bluuebunny/crossref_metadata_2025 huggingface dataset:
Povećanje učinkovitosti bioremedijacije na razini gena
Authors: Česnik Morana , Findrik Blažević Zvjezdana , Vuković Domanovac Marija
Published: January 2019

Bioremedijacija se koristi potencijalom mikroorganizama pri uklanjanju onečišćenja iz okoliša. Metabolički putovi kojima se onečišćujuća tvar razgrađuje u manje toksične tvari vrlo su kompleksni. Naprednim tehnikama molekularne biologije mehanizmi bioremedijacije izučavaju se na razini gena. Gen zaslužan za proizvodnju proteina koji razgrađuje onečišćujuću tvar može se izolirati i unijeti u drugi organizam, čime nastaje organizam s poboljšanim bioremedijacijskim svojstvima. Brojna se svjetska istraživanja temelje na genetičkom inženjerstvu na mikroorganizmima, no najveću prepreku upotrebe takvih organizama u bioremedijaciji čine zakonski okviri te nedovoljno poznavanje posljedica njihova oslobađanja u okoliš.
Next generation sequencing (NGS) to improve the diagnosis and management of patients with disorders of sex development (DSD)
Authors: Hughes L A , McKay-Bounford K , Webb E A , Dasani P , Clokie S , Chandran H , McCarthy L , Mohamed Z , Kirk J M W , Krone N P , Allen S , Cole T R P
Published: January 2019

Disorders of sex development (DSDs) are a diverse group of conditions where the chromosomal, gonadal or anatomical sex can be atypical. The highly heterogeneous nature of this group of conditions often makes determining a genetic diagnosis challenging. Prior to next generation sequencing (NGS) technologies, genetic diagnostic tests were only available for a few of the many DSD-associated genes, which consequently had to be tested sequentially. Genetic testing is key in establishing the diagnosis, allowing for personalised management of these patients. Pinpointing the molecular cause of a patient’s DSD can significantly impact patient management by informing future development needs, altering management strategies and identifying correct inheritance pattern when counselling family members. We have developed a 30-gene NGS panel, designed to be used as a frontline test for all suspected cases of DSD (both 46,XX and 46,XY cases). We have confirmed a diagnosis in 25 of the 80 patients tested to date. Confirmed diagnoses were linked to mutations inAMH,AMHR2,AR,HSD17B3,HSD3B2,MAMLD1,NR5A1,SRD5A2andWT1which have resulted in changes to patient management. The minimum diagnostic yield for patients with 46,XY DSD is 25/73. In 34/80 patients, only benign or likely benign variants were identified, and in 21/80 patients only variants of uncertain significance (VOUS) were identified, resulting in a diagnosis not being confirmed in these individuals. Our data support previous studies that an NGS panel approach is a clinically useful and cost-effective frontline test for patients with DSDs.
Targetable gene fusions identified in radioactive iodine refractory advanced thyroid carcinoma
Authors: van der Tuin K , Ventayol Garcia M , Corver W E , Khalifa M N , Ruano Neto D , Corssmit E P M , Hes F J , Links T P , Smit J W A , Plantinga T S , Kapiteijn E , van Wezel T , Morreau H
Published: January 2019

Objective

Gene alterations leading to activation of the MAPK pathway are of interest for targeted therapy in patients with advanced radioactive iodine refractory (RAI-R) thyroid carcinoma. Due to technical reasons gene fusion analysis in RNA isolated from formalin-fixed tumor tissues has till now been limited. The objective of the present study was to identify targetable gene rearrangements in RNA isolated from formalin-fixed RAI-R thyroid carcinomas.

Design

Retrospective study in 132 patients with RAI-R thyroid carcinoma (59 papillary-, 24 follicular-, 35 Hürthle cell- and 14 anaplastic thyroid carcinoma).

Methods

Total nucleic acid (undivided DNA and RNA) was isolated from formalin-fixed tissue. Extensive gene fusion analysis was performed in all samples that tested negative for pathogenicBRAF,NRAS,HRASandKRASvariants.

Results

Seven targetable gene fusions were identified in the remaining 60 samples without known DNA variants. This includes frequently reported gene fusions such asCCDC6/RET(PTC1),PRKAR1A/RET(PTC2) andETV6/NTRK3, and gene fusions that are less common in thyroid cancer (TPM3/NTRK1,EML4/ALKandEML4/NTRK3). Of note, most gene fusions were detected in papillary thyroid carcinoma and MAPK-associated alterations in Hürthle cell carcinomas are rare (2/35).

Conclusion

Targetable gene fusions were found in 12% of RAI-R thyroid carcinoma without DNA variants and can be effectively identified in formalin-fixed tissue. These gene fusions might provide a preclinical rationale to include specific kinase inhibitors in the treatment regimen for these patients. The latter intends to restore iodine transport and/or take advantage of the direct effect on tumor cell vitality once progressive disease is seen.
Risk of all-cause and CHD mortality in women versus men with type 2 diabetes: a systematic review and meta-analysis
Authors: Xu Guodong , You Dingyun , Wong Liping , Duan Donghui , Kong Fanqian , Zhang Xiaohong , Zhao Jinshun , Xing Wenhua , Han Liyuan , Li Li
Published: January 2019

Objective

Previous studies have shown sex-specific differences in all-cause and CHD mortality in type 2 diabetes. We performed a systematic review and meta-analysis to provide a global picture of the estimated influence of type 2 diabetes on the risk of all-cause and CHD mortality in women vs men.

Methods

We systematically searched PubMed, EMBASE and Web of Science for studies published from their starting dates to Aug 7, 2018. The sex-specific hazard ratios (HRs) and their pooled ratio (women vs men) of all-cause and CHD mortality associated with type 2 diabetes were obtained through an inverse variance-weighted random-effects meta-analysis. Subgroup analyses were used to explore the potential sources of heterogeneity.

