bluuebunny/crossref_metadata_2025 huggingface dataset:
Load Frequency Control Using Hybrid Intelligent Optimization Technique for Multi-Source Power Systems
Authors: Gupta Deepak Kumar , Jha Amitkumar V. , Appasani Bhargav , Srinivasulu Avireni , Bizon Nicu , Thounthong Phatiphat
Published: March 2021

The automatic load frequency control for multi-area power systems has been a challenging task for power system engineers. The complexity of this task further increases with the incorporation of multiple sources of power generation. For multi-source power system, this paper presents a new heuristic-based hybrid optimization technique to achieve the objective of automatic load frequency control. In particular, the proposed optimization technique regulates the frequency deviation and the tie-line power in multi-source power system. The proposed optimization technique uses the main features of three different optimization techniques, namely, the Firefly Algorithm (FA), the Particle Swarm Optimization (PSO), and the Gravitational Search Algorithm (GSA). The proposed algorithm was used to tune the parameters of a Proportional Integral Derivative (PID) controller to achieve the automatic load frequency control of the multi-source power system. The integral time absolute error was used as the objective function. Moreover, the controller was also tuned to ensure that the tie-line power and the frequency of the multi-source power system were within the acceptable limits. A two-area power system was designed using MATLAB-Simulink tool, consisting of three types of power sources, viz., thermal power plant, hydro power plant, and gas-turbine power plant. The overall efficacy of the proposed algorithm was tested for two different case studies. In the first case study, both the areas were subjected to a load increment of 0.01 p.u. In the second case, the two areas were subjected to different load increments of 0.03 p.u and 0.02 p.u, respectively. Furthermore, the settling time and the peak overshoot were considered to measure the effect on the frequency deviation and on the tie-line response. For the first case study, the settling times for the frequency deviation in area-1, the frequency deviation in area-2, and the tie-line power flow were 8.5 s, 5.5 s, and 3.0 s, respectively. In comparison, these values were 8.7 s, 6.1 s, and 5.5 s, using PSO; 8.7 s, 7.2 s, and 6.5 s, using FA; and 9.0 s, 8.0 s, and 11.0 s using GSA. Similarly, for case study II, these values were: 5.5 s, 5.6 s, and 5.1 s, using the proposed algorithm; 6.2 s, 6.3 s, and 5.3 s, using PSO; 7.0 s, 6.5 s, and 10.0 s, using FA; and 8.5 s, 7.5 s, and 12.0 s, using GSA. Thus, the proposed algorithm performed better than the other techniques.
Synergistic Catalytic Effect of Sulphated Zirconia—HCl System for Levulinic Acid and Solid Residue Production Using Microwave Irradiation
Authors: Hurst George , González-Carballo Juan Maria , Tosheva Lubomira , Tedesco Silvia
Published: March 2021

The synergistic conversion of Miscanthus xGiganteous with sulphated zirconia and dilute hydrochloric acid was investigated. The sulphated zirconia was prepared using H2SO4 impregnation and characterised using X-ray Diffraction (XRD), Energy-dispersive X-ray (EDX), Scanning Electron Miscroscope (SEM) spectroscopy and nitrogen adsorption–desorption measurements. The microwave-assisted reaction was evaluated at various temperatures, reaction times and catalyst-to-biomass ratios, with and without the presence of trace HCl in the solution medium for the conversion of Miscanthus xGiganteous to levulinic acid. The highest levulinic acid yield of 63.8% was achieved at 160 °C, 80 min and a 2:1 catalyst-to-biomass ratio, with 10 mM HCl. The catalyst recyclability was investigated with and without calcination, finding that significant humin deposition on the catalyst surface likely caused catalyst deactivation. The post-reaction solid residue was also characterised using SEM, EDX, XRD, elemental composition and nitrogen adsorption–desorption measurements. Findings indicate that this residue could potentially be used as a soil amendment or as a fuel source. The synergistic conversion of real lignocellulosic biomass with sulphated zirconia and trace hydrochloric acid showed remarkable promise and should be investigated further.
Development of Three-Phase Permanent-Magnet Synchronous Motor Drive with Strategy to Suppress Harmonic Current
Authors: Kao Wei-Tse , Hwang Jonq-Chin , Liu Jia-En
Published: March 2021

This study aimed to develop a three-phase permanent-magnet synchronous motor drive system with improvement in current harmonics. Considering the harmonic components in the induced electromotive force of a permanent-magnet synchronous motor, the offline response of the induced electromotive force (EMF) was measured for fast Fourier analysis, the main harmonic components were obtained, and the voltage required to reduce the current harmonic components in the corresponding direct (d-axis) and quadrature (q-axis) axes was calculated. In the closed-loop control of the direct axis and quadrature axis current in the rotor reference frame, the compensation amount of the induced EMF with harmonic components was added. Compared with the online adjustment of current harmonic injection, this simplifies the control strategy. The drive system used a 32-bit digital signal processor (DSP) TMS320F28069 as the control core, the control strategies were implemented in software, and a resolver with a resolver-to-digital converter (RDC) was used for the feedback of angular position and speed. The actual measurement results of the current harmonic improvement control show that the total harmonic distortion of the three-phase current was reduced from 5.30% to 2.31%, and the electromagnetic torque ripple was reduced from 15.28% to 5.98%. The actual measurement results verify the feasibility of this method.
Heavy Metals Behavior in Soil/Plant System after Sewage Sludge Application
Authors: Wydro Urszula , Jabłońska-Trypuć Agata , Hawrylik Eliza , Butarewicz Andrzej , Rodziewicz Joanna , Janczukowicz Wojciech , Wołejko Elżbieta
Published: March 2021

