bluuebunny/crossref_metadata_2025 huggingface dataset:
Growth Response of Trees with Different Growth Statuses to Pruning on a Pinus massoniana Lamb. Plantation
Authors: Zhao Xizhou , Mang Shunlan , Quan Wenxuan , Ding Guijie
Published: March 2023

Pruning is an important technique in culturing good knot-free timber. However, to make more accurate pruning plans, it is necessary to consider the growing status of trees and set reasonable pruning intensities based on this. In a seven-year-old Pinus massoniana Lamb. plantation, we carried out pruning twice with a time interval of two years. The treatments included one unpruned treatment (CK) and five pruned treatments (from P1 to P5, representing the live branch height being kept at 68%, 55%, 60%, 55% and 45% of the tree height). CK, P1 and P2 were conducted in the first pruning in March 2019, and the remaining treatments were conducted in the second pruning in January 2021. The growth investigations were carried out in March 2019, December 2019, December 2020 and December 2021. Another investigation was carried out only for the measurement of live branch heights and crown widths in January 2021 just after the second pruning. The results showed that pruning resulted in a 15.08% to 60.62% increase in diameter growth and an 10.28% to 29.87% increase in volume growth. The stem form was also improved. Significant differences were recorded in live branch heights after green pruning but gradually recovered to the same level in two years by branch senescence. Pruning also resulted in a faster extension of the crown width with an enlarged growing space by the removal of green branches. We highlighted that trees with different growth statuses responded differently to pruning intensities: trees of weak growth statuses grew better under a light pruning intensity, while those with a strong growth status grew better under a severe pruning intensity. Overall, for the seven-year-old young mason pine plantation, keeping four rounds of branches in trees with diameters of less than 8 cm and keeping three rounds of branches in trees with diameters of more than 8 cm were appropriate measures.
Mechanism of Terrestrial Plant Community Assembly under Different Intensities of Anthropogenic Disturbance in Dianchi Lakeside
Authors: Liu Zhen-Dian , Zhou Xiong-Li , Tian Jian-Juan , Yang Liu , Wang Yue-Hua , Shen Shi-Kang
Published: March 2023

A lakeside is a functional transition zone that connects the lake aquatic ecosystem and the land ecosystem. Understanding the community assembly mechanism is crucial for regional ecological restoration, habitat management, and biodiversity conservation. However, research on the terrestrial plant community assembly in lakesides under anthropogenic disturbance is still lacking. The present study used phylogeny and functional traits to assess the community assembly of three habitat types with different anthropogenic disturbances in Dianchi lakeside. The factors that influenced the community assembly were also explored. Results indicated that the phylogenetic signals of all the examined functional traits of the dominant species were weak, suggesting that the traits were convergent. The community phylogenetic and functional structures of the different habitat types showed random patterns. Thus, the assembly of terrestrial plant communities in the three habitat types was driven by competitive exclusion and neutral processes in Dianchi lakeside. The trait trade-off strategies of species in the different habitats varied with the different habitat types. Anthropogenic disturbance played an important role in the process of community assembly. The present study provides a scientific basis for the assessment and management of ecological restoration in Dianchi lakeside and other plateau lakes and enriches the knowledge on the community assembly mechanism of disturbed plant communities.
Structure and Ecosystem Services of Three Common Urban Tree Species in an Arid Climate City
Authors: Amer Alaa , Franceschi Eleonora , Hjazin Amgad , Shoqeir Jawad H. , Moser-Reischl Astrid , Rahman Mohammad A. , Tadros Maher , Pauleit Stephan , Pretzsch Hans , Rötzer Thomas
Published: March 2023

Urban forests play a critical role in improving the quality of life in cities, but in arid environments, little is known about the potential benefits and growth conditions of different tree species. Our study aimed to fill this gap by investigating the relationships between tree dimensions, above-ground biomass carbon storage, and shading potential in three common urban trees in the arid city of Jericho, Palestine, (i.e., Ficus nitida, Delonix regia, and Phoenix dactylifera). The trees were chosen according to their distribution in urban locations and tree vitality, with ages ranging from 20 to 90 years. Based on the results from tree structure measurements, the carbon storage and shading potential were calculated using the City Tree model. The results indicate a moderate to strong relationship between tree height, crown diameter, and crown volume for F. nitida and D. regia (R2 = 0.28–0.66), but no relationship for P. dactylifera (R2 = 0.03–0.06). The findings suggest that the analyzed tree species can considerably contribute to the potential benefits of trees in improving the climate of an arid city: D. regia shows a higher median of above-ground biomass carbon storage of 155 kg C tree−1, while P. dactylifera 91 kg C and F. nitida 76 Kg C. D. regia and F. nitida have a higher median of shading potential, (31 m2–41 m2), respectively. Information on the ecosystem services from urban trees and their relationships in terms of species, age, and tree planting urban location are very important for city planners, in relation to sustainable urban green spaces in arid cities.
Canopy Gaps Control Litter Decomposition and Nutrient Release in Subtropical Forests
Authors: Chen Jiajia , Zhu Jing , Wang Ziwei , Xing Cong , Chen Bo , Wang Xuelin , Wei Chensi , Liu Jinfu , He Zhongsheng , Xu Daowei
Published: March 2023

