bluuebunny/crossref_metadata_2025 huggingface dataset:
A Raisin Foreign Object Target Detection Method Based on Improved YOLOv8
Authors: Ning Meng , Ma Hongrui , Wang Yuqian , Cai Liyang , Chen Yiliang
Published: August 2024

During the drying and processing of raisins, the presence of foreign matter such as fruit stems, branches, stones, and plastics is a common issue. To address this, we propose an enhanced real-time detection approach leveraging an improved YOLOv8 model. This novel method integrates the multi-head self-attention mechanism (MHSA) from BoTNet into YOLOv8’s backbone. In the model’s neck layer, selected C2f modules have been strategically replaced with RFAConv modules. The model also adopts an EIoU loss function in place of the original CIoU. Our experiments reveal that the refined YOLOv8 boasts a precision of 94.5%, a recall rate of 89.9%, and an F1-score of 0.921, with a mAP reaching 96.2% at the 0.5 IoU threshold and 81.5% across the 0.5–0.95 IoU range. For this model, comprising 13,177,692 parameters, the average time required for detecting each image on a GPU is 7.8 milliseconds. In contrast to several prevalent models of today, our enhanced model excels in mAP0.5 and demonstrates superiority in F1-score, parameter economy, computational efficiency, and speed. This study conclusively validates the capability of our improved YOLOv8 model to execute real-time foreign object detection on raisin production lines with high efficacy.
Occurrence and Favorable Enrichment Environment of Lithium in Gaoping Coal Measures: Evidence from Mineralogy and Geochemistry
Authors: Han Peiliang , Zhao Fenghua , Liu Dongna , Zhang Qi , Zhang Qinqin , Ullah Shaheed
Published: August 2024

The Carboniferous-Permian coal measure strata in the Qinshui Basin exhibit highly lithium (Li) enrichment, with substantial exploitation potential. To further explore the enrichment mechanism of lithium in coal measure strata, the No. 15 coal of the Taiyuan Formation from the Gaoping mine is taken as the research object, and its mineralogical and geochemistry characteristics are evaluated using optical microscopy, X-ray diffraction, scanning electron microscopy, inductively coupled plasma mass spectrometry, X-ray fluorescence, and infrared spectral. The results show that the No. 15 coal is semi-anthracite coal with low moisture, low ash, low volatility, and high sulfur. Organic macerals are primarily vitrinite, followed by inertinite, and liptinite is rare; the inorganic macerals (ash) are dominated by clay minerals (predominantly kaolinite, cookeite, illite, and NH4-illite), calcite, pyrite, quartz, siderite, gypsum, and zircon. The average Li content in the coal is 66.59 μg/g, with higher content in the coal parting (566.00 μg/g) and floor (396.00 μg/g). Lithium in coal occurs primarily in kaolinite, illite, cookeite, and is closely related to titanium-bearing minerals. In addition, Li in organic maceral may occur in liptinite. The No. 15 coal was formed in the coastal depositional system, and the deposition palaeoenvironment is primarily a wet–shallow water covered environment in open swamp facies; the plant tissue preservation index is poor, and aquatic or herbaceous plants dominate the plant type. The reducing environment with more terrestrial detritus, an arid climate, and strong hydrodynamic effects is favorable for Li enrichment in coal. The results have important theoretical significance for exploring the enrichment and metallogenic mechanisms of Li in coal.
Thermodynamic Analysis of Marine Diesel Engine Exhaust Heat-Driven Organic and Inorganic Rankine Cycle Onboard Ships
Authors: Ezgi Cuneyt , Kepekci Haydar
Published: August 2024

Due to increasing emissions and global warming, in parallel with the increasing world population and energy needs, IMO has introduced severe rules for ships. Energy efficiency on ships can be achieved using the organic and inorganic Rankine cycle (RC) driven by exhaust heat from marine diesel engines. In this study, toluene, R600, isopentane, and n-hexane as dry fluids; R717 and R718 as wet fluids; and R123, R142b, R600a, R245fa, and R141b as isentropic fluids are selected as the working fluid because they are commonly used refrigerants, with favorable thermal properties, zero ODP, low GWP and are good contenders for this application. The cycle and exergy efficiencies, net power, and irreversibility of marine diesel engine exhaust-driven simple RC and RC with a recuperator are calculated. For dry fluids, the most efficient fluid at low turbine inlet temperatures is n-hexane at 39.75%, while at high turbine inlet temperatures, it is toluene at 41.20%. For isentropic fluids, the most efficient fluid at low turbine inlet temperatures is R123 with 23%, while at high turbine inlet temperatures it is R141b with 23%. As an inorganic fluid, R718 is one of the most suitable working fluids at high turbine inlet temperatures of 300 °C onboard ships with a safety group classification of A1, ODP of 0, and GWP100 of 0, with a cycle efficiency of 33%. This study contributes to significant improvements in fuel efficiency and reductions in greenhouse gas emissions, leading to more sustainable and cost-effective maritime operations.
‘A World of Knowledge’: Rock Art, Ritual, and Indigenous Belief at Serranía De La Lindosa in the Colombian Amazon
Authors: Hampson Jamie , Iriarte José , Aceituno Francisco Javier
Published: August 2024

