bluuebunny/crossref_metadata_2025 huggingface dataset:
Different Molecular Forms of TFF3 in the Human Respiratory Tract: Heterodimerization with IgG Fc Binding Protein (FCGBP) and Proteolytic Cleavage in Bronchial Secretions
Authors: Weste Jens , Houben Till , Harder Sönke , Schlüter Hartmut , Lücke Eva , Schreiber Jens , Hoffmann Werner
Published: December 2022

The polypeptide TFF3 belongs to the trefoil factor family (TFF) of lectins. TFF3 is typically secreted from mucous epithelia together with mucins. Both intestinal and salivary TFF3 mainly exist as disulfide-linked heterodimers with IgG Fc binding protein (FCGBP). Here, we investigated bronchial tissue specimens, bronchial secretions, and bronchoalveolar lavage (BAL) fluid from patients with a chronic obstructive pulmonary disease (COPD) background by fast protein liquid chromatography and proteomics. For the first time, we identified different molecular forms of TFF3 in the lung. The high-molecular mass form represents TFF3-FCGBP oligomers, whereas the low-molecular mass forms are homodimeric and monomeric TFF3 with possibly anti-apoptotic activities. In addition, disulfide-linked TFF3 heterodimers with an Mr of about 60k and 30k were detected in both bronchial secretions and BAL fluid. In these liquids, TFF3 is partly N-terminally truncated probably by neutrophil elastase cleavage. TFF3-FCGBP is likely involved in the mucosal innate immune defense against microbial infections. We discuss a hypothetical model how TFF3 might control FCGBP oligomerization. Furthermore, we did not find indications for interactions of TFF3-FCGBP with DMBT1gp340 or the mucin MUC5AC, glycoproteins involved in mucosal innate immunity. Surprisingly, bronchial MUC5AC appeared to be degraded when compared with gastric MUC5AC.
A Microwell-Based Intestinal Organoid-Macrophage Co-Culture System to Study Intestinal Inflammation
Authors: Kakni Panagiota , Truckenmüller Roman , Habibović Pamela , van Griensven Martijn , Giselbrecht Stefan
Published: December 2022

The mammalian intestinal epithelium contains more immune cells than any other tissue, and this is largely because of its constant exposure to pathogens. Macrophages are crucial for maintaining intestinal homeostasis, but they also play a central role in chronic pathologies of the digestive system. We developed a versatile microwell-based intestinal organoid-macrophage co-culture system that enables us to recapitulate features of intestinal inflammation. This microwell-based platform facilitates the controlled positioning of cells in different configurations, continuous in situ monitoring of cell interactions, and high-throughput downstream applications. Using this novel system, we compared the inflammatory response when intestinal organoids were co-cultured with macrophages versus when intestinal organoids were treated with the pro-inflammatory cytokine TNF-α. Furthermore, we demonstrated that the tissue-specific response differs according to the physical distance between the organoids and the macrophages and that the intestinal organoids show an immunomodulatory competence. Our novel microwell-based intestinal organoid model incorporating acellular and cellular components of the immune system can pave the way to unravel unknown mechanisms related to intestinal homeostasis and disorders.
BioMThermDB 1.0: Thermophysical Database of Proteins in Solutions
Authors: Nikolić Mina , Brudar Sandi , Coutsias Evangelos , Dill Ken A. , Lukšič Miha , Simmerling Carlos , Hribar-Lee Barbara
Published: December 2022

We present here a freely available web-based database, called BioMThermDB 1.0, of thermophysical and dynamic properties of various proteins and their aqueous solutions. It contains the hydrodynamic radius, electrophoretic mobility, zeta potential, self-diffusion coefficient, solution viscosity, and cloud-point temperature, as well as the conditions for those determinations and details of the experimental method. It can facilitate the meta-analysis and visualization of data, can enable comparisons, and may be useful for comparing theoretical model predictions with experiments.
Extracellular Acidosis Differentially Regulates Estrogen Receptor β-Dependent EMT Reprogramming in Female and Male Melanoma Cells
Authors: Peppicelli Silvia , Ruzzolini Jessica , Lulli Matteo , Biagioni Alessio , Bianchini Francesca , Caldarella Adele , Nediani Chiara , Andreucci Elena , Calorini Lido
Published: December 2022

Clinical outcomes of melanoma patients pointed out a gender disparity that supports a correlation between sex hormone activity on estrogen receptors (ER) and melanoma development and progression. Here, we found that the epithelial-to-mesenchymal transition (EMT) of melanoma cells induced by extracellular acidosis, which is a crucial hallmark of solid cancers, correlates with the expression of ERβ, the most representative ER on melanoma cells. Extracellular acidosis induces an enhanced expression of ERβ in female cells and EMT markers remain unchanged, while extracellular acidosis did not induce the expression of ERβ in male cells and EMT was strongly promoted. An inverse relationship between ERβ expression and EMT markers in melanoma cells of different sex exposed to extracellular acidosis was revealed by two different technical approaches: florescence-activated cell sorting of high ERβ expressing cell subpopulations and ERβ receptor silencing. Finally, we found that ERβ regulates EMT through NF-κB activation. These results demonstrate that extracellular acidosis drives a differential ERβ regulation in male and female melanoma cells and that this gender disparity might open new perspectives for personalized therapeutic approaches.
Factor XI/XIa Inhibition: The Arsenal in Development for a New Therapeutic Target in Cardio- and Cerebrovascular Disease
Authors: Badimon Juan J. , Escolar Gines , Zafar M. Urooj
Published: December 2022

