bluuebunny/crossref_metadata_2025 huggingface dataset:
Genome-Wide Identification, Characterization, and Expression Profiling of TaDUF668 Gene Family in Triticum aestivum
Authors: Yin Xiaohui , Yuan Yi , Han Xiaowen , Han Shuo , Li Yiting , Ma Dongfang , Fang Zhengwu , Gong Shuangjun , Yin Junliang
Published: August 2023

DUF668s, a plant-specific gene family, encode proteins containing domain of unknown function (DUF) domains. Despite their essential functions, there is a lack of insight into Triticum aestivum TaDUF668s. Here, 31 TaDUF668s were identified from the wheat genome; according to phylogenetic relationships, they were named TaDUF668-01 to TaDUF668-31. All TaDUF668s were hydrophilic and unstable proteins. There were 22 TaDUF668s that showed subcellular localization in nucleus. Evolutionary analysis demonstrated that TaDUF668s had undergone strong purifying selection, and fragment duplication plays major role in TaDUF668 family expansion. Cis-element prediction displayed that over 90% of TaDUF668 promoter regions contain the growth and abiotic responsiveness element. Consistently, expression profiling showed that TaDUF668s were highly induced in five wheat growth and development stages, seven main different tissues, five abiotic stresses, and five pathogenic stresses. In total, 12 TaDUF668s were targeted by 20 miRNAs through the inhibition of translation and cleavage patterns. RT-qPCR results confirmed that the expression of six TaDUF668s was significantly regulated by NaCl, PEG, F. graminearum, and P. striiformis; nevertheless, the regulation patterns were different. In summary, through systematic identification, characterization, evolutionary analysis, and expression profiling, a comprehensive understanding of TaDUF668 has been obtained, which lays a foundation for further functional studies of TaDUF668.
Efficiency of Strawberry Cultivation under the Effect of Different Types of Plants in a Soilless System in the High-Altitude Regions of Southern Brazil
Authors: Palombini Mário C. , Palencia Pedro , Pavão Jessé Marques S. J. , Chiomento José L. T.
Published: August 2023

By analyzing the growth and development of different types of strawberry plants, the researchers were able to evaluate the interference with the horticultural potential of the cultivar ‘Albion’. The five treatments that have been studied corresponded to different types of strawberry plants: mini plants in trays with young roots, plants in trays with mature roots and an initial reduction of fertigation, plants in trays with mature roots, plants with bare roots, and plants in trays with mature roots preserved by the cold. In bags containing coconut fiber, the experiment was laid out in a randomized block design with three replications. The attributes evaluated were the total yield per plant, the total yield per plant with fruits above 10 g, the total yield per hectare, the total yield per hectare with fruits above 10 g, the average plant diameter, the average number of buds per plant, and the average number of stolons per plant. There were no significant effects among the plant types concerning yield production. Plants in cold-preserved trays with mature roots showed a higher number of buds than plants in trays with mature roots. Cold-preserved tray plants with mature roots produced the highest number of stolons, followed by bare-root plants. It is concluded that the types of strawberry seedlings did not influence the productive performance of the strawberry ‘Albion’ cultivar. However, cold-preserved tray plants with mature roots showed better vegetative performance, with a yield of a higher number of buds and stolons. However, the results were influenced by the agroclimatic conditions of the producing region; due to climatic interference in the behaviour of the plants about the factors studied, it is recommended to repeat the experiment for a better understanding of the objectives.
MtTGA1 Transcription Factor Enhances Salt Tolerance through Hormonal Regulation and Antioxidant Enzyme Activity in Medicago truncatula
Authors: Li Shuwen , Xie Hong , Chen Yinglong , Jia Wei , Dong Di , Wang Mengdi , Li Yinruizhi , Zhang Tiejun , Han Liebao , Chao Yuehui
Published: August 2023

The TGACG motif-binding factor1 (TGA1) transcription factor, in which belongs to the bZIP transcription factor family and has vast application potential in plant growth and development. Here, we cloned the gene of the MtTGA1 transcription factor from Medicago truncatula. The MtTGA1 promoter region contains a diverse range of photoregulatory and hormonal regulatory elements. The expression profile of MtTGA1 indicated its highest expression in the root. Additionally, the expression level of MtTGA1 was significantly upregulated after SA and BR treatments and showed a downward trend after GA and ABA treatments. To explore the potential function of MtTGA1, we treated the transgenic plants with salt treatment for 15 days, and the results showed that transgenic plants demonstrated significantly longer root lengths and heightened activities of antioxidant enzymes such as ascorbic acid catalase (APX), peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD) in their roots and leaves. The levels of endogenous hormones, including ABA and BR were improved in transgenic plants, with a marked change in the morphology of their leaf cells. Transcriptome analysis identified a total of 193 differentially expressed genes, which were significantly enriched in the pathways of “Brassinosteroid biosynthesis”, “Ascorbate and aldarate metabolism”, and “Plant hormone signal transduction”. Furthermore, MtTGA1 was found to interact with the SPX domain-containing protein 1 (SPX1) in Medicago truncatula. In conclusion, these results are beneficial for further studies about the plant growth and development regulatory network mediated by the TGA1 transcription factor family.
Soil Dynamics and Crop Yield Modeling Using the MONICA Crop Simulation Model and Time Series Forecasting Methods
Authors: Mirpulatov Islombek , Gasanov Mikhail , Matveev Sergey
Published: August 2023

