bluuebunny/crossref_metadata_2025 huggingface dataset:
Immune-related gene expansions underlie an evolutionary history of viral interactions in the largemouth bass (Micropterus salmoides) genome
Authors: He Kuo , Zhao LiuLan , Yuan Zihao , Canário Adelino , Liu Qiao , Chen Shi-Yi , Guo Jiazhong , Luo Wei , Yan Haoxiao , Zhang Dongmei , Li Lisen , Yang Song
Published: January 2024

Largemouth bass (Micropterus salmoides) has become a cosmopolitan
species due to its widespread introduction as game and domesticated
fish. Here a high-quality chromosome-level reference genome of a female
M. salmoides was produced by combining Illumina, PacBio and Hi-C
sequencing, aiming to better understand the evolution of traits such as
pathogen resistance and environmental adaptations. The genome size was
844.88 Mb with a contig N50 of 15.68 Mb, and 23 chromosomes anchoring
99.9% of the genome sequences. A total of 26,370 protein-coding genes
from 3415 gene families, of which 127 unique to M. salmoides. Mining of
publicly available RNA-Seq sequences from healthy M salmoides identified
sequences of known fish viral pathogens whose apparent tolerance could
be linked to the expansion of 91 immune-related gene families providing
high resistance to disease. The prevalence of such expressed viral
elements captured in the M. salmoides transcriptome suggests an history
of interaction between the fish and the viral environment. The
high-quality genome will be a fundamental resource to understand M.
salmoides traits that enable the successful colonization of novel and
changing environments across the world.
Hybrid modified percutaneous dilatational tracheostomy reduces the risk of COVID-19 aerosol dissemination to healthcare workers; a prospective single centre study
Authors: Choi Yeon Soo , Kim Min Ji , Kim Ji Won
Published: January 2024

Background Conventional tracheostomy and percutaneous dilatational
tracheostomy (PDT) present risks of exposing health care workers to
coronavirus disease (COVID‐19). We report a hybrid modified percutaneous
dilatational tracheostomy (hybrid MPDT) to reduce this risk of COVID-19
aerosol transmission. Methods We performed tracheostomy using a hybrid
MPDT involving a combination of conventional tracheostomy and PDT.
Hybrid MPDT requires a small incision and minimal dissection like
conventional tracheostomy, followed by PDT without bronchoscope guidance
and the use of an endotracheal tube cuffed at cricoid. Results Hybrid
MPDT was successfully performed in 20 patients with COVID‐19. The
infection rate to medical staff was 0% and the average operation time
was 6.45±1.02 min. Conclusion Hybrid MPDT may ensure rapid and safe
airway management in critically ill COVID-19 patients.
Effect of pollution tax on the persistence of a single species in polluted environment
Authors: CARRASCO Carlos RAMIREZ
Published: January 2024

In this paper, we propose a non-autonomous mathematical model of a
single species that is exposed to an environment subject to a pollution
tax when the emission of the toxicant exceeds a limit up to which there
is no harm to the population. We assume that all emissions exceed the
allowable limit and consider the negative effect of tax avoidance.
Furthermore, we assume that a born organism carries with it an amount of
internal toxicant, and that the amount of toxicant stored in living
organisms that die drifts into the environment. We obtain sufficient
parametric conditions to guarantee the persistence of the population and
also note that the tax coefficient is the key parameter that must be
chosen very carefully to maintain the equilibrium level of the
population. Finally, for the initial validity of our results we perform
some numerical simulations.
The left ‘coronary artery sandwich anomaly’ with cardiogenic syncope: a case report.
Authors: Zhu Ye , chen Yinhua , You Jia , Xu Chao , Chen Yong , Gu Xiang
Published: January 2024

Anomalous origin of coronary arteries is usually asymptomatic and a rare
disease. However, some cases can cause severe life-threatening events
such as myocardial infarction and syncope. Management of coronary
anomalies continues to be a controversial topic in clinic, for which
requires a high index of suspicion for timely diagnosis and management.
We report a case of a 15-year-old symptomatic boy who presented with
cardiogenic syncope and was diagnosed with a rare congenital “coronary
artery sandwich anomaly” (CASA), acute subendocardial myocardial
infarction and cardiac failure requiring extracorporeal membrane
oxygenation(ECMO) for stabilization. Speckle tracking echocardiography
examination showed decreased segmental ventricular wall motion and the
left ventricular global longitudinal strain was -9.3%. Cardiac computed
tomography (CT) showed that an anomalous origin of the left coronary
artery arose from the right coronary sinus, with acute angle takeoff and
inter-arterial course between aorta and pulmonary artery. Cardiac
Magnetic Resonance Imaging(MRI) image showed: diffuse abnormal intima
enhancement in the left ventricle, the left ventricle was enlarged and
systolic function was decreased. The best treatment was coronary artery
unroofing operation. However, the patient was with poor cardiac function
and was managed with conservative treatment instead of operation. This
case demonstrated that the left CASA with acute angle takeoff and
inter-arterial course between the aorta and pulmonary artery is
extremely rare but severe potentially life-threatening presentation
which should be managed with a promptly and careful treatment. In this
case, speckle tracking echocardiography, cardiac CT and MRI have played
important roles in diagnosis and therapy of the disease.
Relationships among rhizosphere microbial co-occurrence patterns, core microbiota, and soil multinutrient cycling in Robinia pseudoacacia plantations of different ages
Authors: Jiajia Li , Yang Le , Fan Miaochun , Shangguan Zhouping
Published: January 2024

