bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effects of a Standardized Cognitive-Behavioural Therapy and an Additional Mindfulness-Based Training on Interoceptive Abilities in a Depressed Cohort
Authors: Karanassios Georgios , Schultchen Dana , Möhrle Matthias , Berberich Götz , Pollatos Olga
Published: October 2021

Background: Interoceptive accuracy and sensibility are decreased in depressive samples. However, different studies showed that cognitive-behavioural therapy (CBT) and mindfulness interventions are promising approaches to improve interoceptive abilities. Based on these findings, the study aims to investigate the pre–post effect of CBT in a depressive sample. Additionally, we examined the effect of mindfulness-based stress reduction (MBSR) training in the context of CBT. Methods: Sixty depressive patients were investigated over four weeks, with two conditions—CBT vs. CBT + MBSR. Further, the changes in interoceptive abilities (interoceptive accuracy and sensibility) of the depressive patients were compared to baseline data of healthy controls. Results: The depressive patients showed significantly higher levels of depression and lower mindfulness and interoceptive abilities than healthy controls. The depressive sample showed a significant decrease in depressive symptoms and increased mindfulness and interoceptive abilities after CBT. Lastly, depressive patients of the CBT + MBSR condition did not differ from those who only received CBT in the levels of depression, mindfulness or interoceptive abilities over the time course. Discussion: This study demonstrates a positive effect of CBT on interoceptive abilities in a depressive sample. It is shown that the depressive sample did not profit from additional mindfulness training. It can be concluded that CBT is an efficient treatment, resulting in increased interoceptive abilities. Unexpectedly, the combination of CBT and MBSR has no additional effect on these changes. Future studies should investigate the effect of MBSR as a stand-alone therapy.
Light Transmitting Concrete: A Review
Authors: Luhar Ismail , Luhar Salmabanu , Savva Pericles , Theodosiou Antreas , Petrou Michael , Nicolaides Demetris
Published: October 2021

Recently, research attention has been drawn to the application of novel, unique, and innovative types of construction materials to fulfil diverse objectives associated with the ground-breaking concept of “Greener Architecture”, in order to improve the overall economic value and quality of construction. Among these revolutionary structural building materials is light-transmitting concrete, also referred to as translucent or transparent concrete. This material is based on the concept of nano-optics, which allows exterior light to transmit through internal spaces in which light elements, namely optical fibres, are incorporated during the material’s manufacture. The current review assesses earlier studies of translucent concrete, focusing on its applications, and the appropriate ratio and arrangement pattern of optical fibres. This study also investigated the light-transmitting, mechanical, thermal, and energy-saving properties of translucent concrete by analysing research conducted during the past decade. However, numerous material restrictions and research gaps were found in the earlier literature on this concrete. The principal restrictions relate to the material’s low material strength and the identification of the optimum ratio of fibres. The main gaps identified among the reviewed research investigations relate to tests aiming to identify the influence of dissimilar ratios of optical fibres on the material’s strength and energy-saving properties. In the current review, we also identify and recommend future areas of research, and provide suggestions to address the existing research gaps. Finally, we review the types of translucent materials, their properties, and their advantages and disadvantages, and provide illustrations and value-added applications. The aim is to promote translucent concrete as an attractive, promising, and innovative building material for the construction industry.
Effect of Amount of Fibre and Damage Level on Service Life of SFR Recycled Concrete in Aggressive Environment
Authors: Lehner Petr , Horňáková Marie
Published: October 2021

The paper presents a numerical calculation of the service life of concrete structures considering the effect of chlorides in the case of the material properties of structural lightweight waste aggregate concrete. Different amounts of fibres (0.0%, 1.0%, and 1.5%) and different values of compressive preloading (0%, 50%, and 100% of the ultimate strength capacity-USC) were considered. The subject of the research was the comparison of the influence of the constant diffusion coefficient and the time-dependent diffusion coefficient regarding the service life of the selected structure. Nine groups of material characteristics in combination with two numerical models are compared. A time-dependent diffusion coefficient and maturation coefficient, which were determined based on long-term monitoring (up to 461 days), were accepted for the numerical modelling. Thanks to time-dependent parameters, it is possible to observe the results of the theoretical service life of the structure and the influence of the mentioned factors. The analysed structure can be considered as the upper layer of an industrial floor in a chemical plant. It is important to determine the theoretical service life at which the structure shall be inspected or replaced. The results, in general, show that a higher amount of fibres reduces the service life as well as the preloading of the structure. An exception was a mixture with 1% of fibre loaded to 50% USC, which shows a lower diffusion coefficient than the specimens without preloading.
Detection of Pancreatic Ductal Adenocarcinoma by Ex Vivo Magnetic Levitation of Plasma Protein-Coated Nanoparticles
Authors: Digiacomo Luca , Quagliarini Erica , La Vaccara Vincenzo , Coppola Alessandro , Coppola Roberto , Caputo Damiano , Amenitsch Heinz , Sartori Barbara , Caracciolo Giulio , Pozzi Daniela
Published: October 2021

