bluuebunny/crossref_metadata_2025 huggingface dataset:
Transposon insertion sequencing (Tn-seq) library preparation protocol - includes UMI for PCR duplicate removal v1
Authors: Palani Nagendra
Published: January 2019

Transposon insertion sequencing (Tn-Seq) is an NGS method to en masse map genomic locations of transposon insertions in a pool of mutants. Insertion mutants are usually made using synthetic mini transposons like Tn5 and Himar (in bacteria), Sleeping Beauty & Piggybac (in mammalian cells), and others like Mos (in Drosophila / C. elegans). There are several NGS methods to perform de novo Tn-Seq mapping of a library of mutants. Some of the key ones are listed below. Van Opijnen, Tim, Kip L. Bodi, and Andrew Camilli. "Tn-seq: high-throughput parallel sequencing for fitness and genetic interaction studies in microorganisms."Nature methods 6.10 (2009): 767. - Uses MmeI (a type IIs restriction enzyme) digestion to generate ~ 18bp genomic DNA overhangs from Mariner transposon insertions. Adapters are ligated to the fragments, the transposon - genome junction is enriched by PCR before being sequenced. Because of the short genomic sequence available for alignment to reference, mapping accuracy can be lower compared to fragmentation based library prep methods. Langridge, Gemma C., et al. "Simultaneous assay of every Salmonella Typhi gene using one million transposon mutants."Genome research (2009). - DNA extracted from a pool of mutants is sheared/fragmented to a desired fragment size (usually 300 - 400 bp), fragments are end-repaired, and the transposon - genome junction is enriched by PCR before being sequenced. The list is only intended to highlight two major approaches to Tn-seq library prep - restriction digestion based or fragmentation based. There are several variations on each approach that are now available for the discerning scientist. There are also less known but interesting alternative methods like HTML-PCR. After trying out several of the above methods, we have developed a Tn-Seq library preparation protocol that incorporates desirable features from existing protocols and new features that will improve data quality, while being easy to perform (an undergraduate student with some wet-lab experience can go through the protocol in a day). The protocol is conceptually similar to the TraDIS protocol (see the Langridge et al paper above). Key features are: 1. Uses the NEB Ultra II FS kit for enzymatic fragmentation of genomic DNA, end-repair of fragments, and ligation of adaptor. The kit allows performing all the reactions sequentially in a single tube (one-pot prep). 2. Incorporates Unique Molecular Identifiers (UMI) to enable removing PCR duplicates from sequencing data. Improves read count accuracy when the data is to be used for read-frequency based analyses (fitness or log fold change). 3. Includes 'best practices' library features like color diversity phasing and separate indexing step to save cost on adaptor oligos. The method is also automation friendly.
Dynamische Erweiterung von eCl@ss
Authors: Nagel Jörg , Bondza Artur , Dogan Alaettin , Hildebrandt Constantin , Höpfner Andreas , Kehl Tobias , Rauscher Benedikt , Gehlen Stefan , Eck Christian , Fay Alexander
Published: January 2019

Von den Autoren wurde ein neuer Ansatz entwickelt und implementiert, der Entwicklern ermöglicht, Anwendungen, die im Zuge der fortschreitenden Digitalisierung auf der Analyse und Kombination von Daten basieren, unmittelbar bei Erstellung mit eCl@ss-Merkmalen zu verknüpfen und bei Bedarf neuen eCl@ss-Content hinzuzufügen. Die Datenmodelle der Applikationen können damit vollständig und lückenlos bereits in der Entwicklung auf Basis von eCl@ss-Merkmalen erstellt und die Datenentstehung vollständig von der Datennutzung getrennt werden. Neuer Content wird mit Hilfe des Ansatzes sofort verfügbar und die Daten der Applikation können durch Dritte interpretiert werden. Mittels eines auf Industriestandards basierenden Informationsmodells gelingt die Sicherung der Qualität neu hinzugefügter Merkmale. Die damit verbundene Erleichterung der Qualitätsprüfung neuen eCl@ss-Contents soll zu einer flexibleren und schnelleren Nutzung des Klassifikationssystems für neue Anwendungen führen. Der vorgeschlagene Ansatz zeigt einen Weg auf, wie Klassifikationssysteme wie eCl@ss agil weiterentwickelt werden können und mit der Dynamik der Entwicklung neuer Anwendungen schritthalten.
Von der Laboranalyse zum Feldgerät
Authors: Falk Judith , Heisterkamp Monika , Kaiser Ulrich
Published: January 2019

Die Prozessanalytik gibt Aufschluss über die stoffliche Zusammensetzung von Medien in verfahrenstechnischen Prozessen und bildet die entscheidende Basis für eine Optimierung dieser Prozesse. Wünschenswert ist dabei, die analytische Messtechnik prozessnah als Feldgerät verfügbar zu haben. Dieser Beitrag beschreibt relevante Verfahren der Prozessanalytik und zeigt mit welchen Methoden und Technologien Analysenmesstechnik als prozessnahe Feldgeräte implementiert werden kann.
PREVALENCE OF BETA THALASSEMIA IN A SAMPLE OF SCHOOL AGE CHILDREN IN SULAIMANI CITY
Authors: Mustafa Ahmed , Hassan Adnan
Published: January 2019

Background 

Beta thalassemia is a major health problem in Sulaimani. The disease is usually diagnosed early but puts a huge burden on the health sector due, as well as a high morbidity and mortality of the Patient. The carrier state is mostly under diagnosed.

Objectives 

To determine the prevalence of Beta thalassemia trait in school aged children in Sulaimani city.

