bluuebunny/crossref_metadata_2025 huggingface dataset:
Sigma Factor Engineering in Actinoplanes sp. SE50/110: Expression of the Alternative Sigma Factor Gene ACSP50_0507 (σHAs) Enhances Acarbose Yield and Alters Cell Morphology
Authors: Schlüter Laura , Busche Tobias , Bondzio Laila , Hütten Andreas , Niehaus Karsten , Schneiker-Bekel Susanne , Pühler Alfred , Kalinowski Jörn
Published: June 2024

Sigma factors are transcriptional regulators that are part of complex regulatory networks for major cellular processes, as well as for growth phase-dependent regulation and stress response. Actinoplanes sp. SE50/110 is the natural producer of acarbose, an α-glucosidase inhibitor that is used in diabetes type 2 treatment. Acarbose biosynthesis is dependent on growth, making sigma factor engineering a promising tool for metabolic engineering. ACSP50_0507 is a homolog of the developmental and osmotic-stress-regulating Streptomyces coelicolor σHSc. Therefore, the protein encoded by ACSP50_0507 was named σHAs. Here, an Actinoplanes sp. SE50/110 expression strain for the alternative sigma factor gene ACSP50_0507 (sigHAs) achieved a two-fold increased acarbose yield with acarbose production extending into the stationary growth phase. Transcriptome sequencing revealed upregulation of acarbose biosynthesis genes during growth and at the late stationary growth phase. Genes that are transcriptionally activated by σHAs frequently code for secreted or membrane-associated proteins. This is also mirrored by the severely affected cell morphology, with hyperbranching, deformed and compartmentalized hyphae. The dehydrated cell morphology and upregulation of further genes point to a putative involvement in osmotic stress response, similar to its S. coelicolor homolog. The DNA-binding motif of σHAs was determined based on transcriptome sequencing data and shows high motif similarity to that of its homolog. The motif was confirmed by in vitro binding of recombinantly expressed σHAs to the upstream sequence of a strongly upregulated gene. Autoregulation of σHAs was observed, and binding to its own gene promoter region was also confirmed.
Bacillus amyloliquefaciens LM-1 Affects Multiple Cell Biological Processes in Magnaporthe oryzae to Suppress Rice Blast
Authors: Liang Meiling , Feng Aiqing , Wang Congying , Zhu Xiaoyuan , Su Jing , Xu Zihan , Yang Jianyuan , Wang Wenjuan , Chen Kailing , Chen Bing , Lin Xiaopeng , Feng Jinqi , Chen Shen
Published: June 2024

Magnaporthe oryzae, one of the most destructive rice pathogens, causes significant losses during the rice harvest every year. Bacillus amyloliquefaciens has been explored in many crops as a potential biocontrol agent. However, the mechanisms of B. amyloliquefaciens controled rice blast are not fully understood. Here, a biocontrol strain LM-1, isolated from a contaminated medium, was identified as B. amyloliquefaciens using morphological observation, physiological and biochemical tests, and 16S rDNA sequencing. LM-1 inhibited the growth and pathogenicity of M. oryzae and Bipolaris oryzae (Breda de Haan) Shoem. The mycelia of M. oryzae co-cultured with LM-1 were enlarged and broken by fluorescence microscopy using calcofluor white. LM-1 inhibited the mycelia of M. oryzae from producing conidia. Genes itu, srf, and fenB were detected in LM-1. Furthermore, the supernatant of LM-1 interfered with the appressorium formation of M. oryzae, blocked conidial cell death, and reduced autophagy degradation but did not affect the normal germination of rice seeds and seeding growth. Additionally, we observed hypersensitivity reactions, reactive oxygen species, and iron accumulation reduction in rice cells inoculated with supernatant. Our study reveals that LM-1 has a control effect on rice blast and affects cell wall integrity, sporulation, appressorium formation, cell death, and autophagy.
Whole-Genome Sequencing of Two Potentially Allelopathic Strains of Bacillus from the Roots of C. equisetifolia and Identification of Genes Related to Synthesis of Secondary Metabolites
Authors: Wang Ying , Chen Pan , Lin Qi , Zuo Linzhi , Li Lei
Published: June 2024