Pancreatic Ductal Adeno Carcinoma (PDAC) is one of the most lethal malignancies worldwide, and the development of sensitive and specific technologies for its early diagnosis is vital to reduce morbidity and mortality rates. In this proof-of-concept study, we demonstrate the diagnostic ability of magnetic levitation (MagLev) to detect PDAC by using levitation of graphene oxide (GO) nanoparticles (NPs) decorated by a biomolecular corona of human plasma proteins collected from PDAC and non-oncological patients (NOP). Levitation profiles of corona-coated GO NPs injected in a MagLev device filled with a paramagnetic solution of dysprosium(III) nitrate hydrate in water enables to distinguish PDAC patients from NOP with 80% specificity, 100% sensitivity, and global classification accuracy of 90%. Our findings indicate that Maglev could be a robust and instrumental tool for the early detection of PDAC and other cancers.
Inflammatory Mediators and Gut Microbial Toxins Drive Colon Tumorigenesis by IL-23 Dependent Mechanism
Authors: Panneerselvam Janani , Madka Venkateshwar , Rai Rajani , Morris Katherine T. , Houchen Courtney W. , Chandrakesan Parthasarathy , Rao Chinthalapally V.
Published: October 2021

Obesity-associated chronic inflammation predisposes colon cancer risk development. Interleukin-23 (IL-23) is a potential inflammatory mediator linking obesity to chronic colonic inflammation, altered gut microbiome, and colon carcinogenesis. We aimed to elucidate the role of pro-inflammatory eicosanoids and gut bacterial toxins in priming dendritic cells and macrophages for IL-23 secretion to promote colon tumor progression. To investigate the association of IL-23 with obesity and colon tumorigenesis, we utilized TCGA data set and colonic tumors from humans and preclinical models. To understand IL-23 production by inflammatory mediators and gut microbial toxins, we performed several in vitro mechanistic studies to mimic the tumor microenvironment. Colonic tumors were utilized to perform the ex vivo experiments. Our findings showed that IL-23 is elevated in obese individuals, colonic tumors and correlated with reduced disease-free survival. In vitro studies showed that IL-23 treatment increased the colon tumor cell self-renewal, migration, and invasion while disrupting epithelial barrier permeability. Co-culture experiments of educated dendritic cells/macrophages with colon cancer cells significantly increased the tumor aggression by increasing the secretory levels of IL-23, and these observations are further supported by ex vivo rat colonic tumor organotypic experiments. Our results demonstrate gut microbe toxins and eicosanoids facilitate IL-23 production, which plays an important role in obesity-associated colonic tumor progression. This newly identified nexus represents a potential target for the prevention and treatment of obesity-associated colon cancer.
Intra-Tumour Genetic Heterogeneity and Prognosis in High-Risk Neuroblastoma
Authors: López-Carrasco Amparo , Berbegall Ana P. , Martín-Vañó Susana , Blanquer-Maceiras Maite , Castel Victoria , Navarro Samuel , Noguera Rosa
Published: October 2021

Spatial ITH is defined by genomic and biological variations within a tumour acquired by tumour cell evolution under diverse microenvironments, and its role in NB patient prognosis is understudied. In this work, we applied pangenomic techniques to detect chromosomal aberrations in at least two different areas of each tumour and/or in simultaneously obtained solid and liquid biopsies, detecting ITH in the genomic profile of almost 40% of HR-NB. ITH was better detected when comparing one or more tumour pieces and liquid biopsy (50%) than between different tumour pieces (21%). Interestingly, we found that patients with ITH analysed by pangenomic techniques had a significantly better survival rate that those with non-heterogeneous tumours, especially in cases without MYCN amplification. Moreover, all patients in the studied cohort with high ITH (defined as 50% or more genomic aberration differences between areas of a tumour or simultaneously obtained samples) survived after 48 months. These results clearly support analysing at least two solid tumour areas (separately or mixed) and liquid samples to provide more accurate genomic diagnosis, prognosis and therapy options in HR-NB.
Co-Expression with Membrane CMTM6/4 on Tumor Epithelium Enhances the Prediction Value of PD-L1 on Anti-PD-1/L1 Therapeutic Efficacy in Gastric Adenocarcinoma
Authors: Wang Ziqi , Peng Zhi , Liu Qiyao , Guo Zixia , Menatola Merey , Su Jing , Li Ting , Ge Qing , Wang Pingzhang , Shen Lin , Jin Rong
Published: October 2021

Anti-PD-1/L1 immunotherapy has been intensively used in heavily treated population with advanced gastric adenocarcinoma. However, the immunotherapeutic efficacy is low even in PD-L1 positive patients. We aimed to establish a new strategy based on the co-expression of CMTM6/4 and PD-L1 for patient stratification before immunotherapy. By analyzing the data obtained from TCGA and single-cell RNA sequencing at the mRNA level, and 6-color multiplex immunofluorescence staining of tumor tissues in tissue array and 48-case pre-immunotherapy patients at the protein level, we found that CMTM6/4 and PD-L1 co-expressed in both epithelial and mesenchymal regions of gastric adenocarcinoma. The tumor tissues had higher levels of CMTM6/4 expression than their adjacent ones. A positive correlation was found between the expression of CMTM6/4 and the expression of PD-L1 in tumor epithelium. Epithelial co-expression of CMTM6/4 and PD-L1 in gastric tumor region was associated with shorter overall survival but better short-term response to anti-PD-1/L1 immunotherapy. Thus, we developed a predictive model and three pathological patterns based on the membrane co-expression of CMTM6/4 and PD-L1 in tumor epithelial cells for pre-immunotherapy patient screening in gastric adenocarcinoma.
Metabolomic Profiling in Renal Cell Carcinoma Patients: News and Views
Authors: Aurilio Gaetano , Santoni Matteo , Massari Francesco , Cimadamore Alessia , Rizzo Alessandro , Mollica Veronica , Verri Elena , Battelli Nicola , Montironi Rodolfo
Published: October 2021

