bluuebunny/crossref_metadata_2025 huggingface dataset:
Understanding the challenges and novel architectural models of multi-cloud native applications – a systematic literature review
Authors: Alonso Juncal , Orue-Echevarria Leire , Casola Valentina , Torre Ana Isabel , Huarte Maider , Osaba Eneko , Lobo Jesus L.
Published: January 2023

The evolution of Cloud Computing into a service utility, along with the pervasive adoption of the IoT paradigm, has promoted a significant growth in the need of computational and storage services. The traditional use of cloud services, focused on the consumption of one provider, is not valid anymore due to different shortcomings being the risk of vendor lock-in a critical. We are assisting to a change of paradigm, from the usage of a single cloud provider to the combination of multiple cloud service types, affecting the way in which applications are designed, developed, deployed and operated over such heterogeneous ecosystems. The result is an effective heterogeneity of architectures, methods, tools, and frameworks, copying with the multi-cloud application concept. The goal of this study is manifold. Firstly, it aims to characterize the multi-cloud concept from the application development perspective by reviewing existing definitions of multi-cloud native applications in the literature. Secondly, we set up the basis for the architectural characterization of these kind of applications. Finally, we highlight several open research issues drawn up from the analysis carried out. To achieve that, we have conducted a systematic literature review (SLR), where, a large set of primary studies published between 2011 and 2021 have been studied and classified. The in-depth analysis has revealed five main research trends for the improvement of the development and operation DevOps lifecycle of “multi-cloud native applications”. The paper finishes with directions for future work and research challenges to be addressed by the software community.
The development of social science research on smart grids: a semi-structured literature review
Authors: Kojonsaari A.-R. , Palm J.
Published: January 2023

Background

Smart technologies, such as smart grids, are emerging as indispensable aspects of an energy transformation and come with hopes of more sustainable resource use. A substantial amount of research has examined the technical, economic, and environmental implications of these technologies, but less attention has been paid to their social aspects. For the smart grid projects to be realised, studies that include the actors who are supposed to implement the visions are needed.

Results

A semi-structured literature review was conducted to investigate the state of social science literature on smart grids and identify the main research avenues and research gaps by addressing a broad research question: “What kind of knowledge is produced in social science studies on smart grids?” We retrieved peer-reviewed articles from the Scopus and Web of Science (WoS) databases up until 2022 and mapped them in terms of features such as topic, design, method, and theory.

Conclusions

We found that knowledge development in social science studies on smart grids followed a pattern where most research focused on visions; professionals and users; and smart technologies in homes with a geographical focus on Europe or the USA. We identified six research gaps related to an overly vague definition of the smart grid and the need to include more diverse actors and geographical places to advance our understanding of the smart grid. There is also a lack of studies relating to energy democracy, the resistance of smart grids and the centralised–decentralised nexus of the smart grid. These less studied areas can bring in new knowledge that enhances a deployment of a smart grids supporting not only technological development, but also society and users.
Searching for a prognostic index in lupus nephritis
Authors: Rodríguez-Almaraz E. , Gutiérrez-Solís E. , Rabadán E. , Rodríguez P. , Alonso M. , Carmona L. , de Yébenes M. J. García , Morales E. , Galindo-Izquierdo M.
Published: January 2023

Background

Currently we do not have an ideal biomarker in lupus nephritis (LN) that should help us to identify those patients with SLE at risk of developing LN or to determine those patients at risk of renal progression. We aimed to evaluate the development of a prognostic index for LN, through the evaluation of clinical, analytical and histological factors used in a cohort of lupus. We have proposed to determine which factors, 6 months after the diagnosis of LN, could help us to define which patients will have a worse evolution of the disease and may be, more aggressive treatment and closer follow-up.

Methods

A retrospective study to identify prognostic factors was carried out. We have included patients over 18 years of age with a clinical diagnosis of systemic lupus erythematosus (SLE) and kidney involvement confirmed by biopsy, who are followed up in our centre during the last 20 years. A multi-step statistical approach will be used in order to obtain a limited set of parameters, optimally selected and weighted, that show a satisfactory ability to discriminate between patients with different levels of prognosis.

Results

We analysed 92 patients with LN, although only 73 have been able to be classified according to whether or not they have presented poor renal evolution. The age of onset (44 vs. 32; p = 0.024), the value of serum creatinine (1.41 vs. 1.04; p = 0.041), greater frequency of thrombocytopenia (30 vs. 7%; p = 0.038), higher score in the renal chronicity index (2.47 vs. 1.04; p = 0.015), proliferative histological type (100%) and higher frequency of interstitial fibrosis (67 vs. 32%; p = 0.017) and tubular atrophy (67 vs. 32%; p = 0.018) was observed between two groups. The multivariate analysis allowed us to select the best predictive model for poor outcome at 6 months based on different adjustment and discrimination parameters.