Crop simulation models are an important tool for assessing agroecosystem performance and the impact of agrotechnologies on soil cover condition. However, the high uncertainty and labor intensiveness of long-term weather forecasting limits the applicability of such models. A possible solution may be to use time series forecasting models (SARIMAX and Prophet) and artificial neural-network-based technologies (Neural Prophet). This work compares the applicability of these methods for modeling soil condition dynamics and agroecosystem performance using the MONICA simulation model for Voronic Chernozems in the Kursk region of Russia. The goal is to determine which weather indicators are most important for the yield forecast and to choose the most appropriate methods for forecasting weather scenarios for agricultural modeling. Crop rotation of soybean and sugar beet was simulated, with agricultural techniques and fertilizer usage considered as factors. We demonstrated the high sensitivity of aboveground biomass production and soil moisture dynamics to daily temperature fluctuations and precipitation during the vegetation period. The dynamics of the leaf area index and nitrate content showed less sensitivity to the daily fluctuations of temperature and precipitation. Among the proposed forecasting methods, both SARIMAX and the Neural Prophet algorithm demonstrated the ability to forecast weather to model the dynamics of crop and soil conditions with the highest degree of approximation to actual observations. For the dynamic of the crop yield of soybean, the SARIMAX model exhibited the most favorable coefficient of determination, R2, while for sugar beet, the Neural Prophet model achieved superior R2 levels of 0.99 and 0.98, respectively.
Understanding Age-Related Longitudinal Dynamics in Abundance and Diversity of Dominant Culturable Gut Lactic Acid Bacteria in Pastured Goats
Authors: ElHadedy Doaa E. , Kim Chyer , Yousuf Adnan B. , Wang Zhenping , Ndegwa Eunice N.
Published: August 2023

Understanding gut lactic acid bacteria (LAB) in healthy hosts is an important first step in selecting potential probiotic species. To understand the dynamics of LAB in healthy goats, a cohort of thirty-seven healthy new-born goat kids was studied over a ten-month period. Total LAB was quantified using SYBR green qPCR. Seven hundred LAB isolates were characterized using microscopy, M13 RAPD genotyping and 16S rDNA sequencing. The highest and lowest LAB counts were detected at one week and ten months of age, respectively. Diverse LAB species were detected, whose identity and prevalence varied with age. The main isolates belonged to Limosilactobacillus reuteri, Limosilactibacillus fermentum, Lactobacillus johnsonni, Ligilactobacillus murinus, Ligilactobacillus salivarius, Limosilactobacillus mucosae, Lactiplantibacillus plantarum, Ligilactobacillus agilis, Lactobacillus acidophilus/amyolovolus, Pediococcus spp. and Enterococcus spp. Uniquely, L. reuteri and Pediococcus spp. were most common in pre- and peri-weaned goats, while Lactobacillus mucosae and Enterococcus spp. were predominant in goats one month and older. Based on RAPD genotyping, L. reuteri had the highest genotypic diversity, with age being a factor on the genotypes detected. This data may be relevant in the selection of age-specific probiotics for goats. The findings may also have broader implications by highlighting age as a factor for consideration in probiotic bacteria selection in other animal hosts.
Sensing of Antibiotic–Bacteria Interactions
Authors: Baranova Anna A. , Tyurin Anton P. , Korshun Vladimir A. , Alferova Vera A.
Published: August 2023

Sensing of antibiotic–bacteria interactions is an important area of research that has gained significant attention in recent years. Antibiotic resistance is a major public health concern, and it is essential to develop new strategies for detecting and monitoring bacterial responses to antibiotics in order to maintain effective antibiotic development and antibacterial treatment. This review summarizes recent advances in sensing strategies for antibiotic–bacteria interactions, which are divided into two main parts: studies on the mechanism of action for sensitive bacteria and interrogation of the defense mechanisms for resistant ones. In conclusion, this review provides an overview of the present research landscape concerning antibiotic–bacteria interactions, emphasizing the potential for method adaptation and the integration of machine learning techniques in data analysis, which could potentially lead to a transformative impact on mechanistic studies within the field.
Molecular Cloning, Expression Analyses, and Physiological Roles of Cathelicidins in the Bursa of Fabricius of the Japanese Quail, Coturnix japonica
Authors: Ikeda Takumi , Kondo Hirotada , Nunomura Daiki , Sato Genki , Ito Machi , Yamanaka Nanako , Iwamuro Shawichi , Hasunuma Itaru , Kikuyama Sakae , Kobayashi Tetsuya
Published: August 2023

Antimicrobial peptides (AMPs) act directly on pathogens and maintain the anti-inflammatory effects and activation of immunocompetent cells. Therefore, the activation of the immune system in poultry via the elevation of endogenous AMPs has been attempted. In this study, we focused on the host defense mechanisms in the bursa of Fabricius (BF) of Japanese quail, cloned the cDNA of cathelicidin (CATH)-1 to -3, and analyzed their expression sites. In situ hybridization experiments revealed the mRNA expression of the CATHs in the interfollicular epithelium surrounding the lumen of the quail BF, which suggests that each CATH may exert its antimicrobial action directly in the BF. The intravenous injection of bacterial lipoteichoic acid and lipopolysaccharide endotoxins into the quail promoted the mRNA expression of CATH-1 and CATH-3 in the BF. The addition of CATH-1 or CATH-2 at the time of the antigen injection into mice resulted in antiserum with high antibody titers. Ad libitum administration of butyrate, a short-chain fatty acid, in the drinking water induced an increase in CATH-2 mRNA expression in the BF under certain conditions. These results may improve the defense mechanisms of quail by stimulating CATH expression in the BF through their diet.
Prevalence and Antimicrobial Resistance Profile of Diarrheagenic Escherichia coli from Fomites in Rural Households in South Africa
Authors: Rakhalaru Phathutshedzo , Munzhedzi Lutendo , Abia Akebe Luther King , Kabue Jean Pierre , Potgieter Natasha , Traore Afsatou Ndama
Published: August 2023