Background: We address novelty regarding metabolomic profiling in renal cell carcinoma (RCC) patients, in an attempt to postulate potential treatment strategies. Methods: A large-scale literature search in existing scientific websites focusing on the keywords “renal cell carcinoma”, “clear cell histology”, “papillary histology”, “metabolomic profiling”, and “therapeutics” was performed. Results: The PI3K/Akt signaling pathway is key in clear cell RCC metabolism and accordingly several drugs are presently available for routine use in clinical practice. Along this line, new treatment combinations against PI3K/Akt family members are currently under clinical investigation. On the other hand, new developed targets such as c-Met tyrosine kinase domain, glutathione (GSH) metabolism, and histone deacetylases enzymes (HDAC), as well as therapeutic strategies targeting them are currently being tested in clinical trials and here discussed. Conclusions: In RCC patients, the PI3K/Akt signaling is still the most effective targetable pathway. Targeting other metabolic pathways such as c-Met, GSH, and HDAC appears to be a promising approach and deserve further insights.
Novel Balance Mechanism Participates in Stem Cell Therapy to Alleviate Neuropathology and Cognitive Impairment in Animal Models with Alzheimer’s Disease
Authors: Qin Chuan , Li Yongning , Wang Kewei
Published: October 2021

Stem cell therapy improves memory loss and cognitive deficits in animal models with Alzheimer’s disease. The underlying mechanism remains to be determined, but it may involve the interaction of stem cells with hippocampal cells. The transplantation of stem cells alters the pathological state and establishes a novel balance based on multiple signaling pathways. The new balance mechanism is regulated by various autocrine and paracrine cytokines, including signal molecules that target (a) cell growth and death. Stem cell treatment stimulates neurogenesis and inhibits apoptosis, which is regulated by the crosstalk between apoptosis and autophagy—(b) Aβ and tau pathology. Aberrant Aβ plaques and neurofibrillary tau tangles are mitigated subsequent to stem cell intervention—(c) inflammation. Neuroinflammation in the lesion is relieved, which may be related to the microglial M1/M2 polarization—(d) immunoregulation. The transplanted stem cells modulate immune cells and shape the pathophysiological roles of immune-related genes such as TREM2, CR1, and CD33—(e) synaptogenesis. The functional reconstruction of synaptic connections can be promoted by stem cell therapy through multi-level signaling, such as autophagy, microglial activity, and remyelination. The regulation of new balance mechanism provides perspective and challenge for the treatment of Alzheimer’s disease.
Integrative Analysis to Identify Genes Associated with Stemness and Immune Infiltration in Glioblastoma
Authors: Warrier Neerada Meenakshi , Agarwal Prasoon , Kumar Praveen
Published: October 2021

It is imperative to identify the mechanisms that confer stemness to the cancer cells for more effective targeting. Moreover, there are not many studies on the link between stemness characteristics and the immune response in tumours. Therefore, in the current study involving GBM, we started with the study of BIRC5 (one of the rare genes differentially expressed in normal and cancer cells) and CXCR4 (gene involved in the survival and proliferation of CSCs). Together, these genes have not been systematically explored. We used a set of 27 promoter methylated regions in GBM. Our analysis showed that four genes corresponding to these regions, namely EOMES, BDNF, HLA-A, and PECAM1, were involved with BIRC5 and CXCR4. Interestingly, we found EOMES to be very significantly involved in stemness and immunology and it was positively correlated to CXCR4. Additionally, BDNF, which was significant in methylation, was negatively correlated to BIRC5.
What Do We Know about Pruritus in Very Young Infants? A Literature Review
Authors: Le Pors Camille , Talagas Matthieu , Abasq-Thomas Claire , Henry Séverine , Misery Laurent , Roué Jean-Michel
Published: October 2021

In infants, pruritus is frequently considered as absent because they do not scratch themselves. Because pruritus could induce severe adverse effects in this vulnerable population, we aimed to review existing evidence on the ability of young infants to experience itch and on how to assess itch-related discomfort in this population. A literature review was performed (Pubmed, Google Scholar). Neurological itch pathways are well described. Skin development starts early during gestation. At 34 weeks of gestation, skin is almost complete while skin adaptations occur after birth. Newborn skin is neurologically functional, including the ability for young infants to feel pain. Similarities and interactions between pain and pruritus support the hypothesis that infants could feel pruritus. However, the existence of pruritus in infants has never been evidenced. Many itchy conditions can affect them, suggesting non-negligible prevalence of infant pruritus among which atopic dermatitis (AD) is the most studied disease. Studies reported a negative impact of AD on children and their families. There is no existing validated method to assess pruritus in infants, although they may feel pruritus and chronic pruritus can lead to serious adverse effects. To appropriately diagnose pruritus appears of great interest among young infants. Development of a method is required to this aim.
Chorioamnionitis, Inflammation and Neonatal Apnea: Effects on Preterm Neonatal Brainstem and on Peripheral Airways: Chorioamnionitis and Neonatal Respiratory Functions
Authors: Vitaliti Giovanna , Falsaperla Raffaele
Published: October 2021