Conclusion

We have developed a prognostic index of poor renal evolution in patients with LN that combines demographic, clinical, analytical and histopathological factors, easy to use in routine clinical practice and that could be an effective tool in the early detection and management.
The effects of dietary supplementation with mushroom or selenium enriched mushroom powders on the growth performance and intestinal health of post-weaned pigs
Authors: Dowley Alison , Sweeney Torres , Conway Eadaoin , Vigors Stafford , Ryan Marion T. , Yadav Supriya , Wilson Jude , O’Doherty John V.
Published: January 2023

Background

There is an urgent need to identify natural bioactive compounds that can enhance gastrointestinal health and promote pig growth performance in the absence of pharmacological levels of zinc oxide (ZnO). The objectives of this study were to: 1) compare the effects of mushroom powder supplemented with inorganic selenium (inSeMP) to mushroom powder enriched with organic selenium (orgSeMP) to pharmacological levels of ZnO on growth performance and faecal scores (FS) for the first 21 d post-weaning (Period 1); and 2) compare the molecular and microbial effects of inSeMP and orgSeMP in these pigs on d 39 post-weaning (Period 2).

Methods

In Period 1, pigs (3 pigs/pen; 8 pens/treatment) were assigned to: (1) basal diet (control); (2) basal diet + zinc oxide (ZnO) (3100 mg/kg d 1–14, 1550 mg/kg d 15–21); (3) basal diet + mushroom powder supplemented with inorganic selenium (inSeMP) containing selenium (selenite) content of 0.3 mg/kg feed; (4) basal diet + mushroom powder enriched with organic selenium (orgSeMP) containing selenium (selenocysteine) content of 0.3 mg/kg feed. Mushroom powders were included at 6.5 g/kg of feed.

Results

In Period 1, there was no effect of diets on average daily gain (ADG) and gain:feed (G:F) ratio (P > 0.05). The orgSeMP supplemented pigs had a lower average daily feed intake (ADFI) compared to all other groups (P < 0.05). The ZnO supplemented pigs had reduced FS compared to the basal and mushroom group, while the orgSeMP supplemented pigs had lower FS compared to the basal group during the 21 d experimental period (P < 0.05). In Period 2, there was no effect of diets on ADFI, ADG and G:F ratio (P > 0.05). The orgSeMP supplementation increased the caecal abundance of bacterial members of the Firmicutes and Bacteroidetes phylum, includingLactobacillus, Agathobacter, Roseburia,andPrevotellaand decreased the abundance ofSporobactercompared to the basal group, while inSeMP increased the caecal abundance ofPrevotellaand decreased the caecal abundance ofSporobactercompared to the basal group (P < 0.05). Dietary supplementation with inSeMP increased expression ofTLR4and anti-inflammatory cytokine geneIL10and decreased nutrient transporter geneFABP2compared to the orgSeMP group (P < 0.05).

Conclusion

OrgSeMP is a novel and sustainable way to incorporate selenium and β-glucans into the diet of weaned pigs whilst improving FS and modulating the caecal microbiota.
Nationwide genomic surveillance reveals the prevalence and evolution of honeybee viruses in China
Authors: Li Nannan , Li Cixiu , Hu Tao , Li Juan , Zhou Hong , Ji Jingkai , Wu Jiangli , Kang Weipeng , Holmes Edward C. , Shi Weifeng , Xu Shufa
Published: January 2023

Background

The economic and environmental value of honeybees has been severely challenged in recent years by the collapse of their colonies worldwide, often caused by outbreaks of infectious diseases. However, our understanding of the diversity, prevalence, and transmission of honeybee viruses is largely obscure due to a lack of large-scale and longitudinal genomic surveillance on a global scale.

Results

We report the meta-transcriptomic sequencing of nearly 2000 samples of the two most important economic and widely maintained honeybee species, as well as an associated ectoparasite mite, collected across China during 2016–2019. We document the natural diversity and evolution of honeybee viruses in China, providing evidence that multiple viruses commonly co-circulate within individual bee colonies. We also expanded the genomic data for 12 important honeybee viruses and revealed novel genetic variants and lineages associated with China. We identified more than 23 novel viruses from the honeybee and mite viromes, with some exhibiting ongoing replication in their respective hosts. Together, these data provide additional support to the idea that mites are an important reservoir and spill-over host for honeybee viruses.

Conclusions

Our data show that honeybee viruses are more widespread, prevalent, and genetically diverse than previously realized. The information provided is important in mitigating viral infectious diseases in honeybees, in turn helping to maintain sustainable productive agriculture on a global scale.
Identifying new biomarkers of aggressive Group 3 and SHH medulloblastoma using 3D hydrogel models, single cell RNA sequencing and 3D OrbiSIMS imaging
Authors: Linke Franziska , Johnson James E. C. , Kern Stefanie , Bennett Christopher D. , Lourdusamy Anbarasu , Lea Daniel , Clifford Steven C. , Merry Catherine L. R. , Stolnik Snow , Alexander Morgan R. , Peet Andrew C. , Scurr David J. , Griffiths Rian L. , Grabowska Anna M. , Kerr Ian D. , Coyle Beth
Published: January 2023

