bluuebunny/crossref_metadata_2025 huggingface dataset:
Open Science Expectations for Simulation-Based Research
Authors: Mullendore Gretchen L. , Mayernik Matthew S. , Schuster Douglas C.
Published: November 2021

There is strong agreement across the sciences that replicable workflows are needed for computational modeling. Open and replicable workflows not only strengthen public confidence in the sciences, but also result in more efficient community science. However, the massive size and complexity of geoscience simulation outputs, as well as the large cost to produce and preserve these outputs, present problems related to data storage, preservation, duplication, and replication. The simulation workflows themselves present additional challenges related to usability, understandability, documentation, and citation. These challenges make it difficult for researchers to meet the bewildering variety of data management requirements and recommendations across research funders and scientific journals. This paper introduces initial outcomes and emerging themes from the EarthCube Research Coordination Network project titled “What About Model Data? - Best Practices for Preservation and Replicability,” which is working to develop tools to assist researchers in determining what elements of geoscience modeling research should be preserved and shared to meet evolving community open science expectations.

Specifically, the paper offers approaches to address the following key questions:

• How should preservation of model software and outputs differ for projects that are oriented toward knowledge production vs. projects oriented toward data production?

• What components of dynamical geoscience modeling research should be preserved and shared?

• What curation support is needed to enable sharing and preservation for geoscience simulation models and their output?

• What cultural barriers impede geoscience modelers from making progress on these topics?
IL-18 Mediates Vascular Calcification Induced by High-Fat Diet in Rats With Chronic Renal Failure
Authors: Zhang Yinyin , Zhang Kun , Zhang Yuling , Zhou Lingqu , Huang Hui , Wang Jingfeng
Published: November 2021

Objective: Vascular calcification (VC) is an important predictor of cardiovascular morbidity and mortality in patients with chronic renal failure (CRF). It is well-known that obesity and metabolic syndrome (OB/MS) predicts poor prognosis of CRF patients. However, the influence of OB/MS on VC in CRF patients isn't clear. IL-18 mediates OB/MS-related inflammation, but whether IL-18 is involved in OB/MS -mediated VC in CRF patients hasn't been studied. In this study, it was explored that whether OB/MS caused by high-fat diet (HFD) can affect the level of serum IL-18 and aggravate the degree of VC in CRF rats. Furthermore, it was studied that whether IL-18 induces rat vascular smooth muscle cells (VSMCs) calcification by activating the MAPK pathways.

Approach: The rats were randomly assigned to the sham-operated, CRF and CRF + HFD groups. CRF was induced by 5/6 nephrectomy. Serum IL-18 levels and aortic calcification indicators were compared in each group. Primary rat VSMCs calcification were induced by β-glycerophosphate and exposed to IL-18. VSMCs were also treated with MAPK inhibitors.

Results: The weight, serum levels of hsCRP, TG and LDL-C in CRF + HFD group were significantly higher than those in sham-operated and CRF groups (p < 0.05). Compared with the sham-operated group, the calcium content and the expression of BMP-2 of aorta in CRF and CRF + HFD groups were significantly increased (p < 0.05). Moreover, the calcium content and the expression of BMP-2 of aorta in CRF + HFD group was significantly higher than those in CRF group (p < 0.05). And the serum IL-18 level was positively correlated with aortic calcium content. It was also found that p38 inhibitor SB203580 can suppress the VSMCs calcification and osteoblast phenotype differentiation induced by IL-18. But the JNK inhibitor SP600125 can't suppress the VSMCs calcification and osteoblast phenotype differentiation induced by IL-18.

Conclusions: These findings suggest that obesity-related inflammation induced by high-fat diet could exacerbate VC in CRF rats. Furthermore, serum IL-18 level had a positive correlation with the degree of VC. It is also found that IL-18 promoted osteogenic differentiation and calcification of rat VSMCs via p38 pathway activation.
Incidence and Characterization of Concealed Cardiac Amyloidosis Among Unselected Elderly Patients Undergoing Post-mortem Examination
Authors: Porcari Aldostefano , Bussani Rossana , Merlo Marco , Varrà Guerino Giuseppe , Pagura Linda , Rozze Davide , Sinagra Gianfranco
Published: November 2021

Background: The prevalence of cardiac amyloidosis (CA) is unknown.

Aims and Methods: We sought to (a) determine the prevalence of CA in unselected patients ≥75 years undergoing autopsy, (b) characterize cardiological profiles of CA and non-CA patients by providing clinical-histological correlations, and (c) compare their cardiological profiles. After dedicated staining, the localization (interstitial or vascular) and the distribution (non-diffuse or diffuse) of amyloid deposition were analyzed. Cardiological data at last evaluation were retrospectively assessed for the presence of CA red-flags.

Results: CA (50% light chains, 50% transthyretin) was found in 43% (n = 24/56) of the autopsied hearts. Atria were involved in 96% of cases. Amyloid localized both at the perivascular and interstitial levels (95.5 and 85%, respectively) with a slightly predominant non-diffuse distribution (58% of cases). Compared to the other patients, CA patients had a more frequent history of heart failure (HF) (79 vs. 47%, p = 0.014), advanced NYHA functional class (III-IV 25 vs. 6%, p = 0.047), atrial fibrillation (68 vs. 36%, p = 0.019), discrepancy between QRS voltage and left ventricular (LV) thickness (70 vs. 12%, p < 0.001), thicker LV walls (15 vs. 11 mm, p < 0.001), enlarged left atrium (49 vs. 42 mm, p = 0.019) and restrictive filling pattern (56 vs. 19%, p = 0.020). The presence of right ventricular amyloidosis seemed to identify hearts with a higher amyloid burden. Among the CA patients, >30% had ≥3 echocardiographic red-flags of disease.