The coastal Casuarina equisetifolia is the most common tree species in Hainan’s coastal protection forests. Sequencing the genomes of its allelopathic endophytes can allow the protective effects of these bacteria to be effectively implemented in protected forests. The goal of this study was to sequence the whole genomes of the endophytes Bacillus amyloliquefaciens and Bacillus aryabhattai isolated from C. equisetifolia root tissues. The results showed that the genome sizes of B. amyloliquefaciens and B. aryabhattai were 3.854 Mb and 5.508 Mb, respectively. The two strains shared 2514 common gene families while having 1055 and 2406 distinct gene families, respectively. The two strains had 283 and 298 allelochemical synthesis-associated genes, respectively, 255 of which were shared by both strains and 28 and 43 of which were unique to each strain, respectively. The genes were putatively involved in 11 functional pathways, including secondary metabolite biosynthesis, terpene carbon skeleton biosynthesis, biosynthesis of ubiquinone and other terpene quinones, tropane/piperidine and piperidine alkaloids biosynthesis, and phenylpropanoid biosynthesis. NQO1 and entC are known to be involved in the biosynthesis of ubiquinone and other terpenoid quinones, and rfbC/rmlC, rfbA/rmlA/rffH, and rfbB/rmlB/rffG are involved in the biosynthesis of polyketide glycan units. Among the B. aryabhattai-specific allelochemical synthesis-related genes, STE24 is involved in terpene carbon skeleton production, atzF and gdhA in arginine biosynthesis, and TYR in isoquinoline alkaloid biosynthesis. B. amyloliquefaciens and B. aryabhattai share the genes aspB, yhdR, trpA, trpB, and GGPS, which are known to be involved in the synthesis of carotenoids, indole, momilactones, and other allelochemicals. Additionally, these bacteria are involved in allelochemical synthesis via routes such as polyketide sugar unit biosynthesis and isoquinoline alkaloid biosynthesis. This study sheds light on the genetic basis of allelopathy in Bacillus strains associated with C. equisetifolia, highlighting the possible use of these bacteria in sustainable agricultural strategies for weed management and crop protection.
The Function of Probiotics and Prebiotics on Canine Intestinal Health and Their Evaluation Criteria
Authors: Xia Junliang , Cui Yuling , Guo Yan , Liu Yuwen , Deng Baichuan , Han Sufang
Published: June 2024

Maintaining homeostasis within the intestinal microbiota is imperative for assessing the health status of hosts, and dysbiosis within the intestinal microbiota is closely associated with canine intestinal diseases. In recent decades, the modulation of canine intestinal health through probiotics and prebiotics has emerged as a prominent area of investigation. Evidence indicates that probiotics and prebiotics play pivotal roles in regulating intestinal health by modulating the intestinal microbiota, fortifying the epithelial barrier, and enhancing intestinal immunity. This review consolidates literature on using probiotics and prebiotics for regulating microbiota homeostasis in canines, thereby furnishing references for prospective studies and formulating evaluation criteria.
Evaluation of Functional Properties of Some Lactic Acid Bacteria Strains for Probiotic Applications in Apiculture
Authors: Urcan Adriana Cristina , Criste Adriana Dalila , Bobiș Otilia , Cornea-Cipcigan Mihaiela , Giurgiu Alexandru-Ioan , Dezmirean Daniel Severus
Published: June 2024

This study evaluates the suitability of three lactic acid bacteria (LAB) strains—Lactiplantibacillus plantarum, Lactobacillus acidophilus, and Apilactobacillus kunkeei—for use as probiotics in apiculture. Given the decline in bee populations due to pathogens and environmental stressors, sustainable alternatives to conventional treatments are necessary. This study aimed to assess the potential of these LAB strains in a probiotic formulation for bees through various in vitro tests, including co-culture interactions, biofilm formation, auto-aggregation, antioxidant activity, antimicrobial activity, antibiotic susceptibility, and resistance to high osmotic concentrations. This study aimed to assess both the individual effects of the strains and their combined effects, referred to as the LAB mix. Results indicated no mutual antagonistic activity among the LAB strains, demonstrating their compatibility with multi-strain probiotic formulations. The LAB strains showed significant survival rates under high osmotic stress and simulated gastrointestinal conditions. The LAB mix displayed enhanced biofilm formation, antioxidant activity, and antimicrobial efficacy against different bacterial strains. These findings suggest that a probiotic formulation containing these LAB strains could be used for a probiotic formulation, offering a promising approach to mitigating the negative effects of pathogens. Future research should focus on in vivo studies to validate the efficacy of these probiotic bacteria in improving bee health.
Metagenomic Investigation of the Short-Term Temporal and Spatial Dynamics of the Bacterial Microbiome and the Resistome Downstream of a Wastewater Treatment Plant in the Iskar River in Bulgaria
Authors: Donchev Deyan , Ivanov Ivan N. , Stoikov Ivan , Ivanova Monika
Published: June 2024

Waste Water Treatment Plants (WWTP) aim to reduce contamination in effluent water; however, studies indicate antimicrobial resistance genes (ARGs) persist post-treatment, potentially leading to their spread from human populated areas into the environment. This study evaluated the impact of a large WWTP serving 125,000 people on the Iskar River in Bulgaria, by characterizing the spatial and short-term temporal dynamics in bacterial community dynamics and resistance profiles of the surface water. Pairs of samples were collected biweekly on four dates from two different locations, one about 800 m after the WWTP effluents and the other 10 km downstream. Taxonomic classification revealed the dominance of Pseudomonodota and Bacteriodota, notably the genera Flavobacterium, Aquirufa, Acidovorax, Polynucleobacter, and Limnohabitans. The taxonomic structure corresponded with both lentic and lotic freshwater habitats, with Flavobacterium exhibiting a significant decrease over the study period. Principal Coordinate Analysis revealed statistically significant differences in bacterial community composition between samples collected on different dates. Differential abundance analysis identified notable enrichment of Polynucleobacter and Limnohabitans. There were shifts within the enriched or depleted bacterial taxa between early and late sampling dates. High relative abundance of the genes erm(B), erm(F), mph(E), msr(E) (macrolides); tet(C), tet(O), tet(W), tet(Q) and tet(X) (tetracyclines); sul1 and sul2 (sulphonamides); and cfxA3, cfxA6 (beta-lactams) were detected, with trends of increased presence in the latest sampling dates and in the location closer to the WWTP. Of note, genes conferring resistance to carbapenems blaOXA-58 and blaIMP-33-like were identified. Co-occurrence analysis of ARGs and mobile genetic elements on putative plasmids showed few instances, and the estimated human health risk score (0.19) according to MetaCompare2.0 was low. In total, 29 metagenome-assembled genomes were recovered, with only a few harbouring ARGs. This study enhances our understanding of freshwater microbial community dynamics and antibiotic resistance profiles, highlighting the need for continued ARGs monitoring.
Endophytic Microorganisms in Tomato Roots, Changes in the Structure and Function of the Community at Different Growing Stages
Authors: Wei Yufei , Chen Siyu , Zhou Xinyan , Ding Diancao , Song Jingjing , Yang Shangdong
Published: June 2024