The most common malignant brain tumour in children, medulloblastoma (MB), is subdivided into four clinically relevant molecular subgroups, although targeted therapy options informed by understanding of different cellular features are lacking. Here, by comparing the most aggressive subgroup (Group 3) with the intermediate (SHH) subgroup, we identify crucial differences in tumour heterogeneity, including unique metabolism-driven subpopulations in Group 3 and matrix-producing subpopulations in SHH. To analyse tumour heterogeneity, we profiled individual tumour nodules at the cellular level in 3D MB hydrogel models, which recapitulate subgroup specific phenotypes, by single cell RNA sequencing (scRNAseq) and 3D OrbiTrap Secondary Ion Mass Spectrometry (3D OrbiSIMS) imaging. In addition to identifying known metabolites characteristic of MB, we observed intra- and internodular heterogeneity and identified subgroup-specific tumour subpopulations. We showed that extracellular matrix factors and adhesion pathways defined unique SHH subpopulations, and made up a distinct shell-like structure of sulphur-containing species, comprising a combination of small leucine-rich proteoglycans (SLRPs) including the collagen organiser lumican. In contrast, the Group 3 tumour model was characterized by multiple subpopulations with greatly enhanced oxidative phosphorylation and tricarboxylic acid (TCA) cycle activity. Extensive TCA cycle metabolite measurements revealed very high levels of succinate and fumarate with malate levels almost undetectable particularly in Group 3 tumour models. In patients, high fumarate levels (NMR spectroscopy) alongside activated stress response pathways and high Nuclear Factor Erythroid 2-Related Factor 2 (NRF2; gene expression analyses) were associated with poorer survival. Based on these findings we predicted and confirmed that NRF2 inhibition increased sensitivity to vincristine in a long-term 3D drug treatment assay of Group 3 MB. Thus, by combining scRNAseq and 3D OrbiSIMS in a relevant model system we were able to define MB subgroup heterogeneity at the single cell level and elucidate new druggable biomarkers for aggressive Group 3 and low-risk SHH MB.
Structural and cross-cultural validity of the Afrikaans for the Western Cape Disabilities of the Arm, Shoulder and Hand (DASH) questionnaire
Authors: de Klerk Susan , Jerosch-Herold Christina , Buchanan Helen , van Niekerk Lana
Published: January 2023

Background

The Disabilities of the Arm, Shoulder and Hand (DASH) questionnaire has been translated and cross-culturally adapted to Afrikaans for the Western Cape, within the public health service context of South Africa. The aim of this study was to evaluate structural validity, internal consistency, and cross-cultural validity/measurement invariance of this new translation to increase applicability and clinical utility in a public health service context.

Methods

During this cross-sectional study, exploratory factor analysis (EFA) was conducted with parallel analysis and oblimin rotation. Confirmatory factor analysis (CFA) and multiple group confirmatory factor analysis (MGCFA) to assess cross-cultural validity/measurement invariance, was employed to test model fit withX2goodness-of-fit statistic, root mean square error of approximation (RMSEA), standardized root mean square residual (SRMR) and comparative fit index (CFI). Internal consistency was calculated using Cronbach’s alpha.

Results

109 women and 110 men (n = 219) completed the Afrikaans for the Western Cape and the South African English DASH questionnaire, used during the analysis. Unidimensionality of the Afrikaans for the Western Cape DASH questionnaire was not supported in the 218 questionnaires eligible for inclusion in the analysis [X2(df) = 1799.10 (405);pvalue = < 0.01; RMSEA (90% CI) = 0.126 (0.120–0.132); SRMR = 0.09 and CFI = 0.984]. EFA revealed a two-factor structure with Eigenvalues exceeding one explaining 55% and 7% of the variance. The two-factor structure of the Afrikaans for the Western Cape DASH questionnaire was supported during CFA. Cronbach’s alpha revealed good internal consistency of both factors [factor 1 = 0.97 (0.96, 0.97) and factor 2 = 0.92 (0.90, 0.94)]. MGCFA conducted between 218 Afrikaans for the Western Cape DASH and 219 South African English DASH questionnaires (N = 437) revealed that the data supports configural, metric and scalar invariance models during initial model fit assessment. Subsequent hypotheses testing comparing the nested models revealed that scalar invariance holds.

Conclusion

The Afrikaans for the Western Cape DASH questionnaire revealed a two-factor structure with good internal consistency across the two factors and demonstrated measurement invariance with the South African English DASH questionnaire.
Black aortic valve and coronary arteries in liver transplantation patient: case report
Authors: Vancoillie S. , Roosen J. , Bourgain C. , Depraetere H.
Published: January 2023

Background

Black pigmentation of the aortic valve and coronary arteries has been reported to be caused by alkaptonuria or minocycline. In patients with liver transplantation it has been described only once, after exposure to minocycline. We report a second case of such pigmentation in a liver transplantation patient, for whom none of the known causes are an adequate explanation for this pigmentation.

Case presentation

Our 56-year-old male patient showed a complex history of two liver transplantations and sleeve gastrectomy, and had neither alkaptonuria nor a history of minocycline intake. He underwent an urgent coronary bypass grafting and aortic valve replacement because of an acute coronary syndrome based on three-vessel disease and progressive aortic valve stenosis. During this procedure, the aortic valve and coronary arteries had black pigmentation.

Conclusions

There are causes – other than alkaptonuria and minocycline – that can induce a black pigmentation of the aortic valve and coronary arteries. In patients with a history of liver transplantation, alteration in (dys)functional liver parenchyma or administration of substances related to the procedure of liver transplantation are possible causes. Identifying these is important because they are potentially harmful as they may induce degenerative changes and functional impairment of the aortic valve and coronary arteries. Further research is needed, but proves to be difficult due to the rare nature of this condition.
Proton pump inhibitors as treatment of laryngeal disorders among patients with gastroesophageal reflux disease: a single-arm (pre and post) quasi-experimental study
Authors: Ragaee Mahmoud Ali , Sedik Shaimaa Salah , Ahmed Hanan Abd El Rasheed Mohamed , Mahran Essam Eldeen Mohamed Osman , Roushdy Mohamed Mahmoud
Published: January 2023

Background

Laryngopharyngeal reflux is a silent cause of laryngeal disorders which was documented in the last decade by many studies. This study aimed to evaluate the prevalence of laryngeal reflux in GERD cases and to evaluate the effect of GERD treatment on these laryngeal disorders.

