bluuebunny/crossref_metadata_2025 huggingface dataset:
Agronomic and Environmental Implications of Substituting Pig Slurry for Synthetic Nitrogen in Mediterranean Wheat Systems
Authors: Mateo-Marín Noemí , Isla Ramón , Guillén Mónica , Quílez Dolores
Published: October 2020

Using slurries as fertilizers is a promising strategy for the reuse of nutrients and striving towards a circular economy. This study aims to assess the agronomic productivity and the environmental effects on Mediterranean sprinkler-irrigated wheat during three consecutive years of (1) the use of pig slurry (PS) as a substitute for a synthetic fertilizer (urea, U) and (2) the addition of a urease inhibitor (monocarbamide dihydrogen sulfate, MCDHS) to pig slurry (PSI). A nitrogen (N) target rate of 120 kg NH4+-N ha−1 as U, PS, or PSI (main factor) was applied at tillering, and it was supplemented with N application at stem elongation (secondary factor). Grain yield, nitrogen use efficiency indexes, and nitrous oxide emissions were not significantly affected by the N source, U, or PS; in contrast to grain protein that was affected by the N source (lower protein content in PS). The higher unaccounted N from soil balance in PS compared to U fertilization could be associated with higher ammonia volatilization, although additional studies should confirm this hypothesis. Our study suggests that, under the considered cropping conditions, PS does not affect productivity or N2O losses compared to U. The addition of MCDHS to pig slurry did not display agronomic or environmental benefits under the evaluated agro-environmental conditions.
Effects of Fermentation on Standardized Ileal Digestibility of Amino Acids and Apparent Metabolizable Energy in Rapeseed Meal Fed to Broiler Chickens
Authors: Wu Zhengke , Liu Jiao , Chen Jiang , Pirzado Shoaib Ahmed , Li Yang , Cai Huiyi , Liu Guohua
Published: October 2020

Rapeseed meal (RSM) is a common protein ingredient in animal diets, while the proportion of RSM in diets is limited because of its anti-nutritional factors. Fermentation based on mixed microbial strains appears to be a suitable approach to improve the nutritive value of rapeseed meal in animal feed. In this study, we evaluated the effects of fermentation on the apparent metabolizable energy (AME) values and standardized ileal digestibility (SID) of amino acids in RSM fed broilers. The AME and nitrogen-corrected apparent metabolizable energy (AMEn) values of RSM and fermented rapeseed meal (FRSM) were determined by the substitution method, with RSM and FRSM proportionally replacing the energy-yielding components of the basal diet by 30%. Results show that fermentation improved AME and AMEn of RSM from 7.44 to 8.51 MJ/kg and from 7.17 to 8.26 MJ/kg, respectively. In the second experiment, two experimental diets were formulated, with RSM and FRSM being the sole sources of amino acids. A nitrogen-free diet (NFD) was also formulated to determine endogenous amino acids losses (EAAL). Feeding on FRSM resulted in higher (p < 0.05) apparent ileal digestibility (AID) and SID of alanine, valine, isoleucine, leucine, tyrosine, lysine, arginine, and phenylalanine. No significant differences between RSM and FRSM were found for AID and SID of asparagine, histidine, threonine, serine, glutamine, praline, glycine, methionine, and cystine. FRSM had greater AMEn values and SID of amino acids compared to RSM, therefore, FRSM was nutritionally superior to RSM in broiler diets.
An Estimate of the Effects from Precision Livestock Farming on a Productivity Index at Farm Level. Some Evidences from a Dairy Farms’ Sample of Lombardy
Authors: Carillo Felicetta , Abeni Fabio
Published: October 2020

This paper aimed at verifying if and to what extent the use of information technologies for dairy farming positively affects productivity of farmed herd. To do this we estimated the effects of precision farming on a productivity index at herd level, utilizing individual farms data of about 500 livestock farms. Farms are specialized in bovine milk production and are localized in Lombardy, that is one of the most important areas of Italian dairy farming. Using a two-stage treatment regression model, to solve the selection bias due to both observed and un-observed individual heterogeneity in the technology adoption, the study found a positive relationship between adopter status and the proxy of herd productivity.
Coexistence of Three Divergent mtDNA Lineages in Northeast Asia Provides New Insights into Phylogeography of Goldfish (Carssius auratus)
Authors: Cheng Lei , Lu Cuiyun , Wang Le , Li Chao , Yu Xiaoli
Published: October 2020

Goldfish (Carassius aurautus), which is a middle size cyprinid, widely distribute throughout Eurasia. Phylogeographic studies using mtDNA markers have revealed several divergent lineages within goldfish. In this study, mtDNA variations were determined to elucidate the phylogeographical pattern and genetic structure of goldfish in Northeast Asia. A total of 1054 individuals from Amur river basin were analyzed, which including five newly collected populations and four previously reported populations. Three distinct mtDNA lineages were identified in those samples, two of which corresponded to two known lineages C2 and C6, respectively. The third lineage referred to as C7, following six known lineages of goldfish in mainland Eurasia. AMOVA results suggested that most of the genetic variations were among lineages, rather than among populations or twice samplings. We noted that the control region (CR) and cytochrome b (cytb) sequences of lineage C7 have been reported in previous studies, respectively. However, the evolutionary position and distribution pattern of this lineage was not discussed in the context of the species. Our results showed that “odd” CR and “hidden” cytb sequences from Central Asia represent the same mtDNA lineage of goldfish. The known samples of C7 lineage were collected from Central Asia (Eastern Kazakhstan and Western Mongolia) to East Asia (Northeast China and Far East Russia), which suggested that it had a wider distribution, rather than limit in Central Asia.
Multilevel Validation of Doppler Wind Lidar by the 325 m Meteorological Tower in the Planetary Boundary Layer of Beijing
Authors: Dai Lindong , Xin Jinyuan , Zuo Hongchao , Ma Yongxiang , Zhang Lei , Wu Xinrui , Ma Yongjing , Jia Danjie , Wu Fangkun
Published: October 2020

