bluuebunny/crossref_metadata_2025 huggingface dataset:
INSTITUTIONAL MODELLING OF THE MANAGEMENT SYSTEM FOR REGIONAL PARTICIPANTS OF DUAL EDUCATION
Authors: Kokovikhin A. Yu. , Mokronosov A. G. , Ogorodnikova E. S.
Published: March 2019

Introduction. The need for increasing the investment attractiveness of Russian regions and meeting the needs of territories for qualified personnel updates the search for infrastructure and coordination mechanisms and tools of the solution of these problems. At the same time, the diversity of interests of participants in dual education and their various structural positions do not allow to solve this task by directive methods, both on the part of state structures and corporate sectors. One of the solutions might be to form an effective management system for dual education, which assumes the organisation of practical vocational training according to inquiries of employers, who are involved in implementation of educational process in order to promote labour market with in-demand experts. The aimof the present research was the scientific substantiation of the choice of the institutional model for management of dual education system at the regional level, taking into account the interests of all its members: enterprises, government agencies, students and educational institutions.Methodology and research methods. The research was based on the systematic approach. The methods of modelling and comparative analysis were employed. The provisions and principles of utility theory served as the methodological basis of institutional model formation.Results and scientific novelty. The main target reference points of regional participants of dual education were identified and formalised in the form of certain indicators. Two types of models of management of these subjects were considered: models with and without the usage of institutional superstructures. As a key criterion for model selection, maximization of a complex indicator of its utility for interested parties was offered. In particular, it was demonstrated that supervision of graduates by a certain employer is caused by parameters of employment attractiveness, such as a wide range of horizontal and vertical career cards and a possibility of continuing vocational education. The conducted quantitative research on utility indicators allowed the authors to design the institutional model for management of regional participants within the system of dual education and to recommend it for further implementation.Practical significance.The institutional model presented in the publication is directed to the solution of tasks faced by partner companies and educational establishments within the system of dual education. When applying this model, it is possible to forecast personnel requirements – job availability in the future of jobs, resulting to a considerable simplification of vocational guidance  work, which can be carried out in the course of applicants’ preparation.
El sosiego un valor esencial ´para el cuidado de enfermería
Authors: Lagoueyte Gómez María Isabel
Published: March 2019

Este artículo tiene como objetivo reflexionar sobre la necesidad de tener una actitud de sosiego, silencio y respeto que contribuya al bienestar y a la recuperación de los pacientes hospitalizados
Atención de enfermería frente al manejo postoperatorio de fracturas oseas.
Authors: Lugo Ruiz Alfredo José , Meléndez Mogollón Isabel Cristina , Andrade Mario Andrade , Córdova Yomar
Published: March 2019

Objetivo: Describir la atención de enfermería en pacientes pos operados por fracturas óseas en humero, tibia y peroné, a fin de optimizar el cuidado del paciente durante la hospitalización dentro del Hospital de Especialidades San Bartolo.

Metodología: La investigación se enmarca en un enfoque cuantitativo, con un nivel descriptivo, de tipo no experimental, como un estudio bibliográfico, de campo. La técnica de recolección de datos empleada fue la encuesta, a través de un cuestionario de 15 preguntas cerradas, que fue aplicado a una población de 24 enfermeros (as).

Resultados: Entre los resultados más resaltantes, se evidencia desfavorablemente que el 100% de los encuestados omite cuidados, como lo es el control de líquidos a los pacientes, aunados a un 75% que no identifica los signos de alarma, no obstante, el 75% de la población considero que la administración de medicamentos es el cuidado principal en el postoperatorio mediato.

Conclusiones: Las fracturas óseas representan un problema de discapacidad funcional en los pacientes y es necesario que el cuidado de enfermería se dirija a satisfacer los requerimientos del paciente sin afectar la inmovilidad intencional que se establece como medida terapéutica operatoria
Modelado y simulación de planificadores de recursos radio para una red LTE
Authors: Quintero Florez Victor Manuel , Hernandez Bonilla Claudia Milena , Giraldo Medina Dario , Uribe Ante Diego Fernando
Published: March 2019

Una de las principales características de LTE es la capacidad para realizar la asignación dinámica de recursos de radio disponibles en el sistema, haciendo uso de diferentes algoritmos de planificación. Este artículo describe el modelado de los tres algoritmos de planificación de recursos de radio básicos en enlace descendente LTE conocidos como Round Robin, Proportional Fair y Maximun Rate, su implementación en el Simulador Básico a Nivel de Sistema, y la simulación de un escenario específico para evaluar el rendimiento de cada algoritmo en capacidad (throughput) para una red LTE. Para cumplir con tal propósito se emplea una metodología lineal secuencial, empezando con una breve descripción de la tecnología LTE para contextualizar al lector, seguidamente se detallan las fases de diseño e implementación y finalmente se realizan procesos de simulación y pruebas en dos escenarios LTE específicos, con el fin de evaluar el desempeño de cada uno de los planificadores de recursos radio implementados y determinar el impacto que tienen estás técnicas en la capacidad (throughput) que se puede llegar a alcanzar en una red LTE.
Editorial - Estímulos a la productividad académica en la Universidad Libre
Authors: Rios Alvarado Arnaldo
Published: March 2019

Editorial
Retornos de la educación para los trabajadores formales e informales en Cali
Authors: Ordóñez Castaño Iván Andrés , Sanabria Domínguez Johana Andrea
Published: March 2019