This study analyzed flower bud differentiation and fruiting stages to investigate how the structure of the plant endophytic microbial community in the roots of tomatoes changes with plant senescence. Based on high-throughput sequencing technology, the diversity and relative abundance of endophytic microorganisms (bacteria and fungi) in tomato stems at different growth stages were analyzed. At the same time, based on LEfSe analysis, the differences in endophytic microorganisms in tomato stems at different growth stages were studied. Based on PICRUSt2 function prediction and FUNGuild, we predicted the functions of endophytic bacterial and fungal communities in tomato stems at different growth stages to explore potential microbial functional traits. The results demonstrated that not only different unique bacterial genera but also unique fungal genera could be found colonizing tomato roots at different growth stages. In tomato seedlings, flower bud differentiation, and fruiting stages, the functions of colonizing endophytes in tomato roots could primarily contribute to the promotion of plant growth, stress resistance, and improvement in nutrient cycling, respectively. These results also suggest that different functional endophytes colonize tomato roots at different growth stages.
Major, Trace and Rare Earth Elements Geochemistry of Bottom Sediments in the Retiro Baixo Reservoir after the B1 Tailings Dam Rupture, Paraopeba River (Brazil)
Authors: Sardinha Diego S , Pinto Mateus Sala , Menezes Paulo Henrique Bretanha Junker , Brucha Gunther , Silveira Jéssica Teixeira , Godoy Letícia Hirata , de Melo Deivid Arimatea Saldanha , Laureano Fernando Verassani
Published: June 2024

The rupture of an iron mining tailing dam in Brumadinho, Brazil, released around 10 million cubic meters of tailings, of which 1.6 Mm3 reached the Paraopeba River. In this work, a total of 30 samples from three bottom sediment cores were collected in the lower course of the Paraopeba River basin and analyzed for major, trace and rare earth elements by ICP-OES and ICP-MS. The sediments presented a range of compositions with different weathering histories, overall marked by depleted Ca2+, Na+ and K+ compared with the average UCC, PAAS and NASC and some advanced weathering trends. The samples presented a fractionation pattern characterized by a continuous depletion of light REEs from La to Sm and a regular decreased distribution of heavy REEs from Gd to Yb, and the Co/Th vs. La/Sc diagram indicates a predominant intermediate source. The upper samples presented the highest contents of REEs, probably due to the higher presence of iron and aluminum oxides and hydroxides, which can be related to more advanced weathering. The Al, Cu, Ni, V, Zn, Co, Mn, Ti, Fe and Si concentrations and the CF, EF and Igeo index values varied across the sediment core samples, demonstrating that there were long periods of geogenic or anthropogenic contributions.
Temperature Perturbation Infrared Spectroscopy of Minerals
Authors: Noneman Heidi F. , White Robert L.
Published: June 2024

A new method for variable temperature infrared spectroscopy studies of minerals is presented. A sample heating/cooling apparatus incorporating a modified button sample holder with thermoelectric temperature control is described. By employing different programs to heat and cool samples with temperatures varying in different ways, various aspects of mineral powders are investigated. Infrared spectroscopy methodologies for identifying sample structural changes as a function of temperature are described. The results obtained for a variety of minerals are provided as examples. The high precision and accuracy of this approach permit the detection of subtle crystallographic unit cell distortions as a function of temperature. A < 0.25% reduction in O-H stretching vibration band intensity associated with water desorption from a quartz sample is observed at 150 °C. By employing step temperature heating profiles, reversible and irreversible sample changes can be distinguished. Variable temperature infrared spectroscopy analyses demonstrate the utility of the technique for profiling sample dehydration processes and for elucidating interactions between mineral functionalities and absorbates as a function of temperature.
Precipitation of Precious Metals Concentrates from Post-Elution Solutions from Ion-Exchange Processes
Authors: Goc Karolina , Kluczka Joanna , Benke Grzegorz , Malarz Joanna , Pianowska Karolina , Leszczyńska-Sejda Katarzyna
Published: June 2024