One of the possibilities of removing heavy metals (HMs) from soil is the use of phytoremediation techniques supported with biosolids, which also allow for their disposal. Therefore, the objective of the research was the determination of the sewage sludge suitability after its application to urban soil in order to increase the phytoremediation efficiency of contaminated soil. A field experiment was established on lawns in Białystok (Poland) in two locations with different traffic. The research plots were fertilized with sludge in doses of 14.5 t DM/ha and 29 t DM/ha. A mixture of lawn grasses was sown on the prepared plots. During two years of experiment soil/plant samples were collected, and pH, organic matter, dehydrogenase and catalase activity (soil), the total content of Cd, Cr, Cu, Mo, Ni, Pb, Zn, and Hg (soil/plant), and their fractions (soil) were determined. The HMs in soil were present mainly in residual and reducible fractions. Zn had the highest share in acid-soluble fractions (17–45%). The efficiency of urban soil phytoremediation was determined by the calculation of bioconcentration (BCF) and translocation (TF) factors. The highest values for BCF and TF were obtained for Mo (1.97 and 1.99, respectively). In the presented study, sludge amendment caused an immobilization of heavy metals.
Nonlinear Modelling, Flatness-Based Current Control, and Torque Ripple Compensation for Interior Permanent Magnet Synchronous Machines
Authors: Veeser Felix , Braun Tristan , Kiltz Lothar , Reuter Johannes
Published: March 2021

A nonlinear mathematical model for the dynamics of permanent magnet synchronous machines with interior magnets is discussed. The model of the current dynamics captures saturation and dependency on the rotor angle. Based on the model, a flatness-based field-oriented closed-loop controller and a feed-forward compensation of torque ripples are derived. Effectiveness and robustness of the proposed algorithms are demonstrated by simulation results.
Genome-Wide Characterization and Evolutionary Analyses of Purple Acid Phosphatase (PAP) Gene Family with Their Expression Profiles in Response to Low Phosphorus Stresses in Moso Bamboo (Phyllostachys edulis)
Authors: Zhou Mengyan , Chen Wanting , Zhao Mingzhen , Li Yachao , Li Ming , Hu Xia
Published: March 2021

Low phosphorus increases acid phosphatase activity and transfers soluble phosphorus from the underground to the above-ground, but also inhibits the growth and development of the Moso bamboo root system. Purple acid phosphatase (PAP), a kind of acid phosphatase, plays an important role in phosphorus (P) uptake and metabolism. In our study of the Moso bamboo PAP gene family, we identified 17 Moso bamboo PAP genes (PePAP) in the entire genome and further analyzed their physical and chemical properties and functions PePAP. According to the analysis of the phylogenetic tree, special domains and conserved motifs, these 17 genes can be divided into four categories. The gene structure and conserved motifs are relatively conservative, but the 17 sequences of the PePAP domain are diverse. The prediction of the subcellular location indicated that PePAPs are mainly located in the secretory pathway. We have studied the expression levels of these PePAP in different organs, such as the roots, stems and leaves of Moso bamboo, and the results show that the expression of most PePAP genes in roots and stems seems to be higher than that in leaves. In addition to tissue-specific expression analysis, we also studied the expression of PePAPs under low phosphorus stress. Under such conditions, the PePAP genes show an increase in expression in the roots, stem and leaves, and the extent of this change varies between genes. In summary, our results reveal the evolution of the PePAP gene in the Moso bamboo genome and provide a basis for understanding the molecular mechanism of the PePAP-mediated response of Moso bamboo to low phosphorus.
On the Origin of the Magnetic Concentration Gradient Force and Its Interaction Mechanisms with Mass Transfer in Paramagnetic Electrolytes
Authors: Waskaas Magne
Published: March 2021

The objective of this work is to analyze the origin of the magnetic concentration gradient force. The force will be studied in a diffusion system where a paramagnetic electrolyte diffuses through a thin, inert membrane under the influence of a homogeneous magnetic field. The force will be analyzed using the theory of magnetic circuits, i.e., by the concept of minimum reluctance principles. In addition, based on some previous studies, it will be discussed whether the minimum reluctance principle can be applied to mass transfer into and out of the diffusion layer at electrode/electrolyte interfaces. The results show that the magnetic concentration gradient force arises as a consequence of the minimum reluctance principle. Applied to the diffusion system, the magnetic concentration gradient force arises in the membrane as a consequence of the concentration gradient and hence, the reluctance gradient. The force acts on the flow in such a way that the reluctance in the membrane is minimized. The force implies two interaction mechanisms: attraction of the paramagnetic electrolyte flowing into the membrane in order to decrease the reluctance, and hindrance of the paramagnetic electrolyte flowing out of the membrane in order to hinder an increase in the reluctance. Based on previous studies, it is shown that the minimum reluctance principle can be applied to mass transfer into or out of the diffusion layer at electrode/electrolyte interfaces as well.
Thermal Conductivity of Ionic Liquids and IoNanofluids. Can Molecular Theory Help?
Authors: Paredes Xavier , Lourenço Maria José , Castro Carlos Nieto de , Wakeham William
Published: March 2021