Vegetation restoration alters soil biotic and abiotic factors. Plants
have evolved multiple strategies to adapt to nutrient limitation by
reshaping and recruiting nutrient cycling-associated microbial
communities in the rhizosphere. However, our understanding of the
co-occurrence patterns of rhizosphere microbial communities and their
role in soil multinutrient cycling during vegetation restoration remains
limited. The present study explored the co-occurrence patterns of
rhizosphere microbial communities along a chronosequence (15–45 years
old) in Robinia pseudoacacia plantations in China’s Loess Plateau
region, the associations between core microbiota and multinutrient
cycling under spatiotemporal changes. Our results indicate that soil
multifunctionality influences microbial co-occurrence patterns in the R.
pseudoacacia rhizosphere, resulting in a more stable bacterial network
than fungal network, forest age was a major driver of modularized
distribution of nodes in bacterial and fungal networks, and forest
age-sensitive microbes were taxonomically diverse at the phylum level.
Meanwhile, we found that core microbiota play essential role in
rhizosphere soil multinutrient cycling under R. pseudoacacia
afforestation.
Do root-sprouting and non-root-sprouting species differ in response to injury? Effect on growth, root buds and phytohormones
Authors: Martínková Jana , Motyka Vaclav , Bitomsky Martin , Adamec Lubomir , Dobrev Petre , Filartiga Arinawa , Filepova Roberta , Gaudinova Alena , Lacek Jozef , Klimesova Jitka
Published: January 2024

Root-sprouting (RS) clonal herbs are reported to be better adapted to
severe disturbance than other clonal species, and their investments in
clonal and storage organs are smaller than those in rhizomes. RS ability
seems advantageous, so why is it not more common among plants? In a pot
greenhouse experiment, we subjected two closely related clonal herbs
differing in RS ability (RS Inula britannica and rhizomatous I.
salicina) to severe biomass removal and looked for potential barriers to
RS. We confirmed RS only in the already reported RS species I.
britannica. However, RS was not boosted by disturbance in this RS
species, i.e., the number of root buds and sprouts was not affected by
biomass removal. Aerobic root respiration did not differ between the RS
and non-RS species, and the phytohormone profiles differed significantly
more between the RS and non-RS species than between the injured and
non-injured individuals. The common hypothesis, however, never tested,
that RS is facilitated by a low auxins to cytokinins content ratio was
supported. Our results suggest that intrinsic phytohormone regulation is
behind RS ability. Injury-causing phytohormonal imbalance seems to be
less important, at least in spontaneously RS species such as I.
britannica.
Inverse scattering transforms of the inhomogeneous fifth-order defocusing nonlinear Schrodinger equation with zero boundary conditions and nonzero boundary conditions: bound-state solitons and rogue waves
Authors: Mao Jinjin , Tian Shoufu , Xu Tian Zhou , Shi Lin-Fei
Published: January 2024

The present work studies the inverse scattering transformation (IST) of
the inhomogeneous fifth-order defocusing nonlinear Schrodinger (ifoNLS)
equation with zero boundary conditions (ZBCs) and non-zero boundary
conditions (NZBCs). Firstly, the bound-state (BS) solitons of the ifoNLS
equation with ZBCs are derived by generalization of the residue theorem
and the Laurent’s series for the first time. Then combining with the
robust IST, the matrix Riemann-Hilbert (RH) problem of the ifoNLS
equation with NZBCs are revealed. Based on the resulting RH problem, a
new higher-order rogue wave (RW) solution of the ifoNLS equation are
found by the modified Darboux transformation. Finally, some
corresponding graphs are given by selecting appropriate parameters to
further discuss the unreported dynamic behavior of the BS solitons and
RW solutions, which have not been reported before.
Whole-genomes from the extinct Xerces Blue butterfly reveal low diversity and long-term population decline
Authors: Lalueza-Fox Carles , de-Dios Toni , Fontsere Claudia , Renom Pere , Stiller Josefin , Llovera Laia , Uliano-Silva Marcela , Wright Charlotte , Lizano Esther , Navarro Arcadi , Robbins Robert , Blaxter Mark , Marquès-Bonet Tomas , Vila Roger
Published: January 2024

The Xerces Blue (Glaucopsyche xerces) is considered to be the first
butterfly to become extinct at global scale in historical times. It was
notable for its chalky lavender wings with conspicuous white spots on
the ventral wings. The last individuals were collected in their
restricted habitat, in the dunes near the Presidio military base in San
Francisco, in 1941. To explore the demographic history of this iconic
butterfly and to better understand why it went extinct, we sequenced at
medium coverage the genomes of four 80 to 100-year-old Xerces Blue
specimens and seven historic specimens of its closest relative, the
Silvery Blue (G. lygdamus). We compared these to a novel annotated
genome of the Green-Underside Blue (G. alexis). Phylogenetic
relationships inferred from complete mitochondrial genomes indicate that
Xerces Blue was a distinct species that diverged from the Silvery Blue
lineage at least 850,000 years ago. Using nuclear genomes, we show that
both species experienced population growth during the MIS 7 interglacial
period, but the Xerces Blue decreased to a very low effective population
size subsequently, a trend opposite to that observed in the Silvery
Blue. Runs of homozygosity in the Xerces Blue were significantly greater
than in the Silvery Blue, suggesting a higher incidence of inbreeding.
In addition, the Xerces Blue carried a higher proportion of derived,
putatively deleterious amino acid-changing alleles than the Silvery
Blue. These results demonstrate that the Xerces Blue experienced more
than 100 thousand years of population decline, prior to its
human-induced final extinction.
Changes in Sea Ice and Range Expansion of Sperm Whales in the Pond Inlet Region of Baffin Bay, Canada
Authors: Posdaljian Natalie , Soderstjerna Caroline , Jones Joshua , Solsona-Berga Alba , Hildebrand John , Westdal Kristin , Ootoowak Alex , Baumann-Pickering Simone
Published: January 2024