The formation of a canopy gap results in changes to the microenvironment which, in turn, affect litter decomposition and nutrient release. However, the mechanisms underlying these effects in differently sized gaps and non-gaps remain poorly understood. To address this gap in knowledge, we selected three large gaps (above 150 m2), three medium gaps (50–100 m2), three small gaps (30–50 m2), and three non-gaps with basically the same site conditions. We then used the litter bag method to investigate leaf and branch litter decomposition over a year in a Castanopsis kawakamii natural forest with the aim of characterizing the litter mass remaining and the nutrient release in canopy gaps and non-gaps. Our results revealed that the remaining litter mass of leaf and branch litter was lower in medium gaps compared to other gaps, and leaf litter decomposed faster than branch litter. Environmental factors were identified as the primary drivers of total carbon and nitrogen release during litter decomposition. Gap size (canopy openness), taxonomic Margalef index, the Brillouin index of soil microbes, soil total nitrogen content, soil pH value, and average air temperature were identified as the main factors driving carbon and nitrogen release from branch litter. In the late decomposition stage, the taxonomic Pielou index, soil total potassium content, soil water content, and average relative air humidity were the main drivers of nutrient release from branch litter. The soil water content and average relative air humidity were also found to be the main factors affecting the nutrient release from leaf litter throughout the different stages of decomposition. Overall, our study highlights the impact of canopy gaps on microenvironmental variation, taxonomic community diversity, and soil microbial functional diversity and how these factors ultimately influence litter decomposition and nutrient release. Our findings provide an important foundation for further research into soil nutrient cycling in subtropical natural forests.
Nutrient Resorption and Stoichiometric Characteristics of Wuyi Rock Tea Cultivars
Authors: Zhu Dehuang , Peng Suhong , Liu Wenzhen , Yu Shengjie , Hui Dafeng
Published: March 2023

Nutrient resorption is an important strategy for plants to retain critical nutrients from senesced leaves and plays important roles in nutrient cycling and ecosystem productivity. As a main economic crop and soil and water conservation species, Wuyi Rock tea has been widely planted in Fujian Province, China. However, foliar nutrient resorptions of Wuyi Rock tea cultivars have not been well quantified. In this study, three Wuyi Rock tea cultivars (Wuyi Jingui, Wuyi Rougui, and Wuyi Shuixian) were selected in the Wuyishan National Soil and Water Conservation, Science and Technology Demonstration Park. Resorption efficiencies of nitrogen (NRE), phosphorus (PRE), and potassium (KRE) along with their stoichiometric characteristics were determined. PRE of the three tea cultivars was significantly higher than KRE and NRE, indicating that tea cultivars were P limited due to low P availability for the tea growth. With the exception of Wuyi Rougui, leaf N and P contents of the other two cultivars (Wuyi Jingui and Wuyi Shuixian) had strong homeostasis under the changing soil environments. Leaf thickness and specific leaf area were positively and significantly correlated with KRE, and total chlorophyll concentration was positively correlated with NRE, indicating that leaf functional traits can be used as indicators for nutrient resorption status. Wuyi Rock tea cultivars had strong adaptabilities to the environments and had high carbon sequestration capabilities; thus, they and could be introduced into nutrient-poor mountainous areas for both economic benefits and soil and water conservation.
Constructing a Comprehensive National Wildfire Database from Incomplete Sources: Israel as a Case Study
Authors: Guk Edna , Bar-Massada Avi , Levin Noam
Published: March 2023

In many regions, the frequency and extent of wildfires has increased in recent years, a trend which is expected to continue. Hence, there is a need for effective fire management strategies. Such strategies need to be based on accurate and complete data on vegetation condition and post-fire effects, collected in the field as well as by remote sensing approaches. Unfortunately, wildfire databases are often incomplete in terms of their spatial and temporal coverage, as well as the documentation of fire outcomes. The aim of this study was to devise a methodology to construct a comprehensive national wildfire database. We developed and tested this methodology for Israel, as a case study. The methodology combines data on wildfires in Israel from two sources: remote sensing and field data collected by governmental agencies, representing the period between 2015 and 2022. The resulting database contains 2276 wildfires larger than 10 ha with information (where and when available) on their occurrence date, fire duration, ignition cause, and more. The methodology presented here provides a blueprint for developing large-scale and comprehensive fire databases elsewhere, and facilitates future studies on wildfire risk by providing a robust, unified database of the fire history of Israel from 2015 onwards.
Smoke Control in High-Rise Residential Buildings with Stair Pressurization Systems
Authors: Lee Ann , Lau Ghar Ek
Published: March 2023