Results

The 35 analyzed prospective cohort studies included 2 314 292 individuals, among whom 254 038 all-cause deaths occurred. The pooled women vs men ratio of the HRs for all-cause and CHD mortality were 1.17 (95% CI: 1.12–1.23, I2 = 81.6%) and 1.97 (95% CI: 1.49–2.61, I2 = 86.4%), respectively. The pooled estimate of the HR for all-cause mortality was approximately 1.30 in articles in which the duration of follow-up was longer than 10 years and 1.10 in articles in which the duration of follow-up was less than 10 years. The pooled HRs for all-cause mortality in patients with type 2 diabetes was 2.33 (95% CI: 2.02–2.69) in women and 1.91 (95% CI: 1.72–2.12) in men, compared with their healthy counterparts.

Conclusions

The effect of diabetes on all-cause and CHD mortality is approximately 17 and 97% greater, respectively, for women than for men.
Ejaculate-mediated paternal effects: evidence, mechanisms and evolutionary implications
Authors: Evans Jonathan P , Wilson Alastair J , Pilastro Andrea , Garcia-Gonzalez Francisco
Published: January 2019

Despite serving the primary objective of ensuring that at least one sperm cell reaches and fertilises an ovum, the male ejaculate (i.e. spermatozoa and seminal fluid) is a compositionally complex ‘trait’ that can respond phenotypically to subtle changes in conditions. In particular, recent research has shown that environmentally and genetically induced changes to ejaculates can have implications for offspring traits that are independent of the DNA sequence encoded into the sperm’s haploid genome. In this review, we compile evidence from several disciplines and numerous taxonomic systems to reveal the extent of such ejaculate-mediated paternal effects (EMPEs). We consider a number of environmental and genetic factors that have been shown to impact offspring phenotypes via ejaculates, and where possible, we highlight the putative mechanistic pathways by which ejaculates can act as conduits for paternal effects. We also highlight how females themselves can influence EMPEs, and in some cases, how maternally derived sources of variance may confound attempts to test for EMPEs. Finally, we consider a range of putative evolutionary implications of EMPEs and suggest a number of potentially useful approaches for exploring these further. Overall, our review confirms that EMPEs are both widespread and varied in their effects, although studies reporting their evolutionary effects are still in their infancy.
Evidence that growth hormone can improve mitochondrial function in oocytes from aged mice
Authors: Hou Hai-Yan , Wang Xi , Yu Qi , Li Hong-Yi , Li Shao-Jie , Tang Rui-Yi , Guo Zai-Xin , Chen Ya-Qiong , Hu Chun-Xiu , Yang Zhi-Juan , Zhang Wen-ke , Qin Yan
Published: January 2019

Decline in successful conception decreases more rapidly after 38 years of age owing to follicular depletion and decreased oocyte quality. However, limited information is available regarding the underlying mechanism and the useful treatment. This study aimed to evaluate the effects of growth hormone supplementation on oocyte maturationin vivoin aged and young mice and to determine its effect on mitochondrial function. The influence of three different doses of recombinant human growth hormone (rhGH) (0.4, 0.8 and 1.6 mg/kg/day) for 8 weeks before ovarian stimulation was analyzed. Superovulated oocytes were released from the oviduct of 12-week-old and 40-week-old female C57BL/6J mice 14–16 h after administration of human chorionic gonadotropin. Ovarian follicle and morphological analysis and oocyte maturation parameters were then evaluated. This study is the first, to our knowledge, to report that medium- and high-dose rhGH significantly increases antral follicles in aged mice but anti-Müllerian hormone (AMH) levels. Furthermore, derived oocytes, MII-stage oocyte rate, ATP levels, mitochondrial membrane potential and frequencies of homogeneous mitochondrial distribution increased. In contrast, in both aged and young mice, the mtDNA copy numbers per oocyte were similar before rhGH administration, and upon saline administration, they did not differ significantly. We conclude that medium-dose rhGH supplementation before standard ovarian stimulation regimens improves oocyte quality in aged mice, probably by enhancing mitochondrial functionality.
Post-tyrimas ugdymo moksluose: ontologinės ir epistemologinės įžvalgos
Authors: Garbauskaitė-Jakimovska Justina
Published: January 2019

[straipsnis ir santrauka lietuvių kalba; santrauka anglų kalba]

Šiuo straipsniu siekiama plėsti mokslinį diskursą post tyrimų metodologijos tema. Straipsnyje apžvelgiamos pagrindinės postmodernizmo, poststruktūralizmo ir posthumanizmo idėjos ir jų perkėlimas į mokslinius tyrimus ugdymo srityje, kintantis požiūris į duomenis, filosofijos svarbą, tyrimų atlikimo procedūras ir duomenų pristatymą. Pagrindinės idėjos iliustruojamos užsienio mokslininkų atliktų tyrimų pavyzdžiais.
Apie Simonos Kontrimienės daktaro disertaciją „humanistinio dvasingumo ryšys su auklėjimo šeimoje patirtimis“
Authors: Targamadzė Vilija
Published: January 2019

[tekstas lietuvių kalba]
Krūtinkaulio pseudoartrozės po resternotomijos gydymas Strasbūro krūtinės osteosintezės sistema: klinikinis atvejis
Authors: Janilionis Ričardas , Falcoz Pierre-Emmanuel , Jovaišas Vytautas , Jagelavičius Žymantas , Valančius Ramūnas
Published: January 2019