Diarrheagenic Escherichia coli (DEC) pathotypes are the leading cause of mortality and morbidity in South Asia and sub-Saharan Africa. Daily interaction between people contributes to the spreading of Escherichia coli (E. coli), and fomites are a common source of community-acquired bacterial infections. The spread of bacterial infectious diseases from inanimate objects to the surrounding environment and humans is a serious problem for public health, safety, and development. This study aimed to determine the prevalence and antibiotic resistance of diarrheagenic E. coli found in toilets and kitchen cloths in the Vhembe district, South Africa. One hundred and five samples were cultured to isolate E. coli: thirty-five samples were kitchen cloths and seventy-five samples were toilet swabs. Biochemical tests, API20E, and the VITEK®-2 automated system were used to identify E. coli. Pathotypes of E. coli were characterised using Multiplex Polymerase Chain Reaction (mPCR). Nine amplified gene fragments were sequenced using partial sequencing. A total of eight antibiotics were used for the antibiotic susceptibility testing of E. coli isolates using the Kirby–Bauer disc diffusion method. Among the collected samples, 47% were positive for E. coli. DEC prevalence was high (81%), with ETEC (51%) harboring lt and st genes being the most dominant pathotype found on both kitchen cloths and toilet surfaces. Diarrheagenic E. coli pathotypes were more prevalent in the kitchen cloths (79.6%) compared with the toilet surfaces. Notably, hybrid pathotypes were detected in 44.2% of the isolates, showcasing the co-existence of multiple pathotypes within a single E. coli strain. The antibiotic resistance testing of E. coli isolates from kitchen cloths and toilets showed high resistance to ampicillin (100%) and amoxicillin (100%). Only E. coli isolates with hybrid pathotypes were found to be resistant to more than three antibiotics. This study emphasizes the significance of fomites as potential sources of bacterial contamination in rural settings. The results highlight the importance of implementing proactive measures to improve hygiene practices and antibiotic stewardship in these communities. These measures are essential for reducing the impact of DEC infections and antibiotic resistance, ultimately safeguarding public health.
Technologically Driven Approaches for the Integrative Use of Wild Blackthorn (Prunus spinosa L.) Fruits in Foods and Nutraceuticals
Authors: Nistor Oana Viorela , Milea Ștefania Adelina , Păcularu-Burada Bogdan , Andronoiu Doina Georgeta , Râpeanu Gabriela , Stănciuc Nicoleta
Published: August 2023

Different technological approaches were used in this study for the valorization of blackthorn (Prunus spinosa L.) fruits in marmalade, jam, jelly, and nutraceuticals. Marmalade showed the highest concentrations of polyphenols (7.61 ± 0.05 mg gallic acid equivalents/g dry weight (DW)) and flavonoids (4.93 ± 0.22 mg catechin equivalents/g DW), whereas jam retained the highest content of anthocyanins (66.87 ± 1.18 mg cyanidin-3-O-glucoside equivalents/g DW). A good correlation between polyphenol and flavonoid contents and antioxidant activity was found, the highest value being 21.29 ± 1.36 mmol Trolox/g DW for marmalade. Alternatively, the fresh pulp was enriched with inulin, followed by inoculation with Lactobacillus acidophilus, and freeze-dried, allowing a powder to be obtained with a viable cell content of 6.27 × 107 CFU/g DW. A chromatographic analysis of blackthorn skin revealed that myricetin (2.04 ± 0.04 mg/g DW) was the main flavonoid, followed by (+)–catechin (1.80 ± 0.08 mg/g DW), (−)-epicatechin (0.96 ± 0.02 mg/g DW), and vanillic acid (0.94 ± 0.09 mg/g DW). The representative anthocyanins were cyanidin 3-O-glucoside, cyanidin 3-O-rutinoside, and peonidin 3-O-glucoside, with an average concentration of 0.75 mg/g DW. The skin extract showed comparable IC50 values for tyrosinase (1.72 ± 0.12 mg/mL), α-amylase (1.17 ± 0.13 mg/mL), and α-glucosidase (1.25 ± 0.26 mg/mL). The possible use of kernels as calorific agents was demonstrated through the evaluation of calorific power of 4.9 kWh/kg.
Exploring the Relationship between Antioxidant Enzymes, Oxidative Stress Markers, and Clinical Profile in Relapsing–Remitting Multiple Sclerosis
Authors: Bizoń Anna , Chojdak-Łukasiewicz Justyna , Budrewicz Sławomir , Pokryszko-Dragan Anna , Piwowar Agnieszka
Published: August 2023