There are tens of thousands of painted rock art motifs in the Serranía de la Lindosa in the Colombian Amazon, including humans, animals, therianthropes, geometrics, and flora. For most of the last 100 years, inaccessibility and political unrest has limited research activities in the region. In this paper, we discuss findings from six years of field research and consider the role of rock art as a manifestation of Indigenous ontologies. By employing intertwining strands of evidence—a range of ethnographic sources, local Indigenous testimonies from 2021–2023, and the motifs themselves—we argue that the rock art here is connected to ritual specialists negotiating spiritual realms, somatic transformation, and the interdigitation of human and non-human worlds.
Investigating Different Interpolation Methods for High-Accuracy VTEC Analysis in Ionospheric Research
Authors: Doğanalp Serkan , Köz İrem
Published: August 2024

The dynamic structure of the ionosphere and its changes play an important role in comprehending the natural cycle by linking earth sciences and space sciences. Ionosphere research includes a variety of fields like meteorology, radio wave reflection from the atmosphere, atmospheric anomaly detection, the impact on GNSS (Global Navigation Satellite Systems) signals, the exploration of earthquake precursors, and the formation of the northern lights. To gain further insight into this layer and to monitor variations in the total electron content (TEC), ionospheric maps are created using a variety of data sources, including satellite sensors, GNSS data, and ionosonde data. In these maps, data deficiencies are addressed by using interpolation methods. The objective of this study was to obtain high-accuracy VTEC (Vertical Total Electron Content) information to analyze TEC anomalies as precursors to earthquakes. We propose an innovative approach: employing alternative mathematical surfaces for VTEC calculations, leading to enhanced change analytical interpretation for anomaly detections. Within the scope of the application, the second-degree polynomial method, kriging (point and block model), the radial basis multiquadric, and the thin plate spline (TPS) methods were implemented as interpolation methods. During a 49-day period, the TEC values were computed at three different IGS stations, generating 1176 hourly grids for each interpolation model. As reference data, the ionospheric maps produced by the CODE (Center for Orbit Determination in Europe) Analysis Center were used. This study’s findings showed that, based on statistical values, the TPS model offered more accurate results than other methods. Additionally, it has been observed that the peak values in TEC calculations based on polynomial surfaces are eliminated in TPSs.
Forecasting In-Flight Icing over Greece: Insights from a Low-Pressure System Case Study
Authors: Louka Petroula , Samos Ioannis , Gofa Flora
Published: August 2024

Forecasting in-flight icing conditions is crucial for aviation safety, particularly in regions with variable and complex meteorological configurations, such as Greece. Icing accretion onto the aircraft’s surfaces is influenced by the presence of supercooled water in subfreezing environments. This paper outlines a methodology of forecasting icing conditions, with the development of the Icing Potential Algorithm that takes into consideration the meteorological scenarios related to icing accretion, using state-of-the-art Numerical Weather Prediction model results, and forming a fuzzy logic tree based on different membership functions, applied for the first time over Greece. The synoptic situation of an organized low-pressure system passage, with occlusion, cold and warm fronts, over Greece that creates dynamically significant conditions for icing formation was investigated. The sensitivity of the algorithm was revealed upon the precipitation, cloud type and vertical velocity effects. It was shown that the greatest icing intensity is associated with single-layer ice and multi-layer clouds that are comprised of both ice and supercooled water, while convectivity and storm presence lead to also enhancing the icing formation. A qualitative evaluation of the results with satellite, radar and METAR observations was performed, indicating the general agreement of the method mainly with the ground-based observations.
Long-Term Wind and Air Temperature Patterns in the Southeastern Region of Iran through Model Simulation and Ground Observations
Authors: Hamzeh Nasim Hossein , Abadi Abbas Ranjbar Saadat , Alam Khan , Shukurov Karim Abdukhakimovich , Opp Christian
Published: August 2024

Dust storms are one of the important natural hazards that affect the lives of inhabitants all around the world, especially in North Africa and the Middle East. In this study, wind speed, wind direction, and air temperature patterns are investigated in one of the dustiest cities in Sistan Basin, Zahedan City, located in southeast Iran, over a 17-year period (2004–2020) using a WRF model and ground observation data. The city is located near a dust source and is mostly affected by local dust storms. The World Meteorology Organization (WMO) dust-related codes show that the city was affected by local dust, with 52 percent of the total dust events occurring during the period (2004–2021). The city’s weather station reported that 17.5% and 43% were the minimum and maximum dusty days, respectively, during 2004–2021. The summer and July were considered the dustiest season and month in the city. Since air temperature, wind speed, and wind direction are important factors in dust rising and propagation, these meteorological factors were simulated using the Weather Research and Forecasting (WRF) model for the Zahedan weather station. The WRF model’s output was found to be highly correlated with the station data; however, the WRF simulation mostly overestimated when compared with station data during the study period (2004–2020). The model had a reasonable performance in wind class frequency distribution at the station, demonstrating that 42.6% of the wind was between 0.5 and 2, which is in good agreement with the station data (42% in the range of 0.5–2). So, the WRF model effectively simulated the wind class frequency distribution and the wind direction at Zahedan station, despite overestimating the wind speed as well as minimum, maximum, and average air temperatures during the 17-year period.
An Integrated Integrable Hierarchy Arising from a Broadened Ablowitz–Kaup–Newell–Segur Scenario
Authors: Ma Wen-Xiu
Published: August 2024