Precious metals have long been considered as critical raw materials in many countries. There is a growing emphasis on recovering these metals from secondary sources such as automotive catalysts or WEEE (waste of electrical and electronic equipment). During the leaching process of these materials, solutions with low concentrations of precious metals are obtained, which necessitates the use of ion-exchange methods. Following sorption and elution, a post-elution solution called eluate is produced, containing precious metals and no impurities. This eluate must undergo further processing to obtain pure metals or its compounds. The objective of this study was to explore the feasibility of recovering precious metals from post-elution solutions through cementation, reduction, precipitation, or refining techniques. The analysis of the research results indicated that metallic zinc powder is the most effective cementing agent for platinum, palladium, rhodium, and gold. Metallic aluminum and copper powders can selectively cement gold and palladium, separating them from platinum and rhodium. Aqueous hydrazine hydrate solution is the best-reducing agent for precious metals, while an aqueous hydrogen peroxide solution can selectively reduce platinum and palladium, separating them from gold and rhodium.
Research on the Migration and Adsorption Mechanism Applied to Microplastics in Porous Media: A Review
Authors: Zeng Lin , Yuan Cong , Xiang Taoyu , Guan Xiangwei , Dai Li , Xu Dingliang , Yang Danhui , Li Long , Tian Chengcheng
Published: June 2024

In recent years, microplastics (MPs) have emerged as a significant environmental pollutant, garnering substantial attention for their migration and transformation behaviors in natural environments. MPs frequently infiltrate natural porous media such as soil, sediment, and rock through various pathways, posing potential threats to ecological systems and human health. Consequently, the migration and adsorption mechanisms applied to MPs in porous media have been extensively studied. This paper aims to elucidate the migration mechanisms of MPs in porous media and their influencing factors through a systematic review. The review encompasses the characteristics of MPs, the physical properties of porous media, and hydrodynamic factors. Additionally, the paper further clarifies the adsorption mechanisms of MPs in porous media to provide theoretical support for understanding their environmental behavior and fate. Furthermore, the current mainstream detection techniques for MPs are reviewed, with an analysis of the advantages, disadvantages, and applications of each technique. Finally, the paper identifies the limitations and shortcomings of current research and envisions future research directions.
The Effect of Photodynamic Therapy on Enterococcus spp. and Its Application in Dentistry: A Scoping Review
Authors: Rubilar-Huenchuman Mariaignacia , Ortega-Villanueva Camilo , González Iván A. , Palavecino Christian Erick
Published: June 2024

Enterococci spp. are Gram-positive bacteria that cause mild to severe infections, many associated with the oral cavity, such as periapical infections and healthcare-associated infections (HAIs). Many of these infections become serious diseases that are difficult to resolve, specifically when multidrug-resistant (MDR) strains cause them. In recent years, the number of MDR strains of Enterococcus spp. has increased significantly. This increased prevalence of MDR strains produces significant pressure to generate more antimicrobial therapies, but there is a decline in the production of new antibiotics, driving the development of complementary therapies, such as photodynamic therapy (PDT). PDT combines a photosensitizer agent (PS), light, and oxygen to cause photooxidative stress in bacterial cells. PDT can eradicate Enterococcus spp. contaminations, improve the classic cleaning processes, and eradicate the bacteria in dental pieces. PDT’s effectiveness can be improved with nanoparticles that function as carriers. Our work aims to describe the advances in PDT against Enterococcus spp. as a complement to antibiotic therapy, focusing on infections by Enterococcus faecium and Enterococcus faecalis, dental hygiene, and using nanoparticles to improve the antimicrobial effect. A systematic bibliographic search without a meta-analysis was conducted on various databases, using inclusion and exclusion criteria to identify the most relevant research. Of the 193 non-redundant articles found, 65 were selected for a systematic review, from which a summary table was created and a manual description was made. Photodynamic therapy for treating E. faecium and E. faecalis is a widely studied area, with promising results concerning bactericidal effectiveness and reductions in biofilm formation, particularly in regard to dental hygiene. Because most of the studies were conducted in vitro or ex vivo, the results indicated that there were not sufficient data to initiate clinical trials for safety and efficacy studies on humans.
Immunomodulatory and Anticancer Effects of Fridericia chica Extract-Loaded Nanocapsules in Myeloid Leukemia
Authors: de Freitas Gomes Alice , Batalha Adriane Dâmares de Souza Jorge , de Castro Alves Carlos Eduardo , Galvão de Azevedo Renata , Rodriguez Amado Jesus Rafael , Pereira de Souza Tatiane , Koolen Hector Henrique Ferreira , da Silva Felipe Moura Araújo , Chaves Francisco Celio Maia , Florentino Neto Serafim , Boechat Antônio Luiz , Soares Pontes Gemilson
Published: June 2024

Nanocapsules provide selective delivery and increase the bioavailability of bioactive compounds. In this study, we examined the anticancer and immunomodulatory potential of Fridericia chica (crajiru) extract encapsulated in nanocapsules targeting myeloid leukemias. Nanocapsules containing crajiru (nanocapsules-CRJ) were prepared via interfacial polymer deposition and solvent displacement. Size and polydispersity were measured by dynamic light scattering. Biological assays were performed on leukemia cell lines HL60 and K562 and on non-cancerous Vero cells and human PBMC. The anticancer activity was evaluated using cytotoxicity and clonogenic assays, while the immunomodulatory activity was evaluated by measuring the levels of pro- and anti-inflammatory cytokines in PBMC supernatants treated with concentrations of nanocapsules-CRJ. Nanocapsules-CRJ exhibited significant cytotoxic activity against HL60 and K562 cells at concentrations ranging from 0.75 to 50 μg/mL, with the greatest reductions in cell viability observed at 50 μg/mL (p < 0.001 for HL60; p < 0.01 for K562), while not affecting non-cancerous Vero cells and human PBMCs. At concentrations of 25 μg/mL and 50 μg/mL, nanocapsules-CRJ reduced the formation of HL60 and K562 colonies by more than 90% (p < 0.0001). Additionally, at a concentration of 12 μg/mL, nanocapsules-CRJ induced the production of the cytokines IL-6 (p = 0.0002), IL-10 (p = 0.0005), IL-12 (p = 0.001), and TNF-α (p = 0.005), indicating their immunomodulatory potential. These findings suggest that nanocapsules-CRJ hold promise as a potential therapeutic agent with both cytotoxic and immunomodulatory properties.
Interventions to Expand Community Pharmacists’ Scope of Practice
Authors: Ali Zaynah Zureen , Skouteris Helen , Pirotta Stephanie , Hussainy Safeera Yasmeen , Low Yi Ling , Mazza Danielle , Assifi Anisa Rojanapenkul
Published: June 2024

