bluuebunny/crossref_metadata_2025 huggingface dataset:
Implementing Smart Statistics Toolkit in the Official Statistics
Authors: Osaulenko О. H. , Horobets О. О.
Published: September 2023

Important issues of Smart statistics are addressed. The Smart statistics toolkit is analyzed: big data, data of artificial intellect and Internet of things, social media, and administrative data. 

The study involves conceptualization of the Smart statistics and identification of advantages and threats for the official statistics in using Smart statistics data. A set of principles for operating big data are proposed, with categorizing the sources of big data generation (in conformity with the economic activities), presently demanded by the official statistics and the society (agriculture, health protection, mining industry, mechanical engineering, education, power industry). A smart data system in smart cities is developed, containing the following components: smart house, smart environment, smart control, smart traffic, smart health, and smart citizen. It is determined that the system’s objective is to create the smart environment and simplify the way of life through saving time, energy and money. The components of personalized collection and dissemination of data are determined.   

The artificial intelligence (AI) is considered as a component of the Smart statistics conception. In this article’s context, the authors observe that using AI causes ethical discourses on issues such as property right for data, transparence and accountability of data. These discourses need to accounted for, in order to assure that AI technologies are used by the official statistics in a responsible and ethical manner. 

Based on the results of the study, it is concluded that now it is necessary to create logical and adequate approaches to elaborating a methodology for collection, processing, grouping and analysis of statistical data from alternative information sources. It is argued that in spite of the advantages of the smart statistics toolkit and potentially positive results of its implementation in the official statistics, present-day Ukraine is not ready to face the threats involved in it given the war conditions and lack of a regulatory framework for its use.

Further studies on this theme have to focus on in-depth analysis of Smart statistics and search for optimal ways for implementing its toolkit in the official statistics of Ukraine.
Наука та аудит. Гештальт ще не закрито
Authors: Redko О. Yu.
Published: September 2023

The article critically analyzes the state of modern Ukrainian audit science. It is noted that over the past five years there have been 310 defenses of theses in the specialty "Accounting, analysis and audit", of which only 8% were devoted exclusively to audit topics, including only 3% for obtaining the scientific degree of Doctor of Economic Sciences. Analyzing the declared topic of scientific research, the author draws attention to the fact that scientists research questions that are, so to speak, "by ear" in the professional environment. This is mainly a question of audit quality and its external and internal control. Unfortunately, there are no studies that would develop, first of all, the audit methodology, its methods and procedures, the peculiarities of auditing in computer networks. The author notes the parallel existence of audit science and practice. The reason given is the impossibility of combining scientific research with practical audit activity, the lack of interest of audit practitioners in the results of scientific research. Analyzing the scientific work of institutions of higher education, the author states the parallel existence of audit science and practice. The reason given is the impossibility of combining scientific research with practical audit activity, the lack of interest of audit practitioners in the results of scientific research. Analyzing the scientific work of institutions of higher education, the author singles out a small number of centers where audit is researched, among which examines the work of the Audit Scientific School of the National Center for Accounting and Audit of the National Academy of Statistics, Accounting and Audit. In the article, the author makes several proposals that, in his opinion, can improve the interaction of Ukrainian science and auditing practice.
PENGARUH MODEL PEMBELAJARAN SINEKTIK TERHADAP KEMAMPUAN MENULIS PUISI SISWA KELAS VIII SMP PATRA MANDIRI 2 PALEMBANG
Authors: Amelia Kiki
Published: September 2023

PENGARUH MODEL PEMBELAJARAN SINEKTIK TERHADAP KEMAMPUAN MENULIS PUISI SISWA KELAS VIII SMP PATRA MANDIRI 2 PALEMBANGOleh: Kiki Amelia1Hetilaniar 2 MasnunahMTs An Nur Palembang, Universitas PGRI PalembangÂ Kikiamelia01101997@gmail.com, Heti_ardesya@yahoo.com, Masnunah42@gmail.comÂ AbstrakTujuan penelitian ini untuk mengetahui adanya pengaruh model pembelajaran sinektik terhadap kemampuan menulis puisi siswa kelas VIII SMP Patra Mandiri 2 Palembang. Metode penelitian yang digunakan yaitu metode True EksperimentalÂ  Design Tipe Posstest Only Control Design. Teknik analisis data yang digunakan adalah teknik tes dan dokumentasi. Hasil Penelitian terhadap kemampuan menulis puisi siswa kelas VIII SMP Patra mandiri 2 Palembang. Hasil nilai perhitungan data belajar siswa menggunakan uji-t (T Test dari Anova) yang terdapat pada sebuah program SPSS versi 19. Menunjukkan bahwa yang berarti jika nilai Sig > 0,05 maka distribusi data homogen, dan jika nilai Sig < 0,05 maka distribusi data tidak homogen. Hasil perhitungan nilai signifikan diatas nilai yang diperoleh ternyata uji-t 0,54 > 0,05. Berarti nilai Sig > 0,05 maka peneliti menyimpulkan bahwa â€œAda Pengaruh Model Pembelajaran Sinektik Terhadap Kemampuan Menulis Puisi Siswa Kelas VIII SMP Patra mandiri 2 Palembangâ€.Kata Kunci : Model pembelajaran sinektik,Kemampuan menulis,PuisiTHE INFLUENCE OF SYNECTIC LEARNING MODEL TO WRITING ABILITY OF POETRY STUDENTS IN CLASS VIII SMP PATRA MANDIRI 2 PALEMBANGAbstractÂ Â Â Â Â Â Â Â Â Â Â Â Â Â  The purpose of this study was to determine the effect of synectic learning models on the ability to write poetry for eighth grade students of Patra Mandiri 2 Junior High School in Palembang. The research method used is the True Experimental Design Type Posstest Only Control Design. The data analysis technique used is the test and documentation technique. Research Results on the ability to write poetry for eighth grade students of Patra Mandiri 2 Junior High School in Palembang. The results of the calculation of student learning data using the t-test (T Test from Anova) contained in an SPSS version 19. It shows that which means that if the Sig value> 0.05 then the homogeneous data distribution, and if the Sig value <0.05 then data distribution is not homogeneous. The result of calculating the significant value above the value obtained turns out to be t-test 0.54> 0.05. Meaning a Sig value> 0.05, the researcher concludes that "There is an Effect of a Synectic Learning Model on the Ability to Write Poetry in Class VIII Middle School Independent Patra 2 Palembang Students".Keywords: Synectic learning model,Writing ability,PoetryÂ
ANALISIS NILAI SOSIAL CERITA ANDAI-ANDAI DI KECAMATAN TANJUNG SAKTI PUMI KABUPATEN LAHAT SUMATERA SELATAN
Authors: Pebrianti Ike Tri , Triana Selpi
Published: September 2023