Ionic liquids have been suggested as new engineering fluids, specifically in the area of heat transfer, and as alternatives to current biphenyl and diphenyl oxide, alkylated aromatics and dimethyl polysiloxane oils, which degrade above 200 °C, posing some environmental problems. Addition of nanoparticles to produce stable dispersions/gels of ionic liquids has proved to increase the thermal conductivity of the base ionic liquid, potentially contributing to better efficiency of heat transfer fluids. It is the purpose of this paper to analyze the prediction and estimation of the thermal conductivity of ionic liquids and IoNanofluids as a function of temperature, using the molecular theory of Bridgman and estimation methods previously developed for the base fluid. In addition, we consider methods that emphasize the importance of the interfacial area IL-NM in modelling the thermal conductivity enhancement. Results obtained show that it is not currently possible to predict or estimate the thermal conductivity of ionic liquids with an uncertainty commensurate with the best experimental values. The models of Maxwell and Hamilton are not capable of estimating the thermal conductivity enhancement of IoNanofluids, and it is clear that the Murshed, Leong and Yang model is not practical, if no additional information, either using imaging techniques at nanoscale or molecular dynamics simulations, is available.
Cholesterol Content, Fatty Acid Profile and Health Lipid Indices in the Egg Yolk of Eggs from Hens at the End of the Laying Cycle, Following Alpha-Ketoglutarate Supplementation
Authors: Tomaszewska Ewa , Muszyński Siemowit , Arczewska-Włosek Anna , Domaradzki Piotr , Pyz-Łukasik Renata , Donaldson Janine , Świątkiewicz Sylwester
Published: March 2021

The current study aimed to assess the effects of dietary alpha-ketoglutarate (AKG) supplementation to laying hens on the fatty acid (FA) profile and cholesterol levels of the egg yolk at the end of production cycle. The experiment was performed on forty-eight Bovans Brown laying hens randomly assigned to either a control group (CONT) or a group supplemented with AKG. The CONT group was fed the basal diet, and the AKG group was fed the basal diet plus 1.0% AKG from the 31st until the 60th week of age, when FA profile, fat and cholesterol content of the egg yolks were determined. No significant changes in the cholesterol and total fat content of the egg yolks were observed. However, there were positive (the decrease in n-6 FA and the increase in MUFA), and negative (decrease in PUFA and n-3 FA, increase in TI and n-6/n-3 ratio) changes in FA profile following AKG supplementation. In conclusion, it was shown that dietary AKG after a 30-week long supplementation influence FA profile in egg yolk and its nutritional value.
The Decisive Case-Control Study Elaborates the Null Association between ESR1 XbaI and Osteoarthritis in Asians: A Case–Control Study and Meta-Analysis
Authors: Huang Yu-Hao , Fang Wen-Hui , Tsai Dung-Jang , Chen Yu-Hsuan , Wang Yu-Chiao , Su Wen , Kao Chung-Cheng , Yi Kevin , Wang Chih-Chien , Su Sui-Lung
Published: March 2021

(1) Background: The prevalence of knee osteoarthritis (OA) in women is significantly higher than in men. The estrogen receptor α (ERα) has been considered to play a key role due to a large gender difference in its expression. ERα is encoded by the gene estrogen receptor 1 (ESR1), which is widely studied to explore the gender difference in knee OA. Several polymorphisms in ESR1 [PvuII (rs2234693) and BtgI (rs2228480)] were confirmed as the risk factors of OA. However, the evidence of the last widely investigated polymorphism, ESR1 Xbal (rs9340799), is still insufficient for concluding its effect on knee OA. (2) Objective: This study proposed a case–control study to investigate the association between ESR1 Xbal and knee OA. Moreover, a meta-analysis and trial sequential analysis (TSA) were conducted to enlarge the sample size for obtaining a conclusive evidence. (3) Methods: In total, 497 knee OA cases and 473 healthy controls were recruited between March 2015 and July 2018. The Kellgren–Lawrence grading system was used to identify the knee OA cases. To improve the evidence level of our study, we conducted a meta-analysis including the related studies published up until December 2018 from PubMed, Embase, and previous meta-analysis. The results are expressed as odds ratios (ORs) with corresponding 95% confidence intervals (CI) for evaluating the effect of this polymorphism on knee OA risk. TSA was used to estimate the sample sizes required in this issue. (4) Results: We found non-significant association between the G allele and knee OA [Crude-OR: 0.97 (95% CI: 0.78–1.20) and adjusted-OR: 0.90 (95% CI: 0.71–1.15) in allele model] in the present case–control study, and the analysis of other genetic models showed a similar trend. After including six published studies and our case–control studies, the current evidence with 3174 Asians showed the conclusively null association between ESR1 XbaI and knee OA [OR: 0.78 (95% CI: 0.59–1.04)] with a high heterogeneity (I2: 78%). The result of Caucasians also concluded the null association [OR: 1.05 (95% CI: 0.56–1.95), I2: 87%]. (5) Conclusions: The association between ESR1 XbaI and knee OA was not similar with other polymorphisms in ESR1, which is not a causal relationship. This study integrated all current evidence to elaborate this conclusion for suggesting no necessity of future studies.
MiR-92 Family Members Form a Cluster Required for Notochord Tubulogenesis in Urochordate Ciona savignyi
Authors: Yang Libo , Zhang Xiaoming , Liu Chengzhang , Zhang Jin , Dong Bo
Published: March 2021

