bluuebunny/crossref_metadata_2025 huggingface dataset:
Genetics of Inherited Retinal Diseases in Understudied Populations
Authors: Kannabiran Chitra , Parameswarappa Deepika , Jalali Subhadra
Published: February 2022

Retinitis pigmentosa is one of the major forms of inherited retinal dystrophy transmitted in all Mendelian and non-Mendelian forms of inheritance. It involves the loss of retinal photoreceptor cells with severe loss of vision or blindness within the first 2 decades of life. RP occurs at a relatively high prevalence in India and is often associated with consanguinity in certain South Asian communities where this practice is customary. This review describes the studies that have been published with regard to genetics of retinitis pigmentosa in India and neighboring South Asian countries. These populations have been understudied in these aspects although to a variable degree from one country to another. Genetic studies on RP in India have been carried out with a range of methods aimed at detecting specific mutations, to screening of candidate genes or selected genomic regions, homozygosity mapping to whole genome sequencing. These efforts have led to a molecular genetic characterization of RP in Indian families. Similar studies on large extended families from Pakistan have provided insight into several novel genes underlying the pathogenesis of these diseases. The extreme degree of clinical and genetic heterogeneity of RP renders it challenging to identify the associated genes in these populations, and to translate the research output towards better management of the disease, as there are no unifying genetic features that are characteristic of any population so far.
Does the Amniotic Fluid of Mice Contain a Viable Microbiota?
Authors: Winters Andrew D. , Romero Roberto , Greenberg Jonathan M. , Galaz Jose , Shaffer Zachary D. , Garcia-Flores Valeria , Kracht David J. , Gomez-Lopez Nardhy , Theis Kevin R.
Published: February 2022

The existence of an amniotic fluid microbiota (i.e., a viable microbial community) in mammals is controversial. Its existence would require a fundamental reconsideration of fetal in utero exposure to and colonization by microorganisms and the role of intra-amniotic microorganisms in fetal immune development as well as in pregnancy outcomes. In this study, we determined whether the amniotic fluid of mice harbors a microbiota in late gestation. The profiles of the amniotic fluids of pups located proximally or distally to the cervix were characterized through quantitative real-time PCR, 16S rRNA gene sequencing, and culture (N = 21 dams). These profiles were compared to those of technical controls for bacterial and DNA contamination. The load of 16S rRNA genes in the amniotic fluid exceeded that in controls. Additionally, the 16S rRNA gene profiles of the amniotic fluid differed from those of controls, with Corynebacterium tuberculostearicum being differentially more abundant in amniotic fluid profiles; however, this bacterium was not cultured from amniotic fluid. Of the 42 attempted bacterial cultures of amniotic fluids, only one yielded bacterial growth – Lactobacillus murinus. The 16S rRNA gene of this common murine-associated bacterium was not detected in any amniotic fluid sample, suggesting it did not originate from the amniotic fluid. No differences in the 16S rRNA gene load, 16S rRNA gene profile, or bacterial culture were observed between the amniotic fluids located Proximally and distally to the cervix. Collectively, these data indicate that, although there is a modest DNA signal of bacteria in murine amniotic fluid, there is no evidence that this signal represents a viable microbiota. While this means that amniotic fluid is not a source of microorganisms for in utero colonization in mice, it may nevertheless contribute to fetal exposure to microbial components. The developmental consequences of this observation warrant further investigation.
Associations Between Symptoms, Donor Characteristics and IgG Antibody Response in 2082 COVID-19 Convalescent Plasma Donors
Authors: Vinkenoog Marieke , Steenhuis Maurice , Brinke Anja ten , van Hasselt J. G. Coen , Janssen Mart P. , van Leeuwen Matthijs , Swaneveld Francis H. , Vrielink Hans , van de Watering Leo , Quee Franke , van den Hurk Katja , Rispens Theo , Hogema Boris , van der Schoot C. Ellen
Published: February 2022

Many studies already reported on the association between patient characteristics on the severity of COVID-19 disease outcome, but the relation with SARS-CoV-2 antibody levels is less clear. To investigate this in more detail, we performed a retrospective observational study in which we used the IgG antibody response from 11,118 longitudinal antibody measurements of 2,082 unique COVID convalescent plasma donors. COVID-19 symptoms and donor characteristics were obtained by a questionnaire. Antibody responses were modelled using a linear mixed-effects model. Our study confirms that the SARS-CoV-2 antibody response is associated with patient characteristics like body mass index and age. Antibody decay was faster in male than in female donors (average half-life of 62 versus 72 days). Most interestingly, we also found that three symptoms (headache, anosmia, nasal cold) were associated with lower peak IgG, while six other symptoms (dry cough, fatigue, diarrhoea, fever, dyspnoea, muscle weakness) were associated with higher IgG concentrations.
IL-17A Mediates Demyelination by Activating A1 Astrocytes via SOCS3 During Angiostrongylus cantonensis Infection
Authors: Zhou Zongpu , Lin Tuo , Liu Zhen , Ding Qian , Ma Zhixuan , Li Wanqi , Xie Fukang , Lan Yue , Feng Ying
Published: February 2022

Background

Demyelinating disease of the central nervous system is one of the most common neurological diseases and effective treatment is still under in-depth research. Our previous study showed that Angiostrongylus cantonensis infection can induce demyelination injury in mouse brains and IL-17A expression was shown to be significantly increased during this process. Moreover, we found that IL-17A inhibition attenuated the demyelination caused by A. cantonensis infection. However, the underlying mechanisms have not yet been fully elucidated.

