bluuebunny/crossref_metadata_2025 huggingface dataset:
First Implementation of Managed Pressure Drilling (MPD) System in Swamp Barge Rig to Drill Narrow Pressure Window HPHT Exploration Well Safely – Return of Experience from Kalimantan, Indonesia
Authors: Setiohadi _ , Hadistira Praja , Yudhatama Muhammad Alfianoor , Sitompul Daniel , Hutabarat Dikha Escodianto , Wiharja Andre , Agriawan Crisa , Martadinata Aji , Sudiariaji _ , Sidianto Agus , Prasthio Andry , Irawan Fikri
Published: October 2022

The first ever HPHT exploration well in Kalimantan was drilled by Swamp Barge Rig with narrow pressure window environment. The well was planned to be drilled using 2.9 SG mud weight to 4575 m vertical depth with 0.10 SG pressure window. Maximum predicted bottom hole temperature of the well was 186° C while the maximum expected wellhead pressure (MEWHP) reached 11,300 Psi. MPD becomes mandatory to complete the well and becomes a novel implement for swamp barge rig operation. Therefore, MPD workshop was held among the teams to ensure all personnel become familiar with the system. On other hand, the equipment configuration was set with some modifications to accommodate the operation requirement, contingency, tight stack up below rotary table and hoisting devices limitation. Rotating Control Device (RCD) was stacked up part by part on tight moon pool space. Several modifications were done properly and gave operation flexibility plus robust contingency. RCD alignment was reset periodically and natural rubbers were utilized to enhanced rubbers lifetime in high temperature condition. EKD system with Coriolis utilization was being main mitigation during drilling with limited kick margin that will allow reservoir section to be accessed. Formation pressure investigation was also performed to give additional information on formation pressure for better well assessment. SBP application on dual gradient tripping and managed pressure cementing give a means to secure the well safely and properly during high gas event with losses condition. As the result, MPD implementation enables the operator to complete HPHT exploration well on swamp barge rig project safely despite the complexity and risk of narrow formation pressure window operation.
Expression of thrombin-cleaved osteopontin and integrin α9 and β1 signaling pathway molecules in chronic subdural hematomas
Authors: Osuka Koji , Ohmichi Yusuke , Ohmichi Mika , Suzuki Chiharu , Aoyama Masahiro , Iwami Kenichiro , Watanabe Yasuo , Honma Satoru , Miyachi Shigeru
Published: October 2022

Chronic subdural hematoma (CSDH) is considered to be an inflammatory and angiogenic disease. The CSDH outer membrane, which contains inflammatory cells, plays an important role in CSDH development. Osteopontin (OPN) is an extracellular matrix protein that is cleaved by thrombin, generating the N-terminal half of OPN, which is prominently involved in integrin signal transduction. We explored the expression of the N-terminal half of OPN in CSDH fluid and the expression of integrins α9 and β1 and the downstream components of the angiogenic signaling pathways in the outer membrane of CSDHs. Twenty samples of CSDH fluid and 8 samples of CSDH outer membrane were included. The concentrations of the N-terminal half of OPN in CSDH fluid samples were measured using ELISA kits. The expression levels of integrins α9 and β1, vinculin, talin-1, focal adhesion kinase (FAK), paxillin, α-actin, Src and β-actin were examined by western blot analysis. The expression levels of integrins α9 and β1, FAK and paxillin were also examined by immunohistochemistry. We investigated whether CSDH fluid could activate FAK in cultured endothelial cells in vitro. The concentration of the N-terminal half of OPN in CSDH fluid was significantly higher than that in the serum. Western blot analysis confirmed the presence of these molecules. In addition, integrins α9 and β1, FAK and paxillin were localized in the endothelial cells of vessels within the CSDH outer membrane. FAK was significantly phosphorylated immediately after treatment with CSDH fluid. Our data suggest that the N-terminal half of OPN in CSDH fluid promotes neovascularization in endothelial cells through integrins α9 and β1. The N-terminal half of OPN, which is part of the extracellular matrix, plays a critical role in the promotion of CSDH.
A FCM-based image de-noising with spatial statistics pilot study
Authors: Chen Tzong-Jer
Published: October 2022

Image de-noising is an important scheme to make the image visually prominent and obtain enough useful information. To obtain reliable results, many applications had developed for effective noise suppression and received good image quality. This report assumed a residual image consisted of noises with edges produced by subtracting the original and a low-pass filter smoothed image. The Moran statistics was then using to measure the variation of spatial information in residual images and then as a feature data input to FCM. Three clusters pre-assumed for FCM in this work: they are heavy, medium and less noisy areas. The rates of each position partially belongs to each cluster were determined by a FCM membership function. Each pixel in noisy image assumed in de-noising processing that which is a linear combination of product of three de-noised images with membership function in the same position. Average filters with different windows and a Gaussian filter priori applied to this noisy image to make three de-noised versions. The results showed that this scheme worked better than the non-adaptive smoothing. This scheme‘s performance is evaluated and compared to the Bilateral filter and NLM using PSNR and SSIM. The developed scheme is a pilot study on this area. Further future studies needed on the optimized number of clusters and smoother versions used in linear combination.
Evaluation of the disinfection effect and mechanism of SO4•- and HO• in UV / persulfate salts from the perspective of VBNC bacteria
Authors: Huang Zaihui , Qi Zheng , Liu Chunguang
Published: October 2022