ANALISIS NILAI SOSIAL CERITA ANDAI-ANDAI DI KECAMATAN TANJUNG SAKTI PUMI KABUPATEN LAHAT SUMATERA SELATANÂ Ike Tri Pebrianti(1), Selpi Triana(2)STKIP Muhammadiyah Pagaralamikek22@yahoo.comselvitriana888@gmail.comABSTRAKCerita rakyat merupakan prosa yang disampaikan secara lisan. Cerita rakyat lebih dikenal masyarakat sebagai dongeng, legenda atau cerita lisan yang berlatar belakang sejarah. Cerita rakyat ini, hidup dan berkembang dalam masyarakat tanpa mengetahui siapa pengarangnya. Masalah dalam penelitian ini adalah bagaimanakah nilai sosial dalam cerita andai-andai di Kecamatan Tanjung Sakti PUMI Kabupaten Lahat Sumatera Selatan. Tujuan penelitian untuk mendeskripsikan nilai sosial dalam cerita andai-andai di Kecamatan Tanjung Sakti PUMI Kabupaten Lahat Sumatera Selatan. Metode yang digunakan adalah metode deskriptif kualitatif. Teknik pengumpulan data menggunakan teknik catat, simak, rekaman, dan studi kepustakaan. Sumber data yang digunakan berupa cerita andai-andai di Kecamatan Tanjung Sakti PUMI Kabupaten Lahat Sumatera Selatan yang diceritakan secara lisan dalam bahasa daerah (Besemah) oleh penduduk asli Kecamatan Tanjung Sakti PUMI Kabupaten Lahat Sumatera Selatan. Hasil penelitian menunjukkan dalam andai-andai â€œBawang Puteh nga Bawang Abangâ€ nilai sosialnya adalah kepatuhan anak pada tiri ibunya. Selanjutnya pada andai-andai â€œBatu Belah Batu betangkupâ€ adalah sikap pelit yang tidak boleh ditiru.Kata KunciÂ  : Analisis, Nilai, Cerita ANALYSIS OF THE SOCIAL VALUE OF THE IFLS STORY IN KECAMATAN TANJUNG SAKTI PUMI, LAHAT DISTRICT, SOUTH SUMATERAÂ ABSTRACTFolklore is a prose that is conveyed orally. Folk stories are better known to the public as fairy tales, legends or oral stories with historical backgrounds. This folklore lives and develops in society without knowing who the author is. The problem in this research is what is the social value in the supposing story in Tanjung Sakti PUMI District, Lahat Regency, South Sumatra. The purpose of this research is to describe the social value in the ifsay story in Tanjung Sakti District, PUMI, Lahat Regency, South Sumatra. The method used is a qualitative descriptive method. The data collection technique uses the technique of note-taking, listening, recording, and literature study. The data source used was a supposing story in Tanjung Sakti PUMI Subdistrict, Lahat Regency, South Sumatra which was told orally in the regional language (Besemah) by the natives of Tanjung Sakti PUMI Subdistrict, Lahat Regency, South Sumatra. The results showed that the social value of "Bawang Puteh nga Bawang Abang" is the child's obedience to the mother's steps. Furthermore, supposing "Batu Belah Batu betangkup" is a stingy attitude that should not be imitated.Keywords: Analysis, Value, StoryÂ
PEMERTAHANAN BAHASA MUSI DI DESA SUKA DAMAI KECAMATAN PLAKAT TINGGI KABUPATEN MUSI BANYUASIN
Authors: Pramita Iswinda
Published: September 2023

