bluuebunny/crossref_metadata_2025 huggingface dataset:
Can Organic Matter from Waste-Derived Amendments Limit Phosphorus Losses from Soil to the Aquatic Environment?
Authors: Szara Ewa , Kwiatkowska-Malina Jolanta , Malina Grzegorz
Published: February 2023

When introducing innovative waste-derived amendments, their potential impact on soil retention capacity towards phosphorus (P) should be accounted for to limit its losses and thus water eutrophication. The study was aimed at evaluating P sorption properties of organic waste-derived amendments conifer woodchip biochar (BIO) and brown coal waste (BCW), and assessing their impact on P sorption properties in soil five years after their application in terms of limiting P losses from soils to the water sources. In batch tests samples of amendments and sandy acid soil (Haplic Luvisol) were exposed for 24 h to balancing solutions with a different P content, respectively 0–80 mg P L−1 for amendments and 0–20 mg P L−1 for soil. Three treatments were studied: (1) conifer woodchip biochar (BIO, (2) brown coal waste (BCW) and (0) soil without amendment (control). Organic amendments were applied to soil at a rate equivalent of 10.2 t C ha−1 once at the start of the trial and they amounted to 24.2 t ha−1 for BCW and 12.8 t ha−1 for BIO. Based on the results of the bath tests and the Langmuir and Freundlich equations, sorption parameters of P were determined for BIO, BCW and the soil after their application. The maximum sorption capacities (Smax) of BIO and BCW were 832.3 and 250.4 mg kg−1, respectively, and were controlled mainly by the organic surfaces of their structures. Within the range of concentrations used in the balancing solutions (5–20 mg P L−1), sorption of P was generally higher in unfertilized soil compared to soil amended with BCW and BIO. The application of BIO significantly (p < 0.05) decreased the Smax (17%), while BCW decreased the bonding energy of P (k) by 15% compared to the untreated soil. The content of dissolved P increased significantly in the case of both BCW and BIO applications, which may indicate an increase in soil susceptibility to P losses. However, the effectiveness of these amendments in limiting P losses with the use of other doses and in different soil and climatic conditions cannot be excluded. Further research is recommended to better understand the effect of biochar feedstock and temperature of its production on P retention/losses in various types of soils.
Effect of Irrigation, Nitrogen Fertilization and Amino Acid Biostimulant on Proximate Composition and Energy Value of Pisum sativum L. Seeds
Authors: Biel Wioletta , Podsiadło Cezary , Witkowicz Robert , Kępińska-Pacelik Jagoda , Stankowski Sławomir
Published: February 2023

The study investigated the impact of biological and agricultural conditions on the chemical composition and energy value of pea seeds for poultry feed. In the experiment, the species assessed was pea (Pisum sativum L.), a determinate form, cultivar (cv.) Cysterski. During the field experiment, the response of peas to the following experimental factors was assessed: first factor—irrigation (yes, no), second factor—nitrogen fertilization (0, 20 and 40 kg N·ha−1), third factor—amino acid biostimulant (yes, no). In both years of experiments, representative seed samples were collected, in which the chemical composition was assessed for the content of dry matter, crude protein, crude fat, crude fiber, and nitrogen free extract. Additionally, in order to assess the suitability of the evaluated pea seeds as poultry feed, the energy value expressed in the form of apparent metabolizable energy (AMEn) was estimated. The protein content in pea seeds increased after the application of the biostimulant, both with and without irrigation. Statistically significant differences in the crude fiber content in pea seeds were found under the influence of the interaction of irrigation and nitrogen fertilization. The use of the biostimulant significantly increased the energy value of pea seeds.
Design and Test of Self-Leveling System for Cleaning Screen of Grain Combine Harvester
Authors: Wu Jun , Tang Qing , Mu Senlin , Yang Xiaoxuan , Jiang Lan , Hu Zhichao
Published: February 2023

As one of the core working parts of a combine harvester, the cleaning device directly affects the operation performance of the whole machine. This is especially the case on hilly and gently sloping terrain, which, due to the uneven ground, causes the combine harvester body to incline and material to accumulates on one side, resulting in a high cleaning loss rate. To solve this problem, a self-leveling cleaning screen device and a control system based on a fuzzy PID control algorithm are developed for a caterpillar harvester, enabling it to operate on gentle slopes of 10°. To verify the performance of the fuzzy PID algorithm applied to this system, simulation tests, response tests, comparison tests, and field tests were carried out. The indoor test results show that the system has a good tracking effect when the inclination amplitude does not exceed 10°. The maximum leveling error is −0.62°, the maximum leveling time is 1.85 s, and the maximum overshoot is 1.5°. The field test results show that when the tilt angle of the harvester body is within 10°, the system can stabilize the real-time leveling of the cleaning screen. Even with an increase in the tilt angle of the harvester, the cleaning loss of the harvester installed with the automatic leveling system can still be maintained at a low level. The cleaning loss rate of the harvester is 1.2% higher after leveling than during flat operation, which meets the accuracy requirements of the system design. Therefore, this system can be applied to grain combine harvesters and effectively reduce the cleaning loss caused by their operation.
Geographical Indication, Agricultural Products Export and Urban–Rural Income Gap
Authors: Zhang Sihui , Sun Yong , Yu Xuzhou , Zhang Yafeng
Published: February 2023