This study introduces a 4×4 matrix eigenvalue problem and develops an integrable hierarchy with a bi-Hamiltonian structure. Integrability is ensured by the zero-curvature condition, while the Hamiltonian structure is supported by the trace identity. Explicit derivations yield second-order and third-order integrable equations, illustrating the integrable hierarchy.
Three-Dimensional Printing Using Biomass–Fungi Composite Materials: Brief Retrospective and Prospective Views
Authors: Pei Zhijian , Rahman Al Mazedur , Shaw Brian D. , Bedsole Caleb Oliver
Published: August 2024

Petroleum-derived plastic materials are used to manufacture a wide range of products [...]
Variability in Alignment and Bone Resections in Robotically Balanced Total Knee Arthroplasties
Authors: Hepinstall Matthew S. , Di Gangi Catherine , Oakley Christian , Sybert Michael , Meere Patrick A. , Meftah Morteza
Published: August 2024

Image-based robotic-assisted total knee arthroplasty (RA-TKA) allows three-dimensional surgical planning informed by osseous anatomy, with intraoperative adjustment based on a dynamic assessment of ligament laxity and gap balance. The aim of this study was to identify ranges of implant alignment and bone resections with RA-TKA. We retrospectively reviewed 484 primary RA-TKA cases, stratified by preoperative coronal alignment. Demographics and intraoperative data were collected and compared using Chi-square and ANOVA tests. Planned limb, femoral, and tibial alignment became increasingly varus in a progressive order from valgus to neutral to the highest in varus knees (p < 0.001). Planned external transverse rotation relative to the TEA was lowest in the valgus cohort; relative to the PCA, whereas the varus cohort was highest (p < 0.001, both). Planned resections of the lateral distal femur and of the medial posterior femur were greater in the varus group compared to neutral and valgus (p < 0.001). There were significant differences between cohorts in planned tibia resections, laterally and medially. Varus knees demonstrated higher variability, while valgus and neutral had more metrics with low variability. This study demonstrated trends in intraoperative planned alignment and resection metrics across various preoperative coronal knee alignments. These findings contribute to the understanding of RA-TKA and may inform surgical decision-making.
Remote Symptom Alerts and Patient-Reported Outcomes (PROS) in Real-World Breast Cancer Practice: Innovative Data to Derive Symptom Burden and Quality of Life
Authors: Rusli Emelly , Wujcik Debra , Galaznik Aaron
Published: August 2024

Treatment for breast cancer (BC) can lead to debilitating symptoms that can reduce outcomes and quality of life (QoL). Symptom surveillance using a remote symptom monitoring (RSM) platform enables the capture and reporting of patient-reported outcomes (PROs) from home. Women with BC used an RSM platform to complete weekly surveys and report any symptoms experienced during treatment. Symptoms reported as moderate/severe generated alerts to the clinical team. Clinical actions in response to the alert were captured. Results highlighted the value of data generated from a PRO-generated alert system to characterize longitudinal symptom burden and QoL in real-world BC practice, particularly in patients with poor functional status. The most prevalent symptoms that resulted in alerts were pain, nausea/vomiting, neuropathy, fatigue, and constipation. Most women reported one or more moderate/severe symptoms that generated an alert with an average of two alerts per week. Patients with frail status had more alerts, worse QoL and higher treatment bother, indicating that frail patients may benefit from continuous monitoring of symptoms, function, and QoL over time. A case study of patients without pre-existing peripheral neuropathy showed the rapid trajectory from the first report of mild neuropathy until alerts were generated, making a case for early intervention.
Auditory Noise Facilitates Lower Visual Reaction Times in Humans
Authors: Pérez-Pacheco Argelia , Rodríguez Morales Fernando Yael , Misaghian Khashayar , Faubert Jocelyn , Lugo Arce Jesus Eduardo
Published: August 2024

Noise is commonly seen as a disturbance but can influence any system it interacts with. This influence may not always be desirable, but sometimes it can improve the system’s performance. For example, stochastic resonance is a phenomenon where adding the right amount of noise to a weak signal makes it easier to detect. This is known as sub-threshold detection. This sub-threshold detection’s natural fingerprint is the fact that the threshold values follow an inverse U-shaped curve as the noise intensity increases. The minimum threshold value is the point of maximum sensitivity and represents the optimal point that divides the dynamics in two. Below that point, we can find the beneficial noise branch, where the noise can facilitate better detection. Above that point, the common detrimental noise concept can be found: adding noise hinders signal detection. The nervous system controls the movements and bodily functions in the human body. By reducing the sensory thresholds, we can improve the balance of these functions. Additionally, researchers have wondered if noise could be applied to different senses or motor mechanisms to enhance our abilities. In this work, noise is used to improve human reaction times. We tested the hypothesis that visual reaction times decrease significantly when the subject’s perception is in the beneficial noise branch and closer to the optimal point than outside of this condition. Auditory noise was introduced in 101 human subjects using an interface capable of searching for the right amount of noise to place the subject in the beneficial noise branch close to the optimal point. When comparing the results, the reaction times decreased when the subjects were at the optimal point compared to when the subjects were outside of such conditions. These results reveal the possibility of using this approach to enhance human performance in tasks requiring faster reaction times, such as sports.
Mutations and Differential Transcription of Mating-Type and Pheromone Receptor Genes in Hirsutella sinensis and the Natural Cordyceps sinensis Insect-Fungi Complex
Authors: Li Xiu-Zhang , Xiao Meng-Jun , Li Yu-Ling , Gao Ling , Zhu Jia-Shi
Published: August 2024