Methodology

In this single-arm (pre and post) quasi-experimental study, eighty patients with GERD were examined for laryngeal disorders by rigid laryngoscopy and assessed by the Reflux Finding Score (RFS). Complaints of patients were evaluated using the 9-item Reflux Symptom Index (RSI) questionnaire. Patients received medical treatment of GERD in the form of proton pump inhibitors (PPIs) only, and they were reassessed after 3 months for improvement in their symptoms and signs using RSI and RFS systems to compare patient complaints and findings in the laryngeal endoscopy pre- and post-treatment.

Results

Among 80 confirmed GERD cases, 70 (87.5%) suffered from laryngeal disorders. Throat clearing and postnasal drip were the most prevalent symptoms, and ventricular obliteration and erythema, vocal fold and diffuse laryngeal edema, and posterior commissure hypertrophy were the most detected signs. Significant improvement in both symptoms and signs after 3 months of PPI therapy was clearly observed. The resistance rate among our studied patients was reported to be 22.4%.

Conclusion

GERD can be considered as an inducer or cause of laryngeal disorders, with a significant direct proportional relationship between the severity of GERD and both the RSI and RFS. PPI greatly improves laryngeal disorders among GERD patients with a high response rate (77.6%).
Cognitive and Behavioral Development of 9-Year-Old Children After Maternal Cancer During Pregnancy: A Prospective Multicenter Cohort Study
Authors: Van Assche Indra A. , Huis in 't Veld Evangeline A. , Van Calsteren Kristel , van Gerwen Mathilde , Blommaert Jeroen , Cardonick Elyce , Halaska Michael J. , Fruscio Robert , Fumagalli Monica , Lemiere Jurgen , van Dijk-Lokkart Elisabeth M. , Fontana Camilla , van Tinteren Harm , De Ridder Jessie , van Grotel Martine , van den Heuvel-Eibrink Marry M. , Lagae Lieven , Amant Frédéric
Published: January 2023

Clinical trials frequently include multiple end points that mature at different times. The initial report, typically based on the primary end point, may be published when key planned co-primary or secondary analyses are not yet available. Clinical Trial Updates provide an opportunity to disseminate additional results from studies, published in JCO or elsewhere, for which the primary end point has already been reported.

This multicenter cohort study reports on the long-term effects of prenatal exposure to maternal cancer and its treatment on cognitive and behavioral outcomes in 9-year-old children. In total, 151 children (mean age, 9.3 years; range, 7.8-10.6 years) were assessed using a neurocognitive test battery and parent-report behavioral questionnaires. During pregnancy, 109 children (72.2%) were exposed to chemotherapy (only or in combination with other treatment modalities), 18 (11.9%) to surgery only, 16 (10.6%) to radiotherapy, one to trastuzumab, and 16 (10.6%) were not exposed to oncologic treatment. Mean cognitive and behavioral outcomes were within normal ranges. Gestational age at birth showed a positive association with Full Scale Intelligence Quotient (FSIQ), with the average FSIQ score increasing by 1.6 points for each week increase in gestational age (95% CI, 0.7 to 2.5; P < .001). No difference in FSIQ was found between treatment types (F[4,140] = 0.45, P = .776). In children prenatally exposed to chemotherapy, no associations were found between FSIQ and chemotherapeutic agent, exposure level, or timing during pregnancy. These results indicate a reassuring follow-up during the critical maturational period of late childhood, when complex functions develop and rely on the integrity of early brain development. However, associations were observed with preterm birth, maternal death, and maternal education.
Recent Advances in the Role of Autophagy in Endocrine-Dependent Tumors
Authors: Komarla Anvita , Dufresne Suzanne , Towers Christina G
Published: January 2023

Autophagy plays a complex role in several cancer types, including endocrine-dependent cancers, by fueling cellular metabolism and clearing damaged substrates. This conserved recycling process has a dual function across tumor types where it can be tumor suppressive at early stages but tumor promotional in established disease. This review highlights the controversial roles of autophagy in endocrine-dependent tumors regarding cancer initiation, tumorigenesis, metastasis, and treatment response. We summarize clinical trial results thus far and highlight the need for additional mechanistic, preclinical, and clinical studies in endocrine-dependent tumors, particularly in breast cancer and prostate cancer.
Recurrence of Primary Aldosteronism After Surgery in Aldosterone-producing Adenoma With KCNJ5 Gene Mutation
Authors: Aiga Ko , Kometani Mitsuhiro , Aono Daisuke , Yoneda Takashi
Published: January 2023

