bluuebunny/crossref_metadata_2025 huggingface dataset:
Combined Influence of Low-Grade Metakaolins and Natural Zeolite on Compressive Strength and Heavy Metal Adsorption of Geopolymers
Authors: Sudagar Alcina Johnson , Andrejkovičová Slávka , Rocha Fernando , Patinha Carla , Soares Maria R. , Velosa Ana Luísa , Silva Eduardo Ferreira da
Published: May 2021

Metakaolins (MKs) prepared from low-grade kaolins located in the Alvarães (A) and Barqueiros (B) regions of Portugal were used as the aluminosilicate source to compare their effect on the compressive strength and heavy metal adsorption of geopolymers. Natural zeolite, an inexpensive, efficient adsorbent, was used as an additive in formulations to enhance geopolymers’ adsorption capacities and reduce MK utilization’s environmental footprint. Geopolymers were synthesized with the replacement of MK by zeolite up to 75 wt.% (A25, B25—25% MK 75% zeolite; A50, B50—50% MK 50% zeolite; A75, B75—75% MK 25% zeolite; A100, B100—100% MK). The molar ratios of SiO2/Al2O3 and Na2O/Al2O3 were kept at 1 to reduce the sodium silicate and sodium hydroxide environmental impact. Geopolymers’ crystallography was identified using X-ray diffraction analysis. The surface morphology was observed by scanning electron microscopy to understand the effect of zeolite incorporation. Chemical analysis using X-ray fluorescence spectroscopy and energy dispersive X-ray spectroscopy yielded information about the geopolymers’ Si/Al ratio. Compressive strength values of geopolymers obtained after 1, 14, and 28 days of curing indicate high strengths of geopolymers with 100% MK (A100—15.4 MPa; B100—32.46 MPa). Therefore, zeolite did not aid in the improvement of the compressive strength of both MK-based geopolymers. The heavy metal (Cd2+, Cr3+, Cu2+, Pb2+, and Zn2+) adsorption tests exhibit relatively higher adsorption capacities of Barqueiros MK-based geopolymers for all the heavy metals except Cd2+. Moreover, zeolite positively influenced divalent cations’ adsorption on the geopolymers produced from Barqueiros MK as B75 exhibits the highest adsorption capacities, but such an influence is not observed for Alvarães MK-based geopolymers. The general trend of adsorption of the heavy metals of both MK-based geopolymers is Pb2+ > Cd2+ > Cu2+ > Zn2+ > Cr3+ when fitted by the Langmuir isotherm adsorption model. The MK and zeolite characteristics influence geopolymers’ structure, strength, and adsorption capacities.
Combining Isotope Dilution and Standard Addition—Elemental Analysis in Complex Samples
Authors: Brauckmann Christine , Pramann Axel , Rienitz Olaf , Schulze Alexander , Phukphatthanachai Pranee , Vogl Jochen
Published: May 2021

A new method combining isotope dilution mass spectrometry (IDMS) and standard addition has been developed to determine the mass fractions w of different elements in complex matrices: (a) silicon in aqueous tetramethylammonium hydroxide (TMAH), (b) sulfur in biodiesel fuel, and (c) iron bound to transferrin in human serum. All measurements were carried out using inductively coupled plasma mass spectrometry (ICP–MS). The method requires the gravimetric preparation of several blends (bi)—each consisting of roughly the same masses (mx,i) of the sample solution (x) and my,i of a spike solution (y) plus different masses (mz,i) of a reference solution (z). Only these masses and the isotope ratios (Rb,i) in the blends and reference and spike solutions have to be measured. The derivation of the underlying equations based on linear regression is presented and compared to a related concept reported by Pagliano and Meija. The uncertainties achievable, e.g., in the case of the Si blank in extremely pure TMAH of urel (w(Si)) = 90% (linear regression method, this work) and urel (w(Si)) = 150% (the method reported by Pagliano and Meija) seem to suggest better applicability of the new method in practical use due to the higher robustness of regression analysis.
Choline Chloride–Lactic Acid-Based NADES As an Extraction Medium in a Response Surface Methodology-Optimized Method for the Extraction of Phenolic Compounds from Hazelnut Skin
Authors: Fanali Chiara , Gallo Valeria , Della Posta Susanna , Dugo Laura , Mazzeo Leone , Cocchi Marco , Piemonte Vincenzo , De Gara Laura
Published: May 2021

Deep eutectic solvents (DESs) are promising green solvents for the extraction of compounds from food byproducts. Hazelnut (Corylus avellana L.) is one of the most commonly cultivated tree nuts worldwide. The skin represents one of the major byproducts of the hazelnut industry and accounts for 2.5% of the total hazelnut kernel weight. It is a rich source of phenolic compounds like flavan-3-ols, flavonols, dihydrochalcones, and phenolic acids. In this work, fifteen DESs based on choline chloride and betaine, with different compositions, were studied in order to test their phenolic compounds extraction efficiency through the determination of their total concentration via Folin–Ciocalteu assay. A qualitative analysis of extracted phenolic compounds was assessed by HPLC with UV and MS detection. Using the DES with the best extraction efficiency, a new ultrasound-assisted solid liquid extraction (UA-SLE) method was optimized though the response surface methodology (RSM), taking into account some extraction parameters. Efficient recovery of extracted phenolic compounds was achieved using a 35% water solution of choline chloride and lactic acid (molar ratio 1:2) as an extraction solvent, working at 80 °C and with a solid-to-solvent ratio of 1:25 gmL−1. The optimized conditions made it possible to recover 39% more phenolic compounds compared to a classic organic solvent.
Liquid Crystals Based on the N-Phenylpyridinium Cation—Mesomorphism and the Effect of the Anion
Authors: Herod Jordan D. , Bruce Duncan W.
Published: May 2021

