bluuebunny/crossref_metadata_2025 huggingface dataset:
Long-term risk of neoplastic events after childhood growth hormone treatment: a population-based cohort study in Sweden
Authors: Tidblad Anders , Bottai Matteo , Smedby Karin E. , Albertsson-Wikland Kerstin , Sävendahl Lars
Published: March 2024

Background

Increased risk of neoplastic events after recombinant human growth hormone (rhGH) treatment in childhood has been an ongoing concern but long-term safety data are limited.

Methods

A nationwide population-based cohort study in Sweden of patients treated with rhGH during childhood between 1985-2010, due to isolated growth hormone deficiency (GHD), small for gestational age (SGA) and idiopathic short stature (ISS). The comparison group consisted of 15 age-, sex-, and region-matched controls per patient, randomly selected from the general population. Data on neoplastic events and covariates, such as gestational age, birth weight, birth length, socioeconomic status, and height at study start, were collected through linkage with population-based registers. The cohort was followed for neoplastic events until the end of 2020.

Results

53,444 individuals (3,408 patients; 50,036 controls) were followed for up to 35 years, with a median follow-up of 19.8 years and a total of 1,050,977 person-years. Patients showed a moderately increased hazard ratio (HR) for neoplastic events overall compared to controls (HR 1.28, 95% CI: 1.12-1.46), but only significant for males (HR 1.39, 95% CI: 1.17-1.66) and not females (HR 1.15, 95% CI: 0.94-1.41). Longer treatment duration was associated with an increased HR, but no association was found between neoplastic events and mean or cumulative dose. No increased risk of malignant neoplasms was observed for the patients compared to matched controls (HR 0.91 95% CI: 0.66-1.26).

Conclusion

No association was found between rhGH treatment during childhood for GHD, SGA, or ISS and malignant neoplastic events in early to mid-adulthood. A moderate increase in overall neoplastic events was observed due to an increased number of events in male patients.
The relationship between daytime napping and glycemic control in people with type 2 diabetes
Authors: Yuan Jinjin , Wang Jinle , Chen Yingdan , Zhang Min , Zhao Aimei , Du Jing , Zhang Jiahui , Liu Fan , Wang Yueying , Chen Pei , Zhu Bingqian
Published: March 2024

Aim

To examine the association between napping characteristics and glycemic control in people with type 2 diabetes.

Design

This study used a cross-sectional design.

Methods

A convenience sample of people with type 2 diabetes (N=226) were included. Glycemic control was indicated by HbA1c which was measured by A1C Now®+. Napping characteristics including napping frequency, duration, timing, and type were measured by validated questionnaires. Other variables, such as insomnia, cognitive impairment, and depression were measured by the Insomnia Severity Index, Montreal Cognitive Assessment, and Patient Health Questionnaire-9, respectively. Multivariate linear regression analyses were performed.

Results

The sample consisted of 122 women (54.0%), with a median age of 67 years. Their median HbA1c was 6.8%. No significant relationship was found between napping frequency and HbA1c. Among nappers, after controlling for covariates, long napping duration (≥60 min) and morning napping were both associated with poorer glycemic control. Compared with appetitive napping, restorative napping was associated with better glycemic control.

Conclusion

Daytime napping (e.g., duration and type) is an important modifiable factor for glycemic control in people with type 2 diabetes. This study provides new insights into the relationship between napping and glucose management among people with diabetes.
Integrating PANoptosis insights to enhance breast cancer prognosis and therapeutic decision-making
Authors: Wang Shu , Li Zhuolin , Hou Jing , Li Xukui , Ni Qing , Wang Tao
Published: March 2024

Background

Despite advancements, breast cancer outcomes remain stagnant, highlighting the need for precise biomarkers in precision medicine. Traditional TNM staging is insufficient for identifying patients who will respond well to treatment.

Methods

Our study involved over 6,900 breast cancer patients from 14 datasets, including in-house clinical data and single-cell data from 8 patients (37,451 cells). We integrated 10 machine learning algorithms in 55 combinations and analyzed 100 existing breast cancer signatures. IHC assays were conducted for validation, and potential immunotherapies and chemotherapies were explored.

Results

We pinpointed six stable Panoptosis-related genes from multi-center cohorts, leading to a robust Panoptosis-model. This model outperformed existing clinical and molecular features in predicting recurrence and mortality risks, with high-risk patients showing worse outcomes. IHC validation from 30 patients confirmed our findings, indicating the model’s broader applicability. Additionally, the model suggested that low-risk patients benefit more from immunotherapy, while high-risk patients are sensitive to specific chemotherapies like BI-2536 and ispinesib.