Stair pressurization systems are designed to create a smoke-proof barrier, preventing the ingress of smoke into staircases within buildings. In tall buildings over 25 m, evacuation strategies can utilize phased or simultaneous evaluation of multiple storeys. This paper examines the performance of a stair pressurization with a differing number of doors open starting from two doors, with an incremental step of two up to a total of 10 doors into the shaft. Simulations using Fire Dynamics Simulation (FDS) have shown that a system that would pass commissioning requirements allows smoke in the shaft if there are more than four doors open simultaneously. The required airflow to prevent smoke ingress was found to be above an average flow speed of 0.7 m/s. Past this limit, the amount of smoke that leaks into the staircases continues to increase as more doors open. The study suggests that standards regarding stair pressurization systems should be updated to account for realistic evacuation scenarios, including the system’s cascade settings, number of storeys, and expected evacuation time, as evidenced by the smoke leakage once four doors were open and the drop-in flow rate between two-door and four-door open cases.
Phenotypic Stock Evaluation of Plagioscion magdalenae (Steindachner, 1878): A Species in the Dique Channel in Colombia
Authors: Hernandez Jordan , Correa Margarita , Hernández-P Raquel , Bermúdez Adriana , Quintana-Canabal Adriana , Laroze David , Benítez Hugo A.
Published: March 2023

Inland fishing is an essential activity for the livelihood and food security the Colombian population. The knowledge and evaluation of exploited fish stocks is a priority to develop sustainable management and conservation strategies of the fisheries. To optimize the management processes of fishery resources and conservation of species, it is necessary to evaluate the population structure and identification of stocks. Geometric morphometrics analysis have shown useful in the evaluation of fish stocks. This study focuses on the species Plagioscion magdalenae, commonly called “Pacora”, corvinata, or river croaker, which belongs to the family Sciaenidae, a family characterized as an important fishery resource. With the aim of generating a baseline about the state of the P. magdalenae population structure, samples were collected along the marshy complex of the Dique channel, Colombia, between December 2020 and October 2021. In this study, the existence of morphometric variability between individuals of Plagioscion magdalenae was found across sampling sites, Ciénaga de Capote and Ciénaga del Jobo; shape differences between location suggest the action of environmental pressures and the existence of anthropogenic pressures, such as unsustainable artisanal fishing.
Transition to Equilibrium and Coherent Structure in Ideal MHD Turbulence
Authors: Shebalin John V.
Published: March 2023

Transition of ideal, homogeneous, incompressible, magnetohydrodynamic (MHD) turbulence to near-equilibrium from non-equilibrium initial conditions is examined through new long-time numerical simulations on a 1283 periodic grid. Here, we neglect dissipation because we are primarily concerned with behavior at the largest scale which has been shown to be essentially the same for ideal and real (forced and dissipative) MHD turbulence. A Fourier spectral transform method is used to numerically integrate the dynamical equations forward in time and results from six computer runs are presented with various combinations of imposed rotation and mean magnetic field. There are five separate cases of ideal, homogeneous, incompressible, MHD turbulence: Case I, with no rotation or mean field; Case II, where only rotation is imposed; Case III, which has only a mean magnetic field; Case IV, where rotation vector and mean magnetic field direction are aligned; and Case V, which has nonaligned rotation vector and mean field directions. Dynamic coefficients are predicted by statistical mechanics to be zero-mean random variables, but largest-scale coherent magnetic structures emerge in all cases during transition; this implies dynamo action is inherent in ideal MHD turbulence. These coherent structures are expected to occur in Cases I, II and IV, but not in Cases III and V; future studies will determine whether they persist.
Bread Wheat Biofortification for Grain Carotenoid Content by Inter-Specific Breeding
Authors: Requena-Ramírez María Dolores , Rodríguez-Suárez Cristina , Ávila Carmen M. , Palomino Carmen , Hornero-Méndez Dámaso , Atienza Sergio G.
Published: March 2023

Bread wheat has traditionally been selected for whitish derived flours. As a consequence, the current varieties carry carotenogenic alleles associated with low grain carotenoid. In contrast, high grain yellow pigment content (YPC) has been a major target in durum wheat programs since yellow colour is an important aesthetic factor for pasta production. Phytoene synthase 1 (Psy1) genes have an important role in the determination of the carotenoid content in wheat. In this work, we have transferred the genes Psy1-A1 and Psy1-B1 from durum to bread wheat by inter-specific hybridization in order to evaluate the combined effect of these genes for the improvement of grain carotenoid content, as well as the development of carotenoid-enriched bread wheat lines. Inter-specific breeding coupled with a MAS approach based on Psy1-A1 and Psy1-B1 alleles has allowed the development of bread wheat pre-breeding lines with enhanced grain carotenoid content (16–23% mean). These biofortified lines have the potential to become new varieties or to be used as recurrent parents in bread wheat breeding programs.
Rapid and Sensitive Fluorescence Detection of Staphylococcus aureus Based on Polyethyleneimine-Enhanced Boronate Affinity Isolation
Authors: Xu Yujia , Zheng Hongwei , Sui Jianxin , Lin Hong , Cao Limin
Published: March 2023