Background: The role of community pharmacists has evolved beyond the dispensing of medicines. The aim of this scoping review was to describe the interventions that expand the pharmacist’s scope of practice within a community pharmacy setting and assess their effectiveness. Methods: We performed a scoping review to identify randomised controlled trials (RCTs), published worldwide from 2013 to 2024, which focused on interventions designed to expand pharmacists’ scope of practice in the community. The review was undertaken in accordance with the Joanna Briggs Institute methodology for scoping reviews. To address the aim of this scoping review, the included RCTs were mapped to themes influenced by the Professional Practice Standards 2023 as developed by the Pharmaceutical Society of Australia: medication management, collaborative care and medication adherence. Results: Twelve studies demonstrated the potential to expand community pharmacists’ scope of practice. Two RCTs resulted in no effect of the intervention. One RCT (conducted in Italy) led to an actual change to community pharmacists’ scope of practice, with a statistically significant improvement in the proportion of patients with controlled asthma. Conclusions: On the whole, this scoping review synthesised the findings of peer-reviewed RCT studies that revealed expanding community pharmacists’ scope of practice may result in improved patient outcomes, a reduced burden for the healthcare system, and greater productivity.
Simulation Analysis of Mode Hopping Impacts on OFDR Sensing Performance
Authors: Wang Qirui , Lalam Nageswara , Zhao Kehao , Zhong Shuda , Zhang Guangyin , Wright Ruishu , Chen Kevin P.
Published: June 2024

This article examines the impacts of mode hopping on the sensing performance of optical frequency domain reflectometry (OFDR) and explores the potential for developing economical OFDR interrogators employing low-cost distributed feedback (DFB) lasers. By conducting numerical simulations, this study reveals that mode hopping has minimal effects on distance sensing measurements in free space due to the limited duration of beat interference signal at the incorrect frequency within the coherence length. Additionally, the simulations indicate that mode hopping only slightly affects the distributed strain sensing of OFDR, resulting in an error range of less than ±1µε when 100µε is applied to the sensing fiber. These findings highlight the potential of using low-cost DFB lasers with a 1-nm wavelength sweep range and a 1-MHz linewidth as tunable laser sources in OFDR while maintaining reliable and accurate sensing performance.
Genome-Wide Identification and Evolutionary and Expression Analyses of the Cyclin B Gene Family in Brassica napus
Authors: Li Mingyue , Zhang Minghao , Meng Boyu , Miao Likai , Fan Yonghai
Published: June 2024

Cyclin B (CYCB) is a regulatory subunit of cyclin-dependent kinase (CDK), the concentration of which fluctuates to regulate cell cycle progression. Extensive studies have been performed on cyclins in numerous species, yet the evolutionary relationships and biological functions of the CYCB family genes in Brassica napus remain unclear. In this study, we identified 299 CYCB genes in 11 B. napus accessions. Phylogenetic analysis suggests that CYCB genes could be divided into three subfamilies in angiosperms and that the CYCB3 subfamily members may be a newer group that evolved in eudicots. The expansion of BnaCYCB genes underwent segmental duplication and purifying selection in genomes, and a number of drought-responsive and light-responsive cis-elements were found in their promoter regions. Additionally, expression analysis revealed that BnaCYCBs were strongly expressed in the developing seed and silique pericarp, as confirmed by the obviously reduced seed size of the mutant cycb3;1 in Arabidopsis thaliana compared with Col-0. This study provides a comprehensive evolutionary analysis of CYCB genes as well as insight into the biological function of CYCB genes in B. napus.
Silicon Dioxide Nanoparticles-Based Amelioration of Cd Toxicity by Regulating Antioxidant Activity and Photosynthetic Parameters in a Line Developed from Wild Rice
Authors: Ashraf Humera , Ghouri Fozia , Liang Jiabin , Xia Weiwei , Zheng Zhiming , Shahid Muhammad Qasim , Fu Xuelin
Published: June 2024