Conclusion

The Panoptosis-model represents a major advancement in breast cancer prognosis and treatment personalization, offering significant insights for effectively managing a wide range of breast cancer patients.
The NF-κB RelA transcription factor is not required for CD8+ T-cell function in acute viral infection and cancer
Authors: Voisin Allison , Plaschka Maud , Perrin-Niquet Marlène , Twardowski Julie , Boutemine Insaf , Eluard Baptiste , Lalle Guilhem , Stéphan Pierre , Bouherrou Khaled , Tonon Laurie , Pommier Roxane , Ferrari Anthony , Klein Ulf , Wencker Mélanie , Baud Véronique , Cassier Philippe A. , Grinberg-Bleyer Yenkel
Published: March 2024

CD8+ T cells are critical mediators of pathogen clearance and anti-tumor immunity. Although signaling pathways leading to the activation of NF-κB transcription factors have crucial functions in the regulation of immune responses, the CD8+ T cell-autonomous roles of the different NF-κB subunits, are still unresolved. Here, we investigated the function of the ubiquitously expressed transcription factor RelA in CD8+ T-cell biology using a novel mouse model and gene-edited human cells. We found that CD8+ T cell-specific ablation of RelA markedly altered the transcriptome of ex vivo stimulated cells, but maintained the proliferative capacity of both mouse and human cells. In contrast, in vivo experiments showed that RelA deficiency did not affect the CD8+ T-cell response to acute viral infection or transplanted tumors. Our data suggest that in CD8+ T cells, RelA is dispensable for their protective activity in pathological contexts.
Spatial and seasonal pattern of microbial nitrate reduction in coastal sediments in the Vistula River plume area, Gulf of Gdańsk
Authors: Benelli Sara , Bartoli Marco , Magri Monia , Brzana Radosław , Kendzierska Halina , Styrcz-Olesiak Kamila , Janas Urszula
Published: March 2024

Estuaries can remove and/or retain land-derived nitrogen (N) and act as filters buffering N loads to the open sea. The N coastal filter can be seasonally variable depending on water temperature and transported loads, two factors acting in synergy and strongly influenced by climate change. The capacity of sediments to mitigate riverine N loads was investigated at four sites in the Vistula River plume area (Gulf of Gdańsk, Southern Baltic Sea). Samplings were carried out in two contrasting seasons: spring and summer, characterized by different water temperatures and nitrate (NO3-) levels. Inorganic N fluxes, and rates of denitrification and dissimilatory nitrate reduction to ammonium (DNRA) were measured in intact sediment cores by means of dark incubations and 15N-nitrate concentration-series experiments. Sampling sites were selected along a gradient of depth (5 to 24 m), that was also a gradient of sediment organic matter content. In both seasons, denitrification rates increased along with depth and from spring (6.5 ± 7.0 µmol m-2 h-1) to summer (20.4 ± 15.4 µmol m-2 h-1), despite lower NO3- concentrations in summer. In spring, at higher NO3- loading, denitrification was likely limited by low water temperature, and elevated sediment oxygen penetration. Coupled denitrification-nitrification prevailed over denitrification of water column NO3- across all sites and seasons, contributing to over 80% of the total denitrification. Notably, no anammox was detected at the sampling sites. DNRA exhibited low to undetectable rates in spring, especially at the shallowest sites. However, during summer, N recycling via DNRA increased and ranged from 0.7 to 14.9 µmol m-2 h-1. The denitrification efficiency (DE), calculated as the ratio between molecular nitrogen (N2) flux and dissolved inorganic N effluxes from sediments, ranged from 0 to 37% in spring, whereas in summer DE did not exceed 16%. Despite the dominance of denitrification over DNRA, the analyzed sediments acted as weak N buffers under in situ dark conditions. However, concentration-series experiments suggested high potential denitrification capacity, exceeding 400 µmol m-2 h-1, in response to short-term, large riverine inputs of NO3-.
Brownification in the Eastern Mediterranean Sea: effect of simulated terrestrial input on the planktonic microbial food web in an oligotrophic sea
Authors: Ktistaki Georgia , Magiopoulos Iordanis , Corno Gianluca , Courboulès Justine , Eckert Ester M. , González Jose , Kalantzi Ioanna , Middelboe Mathias , Symiakaki Katerina , Tsapakis Manolis , Vidussi Francesca , Pitta Paraskevi
Published: March 2024

Terrestrial input to marine and freshwater ecosystems colors the water yellow-brown, causing a phenomenon called “brownification”. The effect of brownification on the marine pelagic microbial food web was studied in the oligotrophic eastern Mediterranean in June 2021 by adding HuminFeed in a 15-day mesocosm experiment with 2 treatments: Control (C, no addition) and HuminFeed (HF, single dose of HuminFeed, 2 mg L-1); and 3 replicates per treatment. HuminFeed caused shading, leading to a decrease in the abundance of photo-autotrophic organisms (cyanobacteriaSynechococcusand diatoms). Bacteria were positively affected by the HF addition (mainly in terms of production rather than abundance), benefiting either directly from the dissolved organic carbon (DOC) contained in HuminFeed or indirectly from the trophic cascade through the food web. Despite the decrease in HF bacterial abundance during the experiment, an increase in both the high nucleic acid containing bacteria% and heterotrophic bacterial production were observed, suggesting higher activity at the single cell level. In the HF treatment, the increased abundance of dinoflagellates observed could be due to either a dominance of mixotrophic species or a release from predation by copepods. Both ciliates and copepods were severely impacted by HuminFeed, showing lower abundance and distorted forms (ciliates) and reduced reproductive potential (copepods). In conclusion, in the ultraoligotrophic eastern Mediterranean, the simulated brownification negatively affected autotrophs and top predators while benefiting bacteria, thus indicating a shift in the structure of the plankton food web.
The association between chronic obstructive pulmonary disease and autoimmune diseases: a bidirectional Mendelian randomization study
Authors: Yu Xiaohui , Cheng Xue , Lv Lin , Wang Na , Li Mengcong , Ji Wenwen , Liu Tingting , Wang Guangdong , Hu Tinghua , Shi Zhihong
Published: March 2024

Objective

Observational studies have reported that chronic obstructive pulmonary disease (COPD) is often accompanied by autoimmune diseases, but the causal relationships between them remain uncertain. In this Mendelian study, we aimed to investigate the potential causal relationship between COPD and four common autoimmune diseases.