MicroRNAs are frequently clustered in the genome and polycistronically transcribed, regulating targeted genes in diverse signaling pathways. The miR-17-92 cluster is a typical miRNA cluster, playing crucial roles in the organogenesis and homeostasis of physiological processes in vertebrates. Here, we identified three miRNAs (csa-miR-92a, csa-miR-92b, and csa-miR-92c) that belonged to the miR-92 family and formed a miRNA cluster in the genome of a urochordate marine ascidian Ciona savignyi. Except for miR-92a and miR-92b, other homologs of the vertebrate miR-17-92 cluster members could not be identified in the Ciona genome. We further found that the mature sequences of urochordate miR-92 family members were highly conserved compared with the vertebrate species. The expression pattern revealed that three miR-92 family members had consistent expression levels in adult tissues and were predominantly expressed in heart and muscle tissue. We further showed that, at the embryonic and larval stages, csa-miR-92c was expressed in the notochord of embryos during 18–31 h post fertilization (hpf) by in situ hybridization. Knockout of csa-miR-92c resulted in the disorganization of notochord cells and the block of lumen coalescence in the notochord. Fibroblast growth factor (FGF), mitogen-activated protein kinase (MAPK), and wingless/integrated (Wnt)/planar cell polarity (PCP) signaling pathways might be involved in the regulatory processes, since a large number of core genes of these pathways were the predicted target genes of the miR-92 family. Taken together, we identified a miR-92 cluster in urochordate Ciona and revealed the expression patterns and the regulatory roles of its members in organogenesis. Our results provide expression and phylogenetic data on the understanding of the miR-92 miRNA cluster’s function during evolution.
Spt4 Promotes Pol I Processivity and Transcription Elongation
Authors: Huffines Abigail K. , Edwards Yvonne J. K. , Schneider David A.
Published: March 2021

RNA polymerases (Pols) I, II, and III collectively synthesize most of the RNA in a eukaryotic cell. Transcription by Pols I, II, and III is regulated by hundreds of trans-acting factors. One such protein, Spt4, has been previously identified as a transcription factor that influences both Pols I and II. Spt4 forms a complex with Spt5, described as the Spt4/5 complex (or DSIF in mammalian cells). This complex has been shown previously to directly interact with Pol I and potentially affect transcription elongation. The previous literature identified defects in transcription by Pol I when SPT4 was deleted, but the necessary tools to characterize the mechanism of this effect were not available at the time. Here, we use a technique called Native Elongating Transcript Sequencing (NET-seq) to probe for the global occupancy of Pol I in wild-type (WT) and spt4△ Saccharomyces cerevisiae (yeast) cells at single nucleotide resolution in vivo. Analysis of NET-seq data reveals that Spt4 promotes Pol I processivity and enhances transcription elongation through regions of the ribosomal DNA that are particularly G-rich. These data suggest that Spt4/5 may directly affect transcription elongation by Pol I in vivo.
Urban Food Autonomy: The Flourishing of an Ethics of Care for Sustainability
Authors: Giraud Esteve
Published: March 2021

Urban agriculture is often advanced as a sustainable solution to feed a growing urban population, offering a number of benefits: improved fresh food access, CO2 absorption, social justice and social cohesion among others. Going beyond these direct tangible/objective benefits from urban agriculture, in this paper we ask: How can growing food in the cities teach us about taking care of each other and the natural environment? We use the example of urban food autonomy movements to discuss the transformative potential of a grassroots-led initiative promoting permaculture, which is anchored in three “ethics”: care for the earth, care for the people, and fair share. Through examining the philosophical underpinnings of “autonomy” and “care”, we explore how urban food autonomy initiatives can enable the development of an ethics of care, especially using permaculture inspirations. Our theoretical review and case analysis reveal that “autonomy” can never be achieved without “care” and that these are co-dependent outcomes. The urban food autonomy initiatives are directly relevant for the achievement of the three of the UN’s 17 Sustainable Development Goals: “Zero Hunger,” “Life on Land” and “Climate Action”, and contribute to a culture of care. Indeed, urban agriculture can act as a powerful education platform for the engagement of diverse stakeholders while also supporting a collective transformation of values.
Surface Shape of the Calcaneal Tuberosity and the Occurrence of Retrocalcaneal Bursitis among Runners
Authors: Wnuk-Scardaccione Agnieszka , Mizia Ewa , Zawojska Klaudia , Bilski Jan , Wojdyła Jakub
Published: March 2021

Purpose: The aim of the study was to establish the relationship between the shape of the calcaneal tuberosity (flat, stepped, rounded, normal) and the probability that retrocalcaneal bursitis among people who train running regularly. Methods: The study included a group of 30 runners who suffered from retrocalcaneal bursitis in the past, and 30 people who never had symptoms of this disease. The study was based on a diagnostic survey, as well as on clinical examination. The surface of the calcaneal tuberosity and the slope of the calcaneus were assessed using X-rays. The mobility of the bursa, its surface size, the thickness of the Achilles tendon and its attachment rate were established during an ultrasound examination. Results: Flat surface of the calcaneal tuberosity increases fourfold the risk of suffering from retrocalcaneal bursitis (OR = 4.3). The people whose calcaneus slope is above 25° are at increased risk of suffering from such an inflammation compared with the people whose calcaneus bone is more horizontal (OR = 2.8). The analysis shows that the thickness of the Achilles tendon (p = 0.001), the surface size of the bursa (p = 0.009), as well as the flat surface of the calcaneal tuberosity (p = 0.008) are strongly associated with the occurrence of retrocalcaneal bursitis. Conclusions: The flat shape of the calcaneal tuberosity increases the risk of bursitis. The risk of inflammation is higher when the Achilles tendon is thicker and the surface of the bursa is smaller than normal.
A Model for the Implementation of Lean Improvements in Healthcare Environments as Applied in a Primary Care Center
Authors: Morell-Santandreu Oscar , Santandreu-Mascarell Cristina , Garcia-Sabater Julio J.
Published: March 2021