Sperm whales are a cosmopolitan species only found in ice-free regions.
Understanding the distribution of sperm whales in regions undergoing
rapid loss of sea ice and ocean warming is important. In 2014 and 2018,
sperm whales were sighted in the Pond Inlet, Baffin Bay: the first
recorded uses of this region. We investigated sperm whale distribution
near the Pond Inlet using visual sighting and passive acoustic data from
two recording sites between 2015 and 2019. We found no records of sperm
whale sightings near Pond Inlet outside of the 2014/2018 observations.
However, the acoustic data revealed whale presence yearly from 2015-2019
in the late summer and fall months. Sperm whale presence increased over
the 5-year study duration and was closely related to sea ice extent.
Monitoring climate change-induced range expansion of sperm whales is
important to understand how increasing presence of a top-predator might
impact the Arctic food web.
Structural variation detection of adolescent thyroid cancer using optical mapping
Authors: Yang Lei , Wu Shaochang , Liao Baosheng , Guo Shuai , Wang Shengcai , Liu Yuwei , Fan Jialin , Tai Jun , Chen Jun , Ni Xin , Xu Jiang
Published: January 2024

Abstract：Structural variation (SV) is a fundamental genetic cause for
cancer, with demonstrated correlation to disease progression and
treatment response. Traditional sequencing method cannot provide full
genomic landscape especially large-scale and complex structural
variation. To overcome these limitations, we adopted a combined
sequencing approaches, including optical mapping, single molecular
sequencing and short reads shotgun sequencing, to evaluate the SV in
thyroid cancer. Different numbers, length and types of structural
variation, with genes affected by SV were scrutinized. Integrating these
results could showed comprehensive scenario for thyroid cancer in a
genomic view. We demonstrate that integrated approaches could provide a
powerful tool for capturing a higher level of genomic SV, creating new
interpretation of sequencing data of particular relevance to human
cancer.
CONGENITAL HEPATOPULMONARY FUSION
Authors: Rocha Gustavo
Published: January 2024

Introduction – Congenital hepatopulmonary fusion (HPF) is a rare
anomaly characterized by a fusion between the liver and lung parenchyma.
HPF cases have been scarcely reported in the literature. Methods - An
extensive search of publications was performed in the PubMed and Google
Scholar databases without a time limit. Results – In total, 34 clinical
case reports were found in the literature, and a study by the CDH Study
Group reported data on 10 patients with congenital HPF. Of these 44
infants, 20 were male, 20 were female, and four were reported without
gender specification. Nineteen (43.2%) patients required intubation on
the first day of life. Six (13.6%) patients had their clinical
presentation during the first year of life, and four (9%) clinically
presented with HPF between 2.5 and 11 years old. In these patients,
cough, asthma-like symptoms, dyspnea, hemoptysis, right-side chest pain,
respiratory infections and pneumonia were the relevant clinical signs.
Right-lung vascular anomalies were present in 18 (40.9%) patients. A
complete liver and lung separation was successful in 17 (38.6%)
patients. The overall survival was 56.8%. Conclusion – Congenital HPF
has no gender predominance. In most cases, it behaves similar to a right
CDH in need of resuscitation and intubation after birth. The majority of
the cases are discovered during the surgical procedure for CDH. The best
surgical approach has not been established and depends on the degree of
fusion and vascular anomalies. An advanced imaging assessment is
necessary before a surgical approach is attempted. The prognosis is
ominous.
Adaptation and Validation of the ABC taxonomy for medication adherence into Portuguese
Authors: Bernardo Catarina , Tosin Michelle , Almada Marta , Sampaio Rute , Oliveira Beatriz , Costa Elisio , Vrijens Bernard , Costa Filipa Alves
Published: January 2024

Aim: To cross-culturally validate the ABC taxonomy into Portuguese for
Portugal and Brazil without questioning its original meaning. Methods: A
systematic review was conducted to identify published taxonomy terms and
definitions in Portuguese, and to identify experts in medication
adherence. Initial mapping of terms and definitions retrieved was
scrutinized by the research team to build an e-survey. The e-survey was
first piloted and then sent to experts in both countries seeking
consensus using a three-round Delphi technique. Consensus was defined as
≥ 85% for the first round and ≥75% for the second. Terms with
agreement lower than 10% were dropped between rounds. In the final
round, terms and definitions reaching agreement between 50-75% were
classified as moderate agreement and above 75% as strong agreement.
Results: A total of 778 studies were identified and 84 included,
enabling the extraction of 154 terms and 32 definitions. In the
first-round 164 experts participated, 115 in the second-round and 99 in
the third round. Strong consensus was obtained in Portugal for six terms
and five definitions and moderate consensus for one term and two
definitions. In Brazil, strong consensus was obtained for five terms and
definitions and moderate consensus for two terms and definitions.
Conclusion: A unified ABC taxonomy in Portuguese was possible to develop
and validate for use in Portugal and in Brazil. Its use will harmonise
and standardise the terms and definitions used in clinical practice and
research.
Problem of determining two relaxation functions in the integro - differential equation of rigid heat conductor
Authors: Zhumaev Zhonibek , Durdiev Durdimurod
Published: January 2024

