and 20 min//were investigated in 12 healthy young women. Pin/prick pain thresholds to argon laser stimulation were measured before and after application of EMLA. Repeated measurements were performed until normal sensitivity returned. No significant difference in duration of analgesia was seen between the three application times. However, 10 min application time tended to produce the longest mean duration of analgesia (22.2 min). Surprisingly, a large inter/individual variation in duration of analgesia was seen. Thus, after 10 min application time the shortest duration of sufficient analgesia was 5 min, while the longest was 46 min. On the basis of this work, it seems advisable to start minor surgery on genital mucosa after about 10 min application time, and it is recommended to bear in mind that the duration of analgesia varies and may be very short in some patients.