An extremely hazardous heavy metal called cadmium (Cd) is frequently released into the soil, causing a considerable reduction in plant productivity and safety. In an effort to reduce the toxicity of Cd, silicon dioxide nanoparticles were chosen because of their capability to react with metallic substances and decrease their adsorption. This study examines the processes that underlie the stress caused by Cd and how SiO2NPs may be able to lessen it through modifying antioxidant defense, oxidative stress, and photosynthesis. A 100 μM concentration of Cd stress was applied to the hydroponically grown wild rice line, and 50 μM of silicon dioxide nanoparticles (SiO2NPs) was given. The study depicted that when 50 μM SiO2NPs was applied, there was a significant decrease in Cd uptake in both roots and shoots by 30.2% and 15.8% under 100 μM Cd stress, respectively. The results illustrated that Cd had a detrimental effect on carotenoid and chlorophyll levels and other growth-related traits. Additionally, it increased the levels of ROS in plants, which reduced the antioxidant capability by 18.8% (SOD), 39.2% (POD), 32.6% (CAT), and 25.01% (GR) in wild rice. Nevertheless, the addition of silicon dioxide nanoparticles reduced oxidative damage and the overall amount of Cd uptake, which lessened the toxicity caused by Cd. Reduced formation of reactive oxygen species (ROS), including MDA and H2O2, and an increased defense system of antioxidants in the plants provided evidence for this. Moreover, SiO2NPs enhanced the Cd resistance, upregulated the genes related to antioxidants and silicon, and reduced metal transporters’ expression levels.
Dual-Mode Ce-MOF Nanozymes for Rapid and Selective Detection of Hydrogen Sulfide in Aquatic Products
Authors: Cheng Qi , Du Xiaoyu , Fu Zuyao , Ding Zhaoyang , Xie Jing
Published: June 2024

Increasing concern over the safety of consumable products, particularly aquatic products, due to freshness issues, has become a pressing issue. Therefore, ensuring the quality and safety of aquatic products is paramount. To address this, a dual-mode colorimetric–fluorescence sensor utilizing Ce-MOF as a mimic peroxidase to detect H2S was developed. Ce-MOF was prepared by a conventional solvothermal synthesis method. Ce-MOF catalyzed the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB) by hydrogen peroxide (H2O2) to produce blue oxidized TMB (oxTMB). When dissolved, hydrogen sulfide (H2S) was present in the solution, and it inhibited the catalytic effect of Ce-MOF and caused the color of the solution to fade from blue to colorless. This change provided an intuitive indication for the detection of H2S. Through steady-state dynamic analysis, the working mechanism of this sensor was elucidated. The sensor exhibited pronounced color changes from blue to colorless, accompanied by a shift in fluorescence from none to light blue. Additionally, UV–vis absorption demonstrated a linear correlation with the H2S concentration, ranging from 200 to 2300 µM, with high sensitivity (limit of detection, LOD = 0.262 μM). Fluorescence intensity also showed a linear correlation, ranging from 16 to 320 µM, with high selectivity and sensitivity (LOD = 0.156 μM). These results underscore the sensor’s effectiveness in detecting H2S. Furthermore, the sensor enhanced the accuracy of H2S detection and fulfilled the requirements for assessing food freshness and safety.
A Multi-Objective Optimization of Neural Networks for Predicting the Physical Properties of Textile Polymer Composite Materials
Authors: Malashin Ivan , Tynchenko Vadim , Gantimurov Andrei , Nelyub Vladimir , Borodulin Aleksei
Published: June 2024

This paper explores the application of multi-objective optimization techniques, including MOPSO, NSGA II, and SPEA2, to optimize the hyperparameters of artificial neural networks (ANNs) and support vector machines (SVMs) for predicting the physical properties of textile polymer composite materials (TPCMs). The optimization process utilizes data on the physical characteristics of the constituent fibers and fabrics used to manufacture these composites. By employing optimization algorithms, we aim to enhance the predictive accuracy of the ANN and SVM models, thereby facilitating the design and development of high-performance textile polymer composites. The effectiveness of the proposed approach is demonstrated through comparative analyses and validation experiments, highlighting its potential for optimizing complex material systems.
Additive Manufacturing of Head Surrogates for Evaluation of Protection in Sports
Authors: Mantecón Ramiro , Valverde-Marcos Borja , Rubio Ignacio , Youssef George , Loya José Antonio , Díaz-Álvarez José , Miguélez María Henar
Published: June 2024

Head impacts are a major concern in contact sports and sports with high-speed mobility due to the prevalence of head trauma events and their dire consequences. Surrogates of human heads are required in laboratory testing to safely explore the efficacy of impact-mitigating mechanisms. This work proposes using polymer additive manufacturing technologies to obtain a substitute for the human skull to be filled with a silicone-based brain surrogate. This assembly was instrumentalized with an Inertial Measurement Unit. Its performance was compared to a standard Hybrid III head form in validation tests using commercial headgear. The tests involved impact velocities in a range centered around 5 m/s. The results show a reasonable homology between the head substitutes, with a disparity in the impact response within 20% between the proposed surrogate and the standard head form. The head surrogate herein developed can be easily adapted to other morphologies and will significantly decrease the cost of the laboratory testing of head protection equipment, all while ensuring the safety of the testing process.
Technical Support System for High Concurrent Power Trading Platforms Based on Microservice Load Balancing
Authors: Shao Ping , Huang Longda , Weng Liguo , Liu Ziheng
Published: June 2024