Sexual reproduction in ascomycetes is controlled by the mating-type (MAT) locus. (Pseudo)homothallic reproduction has been hypothesized on the basis of genetic data from Hirsutella sinensis (Genotype #1 of Ophiocordyceps sinensis). However, the differential occurrence and differential transcription of mating-type genes in the MAT1-1 and MAT1-2 idiomorphs were found in the genome and transcriptome assemblies of H. sinensis, and the introns of the MAT1-2-1 transcript were alternatively spliced with an unspliced intron I that contains stop codons. These findings reveal that O. sinensis reproduction is controlled at the genetic, transcriptional, and coupled transcriptional-translational levels. This study revealed that mutant mating proteins could potentially have various secondary structures. Differential occurrence and transcription of the a-/α-pheromone receptor genes were also found in H. sinensis. The data were inconsistent with self-fertilization under (pseudo)homothallism but suggest the self-sterility of H. sinensis and the requirement of mating partners to achieve O. sinensis sexual outcrossing under heterothallism or hybridization. Although consistent occurrence and transcription of the mating-type genes of both the MAT1-1 and MAT1-2 idiomorphs have been reported in natural and cultivated Cordyceps sinensis insect-fungi complexes, the mutant MAT1-1-1 and α-pheromone receptor transcripts in natural C. sinensis result in N-terminal or middle-truncated proteins with significantly altered overall hydrophobicity and secondary structures of the proteins, suggesting heterogeneous fungal source(s) of the proteins and hybridization reproduction because of the co-occurrence of multiple genomically independent genotypes of O. sinensis and >90 fungal species in natural C. sinensis.
Alterations in the Hypothalamic–Pituitary–Adrenal Axis as a Response to Experimental Autoimmune Encephalomyelitis in Dark Agouti Rats of Both Sexes
Authors: Milosevic Ana , Milosevic Katarina , Zivkovic Anica , Lavrnja Irena , Savic Danijela , Bjelobaba Ivana , Janjic Marija M.
Published: August 2024

Multiple sclerosis (MS) is a chronic inflammatory disease that affects the central nervous system, usually diagnosed during the reproductive period. Both MS and its commonly used animal model, experimental autoimmune encephalomyelitis (EAE), exhibit sex-specific features regarding disease progression and disturbances in the neuroendocrine and endocrine systems. This study investigates the hypothalamic–pituitary–adrenal (HPA) axis response of male and female Dark Agouti rats during EAE. At the onset of EAE, Crh expression in the hypothalamus of both sexes is decreased, while males show reduced plasma adrenocorticotropic hormone levels. Adrenal gland activity is increased during EAE in both males and females, as evidenced by enlarged adrenal glands and increased StAR gene and protein expression. However, only male rats show increased serum and adrenal corticosterone levels, and an increased volume of the adrenal cortex. Adrenal 3β-HSD protein and progesterone levels are elevated in males only. Serum progesterone levels of male rats are also increased, although testicular progesterone levels are decreased during the disease, implying that the adrenal gland is the source of elevated serum progesterone levels in males. Our results demonstrate a sex difference in the response of the HPA axis at the adrenal level, with male rats showing a more pronounced induction during EAE.
The Effects of Hypoxia on the Immune–Metabolic Interplay in Liver Cancer
Authors: He Yubei , Xu Han , Liu Yu , Kempa Stefan , Vechiatto Carolina , Schmidt Robin , Yilmaz Emine Yaren , Heidemann Luisa , Schnorr Jörg , Metzkow Susanne , Schellenberger Eyk , Häckel Akvile , Patzak Andreas , Müller Dominik N. , Savic Lynn Jeanette
Published: August 2024

M2-like macrophages promote tumor growth and cancer immune evasion. This study used an in vitro model to investigate how hypoxia and tumor metabolism affect macrophage polarization. Liver cancer cells (HepG2 and VX2) and macrophages (THP1) were cultured under hypoxic (0.1% O2) and normoxic (21% O2) conditions with varying glucose levels (2 g/L or 4.5 g/L). Viability assays and extracellular pH (pHe) measurements were conducted over 96 hours. Macrophages were exposed to the tumor-conditioned medium (TCM) from the cancer cells, and polarization was assessed using arginase and nitrite assays. GC-MS-based metabolic profiling quantified TCM meta-bolites and correlated them with M2 polarization. The results showed that pHe in TCMs decreased more under hypoxia than normoxia (p < 0.0001), independent of glucose levels. The arginase assay showed hypoxia significantly induced the M2 polarization of macrophages (control group: p = 0.0120,0.1%VX2-TCM group: p = 0.0149, 0.1%HepG2-TCM group: p < 0.0001, 0.1%VX2-TCMHG group: p = 0.0001, and 0.1%HepG2-TCMHG group: p < 0.0001). TCMs also induced M2 polarization under normoxic conditions, but the strongest M2 polarization occurred when both tumor cells and macrophages were incubated under hypoxia with high glucose levels. Metabolomics revealed that several metabolites, particularly lactate, were correlated with hypoxia and M2 polarization. Under normoxia, elevated 2-amino-butanoic acid (2A-BA) strongly correlated with M2 polarization. These findings suggest that targeting tumor hypoxia could mitigate immune evasion in liver tumors. Lactate drives acidity in hypoxic tumors, while 2A-BA could be a therapeutic target for overcoming immunosuppression in normoxic conditions.
Facilities in Molecular Biomarkers in Cardiology
Authors: Scicchitano Pietro , Cameli Matteo
Published: August 2024