The Nineteenth National Congress of the Communist Party of China put forward the implementation of a rural revitalization strategy, which is an important way to achieve common prosperity for all the people, as promoting farmers’ income increase and narrowing the urban–rural income gap are key to promoting rural revitalization and common prosperity. So, under the background of vigorously promoting the rural revitalization strategy in China, it is very important to explore the effect and realization mechanism of geographical indication (GI) on reducing the urban–rural income gap. Based on the statistical data of 31 provinces in China from 2008 to 2019, this empirical study uses the spatial Durbin model (SDM) to analyze the relationship between GI and urban–rural income gap, and the stepwise regression method is used to explore the mediating effect of agricultural product exports on it. The results show that: (1) The potential economic value of GI branding can reduce the urban–rural income gap, and each additional unit of GI in this region will reduce the urban–rural income difference of this region by 0.160 units, and the urban–rural income difference of neighboring regions by 0.133 units. The result is still consistent after changing the proxy variable of urban–rural income gap for robustness test; (2) The brand effect of GI can form a stronger competitive advantage in foreign trade and promote the export level of agricultural products; (3) GI can narrow the urban–rural income gap through the export of agricultural products, and agricultural product export plays an important mediating effect. In the future, the government should not only strengthen the management and protection of GI but also actively market GI products. Promoting the international mutual recognition and mutual protection of GI can ensure the agricultural product export of GI and improve the foreign trade level of GI.
Feeding Mixed Silages of Winter Cereals and Italian Ryegrass Can Modify the Fatty Acid and Odor Profile of Bovine Milk
Authors: Yakubu Haruna Gado , Ali Omeralfaroug , Szabó András , Tóth Tamás , Bazar George
Published: February 2023

The utilization of corn silage in animal diets is becoming a challenge, due to the crop’s reduced yield as a result of climate change. Alternative silage types, such as mixtures of Italian ryegrass and winter cereals, may be a good complement to corn silage in diet formulation. Therefore, it is important to investigate how these alternative sources influence milk fatty acid and odor profile, as well as how these quality parameters could be efficiently evaluated. In this study, a corn silage-based control (CTR) and four experimental (EXP) diets—which contained winter cereals (WC), as well as WC with Italian ryegrass (IRG) silages in different proportions—were fed to Holstein-Friesian cows (n = 32) in a single-blinded efficacy study during a series of 4-week periods, with 2 weeks of adaption to each feed before the main trial. Milk from each trial was subjected to fatty acid (FA) analysis and odor profiling through the utilization of gas chromatography and an electronic nose, respectively. The results show that milk FAs in the EXP-3 and EXP-4 groups (which contained mixed silages using WC) changed the most when compared with other groups. Moreover, with a 7 kg/day inclusion rate of WC + IRG and of the WC silages in the diets of the EXP-2 and EXP-3 groups, respectively, the milk from these groups had their n6:n3 ratio reduced, thus indicating possible health benefits to consumers. The odor variation between the milk of the WC + IRG and WC groups was greater than the variation between the milk of the CTR and EXP groups. The main volatile compound responsible for the odor of the CTR milk was ethyl-butyrate, whereas 2-propanol and butan-2-one dominated the WC milk; the milk samples of the WC + IRG groups were influenced largely by ethanol. The study proved that with a 7 kg/day inclusion of mixed silages including winter cereals plus Italian ryegrass, the FA and odor profile of bovine milk could be modified.
Exploring the Potential of White-Rot Fungi Exudates on the Amelioration of Salinized Soils
Authors: Borges João , Cardoso Paulo , Lopes Isabel , Figueira Etelvina , Venâncio Cátia
Published: February 2023

Soil salinization has a major impact on crop revenue, which may be counteracted by harnessing the microbiota that the soil itself harbors. This study aimed to explore the application of fungi exudates (Trametes versicolor and Pleurotus sajor caju) on the potential relief of salt stress in the performance of Lens culinaris in two different soils (a silvopastoral agroforestry soil and LUFA 2.2). Three salinity levels (8, 16, and 26 mS·cm−1) and three levels of each fungal exudate (1%, 6%, and 12%) were evaluated in a 96-h cross-design experiment. Seed germination was checked daily, and after 96-h, seedling radicle and hypocotyl were measured, along with fresh and dry weights and soil phosphatase activity. The sole application of exudates from neither fungus affected the germination and seedling development of L. culinaris. Salinity alone induced a dose-effect response in all L. culinaris tested endpoints, as expected because conductivities above 8 mS·cm−1 are known to be detrimental for many crop species. Application of exudates to salinized soils improved germination rates at 16 and 26 mS·cm−1 compared to the single respective salinity treatment alone, but mainly in assays carried out in natural soil. In addition, under the same conditions (26 mS·cm−1 and all fungi %), there was an improvement in natural soil acid phosphatase activity. The application of rot fungi exudates demonstrates potential in what might prove to be a sustainable measure to reclaim salinized soils and improve crop productivity, which is consistent with current soil protection policies.
Non-Thermal Plasma-Activated Water: A Cytogenotoxic Potential on Triticum aestivum
Authors: Padureanu Silvica , Burlica Radu , Stoleru Vasile , Beniuga Oana , Dirlau Delicia , Cretu Daniel Eusebiu , Astanei Dragos , Patras Antoanela
Published: February 2023