Families of symmetric, ionic, tetracatenar mesogens are described based on a rigid, N-phenylpyridinium core, prepared as their triflimide, octyl sulfate and dodecyl sulfate salts for a range of terminal chain lengths. The mesomorphism of the individual series is described before a comparison is drawn between the different families and then more broadly with (i) neutral tetracatenar materials and (ii) related bis(3,4-dialkoxystilbazole)silver(I) salts. For the octyl and dodecyl sulfates and the related triflates reported earlier, a SmA phase is seen at shorter chain lengths, giving way to a Colh phase as the terminal chain lengthens. For the alkyl sulfate salts, an intermediate cubic phase is also seen and the terminal chain length required to cause the change from lamellar to columnar mesophase depends on the anion. Furthermore, there is an unexpected and sometime very large mesophase stabilisation seen on entering the columnar phase. All of the triflimide salts show a rectangular columnar (ribbon) phase.
Olive-Derived Triterpenes Suppress SARS COV-2 Main Protease: A Promising Scaffold for Future Therapeutics
Authors: Alhadrami Hani A. , Sayed Ahmed M. , Sharif Ahmed M. , Azhar Esam I. , Rateb Mostafa E.
Published: May 2021

SARS CoV-2 pandemic is still considered a global health disaster, and newly emerged variants keep growing. A number of promising vaccines have been recently developed as a protective measure; however, cost-effective treatments are also of great importance to support this critical situation. Previously, betulinic acid has shown promising antiviral activity against SARS CoV via targeting its main protease. Herein, we investigated the inhibitory potential of this compound together with three other triterpene congeners (i.e., ursolic acid, maslinic acid, and betulin) derived from olive leaves against the viral main protease (Mpro) of the currently widespread SARS CoV-2. Interestingly, betulinic, ursolic, and maslinic acids showed significant inhibitory activity (IC50 = 3.22–14.55 µM), while betulin was far less active (IC50 = 89.67 µM). A comprehensive in-silico analysis (i.e., ensemble docking, molecular dynamic simulation, and binding-free energy calculation) was then performed to describe the binding mode of these compounds with the enzyme catalytic active site and determine the main essential structural features required for their inhibitory activity. Results presented in this communication indicated that this class of compounds could be considered as a promising lead scaffold for developing cost-effective anti-SARS CoV-2 therapeutics.
Molecular Regulation of Betulinic Acid on α3β4 Nicotinic Acetylcholine Receptors
Authors: Lee Shinhui , Jung Woog , Eom Sanung , Yeom Hye Duck , Park Heui-Dong , Lee Junho H.
Published: May 2021

Betulinic acid (BA) is a major constituent of Zizyphus seeds that have been long used as therapeutic agents for sleep-related issues in Asia. BA is a pentacyclic triterpenoid. It also possesses various anti-cancer and anti-inflammatory effects. Current commercially available sleep aids typically use GABAergic regulation, for which many studies are being actively conducted. However, few studies have focused on acetylcholine receptors that regulate wakefulness. In this study, we utilized BA as an antagonist of α3β4 nicotinic acetylcholine receptors (α3β4 nAChRs) known to regulate rapid-eye-movement (REM) sleep and wakefulness. Effects of BA on α3β4 nAChRs were concentration-dependent, reversible, voltage-independent, and non-competitive. Site-directed mutagenesis and molecular-docking studies confirmed the binding of BA at the molecular level and showed that the α3 subunit L257 and the β4 subunit I263 residues affected BA binding. These data demonstrate that BA can bind to a binding site different from the site for the receptor’s ligand, acetylcholine (ACh). This suggests that BA may be an effective antagonist that is unaffected by large amounts of ACh released during wakefulness and REM sleep. Based on the above experimental results, BA is likely to be a therapeutically useful sleep aid and sedative.
β-Boswellic Acid Inhibits RANKL-Induced Osteoclast Differentiation and Function by Attenuating NF-κB and Btk-PLCγ2 Signaling Pathways
Authors: Park Gyeong Do , Cheon Yoon-Hee , Eun So Young , Lee Chang Hoon , Lee Myeung Su , Kim Ju-Young , Cho Hae Joong
Published: May 2021

Osteoporosis is a systemic metabolic bone disorder that is caused by an imbalance in the functions of osteoclasts and osteoblasts and is characterized by excessive bone resorption by osteoclasts. Targeting osteoclast differentiation and bone resorption is considered a good fundamental solution for overcoming bone diseases. β-boswellic acid (βBA) is a natural compound found in Boswellia serrata, which is an active ingredient with anti-inflammatory, anti-rheumatic, and anti-cancer effects. Here, we explored the anti-resorptive effect of βBA on osteoclastogenesis. βBA significantly inhibited the formation of tartrate-resistant acid phosphatase-positive osteoclasts induced by receptor activator of nuclear factor-B ligand (RANKL) and suppressed bone resorption without any cytotoxicity. Interestingly, βBA significantly inhibited the phosphorylation of IκB, Btk, and PLCγ2 and the degradation of IκB. Additionally, βBA strongly inhibited the mRNA and protein expression of c-Fos and NFATc1 induced by RANKL and subsequently attenuated the expression of osteoclast marker genes, such as OC-STAMP, DC-STAMP, β3-integrin, MMP9, ATP6v0d2, and CtsK. These results suggest that βBA is a potential therapeutic candidate for the treatment of excessive osteoclast-induced bone diseases such as osteoporosis.
Comprehensive Two-Dimensional Gas Chromatography–Mass Spectrometry Analysis of Exhaled Breath Compounds after Whole Grain Diets
Authors: Raninen Kaisa , Nenonen Ringa , Järvelä-Reijonen Elina , Poutanen Kaisa , Mykkänen Hannu , Raatikainen Olavi
Published: May 2021