Methods

We conducted an analysis of summary data on COPD and autoimmune disease using publicly available genome-wide association studies (GWAS) summary data. We initially employed the inverse- variance weighted method as the primary approach to establish the causal impact of COPD on autoimmune diseases in the sample and conducted additional sensitivity analyses to examine the robustness of the results. Subsequently, we performed reverse Mendelian randomization (MR) analyses for the four autoimmune diseases. Finally, the potential for bidirectional causal relationships was assessed.

Results

Our MR analysis revealed no significant causal relationship between COPD and any of the studied autoimmune diseases. However, reverse MR results indicated a significant association between rheumatoid arthritis (RA), osteoarthritis (OA) and the risk of developing COPD, with respective odds ratios (OR) of 377.313 (95% CI, 6.625–21487.932, P = 0.004) for RA and 11.097 (95% CI, 1.583–77.796, P = 0.015) for OA. Sensitivity analyses confirmed the robustness of the results.

Conclusion

Our findings support a potential causal relationship between autoimmune diseases and COPD, highlighting the importance of considering comorbidities in clinical management of COPD.
Causal relationship between atrial fibrillation/warfarin and cutaneous melanoma: a two-sample Mendelian randomization study
Authors: Xiaowu Wang , Qiang Zhou , Yike Han , Wu Zhixuan , Yiheng Jin , Xuemei Chen , Sen Lin , Jiong Chen
Published: March 2024

Purpose

In recent years, the relationship between malignant tumors and atrial fibrillation has attracted more and more attention. Atrial fibrillation can also cause a series of adverse events, such as the risk of thromboembolism. Also, Warfarin is often used here. But, the relationship between cutaneous melanoma and atrial fibrillation, and between cutaneous melanoma and warfarin is still unclear. Therefore, we used a two-sample Mendelian randomization to assess the causal relationship between atrial fibrillation/warfarin and cutaneous melanoma (cM).

Methods

Firstly, atrial fibrillation (ukb-b-11550; nCase = 3,518, nControl = 459,415) and warfarin (ukb-b-13248; nCase = 4,623, nControl = 458,310) as exposures, with genome-wide association studies (GWAS) data from the United Kingdom Biobank. And cM (ieu-b-4969; nCase = 3,751, nControl = 372,016) as outcome, with GWAS data from the IEU Open GWAS project. Subsequently, single-nucleotide polymorphisms (SNPs) were filtered from GWAS studies using quality control measures. In addition, two-sample Mendelian randomization (MR) analysis was performed to explore the causal relationship between atrial fibrillation or warfarin and cM and used inverse variance weighting (IVW) as the primary analytical method. Finally, relevant heterogeneity and sensitivity analysis were performed to ensure the accuracy of the results.

Results

A causal relationship between atrial fibrillation and cutaneous melanoma was observed, and between warfarin and cutaneous melanoma.

Conclusion

The atrial fibrillation may play a causal role in the development of cutaneous melanoma, but the mechanism and the causal relationship between warfarin and cutaneous melanoma needs to be further elucidated.
Activities of aztreonam in combination with several novel β-lactam-β-lactamase inhibitor combinations against carbapenem-resistant Klebsiella pneumoniae strains coproducing KPC and NDM
Authors: Li Xinhui , Zhang Jisheng , Wang Jianmin , Long Wenzhang , Liang Xushan , Yang Yang , Gong Xue , Li Jie , Liu Longjin , Zhang Xiaoli
Published: March 2024

Isolates coproducing serine/metallo-carbapenems are a serious emerging public health threat, given their rapid dissemination and the limited number of treatment options. The purposes of this study were to evaluate the in vitro antibacterial activity of novel β-lactam-β-lactamase inhibitor combinations (BLBLIs) against carbapenem-resistant Klebsiella pneumoniae (CRKP) coproducing metallo-β-lactamase and serine-β-lactamase, and to explore their effects in combination with aztreonam, meropenem, or polymyxin in order to identify the best therapeutic options. Four CRKP isolates coproducing K. pneumoniae carbapenemase (KPC) and New Delhi metallo-β-lactamase (NDM) were selected, and a microdilution broth method was used to determine their susceptibility to antibiotics. Time-kill assay was used to detect the bactericidal effects of the combinations of antibiotics. The minimum inhibitory concentration (MIC) values for imipenem and meropenem in three isolates did not decrease after the addition of relebactam or varbobactam, but the addition of avibactam to aztreonam reduced the MIC by more than 64-fold. Time-kill assay demonstrated that imipenem-cilastatin/relebactam (ICR) alone exerted a bacteriostatic effect against three isolates (average reduction: 1.88 log10 CFU/mL) and ICR combined with aztreonam exerted an additive effect. Aztreonam combined with meropenem/varbobactam (MEV) or ceftazidime/avibactam (CZA) showed synergistic effects, while the effect of aztreonam combined with CZA was inferior to that of MEV. Compared with the same concentration of aztreonam plus CZA combination, aztreonam/avibactam had a better bactericidal effect (24 h bacterial count reduction >3 log10CFU/mL). These data indicate that the combination of ATM with several new BLBLIs exerts powerful bactericidal activity, which suggests that these double β-lactam combinations might provide potential alternative treatments for infections caused by pathogens coproducing-serine/metallo-carbapenems.
New layers of regulation of the general stress response sigma factor RpoS
Authors: Handler Simon , Kirkpatrick Clare L.
Published: March 2024