Sternal pseudoarthrosis after resternotomy treated with the Strasbourg Thoracic Osteosyntheses System: a case report
Sternal dehiscence is a serious complication after general thoracic and cardiac surgery. Sternal resuturing, performed by simple rewiring or technical modification of rewiring, can fail overall when the bone quality is poor or the sternum is completely destroyed. A number of different sternal closure systems consisting of plates, screws, clips, and titanium bars have been recently introduced to treat the complicated sternal dehiscence. We describe the use of the Strasbourg Thoracic Osteosyntheses System (STRATOS) to treat complicated sternal dehiscence causing chest and back pain, sternum and chest instability, which was applied for the first time in Lithuania.Key words: sternotomy, sternal pseudoarthrosis, sternal osteosynthesis, chest instability, STRATOS.
Recidyvų vertinimas po hallux valgus korekcijos Lapidus operacijos ir Z-osteotomijos metodais
Authors: Janavičius Julius , Kocius Manvilius
Published: January 2019

Tirti pacientai, dėl hallux valgus patologijos operuoti Respublikinėje Vilniaus universitetinėje ligoninėje 2006–2008 metais, atliekant Z-osteotomiją arba Lapidus operaciją: 70 pacientų (4 vyrai, 66 moterys), kuriems buvo operuota 80 pėdų (4 – vyrų, 76 – moterų) – po 40 atvejų abiejų operacijos tipų. Tirtų pacientų priešoperaciniai patologijos kriterijai buvo identiški ir sutapo svarbiausias indikacijų parametras – kampas tarp pirmo ir antro padikaulių (IMT1) buvo 16°–23°. Darbo tikslas – palyginti šių grupių recidyvų skaičių. Palyginus grupių pooperacinius rezultatus paaiškėjo, kad recidyvų skaičius vertinant tiek HV kampą, tiek IMT1 kampą po Z-osteotomijos yra mažesnis. Kadangi Z-osteotomija yra mažesnės apimties operacija ir techniškai paprastesnė, o pooperacinis laikotarpis trumpesnis, esant IMT1 kampui 16°–23°, ši operacija turėtų būti pasirenkama pirmiausiai. MTC1 sąnario artrodezė neapsaugo nuo hallux valgus deformacijos.Assessment of relapse after halux valgus Lapidus correction surgery and Z-osteotomy techniques
The aim of this study was to compare the number of relapses in two analogous groups after hallux valgus correction surgery by the Lapidus and Z-osteotomy techniques. The study included 70 patients (4 men, 66 women), who met the inclusion criteria. In total, 80 feet were analyzed (4 males and 76 female), i. e. two comparable groups of 40 feet. The postoperative results showed that the number of relapses as regards both the hallus valgus and the IMT1 angles was less after Z-osteotomy.
Žmogaus chorioninio gonadotropino reikšmė klinikinėje praktikoje
Authors: Tamašauskas Oldas , Šiaudinytė Ieva
Published: January 2019

Žmogaus chorioninio gonadotropino (hCG) koncentracijos nustatymas dažniausiai naudojamas ankstyvo nėštumo diagnostikai, trukmei nustatyti bei nėštumui stebėti. Be to, HCG yra vertingas diagnozuojant savaiminį persileidimą, nesivystantį arektopinį nėštumą. Šis hormonas leidžia prenataliai įvertinti vaisiaus apsigimimų riziką. Taip pat hCG labai svarbus trofoblastinių ligų ir tam tikrų lokalizacijų vėžio diagnostikai, gydymo efektyvumo vertinimui. Retai, tačiau galimi ir klaidingai teigiamityrimų rezultatai dėl analitinių klaidų, tyrimams naudojamų įrenginių, prietaisų gedimų ar tam tikrų kraujyje esančių medžiagų (pvz.: heterofiliniai antikūnai, autoantikūnai, reumatoidinis faktorius, taip pat bilirubinas, hemoglobinas ar lipidai galilemti klaidingus, netikslius tyrimo rezultatus), todėl svarbu įvertinti ne vieno tyrimo rezultatus, o bendrą klinikinį vaizdą, visą surinktą informaciją ir apsvarstyti visas klinikinės diagnozės galimybes.Reikšminiai žodžiai: žmogaus chorioninis gonadotropinas, hCG, ektopinis nėštumas, klaidingai teigiamas rezultatas, Immulite2000, nėštumo diagnostika.The significance of human chorionic gonadotropin in clinical practice
pregnancy, assess its duration and observe its course. In addition, hCG is a valuable tool in the diagnostics of miscarriage, nondeveloping foetus or ectopic pregnancy. The measurements of this hormone allow to suspect the possible risk of foetalabnormalities. hCG is also used to diagnose and follow the course of particular types of cancer and diseases of throphoblastic origin. Rarely, though, it is possible that false positive results ensue due to failures of analysis, malfunction of laboratory equipment or particular substances circulating in the blood, i.e. heterophilic antibodies, autoantibodies, the rheumatoid factor, bilirubin, haemoglobin or lipids could possibly lead to false test results. Taking into consideration all the previously mentionedissues, it is always important to assess the whole clinical status of the patient, to gather all the possible information and to consider various diagnostic approaches and not to rely too heavily on one laboratory test.Key words: human chorionic gonadotropin, ectopic pregnancy, hCG, false-positive rezult, Immulite2000, pregnancy diagnostics
Onkologinių pacientų vaisingumo išsaugojimas: klinikinis atvejis
Authors: Amšiejienė Andrė , Usonienė Audronė , Šiaudinytė Ieva
Published: January 2019