Materials and Methods

A prospective cross-sectional study, undertook in 2 months peroid in 2017 in Sulaimani city, in which a total of 300 students were randomly taken from five primary school. A complete blood count was done, the serum hemoglobin electrophoresis and iron studies were done for individuals with anemia (PCV < 36%) or microcytosis (MCV < 76 fL).

Results

Out of 300 subjects 43 (14.3%) had anemia (including 17 (5.7%) had iron deficiency anemia), 13 (4.3%) had Beta thalassemia (major and minor).

Conclusion

The high prevalence rate of Beta thalassemia carrier state necessitates strengthening the preventive program, and neonatal screening to control and prevent cases of thalassemia major.
HISTOPATHOLOGICAL CHARACTERISTICS OF GASTRIC CARCINOMA AND ITS RELATION WITH ABO BLOOD GROUP IN KURDISH PEOPLE
Authors: Daood Rawaz , Salaye Jangy
Published: January 2019

Background 

Many studies have been done of relationship between blood group and cancers. 

Objectives 

To evaluate the relationship between ABO blood group system and the risk of gastric cancer with its histopathological features among Kurdish population by case-control study.

Materials and Methods

In our retrospective case-control study, 194 cases of gastric cancer were enrolled from March 2013 to October 2017 The patients were of Kurdish ethnicity, data on age, sex, hisopathological result, blood group, grade and stage at diagnosis were collected, association with ABO blood group and gastric cancer were assessed.

Results

This study showed that blood group A might be a risk factor of gastric cancer in both males and females. The intestinal type adenocarcinomas were the most frequent hisopathological subtype of gastric carcinoma among blood group A. Grade 3 of differentiation found to be most frequent in blood group A patients (20.1%).

Conclusion

Individuals with blood group A has a higher risk of developing gastric cancer in their lifetime and their disease would be of high grade which might predict poor prognosis, while people with blood group AB have the lowest risk of developing gastric cancer.
16 Nisan 2017 Anayasa Değişikliği Referandumu Sürecinde Siyasal Bilgilenme ve Medya: Eğitim Düzeyine Göre Bir Karşılaştırma
Authors: BALCI Şükrü
Published: January 2019

Birey siyasal bilgilenme sürecinde, çeşitli faktörlerin etkisinde kalmaktadır.
Günümüzde geleneksel medya araçlarının yanında yeni iletişim teknolojilerinin
gelişimiyle bu süreç daha da çeşitlenmiştir. Söz konusu süreçte bireyler bir takım
demografik özelliklerine göre farklı düzeylerde etkilenmektedir. Bu demografik
özelliklerden biri de bireyin eğitim düzeyidir. Bu çalışmada da Konya’nın Selçuklu,
Karatay ve Meram ilçelerinde ikamet eden insanların eğitim durumlarına göre siyasal
bilgilenme süreçlerinde iletişim araç ve yöntemlerinin etkinliği açısından farklılıkların
ortaya çıkarılması amaçlanmıştır. Saha araştırması yönteminin kullanıldığı bu
çalışmada 600 kişiyle yüz yüze anket tekniği kullanılarak görüşülmüştür. Araştırma
bulgularına göre katılımcıların siyasal bilgilenme sürecinde en çok etkilendiği medya
türleri arasında televizyon ve internet ilk sıralarda yer almaktadır. Araştırma katılan
seçmenlerin siyasal bilgilenme sürecinde interneti etkili görme eğilimi arttıkça,
sosyal medyayı etkili görme eğiliminde de bir artış yaşanmaktadır. Yine eğitim düzeyi
artışı, siyasal bilgilenmede gazete ve internetin etkili olduğunu düşünme yönündeki
eğilimleri de artırmaktadır.
Film Anlatısında Kahramanın Değişen Özne Konumu: Başkalarının Hayatı Filmi Örneği
Authors: MEDİN Burak
Published: January 2019

Geçmişten günümüze sinemasal anlatılar, sundukları öykünün taşıyıcısı olan
kahramanlar/özneler aracılığıyla geniş kitlelere ulaşır. İzler kitlenin ilgisi ve dikkati,
inşa edilen kahraman konumundaki özneler aracılığıyla çekilir. İzleyici, anlatıda
önemli bir eyleyen olan kahramandan hareketle özdeşlik ilişkisini başlatır. Klasik,
çağdaş ya da postmodern anlatılarda kahramanın inşasıyla ilgili durum farklılık
gösterse de yine de anlatı içinde aranan en önemli eyleyen, öznedir. Bu eksende
kahramanların özne konumları açısından irdelenmesi sinemasal dünyayı/yolculuğu
anlamlandırabilmek adına önem taşır. Bu bağlamda bu çalışmada özne konumundaki
kahramana ve öznenin izlencesini kuran, başlatan ve ileriye doğru devindiren yitik/
arzu nesnesine odaklanıldı. Özneyi özdeşlik kurulabilecek ideal egoya/doğruluk
öznesine dönüştüren unsurlar da bu çalışmada ele alınan bir diğer önemli başlıktır.
Değişen özne konumunu anlayabilmede önemli potansiyeller taşıdığı varsayılan
Başkalarının Hayatı (The Lives of Others) filmi bu çalışmanın araştırma nesnesidir.
Araştırma nesnesi konumunda olan bu film anlatısı özne konumu açısından A. J.
Greimas’ın “Eyleyensel Örnekçe” ve “Dörtlü Şema” kuramı bağlamında çözümlendi.
Benlik; kahramanın izlencesini, değişimini ve dönüşümünü anlamak adına önem
taşıyan bir başka unsurdur. Bu bağlamda göstergebilimsel yaklaşımın yanı sıra E.
Goffman’ın “Dramaturjik İlkeleri”nden ve bu ilkelerden hareketle ortaya koyduğu
mikro-sosyolojik kavramlarından da çözümleme sırasında yararlanıldı.
Should you be putting all your eggs into one basket? A look into the current state and future of ovarian tissue cryopreservation.
Authors: Rauff Shakina , Khan Zaraq , Andersen Claus Yding
Published: January 2019