Although ultraviolet (UV) and persulfate (PS) have been widely used in water disinfection process, their incompleteness of disinfection, such as inducing the production of viable but non-culturable cells (VBNC), has attracted extensive attention. In this study, the disinfection effect of combined UV and PS was evaluated from the perspective of inducing Pseudomonas aeruginosa into VBNC, and the roles of SO4•− and HO• radicals in UV/PS disinfection were also analyzed. UV/PS more effectively inactivated cells and reduced the number of culturable cells, but induced more VBNC cells. On the other hand, the test of bacterial dark activation suggested that UV/PS disinfection effectively prolongs the recovery time of VBNC cells. The mechanisms of UV/PS disinfection were the increase of membrane permeability and oxidative stress, where SO4•− radicals played more role than HO• radicals. Furthermore, UV/PS disinfection more significantly perturbed the metabolism of Pseudomonas aeruginosa (p < 0.05), mainly involving glyoxylate and dicarboxylic acid metabolism, aminoacyl-tRNA biosynthesis, Alanine, aspartate and glutamate metabolism, citric acid cycle (TCA cycle). This study, from the production and recovery of VBNC, provides a new idea for the evaluation of UV, persulfate and their combined disinfection effect, and provides guidance for improving disinfection process.
Reducing Energy Consumption in Bluetooth 5 Protocol for IoT Applications using an Adaptive Model
Authors: Ferizhandi Amin Mortezaie , Oskuie Alireza Sinaiie , Zarepour Eisa , Mozayani Naser
Published: October 2022

Nowadays, the use of IoT-based approaches is expanding to the point where they have a variety of applications with various proposals such as monitoring and controlling things. Furthermore, many of these solutions were applicable to the application; there are other challenges, the most significant of which are energy consumption and covered area. Various investigations have been conducted to date in evaluating capabilities and improving the functionality of BLE from delay and throughput to reliability. Nonetheless, they have not considered managing energy consumption with interference in BLE 5. As a result, this paper proposes an adaptive model for managing energy consumption in BLE 5. We measured energy consumption in connection events using various types of output power and PHY in an experiment. Finally, by using the adaptive model in the mobility scenario in the IUST football stadium, we reduced 13.4% of energy consumption and 1% of missed events rather than criteria mode, which had an average of 5% missed events. In the other scenario, we reduced energy consumption by 18.7% while increasing missed events by 1% rather than criteria mode and an average of 3% in missed events.
Effects of Family-based Dignity Intervention and Expressive Writing on Anticipatory Grief in Family Caregivers of Patients with Cancer: A Randomized Controlled Trial
Authors: Najafi Tahereh , seyedfatemi Naeima , Kamyari Naser , Bolhari Jafar , rezaei masoud
Published: October 2022

Family caregivers of dying cancer patients may suffer from grief experiences and bereavement complications. Previous studies have proposed some psycho-emotional interventions for the management of these complications. However, little attention has been given to family-based dignity intervention and expressive writing. This study was conducted to examine the effects of family-based dignity intervention and expressive writing, combined and alone, on anticipatory grief in family caregivers of dying cancer patients. This was a randomized controlled trial, in which 200 family caregivers of dying cancer patients were randomly assigned to the 4 intervention groups: family-based dignity intervention (n = 50), expressive writing intervention (n = 50), combined family-based single dignity intervention and expressive writing (n = 50), and control group (n = 50). At baseline, 1 week and 2 weeks after the interventions, anticipatory grief was assessed by a 13-item anticipatory grief scale (AGS). We found a significant reducing effect of family-based dignity intervention on AGS (-8.12 ± 1.53 vs. -1.57 ± 1.52, P = 0.01) and its subscales including behavioral (-5.92 ± 0.97 vs. -2.17 ± 0.96, P = 0.04) and emotional (-2.38 ± 0.78 vs. 0.68 ± 0.77, P = 0.03) subscales compared with the control group. However, no significant effect was seen for expressive writing intervention and combined interventions of expressive writing and family-based dignity therapy. In conclusion, family-based dignity therapy may be a safe and low-cost intervention for relieving anticipatory grief among family caregivers of dying cancer patients. Additional clinical trials are needed to confirm our findings. Registration number: IRCT20210111050010N1.
A fast ceramic mixed OH-/H+ ionic conductor for low temperature fuel cells
Authors: Tao Shanwen , Zou Peimiao , Iuga Dinu , Chen Shigang , Zhang Mengfei , Han Yisong
Published: October 2022

OH- and H+ ionic conductors are important electrolyte materials for electrochemical devices such as fuel cells. The high cost of the best low temperature H+ ionic conductor, Nafion membrane, and the poor chemical compatibility with CO2 in air of alkaline membrane based on quaternary ammonium groups have seriously affected the large-scale application of low temperature fuel cells. Here we show the discovery of a fast ceramic mixed OH-/H+ conductor, perovskite oxide SrZr0.8Y0.2O3-δ, which exhibits a high ionic conductivity of approximately 0.01 S cm-1 at 90°C when measured in water and wet air, sufficient to be used as electrolyte for low temperature fuel cells. The ionic conductivity is stable in wet air during the measured 130 hours. The ionic conduction was also demonstrated by near ambient temperature solid oxide fuel cells (NAT-SOFCs). This opens a window on discovering new ionic conducting materials for low temperature fuel cells.
Nitrogen Atmospheric Deposition Driven by Seasonal Processes in a Brazilian Region With Agricultural Background
Authors: Pereira Jaqueline Natiele , Mantovani Vanessa Alves , de Mello Carlos Rogério , Fornaro Adalgiza , Vieira-Filho Marcelo
Published: October 2022