Primary aldosteronism (PA) is a major cause of secondary hypertension. Aldosterone-producing adenoma (APA) is a subtype of PA, and adrenalectomy is the gold-standard treatment. Recently, a high prevalence of the KCNJ5 gene mutation has been reported in APA, particularly in Japan. Herein, we present 2 extremely rare cases of PA recurrence more than 10 years after adrenalectomy for APA. In the first case, a 52-year-old woman was examined for hypertension 22 years after total adrenalectomy of the right adrenal gland. Recurrent PA was diagnosed based on high aldosterone-renin-ratio (ARR), identification of left adrenal gland tumor by computed tomography (CT), and a confirmatory test. In the second case, a 65-year-old man was examined for hypertension 17 years after total adrenalectomy of the left adrenal gland. He had maintained his blood pressure using medication since the onset of hypertension 4 years after the surgery. A year later, a high ARR was observed. PA recurrence was determined by a right adrenal gland tumor noted on CT and a confirmatory test. Somatic mutations in KCNJ5 were detected in the resected tissues in both cases. We recommend careful follow-ups after adrenalectomy in APA cases, especially in those with a KCNJ5 gene mutation.
Barriers to Screening: An Analysis of Factors Impacting Screening for Type 1 Diabetes Prevention Trials
Authors: Kinney Mara , You Lu , Sims Emily K , Wherrett Diane , Schatz Desmond , Lord Sandra , Krischer Jeffrey , Russell William E , Gottlieb Peter A , Libman Ingrid , Buckner Jane , DiMeglio Linda A , Herold Kevan C , Steck Andrea K
Published: January 2023

Context

Participants with stage 1 or 2 type 1 diabetes (T1D) qualify for prevention trials, but factors involved in screening for such trials are largely unknown.

Objective

To identify factors associated with screening for T1D prevention trials.

Methods

This study included TrialNet Pathway to Prevention participants who were eligible for a prevention trial: oral insulin (TN-07, TN-20), teplizumab (TN-10), abatacept (TN-18), and oral hydroxychloroquine (TN-22). Univariate and multivariate logistic regression models were used to examine participant, site, and study factors at the time of prevention trial accrual.

Results

Screening rates for trials were: 50% for TN-07 (584 screened/1172 eligible), 9% for TN-10 (106/1249), 24% for TN-18 (313/1285), 17% for TN-20 (113/667), and 28% for TN-22 (371/1336). Younger age and male sex were associated with higher screening rates for prevention trials overall and for oral therapies. Participants with an offspring with T1D showed lower rates of screening for all trials and oral drug trials compared with participants with other first-degree relatives as probands. Site factors, including larger monitoring volume and US site vs international site, were associated with higher prevention trial screening rates.

Conclusions

Clear differences exist between participants who screen for prevention trials and those who do not screen and between the research sites involved in prevention trial screening. Participant age, sex, and relationship to proband are significantly associated with prevention trial screening in addition to key site factors. Identifying these factors can facilitate strategic recruitment planning to support rapid and successful enrollment into prevention trials.
Multiple interactions of the dynein-2 complex with the IFT-B complex are required for effective intraflagellar transport
Authors: Hiyamizu Shunya , Qiu Hantian , Vuolo Laura , Stevenson Nicola L. , Shak Caroline , Heesom Kate J. , Hamada Yuki , Tsurumi Yuta , Chiba Shuhei , Katoh Yohei , Stephens David J. , Nakayama Kazuhisa
Published: January 2023

The dynein-2 complex must be transported anterogradely within cilia to then drive retrograde trafficking of the intraflagellar transport (IFT) machinery containing IFT-A and IFT-B complexes. Here, we screened for potential interactions between the dynein-2 and IFT-B complexes and found multiple interactions among the dynein-2 and IFT-B subunits. In particular, WDR60 (also known as DYNC2I1) and the DYNC2H1–DYNC2LI1 dimer from dynein-2, and IFT54 (also known as TRAF3IP1) and IFT57 from IFT-B contribute to the dynein-2–IFT-B interactions. WDR60 interacts with IFT54 via a conserved region N-terminal to its light chain-binding regions. Expression of the WDR60 constructs in WDR60-knockout (KO) cells revealed that N-terminal truncation mutants lacking the IFT54-binding site fail to rescue abnormal phenotypes of WDR60-KO cells, such as aberrant accumulation of the IFT machinery around the ciliary tip and on the distal side of the transition zone. However, a WDR60 construct specifically lacking just the IFT54-binding site substantially restored the ciliary defects. In line with the current docking model of dynein-2 with the anterograde IFT trains, these results indicate that extensive interactions involving multiple subunits from the dynein-2 and IFT-B complexes participate in their connection.
Microtubule–mitochondrial attachment facilitates cell division symmetry and mitochondrial partitioning in fission yeast
Authors: Chacko Leeba Ann , Mikus Felix , Ariotti Nicholas , Dey Gautam , Ananthanarayanan Vaishnavi
Published: January 2023

Association with microtubules inhibits the fission of mitochondria in Schizosaccharomyces pombe. Here, we show that this attachment of mitochondria to microtubules is an important cell-intrinsic factor in determining cell division symmetry. By comparing mutant cells that exhibited enhanced attachment and no attachment of mitochondria to microtubules (Dnm1Δ and Mmb1Δ, respectively), we show that microtubules in these mutants displayed aberrant dynamics compared to wild-type cells, which resulted in errors in nuclear positioning. This translated to cell division asymmetry in a significant proportion of both Dnm1Δ and Mmb1Δ cells. Asymmetric division in Dnm1Δ and Mmb1Δ cells resulted in unequal distribution of mitochondria, with the daughter cell that received more mitochondria growing faster than the other daughter cell. Taken together, we show the existence of homeostatic feedback controls between mitochondria and microtubules in fission yeast, which directly influence mitochondrial partitioning and, thereby, cell growth.