The inverse problem of determining the energy-temperature relation a(t)
and the heat conduction relation k(t) functions in the one-dimensional
integro–differential heat equation are investigated. The direct problem
is the initial-boundary problem for this equation. The integral terms
have the time convolution form of unknown kernels and direct problem
solution. As additional information for solving inverse problem, the
solution of the direct problem for $x = x_0$; $x = x_1$ are given.
At the beginning an auxiliary problem, which is equivalent to the
original problem is introduced. Then the auxiliary problem is reduced to
an equivalent closed system of Volterra-type integral equations with
respect to unknown functions. Applying the method of contraction
mappings to this system in the continuous class of functions, we prove
the main result of the article, which is a local existence and
uniqueness theorem of inverse problem solutions.
A prey–predator–parasite model with fear effect and harvesting (hunting)
Authors: gao jianzhong , Liu Yanping , Zhang Tailei , Wang Ruiwu
Published: January 2024

In this paper, we analyze a predator-predator-parasite model, which
considers prey subjected to predator fear effect and two modes of
harvesting (cooperation hunting by predators and artificial harvesting
of prey). Firstly, mathematical analysis of the model with regard to the
non-negativity, boundedness of solutions, stability of equilibria,
permanence, backward bifurcation and Hopf-bifurcation of the model are
analyzed. Secondly, we obtain some numerical simulation results: we
conduct extensive numerical simulations to explore the roles of fear
effect and other biologically related parameters (e.g. disease
transmission rate of prey, hunting cooperation and artificial
harvesting), we find that low levels fear and hunting cooperation can
stabilize the eco-epidemiological system, and low level artificial
harvesting will produce chaotic oscillations, but high level artificial
harvesting will stabilize the eco-epidemiological system. In reality, we
should protect vulnerable species from fear and cooperation hunting by
predators, because decrease in the number of vulnerable species may lead
to decrease in species diversity. Moreover, artificial harvesting of
species with certain economic value is generally carried out (such as
fish populations). However, if the species density is too low, the
chaotic oscillation of the population is dangerous to the survival of
the population, increasing the probability of population extinction, and
it is impossible to obtain a constant yield from the fluctuating
population. Therefore, it is desirable to promote the overall stable
equilibrium state of the system by artificial harvesting. In addition,
it is also observed the presence of disease in an ecosystem can promote
stable coexistence of species.
Reaction network and molecular distribution of sulfides in gasoline and diesel of FCC process.
Authors: Qin Xinglong , Ye Lei , Murad Alqubati , Liu Jichang , Ying Qiang , Long Jian , Yu Wenxin , Xie Jinquan , Hou Lixin , Pu Xin , Han Xin , ZHAO Jigang , Sun Hui , Ling Hao
Published: January 2024

In order to guide the accurate control of the sulfides in gasoline and
diesel of Fluid Catalytic Cracking (FCC) process, a molecular-level
model was established based on the Structure Oriented Lumping (SOL)
method. According to the molecular composition characteristics of FCC
feed oil, a molecular composition matrix containing 4,148 row vectors
was constructed with 24 structural increments. Using MATLAB software,
the SOL reaction rules were compiled and a complex reaction network
containing of about 110,000 reactions was established. According to the
classification rules, the sulfides in gasoline and diesel represented by
32 and 136 structural vectors were divided into mercaptans, thioethers,
monocyclic thiophenes, benzothiophenes and dibenzothiophenes,
respectively. The conversion law of sulfides in gasoline and diesel was
investigated by tracking their generation paths and reaction paths in
the reaction network. The effects of the operation conditions on the
sulfide contents in gasoline and diesel were calculated quantitatively.
Caplacizumab Treatment in a Case of Resistant Thrombotic Thrombocytopenic Purpura
Authors: Hossein-zadeh Zarrin , Rafiee Behnam , Ram Bebu , Weltz Jonathan , Friedman Mark
Published: January 2024

A 27-year-old G2P1 woman, admitted at 37 weeks of gestation after
complaints of nausea, vomiting, and vaginal bleeding. An emergent
vaginal delivery was performed which was complicated by significant
blood loss. Thrombotic thrombocytopenic purpura was thought to be the
most probable cause, requiring administration of caplacizumab and daily
TPE.
Long-term development of children born to women with twin pregnancies treated with cervical pessary or vaginal progesterone: follow-up of a randomized controlled trial
Authors: Tran Van , Nguyen Nghia , Nguyen Nam , Vo Thu , Uong Truong , Nguyen Hau , Nguyen Ngan , Pham Toan , Nguyen Minh , Nguyen Linh , Vuong Lan , Mol Ben , Dang Vinh
Published: January 2024

Objective: To compare the effectiveness of cervical pessary and vaginal
progesterone in improving long-term developmental outcomes of children
born to women with twin pregnancies and mid-trimester short cervical
length (CL). Design: Randomized controlled trial (RCT). Setting: My Duc
hospital, Ho Chi Minh City, VietNam. Population: Children born to women
in an RCT comparing cervical pessary and progesterone (NCT02623881).
Methods: At or after 2 years of age, their parents completed a validated
Vietnamese version of Ages & Stages Third Edition Questionnaires
(ASQ-3) and Red flag questionnaire. Main Outcome Measures: Mean ASQ-3
scores, red flag signs, and the composite outcome of perinatal death or
survival with any abnormal ASQ-3 score. Results: In original RCT, we
randomized 300 women to pessary (n=150) or progesterone (n=150). After
counting the number of perinatal death and lost to follow-up, in pessary
group, 116 (82.8%) parents of 229 children returned the questionnaire
versus 118 (82.5%) parents of 232 children in progesterone group. Mean
ASQ-3 scores of the five skills, as well as red flag signs, did not
differ significantly between the two groups. However, the percentage of
children having died or having abnormal ASQ-3 scores in fine motor was
significantly lower in the progesterone group (1.7% vs 6.1%, P=0.01).
In an exploratory analysis, there were no significant differences in the
composite outcome of perinatal death or survival with any abnormal
ASQ-3. Conclusions: Cervical pessary and progesterone have a comparable
effect on developmental outcomes in ≥24 months of children born to women
with twin pregnancies and short CL.
Clinical presentation, hematologic characteristics and survival outcome of childhood leukemia with musculoskeletal involvement: A 42-year experience from a single tertiary center in Thailand
Authors: Chotsampancharoen Thirachit , Kittivisuit Sirinthip , Sripornsawan Pornpun , Songthawee Natsaruth , Chavananon Shevachut , McNeil Edward
Published: January 2024