Methods

IL-17A neutralizing antibodies were injected into A. cantonensis infected mice to decrease IL-17A levels. The activation of glial cells in the brain and the expression of cell markers were detected by a variety of methods, including real-time quantitative PCR, western blotting, and immunofluorescence staining. The relationship between IL-17A and astrocyte activation was further identified by in vitro experiments. The role of SOCS3 in the IL-17A stimulating process was determined using RNA-seq data collection of infected mice and the siRNA interference method.

Results

Demyelination of the corpus callosum was relieved after administration of IL-17A neutralizing antibody and this was accompanied by decreased activation of A1 type astrocytes around this region. The expression of SOCS3 was attenuated and activation of astrocytes by IL-17A was mediated by the IL-17RA/STAT3/SOCS3 pathway. IL-17A not only directly damaged oligodendrocytes but also indirectly damaged oligodendrocytes through A1 astrocyte mediation. Specific siRNA inhibition of IL-17A-inducible SOCS3 in astrocytes alleviated their damaging effects on oligodendrocytes.

Conclusion

IL-17A plays an important role in demyelination induced by A. cantonensis infection via the IL-17RA/STAT3/SOCS3 pathway in A1-type astrocytes, indicating that specific blockage of IL-17A and SOCS3 activity could be a therapeutic strategy for neuroinflammatory demyelinating diseases associated with astrocyte activation.
Effect of Extracted Titanium Tailing Slag on the Properties of Alkali-Activated Fly Ash-Ground Blast Furnace Slag Binder
Authors: Wang Shuping , Li Jingjing , Yun Xiaoxin , Lv Xuewei , Zhao Yujie , Zhang Zhigang
Published: February 2022

The extracted titanium tailings slag (TS) is a by-product of titanium extraction from the blast furnace slag. Its chemical composition is similar to that of ground granulated blast furnace slag (GGBS), but the relatively lower reactivity limited its utilization as a supplementary cementitious material. In this study, the reactivity of TS was improved by mechanical grinding, and the optimum property of finely ground TS was selected to replace fly ash (FA) in the alkali-activated FA-GGBS binder. The influence of TS content on the fluidity, setting time, and compressive strength of the ternary binder was investigated. X-Ray diffraction, Fourier-transform infrared spectroscopy, and scanning electron microscopy were used to analyze the effect of TS on the reaction process. Results showed that the optimum reactivity index of TS was 81.18% at 28 days when it was ground for 15 min in a ball mill. The highest compressive strength of the alkali-activated ternary binder was achieved when the replacement of FA by ground TS was 40%, and its 28-day compressive strength was 49.3 MPa, approximately 22% higher than the binder without TS. The addition of the TS would accelerate the reaction process of binder to form more products, including C-(A)-S-H and gismondine.
Irradiation Effect on F321 Austenitic Stainless-Steel: Nanoindentation and Modeling With the Crystal Plasticity Method
Authors: Liu Meidan , Nie Junfeng , Lin Pandong
Published: February 2022

In this study, 3.5 MeV Fe-ion irradiation experiments were conducted on F321 austenitic stainless-steel at different temperatures and doses. The nanohardness of the unirradiated and irradiated samples was characterized by performing nanoindentation experiments. Irradiation softening and hardening were clearly observed at 20, 100, and 300°C. However, at 300°C, after irradiation softening, the nanohardness first increased and then decreased, as opposed to the nanohardness at 20 and 100°C, which increased as the dose increased. In addition, a crystal plasticity model for a face-centered cubic single-crystal while considering irradiation-induced hardening has been proposed and numerically implemented in the user-material subroutine UMAT of ABAQUS. This was done to simulate the load-depth data of the nanoindentation experiment. The simulated results of the non-irradiated and irradiated F321 austenitic stainless-steel were compared with the experimental data. A good agreement was observed, which demonstrates the effectiveness and accuracy of the model.
Performance Optimization of Modified Gussasphalt Binder Prepared Using Natural Asphalt
Authors: Li Tao , Jin Qing , Jiang Peng , Sun Huadong , Ding Yongling , Yan Zongyao , Shi Nan
Published: February 2022

The advantageous thermal stability and construction workability of gussasphalt, a type of asphalt concrete, has drawn increasing attention. To prepare a type of superior gussasphalt concrete using Qingchuan rock asphalt (QRA), a modified gussasphalt binder was prepared with modifiers made up by different dose of styrene-butadiene-styrene, terpene resin, furfural extraction oil and SAW. Then, the rheological properties, microstructure, and thermal stability of gussasphalt binder were studied. The results indicated that the modifier #1 had significant effects on the other indexes but for the ductility, it was less effective in improving the low-temperature performance of the asphalt binder. The low-temperature performance of the binder with modifier #2 was superior compared with that of binder with modifier #1. The binder with 12.5% QRA and 5% modifier #2 can be regarded as an optimal collocation, which had a superior compatibility, high-temperature performance, and comprehensive temperature susceptibility.
Prospects of Plant-Based Trimethylolpropane Esters in the Biolubricant Formulation for Various Applications: A Review
Authors: Raof Nurliyana A. , Hamid Hamidah Abd , Mohamad Aziz Nur Atiqah , Yunus Robiah
Published: February 2022