Despite major advancements in the development of safer and more effective anticoagulant agents, bleeding complications remain a significant concern in the treatment of thromboembolic diseases. Improvements in our understanding of the coagulation pathways highlights the notion that the contact pathway—specifically factor XI (FXI)—has a greater role in the etiopathogenesis of thrombosis than in physiological hemostasis. As a result, a number of drugs targeting FXI are currently in different stages of testing and development. This article aims to review the different strategies directed towards FXI-inhibition with a brief summation of the agents in clinical development, and to comment on the therapeutic areas that could be explored for potential indications. Therapeutics targeting FXI/FXIa inhibition have the potential to usher in a new era of anticoagulation therapy.
A Comparative Study of Urinary Tests and Cultures for the Effectiveness of Fosfomycin in Catheter-Related Urinary Tract Infections
Authors: Jo Jung Ki , Kim Dong Seob , Sim Younghun , Ryu Soorack , Kim Kyu Shik
Published: December 2022

As the elderly population increases due to an aging society, the number of patients with catheters is increasing, and treatment for urinary infections is needed. The current study analyzed the effectiveness of fosfomycin, the primary antibiotic used to treat urinary tract infections (UTIs), in these patients. Patients who received fosfomycin as the primary antibiotic for a UTI were selected, and the results of urine tests and cultures before and after fosfomycin administration were compared and analyzed. The degree of UTI in patients with a catheter was found to be more severe (p = 0.020), and the infecting strains were found to be different depending on whether a catheter was present (p = 0.014). There was a difference in the treatment success rate depending on whether or not a catheter was present (53.6% vs. 70.4%), but it was found that the treatment rate was more than 50% regardless of whether a catheter was present. The bacterial type, as well as the treatment rate based on the bacterium, differed depending on the presence of a catheter. Fosfomycin has a success rate of more than 50%, even in patients with catheters; therefore, it can be considered the primary antibiotic for treating UTIs.
The Relationship between Serum Adiponectin, Urinary Albumin/Creatinine Ratio and Type 2 Diabetes: A Population-Based Cross-Sectional Study
Authors: Ono Shoma , Mizushiri Satoru , Nishiya Yuki , Tamura Ayumi , Hamaura Kiho , Ito Ryoma , Terada Akihide , Tanabe Jutaro , Yanagimachi Miyuki , Wai Kyi Mar , Sawada Kaori , Ihara Kazushige , Daimon Makoto
Published: December 2022

The relationship between serum adiponectin concentration (S-Adipo) and various diseases, such as type 2 diabetes (T2D) is conflicting. We hypothesized that the extent of kidney damage in patients with T2D may be responsible for this inconsistency and, thus, examined association between S-Adipo and T2D after consideration for the extent of kidney damage present. Of the 1816 participants in the population-based Iwaki study of Japanese people, 1751 participants with a complete dataset were included. Multivariate logistic regression analyses revealed that low S-Adipo was independently associated with T2D (<0.001), as was high urinary albumin to creatinine ratio (uACR) (<0.001). Principal components analysis showed that the relative value of S-Adipo to uACR (adiponectin relative excess) was significantly associated with T2D (odds ratio: 0.49, p < 0.001). Receiver operating curve analyses revealed that an index of adiponectin relative excess the ratio of S-Adipo to uACR was superior to S-Adipo per se as a marker of T2D (area under the curve: 0.746 vs. 0.579, p < 0.001). This finding indicates that the relationship between S-Adipo and T2D should be evaluated according to the extent of kidney damage present and may warrant similar analyses of the relationships between S-Adipo and other medicalconditions, such as cardiovascular disease.
Editorial: Recent Advances in Thyroid Surgery
Authors: Scerrino Gregorio , Richiusa Pierina , Graceffa Giuseppa , Lori Eleonora , Sorrenti Salvatore , Paladino Nunzia Cinzia
Published: December 2022

Thyroid surgery has been, since its earliest application, one of the most notable fields in medicine, illustrated by the fact that the Nobel Prize in Medicine was won, for the first time, for thyroid surgery by Emil Theodor Kocher (1841–1917) in 1909, for his contributions to thyroid physiology, pathology, and surgery [...]
Does Obesity Influence Women’s Decision Making about the Mode of Delivery?
Authors: Walędziak Maciej , Różańska-Walędziak Anna
Published: December 2022