Ã‚Â Ovarian tissue cryopreservation has its primary goal in fertility preservation for women diagnosed with a malignancy and who may be rendered infertile because of the potential gonadotoxic chemotherapy and/or radiotherapy involved in treating their disease. Unlike the standard and endorsed methods of fertility preservation like mature oocyte and embryo cryopreservation, ovarian tissue freezing not only conserves the reproductive capacity of the woman but additionally, maintains the steroidogenic competence of the ovary due to the fact that the frozen cortex contains numerous ovarian follicles Ã¢â‚¬â€œ the functional unit of the ovary. Not every follicle is fated to aid procreation. In fact more than 99% are destined to end up in atresia, which may be viewed as an enormous waste of inherent resources. In light of this, there have been propositions to expand the scope of ovarian tissue cryopreservation and transplantation beyond its traditional purpose of fertility preservation for medical indications. Some of these ideas include utilizing cryopreserved ovarian tissue for induction of puberty, delaying the menopause and fertility preservation for social motives. Needless to say, these novel ideas will evoke questions, controversy and a plethora of criticism about the safety, superiority, cost-effectiveness, implications and necessity of these different utilities. In this article, we aim to explore some of the issues that shroud these new indications and discuss the advantages for and diatribe against these evolving suggestions.
THE LABORATORY STUDY OF THE FLEXURAL STRENGTH OF REINFORCED BIS-ACRYLIC MATERIAL FOR PROVISIONAL FIXED RESTORATIONS
Authors: Петрикас Олег , Petrikas Oleg , Трапезников Дмитрий , TRAPEZNIKOV Dmitry , Змеева Эмилия , Zmeeva Emilia
Published: January 2019

Background. The use of provisional (interim, temporary) restorations has become a routine procedure in modern dentists after the end of the era of brazed-stamped bridges. The use of traditional acrylic plastics does not provide the necessary strength for the long-term functioning of provisional prostheses. Bis-acrylic materials have helped to eliminate some of the problems associated with traditional acrylic materials. However the disadvantage of bis-acryls is that they can break relatively easily when placed in areas of increased stress. The use of provisional prostheses, obtained by casting or CAD/CAM technology, undoubtedly solve problems, however, prosthetics are significantly more expensive. Another known and cheaper way is to harden polymers by reinforcing them. Objectives ― to study, when conducting a mechanical test, the fracture resistance of glass fiber-reinforced bis-acrylic composite beams. Methods. On a universal testing machine (three-point flexural test), 8 groups of samples were studied depending on the material — Re-fine Bright acrylic material (Yamahachi Dental MFG., CO., Japan), or Luxatemp bis-acrylic material (DMG) and Protemp 4 (3M ESPE), and also, the method of reinforcement of Protemp 4 with GlasSpan fiberglass tape (GlasSpan). The fracture strength (F) was calculated in MPa. Statistical differences between groups were determined using T-test. Results. Comparison of the fracture strengths results between the first control group (Protemp 4 bis-acrylic plastic without reinforcement) and other groups (glass tape reinforcement) revealed a significant hardening of Protemp 4 after reinforcement (p <0,05). Conclusions. The use of reinforcing glass tape with a full impregnation with an adhesive and a flowable composite increases the strength of Protemp 4 more than 2 times.
Insights from Sumerian Mythology: The Myth of Enki And Ninmaḫ and the History of Disability
Authors: KAĞNICI Gökhan
Published: January 2019

Research concerning disability or the history of disability is only recently emerging. It can be stated that disability studies in Assyriology are not yet at the desired level. As far as it is known, one of the most ancient texts, perhaps the first that included vital information about the history of disability in the Ancient Mesopotamia, is a Sumerian creation myth, dubbed Enki and Ninmaḫ. This myth is important for the history of disability since, not only did it refer to certain disability types and illnesses but it also emphasized the social integration of the ill and disabled individuals. Providing proofs that disability was related to societal and cultural contexts, the text refuted the understanding, which prevailed for centuries and one that prevails in part even today, that disability concerned only medicine. In ancient Mesopotamian societies, disabled individuals were neither exterminated due to their disabilities and nor were they comprehensively discriminated against in society, political life and various mechanisms of production. On the contrary, they participated in different levels of the state’s administrative system. Moreover, they did not always perceive congenital disabilities as divine punishment and did not associate it with a catastrophe. Disabilities were perceived as good or bad divine signs about daily life practices of human and thus as an indicator of welfare, abundance, prize, challenge, productivity, and solidarity. Including information about the societal fabric of the disability, Enki and Ninmaḫ has a content that can contribute to the history of disability.
Savaş Yılları Osmanlı Kudüs’ünde Mülkiyet Hakkı (1914-1918)
Authors: KARABULUT Sezen
Published: January 2019