There are increasing demands for fast and simple detection of pathogens in foodstuffs. Fluorescence analysis has demonstrated significant advantages for easy operation and high sensitivity, although it is usually hindered by a complex matrix, low bacterial abundance, and long-term bacterial enrichment. Effective enrichment procedures are required to meet the requirements for food detection. Here, boronate-functionalized cellulose filter paper and specific fluorescent probes were combined. An integrated approach for the enrichment of detection of Staphylococcus aureus was proposed. The modification of polyethyleneimine demonstrated a significant effect in enhancing the bacterial enrichment, and the boronate affinity efficiency of the paper was increased by about 51~132%. With optimized conditions, the adsorption efficiency for S. aureus was evaluated as 1.87 × 108 CFU/cm2, the linear range of the fluorescent analysis was 104 CFU/mL~108 CFU/mL (R2 = 0.9835), and the lowest limit of detection (LOD) was calculated as 2.24 × 102 CFU/mL. Such efficiency was validated with milk and yogurt samples. These results indicated that the material had a high enrichment capacity, simple operation, and high substrate tolerance, which had the promising potential to be the established method for the fast detection of food pathogens.
Quantification of Free Short-Chain Fatty Acids in Raw Cow Milk by Gas Chromatography-Mass Spectrometry
Authors: Wu Xufang , Wang Fengen , Chen Meiqing , Wang Jiaqi , Zhang Yangdong
Published: March 2023

Free short-chain fatty acids (FSCFAs) are a momentous contributor to the flavor of the raw cow milk. Hence, the purpose of this research was to build an approach for the quantification of 10 FSCFAs in raw cow milk. Raw cow milk samples are acidified by hydrochloric acid ethanol (0.5%) solution pretreatment and then processed on the gas chromatography-mass spectrometry. With the exception of iso C5:0 and anteiso C5:0 co-flux, the remaining eight FSCFAs were effectively separated by chromatography. The methodological validation data revealed that the linear relationship satisfied the assay requirements (coefficient of determination >0.999), the limits of quantification were 0.167 to 1.250 μg mL−1, the recoveries ranged from 85.62% to 126.42%, the coefficients of variation were 1.40~12.15%, and no SCFAs in the triglyceride form were potential degradation, and the precision ranging from 0.56% to 9.09%. Our easy, fast, and robust method successfully determined three FSCFAs in raw cow milk without derivatization. Some characteristic features of FSCFAs have been discovered in raw cow milk such as its higher percentages of C4:0 and C6:0. Our research has provided a very valuable method for the future quality and safety control of raw milk and nutritional studies.
Anthocyanins: Modified New Technologies and Challenges
Authors: Lin Yang , Li Cong , Shi Lejuan , Wang Lixia
Published: March 2023

Anthocyanins are bioactive compounds belonging to the flavonoid class which are commonly applied in foods due to their attractive color and health-promoting benefits. However, the instability of anthocyanins leads to their easy degradation, reduction in bioactivity, and color fading in food processing, which limits their application and causes economic losses. Therefore, the objective of this review is to provide a systematic evaluation of the published research on modified methods of anthocyanin use. Modification technology of anthocyanins mainly includes chemical modification (chemical acylation, enzymatic acylation, and formation of pyran anthocyanidin), co-pigmentation, and physical modification (microencapsulation and preparation of pickering emulsion). Modification technology of anthocyanins can not only increase bioavailability and stability of anthocyanin but also can improve effects of anthocyanin on disease prevention and treatment. We also propose potential challenges and perspectives for diversification of anthocyanin-rich products for food application. Overall, integrated strategies are warranted for improving anthocyanin stabilization and promoting their further application in the food industry, medicine, and other fields.
Burn Defect and Phenol Prediction for Flavoured Californian-Style Black Olives Using Digital Sensors
Authors: Cascos Gema , Barea-Ramos Juan , Montero-Fernández Ismael , Ruiz-Canales Antonio , Lozano Jesús , Martín-Vertedor Daniel
Published: March 2023

Californian-style black olives can undergo different chemical changes during the sterilization process that can affect their sensory and phenol characteristics. Thus, these olives were stuffed with flavoured hydrocolloids and submitted to different thermal sterilization treatments to assess sensory categories. The triangular test indicated that the panellists were able to discriminate between samples from different categories according to their aromas with more than 85% success. The results indicated that the negative aroma detected by tasters was related to burn defects. The highest level of defects was found in standard olives, while the lowest was identified in the extra category. Furthermore, olives submitted to the lowest thermal sterilization treatment (extra) presented significantly higher phenol profile content, such as for hydroxytyrosol, tyrosol, oleuropein and procyanidin B1. The electronic nose (E-nose) discriminated between samples from different categories according to the specific aroma (PC1 = 82.1% and PC2 = 15.1%). The PLS-DA classified the samples with 90.9% accuracy. Furthermore, the volatile organic compounds responsible for this discrimination were creosol, copaene, benzaldehyde and diallyl disulphide. Finally, the models established by the PLS analysis indicated that the E-nose could predict olives according to their aroma and total phenol profile (RCV2 values were 0.89 and 0.92, respectively). Thus, this device could be used at the industrial level to discriminate between olives with different sensory aromas to determine those with the highest quality.
Bamboo Shoots Modulate Gut Microbiota, Eliminate Obesity in High-Fat-Diet-Fed Mice and Improve Lipid Metabolism
Authors: Zhou Xiaolu , Pak SolJu , Li Daotong , Dong Li , Chen Fang , Hu Xiaosong , Ma Lingjun
Published: March 2023