We aimed to investigate the extent of alterations in the pro/antioxidant balance in the blood of patients with relapsing–remitting multiple sclerosis (RRMS) in relation to drug-modified therapy, gender, disability score, and disease duration. 161 patients (67 men and 94 women, aged 24–69 years, median 43.0) and 29 healthy individuals (9 men and 20 women, aged 25–68 years, median 41.0) were included in the study. We measured the activity of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) as well as the concentration of interleukin-6 (IL-6), lipid peroxidation parameters (LPO), total oxidant status (TOS), and total antioxidant capacity (TAS). The activity of SOD did not show any significant differences between patients with RRMS and the control group in our study. In contrast, significant decreased GPx activity and increased CAT activity was observed in the blood of patients with RRMS compared to the control group. Additionally, the activity of CAT was influenced by gender and the use of disease-modifying therapies. Disease-modifying therapies also affected the concentration of TOS, TAS, and LPO. Our studies indicated that enhancing GPx activity may be more beneficial to providing potential therapeutic strategies aimed at modulating antioxidant defenses to mitigate oxidative stress in this disease.
Insight into Physiological and Biochemical Determinants of Salt Stress Tolerance in Tetraploid Citrus
Authors: Bonnin Marie , Favreau Bénédicte , Soriano Alexandre , Leonhardt Nathalie , Oustric Julie , Lourkisti Radia , Ollitrault Patrick , Morillon Raphaël , Berti Liliane , Santini Jérémie
Published: August 2023

Citrus are classified as salt-sensitive crops. However, a large diversity has been observed regarding the trends of tolerance among citrus. In the present article, physiological and biochemical studies of salt stress tolerance were carried out according to the level of polyploidy of different citrus genotypes. We particularly investigated the impact of tetraploidy in trifoliate orange (Poncirus trifoliata (L.) Raf.) (PO4x) and Cleopatra mandarin (Citrus reshni Hort. Ex Tan.) (CL4x) on the tolerance to salt stress compared to their respective diploids (PO2x and CL2x). Physiological parameters such as gas exchange, ions contents in leaves and roots were analyzed. Roots and leaves samples were collected to measure polyphenol, malondialdehyde (MDA), ascorbate and H2O2 contents but also to measure the activities of enzymes involved in the detoxification of active oxygen species (ROS). Under control conditions, the interaction between genotype and ploidy allowed to discriminate different behavior in terms of photosynthetic and antioxidant capacities. These results were significantly altered when salt stress was applied when salt stress was applied. Contrary to the most sensitive genotype, that is to say the diploid trifoliate orange PO2x, PO4x was able to maintain photosynthetic activity under salt stress and had better antioxidant capacities. The same observation was made regarding the CL4x genotype known to be more tolerant to salt stress. Our results showed that tetraploidy may be a factor that could enhance salt stress tolerance in citrus.
A Critical Review of Kaempferol in Intestinal Health and Diseases
Authors: Chen Jun , Zhong Haopeng , Huang Zhouyin , Chen Xingping , You Jinming , Zou Tiande
Published: August 2023

Kaempferol, a secondary metabolite found in plants, is a naturally occurring flavonoid displaying significant potential in various biological activities. The chemical structure of kaempferol is distinguished by the presence of phenyl rings and four hydroxyl substituents, which make it an exceptional radical scavenger. Most recently, an increasing number of studies have demonstrated the significance of kaempferol in the regulation of intestinal function and the mitigation of intestinal inflammation. The focus of the review will primarily be on its impact in terms of antioxidant properties, inflammation, maintenance of intestinal barrier function, and its potential in the treatment of colorectal cancer and obesity. Future research endeavors should additionally give priority to investigating the specific dosage and duration of kaempferol administration for different pathological conditions, while simultaneously conducting deeper investigations into the comprehensible mechanisms of action related to the regulation of aryl hydrocarbon receptor (AhR). This review intends to present novel evidence supporting the utilization of kaempferol in the regulation of gut health and the management of associated diseases.
Multi-Omics Approach Reveals Prebiotic and Potential Antioxidant Effects of Essential Oils from the Mediterranean Diet on Cardiometabolic Disorder Using Humanized Gnotobiotic Mice
Authors: Sánchez-Quintero María José , Delgado Josué , Martín Chaves Laura , Medina-Vera Dina , Murri Mora , Becerra-Muñoz Víctor M. , Estévez Mario , Crespo-Leiro María G. , Paz López Guillermo , González-Jiménez Andrés , A. G. Ranea Juan , Queipo-Ortuño María Isabel , Plaza-Andrades Isaac , Rodríguez-Capitán Jorge , Pavón-Morón Francisco Javier , Jiménez-Navarro Manuel F.
Published: August 2023

Essential oils sourced from herbs commonly used in the Mediterranean diet have demonstrated advantageous attributes as nutraceuticals and prebiotics within a model of severe cardiometabolic disorder. The primary objective of this study was to assess the influences exerted by essential oils derived from thyme (Thymus vulgaris) and oregano (Origanum vulgare) via a comprehensive multi-omics approach within a gnotobiotic murine model featuring colonic microbiota acquired from patients diagnosed with coronary artery disease (CAD) and type-2 diabetes mellitus (T2DM). Our findings demonstrated prebiotic and potential antioxidant effects elicited by these essential oils. We observed a substantial increase in the relative abundance of the Lactobacillus genus in the gut microbiota, accompanied by higher levels of short-chain fatty acids and a reduction in trimethylamine N-oxide levels and protein oxidation in the plasma. Moreover, functional enrichment analysis of the cardiac tissue proteome unveiled an over-representation of pathways related to mitochondrial function, oxidative stress, and cardiac contraction. These findings provide compelling evidence of the prebiotic and antioxidant actions of thyme- and oregano-derived essential oils, which extend to cardiac function. These results encourage further investigation into the promising utility of essential oils derived from herbs commonly used in the Mediterranean diet as potential nutraceutical interventions for mitigating chronic diseases linked to CAD and T2DM.
Antioxidant Properties of Bioactive Compounds in Fruit and Vegetable Waste
Authors: Jiménez-Moreno Nerea , Esparza Irene , Ancín-Azpilicueta Carmen
Published: August 2023