Biodegradable lubricants from renewable feedstocks have been successfully developed to meet the demands of new machines with stringent requirements of the global standards, which address sustainability and environmental policy. Trimethylolpropane ester (TMPE) has been extensively evaluated as a biolubricant base stock and occasionally used as an additive, due to their low toxicity and excellent biodegradability. The formulation of high-performance TMPE-based lubricants involves addition of surface additives, multifunctional additives, and solid nano particles. This review focuses on the development of plant-based TMPE formulation for various applications, namely food-grade lubricant, engine oil, drilling fluid, insulating fluid, metal working fluid, hydraulic and heat transfer fluids. Even though plant-based TMPE lubricants have huge advantages over mineral oils, they have other challenging issues such as limited load-bearing capacity, hygroscopic properties, and high risk of toxic emission owing to additives selection. The details on the performance characteristics of TMPE as base stocks and additives are discussed, including the current prospects and challenges in the respective areas. This review concludes with a brief discussion on suggestions and recommendations for future advancement in the usage of TMPE and the remaining issues that must be overcome to allow for its full potential to be realized.
Construction and Evaluation of a Clinical Prediction Scoring System for Positive Cervical Margins Under Colposcopy
Authors: Zhu Meiling , Yu Mingyue , Chen Zhengzheng , Zhao Weidong
Published: February 2022

Introduction

Currently, the commonly used surgical methods for cervical lesions include loop electrosurgical excision procedure (LEEP) and cold knife conization (CKC). However, the positive rate of surgical margins after LEEP is relatively high, which leads to disease recurrence and places further demand on clinical treatment. This study investigated factors related to positive margins after LEEP and established a scoring system to enhance preoperative risk assessment and surgical selection.

Materials and Methods

A retrospective analysis of the clinical data of 411 patients undergoing LEEP surgery for cervical lesions in the First Affiliated Hospital of University of Science and Technology of China (USTC), from January 2016 to March 2021, was performed. Cases were divided into a negative margin group (349 cases) and a positive margin group according to postoperative pathology. In the positive group (62 cases), single-factor and multi-factor analyses screened influencing factors; a logistic and additive scoring system was established; furthermore, a ROC curve was used to evaluate scoring effectiveness.

Results

The positive rate of resection margins after LEEP was 15.1%. Univariate analysis indicated a relationship to patient age, menopause, preoperative ThinPrep Cytology Test (TCT) results, lesion quadrant number under colposcopy, cervical biopsy, and the result of endocervical curettage (ECC). Multivariate analysis showed that age >35 y, menopause, preoperative TCT being high-grade squamous intraepithelial lesion (HSIL), four quadrants being involved under colposcopy, and ECC being HSIL were all independent influencing factors of positive margins after LEEP (P < 0.05). These were included with the above factors to establish a logistic and additive scoring system. When the logistic score was 17, the sensitivity and specificity of predicting positive margins after LEEP were 80.6 and 61.6%, respectively. When the additive score was 6, the sensitivity and specificity were 74.2 and 66.2%, respectively. Both scoring systems had good predictability (area under the curve AUC >0.75).

Conclusions

This study quantified factors influencing positive margins after LEEP and established a scoring system for evaluating patients before surgery to provide a basis for individualized treatment and selection of surgical methods.
In vitro Evaluation of the Anti-hypercholesterolemic Effect of Lactobacillus Isolates From Various Sources
Authors: Tjandrawinata Raymond Rubianto , Kartawijaya Medicia , Hartanti Apriliana Wahyu
Published: February 2022

The anti-hypercholesterolemic effect of 11 Lactobacillus isolates was investigated in vitro by measuring remaining cholesterol in growth media, growth ability in media supplemented with cholesterol, and BSH activity. Among the selected isolates, DLBSH104, DLBSH122, and DLBSK207 have demonstrated outstanding potential as cholesterol-lowering cultures. The three isolates showed high cholesterol removal by growing cells, whereas resting and dead cells showed less cholesterol removal. Furthermore, visualization of those isolates in growing and non-growing states by SEM showed the ability of DLBSH104 to attach cholesterol to their cell surface. In contrast, alteration of DLBSH122 and DLBSK207 cells did not involve surface attachment of cholesterol. Thus, the isolates’ ability to remove cholesterol is mainly attributed to the cells’ metabolically active state that assimilates and incorporates cholesterol into the cell membrane as reflected by a significantly higher cholesterol removal in a growing state than a non-growing state. Only in DLBSH104 did cholesterol removal also involve attachment on the cell surface. Moreover, DLBSH104 has beneficially affected the host cell by a significant reduction of NPC1L1 mRNA levels that are responsible for intestinal cholesterol absorption. In hepatic cells, cell-free supernatant (CFS) from DLBSH104 and DLBSK207 were able to reduce LDLR and HMGCR mRNA at the transcription level. To sum up, L. helveticus DLBSH104 and L. plantarum DLBSK207 are confirmed as isolates with an anti-hypercholesterolemic effect.
Characterization of the Trimethylamine N-Oxide Transporter From Pelagibacter Strain HTCC1062 Reveals Its Oligotrophic Niche Adaption
Authors: Gao Chao , Zhang Nan , He Xiao-Yan , Wang Ning , Zhang Xi-Ying , Wang Peng , Chen Xiu-Lan , Zhang Yu-Zhong , Ding Jun-Mei , Li Chun-Yang
Published: February 2022