This Special Issue of Biomolecules, entitled “Molecular Biomarkers in Cardiology 2022–2023”, presents a comprehensive collection of research and reviews exploring the rapidly evolving field of cardiovascular biomarkers [...]
Advancement and Potential Applications of Epididymal Organoids
Authors: Nie Junyu , Chen Hao , Zhao Xiuling
Published: August 2024

The epididymis, a key reproductive organ, is crucial for sperm concentration, maturation, and storage. Despite a comprehensive understanding of many of its functions, several aspects of the complex processes within the epididymis remain obscure. Dysfunction in this organ is intricately connected to the formation of the microenvironment, disruptions in sperm maturation, and the progression of male infertility. Thus, elucidating the functional mechanisms of the epididymal epithelium is imperative. Given the variety of cell types present within the epididymal epithelium, utilizing a three-dimensional (3D) in vitro model provides a holistic and practical framework for exploring the multifaceted roles of the epididymis. Organoid cell culture, involving the co-cultivation of pluripotent or adult stem cells with growth factors on artificial matrix scaffolds, effectively recreates the in vivo cell growth microenvironment, thereby offering a promising avenue for studying the epididymis. The field of epididymal organoids is relatively new, with few studies focusing on their formation and even fewer detailing the generation of organoids that exhibit epididymis-specific structures and functions. Ongoing challenges in both clinical applications and mechanistic studies underscore the importance of this research. This review summarizes the established methodologies for inducing the in vitro cultivation of epididymal cells, outlines the various approaches for the development of epididymal organoids, and explores their potential applications in the field of male reproductive biology.
Facile Splint-Free Circularization of ssDNA with T4 DNA Ligase by Redesigning the Linear Substrate to Form an Intramolecular Dynamic Nick
Authors: Sun Wenhua , Hu Kunling , Liu Mengqin , Luo Jian , An Ran , Liang Xingguo
Published: August 2024

The efficient preparation of single-stranded DNA (ssDNA) rings, as a macromolecular construction approach with topological features, has aroused much interest due to the ssDNA rings’ numerous applications in biotechnology and DNA nanotechnology. However, an extra splint is essential for enzymatic circularization, and by-products of multimers are usually present at high concentrations. Here, we proposed a simple and robust strategy using permuted precursor (linear ssDNA) for circularization by forming an intramolecular dynamic nick using a part of the linear ssDNA substrate itself as the template. After the simulation of the secondary structure for desired circular ssDNA, the linear ssDNA substrate is designed to have its ends on the duplex part (≥5 bp). By using this permuted substrate with 5′-phosphate, the splint-free circularization is simply carried out by T4 DNA ligase. Very interestingly, formation of only several base pairs (2–4) flanking the nick is enough for ligation, although they form only instantaneously under ligation conditions. More significantly, the 5-bp intramolecular duplex part commonly exists in genomes or functional DNA, demonstrating the high generality of our approach. Our findings are also helpful for understanding the mechanism of enzymatic DNA ligation from the viewpoint of substrate binding.
Central Actions of Leptin Induce an Atrophic Pattern and Improves Heart Function in Lean Normoleptinemic Rats via PPARβ/δ Activation
Authors: Rubio Blanca , Pintado Cristina , Mazuecos Lorena , Benito Marina , Andrés Antonio , Gallardo Nilda
Published: August 2024

Leptin, acting centrally or peripherally, has complex effects on cardiac remodeling and heart function. We previously reported that central leptin exerts an anti-hypertrophic effect in the heart via cardiac PPARβ/δ activation. Here, we assessed the impact of central leptin administration and PPARβ/δ inhibition on cardiac function. Various cardiac properties, including QRS duration, R wave amplitude, heart rate (HR), ejection fraction (EF), end-diastolic left ventricular mass (EDLVM), end-diastolic volume (EDV), and cardiac output (CO) were analyzed. Central leptin infusion increased cardiac PPARβ/δ protein content and decreased HR, QRS duration, and R wave amplitude. These changes induced by central leptin suggested a decrease in the ventricular wall growth, which was confirmed by MRI. In fact, the EDLVM was reduced by central leptin while increased in rats co-treated with leptin and GSK0660, a selective antagonist of PPARβ/δ activity. In summary, central leptin plays a dual role in cardiac health, potentially leading to ventricular atrophy and improving heart function when PPARβ/δ signaling is intact. The protective effects of leptin are lost by PPARβ/δ inhibition, underscoring the importance of this pathway. These findings highlight the therapeutic potential of targeting leptin and PPARβ/δ pathways to combat cardiac alterations and heart failure, particularly in the context of obesity.
Effects of Interrupting Prolonged Sitting with Light-Intensity Physical Activity on Inflammatory and Cardiometabolic Risk Markers in Young Adults with Overweight and Obesity: Secondary Outcome Analyses of the SED-ACT Randomized Controlled Crossover Trial
Authors: Hoffmann Sascha W. , Schierbauer Janis , Zimmermann Paul , Voit Thomas , Grothoff Auguste , Wachsmuth Nadine B. , Rössler Andreas , Niedrist Tobias , Lackner Helmut K. , Moser Othmar
Published: August 2024