PEMERTAHANAN BAHASA MUSI DI DESA SUKA DAMAI KECAMATAN PLAKAT TINGGI KABUPATEN MUSI BANYUASINIswinda Pramita(1) Houtman(2) Zainal Abidin(3)Pendidikan Bahasa dan Sastra IndonesiaUniversitas PGRI Palembangiswindap@gmail.com(1)Â Â ABSTRAK Penelitian ini membahas tentang pemertahanan bahasa Musi di desa Suka Damai Kecamatan Plakat Tinggi. Masalah dalam penelitian ini adalah bagaimana pemertahanan bahasa Musi di desa Suka Damai KecamatanÂ  Plakat Tinggi. Penelitian ini bertujuan untuk mendeskripsikan tentang pemertahanan bahasaÂ  Musi di desa Suka Damai Kecamatan Plakat Tinggi Kabupaten Musi Banyuasin. Metode yang digunakan dalam penelitian ini adalah metode kualitatif deskriptif. Data yang diperoleh dari 2 orang informan. Teknik pengumpulan data yang digunakan dalam penelitian ini adalah teknik observasi, teknik angket dan teknik wawancara. Adapun objek yang digunakan dalam penelitian ini masyarakat yang ada di desa Suka Damai Kecamatan Plakat Tinggi Kabupaten Musi Banyuasin. Hasil penelitian ini menunjukan bahwa bahasa Musi merupakan bahasa yang digunakan oleh penuturnya di desa Suka Damai Kecamatan Plakat Tinggi Kabupaten Musi Banyuasin. Dalam situasi informal penggunaan bahasa Musi terjadi, sedangkan dalam situasi formal masyarakat Musi sebagian menggunakan bahasa Indonesia . kontak bahasa terjadi yaitu antara bahasa Musi dan bahasa Jawa. Dari penelitian ini dapat disimpulkan bahwa masyarakat desa Suka Damai Kecamatan Plakat Tinggi Kabupaten Musi Banyuasin tetap mempertahankan bahasa MusiÂ  dengan cara mengajarkan bahasa Musi kepada penerus berikutnya.Kata Kunci : Pemertahanan Bahasa MusiMUSI LANGUAGE MAINTENANCE IN SUKA DAMAI VILLAGE, PLAKAT TINGGI DISTRICTÂ MUSI BANYUASIN DISTRICTÂ ABSTRACTThis study discusses the maintenance of the Musi language in Suka Damai village, Plakat Tinggi District. The problem in this study is how to maintain Musi language in Suka Damai village, Plakat Tinggi DistrictThis study aims to describe the Musi language retention in Suka Damai village, Plakat Tinggi District, Musi Banyuasin Regency. The method used in this research is descriptive qualitative method. Data obtained from 2 informants. Data collection techniques used in this study were observation techniques, questionnaire techniques and interview techniques. The objects used in this study are the people in Suka Damai village, Plakat Tinggi District, Musi Banyuasin Regency. The results of this study indicate that Musi language is the language used by speakers in Suka Damai village, Plakat Tinggi District, Musi Banyuasin Regency. In an informal situation the use of the Musi language occurs, whereas in a formal situation the Musi community partly uses Indonesian. Language contact occurs between Musi language and Javanese. From this research, it can be concluded that the people of Suka Damai village, Plakat Tinggi Subdistrict, Musi Banyuasin Regency still maintain Musi language by teaching Musi language to the next successor.Keywords: Musi Language Retention
KAJIAN INTERTEKSTUAL ANTARA NOVEL DILAN 1991 KARYA PIDI BAIQ DENGAN NOVEL DELUSI KARYA SIRHAYANI
Authors: Wijayanty Meika Tyara
Published: September 2023

KAJIAN INTERTEKSTUAL ANTARA NOVEL DILAN 1991 KARYA PIDI BAIQ DENGAN NOVEL DELUSI KARYA SIRHAYANIÂ Meika Tyara Wijayanty(1), Liza Murniviyanti(2), Barkudin(3)Universitas PGRI PalembangMeikatyara@yahoo.comÂ Â AbstrakKajian intertekstual merupakan hubungan antara teks satu dengan teks yang lainnya. Masalah penelitian ini adalah â€œbagaimanakah kajian intertekstual antara novel Dilan 1991 karya Pidi Baiq dengan novel Delusi karya Sirhayani. Tujuan penelitian ini adalah untuk mengetahui persamaan maupun perbedaan tema, penokohan, alur dan sudut pandang yang terdapat di dalam novel. Sumber data yang digunakan adalah novel Dilan 1991 karya Pidi Baiq dengan novel Delusi karya Sirhayani. Teknik yang digunakan dalam penelitian ini adalah teknik analisis isi. Berdasarkan hasil kajian dan pembahasan dapat disimpulkan bahwa antara novel Dilan 1991 karya Pidi Baiq dan novel Delusi karya Sirhayani memiliki kesamaan tema terdapat perbedaan alur cerita terdapat persamaan penokohan terdapat perbedaan sudut pandang. Perbedaan penokohan dari kedua novel ini adalah penokohan yang ditampilkan di dalam bentuk yang berbeda, karakter tokoh-tokoh tambahan yang digambarkan berbeda-beda dan sama-sama menggunakan alur campuran. Setelah itu munculnya konflik diantara novel tersebut dan terdapat sudut pandang yang berbeda yaitu novel Dilan 1991 karya Pidi baiq menggunakan sudut pandang orang pertama â€œAkuâ€. Sedangkan novel Delusi karya Sirhayani menggunakan sudut pandang orang ketiga â€œDiaâ€.Â Kata Kunci: Intertekstual, Novel, Â Dilan dan Delusi Â INTERTEXTUAL STUDY BETWEEN NOVEL DILAN 1991 BY PIDI BAIQ AND NOVEL DELUSI BY SIRHAYANIÂ AbstractIntertextual study is the relationship between one text and another. The problem of this research is "how is the intertextual study between the 1991 Dilan novel by Pidi Baiq and the novel Delusi by Sirhayani. The purpose of this study was to determine the similarities and differences in the themes, characterizations, plot and points of view contained in the novel. The data source used is the Dilan 1991 novel by Pidi Baiq with the novel Delusi by Sirhayani. The technique used in this research is content analysis technique. Based on the results of the study and discussion, it can be concluded that between the 1991 Dilan novel by Pidi Baiq and the novel Delusi by Sirhayani, there are similar themes, there are differences in storylines, there are similarities in characterization, there are different points of view. The difference in characterization of the two novels is that the characters are shown in different forms, the characters of the additional characters are depicted differently and both use a mixed plot. After that conflict arose between the novels and there was a different point of view, namely the 1991 Dilan novel by Pidi Baiq using the first person perspective "I". Meanwhile, Sirhayani's novel Delusi uses a third person perspective "Dia".Keywords: Intertextual, Novel, Dilan and Delusion
Between the System and the Opposition: The Evolution of the Electoral Portrait of the National Rally, 2017–2022
Authors: Zhidkova Aleksandra
Published: September 2023