In recent years, great interest has arisen in the study of compounds with antioxidant activity present in agri-food residues [...]
Oxidative Stress and Its Regulation in Diabetic Retinopathy
Authors: Haydinger Cameron D. , Oliver Genevieve F. , Ashander Liam M. , Smith Justine R.
Published: August 2023

Diabetic retinopathy is the retinal disease associated with hyperglycemia in patients who suffer from type 1 or type 2 diabetes. It includes maculopathy, involving the central retina and characterized by ischemia and/or edema, and peripheral retinopathy that progresses to a proliferative stage with neovascularization. Approximately 10% of the global population is estimated to suffer from diabetes, and around one in 5 of these individuals have diabetic retinopathy. One of the major effects of hyperglycemia is oxidative stress, the pathological state in which elevated production of reactive oxygen species damages tissues, cells, and macromolecules. The retina is relatively prone to oxidative stress due to its high metabolic activity. This review provides a summary of the role of oxidative stress in diabetic retinopathy, including a description of the retinal cell players and the molecular mechanisms. It discusses pathological processes, including the formation and effects of advanced glycation end-products, the impact of metabolic memory, and involvements of non-coding RNA. The opportunities for the therapeutic blockade of oxidative stress in diabetic retinopathy are also considered.
Effect of Purple Sweet Potato Using Different Cooking Methods on Cytoprotection against Ethanol-Induced Oxidative Damage through Nrf2 Activation in HepG2 Cells
Authors: Kim Dagyeong , Kim Yoonjeong , Kim Younghwa
Published: August 2023

The aim of this study was to investigate the effects of different cooking methods on the hepatoprotective effects of purple sweet potatoes against alcohol-induced damage in HepG2 cells. Purple sweet potatoes (Ipomeoea batatas L. Danjami) were subjected to different cooking methods, including steaming, roasting, and microwaving. Steaming resulted in a higher cytoprotective effect against alcohol damage than the other cooking methods. Additionally, the highest inhibition of glutathione depletion and production of reactive oxygen species against alcohol-induced stress were observed in raw and/or steamed purple sweet potatoes. Compared to roasted and/or microwaved samples, steamed samples significantly increased the expression of NADPH quinone oxidoreductase 1, heme oxygenase 1, and gamma glutamate-cysteine ligase in alcohol-stimulated HepG2 cells via the activation of nuclear factor erythroid 2-related factor 2. Moreover, ten anthocyanins were detected in the raw samples, whereas five, two, and two anthocyanins were found in the steamed, roasted, and microwaved samples, respectively. Taken together, steaming purple sweet potatoes could be an effective cooking method to protect hepatocytes against alcohol consumption. These results provide useful information for improving the bioactive properties of purple sweet potatoes using different cooking methods.
Cytotoxic Effect of L-Methioninase from Brevibacterium linens BL2 in Combination with Etoposide against Glioblastoma Cells
Authors: İpek Semih Latif , Özdemir Meryem Damla , Göktürk Dilek
Published: August 2023

L-methioninase degrades methionine, which is essential in methionine-dependent cancer cells, resulting in specific cell death. Normal cells can synthesize their own methionine amino acids even in the absence of exogenous methionine. This selective targeting of cancer cells makes L-methioninase a promising therapeutic candidate for cancer. In this study, L-methioninase was partially purified from Brevibacterium linens BL2. The specific activity of the enzyme was found as 3.055 units/mg. IC50 values (24 h) of the enzyme were 5.792 units/mL for U87MG cell line and 5.215 units/mL for T98G cell line. When L-methioninase and etoposide were used in combination, synergistic cytotoxic and cell migration inhibition effects on U87MG and T98G cells alongside decreased cytotoxic activity on the Mouse Embryonic Fibroblast and HaCaT cells compared to etoposide alone were observed. Additionally, colony numbers of U87MG cells were significantly reduced by L-methioninase and etoposide administration after 21 days of incubation. Furthermore, L-methioninase suppressed the expression levels of survivin and c-Myc while increasing the expression level of Caspase-3 in both glioblastoma cell lines. These effects were enhanced when etoposide was used in combination with etoposide. This investigation reveals that the L-methioninase enzyme not only exhibited cytotoxic effects on U87MG and T98G cells but also enhanced the anti-proliferative effects of etoposide when used in combination while also demonstrating fewer adverse effects on normal cells.
Design and Implementation of a Low-Cost Torque Sensor for Manipulators
Authors: Pástor Miroslav , Hagara Martin , Gašpár Štefan , Sapieta Milan
Published: August 2023

