bluuebunny/crossref_metadata_2025 huggingface dataset:
Effective Detection and Monitoring of Glioma Using [18F]FPIA PET Imaging
Authors: Vassileva Vessela , Braga Marta , Barnes Chris , Przystal Justyna , Ashek Ali , Allott Louis , Brickute Diana , Abrahams Joel , Suwan Keittisak , Carcaboso Angel M. , Hajitou Amin , Aboagye Eric O.
Published: July 2021

Background: Reprogrammed cellular metabolism is a cancer hallmark. In addition to increased glycolysis, the oxidation of acetate in the citric acid cycle is another common metabolic phenotype. We have recently developed a novel fluorine-18-labelled trimethylacetate-based radiotracer, [18F]fluoro-pivalic acid ([18F]FPIA), for imaging the transcellular flux of short-chain fatty acids, and investigated whether this radiotracer can be used for the detection of glioma growth. Methods: We evaluated the potential of [18F]FPIA PET to monitor tumor growth in orthotopic patient-derived (HSJD-GBM-001) and cell line-derived (U87, LN229) glioma xenografts, and also included [18F]FDG PET for comparison. We assessed proliferation (Ki-67) and the expression of lipid metabolism and transport proteins (CPT1, SLC22A2, SLC22A5, SLC25A20) by immunohistochemistry, along with etomoxir treatment to provide insights into [18F]FPIA uptake. Results: Longitudinal PET imaging showed gradual increase in [18F]FPIA uptake in orthotopic glioma models with disease progression (p < 0.0001), and high tumor-to-brain contrast compared to [18F]FDG (p < 0.0001). [18F]FPIA uptake correlated positively with Ki-67 (p < 0.01), SLC22A5 (p < 0.001) and SLC25A20 (p = 0.001), and negatively with CPT1 (p < 0.01) and SLC22A2 (p < 0.01). Etomoxir reduced [18F]FPIA uptake, which correlated with decreased Ki-67 (p < 0.05). Conclusions: Our findings support the use of [18F]FPIA PET for the detection and longitudinal monitoring of glioma, showing a positive correlation with tumor proliferation, and suggest transcellular flux-mediated radiotracer uptake.
Delayed Contralateral Nephrectomy Halted Post-Ischemic Renal Fibrosis Progression and Inhibited the Ischemia-Induced Fibromir Upregulation in Mice
Authors: Róka Beáta , Tod Pál , Kaucsár Tamás , Bukosza Éva Nóra , Vörös Imre , Varga Zoltán V. , Petrovich Balázs , Ágg Bence , Ferdinandy Péter , Szénási Gábor , Hamar Péter
Published: July 2021

(1) Background: Ischemia reperfusion (IR) is the leading cause of acute kidney injury (AKI) and results in predisposition to chronic kidney disease. We demonstrated that delayed contralateral nephrectomy (Nx) greatly improved the function of the IR-injured kidney and decelerated fibrosis progression. Our aim was to identify microRNAs (miRNA/miR) involved in this process. (2) Methods: NMRI mice were subjected to 30 min of renal IR and one week later to Nx/sham surgery. The experiments were conducted for 7–28 days after IR. On day 8, multiplex renal miRNA profiling was performed. Expression of nine miRNAs was determined with qPCR at all time points. Based on the target prediction, plexin-A2 and Cd2AP were measured by Western blot. (3) Results: On day 8 after IR, the expression of 20/1195 miRNAs doubled, and 9/13 selected miRNAs were upregulated at all time points. Nx reduced the expression of several ischemia-induced pro-fibrotic miRNAs (fibromirs), such as miR-142a-duplex, miR-146a-5p, miR-199a-duplex, miR-214-3p and miR-223-3p, in the injured kidneys at various time points. Plexin-A2 was upregulated by IR on day 10, while Cd2AP was unchanged. (4) Conclusion: Nx delayed fibrosis progression and decreased the expression of ischemia-induced fibromirs. The protein expression of plexin-A2 and Cd2AP is mainly regulated by factors other than miRNAs.
The Dual Role of a Polyvalent IgM/IgA-Enriched Immunoglobulin Preparation in Activating and Inhibiting the Complement System
Authors: Schmidt Carolin , Weißmüller Sabrina , Bohländer Fabian , Germer Matthias , König Martin , Staus Alexander , Wartenberg-Demand Andrea , Heinz Corina C. , Schüttrumpf Jörg
Published: July 2021

Activation of the complement system is important for efficient clearance of a wide variety of pathogens via opsonophagocytosis, or by direct lysis via complement-dependent cytotoxicity (CDC). However, in severe infections dysregulation of the complement system contributes to hyperinflammation. The influence of the novel IgM/IgA-enriched immunoglobulin preparation trimodulin on the complement pathway was investigated in in vitro opsonophagocytosis, binding and CDC assays. Immunoglobulin levels before and after trimodulin treatment were placed in relation to complement assessments in humans. In vitro, trimodulin activates complement and induces opsonophagocytosis, but also interacts with opsonins C3b, C4b and anaphylatoxin C5a in a concentration-dependent manner. This was not observed for standard intravenous IgG preparation (IVIg). Accordingly, trimodulin, but not IVIg, inhibited the downstream CDC pathway and target cell lysis. If applied at a similar concentration range in healthy subjects, trimodulin treatment resulted in C3 and C4 consumption in a concentration-dependent manner, which was extended in patients with severe community-acquired pneumonia. Complement consumption is found to be dependent on underlying immunoglobulin levels, particularly IgM, pinpointing their regulative function in humans. IgM/IgA provide a balancing effect on the complement system. Trimodulin may enhance phagocytosis and opsonophagocytosis in patients with severe infections and prevent excessive pathogen lysis and release of harmful anaphylatoxins.
The Effects of Obesity on Lymphatic Pain and Swelling in Breast Cancer Patients
Authors: Fu Mei Rosemary , Axelrod Deborah , Guth Amber , McTernan Melissa L. , Qiu Jeanna M. , Zhou Zhuzhu , Ko Eunjung , Magny-Normilus Cherlie , Scagliola Joan , Wang Yao
Published: July 2021