Introduction: The ratio of cesarean deliveries (CDs) has been increasing worldwide, with a growing problem of cesarean delivery on maternal request (CDMR) and an alarmingly increasing rate of CD in the private sector. There are numerous factors influencing women’s preferences for the mode of delivery and their opinion about shared decision making (SDM). Material and method: The study was designed as an online survey, filled in by 1040 women. The questionnaire included questions about women’s preferences for the mode of delivery, their opinions about CDMR and the process of decision making regarding the mode of delivery. Results: There were no statistically significant differences found between women with a BMI ≤ 25 kg/m2 and >25 kg/m2, nor with a BMI ≤ 30 kg/m2 and >30 kg/m2, on the subject of the preferred method of delivery, and the opinion regarding SDM and CDMR without medical indications. More than 85% of women in all groups, who preferred CD as the mode of delivery, wanted to have CDMR. Conclusion: We have not found obesity and overweight to be a factor influencing women’s preferred mode of delivery, their opinion about SDM and their preference for CDMR without medical indications. However, the sample size of women with morbid obesity with a BMI ≥ 35 kg/m2 was too small for the results to be considered significant in this group, and it will therefore be subject to further studies.
Quality of Life in Oral Cancer Patients in Greek Clinical Practice: A Cohort Study
Authors: Lavdaniti Maria , Tilaveridis Ioannis , Palitzika Dimitra , Kyrgidis Athanasios , Triaridis Stefanos , Vachtsevanos Konstantinos , Kosintzi Angeliki , Antoniades Konstantinos
Published: December 2022

Cancer of the oral cavity is one of the most common cancers all over the world. Oral cancer and its treatment impacts on patients’ Quality of Life (QOL). The purpose of the present study was to assess oral cancer patients’ QOL after the completion of surgical therapy, and to investigate factors affecting it. This was a prospective cohort study, conducted at the Department of Oral & Maxillofacial Surgery, of a large general public hospital in Northern Greece. The sample consisted of 135 consecutive eligible cancer patients. Three distinct questionnaires were used. The first one included questions regarding the participants’ demographic characteristics and relevant clinical information. The second comprised the European Organization for Research and Treatment core module (EORTC QLQ-C30) and its head and neck module EORTC QLQ-H&N35. The third was the Functional Assessment of Cancer Therapy–General (FACT-G) assessment of quality of life. We also included the physician-completed Karnofsky scale to assess the functional status of the participants. We found that location of the tumor affects QOL and specifically social contact (H = 17.89, p = 0.001), on the first assessment, and nutritional supplements (H = 22.49, p = 0.000), on the fourth assessment. QOL in patients deteriorates immediately after treatment but significantly improves over time. Health care professionals should take into account these results and arrange care plans in order to find ways to increase patients’ QOL.
DNA Barcoding Is a Useful Tool for the Identification of the Family Scaridae in Hainan
Authors: Liu Bo , Yan Yali , Zhang Nan , Guo Huayang , Liu Baosuo , Yang Jingwen , Zhu Kecheng , Zhang Dianchang
Published: December 2022

Species markers can be quickly and accurately assessed using DNA barcoding. We investigated samples from the parrotfish family Scaridae using DNA barcoding in Hainan. A total of 401 DNA barcodes were analyzed, including 51 new barcodes generated from fresh material, based on a 533 bp fragment of the cytochrome c oxidase subunit I (CO I) gene. There were 350 CO I barcode clusters that matched 43 species from the Barcode of Life Data Systems (BOLD) and GenBank databases. The results showed the following average nucleotide compositions for the complete dataset: adenine (A, 22.7%), thymine (T, 29.5%), cytosine (C, 29.5%), and guanine (G, 18.2%). The mean genetic distance between confamilial species was nearly 53-fold greater than that between individuals within the species. In the neighbor-joining tree of CO I sequences, Chlorurus sordidus and C. spilurus clustered together, and all other individuals clustered by species. Our results indicated that DNA barcoding could be used as an effective molecular tool for monitoring, protecting, and managing fisheries, and for elucidating taxonomic problem areas that require further investigation.
Impact Mechanism of the Three Pilot Reforms of the Rural Land System on Rural Residential Land Use Transition: A Regime Shifts Perspective
Authors: Shu Bangrong , Qu Yi
Published: December 2022

Understanding the impact of the three pilot reforms of the rural land system (TRRLS) on rural residential land use transition (RRLUT) based on the land use transition (LUT) theory is crucial for promoting rural land system reform. However, there is still a lack of research on this, and the LUT theory also needs to be improved from a systematic perspective to eliminate the misunderstanding of LUT in academia. To address this, this study firstly attempts to deepen the conceptual model and the understanding of characteristics of LUT from a regime shifts perspective. LUT is the transformation of the land use system as one regime passes into another, where a difference in the analytical perspective of land use morphology generates different transition results. The process of LUT can simultaneously or solely involve dominant morphology and recessive morphology transitions, and there are two types of LUT: positive and negative transitions. Moreover, LUT in different regions may have pathway differences and the convergence of results. Then, a theoretical analysis framework of the pathways of RRLUT under the TRRLS is constructed to detect the impact mechanism by using Wujin district, China to obtain empirical evidence. The results reveal that the recessive morphology transition of rural residential land in Wujin under the TRRLS is significant, while the dominant morphology transition in land quantity structure and spatial distribution is relatively slow. Furthermore, two internal factors of population urbanization and migration, the demand for rural collective economic development, as well as the two external factors of the TRRLS and market factors, such as nonlocals’ demand for housing and rural enterprises’ demand for land, have, to a certain extent, weakened the resilience of the rural land use system and promoted RRLUT. Here, the TRRLS have, by removing the institutional barrier to RRLUT, become the key to the transition.
Phrase-Level ATR Vowel Harmony in Anum—A Case of Recursive Prosodic Phrasing
Authors: Kügler Frank
Published: December 2022