Kudüs şehri birçok din ve mezhebe ait toplulukların bir arada yaşadığı özel bir yerdir. Şehir, tarih boyunca çeşitli devletler tarafından yönetilmiştir. Osmanlı Devleti de dört yüzyıl boyunca şehre hâkim olmuş ve bu süreç içerisinde farklı dinlere ait toplulukların din, eğitim ve sosyo-ekonomik alanlardaki yaşamlarını düzenlemek amacıyla çeşitli kanunnameler hazırlamıştır. Gayrimüslimlerin de şehirde dini-kültürel faaliyetlerini sürdürebilmeleri için birtakım kurumlar inşa etmelerine izin verilmiştir. Ancak ilerleyen yıllarda kapitülasyon anlaşmaları yoluyla imtiyaz elde etmiş olan Avrupa Devletlerinin Ortadoğu coğrafyasında yeni hedefler belirlemeleri ile birlikte şehir mülkiyet mücadelesinin yaşandığı bir alana dönüşmüştür. Osmanlı Devleti, Birinci Dünya Savaşı’nın başlaması ile birlikte kapitülasyonları kaldırarak imtiyazlar yoluyla mülk edinen yabancı kurumları denetleme fırsatı elde etmiştir. Bu çalışmada kapitülasyonların kaldırılma sürecinde yabancıların denetimindeki mülklere yönelik hazırlanan talimatnameleri, bunların yürütülmesi sürecinde oluşturulan komisyonların çalışmalarını ve bu sürecin Kudüs gibi kozmopolit bir şehirdeki uygulamalarını incelemeyi amaçlamaktadır. Bunun için Başbakanlık Osmanlı Arşivi’nde yer alan birincil kaynaklar incelenmiş, çalışma ikincil kaynaklar ile de desteklenmiştir.
MOSCOW REGION ANALYZES ACHIEVEMENTS OF TARGET INDICATORS OF MORTAL REDUCTION IN THE FRAMEWORK OF IMPLEMENTATION OF MAY DECREES OF THE PRESIDENT OF RUSSIA
Authors: Yolkina O. E.
Published: January 2019

The article focuses on the problems of mortality rate and the ways of its reducing in the Moscow Region as well as correct coding of causes of death according to ICD-10.
Тринаест књига из личне библиотеке Јована Стерије Поповића
Authors: Ракић Драгана
Published: January 2019

Јован Стерија Поповић (1806—1856), један од најзначајнијих писаца српске књижевности, просветитељ и научник, поседовао је богату личну библиотеку од које је данас, нажалост, сачувано само 13 књига. Идентификоване су као Стеријине на основу његовог аутографа који се налази на њима или на основу стихова које је својом руком записао. Свих 13 се чувају у Вршцу, 10 припадају Градској библиотеци, а три су у власништву Градског музеја. Претпоставља се да се понека књига са Стеријиним потписом може данас наћи код приватних колекционара.Након Стеријине смрти, његову библиотеку је наследила супруга Јелена. Она је већину књига продала Францу Ветлу, књижару и издавачу из Темишвара, тако да је лична библиотека чувеног писца веома брзо након његове смрти расформирана.
Assentamentos urbanos e autoconstrução: inserção na cidade saudável
Authors: Rosa Adriana Aparecida Carneiro , Victorio Evandra Ramos
Published: January 2019

A acelerada urbanização nos grandes centros muitas vezes não segue diretrizes ideais de crescimento, provocando sérios problemas de degradação ambiental e grandes prejuízos na qualidade de vida da população. Para compreender o espaço urbano dentro da relação dos assentamentos irregulares e da autoconstrução das grandes cidades, precisa estar atento a inúmeros quesitos, dentre eles, o surgimento de favelas que representa falha do poder público. Nas primeiras consequências da ocupação do espaço, percebe-se que devido a acelerada urbanização, cidades sofrem influência de todo complexo da estrutura urbana, principalmente em áreas mais precárias. Contudo, em respeito ao cotidiano das comunidades, bem como a valorização do ponto de vista dos moradores e ativos das favelas, olhares para esses lugares não deveriam vir como um problema, mas sobretudo como um desafio. Acredita-se que o estabelecimento de princípios de desenho urbano que levem em conta “o enxergar” esses moradores como protagonistas, onde existe sim o problema das habitações precárias, entretanto pode-se enfrentar esse repto e então propor soluções para locais bem consolidados, em busca da qualidade de vida, onde o legado é muito maior. Como proposta de intervenção interdisciplinar, esses espaços urbanos podem reduzir o impacto nas áreas mais vulneráveis e, consequentemente, transformar a infraestrutura melhorando o ambiente de forma geral na inserção da cidade saudável.
Cidades saudáveis, estratégias e desafios em novos tempos
Authors: Westphal Marcia Faria
Published: January 2019

Neste artigo apresentamos uma estratégia de desenvolvimento local - Cidades Saudáveis que tem sido praticada em alguns municípios  e regiões do país, desde os anos de 1990.  O objetivo é apresentar um modelo de desenvolvimento local – Cidades Saudáveis que já tem sido assumido por vários municípios do país desde 1990, que associado a novas agendas com as quais o país tem se comprometido, possa ajudar o país a superar a crise política, econômica e de valores que está vivendo, enfrentar o processo de urbanização crescente e superar as crescentes inequidades que tem dificultado a vida da população que ocupa e trabalha nas grandes, médias e pequenas cidades. O movimento por Cidades Saudáveis tem sido fomentado e experimentado no país desde finais da década de 80, com sucesso em algumas regiões do país. O modelo é reapresentado, com todas as suas caraterísticas e aventada a possibilidade de formar redes de municípios, que se ajudem mutuamente e deem força a novos projetos. Novas agendas da Organização das Nações Unidas, como os Objetivos do Desenvolvimento do Milênio, com a qual o Governo Brasileiro esteve comprometido e que foi finalizada com êxito em 2014, envolveu municípios brasileiros como um compromisso local e do país e seus indicadores serviram como base para avaliação de projetos de Cidades Saudáveis e Agenda 21 ao seu final. Agora nova agenda e novo compromisso com os Objetivos do Desenvolvimento Sustentado, foram assumidos pelo país que se comprometeu a atingir metas até 2030. Mais de 200 prefeitos, indicados pela Associação dos Prefeitos, já assinaram um termo de compromisso da sua administração com os Objetivos do Desenvolvimento Sustentado, incluindo-se entre os municípios inseridos no programa de Cidades Sustentáveis. Nesta época de mudanças esse artigo pode ajudar os interessados a encontrar novos apoios teórico práticos para encontrar novos rumos para o desenvolvimento de agendas que indiquem caminhos para diminuir as iniquidades e melhorar a qualidade de vida e saúde da população.
O imaginário do arquivo em divertida mente: o papel dos agentes de memória na construção do ser informacional
Authors: Rangel Thayron Rodrigues , Silva André Januário da
Published: January 2019