The high-frequency monitoring of three-dimensional wind fields is crucial in planetary boundary layer meteorology. Doppler wind lidar and meteorological towers are the most important instruments for site observations of three-dimensional wind fields. This study systematically investigated and compared the performances of three wind measurement instruments: A Doppler wind lidar (Windcube 100s), cup anemometer/wind vane and sonic wind anemometer mounted on the 325 m meteorological tower in the polluted urban city of Beijing. The horizontal wind speed measurements of the Doppler wind lidar closely matched those of the cup anemometer and the sonic wind anemometer with high coefficients of determination (R2: 0.79–0.96 and 0.90–0.97, respectively). Moreover, the results also showed good agreement between the three measurements of the prevailing horizontal wind direction. Conversely, there were weak correlations between the vertical wind speeds of the Doppler wind lidar and sonic wind anemometer with low coefficients of determination (R2: 0.30–0.46). With increasing temporal scale, the consistency in the vertical wind increased. In addition, the Doppler wind lidar seemed to correlate better with the sonic wind anemometer at heights exceeding 300 m (R2: 0.48–0.77). Note that there was a remarkable difference between the Doppler wind lidar and 325 m meteorological tower observations as the aerosol concentrations changed rapidly. Different wind measurement instruments have unique advantages and are thus irreplaceable. The Doppler wind lidar is better at measuring larger turbulent eddies.
Insight into Construction of Tikhonov-Type Regularization for Atmospheric Retrievals
Authors: Xu Jian , Rao Lanlan , Schreier Franz , Efremenko Dmitry S. , Doicu Adrian , Trautmann Thomas
Published: October 2020

In atmospheric science we are confronted with inverse problems arising in applications associated with retrievals of geophysical parameters. A nonlinear mapping from geophysical quantities (e.g., atmospheric properties) to spectral measurements can be represented by a forward model. An inversion often suffers from the lack of stability and its stabilization introduced by proper approaches, however, can be treated with sufficient generality. In principle, regularization can enforce uniqueness of the solution when additional information is incorporated into the inversion process. In this paper, we analyze different forms of the regularization matrix L in the framework of Tikhonov regularization: the identity matrix L0, discrete approximations of the first and second order derivative operators L1 and L2, respectively, and the Cholesky factor of the a priori profile covariance matrix LC. Each form of L has its intrinsic pro/cons and thus may lead to different performance of inverse algorithms. An extensive comparison of different matrices is conducted with two applications using synthetic data from airborne and satellite sensors: retrieving atmospheric temperature profiles from microwave spectral measurements, and deriving aerosol properties from near infrared spectral measurements. The regularized solution obtained with L0 possesses a reasonable magnitude, but its smoothness is not always assured. The retrieval using L1 and L2 produces a solution in favor of the smoothness, and the impact of the a priori knowledge is less critical on the retrieval using L1. The retrieval performance of LC is affected by the accuracy of the a priori knowledge.
Detection and Classification of Immature Leukocytes for Diagnosis of Acute Myeloid Leukemia Using Random Forest Algorithm
Authors: Dasariraju Satvik , Huo Marc , McCalla Serena
Published: October 2020

Acute myeloid leukemia (AML) is a fatal blood cancer that progresses rapidly and hinders the function of blood cells and the immune system. The current AML diagnostic method, a manual examination of the peripheral blood smear, is time consuming, labor intensive, and suffers from considerable inter-observer variation. Herein, a machine learning model to detect and classify immature leukocytes for efficient diagnosis of AML is presented. Images of leukocytes in AML patients and healthy controls were obtained from a publicly available dataset in The Cancer Imaging Archive. Image format conversion, multi-Otsu thresholding, and morphological operations were used for segmentation of the nucleus and cytoplasm. From each image, 16 features were extracted, two of which are new nucleus color features proposed in this study. A random forest algorithm was trained for the detection and classification of immature leukocytes. The model achieved 92.99% accuracy for detection and 93.45% accuracy for classification of immature leukocytes into four types. Precision values for each class were above 65%, which is an improvement on the current state of art. Based on Gini importance, the nucleus to cytoplasm area ratio was a discriminative feature for both detection and classification, while the two proposed features were shown to be significant for classification. The proposed model can be used as a support tool for the diagnosis of AML, and the features calculated to be most important serve as a baseline for future research.
Menstrual Cycle Modulates Motor Learning and Memory Consolidation in Humans
Authors: Ikarashi Koyuki , Sato Daisuke , Iguchi Kaho , Baba Yasuhiro , Yamashiro Koya
Published: October 2020