Conclusion: CA can be found in 43% of autopsied hearts from patients ≥75 years old, especially in patients with HF, LV hypertrophy and atrial fibrillation.
Single-Cell Transcriptome Profiles Reveal Fibrocytes as Potential Targets of Cell Therapies for Abdominal Aortic Aneurysm
Authors: Li Bolun , Song Xiaomin , Guo Wenjun , Hou Yangfeng , Hu Huiyuan , Ge Weipeng , Fan Tianfei , Han Zhifa , Li Zhiwei , Yang Peiran , Gao Ran , Zhao Hongmei , Wang Jing
Published: November 2021

Abdominal aortic aneurysm (AAA) is potentially life-threatening in aging population due to the risk of aortic rupture and a lack of optimal treatment. The roles of different vascular and immune cells in AAA formation and pathogenesis remain to be future characterized. Single-cell RNA sequencing was performed on an angiotensin (Ang) II-induced mouse model of AAA. Macrophages, B cells, T cells, fibroblasts, smooth muscle cells and endothelial cells were identified through bioinformatic analyses. The discovery of multiple subtypes of macrophages, such as the re-polarization of Trem2+Acp5+ osteoclast-like and M2-like macrophages toward the M1 type macrophages, indicates the heterogenous nature of macrophages during AAA development. More interestingly, we defined CD45+COL1+ fibrocytes, which was further validated by flow cytometry and immunostaining in mouse and human AAA tissues. We then reconstituted these fibrocytes into mice with Ang II-induced AAA and found the recruitment of these fibrocytes in mouse AAA. More importantly, the fibrocyte treatment exhibited a protective effect against AAA development, perhaps through modulating extracellular matrix production and thus enhancing aortic stability. Our study reveals the heterogeneity of macrophages and the involvement of a novel cell type, fibrocyte, in AAA. Fibrocyte may represent a potential cell therapy target for AAA.
Cardiac Care of Non-COVID-19 Patients During the SARS-CoV-2 Pandemic: The Pivotal Role of CCTA
Authors: Conte Edoardo , Mushtaq Saima , Mancini Maria Elisabetta , Annoni Andrea , Formenti Alberto , Muscogiuri Giuseppe , Gaudenzi Asinelli Margherita , Gigante Carlo , Collet Carlos , Sonck Jeroen , Guglielmo Marco , Baggiano Andrea , Cosentino Nicola , Denora Marialessia , Belmonte Marta , Agalbato Cecilia , Esposito Andrea Alessandro , Assanelli Emilio , Bartorelli Antonio L. , Pepi Mauro , Pontone Gianluca , Andreini Daniele
Published: November 2021

Aim: The aim of this study is to evaluate the potential use of coronary CT angiography (CCTA) as the sole available non-invasive diagnostic technique for suspected coronary artery disease (CAD) during the coronavirus disease 2019 (COVID-19) pandemic causing limited access to the hospital facilities.

Methods and Results: A consecutive cohort of patients with suspected stable CAD and clinical indication to non-invasive test was enrolled in a hub hospital in Milan, Italy, from March 9 to April 30, 2020. Outcome measures were obtained as follows: cardiac death, ST-elevation myocardial infarction (STEMI), non-ST-elevation myocardial infarction (NSTEMI), and unstable angina. All the changes in medical therapy following the result of CCTA were annotated. A total of 58 patients with a mean age of 64 ± 11 years (36 men and 22 women) were enrolled. CCTA showed no CAD in 14 patients (24.1%), non-obstructive CAD in 30 (51.7%) patients, and obstructive CAD in 14 (24.1%) patients. Invasive coronary angiography (ICA) was considered deferrable in 48 (82.8%) patients. No clinical events were recorded after a mean follow-up of 376.4 ± 32.1 days. Changes in the medical therapy were significantly more prevalent in patients with vs. those without CAD at CCTA.

Conclusion: The results of the study confirm the capability of CCTA to safely defer ICA in the majority of symptomatic patients and to correctly identify those with critical coronary stenoses necessitating coronary revascularization. This characteristic could be really helpful especially when the hospital resources are limited
Statins in Non-alcoholic Steatohepatitis
Authors: Torres-Peña Jose D. , Martín-Piedra Laura , Fuentes-Jiménez Francisco
Published: November 2021

Non-alcoholic fatty liver disease (NAFLD) is the primary cause of chronic liver disease. The range is extensive, including hepatocellular carcinoma, cirrhosis, fibrosis, fatty liver, and non-alcoholic steatohepatitis (NASH). NASH is a condition related to obesity, overweight, metabolic syndrome, diabetes, and dyslipidemia. It is a dynamic condition that can regress to isolated steatosis or progress to fibrosis and cirrhosis. Statins exert anti-inflammatory, proapoptotic, and antifibrotic effects. It has been proposed that these drugs could have a relevant role in NASH. In this review, we provide an overview of current evidence, from mechanisms of statins involved in the modulation of NASH to human trials about the use of statins to treat or attenuate NASH.
Retrospective Analysis and Forecasted Economic Impact of a Virtual Cardiac Rehabilitation Program in a Third-Party Payer Environment
Authors: Harzand Arash , Weidman Aaron C. , Rayl Kenneth R. , Adesanya Adelanwa , Holmstrand Ericka , Fitzpatrick Nicole , Vathsangam Harshvardhan , Murali Srinivas
Published: November 2021