The French electoral cycle that ended in 2022 proved that far-right leader Marine Le Pen has succeeded in establishing herself as the recognised standard-bearer of the French opposition. The success of the 2022 elections was another milestone in the successive electoral ascent of the Rassemblement Nationale: in the 2012 presidential election, Marine Le Pen obtained 17.9% of the vote, 21% in 2017, and in 2022 she received 23% in the first round and 41% in the second. This expansion of the electoral base was accompanied by a change in the party's electoral profile, as evidenced by the sociological research that was the main source for this article. First of all, the author demonstrates which social categories were responsible for the rise in Le Pen's popularity and how the party's rhetoric and, at times, policy positions were transformed in response to the mood of voters. She also analyses the correlation between the unstable socio-economic situation and the strengthening of the extreme right, suggesting the extent to which the vote for Le Pen is influenced not only by the financial and social status of the voter, but also by the anxiety triggered by worsening economic issues and the mainstreaming of immigration and security issues. Finally, an examination of the values of Le Pen's electorate allows one to look at the perspectives and constraints of the “Marinism” strategy in a broader context.
THE ACTIVITY OF HEME OXYGENASE AND δ-AMINOLEVULINATE SYNTHASE IN THE RAT KIDNEY UNDER CONDITIONS OF TOXIC INJURY WITH ACETAMINOPHEN
Authors: Voloshchuk O.M. , Moskaliuk N.O.
Published: September 2023

The aim of this work was to study the activity of δ-aminolevulinate synthase, heme oxygenase and the content of mitochondrial cytochromes in the rat kidney conditions of toxic damage with acetaminophen against the background of protein deficiency. δ-Aminolevulinate synthase activity was determined spectrophotometrically taking into account the molar extinction coefficient of 0.023·103 mol-1·cm-1, heme oxygenase activity – by the amount of bilirubin formed and expressed in nanomoles per 1 mg of protein per 1 minute. The determination of the contents of cytochromes a+a3, b, c and c1 in the suspension of isolated mitochondria was carried out by the difference spectra between the reduced and oxidized states in the region of their spectral maxima. The study was conducted on 4 groups of animals: Group I - control animals (K); II – rats kept on a low-protein diet (LPD); ІІІ – animals with acetaminophen-induced liver injury receiving complete ration (AII); IV – animals with acetaminophen-induced liver injury that were previously maintained on semi-synthetic low-protein ration (LPD/AII). It was established that under the conditions of consumption of a low-protein diet, there are no significant changes in the activities of heme oxygenase and δ-aminolevulinate synthase, but a decrease in the content of mitochondrial cytochromes a+a3, b, c, c1 is observed, which probably indicates the critical role of providing protein in the diet for the synthesis of cytochromes. At the same time, under the conditions of toxic damage by acetaminophen, regardless of whether the ration is provided with protein, there is a decrease in the activity of δ-aminolevulinate synthase by an average of 1.6 times with a simultaneous increase in the activity of heme oxygenase by approximately 1.5 times compared to the control values, which indicates an increase in the processes of heme catabolism on against the background of slowing down of its synthesis under the studied conditions. The increase in the activity of heme oxygenase against the background of the decrease in the activity of δ-aminolevulinate synthase can be considered as one of the possible mechanisms of the established decrease in the content of mitochondrial cytochromes under the conditions of acetaminophen intoxication, which in turn may further lead to a violation of the activity of the enzymes of the cytochrome part of the respiratory chain. The obtained results regarding the content of cytochromes, the activities of key enzymes of heme metabolism in the kidneys can be considered as prerequisites for deepening the energy imbalance under the conditions of toxic damage to the kidneys by acetaminophen.
THE EXPERIMENTAL DETERMINATION OF THE NUMBER OF FREEZING STAGES OF CANDIDA TROPICALIS FUNGAL CELLS FOR THEIR DESTRUCTION AND OBTAINING OF PROTEINS AND POLYSACCHARIDES
Authors: Rybalkin M.V. , Strilets O.P. , Strelnikov L.S.
Published: September 2023

Candidiasis is a disease that occurs due to excessive growth of the Candida fungus. Several types of this pathogen are known, each of which can cause damage to various organs and systems. Recently, there have been reports of the loss of sensitivity of Candida fungi to most of antifungal drugs that have been used for more than 40 years. An alternative to antifungal drugs for candidiasis can be the use of a vaccine for the prevention and treatment of candidal infection. In our opinion, it is promising to use a candidal vaccine based on a subunit vaccine that contains fragments of a microorganism without ballast substances. Cell fragments of Candida fungi that have antigenic properties include proteins and polysaccharides. To isolate antigenic substances from Candida fungal cells, we chose freezing as the method of destruction. The aim of this work was to experimentally substantiate the number of freezing stages for the destruction of C. tropicalis АТСС 20336 fungi cells and the subsequent production of proteins and polysaccharides. To determine the optimal number of freezing stages in the temperature range from (25 ± 2)°С to (-25 ± 2)°С, in order to destroy the cells of C. tropicalis fungi, the studies were conducted with freezing stages 3, 4, 5, and 6. Centrifugation was used to separate the excess components. Then the preliminary and sterilizing filtration was performed on membrane filters with pore diameters of 0.45 μm and 0.22 μm. The content of protein, polysaccharides and monosaccharides were determined in each case. The protein content was determined according to the State Pharmacopoeia of Ukraine (SPhU). To determine polysaccharides, a reaction with phenol and sulfuric acid was performed. Chromatographic studies of monosaccharides were conducted using paper chromatography according to the SPhU. Based on the results of the research, it was found that solutions obtained at stages 5 and 6 of freezing the biomass of C. tropicalis fungi cells at a temperature from (25 ± 2) °C to (-25 ± 2) °C contained the highest amount of proteins and polysaccharides. It is likely that with these number of stages for freezing the biomass of C. tropicalis fungi cells, active substances are released from all layers of Candida fungi cells. Thus, it can be concluded that five stages of freezing are the optimal number that ensures the maximum extraction of polysaccharides and proteins quickly and economically.
APPLICATION OF BIOSURFACTANTS IN WASTEWATER BIOFILTRATION TECHNOLOGY USING DAPHNIA MAGNA
Authors: Khuda L.V. , Frunza O.E. , Karpenko O.V. , Lubenets V.I. , Khudyi O.O.
Published: September 2023