Companies operate in a competitive and changing environment requiring increasingly effective and efficient management strategies. Lean is a proven philosophy in the industrial sector having helped companies to adapt to rapid market changes; to economic, technical, and social complexities; and to customer needs. For this reason, companies in the service sector are adopting Lean to improve their service management and to achieve economic, social, and environmental sustainability. This paper presents a model which uses Lean tools to facilitate the introduction of Lean in the management of primary care centers. The results show the implementation of Lean improved primary care center management, achieved stated objectives, and demonstrated faster adaptation to environmental needs and changes. The Lean philosophy developed and applied in the primary care center proved useful at a professional level facilitating developmental changes and prompting lasting improvements by developing a sustainable work culture.
Social Media Use and Body Image Disorders: Association between Frequency of Comparing One’s Own Physical Appearance to That of People Being Followed on Social Media and Body Dissatisfaction and Drive for Thinness
Authors: Jiotsa Barbara , Naccache Benjamin , Duval Mélanie , Rocher Bruno , Grall-Bronnec Marie
Published: March 2021

(1) Summary: Many studies have evaluated the association between traditional media exposure and the presence of body dissatisfaction and body image disorders. The last decade has borne witness to the rise of social media, predominantly used by teenagers and young adults. This study’s main objective was to investigate the association between how often one compares their physical appearance to that of the people they follow on social media, and one’s body dissatisfaction and drive for thinness. (2) Method: A sample composed of 1331 subjects aged 15 to 35 (mean age = 24.2), including 1138 subjects recruited from the general population and 193 patients suffering from eating disorders, completed an online questionnaire assessing social media use (followed accounts, selfies posted, image comparison frequency). This questionnaire incorporated two items originating from the Eating Disorder Inventory Scale (Body Dissatisfaction: EDI-BD and Drive for Thinness: EDI-DT). (3) Results: We found an association between the frequency of comparing one’s own physical appearance to that of people followed on social media and body dissatisfaction and drive for thinness. Interestingly, the level of education was a confounding factor in this relationship, while BMI was not. (4) Discussion: The widespread use of social media in teenagers and young adults could increase body dissatisfaction as well as their drive for thinness, therefore rendering them more vulnerable to eating disorders. We should consequently take this social evolution into account, including it in general population prevention programs and in patients’ specific treatment plans.
General Practice in the Time of COVID-19: A Mixed-Methods Service Evaluation of a Primary Care COVID-19 Service
Authors: Hibberd James , Carter Jessica , McCoy Michaella , Rafiq Meena , Varma Amita , Sanghera Rita , Matthews Philippa , Rait Greta
Published: March 2021

Primary care coronavirus disease 2019 (COVID-19) clinics were rapidly introduced across the UK to review potentially infectious patients. Evaluation of these services is needed to guide future implementation. This mixed-methods study evaluates patient demographics, clinical presentation, co-morbidities, service usage, and outcomes for the Islington COVID-19 service (London, UK) and from April to May 2020 and thematically analyses survey responses from 29 service clinicians and 41 GP referrers on their service experience. Of the 237 patients booked into the service, a significant number of referrals (n = 91; 38.6%) were made after the presumed infectious period of 14 days. Almost half of all adult referrals (49%) were dealt with remotely (via telephone/video consultation +/− remote oxygen saturation monitoring). The service was perceived to provide a safe way to see patients; it developed local expertise, learning, and empowerment; and it was a positive teamworking experience. These findings suggest that the management of many patients with COVID-19 symptoms is possible in routine general practice with minimal risk through the implementation of remote consultation methods and in patients who present after the post-infectious period. Additionally, the use of remote saturation monitoring and local GP COVID-19 “experts” can support practices to manage COVID-19 patients. Future primary care COVID-19 services should act as empowerment tools to assist GPs to safely manage their own patients and provide support for GPs in this process.
Sleep Debt and Social Jetlag Associated with Sleepiness, Mood, and Work Performance among Workers in Japan
Authors: Okajima Isa , Komada Yoko , Ito Wakako , Inoue Yuichi
Published: March 2021

Although sleep debt and social jetlag (SJL) influence daytime dysfunctions, the effects of both sleep debt and SJL on them have not been analyzed. The aim of this study was to examine the mutual relationship between sleep debt and SJL on daytime sleepiness, mood, and work performance. This study was a cross-sectional study on sleep health conducted on the Japanese general population. A total of 4505 general workers (30% female, aged 43.57 ± 11.63 years) were selected and analyzed. Sleep debt was defined by sleep debt index (SDI), which is the discrepancy between desired and real sleep duration. SJL and SDI scores exhibited a positive but weak coefficient (r = 0.19). In a 4 (SJL) × 3 (SDI) two-way ANOVA, the interaction effects were notable for sleepiness and depression scores, while the group effects were notable for the work performance score. For sleepiness and depression scores, SDI >2 h was not significantly different from SJL. In addition, the impact of SDI was higher than that of SJL on sleepiness (β = 0.17), depression (β = 0.16), and work performance (β = −0.10). The impact of sleep debt was more pronounced than SJL on daytime dysfunctions, although both sleep debt and SJL have negative impacts on them.
COVID-19 Impact on SDGs and the Fiscal Measures: Case of Indonesia
Authors: Safitri Yulida , Ningsih Reni Diah , Agustianingsih Dwi Putri , Sukhwani Vibhas , Kato Akiko , Shaw Rajib
Published: March 2021