Understanding the seasonal patterns and influencing factors of nitrogen atmospheric deposition is essential to evaluate human impacts on the air quality and nitrogen biogeochemical cycle. However, evaluation of the nitrogen deposition flux, especially in South America agricultural regions, has not been fully investigated. In this paper, we quantified the atmospheric wet deposition fluxes of total dissolved nitrogen (TDN), dissolved organic nitrogen (DON) and dissolved inorganic nitrogen (DIN), in a region with agricultural and livestock predominance in the Southern Minas Gerais region, Brazil, from May 2018 to April 2019. Deposition fluxes of nitrogen species in the wet season (October – March) were on average 4.8 fold higher than dry season, which revealed significant seasonal variations driven largely by the seasonality of rainfall and agricultural operations. We also found high NO3−/NH4+ ratios (average = 8.25), with higher values in dry season (NO3−/NH4+ = 12.8) in comparison with wet season (NO3−/NH4+ = 4.48), which revealed a higher relative contribution of NOx emissions from traffic sources in dry season. We also estimated the influence of atmospheric deposition of inorganic nitrogen (N-DIN) on environmental ecosystems, being 2.01 kgNha− 1yr− 1 with potential risk of acidification and eutrophication of 30%. Therefore, attention should be paid to the role of wet atmospheric deposition of nitrogen as a source of nitrogen environmental pollution in agricultural regions.
Cross-shore changes in upwelling from
offshore wind farm development in California
Authors: Raghukumar Kaustubha , Nelson Timothy , Jacox Michael , Chartrand Christopher , Fiechter Jerome , Chang Grace , Cheung Lawrence , Roberts Jesse
Published: October 2022

In California offshore waters, sustained northwesterly winds have been identified as a key energy resource that could contribute substantially to California’s renewable energy goals. However, the development of large-scale offshore wind farms has the potential to reduce the wind stress at the sea surface, which could have implications on wind-driven upwelling, nutrient delivery, and ecosystem dynamics. Using atmospheric and ocean circulation models together with a hypothetical upper boundary buildout scenario of 877 turbines spread across three wind energy areas of interest, wind speed reductions in the lee of the wind energy areas of interest are found to enhance horizontal wind stress shear, leading to reduced upwelling on the inshore side of windfarms and increased upwelling on the offshore side. These changes, when expressed in terms of widely used metrics for upwelling volume transport and nutrient delivery, show that while the net upwelling in a wide coastal band changes relatively little, the spatial structure of upwelling within this coastal region can be shifted outside the bounds of natural variability.
Efficacy and safety of TNF inhibitors in the treatment of juvenile idiopathic arthritis: a systematic literature review
Authors: Horneff Gerd , Minden Kirsten , Rolland Catherine , Daly Ana C. Hernandez , Borlenghi Cecilia , Ruperto Nicolino
Published: October 2022

Objective: A systematic literature review was conducted to summarize efficacy and safety data from studies that evaluated tumor necrosis factor inhibitors in patients with juvenile idiopathic arthritis (JIA).
Methods: Relevant publications were identified via online searches (cutoff: March 16, 2021). After screening search results, outcome data were extracted if the treatment arm included ≥30 patients. Outcomes were described narratively, with efficacy assessed by JIA-American College of Rheumatology (ACR) response criteria and safety assessed by the incidence of serious adverse events (SAEs) per 100 patient-years (100PY).
Results: Among 87 relevant publications included in the qualitative synthesis, 19 publications described 13 clinical trials. Across the 13 trials, the percentages of patients who achieved JIA-ACR30/50/70/90 responses at Week 12 with adalimumab ranged 71–94%, 68–90%, 55–61%, and 39–42%, respectively; with etanercept (Week 12), 73–94%, 53–78%, 36–59%, and 28%; with golimumab (Week 16), 89%, 79%, 66%, and 36%; and with infliximab (Week 14), 64%, 50%, and 22% (JIA-ACR90 not reported). SAE incidence across all time points ranged 0–13.7 SAE/100PY for adalimumab, 0–20.0 SAE/100PY for etanercept, and 10.4–24.3 SAE/100PY for golimumab (1 study). SAE incidence could not be estimated from the 2 infliximab publications.
Conclusion: Tumor necrosis factor inhibitors are effective and well tolerated in the treatment of JIA, but additional evidence from head-to-head studies and over longer periods of time, especially in the context of the transition from pediatric to adult care, would be useful.
Effect of extraction pre-treatment of larch bark on the structural and electrochemical characteristics of the carbon product
Authors: Tsyganova Svetlana , Mazurova Elena , Fetisova O'lga
Published: October 2022

This paper presents the synthesis of carbon material from the untreated and extracted larch bark. The influence of the extracting reagents (water, ethanol, hydrogen peroxide, and sodium hydroxide) on the structural changes of ash components of the bark was revealed. It is assumed that one of the reasons for the development of the porous structure of carbon products during carbonization of the extracted bark is associated with a decrease in ash content. It was found that the carbon product obtained from washed-out water bark has the highest value of the specific surface area (401 m2/g) and the largest accumulation of electric charge.
Initial ticagrelor monotherapy strategy is effective and safe in patient with high bleeding risk after percutaneous coronary intervention: series case reports and literature review
Authors: Cui Jianguo , Wu Xu , Jin Qinhua , Yang Xia , Qian Geng , Tian Feng , Sun Zhijun
Published: October 2022