En este artículo se analizan los retornos de la educación para empleados formales e informales con diferentes niveles de ingreso en el municipio de Cali. Se utilizan los datos de la Encuesta de Empleo y Calidad de Vida (EECV) de 2013 para estimar una ecuación de salario con splines lineales, usando la técnica semiparamétrica de regresión cuantílica con corrección del sesgo de selección. Los resultados sugieren que la tasa de retorno de la educación secundaria y técnica en el sector informal supera a la del sector formal para todos los niveles de ingreso. En contraste, con un nivel de educación superior es más rentable emplearse en el sector formal. En conclusión, se corrobora que el rendimiento marginal de la inversión en capital humano no es constante entre niveles educativos y tampoco entre niveles de ingresos.
Las instituciones de guarda y cuidado en el ordenamiento familiar cubano. Pautas y retos
Authors: Galiano-Maritan Grisel , Noa-Pérez Hanny , Morffi-Collado Claudia Lorena
Published: March 2019

El presente trabajo versa sobre la necesidad de regular en el Código de Familia cubano aquellas instituciones jurídicas de guarda y cuidado para complementar la capacidad de aquellas personas carentes de ella, o que la poseen, pero con limitaciones, en aras de impedir su indefensión. El objetivo es fundamentar los presupuestos teóricos normativos sobre las instituciones de guarda y cuidado en el ordenamiento jurídico familiar cubano. Para ello se emplearon métodos científicos, y técnicas propias de las ciencias jurídicas, dentro de ellos el histórico – lógico, exegético – analítico, jurídico comparado; y la técnica de investigación empleada fue el análisis de documentos. Se trata de un estudio eminentemente teórico, el tipo de investigación es descriptiva y la metodología empleada, cualitativa. Este estudio arrojó como principal resultado la necesidad de regulación de las instituciones de guarda y cuidado en el Código de Familia cubano ante la existencia de sujetos del Derecho carentes de capacidad de obrar, o con capacidad restringida
Sobre la conformación de una comunidad discursiva
Authors: Savio Karina
Published: March 2019

En 1948, Arminda Aberastury constituye el primer grupo de psicoanalistas de niños en la Asociación Psicoanalítica Argentina, única institución oficial en ese entonces. Este artículo se propone estudiar la incipiente conformación discursiva de este grupo a la luz del concepto de comunidad discursiva, tomando como referencia los artículos que se publican sobre esta temática en la Revista de Psicoanálisis, principal instancia difusora de la institución. Para ello, se analizan tres ejes: la configuración de las introducciones, el empleo del ‘nosotros, los psicoanalistas’ y las modalidades que adquieren la transmisión del saber teórico y clínico. El recorrido emprendido revela ciertas continuidades y rupturas discursivas que dan cuenta de una identidad grupal incipiente y en formación.
Buen trato a la infancia
Authors: Contreras-Ramírez Roberto , Vega-Arce Maribel
Published: March 2019

La deriva semántica de los conceptos en la producción científica muchas veces significa una puerta de entrada para las diversas representaciones colectivas y su praxis consecuente. Anterior a la difusión de las palabras mismas y su asimilación en el imaginario colectivo en la vida cotidiana, cada una de ellas muestra un curso de validación dentro de la comunidad científica. El buen trato a la infancia es un concepto emergente que comienza a ser nombrado en 1997, frecuentemente asociado a prácticas de cuidado en salud o protección social, creado por oposición y en prevención al maltrato infantil. Mayormente presente en el mundo francés e hispano, esta noción logra un importante impacto en la comunidad científica latinoamericana. El propósito de este estudio es mostrar el comportamiento que presenta el despliegue del concepto en las principales revistas indexadas en la red de Redalyc durante los últimos quince años. Desde un análisis de orientación bibliométrica del término se compara la participación de países latinoamericanos de habla hispana en la publicación de artículos que usan el término en referencia al mismo como fenómeno presente en diversos campos y disciplinas. A partir del reconocimiento de esta distribución en el tiempo, se develan los principales significados y referencias del concepto. Se espera, a partir de este mapeo, contribuir a la dilucidación y delimitación del mismo, para aportar a su validación dentro y fuera de la comunidad científica.
Protección social de las personas adultas mayores: Inclusión y equidad en Argentina durante el periodo 2003-2014
Authors: Trujillo-Salazar Lucía
Published: March 2019

En este artículo se reflexiona sobre las transformaciones del sistema previsional argentino durante el periodo 2003-2014, para abarcar las políticas de protección de los ingresos implementadas durante los gobiernos Kirchneristas. El objetivo principal es brindar evidencia empírica acerca de cómo las políticas implementadas impactaron en una mejora sustantiva de los niveles de cobertura y distribución del ingreso. El trabajo se desarrolla a partir de los datos de la Encuesta Permanente de Hogares. Entre los principales hallazgos es importante destacar que la estructura social se vio modificada al registrarse una expansión de la cobertura que, además en términos relativos, se concentró en la parte baja de la distribución del ingreso, es decir en los hogares más vulnerables. Asimismo, el mayor incremento relativo en la percepción de ingresos jubilatorios, en la parte baja de la distribución, se tradujo en mejoras distributivas del ingreso. Sin embargo, las políticas que se implementaron y transformaron el funcionamiento de la protección social en el sistema jubilatorio, requieren ser repensadas en una estrategia sustentable de largo plazo.
sostenibilidad del capital territorial: propuesta metodológica para su análisis y valoración
Authors: Trujillo Osorio Catalina , Eraso Torres Francisco Wilfredo , Loaiza Trejos Paola Andrea
Published: March 2019