Lymphatic pain and swelling due to lymph fluid accumulation are the most common and debilitating long-term adverse effects of cancer treatment. This study aimed to quantify the effects of obesity on lymphatic pain, arm, and truncal swelling. Methods: A sample of 554 breast cancer patients were enrolled in the study. Body mass index (BMI), body fat percentage, and body fat mass were measured using a bioimpedance device. Obesity was defined as a BMI ≥ 30 kg/m2. The Breast Cancer and Lymphedema Symptom Experience Index was used to measure lymphatic pain, arm, and truncal swelling. Multivariable logistic regression models were used to estimate the odds ratio (OR) with 95% confidence interval (CI) to quantify the effects of obesity. Results: Controlling for clinical and demographic characteristics as well as body fat percentage, obesity had the greatest effects on lymphatic pain (OR 3.49, 95% CI 1.87–6.50; p < 0.001) and arm swelling (OR 3.98, 95% CI 1.82–4.43; p < 0.001). Conclusions: Obesity is a significant risk factor for lymphatic pain and arm swelling in breast cancer patients. Obesity, lymphatic pain, and swelling are inflammatory conditions. Future study should explore the inflammatory pathways and understand the molecular mechanisms to find a cure.
Sevoflurane and Desflurane Exposures Following Aneurysmal Subarachnoid Hemorrhage Confer Multifaceted Protection against Delayed Cerebral Ischemia
Authors: Jayaraman Keshav , Liu Meizi , Zipfel Gregory J. , Athiraman Umeshkumar
Published: July 2021

Numerous studies have demonstrated the ability of isoflurane conditioning to provide multifaceted protection against aneurysmal subarachnoid hemorrhage (SAH)-associated delayed cerebral ischemia (DCI); however, preclinical studies have not yet examined whether other commonly used inhalational anesthetics in neurological patients such as sevoflurane or desflurane are also protective against SAH-induced neurovascular deficits. We therefore sought to identify the potential for sevoflurane and desflurane conditioning to protect against DCI in an endovascular perforation mouse model of SAH. Neurological function was assessed daily via neuroscore. Large artery vasospasm and microvessel thrombosis were assessed three days after SAH or sham surgery. Four groups were examined: Sham, SAH + room air, SAH + 2% Sevoflurane, and SAH + 6% Desflurane. For the SAH groups, one hour after surgery, mice received 2% sevoflurane, 6% desflurane, or room air for one hour. We found that conditioning with sevoflurane or desflurane attenuated large artery vasospasm, reduced microvessel thrombosis, and improved neurologic function. Given their frequent clinical use and strong safety profile in patients (including those with SAH), these data strongly support further studies to validate these findings in preclinical and clinical studies and to elucidate the mechanisms by which these agents might be acting.
Combining Multiband Imaging, Photogrammetric Techniques, and FOSS GIS for Affordable Degradation Mapping of Stone Monuments
Authors: Adamopoulos Efstathios , Rinaudo Fulvio
Published: July 2021

The detailed documentation of degradation constitutes a fundamental step for weathering diagnosis and, consequently, for successful planning and implementation of conservation measures for stone heritage. Mapping the surface patterns of stone is a non-destructive procedure critical for the qualitative and quantitative rating of the preservation state. Furthermore, mapping is employed for the annotation of weathering categories and the calculation of damage indexes. However, it is often a time-consuming task, which is conducted manually. Thus, practical methods need to be developed to automatize degradation mapping without significantly increasing the diagnostic process’s cost for conservation specialists. This work aims to develop and evaluate a methodology based on affordable close-range sensing techniques, image processing, and free and open source software for the spatial description, annotation, qualitative analysis, and rating of stone weathering-induced damage. Low-cost cameras were used to record images in the visible, near-infrared, and thermal-infrared spectra. The application of photogrammetric techniques allowed for the generation of the necessary background, that was elaborated to extract thematic information. Digital image processing of the spatially and radiometrically corrected images and image mosaics enabled the straightforward transition to a spatial information environment simplifying the development of degradation maps. The digital thematic maps facilitated the rating of stone damage and the extraction of useful statistical data.
Synoptic Diagnostics of Myeloproliferative Neoplasms: Morphology and Molecular Genetics
Authors: Nann Dominik , Fend Falko
Published: July 2021