Objective: Short-term duration of dual antiplatelet therapy (DAPT) followed by ticagrelor monotherapy after percutaneous coronary intervention (PCI) among patients at high bleeding risk (HBR) was identified in numerous clinical trials, but the effect of ticagrelor monotherapy as an initial regimen was unknown. To explore the efficacy and safety of ticagrelor monotherapy after PCI in patient at HBR.
Patients and methods: This were case series report at single center, unblinded without control group. A total of 12 acute coronary syndrome (ACS) patients at HBR between November 2020 and December 2021 were enrolled,17 newer generation drug-eluting stents (DES),2 drug-coated balloon (DCB) and 1 bioresorbable scaffolds (BRS) were implanted. Ticagrelor monotherapy was prescribed as an initial antiplatelet strategy after PCI. Study endpoints were major adverse cardiovascular events (MACEs) and major bleeding events at 1-month to 1-year follow-up.
Results: During hospitalization and at the 1–3-month follow-up, there were no adverse clinical events, including ischemic or major bleeding events, in any of the patients. Six patients completed 1-year clinical event-free follow-up, 2 of them had well angiographic outcomes after PCI for one year.
Conclusions: Ticagrelor monotherapy as an initial antiplatelet therapy was safety and efficacy in these patients at HBR undergoing PCI. Further prospective studies and randomized clinical trials are warranted.
Resection of Bladder Tumors at the Ureteral Orifice Using a Hook Plasma Electrode: A Case Report
Authors: Wang Ziyong , Bi Ying , Wang Qilin , Bai Yu , Li RuiQian , Yang Hong , Li Jun
Published: October 2022

Background
The traditional surgical approach for non-muscle-invasive bladder cancer is transurethral resection of bladder tumors using annular plasma electrodes, but we have innovatively used a hook electrode and proposed a new surgical method.
Case presentation
We assessed a 31-year-old male patient diagnosed with bladder cancer whose pathological type was low-grade urothelial carcinoma. MRI examination suggested that the patient's bladder tumor was located in the right ureteral orifice, and the tumor stage was T1. Subsequently, we used a homemade hook electrode to enucleate the tumor en bloc on the basis of accurately finding a gap between the bottom of the tumor and normal tissue. Meanwhile, we exposed the complete intramural ureter from the bladder wall and finally removed it.
Conclusions
Through this case of a patient, we explored a resection method similar to "sculpting" and achieved good surgical results. The hook electrode is a surgical tool worth promoting, and using it helps us to remove tumors precisely from the bladder wall.
Association between Type of Drinking Water and Upper Gastrointestinal Cancer Incidence in the Linxian General Population
Authors: Yang Huan , Wang Jian-bing , Wang Xiao-kun , Fan Jin-hu , Qiao You-Lin
Published: October 2022

Background

We aims to explore the association between type of drinking water and risk of upper gastrointestinal (UGI) cancer, including esophageal cancer (EC) and gastric cancer (GC) incidence in the Linxian General Population Nutrition Intervention Trial (NIT) cohort.

Methods

This study included healthy adults aged 40 to 69 years from the NIT cohort. All subjects were enrolled in April 1986 and followed through to March 2016. Subjects who drank tap water was treated as an exposed group whereas the remainders were considered as a comparison group. Hazard ratios (HRs) and 95% confidence intervals (95% CIs) were estimated using the Cox proportional hazard model.

Results

During the 30-year follow-up, we identified 5463 UGI cancer cases. After adjusting for age at baseline, gender, body mass index, smoking, and alcohol drinking, family history of UGI cancer, education level, nutrition intervention arms, and intakes of fruit and vegetable, the incidence rate of UGI cancer in participants who drank tap water was significantly lower than that in participants who did not drink tap water (HR = 0.88, 95%CI: 0.83–0.94). Comparable association was observed between tap water drinking and EC incidence (HR = 0.86, 95%CI: 0.79–0.94). For EC incidence, an interaction effect was observed for riboflavin/niacin supplements and type of drinking water (Pinteraction=0.03). No association was observed between type of drinking water and GC incidence.

Conclusions

Drinking tap water was associated with decreased risk of UGI cancer incidence particularly the of EC incidence. Appropriate measures should be taken to improve the quality of drinking water in high-incidence areas of EC.

Trial registration

The trial is registered with ClinicalTrials.gov (NCT00342654, 21/06/2006), and the trial name is Nutrition Intervention Trials in Linxian Follow-up Study.
The Disturbance Factor of Hoek-Brown Failure Criterion in Dam Foundations
Authors: Demirdogen Sarper , Yildirim Sefa
Published: October 2022

Dam engineers frequently use the Hoek-Brown failure criterion to estimate foundation parameters of highly jointed rock masses. The disturbance factor of Hoek-Brown criterion is considered to evaluate the effects of blasting and stress relief on rock masses. Defining this strength reduction parameter has been a long-running issue in current dam engineering practice. Some preliminary guidelines for determining disturbance factors for open-pit mining, tunnels, and slope applications are available in the literature. However, the disturbance factor has not been extensively evaluated in dam foundations where conventional mechanical excavation or controlled blasting techniques are used. In this paper, it is (1) investigated what the effects of disturbance might be on dam foundations and how they differ from other practices, (2) developed a comprehensive dataset to relate rock mass properties and disturbance types to disturbance factors assigned in dams, and (3) evaluated the influence of disturbance factor on dam design by performing stability analysis. Considering all findings with field observations of disturbance effects on dam foundations, a preliminary guideline is provided for dam engineers to determine an appropriate range of disturbance factors for their applications.
SRMDS: Secure and Reliable Medical Data Sharing Using Hybrid Cryptography with Block Chain in E-Health Systems
Authors: Madhavi M. , Sasirooba T. , Kumar G. Kranthi
Published: October 2022