Exhaled breath is a potential noninvasive matrix to give new information about metabolic effects of diets. In this pilot study, non-targeted analysis of exhaled breath volatile organic compounds (VOCs) was made by comprehensive two-dimensional gas chromatography–mass spectrometry (GCxGC-MS) to explore compounds relating to whole grain (WG) diets. Nine healthy subjects participated in the dietary intervention with parallel crossover design, consisting of two high-fiber diets containing whole grain rye bread (WGR) or whole grain wheat bread (WGW) and 1-week control diets with refined wheat bread (WW) before both diet periods. Large interindividual differences were detected in the VOC composition. About 260 VOCs were detected from exhaled breath samples, in which 40 of the compounds were present in more than half of the samples. Various derivatives of benzoic acid and phenolic compounds, as well as some furanones existed in exhaled breath samples only after the WG diets, making them interesting compounds to study further.
Supramolecular Control of Singlet Oxygen Generation
Authors: Kashyap Akshay , Ramasamy Elamparuthi , Ramalingam Vijayakumar , Pattabiraman Mahesh
Published: May 2021

Singlet oxygen (1O2) is the excited state electronic isomer and a reactive form of molecular oxygen, which is most efficiently produced through the photosensitized excitation of ambient triplet oxygen. Photochemical singlet oxygen generation (SOG) has received tremendous attention historically, both for its practical application as well as for the fundamental aspects of its reactivity. Applications of singlet oxygen in medicine, wastewater treatment, microbial disinfection, and synthetic chemistry are the direct results of active past research into this reaction. Such advancements were achieved through design factors focused predominantly on the photosensitizer (PS), whose photoactivity is relegated to self-regulated structure and energetics in ground and excited states. However, the relatively new supramolecular approach of dictating molecular structure through non-bonding interactions has allowed photochemists to render otherwise inactive or less effective PSs as efficient 1O2 generators. This concise and first of its kind review aims to compile progress in SOG research achieved through supramolecular photochemistry in an effort to serve as a reference for future research in this direction. The aim of this review is to highlight the value in the supramolecular photochemistry approach to tapping the unexploited technological potential within this historic reaction.
Representations, Affordances, and Interactive Systems
Authors: Rowe Robert
Published: May 2021

The history of algorithmic composition using a digital computer has undergone many representations—data structures that encode some aspects of the outside world, or processes and entities within the program itself. Parallel histories in cognitive science and artificial intelligence have (of necessity) confronted their own notions of representations, including the ecological perception view of J.J. Gibson, who claims that mental representations are redundant to the affordances apparent in the world, its objects, and their relations. This review tracks these parallel histories and how the orientations and designs of multimodal interactive systems give rise to their own affordances: the representations and models used expose parameters and controls to a creator that determine how a system can be used and, thus, what it can mean.
Atomistic Band-Structure Computation for Investigating Coulomb Dephasing and Impurity Scattering Rates of Electrons in Graphene
Authors: Do Thi-Nga , Huang Danhong , Shih Po-Hsin , Lin Hsin , Gumbs Godfrey
Published: May 2021

In this paper, by introducing a generalized quantum-kinetic model which is coupled self-consistently with Maxwell and Boltzmann transport equations, we elucidate the significance of using input from first-principles band-structure computations for an accurate description of ultra-fast dephasing and scattering dynamics of electrons in graphene. In particular, we start with the tight-binding model (TBM) for calculating band structures of solid covalent crystals based on localized Wannier orbital functions, where the employed hopping integrals in TBM have been parameterized for various covalent bonds. After that, the general TBM formalism has been applied to graphene to obtain both band structures and wave functions of electrons beyond the regime of effective low-energy theory. As a specific example, these calculated eigenvalues and eigen vectors have been further utilized to compute the Bloch-function form factors and intrinsic Coulomb diagonal-dephasing rates for induced optical coherence of electron-hole pairs in spectral and polarization functions, as well as the energy-relaxation time from extrinsic impurity scattering of electrons for non-equilibrium occupation in band transport.
Imaging Thermoelectric Properties at the Nanoscale
Authors: Grauby Stéphane , Ben Amor Aymen , Hallais Géraldine , Vincent Laetitia , Dilhaire Stefan
Published: May 2021

Based on our previous experimental AFM set-up specially designed for thermal conductivity measurements at the nanoscale, we have developed and validated a prototype which offers two major advantages. On the one hand, we can simultaneously detect various voltages, providing, at the same time, both thermal and electrical properties (thermal conductivity, electrical conductivity and Seebeck coefficient). On the other hand, the AFM approach enables sufficient spatial resolution to produce images of nanostructures such as nanowires (NWs). After a software and hardware validation, we show the consistency of the signals measured on a gold layer on a silicon substrate. Finally, we demonstrate that the imaging of Ge NWs can be achieved with the possibility to extract physical properties such as electrical conductivity and Seebeck coefficient, paving the way to a quantitative estimation of the figure of merit of nanostructures.
Anisotropic Generation and Detection of Coherent Ag Phonons in Black Phosphorus
Authors: Lee Seong-Yeon , Yee Ki-Ju
Published: May 2021