Numerous studies have noted that sex and/or menstrual phase influences cognitive performance (in particular, declarative memory), but the effects on motor learning (ML) and procedural memory/consolidation remain unclear. In order to test the hypothesis that ML differs across menstrual cycle phases, initial ML, overlearning, consolidation, and final performance were assessed in women in the follicular, preovulation and luteal phases. Primary motor cortex (M1) oscillations were assessed neuro-physiologically, and premenstrual syndrome and interoceptive awareness scores were assessed psychologically. We found not only poorer performance gain through initial ML but also lower final performance after overlearning a day and a week later in the luteal group than in the ovulation group. This behavioral difference could be explained by particular premenstrual syndrome symptoms and associated failure of normal M1 excitability in the luteal group. In contrast, the offline effects, i.e., early and late consolidation, did not differ across menstrual cycle phases. These results provide information regarding the best time in which to start learning new sensorimotor skills to achieve expected gains.
Mast Cells in the Auditory Periphery of Rodents
Authors: Szczepek Agnieszka J. , Dudnik Tatyana , Karayay Betül , Sergeeva Valentina , Olze Heidi , Smorodchenko Alina
Published: October 2020

Mast cells (MCs) are densely granulated cells of myeloid origin and are a part of immune and neuroimmune systems. MCs have been detected in the endolymphatic sac of the inner ear and are suggested to regulate allergic hydrops. However, their existence in the cochlea has never been documented. In this work, we show that MCs are present in the cochleae of C57BL/6 mice and Wistar rats, where they localize in the modiolus, spiral ligament, and stria vascularis. The identity of MCs was confirmed in cochlear cryosections and flat preparations using avidin and antibodies against c-Kit/CD117, chymase, tryptase, and FcεRIα. The number of MCs decreased significantly during postnatal development, resulting in only a few MCs present in the flat preparation of the cochlea of a rat. In addition, exposure to 40 µM cisplatin for 24 h led to a significant reduction in cochlear MCs. The presence of MCs in the cochlea may shed new light on postnatal maturation of the auditory periphery and possible involvement in the ototoxicity of cisplatin. Presented data extend the current knowledge about the physiology and pathology of the auditory periphery. Future functional studies should expand and translate this new basic knowledge to clinics.
Assessment of Executive Function in Patients with Traumatic Brain Injury with the Wisconsin Card-Sorting Test
Authors: Gómez-de-Regil Lizzette
Published: October 2020

This review aimed at providing a brief and comprehensive summary of recent research regarding the use of the Wisconsin Card-Sorting Test (WCST) to assess executive function in patients with traumatic brain injury (TBI). A bibliographical search, performed in PubMed, Web of Science, Scopus, Cochrane Library, and PsycInfo, targeted publications from 2010 to 2020, in English or Spanish. Information regarding the studies’ designs, sample features and use of the WCST scores was recorded. An initial search eliciting 387 citations was reduced to 47 relevant papers. The highest proportion of publications came from the United States of America (34.0%) and included adult patients (95.7%). Observational designs were the most frequent (85.1%), the highest proportion being cross-sectional or case series studies. The average time after the occurrence of the TBI ranged from 4 to 62 years in single case studies, and from 6 weeks up to 23.5 years in the studies with more than one patient. Four studies compared groups of patients with TBI according to the severity (mild, moderate and/or severe), and in two cases, the studies compared TBI patients with healthy controls. Randomized control trials were seven in total. The noncomputerized WCST version including 128 cards was the most frequently used (78.7%). Characterization of the clinical profile of participants was the most frequent purpose (34.0%). The WCST is a common measure of executive function in patients with TBI. Although shorter and/or computerized versions are available, the original WCST with 128 cards is still used most often. The WCST is a useful tool for research and clinical purposes, yet a common practice is to report only one or a few of the possible scores, which prevents further valid comparisons across studies. Results might be useful to professionals in the clinical and research fields to guide them in assessment planning and proper interpretation of the WCST scores.
Correction: Liu, H.W.; et al. Enhanced Hsa-miR-181d/p-STAT3 and Hsa-miR-181d/p-STAT5A Ratios Mediate the Anticancer Effect of Garcinol in STAT3/5A-Addicted Glioblastoma. Cancers 2019, 11, 1888
Authors: Liu Heng-Wei , Lee Peter Mingjui , Bamodu Oluwaseun Adebayo , Su Yu-Kai , Fong Iat-Hang , Yeh Chi-Tai , Chien Ming-Hsien , Kan I-Hung , Lin Chien-Min
Published: October 2020

The authors wish to make the following corrections to this paper [...]
Water–Gas Shift Activity of Pt Catalysts Prepared by Different Methods
Authors: Bjørkan Hilde , Rønning Magnus , Venvik Hilde J. , Johannessen Tue , Holmen Anders
Published: October 2020