The authors have requested that this preprint be removed from Research Square.
VE-Cadherin modulates β-catenin/TCF-4 to enhance Vasculogenic Mimicry
Authors: Bellido Daniel Delgado , Zamudio-Martínez Esteban , Fernandez-Cortes Monica , Herrera-Campos Ana Belen , Olmedo-Pelayo Joaquin , Jordan-Perez Carmen , Exposito Jose , de Álava Enrique , Amaral Ana Teresa , O´Valle Francisco , García-Díaz Angel , Oliver F. Javier
Published: October 2022

Vasculogenic Mimicry (VM) refers to the capacity to form blood network from aggressive cancers cells in an independent way of endothelial cells, to provide nutrients and oxygen leading to enhanced microenvironment complexity and treatment failure. In a previous study we demonstrated that VE-Cadherin and its phosphorylation at Y658 of VE-Cadherin modulated kaiso-dependent gene expression (CCND1 and Wnt 11) by regulation of Focal Adhesion kinase (FAK). In the present study, using a proteomic approach, we have found that β-catenin/TCF-4 is associated with nuclear VE-cadherin and enhances the capacity of malignant melanoma cells to undergo VM in cooperation with VE-Cadherin; in addition, preventing the phosphorylation of Y658 of VE-cadherin upon FAK resulted in VE-Cadherin/β-catenin complex dissociation, increased β-catenin degradation while reducing TCF-4-dependent genes transcription (C-Myc and Twist-1). Uveal melanoma cells knockout for VE-Cadherin loses β-catenin expression while the rescue of VE-Cadherin (but not of the phosphorylation defective VE-Cadherin Y658F mutant) permits stabilization of β-catenin and tumor growth reduction in vivo experiments. In vivo, the combined treatment with the FAK inhibitor PF271 and the anti-angiogenic agent bevacizumab leads to a strong reduction in tumor growth respect to the single treatment. In conclusion, the aberrant expression of VE-Cadherin in metastatic melanoma cells (from both uveal and cutaneous origins), together with its permanent phosphorylation of Y658 by FAK, favors the induction of the aggressive VM phenotype through its interaction with β-catenin to VE-Cadherin and by enhancing TCF-4 genes-dependent transcription
Social determinants impeding rural Indian women from participating in longitudinal health research: An observational study
Authors: Arun Aarthi , Prabhu Manohar Prasad
Published: October 2022

The social determinants of health have become an increasingly crucial public health topic in recent years and refer to the non-medical factors that affect an individual’s health outcomes. Our study focuses on understanding the various social determinants of health that most affect maternal well-being. We surveyed rural Indian women to understand their reasons for not participating in a public health research study aimed to improve their maternal outcomes. We found that the most frequent reasons cited by the women were: lack of spousal support (53.2%), lack of family support (27.9%), not having enough time (17.0%), and having a migratory lifestyle (14.8%). We also found an association between the following determinants: women who had lower education levels, were primigravida, younger, or lived in joint families were more likely to cite a lack of a husband or family support. We determined through these results that a lack of social support, time, and stable housing were the most pressing determinants of health preventing the women from maximizing their health outcomes. Future research should focus on possible programs to equalize the negative effects of these social determinants to improve the healthcare access of rural women.
A facile supercritical fluid synthesis of cobalt sulfide integrated with MXene and PANI/PDOT nanocomposites as electrode material for supercapacitor applications
Authors: sabbanahalli chetana , Upadhyay Sanjay , Joshi Naveen Chandra , Kumar Niraj , Choudhary Priyvart , Sharma Nishesh , Thaku Vikas N.
Published: October 2022

We report the synthesis of ternary CoS/MXene/PANI and CoS/MXene/ PEDOT composites using supercritical fluid (SCF) method for the first time. These fabricated materials along with CoS/MXene was gone through electrochemical investigations using three electrode system. Enhancement in specific capacitance was observed with inclusion of PANI (407 F/g) and further enhanced with PEDOT (630 F/g) at 2 A/g. Therefore, symmetric device using coin cell technique was fabricated by taking CoS/MXene/PEDOT and CoS/MXene/PANI as electrode material. These coin cells were further be examined on the basis of CV, GCD and EIS and it was observed that the specific capacitance was enhance in CoS/MXene/PEDOT (331.1 F/g) over CoS/MXene/PANI (246 F/g) at 2 A/g. For the material, the capacitance retention was calculated to evaluate the charge storage stability and it was observed that the CoS/MXene/PEDOT (97%) is slightly higher stable than CoS/MXene/PANI (96%).
Newly diagnosed multiple myeloma patients with CD56 expression benefit more from autologous stem cell transplantation
Authors: Geng Chuanying , Zhou Huixing , Wang Huijuan , Li Yanchen , Leng Yun , Zhang Zhiyao , Jian Yuan , Yang Guangzhong , Chen Wenming
Published: October 2022