When solving water purification problems, including for the needs of industrial fish farming, biofiltration methods involving planktonic organisms, in particular branchiopod crustaceans, deserve special attention. A specific filtration apparatus feeding daphnia can provide highly efficient wastewater treatment from suspended fine particles that have a low settling velocity and are not able to be retained on mechanical filters. An important advantage of this method of water treatment is the ability to use the resulting zooplankton biomass as live feed for fish farming. The filtration properties of zooplankton can be improved by growing it in the presence of surfactants of biological origin, which, in addition, improve the absorption of nutrients by cladocerans and intensify their growth. In this study, the possibility of using a preparation of trehalosolipid biosurfactants obtained from the culture fluid of Rhodococcus erythropolis AU-1 for intensive growth of Daphnia magna culture for the purpose of biological treatment of water bodies was evaluated. It was established that the toxicity of trehalosolipids is dose-dependent, in particular, their minimum concentration at which the death of daphnia was observed within 24 hours of exposure was 300 mg/l, and the LC50 at the same duration of exposure was 479 mg/l. The maximum non-toxic concentration of the trehalosolipid biosurfactant is 200 mg/l. Concentrations of trehalosolipids in the range of 1-3 mg/l provide an increase in the density of daphnia culture by 1.3-1.7 times compared to the control. To evaluate the efficiency of biofiltration of Daphnia magna in the model experiment, water from the mechanical filter of the experimental recirculation system of the Yuriy Fedkovych Chernivtsi National University was used. The value of the optical density of the discharge water decreases by more than half after one day of daphnia presence in it, and after 4 days - by 7 times. The addition of biosurfactants leads to a more efficient filtration process, with the best results obtained when using the preparation with a concentration of 1 mg/l. The use of biosurfactants in concentrations of 1, 2 and 3 mg/l leads to a decrease in electrical conductivity and total mineralization compared to the control. At the same time, the pH and soluble oxygen content did not change compared to the control.
ORGANIZATION OF THE 5S RIBOSOMAL DNA INTERGENIC SPACER OF APAMEA MONOGLYPHA (LEPIDOPTERA)
Authors: Roshka N.M.
Published: September 2023

A significant part of the moth species, representatives of the superfamily Noctuoidea distributed on the territory of Ukraine, are pests of crops, which attracts special attention of researchers. In particular, host plants for the larvae of the Apamea species are cereals. For the correct identification of the pest moth speciesit is necessary to conduct molecular genetic studies to create molecular passports (barcoding). One of the convenient molecular markers used to compare low-ranking taxa is the 5S rDNA region represented by tandemly arranged clusters, where each repeat unit consists of a highly conserved coding region and a variable intergenic spacer (IGS). Since the molecular organization of 5S rDNA in moth species is still insufficiently studied, the aim of our work was to analyze the 5S rDNA IGS of A. monoglypha, a representative of the Apamea genus. For this, 5S rDNA repeats were amplified by PCR, and the obtained products were ligated into the pJet1.2 plasmid vector. The inserts of the three recombinant plasmids, ApMon1-1, ApMon1-2, and ApMon1-8, which were of the expected size, were sequenced. It was found that the A. monoglypha genome contains several structural IGS variants that differ both in length and nucleotide sequence. The level of similarity of the compared IGS sequences varied from 38.0 to 76.2%, indicating a high level of intragenomic polymorphism in this region. An AT-rich motif, a GC dinucleotide, and a cytosine residue up-stream of the coding region were identified as putative external promoter elements, which can be involved in the initiation of transcription. Furthermore, the oligo-T motif located immediately down-stream of the coding region is probably involved in the transcription termination.
JOINT CULTIVATION OF REPRESENTATIVES OF THE GENERA PROPIONIBACTERIUM, LACTOBACILLUS, BIFIDOBACTERIUM
ON UNIFIED MEDIA
Authors: Vasina L.M. , Shtohryn N.A.
Published: September 2023