Platinum supported on ceria and zirconia was prepared through different preparation methods: Coprecipitation (CP), spray drying (SD), and flame spray pyrolysis (FSP). The catalysts were characterized by XRD, TPR, N2 adsorption, and H2 chemisorption, and the water–gas shift activity in the range 190–310 °C and initial stability at 300–310 °C were tested. Although the spray-dried Pt/CeO2/ZrO2 catalyst shows the highest initial activity, it deactivates rapidly at 300 °C and levels out at similar activity as the coprecipitated Pt/CeO2 and Pt/CeO2/ZrO2 within a few hours. Flame spray pyrolysis appears to be a promising preparation method concerning the stability of catalysts, although the initial activity is rather poor. High activity is related to high Pt dispersion, low reduction temperature, and small support particles. The support particle size is also much affected by the preparation method.
Interplay between the Ubiquitin Proteasome System and Ubiquitin-Mediated Autophagy in Plants
Authors: Su Tong , Yang Mingyue , Wang Pingping , Zhao Yanxiu , Ma Changle
Published: October 2020

All eukaryotes rely on the ubiquitin-proteasome system (UPS) and autophagy to control the abundance of key regulatory proteins and maintain a healthy intracellular environment. In the UPS, damaged or superfluous proteins are ubiquitinated and degraded in the proteasome, mediated by three types of ubiquitin enzymes: E1s (ubiquitin activating enzymes), E2s (ubiquitin conjugating enzymes), and E3s (ubiquitin protein ligases). Conversely, in autophagy, a vesicular autophagosome is formed that transfers damaged proteins and organelles to the vacuole, mediated by a series of ATGs (autophagy related genes). Despite the use of two completely different componential systems, the UPS and autophagy are closely interconnected and mutually regulated. During autophagy, ATG8 proteins, which are autophagosome markers, decorate the autophagosome membrane similarly to ubiquitination of damaged proteins. Ubiquitin is also involved in many selective autophagy processes and is thus a common factor of the UPS and autophagy. Additionally, the components of the UPS, such as the 26S proteasome, can be degraded via autophagy, and conversely, ATGs can be degraded by the UPS, indicating cross regulation between the two pathways. The UPS and autophagy cooperate and jointly regulate homeostasis of cellular components during plant development and stress response.
Transient Multivalent Nanobody Targeting to CD206-Expressing Cells via PH-Degradable Nanogels
Authors: Scherger Maximilian , Bolli Evangelia , Antunes Ana Rita Pombo , Arnouk Sana , Stickdorn Judith , Van Driessche Alexandra , Schild Hansjörg , Grabbe Stephan , De Geest Bruno G. , Van Ginderachter Jo A. , Nuhn Lutz
Published: October 2020

To target nanomedicines to specific cells, especially of the immune system, nanobodies can be considered as an attractive tool, as they lack the Fc part as compared to traditional antibodies and, thus, prevent unfavorable Fc-receptor mediated mistargeting. For that purpose, we have site-specifically conjugated CD206/MMR-targeting nanobodies to three types of dye-labeled nanogel derivatives: non-degradable nanogels, acid-degradable nanogels (with ketal crosslinks), and single polymer chains (also obtained after nanogel degradation). All of them can be obtained from the same reactive ester precursor block copolymer. After incubation with naïve or MMR-expressing Chinese hamster ovary (CHO) cells, a nanobody mediated targeting and uptake could be confirmed for the nanobody-modified nanocarriers. Thereby, the intact nanogels that display nanobodies on their surface in a multivalent way showed a much stronger binding and uptake compared to the soluble polymers. Based on their acidic pH-responsive degradation potential, ketal crosslinked nanogels are capable of mediating a transient targeting that gets diminished upon unfolding into single polymer chains after endosomal acidification. Such control over particle integrity and targeting performance can be considered as highly attractive for safe and controllable immunodrug delivery purposes.
Perinatal Femoral Fracture: A Ten-Year Observational Case Series Study
Authors: Pavone Vito , Vescio Andrea , Montemagno Marco , de Cristo Claudia , Lucenti Ludovico , Pavone Piero , Testa Gianluca
Published: October 2020

Background: perinatal femoral fractures (PFF) are relative rare birth-related fractures. Among treatment options, Bryant traction reported satisfactory outcomes in PFF of children under 3 years of age. The aim of this study is to assess the risk factors, diagnosis, management, and outcome in the 10-year multicentric experiences of all newborns treated for PFF in Catania city hospitals. Methods: 15,628 children, hospitalized in four neonatal units, were retrospectively reviewed. The following data were collected: gender, birth weight, gestational age, presentation, mode delivery, and fracture type according to AO Pediatric Comprehensive Classification of Long Bone Fractures (PCCF). In each case, diagnosis was achieved after the clinical examination and X-Ray exam. Each patient underwent Bryant’s skin traction of the affected limb, and was clinically followed for at least two years. Results: eight newborns were included in the study (five males). The average birth weight was 2.656 kg with a gestational age of 37.5 weeks; 4 cases were preterm birth; 5 patients had a cephalic presentation. According to the AO PCCF classification, three fractures were ranked 32-D/4.1 and five were 32-D/5.1. The entire cohort had an excellent outcome. Conclusions: prematurity, low birth weight, and caesarean section could be PFF risk factors. Bryant’s skin traction is an effective option to achieve an excellent outcome.
Participation in Everyday Activities of Children with and without Neurodevelopmental Disorders: A Cross-Sectional Study in Spain
Authors: Blanco-Martínez Nerea , Delgado-Lobete Laura , Montes-Montes Rebeca , Ruiz-Pérez Nuria , Ruiz-Pérez Marcos , Santos-del-Riego Sergio
Published: October 2020