Bamboo shoots (BS) have a variety of nutritional benefits; however, their anti-obesity effect and its underlying mechanism of action are still unclear. In this study, we investigated the protective effect of BS against high-fat diet (HFD)-induced gut dysbiosis in mice. After 12 weeks of feeding C57BL/6J mice either on a normal or an HFD with or without BS, metabolic indicators, including blood lipids and glucose tolerance, were measured. 16S rRNA gene sequencing and metabolomics were used to identify alterations in gut microbiota composition and fecal metabolic profiling. The results demonstrated that BS supplementation reduced body weight by 30.56%, mitigated liver damage, and improved insulin resistance and inflammation in obese mice. In addition, BS increased short-chain fatty acid (SCFA) levels and SCFA-producing bacteria (e.g., Lachnospiraceae_NK4A136_group and Norank_f_Muribaculaceae), and reduced levels of harmful bacteria (e.g., Blautia and Burkholderia–Paraburkholderia). Finally, BS increased many beneficial fecal metabolites, such as fatty acids and bile acids, which are highly relevant to the altered gut microbiota. Based on the modulatory effect of BS on microbiota composition and gut metabolite levels observed in this study, we suggest that BS may be beneficial in treating obesity and its related complications.
Effect of Serum and Oxygen on the In Vitro Culture of Hanwoo Korean Native Cattle-Derived Skeletal Myogenic Cells Used in Cellular Agriculture
Authors: Ock Sun A , Seo Kang-Min , Ju Won Seok , Kim Young-Im , Wi Ha-Yeon , Lee Poongyeon
Published: March 2023

Skeletal muscle-derived myogenic cells (SKMCs) are novel protein sources capable of replacing animal meat. However, SKMCs have not been commercialized owing to poor productivity and the high cost of in vitro cell culture. Therefore, we cultured SKMCs in varying serum (5–20%) and oxygen concentrations (5–20%) to investigate the parameters that most impact cell productivity (serum, hypoxia, and culture medium) and examined cell proliferation ability and genes involved in myogenesis/proliferation/apoptosis/reactive oxygen species (ROS). In fetal bovine serum (FBS) groups, hypoxia induction doubled cell number, and the 20% FBS/normoxia group exhibited similar cell numbers as 5% FBS/5% hypoxia, confirming that 5% hypoxia reduced serum requirement by four-fold. The use of 20% FBS downregulated MTF5/MYOD1/MYOG/MYH1, whereas hypoxia induction with ≤10% FBS upregulated them. Although 20% FBS lowered TERT expression through rapid cell proliferation, NOX1, a major factor of ROS, was suppressed. DMEM/F12 demonstrated better differentiation potential than F10 by upregulating MYF3/MYOD1/MYOG/MYH1 and downregulating MSTN, particularly DMEM/F12 with 2% FBS/5% hypoxia. The myogenic fusion index was higher in DMEM/F12 without FBS than in DMEM/F12 with FBS (0.5–5%); however, the total nuclei number was reduced owing to apoptosis. Therefore, high serum levels are essential in influencing SKMC growth, followed by hypoxia as a synergistic component.
Close Ties between the Nuclear Envelope and Mammalian Telomeres: Give Me Shelter
Authors: Pennarun Gaëlle , Picotto Julien , Bertrand Pascale
Published: March 2023

The nuclear envelope (NE) in eukaryotic cells is essential to provide a protective compartment for the genome. Beside its role in connecting the nucleus with the cytoplasm, the NE has numerous important functions including chromatin organization, DNA replication and repair. NE alterations have been linked to different human diseases, such as laminopathies, and are a hallmark of cancer cells. Telomeres, the ends of eukaryotic chromosomes, are crucial for preserving genome stability. Their maintenance involves specific telomeric proteins, repair proteins and several additional factors, including NE proteins. Links between telomere maintenance and the NE have been well established in yeast, in which telomere tethering to the NE is critical for their preservation and beyond. For a long time, in mammalian cells, except during meiosis, telomeres were thought to be randomly localized throughout the nucleus, but recent advances have uncovered close ties between mammalian telomeres and the NE that play important roles for maintaining genome integrity. In this review, we will summarize these connections, with a special focus on telomere dynamics and the nuclear lamina, one of the main NE components, and discuss the evolutionary conservation of these mechanisms.
Correlation between Parental Transcriptome and Field Data for the Characterization of Heterosis in Chinese Cabbage
Authors: Li Ru , Tian Min , He Qiong , Zhang Lugang
Published: March 2023

In Chinese cabbage breeding, hybrids have made a terrific contribution due to heterosis, the superior performance of offspring compared to their inbred parents. Since the development of new, top-performing hybrids requires a large scale of human and material resources, the prediction of hybrid performance is of utmost interest to plant breeders. In our research, leaf transcriptome data from eight parents were used to investigate if they might be employed as markers to predict hybrid performance and heterosis. In Chinese cabbage, heterosis of plant growth weight (PGW) and heterosis of head weight (HW) were more obvious than other traits. The number of differential expression genes (DEGs) between parents was related to the PGW, length of the biggest outer leaf (LOL), leaf head height (LHH), leaf head width (LHW), HW, leaf number of head (LNH) and plant height (PH) of hybrids, and up-regulated DEGs number was also associated with these traits. Euclidean and binary distances of parental gene expression levels were significantly correlated with the PGW, LOL, LHH, LHW, HW and PH of hybrids. Additionally, there was a significant correlation between the parental expression levels of multiple genes involved in the ribosomal metabolic pathway and hybrid observations and heterosis in PGW, with the BrRPL23A gene showing the highest correlation with the MPH of PGW(r = 0.75). Therefore, leaf transcriptome data can preliminarily predict the hybrid performance and select parents in Chinese cabbage.
Unboxing Industry-Standard AI Models for Male Fertility Prediction with SHAP
Authors: GhoshRoy Debasmita , Alvi Parvez , Santosh KC
Published: March 2023