Black phosphorus (BP) has attracted great attention due to its layer-tuned direct bandgap, in-plane anisotropic properties, and novel optoelectronic applications. In this work, the anisotropic characteristics of BP crystal in terms of the Raman tensor and birefringence are studied by investigating polarization dependence in both the generation and detection of Ag mode coherent phonons. While the generated coherent phonons exhibit the typical linear dichroism of BP crystal, the detection process is found here to be influenced by anisotropic multiple thin film interference, showing wavelength and sample thickness sensitive behaviors. We additionally find that the Ag1 and Ag2 optical phonons decay into lower frequency acoustic phonons through the temperature-dependent anharmonic process.
Gas Porosimetry by Gas Adsorption as an Efficient Tool for the Assessment of the Shaping Effect in Commercial Zeolites
Authors: Orsikowsky-Sanchez Alejandro , Franke Christine , Sachse Alexander , Ferrage Eric , Petit Sabine , Brunet Julien , Plantier Frédéric , Miqueu Christelle
Published: May 2021

A set of three commercial zeolites (13X, 5A, and 4A) of two distinct shapes have been characterized: (i) pure zeolite powders and (ii) extruded spherical beads composed of pure zeolite powders and an unknown amount of binder used during their preparation process. The coupling of gas porosimetry experiments using argon at 87 K and CO2 at 273 K allowed determining both the amount of the binder and its effect on adsorption properties. It was evidenced that the beads contain approximately 25 wt% of binder. Moreover, from CO2 adsorption experiments at 273 K, it could be inferred that the binder present in both 13X and 5A zeolites does not interact with the probe molecule. However, for the 4A zeolite, pore filling pressures were shifted and strong interaction with CO2 was observed leading to irreversible adsorption of the probe. These results have been compared to XRD, IR spectroscopy, and ICP-AES analysis. The effect of the binder in shaped zeolite bodies can thus have a crucial impact on applications in adsorption and catalysis.
Iodine Intake Estimated by 24 h Urine Collection in the Italian Adult Population: 2008–2012 Survey
Authors: Iacone Roberto , Iaccarino Idelson Paola , Formisano Pietro , Russo Ornella , Lo Noce Cinzia , Donfrancesco Chiara , Macchia Paolo Emidio , Palmieri Luigi , Galeone Daniela , di Lenarda Andrea , Giampaoli Simona , Strazzullo Pasquale
Published: May 2021

Monitoring the population iodine status is essential for iodine deficiency eradication. This study assessed the average dietary iodine intake and the iodine status of a random sample of the Italian general adult population. The study population included 2378 adults aged 35–79 years (1229 men and 1149 women) from all 20 Italian regions, participating in the Osservatorio Epidemiologico Cardiovascolare/Health Examination Survey 2008–2012 (OEC/HES), and were examined for iodine intake in the framework of the MINISAL-GIRCSI Programme. Dietary iodine intake was assessed by the measurement of 24 h urinary iodine excretion. The median daily iodine intake of the whole population was lower (96 µg/d, interquartile range 51–165) than the daily adequate iodine intake according to both EFSA and WHO recommendation (150 µg/d), with a significantly lower value among women (85 µg/d) compared with men (111 µg/d). Iodine intake diminished with age and increased with BMI (body mass index) in male but not in female participants, without achieving the adequate intake in any sex, age, or BMI category. In this random sample of Italian general adult population examined in 2008–2012, iodine intake still remained lower than the recommended values despite the implementation of a strategy of iodoprophylaxis based on salt iodization in 2005. These data represent a valuable reference for future monitoring of iodine status in our country.
Safety and Efficacy of Early High Parenteral Lipid Supplementation in Preterm Infants: A Systematic Review and Meta-Analysis
Authors: Kim Kyunghoon , Kim Na Jin , Kim Sae Yun
Published: May 2021

The objective of this systematic review and meta-analysis was to summarize the effects of early initiation and achievement of a high dose of parenteral lipids (≥1.5 g/kg/day reached within the first 24 h of birth) on growth and adverse outcomes in preterm infants. PubMed, EMBASE, and Cochrane databases were utilized to search for publications for this meta-analysis. Randomized controlled trials were eligible if data on growth or clinical outcome was available. The search returned nine studies. The mean proportion of postnatal weight loss (%) was lower (mean difference [MD]: −2.73; 95% confidence interval [CI]: −3.69, −1.78), and the mean head circumference near the term equivalent age (cm) was higher in the early high lipid treatment group (MD: 0.67; 95% CI: 0.25, 1.09). There was a favorable association of early high lipid administration with the incidence of extrauterine growth restriction (relative risk [RR]: 0.27; 95% CI: 0.15, 0.48). Generally, there were no differences in morbidities or adverse outcomes with early high lipid administration. Early initiation of parenteral lipids and high dose achieved within the first 24 h of life appear to be safe and endurable and offer benefits in terms of growth.
Commonalities between ENT Specialists and Oral Medicine Experts: Old HPV Diseases and New Oral HPV-Cancer along the Borders
Authors: Campisi Giuseppina , Mauceri Rodolfo , Tozzo Pietro , Panzarella Vera
Published: May 2021