Non-thermal plasma-activated water (PAW) is used in agriculture to decontaminate the seed/grains surface, with possible positive effects on physiological processes. In the present study, PAW was generated in ambient air at atmospheric pressure in eight variants with pH and different doses of reactive species (H2O2, NO3−). We explored the indirect effect of PAW on wheat grains while focusing on genetic material by cytogenetic monitoring. All PAW variants caused clastogenic and aneugenic events of the genetic material, with different intensities, in a dose-dependent manner of reactive species in plasma composition. PAW with the highest doses of H2O2 (13–22 mg/L) and NO3− (49–68 mg/L) at pH 3.8–4.1 decreased the mitotic index the most and induced the most frequent genetic abnormalities, out of which chromosomal bridges and micronuclei were dominant. In correlation with this damage at the nucleus level, the germination rate and root and shoot length of wheat sprouts decreased significantly only in the variants with the highest doses of reactive species. PAW with the lowest doses of H2O2 (1–5 mg/L) and NO3− (8–15 mg/L) at pH 5.5–5.1 induced a much lower cytogenotoxic potential, maintained a germination rate comparable to the control and even significantly stimulated root and shoot length growth. Thus, the effects of PAW depend highly on the dose of reactive species and on pH.
Bioactive Compounds Assessment in Six Moroccan Rapeseed (Brassica napus L.) Varieties Grown in Two Contrasting Environments
Authors: Guirrou Ibtissame , El Harrak Abdelhay , El Antari Abderraouf , Hssaini Lahcen , Hanine Hafida , El Fechtali Mohamed , Nabloussi Abdelghani
Published: February 2023

Rapeseed (Brassica napus L.) is the world’s second-largest oilseed crop after soybean. It contains functional compounds, including fatty acids and phenols, which are known for their health and nutritional benefits. In Morocco, rapeseed was introduced as a promising oilseed crop that has shown good adaptation and great potential. Six genotypes were registered and released as the most high-performance Moroccan varieties in terms of both seed yield and oil content. Apart from their ‘00′ quality, i.e., oil without erucic acid and meal with very low glucosinolate content, there is no information on other important quality traits. Therefore, this research aimed to characterize the lipochemical and phenolic attributes of those varieties, namely Baraka, Narjisse, Moufida, Lila, Alia, and Adila, grown at two contrasting sites (Allal Tazi and Douyet) so as to assess the environmental impact on oil quality. A set of 14 parameters were analyzed, comprising seed yield, oil content (Oil C), protein content (PC), acidity index (IA), peroxide index (IP), refractive index (IR), iodine value (IO), total phenolic content (TPC), total flavonoids, total carotenoid contents, free-radical scavenging activity (FRSA), half-maximal inhibitory concentration, fatty acid composition, and omega 6/omega 3 ratio. The results displayed significant differences (p < 0.001) between the two sites for all the abovementioned traits, except for IA, with an outperformance of Allal Tazi over Douyet. Additionally, variety and variety by site interaction had a significant effect on all the studied parameters, except for IA, IP, IR, and TPC. Over both environments, the varietal effect generated variations in yield of 10.9 to 17.1 q/ha, Oil C of 36.7 to 39.9%, PC of 21.3 to 25.9%, IO of 94.1 to 100 g I2/100 g, FRSA of 22.7 to 42.9%, and an omega6/omega3 ratio of 1.8 to 2.3%. It is noteworthy that the Moufida and Alia varieties displayed a low-magnitude effect of the environment, as they maintained similar high performance over both sites. They, thus, represent Moroccan genetic material of interest from an agronomic and nutritional perspective. Therefore, they should be promoted and encouraged for cultivation in Morocco, mainly in the Allal Tazi area and similar regions.
Optimizing Row Spacing Increases Stalk Lodging Resistance by Improving Light Distribution in Dense Maize Populations
Authors: Jin Rong , Li Zhong , Wang Xinglong , Liu Fan , Kong Fanlei , Liu Qinlin , Lan Tianqiong , Feng Dongju , Yuan Jichao
Published: February 2023

Dense planting effectively increases maize yield while increasing stalk lodging risk. Appropriate row spacing can improve the maize population structure and stalk lodging resistance, but its physiological ecological mechanisms and interaction with planting density are unclear. Here, a two-year field experiment to determine the joint effects of row spacing and planting density on maize stem characteristics and the quantitative relationship of the light condition within a maize population with stalk lodging resistance indicated that the stalk mechanical strength showed a quadratic function relationship with photosynthetically active radiation (PAR), whereas the lodging rate showed an exponential function relationship with basal light transmittance (LT). Further, the basal LT was significantly positively correlated with basal internode thickness, dry weight per unit stem length (DWUL), mechanical and cortical tissue thickness, and lignin and cellulose contents. Increasing the planting density decreased the basal LT and PAR; correspondingly decreased the basal internode thickness, DWUL, mechanical and cortical tissue thickness, lignin and cellulose contents, and stalk mechanical strength; and increased the lodging rate, while increasing row spacing did the opposite. Thus, optimizing the row spacing enhanced the lodging resistance through LT and PAR improvement of the lower part of the population and further increased the grain yield by optimizing the yield components. The appropriate row spacing varied with the planting density. The proper strategy for high stalk lodging resistance and grain yielding under this experimental condition was 67,500 plants ha−1 density with 60 + 60 cm equal row spacing.
Assessing Grapevine Water Status by Integrating Vine Transpiration, Leaf Gas Exchanges, Chlorophyll Fluorescence and Sap Flow Measurements
Authors: Benyahia Fadwa , Bastos Campos Flávio , Ben Abdelkader Ahmed , Basile Boris , Tagliavini Massimo , Andreotti Carlo , Zanotelli Damiano
Published: February 2023