(1) Like many other Kwa languages, Anum employs a pattern of [ATR] vowel harmony that is regressive and [+ATR] dominant (RVH). This paper analyses RVH as a phrasal process which takes into account recursive phonological phrases. The proposal argues for an application of the process within and across non-maximal phonological phrases (φ) and a blocking of application across maximal phonological phrases (φmax). (2) Investigating RVH in Anum in more detail, the size of constituents and the complexity of sentence structures are varied. Target sentences were recorded and transcribed for [ATR] vowel harmony. (3) The empirical data show that RVH applies frequently between words that belong to either the same or to different syntactic constituents, but is blocked between two verb phrases of a serial verb construction and between any word and a following sentence-final time adverbial. Interestingly, RVH occurs between a sentence-initial subject constituent and a following verb or verb phrase, independent of the size of the subject constituent and the remaining number of words in the sentence. (4) The proposed OT analysis accounts for RVH within syntax-phonology Match Theory and addresses both word-level and phrase-level harmony. The special behaviour of subject constituents that prosodically phrase together with verbs and with constituents of the verb phrase (VP) is discussed. Either a phonological well-formedness constraint or a syntactically distinct input may account for phrasing effects with subject constituents in Anum.
Infrared Spectroscopic Verification of a α-Helical Collagen Structure in Glutaraldehyde-Free Crosslinked Bovine Pericardium for Cardiac Implants
Authors: Welzel Cindy , König Ulla , Jannasch Anett , Matschke Klaus , Tugtekin Sems-Malte , Dittfeld Claudia , Steiner Gerald
Published: December 2022

The degeneration of heart valve bioprostheses due to calcification processes is caused by the intercalation of calciumhydroxyapatite in pericardium collagen bundles. Variations of the protein secondary structure of biomaterials according to preparation are relevant for this mineralization process and thus the structural characterization of innovative bioprostheses materials is of great importance. The gold standard for prostheses preparation is glutaraldehyde (GA)-fixation of bovine pericardium that adversely promotes calcification. The novel GA-free SULEEI-treatment of bovine pericardium includes decellularization, UV-crosslinking, and electron beam sterilization. The aim of this study is the structural characterization of SULEEI-treated and GA-fixed bovine pericardium. IR spectroscopic imaging combined with multivariate data and curve fit analysis was applied to investigate the amide I and amide II regions of SULEEI-treated and GA-fixed samples. The spectroscopic images of GA-fixed pericardial tissue exhibited a generally high content of amine groups and side chains providing nucleation points for calcification processes. In contrast, in SULEEI-treated tissue, the typical α-helical structure was retained and was supposed to be less prone to deterioration.
Experimental Study on the Lubrication Enhancement of Slider-on-Disc Contact by Stearic Acid Adsorption under Limited Lubricant Supply
Authors: Jian Yusheng , Jing Zhaogang , Guo Feng , Wong Pat Lam , Li Xinming
Published: December 2022

The optimization of the lubricant supply quantity contributes to minimizing energy losses and wastage. To enhance the performance of hydrodynamic bearings running with limited lubricant supply (LLS), this study examined the effect of stearic acid as an additive. Stearic acid is commonly used for boundary lubrication as an organic friction modifier. How the stearic acid adsorption affects the hydrodynamic lubrication of a slider-on-disc contact under LLS was investigated using interferometry measurement and fluorescence observation in this study. Firstly, the oil reservoir of PAO10 with stearic acid adsorption was observed at the slider entrance. Secondly, the film thickness versus speed of PAO10 and PAO10 with 0.1% and 0.3% stearic acid, respectively, were measured. Finally, the morphology and surface properties of the glass and steel blocks with stearic acid adsorption were characterized by atomic force microscopy and Fourier transform infrared spectroscopy. The results show that the stearic acid adsorption layer weakens the wettability of the lubrication track and induces the ‘dewetting’ phenomenon of the lubricating oil. Thus, discrete oil distribution in the form of stripe or droplet can be generated, which leads to the accumulation of lubricating oil at the slider entrance to form a reservoir. An additional inlet pressure that is generated by the oil reservoir due to surface tension increases the oil film formation capacity. Furthermore, the morphologies of the adsorbed stearic acid layer on the glass and the steel blocks are, respectively, characterized by the nano-sized granular bulge and brush structure. This study reveals a new role of stearic acid adsorption in promoting LLS lubrication.
Precipitation Behavior during Aging Operations in an Ultrafine-Grained Al–Cu–Mg Alloy Produced by High-Strain-Rate Processing
Authors: Zhang Linyan , Luo Hongyun
Published: December 2022