Infertility is a social stigma for individuals, and male factors cause approximately 30% of infertility. Despite this, male infertility is underrecognized and underrepresented as a disease. According to the World Health Organization (WHO), changes in lifestyle and environmental factors are the prime reasons for the declining rate of male fertility. Artificial intelligence (AI)/machine learning (ML) models have become an effective solution for early fertility detection. Seven industry-standard ML models are used: support vector machine, random forest (RF), decision tree, logistic regression, naïve bayes, adaboost, and multi-layer perception to detect male fertility. Shapley additive explanations (SHAP) are vital tools that examine the feature’s impact on each model’s decision making. On these, we perform a comprehensive comparative study to identify good and poor classification models. While dealing with the all-above-mentioned models, the RF model achieves an optimal accuracy and area under curve (AUC) of 90.47% and 99.98%, respectively, by considering five-fold cross-validation (CV) with the balanced dataset. Furthermore, we provide the SHAP explanations of existing models that attain good and poor performance. The findings of this study show that decision making (based on ML models) with SHAP provides thorough explanations for detecting male fertility, as well as a reference for clinicians for further treatment planning.
Dynamic Trends in Sociodemographic Disparities and COVID-19 Morbidity and Mortality—A Nationwide Study during Two Years of a Pandemic
Authors: Kaim Arielle , Saban Mor
Published: March 2023

Social epidemiological research has documented that health outcomes, such as the risk of becoming diseased or dying, are closely tied to socioeconomic status. The aim of the current study was to investigate the impact of socioeconomic status on morbidity, hospitalization, and mortality outcomes throughout five waves of the pandemic amongst the Israeli population. A retrospective archive study was conducted in Israel from March 2020 to February 2022 in which data were obtained from the Israeli Ministry of Health’s (MOH) open COVID-19 database. Our findings, though requiring careful and cautious interpretation, indicate that the socioeconomic gradient patterns established in previous COVID-19 literature are not applicable to Israel throughout the five waves of the pandemic. The conclusions of this study indicate a much more dynamic and complex picture, where there is no single group that dominates the realm of improved outcomes or bears the burden of disease with respect to morbidity, hospitalization, and mortality. We show that health trends cannot necessarily be generalized to all countries and are very much dynamic and contingent on the socio-geographical context and must be thoroughly examined throughout distinct communities with consideration of the specific characteristics of the disease. Furthermore, the implications of this study include the importance of identifying the dynamic interplay and interactions of sociodemographic characteristics and health behavior in order to enhance efforts toward achieving improved health outcomes by policymakers and researchers.
Manual and Application-Based Carbohydrate Counting and Glycemic Control in Type 1 Diabetes Subjects: A Narrative Review
Authors: AlBabtain Sara A. , AlAfif Nora O. , AlDisi Dara , AlZahrani Saad H.
Published: March 2023

Type 1 diabetes (T1DM) is the most common chronic disease in young adults and children, which is treated with insulin, usually given as basal and boluses. Carbohydrate counting (CHOC) helps patients to determine the correct meal doses. The aim of this review is to study the effect of CHOC on glucose control, body weight, insulin dose and quality of life (QoL). The literature search was conducted using PubMed from January 2010 to October 2022. Studies included in this review are limited to randomized controlled studies involving an intervention group undergoing CHOC and a control group following the usual practice, measuring glycosylated hemoglobin (HbA1c) as a parameter of glucose control and involving only T1DM subjects. A total of ten articles were found to fulfill the criteria involving 1034 patients. Most of the studies showed a positive impact of CHOC on glucose control, especially in adults, where five out of six studies were statistically positive. However, in pediatrics, only two out of four showed a positive outcome. In all four studies using mobile applications, CHOC was better at controlling glucose. No difference was seen between the CHOC group and the control regarding the risk of severe hypoglycemia. In fact, two studies have shown lower hypoglycemia rates. No change in weight was observed in most of the studies (six out of eight). In subjects with T1DM, CHOC might provide better glucose control than traditional care without a significant increment in severe hypoglycemia or weight gain. Mobile application-based models showed promising results in glucose control.
Perceptions of and Satisfaction with Sexual and Reproductive Health Interventions in Pre-Adolescent and Adolescent Students in UE/EEA Countries: A Systematic Review
Authors: Montalti Marco , Longobucco Yari , Celani Chiara , Dallolio Laura , Masini Alice
Published: March 2023