A precise knowledge of the grapevine responses to increasing level of water stress is of the utmost relevance for an effective application of deficit irrigation strategies in viticulture. Against this background, a study was conducted on potted grapevines subjected to two ten-day drought cycles to assess their water status by integrating the information derived from different physiological indexes including whole-plant transpiration (measured gravimetrically and with sap flow sensors), leaf gas exchanges and chlorophyll fluorescence. When soil water availability was not limited, vine transpiration rate was determined mainly by environmental factors and ranged between 0.5 and 2 L day−1 m−2 of leaf surface. Transpiration was affected by the soil water availability only when water stress became evident (midday stem water potential < −1 MPa), with vines showing a strong limitation of the stomata functioning (stomatal conductance < 0.05 mol m−2 s−1) and, consequently, low transpiration rates (<0.5 L m−2 d−1). Transpiration rates measured with the sap flow sensors were correlated with those measured gravimetrically, showing daily patterns that were highly affected by the intensity of the water stress. Nevertheless, these sensors highly underestimated actual transpiration rates, limiting their reliability for vineyard irrigation management. At the end of the second drought cycle, vines showed very limited responses to daily changes in environmental conditions (same photosynthetic rate and stomatal conductance at morning, midday and afternoon), likely reflecting a carryover stress effect from the first drought cycle and an incomplete physiological recovery before the beginning of the second. Evidence of the severe water stress reached by vines was also given by the high value of the quantum yield of nonregulated energy dissipation (Y(NO) higher than 0.4) found at the end of the first drought cycle. Taken together, the obtained results integrate the current knowledge on water stress development in grapevine, also highlighting the relevance of specific physiological indexes that could be used effectively for the correct management of deficit strategies in viticulture.
Elevated Temperature Affects Avena sterilis ssp. ludoviciana Reproductive Biology
Authors: Ali Mohammad , Williams Alwyn , Widderick Michael , Adkins Steve
Published: February 2023

The weed Avena sterilis ssp. ludoviciana has a high economic impact in the winter cereal crop production systems of Australia’s northern grains region (NGR). In the NGR, the frequency of high-temperature periods at the end of winter is increasing. This shift in climate may modify this weed’s maturity time and reproductive biology, and thereby impact on crop production. This study examined the reproductive biology of four A. ludoviciana biotypes in relation to elevated temperature when applied at different times during their seed development. Plants of all four A. ludoviciana biotypes were grown in an ambient temperature glasshouse (23/14 °C day/night). At panicle initiation, a portion of the plants were transferred to an elevated temperature glasshouse (29/23 °C day/night) and remained there until maturity. This process of plant movement was repeated on three further occasions with separate batches of plants, each 10 days apart. The remaining plants were kept under ambient conditions for their whole lifespan. Plants exposed to elevated temperature from panicle initiation to maturity, matured 18 days earlier than plants kept under ambient conditions, had 30% fewer filled seeds, 37% lower seed mass, and 40% less seed dormancy. Depending on the time and duration of plants exposed to elevated temperature, predicted seed longevity was ranged from 1 to 4 years in the soil seedbank. All reproductive traits were less affected when plants were exposed to elevated temperature at a later stage of development. If the frequency of high-temperature periods continues to increase, then it may lead to the development of less dormant populations of this weed that would be ready to germinate and re-infest the next winter crops under no-tillage conservation agriculture (that does not bury seeds deep in the soil profile). However, the seasonal climatic variability of the NGR in addition to the weed’s natural genetic variability may contribute to a seedbank of both dormant and less dormant seeds—making this species an even more difficult-to-control weed.
Hemp Agronomy: Current Advances, Questions, Challenges, and Opportunities
Authors: Sunoj Valiaparambil Sebastian John , Dong Xuejun , Trostle Calvin , Pham Hanh , Joshi Madhumita V. , Jessup Russell W. , Burow Mark D. , Provin Tony L.
Published: February 2023

Hemp (Cannabis sativa L. ssp. sativa) has a long history of domestication due to its versatile use. Recently, different sectors in the economy are investigating hemp cultivation to increase agronomic production and to limit delta-9-tetrahydrocannabinol (THC). Despite the rapid growth of hemp literature in recent years, it is still uncertain whether the knowledge gained from higher latitude regions is applicable to low latitude and tropical regions where hemp has not been grown traditionally. This review provides a comprehensive and updated survey of hemp agronomy, focusing on environmental and management factors influencing the growth and yield of hemp, methods of cannabinoids detection and quantification, and hemp breeding. This review suggests that some previous claims about hemp as a low input crop may not hold true in low-latitude regions. Additional research strategies, such as the integration of experimentation and modeling efforts, are encouraged to hasten new discoveries. Furthermore, to effectively increase the outputs of value products (cannabinoids, seeds, fiber and biomass, etc.) while limiting the THC level, new collaborations between hemp agronomists and economists may streamline the production process by increasing the efficiency of the total production system of hemp as a multifaceted crop.
An Enveloping, Centering, and Grabbing Mechanism for Harvesting Hydroponic Leafy Vegetables Cultivated in Pipeline
Authors: Liu Yuanjie , Xia Hongmei , Feng Junjie , Jiang Linhuan , Li Liuquan , Dong Zhao , Zhao Kaidong , Zhang Jiamou
Published: February 2023