An ultrafine-grained (UFG) Al–Cu–Mg alloy (AA2024) was produced by surface mechanical grinding treatment (SMGT) with a high strain rate, and the precipitation behavior inside the grain and at the grain boundary was investigated. During SMGT, element segregation at the boundary was rarely observed, since the solute atoms were impeded by dislocations produced during SMGT. During early aging, the atomic fraction of Cu at the grain boundary with SMGT alloys was approximately 2.4-fold larger than that without SMGT alloys, the diffusion rate of Cu atoms from the grain toward the grain boundaries was accelerated with SMGT alloys, because a higher local elastic stress and diffusion path were provided by high-density dislocations. The combined action, in terms of the composition of the alloy, the atomic radius, the diffusion path, and the diffusion driving force provided by high-density dislocations with SMGT alloys, led to a Cu/Mg atomic ratio of approximately 6.8 at the grain boundary. The average size of the precipitates inside the grain was approximately 2- and 10-fold larger than that formed after later aging with and without SMGT alloys, due to more nucleation sites at dislocation located inside the grain with SMGT alloys having attracted and captured numerous solute atoms during the aging process.
Reliability Analysis of the Multi-State k-out-of-n: F Systems with Multiple Operation Mechanisms
Authors: Song Yanbo , Wang Xiaoyue
Published: December 2022

Modern engineering systems are designed and utilized to realize complicated functions, and their operation mechanisms are becoming more complex. Nevertheless, prior related research mainly focused on the reliability evaluations of the systems with a single operation mechanism, which are not appropriate to depict the operation process of systems with multiple operation mechanisms. Faced with the research gaps and practical needs, this paper establishes a new reliability model for the multi-state k-out-of-n: F system composed of n subsystems, which runs under multiple interactive operation mechanisms, including performance sharing, balanced requirement, and protection strategy. The units in each subsystem can share the performance via a common bus, with the purpose of regulating the performance of all equal units. A new triggering criterion of the protection device in each subsystem is proposed based on the total performance of the units. Due to the protection from the device, the degradation rate of the units between two adjacent states decreases to a lower rate. Each subsystem breaks down when the total performance of the units reaches a critical value. According to the number of failed subsystems, the state of the entire system can be divided into multiple states. The Markov process imbedding method combined with the finite Markov chain imbedding approach is developed to obtain the probabilistic indexes of each subsystem and the entire system. The applicability of the proposed model and the effectiveness of the method can be sufficiently demonstrated by illustrative examples and sensitivity analyses.
MACA: Multi-Agent with Credit Assignment for Computation Offloading in Smart Parks Monitoring
Authors: She Liang , Wang Jianyuan , Bo Yifan , Zeng Yangyan
Published: December 2022

Video monitoring has a wide range of applications in a variety of scenarios, especially in smart parks. How to improve the efficiency of video data processing and reduce resource consumption have become of increasing concern. The high complexity of traditional computation offloading algorithms makes it difficult to apply them to real-time decision-making scenarios. Thus, we propose a multi-agent deep reinforcement learning algorithm with credit assignment (MACA) for computation offloading in smart park monitoring. By making online decisions after offline training, the agent can give consideration to both decision time and accuracy in effectively solving the problem of the curse of dimensionality. Via simulation, we compare the performance of MACA with traditional deep Q-network reinforcement learning algorithm and other methods. Our results show that MACA performs better in scenarios where there are a higher number of agents and can minimize request delay and reduce task energy consumption. In addition, we also provide results from a generalization capability verified experiment and ablation study, which demonstrate the contribution of MACA algorithm to each component.
Effect of Atmospheric Conditions on Pathogenic Phenotypes of Arcobacter butzleri
Authors: Martins Rodrigo , Mateus Cristiana , Domingues Fernanda , Bücker Roland , Oleastro Mónica , Ferreira Susana
Published: December 2022

Arcobacter butzleri is an emergent gram-negative enteropathogenic bacterium widespread in different environments and hosts. During the colonization of the gastrointestinal tract, bacteria face a variety of environmental conditions to successfully establish infection in a new host. One of these challenges is the fluctuation of oxygen concentrations encountered not only throughout the host gastrointestinal tract and defences but also in the food industry. Oxygen fluctuations can lead to modulations in the virulence of the bacterium and possibly increase its pathogenic potential. In this sense, eight human isolates of A. butzleri were studied to evaluate the effects of microaerobic and aerobic atmospheric conditions in stressful host conditions, such as oxidative stress, acid survival, and human serum survival. In addition, the effects on the modulation of virulence traits, such as haemolytic activity, bacterial motility, biofilm formation ability, and adhesion and invasion of the Caco-2 cell line, were also investigated. Overall, aerobic conditions negatively affected the susceptibility to oxygen reactive species and biofilm formation ability but improved the isolates’ haemolytic ability and motility while other traits showed an isolate-dependent response. In summary, this work demonstrates for the first time that oxygen levels can modulate the potential pathogenicity of A. butzleri, although the response to stressful conditions was very heterogeneous among different strains.
Manipulation of Whitefly Behavior by Plant Viruses
Authors: Zhao Kai , Liu Shu-Sheng , Wang Xiao-Wei , Yang Jin-Guang , Pan Li-Long
Published: December 2022