Probiotic microorganisms are living non-pathogenic, non-toxigenic microorganisms that enter the intestine with food and have a positive effect on the body, normalising the composition and biological activity of the microflora of the digestive tract. Lactic acid bacteria of the genera Lactobacillus and Bifidobacterium occupy a prominent place among the microorganisms that make up probiotics. The presence of these bacteria in the gastrointestinal tract provides an antagonistic environment that is directed against pathogenic microorganisms and creates conditions for the growth and development of beneficial bacteria. Bifidobacterium and Lactobacillus can enhance the absorption capacity of food components, lactose tolerance, stabilise liver function by normalising bowel function and reducing blood ammonia levels.
Recently, the spectrum of microorganisms that exhibit antagonistic properties and have a favourable effect on the vital activity of the organism has been expanding. Much attention is paid to propionic acid bacteria, which are part of the microflora of various biotopes. Propionic acid bacteria synthesise vitamin B12, bifidogenic growth factors and bacteriocins, have high adhesive and immunomodulatory properties, and their metabolites have antimutagenic effects. In this study, we analysed the possibility of individual and joint cultivation of representatives of the genera Propionibacterium, Lactobacillus and Bifidobacterium on unified media containing corn and yeast extracts. It was found that these nutrient media are effective for monocultivation of propionic acid bacteria and lactobacilli. At the same time, not only an increase in the optical density of propionic acid bacteria (3-fold), but also an increase in acid formation (2-fold on average) was observed. The possibility of joint cultivation of lactic acid and propionic acid bacteria by creating associations of two types was proved. The number of colonies of the studied microorganisms on selective nutrient media after cocultivation in unified media of both types was determined, the highest survival rate was observed for Propionibacterium. The antagonistic effect of both individual cultures and established associations against certain gram-positive bacteria and microscopic fungi was recorded.
EVALUATION OF THE EFFICIENCY AND PROSPECTS OF THE USE OF IRON NANOAQUACHELATE IN VITRO PLANT CULTURE
Authors: Subin O.V. , Klyuvadenko A.A. , Lobova O.V. , Likhanov A.F.
Published: September 2023

The paper presents studies of the effect of nanoaquachelate of iron on the growth processes of tobacco plants-regenerants in in vitro plant culture. Morphological features of Nicotiana tabacum L. shoots were compared with the use of FeEDTA, in conditions of its deficiency, and with the use of nanoaquachelate solutions. The peculiarities of the development of regenerating plants of tobacco in conditions of iron deficiency are shown.
It was established that under the conditions of long-term cultivation (28 days) with the use of iron nanoaquachelate at a concentration of 140 μg/ml, regenerating plants did not show any signs of inhibition of vital activity, chlorotic depigmentation or aging compared to the control.
Anatomical and histochemical features of regenerating plants of tobacco, which were cultivated on nutrient media with different forms and iron content, are shown.
AN ALGORITHM FOR RESEARCH OF SUNFLOWER HYBRIDS PRODUCTIVITY
UNDER CONDITIONS OF INSECT POLLINATION
Authors: Fedoriak M.M. , Zhuk A.V. , Zarochentseva O.D. , Fylypchuk T.V. , Sytnikova I.O. , Leheta U.V. , Moskalyk H.G. , Herasymuk P.V. , Sosnovskyi K.S. , Shpak Y.V.
Published: September 2023

The article summarizes the practical experience of five-year research on seed productivity of autofertile hybrids of sunflower Helianthus annuus L. in the presence and absence of insect pollination. The productivity of 21 sunflower hybrids from six producers «Pioneer», «Syngenta», «Euralis», «Limagrain», «MAS Seeds», «RAGT» - was studied. Experiments were conducted in the fields of the Continental Farmers Group agro-holding in the agroclimatic conditions of Western Ukraine.
To exclude the effect of insect pollination, the heads of experimental plants were covered with bag isolators made of 50x40 cm polypropylene spunbond with a density of 30 g/m2 and the ability to adjust the strength of tying during inflorescence development. The heads of control plants were left open for free insect pollinators access. After full seed maturity, the isolators were removed from the experimental plants, and the heads of control plants were selected based on the identity of their diameters. The selected material was dried to an air-dry state and used for further research in laboratory conditions.
Seed productivity was determined by indicators such as incompleteness of heads and seeds in different zones of the head disk, huskiness, 1000-seed weight, purity, biological yield, oil content, and protein content. Individual indicators of hybrid productivity were studied for the head disk within the outer, intermediate, and inner zones, each of which occupied one-third of its radius. To determine the incompleteness of seeds and huskiness in each zone of the head disk, 20 seeds were randomly selected from each zone and cleaned of seed coats. Analysis of oil content and protein content was carried out by a non-invasive method of NIR spectroscopy.
The paired Wilcoxon criterion was used to determine statistically significant differences between control and experimental groups of plants under different pollination conditions.
As a result of the conducted research, it was possible to develop an algorithm for determining quantitative and qualitative indicators of productivity of modern sunflower hybrids in the presence and absence of insect pollination
RACES COMPOSITION OF THE COLONIES OF HONEY BEE (APIS MELLIFERA L.) IN THE APIARIES OF THE NORTHERN BUKOVINA
Authors: Tymochko L.I. , Cherevatov O.V. , Cherevatov V.F.
Published: September 2023

Modern honey bee races have evolved from local natural subspecies. Due to this, native bees are adapted better to local climatic conditions and are less susceptible to the combined effects of negative factors that lead to colony extinction. However, the distribution of honey bee subspecies in Europe over the past century has been heavily influenced by humans. According to the plan of breed zoning of honey bees in Ukraine, Chernivtsi region is defined as the region of the Carpathian bee. However, interbreed hybrids were formed due to the uncontrolled introduction of other breeds to the area and spontaneous hybridisation between them and native bees. Due to the existence of such hybrid forms, the diversity of local bee ecotypes is lost and genetic polymorphism within the population increases dramatically. This increase in polymorphism has led to an increase in biodiversity, the emergence of numerous intermediate variants of morphometric characteristics, and the loss of valuable Carpathian bee traits. Modern phylogenetic studies of bees include the analysis of a set of morphometric external parameters: cubital index, discoidal displacement and proboscis length. An integrated approach to the study of these traits can reflect the evolution of honey bees and, accordingly, their belonging to specific subspecies or breeds. In the region as a whole, the predominant breeds were Carpathian, Ukrainian Steppe, and Grey Mountain Caucasian, which belong to the subspecies A. m. carnica (Carpathian ecotype), A. m. macedonica and A. m. caucasica, respectively. In addition, the hybrid forms contain features of the alpine variant of carnica and dark European bees (A. m. mellifera). It is shown that hybridisation within Northern Bukovina was chaotic, and no localities with preservation of Carpathian lines were found.
Clinical manifestations of non-compact left ventricular myocardium
Authors:   Northern State Medical University, IVANOVA S. N. , GALASHEVSKAYA L. A. ,   Arkhangelsk Regional Clinical Hospital, MARTYNOV V. O. ,   Northern State Medical University
Published: September 2023