Manually harvesting hydroponic leafy vegetables from a cultivation pipeline is labor-intensive and expensive. Rapidly grabbing hydroponic leafy vegetables grown in different positions and orientations in the planting hole is the primary issue for efficient mechanical harvesting. Thus, a novel grabbing mechanism with double-pivot rotation cross fingers is proposed. The fingers’ inner surfaces could envelop the grabbable area of the leafy vegetable in the grasping process and position each leafy vegetable stalk to the center of the planting hole before taking it out. A grabbing mechanism for harvesting hydroponic Chinese kale was designed and optimized with less than 1 mm of grasping error, enough enveloping range, and no collision with the extended leaves. Laboratory experiments were conducted to investigate centering and grabbing at different initial positions and inclination angles of the hydroponic Chinese kale and varied finger deflection speeds. It was indicated that for the grasping inclination angle and grabbing success rate, the initial inclination angle was a significant factor, as was the position, whereas the finger deflection speed was insignificant. As the initial inclination angle of matured hydroponic Chinese kale in different initial positions is mostly larger than 60°, the best results were achieved with a finger deflection speed in the range of 40° s−1 to 60° s−1 and grasping inclination angles of 85° to 95°, with a grabbing success rate of more than 95%. This showed the promising applicability of the studied grabbing mechanism for harvesting hydroponic Chinese kale or other varieties of leafy hydroponic vegetables with similar growth characteristics.
Social Isolation Does Not Alter Exploratory Behaviour, Spatial Learning and Memory in Captive Damaraland Mole-Rats (Fukomys damarensis)
Authors: Blecher Arantxa Silvia , Oosthuizen Maria Kathleen
Published: February 2023

Exploratory behaviour, spatial learning and memory affect the survival of animals and appear to be dependent on the specific habitat that a species occupies. Good spatial navigation and memory are particularly important for subterranean animals, as it is energetically expensive to inhabit this niche. Damaraland mole-rats are subterranean mammals that live in colonies with organised social structures. Damaraland mole-rats have been maintained in the laboratory for many years and can be housed in groups or individually. We evaluated the effect of social isolation on the exploratory behaviour and spatial memory of single-housed and colony-housed animals and also considered potential differences in animals with different social statuses. We predicted that solitary housing would increase anxiety-like behaviour and result in higher activity and more errors when solving a maze. Exploration by colony- and single-housed mole-rats was tested in an open-field test, where all individuals explored readily. Single-housed queens and non-breeding females showed increased activity and spent more time in tunnels, which can be explained by increased anxiety. In the Y-maze, improvements in solving the maze were observed in all experimental groups, except in single-housed non-breeding females. In addition, all males showed a decrease in the number of errors in the maze. Spatial learning is thus apparent but could not be conclusively proven. It was possibly underestimated, as magnetic cues that may be used by mole-rats as stimuli for navigation were removed in the experimental setup. Overall, it appears that social isolation has a limited effect on the exploratory behaviour and spatial learning of Damaraland mole-rats.
African Elephant Milk Short Saccharide and Metabolite Composition and Their Changes over Lactation
Authors: Osthoff Gernot , Wiese Irenie , Deacon Francois
Published: February 2023

Elephant milk composition is unique, as are its changes over lactation. Presented here is the milk non-dedicated metabolite composition of three African elephants. Their lactation times are overlapping and span day one to thirty months. Metabolites were identified and quantified by 1H nuclear magnetic resonance spectroscopy. Lactose and short oligosaccharides are a large component of the metabolites, with lacto-N-difucohexaose I as the major oligosaccharide. These were followed by metabolites of lipids, amino acids, and the citric acid cycle. The content of lactose, lacto-N-difucohexaose I, 2′-fucosyllactose, and some unidentified oligosaccharides decrease over lactation, while that of difucosyllactose and other unidentified ones increase. The high content of glutamate, as a glucogenic amino acid, supported the uprated synthesis of saccharides by the milk gland cells. The content of succinate and choline increase over lactation, indicating higher energy expenditure and phospholipid synthesis during later lactation.
The Effect of Combining Millet and Corn Straw as Source Forage for Beef Cattle Diets on Ruminal Degradability and Fungal Community
Authors: Tong Yaoyi , Wu Jincai , Guo Wenwei , Yang Zhimin , Wang Haocheng , Liu Hongkai , Gao Yong , Sun Maohong , Yue Chunwang
Published: February 2023