The diagnosis of a myeloid neoplasm relies on a combination of clinical, morphological, immunophenotypic and genetic features, and an integrated, multimodality approach is needed for precise classification. The basic diagnostics of myeloid neoplasms still rely on cell counts and morphology of peripheral blood and bone marrow aspirate, flow cytometry, cytogenetics and bone marrow trephine biopsy, but particularly in the setting of Ph− myeloproliferative neoplasms (MPN), the trephine biopsy has a crucial role. Nowadays, molecular studies are of great importance in confirming or refining a diagnosis and providing prognostic information. All myeloid neoplasms of chronic evolution included in this review, nowadays feature the presence or absence of specific genetic markers in their diagnostic criteria according to the current WHO classification, underlining the importance of molecular studies. Crucial differential diagnoses of Ph− MPN are the category of myeloid/lymphoid neoplasms with eosinophilia and gene rearrangement of PDGFRA, PDGFRB or FGFR1, or with PCM1-JAK2, and myelodysplastic/myeloproliferative neoplasms (MDS/MPN). This review focuses on morphological, immunophenotypical and molecular features of BCR-ABL1-negative MPN and their differential diagnoses. Furthermore, areas of difficulties and open questions in their classification are addressed, and the persistent role of morphology in the area of molecular medicine is discussed.
CK2α/CSNK2A1 Induces Resistance to Doxorubicin through SIRT6-Mediated Activation of the DNA Damage Repair Pathway
Authors: Hussein Usama Khamis , Ahmed Asmaa Gamal , Song Yiping , Kim Kyoung Min , Moon Young Jae , Ahn Ae-Ri , Park Ho Sung , Ahn Su Jin , Park See-Hyoung , Kim Jung Ryul , Jang Kyu Yun
Published: July 2021

CK2α/CSNK2A1 is involved in cancer progression by phosphorylating various signaling molecules. Considering the role of CSNK2A1 in cancer progression and the phosphorylation of SIRT6 and the role of SIRT6 in chemoresistance through the DNA damage repair pathway, CSNK2A1 and SIRT6 might be involved in resistance to conventional anti-cancer therapies. We evaluated the expression of CSNK2A1 and phosphorylated SIRT6 in the 37 osteosarcoma patients and investigated the effects of CSNK2A1 and the phosphorylation of SIRT6 on Ser338 on resistance to the anti-cancer effects of doxorubicin. Higher expression of CSNK2A1 and phosphorylated SIRT6 was associated with shorter survival in osteosarcoma patients. U2OS and KHOS/NP osteosarcoma cells with induced overexpression of CSNK2A1 were resistant to the cytotoxic effects of doxorubicin, and the knock-down of CSNK2A1 potentiated the cytotoxic effects of doxorubicin. CSNK2A1 overexpression-mediated resistance to doxorubicin was associated with SIRT6 phosphorylation and the induction of the DNA damage repair pathway molecules. CSNK2A1- and SIRT6-mediated resistance to doxorubicin in vivo was attenuated via mutation of SIRT6 at the Ser338 phosphorylation site. Emodin, a CSNK2A1 inhibitor, potentiated the cytotoxic effects of doxorubicin in osteosarcoma cells. This study suggests that blocking the CSNK2A1-SIRT6-DNA damage repair pathway might be a new therapeutic stratagem for osteosarcomas.
WWOX and Its Binding Proteins in Neurodegeneration
Authors: Hsu Che-Yu , Lee Kuan-Ting , Sun Tzu-Yu , Sze Chun-I. , Huang Shenq-Shyang , Hsu Li-Jin , Chang Nan-Shan
Published: July 2021

WW domain-containing oxidoreductase (WWOX) is known as one of the risk factors for Alzheimer’s disease (AD), a neurodegenerative disease. WWOX binds Tau via its C-terminal SDR domain and interacts with Tau phosphorylating enzymes ERK, JNK, and GSK-3β, and thereby limits AD progression. Loss of WWOX in newborns leads to severe neural diseases and early death. Gradual loss of WWOX protein in the hippocampus and cortex starting from middle age may slowly induce aggregation of a protein cascade that ultimately causes accumulation of extracellular amyloid beta plaques and intracellular tau tangles, along with reduction in inhibitory GABAergic interneurons, in AD patients over 70 years old. Age-related increases in pS14-WWOX accumulation in the brain promotes neuronal degeneration. Suppression of Ser14 phosphorylation by a small peptide Zfra leads to enhanced protein degradation, reduction in NF-κB-mediated inflammation, and restoration of memory loss in triple transgenic mice for AD. Intriguingly, tumor suppressors p53 and WWOX may counteract each other in vivo, which leads to upregulation of AD-related protein aggregation in the brain and lung. WWOX has numerous binding proteins. We reported that the stronger the binding between WWOX and its partners, the better the suppression of cancer growth and reduction in inflammation. In this regard, the stronger complex formation between WWOX and partners may provide a better blockade of AD progression. In this review, we describe whether and how WWOX and partner proteins control inflammatory response and protein aggregation and thereby limit AD progression.
Recent Achievements in Electrochemical and Surface Plasmon Resonance Aptasensors for Mycotoxins Detection
Authors: Evtugyn Gennady , Porfireva Anna , Kulikova Tatjana , Hianik Tibor
Published: July 2021

Mycotoxins are secondary metabolites of fungi that contaminate agriculture products. Their release in the environment can cause severe damage to human health. Aptasensors are compact analytical devices that are intended for the fast and reliable detection of various species able to specifically interact with aptamers attached to the transducer surface. In this review, assembly of electrochemical and surface plasmon resonance (SPR) aptasensors are considered with emphasis on the mechanism of signal generation. Moreover, the properties of mycotoxins and the aptamers selected for their recognition are briefly considered. The analytical performance of biosensors developed within last three years makes it possible to determine mycotoxin residues in water and agriculture/food products on the levels below their maximal admissible concentrations. Requirements for the development of sample treatment and future trends in aptasensors are also discussed.
Electrochemical Immunosensors for Quantification of Procalcitonin: Progress and Prospects
Authors: Nellaiappan Subramanian , Mandali Pavan Kumar , Prabakaran Amrish , Krishnan Uma Maheswari
Published: July 2021