Whiteflies of the Bemisia tabaci complex transmit hundreds of plant viruses belonging to the genera Begomovirus and Crinivirus, among others. Tripartite interactions of whitefly–virus–plant frequently occur during virus infection and transmission. Specifically, virus transmission-related behavior of whitefly, such as preference and feeding, may be altered by viruses and thus exert significant impacts on the outcome of virus spread and epidemics. Here, we provide an overview on the current understanding of the manipulation of whitefly behavior by plant viruses. Plant viruses can significantly modulate whitefly preference and feeding behavior, either directly or in a plant-mediated manner. In general, non-viruliferous whiteflies tend to prefer virus-infected plants, and viruliferous whiteflies are more likely to prefer uninfected plants. In most cases, virus infection of plants and/or whitefly seems to exhibit positive or no effects on whitefly feeding on plants. The significance and evolution of these patterns are then discussed. Finally, we suggest several future directions of research, such as the exploration of temporal dynamics and the dissection of underlying mechanisms of virus-induced changes in whitefly behavior.
Manganese Pollution and Its Remediation: A Review of Biological Removal and Promising Combination Strategies
Authors: Wu Rongrong , Yao Fangting , Li Xiaoya , Shi Chongjing , Zang Xue , Shu Xiao , Liu Hengwei , Zhang Wenchao
Published: December 2022

Manganese (Mn), as a cofactor of multiple enzymes, exhibits great significance to the human body, plants and animals. It is also a critical raw material and alloying element. However, extensive employment for industrial purposes leads to its excessive emission into the environment and turns into a significant threat to the ecosystem and public health. This review firstly introduces the essentiality, toxicity and regulation of Mn. Several traditional physicochemical methods and their problems are briefly discussed as well. Biological remediation, especially microorganism-mediated strategies, is a potential alternative for remediating Mn-polluted environments in a cost-efficient and eco-friendly manner. Among them, microbially induced carbonate precipitation (MICP), biosorption, bioaccumulation, bio-oxidation are discussed in detail, including their mechanisms, pivotal influencing factors along with strengths and limitations. In order to promote bioremediation efficiency, the combination of different techniques is preferable, and their research progress is also summarized. Finally, we propose the future directions of Mn bioremediation by microbes. Conclusively, this review provides a scientific basis for the microbial remediation performance for Mn pollution and guides the development of a comprehensive competent strategy towards practical Mn remediation.
Toxicological and Safety Pharmacological Profiling of the Anti-Infective and Anti-Inflammatory Peptide Pep19-2.5
Authors: Möller Clemens , Heinbockel Lena , Garidel Patrick , Gutsmann Thomas , Mauss Karl , Weindl Günther , Fukuoka Satoshi , Loser Dominik , Danker Timm , Brandenburg Klaus
Published: December 2022

Aspidasept (Pep19-2.5) and its derivative Pep19-4LF (“Aspidasept II”) are anti-infective and anti-inflammatory synthetic polypeptides currently in development for application against a variety of moderate to severe bacterial infections that could lead to systemic inflammation, as in the case of severe sepsis and septic shock, as well as application to non-systemic diseases in the case of skin and soft tissue infections (SSTI). In the present study, Aspidasept and Aspidasept II and their part structures were analysed with respect to their toxic behavior in different established models against a variety of relevant cells, and in electrophysiological experiments targeting the hERG channel according to ICH S7B. Furthermore, the effects in mouse models of neurobiological behavior and the local lymph node according to OECD test guideline 429 were investigated, as well as a rat model of repeated dose toxicology according to ICH M3. The data provide conclusive information about potential toxic effects, thus specifying a therapeutic window for the application of the peptides. Therefore, these data allow us to define Aspidasept concentrations for their use in clinical studies as parenteral application.
Comparative Penicillium spp. Transcriptomics: Conserved Pathways and Processes Revealed in Ungerminated Conidia and during Postharvest Apple Fruit Decay
Authors: Bartholomew Holly P. , Lichtner Franz J. , Bradshaw Michael , Gaskins Verneta L. , Fonseca Jorge M. , Bennett Joan W. , Jurick Wayne M.
Published: December 2022

Blue mold, caused by Penicillium spp., is an impactful postharvest disease resulting in significant economic losses due to reduced pome fruit quality and mycotoxin contamination. Using two Penicillium species with different levels of aggressiveness, transcriptomics were implemented in order to identify genes expressed during apple fruit decay and loci expressed in ungerminated conidia. Total RNA was isolated from ungerminated conidia and decayed apple fruit infected with P. expansum R19 or P. polonicum RS1. There were 2442 differentially expressed genes (DEGs) between the R19 and RS1 in apple. Comparisons within species between apple and conidia revealed 4404 DEGs for R19 and 2935 for RS1, respectively. Gene ontology (GO) analysis revealed differential regulation in fungal transport and metabolism genes during decay, suggesting a flux in nutrient acquisition and detoxification strategies. In R19, the oxidoreductase GO category comprised 20% of all DEG groups in apple verses conidia. Ungerminated conidia from both species showed DEGs encoding the glyoxylate shunt and beta-oxidation, specifying the earliest metabolic requirements for germination. This is the first study to identify pre-loaded transcripts in conidia from blue mold fungi, reveal unique genes between species expressed during apple decay, and show the expression dynamics of known fungal virulence factors. These findings will enable development of targeted approaches for blue mold abatement strategies.
Ciliate Microtubule Diversities: Insights from the EFBTU3 Tubulin in the Antarctic Ciliate Euplotes focardii
Authors: Pucciarelli Sandra , Sparvoli Daniela , Ballarini Patrizia , Piersanti Angela , Mozzicafreddo Matteo , Arregui Lucia , Miceli Cristina
Published: December 2022