The implications of the ongoing COVID-19 pandemic have stretched far beyond human health and wellbeing, causing serious setbacks for the achievement of the Sustainable Development Goals (SDGs). Although governments worldwide have implemented different fiscal stimulus measures to mitigate the implications of COVID-19, it is important to develop a precise understanding of their focus areas to ensure if the progress of SDGs is on track. For a specific case of Indonesia, this study establishes a thorough understanding of the COVID-19 implications on SDGs, and its fiscal stimulus package through a literature review and semi-formal interviews with the core stakeholders in Indonesia. The study results highlighted that COVID-19 has varyingly affected the progress of all SDGs in Indonesia. Amongst the four pillars of sustainable development in Indonesia, the SDGs on the social and economic development pillars are stated to be the most impacted. As for the fiscal stimulus, it is perceived that it can help maintain the SDGs’ attainment progress to a certain extent, although there are several concerns on its implementation. Deriving lessons from the conducted research, the study puts forward key suggestions for the effective implementation of SDGs in the post-COVID-19 era.
Knowledge and Practices of Community Pharmacists in Topical Dermatological Treatments
Authors: Teixeira Ana , Teixeira Maribel , Herdeiro Maria Teresa , Vasconcelos Viviana , Correia Rita , Bahia Maria Fernanda , Almeida Isabel F. , Vidal Diogo Guedes , Sousa Hélder Fernando Pedrosa e , Dinis Maria Alzira Pimenta , Almeida Vera
Published: March 2021

The connection between pharmacists’ knowledge and practice on the provided information to patients about dermatoses and their treatment is insufficiently characterized. Furthermore, pharmacists’ contributions in counselling and in promoting adherence to topical treatment is not fully understood. This study has three main objectives. It aims to identify the knowledge and practices of pharmacists about dermatoses and their treatment, and to compare the perspective of pharmacists with that of patients regarding treatment information, with the future goal of establishing guidelines on the communication of dosage regimen instructions to dermatological patients and promotion of adherence to treatment, filling a gap. A cross-sectional, exploratory, and descriptive study was carried out. Based on experts’ prior knowledge and extensive collected literature information, two questionnaire protocols, one for pharmacists and another one for patients, were designed. Exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) were carried out in relation to the pharmacists’ questionnaire for instrument validation. The results indicate that knowledge of pharmacists regarding dermatoses and their treatment is considered acceptable. Most of the pharmacists were reported to provide information to patients. Oppositely, patients reported not to have receive it. This is an important issue because pharmacists play a primary role in the management of several diseases. As non-adherence can be triggered by poor understanding of the dosing instructions, pharmacists’ communication practices play an important role in improving this hinderance. Results from this study identified pharmacist–patient communication gaps, so the development of guidelines to improve the transmission of clear dosage regimen instructions and knowledge about patient’s disease are of paramount importance. Training programs for continuous education of pharmacist should be implemented to solve the identified communication problems found in this study.
Seroepidemiologic Survey of Lyme Disease among Forestry Workers in National Park Offices in South Korea
Authors: Acharya Dilaram , Park Ji-Hyuk
Published: March 2021

Limited data are available on the current status of Lyme disease in South Korea. The aim of this study was to investigate the seroprevalence and risk factors associated with Lyme disease infection among forestry workers in National Park Offices in South Korea. We enrolled National Park Office forestry workers (NPOFWs) who had worked for ≥1 year. Participants completed questionnaires that addressed various subjects including work types and work hygiene-related factors. Collected serum samples were tested using immunofluorescence assay to detect anti-Borrelia antibodies. Multivariate logistic regression was used to identify independent risk factors of seroprevalence. Of 1,410 NPOFWs, 655 (46.5%) participated in this study, and an overall seroprevalence of Lyme disease antibodies was 8.1%. Analysis showed that always eating meals in woodland (odds ratio (OR), 5.11; 95% confidence interval (CI), 2.08–12.52) and raising dogs outside homes (OR, 3.25; 95% CI, 1.57–6.75) were significantly associated with Lyme disease infection. This seroprevalence study indicates that Lyme disease is an important disease among NPOFWs in South Korea. These identified modifiable risk factors should be considered while designing preventive strategies for reducing Lyme disease infection among NPOFWs.
Cerebral Expression of Metabotropic Glutamate Receptor Subtype 5 in Idiopathic Autism Spectrum Disorder and Fragile X Syndrome: A Pilot Study
Authors: Brašić James Robert , Nandi Ayon , Russell David S. , Jennings Danna , Barret Olivier , Martin Samuel D. , Slifer Keith , Sedlak Thomas , Seibyl John P. , Wong Dean F. , Budimirovic Dejan B.
Published: March 2021