Background: Participation in cardiac rehabilitation (CR) is recommended for all patients with coronary artery disease (CAD) following hospitalization for acute coronary syndrome or stenting. Yet, few patients participate due to the inconvenience and high cost of attending a facility-based program, factors which have been magnified during the ongoing COVID pandemic. Based on a retrospective analysis of CR utilization and cost in a third-party payer environment, we forecasted the potential clinical and economic benefits of delivering a home-based, virtual CR program, with the goal of guiding future implementation efforts to expand CR access.

Methods: We performed a retrospective cohort study using insurance claims data from a large, third-party payer in the state of Pennsylvania. Primary diagnostic and procedural codes were used to identify patients admitted for CAD between October 1, 2016, and September 30, 2018. Rates of enrollment in facility-based CR, as well as all-cause and cardiovascular hospital readmission and associated costs, were calculated during the 12-months following discharge.

Results: Only 37% of the 7,264 identified eligible insured patients enrolled in a facility-based CR program within 12 months, incurring a mean delivery cost of $2,922 per participating patient. The 12-month all-cause readmission rate among these patients was 24%, compared to 31% among patients who did not participate in CR. Furthermore, among those readmitted, CR patients were readmitted less frequently than non-CR patients within this time period. The average per-patient cost from hospital readmissions was $30,814 per annum. Based on these trends, we forecasted that adoption of virtual CR among patients who previously declined CR would result in an annual cost savings between $1 and $9 million in the third-party healthcare system from a combination of increased overall CR enrollment and fewer hospital readmissions among new HBCR participants.

Conclusions: Among insured patients eligible for CR in a third-party payer environment, implementation of a home-based virtual CR program is forecasted to yield significant cost savings through a combination of increased CR participation and a consequent reduction in downstream healthcare utilization.
Digital Health Coaching for Type 2 Diabetes: Randomized Controlled Trial of Healthy at Home
Authors: Azelton Kimberly R. , Crowley Aidan P. , Vence Nicholas , Underwood Karin , Morris Gerald , Kelly John , Landry Matthew J.
Published: November 2021

Digital health coaching is an intervention for type 2 diabetes mellitus (T2DM) that has potential to improve the quality of care for patients. Previous research has established the efficacy of digital interventions for behavior change. This pilot study addresses a research gap in finding effective and accessible behavioral interventions for under-resourced individuals with T2DM. We examined the impact of Healthy at Home, a 12-week phone and SMS-based (short message service) digital health coaching program, on insulin resistance which is an upstream marker for T2DM progression. We compared this intervention to usual diabetic care in a family medicine residency clinic in a randomized controlled trial. Digital health coaching significantly improved participants' calculated Homeostatic Model Assessment for Insulin Resistance (HOMA2-IR) by −0.9 ± 0.4 compared with the control group (p = 0.029). This significance remained after controlling for years diagnosed with T2DM, enrollment in Medicaid, access to food, baseline stage of change, and race (p = 0.027). Increasing access to digital health coaching may lead to more effective control of diabetes for under-resourced patients. This study demonstrates the potential to implement a personalized, scalable, and effective digital health intervention to treat and manage T2DM through a lifestyle and behavioral approach to improve clinical outcomes (http://clinicaltrials.gov, NCT04872647).
Gabbro Discovery in Discovery Deep: First Plutonic Rock Samples From the Red Sea Rift Axis
Authors: Follmann Jörg , van der Zwan Froukje M. , Preine Jonas , Hübscher Christian , Bousquet Romain , Augustin Nico
Published: November 2021

Plutonic rocks such as gabbros provide information on magmatic and tectonic processes which occur beneath a mid-ocean rift axis as well as on the formation of the oceanic crust. Igneous rocks, reported from the Red Sea Rift valley, have been limited to extrusive basalts so far. The only deeper crustal rocks found in the Red Sea area are from the rift flanks and are interpreted as late-stage continental rift magmatism. Here, we present the geochemistry of the first recovered gabbro fragments from the axis of the Red Sea Rift, sampled from a crater structure within the brine-filled Discovery Deep at the axis of the Red Sea Rift. Petrology and geochemistry show characteristics of a typical mid-ocean ridge gabbro formed at shallow crystallization depth. Clinopyroxene core mineral data fall within two groups, thus pointing to a multiphased magmatic history, including different magma batches and a joint late-stage fractional crystallization. Geobarometry, based on clinopyroxene cores, suggests lower crystallization pressures than similar geobarometric data reported for gabbroic samples from Zabargad (8–9 kbar) and Brother’s Islands (2.5–3.5 kbar) at the rift flanks. However, based on the evolved whole rock composition, its multiphase history, the thickness of the crust, the current location of the samples, and the uncertainties in the barometer, geobarometric estimates for the samples are likely overestimated. Instead, we propose that these rock fragments originate from the upper part of a fully developed oceanic crust in the central Red Sea Rift. High-resolution bathymetry and sparker seismic data reveal that the Discovery Deep is characterized by a significant normal fault and a strong reflector near the rift axis, which we interpret as a potential sill intrusion in an approximate depth of 400 m. Based on the lack of progressive alteration and the sampling location within a sediment-free crater structure, we interpret that the emplacement of the gabbros has to be geologically recent. We interpret the gabbro either as a xenolith transported by the eruptive volcanism that formed the crater, potentially related to the sill intrusion visible at depth, or as intrusive gabbro, which was uplifted and deposited in a talus fan by the adjacent normal fault, exposed by the formation of the volcanic crater.
Pastoralism and Millet Cultivation During the Bronze Age in the Temperate Steppe Region of Northern China
Authors: Zhang Yaping , Zhang Ying , Hu Songmei , Zhou Xinying , Liu Linjing , Liu Junchi , Zhao Keliang , Li Xiaoqiang
Published: November 2021