Human procalcitonin (PCT) is a peptide precursor of the calcium-regulating hormone calcitonin. Traditionally, PCT has been used as a biomarker for severe bacterial infections and sepsis. It has also been recently identified as a potential marker for COVID-19. Normally, serum PCT is intracellularly cleaved to calcitonin, which lowers the levels of PCT (<0.01 ng/mL). In severe infectious diseases and sepsis, serum PCT levels increase above 100 ng/mL in response to pro-inflammatory stimulation. Development of sensors for specific quantification of PCT has resulted in considerable improvement in the sensitivity, linear range and rapid response. Among the various sensing strategies, electrochemical platforms have been extensively investigated owing to their cost-effectiveness, ease of fabrication and portability. Sandwich-type electrochemical immunoassays based on the specific antigen–antibody interactions with an electrochemical transducer and use of nanointerfaces has augmented the electrochemical response of the sensors towards PCT. Identification of a superior combination of electrode material and nanointerface, and translation of the sensing platform into flexible and disposable substrates are under active investigation towards development of a point-of-care device for PCT detection. This review provides an overview of the existing detection strategies and limitations of PCT electrochemical immunosensors, and the emerging directions to address these lacunae.
Gestation-Based Viability–Difficult Decisions with Far-Reaching Consequences
Authors: Thomas Sumesh , Asztalos Elizabeth
Published: July 2021

Most clinicians rely on outcome data based on completed weeks of gestational of fetal maturity for antenatal and postnatal counseling, especially for preterm infants born at the margins of viability. Contemporary estimation of gestational maturity, based on ultrasounds, relies on the use of first-trimester scans, which offer an accuracy of ±3–7 days, and depend on the timing of the scans and the measurements used in the calculations. Most published literature on the outcomes of babies born prematurely have reported on short- and long-term outcomes based on completed gestational weeks of fetal maturity at birth. These outcome data change significantly from one week to the next, especially around the margin of gestational viability. With a change in approach solely from decisions based on survival, to disability-free survival and long-term functional outcomes, the complexity of the parental and care provider’s decision-making in the perinatal and postnatal period for babies born at less than 25 weeks gestation remains challenging. While sustaining life following birth at the margins of viability remains our priority—identifying and mitigating risks associated with extremely preterm birth begins in the perinatal period. The challenge of supporting the normal maturation of these babies postnatally has far-reaching consequences and depends on our ability to sustain life while optimizing growth, nutrition, and the repair of organs compromised by the consequences of preterm birth. This article aims to explore the ethical and medical complexities of contemporary decision-making in the perinatal and postnatal periods. We identify gaps in our current knowledge of this topic and suggest areas for future research, while offering a perspective for future collaborative decision-making and care for babies born at the margins of viability.
Treatment with Methylphenidate Improves Affective but Not Cognitive Empathy in Youths with Attention-Deficit/Hyperactivity Disorder
Authors: Fantozzi Pamela , Muratori Pietro , Caponi Maria Celeste , Levantini Valentina , Nardoni Cristina , Pfanner Chiara , Ricci Federica , Sesso Gianluca , Tacchi Annalisa , Milone Annarita , Masi Gabriele
Published: July 2021

Background: Beside the core symptoms, patients with attention-deficit/hyperactivity disorder (ADHD) frequently show relevant difficulty in developing relationships with peers. Although ADHD symptoms may account for social impairment, deficits in cognitive and/or affective empathy have also been involved. Our aim was to investigate the effect of methylphenidate (MPH) treatment on affective and cognitive empathy. Methods: Sixty-one drug-naïve youths with ADHD (age range 6 to 17 years, mean 10.3 ± 2.8 years, 51 males) naturalistically treated with MPH monotherapy were followed up for 6 months for ADHD symptoms and empathy, measured with the Basic Empathy Scale. Results: After being treated with MPH, the patients showed a significant improvement in affective and cognitive empathy scores. Linear regression models showed that changes in inattention symptoms predicted changes in affective but not in cognitive empathy, while changes in the hyperactivity/impulsivity symptoms did not predict changes in affective or cognitive empathy. Conclusions: Our study provides a further contribution for a better understanding of the possible effects of the MPH on youth’s characteristics.
Deactivation of SARS-CoV-2 via Shielding of Spike Glycoprotein Using Carbon Quantum Dots: Bioinformatic Perspective
Authors: Ramezani Zahra , Dayer Mohammad , Noorizadeh Siamak , Thompson Michael
Published: July 2021

The interaction of the spike (S) glycoprotein of SARS-CoV-2 with angiotensin-converting enzyme 2 (ACE2) correlates with increased virus transmissibility and disease severity in humans. Two strategies may be considered for preventive or treatment purposes: the blockage of the ACE2 receptors or the shielding of receptor-binding domains (RBD) in the Sprotein of COVID-19, as well as the S2 cleavage site that is used by the furin enzyme of the host cells in the late phase of virus activation. Herein, the interaction of carbon quantum dots (CQDs) with the Sprotein of SARS-CoV-2 was investigated using molecular docking and molecular dynamics. CQD molecules were optimized by the HF/3-21G level of theory; the probable interactions between the CQDs with Sprotein were studied by blind docking mode, considering the Sprotein as the receptor and CQDs as ligands. Ethanol, folic acid, Favipiravir, two kinds of functionalized triangular hexagonal graphene, and four kinds of functionalized CQDs were studied on a comparative basis. The results show that OH and amine-functionalized CQDs tend to interact with three branches of Sprotein, especially RBD. The fact that they can block the S2 cleavage site leads to their potential use as a therapeutic agent.
Minimum Round Card-Based Cryptographic Protocols Using Private Operations
Authors: Ono Hibiki , Manabe Yoshifumi
Published: July 2021