Children with neurodevelopmental disorders (NDDs) often report significant difficulties performing activities of daily living (ADLs), which may restrict their daily participation. The aim of this study was to investigate the differences in ADLs participation between children with NDDs and typically developing (TD) children, and to explore the associations between different daily participation contexts. A cross-sectional study was conducted that included twenty children with a medical diagnosis of an NDD and 26 sex- and age-matched TD controls. The daily participation across home, community, school, and instrumental living activities was measured using the Child and Adolescent Scale of Participation (CASP). The results show that children with NDDs engaged in lower participation in all CASP contexts (Δ = 1.7–5.5, p < 0.001) and had a significantly higher prevalence of moderate or severe restricted participation than their TD peers (OR = 23.4, 95% CI = 3.6–154.2, p < 0.001). Additionally, a strong association was found between the different contexts of participation (r = 0.642–0.856). Overall, the children with NDDs experienced significant participation restrictions on their daily activities. This study adds to the growing evidence showing that intervention strategies in this population should adopt a participation-oriented approach.
Effect of Frequency Content of Earthquake on the Seismic Response of Interconnected Electrical Equipment
Authors: Salman Kashif , Gook Cho Sung
Published: October 2020

To ensure the stable operation of safety-related nuclear power plant (NPP) equipment, they are tested by following the seismic qualification procedures. The in-cabinet response spectrum (ICRS) is used to test the mounted components. However, the ICRS varies significantly with the number of uncertainties that include (1) loaded and unloaded condition of the cabinets, (2) the number of connected cabinets (grouping effects), and (3) higher frequency contents in the seismic inputs. This study focuses on the ICRS generation and alteration induced due to the listed uncertainties. A prototype of an electrical cabinet was experimentally examined. Followed by the numerical modeling of the cabinet, the seismic analysis for the group of cabinets was performed using artificial ground motion compatible with the standard design spectrum and the real accelerograms of high and low frequency contents. The seismic response using finite element (FE) analysis manifests (1) natural frequency of loaded cabinets reduced due to the in-cabinet components while for the unloaded cabinets it increased significantly, (2) a consistent reduction in ICRS due to the grouping effect was recorded when excited by the lower-frequency motion, while it was amplified dramatically due to high-frequency pulses. Interconnected cabinets under the low-frequency input motions have a significant reduction of 50% in the ICRS that corresponds to the higher stiffness of the cabinets, while a 100% increase under the high frequency of ground motion was obtained. High frequency of ground motion, usually above 10 Hz, can cause the interconnected cabinets to resonate as the natural frequency of these equipment lies in this range.
Thermal Stress Analysis of Environmental Barrier Coatings Considering Interfacial Roughness
Authors: Fang Guangwu , Ren Jiacheng , Shi Jian , Gao Xiguang , Song Yingdong
Published: October 2020

A numerical analysis of the effect of roughness interface on the thermal stress in the environmental barrier coatings for ceramic matrix composites was performed. Based on the concept of representative volume elements, a micromechanical finite element model of the coated composites was established. The rough interfaces between the coating layers were described using sine curves. The cooling process after preparation and the typical service conditions for the CMCs component were simulated, respectively. The results show that the rough interface has little effect on the temperature distribution along the depth direction for the studied T/EBC coatings for SiC/SiC composites. The stress concentration occurs at the rough EBC/BC interface, which is prone to cause delamination cracking. Under typical service conditions, the high temperature can eliminate part of the thermal residual stress. Meanwhile, the thermal gradient will cause large thermal stress in the TBC layer and the stress will result in surface cracks. The stress concentrations appear at the peaks and valleys of rough interfaces. The variation range of thermal stress increases with the roughness amplitude and decreases with the wavelength.
Use of Silver Nanoparticle–Gelatin/Alginate Scaffold to Repair Skull Defects
Authors: Zhao Yang , Liu Jun , Zhang Mingyue , He Jia , Zheng Bowen , Liu Fan , Zhao Zhenjin , Liu Yi
Published: October 2020

The major objectives of this study were to investigate the effects of silver nanoparticles– gelatin (AgNPs) on the physical and chemical properties of gelatin/alginate (Gel/Alg) scaffolds and the bone-promoting effect of AgNP–Gel/Alg scaffolds. Gel/Alg scaffolds consisting of 0 μM, 200 μM, 400 μM, and 600 μM AgNPs were prepared. SEM was used to evaluate the physical properties of the scaffolds. A CCK-8 assay was performed to determine the cell proliferation activity, and Micro-CT and histological analysis were used to assess the osteogenic effect. The pore size, porosity, and the water absorption and degradation rates of AgNP–Gel/Alg scaffolds were found to be increased compared with those of Gel/Alg scaffolds (control group). CCK-8 showed that cell proliferation activity in the 200 μM group was significantly higher than that in the control group. Micro-CT analysis showed that there was more new bone around AgNP–Gel/Alg than the control group, and the amount of bone formation in the 200 μM group was significantly higher than that in the other groups. Masson staining showed that numerous collagen fibers had proliferated around the AgNP–Gel/Alg scaffold and tended to thicken over time. AgNP–Gel/Alg scaffolds promoted the repair of skull defects in New Zealand rabbits and exerted a marked osteogenic effect in vivo. The 200 μM AgNP–Gel/Alg scaffold was shown to be more suitable for bone tissue engineering materials.
Nanostructures on Sapphire Surfaces Induced by Metal Impurity Assisted Ion Beam
Authors: Bi Qian , Chen Zhili , Liu Yuzhao , Tang Li , Xi Yingxue , Liu Weiguo
Published: October 2020