Three ruminal cannulated Simmental crossbreed bulls (approximately 3 years of age and with 380 ± 20 kg live weight at initiation of the experiment) were used in a 3 × 3 Latin square experiment in order to determine the effects of the treatments on ruminal pH and degradability of nutrients, as well as the rumen fungal community. The experimental periods were 21 d, with 18 d of adjustment to the respective dietary treatments and 3 d of sample collection. Treatments consisted of a basal diet containing a 47.11% composition of two sources of forage as follows: (1) 100% millet straw (MILLSTR), (2) 50:50 millet straw and corn straw (COMB), and (3) 100% corn straw (CORNSTR). Dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), and acid detergent fiber (ADF) were tested for ruminal degradability using the nylon bag method, which was incubated for 6, 12, 24, 36, 48, and 72 h, and rumen fungal community in rumen fluid was determined by high-throughput gene sequencing technology. Ruminal pH was not affected by treatments. At 72 h, compared to MILLSTR, DM degradability of CORNSTR was 4.8% greater (p < 0.05), but when corn was combined with millet straw, the difference in DM degradability was 9.4%. During the first 24 h, degradability of CP was lower for CORNSTR, intermediate for MILLSTR, and higher for COMB. However, at 72 h, MILLSTR and COMB had a similar CP degradability value, staying greater than the CP degradability value of the CORNSTR treatment. Compared to MILLSTR, the rumen degradability of NDF was greater for CORNSTR and intermediate for the COMB. There was a greater degradability for ADF in CORNSTR, intermediate for COMB, and lower for MILLSTR. In all treatments, Ascomycota and Basidiomycota were dominant flora. Abundance of Basidiomycota in the group COMB was higher (p < 0.05) than that in the group CORNSTR at 12 h. Relative to the fungal genus level, the Thelebolus, Cladosporium, and Meyerozyma were the dominant fungus, and the abundance of Meyerozyma in COMB and CORNSTR were greater (p < 0.05) than MILLSTR at 12, 24, and 36 h of incubation. In conclusion, it is suggested to feed beef cattle with different proportions of millet straw and corn straw combinations.
Oral Sampling of Little Brown Bat (Myotis lucifugus) Maternity Colonies for SARS-CoV-2 in the Northeast and Mid-Atlantic, USA
Authors: Moran Megan L. , Boyd William , De La Cruz Jesse L. , Bertke Andrea S. , Ford W. Mark
Published: February 2023

The potential introduction of SARS-CoV-2, the virus responsible for the COVID-19 pandemic, into North American bat populations is of interest to wildlife managers due to recent disease-mediated declines of several species. Populations of little brown bats (Myotis lucifugus) have collapsed due to white-nose syndrome (WNS), a disease caused by the introduction and spread of the fungal pathogen Pseudogymnoascus destructans (Pd). Throughout much of the United States and southern Canada, large colonies of the species routinely established diurnal roosts in anthropogenic structures, thereby creating the potential for direct human contact and cross-species disease transmission. Given recent declines and the potential for further disease impacts, we collected oral swabs from eight little brown bat maternity colonies to assess the presence and prevalence of SARS-CoV-2 by RT-qPCR analysis. Little brown bat colonies in Maryland (n = 1), New Hampshire (n = 1), New Jersey (n = 2), New York (n = 1), Rhode Island (n = 2), and Virginia (n = 1) were taken during May-August, 2022. From 235 assayed individuals, no bat tested positive for SARS-CoV-2. Our results indicate that little brown bats may not contract SARS-CoV-2 or that the virus persists at undetectable levels in populations of the Mid-Atlantic and Northeast during summer months. Nonetheless, continued monitoring and future work addressing other seasons may still be warranted to conclusively determine infection status.
Growth Stages and Inter-Species Gut Microbiota Composition and Function in Captive Red Deer (Cervus elaphus alxaicus) and Blue Sheep (Pseudois nayaur)
Authors: Zhao Yao , Sun Jia , Ding Mengqi , Hayat Khattak Romaan , Teng Liwei , Liu Zhensheng
Published: February 2023

Blue sheep and red deer, second-class key protected animals in China, are sympatric species with a high degree of overlap of food resources in the Helan Mountains, China. Previous studies with blue sheep and red deer in nature have shown that their physiology is closely related to their gut microbiota. However, growth stages and changes occurring in these species in captivity are still unknown. Thus, 16S rRNA gene sequencing was used to explore diversity, composition and function of the gut microbiota in these two animal species. The diversity and structure of the gut microbiota in captive blue sheep and red deer changed at different growth stages, but the dominant microbiota phyla in the gut microbiota remained stable, which was composed of the phyla Firmicutes, Bacteroidetes and Verrucomicrobia. Moreover, gut microbiota diversity in juvenile blue sheep and red deer was low, with the potential for further colonization. Functional predictions showed differences such as red deer transcription being enriched in adults, and blue sheep adults having a higher cell wall/membrane/envelope biogenesis than juveniles. Microbial changes between blue sheep and red deer at different growth stages and between species mainly depend on the abundance of the microbiota, rather than the increase and absence of the bacterial taxa.
Distinguishing the Effects of Water Volumes versus Stocking Densities on the Skeletal Quality during the Pre-Ongrowing Phase of Gilthead Seabream (Sparus aurata)
Authors: Dellacqua Zachary , Di Biagio Claudia , Costa Corrado , Pousão-Ferreira Pedro , Ribeiro Laura , Barata Marisa , Gavaia Paulo J. , Mattei Francesco , Fabris Andrea , Izquierdo Marisol , Boglione Clara
Published: February 2023