A human papillomavirus (HPV) infection is globally one of the most common causes of sexually transmitted infections of the mucous membranes (genital, anal and oral). Over the last decade, an increasing number of young patients have been infected due to the changes in sexual habits in the general population. The majority of the lesions are benign; however, substantial scientific evidence has suggested a role for the HPV family in the carcinogenesis of squamous cell carcinoma (SCC). It is proposed that dentists, in addition to ENT specialists, should apply standardized management protocols in order to construct a well-defined pathway in terms of diagnosis, which is based on a PCR diagnostic technique and the management of those lesions.
The Incidence of Dirofilaria immitis in Shelter Dogs and Mosquitoes in Austria
Authors: Sonnberger Karin , Fuehrer Hans-Peter , Sonnberger Bernhard Werner , Leschnik Michael
Published: May 2021

To estimate the incidence of Dirofilaria immitis in Austrian shelter dogs and mosquitoes trapped in their proximity, 115 shelter dogs from fourteen animal shelters located in five different Austrian states were examined. Blood samples were screened for D. immitis using ELISA antigen-testing, PCR and microscopical examination for microfilariae. In total, 91% of the dogs originated from countries endemic for dirofilariosis. Eleven dogs (9.6%), all originating from Hungary, tested positive for D. immitis. None of the dogs examined showed microfilaremia. Eight dogs showed no or only mild clinical signs (e.g., infrequent coughing), and three dogs showed frequent coughing, dyspnea, exercise intolerance, blunt fur or weight loss. In total, 205 Mosquitoes of ten different species were caught at five different shelter sites in four different Austrian states, using CO2-baited mosquito traps set once a month (June–September 2019) for 24 h. All 205 mosquitoes tested negative for Dirofilaria spp. via PCR. The risk of endemisation of D. immitis in Austria (and other non-endemic countries in a similar situation) is very serious and its zoonotic potential should be communicated more strongly. To monitor a possible transmission of microfilariae from untreated or even untested positive dogs, e.g., in animal shelters, to mosquitoes in the near surroundings, frequent screening for Dirofilaria in mosquitoes should be used more intensively. Current knowledge on D. immitis should be integrated into daily veterinary practice and dog owners should be proactively educated, especially before traveling to endemic areas or adopting dogs from endemic countries. Animal shelters and animal welfare organizations should be provided with appropriate education and veterinary guidance regarding the testing and treatment of dogs imported from high-risk areas.
Evaluation of Laboratory Predictors for In-Hospital Mortality in Infective Endocarditis and Negative Blood Culture Pattern Characteristics
Authors: Buburuz Ana-Maria , Petris Antoniu , Costache Irina Iuliana , Jelihovschi Igor , Arsenescu-Georgescu Catalina , Iancu Luminita Smaranda
Published: May 2021

Objective: This study aimed to identify possible differences between blood culture-negative and blood culture-positive groups of infective endocarditis (IE), and explore the associations between biological parameters and in-hospital mortality. Methods: This was a retrospective study of patients hospitalized for IE between 2007 and 2017. Epidemiological, clinical and paraclinical characteristics, by blood culture-negative and positive groups, were collected. The best predictors of in-hospital mortality based on the receiver-operating characteristic (ROC) analysis and AUC (area under the curve) results were identified. Results: A total of 126 IE patients were included, 54% with negative blood cultures at admission. Overall, the in-hospital mortality was 28.6%, higher in the blood culture-negative than positive group (17.5% vs. 11.1%, p = 0.207). A significant increase in the Model for End-Stage Liver Disease Excluding International Normalized Ratio (MELD-XI) score was observed in the blood culture-negative group (p = 0.004), but no baseline characteristics differed between the groups. The best laboratory predictors of in-hospital death in the total study group were the neutrophil count (AUC = 0.824), white blood cell count (AUC = 0.724) and MELD-XI score (AUC = 0.700). Conclusion: Classic laboratory parameters, such as the white blood cell count and neutrophil count, were associated with in-hospital mortality in infective endocarditis. In addition, MELD-XI was a good predictor of in-hospital death.
Development of In Situ Gelling Meloxicam-Human Serum Albumin Nanoparticle Formulation for Nose-to-Brain Application
Authors: Katona Gábor , Sipos Bence , Budai-Szűcs Mária , Balogh György Tibor , Veszelka Szilvia , Gróf Ilona , Deli Mária A. , Volk Balázs , Szabó-Révész Piroska , Csóka Ildikó
Published: May 2021

The aim of this study was to develop an intranasal in situ thermo-gelling meloxicam-human serum albumin (MEL-HSA) nanoparticulate formulation applying poloxamer 407 (P407), which can be administered in liquid state into the nostril, and to increase the resistance of the formulation against mucociliary clearance by sol-gel transition on the nasal mucosa, as well as to improve drug absorption. Nanoparticle characterization showed that formulations containing 12–15% w/w P407 met the requirements of intranasal administration. The Z-average (in the range of 180–304 nm), the narrow polydispersity index (PdI, from 0.193 to 0.328), the zeta potential (between −9.4 and −7.0 mV) and the hypotonic osmolality (200–278 mOsmol/L) of MEL-HSA nanoparticles predict enhanced drug absorption through the nasal mucosa. Based on the rheological, muco-adhesion, drug release and permeability studies, the 14% w/w P407 containing formulation (MEL-HSA-P14%) was considered as the optimized formulation, which allows enhanced permeability of MEL through blood–brain barrier-specific lipid fraction. Cell line studies showed no cell damage after 1-h treatment with MEL-HSA-P14% on RPMI 2650 human endothelial cells’ moreover, enhanced permeation (four-fold) of MEL from MEL-HSA-P14% was observed in comparison to pure MEL. Overall, MEL-HSA-P14% can be promising for overcoming the challenges of nasal drug delivery.
Ion-Complex Microcrystal Formulation Provides Sustained Delivery of a Multimodal Kinase Inhibitor from the Subconjunctival Space for Protection of Retinal Ganglion Cells
Authors: Hsueh Henry T. , Kim Yoo-Chun , Pitha Ian , Shin Matthew D. , Berlinicke Cynthia A. , Chou Renee Ti , Kimball Elizabeth , Schaub Julie , Quillen Sarah , Leo Kirby T. , Han Hyounkoo , Xiao Amy , Kim Youngwook , Appell Matthew , Rai Usha , Kwon HyeYoung , Kolodziejski Patricia , Ogunnaike Laolu , Anders Nicole M. , Hemingway Avelina , Jefferys Joan L. , Date Abhijit A. , Eberhart Charles , Johnson Thomas V. , Quigley Harry A. , Zack Donald J. , Hanes Justin , Ensign Laura M.
Published: May 2021