This paper shows new card-based cryptographic protocols with the minimum number of rounds, using private operations under the semi-honest model. Physical cards are used in card-based cryptographic protocols instead of computers to achieve secure multiparty computation. Operations that a player executes in a place where the other players cannot see are called private operations. Using three private operations—private random bisection cuts, private reverse cuts, and private reveals—the calculations of two variable Boolean functions and copy operations were realized with the minimum number of cards. Though the number of cards has been discussed, the efficiency of these protocols has not been discussed. This paper defines the number of rounds to evaluate the efficiency of the protocols, using private operations. Most of the meaningful calculations using private operations need at least two rounds. This paper presents a new two-round committed-input, committed-output logical XOR protocol, using four cards. Then, we show new two-round committed-input, committed-output logical AND and copy protocols, using six cards. Even if private reveal operations are not used, logical XOR, logical AND, and copy operations can be executed with the minimum number of rounds. Protocols for general n-variable Boolean functions and protocols that preserve an input are also shown. Lastly, protocols with asymmetric cards are shown.
Pressure-Tuned Superconducting Dome in Chemically-Substituted κ-(BEDT-TTF)2Cu2(CN)3
Authors: Saito Yohei , Löhle Anja , Kawamoto Atsushi , Pustogow Andrej , Dressel Martin
Published: July 2021

The quantum spin liquid candidate κ-(BEDT-TTF)2Cu2(CN)3 has been established as the prime example of a genuine Mott insulator that can be tuned across the first-order insulator–metal transition either by chemical substitution or by physical pressure. Here, we explore the superconducting state that occurs at low temperatures, when both methods are combined, i.e., when κ-[(BEDT-TTF)1−x(BEDT-STF)x]2Cu2(CN)3 is pressurized. We discovered superconductivity for partial BEDT-STF substitution with x = 0.10–0.12 even at ambient pressure, i.e., a superconducting state is realized in the range between a metal and a Mott insulator without magnetic order. Furthermore, we observed the formation of a superconducting dome by pressurizing the substituted crystals; we assigned this novel behavior to disorder emanating from chemical tuning.
Endothelial, Immunothrombotic, and Inflammatory Biomarkers in the Risk of Mortality in Critically Ill COVID-19 Patients: The Role of Dexamethasone
Authors: Keskinidou Chrysi , Vassiliou Alice G. , Zacharis Alexandros , Jahaj Edison , Gallos Parisis , Dimopoulou Ioanna , Orfanos Stylianos E. , Kotanidou Anastasia
Published: July 2021

Endothelial dysfunction, coagulation and inflammation biomarkers are increasingly emerging as prognostic markers of poor outcomes and mortality in severe and critical COVID-19. However, the effect of dexamethasone has not been investigated on these biomarkers. Hence, we studied potential prognostic biomarkers of mortality in critically ill COVID-19 patients who had either received or not dexamethasone. Biomarker serum levels were measured on intensive care unit (ICU) admission (within 24 h) in 37 dexamethasone-free and 29 COVID-19 patients who had received the first dose (6 mg) of dexamethasone. Receiver operating characteristic (ROC) curves were generated to assess their value in ICU mortality prediction, while Kaplan–Meier analysis was used to explore associations between biomarkers and survival. In the dexamethasone-free COVID-19 ICU patients, non-survivors had considerably higher levels of various endothelial, immunothrombotic and inflammatory biomarkers. In the cohort who had received one dexamethasone dose, non-survivors had higher ICU admission levels of only soluble (s) vascular cell adhesion molecule-1 (VCAM-1), soluble urokinase-type plasminogen activator receptor (suPAR) and presepsin. As determined from the generated ROC curves, sVCAM-1, suPAR and presepsin could still be reliable prognostic ICU mortality biomarkers, following dexamethasone administration (0.7 < AUC < 0.9). Moreover, the Kaplan–Meier survival analysis showed that patients with higher than the median values for sVCAM-1 or suPAR exhibited a greater mortality risk than patients with lower values (Log-Rank test, p < 0.01). In our single-center study, sVCAM-1, suPAR and presepsin appear to be valuable prognostic biomarkers in assessing ICU mortality risk in COVID-19 patients, even following dexamethasone administration.
Prediction of In-Hospital Cardiac Arrest Using Shallow and Deep Learning
Authors: Chae Minsu , Han Sangwook , Gil Hyowook , Cho Namjun , Lee Hwamin
Published: July 2021

Sudden cardiac arrest can leave serious brain damage or lead to death, so it is very important to predict before a cardiac arrest occurs. However, early warning score systems including the National Early Warning Score, are associated with low sensitivity and false positives. We applied shallow and deep learning to predict cardiac arrest to overcome these limitations. We evaluated the performance of the Synthetic Minority Oversampling Technique Ratio. We evaluated the performance using a Decision Tree, a Random Forest, Logistic Regression, Long Short-Term Memory model, Gated Recurrent Unit model, and LSTM–GRU hybrid models. Our proposed Logistic Regression demonstrated a higher positive predictive value and sensitivity than traditional early warning systems.
Optimal Insertion Depth for Nasal Mid-Turbinate and Nasopharyngeal Swabs
Authors: Callesen Rasmus Eið , Kiel Cecilie Mullerup , Hovgaard Lisette Hvid , Jakobsen Kathrine Kronberg , Papesch Michael , von Buchwald Christian , Todsen Tobias
Published: July 2021