Background: The present manuscript aims to be a narrative review evaluating the association between inflammation in chorioamnionitis and damage on respiratory centers, peripheral airways, and lungs, explaining the pathways responsible for apnea in preterm babies born by delivery after chorioamnionitis. Methods: A combination of keywords and MESH words was used, including: “inflammation”, “chorioamnionitis”, “brainstem”, “cytokines storm”, “preterm birth”, “neonatal apnea”, and “apnea physiopathology”. All identified papers were screened for title and abstracts by the two authors to verify whether they met the proper criteria to write the topic. Results: Chorioamnionitis is usually associated with Fetal Inflammatory Response Syndrome (FIRS), resulting in injury of brain and lungs. Literature data have shown that infections causing chorioamnionitis are mostly associated with inflammation and consequent hypoxia-mediated brain injury. Moreover, inflammation and infection induce apneic episodes in neonates, as well as in animal samples. Chorioamnionitis-induced inflammation favors the systemic secretion of pro-inflammatory cytokines that are involved in abnormal development of the respiratory centers in the brainstem and in alterations of peripheral airways and lungs. Conclusions: Preterm birth shows a suboptimal development of the brainstem and abnormalities and altered development of peripheral airways and lungs. These alterations are responsible for reduced respiratory control and apnea. To date, mostly animal studies have been published. Therefore, more clinical studies on the role of chorioamninitis-induced inflammation on prematurity and neonatal apnea are necessary.
Oxygen Barrier Performance of Poly(vinyl alcohol) Coating Films with Different Induced Crystallinity and Model Predictions
Authors: Idris Alamin , Muntean Adrian , Mesic Beko , Lestelius Magnus , Javed Asif
Published: October 2021

The presence of the crystalline regions in poly(vinyl alcohol) coating films acts as barrier clusters forcing the gas molecules to diffuse in a longer pathway in the amorphous region of the polymer, where diffusivity and solubility are promoted in comparison. Evaluating the influence of crystalline regions on the oxygen barrier property of a semi-crystalline polymer is thus essential to prepare better coating films. Poly(vinyl alcohol) coating films with varying induced crystallinity were prepared on a polyethylene terephthalate (PET) substrate by drying at different annealing temperatures for 10 min. The coating films were first delaminated from the PET substrate and then characterized using Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC), and X-ray diffraction (XRD) techniques to determine and confirm the induced percentage of crystallinity. The barrier performance of the coating films, i.e., the oxygen transmission rate (OTR), was measured at room temperature. Results showed a decrease in the OTR values of poly(vinyl alcohol) film with an increase in the degree of crystallinity of the polymer matrix. Tortuosity-based models, i.e., modified Nielsen models, were adopted to predict the barrier property of the semi-crystalline PVOH film with uniform or randomly distributed crystallites. A modified Nielsen model for orderly distributed crystallites with an aspect ratio of 3.4 and for randomly distributed crystallites with an aspect ratio of 10.4 resulted in a good correlation with the experimental observation. For the randomly distributed crystallites, lower absolute average relative errors of 4.66, 4.45, and 5.79% were observed as compared to orderly distributed crystallites when the degree of crystallinity was obtained using FTIR, DSC, and XRD data, respectively.
Green Approach in Synthesis of Bio-Inspired Materials
Authors: Stanković Anamarija , Medvidović-Kosanović Martina , Kontrec Jasminka , Džakula Branka Njegić
Published: October 2021

In this Special Issue, we focus on biomineralization/pathological biomineralization systems and the synthesis of bioinspired materials [...]
Thermal Deformation Behavior of Ti-6Mo-5V-3Al-2Fe Alloy
Authors: Han Lin , Zhang Haoyu , Cheng Jun , Zhou Ge , Wang Chuan , Chen Lijia
Published: October 2021

The Gleeble-3800 thermal simulation machine was used to perform hot compression experiments on a new type of β alloy, Ti-6Mo-5V-3Al-2Fe (wt.%), at temperatures of 700–900 °C, strain rates of 5 × 10−1 to 5 × 10−4 s−1, and total strain of 0.7. Transmission and EBSD techniques were used to observe the microstructure. The results show that the deformation activation energy of the alloy was 356.719 KJ/mol, and dynamic recrystallization occurred during the hot deformation. The higher the deformation temperature was, the more obvious the dislocations that occurred and the more sufficient the dynamic recrystallization that occurred, but the effect of strain rate was the opposite. When the deformation temperature was higher than the phase transition point, the recrystallized grains clearly grew up. The calculated strain rate sensitivity index of the alloy was 0.14–0.29. The constitutive equation of hot deformation of Ti-6Mo-5V-3Al-2Fe alloy was established by using the Arrhenius hyperbolic sine equation. The dynamic DMM hot working diagram with the strain of 0.7 was constructed. The relatively good hot working area of the alloy was determined to be the deformation temperature of 700–720 °C and 0.0041–0.0005 s−1.
Total Colectomy as a Part of Ultra-Radical Surgery for Ovarian Cancer—Short- and Long-Term Outcomes
Authors: Szubert Sebastian , Skowyra Artur , Wójtowicz Andrzej , Pawlowicz Pawel , Szczepkowski Marek , Nowakowski Blazej , Wicherek Lukasz
Published: October 2021