Background
Several studies showed that lack of CD56 expression was a poor prognostic factor for patients with newly diagnosed multiple myeloma (NDMM). However, other studies were not able to confirm the prognostic value of CD56 in NDMM. This study aimed to evaluate the prognostic value of CD56 expression for patients with NDMM who received autologous stem cell transplantation (ASCT).
Methods
We retrospectively analyzed 394 patients with NDMM under 66 years old and the propensity score matching technique was used to reduce the bias between two groups.
Results
CD56 expression was observed in 265 (67.3%) patients, and 175 (44.4%) patients received ASCT. 44.9% (119/265) CD56 positive patients received ASCT; and 43.4% (56/129) CD56 negative patients received ASCT. Univariate and multivariate analyses showed that ASCT was correlated with longer OS (p < 0.001) and PFS (p < 0.001) for CD56 positive patients. ASCT may improve PFS of CD56 negative patients in univariate analysis, but it had no impact on PFS in multivariate analysis. Moreover, ASCT could not improve OS of CD56 negative patients in univariate and multivariate analysis (p > 0.05). In the propensity score matching analysis, 216 patients with CD56 expression were identified, 108 patients had received ASCT and 108 patients had no ASCT. Among 129 patients without CD56 expression, 80 patients, 40 in each group, were identified. Among 216 matched patients with CD56 expression, patients with ASCT had longer OS (87.6 vs.56.1 months, p < 0.001) and PFS (40.4 vs.27.6 months, p = 0.003). However, ASCT had no impact on OS and PFS for matched patients without CD56 expression (p > 0.05).
Conclusions
These results demonstrated that ASCT may improve OS and PFS of patients with CD56 expression and had little impact on survival of CD56 negative patients.
Converting animated image colors into product colors: An eye- tracking technology and MLP-based color matching design method
Authors: Hua Yinhong , Ni Jianlin , Lu Honglei
Published: October 2022

Images are a significant source of inspiration for designers to carry out the color design. However, the absence of animated images in the product color design can create confusion for designers. To convert animated image colors to product colors, this work a hybrid method of using the eye-tracking technology to assist color extraction and an intelligent algorithm to screen out the best color scheme. Firstly, the eye-tracking technology is adopted to collect the hot spot distribution of subjects while watching the animated images. According to the eye movement hot spot distribution, the most interesting animated color is extracted. And then, with the help of multilayer perceptron neural network, the color design of three-color children’s shopping cart product is carried out on the extracted animated color. Experimental results show that the color extraction based on the eye-tracking technology and the color scheme screening based on the intelligent algorithm can realize the effective conversion from animated image colors to product colors. This work proposes a color scheme design method from animations to products, which further expands the image color sources in product color design and can quickly and accurately find the color scheme that matches the animated image and the product.
Axial Involvement in Enthesitis-Related Arthritis: Results from a Single-Center Cohort
Authors: Guo Yanli , Fang Yuying , Zhang Tonghao , Pan Yuting , Wang Panpan , Fan Zhidan , Yu Haiguo
Published: October 2022

Background: Axial involvement in children with enthesitis-related arthritis (ERA) has characteristics that differ from those of peripheral involvement. This study characterized their clinical characteristics and treatment.
Methods: Patients with ERA at the Children’s Hospital of Nanjing Medical University between January 2018 and December 2020 were included. The ERA cohort was divided into two based on the presence or absence of axial joint involvement. Demographic characteristics, clinical features, and treatments were described and compared.
Results: In total, 105 children with ERA were enrolled (axial ERA, n=57; peripheral ERA, n=48). The age at disease onset of the axial group tended to be higher (11.93 ± 1.72 vs. 11.09 ± 1.91 years) and the diagnosis delay was bigger in patients with axial ERA (10.26 ± 11.66 months vs. 5.13±7.92 months). The inflammatory marker levels were significantly higher in patients with axial. There were no differences in HLA-B27 positivity between the groups (34 [59.65%] vs. 28 [58.33%], P>0.05). Hip involvement was more frequent in the axial group (52.63% vs 27.08%; X2=7.033). A total of 38 (66.67%) and 10 (20.83%) patients with axial and peripheral ERA, respectively, were treated with biological disease-modifying anti-rheumatic drugs (DMARDs) at diagnosis. The administration of biologics increased gradually in the axial ERA group, peaking at 18 months and decreasing thereafter, whereas that in the peripheral ERA group peaked at 6 months and began to decline thereafter.
Conclusions: Axial ERA is a persistent active disease and requires a more aggressive treatment. Classification and early recognition of axial involvement may help with timely diagnosis and appropriate management.
Spatial identification and multilevel zoning of land use functions improve sustainable regional management: A case study of the Yangtze River Economic Belt, China
Authors: Gao Yunxiao , Wang Zhanqi , Zhang Liguo , Chai Ji
Published: October 2022