Millions of people are tested for COVID-19 daily during the pandemic, and a lack of evidence to guide optimal nasal swab testing can increase the risk of false-negative test results. This study aimed to determine the optimal insertion depth for nasal mid-turbinate and nasopharyngeal swabs. The measurements were made with a flexible endoscope during the collection of clinical specimens with a nasopharyngeal swab at a public COVID-19 test center in Copenhagen, Denmark. Participants were volunteer adults undergoing a nasopharyngeal SARS-CoV-2 rapid antigen test. All 109 participants (100%) completed the endoscopic measurements; 52 (48%) women; 103 (94%) white; mean age 34.39 (SD, 13.2) years; and mean height 176.7 (SD, 9.29) cm. The mean swab length to the posterior nasopharyngeal wall was 9.40 (SD, 0.64) cm. The mean endoscopic distance to the anterior and posterior end of the inferior turbinate was 1.95 (SD, 0.61) cm and 6.39 (SD, 0.62) cm, respectively. The mean depth to nasal mid-turbinate was calculated as 4.17 (SD, 0.48) cm. The optimal depths of insertion for nasal mid-turbinate swabs are underestimated in current guidelines compared with our findings. This study provides clinical evidence to guide the performance of anatomically correct nasal and nasopharyngeal swab specimen collection for virus testing.
Sjogren’s Syndrome Presenting with Solely Cutaneous Features
Authors: Centala Sneha , Park Joyce H. , Girnita Diana
Published: July 2021

Sjogren’s syndrome is classically characterized by symptoms of keratoconjunctivitis sicca and xerostomia, secondary to lymphocytic infiltration of the salivary and lacrimal glands. Cutaneous findings of this disease are infrequently discussed and thus rarely considered among patients without the typical symptomatology. However, these patients can develop xerosis, alopecia, vitiligo, papular or nodular lesions, or cutaneous vasculitis. A 56-year-old Asian female presented with intermittent cutaneous erythematous lesions of her bilateral pinna and preauricular areas. Despite initial symptom presentation causing concern for tumid lupus versus cutaneous T cell lymphoma versus relapsing polychondritis, extensive serologic and histopathologic workup eventually indicated a likely diagnosis of Sjogren’s syndrome. This case brings to light that Sjogren’s syndrome is truly a multi-systemic disease and can present with primarily extra glandular cutaneous symptoms. When approaching the workup of a new patient, it is absolutely vital to maintain a broad differential and keep in mind that overlap syndromes among multiple autoimmune diseases do exist as well.
Maternal Bochdalek Hernia during Pregnancy: A Systematic Review of Case Reports
Authors: Choi Jin-Young , Yang Song-Soo , Lee Jong-Hwa , Roh Hyun-Jin , Ahn Jun-Woo , Kim Jeong-Sook , Lee Soo-Jeong , Lee Sang-Hun
Published: July 2021

Background: Since the first report of a diaphragmatic hernia from Ambroise Paré’s necropsy in 1610, the Bochdalek hernia (BH) of the congenital diaphragmatic hernia (CDH) has been the most common types with high morbidity and mortality in the neonatal period. Due to the nature of the disease, CDH associated with pregnancy is too infrequent to warrant reporting in the literature. Mortality of obstruction or strangulation is mostly due to failure to diagnose symptoms early. Data sources and study selection: A systematic literature search of maternal BH during pregnancy was conducted using the electronic databases (PubMed and EMBASE) from January 1941 to December 2020. Because of the rarity of the disease, this review included all primary studies, including case reports or case series that reported at least one case of maternal BH in pregnant. Searches, paper selection, and data extraction were conducted in duplicate. The analysis was performed narratively regardless of the control groups’ presence due to their rarity. Results: The search retrieved 3450 papers, 94 of which were deemed eligible and led to a total of 43 cases. Results of treatment showed 16 cases in delayed delivery after hernia surgery, 10 cases in simultaneous delivery with hernia surgery, 3 cases in non-surgical treatment, and 14 cases in hernia surgery after delivery. Of 16 cases with delayed delivery after hernia surgery, 13 (81%) cases had emergency surgery and three (19%) cases had surgery after expectant management. Meanwhile, 10 cases underwent simultaneous delivery with hernia surgery, 6 cases (60%) had emergent surgery, and 4 cases (40%) had delayed hernia surgery after expectant management. 3 cases underwent non-surgical treatment. In this review, the maternal death rate and fetal/neonatal loss rate from maternal BH was 5% (2/43) and 16% (7/43), respectively. The preterm birth rate has been reported in 35% (15/43) of maternal BH, resulting from maternal deaths in 13% (2/15) of cases and 6 fetal loss in 40% (6/15) of cases; 44% (19/43) of cases demonstrated signs of bowel obstruction, ischemia, or perforation of strangulated viscera in the operative field, resulting from maternal deaths in 11% (2/19) of cases and fetal-neonatal loss in 21% (4/19) of cases. Conclusion: Early diagnosis and surgical intervention are imperative, as a gangrenous or non-viable bowel resection significantly increases mortality. Therefore, multidisciplinary care should be required in maternal BH during pregnancies that undergo surgically repair, and individualized care allow for optimal results for the mother and fetus.
Broad Coverage Precoding for 3D Massive MIMO with Huge Uniform Planar Arrays
Authors: Lu An-An , Chen Yan , Gao Xiqi
Published: July 2021