(1) Background: The aim of this study was to assess the outcomes for patients who underwent total colectomy (TC) as a part of surgery for ovarian cancer (OC). (2) Methods: We performed a retrospective analysis of 1636 OC patients. Residual disease (RD) was reported using Sugarbaker’s completeness of cytoreduction score. (3) Results: Forty-two patients underwent TC during primary debulking surgery (PDS), and four and ten patients underwent TC during the interval debulking surgery (IDS) and secondary cytoreduction, respectively. The median overall survival (mOS) in OC patients following the PDS was 45.1 months in those with CC-0 (21%) resection, 11.1 months in those with CC-1 (45%) resection and 20.0 months in those with CC-2 (33%) resection (p = 0.28). Severe adverse events were reported in 18 patients (43%). In the IDS group, two patients survived more than 2 years after IDS and one patient died after 28.6 months. In the recurrent OC group, the mOS was 6.9 months. Patient age above 65 years was associated with a shortened overall survival (OS) and the presence of adverse events. (4) Conclusions: TC as a part of ultra-radical surgery for advanced OC results in high rates of optimal debulking. However, survival benefits were observed only in patients with no macroscopic disease.
Therapeutic Effect of Polidocanol Sclerotherapy on Oral Vascular Malformations
Authors: Fukuzawa Satoshi , Yamagata Kenji , Okubo-Sato Makiko , Terada Kazuhiro , Uchida Fumihiko , Ishibashi-Kanno Naomi , Bukawa Hiroki
Published: October 2021

Various treatments for oral vascular malformation (VM) have been reported. Polidocanol and absolute ethanol have also been reported for sclerotherapy. However, there are still few reports on the therapeutic effect and dosage of polidocanol sclerotherapy. Therefore, we examined its therapeutic effects on oral VM. There were 17 sites of VMs, with nine patients diagnosed with oral VM at the Department of Dental and Oral Surgery, Tsukuba University Hospital. The medical records were retrospectively investigated to determine the site, hemangioma volume, polidocanol injection volume, and therapeutic effect. The volume of hemangiomas was calculated using magnetic resonance images. Based on the site, oral VMs were observed in the tongue, buccal mucosa, lips, and oral floor in eight, three, five, and one patients, respectively. The average size of the site was 3071 mm3. The average injection dose of polidocanol at one site was 2.86 mL, the average number of administrations was 1.6, and the response rate was 88.2%. No adverse events were observed. The median numerical rating scale scores were 2/10 (0–6/10) and 0/10 (0–1/10) the day after surgery and 1 week after surgery, respectively. Univariate regression analysis of the total dose in successful cases provided the following formula: 1.3 + 0.00025 × volume (mm3) (mg). Polidocanol sclerotherapy is an effective treatment method for oral VM.
The Learning of E-Sustainability Competences: A Comparative Study between Future Early Childhood and Primary School Teachers
Authors: Urrea-Solano Mayra , Hernández-Amorós María J. , Merma-Molina Gladys , Baena-Morales Salvador
Published: October 2021

Digital technologies play a key role in the fulfilment of the 2030 Agenda. However, their contribution to this goal depends on the digital culture of society. In this context, future teachers’ knowledge of e-sustainability is of paramount importance, as the responsible and sustainable behaviour of future generations largely depends on their skills in this area. Therefore, this study aimed to identify the existence of possible differences in digital competences in sustainability among trainee teachers. The study involved the participation of 348 students in the 2nd year of their Bachelor’s Degrees in Early Childhood and Primary Education at the University of Alicante (Alicante, Spain), who filled out a questionnaire on this topic. The SPSS v. 25 statistical programme, with which a comparative analysis was carried out, was used to process the data. On the basis of the results, the students of the Bachelor’s Degree in Early Childhood Education generally presented a higher level of e-sustainable competences, especially with regard to general competences and the economic dimension of digital sustainability. Despite this, and given the small size of the differences, we conclude that there is a need to design didactic proposals to favour the acquisition of these competences among future teachers at both stages.
Existing and Emerging Students’ Alternative Ideas on Geodynamic Phenomena: Development, Controlling Factors, Characteristics
Authors: Bakopoulou Athanasia , Antonarakou Assimina , Zambetakis-Lekkas Alexandra
Published: October 2021