Humanoid and collaborative robots find their application in numerous sectors, such as automotive, electrical and mechanical engineering, not excluding the field of bioengineering. They replace repetitive and often monotonous human activity. As the trend nowadays is for continuous optimization of production processes, their advantage is easy relocation and operative application to new tasks, which allows the automation of practically all manual work. A common feature of manipulators is the control of the positioning of the actuators, primarily by adjusting the parameters of the drive units. Feedback is often implemented through various sensors that provide real-time information. However, most of the sensors in use do not provide information that would allow obtaining data on the history of the operating load in order to assess the further safe and reliable operation of the mechanical parts. This paper presents a low-cost torque sensor that was proposed by modifying the design of an existing part. The torque sensor was developed on the principle of strain gauge measurement. The results of strain–stress analysis obtained by numerical modelling were experimentally validated under static and dynamic loading. Practical application is mainly the development and long-term testing of prototypes of various types of manipulators and collaborative robots, where high accuracy and repeatability of positioning are essential.
Analysis of Vibration Characteristics of Planetary Gearbox with Broken Sun Gear Based on Phenomenological Model
Authors: Zou Mengting , Ma Jun , Xiong Xin , Li Rong
Published: August 2023

To investigate the vibration properties in healthy and fault conditions of planetary gearboxes, a phenomenological model is constructed to present the vibration spectrum structure. First, the effects of the base deflection of the gear fillet and the flexibility between the root circle and the base circle on the time-varying meshing stiffness are considered in order to construct an equivalent model of time-varying mesh stiffness and broken tooth faults, exploring the law of variation for meshing stiffness when differently sized faults occur on the sun gear. Then, considering both the effect of the vibration transfer path and the meshing impacts, we establish phenomenological models of planetary gears under healthy and fault conditions. By comparing and analyzing the phenomenological model based on the cosine function to verify the effectiveness of the proposed model. The experimental results show that the error of the proposed model is 1.38% lower than that of the traditional phenomenological model, and the proposed model can accurately analyze the frequency, amplitude, and sideband characteristics of the vibration signals of sun gear with different degrees of broken tooth, which can be used for the local fault diagnosis of planetary gearboxes.
Value Assessment of UGC Short Videos through Element Mining and Data Analysis
Authors: Fang Jinyu , Ni Yuan , Zhang Jian
Published: August 2023

UGC short videos play a crucial role in sharing information and disseminating content in the era of new information technology. Accurately assessing the value of UGC short videos is highly significant for the sustainable development of self-media platforms and the secure governance of cyberspace. This study proposes a method for assessing the value of UGC short videos from the perspective of element mining and data analysis. The method involves three steps. Firstly, the text clustering algorithm and topic mapping visualization technology are utilized to identify elements for assessing the value of UGC short videos and construct an assessment index system. Secondly, structured data indexes are quantified using platform data statistics, while unstructured data indexes are quantified using the LSTM fine-grained sentiment analysis model. Lastly, the VIKOR model, incorporating an improved gray correlation coefficient, is employed to effectively evaluate the value of UGC short videos. The empirical results indicate that the value of current domestic UGC short videos is primarily associated with three dimensions: the creators, the platforms, and the users. It encompasses 11 value elements, including fan popularity, economic returns of creation, and frequency of interaction. Additionally, we assess the value of short videos within the mainstream partitions of the Bilibili platform and generate a value radar chart. Our findings reveal that short videos in game partitions generate higher revenue for creators and platforms but may neglect users’ needs for knowledge, culture, and other content. Conversely, short videos in the knowledge, food, and music partitions demonstrate specific distinctions in fulfilling users’ requirements. Ultimately, we offer personalized recommendations for the future development of high-value UGC short videos within the mainstream partitions.
Machine Learning-Based Text Classification Comparison: Turkish Language Context
Authors: Alzoubi Yehia Ibrahim , Topcu Ahmet E. , Erkaya Ahmed Enis
Published: August 2023

The growth in textual data associated with the increased usage of online services and the simplicity of having access to these data has resulted in a rise in the number of text classification research papers. Text classification has a significant influence on several domains such as news categorization, the detection of spam content, and sentiment analysis. The classification of Turkish text is the focus of this work since only a few studies have been conducted in this context. We utilize data obtained from customers’ inquiries that come to an institution to evaluate the proposed techniques. Classes are assigned to such inquiries specified in the institution’s internal procedures. The Support Vector Machine, Naïve Bayes, Long Term-Short Memory, Random Forest, and Logistic Regression algorithms were used to classify the data. The performance of the various techniques was then analyzed after and before data preparation, and the results were compared. The Long Term-Short Memory technique demonstrated superior effectiveness in terms of accuracy, achieving an 84% accuracy rate, surpassing the best accuracy record of traditional techniques, which was 78% accuracy for the Support Vector Machine technique. The techniques performed better once the number of categories in the dataset was reduced. Moreover, the findings show that data preparation and coherence between the classes’ number and the number of training sets are significant variables influencing the techniques’ performance. The findings of this study and the text classification technique utilized may be applied to data in dialects other than Turkish.
An Exploratory In Vitro Microcomputed Tomographic Investigation of the Efficacy of Semicircular Apicoectomy Performed with Trephine Bur
Authors: Nagy Eszter , Vőneki Brigitta , Vásárhelyi Lívia , Szenti Imre , Fráter Márk , Kukovecz Ákos , Antal Márk Ádám
Published: August 2023