Gilthead seabream (Sparus aurata) production is a highly valued aquaculture industry in Europe. The presence of skeletal deformities in farmed gilthead seabream represents a major bottleneck for the industry leading to economic losses, negative impacts on the consumers’ perception of aquaculture, and animal welfare issues for the fish. Although past work has primarily focused on the hatchery phase to reduce the incidence of skeletal anomalies, this work targets the successive pre-ongrowing phase in which more severe anomalies affecting the external shape often arise. This work aimed to test the effects of: (i) larger and smaller tank volumes, stocked at the same density; and (ii) higher and lower stocking densities maintained in the same water volume, on the skeleton of gilthead seabream fingerlings reared for ~63 days at a pilot scale. Experimental rearing was conducted with gilthead seabream juveniles (~6.7 ± 2.5 g), which were selected as ‘non-deformed’ based on external inspection, stocked at three different densities (Low Density (LD): 5 kg/m3; Medium Density (MD): 10 kg/m3; High Density (HD): 20 kg/m3) in both 500 L and 1000 L tanks. Gilthead seabream were sampled for growth performance and radiographed to assess the skeletal elements at the beginning and end of the experimental trial. Results revealed that (i) LD fish were significantly longer than HD fish, although there were no differences in final weights, regardless of the water volume; (ii) an increase in the prevalence of seabream exhibiting cranial and vertebral axis anomalies was found to be associated with increased density. These results suggest that farmers can significantly reduce the presence of some cranial and axis anomalies affecting pre-ongrown gilthead seabream by reducing the stocking density.
Evaluation of Muscle Proteins for Estimating the Post-Mortem Interval in Veterinary Forensic Pathology
Authors: Piegari Giuseppe , De Pasquale Valeria , d’Aquino Ilaria , De Biase Davide , Caccia Giulia , Campobasso Carlo Pietro , Tafuri Simona , Russo Valeria , Paciello Orlando
Published: February 2023

Postmortem cadaveric changes are commonly used to estimate the postmortem interval (PMI) in humans and animals. However, these modifications have been poorly investigated in animals of interest to veterinary forensic pathology. The aim of this study was to investigate the potential use of muscle proteins (desmin and dystrophin) as biomarkers for estimating the PMI in dogs. For this study, 10 dead adult dogs were evaluated for 4 days in a temperature-controlled room at 19 ± 1 °C. For each animal, at 3, 24, 48, 72, and 96 h after death, a 1 × 1 × 1 cm cube of muscle tissue was removed from the vastus lateralis and triceps brachii. Protein expression levels were analyzed by immunohistochemical examination and immunoblot analysis. The obtained results showed rapid dystrophin degradation, with complete disappearance at 72 h after death. In contrast, desmin-positive fibers and desmin protein bands detected by immunoblot were observed on all 4 days of observation. Our findings suggest the potential use of muscle proteins as biomarkers for estimating the PMI in dogs.
Characterization of an IDH1 R132H Rabbit Monoclonal Antibody, MRQ-67, and Its Applications in the Identification of Diffuse Gliomas
Authors: Copaciu Raul , Rashidian Juliet , Lloyd Joshua , Yahyabeik Aril , McClure Jennifer , Cummings Kelsea , Su Qin
Published: February 2023

The current diagnosis of diffuse glioma involves isocitrate dehydrogenase (IDH) mutation testing. Most IDH mutant gliomas carry a G-to-A mutation at IDH1 position 395, resulting in the R132H mutant. R132H immunohistochemistry (IHC), therefore, is used to screen for the IDH1 mutation. In this study, the performance of MRQ-67, a recently generated IDH1 R132H antibody, was characterized in comparison with H09, a frequently used clone. Selective binding was demonstrated by an enzyme-linked immunosorbent assay for MRQ-67 to the R132H mutant, with an affinity higher than that for H09. By Western and dot immunoassays, MRQ-67 was found to bind specifically to the IDH1 R1322H, with a higher capacity than H09. IHC testing with MRQ-67 demonstrated a positive signal in most diffuse astrocytomas (16/22), oligodendrogliomas (9/15), and secondary glioblastomas tested (3/3), but not in primary glioblastomas (0/24). While both clones demonstrated a positive signal with similar patterns and equivalent intensities, H09 exhibited a background stain more frequently. DNA sequencing on 18 samples showed the R132H mutation in all IHC positive cases (5/5), but not in negative cases (0/13). These results demonstrate that MRQ-67 is a high-affinity antibody suitable for specific detection of the IDH1 R132H mutant by IHC and with less background as compared with H09.
The Clash of the Titans: COVID-19, Carbapenem-Resistant Enterobacterales, and First mcr-1-Mediated Colistin Resistance in Humans in Romania
Authors: Miftode Ionela-Larisa , Leca Daniela , Miftode Radu-Stefan , Roşu Florin , Plesca Claudia , Loghin Isabela , Timpau Amalia Stefana , Mitu Ivona , Mititiuc Irina , Dorneanu Olivia , Miftode Egidia
Published: February 2023

(1) Background: Antibiotic resistance and coronavirus disease-19 (COVID-19) represent a dual challenge in daily clinical practice, inducing a high burden on public health systems. Hence, we aimed to dynamically evaluate the impact of COVID-19 on patients with carbapenem-resistant Enterobacterales (CRE) urinary tract infections (UTIs), as well as the antibiotic resistance trends after the onset of the pandemic. (2) Methods: We conducted a prospective study including patients with CRE UTIs who were enrolled both pre- and during the pandemic from 2019 to 2022. We further performed a standardized and comparative clinical, paraclinical, and microbiological assessment between patients with and without COVID-19. (3) Results: A total of 87 patients with CRE UTIs were included in this study (46 pre-pandemic and 41 during the pandemic, of which 21 had associated Severe Acute Respiratory Syndrome Coronavirus-2 infection). Klebsiella pneumoniae was the main etiological agent of the UTIs, with the majority of strains (82.7%) being carbapenemase producers (mainly OXA-48 producers), while five of the 34 colistin-resistant isolates were harboring the mobile colistin resistance-1 (mcr-1) gene. COVID-19 patients presented a significantly worse outcome with higher rates of intensive care unit (ICU) admissions (66.7% for COVID patients vs. 18.2% for non-COVID patients, p < 0.001), while the fatality rates were also considerably higher among patients with concomitant viral infection (33.3% vs. 12.1%, p < 0.001). Besides COVID-19, additional risk factors associated with increased mortality were urinary catheterization, sepsis with K. pneumoniae, impaired liver and kidney function, and an inappropriate initial empiric antibiotic therapy. (4) Conclusions: COVID-19 showed a pronounced negative impact on patients with CRE UTIs, with significantly longer hospitalizations and higher ICU admissions and mortality rates.
Antimicrobial Activity of Spices Popularly Used in Mexico against Urinary Tract Infections
Authors: Ortega-Lozano Ariadna Jazmín , Hernández-Cruz Estefani Yaquelin , Gómez-Sierra Tania , Pedraza-Chaverri José
Published: February 2023