Eastern and Western Asia were important centers for the domestication of plants and animals and they developed different agricultural practices and systems. The timing, routeway and mechanisms of the exchanges between the two centers have long been important scientific issues. The development of a mixed pastoral system (e.g., with the rearing of sheep, goats and cattle) and millet cultivation in the steppe region of northern China was the result of the link between the two cultures. However, little detailed information is available about the precise timing and mechanisms involved in this mixture of pastoralism and millet cultivation. To try to address the issue, we analyzed the pollen, fungal spores and phytolith contents of soil samples from the Bronze Age Zhukaigou site in the steppe area of North China, which was combined with AMS 14C dating of charcoal, millet and animal bones. A mixed pastoralism and millet agricultural system appeared at the site between 4,000 and 3,700 cal yr BP, and the intensity of animal husbandry increased in the later stage of occupation. Published data indicate that domestic sheep/goats appeared across a wide area of the steppe region of northern China after ∼4,000 cal yr BP. A comparison of records of sheep/goat rearing and paleoclimatic records from monsoon area in China leads us to conclude that the mixture of pastoralism and millet cultivation was promoted by the occurrence of drought events during 4,200–4,000 cal yr BP. Moreover, we suggest that mixed rainfed agriculture and animal husbandry increased the adaptability and resilience of the inhabitants of the region which enabled them to occupy the relatively arid environment of the monsoon marginal area of northern China.
Analysis on the Characteristics of Crustal Structure and Seismotectonic Environment in Zigui Basin, Three Gorges
Authors: Wu Yunlong , Pei Junling , Wang Ze , Zhang Yi , Yuan Huaqing
Published: November 2021

The Zigui Basin in the Three Gorges area is a syncline running in the north-south axial direction; the sediments in the basin are mainly late Triassic-late Jurassic sandy and argillaceous rocks. Since the Three Gorges Project began undergoing impoundment in May 2003, nearly 20,000 mini-earthquakes have occurred in the Zigui–Badong region, including five earthquakes with magnitudes greater than Ms 5.0. Herein, a 3D gravity inversion method was introduced to investigate the density structure of the Zigui Basin and its adjacent areas. A comprehensive analysis of the geological structure of the area was carried out, based on focal mechanism solutions of six moderate–strong earthquakes in the area. The Zigui Basin is a low–density area from the periphery, and the sediment in the east is thicker but less dense than in the west. The shallow part of the Zigui Basin is a weak bottom layer prone to slippage, and the deep part (5–10 km) could comprise lithological strata such as limestone, sandstone, and shale, which easily undergo dissolution by leaking reservoir water. Under the action of external forces such as long-term infiltration and unloading of reservoir water, fissures will expand and squeeze then earthquakes are likely induced in the deep strata. Furthermore, new buried faults in the western and southeastern edges of the core area of the Zigui Basin could also trigger an earthquake under long–term pressurization and reservoir water penetration.
Disturbance Process of Sandy Gravel Stratum Caused by Shield Tunneling and Ground Settlement Analysis
Authors: Qingke Nie , Guang Sun , Siyuan Gao , Hongtao Liu , Lichao Zhou , Jianpeng Hou
Published: November 2021

This paper analyzed the earth pressure, pore pressure, and surface settlement of the Luoyang urban rail transit tunnel in a sandy gravel stratum (Henan Province, China) under different burial depths by using field measurement methods. The results showed that the earth pressure as well as pore pressure of the soil layer above the working surface increased sharply and reached their maximum values when the cutter head of the shield gradually crossed the working surface. During the completion of synchronous grouting, the earth pressure and pore pressure increased slightly; when shield tunneling passed through the working surface, the earth pressure is smaller than the original earth pressure due to the unloading effect. The surface settlement curve above the tunnel took on a “V” shape after the completion of the left-side tunnel excavation, conforming to the normal distribution pattern. The surface settlement curve above the two tunnels took on a “W” shape after the completion of the right-side tunnel excavation, which is in good agreement with the proposed theoretical calculations. The findings of this study can help for better understanding the control of safety risk during shield construction.
Lattice Numerical Simulations of Hydraulic Fracture Propagation and Their Geometry Evolution in Transversely Isotropic Formations
Authors: Qiu Dezhi , Zhang Jun , Lin Yinhe , Liu Jinchuan , Rabiei Minou , Rasouli Vamegh , Damjanac Branko , Huang Rui
Published: November 2021