This article has an associated First Person interview with the first author of the paper.
Photon-counting detector coronary CT angiography: impact of virtual monoenergetic imaging and iterative reconstruction on image quality
Authors: Sartoretti Thomas , McDermott Michael , Mergen Victor , Euler André , Schmidt Bernhard , Jost Gregor , Wildberger Joachim E , Alkadhi Hatem
Published: January 2023

Objectives:

To assess the impact of low kilo-electronvolt (keV) virtual monoenergetic image (VMI) energies and iterative reconstruction on image quality of clinical photon-counting detector coronary CT angiography (CCTA).

Methods:

CCTA with PCD-CT (prospective ECG-triggering, 120 kVp, automatic tube current modulation) was performed in a high-end cardiovascular phantom with dynamic flow, pulsatile heart motion, and including different calcified plaques with various stenosis grades and in 10 consecutive patients. VMI at 40,50,60 and 70 keV were reconstructed without (QIR-off) and with all quantum iterative reconstruction (QIR) levels (QIR-1 to 4). In the phantom, noise power spectrum, vessel attenuation, contrast-to-noise-ratio (CNR), and vessel sharpness were measured. Two readers graded stenoses in the phantom and graded overall image quality, subjective noise, vessel sharpness, vascular contrast, and coronary artery plaque delineation on 5-point Likert scales in patients.

Results:

In the phantom, noise texture was only slightly affected by keV and QIR while noise increased by 69% from 70 keV QIR-4 to 40 keV QIR-off. Reconstructions at 40 keV QIR-4 exhibited the highest CNR (46.1 ± 1.8), vessel sharpness (425 ± 42 ∆HU/mm), and vessel attenuation (1098 ± 14 HU). Stenosis measurements were not affected by keV or QIR level (p > 0.12) with an average error of 3%/6% for reader 1/reader 2, respectively. In patients, across all subjective categories and both readers, 40 keV QIR-3 and QIR-4 images received the best scores (p < 0.001).

Conclusion:

Forty keV VMI with QIR-4 significantly improved image quality of CCTA with PCD-CT.

Advances in knowledge:

PCD-CT at 40 keV and QIR-4 improves image quality of CCTA.
Measuring reader fatigue in the interpretation of screening digital breast tomosynthesis (DBT)
Authors: Chen Yan , Sudin Ellhia S , Partridge George JW , Taib Adnan G , Darker Iain T , Phillips Peter , James Jonathan J , Satchithananda Keshthra , Sharma Nisha , Michell Michael J
Published: January 2023

Objectives:

The interpretation of digital breast tomosynthesis (DBT) screening examinations is a complex task for an already overstretched workforce which has the potential to increase pressure on readers leading to fatigue and patient safety issues. Studies in non-medical and medical settings have suggested that changes in blink characteristics can reflect fatigue. The purpose of this study is to investigate the use of blink characteristics as an objective marker of fatigue in readers interpreting DBT breast screening examinations.

Methods:

Twenty-six DBT readers involved in the UK PROSPECTS trial interpreted a test set of 40 DBT cases while being observed by an eye tracking device from November 2019 to February 2021. Raw data from the eye tracker were collected and automated processing software was used to produce eye blinking characteristics data which were analysed using multiple linear regression statistical models.

Results:

Of the 26 DBT readers recruited, eye tracking data from 23 participants were analysed due to missing data rendering 3 participants’ data uninterpretable. The mean reading time per DBT case was 2.81 min. There was a statistically significant increase in blinking duration of 0.38 ms/case as the reading session progressed (p < 0.0001). This was the result of a significant decrease in the number of ultra-short blinks lasting ≤50 ms (p = 0.0005) and a significant increase in longer blinks lasting 51–100 ms (p = 0.008).

Conclusion

Changes in blinking characteristics could serve as objective measures of reader fatigue and may prove useful in the development of DBT reading protocols.

Advances in knowledge:

Blink characteristics can be used as an objective measure of fatigue; however there is limited evidence of their use in radiological settings. Our study suggests that changes in blink duration and frequency could be used to monitor fatigue in DBT reading sessions.
Vaccine Hesitancy in Japan: From a Perspective on Medical Uncertainty and Trans-Scientific Theory
Authors: Hosoda Miwako
Published: January 2023

The development and dissemination of vaccines has made immunization possible and has led to the successful control and eradication of various infectious diseases in many parts of the world. However, even when vaccines that are said to be "effective" are offered, a certain number of people do not receive them, and this has become a problem known as "vaccine hesitancy”. ItThe reason of “Vaccine hesitancy” is pointed out that there is not only because of the risk of contracting infectious diseases if they are not vaccinated, but also because of the lack of a collective immunity system. Vaccines are an effective means of acquiring immunity, but no matter how highly effective vaccines are developed, if the vaccination rate does not exceed a certain number, “herd immunity”, which means that the risk of person-to-person transmission is reduced when a significant portion of the population becomes immune to, cannot be acquired. Therefore, how to increase the vaccination rate of the population is a major public health challenge. This paper reviews previous studies on "vaccine hesitancy" in Japan and analyzes people's hesitancy in terms of negative "rumors" about vaccines, risk perception of vaccine side effects, and sense of burden when receiving vaccinations. Then, the author will examine that the background of "vaccine hesitancy" is not only distrust of vaccines and risk perception of side effects, but also distrust of those who provide and promote vaccinations, such as medical professionals, government, and public administration. By using medical uncertainty which shows there are many unknowns and uncertainties in medicine and trans-scientific theory which indicates there are areas that science cannot answer this paper argues that the problem of "vaccine hesitancy" can be reduced if medical professionals and governments show sincere empathy and attitude toward victims of adverse vaccine reactions and those who hesitate to vaccinate.
At the nexus of ludology and narratology: Advances in reality-based story-driven games
Authors: Chen Lei , Dowling David , Goetz Christopher
Published: January 2023