Purpose: Recently, a novel approach to apicoectomy has emerged, involving the use of a hollow trephine with a surgical guide. This innovative technique creates a semicircular section, in contrast to the conventional straight cut (with a bur). The semicircular shape of this section raises questions about the method’s effectiveness in eliminating supernumerary canals (SNCs), which are auxiliary canals alongside the main root canal. These canals pose a risk of further infection if not thoroughly eliminated. The aim of this exploratory study was to assess the efficacy of the proposed method in the removal of SNCs. Methods: A sample of 200 extracted single-rooted human teeth was rigorously narrowed down by multiple steps to 33 specimens that contained SNCs in the apical third. These specimens underwent apical semicircular sectioning, removing the apical 3 mm of the root. The specimens then underwent repeated CT scanning. For samples with residual SNCs, the section was transformed into a straight section and another CT scan was performed. Results: The semicircular section eliminated all SNCs in 94% of the specimens, and it eliminated 97.3% of the SNCs in all specimens. The straight-line sectioning eliminated 98.6% of all SNCs and eliminated all SNCs in 96.97% of the SNC-containing teeth. Conclusions: The efficacy of semicircular apicoectomy performed with a trephine appears to be comparable (χ2 = 1.00, df = 1, and p = 0.317) to that of the conventional straight apicoectomy performed with a bur as reported in the literature.
A Novel Approach to Satellite Component Health Assessment Based on the Wasserstein Distance and Spectral Clustering
Authors: Hui Yongchao , Cheng Yuehua , Jiang Bin , Han Xiaodong , Yang Lei
Published: August 2023

This research presents a multiparameter approach to satellite component health assessment aimed at addressing the increasing demand for in-orbit satellite component health assessment. The method encompasses three key enhancements. Firstly, the utilization of the Wasserstein distance as an indicator simplifies the decision-making process for assessing the health of data distributions. This enhancement allows for a more robust handling of noisy sensor data, resulting in improved accuracy in health assessment. Secondly, the original limitation of assessing component health within the same parameter class is overcome by extending the evaluation to include multiple parameter classes. This extension leads to a more comprehensive assessment of satellite component health. Lastly, the method employs spectral clustering to determine the boundaries of different health status classes, offering an objective alternative to traditional expert-dependent approaches. By adopting this technique, the proposed method enhances the objectivity and accuracy of the health status classification. The experimental results show that the method is able to accurately describe the trends in the health status of components. Its effectiveness in real-time health assessment and monitoring of satellite components is confirmed. This research provides a valuable reference for further research on satellite component health assessment. It introduces novel and enhanced ideas and methodologies for practical applications.
A Rapid and Nondestructive Detection Method for Rapeseed Quality Using NIR Hyperspectral Imaging Spectroscopy and Chemometrics
Authors: Wang Du , Li Xue , Ma Fei , Yu Li , Zhang Wen , Jiang Jun , Zhang Liangxiao , Li Peiwu
Published: August 2023

In this study, a fast and non-destructive method was proposed to analyze rapeseed quality parameters with the help of NIR hyperspectral imaging spectroscopy and chemometrics. Hyperspectral images were acquired in the reflectance mode. Meanwhile, the region of interest was extracted from each image by the regional growth algorithm. The kernel partial least square regression was used to build prediction models for crude protein content, oil content, erucic acid content, and glucosinolate content of rapeseed. The results showed that the correlation coefficients were 0.9461, 0.9503, 0.9572, and 0.9335, whereas the root mean square errors of prediction were 0.5514%, 0.5680%, 2.8113%, and 10.3209 µmol/g for crude protein content, oil content, erucic acid content, and glucosinolate content, respectively. It demonstrated that NIR hyperspectral imaging is a promising tool to determine rapeseed quality parameters in a rapid and non-invasive manner.
Implementation of a Nutrition-Oriented Clinical Decision Support System (CDSS) for Weight Loss during the COVID-19 Epidemic in a Hospital Outpatient Clinic: A 3-Month Controlled Intervention Study
Authors: Detopoulou Paraskevi , Papandreou Panos , Papadopoulou Lida , Skouroliakou Maria
Published: August 2023

Clinical Decision Support Systems (CDSSs) facilitate evidence-based clinical decision making for health professionals. Few studies have applied such systems enabling distance monitoring in the COVID-19 epidemic, especially in a hospital setting. The purpose of the present work was to assess the clinical efficacy of CDSS-assisted dietary services at a general hospital for patients intending to lose weight during the COVID-19 pandemic. Thirty-nine patients (28 men, 71.8%) comprised the intervention group and 21 patients (four men, 16%) of the control group. After a 3-month CDSS intervention, reductions in both body weight (mean ± standard deviation (SD): 95.5 ± 21.8 vs. 90.6 ± 19.9 kg, p < 0.001) and body mass index (BMI) (median, interquartile range (IQR): 35.2, 28.4–37.5 vs. 33.2, 27.4–35.4 kg/m2, p < 0.001) were observed. Beneficial effects were also recorded for total body fat (44.9 ± 11.3 vs. 41.9 ± 10.5%, p < 0.001), glycated hemoglobin (5.26 ± 0.55 vs. 4.97 ± 0.41%, p = 0.017) (mean ± SD) and triglycerides (137, 115–152 vs. 130, 108–160 mg/dL, p = 0.005) (medians, IQR). Lean tissue was borderline decreased (25.4, 21.7–29.1 vs. 24.6, 21.8–27.9 kg, p = 0.050). No changes were documented in the control group. In multivariate linear regression models, serum triglycerides were inversely associated with % absolute weight loss (B = −0.018, standard error (SE) = 0.009, p = 0.050) in the CDSS intervention group. In women, a principal component analysis-derived pattern characterized by high BMI/lean tissue was positively related to % absolute weight loss (B = 20.415, SE = 0.717, p = 0.028). In conclusion, a short-term CDSS-facilitated intervention beneficially affected weight loss and other cardiovascular risk factors.
Thermal-Hydraulic Modeling of Oil-Immersed Motor Pump
Authors: Shi Junqiang , Li Ziyang , Gao Jingcheng , Chen Dongjing , Li Xiaotao , Li Ying , Zhang Jin , Kong Xiangdong
Published: August 2023