Trimethylamine N-oxide (TMAO), which was detected at nanomolar concentrations in surface seawaters, is an important carbon, nitrogen and/or energy source for marine bacteria. It can be metabolized by marine bacteria into volatile methylated amines, the second largest source of nitrogen after N2 gas in the oceans. The SAR11 bacteria are the most abundant oligotrophic plankton in the oceans, which represents approximately 30% of the bacterial cells in marine surface waters. Genomic analysis suggested that most SAR11 bacteria possess an ATP-binding cassette transporter TmoXWV that may be responsible for importing TMAO. However, it was still unclear whether SAR11 bacteria can utilize TMAO as the sole nitrogen source and how they import TMAO. Here, our results showed that Pelagibacter strain HTCC1062, a SAR11 bacterium, can grow with TMAO as the sole nitrogen source. TmoXWV from strain HTCC1062 (TmoXWV1062) was verified to be a functional TMAO importer. Furthermore, TmoX1062, the periplasmic substrate binding protein of TmoXWV1062, was shown to have high binding affinities toward TMAO at 4°C (Kd = 920 nM), 10°C (Kd = 500 nM) and 25°C (Kd = 520 nM). The high TMAO binding affinity and strong temperature adaptability of TmoX1062 reveal a possible oligotrophic niche adaptation strategy of strain HTCC1062, which may help it gain a competitive advantage over other bacteria. Structure comparison and mutational analysis indicated that the TMAO binding mechanism of TmoX1062 may have differences from the previously reported mechanism of TmoX of Ruegeria pomeroyi DSS-3. This study provides new insight into TMAO utilization by the widespread SAR11 bacteria.
The Systemic Inflammation Response Index as an Independent Predictor of Survival in Breast Cancer Patients: A Retrospective Study
Authors: Zhu Mengliu , Chen Li , Kong Xiangyi , Wang Xiangyu , Fang Yi , Li Xingrui , Wang Jing
Published: February 2022

There is a close relationship between inflammatory cells and tumors, but the pathways that connect the two remain unclear. This research explores the clinical and prognostic value of the systemic inflammation response index (SIRI) in breast cancer patients. The study included 477 breast cancer patients who underwent neoadjuvant chemotherapy and 308 breast cancer patients who did not in our center between January 1998 and December 2016. Optimal SIRI threshold values were determined using the receiver operating characteristic curve (ROC). Patients were then reclassified as SIRI ≥0.80 group (High SIRI group) and SIRI <0.80 group (Low SIRI group). The outcomes were analyzed by statistical methods. The univariate and multivariate analyses demonstrated that SIRI independently predicted survival in breast cancer. The disease-free survival (DFS) and overall survival (OS) in patients with low SIRI scores were significantly longer in contrast to those with high SIRI scores (41.50 vs. 37.63 months, and 64.57 vs. 58.42 months). Further subgroup analyses revealed that low SIRI score patients who also had either early breast cancer, advanced breast cancer, or different molecular subtypes also possessed longer mean survival time of DFS and OS in contrast to those with high SIRI levels (χ2 = 2.379, p = 0.123, and χ2 = 5.153, p = 0.023; χ2 = 11.080, p = 0.0009 and χ2 = 15.900, p < 0.0001; χ2 = 16.020, p < 0.0001 and χ2 = 22.050, p < 0.0001, respectively). SIRI serves as an easily accessible, replicable, and minimally invasive prognostic tool in breast cancer patients. Lower SIRI scores were predictive of a longer DFS and OS after surgery in breast cancer patients. SIRI may serve as a marker to guide clinical management and prognostication of breast cancer.
Forgiveness Mediates the Relationship Between Middle Frontal Gyrus Volume and Clinical Symptoms in Adolescents
Authors: Schuttenberg Eleanor M. , Sneider Jennifer T. , Rosmarin David H. , Cohen-Gilbert Julia E. , Oot Emily N. , Seraikas Anna M. , Stein Elena R. , Maksimovskiy Arkadiy L. , Harris Sion K. , Silveri Marisa M.
Published: February 2022

Dispositional forgiveness is positively associated with many facets of wellbeing and has protective implications against depression and anxiety in adolescents. However, little work has been done to examine neurobiological aspects of forgiveness as they relate to clinical symptoms. In order to better understand the neural mechanisms supporting the protective role of forgiveness in adolescents, the current study examined the middle frontal gyrus (MFG), which comprises the majority of the dorsolateral prefrontal cortex (DLPFC) and is associated with cognitive regulation, and its relationship to forgiveness and clinical symptoms in a sample of healthy adolescents. In this cross-sectional study (n = 64), larger MFG volume was significantly associated with higher self-reported dispositional forgiveness scores and lower levels of depressive and anxiety symptoms. Forgiveness mediated the relationship between MFG volume and both depressive and anxiety symptom levels. The mediating role of forgiveness in the relationship between MFG volume and clinical symptoms suggests that one way that cognitive regulation strategies supported by this brain region may improve adolescent mental health is via increasing a capacity for forgiveness. The present study highlights the relevance of forgiveness to neurobiology and their relevance to emotional health in adolescents. Future longitudinal studies should focus on the predictive quality of the relationship between forgiveness, brain volume and clinical symptoms and the effects of forgiveness interventions on these relationships.
Functional Implications of Dale's Law in Balanced Neuronal Network Dynamics and Decision Making
Authors: Barranca Victor J. , Bhuiyan Asha , Sundgren Max , Xing Fangzhou
Published: February 2022