Story-driven games are growing in popularity across a wide range of genres. However, the narrative potential of video games is still being debated, particularly in light of the so-called tension between gameplay and storytelling. This study proposes that rules and game mechanics perform narrative semiotic functions, offering a ludic grammar of interactive storytelling. Case studies of four representative games show through exploratory player action shaped by rules, the medium of video games can generate meanings that traditional media cannot, thereby better achieving their narrative goals.
A Theory-Driven Deep Learning Method for Voice Chat–Based Customer Response Prediction
Authors: Chen Gang , Xiao Shuaiyong , Zhang Chenghong , Zhao Huimin
Published: January 2023

A Theory-Driven Deep Learning Method for Voice Chat–Based Customer Response Prediction

In this study, we target the task of voice chat–based customer response prediction in an emerging online interaction–based commercial mode, the invite-online-and-experience-in-store mode. Prior research shows that satisfaction, which can be revealed by the discrepancy between prior expectation and actual experience, is a key factor to disentangle customers’ purchase intention, whereas black box deep learning methods empirically promise us with advantageous capabilities in dealing with complex voice data, for example, text and audio information incorporated in voice chat. To this end, we propose a theory-driven deep learning method that enables us to (1) learn customers’ personalized product preferences and dynamic satisfaction in the absence of their profile information, (2) model customers’ actual experiences based on multiview voice chat information in an interlaced way, and (3) enhance the customer response prediction performance of a black box deep learning model with theory-driven dynamic satisfaction. Empirical evaluation results demonstrate the advantageous prediction performance of our proposed method over state-of-the-art deep learning alternatives. Investigation of cumulative satisfaction reveals the collaborative predictive roles of theory-driven dynamic satisfaction and deep representation features for customer response prediction. Explanatory analysis further renders insights into customers’ personalized preferences and dynamic satisfaction for key product attributes.
Two-Stage Stochastic Matching and Pricing with Applications to Ride Hailing
Authors: Feng Yiding , Niazadeh Rad , Saberi Amin
Published: January 2023

Two-Stage Matching and Pricing in Ride-Hailing Platforms

Matching and pricing are two critical levers in two-sided marketplaces to connect demand and supply. The platform can produce more efficient matching and pricing decisions by batching the demand requests. We initiate the study of the two-stage stochastic matching problem with or without pricing to enable the platform to make improved decisions in a batch with an eye toward the imminent future demand requests. This problem is motivated in part by applications in online marketplaces, such as ride-hailing platforms. We design online competitive algorithms for vertex-weighted (or unweighted) two-stage stochastic matching for maximizing supply efficiency and two-stage joint matching and pricing for maximizing market efficiency. Using various techniques, such as introducing convex programming–based matching and graph decompositions, submodular maximization, and factor-revealing linear programs, we obtain either optimal competitive or improved approximation algorithms compared with naïve solutions. We enrich our theoretical study by data-driven numerical simulations using DiDi’s ride-sharing data sets.
Thrombolysis Through Punctured Coronary Balloon and 014 Wire Mediated Guitaring After IVC Filter Insertion in Iliofemoral Deep Vein Thrombosis
Authors: Arokiaraj Mark Christopher
Published: January 2023

This is a report of a patient who presented with iliofemoral deep vein thrombosis, and was treated with pharmaco-invasive and pharmaco- mechanic methods using coronary balloon and mechanical disruption of clots with coronary 014 wire. A 65-year-old male presented with acute deep vein thrombosis extending from the left common iliac to the popliteal vein. The patient had signi cant swelling and pain. An inferior vena cava (IVC) lter was inserted, and catheter-directed thrombolysis was planned. A technical dif culty was encountered entering the proximal common iliac vein. Hence, a 014 balance middle weight (BMW) wire with coronary balloon support was introduced through a Cordis® 6 F. diagnostic catheter, and the 014 wire was advanced with guitaring technique up to the femoral vein. Thereafter, the coronary balloon was ruptured at high pressure, and thrombolysis using streptokinase was performed along the balloon tract up to the ostium of the common iliac vein. The patient symptomatically improved signi cantly and is currently on anticoagulants. Follow-up after one year showed minimal residual oedema, and less pain in the ankle region. Pharmaco-mechanical treatment is possible using coronary balloon dilatations and thrombolysis through a ruptured coronary balloon. Mechanical disruption of clots to some extent is feasible using 014 wire by a guitaring technique.
On your marks, get set, pause: what care home teams should consider before partnering with a trial research group
Authors: Spilsbury Karen , Peryer Guy , Devi Reena , Haunch Kirsty , Jordao Magda , Thompson Carl , Goodman Claire
Published: January 2023