Objetiva discutir o contexto das instituições arquivísticas e o papel dos profissionais de arquivo na atualidade. Demonstrar como o paradigma da informação tem redimensionado não apenas as atividades dos arquivistas, mas também os usos e usuários do documento arquivístico. Para tanto, buscamos traçar um paralelo com o filme Divertida Mente (2015) que conta a história de Riley, uma menina de 11 anos, que vive em conflito, consigo e com seus pais, após se mudar de sua cidade natal. O filme é protagonizado pelas emoções, Alegria, Tristeza, Medo, Nojinho e Raiva, responsáveis por conferir à menina suas características de comportamento e também pelo armazenamento e tratamento de suas memórias. Nesse sentido, é possível apreender através do filme a ideia da mente como uma instituição arquivística, e das emoções como profissionais de arquivo responsáveis pela acumulação, seleção, armazenamento e descarte das memórias da personagem. Temos nessa realidade imaginada através do filme, as memórias de Riley como documentos arquivísticos fundamentais na constituição da identidade da personagem e na sua interação com os grupos sociais dos quais faz parte. Assim, podemos dizer que as instituições arquivísticas são ambientes orgânicos, em transformação e fortemente marcados pela atuação dos arquivistas, vistos como agentes de memória fundamentais na formação das identidades. Essa representação vai de encontro a algumas das principais características cristalizadas na área, como a naturalidade dos documentos e a imparcialidade dos profissionais de arquivo. Por fim, também redimensiona o documento arquivístico que passa a ter seu horizonte traçado pela noção de informação.
ANALISIS PROTEKSI KEBAKARAN PADA PERUSAHAAN PRODUKSI GAS DAN PEMBANGKIT LISTRIK
Authors: Romadhon Bery
Published: January 2019

PT. Gresik Gases Indonesia and PT. Gresik Power Indonesia is a company engaged in the production of gas and power plants with natural gas and diesel power. This production site has a potential fire hazard so it must be implemented active and passive fire protection system. This research held to determine the suitability of active fire protection systems, passive in the workplace based on with several standards such as the SNI 03-3985-2000, NFPA 13, Permenaker no. 04/1980, Permen PU no. 26/PRT/M/2008, SNI 03-1745-2000. Observational data collection used regulated checklists, with cross-sectional research design. This production site was the potential for serious hazards to cause large area fire and in a short time. Classification of fire in this production of type A, B, C were derive from fuel oil storage tanks, T 80 C, T 80 D, MCC 20 KV, SHEQ and admin office, ware house, gas compressor area, gas engines, combustion turbine, control room, centrifuge pump, and regent heater. Active fire protection was several assessments such as alarm, detector, sprinkler, exhaust, and hydrant. On passive fire protection, the assessment was based on buildings. Field observations obtained the following results active protection system such as alarm with enough categories, detector with enough categories, sprinkler with enough categories, a fire extinguisher with a good category, hydrants with good categories and on passive fire protection systems with sufficient category.Keywords: fire protection, gas production, power plant
HUBUNGAN MOTIVASI, REWARD, DAN PUNISHMENT DENGAN PERILAKU TENAGA KERJA DALAM MENJALANKAN PROGRAM STOP DI PT X PASURUAN
Authors: Putri Risky Wira , Martiana Tri
Published: January 2019

Occupational Safety and Health (OSH) was an important benchmark in industrial activities in order to achieve maximum productivity and avoid losses that harm the company and the workers themselves. The number of work accidents in Indonesia awas still high. Mostly cause by human factors. One effort to prevent accidents that was STOP program with approach to human factor. Commitment of workforce in running STOP program has not reached 100% and STOP card collection still need to be reminded so that need to be improved by knowing factors related to worker behavior in running STOP program. One of them was by ABC analysis. This research type was observational with cross sectional research design with sample number 50 workers. Data collection by observation, give questionnaire, and interview to HSE. Methods of data analysis using spearmen correlation test. Independent variables in this study were reward and punishment. The dependent variable in this study was the behavior of the workers in running the STOP program. The result of statistical test shows that there was a relationship between reward (p = 0.000), and punishment (p = 0.039) with workers behavior in running STOP program. The conclusion of this research was gift reward and punishment can be increase behavior of the workers in running STOP program so reward and punishment can be used as consideration for program intervention.Keywords: ABC analysis, punishment, reward, STOP program
HUBUNGAN ANTROPOMETRI DENGAN KURSI KERJA DI KANTOR PELAYANAN PERBENDAHARAAN NEGARA MOJOKERTO
Authors: Andhini Vrilly
Published: January 2019