The notion that a neuron transmits the same set of neurotransmitters at all of its post-synaptic connections, typically known as Dale's law, is well supported throughout the majority of the brain and is assumed in almost all theoretical studies investigating the mechanisms for computation in neuronal networks. Dale's law has numerous functional implications in fundamental sensory processing and decision-making tasks, and it plays a key role in the current understanding of the structure-function relationship in the brain. However, since exceptions to Dale's law have been discovered for certain neurons and because other biological systems with complex network structure incorporate individual units that send both positive and negative feedback signals, we investigate the functional implications of network model dynamics that violate Dale's law by allowing each neuron to send out both excitatory and inhibitory signals to its neighbors. We show how balanced network dynamics, in which large excitatory and inhibitory inputs are dynamically adjusted such that input fluctuations produce irregular firing events, are theoretically preserved for a single population of neurons violating Dale's law. We further leverage this single-population network model in the context of two competing pools of neurons to demonstrate that effective decision-making dynamics are also produced, agreeing with experimental observations from honeybee dynamics in selecting a food source and artificial neural networks trained in optimal selection. Through direct comparison with the classical two-population balanced neuronal network, we argue that the one-population network demonstrates more robust balanced activity for systems with less computational units, such as honeybee colonies, whereas the two-population network exhibits a more rapid response to temporal variations in network inputs, as required by the brain. We expect this study will shed light on the role of neurons violating Dale's law found in experiment as well as shared design principles across biological systems that perform complex computations.
Feasibility of Preserving No. 5 and No. 6 Lymph Nodes in Gastrectomy of Proximal Gastric Adenocarcinoma: A Retrospective Analysis of 395 Patients
Authors: Yang Xiao , Zheng Yanan , Feng Runhua , Zhu Zhenggang , Yan Min , Li Chen
Published: February 2022

Objective

The extent of regional lymphadenectomy for proximal gastric cancer (PGC) has remained a controversy and a matter of considerable debate for a long time. We retrospectively analyzed the clinicopathological features to investigate the predictive factors for No. 5 and/or No. 6 lymph node metastases (LNMs) and evaluate the feasibility of performing proximal gastrectomy (PG) with preservation of No. 5 and/or No. 6 lymph nodes for these patients.

Method

Patients who had undergone total gastrectomy plus D2 lymphadenectomy in the Department of Gastrointestinal Surgery, Ruijin Hospital, Shanghai Jiao Tong University, School of Medicine, from January 2008 to December 2017 were retrospectively collected and analyzed.

Results

Among the 395 eligible patients in our study, 34 patients (8.61%) had No. 5 and No. 6 LNM. The degree of differentiation, Borrmann classification, vascular or perineural invasion, tumor diameter, depth of invasion, and other perigastric LNM were associated with No. 5 and/or No. 6 LNM. Multivariate analyses showed that tumor diameter ≥4 cm, No. 4 LNM positive, and No. 7, No. 8, No. 9 LNM positive were independent risk factors of No. 5 and/or No. 6 LNM. No. 5 and/or No. 6 LNM was not observed in the 105 patients who were staged from T1 to T3 and were found to be without independent risk factors.

Conclusion

The metastatic rate of No. 5 and/or No. 6 lymph node of the proximal gastric adenocarcinoma was closely associated with the diameter of the tumor and other perigastric LNMs. It is feasible to preserve No. 5 and No. 6 lymph nodes with PG for the T1–T3 patients with lower risk of No. 5 and/or No. 6 LNM.
Assessment of Primary Colorectal Cancer CT Radiomics to Predict Metachronous Liver Metastasis
Authors: Li Yue , Gong Jing , Shen Xigang , Li Menglei , Zhang Huan , Feng Feng , Tong Tong
Published: February 2022

Objectives

To establish and validate a machine learning-based CT radiomics model to predict metachronous liver metastasis (MLM) in patients with colorectal cancer.

Methods

In total, 323 patients were retrospectively recruited from two independent institutions to develop and evaluate the CT radiomics model. Then, 1288 radiomics features were extracted to decode the imaging phenotypes of colorectal cancer on CT images. The optimal radiomics features were selected using a recursive feature elimination selector configured by a support vector machine. To reduce the bias caused by an unbalanced dataset, the synthetic minority oversampling technique was applied to resample the minority samples in the datasets. Then, both radiomics and clinical features were used to train the multilayer perceptron classifier to develop two classification models. Finally, a score-level fusion model was developed to further improve the model performance.

Results

The area under the curve (AUC) was 0.78 ± 0.07 for the tumour feature model and 0.79 ± 0.08 for the clinical feature model. The fusion model achieved the best performance, with AUCs of 0.79 ± 0.08 and 0.72 ± 0.07 in the internal and external validation cohorts.