The objective of this review is to investigate perceptions of and satisfaction with sexual and reproductive health (SRH) interventions among pre-adolescents and adolescents of all genders and ethnicities in EU/EEA countries. This systematic review was conducted in accordance with PRISMA recommendations. A systematic literature search was conducted on MEDLINE (PubMed), Cochrane Central Register of Controlled Trials (Central), CINAHL (EBSCO), and PsycINFO (EBSCO) up to March 2022 to identify all published articles that included information on perceptions and levels of satisfaction with SRH interventions. The selected studies were independently and blindly evaluated for risk of bias. Finally, only five papers were included in our review, divided according to the implemented intervention design: conducted by research groups (health or non-health professionals) or structured entirely in a peer-to-peer approach. Both types of program designs analyzed in the included studies were well accepted by students, even though satisfaction assessment methods were not standardized. Peer-to-peer conducted SRH programs or interventions with practical components (e.g., exercises, discussion) were more appreciated. We also found higher levels of satisfaction among younger participants. Future SRH educational programs should consider an assessment of participants’ perceptions and satisfaction, possibly adopting standardized tools. Following a peer-to-peer structure and delivering programs early could lead to greater participant satisfaction.
Challenges, Concerns, and Experiences of Community-Dwelling Older Women with Chronic Low Back Pain—A Qualitative Study in Hong Kong, China
Authors: Wong Tiffany H. T. , Lee Kaden S. K. , Lo Sharon M. C. , Kan Mandy M. P. , Kwan Crystal , Opsommer Emmanuelle , Anwer Shahnawaz , Li Heng , Wong Arnold Y. L. , Schoeb Veronika
Published: March 2023

Background and Objectives: Although chronic low back pain (CLBP) is known to negatively affect multiple aspects of the lives of older people, prior qualitative studies mainly focused on the lived experiences of older people with CLBP in Western countries. Given cultural and contextual differences and poor understanding of CLBP in older women with CLBP, it is important to better understand the concerns and lived experiences of Chinese older women with CLBP. The current study aimed to investigate the experiences, challenges, concerns, and coping strategies of older women with CLBP in Hong Kong. Research Design and Methods: A total of 15 community-dwelling older women with CLBP aged ≥60 years were recruited from a physiotherapy clinic or a community center for semi-structured interviews. The interviews were audio recorded and transcribed ‘verbatim’. The transcription was imported to NVivo 12 software. Thematic analysis was conducted using Braun and Clarke’s method. Results: Five themes were identified: (1) physical impacts of CLBP on daily life; (2) psychological influences of CLBP; (3) management of CLBP; (4) family support; and (5) social activities and support. Discussion and implications: Negative physical and psychosocial impacts of CLBP were common among older women, and they adopted diverse pain management strategies, although some of their treatment options were influenced by the Chinese culture. Misbeliefs and responses of family and friends also affected their management strategies. Elderly community centers are a significant source of social support for older women with CLBP, making it an ideal platform for establishing self-help groups to facilitate their self-management of CLBP.
Massive Irreparable Rotator Cuff Tears: Which Patients Will Benefit from Physiotherapy Exercise Programs? A Narrative Review
Authors: Ó Conaire Eoin , Delaney Ruth , Lädermann Alexandre , Schwank Ariane , Struyf Filip
Published: March 2023

Massive irreparable rotator cuff tears can cause significant shoulder pain, disability and reduction in quality of life. Treatment approaches can be operative or non-operative. Operative approaches include reverse total shoulder arthroplasty, arthroscopic debridement, partial rotator cuff repair, subacromial balloon spacers, superior capsule reconstruction, and tendon transfer procedures. Non-operative approaches include physiotherapy exercise programs and corticosteroid injections. There are no randomized controlled trials comparing the different treatment approaches. It is therefore challenging for clinicians to advise patients on what is their best treatment pathway. Physiotherapy exercise programs are less expensive and have lower risks for patients than surgical approaches. However, the success of physiotherapy in patients with massive irreparable rotator cuff tears is highly variable with published success rates of 32–96%. Several cohort studies have sought to identify if certain factors are predictive of success with physiotherapy. Several biomechanical factors were identified as possibly being related to a successful or unsuccessful outcome following physiotherapy, with complete tear of subscapularis demonstrating the strongest evidence. However, there were no appropriately designed prognostic studies. There has been a strong emphasis on biomechanical factors. Other domains such as psychosocial factors, which are important in similar patient populations, have not been explored. We recommend that further research is needed in this area and should include randomized controlled trials comparing treatment approaches and longitudinal prospective prognostic studies to identify predictors of treatment success.
Smartphone Apps for Domestic Violence Prevention: A Systematic Review
Authors: Sumra Mehreen , Asghar Sohail , Khan Khalid S. , Fernández-Luna Juan M. , Huete Juan F. , Bueno-Cavanillas Aurora
Published: March 2023