Se presenta una propuesta metodológica para evaluar la sostenibilidad de los procesos de desarrollo regional, e identificar sus efectos sobre los recursos naturales, considerando el desarrollo sostenible como el mejoramiento de las condiciones humanas mediante el mantenimiento del sistema socioecológico. Se desarrolla el concepto “capital territorial” como marco referencial para evaluar la sostenibilidad temporal y transversal del desarrollo; incorporando el principio de no sustituibilidad entre capitales; e identificando las condiciones de sostenibilidad que deberían satisfacer los procesos de desarrollo.  El artículo aborda tres aspectos. Primero, a partir de la conceptualización del desarrollo sostenible se propone el capital territorial como metodología de análisis, segundo, se construye una metodología de valoración cuantitativa para la sostenibilidad parcial y total de los capitales; y tercero se evalúa la sostenibilidad del capital natural de Colombia, utilizando la variable porcentaje de área departamental con cobertura natural vegetal.  Se encontró que el uso del suelo actual y la cobertura natural vegetal del país presentan tendencia negativa (-0.06), evidenciando la insostenibilidad de los procesos y políticas de desarrollo sobre los bosques del país. Estos resultados señalan la urgente necesidad de armonizar el crecimiento económico con la conservación de bosques y ecosistemas naturales. Esta metodología puede ser aplicada a cualquier capital y proceso de desarrollo, constituyendo una herramienta de valoración práctica para evaluar la sostenibilidad de los recursos naturales y sociales de un territorio.
Capacidad solubilizadora de fosfato de aluminio por hongos rizosféricos aislados de un Andisol colombiano
Authors: Callejas Cañarte Gina Vanessa , Cisneros Rojas Carlos Adolfo , Caicedo Bejarano Luz Dary
Published: March 2019

A partir de un Typic Melanudands cultivado con café (Coffea arabica variedad Caturra), proveniente del Departamento del Cauca (Colombia), se aislaron hongos con capacidad solubilizadora de fosfato de aluminio (Al-P), de los cuales se seleccionaron dos microorganismos que presentaron la mayor actividad, identificados como cepa UNH1 (Mycelia sterilia), y UNH2 (Penicillium sp).  Los hongos seleccionados fueron evaluados por quince días en medio líquido Pikovskaya (PVK) con Al-P, bajo dos condiciones, con agitación (método A) y en reposo con aireación (método B). Al comparar los resultados se encontraron diferencias estadísticas significativas en el porcentaje de fósforo soluble, el método A presentó mejores resultados con un porcentaje de 73,8% frente a 62% con la cepa UNH1 y en menor proporción con la cepa UNH2 49% y 44%. El incremento de biomasa fue mayor con el método B y el pH de los medios de cultivo no mostraron diferencias significativas, con un pH promedio de 2,7.
proceso de inteligibilidad espacial. Una propuesta tipológica de los espacios subjetivos en las ciudades
Authors: Pyszczek Oscar Luis
Published: March 2019

El abordaje de las urbes del siglo XXI supone un cambio en las perspectivas analíticas de estudio, mediadas por el enfoque ecológico, que permite considerar a las ciudades como ecosistemas eminentemente antrópicos con mecanismos de defensas colectivos, entre ellos, la inteligibilidad espacial. El presente artículo procura poner en discusión la atribución de significados a diferentes espacios intraurbanos principalmente de ciudades latinoamericanas. El objetivo principal consiste en la identificación de la cartografía invisible subyacente, esgrimiendo una aproximación tipológica de espacios subjetivos con base en la pervivencia de sensaciones, percepciones y estigmas urbanos. La metodología supone una doble vía de aproximación: una teórica, resultado de la búsqueda, selección, análisis y sistematización de los trabajos referidos a las cartografías urbanas invisibilizadas, principalmente de América Latina. Se procura identificar las correspondencias entre las experiencias e investigaciones, cuyo resultado es el diseño de una clasificación de espacios subjetivos. En una segunda instancia y de modo empírico, se exponen casos que sustentan y ejemplifican la categorización de espacios subjetivos surgidos en la etapa previa El fin del trabajo científico, consiste en debatir sobre el acervo inmaterial que converge y circula dentro y fuera de los espacios urbanos.
“Clan del golfo”, una amenaza para el acuerdo de paz con las FARC-EP. Un acercamiento desde el fenómeno de la convergencia
Authors: García-Perilla Juan Carlos , Rodríguez-Beltrán Carlos Andrés
Published: March 2019

La implementación del proceso de paz con las FARC-EP por parte del gobierno tiene múltiples amenazas, entre ellas la existencia de un grupo armado ilegal denominado el “Clan del Golfo”, el cual se originó después de la desmovilización de las Autodefensas Unidas de Colombia. Desde el fenómeno de la convergencia se caracterizará y analizará al “Clan” comprendiendo cómo su accionar político, social, económico y militar afecta de forma negativa la ejecución de algunos de los puntos suscritos en el acuerdo de paz, transformándose en una amenaza a los acuerdos y a la seguridad nacional del Estado colombiano.
Germinación y crecimiento inicial de Hymenaea oblongifolia Huber en el municipio de Istmina, Chocó, Colombia
Authors: Torres-Torres Jhon Jerley , Medina-Arroyo Henry Hernan , Martínez-Guardia Melida
Published: March 2019