Conclusions

Radiomics models based on baseline colorectal contrast-enhanced CT have high potential for MLM prediction. The fusion model combining radiomics and clinical features can provide valuable biomarkers to identify patients with a high risk of colorectal liver metastases.
Factors Associated With Neural Tube Defects Among Newborns Delivered at Debre Berhan Specialized Hospital, North Eastern Ethiopia, 2021. Case-Control Study
Authors: Mulu Getaneh Baye , Atinafu Bantalem Tilaye , Tarekegn Fetene Nigussie , Adane Tigist Demssew , Tadese Mesfin , Wubetu Abate Dargie , Kebede Worku Misganaw
Published: February 2022

Background

Neural tube defects are severe congenital malformations secondary to an abnormal closure of the neural tube between third and fourth weeks of gestational ages. Neural tube defects affect birth outcomes worldwide, with an occurrence of 18.6 per 10,000 live births. In addition, neural tube defects are associated with considerable mortality, morbidity, disability, and socio-economical cost.

Objective

To identify factors associated with neural tube defects among newborns delivered at Debre Berhan Comprehensive Specialized Hospital, 2021.

Methods

Facility-based case-control study design was conducted among 381 (127 cases and 254 controls) newborns delivered from June 2019 to June 2021 at Debre Berhan Specialized Hospital. Consecutive and systematic random sampling techniques were used to select cases and controls, respectively. Data were collected using semi-structured checklists. Finally, data were entered using Epidata version 4.2.1 and analyzed using SPSS version 25. In the bivariable logistic regression model, factors with a p < 0.20 were entered into multivariable logistic regressions. Statistical significance was declared at a p < 0.05.

Result

In this study, 381 newborns (127 cases and 254 controls) participated with a response rate of 100%. In the logistic regression model, mothers who took medication during pregnancy [AOR 1.83 (95% CI 1.08–3.08)], mothers who did not take a balanced diet during pregnancy [AOR 13.46 (95% CI 7.83–23.13)], and mothers who did not take folic acid before and during the first trimester of pregnancy [AOR 1.71 (95% CI 1.01–2.94)] were significantly associated with neural tube defect.

Conclusion and Recommendation

Mothers who took medication during pregnancy, mothers who did not take balanced diets during pregnancy, and mothers who did not take folic acid during pregnancy were the significant factors of neural tube defects. Health care professionals should focus on maternal safe drug prescription, maternal folate intake, and a balanced diet before and during pregnancy.
Agreement Between Caregivers' Concerns of Children's Developmental Problems and Professional Identification in Taiwan
Authors: Lin Ling-Yi , Yu Wen-Hao , Lin Wei-Pin , Chen Chih-Chia , Tu Yi-Fang
Published: February 2022

Objective

Early detection of developmental delays relies on the accuracy of the caregivers' concerns of children's developmental problems. The aim of this study was to investigate the agreement between the caregivers' awareness of children's developmental problems and professional identification.

Methods

Caregivers of 1,963 children (age range: 5–71 months; mean: 38.4 months) younger than 6 years old who were at risk of developmental delays and referred to the center for a comprehensive evaluation were enrolled in this study. Children were identified by a transdisciplinary team including a pediatric neurologist, a pediatric psychiatrist, two psychologists, two occupational therapists, two physical therapists, two speech therapists, a social worker, and a special instructor. A series of standardized developmental assessments were used to identify children with developmental delay. Retrospective chart reviews were conducted on all children to confirm specific developmental disorders.

Results

The caregivers' initial concerns of cognitive, speech/language, emotional/behavioral, and motor and global development showed low agreement with the results of professional identification. The major disagreement was observed in the cognitive domain. Speech/language developmental concern was an important red-flag indicator of cognitive and emotional/behavioral developmental delays. The presence of intellectual disability, autism spectrum disorder, and attention deficit hyperactivity disorder was high in this study. When having caregivers' concerns about speech/language and emotional/behavioral development, the odds of children with autism spectrum disorder was 2.37 and 2.17 times greater than those without autism spectrum disorder, respectively. The presence of attention deficit hyperactivity disorder was significantly associated with concerns about cognitive and emotional/behavioral developmental delays. Child's age and mothers' level of education were significant indicators for detecting the child's developmental problems.

Conclusion

It is recommended that assessing the cognitive developmental status is essential for all children in the identification process. Practitioners should not overlook caregivers' concern about speech/language and emotional/behavioral development. Transdisciplinary practitioners provide educational guidance to caregivers, especially in the domains of cognitive, speech/language, and emotional/behavioral development.
ECHOPAEDIA: Echography in Paediatric Patients in the Age of Coronavirus Disease 2019: Utility of Lung Ultrasound and Chest X-Ray in Diagnosis of Community-Acquired Pneumonia and Severe Acute Respiratory Syndrome Coronavirus 2 Pneumonia
Authors: Fiorito Ivan , Gori Giulia , Perrone Tiziano , Mascolo Amelia , Caimmi Silvia , Palumbo Ilaria , De Silvestri Annalisa , Delliponti Mariangela , Di Sabatino Antonio , Marseglia Gian Luigi
Published: February 2022

Background

In recent years, lung ultrasound (LUS) has spread to emergency departments and clinical practise gaining great support, especially in time of pandemic, but only a few studies have been done on children. The aim of the present study is to compare the diagnostic accuracy of LUS (using Soldati LUS score) and that of chest X-ray (CXR) in CAP and COVID-19 pneumonia in paediatric patients. Secondary objective of the study is to examine the association between LUS score and disease severity. Finally, we describe the local epidemiology of paediatric CAP during the study period in the era of COVID-19 by comparing it with the previous 2 years.