Protozoans of the Phylum Ciliophora (ciliates) assemble many diverse microtubular structures in a single cell throughout the life cycle, a feature that made them useful models to study microtubule complexity and the role of tubulin isotypes. In the Antarctic ciliate Euplotes focardii we identified five β-tubulin isotypes by genome sequencing, named EFBTU1, EFBTU2, EFBTU3, EFBTU4 and EFBTU5. By using polyclonal antibodies directed against EFBTU2/EFBTU1 and EFBTU3, we show that the former isotypes appear to be involved in the formation of all microtubular structures and are particularly abundant in cilia, whereas the latter specifically localizes at the bases of cilia. By RNA interference (RNAi) technology, we silenced the EFBTU3 gene and provided evidence that this isotype has a relevant role in cilia regeneration upon deciliation and in cell division. These results support the long-standing concept that tubulin isotypes possess functional specificity in building diverse microtubular structures.
Association of Food Intake with Sleep Durations in Adolescents from a Capital City in Northeastern Brazil
Authors: da Silva Emanuellen Coelho , Carneiro Juliana Ramos , de Almeida Fonseca Viola Poliana Cristina , Confortin Susana Cararo , da Silva Antônio Augusto Moura
Published: December 2022

(1) Background: During adolescence, there are significant changes in food consumption, such as reducing the consumption of in natura or minimally processed foods and increasing the consumption of ultra-processed foods. Thus, eating habits can influence sleep duration and, consequently, affect the quality of life of young people. This study thus aims to estimate the association of consumption of in natura or minimally processed, processed, and ultra-processed foods with sleep durations in adolescents. (2) Methods: This is a cross-sectional study including 964 adolescents (18 to 19 years old) from the 1997 to 1998 birth cohort in São Luís, Maranhão. Food consumption was assessed using the food frequency questionnaire (FFQ) and stratified based on the NOVA classification. Sleep duration was verified using accelerometry in hours. The analysis of the association between the consumption of in natura or minimally processed, processedand ultra-processed foods with sleep durations in adolescents used crude and adjusted linear regression (by gender, age, skin color, education, economic class, work, consumption of alcohol, smoking, screen time, physical activity, use of illicit drugs, anxiety, depressive symptoms, and lean and fat mass). A directed acyclic graph (DAG) was used to determine the minimum set of adjustment factors. (3) Results: Of the 964 individuals evaluated, 52.0% were female. The mean sleep duration was 6 h (± 0.95). In the crude and adjusted analyses, no association was observed between food consumption according to the degree of processing and adolescent sleep durations. (4) Conclusion: There was no association between the consumption of in natura or minimally processed, processed, and ultra-processed foods with sleep durations.
Evaluation of a Dietary Supplementation Combining Protein and a Pomegranate Extract in Older People: A Safety Study
Authors: Dormal Valérie , Pachikian Barbara , Debock Elena , Buchet Marine , Copine Sylvie , Deldicque Louise
Published: December 2022

Malnutrition is a highly prevalent condition in older adults. It is associated with low muscle mass and function and increased occurrence of health problems. Maintaining an adequate nutritional status as well as a sufficient nutrient intake in older people is therefore essential to address this public health problem. For this purpose, protein supplementation is known to prevent the loss of muscle mass during aging, and the consumption of various pomegranate extracts induces numerous health benefits, mainly through their antioxidant properties. However, to our knowledge, no study has to date investigated the impact of their combination on the level of malnutrition in older people. The objective of this preliminary study was thus to evaluate the safety of a combination of protein and a pomegranate extract in healthy subjects aged 65 years or more during a 21-day supplementation period. Thirty older participants were randomly assigned to receive protein and a pomegranate extract (Test group) or protein and maltodextrin (Control group) during a 21-day intervention period. The primary outcomes were the safety and tolerability of the supplementation defined as the occurrence of adverse events, and additional secondary outcomes included physical examination and hematological and biochemical parameters. No serious adverse events were reported in any group. Changes in physical, hematological, and biochemical parameters between the initial screening and the end of the study were equivalent in both groups, except for glutamate-pyruvate transaminase (GPT) and prealbumin, for which a decrease was observed only in the Test group. Our initial findings support the safety of the combination of protein and a pomegranate extract in healthy elderly people. Future clinical trials on a larger sample and a longer period are needed to determine the efficacy of this combination.
Mycoplasma ovipneumoniae: A Most Variable Pathogen
Authors: Maksimović Zinka , Rifatbegović Maid , Loria Guido Ruggero , Nicholas Robin A. J.
Published: December 2022