Abstract Background: Childhood leukemia with musculoskeletal (MSK)
involvement mimics various conditions, which consequently leads to
diagnostic delays. The clinical implication of MSK involvement in this
disease on survival outcomes is inconclusive. This study aimed to
compare characteristics and survival outcomes between MSK and non-MSK
involvement in childhood leukemia. Methods: The medical records of
children newly diagnosed with acute leukemia aged under 15 years were
retrospectively reviewed. Two-to-one nearest-neighbor propensity
score-matching was performed to obtain matched groups with and without
MSK involvement. The Kaplan-Meier method and log-rank test were then
used to assess the effect of MSK involvement on survival outcomes.
Results: Of 1042 childhood leukemia cases, 81 (7.8%) children had MSK
involvement at initial presentation. MSK involvement was more likely in
children with acute lymphoblastic leukemia than acute myeloid leukemia
(p <0.05). Hematologic abnormalities were less frequent in the
MSK involvement group (p <0.05). The absence of peripheral
blast cells was significantly higher in the MSK involvement group
(17.3% vs 9.6%, p = 0.04). Normal complete blood counts with absence
of peripheral blast cells were found 2.5% of the children with MSK
involvement. By propensity score-matching for comparable risk groups of
children with and without MSK involvement, the 5-year overall survival
was not significantly different (48.2% vs 57.4%, respectively, p =
0.22), nor was event-free survival (43.3% vs 51.8%, respectively, p =
0.31). Conclusion: Childhood leukemia with MSK involvement had the
characteristics of minimal or absent hematologic abnormalities and
peripheral blast counts.
Sweet Syndrome following SARS-CoV-2 CoronaVac vaccine
Authors: Nesrine Ben Salah , Korbi Mouna , Fadhel Najeh Ben , Safa Idoudi , Chahed Ferdaous , Njima Manel , Belhadjali Hichem , Amri Montassar , Aouam Karim , Zili Jameleddine
Published: January 2024

We reported the 5 published case of COVID-19
vaccine inducing SS and the first case to be induced by an inactivated
SARS-CoV-2 vaccine « CoronaVac ». The short latency time strongly
supports the pathogenic role of the vaccine as a cause of SS in our
case.
Co-infection of Mammalian orthorubulavirus 5 and Mammalian orthoreovirus type-3 in domestic pigs in India
Authors: Singh Fateh , RAJUKUMAR KATHERUKAMEM , D Senthilkumar , G Venkatesh , Shrivastava Deepali , Kombiah Subbiah , Jhade Sandeep Kumar , Singh Vijendra
Published: January 2024

During a routine surveillance for exotic and emerging swine viral
diseases in India, five samples (four faecal, one serum) showed
cytopathic effects in Vero cells. Transmission electron microscopy of
infective cell supernatant revealed the presence of two types of
virions. De novo metavirome sequencing enabled complete genome
assembly of Mammalian orthorubulavirus 5 (MRuV5) and Mammalian
orthoreovirus (MRV). The MRuV5 isolates possessed a whole genome of
15246 bp with seven genes (NP, V/P, M, F, SH, HN, L), while the MRV
isolates had segmented genome with three large (L1, L2, L3), three
medium (M1, M2, M3) and four small (S1, S2, S3, S4) segments. The MRuV5
isolates were genetically grouped with those from various mammalian
species reported from South Korea and China. Deduced amino acid
sequences of the HN, NP and F gene coding regions of MRuV5 isolates
showed the substitutions of three (92L, 111R, 447H), two (86S, 121S) and
two (139T, 246T) amino acids, respectively, compared to previously
reported strains of the virus. However, they did not reveal any change
in predicted amino acid residues at the receptor binding site in HN
protein, cleavage site or HN stalk region. S1 gene of the MRV isolates
showed the highest nucleotide identity (97.73%) with the MRV3 strain
ZJ2013 isolated from pig in China, and these isolates were grouped into
MRV type-3. Deduced amino acid sequences of MRV3 S1 gene revealed amino
acid residues 198-204NLAIRLP, 249I, 340D, 419E known for sialic acid
binding site and neurotropism. We report the co-infection of MRuV5 and
MRV3 detected incidentally for the first time in domestic pigs in India.
Although MRuV5 and MRV3 develop asymptomatic infections or mild disease
in animals and humans, continuous monitoring of evolution and spread of
such viruses is important in the current global scenario of increasing
threats due to emerging novel pathogens.
MERGERS & ACQUISITIONS IN ELEVATOR INDUSTRY: THE ROLE OF ICTs TO AVOID INFORMATION ASYMMETRY
Authors: Guardia Juan de la , Palacios Miguel , Zurdo Ricardo
Published: January 2024