Glaucoma is the leading cause of irreversible blindness worldwide. Elevated intraocular pressure (IOP) is one of the major risk factors for glaucoma onset and progression, and available pharmaceutical interventions are exclusively targeted at IOP lowering. However, degeneration of retinal ganglion cells (RGCs) may continue to progress despite extensive lowering of IOP. A complementary strategy to IOP reduction is the use of neuroprotective agents that interrupt the process of cell death by mechanisms independent of IOP. Here, we describe an ion complexation approach for formulating microcrystals containing ~50% loading of a protein kinase inhibitor, sunitinib, to enhance survival of RGCs with subconjunctival injection. A single subconjunctival injection of sunitinib-pamoate complex (SPC) microcrystals provided 20 weeks of sustained retina drug levels, leading to neuroprotection in a rat model of optic nerve injury. Furthermore, subconjunctival injection of SPC microcrystals also led to therapeutic effects in a rat model of corneal neovascularization. Importantly, therapeutically relevant retina drug concentrations were achieved with subconjunctival injection of SPC microcrystals in pigs. For a chronic disease such as glaucoma, a formulation that provides sustained therapeutic effects to complement IOP lowering therapies could provide improved disease management and promote patient quality of life.
Antioxidant and Cytotoxic Activities of Usnea barbata (L.) F.H. Wigg. Dry Extracts in Different Solvents
Authors: Popovici Violeta , Bucur Laura , Popescu Antoanela , Schröder Verginica , Costache Teodor , Rambu Dan , Cucolea Iulia Elena , Gîrd Cerasela Elena , Caraiane Aureliana , Gherghel Daniela , Vochita Gabriela , Badea Victoria
Published: May 2021

Lichens represent a significant source of antioxidants due to numerous metabolites that can reduce free radicals. Usnea barbata (L.) F.H. Wigg. has been recognized and used since ancient times for its therapeutic effects, some of which are based on its antioxidant properties. The present study aims to analyze the phytochemical profile and to evaluate the antioxidant and cytotoxic potential of this lichen species. Five dry extracts of U. barbata (UBDE) in different solvents (acetone, ethyl acetate, ethanol, methanol, water) were prepared by refluxing at Soxhlet to achieve these proposed objectives and to identify which solvent is the most effective for the extraction. The usnic acid content (UAC) was quantified by ultra-high performance liquid chromatography (UHPLC). The total polyphenols content (TPC) and tannins content (TC) were evaluated by spectrophotometry, and the total polysaccharides (PSC) were extracted by a gravimetric method. The 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) free radical method was used to assess the antioxidant activity (AA) and the Brine Shrimp Lethality (BSL) assay was the biotest for cytotoxic activity evaluation. The ethyl acetate extract had the highest usnic acid content, and acetone extract had the highest content of total polyphenols and tannins. The most significant antioxidant effect was reported to methanol extract, and all the extracts proved high cytotoxicity. The water extract has the lowest cytotoxicity because usnic acid is slightly soluble in this solvent, and it was not found at UHPLC analysis. All extracts recorded a moderate correlation between the content of usnic acid, polyphenols, tannins, and AA; furthermore, it has been observed that the cytotoxicity varies inversely with the antioxidant effect.
Effect of Different Climate Change Variables on the Ecology and Management of Sesbania cannabina through Glyphosate
Authors: Iqbal Nadeem , Manalil Sudheesh , Chauhan Bhagirath Singh , Adkins Steve
Published: May 2021

An elevated atmospheric carbon dioxide (CO2) concentration and frequent droughts are two anticipated climate change scenarios in which certain invasive weeds may develop competitive advantages over crops and adversely impact productivity and herbicide efficacy. Hence, a study was conducted to explore the effect of different climatic scenarios on the growth and management of Sesbania cannabina (Retz.) Pers with glyphosate. The variables investigated were two CO2 concentrations (400 and 700 ppm), two soil moisture levels (100% and 50% of field capacity (FC)), and three glyphosate rates (0 (control), 517 (50% of recommended rate), and 1034 g ae ha−1 (recommended rate)). CO2 concentrations and soil moisture levels had different effects on the growth and management of S. cannabina. Overall, 100% FC and elevated [CO2] of 700 ppm recorded the maximum plant height (38 cm), leaves per plant (20), growth index (60), chlorophyll content (SPAD value 37), and dry biomass (3 g) in comparison with ambient [CO2] of 400 ppm and 50% FC treatment. The recommended glyphosate application gave 100% weed biomass reduction; however, efficacy was reduced (63%) when applied at 50% of the recommended rate under elevated [CO2] of 700 ppm and 50% FC conditions.
Identification of Susceptibility Genes in Castanea sativa and Their Transcription Dynamics following Pathogen Infection
Authors: Pavese Vera , Moglia Andrea , Gonthier Paolo , Torello Marinoni Daniela , Cavalet-Giorsa Emile , Botta Roberto
Published: May 2021