Chairs are an important component in determining comfort while sitting or doing a job with sitting. The aim of the research is to analyze the suitability between the size of the table and chair with anthropometry of the employee’s body. The number of respondents is 20 people. The variables of the study are measure of anthropometry and workstation. This is a descriptive research and using cross sectional approach. The measured variables are body anthropometry such as popliteal height, politeal but, hip width, shoulder height, shoulder width, elbow height. Variable work seats include seat height, seat base, length of seat base, width of seat, high backrest, width of backrest, elbow height. The results show that the dimensions of the workstation that corresponds to the body dimensions of the width of the seat base with the width of the hip the height of the backrest with the height of the sitting shoulder and the length of the elbow rest with the length of the forearm Dimensions that are inconsistent with the anthropometry of the body with the height of the chair with the popliteal height the length of the sitting base with popliteal buttock the width of the backrest with shoulder width the height of the elbow rest with the height of the sitting elbow. Conclusion is work chair is not accordance with anthropometry of employees because only 3 dimensions of work chairs are compatible with employee anthropometry.Keywords: anthropometry, chair, dimensions
FAKTOR YANG BERHUBUNGAN DENGAN DERMATITIS KONTAK AKIBAT KERJA PADA PEKERJA PROYEK BANDARA
Authors: Wardani Harumi Kusuma , Mashoedojo Mashoedojo , Bustamam Nurfitri
Published: January 2019

The incidence of contact dermatitis in Indonesia varies greatly and affects most workers. Epidemiological studied showed the incidence of Occupational Contact Dermatitis (OCD) was 0.5–1.9 cases/1000 workers/year. Aircarft Filling Depot Project at Juanda Airport is a construction project that is working on hydrant system and topping up development. This study was aimed at identifying risk factors of OCD among the project workers. A cross sectional design was used on 47 workers whom randomly selected during period May-June 2017. Data was collected using a questionnaire with the researcher’s guide. Occupational Contact Dermatitis is diagnosed by general practitioner that located in Naval Hospital (RUMKITAL) dr. Soekantyo Jahja Surabaya. Occupational contact dermatitis risk factors were analyzed using Chi-square test, followed by multiple logistic regression test. Of 47 workers, 21 (43.8%) experienced OCD. The use of personal protective equipment (PPE), duration of contact, personal hygiene, and history of skin diseases were associated with OCD (p < 0.01), whereas the type of work was not related to OCD (p = 0.491). The most dominant factor causing OCD was personal hygiene (OR = 9.659), followed by duration of contact (OR = 8.576), and history of skin disease (OR = 3.420). In conclusion, factors of use of PPE, length of contact, personal hygiene, and history of skin relationship with DKAK, while the type of work is not related to DKAKKeywords: aircraft filling depots project, occupational contact dermatitis, risk factor
EVALUASI PEMASANGAN DAN PEMELIHARAAN ALAT PEMADAM API RINGAN DI GRESIK
Authors: Wardana Rizki Prasetya
Published: January 2019

Oil and gas industry is an industry sector that has a high risk of fire. Fire is a potential hazard which can caused material loss, break of the production process and the danger to the human life safety. The aim of this research is to evaluate the installation and maintenance of portable fire extinguisher in PT. Pertamina Lubricants Production Unit Gresik. This research was observational and descriptive research using cross sectional design. The data used are primary data obtained from observations and interviews, and secondary data from documents and other supporting reports. The result showed that management has provided portable fire extinguisher almost every corner of the office building and production areas. The results of the identification of a potential fire hazard in PT. Pertamina Lubricants Production Unit Gresik that there is a potential fire hazard group A (solid materials, except metal), group B (material flammable liquid or gas) and group C (voltage electrical installations). Variables which have been in accordance with Decree of Minister of Labor No.04/MEN/1980 is the type and condition, within the installation, and the suitability of portable fire extinguisher signs, while variable that does not fit is the mounting height, sign mounting height and maintenance of portable fire extinguisher. The conclusion is PT. Pertamina Lubricants Production Gresik Unit has carried out the installation and maintenance of portable fire extinguishers but some are not in accordance with the regulations.Keywords: fire, installation, maintenance, portable fire extinguisher
RISK ASSESSMENT ALAT PRODUKSI GULA CANE KNIFE PADA STASIUN GILINGAN DI PT. X
Authors: Putra Bramantio Andananta Chrisna
Published: January 2019

Industrial development in Indonesia is growing rapidly. This should be balanced with the application of good HA safety risk management to workplace hazard risks. As in PT. X Lumajang Regency, in its production process has great potential hazard. One of them is in the cane knife tool at Gilingan Station that has been damaged in operation and has an employee accident impact. Therefore, to prevent the occurrence of recurrence of risk assessment, including hazard identification, risk assessment and hazard control. The purpose of this study is to conduct risk assessment using the method of failure mode effect analysis (FMEA) on the Cane Knife production process tool in PT. X Lumajang District. This research is descriptive research. The target of this research is sugar production process at PT. X with the informant is 1 person Quality Control (QC), 1 person production operators and 1 supervisor. The results show that there are 6 (six) failure modes that occur in the cane knife tool. Based on the Risk Priority Number (RPN), the highest failure mode occurs is a broken clutch string. While for risk control, quite enough. This is because there have been risk control efforts include technical control that is the installation of safety valve, administrative control is the application of SOP for workers and personal protective equipment. But for personal protective equipment only safety helmet, for other companies do not provide. The conclusion that can be drawn is the need for better risk control, especially on the damage that occurred on the clutch rope broken tool cane knife.Keywords: cane knife, failure mode effect analysis, risk assessment
ANALISIS TINGKAT KELELAHAN SUBYEKTIF BERDASARKAN SIKAP KERJA PENJAHIT DI INDUSTRI KONVEKSI
Authors: Izzati Triana , Ardyanto W., Denny
Published: January 2019