The general stress response (GSR) sigma factor RpoS from Escherichia coli has emerged as one of the key paradigms for study of how numerous signal inputs are accepted at multiple levels into a single pathway for regulation of gene expression output. While many studies have elucidated the key pathways controlling the production and activity of this sigma factor, recent discoveries have uncovered still more regulatory mechanisms which feed into the network. Moreover, while the regulon of this sigma factor comprises a large proportion of the E. coli genome, the downstream expression levels of all the RpoS target genes are not identically affected by RpoS upregulation but respond heterogeneously, both within and between cells. This minireview highlights the most recent developments in our understanding of RpoS regulation and expression, in particular those which influence the regulatory network at different levels from previously well-studied pathways.
Profiling of Petroselinum sativum (mill.) fuss phytoconstituents and assessment of their biocompatibility, antioxidant, anti-aging, wound healing, and antibacterial activities
Authors: Mahdi Ismail , Imbimbo Paola , Annaz Hassan , Bakrim Widad Ben , Sahri Nihad , Alaoui Asmae , Monti Daria Maria , Sobeh Mansour
Published: March 2024

Petroselinum sativum, known as parsley, is a fragrant herb that possesses a rich heritage of utilization in traditional medicinal practices. In this study, we annotated the phytocontents of the aqueous and ethanolic extracts of P. sativum and investigated their antioxidant, cytoprotective, antiaging, wound healing, and antibacterial activities. LC–MS/MS analysis of both extracts revealed the presence of 47 compounds belonging to diverse groups including organic acids, phenolic acids, and flavonoids. By MTT assay, the extracts were fully biocompatible on immortalized human keratinocytes (HaCaT) while they inhibited intracellular ROS formation (DCFDA assay) and prevented GSH depletion (DTNB assay) upon UVA exposure. In addition, the extracts were potent in inhibiting the in vitro activities of skin-related enzymes mainly elastase, tyrosinase, collagenase and hyaluronidase. Using the scratch assay, P. sativum aqueous extract significantly enhanced wound closure when compared to untreated HaCaT cells. Moreover, both extracts inhibited Pseudomonas aeruginosa’s growth, reduced biofilm formation, and impaired the swimming and swarming motilities. Also, the aqueous extract was able to inhibit the production of bacterial pigments on plates. These findings strongly suggest the usefulness of P. sativum as a source of phytochemicals suitable for dermo-cosmeceutical applications.
Prospective observational study of nutritional status and oral supplement utilization in users of an elderly daycare service, employing a web-based Mini Nutritional Assessment Form (MNA plus)
Authors: Takano Hiroki , Kamada Yukikazu , Ichikawa Masaki , Yoshida Sadao
Published: March 2024

Introduction

Seniors are vulnerable to frailty, a condition linked to falls, fractures, hospitalizations, and sarcopenia. Even with regular meals, senior daycare users are at risk for malnutrition.

Methods

This study assessed malnutrition risk in daycare users, using the web-based Mini Nutritional Assessment Form (MNA®-SF). Individuals identified as malnourished or at risk were examined for changes in nutritional status with and without oral nutritional supplementation (ONS).

Results

Of 507 subjects, 138 (27.2%) were malnourished or at risk. Discontinuation rates were 20.0% (7/35) for the ONS group and 40.0% (10/25) for the regular care (RC) group. Among 29 patients with measurable weight change after six months, 19 (ONS group) and 10 (RC group) participated. The ONS group exhibited significant increases in body weight (+1.4 ± 2.9 kg, p < 0.01), body mass index (BMI) (+0.6 ± 0.9 kg/m2, p < 0.01), calf circumference (+3.2 ± 0.2 cm, p < 0.01), and grip strength (+1.2 ± 1.9 kg, p = 0.069). Conversely, the RC group showed no significant increases in body weight (+1.0 ± 1.9 kg, p = 0.146), BMI (+0.4 ± 0.8 kg/m2, p = 0.176), or grip strength (−0.7 ± 1.7 kg, p = 0.327), with decreased grip strength and calf circumference (−0.8 ± 0.9 cm, p < 0.05). In the ONS group, 52.6% (10/19) consumed over 400 kcal/day of ONS, and 84.2% maintained this intake for three months. Malnutrition is prevalent among daycare users.

Conclusion

ONS influences weight, BMI, and calf circumference, potentially reducing discontinuation rates.

Clinical trial registration

https://center6.umin.ac.jp/cgi-open-bin/ctr_e/ctr_view.cgi?recptno=R000049767, UMIN000043580.
Case Report: Identification of a CARD8 variant in all three patients with PFAPA syndrome complicated with Kawasaki disease
Authors: Nakamura Haruhiko , Kikuchi Atsuo , Sakai Hideyuki , Kamimura Miki , Watanabe Yohei , Onuma Ryoichi , Takayama Jun , Tamiya Gen , Mashimo Yoichi , Ebata Ryota , Hamada Hiromichi , Suenaga Tomohiro , Onouchi Yoshihiro , Kumaki Satoru
Published: March 2024

Background

Periodic fever, aphthous stomatitis, pharyngitis, and cervical adenitis (PFAPA syndrome), and Kawasaki disease (KD) are both considered to be disorders of the innate immune system, and the potential role of inflammasome activation in the immunopathogenesis of both diseases has been previously described.