Onkologinių ligų nuolat daugėja, tačiau tobulėjančios gydymo galimybės labai didina išgyvenamumo rodiklius. Tai rodo vis didėjantį onkologinių pacientų gyvenimo kokybės po sėkmingo gydymo temos aktualumą. Prieš skiriant gonadotoksinį gydymą nuo vėžio, svarbu informuoti kiekvieną vaisingo amžiaus pacientę apie riziką pakenkti vaisingumui. Turėtų būti aptartas ir rekomenduotas vaisingumą išsaugantis gydymas. Kiaušidžių transpozicija, kiaušialąsčių ar embrionų kriokonservacija, kiaušidės audinio užšaldymas ir gonadotropinio hormono agonistų terapija – tai klinikiniais tyrimais pagrįsti vaisingumą išsaugantys gydymo metodai. Svarbu parinkti tinkamiausią ir priimtiniausią metodą, atsižvelgiant į moters amžių, kiaušidžių išteklius, socialinę ekonominę padėtį, religines pažiūras, sveikatos būklę ir gretutines ligas.Reikšminiai žodžiai: onkologiniai pacientai, gydymas nuo vėžio, vaisingumo išsaugojimas, kiaušidžių transpozicija, embrionų užšaldymas, kiaušialąsčių užšaldymas, kiaušidės audinio kriokonservacija ir transplantacija, gonadotropinių hormonų agonistaiFertility preservation in patients with cancer: case report
The total number of oncological diseases is constantly growing, but the improvement of treatment options significantly increases the survival rates. It shows the importance of the oncological patients’ quality of life after a succesful treatment. Prior to the prescription of any gonadotoxic medications, every patient of a fertile age should be informed about the risks of damaging fertility and recommended protective treatments. Ovarian transposition, oocyte, embryo or ovarian tissue cryoconservation and gonadotropin releasing hormone agonists’ (GnRHa) therapy these are the clinically tested fertility-protecting treatment methods. For the appropriate method to be selected, it is very important to take into consideration the female’s age, ovarian condition, social-economic situation, religious views, overall health condition, and related diseases.Key words: oncological patients, fertility protection, ovarian transposition, embryo cryoconservation, oocyte cryoconservation, ovarian tissue cryoconservation and transplantation, gonadotropin releasing hormone agonists
Chirurgai ir akademinės chirurgijos raida Kaune 1919–1940 metais
Authors: Zykas Vytautas
Published: January 2019

Straipsnyje apžvelgiama chirurgijos raida nepriklausomoje Lietuvoje 1919–1940 metais. Pateikiami duomenys apie pirmuosius žingsnius organizuojant ir kuriant chirurgijos discipliną Aukštuosiuose kursuose ir vėlesnį jos vystymąsi universiteto Medicinos fakultete Kaune. Aprašoma iškiliausių to laikotarpio chirurgų J. Žemgulio, J. Dzirnės, H. Boito, A. Hagentorno, V. Kanaukos, V. Kuzmos, A. Maciūno, J. Čeponio, J. Jaržemsko ir kt. veikla kuriant praktinę ir akademinę chirurgiją Kaune ir Lietuvoje, aptariamas jų indėlis į mokslą, Lietuvos chirurgų draugijos organizavimą, pirmojo periodinio lietuviško žurnalo „Medicina“ steigimą. Pateikiami svarbesni faktai apie šių iškilių asmenybių visuomeninę veiklą, jų tragiškus likimus istorijos posūkiuose.

Surgeons and academic surgery in Kaunas between 1919–1940Prof. habil. dr. Vytautas Zykas

This article offers an historical overview of the development of surgery in Lithuania during the years of independence between 1919 and 1940. It describes the first steps of the creation of academic surgery and it‘s later development in the Universtity in Kaunas. The paper depicts facts about the prominent surgeons of that period – J. Žemgulys, J. Dzirnė, H. Boit, A. Hagentorn, V. Kanauka, A. Maciūnas, J. Čeponis, J. Jaržemskas and others who achieved major developments of practical and academic surgery, scientific works, organization of the Lithuanian Society of Surgeons and establishment of the first Lithuanian periodic medical journal „Medicina“. Some interesting facts about the public and social roles of those prominent personalities and the tragedy many of them lived at this turn of history are also presented.
POLA ASUH ANAK DALAM KELUARGA PEMULUNG
Authors: Ma'rifah Inayati , Nourwahida Cut Dhien , Adriansyah Andri Noor
Published: January 2019

Abstract.The study of child care in the family scavenger in Jurang Mangu Timur South Tangerang. The goal is to find out how this kind of upbringing applied parents who work as scavengers. The research method using qualitative methods, and the type of research is descriptive. Researchers took the data by interviewing, observation and documentation. Informant was a parent who worked as scavengers and their children aged 4-12 years old. The results showed that parenting in a family scavenger leads to authoritarian upbringing. That pattern of care that have rigid rules in raising children. Each violation subject to physical punishment, verbal and psychological. Rarely praise or reward obtained when children obey this parents. Few or never existed to justify the praise children's behaviour.  when they implement the rules. Low income levels have influenced parenting applied to the scavenger families. Income earned by only family scavenger Rp.400.000.00 until Rp600.000.00 every month, because of a low income, they even employ their children find stuff. They do not spoil the child, and also rare to spoil a child. Abstrak. Studi pengasuhan anak di pemulung keluarga di Jurang Mangu Timur Tangerang Selatan. Tujuannya adalah untuk mengetahui bagaimana cara pengasuhan yang diterapkan orangtua yang bekerja sebagai pemulung. Metode penelitian menggunakan metode kualitatif, dan jenis penelitiannya adalah deskriptif. Peneliti mengambil data dengan wawancara, observasi dan dokumentasi. Informan adalah orang tua yang bekerja sebagai pemulung dan anak-anak mereka yang berusia 4-12 tahun. Hasil penelitian menunjukkan bahwa mengasuh anak dalam pemulung keluarga mengarah pada pengasuhan yang otoriter. Itulah pola asuh yang memiliki aturan kaku dalam membesarkan anak. Setiap pelanggaran dikenakan hukuman fisik, verbal dan psikologis. Jarang pujian atau hadiah didapat saat anak-anak mematuhi orang tua ini. Beberapa atau tidak pernah ada untuk membenarkan perilaku pujian anak-anak. Ketika mereka menerapkan aturan. Tingkat pendapatan yang rendah telah memengaruhi pola asuh yang diterapkan pada keluarga pemulung. Penghasilan yang diperoleh hanya oleh pemulung keluarga Rp.400.000,00 hingga Rp600.000,00 setiap bulan, karena penghasilan yang rendah, mereka bahkan mempekerjakan anak-anak mereka mencari barang. Mereka tidak memanjakan anak, dan juga jarang memanjakan anak.
Program Sekolah dalam Upaya Pencegahan NAPZA
Authors: Fidrayani Fidrayani , Utami Desiana
Published: January 2019