The metal impurity assisted ion beam technology has shown its uniqueness and effectiveness in the formation and precise control of nanostructures on the surface of materials. Hence, the investigation in this area is vital. The morphology evolution of self-organized nanostructures induced by Fe co-deposition assisted Ar+ ion beam sputtering at a different distance from the impurity target was investigated on sapphire, using atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS). We also investigated the role of metal impurities on sapphire ripple formation. Experiments were carried out at an oblique angle of incidence 65° with constant ion beam current density 487 μA/cm2 and the erosion duration of 60 min at room temperature (20 °C). The introduction of Fe impurity increased the longitudinal height and roughness of the surface nanostructures. Moreover, the amounts of Fe deposited on the surface decreased with increasing distance, and the morphology of the smooth sapphire surface demonstrated a strong distance dependence. Differences in surface morphology were attributed to changes in metal impurity concentration. With an increase of impurity target distance, island-like structures gradually evolved into continuous ripples. At the same time, the orderliness of nanostructures was enhanced, the longitudinal height gradually decreased, while the spatial frequency was unchanged. In addition, there were very few metal impurities on the etched sample. During the ion beam sputtering process, island-like structures promoted the growth of ripples but destroyed their orderliness.
Numerical Investigation on Dynamic Response of RC T-Beams Strengthened with CFRP under Impact Loading
Authors: Zhao Huiling , Kong Xiangqing , Fu Ying , Gu Yihan , Wang Xuezhi
Published: October 2020

To precisely evaluate the retrofitting effectiveness of Carbon Fiber Reinforced Plastic (CFRP) sheets on the impact response of reinforced concrete (RC) T-beams, a non-linear finite element model was developed to simulate the structural response of T-beams with CFRP under impact loads. The numerical model was firstly verified by comparing the numerical simulation results with the experimental data, i.e., impact force, reaction force, and mid-span displacement. The strengthening effect of CFRP was analyzed from the section damage evaluation. Then the impact force, mid-span displacement, and failure mode of CFRP-strengthened RC T-beams were studied in comparison with those of un-strengthened T-beams. In addition, the influence of the impact resistance of T-beams strengthened with FRP was investigated in terms of CFRP strengthening mode, CFRP strengthening sizes, CFRP layers and FRP material types. The numerical simulation results indicate that the overall stiffness of the T-beams was improved significantly due to external CFRP strips. Compared with the un-strengthened beam, the maximum mid-span displacement of the CFRP-strengthened beam was reduced by 7.9%. Additionally, the sectional damage factors of the whole span of the CFRP-strengthened beam were reduced to less than 0.3, indicating that the impact resistance of the T-beams was effectively enhanced.
Load Control by Demand Side Management to Support Grid Stability in Building Clusters
Authors: Hall Monika , Geissler Achim
Published: October 2020

Increasing numbers of photovoltaic systems and heat pumps in existing building clusters can lead to an overload of the associated electric grid substations. Based on a multi-agent-based simulation of three building cluster types the impact of building flexibility in regard to the residual substation load is studied. Each building announces its available flexibility, e.g., “heat pump can be switched off/on”. A cluster master coordinator evaluates the incoming offers and decides which offers are accepted in regard to the substation’s capacity utilization. The goal is to honour the substation’s limit by shifting the residual load. This paper presents results from three typical urban building clusters for different penetration scenarios in regard to heat pumps, photovoltaic systems, batteries and electric vehicles. It is shown that in the studied building clusters a high penetration of heat pumps and photovoltaic systems can violate the existing substation’s limits, regardless of the efforts by the master coordinator. Batteries of typical capacities cannot reduce the peak residual load. The load shifting options of the master coordinator are limited.
The Pluriversality of Efforts to Reduce Deforestation in Brazil over the Past Decade: An Analysis of Policy Actors’ Perceptions
Authors: Gebara Maria Fernanda , Gallo Patrícia , Brites Alice , Lima Guilherme , Micheletti Tatiane
Published: October 2020

Brazil offers a complex and unique example of tropical landscapes. The country has considerably decreased deforestation since 2004, but Reducing Emissions from Deforestation and Forest Degradation (REDD+) is arguably under question, both as a concept and as a tool to reduce greenhouse gas (GHG) emissions, as deforestation levels have increased over the last five years. This article investigates how different policy actors have perceived REDD+ over time, how they have engaged in REDD+ efforts over the past decade, and how REDD+ implementation in Brazil should move forward accordingly. We analyzed qualitative data from semi-structured interviews and actors’ “stances”, i.e., their positions—with regards to key issues connected to REDD+ governance and its challenges—over three different time periods (Phase 1: 2010–2011, Phase 2: 2015–2016, and Phase 3: 2019), so as to identify the practical implications of these diverse interpretations. We argue that the way actors perceive REDD+ is intimately related to the way they interpret and assign meanings towards it and, in consequence, the way they coordinate REDD+-related practices and efforts. We focus on the link between perceptions and efforts so as to comprehend the relevance that different interpretations have to both the concept and implementation of REDD+ in Brazil. Our analysis concentrates on the potential to improve the coordination and integration of REDD+ implementation and diverse actors’ efforts with regards to REDD+ activities. Results suggest that actors’ perceptions of REDD+ generated a plurality of meanings, highlighting a range of dialectical and ontological interactions that have, in turn, resulted in multiple REDD+ interpretations. Findings highlight that different actors have the same interests when it comes to their organizational efforts to reduce emissions from deforestation and forest degradation, suggesting that there is room for a better coordination of efforts towards this end, as well as increased collaboration.
Radial Growth Responses to Climate of Pinus yunnanensis at Low Elevations of the Hengduan Mountains, China
Authors: Sun Lian , Cai Yanpeng , Zhou Yang , Shi Shiyuan , Zhao Yesi , Gunnarson Björn E. , Jaramillo Fernando
Published: October 2020