Urinary tract infections (UTIs) are the most common infectious diseases worldwide. These infections are common in all people; however, they are more prevalent in women than in men. The main microorganism that causes 80–90% of UTIs is Escherichia coli. However, other bacteria such as Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, Proteus mirabilis, and Klebsiella pneumoniae cause UTIs, and antibiotics are required to treat them. However, UTI treatment can be complicated by antibiotic resistance and biofilm formation. Therefore, medicinal plants, such as spices generally added to foods, can be a therapeutic alternative due to the variety of phytochemicals such as polyphenols, saponins, alkaloids, and terpenes present in their extracts that exert antimicrobial activity. Essential oils extracted from spices have been used to demonstrate their antimicrobial efficacy against strains of pathogens isolated from UTI patients and their synergistic effect with antibiotics. This article summarizes relevant findings on the antimicrobial activity of cinnamon, clove, cumin, oregano, pepper, and rosemary, spices popularly used in Mexico against the uropathogens responsible for UTIs.
Effect of Extracts, Fractions, and Isolated Molecules of Casearia sylvestris to Control Streptococcus mutans Cariogenic Biofilm
Authors: Ribeiro Sabrina M. , Bueno Paula C. P. , Cavalheiro Alberto José , Klein Marlise I.
Published: February 2023

The effects of extracts, fractions, and molecules of Casearia sylvestris to control the cariogenic biofilm of Streptococcus mutans were evaluated. First, the antimicrobial and antibiofilm (initial and pre-formed biofilms) in prolonged exposure (24 h) models were investigated. Second, formulations (with and without fluoride) were assessed for topical effects (brief exposure) on biofilms. Third, selected treatments were evaluated via bacterium growth inhibition curves associated with gene expression and scanning electron microscopy. In initial biofilms, the ethyl acetate (AcOEt) and ethanolic (EtOH) fractions from Brasília (BRA/DF; 250 µg/mL) and Presidente Venceslau/SP (Water/EtOH 60:40 and Water/EtOH 40:60; 500 µg/mL) reduced ≥6-logs vs. vehicle. Only the molecule Caseargrewiin F (CsF; 125 µg/mL) reduced the viable cell count of pre-formed biofilms (5 logs vs. vehicle). For topical effects, no formulation affected biofilm components. For the growth inhibition assay, CsF yielded a constant recovery of surviving cells (≅3.5 logs) until 24 h (i.e., bacteriostatic), and AcOEt_BRA/DF caused progressive cell death, without cells at 24 h (i.e., bactericidal). CsF and AcOEt_BRA/DF damaged S. mutans cells and influenced the expression of virulence genes. Thus, an effect against biofilms occurred after prolonged exposure due to the bacteriostatic and/or bactericidal capacity of a fraction and a molecule from C. sylvestris.
Bats Are Carriers of Antimicrobial-Resistant Staphylococcaceae in Their Skin
Authors: Carrillo Gaeta Natália , Cavalcante Brito João Eduardo , Nunes Batista Juliana Maria , Gagete Veríssimo de Mello Beatriz , Dias Ricardo Augusto , Heinemann Marcos B.
Published: February 2023

Bats have emerged as potential carriers of zoonotic viruses and bacteria, including antimicrobial-resistant bacteria. Staphylococcaceae has been isolated from their gut and nasopharynx, but there is little information about Staphylococcaceae on bat skin. Therefore, this study aimed to decipher the Staphylococci species in bat skin and their antimicrobial susceptibility profile. One hundred and forty-seven skin swabs were collected from bats during the spring and summer of 2021 and 2022. Bats were captured in different areas of the Metropolitan Region of São Paulo, Brazil, according to the degree of anthropization: Area 1 (Forested), Area 2 (Rural), Area 3 (Residential-A), Area 4 (Slum-– up to two floors), Area 5 (Residential-B—condo buildings), and Area 6 (Industrial). Swabs were kept in peptone water broth at 37 °C for 12 h when bacterial growth was streaked in Mannitol salt agar and incubated at 37 °C for 24 h. The disc-diffusion test evaluated antimicrobial susceptibility. Staphylococcaceae were isolated from 42.8% of bats, mostly from young, from the rural area, and during summer. M. sciuri was the most frequent species; S. aureus was also isolated. About 95% of isolates were resistant to at least one drug, and most strains were penicillin resistant. Eight isolates were methicillin resistant, and the mecA gene was detected in one isolate (S. haemolyticus). Antimicrobial resistance is a One Health issue that is not evaluated enough in bats. The results indicate that bats are carriers of clinically meaningful S. aureus and antimicrobial-resistant bacteria. Finally, the results suggest that we should intensify action plans to control the spread of resistant bacteria.