Abstract. Narcotics in unattended use can have a negative impact. The impact is potential to destroy the youth generation. It is necessary to hold the drug prevention programs, especially in schools as one of ways to prevent the widespread negative impact. The prevention can be through the role of parents, teachers and stakeholders as part of the community. The teachers’ role is divided into four sub-role, namely as educators, as advisors, as mentors, and particularly as problem solvers. The role of teachers certainly will not have power if it is not supported by the role of schools. Effots that are conducted by school stakeholder are as follow: a) compose the school rules; b) conduct inspections intensively and spontaneously; c) cooperate with related institutions for counseling and socialization; d) utilize extracurricular activities as means of prevention; e) integrate drug awareness into learning curriculum; (f) maximize the tasks as the effort of drug abuse prevention. Expectedly, these drug prevention efforts can be implemented by all schools as one of ways to prevent the destruction of the nation through NAPZA. Abstrak. Narkoba dalam penggunaan yang tidak diawasi dapat menimbulkan dampak negatif. Dampak tersebut tidak menutup kemungkinan dapat merusak generasi muda. Salah satu cara agar bahaya tersebut tidak meluas dampaknya, maka perlu program pencegahan terutama di sekolah. Pencegahan tersebut dapat melalui peran orang tua, guru dan pemangku kebijakan sebagai bagian dari masyarakat. Peran guru dibagi dalam empat yakni sebagai pendidik, penasehati, pembimbing dan lebih utama lagi sebagai problem solver. Peran guru ini tentu tidak akan memiliki kekuatan jika tidak didukung oleh peran sekolah. Upaya yang dilakukan oleh pihak sekolah adalah a) membuat tata tertib sekolah, b) melakukan razia intensif dan spontan, c) bekerjasama dengan instansi terkait guna penyuluhan dan sosialisasi, d) menggunakan ekstrakurikuler sebagai sarana pencegahan, e) memasukkan materi tentang narkotika ke dalam kurikulum pengajaran, f) memaksimalkan tugas dalam upaya pencegahan penyalahgunaan NAPZA. Diharapkan agar upaya pencegahan tersebut dapat diikuti oleh semua sekolah sebagai salah satu usaha untuk mencegah rusaknya anak bangsa melalui NAPZA.
Effects of porcine granulocyte-macrophage-colony-stimulating factor on porcine cumulus-oocyte complex maturation in vitro
Authors: Dang Long Thanh , Le Thu-Thao Thi , Huynh Cam Manh , Hoang Son Nghia
Published: January 2019

Introduction: The in vitro maturation (IVM) of oocytes is an essential step in the in vitro embryo production. This study aimed to improve the efficacy of IVM by supplementing with granulocytemacrophage colony stimulating factor (GM-CSF) in the culture medium.

Methods: The combinatorial effects of GM-CSF and porcine follicular fluid (pFF) were also investigated in porcine cumulusoocyte complex (COC) models. COCs were obtained by aspirating follicles with an 18 G needles. Small and medium follicles were used in this study. COCs from two kinds of follicles were divided into 13 groups to investigate the maturation of COCs at different concentrations of pFF (0, 10%) and GM-CSF (0, 2, 10, 20 ng/mL). The maturation of COCs was evaluated by different variables including cumulus expansion, the appearance of the first polar body, lipid droplet localization, mitochondrial distribution, and cortical granule distribution.

Results: The results showed that GM-CSF improves the cumulus expansion, and stimulate the mitochondrial and cortical granule movement. However, GM-CSF did not increase nuclear maturation or lipid droplet localization rates in the porcine oocyte.

Conclusion: In conclusion, porcine GM-CSF had some positive effects on the porcine oocyte maturation.
Método de combinación de pronósticos usando modelos Bayesianos y una metaheurística, caso de estudio
Authors: Higuita-Alzate David , Valencia-Cárdenas Marisol , Correa-Morales Juan Carlos
Published: January 2019

La planeación de pronósticos de demanda de productos perecederos es importante para todo tipo de industria que los manufacture o distribuya, en especial, cuando ésta tiene un comportamiento estacional y variabilidad difícil de predecir. En este trabajo se propone una metaheurística basada en Optimización por Colonia de Hormigas (ACO) para la combinación de pronósticos de múltiples productos, basada en tres modelos: Modelo Lineal Mixto (MLM), Modelo de Regresión Bayesiana (BRM) y Modelo Bayesiano Lineal Dinámico (BDLM), los cuales hacen parte de la combinación propuesta cuyo proceso se basa en la minimización del indicador de Media de Error Absoluto Porcentual Simétrico (SMAPE). Se encuentra que las metodologías de BDLM y de BRM obtienen buenos resultados de forma individual siendo mejor esta última, no obstante, el algoritmo ACO diseñado arroja un mejor resultado, facilitando una adecuada predicción de demanda de varios productos de una empresa del sector de cárnicos.
Decreasing the load on the lower back with an ergonomic cutting tool for harvesting oil palm bunches
Authors: Parra Lina , Quintero Leonardo , Maradei Garcia María Fernanda
Published: January 2019