Case presentation

Herein, we report the clinical courses of three patients who presented a rare combination of PFAPA syndrome and KD. Two patients who presented KD later developed the PFAPA syndrome, of whom one developed recurrent KD 2 years after the initial diagnosis. The third patient developed KD one year after the onset of PFAPA syndrome. The presence of both of these conditions within individual patients, combined with the knowledge that inflammasome activation is involved in both PFAPA syndrome and KD, suggests a shared background of inflammatory dysregulation. To elucidate the mechanism underlying shared inflammatory dysregulation, we investigated the roles of Nod-like receptors (NLRs) and their downstream inflammasome-related genes. All the patients had a frameshift variant in CARD8 (CARD8-FS). A previous study demonstrated a higher frequency of CARD8-FS, whose product loses CARD8 activity and activates the NLRP3 inflammasome, in patients with the PFAPA syndrome. Additionally, the NLRP3 inflammasome is known to be activated in patients with KD. Together, these results suggest that the CARD8-FS variant may also be essential in KD pathogenesis. As such, we analyzed the CARD8 variants among patients with KD. However, we found no difference in the variant frequency between patients with KD and the general Japanese population.

Conclusions

We report the clinical courses of three patients with a rare combination of PFAPA syndrome and KD. All the patients had the CARD8-FS variant. However, we could not find a difference in the variant frequency between patients with KD and the general Japanese population. As the frequency of KD is much higher than that of PFAPA among Japanese patients, and the cause of KD is multifactorial, it is possible that only a small portion of patients with KD harbor CARD8-FS as a causative gene.
Synaptic mechanisms underlying the elevated sympathetic outflow in fructose-induced hypertension
Authors: Zhu Yun , Sun Haiying , Wang Hongjie , Li Na
Published: March 2024

Metabolic syndrome is associated with cardiovascular dysfunction, including elevated sympathetic outflow. However, the underlying brain mechanisms are unclear. The nucleus tractus solitarius (NTS) critically regulates autonomic reflexes related to cardiovascular function and contains neurons projecting to the caudal ventrolateral medulla (CVLM). Nitric oxide (NO) is a diffusible free-radical messenger in the vascular, immune, and nervous systems. In this study, we determine if NO in the NTS is involved in the synaptic plasticity underlying the elevated sympathetic outflow in fructose-induced hypertension. We retrogradely labeled CVLM-projecting NTS neurons through the injection of FluoSpheres into the CVLM in a fructose-fed rat model to determine the cellular mechanism involved in increased sympathetic outflow. Fructose feeding increased the blood pressure and glucose levels, which represent metabolic syndrome. We found that fructose feeding reduces the NO precursor L-arginine-induced increase in the firing activity of CVLM-projecting NTS neurons. Furthermore, fructose feeding reduces the L-arginine-induced increase in presynaptic spontaneous glutamatergic synaptic inputs to NTS neurons, while NO donor DEA/NO produces an increase in glutamatergic synaptic inputs in fructose-fed rats similar to that in vehicle-treated rats. In addition, fructose feeding reduces the NO-induced depressor response and sympathoinhibition. These data suggested that fructose feeding reduced NO production and, thus, the subsequent NO-induced glutamate releases in the NTS and depressor response. The findings of this study provide new insights into the central mechanisms involved in the neural control of cardiovascular and autonomic functions in the NTS in metabolic syndrome.
Disease-resistant varieties of Chinese cabbage (Brassica rapa L. ssp. pekinensis) inhibit Plasmodiophora brassicae infestation by stabilising root flora structure
Authors: Fang Tianyi , Han Xueyu , Yue Yanling
Published: March 2024

The application of disease-resistant varieties is the most cost-effective method for solving the problem of clubroot. “Shangpin,” a disease-resistant variety of Chinese cabbage with broad-spectrum immunity to Plasmodiophora brassicae (P. brassicae), was screened in a previous study. Based on 16S rRNA sequencing technology, we annotated the compositional differences between the rhizosphere, rhizoplane, and endosphere bacterial communities of “Shangpin” and “83-1” under P. brassicae stress. Alpha diversity analysis showed that the abundance of microorganisms in the root system of “83-1” changed more than that of “Shangpin” after P. brassicae infestation, and Beta diversity analysis indicated that Flavobacterium and Sphingomonas may mediate clubroot resistance, while Nitrospira, Nitrosospira, and Pseudomonas may mediate P. brassicae infestation among the bacteria in the Top 10 abundances. Microbial functional analyses showed that the root microorganisms of “83-1” were metabolically weakened after P. brassicae inoculation and were inhibited in competition with pathogenic bacteria. Conversely, the root microorganisms of “Shangpin” maintained the strength of their metabolic capacity, which took a favorable position in competition with the pathogen and inhibited the growth and development of the pathogen, thus showing resistance. Root secretions of “Shangpin” significantly inhibited the incidence and disease index of clubroot, which indicated that under clubroot stress, resistant varieties maintain root microbial diversity and microbial community functions through specific root exudates, enriching the genera Flavobacterium and Sphingomonas, thus showing resistance. The results of this study reveal the resistance mechanism of resistant varieties to clubroot and provide new insights into the prevention and control of clubroot in Chinese cabbage.
Quantitative analysis of MBW complex formation in the context of trichome patterning
Authors: Zhang Bipei , Deneer Anna , Fleck Christian , Hülskamp Martin
Published: March 2024