Mergers and Acquisitions operations continues to be one of the most
explored growth strategies globally in all markets, and also in the
Elevator Industry. Volume of investments grows year after year. It is
the fastest way to grow in international and domestic markets, but the
reality is that a high percentage of the operations carried out do not
meet the expectations of the investors when the integration phase,
called the post-acquisition phase, has been completed. There are several
causes of failures on mergers and acquisitions processes, such as lack
of commitment from the management, unrealistic business plan, cultural
shock, etc … But the most common and the one with the highest
risk in an acquisition decision, is the asymmetry of information. During
the negotiation phase a large amount of data is collected, and
subsequently analyzed during the Due Diligence period, but does not
correspond to reality during the integration phase. That is, the
information that the seller has provided, and has been studied and
analyzed to take a decision of acquire by investors, has not been the
real and complete one. In this article we will propose how the asymmetry
information can be avoided by the application of Information and
Communication Technology (ICT) through the IoT of the elevators. Same
can be applied to other industries.
siRNA transfection and shRNA transduction optimization for CXCL12 mRNA silencing in human bone marrow mesenchymal stem cells
Authors: Nascimento Sabrina do , Mansur Fernanda , Rocha Fernanda , Marti Luciana
Published: January 2024

RNA interference (RNAi) is a gene-silencing process elicited by
double-stranded RNAs. This methodology can be used for analyzing
loss-of-function phenotypes and has highly contributed for cells
molecular biology research field. This study was conducted in order to
optimize siRNA transfection and virus-mediated short-hairpin RNA
(vshRNA) for silencing CXCL12 mRNA in human bone marrow mesenchymal stem
cells (BM-hMSC) intending further studies on its role in hematopoietic
stem cells (HSC) in the bone marrow niche. CXCL12 mRNA levels transient
reduction was observed after siRNA transfection for silencing CXCL12
mRNA. However, there were no significant differences in intracellular
CXCL12 protein levels or in cultures supernatant. On the other hand, the
transduction of CXCL12-targeting vshRNA resulted in downregulation of
the CXCL12 mRNA and protein levels in both intracellular compartment and
culture supernatant of BM-hMSCs. Our results demonstrated very high
level of transduction efficiency, without repeated rounds of infection,
neither cell sorting nor antibiotic selection and with no major changes
in cell viability or morphology. Thus, the current study presents an
effective method for functional virus transduced BM-hMSC. In addition,
this study discusses the requirement for testing candidate sequences and
shRNA screening before confirming their effectiveness. Additionally,
this study demonstrated that a gene reporter as green fluorescent
protein (GFP) signal is not necessary closely correlated with knockdown
efficiency.
Patient Satisfaction of Ambulatory Care Pharmacy Services in Tabuk City, Saudi Arabia
Authors: Ali Mostafa , Amirthalingam Palanisamy , Alatawi Yasser , Aljabri Ahmed
Published: January 2024

Rationale, Aims, and Objectives: To explore the patient satisfaction of
outpatient care services at Tabuk, Saudi Arabia. Methods: A
cross-sectional survey was conducted at two tertiary care hospitals
located in Tabuk, Saudi Arabia over three months duration. A
semi-structured questionnaire composed of eight different domains of
pharmaceutical care services through face-to-face patient interview and
electronic format using website. Results: A total of 508 participants
responded to the survey. Among the respondents, male gender (51.8%) was
predominant and most of them (83%) were below 46 years old. The average
scores of domains 1, 2, 3, 4, 5, 6, 7, and 8 were 4.51, 4.11, 4.30,
3.70, 3.57, 3.82, 3.63, and 4.15, respectively. The general evaluation
of pharmacy services domain was found to be very satisfied (245; 48.2%)
and satisfied (143; 28%) with 380 (76.4%) respondents were willing to
recommend the pharmacy to their family or friends. There was no
statistically significant difference in regard to the overall
satisfaction of the respondents in all domains of pharmacy services
among the two hospitals (p>0.05). Discussion: The majority
of respondents were satisfied with outpatient care pharmacy services at
Tabuk, Saudi Arabia. More efforts are needed to improve the service of
following-up on patients’ adherence by the pharmacists.
Improved survival of pediatric Hodgkin lymphoma in developing countries using Combined-Modality Therapy with ABVD/COPDac: a single institution study in Iraq
Authors: Al-Jumaily Usama , Shukur Haider , Fawzi Mohammed , Jawad Mohammed , Al-Safi Rasha , Al-mosawy Sabah , Al-Hashemy Tammam , Faraj Safa
Published: January 2024

Purpose Data about Hodgkin lymphoma (HL) in developing countries are
scattered. The aim of this study is to present the clinical features and
outcome of children with HL using combined different chemotherapy
regimens. Response-based approach was applied irrespective of risk
stratification. Methods Patients aged 18 years and younger who were
diagnosed with HL between January 2014 and December 2019 in the main
tertiary hospital in Kerbalaa, Iraq, were recruited. Patients were
stratified into three risk groups. Initial treatment with induction
chemotherapy (i.e., 2 cycles of ABVD) was applied to all patients.
Patients who achieved complete radiological response to induction
chemotherapy continued with 4-6 cycles of ABVD; radiotherapy was
omitted. Patients with slow early response received 3 cycles of COPDac
after 3rd cycle of ABVD, followed by radiotherapy. Results Forty-one
patients were enrolled in this study. The median age was 7 years.
Fourteen (34.1%) patients were stage III, followed by stage I (29.3%).
Eighteen patients had B symptoms. Initial splenic involvement was found
in 11 patients. Approximately one third of patients had bulky disease (n
= 12; 29.3%). The most common histology was mixed cellularity (n = 29).
The median follow-up was 1.25 year (range from 0.5 to 2.0 years). The
4-year estimates of survival and EFS were 92% and 84% ±10%,
respectively. Only bulky disease had a significant negative influence on
the outcome. Conclusions Good outcomes for pediatric HL patients can be
achieved in low and middle income countries. Response-based therapy
approach is feasible to reduce long term treatment-related sequel.
Study on generalized variable coefficient fifth-order KdV equation based on higher order dispersion term
Authors: Zhao Zhen , Pang Jing
Published: January 2024