Accurate prediction of the fracture geometry before the operation of a hydraulic fracture (HF) job is important for the treatment design. Simplified planar fracture models, which may be applicable to predict the fracture geometry in homogeneous and continuous formations, fail in case of fractured reservoirs and laminated formations such as shales. To gain a better understanding of the fracture propagation mechanism in laminated formations and their vertical geometry to be specific, a series of numerical models were run using XSite, a lattice-based simulator. The results were studied to understand the impact of the mechanical properties of caprock and injection parameters on HF propagation. The tensile and shear stimulated areas were used to determine the ability of HF to propagate vertically and horizontally. The results indicated that larger caprock Young’s modulus increases the stimulated area (SA) in both vertical and horizontal directions, whereas it reduces the fracture aperture. Also, larger vertical stress anisotropy and tensile strength of caprock and natural interfaces inhibit the horizontal fracture propagation with an inconsiderable effect in vertical propagation, which collectively reduces the total SA. It was also observed that an increased fluid injection rate suppresses vertical fracture propagation with an insignificant effect on horizontal propagation. The dimensionless parameters defined in this study were used to characterize the transition of HF propagation behavior between horizontal and vertical HFs.
Embodied Instruction: An Exploration and Qualitative Comparison of Expression and Performance of Yoga Instruction
Authors: Reeves Tegan , White Crystal L.
Published: November 2021

Background: An inclusive, whole-child model of education is shifting the paradigm into an integrative mind-body approach. Toward contextualizing learning at the close of the cartesian era, the current work explores embodied instruction through the lens of a teacher whose primary focus is the integration of mind and body. Perhaps the longest standing curriculum aimed at embodied learning, yoga provides a unique perspective on techniques and experiences of embodied teaching.

Method: This case study employed an in-depth explorative participatory design to observe instructor intentions in and performance of instruction. Consecutive interviews (5) in conjunction with participatory observation of weekly classes (8) were audio recorded, transcribed verbatim, and triangulated with researcher memos. A grounded and comparative methodology was used to analyze expressed understanding of embodied learning and performed instruction.

Results: In-depth interviews revealed four themes of expressed understanding of embodied instruction: energetic state, personality of instruction, inviting experience and student vulnerability. Participatory observations revealed four themes of performance of embodied instruction: scoping, cadence, silence, and inviting practice. Overlap in expressed understanding and observed instruction were found in each theme. An example of the expressed understanding of a thematic concept is inviting experience: “If someone comes in the very first time and the teacher is very invasive and says, “Do this, do this, do this.” And the next pose the teacher is hovering over them, you are not going to go back. You are going to hate it so much [I] let people be really all over the place at the beginning as long as they are safe. [I] allow them that kind of freedom, initially”. This was further supported in the observed instruction; an example of this is: “We’re going to play with [a posture]. that firmness in your abs, engage the core so a foot might lift. Maybe both feet.” And “with the arms firm, maybe the legs straighten”.

Conclusion: The current study yields preliminary insight into yoga instruction strategies to support further development of embodied teaching and learning.
Flavonoids: Classification, Function, and Molecular Mechanisms Involved in Bone Remodelling
Authors: Ramesh Priyanka , Jagadeesan Rahul , Sekaran Saravanan , Dhanasekaran Anuradha , Vimalraj Selvaraj
Published: November 2021

Flavonoids are polyphenolic compounds spotted in various fruits, vegetables, barks, tea plants, and stems and many more natural commodities. They have a multitude of applications through their anti-inflammatory, anti-oxidative, anti-carcinogenic properties, along with the ability to assist in the stimulation of bone formation. Bone, a rigid connective body tissue made up of cells embedded in a mineralised matrix is maintained by an assemblage of pathways assisting osteoblastogenesis and osteoclastogenesis. These have a significant impact on a plethora of bone diseases. The homeostasis between osteoblast and osteoclast formation decides the integrity and structure of the bone. The flavonoids discussed here are quercetin, kaempferol, icariin, myricetin, naringin, daidzein, luteolin, genistein, hesperidin, apigenin and several other flavonoids. The effects these flavonoids have on the mitogen activated protein kinase (MAPK), nuclear factor kappa β (NF-kβ), Wnt/β-catenin and bone morphogenetic protein 2/SMAD (BMP2/SMAD) signalling pathways, and apoptotic pathways lead to impacts on bone remodelling. In addition, these polyphenols regulate angiogenesis, decrease the levels of inflammatory cytokines and play a crucial role in scavenging reactive oxygen species (ROS). Considering these important effects of flavonoids, they may be regarded as a promising agent in treating bone-related ailments in the future.
Study of a High Temperature–Resistant Shielding Material for the Shielding Doors of Nuclear Power Plants
Authors: Xiao-ling LI , Rong-jun Wu , Xiao-hui Xu , Duo-fei Zhang , Ming YU
Published: November 2021