La tala selectiva de los bosques ha ocasionado que las densidades de Hymenaea oblongifolia Huber se hallan disminuido significativamente en el departamento del Chocó. Por lo que se hace necesario avanzar en estudios que aporten a su manejo. El objetivo de esta investigación fue evaluar la germinación, el crecimiento inicial y la supervivencia de esta especie empleando semillas silvestres. En cada uno de los ambientes: libre exposición solar LS y bajo sombra BS, se utilizó un diseño completamente aleatorizado, con arreglo factorial, conformado por 12 tratamientos y tres repeticiones de cuatro semillas cada una. Se emplearon dos combinaciones de sustratos orgánicos en presencia de un testigo (Arena aluvial). Para las variables germinación y altura no se detectaron diferencias estadísticas significativas; mientras que para diámetro si se presentaron diferencias. Los valores de germinación fueron mayores en LS (67,4%) que BS (65,3%), presentándose la misma tendencia para la supervivencia (SL= 82% y BS=81,2). Por lo contrario, para diámetro y altura el mejor comportamiento de las plántulas se presentó BS con valores de 0,38 cm y 17,94 cm respectivamente.  Se recomienda germinar las semillas de H. oblongifolia a libre exposición solar y pasar las plántulas a sombra para su crecimiento inicial en vivero.
Rebuscadores de la calle: A Photograph of the Working Poor in Bogotá
Authors: Porras Laura
Published: March 2019

Using qualitative methodologies, this paper offers a contribution to the very recent literature on rebusque by characterizing the practice of the social grouping that I will refer to as «street rebuscadores» in Bogotá. Based on eighteen months of ethnographic research in the localities of Ciudad Bolívar and Suba (2012-2014), this paper draws on Bourdieu’s theory of practice, particularly on the concepts of habitus and capital, to argue that street rebuscadores share a similar volume and composition of overall capital (or habitus), as well as various practices associated with their habitus. Within that theoretical framework, this paper describes and analyses four common practices of street rebuscadores, in the hope of shedding some light on the logic underneath those practices. Ultimately, my political goal is also to give some visibility to the most vulnerable segment of the working poor.
Revisión narrativa de la relación entre envidia y Schadenfreude
Authors: Restrepo-Neira Cecilia
Published: March 2019

Envy and Schadenfreude are negative emotions. Envy promotes social comparison as well as the desire to have something that is possessed by another, while Schadenfreude involves a rewarding feeling derived from the pleasure obtained for misfortune happening to the envied person. Both emotions hinder social relationships. This narrative review is based on the state of the art on the relationship between envy and Schadenfreude. A review of these concepts was carried out and social psychology and neuroscience experimental studies were analyzed. The findings indicate that the literature reviewed focuses on four factors that relate envy and Schadenfreude: 1) brain activation, 2) social conditions, 3) empathy, and 4) the merit system. Additionally, the literature showed the possibility that envy could be a predictor of Schadenfreude, which would provide basis for new research studies to test this hypothesis.
Hearing Knowledge into Action: Mobilizing Sound for Multicultural Imaginaries
Authors: Doerr-Stevens Candance , Buckley-Marudas Molly
Published: March 2019

Drawing on multimodal, sound-based data, this study examines how high school students harnessed elements of sound and music for multicultural learning within collaborative research and radio podcasting. Data were collected from a variety of sources, including field notes, final media projects, and audio and video footage of students’ collaborative media production processes and interviews. Findings reveal multivocal and divergent engagements in the sound editing process as well as multimodal struggles in which students leveraged sound to express nuanced views about racism, culture, and privilege. This study has implications for educators teaching multicultural perspectives and critical media literacy studies.
Intranasal Neurostimulation for Dry Eye: First Impressions
Authors: Faulkner William J , Granat Stephanie
Published: March 2019

Although many new and helpful treatments for dry eye disease have been developed, there remains a subset of patients who continue to look for easier or more effective treatments. A consecutive group of 28 moderate to severe dissatisfied dry eye patients in one practice were surveyed after initial exposure to and trial with a novel intranasal neurostimulation technique. Results were overall positive. Seventy-one percent had a favorable impression. Average rating on 1-10 scale was 6.92. Twenty-five percent purchased the device immediately despite no insurance coverage. Of those who did not buy, 100% said they would consider purchase later. Time and experience will show how valuable this new technology is and how it fits in the dry eye armamentarium.
Developing nationalism character-based children's literature module to increase Islamic elementary school students' critical thinking
Authors: Al Fikri Hajarudin
Published: March 2019

This study aims at producing a literature module of children based on nationalism character to improve the critical thinking ability of fourth grade students MIN Boyolali, and measuring the effectiveness of the module use in classroom toward critical thinking ability of fourth grade students MIN Boyolali. Set within the research and development type, this study adapts 10 (ten) stages of development procedure as formulated by Borg and Gall (1983). The subjects of the research were students and teachers of grade IV MIN Boyolali. Data collection was conducted through interview and observation, product validation, teacher and student response questionnaire, and performance of pre-test and post-test—which was analyzed using independent sample t-test. Meanwhile, product effectiveness was tested through quasi experiment. The result of the research showed that the module was categorized as “fit” by material and language expert; while the media expert categorized it as "very fit". In the other hand, teacher and student responses to the use of learning module were categorized as "excellent". Further, the output of the independent sample t test showed that the p value (0.000) was less than the 0.05 significance level (p≤0.05), indicating that the module was effective to improve students' critical thinking skills.
Cultural literacy building of primary school students as basic concept
Authors: Sobirin Ma'as , Susapti Peni
Published: March 2019