The purpose — to present the data of a clinical case of non-compact left ventricular myocardium complicated by severe cardiac arrhythmias and heart failure. Material and methods. The diagnosis of non-compact myocardium of the left ventricle became possible since 1990s after the introduction of echocardiography as a diagnostic method. The disease genesis is still unclear, starting with clinical manifestations of complications: cardiac arrhythmia or heart failure or thromboembolism, leading to fatal outcomes. Conclusions. The article is of interest to practitioners, since non-compact myocardium of the left ventricle is a rare disease, which presumably has both a «family» and a «non-family» character, with the possible occurrence of a triad of syndromes or an asymptomatic course without sufficient evidence for the treatment of this pathology.
Headache in patients with connective tissue dysplasia
Authors:   Kazan State Medical Academy — Branch Campus of the FSBEI FPE RMACPE MOH Russia, MANSUROV D. M. , KHAIBULLINA D. KH. ,   Kazan State Medical Academy — Branch Campus of the FSBEI FPE RMACPE MOH Russia
Published: September 2023

Headache in patients with connective tissue dysplasia (CTD) is an urgent problem of modern medicine. The article discusses different types of headache in patients with connective tissue dysplasia. The most well studied are differentiated forms of connective tissue dysplasia (Ehlers — Danlos syndrome, Marfan syndrome, etc.) and, accordingly, variants of cephalgia in them are described. A significant problem is the dissection of the carotid and vertebral arteries, which are variants of acute cerebrovascular accident. The interrelation of primary and secondary headaches with CTD syndrome is shown. The variety of clinical manifestations of headaches and connective tissue dysplasia in the same patient makes it difficult to diagnose, which can contribute to the chronicity of cephalalgia, the appointment of inadequate therapy and the development of complications. Further study of headache options in patients with CTD will improve the provision of medical care to this category of patients.
Effectiveness of anticoagulants in the prevention of thrombotic complications in patients with cardiovascular pathology who have had COVID-19
Authors:   Ural State Medical University, KLYACHINA E. S. , SMOLENSKAYA O. G. ,   Ural State Medical University, VEDENSKAYA S. S. ,   Ural State Medical University
Published: September 2023

At the moment, there is a lot of data demonstrating the effectiveness of anticoagulants in the acute period of COVID-19; as for their effectiveness in patients after discharge, there are relatively few data, especially in patients with concomitant cardiological pathology. The purpose — is to evaluate the relationship of taking anticoagulants, within one month after discharge, with the development of cardiovascular events and deaths in patients with cardiovascular pathology during 3 months of follow-up. Material and methods. A retrospective, cohort, observational study of 317 patients who had COVID-19 and had a history (before COVID-19) of cardiovascular pathology was conducted. Results. Within three months after discharge from the hospital, 15 (4.73%) cardiovascular events were registered. The frequency of arterial thrombotic complications was 3.79% (n = 12), venous thrombotic complications 0.95% (n = 3). All cardiovascular events were reported in patients not using anticoagulants (n = 15; 6.28%), p = 0.026. When analyzing concomitant cardiovascular pathology in patients with registered events, it was revealed that the incidence of atrial fibrillation and ischemic stroke in their anamnesis was statistically significantly higher (33.33 and 40%), relative to the comparison group (10.03 and 7.9%; p = 0.002; p = 0.001, respectively). Conclusions. The use of anticoagulants in patients with cardiovascular pathology during treatment in a COVID hospital and within one month after discharge was associated with a significant decrease in thromboembolic events over 3 months of follow-up. Predictors of thromboembolic events in patients with cardiovascular pathology and those who have had COVID-19 are: senior age; atrial fibrillation and ischemic stroke in the anamnesis.
Wskazania dla polityków w nauczaniu społecznym Jana Pawła II
Authors: Krukowski Józef
Published: September 2023

Jest to wykład, który został wygłoszony w Sejmie RP 15 kwietnia 2011 r. z okazji beatyfikacji Jana Pawła II. Wykład obejmuje dwie grupy zagadnień. Pierwsza grupa dotyczy prerogatyw, w których głos w sprawach polityki zabierał papież Jan Paweł II. Druga grupa obejmuje główne wątki problemowe w jego licznych wystąpieniach skierowanych do polityków. Papież zwrócił uwagę, że politycy w swoich działaniach powinni kierować się zasadą dobra wspólnego człowieka, zasadą pomocniczości, solidarności, sprawiedliwości i ochroną praw człowieka.
The European Union Law of and Christian Values
Authors: Krukowski Józef
Published: September 2023

The Author explained the term of the law and the term of the values, especially Christian values. In the article, the Author distinguished two categories of the Christian values: 1) peculiar Christian values based on the theological grounds singling out religion and Christian ethic. The Church regulates the means of the achievement of these values with the assistance of the canon law; 2) universal ethical principles, which are basic human values, such as respect for the hierarchy of values, recognition that human being is in the first place in that hierarchy and recognition that the natural human dignity is a source of the fundamental rights and freedoms. The law of the European Union refers to the values. Politicians of the European Union have made the project of building the community called “European civil society”. Christians as the citizens of the European Union, should not be just passive observers while basic universal Christian values are disregarded. They should demand from politicians to respect the principle of the common good.
The Obligation of the Faithful to Remain in Obedience to the Roman Pontiff and the Bishops in Communion with Him (Cann. 204 & 205). An Outline
Authors: Sitarz Mirosław
Published: September 2023