An optimization design and application of high temperature–resistant shielding material was carried out according to the nuclear power plant source characteristics and special protection requirements such as loss-of-coolant accident (LOCA). The composition of lead–boron polyethylene shielding composite was optimized based on the genetic algorithm and Monte Carlo methods and then realized by blending modification and graft copolymerization to improve its high temperature–resistant, shielding, and mechanical properties. Then comprehensive properties such as mechanical, neutron shielding, damp heat aging, irradiation resistance, and high temperature resistance were tested. These experiments proved that the high temperature–resistant lead–boron polyethylene shielding composite has excellent performance; especially, as it is able to keep a complete structure in a high-temperature environment of up to 190°C for 48 h. Finally, the shielding composite was applied to the shielding door design of a reactor pit chamber. When the shield thickness is 60 mm, the level of the neutron dose rate was reduced by 10 times, and that of the γ dose rate was reduced by 5 times, which meets all the requirements of radiation protection safety for nuclear power plants.
Will Low-Carbon Purchasing Behavior Make Residents’ Behaviors Greener? Research Based on Spillover Effects
Authors: Yue Ting , Zhang Lan , Long Ruyin , Chen Hong , Gao Chenchen , Li Mengting
Published: November 2021

Low-carbon purchasing behavior is the core part of low-carbon consumption behavior. Its impact on low-carbon use behavior, recycling behavior, and garbage sorting behavior needs to be further clarified. Based on self-perception theory and cognitive dissonance theory, this paper constructs a theoretical model of the spillover effect of low-carbon purchasing behavior on low-carbon use behavior, recycling behavior and garbage sorting behavior through self-efficacy and environmental self-identity. This paper uses the Bootstrap method to analyze 494 valid questionnaires empirically. The results show that: low-carbon purchasing behavior has a significantly positive effect on low-carbon use behavior, recycling behavior, and garbage sorting behavior. Self-efficacy and environmental self-identity play partially parallel mediating roles in the spillover effect. The mediating effect of environmental self-efficacy is stronger than environmental self-identity between low-carbon purchasing behavior and low-carbon use behavior. The mediating effect of self-efficacy is weaker than environmental self-identity between low-carbon purchasing behavior and recycling behavior or garbage sorting behavior. The spillover effects are different in demographic variables. The spillover effect of men is more substantial than women. The spillover effect of residents in 18–44 years old is more significant than residents in other age groups. The spillover effect of married residents is the largest. Residents with a bachelor’s degree have the most significant spillover effects on recycling behavior and garbage sorting behavior than residents with other educational levels. Professional and technical personnel have the most considerable spillover effect than other occupation types. The spillover effect is the largest when the family have three members. The spillover effect of residents with a monthly household income of 6,000–8,000 yuan is the largest between low-carbon purchasing behavior and low-carbon use behavior.
Enhanced Enzymatic Hydrolysis of Cellulose From Substrate and Indole-3-Acetic Acid Content—During the Fruiting Body Differentiation Stage by Sodium Acetate Addition
Authors: Hou Li-juan , Li Zheng-peng , Li Chang-tian , Lin Jin-sheng , Ma Lin , Jiang Ning , Qu Shao-xuan , Li Hui-ping , Li Yu
Published: November 2021

Volvariella volvacea, with high commercial, nutritional and medicinal value, is widely cultivated in tropical and subtropical regions. The effects of supplementation on mushroom yield has been studied. We showed that the optimal application of sodium acetate (NaAc) was spray application of a 0.08% concentration during the substrate mixing stage which could increase yields by up to 89.16% and enhance the enzymatic hydrolysis of cellulose and hemicellulose from the substrate. For most enzymes tested maximum activity occurred during the fruiting body growth and development stage, which led to degradation of the substrate, increasing the available nutrients for mycelial propagation and fruiting body growth and development. Meanwhile, NaAc also significantly increased the indole-3-acetic acid (IAA) content in the early fruiting body development stage of V. volvacea, It was observed that IAA promotes not only plant primordium differentiation; but also the primordium differentiation of edible fungi. Furthermore, treatments with three acetate salts had an increase of yield by 30.22% on average. The mechanisms by which NaAc application may improve the yield of V. volvacea are discussed.
Univariable and Multivariable Two-Sample Mendelian Randomization Investigating the Effects of Leisure Sedentary Behaviors on the Risk of Lung Cancer
Authors: Peng Haoxin , Wu Xiangrong , Wen Yaokai , Ao Yiyuan , Li Yutian , Guan Wenhui , Lin Jinsheng , Li Caichen , Liang Hengrui , He Jianxing , Liang Wenhua
Published: November 2021

Background:

Leisure sedentary behaviors (LSB) are widespread, and observational studies have provided emerging evidence that LSB play a role in the development of lung cancer (LC). However, the causal inference between LSB and LC remains unknown.

Methods: We utilized univariable (UVMR) and multivariable two-sample Mendelian randomization (MVMR) analysis to disentangle the effects of LSB on the risk of LC. MR analysis was conducted with genetic variants from genome-wide association studies of LSB (408,815 persons from UK Biobank), containing 152 single-nucleotide polymorphisms (SNPs) for television (TV) watching, 37 SNPs for computer use, and four SNPs for driving, and LC from the International Lung Cancer Consortium (11,348 cases and 15,861 controls). Multiple sensitivity analyses were further performed to verify the causality.