Nonlinear partial differential equations with higher order dispersion
terms play an important role in dynamics research. In this paper, the
fifth order KdV equation with high order dispersion term is studied and
discussed. Firstly, the bilinear form of the fifth order KdV equation
with high order dispersion term is derived by Hirota bilinear form.
Then, the combined test function of the positive quartic function,
quadratic function, exponential function and the interaction solution of
the hyperbolic function of the fifth order KdV equation with variable
coefficients is constructed, and the resonance multi-soliton test
function of the equation is constructed by using the linear
superposition principle.By means of mathematical symbol calculation, the
interaction solution between high-order Lump solution and periodic cross
kink solution of the fifth order KdV equation with variable coefficients
and its resonance multi-solitons are solved.And by observing its
corresponding graph analysis of its physical phenomenon.
Asynchronous tele-expertise (ASTE) for prenatal diagnosis is feasible and cost saving: results of a French case study
Authors: BELDJERD M'hamed , LAFOUGE Antoine , GIORGI Roch , SORIANO Anne Gaelle LE CORROLLER , Quarello Edwin
Published: January 2024

Objective: The objective of this study was to assess the potential of
the use of asynchronous tele-expertise (ASTE) to provide prenatal
diagnosis from a medical and economic point of view. Population:
Patients screened by a midwife at a primary center. Methods: A technical
and clinical evaluation was conducted retrospectively, and a cost
minimization study compared asynchronous tele-expertise to face-to-face
consultations that would have been performed without ASTE. Main outcome
measures: In our study we assessed the feasibility of TEAS, what were
the origins of the requests for expertise, whether patients need to be
moved and the reasons for doing so, and the costs of tele-expertise and
conventional consultation. Results: In this retrospective analysis 322
advices from 260 patients were interpreted remotely via a platform. The
results revealed a 90.68% feasibility of transmitting in a satisfactory
and interpretable way ultrasound images and videos via the
tele-expertise platform (292/322 files). In our series, asynchronous
analysis allowed the required physician to make an accurate diagnosis
and identify 74 (28.5%, 95% CI [23% –33.9%]) pregnancies
associated with malformations and rule out abnormalities in 186 (71.5%,
95% CI [66.1% –77%]) of the cases. Asynchronous analysis
prevented the displacement of 72.7% (189/260) patients. The practice of
ASTE would result from a societal point of view, an average saving of
\euro 123.40 per patient. Conclusion: The use of
asynchronous tele-expertise (ASTE) using fetal ultrasound, is feasible
and may contribute to increased diagnostic accuracy while generating a
significant reduction in costs for society. Funding: None
Higher order approximation of derivatives based on numerical integration
Authors: Xu Qiuyan , Liu Zhiyong
Published: January 2024

Different from the construction process of the traditional finite
difference method, we derive a large class of high-accuracy methods for
approximating derivatives. Since Taylor expansion is avoided, the
requirement for function smoothness in the new methods is greatly
reduced. We analyze the approximation errors of the proposed methods and
compare their approximation effects. As a typical application, we use
the proposed methods to solve elliptic, hyperbolic and parabolic
problems numerically. For time-dependent problems, a class of new fully
implicit difference schemes and the fourth-order Runge-Kutta method are
both used for discretization. When using fully implicit difference
schemes, the unconditional stability and convergence are proved for
hyperbolic and parabolic problems with the periodic boundary conditions.
A large number of numerical experiments are provided to demonstrate the
theoretical results.
Antiemetic activity of Fosaprepitant as a rescue agent in patients with postoperative nausea and vomiting after orthopedic surgery
Authors: Saleh Jawad
Published: January 2024

Backgroud: Postoperative nausea and vomiting (PONV) is a common
postoperative complication in the surgical setting. Many agents exist
for PONV prophylaxis but rescue options remains a big challenge.
Fosaprepitant has been studied in PONV prophylaxis but the data is
limited when utilized for rescue. Methods: In this single center
retrospecitve chart review, the antiemetic activity of fosaprepitant
(selective NK1 receptor antagonist) was investigated as a rescue agent
for PONV in patients undergoing orthopedic surgery. Based on the
institutional protocol, fosaprepitant was only utilized in patients with
an existing history of PONV or when 2 antiemtic agents were exhausted
with no success in the postoperative period. The groups evaluated are
inferred to be patients with and without emesis relief. Chart reviews,
progress notes and clinical interventions were utilized to assess emesis
relief. Results: 383 total patients (over a 12 month period) fell into
the hospital protocol and approved to receive fosaprepitant as a rescue
agent. Out of the 383 patients, 286 had relief with fosaprepitant, and
196 patients did not require further antiemetic therapy. Patient
demographs, anesthesia type, additional medications and surgical types
were all assessed thoroughly. Three surgical types showed the most
prominent significance for fosaprepirtant PONV relief: Sports management
and shoulder 76% (p = 0.0012). Adult Reconstruction and Joint
Replacement 74% (<0.0001), Spine 61% (p = 0.015),
Conclusion: The data shows their may be potential for utilizing
fosaprepitant as a rescue agent for PONV in the orthopedic setting. A
bigger study with a larger sample size is warranted.
The ‘Umbrella Furling’ Deflation Technique for Cuffed Tracheostomy Tube - A Useful Tip for An Easier Tracheostomy Insertion
Authors: Hong Estelle How , Li Lucy , Hilmi Omar , Haddow Kerry
Published: January 2024