The low literacy ability of the society will have an impact on the quality of human resources. Nowadays, literacy (read-write) has not yet become a necessity of life and part of nation's culture. Schools as the primary means of shaping the character of students need to plan programs that lead to literacy culture as a solution to the problem. Currently, schools are busy implementing government programs through literacy programs with the aim of the growing awareness of students in familiarizing the culture. This program provides the significant influence for students with various forms of activities undertaken by school. On this occasion, the writer tries to present some explanations related to the basic concepts, urgency, stapes of cultural development of literacy and the form of programs that can be done in supporting of literacy culture in Elementary School.
Inhibitors of N-glycosylation as a potential tool for analysis of the mechanism of action and cellular localisation of glycoprotein P.
Authors: Wojtowicz Karolina , Szaflarski Witold , Januchowski Radosław , Zawierucha Piotr , Nowicki Michał , Zabel Maciej
Published: March 2019

Multidrug resistance has for many years attracted attention of numerous investigators. Attempts have also been made to increase efficiency of anti-neoplastic therapy. For this reason, most of efforts have been devoted to analysing proteins engaged in the mechanism of multidrug resistance such as the N-glycosylated membrane protein glycoprotein P. Interestingly, glycosylation probably plays a significant role in the intracellular location and activity of modified proteins. Inhibitors of glycosylation have been demonstrated to alter the activity of glycoprotein P in various ways, depending on the cell line examined. These inhibitors markedly reduce multidrug resistance of cancer cells, thus promoting success of anti-neoplastic therapy. Here, we review the basic knowledge on N-glycosylation inhibitors, their effect on glycoprotein P and their therapeutic potential.
Metagenomic approach in the investigation of new bioactive compounds in the marine environment.
Authors: Felczykowska Agnieszka , Bloch Sylwia Kamila , Nejman-Faleńczyk Bożena , Barańska Sylwia
Published: March 2019

The marine environment is estimated to be one of the most significant sources of biological activity in the world. In the last few decades an increase in the research intensity conducted on marine microorganisms has been observed, which confirms the great potential of these organisms in the field of bioactive compounds' production. In order to efficiently use the natural resources of the marine environment, metagenomics can be applied. This powerful technique allows for efficient screening of microbial biodiversity for bioactive compounds. The primary aim of this review is to present some aspects of the construction of metagenomic libraries, and strategies of screening for novel bioactives in the marine surrounding. This paper also illustrates several examples of the application of metagenomic methods in the discovery of novel enzymes and drugs in various marine environments.
Chemical composition of human and canine fascia lata.
Authors: Maksymowicz Krzysztof , Marycz Krzysztof , Szotek Sylwia , Kaliński Krzysztof , Serwa Ewa , Łukomski Robert , Czogała Joanna
Published: March 2019

The fascial system is an integral part of the musculoskeletal system. It is a three-dimensional network of connective tissue spreading ubiquitously throughout the body, surrounding muscles, bones, internal organs, nerves, vessels, and other structures. The basic biophysical properties of the fascial system are determined by its structure and chemical composition. This study aimed to determine the elemental composition of pathologically unchanged fascia lata of the thigh, collected during autopsies on humans and dogs. The wide spectrum of elements analysed included both macro and micro elements. The analyses were conducted using scanning electron microscopy with X-ray microanalysis (SEM-EDS). Concentrations of the following macro and micro elements were determined: C, N, O, Na, Mg, Al, Si, P, S, Cl, K, Ca, Ti, Fe Co, Ni, Cu, and Zn. The obtained results showed significant differences between human and canine fascia lata regarding the content of most of the examined elements (p < 0.05), except for N. These data may in future provide a starting point for the establishment of reference values for the content of various elements in normal fascial tissue and may also serve to verify the usefulness of experimental animal material as a substitute for human tissue.
Dexamethasone inhibits U937 cell adhesion via the down-regulation of ROCK1 activity.
Authors: Liu Dong , Chen Xing-Yun , Xiong Ren-Ping , Ning Ya-Lei , Li Ping , Peng Yan , Liu Ping , Zhao Yan , Yang Nan , Zhou Yuan-Guo
Published: March 2019

To explore the effect of dexamethasone (DEX) on monocyte adhesion function and its underlying mechanism.
The effects of DEX and fasudil on adhesion of cultured U937 monocytes to human umbilical vein endothelial cells (HUVEC) following stimulation with phorbol myristate acetate (PMA) were studied; Changes in the Rho-associated coiled-coil protein kinase 1 (ROCK1) protein content and activity were evaluated.
DEX and fasudil significantly inhibited U937 cell adhesion rates under PMA stimulation and inhibited ROCK1 activity. Mifepristone (RU-486) and cycloheximide (CHX) did not alter these effects of DEX.
DEX interferes with the adhesion function of U937 cells through the inhibition of ROCK1 activity.
Cytotoxicity of PP(Arg)2- and PP(Ala)2(Arg)2-based photodynamic therapy and early stage of apoptosis induction in human breast cancers in vitro.
Authors: Nowak-Stępniowska Agata , Wiktorska Katarzyna , Małecki Maciej , Milczarek Małgorzata , Lubelska Katarzyna , Padzik-Graczyk Alfreda
Published: March 2019