Methods

This is an observational retrospective single-centre study carried out on patients aged 18 or younger and over the month of age admitted to the Paediatric Unit of our Foundation for suspected community-acquired pneumonia or SARS-CoV-2 pneumonia during the third pandemic wave of COVID-19. Quantitative variables were elaborated with Shapiro–Wilks test or median and interquartile range (IQR). Student's t-test was used for independent data. Association between quantitative data was evaluated with Pearson correlation. ROC curve analysis was used to calculate best cut-off of LUS score in paediatric patients. Area under the ROC curve (AUC), sensibility, and specificity are also reported with 95% confidence interval (CI).

Results

The diagnostic accuracy of the LUS score in pneumonia, the area underlying the ROC curve (AUC) was 0.67 (95% CI: 0.27–1) thus showing a discrete discriminatory power, with a sensitivity of 89.66% and specificity 50% setting a LUS score greater than or equal to 1 as the best cut-off. Nine patients required oxygen support and a significant statistical correlation (p = 0.0033) emerged between LUS score and oxygen therapy. The mean LUS score in patients requiring oxygen therapy was 12. RCP was positively correlated to the patient's LUS score (p = 0.0024).

Conclusions

Our study has shown that LUS is a valid alternative to CXR. Our results show how LUS score can be applied effectively for the diagnosis and stratification of paediatric pneumonia.
Spontaneous Resolution of Congenital Dural Venous Sinus Ectasia Associated With Polymicrogyria—Case Report
Authors: Kozłowska Zuzanna , Komasińska Paulina , Steinborn Barbara , Toboła-Wróbel Kinga , Pietryga Marek , Szymankiewicz-Breborowicz Marta , Szczapa Tomasz , Bekiesińska-Figatowska Monika
Published: February 2022

Dural venous sinus ectasia belongs to a rare group of venous sinus malformations of unknown origin and uncertain prognosis. We report the first patient with idiopathic congenital ectasia of the confluence of sinuses with thrombosis associated with bilateral polymicrogyria. It may highlight the causative relation between ischemia within the central nervous system due to torcular herophili ectasia with thrombosis in early pregnancy and the development of cortical malformations in neonates. We also highlight the role of MR neuroimaging in the diagnosis of these entities.
Critical Damage and Microstructure Characteristics of Sandstone in the Three Gorges Reservoir Area by Single Specimen Method Under Different Stress Paths
Authors: Li Hua , Wang ZhiPeng , Dai Lingquan , Wan Liangpeng , Deng Huafeng , Yang Chao , Chen Jianghong , Pan Hongyue , Wang Lei , Jiang Qiao
Published: February 2022

The critical damage value of rock is an important parameter of stability analysis when engineering rock mass. Based on the previous single test method, this paper, combined with the deformation characteristics of rock residual phase under different stress paths, proposed a single specimen repeated loading method to measure the critical damage value of rock based on different damage variables. The paper also proposed to modify the critical damage value based on residual constitutive energy and to improve the definition of damage variables based on constitutive energy dissipation. According to the microstructure characteristics of the rock sample, the mechanism of the single specimen method to determine the critical value of rock sample damage is revealed. The results show that: 1) Comparing the results of triaxial failure test of rock under different stress paths and single specimen repeated loading test, the residual strength of rock sample is mainly controlled by the confining pressure of loading, which is not an obvious relationship with initial confining pressure and stress path; 2) The number of repeated compressions has little effect on the internal structure, particle flatness, and particle surface smoothness of their specimens, which is reflected at the macroscopic level that the residual strength of sandstone tends to achieve a stable value; and 3) The corrected critical damage value based on residual strain energy is closer to the theoretical value. Test methods in this paper can provide useful references for determination of critical damage value.
Insights Into the Peroxisomal Protein Inventory of Zebrafish
Authors: Kamoshita Maki , Kumar Rechal , Anteghini Marco , Kunze Markus , Islinger Markus , Martins dos Santos Vítor , Schrader Michael
Published: February 2022

Peroxisomes are ubiquitous, oxidative subcellular organelles with important functions in cellular lipid metabolism and redox homeostasis. Loss of peroxisomal functions causes severe disorders with developmental and neurological abnormalities. Zebrafish are emerging as an attractive vertebrate model to study peroxisomal disorders as well as cellular lipid metabolism. Here, we combined bioinformatics analyses with molecular cell biology and reveal the first comprehensive inventory of Danio rerio peroxisomal proteins, which we systematically compared with those of human peroxisomes. Through bioinformatics analysis of all PTS1-carrying proteins, we demonstrate that D. rerio lacks two well-known mammalian peroxisomal proteins (BAAT and ZADH2/PTGR3), but possesses a putative peroxisomal malate synthase (Mlsl) and verified differences in the presence of purine degrading enzymes. Furthermore, we revealed novel candidate peroxisomal proteins in D. rerio, whose function and localisation is discussed. Our findings confirm the suitability of zebrafish as a vertebrate model for peroxisome research and open possibilities for the study of novel peroxisomal candidate proteins in zebrafish and humans.
Green Visual Sensor of Plant: An Energy-Efficient Compressive Video Sensing in the Internet of Things
Authors: Li Ran , Yang Yihao , Sun Fengyuan
Published: February 2022