The relationship between climate and forest is critical to understanding the influence of future climate change on terrestrial ecosystems. Research on trees at high elevations has uncovered the relationship in the Hengduan Mountains region, a critical biodiversity hotspot area in southwestern China. The relationship for the area at low elevations below 2800 m a.s.l. in the region remains unclear. In this study, we developed tree ring width chronologies of Pinus yunnanensis Franch. at five sites with elevations of 1170–1725 m in this area. Monthly precipitation, relative humidity, maximum/mean/minimum air temperature and the standardized precipitation evapotranspiration index (SPEI), a drought indicator with a multi-timescale, were used to investigate the radial growth-climate relationship. Results show that the growth of P. yunnanensis at different sites has a similar response pattern to climate variation. Relative humidity, precipitation, and air temperature in the dry season, especially in its last month (May), are critical to the radial growth of trees. Supplemental precipitation amounts and reduced mean or maximum air temperature can promote tree growth. The high correlations between chronologies and SPEI indicate that the radial growth of trees at the low elevations of the region is significantly limited by the moisture availability. Precipitation in the last month of the previous wet season determines the drought regime in the following dry seasons. In spite of some differences in the magnitudes of correlations in the low-elevation area of the Hengduan Mountains region, chronologies generally matched well with each other at different elevations, and the differences are not evident with the change in elevation.
The Effect of Roasting on the Protein Profile and Antiradical Capacity of Flaxseed Meal
Authors: Waszkowiak Katarzyna , Mikołajczak Beata
Published: October 2020

Roasting is more and more often used as a pre-treatment of flaxseeds. However, the process can influence flaxseed proteins that may be crucial for their properties. The aim of this research was to study changes in the electrophoretic protein profile (SDS-PAGE) and the antiradical capacity of flaxseed meals after roasting. The roasting temperature (160, 180, and 200 °C) and flaxseed cultivars (golden and brown seed) were factors including in the study. The free (F-MRP) and bound-to-protein (B-MRP) Maillard reaction products were also analyzed. The most significant changes in the SDS-PAGE protein profiles of roasted seeds of each of the tested flax cultivars were observed for the 13 kDa protein fraction (decrease) and for the 19 kDa and 17 kDa fractions (increase). The research revealed a significant correlation between the roasting temperature and B-MRP content, and changes in the percentage share of those three protein fractions. The antiradical capacity of roasted flaxseeds decreased, as compared with untreated seeds. After roasting at 200 °C the antiradical capacity of flaxseeds improved slightly, probably due to the MRP formation, but it was still significantly lower than that of the raw seeds. The research provides novel information about key protein fractions that seem to be important changing during heat treatment.
The Use of Less Conventional Meats or Meat with High pH Can Lead to the Growth of Undesirable Microorganisms during Natural Meat Fermentation
Authors: Charmpi Christina , Van Reckem Emiel , Sameli Nikoleta , Van der Veken David , De Vuyst Luc , Leroy Frédéric
Published: October 2020

The bacterial communities that are established during natural meat fermentation depend on the processing conditions and the type of meat substrate used. Six pork samples of variable quality (reflected in pH values) and six less conventional meats (beef, horse, hare, wild deer, wild duck, and wild boar) were naturally fermented under controlled conditions in model systems. The development of lactic acid bacteria (LAB), coagulase-negative staphylococci (CNS), and enterobacteria was followed using culture-dependent techniques and (GTG)5-PCR fingerprinting of genomic DNA from the isolates obtained. Taken together, Latilactobacillus sakei was the most abundant LAB species, although Latilactobacillus curvatus was more manifest in high-pH pork. Within staphylococci, common species were encountered (i.e., Staphylococcus equorum, Staphylococcus saprophyticus, and Staphylococcus xylosus), although some atypical ones (i.e., Staphylococcus succinus) were also recovered. Within enterobacteria, Serratia spp. prevailed in more acidic pork batches and in beef, whereas Hafnia spp. prevailed in game meat fermentations. Enterobacterial counts were particularly high in fermentations with low acidity, namely for some pork batches, hare, wild duck, and wild boar. These findings should be considered when naturally fermented meat products are manufactured, as the use of game meat or meat with high pH can give rise to safety concerns.
Predicting the Content of 20 Minerals in Beef by Different Portable Near-Infrared (NIR) Spectrometers
Authors: Patel Nageshvar , Toledo-Alvarado Hugo , Cecchinato Alessio , Bittante Giovanni
Published: October 2020