Mitochondria are cell energetic centers where ATP is produced. They also play a very important role in the PDT as intracellular sites of photosensitizer localization. Photosensitizers gathering in mitochondria (like porphyrin derivatives used in this work) are more effective in tumor cell destruction. Moreover, it was assumed that di-amino acid substituents attached to porphyrin ring will strengthen the effectivity of interaction with membrane receptors of examined cells. MTT assay was performed to investigate the influence of PP(Arg)(2) and PP(Ala)(2)(Arg)(2)-based PDT on breast cancer cell viability for 24 h, 48 h and 120 h after cell irradiation. Then the influence of PP(Ala)(2)(Arg)(2)- and PP(Arg)(2)-mediated PDT on early mitochondrial apoptosis induction via measurements of the transmembrane mitochondrial potential changes was examined. Results showed that lower energy doses and maximal nontoxic photosensitizer doses of PP(Ala)(2)(Arg)(2) and PP(Arg)(2) applied in PDT can imply apoptotic cell death. It was confirmed that modification of the protoporphyrin IX by attaching two alanine substituents raised the efficiency of photodynamic therapy.
The relationship between morphology and disaccharidase activity in ischemia- reperfusion injured intestine.
Authors: Varga Ján , Tóth Štefan , Tóth Štefan , Tomečková Vladimíra , Gregová Kristína , Veselá Jarmila
Published: March 2019

Questions regarding functional markers characterizing injured intestines remain unanswered. Brush border disaccharidases are crucial for the functioning of the intestines.
The study was designed to assess changes in disaccharidase activity (DA) following intestinal injury and to compare them with morphological changes.
Wistar rats, randomly divided into six experimental groups (each n = 6), were subjected to different ischemic/reperfusion injury. One-hour mesenteric ischemia followed by reperfusion for 0, 1, 2, 4, 12 or 24 hours was induced. As a control group sham-operated animals were used (n = 6). Intestine morphology was evaluated using histopathological injury index (HII) and goblet cell (GC) detection. DA (sucrase and maltase) was studied in mucosal scrape or in entire intestinal wall samples.
Moderate morphological damage (HII, GC) after mesenteric ischemia was detected. Deepening of the injury was found during reperfusion with a maximum after two hours. Improved morphology with longer reperfusion confirmed reversible damage with almost normal mucosal structure after 24 hours of reperfusion. Similar pattern was observed when DA was measured. The lowest activity was detected after 2 hours of reperfusion followed by increasing activity in the subsequent reperfusion periods. Physiological values after 24 hours of reperfusion were seen only in samples of entire intestinal wall.
Significant changes in intestinal DA were observed after intestinal ischemia/reperfusion injury. A similar pattern was seen for morphological characteristics. Although based on microscopic survey the intestine seems to be fairly regenerated, some functional limitation is expected to persist.
Dual, enzymatic and non-enzymatic, function of ecto-5'-nucleotidase (eN, CD73) in migration and invasion of A375 melanoma cells.
Authors: Sadej Rafal , Skladanowski Andrzej C
Published: March 2019

Ecto-5'-nucleotidase (eN, CD73) mediates extracellular adenosine production from 5'-AMP. Non-enzymatic functions of eN have also been reported. The aim of the study was to investigate the role of ecto-5'-nucleotidase in aggressive melanoma behaviour. Analysis of the involvement of eN in adhesion, migration and invasion revealed eN functions unknown to date. We found that following eN blockade by concanavalin A, the strength of adhesion to different ECM proteins was not altered, but at the same time the invasion ability of the cells was decreased. On the other hand, knocking down eN in melanoma cells did not influence cell invasion but abolished their migration on tenascin C (TnC). Ecto-5'-nucleotidase seems to fulfil a more distinct role as a receptor than as an enzyme in the cell interaction and mobility on TnC. Ecto-5'-nucleotidase activates also focal adhesion kinase and enhances the formation of complexes upon cell adhesion to TnC. All these observations prove that an eN-TnC complex is involved in cell migration and invasion and thus in the regulation of melanoma progression.
Assessment of the free binding energy of 1,25-dihydroxyvitamin D3 and its analogs with the human VDR receptor model.
Authors: Kamel Karol , Kolinski Andrzej
Published: March 2019

1,25-dihydroxyvitamin D(3) has quite significant anticancer properties, but its strong calcemic effect in principle excludes it as a potential anticancer drug. Currently, a lot of effort is being devoted to develop potent anticancer analogs of 1,25-dihydroxyvitamin D(3) that would not induce hypercalcemia during therapy. In this work, the free binding energy of the VDR receptor with 1,25-dihydroxyvitamin D(3) and its three potent analogs (EB 1089, KH 1060 and RO 25-9022) is calculated and compared with each other. With this approach, we could estimate the relative binding affinity of the most potent analog, RO 25-9022, and also revealed a quite distinct mechanism of its interaction with VDR.
Predictive factors for preeclampsia in pregnant women: a unvariate and multivariate logistic regression analysis.
Authors: Direkvand-Moghadam Ashraf , Khosravi Afra , Sayehmiri Kourosh
Published: March 2019

Several risk factors have been used to predict preeclampsia. The role of some risk factors as predictors associated with preeclampsia among Iranian women was analyzed in the present study using logistic regression.
610 women attending the obstetric ward of Mustafa hospital in Ilam were enrolled in this study. Demographic variables such as age, Body Mass Index (BMI), medical and obstetrics variables such as education, number of pregnancy, abortion and parity from May to September 2010 were analyzed. We used the unvaried and multiple logistic regression analyses to predict preeclampsia.
The history of preeclampsia, hypertension, and infertility showed to be good independent predicator variables for preeclampsia using multivariate logistic regression analysis (OR was 5.46, 2.34 and 3.07 respectively). Area Under the Receiver Operation Character (AUROC) was estimated to be 0.67 (95% CI 0.59-0.67, p
Sustained virologic response and IL28B single-nucleotide polymorphisms in patients with chronic hepatitis C treated with pegylated interferon alfa and ribavirin.
Authors: Jabłonowska Elżbieta , Piekarska Anna , Koślińska-Berkan Ewa , Omulecka Aleksandra , Szymańska Bożena , Wójcik Kamila
Published: March 2019