Sedentary behavior (SB) is an essential risk factor for obesity, cardiovascular disease, and type 2 diabetes. Though certain levels of physical activity (PA) may attenuate the detrimental effects of SB, the inflammatory and cardiometabolic responses involved are still not fully understood. The focus of this secondary outcome analysis was to describe how light-intensity PA snacks (LIPASs, alternate sitting and standing, walking or standing continuously) compared with uninterrupted prolonged sitting affect inflammatory and cardiometabolic risk markers. Seventeen young adults with overweight and obesity participated in this study (eight females, 23.4 ± 3.3 years, body mass index (BMI) 29.7 ± 3.8 kg/m2, glycated hemoglobin A1C (HbA1c) 5.4 ± 0.3%, body fat 31.8 ± 8.2%). Participants were randomly assigned to the following conditions which were tested during an 8 h simulated workday: uninterrupted prolonged sitting (SIT), alternate sitting and standing (SIT-STAND, 2.5 h total standing time), continuous standing (STAND), and continuous walking (1.6 km/h; WALK). Each condition also included a standardized non-relativized breakfast and lunch. Venous blood samples were obtained in a fasted state at baseline (T0), 1 h after lunch (T1) and 8 h after baseline (T2). Inflammatory and cardiometabolic risk markers included interleukin-6 (IL-6), c-reactive protein (CRP), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), triglycerides (TGs), visceral fat area (VFA), triglyceride-glucose (TyG) index, two lipid ratio measures, TG/HDL-C and TC/HDL-C, albumin, amylase (pancreatic), total protein, uric acid, and urea. We found significant changes in a broad range of certain inflammatory and cardiometabolic risk markers during the intervention phase for IL-6 (p = 0.014), TG (p = 0.012), TC (p = 0.017), HDL-C (p = 0.020), LDL-C (p = 0.021), albumin (p = 0.003), total protein (p = 0.021), and uric acid (p = 0.040) in favor of light-intensity walking compared with uninterrupted prolonged sitting, alternate sitting and standing, and continuous standing. We found no significant changes in CRP (p = 0.529), creatinine (p = 0.199), TyG (p = 0.331), and the lipid ratios TG/HDL-C (p = 0.793) and TC/HDL-C (p = 0.221) in response to the PA snack. During a simulated 8 h work environment replacement and interruption of prolonged sitting with light-intensity walking, significant positive effects on certain inflammatory and cardiometabolic risk markers were found in young adults with overweight and obesity.
PSEN2 Mutations May Mimic Frontotemporal Dementia: Two New Case Reports and a Review
Authors: Minguillón Pereiro Anxo Manuel , Quintáns Castro Beatriz , Ouro Villasante Alberto , Aldrey Vázquez José Manuel , Cortés Hernández Julia , Aramburu-Núñez Marta , Arias Gómez Manuel , Jiménez Martín Isabel , Sobrino Tomás , Pías-Peleteiro Juan Manuel
Published: August 2024

Background: Monogenic Alzheimer’s disease (AD) has severe health and socioeconomic repercussions. Its rarest cause is presenilin 2 (PSEN2) gene mutations. We present two new cases with presumed PSEN2-AD with unusual clinical and neuroimaging findings in order to provide more information on the pathophysiology and semiology of these patients. Methods: Women aged 69 and 62 years at clinical onset, marked by prominent behavioral and language dysfunction, progressing to severe dementia within three years were included. The complete study is depicted. In addition, a systematic review of the PSEN2-AD was performed. Results: Neuroimaging revealed pronounced frontal white matter hyperintensities (WMH) and frontotemporal atrophy/hypometabolism. The genetic study unveiled PSEN2 variants: c.772G>A (p.Ala258Thr) and c.1073-2_1073-1del. Both cerebrospinal fluid (CSF) and experimental blood biomarkers shouldered AD etiology. Conclusions: Prominent behavioral and language dysfunction suggesting frontotemporal dementia (FTD) may be underestimated in the literature as a clinical picture in PSEN2 mutations. Thus, it may be reasonable to include PSEN2 in genetic panels when suspecting FTDL. PSEN2 mutations may cause striking WMH, arguably related to myelin disruption induced by amyloid accumulation.
Understanding the Complex Dynamics of Immunosenescence in Multiple Sclerosis: From Pathogenesis to Treatment
Authors: Neațu Monica , Hera-Drăguț Ana , Ioniță Iulia , Jugurt Ana , Davidescu Eugenia Irene , Popescu Bogdan Ovidiu
Published: August 2024

Immunosenescence, the gradual deterioration of immune function with age, holds profound implications for our understanding and management of multiple sclerosis (MS), a chronic autoimmune disease affecting the central nervous system. Traditionally diagnosed in young adults, advancements in disease-modifying therapies and increased life expectancy have led to a growing number of older individuals with MS. This demographic shift underscores the need for a deeper investigation into how age-related alterations in immune function shape the course of MS, influencing disease progression, treatment effectiveness, and overall patient outcomes. Age-related immunosenescence involves changes such as shifts in cytokine profiles, the accumulation of senescent immune cells, and compromised immune surveillance, collectively contributing to a state known as “inflammaging”. In the context of MS, these immunological changes disturb the intricate balance between inflammatory and regulatory responses, thereby impacting mechanisms of central immune tolerance and peripheral regulation. This paper stands out by combining the most recent advancements in immunosenescence with both pathophysiological and treatment perspectives on multiple sclerosis, offering a cohesive and accessible discussion that bridges theory and practice, while also introducing novel insights into underexplored concepts such as therapy discontinuation and the latest senolytic, neuroprotective, and remyelination therapies. Enhancing our understanding of these complexities will guide tailored approaches to MS management, ultimately improving clinical outcomes for affected individuals.
Imaging Diffractometric Biosensors for Label-Free, Multi-Molecular Interaction Analysis
Authors: Reuter Cornelia , Hauswald Walter , Burgold-Voigt Sindy , Hübner Uwe , Ehricht Ralf , Weber Karina , Popp Juergen
Published: August 2024