The quantitative identification and zoning management of land use functions (LUFs) are important starting points for solving the problems of resource allocation and sustainable development. In this study, with the Yangtze River Economic Belt (YREB) as a case study area, LUFs were grouped into three primary categories: economic function (ENF), social function (SCF), and ecological function (ELF). The least square error model was adopted to identify the morphological changes of LUFs. A two-dimensional discriminant matrix of the dynamic degree of LUF change and terrain niche index was constructed to explain the terrain gradient effect of LUFs. Bivariate local spatial autocorrelation was used to analyze the trade-offs in 2018 between ELF and ENF, and ELF and SCF. Finally, a new multilevel zoning scheme for LUFs was proposed. The results showed that from 1990 to 2018, ENF increased rapidly in cities along the Yangtze River, the overall level of SCF declined, and ELF in the south of the Yangtze River was better than that in the north. LUFs’ morphological zoning exhibited significant regional differences. SCF-ELF combination areas and ELF dominance areas were mainly optimized in the second-level zoning. The areas with weak ELF were concentrated in the east of the YREB. Based on these results, nine kinds of LUF zonings and six kinds of major functional zonings were devised, and policy allocation was arranged for each zoning to improve the efficiency of spatial zoning management. Our research provides a reference for large-scale regional sustainable development and land use zoning management.
Chemoproteomic Mapping of Glycolytic Targetome in Cancer Cells
Authors: Hao Haiping , Tian Yang , Wan Ning , Zhang Hanqing , Shao Chang , Ding Ming , Bao Qiuyu , Hu Haiyang , Sun Huiyong , Zhou Kun , Chen Shuai , Wang Guangji , Ye Hui
Published: October 2022

Hyperactivated glycolysis is a metabolic hallmark of most cancer cells. Although sporadic information has revealed that glycolytic metabolites possess non-metabolic functions as signaling molecules, it remains largely elusive how these metabolites interact with and functionally regulate their binding targets. Here we introduce a Target Responsive Accessibility Profiling (TRAP) approach that measures ligand binding-induced accessibility changes for target identification through globally labeling reactive proteinaceous lysines. With TRAP, we mapped 913 target candidates and 2,487 interactions for 10 major glycolytic metabolites in a model cancer cell line. The wide targetome depicted by TRAP unveils diverse regulatory modalities of glycolytic metabolites involving direct perturbation of carbohydrate metabolism enzymes, intervention of orphan transcriptional proteins’ interactions, and modulation of targetome-level acetylation. These results deepen our understanding of how glycolysis orchestrates signaling pathways in cancer cells in support of their survival and inspire the exploitation of the glycolytic targetome for cancer therapy development.
Blood-brain barrier permeability and increased levels of amino acids in cerebrospinal fluid are associated with brain alterations in rats with acute liver failure
Authors: Guazzelli Pedro Arend , Fachim Felipe dos Santos , Travassos Anderson Santos , Schaukoski Caroline Casagrande , Ferreira Pâmela Cristina Lukasewicz , Fontella Fernanda Uruth , de Assis Adriano M , Souza Diogo Onofre
Published: October 2022

Acute liver failure (ALF) is a life-threatening medical condition that often leads to hepatic encephalopathy (HE). Animals and humans with HE have shown elevated cerebrospinal fluid (CSF) levels of glutamine and glutamate, which may be associated with brain impairment. In this study, we aim to evaluate the relationship between blood-brain barrier (BBB) integrity and CSF amino acid levels with the neurological status of rats after subtotal hepatectomy. Adult male Wistar rats underwent a subtotal hepatectomy (removing 92% of hepatic mass or SHAM group) and were divided into 4 (four) cohorts. Animals with ALF presented severe neurological impairment and high mortality rates when compared to the SHAM group (Cohort 1). We performed a hepatic function test 24 hours after subtotal hepatectomy, which demonstrated a significant increase of Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Total Bilirubin, Direct Bilirubin, Prothrombin time and Ammonia levels in blood (Cohort 2). Additionally, the increase of amino acids, glutamine, and albumin levels in CSF as well as of Evans Blue penetration into the brain tissue was correlated with the neurological grades of HE, indicating signs of impaired BBB permeability induced by ALF (Cohort 3). Furthermore, the animals’ mortality rate showed a positive correlation with the increase of amino acid levels in CSF following subtotal hepatectomy (after 12h, Cohort 4). Our data highlight the potential role of amino acid levels in CSF, especially glutamine, for detection purposes even in the early stages of HE and suggest that these molecules offer prognostic value for patients progressing to coma and death.
Prediction of bolt missing fault for multistage rotor by experimental test and analysis
Authors: YUE Cong , CHI Huashan , FAN Jianyu , ZHENG Xiangmin , ZHANG Ziling
Published: October 2022

The high-pressure rotor of aero-engine is assembled by numerous bolts under high manufacture precision. The connected structure is subjected to both axial force and transverse vibration during service, which may result in individual bolt loosen. In this study, the influence of bolt missing on the dynamic characteristics is analyzed by numerical simulation. A test rig capable of impact and frequency sweeping experiment under axial tension was constructed. The vibration response features in the simulation were then extracted. The loss function of the mean absolute error and the decision method of extreme gradient boosting were used to predict the bolt missing position. The results show that the proposed model can reach a prediction precision of more than 90%. Moreover, the coefficient of determination evaluation index of the prediction effect reaches 0.9, which is significantly higher than those of other conventional models such as multivariate linear regression and multivariate adaptive regression spliness.
Levels, sources, and risk assessment of PAHs residues in soil and plants in urban parks of Northwest China
Authors: Zhong Naifu , Zhou Xiaolong , Ailijiang Nuerla , Mamat Anwar , Chang Jiali , Cao Shuangyu , Hua Zhenyu , Li Nanxin
Published: October 2022