Harvesting palm oil crops involve different risk factors that decrease workers’ safety and physical integrity. This paper compares stress on the lower back when using the conventional tool and a new cutting system for cutting bunches. The biomechanical analysis is carried out using simulations generated by JACK Siemens software. The study found a 95.97% reduction of intradiscal compression (L4/L5) when cutting bunches and showed that pressure on all joints was reduced, in certain cases, such as the back and elbow, by up to 100%. That is, when cutting using the new system, the worker maintains a neutral posture of the spine. The new design comprises a point of support on which the tool rests and means that workers only have to direct the blade. Future studies should compare both tools in terms of productivity while preserving the ergonomic characteristics.
Methodology for the design of automotive HUD graphical interfaces
Authors: Villa-Espinal Jesús , Osorio-Gómez Gilberto
Published: January 2019

The use of mobile devices inside vehicles while driving is generating an imminent safety risk. Due to this situation, one of the most relevant solutions is the Head-Up Display (HUD) system, which displays information about the system, status, and aids of the vehicle and a little data of the in-vehicle entertainment in the driver’s field of view during the driving activity. In fact, it is important to establish some guidelines or parameters to design HUD interfaces, since the automakers do not disclose their guidelines for the overall design of these interfaces. The main approach of this article is to propose a methodology for the design of automotive HUD interfaces, considering information from the ADAS, IVIS and external devices that meet the current needs of drivers. A validation of the methodology was made with product design engineers and computer engineers. It was concluded that predominantly in the designs the classification of information depending on the level of importance is clear. In this case, driving information is the most relevant in the whole design. An important aspect concerning the implementation of HUD devices is that this technology has more relevance in automotive interior design looking for a perfect balance between the primary task, driving, and the secondary task, multitasking activities on nomadic devices.
Reduction of heavy oil viscosity through ultrasound cavitation assisted by NiO nanocrystals-functionalized SiO2 nanoparticles
Authors: Montes Daniel , Cortés Farid B. , Franco Camilo A
Published: January 2019

The objective of this study is to reduce heavy oil viscosity through the catalytic decomposition of heavy fractions by ultrasound cavitation using metal oxide nanoparticles and water as a hydrogen donor, leading to the reduction of asphaltene content through its conversion into lighter components. NiO nanoparticles were synthesized over a 7 nm silica support using the incipient wetness technique. Emulsified heavy oil (HO) with 40%v/v of water and 13°API was used to evaluate the ultrasound cavitation process over different exposure times and nanoparticle dosages. The viscosity of the emulsified HO before and after ultrasound cavitation was measured with and without nanoparticles. Significant viscosity reduction was obtained, showing best results at 90 minutes of ultrasound exposure time with a nanoparticle dosage of 2000 mg/L, leading to a viscosity reduction at 10 s-1 and 25°C, and an asphaltene content reduction of 44 and 16%, respectively.
Physicomechanical behavior of composites of polypropylene, and mineral fillers with different process cycles
Authors: Caicedo Carolina , Vázquez-Arce Aldo Rafael , Ossa Omar Hernán , De La Cruz Hever , Maciel-Cerda Alfredo
Published: January 2019

In this work, a development of composites of polypropylene [PP] with mineral fillers [M] of talc and calcium carbonate [CaCO3] by co-extrusion and injection techniques were carried out. In the preparation of the mixtures, was used the rheometric analysis to define the optimum temperature of the extrusion process, and a weight ratio of 80:20 PP: fillers was maintained, while for the injection molding process six generations of PP and its compounds were obtained to study the rheological, thermal, morphological and mechanical properties of the new series of PPnM composites formed from a recycled matrix and the PPMn series reprocessed compounds for up to six cycles. The results allowed correlating the changes due to the thermal history and the influence of adding the mineral fillers. The mechanical characterization in the reprocessed matrix indicated a 6.0% decrease in tensile strength and an increase in flexural strength of 9.9%. Likewise, the compounds showed an increase in tensile strength of 11.7%, while flexural strength reached 35.8%. From the thermogravimetric analysis, the degradation temperature in the matrix gradually decreased from 406.5 °C to 364.3 °C, for the sixth generation with respect to the virgin material by the injection process; meanwhile, for the compounds was maintained around 410 °C indicating an optimal interaction, these results could be contrasted with the colorimetric analysis. Finally, re-injection led to a significant decrease in the size of the talc and CaCO3 particles; the sizes were estimated from microstructural analysis from Scanning Electron Microscope.
Técnicas para determinar toxicidad en aguas residuales industriales contaminadas con colorantes y pigmentos
Authors: Cardona-Gallo Santiago Alonso , Agudelo Edison Alexander , Gaviria-Restrepo Luisa Fernanda , Barrios-Ziolo Leonardo Fabio
Published: January 2019

El deterioro del medio ambiente es un tema de gran preocupación actual y que ha impulsado el desarrollo de investigaciones para remediar los efectos nocivos causados por el hombre con su actividad industrial desbordada. Los cuerpos de agua se están viendo afectados por los vertimientos contaminantes de las industrias que utilizan colorantes y pigmentos, ya que es una alteración a los sistemas acuáticos por la interferencia del paso de la luz y las partículas potencialmente tóxicas que tienen efectos no solo sobre los organismos acuáticos sino también posibles efectos indirectos en la salud humana. Esta investigación se desarrolla para conocer y elegir las técnicas de medición de toxicidad más adecuadas para evaluar el impacto de estos efluentes cargados de colorantes y pigmentos para su posterior aplicación a muestras de agua residual reales. Se realiza una revisión bibliográfica de diferentes técnicas para la evaluación de toxicidad acuática y se resalta la importancia de la aplicación de dichas pruebas en la evaluación de las ventajas y la eficiencia de los tratamientos de remoción de color.
Modelo de dirección estratégica con enfoque de arquitectura empresarial para la integración del sistema de dirección de la empresa (deae-isde)
Authors: Malleuve-Martínez Annette , Alfonso-Robaina Daniel , Lavandero-García José , Ramos-Díaz Vivian Cristina
Published: January 2019