Biosensors are used for the specific and sensitive detection of biomolecules. In conventional approaches, the suspected target molecules are bound to selected capture molecules and successful binding is indicated by additional labelling to enable optical readout. This labelling requires additional processing steps tailored to the application. While numerous label-free interaction assays exist, they often compromise on detection characteristics. In this context, we introduce a novel diffractometric biosensor, comprising a diffractive biosensor chip and an associated optical reader assembly. This innovative system can capture an entire assay, detecting various types of molecules in a label-free manner and present the results within in a single, comprehensive image. The applicability of the biosensor is assessed for the detection of viral DNA as well as proteins directly in human plasma, investigating different antigens. In our experiments, we achieve a detection limit of 4.2 pg/mm², which is comparable to other label-free optical biosensors. The simplicity and robustness of the method make it a compelling option for advancing biosensing technologies. This work contributes to the development of an imaging diffractometric biosensor with the potential for multiple applications in molecular interaction analysis.
Development of Novel Surface-Enhanced Raman Spectroscopy-Based Biosensors by Controlling the Roughness of Gold/Alumina Platforms for Highly Sensitive Detection of Pyocyanin Secreted from Pseudomonas aeruginosa
Authors: El-Said Waleed A. , Saleh Tamer S. , Al-Bogami Abdullah Saad , Wani Mohmmad Younus , Choi Jeong-woo
Published: August 2024

Pyocyanin is considered a maker of Pseudomonas aeruginosa (P. aeruginosa) infection. Pyocyanin is among the toxins released by the P. aeruginosa bacteria. Therefore, the development of a direct detection of PYO is crucial due to its importance. Among the different optical techniques, the Raman technique showed unique advantages because of its fingerprint data, no sample preparation, and high sensitivity besides its ease of use. Noble metal nanostructures were used to improve the Raman response based on the surface-enhanced Raman scattering (SERS) technique. Anodic metal oxide attracts much interest due to its unique morphology and applications. The porous metal structure provides a large surface area that could be used as a hard template for periodic nanostructure array fabrication. Porous shapes and sizes could be controlled by controlling the anodization parameters, including the anodization voltage, current, temperature, and time, besides the metal purity and the electrolyte type/concentration. The anodization of aluminum foil results in anodic aluminum oxide (AAO) formation with different roughness. Here, we will use the roughness as hotspot centers to enhance the Raman signals. Firstly, a thin film of gold was deposited to develop gold/alumina (Au/AAO) platforms and then applied as SERS-active surfaces. The morphology and roughness of the developed substrates were investigated using scanning electron microscopy (SEM) and atomic force microscopy (AFM) techniques. The Au/AAO substrates were used for monitoring pyocyanin secreted from Pseudomonas aeruginosa microorganisms based on the SERS technique. The results showed that the roughness degree affects the enhancement efficiency of this sensor. The high enhancement was obtained in the case of depositing a 30 nm layer of gold onto the second anodized substrates. The developed sensor showed high sensitivity toward pyocyanin with a limit of detection of 96 nM with a linear response over a dynamic range from 1 µM to 9 µM.
Capillary Flow-Based One-Minute Quantification of Amyloid Proteolysis
Authors: Lee Taeha , Cheong Da Yeon , Lee Kang Hyun , You Jae Hyun , Park Jinsung , Lee Gyudo
Published: August 2024

Quantifying the formation and decomposition of amyloid is a crucial issue in the development of new drugs and therapies for treating amyloidosis. The current technologies for grasping amyloid formation and decomposition include fluorescence analysis using thioflavin-T, secondary structure analysis using circular dichroism, and image analysis using atomic force microscopy or transmission electron microscopy. These technologies typically require spectroscopic devices or expensive nanoscale imaging equipment and involve lengthy analysis, which limits the rapid screening of amyloid-degrading drugs. In this study, we introduce a technology for rapidly assessing amyloid decomposition using capillary flow-based paper (CFP). Amyloid solutions exhibit gel-like physical properties due to insoluble denatured polymers, resulting in a shorter flow distance on CFP compared to pure water. Experimental conditions were established to consistently control the flow distance based on a hen-egg-white lysozyme amyloid solution. It was confirmed that as amyloid is decomposed by trypsin, the flow distance increases on the CFP. Our method is highly useful for detecting changes in the gel properties of amyloid solutions within a minute, and we anticipate its use in the rapid, large-scale screening of anti-amyloid agents in the future.
Epidemiology of Sports-Related Concussion in Japanese University Soccer Players
Authors: Fukushima Hiroshi , Shigemori Yutaka , Otsubo Shunya , Goto Kyosuke , Terada Koki , Tachihara Muneyuki , Kurosaki Tatsuma , Yamaguchi Keita , Otsuka Nana , Masuda Kentaro , Tsurusaki Rino , Inui Masahiro
Published: August 2024