Hepatitis C virus (HCV) infection is a global health problem which can lead to liver cirrhosis or hepatocellular carcinoma in one-fifth of chronically infected patients.
The study group consisted of 123 patients: 90 with HCV mono- and 33 with HIV/HCV co-infection, who were treated with pegylated interferon alfa (Peg-IFN-α) and ribavirin. We analyzed selected pretreatment factors: age, sex, HIV/HCV co-infection, grade of inflammation, necrotic changes and fibrosis in histological analysis of liver bioptates, HCV viral load, HCV genotypes, and single nucleotide polymorphisms (SNPs) of IL28B and tried to find out which of them influence sustained virological response (SVR). The IL28B SNP C/T (rs12979860) was analyzed using Custom(®) SNP Genotyping Assays (Applied Biosystems).
Multivariate analysis demonstrated that after adjusting for the other variables three predictors independently influence SVR, namely genotype 3 of HCV, presence of the CC genotype and age >40 years (OR respectively 15.14, 3.62, and 0.36). HCV mono-infected patients were infected with HCV genotype 3 or 4 less frequently (p=0.0001) compared to HIV/HCV co-infected individuals. In patients with HIV/HCV co-infection the CC variant occurred more frequently whereas CT was found less frequently (p=0.001, p=0.0146, respectively). In patients with HIV/HCV co-infection, 3 and 4 genotype of HCV occurred more frequently compared to patients with HCV mono-infection (p=0.0001).
These data suggest that age, HCV genotype and IL28B polymorphism are useful for prediction of the response to treatment with Peg-IFN-α and ribavirin. The more frequent occurrence of HCV genotypes 3 or 4 in patients with HIV/HCV co-infection could be associated with the route of transmission.
Screening of environmental samples for bacteria producing 1,3-propanediol from glycerol.
Authors: Dąbrowski Sławomir , Zabłotna Ewa , Pietrewicz-Kubicz Dorota , Długołęcka Anna
Published: March 2019

Twenty nine environmental samples were screened for the presence of anaerobic microorganisms fermenting glycerol with 1,3-propanediol as a final product. Seven samples were then selected for the next step of our research and eight bacteria strains were cultured anaerobically. Seven of them produced 1,3-propanediol with a yield of 0.47-0.58. Six of the the isolated microorganisms were then classified as Clostridium butyricum (four strains), C. lituseburense (one strain), and C. sartagoforme (one strain). We suggest that of all these strains C. butyricum 2CR371.5 is the best 1,3-propanediol producer as producing no lactate as a by-product and growing well on a glycerol-containing medium.
1,3-propanediol production by Escherichia coli expressing genes of dha operon from Clostridium butyricum 2CR371.5.
Authors: Dąbrowski Sławomir , Pietrewicz-Kubicz Dorota , Zabłotna Ewa , Długołęcka Anna
Published: March 2019

1,3-propanediol is used as a monomer in the production of some polymers e.g. polytrimethylene terephthalate used in the production of carpets and textile fibers and in the thermoplastics engineering. However, the traditional chemical synthesis is expensive, generates some toxic intermediates and requires a reduction step under high hydrogen pressure. Biological production of 1,3-propanediol could be an attractive alternative to the traditional chemical methods. Moreover, crude glycerol which is a by-product of biodiesel production, can be used. We constructed a recombinant Escherichia coli strain producing 1,3-propanediol from glycerol by introducing genes of the dha operon from Clostridium butyricum 2CR371.5, a strain from our collection of environmental samples and strains. The E. coli strain produced 3.7 g of 1,3-propanediol per one litre of culture with the yield of 0.3 g per 1 g of glycerol consumed.
PDE4 inhibitors have no effect on eotaxin expression in human primary bronchial epithelial cells.
Authors: Paplińska-Goryca Magdalena , Chazan Ryszarda , Grubek-Jaworska Hanna
Published: March 2019

The bronchial epithelium is a very important factor during the inflammatory response, it produces many key regulators involved in the pathophysiology of asthma and COPD. Local influx of eosinophils, basophils, Th2 lymphocytes and macrophages is the source of many cytotoxic proteins, cytokines and other mediators of inflammation. These cells are attracted by eotaxins (eotaxin-1/CCL11, eotaxin-2/CCL24, eotaxin-3/CCL26). Inhibitors of phosphodiesterase 4 (PDE4) are new anti-inflammatory drugs which cause cAMP accumulation in the cell and inhibit numerous stages of allergic inflammation. The aim of our study was to evaluate the influence of PDE4 inhibitors: rolipram and RO-20-1724 on the expression of eotaxins in human primary bronchial epithelial cells. Cells were preincubated with PDE4 inhibitors for 1 hour and then stimulated with IL-4 or IL-13 alone or in combination with TNF-α. After 48 hours, eotaxin protein level was measured by ELISA and mRNA level by real time PCR. These cells produce CCL24 and CCL26. PDE4 inhibitors increased CCL24 and CCL26 mRNA level irrespectively of the used stimulators. Rolipram and RO-20-1724 had no effect on eotaxin protein production in our experimental conditions. Thus PDE4 inhibitors have no effect on eotaxin protein expression in human primary bronchial epithelial cells. In vitro experiments should be performed using a primary cell model rather than immortalized lines.
An improved method for RNA isolation from plants using commercial extraction kits.
Authors: Kalinowska Elżbieta , Chodorska Maria , Paduch-Cichal Elżbieta , Mroczkowska Karolina
Published: March 2019