This paper studies Greek junior high school students’ alternative ideas, both initial and synthetic, on geodynamic phenomena. It comments in detail on students’ concepts on Earth structure, earthquake occurrence, volcano formation, and relief change. Additionally, it attempts to trace and interpret how and why these ideas form (concept development), presenting that initial and synthetic ones are indissolubly attached and utterly directed by environmental interaction. Data analysis verifies that curriculum inadequacy and false scientific terminology in textbooks enforce the generation of alternative ideas. New synthetic alternative ideas on geodynamic phenomena are presented which are mainly characterized by intermittent and fragmentary perspective. Furthermore, the characteristics of both initial and synthetic alternative ideas are outlined, giving emphasis on the facts that students represent geodynamic phenomena as instantaneous events and that they are able to describe the repeatability of the phenomena, but they show difficulty in capturing their continuity. Finally, more factors that control alternative idea development on geodynamic phenomena are highlighted—such as (i) lack of continuous thinking, (ii) distribution, intensity and frequency of geodynamic phenomena, and (iii) current affairs (i.e., pollution, technology evolution, human intervention)—hoping that their revelation will lead to alternative ideas’ decomposition and thus to pure scientific knowledge.
Context-Aware Content Selection and Message Generation for Collective Perception Services
Authors: Chtourou Ameni , Merdrignac Pierre , Shagdar Oyunchimeg
Published: October 2021

Collective perception messages (CPMs) inform neighbors about detected objects and their current status. Although CPMs can greatly contribute to improving vehicle perception, their performance will degrade if the wireless channel is saturated and/or vehicles must process excessively high amount of data to build such a perception. Channel saturation and processing overhead can be induced by transmissions of CPMs if vehicles and roadside infrastructure transmit CPMs too often with too much redundant information. Indeed, according to the current draft standard, the CPM generation frequency at individual vehicles and infrastructure can be as high as 10 Hz and depending on the perception capability, individual CPMs can be quite heavy (around 1500 Bytes), containing redundant information. It is hence important to integrate techniques that reduce information redundancy while providing sufficient perception with optimal resource use. In this paper, we propose context-aware communication schemes that control CPM content selection and transmission. Two types of contexts, particularly radio resource use (channel busy ratio: CBR) and infrastructure availability, have been considered in the proposed schemes. Using a network simulator, we evaluated the performances of the proposed schemes in terms of CBR, packet delivery ratio and awareness ratio. The simulation results show that the scheme that takes into account both resource use and infrastructure availability offers the best performance on all the above metrics.
On Optimizing a Multi-Mode Last-Mile Parcel Delivery System with Vans, Truck and Drone
Authors: Wang Chuan , Lan Hongjie , Saldanha-da-Gama Francisco , Chen Youhua
Published: October 2021

This work focuses on the optimization of a last-mile delivery system with multiple transportation modes. In this scenario, parcels need to be delivered to each customer point. The major feature of the problem is the combination of a fleet of road vehicles (vans) with a drone. Each van visits a subset of demand nodes to be determined according to the route of the van. The drone serves the customers not served by vans. At the same time, considering the safety, policy and terrain as well as the need to replace the battery, the drone needs to be transported by truck to the identified station along with the parcel. From each such station, the drone serves a subset of customers according to a direct assignment pattern, i.e., every time the drone is launched, it serves one demand node and returns to the station to collect another parcel. Similarly, the truck is used to transport the drone and cargo between stations. This is somewhat different from the research of other scholars. In terms of the joint distribution of the drone and road vehicle, most scholars will choose the combination of two transportation tools, while we use three. The drone and vans are responsible for distribution services, and the trucks are responsible for transporting the goods and drone to the station. The goal is to optimize the total delivery cost which includes the transportation costs for the vans and the delivery cost for the drone. A fixed cost is also considered for each drone parking site corresponding to the cost of positioning the drone and using the drone station. A discrete optimization model is presented for the problem in addition to a two-phase heuristic algorithm. The results of a series of computational tests performed to assess the applicability of the model and the efficiency of the heuristic are reported. The results obtained show that nearly 10% of the cost can be saved by combining the traditional delivery mode with the use of a drone and drone stations.
Step Coverage and Dry Etching Process Improvement of Amorphous Carbon Hard Mask
Authors: Jiang Zheng , Zhu Hao , Sun Qingqing , Zhang Davidwei
Published: October 2021

Amorphous carbon hard mask (ACHM) films have been widely applied as protective components and hard etching masks in lithography and dry etching processes. The capability of lithography is directly dependent on the step coverage (SC) of the ACHM. Poor SC may impact the protection of device patterns during the etching process and lead to overlay marks occurring in lithography. In this work, the ACHM film processing process is engineered and optimized towards better SC through the comparative study of the C2H2 and C3H6 precursors at different temperatures. Furthermore, a process parameter design of experiment (DOE), with C2H2 as a precursor to optimize the dry etching rate, is proposed. The results of the experiment show that the dry etching performance is enhanced by higher power, temperature and C2H2 flow, and a smaller gap, lower pressure and lower carrier gas flow. A selective etching ratio of SiO2 and SiN, with an improved process window, is obtained. ACHM film elimination process is also validated by characterizing the surface roughness. The demonstrated results can be instructive in terms of the optimization of etching process in future semiconductor manufacturing.
Time-Lag Selection for Time-Series Forecasting Using Neural Network and Heuristic Algorithm
Authors: Surakhi Ola , Zaidan Martha A. , Fung Pak Lun , Hossein Motlagh Naser , Serhan Sami , AlKhanafseh Mohammad , Ghoniem Rania M. , Hussein Tareq
Published: October 2021