Castanea sativa is one of the main multipurpose tree species valued for its timber and nuts. This species is susceptible to two major diseases, ink disease and chestnut blight, caused by Phytophthora spp. and Cryphonectria parasitica, respectively. The loss-of-function mutations of genes required for the onset of pathogenesis, referred to as plant susceptibility (S) genes, are one mechanism of plant resistance against pathogens. On the basis of sequence homology, functional domain identification, and phylogenetic analyses, we report for the first time on the identification of S-genes (mlo1, dmr6, dnd1, and pmr4) in the Castanea genus. The expression dynamics of S-genes were assessed in C. sativa and C. crenata plants inoculated with P. cinnamomi and C. parasitica. Our results highlighted the upregulation of pmr4 and dmr6 in response to pathogen infection. Pmr4 was strongly expressed at early infection phases of both pathogens in C. sativa, whereas in C. crenata, no significant upregulation was observed. The infection of P. cinnamomi led to a higher increase in the transcript level of dmr6 in C. sativa compared to C. crenata-infected samples. For a better understanding of plant responses, the transcript levels of defense genes gluB and chi3 were also analyzed.
Effect of Extracts from Dominant Forest Floor Species of Clear-Cuts on the Regeneration and Initial Growth of Pinus sylvestris L. with Respect to Climate Change
Authors: Sirgedaitė-Šėžienė Vaida , Marčiulynas Adas , Baliuckas Virgilijus
Published: May 2021

Climate change influences the ecological environment and affects the recruitment of plants, in addition to population dynamics, including Scots pine regeneration processes. Therefore, the impact of cover-dominant species extracts on the germination of pine seeds and morpho-physiological traits of seedling under different environmental conditions was evaluated. Increasing temperature reinforces the plant-donor allelochemical effect, reduces Scots pine seed germination, and inhibits seedling morpho-physiological parameters. Conditions unfavourable for the seed germination rate were observed in response to the effect of aqueous extracts of 2-year-old Vaccinium vitis-ideae and 1-year-old Calluna vulgaris under changing environmental conditions. The lowest radicle length and hypocotyl growth were observed in response to the effect of 1-year-old C. vulgaris and 2-year-old Rumex acetosella under increasing temperature (+4 °C) conditions. The chlorophyll a + b concentration in control seedlings strongly decreased from 0.76 to 0.66 mg g−1 (due to current environmental and changing environmental conditions). These factors may reduce the resistance of Scots pine to the effects of dominant species and affect the migration of Scots pine habitats to more favourable environmental conditions.
Sensitive, Color-Indicating and Labeling-Free Multi-Detection Cholesteric Liquid Crystal Biosensing Chips for Detecting Albumin
Authors: Wang I-Te , Lee Yen-Hua , Chuang Er-Yuan , Hsiao Yu-Cheng
Published: May 2021

A novel device for cholesteric liquid crystal (CLC)-based microfluidic chips, accommodated in a polydimethylsiloxane material, was invented. In this device, the reorientation of the CLCs was consistently influenced by the surface of the four channel walls and adjacent CLCs. When the inside of the microchannel was coated with the alignment layer, the CLCs oriented homeotropically in a focal conic state under cross-polarizers. Once antigens had bound onto antibodies immobilized onto the orientation sheet-coated channel walls, the light intensity of the CLC molecules converted from a focal conic state to a bright planar state caused by disrupting the CLCs. By means of utilizing pressure-propelling flow, the attachment of antigen/antibody to the CLCs should be detectable within consecutive sequences. The multi-microfluidic CLC-based chips were verified by measuring bovine serum albumin (BSA) and immune complexes of pairs of BSA antigen/antibody. We showed that the multiple microfluidic immunoassaying can be used for measuring BSA and pairs of antigen/antibody BSA with a detection limit of about 1 ng/mL. The linear range is 0.1 μg/mL–1 mg/mL. A limit of immune detection of pairs of BSA antigens/antibodies was 10 ng/mL of BSA plus 1000 ng/mL of the anti-BSA antibodies was observed. According to this innovative creation of immunoassaying, an unsophisticated multi-detection device with CLC-based labeling-free microfluidic chips is presented.
Poly(etheretherketone)/Poly(ethersulfone) Blends with Phenolphthalein: Miscibility, Thermomechanical Properties, Crystallization and Morphology
Authors: Korycki Adrian , Garnier Christian , Abadie Amandine , Nassiet Valerie , Sultan Charles Tarek , Chabert France
Published: May 2021