Isolation of RNA from plants rich in secondary metabolites using commercial kits often results in contaminated preparations which are not suitable for downstream applications. Although many specific protocols appropriate for plants with a high content of phenolics, anthocyanins and polysaccharides have been developed, these are often expensive, time consuming and not applicable to different types of tissues. This study presents a simple and efficient modification of RNA extraction from different types of tissues using two commercial reagent kits. By simple improvement, we routinely obtained high-quality RNA of the following plants: the blackcurrant bush, black chokeberry bush, pear tree, apricot tree, apple tree, hardy kiwi, tangerine tree, highbush blueberry and cranberry plant.
Bacterial putative metacaspase structure from Geobacter sulfureducens as a template for homology modeling of type II Triticum aestivum metacaspase (TaeMCAII).
Authors: Dudkiewicz Malgorzata Z , Piszczek Ewa
Published: March 2019

Metacaspases, cysteine proteases belonging to the peptidase C14 family, are suspected of being involved in the programmed cell death of plants, although their sequences and substrate specificity differ from those of animal caspases. At present, the knowledge on the metacaspase reaction mechanism is based only on biochemical data and homology models constructed on caspase templates. Here we propose a novel template for metacaspase modeling and demonstrate important advantages in comparison to the conventionally used caspase templates. We also point out the connection between plant and bacterial metacaspases, underlining the prokaryotic roots of Programmed Cell Death (PCD).
A novel homozygous mutation in the WNK1/HSN2 gene causing hereditary sensory neuropathy type 2.
Authors: Potulska-Chromik Anna , Kabzińska Dagmara , Lipowska Marta , Kostera-Pruszczyk Anna , Kochański Andrzej
Published: March 2019

Hereditary sensory and autonomic neuropathy type 2 is a rare disorder caused by recessive mutations in the WNK1/HSN2 gene located on chromosome 12p13.33. Phenotype of the patients is characterized by severe sensory loss affecting all sensory modalities. We report a novel homozygous Lys179fsX182 (HSN2); Lys965fsX968 (WNK1/HSN2) mutation causing an early childhood onset hereditary sensory and autonomic neuropathy type 2, with acromutilations in upper and lower limbs, and autonomic dysfunction. To the best of our knowledge this is the first genetically proven case of hereditary sensory and autonomic neuropathy type 2 originating from East Europe.
Properties of recombinant trehalose synthase from Deinococcus radiodurans expressed in Escherichia coli.
Authors: Filipkowski Paweł , Pietrow Olga , Panek Anna , Synowiecki Józef
Published: March 2019

A trehalose synthase gene from Deinococcus radiodurans (DSMZ 20539) containing 1659 bp reading frame encoding 552 amino acids was amplified using PCR. The gene was finally ligated into pET30Ek/LIC vector and expressed after isopropyl β-d-thiogalactopyranoside induction in Escherichia coli (DE3) Rosetta pLysS. The recombinant trehalose synthase (DraTreS) containing a His(6)-tag at the C-terminus was purified by metal affinity chromatography and characterized. The expressed enzyme is a homodimer with molecular mass of 126.9 kDa and exhibits the highest activity of 11.35 U/mg at pH 7.6 and at 30°C. DraTreS activity was almost unchanged after 2 h preincubation at 45°C and pH 7.6, and retained about 56% of maximal value after 8 h incubation at 50°C. The DraTreS was strongly inhibited by Cu(2+), Hg(2+), Zn(2+), Al(3+) and 10 mM Tris. The K(m) value of maltose conversion was 290.7 mM.
Certain protein transducing agents convert translocated proteins into cell killers.
Authors: Tcherniuk Siergiej , Fiser Anne-Laure , Derouazi Madiha , Toussaint Bertrand , Wang Yan , Wojtal Izabela , Kondo Eisaku , Szolajska Ewa , Chroboczek Jadwiga
Published: March 2019

The majority of proteins are unable to translocate into the cell interior. Hence for peptide- and protein-based therapeutics a direct intracytoplasmic delivery with the aid of transducing agents is an attractive approach. We wanted to deliver to the cell interior a putatively cytotoxic protein VPg. Protein transduction was achieved in vitro with three different commercial products. However, in our hands, delivery of various control proteins without known deleterious effects, as well as of protein VPg, always induced cell death. Finally, we used a novel transducing peptide Wr-T, which was not toxic to cultured cells, even in a quite large range of concentrations. Most importantly, control protein delivered to cells in culture did not display any toxicity while VPg protein exerted a strong cytotoxic effect. These data show that results obtained with cell-penetrating agents should be interpreted with caution.
The "H5N1 publication case" and its conclusions.
Authors: Becker Gregor
Published: March 2019

The request of the National Science Advisory Board for Biosecurity (NSABB) to the editors of the scientific journals SCIENCE and NATURE not to publish details on the modified H5N1-virus has surprisingly not caused a discussion on censorship within the scientific community (NSABB, 2012a, P.1). This may show that science generally acknowledges the necessity to cut out sensitive data from research results in publications that may serve as a manual for weapons of mass destruction. In this article the policy of the NSABB and the reaction of the scientific community is discussed, as well as the meaning of censorship in dual use research and how an appropriate organisation of future surveillance in sensitive science fields could be organised: To guarantee future undisturbed work in sensitive science fields, the establishment of an internationally organised frame for scientists dealing with dual-use-research is suggested.