In this paper, we propose a novel broad coverage precoder design for three-dimensional (3D) massive multi-input multi-output (MIMO) equipped with huge uniform planar arrays (UPAs). The desired two-dimensional (2D) angle power spectrum is assumed to be separable. We use the per-antenna constant power constraint and the semi-unitary constraint which are widely used in the literature. For normal broad coverage precoder design, the dimension of the optimization space is the product of the number of antennas at the base station (BS) and the number of transmit streams. With the proposed method, the design of the high-dimensional precoding matrices is reduced to that of a set of low-dimensional orthonormal vectors, and of a pair of low-dimensional vectors. The dimensions of the vectors in the set and the pair are the number of antennas per column and per row of the UPA, respectively. We then use optimization methods to generate the set of orthonormal vectors and the pair of vectors, respectively. Finally, simulation results show that the proposed broad coverage precoding matrices achieve nearly the same performance as the normal broad coverage precoder with much lower computational complexity.
Single-Particle Tracking Reveals Anti-Persistent Subdiffusion in Cell Extracts
Authors: Speckner Konstantin , Weiss Matthias
Published: July 2021

Single-particle tracking (SPT) has become a powerful tool to quantify transport phenomena in complex media with unprecedented detail. Based on the reconstruction of individual trajectories, a wealth of informative measures become available for each particle, allowing for a detailed comparison with theoretical predictions. While SPT has been used frequently to explore diffusive transport in artificial fluids and inside living cells, intermediate systems, i.e., biochemically active cell extracts, have been studied only sparsely. Extracts derived from the eggs of the clawfrog Xenopus laevis, for example, are known for their ability to support and mimic vital processes of cells, emphasizing the need to explore also the transport phenomena of nano-sized particles in such extracts. Here, we have performed extensive SPT on beads with 20 nm radius in native and chemically treated Xenopus extracts. By analyzing a variety of distinct measures, we show that these beads feature an anti-persistent subdiffusion that is consistent with fractional Brownian motion. Chemical treatments did not grossly alter this finding, suggesting that the high degree of macromolecular crowding in Xenopus extracts equips the fluid with a viscoelastic modulus, hence enforcing particles to perform random walks with a significant anti-persistent memory kernel.
Entropy, Information, and the Updating of Probabilities
Authors: Caticha Ariel
Published: July 2021

This paper is a review of a particular approach to the method of maximum entropy as a general framework for inference. The discussion emphasizes pragmatic elements in the derivation. An epistemic notion of information is defined in terms of its relation to the Bayesian beliefs of ideally rational agents. The method of updating from a prior to posterior probability distribution is designed through an eliminative induction process. The logarithmic relative entropy is singled out as a unique tool for updating (a) that is of universal applicability, (b) that recognizes the value of prior information, and (c) that recognizes the privileged role played by the notion of independence in science. The resulting framework—the ME method—can handle arbitrary priors and arbitrary constraints. It includes the MaxEnt and Bayes’ rules as special cases and, therefore, unifies entropic and Bayesian methods into a single general inference scheme. The ME method goes beyond the mere selection of a single posterior, and also addresses the question of how much less probable other distributions might be, which provides a direct bridge to the theories of fluctuations and large deviations.
The Influence of Income and Currency Changes on Tourist Inflow to Norwegian Campsites: The Case of Swedish and German Visitors
Authors: Opstad Leiv , Hammervold Randi , Idsø Johannes
Published: July 2021

There are few published articles on the demand for campsites, despite this being an important segment of the tourism industry. The purpose of this study was to gain further understanding of this topic. Using publicly available data over a period of 20 years, income and currency elasticity were estimated for German and Swedish camping tourists by using a natural logarithmic regression model with time series data. The results showed that both income and the exchange rate influenced the number of overnight stays, but the impact was rather small. The income elasticity for Swedish visitors was significant with a value of about 0.5, while it was zero and not significant for German camping tourists. Appreciation of the euro was associated with more visitors from Germany, but the estimated exchange rate elasticity was below 1.0 (and significant). A stronger Swedish currency relative to the Norwegian currency did not appear to have an effect. However, a stronger Swedish exchange rate, measured in euros, had a positive impact on Swedish camping visitors in Norway. The reason might be that more Swedish residents spend holidays abroad, and there is complementarity among the neighboring countries. Such calculations provide useful information for tourist industry planning.
Improved Pair-Wise Detections of Differential Quasi-Orthogonal Space-Time Modulation with Four Transmit Antennas
Authors: Kim Hojun , Shang Yulong , Kim Seunghyeon , Jung Taejin
Published: July 2021

In this paper, we propose new complex and real pair-wise detection for conventional differential space–time modulations based on quasi-orthogonal design with four transmit antennas for general QAM. Since the new complex and real pair-wise detections allow the independent joint ML detection of two complex and real symbol pairs, respectively, the decoding complexity is the same as or lower than conventional differential detections. Simulation results show that the proposed detections exhibit almost identical performance with an optimum maximum-likelihood receiver, as well as improved performance compared with conventional pair-wise detections, especially for higher modulation order.
Differential Evolution Based Machine Learning Scheme for Secure Cooperative Spectrum Sensing System
Authors: Gul Noor , Kim Su Min , Ahmed Saeed , Khan Muhammad Sajjad , Kim Junsu
Published: July 2021