Multiple lines of evidence suggest that dysfunction of the metabotropic glutamate receptor subtype 5 (mGluR5) plays a role in the pathogenesis of autism spectrum disorder (ASD). Yet animal and human investigations of mGluR5 expression provide conflicting findings about the nature of dysregulation of cerebral mGluR5 pathways in subtypes of ASD. The demonstration of reduced mGluR5 expression throughout the living brains of men with fragile X syndrome (FXS), the most common known single-gene cause of ASD, provides a clue to examine mGluR5 expression in ASD. We aimed to (A) compare and contrast mGluR5 expression in idiopathic autism spectrum disorder (IASD), FXS, and typical development (TD) and (B) show the value of positron emission tomography (PET) for the application of precision medicine for the diagnosis and treatment of individuals with IASD, FXS, and related conditions. Two teams of investigators independently administered 3-[18F]fluoro-5-(2-pyridinylethynyl)benzonitrile ([18F]FPEB), a novel, specific mGluR5 PET ligand to quantitatively measure the density and the distribution of mGluR5s in the brain regions, to participants of both sexes with IASD and TD and men with FXS. In contrast to participants with TD, mGluR5 expression was significantly increased in the cortical regions of participants with IASD and significantly reduced in all regions of men with FXS. These results suggest the feasibility of this protocol as a valuable tool to measure mGluR5 expression in clinical trials of individuals with IASD and FXS and related conditions.
A Scoping Review on Lipocalin-2 and Its Role in Non-Alcoholic Steatohepatitis and Hepatocellular Carcinoma
Authors: Krizanac Marinela , Mass Sanchez Paola Berenice , Weiskirchen Ralf , Asimakopoulos Anastasia
Published: March 2021

Excess calorie intake and a sedentary lifestyle have made non-alcoholic fatty liver disease (NAFLD) one of the fastest growing forms of liver disease of the modern world. It is characterized by abnormal accumulation of fat in the liver and can range from simple steatosis and non-alcoholic steatohepatitis (NASH) to cirrhosis as well as development of hepatocellular carcinoma (HCC). Biopsy is the golden standard for the diagnosis and differentiation of all NAFLD stages, but its invasiveness poses a risk for patients, which is why new, non-invasive ways of diagnostics ought to be discovered. Lipocalin-2 (LCN2), which is a part of the lipocalin transport protein family, is a protein formally known for its role in iron transport and in inflammatory response. However, in recent years, its implication in the pathogenesis of NAFLD has become apparent. LCN2 shows significant upregulation in several benign and malignant liver diseases, making it a good candidate for the NAFLD biomarker or even a therapeutic target. What makes LCN2 more interesting to study is the fact that it is overexpressed in HCC development induced by chronic NASH, which is one of the primary causes of cancer-related deaths. However, to this day, neither its role as a biomarker for NAFLD nor the molecular mechanisms of its implication in NAFLD pathogenesis have been completely elucidated. This review aims to gather and closely dissect the current knowledge about, sometimes conflicting, evidence on LCN2 as a biomarker for NAFLD, its involvement in NAFLD, and NAFLD-HCC related pathogenesis, while comparing it to the findings in similar pathologies.
Effects of Cannabidiol and Beta-Caryophyllene Alone or in Combination in a Mouse Model of Permanent Ischemia
Authors: Yokubaitis Cody G. , Jessani Hassan N. , Li Hongbo , Amodea Allison K. , Ward Sara Jane
Published: March 2021

Current treatments for stroke, which account for 6.5 million global deaths annually, remain insufficient for treatment of disability and mortality. One targetable hallmark of stroke is the inflammatory response following infarct, which leads to significant damage post-infarct. Cannabinoids and their endogenous targets within the CNS have emerged as potential treatments for neuroinflammatory indications. We and others have previously shown that synthetic agonists of the cannabinoid CB2 receptor reduce infarct size and microglial activation in rodent models of stroke. The non-cannabinoid receptor mediated effects of the phytocannabinoid cannabidiol (CBD) have also shown effectiveness in these models. The present aim was to determine the single and combined effects of the cannabis-derived sesquiterpene and putative CB2 receptor agonist β-caryophyllene (BCP) and CBD on permanent ischemia without reperfusion using a mouse model of photothrombosis. Because BCP and CBD likely work through different sites of action but share common mechanisms of action, we sought to determine whether combinations of BCP and CBD were more potent than either compound alone. Therefore we determined the effect of BCP (3–30 mg/kg IP) and CBD (3–30 mg/kg IP), given alone or in combination (30:3, 30:10, and 30:30 BCP:CBD), on infarct size, microglial activation, and motor performance.
Translocator Protein Modulation by 4′-Chlorodiazepam and NO Synthase Inhibition Affect Cardiac Oxidative Stress, Cardiometabolic and Inflammatory Markers in Isoprenaline-Induced Rat Myocardial Infarction
Authors: Ilic Ana , Todorovic Dusan , Mutavdzin Slavica , Boricic Novica , Bozic Nedeljkovic Biljana , Stankovic Sanja , Simic Tatjana , Stevanovic Predrag , Celic Vera , Djuric Dragan
Published: March 2021