Actualmente muchas empresas tienen la necesidad de adoptar modelos y herramientas flexibles que permitan fortalecer la integración corporativa a partir de la gestión de variables de Tecnologías de la Información (TI). El desempeño de las variables asociadas a la gestión de TI incide directamente en el rendimiento de la empresa y en sus relaciones internas y externas. En esta investigación se propone un modelo de dirección estratégica que permite evaluar variables de Arquitectura Empresarial como enfoque de gestión de las TI para contribuir a la Integración del Sistema de Dirección de la Empresa. Su carácter adaptable permitió valorar y comparar resultados de desempeño de los sistemas de dirección en empresas cubanas de diferentes sectores.
Obtaining minimally processed strawberry (Fragaria x ananassa) products and their physicochemical, microbiological, and sensory characterization by using edible coatings
Authors: Gil-Giraldo Erica Yulieth , Duque-Cifuentes Alba Lucia , Quintero-Castaño Victor Dumar
Published: January 2019

The use of edible coatings (EC) has played an important role in the food industry, proving effective in fruit conservation. The objective of this work was to evaluate the effect of edible coatings on a set of physicochemical, microbiological, and sensory parameters of strawberry by using chitosan, calcium caseinate, and low-methoxyl pectin combined with glycerol as plasticizer, ascorbic acid and citric acid as antioxidant agents; uncoated strawberries were used as control. Analyses were performed on days 0, 3, 6, 9, and 12 of storage at 5 °C, evaluating physicochemical and microbiological properties. It was found that the samples with edible coatings differed significantly from the control in the parameters evaluated. The EC1 (chitosan) was effective in prolonging the shelf life of strawberry for up to 14 days, conserving physical and chemical attributes during storage.
Working memory and intelligence quotient: Which best predicts on school achievement?
Authors: Siquara Gustavo Marcelino , Lima Cassio dos Santos , Abreu Neander
Published: January 2019

Working memory (WM) refers to ability to store and manipulate information for a period of time. There is evidence of a close relationship between WM and learning. The aim of this study was to investigate the relationship between WM, intelligence quotient (IQ) on school achievement. Individual neuropsychological assessment was conducted following a specific protocol. The study included 227 children aged 7 to 12 years (M = 9.87, SD = 1.34), of which 119 were females, from private and public schools in Salvador, Bahia, Brazil. The data analysis included descriptive and inferential statistics. Academic achievement was evaluated using the Academic Performance Test (TDE), with subtests in reading, writing and arithmetic. The components of WM were evaluated using backward and forward Digit Span tasks and the backward and forward Corsi block-tapping task. The estimated IQ was assessed using tasks vocabularies and block design of the Wechsler scales. The statistical tests used were linear regression analysis (stepwise) and Pearson correlation. The results showed that the best predictors of academic performance were Digit Span Forward, Corsi Block Backward and Digit Span Backward task. The hypothesis of non-multicollinearity was tested, and it was verified that the constructs were independent (VIF < 10 and Tolerance > 0.20). The variance in academic performance explained by WM model was ΔR = 0.36. High and significant correlations were observed between the components of WM and the TDE. The WM score (Digit Span Backward + Corsi Block Backward) and the TDE were correlated (r = 0.54**). These results indicate that WM is a good predictor of academic achievement than IQ and are consistent with other findings showing WM as a predictor of learning or the potential for learning. This result has important implications for education, particularly with respect to intervention.
Perspectivas e desafios da coordenação de um programa de educação conjugal
Authors: Neumann Angélica Paula , Wagner Adriana
Published: January 2019

O estudo investiga as perspectivas e os desafios em coordenar o programa psicoeducativo para casais “Viver a dois: Compartilhando este desafio”. Foram realizadas entrevistas em grupo com dez equipes de profissionais que conduziram o programa, totalizando 28 participantes. Os dados foram analisados por meio de Análise Temática no software NVivo. Os temas identificados denotam desafios na condução deste programa, como o estabelecimento da aliança terapêutica e o manejo de ocorrências imprevistas durante as oficinas, bem como a manutenção do equilíbrio entre o cumprimento das atividades conforme previsto pelo manual do programa e o estilo próprio de cada moderador. O programa também foi avaliado como um espaço que favorece novas possibilidades de trabalho com casais, em um formato lúdico e participativo. A possibilidade de oferecer uma intervenção de prevenção e promoção da saúde conjugal amplia o repertório de ações disponíveis aos profissionais que atuam nos mais diferentes contextos de trabalho.
Apresentação - Dossiê: Partidos e representação política
Authors: Madeira Rafael Machado , Quadros Marcos Paulo Dos Reis , Roeder Karolina Mattos , Zuccolotto Vinícius Rodrigues
Published: January 2019

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The Effect of Organizational Compensation and Commitment to Organizational Citizenship Behavior in the Cooperative and Small, Middle Enterprises Department of Bali Province
Authors: Suryani Ni Nyoman , Gama I Gede , Parwita Gde Bayu Surya
Published: January 2019