Work manner affects health safety and environment in the work station. It also affects workers’ fatigue which can reduce their performance resulting from the excessive of physical and mental at the working environment. The purpose of this study was to analyze the relationship between the tailor work attitude with the incidence of work fatigue on workers in the Ramli convection industry that engaged in fashion. The research was a descriptive by using the assessment of RULA model that targets of body posture of the workers in the Convection Industry. This research aimed to estimate the risk accident of skeletal muscle disorder and to assess work attitude which having pain, fatigue and musculoskeletal disorder by using RULA Model. The improvement of work attitude with changing of work position by standing up and sitting down, not slouching and not bending, and the improvement of work station such as making the ergonomic of the tailors’ chair. The results showed a strong correlation between fatigue with age (r = 0.711), work period (r = 0.854), intermediate relationship with gender (r = 0.439), work attitude (r = 0.416), and the working environment (r = 0.419). The result showed a weak correlation between fatigue with work station (r = 0.448). The conclusion is workers who have a high risk of work attitude experience a higher level of fatigue.Keywords: work attitude, work fatigue, work station
STUDI FAAL PARU DAN FAKTOR DETERMINANNYA PADA PEKERJA DI INDUSTRI SAWMILL
Authors: Hikmayanti Ulfa
Published: January 2019

Sawmill industry processes log into sawmill and also results wood dust as by-product . Dust can be potentially inhaled by workers and cause impaired lung function. In addition, several other factors can effect lung function such as age, body mass index status, smoking habit and using respiratory protective equipment habits. The purpose of this study was to describe lung function on workers in sawmill industry and the determinant factors. This research was an observational type with descriptive method. This study used total population with 36 workers. The data were obtained from interview, dust measurement, body mass index measurement and lung function test, meanwhile the instruments were questionnaire, high volume dust sampler (hvds), microtoise, weight scale and spirometer. This data were analyzed by cross tabulation. The results of this study showed that the time weighted average concentration in UD. Sinar Abadi was 1.59 mg/m3, meanwhile spirometry test showed 42.8% had normal lung function and 52.8% had impaired lung function. Workers who had impaired lung function were 64.7% exposed to doses >5mg/m3.tahun, 60% were >44 years old, 100% were obese, 69.9% were smoker and 76.5% never used respiratory protective equipment. The time weighted average concentration was above from threshold limit value and most of the workers had impaired lung function. Workers with impaired lung function are more likely to be exposed to dose >5mg/m3.years, older, obese, smoker, and didn’t use respiratory protective equipment. Keywords: determinant factors, lung function, wood dust
PEMANFAATAN LIMBAH KOTORAN SAPI DAN JERAMI KACANG TANAH SEBAGAI BOKASHI CAIR BAGI PERTUMBUHAN TANAMAN SAWI (Brassica juncea L.)
Authors: Artiana Artiana , Hartati Lilis , Sulaiman Abrani , Hadie Jamzuri
Published: January 2019

Has conducted a study entitled " Cow Manure Waste Utilization and Straw Peanut For Liquid Bokashi For Plant Growth mustard (Brassica juncea L.)". This study aimed to analyze the nutrient content in the liquid Bokashi is derived from cow dung and straw peanuts, and study the effect of dosing Bokashi different liquid to the growth of the mustard plant (Brassica juncea L.). Methods using a completely randomized design with one factor at a dose of 125 ml, 250 ml, 375 ml and 500 ml, and as control using Bokashi solid. This research was conducted with four replications. Bokashi liquid nutrient content of C of 0,1045%, 0,0461% of N, P and K amounted to 0.0172% at 0.2500%. The content of nutrient dense Bokashi C of 6,0874%, 2,0169% of N, P and K amounted to 0,0218% at 5,7802%. Bokashi dosing different liquid to the growth of mustard (Brassica juncea L.) significant effect on the growth of mustard, but still lower than the solid Bokashi. Dose liquid Bokashi most optimal for growing mustard (Brassica juncea L.) is at 375 ml.
ANALISIS KESESUAIAN WISATA DIVING DI KAWASAN PERAIRAN PULAU KUNYIT SEBELAH TIMUR KECAMATAN PULAU LAUT TANJUNG SELAYAR KABUPATEN KOTABARU
Authors: Koriyandi Abdul , Hamdani Hamdani , Salim Dafiuddin
Published: January 2019

The waters of Kunyit Island on the east is one of the coastal waters in Kotabaru Regency that has the potential for marine tourism. However, in development is still a lot of obstacles, including the suitability of land for marine tourism have not been identified. Utilization of coastal and marine resources in the waters of Kunyit Island on east may be the development of marine tourism such as diving. The purpose of this study was to determine the suitability of land suitability level of marine tourism in the waters of Kunyit Island on  east based on physical oceanographic parameters and BioEcology. The method used is the descriptive method with the help of analysis Travel Suitability Index (IKW) which generates the conformity travel. The results of this study indicate that the results of marine tourism suitability for diving in a class quite suitable (S2) with an area of 17.44 ha (78.72%) and is not suitable (N ) with an area of 4.71 ha (21.28%).
PERUBAHAN SOSIAL MASYARAKAT DI PERDESAAN HUTAN RAWA GAMBUT (Kajian Alih Guna Hutan Rawa Gambut Menjadi Perkebunan Kelapa Sawit di Kecamatan Marabahan Kabupaten Barito Kuala)
Authors: Nurhidayati Nurhidayati , Hafizianor Hafizianor , Muhammad Muhammad , Fauzi Hamdani
Published: January 2019