The time-series forecasting is a vital area that motivates continuous investigate areas of intrigued for different applications. A critical step for the time-series forecasting is the right determination of the number of past observations (lags). This paper investigates the forecasting accuracy based on the selection of an appropriate time-lag value by applying a comparative study between three methods. These methods include a statistical approach using auto correlation function, a well-known machine learning technique namely Long Short-Term Memory (LSTM) along with a heuristic algorithm to optimize the choosing of time-lag value, and a parallel implementation of LSTM that dynamically choose the best prediction based on the optimal time-lag value. The methods were applied to an experimental data set, which consists of five meteorological parameters and aerosol particle number concentration. The performance metrics were: Mean Absolute Error (MAE), Root Mean Square Error (RMSE), Mean Absolute Percentage Error (MAPE) and R-squared. The investigation demonstrated that the proposed LSTM model with heuristic algorithm is the superior method in identifying the best time-lag value.
Trends in Coffee and Tea Consumption during the COVID-19 Pandemic
Authors: Castellana Fabio , De Nucci Sara , De Pergola Giovanni , Di Chito Martina , Lisco Giuseppe , Triggiani Vincenzo , Sardone Rodolfo , Zupo Roberta
Published: October 2021

Over the last two years, many countries have enforced confinement to limit both the spread of COVID-19 and the demand for medical care. Confinement has resulted in a disruption of work routines, boredom, depression, and changes in eating habits, among them consumption of coffee and tea. Following six databases, we examined articles tracking consumption of these beverages. Out of 472 articles, including 23 beverage entries, 13 matched our criteria. While no clear trend in coffee consumption during the coronavirus pandemic emerged (7 of 13 studies indicated an increase, accounting for 53.8%), tea consumption clearly increased (70% versus 30%). Considering the global health emergency continuum, more research is needed to better understand the paths underlying food choices and the ways those changes may influence health outcomes, including those related to COVID-19 disease.
More Than a Moggy; A Population Genetics Analysis of the United Kingdom’s Non-Pedigree Cats
Authors: Irving McGrath Jennifer , Zhang Wengang , Hollar Regina , Collings Alison , Powell Roger , Foale Rob D. , Thurley Nicola , Brockman Jeffrey A. , Mellanby Richard J. , Gunn-Moore Danièlle A. , Schoenebeck Jeffrey J.
Published: October 2021

The domestic cat is one of the most popular pets in the world. It is estimated that 89–92% of domestic cats in the UK are non-pedigree Domestic shorthair (DSH), Domestic longhair (DLH), or Domestic semi-longhair cats (DSLH). Despite their popularity, little is known of the UK non-pedigree cats’ population structure and breeding dynamics. Using a custom designed single nucleotide variant (SNV) array, this study investigated the population genetics of 1344 UK cats. Principal components analysis (PCA) and fastSTRUCTURE analysis verified that the UK’s DSH, DLH, and DSLH cats are random-bred, rather than admixed, mix breed, or crossbred. In contrast to pedigree cats, the linkage disequilibrium of these random-bred cats was least extensive and decayed rapidly. Homozygosity by descent (HBD) analysis showed the majority of non-pedigree cats had proportionally less of their genome in HBD segments compared to pedigree cats, and that these segments were older. Together, these findings suggest that the DSH, DLH, and DSLH cats should be considered as a population of random-bred cats rather than a crossbred or pedigree-admixed cat. Unexpectedly, 19% of random-bred cat genomes displayed a higher proportion of HBD segments associated with more recent inbreeding events. Therefore, while non-pedigree cats as a whole are genetically diverse, they are not impervious to inbreeding and its health risks.
Detection of Spinal Muscular Atrophy Patients Using Dried Saliva Spots
Authors: Wijaya Yogik Onky Silvana , Nishio Hisahide , Niba Emma Tabe Eko , Okamoto Kentaro , Shintaku Haruo , Takeshima Yasuhiro , Saito Toshio , Shinohara Masakazu , Awano Hiroyuki
Published: October 2021

Spinal muscular atrophy (SMA) is a lower motor neuron disease, once considered incurable. The main symptoms are muscle weakness and muscular atrophy. More than 90% of cases of SMA are caused by homozygous deletion of survival motor neuron 1 (SMN1). Emerging treatments, such as splicing modulation of SMN2 and SMN gene replacement therapy, have improved the prognoses and motor functions of patients. However, confirmed diagnosis by SMN1 testing is often delayed, suggesting the presence of diagnosis-delayed or undiagnosed cases. To enable patients to access the right treatments, a screening system for SMA is essential. Even so, the current newborn screening system using dried blood spots is still invasive and cumbersome. Here, we developed a completely non-invasive screening system using dried saliva spots (DSS) as an alternative DNA source to detect SMN1 deletion. In this study, 60 DSS (40 SMA patients and 20 controls) were tested. The combination of modified competitive oligonucleotide priming-polymerase chain reaction and melting peak analysis clearly distinguished DSS samples with and without SMN1. In conclusion, these results suggest that our system with DSS is applicable to SMA patient detection in the real world.
Association of CYP26C1 Promoter Hypomethylation with Small Vessel Occlusion in Korean Subjects
Authors: Lee Eun-Ji , Kim Myung-Sunny , Yim Nam-Hui , Cha Min Ho
Published: October 2021