The possible cardioprotective effects of translocator protein (TSPO) modulation with its ligand 4′-Chlorodiazepam (4′-ClDzp) in isoprenaline (ISO)-induced rat myocardial infarction (MI) were evaluated, alone or in the presence of L-NAME. Wistar albino male rats (b.w. 200–250 g, age 6–8 weeks) were divided into 4 groups (10 per group, total number N = 40), and certain substances were applied: 1. ISO 85 mg/kg b.w. (twice), 2. ISO 85 mg/kg b.w. (twice) + L-NAME 50 mg/kg b.w., 3. ISO 85 mg/kg b.w. (twice) + 4′-ClDzp 0.5 mg/kg b.w., 4. ISO 85 mg/kg b.w. (twice) + 4′-ClDzp 0.5 mg/kg b.w. + L-NAME 50 mg/kg b.w. Blood and cardiac tissue were sampled for myocardial injury and other biochemical markers, cardiac oxidative stress, and for histopathological evaluation. The reduction of serum levels of high-sensitive cardiac troponin T hs cTnT and tumor necrosis factor alpha (TNF-α), then significantly decreased levels of serum homocysteine Hcy, urea, and creatinine, and decreased levels of myocardial injury enzymes activities superoxide dismutase (SOD) and glutathione peroxidase (GPx) as well as lower grades of cardiac ischemic changes were demonstrated in ISO-induced MI treated with 4′-ClDzp. It has been detected that co-treatment with 4′-ClDzp + L-NAME changed the number of registered parameters in comparison to 4′-ClDzp group, indicating that NO (nitric oxide) should be important in the effects of 4′-ClDzp.
The Participation of the Intrinsically Disordered Regions of the bHLH-PAS Transcription Factors in Disease Development
Authors: Kolonko-Adamska Marta , Uversky Vladimir N. , Greb-Markiewicz Beata
Published: March 2021

The basic helix–loop–helix/Per-ARNT-SIM (bHLH-PAS) proteins are a family of transcription factors regulating expression of a wide range of genes involved in different functions, ranging from differentiation and development control by oxygen and toxins sensing to circadian clock setting. In addition to the well-preserved DNA-binding bHLH and PAS domains, bHLH-PAS proteins contain long intrinsically disordered C-terminal regions, responsible for regulation of their activity. Our aim was to analyze the potential connection between disordered regions of the bHLH-PAS transcription factors, post-transcriptional modifications and liquid-liquid phase separation, in the context of disease-associated missense mutations. Highly flexible disordered regions, enriched in short motives which are more ordered, are responsible for a wide spectrum of interactions with transcriptional co-regulators. Based on our in silico analysis and taking into account the fact that the functions of transcription factors can be modulated by posttranslational modifications and spontaneous phase separation, we assume that the locations of missense mutations inducing disease states are clearly related to sequences directly undergoing these processes or to sequences responsible for their regulation.
Recent Advances in Iron Chelation and Gallium-Based Therapies for Antibiotic Resistant Bacterial Infections
Authors: Vinuesa Víctor , McConnell Michael J.
Published: March 2021

Iron is essential for multiple bacterial processes and is thus required for host colonization and infection. The antimicrobial activity of multiple iron chelators and gallium-based therapies against different bacterial species has been characterized in preclinical studies. In this review, we provide a synthesis of studies characterizing the antimicrobial activity of the major classes of iron chelators (hydroxamates, aminocarboxylates and hydroxypyridinones) and gallium compounds. Special emphasis is placed on recent in-vitro and in-vivo studies with the novel iron chelator DIBI. Limitations associated with iron chelation and gallium-based therapies are presented, with emphasis on limitations of preclinical models, lack of understanding regarding mechanisms of action, and potential host toxicity. Collectively, these studies demonstrate potential for iron chelators and gallium to be used as antimicrobial agents, particularly in combination with existing antibiotics. Additional studies are needed in order to characterize the activity of these compounds under physiologic conditions and address potential limitations associated with their clinical use as antimicrobial agents.
Immune Stroma in Lung Cancer and Idiopathic Pulmonary Fibrosis: A Common Biologic Landscape?
Authors: Lettieri Sara , Oggionni Tiberio , Lancia Andrea , Bortolotto Chandra , Stella Giulia Maria
Published: March 2021

Idiopathic pulmonary fibrosis (IPF) identifies a specific entity characterized by chronic, progressive fibrosing interstitial pneumonia of unknown cause, still lacking effective therapies. Growing evidence suggests that the biologic processes occurring in IPF recall those which orchestrate cancer onset and progression and these findings have already been exploited for therapeutic purposes. Notably, the incidence of lung cancer in patients already affected by IPF is significantly higher than expected. Recent advances in the knowledge of the cancer immune microenvironment have allowed a paradigm shift in cancer therapy. From this perspective, recent experimental reports suggest a rationale for immune checkpoint inhibition in IPF. Here, we recapitulate the most recent knowledge on lung cancer immune stroma and how it can be translated into the IPF context, with both diagnostic and therapeutic implications.
Strategies to Modulate Specialized Metabolism in Mediterranean Crops: From Molecular Aspects to Field
Authors: Balestrini Raffaella , Brunetti Cecilia , Cammareri Maria , Caretto Sofia , Cavallaro Valeria , Cominelli Eleonora , De Palma Monica , Docimo Teresa , Giovinazzo Giovanna , Grandillo Silvana , Locatelli Franca , Lumini Erica , Paolo Dario , Patanè Cristina , Sparvoli Francesca , Tucci Marina , Zampieri Elisa
Published: March 2021

Plant specialized metabolites (SMs) play an important role in the interaction with the environment and are part of the plant defense response. These natural products are volatile, semi-volatile and non-volatile compounds produced from common building blocks deriving from primary metabolic pathways and rapidly evolved to allow a better adaptation of plants to environmental cues. Specialized metabolites include terpenes, flavonoids, alkaloids, glucosinolates, tannins, resins, etc. that can be used as phytochemicals, food additives, flavoring agents and pharmaceutical compounds. This review will be focused on Mediterranean crop plants as a source of SMs, with a special attention on the strategies that can be used to modulate their production, including abiotic stresses, interaction with beneficial soil microorganisms and novel genetic approaches.