The aim of this study was to test the predictability of a detailed mineral profile of beef using different portable near-infrared spectrometers (NIRS). These devices are rapid, chemical waste-free, cheap, nondestructive tools that can be used directly on the meat surface in the work environment without the need to take samples. We compared a transportable Visible-NIRS (weight 5.6 kg; wavelength 350–1830 nm), a portable NIRS (2.0 kg; 950–1650 nm), and a hand-held Micro-NIRS (0.06 kg; 905–1649 nm) to predict the contents of 20 minerals (measured by ICP-OES) in 178 beef samples (Longissimus thoracis muscle) using different mathematical pretreatments of the spectra and partial least square regressions. The externally validated results show that Fe, P, Mg, S, Na, and Pb have some potential for prediction with all instruments (R2VAL: 0.40–0.83). Overall, the prediction performances of the three instruments were similar, although the smallest (Micro-NIRS) exhibited certain advantages.
By-Products from Winemaking and Olive Mill Value Chains for the Enrichment of Refined Olive Oil: Technological Challenges and Nutraceutical Features
Authors: Macaluso Monica , Bianchi Alessandro , Sanmartin Chiara , Taglieri Isabella , Venturi Francesca , Testai Lara , Flori Lorenzo , Calderone Vincenzo , De Leo Marinella , Braca Alessandra , Ciccone Valerio , Donnini Sandra , Guidi Luca , Zinnai Angela
Published: October 2020

A growing body of literature is available about the valorization of food by-products to produce functional foods that combine the basic nutritional impact with the improvement of the health status of consumers. In this context, this study had two main objectives: (i) An innovative multistep extraction process for the production of a refined olive oil enriched with phenolic compounds (PE-ROO) extracted from olive pomace, olive leaves, or grape marc was presented and discussed. (ii) The most promising PE-ROOs were selected and utilized in in vitro and in vivo trials in order to determine their effectiveness in the management of high fat diet-induced-metabolic syndrome and oxidative stress in rats. The best results were obtained when olive leaves were used as source of phenols, regardless of the chemical composition of the solvent utilized for the extraction. Furthermore, while ethanol/hexane mixture was confirmed as a good solvent for the extraction of phenols compounds soluble in oil, the mix ROO/ethanol also showed a good extracting power from olive leaves. Besides, the ROO enriched with phenols extracted from olive leaves revealed an interesting beneficial effect to counteract high fat diet-induced-metabolic disorder and oxidative stress in rats, closely followed by ROO enriched by utilizing grape marc.
Exercise-Induced Myokines can Explain the Importance of Physical Activity in the Elderly: An Overview
Authors: Kwon Jenny Hyosun , Moon Kyoung Min , Min Kyueng-Whan
Published: October 2020

Physical activity has been found to aid the maintenance of health in the elderly. Exercise-induced skeletal muscle contractions lead to the production and secretion of many small proteins and proteoglycan peptides called myokines. Thus, studies on myokines are necessary for ensuring the maintenance of skeletal muscle health in the elderly. This review summarizes 13 myokines regulated by physical activity that are affected by aging and aims to understand their potential roles in metabolic diseases. We categorized myokines into two groups based on regulation by aerobic and anaerobic exercise. With aging, the secretion of apelin, β-aminoisobutyric acid (BAIBA), bone morphogenetic protein 7 (BMP-7), decorin, insulin-like growth factor 1 (IGF-1), interleukin-15 (IL-15), irisin, stromal cell-derived factor 1 (SDF-1), sestrin, secreted protein acidic rich in cysteine (SPARC), and vascular endothelial growth factor A (VEGF-A) decreased, while that of IL-6 and myostatin increased. Aerobic exercise upregulates apelin, BAIBA, IL-15, IL-6, irisin, SDF-1, sestrin, SPARC, and VEGF-A expression, while anaerobic exercise upregulates BMP-7, decorin, IGF-1, IL-15, IL-6, irisin, and VEGF-A expression. Myostatin is downregulated by both aerobic and anaerobic exercise. This review provides a rationale for developing exercise programs or interventions that maintain a balance between aerobic and anaerobic exercise in the elderly.
The Impact of COVID-19 on Telemedicine Utilization Across Multiple Service Lines in the United States
Authors: Betancourt Jose A. , Rosenberg Matthew A. , Zevallos Ashley , Brown Jon R. , Mileski Michael
Published: October 2020

The impact of COVID-19 on the U.S. healthcare industry cannot be overstated. Telemedicine utilization increased overnight as all healthcare providers rushed to implement this delivery model to ensure accessibility and continuity of patient care. Our research objective was to determine measures that were implemented to accommodate community and individual patient needs to afford access to critical services and to maintain safety standards. We analyzed literature since 2016 from two databases using Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). We compared observations, themes, service lines addressed, issues identified, and interventions requiring in-person care. From 44 articles published, we identified ten effectiveness themes overall and drew conclusions on service line successes. COVID-19 has caused rapid expansion in telemedicine. Necessary and required changes in access, risk mitigation, the need for social distancing, compliance, cost, and patient satisfaction are a few of the driving factors. This review showcased the healthcare industry’s ability to rapidly acclimate and change despite the pervasive spread of COVID-19 throughout the U.S. Although imperfect, unique responses were developed within telemedicine platforms to mitigate disruptions broadly and effectively in care and treatment modalities.