Internet of Things (IoT) realizes the real-time video monitoring of plant propagation or growth in the wild. However, the monitoring time is seriously limited by the battery capacity of the visual sensor, which poses a challenge to the long-working plant monitoring. Video coding is the most consuming component in a visual sensor, it is important to design an energy-efficient video codec in order to extend the time of monitoring plants. This article presents an energy-efficient Compressive Video Sensing (CVS) system to make the visual sensor green. We fuse a context-based allocation into CVS to improve the reconstruction quality with fewer computations. Especially, considering the practicality of CVS, we extract the contexts of video frames from compressive measurements but not from original pixels. Adapting to these contexts, more measurements are allocated to capture the complex structures but fewer to the simple structures. This adaptive allocation enables the low-complexity recovery algorithm to produce high-quality reconstructed video sequences. Experimental results show that by deploying the proposed context-based CVS system on the visual sensor, the rate-distortion performance is significantly improved when comparing it with some state-of-the-art methods, and the computational complexity is also reduced, resulting in a low energy consumption.
Quality of Life and Community Wellbeing of Members Associated With Village Savings and Loans Associations as a Model of Sharing Economy in the Least Developing Countries: A Case of Mzuzu City in Northern Malawi, Southern Africa
Authors: Wu Xue-Lian , Munthali George N. Chidimbah , Dzimbiri Mastano N. Woleson , Aakash Abdur Rahman , Rizwan Muhammad , Shi Yu , Daru Gama Rivas , Sheferaw Wegayehu Enbeyle
Published: February 2022

This study was aimed at examining the impacts of the Sharing economy on the individual and community Quality of Life (QOL) and wellbeing by looking at their associated influencing factors using Village Savings and Loans Associations as a model of sharing economy in Malawi. An online community-based cross-sectional study design was conducted from November 2020 through January 2021. In the survey, 402 Village Savings and Loans Associations (VSLAs) members from the Mzuzu City area participated, recruited using snowball and respondent-driven sampling techniques. The sample size was computed using a single population proportion using the Yamane formula. Descriptive statistics and ordinal logistic regression model techniques were also employed. Additionally, we used the Chi-Square test, two-way ANOVA, and Ordinal regression model to determine statistical associations between socioeconomic data and QOL and wellbeing variables with a 5% level of significance. On the aspect of community wellbeing, the findings of our study indicated that income (levels and disposal) provided members with options to live a better QOL and wellbeing within the community by either facilitating payment for better education, eating healthier foods, acquiring assets, etc. Further, the absence of discrimination provided a platform for voice, inclusion, and social trust, enhancing freedom of expression. We also found that education facilitated better earnings and knowledge of public health-related issues. As for the contribution to the sharing economy, our study has emphasized the role played by trust in enhancing sharing economy. We recommend and encourage people to join these VSLAs so as to improve their QOL and wellbeing. However, there is a need to replicate the study on a larger scale to validate our study findings for effective policy formulation and implementation geared to improving the overall quality of people’s lives. Based on these findings, we further recommend that authorities reinstate programs like National Strategy for Financial Inclusion 2016–2020 and Savings and Loan Groups Best Practice Guidelines (SLG BPGs) 2016–2017 that could further enhance the future of VSLAs, which are vital for QOL and community wellbeing of the people in developing countries like Malawi.
Incongruence in Lighting Impairs Face Identification
Authors: Lim Denise Y. , Lee Alan L. F. , Or Charles C.-F.
Published: February 2022

The effect of uniform lighting on face identity processing is little understood, despite its potential influence on our ability to recognize faces. Here, we investigated how changes in uniform lighting level affected face identification performance during face memory tests. Observers were tasked with learning a series of faces, followed by a memory test where observers judged whether the faces presented were studied before or novel. Face stimuli were presented under uniform bright or dim illuminations, and lighting across the face learning and the memory test sessions could be the same (“congruent”) or different (“incongruent”). This led to four experimental conditions: (1) Bright/Dim (learning bright faces, testing on dim faces); (2) Bright/Bright; (3) Dim/Bright; and (4) Dim/Dim. Our results revealed that incongruent lighting levels across sessions (Bright/Dim and Dim/Bright) significantly reduced sensitivity (d’) to faces and introduced conservative biases compared to congruent lighting levels (Bright/Bright and Dim/Dim). No significant differences in performance were detected between the congruent lighting conditions (Bright/Bright vs. Dim/Dim) and between the incongruent lighting conditions (Bright/Dim vs. Dim/Bright). Thus, incongruent lighting deteriorated performance in face identification. These findings implied that the level of uniform lighting should be considered in an illumination-specific face representation and potential applications such as eyewitness testimony.
Change Blindness in Adolescents With Attention-Deficit/Hyperactivity Disorder: Use of Eye-Tracking
Authors: Hochhauser Michal , Aran Adi , Grynszpan Ouriel
Published: February 2022

Objective

This study investigated change detection of central or marginal interest in images using a change-blindness paradigm with eye tracking.

Method

Eighty-four drug-naïve adolescents [44 with attention-deficit/hyperactivity disorder (ADHD)/40 controls with typical development] searched for a change in 36 pairs of original and modified images, with an item of central or marginal interest present or absent, presented in rapid alternation. Collected data were detection rate, response time, and gaze fixation duration, latency, and dispersion data.

Results

Both groups' change-detection times were similar, with no speed–accuracy trade-off. No between-group differences were found in time to first fixation, fixation duration, or scan paths. Both groups performed better for items of central level of interest. The ADHD group demonstrated greater fixation dispersion in scan paths for central- and marginal-interest items.

Conclusion

Results suggest the greater gaze dispersion may lead to greater fatigue in tasks that require longer attention duration.