With the booming development of the electricity market, market factors such as electricity trading varieties are growing rapidly. The frequency of transactions has become increasingly real-time, and transaction clearing and settlement tasks have become more complex. The increasing demands for concurrent access and carrying capacity in trading systems have made it increasingly difficult for existing systems to support business. This article proposes a transaction support system for large-scale electricity trading market entities, which solves the problems of high concurrency access and massive access data calculation while ensuring system security through business isolation measures. The system uses microservices to treat various functional modules as independent service modules, thus making service segmentation and composition more flexible. By using read–write separation, caching mechanisms, and several data reliability assurance measures, data can be stored and accessed quickly and securely. The use of a three-layer load balancing module consisting of an OpenResty access entry layer, a gateway routing gateway layer, and a WebClient service inter-resource invocation layer can effectively improve the system’s ability to handle concurrent access.
Effects of Vegetation Cover Varying along the Hydrological Gradient on Microbial Community and N-Cycling Gene Abundance in a Plateau Lake Littoral Zone
Authors: Yuan Jing , Cao Jing , Liao Wanxue , Zhu Feng , Hou Zeying , Chu Zhaosheng
Published: June 2024

The lake littoral zone is periodically exposed to water due to water level fluctuations, driving the succession and distribution of littoral vegetation covers, which complexly affect nutrient biogeochemical transformation. However, the combined effects of water level fluctuations and other environmental factors on microbial characteristics and functions at the regional scale remain unclear. In this study, typical vegetation cover types along various water levels were chosen to investigate the effects of water level and vegetation cover on the microbial community and functional genes in the Lake Erhai littoral zone. The results showed that water level fluctuations influenced oxygen and nitrogen compound contents due to oxic–anoxic alternations and intensive material exchange. Meanwhile, vegetation cover affected the organic matter and total nitrogen content through plant residues and root exudation supplying exogenous carbon and nitrogen. Along the hydrological gradient, the high microbial diversity and abundant microbes related to nitrogen cycling were observed in interface sediments. It was attributed to the alternating aerobic–anaerobic environments, which filtered adaptable dominant phyla and genera. The abundances of amoA AOA, nirS, and amx were higher than those of the other genes and were strongly related to flooding days and water content. In conclusion, water level fluctuations and vegetation type jointly affect microbial community structure and nitrogen-related functional genes.
Properties of Ni-B/B Composite Coatings Produced by the Electroless Method under Semi-Technical Line Conditions
Authors: Cieślak Grzegorz , Gostomska Marta , Dąbrowski Adrian , Ciciszwili-Wyspiańska Tinatin , Skroban Katarzyna , Mazurek Anna , Wojda Edyta , Głowacki Michał , Rygier Tomasz , Gajewska-Midziałek Anna
Published: June 2024

Composite coatings have been successfully fabricated at the laboratory scale in many research centers around the world; however, it is still a major challenge to transfer the positive results of the work to the industrial scale. This paper presents the technology for the production of Ni-B and Ni-B/B composite coatings on a pilot experimental semi-technical line by chemical reduction. A process scheme for the fabrication of Ni-B layers and composite coatings with a nickel–boron matrix and a dispersive phase in the form of boron nanoparticles was developed. All stages of the fabrication process were described in detail. The dispersion phase of the boron particles was characterized, and the performance properties of the Ni-B and Ni-B/B composite coatings produced on a pilot electroplating line were studied. The structure and morphology of the Ni-B/B composite coatings were characterized for comparison with nickel–boron coatings. Their mechanical and tribological properties and adhesion to the substrate were studied. The influence of the dispersion phase of boron particles on the structure and functional properties of the composite coatings was evaluated. In order to improve the performance of the fabricated coatings, a heating process at 400 °C was carried out, and the performance of Ni-B and composite Ni-B/B coatings was studied after the heat treatment operation.
Micro-Groove Optimisation of High-Speed Inner Ring Micro-Grooved Pumping Seal for New Energy Electric Vehicles
Authors: Chen Hanqing , Yan Ruqi , Hong Xianzhi , Bao Xin , Ding Xuexing
Published: June 2024

Traditional oil seals are insufficient for the high-speed and bi-directional rotation of new energy electric vehicles. Therefore, we developed a Python program focusing on micro-groove pump seals and examined the unexplored non-contact oil–air biphasic internal end-face seals. Real gas effects were described using the virial and Lucas equations. We introduced an oil–air ratio to determine the equivalent density and viscosity of the two-phase fluid in the seal. Furthermore, we solved the compressible steady-state Reynolds equation using the finite difference method. Analysing the seal’s pumping mechanisms and the effects of operating parameters on sealing performance, we assessed 17 types of hydrodynamic grooves. The results demonstrate that inverse fir tree-like grooves perform well under typical sealing conditions. Under the conditions given in this study, the pumping rate of the optimal groove type compared to other groove types even reached 633.54%. In the oil–air biphasic state, the micro-groove pump seal exerts significant dynamic pressure on the sealing surface. Seal opening force increases with rotational velocity, oil–air ratio, and inlet pressure but decreases with temperature. The pumping rate first increases and then decreases with rotational velocity, increases with oil–air ratio and temperature, and then decreases with inlet pressure. Some special working points require consideration in sealing design. Our results provide insights into designing micro-grooved pumping seals for new energy electric vehicles.
From Criticism and Rejection to Sino-Western Communication: The Evolution of Zheng Guanying’s Understanding of the Spread of Christianity in China
Authors: Li Di
Published: June 2024