Key points: • When deflated, folds often form in the balloon cuff of a
tracheostomy tube resulting in additional friction when inserting the
tube. • Furthermore, this potentially increases the risk of cuff
rupture, particularly in patients with calcified tracheas and iatrogenic
tracheal injury from using excessive physical force when inserting a
tracheostomy tube. • The ‘Umbrella Furling’ technique for deflating the
cuff of a tracheostomy tube is a simple approach whereby the cuff is
slowly deflated with a damp gauze wrapped around it while simultaneously
twisting the tracheostomy tube. • This enables the deflated cuff to sit
flush to the tube, therefore allowing an easier “reduced-friction”
insertion of a cuffed tracheostomy tube and reducing the risk of damage
to the cuff. • The same technique can be used to facilitate the
insertion of endotracheal tubes through an enclosed laryngoscope.
Auricular Acupressure for Pain Relief in Labour : A Systematic Review and Meta-Analysis
Authors: WANG ZHE , Zheng Hao , Li Yanan , Lv Hao , Wang Xiaoyan , Han Jing
Published: January 2024

ABSTRACT Background Labour pain ranks consistently among the most severe
types of pain that a woman will experience during her lifetime and leads
harmful effects on both mother and baby. As a complementary method,
auricular acupressure has been used in the pain management for many
diseases and many RCTs showed it has a pain-reliving effect in labour.
However, no meta-analysis has been conducted to provide systematic
evidence for pain relief in labour. Objectives To perform a systematic
review to assess the efficacy of auricular acupressure for labor pain.
Search Strategy PubMed, Cochrane Library, Embase, Web of Science
databases, SinoMed, CNKI, WanFang Data and VIP were searched for studies
using keywords “auricular acupressure” combined with “labor” and
“RCTs.” Selection Criteria: Eligible criteria included RCTs, full-text
studies, English, and Chinese literature, whereas exclusion criteria
included incomplete information, duplicated publications, and studies
combined with other analgesic methods. Data Extraction and Analysis: The
selection of eligible items and assessment of methodological quality
were performed independently by two researchers. A meta-analysis was
performed to analyze the treatment effects on pain intensity reduction,
maternal satisfaction and safety. Main Results: 17 RCTs were included in
this review involving a total of 2574 parturients. Auricular acupressure
showed significant efficacy in the reduction of pain intensity and
improvement of maternal satisfaction. Conclusions: Auricular acupressure
showed a pain-relieving effect compared with the routine care. Although
the evidence is limited and high quality studies are needed.
Design and Implementation of the Sickle Cell Disease Hematopoietic Cell Transplantation Evaluating Long Term and Late Outcomes Registry (STELLAR) to Compare Long Term Outcomes after Hematopoietic Cell Transplantation to that in Siblings without Sickle Cell Disease and in Non-Transplanted Individuals with Sickle Cell Disease
Authors: Krishnamurti Lakshmanan , Arnold Staci , Haight Ann , Abraham Allistair , Guilcher Gregory , John Tami , Bakshi Nitya , Shenoy Shalini , Syrjala Karen , Martin Paul , Chaudhury Sonali , Eames Gretchen , Olusola Olowoselu , Fuente Josu De La , Hsieh Matthew , Kasow Kimberly , Mertens Ann , El-Rassi Fuad , Lane Peter , Shaw Bronwn , Meacham Lillian , Archer David
Published: January 2024

Background There are sparse data on long-term and late effects of
hematopoietic cell transplantation (HCT) for sickle cell disease (SCD)
Objectives To establish an international registry of long-term outcomes
post-HCT for SCD and demonstrate the feasibility of recruitment at a
single site in the US. Methods The STELLAR registry is designed to
enroll SCD patients ≥ 1-year post-HCT, their siblings without SCD, and
non-transplanted SCD controls to collect participant self-report of
health status and practices using the BMT survivor study surveys, HRQOL
using PROMIS 25 or 29, cGVHD using the symptom scale survey, daily pain
using an electronic pain diary, economic impact of HCT using the
financial hardship survey, and sexual function using PROMIS SexFSv2.0.
We also piloted retrieval of clinical data previously submitted to
CIBMTR, recorded demographics, height, weight, BP, hip and waist
circumference, timed-up-and-go, and handgrip test, and obtained blood
for metabolic screening, gonadal function, fertility potential, and
biorepository of plasma, serum, RNA, and DNA. Results Among 100 eligible
post-HCT patients, we enrolled 72 participants 9-38 (median 17) years
age. We also enrolled 19 siblings 5-32 (median10)years age and 28
non-transplanted SCD controls 4-46 (median 22) years age. Of 119
participants, 73 completed 85 sets of surveys and 41 contributed samples
to the biorepository. We successfully piloted retrieval of data
submitted to CIBMTR and expanded recruitment to seven US, Canada, UK,
and Nigeria sites. Conclusions It is feasible to recruit subjects and
conduct study procedures for the STELLAR registry of long-term and late
effects of HCT for SCD.