Trichome patterning in Arabidopsis is regulated by R2R3MYB, bHLH and WDR (MBW) genes. These are considered to form a trimeric MBW protein complex that promotes trichome formation. The MBW proteins are engaged in a regulatory network to select trichome cells among epidermal cells through R3MYB proteins that can move between cells and repress the MBW complex by competitive binding with the R2R3MYB to the bHLHL protein. We use quantitative pull-down assays to determine the relative dissociation constants for the protein-protein interactions of the involved genes. We find similar binding strength between the trichome promoting genes and weaker binding of the R3MYB inhibitors. We used the dissociation constants to calculate the relative percentage of all possible complex combinations and found surprisingly low fractions of those complexes that are typically considered to be relevant for the regulation events. Finally, we predict an increased robustness in patterning as a consequence of higher ordered complexes mediated by GL3 dimerization.
Chitin-enriched insect frass fertilizer as a biorational alternative for root-knot nematode (Meloidogyne incognita) management
Authors: Kisaakye James , Beesigamukama Dennis , Haukeland Solveig , Subramanian Sevgan , Thiongo Paul K. , Kelemu Segenet , Tanga Chrysantus M.
Published: March 2024

Root-knot nematodes (Meloidogyne spp.) are serious pests of most food crops, causing up to 100% yield loss. Nevertheless, commercial nematicides are costly and harmful to the environment. While the nematicidal potential of crustacean and synthetic chitin has been demonstrated globally, research on the potential of insect-derived chitin for nematode control has received limited attention. Here, seven chitin-fortified black soldier fly frass fertilizer extracts (chFE) were assessed for their suppressiveness of Meloidogyne incognita and impacts on spinach growth in comparison with a commercial nematicide using in vitro and in vivo bioassays. The performance of chFE and control treatments was assessed by determining their effects on nematode egg hatchability; infective juvenile (J2) mortality and paralysis; number of galls, egg masses, and J2s per plant; and spinach root and shoot biomass. In vitro results showed that chFE and commercial nematicide suppressed nematode egg hatchability by 42% and 52%, respectively, relative to the control (sterile distilled water). Up to 100% paralysis was achieved when M. incognita J2s were exposed to either chFE or commercial nematicide. Further, the J2 mortality achieved using chFE (95%) was comparable to the value achieved using commercial nematicide (96%); in all treatments, mortality increased with exposure time. Similarly, up to 85% suppression of gall development was achieved when spinach plants were grown in soil drenched with chFE; up to 79% reduction in egg mass formation and 68% suppression of J2 development in the root system were achieved using chFE. Also, chFE application significantly increased spinach root and shoot biomass by 54%–74% and 39%–58%, respectively, compared to commercial nematicide. Our findings demonstrate the nematicidal potential of chFE and its benefits on crop production. Thus, chFE could be considered as a promising multipurpose, regenerative, and cost-effective input for sustainable management of plant-parasitic nematodes and enhancement of crop yield.
Effect of extracellular polymeric substances on the colony size and morphological changes of Microcystis
Authors: Pan Jiaxin , Yang Zhongyong , Hu Nan , Xiao Bangding , Wang Chunbo , Wu Xingqiang , Yang Tiantian
Published: March 2024

Surface blooms of colony-forming Microcystis are increasingly occurring in aquatic ecosystems on a global scale. Recent studies have found that the Microcystis colonial morphology is a crucial factor in the occurrence, persistence, and dominance of Microcystis blooms, yet the mechanism driving its morphological dynamics has remained unknown. This study conducted a laboratory experiment to test the effect of extracellular polymeric substances on the morphological dynamics of Microcystis. Ultrasound was used to disaggregate colonies, isolating the cells and of the Microcystis suspension. The single cells were then re-cultured under three homologous EPS concentrations: group CK, group Low, and group High. The size, morphology, and EPS [including tightly bound EPS (TB-EPS), loosely bound EPS (LB-EPS), bound polysaccharides (B-polysaccharides), and bound proteins (B-proteins)] changes of colonies were closely monitored over a period of 2 months. It was observed that colonies were rapidly formed in group CK, with median colony size (D50) reaching 183 µm on day 12. The proportion of colonies with a size of 150–500 µm increased from 1% to more than 50%. Colony formation was also observed in both groups Low and High, but their D50 increased at a slower rate and remained around 130 µm after day 17. Colonies with a size of 50–150 µm account for more than 50%. Groups CK and Low successively recovered the initial Microcystis morphology, which is a ring structure formed of several small colonies with a D50 of 130 µm. During the recovery of the colony morphology, the EPS per cell increased and then decreased, with TB-EPS and B-polysaccharides constituting the primary components. The results suggest that colony formation transitioned from adhesion driven to being division driven over time. It is suggested that the homologous EPS released into the ambient environment due to the disaggregation of the colony is a chemical cue that can affect the formation of a colony. This plays an important but largely ignored role in the dynamics of Microcystis and surface blooms.
Between individualization and politicization: young people's interpretation patterns and action orientations in the face of climate change
Authors: Reuter Leonie , Frick Vivian
Published: March 2024