The risk factors for stroke, a fatal disease, include type two diabetes, hypertension, and genetic influences. Small vessel occlusion (SVO) can be affected by epigenetic alterations, but an association between SVO and the methylation of cytochrome P450 family 26 subfamily C member 1 (CYP26C1) has not been identified. In this study, we measured the level of DNA methylation in the CYP26C1 promoter and the 5′ untranslated region of 115 normal subjects and 56 patients with SVO in Korea. The DNA methylation level of each subject was measured by bisulfite amplicon sequencing, and statistical analysis was performed using the general linear model or Pearson’s correlation. The average level of DNA methylation was markedly lower in patients with SVO than in normal subjects (20.4% vs. 17.5%). We found that the methylation of CYP26C1 has a significant positive correlation with blood parameters including white blood cells, hematocrit, lactate dehydrogenase, and Na+ in subjects with SVO. We predicted that binding of RXR-α and RAR-β might be affected by CYP26C1 methylation at CpG sites −246–237 and −294–285. These findings suggest that CYP26C1 methylation in the promoter region may be a predictor of SVO.
Prevalence and Clinical Characteristics of Hearing Loss Caused by MYH14 Variants
Authors: Hiramatsu Ken , Nishio Shin-ya , Kitajiri Shin-ichiro , Kitano Tomohiro , Moteki Hideaki , Usami Shin-ichi ,   on behalf of the Deafness Gene Study Consortium
Published: October 2021

Variants in MYH14 are reported to cause autosomal dominant nonsyndromic hereditary hearing loss (ADNSHL), with 34 variants reported to cause hearing loss in various ethnic groups. However, the available information on prevalence, as well as with regard to clinical features, remains fragmentary. In this study, genetic screening for MYH14 variants was carried out using a large series of Japanese hearing-loss patients to reveal more detailed information. Massively parallel DNA sequencing of 68 target candidate genes was applied in 8074 unrelated Japanese hearing-loss patients (including 1336 with ADNSHL) to identify genomic variations responsible for hearing loss. We identified 11 families with 10 variants. The prevalence was found to be 0.14% (11/8074) among all hearing-loss patients and 0.82% (11/1336) among ADNSHL patients. Nine of the eleven variants identified were novel. The patients typically showed late-onset hearing loss arising later than 20 years of age (64.3%, 9/14) along with progressive (92.3%, 12/13), moderate (62.5%, 10/16), and flat-type hearing loss (68.8%, 11/16). We also confirmed progressive hearing loss in serial audiograms. The clinical information revealed by the present study will contribute to further diagnosis and management of MYH14-associated hearing loss.
Effects of Somatic, Depression Symptoms, and Sedentary Time on Sleep Quality in Middle-Aged Women with Risk Factors for Cardiovascular Disease
Authors: Choi Hyun-Sook , Kim Kyung-Ae
Published: October 2021

Cardiovascular disease (CVD) is the second leading cause of death among Korean women, and its incidence is dramatically elevated in middle-aged women. This study aimed to identify the predictors of sleep quality, a CVD risk factor, in middle-aged women with CVD risk factors to provide foundational data for developing intervention strategies for the prevention of CVD. The subjects, 203 middle-aged women (40–65 years old) with one or more CVD risk factors were selected through convenience sampling and included in this descriptive correlational study. The effects of somatic symptoms, depression symptoms, and sedentary time on sleep quality were examined. CVD-related characteristics were analyzed using descriptive statistics, whereas the mean values of the independent variables were analyzed using t-tests and analysis of variance. Predictors of sleep quality were analyzed using multiple regression analysis. The results showed that sleep quality increased with decreasing somatic symptoms (β = −0.36, p < 0.001), depression symptom score (β = −0.17, p = 0.023), and daily sedentary time (β = −0.13, p = 0.041), and the regression model was significant (F = 19.80, p < 0.001). Somatic symptoms are the most potent predictors of sleep quality in middle-aged women. Thus, intervention strategies that improve somatic symptoms are crucial for the enhancement of sleep quality, which deteriorates with advancing age.
User Perception of Automated Dose Dispensed Medicine in Home Care: A Scoping Review
Authors: Mertz Lasse , Tornbjerg Kristina , Nøhr Christian
Published: October 2021

(1) Background: Automated dose dispensing (ADD) systems are today used around the world. The ADD robots are placed in patients’ homes to increase medication safety as well as medication adherence; however, little is known about how ADD robots affect the patient’s day-to-day lives, receiving the daily doses of medicine from a machine rather than from a human healthcare professional. The aim of this study is to review the available literature on users’ perceptions of having an ADD robot and collect evidence on how they perceive having less human contact after implementing this technology in their homes. (2) Methods: References were searched for in Embase and PubMed. Literature investigating ADD robots in primary healthcare was included in this study and literature in a hospital setting was excluded. After screening processes, eleven publications were included in this review. (3) Results: The literature reported high medication adherence when using ADD robots and general satisfaction in terms of user experiences with the acceptability and functionality of ADD. (4) Conclusion: The review is the first focusing on user experience and perceptions regarding ADD robots. General satisfaction was shown towards ADD robots as an intervention, but the review indicates that research is missing on healthcare professionals and patient perceptions on how ADD affects their routines, both in relation to work and daily life.