The integrated design of the motor and axial piston pump eliminates the coupling structure, resulting in a compact and lightweight motor-pump structure. The challenge of motors overheating has always been a major concern. To address this issue, the hydraulic oil throughout the motor pump is utilized for cooling the high-speed motor, effectively improving the power density and heat dissipation capability of the hydraulic power unit. This integrated design approach has successfully resolved the significant issue of overheating motors, leading to enhanced performance of the hydraulic power unit. To address this concern, the entire motor pump’s oil is utilized to cool the high-speed motor. Consequently, the thermodynamic prediction of high-speed motor pumps has become increasingly important. In this study, the impact of motor heat generation on hydrodynamics is analyzed, and the heat transfer of the motor pump is investigated using the control volume method. Furthermore, thermodynamic models of hysteresis loss, eddy current loss, alternating current loss, churning loss, and throttling loss are established for the oil-immersed motor pump. The change in oil viscosity is also considered. The instantaneous temperature change rule of the oil within the oil-immersed motor pump is derived. Additionally, the influence of various working conditions such as pressure and speed on the temperature of the motor pump’s key node is examined. The experimental results indicate the accuracy of the thermodynamic calculation, and the significant effect of motor loss on the leakage temperature.
Spontaneous and Controlled Fermentation Tests in Industrial Table Olives Production
Authors: Simone Nicola , Di Loreto Giuseppina , Bacceli Martina , Di Marco Sara , Cellini Martina , Vecchiotti Giulia , Lanza Barbara
Published: August 2023

The process of debittering table olives, known as “natural”, represents the most studied method for its peculiarities. Searching among other studies, very little evidence was found about “outside the lab” tests; this encouraged our research, given the numerous external factors that could influence the whole process. In this study, we followed batch fermentation processes inside an industrial facility, testing both spontaneous and guided processes. The starter selected was a 1:1 ratio of Lactiplantibacillus plantarum B1 strain and Saccharomyces cerevisiae SC24 strain. (108 CFU/mL). Chemical-physical, microbiological, and phenolic parameters of five different barrels were analysed, with a periodic sampling (seven samplings). Results obtained confirmed logical correlations (p < 0.05 Pearson) between chemical-physical parameters (ashes vs. pulp/seed ratio, ashes vs. humidity, salt content vs. pulp/seed ratio, oil content vs. humidity) and showed a good discrimination between samples, especially regarding the phenolic profile of the different barrels (PCA and AHC). Microbiological analysis showed the evolution of microorganisms in the barrels, but the results have an unclear interpretation. No Enterobacter were found, so the safety of both process and product can be confirmed. These results highlight some criticalities of the industrial processes and represent useful data for technicians and production structures in order to improve the quality of the product and minimize losses.
Influence of Sports on Cortical Connectivity in Patients with Spinal Cord Injury-A High-Density EEG Study
Authors: Frey Vanessa N. , Renz Nora , Thomschewski Aljoscha , Langthaler Patrick B. , van Schalkwijk Frank J. , Trinka Eugen , Höller Yvonne
Published: August 2023

Background: Minutes after an injury to the spinal cord, structural and functional reorganization of the connected brain areas may be initiated. Exercise enhances this neuroplasticity in the further course of the condition, which might modulate the connectivity patterns in brain regions responsible for movement execution and imagination. However, connectivity patterns have not been analyzed as a correlate for activity effects on neuroplasticity after spinal cord injury (SCI). We hypothesize that wheelchair sport has a modulating effect on the cortical connectivity in patients with SCI, such that distinguished activity patterns can be observed between sportive and non-sportive individuals with SCI and healthy participants. Methods: Sportive (n = 16) and non-sportive (n = 7) patients with SCI as well as sportive (n = 16) and non-sportive (n = 14) healthy participants were instructed to either observe, imagine, or conduct an observed movement while high-density EEG (HD-EEG) was recorded. Functional connectivity was computed from the recorded signals, and the coefficients were compared between groups and conditions using a non-parametric repeated measures analysis. Results: We found that depending on being sportive or not, patients with SCI and controls would react differently to the conditions, but the effects depended on the location in the brain as well as the analyzed frequency range (p < 0.05). Further analysis indicates that non-sportive patients showed higher connectivity received by the right posterior parietal cortex and a lower connectivity received by the left M1 compared to sportive patients. These effects were mainly observed during movement imagination, not during movement. Sportive and non-sportive participants in the healthy control group showed smaller differences than the patients. Conclusions: The results suggest a modulative effect of sports on connectivity patterns during movement imagination and to some extent during movement. This effect was predominantly found in patients with SCI, and to a lesser extent in healthy participants with opposing connectivity patterns. We suggest that this might be due to increased cortical excitability and the elevated brain derived neurotrophic factor (BDNF) level in patients with SCI that is enhanced by exercise.
Fog Decision Support Systems: A Review of the Current Perspectives
Authors: Bari Driss , Bergot Thierry , Tardif Robert
Published: August 2023

Accurate and timely fog forecasts are needed to support decision making for various activities which are critically affected by low visibility conditions [...]