This paper examines the prevailing interpretation patterns and action orientations regarding climate change and climate protection among the young generation (14–22 years) in Germany. Based on a representative survey, we investigate which climate action options are currently favored and widespread among young individuals in Germany, encompassing both private sphere behavior—sustainable consumption—and public sphere behavior—collective climate action and civic engagement. Subsequently, through qualitative interviews, we delve into the shared interpretation patterns that young individuals draw upon to comprehend, evaluate, and guide their actions in climate protection. In this process, an individualizing and a politicizing interpretation pattern are identified and juxtaposed. As a result, both the representative survey and the qualitative analysis underscore a deep-rooted and widespread adoption of the individualizing rationale among young people in interpreting and acting on climate change. We discuss this finding by exploring the discursive origins of the dominant interpretation pattern and by questioning the respective transformative potential of both the individualizing and the politicizing action orientation.
Potentials of arts education initiatives for promoting emotional wellbeing of Chinese university students
Authors: Guo Yanan
Published: March 2024

The present study explores the intricate dynamics influencing the self-efficacy of Chinese university students through the interplay of participation in artistic activities, positive psychological capital, and emotional intelligence. In the context of technological advancements and various challenges post pandemic, this study delves into the multifaceted aspects of university life, where arts education plays a pivotal role in addressing students' emotional needs. By integrating emotional intelligence with self-efficacy, this study underscores the positive impact of artistic engagement on self-efficacy, while emphasizing on the transformative power of these pursuits. Also, this study establishes that the optimism and resilience contribute to this relationship by considering the mediating role of positive psychological capital. The moderating influence of emotional intelligence in the complex dynamics between arts education and positive psychological capital is another concern, thereby emphasizing the nuanced role of emotional intelligence. With a structured set of questions that were administered to 673 participants with 93.61% recovery rate, this study performs the Cronbach's α-test, validation factor, and several related tests in SPSSStatistics 29.0, bootstrap, and AMOS 25.0 software. Current results shows the importance of a holistic approach in Chinese institutions. With a focus on promoting artistic engagement to enhance students' self-efficacy, this study determines the profound impact of arts education on students' overall wellbeing and educational experience. In conclusion, this research highlights the constructive impact of artistic engagement on the self-efficacy of Chinese university students. Chinese institutions should encourage a varied range of artistic engagements as a response to the contemporary challenges confronted by their students.
Histories of violence among clients seeking substance use disorder treatment: a systematic mapping review
Authors: Beeler Sara , Gerrish Olivia , Aldred B. Grantham , Asher BlackDeer Autumn
Published: March 2024

Introduction

There is a limited literature base regarding the intersection of drug and alcohol treatment, violence, and trauma. While research substantiates that exposure to violence and trauma impacts the propensity to misuse substances, the conceptualization in clinical trials and practice has largely been narrow and gendered, referring only to intimate partner or domestic violence. Our systematic mapping review explored a more inclusive and expansive review of survivors of and perpetrators of violence and trauma (e.g., intimate partner violence, sexual assault, stalking, child abuse, political and community violence, criminal violence, micro violence, structural violence, and oppression) to establish: 1) the types of treatment settings included in intervention studies, 2) the common indicators of success or common outcomes recorded, and 3) understanding who is seeking treatment for drug and alcohol use with histories of violence.

Methods

A systematic mapping review was conducted to identify any peer-reviewed articles published from 2011 to 2022. The Web of Science database was searched using a broad set of Boolean search terms related to violence, substance use disorders, and treatment. Over 8,800 records were identified from the systematic review with a total of 48 articles meeting inclusion criteria.

Results

Most studies in this review included populations reporting perpetration of violence (n=23, 48%) versus participants reporting survival of trauma/violence (n=17, 35%). Results also indicated female identifying populations (n=19; 40%) were predominantly served, were treated in the US (n=33; 69%) and seen in an outpatient setting (n=24; 50%). Authors also were attentive to studies that included sexual and gender minorities and discovered only three studies (6%) explicitly acknowledging inclusion of transgender participants or participants in relationship with partners of the same sex; three more studies (6%) were focused on participants with histories of or engaging in sex work.

Discussion

This review outlines treatment and research implications directly situated in the gap of service delivery found in this review. Specifically, the results elucidate the impact on minoritized and excluded identities based on gender, sexual preference, criminal legal status and directions for research and treatment to increase inclusion, representation, and equity across research and treatment settings.
Prevalence of posttraumatic stress disorder and associated factors among displaced people in Africa: a systematic review and meta-analysis
Authors: Andualem Fantahun , Melkam Mamaru , Takelle Girmaw Medfu , Nakie Girum , Tinsae Techilo , Fentahun Setegn , Rtbey Gidey , Begashaw Tesfaye Derbie , Seid Jemal , Tegegn Lidiya Fasil , Gedef Getachew Muluye , Bitew Desalegn Anmut , Godana Tilahun Nega
Published: March 2024

Background

The number of people who have been displaced from their homes due to violence, conflict, and natural disasters. The displaced persons are vulnerable to PTSD; however, being women, individuals with lower socio-economic status and intense exposure to physical assault are more vulnerable. The reviews stated that the pooled prevalence of PTSD among refugees in high-income countries was higher than the general population. However, there has been no review done on PTSD among displaced persons in Africa. Therefore, the aim of this review was to summarise the most recent data evidence on the pooled prevalence of posttraumatic stress disorder and the pooled effect of associated factors on adult displaced people in Africa.