The Author presents the third element of the full communion with the Church - ecclesiastical governance - namely the obligation of the faithful to remain in obedience to the Roman Pontiff and the bishops in communion with him. First, the definition of obedience, its theological and legal foundations and subject (recipient) of obedience were explained in the article. The Author, with regard to its subject, specified the following kinds of obedience: 1) all the faithful, 2) clerical persons, and 3) consecrated religious. With regard to the scope of obedience, it is required of: 1) absolute (that is bound with authentic and definitive teaching on faith and morality), 2) religious (that is bound with authentic but not definitive teaching), and 3) canonical (that is bound with maintaining ecclesiastical discipline).
Safety, Tolerability, and Pharmacokinetics of Zagotenemab in Participants with Symptomatic Alzheimer’s Disease: A Phase I Clinical Trial
Authors: Willis Brian A. , Lo Albert C. , Dage Jeffrey L. , Shcherbinin Sergey , Chinchen Louise , Andersen Scott W. , LaBell Elizabeth S. , Perahia David G.S. , Hauck Paula M. , Lowe Stephen L.
Published: September 2023

Background:

Zagotenemab (LY3303560), a monoclonal antibody, preferentially binds to extracellular, misfolded, aggregated tau that has been implicated in Alzheimer’s disease (AD).

Objective:

The goal of this study was to assess the safety and pharmacokinetics of multiple doses of zagotenemab in participants with AD.

Methods:

This was a Phase Ib, multi-site, participant- and investigator-blind, placebo-controlled, parallel-group study in participants with mild cognitive impairment due to AD or mild to moderate AD. After screening, participants were randomized to zagotenemab 70 mg, 210 mg, or placebo every 4 weeks for up to 49 weeks and were followed up for 16 weeks.

Results:

A total of 13 males and 9 females, aged 59 to 84 years, were dosed. No deaths occurred during this study. A total of 4 serious adverse events occurred in 2 participants who then discontinued the study. The most commonly reported (3 or more participants) treatment-emergent adverse events were sinus bradycardia, headache, fall, and bronchitis. The pharmacokinetics profile showed generally linear exposures across the dose range studied with a clearance of ~8 mL/h. The half-life of zagotenemab in serum was ~20 days. A dose-dependent increase in plasma tau was observed. No other significant pharmacodynamic differences were observed due to low dose levels and limited treatment duration.

Conclusions:

No dose-limiting adverse events were observed with zagotenemab treatment. Pharmacokinetics of zagotenemab were typical for a monoclonal antibody. Meaningful pharmacodynamic differences were not observed. Clinicaltrials.gov: NCT03019536
Global regularity for Oldroyd-B model with only stress tensor dissipation
Authors: Feng Weixun , Chen Zhi , Qin Dongdong , Tang Xianhua
Published: September 2023

In this paper, we consider the d-dimensional ([Formula: see text]) Oldroyd-B model with only dissipation in the equation of stress tensor, and establish a small data global well-posedness result in critical [Formula: see text] framework. Particularly, we give a positive answer to the problem proposed recently by Wu-Zhao ( J. Differ. Equ. 316 ( 2022 )) involving the upper bound for the time integral of the lower frequency piece of the stress tensor, and show that it is indeed independent of the time. Moreover, we improve the results in ( J. Math. Fluid Mech. 24 ( 2022 )) by relaxing the space dimension [Formula: see text] to any [Formula: see text].
Identification of molecular biomarkers associated with non-small-cell lung carcinoma (NSCLC) using whole-exome sequencing
Authors: Singh Varsha , Katiyar Amit , Malik Prabhat , Kumar Sunil , Mohan Anant , Singh Harpreet , Jain Deepali
Published: September 2023

OBJECTIVES: Significant progress has been made in the treatment of patients with pulmonary adenocarcinoma (ADCA) based on molecular profiling. However, no such molecular target exists for squamous cell carcinoma (SQCC). An exome sequence may provide new markers for personalized medicine for lung cancer patients of all subtypes. The current study aims to discover new genetic markers that can be used as universal biomarkers for non-small cell lung cancer (NSCLC). METHODS: WES of 19 advanced NSCLC patients (10 ADCA and 9 SQCC) was performed using Illumina HiSeq 2000. Variant calling was performed using GATK HaplotypeCaller and then the impacts of variants on protein structure or function were predicted using SnpEff and ANNOVAR. The clinical impact of somatic variants in cancer was assessed using cancer archives. Somatic variants were further prioritized using a knowledge-driven variant interpretation approach. Sanger sequencing was used to validate functionally important variants. RESULTS: We identified 24 rare single-nucleotide variants (SNVs) including 17 non-synonymous SNVs, and 7 INDELs in 18 genes possibly linked to lung carcinoma. Variants were classified as known somatic (n= 10), deleterious (n= 8), and variant of uncertain significance (n= 6). We found TBP and MPRIP genes exclusively associated with ADCA subtypes, FBOX6 with SQCC subtypes and GPRIN2, KCNJ18 and TEKT4 genes mutated in all the patients. The Sanger sequencing of 10 high-confidence somatic SNVs showed 100% concordance in 7 genes, and 80% concordance in the remaining 3 genes. CONCLUSIONS: Our bioinformatics analysis identified KCNJ18, GPRIN2, TEKT4, HRNR, FOLR3, ESSRA, CTBP2, MPRIP, TBP, and FBXO6 may contribute to progression in NSCLC and could be used as new biomarkers for the treatment. The mechanism by which GPRIN2, KCNJ12, and TEKT4 contribute to tumorigenesis is unclear, but our results suggest they may play an important role in NSCLC and it is worth investigating in future.