Zheng Guanying paid close attention to the issue of the spread of Christianity in China since his youth. Over a period of more than three decades, he produced five monographs specifically addressing Christianity, from unequivocally opposing the spread of Christianity in China in the mid-to-late 1870s, to advocating for the adoption of the preaching form of the Christian “gatherings every seven days” to disseminate the village covenants and sacred edicts among the Chinese people in the 1890s. He proposed that the Chinese people should hold the right to spread Christianity. In 1906, he advocated for the establishment of a “common religion” and proposed “one religion for all nations” to eliminate wars around the world. In his later years, he proposed the “Five great wishes” to integrate and govern the various religions of the world with Taoism as the core, attempting to reconstruct global order from the perspective of religious unity. He envisioned the establishment of a sacred Taoist monastery, the dissemination of religious concepts, and the cultivation of talents, which drew on the organizational structure and missionary methods of Christianity, reflecting the thinking and efforts of modern Chinese intellectuals to bridge the Chinese and Western civilizations, seek solutions for modern China, reconcile conflicts between China and the West, and pursue global unity.
Hg Pollution in Groundwater of Andean Region of Ecuador and Human Health Risk Assessment
Authors: Passarelli Irene , Mora-Silva Demmy , Jimenez-Gutierrez Mirian , Logroño-Naranjo Santiago , Hernández-Allauca Damaris , Valdez Rogelio Ureta , Avalos Peñafiel Victor Gabriel , Tierra Pérez Luis Patricio , Sanchez-Salazar Marcelo , Tobar Ruiz María Gabriela , Carrera-Silva Katherin , Straface Salvatore , Mestanza-Ramón Carlos
Published: June 2024

In Ecuador, illegal gold mining has led to significant environmental impacts, with the release of harmful elements such as mercury (Hg) into the environment. Mercury, due to its physical–chemical characteristics and the transport elements involved between different environmental matrices, can easily percolate through the soil and reach groundwater. The purpose of this study was to evaluate the mercury concentration levels in the Andean region in order to perform a human health risk assessment. For this purpose, 175 water samples were analyzed, of which 9.71% exceeded the maximum permissible limit (MPL) established for drinking water in accordance with Ecuadorian regulations. The risk analysis was conducted by applying two approaches: deterministic and probabilistic. The deterministic approach involves a specific analysis based on the calculation of the risk quotient (HQ) and risk index (HI) for both receptors (adults and children) and scenarios (residential and recreational) considered; the probabilistic approach is based on the use of stochastic simulation techniques. The results obtained from the two approaches show a discrepancy, with the deterministic analysis providing more conservative results; however, they coincide in showing higher risk for the child population; decision-makers could use these results to identify areas to be monitored and plan more detailed investigation plans.
Three-Degree-of-Freedom Cable-Driven Parallel Manipulator with Self-Sensing Nitinol Actuators
Authors: Durante Francesco , Raparelli Terenziano , Beomonte Zobel Pierluigi
Published: June 2024

This paper presents the design and analysis of a novel 3-degree-of-freedom (3-DOF) parallel manipulator equipped with self-sensing Ni-Ti (Nitinol) actuators. The manipulator’s architecture and mechanical design are elucidated, emphasizing the integration of Nitinol actuators. The self-sensing technique implemented in a previous work was extended to a 20 mm actuator length, and the actuator was used to design the 3-DOF manipulator. Kinematic analyses were conducted to evaluate the manipulator’s performance under various operating conditions. A dynamic model was implemented for the dynamic dimensioning of the actuators, which work synergistically with a bias spring. The manipulator was realized, and a control strategy was implemented. Experimental tests, although documenting some positioning accuracy issues, show the efficacy and potential applications of the proposed manipulator in robotics and automation systems, highlighting the advantages of self-sensing Nitinol actuators in small parallel manipulator designs.
Exploring Spatial Patterns of Tropical Peatland Subsidence in Selangor, Malaysia Using the APSIS-DInSAR Technique
Authors: de la Barreda-Bautista Betsabé , Ledger Martha J. , Sjögersten Sofie , Gee David , Sowter Andrew , Cole Beth , Page Susan E. , Large David J. , Evans Chris D. , Tansey Kevin J. , Evers Stephanie , Boyd Doreen S.
Published: June 2024

Tropical peatlands in Southeast Asia have experienced widespread subsidence due to forest clearance and drainage for agriculture, oil palm and pulp wood production, causing concerns about their function as a long-term carbon store. Peatland drainage leads to subsidence (lowering of peatland surface), an indicator of degraded peatlands, while stability/uplift indicates peatland accumulation and ecosystem health. We used the Advanced Pixel System using the Intermittent SBAS (ASPIS-DInSAR) technique with biophysical and geographical data to investigate the impact of peatland drainage and agriculture on spatial patterns of subsidence in Selangor, Malaysia. Results showed pronounced subsidence in areas subjected to drainage for agricultural and oil palm plantations, while stable areas were associated with intact forests. The most powerful predictors of subsidence rates were the distance from the drainage canal or peat boundary; however, other drivers such as soil properties and water table levels were also important. The maximum subsidence rate detected was lower than that documented by ground-based methods. Therefore, whilst the APSIS-DInSAR technique may underestimate absolute subsidence rates, it gives valuable information on the direction of motion and spatial variability of subsidence. The study confirms widespread and severe peatland degradation in Selangor, highlighting the value of DInSAR for identifying priority zones for restoration and emphasising the need for conservation and restoration efforts to preserve Selangor peatlands and prevent further environmental impacts.