Results: UVMR demonstrated that genetically predisposed 1.5-h increase in LSB spent on watching TV increased the odds of LC by 90% [odds ratio (OR), 1.90; 95% confidence interval (CI), 1.44–2.50; p < 0.001]. Similar trends were observed for squamous cell lung cancer (OR, 1.97; 95%CI, 1.31–2.94; p = 0.0010) and lung adenocarcinoma (OR, 1.64; 95%CI 1.12–2.39; p = 0.0110). The causal effects remained significant after adjusting for education (OR, 1.97; 95%CI, 1.44–2.68; p < 0.001) and body mass index (OR, 1.86; 95%CI, 1.36–2.54; p < 0.001) through MVMR approach. No association was found between prolonged LSB spent on computer use and driving and LC risk. Genetically predisposed prolonged LSB was additionally correlated with smoking (OR, 1.557; 95%CI, 1.287–1.884; p < 0.001) and alcohol consumption (OR, 1.010; 95%CI, 1.004–1.016; p = 0.0016). Consistency of results across complementary sensitivity MR methods further strengthened the causality.

Conclusion: Robust evidence was demonstrated for an independent, causal effect of LSB spent on watching TV in increasing the risk of LC. Further work is necessary to investigate the potential mechanisms.
Two-Component System Genes in Sorghum bicolor: Genome-Wide Identification and Expression Profiling in Response to Environmental Stresses
Authors: Zameer Roshan , Sadaqat Muhammad , Fatima Kinza , Fiaz Sajid , Rasul Sumaira , Zafar Hadeqa , Qayyum Abdul , Nashat Naima , Raza Ali , Shah Adnan Noor , Batool Riffat , Azeem Farrukh , Sun Sangmi , Chung Gyuhwa
Published: November 2021

The two-component signal transduction system (TCS) acts in a variety of physiological processes in lower organisms and has emerged as a key signaling system in both prokaryotes and eukaryotes, including plants. TCS genes assist plants in processes such as stress resistance, cell division, nutrition signaling, leaf senescence, and chloroplast division. In plants, this system is composed of three types of proteins: response regulators (RRs), histidine kinases (HKs), and histidine phosphotransfer proteins (HPs). We aimed to study the Sorghum bicolor genome and identified 37 SbTCS genes consisting of 13 HKs, 5 HPs, and 19 RRs (3 type-A RRs, 7 type-B RRs, 2 type-C RRs, and 7 pseudo-RRs). The structural and phylogenetic comparison of the SbTCS members with their counterparts in Arabidopsis thaliana, Oryza sativa, Cicer arietinum, and Glycine max showed group-specific conservations and variations. Expansion of the gene family members is mostly a result of gene duplication, of both the tandem and segmental types. HKs and RRs were observed to be originated from segmental duplication, while some HPs originated from tandem duplication. The nuclear genome of S. bicolor contain 10 chromosomes and these SbTCS genes are randomly distributed on all the chromosomes. The promoter sequences of the SbTCS genes contain several abiotic stress-related cis-elements. RNA-seq and qRT-PCR-based expression analysis demonstrated most of the TCS genes were responsive to drought and salt stresses in leaves, which suggest their role in leaf development. This study lays a foundation for further functional study of TCS genes for stress tolerance and developmental improvement in S. bicolor.
Prediction of Hormone-Binding Proteins Based on K-mer Feature Representation and Naive Bayes
Authors: Guo Yuxin , Hou Liping , Zhu Wen , Wang Peng
Published: November 2021

Hormone binding protein (HBP) is a soluble carrier protein that interacts selectively with different types of hormones and has various effects on the body’s life activities. HBPs play an important role in the growth process of organisms, but their specific role is still unclear. Therefore, correctly identifying HBPs is the first step towards understanding and studying their biological function. However, due to their high cost and long experimental period, it is difficult for traditional biochemical experiments to correctly identify HBPs from an increasing number of proteins, so the real characterization of HBPs has become a challenging task for researchers. To measure the effectiveness of HBPs, an accurate and reliable prediction model for their identification is desirable. In this paper, we construct the prediction model HBP_NB. First, HBPs data were collected from the UniProt database, and a dataset was established. Then, based on the established high-quality dataset, the k-mer (K = 3) feature representation method was used to extract features. Second, the feature selection algorithm was used to reduce the dimensionality of the extracted features and select the appropriate optimal feature set. Finally, the selected features are input into Naive Bayes to construct the prediction model, and the model is evaluated by using 10-fold cross-validation. The final results were 95.45% accuracy, 94.17% sensitivity and 96.73% specificity. These results indicate that our model is feasible and effective.
Easing of Regulatory Barriers to Telemedicine Abortion in Response to COVID-19
Authors: Skuster Patty , Dhillon Jina , Li Jessica
Published: November 2021

For many people seeking abortion during the continuing COVID-19 pandemic, telemedicine abortion is the safest and most acceptable method, posing lower risk of exposure to the virus. In addition, by reducing in-person visits with health care providers, increased use of telemedicine for abortion can reduce pressure on overburdened health systems. Given the benefits of telemedicine during the pandemic, government agencies in several countries took measures to temporarily allow telemedicine abortion. We conducted key-word English-language searches to identify examples of government action to remove regulatory barriers to the practice of telemedicine abortion in response to the pandemic. We found instances of government agencies in eight countries taking steps to ease regulatory barriers to telemedicine abortion. Telemedicine abortion is safe, cost-effective, and may be the preferred method of abortion during acute periods of COVID-19 transmission, as well as after the pandemic has abated. As one step to expanding access to abortion with medicine where abortion is legal, health agencies and other regulatory bodies can take steps to remove barriers specific to telemedicine abortion.
Six Autoimmune Disorders Are Associated With Increased Incidence of Gastric Cancer: A Systematic Review and Meta-Analysis of Half a Million Patients
Authors: Zádori Noémi , Szakó Lajos , Váncsa Szilárd , Vörhendi Nóra , Oštarijaš Eduard , Kiss Szabolcs , Frim Levente , Hegyi Péter , Czimmer József
Published: November 2021

Background

Gastric cancer is one of the most common cancers worldwide, with a high mortality rate. The potential etiological role of autoimmune (AI) disorders has been described in gastric cancer; however, the literature is controversial. This study aims to provide a comprehensive summary of the association between autoimmune disorders and the incidence of gastric cancer.