The secondary users (SUs) in cognitive radio networks (CRNs) can obtain reliable spectrum sensing information of the primary user (PU) channel using cooperative spectrum sensing (CSS). Multiple SUs share their sensing observations in the CSS system to tackle fading and shadowing conditions. The presence of malicious users (MUs) may pose threats to the performance of CSS due to the reporting of falsified sensing data to the fusion center (FC). Different categories of MUs, such as always yes, always no, always opposite, and random opposite, are widely investigated by researchers. To this end, this paper proposes a hybrid boosted tree algorithm (HBTA)-based solution that combines the differential evolution (DE) and boosted tree algorithm (BTA) to mitigate the effects of MUs in the CSS systems, leading to reliable sensing results. An optimized threshold and coefficient vector, determined against the SUs employing DE, is utilized to train the BTA. The BTA is a robust ensembling machine learning (ML) technique gaining attention in spectrum sensing operations. To show the effectiveness of the proposed scheme, extensive simulations are performed at different levels of signal-to-noise-ratios (SNRs) and with different sensing samples, iteration levels, and population sizes. The simulation results show that more reliable spectrum decisions can be achieved compared to the individual utilization of DE and BTA schemes. Furthermore, the obtained results show the minimum sensing error to be exhibited by the proposed HBTA employing a DE-based solution to train the BTA. Additionally, the proposed scheme is compared with several other CSS schemes such as simple DE, simple BTA, maximum gain combination (MGC), particle swarm optimization (PSO), genetic algorithm (GA), and K-nearest neighbor (KNN) algorithm-based soft decision fusion (SDF) schemes to validate its effectiveness.
Biomethane Potential of Selected Organic Waste and Sewage Sludge at Different Temperature Regimes
Authors: Smetana German , Neczaj Ewa , Grosser Anna
Published: July 2021

Sewage sludge (SS) generation and its management still pose a problem in many countries. Anaerobic co-digestion (AcD) of SS with grease trap sludge (GTS) and organic fraction of municipal sewage waste (OFMSW), which are two easily biodegradable substrates, may improve biogas production and AcD kinetics. Algae biomass (AB) of the species Undaria pinnatifida can be the third co-digestion component that may also affect the AcD performance. The aim of the study was therefore to evaluate the performance of mesophilic and thermophilic SS batch AcD with OFMSW, GTS as well as AB through biochemical methane potential (BMP) assay in relation to cumulative specific biogas (YB) and methane yields (Ym). Three kinetic models were applied within the scope of the kinetic study. Results of the study showed that the mixture containing SS, GTS and AB brought the most noticeable improvements in Ym compared to other studied mixtures and in respect to standalone SS digestion, the improvement amounted to 88.37% at mesophilic temperature (260.83 ± 15.02 N mL CH4/g-VSadd and for standalone SS 138.47 ± 4.70 N mL CH4/g-VSadd) and 71.09%, respectively, at the thermophilic one (275.66 ± 4.11 N mL-CH4/g-VSadd and for SS standalone 161.13 ± 13.11 N mL-CH4/g-VSadd).
Torrefaction and Thermochemical Properties of Agriculture Residues
Authors: Akhtar Javaid , Imran Muhammad , Ali Arshid Mahmood , Nawaz Zeeshan , Muhammad Ayyaz , Butt Rehan Khalid , Jillani Maria Shahid , Naeem Hafiz Amir
Published: July 2021

In this study, the densification of three agriculture waste biomasses (corn cobs, cotton stalks, and sunflower) is investigated using the torrefaction technique. The samples were pyrolyzed under mild temperature conditions (200–320 °C) and at different residence times (10 min–60 min). The thermal properties of the obtained bio-char samples were analyzed via thermo-gravimetric analysis (TGA). Compositional analysis of the torrefied samples was also carried out to determine the presence of hemicellulose, cellulose, and lignin contents. According to the results of this study, optimum temperature conditions were found to be 260 °C–300 °C along with a residence time of 20 min–30 min. Based on the composition analysis, it was found that biochar contains more lignin and celluloses and lower hemicellulose contents than do the original samples. The removal of volatile hemicelluloses broke the interlocking of biomass building blocks, rendering biochar brittle, grindable, and less reactive. The results of this study would be helpful in bettering our understanding of the conversion of agricultural waste residues into valuable, solid biofuels for use in energy recovery schemes. The optimum temperature condition, residence time, and GCV for torrefied corn cobs were found to be 290 °C, 20 min, and 5444 kcal/kg, respectively. The optimum temperature condition, residence time, and GCV for torrefied cotton balls were found to be 270 °C, 30 min, and 4481 Kcal/kg, respectively. In the case of sunflower samples, the mass yield of the torrefied sample decreased from 85% to 71% by increasing the residence time from 10 min to 60 min, respectively.
Time-Dependent Climate Impact of Utilizing Residual Biomass for Biofuels—The Combined Influence of Modelling Choices and Climate Impact Metrics
Authors: Olofsson Johanna
Published: July 2021

Understanding the influence of method choices on results in life-cycle assessments is essential to draw informed conclusions. As the climate impact of bioenergy remains a debated topic, the focus of this study is how the chosen temporal framing influences a comparison of the climate impact of utilizing residual biomass for biofuel production to that of leaving the biomass to decay. In order to compare the biofuel scenario to its corresponding reference scenario where biomass is left to decay, a variety of analytical approaches were used: using time-aggregated and time-dependent life-cycle inventories and climate-impact assessment methods, assuming biogenic carbon to be climate neutral or not, using metrics for cumulative or instantaneous climate impact, and with different time horizons. Two cases of residual biofuel feedstocks were assessed: logging residues from Norway spruce forest, and straw from wheat cultivation. Consideration of the studied method choices appears to be especially relevant for forest residual biomass, as illustrated by the ranges of parity times for logging residues (25 to 95 years), and the results which vary with the chosen climate-impact metric, time-horizon, and approach for including biogenic carbon. Illustrating the time-dependence of results can, in general, provide a better understanding of the climate impact of utilizing residual biomass for biofuels.