Research has the potential to inform and enhance the care and experiences of people living and working in care homes. While there is a growing interest in research relevant for care homes, there is also a need to ensure that staff, residents and their families and friends are supported when considering taking part in research; particularly in a type of research called a ‘trial’. Trials are a type of research study that can help guide decisions about the best treatment, care and support for both residents (and their families and friends) and care home staff. While potentially important, trials are demanding for both care homes and trial research teams. Before agreeing to support a trial, there are questions a care home manager, staff, residents and their families and friends can consider. These questions are outlined in this article, and the answers to these will help a care home to determine whether they have the capacity, readiness and relationships to support a trial. By taking the time to ‘pause’ and ask ‘are we ready?’, care teams can support both care and science.
GRU Based Time Series Forecast of Oil Temperature in Power Transformer
Authors: Chen Haomin , Meng Lingwen , Xi Yu , Xin Mingyong , Yu Siwu , Chen Guangqin , Chen Yumin
Published: January 2023

With the continuous progress of the society, the demand for electrical power is urgent. The transformer plays an important role in the power energy transmission. The oil temperature inside transformer effectively could reflect working condition of the transformer, which makes it necessary to monitor and forecast the oil temperature to monitor the operating status of the power transformer. However, the oil temperature time series data generated by the power transformer has the characteristics of being complex and nonlinear. In recent years, long and short time memory networks (LSTM) are often used to predict transformer oil temperature. Gated recurrent unit (GRU) is a new version for LSTM. In the structure of GRU, there exist two gates, which are updating gate and resetting gate, respectively. Compared with LSTM network, The structure of GRU is simpler and its effect is better. A novel predicting method for transformer oil temperature is proposed based on time series theory and GRU in this paper, which is verified on the dataset of the oil temperature of the transformers in the two regions. The experimental results are compared with traditional time series prediction models to demonstrate that the proposed method is effective and feasible.
Ethnobotanical study of medicinal plants used to treat human diseases in Gura Damole District, Bale Zone, Southeast Ethiopia
Authors: Assefa Behailu , Megersa Moa , Jima Tilahun Tolossa
Published: January 2023

Abstract. Assefa B, Megersa M, Jima TT. 2021. Ethnobotanical study of medicinal plants used to treat human diseases in Gura Damole District, Bale Zone, Southeast Ethiopia. Asian J Ethnobiol 4: 42-52. Many countries, including Ethiopia, use medicinal plants for their primary health care system. Plants have been used as a source of medicine to treat human diseases in Ethiopia. This study aimed to document medicinal plants to treat various human diseases in southeast Ethiopia's, the Gura Damole District of Oromia Regional State. Ethnobotanical survey of medicinal plants was carried out from August 05 to November 06, 2019. A total of 90 informants were selected to collect ethnobotanical information from 6 kebeles. Ethnobotanical data were collected using semi-structured interviews, focus group discussions with informants, and field observation. Various ethnobotanical ranking indices were used to analyze the importance of some plant species. A total of 30 medicinal plants belonging to 21 families were identified. These medicinal plants comprised shrubs (36.6%), trees (26.6%), herbs (23.3%), and lianas (13.3%). The plant families with the highest medicinal plants in the study area used for various diseases treatment were Asteraceae and Solanaceae (3 species each). Leaves (46.7%) were the dominant plant part used to prepare remedies, followed by roots (36.7%). Powdering (50%) and oral route of administration (59%) were commonly mentioned methods of preparation and administration, respectively. Carissa spinarum was the most preferred medicinal plant to treat evil eyes and is also ranked highest as the preferable medicinal plant for various purposes. Although the current study revealed the existence of indigenous knowledge of medicinal plants to treat human diseases, agricultural expansion became the primary threat to medicinal plants. Hence, different conservation methods should be applied to conserve those mostly preferred and frequently used medicinal plants for various purposes.
Floristic and ethnic perspective on wild forest plant species of Nongkhyllem Reserve Forest, Nongpoh, Meghalaya, India
Authors: PARKASH VIPIN
Published: January 2023

Abstract. Parkash V. 2021. Floristic and ethnic perspective on wild forest plant species of Nongkhyllem Reserve Forest, Nongpoh, Meghalaya, India. Asian J Ethnobiol 4: 93-105. This paper elucidates the indigenous traditional knowledge of wild plants usage by Khasi people (ethnic group) of Nongkhyllem Reserve Forest, Nongpoh, Meghalaya, India. About 117 different wild forest plant species belonging to 63 families were collected and enumerated for their traditional usage. Most of the plant parts utilized were of herbs (44) followed by trees (32) and shrubs (28). The climbers (7) and scrubs (6) have been utilized least in traditional usage by Khasi people in Nongkhyllem Reserve Forest. It is interesting to mention that due to overexploitation, some plant species, i.e., Abroma augusta L., Phlogacanthus thyrsiflorus (Roxb.), Puereria tuberosa L., Eryngium foetidum L., Nees, Smilax aspera L. and Houttuynia cordata Thunb. were utilized in colossal quantity, and these plant species are nearing their threshold and hence, are under threat. Only two plants of A. augusta L. were found around forest edge areas of reserve forest, so there is a need of the hour to conserve this plant species in situ and ex-situ conditions. This study emphasizes research potentials and the need to document traditional knowledge about wild forest plant species utilization to benefit society and humankind through scientific intervention.