Methods

This study was registered on PROSPERO under registration number CRD42021262875. The systematic literature search was conducted in four scientific databases up to May 17, 2021. Studies that reported standardized incidence rate (SIR) of gastric cancer in autoimmune disorders were eligible. We calculated pooled SIRs with 95% confidence intervals (CIs) in this meta-analysis.

Results

We included 43 articles describing 36 AI disorders with data of 499,427 patients from four continents in our systematic review and meta-analysis. Significantly increased incidence of gastric cancer was observed in dermatomyositis (SIR = 3.71; CI: 2.04, 6.75), pernicious anemia (SIR = 3.28; CI: 2.71, 3.96), inflammatory myopathies (SIR = 2.68; CI:1.40; 5.12), systemic lupus erythematosus (SIR = 1.48; CI: 1.09, 2.01), diabetes mellitus type I (SIR = 1.29; CI:1.14, 1,47), and Graves’ disease (SIR = 1.28; CI: 1.16, 1.41). No significant associations could be found regarding other AI disorders.

Conclusions

Pernicious anemia, Graves’ disease, dermatomyositis, diabetes mellitus type I, inflammatory myopathies, and systemic lupus erythematosus are associated with higher incidence rates of gastric cancer. Therefore, close gastroenterological follow-up or routinely performed gastroscopy and application of other diagnostic measures may be cost-effective and clinically helpful for patients diagnosed with these autoimmune diseases.
S100A2 Is a Prognostic Biomarker Involved in Immune Infiltration and Predict Immunotherapy Response in Pancreatic Cancer
Authors: Chen Yuan , Wang Chengcheng , Song Jianlu , Xu Ruiyuan , Ruze Rexiati , Zhao Yupei
Published: November 2021

Pancreatic cancer (PC) is a highly fatal and aggressive disease with its incidence and mortality quite discouraging. It is of great significance to construct an effective prognostic signature of PC and find the novel biomarker for the optimization of the clinical decision-making. Due to the crucial role of immunity in tumor development, a prognostic model based on nine immune-related genes was constructed, which was proved to be effective in The Cancer Genome Atlas (TCGA) training set, TCGA testing set, TCGA entire set, GSE78229 set, and GSE62452 set. Furthermore, S100A2 (S100 Calcium Binding Protein A2) was identified as the gene occupying the most paramount position in risk model. Gene set enrichment analysis (GSEA), ESTIMATE and CIBERSORT algorithm revealed that S100A2 was closely associated with the immune status in PC microenvironment, mainly related to lower proportion of CD8+T cells and activated NK cells and higher proportion of M0 macrophages. Meanwhile, patients with high S100A2 expression might get more benefit from immunotherapy according to immunophenoscore algorithm. Afterwards, our independent cohort was also used to demonstrate S100A2 was an unfavorable marker of PC, as well as its remarkably positive correlation with the expression of PD-L1. In conclusion, our results demonstrate S100A2 might be responsible for the preservation of immune-suppressive status in PC microenvironment, which was identified with significant potentiality in predicting prognosis and immunotherapy response in PC patients.
Engineered CAR-Macrophages as Adoptive Immunotherapies for Solid Tumors
Authors: Sloas Christopher , Gill Saar , Klichinsky Michael
Published: November 2021

Cellular immunotherapies represent a promising approach for the treatment of cancer. Engineered adoptive cell therapies redirect and augment a leukocyte’s inherent ability to mount an immune response by introducing novel anti-tumor capabilities and targeting moieties. A prominent example of this approach is the use of T cells engineered to express chimeric antigen receptors (CARs), which have demonstrated significant efficacy against some hematologic malignancies. Despite increasingly sophisticated strategies to harness immune cell function, efficacy against solid tumors has remained elusive for adoptive cell therapies. Amongst cell types used in immunotherapies, however, macrophages have recently emerged as prominent candidates for the treatment of solid tumors. In this review, we discuss the use of monocytes and macrophages as adoptive cell therapies. Macrophages are innate immune cells that are intrinsically equipped with broad therapeutic effector functions, including active trafficking to tumor sites, direct tumor phagocytosis, activation of the tumor microenvironment and professional antigen presentation. We focus on engineering strategies for manipulating macrophages, with a specific focus on CAR macrophages (CAR-M). We highlight CAR design for macrophages, the production of CAR-M for adoptive cell transfer, and clinical considerations for their use in treating solid malignancies. We then outline recent progress and results in applying CAR-M as immunotherapies. The recent development of engineered macrophage-based therapies holds promise as a key weapon in the immune cell therapy armamentarium.