Organizational Citizenship Behavior (OCB) is a behavior which is the initiative of individuals, who are no relation to the formal reward system but was able to increase the effectiveness of the organization. This research was conducted at the Department of cooperatives and SMEs of the province of Bali using 89 employees as research respondents. The technique of determination of the sample used in this study is a research technique in the census. Data was collection by interviews and questionnaires. Data analysis techniques used in this research is the analysis of multiple linear regression that is processed using SPSS software. Based on the results of research that the compensation effect positively and significantly to organizational citizenship behavior. These results have meaning if the compensation received by the employees is increasing then the organizational citizenship behavior will experience an increase. The commitment of the Organization in a positive and significant effect against the organizational citizenship behavior. These results have meaning if the commitment of the Organization owned by employees increased then the organizational citizenship behavior will experience an increase. Compensation and organizational commitment effect simultaneously against the organizational citizenship behavior. These results have meaning if the employee received compensation increases and employee-owned organization commitment was increasing then the organizational citizenship behavior owned employees will experience increased.
Geschnitzt, gestickt, gemalt wirkstrategien spätmittelalterlicher andachtsbilder
Authors: Strohmaier Patricia
Published: January 2019

Carved, embroidered, painted. Effectual strategies of late medieval devotional images. Based on the assumption that the late medieval tension between the desire for God’s grace (Heilssehnsucht) and the uncertainty of it (Heilsunsicherheit) influenced devotional art, the article focuses on three late medieval images designed for private devotional use. A prayer nut carved from boxwood, a triptych embroidered with silk, gold and silver, and the Leipzig Man of Sorrows by the so-called Master Francke all show a popular image of the late Middle Ages, the Man of Sorrows surrounded by the instruments of the Passion. They all make use of techniques to create both closeness and distance to what they represent. The images pull the beholder close through a multitude of details, a realistic style and the illusion of the Man of Sorrows being palpably present, to the point of triggering an impulse to touch him. At the same time, they fail to provide the closeness they suggest. The border between the beholder’s sphere and that of the Christ represented, marked by the necessity to open the objects first, cannot be crossed. Blurred details, overlappings and the impossibility to touch the fine artworks, lest they be impaired, push the beholder away, leaving the contemplation of the presented image to his or her inner eye instead. This fine balance designed to induce devotion gets lost in the modern era when the images’ skilfulness becomes the main motive for admiration.
Cover_Belakang
Authors: Subandriyo, ST Joko
Published: January 2019

Cover_Belakang
Microbial Production of 2,3- Butanediol From Waste Glycerol
Authors: Yankova S. , Begova P. , Beschkov V.
Published: January 2019

An inevitable waste from biodiesel production is glycerol, contaminated by alkaline catalyst, water and methanol and released in quite big amounts, exceeding its market demand. One way of its utilization is production of different bulk and fine chemicals, for example 2,3- butanediol. The latter is used as a precursor for manufacturing of plastics, synthetic rubber, some pharmaceuticals, etc. In the present paper the ability of the strain Klebsiella oxytoca VA 8391 to produce this compound under batch and fed-batch conditions was studied at initial glycerol concentrations between 10 and 30 g dm-3. Experiments have been carried out in shaking flasks. The bacteria cultivation was at 37oC and stirred conditions at 200 rpm, whereas the product formation was studied both under stirred and still conditions. It was found out that the used strain is capable to produce selectively 2,3- butanediol with very high yields of practical importance (up to 92 %) and no contamination by other products. The optimum glycerol concentration was 20 g dm-3. At higher initialconcentrations substrate inhibition started to occur. It was established that the studied fermentation process required aerobic bacteria cultivation followed by anoxic period of product formation by the developed culture. Only in this case high yields were attained.
New Heterostructures on the Basis of Cd1-xMnxTe Solid Solutions: Photoelectric Properties
Authors: Rud Vasiliy , Rud Yury , Il’chuk Grygory , Kusnezh Volodymyr , Petrus’ Roman
Published: January 2019

The new technology of energy barrier fabrication by thermal treatment of Cd1-xMnxTe solid solution crystal wafers was proposed. By the first time the Ox/Cd1-хMnхTe (х=0.00–0.70) heterostructures with rectifying and photosensitive properties was fabricated. The relative quantum efficiency of photoconversion of fabricated by the firs time heterostructures was investigated. The nature of the interband optical transitions and values of the band gap in Cd1-xMnxTe was determined.
Diagnosis and Treatment Monitoring of a Patient with Gastrointestinal Stromal Tumor by Next-Generation Sequencing and Droplet Digital Polymerase Chain Reaction Assay of a PDGFRA Mutation in Plasma-Derived Cell-Free Tumor DNA
Authors: Boonstra Pieter A. , ter Elst Arja , Tibbesma Marco , Gietema Jourik A. , Schuuring Ed , Reyners Anna K.L.
Published: January 2019

In patients with a suspected malignancy, standard-of care management currently includes histopathologic examination and analysis of tumor-specific molecular abnormalities. Herein, we present a 77-year-old patient with an abdominal mass suspected to be a gastrointestinal stromal tumor (GIST) but without the possibility to collect a tumor biopsy. Cell-free DNA extracted from a blood sample was analyzed for the presence of mutations in GIST-specific genes using next generation sequencing. Furthermore, liquid biopsies were used to monitor the levels of mutant DNA copies during treatment with a tumor-specific mutation droplet digital PCR assay that correlated with the clinical and radiological response. Blood-based testing is a good alternative for biopsy-based testing. However, it should only be applied when biopsies are not available or possible to obtain because overall, in only 50%–85% of the cell-free plasma samples is the known tumor mutation detected.
Supplemental materials
Authors: Warren David E , Duff Mellissa C , Tranel Daniel , Cohen Neal J
Published: January 2019

This item supplements the article "Observing Degradation of Visual Representations over Short Intervals When Medial-temporal Lobe Is Damaged" Journal of Cognitive Neuroscience , Volume 23, Issue 12, December 2011 p.3862-3873. PMID: 21736458. https://doi.org/10.1162/jocn_a_00089