Mycoplasma ovipneumoniae, a well-established respiratory pathogen of sheep and goats, has gained increased importance recently because of its detection in wild ruminants including members of the Cervidae family. Despite its frequent isolation from apparently healthy animals, it is responsible for outbreaks of severe respiratory disease which are often linked to infections with multiple heterologous strains. Furthermore, M. ovipneumoniae is characterized by an unusually wide host range, a high degree of phenotypic, biochemical, and genomic heterogeneity, and variable and limited growth in mycoplasma media. A number of mechanisms have been proposed for its pathogenicity, including the production of hydrogen peroxide, reactive oxygen species production, and toxins. It shows wide metabolic activity in vitro, being able to utilize substrates such as glucose, pyruvate, and isopropanol; these patterns can be used to differentiate strains. Treatment of infections in the field is complicated by large variations in the susceptibility of strains to antimicrobials, with many showing high minimum inhibitory concentrations. The lack of commercially available vaccines is probably due to the high cost of developing vaccines for diseases in small ruminants not presently seen as high priority. Multiple strains found in affected sheep and goats may also hamper the development of effective vaccines. This review summarizes the current knowledge and identifies gaps in research on M. ovipneumoniae, including its epidemiology in sheep and goats, pathology and clinical presentation, infection in wild ruminants, virulence factors, metabolism, comparative genomics, genotypic variability, phenotypic variability, evolutionary mechanisms, isolation and culture, detection and identification, antimicrobial susceptibility, variations in antimicrobial susceptibility profiles, vaccines, and control.
Host tRNA-Derived RNAs Target the 3′Untranslated Region of SARS-CoV-2
Authors: Hendrickson Emily N. , Ericson Marna E. , Bemis Lynne T.
Published: December 2022

The COVID-19 pandemic revealed a need for new understanding of the mechanisms regulating host–pathogen interactions during viral infection. Transfer RNA-derived RNAs (tDRs), previously called transfer RNA fragments (tRFs), have recently emerged as potential regulators of viral pathogenesis. Many predictive studies using bioinformatic approaches have been conducted providing a repertoire of potential small RNA candidates for further analyses; however, few targets have been validated to directly bind to SARS-CoV-2 sequences. In this study, we used available data sets to identify host tDR expression altered in response to SARS-CoV-2 infection. RNA-interaction-prediction tools were used to identify sequences in the SARS-CoV-2 genome where tDRs could potentially bind. We then developed luciferase assays to confirm direct regulation through a predicted region of SARS-CoV-2 by tDRs. We found that two tDRs were downregulated in both clinical and in vitro cell culture studies of SARS-CoV-2 infection. Binding sites for these two tDRs were present in the 3′ untranslated region (3′UTR) of the SARS-CoV-2 reference virus and both sites were altered in Variants of Concern (VOCs) that emerged later in the pandemic. These studies directly confirm the binding of human tDRs to a specific region of the 3′UTR of SARS-CoV-2 providing evidence for a novel mechanism for host–pathogen regulation.
Cytotoxic Labdane Diterpenes, Norlabdane Diterpenes and Bis-Labdanic Diterpenes from the Zingiberaceae: A Systematic Review
Authors: Voon Kelvin Jianmin , Sivasothy Yasodha , Sundralingam Usha , Lalmahomed Aicha , Goh Asly Poh-Tze
Published: December 2022

Over the years, labdane diterpenes, norlabdane diterpenes, and bis-labdanic diterpenes with cytotoxic activities have been identified across various families in the plant kingdom including the Zingiberaceae. The present review discusses the distribution of these labdane-type diterpenes within the Zingiberaceae; their extraction, isolation, and characterization from the respective Zingiberaceae species; the structural similarities and differences within each group and between the different groups of the labdane-type diterpenes; and their cytotoxic activities against breast, cervical, liver, colorectal, pancreatic, lung and prostate cancer cell lines. The review will also provide insight into how the cytotoxic activities of the labdane-type diterpenes are influenced by their structural features.
MdWRKY120 Enhance Apple Susceptibility to Alternaria alternata
Authors: Liu Lifu , Li Xiaoming , Guo Wei , Shi Jiajun , Chen Wenjun , Lei Yingying , Ma Yue , Dai Hongyan
Published: December 2022

Alternaria alternata (A. alternata) is a common pathogen that greatly influences apples’ quantity and quality. However, chemical treatments produce increased health risks along with decreased food and environmental safety. Advancements in plant molecular biology, such as transgenic technology, have increased apple trees’ resistance to pathogens and have therefore attracted widespread attention. WRKY transcription factors are involved in abiotic and biotic stress regulation; however, their biological role in non-model plants such as apple, is still unknown. In this investigation, MdWRKY120 was isolated from the ‘GL-3′ apple to determine its function during Alternaria alternate infection. The MdWRKY120-GFP fusion protein was located in the nucleus. MdWRKY120 in yeast cells exhibited activating transcriptional activity, meaning it is a transcription activator. MdWRKY120 overexpression transgenic plants were more sensitive to A. alternata, while RNAi transgenic plants showed increased resistance to A. alternata. This investigation demonstrates that MdWRKY120 enhances the susceptibility of apples to A. alternata.