In recent years, sports-related concussion (SRC) in soccer has been extensively researched worldwide. However, there have been no reports of large-scale SRC studies among soccer players in Japan. The purpose of this study is to investigate the epidemiology of SRC among university soccer players in Japan. This descriptive epidemiological study collected data on the history of SRC and details of SRC injuries during soccer. The participants were university male soccer players belonging to the Japan University Football Association. SRC rates were calculated per 1000 athlete-exposures (AEs). A total of 5953 students participated in this study. The SRC rate was 0.10/1000 AE during total activities. The SRC rate during competition (0.42/1000 AE) was higher than in practice (0.04/1000 AE). The most frequent mechanism of SRC was “head-to-head” (26.9%), followed by “head-to-ball” (24.2%). During competition, the most frequent mechanism was “head-to-head” (30.8%), followed by “head-to-ground” (23.8%), and “head-to-ball” (19.3%) followed, while in practice, it was “head-to-ball” (34.8%), followed by “head-to-ground” (23.8%), and “head-to-head” (17.2%). Thus, there was a difference in the mechanism of injury between competition and practice. In this study, among Japanese university soccer players, the SRC rate was to be approximately ten times higher in competition than in practice.
FluoAnalysis: An Open-Source MATLAB Toolbox for Analysis of Calcium Imaging Measurements of Oscillatory Astrocytic and Neuronal Networks
Authors: Péter Márton , Héja László
Published: August 2024

Calcium imaging, especially two-photon imaging, has become essential in neuroscience for studying neuronal and astrocytic activity under in vivo and in vitro conditions. Current advances in the development of calcium sensors as well as imaging hardware enable high-frequency measurements of calcium signals in hundreds of cells simultaneously. The analysis of these large datasets requires special tools and usually a certain level of programming experience. Despite advancements in calcium imaging analysis software development, significant gaps remain, particularly for data acquired at a high sampling rate that would allow for the spectral analysis of calcium signals. The FluoAnalysis MATLAB toolbox addresses these gaps by offering a comprehensive solution for analyzing simultaneously measured calcium imaging and electrophysiological data. It features both GUI-based and command-line approaches, emphasizing frequency domain analysis to reveal network-level oscillatory signals linked to single-cell activity. In addition, the toolbox puts special emphasis on differentiating between astrocytes and neurons, revealing the interactions between the network activity of the two major cell types of the brain. It facilitates a streamlined workflow for data loading, ROI identification, cell classification, fluorescence intensity calculation, spectral analysis, and report generation, supporting both manual and automated high-throughput analysis. This versatile platform enables the comprehensive analysis of large imaging datasets. In conclusion, the FluoAnalysis MATLAB toolbox provides a robust and versatile platform for the integrated analysis of calcium imaging and electrophysiological data, supporting diverse neuroscience research applications.
Expected Future Subjective Social Status Moderates the Relations between Perceived Parental Expectation and Persistence among Chinese Rural Adolescents
Authors: Zhang Feng , Yang Rui , Xu Xiaodan
Published: August 2024

Adolescents’ expectations on future subjective social status (SSS) may play a critical role in the relations between perceived parental expectations and persistence; however, there is a lack of research exploring this effect in the context of families experiencing greater economic risk. This study aimed to explicitly address this issue. A total of 698 Chinese rural adolescents participated in this study (Mage = 13.32 years; 54.60% boys). The results showed that for rural adolescents with lower expected future SSS, perceived parental expectation was negatively related to persistence; for rural adolescents with higher expected future SSS, perceived parental expectation was not significantly correlated with persistence. These findings imply the adverse effects of high perceived parental expectation on rural adolescents’ persistence and that expected future SSS can alleviate this adverse relationship.
Evaluation of the Strengthening Effects on Prestressed Carbon-Fiber-Reinforced-Polymer-Strengthened Steel Beam Bridges Using Macro-Strain Influence Lines
Authors: Wu Bitao , Xia Qingquan , Gong Yan , Fu Sicheng , Wang Haitao , Guo Zhongzhao
Published: August 2024

Effectively evaluating the effectiveness of bridge strengthening is a necessary means to ensure the normal operation of existing strengthened bridges, especially when evaluating the effectiveness of bridge strengthening without interrupting normal traffic. Based on a distributed long-gauge Fiber Bragg Grating (FBG) sensor, this paper derived the macro-strain influence line (MSIL) formula for a simply supported beam bridge under a moving vehicle load, studied the changes in the MSIL at the bottom of the beam under the vehicle load before and after the prestressed CFRP plate strengthening, and proposed a rapid evaluation method for the strengthening effect based on the amplitude of the MSIL as the evaluation index for the strengthening effect. Finally, the prestressed CFRP-strengthened steel beam was tested under the moving vehicle load. The theoretical analysis and the experimental results confirm that under the load of moving vehicles, the macro-strain–time history amplitude of the strengthened steel beams under different prestressed tensioning conditions is different. The amplitude of the macro-strain time history of the strengthened bridge is reduced compared to before strengthening, and the local strengthening effect of the bridge can be monitored by the amplitude change in a single sensor. The change in global stiffness can be evaluated by monitoring the MSIL obtained from multiple long-gauge strain sensors.