Smartphone applications or apps are increasingly being produced to help with protection against the risk of domestic violence. There is a need to formally evaluate their features. Objective: This study systematically reviewed app-based interventions for domestic violence prevention, which will be helpful for app developers. Methods: We overviewed all apps concerning domestic violence awareness and prevention without language restrictions, collating information about features and limitations. We conducted searches in Google, the Google Play Store, and the App Store (iOS) covering a 10-year time period (2012–2022). We collected data related to the apps from the developers’ descriptions, peer reviewed research articles, critical reviews in blogs, news articles, and other online sources. Results: The search identified 621 potentially relevant apps of which 136 were selected for review. There were five app categories: emergency assistance (n = 61, 44.9%), avoidance (n = 29, 21.3%), informative (n = 29, 21.3%), legal information (n = 10, 7.4%), and self-assessment (n = 7, 5.1%). Over half the apps (n = 97, 71%) were released in 2020–22. Around a half were from north-east America (n = 63, 46.3%). Where emergency alerts existed, they required triggering by the potential victim. There was no automation. Content analysis showed 20 apps with unique features, including geo-fences, accelerometer-based alert, shake-based alert, functionality under low resources, alert auto-cancellation, anonymous communication, and data encryption. None of the apps deployed artificial intelligence to assist the potential victims. Conclusions: Apps currently have many limitations. Future apps should focus on automation, making better use of artificial intelligence deploying multimedia (voice, video, image capture, text and sentiment analysis), speech recognition, and pitch detection to aid in live analysis of the situation and for accurately generating emergency alerts.
Using Relational Community Engagement within the Digital Health Intervention (DHI) to Improve Access and Retention among People Living with HIV (PLWH): Findings from a Mixed-Method Study in Cambodia
Authors: Seang Kennarey , Ky Sovathana , Ngauv Bora , Mam Sovatha , Ouk Vichea , Saphonn Vonthanak
Published: March 2023

We examined the impact of COVID-19-associated restrictive measures on the HIV care system in Cambodia through a complexity lens and aimed to use the findings to integrate social and relational processes into the design and implementation of proposed solutions that could support program outcomes during these times. Through a mixed-method design, we generated data on the strength of connection and quality of relationships between stakeholders and how this, in turn, provided a more holistic understanding of the challenges experienced during a pandemic. We interviewed 43 HIV care providers and 13 patients from eight HIV clinics and 13 policy-level stakeholders from relevant institutions involved in HIV care from April to May 2021. We identified several challenges, as well as an opportunity to improve HIV care access that built upon a strong foundation of trust between the HIV care providers and receivers in Cambodia. Trusting relationships between providers and patients provided the basis for intervention development aiming to improve the care experience and patients’ engagement in care. Iterative research processes could better inform the intervention, and communication resources provided through relational skills training are key to their application and sustainability.
Characteristics of Fatal, Pedestrian-Involved, Motor Vehicle Crashes in West Virginia: A Cross-Sectional and Spatial Analysis
Authors: Rudisill Toni M. , Barbee Lauren Olivia , Hendricks Brian
Published: March 2023

Fatal, pedestrian-involved, motor vehicle collisions are increasing in the United States yet remain lower in rural states such as West Virginia. This study’s purpose was to investigate the overall risk factors of pedestrian fatalities by rurality and sex in West Virginia. Data were obtained from the Fatality Analysis Reporting System. The fatality had to occur within West Virginia between 1 January 2009 and 31 December 2019. Risk factors of rural vs. urban and male vs. female crashes were determined using multivariable logistic regression models. Clustering of crash locations was analyzed using kernel density estimation and Ripley’s K. Among the 254 fatalities, most victims were male (70%). Most crashes occurred at night (76%), on highways (73%), on level (71%), non-curved (84%), dry (82%) roads during fair weather conditions (82%). Nearly 34% of the victims tested positive for alcohol. Men were 2.5 times as likely to be hit in a rural area (OR = 2.5; 95% CI 1.2, 5.4), on curved roads, and 57% less likely (OR = 0.43; 95% CI 0.2, 0.9) to test positive for drugs compared to women. Crash characteristics, including location, were similar between the sexes. As many risk factors were modifiable behaviors, public health interventions to ensure pedestrian safety may be necessary.
National and Regional Rates of Chronic Diseases and All-Cause Mortality in Saudi Arabia—Analysis of the 2018 Household Health Survey Data
Authors: Alzahrani Majed S. , Alharthi Yaser S. , Aljamal Jamal K. , Alarfaj Abdulrahman A. , Vennu Vishal , Noweir Mohammed D.
Published: March 2023

The disease burden and mortality were estimated in Saudi Arabia between 2010 and 2017 but were unknown in 2018. This study aims to assess the 2018 national and regional rates of chronic diseases and all-cause mortality among the total and Saudi populations. In this descriptive cross-sectional study, we obtained data from 24,012 households from the 2018 household health survey. We included doctor-diagnosed chronic conditions such as diabetes mellitus (DM), hypertension (HTN), cardiovascular diseases (CAD), and cancer (CN). A secondary analysis was performed by the total and Saudi populations. Both citizens and residents comprised the total population. Makkah and Al-Medina had greater rates among the total population; however, Al-Baha and Ha’il had high rates of chronic diseases and related mortality in the Saudi population. Age-adjusted mortality rates were 286 per 100,000 population-year. The age-adjusted mortality rate among those aged 65 and above was 3428 per 100,000 population in the same age group. Men had a rate of 1779 per 100,000 men, which was higher than the rate of 1649 for women. In 2018, most citizens in Ha’il had DM, most Al-Baha had HTN and CAD, and most Al-Qassim had CN. People aged 65 and older had the highest death rate.