Methods

We used an appropriate guideline for systematic reviews and meta-analyses reports, which is the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). This review protocol was registered in PROSPERO (CRD42023411371). The publications were identified from PubMed/Medline, EMBASE, the Cochrane Library, Scopus databases, and other grey searches of Google Scholar and World Health Organisation (WHO) reports. The data was extracted in Microsoft Excel, and then it will be imported into STATA 11.0 for analysis.

Results

We have included 10 studies conducted in African countries with 5287 study participants. In this meta-analysis, the pooled prevalence of PTSD among displaced people in Africa was 55.64 (95% CI: 42.76–68.41%). Further, in subgroup analysis regarding the study participants, the pooled prevalence of PTSD among internally displaced people and refugees was 56.35% and 54.04%, respectively. Among the associated factors, being female, unemployed, and depression were significantly related to PTSD among displaced people.

Conclusions

In this review, the pooled prevalence of PTSD among displaced people in Africa was high. Demographic characteristics (female, single, and unemployed), substance use disorder, and depression were risk factors for PTSD among displaced people. This finding might help the stakeholders (mental health policy makers, administrators, and mental health professionals) to address the prevention, early screening, and management of PTSD among displaced people and to give attention to more vulnerable bodies.

Systematic review registration

PROSPERO, identifier CRD42023411371.
An exploratory study on the association of multiple metals in serum with preeclampsia
Authors: He Jie , Pu Yudong , Du Yue , Liu Haixia , Wang Xiaoxue , He Shuzhen , Ai Shiwei , Dang Yuhui
Published: March 2024

Background

Individual metal levels are potential risk factors for the development of preeclampsia (PE). However, understanding of relationship between multiple metals and PE remains elusive.

Purpose

The purpose of this study was to explore whether eight metals [zinc (Zn), manganese (Mn), copper (Cu), nickel (Ni), lead (Pb), arsenic (As), cadmium (Cd), and mercury (Hg)] in serum had a certain relationship with PE.

Methods

A study was conducted in Dongguan, China. The concentrations of metals in maternal serum were assessed using inductively coupled plasma mass spectrometry (ICP-MS). Data on various factors were collected through a face-to-face interview and hospital electronic medical records. The unconditional logistic regression model, principal component analysis (PCA) and Bayesian Kernel Machine Regression (BKMR) were applied in our study.

Results

The logistic regression model revealed that the elevated levels of Cu, Pb, and Hg were associated with an increased risk of PE. According to PCA, principal component 1 (PC1) was predominated by Hg, Pb, Mn, Ni, Cu, and As, and PC1 was associated with an increased risk of PE, while PC2 was predominated by Cd and Zn. The results of BKMR indicated a significant positive cumulative effect of serum metals on PE risk, with Ni and Cu exhibiting a significant positive effect. Moreover, BKMR results also revealed the nonlinear effects of Ni and Cd.

Conclusion

The investigation suggests a potential positive cumulative impact of serum metals on the occurrence of PE, with a particular emphasis on Cu as a potential risk factor for the onset and exacerbation of PE. These findings offer valuable insights for guiding future studies on this concern.
Chemical analysis and bioactive compounds from agrifood by-products of peruvian crops
Authors: Inga Marianela , Betalleluz-Pallardel Indira , Puma-Isuiza Gustavo , Cumpa-Arias Leslie , Osorio Coralia , Valdez-Arana Jenny-Del-Carmen , Vargas-De-La-Cruz Celia
Published: March 2024

Many countries face the problem of agroindustrial waste elimination, which is linked to environmental damage caused by improper waste management. These wastes contain bioactive compounds often discarded due to a lack of knowledge. The objective of this study was to analyze five types of agroindustrial waste from Peru, including avocado (Persea americana) seeds, lucuma (Pouteria lucuma) seeds, grape (Vitis vinifera) pomace, mango (Mangifera indica) peel, and cocoa (Theobroma cacao) pods. This study includes proximal analysis, determination of total phenolic compound content, analysis of phenolic compounds by UHPLC– Q/Orbitrap/ESIMS/MS (Ultrahigh-performance liquid chromatography-electrospray ionization Q-Orbitrap mass spectrometry), and the evaluation of antioxidant, antimicrobial, and antifungal activities of different samples. The results showed that lucuma seeds had the highest protein content of 6.59 g/100 g DW. Mango peels showed the highest fat content of 4.89 g/100 g DW, cocoa pods had the highest crude fiber content of 26.91 g/100 g DW, and cocoa pods exhibited the highest ash content of 7.14 g/100 g DW. The highest carbohydrate content was found in avocado seeds at 85.85 g/100 g DW. Mango peels (56.66 mg GAE/g DW), avocado seeds (56.35 mg GAE/g DW), and cocoa pods (51.52 mg GAE/g DW) extracts showed the highest phenolic compound contents. Mango peel extract exhibited the highest antioxidant activities evaluated by the ABTS (995.01 μmol TEAC/g DW), DPPH (953.54 μmol TEAC/g DW), and FRAP (1661.13 μmol TEAC/g DW) assays. Extracts from cocoa pods, grape pomace, and lucuma seeds showed inhibitory activity on the growth of Staphylococcus aureus and Escherichia coli. In contrast, the extract of lucuma seeds was the only one that showed antifungal activity over Penicillium digitatum. As a result, several phenolic compounds were detected in each, indicating their potential use in developing functional foods and food preservation, among other applications.