Polyetheretherketone (PEEK)/polyethersulfone (PES) blends are initially not miscible, except when the blends are prepared by solvent mixing. We propose a route to elaborate PEEK/PES blends with partial miscibility by melt mixing at 375 °C with phenolphthalein. The miscibility of blends has been examined using differential scanning calorimetry (DSC) and dynamic mechanical analysis (DMTA). When adding phenolphthalein to PEEK/PES blends, the glass transitions are shifted inward as an indication of miscibility. We suggest that phenolphthalein acts as a compatibilizer by creating cardo side groups on PEEK and PES chains by nucleophilic substitution in the melted state, although this condensation reaction was reported only in the solvent until now. In addition, phenolphthalein acts as a plasticizer for PES by decreasing its glass transition. As a consequence, the PEEK phase is softened which favors the crystallization as the increase of crystalline rate. Due to aromatic moieties in phenolphthalein, the storage modulus of blends in the glassy region is kept identical to pure PEEK. The morphological analysis by SEM pictures displays nano- to microsized PES spherical domains in the PEEK matrix with improved PEEK/PES interfacial adhesion.
A Tentative Proposal to Use Orthodox Theological Relational Selfhood as an Alternative for Confucian-Influenced Chinese Evangelicals
Authors: Hwang Tsung-I
Published: May 2021

Eastern Orthodox Christianity and Chinese evangelicals have rarely interacted. Even if it seems that Eastern Orthodox Christianity and its theology have hardly influenced Chinese evangelicals in the past, this article demonstrates the possibility that Orthodox theology can still indirectly transform Confucian-influenced Chinese evangelicals. Moltmann, a great contemporary Protestant theologian, is influenced deeply by Stăniloae, a great modern Eastern Orthodox theologian, particularly in the development of social trinitarian theology in Eastern Orthodox heritage. Moltmann argues that social trinitarian anthropology can prevent the social and individual problems appeared in the societies shaped by either individualism or collectivism. Selfhood is one academic language used to discuss this relationship between the self and society. Despite modernization and westernization, contemporary Chinese people are still deeply influenced by Confucian models of relational selfhood. Even for Chinese evangelicals who had converted years ago, their way of thinking and behavior might be as much Confucian as biblical. The Confucian-influenced collectivist mindset may lead to problematic selfhood and more challenging interpersonal relationships. This article uses Orthodox theology via Moltmann’s social trinitarian, Stăniloae-inspired approach to develop an alternative relational selfhood for contemporary Chinese Christians.
Lamination and Its Impact on the Physical and Mechanical Properties of the Permian and Triassic Terrestrial Sandstones
Authors: Figarska-Warchoł Beata , Rembiś Marek
Published: May 2021

The sandstones with a laminated structure are common building materials. Lamination is macroscopically expressed as colour and grain size variations observed both in the deposit and within individual beds; therefore, the properties of such sandstones are diverse depending on the spatial distribution of the binding mass and framework components. For the terrestrial sandstones of different genesis, four types of laminae have been distinguished based on petrographic studies. They have a siliceous binder or a mixed ferruginous–siliceous–argillaceous binder with different proportions of these components. In laminae of types I–III, the grain framework is built mainly of quartz grains, and in type IV, it is accompanied by numerous lithoclasts and feldspars. Knoop hardness and CERCHAR abrasivity were tested in each lamina variety, and the results were correlated with the equivalent quartz content and the longitudinal ultrasonic wave velocity measured perpendicular and parallel to the lamination. The proposed research methodology was not used in previous studies on terrestrial laminated sandstones. The results explain a strong dependence between mineral composition, structure of laminae, and technical parameters of rocks. The knowledge of this relationship facilitates the selection of rocks that meet the relevant technical requirements and helps to optimally manage the resources of sandstone deposits.
Acoustic Seafloor Classification Using the Weyl Transform of Multibeam Echosounder Backscatter Mosaic
Authors: Zhao Ting , Montereale Gavazzi Giacomo , Lazendić Srđan , Zhao Yuxin , Pižurica Aleksandra
Published: May 2021

The use of multibeam echosounder systems (MBES) for detailed seafloor mapping is increasing at a fast pace. Due to their design, enabling continuous high-density measurements and the coregistration of seafloor’s depth and reflectivity, MBES has become a fundamental instrument in the advancing field of acoustic seafloor classification (ASC). With these data becoming available, recent seafloor mapping research focuses on the interpretation of the hydroacoustic data and automated predictive modeling of seafloor composition. While a methodological consensus on which seafloor sediment classification algorithm and routine does not exist in the scientific community, it is expected that progress will occur through the refinement of each stage of the ASC pipeline: ranging from the data acquisition to the modeling phase. This research focuses on the stage of the feature extraction; the stage wherein the spatial variables used for the classification are, in this case, derived from the MBES backscatter data. This contribution explored the sediment classification potential of a textural feature based on the recently introduced Weyl transform of 300 kHz MBES backscatter imagery acquired over a nearshore study site in Belgian Waters. The goodness of the Weyl transform textural feature for seafloor sediment classification was assessed in terms of cluster separation of Folk’s sedimentological categories (4-class scheme). Class separation potential was quantified at multiple spatial scales by cluster silhouette coefficients. Weyl features derived from MBES backscatter data were found to exhibit superior thematic class separation compared to other well-established textural features, namely: (1) First-order Statistics, (2) Gray Level Co-occurrence Matrices (GLCM), (3) Wavelet Transform and (4) Local Binary Pattern (LBP). Finally, by employing a Random Forest (RF) categorical classifier, the value of the proposed textural feature for seafloor sediment mapping was confirmed in terms of global and by-class classification accuracies, highest for models based on the backscatter Weyl features. Further tests on different backscatter datasets and sediment classification schemes are required to further elucidate the use of the Weyl transform of MBES backscatter imagery in the context of seafloor mapping.
Editorial for the Special Issue “Remote Sensing Monitoring of Land Surface Temperature”
Authors: Sánchez Juan M. , Coll César , Niclòs Raquel
Published: May 2021

The combination of the state-of-the-art in the thermal infrared (TIR) domain [...]