Using eight typical parks in Urumqi in Northwest China as the study area, we detected the content of polycyclic aromatic hydrocarbons (PAHs) in park topsoil and 10 common plants in the park during different seasons. The results showed that the content of PAHs in park soil in the summer was 5–6 times that in the winter, and the monomer PAHs in some park soil sampling points were higher than the soil pollution risk screening value. Compared with other areas, pollution is more serious. In summer, the plants with high PAHs content in leaves are short herbs, while in winter, they are tall arbors. The composition of park soil PAHs is mainly from the source of the HMW as the source of traffic. The proportion of the LMW in the winter was significantly higher than that in the summer. The source of plants in the summer is similar to that of the soil; whereas the source of plants in the winter is more complicated. The TEQ values of soil PAHs in South Park, Zhiwu Park, Shihua Park and Toutunhe Park were higher. The NIPI values of some sampling points in these four parks in the summer were relatively high. The average ILCRs of adults and children in all parks reached a low-risk level in summer. The carcinogenic risk in children is much higher than that of adults.
One step thoracotomy approach for atrial-esophageal fistula repair without cardiopulmonary bypass
Authors: Lee June , Yoon Jeashin , Hong Seok Beom , Kim Yong Han , Kim Hwan Wook , Kim Do Yeon
Published: October 2022

Although atrial-esophageal fistula is an extremely rare disease, it is a life-threatening complication after catheter ablation for atrial fibrillation. There is no consensus on management or repair
for atrial-esophageal fistula which has a high mortality rate. Here, we describe a lateral thoracotomy approach focused on simplifying the repair procedure for atrial-esophageal fistula with two cases.
Two male seniors in their 60s and 70s, respectively, who had undergone catheter ablation for atrial fibrillation presented to the emergency department with fever and mental decrease. Blood culture confirmed Streptococcal bacteremia. Brain magnetic resonance imaging showed multiple cerebral infarcts. After demonstrating atrial-esophageal fistula with computed tomography of chest, an emergency operation was planned.
The two patients were placed in the right down decubitus position with single lung ventilation. A lateral thoracotomy was performed on the left 5th intercostal space. Multiple pledgeted 4-0 prolene sutures for ligation were paced on the fistula of the pericardial side. From the esophageal side, a fistulectomy was performed, followed by an additional primary repair for the esophageal defect. Follow-up endoscopy demonstrated a well-repaired fistula site. The two patients were discharged without recurrence.
Our experience suggests that one step repair for atrial-esophageal fistula via lateral thoracotomy might be feasible in selected patients.
Mechanistic Analysis of Nanocellulose Formation Tuned by Deep Eutectic Solvents
Authors: Bi Xuerong , Guo Jiansheng , Wen Jin , Yu Chongwen
Published: October 2022

Clean energy and green solvents have attracted wide attention due to their non-toxic, biodegradable, and recyclable properties. Deep eutectic solvents (DESs), as a green solvent, have advantages in the formation of nanocellulose. To reveal the formation mechanism during cellulose nanocrystal (CNC) formation, different carboxylic acid DESs are compared in the optimal experimental conditions. Experimental observations show that oxalic acid (OA) DES can fabricate CNC with higher yield, higher crystalline index than that of citric acid series. Moreover, crystal water molecules in DES promote the reaction activity of DES in the CNC formation. To understand the interaction among the DES-cellulose complex, molecular dynamics simulations and quantum chemical calculations were applied to investigate the arrangement of CNC in the atomic scale. The analysis of the radial distribution function and intermolecular interactions indicate that the non-covalent intermolecular interactions between DES and cellulose are strong, which could be further enhanced by the crystal waters in DES. Reaction pathways during the formation of CNCs were revealed by computational simulations, which show that OA is more prone to react with cellulose in esterification and acidolysis reactions. Both computational and experimental results demonstrate that the OA DES is more beneficial in the production of CNC. The synergistic effects of chemical reactions and non-covalent interactions favor the formation of CNCs by DESs.
Structure and proteomic analysis of the crown-of-thorns starfish (Acanthaster planci cf. solaris) radial nerve cord
Authors: Smith Meaghan K. , Rotgans Bronwyn A. , Lang Tomas , Johnston Ryan , Wang Tianfang , Suwansa-ard Saowaros , Bose Utpal , Satoh Nori , Egertova Michaela , Hall Michael R. , Bryne Maria , Elphick Maurice R. , Motti Cherie A. , Cummins Scott F.
Published: October 2022

The nervous system of the Asteroidea (starfish or seastar) consists of radial nerve cords (RNCs) that interconnect with a ring nerve. Despite its relative simplicity, it facilitates the movement of multiple arms and numerous tube feet, as well as regeneration of damaged limbs. Here, we investigated the RNC ultrastructure and its molecular components within the of Pacific crown-of-thorns starfish (Acanthaster cf. solaris), a well-known coral predator that in high-density outbreaks has major ecological impacts on coral reefs. We describe the presence of an array of unique small bulbous bulbs (40–100 µm diameter) that project from the ectoneural region of the adult RNC. Each comprise large secretory-like cells and prominent cilia. In contrast, juvenile A. cf. solaris and its congener Acanthaster brevispinus lack these features, both of which are non-corallivorous. We hypothesise that adult neural bulbs might be an adaptation to corallivory. Proteomic analysis of the RNC (and isolated neural bulbs) provides the first comprehensive echinoderm protein database for neural tissue, including numerous secreted proteins associated with signalling, transport and defence. The neural bulbs contained several neuropeptides (e.g., bombyxin-type, starfish myorelaxant peptide, secretogranin 7B2-like, Ap15a-like, and ApNp35) and Deleted in Malignant Brain Tumor 1-like proteins. In summary, this study provides a new insight into the novel traits of A. cf. solaris, a major coral pest, and a proteomics resource that can be used to develop (bio)control strategies and understand molecular mechanisms of regeneration.