The purposes of this study are to 1) study the history, process, and factors of the background of the conversion of wetland forests into palm oil plantations, 2) analyze the social changes that occur in the aspect of population (demography), which includes changes in population structure and processes of the population, 3) to analyze the social changes that occur in the socio-economic aspects of forest villagers concerning domestic economic conditions of the society and the contribution of forest resources to the community, 4) analyze the social changes that occur in the socio-cultural aspects of rural community that include the status and social roles, social stratification and social mobility. This research used a quantitative approach with the questionnaires which were analyzed by Wilcoxon Match Pairs, Differential test and quantitative analysis techniques Enumeration. This study was carried out in two villages: Antar Raya and Antar Jaya which are located in Marabahan, Kabupaten Barito Kuala. The results showed that: 1) the history and the process of changing over the peat swamp forests into oil palm plantations began to exist in both places since 2008. The peat boglands were converted into oil palm plantations  which were formerly forests for public rice farming, and looking for Galam wood, rattan, and  fish. The background factors of the function changes were among others: a) the untapped potential of peat swamp forests, so that local governments and entrepreneurs were willing to use the land, b) people who wanted to have more developed and prosperous lives; c) public expectations that the opening of employment opportunities as well as obtaining compensation or redressing land ownerships; 2) changes in society due to the conversion of the peat swamp forests into oil palm plantations, which caused social changes  occur in the aspect of population (demographics); 3) social changes that occurred in the socio-economic aspects, among others: a) the state of the economy of the forest village communities; b) the role of forest resources for the community; 4) social changes that occurred in the socio-cultural aspects, namely a change occurs in status and social role, changes also occurred in the social stratification, and changes also occurred in the social mobility of families.
PEMANFAATAN DATA SATELIT TROPICAL RAINFALL MEASURING MISSION (TRMM) UNTUK PEMETAAN ZONA AGROKLIMAT OLDEMAN DI KALIMANTAN SELATAN
Authors: Noor Riza Arian , Ruslan Muhammad , Rusmayadi Gusti , Badaruddin Badaruddin
Published: January 2019

The irregularity of observation sites distribution and network density, lack data availability and discontinuity are the obstacles to analyzing and producing the information of agroclimate zone in South Kalimantan. TRMM satellite needs to be researched to overcome the limitations of surface observation data. This study intended to validate TRMM 3B43 satellite data with surface rainfall, to produce Oldeman agroclimate zone based on TRMM satellite data and to analyze the agroclimate zone for agricultural resources management. Data validation is done using the statistical method by analyzing the correlation value (r) and RMSE (Root Mean Square Error). The agroclimate zone is classified based on Oldeman climate classification type. The calculation results are mapped spatially using Arc GIS 10.2 software. The validation result of the TRMM satellite and surface rainfall data shows a high correlation value for the monthly average. The value of correlation coefficient is 0,97 and 25 mm for RMSE value. Oldeman agroclimate zone based on TRMM satellite data in south Kalimantan is divided into five climate zones, such as B1, B2, C1, C2, and D1.
ANALISIS KANDUNGAN PHOSPAT (PO4) DAN NITRAT (NO3) DI SUNGAI KARANG MUMUS SAMARINDA
Authors: Pramaningsih Vita , Suprayogi Slamet , Purnama Ig. L. Setyawan
Published: January 2019

Estuary of Karang Mumus River is Mahakam River that used for drinking water substance in around Samarinda Province East Kalimantan. There are communities live along floodplain area and use water in the river for daily activities as the bath, washing, and privy. Karang Mumus River through the urban area with complicated activities. Domestic waste content in Karang Mumus River come from the communities activities. It content nutrition as Phosphate and Nitrate reason booming algae in the tributary. It happens to impact the water pollution since less Dissolved Oxygen (DO). Purpose this research is analysis distribution of Phosphate and Nitrate in Karang Mumus River. The method used surveys as observation, water sampling, and laboratory analysis. Measurement spot is 17 from upstream to downstream. The result Nitrate is excellent but Phospat high on some spot measurement. Especially it happens in part of urban, market and several communities. The highest Nitrate is 2.13 mg/L standard 10 mg/L and highest Phosphate is 0.86 mg/L standard 0.2 mg/L.
PEMANFAATAN LIMBAH PELEPAH DAN DAUN KELAPA SAWIT MELALUI FERMENTASI Trichoderma sp. SEBAGAI PAKAN SAPI POTONG
Authors: Rizali Akhmad , Fachrianto Fachrianto , Ansari M. Hafiz , Wahdi Anis
Published: January 2019

The dependence on imports of feed ingredients for ration composer are increasingly expensive and availability of limited and unsustainable local feed, causing the low level of production and reproduction of local Indonesian cattle. This study aims to exploit the potential of plantation waste as an alternative feed of beef cattle, increase the nutrient value and digestibility of waste of palms and leaves and to know the optimal use of inoculums (Trichoderma sp.) through fermentation in improving the digestibility and nutritional value of the feed. The research method used was a complete randomized design (RAL) with five treatments and three replications, with 14 days fermentation. The research treatment includes: PD 0 (without Trichoderma/control), PD 1 (fermentation using Trichoderma viride 3 ml), PD 2 (fermentation using Trichoderma viride 6 ml), PD 3 (fermentation using Trichoderma harzianum 3 ml), PD 4 (fermentation using Trichoderma harzianum 6 ml).O Observation parameters observed included dry matter (DM), crude protein (CP), ash content (AC), coarse fat (CF), organic matter (OM), and total digestible nutrient (TDN). The data obtained were analyzed using variance analysis. The results showed that the best treatment was found in PD 1, had a significant effect on control in increasing total digestible nutrient (TDN) 51.62% and crude fiber decline by 39.14%, although an increase in CP (5.34%) was not equal to the treatment of the PD 4 (5.35%). While the best increase of CP content was found in the treatment of PD 4 of 3.35%. While the best CP content found in the treatment of PD 4 of 5.35%. It was concluded that the use of Trichoderma viride and Trichoderma harzianum can improve the quality of waste nutrient and palm oil leaves by fermentation and the optimal inoculums dose used to produce the best fermentation is the use of Trichoderma sp. 3 ml in 3kg of material.